doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text
26957414	15	0	theme	oxonium	1885:1891	arg1	ions					1893:1896	oxonium ions	1885:1896	oxonium ions	1885:1896	The formation of Y1 ions and oxonium ions in ISF facilitates glycopeptide sequencing and glycan composition identification.
26957414	7	1	theme	Y1	928:929	arg1	ions					931:934	dominant Y1 ions	919:934	dominant Y1 ions	919:934	To attain dominant Y1 ions, a range of source fragmentation voltages was studied using fetuin.
26957414	8	2	theme	V	1009:1009	arg1	voltage					1015:1021	A 45 V ISF voltage	1004:1021	A 45 V ISF voltage	1004:1021	A 45 V ISF voltage was found to be the most efficient voltage for the analysis of glycoproteins.
26957414	8	2	theme	V	1009:1009	arg1	voltage					1058:1064	the most efficient voltage	1039:1064	the most efficient voltage for the analysis of glycoproteins	1039:1098	A 45 V ISF voltage was found to be the most efficient voltage for the analysis of glycoproteins.
26957414	12	3	theme	acid	1423:1426	arg1	groups					1428:1433	different sialic acid groups	1406:1433	different sialic acid groups	1406:1433	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	15	4	theme	ions	1876:1879	arg1	formation					1860:1868	The formation	1856:1868	The formation of Y1 ions and oxonium ions in ISF	1856:1903	The formation of Y1 ions and oxonium ions in ISF facilitates glycopeptide sequencing and glycan composition identification.
26957414	5	5	theme	composition	727:737	arg1	information					739:749	glycan composition information	720:749	glycan composition information	720:749	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	6	6	theme	peptide	853:859	arg1	backbone					861:868	peptide backbone	853:868	peptide backbone with an N-acetylglucosamine attached	853:905	In ISF method, the identification of glycosylation sites depended on the detection of Y1 ion (ion of peptide backbone with an N-acetylglucosamine attached).
26957414	4	7	theme	glycopeptides	457:469	arg1	analysis					445:452	LC-MS/MS analysis	436:452	LC-MS/MS analysis of glycopeptides	436:469	LC-MS/MS analysis of glycopeptides is faced with the low glycopeptide signal intensities and low peptide sequence identification.
26957414	9	8	theme	porcine	1224:1230	arg1	thyroglobulin					1232:1244	porcine thyroglobulin	1224:1244	porcine thyroglobulin	1224:1244	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	11	9	gly	glycopeptides	1317:1329	arg2	glycopeptides					1317:1329	glycopeptides	1317:1329	glycopeptides	1317:1329	Y1 ions of glycopeptides in tryptic digests of samples were detected.
26957414	1	10	theme	biological	198:207	arg1	processes					209:217	biological processes	198:217	biological processes	198:217	Glycosylation plays important roles in maintaining protein stability and controlling biological processes.
26957414	12	11	theme	different	1451:1459	arg1	times					1471:1475	different retention times	1451:1475	different retention times	1451:1475	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	12	12	theme	glycopeptides	1387:1399	arg1	ions					1379:1382	Y1 ions	1376:1382	Y1 ions of glycopeptides with different sialic acid groups	1376:1433	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	5	13	theme	backbone	698:705	arg1	sequence					707:714	peptide backbone sequence	690:714	peptide backbone sequence	690:714	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	6	14	theme	ion	841:843	arg1	detection					825:833	the detection	821:833	the detection of Y1 ion	821:843	In ISF method, the identification of glycosylation sites depended on the detection of Y1 ion (ion of peptide backbone with an N-acetylglucosamine attached).
26957414	12	15	located	observed	1439:1446	arg2	ions					1379:1382	Y1 ions	1376:1382	Y1 ions of glycopeptides with different sialic acid groups	1376:1433	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	12	15	located	observed	1439:1446	arg1	times					1471:1475	different retention times	1451:1475	different retention times	1451:1475	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	12	16	theme	different	1406:1414	arg1	groups					1428:1433	different sialic acid groups	1406:1433	different sialic acid groups	1406:1433	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	6	17	with	backbone	861:868	arg1	N-acetylglucosamine					878:896	an N-acetylglucosamine	875:896	an N-acetylglucosamine attached	875:905	In ISF method, the identification of glycosylation sites depended on the detection of Y1 ion (ion of peptide backbone with an N-acetylglucosamine attached).
26957414	13	18	theme	backbone	1621:1628	arg1	sequencing					1630:1639	the peptide backbone sequencing	1609:1639	the peptide backbone sequencing of glycopeptides	1609:1656	With ISF facilitating the peptide backbone sequencing of glycopeptides, identified peptide sequence coverage was increased.
26957414	5	19	with	conjunction	622:632	arg1	LC-MS/MS					639:646	LC-MS/MS	639:646	LC-MS/MS	639:646	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	4	20	theme	glycopeptide	493:504	arg1	intensities					513:523	the low glycopeptide signal intensities	485:523	the low glycopeptide signal intensities	485:523	LC-MS/MS analysis of glycopeptides is faced with the low glycopeptide signal intensities and low peptide sequence identification.
26957414	15	21	gly	glycopeptide	1917:1928	arg2	glycopeptide					1917:1928	glycopeptide sequencing	1917:1939	glycopeptide sequencing	1917:1939	The formation of Y1 ions and oxonium ions in ISF facilitates glycopeptide sequencing and glycan composition identification.
26957414	3	22	theme	qualitative	328:338	arg1	analyses					357:364	qualitative and quantitative analyses	328:364	qualitative and quantitative analyses of glycoproteins	328:381	Hence, qualitative and quantitative analyses of glycoproteins are necessary to understand physiological processes.
26957414	14	23	theme	fetuin	1735:1740	arg1	percentage					1751:1760	identified fetuin sequence percentage	1724:1760	identified fetuin sequence percentage	1724:1760	For example, identified fetuin sequence percentage was improved from 39 to 80% in MASCOT database searching compared to conventional CID method.
26957414	1	24	theme	important	133:141	arg1	roles					143:147	important roles	133:147	important roles	133:147	Glycosylation plays important roles in maintaining protein stability and controlling biological processes.
26957414	9	25	theme	α1-acid	1199:1205	arg1	glycoprotein					1207:1218	α1-acid glycoprotein	1199:1218	α1-acid glycoprotein	1199:1218	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	2	26	theme	many	288:291	arg1	diseases					293:300	many diseases	288:300	many diseases	288:300	In recent years, the correlation between aberrant glycoproteins and many diseases has been reported.
26957414	14	27	dep	80	1786:1787	arg1	to					1783:1784	to	1783:1784	to	1783:1784	For example, identified fetuin sequence percentage was improved from 39 to 80% in MASCOT database searching compared to conventional CID method.
26957414	14	28	from	%	1788:1788	arg1	database					1800:1807	MASCOT database	1793:1807	MASCOT database searching compared to conventional CID method	1793:1853	For example, identified fetuin sequence percentage was improved from 39 to 80% in MASCOT database searching compared to conventional CID method.
26957414	1	29	theme	protein	164:170	arg1	stability					172:180	protein stability	164:180	protein stability	164:180	Glycosylation plays important roles in maintaining protein stability and controlling biological processes.
26957414	5	30	used	used	614:617	arg2	ISF					605:607	ISF	605:607	ISF	605:607	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	5	30	used	used	614:617	arg2	fragmentation					590:602	in-source fragmentation	580:602	in-source fragmentation (ISF)	580:608	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	6	31	gly	glycosylation	789:801	arg2	sites					803:807	glycosylation sites	789:807	glycosylation sites	789:807	In ISF method, the identification of glycosylation sites depended on the detection of Y1 ion (ion of peptide backbone with an N-acetylglucosamine attached).
26957414	3	32	theme	quantitative	344:355	arg1	analyses					357:364	qualitative and quantitative analyses	328:364	qualitative and quantitative analyses of glycoproteins	328:381	Hence, qualitative and quantitative analyses of glycoproteins are necessary to understand physiological processes.
26957414	11	33	theme	Y1	1306:1307	arg1	ions					1309:1312	Y1 ions	1306:1312	Y1 ions of glycopeptides in tryptic digests of samples	1306:1359	Y1 ions of glycopeptides in tryptic digests of samples were detected.
26957414	10	34	used	used	1269:1272	arg2	approach					1251:1258	The approach	1247:1258	The approach	1247:1258	The approach was then used to analyze blood serum samples.
26957414	10	35	theme	blood	1285:1289	arg1	samples					1297:1303	blood serum samples	1285:1303	blood serum samples	1285:1303	The approach was then used to analyze blood serum samples.
26957414	0	36	theme	glycosylation	80:92	arg1	proteins					103:110	proteins	103:110	proteins	103:110	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	0	36	theme	glycosylation	80:92	arg1	sites					94:98	the glycosylation sites	76:98	the glycosylation sites of proteins	76:110	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	8	37	gly	glycoproteins	1086:1098	arg1	glycoproteins					1086:1098	glycoproteins	1086:1098	glycoproteins	1086:1098	A 45 V ISF voltage was found to be the most efficient voltage for the analysis of glycoproteins.
26957414	12	38	theme	peptide	1560:1566	arg1	sequence					1577:1584	the same peptide backbone sequence	1551:1584	the same peptide backbone sequence	1551:1584	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	4	39	theme	low	529:531	arg1	identification					550:563	low peptide sequence identification	529:563	low peptide sequence identification	529:563	LC-MS/MS analysis of glycopeptides is faced with the low glycopeptide signal intensities and low peptide sequence identification.
26957414	4	40	theme	sequence	541:548	arg1	identification					550:563	low peptide sequence identification	529:563	low peptide sequence identification	529:563	LC-MS/MS analysis of glycopeptides is faced with the low glycopeptide signal intensities and low peptide sequence identification.
26957414	0	41	theme	Parallel	0:7	arg1	acquisition					14:24	Parallel data acquisition	0:24	Parallel data acquisition of in-source fragmented glycopeptides	0:62	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	7	42	theme	voltages	969:976	arg1	range					939:943	a range	937:943	a range of source fragmentation voltages	937:976	To attain dominant Y1 ions, a range of source fragmentation voltages was studied using fetuin.
26957414	3	43	theme	physiological	411:423	arg1	processes					425:433	physiological processes	411:433	physiological processes	411:433	Hence, qualitative and quantitative analyses of glycoproteins are necessary to understand physiological processes.
26957414	12	44	theme	moieties	1526:1533	arg1	numbers					1503:1509	the various numbers	1491:1509	the various numbers of sialic acid moieties associated with the same peptide backbone sequence	1491:1584	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	9	45	theme	glycosylation	1131:1143	arg1	sites					1145:1149	the glycosylation sites	1127:1149	the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin	1127:1244	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	45	theme	glycosylation	1131:1143	arg1	glycoproteins					1166:1178	three model glycoproteins	1154:1178	three model glycoproteins	1154:1178	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	0	46	theme	in-source	29:37	arg1	glycopeptides					50:62	in-source fragmented glycopeptides	29:62	in-source fragmented glycopeptides	29:62	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	14	47	theme	conventional	1831:1842	arg1	method					1848:1853	conventional CID method	1831:1853	conventional CID method	1831:1853	For example, identified fetuin sequence percentage was improved from 39 to 80% in MASCOT database searching compared to conventional CID method.
26957414	13	48	theme	identified	1659:1668	arg1	coverage					1687:1694	identified peptide sequence coverage	1659:1694	identified peptide sequence coverage	1659:1694	With ISF facilitating the peptide backbone sequencing of glycopeptides, identified peptide sequence coverage was increased.
26957414	0	49	theme	glycopeptides	50:62	arg1	acquisition					14:24	Parallel data acquisition	0:24	Parallel data acquisition of in-source fragmented glycopeptides	0:62	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	13	50	theme	sequence	1678:1685	arg1	coverage					1687:1694	identified peptide sequence coverage	1659:1694	identified peptide sequence coverage	1659:1694	With ISF facilitating the peptide backbone sequencing of glycopeptides, identified peptide sequence coverage was increased.
26957414	9	51	theme	model	1160:1164	arg1	glycoprotein					1207:1218	α1-acid glycoprotein	1199:1218	α1-acid glycoprotein	1199:1218	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	51	theme	model	1160:1164	arg1	thyroglobulin					1232:1244	porcine thyroglobulin	1224:1244	porcine thyroglobulin	1224:1244	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	51	theme	model	1160:1164	arg1	glycoproteins					1166:1178	three model glycoproteins	1154:1178	three model glycoproteins	1154:1178	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	51	theme	model	1160:1164	arg1	fetuin					1191:1196	fetuin	1191:1196	fetuin	1191:1196	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	7	52	theme	fragmentation	955:967	arg1	voltages					969:976	source fragmentation voltages	948:976	source fragmentation voltages	948:976	To attain dominant Y1 ions, a range of source fragmentation voltages was studied using fetuin.
26957414	12	53	theme	sialic	1514:1519	arg1	moieties					1526:1533	sialic acid moieties	1514:1533	sialic acid moieties associated with the same peptide backbone sequence	1514:1584	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	6	54	dep	depended	809:816	arg1	ion					846:848	ion	846:848	ion of peptide backbone with an N-acetylglucosamine attached	846:905	In ISF method, the identification of glycosylation sites depended on the detection of Y1 ion (ion of peptide backbone with an N-acetylglucosamine attached).
26957414	15	55	theme	ions	1893:1896	arg1	formation					1860:1868	The formation	1856:1868	The formation of Y1 ions and oxonium ions in ISF	1856:1903	The formation of Y1 ions and oxonium ions in ISF facilitates glycopeptide sequencing and glycan composition identification.
26957414	6	56	theme	sites	803:807	arg1	identification					771:784	the identification	767:784	the identification of glycosylation sites	767:807	In ISF method, the identification of glycosylation sites depended on the detection of Y1 ion (ion of peptide backbone with an N-acetylglucosamine attached).
26957414	15	57	theme	composition	1952:1962	arg1	identification					1964:1977	glycan composition identification	1945:1977	glycan composition identification	1945:1977	The formation of Y1 ions and oxonium ions in ISF facilitates glycopeptide sequencing and glycan composition identification.
26957414	5	58	theme	sequence	707:714	arg1	acquisition					675:685	the parallel acquisition	662:685	the parallel acquisition of peptide backbone sequence and glycan composition information	662:749	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	8	59	theme	ISF	1011:1013	arg1	voltage					1015:1021	A 45 V ISF voltage	1004:1021	A 45 V ISF voltage	1004:1021	A 45 V ISF voltage was found to be the most efficient voltage for the analysis of glycoproteins.
26957414	8	59	theme	ISF	1011:1013	arg1	voltage					1058:1064	the most efficient voltage	1039:1064	the most efficient voltage for the analysis of glycoproteins	1039:1098	A 45 V ISF voltage was found to be the most efficient voltage for the analysis of glycoproteins.
26957414	12	60	with	glycopeptides	1387:1399	arg1	groups					1428:1433	different sialic acid groups	1406:1433	different sialic acid groups	1406:1433	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	15	61	theme	Y1	1873:1874	arg1	ions					1876:1879	Y1 ions	1873:1879	Y1 ions	1873:1879	The formation of Y1 ions and oxonium ions in ISF facilitates glycopeptide sequencing and glycan composition identification.
26957414	5	62	theme	glycan	720:725	arg1	composition					727:737	glycan composition	720:737	glycan composition information	720:749	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	6	63	theme	backbone	861:868	arg1	ion					846:848	ion	846:848	ion of peptide backbone with an N-acetylglucosamine attached	846:905	In ISF method, the identification of glycosylation sites depended on the detection of Y1 ion (ion of peptide backbone with an N-acetylglucosamine attached).
26957414	9	64	gly	glycoproteins	1166:1178	arg1	glycoprotein					1207:1218	α1-acid glycoprotein	1199:1218	α1-acid glycoprotein	1199:1218	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	64	gly	glycoproteins	1166:1178	arg1	thyroglobulin					1232:1244	porcine thyroglobulin	1224:1244	porcine thyroglobulin	1224:1244	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	64	gly	glycoproteins	1166:1178	arg1	glycoproteins					1166:1178	three model glycoproteins	1154:1178	three model glycoproteins	1154:1178	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	64	gly	glycoproteins	1166:1178	arg1	fetuin					1191:1196	fetuin	1191:1196	fetuin	1191:1196	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	12	65	theme	sialic	1416:1421	arg1	groups					1428:1433	different sialic acid groups	1406:1433	different sialic acid groups	1406:1433	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	13	66	theme	glycopeptides	1644:1656	arg1	sequencing					1630:1639	the peptide backbone sequencing	1609:1639	the peptide backbone sequencing of glycopeptides	1609:1656	With ISF facilitating the peptide backbone sequencing of glycopeptides, identified peptide sequence coverage was increased.
26957414	7	67	theme	dominant	919:926	arg1	ions					931:934	dominant Y1 ions	919:934	dominant Y1 ions	919:934	To attain dominant Y1 ions, a range of source fragmentation voltages was studied using fetuin.
26957414	12	68	theme	retention	1461:1469	arg1	times					1471:1475	different retention times	1451:1475	different retention times	1451:1475	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	11	69	theme	samples	1353:1359	arg1	digests					1342:1348	tryptic digests	1334:1348	tryptic digests of samples	1334:1359	Y1 ions of glycopeptides in tryptic digests of samples were detected.
26957414	5	70	theme	peptide	690:696	arg1	sequence					707:714	peptide backbone sequence	690:714	peptide backbone sequence	690:714	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	4	71	theme	LC-MS/MS	436:443	arg1	analysis					445:452	LC-MS/MS analysis	436:452	LC-MS/MS analysis of glycopeptides	436:469	LC-MS/MS analysis of glycopeptides is faced with the low glycopeptide signal intensities and low peptide sequence identification.
26957414	2	72	gly	glycoproteins	270:282	arg1	glycoproteins					270:282	aberrant glycoproteins	261:282	aberrant glycoproteins	261:282	In recent years, the correlation between aberrant glycoproteins and many diseases has been reported.
26957414	6	73	theme	Y1	838:839	arg1	ion					841:843	Y1 ion	838:843	Y1 ion	838:843	In ISF method, the identification of glycosylation sites depended on the detection of Y1 ion (ion of peptide backbone with an N-acetylglucosamine attached).
26957414	13	74	theme	peptide	1613:1619	arg1	sequencing					1630:1639	the peptide backbone sequencing	1609:1639	the peptide backbone sequencing of glycopeptides	1609:1656	With ISF facilitating the peptide backbone sequencing of glycopeptides, identified peptide sequence coverage was increased.
26957414	9	75	gly	glycoprotein	1207:1218	arg1	glycoprotein					1207:1218	α1-acid glycoprotein	1199:1218	α1-acid glycoprotein	1199:1218	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	4	76	theme	signal	506:511	arg1	intensities					513:523	the low glycopeptide signal intensities	485:523	the low glycopeptide signal intensities	485:523	LC-MS/MS analysis of glycopeptides is faced with the low glycopeptide signal intensities and low peptide sequence identification.
26957414	15	77	theme	glycopeptide	1917:1928	arg1	sequencing					1930:1939	glycopeptide sequencing	1917:1939	glycopeptide sequencing	1917:1939	The formation of Y1 ions and oxonium ions in ISF facilitates glycopeptide sequencing and glycan composition identification.
26957414	2	78	theme	aberrant	261:268	arg1	glycoproteins					270:282	aberrant glycoproteins	261:282	aberrant glycoproteins	261:282	In recent years, the correlation between aberrant glycoproteins and many diseases has been reported.
26957414	4	79	gly	glycopeptide	493:504	arg2	glycopeptide					493:504	the low glycopeptide signal intensities	485:523	the low glycopeptide signal intensities	485:523	LC-MS/MS analysis of glycopeptides is faced with the low glycopeptide signal intensities and low peptide sequence identification.
26957414	12	80	gly	glycopeptides	1387:1399	arg2	glycopeptides					1387:1399	glycopeptides	1387:1399	glycopeptides with different sialic acid groups	1387:1433	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	5	81	theme	information	739:749	arg1	acquisition					675:685	the parallel acquisition	662:685	the parallel acquisition of peptide backbone sequence and glycan composition information	662:749	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	14	82	theme	sequence	1742:1749	arg1	percentage					1751:1760	identified fetuin sequence percentage	1724:1760	identified fetuin sequence percentage	1724:1760	For example, identified fetuin sequence percentage was improved from 39 to 80% in MASCOT database searching compared to conventional CID method.
26957414	12	83	theme	Y1	1376:1377	arg1	ions					1379:1382	Y1 ions	1376:1382	Y1 ions of glycopeptides with different sialic acid groups	1376:1433	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	14	84	theme	identified	1724:1733	arg1	percentage					1751:1760	identified fetuin sequence percentage	1724:1760	identified fetuin sequence percentage	1724:1760	For example, identified fetuin sequence percentage was improved from 39 to 80% in MASCOT database searching compared to conventional CID method.
26957414	4	85	theme	low	489:491	arg1	intensities					513:523	the low glycopeptide signal intensities	485:523	the low glycopeptide signal intensities	485:523	LC-MS/MS analysis of glycopeptides is faced with the low glycopeptide signal intensities and low peptide sequence identification.
26957414	5	86	theme	in-source	580:588	arg1	fragmentation					590:602	in-source fragmentation	580:602	in-source fragmentation (ISF)	580:608	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	5	86	theme	in-source	580:588	arg1	ISF					605:607	ISF	605:607	ISF	605:607	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	4	87	gly	glycopeptides	457:469	arg2	glycopeptides					457:469	glycopeptides	457:469	glycopeptides	457:469	LC-MS/MS analysis of glycopeptides is faced with the low glycopeptide signal intensities and low peptide sequence identification.
26957414	3	88	theme	glycoproteins	369:381	arg1	analyses					357:364	qualitative and quantitative analyses	328:364	qualitative and quantitative analyses of glycoproteins	328:381	Hence, qualitative and quantitative analyses of glycoproteins are necessary to understand physiological processes.
26957414	15	89	theme	glycan	1945:1950	arg1	identification					1964:1977	glycan composition identification	1945:1977	glycan composition identification	1945:1977	The formation of Y1 ions and oxonium ions in ISF facilitates glycopeptide sequencing and glycan composition identification.
26957414	10	90	theme	serum	1291:1295	arg1	samples					1297:1303	blood serum samples	1285:1303	blood serum samples	1285:1303	The approach was then used to analyze blood serum samples.
26957414	8	91	theme	efficient	1048:1056	arg1	voltage					1058:1064	the most efficient voltage	1039:1064	the most efficient voltage for the analysis of glycoproteins	1039:1098	A 45 V ISF voltage was found to be the most efficient voltage for the analysis of glycoproteins.
26957414	8	91	theme	efficient	1048:1056	arg1	voltage					1015:1021	A 45 V ISF voltage	1004:1021	A 45 V ISF voltage	1004:1021	A 45 V ISF voltage was found to be the most efficient voltage for the analysis of glycoproteins.
26957414	12	92	theme	same	1555:1558	arg1	sequence					1577:1584	the same peptide backbone sequence	1551:1584	the same peptide backbone sequence	1551:1584	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	0	93	theme	proteins	103:110	arg1	proteins					103:110	proteins	103:110	proteins	103:110	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	0	93	theme	proteins	103:110	arg1	sites					94:98	the glycosylation sites	76:98	the glycosylation sites of proteins	76:110	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	12	94	theme	backbone	1568:1575	arg1	sequence					1577:1584	the same peptide backbone sequence	1551:1584	the same peptide backbone sequence	1551:1584	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	4	95	theme	peptide	533:539	arg1	identification					550:563	low peptide sequence identification	529:563	low peptide sequence identification	529:563	LC-MS/MS analysis of glycopeptides is faced with the low glycopeptide signal intensities and low peptide sequence identification.
26957414	5	96	theme	parallel	666:673	arg1	acquisition					675:685	the parallel acquisition	662:685	the parallel acquisition of peptide backbone sequence and glycan composition information	662:749	In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information.
26957414	0	97	gly	glycosylation	80:92	arg1	proteins					103:110	proteins	103:110	proteins	103:110	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	0	97	gly	glycosylation	80:92	arg2	proteins					103:110	proteins	103:110	proteins	103:110	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	0	97	gly	glycosylation	80:92	arg2	sites					94:98	the glycosylation sites	76:98	the glycosylation sites of proteins	76:110	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	11	98	theme	tryptic	1334:1340	arg1	digests					1342:1348	tryptic digests	1334:1348	tryptic digests of samples	1334:1359	Y1 ions of glycopeptides in tryptic digests of samples were detected.
26957414	0	99	theme	data	9:12	arg1	acquisition					14:24	Parallel data acquisition	0:24	Parallel data acquisition of in-source fragmented glycopeptides	0:62	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	13	100	gly	glycopeptides	1644:1656	arg2	glycopeptides					1644:1656	glycopeptides	1644:1656	glycopeptides	1644:1656	With ISF facilitating the peptide backbone sequencing of glycopeptides, identified peptide sequence coverage was increased.
26957414	8	101	theme	glycoproteins	1086:1098	arg1	analysis					1074:1081	the analysis	1070:1081	the analysis of glycoproteins	1070:1098	A 45 V ISF voltage was found to be the most efficient voltage for the analysis of glycoproteins.
26957414	11	102	theme	glycopeptides	1317:1329	arg1	ions					1309:1312	Y1 ions	1306:1312	Y1 ions of glycopeptides in tryptic digests of samples	1306:1359	Y1 ions of glycopeptides in tryptic digests of samples were detected.
26957414	6	103	theme	ISF	755:757	arg1	method					759:764	ISF method	755:764	ISF method	755:764	In ISF method, the identification of glycosylation sites depended on the detection of Y1 ion (ion of peptide backbone with an N-acetylglucosamine attached).
26957414	15	104	from	formation	1860:1868	arg1	ISF					1901:1903	ISF	1901:1903	ISF	1901:1903	The formation of Y1 ions and oxonium ions in ISF facilitates glycopeptide sequencing and glycan composition identification.
26957414	0	105	theme	fragmented	39:48	arg1	glycopeptides					50:62	in-source fragmented glycopeptides	29:62	in-source fragmented glycopeptides	29:62	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	14	106	theme	CID	1844:1846	arg1	method					1848:1853	conventional CID method	1831:1853	conventional CID method	1831:1853	For example, identified fetuin sequence percentage was improved from 39 to 80% in MASCOT database searching compared to conventional CID method.
26957414	12	107	theme	acid	1521:1524	arg1	moieties					1526:1533	sialic acid moieties	1514:1533	sialic acid moieties associated with the same peptide backbone sequence	1514:1584	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	3	108	gly	glycoproteins	369:381	arg1	glycoproteins					369:381	glycoproteins	369:381	glycoproteins	369:381	Hence, qualitative and quantitative analyses of glycoproteins are necessary to understand physiological processes.
26957414	13	109	theme	peptide	1670:1676	arg1	coverage					1687:1694	identified peptide sequence coverage	1659:1694	identified peptide sequence coverage	1659:1694	With ISF facilitating the peptide backbone sequencing of glycopeptides, identified peptide sequence coverage was increased.
26957414	7	110	theme	source	948:953	arg1	voltages					969:976	source fragmentation voltages	948:976	source fragmentation voltages	948:976	To attain dominant Y1 ions, a range of source fragmentation voltages was studied using fetuin.
26957414	11	111	from	ions	1309:1312	arg1	digests					1342:1348	tryptic digests	1334:1348	tryptic digests of samples	1334:1359	Y1 ions of glycopeptides in tryptic digests of samples were detected.
26957414	9	112	gly	glycosylation	1131:1143	arg1	glycoprotein					1207:1218	α1-acid glycoprotein	1199:1218	α1-acid glycoprotein	1199:1218	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	112	gly	glycosylation	1131:1143	arg1	glycoproteins					1166:1178	three model glycoproteins	1154:1178	three model glycoproteins	1154:1178	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	112	gly	glycosylation	1131:1143	arg2	sites					1145:1149	the glycosylation sites	1127:1149	the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin	1127:1244	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	112	gly	glycosylation	1131:1143	arg1	fetuin					1191:1196	fetuin	1191:1196	fetuin	1191:1196	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	112	gly	glycosylation	1131:1143	arg1	thyroglobulin					1232:1244	porcine thyroglobulin	1224:1244	porcine thyroglobulin	1224:1244	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	112	gly	glycosylation	1131:1143	arg2	glycoproteins					1166:1178	three model glycoproteins	1154:1178	three model glycoproteins	1154:1178	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	12	113	theme	various	1495:1501	arg1	numbers					1503:1509	the various numbers	1491:1509	the various numbers of sialic acid moieties associated with the same peptide backbone sequence	1491:1584	Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence.
26957414	2	114	theme	recent	223:228	arg1	years					230:234	recent years	223:234	recent years	223:234	In recent years, the correlation between aberrant glycoproteins and many diseases has been reported.
26957414	9	115	theme	glycoproteins	1166:1178	arg1	sites					1145:1149	the glycosylation sites	1127:1149	the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin	1127:1244	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	9	115	theme	glycoproteins	1166:1178	arg1	glycoproteins					1166:1178	three model glycoproteins	1154:1178	three model glycoproteins	1154:1178	ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin.
26957414	14	116	theme	MASCOT	1793:1798	arg1	database					1800:1807	MASCOT database	1793:1807	MASCOT database searching compared to conventional CID method	1793:1853	For example, identified fetuin sequence percentage was improved from 39 to 80% in MASCOT database searching compared to conventional CID method.
26957414	0	117	gly	glycopeptides	50:62	arg2	glycopeptides					50:62	in-source fragmented glycopeptides	29:62	in-source fragmented glycopeptides	29:62	Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.
26957414	6	118	theme	glycosylation	789:801	arg1	sites					803:807	glycosylation sites	789:807	glycosylation sites	789:807	In ISF method, the identification of glycosylation sites depended on the detection of Y1 ion (ion of peptide backbone with an N-acetylglucosamine attached).
28287093	7	0	from	patients	1380:1387	arg1	HDL					1367:1369	HDL	1367:1369	HDL from HD+ patients	1367:1387	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12) showed that HDL from HD+ patients were enriched in SAA but had lower levels of sialylation across glycoproteins.
28287093	1	1	theme	pilot	188:192	arg1	study					194:198	this pilot study	183:198	this pilot study	183:198	The goal of this pilot study was to determine whether HDL glycoprotein composition affects HDL's immunomodulatory function.
28287093	8	2	theme	glycoprotein	1501:1512	arg1	composition					1514:1524	HDL glycoprotein composition	1497:1524	HDL glycoprotein composition	1497:1524	Our results demonstrate that HDL glycoprotein composition, including the site-specific glycosylation, differentiate between clinical groups, correlate with HDL's immunomodulatory capacity, and may be predictive of HDL's ability to protect from infection.
28287093	6	3	gly	desialylated	1126:1137	arg1	A2HSG					1139:1143	desialylated A2HSG	1126:1143	desialylated A2HSG	1126:1143	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	3	4	theme	α-2-HS-glycoprotein	628:646	arg1	A2HSG					649:653	A2HSG	649:653	A2HSG	649:653	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	4	theme	α-2-HS-glycoprotein	628:646	arg1	Concentrations					445:458	Concentrations	445:458	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG)	445:654	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	4	theme	α-2-HS-glycoprotein	628:646	arg1	glycovariations					679:693	the site-specific glycovariations	661:693	the site-specific glycovariations of ApoC-III, A1AT, and A2HSG	661:722	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	6	5	gly	mono-sialylated	1162:1176	arg1	ApoC-III1					1188:1196	ApoC-III1	1188:1196	ApoC-III1	1188:1196	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	6	5	gly	mono-sialylated	1162:1176	arg1	ApoC-III					1178:1185	mono-sialylated ApoC-III	1162:1185	mono-sialylated ApoC-III (ApoC-III1)	1162:1197	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	1	6	theme	study	194:198	arg1	goal					175:178	The goal	171:178	The goal of this pilot study	171:198	The goal of this pilot study was to determine whether HDL glycoprotein composition affects HDL's immunomodulatory function.
28287093	5	7	from	patients	897:904	arg1	HDL					885:887	HDL	885:887	HDL from HD patients	885:904	HDL from HD patients were enriched in SAA, LBP, ApoC-III, di-sialylated ApoC-III (ApoC-III2) and desialylated A2HSG.
28287093	5	8	theme	desialylated	982:993	arg1	A2HSG					995:999	desialylated A2HSG	982:999	desialylated A2HSG	982:999	HDL from HD patients were enriched in SAA, LBP, ApoC-III, di-sialylated ApoC-III (ApoC-III2) and desialylated A2HSG.
28287093	4	9	from	monocytes	806:814	arg1	assay					843:847	a prototypical assay	828:847	a prototypical assay of HDL's immunomodulatory capacity	828:882	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	4	9	from	monocytes	806:814	arg1	Secretion					739:747	Secretion	739:747	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes	739:814	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	8	10	theme	ability	1688:1694	arg1	predictive					1668:1677	predictive	1668:1677	predictive	1668:1677	Our results demonstrate that HDL glycoprotein composition, including the site-specific glycosylation, differentiate between clinical groups, correlate with HDL's immunomodulatory capacity, and may be predictive of HDL's ability to protect from infection.
28287093	8	11	theme	site-specific	1541:1553	arg1	glycosylation					1555:1567	the site-specific glycosylation	1537:1567	the site-specific glycosylation	1537:1567	Our results demonstrate that HDL glycoprotein composition, including the site-specific glycosylation, differentiate between clinical groups, correlate with HDL's immunomodulatory capacity, and may be predictive of HDL's ability to protect from infection.
28287093	6	12	from	A2HSG	1139:1143	arg1	glycans					1077:1083	di-sialylated glycans	1063:1083	di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG	1063:1143	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	6	12	from	A2HSG	1139:1143	arg1	ApoC-III					1053:1060	ApoC-III	1053:1060	ApoC-III	1053:1060	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	2	13	with	subjects	345:352	arg1	syndrome					369:376	metabolic syndrome	359:376	metabolic syndrome (MetS) (n = 13)	359:392	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	13	with	subjects	345:352	arg1	MetS					379:382	MetS	379:382	MetS	379:382	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	13	with	subjects	345:352	arg1	n = 13					386:391	n = 13	386:391	n = 13	386:391	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	0	14	theme	IL-6	112:115	arg1	Secretion					117:125	IL-6 Secretion	112:125	IL-6 Secretion in Lipopolysaccharide-Stimulated Monocytes	112:168	HDL Glycoprotein Composition and Site-Specific Glycosylation Differentiates Between Clinical Groups and Affects IL-6 Secretion in Lipopolysaccharide-Stimulated Monocytes.
28287093	6	15	theme	glycosylation	1102:1114	arg1	sites					1116:1120	multiple A1AT glycosylation sites	1088:1120	multiple A1AT glycosylation sites	1088:1120	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	7	16	theme	Subgroup	1200:1207	arg1	analysis					1209:1216	Subgroup analysis	1200:1216	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12)	1200:1353	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12) showed that HDL from HD+ patients were enriched in SAA but had lower levels of sialylation across glycoproteins.
28287093	0	17	theme	Lipopolysaccharide-Stimulated	130:158	arg1	Monocytes					160:168	Lipopolysaccharide-Stimulated Monocytes	130:168	Lipopolysaccharide-Stimulated Monocytes	130:168	HDL Glycoprotein Composition and Site-Specific Glycosylation Differentiates Between Clinical Groups and Affects IL-6 Secretion in Lipopolysaccharide-Stimulated Monocytes.
28287093	3	18	theme	site-specific	665:677	arg1	glycovariations					679:693	the site-specific glycovariations	661:693	the site-specific glycovariations of ApoC-III, A1AT, and A2HSG	661:722	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	1	19	gly	glycoprotein	229:240	arg1	glycoprotein					229:240	HDL glycoprotein composition	225:252	HDL glycoprotein composition	225:252	The goal of this pilot study was to determine whether HDL glycoprotein composition affects HDL's immunomodulatory function.
28287093	2	20	theme	HD	422:423	arg1	n = 24					436:441	n = 24	436:441	n = 24	436:441	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	20	theme	HD	422:423	arg1	patients					426:433	diabetic hemodialysis (HD) patients	399:433	diabetic hemodialysis (HD) patients (n = 24)	399:442	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	21	theme	healthy	318:324	arg1	controls					326:333	healthy controls	318:333	healthy controls (n = 13)	318:342	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	21	theme	healthy	318:324	arg1	n = 13					336:341	n = 13	336:341	n = 13	336:341	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	22	theme	diabetic	399:406	arg1	n = 24					436:441	n = 24	436:441	n = 24	436:441	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	22	theme	diabetic	399:406	arg1	patients					426:433	diabetic hemodialysis (HD) patients	399:433	diabetic hemodialysis (HD) patients (n = 24)	399:442	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	7	23	theme	lower	1418:1422	arg1	levels					1424:1429	lower levels	1418:1429	lower levels of sialylation	1418:1444	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12) showed that HDL from HD+ patients were enriched in SAA but had lower levels of sialylation across glycoproteins.
28287093	7	24	gly	glycoproteins	1453:1465	arg1	glycoproteins					1453:1465	glycoproteins	1453:1465	glycoproteins	1453:1465	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12) showed that HDL from HD+ patients were enriched in SAA but had lower levels of sialylation across glycoproteins.
28287093	3	25	theme	protein	523:529	arg1	A2HSG					649:653	A2HSG	649:653	A2HSG	649:653	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	25	theme	protein	523:529	arg1	Concentrations					445:458	Concentrations	445:458	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG)	445:654	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	25	theme	protein	523:529	arg1	glycovariations					679:693	the site-specific glycovariations	661:693	the site-specific glycovariations of ApoC-III, A1AT, and A2HSG	661:722	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	6	26	from	sites	1116:1120	arg1	glycans					1077:1083	di-sialylated glycans	1063:1083	di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG	1063:1143	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	6	26	from	sites	1116:1120	arg1	ApoC-III					1053:1060	ApoC-III	1053:1060	ApoC-III	1053:1060	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	0	27	theme	Glycoprotein	4:15	arg1	Composition					17:27	HDL Glycoprotein Composition	0:27	HDL Glycoprotein Composition	0:27	HDL Glycoprotein Composition and Site-Specific Glycosylation Differentiates Between Clinical Groups and Affects IL-6 Secretion in Lipopolysaccharide-Stimulated Monocytes.
28287093	4	28	theme	lipopolysaccharide-stimulated	776:804	arg1	monocytes					806:814	lipopolysaccharide-stimulated monocytes	776:814	lipopolysaccharide-stimulated monocytes	776:814	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	4	29	used	used	820:823	arg2	Secretion					739:747	Secretion	739:747	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes	739:814	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	4	29	used	used	820:823	arg2	assay					843:847	a prototypical assay	828:847	a prototypical assay of HDL's immunomodulatory capacity	828:882	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	3	30	theme	apolipoprotein	538:551	arg1	ApoA-I					558:563	ApoA-I	558:563	ApoA-I	558:563	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	30	theme	apolipoprotein	538:551	arg1	A-I					553:555	apolipoprotein A-I	538:555	apolipoprotein A-I (ApoA-I)	538:564	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	0	31	theme	HDL	0:2	arg1	Composition					17:27	HDL Glycoprotein Composition	0:27	HDL Glycoprotein Composition	0:27	HDL Glycoprotein Composition and Site-Specific Glycosylation Differentiates Between Clinical Groups and Affects IL-6 Secretion in Lipopolysaccharide-Stimulated Monocytes.
28287093	3	32	theme	ApoC-III	698:705	arg1	A2HSG					649:653	A2HSG	649:653	A2HSG	649:653	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	32	theme	ApoC-III	698:705	arg1	Concentrations					445:458	Concentrations	445:458	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG)	445:654	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	32	theme	ApoC-III	698:705	arg1	glycovariations					679:693	the site-specific glycovariations	661:693	the site-specific glycovariations of ApoC-III, A1AT, and A2HSG	661:722	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	7	33	theme	HD+	1376:1378	arg1	patients					1380:1387	HD+ patients	1376:1387	HD+ patients	1376:1387	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12) showed that HDL from HD+ patients were enriched in SAA but had lower levels of sialylation across glycoproteins.
28287093	7	34	from	analysis	1209:1216	arg1	those					1320:1324	those	1320:1324	those	1320:1324	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12) showed that HDL from HD+ patients were enriched in SAA but had lower levels of sialylation across glycoproteins.
28287093	7	34	from	analysis	1209:1216	arg1	patients					1224:1231	HD patients	1221:1231	HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12)	1221:1313	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12) showed that HDL from HD+ patients were enriched in SAA but had lower levels of sialylation across glycoproteins.
28287093	3	35	theme	A-I	553:555	arg1	A2HSG					649:653	A2HSG	649:653	A2HSG	649:653	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	35	theme	A-I	553:555	arg1	Concentrations					445:458	Concentrations	445:458	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG)	445:654	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	35	theme	A-I	553:555	arg1	glycovariations					679:693	the site-specific glycovariations	661:693	the site-specific glycovariations of ApoC-III, A1AT, and A2HSG	661:722	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	7	36	theme	infectious	1252:1261	arg1	event					1279:1283	an infectious hospitalization event	1249:1283	an infectious hospitalization event	1249:1283	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12) showed that HDL from HD+ patients were enriched in SAA but had lower levels of sialylation across glycoproteins.
28287093	3	37	theme	HDL-bound	463:471	arg1	A					487:487	HDL-bound serum amyloid A	463:487	HDL-bound serum amyloid A (SAA)	463:493	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	37	theme	HDL-bound	463:471	arg1	SAA					490:492	SAA	490:492	SAA	490:492	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	6	38	gly	di-sialylated	1063:1075	arg1	glycans					1077:1083	di-sialylated glycans	1063:1083	di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG	1063:1143	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	6	38	gly	di-sialylated	1063:1075	arg1	ApoC-III					1053:1060	ApoC-III	1053:1060	ApoC-III	1053:1060	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	6	39	theme	di-sialylated	1063:1075	arg1	glycans					1077:1083	di-sialylated glycans	1063:1083	di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG	1063:1143	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	6	39	theme	di-sialylated	1063:1075	arg1	ApoC-III					1053:1060	ApoC-III	1053:1060	ApoC-III	1053:1060	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	6	40	theme	mono-sialylated	1162:1176	arg1	ApoC-III1					1188:1196	ApoC-III1	1188:1196	ApoC-III1	1188:1196	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	6	40	theme	mono-sialylated	1162:1176	arg1	ApoC-III					1178:1185	mono-sialylated ApoC-III	1162:1185	mono-sialylated ApoC-III (ApoC-III1)	1162:1197	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	1	41	theme	HDL	225:227	arg1	composition					242:252	HDL glycoprotein composition	225:252	HDL glycoprotein composition	225:252	The goal of this pilot study was to determine whether HDL glycoprotein composition affects HDL's immunomodulatory function.
28287093	5	42	gly	di-sialylated	943:955	arg1	ApoC-III2					967:975	ApoC-III2	967:975	ApoC-III2	967:975	HDL from HD patients were enriched in SAA, LBP, ApoC-III, di-sialylated ApoC-III (ApoC-III2) and desialylated A2HSG.
28287093	5	42	gly	di-sialylated	943:955	arg1	ApoC-III					957:964	di-sialylated ApoC-III	943:964	di-sialylated ApoC-III (ApoC-III2)	943:976	HDL from HD patients were enriched in SAA, LBP, ApoC-III, di-sialylated ApoC-III (ApoC-III2) and desialylated A2HSG.
28287093	3	43	theme	binding	515:521	arg1	LBP					532:534	LBP	532:534	LBP	532:534	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	43	theme	binding	515:521	arg1	protein					523:529	lipopolysaccharide binding protein	496:529	lipopolysaccharide binding protein (LBP)	496:535	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	44	theme	α-1-antitrypsin	600:614	arg1	A2HSG					649:653	A2HSG	649:653	A2HSG	649:653	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	44	theme	α-1-antitrypsin	600:614	arg1	Concentrations					445:458	Concentrations	445:458	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG)	445:654	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	44	theme	α-1-antitrypsin	600:614	arg1	glycovariations					679:693	the site-specific glycovariations	661:693	the site-specific glycovariations of ApoC-III, A1AT, and A2HSG	661:722	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	5	45	theme	HD	894:895	arg1	patients					897:904	HD patients	894:904	HD patients	894:904	HDL from HD patients were enriched in SAA, LBP, ApoC-III, di-sialylated ApoC-III (ApoC-III2) and desialylated A2HSG.
28287093	6	46	theme	desialylated	1126:1137	arg1	A2HSG					1139:1143	desialylated A2HSG	1126:1143	desialylated A2HSG	1126:1143	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	1	47	theme	glycoprotein	229:240	arg1	composition					242:252	HDL glycoprotein composition	225:252	HDL glycoprotein composition	225:252	The goal of this pilot study was to determine whether HDL glycoprotein composition affects HDL's immunomodulatory function.
28287093	6	48	gly	glycosylation	1102:1114	arg2	sites					1116:1120	multiple A1AT glycosylation sites	1088:1120	multiple A1AT glycosylation sites	1088:1120	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	4	49	theme	capacity	875:882	arg1	assay					843:847	a prototypical assay	828:847	a prototypical assay of HDL's immunomodulatory capacity	828:882	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	4	49	theme	capacity	875:882	arg1	Secretion					739:747	Secretion	739:747	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes	739:814	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	8	50	theme	clinical	1592:1599	arg1	groups					1601:1606	clinical groups	1592:1606	clinical groups	1592:1606	Our results demonstrate that HDL glycoprotein composition, including the site-specific glycosylation, differentiate between clinical groups, correlate with HDL's immunomodulatory capacity, and may be predictive of HDL's ability to protect from infection.
28287093	4	51	theme	interleukin	752:762	arg1	assay					843:847	a prototypical assay	828:847	a prototypical assay of HDL's immunomodulatory capacity	828:882	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	4	51	theme	interleukin	752:762	arg1	Secretion					739:747	Secretion	739:747	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes	739:814	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	8	52	theme	immunomodulatory	1630:1645	arg1	capacity					1647:1654	HDL's immunomodulatory capacity	1624:1654	HDL's immunomodulatory capacity	1624:1654	Our results demonstrate that HDL glycoprotein composition, including the site-specific glycosylation, differentiate between clinical groups, correlate with HDL's immunomodulatory capacity, and may be predictive of HDL's ability to protect from infection.
28287093	7	53	theme	HD	1221:1222	arg1	patients					1224:1231	HD patients	1221:1231	HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12)	1221:1313	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12) showed that HDL from HD+ patients were enriched in SAA but had lower levels of sialylation across glycoproteins.
28287093	0	54	theme	Site-Specific	33:45	arg1	Glycosylation					47:59	Site-Specific Glycosylation	33:59	Site-Specific Glycosylation	33:59	HDL Glycoprotein Composition and Site-Specific Glycosylation Differentiates Between Clinical Groups and Affects IL-6 Secretion in Lipopolysaccharide-Stimulated Monocytes.
28287093	4	55	theme	immunomodulatory	858:873	arg1	capacity					875:882	HDL's immunomodulatory capacity	852:882	HDL's immunomodulatory capacity	852:882	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	7	56	theme	hospitalization	1263:1277	arg1	event					1279:1283	an infectious hospitalization event	1249:1283	an infectious hospitalization event	1249:1283	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12) showed that HDL from HD+ patients were enriched in SAA but had lower levels of sialylation across glycoproteins.
28287093	7	57	theme	sialylation	1434:1444	arg1	levels					1424:1429	lower levels	1418:1429	lower levels of sialylation	1418:1444	Subgroup analysis on HD patients who experienced an infectious hospitalization event within 60 days (HD+) (n = 12), vs. those with no event (HD-) (n = 12) showed that HDL from HD+ patients were enriched in SAA but had lower levels of sialylation across glycoproteins.
28287093	2	58	theme	hemodialysis	408:419	arg1	n = 24					436:441	n = 24	436:441	n = 24	436:441	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	58	theme	hemodialysis	408:419	arg1	patients					426:433	diabetic hemodialysis (HD) patients	399:433	diabetic hemodialysis (HD) patients (n = 24)	399:442	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	3	59	theme	A2HSG	718:722	arg1	A2HSG					649:653	A2HSG	649:653	A2HSG	649:653	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	59	theme	A2HSG	718:722	arg1	Concentrations					445:458	Concentrations	445:458	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG)	445:654	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	59	theme	A2HSG	718:722	arg1	glycovariations					679:693	the site-specific glycovariations	661:693	the site-specific glycovariations of ApoC-III, A1AT, and A2HSG	661:722	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	4	60	theme	prototypical	830:841	arg1	assay					843:847	a prototypical assay	828:847	a prototypical assay of HDL's immunomodulatory capacity	828:882	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	4	60	theme	prototypical	830:841	arg1	Secretion					739:747	Secretion	739:747	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes	739:814	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	5	61	theme	di-sialylated	943:955	arg1	ApoC-III2					967:975	ApoC-III2	967:975	ApoC-III2	967:975	HDL from HD patients were enriched in SAA, LBP, ApoC-III, di-sialylated ApoC-III (ApoC-III2) and desialylated A2HSG.
28287093	5	61	theme	di-sialylated	943:955	arg1	ApoC-III					957:964	di-sialylated ApoC-III	943:964	di-sialylated ApoC-III (ApoC-III2)	943:976	HDL from HD patients were enriched in SAA, LBP, ApoC-III, di-sialylated ApoC-III (ApoC-III2) and desialylated A2HSG.
28287093	2	62	with	patients	426:433	arg1	syndrome					369:376	metabolic syndrome	359:376	metabolic syndrome (MetS) (n = 13)	359:392	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	62	with	patients	426:433	arg1	MetS					379:382	MetS	379:382	MetS	379:382	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	62	with	patients	426:433	arg1	n = 13					386:391	n = 13	386:391	n = 13	386:391	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	3	63	theme	A	487:487	arg1	A2HSG					649:653	A2HSG	649:653	A2HSG	649:653	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	63	theme	A	487:487	arg1	Concentrations					445:458	Concentrations	445:458	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG)	445:654	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	63	theme	A	487:487	arg1	glycovariations					679:693	the site-specific glycovariations	661:693	the site-specific glycovariations of ApoC-III, A1AT, and A2HSG	661:722	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	64	theme	A1AT	708:711	arg1	A2HSG					649:653	A2HSG	649:653	A2HSG	649:653	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	64	theme	A1AT	708:711	arg1	Concentrations					445:458	Concentrations	445:458	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG)	445:654	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	64	theme	A1AT	708:711	arg1	glycovariations					679:693	the site-specific glycovariations	661:693	the site-specific glycovariations of ApoC-III, A1AT, and A2HSG	661:722	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	4	65	from	interleukin	752:762	arg1	monocytes					806:814	lipopolysaccharide-stimulated monocytes	776:814	lipopolysaccharide-stimulated monocytes	776:814	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	3	66	gly	α-2-HS-glycoprotein	628:646	arg1	α-2-HS-glycoprotein					628:646	α-2-HS-glycoprotein	628:646	α-2-HS-glycoprotein	628:646	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	8	67	theme	HDL	1497:1499	arg1	composition					1514:1524	HDL glycoprotein composition	1497:1524	HDL glycoprotein composition	1497:1524	Our results demonstrate that HDL glycoprotein composition, including the site-specific glycosylation, differentiate between clinical groups, correlate with HDL's immunomodulatory capacity, and may be predictive of HDL's ability to protect from infection.
28287093	2	68	theme	metabolic	359:367	arg1	syndrome					369:376	metabolic syndrome	359:376	metabolic syndrome (MetS) (n = 13)	359:392	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	68	theme	metabolic	359:367	arg1	MetS					379:382	MetS	379:382	MetS	379:382	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	68	theme	metabolic	359:367	arg1	n = 13					386:391	n = 13	386:391	n = 13	386:391	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	5	69	gly	desialylated	982:993	arg1	A2HSG					995:999	desialylated A2HSG	982:999	desialylated A2HSG	982:999	HDL from HD patients were enriched in SAA, LBP, ApoC-III, di-sialylated ApoC-III (ApoC-III2) and desialylated A2HSG.
28287093	6	70	theme	multiple	1088:1095	arg1	sites					1116:1120	multiple A1AT glycosylation sites	1088:1120	multiple A1AT glycosylation sites	1088:1120	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	0	71	from	Secretion	117:125	arg1	Monocytes					160:168	Lipopolysaccharide-Stimulated Monocytes	130:168	Lipopolysaccharide-Stimulated Monocytes	130:168	HDL Glycoprotein Composition and Site-Specific Glycosylation Differentiates Between Clinical Groups and Affects IL-6 Secretion in Lipopolysaccharide-Stimulated Monocytes.
28287093	8	72	gly	glycoprotein	1501:1512	arg1	glycoprotein					1501:1512	HDL glycoprotein composition	1497:1524	HDL glycoprotein composition	1497:1524	Our results demonstrate that HDL glycoprotein composition, including the site-specific glycosylation, differentiate between clinical groups, correlate with HDL's immunomodulatory capacity, and may be predictive of HDL's ability to protect from infection.
28287093	4	73	from	Secretion	739:747	arg1	monocytes					806:814	lipopolysaccharide-stimulated monocytes	776:814	lipopolysaccharide-stimulated monocytes	776:814	Secretion of interleukin 6 (IL-6) in lipopolysaccharide-stimulated monocytes was used as a prototypical assay of HDL's immunomodulatory capacity.
28287093	3	74	theme	amyloid	479:485	arg1	A					487:487	HDL-bound serum amyloid A	463:487	HDL-bound serum amyloid A (SAA)	463:493	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	74	theme	amyloid	479:485	arg1	SAA					490:492	SAA	490:492	SAA	490:492	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	75	theme	serum	473:477	arg1	A					487:487	HDL-bound serum amyloid A	463:487	HDL-bound serum amyloid A (SAA)	463:493	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	75	theme	serum	473:477	arg1	SAA					490:492	SAA	490:492	SAA	490:492	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	6	76	theme	IL-6	1021:1024	arg1	secretion					1026:1034	IL-6 secretion	1021:1034	IL-6 secretion	1021:1034	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	3	77	theme	apolipoprotein	567:580	arg1	C-III					582:586	apolipoprotein C-III	567:586	apolipoprotein C-III (ApoC-III)	567:597	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	77	theme	apolipoprotein	567:580	arg1	ApoC-III					589:596	ApoC-III	589:596	ApoC-III	589:596	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	6	78	theme	A1AT	1097:1100	arg1	sites					1116:1120	multiple A1AT glycosylation sites	1088:1120	multiple A1AT glycosylation sites	1088:1120	HDL that increased IL-6 secretion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted in mono-sialylated ApoC-III (ApoC-III1).
28287093	1	79	theme	immunomodulatory	268:283	arg1	function					285:292	HDL's immunomodulatory function	262:292	HDL's immunomodulatory function	262:292	The goal of this pilot study was to determine whether HDL glycoprotein composition affects HDL's immunomodulatory function.
28287093	0	80	theme	Clinical	84:91	arg1	Groups					93:98	Clinical Groups	84:98	Clinical Groups	84:98	HDL Glycoprotein Composition and Site-Specific Glycosylation Differentiates Between Clinical Groups and Affects IL-6 Secretion in Lipopolysaccharide-Stimulated Monocytes.
28287093	3	81	theme	C-III	582:586	arg1	A2HSG					649:653	A2HSG	649:653	A2HSG	649:653	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	81	theme	C-III	582:586	arg1	Concentrations					445:458	Concentrations	445:458	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG)	445:654	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	81	theme	C-III	582:586	arg1	glycovariations					679:693	the site-specific glycovariations	661:693	the site-specific glycovariations of ApoC-III, A1AT, and A2HSG	661:722	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	82	theme	lipopolysaccharide	496:513	arg1	LBP					532:534	LBP	532:534	LBP	532:534	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	3	82	theme	lipopolysaccharide	496:513	arg1	protein					523:529	lipopolysaccharide binding protein	496:529	lipopolysaccharide binding protein (LBP)	496:535	Concentrations of HDL-bound serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), apolipoprotein A-I (ApoA-I), apolipoprotein C-III (ApoC-III), α-1-antitrypsin (A1AT), and α-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2HSG were measured.
28287093	2	83	with	controls	326:333	arg1	syndrome					369:376	metabolic syndrome	359:376	metabolic syndrome (MetS) (n = 13)	359:392	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	83	with	controls	326:333	arg1	MetS					379:382	MetS	379:382	MetS	379:382	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
28287093	2	83	with	controls	326:333	arg1	n = 13					386:391	n = 13	386:391	n = 13	386:391	HDL were purified from healthy controls (n = 13), subjects with metabolic syndrome (MetS) (n = 13), and diabetic hemodialysis (HD) patients (n = 24).
26296369	7	0	from	hPBMCs	1678:1683	arg1	production					1656:1665	reduced production	1648:1665	reduced production of IL-4 in hPBMCs	1648:1683	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	6	1	theme	CD69	1492:1495	arg1	silencing					1497:1505	CD69 silencing	1492:1505	CD69 silencing	1492:1505	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	1	2	theme	regulatory	285:294	arg1	cells					305:309	activated regulatory T (Treg) cells	275:309	activated regulatory T (Treg) cells	275:309	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	1	3	theme	T	296:296	arg1	cells					305:309	activated regulatory T (Treg) cells	275:309	activated regulatory T (Treg) cells	275:309	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	8	4	theme	Treg-cell	1797:1805	arg1	differentiation					1807:1821	Treg-cell differentiation	1797:1821	Treg-cell differentiation	1797:1821	Our results demonstrate the functional and mechanistic interplays between CD69 and S100A8/S100A9 in supporting Treg-cell differentiation.
26296369	6	5	theme	36.54	1471:1475	arg1	%					1476:1476	%	1476:1476	%	1476:1476	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	7	6	theme	key	1563:1565	arg1	decrease					1596:1603	∼ 3.28-fold decrease	1584:1603	∼ 3.28-fold decrease upon CD69 silencing	1584:1623	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	7	6	theme	key	1563:1565	arg1	TGF-β					1577:1581	key regulator TGF-β	1563:1581	key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05)	1563:1634	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	7	7	theme	reduced	1648:1654	arg1	production					1656:1665	reduced production	1648:1665	reduced production of IL-4 in hPBMCs	1648:1683	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	4	8	theme	competition	877:887	arg1	assay					889:893	in vitro binding and competition assay	856:893	assay	889:893	CD69 specifically associates with S100A8/S100A9 complex as confirmed by in vitro binding and competition assay, and the treatment of CD69 with peptide-N-glycosidase significantly abolishes such association.
26296369	5	9	theme	glycosylation	1043:1055	arg1	CD69					1094:1097	CD69	1094:1097	CD69	1094:1097	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	5	9	theme	glycosylation	1043:1055	arg1	site					1057:1060	the glycosylation site	1039:1060	the glycosylation site	1039:1060	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	1	10	theme	immune	226:231	arg1	homeostasis					233:243	immune homeostasis	226:243	immune homeostasis	226:243	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	2	11	theme	blood	532:536	arg1	hPBMCs					557:562	hPBMCs	557:562	hPBMCs	557:562	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	2	11	theme	blood	532:536	arg1	cells					550:554	human peripheral blood mononuclear cells	515:554	human peripheral blood mononuclear cells (hPBMCs) (P < 0.01)	515:574	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	2	11	theme	blood	532:536	arg1	<					568:568	P < 0.01	566:573	P < 0.01	566:573	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	7	12	dep	decrease	1596:1603	arg1	<					1628:1628	P < 0.05	1626:1633	P < 0.05	1626:1633	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	4	13	theme	in	856:857	arg1	binding					865:871	in vitro binding and competition assay	856:893	binding	865:871	CD69 specifically associates with S100A8/S100A9 complex as confirmed by in vitro binding and competition assay, and the treatment of CD69 with peptide-N-glycosidase significantly abolishes such association.
26296369	5	14	theme	glycomics	1009:1017	arg1	analysis					1019:1026	the glycomics analysis	1005:1026	the glycomics analysis	1005:1026	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	2	15	theme	cells	468:472	arg1	conversion					439:448	the conversion	435:448	the conversion of CD4(+) naive T cells into Treg cells	435:488	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	7	16	theme	P	1626:1626	arg1	<					1628:1628	P < 0.05	1626:1633	P < 0.05	1626:1633	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	2	17	theme	naive	460:464	arg1	cells					468:472	CD4(+) naive T cells	453:472	CD4(+) naive T cells	453:472	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	5	18	theme	cells	1220:1224	arg1	generation					1176:1185	the generation	1172:1185	the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05)	1172:1243	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	3	19	theme	CD69	768:771	arg1	complex					735:741	the S100A8/S100A9 complex	717:741	the S100A8/S100A9 complex	717:741	Using immunoprecipitation and mass spectrometry, we identified the S100A8/S100A9 complex as the natural ligand of CD69 in hPBMCs.
26296369	3	19	theme	CD69	768:771	arg1	ligand					758:763	the natural ligand	746:763	the natural ligand of CD69 in hPBMCs	746:781	Using immunoprecipitation and mass spectrometry, we identified the S100A8/S100A9 complex as the natural ligand of CD69 in hPBMCs.
26296369	0	20	theme	regulatory	91:100	arg1	differentiation					109:123	regulatory T-cell differentiation	91:123	regulatory T-cell differentiation	91:123	Glycosylation-dependent interaction between CD69 and S100A8/S100A9 complex is required for regulatory T-cell differentiation.
26296369	1	21	theme	Treg-cell	340:348	arg1	differentiation					350:364	Treg-cell differentiation	340:364	Treg-cell differentiation	340:364	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	1	22	theme	leukocyte	165:173	arg1	Cluster					126:132	Cluster	126:132	Cluster of differentiation (CD)69	126:158	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	1	22	theme	leukocyte	165:173	arg1	receptor					186:193	a leukocyte activation receptor	163:193	a leukocyte activation receptor involved in the maintenance of immune homeostasis	163:243	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	5	23	dep	%	1232:1232	arg1	<					1237:1237	P < 0.05	1235:1242	P < 0.05	1235:1242	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	2	24	theme	Treg	479:482	arg1	cells					484:488	Treg cells	479:488	Treg cells	479:488	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	2	25	theme	human	515:519	arg1	hPBMCs					557:562	hPBMCs	557:562	hPBMCs	557:562	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	2	25	theme	human	515:519	arg1	cells					550:554	human peripheral blood mononuclear cells	515:554	human peripheral blood mononuclear cells (hPBMCs) (P < 0.01)	515:574	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	2	25	theme	human	515:519	arg1	<					568:568	P < 0.01	566:573	P < 0.01	566:573	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	5	26	theme	N-glycan	1070:1077	arg1	composition					1079:1089	the N-glycan composition	1066:1089	the N-glycan composition of CD69	1066:1097	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	0	27	theme	S100A8/S100A9	53:65	arg1	complex					67:73	S100A8/S100A9 complex	53:73	S100A8/S100A9 complex	53:73	Glycosylation-dependent interaction between CD69 and S100A8/S100A9 complex is required for regulatory T-cell differentiation.
26296369	7	28	theme	3.28-fold	1586:1594	arg1	decrease					1596:1603	∼ 3.28-fold decrease	1584:1603	∼ 3.28-fold decrease upon CD69 silencing	1584:1623	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	7	28	theme	3.28-fold	1586:1594	arg1	TGF-β					1577:1581	key regulator TGF-β	1563:1581	key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05)	1563:1634	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	2	29	theme	ligand-receptor	595:609	arg1	interaction					611:621	a ligand-receptor interaction	593:621	a ligand-receptor interaction	593:621	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	0	30	theme	Glycosylation-dependent	0:22	arg1	interaction					24:34	Glycosylation-dependent interaction	0:34	Glycosylation-dependent interaction between CD69 and S100A8/S100A9 complex	0:73	Glycosylation-dependent interaction between CD69 and S100A8/S100A9 complex is required for regulatory T-cell differentiation.
26296369	5	31	theme	acid	1131:1134	arg1	removal					1113:1119	terminal removal	1104:1119	terminal removal of sialic acid from that N-linked glycans	1104:1161	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	4	32	theme	S100A8/S100A9	818:830	arg1	complex					832:838	S100A8/S100A9 complex	818:838	S100A8/S100A9 complex	818:838	CD69 specifically associates with S100A8/S100A9 complex as confirmed by in vitro binding and competition assay, and the treatment of CD69 with peptide-N-glycosidase significantly abolishes such association.
26296369	3	33	from	ligand	758:763	arg1	hPBMCs					776:781	hPBMCs	776:781	hPBMCs	776:781	Using immunoprecipitation and mass spectrometry, we identified the S100A8/S100A9 complex as the natural ligand of CD69 in hPBMCs.
26296369	6	34	theme	signaling	1371:1379	arg1	suppressor					1348:1357	suppressor	1348:1357	suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01)	1348:1516	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	1	35	theme	Treg	299:302	arg1	cells					305:309	activated regulatory T (Treg) cells	275:309	activated regulatory T (Treg) cells	275:309	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	7	36	theme	regulator	1567:1575	arg1	decrease					1596:1603	∼ 3.28-fold decrease	1584:1603	∼ 3.28-fold decrease upon CD69 silencing	1584:1623	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	7	36	theme	regulator	1567:1575	arg1	TGF-β					1577:1581	key regulator TGF-β	1563:1581	key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05)	1563:1634	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	5	37	theme	terminal	1104:1111	arg1	removal					1113:1119	terminal removal	1104:1119	terminal removal of sialic acid from that N-linked glycans	1104:1161	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	4	38	theme	such	973:976	arg1	association					978:988	such association	973:988	such association	973:988	CD69 specifically associates with S100A8/S100A9 complex as confirmed by in vitro binding and competition assay, and the treatment of CD69 with peptide-N-glycosidase significantly abolishes such association.
26296369	6	39	theme	activator	1441:1449	arg1	signaling					1406:1414	inhibited signaling	1396:1414	inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01)	1396:1516	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	1	40	theme	homeostasis	233:243	arg1	maintenance					211:221	the maintenance	207:221	the maintenance of immune homeostasis	207:243	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	6	41	theme	transducer	1426:1435	arg1	signaling					1406:1414	inhibited signaling	1396:1414	inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01)	1396:1516	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	5	42	theme	box	1199:1201	arg1	cells					1220:1224	forkhead box P3-positive Treg cells	1190:1224	forkhead box P3-positive Treg cells (23.21%; P < 0.05)	1190:1243	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	5	42	theme	box	1199:1201	arg1	%					1232:1232	23.21%	1227:1232	23.21%	1227:1232	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	2	43	theme	CD4	453:455	arg1	cells					468:472	CD4(+) naive T cells	453:472	CD4(+) naive T cells	453:472	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	6	44	theme	inhibited	1396:1404	arg1	signaling					1406:1414	inhibited signaling	1396:1414	inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01)	1396:1516	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	1	45	theme	activated	275:283	arg1	cells					305:309	activated regulatory T (Treg) cells	275:309	activated regulatory T (Treg) cells	275:309	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	2	46	theme	mononuclear	538:548	arg1	hPBMCs					557:562	hPBMCs	557:562	hPBMCs	557:562	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	2	46	theme	mononuclear	538:548	arg1	cells					550:554	human peripheral blood mononuclear cells	515:554	human peripheral blood mononuclear cells (hPBMCs) (P < 0.01)	515:574	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	2	46	theme	mononuclear	538:548	arg1	<					568:568	P < 0.01	566:573	P < 0.01	566:573	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	7	47	theme	IL-4	1670:1673	arg1	production					1656:1665	reduced production	1648:1665	reduced production of IL-4 in hPBMCs	1648:1683	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	1	48	theme	activation	175:184	arg1	Cluster					126:132	Cluster	126:132	Cluster of differentiation (CD)69	126:158	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	1	48	theme	activation	175:184	arg1	receptor					186:193	a leukocyte activation receptor	163:193	a leukocyte activation receptor involved in the maintenance of immune homeostasis	163:243	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	2	49	theme	P	566:566	arg1	cells					550:554	human peripheral blood mononuclear cells	515:554	human peripheral blood mononuclear cells (hPBMCs) (P < 0.01)	515:574	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	2	49	theme	P	566:566	arg1	<					568:568	P < 0.01	566:573	P < 0.01	566:573	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	6	50	dep	increase	1478:1485	arg1	<					1510:1510	P < 0.01	1508:1515	P < 0.01	1508:1515	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	6	51	theme	%	1476:1476	arg1	increase					1478:1485	36.54% increase	1471:1485	36.54% increase upon CD69 silencing	1471:1505	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	6	51	theme	%	1476:1476	arg1	transducer					1426:1435	signal transducer	1419:1435	signal transducer	1419:1435	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	3	52	theme	mass	684:687	arg1	spectrometry					689:700	mass spectrometry	684:700	mass spectrometry	684:700	Using immunoprecipitation and mass spectrometry, we identified the S100A8/S100A9 complex as the natural ligand of CD69 in hPBMCs.
26296369	5	53	from	glycans	1155:1161	arg1	removal					1113:1119	terminal removal	1104:1119	terminal removal of sialic acid from that N-linked glycans	1104:1161	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	6	54	theme	transcription	1454:1466	arg1	increase					1478:1485	36.54% increase	1471:1485	36.54% increase upon CD69 silencing	1471:1505	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	6	54	theme	transcription	1454:1466	arg1	activator					1441:1449	activator	1441:1449	activator	1441:1449	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	6	54	theme	transcription	1454:1466	arg1	transducer					1426:1435	signal transducer	1419:1435	signal transducer	1419:1435	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	2	55	theme	CD69	639:642	arg1	function					644:651	CD69 function	639:651	CD69 function	639:651	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	3	56	theme	S100A8/S100A9	721:733	arg1	complex					735:741	the S100A8/S100A9 complex	717:741	the S100A8/S100A9 complex	717:741	Using immunoprecipitation and mass spectrometry, we identified the S100A8/S100A9 complex as the natural ligand of CD69 in hPBMCs.
26296369	3	56	theme	S100A8/S100A9	721:733	arg1	ligand					758:763	the natural ligand	746:763	the natural ligand of CD69 in hPBMCs	746:781	Using immunoprecipitation and mass spectrometry, we identified the S100A8/S100A9 complex as the natural ligand of CD69 in hPBMCs.
26296369	1	57	theme	differentiation	137:151	arg1	CD					154:155	differentiation (CD)69	137:158	differentiation (CD)69	137:158	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	2	58	theme	peripheral	521:530	arg1	hPBMCs					557:562	hPBMCs	557:562	hPBMCs	557:562	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	2	58	theme	peripheral	521:530	arg1	cells					550:554	human peripheral blood mononuclear cells	515:554	human peripheral blood mononuclear cells (hPBMCs) (P < 0.01)	515:574	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	2	58	theme	peripheral	521:530	arg1	<					568:568	P < 0.01	566:573	P < 0.01	566:573	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	1	59	theme	CD	154:155	arg1	Cluster					126:132	Cluster	126:132	Cluster of differentiation (CD)69	126:158	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	1	59	theme	CD	154:155	arg1	receptor					186:193	a leukocyte activation receptor	163:193	a leukocyte activation receptor involved in the maintenance of immune homeostasis	163:243	Cluster of differentiation (CD)69 is a leukocyte activation receptor involved in the maintenance of immune homeostasis and is positively selected in activated regulatory T (Treg) cells, implicating its role during Treg-cell differentiation.
26296369	5	60	theme	P	1235:1235	arg1	<					1237:1237	P < 0.05	1235:1242	P < 0.05	1235:1242	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	3	61	theme	natural	750:756	arg1	complex					735:741	the S100A8/S100A9 complex	717:741	the S100A8/S100A9 complex	717:741	Using immunoprecipitation and mass spectrometry, we identified the S100A8/S100A9 complex as the natural ligand of CD69 in hPBMCs.
26296369	3	61	theme	natural	750:756	arg1	ligand					758:763	the natural ligand	746:763	the natural ligand of CD69 in hPBMCs	746:781	Using immunoprecipitation and mass spectrometry, we identified the S100A8/S100A9 complex as the natural ligand of CD69 in hPBMCs.
26296369	4	62	dep	in	856:857	arg1	vitro					859:863	vitro	859:863	vitro	859:863	CD69 specifically associates with S100A8/S100A9 complex as confirmed by in vitro binding and competition assay, and the treatment of CD69 with peptide-N-glycosidase significantly abolishes such association.
26296369	6	63	theme	P	1508:1508	arg1	<					1510:1510	P < 0.01	1508:1515	P < 0.01	1508:1515	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	2	64	theme	T	466:466	arg1	cells					468:472	CD4(+) naive T cells	453:472	CD4(+) naive T cells	453:472	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	5	65	theme	Treg	1215:1218	arg1	cells					1220:1224	forkhead box P3-positive Treg cells	1190:1224	forkhead box P3-positive Treg cells (23.21%; P < 0.05)	1190:1243	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	5	65	theme	Treg	1215:1218	arg1	%					1232:1232	23.21%	1227:1232	23.21%	1227:1232	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	0	66	theme	T-cell	102:107	arg1	differentiation					109:123	regulatory T-cell differentiation	91:123	regulatory T-cell differentiation	91:123	Glycosylation-dependent interaction between CD69 and S100A8/S100A9 complex is required for regulatory T-cell differentiation.
26296369	5	67	theme	CD69	1094:1097	arg1	CD69					1094:1097	CD69	1094:1097	CD69	1094:1097	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	5	67	theme	CD69	1094:1097	arg1	composition					1079:1089	the N-glycan composition	1066:1089	the N-glycan composition of CD69	1066:1097	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	5	67	theme	CD69	1094:1097	arg1	site					1057:1060	the glycosylation site	1039:1060	the glycosylation site	1039:1060	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	4	68	theme	CD69	917:920	arg1	treatment					904:912	the treatment	900:912	the treatment of CD69 with peptide-N-glycosidase	900:947	CD69 specifically associates with S100A8/S100A9 complex as confirmed by in vitro binding and competition assay, and the treatment of CD69 with peptide-N-glycosidase significantly abolishes such association.
26296369	6	69	theme	CD69-S100A8/S100A9	1280:1297	arg1	association					1299:1309	CD69-S100A8/S100A9 association	1280:1309	CD69-S100A8/S100A9 association	1280:1309	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	7	70	theme	CD69	1610:1613	arg1	silencing					1615:1623	CD69 silencing	1610:1623	CD69 silencing	1610:1623	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	8	71	theme	functional	1714:1723	arg1	interplays					1741:1750	the functional and mechanistic interplays	1710:1750	the functional and mechanistic interplays between CD69 and S100A8/S100A9 in supporting Treg-cell differentiation	1710:1821	Our results demonstrate the functional and mechanistic interplays between CD69 and S100A8/S100A9 in supporting Treg-cell differentiation.
26296369	8	72	theme	mechanistic	1729:1739	arg1	interplays					1741:1750	the functional and mechanistic interplays	1710:1750	the functional and mechanistic interplays between CD69 and S100A8/S100A9 in supporting Treg-cell differentiation	1710:1821	Our results demonstrate the functional and mechanistic interplays between CD69 and S100A8/S100A9 in supporting Treg-cell differentiation.
26296369	7	73	theme	∼	1584:1584	arg1	decrease					1596:1603	∼ 3.28-fold decrease	1584:1603	∼ 3.28-fold decrease upon CD69 silencing	1584:1623	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	7	73	theme	∼	1584:1584	arg1	TGF-β					1577:1581	key regulator TGF-β	1563:1581	key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05)	1563:1634	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	6	74	theme	cytokine	1362:1369	arg1	signaling					1371:1379	cytokine signaling 3	1362:1381	cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01)	1362:1516	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	5	75	theme	N-linked	1146:1153	arg1	glycans					1155:1161	that N-linked glycans	1141:1161	that N-linked glycans	1141:1161	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	7	76	theme	TGF-β	1577:1581	arg1	secretion					1550:1558	the secretion	1546:1558	the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05)	1546:1634	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	6	77	theme	suppressor	1348:1357	arg1	up-regulation					1331:1343	the up-regulation	1327:1343	the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01)	1327:1516	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	5	78	theme	sialic	1124:1129	arg1	acid					1131:1134	sialic acid	1124:1134	sialic acid	1124:1134	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	4	79	with	treatment	904:912	arg1	peptide-N-glycosidase					927:947	peptide-N-glycosidase	927:947	peptide-N-glycosidase	927:947	CD69 specifically associates with S100A8/S100A9 complex as confirmed by in vitro binding and competition assay, and the treatment of CD69 with peptide-N-glycosidase significantly abolishes such association.
26296369	7	80	from	production	1656:1665	arg1	hPBMCs					1678:1683	hPBMCs	1678:1683	hPBMCs	1678:1683	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	5	81	theme	forkhead	1190:1197	arg1	cells					1220:1224	forkhead box P3-positive Treg cells	1190:1224	forkhead box P3-positive Treg cells (23.21%; P < 0.05)	1190:1243	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	5	81	theme	forkhead	1190:1197	arg1	%					1232:1232	23.21%	1227:1232	23.21%	1227:1232	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	5	82	link	N-linked	1146:1153	arg1	glycans					1155:1161	that N-linked glycans	1141:1161	that N-linked glycans	1141:1161	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	7	83	from	IL-4	1670:1673	arg1	hPBMCs					1678:1683	hPBMCs	1678:1683	hPBMCs	1678:1683	This might in turn support the secretion of key regulator TGF-β (∼ 3.28-fold decrease upon CD69 silencing; P < 0.05), leading to reduced production of IL-4 in hPBMCs.
26296369	5	84	gly	glycosylation	1043:1055	arg2	site					1057:1060	the glycosylation site	1039:1060	the glycosylation site	1039:1060	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	5	84	gly	glycosylation	1043:1055	arg1	CD69					1094:1097	CD69	1094:1097	CD69	1094:1097	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	5	84	gly	glycosylation	1043:1055	arg2	CD69					1094:1097	CD69	1094:1097	CD69	1094:1097	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	2	85	theme	RNA	370:372	arg1	interference					374:385	RNA interference	370:385	RNA interference	370:385	By RNA interference, we show that CD69 is not sufficient to support the conversion of CD4(+) naive T cells into Treg cells, whereas it does that of human peripheral blood mononuclear cells (hPBMCs) (P < 0.01), suggesting that a ligand-receptor interaction is required for CD69 function.
26296369	6	86	theme	signal	1419:1424	arg1	increase					1478:1485	36.54% increase	1471:1485	36.54% increase upon CD69 silencing	1471:1505	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	6	86	theme	signal	1419:1424	arg1	transducer					1426:1435	signal transducer	1419:1435	signal transducer	1419:1435	More specifically, we showed that CD69-S100A8/S100A9 association is required for the up-regulation of suppressor of cytokine signaling 3 resulting in inhibited signaling of signal transducer and activator of transcription 3 (36.54% increase upon CD69 silencing; P < 0.01).
26296369	5	87	theme	P3-positive	1203:1213	arg1	cells					1220:1224	forkhead box P3-positive Treg cells	1190:1224	forkhead box P3-positive Treg cells (23.21%; P < 0.05)	1190:1243	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
26296369	5	87	theme	P3-positive	1203:1213	arg1	%					1232:1232	23.21%	1227:1232	23.21%	1227:1232	In agreement, the glycomics analysis determines the glycosylation site and the N-glycan composition of CD69, and terminal removal of sialic acid from that N-linked glycans reverses the generation of forkhead box P3-positive Treg cells (23.21%; P < 0.05).
28396675	8	0	theme	spectrometry	1412:1423	arg1	analysis					1425:1432	mass spectrometry analysis	1407:1432	mass spectrometry analysis of the extracellular proteins	1407:1462	The absence of these carbohydrates was confirmed by mass spectrometry analysis of the extracellular proteins.
28396675	0	1	theme	CRISPR/Cas9	133:143	arg1	Strategy					145:152	a Multiplex CRISPR/Cas9 Strategy	121:152	a Multiplex CRISPR/Cas9 Strategy	121:152	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	4	2	theme	tabacum	762:768	arg1	cells					786:790	Nicotiana tabacum BY-2 suspension cells	752:790	Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9	752:808	Our aim consisted of knocking-out two XylT genes and four FucT genes (12 alleles altogether) in Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9.
28396675	7	3	theme	-xylose	1241:1247	arg1	-fucose					1259:1265	β(1,2)-xylose and α(1,3)-fucose	1235:1265	β(1,2)-xylose and α(1,3)-fucose	1235:1265	Three lines showed a strong reduction of β(1,2)-xylose and α(1,3)-fucose, while two lines were completely devoid of them, indicating complete gene inactivation.
28396675	14	4	theme	pharmacological	2125:2139	arg1	proteins					2141:2148	pharmacological proteins	2125:2148	pharmacological proteins expressed in N. tabacum BY-2 cells	2125:2183	These data represent an important step toward humanizing the glycosylation of pharmacological proteins expressed in N. tabacum BY-2 cells.
28396675	13	5	theme	-xylose	1925:1931	arg1	-fucose					1942:1948	no β(1,2)-xylose or α(1,3)-fucose	1916:1948	no β(1,2)-xylose or α(1,3)-fucose	1916:1948	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	9	6	theme	PCR	1465:1467	arg1	amplification					1469:1481	PCR amplification	1465:1481	PCR amplification	1465:1481	PCR amplification and sequencing of the targeted region indicated small INDEL and/or deletions between the target sites.
28396675	7	7	theme	1,2	1237:1239	arg1	-xylose					1241:1247	β(1,2)-xylose	1235:1247	β(1,2)-xylose	1235:1247	Three lines showed a strong reduction of β(1,2)-xylose and α(1,3)-fucose, while two lines were completely devoid of them, indicating complete gene inactivation.
28396675	6	8	theme	Western	1115:1121	arg1	blotting					1123:1130	Western blotting	1115:1130	Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose	1115:1191	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	12	9	from	high	1769:1772	arg1	transformant					1790:1801	a control transformant	1780:1801	a control transformant which had not been glycoengineered	1780:1836	The IgG2 expression level was as high as in a control transformant which had not been glycoengineered.
28396675	7	10	theme	β	1235:1235	arg1	-xylose					1241:1247	β(1,2)-xylose	1235:1247	β(1,2)-xylose	1235:1247	Three lines showed a strong reduction of β(1,2)-xylose and α(1,3)-fucose, while two lines were completely devoid of them, indicating complete gene inactivation.
28396675	9	11	theme	small	1531:1535	arg1	INDEL					1537:1541	small INDEL	1531:1541	small INDEL	1531:1541	PCR amplification and sequencing of the targeted region indicated small INDEL and/or deletions between the target sites.
28396675	12	12	from	transformant	1790:1801	arg1	level					1756:1760	The IgG2 expression level	1736:1760	The IgG2 expression level	1736:1760	The IgG2 expression level was as high as in a control transformant which had not been glycoengineered.
28396675	12	12	from	transformant	1790:1801	arg1	high					1769:1772	high	1769:1772	high	1769:1772	The IgG2 expression level was as high as in a control transformant which had not been glycoengineered.
28396675	0	13	theme	Plant-Specific	187:200	arg1	Glycans					202:208	Plant-Specific Glycans	187:208	Plant-Specific Glycans	187:208	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	9	14	theme	targeted	1505:1512	arg1	region					1514:1519	the targeted region	1501:1519	the targeted region	1501:1519	PCR amplification and sequencing of the targeted region indicated small INDEL and/or deletions between the target sites.
28396675	0	15	theme	tabacum	99:105	arg1	Cells					112:116	Nicotiana tabacum BY-2 Cells	89:116	Nicotiana tabacum BY-2 Cells	89:116	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	12	16	theme	IgG2	1740:1743	arg1	level					1756:1760	The IgG2 expression level	1736:1760	The IgG2 expression level	1736:1760	The IgG2 expression level was as high as in a control transformant which had not been glycoengineered.
28396675	12	16	theme	IgG2	1740:1743	arg1	high					1769:1772	high	1769:1772	high	1769:1772	The IgG2 expression level was as high as in a control transformant which had not been glycoengineered.
28396675	4	17	theme	suspension	775:784	arg1	cells					786:790	Nicotiana tabacum BY-2 suspension cells	752:790	Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9	752:808	Our aim consisted of knocking-out two XylT genes and four FucT genes (12 alleles altogether) in Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9.
28396675	13	18	dep	α	1936:1936	arg1	1,3					1938:1940	1,3	1938:1940	1,3	1938:1940	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	6	19	theme	protein	1078:1084	arg1	complements					1086:1096	their extracellular as well as intracellular protein complements	1033:1096	their extracellular as well as intracellular protein complements	1033:1096	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	0	20	theme	Multiplex	123:131	arg1	Strategy					145:152	a Multiplex CRISPR/Cas9 Strategy	121:152	a Multiplex CRISPR/Cas9 Strategy	121:152	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	1	21	gly	glycoproteins	272:284	arg1	vaccines					308:315	vaccines	308:315	vaccines	308:315	Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines.
28396675	1	21	gly	glycoproteins	272:284	arg1	glycoproteins					272:284	pharmacological glycoproteins	256:284	pharmacological glycoproteins such as antibodies or vaccines	256:315	Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines.
28396675	1	21	gly	glycoproteins	272:284	arg1	antibodies					294:303	antibodies	294:303	antibodies	294:303	Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines.
28396675	2	22	theme	protein	504:510	arg1	activity					488:495	activity	488:495	activity	488:495	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	2	22	theme	protein	504:510	arg1	immunogenicity					454:467	immunogenicity	454:467	immunogenicity	454:467	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	2	22	theme	protein	504:510	arg1	allergenicity					470:482	allergenicity	470:482	allergenicity	470:482	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	14	23	theme	important	2071:2079	arg1	step					2081:2084	an important step	2068:2084	an important step toward humanizing the glycosylation of pharmacological proteins expressed in N. tabacum BY-2 cells	2068:2183	These data represent an important step toward humanizing the glycosylation of pharmacological proteins expressed in N. tabacum BY-2 cells.
28396675	13	24	theme	mass	1883:1886	arg1	spectrometry					1888:1899	mass spectrometry	1883:1899	mass spectrometry	1883:1899	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	3	25	theme	plant-specific	561:574	arg1	glycans					576:582	plant-specific glycans	561:582	plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT)	561:653	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	25	theme	plant-specific	561:574	arg1	XylT					612:615	β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	585:646	XylT	612:615	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	25	theme	plant-specific	561:574	arg1	-fucosyltransferase					628:646	β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	585:646	-fucosyltransferase	628:646	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	13	26	theme	GnGn	2031:2034	arg1	structure					2036:2044	the GnGn structure	2027:2044	the GnGn structure	2027:2044	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	13	26	theme	GnGn	2031:2034	arg1	glycoform					2013:2021	the dominant glycoform	2000:2021	the dominant glycoform	2000:2021	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	6	27	theme	N.	909:910	arg1	tabacum					912:918	N. tabacum	909:918	N. tabacum BY-2 cells	909:929	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	13	28	located	present	1955:1961	arg2	-fucose					1942:1948	no β(1,2)-xylose or α(1,3)-fucose	1916:1948	no β(1,2)-xylose or α(1,3)-fucose	1916:1948	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	13	28	located	present	1955:1961	arg1	moiety					1984:1989	the glycosylation moiety	1966:1989	the glycosylation moiety	1966:1989	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	13	29	theme	glycosylation	1970:1982	arg1	moiety					1984:1989	the glycosylation moiety	1966:1989	the glycosylation moiety	1966:1989	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	0	30	theme	α	54:54	arg1	genes					80:84	the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes	16:84	the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells	16:116	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	13	31	theme	glycosylation	1847:1859	arg1	profile					1861:1867	The IgG glycosylation profile	1839:1867	The IgG glycosylation profile determined by mass spectrometry	1839:1899	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	13	32	theme	dominant	2004:2011	arg1	structure					2036:2044	the GnGn structure	2027:2044	the GnGn structure	2027:2044	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	13	32	theme	dominant	2004:2011	arg1	glycoform					2013:2021	the dominant glycoform	2000:2021	the dominant glycoform	2000:2021	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	0	33	from	Inactivation	0:11	arg1	Cells					112:116	Nicotiana tabacum BY-2 Cells	89:116	Nicotiana tabacum BY-2 Cells	89:116	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	7	34	theme	gene	1336:1339	arg1	inactivation					1341:1352	complete gene inactivation	1327:1352	complete gene inactivation	1327:1352	Three lines showed a strong reduction of β(1,2)-xylose and α(1,3)-fucose, while two lines were completely devoid of them, indicating complete gene inactivation.
28396675	0	35	theme	-fucosyltransferase	60:78	arg1	genes					80:84	the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes	16:84	the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells	16:116	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	11	36	theme	human	1715:1719	arg1	antibody					1726:1733	a human IgG2 antibody	1713:1733	a human IgG2 antibody	1713:1733	One KO line was transformed with genes encoding a human IgG2 antibody.
28396675	1	37	theme	pharmacological	256:270	arg1	vaccines					308:315	vaccines	308:315	vaccines	308:315	Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines.
28396675	1	37	theme	pharmacological	256:270	arg1	glycoproteins					272:284	pharmacological glycoproteins	256:284	pharmacological glycoproteins such as antibodies or vaccines	256:315	Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines.
28396675	1	37	theme	pharmacological	256:270	arg1	antibodies					294:303	antibodies	294:303	antibodies	294:303	Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines.
28396675	1	38	used	used	240:243	arg2	cells					227:231	plant cells	221:231	plant cells	221:231	Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines.
28396675	1	38	used	used	240:243	arg2	Plants					211:216	Plants	211:216	Plants	211:216	Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines.
28396675	6	39	theme	BY-2	920:923	arg1	cells					925:929	N. tabacum BY-2 cells	909:929	N. tabacum BY-2 cells	909:929	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	2	40	dep	residues	376:383	arg1	-xylose					392:398	[β(1,2)-xylose	385:398	[β(1,2)-xylose	385:398	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	2	40	dep	residues	376:383	arg1	α					409:409	core α	404:409	core α	404:409	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	3	41	dep	glycans	576:582	arg1	XylT					612:615	β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	585:646	XylT	612:615	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	41	dep	glycans	576:582	arg1	FucT					649:652	FucT	649:652	FucT	649:652	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	41	dep	glycans	576:582	arg1	glycans					576:582	plant-specific glycans	561:582	plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT)	561:653	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	41	dep	glycans	576:582	arg1	-fucosyltransferase					628:646	β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	585:646	-fucosyltransferase	628:646	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	6	42	theme	selectable	973:982	arg1	marker					984:989	a selectable marker	971:989	a selectable marker (bar)	971:995	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	6	42	theme	selectable	973:982	arg1	bar					992:994	bar	992:994	bar	992:994	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	8	43	theme	carbohydrates	1376:1388	arg1	absence					1359:1365	The absence	1355:1365	The absence of these carbohydrates	1355:1388	The absence of these carbohydrates was confirmed by mass spectrometry analysis of the extracellular proteins.
28396675	14	44	theme	tabacum	2166:2172	arg1	cells					2179:2183	N. tabacum BY-2 cells	2163:2183	N. tabacum BY-2 cells	2163:2183	These data represent an important step toward humanizing the glycosylation of pharmacological proteins expressed in N. tabacum BY-2 cells.
28396675	11	45	theme	KO	1669:1670	arg1	line					1672:1675	One KO line	1665:1675	One KO line	1665:1675	One KO line was transformed with genes encoding a human IgG2 antibody.
28396675	8	46	theme	proteins	1455:1462	arg1	analysis					1425:1432	mass spectrometry analysis	1407:1432	mass spectrometry analysis of the extracellular proteins	1407:1462	The absence of these carbohydrates was confirmed by mass spectrometry analysis of the extracellular proteins.
28396675	12	47	theme	control	1782:1788	arg1	transformant					1790:1801	a control transformant	1780:1801	a control transformant which had not been glycoengineered	1780:1836	The IgG2 expression level was as high as in a control transformant which had not been glycoengineered.
28396675	3	48	theme	α	622:622	arg1	glycans					576:582	plant-specific glycans	561:582	plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT)	561:653	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	48	theme	α	622:622	arg1	-fucosyltransferase					628:646	β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	585:646	-fucosyltransferase	628:646	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	6	49	theme	β	1161:1161	arg1	-xylose					1167:1173	β(1,2)-xylose	1161:1173	β(1,2)-xylose	1161:1173	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	7	50	theme	-fucose	1259:1265	arg1	reduction					1222:1230	a strong reduction	1213:1230	a strong reduction of β(1,2)-xylose and α(1,3)-fucose	1213:1265	Three lines showed a strong reduction of β(1,2)-xylose and α(1,3)-fucose, while two lines were completely devoid of them, indicating complete gene inactivation.
28396675	8	51	theme	mass	1407:1410	arg1	analysis					1425:1432	mass spectrometry analysis	1407:1432	mass spectrometry analysis of the extracellular proteins	1407:1462	The absence of these carbohydrates was confirmed by mass spectrometry analysis of the extracellular proteins.
28396675	14	52	theme	proteins	2141:2148	arg1	glycosylation					2108:2120	the glycosylation	2104:2120	the glycosylation of pharmacological proteins expressed in N. tabacum BY-2 cells	2104:2183	These data represent an important step toward humanizing the glycosylation of pharmacological proteins expressed in N. tabacum BY-2 cells.
28396675	4	53	theme	Nicotiana	752:760	arg1	tabacum					762:768	Nicotiana tabacum	752:768	Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9	752:808	Our aim consisted of knocking-out two XylT genes and four FucT genes (12 alleles altogether) in Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9.
28396675	0	54	from	Cells	112:116	arg1	Inactivation					0:11	Inactivation	0:11	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy	0:152	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	7	55	theme	α	1253:1253	arg1	-fucose					1259:1265	β(1,2)-xylose and α(1,3)-fucose	1235:1265	β(1,2)-xylose and α(1,3)-fucose	1235:1265	Three lines showed a strong reduction of β(1,2)-xylose and α(1,3)-fucose, while two lines were completely devoid of them, indicating complete gene inactivation.
28396675	3	56	theme	1,3	624:626	arg1	glycans					576:582	plant-specific glycans	561:582	plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT)	561:653	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	56	theme	1,3	624:626	arg1	-fucosyltransferase					628:646	β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	585:646	-fucosyltransferase	628:646	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	10	57	theme	KO	1590:1591	arg1	lines					1593:1597	The KO lines	1586:1597	The KO lines	1586:1597	The KO lines did not show any particular morphology and grew as the wild-type.
28396675	12	58	theme	expression	1745:1754	arg1	level					1756:1760	The IgG2 expression level	1736:1760	The IgG2 expression level	1736:1760	The IgG2 expression level was as high as in a control transformant which had not been glycoengineered.
28396675	12	58	theme	expression	1745:1754	arg1	high					1769:1772	high	1769:1772	high	1769:1772	The IgG2 expression level was as high as in a control transformant which had not been glycoengineered.
28396675	6	59	theme	transgenic	998:1007	arg1	lines					1009:1013	transgenic lines	998:1013	transgenic lines	998:1013	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	0	60	theme	Nicotiana	89:97	arg1	tabacum					99:105	Nicotiana tabacum	89:105	Nicotiana tabacum BY-2 Cells	89:116	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	4	61	dep	genes	719:723	arg1	alleles					729:735	12 alleles	726:735	12 alleles altogether	726:746	Our aim consisted of knocking-out two XylT genes and four FucT genes (12 alleles altogether) in Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9.
28396675	6	62	theme	α	1179:1179	arg1	-fucose					1185:1191	α(1,3)-fucose	1179:1191	α(1,3)-fucose	1179:1191	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	9	63	theme	region	1514:1519	arg1	amplification					1469:1481	PCR amplification	1465:1481	PCR amplification	1465:1481	PCR amplification and sequencing of the targeted region indicated small INDEL and/or deletions between the target sites.
28396675	9	63	theme	region	1514:1519	arg1	sequencing					1487:1496	sequencing	1487:1496	sequencing	1487:1496	PCR amplification and sequencing of the targeted region indicated small INDEL and/or deletions between the target sites.
28396675	6	64	with	transformation	891:904	arg1	genes					936:940	genes	936:940	genes coding for sgRNAs, Cas9, and a selectable marker (bar)	936:995	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	4	65	theme	BY-2	770:773	arg1	cells					786:790	Nicotiana tabacum BY-2 suspension cells	752:790	Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9	752:808	Our aim consisted of knocking-out two XylT genes and four FucT genes (12 alleles altogether) in Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9.
28396675	0	66	from	genes	80:84	arg1	Cells					112:116	Nicotiana tabacum BY-2 Cells	89:116	Nicotiana tabacum BY-2 Cells	89:116	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	6	67	theme	intracellular	1064:1076	arg1	complements					1086:1096	their extracellular as well as intracellular protein complements	1033:1096	their extracellular as well as intracellular protein complements	1033:1096	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	6	68	theme	1,2	1163:1165	arg1	-xylose					1167:1173	β(1,2)-xylose	1161:1173	β(1,2)-xylose	1161:1173	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	6	69	theme	tabacum	912:918	arg1	cells					925:929	N. tabacum BY-2 cells	909:929	N. tabacum BY-2 cells	909:929	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	10	70	theme	particular	1616:1625	arg1	morphology					1627:1636	any particular morphology	1612:1636	any particular morphology	1612:1636	The KO lines did not show any particular morphology and grew as the wild-type.
28396675	6	71	theme	extracellular	1039:1051	arg1	complements					1086:1096	their extracellular as well as intracellular protein complements	1033:1096	their extracellular as well as intracellular protein complements	1033:1096	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	13	72	theme	IgG	1843:1845	arg1	profile					1861:1867	The IgG glycosylation profile	1839:1867	The IgG glycosylation profile determined by mass spectrometry	1839:1899	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	2	73	dep	-fucose	415:421	arg1	1,3					411:413	1,3	411:413	1,3	411:413	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	9	74	theme	target	1572:1577	arg1	sites					1579:1583	the target sites	1568:1583	the target sites	1568:1583	PCR amplification and sequencing of the targeted region indicated small INDEL and/or deletions between the target sites.
28396675	7	75	theme	complete	1327:1334	arg1	inactivation					1341:1352	complete gene inactivation	1327:1352	complete gene inactivation	1327:1352	Three lines showed a strong reduction of β(1,2)-xylose and α(1,3)-fucose, while two lines were completely devoid of them, indicating complete gene inactivation.
28396675	0	76	theme	genes	80:84	arg1	Inactivation					0:11	Inactivation	0:11	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy	0:152	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	2	77	contain	carry	341:345	arg2	N-glycans					347:355	N-glycans	347:355	N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein	347:510	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	2	77	contain	carry	341:345	arg1	proteins					332:339	these proteins	326:339	these proteins	326:339	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	2	78	theme	[β	385:386	arg1	-xylose					392:398	[β(1,2)-xylose	385:398	[β(1,2)-xylose	385:398	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	0	79	theme	BY-2	107:110	arg1	Cells					112:116	Nicotiana tabacum BY-2 Cells	89:116	Nicotiana tabacum BY-2 Cells	89:116	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	2	80	theme	plant-typical	362:374	arg1	-fucose					415:421	1,3)-fucose	411:421	1,3)-fucose	411:421	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	2	80	theme	plant-typical	362:374	arg1	residues					376:383	plant-typical residues	362:383	plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose]	362:422	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	0	81	theme	β	20:20	arg1	-xylosyltransferase					26:44	β(1,2)-xylosyltransferase	20:44	β(1,2)-xylosyltransferase	20:44	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	2	82	dep	immunogenicity	454:467	arg1	the					450:452	the	450:452	the	450:452	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	5	83	theme	FucT	830:833	arg1	sgRNAs					835:840	Three XylT and six FucT sgRNAs	811:840	sgRNAs	835:840	Three XylT and six FucT sgRNAs were designed to target conserved regions.
28396675	2	84	theme	core	404:407	arg1	α					409:409	core α	404:409	core α	404:409	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	3	85	theme	β	585:585	arg1	XylT					612:615	β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	585:646	XylT	612:615	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	85	theme	β	585:585	arg1	FucT					649:652	FucT	649:652	FucT	649:652	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	85	theme	β	585:585	arg1	glycans					576:582	plant-specific glycans	561:582	plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT)	561:653	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	11	86	theme	IgG2	1721:1724	arg1	antibody					1726:1733	a human IgG2 antibody	1713:1733	a human IgG2 antibody	1713:1733	One KO line was transformed with genes encoding a human IgG2 antibody.
28396675	0	87	theme	1,2	22:24	arg1	-xylosyltransferase					26:44	β(1,2)-xylosyltransferase	20:44	β(1,2)-xylosyltransferase	20:44	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	14	88	gly	glycosylation	2108:2120	arg1	proteins					2141:2148	pharmacological proteins	2125:2148	pharmacological proteins expressed in N. tabacum BY-2 cells	2125:2183	These data represent an important step toward humanizing the glycosylation of pharmacological proteins expressed in N. tabacum BY-2 cells.
28396675	6	89	theme	cells	925:929	arg1	transformation					891:904	transformation	891:904	transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar)	891:995	After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose.
28396675	3	90	theme	glycans	576:582	arg1	addition					549:556	the addition	545:556	the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT)	545:653	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	2	91	theme	1,2	388:390	arg1	-xylose					392:398	[β(1,2)-xylose	385:398	[β(1,2)-xylose	385:398	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	0	92	theme	-xylosyltransferase	26:44	arg1	genes					80:84	the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes	16:84	the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells	16:116	Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans.
28396675	7	93	theme	them	1310:1313	arg1	devoid					1300:1305	devoid	1300:1305	devoid	1300:1305	Three lines showed a strong reduction of β(1,2)-xylose and α(1,3)-fucose, while two lines were completely devoid of them, indicating complete gene inactivation.
28396675	3	94	theme	-xylosyltransferase	591:609	arg1	XylT					612:615	β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	585:646	XylT	612:615	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	94	theme	-xylosyltransferase	591:609	arg1	FucT					649:652	FucT	649:652	FucT	649:652	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	94	theme	-xylosyltransferase	591:609	arg1	glycans					576:582	plant-specific glycans	561:582	plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT)	561:653	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	4	95	theme	FucT	714:717	arg1	genes					719:723	four FucT genes	709:723	four FucT genes (12 alleles altogether)	709:747	Our aim consisted of knocking-out two XylT genes and four FucT genes (12 alleles altogether) in Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9.
28396675	8	96	theme	extracellular	1441:1453	arg1	proteins					1455:1462	the extracellular proteins	1437:1462	the extracellular proteins	1437:1462	The absence of these carbohydrates was confirmed by mass spectrometry analysis of the extracellular proteins.
28396675	5	97	theme	conserved	866:874	arg1	regions					876:882	conserved regions	866:882	conserved regions	866:882	Three XylT and six FucT sgRNAs were designed to target conserved regions.
28396675	7	98	theme	strong	1215:1220	arg1	reduction					1222:1230	a strong reduction	1213:1230	a strong reduction of β(1,2)-xylose and α(1,3)-fucose	1213:1265	Three lines showed a strong reduction of β(1,2)-xylose and α(1,3)-fucose, while two lines were completely devoid of them, indicating complete gene inactivation.
28396675	14	99	theme	N.	2163:2164	arg1	tabacum					2166:2172	N. tabacum	2163:2172	N. tabacum BY-2 cells	2163:2183	These data represent an important step toward humanizing the glycosylation of pharmacological proteins expressed in N. tabacum BY-2 cells.
28396675	3	100	theme	1,2	587:589	arg1	XylT					612:615	β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	585:646	XylT	612:615	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	100	theme	1,2	587:589	arg1	FucT					649:652	FucT	649:652	FucT	649:652	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	3	100	theme	1,2	587:589	arg1	glycans					576:582	plant-specific glycans	561:582	plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT)	561:653	Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT).
28396675	4	101	theme	knocking-out	677:688	arg1	genes					699:703	knocking-out two XylT genes	677:703	knocking-out two XylT genes	677:703	Our aim consisted of knocking-out two XylT genes and four FucT genes (12 alleles altogether) in Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9.
28396675	14	102	theme	BY-2	2174:2177	arg1	cells					2179:2183	N. tabacum BY-2 cells	2163:2183	N. tabacum BY-2 cells	2163:2183	These data represent an important step toward humanizing the glycosylation of pharmacological proteins expressed in N. tabacum BY-2 cells.
28396675	13	103	theme	α	1936:1936	arg1	-fucose					1942:1948	no β(1,2)-xylose or α(1,3)-fucose	1916:1948	no β(1,2)-xylose or α(1,3)-fucose	1916:1948	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	2	104	with	N-glycans	347:355	arg1	-fucose					415:421	1,3)-fucose	411:421	1,3)-fucose	411:421	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	2	104	with	N-glycans	347:355	arg1	residues					376:383	plant-typical residues	362:383	plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose]	362:422	However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein.
28396675	4	105	theme	XylT	694:697	arg1	genes					699:703	knocking-out two XylT genes	677:703	knocking-out two XylT genes	677:703	Our aim consisted of knocking-out two XylT genes and four FucT genes (12 alleles altogether) in Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9.
28396675	13	106	attach	present	1955:1961	arg2	-fucose					1942:1948	no β(1,2)-xylose or α(1,3)-fucose	1916:1948	no β(1,2)-xylose or α(1,3)-fucose	1916:1948	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	13	106	attach	present	1955:1961	arg1	moiety					1984:1989	the glycosylation moiety	1966:1989	the glycosylation moiety	1966:1989	The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure.
28396675	1	107	theme	plant	221:225	arg1	cells					227:231	plant cells	221:231	plant cells	221:231	Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines.
24269369	14	0	theme	proteins	2165:2172	arg1	family					2118:2123	ADAM/ADAMTS family	2106:2123	ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential	2106:2197	VaH4 is also significant medically as we found it cytotoxic against cancer cells and due to its substantial sequence similarity to ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential.
24269369	5	1	theme	characterization	651:666	arg1	characterization					617:632	Complete structural characterization	597:632	Complete structural characterization of A and partial characterization of B	597:671	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	14	2	theme	important	2149:2157	arg1	proteins					2165:2172	physiologically very important human proteins	2128:2172	physiologically very important human proteins of therapeutic potential	2128:2197	VaH4 is also significant medically as we found it cytotoxic against cancer cells and due to its substantial sequence similarity to ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential.
24269369	5	3	theme	B	671:671	arg1	A					637:637	A	637:637	A	637:637	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	5	3	theme	B	671:671	arg1	characterization					651:666	partial characterization	643:666	partial characterization	643:666	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	13	4	theme	coagulation	1912:1922	arg1	proteins					1924:1931	some blood coagulation proteins	1901:1931	some blood coagulation proteins	1901:1931	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	12	5	gly	N-glycosylation	1555:1569	arg2	positions					1623:1631	completely different positions	1602:1631	completely different positions from those in homodimeric P-IIIc VaH3 from the same venom	1602:1689	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	12	5	gly	N-glycosylation	1555:1569	arg2	sites					1571:1575	The two possible N-glycosylation sites	1538:1575	The two possible N-glycosylation sites in VaH4-A	1538:1585	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	12	5	gly	N-glycosylation	1555:1569	arg2	two					1542:1544	two	1542:1544	two	1542:1544	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	12	5	gly	N-glycosylation	1555:1569	arg2	located					1591:1597	located	1591:1597	located	1591:1597	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	8	6	theme	Cys174	1148:1153	arg1	residues					1155:1162	their Cys132 or Cys174 residues	1132:1162	residues	1155:1162	This implies that dimers in the P-III class of SVMPs can be formed either between their Cys132 or Cys174 residues.
24269369	12	7	theme	evident	1713:1719	arg1	implications					1732:1743	any evident functional implications	1709:1743	any evident functional implications	1709:1743	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	13	8	theme	hemorrhagic	1750:1760	arg1	activity					1762:1769	The hemorrhagic activity	1746:1769	The hemorrhagic activity of this slightly acidic SVMP	1746:1798	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	1	9	from	consequence	278:288	arg1	man					293:295	man	293:295	man	293:295	In the envenomation caused by a bite of Vipera ammodytes ammodytes, the most venomous snake in Europe, hemorrhage is usually the most severe consequence in man.
24269369	1	9	from	consequence	278:288	arg1	envenomation					144:155	the envenomation	140:155	the envenomation caused by a bite of Vipera ammodytes ammodytes	140:202	In the envenomation caused by a bite of Vipera ammodytes ammodytes, the most venomous snake in Europe, hemorrhage is usually the most severe consequence in man.
24269369	0	10	theme	Hemorrhagin	0:10	arg1	VaH4					12:15	Hemorrhagin VaH4	0:15	Hemorrhagin VaH4	0:15	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	0	10	theme	Hemorrhagin	0:10	arg1	metalloproteinase					49:65	a covalent heterodimeric P-III metalloproteinase	18:65	a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity	18:134	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	9	11	with	interaction	1311:1321	arg1	element					1347:1353	the latter element	1336:1353	the latter element of the extracellular matrix (ECM)	1336:1387	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	12	12	theme	possible	1546:1553	arg1	located					1591:1597	located	1591:1597	located	1591:1597	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	12	12	theme	possible	1546:1553	arg1	sites					1571:1575	The two possible N-glycosylation sites	1538:1575	The two possible N-glycosylation sites in VaH4-A	1538:1585	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	9	13	theme	VaH4	1207:1210	arg1	stability					1194:1202	stability	1194:1202	stability	1194:1202	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	9	13	theme	VaH4	1207:1210	arg1	activity					1181:1188	proteolytic activity	1169:1188	proteolytic activity	1169:1188	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	5	14	theme	cysteine-rich	825:837	arg1	domain					839:844	a cysteine-rich domain	823:844	a cysteine-rich domain	823:844	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	5	14	theme	cysteine-rich	825:837	arg1	metalloproteinases					733:750	snake venom metalloproteinases	721:750	snake venom metalloproteinases (SVMPs)	721:758	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	6	15	theme	P-III	950:954	arg1	SVMPs					956:960	P-III SVMPs	950:960	P-III SVMPs	950:960	However, neither VaH4-A nor VaH4-B possess the Cys174 involved in the inter-subunit disulphide bond of P-III SVMPs.
24269369	6	16	contain	possess	882:888	arg1	VaH4-B					875:880	VaH4-B	875:880	VaH4-B	875:880	However, neither VaH4-A nor VaH4-B possess the Cys174 involved in the inter-subunit disulphide bond of P-III SVMPs.
24269369	6	16	contain	possess	882:888	arg2	Cys174					894:899	the Cys174	890:899	the Cys174 involved in the inter-subunit disulphide bond of P-III SVMPs	890:960	However, neither VaH4-A nor VaH4-B possess the Cys174 involved in the inter-subunit disulphide bond of P-III SVMPs.
24269369	6	16	contain	possess	882:888	arg1	VaH4-A					864:869	VaH4-A	864:869	VaH4-A	864:869	However, neither VaH4-A nor VaH4-B possess the Cys174 involved in the inter-subunit disulphide bond of P-III SVMPs.
24269369	14	17	theme	sequence	2083:2090	arg1	similarity					2092:2101	its substantial sequence similarity	2067:2101	its substantial sequence similarity to ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential	2067:2197	VaH4 is also significant medically as we found it cytotoxic against cancer cells and due to its substantial sequence similarity to ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential.
24269369	7	18	theme	VaH4	996:999	arg1	dimer					1001:1005	the VaH4 dimer	992:1005	the VaH4 dimer	992:1005	A three-dimensional model of the VaH4 dimer suggests that Cys132 serves this function.
24269369	6	19	theme	inter-subunit	917:929	arg1	bond					942:945	the inter-subunit disulphide bond	913:945	the inter-subunit disulphide bond of P-III SVMPs	913:960	However, neither VaH4-A nor VaH4-B possess the Cys174 involved in the inter-subunit disulphide bond of P-III SVMPs.
24269369	1	20	from	snake	223:227	arg1	hemorrhage					240:249	hemorrhage	240:249	hemorrhage	240:249	In the envenomation caused by a bite of Vipera ammodytes ammodytes, the most venomous snake in Europe, hemorrhage is usually the most severe consequence in man.
24269369	1	20	from	snake	223:227	arg1	Europe					232:237	Europe	232:237	Europe	232:237	In the envenomation caused by a bite of Vipera ammodytes ammodytes, the most venomous snake in Europe, hemorrhage is usually the most severe consequence in man.
24269369	12	21	theme	same	1680:1683	arg1	venom					1685:1689	the same venom	1676:1689	the same venom	1676:1689	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	7	22	theme	three-dimensional	965:981	arg1	model					983:987	A three-dimensional model	963:987	A three-dimensional model of the VaH4 dimer	963:1005	A three-dimensional model of the VaH4 dimer suggests that Cys132 serves this function.
24269369	13	23	from	proteins	1924:1931	arg1	particular					1937:1946	particular	1937:1946	particular	1937:1946	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	8	24	from	dimers	1068:1073	arg1	class					1088:1092	the P-III class	1078:1092	the P-III class of SVMPs	1078:1101	This implies that dimers in the P-III class of SVMPs can be formed either between their Cys132 or Cys174 residues.
24269369	1	25	theme	severe	271:276	arg1	consequence					278:288	the most severe consequence	262:288	the most severe consequence in man	262:295	In the envenomation caused by a bite of Vipera ammodytes ammodytes, the most venomous snake in Europe, hemorrhage is usually the most severe consequence in man.
24269369	1	25	theme	severe	271:276	arg1	snake					223:227	the most venomous snake	205:227	the most venomous snake in Europe, hemorrhage	205:249	In the envenomation caused by a bite of Vipera ammodytes ammodytes, the most venomous snake in Europe, hemorrhage is usually the most severe consequence in man.
24269369	10	26	theme	VaH4	1412:1415	arg1	kDa					1470:1472	110.2 kDa	1464:1472	110.2 kDa	1464:1472	The molecular mass of VaH4, determined by MALDI/TOF mass spectrometry, is 110.2 kDa.
24269369	10	26	theme	VaH4	1412:1415	arg1	mass					1404:1407	The molecular mass	1390:1407	The molecular mass	1390:1407	The molecular mass of VaH4, determined by MALDI/TOF mass spectrometry, is 110.2 kDa.
24269369	9	27	dep	activity	1181:1188	arg1	The					1165:1167	The	1165:1167	The	1165:1167	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	13	28	theme	ECM	1851:1853	arg1	components					1833:1842	components	1833:1842	components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen	1833:1972	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	5	29	theme	snake	721:725	arg1	domain					839:844	a cysteine-rich domain	823:844	a cysteine-rich domain	823:844	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	5	29	theme	snake	721:725	arg1	SVMPs					753:757	SVMPs	753:757	SVMPs	753:757	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	5	29	theme	snake	721:725	arg1	metalloproteinases					733:750	snake venom metalloproteinases	721:750	snake venom metalloproteinases (SVMPs)	721:758	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	5	29	theme	snake	721:725	arg1	domain					812:817	a disintegrin-like domain	793:817	a disintegrin-like domain	793:817	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	5	30	theme	Complete	597:604	arg1	characterization					617:632	Complete structural characterization	597:632	Complete structural characterization of A and partial characterization of B	597:671	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	12	31	theme	different	1613:1621	arg1	positions					1623:1631	completely different positions	1602:1631	completely different positions from those in homodimeric P-IIIc VaH3 from the same venom	1602:1689	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	10	32	theme	MALDI/TOF	1432:1440	arg1	spectrometry					1447:1458	MALDI/TOF mass spectrometry	1432:1458	MALDI/TOF mass spectrometry	1432:1458	The molecular mass of VaH4, determined by MALDI/TOF mass spectrometry, is 110.2 kDa.
24269369	3	33	theme	high	469:472	arg1	hemorrhagin					489:499	a novel high molecular mass hemorrhagin	461:499	a novel high molecular mass hemorrhagin	461:499	We describe a novel high molecular mass hemorrhagin, VaH4.
24269369	3	33	theme	high	469:472	arg1	VaH4					502:505	VaH4	502:505	VaH4	502:505	We describe a novel high molecular mass hemorrhagin, VaH4.
24269369	0	34	theme	potential	106:114	arg1	activity					127:134	a potential antitumour activity	104:134	a potential antitumour activity	104:134	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	5	35	theme	disintegrin-like	795:810	arg1	metalloproteinases					733:750	snake venom metalloproteinases	721:750	snake venom metalloproteinases (SVMPs)	721:758	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	5	35	theme	disintegrin-like	795:810	arg1	domain					812:817	a disintegrin-like domain	793:817	a disintegrin-like domain	793:817	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	13	36	dep	particular	1937:1946	arg1	α-chain					1952:1958	the α-chain	1948:1958	particular the α-chain of fibrinogen	1937:1972	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	2	37	theme	patients	439:446	arg1	treatment					426:434	medical treatment	418:434	medical treatment of patients	418:446	Identifying and understanding the hemorrhagic components of its venom is therefore particularly important in optimizing medical treatment of patients.
24269369	13	38	theme	proteins	1924:1931	arg1	components					1833:1842	components	1833:1842	components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen	1833:1972	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	13	39	theme	SVMP	1795:1798	arg1	activity					1762:1769	The hemorrhagic activity	1746:1769	The hemorrhagic activity of this slightly acidic SVMP	1746:1798	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	3	40	theme	novel	463:467	arg1	hemorrhagin					489:499	a novel high molecular mass hemorrhagin	461:499	a novel high molecular mass hemorrhagin	461:499	We describe a novel high molecular mass hemorrhagin, VaH4.
24269369	3	40	theme	novel	463:467	arg1	VaH4					502:505	VaH4	502:505	VaH4	502:505	We describe a novel high molecular mass hemorrhagin, VaH4.
24269369	9	41	theme	VaH4	1326:1329	arg1	interaction					1311:1321	physiological interaction	1297:1321	physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM)	1297:1387	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	12	42	from	sites	1571:1575	arg1	VaH4-A					1580:1585	VaH4-A	1580:1585	VaH4-A	1580:1585	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	0	43	theme	covalent	20:27	arg1	VaH4					12:15	Hemorrhagin VaH4	0:15	Hemorrhagin VaH4	0:15	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	0	43	theme	covalent	20:27	arg1	metalloproteinase					49:65	a covalent heterodimeric P-III metalloproteinase	18:65	a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity	18:134	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	12	44	from	venom	1685:1689	arg1	VaH3					1666:1669	homodimeric P-IIIc VaH3	1647:1669	homodimeric P-IIIc VaH3 from the same venom	1647:1689	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	2	45	theme	hemorrhagic	332:342	arg1	components					344:353	the hemorrhagic components	328:353	the hemorrhagic components of its venom	328:366	Identifying and understanding the hemorrhagic components of its venom is therefore particularly important in optimizing medical treatment of patients.
24269369	0	46	theme	P-III	43:47	arg1	VaH4					12:15	Hemorrhagin VaH4	0:15	Hemorrhagin VaH4	0:15	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	0	46	theme	P-III	43:47	arg1	metalloproteinase					49:65	a covalent heterodimeric P-III metalloproteinase	18:65	a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity	18:134	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	3	47	theme	molecular	474:482	arg1	hemorrhagin					489:499	a novel high molecular mass hemorrhagin	461:499	a novel high molecular mass hemorrhagin	461:499	We describe a novel high molecular mass hemorrhagin, VaH4.
24269369	3	47	theme	molecular	474:482	arg1	VaH4					502:505	VaH4	502:505	VaH4	502:505	We describe a novel high molecular mass hemorrhagin, VaH4.
24269369	1	48	theme	venomous	214:221	arg1	consequence					278:288	the most severe consequence	262:288	the most severe consequence in man	262:295	In the envenomation caused by a bite of Vipera ammodytes ammodytes, the most venomous snake in Europe, hemorrhage is usually the most severe consequence in man.
24269369	1	48	theme	venomous	214:221	arg1	snake					223:227	the most venomous snake	205:227	the most venomous snake in Europe, hemorrhage	205:249	In the envenomation caused by a bite of Vipera ammodytes ammodytes, the most venomous snake in Europe, hemorrhage is usually the most severe consequence in man.
24269369	8	49	theme	SVMPs	1097:1101	arg1	class					1088:1092	the P-III class	1078:1092	the P-III class of SVMPs	1078:1101	This implies that dimers in the P-III class of SVMPs can be formed either between their Cys132 or Cys174 residues.
24269369	14	50	theme	potential	2189:2197	arg1	proteins					2165:2172	physiologically very important human proteins	2128:2172	physiologically very important human proteins of therapeutic potential	2128:2197	VaH4 is also significant medically as we found it cytotoxic against cancer cells and due to its substantial sequence similarity to ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential.
24269369	2	51	theme	venom	362:366	arg1	components					344:353	the hemorrhagic components	328:353	the hemorrhagic components of its venom	328:366	Identifying and understanding the hemorrhagic components of its venom is therefore particularly important in optimizing medical treatment of patients.
24269369	9	52	theme	matrix	1376:1381	arg1	element					1347:1353	the latter element	1336:1353	the latter element of the extracellular matrix (ECM)	1336:1387	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	5	53	theme	partial	643:649	arg1	characterization					651:666	partial characterization	643:666	partial characterization	643:666	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	4	54	theme	subunits	568:575	arg1	molecule					521:528	The isolated molecule	508:528	The isolated molecule	508:528	The isolated molecule is a covalent dimer of two homologous subunits, VaH4-A and VaH4-B.
24269369	4	54	theme	subunits	568:575	arg1	dimer					544:548	a covalent dimer	533:548	a covalent dimer of two homologous subunits, VaH4-A and VaH4-B	533:594	The isolated molecule is a covalent dimer of two homologous subunits, VaH4-A and VaH4-B.
24269369	13	55	theme	blood	1906:1910	arg1	proteins					1924:1931	some blood coagulation proteins	1901:1931	some blood coagulation proteins	1901:1931	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	12	56	theme	functional	1721:1730	arg1	implications					1732:1743	any evident functional implications	1709:1743	any evident functional implications	1709:1743	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	12	57	from	positions	1623:1631	arg1	located					1591:1597	located	1591:1597	located	1591:1597	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	12	57	from	positions	1623:1631	arg1	sites					1571:1575	The two possible N-glycosylation sites	1538:1575	The two possible N-glycosylation sites in VaH4-A	1538:1585	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	9	58	theme	proteolytic	1169:1179	arg1	activity					1181:1188	proteolytic activity	1169:1188	proteolytic activity	1169:1188	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	13	59	from	particular	1937:1946	arg1	components					1833:1842	components	1833:1842	components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen	1833:1972	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	5	60	theme	A	637:637	arg1	characterization					617:632	Complete structural characterization	597:632	Complete structural characterization of A and partial characterization of B	597:671	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	1	61	theme	Vipera	177:182	arg1	bite					169:172	a bite	167:172	a bite of Vipera	167:182	In the envenomation caused by a bite of Vipera ammodytes ammodytes, the most venomous snake in Europe, hemorrhage is usually the most severe consequence in man.
24269369	12	62	theme	N-glycosylation	1555:1569	arg1	located					1591:1597	located	1591:1597	located	1591:1597	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	12	62	theme	N-glycosylation	1555:1569	arg1	sites					1571:1575	The two possible N-glycosylation sites	1538:1575	The two possible N-glycosylation sites in VaH4-A	1538:1585	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	14	63	theme	ADAM/ADAMTS	2106:2116	arg1	family					2118:2123	ADAM/ADAMTS family	2106:2123	ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential	2106:2197	VaH4 is also significant medically as we found it cytotoxic against cancer cells and due to its substantial sequence similarity to ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential.
24269369	11	64	theme	monomer	1518:1524	arg1	mass					1505:1508	the mass	1501:1508	the mass of each monomer by 8.7 kDa	1501:1535	N-deglycosylation reduced the mass of each monomer by 8.7 kDa.
24269369	6	65	theme	SVMPs	956:960	arg1	bond					942:945	the inter-subunit disulphide bond	913:945	the inter-subunit disulphide bond of P-III SVMPs	913:960	However, neither VaH4-A nor VaH4-B possess the Cys174 involved in the inter-subunit disulphide bond of P-III SVMPs.
24269369	5	66	theme	P-III	706:710	arg1	class					712:716	the P-III class	702:716	the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain	702:844	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	12	67	theme	P-IIIc	1659:1664	arg1	VaH3					1666:1669	homodimeric P-IIIc VaH3	1647:1669	homodimeric P-IIIc VaH3 from the same venom	1647:1689	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	0	68	from	Vipera	72:77	arg1	VaH4					12:15	Hemorrhagin VaH4	0:15	Hemorrhagin VaH4	0:15	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	0	68	from	Vipera	72:77	arg1	metalloproteinase					49:65	a covalent heterodimeric P-III metalloproteinase	18:65	a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity	18:134	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	14	69	theme	substantial	2071:2081	arg1	similarity					2092:2101	its substantial sequence similarity	2067:2101	its substantial sequence similarity to ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential	2067:2197	VaH4 is also significant medically as we found it cytotoxic against cancer cells and due to its substantial sequence similarity to ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential.
24269369	9	70	theme	Zn²⁺	1222:1225	arg1	ions					1236:1239	Zn²⁺ and Ca²⁺ ions	1222:1239	Zn²⁺ and Ca²⁺ ions	1222:1239	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	6	71	theme	disulphide	931:940	arg1	bond					942:945	the inter-subunit disulphide bond	913:945	the inter-subunit disulphide bond of P-III SVMPs	913:960	However, neither VaH4-A nor VaH4-B possess the Cys174 involved in the inter-subunit disulphide bond of P-III SVMPs.
24269369	9	72	theme	Ca²⁺	1231:1234	arg1	ions					1236:1239	Zn²⁺ and Ca²⁺ ions	1222:1239	Zn²⁺ and Ca²⁺ ions	1222:1239	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	7	73	theme	dimer	1001:1005	arg1	model					983:987	A three-dimensional model	963:987	A three-dimensional model of the VaH4 dimer	963:1005	A three-dimensional model of the VaH4 dimer suggests that Cys132 serves this function.
24269369	12	74	from	those	1638:1642	arg1	positions					1623:1631	completely different positions	1602:1631	completely different positions from those in homodimeric P-IIIc VaH3 from the same venom	1602:1689	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	13	75	theme	components	1833:1842	arg1	hydrolysis					1819:1828	its hydrolysis	1815:1828	its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen	1815:1972	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	14	76	dep	important	2149:2157	arg1	human					2159:2163	human	2159:2163	human	2159:2163	VaH4 is also significant medically as we found it cytotoxic against cancer cells and due to its substantial sequence similarity to ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential.
24269369	4	77	theme	isolated	512:519	arg1	molecule					521:528	The isolated molecule	508:528	The isolated molecule	508:528	The isolated molecule is a covalent dimer of two homologous subunits, VaH4-A and VaH4-B.
24269369	4	77	theme	isolated	512:519	arg1	dimer					544:548	a covalent dimer	533:548	a covalent dimer of two homologous subunits, VaH4-A and VaH4-B	533:594	The isolated molecule is a covalent dimer of two homologous subunits, VaH4-A and VaH4-B.
24269369	9	78	theme	glycosaminoglycans	1261:1278	arg1	ions					1236:1239	Zn²⁺ and Ca²⁺ ions	1222:1239	Zn²⁺ and Ca²⁺ ions	1222:1239	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	9	78	theme	glycosaminoglycans	1261:1278	arg1	presence					1249:1256	the presence	1245:1256	the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM)	1245:1387	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	12	79	theme	homodimeric	1647:1657	arg1	VaH3					1666:1669	homodimeric P-IIIc VaH3	1647:1669	homodimeric P-IIIc VaH3 from the same venom	1647:1689	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	10	80	theme	molecular	1394:1402	arg1	kDa					1470:1472	110.2 kDa	1464:1472	110.2 kDa	1464:1472	The molecular mass of VaH4, determined by MALDI/TOF mass spectrometry, is 110.2 kDa.
24269369	10	80	theme	molecular	1394:1402	arg1	mass					1404:1407	The molecular mass	1390:1407	The molecular mass	1390:1407	The molecular mass of VaH4, determined by MALDI/TOF mass spectrometry, is 110.2 kDa.
24269369	0	81	theme	antitumour	116:125	arg1	activity					127:134	a potential antitumour activity	104:134	a potential antitumour activity	104:134	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	5	82	theme	structural	606:615	arg1	characterization					617:632	Complete structural characterization	597:632	Complete structural characterization of A and partial characterization of B	597:671	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	13	83	from	components	1833:1842	arg1	particular					1937:1946	particular	1937:1946	particular	1937:1946	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	12	84	located	located	1591:1597	arg2	sites					1571:1575	The two possible N-glycosylation sites	1538:1575	The two possible N-glycosylation sites in VaH4-A	1538:1585	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	12	84	located	located	1591:1597	arg1	positions					1623:1631	completely different positions	1602:1631	completely different positions from those in homodimeric P-IIIc VaH3 from the same venom	1602:1689	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	12	84	located	located	1591:1597	arg2	located					1591:1597	located	1591:1597	located	1591:1597	The two possible N-glycosylation sites in VaH4-A are located at completely different positions from those in homodimeric P-IIIc VaH3 from the same venom, however, without any evident functional implications.
24269369	5	85	theme	venom	727:731	arg1	domain					839:844	a cysteine-rich domain	823:844	a cysteine-rich domain	823:844	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	5	85	theme	venom	727:731	arg1	SVMPs					753:757	SVMPs	753:757	SVMPs	753:757	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	5	85	theme	venom	727:731	arg1	metalloproteinases					733:750	snake venom metalloproteinases	721:750	snake venom metalloproteinases (SVMPs)	721:758	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	5	85	theme	venom	727:731	arg1	domain					812:817	a disintegrin-like domain	793:817	a disintegrin-like domain	793:817	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	9	86	theme	physiological	1297:1309	arg1	interaction					1311:1321	physiological interaction	1297:1321	physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM)	1297:1387	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	14	87	theme	cancer	2043:2048	arg1	cells					2050:2054	cancer cells	2043:2054	cancer cells	2043:2054	VaH4 is also significant medically as we found it cytotoxic against cancer cells and due to its substantial sequence similarity to ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential.
24269369	13	88	theme	acidic	1788:1793	arg1	SVMP					1795:1798	this slightly acidic SVMP	1774:1798	this slightly acidic SVMP	1774:1798	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	4	89	theme	homologous	557:566	arg1	VaH4-A					578:583	VaH4-A	578:583	VaH4-A	578:583	The isolated molecule is a covalent dimer of two homologous subunits, VaH4-A and VaH4-B.
24269369	4	89	theme	homologous	557:566	arg1	subunits					568:575	two homologous subunits	553:575	two homologous subunits	553:575	The isolated molecule is a covalent dimer of two homologous subunits, VaH4-A and VaH4-B.
24269369	4	89	theme	homologous	557:566	arg1	VaH4-B					589:594	VaH4-B	589:594	VaH4-B	589:594	The isolated molecule is a covalent dimer of two homologous subunits, VaH4-A and VaH4-B.
24269369	2	90	theme	medical	418:424	arg1	treatment					426:434	medical treatment	418:434	medical treatment of patients	418:446	Identifying and understanding the hemorrhagic components of its venom is therefore particularly important in optimizing medical treatment of patients.
24269369	0	91	theme	heterodimeric	29:41	arg1	VaH4					12:15	Hemorrhagin VaH4	0:15	Hemorrhagin VaH4	0:15	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	0	91	theme	heterodimeric	29:41	arg1	metalloproteinase					49:65	a covalent heterodimeric P-III metalloproteinase	18:65	a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity	18:134	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	8	92	theme	P-III	1082:1086	arg1	class					1088:1092	the P-III class	1078:1092	the P-III class of SVMPs	1078:1101	This implies that dimers in the P-III class of SVMPs can be formed either between their Cys132 or Cys174 residues.
24269369	10	93	theme	mass	1442:1445	arg1	spectrometry					1447:1458	MALDI/TOF mass spectrometry	1432:1458	MALDI/TOF mass spectrometry	1432:1458	The molecular mass of VaH4, determined by MALDI/TOF mass spectrometry, is 110.2 kDa.
24269369	5	94	theme	metalloproteinases	733:750	arg1	class					712:716	the P-III class	702:716	the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain	702:844	Complete structural characterization of A and partial characterization of B revealed that both belong to the P-III class of snake venom metalloproteinases (SVMPs), comprising a metalloproteinase, a disintegrin-like domain and a cysteine-rich domain.
24269369	9	95	theme	latter	1340:1345	arg1	element					1347:1353	the latter element	1336:1353	the latter element of the extracellular matrix (ECM)	1336:1387	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	4	96	theme	covalent	535:542	arg1	molecule					521:528	The isolated molecule	508:528	The isolated molecule	508:528	The isolated molecule is a covalent dimer of two homologous subunits, VaH4-A and VaH4-B.
24269369	4	96	theme	covalent	535:542	arg1	dimer					544:548	a covalent dimer	533:548	a covalent dimer of two homologous subunits, VaH4-A and VaH4-B	533:594	The isolated molecule is a covalent dimer of two homologous subunits, VaH4-A and VaH4-B.
24269369	13	97	theme	fibrinogen	1963:1972	arg1	α-chain					1952:1958	the α-chain	1948:1958	particular the α-chain of fibrinogen	1937:1972	The hemorrhagic activity of this slightly acidic SVMP is ascribed to its hydrolysis of components of the ECM, particularly fibronectin and nidogen, and of some blood coagulation proteins, in particular the α-chain of fibrinogen.
24269369	0	98	with	metalloproteinase	49:65	arg1	activity					127:134	a potential antitumour activity	104:134	a potential antitumour activity	104:134	Hemorrhagin VaH4, a covalent heterodimeric P-III metalloproteinase from Vipera ammodytes ammodytes with a potential antitumour activity.
24269369	3	99	theme	mass	484:487	arg1	hemorrhagin					489:499	a novel high molecular mass hemorrhagin	461:499	a novel high molecular mass hemorrhagin	461:499	We describe a novel high molecular mass hemorrhagin, VaH4.
24269369	3	99	theme	mass	484:487	arg1	VaH4					502:505	VaH4	502:505	VaH4	502:505	We describe a novel high molecular mass hemorrhagin, VaH4.
24269369	9	100	theme	extracellular	1362:1374	arg1	matrix					1376:1381	the extracellular matrix	1358:1381	the extracellular matrix (ECM)	1358:1387	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	9	100	theme	extracellular	1362:1374	arg1	ECM					1384:1386	ECM	1384:1386	ECM	1384:1386	The proteolytic activity and stability of VaH4 depend on Zn²⁺ and Ca²⁺ ions and the presence of glycosaminoglycans, which indicates physiological interaction of VaH4 with the latter element of the extracellular matrix (ECM).
24269369	14	101	theme	therapeutic	2177:2187	arg1	potential					2189:2197	therapeutic potential	2177:2197	therapeutic potential	2177:2197	VaH4 is also significant medically as we found it cytotoxic against cancer cells and due to its substantial sequence similarity to ADAM/ADAMTS family of physiologically very important human proteins of therapeutic potential.
25190208	3	0	theme	Freeze-dry	354:363	arg1	criterion					388:396	criterion	388:396	criterion for excipients and formulation selection	388:437	Freeze-dry microscopy was used as criterion for excipients and formulation selection; as well as to define freeze-drying parameters.
25190208	3	0	theme	Freeze-dry	354:363	arg1	microscopy					365:374	Freeze-dry microscopy	354:374	Freeze-dry microscopy	354:374	Freeze-dry microscopy was used as criterion for excipients and formulation selection; as well as to define freeze-drying parameters.
25190208	4	1	theme	freeze-drying	547:559	arg1	recovery					561:568	freeze-drying recovery	547:568	freeze-drying recovery	547:568	Excipients screening were evaluated through their effect on freeze-drying recovery and dried product stability at 50 °C by using a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity.
25190208	5	2	theme	trehalose	828:836	arg1	addition					805:812	the addition	801:812	the addition of sucrose or trehalose	801:836	The highest stability of rhEGF during freeze-drying was achieved by the addition of sucrose or trehalose.
25190208	1	3	from	stabilization	197:209	arg1	form					269:272	a lyophilized dosage form	248:272	a lyophilized dosage form	248:272	Development studies were performed to design a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form.
25190208	7	4	theme	delivery	1096:1103	arg1	processes					1105:1113	the freeze-drying and delivery processes	1074:1113	the freeze-drying and delivery processes	1074:1113	The selected formulation mixture of sucrose and dextran could prevent protein degradation during the freeze-drying and delivery processes.
25190208	6	5	theme	trehalose	953:961	arg1	addition					923:930	the addition	919:930	the addition of dextran, sucrose, trehalose or raffinose	919:974	After storing the dried product at 50 °C, the highest stability was achieved by the addition of dextran, sucrose, trehalose or raffinose.
25190208	9	6	theme	technical	1342:1350	arg1	solution					1352:1359	an appropriate technical solution	1327:1359	an appropriate technical solution for stabilizing rhEGF	1327:1381	These results indicate that the freeze-dried formulation represents an appropriate technical solution for stabilizing rhEGF.
25190208	4	7	theme	comprehensive	618:630	arg1	set					632:634	a comprehensive set	616:634	a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity	616:730	Excipients screening were evaluated through their effect on freeze-drying recovery and dried product stability at 50 °C by using a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity.
25190208	4	7	theme	comprehensive	618:630	arg1	techniques					650:659	analytical techniques	639:659	analytical techniques assessing the chemical stability, protein conformation and bioactivity	639:730	Excipients screening were evaluated through their effect on freeze-drying recovery and dried product stability at 50 °C by using a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity.
25190208	6	8	theme	sucrose	944:950	arg1	addition					923:930	the addition	919:930	the addition of dextran, sucrose, trehalose or raffinose	919:974	After storing the dried product at 50 °C, the highest stability was achieved by the addition of dextran, sucrose, trehalose or raffinose.
25190208	3	9	used	used	380:383	arg2	criterion					388:396	criterion	388:396	criterion for excipients and formulation selection	388:437	Freeze-dry microscopy was used as criterion for excipients and formulation selection; as well as to define freeze-drying parameters.
25190208	3	9	used	used	380:383	arg2	microscopy					365:374	Freeze-dry microscopy	354:374	Freeze-dry microscopy	354:374	Freeze-dry microscopy was used as criterion for excipients and formulation selection; as well as to define freeze-drying parameters.
25190208	7	10	theme	sucrose	1013:1019	arg1	mixture					1002:1008	The selected formulation mixture	977:1008	The selected formulation mixture of sucrose and dextran	977:1031	The selected formulation mixture of sucrose and dextran could prevent protein degradation during the freeze-drying and delivery processes.
25190208	7	11	theme	dextran	1025:1031	arg1	mixture					1002:1008	The selected formulation mixture	977:1008	The selected formulation mixture of sucrose and dextran	977:1031	The selected formulation mixture of sucrose and dextran could prevent protein degradation during the freeze-drying and delivery processes.
25190208	4	12	theme	analytical	639:648	arg1	techniques					650:659	analytical techniques	639:659	analytical techniques assessing the chemical stability, protein conformation and bioactivity	639:730	Excipients screening were evaluated through their effect on freeze-drying recovery and dried product stability at 50 °C by using a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity.
25190208	8	13	theme	degradation	1120:1130	arg1	rate					1132:1135	The degradation rate	1116:1135	The degradation rate assessed by RP-HPLC	1116:1155	The degradation rate assessed by RP-HPLC could decrease 100 times at 37 °C and 70 times at 50 °C in dried with respect to aqueous formulation.
25190208	7	14	theme	formulation	990:1000	arg1	mixture					1002:1008	The selected formulation mixture	977:1008	The selected formulation mixture of sucrose and dextran	977:1031	The selected formulation mixture of sucrose and dextran could prevent protein degradation during the freeze-drying and delivery processes.
25190208	6	15	theme	dextran	935:941	arg1	addition					923:930	the addition	919:930	the addition of dextran, sucrose, trehalose or raffinose	919:974	After storing the dried product at 50 °C, the highest stability was achieved by the addition of dextran, sucrose, trehalose or raffinose.
25190208	4	16	from	effect	537:542	arg1	recovery					561:568	freeze-drying recovery	547:568	freeze-drying recovery	547:568	Excipients screening were evaluated through their effect on freeze-drying recovery and dried product stability at 50 °C by using a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity.
25190208	6	17	theme	dried	857:861	arg1	product					863:869	the dried product	853:869	the dried product	853:869	After storing the dried product at 50 °C, the highest stability was achieved by the addition of dextran, sucrose, trehalose or raffinose.
25190208	2	18	theme	drying	299:304	arg1	protocols					306:314	Unannealed and annealed drying protocols	275:314	Unannealed and annealed drying protocols	275:314	Unannealed and annealed drying protocols were tested for excipients screening.
25190208	5	19	theme	sucrose	817:823	arg1	addition					805:812	the addition	801:812	the addition of sucrose or trehalose	801:836	The highest stability of rhEGF during freeze-drying was achieved by the addition of sucrose or trehalose.
25190208	5	20	theme	rhEGF	758:762	arg1	stability					745:753	The highest stability	733:753	The highest stability of rhEGF during freeze-drying	733:783	The highest stability of rhEGF during freeze-drying was achieved by the addition of sucrose or trehalose.
25190208	1	21	theme	Development	107:117	arg1	studies					119:125	Development studies	107:125	Development studies	107:125	Development studies were performed to design a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form.
25190208	3	22	theme	freeze-drying	461:473	arg1	parameters					475:484	freeze-drying parameters	461:484	freeze-drying parameters	461:484	Freeze-dry microscopy was used as criterion for excipients and formulation selection; as well as to define freeze-drying parameters.
25190208	0	23	theme	recombinant	19:29	arg1	factor					54:59	a recombinant human epidermal growth factor	17:59	a recombinant human epidermal growth factor parenteral formulation	17:82	Stabilization of a recombinant human epidermal growth factor parenteral formulation through freeze-drying.
25190208	2	24	theme	excipients	332:341	arg1	screening					343:351	excipients screening	332:351	excipients screening	332:351	Unannealed and annealed drying protocols were tested for excipients screening.
25190208	1	25	theme	parenteral	216:225	arg1	formulation					233:243	a parenteral rhEGF formulation	214:243	a parenteral rhEGF formulation in a lyophilized dosage form	214:272	Development studies were performed to design a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form.
25190208	1	26	theme	rhEGF	227:231	arg1	formulation					233:243	a parenteral rhEGF formulation	214:243	a parenteral rhEGF formulation in a lyophilized dosage form	214:272	Development studies were performed to design a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form.
25190208	0	27	theme	epidermal	37:45	arg1	factor					54:59	a recombinant human epidermal growth factor	17:59	a recombinant human epidermal growth factor parenteral formulation	17:82	Stabilization of a recombinant human epidermal growth factor parenteral formulation through freeze-drying.
25190208	4	28	theme	Excipients	487:496	arg1	screening					498:506	Excipients screening	487:506	Excipients screening	487:506	Excipients screening were evaluated through their effect on freeze-drying recovery and dried product stability at 50 °C by using a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity.
25190208	9	29	theme	appropriate	1330:1340	arg1	solution					1352:1359	an appropriate technical solution	1327:1359	an appropriate technical solution for stabilizing rhEGF	1327:1381	These results indicate that the freeze-dried formulation represents an appropriate technical solution for stabilizing rhEGF.
25190208	1	30	theme	formulation	233:243	arg1	stabilization					197:209	the stabilization	193:209	the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form	193:272	Development studies were performed to design a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form.
25190208	0	31	theme	human	31:35	arg1	factor					54:59	a recombinant human epidermal growth factor	17:59	a recombinant human epidermal growth factor parenteral formulation	17:82	Stabilization of a recombinant human epidermal growth factor parenteral formulation through freeze-drying.
25190208	8	32	from	°C	1188:1189	arg1	times					1176:1180	100 times	1172:1180	100 times at 37 °C	1172:1189	The degradation rate assessed by RP-HPLC could decrease 100 times at 37 °C and 70 times at 50 °C in dried with respect to aqueous formulation.
25190208	8	32	from	°C	1188:1189	arg1	times					1198:1202	70 times	1195:1202	70 times at 50 °C	1195:1211	The degradation rate assessed by RP-HPLC could decrease 100 times at 37 °C and 70 times at 50 °C in dried with respect to aqueous formulation.
25190208	4	33	theme	chemical	675:682	arg1	stability					684:692	the chemical stability	671:692	the chemical stability	671:692	Excipients screening were evaluated through their effect on freeze-drying recovery and dried product stability at 50 °C by using a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity.
25190208	1	34	from	formulation	233:243	arg1	form					269:272	a lyophilized dosage form	248:272	a lyophilized dosage form	248:272	Development studies were performed to design a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form.
25190208	4	35	theme	protein	695:701	arg1	conformation					703:714	protein conformation	695:714	protein conformation	695:714	Excipients screening were evaluated through their effect on freeze-drying recovery and dried product stability at 50 °C by using a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity.
25190208	0	36	theme	factor	54:59	arg1	formulation					72:82	a recombinant human epidermal growth factor parenteral formulation	17:82	a recombinant human epidermal growth factor parenteral formulation	17:82	Stabilization of a recombinant human epidermal growth factor parenteral formulation through freeze-drying.
25190208	4	37	theme	product	580:586	arg1	stability					588:596	product stability	580:596	product stability	580:596	Excipients screening were evaluated through their effect on freeze-drying recovery and dried product stability at 50 °C by using a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity.
25190208	5	38	theme	highest	737:743	arg1	stability					745:753	The highest stability	733:753	The highest stability of rhEGF during freeze-drying	733:783	The highest stability of rhEGF during freeze-drying was achieved by the addition of sucrose or trehalose.
25190208	0	39	theme	growth	47:52	arg1	factor					54:59	a recombinant human epidermal growth factor	17:59	a recombinant human epidermal growth factor parenteral formulation	17:82	Stabilization of a recombinant human epidermal growth factor parenteral formulation through freeze-drying.
25190208	1	40	from	form	269:272	arg1	stabilization					197:209	the stabilization	193:209	the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form	193:272	Development studies were performed to design a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form.
25190208	8	41	from	°C	1210:1211	arg1	times					1176:1180	100 times	1172:1180	100 times at 37 °C	1172:1189	The degradation rate assessed by RP-HPLC could decrease 100 times at 37 °C and 70 times at 50 °C in dried with respect to aqueous formulation.
25190208	8	41	from	°C	1210:1211	arg1	times					1198:1202	70 times	1195:1202	70 times at 50 °C	1195:1211	The degradation rate assessed by RP-HPLC could decrease 100 times at 37 °C and 70 times at 50 °C in dried with respect to aqueous formulation.
25190208	9	42	theme	freeze-dried	1291:1302	arg1	formulation					1304:1314	the freeze-dried formulation	1287:1314	the freeze-dried formulation	1287:1314	These results indicate that the freeze-dried formulation represents an appropriate technical solution for stabilizing rhEGF.
25190208	7	43	theme	protein	1047:1053	arg1	degradation					1055:1065	protein degradation	1047:1065	protein degradation	1047:1065	The selected formulation mixture of sucrose and dextran could prevent protein degradation during the freeze-drying and delivery processes.
25190208	8	44	theme	aqueous	1238:1244	arg1	formulation					1246:1256	aqueous formulation	1238:1256	aqueous formulation	1238:1256	The degradation rate assessed by RP-HPLC could decrease 100 times at 37 °C and 70 times at 50 °C in dried with respect to aqueous formulation.
25190208	6	45	theme	raffinose	966:974	arg1	addition					923:930	the addition	919:930	the addition of dextran, sucrose, trehalose or raffinose	919:974	After storing the dried product at 50 °C, the highest stability was achieved by the addition of dextran, sucrose, trehalose or raffinose.
25190208	7	46	theme	freeze-drying	1078:1090	arg1	processes					1105:1113	the freeze-drying and delivery processes	1074:1113	the freeze-drying and delivery processes	1074:1113	The selected formulation mixture of sucrose and dextran could prevent protein degradation during the freeze-drying and delivery processes.
25190208	6	47	theme	highest	885:891	arg1	stability					893:901	the highest stability	881:901	the highest stability	881:901	After storing the dried product at 50 °C, the highest stability was achieved by the addition of dextran, sucrose, trehalose or raffinose.
25190208	2	48	theme	annealed	290:297	arg1	protocols					306:314	Unannealed and annealed drying protocols	275:314	Unannealed and annealed drying protocols	275:314	Unannealed and annealed drying protocols were tested for excipients screening.
25190208	3	49	dep	excipients	402:411	arg1	selection					429:437	selection	429:437	selection	429:437	Freeze-dry microscopy was used as criterion for excipients and formulation selection; as well as to define freeze-drying parameters.
25190208	7	50	theme	selected	981:988	arg1	mixture					1002:1008	The selected formulation mixture	977:1008	The selected formulation mixture of sucrose and dextran	977:1031	The selected formulation mixture of sucrose and dextran could prevent protein degradation during the freeze-drying and delivery processes.
25190208	1	51	theme	lyophilized	250:260	arg1	form					269:272	a lyophilized dosage form	248:272	a lyophilized dosage form	248:272	Development studies were performed to design a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form.
25190208	0	52	theme	formulation	72:82	arg1	Stabilization					0:12	Stabilization	0:12	Stabilization of a recombinant human epidermal growth factor parenteral formulation through freeze-drying.	0:105	Stabilization of a recombinant human epidermal growth factor parenteral formulation through freeze-drying.
25190208	2	53	theme	Unannealed	275:284	arg1	protocols					306:314	Unannealed and annealed drying protocols	275:314	Unannealed and annealed drying protocols	275:314	Unannealed and annealed drying protocols were tested for excipients screening.
25190208	1	54	theme	pharmaceutical	154:167	arg1	composition					169:179	a pharmaceutical composition	152:179	a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form	152:272	Development studies were performed to design a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form.
25190208	1	55	theme	dosage	262:267	arg1	form					269:272	a lyophilized dosage form	248:272	a lyophilized dosage form	248:272	Development studies were performed to design a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized dosage form.
25190208	0	56	theme	parenteral	61:70	arg1	formulation					72:82	a recombinant human epidermal growth factor parenteral formulation	17:82	a recombinant human epidermal growth factor parenteral formulation	17:82	Stabilization of a recombinant human epidermal growth factor parenteral formulation through freeze-drying.
25190208	4	57	theme	techniques	650:659	arg1	set					632:634	a comprehensive set	616:634	a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity	616:730	Excipients screening were evaluated through their effect on freeze-drying recovery and dried product stability at 50 °C by using a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity.
25190208	4	57	theme	techniques	650:659	arg1	techniques					650:659	analytical techniques	639:659	analytical techniques assessing the chemical stability, protein conformation and bioactivity	639:730	Excipients screening were evaluated through their effect on freeze-drying recovery and dried product stability at 50 °C by using a comprehensive set of analytical techniques assessing the chemical stability, protein conformation and bioactivity.
26505953	4	0	from	effect	663:668	arg1	bioactivity					783:793	bioactivity	783:793	bioactivity	783:793	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	0	from	effect	663:668	arg1	degradation					747:757	degradation	747:757	degradation	747:757	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	0	from	effect	663:668	arg1	adsorption					768:777	protein adsorption	760:777	protein adsorption	760:777	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	0	from	effect	663:668	arg1	swelling					737:744	swelling	737:744	swelling	737:744	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	0	from	effect	663:668	arg1	properties					725:734	the mechanical and in vitro biological properties	686:734	the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds	686:821	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	7	1	contain	has	1285:1287	arg2	potential					1294:1302	wide potential	1289:1302	wide potential	1289:1302	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	7	1	contain	has	1285:1287	arg1	scaffold					1216:1223	the novel CS-OM-HA composite scaffold	1187:1223	the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties	1187:1283	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	3	2	theme	mercury	563:569	arg1	MIP					594:596	MIP	594:596	MIP	594:596	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	3	2	theme	mercury	563:569	arg1	porosimeter					581:591	mercury intrusion porosimeter	563:591	mercury intrusion porosimeter (MIP)	563:597	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	6	3	theme	composite	1058:1066	arg1	scaffolds					1068:1076	CS-OM-HA composite scaffolds	1049:1076	CS-OM-HA composite scaffolds	1049:1076	CS-OM-HA composite scaffolds were also found to be non-cytotoxic to MG 63 osteoblast cell lines.
26505953	5	4	theme	pure	1010:1013	arg1	scaffolds					1038:1046	the pure CS and CS-OM composite scaffolds	1006:1046	the pure CS and CS-OM composite scaffolds	1006:1046	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	5	5	theme	scaffolds	948:956	arg1	studies					918:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies	839:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds	839:956	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	3	6	theme	intrusion	571:579	arg1	MIP					594:596	MIP	594:596	MIP	594:596	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	3	6	theme	intrusion	571:579	arg1	porosimeter					581:591	mercury intrusion porosimeter	563:591	mercury intrusion porosimeter (MIP)	563:597	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	5	7	theme	composite	1028:1036	arg1	scaffolds					1038:1046	the pure CS and CS-OM composite scaffolds	1006:1046	the pure CS and CS-OM composite scaffolds	1006:1046	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	7	8	theme	mechanical	1239:1248	arg1	properties					1274:1283	improved mechanical and in vitro biological properties	1230:1283	improved mechanical and in vitro biological properties	1230:1283	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	2	9	theme	nm	340:341	arg1	particles					317:325	Hydroxyapatite (HA) particles	297:325	Hydroxyapatite (HA) particles of size ∼ 65 nm	297:341	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	3	10	theme	microscopy	621:630	arg1	studies					638:644	scanning electron microscopy (SEM) studies	603:644	scanning electron microscopy (SEM) studies	603:644	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	7	11	theme	improved	1230:1237	arg1	properties					1274:1283	improved mechanical and in vitro biological properties	1230:1283	improved mechanical and in vitro biological properties	1230:1283	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	7	12	theme	wide	1289:1292	arg1	potential					1294:1302	wide potential	1289:1302	wide potential	1289:1302	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	3	13	theme	scanning	603:610	arg1	microscopy					621:630	scanning electron microscopy	603:630	scanning electron microscopy (SEM) studies	603:644	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	3	13	theme	scanning	603:610	arg1	SEM					633:635	SEM	633:635	SEM	633:635	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	5	14	theme	composite	938:946	arg1	scaffolds					948:956	CS-OM-HA composite scaffolds	929:956	CS-OM-HA composite scaffolds	929:956	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	5	15	theme	bioactivity	883:893	arg1	studies					918:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies	839:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds	839:956	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	7	16	theme	bone	1324:1327	arg1	applications					1348:1359	bone tissue engineering applications	1324:1359	bone tissue engineering applications	1324:1359	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	7	17	theme	CS-OM-HA	1197:1204	arg1	scaffold					1216:1223	the novel CS-OM-HA composite scaffold	1187:1223	the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties	1187:1283	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	6	18	theme	cell	1134:1137	arg1	lines					1139:1143	MG 63 osteoblast cell lines	1117:1143	MG 63 osteoblast cell lines	1117:1143	CS-OM-HA composite scaffolds were also found to be non-cytotoxic to MG 63 osteoblast cell lines.
26505953	2	19	theme	∼	335:335	arg1	nm					340:341	size ∼ 65 nm	330:341	size ∼ 65 nm	330:341	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	2	20	theme	infrared	456:463	arg1	spectroscopy					476:487	infrared (ATR-FTIR) spectroscopy	456:487	attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy	409:487	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	5	21	theme	Swelling	839:846	arg1	studies					918:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies	839:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds	839:956	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	2	22	theme	size	330:333	arg1	nm					340:341	size ∼ 65 nm	330:341	size ∼ 65 nm	330:341	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	3	23	theme	composite	503:511	arg1	scaffolds					513:521	The prepared composite scaffolds	490:521	The prepared composite scaffolds	490:521	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	7	24	theme	composite	1206:1214	arg1	scaffold					1216:1223	the novel CS-OM-HA composite scaffold	1187:1223	the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties	1187:1283	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	7	25	theme	engineering	1336:1346	arg1	applications					1348:1359	bone tissue engineering applications	1324:1359	bone tissue engineering applications	1324:1359	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	2	26	theme	reflectance-Fourier	426:444	arg1	spectroscopy					476:487	infrared (ATR-FTIR) spectroscopy	456:487	attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy	409:487	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	0	27	theme	Microwave-assisted	0:17	arg1	synthesis					19:27	Microwave-assisted synthesis	0:27	Microwave-assisted synthesis of porous	0:37	Microwave-assisted synthesis of porous chitosan-modified montmorillonite-hydroxyapatite composite scaffolds.
26505953	4	28	dep	properties	725:734	arg1	bioactivity					783:793	bioactivity	783:793	bioactivity	783:793	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	28	dep	properties	725:734	arg1	degradation					747:757	degradation	747:757	degradation	747:757	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	28	dep	properties	725:734	arg1	adsorption					768:777	protein adsorption	760:777	protein adsorption	760:777	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	28	dep	properties	725:734	arg1	swelling					737:744	swelling	737:744	swelling	737:744	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	28	dep	properties	725:734	arg1	properties					725:734	the mechanical and in vitro biological properties	686:734	the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds	686:821	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	5	29	theme	protein	899:905	arg1	adsorption					907:916	protein adsorption	899:916	protein adsorption	899:916	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	5	30	theme	adsorption	907:916	arg1	studies					918:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies	839:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds	839:956	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	2	31	theme	total	420:424	arg1	spectroscopy					476:487	infrared (ATR-FTIR) spectroscopy	456:487	attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy	409:487	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	0	32	theme	porous	32:37	arg1	synthesis					19:27	Microwave-assisted synthesis	0:27	Microwave-assisted synthesis of porous	0:37	Microwave-assisted synthesis of porous chitosan-modified montmorillonite-hydroxyapatite composite scaffolds.
26505953	5	33	theme	degradation	849:859	arg1	studies					918:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies	839:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds	839:956	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	7	34	dep	in	1254:1255	arg1	vitro					1257:1261	vitro	1257:1261	vitro	1257:1261	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	1	35	theme	porous	126:131	arg1	scaffold					215:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	In this study, a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold was developed by combining microwave irradiation and gas foaming method.
26505953	6	36	theme	osteoblast	1123:1132	arg1	lines					1139:1143	MG 63 osteoblast cell lines	1117:1143	MG 63 osteoblast cell lines	1117:1143	CS-OM-HA composite scaffolds were also found to be non-cytotoxic to MG 63 osteoblast cell lines.
26505953	4	37	theme	OM	680:681	arg1	effect					663:668	The synergistic effect	647:668	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds	647:821	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	5	38	theme	desirable	969:977	arg1	results					979:985	desirable results	969:985	desirable results	969:985	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	4	39	theme	scaffolds	813:821	arg1	bioactivity					783:793	bioactivity	783:793	bioactivity	783:793	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	39	theme	scaffolds	813:821	arg1	degradation					747:757	degradation	747:757	degradation	747:757	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	39	theme	scaffolds	813:821	arg1	adsorption					768:777	protein adsorption	760:777	protein adsorption	760:777	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	39	theme	scaffolds	813:821	arg1	swelling					737:744	swelling	737:744	swelling	737:744	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	39	theme	scaffolds	813:821	arg1	properties					725:734	the mechanical and in vitro biological properties	686:734	the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds	686:821	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	1	40	theme	chitosan-organically	133:152	arg1	scaffold					215:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	In this study, a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold was developed by combining microwave irradiation and gas foaming method.
26505953	5	41	theme	CS-OM-HA	929:936	arg1	scaffolds					948:956	CS-OM-HA composite scaffolds	929:956	CS-OM-HA composite scaffolds	929:956	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	0	42	theme	montmorillonite-hydroxyapatite	57:86	arg1	scaffolds					98:106	montmorillonite-hydroxyapatite composite scaffolds	57:106	montmorillonite-hydroxyapatite composite scaffolds	57:106	Microwave-assisted synthesis of porous chitosan-modified montmorillonite-hydroxyapatite composite scaffolds.
26505953	5	43	theme	CS	1015:1016	arg1	scaffolds					1038:1046	the pure CS and CS-OM composite scaffolds	1006:1046	the pure CS and CS-OM composite scaffolds	1006:1046	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	2	44	theme	ATR-FTIR	466:473	arg1	spectroscopy					476:487	infrared (ATR-FTIR) spectroscopy	456:487	attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy	409:487	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	1	45	theme	microwave	251:259	arg1	irradiation					261:271	microwave irradiation	251:271	microwave irradiation	251:271	In this study, a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold was developed by combining microwave irradiation and gas foaming method.
26505953	1	46	theme	gas	277:279	arg1	method					289:294	gas foaming method	277:294	gas foaming method	277:294	In this study, a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold was developed by combining microwave irradiation and gas foaming method.
26505953	3	47	theme	prepared	494:501	arg1	scaffolds					513:521	The prepared composite scaffolds	490:521	The prepared composite scaffolds	490:521	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	5	48	with	comparison	990:999	arg1	scaffolds					1038:1046	the pure CS and CS-OM composite scaffolds	1006:1046	the pure CS and CS-OM composite scaffolds	1006:1046	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	6	49	theme	CS-OM-HA	1049:1056	arg1	scaffolds					1068:1076	CS-OM-HA composite scaffolds	1049:1076	CS-OM-HA composite scaffolds	1049:1076	CS-OM-HA composite scaffolds were also found to be non-cytotoxic to MG 63 osteoblast cell lines.
26505953	2	50	theme	Hydroxyapatite	297:310	arg1	particles					317:325	Hydroxyapatite (HA) particles	297:325	Hydroxyapatite (HA) particles of size ∼ 65 nm	297:341	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	1	51	theme	foaming	281:287	arg1	method					289:294	gas foaming method	277:294	gas foaming method	277:294	In this study, a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold was developed by combining microwave irradiation and gas foaming method.
26505953	2	52	dep	spectroscopy	476:487	arg1	transform					446:454	transform	446:454	transform	446:454	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	1	53	theme	modified	154:161	arg1	scaffold					215:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	In this study, a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold was developed by combining microwave irradiation and gas foaming method.
26505953	0	54	theme	composite	88:96	arg1	scaffolds					98:106	montmorillonite-hydroxyapatite composite scaffolds	57:106	montmorillonite-hydroxyapatite composite scaffolds	57:106	Microwave-assisted synthesis of porous chitosan-modified montmorillonite-hydroxyapatite composite scaffolds.
26505953	6	55	theme	MG	1117:1118	arg1	lines					1139:1143	MG 63 osteoblast cell lines	1117:1143	MG 63 osteoblast cell lines	1117:1143	CS-OM-HA composite scaffolds were also found to be non-cytotoxic to MG 63 osteoblast cell lines.
26505953	1	56	theme	montmorillonite-hydroxyapatite	163:192	arg1	scaffold					215:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	In this study, a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold was developed by combining microwave irradiation and gas foaming method.
26505953	7	57	theme	novel	1191:1195	arg1	scaffold					1216:1223	the novel CS-OM-HA composite scaffold	1187:1223	the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties	1187:1283	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	4	58	theme	biological	714:723	arg1	bioactivity					783:793	bioactivity	783:793	bioactivity	783:793	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	58	theme	biological	714:723	arg1	degradation					747:757	degradation	747:757	degradation	747:757	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	58	theme	biological	714:723	arg1	adsorption					768:777	protein adsorption	760:777	protein adsorption	760:777	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	58	theme	biological	714:723	arg1	swelling					737:744	swelling	737:744	swelling	737:744	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	58	theme	biological	714:723	arg1	properties					725:734	the mechanical and in vitro biological properties	686:734	the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds	686:821	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	2	59	theme	X-ray	381:385	arg1	diffraction					387:397	X-ray diffraction	381:397	X-ray diffraction (XRD)	381:403	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	2	59	theme	X-ray	381:385	arg1	XRD					400:402	XRD	400:402	XRD	400:402	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	3	60	theme	electron	612:619	arg1	microscopy					621:630	scanning electron microscopy	603:630	scanning electron microscopy (SEM) studies	603:644	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	3	60	theme	electron	612:619	arg1	SEM					633:635	SEM	633:635	SEM	633:635	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	4	61	theme	composite	803:811	arg1	scaffolds					813:821	the composite scaffolds	799:821	the composite scaffolds	799:821	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	7	62	theme	tissue	1329:1334	arg1	applications					1348:1359	bone tissue engineering applications	1324:1359	bone tissue engineering applications	1324:1359	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	4	63	theme	protein	760:766	arg1	adsorption					768:777	protein adsorption	760:777	protein adsorption	760:777	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	63	theme	protein	760:766	arg1	properties					725:734	the mechanical and in vitro biological properties	686:734	the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds	686:821	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	7	64	theme	in	1254:1255	arg1	properties					1274:1283	improved mechanical and in vitro biological properties	1230:1283	improved mechanical and in vitro biological properties	1230:1283	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	1	65	theme	CS-OM-HA	195:202	arg1	scaffold					215:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	In this study, a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold was developed by combining microwave irradiation and gas foaming method.
26505953	3	66	theme	ATR-FTIR	548:555	arg1	XRD					558:560	XRD	558:560	XRD	558:560	The prepared composite scaffolds were characterized using ATR-FTIR, XRD, mercury intrusion porosimeter (MIP) and scanning electron microscopy (SEM) studies.
26505953	7	67	theme	biological	1263:1272	arg1	properties					1274:1283	improved mechanical and in vitro biological properties	1230:1283	improved mechanical and in vitro biological properties	1230:1283	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	5	68	theme	CS-OM	1022:1026	arg1	scaffolds					1038:1046	the pure CS and CS-OM composite scaffolds	1006:1046	the pure CS and CS-OM composite scaffolds	1006:1046	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	5	69	theme	property	873:880	arg1	studies					918:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies	839:924	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds	839:956	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	2	70	theme	attenuated	409:418	arg1	spectroscopy					476:487	infrared (ATR-FTIR) spectroscopy	456:487	attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy	409:487	Hydroxyapatite (HA) particles of size ∼ 65 nm were synthesized and characterized by X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy.
26505953	4	71	theme	mechanical	690:699	arg1	bioactivity					783:793	bioactivity	783:793	bioactivity	783:793	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	71	theme	mechanical	690:699	arg1	degradation					747:757	degradation	747:757	degradation	747:757	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	71	theme	mechanical	690:699	arg1	adsorption					768:777	protein adsorption	760:777	protein adsorption	760:777	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	71	theme	mechanical	690:699	arg1	swelling					737:744	swelling	737:744	swelling	737:744	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	71	theme	mechanical	690:699	arg1	properties					725:734	the mechanical and in vitro biological properties	686:734	the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds	686:821	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	4	72	theme	HA	673:674	arg1	effect					663:668	The synergistic effect	647:668	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds	647:821	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	7	73	with	scaffold	1216:1223	arg1	properties					1274:1283	improved mechanical and in vitro biological properties	1230:1283	improved mechanical and in vitro biological properties	1230:1283	From the study, it can be concluded that the novel CS-OM-HA composite scaffold with improved mechanical and in vitro biological properties has wide potential in non-load bearing bone tissue engineering applications.
26505953	5	74	theme	mechanical	862:871	arg1	property					873:880	mechanical property	862:880	mechanical property	862:880	Swelling, degradation, mechanical property, bioactivity and protein adsorption studies of CS-OM-HA composite scaffolds have shown desirable results in comparison with the pure CS and CS-OM composite scaffolds.
26505953	4	75	theme	synergistic	651:661	arg1	effect					663:668	The synergistic effect	647:668	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds	647:821	The synergistic effect of HA and OM on the mechanical and in vitro biological properties (swelling, degradation, protein adsorption and bioactivity) of the composite scaffolds were evaluated.
26505953	1	76	theme	composite	205:213	arg1	scaffold					215:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold	124:222	In this study, a porous chitosan-organically modified montmorillonite-hydroxyapatite (CS-OM-HA) composite scaffold was developed by combining microwave irradiation and gas foaming method.
27615746	3	0	theme	copolymer-functionalized	568:591	arg1	CNCs					593:596	copolymer-functionalized CNCs	568:596	copolymer-functionalized CNCs	568:596	The composition of the copolymer was tuned to achieve gelation of the suspension of copolymer-functionalized CNCs at 37 °C in cell culture medium and gel dissociation upon cooling it to room temperature.
27615746	6	1	theme	further	1066:1072	arg1	characterization					1079:1094	further cell characterization	1066:1094	further cell characterization	1066:1094	Following cell culture, the cells were released from the gel by reducing the temperature, thus, enabling further cell characterization.
27615746	1	2	theme	artificial	169:178	arg1	matrices					194:201	artificial extracellular matrices	169:201	artificial extracellular matrices	169:201	Natural extracellular matrices often have a filamentous nature, however, only a limited number of artificial extracellular matrices have been designed from nanofibrillar building blocks.
27615746	8	3	theme	different	1339:1347	arg1	medium					1362:1367	a different cell culture medium	1337:1367	a different cell culture medium	1337:1367	The release of cells following their culture in the hydrogels would enable enhanced cell characterization and potential transfer in a different cell culture medium.
27615746	7	4	theme	nanofibrillar	1180:1192	arg1	hydrogels					1194:1202	injectable temperature-responsive nanofibrillar hydrogels	1146:1202	injectable temperature-responsive nanofibrillar hydrogels	1146:1202	These results pave the way for the generation of injectable temperature-responsive nanofibrillar hydrogels.
27615746	1	5	theme	extracellular	79:91	arg1	matrices					93:100	Natural extracellular matrices	71:100	Natural extracellular matrices	71:100	Natural extracellular matrices often have a filamentous nature, however, only a limited number of artificial extracellular matrices have been designed from nanofibrillar building blocks.
27615746	2	6	from	nanocrystals	365:376	arg1	preparation					277:287	the preparation	273:287	the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate	273:481	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	4	7	theme	mass	823:826	arg1	ratio					828:832	the CNC-to-copolymer mass ratio	802:832	the CNC-to-copolymer mass ratio	802:832	The mechanical properties and the structure of the hydrogel were controlled by changing copolymer composition and the CNC-to-copolymer mass ratio.
27615746	3	8	theme	culture	615:621	arg1	medium					623:628	cell culture medium	610:628	cell culture medium	610:628	The composition of the copolymer was tuned to achieve gelation of the suspension of copolymer-functionalized CNCs at 37 °C in cell culture medium and gel dissociation upon cooling it to room temperature.
27615746	2	9	theme	hydrogels	329:337	arg1	preparation					277:287	the preparation	273:287	the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate	273:481	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	8	10	theme	cells	1220:1224	arg1	release					1209:1215	The release	1205:1215	The release of cells following their culture in the hydrogels	1205:1265	The release of cells following their culture in the hydrogels would enable enhanced cell characterization and potential transfer in a different cell culture medium.
27615746	5	11	theme	T	924:924	arg1	cells					926:930	T cells	924:930	T cells	924:930	The thermoreversible gels were used for the encapsulation and culture of fibroblasts and T cells and showed low cytotoxicity.
27615746	4	12	theme	mechanical	692:701	arg1	properties					703:712	The mechanical properties	688:712	The mechanical properties	688:712	The mechanical properties and the structure of the hydrogel were controlled by changing copolymer composition and the CNC-to-copolymer mass ratio.
27615746	2	13	theme	nanofibrillar	315:327	arg1	hydrogels					329:337	temperature-responsive nanofibrillar hydrogels	292:337	temperature-responsive nanofibrillar hydrogels	292:337	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	4	14	theme	hydrogel	739:746	arg1	properties					703:712	The mechanical properties	688:712	The mechanical properties	688:712	The mechanical properties and the structure of the hydrogel were controlled by changing copolymer composition and the CNC-to-copolymer mass ratio.
27615746	4	14	theme	hydrogel	739:746	arg1	structure					722:730	the structure	718:730	the structure of the hydrogel	718:746	The mechanical properties and the structure of the hydrogel were controlled by changing copolymer composition and the CNC-to-copolymer mass ratio.
27615746	1	15	theme	extracellular	180:192	arg1	matrices					194:201	artificial extracellular matrices	169:201	artificial extracellular matrices	169:201	Natural extracellular matrices often have a filamentous nature, however, only a limited number of artificial extracellular matrices have been designed from nanofibrillar building blocks.
27615746	6	16	theme	cell	971:974	arg1	culture					976:982	cell culture	971:982	cell culture	971:982	Following cell culture, the cells were released from the gel by reducing the temperature, thus, enabling further cell characterization.
27615746	5	17	dep	encapsulation	879:891	arg1	the					875:877	the	875:877	the	875:877	The thermoreversible gels were used for the encapsulation and culture of fibroblasts and T cells and showed low cytotoxicity.
27615746	2	18	theme	temperature-responsive	292:313	arg1	hydrogels					329:337	temperature-responsive nanofibrillar hydrogels	292:337	temperature-responsive nanofibrillar hydrogels	292:337	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	1	19	theme	matrices	194:201	arg1	number					159:164	only a limited number	144:164	only a limited number of artificial extracellular matrices	144:201	Natural extracellular matrices often have a filamentous nature, however, only a limited number of artificial extracellular matrices have been designed from nanofibrillar building blocks.
27615746	0	20	theme	Nanofibrillar	23:35	arg1	Hydrogels					37:45	Temperature-Responsive Nanofibrillar Hydrogels	0:45	Temperature-Responsive Nanofibrillar Hydrogels for Cell Encapsulation.	0:69	Temperature-Responsive Nanofibrillar Hydrogels for Cell Encapsulation.
27615746	7	21	theme	injectable	1146:1155	arg1	hydrogels					1194:1202	injectable temperature-responsive nanofibrillar hydrogels	1146:1202	injectable temperature-responsive nanofibrillar hydrogels	1146:1202	These results pave the way for the generation of injectable temperature-responsive nanofibrillar hydrogels.
27615746	3	22	theme	gel	634:636	arg1	dissociation					638:649	gel dissociation	634:649	gel dissociation	634:649	The composition of the copolymer was tuned to achieve gelation of the suspension of copolymer-functionalized CNCs at 37 °C in cell culture medium and gel dissociation upon cooling it to room temperature.
27615746	2	23	theme	N	446:446	arg1	copolymer					407:415	a copolymer	405:415	a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate	405:481	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	0	24	theme	Temperature-Responsive	0:21	arg1	Hydrogels					37:45	Temperature-Responsive Nanofibrillar Hydrogels	0:45	Temperature-Responsive Nanofibrillar Hydrogels for Cell Encapsulation.	0:69	Temperature-Responsive Nanofibrillar Hydrogels for Cell Encapsulation.
27615746	1	25	contain	have	108:111	arg2	nature					127:132	a filamentous nature	113:132	a filamentous nature	113:132	Natural extracellular matrices often have a filamentous nature, however, only a limited number of artificial extracellular matrices have been designed from nanofibrillar building blocks.
27615746	1	25	contain	have	108:111	arg1	matrices					93:100	Natural extracellular matrices	71:100	Natural extracellular matrices	71:100	Natural extracellular matrices often have a filamentous nature, however, only a limited number of artificial extracellular matrices have been designed from nanofibrillar building blocks.
27615746	7	26	theme	temperature-responsive	1157:1178	arg1	hydrogels					1194:1202	injectable temperature-responsive nanofibrillar hydrogels	1146:1202	injectable temperature-responsive nanofibrillar hydrogels	1146:1202	These results pave the way for the generation of injectable temperature-responsive nanofibrillar hydrogels.
27615746	1	27	theme	filamentous	115:125	arg1	nature					127:132	a filamentous nature	113:132	a filamentous nature	113:132	Natural extracellular matrices often have a filamentous nature, however, only a limited number of artificial extracellular matrices have been designed from nanofibrillar building blocks.
27615746	8	28	theme	cell	1349:1352	arg1	medium					1362:1367	a different cell culture medium	1337:1367	a different cell culture medium	1337:1367	The release of cells following their culture in the hydrogels would enable enhanced cell characterization and potential transfer in a different cell culture medium.
27615746	2	29	theme	cellulose	355:363	arg1	CNCs					379:382	CNCs	379:382	CNCs	379:382	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	2	29	theme	cellulose	355:363	arg1	nanocrystals					365:376	rod-shaped cellulose nanocrystals	344:376	rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate	344:481	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	4	30	theme	CNC-to-copolymer	806:821	arg1	ratio					828:832	the CNC-to-copolymer mass ratio	802:832	the CNC-to-copolymer mass ratio	802:832	The mechanical properties and the structure of the hydrogel were controlled by changing copolymer composition and the CNC-to-copolymer mass ratio.
27615746	8	31	from	culture	1242:1248	arg1	hydrogels					1257:1265	the hydrogels	1253:1265	the hydrogels	1253:1265	The release of cells following their culture in the hydrogels would enable enhanced cell characterization and potential transfer in a different cell culture medium.
27615746	2	32	theme	rod-shaped	344:353	arg1	CNCs					379:382	CNCs	379:382	CNCs	379:382	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	2	32	theme	rod-shaped	344:353	arg1	nanocrystals					365:376	rod-shaped cellulose nanocrystals	344:376	rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate	344:481	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	1	33	theme	limited	151:157	arg1	number					159:164	only a limited number	144:164	only a limited number of artificial extracellular matrices	144:201	Natural extracellular matrices often have a filamentous nature, however, only a limited number of artificial extracellular matrices have been designed from nanofibrillar building blocks.
27615746	8	34	theme	potential	1315:1323	arg1	transfer					1325:1332	potential transfer	1315:1332	potential transfer	1315:1332	The release of cells following their culture in the hydrogels would enable enhanced cell characterization and potential transfer in a different cell culture medium.
27615746	5	35	theme	cells	926:930	arg1	culture					897:903	culture	897:903	culture	897:903	The thermoreversible gels were used for the encapsulation and culture of fibroblasts and T cells and showed low cytotoxicity.
27615746	5	35	theme	cells	926:930	arg1	encapsulation					879:891	encapsulation	879:891	encapsulation	879:891	The thermoreversible gels were used for the encapsulation and culture of fibroblasts and T cells and showed low cytotoxicity.
27615746	8	36	theme	cell	1289:1292	arg1	characterization					1294:1309	enhanced cell characterization	1280:1309	enhanced cell characterization	1280:1309	The release of cells following their culture in the hydrogels would enable enhanced cell characterization and potential transfer in a different cell culture medium.
27615746	1	37	theme	nanofibrillar	227:239	arg1	blocks					250:255	nanofibrillar building blocks	227:255	nanofibrillar building blocks	227:255	Natural extracellular matrices often have a filamentous nature, however, only a limited number of artificial extracellular matrices have been designed from nanofibrillar building blocks.
27615746	2	38	theme	N'-dimethylaminoethyl	448:468	arg1	methacrylate					470:481	N'-dimethylaminoethyl methacrylate	448:481	N'-dimethylaminoethyl methacrylate	448:481	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	2	38	theme	N'-dimethylaminoethyl	448:468	arg1	N-isopropylacrylamide					420:440	N-isopropylacrylamide	420:440	N-isopropylacrylamide	420:440	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	5	39	theme	low	943:945	arg1	cytotoxicity					947:958	low cytotoxicity	943:958	low cytotoxicity	943:958	The thermoreversible gels were used for the encapsulation and culture of fibroblasts and T cells and showed low cytotoxicity.
27615746	0	40	theme	Cell	51:54	arg1	Encapsulation					56:68	Cell Encapsulation	51:68	Cell Encapsulation	51:68	Temperature-Responsive Nanofibrillar Hydrogels for Cell Encapsulation.
27615746	1	41	theme	building	241:248	arg1	blocks					250:255	nanofibrillar building blocks	227:255	nanofibrillar building blocks	227:255	Natural extracellular matrices often have a filamentous nature, however, only a limited number of artificial extracellular matrices have been designed from nanofibrillar building blocks.
27615746	5	42	theme	fibroblasts	908:918	arg1	culture					897:903	culture	897:903	culture	897:903	The thermoreversible gels were used for the encapsulation and culture of fibroblasts and T cells and showed low cytotoxicity.
27615746	5	42	theme	fibroblasts	908:918	arg1	encapsulation					879:891	encapsulation	879:891	encapsulation	879:891	The thermoreversible gels were used for the encapsulation and culture of fibroblasts and T cells and showed low cytotoxicity.
27615746	3	43	theme	copolymer	507:515	arg1	composition					488:498	The composition	484:498	The composition of the copolymer	484:515	The composition of the copolymer was tuned to achieve gelation of the suspension of copolymer-functionalized CNCs at 37 °C in cell culture medium and gel dissociation upon cooling it to room temperature.
27615746	3	44	theme	suspension	554:563	arg1	gelation					538:545	gelation	538:545	gelation of the suspension of copolymer-functionalized CNCs	538:596	The composition of the copolymer was tuned to achieve gelation of the suspension of copolymer-functionalized CNCs at 37 °C in cell culture medium and gel dissociation upon cooling it to room temperature.
27615746	5	45	used	used	866:869	arg2	gels					856:859	The thermoreversible gels	835:859	The thermoreversible gels	835:859	The thermoreversible gels were used for the encapsulation and culture of fibroblasts and T cells and showed low cytotoxicity.
27615746	7	46	theme	hydrogels	1194:1202	arg1	generation					1132:1141	the generation	1128:1141	the generation of injectable temperature-responsive nanofibrillar hydrogels	1128:1202	These results pave the way for the generation of injectable temperature-responsive nanofibrillar hydrogels.
27615746	3	47	theme	CNCs	593:596	arg1	suspension					554:563	the suspension	550:563	the suspension of copolymer-functionalized CNCs	550:596	The composition of the copolymer was tuned to achieve gelation of the suspension of copolymer-functionalized CNCs at 37 °C in cell culture medium and gel dissociation upon cooling it to room temperature.
27615746	4	48	theme	copolymer	776:784	arg1	composition					786:796	copolymer composition	776:796	copolymer composition	776:796	The mechanical properties and the structure of the hydrogel were controlled by changing copolymer composition and the CNC-to-copolymer mass ratio.
27615746	8	49	theme	culture	1354:1360	arg1	medium					1362:1367	a different cell culture medium	1337:1367	a different cell culture medium	1337:1367	The release of cells following their culture in the hydrogels would enable enhanced cell characterization and potential transfer in a different cell culture medium.
27615746	3	50	theme	cell	610:613	arg1	medium					623:628	cell culture medium	610:628	cell culture medium	610:628	The composition of the copolymer was tuned to achieve gelation of the suspension of copolymer-functionalized CNCs at 37 °C in cell culture medium and gel dissociation upon cooling it to room temperature.
27615746	3	51	theme	room	670:673	arg1	temperature					675:685	room temperature	670:685	room temperature	670:685	The composition of the copolymer was tuned to achieve gelation of the suspension of copolymer-functionalized CNCs at 37 °C in cell culture medium and gel dissociation upon cooling it to room temperature.
27615746	2	52	theme	N-isopropylacrylamide	420:440	arg1	copolymer					407:415	a copolymer	405:415	a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate	405:481	Here we report the preparation of temperature-responsive nanofibrillar hydrogels from rod-shaped cellulose nanocrystals (CNCs) functionalized with a copolymer of N-isopropylacrylamide and N,N'-dimethylaminoethyl methacrylate.
27615746	3	53	from	dissociation	638:649	arg1	medium					623:628	cell culture medium	610:628	cell culture medium	610:628	The composition of the copolymer was tuned to achieve gelation of the suspension of copolymer-functionalized CNCs at 37 °C in cell culture medium and gel dissociation upon cooling it to room temperature.
27615746	5	54	theme	thermoreversible	839:854	arg1	gels					856:859	The thermoreversible gels	835:859	The thermoreversible gels	835:859	The thermoreversible gels were used for the encapsulation and culture of fibroblasts and T cells and showed low cytotoxicity.
27615746	6	55	attach	released	1000:1007	arg1	gel					1018:1020	the gel	1014:1020	the gel	1014:1020	Following cell culture, the cells were released from the gel by reducing the temperature, thus, enabling further cell characterization.
27615746	6	55	attach	released	1000:1007	arg2	cells					989:993	the cells	985:993	the cells	985:993	Following cell culture, the cells were released from the gel by reducing the temperature, thus, enabling further cell characterization.
27615746	1	56	theme	Natural	71:77	arg1	matrices					93:100	Natural extracellular matrices	71:100	Natural extracellular matrices	71:100	Natural extracellular matrices often have a filamentous nature, however, only a limited number of artificial extracellular matrices have been designed from nanofibrillar building blocks.
27615746	8	57	theme	enhanced	1280:1287	arg1	characterization					1294:1309	enhanced cell characterization	1280:1309	enhanced cell characterization	1280:1309	The release of cells following their culture in the hydrogels would enable enhanced cell characterization and potential transfer in a different cell culture medium.
27615746	3	58	from	°C	604:605	arg1	medium					623:628	cell culture medium	610:628	cell culture medium	610:628	The composition of the copolymer was tuned to achieve gelation of the suspension of copolymer-functionalized CNCs at 37 °C in cell culture medium and gel dissociation upon cooling it to room temperature.
27615746	6	59	theme	cell	1074:1077	arg1	characterization					1079:1094	further cell characterization	1066:1094	further cell characterization	1066:1094	Following cell culture, the cells were released from the gel by reducing the temperature, thus, enabling further cell characterization.
26566174	8	0	theme	cell	1246:1249	arg1	attachment					1251:1260	cell attachment	1246:1260	cell attachment	1246:1260	Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin-only scaffolds.
26566174	3	1	theme	standard	571:578	arg1	set-up					596:601	a standard electrospinning set-up	569:601	a standard electrospinning set-up	569:601	In this research, a composite structured nano-/microfibrous scaffold, made from a mixture of chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up was developed.
26566174	6	2	theme	significant	996:1006	arg1	increase					1008:1015	significant increase	996:1015	significant increase in mean pore size and its distribution	996:1054	Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution.
26566174	10	3	theme	tissue	1699:1704	arg1	engineering					1706:1716	cell-based tissue engineering	1688:1716	cell-based tissue engineering	1688:1716	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	11	4	theme	mesoporous	1805:1814	arg1	scaffolds					1854:1862	mesoporous composite chitosan-GP-gelatin fibrous scaffolds	1805:1862	mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics	1805:1949	These results suggested the potential of using mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics.
26566174	8	5	theme	proliferation	1274:1286	arg1	property					1288:1295	better proliferation property	1267:1295	better proliferation property	1267:1295	Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin-only scaffolds.
26566174	6	6	theme	polymer	970:976	arg1	blend					978:982	the polymer blend	966:982	the polymer blend	966:982	Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution.
26566174	10	7	theme	fabricated	1522:1531	arg1	scaffold					1574:1581	a biologically compatible scaffold	1548:1581	a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices	1548:1755	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	10	7	theme	fabricated	1522:1531	arg1	biomaterial					1533:1543	the fabricated biomaterial	1518:1543	the fabricated biomaterial	1518:1543	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	2	8	theme	hard	354:357	arg1	handling					359:366	their hard handling	348:366	their hard handling	348:366	Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling remains major challenges to be addressed.
26566174	1	9	theme	regenerative	229:240	arg1	medicine					242:249	the regenerative medicine	225:249	the regenerative medicine	225:249	Scaffold-based tissue engineering is considered as a promising approach in the regenerative medicine.
26566174	7	10	theme	control	1162:1168	arg1	groups					1170:1175	the test and control groups	1149:1175	groups	1170:1175	Interaction between the scaffold and the contained cells was also monitored and compared in the test and control groups.
26566174	9	11	theme	fabricated	1340:1349	arg1	scaffolds					1351:1359	The fabricated scaffolds	1336:1359	The fabricated scaffolds	1336:1359	The fabricated scaffolds, unlike many other natural polymers, also exhibit non-toxic and biodegradable properties in the grafted tissues.
26566174	7	12	theme	test	1153:1156	arg1	groups					1170:1175	the test and control groups	1149:1175	groups	1170:1175	Interaction between the scaffold and the contained cells was also monitored and compared in the test and control groups.
26566174	10	13	theme	healing	1739:1745	arg1	practices					1747:1755	wound healing practices	1733:1755	wound healing practices	1733:1755	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	9	14	theme	many	1369:1372	arg1	polymers					1388:1395	many other natural polymers	1369:1395	many other natural polymers	1369:1395	The fabricated scaffolds, unlike many other natural polymers, also exhibit non-toxic and biodegradable properties in the grafted tissues.
26566174	7	15	theme	contained	1098:1106	arg1	cells					1108:1112	the contained cells	1094:1112	the contained cells	1094:1112	Interaction between the scaffold and the contained cells was also monitored and compared in the test and control groups.
26566174	1	16	theme	tissue	165:170	arg1	engineering					172:182	Scaffold-based tissue engineering	150:182	Scaffold-based tissue engineering	150:182	Scaffold-based tissue engineering is considered as a promising approach in the regenerative medicine.
26566174	1	16	theme	tissue	165:170	arg1	approach					213:220	a promising approach	201:220	a promising approach in the regenerative medicine	201:249	Scaffold-based tissue engineering is considered as a promising approach in the regenerative medicine.
26566174	6	17	theme	pore	1025:1028	arg1	size					1030:1033	mean pore size	1020:1033	mean pore size	1020:1033	Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution.
26566174	11	18	theme	cell	1930:1933	arg1	characteristics					1935:1949	different inherent cell characteristics	1911:1949	different inherent cell characteristics	1911:1949	These results suggested the potential of using mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics.
26566174	9	19	theme	natural	1380:1386	arg1	polymers					1388:1395	many other natural polymers	1369:1395	many other natural polymers	1369:1395	The fabricated scaffolds, unlike many other natural polymers, also exhibit non-toxic and biodegradable properties in the grafted tissues.
26566174	2	20	theme	mechanical	299:308	arg1	properties					310:319	poor mechanical properties	294:319	poor mechanical properties	294:319	Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling remains major challenges to be addressed.
26566174	4	21	theme	mechanical	749:758	arg1	properties					775:784	their mechanical and biological properties	743:784	their mechanical and biological properties	743:784	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions were prepared at ratios of 30/70, 50/50, 70/30 (w/w) and their mechanical and biological properties were engineered.
26566174	11	22	theme	different	1911:1919	arg1	characteristics					1935:1949	different inherent cell characteristics	1911:1949	different inherent cell characteristics	1911:1949	These results suggested the potential of using mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics.
26566174	0	23	theme	mechanical	89:98	arg1	properties					115:124	suitable mechanical and biological properties	80:124	suitable mechanical and biological properties for tissue engineering	80:147	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.
26566174	1	24	from	approach	213:220	arg1	medicine					242:249	the regenerative medicine	225:249	the regenerative medicine	225:249	Scaffold-based tissue engineering is considered as a promising approach in the regenerative medicine.
26566174	4	25	theme	biological	764:773	arg1	properties					775:784	their mechanical and biological properties	743:784	their mechanical and biological properties	743:784	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions were prepared at ratios of 30/70, 50/50, 70/30 (w/w) and their mechanical and biological properties were engineered.
26566174	0	26	theme	biological	104:113	arg1	properties					115:124	suitable mechanical and biological properties	80:124	suitable mechanical and biological properties for tissue engineering	80:147	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.
26566174	1	27	theme	promising	203:211	arg1	engineering					172:182	Scaffold-based tissue engineering	150:182	Scaffold-based tissue engineering	150:182	Scaffold-based tissue engineering is considered as a promising approach in the regenerative medicine.
26566174	1	27	theme	promising	203:211	arg1	approach					213:220	a promising approach	201:220	a promising approach in the regenerative medicine	201:249	Scaffold-based tissue engineering is considered as a promising approach in the regenerative medicine.
26566174	5	28	theme	pore	820:823	arg1	structure					825:833	the pore structure	816:833	the pore structure of the fabricated nanofibrous scaffolds	816:873	Furthermore, the pore structure of the fabricated nanofibrous scaffolds was investigated and predicted using a theoretical model.
26566174	10	29	with	scaffold	1574:1581	arg1	potential					1588:1596	potential	1588:1596	potential	1588:1596	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	0	30	theme	Hybrid	0:5	arg1	scaffolds					65:73	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds	0:73	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.	0:148	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.
26566174	4	31	theme	acetic	631:636	arg1	solutions					676:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions	618:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions	618:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions were prepared at ratios of 30/70, 50/50, 70/30 (w/w) and their mechanical and biological properties were engineered.
26566174	3	32	theme	chitosan-ß-glycerol	503:521	arg1	chitosan-GP-gelatin					542:560	chitosan-GP-gelatin	542:560	chitosan-GP-gelatin	542:560	In this research, a composite structured nano-/microfibrous scaffold, made from a mixture of chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up was developed.
26566174	3	32	theme	chitosan-ß-glycerol	503:521	arg1	phosphate-gelatin					523:539	chitosan-ß-glycerol phosphate-gelatin	503:539	chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up	503:601	In this research, a composite structured nano-/microfibrous scaffold, made from a mixture of chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up was developed.
26566174	0	33	theme	phosphate-gelatin	27:43	arg1	scaffolds					65:73	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds	0:73	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.	0:148	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.
26566174	3	34	theme	nano-/microfibrous	451:468	arg1	scaffold					470:477	a composite structured nano-/microfibrous scaffold	428:477	a composite structured nano-/microfibrous scaffold	428:477	In this research, a composite structured nano-/microfibrous scaffold, made from a mixture of chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up was developed.
26566174	4	35	theme	chitosan	642:649	arg1	solutions					676:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions	618:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions	618:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions were prepared at ratios of 30/70, 50/50, 70/30 (w/w) and their mechanical and biological properties were engineered.
26566174	0	36	theme	fibrous	57:63	arg1	scaffolds					65:73	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds	0:73	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.	0:148	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.
26566174	5	37	theme	scaffolds	865:873	arg1	structure					825:833	the pore structure	816:833	the pore structure of the fabricated nanofibrous scaffolds	816:873	Furthermore, the pore structure of the fabricated nanofibrous scaffolds was investigated and predicted using a theoretical model.
26566174	10	38	from	application	1673:1683	arg1	engineering					1706:1716	cell-based tissue engineering	1688:1716	cell-based tissue engineering	1688:1716	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	3	39	theme	composite	430:438	arg1	scaffold					470:477	a composite structured nano-/microfibrous scaffold	428:477	a composite structured nano-/microfibrous scaffold	428:477	In this research, a composite structured nano-/microfibrous scaffold, made from a mixture of chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up was developed.
26566174	10	40	theme	cultured	1646:1653	arg1	cells					1655:1659	the cultured cells	1642:1659	the cultured cells for further application in cell-based tissue engineering	1642:1716	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	11	41	theme	fibrous	1846:1852	arg1	scaffolds					1854:1862	mesoporous composite chitosan-GP-gelatin fibrous scaffolds	1805:1862	mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics	1805:1949	These results suggested the potential of using mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics.
26566174	8	42	theme	higher	1193:1198	arg1	concentrations					1209:1222	higher chitosan concentrations	1193:1222	higher chitosan concentrations	1193:1222	Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin-only scaffolds.
26566174	6	43	theme	Higher	933:938	arg1	concentrations					948:961	Higher gelatin concentrations	933:961	Higher gelatin concentrations in the polymer blend	933:982	Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution.
26566174	2	44	theme	Graft	252:256	arg1	instability					258:268	Graft instability	252:268	Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling	252:366	Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling remains major challenges to be addressed.
26566174	2	45	theme	major	376:380	arg1	challenges					382:391	major challenges	376:391	major challenges	376:391	Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling remains major challenges to be addressed.
26566174	6	46	from	increase	1008:1015	arg1	size					1030:1033	mean pore size	1020:1033	mean pore size	1020:1033	Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution.
26566174	6	46	from	increase	1008:1015	arg1	distribution					1043:1054	its distribution	1039:1054	its distribution	1039:1054	Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution.
26566174	11	47	theme	three-dimensional	1880:1896	arg1	tissues					1898:1904	three-dimensional tissues	1880:1904	three-dimensional tissues	1880:1904	These results suggested the potential of using mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics.
26566174	3	48	theme	electrospinning	580:594	arg1	set-up					596:601	a standard electrospinning set-up	569:601	a standard electrospinning set-up	569:601	In this research, a composite structured nano-/microfibrous scaffold, made from a mixture of chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up was developed.
26566174	8	49	theme	higher	1231:1236	arg1	rate					1238:1241	higher rate	1231:1241	higher rate of cell attachment	1231:1260	Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin-only scaffolds.
26566174	6	50	theme	mean	1020:1023	arg1	size					1030:1033	mean pore size	1020:1033	mean pore size	1020:1033	Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution.
26566174	6	51	from	concentrations	948:961	arg1	blend					978:982	the polymer blend	966:982	the polymer blend	966:982	Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution.
26566174	4	52	theme	phosphate-HCL	662:674	arg1	solutions					676:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions	618:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions	618:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions were prepared at ratios of 30/70, 50/50, 70/30 (w/w) and their mechanical and biological properties were engineered.
26566174	8	53	theme	attachment	1251:1260	arg1	rate					1238:1241	higher rate	1231:1241	higher rate of cell attachment	1231:1260	Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin-only scaffolds.
26566174	10	54	theme	cell-based	1688:1697	arg1	engineering					1706:1716	cell-based tissue engineering	1688:1716	cell-based tissue engineering	1688:1716	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	11	55	theme	composite	1816:1824	arg1	scaffolds					1854:1862	mesoporous composite chitosan-GP-gelatin fibrous scaffolds	1805:1862	mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics	1805:1949	These results suggested the potential of using mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics.
26566174	2	56	theme	rapid	325:329	arg1	degradation					331:341	rapid degradation	325:341	rapid degradation	325:341	Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling remains major challenges to be addressed.
26566174	8	57	theme	better	1267:1272	arg1	property					1288:1295	better proliferation property	1267:1295	better proliferation property	1267:1295	Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin-only scaffolds.
26566174	10	58	theme	wound	1733:1737	arg1	healing					1739:1745	wound healing	1733:1745	wound healing practices	1733:1755	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	5	59	theme	fabricated	842:851	arg1	scaffolds					865:873	the fabricated nanofibrous scaffolds	838:873	the fabricated nanofibrous scaffolds	838:873	Furthermore, the pore structure of the fabricated nanofibrous scaffolds was investigated and predicted using a theoretical model.
26566174	2	60	theme	handling	359:366	arg1	instability					258:268	Graft instability	252:268	Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling	252:366	Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling remains major challenges to be addressed.
26566174	5	61	theme	theoretical	914:924	arg1	model					926:930	a theoretical model	912:930	a theoretical model	912:930	Furthermore, the pore structure of the fabricated nanofibrous scaffolds was investigated and predicted using a theoretical model.
26566174	8	62	theme	gelatin-only	1312:1323	arg1	scaffolds					1325:1333	gelatin-only scaffolds	1312:1333	gelatin-only scaffolds	1312:1333	Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin-only scaffolds.
26566174	2	63	theme	collagen	273:280	arg1	instability					258:268	Graft instability	252:268	Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling	252:366	Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling remains major challenges to be addressed.
26566174	1	64	theme	Scaffold-based	150:163	arg1	engineering					172:182	Scaffold-based tissue engineering	150:182	Scaffold-based tissue engineering	150:182	Scaffold-based tissue engineering is considered as a promising approach in the regenerative medicine.
26566174	1	64	theme	Scaffold-based	150:163	arg1	approach					213:220	a promising approach	201:220	a promising approach in the regenerative medicine	201:249	Scaffold-based tissue engineering is considered as a promising approach in the regenerative medicine.
26566174	9	65	theme	other	1374:1378	arg1	polymers					1388:1395	many other natural polymers	1369:1395	many other natural polymers	1369:1395	The fabricated scaffolds, unlike many other natural polymers, also exhibit non-toxic and biodegradable properties in the grafted tissues.
26566174	2	66	theme	poor	294:297	arg1	properties					310:319	poor mechanical properties	294:319	poor mechanical properties	294:319	Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling remains major challenges to be addressed.
26566174	11	67	theme	inherent	1921:1928	arg1	characteristics					1935:1949	different inherent cell characteristics	1911:1949	different inherent cell characteristics	1911:1949	These results suggested the potential of using mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics.
26566174	10	68	theme	compatible	1563:1572	arg1	scaffold					1574:1581	a biologically compatible scaffold	1548:1581	a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices	1548:1755	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	10	68	theme	compatible	1563:1572	arg1	biomaterial					1533:1543	the fabricated biomaterial	1518:1543	the fabricated biomaterial	1518:1543	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	0	69	theme	tissue	130:135	arg1	engineering					137:147	tissue engineering	130:147	tissue engineering	130:147	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.
26566174	9	70	theme	non-toxic	1411:1419	arg1	properties					1439:1448	non-toxic and biodegradable properties	1411:1448	non-toxic and biodegradable properties	1411:1448	The fabricated scaffolds, unlike many other natural polymers, also exhibit non-toxic and biodegradable properties in the grafted tissues.
26566174	9	71	theme	biodegradable	1425:1437	arg1	properties					1439:1448	non-toxic and biodegradable properties	1411:1448	non-toxic and biodegradable properties	1411:1448	The fabricated scaffolds, unlike many other natural polymers, also exhibit non-toxic and biodegradable properties in the grafted tissues.
26566174	0	72	theme	chitosan-ß-glycerol	7:25	arg1	scaffolds					65:73	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds	0:73	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.	0:148	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.
26566174	0	73	theme	nano-/micro	45:55	arg1	scaffolds					65:73	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds	0:73	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.	0:148	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.
26566174	9	74	theme	grafted	1457:1463	arg1	tissues					1465:1471	the grafted tissues	1453:1471	the grafted tissues	1453:1471	The fabricated scaffolds, unlike many other natural polymers, also exhibit non-toxic and biodegradable properties in the grafted tissues.
26566174	4	75	theme	ß-glycerol	651:660	arg1	solutions					676:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions	618:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions	618:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions were prepared at ratios of 30/70, 50/50, 70/30 (w/w) and their mechanical and biological properties were engineered.
26566174	10	76	theme	proper	1612:1617	arg1	platform					1619:1626	a proper platform	1610:1626	a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices	1610:1755	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	3	77	theme	phosphate-gelatin	523:539	arg1	mixture					492:498	a mixture	490:498	a mixture of chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up	490:601	In this research, a composite structured nano-/microfibrous scaffold, made from a mixture of chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up was developed.
26566174	5	78	theme	nanofibrous	853:863	arg1	scaffolds					865:873	the fabricated nanofibrous scaffolds	838:873	the fabricated nanofibrous scaffolds	838:873	Furthermore, the pore structure of the fabricated nanofibrous scaffolds was investigated and predicted using a theoretical model.
26566174	11	79	theme	chitosan-GP-gelatin	1826:1844	arg1	scaffolds					1854:1862	mesoporous composite chitosan-GP-gelatin fibrous scaffolds	1805:1862	mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics	1805:1949	These results suggested the potential of using mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics.
26566174	0	80	theme	suitable	80:87	arg1	properties					115:124	suitable mechanical and biological properties	80:124	suitable mechanical and biological properties for tissue engineering	80:147	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.
26566174	8	81	theme	chitosan	1200:1207	arg1	concentrations					1209:1222	higher chitosan concentrations	1193:1222	higher chitosan concentrations	1193:1222	Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin-only scaffolds.
26566174	6	82	theme	gelatin	940:946	arg1	concentrations					948:961	Higher gelatin concentrations	933:961	Higher gelatin concentrations in the polymer blend	933:982	Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution.
26566174	3	83	theme	structured	440:449	arg1	scaffold					470:477	a composite structured nano-/microfibrous scaffold	428:477	a composite structured nano-/microfibrous scaffold	428:477	In this research, a composite structured nano-/microfibrous scaffold, made from a mixture of chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up was developed.
26566174	4	84	theme	Gelatin-acid	618:629	arg1	solutions					676:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions	618:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions	618:684	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions were prepared at ratios of 30/70, 50/50, 70/30 (w/w) and their mechanical and biological properties were engineered.
26566174	8	85	with	Scaffolds	1178:1186	arg1	concentrations					1209:1222	higher chitosan concentrations	1193:1222	higher chitosan concentrations	1193:1222	Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin-only scaffolds.
26566174	4	86	theme	30/70	713:717	arg1	ratios					703:708	ratios	703:708	ratios of 30/70, 50/50, 70/30 (w/w)	703:737	Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions were prepared at ratios of 30/70, 50/50, 70/30 (w/w) and their mechanical and biological properties were engineered.
26566174	10	87	theme	further	1665:1671	arg1	application					1673:1683	further application	1665:1683	further application in cell-based tissue engineering	1665:1716	In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices.
26566174	0	88	with	scaffolds	65:73	arg1	properties					115:124	suitable mechanical and biological properties	80:124	suitable mechanical and biological properties for tissue engineering	80:147	Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.
28549117	1	0	theme	useful	153:158	arg1	agglutinin					131:140	Wisteria floribunda agglutinin	111:140	Wisteria floribunda agglutinin (WFA)	111:146	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	1	0	theme	useful	153:158	arg1	probe					160:164	a useful probe	151:164	a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis	151:287	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	4	1	theme	glycoconjugate	805:818	arg1	microarray					820:829	glycoconjugate microarray	805:829	glycoconjugate microarray	805:829	We characterized the natural and rWFA by the glycoconjugate microarray and frontal affinity chromatography.
28549117	3	2	theme	complementary	476:488	arg1	sequence					494:501	The natural complementary DNA sequence	464:501	The natural complementary DNA sequence obtained from wisteria seeds	464:530	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	4	3	theme	frontal	835:841	arg1	chromatography					852:865	frontal affinity chromatography	835:865	frontal affinity chromatography	835:865	We characterized the natural and rWFA by the glycoconjugate microarray and frontal affinity chromatography.
28549117	3	4	theme	wisteria	517:524	arg1	seeds					526:530	wisteria seeds	517:530	wisteria seeds	517:530	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	1	5	theme	hepatic	272:278	arg1	fibrosis					280:287	hepatic fibrosis	272:287	hepatic fibrosis	272:287	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	5	6	theme	similar	910:916	arg1	specificity					898:908	glycan binding specificity	883:908	glycan binding specificity similar to that of natural WFA	883:939	rWFA exhibited glycan binding specificity similar to that of natural WFA: both bound to Gal- and N-acetylgalactosamine (GalNAc)-terminated glycans.
28549117	6	7	theme	monomeric	1110:1118	arg1	lectin					1120:1125	a recombinant monomeric lectin	1096:1125	a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc	1096:1242	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	6	8	theme	binding	1140:1146	arg1	specificity					1148:1158	limited binding specificity	1132:1158	limited binding specificity	1132:1158	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	6	9	from	WFA	1041:1043	arg1	Cys-272					1080:1086	Cys-272	1080:1086	Cys-272	1080:1086	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	3	10	theme	natural	468:474	arg1	sequence					494:501	The natural complementary DNA sequence	464:501	The natural complementary DNA sequence obtained from wisteria seeds	464:530	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	6	11	theme	limited	1132:1138	arg1	specificity					1148:1158	limited binding specificity	1132:1158	limited binding specificity	1132:1158	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	7	12	theme	sensitive	1299:1307	arg1	detection					1319:1327	highly sensitive biomarker detection	1292:1327	highly sensitive biomarker detection	1292:1327	Thus, this engineered lectin may be applied to highly sensitive biomarker detection.
28549117	3	13	from	C-terminus	692:701	arg1	peptide					645:651	a hydrophobic signal peptide	624:651	a hydrophobic signal peptide at the N-terminus	624:669	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	3	13	from	C-terminus	692:701	arg1	propeptide					674:683	a propeptide	672:683	a propeptide at the C-terminus	672:701	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	3	13	from	C-terminus	692:701	arg1	residue					731:737	a single cysteine (Cys) residue	707:737	a single cysteine (Cys) residue for dimer formation	707:757	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	6	14	theme	engineered	1030:1039	arg1	WFA					1041:1043	the engineered WFA	1026:1043	the engineered WFA with an amino acid substitution in Cys-272	1026:1086	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	3	15	theme	open	545:548	arg1	frame					558:562	an open reading frame	542:562	an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation	542:757	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	1	16	theme	glycan	185:190	arg1	alterations					203:213	glycan structural alterations	185:213	glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis	185:287	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	3	17	theme	single	709:714	arg1	residue					731:737	a single cysteine (Cys) residue	707:737	a single cysteine (Cys) residue for dimer formation	707:757	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	3	18	theme	reading	550:556	arg1	frame					558:562	an open reading frame	542:562	an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation	542:757	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	1	19	theme	structural	192:201	arg1	alterations					203:213	glycan structural alterations	185:213	glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis	185:287	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	0	20	theme	Wisteria	27:34	arg1	agglutinin					47:56	recombinant Wisteria floribunda agglutinin	15:56	recombinant Wisteria floribunda agglutinin	15:56	Engineering of recombinant Wisteria floribunda agglutinin specifically binding to GalNAcβ1,4GlcNAc (LacdiNAc).
28549117	3	21	contain	contained	532:540	arg2	frame					558:562	an open reading frame	542:562	an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation	542:757	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	3	21	contain	contained	532:540	arg1	sequence					494:501	The natural complementary DNA sequence	464:501	The natural complementary DNA sequence obtained from wisteria seeds	464:530	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	1	22	theme	Wisteria	111:118	arg1	WFA					143:145	WFA	143:145	WFA	143:145	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	1	22	theme	Wisteria	111:118	arg1	agglutinin					131:140	Wisteria floribunda agglutinin	111:140	Wisteria floribunda agglutinin (WFA)	111:146	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	1	22	theme	Wisteria	111:118	arg1	probe					160:164	a useful probe	151:164	a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis	151:287	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	0	23	theme	recombinant	15:25	arg1	agglutinin					47:56	recombinant Wisteria floribunda agglutinin	15:56	recombinant Wisteria floribunda agglutinin	15:56	Engineering of recombinant Wisteria floribunda agglutinin specifically binding to GalNAcβ1,4GlcNAc (LacdiNAc).
28549117	1	24	theme	floribunda	120:129	arg1	WFA					143:145	WFA	143:145	WFA	143:145	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	1	24	theme	floribunda	120:129	arg1	agglutinin					131:140	Wisteria floribunda agglutinin	111:140	Wisteria floribunda agglutinin (WFA)	111:146	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	1	24	theme	floribunda	120:129	arg1	probe					160:164	a useful probe	151:164	a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis	151:287	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	2	25	theme	recombinant	393:403	arg1	rWFA					410:413	rWFA	410:413	rWFA	410:413	Here, we identified the gene encoding WFA, and recombinant WFA (rWFA) was expressed in Escherichia coli and purified.
28549117	2	25	theme	recombinant	393:403	arg1	WFA					405:407	recombinant WFA	393:407	recombinant WFA (rWFA)	393:414	Here, we identified the gene encoding WFA, and recombinant WFA (rWFA) was expressed in Escherichia coli and purified.
28549117	0	26	theme	agglutinin	47:56	arg1	Engineering					0:10	Engineering	0:10	Engineering of recombinant Wisteria floribunda agglutinin	0:56	Engineering of recombinant Wisteria floribunda agglutinin specifically binding to GalNAcβ1,4GlcNAc (LacdiNAc).
28549117	7	27	theme	biomarker	1309:1317	arg1	detection					1319:1327	highly sensitive biomarker detection	1292:1327	highly sensitive biomarker detection	1292:1327	Thus, this engineered lectin may be applied to highly sensitive biomarker detection.
28549117	0	28	theme	floribunda	36:45	arg1	agglutinin					47:56	recombinant Wisteria floribunda agglutinin	15:56	recombinant Wisteria floribunda agglutinin	15:56	Engineering of recombinant Wisteria floribunda agglutinin specifically binding to GalNAcβ1,4GlcNAc (LacdiNAc).
28549117	3	29	from	N-terminus	660:669	arg1	peptide					645:651	a hydrophobic signal peptide	624:651	a hydrophobic signal peptide at the N-terminus	624:669	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	3	29	from	N-terminus	660:669	arg1	propeptide					674:683	a propeptide	672:683	a propeptide at the C-terminus	672:701	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	3	29	from	N-terminus	660:669	arg1	residue					731:737	a single cysteine (Cys) residue	707:737	a single cysteine (Cys) residue for dimer formation	707:757	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	6	30	with	WFA	1041:1043	arg1	substitution					1064:1075	an amino acid substitution	1050:1075	an amino acid substitution	1050:1075	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	3	31	theme	nucleotides	571:581	arg1	frame					558:562	an open reading frame	542:562	an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation	542:757	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	4	32	dep	microarray	820:829	arg1	the					801:803	the	801:803	the	801:803	We characterized the natural and rWFA by the glycoconjugate microarray and frontal affinity chromatography.
28549117	3	33	theme	cysteine	716:723	arg1	residue					731:737	a single cysteine (Cys) residue	707:737	a single cysteine (Cys) residue for dimer formation	707:757	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	6	34	theme	acid	1059:1062	arg1	substitution					1064:1075	an amino acid substitution	1050:1075	an amino acid substitution	1050:1075	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	6	35	with	lectin	1120:1125	arg1	affinity					1174:1181	wild-type affinity	1164:1181	wild-type affinity for GalNAc-terminated glycans	1164:1211	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	6	35	with	lectin	1120:1125	arg1	GalNAcβ1,4GlcNAc					1227:1242	GalNAcβ1,4GlcNAc	1227:1242	GalNAcβ1,4GlcNAc	1227:1242	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	6	35	with	lectin	1120:1125	arg1	specificity					1148:1158	limited binding specificity	1132:1158	limited binding specificity	1132:1158	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	4	36	theme	affinity	843:850	arg1	chromatography					852:865	frontal affinity chromatography	835:865	frontal affinity chromatography	835:865	We characterized the natural and rWFA by the glycoconjugate microarray and frontal affinity chromatography.
28549117	5	37	theme	-terminated	995:1005	arg1	glycans					1007:1013	Gal- and N-acetylgalactosamine (GalNAc)-terminated glycans	956:1013	Gal- and N-acetylgalactosamine (GalNAc)-terminated glycans	956:1013	rWFA exhibited glycan binding specificity similar to that of natural WFA: both bound to Gal- and N-acetylgalactosamine (GalNAc)-terminated glycans.
28549117	6	38	theme	GalNAc-terminated	1187:1203	arg1	glycans					1205:1211	GalNAc-terminated glycans	1187:1211	GalNAc-terminated glycans	1187:1211	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	1	39	from	alterations	203:213	arg1	diseases					218:225	diseases	218:225	diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis	218:287	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	1	39	from	alterations	203:213	arg1	carcinoma					258:266	intrahepatic bile duct carcinoma	235:266	intrahepatic bile duct carcinoma	235:266	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	1	39	from	alterations	203:213	arg1	fibrosis					280:287	hepatic fibrosis	272:287	hepatic fibrosis	272:287	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	5	40	theme	glycan	883:888	arg1	specificity					898:908	glycan binding specificity	883:908	glycan binding specificity similar to that of natural WFA	883:939	rWFA exhibited glycan binding specificity similar to that of natural WFA: both bound to Gal- and N-acetylgalactosamine (GalNAc)-terminated glycans.
28549117	3	41	theme	Cys	726:728	arg1	residue					731:737	a single cysteine (Cys) residue	707:737	a single cysteine (Cys) residue for dimer formation	707:757	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	3	42	theme	WFA	594:596	arg1	precursor					598:606	a WFA precursor	592:606	a WFA precursor	592:606	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	5	43	theme	binding	890:896	arg1	specificity					898:908	glycan binding specificity	883:908	glycan binding specificity similar to that of natural WFA	883:939	rWFA exhibited glycan binding specificity similar to that of natural WFA: both bound to Gal- and N-acetylgalactosamine (GalNAc)-terminated glycans.
28549117	6	44	theme	recombinant	1098:1108	arg1	lectin					1120:1125	a recombinant monomeric lectin	1096:1125	a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc	1096:1242	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	3	45	theme	hydrophobic	626:636	arg1	peptide					645:651	a hydrophobic signal peptide	624:651	a hydrophobic signal peptide at the N-terminus	624:669	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	5	46	theme	natural	929:935	arg1	WFA					937:939	natural WFA	929:939	natural WFA	929:939	rWFA exhibited glycan binding specificity similar to that of natural WFA: both bound to Gal- and N-acetylgalactosamine (GalNAc)-terminated glycans.
28549117	7	47	theme	engineered	1256:1265	arg1	lectin					1267:1272	this engineered lectin	1251:1272	this engineered lectin	1251:1272	Thus, this engineered lectin may be applied to highly sensitive biomarker detection.
28549117	1	48	theme	intrahepatic	235:246	arg1	carcinoma					258:266	intrahepatic bile duct carcinoma	235:266	intrahepatic bile duct carcinoma	235:266	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	3	49	theme	signal	638:643	arg1	peptide					645:651	a hydrophobic signal peptide	624:651	a hydrophobic signal peptide at the N-terminus	624:669	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	6	50	theme	wild-type	1164:1172	arg1	affinity					1174:1181	wild-type affinity	1164:1181	wild-type affinity for GalNAc-terminated glycans	1164:1211	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	3	51	theme	DNA	490:492	arg1	sequence					494:501	The natural complementary DNA sequence	464:501	The natural complementary DNA sequence obtained from wisteria seeds	464:530	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	1	52	theme	bile	248:251	arg1	carcinoma					258:266	intrahepatic bile duct carcinoma	235:266	intrahepatic bile duct carcinoma	235:266	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
28549117	5	53	dep	-terminated	995:1005	arg1	Gal-					956:959	Gal-	956:959	Gal-	956:959	rWFA exhibited glycan binding specificity similar to that of natural WFA: both bound to Gal- and N-acetylgalactosamine (GalNAc)-terminated glycans.
28549117	5	53	dep	-terminated	995:1005	arg1	N-acetylgalactosamine					965:985	N-acetylgalactosamine	965:985	N-acetylgalactosamine	965:985	rWFA exhibited glycan binding specificity similar to that of natural WFA: both bound to Gal- and N-acetylgalactosamine (GalNAc)-terminated glycans.
28549117	3	54	theme	dimer	743:747	arg1	formation					749:757	dimer formation	743:757	dimer formation	743:757	The natural complementary DNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation.
28549117	6	55	theme	amino	1053:1057	arg1	substitution					1064:1075	an amino acid substitution	1050:1075	an amino acid substitution	1050:1075	Moreover, the engineered WFA with an amino acid substitution in Cys-272 yielded a recombinant monomeric lectin with limited binding specificity but wild-type affinity for GalNAc-terminated glycans, specifically GalNAcβ1,4GlcNAc.
28549117	1	56	theme	duct	253:256	arg1	carcinoma					258:266	intrahepatic bile duct carcinoma	235:266	intrahepatic bile duct carcinoma	235:266	Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified.
27209382	2	0	theme	emulsions	320:328	arg1	stability					293:301	stability	293:301	stability	293:301	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	2	0	theme	emulsions	320:328	arg1	properties					278:287	properties	278:287	properties	278:287	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	5	1	from	interface	1028:1036	arg1	adsorption					989:998	multi-layered adsorption	975:998	multi-layered adsorption of pectins at the oil-water interface	975:1036	Examination of chemical composition of interfacial layers indicated multi-layered adsorption of pectins at the oil-water interface.
27209382	1	2	theme	n-alkane-in-water	223:239	arg1	emulsions					241:249	model n-alkane-in-water emulsions	217:249	model n-alkane-in-water emulsions	217:249	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	5	3	theme	chemical	922:929	arg1	composition					931:941	chemical composition	922:941	chemical composition of interfacial layers	922:963	Examination of chemical composition of interfacial layers indicated multi-layered adsorption of pectins at the oil-water interface.
27209382	2	4	theme	resulting	310:318	arg1	emulsions					320:328	the resulting emulsions	306:328	the resulting emulsions	306:328	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	1	5	theme	emulsions	241:249	arg1	stabilisation					191:203	stabilisation	191:203	stabilisation	191:203	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	1	5	theme	emulsions	241:249	arg1	formation					177:185	formation	177:185	formation	177:185	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	6	6	theme	low	1210:1212	arg1	volume					1227:1232	the low hydrodynamic volume	1206:1232	the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability	1206:1372	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	6	6	theme	low	1210:1212	arg1	interfaces					1163:1172	mechanically stronger interfaces	1141:1172	mechanically stronger interfaces	1141:1172	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	4	7	theme	droplet	789:795	arg1	growth					797:802	droplet growth	789:802	droplet growth	789:802	Emulsions prepared using pectin isolated at pH 6.0 were remarkably stable with respect to droplet growth after thirty days of ageing, while those prepared with pectin isolated at pH 2.0 destabilised rapidly.
27209382	1	8	theme	acidic	254:259	arg1	pH					265:266	pH 2.0	265:270	pH 2.0	265:270	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	1	8	theme	acidic	254:259	arg1	pH					261:262	acidic pH	254:262	acidic pH (pH 2.0)	254:271	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	2	9	theme	dilatational	512:523	arg1	rheology					525:532	large-amplitude oscillatory surface dilatational rheology	476:532	large-amplitude oscillatory surface dilatational rheology	476:532	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	6	10	theme	branched	1186:1193	arg1	nature					1195:1200	the highly branched nature	1175:1200	the highly branched nature	1175:1200	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	6	10	theme	branched	1186:1193	arg1	interfaces					1163:1172	mechanically stronger interfaces	1141:1172	mechanically stronger interfaces	1141:1172	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	1	11	theme	distinct	62:69	arg1	design					81:86	distinct molecular design	62:86	distinct molecular design	62:86	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	3	12	from	alkanes	635:641	arg1	water					646:650	water	646:650	water	646:650	Both pectin preparations were able to emulsify alkanes in water but exhibited distinct ageing characteristics.
27209382	2	13	theme	droplet	356:362	arg1	analysis					382:389	droplet size distribution analysis	356:389	droplet size distribution analysis	356:389	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	2	14	theme	surface	504:510	arg1	rheology					525:532	large-amplitude oscillatory surface dilatational rheology	476:532	large-amplitude oscillatory surface dilatational rheology	476:532	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	5	15	theme	composition	931:941	arg1	Examination					907:917	Examination	907:917	Examination of chemical composition of interfacial layers	907:963	Examination of chemical composition of interfacial layers indicated multi-layered adsorption of pectins at the oil-water interface.
27209382	6	16	theme	methyl	1313:1318	arg1	contents					1320:1327	methyl contents	1313:1327	methyl contents	1313:1327	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	1	17	theme	molecular	71:79	arg1	design					81:86	distinct molecular design	62:86	distinct molecular design	62:86	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	7	18	theme	pectin	1453:1458	arg1	structure					1440:1448	the fine structure	1431:1448	the fine structure of pectin	1431:1458	The present work shows that it is possible by tailoring the fine structure of pectin to engineer emulsions that operate in acidic environments.
27209382	2	19	theme	bulk	427:430	arg1	rheology					432:439	bulk rheology	427:439	bulk rheology	427:439	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	7	20	theme	acidic	1498:1503	arg1	environments					1505:1516	acidic environments	1498:1516	acidic environments	1498:1516	The present work shows that it is possible by tailoring the fine structure of pectin to engineer emulsions that operate in acidic environments.
27209382	5	21	theme	multi-layered	975:987	arg1	adsorption					989:998	multi-layered adsorption	975:998	multi-layered adsorption of pectins at the oil-water interface	975:1036	Examination of chemical composition of interfacial layers indicated multi-layered adsorption of pectins at the oil-water interface.
27209382	6	22	theme	emulsions	1073:1081	arg1	stability					1060:1068	The higher long-term stability	1039:1068	The higher long-term stability of emulsions prepared with pectin isolated at high pH	1039:1122	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	3	23	theme	pectin	593:598	arg1	preparations					600:611	Both pectin preparations	588:611	Both pectin preparations	588:611	Both pectin preparations were able to emulsify alkanes in water but exhibited distinct ageing characteristics.
27209382	6	24	theme	long-term	1050:1058	arg1	stability					1060:1068	The higher long-term stability	1039:1068	The higher long-term stability of emulsions prepared with pectin isolated at high pH	1039:1122	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	3	25	theme	ageing	675:680	arg1	characteristics					682:696	distinct ageing characteristics	666:696	distinct ageing characteristics	666:696	Both pectin preparations were able to emulsify alkanes in water but exhibited distinct ageing characteristics.
27209382	0	26	theme	O/W	22:24	arg1	emulsions					26:34	acidic O/W emulsions	15:34	acidic O/W emulsions	15:34	Engineering of acidic O/W emulsions with pectin.
27209382	2	27	theme	fluorescence	563:574	arg1	microscopy					576:585	fluorescence microscopy	563:585	fluorescence microscopy	563:585	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	0	28	theme	acidic	15:20	arg1	emulsions					26:34	acidic O/W emulsions	15:34	acidic O/W emulsions	15:34	Engineering of acidic O/W emulsions with pectin.
27209382	5	29	theme	oil-water	1018:1026	arg1	interface					1028:1036	the oil-water interface	1014:1036	the oil-water interface	1014:1036	Examination of chemical composition of interfacial layers indicated multi-layered adsorption of pectins at the oil-water interface.
27209382	3	30	theme	distinct	666:673	arg1	characteristics					682:696	distinct ageing characteristics	666:696	distinct ageing characteristics	666:696	Both pectin preparations were able to emulsify alkanes in water but exhibited distinct ageing characteristics.
27209382	5	31	theme	pectins	1003:1009	arg1	adsorption					989:998	multi-layered adsorption	975:998	multi-layered adsorption of pectins at the oil-water interface	975:1036	Examination of chemical composition of interfacial layers indicated multi-layered adsorption of pectins at the oil-water interface.
27209382	2	32	theme	composition	454:464	arg1	analysis					466:473	interfacial composition analysis	442:473	interfacial composition analysis	442:473	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	1	33	theme	formation	177:185	arg1	terms					162:166	terms	162:166	terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions	162:249	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	6	34	theme	chains	1241:1246	arg1	volume					1227:1232	the low hydrodynamic volume	1206:1232	the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability	1206:1372	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	6	34	theme	chains	1241:1246	arg1	nature					1195:1200	the highly branched nature	1175:1200	the highly branched nature	1175:1200	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	6	34	theme	chains	1241:1246	arg1	interfaces					1163:1172	mechanically stronger interfaces	1141:1172	mechanically stronger interfaces	1141:1172	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	2	35	theme	distribution	369:380	arg1	analysis					382:389	droplet size distribution analysis	356:389	droplet size distribution analysis	356:389	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	0	36	theme	emulsions	26:34	arg1	Engineering					0:10	Engineering	0:10	Engineering of acidic O/W emulsions with pectin.	0:47	Engineering of acidic O/W emulsions with pectin.
27209382	2	37	theme	interfacial	442:452	arg1	analysis					466:473	interfacial composition analysis	442:473	interfacial composition analysis	442:473	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	6	38	theme	higher	1043:1048	arg1	stability					1060:1068	The higher long-term stability	1039:1068	The higher long-term stability of emulsions prepared with pectin isolated at high pH	1039:1122	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	7	39	theme	fine	1435:1438	arg1	structure					1440:1448	the fine structure	1431:1448	the fine structure of pectin	1431:1458	The present work shows that it is possible by tailoring the fine structure of pectin to engineer emulsions that operate in acidic environments.
27209382	2	40	theme	size	364:367	arg1	analysis					382:389	droplet size distribution analysis	356:389	droplet size distribution analysis	356:389	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	1	41	with	Pectins	49:55	arg1	design					81:86	distinct molecular design	62:86	distinct molecular design	62:86	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	6	42	theme	steric	1273:1278	arg1	stabilisation					1280:1292	effective steric stabilisation	1263:1292	effective steric stabilisation	1263:1292	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	1	43	theme	aqueous	105:111	arg1	extraction					113:122	aqueous extraction	105:122	aqueous extraction	105:122	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	2	44	theme	electrokinetic	535:548	arg1	analysis					550:557	electrokinetic analysis	535:557	electrokinetic analysis	535:557	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	7	45	theme	present	1379:1385	arg1	work					1387:1390	The present work	1375:1390	The present work	1375:1390	The present work shows that it is possible by tailoring the fine structure of pectin to engineer emulsions that operate in acidic environments.
27209382	6	46	theme	effective	1263:1271	arg1	stabilisation					1280:1292	effective steric stabilisation	1263:1292	effective steric stabilisation	1263:1292	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	5	47	theme	interfacial	946:956	arg1	layers					958:963	interfacial layers	946:963	interfacial layers	946:963	Examination of chemical composition of interfacial layers indicated multi-layered adsorption of pectins at the oil-water interface.
27209382	4	48	theme	ageing	825:830	arg1	days					817:820	thirty days	810:820	thirty days of ageing	810:830	Emulsions prepared using pectin isolated at pH 6.0 were remarkably stable with respect to droplet growth after thirty days of ageing, while those prepared with pectin isolated at pH 2.0 destabilised rapidly.
27209382	6	49	theme	long-term	1354:1362	arg1	stability					1364:1372	the long-term stability	1350:1372	the long-term stability	1350:1372	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	1	50	dep	formation	177:185	arg1	capacity					205:212	capacity	205:212	capacity	205:212	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	0	51	with	Engineering	0:10	arg1	pectin					41:46	pectin	41:46	pectin	41:46	Engineering of acidic O/W emulsions with pectin.
27209382	2	52	dep	properties	278:287	arg1	The					274:276	The	274:276	The	274:276	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	1	53	theme	stabilisation	191:203	arg1	terms					162:166	terms	162:166	terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions	162:249	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
27209382	6	54	theme	hydrodynamic	1214:1225	arg1	volume					1227:1232	the low hydrodynamic volume	1206:1232	the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability	1206:1372	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	6	54	theme	hydrodynamic	1214:1225	arg1	interfaces					1163:1172	mechanically stronger interfaces	1141:1172	mechanically stronger interfaces	1141:1172	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	6	55	theme	high	1116:1119	arg1	pH					1121:1122	high pH	1116:1122	high pH	1116:1122	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	5	56	theme	layers	958:963	arg1	composition					931:941	chemical composition	922:941	chemical composition of interfacial layers	922:963	Examination of chemical composition of interfacial layers indicated multi-layered adsorption of pectins at the oil-water interface.
27209382	2	57	theme	oscillatory	492:502	arg1	rheology					525:532	large-amplitude oscillatory surface dilatational rheology	476:532	large-amplitude oscillatory surface dilatational rheology	476:532	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	2	58	theme	large-amplitude	476:490	arg1	rheology					525:532	large-amplitude oscillatory surface dilatational rheology	476:532	large-amplitude oscillatory surface dilatational rheology	476:532	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	2	59	theme	Lifshitz-Slyozov-Wagner	392:414	arg1	modelling					416:424	Lifshitz-Slyozov-Wagner modelling	392:424	Lifshitz-Slyozov-Wagner modelling	392:424	The properties and stability of the resulting emulsions were examined by means of droplet size distribution analysis, Lifshitz-Slyozov-Wagner modelling, bulk rheology, interfacial composition analysis, large-amplitude oscillatory surface dilatational rheology, electrokinetic analysis and fluorescence microscopy.
27209382	6	60	theme	stronger	1154:1161	arg1	volume					1227:1232	the low hydrodynamic volume	1206:1232	the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability	1206:1372	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	6	60	theme	stronger	1154:1161	arg1	nature					1195:1200	the highly branched nature	1175:1200	the highly branched nature	1175:1200	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	6	60	theme	stronger	1154:1161	arg1	interfaces					1163:1172	mechanically stronger interfaces	1141:1172	mechanically stronger interfaces	1141:1172	The higher long-term stability of emulsions prepared with pectin isolated at high pH is attributed to mechanically stronger interfaces, the highly branched nature and the low hydrodynamic volume of the chains that result in effective steric stabilisation whereas acetyl and methyl contents do not contribute to the long-term stability.
27209382	1	61	theme	model	217:221	arg1	emulsions					241:249	model n-alkane-in-water emulsions	217:249	model n-alkane-in-water emulsions	217:249	Pectins with distinct molecular design were isolated by aqueous extraction at pH 2.0 or 6.0 and were examined in terms of their formation and stabilisation capacity of model n-alkane-in-water emulsions at acidic pH (pH 2.0).
25970747	3	0	theme	hyphenated	706:715	arg1	UHPLC-DAD-MS/MS					755:769	UHPLC-DAD-MS/MS	755:769	UHPLC-DAD-MS/MS	755:769	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	0	theme	hyphenated	706:715	arg1	spectrometry					741:752	hyphenated diode array tandem mass spectrometry	706:752	hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS)	706:770	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	5	1	theme	furan-2-yl	1071:1080	arg1	methanol					1082:1089	(5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol	1042:1089	(5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol	1042:1089	Further, a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD could be characterized to be (5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol.
25970747	5	1	theme	furan-2-yl	1071:1080	arg1	product					962:968	a reaction product	951:968	a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD	951:1011	Further, a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD could be characterized to be (5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol.
25970747	1	2	theme	partial	152:158	arg1	degradation					160:170	partial degradation	152:170	partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy	152:322	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	3	3	theme	tandem	729:734	arg1	UHPLC-DAD-MS/MS					755:769	UHPLC-DAD-MS/MS	755:769	UHPLC-DAD-MS/MS	755:769	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	3	theme	tandem	729:734	arg1	spectrometry					741:752	hyphenated diode array tandem mass spectrometry	706:752	hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS)	706:770	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	0	4	from	Profiling	12:20	arg1	Fluids					81:86	Peritoneal Dialysis Fluids	61:86	Peritoneal Dialysis Fluids	61:86	Qualitative Profiling of Polyglucose Degradation Products in Peritoneal Dialysis Fluids.
25970747	3	5	from	products	503:510	arg1	pGDPs					562:566	pGDPs	562:566	pGDPs	562:566	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	5	from	products	503:510	arg1	fluids					554:559	polyglucose (icodextrin)-containing PD fluids	515:559	polyglucose (icodextrin)-containing PD fluids (pGDPs)	515:567	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	5	6	theme	5-hydroxymethylfurfural	973:995	arg1	methanol					1082:1089	(5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol	1042:1089	(5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol	1042:1089	Further, a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD could be characterized to be (5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol.
25970747	5	6	theme	5-hydroxymethylfurfural	973:995	arg1	product					962:968	a reaction product	951:968	a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD	951:1011	Further, a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD could be characterized to be (5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol.
25970747	3	7	theme	-containing	539:549	arg1	pGDPs					562:566	pGDPs	562:566	pGDPs	562:566	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	7	theme	-containing	539:549	arg1	fluids					554:559	polyglucose (icodextrin)-containing PD fluids	515:559	polyglucose (icodextrin)-containing PD fluids (pGDPs)	515:567	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	7	8	theme	imidazol-2-yl	1353:1365	arg1	-3,4-dihydroxybutan-1-one					1367:1391	1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one	1334:1391	1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one	1334:1391	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	6	9	from	fluids	1208:1213	arg1	present					1180:1186	present	1180:1186	present	1180:1186	Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids.
25970747	4	10	theme	PD	931:932	arg1	fluids					934:939	PD fluids	931:939	PD fluids	931:939	For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids.
25970747	3	11	theme	liquid	679:684	arg1	chromatography					686:699	ultrahigh-performance liquid chromatography	657:699	ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS)	657:770	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	12	theme	PD	551:552	arg1	pGDPs					562:566	pGDPs	562:566	pGDPs	562:566	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	12	theme	PD	551:552	arg1	fluids					554:559	polyglucose (icodextrin)-containing PD fluids	515:559	polyglucose (icodextrin)-containing PD fluids (pGDPs)	515:567	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	13	theme	mass	736:739	arg1	UHPLC-DAD-MS/MS					755:769	UHPLC-DAD-MS/MS	755:769	UHPLC-DAD-MS/MS	755:769	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	13	theme	mass	736:739	arg1	spectrometry					741:752	hyphenated diode array tandem mass spectrometry	706:752	hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS)	706:770	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	14	with	chromatography	686:699	arg1	UHPLC-DAD-MS/MS					755:769	UHPLC-DAD-MS/MS	755:769	UHPLC-DAD-MS/MS	755:769	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	14	with	chromatography	686:699	arg1	spectrometry					741:752	hyphenated diode array tandem mass spectrometry	706:752	hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS)	706:770	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	4	15	theme	first	781:785	arg1	time					787:790	the first time	777:790	the first time	777:790	For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids.
25970747	7	16	theme	degradation	1297:1307	arg1	product					1309:1315	a hitherto unknown degradation product	1278:1315	Trapping a hitherto unknown degradation product with OPD	1269:1324	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	7	17	from	fluids	1453:1458	arg1	present					1404:1410	present	1404:1410	present	1404:1410	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	3	18	theme	diode	717:721	arg1	UHPLC-DAD-MS/MS					755:769	UHPLC-DAD-MS/MS	755:769	UHPLC-DAD-MS/MS	755:769	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	18	theme	diode	717:721	arg1	spectrometry					741:752	hyphenated diode array tandem mass spectrometry	706:752	hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS)	706:770	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	6	19	theme	PD	1205:1206	arg1	fluids					1208:1213	glucose-based PD fluids	1191:1213	glucose-based PD fluids	1191:1213	Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids.
25970747	1	20	theme	Heat	89:92	arg1	sterilization					94:106	Heat sterilization	89:106	Heat sterilization of peritoneal dialysis (PD) fluids	89:141	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	7	21	theme	heat-	1415:1419	arg1	fluids					1453:1458	heat- as well as filter-sterilized PD fluids	1415:1458	heat- as well as filter-sterilized PD fluids	1415:1458	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	7	22	theme	unknown	1289:1295	arg1	product					1309:1315	a hitherto unknown degradation product	1278:1315	Trapping a hitherto unknown degradation product with OPD	1269:1324	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	2	23	theme	fluids	440:445	arg1	biocompatibility					417:432	biocompatibility	417:432	biocompatibility of PD fluids	417:445	Hence, it is important to know the exact composition of the degradation products to improve biocompatibility of PD fluids.
25970747	3	24	theme	array	723:727	arg1	UHPLC-DAD-MS/MS					755:769	UHPLC-DAD-MS/MS	755:769	UHPLC-DAD-MS/MS	755:769	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	24	theme	array	723:727	arg1	spectrometry					741:752	hyphenated diode array tandem mass spectrometry	706:752	hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS)	706:770	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	6	25	theme	glucose-based	1191:1203	arg1	fluids					1208:1213	glucose-based PD fluids	1191:1213	glucose-based PD fluids	1191:1213	Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids.
25970747	1	26	theme	osmotic	179:185	arg1	agent					187:191	the osmotic agent	175:191	the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy	175:322	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	0	27	theme	Qualitative	0:10	arg1	Profiling					12:20	Qualitative Profiling	0:20	Qualitative Profiling of Polyglucose Degradation Products in Peritoneal Dialysis Fluids	0:86	Qualitative Profiling of Polyglucose Degradation Products in Peritoneal Dialysis Fluids.
25970747	1	28	theme	PD	282:283	arg1	fluids					285:290	PD fluids	282:290	PD fluids	282:290	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	1	29	theme	agent	187:191	arg1	degradation					160:170	partial degradation	152:170	partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy	152:322	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	0	30	theme	Polyglucose	25:35	arg1	Products					49:56	Polyglucose Degradation Products	25:56	Polyglucose Degradation Products	25:56	Qualitative Profiling of Polyglucose Degradation Products in Peritoneal Dialysis Fluids.
25970747	1	31	theme	fluids	285:290	arg1	biocompatibility					262:277	the biocompatibility	258:277	the biocompatibility of PD fluids	258:290	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	7	32	theme	1,4-bis	1334:1340	arg1	-3,4-dihydroxybutan-1-one					1367:1391	1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one	1334:1391	1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one	1334:1391	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	1	33	theme	peritoneal	111:120	arg1	fluids					136:141	peritoneal dialysis (PD) fluids	111:141	peritoneal dialysis (PD) fluids	111:141	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	6	34	located	detected	1226:1233	arg1	fluids					1261:1266	polyglucose-containing fluids	1238:1266	polyglucose-containing fluids	1238:1266	Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids.
25970747	6	34	located	detected	1226:1233	arg2	3-DGal					1154:1159	3-DGal	1154:1159	3-DGal	1154:1159	Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids.
25970747	6	34	located	detected	1226:1233	arg2	3-deoxygalactosone					1134:1151	3-deoxygalactosone	1134:1151	3-deoxygalactosone (3-DGal)	1134:1160	Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids.
25970747	6	34	located	detected	1226:1233	arg2	3-DG					1124:1127	3-DG	1124:1127	3-DG	1124:1127	Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids.
25970747	6	34	located	detected	1226:1233	arg2	3-deoxyglucosone					1106:1121	3-deoxyglucosone	1106:1121	3-deoxyglucosone (3-DG)	1106:1128	Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids.
25970747	2	35	theme	PD	437:438	arg1	fluids					440:445	PD fluids	437:445	PD fluids	437:445	Hence, it is important to know the exact composition of the degradation products to improve biocompatibility of PD fluids.
25970747	1	36	theme	dialysis	122:129	arg1	fluids					136:141	peritoneal dialysis (PD) fluids	111:141	peritoneal dialysis (PD) fluids	111:141	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	0	37	theme	Products	49:56	arg1	Profiling					12:20	Qualitative Profiling	0:20	Qualitative Profiling of Polyglucose Degradation Products in Peritoneal Dialysis Fluids	0:86	Qualitative Profiling of Polyglucose Degradation Products in Peritoneal Dialysis Fluids.
25970747	3	38	theme	degradation	491:501	arg1	products					503:510	degradation products	491:510	degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs)	491:567	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	2	39	theme	products	397:404	arg1	composition					366:376	the exact composition	356:376	the exact composition of the degradation products to improve biocompatibility of PD fluids	356:445	Hence, it is important to know the exact composition of the degradation products to improve biocompatibility of PD fluids.
25970747	7	40	dep	Trapping	1269:1276	arg1	product					1309:1315	a hitherto unknown degradation product	1278:1315	Trapping a hitherto unknown degradation product with OPD	1269:1324	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	2	41	theme	exact	360:364	arg1	composition					366:376	the exact composition	356:376	the exact composition of the degradation products to improve biocompatibility of PD fluids	356:445	Hence, it is important to know the exact composition of the degradation products to improve biocompatibility of PD fluids.
25970747	1	42	theme	reactive	201:208	arg1	structures					219:228	reactive carbonyl structures	201:228	reactive carbonyl structures	201:228	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	0	43	theme	Degradation	37:47	arg1	Products					49:56	Polyglucose Degradation Products	25:56	Polyglucose Degradation Products	25:56	Qualitative Profiling of Polyglucose Degradation Products in Peritoneal Dialysis Fluids.
25970747	1	44	theme	long-term	303:311	arg1	therapy					316:322	long-term PD therapy	303:322	long-term PD therapy	303:322	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	3	45	theme	stable	614:619	arg1	derivatives					621:631	stable derivatives	614:631	stable derivatives	614:631	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	7	46	theme	1H-benzo[d	1342:1351	arg1	-3,4-dihydroxybutan-1-one					1367:1391	1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one	1334:1391	1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one	1334:1391	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	1	47	theme	carbonyl	210:217	arg1	structures					219:228	reactive carbonyl structures	201:228	reactive carbonyl structures	201:228	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	0	48	theme	Peritoneal	61:70	arg1	Fluids					81:86	Peritoneal Dialysis Fluids	61:86	Peritoneal Dialysis Fluids	61:86	Qualitative Profiling of Polyglucose Degradation Products in Peritoneal Dialysis Fluids.
25970747	1	49	theme	PD	313:314	arg1	therapy					316:322	long-term PD therapy	303:322	long-term PD therapy	303:322	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	7	50	with	product	1309:1315	arg1	OPD					1322:1324	OPD	1322:1324	OPD	1322:1324	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	5	51	theme	OPD	1009:1011	arg1	methanol					1082:1089	(5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol	1042:1089	(5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol	1042:1089	Further, a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD could be characterized to be (5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol.
25970747	5	51	theme	OPD	1009:1011	arg1	product					962:968	a reaction product	951:968	a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD	951:1011	Further, a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD could be characterized to be (5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol.
25970747	7	52	theme	filter-sterilized	1432:1448	arg1	fluids					1453:1458	heat- as well as filter-sterilized PD fluids	1415:1458	heat- as well as filter-sterilized PD fluids	1415:1458	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	5	53	dep	methanol	1082:1089	arg1	imidazol-2-yl					1057:1069	imidazol-2-yl	1057:1069	imidazol-2-yl	1057:1069	Further, a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD could be characterized to be (5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol.
25970747	4	54	theme	polyglucose	826:836	arg1	4-deoxyglucosone					847:862	4-deoxyglucosone	847:862	4-deoxyglucosone (4-DG)	847:869	For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids.
25970747	4	54	theme	polyglucose	826:836	arg1	products					814:821	specific degradation products	793:821	specific degradation products of polyglucose	793:836	For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids.
25970747	4	54	theme	polyglucose	826:836	arg1	3,4-dideoxypentosone					875:894	3,4-dideoxypentosone	875:894	3,4-dideoxypentosone (3,4-DDPS)	875:905	For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids.
25970747	7	55	located	present	1404:1410	arg2	-3,4-dihydroxybutan-1-one					1367:1391	1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one	1334:1391	1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one	1334:1391	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	7	55	located	present	1404:1410	arg1	fluids					1453:1458	heat- as well as filter-sterilized PD fluids	1415:1458	heat- as well as filter-sterilized PD fluids	1415:1458	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	1	56	theme	PD	132:133	arg1	fluids					136:141	peritoneal dialysis (PD) fluids	111:141	peritoneal dialysis (PD) fluids	111:141	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	6	57	theme	polyglucose-containing	1238:1259	arg1	fluids					1261:1266	polyglucose-containing fluids	1238:1266	polyglucose-containing fluids	1238:1266	Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids.
25970747	3	58	theme	ultrahigh-performance	657:677	arg1	chromatography					686:699	ultrahigh-performance liquid chromatography	657:699	ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS)	657:770	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	3	59	theme	targeted	468:475	arg1	screening					477:485	targeted screening	468:485	targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs)	468:567	Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC-DAD-MS/MS).
25970747	7	60	from	present	1404:1410	arg1	fluids					1453:1458	heat- as well as filter-sterilized PD fluids	1415:1458	heat- as well as filter-sterilized PD fluids	1415:1458	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	5	61	theme	reaction	953:960	arg1	methanol					1082:1089	(5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol	1042:1089	(5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol	1042:1089	Further, a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD could be characterized to be (5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol.
25970747	5	61	theme	reaction	953:960	arg1	product					962:968	a reaction product	951:968	a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD	951:1011	Further, a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD could be characterized to be (5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol.
25970747	1	62	theme	fluids	136:141	arg1	sterilization					94:106	Heat sterilization	89:106	Heat sterilization of peritoneal dialysis (PD) fluids	89:141	Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy.
25970747	6	63	from	present	1180:1186	arg1	fluids					1208:1213	glucose-based PD fluids	1191:1213	glucose-based PD fluids	1191:1213	Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids.
25970747	2	64	theme	degradation	385:395	arg1	products					397:404	the degradation products	381:404	the degradation products	381:404	Hence, it is important to know the exact composition of the degradation products to improve biocompatibility of PD fluids.
25970747	4	65	theme	degradation	802:812	arg1	4-deoxyglucosone					847:862	4-deoxyglucosone	847:862	4-deoxyglucosone (4-DG)	847:869	For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids.
25970747	4	65	theme	degradation	802:812	arg1	products					814:821	specific degradation products	793:821	specific degradation products of polyglucose	793:836	For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids.
25970747	4	65	theme	degradation	802:812	arg1	3,4-dideoxypentosone					875:894	3,4-dideoxypentosone	875:894	3,4-dideoxypentosone (3,4-DDPS)	875:905	For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids.
25970747	0	66	theme	Dialysis	72:79	arg1	Fluids					81:86	Peritoneal Dialysis Fluids	61:86	Peritoneal Dialysis Fluids	61:86	Qualitative Profiling of Polyglucose Degradation Products in Peritoneal Dialysis Fluids.
25970747	7	67	theme	PD	1450:1451	arg1	fluids					1453:1458	heat- as well as filter-sterilized PD fluids	1415:1458	heat- as well as filter-sterilized PD fluids	1415:1458	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	4	68	theme	specific	793:800	arg1	4-deoxyglucosone					847:862	4-deoxyglucosone	847:862	4-deoxyglucosone (4-DG)	847:869	For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids.
25970747	4	68	theme	specific	793:800	arg1	products					814:821	specific degradation products	793:821	specific degradation products of polyglucose	793:836	For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids.
25970747	4	68	theme	specific	793:800	arg1	3,4-dideoxypentosone					875:894	3,4-dideoxypentosone	875:894	3,4-dideoxypentosone (3,4-DDPS)	875:905	For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids.
25970747	7	69	attach	present	1404:1410	arg2	-3,4-dihydroxybutan-1-one					1367:1391	1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one	1334:1391	1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one	1334:1391	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25970747	7	69	attach	present	1404:1410	arg1	fluids					1453:1458	heat- as well as filter-sterilized PD fluids	1415:1458	heat- as well as filter-sterilized PD fluids	1415:1458	Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.
25110918	6	0	theme	brominated	918:927	arg1	compounds					944:952	the brominated skeleton-bound compounds	914:952	the brominated skeleton-bound compounds	914:952	The investigations of the skeletons before and after MeOH extraction confirmed that only a small amount of the brominated skeleton-bound compounds dissolves in MeOH.
25110918	0	1	theme	cavernicola	70:80	arg1	composition					24:34	The skeletal amino acid composition	0:34	The skeletal amino acid composition of the marine demosponge Aplysina cavernicola	0:80	The skeletal amino acid composition of the marine demosponge Aplysina cavernicola.
25110918	7	2	theme	main	977:980	arg1	part					982:985	The main part	973:985	The main part of the brominated compounds	973:1013	The main part of the brominated compounds is strongly attached to the skeletons but can be extracted for example by using Ba(OH)2.
25110918	6	3	theme	MeOH	860:863	arg1	extraction					865:874	MeOH extraction	860:874	MeOH extraction	860:874	The investigations of the skeletons before and after MeOH extraction confirmed that only a small amount of the brominated skeleton-bound compounds dissolves in MeOH.
25110918	2	4	theme	brominated	287:296	arg1	derivatives					307:317	bioactive brominated tyrosine derivatives	277:317	bioactive brominated tyrosine derivatives	277:317	Verongida sponges are well known to produce bioactive brominated tyrosine derivatives.
25110918	7	5	attach	attached	1027:1034	arg2	part					982:985	The main part	973:985	The main part of the brominated compounds	973:1013	The main part of the brominated compounds is strongly attached to the skeletons but can be extracted for example by using Ba(OH)2.
25110918	7	5	attach	attached	1027:1034	arg1	skeletons					1043:1051	the skeletons	1039:1051	the skeletons	1039:1051	The main part of the brominated compounds is strongly attached to the skeletons but can be extracted for example by using Ba(OH)2.
25110918	2	6	theme	bioactive	277:285	arg1	derivatives					307:317	bioactive brominated tyrosine derivatives	277:317	bioactive brominated tyrosine derivatives	277:317	Verongida sponges are well known to produce bioactive brominated tyrosine derivatives.
25110918	8	7	theme	Various	1104:1110	arg1	derivatives					1133:1143	Various halogenated tyrosine derivatives	1104:1143	Various halogenated tyrosine derivatives	1104:1143	Various halogenated tyrosine derivatives were identified by GC-MS and LC-MS in these Ba(OH)2 extracts of the skeletons.
25110918	3	8	theme	brominated	355:364	arg1	compounds					366:374	brominated compounds	355:374	brominated compounds	355:374	We could recently demonstrate that brominated compounds do not exclusively occur in the cellular matrix but also in the skeletons of the marine sponges Aplysina cavernicola and Ianthella basta.
25110918	6	9	theme	small	898:902	arg1	compounds					944:952	the brominated skeleton-bound compounds	914:952	the brominated skeleton-bound compounds	914:952	The investigations of the skeletons before and after MeOH extraction confirmed that only a small amount of the brominated skeleton-bound compounds dissolves in MeOH.
25110918	6	9	theme	small	898:902	arg1	amount					904:909	only a small amount	891:909	only a small amount of the brominated skeleton-bound compounds	891:952	The investigations of the skeletons before and after MeOH extraction confirmed that only a small amount of the brominated skeleton-bound compounds dissolves in MeOH.
25110918	8	10	theme	skeletons	1213:1221	arg1	extracts					1197:1204	these Ba(OH)2 extracts	1183:1204	these Ba(OH)2 extracts of the skeletons	1183:1221	Various halogenated tyrosine derivatives were identified by GC-MS and LC-MS in these Ba(OH)2 extracts of the skeletons.
25110918	1	11	theme	order	156:160	arg1	Verongida					162:170	the order Verongida	152:170	the order Verongida	152:170	It has been discovered during the past few years that demosponges of the order Verongida such as Aplysina cavernicola exhibit chitin-based skeletons.
25110918	0	12	theme	skeletal	4:11	arg1	composition					24:34	The skeletal amino acid composition	0:34	The skeletal amino acid composition of the marine demosponge Aplysina cavernicola	0:80	The skeletal amino acid composition of the marine demosponge Aplysina cavernicola.
25110918	8	13	theme	tyrosine	1124:1131	arg1	derivatives					1133:1143	Various halogenated tyrosine derivatives	1104:1143	Various halogenated tyrosine derivatives	1104:1143	Various halogenated tyrosine derivatives were identified by GC-MS and LC-MS in these Ba(OH)2 extracts of the skeletons.
25110918	5	14	theme	present	642:648	arg1	work					650:653	the present work	638:653	the present work	638:653	In the present work, we determined the skeletal amino acid composition of the demosponge A. cavernicola especially with respect to the presence of halogenated amino acids.
25110918	8	15	theme	halogenated	1112:1122	arg1	derivatives					1133:1143	Various halogenated tyrosine derivatives	1104:1143	Various halogenated tyrosine derivatives	1104:1143	Various halogenated tyrosine derivatives were identified by GC-MS and LC-MS in these Ba(OH)2 extracts of the skeletons.
25110918	1	16	theme	Verongida	162:170	arg1	cavernicola					189:199	Aplysina cavernicola	180:199	Aplysina cavernicola	180:199	It has been discovered during the past few years that demosponges of the order Verongida such as Aplysina cavernicola exhibit chitin-based skeletons.
25110918	1	16	theme	Verongida	162:170	arg1	demosponges					137:147	demosponges	137:147	demosponges of the order Verongida such as Aplysina cavernicola	137:199	It has been discovered during the past few years that demosponges of the order Verongida such as Aplysina cavernicola exhibit chitin-based skeletons.
25110918	0	17	theme	acid	19:22	arg1	composition					24:34	The skeletal amino acid composition	0:34	The skeletal amino acid composition of the marine demosponge Aplysina cavernicola	0:80	The skeletal amino acid composition of the marine demosponge Aplysina cavernicola.
25110918	5	18	theme	acids	800:804	arg1	presence					770:777	the presence	766:777	the presence of halogenated amino acids	766:804	In the present work, we determined the skeletal amino acid composition of the demosponge A. cavernicola especially with respect to the presence of halogenated amino acids.
25110918	3	19	theme	sponges	464:470	arg1	skeletons					440:448	the skeletons	436:448	the skeletons of the marine sponges	436:470	We could recently demonstrate that brominated compounds do not exclusively occur in the cellular matrix but also in the skeletons of the marine sponges Aplysina cavernicola and Ianthella basta.
25110918	0	20	theme	amino	13:17	arg1	composition					24:34	The skeletal amino acid composition	0:34	The skeletal amino acid composition of the marine demosponge Aplysina cavernicola	0:80	The skeletal amino acid composition of the marine demosponge Aplysina cavernicola.
25110918	2	21	theme	tyrosine	298:305	arg1	derivatives					307:317	bioactive brominated tyrosine derivatives	277:317	bioactive brominated tyrosine derivatives	277:317	Verongida sponges are well known to produce bioactive brominated tyrosine derivatives.
25110918	1	22	theme	Aplysina	180:187	arg1	cavernicola					189:199	Aplysina cavernicola	180:199	Aplysina cavernicola	180:199	It has been discovered during the past few years that demosponges of the order Verongida such as Aplysina cavernicola exhibit chitin-based skeletons.
25110918	5	23	theme	halogenated	782:792	arg1	acids					800:804	halogenated amino acids	782:804	halogenated amino acids	782:804	In the present work, we determined the skeletal amino acid composition of the demosponge A. cavernicola especially with respect to the presence of halogenated amino acids.
25110918	5	24	theme	demosponge	713:722	arg1	composition					694:704	the skeletal amino acid composition	670:704	the skeletal amino acid composition of the demosponge	670:722	In the present work, we determined the skeletal amino acid composition of the demosponge A. cavernicola especially with respect to the presence of halogenated amino acids.
25110918	2	25	theme	Verongida	233:241	arg1	sponges					243:249	Verongida sponges	233:249	Verongida sponges	233:249	Verongida sponges are well known to produce bioactive brominated tyrosine derivatives.
25110918	5	26	theme	amino	794:798	arg1	acids					800:804	halogenated amino acids	782:804	halogenated amino acids	782:804	In the present work, we determined the skeletal amino acid composition of the demosponge A. cavernicola especially with respect to the presence of halogenated amino acids.
25110918	8	27	theme	Ba	1189:1190	arg1	extracts					1197:1204	these Ba(OH)2 extracts	1183:1204	these Ba(OH)2 extracts of the skeletons	1183:1221	Various halogenated tyrosine derivatives were identified by GC-MS and LC-MS in these Ba(OH)2 extracts of the skeletons.
25110918	3	28	theme	marine	457:462	arg1	sponges					464:470	the marine sponges	453:470	the marine sponges	453:470	We could recently demonstrate that brominated compounds do not exclusively occur in the cellular matrix but also in the skeletons of the marine sponges Aplysina cavernicola and Ianthella basta.
25110918	3	29	theme	cellular	408:415	arg1	matrix					417:422	the cellular matrix	404:422	the cellular matrix	404:422	We could recently demonstrate that brominated compounds do not exclusively occur in the cellular matrix but also in the skeletons of the marine sponges Aplysina cavernicola and Ianthella basta.
25110918	5	30	theme	skeletal	674:681	arg1	composition					694:704	the skeletal amino acid composition	670:704	the skeletal amino acid composition of the demosponge	670:722	In the present work, we determined the skeletal amino acid composition of the demosponge A. cavernicola especially with respect to the presence of halogenated amino acids.
25110918	0	31	theme	demosponge	50:59	arg1	cavernicola					70:80	the marine demosponge Aplysina cavernicola	39:80	the marine demosponge Aplysina cavernicola	39:80	The skeletal amino acid composition of the marine demosponge Aplysina cavernicola.
25110918	4	32	theme	unknown	552:558	arg1	compounds					560:568	these yet unknown compounds	542:568	these yet unknown compounds	542:568	Our measurements imply that these yet unknown compounds are strongly, possibly covalently bound to the sponge skeletons.
25110918	5	33	theme	amino	683:687	arg1	composition					694:704	the skeletal amino acid composition	670:704	the skeletal amino acid composition of the demosponge	670:722	In the present work, we determined the skeletal amino acid composition of the demosponge A. cavernicola especially with respect to the presence of halogenated amino acids.
25110918	6	34	theme	compounds	944:952	arg1	compounds					944:952	the brominated skeleton-bound compounds	914:952	the brominated skeleton-bound compounds	914:952	The investigations of the skeletons before and after MeOH extraction confirmed that only a small amount of the brominated skeleton-bound compounds dissolves in MeOH.
25110918	6	34	theme	compounds	944:952	arg1	amount					904:909	only a small amount	891:909	only a small amount of the brominated skeleton-bound compounds	891:952	The investigations of the skeletons before and after MeOH extraction confirmed that only a small amount of the brominated skeleton-bound compounds dissolves in MeOH.
25110918	5	35	theme	acid	689:692	arg1	composition					694:704	the skeletal amino acid composition	670:704	the skeletal amino acid composition of the demosponge	670:722	In the present work, we determined the skeletal amino acid composition of the demosponge A. cavernicola especially with respect to the presence of halogenated amino acids.
25110918	1	36	theme	past	117:120	arg1	years					126:130	the past few years	113:130	the past few years	113:130	It has been discovered during the past few years that demosponges of the order Verongida such as Aplysina cavernicola exhibit chitin-based skeletons.
25110918	4	37	theme	sponge	617:622	arg1	skeletons					624:632	the sponge skeletons	613:632	the sponge skeletons	613:632	Our measurements imply that these yet unknown compounds are strongly, possibly covalently bound to the sponge skeletons.
25110918	7	38	theme	brominated	994:1003	arg1	compounds					1005:1013	the brominated compounds	990:1013	the brominated compounds	990:1013	The main part of the brominated compounds is strongly attached to the skeletons but can be extracted for example by using Ba(OH)2.
25110918	1	39	theme	few	122:124	arg1	years					126:130	the past few years	113:130	the past few years	113:130	It has been discovered during the past few years that demosponges of the order Verongida such as Aplysina cavernicola exhibit chitin-based skeletons.
25110918	0	40	theme	Aplysina	61:68	arg1	cavernicola					70:80	the marine demosponge Aplysina cavernicola	39:80	the marine demosponge Aplysina cavernicola	39:80	The skeletal amino acid composition of the marine demosponge Aplysina cavernicola.
25110918	6	41	theme	skeletons	833:841	arg1	investigations					811:824	The investigations	807:824	The investigations of the skeletons before and after MeOH extraction	807:874	The investigations of the skeletons before and after MeOH extraction confirmed that only a small amount of the brominated skeleton-bound compounds dissolves in MeOH.
25110918	7	42	theme	compounds	1005:1013	arg1	part					982:985	The main part	973:985	The main part of the brominated compounds	973:1013	The main part of the brominated compounds is strongly attached to the skeletons but can be extracted for example by using Ba(OH)2.
25110918	0	43	theme	marine	43:48	arg1	cavernicola					70:80	the marine demosponge Aplysina cavernicola	39:80	the marine demosponge Aplysina cavernicola	39:80	The skeletal amino acid composition of the marine demosponge Aplysina cavernicola.
25110918	6	44	theme	skeleton-bound	929:942	arg1	compounds					944:952	the brominated skeleton-bound compounds	914:952	the brominated skeleton-bound compounds	914:952	The investigations of the skeletons before and after MeOH extraction confirmed that only a small amount of the brominated skeleton-bound compounds dissolves in MeOH.
25110918	1	45	theme	chitin-based	209:220	arg1	skeletons					222:230	chitin-based skeletons	209:230	chitin-based skeletons	209:230	It has been discovered during the past few years that demosponges of the order Verongida such as Aplysina cavernicola exhibit chitin-based skeletons.
26367868	2	0	theme	C	430:430	arg1	composition					409:419	composition	409:419	composition of N and C compounds	409:440	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	8	1	from	comparison	1059:1068	arg1	subtle					1096:1101	subtle	1096:1101	subtle	1096:1101	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	7	2	theme	preferred	996:1004	arg1	glycine					1006:1012	preferred glycine	996:1012	preferred glycine	996:1012	Specifically, N fungi preferred glycine over chitosan, but control fungi did not.
26367868	8	3	theme	litter	1154:1159	arg1	origin					1161:1166	litter origin	1154:1166	litter origin	1154:1166	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	6	4	theme	litter	880:885	arg1	origins					887:893	microbial and litter origins	866:893	microbial and litter origins	866:893	We found that microbial and litter origins each contributed to a shift in fungal uptake capacities under N fertilization.
26367868	5	5	theme	organic	806:812	arg1	Glycine					775:781	Glycine	775:781	Glycine	775:781	Glycine is a relatively simple organic N compound; chitosan is more complex.
26367868	5	5	theme	organic	806:812	arg1	compound					816:823	a relatively simple organic N compound	786:823	a relatively simple organic N compound	786:823	Glycine is a relatively simple organic N compound; chitosan is more complex.
26367868	2	6	from	changes	398:404	arg1	composition					409:419	composition	409:419	composition of N and C compounds	409:440	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	4	7	theme	glycine	753:759	arg1	uptake					743:748	fungal uptake	736:748	fungal uptake of glycine and chitosan	736:772	We used quantum dots (QDs) to track fungal uptake of glycine and chitosan.
26367868	2	8	from	changes	297:303	arg1	composition					350:360	community composition	340:360	community composition	340:360	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	2	8	from	changes	297:303	arg1	physiology					325:334	physiology	325:334	physiology	325:334	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	2	8	from	changes	297:303	arg1	regulation					313:322	gene regulation	308:322	gene regulation	308:322	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	9	9	theme	control	1251:1257	arg1	fungi					1259:1263	control fungi	1251:1263	control fungi	1251:1263	In particular, control fungi tended to target chitosan only when incubated with control litter, while N fungi targeted glycine regardless of litter type.
26367868	0	10	theme	Nitrogen	84:91	arg1	Enrichment					93:102	Long-Term Nitrogen Enrichment	74:102	Long-Term Nitrogen Enrichment	74:102	Quantum Dots Reveal Shifts in Organic Nitrogen Uptake by Fungi Exposed to Long-Term Nitrogen Enrichment.
26367868	2	11	dep	N	424:424	arg1	compounds					432:440	compounds	432:440	compounds	432:440	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	3	12	theme	plant	513:517	arg1	litter					519:524	plant litter	513:524	plant litter	513:524	To isolate microbial effects from plant litter effects, we collected plant litter from long-term N fertilized and control plots, reciprocally inoculated it with microbes from the two treatments, and incubated it in a common field setting for three months.
26367868	3	13	theme	plant	478:482	arg1	effects					491:497	plant litter effects	478:497	plant litter effects	478:497	To isolate microbial effects from plant litter effects, we collected plant litter from long-term N fertilized and control plots, reciprocally inoculated it with microbes from the two treatments, and incubated it in a common field setting for three months.
26367868	6	14	theme	microbial	866:874	arg1	origins					887:893	microbial and litter origins	866:893	microbial and litter origins	866:893	We found that microbial and litter origins each contributed to a shift in fungal uptake capacities under N fertilization.
26367868	0	15	theme	Long-Term	74:82	arg1	Enrichment					93:102	Long-Term Nitrogen Enrichment	74:102	Long-Term Nitrogen Enrichment	74:102	Quantum Dots Reveal Shifts in Organic Nitrogen Uptake by Fungi Exposed to Long-Term Nitrogen Enrichment.
26367868	4	16	theme	chitosan	765:772	arg1	uptake					743:748	fungal uptake	736:748	fungal uptake of glycine and chitosan	736:772	We used quantum dots (QDs) to track fungal uptake of glycine and chitosan.
26367868	3	17	theme	litter	484:489	arg1	effects					491:497	plant litter effects	478:497	plant litter effects	478:497	To isolate microbial effects from plant litter effects, we collected plant litter from long-term N fertilized and control plots, reciprocally inoculated it with microbes from the two treatments, and incubated it in a common field setting for three months.
26367868	9	18	theme	N	1338:1338	arg1	fungi					1340:1344	N fungi	1338:1344	N fungi	1338:1344	In particular, control fungi tended to target chitosan only when incubated with control litter, while N fungi targeted glycine regardless of litter type.
26367868	2	19	theme	gene	308:311	arg1	regulation					313:322	gene regulation	308:322	gene regulation	308:322	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	4	20	theme	fungal	736:741	arg1	uptake					743:748	fungal uptake	736:748	fungal uptake of glycine and chitosan	736:772	We used quantum dots (QDs) to track fungal uptake of glycine and chitosan.
26367868	2	21	theme	litter	376:381	arg1	effects					383:389	plant litter effects	370:389	plant litter effects (e.g., changes in composition of N and C compounds)	370:441	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	8	22	theme	N	1207:1207	arg1	origin					1161:1166	litter origin	1154:1166	litter origin	1154:1166	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	8	22	theme	N	1207:1207	arg1	origin					1179:1184	microbial origin	1169:1184	microbial origin	1169:1184	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	8	22	theme	N	1207:1207	arg1	type					1191:1194	type	1191:1194	type	1191:1194	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	1	23	theme	N	153:153	arg1	dynamics					155:162	N dynamics	153:162	N dynamics associated with decomposing plant litter	153:203	Anthropogenic nitrogen (N) enrichment can alter N dynamics associated with decomposing plant litter.
26367868	2	24	theme	N	424:424	arg1	composition					409:419	composition	409:419	composition of N and C compounds	409:440	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	6	25	from	shift	917:921	arg1	capacities					940:949	fungal uptake capacities	926:949	fungal uptake capacities under N fertilization	926:971	We found that microbial and litter origins each contributed to a shift in fungal uptake capacities under N fertilization.
26367868	8	26	theme	organic	1199:1205	arg1	N					1207:1207	organic N	1199:1207	organic N (glycine versus chitosan)	1199:1233	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	10	27	theme	N	1433:1433	arg1	dynamics					1435:1442	N dynamics	1433:1442	N dynamics	1433:1442	Overall, microbial effects may mediate how N dynamics respond to anthropogenic N enrichment in ecosystems.
26367868	2	28	dep	changes	398:404	arg1	e.g.					392:395	e.g.	392:395	e.g.	392:395	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	2	29	dep	changes	297:303	arg1	e.g.					291:294	e.g.	291:294	e.g.	291:294	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	2	30	theme	community	340:348	arg1	composition					350:360	community composition	340:360	community composition	340:360	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	0	31	theme	Quantum	0:6	arg1	Dots					8:11	Quantum Dots	0:11	Quantum Dots	0:11	Quantum Dots Reveal Shifts in Organic Nitrogen Uptake by Fungi Exposed to Long-Term Nitrogen Enrichment.
26367868	3	32	theme	field	668:672	arg1	setting					674:680	a common field setting	659:680	a common field setting for three months	659:697	To isolate microbial effects from plant litter effects, we collected plant litter from long-term N fertilized and control plots, reciprocally inoculated it with microbes from the two treatments, and incubated it in a common field setting for three months.
26367868	6	33	theme	uptake	933:938	arg1	capacities					940:949	fungal uptake capacities	926:949	fungal uptake capacities under N fertilization	926:971	We found that microbial and litter origins each contributed to a shift in fungal uptake capacities under N fertilization.
26367868	7	34	theme	control	1033:1039	arg1	fungi					1041:1045	control fungi	1033:1045	control fungi	1033:1045	Specifically, N fungi preferred glycine over chitosan, but control fungi did not.
26367868	6	35	theme	fungal	926:931	arg1	capacities					940:949	fungal uptake capacities	926:949	fungal uptake capacities under N fertilization	926:971	We found that microbial and litter origins each contributed to a shift in fungal uptake capacities under N fertilization.
26367868	2	36	theme	microbial	272:280	arg1	effects					282:288	microbial effects	272:288	microbial effects (e.g., changes in gene regulation, physiology, or community composition)	272:361	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	1	37	theme	plant	192:196	arg1	litter					198:203	plant litter	192:203	plant litter	192:203	Anthropogenic nitrogen (N) enrichment can alter N dynamics associated with decomposing plant litter.
26367868	0	38	theme	Organic	30:36	arg1	Uptake					47:52	Organic Nitrogen Uptake	30:52	Organic Nitrogen Uptake by Fungi Exposed to Long-Term Nitrogen Enrichment	30:102	Quantum Dots Reveal Shifts in Organic Nitrogen Uptake by Fungi Exposed to Long-Term Nitrogen Enrichment.
26367868	3	39	theme	common	661:666	arg1	setting					674:680	a common field setting	659:680	a common field setting for three months	659:697	To isolate microbial effects from plant litter effects, we collected plant litter from long-term N fertilized and control plots, reciprocally inoculated it with microbes from the two treatments, and incubated it in a common field setting for three months.
26367868	4	40	used	used	703:706	arg2	We					700:701	We	700:701	We	700:701	We used quantum dots (QDs) to track fungal uptake of glycine and chitosan.
26367868	2	41	theme	plant	370:374	arg1	effects					383:389	plant litter effects	370:389	plant litter effects (e.g., changes in composition of N and C compounds)	370:441	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	6	42	theme	N	957:957	arg1	fertilization					959:971	N fertilization	957:971	N fertilization	957:971	We found that microbial and litter origins each contributed to a shift in fungal uptake capacities under N fertilization.
26367868	8	43	theme	litter	1071:1076	arg1	effects					1078:1084	litter effects	1071:1084	litter effects	1071:1084	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	8	43	theme	litter	1071:1076	arg1	interaction					1134:1144	a three-way interaction	1122:1144	a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan)	1122:1233	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	3	44	theme	long-term	531:539	arg1	fertilized					543:552	long-term N fertilized	531:552	long-term N fertilized	531:552	To isolate microbial effects from plant litter effects, we collected plant litter from long-term N fertilized and control plots, reciprocally inoculated it with microbes from the two treatments, and incubated it in a common field setting for three months.
26367868	10	45	from	enrichment	1471:1480	arg1	ecosystems					1485:1494	ecosystems	1485:1494	ecosystems	1485:1494	Overall, microbial effects may mediate how N dynamics respond to anthropogenic N enrichment in ecosystems.
26367868	8	46	theme	three-way	1124:1132	arg1	effects					1078:1084	litter effects	1071:1084	litter effects	1071:1084	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	8	46	theme	three-way	1124:1132	arg1	interaction					1134:1144	a three-way interaction	1122:1144	a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan)	1122:1233	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	3	47	from	treatments	627:636	arg1	microbes					605:612	microbes	605:612	microbes from the two treatments	605:636	To isolate microbial effects from plant litter effects, we collected plant litter from long-term N fertilized and control plots, reciprocally inoculated it with microbes from the two treatments, and incubated it in a common field setting for three months.
26367868	10	48	theme	N	1469:1469	arg1	enrichment					1471:1480	anthropogenic N enrichment	1455:1480	anthropogenic N enrichment in ecosystems	1455:1494	Overall, microbial effects may mediate how N dynamics respond to anthropogenic N enrichment in ecosystems.
26367868	2	49	dep	effects	383:389	arg1	changes					398:404	changes	398:404	changes in composition of N and C compounds	398:440	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	2	50	dep	effects	282:288	arg1	changes					297:303	changes	297:303	changes in gene regulation, physiology, or community composition	297:360	However, it is unclear to what extent these alterations occur via microbial effects (e.g., changes in gene regulation, physiology, or community composition) versus plant litter effects (e.g., changes in composition of N and C compounds).
26367868	0	51	theme	Nitrogen	38:45	arg1	Uptake					47:52	Organic Nitrogen Uptake	30:52	Organic Nitrogen Uptake by Fungi Exposed to Long-Term Nitrogen Enrichment	30:102	Quantum Dots Reveal Shifts in Organic Nitrogen Uptake by Fungi Exposed to Long-Term Nitrogen Enrichment.
26367868	1	52	theme	Anthropogenic	105:117	arg1	N					129:129	N	129:129	N	129:129	Anthropogenic nitrogen (N) enrichment can alter N dynamics associated with decomposing plant litter.
26367868	1	52	theme	Anthropogenic	105:117	arg1	nitrogen					119:126	Anthropogenic nitrogen	105:126	Anthropogenic nitrogen (N) enrichment	105:141	Anthropogenic nitrogen (N) enrichment can alter N dynamics associated with decomposing plant litter.
26367868	1	53	theme	nitrogen	119:126	arg1	enrichment					132:141	Anthropogenic nitrogen (N) enrichment	105:141	Anthropogenic nitrogen (N) enrichment	105:141	Anthropogenic nitrogen (N) enrichment can alter N dynamics associated with decomposing plant litter.
26367868	10	54	theme	anthropogenic	1455:1467	arg1	enrichment					1471:1480	anthropogenic N enrichment	1455:1480	anthropogenic N enrichment in ecosystems	1455:1494	Overall, microbial effects may mediate how N dynamics respond to anthropogenic N enrichment in ecosystems.
26367868	8	55	from	subtle	1096:1101	arg1	comparison					1059:1068	comparison	1059:1068	comparison	1059:1068	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	5	56	theme	N	814:814	arg1	Glycine					775:781	Glycine	775:781	Glycine	775:781	Glycine is a relatively simple organic N compound; chitosan is more complex.
26367868	5	56	theme	N	814:814	arg1	compound					816:823	a relatively simple organic N compound	786:823	a relatively simple organic N compound	786:823	Glycine is a relatively simple organic N compound; chitosan is more complex.
26367868	5	57	theme	simple	799:804	arg1	Glycine					775:781	Glycine	775:781	Glycine	775:781	Glycine is a relatively simple organic N compound; chitosan is more complex.
26367868	5	57	theme	simple	799:804	arg1	compound					816:823	a relatively simple organic N compound	786:823	a relatively simple organic N compound	786:823	Glycine is a relatively simple organic N compound; chitosan is more complex.
26367868	3	58	theme	control	558:564	arg1	plots					566:570	control plots	558:570	control plots	558:570	To isolate microbial effects from plant litter effects, we collected plant litter from long-term N fertilized and control plots, reciprocally inoculated it with microbes from the two treatments, and incubated it in a common field setting for three months.
26367868	8	59	theme	microbial	1169:1177	arg1	origin					1179:1184	microbial origin	1169:1184	microbial origin	1169:1184	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	9	60	theme	control	1316:1322	arg1	litter					1324:1329	control litter	1316:1329	control litter	1316:1329	In particular, control fungi tended to target chitosan only when incubated with control litter, while N fungi targeted glycine regardless of litter type.
26367868	3	61	theme	N	541:541	arg1	fertilized					543:552	long-term N fertilized	531:552	long-term N fertilized	531:552	To isolate microbial effects from plant litter effects, we collected plant litter from long-term N fertilized and control plots, reciprocally inoculated it with microbes from the two treatments, and incubated it in a common field setting for three months.
26367868	8	62	dep	N	1207:1207	arg1	glycine					1210:1216	glycine	1210:1216	glycine	1210:1216	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	8	62	dep	N	1207:1207	arg1	chitosan					1225:1232	chitosan	1225:1232	chitosan	1225:1232	In comparison, litter effects were more subtle, and manifested as a three-way interaction between litter origin, microbial origin, and type of organic N (glycine versus chitosan).
26367868	9	63	theme	litter	1377:1382	arg1	type					1384:1387	litter type	1377:1387	litter type	1377:1387	In particular, control fungi tended to target chitosan only when incubated with control litter, while N fungi targeted glycine regardless of litter type.
26367868	0	64	from	Shifts	20:25	arg1	Uptake					47:52	Organic Nitrogen Uptake	30:52	Organic Nitrogen Uptake by Fungi Exposed to Long-Term Nitrogen Enrichment	30:102	Quantum Dots Reveal Shifts in Organic Nitrogen Uptake by Fungi Exposed to Long-Term Nitrogen Enrichment.
26367868	4	65	theme	quantum	708:714	arg1	QDs					722:724	QDs	722:724	QDs	722:724	We used quantum dots (QDs) to track fungal uptake of glycine and chitosan.
26367868	4	65	theme	quantum	708:714	arg1	dots					716:719	quantum dots	708:719	quantum dots (QDs)	708:725	We used quantum dots (QDs) to track fungal uptake of glycine and chitosan.
26367868	3	66	theme	microbial	455:463	arg1	effects					465:471	microbial effects	455:471	microbial effects	455:471	To isolate microbial effects from plant litter effects, we collected plant litter from long-term N fertilized and control plots, reciprocally inoculated it with microbes from the two treatments, and incubated it in a common field setting for three months.
26367868	10	67	theme	microbial	1399:1407	arg1	effects					1409:1415	microbial effects	1399:1415	microbial effects	1399:1415	Overall, microbial effects may mediate how N dynamics respond to anthropogenic N enrichment in ecosystems.
25877399	0	0	theme	Alginate	74:81	arg1	Distribution					58:69	Monomer Distribution	50:69	Monomer Distribution of Alginate	50:81	Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate.
25877399	3	1	theme	oxygen	297:302	arg1	DOT					313:315	DOT	313:315	DOT	313:315	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	3	1	theme	oxygen	297:302	arg1	tension					304:310	dissolved oxygen tension	287:310	dissolved oxygen tension (DOT)	287:316	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	7	2	theme	%	875:875	arg1	DOT					877:879	10 % DOT	872:879	10 % DOT	872:879	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	7	2	theme	%	875:875	arg1	level					865:869	high DOT level	856:869	high DOT level (10 % DOT)	856:880	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	1	3	theme	guluronic	144:152	arg1	monomers					159:166	mannuronic and guluronic acid monomers	129:166	mannuronic and guluronic acid monomers	129:166	Alginate is a natural biopolymer composed of mannuronic and guluronic acid monomers.
25877399	5	4	from	DOT	672:674	arg1	50 mg/L					646:652	50 mg/L	646:652	50 mg/L of calcium at 5 % DOT	646:674	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	5	4	from	DOT	672:674	arg1	sucrose					634:640	sucrose	634:640	sucrose	634:640	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	5	4	from	DOT	672:674	arg1	20 g/L					624:629	20 g/L	624:629	20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT	624:674	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	3	5	theme	tension	304:310	arg1	effect					277:282	the effect	273:282	the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter	273:473	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	4	6	theme	alginate	496:503	arg1	production					505:514	alginate production	496:514	alginate production	496:514	Results showed that alginate production increased with increasing DOT from 1 to 5 %.
25877399	5	7	theme	highest	565:571	arg1	4.51 g/L					609:616	4.51 g/L	609:616	4.51 g/L	609:616	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	5	7	theme	highest	565:571	arg1	production					582:591	The highest alginate production	561:591	The highest alginate production	561:591	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	1	8	theme	acid	154:157	arg1	monomers					159:166	mannuronic and guluronic acid monomers	129:166	mannuronic and guluronic acid monomers	129:166	Alginate is a natural biopolymer composed of mannuronic and guluronic acid monomers.
25877399	3	9	theme	growth	322:327	arg1	substrate					336:344	growth medium substrate	322:344	growth medium substrate	322:344	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	0	10	from	Effect	0:5	arg1	Distribution					58:69	Monomer Distribution	50:69	Monomer Distribution of Alginate	50:81	Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate.
25877399	5	11	theme	sucrose	634:640	arg1	20 g/L					624:629	20 g/L	624:629	20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT	624:674	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	5	12	theme	alginate	573:580	arg1	4.51 g/L					609:616	4.51 g/L	609:616	4.51 g/L	609:616	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	5	12	theme	alginate	573:580	arg1	production					582:591	The highest alginate production	561:591	The highest alginate production	561:591	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	3	13	theme	medium	329:334	arg1	substrate					336:344	growth medium substrate	322:344	growth medium substrate	322:344	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	7	14	theme	high	856:859	arg1	DOT					877:879	10 % DOT	872:879	10 % DOT	872:879	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	7	14	theme	high	856:859	arg1	level					865:869	high DOT level	856:869	high DOT level (10 % DOT)	856:880	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	7	15	theme	DOT	861:863	arg1	DOT					877:879	10 % DOT	872:879	10 % DOT	872:879	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	7	15	theme	DOT	861:863	arg1	level					865:869	high DOT level	856:869	high DOT level (10 % DOT)	856:880	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	0	16	theme	Medium	29:34	arg1	Components					36:45	Medium Components	29:45	Medium Components	29:45	Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate.
25877399	3	17	theme	calcium	350:356	arg1	concentrations					358:371	calcium concentrations	350:371	calcium concentrations	350:371	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	7	18	theme	extreme	829:835	arg1	level					865:869	high DOT level	856:869	high DOT level (10 % DOT)	856:880	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	7	18	theme	extreme	829:835	arg1	conditions					837:846	extreme conditions	829:846	extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L)	829:919	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	7	18	theme	extreme	829:835	arg1	concentration					898:910	low sucrose concentration	886:910	low sucrose concentration (10 g/L)	886:919	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	6	19	dep	61 	742:744	arg1	to					739:740	to	739:740	to	739:740	At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration.
25877399	3	20	theme	alginate	405:412	arg1	composition					390:400	the monomeric composition	376:400	the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter	376:473	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	3	21	theme	substrate	336:344	arg1	effect					277:282	the effect	273:282	the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter	273:473	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	5	22	theme	5 	668:669	arg1	%					670:670	%	670:670	%	670:670	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	6	23	theme	mannuronic	719:728	arg1	%					745:745	up to 61 %	736:745	up to 61 %	736:745	At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration.
25877399	6	23	theme	mannuronic	719:728	arg1	acid					730:733	mannuronic acid	719:733	mannuronic acid (up to 61 %)	719:746	At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration.
25877399	6	24	from	acid	730:733	arg1	rich					711:714	rich	711:714	rich	711:714	At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration.
25877399	0	25	theme	Oxygen	10:15	arg1	Tension					17:23	Oxygen Tension	10:23	Oxygen Tension	10:23	Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate.
25877399	5	26	theme	%	670:670	arg1	DOT					672:674	5 % DOT	668:674	5 % DOT	668:674	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	6	27	theme	calcium	784:790	arg1	concentration					792:804	low calcium concentration	780:804	low calcium concentration	780:804	At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration.
25877399	6	28	from	conditions	686:695	arg1	rich					711:714	rich	711:714	rich	711:714	At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration.
25877399	3	29	theme	monomeric	380:388	arg1	composition					390:400	the monomeric composition	376:400	the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter	376:473	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	7	30	theme	guluronic	922:930	arg1	acid					932:935	guluronic acid	922:935	guluronic acid	922:935	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	6	31	theme	low	780:782	arg1	concentration					792:804	low calcium concentration	780:804	low calcium concentration	780:804	At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration.
25877399	2	32	theme	Azotobacter	213:223	arg1	algae					187:191	algae	187:191	algae	187:191	It is produced by algae and some species of Azotobacter and Pseudomonas.
25877399	2	32	theme	Azotobacter	213:223	arg1	species					202:208	some species	197:208	some species of Azotobacter and Pseudomonas	197:239	It is produced by algae and some species of Azotobacter and Pseudomonas.
25877399	0	33	theme	Tension	17:23	arg1	Effect					0:5	Effect	0:5	Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate.	0:82	Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate.
25877399	7	34	theme	sucrose	890:896	arg1	10 g/L					913:918	10 g/L	913:918	10 g/L	913:918	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	7	34	theme	sucrose	890:896	arg1	concentration					898:910	low sucrose concentration	886:910	low sucrose concentration (10 g/L)	886:919	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	5	35	theme	50 mg/L	646:652	arg1	20 g/L					624:629	20 g/L	624:629	20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT	624:674	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	4	36	dep	5 	556:557	arg1	to					553:554	to	553:554	to	553:554	Results showed that alginate production increased with increasing DOT from 1 to 5 %.
25877399	1	37	theme	natural	98:104	arg1	Alginate					84:91	Alginate	84:91	Alginate	84:91	Alginate is a natural biopolymer composed of mannuronic and guluronic acid monomers.
25877399	1	37	theme	natural	98:104	arg1	biopolymer					106:115	a natural biopolymer	96:115	a natural biopolymer composed of mannuronic and guluronic acid monomers	96:166	Alginate is a natural biopolymer composed of mannuronic and guluronic acid monomers.
25877399	0	38	theme	Components	36:45	arg1	Effect					0:5	Effect	0:5	Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate.	0:82	Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate.
25877399	5	39	from	20 g/L	624:629	arg1	DOT					672:674	5 % DOT	668:674	5 % DOT	668:674	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	3	40	theme	dissolved	287:295	arg1	DOT					313:315	DOT	313:315	DOT	313:315	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	3	40	theme	dissolved	287:295	arg1	tension					304:310	dissolved oxygen tension	287:310	dissolved oxygen tension (DOT)	287:316	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	7	41	from	conditions	837:846	arg1	dominant					941:948	dominant	941:948	dominant	941:948	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	5	42	theme	calcium	657:663	arg1	50 mg/L					646:652	50 mg/L	646:652	50 mg/L of calcium at 5 % DOT	646:674	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	5	42	theme	calcium	657:663	arg1	sucrose					634:640	sucrose	634:640	sucrose	634:640	The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT.
25877399	7	43	theme	low	886:888	arg1	10 g/L					913:918	10 g/L	913:918	10 g/L	913:918	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	7	43	theme	low	886:888	arg1	concentration					898:910	low sucrose concentration	886:910	low sucrose concentration (10 g/L)	886:919	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	6	44	from	rich	711:714	arg1	%					745:745	up to 61 %	736:745	up to 61 %	736:745	At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration.
25877399	6	44	from	rich	711:714	arg1	acid					730:733	mannuronic acid	719:733	mannuronic acid (up to 61 %)	719:746	At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration.
25877399	7	45	theme	other	814:818	arg1	hand					820:823	the other hand	810:823	the other hand	810:823	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	3	46	from	effect	277:282	arg1	composition					390:400	the monomeric composition	376:400	the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter	376:473	This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter.
25877399	2	47	theme	Pseudomonas	229:239	arg1	algae					187:191	algae	187:191	algae	187:191	It is produced by algae and some species of Azotobacter and Pseudomonas.
25877399	2	47	theme	Pseudomonas	229:239	arg1	species					202:208	some species	197:208	some species of Azotobacter and Pseudomonas	197:239	It is produced by algae and some species of Azotobacter and Pseudomonas.
25877399	1	48	theme	mannuronic	129:138	arg1	monomers					159:166	mannuronic and guluronic acid monomers	129:166	mannuronic and guluronic acid monomers	129:166	Alginate is a natural biopolymer composed of mannuronic and guluronic acid monomers.
25877399	0	49	theme	Monomer	50:56	arg1	Distribution					58:69	Monomer Distribution	50:69	Monomer Distribution of Alginate	50:81	Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate.
25877399	7	50	theme	10 	872:874	arg1	DOT					877:879	10 % DOT	872:879	10 % DOT	872:879	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	7	50	theme	10 	872:874	arg1	level					865:869	high DOT level	856:869	high DOT level (10 % DOT)	856:880	On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).
25877399	6	51	from	concentration	792:804	arg1	high					772:775	high	772:775	high	772:775	At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration.
26478396	0	0	theme	magnesium	80:88	arg1	alloy					90:94	magnesium alloy	80:94	magnesium alloy	80:94	Effect of the addition CNTs on performance of CaP/chitosan/coating deposited on magnesium alloy by electrophoretic deposition.
26478396	3	1	theme	X-ray	498:502	arg1	diffraction					504:514	X-ray diffraction	498:514	X-ray diffraction (XRD)	498:520	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	3	1	theme	X-ray	498:502	arg1	XRD					517:519	XRD	517:519	XRD	517:519	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	6	2	from	amount	1166:1171	arg1	ml					1188:1189	500 ml	1184:1189	500 ml of electrophoretic solution	1184:1217	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	6	3	theme	original	977:984	arg1	morphology					994:1003	their original tubular morphology	971:1003	their original tubular morphology	971:1003	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	6	4	theme	CaP/chitosan	1027:1038	arg1	coating					1040:1046	the CaP/chitosan coating	1023:1046	the CaP/chitosan coating	1023:1046	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	6	5	located	found	1014:1018	arg2	CNTs					961:964	a few CNTs	955:964	a few CNTs with their original tubular morphology	955:1003	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	6	5	located	found	1014:1018	arg1	coating					1040:1046	the CaP/chitosan coating	1023:1046	the CaP/chitosan coating	1023:1046	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	1	6	from	coating	164:170	arg1	alloy					191:195	AZ91D magnesium alloy	175:195	AZ91D magnesium alloy	175:195	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	6	7	theme	phosphate	1099:1107	arg1	growth					1089:1094	the crystal growth	1077:1094	the crystal growth of phosphate and improvement of the coating bonding	1077:1146	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	8	8	theme	based	1453:1457	arg1	CaP/chitosan/CNTs					1459:1475	Mg based CaP/chitosan/CNTs	1450:1475	Mg based CaP/chitosan/CNTs	1450:1475	The cell viability of Mg based CaP/chitosan/CNTs was higher than that of Mg based CaP/chitosan from 16 days to 90 days.
26478396	0	9	from	Effect	0:5	arg1	performance					31:41	performance	31:41	performance of CaP/chitosan/coating	31:65	Effect of the addition CNTs on performance of CaP/chitosan/coating deposited on magnesium alloy by electrophoretic deposition.
26478396	7	10	theme	loading	1251:1257	arg1	gentamicin					1269:1278	gentamicin	1269:1278	gentamicin	1269:1278	The loading amount of gentamicin increased and the releasing speed of gentamicin decreased after CNTs was added into the CaP/chitosan coating for immersion loading and EPD loading.
26478396	7	10	theme	loading	1251:1257	arg1	amount					1259:1264	The loading amount	1247:1264	The loading amount of gentamicin	1247:1278	The loading amount of gentamicin increased and the releasing speed of gentamicin decreased after CNTs was added into the CaP/chitosan coating for immersion loading and EPD loading.
26478396	3	11	theme	Fourier-transformed	523:541	arg1	FTIR					566:569	FTIR	566:569	FTIR	566:569	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	3	11	theme	Fourier-transformed	523:541	arg1	spectroscopy					552:563	Fourier-transformed infrared spectroscopy	523:563	Fourier-transformed infrared spectroscopy (FTIR)	523:570	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	6	12	theme	improvement	1113:1123	arg1	growth					1089:1094	the crystal growth	1077:1094	the crystal growth of phosphate and improvement of the coating bonding	1077:1146	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	5	13	used	used	869:872	arg2	assay					859:863	The cell counting kit (CCK) assay	831:863	The cell counting kit (CCK) assay	831:863	The cell counting kit (CCK) assay was used to evaluate the cytotoxicity of samples to SaOS-2 cells.
26478396	7	14	theme	EPD	1415:1417	arg1	loading					1419:1425	EPD loading	1415:1425	EPD loading	1415:1425	The loading amount of gentamicin increased and the releasing speed of gentamicin decreased after CNTs was added into the CaP/chitosan coating for immersion loading and EPD loading.
26478396	6	15	theme	g	1233:1233	arg1	g					1244:1244	0.05 g to 0.125 g	1228:1244	0.05 g to 0.125 g	1228:1244	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	6	16	theme	CNTs	1176:1179	arg1	CNTs					1176:1179	CNTs	1176:1179	CNTs	1176:1179	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	6	16	theme	CNTs	1176:1179	arg1	amount					1166:1171	the addition amount	1153:1171	the addition amount of CNTs in 500 ml of electrophoretic solution	1153:1217	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	3	17	theme	infrared	543:550	arg1	FTIR					566:569	FTIR	566:569	FTIR	566:569	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	3	17	theme	infrared	543:550	arg1	spectroscopy					552:563	Fourier-transformed infrared spectroscopy	523:563	Fourier-transformed infrared spectroscopy (FTIR)	523:570	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	5	18	theme	SaOS-2	917:922	arg1	cells					924:928	SaOS-2 cells	917:928	SaOS-2 cells	917:928	The cell counting kit (CCK) assay was used to evaluate the cytotoxicity of samples to SaOS-2 cells.
26478396	6	19	dep	g	1244:1244	arg1	to					1235:1236	to	1235:1236	to	1235:1236	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	5	20	theme	kit	849:851	arg1	assay					859:863	The cell counting kit (CCK) assay	831:863	The cell counting kit (CCK) assay	831:863	The cell counting kit (CCK) assay was used to evaluate the cytotoxicity of samples to SaOS-2 cells.
26478396	7	21	theme	CaP/chitosan	1368:1379	arg1	coating					1381:1387	the CaP/chitosan coating	1364:1387	the CaP/chitosan coating for immersion loading and EPD loading	1364:1425	The loading amount of gentamicin increased and the releasing speed of gentamicin decreased after CNTs was added into the CaP/chitosan coating for immersion loading and EPD loading.
26478396	4	22	theme	gentamicin	662:671	arg1	concentration					673:685	gentamicin concentration	662:685	gentamicin concentration	662:685	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	1	23	theme	AZ91D	175:179	arg1	alloy					191:195	AZ91D magnesium alloy	175:195	AZ91D magnesium alloy	175:195	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	0	24	theme	electrophoretic	99:113	arg1	deposition					115:124	electrophoretic deposition	99:124	electrophoretic deposition	99:124	Effect of the addition CNTs on performance of CaP/chitosan/coating deposited on magnesium alloy by electrophoretic deposition.
26478396	8	25	from	days	1531:1534	arg1	CaP/chitosan					1510:1521	Mg based CaP/chitosan	1501:1521	Mg based CaP/chitosan from 16 days to 90 days	1501:1545	The cell viability of Mg based CaP/chitosan/CNTs was higher than that of Mg based CaP/chitosan from 16 days to 90 days.
26478396	6	26	theme	bonding	1140:1146	arg1	improvement					1113:1123	improvement	1113:1123	improvement of the coating bonding	1113:1146	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	6	26	theme	bonding	1140:1146	arg1	phosphate					1099:1107	phosphate	1099:1107	phosphate	1099:1107	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	1	27	theme	magnesium	181:189	arg1	alloy					191:195	AZ91D magnesium alloy	175:195	AZ91D magnesium alloy	175:195	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	6	28	theme	coating	1132:1138	arg1	bonding					1140:1146	the coating bonding	1128:1146	the coating bonding	1128:1146	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	6	29	theme	few	957:959	arg1	CNTs					961:964	a few CNTs	955:964	a few CNTs with their original tubular morphology	955:1003	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	4	30	theme	emission	754:761	arg1	spectrometer					763:774	inductively coupled plasma optical emission spectrometer	719:774	inductively coupled plasma optical emission spectrometer (ICP-OES) test	719:789	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	4	30	theme	emission	754:761	arg1	ICP-OES					777:783	ICP-OES	777:783	ICP-OES	777:783	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	3	31	theme	phase	408:412	arg1	compositions					414:425	The phase compositions	404:425	The phase compositions	404:425	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	8	32	theme	CaP/chitosan/CNTs	1459:1475	arg1	higher					1481:1486	higher	1481:1486	higher	1481:1486	The cell viability of Mg based CaP/chitosan/CNTs was higher than that of Mg based CaP/chitosan from 16 days to 90 days.
26478396	8	32	theme	CaP/chitosan/CNTs	1459:1475	arg1	viability					1437:1445	The cell viability	1428:1445	The cell viability of Mg based CaP/chitosan/CNTs	1428:1475	The cell viability of Mg based CaP/chitosan/CNTs was higher than that of Mg based CaP/chitosan from 16 days to 90 days.
26478396	1	33	theme	phosphate	275:283	arg1	solution					292:299	a phosphate buffer solution	273:299	a phosphate buffer solution (PBS)	273:305	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	1	33	theme	phosphate	275:283	arg1	PBS					302:304	PBS	302:304	PBS	302:304	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	6	34	theme	solution	1210:1217	arg1	ml					1188:1189	500 ml	1184:1189	500 ml of electrophoretic solution	1184:1217	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	4	35	theme	optical	746:752	arg1	spectrometer					763:774	inductively coupled plasma optical emission spectrometer	719:774	inductively coupled plasma optical emission spectrometer (ICP-OES) test	719:789	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	4	35	theme	optical	746:752	arg1	ICP-OES					777:783	ICP-OES	777:783	ICP-OES	777:783	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	7	36	theme	immersion	1393:1401	arg1	loading					1403:1409	immersion loading	1393:1409	immersion loading	1393:1409	The loading amount of gentamicin increased and the releasing speed of gentamicin decreased after CNTs was added into the CaP/chitosan coating for immersion loading and EPD loading.
26478396	0	37	theme	addition	14:21	arg1	CNTs					23:26	the addition CNTs	10:26	the addition CNTs	10:26	Effect of the addition CNTs on performance of CaP/chitosan/coating deposited on magnesium alloy by electrophoretic deposition.
26478396	1	38	theme	buffer	285:290	arg1	solution					292:299	a phosphate buffer solution	273:299	a phosphate buffer solution (PBS)	273:305	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	1	38	theme	buffer	285:290	arg1	PBS					302:304	PBS	302:304	PBS	302:304	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	6	39	with	CNTs	961:964	arg1	morphology					994:1003	their original tubular morphology	971:1003	their original tubular morphology	971:1003	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	3	40	theme	composite	454:462	arg1	coatings					464:471	the composite coatings	450:471	the composite coatings	450:471	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	5	41	theme	cell	835:838	arg1	assay					859:863	The cell counting kit (CCK) assay	831:863	The cell counting kit (CCK) assay	831:863	The cell counting kit (CCK) assay was used to evaluate the cytotoxicity of samples to SaOS-2 cells.
26478396	6	42	theme	addition	1157:1164	arg1	CNTs					1176:1179	CNTs	1176:1179	CNTs	1176:1179	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	6	42	theme	addition	1157:1164	arg1	amount					1166:1171	the addition amount	1153:1171	the addition amount of CNTs in 500 ml of electrophoretic solution	1153:1217	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	4	43	theme	spectrometer	763:774	arg1	test					786:789	inductively coupled plasma optical emission spectrometer (ICP-OES) test	719:789	inductively coupled plasma optical emission spectrometer (ICP-OES) test	719:789	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	3	44	theme	coatings	464:471	arg1	microstructures					431:445	microstructures	431:445	microstructures	431:445	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	3	44	theme	coatings	464:471	arg1	compositions					414:425	The phase compositions	404:425	The phase compositions	404:425	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	5	45	theme	samples	906:912	arg1	cytotoxicity					890:901	the cytotoxicity	886:901	the cytotoxicity of samples to SaOS-2 cells	886:928	The cell counting kit (CCK) assay was used to evaluate the cytotoxicity of samples to SaOS-2 cells.
26478396	8	46	theme	cell	1432:1435	arg1	higher					1481:1486	higher	1481:1486	higher	1481:1486	The cell viability of Mg based CaP/chitosan/CNTs was higher than that of Mg based CaP/chitosan from 16 days to 90 days.
26478396	8	46	theme	cell	1432:1435	arg1	viability					1437:1445	The cell viability	1428:1445	The cell viability of Mg based CaP/chitosan/CNTs	1428:1475	The cell viability of Mg based CaP/chitosan/CNTs was higher than that of Mg based CaP/chitosan from 16 days to 90 days.
26478396	6	47	theme	electrophoretic	1194:1208	arg1	solution					1210:1217	electrophoretic solution	1194:1217	electrophoretic solution	1194:1217	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	7	48	theme	gentamicin	1269:1278	arg1	gentamicin					1269:1278	gentamicin	1269:1278	gentamicin	1269:1278	The loading amount of gentamicin increased and the releasing speed of gentamicin decreased after CNTs was added into the CaP/chitosan coating for immersion loading and EPD loading.
26478396	7	48	theme	gentamicin	1269:1278	arg1	amount					1259:1264	The loading amount	1247:1264	The loading amount of gentamicin	1247:1278	The loading amount of gentamicin increased and the releasing speed of gentamicin decreased after CNTs was added into the CaP/chitosan coating for immersion loading and EPD loading.
26478396	1	49	theme	electrophoretic	214:228	arg1	EPD					242:244	EPD	242:244	EPD	242:244	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	1	49	theme	electrophoretic	214:228	arg1	deposition					230:239	electrophoretic deposition	214:239	electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS)	214:305	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	2	50	theme	automatic	374:382	arg1	instrument					392:401	an automatic scratch instrument	371:401	an automatic scratch instrument	371:401	The bonding between the layer and the substrate was studied by an automatic scratch instrument.
26478396	0	51	theme	CNTs	23:26	arg1	Effect					0:5	Effect	0:5	Effect of the addition CNTs on performance of CaP/chitosan/coating	0:65	Effect of the addition CNTs on performance of CaP/chitosan/coating deposited on magnesium alloy by electrophoretic deposition.
26478396	2	52	theme	scratch	384:390	arg1	instrument					392:401	an automatic scratch instrument	371:401	an automatic scratch instrument	371:401	The bonding between the layer and the substrate was studied by an automatic scratch instrument.
26478396	7	53	theme	gentamicin	1317:1326	arg1	speed					1308:1312	the releasing speed	1294:1312	the releasing speed of gentamicin decreased after CNTs	1294:1347	The loading amount of gentamicin increased and the releasing speed of gentamicin decreased after CNTs was added into the CaP/chitosan coating for immersion loading and EPD loading.
26478396	1	54	from	conversion	259:268	arg1	solution					292:299	a phosphate buffer solution	273:299	a phosphate buffer solution (PBS)	273:305	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	1	54	from	conversion	259:268	arg1	PBS					302:304	PBS	302:304	PBS	302:304	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	8	55	theme	based	1504:1508	arg1	CaP/chitosan					1510:1521	Mg based CaP/chitosan	1501:1521	Mg based CaP/chitosan from 16 days to 90 days	1501:1545	The cell viability of Mg based CaP/chitosan/CNTs was higher than that of Mg based CaP/chitosan from 16 days to 90 days.
26478396	3	56	theme	Raman	573:577	arg1	spectroscopy					579:590	Raman spectroscopy	573:590	Raman spectroscopy	573:590	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	1	57	theme	CaP/chitosan/carbon	127:145	arg1	nanotubes					147:155	CaP/chitosan/carbon nanotubes	127:155	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy	127:195	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	1	57	theme	CaP/chitosan/carbon	127:145	arg1	CNTs					158:161	CNTs	158:161	CNTs	158:161	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	7	58	theme	releasing	1298:1306	arg1	speed					1308:1312	the releasing speed	1294:1312	the releasing speed of gentamicin decreased after CNTs	1294:1347	The loading amount of gentamicin increased and the releasing speed of gentamicin decreased after CNTs was added into the CaP/chitosan coating for immersion loading and EPD loading.
26478396	8	59	theme	Mg	1501:1502	arg1	CaP/chitosan					1510:1521	Mg based CaP/chitosan	1501:1521	Mg based CaP/chitosan from 16 days to 90 days	1501:1545	The cell viability of Mg based CaP/chitosan/CNTs was higher than that of Mg based CaP/chitosan from 16 days to 90 days.
26478396	1	60	theme	nanotubes	147:155	arg1	coating					164:170	CaP/chitosan/carbon nanotubes (CNTs) coating	127:170	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy	127:195	CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS).
26478396	4	61	theme	ultraviolet	795:805	arg1	UV					826:827	UV	826:827	UV	826:827	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	4	61	theme	ultraviolet	795:805	arg1	spectrophotometer					807:823	ultraviolet spectrophotometer	795:823	ultraviolet spectrophotometer (UV)	795:828	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	4	62	theme	coupled	731:737	arg1	spectrometer					763:774	inductively coupled plasma optical emission spectrometer	719:774	inductively coupled plasma optical emission spectrometer (ICP-OES) test	719:789	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	4	62	theme	coupled	731:737	arg1	ICP-OES					777:783	ICP-OES	777:783	ICP-OES	777:783	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	4	63	theme	plasma	739:744	arg1	spectrometer					763:774	inductively coupled plasma optical emission spectrometer	719:774	inductively coupled plasma optical emission spectrometer (ICP-OES) test	719:789	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	4	63	theme	plasma	739:744	arg1	ICP-OES					777:783	ICP-OES	777:783	ICP-OES	777:783	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	5	64	theme	CCK	854:856	arg1	assay					859:863	The cell counting kit (CCK) assay	831:863	The cell counting kit (CCK) assay	831:863	The cell counting kit (CCK) assay was used to evaluate the cytotoxicity of samples to SaOS-2 cells.
26478396	3	65	theme	scanning	596:603	arg1	SEM					626:628	SEM	626:628	SEM	626:628	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	3	65	theme	scanning	596:603	arg1	microscope					614:623	scanning electron microscope	596:623	scanning electron microscope (SEM)	596:629	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	5	66	theme	counting	840:847	arg1	assay					859:863	The cell counting kit (CCK) assay	831:863	The cell counting kit (CCK) assay	831:863	The cell counting kit (CCK) assay was used to evaluate the cytotoxicity of samples to SaOS-2 cells.
26478396	6	67	theme	crystal	1081:1087	arg1	growth					1089:1094	the crystal growth	1077:1094	the crystal growth of phosphate and improvement of the coating bonding	1077:1146	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
26478396	4	68	theme	element	636:642	arg1	concentration					644:656	The element concentration	632:656	The element concentration	632:656	The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV).
26478396	0	69	theme	CaP/chitosan/coating	46:65	arg1	performance					31:41	performance	31:41	performance of CaP/chitosan/coating	31:65	Effect of the addition CNTs on performance of CaP/chitosan/coating deposited on magnesium alloy by electrophoretic deposition.
26478396	8	70	theme	Mg	1450:1451	arg1	CaP/chitosan/CNTs					1459:1475	Mg based CaP/chitosan/CNTs	1450:1475	Mg based CaP/chitosan/CNTs	1450:1475	The cell viability of Mg based CaP/chitosan/CNTs was higher than that of Mg based CaP/chitosan from 16 days to 90 days.
26478396	3	71	theme	electron	605:612	arg1	SEM					626:628	SEM	626:628	SEM	626:628	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	3	71	theme	electron	605:612	arg1	microscope					614:623	scanning electron microscope	596:623	scanning electron microscope (SEM)	596:629	The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM).
26478396	6	72	theme	tubular	986:992	arg1	morphology					994:1003	their original tubular morphology	971:1003	their original tubular morphology	971:1003	The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g.
28554546	9	0	theme	complexes	1310:1318	arg1	formation					1287:1295	the formation	1283:1295	the formation of inclusion complexes within the tridimensional CD-MOF structures	1283:1362	Raman spectra of individual particles were recorded, confirming the formation of inclusion complexes within the tridimensional CD-MOF structures.
28554546	5	1	theme	around	869:874	arg1	6μm					876:878	around 6μm	869:878	around 6μm	869:878	The crystalline particles, loaded or not with LPZ, have mean diameters of around 6μm.
28554546	4	2	theme	cubic	737:741	arg1	morphologies					743:754	almost perfect cubic morphologies	722:754	almost perfect cubic morphologies	722:754	They were characterized in terms of morphology, size and crystallinity, showing almost perfect cubic morphologies with monodispersed size distributions.
28554546	5	3	theme	crystalline	799:809	arg1	particles					811:819	The crystalline particles	795:819	The crystalline particles	795:819	The crystalline particles, loaded or not with LPZ, have mean diameters of around 6μm.
28554546	3	4	with	assembly	550:557	arg1	γ-CD					564:567	γ-CD	564:567	γ-CD	564:567	Herein, LPZ loaded CD-MOFs were successfully synthesized upon the assembly with γ-CD in the presence of K+ ions using an optimized co-crystallization method.
28554546	4	5	theme	perfect	729:735	arg1	morphologies					743:754	almost perfect cubic morphologies	722:754	almost perfect cubic morphologies	722:754	They were characterized in terms of morphology, size and crystallinity, showing almost perfect cubic morphologies with monodispersed size distributions.
28554546	10	6	theme	individual	1397:1406	arg1	particle					1408:1415	each individual particle	1392:1415	each individual particle	1392:1415	Of note, it was found that each individual particle had the same chemical composition.
28554546	11	7	theme	size	1538:1541	arg1	terms					1507:1511	terms	1507:1511	terms of both drug loading and size	1507:1541	The LPZ-loaded particles had remarkable homogeneity in terms of both drug loading and size.
28554546	1	8	theme	drug	316:319	arg1	delivery					321:328	drug delivery	316:328	drug delivery	316:328	Cyclodextrin-based metal-organic frameworks (CD-MOFs) represent an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery.
28554546	0	9	theme	morphology	103:112	arg1	Study					94:98	Study	94:98	Study of morphology	94:112	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	9	theme	morphology	103:112	arg1	particles					44:52	Cyclodextrin-based metal-organic frameworks particles	0:52	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.	0:162	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	9	theme	morphology	103:112	arg1	composition					127:137	chemical composition	118:137	chemical composition of individual particles	118:161	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	9	10	theme	tridimensional	1331:1344	arg1	structures					1353:1362	the tridimensional CD-MOF structures	1327:1362	the tridimensional CD-MOF structures	1327:1362	Raman spectra of individual particles were recorded, confirming the formation of inclusion complexes within the tridimensional CD-MOF structures.
28554546	9	11	theme	CD-MOF	1346:1351	arg1	structures					1353:1362	the tridimensional CD-MOF structures	1327:1362	the tridimensional CD-MOF structures	1327:1362	Raman spectra of individual particles were recorded, confirming the formation of inclusion complexes within the tridimensional CD-MOF structures.
28554546	7	12	theme	spectroscopic	1092:1104	arg1	characteristics					1106:1120	its spectroscopic characteristics	1088:1120	its spectroscopic characteristics	1088:1120	It was demonstrated that even after two years storage, the incorporated drug inside the CD-MOFs maintained its spectroscopic characteristics.
28554546	5	13	theme	6μm	876:878	arg1	diameters					856:864	mean diameters	851:864	mean diameters of around 6μm	851:878	The crystalline particles, loaded or not with LPZ, have mean diameters of around 6μm.
28554546	0	14	theme	chemical	118:125	arg1	particles					44:52	Cyclodextrin-based metal-organic frameworks particles	0:52	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.	0:162	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	14	theme	chemical	118:125	arg1	composition					127:137	chemical composition	118:137	chemical composition of individual particles	118:161	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	11	15	contain	had	1477:1479	arg1	particles					1467:1475	The LPZ-loaded particles	1452:1475	The LPZ-loaded particles	1452:1475	The LPZ-loaded particles had remarkable homogeneity in terms of both drug loading and size.
28554546	11	15	contain	had	1477:1479	arg2	homogeneity					1492:1502	remarkable homogeneity	1481:1502	remarkable homogeneity in terms of both drug loading and size	1481:1541	The LPZ-loaded particles had remarkable homogeneity in terms of both drug loading and size.
28554546	2	16	from	production	396:405	arg1	stomach					422:428	the stomach	418:428	the stomach	418:428	Lansoprazole (LPZ) is a proton-pump inhibitor used to reduce the production of acid in the stomach and recently identified as an antitubercular prodrug.
28554546	5	17	theme	mean	851:854	arg1	diameters					856:864	mean diameters	851:864	mean diameters of around 6μm	851:878	The crystalline particles, loaded or not with LPZ, have mean diameters of around 6μm.
28554546	4	18	theme	size	690:693	arg1	terms					669:673	terms	669:673	terms of morphology, size and crystallinity	669:711	They were characterized in terms of morphology, size and crystallinity, showing almost perfect cubic morphologies with monodispersed size distributions.
28554546	0	19	theme	metal-organic	19:31	arg1	Study					94:98	Study	94:98	Study of morphology	94:112	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	19	theme	metal-organic	19:31	arg1	particles					44:52	Cyclodextrin-based metal-organic frameworks particles	0:52	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.	0:162	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	19	theme	metal-organic	19:31	arg1	composition					127:137	chemical composition	118:137	chemical composition of individual particles	118:161	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	2	20	theme	proton-pump	355:365	arg1	inhibitor					367:375	a proton-pump inhibitor	353:375	a proton-pump inhibitor used to reduce the production of acid in the stomach and recently identified as an antitubercular prodrug	353:481	Lansoprazole (LPZ) is a proton-pump inhibitor used to reduce the production of acid in the stomach and recently identified as an antitubercular prodrug.
28554546	2	20	theme	proton-pump	355:365	arg1	Lansoprazole					331:342	Lansoprazole	331:342	Lansoprazole (LPZ)	331:348	Lansoprazole (LPZ) is a proton-pump inhibitor used to reduce the production of acid in the stomach and recently identified as an antitubercular prodrug.
28554546	11	21	theme	LPZ-loaded	1456:1465	arg1	particles					1467:1475	The LPZ-loaded particles	1452:1475	The LPZ-loaded particles	1452:1475	The LPZ-loaded particles had remarkable homogeneity in terms of both drug loading and size.
28554546	0	22	theme	Cyclodextrin-based	0:17	arg1	Study					94:98	Study	94:98	Study of morphology	94:112	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	22	theme	Cyclodextrin-based	0:17	arg1	particles					44:52	Cyclodextrin-based metal-organic frameworks particles	0:52	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.	0:162	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	22	theme	Cyclodextrin-based	0:17	arg1	composition					127:137	chemical composition	118:137	chemical composition of individual particles	118:161	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	10	23	theme	chemical	1430:1437	arg1	composition					1439:1449	the same chemical composition	1421:1449	the same chemical composition	1421:1449	Of note, it was found that each individual particle had the same chemical composition.
28554546	9	24	theme	inclusion	1300:1308	arg1	complexes					1310:1318	inclusion complexes	1300:1318	inclusion complexes	1300:1318	Raman spectra of individual particles were recorded, confirming the formation of inclusion complexes within the tridimensional CD-MOF structures.
28554546	4	25	theme	crystallinity	699:711	arg1	terms					669:673	terms	669:673	terms of morphology, size and crystallinity	669:711	They were characterized in terms of morphology, size and crystallinity, showing almost perfect cubic morphologies with monodispersed size distributions.
28554546	0	26	theme	individual	142:151	arg1	particles					153:161	individual particles	142:161	individual particles	142:161	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	9	27	theme	individual	1236:1245	arg1	particles					1247:1255	individual particles	1236:1255	individual particles	1236:1255	Raman spectra of individual particles were recorded, confirming the formation of inclusion complexes within the tridimensional CD-MOF structures.
28554546	3	28	theme	K+	588:589	arg1	ions					591:594	K+ ions	588:594	K+ ions using an optimized co-crystallization method	588:639	Herein, LPZ loaded CD-MOFs were successfully synthesized upon the assembly with γ-CD in the presence of K+ ions using an optimized co-crystallization method.
28554546	1	29	theme	environment-friendly	231:250	arg1	class					270:274	an environment-friendly and biocompatible class	228:274	an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery	228:328	Cyclodextrin-based metal-organic frameworks (CD-MOFs) represent an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery.
28554546	2	30	theme	antitubercular	460:473	arg1	prodrug					475:481	an antitubercular prodrug	457:481	an antitubercular prodrug	457:481	Lansoprazole (LPZ) is a proton-pump inhibitor used to reduce the production of acid in the stomach and recently identified as an antitubercular prodrug.
28554546	9	31	theme	particles	1247:1255	arg1	spectra					1225:1231	Raman spectra	1219:1231	Raman spectra of individual particles	1219:1255	Raman spectra of individual particles were recorded, confirming the formation of inclusion complexes within the tridimensional CD-MOF structures.
28554546	3	32	theme	ions	591:594	arg1	presence					576:583	the presence	572:583	the presence of K+ ions using an optimized co-crystallization method	572:639	Herein, LPZ loaded CD-MOFs were successfully synthesized upon the assembly with γ-CD in the presence of K+ ions using an optimized co-crystallization method.
28554546	11	33	theme	remarkable	1481:1490	arg1	homogeneity					1492:1502	remarkable homogeneity	1481:1502	remarkable homogeneity in terms of both drug loading and size	1481:1541	The LPZ-loaded particles had remarkable homogeneity in terms of both drug loading and size.
28554546	1	34	theme	Cyclodextrin-based	164:181	arg1	CD-MOFs					209:215	CD-MOFs	209:215	CD-MOFs	209:215	Cyclodextrin-based metal-organic frameworks (CD-MOFs) represent an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery.
28554546	1	34	theme	Cyclodextrin-based	164:181	arg1	frameworks					197:206	Cyclodextrin-based metal-organic frameworks	164:206	Cyclodextrin-based metal-organic frameworks (CD-MOFs)	164:216	Cyclodextrin-based metal-organic frameworks (CD-MOFs) represent an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery.
28554546	0	35	theme	efficient	57:65	arg1	carriers					67:74	efficient carriers	57:74	efficient carriers for lansoprazole	57:91	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	1	36	theme	biocompatible	256:268	arg1	class					270:274	an environment-friendly and biocompatible class	228:274	an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery	228:328	Cyclodextrin-based metal-organic frameworks (CD-MOFs) represent an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery.
28554546	0	37	theme	particles	153:161	arg1	Study					94:98	Study	94:98	Study of morphology	94:112	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	37	theme	particles	153:161	arg1	particles					44:52	Cyclodextrin-based metal-organic frameworks particles	0:52	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.	0:162	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	37	theme	particles	153:161	arg1	composition					127:137	chemical composition	118:137	chemical composition of individual particles	118:161	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	11	38	from	homogeneity	1492:1502	arg1	terms					1507:1511	terms	1507:1511	terms of both drug loading and size	1507:1541	The LPZ-loaded particles had remarkable homogeneity in terms of both drug loading and size.
28554546	4	39	theme	morphology	678:687	arg1	terms					669:673	terms	669:673	terms of morphology, size and crystallinity	669:711	They were characterized in terms of morphology, size and crystallinity, showing almost perfect cubic morphologies with monodispersed size distributions.
28554546	6	40	theme	1:1	955:957	arg1	ratio					946:950	a molar ratio	938:950	a molar ratio of 1:1 between LPZ and γ-CD	938:978	The payloads reached 23.2±2.1% (wt) which corresponds to a molar ratio of 1:1 between LPZ and γ-CD.
28554546	8	41	theme	Molecular	1123:1131	arg1	modelling					1133:1141	Molecular modelling	1123:1141	Molecular modelling	1123:1141	Molecular modelling provided a deeper insight into the interaction between the LPZ and CD-MOFs.
28554546	5	42	contain	have	846:849	arg2	diameters					856:864	mean diameters	851:864	mean diameters of around 6μm	851:878	The crystalline particles, loaded or not with LPZ, have mean diameters of around 6μm.
28554546	5	42	contain	have	846:849	arg1	particles					811:819	The crystalline particles	795:819	The crystalline particles	795:819	The crystalline particles, loaded or not with LPZ, have mean diameters of around 6μm.
28554546	3	43	theme	optimized	605:613	arg1	method					634:639	an optimized co-crystallization method	602:639	an optimized co-crystallization method	602:639	Herein, LPZ loaded CD-MOFs were successfully synthesized upon the assembly with γ-CD in the presence of K+ ions using an optimized co-crystallization method.
28554546	7	44	theme	years	1021:1025	arg1	storage					1027:1033	two years storage	1017:1033	two years storage	1017:1033	It was demonstrated that even after two years storage, the incorporated drug inside the CD-MOFs maintained its spectroscopic characteristics.
28554546	12	45	theme	delivery	1607:1614	arg1	purposes					1616:1623	drug delivery purposes	1602:1623	drug delivery purposes	1602:1623	These results pave the way towards the use of CD-MOFs for drug delivery purposes.
28554546	7	46	theme	incorporated	1040:1051	arg1	drug					1053:1056	the incorporated drug	1036:1056	the incorporated drug inside the CD-MOFs	1036:1075	It was demonstrated that even after two years storage, the incorporated drug inside the CD-MOFs maintained its spectroscopic characteristics.
28554546	3	47	theme	co-crystallization	615:632	arg1	method					634:639	an optimized co-crystallization method	602:639	an optimized co-crystallization method	602:639	Herein, LPZ loaded CD-MOFs were successfully synthesized upon the assembly with γ-CD in the presence of K+ ions using an optimized co-crystallization method.
28554546	11	48	theme	drug	1521:1524	arg1	loading					1526:1532	drug loading	1521:1532	drug loading	1521:1532	The LPZ-loaded particles had remarkable homogeneity in terms of both drug loading and size.
28554546	10	49	theme	same	1425:1428	arg1	composition					1439:1449	the same chemical composition	1421:1449	the same chemical composition	1421:1449	Of note, it was found that each individual particle had the same chemical composition.
28554546	9	50	theme	Raman	1219:1223	arg1	spectra					1225:1231	Raman spectra	1219:1231	Raman spectra of individual particles	1219:1255	Raman spectra of individual particles were recorded, confirming the formation of inclusion complexes within the tridimensional CD-MOF structures.
28554546	0	51	dep	particles	44:52	arg1	Study					94:98	Study	94:98	Study of morphology	94:112	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	51	dep	particles	44:52	arg1	particles					44:52	Cyclodextrin-based metal-organic frameworks particles	0:52	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.	0:162	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	51	dep	particles	44:52	arg1	composition					127:137	chemical composition	118:137	chemical composition of individual particles	118:161	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	4	52	theme	size	775:778	arg1	distributions					780:792	monodispersed size distributions	761:792	monodispersed size distributions	761:792	They were characterized in terms of morphology, size and crystallinity, showing almost perfect cubic morphologies with monodispersed size distributions.
28554546	11	53	theme	loading	1526:1532	arg1	terms					1507:1511	terms	1507:1511	terms of both drug loading and size	1507:1541	The LPZ-loaded particles had remarkable homogeneity in terms of both drug loading and size.
28554546	8	54	dep	LPZ	1202:1204	arg1	the					1198:1200	the	1198:1200	the	1198:1200	Molecular modelling provided a deeper insight into the interaction between the LPZ and CD-MOFs.
28554546	12	55	theme	CD-MOFs	1590:1596	arg1	use					1583:1585	the use	1579:1585	the use of CD-MOFs	1579:1596	These results pave the way towards the use of CD-MOFs for drug delivery purposes.
28554546	4	56	theme	monodispersed	761:773	arg1	distributions					780:792	monodispersed size distributions	761:792	monodispersed size distributions	761:792	They were characterized in terms of morphology, size and crystallinity, showing almost perfect cubic morphologies with monodispersed size distributions.
28554546	12	57	theme	drug	1602:1605	arg1	purposes					1616:1623	drug delivery purposes	1602:1623	drug delivery purposes	1602:1623	These results pave the way towards the use of CD-MOFs for drug delivery purposes.
28554546	1	58	theme	MOFs	279:282	arg1	class					270:274	an environment-friendly and biocompatible class	228:274	an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery	228:328	Cyclodextrin-based metal-organic frameworks (CD-MOFs) represent an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery.
28554546	8	59	theme	deeper	1154:1159	arg1	insight					1161:1167	a deeper insight	1152:1167	a deeper insight into the interaction between the LPZ and CD-MOFs	1152:1216	Molecular modelling provided a deeper insight into the interaction between the LPZ and CD-MOFs.
28554546	3	60	theme	loaded	496:501	arg1	CD-MOFs					503:509	LPZ loaded CD-MOFs	492:509	LPZ loaded CD-MOFs	492:509	Herein, LPZ loaded CD-MOFs were successfully synthesized upon the assembly with γ-CD in the presence of K+ ions using an optimized co-crystallization method.
28554546	2	61	theme	acid	410:413	arg1	production					396:405	the production	392:405	the production of acid in the stomach	392:428	Lansoprazole (LPZ) is a proton-pump inhibitor used to reduce the production of acid in the stomach and recently identified as an antitubercular prodrug.
28554546	10	62	contain	had	1417:1419	arg2	composition					1439:1449	the same chemical composition	1421:1449	the same chemical composition	1421:1449	Of note, it was found that each individual particle had the same chemical composition.
28554546	10	62	contain	had	1417:1419	arg1	particle					1408:1415	each individual particle	1392:1415	each individual particle	1392:1415	Of note, it was found that each individual particle had the same chemical composition.
28554546	6	63	theme	molar	940:944	arg1	ratio					946:950	a molar ratio	938:950	a molar ratio of 1:1 between LPZ and γ-CD	938:978	The payloads reached 23.2±2.1% (wt) which corresponds to a molar ratio of 1:1 between LPZ and γ-CD.
28554546	0	64	theme	frameworks	33:42	arg1	Study					94:98	Study	94:98	Study of morphology	94:112	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	64	theme	frameworks	33:42	arg1	particles					44:52	Cyclodextrin-based metal-organic frameworks particles	0:52	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.	0:162	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	0	64	theme	frameworks	33:42	arg1	composition					127:137	chemical composition	118:137	chemical composition of individual particles	118:161	Cyclodextrin-based metal-organic frameworks particles as efficient carriers for lansoprazole: Study of morphology and chemical composition of individual particles.
28554546	1	65	theme	metal-organic	183:195	arg1	CD-MOFs					209:215	CD-MOFs	209:215	CD-MOFs	209:215	Cyclodextrin-based metal-organic frameworks (CD-MOFs) represent an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery.
28554546	1	65	theme	metal-organic	183:195	arg1	frameworks					197:206	Cyclodextrin-based metal-organic frameworks	164:206	Cyclodextrin-based metal-organic frameworks (CD-MOFs)	164:216	Cyclodextrin-based metal-organic frameworks (CD-MOFs) represent an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery.
28554546	1	66	theme	increasing	292:301	arg1	attention					303:311	increasing attention	292:311	increasing attention	292:311	Cyclodextrin-based metal-organic frameworks (CD-MOFs) represent an environment-friendly and biocompatible class of MOFs drawing increasing attention in drug delivery.
29235238	4	0	theme	phosphate	558:566	arg1	PBS					585:587	PBS	585:587	PBS	585:587	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	4	0	theme	phosphate	558:566	arg1	solution					575:582	pH 7.4 phosphate buffer solution	551:582	pH 7.4 phosphate buffer solution (PBS)	551:588	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	3	1	theme	resulting	485:493	arg1	nanoparticles					505:517	the resulting composite nanoparticles	481:517	the resulting composite nanoparticles	481:517	And, the good pore forming effect of Chitosan was introduced into SiO2 nanoparticles, and the resulting composite nanoparticles also had a porous structure.
29235238	1	2	theme	composite	245:253	arg1	nanoparticles					255:267	PEI/Chitosan/SiO2 composite nanoparticles	227:267	PEI/Chitosan/SiO2 composite nanoparticles	227:267	In this paper, polyethylenimine (PEI) and Chitosan were simultaneously one-step doped into silicon dioxide (SiO2 ) nanoparticles to synthesize PEI/Chitosan/SiO2 composite nanoparticles.
29235238	5	3	theme	sensitive	880:888	arg1	assembly					903:910	a high sensitive controllable assembly	873:910	a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials	873:1014	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	3	4	theme	composite	495:503	arg1	nanoparticles					505:517	the resulting composite nanoparticles	481:517	the resulting composite nanoparticles	481:517	And, the good pore forming effect of Chitosan was introduced into SiO2 nanoparticles, and the resulting composite nanoparticles also had a porous structure.
29235238	4	5	theme	buffer	568:573	arg1	PBS					585:587	PBS	585:587	PBS	585:587	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	4	5	theme	buffer	568:573	arg1	solution					575:582	pH 7.4 phosphate buffer solution	551:582	pH 7.4 phosphate buffer solution (PBS)	551:588	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	6	6	theme	sensing	1104:1110	arg1	mechanism					1112:1120	the sensing mechanism	1100:1120	the sensing mechanism	1100:1120	The factors affecting the sensing performance of the sensor were investigated, and the sensing mechanism was also further studied.
29235238	3	7	theme	porous	530:535	arg1	structure					537:545	a porous structure	528:545	a porous structure	528:545	And, the good pore forming effect of Chitosan was introduced into SiO2 nanoparticles, and the resulting composite nanoparticles also had a porous structure.
29235238	5	8	theme	fluorescent	925:935	arg1	sensor					937:942	DNA gated fluorescent sensor	915:942	DNA gated fluorescent sensor	915:942	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	5	9	theme	composite	971:979	arg1	nanoparticles					981:993	PEI/Chitosan/SiO2 composite nanoparticles	953:993	PEI/Chitosan/SiO2 composite nanoparticles as loading materials	953:1014	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	4	10	theme	sodium	667:672	arg1	molecule					678:685	the fluorescein sodium dye molecule	651:685	the fluorescein sodium dye molecule	651:685	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	5	11	theme	gated	919:923	arg1	sensor					937:942	DNA gated fluorescent sensor	915:942	DNA gated fluorescent sensor	915:942	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	4	12	theme	fluorescein	655:665	arg1	molecule					678:685	the fluorescein sodium dye molecule	651:685	the fluorescein sodium dye molecule	651:685	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	4	13	theme	nanoparticles	750:762	arg1	channels					710:717	the channels	706:717	the channels of PEI/Chitosan/SiO2 composite nanoparticles	706:762	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	5	14	theme	conformation	847:858	arg1	diversity					820:828	the diversity	816:828	the diversity of DNA molecular conformation	816:858	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	5	15	theme	controllable	890:901	arg1	assembly					903:910	a high sensitive controllable assembly	873:910	a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials	873:1014	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	2	16	theme	polymer	274:280	arg1	PEI					282:284	The polymer PEI	270:284	The polymer PEI	270:284	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	3	17	theme	Chitosan	428:435	arg1	effect					418:423	the good pore forming effect	396:423	the good pore forming effect of Chitosan	396:435	And, the good pore forming effect of Chitosan was introduced into SiO2 nanoparticles, and the resulting composite nanoparticles also had a porous structure.
29235238	2	18	theme	amino	314:318	arg1	groups					320:325	amino groups	314:325	amino groups	314:325	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	3	19	theme	forming	410:416	arg1	effect					418:423	the good pore forming effect	396:423	the good pore forming effect of Chitosan	396:435	And, the good pore forming effect of Chitosan was introduced into SiO2 nanoparticles, and the resulting composite nanoparticles also had a porous structure.
29235238	5	20	theme	sensor	937:942	arg1	assembly					903:910	a high sensitive controllable assembly	873:910	a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials	873:1014	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	2	21	theme	SiO2	371:374	arg1	nanoparticles					376:388	the amino functionalized SiO2 nanoparticles	346:388	the amino functionalized SiO2 nanoparticles	346:388	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	0	22	theme	controlled	13:22	arg1	assembly					24:31	the controlled assembly	9:31	the controlled assembly of DNA	9:38	Study of the controlled assembly of DNA gated PEI/Chitosan/SiO2 fluorescent sensor.
29235238	1	23	theme	dioxide	183:189	arg1	nanoparticles					199:211	silicon dioxide (SiO2 ) nanoparticles	175:211	silicon dioxide (SiO2 ) nanoparticles	175:211	In this paper, polyethylenimine (PEI) and Chitosan were simultaneously one-step doped into silicon dioxide (SiO2 ) nanoparticles to synthesize PEI/Chitosan/SiO2 composite nanoparticles.
29235238	2	24	contain	contained	286:294	arg2	groups					320:325	amino groups	314:325	amino groups	314:325	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	2	24	contain	contained	286:294	arg2	amount					304:309	a large amount	296:309	a large amount	296:309	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	2	24	contain	contained	286:294	arg1	PEI					282:284	The polymer PEI	270:284	The polymer PEI	270:284	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	2	25	theme	large	298:302	arg1	amount					304:309	a large amount	296:309	a large amount	296:309	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	2	25	theme	large	298:302	arg1	groups					320:325	amino groups	314:325	amino groups	314:325	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	3	26	theme	SiO2	457:460	arg1	nanoparticles					462:474	SiO2 nanoparticles	457:474	SiO2 nanoparticles	457:474	And, the good pore forming effect of Chitosan was introduced into SiO2 nanoparticles, and the resulting composite nanoparticles also had a porous structure.
29235238	3	27	theme	good	400:403	arg1	effect					418:423	the good pore forming effect	396:423	the good pore forming effect of Chitosan	396:435	And, the good pore forming effect of Chitosan was introduced into SiO2 nanoparticles, and the resulting composite nanoparticles also had a porous structure.
29235238	5	28	theme	PEI/Chitosan/SiO2	953:969	arg1	nanoparticles					981:993	PEI/Chitosan/SiO2 composite nanoparticles	953:993	PEI/Chitosan/SiO2 composite nanoparticles as loading materials	953:1014	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	4	29	contain	had	615:617	arg1	PEI					611:613	PEI	611:613	PEI	611:613	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	4	29	contain	had	615:617	arg1	groups					601:606	the amino groups	591:606	the amino groups of PEI	591:613	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	4	29	contain	had	615:617	arg2	charges					628:634	positive charges	619:634	positive charges	619:634	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	1	30	theme	silicon	175:181	arg1	SiO2					192:195	SiO2	192:195	SiO2	192:195	In this paper, polyethylenimine (PEI) and Chitosan were simultaneously one-step doped into silicon dioxide (SiO2 ) nanoparticles to synthesize PEI/Chitosan/SiO2 composite nanoparticles.
29235238	1	30	theme	silicon	175:181	arg1	dioxide					183:189	silicon dioxide	175:189	silicon dioxide (SiO2 ) nanoparticles	175:211	In this paper, polyethylenimine (PEI) and Chitosan were simultaneously one-step doped into silicon dioxide (SiO2 ) nanoparticles to synthesize PEI/Chitosan/SiO2 composite nanoparticles.
29235238	5	31	theme	DNA	833:835	arg1	conformation					847:858	DNA molecular conformation	833:858	DNA molecular conformation	833:858	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	0	32	theme	assembly	24:31	arg1	Study					0:4	Study	0:4	Study of the controlled assembly of DNA	0:38	Study of the controlled assembly of DNA gated PEI/Chitosan/SiO2 fluorescent sensor.
29235238	3	33	theme	pore	405:408	arg1	effect					418:423	the good pore forming effect	396:423	the good pore forming effect of Chitosan	396:435	And, the good pore forming effect of Chitosan was introduced into SiO2 nanoparticles, and the resulting composite nanoparticles also had a porous structure.
29235238	5	34	theme	molecular	837:845	arg1	conformation					847:858	DNA molecular conformation	833:858	DNA molecular conformation	833:858	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	4	35	theme	PEI	611:613	arg1	PEI					611:613	PEI	611:613	PEI	611:613	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	4	35	theme	PEI	611:613	arg1	groups					601:606	the amino groups	591:606	the amino groups of PEI	591:613	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	3	36	contain	had	524:526	arg2	structure					537:545	a porous structure	528:545	a porous structure	528:545	And, the good pore forming effect of Chitosan was introduced into SiO2 nanoparticles, and the resulting composite nanoparticles also had a porous structure.
29235238	3	36	contain	had	524:526	arg1	nanoparticles					505:517	the resulting composite nanoparticles	481:517	the resulting composite nanoparticles	481:517	And, the good pore forming effect of Chitosan was introduced into SiO2 nanoparticles, and the resulting composite nanoparticles also had a porous structure.
29235238	0	37	theme	DNA	36:38	arg1	assembly					24:31	the controlled assembly	9:31	the controlled assembly of DNA	9:38	Study of the controlled assembly of DNA gated PEI/Chitosan/SiO2 fluorescent sensor.
29235238	4	38	theme	dye	674:676	arg1	molecule					678:685	the fluorescein sodium dye molecule	651:685	the fluorescein sodium dye molecule	651:685	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	5	39	theme	loading	998:1004	arg1	materials					1006:1014	loading materials	998:1014	loading materials	998:1014	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	4	40	theme	electrostatic	767:779	arg1	adsorption					781:790	electrostatic adsorption	767:790	electrostatic adsorption	767:790	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	5	41	theme	DNA	915:917	arg1	sensor					937:942	DNA gated fluorescent sensor	915:942	DNA gated fluorescent sensor	915:942	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	6	42	theme	sensing	1043:1049	arg1	performance					1051:1061	the sensing performance	1039:1061	the sensing performance of the sensor	1039:1075	The factors affecting the sensing performance of the sensor were investigated, and the sensing mechanism was also further studied.
29235238	4	43	theme	composite	740:748	arg1	nanoparticles					750:762	PEI/Chitosan/SiO2 composite nanoparticles	722:762	PEI/Chitosan/SiO2 composite nanoparticles	722:762	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	4	44	theme	PEI/Chitosan/SiO2	722:738	arg1	nanoparticles					750:762	PEI/Chitosan/SiO2 composite nanoparticles	722:762	PEI/Chitosan/SiO2 composite nanoparticles	722:762	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	2	45	theme	groups	320:325	arg1	amount					304:309	a large amount	296:309	a large amount	296:309	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	2	45	theme	groups	320:325	arg1	groups					320:325	amino groups	314:325	amino groups	314:325	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	0	46	theme	fluorescent	64:74	arg1	sensor					76:81	PEI/Chitosan/SiO2 fluorescent sensor	46:81	PEI/Chitosan/SiO2 fluorescent sensor	46:81	Study of the controlled assembly of DNA gated PEI/Chitosan/SiO2 fluorescent sensor.
29235238	5	47	theme	high	875:878	arg1	assembly					903:910	a high sensitive controllable assembly	873:910	a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials	873:1014	Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials.
29235238	6	48	theme	sensor	1070:1075	arg1	performance					1051:1061	the sensing performance	1039:1061	the sensing performance of the sensor	1039:1075	The factors affecting the sensing performance of the sensor were investigated, and the sensing mechanism was also further studied.
29235238	2	49	theme	functionalized	356:369	arg1	nanoparticles					376:388	the amino functionalized SiO2 nanoparticles	346:388	the amino functionalized SiO2 nanoparticles	346:388	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	4	50	theme	positive	619:626	arg1	charges					628:634	positive charges	619:634	positive charges	619:634	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	4	51	theme	amino	595:599	arg1	PEI					611:613	PEI	611:613	PEI	611:613	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	4	51	theme	amino	595:599	arg1	groups					601:606	the amino groups	591:606	the amino groups of PEI	591:613	In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption.
29235238	0	52	theme	PEI/Chitosan/SiO2	46:62	arg1	sensor					76:81	PEI/Chitosan/SiO2 fluorescent sensor	46:81	PEI/Chitosan/SiO2 fluorescent sensor	46:81	Study of the controlled assembly of DNA gated PEI/Chitosan/SiO2 fluorescent sensor.
29235238	2	53	theme	amino	350:354	arg1	nanoparticles					376:388	the amino functionalized SiO2 nanoparticles	346:388	the amino functionalized SiO2 nanoparticles	346:388	The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles.
29235238	1	54	theme	PEI/Chitosan/SiO2	227:243	arg1	nanoparticles					255:267	PEI/Chitosan/SiO2 composite nanoparticles	227:267	PEI/Chitosan/SiO2 composite nanoparticles	227:267	In this paper, polyethylenimine (PEI) and Chitosan were simultaneously one-step doped into silicon dioxide (SiO2 ) nanoparticles to synthesize PEI/Chitosan/SiO2 composite nanoparticles.
25640328	7	0	theme	phenotype	1169:1177	arg1	expression					1192:1201	higher mRNA expression	1180:1201	higher mRNA expression of extracellular matrix and NP specific markers	1180:1249	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	7	0	theme	phenotype	1169:1177	arg1	restoration					1147:1157	improved restoration	1138:1157	improved restoration of the NP phenotype	1138:1177	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	7	0	theme	phenotype	1169:1177	arg1	elaboration					1276:1286	more uniform matrix elaboration	1256:1286	more uniform matrix elaboration	1256:1286	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	7	1	theme	bulk	1045:1048	arg1	mechanics					1050:1058	bulk mechanics	1045:1058	bulk mechanics	1045:1058	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	2	2	theme	long-term	279:287	arg1	efficacy					289:296	poor long-term efficacy	274:296	poor long-term efficacy	274:296	Since current treatments for discogenic low back pain show poor long-term efficacy, a number of new biological strategies are being pursued.
25640328	4	3	from	effects	616:622	arg1	elaboration					685:695	NP cell functional extracellular matrix elaboration	645:695	NP cell functional extracellular matrix elaboration in 3D culture	645:709	Therefore, the objective of this study was to investigate the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture.
25640328	5	4	theme	20	809:810	arg1	%					811:811	%	811:811	%	811:811	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	4	5	theme	oxygen	627:632	arg1	tension					634:640	oxygen tension	627:640	oxygen tension	627:640	Therefore, the objective of this study was to investigate the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture.
25640328	3	6	theme	oxygen	538:543	arg1	tension					545:551	low oxygen tension	534:551	low oxygen tension	534:551	For such therapies to succeed, it is critical that they be validated in conditions that mimic the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension.
25640328	6	7	theme	time	897:900	arg1	point					902:906	each time point	892:906	each time point	892:906	At each time point, extracellular matrix composition, biomechanics, and mRNA expression of key phenotypic markers were evaluated.
25640328	5	8	theme	42	791:792	arg1	days					794:797	14 or 42 days	785:797	days	794:797	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	0	9	from	cells	86:90	arg1	culture					113:119	long-term agarose culture	95:119	long-term agarose culture	95:119	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.
25640328	5	10	from	days	794:797	arg1	oxygen					819:824	either 20% or 2% oxygen	802:824	either 20% or 2% oxygen	802:824	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	5	11	contain	containing	843:852	arg1	media					837:841	defined media	829:841	defined media containing transforming growth factor beta-3	829:886	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	5	11	contain	containing	843:852	arg2	beta-3					881:886	transforming growth factor beta-3	854:886	transforming growth factor beta-3	854:886	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	4	12	theme	extracellular	664:676	arg1	elaboration					685:695	NP cell functional extracellular matrix elaboration	645:695	NP cell functional extracellular matrix elaboration in 3D culture	645:709	Therefore, the objective of this study was to investigate the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture.
25640328	8	13	theme	cell	1420:1423	arg1	strategies					1462:1471	cell and growth factor-based regenerative strategies	1420:1471	cell and growth factor-based regenerative strategies for the disc	1420:1484	These findings indicate that culture under physiological oxygen levels is an important consideration for successful development of cell and growth factor-based regenerative strategies for the disc.
25640328	1	14	theme	intervertebral	142:155	arg1	discs					157:161	the intervertebral discs	138:161	the intervertebral discs	138:161	Degeneration of the intervertebral discs is strongly implicated as a cause of low back pain.
25640328	8	15	theme	growth	1429:1434	arg1	strategies					1462:1471	cell and growth factor-based regenerative strategies	1420:1471	cell and growth factor-based regenerative strategies for the disc	1420:1484	These findings indicate that culture under physiological oxygen levels is an important consideration for successful development of cell and growth factor-based regenerative strategies for the disc.
25640328	7	16	theme	matrix	1269:1274	arg1	elaboration					1276:1286	more uniform matrix elaboration	1256:1286	more uniform matrix elaboration	1256:1286	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	6	17	theme	key	980:982	arg1	markers					995:1001	key phenotypic markers	980:1001	key phenotypic markers	980:1001	At each time point, extracellular matrix composition, biomechanics, and mRNA expression of key phenotypic markers were evaluated.
25640328	7	18	theme	NP	1166:1167	arg1	phenotype					1169:1177	the NP phenotype	1162:1177	the NP phenotype	1162:1177	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	5	19	theme	14	785:786	arg1	days					794:797	14 or 42 days	785:797	days	794:797	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	5	20	theme	%	817:817	arg1	oxygen					819:824	either 20% or 2% oxygen	802:824	either 20% or 2% oxygen	802:824	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	8	21	theme	regenerative	1449:1460	arg1	strategies					1462:1471	cell and growth factor-based regenerative strategies	1420:1471	cell and growth factor-based regenerative strategies for the disc	1420:1484	These findings indicate that culture under physiological oxygen levels is an important consideration for successful development of cell and growth factor-based regenerative strategies for the disc.
25640328	4	22	theme	cell	648:651	arg1	elaboration					685:695	NP cell functional extracellular matrix elaboration	645:695	NP cell functional extracellular matrix elaboration in 3D culture	645:709	Therefore, the objective of this study was to investigate the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture.
25640328	6	23	theme	matrix	923:928	arg1	composition					930:940	extracellular matrix composition	909:940	extracellular matrix composition	909:940	At each time point, extracellular matrix composition, biomechanics, and mRNA expression of key phenotypic markers were evaluated.
25640328	0	24	theme	long-term	95:103	arg1	culture					113:119	long-term agarose culture	95:119	long-term agarose culture	95:119	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.
25640328	5	25	theme	transforming	854:865	arg1	factor					874:879	transforming growth factor	854:879	transforming growth factor beta-3	854:886	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	7	26	dep	matrix	1220:1225	arg1	markers					1243:1249	specific markers	1234:1249	specific markers	1234:1249	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	7	27	theme	uniform	1261:1267	arg1	elaboration					1276:1286	more uniform matrix elaboration	1256:1286	more uniform matrix elaboration	1256:1286	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	0	28	theme	pulposus	77:84	arg1	cells					86:90	nucleus pulposus cells	69:90	nucleus pulposus cells in long-term agarose culture	69:119	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.
25640328	4	29	theme	3D	700:701	arg1	culture					703:709	3D culture	700:709	3D culture	700:709	Therefore, the objective of this study was to investigate the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture.
25640328	0	30	theme	Hypoxic	0:6	arg1	regulation					8:17	Hypoxic regulation	0:17	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.	0:120	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.
25640328	5	31	theme	factor	874:879	arg1	beta-3					881:886	transforming growth factor beta-3	854:886	transforming growth factor beta-3	854:886	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	6	32	theme	phenotypic	984:993	arg1	markers					995:1001	key phenotypic markers	980:1001	key phenotypic markers	980:1001	At each time point, extracellular matrix composition, biomechanics, and mRNA expression of key phenotypic markers were evaluated.
25640328	3	33	theme	such	360:363	arg1	therapies					365:373	such therapies	360:373	such therapies to succeed	360:384	For such therapies to succeed, it is critical that they be validated in conditions that mimic the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension.
25640328	7	34	theme	oxygen	1104:1109	arg1	level					1111:1115	oxygen level	1104:1115	oxygen level	1104:1115	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	1	35	theme	discs	157:161	arg1	cause					191:195	a cause	189:195	a cause of low back pain	189:212	Degeneration of the intervertebral discs is strongly implicated as a cause of low back pain.
25640328	1	35	theme	discs	157:161	arg1	Degeneration					122:133	Degeneration	122:133	Degeneration of the intervertebral discs	122:161	Degeneration of the intervertebral discs is strongly implicated as a cause of low back pain.
25640328	0	36	theme	nucleus	69:75	arg1	cells					86:90	nucleus pulposus cells	69:90	nucleus pulposus cells in long-term agarose culture	69:119	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.
25640328	8	37	theme	physiological	1332:1344	arg1	levels					1353:1358	physiological oxygen levels	1332:1358	physiological oxygen levels	1332:1358	These findings indicate that culture under physiological oxygen levels is an important consideration for successful development of cell and growth factor-based regenerative strategies for the disc.
25640328	0	38	theme	extracellular	33:45	arg1	elaboration					54:64	functional extracellular matrix elaboration	22:64	functional extracellular matrix elaboration	22:64	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.
25640328	6	39	theme	mRNA	961:964	arg1	expression					966:975	mRNA expression	961:975	mRNA expression	961:975	At each time point, extracellular matrix composition, biomechanics, and mRNA expression of key phenotypic markers were evaluated.
25640328	5	40	theme	NP	719:720	arg1	cells					722:726	Bovine NP cells	712:726	Bovine NP cells	712:726	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	3	41	theme	biochemical	461:471	arg1	microenvironment					473:488	the unique biochemical microenvironment	450:488	the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension	450:551	For such therapies to succeed, it is critical that they be validated in conditions that mimic the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension.
25640328	0	42	theme	elaboration	54:64	arg1	regulation					8:17	Hypoxic regulation	0:17	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.	0:120	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.
25640328	7	43	theme	matrix	1220:1225	arg1	expression					1192:1201	higher mRNA expression	1180:1201	higher mRNA expression of extracellular matrix and NP specific markers	1180:1249	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	7	43	theme	matrix	1220:1225	arg1	restoration					1147:1157	improved restoration	1138:1157	improved restoration of the NP phenotype	1138:1177	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	7	43	theme	matrix	1220:1225	arg1	elaboration					1276:1286	more uniform matrix elaboration	1256:1286	more uniform matrix elaboration	1256:1286	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	2	44	theme	discogenic	244:253	arg1	pain					264:267	discogenic low back pain	244:267	discogenic low back pain	244:267	Since current treatments for discogenic low back pain show poor long-term efficacy, a number of new biological strategies are being pursued.
25640328	2	45	theme	biological	315:324	arg1	strategies					326:335	new biological strategies	311:335	new biological strategies	311:335	Since current treatments for discogenic low back pain show poor long-term efficacy, a number of new biological strategies are being pursued.
25640328	7	46	theme	NP	1231:1232	arg1	expression					1192:1201	higher mRNA expression	1180:1201	higher mRNA expression of extracellular matrix and NP specific markers	1180:1249	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	7	46	theme	NP	1231:1232	arg1	restoration					1147:1157	improved restoration	1138:1157	improved restoration of the NP phenotype	1138:1177	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	7	46	theme	NP	1231:1232	arg1	elaboration					1276:1286	more uniform matrix elaboration	1256:1286	more uniform matrix elaboration	1256:1286	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	3	47	theme	nucleus	497:503	arg1	NP					515:516	NP	515:516	NP	515:516	For such therapies to succeed, it is critical that they be validated in conditions that mimic the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension.
25640328	3	47	theme	nucleus	497:503	arg1	pulposus					505:512	the nucleus pulposus	493:512	the nucleus pulposus (NP)	493:517	For such therapies to succeed, it is critical that they be validated in conditions that mimic the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension.
25640328	7	48	theme	mRNA	1187:1190	arg1	expression					1192:1201	higher mRNA expression	1180:1201	higher mRNA expression of extracellular matrix and NP specific markers	1180:1249	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	2	49	theme	poor	274:277	arg1	efficacy					289:296	poor long-term efficacy	274:296	poor long-term efficacy	274:296	Since current treatments for discogenic low back pain show poor long-term efficacy, a number of new biological strategies are being pursued.
25640328	3	50	theme	low	534:536	arg1	tension					545:551	low oxygen tension	534:551	low oxygen tension	534:551	For such therapies to succeed, it is critical that they be validated in conditions that mimic the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension.
25640328	5	51	theme	%	811:811	arg1	oxygen					819:824	either 20% or 2% oxygen	802:824	either 20% or 2% oxygen	802:824	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	1	52	theme	low	200:202	arg1	pain					209:212	low back pain	200:212	low back pain	200:212	Degeneration of the intervertebral discs is strongly implicated as a cause of low back pain.
25640328	8	53	theme	successful	1394:1403	arg1	development					1405:1415	successful development	1394:1415	successful development of cell and growth factor-based regenerative strategies for the disc	1394:1484	These findings indicate that culture under physiological oxygen levels is an important consideration for successful development of cell and growth factor-based regenerative strategies for the disc.
25640328	4	54	theme	study	587:591	arg1	objective					569:577	the objective	565:577	the objective of this study	565:591	Therefore, the objective of this study was to investigate the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture.
25640328	1	55	theme	pain	209:212	arg1	cause					191:195	a cause	189:195	a cause of low back pain	189:212	Degeneration of the intervertebral discs is strongly implicated as a cause of low back pain.
25640328	1	55	theme	pain	209:212	arg1	Degeneration					122:133	Degeneration	122:133	Degeneration of the intervertebral discs	122:161	Degeneration of the intervertebral discs is strongly implicated as a cause of low back pain.
25640328	2	56	theme	current	221:227	arg1	treatments					229:238	current treatments	221:238	current treatments for discogenic low back pain	221:267	Since current treatments for discogenic low back pain show poor long-term efficacy, a number of new biological strategies are being pursued.
25640328	5	57	theme	defined	829:835	arg1	media					837:841	defined media	829:841	defined media containing transforming growth factor beta-3	829:886	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	4	58	theme	functional	653:662	arg1	elaboration					685:695	NP cell functional extracellular matrix elaboration	645:695	NP cell functional extracellular matrix elaboration in 3D culture	645:709	Therefore, the objective of this study was to investigate the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture.
25640328	8	59	theme	factor-based	1436:1447	arg1	strategies					1462:1471	cell and growth factor-based regenerative strategies	1420:1471	cell and growth factor-based regenerative strategies for the disc	1420:1484	These findings indicate that culture under physiological oxygen levels is an important consideration for successful development of cell and growth factor-based regenerative strategies for the disc.
25640328	4	60	theme	matrix	678:683	arg1	elaboration					685:695	NP cell functional extracellular matrix elaboration	645:695	NP cell functional extracellular matrix elaboration in 3D culture	645:709	Therefore, the objective of this study was to investigate the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture.
25640328	5	61	theme	Bovine	712:717	arg1	cells					722:726	Bovine NP cells	712:726	Bovine NP cells	712:726	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	2	62	theme	low	255:257	arg1	pain					264:267	discogenic low back pain	244:267	discogenic low back pain	244:267	Since current treatments for discogenic low back pain show poor long-term efficacy, a number of new biological strategies are being pursued.
25640328	5	63	theme	2	816:816	arg1	%					817:817	%	817:817	%	817:817	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	8	64	theme	strategies	1462:1471	arg1	development					1405:1415	successful development	1394:1415	successful development of cell and growth factor-based regenerative strategies for the disc	1394:1484	These findings indicate that culture under physiological oxygen levels is an important consideration for successful development of cell and growth factor-based regenerative strategies for the disc.
25640328	4	65	theme	tension	634:640	arg1	effects					616:622	the effects	612:622	the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture	612:709	Therefore, the objective of this study was to investigate the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture.
25640328	7	66	theme	improved	1138:1145	arg1	restoration					1147:1157	improved restoration	1138:1157	improved restoration of the NP phenotype	1138:1177	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	3	67	dep	therapies	365:373	arg1	succeed					378:384	succeed	378:384	to succeed	375:384	For such therapies to succeed, it is critical that they be validated in conditions that mimic the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension.
25640328	4	68	theme	NP	645:646	arg1	elaboration					685:695	NP cell functional extracellular matrix elaboration	645:695	NP cell functional extracellular matrix elaboration in 3D culture	645:709	Therefore, the objective of this study was to investigate the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture.
25640328	0	69	theme	agarose	105:111	arg1	culture					113:119	long-term agarose culture	95:119	long-term agarose culture	95:119	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.
25640328	6	70	theme	extracellular	909:921	arg1	composition					930:940	extracellular matrix composition	909:940	extracellular matrix composition	909:940	At each time point, extracellular matrix composition, biomechanics, and mRNA expression of key phenotypic markers were evaluated.
25640328	3	71	theme	unique	454:459	arg1	microenvironment					473:488	the unique biochemical microenvironment	450:488	the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension	450:551	For such therapies to succeed, it is critical that they be validated in conditions that mimic the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension.
25640328	7	72	theme	low	1118:1120	arg1	oxygen					1122:1127	low oxygen	1118:1127	low oxygen	1118:1127	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	7	73	theme	level	1111:1115	arg1	independent					1089:1099	independent	1089:1099	independent	1089:1099	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	5	74	theme	growth	867:872	arg1	factor					874:879	transforming growth factor	854:879	transforming growth factor beta-3	854:886	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	6	75	theme	markers	995:1001	arg1	biomechanics					943:954	biomechanics	943:954	biomechanics	943:954	At each time point, extracellular matrix composition, biomechanics, and mRNA expression of key phenotypic markers were evaluated.
25640328	6	75	theme	markers	995:1001	arg1	composition					930:940	extracellular matrix composition	909:940	extracellular matrix composition	909:940	At each time point, extracellular matrix composition, biomechanics, and mRNA expression of key phenotypic markers were evaluated.
25640328	6	75	theme	markers	995:1001	arg1	expression					966:975	mRNA expression	961:975	mRNA expression	961:975	At each time point, extracellular matrix composition, biomechanics, and mRNA expression of key phenotypic markers were evaluated.
25640328	0	76	theme	functional	22:31	arg1	elaboration					54:64	functional extracellular matrix elaboration	22:64	functional extracellular matrix elaboration	22:64	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.
25640328	7	77	theme	specific	1234:1241	arg1	markers					1243:1249	specific markers	1234:1249	specific markers	1234:1249	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	0	78	theme	matrix	47:52	arg1	elaboration					54:64	functional extracellular matrix elaboration	22:64	functional extracellular matrix elaboration	22:64	Hypoxic regulation of functional extracellular matrix elaboration by nucleus pulposus cells in long-term agarose culture.
25640328	5	79	theme	agarose	749:755	arg1	constructs					757:766	agarose constructs	749:766	agarose constructs	749:766	Bovine NP cells were encapsulated in agarose constructs and cultured for 14 or 42 days in either 20% or 2% oxygen in defined media containing transforming growth factor beta-3.
25640328	7	80	theme	extracellular	1206:1218	arg1	matrix					1220:1225	extracellular matrix	1206:1225	extracellular matrix	1206:1225	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	8	81	theme	oxygen	1346:1351	arg1	levels					1353:1358	physiological oxygen levels	1332:1358	physiological oxygen levels	1332:1358	These findings indicate that culture under physiological oxygen levels is an important consideration for successful development of cell and growth factor-based regenerative strategies for the disc.
25640328	2	82	theme	strategies	326:335	arg1	number					301:306	a number	299:306	a number of new biological strategies	299:335	Since current treatments for discogenic low back pain show poor long-term efficacy, a number of new biological strategies are being pursued.
25640328	4	83	from	elaboration	685:695	arg1	culture					703:709	3D culture	700:709	3D culture	700:709	Therefore, the objective of this study was to investigate the effects of oxygen tension on NP cell functional extracellular matrix elaboration in 3D culture.
25640328	2	84	theme	new	311:313	arg1	strategies					326:335	new biological strategies	311:335	new biological strategies	311:335	Since current treatments for discogenic low back pain show poor long-term efficacy, a number of new biological strategies are being pursued.
25640328	7	85	theme	higher	1180:1185	arg1	expression					1192:1201	higher mRNA expression	1180:1201	higher mRNA expression of extracellular matrix and NP specific markers	1180:1249	Results showed that while bulk mechanics and composition were largely independent of oxygen level, low oxygen promoted improved restoration of the NP phenotype, higher mRNA expression of extracellular matrix and NP specific markers, and more uniform matrix elaboration.
25640328	8	86	theme	important	1366:1374	arg1	culture					1318:1324	culture	1318:1324	culture under physiological oxygen levels	1318:1358	These findings indicate that culture under physiological oxygen levels is an important consideration for successful development of cell and growth factor-based regenerative strategies for the disc.
25640328	8	86	theme	important	1366:1374	arg1	consideration					1376:1388	an important consideration	1363:1388	an important consideration for successful development of cell and growth factor-based regenerative strategies for the disc	1363:1484	These findings indicate that culture under physiological oxygen levels is an important consideration for successful development of cell and growth factor-based regenerative strategies for the disc.
25640328	3	87	theme	pulposus	505:512	arg1	microenvironment					473:488	the unique biochemical microenvironment	450:488	the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension	450:551	For such therapies to succeed, it is critical that they be validated in conditions that mimic the unique biochemical microenvironment of the nucleus pulposus (NP), which include low oxygen tension.
26114646	9	0	theme	pneumonia	1683:1691	arg1	model					1674:1678	a murine model	1665:1678	a murine model of pneumonia	1665:1691	Finally, using a murMN deletion mutant, we observed that increased Ply release is detrimental to virulence during a murine model of pneumonia.
26114646	7	1	theme	surface-associated	1262:1279	arg1	proteins					1297:1304	surface-associated choline-binding proteins	1262:1304	surface-associated choline-binding proteins involved in PG metabolism	1262:1330	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	10	2	theme	branched	1746:1753	arg1	peptides					1760:1767	branched stem peptides	1746:1767	branched stem peptides	1746:1767	Taken together, our results reveal a novel role for branched stem peptides in pneumococcal pathogenesis and demonstrate the importance of controlled Ply release during infection.
26114646	11	3	theme	composition	1918:1928	arg1	importance					1901:1910	the importance	1897:1910	the importance of PG composition in pathogenesis	1897:1944	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	10	4	from	role	1737:1740	arg1	pathogenesis					1785:1796	pneumococcal pathogenesis	1772:1796	pneumococcal pathogenesis	1772:1796	Taken together, our results reveal a novel role for branched stem peptides in pneumococcal pathogenesis and demonstrate the importance of controlled Ply release during infection.
26114646	2	5	dep	multi-functional	261:276	arg1	extracellular					279:291	extracellular	279:291	extracellular	279:291	Pneumolysin (Ply) is a multi-functional, extracellular virulence factor produced by this organism that is critical for pathogenesis.
26114646	2	6	theme	virulence	293:301	arg1	factor					303:308	a multi-functional, extracellular virulence factor	259:308	a multi-functional, extracellular virulence factor produced by this organism that is critical for pathogenesis	259:368	Pneumolysin (Ply) is a multi-functional, extracellular virulence factor produced by this organism that is critical for pathogenesis.
26114646	2	6	theme	virulence	293:301	arg1	Pneumolysin					238:248	Pneumolysin	238:248	Pneumolysin (Ply)	238:254	Pneumolysin (Ply) is a multi-functional, extracellular virulence factor produced by this organism that is critical for pathogenesis.
26114646	6	7	theme	PG	802:803	arg1	dependent					832:840	dependent	832:840	dependent	832:840	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	6	7	theme	PG	802:803	arg1	ability					791:797	The ability	787:797	The ability of PG to inhibit Ply release	787:826	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	1	8	theme	significant	206:216	arg1	worldwide					227:235	a significant pathogen worldwide	204:235	a significant pathogen worldwide	204:235	Streptococcus pneumoniae (the pneumococcus) colonizes the human nasopharynx and is a significant pathogen worldwide.
26114646	7	9	with	mutant	1152:1157	arg1	proportion					1171:1180	a high proportion	1164:1180	a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism	1164:1330	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	5	10	with	assays	606:611	arg1	cells					624:628	whole cells	618:628	whole cells	618:628	Through functional assays with whole cells and subcellular fractions, we determined that Ply activity and its release into the extracellular environment are inhibited by peptidoglycan (PG) structure.
26114646	5	10	with	assays	606:611	arg1	fractions					646:654	subcellular fractions	634:654	subcellular fractions	634:654	Through functional assays with whole cells and subcellular fractions, we determined that Ply activity and its release into the extracellular environment are inhibited by peptidoglycan (PG) structure.
26114646	11	11	theme	other	2020:2024	arg1	pathogens					2036:2044	other bacterial pathogens	2020:2044	other bacterial pathogens	2020:2044	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	8	12	theme	clinical	1336:1343	arg1	relevance					1345:1353	clinical relevance	1336:1353	clinical relevance	1336:1353	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	10	13	theme	Ply	1843:1845	arg1	release					1847:1853	controlled Ply release	1832:1853	controlled Ply release	1832:1853	Taken together, our results reveal a novel role for branched stem peptides in pneumococcal pathogenesis and demonstrate the importance of controlled Ply release during infection.
26114646	6	14	theme	murMN	938:942	arg1	operon					944:949	the murMN operon	934:949	the murMN operon that encodes proteins responsible for branched stem peptide synthesis	934:1019	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	6	14	theme	murMN	938:942	arg1	proteins					964:971	proteins	964:971	proteins responsible for branched stem peptide synthesis	964:1019	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	7	15	theme	proteins	1063:1070	arg1	removal					1036:1042	removal	1036:1042	removal of choline-binding proteins from the cell surface	1036:1092	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	8	16	theme	stem	1536:1539	arg1	peptides					1541:1548	branched stem peptides	1527:1548	branched stem peptides	1527:1548	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	0	17	theme	Stem	23:26	arg1	Peptides					28:35	Peptidoglycan Branched Stem Peptides	0:35	Peptidoglycan Branched Stem Peptides	0:35	Peptidoglycan Branched Stem Peptides Contribute to Streptococcus pneumoniae Virulence by Inhibiting Pneumolysin Release.
26114646	9	18	theme	murMN	1568:1572	arg1	mutant					1583:1588	a murMN deletion mutant	1566:1588	a murMN deletion mutant	1566:1588	Finally, using a murMN deletion mutant, we observed that increased Ply release is detrimental to virulence during a murine model of pneumonia.
26114646	5	19	theme	extracellular	714:726	arg1	environment					728:738	the extracellular environment	710:738	the extracellular environment	710:738	Through functional assays with whole cells and subcellular fractions, we determined that Ply activity and its release into the extracellular environment are inhibited by peptidoglycan (PG) structure.
26114646	7	20	theme	structural	1239:1248	arg1	feature					1250:1256	this structural feature	1234:1256	this structural feature	1234:1256	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	11	21	theme	bacterial	2026:2034	arg1	pathogens					2036:2044	other bacterial pathogens	2020:2044	other bacterial pathogens	2020:2044	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	8	22	theme	hyperactive	1383:1393	arg1	allele					1409:1414	a hyperactive, mosaic murMN allele	1381:1414	a hyperactive, mosaic murMN allele associated with penicillin resistance	1381:1452	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	7	23	located	observed	1138:1145	arg2	levels					1131:1136	levels	1131:1136	levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism	1131:1330	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	7	23	located	observed	1138:1145	arg1	mutant					1152:1157	a mutant	1150:1157	a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism	1150:1330	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	5	24	theme	subcellular	634:644	arg1	fractions					646:654	subcellular fractions	634:654	subcellular fractions	634:654	Through functional assays with whole cells and subcellular fractions, we determined that Ply activity and its release into the extracellular environment are inhibited by peptidoglycan (PG) structure.
26114646	6	25	theme	stem	998:1001	arg1	synthesis					1011:1019	branched stem peptide synthesis	989:1019	branched stem peptide synthesis	989:1019	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	11	26	theme	broad	1959:1963	arg1	implications					1965:1976	broad implications	1959:1976	broad implications for the diverse PG structures observed in other bacterial pathogens	1959:2044	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	5	27	theme	functional	595:604	arg1	assays					606:611	functional assays	595:611	functional assays with whole cells and subcellular fractions	595:654	Through functional assays with whole cells and subcellular fractions, we determined that Ply activity and its release into the extracellular environment are inhibited by peptidoglycan (PG) structure.
26114646	3	28	theme	secretion	407:415	arg1	absence					383:389	the absence	379:389	the absence of any apparent secretion or cell surface attachment motifs	379:449	Despite the absence of any apparent secretion or cell surface attachment motifs, Ply localizes to the cell envelope of actively growing cells.
26114646	11	29	from	importance	1901:1910	arg1	pathogenesis					1933:1944	pathogenesis	1933:1944	pathogenesis	1933:1944	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	0	30	theme	Peptidoglycan	0:12	arg1	Peptides					28:35	Peptidoglycan Branched Stem Peptides	0:35	Peptidoglycan Branched Stem Peptides	0:35	Peptidoglycan Branched Stem Peptides Contribute to Streptococcus pneumoniae Virulence by Inhibiting Pneumolysin Release.
26114646	9	31	theme	Ply	1618:1620	arg1	release					1622:1628	increased Ply release	1608:1628	increased Ply release	1608:1628	Finally, using a murMN deletion mutant, we observed that increased Ply release is detrimental to virulence during a murine model of pneumonia.
26114646	4	32	theme	surface	565:571	arg1	localization					573:584	this surface localization	560:584	this surface localization	560:584	We sought to characterize the consequences of this surface localization.
26114646	11	33	theme	diverse	1986:1992	arg1	structures					1997:2006	the diverse PG structures	1982:2006	the diverse PG structures observed in other bacterial pathogens	1982:2044	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	7	34	theme	branched	1185:1192	arg1	peptides					1199:1206	branched stem peptides	1185:1206	branched stem peptides suggesting a link between this structural feature	1185:1256	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	6	35	theme	macromolecule	882:894	arg1	composition					862:872	the stem peptide composition	845:872	the stem peptide composition	845:872	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	3	36	theme	attachment	433:442	arg1	motifs					444:449	cell surface attachment motifs	420:449	cell surface attachment motifs	420:449	Despite the absence of any apparent secretion or cell surface attachment motifs, Ply localizes to the cell envelope of actively growing cells.
26114646	7	37	theme	peptides	1199:1206	arg1	proportion					1171:1180	a high proportion	1164:1180	a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism	1164:1330	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	0	38	dep	Streptococcus	51:63	arg1	pneumoniae					65:74	pneumoniae	65:74	pneumoniae	65:74	Peptidoglycan Branched Stem Peptides Contribute to Streptococcus pneumoniae Virulence by Inhibiting Pneumolysin Release.
26114646	3	39	theme	cell	420:423	arg1	motifs					444:449	cell surface attachment motifs	420:449	cell surface attachment motifs	420:449	Despite the absence of any apparent secretion or cell surface attachment motifs, Ply localizes to the cell envelope of actively growing cells.
26114646	8	40	theme	decreased	1461:1469	arg1	release					1475:1481	decreased Ply release	1461:1481	decreased Ply release	1461:1481	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	3	41	theme	cells	507:511	arg1	envelope					478:485	the cell envelope	469:485	the cell envelope of actively growing cells	469:511	Despite the absence of any apparent secretion or cell surface attachment motifs, Ply localizes to the cell envelope of actively growing cells.
26114646	0	42	theme	Streptococcus	51:63	arg1	Virulence					76:84	Streptococcus pneumoniae Virulence	51:84	Streptococcus pneumoniae Virulence	51:84	Peptidoglycan Branched Stem Peptides Contribute to Streptococcus pneumoniae Virulence by Inhibiting Pneumolysin Release.
26114646	6	43	theme	peptide	854:860	arg1	composition					862:872	the stem peptide composition	845:872	the stem peptide composition	845:872	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	10	44	theme	novel	1731:1735	arg1	role					1737:1740	a novel role	1729:1740	a novel role for branched stem peptides in pneumococcal pathogenesis	1729:1796	Taken together, our results reveal a novel role for branched stem peptides in pneumococcal pathogenesis and demonstrate the importance of controlled Ply release during infection.
26114646	10	45	theme	pneumococcal	1772:1783	arg1	pathogenesis					1785:1796	pneumococcal pathogenesis	1772:1796	pneumococcal pathogenesis	1772:1796	Taken together, our results reveal a novel role for branched stem peptides in pneumococcal pathogenesis and demonstrate the importance of controlled Ply release during infection.
26114646	9	46	theme	murine	1667:1672	arg1	model					1674:1678	a murine model	1665:1678	a murine model of pneumonia	1665:1691	Finally, using a murMN deletion mutant, we observed that increased Ply release is detrimental to virulence during a murine model of pneumonia.
26114646	7	47	theme	PG	1318:1319	arg1	metabolism					1321:1330	PG metabolism	1318:1330	PG metabolism	1318:1330	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	8	48	theme	concomitant	1488:1498	arg1	increases					1500:1508	concomitant increases	1488:1508	concomitant increases in the amount of branched stem peptides	1488:1548	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	5	49	theme	Ply	676:678	arg1	activity					680:687	Ply activity	676:687	Ply activity	676:687	Through functional assays with whole cells and subcellular fractions, we determined that Ply activity and its release into the extracellular environment are inhibited by peptidoglycan (PG) structure.
26114646	10	50	theme	stem	1755:1758	arg1	peptides					1760:1767	branched stem peptides	1746:1767	branched stem peptides	1746:1767	Taken together, our results reveal a novel role for branched stem peptides in pneumococcal pathogenesis and demonstrate the importance of controlled Ply release during infection.
26114646	7	51	theme	choline-binding	1281:1295	arg1	proteins					1297:1304	surface-associated choline-binding proteins	1262:1304	surface-associated choline-binding proteins involved in PG metabolism	1262:1330	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	7	52	from	surface	1086:1092	arg1	removal					1036:1042	removal	1036:1042	removal of choline-binding proteins from the cell surface	1036:1092	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	9	53	theme	deletion	1574:1581	arg1	mutant					1583:1588	a murMN deletion mutant	1566:1588	a murMN deletion mutant	1566:1588	Finally, using a murMN deletion mutant, we observed that increased Ply release is detrimental to virulence during a murine model of pneumonia.
26114646	11	54	located	observed	2008:2015	arg1	pathogens					2036:2044	other bacterial pathogens	2020:2044	other bacterial pathogens	2020:2044	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	11	54	located	observed	2008:2015	arg2	structures					1997:2006	the diverse PG structures	1982:2006	the diverse PG structures observed in other bacterial pathogens	1982:2044	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	6	55	theme	Ply	816:818	arg1	release					820:826	Ply release	816:826	Ply release	816:826	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	8	56	from	increases	1500:1508	arg1	amount					1517:1522	the amount	1513:1522	the amount of branched stem peptides	1513:1548	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	8	56	from	increases	1500:1508	arg1	peptides					1541:1548	branched stem peptides	1527:1548	branched stem peptides	1527:1548	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	7	57	theme	Ply	1116:1118	arg1	release					1120:1126	Ply release	1116:1126	Ply release	1116:1126	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	10	58	theme	controlled	1832:1841	arg1	release					1847:1853	controlled Ply release	1832:1853	controlled Ply release	1832:1853	Taken together, our results reveal a novel role for branched stem peptides in pneumococcal pathogenesis and demonstrate the importance of controlled Ply release during infection.
26114646	6	59	theme	branched	989:996	arg1	synthesis					1011:1019	branched stem peptide synthesis	989:1019	branched stem peptide synthesis	989:1019	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	1	60	theme	pathogen	218:225	arg1	worldwide					227:235	a significant pathogen worldwide	204:235	a significant pathogen worldwide	204:235	Streptococcus pneumoniae (the pneumococcus) colonizes the human nasopharynx and is a significant pathogen worldwide.
26114646	6	61	theme	responsible	973:983	arg1	operon					944:949	the murMN operon	934:949	the murMN operon that encodes proteins responsible for branched stem peptide synthesis	934:1019	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	6	61	theme	responsible	973:983	arg1	proteins					964:971	proteins	964:971	proteins responsible for branched stem peptide synthesis	964:1019	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	7	62	theme	cell	1081:1084	arg1	surface					1086:1092	the cell surface	1077:1092	the cell surface	1077:1092	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	8	63	dep	hyperactive	1383:1393	arg1	mosaic					1396:1401	mosaic	1396:1401	mosaic	1396:1401	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	10	64	theme	release	1847:1853	arg1	importance					1818:1827	the importance	1814:1827	the importance of controlled Ply release during infection	1814:1870	Taken together, our results reveal a novel role for branched stem peptides in pneumococcal pathogenesis and demonstrate the importance of controlled Ply release during infection.
26114646	6	65	theme	operon	944:949	arg1	mutation					922:929	mutation	922:929	mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis	922:1019	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	8	66	theme	branched	1527:1534	arg1	peptides					1541:1548	branched stem peptides	1527:1548	branched stem peptides	1527:1548	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	7	67	theme	choline-binding	1047:1061	arg1	proteins					1063:1070	choline-binding proteins	1047:1070	choline-binding proteins	1047:1070	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	8	68	theme	peptides	1541:1548	arg1	amount					1517:1522	the amount	1513:1522	the amount of branched stem peptides	1513:1548	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	8	68	theme	peptides	1541:1548	arg1	peptides					1541:1548	branched stem peptides	1527:1548	branched stem peptides	1527:1548	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	2	69	theme	multi-functional	261:276	arg1	factor					303:308	a multi-functional, extracellular virulence factor	259:308	a multi-functional, extracellular virulence factor produced by this organism that is critical for pathogenesis	259:368	Pneumolysin (Ply) is a multi-functional, extracellular virulence factor produced by this organism that is critical for pathogenesis.
26114646	2	69	theme	multi-functional	261:276	arg1	Pneumolysin					238:248	Pneumolysin	238:248	Pneumolysin (Ply)	238:254	Pneumolysin (Ply) is a multi-functional, extracellular virulence factor produced by this organism that is critical for pathogenesis.
26114646	0	70	theme	Pneumolysin	100:110	arg1	Release					112:118	Pneumolysin Release	100:118	Pneumolysin Release	100:118	Peptidoglycan Branched Stem Peptides Contribute to Streptococcus pneumoniae Virulence by Inhibiting Pneumolysin Release.
26114646	1	71	theme	human	179:183	arg1	nasopharynx					185:195	the human nasopharynx	175:195	the human nasopharynx	175:195	Streptococcus pneumoniae (the pneumococcus) colonizes the human nasopharynx and is a significant pathogen worldwide.
26114646	5	72	theme	peptidoglycan	757:769	arg1	structure					776:784	peptidoglycan (PG) structure	757:784	peptidoglycan (PG) structure	757:784	Through functional assays with whole cells and subcellular fractions, we determined that Ply activity and its release into the extracellular environment are inhibited by peptidoglycan (PG) structure.
26114646	6	73	theme	peptide	1003:1009	arg1	synthesis					1011:1019	branched stem peptide synthesis	989:1019	branched stem peptide synthesis	989:1019	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	5	74	theme	PG	772:773	arg1	structure					776:784	peptidoglycan (PG) structure	757:784	peptidoglycan (PG) structure	757:784	Through functional assays with whole cells and subcellular fractions, we determined that Ply activity and its release into the extracellular environment are inhibited by peptidoglycan (PG) structure.
26114646	11	75	contain	have	1954:1957	arg2	implications					1965:1976	broad implications	1959:1976	broad implications for the diverse PG structures observed in other bacterial pathogens	1959:2044	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	11	75	contain	have	1954:1957	arg1	results					1879:1885	These results	1873:1885	These results	1873:1885	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	0	76	theme	Branched	14:21	arg1	Peptides					28:35	Peptidoglycan Branched Stem Peptides	0:35	Peptidoglycan Branched Stem Peptides	0:35	Peptidoglycan Branched Stem Peptides Contribute to Streptococcus pneumoniae Virulence by Inhibiting Pneumolysin Release.
26114646	9	77	theme	increased	1608:1616	arg1	release					1622:1628	increased Ply release	1608:1628	increased Ply release	1608:1628	Finally, using a murMN deletion mutant, we observed that increased Ply release is detrimental to virulence during a murine model of pneumonia.
26114646	4	78	theme	localization	573:584	arg1	consequences					544:555	the consequences	540:555	the consequences of this surface localization	540:584	We sought to characterize the consequences of this surface localization.
26114646	8	79	theme	murMN	1403:1407	arg1	allele					1409:1414	a hyperactive, mosaic murMN allele	1381:1414	a hyperactive, mosaic murMN allele associated with penicillin resistance	1381:1452	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	5	80	theme	whole	618:622	arg1	cells					624:628	whole cells	618:628	whole cells	618:628	Through functional assays with whole cells and subcellular fractions, we determined that Ply activity and its release into the extracellular environment are inhibited by peptidoglycan (PG) structure.
26114646	1	81	theme	Streptococcus	121:133	arg1	pneumococcus					151:162	the pneumococcus	147:162	the pneumococcus	147:162	Streptococcus pneumoniae (the pneumococcus) colonizes the human nasopharynx and is a significant pathogen worldwide.
26114646	1	81	theme	Streptococcus	121:133	arg1	pneumoniae					135:144	Streptococcus pneumoniae	121:144	Streptococcus pneumoniae (the pneumococcus)	121:163	Streptococcus pneumoniae (the pneumococcus) colonizes the human nasopharynx and is a significant pathogen worldwide.
26114646	3	82	theme	apparent	398:405	arg1	secretion					407:415	any apparent secretion	394:415	any apparent secretion	394:415	Despite the absence of any apparent secretion or cell surface attachment motifs, Ply localizes to the cell envelope of actively growing cells.
26114646	11	83	theme	PG	1994:1995	arg1	structures					1997:2006	the diverse PG structures	1982:2006	the diverse PG structures observed in other bacterial pathogens	1982:2044	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	7	84	theme	high	1166:1169	arg1	proportion					1171:1180	a high proportion	1164:1180	a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism	1164:1330	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	3	85	theme	motifs	444:449	arg1	absence					383:389	the absence	379:389	the absence of any apparent secretion or cell surface attachment motifs	379:449	Despite the absence of any apparent secretion or cell surface attachment motifs, Ply localizes to the cell envelope of actively growing cells.
26114646	8	86	theme	penicillin	1432:1441	arg1	resistance					1443:1452	penicillin resistance	1432:1452	penicillin resistance	1432:1452	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	3	87	theme	cell	473:476	arg1	envelope					478:485	the cell envelope	469:485	the cell envelope of actively growing cells	469:511	Despite the absence of any apparent secretion or cell surface attachment motifs, Ply localizes to the cell envelope of actively growing cells.
26114646	3	88	theme	surface	425:431	arg1	motifs					444:449	cell surface attachment motifs	420:449	cell surface attachment motifs	420:449	Despite the absence of any apparent secretion or cell surface attachment motifs, Ply localizes to the cell envelope of actively growing cells.
26114646	11	89	theme	PG	1915:1916	arg1	composition					1918:1928	PG composition	1915:1928	PG composition	1915:1928	These results highlight the importance of PG composition in pathogenesis and may have broad implications for the diverse PG structures observed in other bacterial pathogens.
26114646	6	90	theme	stem	849:852	arg1	composition					862:872	the stem peptide composition	845:872	the stem peptide composition	845:872	The ability of PG to inhibit Ply release was dependent on the stem peptide composition of this macromolecule, which was manipulated by mutation of the murMN operon that encodes proteins responsible for branched stem peptide synthesis.
26114646	8	91	theme	Ply	1471:1473	arg1	release					1475:1481	decreased Ply release	1461:1481	decreased Ply release	1461:1481	Of clinical relevance, we also demonstrate that a hyperactive, mosaic murMN allele associated with penicillin resistance causes decreased Ply release with concomitant increases in the amount of branched stem peptides.
26114646	3	92	theme	growing	499:505	arg1	cells					507:511	actively growing cells	490:511	actively growing cells	490:511	Despite the absence of any apparent secretion or cell surface attachment motifs, Ply localizes to the cell envelope of actively growing cells.
26114646	7	93	theme	proteins	1297:1304	arg1	proportion					1171:1180	a high proportion	1164:1180	a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism	1164:1330	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
26114646	7	94	theme	stem	1194:1197	arg1	peptides					1199:1206	branched stem peptides	1185:1206	branched stem peptides suggesting a link between this structural feature	1185:1256	Additionally, removal of choline-binding proteins from the cell surface significantly reduced Ply release to levels observed in a mutant with a high proportion of branched stem peptides suggesting a link between this structural feature and surface-associated choline-binding proteins involved in PG metabolism.
27998147	7	0	theme	erythrocyte	1094:1104	arg1	absorption					1106:1115	erythrocyte absorption	1094:1115	erythrocyte absorption	1094:1115	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	8	1	theme	0.11	1377:1380	arg1	g					1382:1382	0.31 ± 0.11 g	1370:1382	0.31 ± 0.11 g	1370:1382	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	8	1	theme	0.11	1377:1380	arg1	loss					1364:1367	lowest blood loss	1351:1367	lowest blood loss (0.31 ± 0.11 g)	1351:1383	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	7	2	theme	blood	992:996	arg1	study					1020:1024	Further blood coagulation mechanism study	984:1024	Further blood coagulation mechanism study	984:1024	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	6	3	from	mg/mL	976:980	arg1	s					943:943	351 ± 14.73 s	931:943	351 ± 14.73 s at 5 mg/mL	931:954	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	6	3	from	mg/mL	976:980	arg1	32.42s					963:968	248 ± 32.42s	957:968	248 ± 32.42s at 10 mg/mL	957:980	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	8	4	theme	amputation	1397:1406	arg1	model					1408:1412	rat-tail amputation model	1388:1412	rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite	1388:1493	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	6	5	dep	effect	923:928	arg1	s					943:943	351 ± 14.73 s	931:943	351 ± 14.73 s at 5 mg/mL	931:954	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	6	5	dep	effect	923:928	arg1	32.42s					963:968	248 ± 32.42s	957:968	248 ± 32.42s at 10 mg/mL	957:980	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	7	6	theme	coagulation	998:1008	arg1	study					1020:1024	Further blood coagulation mechanism study	984:1024	Further blood coagulation mechanism study	984:1024	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	4	7	theme	hemostatic	403:412	arg1	performance					414:424	the biocompatibility and hemostatic performance	378:424	the biocompatibility and hemostatic performance of diatom silica	378:441	To improve the biocompatibility and hemostatic performance of diatom silica, a series of chitosan-coated diatom (CS-diatom) was developed.
27998147	2	8	theme	Current	161:167	arg1	agents					180:185	Current hemostatic agents	161:185	Current hemostatic agents	161:185	Current hemostatic agents still have various limitations and side effects.
27998147	6	9	theme	hemostasis	912:921	arg1	effect					923:928	desirable hemostasis effect	902:928	desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL)	902:981	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	9	10	theme	hemorrhage	1653:1662	arg1	control					1664:1670	hemorrhage control	1653:1670	hemorrhage control	1653:1670	The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
27998147	6	11	theme	hemolysis	783:791	arg1	%					802:802	hemolysis ratio < 5%	783:802	hemolysis ratio < 5%	783:802	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	8	12	theme	multiple	1251:1258	arg1	performances					1271:1282	the multiple hemostatic performances	1247:1282	the multiple hemostatic performances	1247:1282	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	6	13	theme	desirable	902:910	arg1	effect					923:928	desirable hemostasis effect	902:928	desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL)	902:981	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	9	14	theme	safe	1543:1546	arg1	CS-diatom					1527:1535	the CS-diatom	1523:1535	the CS-diatom	1523:1535	The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
27998147	9	14	theme	safe	1543:1546	arg1	agent					1573:1577	a safe and effective hemostatic agent	1541:1577	a safe and effective hemostatic agent	1541:1577	The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
27998147	6	15	theme	fluid	837:841	arg1	absorbility					843:853	great fluid absorbility	831:853	great fluid absorbility (24.39 ± 1.53 times the weight of liquid)	831:895	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	7	16	theme	Further	984:990	arg1	study					1020:1024	Further blood coagulation mechanism study	984:1024	Further blood coagulation mechanism study	984:1024	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	1	17	dep	military	131:138	arg1	trauma					153:158	trauma	153:158	trauma	153:158	Uncontrolled hemorrhage leads to high death risk both in military and civilian trauma.
27998147	5	18	dep	in	561:562	arg1	vitro					564:568	vitro	564:568	vitro	564:568	The composition of CS-diatom prepared was optimized by in vitro hemocompatibility and blood coagulation evaluation for that prepared with 0.5%, 1%, 3%, and 5% chitosan.
27998147	7	19	theme	intrinsic	1160:1168	arg1	pathway					1182:1188	the intrinsic coagulation pathway	1156:1188	the intrinsic coagulation pathway	1156:1188	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	7	20	theme	ideal	1068:1072	arg1	interface					1074:1082	an ideal interface	1065:1082	an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation	1065:1227	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	6	21	theme	great	831:835	arg1	absorbility					843:853	great fluid absorbility	831:853	great fluid absorbility (24.39 ± 1.53 times the weight of liquid)	831:895	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	1	22	theme	Uncontrolled	74:85	arg1	hemorrhage					87:96	Uncontrolled hemorrhage	74:96	Uncontrolled hemorrhage	74:96	Uncontrolled hemorrhage leads to high death risk both in military and civilian trauma.
27998147	3	23	theme	hemorrhage	347:356	arg1	control					358:364	hemorrhage control	347:364	hemorrhage control	347:364	In this study, natural diatom silica obtained from diatomite and diatom culture was purified and developed for hemorrhage control.
27998147	3	24	theme	natural	251:257	arg1	silica					266:271	natural diatom silica	251:271	natural diatom silica obtained from diatomite and diatom culture	251:314	In this study, natural diatom silica obtained from diatomite and diatom culture was purified and developed for hemorrhage control.
27998147	6	25	theme	favorable	755:763	arg1	biocompatibility					765:780	favorable biocompatibility	755:780	favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs)	755:828	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	8	26	theme	hemostatic	1260:1269	arg1	performances					1271:1282	the multiple hemostatic performances	1247:1282	the multiple hemostatic performances	1247:1282	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	6	27	theme	±	961:961	arg1	s					943:943	351 ± 14.73 s	931:943	351 ± 14.73 s at 5 mg/mL	931:954	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	6	27	theme	±	961:961	arg1	32.42s					963:968	248 ± 32.42s	957:968	248 ± 32.42s at 10 mg/mL	957:980	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	0	28	theme	Diatom	16:21	arg1	Silica					23:28	Diatom Silica	16:28	Diatom Silica	16:28	Chitosan-Coated Diatom Silica as Hemostatic Agent for Hemorrhage Control.
27998147	9	29	theme	nonsynthetic	1615:1626	arg1	materials					1639:1647	nonsynthetic mesoporous materials	1615:1647	nonsynthetic mesoporous materials	1615:1647	The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
27998147	9	30	theme	new	1594:1596	arg1	understanding					1598:1610	a new understanding	1592:1610	a new understanding of nonsynthetic mesoporous materials	1592:1647	The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
27998147	8	31	theme	rat-tail	1388:1395	arg1	model					1408:1412	rat-tail amputation model	1388:1412	rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite	1388:1493	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	9	32	theme	effective	1552:1560	arg1	CS-diatom					1527:1535	the CS-diatom	1523:1535	the CS-diatom	1523:1535	The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
27998147	9	32	theme	effective	1552:1560	arg1	agent					1573:1577	a safe and effective hemostatic agent	1541:1577	a safe and effective hemostatic agent	1541:1577	The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
27998147	4	33	theme	biocompatibility	382:397	arg1	performance					414:424	the biocompatibility and hemostatic performance	378:424	the biocompatibility and hemostatic performance of diatom silica	378:441	To improve the biocompatibility and hemostatic performance of diatom silica, a series of chitosan-coated diatom (CS-diatom) was developed.
27998147	8	34	theme	±	1375:1375	arg1	g					1382:1382	0.31 ± 0.11 g	1370:1382	0.31 ± 0.11 g	1370:1382	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	8	34	theme	±	1375:1375	arg1	loss					1364:1367	lowest blood loss	1351:1367	lowest blood loss (0.31 ± 0.11 g)	1351:1383	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	5	35	theme	blood	592:596	arg1	evaluation					610:619	blood coagulation evaluation	592:619	blood coagulation evaluation	592:619	The composition of CS-diatom prepared was optimized by in vitro hemocompatibility and blood coagulation evaluation for that prepared with 0.5%, 1%, 3%, and 5% chitosan.
27998147	8	36	theme	lowest	1351:1356	arg1	g					1382:1382	0.31 ± 0.11 g	1370:1382	0.31 ± 0.11 g	1370:1382	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	8	36	theme	lowest	1351:1356	arg1	loss					1364:1367	lowest blood loss	1351:1367	lowest blood loss (0.31 ± 0.11 g)	1351:1383	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	5	37	theme	coagulation	598:608	arg1	evaluation					610:619	blood coagulation evaluation	592:619	blood coagulation evaluation	592:619	The composition of CS-diatom prepared was optimized by in vitro hemocompatibility and blood coagulation evaluation for that prepared with 0.5%, 1%, 3%, and 5% chitosan.
27998147	1	38	theme	high	107:110	arg1	risk					118:121	high death risk	107:121	high death risk	107:121	Uncontrolled hemorrhage leads to high death risk both in military and civilian trauma.
27998147	6	39	theme	ratio	793:797	arg1	%					802:802	hemolysis ratio < 5%	783:802	hemolysis ratio < 5%	783:802	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	2	40	contain	have	193:196	arg1	agents					180:185	Current hemostatic agents	161:185	Current hemostatic agents	161:185	Current hemostatic agents still have various limitations and side effects.
27998147	2	40	contain	have	193:196	arg2	limitations					206:216	various limitations	198:216	various limitations	198:216	Current hemostatic agents still have various limitations and side effects.
27998147	2	40	contain	have	193:196	arg2	effects					227:233	side effects	222:233	side effects	222:233	Current hemostatic agents still have various limitations and side effects.
27998147	6	41	theme	liquid	889:894	arg1	weight					879:884	the weight	875:884	24.39 ± 1.53 times the weight of liquid	856:894	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	1	42	dep	risk	118:121	arg1	both					123:126	both	123:126	both	123:126	Uncontrolled hemorrhage leads to high death risk both in military and civilian trauma.
27998147	6	43	dep	times	869:873	arg1	weight					879:884	the weight	875:884	24.39 ± 1.53 times the weight of liquid	856:894	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	1	44	theme	death	112:116	arg1	risk					118:121	high death risk	107:121	high death risk	107:121	Uncontrolled hemorrhage leads to high death risk both in military and civilian trauma.
27998147	7	45	theme	blood	1211:1215	arg1	coagulation					1217:1227	accelerated blood coagulation	1199:1227	accelerated blood coagulation	1199:1227	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	5	46	dep	%	647:647	arg1	chitosan					665:672	chitosan	665:672	chitosan	665:672	The composition of CS-diatom prepared was optimized by in vitro hemocompatibility and blood coagulation evaluation for that prepared with 0.5%, 1%, 3%, and 5% chitosan.
27998147	6	47	dep	%	802:802	arg1	cytotoxicity					808:819	no cytotoxicity	805:819	no cytotoxicity to MEFs	805:827	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	3	48	theme	diatom	259:264	arg1	silica					266:271	natural diatom silica	251:271	natural diatom silica obtained from diatomite and diatom culture	251:314	In this study, natural diatom silica obtained from diatomite and diatom culture was purified and developed for hemorrhage control.
27998147	8	49	theme	clotting	1315:1322	arg1	time					1324:1327	the shortest clotting time	1302:1327	the shortest clotting time (98.34 ± 26.54 s)	1302:1345	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	8	49	theme	clotting	1315:1322	arg1	s					1344:1344	98.34 ± 26.54 s	1330:1344	98.34 ± 26.54 s	1330:1344	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	0	50	theme	Hemostatic	33:42	arg1	Agent					44:48	Hemostatic Agent	33:48	Hemostatic Agent for Hemorrhage Control	33:71	Chitosan-Coated Diatom Silica as Hemostatic Agent for Hemorrhage Control.
27998147	6	51	dep	absorbility	843:853	arg1	times					869:873	24.39 ± 1.53 times	856:873	24.39 ± 1.53 times the weight of liquid	856:894	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	4	52	theme	diatom	472:477	arg1	series					446:451	a series	444:451	a series of chitosan-coated diatom (CS-diatom)	444:489	To improve the biocompatibility and hemostatic performance of diatom silica, a series of chitosan-coated diatom (CS-diatom) was developed.
27998147	8	53	theme	shortest	1306:1313	arg1	time					1324:1327	the shortest clotting time	1302:1327	the shortest clotting time (98.34 ± 26.54 s)	1302:1345	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	8	53	theme	shortest	1306:1313	arg1	s					1344:1344	98.34 ± 26.54 s	1330:1344	98.34 ± 26.54 s	1330:1344	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	6	54	theme	1	733:733	arg1	%					734:734	%	734:734	%	734:734	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	0	55	theme	Hemorrhage	54:63	arg1	Control					65:71	Hemorrhage Control	54:71	Hemorrhage Control	54:71	Chitosan-Coated Diatom Silica as Hemostatic Agent for Hemorrhage Control.
27998147	3	56	theme	diatom	301:306	arg1	culture					308:314	diatom culture	301:314	diatom culture	301:314	In this study, natural diatom silica obtained from diatomite and diatom culture was purified and developed for hemorrhage control.
27998147	4	57	theme	chitosan-coated	456:470	arg1	CS-diatom					480:488	CS-diatom	480:488	CS-diatom	480:488	To improve the biocompatibility and hemostatic performance of diatom silica, a series of chitosan-coated diatom (CS-diatom) was developed.
27998147	4	57	theme	chitosan-coated	456:470	arg1	diatom					472:477	chitosan-coated diatom	456:477	chitosan-coated diatom (CS-diatom)	456:489	To improve the biocompatibility and hemostatic performance of diatom silica, a series of chitosan-coated diatom (CS-diatom) was developed.
27998147	7	58	theme	coagulation	1170:1180	arg1	pathway					1182:1188	the intrinsic coagulation pathway	1156:1188	the intrinsic coagulation pathway	1156:1188	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	9	59	theme	hemostatic	1562:1571	arg1	CS-diatom					1527:1535	the CS-diatom	1523:1535	the CS-diatom	1523:1535	The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
27998147	9	59	theme	hemostatic	1562:1571	arg1	agent					1573:1577	a safe and effective hemostatic agent	1541:1577	a safe and effective hemostatic agent	1541:1577	The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
27998147	4	60	theme	silica	436:441	arg1	performance					414:424	the biocompatibility and hemostatic performance	378:424	the biocompatibility and hemostatic performance of diatom silica	378:441	To improve the biocompatibility and hemostatic performance of diatom silica, a series of chitosan-coated diatom (CS-diatom) was developed.
27998147	2	61	theme	side	222:225	arg1	effects					227:233	side effects	222:233	side effects	222:233	Current hemostatic agents still have various limitations and side effects.
27998147	5	62	theme	in	561:562	arg1	hemocompatibility					570:586	in vitro hemocompatibility	561:586	in vitro hemocompatibility	561:586	The composition of CS-diatom prepared was optimized by in vitro hemocompatibility and blood coagulation evaluation for that prepared with 0.5%, 1%, 3%, and 5% chitosan.
27998147	2	63	theme	hemostatic	169:178	arg1	agents					180:185	Current hemostatic agents	161:185	Current hemostatic agents	161:185	Current hemostatic agents still have various limitations and side effects.
27998147	6	64	theme	%	734:734	arg1	chitosan					736:743	1% chitosan	733:743	1% chitosan	733:743	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	4	65	theme	diatom	429:434	arg1	silica					436:441	diatom silica	429:441	diatom silica	429:441	To improve the biocompatibility and hemostatic performance of diatom silica, a series of chitosan-coated diatom (CS-diatom) was developed.
27998147	8	66	theme	±	1336:1336	arg1	time					1324:1327	the shortest clotting time	1302:1327	the shortest clotting time (98.34 ± 26.54 s)	1302:1345	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	8	66	theme	±	1336:1336	arg1	s					1344:1344	98.34 ± 26.54 s	1330:1344	98.34 ± 26.54 s	1330:1344	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	8	67	theme	commercial	1467:1476	arg1	QuikClot					1478:1485	commercial QuikClot zeolite	1467:1493	commercial QuikClot zeolite	1467:1493	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	7	68	dep	induce	1087:1092	arg1	activating					1145:1154	activating	1145:1154	activating the intrinsic coagulation pathway	1145:1188	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	7	68	dep	induce	1087:1092	arg1	coagulation					1217:1227	accelerated blood coagulation	1199:1227	accelerated blood coagulation	1199:1227	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	2	69	theme	various	198:204	arg1	limitations					206:216	various limitations	198:216	various limitations	198:216	Current hemostatic agents still have various limitations and side effects.
27998147	8	70	theme	98.34	1330:1334	arg1	time					1324:1327	the shortest clotting time	1302:1327	the shortest clotting time (98.34 ± 26.54 s)	1302:1345	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	8	70	theme	98.34	1330:1334	arg1	s					1344:1344	98.34 ± 26.54 s	1330:1344	98.34 ± 26.54 s	1330:1344	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	6	71	dep	biocompatibility	765:780	arg1	%					802:802	hemolysis ratio < 5%	783:802	hemolysis ratio < 5%	783:802	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	8	72	dep	model	1408:1412	arg1	compare					1414:1420	compare	1414:1420	compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite	1414:1493	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	8	73	theme	blood	1358:1362	arg1	g					1382:1382	0.31 ± 0.11 g	1370:1382	0.31 ± 0.11 g	1370:1382	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	8	73	theme	blood	1358:1362	arg1	loss					1364:1367	lowest blood loss	1351:1367	lowest blood loss (0.31 ± 0.11 g)	1351:1383	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	6	74	theme	<	799:799	arg1	%					802:802	hemolysis ratio < 5%	783:802	hemolysis ratio < 5%	783:802	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	9	75	theme	mesoporous	1628:1637	arg1	materials					1639:1647	nonsynthetic mesoporous materials	1615:1647	nonsynthetic mesoporous materials	1615:1647	The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
27998147	5	76	theme	CS-diatom	525:533	arg1	composition					510:520	The composition	506:520	The composition of CS-diatom prepared	506:542	The composition of CS-diatom prepared was optimized by in vitro hemocompatibility and blood coagulation evaluation for that prepared with 0.5%, 1%, 3%, and 5% chitosan.
27998147	7	77	theme	accelerated	1199:1209	arg1	coagulation					1217:1227	accelerated blood coagulation	1199:1227	accelerated blood coagulation	1199:1227	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	8	78	theme	zeolite	1487:1493	arg1	QuikClot					1478:1485	commercial QuikClot zeolite	1467:1493	commercial QuikClot zeolite	1467:1493	Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite.
27998147	7	79	theme	mechanism	1010:1018	arg1	study					1020:1024	Further blood coagulation mechanism study	984:1024	Further blood coagulation mechanism study	984:1024	Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation.
27998147	6	80	theme	±	862:862	arg1	times					869:873	24.39 ± 1.53 times	856:873	24.39 ± 1.53 times the weight of liquid	856:894	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	9	81	theme	materials	1639:1647	arg1	understanding					1598:1610	a new understanding	1592:1610	a new understanding of nonsynthetic mesoporous materials	1592:1647	The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
27998147	6	82	from	mg/mL	950:954	arg1	s					943:943	351 ± 14.73 s	931:943	351 ± 14.73 s at 5 mg/mL	931:954	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
27998147	6	82	from	mg/mL	950:954	arg1	32.42s					963:968	248 ± 32.42s	957:968	248 ± 32.42s at 10 mg/mL	957:980	The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL).
26569044	7	0	theme	only	1146:1149	arg1	HA					1151:1152	only HA	1146:1152	only HA	1146:1152	These differences were not evident in earlier time points or substrates containing only HA.
26569044	10	1	theme	marker	1688:1693	arg1	expression					1695:1704	functional marker expression	1677:1704	functional marker expression	1677:1704	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	1	2	theme	hepatocytes	262:272	arg1	failure					251:257	the failure	247:257	the failure of hepatocytes to maintain their phenotype in vitro	247:309	Tissue engineering and cell based liver therapies have utilized primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro.
26569044	10	3	theme	dependent	1741:1749	arg1	adhesion					1667:1674	primary human hepatocyte cell adhesion	1637:1674	primary human hepatocyte cell adhesion	1637:1674	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	10	4	theme	morphological	1711:1723	arg1	characteristics					1725:1739	morphological characteristics	1711:1739	morphological characteristics	1711:1739	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	8	5	from	stiffness	1398:1406	arg1	long-term					1380:1388	long-term	1380:1388	long-term	1380:1388	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	7	6	theme	earlier	1101:1107	arg1	points					1114:1119	earlier time points	1101:1119	earlier time points	1101:1119	These differences were not evident in earlier time points or substrates containing only HA.
26569044	1	7	theme	primary	199:205	arg1	hepatocytes					207:217	primary hepatocytes	199:217	primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro	199:309	Tissue engineering and cell based liver therapies have utilized primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro.
26569044	8	8	theme	nuclear	1218:1224	arg1	HNF4α					1242:1246	HNF4α	1242:1246	HNF4α	1242:1246	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	8	8	theme	nuclear	1218:1224	arg1	alpha					1235:1239	hepatocyte nuclear factor 4 alpha	1207:1239	hepatocyte nuclear factor 4 alpha (HNF4α)	1207:1247	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	8	8	theme	nuclear	1218:1224	arg1	markers					1199:1205	the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin	1184:1259	the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin	1184:1259	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	3	9	theme	HA	562:563	arg1	crosslinking					574:585	HA hydrogel crosslinking	562:585	HA hydrogel crosslinking	562:585	The stiffness of the substrate was modulated by adjusting HA hydrogel crosslinking.
26569044	5	10	theme	stiffnesses	866:876	arg1	range					847:851	a narrow physiologically relevant range	813:851	a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa	813:895	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	9	11	dep	Function	1409:1416	arg1	measured					1422:1429	measured	1422:1429	measured by albumin secretion	1422:1450	Function, as measured by albumin secretion, varied with both stiffness and time in culture and peaked at day 7 at the 1200Pa stiffness, slightly below the stiffness of normal liver ECM at 3000Pa.
26569044	10	12	theme	liver	1775:1779	arg1	ECM					1781:1783	liver ECM	1775:1783	liver ECM	1775:1783	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	5	13	theme	liver	933:937	arg1	ECM					939:941	liver ECM	933:941	liver ECM	933:941	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	6	14	theme	Cell	944:947	arg1	attachment					949:958	Cell attachment	944:958	Cell attachment	944:958	Cell attachment, viability, and organization of the actin cytoskeleton improved with increased stiffness up to 4600Pa.
26569044	5	15	theme	ECM	939:941	arg1	absence					922:928	absence	922:928	absence	922:928	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	5	15	theme	ECM	939:941	arg1	presence					909:916	presence	909:916	presence	909:916	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	4	16	theme	normal	702:707	arg1	ECM					737:739	ECM	737:739	ECM	737:739	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
26569044	4	16	theme	normal	702:707	arg1	matrix					729:734	normal liver extracellular matrix	702:734	normal liver extracellular matrix (ECM)	702:740	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
26569044	1	17	theme	cell	158:161	arg1	therapies					175:183	cell based liver therapies	158:183	cell based liver therapies	158:183	Tissue engineering and cell based liver therapies have utilized primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro.
26569044	2	18	theme	cellular	477:484	arg1	microenvironment					486:501	the normal liver cellular microenvironment	460:501	the normal liver cellular microenvironment	460:501	In order to overcome this challenge, hyaluronic acid (HA) cell culture substrates were formulated to closely mimic the composition and stiffness of the normal liver cellular microenvironment.
26569044	4	19	theme	extracellular	715:727	arg1	ECM					737:739	ECM	737:739	ECM	737:739	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
26569044	4	19	theme	extracellular	715:727	arg1	matrix					729:734	normal liver extracellular matrix	702:734	normal liver extracellular matrix (ECM)	702:740	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
26569044	0	20	theme	human	81:85	arg1	function					98:105	human hepatocyte function	81:105	human hepatocyte function	81:105	Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology.
26569044	8	21	from	day	1312:1314	arg1	stiffness					1299:1307	the 4600Pa stiffness	1288:1307	the 4600Pa stiffness at day 7	1288:1316	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	1	22	theme	liver	169:173	arg1	therapies					175:183	cell based liver therapies	158:183	cell based liver therapies	158:183	Tissue engineering and cell based liver therapies have utilized primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro.
26569044	6	23	theme	cytoskeleton	1002:1013	arg1	viability					961:969	viability	961:969	viability	961:969	Cell attachment, viability, and organization of the actin cytoskeleton improved with increased stiffness up to 4600Pa.
26569044	6	23	theme	cytoskeleton	1002:1013	arg1	organization					976:987	organization	976:987	organization	976:987	Cell attachment, viability, and organization of the actin cytoskeleton improved with increased stiffness up to 4600Pa.
26569044	6	23	theme	cytoskeleton	1002:1013	arg1	attachment					949:958	Cell attachment	944:958	Cell attachment	944:958	Cell attachment, viability, and organization of the actin cytoskeleton improved with increased stiffness up to 4600Pa.
26569044	5	24	dep	presence	909:916	arg1	the					905:907	the	905:907	the	905:907	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	5	25	theme	Primary	743:749	arg1	viability					768:776	Primary human hepatocyte viability	743:776	Primary human hepatocyte viability	743:776	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	5	26	from	range	847:851	arg1	600					883:885	600	883:885	600	883:885	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	9	27	from	3000Pa	1597:1602	arg1	stiffness					1564:1572	the stiffness	1560:1572	the stiffness of normal liver ECM at 3000Pa	1560:1602	Function, as measured by albumin secretion, varied with both stiffness and time in culture and peaked at day 7 at the 1200Pa stiffness, slightly below the stiffness of normal liver ECM at 3000Pa.
26569044	5	28	theme	hepatocyte	757:766	arg1	viability					768:776	Primary human hepatocyte viability	743:776	Primary human hepatocyte viability	743:776	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	2	29	theme	cell	370:373	arg1	substrates					383:392	hyaluronic acid (HA) cell culture substrates	349:392	hyaluronic acid (HA) cell culture substrates	349:392	In order to overcome this challenge, hyaluronic acid (HA) cell culture substrates were formulated to closely mimic the composition and stiffness of the normal liver cellular microenvironment.
26569044	5	30	theme	relevant	838:845	arg1	range					847:851	a narrow physiologically relevant range	813:851	a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa	813:895	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	10	31	theme	hepatocyte	1651:1660	arg1	adhesion					1667:1674	primary human hepatocyte cell adhesion	1637:1674	primary human hepatocyte cell adhesion	1637:1674	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	0	32	theme	hyaluronic	13:22	arg1	gels					29:32	hyaluronic acid gels	13:32	hyaluronic acid gels containing liver extracellular matrix	13:70	Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology.
26569044	2	33	theme	normal	464:469	arg1	microenvironment					486:501	the normal liver cellular microenvironment	460:501	the normal liver cellular microenvironment	460:501	In order to overcome this challenge, hyaluronic acid (HA) cell culture substrates were formulated to closely mimic the composition and stiffness of the normal liver cellular microenvironment.
26569044	7	34	from	points	1114:1119	arg1	evident					1090:1096	evident	1090:1096	evident	1090:1096	These differences were not evident in earlier time points or substrates containing only HA.
26569044	10	35	theme	primary	1637:1643	arg1	adhesion					1667:1674	primary human hepatocyte cell adhesion	1637:1674	primary human hepatocyte cell adhesion	1637:1674	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	0	36	theme	gels	29:32	arg1	Stiffness					0:8	Stiffness	0:8	Stiffness of hyaluronic acid gels containing liver extracellular matrix	0:70	Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology.
26569044	7	37	from	substrates	1124:1133	arg1	evident					1090:1096	evident	1090:1096	evident	1090:1096	These differences were not evident in earlier time points or substrates containing only HA.
26569044	10	38	theme	functional	1677:1686	arg1	expression					1695:1704	functional marker expression	1677:1704	functional marker expression	1677:1704	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	5	39	theme	narrow	815:820	arg1	range					847:851	a narrow physiologically relevant range	813:851	a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa	813:895	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	9	40	theme	liver	1584:1588	arg1	ECM					1590:1592	normal liver ECM	1577:1592	normal liver ECM	1577:1592	Function, as measured by albumin secretion, varied with both stiffness and time in culture and peaked at day 7 at the 1200Pa stiffness, slightly below the stiffness of normal liver ECM at 3000Pa.
26569044	8	41	dep	markers	1199:1205	arg1	HNF4α					1242:1246	HNF4α	1242:1246	HNF4α	1242:1246	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	8	41	dep	markers	1199:1205	arg1	albumin					1253:1259	albumin	1253:1259	albumin	1253:1259	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	8	41	dep	markers	1199:1205	arg1	alpha					1235:1239	hepatocyte nuclear factor 4 alpha	1207:1239	hepatocyte nuclear factor 4 alpha (HNF4α)	1207:1247	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	8	41	dep	markers	1199:1205	arg1	markers					1199:1205	the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin	1184:1259	the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin	1184:1259	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	1	42	theme	due	240:242	arg1	success					232:238	limited success	224:238	limited success due to the failure of hepatocytes to maintain their phenotype in vitro	224:309	Tissue engineering and cell based liver therapies have utilized primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro.
26569044	10	43	attach	presence	1763:1770	arg1	culture					1815:1821	culture	1815:1821	culture	1815:1821	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	10	43	attach	presence	1763:1770	arg1	substrates					1792:1801	gel substrates	1788:1801	gel substrates	1788:1801	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	10	43	attach	presence	1763:1770	arg2	ECM					1781:1783	liver ECM	1775:1783	liver ECM	1775:1783	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	4	44	theme	bioactive	620:628	arg1	molecules					630:638	bioactive molecules	620:638	bioactive molecules within the HA substrate	620:662	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
26569044	2	45	theme	acid	360:363	arg1	substrates					383:392	hyaluronic acid (HA) cell culture substrates	349:392	hyaluronic acid (HA) cell culture substrates	349:392	In order to overcome this challenge, hyaluronic acid (HA) cell culture substrates were formulated to closely mimic the composition and stiffness of the normal liver cellular microenvironment.
26569044	2	46	theme	hyaluronic	349:358	arg1	acid					360:363	hyaluronic acid	349:363	hyaluronic acid (HA) cell culture substrates	349:392	In order to overcome this challenge, hyaluronic acid (HA) cell culture substrates were formulated to closely mimic the composition and stiffness of the normal liver cellular microenvironment.
26569044	2	46	theme	hyaluronic	349:358	arg1	HA					366:367	HA	366:367	HA	366:367	In order to overcome this challenge, hyaluronic acid (HA) cell culture substrates were formulated to closely mimic the composition and stiffness of the normal liver cellular microenvironment.
26569044	8	47	theme	gene	1164:1167	arg1	expression					1169:1178	gene expression	1164:1178	gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin	1164:1259	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	7	48	from	evident	1090:1096	arg1	substrates					1124:1133	substrates	1124:1133	substrates containing only HA	1124:1152	These differences were not evident in earlier time points or substrates containing only HA.
26569044	7	48	from	evident	1090:1096	arg1	points					1114:1119	earlier time points	1101:1119	earlier time points	1101:1119	These differences were not evident in earlier time points or substrates containing only HA.
26569044	7	49	theme	time	1109:1112	arg1	points					1114:1119	earlier time points	1101:1119	earlier time points	1101:1119	These differences were not evident in earlier time points or substrates containing only HA.
26569044	0	50	contain	containing	34:43	arg2	matrix					65:70	liver extracellular matrix	45:70	liver extracellular matrix	45:70	Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology.
26569044	0	50	contain	containing	34:43	arg1	gels					29:32	hyaluronic acid gels	13:32	hyaluronic acid gels containing liver extracellular matrix	13:70	Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology.
26569044	3	51	theme	substrate	525:533	arg1	stiffness					508:516	The stiffness	504:516	The stiffness of the substrate	504:533	The stiffness of the substrate was modulated by adjusting HA hydrogel crosslinking.
26569044	9	52	theme	albumin	1434:1440	arg1	secretion					1442:1450	albumin secretion	1434:1450	albumin secretion	1434:1450	Function, as measured by albumin secretion, varied with both stiffness and time in culture and peaked at day 7 at the 1200Pa stiffness, slightly below the stiffness of normal liver ECM at 3000Pa.
26569044	0	53	theme	liver	45:49	arg1	matrix					65:70	liver extracellular matrix	45:70	liver extracellular matrix	45:70	Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology.
26569044	3	54	theme	hydrogel	565:572	arg1	crosslinking					574:585	HA hydrogel crosslinking	562:585	HA hydrogel crosslinking	562:585	The stiffness of the substrate was modulated by adjusting HA hydrogel crosslinking.
26569044	8	55	theme	hepatocyte	1188:1197	arg1	albumin					1253:1259	albumin	1253:1259	albumin	1253:1259	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	8	55	theme	hepatocyte	1188:1197	arg1	alpha					1235:1239	hepatocyte nuclear factor 4 alpha	1207:1239	hepatocyte nuclear factor 4 alpha (HNF4α)	1207:1247	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	8	55	theme	hepatocyte	1188:1197	arg1	markers					1199:1205	the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin	1184:1259	the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin	1184:1259	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	5	56	theme	substrate	856:864	arg1	stiffnesses					866:876	substrate stiffnesses	856:876	substrate stiffnesses from 600 to 4600Pa	856:895	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	8	57	theme	hepatocyte	1207:1216	arg1	HNF4α					1242:1246	HNF4α	1242:1246	HNF4α	1242:1246	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	8	57	theme	hepatocyte	1207:1216	arg1	alpha					1235:1239	hepatocyte nuclear factor 4 alpha	1207:1239	hepatocyte nuclear factor 4 alpha (HNF4α)	1207:1247	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	8	57	theme	hepatocyte	1207:1216	arg1	markers					1199:1205	the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin	1184:1259	the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin	1184:1259	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	10	58	theme	gel	1788:1790	arg1	substrates					1792:1801	gel substrates	1788:1801	gel substrates	1788:1801	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	1	59	theme	limited	224:230	arg1	success					232:238	limited success	224:238	limited success due to the failure of hepatocytes to maintain their phenotype in vitro	224:309	Tissue engineering and cell based liver therapies have utilized primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro.
26569044	8	60	theme	factor	1226:1231	arg1	HNF4α					1242:1246	HNF4α	1242:1246	HNF4α	1242:1246	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	8	60	theme	factor	1226:1231	arg1	alpha					1235:1239	hepatocyte nuclear factor 4 alpha	1207:1239	hepatocyte nuclear factor 4 alpha (HNF4α)	1207:1247	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	8	60	theme	factor	1226:1231	arg1	markers					1199:1205	the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin	1184:1259	the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin	1184:1259	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	4	61	theme	HA	651:652	arg1	substrate					654:662	the HA substrate	647:662	the HA substrate	647:662	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
26569044	10	62	theme	ECM	1781:1783	arg1	time					1807:1810	time	1807:1810	time	1807:1810	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	10	62	theme	ECM	1781:1783	arg1	presence					1763:1770	the presence	1759:1770	the presence of liver ECM in gel substrates	1759:1801	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	5	63	from	600	883:885	arg1	range					847:851	a narrow physiologically relevant range	813:851	a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa	813:895	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	5	63	from	600	883:885	arg1	stiffnesses					866:876	substrate stiffnesses	856:876	substrate stiffnesses from 600 to 4600Pa	856:895	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	8	64	theme	4600Pa	1292:1297	arg1	stiffness					1299:1307	the 4600Pa stiffness	1288:1307	the 4600Pa stiffness at day 7	1288:1316	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	1	65	theme	Tissue	135:140	arg1	engineering					142:152	Tissue engineering	135:152	Tissue engineering	135:152	Tissue engineering and cell based liver therapies have utilized primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro.
26569044	1	66	used	utilized	190:197	arg2	therapies					175:183	cell based liver therapies	158:183	cell based liver therapies	158:183	Tissue engineering and cell based liver therapies have utilized primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro.
26569044	1	66	used	utilized	190:197	arg2	engineering					142:152	Tissue engineering	135:152	Tissue engineering	135:152	Tissue engineering and cell based liver therapies have utilized primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro.
26569044	7	67	contain	containing	1135:1144	arg1	substrates					1124:1133	substrates	1124:1133	substrates containing only HA	1124:1152	These differences were not evident in earlier time points or substrates containing only HA.
26569044	7	67	contain	containing	1135:1144	arg2	HA					1151:1152	only HA	1146:1152	only HA	1146:1152	These differences were not evident in earlier time points or substrates containing only HA.
26569044	6	68	theme	increased	1029:1037	arg1	stiffness					1039:1047	increased stiffness	1029:1047	increased stiffness up to 4600Pa	1029:1060	Cell attachment, viability, and organization of the actin cytoskeleton improved with increased stiffness up to 4600Pa.
26569044	2	69	theme	microenvironment	486:501	arg1	composition					431:441	composition	431:441	composition	431:441	In order to overcome this challenge, hyaluronic acid (HA) cell culture substrates were formulated to closely mimic the composition and stiffness of the normal liver cellular microenvironment.
26569044	2	69	theme	microenvironment	486:501	arg1	stiffness					447:455	stiffness	447:455	stiffness	447:455	In order to overcome this challenge, hyaluronic acid (HA) cell culture substrates were formulated to closely mimic the composition and stiffness of the normal liver cellular microenvironment.
26569044	0	70	theme	hepatocyte	87:96	arg1	function					98:105	human hepatocyte function	81:105	human hepatocyte function	81:105	Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology.
26569044	1	71	theme	based	163:167	arg1	therapies					175:183	cell based liver therapies	158:183	cell based liver therapies	158:183	Tissue engineering and cell based liver therapies have utilized primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro.
26569044	2	72	theme	liver	471:475	arg1	microenvironment					486:501	the normal liver cellular microenvironment	460:501	the normal liver cellular microenvironment	460:501	In order to overcome this challenge, hyaluronic acid (HA) cell culture substrates were formulated to closely mimic the composition and stiffness of the normal liver cellular microenvironment.
26569044	4	73	theme	liver	709:713	arg1	ECM					737:739	ECM	737:739	ECM	737:739	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
26569044	4	73	theme	liver	709:713	arg1	matrix					729:734	normal liver extracellular matrix	702:734	normal liver extracellular matrix (ECM)	702:740	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
26569044	10	74	from	presence	1763:1770	arg1	culture					1815:1821	culture	1815:1821	culture	1815:1821	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	10	74	from	presence	1763:1770	arg1	substrates					1792:1801	gel substrates	1788:1801	gel substrates	1788:1801	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	6	75	theme	actin	996:1000	arg1	cytoskeleton					1002:1013	the actin cytoskeleton	992:1013	the actin cytoskeleton	992:1013	Cell attachment, viability, and organization of the actin cytoskeleton improved with increased stiffness up to 4600Pa.
26569044	0	76	theme	cell	118:121	arg1	morphology					123:132	cell morphology	118:132	cell morphology	118:132	Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology.
26569044	9	77	theme	1200Pa	1527:1532	arg1	stiffness					1534:1542	the 1200Pa stiffness	1523:1542	the 1200Pa stiffness	1523:1542	Function, as measured by albumin secretion, varied with both stiffness and time in culture and peaked at day 7 at the 1200Pa stiffness, slightly below the stiffness of normal liver ECM at 3000Pa.
26569044	2	78	theme	culture	375:381	arg1	substrates					383:392	hyaluronic acid (HA) cell culture substrates	349:392	hyaluronic acid (HA) cell culture substrates	349:392	In order to overcome this challenge, hyaluronic acid (HA) cell culture substrates were formulated to closely mimic the composition and stiffness of the normal liver cellular microenvironment.
26569044	0	79	theme	acid	24:27	arg1	gels					29:32	hyaluronic acid gels	13:32	hyaluronic acid gels containing liver extracellular matrix	13:70	Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology.
26569044	4	80	with	supplementation	681:695	arg1	ECM					737:739	ECM	737:739	ECM	737:739	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
26569044	4	80	with	supplementation	681:695	arg1	matrix					729:734	normal liver extracellular matrix	702:734	normal liver extracellular matrix (ECM)	702:740	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
26569044	8	81	theme	long-term	1380:1388	arg1	phenotype					1370:1378	their phenotype	1364:1378	their phenotype long-term at this stiffness	1364:1406	However, gene expression for the hepatocyte markers hepatocyte nuclear factor 4 alpha (HNF4α) and albumin significantly decreased on the 4600Pa stiffness at day 7 indicating that cells may not have maintained their phenotype long-term at this stiffness.
26569044	10	82	theme	human	1645:1649	arg1	adhesion					1667:1674	primary human hepatocyte cell adhesion	1637:1674	primary human hepatocyte cell adhesion	1637:1674	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	1	83	with	hepatocytes	207:217	arg1	success					232:238	limited success	224:238	limited success due to the failure of hepatocytes to maintain their phenotype in vitro	224:309	Tissue engineering and cell based liver therapies have utilized primary hepatocytes with limited success due to the failure of hepatocytes to maintain their phenotype in vitro.
26569044	10	84	from	time	1807:1810	arg1	culture					1815:1821	culture	1815:1821	culture	1815:1821	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	10	84	from	time	1807:1810	arg1	substrates					1792:1801	gel substrates	1788:1801	gel substrates	1788:1801	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	0	85	theme	extracellular	51:63	arg1	matrix					65:70	liver extracellular matrix	45:70	liver extracellular matrix	45:70	Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology.
26569044	9	86	theme	normal	1577:1582	arg1	ECM					1590:1592	normal liver ECM	1577:1592	normal liver ECM	1577:1592	Function, as measured by albumin secretion, varied with both stiffness and time in culture and peaked at day 7 at the 1200Pa stiffness, slightly below the stiffness of normal liver ECM at 3000Pa.
26569044	9	87	theme	ECM	1590:1592	arg1	stiffness					1564:1572	the stiffness	1560:1572	the stiffness of normal liver ECM at 3000Pa	1560:1602	Function, as measured by albumin secretion, varied with both stiffness and time in culture and peaked at day 7 at the 1200Pa stiffness, slightly below the stiffness of normal liver ECM at 3000Pa.
26569044	5	88	theme	human	751:755	arg1	viability					768:776	Primary human hepatocyte viability	743:776	Primary human hepatocyte viability	743:776	Primary human hepatocyte viability and phenotype were determined over a narrow physiologically relevant range of substrate stiffnesses from 600 to 4600Pa in both the presence and absence of liver ECM.
26569044	10	89	theme	cell	1662:1665	arg1	adhesion					1667:1674	primary human hepatocyte cell adhesion	1637:1674	primary human hepatocyte cell adhesion	1637:1674	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	10	90	theme	gel	1614:1616	arg1	stiffness					1618:1626	gel stiffness	1614:1626	gel stiffness	1614:1626	Overall, gel stiffness affected primary human hepatocyte cell adhesion, functional marker expression, and morphological characteristics dependent on both the presence of liver ECM in gel substrates and time in culture.
26569044	4	91	theme	molecules	630:638	arg1	repertoire					606:615	the repertoire	602:615	the repertoire of bioactive molecules within the HA substrate	602:662	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
26569044	4	91	theme	molecules	630:638	arg1	molecules					630:638	bioactive molecules	620:638	bioactive molecules within the HA substrate	620:662	Additionally, the repertoire of bioactive molecules within the HA substrate was bolstered by supplementation with normal liver extracellular matrix (ECM).
25782578	7	0	theme	P	1427:1427	arg1	fish					1421:1424	fish	1421:1424	fish (P < 0.05)	1421:1435	Furthermore, intestinal microflora was analyzed by PCR-DGGE, and the results showed that OKGM diets also significantly modulated the intestinal microflora of fish (P < 0.05).
25782578	7	0	theme	P	1427:1427	arg1	<					1429:1429	P < 0.05	1427:1434	P < 0.05	1427:1434	Furthermore, intestinal microflora was analyzed by PCR-DGGE, and the results showed that OKGM diets also significantly modulated the intestinal microflora of fish (P < 0.05).
25782578	5	1	dep	better	931:936	arg1	<					941:941	P < 0.05	939:946	P < 0.05	939:946	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	5	2	theme	muscularis	1095:1104	arg1	thickness					1106:1114	circular muscularis thickness	1086:1114	circular muscularis thickness	1086:1114	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	4	3	theme	lipid	758:762	arg1	content					733:739	The content	729:739	The content of body protein, lipid and moisture	729:775	The content of body protein, lipid and moisture was affected by the OKGM diets.
25782578	8	4	theme	intestinal	1525:1534	arg1	morphology					1536:1545	intestinal morphology	1525:1545	intestinal morphology	1525:1545	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	5	5	theme	longitudinal	1048:1059	arg1	thickness					1072:1080	longitudinal muscularis thickness	1048:1080	longitudinal muscularis thickness	1048:1080	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	5	6	theme	intestinal	861:870	arg1	morphology					872:881	intestinal morphology	861:881	intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet	861:925	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	5	7	theme	P	939:939	arg1	<					941:941	P < 0.05	939:946	P < 0.05	939:946	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	8	8	theme	g	1659:1659	arg1	-1					1664:1665	-1	1664:1665	-1	1664:1665	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	8	8	theme	g	1659:1659	arg1	kg					1661:1662	16.0 g kg	1654:1662	16.0 g kg(-1)	1654:1666	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	8	8	theme	g	1659:1659	arg1	levels					1627:1632	the optimal dietary OKGM levels	1602:1632	the optimal dietary OKGM levels	1602:1632	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	1	9	theme	intestinal	340:349	arg1	microflora					351:360	intestinal microflora	340:360	intestinal microflora	340:360	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	6	10	theme	OKGM	1173:1176	arg1	diet					1178:1181	32.0 g kg(-1) OKGM diet	1159:1181	32.0 g kg(-1) OKGM diet	1159:1181	Compared with the control group, fish fed 32.0 g kg(-1) OKGM diet showed significantly lower goblet cell number in anterior intestine (P < 0.05).
25782578	5	11	theme	mucosa	983:988	arg1	height					995:1000	mucosa fold height	983:1000	mucosa fold height	983:1000	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	0	12	theme	body	91:94	arg1	composition					96:106	body composition	91:106	body composition	91:106	Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	6	13	theme	anterior	1232:1239	arg1	intestine					1241:1249	anterior intestine	1232:1249	anterior intestine (P < 0.05)	1232:1260	Compared with the control group, fish fed 32.0 g kg(-1) OKGM diet showed significantly lower goblet cell number in anterior intestine (P < 0.05).
25782578	6	13	theme	anterior	1232:1239	arg1	<					1254:1254	P < 0.05	1252:1259	P < 0.05	1252:1259	Compared with the control group, fish fed 32.0 g kg(-1) OKGM diet showed significantly lower goblet cell number in anterior intestine (P < 0.05).
25782578	4	14	theme	moisture	768:775	arg1	content					733:739	The content	729:739	The content of body protein, lipid and moisture	729:775	The content of body protein, lipid and moisture was affected by the OKGM diets.
25782578	5	15	theme	muscularis	1061:1070	arg1	thickness					1072:1080	longitudinal muscularis thickness	1048:1080	longitudinal muscularis thickness	1048:1080	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	0	16	theme	intestinal	109:118	arg1	morphology					120:129	intestinal morphology	109:129	intestinal morphology	109:129	Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	3	17	theme	P	718:718	arg1	diet					712:715	16.0 g kg(-1) OKGM diet	693:715	16.0 g kg(-1) OKGM diet (P < 0.05)	693:726	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	3	17	theme	P	718:718	arg1	<					720:720	P < 0.05	718:725	P < 0.05	718:725	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	5	18	theme	fold	990:993	arg1	height					995:1000	mucosa fold height	983:1000	mucosa fold height	983:1000	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	5	19	theme	fish	886:889	arg1	morphology					872:881	intestinal morphology	861:881	intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet	861:925	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	8	20	theme	intestinal	1560:1569	arg1	microflora					1571:1580	intestinal microflora	1560:1580	intestinal microflora of S. prenanti	1560:1595	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	7	21	theme	intestinal	1396:1405	arg1	microflora					1407:1416	the intestinal microflora	1392:1416	the intestinal microflora of fish (P < 0.05)	1392:1435	Furthermore, intestinal microflora was analyzed by PCR-DGGE, and the results showed that OKGM diets also significantly modulated the intestinal microflora of fish (P < 0.05).
25782578	7	22	theme	intestinal	1276:1285	arg1	microflora					1287:1296	intestinal microflora	1276:1296	intestinal microflora	1276:1296	Furthermore, intestinal microflora was analyzed by PCR-DGGE, and the results showed that OKGM diets also significantly modulated the intestinal microflora of fish (P < 0.05).
25782578	3	23	theme	OKGM	707:710	arg1	diet					712:715	16.0 g kg(-1) OKGM diet	693:715	16.0 g kg(-1) OKGM diet (P < 0.05)	693:726	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	3	23	theme	OKGM	707:710	arg1	<					720:720	P < 0.05	718:725	P < 0.05	718:725	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	1	24	theme	present	173:179	arg1	study					181:185	the present study	169:185	the present study	169:185	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	4	25	theme	OKGM	797:800	arg1	diets					802:806	the OKGM diets	793:806	the OKGM diets	793:806	The content of body protein, lipid and moisture was affected by the OKGM diets.
25782578	3	26	theme	basal	604:608	arg1	diet					610:613	basal diet	604:613	basal diet	604:613	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	5	27	theme	mucosal	1003:1009	arg1	height					1022:1027	mucosal epithelial height	1003:1027	mucosal epithelial height	1003:1027	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	5	28	theme	OKGM	917:920	arg1	diet					922:925	8.0 and 16.0 g kg(-1) OKGM diet	895:925	8.0 and 16.0 g kg(-1) OKGM diet	895:925	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	1	29	theme	study	181:185	arg1	aim					162:164	The aim	158:164	The aim of the present study	158:185	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	1	30	theme	Schizothorax	254:265	arg1	performance					283:293	Schizothorax prenanti growth performance	254:293	Schizothorax prenanti growth performance	254:293	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	5	31	theme	g	908:908	arg1	kg					910:911	8.0 and 16.0 g kg(-1)	895:915	8.0 and 16.0 g kg(-1) OKGM diet	895:925	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	5	32	theme	epithelial	1011:1020	arg1	height					1022:1027	mucosal epithelial height	1003:1027	mucosal epithelial height	1003:1027	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	0	33	theme	oxidized	18:25	arg1	konjac					27:32	dietary oxidized konjac	10:32	dietary oxidized konjac	10:32	Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	1	34	theme	prenanti	267:274	arg1	performance					283:293	Schizothorax prenanti growth performance	254:293	Schizothorax prenanti growth performance	254:293	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	6	35	theme	cell	1217:1220	arg1	number					1222:1227	significantly lower goblet cell number	1190:1227	significantly lower goblet cell number	1190:1227	Compared with the control group, fish fed 32.0 g kg(-1) OKGM diet showed significantly lower goblet cell number in anterior intestine (P < 0.05).
25782578	0	36	theme	dietary	10:16	arg1	konjac					27:32	dietary oxidized konjac	10:32	dietary oxidized konjac	10:32	Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	1	37	theme	growth	276:281	arg1	performance					283:293	Schizothorax prenanti growth performance	254:293	Schizothorax prenanti growth performance	254:293	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	8	38	theme	prenanti	1588:1595	arg1	microflora					1571:1580	intestinal microflora	1560:1580	intestinal microflora of S. prenanti	1560:1595	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	3	39	theme	kg	552:553	arg1	<					573:573	P < 0.05	571:578	P < 0.05	571:578	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	3	39	theme	kg	552:553	arg1	diets					564:568	8.0 and 16.0 g kg(-1) OKGM diets	537:568	8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05)	537:579	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	6	40	theme	lower	1204:1208	arg1	number					1222:1227	significantly lower goblet cell number	1190:1227	significantly lower goblet cell number	1190:1227	Compared with the control group, fish fed 32.0 g kg(-1) OKGM diet showed significantly lower goblet cell number in anterior intestine (P < 0.05).
25782578	6	41	theme	goblet	1210:1215	arg1	number					1222:1227	significantly lower goblet cell number	1190:1227	significantly lower goblet cell number	1190:1227	Compared with the control group, fish fed 32.0 g kg(-1) OKGM diet showed significantly lower goblet cell number in anterior intestine (P < 0.05).
25782578	2	42	theme	g	433:433	arg1	-1					438:439	-1	438:439	-1	438:439	Fish were fed a basal diet or basal diet plus 4.0, 8.0, 16.0 and 32.0 g kg(-1) OKGM for 60 days.
25782578	2	42	theme	g	433:433	arg1	kg					435:436	4.0, 8.0, 16.0 and 32.0 g kg	409:436	4.0, 8.0, 16.0 and 32.0 g kg(-1)	409:440	Fish were fed a basal diet or basal diet plus 4.0, 8.0, 16.0 and 32.0 g kg(-1) OKGM for 60 days.
25782578	8	43	theme	growth	1497:1502	arg1	performance					1504:1514	the growth performance	1493:1514	the growth performance	1493:1514	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	5	44	theme	submucosa	1030:1038	arg1	height					1040:1045	submucosa height	1030:1045	submucosa height	1030:1045	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	2	45	theme	basal	393:397	arg1	diet					399:402	basal diet	393:402	basal diet	393:402	Fish were fed a basal diet or basal diet plus 4.0, 8.0, 16.0 and 32.0 g kg(-1) OKGM for 60 days.
25782578	0	46	theme	konjac	27:32	arg1	Effect					0:5	Effect	0:5	Effect of dietary oxidized konjac	0:32	Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	5	47	theme	kg	910:911	arg1	diet					922:925	8.0 and 16.0 g kg(-1) OKGM diet	895:925	8.0 and 16.0 g kg(-1) OKGM diet	895:925	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	0	48	theme	intestinal	135:144	arg1	microflora					146:155	intestinal microflora	135:155	intestinal microflora	135:155	Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	0	49	theme	Schizothorax	49:60	arg1	performance					78:88	Schizothorax prenanti growth performance	49:88	Schizothorax prenanti growth performance	49:88	Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	1	50	theme	body	296:299	arg1	composition					301:311	body composition	296:311	body composition	296:311	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	8	51	theme	dietary	1614:1620	arg1	kg					1661:1662	16.0 g kg	1654:1662	16.0 g kg(-1)	1654:1666	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	8	51	theme	dietary	1614:1620	arg1	levels					1627:1632	the optimal dietary OKGM levels	1602:1632	the optimal dietary OKGM levels	1602:1632	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	3	52	theme	g	550:550	arg1	kg					552:553	8.0 and 16.0 g kg	537:553	8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05)	537:579	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	3	52	theme	g	550:550	arg1	-1					555:556	-1	555:556	-1	555:556	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	1	53	from	effect	206:211	arg1	performance					283:293	Schizothorax prenanti growth performance	254:293	Schizothorax prenanti growth performance	254:293	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	1	53	from	effect	206:211	arg1	composition					301:311	body composition	296:311	body composition	296:311	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	1	53	from	effect	206:211	arg1	morphology					325:334	intestinal morphology	314:334	intestinal morphology	314:334	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	1	53	from	effect	206:211	arg1	microflora					351:360	intestinal microflora	340:360	intestinal microflora	340:360	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	3	54	theme	P	571:571	arg1	<					573:573	P < 0.05	571:578	P < 0.05	571:578	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	3	54	theme	P	571:571	arg1	diets					564:568	8.0 and 16.0 g kg(-1) OKGM diets	537:568	8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05)	537:579	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	6	55	theme	control	1135:1141	arg1	group					1143:1147	the control group	1131:1147	the control group	1131:1147	Compared with the control group, fish fed 32.0 g kg(-1) OKGM diet showed significantly lower goblet cell number in anterior intestine (P < 0.05).
25782578	8	56	theme	optimal	1606:1612	arg1	kg					1661:1662	16.0 g kg	1654:1662	16.0 g kg(-1)	1654:1666	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	8	56	theme	optimal	1606:1612	arg1	levels					1627:1632	the optimal dietary OKGM levels	1602:1632	the optimal dietary OKGM levels	1602:1632	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	3	57	theme	OKGM	559:562	arg1	<					573:573	P < 0.05	571:578	P < 0.05	571:578	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	3	57	theme	OKGM	559:562	arg1	diets					564:568	8.0 and 16.0 g kg(-1) OKGM diets	537:568	8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05)	537:579	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	4	58	theme	protein	749:755	arg1	content					733:739	The content	729:739	The content of body protein, lipid and moisture	729:775	The content of body protein, lipid and moisture was affected by the OKGM diets.
25782578	4	59	theme	body	744:747	arg1	protein					749:755	body protein	744:755	body protein	744:755	The content of body protein, lipid and moisture was affected by the OKGM diets.
25782578	5	60	theme	light	813:817	arg1	microscopy					832:841	The light and electron microscopy	809:841	microscopy	832:841	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	3	61	theme	g	698:698	arg1	diet					712:715	16.0 g kg(-1) OKGM diet	693:715	16.0 g kg(-1) OKGM diet (P < 0.05)	693:726	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	3	61	theme	g	698:698	arg1	<					720:720	P < 0.05	718:725	P < 0.05	718:725	The results indicated that WGR and SGR were significantly higher in fish fed 8.0 and 16.0 g kg(-1) OKGM diets (P < 0.05) than those in fish fed basal diet, and PER was significantly higher and FCR was significantly lower in fish fed 16.0 g kg(-1) OKGM diet (P < 0.05).
25782578	8	62	theme	OKGM	1622:1625	arg1	kg					1661:1662	16.0 g kg	1654:1662	16.0 g kg(-1)	1654:1666	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	8	62	theme	OKGM	1622:1625	arg1	levels					1627:1632	the optimal dietary OKGM levels	1602:1632	the optimal dietary OKGM levels	1602:1632	The study clearly demonstrates that OKGM could enhance the growth performance, improve intestinal morphology and modulate intestinal microflora of S. prenanti, and the optimal dietary OKGM levels was suggested to be 16.0 g kg(-1).
25782578	6	63	theme	P	1252:1252	arg1	intestine					1241:1249	anterior intestine	1232:1249	anterior intestine (P < 0.05)	1232:1260	Compared with the control group, fish fed 32.0 g kg(-1) OKGM diet showed significantly lower goblet cell number in anterior intestine (P < 0.05).
25782578	6	63	theme	P	1252:1252	arg1	<					1254:1254	P < 0.05	1252:1259	P < 0.05	1252:1259	Compared with the control group, fish fed 32.0 g kg(-1) OKGM diet showed significantly lower goblet cell number in anterior intestine (P < 0.05).
25782578	7	64	theme	OKGM	1352:1355	arg1	diets					1357:1361	OKGM diets	1352:1361	OKGM diets	1352:1361	Furthermore, intestinal microflora was analyzed by PCR-DGGE, and the results showed that OKGM diets also significantly modulated the intestinal microflora of fish (P < 0.05).
25782578	7	65	theme	fish	1421:1424	arg1	microflora					1407:1416	the intestinal microflora	1392:1416	the intestinal microflora of fish (P < 0.05)	1392:1435	Furthermore, intestinal microflora was analyzed by PCR-DGGE, and the results showed that OKGM diets also significantly modulated the intestinal microflora of fish (P < 0.05).
25782578	1	66	theme	oxidized	216:223	arg1	OKGM					245:248	OKGM	245:248	OKGM	245:248	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	1	66	theme	oxidized	216:223	arg1	glucomannan					232:242	oxidized konjac glucomannan	216:242	oxidized konjac glucomannan (OKGM)	216:249	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	0	67	theme	growth	71:76	arg1	performance					78:88	Schizothorax prenanti growth performance	49:88	Schizothorax prenanti growth performance	49:88	Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	5	68	theme	electron	823:830	arg1	microscopy					832:841	The light and electron microscopy	809:841	microscopy	832:841	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	2	69	dep	diet	385:388	arg1	OKGM					442:445	OKGM	442:445	OKGM	442:445	Fish were fed a basal diet or basal diet plus 4.0, 8.0, 16.0 and 32.0 g kg(-1) OKGM for 60 days.
25782578	2	70	theme	basal	379:383	arg1	diet					385:388	a basal diet	377:388	a basal diet	377:388	Fish were fed a basal diet or basal diet plus 4.0, 8.0, 16.0 and 32.0 g kg(-1) OKGM for 60 days.
25782578	1	71	theme	konjac	225:230	arg1	OKGM					245:248	OKGM	245:248	OKGM	245:248	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	1	71	theme	konjac	225:230	arg1	glucomannan					232:242	oxidized konjac glucomannan	216:242	oxidized konjac glucomannan (OKGM)	216:249	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	0	72	theme	prenanti	62:69	arg1	performance					78:88	Schizothorax prenanti growth performance	49:88	Schizothorax prenanti growth performance	49:88	Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	1	73	theme	intestinal	314:323	arg1	morphology					325:334	intestinal morphology	314:334	intestinal morphology	314:334	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
25782578	5	74	theme	control	958:964	arg1	group					966:970	the control group	954:970	the control group	954:970	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	5	75	theme	circular	1086:1093	arg1	thickness					1106:1114	circular muscularis thickness	1086:1114	circular muscularis thickness	1086:1114	The light and electron microscopy demonstrated that intestinal morphology of fish fed 8.0 and 16.0 g kg(-1) OKGM diet was better (P < 0.05) than the control group, including mucosa fold height, mucosal epithelial height, submucosa height, longitudinal muscularis thickness and circular muscularis thickness.
25782578	6	76	theme	g	1164:1164	arg1	diet					1178:1181	32.0 g kg(-1) OKGM diet	1159:1181	32.0 g kg(-1) OKGM diet	1159:1181	Compared with the control group, fish fed 32.0 g kg(-1) OKGM diet showed significantly lower goblet cell number in anterior intestine (P < 0.05).
25782578	1	77	theme	glucomannan	232:242	arg1	effect					206:211	the effect	202:211	the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora	202:360	The aim of the present study was to examine the effect of oxidized konjac glucomannan (OKGM) on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora.
26197284	0	0	theme	Bod	80:82	arg1	Cheese					90:95	Bod Ljong Cheese	80:95	Bod Ljong Cheese	80:95	Evaluation of Chitosan-Starch-Based Edible Coating To Improve the Shelf Life of Bod Ljong Cheese.
26197284	1	1	theme	cheese	228:233	arg1	quality					207:213	the quality	203:213	the quality of Bod ljong cheese	203:233	The objective of this work was to evaluate the effectiveness of antimicrobial edible coatings to improve the quality of Bod ljong cheese throughout 25 days of storage.
26197284	0	2	theme	Cheese	90:95	arg1	Life					72:75	the Shelf Life	62:75	the Shelf Life of Bod Ljong Cheese	62:95	Evaluation of Chitosan-Starch-Based Edible Coating To Improve the Shelf Life of Bod Ljong Cheese.
26197284	4	3	theme	microorganisms	744:757	arg1	growth					734:739	growth	734:739	growth of microorganisms	734:757	Moreover, the edible coatings with COFE or PNEO had increased antimicrobial activity and did not permit growth of microorganisms.
26197284	5	4	theme	cheese	868:873	arg1	they					820:823	they	820:823	they	820:823	COFE and PNEO are manufactured from food-grade materials so they can be consumed as an integral part of the cheese, which represents a competitive advantage over nonedible coatings.
26197284	5	4	theme	cheese	868:873	arg1	part					856:859	an integral part	844:859	an integral part of the cheese, which represents a competitive advantage over nonedible coatings	844:939	COFE and PNEO are manufactured from food-grade materials so they can be consumed as an integral part of the cheese, which represents a competitive advantage over nonedible coatings.
26197284	0	5	theme	Ljong	84:88	arg1	Cheese					90:95	Bod Ljong Cheese	80:95	Bod Ljong Cheese	80:95	Evaluation of Chitosan-Starch-Based Edible Coating To Improve the Shelf Life of Bod Ljong Cheese.
26197284	2	6	theme	needle	469:474	arg1	PNEO					491:494	PNEO	491:494	PNEO	491:494	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	6	theme	needle	469:474	arg1	oil					486:488	pine needle essential oil	464:488	pine needle essential oil (PNEO)	464:495	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	4	7	theme	antimicrobial	692:704	arg1	activity					706:713	increased antimicrobial activity	682:713	increased antimicrobial activity	682:713	Moreover, the edible coatings with COFE or PNEO had increased antimicrobial activity and did not permit growth of microorganisms.
26197284	2	8	theme	base	346:349	arg1	matrix					351:356	a base matrix	344:356	a base matrix	344:356	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	8	theme	base	346:349	arg1	starch					320:325	water chestnut starch	305:325	water chestnut starch	305:325	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	8	theme	base	346:349	arg1	glycerol					332:339	glycerol	332:339	glycerol	332:339	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	8	theme	base	346:349	arg1	chitosan					295:302	chitosan	295:302	chitosan	295:302	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	9	theme	pine	464:467	arg1	needle					469:474	pine needle	464:474	pine needle essential oil (PNEO)	464:495	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	4	10	theme	increased	682:690	arg1	activity					706:713	increased antimicrobial activity	682:713	increased antimicrobial activity	682:713	Moreover, the edible coatings with COFE or PNEO had increased antimicrobial activity and did not permit growth of microorganisms.
26197284	3	11	from	loss	558:561	arg1	composition					606:616	headspace gas composition	592:616	headspace gas composition	592:616	Application of coating on cheese decreased water loss, lipid oxidation, changes in headspace gas composition, and color.
26197284	5	12	theme	integral	847:854	arg1	they					820:823	they	820:823	they	820:823	COFE and PNEO are manufactured from food-grade materials so they can be consumed as an integral part of the cheese, which represents a competitive advantage over nonedible coatings.
26197284	5	12	theme	integral	847:854	arg1	part					856:859	an integral part	844:859	an integral part of the cheese, which represents a competitive advantage over nonedible coatings	844:939	COFE and PNEO are manufactured from food-grade materials so they can be consumed as an integral part of the cheese, which represents a competitive advantage over nonedible coatings.
26197284	1	13	theme	antimicrobial	162:174	arg1	coatings					183:190	antimicrobial edible coatings	162:190	antimicrobial edible coatings	162:190	The objective of this work was to evaluate the effectiveness of antimicrobial edible coatings to improve the quality of Bod ljong cheese throughout 25 days of storage.
26197284	2	14	theme	substances	411:420	arg1	combinations					381:392	several combinations	373:392	several combinations of antimicrobial substances	373:420	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	1	15	theme	edible	176:181	arg1	coatings					183:190	antimicrobial edible coatings	162:190	antimicrobial edible coatings	162:190	The objective of this work was to evaluate the effectiveness of antimicrobial edible coatings to improve the quality of Bod ljong cheese throughout 25 days of storage.
26197284	0	16	theme	Edible	36:41	arg1	Coating					43:49	Chitosan-Starch-Based Edible Coating	14:49	Chitosan-Starch-Based Edible Coating	14:49	Evaluation of Chitosan-Starch-Based Edible Coating To Improve the Shelf Life of Bod Ljong Cheese.
26197284	1	17	theme	storage	257:263	arg1	days					249:252	25 days	246:252	25 days of storage	246:263	The objective of this work was to evaluate the effectiveness of antimicrobial edible coatings to improve the quality of Bod ljong cheese throughout 25 days of storage.
26197284	1	18	theme	coatings	183:190	arg1	effectiveness					145:157	the effectiveness	141:157	the effectiveness of antimicrobial edible coatings to improve the quality of Bod ljong cheese throughout 25 days of storage	141:263	The objective of this work was to evaluate the effectiveness of antimicrobial edible coatings to improve the quality of Bod ljong cheese throughout 25 days of storage.
26197284	0	19	theme	Chitosan-Starch-Based	14:34	arg1	Coating					43:49	Chitosan-Starch-Based Edible Coating	14:49	Chitosan-Starch-Based Edible Coating	14:49	Evaluation of Chitosan-Starch-Based Edible Coating To Improve the Shelf Life of Bod Ljong Cheese.
26197284	2	20	theme	several	373:379	arg1	combinations					381:392	several combinations	373:392	several combinations of antimicrobial substances	373:420	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	5	21	theme	nonedible	922:930	arg1	coatings					932:939	nonedible coatings	922:939	nonedible coatings	922:939	COFE and PNEO are manufactured from food-grade materials so they can be consumed as an integral part of the cheese, which represents a competitive advantage over nonedible coatings.
26197284	5	22	theme	food-grade	796:805	arg1	materials					807:815	food-grade materials	796:815	food-grade materials	796:815	COFE and PNEO are manufactured from food-grade materials so they can be consumed as an integral part of the cheese, which represents a competitive advantage over nonedible coatings.
26197284	2	23	theme	fruit	442:446	arg1	COFE					457:460	COFE	457:460	COFE	457:460	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	23	theme	fruit	442:446	arg1	extract					448:454	Cornus officinalis fruit extract	423:454	Cornus officinalis fruit extract (COFE)	423:461	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	0	24	theme	Coating	43:49	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Chitosan-Starch-Based Edible Coating	0:49	Evaluation of Chitosan-Starch-Based Edible Coating To Improve the Shelf Life of Bod Ljong Cheese.
26197284	3	25	theme	lipid	564:568	arg1	oxidation					570:578	lipid oxidation	564:578	lipid oxidation	564:578	Application of coating on cheese decreased water loss, lipid oxidation, changes in headspace gas composition, and color.
26197284	2	26	theme	antimicrobial	397:409	arg1	substances					411:420	antimicrobial substances	397:420	antimicrobial substances	397:420	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	3	27	from	changes	581:587	arg1	composition					606:616	headspace gas composition	592:616	headspace gas composition	592:616	Application of coating on cheese decreased water loss, lipid oxidation, changes in headspace gas composition, and color.
26197284	2	28	theme	officinalis	430:440	arg1	COFE					457:460	COFE	457:460	COFE	457:460	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	28	theme	officinalis	430:440	arg1	extract					448:454	Cornus officinalis fruit extract	423:454	Cornus officinalis fruit extract (COFE)	423:461	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	29	theme	essential	476:484	arg1	PNEO					491:494	PNEO	491:494	PNEO	491:494	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	29	theme	essential	476:484	arg1	oil					486:488	pine needle essential oil	464:488	pine needle essential oil (PNEO)	464:495	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	3	30	from	color	623:627	arg1	composition					606:616	headspace gas composition	592:616	headspace gas composition	592:616	Application of coating on cheese decreased water loss, lipid oxidation, changes in headspace gas composition, and color.
26197284	2	31	dep	prepared	280:287	arg1	COFE					457:460	COFE	457:460	COFE	457:460	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	31	dep	prepared	280:287	arg1	nisin					502:506	nisin	502:506	nisin	502:506	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	31	dep	prepared	280:287	arg1	extract					448:454	Cornus officinalis fruit extract	423:454	Cornus officinalis fruit extract (COFE)	423:461	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	31	dep	prepared	280:287	arg1	PNEO					491:494	PNEO	491:494	PNEO	491:494	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	31	dep	prepared	280:287	arg1	oil					486:488	pine needle essential oil	464:488	pine needle essential oil (PNEO)	464:495	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	32	theme	Cornus	423:428	arg1	COFE					457:460	COFE	457:460	COFE	457:460	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	32	theme	Cornus	423:428	arg1	extract					448:454	Cornus officinalis fruit extract	423:454	Cornus officinalis fruit extract (COFE)	423:461	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	3	33	theme	gas	602:604	arg1	composition					606:616	headspace gas composition	592:616	headspace gas composition	592:616	Application of coating on cheese decreased water loss, lipid oxidation, changes in headspace gas composition, and color.
26197284	3	34	theme	coating	524:530	arg1	Application					509:519	Application	509:519	Application of coating on cheese	509:540	Application of coating on cheese decreased water loss, lipid oxidation, changes in headspace gas composition, and color.
26197284	2	35	theme	chestnut	311:318	arg1	matrix					351:356	a base matrix	344:356	a base matrix	344:356	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	35	theme	chestnut	311:318	arg1	starch					320:325	water chestnut starch	305:325	water chestnut starch	305:325	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	35	theme	chestnut	311:318	arg1	glycerol					332:339	glycerol	332:339	glycerol	332:339	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	35	theme	chestnut	311:318	arg1	chitosan					295:302	chitosan	295:302	chitosan	295:302	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	4	36	contain	had	678:680	arg2	activity					706:713	increased antimicrobial activity	682:713	increased antimicrobial activity	682:713	Moreover, the edible coatings with COFE or PNEO had increased antimicrobial activity and did not permit growth of microorganisms.
26197284	4	36	contain	had	678:680	arg1	coatings					651:658	the edible coatings	640:658	the edible coatings with COFE or PNEO	640:676	Moreover, the edible coatings with COFE or PNEO had increased antimicrobial activity and did not permit growth of microorganisms.
26197284	2	37	theme	water	305:309	arg1	matrix					351:356	a base matrix	344:356	a base matrix	344:356	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	37	theme	water	305:309	arg1	starch					320:325	water chestnut starch	305:325	water chestnut starch	305:325	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	37	theme	water	305:309	arg1	glycerol					332:339	glycerol	332:339	glycerol	332:339	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	2	37	theme	water	305:309	arg1	chitosan					295:302	chitosan	295:302	chitosan	295:302	Coatings were prepared using chitosan, water chestnut starch, and glycerol as a base matrix, together with several combinations of antimicrobial substances: Cornus officinalis fruit extract (COFE), pine needle essential oil (PNEO), and nisin.
26197284	5	38	theme	competitive	895:905	arg1	advantage					907:915	a competitive advantage	893:915	a competitive advantage over nonedible coatings	893:939	COFE and PNEO are manufactured from food-grade materials so they can be consumed as an integral part of the cheese, which represents a competitive advantage over nonedible coatings.
26197284	1	39	theme	work	120:123	arg1	objective					102:110	The objective	98:110	The objective of this work	98:123	The objective of this work was to evaluate the effectiveness of antimicrobial edible coatings to improve the quality of Bod ljong cheese throughout 25 days of storage.
26197284	4	40	theme	edible	644:649	arg1	coatings					651:658	the edible coatings	640:658	the edible coatings with COFE or PNEO	640:676	Moreover, the edible coatings with COFE or PNEO had increased antimicrobial activity and did not permit growth of microorganisms.
26197284	3	41	theme	headspace	592:600	arg1	composition					606:616	headspace gas composition	592:616	headspace gas composition	592:616	Application of coating on cheese decreased water loss, lipid oxidation, changes in headspace gas composition, and color.
26197284	4	42	with	coatings	651:658	arg1	COFE					665:668	COFE	665:668	COFE	665:668	Moreover, the edible coatings with COFE or PNEO had increased antimicrobial activity and did not permit growth of microorganisms.
26197284	4	42	with	coatings	651:658	arg1	PNEO					673:676	PNEO	673:676	PNEO	673:676	Moreover, the edible coatings with COFE or PNEO had increased antimicrobial activity and did not permit growth of microorganisms.
26197284	3	43	from	Application	509:519	arg1	cheese					535:540	cheese	535:540	cheese	535:540	Application of coating on cheese decreased water loss, lipid oxidation, changes in headspace gas composition, and color.
26197284	1	44	theme	Bod	218:220	arg1	cheese					228:233	Bod ljong cheese	218:233	Bod ljong cheese	218:233	The objective of this work was to evaluate the effectiveness of antimicrobial edible coatings to improve the quality of Bod ljong cheese throughout 25 days of storage.
26197284	0	45	theme	Shelf	66:70	arg1	Life					72:75	the Shelf Life	62:75	the Shelf Life of Bod Ljong Cheese	62:95	Evaluation of Chitosan-Starch-Based Edible Coating To Improve the Shelf Life of Bod Ljong Cheese.
26197284	3	46	theme	water	552:556	arg1	loss					558:561	water loss	552:561	water loss	552:561	Application of coating on cheese decreased water loss, lipid oxidation, changes in headspace gas composition, and color.
26197284	3	47	from	oxidation	570:578	arg1	composition					606:616	headspace gas composition	592:616	headspace gas composition	592:616	Application of coating on cheese decreased water loss, lipid oxidation, changes in headspace gas composition, and color.
26197284	1	48	theme	ljong	222:226	arg1	cheese					228:233	Bod ljong cheese	218:233	Bod ljong cheese	218:233	The objective of this work was to evaluate the effectiveness of antimicrobial edible coatings to improve the quality of Bod ljong cheese throughout 25 days of storage.
24556118	1	0	theme	fruit	166:170	arg1	extract					172:178	Lagenaria siceraria (LS) fruit extract	141:178	Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler	141:264	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	7	1	theme	fruit	900:904	arg1	extract					906:912	LS fruit extract	897:912	LS fruit extract	897:912	The application of this technology would improve the stability of LS fruit extract in pharmaceutical and food products.
24556118	0	2	theme	co-precipitated	87:101	arg1	starch					103:108	co-precipitated starch	87:108	co-precipitated starch	87:108	Modified release and antioxidant stable Lagenaria siceraria extract microspheres using co-precipitated starch.
24556118	1	3	theme	extract	172:178	arg1	beads					132:136	Ca-alginate hydrogel beads	111:136	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler	111:264	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	5	4	theme	micromeritic	669:680	arg1	properties					682:691	the micromeritic properties	665:691	the micromeritic properties	665:691	The incorporation of filler in hydrogel beads modified the micromeritic properties and release profile of LS fruit extract.
24556118	6	5	theme	extract	822:828	arg1	potential					805:813	antioxidant potential	793:813	antioxidant potential of the extract	793:828	It is observed that fillers have improved the stability of antioxidant potential of the extract.
24556118	5	6	theme	fruit	719:723	arg1	extract					725:731	LS fruit extract	716:731	LS fruit extract	716:731	The incorporation of filler in hydrogel beads modified the micromeritic properties and release profile of LS fruit extract.
24556118	7	7	dep	pharmaceutical	917:930	arg1	products					941:948	products	941:948	products	941:948	The application of this technology would improve the stability of LS fruit extract in pharmaceutical and food products.
24556118	5	8	theme	filler	631:636	arg1	incorporation					614:626	The incorporation	610:626	The incorporation of filler in hydrogel beads	610:654	The incorporation of filler in hydrogel beads modified the micromeritic properties and release profile of LS fruit extract.
24556118	3	9	theme	chemical	478:485	arg1	composition					487:497	The chemical composition	474:497	The chemical composition of ethanolic extract	474:518	The chemical composition of ethanolic extract was analysed by LC-MS.
24556118	6	10	theme	potential	805:813	arg1	stability					780:788	the stability	776:788	the stability of antioxidant potential of the extract	776:828	It is observed that fillers have improved the stability of antioxidant potential of the extract.
24556118	4	11	theme	prepared	547:554	arg1	microspheres					556:567	The prepared microspheres	543:567	The prepared microspheres	543:567	The prepared microspheres were characterized by SEM, FTIR and XRD.
24556118	7	12	theme	technology	855:864	arg1	application					835:845	The application	831:845	The application of this technology	831:864	The application of this technology would improve the stability of LS fruit extract in pharmaceutical and food products.
24556118	5	13	theme	hydrogel	641:648	arg1	beads					650:654	hydrogel beads	641:654	hydrogel beads	641:654	The incorporation of filler in hydrogel beads modified the micromeritic properties and release profile of LS fruit extract.
24556118	6	14	theme	antioxidant	793:803	arg1	potential					805:813	antioxidant potential	793:813	antioxidant potential of the extract	793:828	It is observed that fillers have improved the stability of antioxidant potential of the extract.
24556118	2	15	theme	SSD	317:319	arg1	concentrations					299:312	different concentrations	289:312	different concentrations of SSD	289:319	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	16	theme	moisture	376:383	arg1	content					385:391	moisture content	376:391	moisture content	376:391	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	1	17	theme	Ca-alginate	111:121	arg1	beads					132:136	Ca-alginate hydrogel beads	111:136	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler	111:264	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	1	18	theme	LS	205:206	arg1	starch					213:218	LS seed starch	205:218	LS seed starch	205:218	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	0	19	theme	antioxidant	21:31	arg1	microspheres					68:79	antioxidant stable Lagenaria siceraria extract microspheres	21:79	antioxidant stable Lagenaria siceraria extract microspheres using co-precipitated starch	21:108	Modified release and antioxidant stable Lagenaria siceraria extract microspheres using co-precipitated starch.
24556118	5	20	theme	LS	716:717	arg1	extract					725:731	LS fruit extract	716:731	LS fruit extract	716:731	The incorporation of filler in hydrogel beads modified the micromeritic properties and release profile of LS fruit extract.
24556118	2	21	theme	concentrations	299:312	arg1	Effect					279:284	Effect	279:284	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract	279:456	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	1	22	theme	seed	208:211	arg1	starch					213:218	LS seed starch	205:218	LS seed starch	205:218	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	2	23	theme	microspheres	426:437	arg1	content					385:391	moisture content	376:391	moisture content	376:391	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	23	theme	microspheres	426:437	arg1	size					354:357	size	354:357	size of microspheres	354:373	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	23	theme	microspheres	426:437	arg1	efficiency					342:351	encapsulation efficiency	328:351	encapsulation efficiency	328:351	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	23	theme	microspheres	426:437	arg1	potential					409:417	antioxidant potential	397:417	antioxidant potential	397:417	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	24	theme	microspheres	362:373	arg1	content					385:391	moisture content	376:391	moisture content	376:391	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	24	theme	microspheres	362:373	arg1	size					354:357	size	354:357	size of microspheres	354:373	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	24	theme	microspheres	362:373	arg1	efficiency					342:351	encapsulation efficiency	328:351	encapsulation efficiency	328:351	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	24	theme	microspheres	362:373	arg1	potential					409:417	antioxidant potential	397:417	antioxidant potential	397:417	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	25	theme	different	289:297	arg1	concentrations					299:312	different concentrations	289:312	different concentrations of SSD	289:319	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	1	26	theme	starch	213:218	arg1	co-precipitates					186:200	co-precipitates	186:200	co-precipitates of LS seed starch and colloidal silicon dioxide (SSD)	186:254	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	0	27	theme	Lagenaria	40:48	arg1	microspheres					68:79	antioxidant stable Lagenaria siceraria extract microspheres	21:79	antioxidant stable Lagenaria siceraria extract microspheres using co-precipitated starch	21:108	Modified release and antioxidant stable Lagenaria siceraria extract microspheres using co-precipitated starch.
24556118	2	28	theme	encapsulation	328:340	arg1	efficiency					342:351	encapsulation efficiency	328:351	encapsulation efficiency	328:351	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	5	29	theme	extract	725:731	arg1	profile					705:711	release profile	697:711	release profile	697:711	The incorporation of filler in hydrogel beads modified the micromeritic properties and release profile of LS fruit extract.
24556118	5	29	theme	extract	725:731	arg1	properties					682:691	the micromeritic properties	665:691	the micromeritic properties	665:691	The incorporation of filler in hydrogel beads modified the micromeritic properties and release profile of LS fruit extract.
24556118	7	30	theme	LS	897:898	arg1	extract					906:912	LS fruit extract	897:912	LS fruit extract	897:912	The application of this technology would improve the stability of LS fruit extract in pharmaceutical and food products.
24556118	0	31	theme	stable	33:38	arg1	microspheres					68:79	antioxidant stable Lagenaria siceraria extract microspheres	21:79	antioxidant stable Lagenaria siceraria extract microspheres using co-precipitated starch	21:108	Modified release and antioxidant stable Lagenaria siceraria extract microspheres using co-precipitated starch.
24556118	1	32	theme	colloidal	224:232	arg1	SSD					251:253	SSD	251:253	SSD	251:253	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	1	32	theme	colloidal	224:232	arg1	dioxide					242:248	colloidal silicon dioxide	224:248	colloidal silicon dioxide (SSD)	224:254	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	0	33	theme	extract	60:66	arg1	microspheres					68:79	antioxidant stable Lagenaria siceraria extract microspheres	21:79	antioxidant stable Lagenaria siceraria extract microspheres using co-precipitated starch	21:108	Modified release and antioxidant stable Lagenaria siceraria extract microspheres using co-precipitated starch.
24556118	3	34	theme	ethanolic	502:510	arg1	extract					512:518	ethanolic extract	502:518	ethanolic extract	502:518	The chemical composition of ethanolic extract was analysed by LC-MS.
24556118	0	35	theme	siceraria	50:58	arg1	microspheres					68:79	antioxidant stable Lagenaria siceraria extract microspheres	21:79	antioxidant stable Lagenaria siceraria extract microspheres using co-precipitated starch	21:108	Modified release and antioxidant stable Lagenaria siceraria extract microspheres using co-precipitated starch.
24556118	5	36	theme	release	697:703	arg1	profile					705:711	release profile	697:711	release profile	697:711	The incorporation of filler in hydrogel beads modified the micromeritic properties and release profile of LS fruit extract.
24556118	1	37	theme	Lagenaria	141:149	arg1	extract					172:178	Lagenaria siceraria (LS) fruit extract	141:178	Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler	141:264	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	2	38	dep	efficiency	342:351	arg1	the					324:326	the	324:326	the	324:326	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	1	39	theme	siceraria	151:159	arg1	extract					172:178	Lagenaria siceraria (LS) fruit extract	141:178	Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler	141:264	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	1	40	theme	hydrogel	123:130	arg1	beads					132:136	Ca-alginate hydrogel beads	111:136	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler	111:264	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	1	41	theme	silicon	234:240	arg1	SSD					251:253	SSD	251:253	SSD	251:253	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	1	41	theme	silicon	234:240	arg1	dioxide					242:248	colloidal silicon dioxide	224:248	colloidal silicon dioxide (SSD)	224:254	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	7	42	theme	extract	906:912	arg1	stability					884:892	the stability	880:892	the stability of LS fruit extract	880:912	The application of this technology would improve the stability of LS fruit extract in pharmaceutical and food products.
24556118	1	43	theme	dioxide	242:248	arg1	co-precipitates					186:200	co-precipitates	186:200	co-precipitates of LS seed starch and colloidal silicon dioxide (SSD)	186:254	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	2	44	theme	antioxidant	397:407	arg1	potential					409:417	antioxidant potential	397:417	antioxidant potential	397:417	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	5	45	from	incorporation	614:626	arg1	beads					650:654	hydrogel beads	641:654	hydrogel beads	641:654	The incorporation of filler in hydrogel beads modified the micromeritic properties and release profile of LS fruit extract.
24556118	1	46	theme	LS	162:163	arg1	extract					172:178	Lagenaria siceraria (LS) fruit extract	141:178	Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler	141:264	Ca-alginate hydrogel beads of Lagenaria siceraria (LS) fruit extract using co-precipitates of LS seed starch and colloidal silicon dioxide (SSD) as filler was studied.
24556118	2	47	from	Effect	279:284	arg1	content					385:391	moisture content	376:391	moisture content	376:391	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	47	from	Effect	279:284	arg1	potential					409:417	antioxidant potential	397:417	antioxidant potential	397:417	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	47	from	Effect	279:284	arg1	efficiency					342:351	encapsulation efficiency	328:351	encapsulation efficiency	328:351	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	2	47	from	Effect	279:284	arg1	size					354:357	size	354:357	size of microspheres	354:373	Effect of different concentrations of SSD on the encapsulation efficiency, size of microspheres, moisture content and antioxidant potential of the microspheres comprising extract was determined.
24556118	3	48	theme	extract	512:518	arg1	composition					487:497	The chemical composition	474:497	The chemical composition of ethanolic extract	474:518	The chemical composition of ethanolic extract was analysed by LC-MS.
24837948	4	0	theme	TEAC	498:501	arg1	values					503:508	TEAC values	498:508	TEAC values	498:508	A subsequent dehydration increased TEAC values, resistant starch content and decreased starch digestibility.
24837948	6	1	theme	highest	732:738	arg1	content					740:746	the highest content	728:746	the highest content of most of the compounds assessed	728:780	Variety had a strong impact on phytochemical profile, being Negro 8025 that exhibited the highest content of most of the compounds assessed.
24837948	1	2	theme	bioactive	150:158	arg1	composition					160:170	bioactive composition	150:170	bioactive composition	150:170	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	4	3	theme	resistant	511:519	arg1	content					528:534	resistant starch content	511:534	resistant starch content	511:534	A subsequent dehydration increased TEAC values, resistant starch content and decreased starch digestibility.
24837948	8	4	theme	pick	965:968	arg1	up					970:971	low oil pick up	957:971	low oil pick up	957:971	Dehydration produced flours with stable WAI and low oil pick up.
24837948	8	5	theme	low	957:959	arg1	up					970:971	low oil pick up	957:971	low oil pick up	957:971	Dehydration produced flours with stable WAI and low oil pick up.
24837948	4	6	theme	subsequent	465:474	arg1	dehydration					476:486	A subsequent dehydration	463:486	A subsequent dehydration	463:486	A subsequent dehydration increased TEAC values, resistant starch content and decreased starch digestibility.
24837948	9	7	theme	Negro	1013:1017	arg1	beans					1024:1028	Negro 8025 beans	1013:1028	Negro 8025 beans	1013:1028	The results suggest that the flours of Negro 8025 beans have a good potential to be considered as functional ingredient for healthy food products.
24837948	3	8	theme	phenolic	418:425	arg1	compounds					427:435	some phenolic compounds	413:435	some phenolic compounds	413:435	Cooking processes resulted in decreased values of some phenolic compounds and antioxidant capacity.
24837948	0	9	theme	dehydrated	42:51	arg1	vulgaris					63:70	dehydrated Phaseolus vulgaris	42:70	dehydrated Phaseolus vulgaris	42:70	Functional and technological potential of dehydrated Phaseolus vulgaris L. flours.
24837948	3	10	theme	compounds	427:435	arg1	values					403:408	decreased values	393:408	decreased values of some phenolic compounds and antioxidant capacity	393:460	Cooking processes resulted in decreased values of some phenolic compounds and antioxidant capacity.
24837948	3	11	theme	Cooking	363:369	arg1	processes					371:379	Cooking processes	363:379	Cooking processes	363:379	Cooking processes resulted in decreased values of some phenolic compounds and antioxidant capacity.
24837948	0	12	theme	Functional	0:9	arg1	potential					29:37	Functional and technological potential	0:37	Functional and technological potential of dehydrated Phaseolus vulgaris	0:70	Functional and technological potential of dehydrated Phaseolus vulgaris L. flours.
24837948	1	13	dep	composition	160:170	arg1	the					146:148	the	146:148	the	146:148	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	6	14	theme	Negro	702:706	arg1	8025					708:711	Negro 8025	702:711	Negro 8025 that exhibited the highest content of most of the compounds assessed	702:780	Variety had a strong impact on phytochemical profile, being Negro 8025 that exhibited the highest content of most of the compounds assessed.
24837948	8	15	with	flours	930:935	arg1	up					970:971	low oil pick up	957:971	low oil pick up	957:971	Dehydration produced flours with stable WAI and low oil pick up.
24837948	8	15	with	flours	930:935	arg1	WAI					949:951	stable WAI	942:951	stable WAI	942:951	Dehydration produced flours with stable WAI and low oil pick up.
24837948	1	16	theme	common	272:277	arg1	beans					279:283	common beans	272:283	common beans	272:283	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	1	17	theme	cooking	97:103	arg1	effect					87:92	The effect	83:92	The effect of cooking followed by dehydration	83:127	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	9	18	theme	functional	1072:1081	arg1	ingredient					1083:1092	functional ingredient	1072:1092	functional ingredient for healthy food products	1072:1118	The results suggest that the flours of Negro 8025 beans have a good potential to be considered as functional ingredient for healthy food products.
24837948	3	19	theme	capacity	453:460	arg1	values					403:408	decreased values	393:408	decreased values of some phenolic compounds and antioxidant capacity	393:460	Cooking processes resulted in decreased values of some phenolic compounds and antioxidant capacity.
24837948	0	20	theme	technological	15:27	arg1	potential					29:37	Functional and technological potential	0:37	Functional and technological potential of dehydrated Phaseolus vulgaris	0:70	Functional and technological potential of dehydrated Phaseolus vulgaris L. flours.
24837948	1	21	theme	beans	279:283	arg1	beans					279:283	common beans	272:283	common beans	272:283	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	1	21	theme	beans	279:283	arg1	Negro					241:245	Negro	241:245	Negro	241:245	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	1	21	theme	beans	279:283	arg1	varieties					230:238	two varieties	226:238	two varieties (Negro 8025 and Bayo Madero) of common beans	226:283	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	1	21	theme	beans	279:283	arg1	Madero					261:266	Madero	261:266	Madero	261:266	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	7	22	theme	absorption	820:829	arg1	capacity					831:838	oil absorption capacity	816:838	oil absorption capacity (OAC)	816:844	Water absorption index (WAI) and oil absorption capacity (OAC) were determined in order to measure technological suitability.
24837948	7	22	theme	absorption	820:829	arg1	OAC					841:843	OAC	841:843	OAC	841:843	Water absorption index (WAI) and oil absorption capacity (OAC) were determined in order to measure technological suitability.
24837948	7	23	theme	oil	816:818	arg1	capacity					831:838	oil absorption capacity	816:838	oil absorption capacity (OAC)	816:844	Water absorption index (WAI) and oil absorption capacity (OAC) were determined in order to measure technological suitability.
24837948	7	23	theme	oil	816:818	arg1	OAC					841:843	OAC	841:843	OAC	841:843	Water absorption index (WAI) and oil absorption capacity (OAC) were determined in order to measure technological suitability.
24837948	3	24	theme	decreased	393:401	arg1	values					403:408	decreased values	393:408	decreased values of some phenolic compounds and antioxidant capacity	393:460	Cooking processes resulted in decreased values of some phenolic compounds and antioxidant capacity.
24837948	6	25	theme	strong	656:661	arg1	impact					663:668	a strong impact	654:668	a strong impact	654:668	Variety had a strong impact on phytochemical profile, being Negro 8025 that exhibited the highest content of most of the compounds assessed.
24837948	7	26	theme	absorption	789:798	arg1	WAI					807:809	WAI	807:809	WAI	807:809	Water absorption index (WAI) and oil absorption capacity (OAC) were determined in order to measure technological suitability.
24837948	7	26	theme	absorption	789:798	arg1	index					800:804	Water absorption index	783:804	Water absorption index (WAI)	783:810	Water absorption index (WAI) and oil absorption capacity (OAC) were determined in order to measure technological suitability.
24837948	1	27	dep	varieties	230:238	arg1	Negro					241:245	Negro	241:245	Negro	241:245	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	1	27	dep	varieties	230:238	arg1	beans					279:283	common beans	272:283	common beans	272:283	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	1	27	dep	varieties	230:238	arg1	varieties					230:238	two varieties	226:238	two varieties (Negro 8025 and Bayo Madero) of common beans	226:283	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	1	27	dep	varieties	230:238	arg1	Madero					261:266	Madero	261:266	Madero	261:266	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	9	28	theme	healthy	1098:1104	arg1	products					1111:1118	healthy food products	1098:1118	healthy food products	1098:1118	The results suggest that the flours of Negro 8025 beans have a good potential to be considered as functional ingredient for healthy food products.
24837948	3	29	theme	antioxidant	441:451	arg1	capacity					453:460	antioxidant capacity	441:460	antioxidant capacity	441:460	Cooking processes resulted in decreased values of some phenolic compounds and antioxidant capacity.
24837948	0	30	theme	vulgaris	63:70	arg1	potential					29:37	Functional and technological potential	0:37	Functional and technological potential of dehydrated Phaseolus vulgaris	0:70	Functional and technological potential of dehydrated Phaseolus vulgaris L. flours.
24837948	4	31	theme	starch	521:526	arg1	content					528:534	resistant starch content	511:534	resistant starch content	511:534	A subsequent dehydration increased TEAC values, resistant starch content and decreased starch digestibility.
24837948	4	32	theme	starch	550:555	arg1	digestibility					557:569	starch digestibility	550:569	starch digestibility	550:569	A subsequent dehydration increased TEAC values, resistant starch content and decreased starch digestibility.
24837948	9	33	theme	food	1106:1109	arg1	products					1111:1118	healthy food products	1098:1118	healthy food products	1098:1118	The results suggest that the flours of Negro 8025 beans have a good potential to be considered as functional ingredient for healthy food products.
24837948	0	34	theme	Phaseolus	53:61	arg1	vulgaris					63:70	dehydrated Phaseolus vulgaris	42:70	dehydrated Phaseolus vulgaris	42:70	Functional and technological potential of dehydrated Phaseolus vulgaris L. flours.
24837948	8	35	theme	stable	942:947	arg1	WAI					949:951	stable WAI	942:951	stable WAI	942:951	Dehydration produced flours with stable WAI and low oil pick up.
24837948	6	36	theme	phytochemical	673:685	arg1	profile					687:693	phytochemical profile	673:693	phytochemical profile	673:693	Variety had a strong impact on phytochemical profile, being Negro 8025 that exhibited the highest content of most of the compounds assessed.
24837948	7	37	theme	Water	783:787	arg1	WAI					807:809	WAI	807:809	WAI	807:809	Water absorption index (WAI) and oil absorption capacity (OAC) were determined in order to measure technological suitability.
24837948	7	37	theme	Water	783:787	arg1	index					800:804	Water absorption index	783:804	Water absorption index (WAI)	783:810	Water absorption index (WAI) and oil absorption capacity (OAC) were determined in order to measure technological suitability.
24837948	6	38	contain	had	650:652	arg2	impact					663:668	a strong impact	654:668	a strong impact	654:668	Variety had a strong impact on phytochemical profile, being Negro 8025 that exhibited the highest content of most of the compounds assessed.
24837948	6	38	contain	had	650:652	arg1	Variety					642:648	Variety	642:648	Variety	642:648	Variety had a strong impact on phytochemical profile, being Negro 8025 that exhibited the highest content of most of the compounds assessed.
24837948	2	39	theme	phenolic	308:315	arg1	acids					317:321	phenolic acids	308:321	phenolic acids	308:321	Quercetin, rutin, and phenolic acids were the most abundant phenolics found.
24837948	2	39	theme	phenolic	308:315	arg1	rutin					297:301	rutin	297:301	rutin	297:301	Quercetin, rutin, and phenolic acids were the most abundant phenolics found.
24837948	2	39	theme	phenolic	308:315	arg1	Quercetin					286:294	Quercetin	286:294	Quercetin	286:294	Quercetin, rutin, and phenolic acids were the most abundant phenolics found.
24837948	2	39	theme	phenolic	308:315	arg1	phenolics					346:354	the most abundant phenolics	328:354	the most abundant phenolics found	328:360	Quercetin, rutin, and phenolic acids were the most abundant phenolics found.
24837948	0	40	dep	flours	75:80	arg1	potential					29:37	Functional and technological potential	0:37	Functional and technological potential of dehydrated Phaseolus vulgaris	0:70	Functional and technological potential of dehydrated Phaseolus vulgaris L. flours.
24837948	1	41	theme	varieties	230:238	arg1	activity					185:192	antioxidant activity	173:192	antioxidant activity	173:192	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	1	41	theme	varieties	230:238	arg1	properties					212:221	technological properties	198:221	technological properties	198:221	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	1	41	theme	varieties	230:238	arg1	composition					160:170	bioactive composition	150:170	bioactive composition	150:170	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	9	42	contain	have	1030:1033	arg2	potential					1042:1050	a good potential	1035:1050	a good potential to be considered as functional ingredient for healthy food products	1035:1118	The results suggest that the flours of Negro 8025 beans have a good potential to be considered as functional ingredient for healthy food products.
24837948	9	42	contain	have	1030:1033	arg1	flours					1003:1008	the flours	999:1008	the flours of Negro 8025 beans	999:1028	The results suggest that the flours of Negro 8025 beans have a good potential to be considered as functional ingredient for healthy food products.
24837948	6	43	theme	most	751:754	arg1	content					740:746	the highest content	728:746	the highest content of most of the compounds assessed	728:780	Variety had a strong impact on phytochemical profile, being Negro 8025 that exhibited the highest content of most of the compounds assessed.
24837948	7	44	theme	technological	882:894	arg1	suitability					896:906	technological suitability	882:906	technological suitability	882:906	Water absorption index (WAI) and oil absorption capacity (OAC) were determined in order to measure technological suitability.
24837948	9	45	theme	good	1037:1040	arg1	potential					1042:1050	a good potential	1035:1050	a good potential to be considered as functional ingredient for healthy food products	1035:1118	The results suggest that the flours of Negro 8025 beans have a good potential to be considered as functional ingredient for healthy food products.
24837948	1	46	theme	technological	198:210	arg1	properties					212:221	technological properties	198:221	technological properties	198:221	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	1	47	theme	antioxidant	173:183	arg1	activity					185:192	antioxidant activity	173:192	antioxidant activity	173:192	The effect of cooking followed by dehydration was evaluated on the bioactive composition, antioxidant activity and technological properties of two varieties (Negro 8025 and Bayo Madero) of common beans.
24837948	9	48	theme	8025	1019:1022	arg1	beans					1024:1028	Negro 8025 beans	1013:1028	Negro 8025 beans	1013:1028	The results suggest that the flours of Negro 8025 beans have a good potential to be considered as functional ingredient for healthy food products.
24837948	8	49	theme	oil	961:963	arg1	up					970:971	low oil pick up	957:971	low oil pick up	957:971	Dehydration produced flours with stable WAI and low oil pick up.
24837948	2	50	theme	abundant	337:344	arg1	acids					317:321	phenolic acids	308:321	phenolic acids	308:321	Quercetin, rutin, and phenolic acids were the most abundant phenolics found.
24837948	2	50	theme	abundant	337:344	arg1	rutin					297:301	rutin	297:301	rutin	297:301	Quercetin, rutin, and phenolic acids were the most abundant phenolics found.
24837948	2	50	theme	abundant	337:344	arg1	Quercetin					286:294	Quercetin	286:294	Quercetin	286:294	Quercetin, rutin, and phenolic acids were the most abundant phenolics found.
24837948	2	50	theme	abundant	337:344	arg1	phenolics					346:354	the most abundant phenolics	328:354	the most abundant phenolics found	328:360	Quercetin, rutin, and phenolic acids were the most abundant phenolics found.
24837948	9	51	theme	beans	1024:1028	arg1	flours					1003:1008	the flours	999:1008	the flours of Negro 8025 beans	999:1028	The results suggest that the flours of Negro 8025 beans have a good potential to be considered as functional ingredient for healthy food products.
28821032	0	0	theme	antibacterial	69:81	arg1	dressing					89:96	antibacterial wound dressing	69:96	antibacterial wound dressing	69:96	Using oxidized amylose as carrier of linalool for the development of antibacterial wound dressing.
28821032	10	1	theme	wound	1205:1209	arg1	healing					1211:1217	wound healing	1205:1217	wound healing applications	1205:1230	Overall, the developed antibacterial wound dressings hold great potential for use in wound healing applications.
28821032	9	2	theme	skin	1084:1087	arg1	tissue					1089:1094	regenerated skin tissue	1072:1094	regenerated skin tissue	1072:1094	It effectively prevented inflammation in regenerated skin tissue and scar formation too.
28821032	4	3	contain	possessed	526:534	arg2	structure					543:551	porous structure	536:551	porous structure	536:551	The developed composite dressings possessed porous structure.
28821032	4	3	contain	possessed	526:534	arg1	dressings					516:524	The developed composite dressings	492:524	The developed composite dressings	492:524	The developed composite dressings possessed porous structure.
28821032	8	4	theme	composite	931:939	arg1	dressing					941:948	This composite dressing	926:948	This composite dressing	926:948	This composite dressing effectively promoted granulation tissue formation and accelerated wound healing.
28821032	0	5	theme	dressing	89:96	arg1	development					54:64	the development	50:64	the development of antibacterial wound dressing	50:96	Using oxidized amylose as carrier of linalool for the development of antibacterial wound dressing.
28821032	4	6	theme	porous	536:541	arg1	structure					543:551	porous structure	536:551	porous structure	536:551	The developed composite dressings possessed porous structure.
28821032	0	7	theme	wound	83:87	arg1	dressing					89:96	antibacterial wound dressing	69:96	antibacterial wound dressing	69:96	Using oxidized amylose as carrier of linalool for the development of antibacterial wound dressing.
28821032	10	8	theme	healing	1211:1217	arg1	applications					1219:1230	wound healing applications	1205:1230	wound healing applications	1205:1230	Overall, the developed antibacterial wound dressings hold great potential for use in wound healing applications.
28821032	5	9	theme	exudates	645:652	arg1	accumulation					654:665	the excess exudates accumulation	634:665	the excess exudates accumulation	634:665	They had abilities to keep the wound in moist environment and meanwhile prevent the excess exudates accumulation.
28821032	3	10	theme	amyloses	482:489	arg1	effect					463:468	the solubilization effect	444:468	the solubilization effect of oxidized amyloses	444:489	Our results showed that the content of linalool in the composite dressings was efficiently increased thanks to the solubilization effect of oxidized amyloses.
28821032	2	11	used	used	264:267	arg2	Encapsulation					224:236	Encapsulation	224:236	Encapsulation with oxidized amylose	224:258	Encapsulation with oxidized amylose was used as an effective way to introduce linalool into collagen matrix.
28821032	2	11	used	used	264:267	arg2	way					285:287	an effective way	272:287	an effective way to introduce linalool into collagen matrix	272:330	Encapsulation with oxidized amylose was used as an effective way to introduce linalool into collagen matrix.
28821032	4	12	theme	composite	506:514	arg1	dressings					516:524	The developed composite dressings	492:524	The developed composite dressings	492:524	The developed composite dressings possessed porous structure.
28821032	2	13	theme	effective	275:283	arg1	Encapsulation					224:236	Encapsulation	224:236	Encapsulation with oxidized amylose	224:258	Encapsulation with oxidized amylose was used as an effective way to introduce linalool into collagen matrix.
28821032	2	13	theme	effective	275:283	arg1	way					285:287	an effective way	272:287	an effective way to introduce linalool into collagen matrix	272:330	Encapsulation with oxidized amylose was used as an effective way to introduce linalool into collagen matrix.
28821032	6	14	theme	antibacterial	747:759	arg1	activities					761:770	excellent antibacterial activities	737:770	excellent antibacterial activities	737:770	The incorporation of linalool conferred the composite dressings with excellent antibacterial activities as expected.
28821032	3	15	theme	solubilization	448:461	arg1	effect					463:468	the solubilization effect	444:468	the solubilization effect of oxidized amyloses	444:489	Our results showed that the content of linalool in the composite dressings was efficiently increased thanks to the solubilization effect of oxidized amyloses.
28821032	0	16	theme	oxidized	6:13	arg1	amylose					15:21	oxidized amylose	6:21	oxidized amylose	6:21	Using oxidized amylose as carrier of linalool for the development of antibacterial wound dressing.
28821032	4	17	theme	developed	496:504	arg1	dressings					516:524	The developed composite dressings	492:524	The developed composite dressings	492:524	The developed composite dressings possessed porous structure.
28821032	5	18	theme	moist	594:598	arg1	environment					600:610	moist environment	594:610	moist environment	594:610	They had abilities to keep the wound in moist environment and meanwhile prevent the excess exudates accumulation.
28821032	6	19	theme	excellent	737:745	arg1	activities					761:770	excellent antibacterial activities	737:770	excellent antibacterial activities	737:770	The incorporation of linalool conferred the composite dressings with excellent antibacterial activities as expected.
28821032	3	20	theme	composite	388:396	arg1	dressings					398:406	the composite dressings	384:406	the composite dressings	384:406	Our results showed that the content of linalool in the composite dressings was efficiently increased thanks to the solubilization effect of oxidized amyloses.
28821032	7	21	theme	enhanced	865:872	arg1	compatibility					880:892	enhanced blood compatibility	865:892	enhanced blood compatibility	865:892	Moreover, the composite dressing with the highest content of linalool presented enhanced blood compatibility and good cell biocompatibility.
28821032	10	22	theme	great	1178:1182	arg1	potential					1184:1192	great potential	1178:1192	great potential for use in wound healing applications	1178:1230	Overall, the developed antibacterial wound dressings hold great potential for use in wound healing applications.
28821032	2	23	theme	collagen	316:323	arg1	matrix					325:330	collagen matrix	316:330	collagen matrix	316:330	Encapsulation with oxidized amylose was used as an effective way to introduce linalool into collagen matrix.
28821032	7	24	theme	good	898:901	arg1	biocompatibility					908:923	good cell biocompatibility	898:923	good cell biocompatibility	898:923	Moreover, the composite dressing with the highest content of linalool presented enhanced blood compatibility and good cell biocompatibility.
28821032	1	25	theme	linalool/oxidized	179:195	arg1	complex					215:221	linalool/oxidized amylose inclusion complex	179:221	linalool/oxidized amylose inclusion complex	179:221	This study aimed to prepare antibacterial wound dressings based on collagen and linalool/oxidized amylose inclusion complex.
28821032	3	26	theme	linalool	372:379	arg1	content					361:367	the content	357:367	the content of linalool in the composite dressings	357:406	Our results showed that the content of linalool in the composite dressings was efficiently increased thanks to the solubilization effect of oxidized amyloses.
28821032	2	27	with	Encapsulation	224:236	arg1	amylose					252:258	oxidized amylose	243:258	oxidized amylose	243:258	Encapsulation with oxidized amylose was used as an effective way to introduce linalool into collagen matrix.
28821032	5	28	theme	excess	638:643	arg1	accumulation					654:665	the excess exudates accumulation	634:665	the excess exudates accumulation	634:665	They had abilities to keep the wound in moist environment and meanwhile prevent the excess exudates accumulation.
28821032	5	29	dep	abilities	563:571	arg1	keep					576:579	keep	576:579	to keep the wound in moist environment	573:610	They had abilities to keep the wound in moist environment and meanwhile prevent the excess exudates accumulation.
28821032	5	29	dep	abilities	563:571	arg1	prevent					626:632	prevent	626:632	prevent the excess exudates accumulation	626:665	They had abilities to keep the wound in moist environment and meanwhile prevent the excess exudates accumulation.
28821032	3	30	theme	oxidized	473:480	arg1	amyloses					482:489	oxidized amyloses	473:489	oxidized amyloses	473:489	Our results showed that the content of linalool in the composite dressings was efficiently increased thanks to the solubilization effect of oxidized amyloses.
28821032	7	31	theme	linalool	846:853	arg1	content					835:841	the highest content	823:841	the highest content of linalool	823:853	Moreover, the composite dressing with the highest content of linalool presented enhanced blood compatibility and good cell biocompatibility.
28821032	10	32	theme	developed	1133:1141	arg1	dressings					1163:1171	the developed antibacterial wound dressings	1129:1171	the developed antibacterial wound dressings	1129:1171	Overall, the developed antibacterial wound dressings hold great potential for use in wound healing applications.
28821032	6	33	theme	composite	712:720	arg1	dressings					722:730	the composite dressings	708:730	the composite dressings with excellent antibacterial activities	708:770	The incorporation of linalool conferred the composite dressings with excellent antibacterial activities as expected.
28821032	1	34	theme	inclusion	205:213	arg1	complex					215:221	linalool/oxidized amylose inclusion complex	179:221	linalool/oxidized amylose inclusion complex	179:221	This study aimed to prepare antibacterial wound dressings based on collagen and linalool/oxidized amylose inclusion complex.
28821032	9	35	theme	scar	1100:1103	arg1	formation					1105:1113	scar formation	1100:1113	scar formation	1100:1113	It effectively prevented inflammation in regenerated skin tissue and scar formation too.
28821032	5	36	from	wound	585:589	arg1	environment					600:610	moist environment	594:610	moist environment	594:610	They had abilities to keep the wound in moist environment and meanwhile prevent the excess exudates accumulation.
28821032	10	37	from	potential	1184:1192	arg1	applications					1219:1230	wound healing applications	1205:1230	wound healing applications	1205:1230	Overall, the developed antibacterial wound dressings hold great potential for use in wound healing applications.
28821032	3	38	from	content	361:367	arg1	dressings					398:406	the composite dressings	384:406	the composite dressings	384:406	Our results showed that the content of linalool in the composite dressings was efficiently increased thanks to the solubilization effect of oxidized amyloses.
28821032	2	39	theme	oxidized	243:250	arg1	amylose					252:258	oxidized amylose	243:258	oxidized amylose	243:258	Encapsulation with oxidized amylose was used as an effective way to introduce linalool into collagen matrix.
28821032	7	40	theme	cell	903:906	arg1	biocompatibility					908:923	good cell biocompatibility	898:923	good cell biocompatibility	898:923	Moreover, the composite dressing with the highest content of linalool presented enhanced blood compatibility and good cell biocompatibility.
28821032	7	41	theme	blood	874:878	arg1	compatibility					880:892	enhanced blood compatibility	865:892	enhanced blood compatibility	865:892	Moreover, the composite dressing with the highest content of linalool presented enhanced blood compatibility and good cell biocompatibility.
28821032	8	42	theme	wound	1016:1020	arg1	healing					1022:1028	wound healing	1016:1028	wound healing	1016:1028	This composite dressing effectively promoted granulation tissue formation and accelerated wound healing.
28821032	0	43	theme	linalool	37:44	arg1	carrier					26:32	carrier	26:32	carrier of linalool for the development of antibacterial wound dressing	26:96	Using oxidized amylose as carrier of linalool for the development of antibacterial wound dressing.
28821032	7	44	theme	highest	827:833	arg1	content					835:841	the highest content	823:841	the highest content of linalool	823:853	Moreover, the composite dressing with the highest content of linalool presented enhanced blood compatibility and good cell biocompatibility.
28821032	1	45	theme	antibacterial	127:139	arg1	dressings					147:155	antibacterial wound dressings	127:155	antibacterial wound dressings based on collagen and linalool/oxidized amylose inclusion complex	127:221	This study aimed to prepare antibacterial wound dressings based on collagen and linalool/oxidized amylose inclusion complex.
28821032	6	46	theme	linalool	689:696	arg1	incorporation					672:684	The incorporation	668:684	The incorporation of linalool	668:696	The incorporation of linalool conferred the composite dressings with excellent antibacterial activities as expected.
28821032	6	47	with	dressings	722:730	arg1	activities					761:770	excellent antibacterial activities	737:770	excellent antibacterial activities	737:770	The incorporation of linalool conferred the composite dressings with excellent antibacterial activities as expected.
28821032	10	48	theme	wound	1157:1161	arg1	dressings					1163:1171	the developed antibacterial wound dressings	1129:1171	the developed antibacterial wound dressings	1129:1171	Overall, the developed antibacterial wound dressings hold great potential for use in wound healing applications.
28821032	5	49	contain	had	559:561	arg2	abilities					563:571	abilities	563:571	abilities to keep the wound in moist environment and meanwhile prevent the excess exudates accumulation	563:665	They had abilities to keep the wound in moist environment and meanwhile prevent the excess exudates accumulation.
28821032	5	49	contain	had	559:561	arg1	They					554:557	They	554:557	They	554:557	They had abilities to keep the wound in moist environment and meanwhile prevent the excess exudates accumulation.
28821032	8	50	theme	granulation	971:981	arg1	formation					990:998	granulation tissue formation	971:998	granulation tissue formation	971:998	This composite dressing effectively promoted granulation tissue formation and accelerated wound healing.
28821032	10	51	theme	antibacterial	1143:1155	arg1	dressings					1163:1171	the developed antibacterial wound dressings	1129:1171	the developed antibacterial wound dressings	1129:1171	Overall, the developed antibacterial wound dressings hold great potential for use in wound healing applications.
28821032	1	52	theme	amylose	197:203	arg1	complex					215:221	linalool/oxidized amylose inclusion complex	179:221	linalool/oxidized amylose inclusion complex	179:221	This study aimed to prepare antibacterial wound dressings based on collagen and linalool/oxidized amylose inclusion complex.
28821032	2	53	dep	way	285:287	arg1	introduce					292:300	introduce	292:300	to introduce linalool into collagen matrix	289:330	Encapsulation with oxidized amylose was used as an effective way to introduce linalool into collagen matrix.
28821032	9	54	theme	regenerated	1072:1082	arg1	tissue					1089:1094	regenerated skin tissue	1072:1094	regenerated skin tissue	1072:1094	It effectively prevented inflammation in regenerated skin tissue and scar formation too.
28821032	8	55	theme	tissue	983:988	arg1	formation					990:998	granulation tissue formation	971:998	granulation tissue formation	971:998	This composite dressing effectively promoted granulation tissue formation and accelerated wound healing.
28821032	7	56	with	dressing	809:816	arg1	content					835:841	the highest content	823:841	the highest content of linalool	823:853	Moreover, the composite dressing with the highest content of linalool presented enhanced blood compatibility and good cell biocompatibility.
28821032	1	57	theme	wound	141:145	arg1	dressings					147:155	antibacterial wound dressings	127:155	antibacterial wound dressings based on collagen and linalool/oxidized amylose inclusion complex	127:221	This study aimed to prepare antibacterial wound dressings based on collagen and linalool/oxidized amylose inclusion complex.
28821032	7	58	theme	composite	799:807	arg1	dressing					809:816	the composite dressing	795:816	the composite dressing with the highest content of linalool	795:853	Moreover, the composite dressing with the highest content of linalool presented enhanced blood compatibility and good cell biocompatibility.
25766820	2	0	theme	response	444:451	arg1	methodology					461:471	response surface methodology	444:471	response surface methodology	444:471	A central composite face centered design was used to establish the optimum conditions for clarification of pomegranate juice (PJ) using response surface methodology.
25766820	2	1	theme	composite	318:326	arg1	design					342:347	A central composite face centered design	308:347	A central composite face centered design	308:347	A central composite face centered design was used to establish the optimum conditions for clarification of pomegranate juice (PJ) using response surface methodology.
25766820	1	2	theme	pomegranate	205:215	arg1	juice					217:221	clear pomegranate juice	199:221	clear pomegranate juice	199:221	In this study, for the first time, the use of chitosan as a clarifying agent in the production of clear pomegranate juice was evaluated and its effects on quality characteristics of juice were investigated.
25766820	4	3	theme	10°C	815:818	arg1	time					807:810	time	807:810	time	807:810	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	4	3	theme	10°C	815:818	arg1	process					783:789	a process temperature and time	781:810	process	783:789	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	2	4	theme	central	310:316	arg1	design					342:347	A central composite face centered design	308:347	A central composite face centered design	308:347	A central composite face centered design was used to establish the optimum conditions for clarification of pomegranate juice (PJ) using response surface methodology.
25766820	1	5	theme	juice	217:221	arg1	production					185:194	the production	181:194	the production of clear pomegranate juice	181:221	In this study, for the first time, the use of chitosan as a clarifying agent in the production of clear pomegranate juice was evaluated and its effects on quality characteristics of juice were investigated.
25766820	5	6	theme	titratable	991:1000	arg1	characteristics					907:921	the quality characteristics	895:921	the quality characteristics such as turbidity	895:939	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	6	theme	titratable	991:1000	arg1	turbidity					931:939	turbidity	931:939	turbidity	931:939	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	6	theme	titratable	991:1000	arg1	acidity					1002:1008	titratable acidity	991:1008	titratable acidity	991:1008	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	3	7	theme	∗	639:639	arg1	values					642:647	a(∗) values	637:647	a(∗) values	637:647	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	3	8	theme	chitosan	514:521	arg1	temperature					550:560	process temperature	542:560	process temperature (10-20°C)	542:570	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	3	8	theme	chitosan	514:521	arg1	time					585:588	process time	577:588	process time (30-90 min)	577:600	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	3	8	theme	chitosan	514:521	arg1	factors					484:490	The three factors	474:490	The three factors	474:490	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	3	8	theme	chitosan	514:521	arg1	mg/100ml					531:538	10-120 mg/100ml	524:538	10-120 mg/100ml	524:538	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	3	8	theme	chitosan	514:521	arg1	10-20°C					563:569	10-20°C	563:569	10-20°C	563:569	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	3	8	theme	chitosan	514:521	arg1	concentration					497:509	concentration	497:509	concentration of chitosan (10-120 mg/100ml)	497:539	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	3	8	theme	chitosan	514:521	arg1	min					597:599	30-90 min	591:599	30-90 min	591:599	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	1	9	from	effects	245:251	arg1	characteristics					264:278	quality characteristics	256:278	quality characteristics of juice	256:287	In this study, for the first time, the use of chitosan as a clarifying agent in the production of clear pomegranate juice was evaluated and its effects on quality characteristics of juice were investigated.
25766820	2	10	theme	optimum	375:381	arg1	conditions					383:392	the optimum conditions	371:392	the optimum conditions for clarification of pomegranate juice (PJ)	371:436	A central composite face centered design was used to establish the optimum conditions for clarification of pomegranate juice (PJ) using response surface methodology.
25766820	4	11	theme	function	689:696	arg1	method					698:703	a desirability function method	674:703	a desirability function method	674:703	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	3	12	theme	a	637:637	arg1	values					642:647	a(∗) values	637:647	a(∗) values	637:647	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	4	13	dep	process	783:789	arg1	temperature					791:801	temperature	791:801	temperature	791:801	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	4	14	theme	desirability	676:687	arg1	method					698:703	a desirability function method	674:703	a desirability function method	674:703	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	2	15	theme	pomegranate	415:425	arg1	PJ					434:435	PJ	434:435	PJ	434:435	A central composite face centered design was used to establish the optimum conditions for clarification of pomegranate juice (PJ) using response surface methodology.
25766820	2	15	theme	pomegranate	415:425	arg1	juice					427:431	pomegranate juice	415:431	pomegranate juice (PJ)	415:436	A central composite face centered design was used to establish the optimum conditions for clarification of pomegranate juice (PJ) using response surface methodology.
25766820	4	16	theme	mg/100ml	760:767	arg1	conditions					726:735	the optimum process conditions	706:735	the optimum process conditions	706:735	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	4	16	theme	mg/100ml	760:767	arg1	chitosan					769:776	68.93 mg/100ml chitosan	754:776	68.93 mg/100ml chitosan	754:776	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	5	17	from	4	1104:1104	arg1	storage					1093:1099	storage	1093:1099	storage at 4 and 20°C for 6 months	1093:1126	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	1	18	theme	chitosan	147:154	arg1	use					140:142	the use	136:142	the use of chitosan as a clarifying agent in the production of clear pomegranate juice	136:221	In this study, for the first time, the use of chitosan as a clarifying agent in the production of clear pomegranate juice was evaluated and its effects on quality characteristics of juice were investigated.
25766820	4	19	theme	68.93	754:758	arg1	conditions					726:735	the optimum process conditions	706:735	the optimum process conditions	706:735	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	4	19	theme	68.93	754:758	arg1	chitosan					769:776	68.93 mg/100ml chitosan	754:776	68.93 mg/100ml chitosan	754:776	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	0	20	theme	juice	29:33	arg1	Clarification					0:12	Clarification	0:12	Clarification of pomegranate juice with chitosan:	0:48	Clarification of pomegranate juice with chitosan: changes on quality characteristics during storage.
25766820	0	21	theme	pomegranate	17:27	arg1	juice					29:33	pomegranate juice	17:33	pomegranate juice	17:33	Clarification of pomegranate juice with chitosan: changes on quality characteristics during storage.
25766820	2	22	theme	centered	333:340	arg1	design					342:347	A central composite face centered design	308:347	A central composite face centered design	308:347	A central composite face centered design was used to establish the optimum conditions for clarification of pomegranate juice (PJ) using response surface methodology.
25766820	5	23	dep	phenolic	1017:1024	arg1	monomeric					1027:1035	monomeric	1027:1035	monomeric	1027:1035	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	1	24	theme	clarifying	161:170	arg1	agent					172:176	a clarifying agent	159:176	a clarifying agent in the production of clear pomegranate juice	159:221	In this study, for the first time, the use of chitosan as a clarifying agent in the production of clear pomegranate juice was evaluated and its effects on quality characteristics of juice were investigated.
25766820	3	25	from	effects	612:618	arg1	turbidity					623:631	turbidity	623:631	turbidity	623:631	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	3	25	from	effects	612:618	arg1	values					642:647	a(∗) values	637:647	a(∗) values	637:647	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	4	26	theme	min	827:829	arg1	time					807:810	time	807:810	time	807:810	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	4	26	theme	min	827:829	arg1	process					783:789	a process temperature and time	781:810	process	783:789	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	5	27	theme	quality	899:905	arg1	acidity					1002:1008	titratable acidity	991:1008	titratable acidity	991:1008	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	27	theme	quality	899:905	arg1	characteristics					907:921	the quality characteristics	895:921	the quality characteristics such as turbidity	895:939	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	27	theme	quality	899:905	arg1	turbidity					931:939	turbidity	931:939	turbidity	931:939	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	27	theme	quality	899:905	arg1	characteristics					949:963	colour characteristics	942:963	colour characteristics (L(∗), a(∗), b(∗), C(∗))	942:988	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	27	theme	quality	899:905	arg1	anthocyanin					1037:1047	total phenolic, monomeric anthocyanin	1011:1047	total phenolic, monomeric anthocyanin	1011:1047	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	1	28	theme	quality	256:262	arg1	characteristics					264:278	quality characteristics	256:278	quality characteristics of juice	256:287	In this study, for the first time, the use of chitosan as a clarifying agent in the production of clear pomegranate juice was evaluated and its effects on quality characteristics of juice were investigated.
25766820	5	29	dep	characteristics	949:963	arg1	a					972:972	a	972:972	a	972:972	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	29	dep	characteristics	949:963	arg1	L					966:966	L(∗)	966:969	L(∗)	966:969	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	3	30	theme	process	577:583	arg1	time					585:588	process time	577:588	process time (30-90 min)	577:600	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	3	30	theme	process	577:583	arg1	min					597:599	30-90 min	591:599	30-90 min	591:599	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	4	31	theme	process	718:724	arg1	conditions					726:735	the optimum process conditions	706:735	the optimum process conditions	706:735	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	4	31	theme	process	718:724	arg1	chitosan					769:776	68.93 mg/100ml chitosan	754:776	68.93 mg/100ml chitosan	754:776	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	1	32	from	agent	172:176	arg1	production					185:194	the production	181:194	the production of clear pomegranate juice	181:221	In this study, for the first time, the use of chitosan as a clarifying agent in the production of clear pomegranate juice was evaluated and its effects on quality characteristics of juice were investigated.
25766820	2	33	theme	juice	427:431	arg1	clarification					398:410	clarification	398:410	clarification of pomegranate juice (PJ)	398:436	A central composite face centered design was used to establish the optimum conditions for clarification of pomegranate juice (PJ) using response surface methodology.
25766820	3	34	theme	process	542:548	arg1	temperature					550:560	process temperature	542:560	process temperature (10-20°C)	542:570	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	3	34	theme	process	542:548	arg1	10-20°C					563:569	10-20°C	563:569	10-20°C	563:569	The three factors were concentration of chitosan (10-120 mg/100ml), process temperature (10-20°C), and process time (30-90 min) and their effects on turbidity and a(∗) values were investigated.
25766820	5	35	theme	protein	1054:1060	arg1	contents					1062:1069	protein contents	1054:1069	protein contents	1054:1069	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	1	36	theme	juice	283:287	arg1	characteristics					264:278	quality characteristics	256:278	quality characteristics of juice	256:287	In this study, for the first time, the use of chitosan as a clarifying agent in the production of clear pomegranate juice was evaluated and its effects on quality characteristics of juice were investigated.
25766820	4	37	from	time	807:810	arg1	conditions					726:735	the optimum process conditions	706:735	the optimum process conditions	706:735	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	4	37	from	time	807:810	arg1	chitosan					769:776	68.93 mg/100ml chitosan	754:776	68.93 mg/100ml chitosan	754:776	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	5	38	theme	optimum	872:878	arg1	turbidity					931:939	turbidity	931:939	turbidity	931:939	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	38	theme	optimum	872:878	arg1	conditions					880:889	the optimum conditions	868:889	the optimum conditions	868:889	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	0	39	with	Clarification	0:12	arg1	chitosan					40:47	chitosan	40:47	chitosan	40:47	Clarification of pomegranate juice with chitosan: changes on quality characteristics during storage.
25766820	5	40	theme	colour	942:947	arg1	characteristics					907:921	the quality characteristics	895:921	the quality characteristics such as turbidity	895:939	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	40	theme	colour	942:947	arg1	turbidity					931:939	turbidity	931:939	turbidity	931:939	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	40	theme	colour	942:947	arg1	characteristics					949:963	colour characteristics	942:963	colour characteristics (L(∗), a(∗), b(∗), C(∗))	942:988	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	41	theme	total	1011:1015	arg1	characteristics					907:921	the quality characteristics	895:921	the quality characteristics such as turbidity	895:939	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	41	theme	total	1011:1015	arg1	turbidity					931:939	turbidity	931:939	turbidity	931:939	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	41	theme	total	1011:1015	arg1	anthocyanin					1037:1047	total phenolic, monomeric anthocyanin	1011:1047	total phenolic, monomeric anthocyanin	1011:1047	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	0	42	theme	quality	61:67	arg1	characteristics					69:83	quality characteristics	61:83	quality characteristics	61:83	Clarification of pomegranate juice with chitosan: changes on quality characteristics during storage.
25766820	4	43	theme	optimum	710:716	arg1	conditions					726:735	the optimum process conditions	706:735	the optimum process conditions	706:735	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	4	43	theme	optimum	710:716	arg1	chitosan					769:776	68.93 mg/100ml chitosan	754:776	68.93 mg/100ml chitosan	754:776	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	1	44	theme	first	124:128	arg1	time					130:133	the first time	120:133	the first time	120:133	In this study, for the first time, the use of chitosan as a clarifying agent in the production of clear pomegranate juice was evaluated and its effects on quality characteristics of juice were investigated.
25766820	5	45	theme	phenolic	1017:1024	arg1	characteristics					907:921	the quality characteristics	895:921	the quality characteristics such as turbidity	895:939	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	45	theme	phenolic	1017:1024	arg1	turbidity					931:939	turbidity	931:939	turbidity	931:939	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	5	45	theme	phenolic	1017:1024	arg1	anthocyanin					1037:1047	total phenolic, monomeric anthocyanin	1011:1047	total phenolic, monomeric anthocyanin	1011:1047	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	2	46	theme	surface	453:459	arg1	methodology					461:471	response surface methodology	444:471	response surface methodology	444:471	A central composite face centered design was used to establish the optimum conditions for clarification of pomegranate juice (PJ) using response surface methodology.
25766820	4	47	from	process	783:789	arg1	conditions					726:735	the optimum process conditions	706:735	the optimum process conditions	706:735	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	4	47	from	process	783:789	arg1	chitosan					769:776	68.93 mg/100ml chitosan	754:776	68.93 mg/100ml chitosan	754:776	Using a desirability function method, the optimum process conditions were found to be 68.93 mg/100ml chitosan at a process temperature and time of 10°C and 30 min, respectively.
25766820	5	48	from	20°C	1110:1113	arg1	storage					1093:1099	storage	1093:1099	storage at 4 and 20°C for 6 months	1093:1126	PJ was produced using the optimum conditions and the quality characteristics such as turbidity, colour characteristics (L(∗), a(∗), b(∗), C(∗)), titratable acidity, total phenolic, monomeric anthocyanin, and protein contents were evaluated during storage at 4 and 20°C for 6 months.
25766820	2	49	used	used	353:356	arg2	design					342:347	A central composite face centered design	308:347	A central composite face centered design	308:347	A central composite face centered design was used to establish the optimum conditions for clarification of pomegranate juice (PJ) using response surface methodology.
25766820	2	50	theme	face	328:331	arg1	design					342:347	A central composite face centered design	308:347	A central composite face centered design	308:347	A central composite face centered design was used to establish the optimum conditions for clarification of pomegranate juice (PJ) using response surface methodology.
25766820	1	51	theme	clear	199:203	arg1	juice					217:221	clear pomegranate juice	199:221	clear pomegranate juice	199:221	In this study, for the first time, the use of chitosan as a clarifying agent in the production of clear pomegranate juice was evaluated and its effects on quality characteristics of juice were investigated.
27185131	0	0	theme	alginate	95:102	arg1	nanocomposites					104:117	TiO2/Sodium alginate nanocomposites	83:117	TiO2/Sodium alginate nanocomposites	83:117	Photocatalytic activity and antimicrobial properties of paper sheets modified with TiO2/Sodium alginate nanocomposites.
27185131	3	1	theme	scanning	329:336	arg1	microscope					347:356	scanning electron microscope	329:356	scanning electron microscope (SEM)	329:362	Their morphology was studied by scanning electron microscope (SEM) and energy dispersive X-ray (EDX).
27185131	3	1	theme	scanning	329:336	arg1	SEM					359:361	SEM	359:361	SEM	359:361	Their morphology was studied by scanning electron microscope (SEM) and energy dispersive X-ray (EDX).
27185131	9	2	dep	collapse	1302:1309	arg1	composite					1292:1300	composite	1292:1300	composite	1292:1300	Moreover, it was found that by adding 20% TSA to the paper matrix, the properties of the paper composite collapse.
27185131	1	3	theme	different	177:185	arg1	ratios					187:192	different ratios	177:192	different ratios of TiO2/Sodium alginate (TSA) nanocomposite	177:236	Photocatalytic paper sheets were prepared by addition of different ratios of TiO2/Sodium alginate (TSA) nanocomposite.
27185131	8	4	theme	15	1188:1189	arg1	%					1190:1190	%	1190:1190	%	1190:1190	The maximum antimicrobial effect was found in case of specimen loaded with 15% TSA.
27185131	0	5	theme	TiO2/Sodium	83:93	arg1	nanocomposites					104:117	TiO2/Sodium alginate nanocomposites	83:117	TiO2/Sodium alginate nanocomposites	83:117	Photocatalytic activity and antimicrobial properties of paper sheets modified with TiO2/Sodium alginate nanocomposites.
27185131	1	6	theme	ratios	187:192	arg1	addition					165:172	addition	165:172	addition of different ratios of TiO2/Sodium alginate (TSA) nanocomposite	165:236	Photocatalytic paper sheets were prepared by addition of different ratios of TiO2/Sodium alginate (TSA) nanocomposite.
27185131	6	7	theme	paper	810:814	arg1	fibers					816:821	paper fibers	810:821	paper fibers	810:821	Moreover, the results also confirmed that presence of sodium alginate as biopolymer increased adhesion of nanoparticles to paper fibers and reduced the harmful effect of the photocatalyst on them.
27185131	7	8	theme	Candida	1095:1101	arg1	albicans					1103:1110	Candida albicans	1095:1110	Candida albicans	1095:1110	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	6	9	theme	nanoparticles	793:805	arg1	adhesion					781:788	adhesion	781:788	adhesion of nanoparticles to paper fibers	781:821	Moreover, the results also confirmed that presence of sodium alginate as biopolymer increased adhesion of nanoparticles to paper fibers and reduced the harmful effect of the photocatalyst on them.
27185131	3	10	theme	energy	368:373	arg1	EDX					393:395	EDX	393:395	EDX	393:395	Their morphology was studied by scanning electron microscope (SEM) and energy dispersive X-ray (EDX).
27185131	3	10	theme	energy	368:373	arg1	X-ray					386:390	energy dispersive X-ray	368:390	energy dispersive X-ray (EDX)	368:396	Their morphology was studied by scanning electron microscope (SEM) and energy dispersive X-ray (EDX).
27185131	4	11	theme	chemical	473:480	arg1	COD					497:499	COD	497:499	COD	497:499	Photocatalytic activity of modified paper has been studied by analysis of chemical oxygen demand (COD) of waste-water.
27185131	4	11	theme	chemical	473:480	arg1	demand					489:494	chemical oxygen demand	473:494	chemical oxygen demand (COD) of waste-water	473:515	Photocatalytic activity of modified paper has been studied by analysis of chemical oxygen demand (COD) of waste-water.
27185131	10	12	theme	modified	1373:1380	arg1	paper					1382:1386	the modified paper	1369:1386	the modified paper	1369:1386	The obtained results confirm the possible utilization of the modified paper in both hygienic and food packaging applications.
27185131	5	13	theme	chemical	602:609	arg1	COD					626:628	COD	626:628	COD	626:628	The results confirmed the mineralization of the waste-water and enhanced removal of chemical oxygen demand (COD) by increasing the amount of photocatalyst in the paper.
27185131	5	13	theme	chemical	602:609	arg1	demand					618:623	chemical oxygen demand	602:623	chemical oxygen demand (COD)	602:629	The results confirmed the mineralization of the waste-water and enhanced removal of chemical oxygen demand (COD) by increasing the amount of photocatalyst in the paper.
27185131	5	14	theme	demand	618:623	arg1	removal					591:597	removal	591:597	removal of chemical oxygen demand (COD)	591:629	The results confirmed the mineralization of the waste-water and enhanced removal of chemical oxygen demand (COD) by increasing the amount of photocatalyst in the paper.
27185131	4	15	theme	Photocatalytic	399:412	arg1	activity					414:421	Photocatalytic activity	399:421	Photocatalytic activity of modified paper	399:439	Photocatalytic activity of modified paper has been studied by analysis of chemical oxygen demand (COD) of waste-water.
27185131	4	16	theme	demand	489:494	arg1	analysis					461:468	analysis	461:468	analysis of chemical oxygen demand (COD) of waste-water	461:515	Photocatalytic activity of modified paper has been studied by analysis of chemical oxygen demand (COD) of waste-water.
27185131	9	17	theme	20	1235:1236	arg1	%					1237:1237	%	1237:1237	%	1237:1237	Moreover, it was found that by adding 20% TSA to the paper matrix, the properties of the paper composite collapse.
27185131	7	18	contain	containing	901:910	arg2	%					928:928	15%	926:928	7% as well as 15% TSA	912:932	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	7	18	contain	containing	901:910	arg2	%					913:913	7%	912:913	7% as well as 15% TSA	912:932	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	7	18	contain	containing	901:910	arg1	sheets					894:899	The paper sheets	884:899	The paper sheets containing 7% as well as 15% TSA	884:932	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	1	19	theme	TiO2/Sodium	197:207	arg1	nanocomposite					224:236	TiO2/Sodium alginate (TSA) nanocomposite	197:236	TiO2/Sodium alginate (TSA) nanocomposite	197:236	Photocatalytic paper sheets were prepared by addition of different ratios of TiO2/Sodium alginate (TSA) nanocomposite.
27185131	4	20	theme	oxygen	482:487	arg1	COD					497:499	COD	497:499	COD	497:499	Photocatalytic activity of modified paper has been studied by analysis of chemical oxygen demand (COD) of waste-water.
27185131	4	20	theme	oxygen	482:487	arg1	demand					489:494	chemical oxygen demand	473:494	chemical oxygen demand (COD) of waste-water	473:515	Photocatalytic activity of modified paper has been studied by analysis of chemical oxygen demand (COD) of waste-water.
27185131	7	21	theme	other	1066:1070	arg1	albicans					1103:1110	Candida albicans	1095:1110	Candida albicans	1095:1110	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	7	21	theme	other	1066:1070	arg1	microorganisms					1072:1085	standard antibiotic beside other microorganisms	1039:1085	standard antibiotic beside other microorganisms such as Candida albicans	1039:1110	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	5	22	theme	waste-water	566:576	arg1	mineralization					544:557	the mineralization	540:557	the mineralization of the waste-water	540:576	The results confirmed the mineralization of the waste-water and enhanced removal of chemical oxygen demand (COD) by increasing the amount of photocatalyst in the paper.
27185131	9	23	theme	paper	1250:1254	arg1	matrix					1256:1261	the paper matrix	1246:1261	the paper matrix	1246:1261	Moreover, it was found that by adding 20% TSA to the paper matrix, the properties of the paper composite collapse.
27185131	1	24	theme	alginate	209:216	arg1	nanocomposite					224:236	TiO2/Sodium alginate (TSA) nanocomposite	197:236	TiO2/Sodium alginate (TSA) nanocomposite	197:236	Photocatalytic paper sheets were prepared by addition of different ratios of TiO2/Sodium alginate (TSA) nanocomposite.
27185131	0	25	theme	Photocatalytic	0:13	arg1	activity					15:22	Photocatalytic activity	0:22	Photocatalytic activity	0:22	Photocatalytic activity and antimicrobial properties of paper sheets modified with TiO2/Sodium alginate nanocomposites.
27185131	10	26	theme	possible	1345:1352	arg1	utilization					1354:1364	the possible utilization	1341:1364	the possible utilization of the modified paper in both hygienic and food packaging applications	1341:1435	The obtained results confirm the possible utilization of the modified paper in both hygienic and food packaging applications.
27185131	0	27	theme	antimicrobial	28:40	arg1	properties					42:51	antimicrobial properties	28:51	antimicrobial properties	28:51	Photocatalytic activity and antimicrobial properties of paper sheets modified with TiO2/Sodium alginate nanocomposites.
27185131	10	28	theme	packaging	1414:1422	arg1	applications					1424:1435	both hygienic and food packaging applications	1391:1435	both hygienic and food packaging applications	1391:1435	The obtained results confirm the possible utilization of the modified paper in both hygienic and food packaging applications.
27185131	3	29	theme	dispersive	375:384	arg1	EDX					393:395	EDX	393:395	EDX	393:395	Their morphology was studied by scanning electron microscope (SEM) and energy dispersive X-ray (EDX).
27185131	3	29	theme	dispersive	375:384	arg1	X-ray					386:390	energy dispersive X-ray	368:390	energy dispersive X-ray (EDX)	368:396	Their morphology was studied by scanning electron microscope (SEM) and energy dispersive X-ray (EDX).
27185131	7	30	theme	antibiotic	1048:1057	arg1	albicans					1103:1110	Candida albicans	1095:1110	Candida albicans	1095:1110	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	7	30	theme	antibiotic	1048:1057	arg1	microorganisms					1072:1085	standard antibiotic beside other microorganisms	1039:1085	standard antibiotic beside other microorganisms such as Candida albicans	1039:1110	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	5	31	theme	oxygen	611:616	arg1	COD					626:628	COD	626:628	COD	626:628	The results confirmed the mineralization of the waste-water and enhanced removal of chemical oxygen demand (COD) by increasing the amount of photocatalyst in the paper.
27185131	5	31	theme	oxygen	611:616	arg1	demand					618:623	chemical oxygen demand	602:623	chemical oxygen demand (COD)	602:629	The results confirmed the mineralization of the waste-water and enhanced removal of chemical oxygen demand (COD) by increasing the amount of photocatalyst in the paper.
27185131	1	32	theme	Photocatalytic	120:133	arg1	sheets					141:146	Photocatalytic paper sheets	120:146	Photocatalytic paper sheets	120:146	Photocatalytic paper sheets were prepared by addition of different ratios of TiO2/Sodium alginate (TSA) nanocomposite.
27185131	10	33	theme	hygienic	1396:1403	arg1	applications					1424:1435	both hygienic and food packaging applications	1391:1435	both hygienic and food packaging applications	1391:1435	The obtained results confirm the possible utilization of the modified paper in both hygienic and food packaging applications.
27185131	1	34	theme	TSA	219:221	arg1	nanocomposite					224:236	TiO2/Sodium alginate (TSA) nanocomposite	197:236	TiO2/Sodium alginate (TSA) nanocomposite	197:236	Photocatalytic paper sheets were prepared by addition of different ratios of TiO2/Sodium alginate (TSA) nanocomposite.
27185131	6	35	from	effect	847:852	arg1	them					878:881	them	878:881	them	878:881	Moreover, the results also confirmed that presence of sodium alginate as biopolymer increased adhesion of nanoparticles to paper fibers and reduced the harmful effect of the photocatalyst on them.
27185131	7	36	theme	beside	1059:1064	arg1	albicans					1103:1110	Candida albicans	1095:1110	Candida albicans	1095:1110	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	7	36	theme	beside	1059:1064	arg1	microorganisms					1072:1085	standard antibiotic beside other microorganisms	1039:1085	standard antibiotic beside other microorganisms such as Candida albicans	1039:1110	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	1	37	theme	paper	135:139	arg1	sheets					141:146	Photocatalytic paper sheets	120:146	Photocatalytic paper sheets	120:146	Photocatalytic paper sheets were prepared by addition of different ratios of TiO2/Sodium alginate (TSA) nanocomposite.
27185131	9	38	theme	paper	1286:1290	arg1	properties					1268:1277	the properties	1264:1277	the properties of the paper	1264:1290	Moreover, it was found that by adding 20% TSA to the paper matrix, the properties of the paper composite collapse.
27185131	1	39	theme	nanocomposite	224:236	arg1	ratios					187:192	different ratios	177:192	different ratios of TiO2/Sodium alginate (TSA) nanocomposite	177:236	Photocatalytic paper sheets were prepared by addition of different ratios of TiO2/Sodium alginate (TSA) nanocomposite.
27185131	8	40	theme	%	1190:1190	arg1	TSA					1192:1194	15% TSA	1188:1194	15% TSA	1188:1194	The maximum antimicrobial effect was found in case of specimen loaded with 15% TSA.
27185131	7	41	theme	standard	1039:1046	arg1	albicans					1103:1110	Candida albicans	1095:1110	Candida albicans	1095:1110	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	7	41	theme	standard	1039:1046	arg1	microorganisms					1072:1085	standard antibiotic beside other microorganisms	1039:1085	standard antibiotic beside other microorganisms such as Candida albicans	1039:1110	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	6	42	theme	harmful	839:845	arg1	effect					847:852	the harmful effect	835:852	the harmful effect of the photocatalyst on them	835:881	Moreover, the results also confirmed that presence of sodium alginate as biopolymer increased adhesion of nanoparticles to paper fibers and reduced the harmful effect of the photocatalyst on them.
27185131	2	43	theme	paper	252:256	arg1	sheets					258:263	The modified paper sheets	239:263	The modified paper sheets	239:263	The modified paper sheets were characterized by XRD, TGA.
27185131	6	44	theme	sodium	741:746	arg1	alginate					748:755	sodium alginate	741:755	sodium alginate	741:755	Moreover, the results also confirmed that presence of sodium alginate as biopolymer increased adhesion of nanoparticles to paper fibers and reduced the harmful effect of the photocatalyst on them.
27185131	0	45	theme	sheets	62:67	arg1	activity					15:22	Photocatalytic activity	0:22	Photocatalytic activity	0:22	Photocatalytic activity and antimicrobial properties of paper sheets modified with TiO2/Sodium alginate nanocomposites.
27185131	0	45	theme	sheets	62:67	arg1	properties					42:51	antimicrobial properties	28:51	antimicrobial properties	28:51	Photocatalytic activity and antimicrobial properties of paper sheets modified with TiO2/Sodium alginate nanocomposites.
27185131	4	46	theme	waste-water	505:515	arg1	COD					497:499	COD	497:499	COD	497:499	Photocatalytic activity of modified paper has been studied by analysis of chemical oxygen demand (COD) of waste-water.
27185131	4	46	theme	waste-water	505:515	arg1	demand					489:494	chemical oxygen demand	473:494	chemical oxygen demand (COD) of waste-water	473:515	Photocatalytic activity of modified paper has been studied by analysis of chemical oxygen demand (COD) of waste-water.
27185131	2	47	theme	modified	243:250	arg1	sheets					258:263	The modified paper sheets	239:263	The modified paper sheets	239:263	The modified paper sheets were characterized by XRD, TGA.
27185131	6	48	theme	photocatalyst	861:873	arg1	effect					847:852	the harmful effect	835:852	the harmful effect of the photocatalyst on them	835:881	Moreover, the results also confirmed that presence of sodium alginate as biopolymer increased adhesion of nanoparticles to paper fibers and reduced the harmful effect of the photocatalyst on them.
27185131	0	49	theme	paper	56:60	arg1	sheets					62:67	paper sheets	56:67	paper sheets	56:67	Photocatalytic activity and antimicrobial properties of paper sheets modified with TiO2/Sodium alginate nanocomposites.
27185131	5	50	theme	photocatalyst	659:671	arg1	photocatalyst					659:671	photocatalyst	659:671	photocatalyst	659:671	The results confirmed the mineralization of the waste-water and enhanced removal of chemical oxygen demand (COD) by increasing the amount of photocatalyst in the paper.
27185131	5	50	theme	photocatalyst	659:671	arg1	amount					649:654	the amount	645:654	the amount of photocatalyst	645:671	The results confirmed the mineralization of the waste-water and enhanced removal of chemical oxygen demand (COD) by increasing the amount of photocatalyst in the paper.
27185131	4	51	theme	modified	426:433	arg1	paper					435:439	modified paper	426:439	modified paper	426:439	Photocatalytic activity of modified paper has been studied by analysis of chemical oxygen demand (COD) of waste-water.
27185131	10	52	theme	food	1409:1412	arg1	applications					1424:1435	both hygienic and food packaging applications	1391:1435	both hygienic and food packaging applications	1391:1435	The obtained results confirm the possible utilization of the modified paper in both hygienic and food packaging applications.
27185131	10	53	theme	paper	1382:1386	arg1	utilization					1354:1364	the possible utilization	1341:1364	the possible utilization of the modified paper in both hygienic and food packaging applications	1341:1435	The obtained results confirm the possible utilization of the modified paper in both hygienic and food packaging applications.
27185131	7	54	theme	paper	888:892	arg1	sheets					894:899	The paper sheets	884:899	The paper sheets containing 7% as well as 15% TSA	884:932	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	6	55	theme	alginate	748:755	arg1	presence					729:736	presence	729:736	presence of sodium alginate as biopolymer	729:769	Moreover, the results also confirmed that presence of sodium alginate as biopolymer increased adhesion of nanoparticles to paper fibers and reduced the harmful effect of the photocatalyst on them.
27185131	7	56	theme	anti-bacterial	974:987	arg1	effect					989:994	anti-bacterial effect	974:994	anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans	974:1110	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	7	57	theme	high	941:944	arg1	activity					961:968	high photocatalytic activity	941:968	high photocatalytic activity	941:968	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	8	58	theme	antimicrobial	1125:1137	arg1	effect					1139:1144	The maximum antimicrobial effect	1113:1144	The maximum antimicrobial effect	1113:1144	The maximum antimicrobial effect was found in case of specimen loaded with 15% TSA.
27185131	4	59	theme	paper	435:439	arg1	activity					414:421	Photocatalytic activity	399:421	Photocatalytic activity of modified paper	399:439	Photocatalytic activity of modified paper has been studied by analysis of chemical oxygen demand (COD) of waste-water.
27185131	3	60	theme	electron	338:345	arg1	microscope					347:356	scanning electron microscope	329:356	scanning electron microscope (SEM)	329:362	Their morphology was studied by scanning electron microscope (SEM) and energy dispersive X-ray (EDX).
27185131	3	60	theme	electron	338:345	arg1	SEM					359:361	SEM	359:361	SEM	359:361	Their morphology was studied by scanning electron microscope (SEM) and energy dispersive X-ray (EDX).
27185131	7	61	theme	photocatalytic	946:959	arg1	activity					961:968	high photocatalytic activity	941:968	high photocatalytic activity	941:968	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	10	62	from	utilization	1354:1364	arg1	applications					1424:1435	both hygienic and food packaging applications	1391:1435	both hygienic and food packaging applications	1391:1435	The obtained results confirm the possible utilization of the modified paper in both hygienic and food packaging applications.
27185131	7	63	dep	%	928:928	arg1	TSA					930:932	TSA	930:932	7% as well as 15% TSA	912:932	The paper sheets containing 7% as well as 15% TSA showed high photocatalytic activity and anti-bacterial effect against Salmonella typhimurium higher than standard antibiotic beside other microorganisms such as Candida albicans.
27185131	10	64	theme	obtained	1316:1323	arg1	results					1325:1331	The obtained results	1312:1331	The obtained results	1312:1331	The obtained results confirm the possible utilization of the modified paper in both hygienic and food packaging applications.
27185131	8	65	theme	maximum	1117:1123	arg1	effect					1139:1144	The maximum antimicrobial effect	1113:1144	The maximum antimicrobial effect	1113:1144	The maximum antimicrobial effect was found in case of specimen loaded with 15% TSA.
27185131	9	66	theme	%	1237:1237	arg1	TSA					1239:1241	20% TSA	1235:1241	20% TSA	1235:1241	Moreover, it was found that by adding 20% TSA to the paper matrix, the properties of the paper composite collapse.
27987918	5	0	contain	possessed	749:757	arg1	they					744:747	they	744:747	they	744:747	The results showed that they possessed broad spectrum of antibacterial activity.
27987918	5	0	contain	possessed	749:757	arg2	spectrum					765:772	broad spectrum	759:772	broad spectrum of antibacterial activity	759:798	The results showed that they possessed broad spectrum of antibacterial activity.
27987918	6	1	theme	viability	873:881	arg1	assay					883:887	MTT viability assay	869:887	MTT viability assay	869:887	The cytotoxicity of the prepared composite sponges was performed by MTT viability assay and fluorescence staining method on HEK293 cell lines.
27987918	1	2	theme	wound	261:265	arg1	healing					267:273	wound healing	261:273	wound healing	261:273	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges were prepared by incorporating AgSD particles into CS matrix in order to develop novel biomaterials for wound healing.
27987918	0	3	theme	wound	83:87	arg1	dressings					89:97	potential wound dressings	73:97	potential wound dressings	73:97	Construction of silver sulfadiazine loaded chitosan composite sponges as potential wound dressings.
27987918	0	3	theme	wound	83:87	arg1	sponges					62:68	chitosan composite sponges	43:68	chitosan composite sponges	43:68	Construction of silver sulfadiazine loaded chitosan composite sponges as potential wound dressings.
27987918	2	4	theme	SEM	350:352	arg1	spectra					368:374	SEM, FTIR and XRD spectra	350:374	spectra	368:374	The co-existence of CS and AgSD in the composite sponges was confirmed by SEM, FTIR and XRD spectra, respectively.
27987918	4	5	theme	Antibacterial	574:586	arg1	property					588:595	Antibacterial property	574:595	Antibacterial property of the CS/AgSD composite sponges	574:628	Antibacterial property of the CS/AgSD composite sponges was also performed against E. coli, C. albicans, S. aureus and B. subtilis, respectively.
27987918	6	6	theme	staining	906:913	arg1	method					915:920	fluorescence staining method	893:920	fluorescence staining method	893:920	The cytotoxicity of the prepared composite sponges was performed by MTT viability assay and fluorescence staining method on HEK293 cell lines.
27987918	7	7	contain	had	1007:1009	arg2	cytotoxicity					1026:1037	no significant cytotoxicity	1011:1037	no significant cytotoxicity	1011:1037	The cell viability results indicated that the designed sponges had no significant cytotoxicity.
27987918	7	7	contain	had	1007:1009	arg1	sponges					999:1005	the designed sponges	986:1005	the designed sponges	986:1005	The cell viability results indicated that the designed sponges had no significant cytotoxicity.
27987918	6	8	theme	fluorescence	893:904	arg1	method					915:920	fluorescence staining method	893:920	fluorescence staining method	893:920	The cytotoxicity of the prepared composite sponges was performed by MTT viability assay and fluorescence staining method on HEK293 cell lines.
27987918	3	9	theme	excellent	544:552	arg1	swelling					554:561	excellent swelling	544:561	excellent swelling	544:561	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	7	10	theme	cell	948:951	arg1	results					963:969	The cell viability results	944:969	The cell viability results	944:969	The cell viability results indicated that the designed sponges had no significant cytotoxicity.
27987918	2	11	theme	CS	296:297	arg1	co-existence					280:291	The co-existence	276:291	The co-existence of CS and AgSD in the composite sponges	276:331	The co-existence of CS and AgSD in the composite sponges was confirmed by SEM, FTIR and XRD spectra, respectively.
27987918	8	12	theme	study	1060:1064	arg1	results					1044:1050	The results	1040:1050	The results of this study	1040:1064	The results of this study demonstrated that the CS/AgSD composite sponges have potential application in antimicrobial wound dressing materials.
27987918	7	13	theme	viability	953:961	arg1	results					963:969	The cell viability results	944:969	The cell viability results	944:969	The cell viability results indicated that the designed sponges had no significant cytotoxicity.
27987918	1	14	theme	AgSD	188:191	arg1	particles					193:201	AgSD particles	188:201	AgSD particles	188:201	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges were prepared by incorporating AgSD particles into CS matrix in order to develop novel biomaterials for wound healing.
27987918	5	15	theme	activity	791:798	arg1	spectrum					765:772	broad spectrum	759:772	broad spectrum of antibacterial activity	759:798	The results showed that they possessed broad spectrum of antibacterial activity.
27987918	3	16	theme	porosity	395:402	arg1	properties					417:426	The porosity and swelling properties	391:426	The porosity and swelling properties of the fabricated CS/AgSD composite sponges	391:470	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	3	17	theme	high	526:529	arg1	porosity					531:538	high porosity	526:538	high porosity	526:538	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	5	18	theme	broad	759:763	arg1	spectrum					765:772	broad spectrum	759:772	broad spectrum of antibacterial activity	759:798	The results showed that they possessed broad spectrum of antibacterial activity.
27987918	8	19	theme	wound	1158:1162	arg1	materials					1173:1181	antimicrobial wound dressing materials	1144:1181	antimicrobial wound dressing materials	1144:1181	The results of this study demonstrated that the CS/AgSD composite sponges have potential application in antimicrobial wound dressing materials.
27987918	2	20	theme	composite	315:323	arg1	sponges					325:331	the composite sponges	311:331	the composite sponges	311:331	The co-existence of CS and AgSD in the composite sponges was confirmed by SEM, FTIR and XRD spectra, respectively.
27987918	3	21	theme	swelling	408:415	arg1	properties					417:426	The porosity and swelling properties	391:426	The porosity and swelling properties of the fabricated CS/AgSD composite sponges	391:470	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	1	22	theme	Chitosan/silver	100:114	arg1	sponges					149:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges	100:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges	100:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges were prepared by incorporating AgSD particles into CS matrix in order to develop novel biomaterials for wound healing.
27987918	2	23	theme	XRD	364:366	arg1	spectra					368:374	SEM, FTIR and XRD spectra	350:374	spectra	368:374	The co-existence of CS and AgSD in the composite sponges was confirmed by SEM, FTIR and XRD spectra, respectively.
27987918	0	24	theme	sulfadiazine	23:34	arg1	Construction					0:11	Construction	0:11	Construction of silver sulfadiazine	0:34	Construction of silver sulfadiazine loaded chitosan composite sponges as potential wound dressings.
27987918	8	25	theme	CS/AgSD	1088:1094	arg1	sponges					1106:1112	the CS/AgSD composite sponges	1084:1112	the CS/AgSD composite sponges	1084:1112	The results of this study demonstrated that the CS/AgSD composite sponges have potential application in antimicrobial wound dressing materials.
27987918	1	26	theme	sulfadiazine	116:127	arg1	sponges					149:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges	100:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges	100:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges were prepared by incorporating AgSD particles into CS matrix in order to develop novel biomaterials for wound healing.
27987918	3	27	theme	composite	454:462	arg1	sponges					464:470	the fabricated CS/AgSD composite sponges	431:470	the fabricated CS/AgSD composite sponges	431:470	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	0	28	theme	silver	16:21	arg1	sulfadiazine					23:34	silver sulfadiazine	16:34	silver sulfadiazine	16:34	Construction of silver sulfadiazine loaded chitosan composite sponges as potential wound dressings.
27987918	3	29	dep	porosity	531:538	arg1	behaviors					563:571	behaviors	563:571	behaviors	563:571	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	6	30	theme	MTT	869:871	arg1	assay					883:887	MTT viability assay	869:887	MTT viability assay	869:887	The cytotoxicity of the prepared composite sponges was performed by MTT viability assay and fluorescence staining method on HEK293 cell lines.
27987918	2	31	theme	AgSD	303:306	arg1	co-existence					280:291	The co-existence	276:291	The co-existence of CS and AgSD in the composite sponges	276:331	The co-existence of CS and AgSD in the composite sponges was confirmed by SEM, FTIR and XRD spectra, respectively.
27987918	3	32	theme	sponges	464:470	arg1	properties					417:426	The porosity and swelling properties	391:426	The porosity and swelling properties of the fabricated CS/AgSD composite sponges	391:470	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	0	33	theme	chitosan	43:50	arg1	dressings					89:97	potential wound dressings	73:97	potential wound dressings	73:97	Construction of silver sulfadiazine loaded chitosan composite sponges as potential wound dressings.
27987918	0	33	theme	chitosan	43:50	arg1	sponges					62:68	chitosan composite sponges	43:68	chitosan composite sponges	43:68	Construction of silver sulfadiazine loaded chitosan composite sponges as potential wound dressings.
27987918	1	34	theme	CS	208:209	arg1	matrix					211:216	CS matrix	208:216	CS matrix in order to develop novel biomaterials for wound healing	208:273	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges were prepared by incorporating AgSD particles into CS matrix in order to develop novel biomaterials for wound healing.
27987918	3	35	theme	fabricated	435:444	arg1	sponges					464:470	the fabricated CS/AgSD composite sponges	431:470	the fabricated CS/AgSD composite sponges	431:470	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	8	36	theme	composite	1096:1104	arg1	sponges					1106:1112	the CS/AgSD composite sponges	1084:1112	the CS/AgSD composite sponges	1084:1112	The results of this study demonstrated that the CS/AgSD composite sponges have potential application in antimicrobial wound dressing materials.
27987918	8	37	theme	dressing	1164:1171	arg1	materials					1173:1181	antimicrobial wound dressing materials	1144:1181	antimicrobial wound dressing materials	1144:1181	The results of this study demonstrated that the CS/AgSD composite sponges have potential application in antimicrobial wound dressing materials.
27987918	7	38	theme	significant	1014:1024	arg1	cytotoxicity					1026:1037	no significant cytotoxicity	1011:1037	no significant cytotoxicity	1011:1037	The cell viability results indicated that the designed sponges had no significant cytotoxicity.
27987918	3	39	theme	CS/AgSD	446:452	arg1	sponges					464:470	the fabricated CS/AgSD composite sponges	431:470	the fabricated CS/AgSD composite sponges	431:470	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	5	40	theme	antibacterial	777:789	arg1	activity					791:798	antibacterial activity	777:798	antibacterial activity	777:798	The results showed that they possessed broad spectrum of antibacterial activity.
27987918	3	41	contain	had	522:524	arg2	swelling					554:561	excellent swelling	544:561	excellent swelling	544:561	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	3	41	contain	had	522:524	arg2	porosity					531:538	high porosity	526:538	high porosity	526:538	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	3	41	contain	had	522:524	arg1	they					517:520	they	517:520	they	517:520	The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors.
27987918	8	42	theme	potential	1119:1127	arg1	application					1129:1139	potential application	1119:1139	potential application	1119:1139	The results of this study demonstrated that the CS/AgSD composite sponges have potential application in antimicrobial wound dressing materials.
27987918	1	43	theme	CS/AgSD	130:136	arg1	sponges					149:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges	100:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges	100:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges were prepared by incorporating AgSD particles into CS matrix in order to develop novel biomaterials for wound healing.
27987918	0	44	theme	composite	52:60	arg1	dressings					89:97	potential wound dressings	73:97	potential wound dressings	73:97	Construction of silver sulfadiazine loaded chitosan composite sponges as potential wound dressings.
27987918	0	44	theme	composite	52:60	arg1	sponges					62:68	chitosan composite sponges	43:68	chitosan composite sponges	43:68	Construction of silver sulfadiazine loaded chitosan composite sponges as potential wound dressings.
27987918	8	45	contain	have	1114:1117	arg2	application					1129:1139	potential application	1119:1139	potential application	1119:1139	The results of this study demonstrated that the CS/AgSD composite sponges have potential application in antimicrobial wound dressing materials.
27987918	8	45	contain	have	1114:1117	arg1	sponges					1106:1112	the CS/AgSD composite sponges	1084:1112	the CS/AgSD composite sponges	1084:1112	The results of this study demonstrated that the CS/AgSD composite sponges have potential application in antimicrobial wound dressing materials.
27987918	6	46	theme	sponges	844:850	arg1	cytotoxicity					805:816	The cytotoxicity	801:816	The cytotoxicity of the prepared composite sponges	801:850	The cytotoxicity of the prepared composite sponges was performed by MTT viability assay and fluorescence staining method on HEK293 cell lines.
27987918	6	47	theme	cell	932:935	arg1	lines					937:941	HEK293 cell lines	925:941	HEK293 cell lines	925:941	The cytotoxicity of the prepared composite sponges was performed by MTT viability assay and fluorescence staining method on HEK293 cell lines.
27987918	6	48	theme	composite	834:842	arg1	sponges					844:850	the prepared composite sponges	821:850	the prepared composite sponges	821:850	The cytotoxicity of the prepared composite sponges was performed by MTT viability assay and fluorescence staining method on HEK293 cell lines.
27987918	1	49	theme	composite	139:147	arg1	sponges					149:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges	100:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges	100:155	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges were prepared by incorporating AgSD particles into CS matrix in order to develop novel biomaterials for wound healing.
27987918	2	50	from	co-existence	280:291	arg1	sponges					325:331	the composite sponges	311:331	the composite sponges	311:331	The co-existence of CS and AgSD in the composite sponges was confirmed by SEM, FTIR and XRD spectra, respectively.
27987918	4	51	theme	composite	612:620	arg1	sponges					622:628	the CS/AgSD composite sponges	600:628	the CS/AgSD composite sponges	600:628	Antibacterial property of the CS/AgSD composite sponges was also performed against E. coli, C. albicans, S. aureus and B. subtilis, respectively.
27987918	6	52	theme	prepared	825:832	arg1	sponges					844:850	the prepared composite sponges	821:850	the prepared composite sponges	821:850	The cytotoxicity of the prepared composite sponges was performed by MTT viability assay and fluorescence staining method on HEK293 cell lines.
27987918	7	53	theme	designed	990:997	arg1	sponges					999:1005	the designed sponges	986:1005	the designed sponges	986:1005	The cell viability results indicated that the designed sponges had no significant cytotoxicity.
27987918	1	54	theme	novel	238:242	arg1	biomaterials					244:255	novel biomaterials	238:255	novel biomaterials for wound healing	238:273	Chitosan/silver sulfadiazine (CS/AgSD) composite sponges were prepared by incorporating AgSD particles into CS matrix in order to develop novel biomaterials for wound healing.
27987918	0	55	theme	potential	73:81	arg1	dressings					89:97	potential wound dressings	73:97	potential wound dressings	73:97	Construction of silver sulfadiazine loaded chitosan composite sponges as potential wound dressings.
27987918	0	55	theme	potential	73:81	arg1	sponges					62:68	chitosan composite sponges	43:68	chitosan composite sponges	43:68	Construction of silver sulfadiazine loaded chitosan composite sponges as potential wound dressings.
27987918	8	56	theme	antimicrobial	1144:1156	arg1	materials					1173:1181	antimicrobial wound dressing materials	1144:1181	antimicrobial wound dressing materials	1144:1181	The results of this study demonstrated that the CS/AgSD composite sponges have potential application in antimicrobial wound dressing materials.
27987918	6	57	theme	HEK293	925:930	arg1	lines					937:941	HEK293 cell lines	925:941	HEK293 cell lines	925:941	The cytotoxicity of the prepared composite sponges was performed by MTT viability assay and fluorescence staining method on HEK293 cell lines.
27987918	2	58	theme	FTIR	355:358	arg1	spectra					368:374	SEM, FTIR and XRD spectra	350:374	spectra	368:374	The co-existence of CS and AgSD in the composite sponges was confirmed by SEM, FTIR and XRD spectra, respectively.
27987918	4	59	theme	sponges	622:628	arg1	property					588:595	Antibacterial property	574:595	Antibacterial property of the CS/AgSD composite sponges	574:628	Antibacterial property of the CS/AgSD composite sponges was also performed against E. coli, C. albicans, S. aureus and B. subtilis, respectively.
26917392	1	0	with	compatibility	220:232	arg1	materials					247:255	organic materials	239:255	organic materials	239:255	Cellulose nanocrystal (CNC) is a promising strengthener but is used limitedly since its poor compatibility with organic materials.
26917392	2	1	theme	cellulose	366:374	arg1	nanocrystal					376:386	cellulose nanocrystal	366:386	cellulose nanocrystal	366:386	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	0	2	theme	mechanism	69:77	arg1	SI-ATRP					42:48	SI-ATRP	42:48	SI-ATRP of styrene and the mechanism of its reinforcement of polymethylmethacrylate	42:124	Modification of cellulose nanocrystal via SI-ATRP of styrene and the mechanism of its reinforcement of polymethylmethacrylate.
26917392	6	3	theme	electronic	869:878	arg1	microscope					880:889	The scanning electronic microscope	856:889	The scanning electronic microscope	856:889	The scanning electronic microscope showed that the modified CNCs homogeneously dispersed in PMMA matrix.
26917392	4	4	theme	CNCs	600:603	arg1	surface					589:595	the surface	585:595	the surface of CNCs	585:603	The polymeric layer on the surface of CNCs should improve the thermal stability of CNCs, and provide significant dispersibility and compatibilization for the nanocomposites.
26917392	0	5	theme	reinforcement	86:98	arg1	mechanism					69:77	the mechanism	65:77	the mechanism of its reinforcement of polymethylmethacrylate	65:124	Modification of cellulose nanocrystal via SI-ATRP of styrene and the mechanism of its reinforcement of polymethylmethacrylate.
26917392	0	5	theme	reinforcement	86:98	arg1	styrene					53:59	styrene	53:59	styrene	53:59	Modification of cellulose nanocrystal via SI-ATRP of styrene and the mechanism of its reinforcement of polymethylmethacrylate.
26917392	6	6	theme	scanning	860:867	arg1	microscope					880:889	The scanning electronic microscope	856:889	The scanning electronic microscope	856:889	The scanning electronic microscope showed that the modified CNCs homogeneously dispersed in PMMA matrix.
26917392	5	7	theme	degrade	786:792	arg1	temperature					794:804	the initial degrade temperature	774:804	the initial degrade temperature of CNC	774:811	Thermogravimetry analysis proved that the initial degrade temperature of CNC was increased 50 °C with the modification.
26917392	7	8	theme	composites	1010:1019	arg1	strength					970:977	Breaking strength	961:977	Breaking strength	961:977	Breaking strength and elongation at break of the composites were improved, which was attributed to the reinforcement of CNCs modified with styrene.
26917392	7	8	theme	composites	1010:1019	arg1	break					997:1001	elongation at break	983:1001	elongation at break	983:1001	Breaking strength and elongation at break of the composites were improved, which was attributed to the reinforcement of CNCs modified with styrene.
26917392	2	9	theme	styrene	286:292	arg1	polymerization					268:281	The graft polymerization	258:281	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal	258:386	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	0	10	theme	polymethylmethacrylate	103:124	arg1	reinforcement					86:98	its reinforcement	82:98	its reinforcement of polymethylmethacrylate	82:124	Modification of cellulose nanocrystal via SI-ATRP of styrene and the mechanism of its reinforcement of polymethylmethacrylate.
26917392	1	11	theme	promising	160:168	arg1	nanocrystal					137:147	Cellulose nanocrystal	127:147	Cellulose nanocrystal (CNC)	127:153	Cellulose nanocrystal (CNC) is a promising strengthener but is used limitedly since its poor compatibility with organic materials.
26917392	1	11	theme	promising	160:168	arg1	strengthener					170:181	a promising strengthener	158:181	a promising strengthener	158:181	Cellulose nanocrystal (CNC) is a promising strengthener but is used limitedly since its poor compatibility with organic materials.
26917392	6	12	theme	PMMA	948:951	arg1	matrix					953:958	PMMA matrix	948:958	PMMA matrix	948:958	The scanning electronic microscope showed that the modified CNCs homogeneously dispersed in PMMA matrix.
26917392	8	13	theme	pure	1230:1233	arg1	PMMA					1235:1238	pure PMMA	1230:1238	pure PMMA	1230:1238	Transmittance of nanocomposite films measurement showed that the transmittance of PMMA/1%CNC was almost close to that of pure PMMA.
26917392	8	14	theme	films	1140:1144	arg1	measurement					1146:1156	nanocomposite films measurement	1126:1156	nanocomposite films measurement	1126:1156	Transmittance of nanocomposite films measurement showed that the transmittance of PMMA/1%CNC was almost close to that of pure PMMA.
26917392	2	15	theme	radical	330:336	arg1	SI-ATRP					354:360	SI-ATRP	354:360	SI-ATRP	354:360	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	2	15	theme	radical	330:336	arg1	polymerization					338:351	surface-initiated atom transfer radical polymerization	298:351	surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal	298:386	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	8	16	theme	nanocomposite	1126:1138	arg1	measurement					1146:1156	nanocomposite films measurement	1126:1156	nanocomposite films measurement	1126:1156	Transmittance of nanocomposite films measurement showed that the transmittance of PMMA/1%CNC was almost close to that of pure PMMA.
26917392	7	17	theme	at	994:995	arg1	break					997:1001	elongation at break	983:1001	elongation at break	983:1001	Breaking strength and elongation at break of the composites were improved, which was attributed to the reinforcement of CNCs modified with styrene.
26917392	2	18	theme	transfer	321:328	arg1	SI-ATRP					354:360	SI-ATRP	354:360	SI-ATRP	354:360	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	2	18	theme	transfer	321:328	arg1	polymerization					338:351	surface-initiated atom transfer radical polymerization	298:351	surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal	298:386	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	3	19	theme	modified	453:460	arg1	crystals					462:469	The modified crystals	449:469	The modified crystals	449:469	The modified crystals have been dosed into polymethylmethacrylate (PMMA) nanocomposites by the solution casting.
26917392	2	20	theme	atom	316:319	arg1	SI-ATRP					354:360	SI-ATRP	354:360	SI-ATRP	354:360	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	2	20	theme	atom	316:319	arg1	polymerization					338:351	surface-initiated atom transfer radical polymerization	298:351	surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal	298:386	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	0	21	theme	cellulose	16:24	arg1	Modification					0:11	Modification	0:11	Modification of cellulose	0:24	Modification of cellulose nanocrystal via SI-ATRP of styrene and the mechanism of its reinforcement of polymethylmethacrylate.
26917392	8	22	theme	measurement	1146:1156	arg1	Transmittance					1109:1121	Transmittance	1109:1121	Transmittance of nanocomposite films measurement	1109:1156	Transmittance of nanocomposite films measurement showed that the transmittance of PMMA/1%CNC was almost close to that of pure PMMA.
26917392	2	23	theme	surface-initiated	298:314	arg1	SI-ATRP					354:360	SI-ATRP	354:360	SI-ATRP	354:360	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	2	23	theme	surface-initiated	298:314	arg1	polymerization					338:351	surface-initiated atom transfer radical polymerization	298:351	surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal	298:386	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	3	24	theme	polymethylmethacrylate	492:513	arg1	nanocomposites					522:535	polymethylmethacrylate (PMMA) nanocomposites	492:535	polymethylmethacrylate (PMMA) nanocomposites	492:535	The modified crystals have been dosed into polymethylmethacrylate (PMMA) nanocomposites by the solution casting.
26917392	4	25	from	layer	576:580	arg1	surface					589:595	the surface	585:595	the surface of CNCs	585:603	The polymeric layer on the surface of CNCs should improve the thermal stability of CNCs, and provide significant dispersibility and compatibilization for the nanocomposites.
26917392	7	26	from	break	997:1001	arg1	at					994:995	elongation at break	983:1001	elongation at break	983:1001	Breaking strength and elongation at break of the composites were improved, which was attributed to the reinforcement of CNCs modified with styrene.
26917392	7	26	from	break	997:1001	arg1	elongation					983:992	elongation at break	983:1001	elongation at break	983:1001	Breaking strength and elongation at break of the composites were improved, which was attributed to the reinforcement of CNCs modified with styrene.
26917392	4	27	theme	significant	663:673	arg1	dispersibility					675:688	significant dispersibility	663:688	significant dispersibility	663:688	The polymeric layer on the surface of CNCs should improve the thermal stability of CNCs, and provide significant dispersibility and compatibilization for the nanocomposites.
26917392	0	28	theme	styrene	53:59	arg1	SI-ATRP					42:48	SI-ATRP	42:48	SI-ATRP of styrene and the mechanism of its reinforcement of polymethylmethacrylate	42:124	Modification of cellulose nanocrystal via SI-ATRP of styrene and the mechanism of its reinforcement of polymethylmethacrylate.
26917392	5	29	theme	initial	778:784	arg1	temperature					794:804	the initial degrade temperature	774:804	the initial degrade temperature of CNC	774:811	Thermogravimetry analysis proved that the initial degrade temperature of CNC was increased 50 °C with the modification.
26917392	6	30	theme	modified	907:914	arg1	CNCs					916:919	the modified CNCs	903:919	the modified CNCs	903:919	The scanning electronic microscope showed that the modified CNCs homogeneously dispersed in PMMA matrix.
26917392	7	31	theme	CNCs	1081:1084	arg1	reinforcement					1064:1076	the reinforcement	1060:1076	the reinforcement of CNCs modified with styrene	1060:1106	Breaking strength and elongation at break of the composites were improved, which was attributed to the reinforcement of CNCs modified with styrene.
26917392	1	32	used	used	190:193	arg2	CNC					150:152	CNC	150:152	CNC	150:152	Cellulose nanocrystal (CNC) is a promising strengthener but is used limitedly since its poor compatibility with organic materials.
26917392	1	32	used	used	190:193	arg2	strengthener					170:181	a promising strengthener	158:181	a promising strengthener	158:181	Cellulose nanocrystal (CNC) is a promising strengthener but is used limitedly since its poor compatibility with organic materials.
26917392	1	32	used	used	190:193	arg2	nanocrystal					137:147	Cellulose nanocrystal	127:147	Cellulose nanocrystal (CNC)	127:153	Cellulose nanocrystal (CNC) is a promising strengthener but is used limitedly since its poor compatibility with organic materials.
26917392	1	33	theme	Cellulose	127:135	arg1	nanocrystal					137:147	Cellulose nanocrystal	127:147	Cellulose nanocrystal (CNC)	127:153	Cellulose nanocrystal (CNC) is a promising strengthener but is used limitedly since its poor compatibility with organic materials.
26917392	1	33	theme	Cellulose	127:135	arg1	strengthener					170:181	a promising strengthener	158:181	a promising strengthener	158:181	Cellulose nanocrystal (CNC) is a promising strengthener but is used limitedly since its poor compatibility with organic materials.
26917392	1	33	theme	Cellulose	127:135	arg1	CNC					150:152	CNC	150:152	CNC	150:152	Cellulose nanocrystal (CNC) is a promising strengthener but is used limitedly since its poor compatibility with organic materials.
26917392	4	34	theme	polymeric	566:574	arg1	layer					576:580	The polymeric layer	562:580	The polymeric layer on the surface of CNCs	562:603	The polymeric layer on the surface of CNCs should improve the thermal stability of CNCs, and provide significant dispersibility and compatibilization for the nanocomposites.
26917392	8	35	theme	%	1197:1197	arg1	CNC					1198:1200	PMMA/1%CNC	1191:1200	PMMA/1%CNC	1191:1200	Transmittance of nanocomposite films measurement showed that the transmittance of PMMA/1%CNC was almost close to that of pure PMMA.
26917392	2	36	theme	graft	262:266	arg1	polymerization					268:281	The graft polymerization	258:281	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal	258:386	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	8	37	theme	PMMA/1	1191:1196	arg1	%					1197:1197	%	1197:1197	%	1197:1197	Transmittance of nanocomposite films measurement showed that the transmittance of PMMA/1%CNC was almost close to that of pure PMMA.
26917392	7	38	theme	elongation	983:992	arg1	break					997:1001	elongation at break	983:1001	elongation at break	983:1001	Breaking strength and elongation at break of the composites were improved, which was attributed to the reinforcement of CNCs modified with styrene.
26917392	1	39	theme	poor	215:218	arg1	compatibility					220:232	its poor compatibility	211:232	its poor compatibility with organic materials	211:255	Cellulose nanocrystal (CNC) is a promising strengthener but is used limitedly since its poor compatibility with organic materials.
26917392	5	40	theme	Thermogravimetry	736:751	arg1	analysis					753:760	Thermogravimetry analysis	736:760	Thermogravimetry analysis	736:760	Thermogravimetry analysis proved that the initial degrade temperature of CNC was increased 50 °C with the modification.
26917392	7	41	theme	Breaking	961:968	arg1	strength					970:977	Breaking strength	961:977	Breaking strength	961:977	Breaking strength and elongation at break of the composites were improved, which was attributed to the reinforcement of CNCs modified with styrene.
26917392	4	42	theme	thermal	624:630	arg1	stability					632:640	the thermal stability	620:640	the thermal stability of CNCs	620:648	The polymeric layer on the surface of CNCs should improve the thermal stability of CNCs, and provide significant dispersibility and compatibilization for the nanocomposites.
26917392	5	43	theme	CNC	809:811	arg1	temperature					794:804	the initial degrade temperature	774:804	the initial degrade temperature of CNC	774:811	Thermogravimetry analysis proved that the initial degrade temperature of CNC was increased 50 °C with the modification.
26917392	3	44	theme	solution	544:551	arg1	casting					553:559	the solution casting	540:559	the solution casting	540:559	The modified crystals have been dosed into polymethylmethacrylate (PMMA) nanocomposites by the solution casting.
26917392	4	45	theme	CNCs	645:648	arg1	stability					632:640	the thermal stability	620:640	the thermal stability of CNCs	620:648	The polymeric layer on the surface of CNCs should improve the thermal stability of CNCs, and provide significant dispersibility and compatibilization for the nanocomposites.
26917392	8	46	theme	CNC	1198:1200	arg1	that					1222:1225	that	1222:1225	that	1222:1225	Transmittance of nanocomposite films measurement showed that the transmittance of PMMA/1%CNC was almost close to that of pure PMMA.
26917392	8	46	theme	CNC	1198:1200	arg1	transmittance					1174:1186	the transmittance	1170:1186	the transmittance of PMMA/1%CNC	1170:1200	Transmittance of nanocomposite films measurement showed that the transmittance of PMMA/1%CNC was almost close to that of pure PMMA.
26917392	2	47	theme	nanocrystal	376:386	arg1	SI-ATRP					354:360	SI-ATRP	354:360	SI-ATRP	354:360	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	2	47	theme	nanocrystal	376:386	arg1	polymerization					338:351	surface-initiated atom transfer radical polymerization	298:351	surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal	298:386	The graft polymerization of styrene via surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose nanocrystal is adopted to modify its thermo-stability and compatibility.
26917392	1	48	theme	organic	239:245	arg1	materials					247:255	organic materials	239:255	organic materials	239:255	Cellulose nanocrystal (CNC) is a promising strengthener but is used limitedly since its poor compatibility with organic materials.
26478418	4	0	theme	scanning	516:523	arg1	microscopy					534:543	scanning electron microscopy	516:543	scanning electron microscopy	516:543	The microstructure of composite scaffold was characterized using scanning electron microscopy.
26478418	1	1	theme	chitosan	167:174	arg1	sponge					176:181	chitosan sponge	167:181	chitosan sponge	167:181	A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge was fabricated using a combination of electron beam melting and freeze-drying.
26478418	8	2	theme	osteoblasts	870:880	arg1	superior					909:916	superior	909:916	superior	909:916	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	8	2	theme	osteoblasts	870:880	arg1	response					858:865	The biological response	843:865	The biological response of osteoblasts on composite scaffolds	843:903	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	5	3	theme	micro-pore	637:646	arg1	architecture					648:659	highly porous and well-interconnected micro-pore architecture	599:659	highly porous and well-interconnected micro-pore architecture	599:659	The chitosan sponge filled in Ti6Al4V part exhibited highly porous and well-interconnected micro-pore architecture.
26478418	8	4	theme	cell	939:942	arg1	attachment					944:953	improved cell attachment	930:953	improved cell attachment	930:953	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	3	5	theme	85.35	385:389	arg1	±					391:391	±	391:391	±	391:391	The ultimate compressive strength was 85.35 ± 8.68 MPa and the compressive modulus was 2.26 ± 0.42 GPa.
26478418	6	6	theme	osteoblastic	666:677	arg1	cells					679:683	The osteoblastic cells	662:683	The osteoblastic cells	662:683	The osteoblastic cells were seeded on scaffolds to test their seeding efficiency and biocompatibility.
26478418	7	7	theme	cell	786:789	arg1	efficiency					799:808	Significantly higher cell seeding efficiency	765:808	Significantly higher cell seeding efficiency	765:808	Significantly higher cell seeding efficiency was found on composite scaffold.
26478418	8	8	from	superior	909:916	arg1	terms					921:925	terms	921:925	terms of improved cell attachment, higher proliferation, and well-spread morphology	921:1003	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	2	9	theme	part	306:309	arg1	properties					277:286	The mechanical properties	262:286	The mechanical properties of porous Ti6Al4V part	262:309	The mechanical properties of porous Ti6Al4V part were examined via compressive test.
26478418	7	10	theme	seeding	791:797	arg1	efficiency					799:808	Significantly higher cell seeding efficiency	765:808	Significantly higher cell seeding efficiency	765:808	Significantly higher cell seeding efficiency was found on composite scaffold.
26478418	4	11	theme	scaffold	483:490	arg1	microstructure					455:468	The microstructure	451:468	The microstructure of composite scaffold	451:490	The microstructure of composite scaffold was characterized using scanning electron microscopy.
26478418	2	12	theme	Ti6Al4V	298:304	arg1	part					306:309	porous Ti6Al4V part	291:309	porous Ti6Al4V part	291:309	The mechanical properties of porous Ti6Al4V part were examined via compressive test.
26478418	8	13	theme	attachment	944:953	arg1	terms					921:925	terms	921:925	terms of improved cell attachment, higher proliferation, and well-spread morphology	921:1003	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	9	14	theme	Ti6Al4V/chitosan	1072:1087	arg1	scaffold					1099:1106	the Ti6Al4V/chitosan composite scaffold	1068:1106	the Ti6Al4V/chitosan composite scaffold	1068:1106	These results suggest that the Ti6Al4V/chitosan composite scaffold is potentially useful as a biomedical scaffold for orthopedic applications.
26478418	9	14	theme	Ti6Al4V/chitosan	1072:1087	arg1	useful					1123:1128	useful	1123:1128	useful	1123:1128	These results suggest that the Ti6Al4V/chitosan composite scaffold is potentially useful as a biomedical scaffold for orthopedic applications.
26478418	2	15	theme	porous	291:296	arg1	part					306:309	porous Ti6Al4V part	291:309	porous Ti6Al4V part	291:309	The mechanical properties of porous Ti6Al4V part were examined via compressive test.
26478418	8	16	theme	proliferation	963:975	arg1	terms					921:925	terms	921:925	terms of improved cell attachment, higher proliferation, and well-spread morphology	921:1003	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	5	17	theme	porous	606:611	arg1	architecture					648:659	highly porous and well-interconnected micro-pore architecture	599:659	highly porous and well-interconnected micro-pore architecture	599:659	The chitosan sponge filled in Ti6Al4V part exhibited highly porous and well-interconnected micro-pore architecture.
26478418	1	18	theme	novel	96:100	arg1	scaffold					112:119	A novel composite scaffold	94:119	A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge	94:181	A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge was fabricated using a combination of electron beam melting and freeze-drying.
26478418	8	19	theme	higher	956:961	arg1	proliferation					963:975	higher proliferation	956:975	higher proliferation	956:975	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	3	20	theme	±	439:439	arg1	GPa					446:448	2.26 ± 0.42 GPa	434:448	2.26 ± 0.42 GPa	434:448	The ultimate compressive strength was 85.35 ± 8.68 MPa and the compressive modulus was 2.26 ± 0.42 GPa.
26478418	3	20	theme	±	439:439	arg1	modulus					422:428	the compressive modulus	406:428	the compressive modulus	406:428	The ultimate compressive strength was 85.35 ± 8.68 MPa and the compressive modulus was 2.26 ± 0.42 GPa.
26478418	2	21	theme	compressive	329:339	arg1	test					341:344	compressive test	329:344	compressive test	329:344	The mechanical properties of porous Ti6Al4V part were examined via compressive test.
26478418	3	22	theme	8.68	393:396	arg1	±					391:391	±	391:391	±	391:391	The ultimate compressive strength was 85.35 ± 8.68 MPa and the compressive modulus was 2.26 ± 0.42 GPa.
26478418	8	23	theme	morphology	994:1003	arg1	terms					921:925	terms	921:925	terms of improved cell attachment, higher proliferation, and well-spread morphology	921:1003	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	5	24	theme	well-interconnected	617:635	arg1	architecture					648:659	highly porous and well-interconnected micro-pore architecture	599:659	highly porous and well-interconnected micro-pore architecture	599:659	The chitosan sponge filled in Ti6Al4V part exhibited highly porous and well-interconnected micro-pore architecture.
26478418	1	25	theme	composite	102:110	arg1	scaffold					112:119	A novel composite scaffold	94:119	A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge	94:181	A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge was fabricated using a combination of electron beam melting and freeze-drying.
26478418	8	26	from	terms	921:925	arg1	superior					909:916	superior	909:916	superior	909:916	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	8	26	from	terms	921:925	arg1	response					858:865	The biological response	843:865	The biological response of osteoblasts on composite scaffolds	843:903	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	8	27	theme	well-spread	982:992	arg1	morphology					994:1003	well-spread morphology	982:1003	well-spread morphology	982:1003	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	6	28	theme	seeding	724:730	arg1	efficiency					732:741	their seeding efficiency	718:741	their seeding efficiency	718:741	The osteoblastic cells were seeded on scaffolds to test their seeding efficiency and biocompatibility.
26478418	0	29	theme	composite	46:54	arg1	scaffold					56:63	composite scaffold	46:63	composite scaffold for orthopedic applications	46:91	Development of porous Ti6Al4V/chitosan sponge composite scaffold for orthopedic applications.
26478418	7	30	theme	higher	779:784	arg1	efficiency					799:808	Significantly higher cell seeding efficiency	765:808	Significantly higher cell seeding efficiency	765:808	Significantly higher cell seeding efficiency was found on composite scaffold.
26478418	8	31	theme	Ti6Al4V	1027:1033	arg1	part					1035:1038	porous Ti6Al4V part	1020:1038	porous Ti6Al4V part	1020:1038	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	1	32	theme	electron	221:228	arg1	melting					235:241	electron beam melting	221:241	electron beam melting	221:241	A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge was fabricated using a combination of electron beam melting and freeze-drying.
26478418	9	33	theme	composite	1089:1097	arg1	scaffold					1099:1106	the Ti6Al4V/chitosan composite scaffold	1068:1106	the Ti6Al4V/chitosan composite scaffold	1068:1106	These results suggest that the Ti6Al4V/chitosan composite scaffold is potentially useful as a biomedical scaffold for orthopedic applications.
26478418	9	33	theme	composite	1089:1097	arg1	useful					1123:1128	useful	1123:1128	useful	1123:1128	These results suggest that the Ti6Al4V/chitosan composite scaffold is potentially useful as a biomedical scaffold for orthopedic applications.
26478418	4	34	theme	composite	473:481	arg1	scaffold					483:490	composite scaffold	473:490	composite scaffold	473:490	The microstructure of composite scaffold was characterized using scanning electron microscopy.
26478418	0	35	theme	Ti6Al4V/chitosan	22:37	arg1	Development					0:10	Development	0:10	Development of porous Ti6Al4V/chitosan	0:37	Development of porous Ti6Al4V/chitosan sponge composite scaffold for orthopedic applications.
26478418	1	36	theme	beam	230:233	arg1	melting					235:241	electron beam melting	221:241	electron beam melting	221:241	A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge was fabricated using a combination of electron beam melting and freeze-drying.
26478418	3	37	theme	compressive	410:420	arg1	GPa					446:448	2.26 ± 0.42 GPa	434:448	2.26 ± 0.42 GPa	434:448	The ultimate compressive strength was 85.35 ± 8.68 MPa and the compressive modulus was 2.26 ± 0.42 GPa.
26478418	3	37	theme	compressive	410:420	arg1	modulus					422:428	the compressive modulus	406:428	the compressive modulus	406:428	The ultimate compressive strength was 85.35 ± 8.68 MPa and the compressive modulus was 2.26 ± 0.42 GPa.
26478418	3	38	theme	compressive	360:370	arg1	MPa					398:400	85.35 ± 8.68 MPa	385:400	85.35 ± 8.68 MPa	385:400	The ultimate compressive strength was 85.35 ± 8.68 MPa and the compressive modulus was 2.26 ± 0.42 GPa.
26478418	3	38	theme	compressive	360:370	arg1	strength					372:379	The ultimate compressive strength	347:379	The ultimate compressive strength	347:379	The ultimate compressive strength was 85.35 ± 8.68 MPa and the compressive modulus was 2.26 ± 0.42 GPa.
26478418	5	39	theme	Ti6Al4V	576:582	arg1	part					584:587	Ti6Al4V part	576:587	Ti6Al4V part	576:587	The chitosan sponge filled in Ti6Al4V part exhibited highly porous and well-interconnected micro-pore architecture.
26478418	8	40	from	response	858:865	arg1	scaffolds					895:903	composite scaffolds	885:903	composite scaffolds	885:903	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	8	41	theme	porous	1020:1025	arg1	part					1035:1038	porous Ti6Al4V part	1020:1038	porous Ti6Al4V part	1020:1038	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	1	42	theme	porous	135:140	arg1	part					150:153	porous Ti6Al4V part	135:153	porous Ti6Al4V part filled with chitosan sponge	135:181	A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge was fabricated using a combination of electron beam melting and freeze-drying.
26478418	8	43	theme	composite	885:893	arg1	scaffolds					895:903	composite scaffolds	885:903	composite scaffolds	885:903	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	7	44	located	found	814:818	arg2	efficiency					799:808	Significantly higher cell seeding efficiency	765:808	Significantly higher cell seeding efficiency	765:808	Significantly higher cell seeding efficiency was found on composite scaffold.
26478418	7	44	located	found	814:818	arg1	scaffold					833:840	composite scaffold	823:840	composite scaffold	823:840	Significantly higher cell seeding efficiency was found on composite scaffold.
26478418	9	45	theme	orthopedic	1159:1168	arg1	applications					1170:1181	orthopedic applications	1159:1181	orthopedic applications	1159:1181	These results suggest that the Ti6Al4V/chitosan composite scaffold is potentially useful as a biomedical scaffold for orthopedic applications.
26478418	1	46	theme	Ti6Al4V	142:148	arg1	part					150:153	porous Ti6Al4V part	135:153	porous Ti6Al4V part filled with chitosan sponge	135:181	A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge was fabricated using a combination of electron beam melting and freeze-drying.
26478418	1	47	theme	melting	235:241	arg1	freeze-drying					247:259	freeze-drying	247:259	freeze-drying	247:259	A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge was fabricated using a combination of electron beam melting and freeze-drying.
26478418	1	47	theme	melting	235:241	arg1	combination					206:216	a combination	204:216	a combination of electron beam melting	204:241	A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge was fabricated using a combination of electron beam melting and freeze-drying.
26478418	8	48	dep	part	1035:1038	arg1	relation					1008:1015	relation	1008:1015	relation	1008:1015	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	3	49	theme	ultimate	351:358	arg1	MPa					398:400	85.35 ± 8.68 MPa	385:400	85.35 ± 8.68 MPa	385:400	The ultimate compressive strength was 85.35 ± 8.68 MPa and the compressive modulus was 2.26 ± 0.42 GPa.
26478418	3	49	theme	ultimate	351:358	arg1	strength					372:379	The ultimate compressive strength	347:379	The ultimate compressive strength	347:379	The ultimate compressive strength was 85.35 ± 8.68 MPa and the compressive modulus was 2.26 ± 0.42 GPa.
26478418	8	50	theme	improved	930:937	arg1	attachment					944:953	improved cell attachment	930:953	improved cell attachment	930:953	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	2	51	theme	mechanical	266:275	arg1	properties					277:286	The mechanical properties	262:286	The mechanical properties of porous Ti6Al4V part	262:309	The mechanical properties of porous Ti6Al4V part were examined via compressive test.
26478418	5	52	theme	chitosan	550:557	arg1	sponge					559:564	The chitosan sponge	546:564	The chitosan sponge filled in Ti6Al4V part	546:587	The chitosan sponge filled in Ti6Al4V part exhibited highly porous and well-interconnected micro-pore architecture.
26478418	0	53	theme	orthopedic	69:78	arg1	applications					80:91	orthopedic applications	69:91	orthopedic applications	69:91	Development of porous Ti6Al4V/chitosan sponge composite scaffold for orthopedic applications.
26478418	4	54	theme	electron	525:532	arg1	microscopy					534:543	scanning electron microscopy	516:543	scanning electron microscopy	516:543	The microstructure of composite scaffold was characterized using scanning electron microscopy.
26478418	0	55	theme	porous	15:20	arg1	Ti6Al4V/chitosan					22:37	porous Ti6Al4V/chitosan	15:37	porous Ti6Al4V/chitosan	15:37	Development of porous Ti6Al4V/chitosan sponge composite scaffold for orthopedic applications.
26478418	7	56	theme	composite	823:831	arg1	scaffold					833:840	composite scaffold	823:840	composite scaffold	823:840	Significantly higher cell seeding efficiency was found on composite scaffold.
26478418	9	57	theme	biomedical	1135:1144	arg1	scaffold					1146:1153	a biomedical scaffold	1133:1153	a biomedical scaffold for orthopedic applications	1133:1181	These results suggest that the Ti6Al4V/chitosan composite scaffold is potentially useful as a biomedical scaffold for orthopedic applications.
26478418	8	58	theme	biological	847:856	arg1	superior					909:916	superior	909:916	superior	909:916	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
26478418	8	58	theme	biological	847:856	arg1	response					858:865	The biological response	843:865	The biological response of osteoblasts on composite scaffolds	843:903	The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part.
24710601	2	0	theme	starch	539:544	arg1	starch					566:571	250 g kg(-1) starch	553:571	250 g kg(-1) starch	553:571	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	2	0	theme	starch	539:544	arg1	level					546:550	an equal starch level	530:550	an equal starch level (250 g kg(-1) starch)	530:572	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	9	1	theme	aspartate	1678:1686	arg1	aminotransferase					1688:1703	aspartate aminotransferase	1678:1703	aspartate aminotransferase	1678:1703	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	2	2	with	diets	513:517	arg1	diets					601:605	different AM/AP ratio diets	579:605	different AM/AP ratio diets	579:605	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	13	3	theme	puffer	2483:2488	arg1	growth					2465:2470	maximum growth	2457:2470	maximum growth of obscure puffer	2457:2488	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	9	4	theme	total	1528:1532	arg1	concentration					1545:1557	higher plasma total amino acid concentration	1514:1557	higher plasma total amino acid concentration than fish	1514:1567	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	2	5	theme	g	557:557	arg1	level					546:550	an equal starch level	530:550	an equal starch level (250 g kg(-1) starch)	530:572	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	2	5	theme	g	557:557	arg1	starch					566:571	250 g kg(-1) starch	553:571	250 g kg(-1) starch	553:571	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	6	6	theme	liver	994:998	arg1	glycogen					1000:1007	liver glycogen and lipid content	994:1025	glycogen	1000:1007	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	2	7	theme	9/16	628:631	arg1	diets					513:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets	423:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13	423:641	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	7	8	theme	intestinal	1220:1229	arg1	trypsin					1231:1237	intestinal trypsin	1220:1237	intestinal trypsin	1220:1237	Activities of liver and intestinal trypsin in fish fed diet 3/22 and diet 6/19 were higher than in fish fed diet 9/16 and diet 12/13.
24710601	2	9	theme	6/19	622:625	arg1	diets					513:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets	423:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13	423:641	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	14	10	theme	obscure	2716:2722	arg1	puffer					2724:2729	juvenile obscure puffer	2707:2729	juvenile obscure puffer	2707:2729	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	1	11	theme	Takifugu	404:411	arg1	puffer					396:401	juvenile obscure puffer	379:401	juvenile obscure puffer	379:401	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	1	11	theme	Takifugu	404:411	arg1	obscurus					413:420	Takifugu obscurus	404:420	Takifugu obscurus	404:420	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	13	12	theme	maximum	2457:2463	arg1	growth					2465:2470	maximum growth	2457:2470	maximum growth of obscure puffer	2457:2488	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	7	13	theme	liver	1210:1214	arg1	Activities					1196:1205	Activities	1196:1205	Activities of liver and intestinal trypsin in fish fed diet 3/22 and diet 6/19	1196:1273	Activities of liver and intestinal trypsin in fish fed diet 3/22 and diet 6/19 were higher than in fish fed diet 9/16 and diet 12/13.
24710601	10	14	theme	viscerosomatic	1760:1773	arg1	index					1775:1779	viscerosomatic index	1760:1779	viscerosomatic index	1760:1779	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	0	15	theme	postprandial	109:120	arg1	responses					132:140	postprandial metabolic responses	109:140	postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus	109:185	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	5	16	theme	growth	879:884	arg1	ratio					926:930	best growth, feed efficiency and protein efficiency ratio	874:930	best growth, feed efficiency and protein efficiency ratio	874:930	Fish fed diet 6/19 showed best growth, feed efficiency and protein efficiency ratio.
24710601	14	17	from	activities	2693:2702	arg1	puffer					2724:2729	juvenile obscure puffer	2707:2729	juvenile obscure puffer	2707:2729	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	1	18	theme	dietary	202:208	arg1	ratio					238:242	dietary amylose/amylopectin (AM/AP) ratio	202:242	dietary amylose/amylopectin (AM/AP) ratio	202:242	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	0	19	from	utilization	73:83	arg1	obscurus					178:185	juvenile obscure puffer Takifugu obscurus	145:185	juvenile obscure puffer Takifugu obscurus	145:185	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	6	20	theme	kinase	1056:1061	arg1	activities					1095:1104	gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities	1032:1104	gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities	1032:1104	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	2	21	theme	12/13	637:641	arg1	diets					513:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets	423:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13	423:641	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	10	22	theme	whole	1803:1807	arg1	body					1809:1812	whole body and muscle composition	1803:1835	body	1809:1812	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	14	23	theme	enzyme	2686:2691	arg1	activities					2693:2702	hepatic glucose metabolic enzyme activities	2660:2702	utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer	2595:2729	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	5	24	theme	feed	887:890	arg1	efficiency					892:901	feed efficiency	887:901	feed efficiency	887:901	Fish fed diet 6/19 showed best growth, feed efficiency and protein efficiency ratio.
24710601	13	25	theme	dietary	2400:2406	arg1	ratio					2414:2418	dietary AM/AP ratio	2400:2418	dietary AM/AP ratio	2400:2418	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	0	26	theme	Takifugu	169:176	arg1	obscurus					178:185	juvenile obscure puffer Takifugu obscurus	145:185	juvenile obscure puffer Takifugu obscurus	145:185	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	0	27	theme	juvenile	145:152	arg1	obscurus					178:185	juvenile obscure puffer Takifugu obscurus	145:185	juvenile obscure puffer Takifugu obscurus	145:185	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	5	28	theme	diet	857:860	arg1	6/19					862:865	Fish fed diet 6/19	848:865	Fish fed diet 6/19	848:865	Fish fed diet 6/19 showed best growth, feed efficiency and protein efficiency ratio.
24710601	3	29	theme	fish	716:719	arg1	tank					725:728	25 fish per tank	713:728	25 fish per tank	713:728	Each experimental diet was fed to triplicate groups (25 fish per tank), twice daily during a period of 60 days.
24710601	3	29	theme	fish	716:719	arg1	groups					705:710	triplicate groups	694:710	triplicate groups (25 fish per tank)	694:729	Each experimental diet was fed to triplicate groups (25 fish per tank), twice daily during a period of 60 days.
24710601	9	30	theme	plasma	1521:1526	arg1	concentration					1545:1557	higher plasma total amino acid concentration	1514:1557	higher plasma total amino acid concentration than fish	1514:1567	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	10	31	theme	hexokinase	1943:1952	arg1	composition					1825:1835	whole body and muscle composition	1803:1835	composition	1825:1835	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	31	theme	hexokinase	1943:1952	arg1	factor					1795:1800	condition factor	1785:1800	condition factor	1785:1800	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	31	theme	hexokinase	1943:1952	arg1	concentrations					1898:1911	plasma high-density and low-density lipoprotein cholesterol concentrations	1838:1911	plasma high-density and low-density lipoprotein cholesterol concentrations	1838:1911	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	31	theme	hexokinase	1943:1952	arg1	index					1775:1779	viscerosomatic index	1760:1779	viscerosomatic index	1760:1779	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	31	theme	hexokinase	1943:1952	arg1	body					1809:1812	whole body and muscle composition	1803:1835	body	1809:1812	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	31	theme	hexokinase	1943:1952	arg1	activities					1918:1927	activities	1918:1927	activities	1918:1927	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	31	theme	hexokinase	1943:1952	arg1	glucose-6-phosphatase					1958:1978	glucose-6-phosphatase	1958:1978	glucose-6-phosphatase in liver	1958:1987	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	2	32	theme	AM/AP	589:593	arg1	diets					601:605	different AM/AP ratio diets	579:605	different AM/AP ratio diets	579:605	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	6	33	theme	highest	1129:1135	arg1	diet					1143:1146	highest AM/AP diet	1129:1146	highest AM/AP diet (12/13)	1129:1154	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	6	33	theme	highest	1129:1135	arg1	12/13					1149:1153	12/13	1149:1153	12/13	1149:1153	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	7	34	dep	diet	1251:1254	arg1	6/19					1270:1273	6/19	1270:1273	6/19	1270:1273	Activities of liver and intestinal trypsin in fish fed diet 3/22 and diet 6/19 were higher than in fish fed diet 9/16 and diet 12/13.
24710601	9	35	theme	amino	1534:1538	arg1	concentration					1545:1557	higher plasma total amino acid concentration	1514:1557	higher plasma total amino acid concentration than fish	1514:1567	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	14	36	theme	juvenile	2707:2714	arg1	puffer					2724:2729	juvenile obscure puffer	2707:2729	juvenile obscure puffer	2707:2729	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	2	37	theme	3/22	616:619	arg1	diets					513:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets	423:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13	423:641	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	3	38	theme	per	721:723	arg1	tank					725:728	25 fish per tank	713:728	25 fish per tank	713:728	Each experimental diet was fed to triplicate groups (25 fish per tank), twice daily during a period of 60 days.
24710601	3	38	theme	per	721:723	arg1	groups					705:710	triplicate groups	694:710	triplicate groups (25 fish per tank)	694:729	Each experimental diet was fed to triplicate groups (25 fish per tank), twice daily during a period of 60 days.
24710601	13	39	theme	AM/AP	2441:2445	arg1	0.25					2494:2497	0.25	2494:2497	0.25	2494:2497	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	13	39	theme	AM/AP	2441:2445	arg1	ratio					2447:2451	the optimum dietary AM/AP ratio	2421:2451	the optimum dietary AM/AP ratio for maximum growth of obscure puffer	2421:2488	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	9	40	theme	plasma	1596:1601	arg1	concentration					1617:1629	plasma urea nitrogen concentration	1596:1629	plasma urea nitrogen concentration	1596:1629	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	13	41	theme	AM/AP	2408:2412	arg1	ratio					2414:2418	dietary AM/AP ratio	2400:2418	dietary AM/AP ratio	2400:2418	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	4	42	theme	blood	811:815	arg1	test					826:829	a postprandial blood response test	796:829	a postprandial blood response test	796:829	After the growth trial, a postprandial blood response test was carried out.
24710601	2	43	theme	0/25	610:613	arg1	diets					513:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets	423:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13	423:641	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	3	44	theme	triplicate	694:703	arg1	tank					725:728	25 fish per tank	713:728	25 fish per tank	713:728	Each experimental diet was fed to triplicate groups (25 fish per tank), twice daily during a period of 60 days.
24710601	3	44	theme	triplicate	694:703	arg1	groups					705:710	triplicate groups	694:710	triplicate groups (25 fish per tank)	694:729	Each experimental diet was fed to triplicate groups (25 fish per tank), twice daily during a period of 60 days.
24710601	10	45	from	body	1809:1812	arg1	liver					1983:1987	liver	1983:1987	liver	1983:1987	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	1	46	theme	blood	338:342	arg1	responses					352:360	postprandial blood glucose responses	325:360	postprandial blood glucose responses	325:360	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	2	47	theme	g	491:491	arg1	lipid					506:510	90 g kg(-1) crude lipid	488:510	90 g kg(-1) crude lipid	488:510	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	0	48	from	Effects	0:6	arg1	responses					132:140	postprandial metabolic responses	109:140	postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus	109:185	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	0	48	from	Effects	0:6	arg1	utilization					73:83	feed utilization	68:83	feed utilization	68:83	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	0	48	from	Effects	0:6	arg1	enzymes					96:102	digestive enzymes	86:102	digestive enzymes	86:102	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	0	48	from	Effects	0:6	arg1	performance					55:65	growth performance	48:65	growth performance	48:65	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	10	49	theme	plasma	1838:1843	arg1	concentrations					1898:1911	plasma high-density and low-density lipoprotein cholesterol concentrations	1838:1911	plasma high-density and low-density lipoprotein cholesterol concentrations	1838:1911	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	0	50	theme	digestive	86:94	arg1	enzymes					96:102	digestive enzymes	86:102	digestive enzymes	86:102	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	10	51	from	index	1775:1779	arg1	liver					1983:1987	liver	1983:1987	liver	1983:1987	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	52	from	concentrations	1898:1911	arg1	liver					1983:1987	liver	1983:1987	liver	1983:1987	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	13	53	theme	optimum	2425:2431	arg1	0.25					2494:2497	0.25	2494:2497	0.25	2494:2497	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	13	53	theme	optimum	2425:2431	arg1	ratio					2447:2451	the optimum dietary AM/AP ratio	2421:2451	the optimum dietary AM/AP ratio for maximum growth of obscure puffer	2421:2488	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	5	54	theme	efficiency	915:924	arg1	ratio					926:930	best growth, feed efficiency and protein efficiency ratio	874:930	best growth, feed efficiency and protein efficiency ratio	874:930	Fish fed diet 6/19 showed best growth, feed efficiency and protein efficiency ratio.
24710601	2	55	dep	isonitrogenous	428:441	arg1	protein					463:469	430 g kg(-1) crude protein	444:469	430 g kg(-1) crude protein	444:469	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	14	56	theme	AM/AP	2542:2546	arg1	ratio					2548:2552	dietary AM/AP ratio	2534:2552	dietary AM/AP ratio	2534:2552	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	2	57	theme	g	448:448	arg1	protein					463:469	430 g kg(-1) crude protein	444:469	430 g kg(-1) crude protein	444:469	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	12	58	theme	significant	2256:2266	arg1	difference					2268:2277	a significant difference	2254:2277	a significant difference	2254:2277	Plasma glucose and triglyceride concentrations showed a significant difference at 2 and 4 h after a meal and varied between dietary treatments.
24710601	13	59	theme	dietary	2433:2439	arg1	0.25					2494:2497	0.25	2494:2497	0.25	2494:2497	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	13	59	theme	dietary	2433:2439	arg1	ratio					2447:2451	the optimum dietary AM/AP ratio	2421:2451	the optimum dietary AM/AP ratio for maximum growth of obscure puffer	2421:2488	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	14	60	theme	metabolic	2676:2684	arg1	activities					2693:2702	hepatic glucose metabolic enzyme activities	2660:2702	utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer	2595:2729	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	3	61	theme	days	766:769	arg1	period					753:758	a period	751:758	a period of 60 days	751:769	Each experimental diet was fed to triplicate groups (25 fish per tank), twice daily during a period of 60 days.
24710601	11	62	theme	Postprandial	1990:2001	arg1	glucose					2010:2016	Postprandial plasma glucose	1990:2016	Postprandial plasma glucose	1990:2016	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	0	63	theme	dietary	11:17	arg1	ratio					39:43	dietary amylose/amylopectin ratio	11:43	dietary amylose/amylopectin ratio	11:43	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	1	64	theme	enzyme	283:288	arg1	activities					290:299	digestive enzyme activities	273:299	digestive enzyme activities	273:299	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	2	65	dep	isolipidic	476:485	arg1	lipid					506:510	90 g kg(-1) crude lipid	488:510	90 g kg(-1) crude lipid	488:510	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	7	66	from	Activities	1196:1205	arg1	fish					1242:1245	fish	1242:1245	fish fed diet 3/22 and diet 6/19	1242:1273	Activities of liver and intestinal trypsin in fish fed diet 3/22 and diet 6/19 were higher than in fish fed diet 9/16 and diet 12/13.
24710601	9	67	theme	urea	1603:1606	arg1	concentration					1617:1629	plasma urea nitrogen concentration	1596:1629	plasma urea nitrogen concentration	1596:1629	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	8	68	theme	liver	1344:1348	arg1	Activities					1330:1339	Activities	1330:1339	Activities of liver and intestinal amylase and intestinal lipase	1330:1393	Activities of liver and intestinal amylase and intestinal lipase, and starch digestibility were negatively correlated with dietary AM/AP ratio.
24710601	8	68	theme	liver	1344:1348	arg1	digestibility					1407:1419	starch digestibility	1400:1419	starch digestibility	1400:1419	Activities of liver and intestinal amylase and intestinal lipase, and starch digestibility were negatively correlated with dietary AM/AP ratio.
24710601	0	69	theme	ratio	39:43	arg1	Effects					0:6	Effects	0:6	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.	0:186	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	9	70	theme	nitrogen	1608:1615	arg1	concentration					1617:1629	plasma urea nitrogen concentration	1596:1629	plasma urea nitrogen concentration	1596:1629	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	11	71	theme	triglyceride	2022:2033	arg1	value					2040:2044	Postprandial plasma glucose and triglyceride peak value	1990:2044	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13	1990:2067	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	8	72	theme	intestinal	1354:1363	arg1	amylase					1365:1371	intestinal amylase	1354:1371	intestinal amylase	1354:1371	Activities of liver and intestinal amylase and intestinal lipase, and starch digestibility were negatively correlated with dietary AM/AP ratio.
24710601	0	73	theme	growth	48:53	arg1	performance					55:65	growth performance	48:65	growth performance	48:65	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	5	74	theme	fed	853:855	arg1	6/19					862:865	Fish fed diet 6/19	848:865	Fish fed diet 6/19	848:865	Fish fed diet 6/19 showed best growth, feed efficiency and protein efficiency ratio.
24710601	12	75	theme	triglyceride	2219:2230	arg1	concentrations					2232:2245	Plasma glucose and triglyceride concentrations	2200:2245	concentrations	2232:2245	Plasma glucose and triglyceride concentrations showed a significant difference at 2 and 4 h after a meal and varied between dietary treatments.
24710601	11	76	theme	plasma	2003:2008	arg1	glucose					2010:2016	Postprandial plasma glucose	1990:2016	Postprandial plasma glucose	1990:2016	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	14	77	from	utilization	2595:2605	arg1	puffer					2724:2729	juvenile obscure puffer	2707:2729	juvenile obscure puffer	2707:2729	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	14	78	theme	plasma	2613:2618	arg1	parameters					2620:2629	some plasma parameters	2608:2629	some plasma parameters	2608:2629	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	14	78	theme	plasma	2613:2618	arg1	utilization					2595:2605	utilization	2595:2605	utilization	2595:2605	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	1	79	theme	AM/AP	231:235	arg1	ratio					238:242	dietary amylose/amylopectin (AM/AP) ratio	202:242	dietary amylose/amylopectin (AM/AP) ratio	202:242	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	9	80	theme	other	1577:1581	arg1	diets					1583:1587	the other diets	1573:1587	the other diets	1573:1587	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	9	81	theme	alanine	1649:1655	arg1	aminotransferase					1657:1672	alanine aminotransferase	1649:1672	alanine aminotransferase	1649:1672	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	0	82	from	responses	132:140	arg1	obscurus					178:185	juvenile obscure puffer Takifugu obscurus	145:185	juvenile obscure puffer Takifugu obscurus	145:185	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	8	83	theme	AM/AP	1461:1465	arg1	ratio					1467:1471	dietary AM/AP ratio	1453:1471	dietary AM/AP ratio	1453:1471	Activities of liver and intestinal amylase and intestinal lipase, and starch digestibility were negatively correlated with dietary AM/AP ratio.
24710601	1	84	theme	ratio	238:242	arg1	effect					192:197	The effect	188:197	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses	188:360	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	11	85	theme	fish	2049:2052	arg1	value					2040:2044	Postprandial plasma glucose and triglyceride peak value	1990:2044	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13	1990:2067	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	10	86	theme	Equal	1732:1736	arg1	values					1738:1743	Equal values	1732:1743	Equal values	1732:1743	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	2	87	theme	equal	533:537	arg1	starch					566:571	250 g kg(-1) starch	553:571	250 g kg(-1) starch	553:571	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	2	87	theme	equal	533:537	arg1	level					546:550	an equal starch level	530:550	an equal starch level (250 g kg(-1) starch)	530:572	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	9	88	theme	aminotransferase	1688:1703	arg1	activities					1635:1644	activities	1635:1644	activities of alanine aminotransferase and aspartate aminotransferase	1635:1703	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	9	88	theme	aminotransferase	1688:1703	arg1	concentration					1617:1629	plasma urea nitrogen concentration	1596:1629	plasma urea nitrogen concentration	1596:1629	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	1	89	theme	feed	255:258	arg1	utilization					260:270	feed utilization	255:270	feed utilization	255:270	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	10	90	theme	lipase	1932:1937	arg1	composition					1825:1835	whole body and muscle composition	1803:1835	composition	1825:1835	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	90	theme	lipase	1932:1937	arg1	factor					1795:1800	condition factor	1785:1800	condition factor	1785:1800	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	90	theme	lipase	1932:1937	arg1	concentrations					1898:1911	plasma high-density and low-density lipoprotein cholesterol concentrations	1838:1911	plasma high-density and low-density lipoprotein cholesterol concentrations	1838:1911	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	90	theme	lipase	1932:1937	arg1	index					1775:1779	viscerosomatic index	1760:1779	viscerosomatic index	1760:1779	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	90	theme	lipase	1932:1937	arg1	body					1809:1812	whole body and muscle composition	1803:1835	body	1809:1812	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	90	theme	lipase	1932:1937	arg1	activities					1918:1927	activities	1918:1927	activities	1918:1927	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	90	theme	lipase	1932:1937	arg1	glucose-6-phosphatase					1958:1978	glucose-6-phosphatase	1958:1978	glucose-6-phosphatase in liver	1958:1987	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	11	91	theme	peak	2128:2131	arg1	later					2160:2164	later	2160:2164	later	2160:2164	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	11	91	theme	peak	2128:2131	arg1	time					2133:2136	the peak time	2124:2136	the peak time of plasma glucose	2124:2154	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	13	92	theme	weight	2380:2385	arg1	gain					2387:2390	weight gain	2380:2390	weight gain against dietary AM/AP ratio	2380:2418	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	14	93	theme	digestive	2632:2640	arg1	enzyme					2642:2647	digestive enzyme	2632:2647	digestive enzyme	2632:2647	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	14	93	theme	digestive	2632:2640	arg1	utilization					2595:2605	utilization	2595:2605	utilization	2595:2605	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	4	94	theme	postprandial	798:809	arg1	test					826:829	a postprandial blood response test	796:829	a postprandial blood response test	796:829	After the growth trial, a postprandial blood response test was carried out.
24710601	6	95	theme	fructose-1,6-bisphosphatase	1067:1093	arg1	activities					1095:1104	gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities	1032:1104	gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities	1032:1104	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	7	96	theme	trypsin	1231:1237	arg1	Activities					1196:1205	Activities	1196:1205	Activities of liver and intestinal trypsin in fish fed diet 3/22 and diet 6/19	1196:1273	Activities of liver and intestinal trypsin in fish fed diet 3/22 and diet 6/19 were higher than in fish fed diet 9/16 and diet 12/13.
24710601	0	97	theme	metabolic	122:130	arg1	responses					132:140	postprandial metabolic responses	109:140	postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus	109:185	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	6	98	theme	cholesterol	967:977	arg1	concentration					979:991	plasma total cholesterol concentration	954:991	plasma total cholesterol concentration	954:991	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	6	99	theme	plasma	954:959	arg1	concentration					979:991	plasma total cholesterol concentration	954:991	plasma total cholesterol concentration	954:991	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	9	100	dep	diet	1483:1486	arg1	6/19					1502:1505	6/19	1502:1505	6/19	1502:1505	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	1	101	theme	obscure	388:394	arg1	puffer					396:401	juvenile obscure puffer	379:401	juvenile obscure puffer	379:401	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	1	101	theme	obscure	388:394	arg1	obscurus					413:420	Takifugu obscurus	404:420	Takifugu obscurus	404:420	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	13	102	theme	obscure	2475:2481	arg1	puffer					2483:2488	obscure puffer	2475:2488	obscure puffer	2475:2488	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	1	103	theme	amylose/amylopectin	210:228	arg1	ratio					238:242	dietary amylose/amylopectin (AM/AP) ratio	202:242	dietary amylose/amylopectin (AM/AP) ratio	202:242	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	14	104	theme	glucose	2668:2674	arg1	activities					2693:2702	hepatic glucose metabolic enzyme activities	2660:2702	utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer	2595:2729	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	10	105	theme	high-density	1845:1856	arg1	plasma					1838:1843	plasma high-density	1838:1856	plasma high-density	1838:1856	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	6	106	theme	pyruvate	1047:1054	arg1	kinase					1056:1061	pyruvate kinase	1047:1061	pyruvate kinase	1047:1061	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	11	107	theme	low-amylose	2101:2111	arg1	diets					2113:2117	the low-amylose diets	2097:2117	the low-amylose diets	2097:2117	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	0	108	theme	obscure	154:160	arg1	obscurus					178:185	juvenile obscure puffer Takifugu obscurus	145:185	juvenile obscure puffer Takifugu obscurus	145:185	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	5	109	theme	efficiency	892:901	arg1	ratio					926:930	best growth, feed efficiency and protein efficiency ratio	874:930	best growth, feed efficiency and protein efficiency ratio	874:930	Fish fed diet 6/19 showed best growth, feed efficiency and protein efficiency ratio.
24710601	9	110	theme	higher	1514:1519	arg1	concentration					1545:1557	higher plasma total amino acid concentration	1514:1557	higher plasma total amino acid concentration than fish	1514:1567	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	6	111	theme	gluconokinase	1032:1044	arg1	activities					1095:1104	gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities	1032:1104	gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities	1032:1104	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	2	112	theme	ratio	595:599	arg1	diets					601:605	different AM/AP ratio diets	579:605	different AM/AP ratio diets	579:605	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	2	113	contain	containing	519:528	arg2	starch					566:571	250 g kg(-1) starch	553:571	250 g kg(-1) starch	553:571	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	2	113	contain	containing	519:528	arg2	level					546:550	an equal starch level	530:550	an equal starch level (250 g kg(-1) starch)	530:572	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	2	113	contain	containing	519:528	arg1	diets					513:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets	423:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13	423:641	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	10	114	theme	condition	1785:1793	arg1	factor					1795:1800	condition factor	1785:1800	condition factor	1785:1800	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	6	115	theme	AM/AP	1137:1141	arg1	diet					1143:1146	highest AM/AP diet	1129:1146	highest AM/AP diet (12/13)	1129:1154	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	6	115	theme	AM/AP	1137:1141	arg1	12/13					1149:1153	12/13	1149:1153	12/13	1149:1153	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	6	116	theme	total	961:965	arg1	concentration					979:991	plasma total cholesterol concentration	954:991	plasma total cholesterol concentration	954:991	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	2	117	theme	crude	500:504	arg1	lipid					506:510	90 g kg(-1) crude lipid	488:510	90 g kg(-1) crude lipid	488:510	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	0	118	from	enzymes	96:102	arg1	obscurus					178:185	juvenile obscure puffer Takifugu obscurus	145:185	juvenile obscure puffer Takifugu obscurus	145:185	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	14	119	theme	growth	2567:2572	arg1	performance					2574:2584	growth performance	2567:2584	growth performance	2567:2584	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	10	120	theme	muscle	1818:1823	arg1	composition					1825:1835	whole body and muscle composition	1803:1835	composition	1825:1835	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	0	121	theme	feed	68:71	arg1	utilization					73:83	feed utilization	68:83	feed utilization	68:83	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	5	122	theme	best	874:877	arg1	ratio					926:930	best growth, feed efficiency and protein efficiency ratio	874:930	best growth, feed efficiency and protein efficiency ratio	874:930	Fish fed diet 6/19 showed best growth, feed efficiency and protein efficiency ratio.
24710601	1	123	from	effect	192:197	arg1	activities					290:299	digestive enzyme activities	273:299	digestive enzyme activities	273:299	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	1	123	from	effect	192:197	arg1	parameters					309:318	plasma parameters	302:318	plasma parameters	302:318	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	1	123	from	effect	192:197	arg1	responses					352:360	postprandial blood glucose responses	325:360	postprandial blood glucose responses	325:360	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	1	123	from	effect	192:197	arg1	growth					247:252	growth	247:252	growth	247:252	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	1	123	from	effect	192:197	arg1	utilization					260:270	feed utilization	255:270	feed utilization	255:270	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	11	124	theme	plasma	2141:2146	arg1	glucose					2148:2154	plasma glucose	2141:2154	plasma glucose	2141:2154	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	9	125	theme	acid	1540:1543	arg1	concentration					1545:1557	higher plasma total amino acid concentration	1514:1557	higher plasma total amino acid concentration than fish	1514:1567	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	6	126	theme	lipid	1013:1017	arg1	content					1019:1025	liver glycogen and lipid content	994:1025	content	1019:1025	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	3	127	theme	experimental	665:676	arg1	diet					678:681	Each experimental diet	660:681	Each experimental diet	660:681	Each experimental diet was fed to triplicate groups (25 fish per tank), twice daily during a period of 60 days.
24710601	1	128	theme	postprandial	325:336	arg1	responses					352:360	postprandial blood glucose responses	325:360	postprandial blood glucose responses	325:360	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	11	129	theme	glucose	2148:2154	arg1	later					2160:2164	later	2160:2164	later	2160:2164	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	11	129	theme	glucose	2148:2154	arg1	time					2133:2136	the peak time	2124:2136	the peak time of plasma glucose	2124:2154	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	2	130	theme	crude	457:461	arg1	protein					463:469	430 g kg(-1) crude protein	444:469	430 g kg(-1) crude protein	444:469	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	1	131	theme	juvenile	379:386	arg1	puffer					396:401	juvenile obscure puffer	379:401	juvenile obscure puffer	379:401	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	1	131	theme	juvenile	379:386	arg1	obscurus					413:420	Takifugu obscurus	404:420	Takifugu obscurus	404:420	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	6	132	theme	low-amylose	1177:1187	arg1	diets					1189:1193	the low-amylose diets	1173:1193	the low-amylose diets	1173:1193	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	1	133	theme	glucose	344:350	arg1	responses					352:360	postprandial blood glucose responses	325:360	postprandial blood glucose responses	325:360	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	0	134	theme	puffer	162:167	arg1	obscurus					178:185	juvenile obscure puffer Takifugu obscurus	145:185	juvenile obscure puffer Takifugu obscurus	145:185	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	0	135	from	performance	55:65	arg1	obscurus					178:185	juvenile obscure puffer Takifugu obscurus	145:185	juvenile obscure puffer Takifugu obscurus	145:185	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	9	136	dep	showed	1507:1512	arg1	fed					1569:1571	fed	1569:1571	fed	1569:1571	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	5	137	theme	protein	907:913	arg1	efficiency					915:924	protein efficiency	907:924	protein efficiency	907:924	Fish fed diet 6/19 showed best growth, feed efficiency and protein efficiency ratio.
24710601	10	138	from	factor	1795:1800	arg1	liver					1983:1987	liver	1983:1987	liver	1983:1987	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	139	theme	lipoprotein	1874:1884	arg1	cholesterol					1886:1896	low-density lipoprotein cholesterol	1862:1896	low-density lipoprotein cholesterol	1862:1896	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	2	140	theme	isolipidic	476:485	arg1	diets					513:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets	423:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13	423:641	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	2	141	theme	different	579:587	arg1	diets					601:605	different AM/AP ratio diets	579:605	different AM/AP ratio diets	579:605	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	13	142	theme	gain	2387:2390	arg1	analysis					2368:2375	regression analysis	2357:2375	regression analysis of weight gain against dietary AM/AP ratio	2357:2418	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	14	143	theme	dietary	2534:2540	arg1	ratio					2548:2552	dietary AM/AP ratio	2534:2552	dietary AM/AP ratio	2534:2552	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	11	144	theme	other	2187:2191	arg1	diets					2193:2197	the other diets	2183:2197	the other diets	2183:2197	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	0	145	theme	amylose/amylopectin	19:37	arg1	ratio					39:43	dietary amylose/amylopectin ratio	11:43	dietary amylose/amylopectin ratio	11:43	Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.
24710601	1	146	theme	digestive	273:281	arg1	activities					290:299	digestive enzyme activities	273:299	digestive enzyme activities	273:299	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	2	147	theme	isonitrogenous	428:441	arg1	diets					513:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets	423:517	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13	423:641	Five isonitrogenous (430 g kg(-1) crude protein) and isolipidic (90 g kg(-1) crude lipid) diets containing an equal starch level (250 g kg(-1) starch) with different AM/AP ratio diets of 0/25, 3/22, 6/19, 9/16 and 12/13 were formulated.
24710601	10	148	from	activities	1918:1927	arg1	liver					1983:1987	liver	1983:1987	liver	1983:1987	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	9	149	theme	opposite	1716:1723	arg1	trend					1725:1729	the opposite trend	1712:1729	the opposite trend	1712:1729	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	13	150	theme	regression	2357:2366	arg1	analysis					2368:2375	regression analysis	2357:2375	regression analysis of weight gain against dietary AM/AP ratio	2357:2418	According to regression analysis of weight gain against dietary AM/AP ratio, the optimum dietary AM/AP ratio for maximum growth of obscure puffer was 0.25.
24710601	10	151	from	composition	1825:1835	arg1	liver					1983:1987	liver	1983:1987	liver	1983:1987	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	152	theme	low-density	1862:1872	arg1	cholesterol					1886:1896	low-density lipoprotein cholesterol	1862:1896	low-density lipoprotein cholesterol	1862:1896	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	14	153	theme	present	2504:2510	arg1	result					2512:2517	The present result	2500:2517	The present result	2500:2517	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	4	154	theme	response	817:824	arg1	test					826:829	a postprandial blood response test	796:829	a postprandial blood response test	796:829	After the growth trial, a postprandial blood response test was carried out.
24710601	8	155	theme	amylase	1365:1371	arg1	Activities					1330:1339	Activities	1330:1339	Activities of liver and intestinal amylase and intestinal lipase	1330:1393	Activities of liver and intestinal amylase and intestinal lipase, and starch digestibility were negatively correlated with dietary AM/AP ratio.
24710601	8	155	theme	amylase	1365:1371	arg1	digestibility					1407:1419	starch digestibility	1400:1419	starch digestibility	1400:1419	Activities of liver and intestinal amylase and intestinal lipase, and starch digestibility were negatively correlated with dietary AM/AP ratio.
24710601	12	156	theme	Plasma	2200:2205	arg1	glucose					2207:2213	Plasma glucose and triglyceride concentrations	2200:2245	glucose	2207:2213	Plasma glucose and triglyceride concentrations showed a significant difference at 2 and 4 h after a meal and varied between dietary treatments.
24710601	4	157	theme	growth	782:787	arg1	trial					789:793	the growth trial	778:793	the growth trial	778:793	After the growth trial, a postprandial blood response test was carried out.
24710601	1	158	theme	plasma	302:307	arg1	parameters					309:318	plasma parameters	302:318	plasma parameters	302:318	The effect of dietary amylose/amylopectin (AM/AP) ratio on growth, feed utilization, digestive enzyme activities, plasma parameters, and postprandial blood glucose responses was evaluated in juvenile obscure puffer, Takifugu obscurus.
24710601	5	159	theme	Fish	848:851	arg1	6/19					862:865	Fish fed diet 6/19	848:865	Fish fed diet 6/19	848:865	Fish fed diet 6/19 showed best growth, feed efficiency and protein efficiency ratio.
24710601	11	160	theme	peak	2035:2038	arg1	value					2040:2044	Postprandial plasma glucose and triglyceride peak value	1990:2044	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13	1990:2067	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
24710601	8	161	theme	intestinal	1377:1386	arg1	lipase					1388:1393	intestinal lipase	1377:1393	intestinal lipase	1377:1393	Activities of liver and intestinal amylase and intestinal lipase, and starch digestibility were negatively correlated with dietary AM/AP ratio.
24710601	14	162	theme	hepatic	2660:2666	arg1	activities					2693:2702	hepatic glucose metabolic enzyme activities	2660:2702	utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer	2595:2729	The present result indicates that dietary AM/AP ratio could affect growth performance and feed utilization, some plasma parameters, digestive enzyme as well as hepatic glucose metabolic enzyme activities in juvenile obscure puffer.
24710601	8	163	theme	dietary	1453:1459	arg1	ratio					1467:1471	dietary AM/AP ratio	1453:1471	dietary AM/AP ratio	1453:1471	Activities of liver and intestinal amylase and intestinal lipase, and starch digestibility were negatively correlated with dietary AM/AP ratio.
24710601	12	164	theme	dietary	2324:2330	arg1	treatments					2332:2341	dietary treatments	2324:2341	dietary treatments	2324:2341	Plasma glucose and triglyceride concentrations showed a significant difference at 2 and 4 h after a meal and varied between dietary treatments.
24710601	6	165	theme	Hepatosomatic	933:945	arg1	index					947:951	Hepatosomatic index	933:951	Hepatosomatic index	933:951	Hepatosomatic index, plasma total cholesterol concentration, liver glycogen and lipid content, and gluconokinase, pyruvate kinase and fructose-1,6-bisphosphatase activities were lower in fish fed highest AM/AP diet (12/13) than in fish fed the low-amylose diets.
24710601	10	166	theme	cholesterol	1886:1896	arg1	concentrations					1898:1911	plasma high-density and low-density lipoprotein cholesterol concentrations	1838:1911	plasma high-density and low-density lipoprotein cholesterol concentrations	1838:1911	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	10	167	from	glucose-6-phosphatase	1958:1978	arg1	liver					1983:1987	liver	1983:1987	liver	1983:1987	Equal values were found for viscerosomatic index and condition factor, whole body and muscle composition, plasma high-density and low-density lipoprotein cholesterol concentrations, and activities of lipase and hexokinase and glucose-6-phosphatase in liver.
24710601	9	168	theme	aminotransferase	1657:1672	arg1	activities					1635:1644	activities	1635:1644	activities of alanine aminotransferase and aspartate aminotransferase	1635:1703	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	9	168	theme	aminotransferase	1657:1672	arg1	concentration					1617:1629	plasma urea nitrogen concentration	1596:1629	plasma urea nitrogen concentration	1596:1629	Fish fed diet 3/22 and diet 6/19 showed higher plasma total amino acid concentration than fish fed the other diets, while plasma urea nitrogen concentration and activities of alanine aminotransferase and aspartate aminotransferase showed the opposite trend.
24710601	8	169	theme	starch	1400:1405	arg1	digestibility					1407:1419	starch digestibility	1400:1419	starch digestibility	1400:1419	Activities of liver and intestinal amylase and intestinal lipase, and starch digestibility were negatively correlated with dietary AM/AP ratio.
24710601	11	170	theme	glucose	2010:2016	arg1	value					2040:2044	Postprandial plasma glucose and triglyceride peak value	1990:2044	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13	1990:2067	Postprandial plasma glucose and triglyceride peak value of fish fed diet 12/13 were lower than in fish fed the low-amylose diets, and the peak time of plasma glucose was later than in fish fed the other diets.
27864063	7	0	theme	scaffold	1284:1291	arg1	application					1264:1274	topical application	1256:1274	topical application of Q-CF scaffold on open excision type of wounds	1256:1323	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	4	1	theme	wound	884:888	arg1	material					899:906	a wound dressing material	882:906	a wound dressing material	882:906	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	6	2	theme	tissue	1147:1152	arg1	applications					1166:1177	tissue engineering applications	1147:1177	tissue engineering applications	1147:1177	Biocompatibility of Q-CF scaffold was assessed using MTT assay, which elucidated its non-toxic property and excellent suitability for tissue engineering applications.
27864063	1	3	theme	dressing	269:276	arg1	application					278:288	wound dressing application	263:288	wound dressing application	263:288	The present study efforts at fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application and aims at investigating their physicochemical properties.
27864063	6	4	theme	non-toxic	1098:1106	arg1	property					1108:1115	its non-toxic property	1094:1115	its non-toxic property	1094:1115	Biocompatibility of Q-CF scaffold was assessed using MTT assay, which elucidated its non-toxic property and excellent suitability for tissue engineering applications.
27864063	7	5	theme	In	1180:1181	arg1	healing					1194:1200	In vivo wound healing	1180:1200	In vivo wound healing experiments performed using albino rats	1180:1240	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	7	6	theme	wound	1188:1192	arg1	healing					1194:1200	In vivo wound healing	1180:1200	In vivo wound healing experiments performed using albino rats	1180:1240	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	6	7	theme	engineering	1154:1164	arg1	applications					1166:1177	tissue engineering applications	1147:1177	tissue engineering applications	1147:1177	Biocompatibility of Q-CF scaffold was assessed using MTT assay, which elucidated its non-toxic property and excellent suitability for tissue engineering applications.
27864063	2	8	theme	acidic	387:392	arg1	solution					394:401	acidic solution	387:401	acidic solution of chitosan	387:413	CF scaffolds were prepared by mixing acidic solution of chitosan with an alkaline solution of fibrin, to which quercetin (Q) was added, homogenized and lyophilized obtain Q-CF scaffold.
27864063	8	9	theme	promising	1442:1450	arg1	material					1467:1474	a promising wound dressing material	1440:1474	a promising wound dressing material	1440:1474	These results suggest that Q-CF scaffold could serve as a promising wound dressing material.
27864063	8	9	theme	promising	1442:1450	arg1	scaffold					1416:1423	Q-CF scaffold	1411:1423	Q-CF scaffold	1411:1423	These results suggest that Q-CF scaffold could serve as a promising wound dressing material.
27864063	7	10	theme	healing	1375:1381	arg1	process					1358:1364	the process	1354:1364	the process of wound healing	1354:1381	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	0	11	dep	in	132:133	arg1	vivo					135:138	vivo	135:138	vivo	135:138	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	4	12	theme	temperature	756:766	arg1	range					768:772	the temperature range	752:772	the temperature range of 255-400°C. Q-CF scaffold	752:800	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	6	13	theme	scaffold	1038:1045	arg1	Biocompatibility					1013:1028	Biocompatibility	1013:1028	Biocompatibility of Q-CF scaffold	1013:1045	Biocompatibility of Q-CF scaffold was assessed using MTT assay, which elucidated its non-toxic property and excellent suitability for tissue engineering applications.
27864063	7	14	theme	Q-CF	1279:1282	arg1	scaffold					1284:1291	Q-CF scaffold	1279:1291	Q-CF scaffold	1279:1291	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	7	15	from	application	1264:1274	arg1	type					1310:1313	open excision type	1296:1313	open excision type of wounds	1296:1323	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	4	16	theme	dressing	890:897	arg1	material					899:906	a wound dressing material	882:906	a wound dressing material	882:906	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	7	17	theme	healing	1194:1200	arg1	experiments					1202:1212	In vivo wound healing experiments	1180:1212	In vivo wound healing experiments performed using albino rats	1180:1240	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	6	18	theme	Q-CF	1033:1036	arg1	scaffold					1038:1045	Q-CF scaffold	1033:1045	Q-CF scaffold	1033:1045	Biocompatibility of Q-CF scaffold was assessed using MTT assay, which elucidated its non-toxic property and excellent suitability for tissue engineering applications.
27864063	0	19	theme	in	132:133	arg1	analysis					140:147	in vivo analysis	132:147	in vivo analysis	132:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	19	theme	in	132:133	arg1	materials					86:94	potential wound dressing materials	61:94	potential wound dressing materials - Fabrication, characterization and in vivo analysis	61:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	5	20	theme	good	934:937	arg1	activity					952:959	good bactericidal activity	934:959	good bactericidal activity	934:959	Q-CF scaffolds exhibited good bactericidal activity against Escherichia coli and Staphylococcus aureus.
27864063	4	21	theme	mechanical	858:867	arg1	strength					869:876	an ideal mechanical strength	849:876	an ideal mechanical strength for a wound dressing material	849:906	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	4	21	theme	mechanical	858:867	arg1	1.45MPa					840:846	1.45MPa	840:846	1.45MPa	840:846	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	4	22	theme	scaffold	793:800	arg1	range					768:772	the temperature range	752:772	the temperature range of 255-400°C. Q-CF scaffold	752:800	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	4	23	theme	saccharide	693:702	arg1	rings					704:708	saccharide rings	693:708	saccharide rings	693:708	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	1	24	theme	fabricating	179:189	arg1	scaffolds					222:230	fabricating chitosan-fibrin composite (CF) scaffolds	179:230	fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application	179:288	The present study efforts at fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application and aims at investigating their physicochemical properties.
27864063	2	25	theme	Q-CF	521:524	arg1	scaffold					526:533	Q-CF scaffold	521:533	Q-CF scaffold	521:533	CF scaffolds were prepared by mixing acidic solution of chitosan with an alkaline solution of fibrin, to which quercetin (Q) was added, homogenized and lyophilized obtain Q-CF scaffold.
27864063	4	26	theme	ideal	852:856	arg1	strength					869:876	an ideal mechanical strength	849:876	an ideal mechanical strength for a wound dressing material	849:906	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	4	26	theme	ideal	852:856	arg1	1.45MPa					840:846	1.45MPa	840:846	1.45MPa	840:846	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	4	27	from	range	768:772	arg1	acids					720:724	amino acids	714:724	amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold	714:800	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	4	28	theme	Q-CF	788:791	arg1	scaffold					793:800	255-400°C. Q-CF scaffold	777:800	255-400°C. Q-CF scaffold	777:800	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	2	29	theme	alkaline	423:430	arg1	solution					432:439	an alkaline solution	420:439	an alkaline solution	420:439	CF scaffolds were prepared by mixing acidic solution of chitosan with an alkaline solution of fibrin, to which quercetin (Q) was added, homogenized and lyophilized obtain Q-CF scaffold.
27864063	1	30	theme	composite	207:215	arg1	scaffolds					222:230	fabricating chitosan-fibrin composite (CF) scaffolds	179:230	fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application	179:288	The present study efforts at fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application and aims at investigating their physicochemical properties.
27864063	7	31	dep	In	1180:1181	arg1	vivo					1183:1186	vivo	1183:1186	vivo	1183:1186	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	2	32	theme	CF	350:351	arg1	scaffolds					353:361	CF scaffolds	350:361	CF scaffolds	350:361	CF scaffolds were prepared by mixing acidic solution of chitosan with an alkaline solution of fibrin, to which quercetin (Q) was added, homogenized and lyophilized obtain Q-CF scaffold.
27864063	4	33	theme	255-400°C.	777:786	arg1	scaffold					793:800	255-400°C. Q-CF scaffold	777:800	255-400°C. Q-CF scaffold	777:800	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	3	34	used	used	554:557	arg2	spectra					541:547	FTIR spectra	536:547	FTIR spectra	536:547	FTIR spectra were used to determine the interactions between the functional groups of quercetin and CF scaffolds.
27864063	0	35	theme	composite	38:46	arg1	materials					86:94	potential wound dressing materials	61:94	potential wound dressing materials - Fabrication, characterization and in vivo analysis	61:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	35	theme	composite	38:46	arg1	scaffolds					48:56	chitosan-fibrin composite scaffolds	22:56	chitosan-fibrin composite scaffolds	22:56	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	6	36	theme	excellent	1121:1129	arg1	suitability					1131:1141	excellent suitability	1121:1141	excellent suitability for tissue engineering applications	1121:1177	Biocompatibility of Q-CF scaffold was assessed using MTT assay, which elucidated its non-toxic property and excellent suitability for tissue engineering applications.
27864063	0	37	dep	materials	86:94	arg1	characterization					111:126	characterization	111:126	characterization	111:126	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	37	dep	materials	86:94	arg1	Fabrication					98:108	Fabrication	98:108	Fabrication	98:108	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	37	dep	materials	86:94	arg1	analysis					140:147	in vivo analysis	132:147	in vivo analysis	132:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	37	dep	materials	86:94	arg1	materials					86:94	potential wound dressing materials	61:94	potential wound dressing materials - Fabrication, characterization and in vivo analysis	61:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	1	38	theme	CF	218:219	arg1	scaffolds					222:230	fabricating chitosan-fibrin composite (CF) scaffolds	179:230	fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application	179:288	The present study efforts at fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application and aims at investigating their physicochemical properties.
27864063	0	39	theme	chitosan-fibrin	22:36	arg1	materials					86:94	potential wound dressing materials	61:94	potential wound dressing materials - Fabrication, characterization and in vivo analysis	61:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	39	theme	chitosan-fibrin	22:36	arg1	scaffolds					48:56	chitosan-fibrin composite scaffolds	22:56	chitosan-fibrin composite scaffolds	22:56	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	4	40	theme	amino	714:718	arg1	acids					720:724	amino acids	714:724	amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold	714:800	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	1	41	theme	present	154:160	arg1	study					162:166	The present study	150:166	The present study	150:166	The present study efforts at fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application and aims at investigating their physicochemical properties.
27864063	1	42	theme	physicochemical	322:336	arg1	properties					338:347	their physicochemical properties	316:347	their physicochemical properties	316:347	The present study efforts at fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application and aims at investigating their physicochemical properties.
27864063	7	43	theme	wounds	1318:1323	arg1	type					1310:1313	open excision type	1296:1313	open excision type of wounds	1296:1323	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	4	44	theme	1.45MPa	840:846	arg1	strength					828:835	maximum tensile strength	812:835	maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material	812:906	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	2	45	theme	fibrin	444:449	arg1	solution					432:439	an alkaline solution	420:439	an alkaline solution	420:439	CF scaffolds were prepared by mixing acidic solution of chitosan with an alkaline solution of fibrin, to which quercetin (Q) was added, homogenized and lyophilized obtain Q-CF scaffold.
27864063	7	46	theme	albino	1230:1235	arg1	rats					1237:1240	albino rats	1230:1240	albino rats	1230:1240	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	7	47	theme	wound	1369:1373	arg1	healing					1375:1381	wound healing	1369:1381	wound healing	1369:1381	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	0	48	theme	wound	71:75	arg1	characterization					111:126	characterization	111:126	characterization	111:126	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	48	theme	wound	71:75	arg1	Fabrication					98:108	Fabrication	98:108	Fabrication	98:108	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	48	theme	wound	71:75	arg1	materials					86:94	potential wound dressing materials	61:94	potential wound dressing materials - Fabrication, characterization and in vivo analysis	61:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	48	theme	wound	71:75	arg1	scaffolds					48:56	chitosan-fibrin composite scaffolds	22:56	chitosan-fibrin composite scaffolds	22:56	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	48	theme	wound	71:75	arg1	analysis					140:147	in vivo analysis	132:147	in vivo analysis	132:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	7	49	theme	excision	1301:1308	arg1	type					1310:1313	open excision type	1296:1313	open excision type of wounds	1296:1323	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	4	50	theme	chitosan	729:736	arg1	acids					720:724	amino acids	714:724	amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold	714:800	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	0	51	theme	potential	61:69	arg1	characterization					111:126	characterization	111:126	characterization	111:126	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	51	theme	potential	61:69	arg1	Fabrication					98:108	Fabrication	98:108	Fabrication	98:108	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	51	theme	potential	61:69	arg1	materials					86:94	potential wound dressing materials	61:94	potential wound dressing materials - Fabrication, characterization and in vivo analysis	61:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	51	theme	potential	61:69	arg1	scaffolds					48:56	chitosan-fibrin composite scaffolds	22:56	chitosan-fibrin composite scaffolds	22:56	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	51	theme	potential	61:69	arg1	analysis					140:147	in vivo analysis	132:147	in vivo analysis	132:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	3	52	theme	functional	601:610	arg1	groups					612:617	the functional groups	597:617	the functional groups of quercetin and CF scaffolds	597:647	FTIR spectra were used to determine the interactions between the functional groups of quercetin and CF scaffolds.
27864063	3	52	theme	functional	601:610	arg1	scaffolds					639:647	quercetin and CF scaffolds	622:647	quercetin and CF scaffolds	622:647	FTIR spectra were used to determine the interactions between the functional groups of quercetin and CF scaffolds.
27864063	5	53	theme	bactericidal	939:950	arg1	activity					952:959	good bactericidal activity	934:959	good bactericidal activity	934:959	Q-CF scaffolds exhibited good bactericidal activity against Escherichia coli and Staphylococcus aureus.
27864063	4	54	theme	maximum	812:818	arg1	strength					828:835	maximum tensile strength	812:835	maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material	812:906	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	3	55	theme	CF	636:637	arg1	scaffolds					639:647	quercetin and CF scaffolds	622:647	quercetin and CF scaffolds	622:647	FTIR spectra were used to determine the interactions between the functional groups of quercetin and CF scaffolds.
27864063	8	56	theme	dressing	1458:1465	arg1	material					1467:1474	a promising wound dressing material	1440:1474	a promising wound dressing material	1440:1474	These results suggest that Q-CF scaffold could serve as a promising wound dressing material.
27864063	8	56	theme	dressing	1458:1465	arg1	scaffold					1416:1423	Q-CF scaffold	1411:1423	Q-CF scaffold	1411:1423	These results suggest that Q-CF scaffold could serve as a promising wound dressing material.
27864063	4	57	theme	fibrin	742:747	arg1	acids					720:724	amino acids	714:724	amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold	714:800	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	8	58	theme	wound	1452:1456	arg1	material					1467:1474	a promising wound dressing material	1440:1474	a promising wound dressing material	1440:1474	These results suggest that Q-CF scaffold could serve as a promising wound dressing material.
27864063	8	58	theme	wound	1452:1456	arg1	scaffold					1416:1423	Q-CF scaffold	1411:1423	Q-CF scaffold	1411:1423	These results suggest that Q-CF scaffold could serve as a promising wound dressing material.
27864063	3	59	theme	scaffolds	639:647	arg1	groups					612:617	the functional groups	597:617	the functional groups of quercetin and CF scaffolds	597:647	FTIR spectra were used to determine the interactions between the functional groups of quercetin and CF scaffolds.
27864063	3	59	theme	scaffolds	639:647	arg1	scaffolds					639:647	quercetin and CF scaffolds	622:647	quercetin and CF scaffolds	622:647	FTIR spectra were used to determine the interactions between the functional groups of quercetin and CF scaffolds.
27864063	4	60	theme	rings	704:708	arg1	decomposition					676:688	the decomposition	672:688	the decomposition of saccharide rings	672:708	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	8	61	theme	Q-CF	1411:1414	arg1	scaffold					1416:1423	Q-CF scaffold	1411:1423	Q-CF scaffold	1411:1423	These results suggest that Q-CF scaffold could serve as a promising wound dressing material.
27864063	8	61	theme	Q-CF	1411:1414	arg1	material					1467:1474	a promising wound dressing material	1440:1474	a promising wound dressing material	1440:1474	These results suggest that Q-CF scaffold could serve as a promising wound dressing material.
27864063	7	62	theme	open	1296:1299	arg1	type					1310:1313	open excision type	1296:1313	open excision type of wounds	1296:1323	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	5	63	theme	Q-CF	909:912	arg1	scaffolds					914:922	Q-CF scaffolds	909:922	Q-CF scaffolds	909:922	Q-CF scaffolds exhibited good bactericidal activity against Escherichia coli and Staphylococcus aureus.
27864063	3	64	theme	quercetin	622:630	arg1	scaffolds					639:647	quercetin and CF scaffolds	622:647	quercetin and CF scaffolds	622:647	FTIR spectra were used to determine the interactions between the functional groups of quercetin and CF scaffolds.
27864063	4	65	theme	TGA	650:652	arg1	analysis					654:661	TGA analysis	650:661	TGA analysis	650:661	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	2	66	theme	chitosan	406:413	arg1	solution					394:401	acidic solution	387:401	acidic solution of chitosan	387:413	CF scaffolds were prepared by mixing acidic solution of chitosan with an alkaline solution of fibrin, to which quercetin (Q) was added, homogenized and lyophilized obtain Q-CF scaffold.
27864063	1	67	theme	chitosan-fibrin	191:205	arg1	scaffolds					222:230	fabricating chitosan-fibrin composite (CF) scaffolds	179:230	fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application	179:288	The present study efforts at fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application and aims at investigating their physicochemical properties.
27864063	6	68	theme	MTT	1066:1068	arg1	assay					1070:1074	MTT assay	1066:1074	MTT assay	1066:1074	Biocompatibility of Q-CF scaffold was assessed using MTT assay, which elucidated its non-toxic property and excellent suitability for tissue engineering applications.
27864063	0	69	theme	dressing	77:84	arg1	characterization					111:126	characterization	111:126	characterization	111:126	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	69	theme	dressing	77:84	arg1	Fabrication					98:108	Fabrication	98:108	Fabrication	98:108	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	69	theme	dressing	77:84	arg1	materials					86:94	potential wound dressing materials	61:94	potential wound dressing materials - Fabrication, characterization and in vivo analysis	61:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	69	theme	dressing	77:84	arg1	scaffolds					48:56	chitosan-fibrin composite scaffolds	22:56	chitosan-fibrin composite scaffolds	22:56	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	0	69	theme	dressing	77:84	arg1	analysis					140:147	in vivo analysis	132:147	in vivo analysis	132:147	Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.
27864063	7	70	theme	topical	1256:1262	arg1	application					1264:1274	topical application	1256:1274	topical application of Q-CF scaffold on open excision type of wounds	1256:1323	In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing.
27864063	4	71	theme	tensile	820:826	arg1	strength					828:835	maximum tensile strength	812:835	maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material	812:906	TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material.
27864063	3	72	theme	FTIR	536:539	arg1	spectra					541:547	FTIR spectra	536:547	FTIR spectra	536:547	FTIR spectra were used to determine the interactions between the functional groups of quercetin and CF scaffolds.
27864063	1	73	theme	wound	263:267	arg1	application					278:288	wound dressing application	263:288	wound dressing application	263:288	The present study efforts at fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application and aims at investigating their physicochemical properties.
26646703	0	0	theme	Relation	83:90	arg1	Study					12:16	Comparative Study	0:16	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.	0:142	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	0	1	with	Profile	30:36	arg1	Pigmentation					130:141	Contrasting Pigmentation	118:141	Contrasting Pigmentation	118:141	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	6	2	theme	stepwise	1079:1086	arg1	SLDA					1118:1121	SLDA	1118:1121	SLDA	1118:1121	Also, a stepwise linear discriminant analysis (SLDA) was developed in order to classify roselle cultivars.
26646703	6	2	theme	stepwise	1079:1086	arg1	analysis					1108:1115	a stepwise linear discriminant analysis	1077:1115	a stepwise linear discriminant analysis (SLDA)	1077:1122	Also, a stepwise linear discriminant analysis (SLDA) was developed in order to classify roselle cultivars.
26646703	0	3	theme	Color-composition	65:81	arg1	Relation					83:90	Color-composition Relation	65:90	Color-composition Relation	65:90	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	3	4	theme	benzoic	686:692	arg1	acids					717:721	benzoic, hibiscus and phenolic acids	686:721	benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside)	686:811	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	4	theme	benzoic	686:692	arg1	flavanols					664:672	flavanols	664:672	flavanols	664:672	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	2	5	dep	Mexico	465:470	arg1	Rosa					487:490	Rosa	487:490	Rosa	487:490	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	2	5	dep	Mexico	465:470	arg1	Sudan					480:484	Sudan	480:484	Sudan	480:484	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	2	5	dep	Mexico	465:470	arg1	Negra					473:477	Negra	473:477	Negra	473:477	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	2	5	dep	Mexico	465:470	arg1	Blanca					496:501	Blanca	496:501	Blanca	496:501	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	0	6	with	Relation	83:90	arg1	Pigmentation					130:141	Contrasting Pigmentation	118:141	Contrasting Pigmentation	118:141	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	3	7	used	used	626:629	arg2	methods					613:619	Chromatographic methods	597:619	Chromatographic methods	597:619	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	0	8	theme	Roselle	95:101	arg1	Cultivars					103:111	Roselle Cultivars	95:111	Roselle Cultivars	95:111	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	2	9	from	cultivars	452:460	arg1	Mexico					465:470	Mexico	465:470	Mexico	465:470	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	3	10	dep	anthocyanins	743:754	arg1	anthocyanins					743:754	two main anthocyanins	734:754	benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside)	686:811	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	10	dep	anthocyanins	743:754	arg1	3-sambubioside					766:779	cyanidin 3-sambubioside	757:779	cyanidin 3-sambubioside	757:779	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	10	dep	anthocyanins	743:754	arg1	3-sambubioside					797:810	delphinidin 3-sambubioside	785:810	delphinidin 3-sambubioside	785:810	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	7	11	theme	mathematical	1191:1202	arg1	tool					1232:1235	an important tool	1219:1235	an important tool to be used in colorimetric characterization of functional compounds used in food processing	1219:1327	The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.
26646703	7	11	theme	mathematical	1191:1202	arg1	model					1204:1208	The obtained mathematical model	1178:1208	The obtained mathematical model	1178:1208	The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.
26646703	2	12	theme	color-composition	565:581	arg1	relationship					583:594	the color-composition relationship	561:594	the color-composition relationship	561:594	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	6	13	theme	roselle	1159:1165	arg1	cultivars					1167:1175	roselle cultivars	1159:1175	roselle cultivars	1159:1175	Also, a stepwise linear discriminant analysis (SLDA) was developed in order to classify roselle cultivars.
26646703	1	14	theme	antioxidant	265:275	arg1	compounds					277:285	antioxidant compounds	265:285	antioxidant compounds	265:285	Roselle is a plant that accumulates anthocyanins significantly, hence its importance as food coloring and as a source of antioxidant compounds for human health.
26646703	7	15	theme	colorimetric	1251:1262	arg1	characterization					1264:1279	colorimetric characterization	1251:1279	colorimetric characterization of functional compounds used in food processing	1251:1327	The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.
26646703	2	16	theme	pigmentation	530:541	arg1	degrees					519:525	different degrees	509:525	different degrees of pigmentation	509:541	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	0	17	theme	Cultivars	103:111	arg1	Relation					83:90	Color-composition Relation	65:90	Color-composition Relation	65:90	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	0	17	theme	Cultivars	103:111	arg1	Profile					30:36	Phenolic Profile	21:36	Phenolic Profile	21:36	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	0	17	theme	Cultivars	103:111	arg1	Capacity					51:58	Antioxidant Capacity	39:58	Antioxidant Capacity	39:58	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	3	18	theme	phenolic	708:715	arg1	acids					717:721	benzoic, hibiscus and phenolic acids	686:721	benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside)	686:811	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	18	theme	phenolic	708:715	arg1	flavanols					664:672	flavanols	664:672	flavanols	664:672	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	2	19	theme	extracts	399:406	arg1	composition					348:358	phenolic composition	339:358	phenolic composition	339:358	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	2	19	theme	extracts	399:406	arg1	capacity					376:383	antioxidant capacity	364:383	antioxidant capacity	364:383	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	3	20	theme	main	738:741	arg1	anthocyanins					743:754	two main anthocyanins	734:754	benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside)	686:811	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	20	theme	main	738:741	arg1	3-sambubioside					766:779	cyanidin 3-sambubioside	757:779	cyanidin 3-sambubioside	757:779	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	20	theme	main	738:741	arg1	3-sambubioside					797:810	delphinidin 3-sambubioside	785:810	delphinidin 3-sambubioside	785:810	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	20	theme	main	738:741	arg1	flavanols					664:672	flavanols	664:672	flavanols	664:672	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	2	21	theme	methanolic	388:397	arg1	extracts					399:406	methanolic extracts	388:406	methanolic extracts	388:406	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	0	22	theme	Comparative	0:10	arg1	Study					12:16	Comparative Study	0:16	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.	0:142	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	1	23	theme	compounds	277:285	arg1	source					255:260	a source	253:260	a source of antioxidant compounds for human health	253:302	Roselle is a plant that accumulates anthocyanins significantly, hence its importance as food coloring and as a source of antioxidant compounds for human health.
26646703	4	24	theme	ABTS	856:859	arg1	assays					870:875	ABTS and FRAP assays	856:875	ABTS and FRAP assays	856:875	The antioxidant capacity was evaluated by ABTS and FRAP assays.
26646703	4	25	theme	FRAP	865:868	arg1	assays					870:875	ABTS and FRAP assays	856:875	ABTS and FRAP assays	856:875	The antioxidant capacity was evaluated by ABTS and FRAP assays.
26646703	0	26	theme	Phenolic	21:28	arg1	Profile					30:36	Phenolic Profile	21:36	Phenolic Profile	21:36	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	5	27	theme	color-composition	951:967	arg1	relationship					969:980	the color-composition relationship	947:980	the color-composition relationship	947:980	Tristimulus colorimetry showed to be a useful technique to determine the color-composition relationship, leading to equations that allowed to predict anthocyanin content of roselle (R > 0.84).
26646703	5	28	theme	anthocyanin	1028:1038	arg1	content					1040:1046	anthocyanin content	1028:1046	anthocyanin content of roselle	1028:1057	Tristimulus colorimetry showed to be a useful technique to determine the color-composition relationship, leading to equations that allowed to predict anthocyanin content of roselle (R > 0.84).
26646703	7	29	theme	compounds	1295:1303	arg1	characterization					1264:1279	colorimetric characterization	1251:1279	colorimetric characterization of functional compounds used in food processing	1251:1327	The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.
26646703	2	30	theme	native	437:442	arg1	cultivars					452:460	native roselle cultivars	437:460	native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca)	437:502	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	1	31	theme	human	291:295	arg1	health					297:302	human health	291:302	human health	291:302	Roselle is a plant that accumulates anthocyanins significantly, hence its importance as food coloring and as a source of antioxidant compounds for human health.
26646703	0	32	theme	Contrasting	118:128	arg1	Pigmentation					130:141	Contrasting Pigmentation	118:141	Contrasting Pigmentation	118:141	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	7	33	theme	obtained	1182:1189	arg1	tool					1232:1235	an important tool	1219:1235	an important tool to be used in colorimetric characterization of functional compounds used in food processing	1219:1327	The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.
26646703	7	33	theme	obtained	1182:1189	arg1	model					1204:1208	The obtained mathematical model	1178:1208	The obtained mathematical model	1178:1208	The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.
26646703	3	34	dep	used	626:629	arg1	acids					717:721	benzoic, hibiscus and phenolic acids	686:721	benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside)	686:811	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	34	dep	used	626:629	arg1	anthocyanins					743:754	two main anthocyanins	734:754	benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside)	686:811	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	34	dep	used	626:629	arg1	flavonols					675:683	flavonols	675:683	flavonols	675:683	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	34	dep	used	626:629	arg1	flavanols					664:672	flavanols	664:672	flavanols	664:672	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	4	35	theme	antioxidant	818:828	arg1	capacity					830:837	The antioxidant capacity	814:837	The antioxidant capacity	814:837	The antioxidant capacity was evaluated by ABTS and FRAP assays.
26646703	5	36	theme	Tristimulus	878:888	arg1	colorimetry					890:900	Tristimulus colorimetry	878:900	Tristimulus colorimetry	878:900	Tristimulus colorimetry showed to be a useful technique to determine the color-composition relationship, leading to equations that allowed to predict anthocyanin content of roselle (R > 0.84).
26646703	5	36	theme	Tristimulus	878:888	arg1	technique					924:932	a useful technique	915:932	a useful technique to determine the color-composition relationship	915:980	Tristimulus colorimetry showed to be a useful technique to determine the color-composition relationship, leading to equations that allowed to predict anthocyanin content of roselle (R > 0.84).
26646703	2	37	theme	beverages	413:421	arg1	composition					348:358	phenolic composition	339:358	phenolic composition	339:358	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	2	37	theme	beverages	413:421	arg1	capacity					376:383	antioxidant capacity	364:383	antioxidant capacity	364:383	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	0	38	theme	Profile	30:36	arg1	Study					12:16	Comparative Study	0:16	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.	0:142	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	2	39	theme	roselle	444:450	arg1	cultivars					452:460	native roselle cultivars	437:460	native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca)	437:502	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	7	40	theme	functional	1284:1293	arg1	compounds					1295:1303	functional compounds	1284:1303	functional compounds used in food processing	1284:1327	The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.
26646703	0	41	theme	Capacity	51:58	arg1	Study					12:16	Comparative Study	0:16	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.	0:142	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	2	42	theme	different	509:517	arg1	degrees					519:525	different degrees	509:525	different degrees of pigmentation	509:541	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	3	43	theme	phenolic	644:651	arg1	compounds					653:661	phenolic compounds	644:661	phenolic compounds	644:661	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	2	44	theme	phenolic	339:346	arg1	composition					348:358	phenolic composition	339:358	phenolic composition	339:358	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	0	45	theme	Antioxidant	39:49	arg1	Capacity					51:58	Antioxidant Capacity	39:58	Antioxidant Capacity	39:58	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	7	46	theme	food	1313:1316	arg1	processing					1318:1327	food processing	1313:1327	food processing	1313:1327	The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.
26646703	5	47	theme	roselle	1051:1057	arg1	content					1040:1046	anthocyanin content	1028:1046	anthocyanin content of roselle	1028:1057	Tristimulus colorimetry showed to be a useful technique to determine the color-composition relationship, leading to equations that allowed to predict anthocyanin content of roselle (R > 0.84).
26646703	5	48	theme	useful	917:922	arg1	colorimetry					890:900	Tristimulus colorimetry	878:900	Tristimulus colorimetry	878:900	Tristimulus colorimetry showed to be a useful technique to determine the color-composition relationship, leading to equations that allowed to predict anthocyanin content of roselle (R > 0.84).
26646703	5	48	theme	useful	917:922	arg1	technique					924:932	a useful technique	915:932	a useful technique to determine the color-composition relationship	915:980	Tristimulus colorimetry showed to be a useful technique to determine the color-composition relationship, leading to equations that allowed to predict anthocyanin content of roselle (R > 0.84).
26646703	3	49	theme	cyanidin	757:764	arg1	anthocyanins					743:754	two main anthocyanins	734:754	benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside)	686:811	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	49	theme	cyanidin	757:764	arg1	3-sambubioside					766:779	cyanidin 3-sambubioside	757:779	cyanidin 3-sambubioside	757:779	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	50	theme	Chromatographic	597:611	arg1	methods					613:619	Chromatographic methods	597:619	Chromatographic methods	597:619	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	1	51	theme	food	232:235	arg1	coloring					237:244	food coloring	232:244	food coloring	232:244	Roselle is a plant that accumulates anthocyanins significantly, hence its importance as food coloring and as a source of antioxidant compounds for human health.
26646703	0	52	with	Capacity	51:58	arg1	Pigmentation					130:141	Contrasting Pigmentation	118:141	Contrasting Pigmentation	118:141	Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
26646703	6	53	theme	linear	1088:1093	arg1	SLDA					1118:1121	SLDA	1118:1121	SLDA	1118:1121	Also, a stepwise linear discriminant analysis (SLDA) was developed in order to classify roselle cultivars.
26646703	6	53	theme	linear	1088:1093	arg1	analysis					1108:1115	a stepwise linear discriminant analysis	1077:1115	a stepwise linear discriminant analysis (SLDA)	1077:1122	Also, a stepwise linear discriminant analysis (SLDA) was developed in order to classify roselle cultivars.
26646703	6	54	theme	discriminant	1095:1106	arg1	SLDA					1118:1121	SLDA	1118:1121	SLDA	1118:1121	Also, a stepwise linear discriminant analysis (SLDA) was developed in order to classify roselle cultivars.
26646703	6	54	theme	discriminant	1095:1106	arg1	analysis					1108:1115	a stepwise linear discriminant analysis	1077:1115	a stepwise linear discriminant analysis (SLDA)	1077:1122	Also, a stepwise linear discriminant analysis (SLDA) was developed in order to classify roselle cultivars.
26646703	3	55	theme	delphinidin	785:795	arg1	anthocyanins					743:754	two main anthocyanins	734:754	benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside)	686:811	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	55	theme	delphinidin	785:795	arg1	3-sambubioside					797:810	delphinidin 3-sambubioside	785:810	delphinidin 3-sambubioside	785:810	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	56	theme	hibiscus	695:702	arg1	acids					717:721	benzoic, hibiscus and phenolic acids	686:721	benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside)	686:811	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	3	56	theme	hibiscus	695:702	arg1	flavanols					664:672	flavanols	664:672	flavanols	664:672	Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside).
26646703	2	57	theme	antioxidant	364:374	arg1	capacity					376:383	antioxidant capacity	364:383	antioxidant capacity	364:383	This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship.
26646703	7	58	theme	important	1222:1230	arg1	tool					1232:1235	an important tool	1219:1235	an important tool to be used in colorimetric characterization of functional compounds used in food processing	1219:1327	The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.
26646703	7	58	theme	important	1222:1230	arg1	model					1204:1208	The obtained mathematical model	1178:1208	The obtained mathematical model	1178:1208	The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.
24616065	0	0	theme	molecular	87:95	arg1	heparins					104:111	low molecular weight heparins	83:111	low molecular weight heparins	83:111	Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin-III.
24616065	4	1	theme	LWMHs	790:794	arg1	affinities					772:781	the AT-III binding affinities	753:781	the AT-III binding affinities of the LWMHs	753:794	Determination of the AT-III binding affinities of the LWMHs is complicated by their inherent structural heterogeneity and polydispersity.
24616065	5	2	theme	using	981:985	arg1	NMR					990:992	each drug substance using 2D NMR	961:992	each drug substance using 2D NMR	961:992	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues was determined for each drug substance using 2D NMR and used in the interpretation of the ACE results.
24616065	0	3	theme	low	83:85	arg1	heparins					104:111	low molecular weight heparins	83:111	low molecular weight heparins	83:111	Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin-III.
24616065	3	4	dep	heparins	617:624	arg1	enoxaparin					646:655	enoxaparin	646:655	enoxaparin	646:655	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	4	dep	heparins	617:624	arg1	tinzaparin					662:671	tinzaparin	662:671	tinzaparin	662:671	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	4	dep	heparins	617:624	arg1	dalteparin					634:643	dalteparin	634:643	dalteparin	634:643	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	4	dep	heparins	617:624	arg1	heparins					617:624	the low molecular weight heparins	592:624	the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin	592:671	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	4	5	theme	inherent	820:827	arg1	heterogeneity					840:852	their inherent structural heterogeneity	814:852	their inherent structural heterogeneity	814:852	Determination of the AT-III binding affinities of the LWMHs is complicated by their inherent structural heterogeneity and polydispersity.
24616065	5	6	theme	substance	971:979	arg1	NMR					990:992	each drug substance using 2D NMR	961:992	each drug substance using 2D NMR	961:992	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues was determined for each drug substance using 2D NMR and used in the interpretation of the ACE results.
24616065	5	7	used	used	998:1001	arg2	composition					889:899	The fractional composition	874:899	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues	874:940	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues was determined for each drug substance using 2D NMR and used in the interpretation of the ACE results.
24616065	0	8	theme	weight	97:102	arg1	heparins					104:111	low molecular weight heparins	83:111	low molecular weight heparins	83:111	Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin-III.
24616065	5	9	theme	results	1036:1042	arg1	interpretation					1010:1023	the interpretation	1006:1023	the interpretation of the ACE results	1006:1042	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues was determined for each drug substance using 2D NMR and used in the interpretation of the ACE results.
24616065	4	10	theme	AT-III	757:762	arg1	affinities					772:781	the AT-III binding affinities	753:781	the AT-III binding affinities of the LWMHs	753:794	Determination of the AT-III binding affinities of the LWMHs is complicated by their inherent structural heterogeneity and polydispersity.
24616065	1	11	theme	inhibitory	280:289	arg1	activity					291:298	its inhibitory activity	276:298	its inhibitory activity against the coagulation protease factor Xa	276:341	The anticoagulant properties of heparin stem in part from high-affinity binding to antithrombin-III (AT-III) inducing a 300-fold increase in its inhibitory activity against the coagulation protease factor Xa.
24616065	5	12	theme	3-O-sulfo-N-sulfoglucosamine	904:931	arg1	residues					933:940	3-O-sulfo-N-sulfoglucosamine residues	904:940	3-O-sulfo-N-sulfoglucosamine residues	904:940	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues was determined for each drug substance using 2D NMR and used in the interpretation of the ACE results.
24616065	3	13	theme	binding	570:576	arg1	affinities					578:587	the relative AT-III binding affinities	550:587	the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra)	550:733	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	2	14	theme	rare	420:423	arg1	sequence					449:456	the rare heparin pentasaccharide sequence	416:456	the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue	416:511	The minimal structural requirements for AT-III binding are contained in the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue.
24616065	5	15	theme	2D	987:988	arg1	NMR					990:992	each drug substance using 2D NMR	961:992	each drug substance using 2D NMR	961:992	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues was determined for each drug substance using 2D NMR and used in the interpretation of the ACE results.
24616065	0	16	theme	Affinity	0:7	arg1	electrophoresis					19:33	Affinity capillary electrophoresis	0:33	Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin-III	0:132	Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin-III.
24616065	0	17	theme	capillary	9:17	arg1	electrophoresis					19:33	Affinity capillary electrophoresis	0:33	Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin-III	0:132	Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin-III.
24616065	3	18	theme	weight	610:615	arg1	enoxaparin					646:655	enoxaparin	646:655	enoxaparin	646:655	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	18	theme	weight	610:615	arg1	tinzaparin					662:671	tinzaparin	662:671	tinzaparin	662:671	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	18	theme	weight	610:615	arg1	dalteparin					634:643	dalteparin	634:643	dalteparin	634:643	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	18	theme	weight	610:615	arg1	LWMHs					627:631	LWMHs	627:631	LWMHs	627:631	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	18	theme	weight	610:615	arg1	heparins					617:624	the low molecular weight heparins	592:624	the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin	592:671	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	1	19	theme	high-affinity	193:205	arg1	binding					207:213	high-affinity binding	193:213	high-affinity binding to antithrombin-III (AT-III) inducing a 300-fold increase in its inhibitory activity against the coagulation protease factor Xa	193:341	The anticoagulant properties of heparin stem in part from high-affinity binding to antithrombin-III (AT-III) inducing a 300-fold increase in its inhibitory activity against the coagulation protease factor Xa.
24616065	2	20	theme	N-sulfoglucosamine	486:503	arg1	residue					505:511	a 3,6-O-sulfated N-sulfoglucosamine residue	469:511	a 3,6-O-sulfated N-sulfoglucosamine residue	469:511	The minimal structural requirements for AT-III binding are contained in the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue.
24616065	3	21	theme	drug	707:710	arg1	Arixtra					726:732	Arixtra	726:732	Arixtra	726:732	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	21	theme	drug	707:710	arg1	fondaparinux					712:723	the synthetic pentasaccharide drug fondaparinux	677:723	the synthetic pentasaccharide drug fondaparinux (Arixtra)	677:733	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	22	theme	heparins	617:624	arg1	affinities					578:587	the relative AT-III binding affinities	550:587	the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra)	550:733	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	2	23	theme	3,6-O-sulfated	471:484	arg1	residue					505:511	a 3,6-O-sulfated N-sulfoglucosamine residue	469:511	a 3,6-O-sulfated N-sulfoglucosamine residue	469:511	The minimal structural requirements for AT-III binding are contained in the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue.
24616065	3	24	used	used	521:524	arg2	ACE					514:516	ACE	514:516	ACE	514:516	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	5	25	theme	ACE	1032:1034	arg1	results					1036:1042	the ACE results	1028:1042	the ACE results	1028:1042	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues was determined for each drug substance using 2D NMR and used in the interpretation of the ACE results.
24616065	4	26	theme	affinities	772:781	arg1	Determination					736:748	Determination	736:748	Determination of the AT-III binding affinities of the LWMHs	736:794	Determination of the AT-III binding affinities of the LWMHs is complicated by their inherent structural heterogeneity and polydispersity.
24616065	3	27	theme	molecular	600:608	arg1	enoxaparin					646:655	enoxaparin	646:655	enoxaparin	646:655	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	27	theme	molecular	600:608	arg1	tinzaparin					662:671	tinzaparin	662:671	tinzaparin	662:671	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	27	theme	molecular	600:608	arg1	dalteparin					634:643	dalteparin	634:643	dalteparin	634:643	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	27	theme	molecular	600:608	arg1	LWMHs					627:631	LWMHs	627:631	LWMHs	627:631	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	27	theme	molecular	600:608	arg1	heparins					617:624	the low molecular weight heparins	592:624	the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin	592:671	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	5	28	theme	drug	966:969	arg1	NMR					990:992	each drug substance using 2D NMR	961:992	each drug substance using 2D NMR	961:992	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues was determined for each drug substance using 2D NMR and used in the interpretation of the ACE results.
24616065	1	29	theme	coagulation	312:322	arg1	Xa					340:341	the coagulation protease factor Xa	308:341	the coagulation protease factor Xa	308:341	The anticoagulant properties of heparin stem in part from high-affinity binding to antithrombin-III (AT-III) inducing a 300-fold increase in its inhibitory activity against the coagulation protease factor Xa.
24616065	1	30	from	increase	264:271	arg1	activity					291:298	its inhibitory activity	276:298	its inhibitory activity against the coagulation protease factor Xa	276:341	The anticoagulant properties of heparin stem in part from high-affinity binding to antithrombin-III (AT-III) inducing a 300-fold increase in its inhibitory activity against the coagulation protease factor Xa.
24616065	3	31	theme	synthetic	681:689	arg1	Arixtra					726:732	Arixtra	726:732	Arixtra	726:732	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	31	theme	synthetic	681:689	arg1	fondaparinux					712:723	the synthetic pentasaccharide drug fondaparinux	677:723	the synthetic pentasaccharide drug fondaparinux (Arixtra)	677:733	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	2	32	theme	pentasaccharide	433:447	arg1	sequence					449:456	the rare heparin pentasaccharide sequence	416:456	the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue	416:511	The minimal structural requirements for AT-III binding are contained in the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue.
24616065	1	33	theme	protease	324:331	arg1	Xa					340:341	the coagulation protease factor Xa	308:341	the coagulation protease factor Xa	308:341	The anticoagulant properties of heparin stem in part from high-affinity binding to antithrombin-III (AT-III) inducing a 300-fold increase in its inhibitory activity against the coagulation protease factor Xa.
24616065	5	34	theme	residues	933:940	arg1	composition					889:899	The fractional composition	874:899	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues	874:940	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues was determined for each drug substance using 2D NMR and used in the interpretation of the ACE results.
24616065	2	35	theme	heparin	425:431	arg1	sequence					449:456	the rare heparin pentasaccharide sequence	416:456	the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue	416:511	The minimal structural requirements for AT-III binding are contained in the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue.
24616065	0	36	theme	binding	60:66	arg1	affinities					68:77	binding affinities	60:77	binding affinities for low molecular weight heparins and antithrombin-III	60:132	Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin-III.
24616065	1	37	theme	factor	333:338	arg1	Xa					340:341	the coagulation protease factor Xa	308:341	the coagulation protease factor Xa	308:341	The anticoagulant properties of heparin stem in part from high-affinity binding to antithrombin-III (AT-III) inducing a 300-fold increase in its inhibitory activity against the coagulation protease factor Xa.
24616065	3	38	theme	fondaparinux	712:723	arg1	affinities					578:587	the relative AT-III binding affinities	550:587	the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra)	550:733	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	2	39	theme	structural	356:365	arg1	requirements					367:378	The minimal structural requirements	344:378	The minimal structural requirements for AT-III binding	344:397	The minimal structural requirements for AT-III binding are contained in the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue.
24616065	4	40	theme	structural	829:838	arg1	heterogeneity					840:852	their inherent structural heterogeneity	814:852	their inherent structural heterogeneity	814:852	Determination of the AT-III binding affinities of the LWMHs is complicated by their inherent structural heterogeneity and polydispersity.
24616065	1	41	theme	anticoagulant	139:151	arg1	properties					153:162	The anticoagulant properties	135:162	The anticoagulant properties of heparin	135:173	The anticoagulant properties of heparin stem in part from high-affinity binding to antithrombin-III (AT-III) inducing a 300-fold increase in its inhibitory activity against the coagulation protease factor Xa.
24616065	3	42	theme	low	596:598	arg1	enoxaparin					646:655	enoxaparin	646:655	enoxaparin	646:655	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	42	theme	low	596:598	arg1	tinzaparin					662:671	tinzaparin	662:671	tinzaparin	662:671	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	42	theme	low	596:598	arg1	dalteparin					634:643	dalteparin	634:643	dalteparin	634:643	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	42	theme	low	596:598	arg1	LWMHs					627:631	LWMHs	627:631	LWMHs	627:631	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	42	theme	low	596:598	arg1	heparins					617:624	the low molecular weight heparins	592:624	the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin	592:671	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	43	theme	pentasaccharide	691:705	arg1	Arixtra					726:732	Arixtra	726:732	Arixtra	726:732	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	3	43	theme	pentasaccharide	691:705	arg1	fondaparinux					712:723	the synthetic pentasaccharide drug fondaparinux	677:723	the synthetic pentasaccharide drug fondaparinux (Arixtra)	677:733	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	4	44	theme	binding	764:770	arg1	affinities					772:781	the AT-III binding affinities	753:781	the AT-III binding affinities of the LWMHs	753:794	Determination of the AT-III binding affinities of the LWMHs is complicated by their inherent structural heterogeneity and polydispersity.
24616065	3	45	theme	AT-III	563:568	arg1	affinities					578:587	the relative AT-III binding affinities	550:587	the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra)	550:733	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	2	46	theme	minimal	348:354	arg1	requirements					367:378	The minimal structural requirements	344:378	The minimal structural requirements for AT-III binding	344:397	The minimal structural requirements for AT-III binding are contained in the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue.
24616065	2	47	contain	containing	458:467	arg2	residue					505:511	a 3,6-O-sulfated N-sulfoglucosamine residue	469:511	a 3,6-O-sulfated N-sulfoglucosamine residue	469:511	The minimal structural requirements for AT-III binding are contained in the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue.
24616065	2	47	contain	containing	458:467	arg1	sequence					449:456	the rare heparin pentasaccharide sequence	416:456	the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue	416:511	The minimal structural requirements for AT-III binding are contained in the rare heparin pentasaccharide sequence containing a 3,6-O-sulfated N-sulfoglucosamine residue.
24616065	3	48	theme	relative	554:561	arg1	affinities					578:587	the relative AT-III binding affinities	550:587	the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra)	550:733	ACE is used in this work to measure the relative AT-III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
24616065	0	49	theme	affinities	68:77	arg1	determination					43:55	the determination	39:55	the determination of binding affinities for low molecular weight heparins and antithrombin-III	39:132	Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin-III.
24616065	5	50	theme	fractional	878:887	arg1	composition					889:899	The fractional composition	874:899	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues	874:940	The fractional composition of 3-O-sulfo-N-sulfoglucosamine residues was determined for each drug substance using 2D NMR and used in the interpretation of the ACE results.
24616065	1	51	theme	heparin	167:173	arg1	properties					153:162	The anticoagulant properties	135:162	The anticoagulant properties of heparin	135:173	The anticoagulant properties of heparin stem in part from high-affinity binding to antithrombin-III (AT-III) inducing a 300-fold increase in its inhibitory activity against the coagulation protease factor Xa.
24616065	1	52	theme	300-fold	255:262	arg1	increase					264:271	a 300-fold increase	253:271	a 300-fold increase in its inhibitory activity against the coagulation protease factor Xa	253:341	The anticoagulant properties of heparin stem in part from high-affinity binding to antithrombin-III (AT-III) inducing a 300-fold increase in its inhibitory activity against the coagulation protease factor Xa.
24783236	9	0	theme	eudragit	1511:1518	arg1	polymer					1520:1526	eudragit polymer	1511:1526	eudragit polymer	1511:1526	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	11	1	theme	FTIR	1744:1747	arg1	study					1749:1753	FTIR study	1744:1753	FTIR study	1744:1753	FTIR study indicated that no prominent chemical interaction or changes took place between the drug and excipients of prepared formulations.
24783236	6	2	theme	100/Eudragit	1089:1100	arg1	S100					1102:1105	Eudragit RS 100/Eudragit S100	1077:1105	Eudragit RS 100/Eudragit S100	1077:1105	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	4	3	from	effect	640:645	arg1	profiles					786:793	in-vitro release profiles	769:793	in-vitro release profiles (Y1-Y3)	769:801	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	3	from	effect	640:645	arg1	efficiency					820:829	drug entrapment efficiency	804:829	drug entrapment efficiency (Y4)	804:834	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	3	from	effect	640:645	arg1	size					849:852	particle size	840:852	particle size (Y5) of multi-particulates system of indomethacin	840:902	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	3	from	effect	640:645	arg1	Y5					855:856	Y5	855:856	Y5	855:856	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	3	from	effect	640:645	arg1	Y1-Y3					796:800	Y1-Y3	796:800	Y1-Y3	796:800	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	3	from	effect	640:645	arg1	Y4					832:833	Y4	832:833	Y4	832:833	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	4	theme	in-vitro	769:776	arg1	Y1-Y3					796:800	Y1-Y3	796:800	Y1-Y3	796:800	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	4	theme	in-vitro	769:776	arg1	profiles					786:793	in-vitro release profiles	769:793	in-vitro release profiles (Y1-Y3)	769:801	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	6	5	theme	Eudragit	1077:1084	arg1	S100					1102:1105	Eudragit RS 100/Eudragit S100	1077:1105	Eudragit RS 100/Eudragit S100	1077:1105	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	7	6	theme	drug	1293:1296	arg1	release					1298:1304	drug release	1293:1304	drug release	1293:1304	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	12	7	theme	polymers	2009:2016	arg1	matrix					2018:2023	the polymers matrix	2005:2023	the polymers matrix	2005:2023	DSC and XRD studies indicated that drug was present in the amorphous state and may have been homogenously dispersed into the polymers matrix.
24783236	6	8	theme	solvent	1140:1146	arg1	technique					1158:1166	a novel quasi emulsion solvent diffusion technique	1117:1166	a novel quasi emulsion solvent diffusion technique	1117:1166	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	10	9	theme	drug	1652:1655	arg1	data					1665:1668	the drug release data	1648:1668	the drug release data of the selected formulation	1648:1696	It was observed that the drug release data of the selected formulation was similar to the predicted release pattern.
24783236	10	9	theme	drug	1652:1655	arg1	similar					1702:1708	similar	1702:1708	similar	1702:1708	It was observed that the drug release data of the selected formulation was similar to the predicted release pattern.
24783236	7	10	theme	analysis	1280:1287	arg1	basis					1232:1236	the basis	1228:1236	the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies	1228:1321	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	4	11	theme	present	606:612	arg1	work					614:617	the present work	602:617	the present work	602:617	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	5	12	theme	central	973:979	arg1	design					991:996	a three levels face centered central composite design	944:996	a three levels face centered central composite design	944:996	Experiments were designed according to a three levels face centered central composite design.
24783236	4	13	theme	particle	840:847	arg1	Y5					855:856	Y5	855:856	Y5	855:856	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	13	theme	particle	840:847	arg1	size					849:852	particle size	840:852	particle size (Y5) of multi-particulates system of indomethacin	840:902	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	8	14	theme	formulation	1328:1338	arg1	variables					1340:1348	The formulation variables	1324:1348	The formulation variables	1324:1348	The formulation variables were optimized by response surface methodology (RSM).
24783236	6	15	theme	emulsion	1131:1138	arg1	technique					1158:1166	a novel quasi emulsion solvent diffusion technique	1117:1166	a novel quasi emulsion solvent diffusion technique	1117:1166	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	13	16	theme	maximum	2169:2175	arg1	indomethacin					2187:2198	indomethacin	2187:2198	indomethacin	2187:2198	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	13	16	theme	maximum	2169:2175	arg1	amount					2177:2182	maximum amount	2169:2182	maximum amount of indomethacin in intact form	2169:2213	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	4	17	theme	entrapment	809:818	arg1	efficiency					820:829	drug entrapment efficiency	804:829	drug entrapment efficiency (Y4)	804:834	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	17	theme	entrapment	809:818	arg1	Y4					832:833	Y4	832:833	Y4	832:833	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	6	18	theme	novel	1119:1123	arg1	emulsion					1131:1138	a novel quasi emulsion	1117:1138	a novel quasi emulsion solvent diffusion technique	1117:1166	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	12	19	attach	present	1928:1934	arg2	drug					1919:1922	drug	1919:1922	drug	1919:1922	DSC and XRD studies indicated that drug was present in the amorphous state and may have been homogenously dispersed into the polymers matrix.
24783236	12	19	attach	present	1928:1934	arg1	state					1953:1957	the amorphous state	1939:1957	the amorphous state	1939:1957	DSC and XRD studies indicated that drug was present in the amorphous state and may have been homogenously dispersed into the polymers matrix.
24783236	12	20	theme	amorphous	1943:1951	arg1	state					1953:1957	the amorphous state	1939:1957	the amorphous state	1939:1957	DSC and XRD studies indicated that drug was present in the amorphous state and may have been homogenously dispersed into the polymers matrix.
24783236	5	21	theme	three	946:950	arg1	levels					952:957	levels	952:957	levels	952:957	Experiments were designed according to a three levels face centered central composite design.
24783236	9	22	theme	stirring	1611:1618	arg1	speed					1620:1624	stirring speed	1611:1624	stirring speed	1611:1624	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	7	23	theme	particle	1256:1263	arg1	size					1265:1268	particle size	1256:1268	particle size	1256:1268	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	4	24	theme	eudragit	688:695	arg1	X1					706:707	X1	706:707	X1	706:707	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	24	theme	eudragit	688:695	arg1	polymer					697:703	eudragit polymer	688:703	eudragit polymer (X1)	688:708	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	11	25	theme	prepared	1861:1868	arg1	formulations					1870:1881	prepared formulations	1861:1881	prepared formulations	1861:1881	FTIR study indicated that no prominent chemical interaction or changes took place between the drug and excipients of prepared formulations.
24783236	4	26	theme	multi-particulates	862:879	arg1	system					881:886	multi-particulates system	862:886	multi-particulates system of indomethacin	862:902	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	8	27	theme	surface	1377:1383	arg1	RSM					1398:1400	RSM	1398:1400	RSM	1398:1400	The formulation variables were optimized by response surface methodology (RSM).
24783236	8	27	theme	surface	1377:1383	arg1	methodology					1385:1395	response surface methodology	1368:1395	response surface methodology (RSM)	1368:1401	The formulation variables were optimized by response surface methodology (RSM).
24783236	7	28	theme	thermal	1247:1253	arg1	basis					1232:1236	the basis	1228:1236	the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies	1228:1321	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	9	29	dep	decreased	1451:1459	arg1	p<0.05					1476:1481	p<0.05	1476:1481	p<0.05	1476:1481	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	11	30	theme	prominent	1773:1781	arg1	changes					1807:1813	no prominent chemical interaction or changes	1770:1813	no prominent chemical interaction or changes	1770:1813	FTIR study indicated that no prominent chemical interaction or changes took place between the drug and excipients of prepared formulations.
24783236	10	31	theme	formulation	1686:1696	arg1	data					1665:1668	the drug release data	1648:1668	the drug release data of the selected formulation	1648:1696	It was observed that the drug release data of the selected formulation was similar to the predicted release pattern.
24783236	10	31	theme	formulation	1686:1696	arg1	similar					1702:1708	similar	1702:1708	similar	1702:1708	It was observed that the drug release data of the selected formulation was similar to the predicted release pattern.
24783236	0	32	theme	indomethacin	82:93	arg1	system					72:77	system	72:77	system of indomethacin	72:93	Formulation and characterizations of delayed release multi particulates system of indomethacin: optimization by response surface methodology.
24783236	1	33	theme	non-steroidal	172:184	arg1	Indomethacin					142:153	Indomethacin	142:153	Indomethacin	142:153	Indomethacin is a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis.
24783236	1	33	theme	non-steroidal	172:184	arg1	NSAID					210:214	NSAID	210:214	NSAID	210:214	Indomethacin is a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis.
24783236	1	33	theme	non-steroidal	172:184	arg1	drug					204:207	a widely used non-steroidal anti-inflammatory drug	158:207	a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis	158:267	Indomethacin is a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis.
24783236	4	34	theme	surfactant	711:720	arg1	X2					737:738	X2	737:738	X2	737:738	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	34	theme	surfactant	711:720	arg1	concentration					722:734	surfactant concentration	711:734	surfactant concentration (X2)	711:739	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	9	35	from	increase	1570:1577	arg1	concentration					1593:1605	surfactant concentration	1582:1605	surfactant concentration	1582:1605	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	9	35	from	increase	1570:1577	arg1	speed					1620:1624	stirring speed	1611:1624	stirring speed	1611:1624	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	3	36	theme	drug	416:419	arg1	release					421:427	the drug release	412:427	the drug release	412:427	To improve the patient compliance, in this study we aimed to delay the drug release by designing multi-particulates system in the form of microspheres, which would efficiently release the drug into the colon and replace the conventional therapy.
24783236	0	37	theme	response	112:119	arg1	methodology					129:139	response surface methodology	112:139	response surface methodology	112:139	Formulation and characterizations of delayed release multi particulates system of indomethacin: optimization by response surface methodology.
24783236	9	38	from	increase	1489:1496	arg1	amount					1501:1506	amount	1501:1506	amount of eudragit polymer	1501:1526	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	9	38	from	increase	1489:1496	arg1	polymer					1520:1526	eudragit polymer	1511:1526	eudragit polymer	1511:1526	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	13	39	from	form	2210:2213	arg1	delivery					2157:2164	the delivery	2153:2164	the delivery of maximum amount of indomethacin in intact form to the colon	2153:2226	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	9	40	dep	increased	1532:1540	arg1	p<0.05					1557:1562	p<0.05	1557:1562	p<0.05	1557:1562	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	13	41	theme	Eudragit	2091:2098	arg1	microspheres					2105:2116	Eudragit S100 microspheres	2091:2116	Eudragit S100 microspheres	2091:2116	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	3	42	theme	multi-particulates	442:459	arg1	system					461:466	multi-particulates system	442:466	multi-particulates system	442:466	To improve the patient compliance, in this study we aimed to delay the drug release by designing multi-particulates system in the form of microspheres, which would efficiently release the drug into the colon and replace the conventional therapy.
24783236	13	43	theme	microspheres	2105:2116	arg1	combination					2069:2079	the combination	2065:2079	the combination of EC and Eudragit S100 microspheres	2065:2116	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	13	43	theme	microspheres	2105:2116	arg1	useful					2125:2130	useful	2125:2130	useful	2125:2130	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	7	44	theme	kinetics	1306:1313	arg1	studies					1315:1321	kinetics studies	1306:1321	kinetics studies	1306:1321	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	4	45	theme	formulation	650:660	arg1	variables					662:670	formulation variables	650:670	formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3)	650:764	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	10	46	theme	release	1727:1733	arg1	pattern					1735:1741	the predicted release pattern	1713:1741	the predicted release pattern	1713:1741	It was observed that the drug release data of the selected formulation was similar to the predicted release pattern.
24783236	11	47	theme	interaction	1792:1802	arg1	changes					1807:1813	no prominent chemical interaction or changes	1770:1813	no prominent chemical interaction or changes	1770:1813	FTIR study indicated that no prominent chemical interaction or changes took place between the drug and excipients of prepared formulations.
24783236	0	48	theme	delayed	37:43	arg1	multi					53:57	delayed release multi	37:57	delayed release multi	37:57	Formulation and characterizations of delayed release multi particulates system of indomethacin: optimization by response surface methodology.
24783236	0	49	theme	multi	53:57	arg1	characterizations					16:32	characterizations	16:32	characterizations	16:32	Formulation and characterizations of delayed release multi particulates system of indomethacin: optimization by response surface methodology.
24783236	0	49	theme	multi	53:57	arg1	Formulation					0:10	Formulation	0:10	Formulation	0:10	Formulation and characterizations of delayed release multi particulates system of indomethacin: optimization by response surface methodology.
24783236	13	50	theme	EC	2084:2085	arg1	combination					2069:2079	the combination	2065:2079	the combination of EC and Eudragit S100 microspheres	2065:2116	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	13	50	theme	EC	2084:2085	arg1	useful					2125:2130	useful	2125:2130	useful	2125:2130	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	13	51	from	delivery	2157:2164	arg1	form					2210:2213	intact form	2203:2213	intact form	2203:2213	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	2	52	theme	arthritic	325:333	arg1	patients					335:342	arthritic patients	325:342	arthritic patients	325:342	Delayed action (at the morning or night) is needed for arthritic patients.
24783236	4	53	theme	agitation	745:753	arg1	X3					762:763	X3	762:763	X3	762:763	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	53	theme	agitation	745:753	arg1	speed					755:759	agitation speed	745:759	agitation speed (X3)	745:764	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	12	54	theme	XRD	1892:1894	arg1	studies					1896:1902	DSC and XRD studies	1884:1902	DSC and XRD studies	1884:1902	DSC and XRD studies indicated that drug was present in the amorphous state and may have been homogenously dispersed into the polymers matrix.
24783236	3	55	theme	microspheres	483:494	arg1	form					475:478	the form	471:478	the form	471:478	To improve the patient compliance, in this study we aimed to delay the drug release by designing multi-particulates system in the form of microspheres, which would efficiently release the drug into the colon and replace the conventional therapy.
24783236	9	56	theme	polymer	1520:1526	arg1	amount					1501:1506	amount	1501:1506	amount of eudragit polymer	1501:1526	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	9	56	theme	polymer	1520:1526	arg1	polymer					1520:1526	eudragit polymer	1511:1526	eudragit polymer	1511:1526	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	5	57	dep	centered	964:971	arg1	face					959:962	face	959:962	face	959:962	Experiments were designed according to a three levels face centered central composite design.
24783236	5	57	dep	centered	964:971	arg1	levels					952:957	levels	952:957	levels	952:957	Experiments were designed according to a three levels face centered central composite design.
24783236	6	58	theme	RS	1086:1087	arg1	S100					1102:1105	Eudragit RS 100/Eudragit S100	1077:1105	Eudragit RS 100/Eudragit S100	1077:1105	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	13	59	from	useful	2125:2130	arg1	way					2144:2146	a better way	2135:2146	a better way	2135:2146	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	11	60	theme	formulations	1870:1881	arg1	excipients					1847:1856	the drug and excipients	1834:1856	excipients	1847:1856	FTIR study indicated that no prominent chemical interaction or changes took place between the drug and excipients of prepared formulations.
24783236	11	60	theme	formulations	1870:1881	arg1	drug					1838:1841	the drug and excipients	1834:1856	drug	1838:1841	FTIR study indicated that no prominent chemical interaction or changes took place between the drug and excipients of prepared formulations.
24783236	1	61	theme	anti-inflammatory	186:202	arg1	Indomethacin					142:153	Indomethacin	142:153	Indomethacin	142:153	Indomethacin is a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis.
24783236	1	61	theme	anti-inflammatory	186:202	arg1	NSAID					210:214	NSAID	210:214	NSAID	210:214	Indomethacin is a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis.
24783236	1	61	theme	anti-inflammatory	186:202	arg1	drug					204:207	a widely used non-steroidal anti-inflammatory drug	158:207	a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis	158:267	Indomethacin is a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis.
24783236	12	62	theme	DSC	1884:1886	arg1	studies					1896:1902	DSC and XRD studies	1884:1902	DSC and XRD studies	1884:1902	DSC and XRD studies indicated that drug was present in the amorphous state and may have been homogenously dispersed into the polymers matrix.
24783236	13	63	theme	amount	2177:2182	arg1	delivery					2157:2164	the delivery	2153:2164	the delivery of maximum amount of indomethacin in intact form to the colon	2153:2226	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	6	64	theme	diffusion	1148:1156	arg1	technique					1158:1166	a novel quasi emulsion solvent diffusion technique	1117:1166	a novel quasi emulsion solvent diffusion technique	1117:1166	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	10	65	theme	release	1657:1663	arg1	data					1665:1668	the drug release data	1648:1668	the drug release data of the selected formulation	1648:1696	It was observed that the drug release data of the selected formulation was similar to the predicted release pattern.
24783236	10	65	theme	release	1657:1663	arg1	similar					1702:1708	similar	1702:1708	similar	1702:1708	It was observed that the drug release data of the selected formulation was similar to the predicted release pattern.
24783236	13	66	theme	indomethacin	2187:2198	arg1	indomethacin					2187:2198	indomethacin	2187:2198	indomethacin	2187:2198	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	13	66	theme	indomethacin	2187:2198	arg1	amount					2177:2182	maximum amount	2169:2182	maximum amount of indomethacin in intact form	2169:2213	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	4	67	theme	release	778:784	arg1	Y1-Y3					796:800	Y1-Y3	796:800	Y1-Y3	796:800	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	67	theme	release	778:784	arg1	profiles					786:793	in-vitro release profiles	769:793	in-vitro release profiles (Y1-Y3)	769:801	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	7	68	theme	release	1298:1304	arg1	basis					1232:1236	the basis	1228:1236	the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies	1228:1321	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	13	69	from	way	2144:2146	arg1	combination					2069:2079	the combination	2065:2079	the combination of EC and Eudragit S100 microspheres	2065:2116	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	13	69	from	way	2144:2146	arg1	useful					2125:2130	useful	2125:2130	useful	2125:2130	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	6	70	theme	cellulose	1058:1066	arg1	combination					1037:1047	the combination	1033:1047	the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100	1033:1105	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	5	71	theme	composite	981:989	arg1	design					991:996	a three levels face centered central composite design	944:996	a three levels face centered central composite design	944:996	Experiments were designed according to a three levels face centered central composite design.
24783236	3	72	theme	conventional	569:580	arg1	therapy					582:588	the conventional therapy	565:588	the conventional therapy	565:588	To improve the patient compliance, in this study we aimed to delay the drug release by designing multi-particulates system in the form of microspheres, which would efficiently release the drug into the colon and replace the conventional therapy.
24783236	9	73	theme	surfactant	1582:1591	arg1	concentration					1593:1605	surfactant concentration	1582:1605	surfactant concentration	1582:1605	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	6	74	theme	quasi	1125:1129	arg1	emulsion					1131:1138	a novel quasi emulsion	1117:1138	a novel quasi emulsion solvent diffusion technique	1117:1166	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	4	75	theme	work	614:617	arg1	aim					595:597	The aim	591:597	The aim of the present work	591:617	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	12	76	from	state	1953:1957	arg1	present					1928:1934	present	1928:1934	present	1928:1934	DSC and XRD studies indicated that drug was present in the amorphous state and may have been homogenously dispersed into the polymers matrix.
24783236	13	77	theme	better	2137:2142	arg1	way					2144:2146	a better way	2135:2146	a better way	2135:2146	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	4	78	theme	drug	804:807	arg1	efficiency					820:829	drug entrapment efficiency	804:829	drug entrapment efficiency (Y4)	804:834	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	78	theme	drug	804:807	arg1	Y4					832:833	Y4	832:833	Y4	832:833	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	8	79	theme	response	1368:1375	arg1	RSM					1398:1400	RSM	1398:1400	RSM	1398:1400	The formulation variables were optimized by response surface methodology (RSM).
24783236	8	79	theme	response	1368:1375	arg1	methodology					1385:1395	response surface methodology	1368:1395	response surface methodology (RSM)	1368:1401	The formulation variables were optimized by response surface methodology (RSM).
24783236	7	80	theme	SEM	1271:1273	arg1	basis					1232:1236	the basis	1228:1236	the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies	1228:1321	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	7	81	theme	size	1265:1268	arg1	basis					1232:1236	the basis	1228:1236	the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies	1228:1321	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	2	82	theme	Delayed	270:276	arg1	action					278:283	Delayed action	270:283	Delayed action (at the morning or night)	270:309	Delayed action (at the morning or night) is needed for arthritic patients.
24783236	12	83	from	present	1928:1934	arg1	state					1953:1957	the amorphous state	1939:1957	the amorphous state	1939:1957	DSC and XRD studies indicated that drug was present in the amorphous state and may have been homogenously dispersed into the polymers matrix.
24783236	2	84	from	morning	293:299	arg1	action					278:283	Delayed action	270:283	Delayed action (at the morning or night)	270:309	Delayed action (at the morning or night) is needed for arthritic patients.
24783236	6	85	theme	S100	1102:1105	arg1	combination					1037:1047	the combination	1033:1047	the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100	1033:1105	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	1	86	theme	used	167:170	arg1	Indomethacin					142:153	Indomethacin	142:153	Indomethacin	142:153	Indomethacin is a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis.
24783236	1	86	theme	used	167:170	arg1	NSAID					210:214	NSAID	210:214	NSAID	210:214	Indomethacin is a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis.
24783236	1	86	theme	used	167:170	arg1	drug					204:207	a widely used non-steroidal anti-inflammatory drug	158:207	a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis	158:267	Indomethacin is a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis.
24783236	2	87	from	night	304:308	arg1	action					278:283	Delayed action	270:283	Delayed action (at the morning or night)	270:309	Delayed action (at the morning or night) is needed for arthritic patients.
24783236	4	88	theme	system	881:886	arg1	profiles					786:793	in-vitro release profiles	769:793	in-vitro release profiles (Y1-Y3)	769:801	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	88	theme	system	881:886	arg1	efficiency					820:829	drug entrapment efficiency	804:829	drug entrapment efficiency (Y4)	804:834	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	88	theme	system	881:886	arg1	size					849:852	particle size	840:852	particle size (Y5) of multi-particulates system of indomethacin	840:902	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	88	theme	system	881:886	arg1	Y5					855:856	Y5	855:856	Y5	855:856	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	88	theme	system	881:886	arg1	Y1-Y3					796:800	Y1-Y3	796:800	Y1-Y3	796:800	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	88	theme	system	881:886	arg1	Y4					832:833	Y4	832:833	Y4	832:833	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	89	theme	variables	662:670	arg1	effect					640:645	the effect	636:645	the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin	636:902	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	9	90	theme	in-vitro	1422:1429	arg1	Y1-Y3					1440:1444	Y1-Y3	1440:1444	Y1-Y3	1440:1444	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	9	90	theme	in-vitro	1422:1429	arg1	release					1431:1437	in-vitro release	1422:1437	in-vitro release (Y1-Y3)	1422:1445	It was found that in-vitro release (Y1-Y3) was decreased significantly (p<0.05) with increase in amount of eudragit polymer but increased significantly (p<0.05) with increase in surfactant concentration and stirring speed.
24783236	2	91	dep	morning	293:299	arg1	the					289:291	the	289:291	the	289:291	Delayed action (at the morning or night) is needed for arthritic patients.
24783236	3	92	theme	patient	360:366	arg1	compliance					368:377	the patient compliance	356:377	the patient compliance	356:377	To improve the patient compliance, in this study we aimed to delay the drug release by designing multi-particulates system in the form of microspheres, which would efficiently release the drug into the colon and replace the conventional therapy.
24783236	0	93	theme	surface	121:127	arg1	methodology					129:139	response surface methodology	112:139	response surface methodology	112:139	Formulation and characterizations of delayed release multi particulates system of indomethacin: optimization by response surface methodology.
24783236	7	94	theme	FTIR	1241:1244	arg1	basis					1232:1236	the basis	1228:1236	the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies	1228:1321	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	11	95	theme	chemical	1783:1790	arg1	changes					1807:1813	no prominent chemical interaction or changes	1770:1813	no prominent chemical interaction or changes	1770:1813	FTIR study indicated that no prominent chemical interaction or changes took place between the drug and excipients of prepared formulations.
24783236	4	96	theme	polymer	697:703	arg1	amount					678:683	amount	678:683	amount of eudragit polymer (X1)	678:708	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	96	theme	polymer	697:703	arg1	concentration					722:734	surfactant concentration	711:734	surfactant concentration (X2)	711:739	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	96	theme	polymer	697:703	arg1	speed					755:759	agitation speed	745:759	agitation speed (X3)	745:764	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	96	theme	polymer	697:703	arg1	polymer					697:703	eudragit polymer	688:703	eudragit polymer (X1)	688:708	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	96	theme	polymer	697:703	arg1	X3					762:763	X3	762:763	X3	762:763	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	96	theme	polymer	697:703	arg1	X1					706:707	X1	706:707	X1	706:707	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	4	96	theme	polymer	697:703	arg1	X2					737:738	X2	737:738	X2	737:738	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	13	97	theme	S100	2100:2103	arg1	microspheres					2105:2116	Eudragit S100 microspheres	2091:2116	Eudragit S100 microspheres	2091:2116	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	4	98	dep	amount	678:683	arg1	e.g.					672:675	e.g.	672:675	e.g.	672:675	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	10	99	theme	selected	1677:1684	arg1	formulation					1686:1696	the selected formulation	1673:1696	the selected formulation	1673:1696	It was observed that the drug release data of the selected formulation was similar to the predicted release pattern.
24783236	0	100	theme	release	45:51	arg1	multi					53:57	delayed release multi	37:57	delayed release multi	37:57	Formulation and characterizations of delayed release multi particulates system of indomethacin: optimization by response surface methodology.
24783236	4	101	theme	indomethacin	891:902	arg1	system					881:886	multi-particulates system	862:886	multi-particulates system of indomethacin	862:902	The aim of the present work was to elucidate the effect of formulation variables e.g., amount of eudragit polymer (X1), surfactant concentration (X2) and agitation speed (X3) on in-vitro release profiles (Y1-Y3), drug entrapment efficiency (Y4) and particle size (Y5) of multi-particulates system of indomethacin.
24783236	10	102	theme	predicted	1717:1725	arg1	pattern					1735:1741	the predicted release pattern	1713:1741	the predicted release pattern	1713:1741	It was observed that the drug release data of the selected formulation was similar to the predicted release pattern.
24783236	11	103	theme	or	1804:1805	arg1	changes					1807:1813	no prominent chemical interaction or changes	1770:1813	no prominent chemical interaction or changes	1770:1813	FTIR study indicated that no prominent chemical interaction or changes took place between the drug and excipients of prepared formulations.
24783236	7	104	dep	FTIR	1241:1244	arg1	studies					1315:1321	kinetics studies	1306:1321	kinetics studies	1306:1321	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	6	105	theme	ethyl	1052:1056	arg1	EC					1069:1070	EC	1069:1070	EC	1069:1070	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	6	105	theme	ethyl	1052:1056	arg1	cellulose					1058:1066	ethyl cellulose	1052:1066	ethyl cellulose (EC)	1052:1071	Microspheres were formulated with the combination of ethyl cellulose (EC) and Eudragit RS 100/Eudragit S100; by using a novel quasi emulsion solvent diffusion technique.
24783236	12	106	located	present	1928:1934	arg2	drug					1919:1922	drug	1919:1922	drug	1919:1922	DSC and XRD studies indicated that drug was present in the amorphous state and may have been homogenously dispersed into the polymers matrix.
24783236	12	106	located	present	1928:1934	arg1	state					1953:1957	the amorphous state	1939:1957	the amorphous state	1939:1957	DSC and XRD studies indicated that drug was present in the amorphous state and may have been homogenously dispersed into the polymers matrix.
24783236	13	107	theme	intact	2203:2208	arg1	form					2210:2213	intact form	2203:2213	intact form	2203:2213	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	7	108	theme	XRD	1276:1278	arg1	analysis					1280:1287	XRD analysis	1276:1287	XRD analysis	1276:1287	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	7	109	theme	Developed	1169:1177	arg1	formulations					1179:1190	Developed formulations	1169:1190	Developed formulations	1169:1190	Developed formulations were characterized and evaluated on the basis of FTIR, thermal, particle size, SEM, XRD analysis and drug release kinetics studies.
24783236	5	110	dep	design	991:996	arg1	centered					964:971	centered	964:971	centered	964:971	Experiments were designed according to a three levels face centered central composite design.
24783236	13	111	from	amount	2177:2182	arg1	form					2210:2213	intact form	2203:2213	intact form	2203:2213	Therefore this approach suggested that the combination of EC and Eudragit S100 microspheres may be useful in a better way, for the delivery of maximum amount of indomethacin in intact form to the colon.
24783236	1	112	theme	arthritis	259:267	arg1	treatment					246:254	treatment	246:254	treatment of arthritis	246:267	Indomethacin is a widely used non-steroidal anti-inflammatory drug (NSAID) and extensively employed for treatment of arthritis.
28177490	5	0	theme	3-OH	573:576	arg1	12:0					584:587	12:0	584:587	12:0(3-OH)	584:593	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	0	theme	3-OH	573:576	arg1	i/a					579:581	12:0(3-OH) i/a	568:581	12:0(3-OH) i/a	568:581	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	10	1	theme	kinases	1406:1412	arg1	involvement					1357:1367	the involvement	1353:1367	the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling	1353:1485	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	5	2	theme	mannose	414:420	arg1	presence					384:391	the presence	380:391	the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	380:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	3	3	from	Poaceae	270:276	arg1	pathogen					230:237	a Gram-negative pathogen	214:237	a Gram-negative pathogen of crops from the plant family Poaceae	214:276	translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae.
28177490	3	3	from	Poaceae	270:276	arg1	translucens					193:203	translucens	193:203	translucens (Xtt)	193:209	translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae.
28177490	3	3	from	Poaceae	270:276	arg1	crops					242:246	crops	242:246	crops from the plant family Poaceae	242:276	translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae.
28177490	7	4	theme	magnetic	866:873	arg1	spectroscopy					891:902	nuclear magnetic resonance (NMR) spectroscopy	858:902	nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample	858:939	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	5	5	theme	acid	500:503	arg1	presence					384:391	the presence	380:391	the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	380:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	3	6	theme	crops	242:246	arg1	pathogen					230:237	a Gram-negative pathogen	214:237	a Gram-negative pathogen of crops from the plant family Poaceae	214:276	translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae.
28177490	3	6	theme	crops	242:246	arg1	translucens					193:203	translucens	193:203	translucens (Xtt)	193:209	translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae.
28177490	5	7	theme	12:0	568:571	arg1	12:0					584:587	12:0	584:587	12:0(3-OH)	584:593	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	7	theme	12:0	568:571	arg1	i/a					579:581	12:0(3-OH) i/a	568:581	12:0(3-OH) i/a	568:581	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	10	8	theme	induced	1460:1466	arg1	signaling					1477:1485	Xtt LPS induced pathogen signaling	1452:1485	Xtt LPS induced pathogen signaling	1452:1485	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	5	9	dep	13:0	616:619	arg1	a					627:627	a	627:627	a	627:627	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	9	dep	13:0	616:619	arg1	12:0					584:587	12:0	584:587	12:0(3-OH)	584:593	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	9	dep	13:0	616:619	arg1	i/a					579:581	12:0(3-OH) i/a	568:581	12:0(3-OH) i/a	568:581	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	7	10	theme	A	831:831	arg1	backbone					833:840	core-lipid A backbone	820:840	core-lipid A backbone	820:840	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	5	11	theme	14:0	642:645	arg1	acids					521:525	fatty acids	515:525	fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	515:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	11	theme	14:0	642:645	arg1	i/a					653:655	14:0(3-OH) i/a	642:655	14:0(3-OH) i/a	642:655	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	3	12	theme	family	263:268	arg1	Poaceae					270:276	the plant family Poaceae	253:276	the plant family Poaceae	253:276	translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae.
28177490	8	13	theme	phosphodiester	1107:1120	arg1	groups					1122:1127	phosphodiester groups	1107:1127	phosphodiester groups	1107:1127	4-α-Man and Kdo were further substituted via phosphodiester groups by two galactopyranuronic acids.
28177490	5	14	theme	10:0	528:531	arg1	acids					521:525	fatty acids	515:525	fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	515:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	14	theme	10:0	528:531	arg1	i/a					551:553	10:0, 11:0, 11:0(3-OH) i/a	528:553	i/a	551:553	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	15	theme	3-OH	647:650	arg1	acids					521:525	fatty acids	515:525	fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	515:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	15	theme	3-OH	647:650	arg1	i/a					653:655	14:0(3-OH) i/a	642:655	14:0(3-OH) i/a	642:655	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	1	16	dep	translucens	72:82	arg1	translucens					72:82	translucens	72:82	translucens: chemical characterization and determination of signaling events in plant cells.	72:163	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	1	16	dep	translucens	72:82	arg1	characterization					94:109	chemical characterization	85:109	chemical characterization	85:109	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	1	16	dep	translucens	72:82	arg1	determination					115:127	determination	115:127	determination	115:127	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	1	17	theme	signaling	132:140	arg1	events					142:147	signaling events	132:147	signaling events	132:147	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	6	18	theme	rough	684:688	arg1	type					690:693	The rough type	680:693	The rough type of LPS (lipooligosaccharides; LOS)	680:728	The rough type of LPS (lipooligosaccharides; LOS) was isolated and its composition determined utilizing mass spectrometry.
28177490	8	19	theme	galactopyranuronic	1136:1153	arg1	acids					1155:1159	two galactopyranuronic acids	1132:1159	two galactopyranuronic acids	1132:1159	4-α-Man and Kdo were further substituted via phosphodiester groups by two galactopyranuronic acids.
28177490	9	20	theme	stress	1181:1186	arg1	response					1188:1195	a stress response	1179:1195	a stress response	1179:1195	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	7	21	theme	α-D-Manp-	963:971	arg1	2→6					1030:1032	2→6	1030:1032	2→6	1030:1032	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	21	theme	α-D-Manp-	963:971	arg1	-α-Kdo-					1022:1028	α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-	963:1028	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	5	22	theme	acids	521:525	arg1	presence					384:391	the presence	380:391	the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	380:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	23	theme	11:0	556:559	arg1	3-OH					621:624	3-OH	621:624	3-OH	621:624	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	23	theme	11:0	556:559	arg1	13:0					616:619	11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0	556:619	13:0	616:619	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	9	24	theme	cell	1229:1232	arg1	cultures					1234:1241	suspension cell cultures	1218:1241	Nicotiana tabacum suspension cell cultures	1200:1241	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	7	25	theme	-α-Kdo-	1022:1028	arg1	structure					807:815	The structure	803:815	The structure of core-lipid A backbone	803:840	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	25	theme	-α-Kdo-	1022:1028	arg1	-α-D-GlcpN					1050:1059	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	10	26	theme	Pharmacological	1319:1333	arg1	studies					1335:1341	Pharmacological studies	1319:1341	Pharmacological studies	1319:1341	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	5	27	theme	3-OH	545:548	arg1	acids					521:525	fatty acids	515:525	fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	515:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	27	theme	3-OH	545:548	arg1	i/a					551:553	10:0, 11:0, 11:0(3-OH) i/a	528:553	i/a	551:553	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	7	28	theme	1→5	1018:1020	arg1	2→6					1030:1032	2→6	1030:1032	2→6	1030:1032	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	28	theme	1→5	1018:1020	arg1	-α-Kdo-					1022:1028	α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-	963:1028	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	10	29	theme	plasma	1372:1377	arg1	channels					1396:1403	plasma membrane calcium channels	1372:1403	plasma membrane calcium channels	1372:1403	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	7	30	theme	O-deacylated	917:928	arg1	sample					934:939	O-deacylated LOS sample	917:939	O-deacylated LOS sample	917:939	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	9	31	theme	calcium	1263:1269	arg1	signal					1271:1276	a transient calcium signal	1251:1276	namely a transient calcium signal	1244:1276	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	9	31	theme	calcium	1263:1269	arg1	tabacum					1210:1216	Nicotiana tabacum suspension cell cultures	1200:1241	Nicotiana tabacum suspension cell cultures	1200:1241	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	7	32	theme	-α-D-Manp-	1007:1016	arg1	2→6					1030:1032	2→6	1030:1032	2→6	1030:1032	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	32	theme	-α-D-Manp-	1007:1016	arg1	-α-Kdo-					1022:1028	α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-	963:1028	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	0	33	theme	crop	30:33	arg1	Xanthomonas					44:54	the crop pathogen Xanthomonas	26:54	the crop pathogen Xanthomonas	26:54	The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv.
28177490	7	34	theme	1→4	1003:1005	arg1	2→6					1030:1032	2→6	1030:1032	2→6	1030:1032	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	34	theme	1→4	1003:1005	arg1	-α-Kdo-					1022:1028	α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-	963:1028	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	0	35	theme	Xanthomonas	44:54	arg1	lipopolysaccharide					4:21	The lipopolysaccharide	0:21	The lipopolysaccharide of the crop pathogen Xanthomonas	0:54	The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv.
28177490	3	36	from	pathogen	230:237	arg1	Poaceae					270:276	the plant family Poaceae	253:276	the plant family Poaceae	253:276	translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae.
28177490	7	37	theme	-β-D-Glcp-	992:1001	arg1	2→6					1030:1032	2→6	1030:1032	2→6	1030:1032	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	37	theme	-β-D-Glcp-	992:1001	arg1	-α-Kdo-					1022:1028	α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-	963:1028	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	10	38	theme	calcium	1388:1394	arg1	channels					1396:1403	plasma membrane calcium channels	1372:1403	plasma membrane calcium channels	1372:1403	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	1	39	from	determination	115:127	arg1	cells					158:162	plant cells	152:162	plant cells	152:162	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	9	40	theme	H2O2	1300:1303	arg1	generation					1286:1295	the generation	1282:1295	the generation of H2O2	1282:1303	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	4	41	theme	Xtt	311:313	arg1	LPS					303:305	LPS	303:305	LPS	303:305	The lipopolysaccharide (LPS) of Xtt was isolated and chemically characterized.
28177490	4	41	theme	Xtt	311:313	arg1	lipopolysaccharide					283:300	The lipopolysaccharide	279:300	The lipopolysaccharide (LPS) of Xtt	279:313	The lipopolysaccharide (LPS) of Xtt was isolated and chemically characterized.
28177490	7	42	theme	1→3	988:990	arg1	2→6					1030:1032	2→6	1030:1032	2→6	1030:1032	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	42	theme	1→3	988:990	arg1	-α-Kdo-					1022:1028	α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-	963:1028	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	5	43	dep	i/a	551:553	arg1	11:0					540:543	10:0, 11:0, 11:0(3-OH) i/a	528:553	11:0	540:543	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	43	dep	i/a	551:553	arg1	11:0					534:537	10:0, 11:0, 11:0(3-OH) i/a	528:553	11:0	534:537	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	6	44	theme	mass	784:787	arg1	spectrometry					789:800	mass spectrometry	784:800	mass spectrometry	784:800	The rough type of LPS (lipooligosaccharides; LOS) was isolated and its composition determined utilizing mass spectrometry.
28177490	10	45	theme	C	1432:1432	arg1	involvement					1357:1367	the involvement	1353:1367	the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling	1353:1485	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	5	46	theme	acid	445:448	arg1	presence					384:391	the presence	380:391	the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	380:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	7	47	theme	-α-D-Manp-	977:986	arg1	2→6					1030:1032	2→6	1030:1032	2→6	1030:1032	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	47	theme	-α-D-Manp-	977:986	arg1	-α-Kdo-					1022:1028	α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-	963:1028	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	48	theme	resonance	875:883	arg1	spectroscopy					891:902	nuclear magnetic resonance (NMR) spectroscopy	858:902	nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample	858:939	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	49	theme	1→3	973:975	arg1	2→6					1030:1032	2→6	1030:1032	2→6	1030:1032	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	49	theme	1→3	973:975	arg1	-α-Kdo-					1022:1028	α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-	963:1028	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	50	theme	nuclear	858:864	arg1	spectroscopy					891:902	nuclear magnetic resonance (NMR) spectroscopy	858:902	nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample	858:939	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	3	51	theme	Gram-negative	216:228	arg1	pathogen					230:237	a Gram-negative pathogen	214:237	a Gram-negative pathogen of crops from the plant family Poaceae	214:276	translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae.
28177490	3	51	theme	Gram-negative	216:228	arg1	translucens					193:203	translucens	193:203	translucens (Xtt)	193:209	translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae.
28177490	5	52	theme	3-OH	561:564	arg1	3-OH					621:624	3-OH	621:624	3-OH	621:624	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	52	theme	3-OH	561:564	arg1	13:0					616:619	11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0	556:619	13:0	616:619	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	10	53	theme	key	1437:1439	arg1	factors					1441:1447	key factors	1437:1447	key factors in Xtt LPS induced pathogen signaling	1437:1485	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	3	54	theme	plant	257:261	arg1	Poaceae					270:276	the plant family Poaceae	253:276	the plant family Poaceae	253:276	translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae.
28177490	7	55	theme	backbone	833:840	arg1	structure					807:815	The structure	803:815	The structure of core-lipid A backbone	803:840	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	55	theme	backbone	833:840	arg1	-α-D-GlcpN					1050:1059	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	10	56	theme	pathogen	1468:1475	arg1	signaling					1477:1485	Xtt LPS induced pathogen signaling	1452:1485	Xtt LPS induced pathogen signaling	1452:1485	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	5	57	dep	acids	521:525	arg1	13:0					616:619	11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0	556:619	13:0	616:619	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	57	dep	acids	521:525	arg1	3-OH					663:666	3-OH	663:666	3-OH	663:666	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	57	dep	acids	521:525	arg1	13:0					630:633	13:0	630:633	13:0(3-OH)	630:639	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	57	dep	acids	521:525	arg1	3-OH					635:638	3-OH	635:638	3-OH	635:638	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	57	dep	acids	521:525	arg1	i/a					551:553	10:0, 11:0, 11:0(3-OH) i/a	528:553	i/a	551:553	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	57	dep	acids	521:525	arg1	acids					521:525	fatty acids	515:525	fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	515:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	57	dep	acids	521:525	arg1	i/a					653:655	14:0(3-OH) i/a	642:655	14:0(3-OH) i/a	642:655	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	57	dep	acids	521:525	arg1	3-OH					621:624	3-OH	621:624	3-OH	621:624	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	57	dep	acids	521:525	arg1	16:0					673:676	16:0	673:676	16:0	673:676	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	57	dep	acids	521:525	arg1	14:0					658:661	14:0	658:661	14:0(3-OH)	658:667	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	7	58	theme	core-lipid	820:829	arg1	backbone					833:840	core-lipid A backbone	820:840	core-lipid A backbone	820:840	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	5	59	theme	phosphates	451:460	arg1	presence					384:391	the presence	380:391	the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	380:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	60	theme	3-deoxy-D-manno-oct-2-ulopyranosonic	463:498	arg1	Kdo					506:508	Kdo	506:508	Kdo	506:508	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	60	theme	3-deoxy-D-manno-oct-2-ulopyranosonic	463:498	arg1	acid					500:503	3-deoxy-D-manno-oct-2-ulopyranosonic acid	463:503	3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo)	463:509	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	6	61	theme	LPS	698:700	arg1	type					690:693	The rough type	680:693	The rough type of LPS (lipooligosaccharides; LOS)	680:728	The rough type of LPS (lipooligosaccharides; LOS) was isolated and its composition determined utilizing mass spectrometry.
28177490	6	62	dep	lipooligosaccharides	703:722	arg1	LOS					725:727	LOS	725:727	LOS	725:727	The rough type of LPS (lipooligosaccharides; LOS) was isolated and its composition determined utilizing mass spectrometry.
28177490	1	63	theme	events	142:147	arg1	translucens					72:82	translucens	72:82	translucens: chemical characterization and determination of signaling events in plant cells.	72:163	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	1	63	theme	events	142:147	arg1	characterization					94:109	chemical characterization	85:109	chemical characterization	85:109	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	1	63	theme	events	142:147	arg1	determination					115:127	determination	115:127	determination	115:127	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	7	64	theme	-β-D-GlcpN-	1034:1044	arg1	structure					807:815	The structure	803:815	The structure of core-lipid A backbone	803:840	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	7	64	theme	-β-D-GlcpN-	1034:1044	arg1	-α-D-GlcpN					1050:1059	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN	961:1059	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	5	65	theme	fatty	515:519	arg1	acids					521:525	fatty acids	515:525	fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	515:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	65	theme	fatty	515:519	arg1	i/a					653:655	14:0(3-OH) i/a	642:655	14:0(3-OH) i/a	642:655	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	65	theme	fatty	515:519	arg1	i/a					551:553	10:0, 11:0, 11:0(3-OH) i/a	528:553	i/a	551:553	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	1	66	theme	plant	152:156	arg1	cells					158:162	plant cells	152:162	plant cells	152:162	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	10	67	from	factors	1441:1447	arg1	signaling					1477:1485	Xtt LPS induced pathogen signaling	1452:1485	Xtt LPS induced pathogen signaling	1452:1485	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	5	68	theme	rhamnose	396:403	arg1	presence					384:391	the presence	380:391	the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	380:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	9	69	theme	Nicotiana	1200:1208	arg1	signal					1271:1276	a transient calcium signal	1251:1276	namely a transient calcium signal	1244:1276	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	9	69	theme	Nicotiana	1200:1208	arg1	tabacum					1210:1216	Nicotiana tabacum suspension cell cultures	1200:1241	Nicotiana tabacum suspension cell cultures	1200:1241	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	5	70	theme	13:0	602:605	arg1	12:0					584:587	12:0	584:587	12:0(3-OH)	584:593	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	70	theme	13:0	602:605	arg1	i					613:613	13:0(3-OH) i	602:613	13:0(3-OH) i	602:613	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	9	71	theme	suspension	1218:1227	arg1	cultures					1234:1241	suspension cell cultures	1218:1241	Nicotiana tabacum suspension cell cultures	1200:1241	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	5	72	theme	3-OH	607:610	arg1	12:0					584:587	12:0	584:587	12:0(3-OH)	584:593	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	5	72	theme	3-OH	607:610	arg1	i					613:613	13:0(3-OH) i	602:613	13:0(3-OH) i	602:613	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	7	73	theme	LOS	930:932	arg1	sample					934:939	O-deacylated LOS sample	917:939	O-deacylated LOS sample	917:939	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	1	74	theme	chemical	85:92	arg1	translucens					72:82	translucens	72:82	translucens: chemical characterization and determination of signaling events in plant cells.	72:163	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	1	74	theme	chemical	85:92	arg1	characterization					94:109	chemical characterization	85:109	chemical characterization	85:109	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	9	75	dep	tabacum	1210:1216	arg1	cultures					1234:1241	suspension cell cultures	1218:1241	Nicotiana tabacum suspension cell cultures	1200:1241	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	9	76	theme	transient	1253:1261	arg1	signal					1271:1276	a transient calcium signal	1251:1276	namely a transient calcium signal	1244:1276	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	9	76	theme	transient	1253:1261	arg1	tabacum					1210:1216	Nicotiana tabacum suspension cell cultures	1200:1241	Nicotiana tabacum suspension cell cultures	1200:1241	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	0	77	theme	pathogen	35:42	arg1	Xanthomonas					44:54	the crop pathogen Xanthomonas	26:54	the crop pathogen Xanthomonas	26:54	The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv.
28177490	1	78	from	characterization	94:109	arg1	cells					158:162	plant cells	152:162	plant cells	152:162	translucens: chemical characterization and determination of signaling events in plant cells.
28177490	7	79	theme	NMR	886:888	arg1	spectroscopy					891:902	nuclear magnetic resonance (NMR) spectroscopy	858:902	nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample	858:939	The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be: α-D-Manp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-α-D-Manp-(1→5)-α-Kdo-(2→6)-β-D-GlcpN-(1→6)-α-D-GlcpN.
28177490	10	80	theme	channels	1396:1403	arg1	involvement					1357:1367	the involvement	1353:1367	the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling	1353:1485	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	5	81	theme	glucose	423:429	arg1	presence					384:391	the presence	380:391	the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	380:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	10	82	theme	membrane	1379:1386	arg1	channels					1396:1403	plasma membrane calcium channels	1372:1403	plasma membrane calcium channels	1372:1403	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	5	83	theme	galacturonic	432:443	arg1	acid					445:448	galacturonic acid	432:448	galacturonic acid	432:448	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
28177490	9	84	theme	Xtt	1162:1164	arg1	LPS					1166:1168	Xtt LPS	1162:1168	Xtt LPS	1162:1168	Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed.
28177490	10	85	theme	phospholipase	1418:1430	arg1	C					1432:1432	phospholipase C	1418:1432	phospholipase C	1418:1432	Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
28177490	5	86	theme	xylose	406:411	arg1	presence					384:391	the presence	380:391	the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)	380:677	The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0).
29186191	5	0	theme	O	811:811	arg1	antigen					813:819	lipopolysaccharide O antigen	792:819	lipopolysaccharide O antigen	792:819	The presence of lipopolysaccharide O antigen enables vesicles to bypass clathrin-mediated endocytosis, which enhances both their entry rate and efficiency into host cells.
29186191	4	1	theme	uptake	681:686	arg1	efficiency					717:726	efficiency	717:726	efficiency	717:726	We found that the route of vesicular uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition.
29186191	4	1	theme	uptake	681:686	arg1	route					662:666	the route	658:666	the route of vesicular uptake	658:686	We found that the route of vesicular uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition.
29186191	4	1	theme	uptake	681:686	arg1	kinetics					704:711	entry kinetics	698:711	entry kinetics	698:711	We found that the route of vesicular uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition.
29186191	0	2	theme	vesicles	84:91	arg1	kinetics					47:54	the entry kinetics	37:54	the entry kinetics of bacterial outer membrane vesicles into host cells	37:107	Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells.
29186191	6	3	theme	major	1033:1037	arg1	composition					989:999	the composition	985:999	the composition of the bacterial cell wall	985:1026	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
29186191	6	3	theme	major	1033:1037	arg1	determinant					1039:1049	a major determinant	1031:1049	a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections	1031:1136	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
29186191	4	4	theme	vesicular	671:679	arg1	uptake					681:686	vesicular uptake	671:686	vesicular uptake	671:686	We found that the route of vesicular uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition.
29186191	1	5	theme	disease	265:271	arg1	pathogenesis					273:284	disease pathogenesis	265:284	disease pathogenesis	265:284	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	4	6	theme	cell	753:756	arg1	composition					763:773	bacterial cell wall composition	743:773	bacterial cell wall composition	743:773	We found that the route of vesicular uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition.
29186191	2	7	theme	current	300:306	arg1	understanding					320:332	our current mechanistic understanding	296:332	our current mechanistic understanding of vesicle-host cell interactions	296:366	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	2	8	theme	mechanistic	308:318	arg1	understanding					320:332	our current mechanistic understanding	296:332	our current mechanistic understanding of vesicle-host cell interactions	296:366	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	0	9	theme	host	98:101	arg1	cells					103:107	host cells	98:107	host cells	98:107	Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells.
29186191	6	10	theme	virulence	1088:1096	arg1	factors					1098:1104	virulence factors	1088:1104	virulence factors	1088:1104	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
29186191	1	11	theme	nano-sized	138:147	arg1	vesicles					125:132	Outer membrane vesicles	110:132	Outer membrane vesicles	110:132	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	1	11	theme	nano-sized	138:147	arg1	microvesicles					149:161	nano-sized microvesicles	138:161	nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria	138:216	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	6	12	theme	delivery	1076:1083	arg1	composition					989:999	the composition	985:999	the composition of the bacterial cell wall	985:1026	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
29186191	6	12	theme	delivery	1076:1083	arg1	determinant					1039:1049	a major determinant	1031:1049	a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections	1031:1136	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
29186191	4	13	theme	bacterial	743:751	arg1	composition					763:773	bacterial cell wall composition	743:773	bacterial cell wall composition	743:773	We found that the route of vesicular uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition.
29186191	1	14	theme	outer	177:181	arg1	membrane					183:190	the outer membrane	173:190	the outer membrane of Gram-negative bacteria	173:216	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	3	15	from	real-time	581:589	arg1	kinetics					536:543	the kinetics	532:543	the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe	532:641	Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe.
29186191	2	16	theme	cargo	449:453	arg1	delivery					455:462	cargo delivery	449:462	cargo delivery to host cells	449:476	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	6	17	theme	secretion-independent	1054:1074	arg1	delivery					1076:1083	secretion-independent delivery	1054:1083	secretion-independent delivery of virulence factors during Gram-negative infections	1054:1136	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
29186191	2	18	theme	vesicle-host	337:348	arg1	interactions					355:366	vesicle-host cell interactions	337:366	vesicle-host cell interactions	337:366	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	5	19	theme	clathrin-mediated	848:864	arg1	endocytosis					866:876	clathrin-mediated endocytosis	848:876	clathrin-mediated endocytosis	848:876	The presence of lipopolysaccharide O antigen enables vesicles to bypass clathrin-mediated endocytosis, which enhances both their entry rate and efficiency into host cells.
29186191	0	20	theme	Lipopolysaccharide	0:17	arg1	structure					19:27	Lipopolysaccharide structure	0:27	Lipopolysaccharide structure	0:27	Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells.
29186191	3	21	theme	vesicle	548:554	arg1	entry					556:560	vesicle entry	548:560	vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe	548:641	Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe.
29186191	2	22	theme	methods	392:398	arg1	lack					384:387	a lack	382:387	a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells	382:476	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	3	23	from	kinetics	536:543	arg1	real-time					581:589	real-time	581:589	real-time	581:589	Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe.
29186191	5	24	theme	host	936:939	arg1	cells					941:945	host cells	936:945	host cells	936:945	The presence of lipopolysaccharide O antigen enables vesicles to bypass clathrin-mediated endocytosis, which enhances both their entry rate and efficiency into host cells.
29186191	2	25	theme	vesicle	431:437	arg1	entry					439:443	vesicle entry	431:443	vesicle entry	431:443	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	2	26	theme	entry	439:443	arg1	kinetics					419:426	the rapid kinetics	409:426	the rapid kinetics of vesicle entry	409:443	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	2	26	theme	entry	439:443	arg1	delivery					455:462	cargo delivery	449:462	cargo delivery to host cells	449:476	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	1	27	theme	Outer	110:114	arg1	vesicles					125:132	Outer membrane vesicles	110:132	Outer membrane vesicles	110:132	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	1	27	theme	Outer	110:114	arg1	microvesicles					149:161	nano-sized microvesicles	138:161	nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria	138:216	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	5	28	theme	antigen	813:819	arg1	presence					780:787	The presence	776:787	The presence of lipopolysaccharide O antigen	776:819	The presence of lipopolysaccharide O antigen enables vesicles to bypass clathrin-mediated endocytosis, which enhances both their entry rate and efficiency into host cells.
29186191	1	29	theme	Gram-negative	195:207	arg1	bacteria					209:216	Gram-negative bacteria	195:216	Gram-negative bacteria	195:216	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	0	30	theme	entry	41:45	arg1	kinetics					47:54	the entry kinetics	37:54	the entry kinetics of bacterial outer membrane vesicles into host cells	37:107	Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells.
29186191	1	31	theme	important	227:235	arg1	roles					237:241	important roles	227:241	important roles	227:241	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	2	32	theme	host	467:470	arg1	cells					472:476	host cells	467:476	host cells	467:476	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	6	33	theme	wall	1023:1026	arg1	composition					989:999	the composition	985:999	the composition of the bacterial cell wall	985:1026	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
29186191	6	33	theme	wall	1023:1026	arg1	determinant					1039:1049	a major determinant	1031:1049	a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections	1031:1136	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
29186191	0	34	theme	outer	69:73	arg1	vesicles					84:91	bacterial outer membrane vesicles	59:91	bacterial outer membrane vesicles	59:91	Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells.
29186191	3	35	theme	host	567:570	arg1	cells					572:576	host cells	567:576	host cells	567:576	Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe.
29186191	1	36	theme	membrane	116:123	arg1	vesicles					125:132	Outer membrane vesicles	110:132	Outer membrane vesicles	110:132	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	1	36	theme	membrane	116:123	arg1	microvesicles					149:161	nano-sized microvesicles	138:161	nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria	138:216	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	5	37	theme	entry	905:909	arg1	rate					911:914	their entry rate	899:914	their entry rate	899:914	The presence of lipopolysaccharide O antigen enables vesicles to bypass clathrin-mediated endocytosis, which enhances both their entry rate and efficiency into host cells.
29186191	3	38	theme	entry	556:560	arg1	kinetics					536:543	the kinetics	532:543	the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe	532:641	Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe.
29186191	4	39	theme	wall	758:761	arg1	composition					763:773	bacterial cell wall composition	743:773	bacterial cell wall composition	743:773	We found that the route of vesicular uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition.
29186191	6	40	theme	bacterial	1008:1016	arg1	wall					1023:1026	the bacterial cell wall	1004:1026	the bacterial cell wall	1004:1026	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
29186191	2	41	theme	interactions	355:366	arg1	understanding					320:332	our current mechanistic understanding	296:332	our current mechanistic understanding of vesicle-host cell interactions	296:366	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	6	42	theme	Gram-negative	1113:1125	arg1	infections					1127:1136	Gram-negative infections	1113:1136	Gram-negative infections	1113:1136	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
29186191	6	43	theme	cell	1018:1021	arg1	wall					1023:1026	the bacterial cell wall	1004:1026	the bacterial cell wall	1004:1026	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
29186191	3	44	dep	encoded	611:617	arg1	vesicle-targeted					620:635	vesicle-targeted	620:635	vesicle-targeted	620:635	Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe.
29186191	3	45	theme	encoded	611:617	arg1	probe					637:641	a genetically encoded, vesicle-targeted probe	597:641	a genetically encoded, vesicle-targeted probe	597:641	Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe.
29186191	2	46	theme	cell	350:353	arg1	interactions					355:366	vesicle-host cell interactions	337:366	vesicle-host cell interactions	337:366	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	3	47	from	entry	556:560	arg1	real-time					581:589	real-time	581:589	real-time	581:589	Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe.
29186191	4	48	theme	entry	698:702	arg1	kinetics					704:711	entry kinetics	698:711	entry kinetics	698:711	We found that the route of vesicular uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition.
29186191	0	49	theme	membrane	75:82	arg1	vesicles					84:91	bacterial outer membrane vesicles	59:91	bacterial outer membrane vesicles	59:91	Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells.
29186191	2	50	theme	rapid	413:417	arg1	kinetics					419:426	the rapid kinetics	409:426	the rapid kinetics of vesicle entry	409:443	However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells.
29186191	1	51	theme	bacteria	209:216	arg1	membrane					183:190	the outer membrane	173:190	the outer membrane of Gram-negative bacteria	173:216	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	3	52	theme	sensitive	506:514	arg1	method					516:521	a highly sensitive method	497:521	a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe	497:641	Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe.
29186191	1	53	theme	immune	246:251	arg1	priming					253:259	immune priming	246:259	immune priming	246:259	Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis.
29186191	0	54	theme	bacterial	59:67	arg1	vesicles					84:91	bacterial outer membrane vesicles	59:91	bacterial outer membrane vesicles	59:91	Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells.
29186191	5	55	theme	lipopolysaccharide	792:809	arg1	antigen					813:819	lipopolysaccharide O antigen	792:819	lipopolysaccharide O antigen	792:819	The presence of lipopolysaccharide O antigen enables vesicles to bypass clathrin-mediated endocytosis, which enhances both their entry rate and efficiency into host cells.
29186191	6	56	theme	factors	1098:1104	arg1	delivery					1076:1083	secretion-independent delivery	1054:1083	secretion-independent delivery of virulence factors during Gram-negative infections	1054:1136	Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
25362136	5	0	theme	fluid	1021:1025	arg1	phase					1027:1031	the extracellular fluid phase	1003:1031	the extracellular fluid phase	1003:1031	Albumin was excluded from 55±4% (n=8) of the extracellular fluid phase.
25362136	7	1	from	increase	1462:1469	arg1	hyaluronan					1474:1483	hyaluronan	1474:1483	hyaluronan	1474:1483	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	7	1	from	increase	1462:1469	arg1	hydration					1422:1430	skin hydration	1417:1430	skin hydration	1417:1430	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	7	1	from	increase	1462:1469	arg1	GAGs					1445:1448	sulfated GAGs	1436:1448	sulfated GAGs	1436:1448	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	2	2	theme	exclusion	325:333	arg1	phenomenon					335:344	This exclusion phenomenon	320:344	This exclusion phenomenon	320:344	This exclusion phenomenon is of importance for transcapillary fluid and solute exchange.
25362136	0	3	from	changes	12:18	arg1	composition					55:65	rat dermal extracellular matrix composition	23:65	rat dermal extracellular matrix composition	23:65	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	4	4	theme	mass	787:790	arg1	determination					744:756	the exact determination	734:756	the exact determination of albumin concentration and mass	734:790	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	8	5	theme	delayed	1644:1650	arg1	adjustments					1652:1662	delayed adjustments	1644:1662	delayed adjustments of fluid perturbations	1644:1685	Our findings suggest that the changes in the ECM in aged skin may result in delayed adjustments of fluid perturbations and reduced ability for salt storage.
25362136	3	6	theme	exclusion	475:483	arg1	range					453:457	the range	449:457	the range of interstitial exclusion in rat skin	449:495	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	6	7	theme	comparable	1325:1334	arg1	size					1336:1339	comparable size	1325:1339	comparable size	1325:1339	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	6	7	theme	comparable	1325:1334	arg1	VEF=-0.036·pI+0.719					1342:1360	VEF=-0.036·pI+0.719	1342:1360	VEF=-0.036·pI+0.719 (P=0.04)	1342:1369	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	7	8	theme	sulfated	1436:1443	arg1	GAGs					1445:1448	sulfated GAGs	1436:1448	sulfated GAGs	1436:1448	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	0	9	theme	proteins	101:108	arg1	distribution					78:89	the distribution	74:89	the distribution of plasma proteins	74:108	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	0	9	theme	proteins	101:108	arg1	function					115:122	a function	113:122	a function of size and charge	113:141	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	3	10	from	exclusion	475:483	arg1	skin					492:495	rat skin	488:495	rat skin	488:495	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	7	11	theme	skin	1417:1420	arg1	hydration					1422:1430	skin hydration	1417:1430	skin hydration	1417:1430	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	0	12	theme	plasma	94:99	arg1	proteins					101:108	plasma proteins	94:108	plasma proteins	94:108	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	8	13	theme	salt	1711:1714	arg1	storage					1716:1722	salt storage	1711:1722	salt storage	1711:1722	Our findings suggest that the changes in the ECM in aged skin may result in delayed adjustments of fluid perturbations and reduced ability for salt storage.
25362136	6	14	with	proteins	1311:1318	arg1	size					1336:1339	comparable size	1325:1339	comparable size	1325:1339	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	6	14	with	proteins	1311:1318	arg1	VEF=-0.036·pI+0.719					1342:1360	VEF=-0.036·pI+0.719	1342:1360	VEF=-0.036·pI+0.719 (P=0.04)	1342:1369	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	3	15	theme	interstitial	462:473	arg1	exclusion					475:483	interstitial exclusion	462:483	interstitial exclusion in rat skin	462:495	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	7	16	theme	other	1539:1543	arg1	probes					1560:1565	the other macromolecular probes	1535:1565	the other macromolecular probes	1535:1565	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	4	17	theme	relative	877:884	arg1	numbers					869:875	the corresponding numbers	851:875	the corresponding numbers relative to albumin	851:895	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	1	18	from	fraction	273:280	arg1	macromolecules					251:264	macromolecules	251:264	macromolecules from a fraction of the interstitial fluid (IF) phase	251:317	Collagen and glycosaminoglycans (GAGs) constituting the ECM may limit the space available and thus exclude macromolecules from a fraction of the interstitial fluid (IF) phase.
25362136	6	19	dep	significant	1053:1063	arg1	positive					1066:1073	positive	1066:1073	positive	1066:1073	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	7	20	theme	macromolecular	1545:1558	arg1	probes					1560:1565	the other macromolecular probes	1535:1565	the other macromolecular probes	1535:1565	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	2	21	theme	transcapillary	367:380	arg1	exchange					399:406	transcapillary fluid and solute exchange	367:406	transcapillary fluid and solute exchange	367:406	This exclusion phenomenon is of importance for transcapillary fluid and solute exchange.
25362136	3	22	from	change	592:597	arg1	composition					615:625	interstitial composition	602:625	interstitial composition	602:625	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	8	23	theme	perturbations	1673:1685	arg1	adjustments					1652:1662	delayed adjustments	1644:1662	delayed adjustments of fluid perturbations	1644:1685	Our findings suggest that the changes in the ECM in aged skin may result in delayed adjustments of fluid perturbations and reduced ability for salt storage.
25362136	8	23	theme	perturbations	1673:1685	arg1	ability					1699:1705	reduced ability	1691:1705	reduced ability for salt storage	1691:1722	Our findings suggest that the changes in the ECM in aged skin may result in delayed adjustments of fluid perturbations and reduced ability for salt storage.
25362136	0	24	theme	Age-related	0:10	arg1	changes					12:18	Age-related changes	0:18	Age-related changes in rat dermal extracellular matrix composition	0:65	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	7	25	from	reduction	1404:1412	arg1	hyaluronan					1474:1483	hyaluronan	1474:1483	hyaluronan	1474:1483	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	7	25	from	reduction	1404:1412	arg1	hydration					1422:1430	skin hydration	1417:1430	skin hydration	1417:1430	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	7	25	from	reduction	1404:1412	arg1	GAGs					1445:1448	sulfated GAGs	1436:1448	sulfated GAGs	1436:1448	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	3	26	theme	aging	658:662	arg1	consequence					643:653	a consequence	641:653	a consequence of aging	641:662	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	6	27	theme	excluded	1144:1151	arg1	volume					1153:1158	fractional excluded volume	1133:1158	fractional excluded volume (VEF)	1133:1164	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	6	27	theme	excluded	1144:1151	arg1	VEF					1161:1163	VEF	1161:1163	VEF	1161:1163	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	8	28	theme	fluid	1667:1671	arg1	perturbations					1673:1685	fluid perturbations	1667:1685	fluid perturbations	1667:1685	Our findings suggest that the changes in the ECM in aged skin may result in delayed adjustments of fluid perturbations and reduced ability for salt storage.
25362136	4	29	theme	corresponding	855:867	arg1	numbers					869:875	the corresponding numbers	851:875	the corresponding numbers relative to albumin	851:895	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	4	30	theme	tissue	802:807	arg1	eluate					809:814	tissue eluate	802:814	tissue eluate by HPLC	802:822	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	1	31	theme	phase	313:317	arg1	fraction					273:280	a fraction	271:280	a fraction of the interstitial fluid (IF) phase	271:317	Collagen and glycosaminoglycans (GAGs) constituting the ECM may limit the space available and thus exclude macromolecules from a fraction of the interstitial fluid (IF) phase.
25362136	7	32	theme	corresponding	1492:1504	arg1	VEF					1515:1517	a corresponding, reduced VEF	1490:1517	VEF	1515:1517	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	3	33	theme	interstitial	602:613	arg1	composition					615:625	interstitial composition	602:625	interstitial composition	602:625	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	3	34	from	range	453:457	arg1	skin					492:495	rat skin	488:495	rat skin	488:495	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	4	35	theme	albumin	761:767	arg1	concentration					769:781	albumin concentration	761:781	albumin concentration	761:781	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	0	36	theme	rat	23:25	arg1	composition					55:65	rat dermal extracellular matrix composition	23:65	rat dermal extracellular matrix composition	23:65	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	6	37	theme	probe	1095:1099	arg1	Stokes-Einstein					1101:1115	probe Stokes-Einstein	1095:1115	probe Stokes-Einstein (SE) radius	1095:1127	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	6	37	theme	probe	1095:1099	arg1	SE					1118:1119	SE	1118:1119	SE	1118:1119	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	4	38	theme	exact	738:742	arg1	determination					744:756	the exact determination	734:756	the exact determination of albumin concentration and mass	734:790	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	8	39	theme	reduced	1691:1697	arg1	ability					1699:1705	reduced ability	1691:1705	reduced ability for salt storage	1691:1722	Our findings suggest that the changes in the ECM in aged skin may result in delayed adjustments of fluid perturbations and reduced ability for salt storage.
25362136	4	40	theme	quantitative	937:948	arg1	proteomics					950:959	quantitative proteomics	937:959	quantitative proteomics	937:959	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	7	41	theme	reduced	1507:1513	arg1	VEF					1515:1517	a corresponding, reduced VEF	1490:1517	VEF	1515:1517	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	2	42	theme	solute	392:397	arg1	exchange					399:406	transcapillary fluid and solute exchange	367:406	transcapillary fluid and solute exchange	367:406	This exclusion phenomenon is of importance for transcapillary fluid and solute exchange.
25362136	0	43	theme	extracellular	34:46	arg1	composition					55:65	rat dermal extracellular matrix composition	23:65	rat dermal extracellular matrix composition	23:65	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	0	44	theme	dermal	27:32	arg1	composition					55:65	rat dermal extracellular matrix composition	23:65	rat dermal extracellular matrix composition	23:65	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	4	45	theme	probe	910:914	arg1	proteins					916:923	probe proteins	910:923	probe proteins assessed by quantitative proteomics	910:959	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	0	46	theme	size	127:130	arg1	distribution					78:89	the distribution	74:89	the distribution of plasma proteins	74:108	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	0	46	theme	size	127:130	arg1	function					115:122	a function	113:122	a function of size and charge	113:141	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	3	47	theme	electrical	552:561	arg1	charge					563:568	electrical charge	552:568	electrical charge	552:568	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	2	48	theme	fluid	382:386	arg1	exchange					399:406	transcapillary fluid and solute exchange	367:406	transcapillary fluid and solute exchange	367:406	This exclusion phenomenon is of importance for transcapillary fluid and solute exchange.
25362136	4	49	theme	proteins	916:923	arg1	set					903:905	a set	901:905	a set of probe proteins assessed by quantitative proteomics	901:959	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	4	49	theme	proteins	916:923	arg1	proteins					916:923	probe proteins	910:923	probe proteins assessed by quantitative proteomics	910:959	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	1	50	theme	interstitial	289:300	arg1	fluid					302:306	interstitial fluid	289:306	the interstitial fluid (IF) phase	285:317	Collagen and glycosaminoglycans (GAGs) constituting the ECM may limit the space available and thus exclude macromolecules from a fraction of the interstitial fluid (IF) phase.
25362136	6	51	theme	negative	1235:1242	arg1	correlation					1244:1254	a negative correlation	1233:1254	a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04)	1233:1369	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	7	52	theme	significant	1392:1402	arg1	reduction					1404:1412	a significant reduction	1390:1412	a significant reduction in skin hydration and sulfated GAGs	1390:1448	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	3	53	theme	molecular	530:538	arg1	weights					540:546	molecular weights	530:546	molecular weights	530:546	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	4	54	theme	concentration	769:781	arg1	determination					744:756	the exact determination	734:756	the exact determination of albumin concentration and mass	734:790	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	5	55	theme	phase	1027:1031	arg1	phase					1027:1031	the extracellular fluid phase	1003:1031	the extracellular fluid phase	1003:1031	Albumin was excluded from 55±4% (n=8) of the extracellular fluid phase.
25362136	5	55	theme	phase	1027:1031	arg1	n=8					995:997	n=8	995:997	n=8	995:997	Albumin was excluded from 55±4% (n=8) of the extracellular fluid phase.
25362136	5	55	theme	phase	1027:1031	arg1	%					992:992	55±4%	988:992	55±4% (n=8) of the extracellular fluid phase	988:1031	Albumin was excluded from 55±4% (n=8) of the extracellular fluid phase.
25362136	0	56	theme	matrix	48:53	arg1	composition					55:65	rat dermal extracellular matrix composition	23:65	rat dermal extracellular matrix composition	23:65	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	1	57	theme	fluid	302:306	arg1	phase					313:317	the interstitial fluid (IF) phase	285:317	the interstitial fluid (IF) phase	285:317	Collagen and glycosaminoglycans (GAGs) constituting the ECM may limit the space available and thus exclude macromolecules from a fraction of the interstitial fluid (IF) phase.
25362136	0	58	theme	charge	136:141	arg1	distribution					78:89	the distribution	74:89	the distribution of plasma proteins	74:108	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	0	58	theme	charge	136:141	arg1	function					115:122	a function	113:122	a function of size and charge	113:141	Age-related changes in rat dermal extracellular matrix composition affect the distribution of plasma proteins as a function of size and charge.
25362136	3	59	theme	weights	540:546	arg1	span					522:525	a span	520:525	a span of molecular weights and electrical charge	520:568	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	6	60	theme	VEF=0.078·SE	1180:1191	arg1	radius+0.269					1193:1204	VEF=0.078·SE radius+0.269	1180:1204	VEF=0.078·SE radius+0.269 (P<0.001)	1180:1214	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	6	60	theme	VEF=0.078·SE	1180:1191	arg1	P<0.001					1207:1213	P<0.001	1207:1213	P<0.001	1207:1213	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	8	61	from	changes	1598:1604	arg1	ECM					1613:1615	the ECM	1609:1615	the ECM	1609:1615	Our findings suggest that the changes in the ECM in aged skin may result in delayed adjustments of fluid perturbations and reduced ability for salt storage.
25362136	8	61	from	changes	1598:1604	arg1	skin					1625:1628	aged skin	1620:1628	aged skin	1620:1628	Our findings suggest that the changes in the ECM in aged skin may result in delayed adjustments of fluid perturbations and reduced ability for salt storage.
25362136	4	62	theme	novel	708:712	arg1	approach					714:721	a novel approach	706:721	a novel approach	706:721	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	7	63	from	VEF	1515:1517	arg1	hyaluronan					1474:1483	hyaluronan	1474:1483	hyaluronan	1474:1483	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	7	63	from	VEF	1515:1517	arg1	hydration					1422:1430	skin hydration	1417:1430	skin hydration	1417:1430	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	7	63	from	VEF	1515:1517	arg1	GAGs					1445:1448	sulfated GAGs	1436:1448	sulfated GAGs	1436:1448	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	4	64	used	used	701:704	arg2	we					698:699	we	698:699	we	698:699	To this end, we used a novel approach, involving the exact determination of albumin concentration and mass in IF and tissue eluate by HPLC and thereafter, expressing the corresponding numbers relative to albumin for a set of probe proteins assessed by quantitative proteomics.
25362136	6	65	theme	significant	1053:1063	arg1	correlation					1075:1085	a highly significant, positive correlation	1044:1085	a highly significant, positive correlation	1044:1085	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	6	66	theme	Stokes-Einstein	1101:1115	arg1	radius					1122:1127	probe Stokes-Einstein (SE) radius	1095:1127	probe Stokes-Einstein (SE) radius	1095:1127	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	7	67	theme	moderate	1453:1460	arg1	increase					1462:1469	a moderate increase	1451:1469	a moderate increase in hyaluronan	1451:1483	Aging resulted in a significant reduction in skin hydration and sulfated GAGs, a moderate increase in hyaluronan, and a corresponding, reduced VEF for albumin and the other macromolecular probes.
25362136	6	68	theme	isoelectric	1270:1280	arg1	pI					1289:1290	pI	1289:1290	pI	1289:1290	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	6	68	theme	isoelectric	1270:1280	arg1	point					1282:1286	probe isoelectric point	1264:1286	probe isoelectric point (pI)	1264:1291	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	3	69	theme	study	428:432	arg1	purpose					413:419	The purpose	409:419	The purpose of the study	409:432	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	3	70	theme	charge	563:568	arg1	span					522:525	a span	520:525	a span of molecular weights and electrical charge	520:568	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	8	71	theme	aged	1620:1623	arg1	skin					1625:1628	aged skin	1620:1628	aged skin	1620:1628	Our findings suggest that the changes in the ECM in aged skin may result in delayed adjustments of fluid perturbations and reduced ability for salt storage.
25362136	6	72	theme	probe	1264:1268	arg1	pI					1289:1290	pI	1289:1290	pI	1289:1290	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	6	72	theme	probe	1264:1268	arg1	point					1282:1286	probe isoelectric point	1264:1286	probe isoelectric point (pI)	1264:1291	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	5	73	theme	extracellular	1007:1019	arg1	phase					1027:1031	the extracellular fluid phase	1003:1031	the extracellular fluid phase	1003:1031	Albumin was excluded from 55±4% (n=8) of the extracellular fluid phase.
25362136	6	74	theme	fractional	1133:1142	arg1	volume					1153:1158	fractional excluded volume	1133:1158	fractional excluded volume (VEF)	1133:1164	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	6	74	theme	fractional	1133:1142	arg1	VEF					1161:1163	VEF	1161:1163	VEF	1161:1163	There was a highly significant, positive correlation between probe Stokes-Einstein (SE) radius and fractional excluded volume (VEF), described by VEF=0.078·SE radius+0.269 (P<0.001), and oppositely, a negative correlation between probe isoelectric point (pI) and exclusion for proteins with comparable size, VEF=-0.036·pI+0.719 (P=0.04).
25362136	3	75	theme	rat	488:490	arg1	skin					492:495	rat skin	488:495	rat skin	488:495	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	3	76	from	skin	492:495	arg1	range					453:457	the range	449:457	the range of interstitial exclusion in rat skin	449:495	The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular weights and electrical charge and also to test if a change in interstitial composition, occurring as a consequence of aging, affected exclusion.
25362136	1	77	theme	available	224:232	arg1	space					218:222	the space	214:222	the space available	214:232	Collagen and glycosaminoglycans (GAGs) constituting the ECM may limit the space available and thus exclude macromolecules from a fraction of the interstitial fluid (IF) phase.
28504125	3	0	theme	better	551:556	arg1	properties					569:578	better mechanical properties	551:578	better mechanical properties	551:578	TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film.
28504125	1	1	theme	food-borne	277:286	arg1	microbes					299:306	food-borne pathogenic microbes	277:306	food-borne pathogenic microbes	277:306	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	0	2	theme	visible	90:96	arg1	light					98:102	visible light	90:102	visible light for food packaging applications	90:134	Preparation of chitosan-TiO2 composite film with efficient antimicrobial activities under visible light for food packaging applications.
28504125	3	3	theme	mechanical	558:567	arg1	properties					569:578	better mechanical properties	551:578	better mechanical properties	551:578	TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film.
28504125	1	4	theme	pathogenic	288:297	arg1	microbes					299:306	food-borne pathogenic microbes	277:306	food-borne pathogenic microbes	277:306	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	0	5	theme	food	108:111	arg1	applications					123:134	food packaging applications	108:134	food packaging applications	108:134	Preparation of chitosan-TiO2 composite film with efficient antimicrobial activities under visible light for food packaging applications.
28504125	6	6	theme	chitosan-TiO2	973:985	arg1	film					987:990	The prepared chitosan-TiO2 film	960:990	The prepared chitosan-TiO2 film	960:990	The prepared chitosan-TiO2 film was tested for packaging red grapes to prevent microbial infection and extend their shelf life.
28504125	7	7	theme	food	1176:1179	arg1	applications					1191:1202	food packaging applications	1176:1202	food packaging applications	1176:1202	Results were positive, stressing the potential of the novel bio-nano composite film for food packaging applications.
28504125	3	8	theme	decreased	588:596	arg1	transmittance					604:616	decreased light transmittance	588:616	decreased light transmittance in visible light region of the composite film	588:662	TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film.
28504125	4	9	theme	Candida	806:812	arg1	albicans					814:821	Candida albicans	806:821	Candida albicans	806:821	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	4	9	theme	Candida	806:812	arg1	strains					751:757	four tested strains	739:757	four tested strains	739:757	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	6	10	theme	prepared	964:971	arg1	film					987:990	The prepared chitosan-TiO2 film	960:990	The prepared chitosan-TiO2 film	960:990	The prepared chitosan-TiO2 film was tested for packaging red grapes to prevent microbial infection and extend their shelf life.
28504125	7	11	theme	packaging	1181:1189	arg1	applications					1191:1202	food packaging applications	1176:1202	food packaging applications	1176:1202	Results were positive, stressing the potential of the novel bio-nano composite film for food packaging applications.
28504125	6	12	theme	packaging	1007:1015	arg1	grapes					1021:1026	packaging red grapes	1007:1026	packaging red grapes	1007:1026	The prepared chitosan-TiO2 film was tested for packaging red grapes to prevent microbial infection and extend their shelf life.
28504125	3	13	theme	light	598:602	arg1	transmittance					604:616	decreased light transmittance	588:616	decreased light transmittance in visible light region of the composite film	588:662	TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film.
28504125	7	14	theme	film	1167:1170	arg1	potential					1125:1133	the potential	1121:1133	the potential of the novel bio-nano composite film for food packaging applications	1121:1202	Results were positive, stressing the potential of the novel bio-nano composite film for food packaging applications.
28504125	4	15	theme	tested	744:749	arg1	albicans					814:821	Candida albicans	806:821	Candida albicans	806:821	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	4	15	theme	tested	744:749	arg1	strains					751:757	four tested strains	739:757	four tested strains	739:757	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	4	15	theme	tested	744:749	arg1	niger					840:844	Aspergillus niger	828:844	Aspergillus niger	828:844	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	4	15	theme	tested	744:749	arg1	coli					777:780	i.e. Escherichia coli	760:780	i.e. Escherichia coli	760:780	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	4	15	theme	tested	744:749	arg1	aureus					798:803	Staphylococcus aureus	783:803	Staphylococcus aureus	783:803	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	5	16	theme	cellular	914:921	arg1	substances					923:932	cellular substances	914:932	cellular substances	914:932	It moreover provoked the leakage of cellular substances through damaged membrane.
28504125	0	17	theme	packaging	113:121	arg1	applications					123:134	food packaging applications	108:134	food packaging applications	108:134	Preparation of chitosan-TiO2 composite film with efficient antimicrobial activities under visible light for food packaging applications.
28504125	6	18	theme	shelf	1076:1080	arg1	life					1082:1085	their shelf life	1070:1085	their shelf life	1070:1085	The prepared chitosan-TiO2 film was tested for packaging red grapes to prevent microbial infection and extend their shelf life.
28504125	1	19	from	incorporation	140:152	arg1	chitosan					177:184	chitosan	177:184	chitosan	177:184	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	5	20	theme	substances	923:932	arg1	leakage					903:909	the leakage	899:909	the leakage of cellular substances through damaged membrane	899:957	It moreover provoked the leakage of cellular substances through damaged membrane.
28504125	7	21	theme	bio-nano	1148:1155	arg1	film					1167:1170	the novel bio-nano composite film	1138:1170	the novel bio-nano composite film	1138:1170	Results were positive, stressing the potential of the novel bio-nano composite film for food packaging applications.
28504125	3	22	theme	visible	621:627	arg1	region					635:640	visible light region	621:640	visible light region of the composite film	621:662	TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film.
28504125	1	23	theme	chitosan-TiO2	189:201	arg1	film					213:216	a chitosan-TiO2 composite film	187:216	a chitosan-TiO2 composite film	187:216	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	1	23	theme	chitosan-TiO2	189:201	arg1	material					354:361	a promising food packaging material	327:361	a promising food packaging material	327:361	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	7	24	theme	composite	1157:1165	arg1	film					1167:1170	the novel bio-nano composite film	1138:1170	the novel bio-nano composite film	1138:1170	Results were positive, stressing the potential of the novel bio-nano composite film for food packaging applications.
28504125	3	25	theme	light	629:633	arg1	region					635:640	visible light region	621:640	visible light region of the composite film	621:662	TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film.
28504125	4	26	theme	Aspergillus	828:838	arg1	strains					751:757	four tested strains	739:757	four tested strains	739:757	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	4	26	theme	Aspergillus	828:838	arg1	niger					840:844	Aspergillus niger	828:844	Aspergillus niger	828:844	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	2	27	theme	TiO2	418:421	arg1	nano-powder					423:433	the TiO2 nano-powder	414:433	the TiO2 nano-powder	414:433	Scanning electron microscopy analysis showed that the TiO2 nano-powder was successfully and uniformly dispersed into the chitosan matrix.
28504125	0	28	theme	composite	29:37	arg1	film					39:42	chitosan-TiO2 composite film	15:42	chitosan-TiO2 composite film	15:42	Preparation of chitosan-TiO2 composite film with efficient antimicrobial activities under visible light for food packaging applications.
28504125	6	29	theme	microbial	1039:1047	arg1	infection					1049:1057	microbial infection	1039:1057	microbial infection	1039:1057	The prepared chitosan-TiO2 film was tested for packaging red grapes to prevent microbial infection and extend their shelf life.
28504125	0	30	theme	chitosan-TiO2	15:27	arg1	film					39:42	chitosan-TiO2 composite film	15:42	chitosan-TiO2 composite film	15:42	Preparation of chitosan-TiO2 composite film with efficient antimicrobial activities under visible light for food packaging applications.
28504125	7	31	theme	novel	1142:1146	arg1	film					1167:1170	the novel bio-nano composite film	1138:1170	the novel bio-nano composite film	1138:1170	Results were positive, stressing the potential of the novel bio-nano composite film for food packaging applications.
28504125	4	32	theme	antimicrobial	708:720	arg1	activity					722:729	efficient antimicrobial activity	698:729	efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger	698:844	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	1	33	theme	composite	203:211	arg1	film					213:216	a chitosan-TiO2 composite film	187:216	a chitosan-TiO2 composite film	187:216	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	1	33	theme	composite	203:211	arg1	material					354:361	a promising food packaging material	327:361	a promising food packaging material	327:361	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	3	34	theme	TiO2	502:505	arg1	addition					507:514	TiO2 addition	502:514	TiO2 addition	502:514	TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film.
28504125	0	35	theme	film	39:42	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan-TiO2 composite film with efficient antimicrobial activities under visible light for food packaging applications.	0:135	Preparation of chitosan-TiO2 composite film with efficient antimicrobial activities under visible light for food packaging applications.
28504125	1	36	theme	promising	329:337	arg1	film					213:216	a chitosan-TiO2 composite film	187:216	a chitosan-TiO2 composite film	187:216	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	1	36	theme	promising	329:337	arg1	material					354:361	a promising food packaging material	327:361	a promising food packaging material	327:361	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	2	37	theme	chitosan	485:492	arg1	matrix					494:499	the chitosan matrix	481:499	the chitosan matrix	481:499	Scanning electron microscopy analysis showed that the TiO2 nano-powder was successfully and uniformly dispersed into the chitosan matrix.
28504125	4	38	theme	efficient	698:706	arg1	activity					722:729	efficient antimicrobial activity	698:729	efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger	698:844	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	0	39	theme	antimicrobial	59:71	arg1	activities					73:82	efficient antimicrobial activities	49:82	efficient antimicrobial activities under visible light for food packaging applications	49:134	Preparation of chitosan-TiO2 composite film with efficient antimicrobial activities under visible light for food packaging applications.
28504125	1	40	theme	food	339:342	arg1	film					213:216	a chitosan-TiO2 composite film	187:216	a chitosan-TiO2 composite film	187:216	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	1	40	theme	food	339:342	arg1	material					354:361	a promising food packaging material	327:361	a promising food packaging material	327:361	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	4	41	contain	possessed	688:696	arg1	film					683:686	The chitosan-TiO2 film	665:686	The chitosan-TiO2 film	665:686	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	4	41	contain	possessed	688:696	arg2	activity					722:729	efficient antimicrobial activity	698:729	efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger	698:844	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	2	42	theme	microscopy	382:391	arg1	analysis					393:400	Scanning electron microscopy analysis	364:400	Scanning electron microscopy analysis	364:400	Scanning electron microscopy analysis showed that the TiO2 nano-powder was successfully and uniformly dispersed into the chitosan matrix.
28504125	0	43	theme	efficient	49:57	arg1	activities					73:82	efficient antimicrobial activities	49:82	efficient antimicrobial activities under visible light for food packaging applications	49:134	Preparation of chitosan-TiO2 composite film with efficient antimicrobial activities under visible light for food packaging applications.
28504125	1	44	theme	packaging	344:352	arg1	film					213:216	a chitosan-TiO2 composite film	187:216	a chitosan-TiO2 composite film	187:216	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	1	44	theme	packaging	344:352	arg1	material					354:361	a promising food packaging material	327:361	a promising food packaging material	327:361	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	3	45	theme	enhanced	523:530	arg1	hydrophilicity					532:545	enhanced hydrophilicity	523:545	enhanced hydrophilicity	523:545	TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film.
28504125	2	46	theme	electron	373:380	arg1	microscopy					382:391	Scanning electron microscopy	364:391	Scanning electron microscopy analysis	364:400	Scanning electron microscopy analysis showed that the TiO2 nano-powder was successfully and uniformly dispersed into the chitosan matrix.
28504125	3	47	from	transmittance	604:616	arg1	region					635:640	visible light region	621:640	visible light region of the composite film	621:662	TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film.
28504125	4	48	theme	%	854:854	arg1	sterilization					856:868	100% sterilization	851:868	100% sterilization in 12h	851:875	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	3	49	theme	composite	649:657	arg1	film					659:662	the composite film	645:662	the composite film	645:662	TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film.
28504125	0	50	with	Preparation	0:10	arg1	activities					73:82	efficient antimicrobial activities	49:82	efficient antimicrobial activities under visible light for food packaging applications	49:134	Preparation of chitosan-TiO2 composite film with efficient antimicrobial activities under visible light for food packaging applications.
28504125	2	51	theme	Scanning	364:371	arg1	microscopy					382:391	Scanning electron microscopy	364:391	Scanning electron microscopy analysis	364:400	Scanning electron microscopy analysis showed that the TiO2 nano-powder was successfully and uniformly dispersed into the chitosan matrix.
28504125	5	52	theme	damaged	942:948	arg1	membrane					950:957	damaged membrane	942:957	damaged membrane	942:957	It moreover provoked the leakage of cellular substances through damaged membrane.
28504125	1	53	theme	efficient	236:244	arg1	activity					260:267	efficient antimicrobial activity	236:267	efficient antimicrobial activity against food-borne pathogenic microbes	236:306	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	4	54	theme	100	851:853	arg1	%					854:854	%	854:854	%	854:854	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	1	55	theme	antimicrobial	246:258	arg1	activity					260:267	efficient antimicrobial activity	236:267	efficient antimicrobial activity against food-borne pathogenic microbes	236:306	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	3	56	theme	film	659:662	arg1	region					635:640	visible light region	621:640	visible light region of the composite film	621:662	TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film.
28504125	4	57	from	sterilization	856:868	arg1	12h					873:875	12h	873:875	12h	873:875	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	1	58	theme	TiO2	157:160	arg1	nano-powder					162:172	TiO2 nano-powder	157:172	TiO2 nano-powder	157:172	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	4	59	theme	chitosan-TiO2	669:681	arg1	film					683:686	The chitosan-TiO2 film	665:686	The chitosan-TiO2 film	665:686	The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h.
28504125	1	60	theme	nano-powder	162:172	arg1	incorporation					140:152	incorporation	140:152	incorporation of TiO2 nano-powder in chitosan	140:184	By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material.
28504125	6	61	theme	red	1017:1019	arg1	grapes					1021:1026	packaging red grapes	1007:1026	packaging red grapes	1007:1026	The prepared chitosan-TiO2 film was tested for packaging red grapes to prevent microbial infection and extend their shelf life.
29233912	12	0	link	N-linked	1666:1673	arg1	glycans					1675:1681	multiple N-linked glycans	1657:1681	multiple N-linked glycans of Hex5HexNAc2 structure	1657:1706	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	15	1	from	pathway	2186:2192	arg1	parasite					2202:2209	this parasite	2197:2209	this parasite	2197:2209	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	11	2	theme	vaginalis	1431:1439	arg1	pathway					1451:1457	the T. vaginalis secretory pathway	1424:1457	the T. vaginalis secretory pathway	1424:1457	Therefore, we used β-amylases as a model to investigate the T. vaginalis secretory pathway.
29233912	14	3	from	secretome	1874:1882	arg1	absent					1858:1863	absent	1858:1863	absent	1858:1863	Another two β-amylases (BA3 and BA4), which are encoded in the T. vaginalis genome but absent from the secretome, were targeted to the lysosomal compartment.
29233912	1	4	theme	fundamental	161:171	arg1	role					173:176	a fundamental role	159:176	a fundamental role	159:176	The secretion of virulence factors by parasitic protists into the host environment plays a fundamental role in multifactorial host-parasite interactions.
29233912	11	5	used	used	1382:1385	arg2	we					1379:1380	we	1379:1380	we	1379:1380	Therefore, we used β-amylases as a model to investigate the T. vaginalis secretory pathway.
29233912	4	6	theme	temperature-dependent	615:635	arg1	process					637:643	a time- and temperature-dependent process	603:643	a time- and temperature-dependent process	603:643	In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins.
29233912	4	6	theme	temperature-dependent	615:635	arg1	secretion					590:598	secretion	590:598	secretion	590:598	In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins.
29233912	9	7	theme	soluble	1108:1114	arg1	proteins					1116:1123	the soluble proteins	1104:1123	the soluble proteins	1104:1123	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	1	8	theme	parasitic	108:116	arg1	protists					118:125	parasitic protists	108:125	parasitic protists	108:125	The secretion of virulence factors by parasitic protists into the host environment plays a fundamental role in multifactorial host-parasite interactions.
29233912	13	9	theme	brefeldin	1740:1748	arg1	A					1750:1750	brefeldin A	1740:1750	brefeldin A	1740:1750	The secretion was inhibited by brefeldin A but not by FLI-06.
29233912	12	10	dep	β-amylases	1485:1494	arg1	BA2					1505:1507	BA2	1505:1507	BA2	1505:1507	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	12	10	dep	β-amylases	1485:1494	arg1	BA1					1497:1499	BA1	1497:1499	BA1	1497:1499	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	12	10	dep	β-amylases	1485:1494	arg1	β-amylases					1485:1494	two β-amylases	1481:1494	two β-amylases (BA1 and BA2)	1481:1508	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	2	11	theme	Several	224:230	arg1	proteins					241:248	Several effector proteins	224:248	Several effector proteins	224:248	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	14	12	theme	T.	1834:1835	arg1	genome					1847:1852	the T. vaginalis genome	1830:1852	the T. vaginalis genome	1830:1852	Another two β-amylases (BA3 and BA4), which are encoded in the T. vaginalis genome but absent from the secretome, were targeted to the lysosomal compartment.
29233912	10	13	theme	bioinformatic	1255:1267	arg1	tools					1269:1273	current bioinformatic tools	1247:1273	current bioinformatic tools	1247:1273	Interestingly, current bioinformatic tools predicted the secretory signal in only 18% of the identified T. vaginalis-secreted proteins.
29233912	7	14	theme	transmembrane	971:983	arg1	helixes					985:991	transmembrane helixes	971:991	transmembrane helixes	971:991	Approximately half of the secreted proteins were predicted to possess transmembrane helixes.
29233912	10	15	theme	identified	1325:1334	arg1	proteins					1358:1365	the identified T. vaginalis-secreted proteins	1321:1365	the identified T. vaginalis-secreted proteins	1321:1365	Interestingly, current bioinformatic tools predicted the secretory signal in only 18% of the identified T. vaginalis-secreted proteins.
29233912	4	16	theme	secreted	671:678	arg1	proteins					680:687	secreted proteins	671:687	secreted proteins	671:687	In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins.
29233912	1	17	theme	host	136:139	arg1	environment					141:151	the host environment	132:151	the host environment	132:151	The secretion of virulence factors by parasitic protists into the host environment plays a fundamental role in multifactorial host-parasite interactions.
29233912	12	18	theme	Hex5HexNAc2	1686:1696	arg1	structure					1698:1706	Hex5HexNAc2 structure	1686:1706	Hex5HexNAc2 structure	1686:1706	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	10	19	theme	secretory	1289:1297	arg1	signal					1299:1304	the secretory signal	1285:1304	the secretory signal	1285:1304	Interestingly, current bioinformatic tools predicted the secretory signal in only 18% of the identified T. vaginalis-secreted proteins.
29233912	7	20	theme	secreted	927:934	arg1	proteins					936:943	the secreted proteins	923:943	the secreted proteins	923:943	Approximately half of the secreted proteins were predicted to possess transmembrane helixes.
29233912	15	21	theme	proteins	2047:2054	arg1	reference					2076:2084	a reference	2074:2084	a reference for future comparative studies	2074:2115	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	15	21	theme	proteins	2047:2054	arg1	set					2016:2018	a comprehensive set	2000:2018	a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies	2000:2115	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	15	21	theme	proteins	2047:2054	arg1	proteins					2047:2054	constitutively secreted proteins	2023:2054	constitutively secreted proteins that can serve as a reference for future comparative studies	2023:2115	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	12	22	theme	multiple	1657:1664	arg1	glycans					1675:1681	multiple N-linked glycans	1657:1681	multiple N-linked glycans of Hex5HexNAc2 structure	1657:1706	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	12	23	theme	endoplasmic	1544:1554	arg1	pathways					1575:1582	the classical endoplasmic reticulum-to-Golgi pathways	1530:1582	the classical endoplasmic reticulum-to-Golgi pathways	1530:1582	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	5	24	theme	extracellular	720:732	arg1	proteins					734:741	2 072 extracellular proteins	714:741	2 072 extracellular proteins	714:741	In total, we identified 2 072 extracellular proteins, 89 of which displayed significant quantitative increases over time at 37 °C.
29233912	8	25	theme	leishmaniolysin-like	1046:1065	arg1	metallopeptidases					1067:1083	leishmaniolysin-like metallopeptidases	1046:1083	leishmaniolysin-like metallopeptidases	1046:1083	These proteins mainly include putative adhesins and leishmaniolysin-like metallopeptidases.
29233912	0	26	theme	β-amylases	58:67	arg1	study					49:53	Case study	44:53	Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases.	0:68	Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases.
29233912	0	27	theme	Dynamic	0:6	arg1	secretome					8:16	Dynamic secretome	0:16	Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases.	0:68	Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases.
29233912	9	28	theme	proteins	1116:1123	arg1	half					1096:1099	The other half	1086:1099	The other half of the soluble proteins	1086:1123	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	3	29	theme	protein	449:455	arg1	secretion					457:465	protein secretion	449:465	protein secretion	449:465	However, a comprehensive profiling of the T. vaginalis secretome remains elusive, as do the mechanisms of protein secretion.
29233912	6	30	theme	secreted	840:847	arg1	proteins					849:856	These 89 bona fide secreted proteins	821:856	These 89 bona fide secreted proteins	821:856	These 89 bona fide secreted proteins were sorted into 13 functional categories.
29233912	1	31	theme	factors	97:103	arg1	secretion					74:82	The secretion	70:82	The secretion of virulence factors by parasitic protists into the host environment	70:151	The secretion of virulence factors by parasitic protists into the host environment plays a fundamental role in multifactorial host-parasite interactions.
29233912	4	32	theme	T.	549:550	arg1	secretome					562:570	the T. vaginalis secretome	545:570	the T. vaginalis secretome	545:570	In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins.
29233912	9	33	theme	several	1133:1139	arg1	proteins					1206:1213	pore-forming proteins	1193:1213	pore-forming proteins	1193:1213	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	33	theme	several	1133:1139	arg1	β-amylases					1220:1229	β-amylases	1220:1229	β-amylases	1220:1229	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	33	theme	several	1133:1139	arg1	factors					1167:1173	several novel potential virulence factors	1133:1173	several novel potential virulence factors	1133:1173	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	33	theme	several	1133:1139	arg1	DNaseII					1184:1190	DNaseII	1184:1190	DNaseII	1184:1190	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	0	34	theme	vaginalis	33:41	arg1	secretome					8:16	Dynamic secretome	0:16	Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases.	0:68	Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases.
29233912	6	35	theme	bona	830:833	arg1	proteins					849:856	These 89 bona fide secreted proteins	821:856	These 89 bona fide secreted proteins	821:856	These 89 bona fide secreted proteins were sorted into 13 functional categories.
29233912	15	36	theme	future	2090:2095	arg1	studies					2109:2115	future comparative studies	2090:2115	future comparative studies	2090:2115	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	3	37	theme	vaginalis	388:396	arg1	secretome					398:406	the T. vaginalis secretome	381:406	the T. vaginalis secretome	381:406	However, a comprehensive profiling of the T. vaginalis secretome remains elusive, as do the mechanisms of protein secretion.
29233912	4	38	theme	quantitative	518:529	arg1	MS					531:532	high-resolution label-free quantitative MS	491:532	high-resolution label-free quantitative MS	491:532	In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins.
29233912	15	39	theme	in	1957:1958	arg1	conditions					1966:1975	defined in vitro conditions	1949:1975	defined in vitro conditions	1949:1975	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	9	40	theme	potential	1147:1155	arg1	proteins					1206:1213	pore-forming proteins	1193:1213	pore-forming proteins	1193:1213	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	40	theme	potential	1147:1155	arg1	β-amylases					1220:1229	β-amylases	1220:1229	β-amylases	1220:1229	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	40	theme	potential	1147:1155	arg1	factors					1167:1173	several novel potential virulence factors	1133:1173	several novel potential virulence factors	1133:1173	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	40	theme	potential	1147:1155	arg1	DNaseII					1184:1190	DNaseII	1184:1190	DNaseII	1184:1190	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	5	41	theme	quantitative	778:789	arg1	increases					791:799	significant quantitative increases	766:799	significant quantitative increases	766:799	In total, we identified 2 072 extracellular proteins, 89 of which displayed significant quantitative increases over time at 37 °C.
29233912	10	42	theme	vaginalis-secreted	1339:1356	arg1	proteins					1358:1365	the identified T. vaginalis-secreted proteins	1321:1365	the identified T. vaginalis-secreted proteins	1321:1365	Interestingly, current bioinformatic tools predicted the secretory signal in only 18% of the identified T. vaginalis-secreted proteins.
29233912	2	43	theme	urogenital	325:334	arg1	tract					336:340	the urogenital tract	321:340	the urogenital tract	321:340	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	15	44	theme	classical	2166:2174	arg1	pathway					2186:2192	the classical secretory pathway	2162:2192	the classical secretory pathway in this parasite	2162:2209	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	7	45	contain	possess	963:969	arg2	helixes					985:991	transmembrane helixes	971:991	transmembrane helixes	971:991	Approximately half of the secreted proteins were predicted to possess transmembrane helixes.
29233912	7	45	contain	possess	963:969	arg1	half					915:918	Approximately half	901:918	Approximately half of the secreted proteins	901:943	Approximately half of the secreted proteins were predicted to possess transmembrane helixes.
29233912	15	46	theme	first	2138:2142	arg1	information					2144:2154	the first information	2134:2154	the first information about the classical secretory pathway in this parasite	2134:2209	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	4	47	theme	label-free	507:516	arg1	MS					531:532	high-resolution label-free quantitative MS	491:532	high-resolution label-free quantitative MS	491:532	In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins.
29233912	6	48	theme	functional	878:887	arg1	categories					889:898	13 functional categories	875:898	13 functional categories	875:898	These 89 bona fide secreted proteins were sorted into 13 functional categories.
29233912	11	49	theme	T.	1428:1429	arg1	pathway					1451:1457	the T. vaginalis secretory pathway	1424:1457	the T. vaginalis secretory pathway	1424:1457	Therefore, we used β-amylases as a model to investigate the T. vaginalis secretory pathway.
29233912	9	50	theme	pore-forming	1193:1204	arg1	proteins					1206:1213	pore-forming proteins	1193:1213	pore-forming proteins	1193:1213	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	2	51	theme	Trichomonas	278:288	arg1	vaginalis					290:298	Trichomonas vaginalis	278:298	Trichomonas vaginalis	278:298	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	2	51	theme	Trichomonas	278:288	arg1	tract					336:340	the urogenital tract	321:340	the urogenital tract	321:340	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	2	51	theme	Trichomonas	278:288	arg1	parasite					309:316	a human parasite	301:316	a human parasite of the urogenital tract	301:340	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	0	52	theme	Case	44:47	arg1	study					49:53	Case study	44:53	Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases.	0:68	Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases.
29233912	2	53	theme	human	303:307	arg1	vaginalis					290:298	Trichomonas vaginalis	278:298	Trichomonas vaginalis	278:298	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	2	53	theme	human	303:307	arg1	tract					336:340	the urogenital tract	321:340	the urogenital tract	321:340	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	2	53	theme	human	303:307	arg1	parasite					309:316	a human parasite	301:316	a human parasite of the urogenital tract	301:340	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	1	54	theme	multifactorial	181:194	arg1	interactions					210:221	multifactorial host-parasite interactions	181:221	multifactorial host-parasite interactions	181:221	The secretion of virulence factors by parasitic protists into the host environment plays a fundamental role in multifactorial host-parasite interactions.
29233912	9	55	theme	virulence	1157:1165	arg1	proteins					1206:1213	pore-forming proteins	1193:1213	pore-forming proteins	1193:1213	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	55	theme	virulence	1157:1165	arg1	β-amylases					1220:1229	β-amylases	1220:1229	β-amylases	1220:1229	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	55	theme	virulence	1157:1165	arg1	factors					1167:1173	several novel potential virulence factors	1133:1173	several novel potential virulence factors	1133:1173	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	55	theme	virulence	1157:1165	arg1	DNaseII					1184:1190	DNaseII	1184:1190	DNaseII	1184:1190	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	14	56	dep	β-amylases	1783:1792	arg1	β-amylases					1783:1792	Another two β-amylases	1771:1792	Another two β-amylases (BA3 and BA4)	1771:1806	Another two β-amylases (BA3 and BA4), which are encoded in the T. vaginalis genome but absent from the secretome, were targeted to the lysosomal compartment.
29233912	14	56	dep	β-amylases	1783:1792	arg1	BA4					1803:1805	BA4	1803:1805	BA4	1803:1805	Another two β-amylases (BA3 and BA4), which are encoded in the T. vaginalis genome but absent from the secretome, were targeted to the lysosomal compartment.
29233912	14	56	dep	β-amylases	1783:1792	arg1	BA3					1795:1797	BA3	1795:1797	BA3	1795:1797	Another two β-amylases (BA3 and BA4), which are encoded in the T. vaginalis genome but absent from the secretome, were targeted to the lysosomal compartment.
29233912	15	57	theme	comparative	2097:2107	arg1	studies					2109:2115	future comparative studies	2090:2115	future comparative studies	2090:2115	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	2	58	theme	effector	232:239	arg1	proteins					241:248	Several effector proteins	224:248	Several effector proteins	224:248	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	14	59	theme	vaginalis	1837:1845	arg1	genome					1847:1852	the T. vaginalis genome	1830:1852	the T. vaginalis genome	1830:1852	Another two β-amylases (BA3 and BA4), which are encoded in the T. vaginalis genome but absent from the secretome, were targeted to the lysosomal compartment.
29233912	12	60	theme	BA1	1604:1606	arg1	case					1596:1599	the case	1592:1599	the case of BA1	1592:1606	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	15	61	theme	secretory	2176:2184	arg1	pathway					2186:2192	the classical secretory pathway	2162:2192	the classical secretory pathway in this parasite	2162:2209	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	10	62	theme	current	1247:1253	arg1	tools					1269:1273	current bioinformatic tools	1247:1273	current bioinformatic tools	1247:1273	Interestingly, current bioinformatic tools predicted the secretory signal in only 18% of the identified T. vaginalis-secreted proteins.
29233912	5	63	theme	proteins	734:741	arg1	89					744:745	89	744:745	89	744:745	In total, we identified 2 072 extracellular proteins, 89 of which displayed significant quantitative increases over time at 37 °C.
29233912	5	63	theme	proteins	734:741	arg1	proteins					734:741	2 072 extracellular proteins	714:741	2 072 extracellular proteins	714:741	In total, we identified 2 072 extracellular proteins, 89 of which displayed significant quantitative increases over time at 37 °C.
29233912	8	64	theme	putative	1024:1031	arg1	adhesins					1033:1040	putative adhesins	1024:1040	putative adhesins	1024:1040	These proteins mainly include putative adhesins and leishmaniolysin-like metallopeptidases.
29233912	15	65	theme	defined	1949:1955	arg1	conditions					1966:1975	defined in vitro conditions	1949:1975	defined in vitro conditions	1949:1975	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	12	66	theme	classical	1534:1542	arg1	pathways					1575:1582	the classical endoplasmic reticulum-to-Golgi pathways	1530:1582	the classical endoplasmic reticulum-to-Golgi pathways	1530:1582	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	4	67	theme	high-resolution	491:505	arg1	MS					531:532	high-resolution label-free quantitative MS	491:532	high-resolution label-free quantitative MS	491:532	In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins.
29233912	15	68	theme	secreted	2038:2045	arg1	reference					2076:2084	a reference	2074:2084	a reference for future comparative studies	2074:2115	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	15	68	theme	secreted	2038:2045	arg1	proteins					2047:2054	constitutively secreted proteins	2023:2054	constitutively secreted proteins that can serve as a reference for future comparative studies	2023:2115	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	12	69	theme	structure	1698:1706	arg1	glycans					1675:1681	multiple N-linked glycans	1657:1681	multiple N-linked glycans of Hex5HexNAc2 structure	1657:1706	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	12	70	theme	reticulum-to-Golgi	1556:1573	arg1	pathways					1575:1582	the classical endoplasmic reticulum-to-Golgi pathways	1530:1582	the classical endoplasmic reticulum-to-Golgi pathways	1530:1582	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	3	71	theme	comprehensive	354:366	arg1	profiling					368:376	a comprehensive profiling	352:376	a comprehensive profiling of the T. vaginalis secretome	352:406	However, a comprehensive profiling of the T. vaginalis secretome remains elusive, as do the mechanisms of protein secretion.
29233912	9	72	theme	other	1090:1094	arg1	half					1096:1099	The other half	1086:1099	The other half of the soluble proteins	1086:1123	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	5	73	theme	072	716:718	arg1	proteins					734:741	2 072 extracellular proteins	714:741	2 072 extracellular proteins	714:741	In total, we identified 2 072 extracellular proteins, 89 of which displayed significant quantitative increases over time at 37 °C.
29233912	7	74	theme	proteins	936:943	arg1	half					915:918	Approximately half	901:918	Approximately half of the secreted proteins	901:943	Approximately half of the secreted proteins were predicted to possess transmembrane helixes.
29233912	11	75	theme	secretory	1441:1449	arg1	pathway					1451:1457	the T. vaginalis secretory pathway	1424:1457	the T. vaginalis secretory pathway	1424:1457	Therefore, we used β-amylases as a model to investigate the T. vaginalis secretory pathway.
29233912	15	76	dep	in	1957:1958	arg1	vitro					1960:1964	vitro	1960:1964	vitro	1960:1964	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	4	77	used	used	486:489	arg2	we					483:484	we	483:484	we	483:484	In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins.
29233912	15	78	theme	comprehensive	2002:2014	arg1	reference					2076:2084	a reference	2074:2084	a reference for future comparative studies	2074:2115	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	15	78	theme	comprehensive	2002:2014	arg1	set					2016:2018	a comprehensive set	2000:2018	a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies	2000:2115	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	15	78	theme	comprehensive	2002:2014	arg1	proteins					2047:2054	constitutively secreted proteins	2023:2054	constitutively secreted proteins that can serve as a reference for future comparative studies	2023:2115	Collectively, under defined in vitro conditions, our analysis provides a comprehensive set of constitutively secreted proteins that can serve as a reference for future comparative studies, and it provides the first information about the classical secretory pathway in this parasite.
29233912	12	79	theme	N-linked	1666:1673	arg1	glycans					1675:1681	multiple N-linked glycans	1657:1681	multiple N-linked glycans of Hex5HexNAc2 structure	1657:1706	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	3	80	theme	T.	385:386	arg1	secretome					398:406	the T. vaginalis secretome	381:406	the T. vaginalis secretome	381:406	However, a comprehensive profiling of the T. vaginalis secretome remains elusive, as do the mechanisms of protein secretion.
29233912	10	81	theme	T.	1336:1337	arg1	proteins					1358:1365	the identified T. vaginalis-secreted proteins	1321:1365	the identified T. vaginalis-secreted proteins	1321:1365	Interestingly, current bioinformatic tools predicted the secretory signal in only 18% of the identified T. vaginalis-secreted proteins.
29233912	0	82	theme	Trichomonas	21:31	arg1	vaginalis					33:41	Trichomonas vaginalis	21:41	Trichomonas vaginalis	21:41	Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases.
29233912	1	83	theme	virulence	87:95	arg1	factors					97:103	virulence factors	87:103	virulence factors	87:103	The secretion of virulence factors by parasitic protists into the host environment plays a fundamental role in multifactorial host-parasite interactions.
29233912	4	84	theme	vaginalis	552:560	arg1	secretome					562:570	the T. vaginalis secretome	545:570	the T. vaginalis secretome	545:570	In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins.
29233912	3	85	theme	secretion	457:465	arg1	mechanisms					435:444	the mechanisms	431:444	the mechanisms of protein secretion	431:465	However, a comprehensive profiling of the T. vaginalis secretome remains elusive, as do the mechanisms of protein secretion.
29233912	6	86	dep	bona	830:833	arg1	fide					835:838	fide	835:838	fide	835:838	These 89 bona fide secreted proteins were sorted into 13 functional categories.
29233912	9	87	theme	novel	1141:1145	arg1	proteins					1206:1213	pore-forming proteins	1193:1213	pore-forming proteins	1193:1213	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	87	theme	novel	1141:1145	arg1	β-amylases					1220:1229	β-amylases	1220:1229	β-amylases	1220:1229	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	87	theme	novel	1141:1145	arg1	factors					1167:1173	several novel potential virulence factors	1133:1173	several novel potential virulence factors	1133:1173	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	9	87	theme	novel	1141:1145	arg1	DNaseII					1184:1190	DNaseII	1184:1190	DNaseII	1184:1190	The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases.
29233912	5	88	theme	significant	766:776	arg1	increases					791:799	significant quantitative increases	766:799	significant quantitative increases	766:799	In total, we identified 2 072 extracellular proteins, 89 of which displayed significant quantitative increases over time at 37 °C.
29233912	3	89	dep	do	428:429	arg1	as					425:426	as	425:426	as	425:426	However, a comprehensive profiling of the T. vaginalis secretome remains elusive, as do the mechanisms of protein secretion.
29233912	3	90	theme	secretome	398:406	arg1	profiling					368:376	a comprehensive profiling	352:376	a comprehensive profiling of the T. vaginalis secretome	352:406	However, a comprehensive profiling of the T. vaginalis secretome remains elusive, as do the mechanisms of protein secretion.
29233912	10	91	theme	proteins	1358:1365	arg1	%					1316:1316	only 18%	1309:1316	only 18% of the identified T. vaginalis-secreted proteins	1309:1365	Interestingly, current bioinformatic tools predicted the secretory signal in only 18% of the identified T. vaginalis-secreted proteins.
29233912	10	91	theme	proteins	1358:1365	arg1	proteins					1358:1365	the identified T. vaginalis-secreted proteins	1321:1365	the identified T. vaginalis-secreted proteins	1321:1365	Interestingly, current bioinformatic tools predicted the secretory signal in only 18% of the identified T. vaginalis-secreted proteins.
29233912	2	92	theme	tract	336:340	arg1	vaginalis					290:298	Trichomonas vaginalis	278:298	Trichomonas vaginalis	278:298	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	2	92	theme	tract	336:340	arg1	tract					336:340	the urogenital tract	321:340	the urogenital tract	321:340	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	2	92	theme	tract	336:340	arg1	parasite					309:316	a human parasite	301:316	a human parasite of the urogenital tract	301:340	Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract.
29233912	14	93	theme	lysosomal	1906:1914	arg1	compartment					1916:1926	the lysosomal compartment	1902:1926	the lysosomal compartment	1902:1926	Another two β-amylases (BA3 and BA4), which are encoded in the T. vaginalis genome but absent from the secretome, were targeted to the lysosomal compartment.
29233912	12	94	gly	glycosylated	1639:1650	arg1	protein					1628:1634	the protein	1624:1634	the protein	1624:1634	We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex5HexNAc2 structure.
29233912	1	95	theme	host-parasite	196:208	arg1	interactions					210:221	multifactorial host-parasite interactions	181:221	multifactorial host-parasite interactions	181:221	The secretion of virulence factors by parasitic protists into the host environment plays a fundamental role in multifactorial host-parasite interactions.
29233912	0	96	dep	secretome	8:16	arg1	study					49:53	Case study	44:53	Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases.	0:68	Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases.
29233912	4	97	theme	time-	605:609	arg1	process					637:643	a time- and temperature-dependent process	603:643	a time- and temperature-dependent process	603:643	In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins.
29233912	4	97	theme	time-	605:609	arg1	secretion					590:598	secretion	590:598	secretion	590:598	In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins.
25367521	12	0	theme	feline	1596:1601	arg1	cultures					1609:1616	feline fecal cultures	1596:1616	feline fecal cultures	1596:1616	Both FOS and LAC were added to feline fecal cultures at 2 g/L for 24 h incubation.
25367521	11	1	theme	LP	1489:1490	arg1	diet					1492:1495	LP diet	1489:1495	LP diet	1489:1495	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	11	2	with	LAC	1538:1540	arg1	addition					1455:1462	no addition	1452:1462	no addition of prebiotics (CTRL-HP)	1452:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	15	3	dep	level	2023:2027	arg1	mmol/L					2044:2049	40.9 vs. 32.6 mmol/L	2030:2049	40.9 vs. 32.6 mmol/L for LP and HP	2030:2063	Total VFA were influenced (P<0.05) by the protein level (40.9 vs. 32.6 mmol/L for LP and HP, respectively).
25367521	10	4	theme	second	1219:1224	arg1	study					1226:1230	a second study	1217:1230	a second study	1217:1230	In a second study, LAC and FOS were selected to be tested in the presence of 2 diets differing in their protein content.
25367521	18	5	contain	have	2433:2436	arg2	effects					2447:2453	negative effects	2438:2453	negative effects	2438:2453	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	18	5	contain	have	2433:2436	arg1	levels					2406:2411	high dietary protein levels	2385:2411	high dietary protein levels in a cat's diet	2385:2427	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	15	6	theme	Total	1973:1977	arg1	VFA					1979:1981	Total VFA	1973:1981	Total VFA	1973:1981	Total VFA were influenced (P<0.05) by the protein level (40.9 vs. 32.6 mmol/L for LP and HP, respectively).
25367521	11	7	with	diet	1528:1531	arg1	addition					1455:1462	no addition	1452:1462	no addition of prebiotics (CTRL-HP)	1452:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	13	8	dep	affected	1668:1675	arg1	P<0.05					1678:1683	P<0.05	1678:1683	P<0.05	1678:1683	Ammonia at 24 h was affected (P<0.05) by the protein level (36.2 vs. 50.2 mmol/L for LP and HP, respectively).
25367521	14	9	theme	incubation	1875:1884	arg1	h					1870:1870	6 and 24 h	1861:1870	6 and 24 h of incubation (P<0.05)	1861:1893	The CTRL-HPs resulted in a higher pH and increased concentrations of biogenic amines were found after 6 and 24 h of incubation (P<0.05); putrescine at 24 h showed an increase (P<0.05) in cultures treated with FOS.
25367521	16	10	theme	Clostridium	2134:2144	arg1	perfringens					2146:2156	increased Clostridium perfringens	2124:2156	increased Clostridium perfringens	2124:2156	At 24 h, the CTRL-HPs were associated with increased Clostridium perfringens and reduced Lactobacillus spp.
25367521	1	11	theme	substances	187:196	arg1	effect					162:167	the effect	158:167	the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota	158:284	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	18	12	theme	intestinal	2469:2478	arg1	environment					2480:2490	the animal intestinal environment	2458:2490	the animal intestinal environment	2458:2490	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	1	13	dep	composition	234:244	arg1	the					230:232	the	230:232	the	230:232	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	4	14	theme	fecal	600:604	arg1	cultures					606:613	feline fecal cultures	593:613	feline fecal cultures	593:613	Substrates were added to feline fecal cultures at 2 g/L for 24 h incubation.
25367521	14	15	from	h	1913:1913	arg1	putrescine					1896:1905	putrescine	1896:1905	putrescine at 24 h	1896:1913	The CTRL-HPs resulted in a higher pH and increased concentrations of biogenic amines were found after 6 and 24 h of incubation (P<0.05); putrescine at 24 h showed an increase (P<0.05) in cultures treated with FOS.
25367521	1	16	theme	present	119:125	arg1	study					127:131	the present study	115:131	the present study	115:131	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	12	17	theme	h	1634:1634	arg1	incubation					1636:1645	24 h incubation	1631:1645	24 h incubation	1631:1645	Both FOS and LAC were added to feline fecal cultures at 2 g/L for 24 h incubation.
25367521	11	18	with	prebiotics	1402:1411	arg1	addition					1455:1462	no addition	1452:1462	no addition of prebiotics (CTRL-HP)	1452:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	11	19	theme	prebiotics	1402:1411	arg1	addition					1390:1397	no addition	1387:1397	no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC	1387:1562	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	18	20	from	levels	2406:2411	arg1	diet					2424:2427	a cat's diet	2416:2427	a cat's diet	2416:2427	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	16	21	theme	reduced	2162:2168	arg1	spp					2184:2186	reduced Lactobacillus spp	2162:2186	reduced Lactobacillus spp	2162:2186	At 24 h, the CTRL-HPs were associated with increased Clostridium perfringens and reduced Lactobacillus spp.
25367521	18	22	theme	animal	2462:2467	arg1	environment					2480:2490	the animal intestinal environment	2458:2490	the animal intestinal environment	2458:2490	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	14	23	theme	biogenic	1828:1835	arg1	amines					1837:1842	biogenic amines	1828:1842	biogenic amines	1828:1842	The CTRL-HPs resulted in a higher pH and increased concentrations of biogenic amines were found after 6 and 24 h of incubation (P<0.05); putrescine at 24 h showed an increase (P<0.05) in cultures treated with FOS.
25367521	4	24	theme	24	628:629	arg1	h					631:631	h	631:631	h	631:631	Substrates were added to feline fecal cultures at 2 g/L for 24 h incubation.
25367521	0	25	theme	fecal	86:90	arg1	microbiota					92:101	feline fecal microbiota	79:101	feline fecal microbiota	79:101	In vitro effect of dietary protein level and nondigestible oligosaccharides on feline fecal microbiota.
25367521	18	26	theme	dietary	2390:2396	arg1	levels					2406:2411	high dietary protein levels	2385:2411	high dietary protein levels in a cat's diet	2385:2427	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	8	27	dep	%	1129:1129	arg1	P<0.05					1132:1137	P<0.05	1132:1137	-51%; P<0.05	1126:1137	Compared with the CTRL, total VFA were higher (P<0.05) in bottles containing CF (+41%), whereas the acetic to propionic acid ratio was reduced by LAC (-51%; P<0.05).
25367521	11	28	theme	diet	1528:1531	arg1	addition					1390:1397	no addition	1387:1397	no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC	1387:1562	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	8	29	theme	acetic	1075:1080	arg1	ratio					1100:1104	the acetic to propionic acid ratio	1071:1104	the acetic to propionic acid ratio	1071:1104	Compared with the CTRL, total VFA were higher (P<0.05) in bottles containing CF (+41%), whereas the acetic to propionic acid ratio was reduced by LAC (-51%; P<0.05).
25367521	3	30	theme	control	394:400	arg1	CTRL					408:411	CTRL	408:411	CTRL	408:411	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	30	theme	control	394:400	arg1	diet					402:405	control diet	394:405	control diet (CTRL)	394:412	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	0	31	theme	In	0:1	arg1	effect					9:14	In vitro effect	0:14	In vitro effect of dietary protein level and nondigestible	0:57	In vitro effect of dietary protein level and nondigestible oligosaccharides on feline fecal microbiota.
25367521	8	32	theme	propionic	1085:1093	arg1	acid					1095:1098	propionic acid	1085:1098	propionic acid	1085:1098	Compared with the CTRL, total VFA were higher (P<0.05) in bottles containing CF (+41%), whereas the acetic to propionic acid ratio was reduced by LAC (-51%; P<0.05).
25367521	7	33	dep	greater	893:899	arg1	P<0.05					902:907	P<0.05	902:907	P<0.05	902:907	Putrescine concentrations at 24 h were greater (P<0.05) in cultures treated with FOS (+90%), GOS (+96%), and LAC (+87%).
25367521	3	34	theme	gluconic	415:422	arg1	GA					430:431	GA	430:431	GA	430:431	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	34	theme	gluconic	415:422	arg1	acid					424:427	gluconic acid	415:427	gluconic acid (GA)	415:432	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	16	35	theme	increased	2124:2132	arg1	perfringens					2146:2156	increased Clostridium perfringens	2124:2156	increased Clostridium perfringens	2124:2156	At 24 h, the CTRL-HPs were associated with increased Clostridium perfringens and reduced Lactobacillus spp.
25367521	14	36	theme	increased	1800:1808	arg1	concentrations					1810:1823	increased concentrations	1800:1823	increased concentrations of biogenic amines	1800:1842	The CTRL-HPs resulted in a higher pH and increased concentrations of biogenic amines were found after 6 and 24 h of incubation (P<0.05); putrescine at 24 h showed an increase (P<0.05) in cultures treated with FOS.
25367521	0	37	theme	nondigestible	45:57	arg1	effect					9:14	In vitro effect	0:14	In vitro effect of dietary protein level and nondigestible	0:57	In vitro effect of dietary protein level and nondigestible oligosaccharides on feline fecal microbiota.
25367521	11	38	theme	low-protein	1360:1370	arg1	CTRL					1377:1380	low-protein (LP) CTRL	1360:1380	low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC	1360:1562	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	0	39	theme	dietary	19:25	arg1	level					35:39	dietary protein level	19:39	dietary protein level	19:39	In vitro effect of dietary protein level and nondigestible oligosaccharides on feline fecal microbiota.
25367521	1	40	theme	fecal	269:273	arg1	microbiota					275:284	feline fecal microbiota	262:284	feline fecal microbiota	262:284	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	10	41	theme	diets	1293:1297	arg1	presence					1279:1286	the presence	1275:1286	the presence of 2 diets differing in their protein content	1275:1332	In a second study, LAC and FOS were selected to be tested in the presence of 2 diets differing in their protein content.
25367521	9	42	dep	reduced	1181:1187	arg1	P<0.05					1190:1195	P<0.05	1190:1195	P<0.05	1190:1195	After 24 h, Enterobacteriaceae had been reduced (P<0.05) by LAC and PEC.
25367521	11	43	with	CTRL-HP	1507:1513	arg1	addition					1455:1462	no addition	1452:1462	no addition of prebiotics (CTRL-HP)	1452:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	17	44	theme	enterococci	2193:2203	arg1	P<0.05					2213:2218	P<0.05	2213:2218	P<0.05	2213:2218	and enterococci counts (P<0.05).
25367521	17	44	theme	enterococci	2193:2203	arg1	counts					2205:2210	enterococci counts	2193:2210	enterococci counts (P<0.05)	2193:2219	and enterococci counts (P<0.05).
25367521	18	45	theme	microbiota	2365:2374	arg1	activity					2335:2342	activity	2335:2342	activity	2335:2342	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	18	45	theme	microbiota	2365:2374	arg1	composition					2319:2329	composition	2319:2329	composition	2319:2329	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	11	46	with	FOS	1520:1522	arg1	addition					1455:1462	no addition	1452:1462	no addition of prebiotics (CTRL-HP)	1452:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	10	47	theme	protein	1318:1324	arg1	content					1326:1332	their protein content	1312:1332	their protein content	1312:1332	In a second study, LAC and FOS were selected to be tested in the presence of 2 diets differing in their protein content.
25367521	1	48	theme	dietary	204:210	arg1	levels					220:225	2 dietary protein levels	202:225	2 dietary protein levels	202:225	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	0	49	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro effect of dietary protein level and nondigestible oligosaccharides on feline fecal microbiota.
25367521	11	50	with	diet	1492:1495	arg1	addition					1455:1462	no addition	1452:1462	no addition of prebiotics (CTRL-HP)	1452:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	3	51	theme	carrot	435:440	arg1	fiber					442:446	carrot fiber	435:446	carrot fiber (CF)	435:451	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	51	theme	carrot	435:440	arg1	CF					449:450	CF	449:450	CF	449:450	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	1	52	theme	levels	220:225	arg1	effect					162:167	the effect	158:167	the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota	158:284	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	11	53	with	FOS	1502:1504	arg1	addition					1455:1462	no addition	1452:1462	no addition of prebiotics (CTRL-HP)	1452:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	11	54	theme	high-protein	1424:1435	arg1	CTRL					1442:1445	high-protein (HP) CTRL	1424:1445	high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP)	1424:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	11	54	theme	high-protein	1424:1435	arg1	HP					1438:1439	HP	1438:1439	HP	1438:1439	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	11	55	theme	diet	1492:1495	arg1	addition					1390:1397	no addition	1387:1397	no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC	1387:1562	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	0	56	theme	level	35:39	arg1	effect					9:14	In vitro effect	0:14	In vitro effect of dietary protein level and nondigestible	0:57	In vitro effect of dietary protein level and nondigestible oligosaccharides on feline fecal microbiota.
25367521	11	57	with	CTRL-HP	1547:1553	arg1	addition					1455:1462	no addition	1452:1462	no addition of prebiotics (CTRL-HP)	1452:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	15	58	dep	influenced	1988:1997	arg1	P<0.05					2000:2005	P<0.05	2000:2005	P<0.05	2000:2005	Total VFA were influenced (P<0.05) by the protein level (40.9 vs. 32.6 mmol/L for LP and HP, respectively).
25367521	11	59	theme	CTRL	1442:1445	arg1	addition					1390:1397	no addition	1387:1397	no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC	1387:1562	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	18	60	theme	different	2267:2275	arg1	prebiotics					2277:2286	different prebiotics	2267:2286	different prebiotics	2267:2286	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	11	61	theme	CTRL-HP	1507:1513	arg1	addition					1390:1397	no addition	1387:1397	no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC	1387:1562	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	18	62	from	study	2251:2255	arg1	results					2226:2232	The results	2222:2232	The results from the present study	2222:2255	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	11	63	theme	prebiotics	1467:1476	arg1	addition					1455:1462	no addition	1452:1462	no addition of prebiotics (CTRL-HP)	1452:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	6	64	theme	lower	828:832	arg1	pH					834:835	a lower pH	826:835	a lower pH	826:835	After 24 h, all treatments had resulted in a lower pH versus the CTRL.
25367521	3	65	theme	nondigestible	333:345	arg1	oligosaccharides					347:362	6 nondigestible oligosaccharides	331:362	6 nondigestible oligosaccharides	331:362	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	1	66	theme	prebiotic	177:185	arg1	substances					187:196	some prebiotic substances	172:196	some prebiotic substances	172:196	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	7	67	from	h	886:886	arg1	concentrations					865:878	Putrescine concentrations	854:878	Putrescine concentrations at 24 h	854:886	Putrescine concentrations at 24 h were greater (P<0.05) in cultures treated with FOS (+90%), GOS (+96%), and LAC (+87%).
25367521	15	68	theme	protein	2015:2021	arg1	level					2023:2027	the protein level	2011:2027	the protein level (40.9 vs. 32.6 mmol/L for LP and HP, respectively)	2011:2078	Total VFA were influenced (P<0.05) by the protein level (40.9 vs. 32.6 mmol/L for LP and HP, respectively).
25367521	8	69	dep	reduced	1110:1116	arg1	%					1129:1129	-51%	1126:1129	-51%; P<0.05	1126:1137	Compared with the CTRL, total VFA were higher (P<0.05) in bottles containing CF (+41%), whereas the acetic to propionic acid ratio was reduced by LAC (-51%; P<0.05).
25367521	8	70	dep	higher	1014:1019	arg1	P<0.05					1022:1027	P<0.05	1022:1027	P<0.05	1022:1027	Compared with the CTRL, total VFA were higher (P<0.05) in bottles containing CF (+41%), whereas the acetic to propionic acid ratio was reduced by LAC (-51%; P<0.05).
25367521	12	71	theme	24	1631:1632	arg1	h					1634:1634	h	1634:1634	h	1634:1634	Both FOS and LAC were added to feline fecal cultures at 2 g/L for 24 h incubation.
25367521	18	72	theme	negative	2438:2445	arg1	effects					2447:2453	negative effects	2438:2453	negative effects	2438:2453	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	11	73	with	LAC	1560:1562	arg1	addition					1455:1462	no addition	1452:1462	no addition of prebiotics (CTRL-HP)	1452:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	4	74	theme	feline	593:598	arg1	cultures					606:613	feline fecal cultures	593:613	feline fecal cultures	593:613	Substrates were added to feline fecal cultures at 2 g/L for 24 h incubation.
25367521	1	75	from	effect	162:167	arg1	activity					250:257	activity	250:257	activity	250:257	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	1	75	from	effect	162:167	arg1	composition					234:244	composition	234:244	composition	234:244	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	1	76	theme	study	127:131	arg1	aim					108:110	The aim	104:110	The aim of the present study	104:131	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	0	77	theme	feline	79:84	arg1	microbiota					92:101	feline fecal microbiota	79:101	feline fecal microbiota	79:101	In vitro effect of dietary protein level and nondigestible oligosaccharides on feline fecal microbiota.
25367521	18	78	theme	present	2243:2249	arg1	study					2251:2255	the present study	2239:2255	the present study	2239:2255	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	14	79	theme	amines	1837:1842	arg1	pH					1793:1794	a higher pH	1784:1794	a higher pH	1784:1794	The CTRL-HPs resulted in a higher pH and increased concentrations of biogenic amines were found after 6 and 24 h of incubation (P<0.05); putrescine at 24 h showed an increase (P<0.05) in cultures treated with FOS.
25367521	14	79	theme	amines	1837:1842	arg1	concentrations					1810:1823	increased concentrations	1800:1823	increased concentrations of biogenic amines	1800:1842	The CTRL-HPs resulted in a higher pH and increased concentrations of biogenic amines were found after 6 and 24 h of incubation (P<0.05); putrescine at 24 h showed an increase (P<0.05) in cultures treated with FOS.
25367521	13	80	dep	level	1701:1705	arg1	mmol/L					1722:1727	36.2 vs. 50.2 mmol/L	1708:1727	36.2 vs. 50.2 mmol/L for LP and HP	1708:1741	Ammonia at 24 h was affected (P<0.05) by the protein level (36.2 vs. 50.2 mmol/L for LP and HP, respectively).
25367521	11	81	with	CTRL	1377:1380	arg1	addition					1390:1397	no addition	1387:1397	no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC	1387:1562	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	18	82	theme	protein	2398:2404	arg1	levels					2406:2411	high dietary protein levels	2385:2411	high dietary protein levels in a cat's diet	2385:2427	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	18	83	dep	composition	2319:2329	arg1	the					2315:2317	the	2315:2317	the	2315:2317	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	18	84	theme	high	2385:2388	arg1	levels					2406:2411	high dietary protein levels	2385:2411	high dietary protein levels in a cat's diet	2385:2427	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	12	85	theme	fecal	1603:1607	arg1	cultures					1609:1616	feline fecal cultures	1596:1616	feline fecal cultures	1596:1616	Both FOS and LAC were added to feline fecal cultures at 2 g/L for 24 h incubation.
25367521	4	86	theme	h	631:631	arg1	incubation					633:642	24 h incubation	628:642	24 h incubation	628:642	Substrates were added to feline fecal cultures at 2 g/L for 24 h incubation.
25367521	13	87	from	h	1662:1662	arg1	Ammonia					1648:1654	Ammonia	1648:1654	Ammonia at 24 h	1648:1662	Ammonia at 24 h was affected (P<0.05) by the protein level (36.2 vs. 50.2 mmol/L for LP and HP, respectively).
25367521	7	88	theme	Putrescine	854:863	arg1	concentrations					865:878	Putrescine concentrations	854:878	Putrescine concentrations at 24 h	854:886	Putrescine concentrations at 24 h were greater (P<0.05) in cultures treated with FOS (+90%), GOS (+96%), and LAC (+87%).
25367521	13	89	theme	protein	1693:1699	arg1	level					1701:1705	the protein level	1689:1705	the protein level (36.2 vs. 50.2 mmol/L for LP and HP, respectively)	1689:1756	Ammonia at 24 h was affected (P<0.05) by the protein level (36.2 vs. 50.2 mmol/L for LP and HP, respectively).
25367521	2	90	dep	in	291:292	arg1	vitro					294:298	vitro	294:298	vitro	294:298	Two in vitro studies were conducted.
25367521	11	91	with	CTRL	1442:1445	arg1	addition					1455:1462	no addition	1452:1462	no addition of prebiotics (CTRL-HP)	1452:1486	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	11	92	theme	LP	1525:1526	arg1	diet					1528:1531	LP diet	1525:1531	LP diet	1525:1531	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	0	93	theme	protein	27:33	arg1	level					35:39	dietary protein level	19:39	dietary protein level	19:39	In vitro effect of dietary protein level and nondigestible oligosaccharides on feline fecal microbiota.
25367521	1	94	theme	feline	262:267	arg1	microbiota					275:284	feline fecal microbiota	262:284	feline fecal microbiota	262:284	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	11	95	dep	were	1341:1344	arg1	CTRL					1377:1380	low-protein (LP) CTRL	1360:1380	low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC	1360:1562	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	11	96	theme	CTRL-HP	1547:1553	arg1	addition					1390:1397	no addition	1387:1397	no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC	1387:1562	There were 6 treatments: low-protein (LP) CTRL with no addition of prebiotics (CTRL-LP), high-protein (HP) CTRL with no addition of prebiotics (CTRL-HP), LP diet plus FOS, CTRL-HP plus FOS, LP diet plus LAC, and CTRL-HP plus LAC.
25367521	16	97	theme	Lactobacillus	2170:2182	arg1	spp					2184:2186	reduced Lactobacillus spp	2162:2186	reduced Lactobacillus spp	2162:2186	At 24 h, the CTRL-HPs were associated with increased Clostridium perfringens and reduced Lactobacillus spp.
25367521	1	98	theme	microbiota	275:284	arg1	activity					250:257	activity	250:257	activity	250:257	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	1	98	theme	microbiota	275:284	arg1	composition					234:244	composition	234:244	composition	234:244	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
25367521	14	99	theme	higher	1786:1791	arg1	pH					1793:1794	a higher pH	1784:1794	a higher pH	1784:1794	The CTRL-HPs resulted in a higher pH and increased concentrations of biogenic amines were found after 6 and 24 h of incubation (P<0.05); putrescine at 24 h showed an increase (P<0.05) in cultures treated with FOS.
25367521	18	100	theme	feline	2347:2352	arg1	microbiota					2365:2374	feline intestinal microbiota	2347:2374	feline intestinal microbiota	2347:2374	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	3	101	theme	citrus	548:553	arg1	fruit					555:559	citrus fruit	548:559	citrus fruit (PEC)	548:565	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	101	theme	citrus	548:553	arg1	PEC					562:564	PEC	562:564	PEC	562:564	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	8	102	theme	total	999:1003	arg1	VFA					1005:1007	total VFA	999:1007	total VFA	999:1007	Compared with the CTRL, total VFA were higher (P<0.05) in bottles containing CF (+41%), whereas the acetic to propionic acid ratio was reduced by LAC (-51%; P<0.05).
25367521	2	103	theme	in	291:292	arg1	studies					300:306	Two in vitro studies	287:306	Two in vitro studies	287:306	Two in vitro studies were conducted.
25367521	18	104	theme	different	2294:2302	arg1	effects					2304:2310	different effects	2294:2310	different effects	2294:2310	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	18	105	theme	intestinal	2354:2363	arg1	microbiota					2365:2374	feline intestinal microbiota	2347:2374	feline intestinal microbiota	2347:2374	The results from the present study show that different prebiotics exert different effects on the composition and activity of feline intestinal microbiota and that high dietary protein levels in a cat's diet can have negative effects on the animal intestinal environment.
25367521	8	106	contain	containing	1041:1050	arg2	CF					1052:1053	CF	1052:1053	CF (+41%)	1052:1060	Compared with the CTRL, total VFA were higher (P<0.05) in bottles containing CF (+41%), whereas the acetic to propionic acid ratio was reduced by LAC (-51%; P<0.05).
25367521	8	106	contain	containing	1041:1050	arg2	%					1059:1059	+41%	1056:1059	+41%	1056:1059	Compared with the CTRL, total VFA were higher (P<0.05) in bottles containing CF (+41%), whereas the acetic to propionic acid ratio was reduced by LAC (-51%; P<0.05).
25367521	8	106	contain	containing	1041:1050	arg1	bottles					1033:1039	bottles	1033:1039	bottles containing CF (+41%)	1033:1060	Compared with the CTRL, total VFA were higher (P<0.05) in bottles containing CF (+41%), whereas the acetic to propionic acid ratio was reduced by LAC (-51%; P<0.05).
25367521	3	107	from	fruit	555:559	arg1	fiber					442:446	carrot fiber	435:446	carrot fiber (CF)	435:451	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	diet					402:405	control diet	394:405	control diet (CTRL)	394:412	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	FOS					478:480	FOS	478:480	FOS	478:480	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	CF					449:450	CF	449:450	CF	449:450	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	pectins					535:541	pectins	535:541	pectins from citrus fruit (PEC)	535:565	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	CTRL					408:411	CTRL	408:411	CTRL	408:411	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	GA					430:431	GA	430:431	GA	430:431	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	lactitol					515:522	lactitol	515:522	lactitol (LAC)	515:528	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	galactooligosaccharides					484:506	galactooligosaccharides	484:506	galactooligosaccharides (GOS)	484:512	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	LAC					525:527	LAC	525:527	LAC	525:527	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	fructooligosaccharides					454:475	fructooligosaccharides	454:475	fructooligosaccharides (FOS)	454:481	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	GOS					509:511	GOS	509:511	GOS	509:511	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	3	107	from	fruit	555:559	arg1	acid					424:427	gluconic acid	415:427	gluconic acid (GA)	415:432	First, 6 nondigestible oligosaccharides were studied; treatments were control diet (CTRL), gluconic acid (GA), carrot fiber (CF), fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactitol (LAC), and pectins from citrus fruit (PEC).
25367521	5	108	dep	reduced	686:692	arg1	P<0.05					695:700	P<0.05	695:700	P<0.05	695:700	Compared with the CTRL, ammonia had been reduced (P<0.05) by GOS (-9%) after 6 h and by GA (-14%), LAC (-12%), and PEC (-10%) after 24 h.
25367521	5	108	dep	reduced	686:692	arg1	%					713:713	-9%	711:713	-9%	711:713	Compared with the CTRL, ammonia had been reduced (P<0.05) by GOS (-9%) after 6 h and by GA (-14%), LAC (-12%), and PEC (-10%) after 24 h.
25367521	1	109	theme	protein	212:218	arg1	levels					220:225	2 dietary protein levels	202:225	2 dietary protein levels	202:225	The aim of the present study was to evaluate in vitro the effect of some prebiotic substances and 2 dietary protein levels on the composition and activity of feline fecal microbiota.
26463048	1	0	theme	acid	294:297	arg1	hydrogel					304:311	a hyaluronic acid (HA) hydrogel	281:311	a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation	281:358	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	3	1	theme	spinal	803:808	arg1	repair					810:815	spinal repair	803:815	spinal repair	803:815	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	0	2	theme	scaffold	72:79	arg1	implantation					29:40	implantation	29:40	implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat	29:113	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.
26463048	6	3	theme	neural	1203:1208	arg1	regeneration					1210:1221	neural regeneration	1203:1221	neural regeneration following SCI	1203:1235	Thus, this novel composite material might provide a suitable microenvironment for neural regeneration following SCI.
26463048	1	4	theme	optimal	138:144	arg1	microenvironment					146:161	an optimal microenvironment	135:161	an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI)	135:235	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	4	5	theme	vessels	967:973	arg1	numbers					946:952	large numbers	940:952	large numbers of new blood vessels and regenerated nerve fibers	940:1002	In particular, large numbers of new blood vessels and regenerated nerve fibers were found within and around the implants.
26463048	2	6	theme	neurotrophic	542:553	arg1	factor					555:560	brain-derived neurotrophic factor	528:560	brain-derived neurotrophic factor	528:560	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	4	7	theme	blood	961:965	arg1	vessels					967:973	new blood vessels	957:973	new blood vessels	957:973	In particular, large numbers of new blood vessels and regenerated nerve fibers were found within and around the implants.
26463048	0	8	theme	PLGA	86:89	arg1	microspheres					91:102	PLGA microspheres	86:102	PLGA microspheres in the rat	86:113	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.
26463048	2	9	theme	brain-derived	528:540	arg1	factor					555:560	brain-derived neurotrophic factor	528:560	brain-derived neurotrophic factor	528:560	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	3	10	theme	favorable	743:751	arg1	effects					753:759	favorable effects	743:759	favorable effects	743:759	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	3	11	theme	injured	669:675	arg1	area					677:680	an injured area	666:680	an injured area created by a dorsal hemisection at T9-10 of the spinal cord	666:740	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	6	12	theme	suitable	1173:1180	arg1	microenvironment					1182:1197	a suitable microenvironment	1171:1197	a suitable microenvironment for neural regeneration following SCI	1171:1235	Thus, this novel composite material might provide a suitable microenvironment for neural regeneration following SCI.
26463048	3	13	theme	excellent	828:836	arg1	integration					838:848	excellent integration	828:848	excellent integration of the implants with host tissue	828:881	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	3	14	theme	composite	651:659	arg1	implantation					630:641	implantation	630:641	implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord	630:740	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	2	15	theme	growth	587:592	arg1	HA+PLGA					602:608	HA+PLGA	602:608	HA+PLGA	602:608	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	2	15	theme	growth	587:592	arg1	factor					594:599	vascular endothelial growth factor	566:599	vascular endothelial growth factor (HA+PLGA)	566:609	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	3	16	theme	cord	737:740	arg1	T9-10					717:721	T9-10	717:721	T9-10 of the spinal cord	717:740	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	3	17	theme	repair	810:815	arg1	promotion					790:798	the promotion	786:798	the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis	786:922	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	5	18	theme	locomotor	1101:1109	arg1	recovery					1111:1118	improved locomotor recovery	1092:1118	improved locomotor recovery	1092:1118	Simultaneously, the implanted rats exhibited improved locomotor recovery.
26463048	2	19	theme	endothelial	575:585	arg1	HA+PLGA					602:608	HA+PLGA	602:608	HA+PLGA	602:608	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	2	19	theme	endothelial	575:585	arg1	factor					594:599	vascular endothelial growth factor	566:599	vascular endothelial growth factor (HA+PLGA)	566:609	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	1	20	theme	neural	167:172	arg1	regeneration					174:185	neural regeneration	167:185	neural regeneration in the lesion area after spinal cord injury (SCI)	167:235	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	0	21	theme	Spinal	0:5	arg1	cord					7:10	Spinal cord injury repair	0:24	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.	0:114	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.
26463048	2	22	theme	vascular	566:573	arg1	HA+PLGA					602:608	HA+PLGA	602:608	HA+PLGA	602:608	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	2	22	theme	vascular	566:573	arg1	factor					594:599	vascular endothelial growth factor	566:599	vascular endothelial growth factor (HA+PLGA)	566:609	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	4	23	theme	nerve	991:995	arg1	fibers					997:1002	regenerated nerve fibers	979:1002	regenerated nerve fibers	979:1002	In particular, large numbers of new blood vessels and regenerated nerve fibers were found within and around the implants.
26463048	3	24	theme	implants	857:864	arg1	inhibition					884:893	inhibition	884:893	inhibition of inflammation	884:909	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	3	24	theme	implants	857:864	arg1	gliosis					916:922	gliosis	916:922	gliosis	916:922	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	3	24	theme	implants	857:864	arg1	integration					838:848	excellent integration	828:848	excellent integration of the implants with host tissue	828:881	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	0	25	theme	injury	12:17	arg1	repair					19:24	injury repair	12:24	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.	0:114	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.
26463048	5	26	theme	improved	1092:1099	arg1	recovery					1111:1118	improved locomotor recovery	1092:1118	improved locomotor recovery	1092:1118	Simultaneously, the implanted rats exhibited improved locomotor recovery.
26463048	1	27	theme	longitudinal	320:331	arg1	conformation					347:358	a longitudinal multi-tubular conformation	318:358	a longitudinal multi-tubular conformation	318:358	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	2	28	theme	receptor	416:423	arg1	antiNgR					435:441	antiNgR	435:441	antiNgR	435:441	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	2	28	theme	receptor	416:423	arg1	antibody					425:432	an anti-Nogo receptor antibody	403:432	an anti-Nogo receptor antibody (antiNgR)	403:442	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	1	29	theme	novel	254:258	arg1	scaffold					260:267	a novel scaffold	252:267	a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation	252:358	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	2	30	theme	acid	491:494	arg1	microspheres					504:515	poly(lactic-co-glycolic acid) (PLGA) microspheres	467:515	poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA)	467:609	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	1	31	theme	multi-tubular	333:345	arg1	conformation					347:358	a longitudinal multi-tubular conformation	318:358	a longitudinal multi-tubular conformation	318:358	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	2	32	contain	containing	517:526	arg2	factor					594:599	vascular endothelial growth factor	566:599	vascular endothelial growth factor (HA+PLGA)	566:609	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	2	32	contain	containing	517:526	arg1	microspheres					504:515	poly(lactic-co-glycolic acid) (PLGA) microspheres	467:515	poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA)	467:609	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	2	32	contain	containing	517:526	arg2	HA+PLGA					602:608	HA+PLGA	602:608	HA+PLGA	602:608	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	2	32	contain	containing	517:526	arg2	factor					555:560	brain-derived neurotrophic factor	528:560	brain-derived neurotrophic factor	528:560	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	1	33	theme	lesion	194:199	arg1	area					201:204	the lesion area	190:204	the lesion area	190:204	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	2	34	theme	lactic-co-glycolic	472:489	arg1	microspheres					504:515	poly(lactic-co-glycolic acid) (PLGA) microspheres	467:515	poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA)	467:609	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	6	35	theme	composite	1138:1146	arg1	material					1148:1155	this novel composite material	1127:1155	this novel composite material	1127:1155	Thus, this novel composite material might provide a suitable microenvironment for neural regeneration following SCI.
26463048	0	36	theme	structured	45:54	arg1	acid					67:70	structured hyaluronic acid	45:70	structured hyaluronic acid scaffold	45:79	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.
26463048	3	37	with	implants	857:864	arg1	tissue					876:881	host tissue	871:881	host tissue	871:881	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	6	38	theme	novel	1132:1136	arg1	material					1148:1155	this novel composite material	1127:1155	this novel composite material	1127:1155	Thus, this novel composite material might provide a suitable microenvironment for neural regeneration following SCI.
26463048	2	39	theme	anti-Nogo	406:414	arg1	antiNgR					435:441	antiNgR	435:441	antiNgR	435:441	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	2	39	theme	anti-Nogo	406:414	arg1	antibody					425:432	an anti-Nogo receptor antibody	403:432	an anti-Nogo receptor antibody (antiNgR)	403:442	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	3	40	theme	dorsal	695:700	arg1	hemisection					702:712	a dorsal hemisection	693:712	a dorsal hemisection	693:712	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	4	41	located	found	1009:1013	arg2	numbers					946:952	large numbers	940:952	large numbers of new blood vessels and regenerated nerve fibers	940:1002	In particular, large numbers of new blood vessels and regenerated nerve fibers were found within and around the implants.
26463048	4	41	located	found	1009:1013	arg1	particular					928:937	particular	928:937	particular	928:937	In particular, large numbers of new blood vessels and regenerated nerve fibers were found within and around the implants.
26463048	4	42	theme	regenerated	979:989	arg1	fibers					997:1002	regenerated nerve fibers	979:1002	regenerated nerve fibers	979:1002	In particular, large numbers of new blood vessels and regenerated nerve fibers were found within and around the implants.
26463048	5	43	theme	implanted	1067:1075	arg1	rats					1077:1080	the implanted rats	1063:1080	the implanted rats	1063:1080	Simultaneously, the implanted rats exhibited improved locomotor recovery.
26463048	3	44	theme	host	871:874	arg1	tissue					876:881	host tissue	871:881	host tissue	871:881	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	2	45	link	brain-derived	528:540	arg1	factor					555:560	brain-derived neurotrophic factor	528:560	brain-derived neurotrophic factor	528:560	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	3	46	theme	spinal	730:735	arg1	cord					737:740	the spinal cord	726:740	the spinal cord	726:740	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	2	47	theme	PLGA	498:501	arg1	microspheres					504:515	poly(lactic-co-glycolic acid) (PLGA) microspheres	467:515	poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA)	467:609	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
26463048	0	48	from	microspheres	91:102	arg1	rat					111:113	the rat	107:113	the rat	107:113	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.
26463048	3	49	theme	inflammation	898:909	arg1	inhibition					884:893	inhibition	884:893	inhibition of inflammation	884:909	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	3	49	theme	inflammation	898:909	arg1	gliosis					916:922	gliosis	916:922	gliosis	916:922	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	3	49	theme	inflammation	898:909	arg1	integration					838:848	excellent integration	828:848	excellent integration of the implants with host tissue	828:881	In the rat, after implantation of this composite into an injured area created by a dorsal hemisection at T9-10 of the spinal cord, favorable effects were seen with regard to the promotion of spinal repair, including excellent integration of the implants with host tissue, inhibition of inflammation, and gliosis.
26463048	4	50	theme	fibers	997:1002	arg1	numbers					946:952	large numbers	940:952	large numbers of new blood vessels and regenerated nerve fibers	940:1002	In particular, large numbers of new blood vessels and regenerated nerve fibers were found within and around the implants.
26463048	1	51	theme	spinal	212:217	arg1	SCI					232:234	SCI	232:234	SCI	232:234	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	1	51	theme	spinal	212:217	arg1	injury					224:229	spinal cord injury	212:229	spinal cord injury (SCI)	212:235	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	0	52	theme	acid	67:70	arg1	scaffold					72:79	structured hyaluronic acid scaffold	45:79	structured hyaluronic acid scaffold	45:79	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.
26463048	0	53	dep	cord	7:10	arg1	repair					19:24	injury repair	12:24	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.	0:114	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.
26463048	1	54	from	regeneration	174:185	arg1	area					201:204	the lesion area	190:204	the lesion area	190:204	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	1	55	with	hydrogel	304:311	arg1	conformation					347:358	a longitudinal multi-tubular conformation	318:358	a longitudinal multi-tubular conformation	318:358	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	1	56	theme	cord	219:222	arg1	SCI					232:234	SCI	232:234	SCI	232:234	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	1	56	theme	cord	219:222	arg1	injury					224:229	spinal cord injury	212:229	spinal cord injury (SCI)	212:235	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	0	57	theme	hyaluronic	56:65	arg1	acid					67:70	structured hyaluronic acid	45:70	structured hyaluronic acid scaffold	45:79	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.
26463048	1	58	theme	hyaluronic	283:292	arg1	HA					300:301	HA	300:301	HA	300:301	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	1	58	theme	hyaluronic	283:292	arg1	acid					294:297	hyaluronic acid	283:297	a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation	281:358	In order to create an optimal microenvironment for neural regeneration in the lesion area after spinal cord injury (SCI), we fabricated a novel scaffold composed of a hyaluronic acid (HA) hydrogel with a longitudinal multi-tubular conformation.
26463048	4	59	theme	large	940:944	arg1	numbers					946:952	large numbers	940:952	large numbers of new blood vessels and regenerated nerve fibers	940:1002	In particular, large numbers of new blood vessels and regenerated nerve fibers were found within and around the implants.
26463048	0	60	with	implantation	29:40	arg1	microspheres					91:102	PLGA microspheres	86:102	PLGA microspheres in the rat	86:113	Spinal cord injury repair by implantation of structured hyaluronic acid scaffold with PLGA microspheres in the rat.
26463048	4	61	theme	new	957:959	arg1	vessels					967:973	new blood vessels	957:973	new blood vessels	957:973	In particular, large numbers of new blood vessels and regenerated nerve fibers were found within and around the implants.
26463048	2	62	theme	poly	467:470	arg1	microspheres					504:515	poly(lactic-co-glycolic acid) (PLGA) microspheres	467:515	poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA)	467:609	The scaffold was modified by binding with an anti-Nogo receptor antibody (antiNgR) and mixed further with poly(lactic-co-glycolic acid) (PLGA) microspheres containing brain-derived neurotrophic factor and vascular endothelial growth factor (HA+PLGA).
27774742	2	0	theme	second	600:605	arg1	dimension					607:615	the second dimension	596:615	the second dimension	596:615	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	2	1	from	column	530:535	arg1	column					586:591	the first dimension and α polyethylene glycol column	540:591	the first dimension and α polyethylene glycol column in the second dimension	540:615	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	2	2	theme	column	381:386	arg1	series					388:393	Two capillary column series	367:393	Two capillary column series	367:393	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	2	2	theme	column	381:386	arg1	column					530:535	the chiral column	519:535	the chiral column in the first dimension and α polyethylene glycol column in the second dimension	519:615	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	2	3	used	used	400:403	arg2	column					530:535	the chiral column	519:535	the chiral column in the first dimension and α polyethylene glycol column in the second dimension	519:615	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	2	3	used	used	400:403	arg2	series					388:393	Two capillary column series	367:393	Two capillary column series	367:393	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	1	4	theme	hyphenated	291:300	arg1	detection					356:364	hyphenated to flame ionization and quadrupole mass spectrometric detection	291:364	hyphenated to flame ionization and quadrupole mass spectrometric detection	291:364	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	2	5	theme	capillary	371:379	arg1	series					388:393	Two capillary column series	367:393	Two capillary column series	367:393	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	2	5	theme	capillary	371:379	arg1	column					530:535	the chiral column	519:535	the chiral column in the first dimension and α polyethylene glycol column in the second dimension	519:615	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	4	6	theme	lavender	868:875	arg1	oil					887:889	lavender essential oil	868:889	lavender essential oil	868:889	This maybe a possible route to assess the authenticity of lavender essential oil.
27774742	1	7	from	oil	170:172	arg1	composition					110:120	The enantiomeric composition	93:120	The enantiomeric composition of several chiral markers in lavender essential oil	93:172	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	3	8	theme	column	671:676	arg1	series					678:683	these column series	665:683	these column series	665:683	Combining the chromatographic data obtained on these column series, the enantiomeric and excess ratios for α-pinene, β-pinene, camphor, lavandulol, borneol, and terpinen-4-ol were determined.
27774742	1	9	theme	flame	305:309	arg1	ionization					311:320	flame ionization	305:320	flame ionization	305:320	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	4	10	theme	oil	887:889	arg1	authenticity					852:863	the authenticity	848:863	the authenticity of lavender essential oil	848:889	This maybe a possible route to assess the authenticity of lavender essential oil.
27774742	1	11	theme	flow	189:192	arg1	chromatography					238:251	flow modulated comprehensive two-dimensional gas chromatography	189:251	flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection	189:364	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	2	12	theme	dimension and α polyethylene	550:577	arg1	column					586:591	the first dimension and α polyethylene glycol column	540:591	the first dimension and α polyethylene glycol column in the second dimension	540:615	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	1	13	theme	enantiomeric	97:108	arg1	composition					110:120	The enantiomeric composition	93:120	The enantiomeric composition of several chiral markers in lavender essential oil	93:172	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	1	14	theme	modulated	194:202	arg1	chromatography					238:251	flow modulated comprehensive two-dimensional gas chromatography	189:251	flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection	189:364	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	0	15	theme	comprehensive	17:29	arg1	chromatography					51:64	Enantioselective comprehensive two-dimensional gas chromatography	0:64	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil	0:90	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil.
27774742	2	16	from	column	586:591	arg1	dimension					607:615	the second dimension	596:615	the second dimension	596:615	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	1	17	theme	comprehensive	204:216	arg1	chromatography					238:251	flow modulated comprehensive two-dimensional gas chromatography	189:251	flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection	189:364	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	0	18	theme	Enantioselective	0:15	arg1	chromatography					51:64	Enantioselective comprehensive two-dimensional gas chromatography	0:64	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil	0:90	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil.
27774742	4	19	theme	possible	823:830	arg1	route					832:836	a possible route	821:836	a possible route	821:836	This maybe a possible route to assess the authenticity of lavender essential oil.
27774742	1	20	from	markers	140:146	arg1	oil					170:172	lavender essential oil	151:172	lavender essential oil	151:172	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	3	21	theme	enantiomeric	690:701	arg1	ratios					714:719	the enantiomeric and excess ratios	686:719	the enantiomeric and excess ratios for α-pinene, β-pinene, camphor, lavandulol, borneol, and terpinen-4-ol	686:791	Combining the chromatographic data obtained on these column series, the enantiomeric and excess ratios for α-pinene, β-pinene, camphor, lavandulol, borneol, and terpinen-4-ol were determined.
27774742	1	22	theme	two-dimensional	218:232	arg1	chromatography					238:251	flow modulated comprehensive two-dimensional gas chromatography	189:251	flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection	189:364	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	0	23	theme	gas	47:49	arg1	chromatography					51:64	Enantioselective comprehensive two-dimensional gas chromatography	0:64	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil	0:90	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil.
27774742	1	24	theme	several	125:131	arg1	markers					140:146	several chiral markers	125:146	several chiral markers in lavender essential oil	125:172	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	1	25	theme	gas	234:236	arg1	chromatography					238:251	flow modulated comprehensive two-dimensional gas chromatography	189:251	flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection	189:364	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	0	26	theme	two-dimensional	31:45	arg1	chromatography					51:64	Enantioselective comprehensive two-dimensional gas chromatography	0:64	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil	0:90	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil.
27774742	1	27	theme	chiral	133:138	arg1	markers					140:146	several chiral markers	125:146	several chiral markers in lavender essential oil	125:172	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	2	28	theme	glycol	579:584	arg1	column					586:591	the first dimension and α polyethylene glycol column	540:591	the first dimension and α polyethylene glycol column in the second dimension	540:615	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	1	29	theme	spectrometric	342:354	arg1	detection					356:364	hyphenated to flame ionization and quadrupole mass spectrometric detection	291:364	hyphenated to flame ionization and quadrupole mass spectrometric detection	291:364	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	0	30	theme	essential	78:86	arg1	oil					88:90	lavender essential oil	69:90	lavender essential oil	69:90	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil.
27774742	1	31	theme	markers	140:146	arg1	composition					110:120	The enantiomeric composition	93:120	The enantiomeric composition of several chiral markers in lavender essential oil	93:172	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	0	32	theme	lavender	69:76	arg1	oil					88:90	lavender essential oil	69:90	lavender essential oil	69:90	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil.
27774742	3	33	theme	excess	707:712	arg1	ratios					714:719	the enantiomeric and excess ratios	686:719	the enantiomeric and excess ratios for α-pinene, β-pinene, camphor, lavandulol, borneol, and terpinen-4-ol	686:791	Combining the chromatographic data obtained on these column series, the enantiomeric and excess ratios for α-pinene, β-pinene, camphor, lavandulol, borneol, and terpinen-4-ol were determined.
27774742	4	34	dep	assess	841:846	arg1	maybe					815:819	This maybe	810:819	This maybe	810:819	This maybe a possible route to assess the authenticity of lavender essential oil.
27774742	1	35	theme	lavender	151:158	arg1	oil					170:172	lavender essential oil	151:172	lavender essential oil	151:172	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	4	36	theme	essential	877:885	arg1	oil					887:889	lavender essential oil	868:889	lavender essential oil	868:889	This maybe a possible route to assess the authenticity of lavender essential oil.
27774742	1	37	theme	essential	160:168	arg1	oil					170:172	lavender essential oil	151:172	lavender essential oil	151:172	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	1	38	from	composition	110:120	arg1	oil					170:172	lavender essential oil	151:172	lavender essential oil	151:172	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	1	39	theme	reverse	269:275	arg1	mode					282:285	the reverse flow mode	265:285	the reverse flow mode	265:285	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	2	40	theme	chiral	523:528	arg1	series					388:393	Two capillary column series	367:393	Two capillary column series	367:393	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	2	40	theme	chiral	523:528	arg1	column					530:535	the chiral column	519:535	the chiral column in the first dimension and α polyethylene glycol column in the second dimension	519:615	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
27774742	3	41	theme	chromatographic	632:646	arg1	data					648:651	the chromatographic data	628:651	the chromatographic data obtained on these column series	628:683	Combining the chromatographic data obtained on these column series, the enantiomeric and excess ratios for α-pinene, β-pinene, camphor, lavandulol, borneol, and terpinen-4-ol were determined.
27774742	1	42	theme	flow	277:280	arg1	mode					282:285	the reverse flow mode	265:285	the reverse flow mode	265:285	The enantiomeric composition of several chiral markers in lavender essential oil was studied by flow modulated comprehensive two-dimensional gas chromatography operated in the reverse flow mode and hyphenated to flame ionization and quadrupole mass spectrometric detection.
27774742	0	43	theme	oil	88:90	arg1	chromatography					51:64	Enantioselective comprehensive two-dimensional gas chromatography	0:64	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil	0:90	Enantioselective comprehensive two-dimensional gas chromatography of lavender essential oil.
27774742	2	44	theme	first	544:548	arg1	column					586:591	the first dimension and α polyethylene glycol column	540:591	the first dimension and α polyethylene glycol column in the second dimension	540:615	Two capillary column series were used in this study, 2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl-β-cyclodextrin or 2,3,6-tri-O-methyl-β-cyclodextrin, as the chiral column in the first dimension and α polyethylene glycol column in the second dimension.
28655159	8	0	theme	Short-term	1164:1173	arg1	nutrition					1193:1201	Short-term exclusive enteral nutrition	1164:1201	Short-term exclusive enteral nutrition	1164:1201	Short-term exclusive enteral nutrition elicited limited impact on the overall composition of the CD microbiota, although functional changes occurred following treatment.
28655159	7	1	from	evident	1083:1089	arg1	CD					1094:1095	CD	1094:1095	CD	1094:1095	Disruption of ecological networks was evident in CD, coupled with reduction in growth rates of many bacterial species.
28655159	7	2	theme	many	1140:1143	arg1	species					1155:1161	many bacterial species	1140:1161	many bacterial species	1140:1161	Disruption of ecological networks was evident in CD, coupled with reduction in growth rates of many bacterial species.
28655159	8	3	theme	enteral	1185:1191	arg1	nutrition					1193:1201	Short-term exclusive enteral nutrition	1164:1201	Short-term exclusive enteral nutrition	1164:1201	Short-term exclusive enteral nutrition elicited limited impact on the overall composition of the CD microbiota, although functional changes occurred following treatment.
28655159	7	4	from	CD	1094:1095	arg1	evident					1083:1089	evident	1083:1089	evident	1083:1089	Disruption of ecological networks was evident in CD, coupled with reduction in growth rates of many bacterial species.
28655159	8	5	theme	microbiota	1264:1273	arg1	composition					1242:1252	the overall composition	1230:1252	the overall composition of the CD microbiota	1230:1273	Short-term exclusive enteral nutrition elicited limited impact on the overall composition of the CD microbiota, although functional changes occurred following treatment.
28655159	6	6	from	enrichment	884:893	arg1	producers					898:906	producers	898:906	producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids	898:1042	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	1	7	theme	challenge	168:176	arg1	worldwide					178:186	a health challenge worldwide	159:186	a health challenge worldwide	159:186	The inflammatory intestinal disorder Crohn's disease (CD) has become a health challenge worldwide.
28655159	6	8	from	shifts	864:869	arg1	CD					874:875	CD	874:875	CD	874:875	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	3	9	from	studies	310:316	arg1	number					329:334	a small number	321:334	a small number of CD patients	321:349	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	5	10	theme	microbiome	808:817	arg1	structure					819:827	CD microbiome structure	805:827	CD microbiome structure	805:827	Based on signature taxa, CD microbiotas clustered into 2 distinct metacommunities, indicating individual variability in CD microbiome structure.
28655159	4	11	theme	unannotated	599:609	arg1	bacteria					611:618	also unannotated bacteria	594:618	also unannotated bacteria	594:618	Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition.
28655159	9	12	theme	healthy	1535:1541	arg1	individuals					1543:1553	the healthy individuals	1531:1553	the healthy individuals	1531:1553	The microbiotas in CD patients can be stratified into 2 distinct metacommunities, with the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis.
28655159	6	13	theme	variant	965:971	arg1	producers					898:906	producers	898:906	producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids	898:1042	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	6	14	theme	hexa-acylated	932:944	arg1	variant					965:971	the pro-inflammatory hexa-acylated lipopolysaccharide variant	911:971	the pro-inflammatory hexa-acylated lipopolysaccharide variant	911:971	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	7	15	theme	ecological	1059:1068	arg1	networks					1070:1077	ecological networks	1059:1077	ecological networks	1059:1077	Disruption of ecological networks was evident in CD, coupled with reduction in growth rates of many bacterial species.
28655159	2	16	theme	immune	240:245	arg1	system					247:252	the host immune system	231:252	the host immune system	231:252	The gut microbiota closely interacts with the host immune system, but its functional impact in CD is unclear.
28655159	9	17	theme	possible	1561:1568	arg1	implication					1570:1580	possible implication	1561:1580	possible implication in relation to CD pathogenesis	1561:1611	The microbiotas in CD patients can be stratified into 2 distinct metacommunities, with the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis.
28655159	4	18	theme	features	552:559	arg1	characterization					499:514	a detailed characterization	488:514	a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria	488:618	Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition.
28655159	6	19	theme	fatty	1032:1036	arg1	acids					1038:1042	short-chain fatty acids	1020:1042	short-chain fatty acids	1020:1042	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	4	20	theme	exclusive	656:664	arg1	nutrition					674:682	exclusive enteral nutrition	656:682	exclusive enteral nutrition	656:682	Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition.
28655159	0	21	theme	disease	72:78	arg1	patients					80:87	Crohn's disease patients	64:87	Crohn's disease patients	64:87	Two distinct metacommunities characterize the gut microbiota in Crohn's disease patients.
28655159	4	22	theme	metagenomic	446:456	arg1	sequencing					466:475	metagenomic shotgun sequencing	446:475	metagenomic shotgun sequencing	446:475	Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition.
28655159	5	23	theme	signature	694:702	arg1	taxa					704:707	signature taxa	694:707	signature taxa	694:707	Based on signature taxa, CD microbiotas clustered into 2 distinct metacommunities, indicating individual variability in CD microbiome structure.
28655159	5	24	from	variability	790:800	arg1	structure					819:827	CD microbiome structure	805:827	CD microbiome structure	805:827	Based on signature taxa, CD microbiotas clustered into 2 distinct metacommunities, indicating individual variability in CD microbiome structure.
28655159	0	25	from	microbiota	50:59	arg1	patients					80:87	Crohn's disease patients	64:87	Crohn's disease patients	64:87	Two distinct metacommunities characterize the gut microbiota in Crohn's disease patients.
28655159	1	26	dep	disorder	118:125	arg1	CD					144:145	CD	144:145	CD	144:145	The inflammatory intestinal disorder Crohn's disease (CD) has become a health challenge worldwide.
28655159	1	26	dep	disorder	118:125	arg1	disease					135:141	Crohn's disease	127:141	The inflammatory intestinal disorder Crohn's disease (CD)	90:146	The inflammatory intestinal disorder Crohn's disease (CD) has become a health challenge worldwide.
28655159	3	27	theme	CD	339:340	arg1	patients					342:349	CD patients	339:349	CD patients	339:349	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	3	28	used	used	394:397	arg2	analyses					352:359	analyses	352:359	analyses of the gut microbiota in CD	352:387	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	3	29	theme	gut	368:370	arg1	microbiota					372:381	the gut microbiota	364:381	the gut microbiota in CD	364:387	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	5	30	theme	individual	779:788	arg1	variability					790:800	individual variability	779:800	individual variability in CD microbiome structure	779:827	Based on signature taxa, CD microbiotas clustered into 2 distinct metacommunities, indicating individual variability in CD microbiome structure.
28655159	4	31	theme	detailed	490:497	arg1	characterization					499:514	a detailed characterization	488:514	a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria	488:618	Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition.
28655159	6	32	from	reduction	979:987	arg1	potential					996:1004	the potential to synthesize short-chain fatty acids	992:1042	the potential to synthesize short-chain fatty acids	992:1042	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	3	33	theme	16S	399:401	arg1	sequencing					417:426	16S rDNA amplicon sequencing	399:426	16S rDNA amplicon sequencing	399:426	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	1	34	theme	intestinal	107:116	arg1	disorder					118:125	The inflammatory intestinal disorder Crohn's disease (CD)	90:146	The inflammatory intestinal disorder Crohn's disease (CD)	90:146	The inflammatory intestinal disorder Crohn's disease (CD) has become a health challenge worldwide.
28655159	5	35	theme	distinct	742:749	arg1	metacommunities					751:765	2 distinct metacommunities	740:765	2 distinct metacommunities	740:765	Based on signature taxa, CD microbiotas clustered into 2 distinct metacommunities, indicating individual variability in CD microbiome structure.
28655159	9	36	theme	distinct	1390:1397	arg1	metacommunities					1399:1413	2 distinct metacommunities	1388:1413	2 distinct metacommunities	1388:1413	The microbiotas in CD patients can be stratified into 2 distinct metacommunities, with the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis.
28655159	6	37	theme	functional	853:862	arg1	shifts					864:869	Metacommunity-specific functional shifts	830:869	Metacommunity-specific functional shifts in CD	830:875	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	7	38	theme	species	1155:1161	arg1	rates					1131:1135	growth rates	1124:1135	growth rates of many bacterial species	1124:1161	Disruption of ecological networks was evident in CD, coupled with reduction in growth rates of many bacterial species.
28655159	3	39	theme	amplicon	408:415	arg1	sequencing					417:426	16S rDNA amplicon sequencing	399:426	16S rDNA amplicon sequencing	399:426	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	3	40	from	microbiota	372:381	arg1	CD					386:387	CD	386:387	CD	386:387	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	6	41	theme	pro-inflammatory	915:930	arg1	variant					965:971	the pro-inflammatory hexa-acylated lipopolysaccharide variant	911:971	the pro-inflammatory hexa-acylated lipopolysaccharide variant	911:971	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	4	42	theme	CD	568:569	arg1	microbiota					571:580	the CD microbiota	564:580	the CD microbiota	564:580	Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition.
28655159	3	43	from	CD	386:387	arg1	analyses					352:359	analyses	352:359	analyses of the gut microbiota in CD	352:387	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	4	44	theme	compositional	523:535	arg1	features					552:559	the compositional and functional features	519:559	the compositional and functional features	519:559	Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition.
28655159	7	45	theme	growth	1124:1129	arg1	rates					1131:1135	growth rates	1124:1135	growth rates of many bacterial species	1124:1161	Disruption of ecological networks was evident in CD, coupled with reduction in growth rates of many bacterial species.
28655159	2	46	theme	functional	263:272	arg1	impact					274:279	its functional impact	259:279	its functional impact in CD	259:285	The gut microbiota closely interacts with the host immune system, but its functional impact in CD is unclear.
28655159	9	47	theme	functional	1474:1483	arg1	potentials					1485:1494	functional potentials	1474:1494	functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis	1474:1611	The microbiotas in CD patients can be stratified into 2 distinct metacommunities, with the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis.
28655159	8	48	theme	exclusive	1175:1183	arg1	nutrition					1193:1201	Short-term exclusive enteral nutrition	1164:1201	Short-term exclusive enteral nutrition	1164:1201	Short-term exclusive enteral nutrition elicited limited impact on the overall composition of the CD microbiota, although functional changes occurred following treatment.
28655159	9	49	from	implication	1570:1580	arg1	relation					1585:1592	relation	1585:1592	relation to CD pathogenesis	1585:1611	The microbiotas in CD patients can be stratified into 2 distinct metacommunities, with the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis.
28655159	4	50	theme	functional	541:550	arg1	features					552:559	the compositional and functional features	519:559	the compositional and functional features	519:559	Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition.
28655159	9	51	with	metacommunities	1399:1413	arg1	metacommunity					1449:1461	the most severely perturbed metacommunity	1421:1461	the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis	1421:1611	The microbiotas in CD patients can be stratified into 2 distinct metacommunities, with the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis.
28655159	8	52	theme	CD	1261:1262	arg1	microbiota					1264:1273	the CD microbiota	1257:1273	the CD microbiota	1257:1273	Short-term exclusive enteral nutrition elicited limited impact on the overall composition of the CD microbiota, although functional changes occurred following treatment.
28655159	1	53	theme	health	161:166	arg1	worldwide					178:186	a health challenge worldwide	159:186	a health challenge worldwide	159:186	The inflammatory intestinal disorder Crohn's disease (CD) has become a health challenge worldwide.
28655159	8	54	theme	limited	1212:1218	arg1	impact					1220:1225	limited impact	1212:1225	limited impact	1212:1225	Short-term exclusive enteral nutrition elicited limited impact on the overall composition of the CD microbiota, although functional changes occurred following treatment.
28655159	5	55	theme	CD	805:806	arg1	structure					819:827	CD microbiome structure	805:827	CD microbiome structure	805:827	Based on signature taxa, CD microbiotas clustered into 2 distinct metacommunities, indicating individual variability in CD microbiome structure.
28655159	6	56	theme	reduction	979:987	arg1	producers					898:906	producers	898:906	producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids	898:1042	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	8	57	theme	functional	1285:1294	arg1	changes					1296:1302	functional changes	1285:1302	functional changes	1285:1302	Short-term exclusive enteral nutrition elicited limited impact on the overall composition of the CD microbiota, although functional changes occurred following treatment.
28655159	9	58	from	microbiotas	1338:1348	arg1	patients					1356:1363	CD patients	1353:1363	CD patients	1353:1363	The microbiotas in CD patients can be stratified into 2 distinct metacommunities, with the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis.
28655159	7	59	theme	networks	1070:1077	arg1	Disruption					1045:1054	Disruption	1045:1054	Disruption of ecological networks	1045:1077	Disruption of ecological networks was evident in CD, coupled with reduction in growth rates of many bacterial species.
28655159	6	60	theme	lipopolysaccharide	946:963	arg1	variant					965:971	the pro-inflammatory hexa-acylated lipopolysaccharide variant	911:971	the pro-inflammatory hexa-acylated lipopolysaccharide variant	911:971	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	4	61	theme	enteral	666:672	arg1	nutrition					674:682	exclusive enteral nutrition	656:682	exclusive enteral nutrition	656:682	Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition.
28655159	5	62	theme	CD	710:711	arg1	microbiotas					713:723	CD microbiotas	710:723	CD microbiotas	710:723	Based on signature taxa, CD microbiotas clustered into 2 distinct metacommunities, indicating individual variability in CD microbiome structure.
28655159	2	63	theme	host	235:238	arg1	system					247:252	the host immune system	231:252	the host immune system	231:252	The gut microbiota closely interacts with the host immune system, but its functional impact in CD is unclear.
28655159	3	64	from	analyses	352:359	arg1	CD					386:387	CD	386:387	CD	386:387	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	2	65	from	impact	274:279	arg1	CD					284:285	CD	284:285	CD	284:285	The gut microbiota closely interacts with the host immune system, but its functional impact in CD is unclear.
28655159	6	66	theme	short-chain	1020:1030	arg1	acids					1038:1042	short-chain fatty acids	1020:1042	short-chain fatty acids	1020:1042	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	4	67	theme	shotgun	458:464	arg1	sequencing					466:475	metagenomic shotgun sequencing	446:475	metagenomic shotgun sequencing	446:475	Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition.
28655159	9	68	theme	CD	1597:1598	arg1	pathogenesis					1600:1611	CD pathogenesis	1597:1611	CD pathogenesis	1597:1611	The microbiotas in CD patients can be stratified into 2 distinct metacommunities, with the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis.
28655159	3	69	theme	small	323:327	arg1	number					329:334	a small number	321:334	a small number of CD patients	321:349	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	9	70	theme	CD	1353:1354	arg1	patients					1356:1363	CD patients	1353:1363	CD patients	1353:1363	The microbiotas in CD patients can be stratified into 2 distinct metacommunities, with the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis.
28655159	6	71	from	potential	996:1004	arg1	producers					898:906	producers	898:906	producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids	898:1042	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	0	72	theme	distinct	4:11	arg1	metacommunities					13:27	Two distinct metacommunities	0:27	Two distinct metacommunities	0:27	Two distinct metacommunities characterize the gut microbiota in Crohn's disease patients.
28655159	3	73	theme	patients	342:349	arg1	number					329:334	a small number	321:334	a small number of CD patients	321:349	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	3	74	theme	microbiota	372:381	arg1	analyses					352:359	analyses	352:359	analyses of the gut microbiota in CD	352:387	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	1	75	theme	inflammatory	94:105	arg1	disorder					118:125	The inflammatory intestinal disorder Crohn's disease (CD)	90:146	The inflammatory intestinal disorder Crohn's disease (CD)	90:146	The inflammatory intestinal disorder Crohn's disease (CD) has become a health challenge worldwide.
28655159	7	76	from	reduction	1111:1119	arg1	rates					1131:1135	growth rates	1124:1135	growth rates of many bacterial species	1124:1161	Disruption of ecological networks was evident in CD, coupled with reduction in growth rates of many bacterial species.
28655159	3	77	theme	rDNA	403:406	arg1	sequencing					417:426	16S rDNA amplicon sequencing	399:426	16S rDNA amplicon sequencing	399:426	Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing.
28655159	0	78	theme	gut	46:48	arg1	microbiota					50:59	the gut microbiota	42:59	the gut microbiota in Crohn's disease patients	42:87	Two distinct metacommunities characterize the gut microbiota in Crohn's disease patients.
28655159	8	79	theme	overall	1234:1240	arg1	composition					1242:1252	the overall composition	1230:1252	the overall composition of the CD microbiota	1230:1273	Short-term exclusive enteral nutrition elicited limited impact on the overall composition of the CD microbiota, although functional changes occurred following treatment.
28655159	7	80	theme	bacterial	1145:1153	arg1	species					1155:1161	many bacterial species	1140:1161	many bacterial species	1140:1161	Disruption of ecological networks was evident in CD, coupled with reduction in growth rates of many bacterial species.
28655159	6	81	theme	Metacommunity-specific	830:851	arg1	shifts					864:869	Metacommunity-specific functional shifts	830:869	Metacommunity-specific functional shifts in CD	830:875	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	2	82	theme	gut	193:195	arg1	microbiota					197:206	The gut microbiota	189:206	The gut microbiota	189:206	The gut microbiota closely interacts with the host immune system, but its functional impact in CD is unclear.
28655159	6	83	from	variant	965:971	arg1	potential					996:1004	the potential to synthesize short-chain fatty acids	992:1042	the potential to synthesize short-chain fatty acids	992:1042	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	4	84	theme	microbiota	571:580	arg1	features					552:559	the compositional and functional features	519:559	the compositional and functional features	519:559	Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition.
28655159	6	85	from	producers	898:906	arg1	potential					996:1004	the potential to synthesize short-chain fatty acids	992:1042	the potential to synthesize short-chain fatty acids	992:1042	Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids.
28655159	9	86	theme	perturbed	1439:1447	arg1	metacommunity					1449:1461	the most severely perturbed metacommunity	1421:1461	the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis	1421:1611	The microbiotas in CD patients can be stratified into 2 distinct metacommunities, with the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis.
28946905	9	0	from	changes	1296:1302	arg1	flux					1318:1321	glycolytic flux	1307:1321	glycolytic flux	1307:1321	This response indicates that the storage metabolism is very sensitive to changes in glycolytic flux and counterbalances these rapid changes by diverting flux into large pools to prevent substrate accelerated death and potentially refill the central metabolism when substrates become scarce.
28946905	12	1	theme	inflow	1829:1834	arg1	inflow					1829:1834	the carbon inflow	1818:1834	the carbon inflow	1818:1834	Based on the 13C labeling in average 15% of the carbon inflow is recycled via trehalose and glycogen.
28946905	12	1	theme	inflow	1829:1834	arg1	%					1813:1813	average 15%	1803:1813	average 15% of the carbon inflow	1803:1834	Based on the 13C labeling in average 15% of the carbon inflow is recycled via trehalose and glycogen.
28946905	10	2	theme	G6P	1593:1595	arg1	labeling					1558:1565	the labeling	1554:1565	the labeling of extracellular glucose, G6P, T6P and other metabolites	1554:1622	Using 13C-tracer we found a dilution in the labeling of extracellular glucose, G6P, T6P and other metabolites, indicating an influx of unlabeled carbon.
28946905	18	3	theme	slight	2579:2584	arg1	changes					2586:2592	slight changes	2579:2592	slight changes in the biomass composition	2579:2619	Combined with slight changes in the biomass composition, the yield decrease of 5% can be explained.
28946905	3	4	from	perturbations	550:562	arg1	availability					577:588	substrate availability	567:588	substrate availability	567:588	RESULTS The metabolic flux reacts fast and sensitive to cyclic perturbations in substrate availability.
28946905	2	5	theme	carbon	366:371	arg1	metabolism					373:382	central carbon metabolism	358:382	central carbon metabolism	358:382	Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae.
28946905	12	6	theme	average	1803:1809	arg1	inflow					1829:1834	the carbon inflow	1818:1834	the carbon inflow	1818:1834	Based on the 13C labeling in average 15% of the carbon inflow is recycled via trehalose and glycogen.
28946905	12	6	theme	average	1803:1809	arg1	%					1813:1813	average 15%	1803:1813	average 15% of the carbon inflow	1803:1834	Based on the 13C labeling in average 15% of the carbon inflow is recycled via trehalose and glycogen.
28946905	4	7	theme	metabolic	683:691	arg1	responses					693:701	different metabolic responses	673:701	different metabolic responses	673:701	Compared to well-documented stimulus-response experiments using substrate pulses, different metabolic responses are observed.
28946905	6	8	theme	high	880:883	arg1	flux					896:899	a high glycolytic flux	878:899	a high glycolytic flux of up to 5.4 mmol g DW-1 h-1	878:928	Although a high glycolytic flux of up to 5.4 mmol g DW-1 h-1 is observed, no overflow metabolites are detected.
28946905	14	9	dep	peaks	2154:2158	arg1	to					2144:2145	to	2144:2145	to	2144:2145	CONCLUSIONS Comparable to electric energy grids, metabolism seems to use storage units to buffer peaks and keep reserves to maintain a robust function.
28946905	17	10	theme	reversed	2487:2494	arg1	flux					2496:2499	a reversed flux	2485:2499	a reversed flux of transaldolase	2485:2516	Most remarkably is a fivefold increased flux towards PPP that generated a reversed flux of transaldolase and the F6P-producing transketolase reactions.
28946905	10	11	theme	other	1606:1610	arg1	metabolites					1612:1622	other metabolites	1606:1622	other metabolites	1606:1622	Using 13C-tracer we found a dilution in the labeling of extracellular glucose, G6P, T6P and other metabolites, indicating an influx of unlabeled carbon.
28946905	1	12	theme	other	199:203	arg1	conditions					205:214	other conditions	199:214	other conditions	199:214	BACKGROUND Natural and industrial environments are dynamic with respect to substrate availability and other conditions like temperature and pH. Especially, metabolism is strongly affected by changes in the extracellular space.
28946905	9	13	theme	storage	1256:1262	arg1	sensitive					1283:1291	sensitive	1283:1291	sensitive	1283:1291	This response indicates that the storage metabolism is very sensitive to changes in glycolytic flux and counterbalances these rapid changes by diverting flux into large pools to prevent substrate accelerated death and potentially refill the central metabolism when substrates become scarce.
28946905	9	13	theme	storage	1256:1262	arg1	metabolism					1264:1273	the storage metabolism	1252:1273	the storage metabolism	1252:1273	This response indicates that the storage metabolism is very sensitive to changes in glycolytic flux and counterbalances these rapid changes by diverting flux into large pools to prevent substrate accelerated death and potentially refill the central metabolism when substrates become scarce.
28946905	14	14	theme	robust	2192:2197	arg1	function					2199:2206	a robust function	2190:2206	a robust function	2190:2206	CONCLUSIONS Comparable to electric energy grids, metabolism seems to use storage units to buffer peaks and keep reserves to maintain a robust function.
28946905	14	15	dep	CONCLUSIONS	2057:2067	arg1	seems					2117:2121	seems	2117:2121	seems to use storage units to buffer peaks and keep reserves to maintain a robust function	2117:2206	CONCLUSIONS Comparable to electric energy grids, metabolism seems to use storage units to buffer peaks and keep reserves to maintain a robust function.
28946905	17	16	theme	F6P-producing	2526:2538	arg1	reactions					2554:2562	the F6P-producing transketolase reactions	2522:2562	the F6P-producing transketolase reactions	2522:2562	Most remarkably is a fivefold increased flux towards PPP that generated a reversed flux of transaldolase and the F6P-producing transketolase reactions.
28946905	7	17	theme	more	1100:1103	arg1	changes					1113:1119	even more drastic changes	1095:1119	even more drastic changes	1095:1119	From famine to feast the glucose uptake rate increased from 170 to 4788 μmol g DW-1 h-1 in 24 s. Intracellularly, even more drastic changes were observed.
28946905	7	18	theme	DW-1	1060:1063	arg1	h-1					1065:1067	170 to 4788 μmol g DW-1 h-1	1041:1067	170 to 4788 μmol g DW-1 h-1	1041:1067	From famine to feast the glucose uptake rate increased from 170 to 4788 μmol g DW-1 h-1 in 24 s. Intracellularly, even more drastic changes were observed.
28946905	9	19	theme	large	1386:1390	arg1	pools					1392:1396	large pools	1386:1396	large pools to prevent substrate accelerated death and potentially refill the central metabolism when substrates become scarce	1386:1511	This response indicates that the storage metabolism is very sensitive to changes in glycolytic flux and counterbalances these rapid changes by diverting flux into large pools to prevent substrate accelerated death and potentially refill the central metabolism when substrates become scarce.
28946905	10	20	theme	carbon	1659:1664	arg1	influx					1639:1644	an influx	1636:1644	an influx of unlabeled carbon	1636:1664	Using 13C-tracer we found a dilution in the labeling of extracellular glucose, G6P, T6P and other metabolites, indicating an influx of unlabeled carbon.
28946905	5	21	from	increase	842:849	arg1	charge					861:866	energy charge	854:866	energy charge	854:866	Especially, cells experiencing cyclic perturbations do not show a drop in ATP with the addition of glucose, but an immediate increase in energy charge.
28946905	9	22	theme	glycolytic	1307:1316	arg1	flux					1318:1321	glycolytic flux	1307:1321	glycolytic flux	1307:1321	This response indicates that the storage metabolism is very sensitive to changes in glycolytic flux and counterbalances these rapid changes by diverting flux into large pools to prevent substrate accelerated death and potentially refill the central metabolism when substrates become scarce.
28946905	5	23	theme	immediate	832:840	arg1	increase					842:849	an immediate increase	829:849	an immediate increase in energy charge	829:866	Especially, cells experiencing cyclic perturbations do not show a drop in ATP with the addition of glucose, but an immediate increase in energy charge.
28946905	15	24	theme	feast/famine	2233:2244	arg1	conditions					2246:2255	the applied fast feast/famine conditions	2216:2255	the applied fast feast/famine conditions	2216:2255	During the applied fast feast/famine conditions about 15% of the metabolized carbon were recycled in storage metabolism.
28946905	4	25	theme	substrate	655:663	arg1	pulses					665:670	substrate pulses	655:670	substrate pulses	655:670	Compared to well-documented stimulus-response experiments using substrate pulses, different metabolic responses are observed.
28946905	13	26	theme	steady-state	1919:1930	arg1	turnover					1932:1939	the steady-state turnover	1915:1939	the steady-state turnover	1915:1939	This average fraction is comparable to the steady-state turnover, but changes significantly during the cycle, indicating the relevance for dynamic regulation of the metabolic flux.
28946905	7	27	theme	uptake	1014:1019	arg1	rate					1021:1024	the glucose uptake rate	1002:1024	the glucose uptake rate	1002:1024	From famine to feast the glucose uptake rate increased from 170 to 4788 μmol g DW-1 h-1 in 24 s. Intracellularly, even more drastic changes were observed.
28946905	15	28	theme	applied	2220:2226	arg1	conditions					2246:2255	the applied fast feast/famine conditions	2216:2255	the applied fast feast/famine conditions	2216:2255	During the applied fast feast/famine conditions about 15% of the metabolized carbon were recycled in storage metabolism.
28946905	7	29	dep	feast	996:1000	arg1	famine					986:991	famine	986:991	famine	986:991	From famine to feast the glucose uptake rate increased from 170 to 4788 μmol g DW-1 h-1 in 24 s. Intracellularly, even more drastic changes were observed.
28946905	5	30	theme	glucose	816:822	arg1	addition					804:811	the addition	800:811	the addition of glucose	800:822	Especially, cells experiencing cyclic perturbations do not show a drop in ATP with the addition of glucose, but an immediate increase in energy charge.
28946905	2	31	theme	dynamic	426:432	arg1	conditions					447:456	dynamic feast/famine conditions	426:456	dynamic feast/famine conditions in Saccharomyces cerevisiae	426:484	Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae.
28946905	15	32	theme	storage	2310:2316	arg1	metabolism					2318:2327	storage metabolism	2310:2327	storage metabolism	2310:2327	During the applied fast feast/famine conditions about 15% of the metabolized carbon were recycled in storage metabolism.
28946905	14	33	theme	storage	2130:2136	arg1	peaks					2154:2158	storage units to buffer peaks	2130:2158	storage units to buffer peaks	2130:2158	CONCLUSIONS Comparable to electric energy grids, metabolism seems to use storage units to buffer peaks and keep reserves to maintain a robust function.
28946905	2	34	theme	metabolism	409:418	arg1	flux					350:353	the dynamic flux	338:353	the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae	338:484	Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae.
28946905	0	35	theme	Metabolic	0:8	arg1	adjustment					10:19	Metabolic adjustment	0:19	Metabolic adjustment upon repetitive substrate perturbations	0:59	Metabolic adjustment upon repetitive substrate perturbations using dynamic 13C-tracing in yeast.
28946905	17	36	theme	increased	2443:2451	arg1	flux					2453:2456	a fivefold increased flux	2432:2456	a fivefold increased flux towards PPP that generated a reversed flux of transaldolase and the F6P-producing transketolase reactions	2432:2562	Most remarkably is a fivefold increased flux towards PPP that generated a reversed flux of transaldolase and the F6P-producing transketolase reactions.
28946905	1	37	theme	Natural	108:114	arg1	environments					131:142	BACKGROUND Natural and industrial environments	97:142	BACKGROUND Natural and industrial environments	97:142	BACKGROUND Natural and industrial environments are dynamic with respect to substrate availability and other conditions like temperature and pH. Especially, metabolism is strongly affected by changes in the extracellular space.
28946905	4	38	theme	stimulus-response	619:635	arg1	experiments					637:647	well-documented stimulus-response experiments	603:647	well-documented stimulus-response experiments using substrate pulses	603:670	Compared to well-documented stimulus-response experiments using substrate pulses, different metabolic responses are observed.
28946905	16	39	theme	steady-state	2388:2399	arg1	conditions					2401:2410	steady-state conditions	2388:2410	steady-state conditions	2388:2410	Additionally, the resources were distributed different to steady-state conditions.
28946905	2	40	theme	Saccharomyces	461:473	arg1	cerevisiae					475:484	Saccharomyces cerevisiae	461:484	Saccharomyces cerevisiae	461:484	Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae.
28946905	10	41	dep	found	1534:1538	arg1	Using					1514:1518	Using	1514:1518	Using 13C-tracer	1514:1529	Using 13C-tracer we found a dilution in the labeling of extracellular glucose, G6P, T6P and other metabolites, indicating an influx of unlabeled carbon.
28946905	11	42	dep	glycogen	1684:1691	arg1	degradation					1707:1717	degradation	1707:1717	degradation	1707:1717	It is shown that glycogen and trehalose degradation via different routes could explain these observations.
28946905	1	43	theme	industrial	120:129	arg1	environments					131:142	BACKGROUND Natural and industrial environments	97:142	BACKGROUND Natural and industrial environments	97:142	BACKGROUND Natural and industrial environments are dynamic with respect to substrate availability and other conditions like temperature and pH. Especially, metabolism is strongly affected by changes in the extracellular space.
28946905	12	44	from	labeling	1791:1798	arg1	inflow					1829:1834	the carbon inflow	1818:1834	the carbon inflow	1818:1834	Based on the 13C labeling in average 15% of the carbon inflow is recycled via trehalose and glycogen.
28946905	12	44	from	labeling	1791:1798	arg1	%					1813:1813	average 15%	1803:1813	average 15% of the carbon inflow	1803:1834	Based on the 13C labeling in average 15% of the carbon inflow is recycled via trehalose and glycogen.
28946905	13	45	theme	average	1881:1887	arg1	fraction					1889:1896	This average fraction	1876:1896	This average fraction	1876:1896	This average fraction is comparable to the steady-state turnover, but changes significantly during the cycle, indicating the relevance for dynamic regulation of the metabolic flux.
28946905	3	46	theme	metabolic	499:507	arg1	flux					509:512	The metabolic flux	495:512	The metabolic flux	495:512	RESULTS The metabolic flux reacts fast and sensitive to cyclic perturbations in substrate availability.
28946905	0	47	theme	substrate	37:45	arg1	perturbations					47:59	repetitive substrate perturbations	26:59	repetitive substrate perturbations	26:59	Metabolic adjustment upon repetitive substrate perturbations using dynamic 13C-tracing in yeast.
28946905	15	48	theme	metabolized	2274:2284	arg1	carbon					2286:2291	the metabolized carbon	2270:2291	the metabolized carbon	2270:2291	During the applied fast feast/famine conditions about 15% of the metabolized carbon were recycled in storage metabolism.
28946905	6	49	theme	DW-1	921:924	arg1	h-1					926:928	up to 5.4 mmol g DW-1 h-1	904:928	up to 5.4 mmol g DW-1 h-1	904:928	Although a high glycolytic flux of up to 5.4 mmol g DW-1 h-1 is observed, no overflow metabolites are detected.
28946905	2	50	theme	central	358:364	arg1	metabolism					373:382	central carbon metabolism	358:382	central carbon metabolism	358:382	Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae.
28946905	9	51	theme	accelerated	1419:1429	arg1	death					1431:1435	substrate accelerated death	1409:1435	substrate accelerated death	1409:1435	This response indicates that the storage metabolism is very sensitive to changes in glycolytic flux and counterbalances these rapid changes by diverting flux into large pools to prevent substrate accelerated death and potentially refill the central metabolism when substrates become scarce.
28946905	17	52	theme	transketolase	2540:2552	arg1	reactions					2554:2562	the F6P-producing transketolase reactions	2522:2562	the F6P-producing transketolase reactions	2522:2562	Most remarkably is a fivefold increased flux towards PPP that generated a reversed flux of transaldolase and the F6P-producing transketolase reactions.
28946905	10	53	theme	extracellular	1570:1582	arg1	glucose					1584:1590	extracellular glucose	1570:1590	extracellular glucose	1570:1590	Using 13C-tracer we found a dilution in the labeling of extracellular glucose, G6P, T6P and other metabolites, indicating an influx of unlabeled carbon.
28946905	5	54	from	drop	783:786	arg1	ATP					791:793	ATP	791:793	ATP with the addition of glucose	791:822	Especially, cells experiencing cyclic perturbations do not show a drop in ATP with the addition of glucose, but an immediate increase in energy charge.
28946905	8	55	theme	T6P	1152:1154	arg1	rate					1166:1169	the T6P synthesis rate	1148:1169	the T6P synthesis rate	1148:1169	Especially, the T6P synthesis rate increased more than 100-fold upon glucose addition.
28946905	2	56	from	conditions	447:456	arg1	cerevisiae					475:484	Saccharomyces cerevisiae	461:484	Saccharomyces cerevisiae	461:484	Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae.
28946905	3	57	theme	substrate	567:575	arg1	availability					577:588	substrate availability	567:588	substrate availability	567:588	RESULTS The metabolic flux reacts fast and sensitive to cyclic perturbations in substrate availability.
28946905	15	58	theme	carbon	2286:2291	arg1	carbon					2286:2291	the metabolized carbon	2270:2291	the metabolized carbon	2270:2291	During the applied fast feast/famine conditions about 15% of the metabolized carbon were recycled in storage metabolism.
28946905	15	58	theme	carbon	2286:2291	arg1	%					2265:2265	about 15%	2257:2265	about 15% of the metabolized carbon	2257:2291	During the applied fast feast/famine conditions about 15% of the metabolized carbon were recycled in storage metabolism.
28946905	18	59	theme	%	2645:2645	arg1	decrease					2632:2639	the yield decrease	2622:2639	the yield decrease of 5%	2622:2645	Combined with slight changes in the biomass composition, the yield decrease of 5% can be explained.
28946905	5	60	theme	cyclic	748:753	arg1	perturbations					755:767	cyclic perturbations	748:767	cyclic perturbations	748:767	Especially, cells experiencing cyclic perturbations do not show a drop in ATP with the addition of glucose, but an immediate increase in energy charge.
28946905	13	61	theme	metabolic	2041:2049	arg1	flux					2051:2054	the metabolic flux	2037:2054	the metabolic flux	2037:2054	This average fraction is comparable to the steady-state turnover, but changes significantly during the cycle, indicating the relevance for dynamic regulation of the metabolic flux.
28946905	2	62	theme	metabolism	373:382	arg1	flux					350:353	the dynamic flux	338:353	the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae	338:484	Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae.
28946905	9	63	theme	central	1464:1470	arg1	metabolism					1472:1481	the central metabolism	1460:1481	the central metabolism	1460:1481	This response indicates that the storage metabolism is very sensitive to changes in glycolytic flux and counterbalances these rapid changes by diverting flux into large pools to prevent substrate accelerated death and potentially refill the central metabolism when substrates become scarce.
28946905	6	64	theme	glycolytic	885:894	arg1	flux					896:899	a high glycolytic flux	878:899	a high glycolytic flux of up to 5.4 mmol g DW-1 h-1	878:928	Although a high glycolytic flux of up to 5.4 mmol g DW-1 h-1 is observed, no overflow metabolites are detected.
28946905	6	65	theme	overflow	946:953	arg1	metabolites					955:965	no overflow metabolites	943:965	no overflow metabolites	943:965	Although a high glycolytic flux of up to 5.4 mmol g DW-1 h-1 is observed, no overflow metabolites are detected.
28946905	4	66	theme	different	673:681	arg1	responses					693:701	different metabolic responses	673:701	different metabolic responses	673:701	Compared to well-documented stimulus-response experiments using substrate pulses, different metabolic responses are observed.
28946905	10	67	theme	metabolites	1612:1622	arg1	labeling					1558:1565	the labeling	1554:1565	the labeling of extracellular glucose, G6P, T6P and other metabolites	1554:1622	Using 13C-tracer we found a dilution in the labeling of extracellular glucose, G6P, T6P and other metabolites, indicating an influx of unlabeled carbon.
28946905	11	68	theme	different	1723:1731	arg1	routes					1733:1738	different routes	1723:1738	different routes	1723:1738	It is shown that glycogen and trehalose degradation via different routes could explain these observations.
28946905	13	69	theme	dynamic	2015:2021	arg1	regulation					2023:2032	dynamic regulation	2015:2032	dynamic regulation of the metabolic flux	2015:2054	This average fraction is comparable to the steady-state turnover, but changes significantly during the cycle, indicating the relevance for dynamic regulation of the metabolic flux.
28946905	8	70	theme	glucose	1205:1211	arg1	addition					1213:1220	glucose addition	1205:1220	glucose addition	1205:1220	Especially, the T6P synthesis rate increased more than 100-fold upon glucose addition.
28946905	12	71	theme	13C	1787:1789	arg1	labeling					1791:1798	the 13C labeling	1783:1798	the 13C labeling in average 15% of the carbon inflow	1783:1834	Based on the 13C labeling in average 15% of the carbon inflow is recycled via trehalose and glycogen.
28946905	10	72	theme	unlabeled	1649:1657	arg1	carbon					1659:1664	unlabeled carbon	1649:1664	unlabeled carbon	1649:1664	Using 13C-tracer we found a dilution in the labeling of extracellular glucose, G6P, T6P and other metabolites, indicating an influx of unlabeled carbon.
28946905	14	73	theme	energy	2092:2097	arg1	grids					2099:2103	electric energy grids	2083:2103	electric energy grids	2083:2103	CONCLUSIONS Comparable to electric energy grids, metabolism seems to use storage units to buffer peaks and keep reserves to maintain a robust function.
28946905	17	74	theme	transaldolase	2504:2516	arg1	flux					2496:2499	a reversed flux	2485:2499	a reversed flux of transaldolase	2485:2516	Most remarkably is a fivefold increased flux towards PPP that generated a reversed flux of transaldolase and the F6P-producing transketolase reactions.
28946905	17	74	theme	transaldolase	2504:2516	arg1	reactions					2554:2562	the F6P-producing transketolase reactions	2522:2562	the F6P-producing transketolase reactions	2522:2562	Most remarkably is a fivefold increased flux towards PPP that generated a reversed flux of transaldolase and the F6P-producing transketolase reactions.
28946905	18	75	theme	biomass	2601:2607	arg1	composition					2609:2619	the biomass composition	2597:2619	the biomass composition	2597:2619	Combined with slight changes in the biomass composition, the yield decrease of 5% can be explained.
28946905	1	76	from	changes	288:294	arg1	space					317:321	the extracellular space	299:321	the extracellular space	299:321	BACKGROUND Natural and industrial environments are dynamic with respect to substrate availability and other conditions like temperature and pH. Especially, metabolism is strongly affected by changes in the extracellular space.
28946905	18	77	theme	yield	2626:2630	arg1	decrease					2632:2639	the yield decrease	2622:2639	the yield decrease of 5%	2622:2645	Combined with slight changes in the biomass composition, the yield decrease of 5% can be explained.
28946905	14	78	theme	electric	2083:2090	arg1	grids					2099:2103	electric energy grids	2083:2103	electric energy grids	2083:2103	CONCLUSIONS Comparable to electric energy grids, metabolism seems to use storage units to buffer peaks and keep reserves to maintain a robust function.
28946905	7	79	theme	drastic	1105:1111	arg1	changes					1113:1119	even more drastic changes	1095:1119	even more drastic changes	1095:1119	From famine to feast the glucose uptake rate increased from 170 to 4788 μmol g DW-1 h-1 in 24 s. Intracellularly, even more drastic changes were observed.
28946905	7	80	theme	g	1058:1058	arg1	h-1					1065:1067	170 to 4788 μmol g DW-1 h-1	1041:1067	170 to 4788 μmol g DW-1 h-1	1041:1067	From famine to feast the glucose uptake rate increased from 170 to 4788 μmol g DW-1 h-1 in 24 s. Intracellularly, even more drastic changes were observed.
28946905	15	81	theme	fast	2228:2231	arg1	conditions					2246:2255	the applied fast feast/famine conditions	2216:2255	the applied fast feast/famine conditions	2216:2255	During the applied fast feast/famine conditions about 15% of the metabolized carbon were recycled in storage metabolism.
28946905	1	82	theme	substrate	172:180	arg1	availability					182:193	substrate availability	172:193	substrate availability	172:193	BACKGROUND Natural and industrial environments are dynamic with respect to substrate availability and other conditions like temperature and pH. Especially, metabolism is strongly affected by changes in the extracellular space.
28946905	9	83	theme	rapid	1349:1353	arg1	changes					1355:1361	these rapid changes	1343:1361	these rapid changes	1343:1361	This response indicates that the storage metabolism is very sensitive to changes in glycolytic flux and counterbalances these rapid changes by diverting flux into large pools to prevent substrate accelerated death and potentially refill the central metabolism when substrates become scarce.
28946905	12	84	theme	carbon	1822:1827	arg1	inflow					1829:1834	the carbon inflow	1818:1834	the carbon inflow	1818:1834	Based on the 13C labeling in average 15% of the carbon inflow is recycled via trehalose and glycogen.
28946905	14	85	theme	buffer	2147:2152	arg1	peaks					2154:2158	storage units to buffer peaks	2130:2158	storage units to buffer peaks	2130:2158	CONCLUSIONS Comparable to electric energy grids, metabolism seems to use storage units to buffer peaks and keep reserves to maintain a robust function.
28946905	7	86	dep	4788 μmol	1048:1056	arg1	to					1045:1046	to	1045:1046	to	1045:1046	From famine to feast the glucose uptake rate increased from 170 to 4788 μmol g DW-1 h-1 in 24 s. Intracellularly, even more drastic changes were observed.
28946905	7	87	theme	glucose	1006:1012	arg1	rate					1021:1024	the glucose uptake rate	1002:1024	the glucose uptake rate	1002:1024	From famine to feast the glucose uptake rate increased from 170 to 4788 μmol g DW-1 h-1 in 24 s. Intracellularly, even more drastic changes were observed.
28946905	14	88	theme	units	2138:2142	arg1	peaks					2154:2158	storage units to buffer peaks	2130:2158	storage units to buffer peaks	2130:2158	CONCLUSIONS Comparable to electric energy grids, metabolism seems to use storage units to buffer peaks and keep reserves to maintain a robust function.
28946905	2	89	theme	carbohydrate	396:407	arg1	metabolism					409:418	storage carbohydrate metabolism	388:418	storage carbohydrate metabolism	388:418	Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae.
28946905	0	90	theme	repetitive	26:35	arg1	perturbations					47:59	repetitive substrate perturbations	26:59	repetitive substrate perturbations	26:59	Metabolic adjustment upon repetitive substrate perturbations using dynamic 13C-tracing in yeast.
28946905	1	91	theme	extracellular	303:315	arg1	space					317:321	the extracellular space	299:321	the extracellular space	299:321	BACKGROUND Natural and industrial environments are dynamic with respect to substrate availability and other conditions like temperature and pH. Especially, metabolism is strongly affected by changes in the extracellular space.
28946905	6	92	dep	5.4 mmol	910:917	arg1	to					907:908	to	907:908	to	907:908	Although a high glycolytic flux of up to 5.4 mmol g DW-1 h-1 is observed, no overflow metabolites are detected.
28946905	2	93	theme	storage	388:394	arg1	metabolism					409:418	storage carbohydrate metabolism	388:418	storage carbohydrate metabolism	388:418	Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae.
28946905	5	94	theme	energy	854:859	arg1	charge					861:866	energy charge	854:866	energy charge	854:866	Especially, cells experiencing cyclic perturbations do not show a drop in ATP with the addition of glucose, but an immediate increase in energy charge.
28946905	6	95	theme	h-1	926:928	arg1	flux					896:899	a high glycolytic flux	878:899	a high glycolytic flux of up to 5.4 mmol g DW-1 h-1	878:928	Although a high glycolytic flux of up to 5.4 mmol g DW-1 h-1 is observed, no overflow metabolites are detected.
28946905	0	96	from	13C-tracing	75:85	arg1	yeast					90:94	yeast	90:94	yeast	90:94	Metabolic adjustment upon repetitive substrate perturbations using dynamic 13C-tracing in yeast.
28946905	5	97	with	ATP	791:793	arg1	addition					804:811	the addition	800:811	the addition of glucose	800:822	Especially, cells experiencing cyclic perturbations do not show a drop in ATP with the addition of glucose, but an immediate increase in energy charge.
28946905	13	98	theme	flux	2051:2054	arg1	regulation					2023:2032	dynamic regulation	2015:2032	dynamic regulation of the metabolic flux	2015:2054	This average fraction is comparable to the steady-state turnover, but changes significantly during the cycle, indicating the relevance for dynamic regulation of the metabolic flux.
28946905	2	99	theme	feast/famine	434:445	arg1	conditions					447:456	dynamic feast/famine conditions	426:456	dynamic feast/famine conditions in Saccharomyces cerevisiae	426:484	Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae.
28946905	0	100	theme	dynamic	67:73	arg1	13C-tracing					75:85	dynamic 13C-tracing	67:85	dynamic 13C-tracing in yeast	67:94	Metabolic adjustment upon repetitive substrate perturbations using dynamic 13C-tracing in yeast.
28946905	9	101	theme	substrate	1409:1417	arg1	death					1431:1435	substrate accelerated death	1409:1435	substrate accelerated death	1409:1435	This response indicates that the storage metabolism is very sensitive to changes in glycolytic flux and counterbalances these rapid changes by diverting flux into large pools to prevent substrate accelerated death and potentially refill the central metabolism when substrates become scarce.
28946905	6	102	theme	g	919:919	arg1	h-1					926:928	up to 5.4 mmol g DW-1 h-1	904:928	up to 5.4 mmol g DW-1 h-1	904:928	Although a high glycolytic flux of up to 5.4 mmol g DW-1 h-1 is observed, no overflow metabolites are detected.
28946905	3	103	dep	RESULTS	487:493	arg1	reacts					514:519	reacts	514:519	reacts fast and sensitive to cyclic perturbations in substrate availability	514:588	RESULTS The metabolic flux reacts fast and sensitive to cyclic perturbations in substrate availability.
28946905	4	104	theme	well-documented	603:617	arg1	experiments					637:647	well-documented stimulus-response experiments	603:647	well-documented stimulus-response experiments using substrate pulses	603:670	Compared to well-documented stimulus-response experiments using substrate pulses, different metabolic responses are observed.
28946905	10	105	theme	glucose	1584:1590	arg1	labeling					1558:1565	the labeling	1554:1565	the labeling of extracellular glucose, G6P, T6P and other metabolites	1554:1622	Using 13C-tracer we found a dilution in the labeling of extracellular glucose, G6P, T6P and other metabolites, indicating an influx of unlabeled carbon.
28946905	3	106	theme	cyclic	543:548	arg1	perturbations					550:562	cyclic perturbations	543:562	cyclic perturbations in substrate availability	543:588	RESULTS The metabolic flux reacts fast and sensitive to cyclic perturbations in substrate availability.
28946905	18	107	from	changes	2586:2592	arg1	composition					2609:2619	the biomass composition	2597:2619	the biomass composition	2597:2619	Combined with slight changes in the biomass composition, the yield decrease of 5% can be explained.
28946905	8	108	theme	synthesis	1156:1164	arg1	rate					1166:1169	the T6P synthesis rate	1148:1169	the T6P synthesis rate	1148:1169	Especially, the T6P synthesis rate increased more than 100-fold upon glucose addition.
28946905	2	109	theme	dynamic	342:348	arg1	flux					350:353	the dynamic flux	338:353	the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae	338:484	Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae.
28946905	10	110	theme	T6P	1598:1600	arg1	labeling					1558:1565	the labeling	1554:1565	the labeling of extracellular glucose, G6P, T6P and other metabolites	1554:1622	Using 13C-tracer we found a dilution in the labeling of extracellular glucose, G6P, T6P and other metabolites, indicating an influx of unlabeled carbon.
27833472	10	0	theme	SP	1393:1394	arg1	cells					1396:1400	SP cells	1393:1400	SP cells	1393:1400	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	5	1	theme	SP	683:684	arg1	cells					686:690	SP cells	683:690	SP cells	683:690	Here, we isolated SP cells from ovarian cancer cell lines and analyzed their glycosylation patterns using multiple glycomic strategies.
27833472	6	2	theme	cancer	839:844	arg1	lines					851:855	Six high-grade serous ovarian cancer cell lines	809:855	METHODS Six high-grade serous ovarian cancer cell lines	801:855	METHODS Six high-grade serous ovarian cancer cell lines were used for SP cell isolation.
27833472	12	3	theme	stem	1640:1643	arg1	cells					1645:1649	ovarian cancer stem cells	1625:1649	ovarian cancer stem cells	1625:1649	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	6	4	dep	METHODS	801:807	arg1	lines					851:855	Six high-grade serous ovarian cancer cell lines	809:855	METHODS Six high-grade serous ovarian cancer cell lines	801:855	METHODS Six high-grade serous ovarian cancer cell lines were used for SP cell isolation.
27833472	6	5	theme	serous	824:829	arg1	lines					851:855	Six high-grade serous ovarian cancer cell lines	809:855	METHODS Six high-grade serous ovarian cancer cell lines	801:855	METHODS Six high-grade serous ovarian cancer cell lines were used for SP cell isolation.
27833472	2	6	theme	cancer	298:303	arg1	cells					305:309	cancer cells	298:309	cancer cells	298:309	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	1	7	theme	drug	175:178	arg1	resistance					180:189	drug resistance	175:189	drug resistance	175:189	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	9	8	theme	flow	1214:1217	arg1	cytometry					1219:1227	flow cytometry	1214:1227	flow cytometry	1214:1227	Differently expressed glycan structures were further verified by lectin blot, flow cytometry, and real-time PCR analysis of their relevant enzymes.
27833472	10	9	gly	fucosylated	1311:1321	arg1	N-glycan					1323:1330	core fucosylated N-glycan	1306:1330	core fucosylated N-glycan	1306:1330	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	8	10	theme	Cell	1009:1012	arg1	lysate					1014:1019	Cell lysate	1009:1019	Cell lysate of SP cells and main population cells	1009:1057	Cell lysate of SP cells and main population cells was applied to lectin microarray and mass spectrometry for glycan profiling.
27833472	4	11	theme	cells	658:662	arg1	Changes					594:600	Changes	594:600	Changes in glycosylation patterns	594:626	Changes in glycosylation patterns are remarkable features of SP cells.
27833472	4	11	theme	cells	658:662	arg1	features					643:650	remarkable features	632:650	remarkable features of SP cells	632:662	Changes in glycosylation patterns are remarkable features of SP cells.
27833472	2	12	theme	cell-like	321:329	arg1	properties					331:340	stem cell-like properties	316:340	stem cell-like properties	316:340	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	1	13	theme	successful	202:211	arg1	treatment					221:229	successful initial treatment	202:229	successful initial treatment	202:229	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	9	14	theme	real-time	1234:1242	arg1	analysis					1248:1255	real-time PCR analysis	1234:1255	real-time PCR analysis	1234:1255	Differently expressed glycan structures were further verified by lectin blot, flow cytometry, and real-time PCR analysis of their relevant enzymes.
27833472	2	15	theme	scientific	245:254	arg1	evidence					256:263	Accumulating scientific evidence	232:263	Accumulating scientific evidence	232:263	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	11	16	link	α2,3-linked	1460:1470	arg1	glycan					1479:1484	α2,3-linked sialic glycan	1460:1484	α2,3-linked sialic glycan	1460:1484	By contrast, SP cells exhibited decreased hybrid glycan, α2,3-linked sialic glycan and multivalent sialyl-glycan.
27833472	7	17	theme	cells	1002:1006	arg1	analysis					987:994	glycomic analysis	978:994	glycomic analysis of SP cells	978:1006	Among them, HO8910 pm, which contained the highest proportion of SP cells, was used for glycomic analysis of SP cells.
27833472	1	18	theme	gynecological	115:127	arg1	malignancy					129:138	the most lethal gynecological malignancy	99:138	the most lethal gynecological malignancy due to its frequent recurrence and drug resistance	99:189	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	1	18	theme	gynecological	115:127	arg1	cancer					89:94	BACKGROUND Ovarian cancer	70:94	BACKGROUND Ovarian cancer	70:94	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	2	19	with	subpopulations	280:293	arg1	properties					331:340	stem cell-like properties	316:340	stem cell-like properties	316:340	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	5	20	theme	cancer	705:710	arg1	lines					717:721	ovarian cancer cell lines	697:721	ovarian cancer cell lines	697:721	Here, we isolated SP cells from ovarian cancer cell lines and analyzed their glycosylation patterns using multiple glycomic strategies.
27833472	1	21	theme	due	140:142	arg1	malignancy					129:138	the most lethal gynecological malignancy	99:138	the most lethal gynecological malignancy due to its frequent recurrence and drug resistance	99:189	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	1	21	theme	due	140:142	arg1	cancer					89:94	BACKGROUND Ovarian cancer	70:94	BACKGROUND Ovarian cancer	70:94	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	11	22	theme	sialic	1472:1477	arg1	glycan					1479:1484	α2,3-linked sialic glycan	1460:1484	α2,3-linked sialic glycan	1460:1484	By contrast, SP cells exhibited decreased hybrid glycan, α2,3-linked sialic glycan and multivalent sialyl-glycan.
27833472	5	23	theme	glycomic	780:787	arg1	strategies					789:798	multiple glycomic strategies	771:798	multiple glycomic strategies	771:798	Here, we isolated SP cells from ovarian cancer cell lines and analyzed their glycosylation patterns using multiple glycomic strategies.
27833472	9	24	theme	enzymes	1275:1281	arg1	analysis					1248:1255	real-time PCR analysis	1234:1255	real-time PCR analysis	1234:1255	Differently expressed glycan structures were further verified by lectin blot, flow cytometry, and real-time PCR analysis of their relevant enzymes.
27833472	9	24	theme	enzymes	1275:1281	arg1	blot					1208:1211	lectin blot	1201:1211	lectin blot	1201:1211	Differently expressed glycan structures were further verified by lectin blot, flow cytometry, and real-time PCR analysis of their relevant enzymes.
27833472	9	24	theme	enzymes	1275:1281	arg1	cytometry					1219:1227	flow cytometry	1214:1227	flow cytometry	1214:1227	Differently expressed glycan structures were further verified by lectin blot, flow cytometry, and real-time PCR analysis of their relevant enzymes.
27833472	7	25	theme	glycomic	978:985	arg1	analysis					987:994	glycomic analysis	978:994	glycomic analysis of SP cells	978:1006	Among them, HO8910 pm, which contained the highest proportion of SP cells, was used for glycomic analysis of SP cells.
27833472	12	26	theme	Glycan	1529:1534	arg1	antigens					1566:1573	sT antigens	1563:1573	sT antigens	1563:1573	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	26	theme	Glycan	1529:1534	arg1	biomarkers					1611:1620	biomarkers	1611:1620	biomarkers of ovarian cancer stem cells	1611:1649	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	26	theme	Glycan	1529:1534	arg1	Tn					1556:1557	Tn	1556:1557	Tn	1556:1557	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	26	theme	Glycan	1529:1534	arg1	T					1560:1560	T	1560:1560	T	1560:1560	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	26	theme	Glycan	1529:1534	arg1	fucosylation					1585:1596	core fucosylation	1580:1596	core fucosylation	1580:1596	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	26	theme	Glycan	1529:1534	arg1	structures					1536:1545	CONCLUSIONS Glycan structures	1517:1545	CONCLUSIONS Glycan structures	1517:1545	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	5	27	theme	glycosylation	742:754	arg1	patterns					756:763	their glycosylation patterns	736:763	their glycosylation patterns using multiple glycomic strategies	736:798	Here, we isolated SP cells from ovarian cancer cell lines and analyzed their glycosylation patterns using multiple glycomic strategies.
27833472	8	28	theme	cells	1027:1031	arg1	lysate					1014:1019	Cell lysate	1009:1019	Cell lysate of SP cells and main population cells	1009:1057	Cell lysate of SP cells and main population cells was applied to lectin microarray and mass spectrometry for glycan profiling.
27833472	11	29	theme	decreased	1435:1443	arg1	glycan					1452:1457	decreased hybrid glycan	1435:1457	decreased hybrid glycan	1435:1457	By contrast, SP cells exhibited decreased hybrid glycan, α2,3-linked sialic glycan and multivalent sialyl-glycan.
27833472	12	30	theme	cancer	1633:1638	arg1	cells					1645:1649	ovarian cancer stem cells	1625:1649	ovarian cancer stem cells	1625:1649	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	7	31	contain	contained	919:927	arg1	HO8910 pm					902:910	HO8910 pm	902:910	HO8910 pm	902:910	Among them, HO8910 pm, which contained the highest proportion of SP cells, was used for glycomic analysis of SP cells.
27833472	7	31	contain	contained	919:927	arg2	proportion					941:950	the highest proportion	929:950	the highest proportion of SP cells	929:962	Among them, HO8910 pm, which contained the highest proportion of SP cells, was used for glycomic analysis of SP cells.
27833472	4	32	from	Changes	594:600	arg1	patterns					619:626	glycosylation patterns	605:626	glycosylation patterns	605:626	Changes in glycosylation patterns are remarkable features of SP cells.
27833472	8	33	theme	main	1037:1040	arg1	cells					1053:1057	main population cells	1037:1057	main population cells	1037:1057	Cell lysate of SP cells and main population cells was applied to lectin microarray and mass spectrometry for glycan profiling.
27833472	7	34	theme	SP	955:956	arg1	cells					958:962	SP cells	955:962	SP cells	955:962	Among them, HO8910 pm, which contained the highest proportion of SP cells, was used for glycomic analysis of SP cells.
27833472	1	35	theme	Ovarian	81:87	arg1	malignancy					129:138	the most lethal gynecological malignancy	99:138	the most lethal gynecological malignancy due to its frequent recurrence and drug resistance	99:189	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	1	35	theme	Ovarian	81:87	arg1	cancer					89:94	BACKGROUND Ovarian cancer	70:94	BACKGROUND Ovarian cancer	70:94	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	12	36	theme	cells	1645:1649	arg1	antigens					1566:1573	sT antigens	1563:1573	sT antigens	1563:1573	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	36	theme	cells	1645:1649	arg1	biomarkers					1611:1620	biomarkers	1611:1620	biomarkers of ovarian cancer stem cells	1611:1649	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	36	theme	cells	1645:1649	arg1	Tn					1556:1557	Tn	1556:1557	Tn	1556:1557	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	36	theme	cells	1645:1649	arg1	T					1560:1560	T	1560:1560	T	1560:1560	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	36	theme	cells	1645:1649	arg1	fucosylation					1585:1596	core fucosylation	1580:1596	core fucosylation	1580:1596	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	36	theme	cells	1645:1649	arg1	structures					1536:1545	CONCLUSIONS Glycan structures	1517:1545	CONCLUSIONS Glycan structures	1517:1545	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	11	37	theme	SP	1416:1417	arg1	cells					1419:1423	SP cells	1416:1423	SP cells	1416:1423	By contrast, SP cells exhibited decreased hybrid glycan, α2,3-linked sialic glycan and multivalent sialyl-glycan.
27833472	6	38	used	used	862:865	arg2	METHODS					801:807	METHODS Six high-grade serous ovarian cancer cell lines	801:855	METHODS Six high-grade serous ovarian cancer cell lines	801:855	METHODS Six high-grade serous ovarian cancer cell lines were used for SP cell isolation.
27833472	8	39	theme	cells	1053:1057	arg1	lysate					1014:1019	Cell lysate	1009:1019	Cell lysate of SP cells and main population cells	1009:1057	Cell lysate of SP cells and main population cells was applied to lectin microarray and mass spectrometry for glycan profiling.
27833472	6	40	theme	ovarian	831:837	arg1	lines					851:855	Six high-grade serous ovarian cancer cell lines	809:855	METHODS Six high-grade serous ovarian cancer cell lines	801:855	METHODS Six high-grade serous ovarian cancer cell lines were used for SP cell isolation.
27833472	10	41	theme	fucosylated	1311:1321	arg1	N-glycan					1323:1330	core fucosylated N-glycan	1306:1330	core fucosylated N-glycan	1306:1330	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	11	42	theme	hybrid	1445:1450	arg1	glycan					1452:1457	decreased hybrid glycan	1435:1457	decreased hybrid glycan	1435:1457	By contrast, SP cells exhibited decreased hybrid glycan, α2,3-linked sialic glycan and multivalent sialyl-glycan.
27833472	3	43	theme	SP	464:465	arg1	cells					467:471	SP cells	464:471	SP cells	464:471	A better understanding of SP cells may provide new clues for detecting and targeting these cancer-initiating cells and ultimately help to eradicate cancer.
27833472	0	44	theme	ovarian	32:38	arg1	cells					63:67	ovarian cancer side population cells	32:67	ovarian cancer side population cells	32:67	Integrated glycomic analysis of ovarian cancer side population cells.
27833472	8	45	theme	lectin	1074:1079	arg1	microarray					1081:1090	lectin microarray	1074:1090	lectin microarray	1074:1090	Cell lysate of SP cells and main population cells was applied to lectin microarray and mass spectrometry for glycan profiling.
27833472	12	46	theme	CONCLUSIONS	1517:1527	arg1	antigens					1566:1573	sT antigens	1563:1573	sT antigens	1563:1573	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	46	theme	CONCLUSIONS	1517:1527	arg1	biomarkers					1611:1620	biomarkers	1611:1620	biomarkers of ovarian cancer stem cells	1611:1649	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	46	theme	CONCLUSIONS	1517:1527	arg1	Tn					1556:1557	Tn	1556:1557	Tn	1556:1557	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	46	theme	CONCLUSIONS	1517:1527	arg1	T					1560:1560	T	1560:1560	T	1560:1560	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	46	theme	CONCLUSIONS	1517:1527	arg1	fucosylation					1585:1596	core fucosylation	1580:1596	core fucosylation	1580:1596	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	12	46	theme	CONCLUSIONS	1517:1527	arg1	structures					1536:1545	CONCLUSIONS Glycan structures	1517:1545	CONCLUSIONS Glycan structures	1517:1545	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	0	47	theme	cells	63:67	arg1	analysis					20:27	glycomic analysis	11:27	glycomic analysis of ovarian cancer side population cells	11:67	Integrated glycomic analysis of ovarian cancer side population cells.
27833472	2	48	theme	population	366:375	arg1	cells					382:386	so-called side population (SP) cells	351:386	so-called side population (SP) cells	351:386	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	0	49	theme	side	47:50	arg1	cells					63:67	ovarian cancer side population cells	32:67	ovarian cancer side population cells	32:67	Integrated glycomic analysis of ovarian cancer side population cells.
27833472	6	50	theme	SP	871:872	arg1	isolation					879:887	SP cell isolation	871:887	SP cell isolation	871:887	METHODS Six high-grade serous ovarian cancer cell lines were used for SP cell isolation.
27833472	2	51	theme	so-called	351:359	arg1	cells					382:386	so-called side population (SP) cells	351:386	so-called side population (SP) cells	351:386	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	7	52	theme	highest	933:939	arg1	proportion					941:950	the highest proportion	929:950	the highest proportion of SP cells	929:962	Among them, HO8910 pm, which contained the highest proportion of SP cells, was used for glycomic analysis of SP cells.
27833472	9	53	theme	glycan	1158:1163	arg1	structures					1165:1174	Differently expressed glycan structures	1136:1174	Differently expressed glycan structures	1136:1174	Differently expressed glycan structures were further verified by lectin blot, flow cytometry, and real-time PCR analysis of their relevant enzymes.
27833472	8	54	theme	glycan	1118:1123	arg1	profiling					1125:1133	glycan profiling	1118:1133	glycan profiling	1118:1133	Cell lysate of SP cells and main population cells was applied to lectin microarray and mass spectrometry for glycan profiling.
27833472	2	55	theme	SP	378:379	arg1	cells					382:386	so-called side population (SP) cells	351:386	so-called side population (SP) cells	351:386	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	6	56	theme	cell	846:849	arg1	lines					851:855	Six high-grade serous ovarian cancer cell lines	809:855	METHODS Six high-grade serous ovarian cancer cell lines	801:855	METHODS Six high-grade serous ovarian cancer cell lines were used for SP cell isolation.
27833472	2	57	theme	stem	316:319	arg1	properties					331:340	stem cell-like properties	316:340	stem cell-like properties	316:340	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	4	58	theme	glycosylation	605:617	arg1	patterns					619:626	glycosylation patterns	605:626	glycosylation patterns	605:626	Changes in glycosylation patterns are remarkable features of SP cells.
27833472	9	59	theme	lectin	1201:1206	arg1	blot					1208:1211	lectin blot	1201:1211	lectin blot	1201:1211	Differently expressed glycan structures were further verified by lectin blot, flow cytometry, and real-time PCR analysis of their relevant enzymes.
27833472	2	60	theme	cells	305:309	arg1	subpopulations					280:293	subpopulations	280:293	subpopulations	280:293	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	2	60	theme	cells	305:309	arg1	cells					382:386	so-called side population (SP) cells	351:386	so-called side population (SP) cells	351:386	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	6	61	theme	high-grade	813:822	arg1	lines					851:855	Six high-grade serous ovarian cancer cell lines	809:855	METHODS Six high-grade serous ovarian cancer cell lines	801:855	METHODS Six high-grade serous ovarian cancer cell lines were used for SP cell isolation.
27833472	12	62	theme	core	1580:1583	arg1	fucosylation					1585:1596	core fucosylation	1580:1596	core fucosylation	1580:1596	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	5	63	theme	cell	712:715	arg1	lines					717:721	ovarian cancer cell lines	697:721	ovarian cancer cell lines	697:721	Here, we isolated SP cells from ovarian cancer cell lines and analyzed their glycosylation patterns using multiple glycomic strategies.
27833472	4	64	theme	SP	655:656	arg1	cells					658:662	SP cells	655:662	SP cells	655:662	Changes in glycosylation patterns are remarkable features of SP cells.
27833472	1	65	theme	initial	213:219	arg1	treatment					221:229	successful initial treatment	202:229	successful initial treatment	202:229	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	12	66	theme	sT	1563:1564	arg1	antigens					1566:1573	sT antigens	1563:1573	sT antigens	1563:1573	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	9	67	theme	PCR	1244:1246	arg1	analysis					1248:1255	real-time PCR analysis	1234:1255	real-time PCR analysis	1234:1255	Differently expressed glycan structures were further verified by lectin blot, flow cytometry, and real-time PCR analysis of their relevant enzymes.
27833472	2	68	theme	Accumulating	232:243	arg1	evidence					256:263	Accumulating scientific evidence	232:263	Accumulating scientific evidence	232:263	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	1	69	theme	lethal	108:113	arg1	malignancy					129:138	the most lethal gynecological malignancy	99:138	the most lethal gynecological malignancy due to its frequent recurrence and drug resistance	99:189	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	1	69	theme	lethal	108:113	arg1	cancer					89:94	BACKGROUND Ovarian cancer	70:94	BACKGROUND Ovarian cancer	70:94	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	5	70	theme	ovarian	697:703	arg1	lines					717:721	ovarian cancer cell lines	697:721	ovarian cancer cell lines	697:721	Here, we isolated SP cells from ovarian cancer cell lines and analyzed their glycosylation patterns using multiple glycomic strategies.
27833472	11	71	theme	α2,3-linked	1460:1470	arg1	glycan					1479:1484	α2,3-linked sialic glycan	1460:1484	α2,3-linked sialic glycan	1460:1484	By contrast, SP cells exhibited decreased hybrid glycan, α2,3-linked sialic glycan and multivalent sialyl-glycan.
27833472	3	72	theme	new	485:487	arg1	clues					489:493	new clues	485:493	new clues for detecting and targeting these cancer-initiating cells	485:551	A better understanding of SP cells may provide new clues for detecting and targeting these cancer-initiating cells and ultimately help to eradicate cancer.
27833472	5	73	theme	multiple	771:778	arg1	strategies					789:798	multiple glycomic strategies	771:798	multiple glycomic strategies	771:798	Here, we isolated SP cells from ovarian cancer cell lines and analyzed their glycosylation patterns using multiple glycomic strategies.
27833472	9	74	theme	relevant	1266:1273	arg1	enzymes					1275:1281	their relevant enzymes	1260:1281	their relevant enzymes	1260:1281	Differently expressed glycan structures were further verified by lectin blot, flow cytometry, and real-time PCR analysis of their relevant enzymes.
27833472	7	75	theme	SP	999:1000	arg1	cells					1002:1006	SP cells	999:1006	SP cells	999:1006	Among them, HO8910 pm, which contained the highest proportion of SP cells, was used for glycomic analysis of SP cells.
27833472	8	76	theme	SP	1024:1025	arg1	cells					1027:1031	SP cells	1024:1031	SP cells	1024:1031	Cell lysate of SP cells and main population cells was applied to lectin microarray and mass spectrometry for glycan profiling.
27833472	10	77	dep	RESULTS	1284:1290	arg1	Tn					1353:1354	tumor-associated Tn	1336:1354	tumor-associated Tn	1336:1354	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	10	77	dep	RESULTS	1284:1290	arg1	RESULTS					1284:1290	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn	1284:1354	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn	1284:1354	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	10	77	dep	RESULTS	1284:1290	arg1	Expression					1292:1301	Expression	1292:1301	Expression of core fucosylated N-glycan	1292:1330	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	1	78	theme	frequent	151:158	arg1	recurrence					160:169	its frequent recurrence	147:169	its frequent recurrence	147:169	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	11	79	theme	multivalent	1490:1500	arg1	sialyl-glycan					1502:1514	multivalent sialyl-glycan	1490:1514	multivalent sialyl-glycan	1490:1514	By contrast, SP cells exhibited decreased hybrid glycan, α2,3-linked sialic glycan and multivalent sialyl-glycan.
27833472	8	80	theme	population	1042:1051	arg1	cells					1053:1057	main population cells	1037:1057	main population cells	1037:1057	Cell lysate of SP cells and main population cells was applied to lectin microarray and mass spectrometry for glycan profiling.
27833472	1	81	theme	BACKGROUND	70:79	arg1	malignancy					129:138	the most lethal gynecological malignancy	99:138	the most lethal gynecological malignancy due to its frequent recurrence and drug resistance	99:189	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	1	81	theme	BACKGROUND	70:79	arg1	cancer					89:94	BACKGROUND Ovarian cancer	70:94	BACKGROUND Ovarian cancer	70:94	BACKGROUND Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment.
27833472	12	82	theme	ovarian	1625:1631	arg1	cells					1645:1649	ovarian cancer stem cells	1625:1649	ovarian cancer stem cells	1625:1649	CONCLUSIONS Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.
27833472	0	83	theme	glycomic	11:18	arg1	analysis					20:27	glycomic analysis	11:27	glycomic analysis of ovarian cancer side population cells	11:67	Integrated glycomic analysis of ovarian cancer side population cells.
27833472	7	84	theme	cells	958:962	arg1	proportion					941:950	the highest proportion	929:950	the highest proportion of SP cells	929:962	Among them, HO8910 pm, which contained the highest proportion of SP cells, was used for glycomic analysis of SP cells.
27833472	7	85	used	used	969:972	arg2	HO8910 pm					902:910	HO8910 pm	902:910	HO8910 pm	902:910	Among them, HO8910 pm, which contained the highest proportion of SP cells, was used for glycomic analysis of SP cells.
27833472	10	86	theme	N-glycan	1323:1330	arg1	Tn					1353:1354	tumor-associated Tn	1336:1354	tumor-associated Tn	1336:1354	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	10	86	theme	N-glycan	1323:1330	arg1	RESULTS					1284:1290	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn	1284:1354	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn	1284:1354	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	10	86	theme	N-glycan	1323:1330	arg1	Expression					1292:1301	Expression	1292:1301	Expression of core fucosylated N-glycan	1292:1330	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	3	87	theme	better	440:445	arg1	understanding					447:459	A better understanding	438:459	A better understanding of SP cells	438:471	A better understanding of SP cells may provide new clues for detecting and targeting these cancer-initiating cells and ultimately help to eradicate cancer.
27833472	10	88	theme	sT	1363:1364	arg1	RESULTS					1284:1290	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn	1284:1354	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn	1284:1354	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	10	88	theme	sT	1363:1364	arg1	antigens					1366:1373	sT antigens	1363:1373	sT antigens	1363:1373	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	10	89	theme	core	1306:1309	arg1	N-glycan					1323:1330	core fucosylated N-glycan	1306:1330	core fucosylated N-glycan	1306:1330	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	0	90	theme	cancer	40:45	arg1	cells					63:67	ovarian cancer side population cells	32:67	ovarian cancer side population cells	32:67	Integrated glycomic analysis of ovarian cancer side population cells.
27833472	3	91	theme	cells	467:471	arg1	understanding					447:459	A better understanding	438:459	A better understanding of SP cells	438:471	A better understanding of SP cells may provide new clues for detecting and targeting these cancer-initiating cells and ultimately help to eradicate cancer.
27833472	0	92	theme	population	52:61	arg1	cells					63:67	ovarian cancer side population cells	32:67	ovarian cancer side population cells	32:67	Integrated glycomic analysis of ovarian cancer side population cells.
27833472	8	93	theme	mass	1096:1099	arg1	spectrometry					1101:1112	mass spectrometry	1096:1112	mass spectrometry	1096:1112	Cell lysate of SP cells and main population cells was applied to lectin microarray and mass spectrometry for glycan profiling.
27833472	6	94	theme	cell	874:877	arg1	isolation					879:887	SP cell isolation	871:887	SP cell isolation	871:887	METHODS Six high-grade serous ovarian cancer cell lines were used for SP cell isolation.
27833472	10	95	theme	tumor-associated	1336:1351	arg1	Tn					1353:1354	tumor-associated Tn	1336:1354	tumor-associated Tn	1336:1354	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	10	95	theme	tumor-associated	1336:1351	arg1	RESULTS					1284:1290	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn	1284:1354	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn	1284:1354	RESULTS Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells.
27833472	2	96	theme	side	361:364	arg1	cells					382:386	so-called side population (SP) cells	351:386	so-called side population (SP) cells	351:386	Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences.
27833472	4	97	theme	remarkable	632:641	arg1	Changes					594:600	Changes	594:600	Changes in glycosylation patterns	594:626	Changes in glycosylation patterns are remarkable features of SP cells.
27833472	4	97	theme	remarkable	632:641	arg1	features					643:650	remarkable features	632:650	remarkable features of SP cells	632:662	Changes in glycosylation patterns are remarkable features of SP cells.
27833472	9	98	theme	expressed	1148:1156	arg1	structures					1165:1174	Differently expressed glycan structures	1136:1174	Differently expressed glycan structures	1136:1174	Differently expressed glycan structures were further verified by lectin blot, flow cytometry, and real-time PCR analysis of their relevant enzymes.
27833472	3	99	theme	cancer-initiating	529:545	arg1	cells					547:551	these cancer-initiating cells	523:551	these cancer-initiating cells	523:551	A better understanding of SP cells may provide new clues for detecting and targeting these cancer-initiating cells and ultimately help to eradicate cancer.
29022841	8	0	theme	protease	1801:1808	arg1	dressings					1838:1846	protease sequestrant prototype wound dressings	1801:1846	protease sequestrant prototype wound dressings	1801:1846	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	1	1	theme	diagnostics	292:302	arg1	point					278:282	protease point	269:282	protease point of care diagnostics	269:302	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	8	2	theme	prototype	1822:1830	arg1	dressings					1838:1846	protease sequestrant prototype wound dressings	1801:1846	protease sequestrant prototype wound dressings	1801:1846	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	1	3	theme	functional	313:322	arg1	properties					333:342	functional material properties	313:342	functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity	313:420	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	5	4	theme	water	1259:1263	arg1	rates					1284:1288	water vapor transmission rates	1259:1288	water vapor transmission rates	1259:1288	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	6	5	dep	imply	1376:1380	arg1	greater					1453:1459	greater	1453:1459	greater	1453:1459	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	6	5	dep	imply	1376:1380	arg1	greater					1391:1397	greater	1391:1397	greater	1391:1397	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	6	6	theme	zeta	1313:1316	arg1	values					1328:1333	zeta potential values	1313:1333	zeta potential values	1313:1333	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	3	7	with	biosensors	669:678	arg1	surface					713:719	a nanocellulosic transducer surface	685:719	a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid	685:965	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	0	8	theme	material	132:139	arg1	selection					141:149	material selection	132:149	material selection	132:149	Designing cellulosic and nanocellulosic sensors for interface with a protease sequestrant wound-dressing prototype: Implications of material selection for dressing and protease sensor design.
29022841	1	9	theme	chronic	241:247	arg1	dressings					255:263	chronic wound dressings	241:263	chronic wound dressings	241:263	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	4	10	theme	materials	1001:1009	arg1	area					989:992	The specific surface area	968:992	The specific surface area of the materials	968:1009	The specific surface area of the materials correlates with a greater loading of the elastase peptide substrate.
29022841	5	11	theme	vapor	1265:1269	arg1	rates					1284:1288	water vapor transmission rates	1259:1288	water vapor transmission rates	1259:1288	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	1	12	theme	wound	451:455	arg1	environment					457:467	the wound environment	447:467	the wound environment	447:467	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	9	13	theme	chronic	1938:1944	arg1	dressing					1952:1959	current chronic wound dressing design and technology	1930:1981	current chronic wound dressing design and technology	1930:1981	A discussion of the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology is included.
29022841	2	14	theme	design	613:618	arg1	goal					590:593	a goal	588:593	a goal of wound-dressing design for more than a quarter century	588:650	Combining a protease sensor with a dressing is consistent with the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century.
29022841	2	14	theme	design	613:618	arg1	dressing					563:570	an intelligent dressing	548:570	an intelligent dressing	548:570	Combining a protease sensor with a dressing is consistent with the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century.
29022841	3	15	theme	elastase	822:829	arg1	tripeptide					831:840	a fluorescent elastase tripeptide	808:840	a fluorescent elastase tripeptide	808:840	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	7	16	attach	present	1648:1654	arg2	levels					1641:1646	detectable human neutrophil elastase levels	1604:1646	detectable human neutrophil elastase levels present in chronic wound fluid	1604:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	7	16	attach	present	1648:1654	arg1	fluid					1673:1677	chronic wound fluid	1659:1677	chronic wound fluid	1659:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	4	17	theme	surface	981:987	arg1	area					989:992	The specific surface area	968:992	The specific surface area of the materials	968:1009	The specific surface area of the materials correlates with a greater loading of the elastase peptide substrate.
29022841	6	18	theme	sequestration	1362:1374	arg1	degree					1343:1348	the degree	1339:1348	the degree of protease sequestration	1339:1374	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	6	18	theme	sequestration	1362:1374	arg1	correlation					1293:1303	A correlation	1291:1303	A correlation between zeta potential values	1291:1333	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	5	19	theme	gas	1139:1141	arg1	systems					1153:1159	gas permeable systems	1139:1159	gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm)	1139:1227	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	2	20	with	consistent	517:526	arg1	concept					537:543	the concept	533:543	the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century	533:650	Combining a protease sensor with a dressing is consistent with the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century.
29022841	3	21	theme	human	910:914	arg1	elastase					927:934	human neutrophil elastase	910:934	human neutrophil elastase present in chronic wound fluid	910:965	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	2	22	theme	intelligent	551:561	arg1	goal					590:593	a goal	588:593	a goal of wound-dressing design for more than a quarter century	588:650	Combining a protease sensor with a dressing is consistent with the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century.
29022841	2	22	theme	intelligent	551:561	arg1	dressing					563:570	an intelligent dressing	548:570	an intelligent dressing	548:570	Combining a protease sensor with a dressing is consistent with the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century.
29022841	1	23	theme	appropriate	375:385	arg1	charge					395:400	appropriate surface charge	375:400	appropriate surface charge	375:400	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	3	24	theme	wound	955:959	arg1	fluid					961:965	chronic wound fluid	947:965	chronic wound fluid	947:965	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	4	25	theme	elastase	1052:1059	arg1	substrate					1069:1077	the elastase peptide substrate	1048:1077	the elastase peptide substrate	1048:1077	The specific surface area of the materials correlates with a greater loading of the elastase peptide substrate.
29022841	4	26	theme	specific	972:979	arg1	area					989:992	The specific surface area	968:992	The specific surface area of the materials	968:1009	The specific surface area of the materials correlates with a greater loading of the elastase peptide substrate.
29022841	9	27	theme	protease	1882:1889	arg1	sensors					1891:1897	protease sensors	1882:1897	protease sensors	1882:1897	A discussion of the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology is included.
29022841	0	28	theme	sequestrant	78:88	arg1	prototype					105:113	a protease sequestrant wound-dressing prototype	67:113	a protease sequestrant wound-dressing prototype	67:113	Designing cellulosic and nanocellulosic sensors for interface with a protease sequestrant wound-dressing prototype: Implications of material selection for dressing and protease sensor design.
29022841	1	29	theme	charge	395:400	arg1	properties					333:342	functional material properties	313:342	functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity	313:420	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	7	30	theme	wound	1667:1671	arg1	fluid					1673:1677	chronic wound fluid	1659:1677	chronic wound fluid	1659:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	9	31	theme	sensors	1891:1897	arg1	relevance					1869:1877	the relevance	1865:1877	the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology	1865:1981	A discussion of the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology is included.
29022841	4	32	theme	greater	1029:1035	arg1	loading					1037:1043	a greater loading	1027:1043	a greater loading of the elastase peptide substrate	1027:1077	The specific surface area of the materials correlates with a greater loading of the elastase peptide substrate.
29022841	1	33	theme	material	324:331	arg1	properties					333:342	functional material properties	313:342	functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity	313:420	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	6	34	dep	greater	1453:1459	arg1	sequestration					1465:1477	the sequestration	1461:1477	the greater the sequestration of positively charged neutrophil proteases	1449:1520	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	0	35	theme	sensor	177:182	arg1	design					184:189	sensor design	177:189	sensor design	177:189	Designing cellulosic and nanocellulosic sensors for interface with a protease sequestrant wound-dressing prototype: Implications of material selection for dressing and protease sensor design.
29022841	2	36	theme	protease	482:489	arg1	sensor					491:496	a protease sensor	480:496	a protease sensor	480:496	Combining a protease sensor with a dressing is consistent with the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century.
29022841	6	37	theme	neutrophil	1501:1510	arg1	proteases					1512:1520	positively charged neutrophil proteases	1482:1520	positively charged neutrophil proteases	1482:1520	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	7	38	theme	elastase	1632:1639	arg1	levels					1641:1646	detectable human neutrophil elastase levels	1604:1646	detectable human neutrophil elastase levels present in chronic wound fluid	1604:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	4	39	theme	substrate	1069:1077	arg1	loading					1037:1043	a greater loading	1027:1043	a greater loading of the elastase peptide substrate	1027:1077	The specific surface area of the materials correlates with a greater loading of the elastase peptide substrate.
29022841	3	40	theme	nanocellulosic	687:700	arg1	surface					713:719	a nanocellulosic transducer surface	685:719	a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid	685:965	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	1	41	theme	specific	352:359	arg1	area					369:372	high specific surface area	347:372	high specific surface area	347:372	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	7	42	theme	human	1615:1619	arg1	elastase					1632:1639	human neutrophil elastase	1615:1639	detectable human neutrophil elastase levels present in chronic wound fluid	1604:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	1	43	theme	area	369:372	arg1	properties					333:342	functional material properties	313:342	functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity	313:420	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	1	44	with	biosensors	225:234	arg1	dressings					255:263	chronic wound dressings	241:263	chronic wound dressings	241:263	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	5	45	theme	intrusion	1112:1120	arg1	studies					1122:1128	Nitrogen adsorption and mercury intrusion studies	1080:1128	studies	1122:1128	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	8	46	theme	nano-based	1745:1754	arg1	biosensors					1756:1765	the nano-based biosensors	1741:1765	the nano-based biosensors	1741:1765	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	3	47	contain	has	877:879	arg1	tripeptide					831:840	a fluorescent elastase tripeptide	808:840	a fluorescent elastase tripeptide	808:840	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	3	47	contain	has	877:879	arg1	biomolecule					858:868	tetrapeptide biomolecule	845:868	tetrapeptide biomolecule	845:868	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	3	47	contain	has	877:879	arg2	affinity					897:904	affinity	897:904	affinity	897:904	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	3	47	contain	has	877:879	arg2	selectivity					881:891	selectivity	881:891	selectivity	881:891	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	7	48	theme	units/ml	1581:1588	arg1	sensitivities					1553:1565	detection sensitivities	1543:1565	detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid	1543:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	5	49	theme	Nitrogen	1080:1087	arg1	adsorption					1089:1098	Nitrogen adsorption and mercury intrusion studies	1080:1128	adsorption	1089:1098	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	6	50	theme	protease	1353:1360	arg1	sequestration					1362:1374	protease sequestration	1353:1374	protease sequestration	1353:1374	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	1	51	theme	care	287:290	arg1	diagnostics					292:302	care diagnostics	287:302	care diagnostics	287:302	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	5	52	theme	pore	1200:1203	arg1	sizes					1205:1209	pore sizes	1200:1209	pore sizes (2-50 nm, 210 µm)	1200:1227	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	2	53	theme	quarter	636:642	arg1	century					644:650	a quarter century	634:650	a quarter century	634:650	Combining a protease sensor with a dressing is consistent with the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century.
29022841	8	54	theme	biochemical	1715:1725	arg1	properties					1727:1736	the physical and interactive biochemical properties	1686:1736	the physical and interactive biochemical properties of the nano-based biosensors	1686:1765	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	8	54	theme	biochemical	1715:1725	arg1	suitable					1771:1778	suitable	1771:1778	suitable	1771:1778	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	3	55	theme	nanocellulose	736:748	arg1	composites					750:759	nanocellulose composites	736:759	nanocellulose composites	736:759	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	5	56	theme	transmission	1271:1282	arg1	rates					1284:1288	water vapor transmission rates	1259:1288	water vapor transmission rates	1259:1288	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	7	57	theme	detection	1543:1551	arg1	sensitivities					1553:1565	detection sensitivities	1543:1565	detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid	1543:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	5	58	dep	revealed	1130:1137	arg1	influence					1249:1257	influence	1249:1257	influence water vapor transmission rates	1249:1288	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	8	59	theme	sequestrant	1810:1820	arg1	dressings					1838:1846	protease sequestrant prototype wound dressings	1801:1846	protease sequestrant prototype wound dressings	1801:1846	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	9	60	dep	dressing	1952:1959	arg1	design					1961:1966	design	1961:1966	design	1961:1966	A discussion of the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology is included.
29022841	9	60	dep	dressing	1952:1959	arg1	technology					1972:1981	technology	1972:1981	technology	1972:1981	A discussion of the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology is included.
29022841	3	61	from	fluid	961:965	arg1	present					936:942	present	936:942	present	936:942	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	3	62	theme	nanocellulosic	766:779	arg1	aerogels					781:788	nanocellulosic aerogels	766:788	nanocellulosic aerogels	766:788	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	6	63	theme	potential	1318:1326	arg1	values					1328:1333	zeta potential values	1313:1333	zeta potential values	1313:1333	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	8	64	theme	wound	1832:1836	arg1	dressings					1838:1846	protease sequestrant prototype wound dressings	1801:1846	protease sequestrant prototype wound dressings	1801:1846	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	1	65	theme	nanocellulosic-based	204:223	arg1	biosensors					225:234	nanocellulosic-based biosensors	204:234	nanocellulosic-based biosensors with chronic wound dressings	204:263	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	3	66	theme	fluorescent	810:820	arg1	tripeptide					831:840	a fluorescent elastase tripeptide	808:840	a fluorescent elastase tripeptide	808:840	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	6	67	theme	surface	1412:1418	arg1	charge					1420:1425	the negative surface charge	1399:1425	the greater the negative surface charge of the nanomaterials	1387:1446	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	3	68	from	present	936:942	arg1	fluid					961:965	chronic wound fluid	947:965	chronic wound fluid	947:965	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	1	69	theme	wound	249:253	arg1	dressings					255:263	chronic wound dressings	241:263	chronic wound dressings	241:263	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	6	70	dep	greater	1391:1397	arg1	charge					1420:1425	the negative surface charge	1399:1425	the greater the negative surface charge of the nanomaterials	1387:1446	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	3	71	theme	tetrapeptide	845:856	arg1	biomolecule					858:868	tetrapeptide biomolecule	845:868	tetrapeptide biomolecule	845:868	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	0	72	theme	selection	141:149	arg1	Implications					116:127	Implications	116:127	Implications of material selection for dressing and protease sensor design	116:189	Designing cellulosic and nanocellulosic sensors for interface with a protease sequestrant wound-dressing prototype: Implications of material selection for dressing and protease sensor design.
29022841	3	73	attach	present	936:942	arg1	fluid					961:965	chronic wound fluid	947:965	chronic wound fluid	947:965	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	3	73	attach	present	936:942	arg2	elastase					927:934	human neutrophil elastase	910:934	human neutrophil elastase present in chronic wound fluid	910:965	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	3	74	theme	present	936:942	arg1	elastase					927:934	human neutrophil elastase	910:934	human neutrophil elastase present in chronic wound fluid	910:965	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	2	75	theme	wound-dressing	598:611	arg1	design					613:618	wound-dressing design	598:618	wound-dressing design	598:618	Combining a protease sensor with a dressing is consistent with the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century.
29022841	0	76	theme	protease	69:76	arg1	prototype					105:113	a protease sequestrant wound-dressing prototype	67:113	a protease sequestrant wound-dressing prototype	67:113	Designing cellulosic and nanocellulosic sensors for interface with a protease sequestrant wound-dressing prototype: Implications of material selection for dressing and protease sensor design.
29022841	5	77	theme	permeable	1143:1151	arg1	systems					1153:1159	gas permeable systems	1139:1159	gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm)	1139:1227	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	0	78	dep	dressing	155:162	arg1	design					184:189	sensor design	177:189	sensor design	177:189	Designing cellulosic and nanocellulosic sensors for interface with a protease sequestrant wound-dressing prototype: Implications of material selection for dressing and protease sensor design.
29022841	3	79	theme	neutrophil	916:925	arg1	elastase					927:934	human neutrophil elastase	910:934	human neutrophil elastase present in chronic wound fluid	910:965	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	9	80	theme	relevance	1869:1877	arg1	discussion					1851:1860	A discussion	1849:1860	A discussion of the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology	1849:1981	A discussion of the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology is included.
29022841	0	81	theme	wound-dressing	90:103	arg1	prototype					105:113	a protease sequestrant wound-dressing prototype	67:113	a protease sequestrant wound-dressing prototype	67:113	Designing cellulosic and nanocellulosic sensors for interface with a protease sequestrant wound-dressing prototype: Implications of material selection for dressing and protease sensor design.
29022841	1	82	theme	surface	387:393	arg1	charge					395:400	appropriate surface charge	375:400	appropriate surface charge	375:400	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	7	83	theme	chronic	1659:1665	arg1	fluid					1673:1677	chronic wound fluid	1659:1677	chronic wound fluid	1659:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	6	84	theme	negative	1403:1410	arg1	charge					1420:1425	the negative surface charge	1399:1425	the greater the negative surface charge of the nanomaterials	1387:1446	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	3	85	theme	transducer	702:711	arg1	surface					713:719	a nanocellulosic transducer surface	685:719	a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid	685:965	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	3	86	theme	chronic	947:953	arg1	fluid					961:965	chronic wound fluid	947:965	chronic wound fluid	947:965	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	7	87	theme	present	1648:1654	arg1	levels					1641:1646	detectable human neutrophil elastase levels	1604:1646	detectable human neutrophil elastase levels present in chronic wound fluid	1604:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	1	88	theme	hydrophilicity	407:420	arg1	properties					333:342	functional material properties	313:342	functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity	313:420	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	2	89	theme	dressing	563:570	arg1	concept					537:543	the concept	533:543	the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century	533:650	Combining a protease sensor with a dressing is consistent with the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century.
29022841	9	90	theme	cellulose	1903:1911	arg1	nanomaterials					1913:1925	cellulose nanomaterials	1903:1925	cellulose nanomaterials	1903:1925	A discussion of the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology is included.
29022841	0	91	theme	cellulosic	10:19	arg1	sensors					40:46	cellulosic and nanocellulosic sensors	10:46	cellulosic and nanocellulosic sensors for interface	10:60	Designing cellulosic and nanocellulosic sensors for interface with a protease sequestrant wound-dressing prototype: Implications of material selection for dressing and protease sensor design.
29022841	9	92	theme	current	1930:1936	arg1	dressing					1952:1959	current chronic wound dressing design and technology	1930:1981	current chronic wound dressing design and technology	1930:1981	A discussion of the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology is included.
29022841	5	93	dep	sizes	1205:1209	arg1	210 µm					1221:1226	210 µm	1221:1226	210 µm	1221:1226	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	5	93	dep	sizes	1205:1209	arg1	2-50 nm					1212:1218	2-50 nm	1212:1218	2-50 nm	1212:1218	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	0	94	theme	nanocellulosic	25:38	arg1	sensors					40:46	cellulosic and nanocellulosic sensors	10:46	cellulosic and nanocellulosic sensors for interface	10:60	Designing cellulosic and nanocellulosic sensors for interface with a protease sequestrant wound-dressing prototype: Implications of material selection for dressing and protease sensor design.
29022841	7	95	from	present	1648:1654	arg1	fluid					1673:1677	chronic wound fluid	1659:1677	chronic wound fluid	1659:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	9	96	theme	wound	1946:1950	arg1	dressing					1952:1959	current chronic wound dressing design and technology	1930:1981	current chronic wound dressing design and technology	1930:1981	A discussion of the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology is included.
29022841	5	97	with	systems	1153:1159	arg1	%					1193:1193	28-98%	1188:1193	28-98%	1188:1193	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	5	97	with	systems	1153:1159	arg1	sizes					1205:1209	pore sizes	1200:1209	pore sizes (2-50 nm, 210 µm)	1200:1227	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	5	97	with	systems	1153:1159	arg1	porosities					1176:1185	different porosities	1166:1185	different porosities (28-98%)	1166:1194	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	6	98	theme	charged	1493:1499	arg1	proteases					1512:1520	positively charged neutrophil proteases	1482:1520	positively charged neutrophil proteases	1482:1520	A correlation between zeta potential values and the degree of protease sequestration imply that the greater the negative surface charge of the nanomaterials, the greater the sequestration of positively charged neutrophil proteases.
29022841	1	99	theme	high	347:350	arg1	area					369:372	high specific surface area	347:372	high specific surface area	347:372	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	4	100	theme	peptide	1061:1067	arg1	substrate					1069:1077	the elastase peptide substrate	1048:1077	the elastase peptide substrate	1048:1077	The specific surface area of the materials correlates with a greater loading of the elastase peptide substrate.
29022841	9	101	theme	nanomaterials	1913:1925	arg1	relevance					1869:1877	the relevance	1865:1877	the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology	1865:1981	A discussion of the relevance of protease sensors and cellulose nanomaterials to current chronic wound dressing design and technology is included.
29022841	1	102	theme	surface	361:367	arg1	area					369:372	high specific surface area	347:372	high specific surface area	347:372	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	7	103	from	fluid	1673:1677	arg1	present					1648:1654	present	1648:1654	present	1648:1654	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	7	104	theme	neutrophil	1621:1630	arg1	elastase					1632:1639	human neutrophil elastase	1615:1639	detectable human neutrophil elastase levels present in chronic wound fluid	1604:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	5	105	theme	mercury	1104:1110	arg1	studies					1122:1128	Nitrogen adsorption and mercury intrusion studies	1080:1128	studies	1122:1128	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	5	106	theme	different	1166:1174	arg1	%					1193:1193	28-98%	1188:1193	28-98%	1188:1193	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	5	106	theme	different	1166:1174	arg1	porosities					1176:1185	different porosities	1166:1185	different porosities (28-98%)	1166:1194	Nitrogen adsorption and mercury intrusion studies revealed gas permeable systems with different porosities (28-98%) and pore sizes (2-50 nm, 210 µm) respectively, which influence water vapor transmission rates.
29022841	8	107	theme	biosensors	1756:1765	arg1	properties					1727:1736	the physical and interactive biochemical properties	1686:1736	the physical and interactive biochemical properties of the nano-based biosensors	1686:1765	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	8	107	theme	biosensors	1756:1765	arg1	suitable					1771:1778	suitable	1771:1778	suitable	1771:1778	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	0	108	dep	Designing	0:8	arg1	Implications					116:127	Implications	116:127	Implications of material selection for dressing and protease sensor design	116:189	Designing cellulosic and nanocellulosic sensors for interface with a protease sequestrant wound-dressing prototype: Implications of material selection for dressing and protease sensor design.
29022841	7	109	theme	detectable	1604:1613	arg1	levels					1641:1646	detectable human neutrophil elastase levels	1604:1646	detectable human neutrophil elastase levels present in chronic wound fluid	1604:1677	The biosensors gave detection sensitivities of 0.015-0.13 units/ml, which are at detectable human neutrophil elastase levels present in chronic wound fluid.
29022841	8	110	theme	physical	1690:1697	arg1	properties					1727:1736	the physical and interactive biochemical properties	1686:1736	the physical and interactive biochemical properties of the nano-based biosensors	1686:1765	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	8	110	theme	physical	1690:1697	arg1	suitable					1771:1778	suitable	1771:1778	suitable	1771:1778	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	3	111	dep	surface	713:719	arg1	composites					750:759	nanocellulose composites	736:759	nanocellulose composites	736:759	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	3	111	dep	surface	713:719	arg1	aerogels					781:788	nanocellulosic aerogels	766:788	nanocellulosic aerogels	766:788	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	3	111	dep	surface	713:719	arg1	nanocrystals					722:733	nanocrystals	722:733	nanocrystals	722:733	We present here biosensors with a nanocellulosic transducer surface (nanocrystals, nanocellulose composites, and nanocellulosic aerogels) immobilized with a fluorescent elastase tripeptide or tetrapeptide biomolecule, which has selectivity and affinity for human neutrophil elastase present in chronic wound fluid.
29022841	1	112	theme	protease	269:276	arg1	point					278:282	protease point	269:282	protease point of care diagnostics	269:302	Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment.
29022841	8	113	theme	interactive	1703:1713	arg1	properties					1727:1736	the physical and interactive biochemical properties	1686:1736	the physical and interactive biochemical properties of the nano-based biosensors	1686:1765	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
29022841	8	113	theme	interactive	1703:1713	arg1	suitable					1771:1778	suitable	1771:1778	suitable	1771:1778	Thus, the physical and interactive biochemical properties of the nano-based biosensors are suitable for interfacing with protease sequestrant prototype wound dressings.
26002334	0	0	from	materials	19:27	arg1	samples					124:130	honey samples	118:130	honey samples	118:130	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.
26002334	1	1	theme	quinolones	352:361	arg1	concentrations					321:334	the concentrations	317:334	the concentrations of four (fluoro)quinolones (Qs) in honey samples	317:383	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	4	2	dep	conditions	879:888	arg1	sample					896:901	5 mL sample	891:901	5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt	891:1074	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	2	3	with	chromatography	531:544	arg1	detection					570:578	ultraviolet detection	558:578	ultraviolet detection	558:578	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	1	4	theme	bonding	228:234	arg1	β-cyclodextrin					236:249	bonding β-cyclodextrin	228:249	bonding β-cyclodextrin	228:249	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	0	5	theme	measure	77:83	arg1	antibiotics					103:113	measure (fluoro)quinolone antibiotics	77:113	measure (fluoro)quinolone antibiotics	77:113	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.
26002334	1	6	used	used	299:302	arg2	sorbent					141:147	A novel sorbent	133:147	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling	133:293	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	1	6	used	used	299:302	arg2	composite					159:167	β-CD/ATP composite	150:167	β-CD/ATP composite	150:167	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	2	7	dep	Qs	423:424	arg1	ofloxacin					455:463	ofloxacin	455:463	ofloxacin	455:463	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	2	7	dep	Qs	423:424	arg1	gatifloxacin					470:481	gatifloxacin	470:481	gatifloxacin	470:481	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	2	7	dep	Qs	423:424	arg1	norfloxacin					442:452	norfloxacin	442:452	norfloxacin	442:452	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	2	7	dep	Qs	423:424	arg1	ciprofloxacin					427:439	ciprofloxacin	427:439	ciprofloxacin	427:439	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	4	8	theme	salt	1071:1074	arg1	addition					1059:1066	no addition	1056:1066	no addition of salt	1056:1074	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	8	theme	salt	1071:1074	arg1	mg					914:915	4 mg	912:915	4 mg of β-CD/ATP composite as the sorbent	912:952	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	8	theme	salt	1071:1074	arg1	μL					959:960	200 μL	955:960	200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min	955:1049	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	8	theme	salt	1071:1074	arg1	pH					906:907	pH 3	906:909	pH 3	906:909	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	3	9	theme	sample	793:798	arg1	pH					783:784	pH	783:784	pH of the sample	783:798	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	9	theme	sample	793:798	arg1	eluent					695:700	the eluent	691:700	the eluent	691:700	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	9	theme	sample	793:798	arg1	sorbent					726:732	the sorbent	722:732	the sorbent	722:732	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	9	theme	sample	793:798	arg1	type					672:675	type	672:675	type	672:675	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	9	theme	sample	793:798	arg1	process					770:776	the vortex and sonication process	744:776	process	770:776	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	9	theme	sample	793:798	arg1	type					703:706	type	703:706	type	703:706	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	9	theme	sample	793:798	arg1	sample					793:798	the sample	789:798	the sample	789:798	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	9	theme	sample	793:798	arg1	times					735:739	times	735:739	times of the vortex and sonication process	735:776	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	9	theme	sample	793:798	arg1	amount					712:717	amount	712:717	amount	712:717	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	9	theme	sample	793:798	arg1	volume					681:686	volume	681:686	volume	681:686	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	8	10	from	palygorskite	1671:1682	arg1	applications					1698:1709	analytical applications	1687:1709	analytical applications	1687:1709	Although the LODs were slightly higher than expected, this study confirmed the possibility of using cyclodextrin grafted palygorskite in analytical applications.
26002334	2	11	theme	ultraviolet	558:568	arg1	detection					570:578	ultraviolet detection	558:578	ultraviolet detection	558:578	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	6	12	theme	standard	1303:1310	arg1	deviations					1312:1321	the relative standard deviations	1290:1321	the relative standard deviations (RSDs)	1290:1328	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	6	12	theme	standard	1303:1310	arg1	%					1393:1393	less than 7.4%	1380:1393	less than 7.4%	1380:1393	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	6	12	theme	standard	1303:1310	arg1	RSDs					1324:1327	RSDs	1324:1327	RSDs	1324:1327	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	0	13	theme	fluoro	86:91	arg1	antibiotics					103:113	measure (fluoro)quinolone antibiotics	77:113	measure (fluoro)quinolone antibiotics	77:113	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.
26002334	4	14	theme	β-CD/ATP	920:927	arg1	addition					1059:1066	no addition	1056:1066	no addition of salt	1056:1074	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	14	theme	β-CD/ATP	920:927	arg1	mg					914:915	4 mg	912:915	4 mg of β-CD/ATP composite as the sorbent	912:952	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	14	theme	β-CD/ATP	920:927	arg1	μL					959:960	200 μL	955:960	200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min	955:1049	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	14	theme	β-CD/ATP	920:927	arg1	pH					906:907	pH 3	906:909	pH 3	906:909	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	7	15	theme	honey	1437:1441	arg1	samples					1443:1449	three real honey samples	1426:1449	three real honey samples	1426:1449	The method was validated with three real honey samples, and the results demonstrated that β-CD/ATP composite possessed a high adsorption capacity for Qs.
26002334	1	16	theme	modified	254:261	arg1	attapulgite					263:273	modified attapulgite	254:273	modified attapulgite	254:273	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	4	17	theme	5	891:891	arg1	mL					893:894	mL	893:894	mL	893:894	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	3	18	theme	eluent	695:700	arg1	pH					783:784	pH	783:784	pH of the sample	783:798	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	18	theme	eluent	695:700	arg1	eluent					695:700	the eluent	691:700	the eluent	691:700	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	18	theme	eluent	695:700	arg1	sorbent					726:732	the sorbent	722:732	the sorbent	722:732	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	18	theme	eluent	695:700	arg1	type					672:675	type	672:675	type	672:675	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	18	theme	eluent	695:700	arg1	process					770:776	the vortex and sonication process	744:776	process	770:776	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	18	theme	eluent	695:700	arg1	type					703:706	type	703:706	type	703:706	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	18	theme	eluent	695:700	arg1	sample					793:798	the sample	789:798	the sample	789:798	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	18	theme	eluent	695:700	arg1	times					735:739	times	735:739	times of the vortex and sonication process	735:776	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	18	theme	eluent	695:700	arg1	amount					712:717	amount	712:717	amount	712:717	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	18	theme	eluent	695:700	arg1	volume					681:686	volume	681:686	volume	681:686	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	5	19	from	L	1206:1206	arg1	range					1179:1183	the range	1175:1183	the range from 0.30 to 3.95 μg L(-1)	1175:1210	The limits of detection (LODs) were determined to the range from 0.30 to 3.95 μg L(-1).
26002334	4	20	with	sonication	1029:1038	arg1	60s					1021:1023	vortex time 60s	1009:1023	vortex time 60s	1009:1023	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	3	21	theme	column	835:840	arg1	procedures					842:851	batch and column procedures	825:851	procedures	842:851	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	1	22	theme	β-CD/ATP	150:157	arg1	sorbent					141:147	A novel sorbent	133:147	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling	133:293	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	1	22	theme	β-CD/ATP	150:157	arg1	composite					159:167	β-CD/ATP composite	150:167	β-CD/ATP composite	150:167	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	0	23	theme	quinolone	93:101	arg1	antibiotics					103:113	measure (fluoro)quinolone antibiotics	77:113	measure (fluoro)quinolone antibiotics	77:113	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.
26002334	3	24	theme	process	770:776	arg1	pH					783:784	pH	783:784	pH of the sample	783:798	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	24	theme	process	770:776	arg1	eluent					695:700	the eluent	691:700	the eluent	691:700	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	24	theme	process	770:776	arg1	sorbent					726:732	the sorbent	722:732	the sorbent	722:732	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	24	theme	process	770:776	arg1	type					672:675	type	672:675	type	672:675	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	24	theme	process	770:776	arg1	process					770:776	the vortex and sonication process	744:776	process	770:776	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	24	theme	process	770:776	arg1	type					703:706	type	703:706	type	703:706	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	24	theme	process	770:776	arg1	sample					793:798	the sample	789:798	the sample	789:798	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	24	theme	process	770:776	arg1	times					735:739	times	735:739	times of the vortex and sonication process	735:776	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	24	theme	process	770:776	arg1	amount					712:717	amount	712:717	amount	712:717	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	24	theme	process	770:776	arg1	volume					681:686	volume	681:686	volume	681:686	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	7	25	theme	high	1517:1520	arg1	capacity					1533:1540	a high adsorption capacity	1515:1540	a high adsorption capacity for Qs	1515:1547	The method was validated with three real honey samples, and the results demonstrated that β-CD/ATP composite possessed a high adsorption capacity for Qs.
26002334	1	26	from	quinolones	352:361	arg1	samples					377:383	honey samples	371:383	honey samples	371:383	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	0	27	theme	composite	9:17	arg1	materials					19:27	β-CD/ATP composite materials	0:27	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.	0:131	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.
26002334	0	28	from	use	33:35	arg1	extraction					63:72	dispersive solid-phase extraction	40:72	dispersive solid-phase extraction	40:72	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.
26002334	2	29	theme	d-SPE	590:594	arg1	procedure					596:604	the d-SPE procedure	586:604	the d-SPE procedure	586:604	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	7	30	theme	adsorption	1522:1531	arg1	capacity					1533:1540	a high adsorption capacity	1515:1540	a high adsorption capacity for Qs	1515:1547	The method was validated with three real honey samples, and the results demonstrated that β-CD/ATP composite possessed a high adsorption capacity for Qs.
26002334	4	31	from	addition	1059:1066	arg1	methanol					980:987	methanol	980:987	methanol as the eluent, with vortex time 60s and sonication time 6 min	980:1049	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	31	from	addition	1059:1066	arg1	sample					896:901	5 mL sample	891:901	5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt	891:1074	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	3	32	theme	batch	825:829	arg1	procedures					842:851	batch and column procedures	825:851	procedures	842:851	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	0	33	theme	β-CD/ATP	0:7	arg1	materials					19:27	β-CD/ATP composite materials	0:27	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.	0:131	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.
26002334	4	34	with	eluent	996:1001	arg1	60s					1021:1023	vortex time 60s	1009:1023	vortex time 60s	1009:1023	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	0	35	theme	dispersive	40:49	arg1	extraction					63:72	dispersive solid-phase extraction	40:72	dispersive solid-phase extraction	40:72	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.
26002334	8	36	theme	grafted	1663:1669	arg1	palygorskite					1671:1682	cyclodextrin grafted palygorskite	1650:1682	cyclodextrin grafted palygorskite in analytical applications	1650:1709	Although the LODs were slightly higher than expected, this study confirmed the possibility of using cyclodextrin grafted palygorskite in analytical applications.
26002334	0	37	theme	honey	118:122	arg1	samples					124:130	honey samples	118:130	honey samples	118:130	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.
26002334	3	38	theme	sonication	759:768	arg1	process					770:776	the vortex and sonication process	744:776	process	770:776	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	4	39	theme	ammonia	969:975	arg1	addition					1059:1066	no addition	1056:1066	no addition of salt	1056:1074	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	39	theme	ammonia	969:975	arg1	mg					914:915	4 mg	912:915	4 mg of β-CD/ATP composite as the sorbent	912:952	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	39	theme	ammonia	969:975	arg1	μL					959:960	200 μL	955:960	200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min	955:1049	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	39	theme	ammonia	969:975	arg1	pH					906:907	pH 3	906:909	pH 3	906:909	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	1	40	theme	dispersive	174:183	arg1	d-SPE					209:213	d-SPE	209:213	d-SPE	209:213	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	1	40	theme	dispersive	174:183	arg1	extraction					197:206	dispersive solid-phase extraction	174:206	dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin	174:249	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	2	41	theme	subsequent	390:399	arg1	quantification					401:414	The subsequent quantification	386:414	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin)	386:482	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	1	42	theme	silane	279:284	arg1	coupling					286:293	silane coupling	279:293	silane coupling	279:293	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	2	43	theme	high-performance	507:522	arg1	HPLC					547:550	HPLC	547:550	HPLC	547:550	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	2	43	theme	high-performance	507:522	arg1	chromatography					531:544	high-performance liquid chromatography	507:544	high-performance liquid chromatography (HPLC) with ultraviolet detection	507:578	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	1	44	theme	solid-phase	185:195	arg1	d-SPE					209:213	d-SPE	209:213	d-SPE	209:213	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	1	44	theme	solid-phase	185:195	arg1	extraction					197:206	dispersive solid-phase extraction	174:206	dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin	174:249	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	4	45	theme	optimal	858:864	arg1	conditions					879:888	The optimal experimental conditions	854:888	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt)	854:1075	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	8	46	theme	analytical	1687:1696	arg1	applications					1698:1709	analytical applications	1687:1709	analytical applications	1687:1709	Although the LODs were slightly higher than expected, this study confirmed the possibility of using cyclodextrin grafted palygorskite in analytical applications.
26002334	6	47	theme	relative	1294:1301	arg1	deviations					1312:1321	the relative standard deviations	1290:1321	the relative standard deviations (RSDs)	1290:1328	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	6	47	theme	relative	1294:1301	arg1	%					1393:1393	less than 7.4%	1380:1393	less than 7.4%	1380:1393	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	6	47	theme	relative	1294:1301	arg1	RSDs					1324:1327	RSDs	1324:1327	RSDs	1324:1327	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	3	48	theme	sorbent	726:732	arg1	pH					783:784	pH	783:784	pH of the sample	783:798	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	48	theme	sorbent	726:732	arg1	eluent					695:700	the eluent	691:700	the eluent	691:700	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	48	theme	sorbent	726:732	arg1	sorbent					726:732	the sorbent	722:732	the sorbent	722:732	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	48	theme	sorbent	726:732	arg1	type					672:675	type	672:675	type	672:675	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	48	theme	sorbent	726:732	arg1	process					770:776	the vortex and sonication process	744:776	process	770:776	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	48	theme	sorbent	726:732	arg1	type					703:706	type	703:706	type	703:706	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	48	theme	sorbent	726:732	arg1	sample					793:798	the sample	789:798	the sample	789:798	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	48	theme	sorbent	726:732	arg1	times					735:739	times	735:739	times of the vortex and sonication process	735:776	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	48	theme	sorbent	726:732	arg1	amount					712:717	amount	712:717	amount	712:717	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	3	48	theme	sorbent	726:732	arg1	volume					681:686	volume	681:686	volume	681:686	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	4	49	theme	%	967:967	arg1	ammonia					969:975	40% ammonia	965:975	40% ammonia	965:975	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	50	theme	time	1040:1043	arg1	min					1047:1049	time 6 min	1040:1049	time 6 min	1040:1049	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	5	51	dep	3.95	1198:1201	arg1	to					1195:1196	to	1195:1196	to	1195:1196	The limits of detection (LODs) were determined to the range from 0.30 to 3.95 μg L(-1).
26002334	0	52	theme	solid-phase	51:61	arg1	extraction					63:72	dispersive solid-phase extraction	40:72	dispersive solid-phase extraction	40:72	β-CD/ATP composite materials for use in dispersive solid-phase extraction to measure (fluoro)quinolone antibiotics in honey samples.
26002334	4	53	from	mg	914:915	arg1	methanol					980:987	methanol	980:987	methanol as the eluent, with vortex time 60s and sonication time 6 min	980:1049	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	53	from	mg	914:915	arg1	sample					896:901	5 mL sample	891:901	5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt	891:1074	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	7	54	contain	possessed	1505:1513	arg2	capacity					1533:1540	a high adsorption capacity	1515:1540	a high adsorption capacity for Qs	1515:1547	The method was validated with three real honey samples, and the results demonstrated that β-CD/ATP composite possessed a high adsorption capacity for Qs.
26002334	7	54	contain	possessed	1505:1513	arg1	composite					1495:1503	β-CD/ATP composite	1486:1503	β-CD/ATP composite	1486:1503	The method was validated with three real honey samples, and the results demonstrated that β-CD/ATP composite possessed a high adsorption capacity for Qs.
26002334	1	55	from	concentrations	321:334	arg1	samples					377:383	honey samples	371:383	honey samples	371:383	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	7	56	theme	real	1432:1435	arg1	samples					1443:1449	three real honey samples	1426:1449	three real honey samples	1426:1449	The method was validated with three real honey samples, and the results demonstrated that β-CD/ATP composite possessed a high adsorption capacity for Qs.
26002334	2	57	theme	liquid	524:529	arg1	HPLC					547:550	HPLC	547:550	HPLC	547:550	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	2	57	theme	liquid	524:529	arg1	chromatography					531:544	high-performance liquid chromatography	507:544	high-performance liquid chromatography (HPLC) with ultraviolet detection	507:578	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	1	58	theme	honey	371:375	arg1	samples					377:383	honey samples	371:383	honey samples	371:383	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	5	59	theme	μg	1203:1204	arg1	L					1206:1206	0.30 to 3.95 μg L(-1)	1190:1210	0.30 to 3.95 μg L(-1)	1190:1210	The limits of detection (LODs) were determined to the range from 0.30 to 3.95 μg L(-1).
26002334	5	60	theme	detection	1139:1147	arg1	limits					1129:1134	The limits	1125:1134	The limits of detection (LODs)	1125:1154	The limits of detection (LODs) were determined to the range from 0.30 to 3.95 μg L(-1).
26002334	7	61	theme	β-CD/ATP	1486:1493	arg1	composite					1495:1503	β-CD/ATP composite	1486:1503	β-CD/ATP composite	1486:1503	The method was validated with three real honey samples, and the results demonstrated that β-CD/ATP composite possessed a high adsorption capacity for Qs.
26002334	6	62	theme	optimum	1266:1272	arg1	conditions					1274:1283	the optimum conditions	1262:1283	the optimum conditions	1262:1283	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	4	63	dep	eluent	996:1001	arg1	min					1047:1049	time 6 min	1040:1049	time 6 min	1040:1049	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	64	from	pH	906:907	arg1	methanol					980:987	methanol	980:987	methanol as the eluent, with vortex time 60s and sonication time 6 min	980:1049	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	64	from	pH	906:907	arg1	sample					896:901	5 mL sample	891:901	5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt	891:1074	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	65	theme	statistical	1101:1111	arg1	evaluation					1113:1122	this statistical evaluation	1096:1122	this statistical evaluation	1096:1122	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	66	theme	time	1016:1019	arg1	60s					1021:1023	vortex time 60s	1009:1023	vortex time 60s	1009:1023	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	67	from	μL	959:960	arg1	methanol					980:987	methanol	980:987	methanol as the eluent, with vortex time 60s and sonication time 6 min	980:1049	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	67	from	μL	959:960	arg1	sample					896:901	5 mL sample	891:901	5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt	891:1074	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	2	68	theme	Qs	423:424	arg1	quantification					401:414	The subsequent quantification	386:414	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin)	386:482	The subsequent quantification of the Qs (ciprofloxacin, norfloxacin, ofloxacin, and gatifloxacin) was accomplished using high-performance liquid chromatography (HPLC) with ultraviolet detection after the d-SPE procedure.
26002334	3	69	theme	vortex	748:753	arg1	process					770:776	the vortex and sonication process	744:776	process	770:776	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	8	70	theme	cyclodextrin	1650:1661	arg1	palygorskite					1671:1682	cyclodextrin grafted palygorskite	1650:1682	cyclodextrin grafted palygorskite in analytical applications	1650:1709	Although the LODs were slightly higher than expected, this study confirmed the possibility of using cyclodextrin grafted palygorskite in analytical applications.
26002334	3	71	theme	extraction	641:650	arg1	efficiency					652:661	the extraction efficiency	637:661	the extraction efficiency	637:661	Parameters that may influence the extraction efficiency, such as type and volume of the eluent, type and amount of the sorbent, times of the vortex and sonication process, and pH of the sample, were investigated using batch and column procedures.
26002334	4	72	theme	vortex	1009:1014	arg1	60s					1021:1023	vortex time 60s	1009:1023	vortex time 60s	1009:1023	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	6	73	used	used	1341:1344	arg2	RSDs					1324:1327	RSDs	1324:1327	RSDs	1324:1327	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	6	73	used	used	1341:1344	arg2	deviations					1312:1321	the relative standard deviations	1290:1321	the relative standard deviations (RSDs)	1290:1328	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	6	73	used	used	1341:1344	arg2	%					1393:1393	less than 7.4%	1380:1393	less than 7.4%	1380:1393	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	4	74	theme	40	965:966	arg1	%					967:967	%	967:967	%	967:967	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	4	75	theme	mL	893:894	arg1	sample					896:901	5 mL sample	891:901	5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt	891:1074	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
26002334	6	76	theme	Good	1213:1216	arg1	recoveries					1218:1227	Good recoveries	1213:1227	Good recoveries (83.6-88.6%)	1213:1240	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	6	76	theme	Good	1213:1216	arg1	%					1239:1239	83.6-88.6%	1230:1239	83.6-88.6%	1230:1239	Good recoveries (83.6-88.6%) were obtained under the optimum conditions, and the relative standard deviations (RSDs), which are used to indicate reproducibility, were less than 7.4%.
26002334	1	77	from	samples	377:383	arg1	concentrations					321:334	the concentrations	317:334	the concentrations of four (fluoro)quinolones (Qs) in honey samples	317:383	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	1	78	theme	novel	135:139	arg1	sorbent					141:147	A novel sorbent	133:147	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling	133:293	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	1	78	theme	novel	135:139	arg1	composite					159:167	β-CD/ATP composite	150:167	β-CD/ATP composite	150:167	A novel sorbent (β-CD/ATP composite) for dispersive solid-phase extraction (d-SPE) prepared by bonding β-cyclodextrin to modified attapulgite via silane coupling was used to determine the concentrations of four (fluoro)quinolones (Qs) in honey samples.
26002334	4	79	theme	experimental	866:877	arg1	conditions					879:888	The optimal experimental conditions	854:888	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt)	854:1075	The optimal experimental conditions (5 mL sample at pH 3, 4 mg of β-CD/ATP composite as the sorbent, 200 μL of 40% ammonia in methanol as the eluent, with vortex time 60s and sonication time 6 min, and no addition of salt) were obtained from this statistical evaluation.
27138966	0	0	theme	pH	107:108	arg1	effect					97:102	effect	97:102	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.	0:170	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
27138966	1	1	from	effect	387:392	arg1	properties					458:467	their rheological and swelling properties	427:467	their rheological and swelling properties	427:467	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	2	2	theme	degradation	570:580	arg1	rate					582:585	the thermal degradation rate	558:585	the thermal degradation rate	558:585	The semi-IPNs are thermally stable up to about 400 °C and the presence of CHI increases the thermal degradation rate compared to bare poly (NIPAAm).
27138966	1	3	theme	free	265:268	arg1	polymerization					278:291	free radical polymerization	265:291	free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)]	265:332	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	2	4	theme	thermal	562:568	arg1	rate					582:585	the thermal degradation rate	558:585	the thermal degradation rate	558:585	The semi-IPNs are thermally stable up to about 400 °C and the presence of CHI increases the thermal degradation rate compared to bare poly (NIPAAm).
27138966	1	5	theme	temperature	404:414	arg1	changes					416:422	pH and temperature changes	397:422	pH and temperature changes	397:422	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	1	6	theme	radical	270:276	arg1	polymerization					278:291	free radical polymerization	265:291	free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)]	265:332	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	0	7	theme	temperature	114:124	arg1	effect					97:102	effect	97:102	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.	0:170	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
27138966	1	8	theme	chitosan	354:361	arg1	presence					342:349	the presence	338:349	the presence of chitosan (CHI)	338:367	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	1	9	theme	changes	416:422	arg1	effect					387:392	the effect	383:392	the effect of pH and temperature changes on their rheological and swelling properties	383:467	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	7	10	theme	bare	1429:1432	arg1	NIPAAm					1440:1445	NIPAAm	1440:1445	NIPAAm	1440:1445	Moreover, the semi-IPNs viscoelastic moduli increase with the increase of CHI content and, in particular, the difference between the elastic modulus before and after the sol/gel transition is higher for the semi-IPN than for bare poly (NIPAAm) just at about physiological conditions.
27138966	7	10	theme	bare	1429:1432	arg1	poly					1434:1437	bare poly	1429:1437	bare poly (NIPAAm)	1429:1446	Moreover, the semi-IPNs viscoelastic moduli increase with the increase of CHI content and, in particular, the difference between the elastic modulus before and after the sol/gel transition is higher for the semi-IPN than for bare poly (NIPAAm) just at about physiological conditions.
27138966	5	11	dep	approaches	1062:1071	arg1	that					1073:1076	that	1073:1076	that	1073:1076	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	3	12	theme	appropriate	761:771	arg1	support					773:779	appropriate support	761:779	appropriate support for cell proliferation	761:802	The prepared systems presents a well-defined porosity and proved to be non-toxic, in vitro, on human embryonic skin fibroblast, thus offering appropriate support for cell proliferation.
27138966	7	13	theme	elastic	1337:1343	arg1	modulus					1345:1351	the elastic modulus	1333:1351	the elastic modulus before and after the sol/gel transition	1333:1391	Moreover, the semi-IPNs viscoelastic moduli increase with the increase of CHI content and, in particular, the difference between the elastic modulus before and after the sol/gel transition is higher for the semi-IPN than for bare poly (NIPAAm) just at about physiological conditions.
27138966	2	14	theme	bare	599:602	arg1	NIPAAm					610:615	NIPAAm	610:615	NIPAAm	610:615	The semi-IPNs are thermally stable up to about 400 °C and the presence of CHI increases the thermal degradation rate compared to bare poly (NIPAAm).
27138966	2	14	theme	bare	599:602	arg1	poly					604:607	bare poly	599:607	bare poly (NIPAAm)	599:616	The semi-IPNs are thermally stable up to about 400 °C and the presence of CHI increases the thermal degradation rate compared to bare poly (NIPAAm).
27138966	7	15	theme	semi-IPNs	1218:1226	arg1	moduli					1241:1246	the semi-IPNs viscoelastic moduli	1214:1246	the semi-IPNs viscoelastic moduli	1214:1246	Moreover, the semi-IPNs viscoelastic moduli increase with the increase of CHI content and, in particular, the difference between the elastic modulus before and after the sol/gel transition is higher for the semi-IPN than for bare poly (NIPAAm) just at about physiological conditions.
27138966	2	16	dep	°C	521:522	arg1	up					505:506	up	505:506	up	505:506	The semi-IPNs are thermally stable up to about 400 °C and the presence of CHI increases the thermal degradation rate compared to bare poly (NIPAAm).
27138966	5	17	dep	degree	1050:1055	arg1	approaches					1062:1071	approaches	1062:1071	approaches that of bare poly (NIPAAm)	1062:1098	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	5	18	theme	water	1026:1030	arg1	diffusion					1013:1021	the diffusion	1009:1021	the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm)	1009:1098	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	1	19	theme	rheological	433:443	arg1	properties					458:467	their rheological and swelling properties	427:467	their rheological and swelling properties	427:467	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	1	20	theme	work	188:191	arg1	aim					176:178	The aim	172:178	The aim of this work	172:191	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	0	21	theme	chitosan/poly	19:31	arg1	networks					87:94	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks	0:94	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.	0:170	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
27138966	0	22	theme	Stimuli-responsive	0:17	arg1	networks					87:94	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks	0:94	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.	0:170	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
27138966	1	23	theme	N-isopropylacrylamide	296:316	arg1	NIPAAm					325:330	NIPAAm	325:330	NIPAAm	325:330	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	1	23	theme	N-isopropylacrylamide	296:316	arg1	[poly					318:322	N-isopropylacrylamide [poly	296:322	N-isopropylacrylamide [poly (NIPAAm)]	296:332	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	6	24	theme	semi-IPNs	1132:1140	arg1	moduli					1118:1123	The viscoelastic moduli	1101:1123	The viscoelastic moduli of the semi-IPNs	1101:1140	The viscoelastic moduli of the semi-IPNs increase as a function of pH while the LCST remain unchanged.
27138966	1	25	theme	swelling	449:456	arg1	properties					458:467	their rheological and swelling properties	427:467	their rheological and swelling properties	427:467	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	6	26	theme	pH	1168:1169	arg1	function					1156:1163	a function	1154:1163	a function of pH	1154:1169	The viscoelastic moduli of the semi-IPNs increase as a function of pH while the LCST remain unchanged.
27138966	0	27	theme	N-isopropylacrylamide	34:54	arg1	networks					87:94	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks	0:94	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.	0:170	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
27138966	1	28	theme	[poly	318:322	arg1	polymerization					278:291	free radical polymerization	265:291	free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)]	265:332	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	6	29	theme	viscoelastic	1105:1116	arg1	moduli					1118:1123	The viscoelastic moduli	1101:1123	The viscoelastic moduli of the semi-IPNs	1101:1140	The viscoelastic moduli of the semi-IPNs increase as a function of pH while the LCST remain unchanged.
27138966	7	30	theme	CHI	1278:1280	arg1	content					1282:1288	CHI content	1278:1288	CHI content	1278:1288	Moreover, the semi-IPNs viscoelastic moduli increase with the increase of CHI content and, in particular, the difference between the elastic modulus before and after the sol/gel transition is higher for the semi-IPN than for bare poly (NIPAAm) just at about physiological conditions.
27138966	5	31	theme	poly	1086:1089	arg1	approaches					1062:1071	approaches	1062:1071	approaches that of bare poly (NIPAAm)	1062:1098	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	0	32	theme	rheological	135:145	arg1	properties					160:169	their rheological and swelling properties	129:169	their rheological and swelling properties	129:169	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
27138966	7	33	theme	content	1282:1288	arg1	increase					1266:1273	the increase	1262:1273	the increase of CHI content	1262:1288	Moreover, the semi-IPNs viscoelastic moduli increase with the increase of CHI content and, in particular, the difference between the elastic modulus before and after the sol/gel transition is higher for the semi-IPN than for bare poly (NIPAAm) just at about physiological conditions.
27138966	5	34	from	pH	933:934	arg1	protonated					964:973	protonated	964:973	protonated	964:973	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	5	34	from	pH	933:934	arg1	macromolecules					945:958	the CHI macromolecules	937:958	the CHI macromolecules	937:958	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	1	35	theme	semi-interpenetrating	211:231	arg1	semi-IPNs					251:259	semi-IPNs	251:259	semi-IPNs	251:259	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	1	35	theme	semi-interpenetrating	211:231	arg1	networks					241:248	semi-interpenetrating polymer networks	211:248	semi-interpenetrating polymer networks (semi-IPNs)	211:260	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	0	36	theme	semi-interpenetrating	57:77	arg1	networks					87:94	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks	0:94	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.	0:170	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
27138966	0	37	dep	networks	87:94	arg1	effect					97:102	effect	97:102	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.	0:170	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
27138966	1	38	theme	polymer	233:239	arg1	semi-IPNs					251:259	semi-IPNs	251:259	semi-IPNs	251:259	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	1	38	theme	polymer	233:239	arg1	networks					241:248	semi-interpenetrating polymer networks	211:248	semi-interpenetrating polymer networks (semi-IPNs)	211:260	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	3	39	theme	human	714:718	arg1	fibroblast					735:744	human embryonic skin fibroblast	714:744	human embryonic skin fibroblast	714:744	The prepared systems presents a well-defined porosity and proved to be non-toxic, in vitro, on human embryonic skin fibroblast, thus offering appropriate support for cell proliferation.
27138966	0	40	theme	swelling	151:158	arg1	properties					160:169	their rheological and swelling properties	129:169	their rheological and swelling properties	129:169	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
27138966	0	41	from	effect	97:102	arg1	properties					160:169	their rheological and swelling properties	129:169	their rheological and swelling properties	129:169	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
27138966	3	42	attach	presents	640:647	arg2	systems					632:638	The prepared systems	619:638	The prepared systems	619:638	The prepared systems presents a well-defined porosity and proved to be non-toxic, in vitro, on human embryonic skin fibroblast, thus offering appropriate support for cell proliferation.
27138966	3	42	attach	presents	640:647	arg2	non-toxic					690:698	non-toxic	690:698	non-toxic	690:698	The prepared systems presents a well-defined porosity and proved to be non-toxic, in vitro, on human embryonic skin fibroblast, thus offering appropriate support for cell proliferation.
27138966	3	42	attach	presents	640:647	arg1	fibroblast					735:744	human embryonic skin fibroblast	714:744	human embryonic skin fibroblast	714:744	The prepared systems presents a well-defined porosity and proved to be non-toxic, in vitro, on human embryonic skin fibroblast, thus offering appropriate support for cell proliferation.
27138966	7	43	theme	physiological	1462:1474	arg1	conditions					1476:1485	about physiological conditions	1456:1485	about physiological conditions	1456:1485	Moreover, the semi-IPNs viscoelastic moduli increase with the increase of CHI content and, in particular, the difference between the elastic modulus before and after the sol/gel transition is higher for the semi-IPN than for bare poly (NIPAAm) just at about physiological conditions.
27138966	3	44	theme	embryonic	720:728	arg1	fibroblast					735:744	human embryonic skin fibroblast	714:744	human embryonic skin fibroblast	714:744	The prepared systems presents a well-defined porosity and proved to be non-toxic, in vitro, on human embryonic skin fibroblast, thus offering appropriate support for cell proliferation.
27138966	2	45	theme	CHI	544:546	arg1	presence					532:539	the presence	528:539	the presence of CHI	528:546	The semi-IPNs are thermally stable up to about 400 °C and the presence of CHI increases the thermal degradation rate compared to bare poly (NIPAAm).
27138966	5	46	theme	bare	1081:1084	arg1	NIPAAm					1092:1097	NIPAAm	1092:1097	NIPAAm	1092:1097	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	5	46	theme	bare	1081:1084	arg1	poly					1086:1089	bare poly	1081:1089	bare poly (NIPAAm)	1081:1098	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	0	47	theme	polymer	79:85	arg1	networks					87:94	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks	0:94	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.	0:170	Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
27138966	4	48	theme	physiological	831:843	arg1	pH					845:846	physiological pH	831:846	physiological pH	831:846	The semi-IPNs present, at physiological pH, swelling degrees well below those of the pure poly (NIPAAm).
27138966	3	49	theme	prepared	623:630	arg1	non-toxic					690:698	non-toxic	690:698	non-toxic	690:698	The prepared systems presents a well-defined porosity and proved to be non-toxic, in vitro, on human embryonic skin fibroblast, thus offering appropriate support for cell proliferation.
27138966	3	49	theme	prepared	623:630	arg1	systems					632:638	The prepared systems	619:638	The prepared systems	619:638	The prepared systems presents a well-defined porosity and proved to be non-toxic, in vitro, on human embryonic skin fibroblast, thus offering appropriate support for cell proliferation.
27138966	5	50	theme	CHI	941:943	arg1	protonated					964:973	protonated	964:973	protonated	964:973	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	5	50	theme	CHI	941:943	arg1	macromolecules					945:958	the CHI macromolecules	937:958	the CHI macromolecules	937:958	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	7	51	theme	viscoelastic	1228:1239	arg1	moduli					1241:1246	the semi-IPNs viscoelastic moduli	1214:1246	the semi-IPNs viscoelastic moduli	1214:1246	Moreover, the semi-IPNs viscoelastic moduli increase with the increase of CHI content and, in particular, the difference between the elastic modulus before and after the sol/gel transition is higher for the semi-IPN than for bare poly (NIPAAm) just at about physiological conditions.
27138966	4	52	theme	swelling	849:856	arg1	degrees					858:864	swelling degrees	849:864	swelling degrees well below those of the pure poly (NIPAAm)	849:907	The semi-IPNs present, at physiological pH, swelling degrees well below those of the pure poly (NIPAAm).
27138966	4	53	attach	present	819:825	arg2	semi-IPNs					809:817	The semi-IPNs	805:817	The semi-IPNs	805:817	The semi-IPNs present, at physiological pH, swelling degrees well below those of the pure poly (NIPAAm).
27138966	4	53	attach	present	819:825	arg1	pH					845:846	physiological pH	831:846	physiological pH	831:846	The semi-IPNs present, at physiological pH, swelling degrees well below those of the pure poly (NIPAAm).
27138966	1	54	theme	pH	397:398	arg1	changes					416:422	pH and temperature changes	397:422	pH and temperature changes	397:422	The aim of this work was to synthesize semi-interpenetrating polymer networks (semi-IPNs) by free radical polymerization of N-isopropylacrylamide [poly (NIPAAm)], in the presence of chitosan (CHI), and to study the effect of pH and temperature changes on their rheological and swelling properties.
27138966	3	55	theme	cell	785:788	arg1	proliferation					790:802	cell proliferation	785:802	cell proliferation	785:802	The prepared systems presents a well-defined porosity and proved to be non-toxic, in vitro, on human embryonic skin fibroblast, thus offering appropriate support for cell proliferation.
27138966	7	56	theme	sol/gel	1374:1380	arg1	transition					1382:1391	the sol/gel transition	1370:1391	the sol/gel transition	1370:1391	Moreover, the semi-IPNs viscoelastic moduli increase with the increase of CHI content and, in particular, the difference between the elastic modulus before and after the sol/gel transition is higher for the semi-IPN than for bare poly (NIPAAm) just at about physiological conditions.
27138966	5	57	theme	swelling	1041:1048	arg1	degree					1050:1055	a swelling degree	1039:1055	a swelling degree that approaches that of bare poly (NIPAAm)	1039:1098	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	3	58	theme	skin	730:733	arg1	fibroblast					735:744	human embryonic skin fibroblast	714:744	human embryonic skin fibroblast	714:744	The prepared systems presents a well-defined porosity and proved to be non-toxic, in vitro, on human embryonic skin fibroblast, thus offering appropriate support for cell proliferation.
27138966	3	59	theme	well-defined	651:662	arg1	porosity					664:671	a well-defined porosity	649:671	a well-defined porosity	649:671	The prepared systems presents a well-defined porosity and proved to be non-toxic, in vitro, on human embryonic skin fibroblast, thus offering appropriate support for cell proliferation.
27138966	5	60	theme	acidic	926:931	arg1	pH					933:934	acidic pH	926:934	acidic pH	926:934	Differently, at acidic pH, the CHI macromolecules are protonated and become much more permeable to the diffusion of water giving a swelling degree that approaches that of bare poly (NIPAAm).
27138966	4	61	theme	pure	890:893	arg1	NIPAAm					901:906	NIPAAm	901:906	NIPAAm	901:906	The semi-IPNs present, at physiological pH, swelling degrees well below those of the pure poly (NIPAAm).
27138966	4	61	theme	pure	890:893	arg1	poly					895:898	the pure poly	886:898	the pure poly (NIPAAm)	886:907	The semi-IPNs present, at physiological pH, swelling degrees well below those of the pure poly (NIPAAm).
24504748	8	0	theme	dual-delivery	1391:1403	arg1	alternative					1429:1439	an attractive alternative	1415:1439	an attractive alternative to single delivery or preloaded systems for bone regeneration	1415:1501	This dual-delivery system is an attractive alternative to single delivery or preloaded systems for bone regeneration since it can simultaneously fight infection and deliver a potent growth factor.
24504748	8	0	theme	dual-delivery	1391:1403	arg1	system					1405:1410	This dual-delivery system	1386:1410	This dual-delivery system	1386:1410	This dual-delivery system is an attractive alternative to single delivery or preloaded systems for bone regeneration since it can simultaneously fight infection and deliver a potent growth factor.
24504748	6	1	theme	calcium-based	1202:1214	arg1	materials					1216:1224	calcium-based materials	1202:1224	calcium-based materials	1202:1224	Released rhBMP-2 levels were, however, too low to induce detectable levels of ALP in W20-17 cells, due to the affinity of rhBMP-2 for calcium-based materials.
24504748	1	2	theme	bone	159:162	arg1	graft					164:168	A biodegradable, composite bone graft	132:168	A biodegradable, composite bone graft	132:168	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	0	3	theme	vancomycin	69:78	arg1	delivery					57:64	dual delivery	52:64	dual delivery of vancomycin and recombinant human bone morphogenetic protein-2	52:129	Composite chitosan and calcium sulfate scaffold for dual delivery of vancomycin and recombinant human bone morphogenetic protein-2.
24504748	6	4	theme	rhBMP-2	1077:1083	arg1	levels					1085:1090	Released rhBMP-2 levels	1068:1090	Released rhBMP-2 levels	1068:1090	Released rhBMP-2 levels were, however, too low to induce detectable levels of ALP in W20-17 cells, due to the affinity of rhBMP-2 for calcium-based materials.
24504748	0	5	theme	human	96:100	arg1	protein-2					121:129	recombinant human bone morphogenetic protein-2	84:129	recombinant human bone morphogenetic protein-2	84:129	Composite chitosan and calcium sulfate scaffold for dual delivery of vancomycin and recombinant human bone morphogenetic protein-2.
24504748	3	6	theme	drugs	706:710	arg1	induction					638:646	induction	638:646	induction of ALP expression by released rhBMP-2	638:684	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	6	theme	drugs	706:710	arg1	vancomycin					626:635	released vancomycin	617:635	released vancomycin	617:635	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	6	theme	drugs	706:710	arg1	interaction					691:701	interaction	691:701	interaction of drugs on cells	691:719	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	6	7	theme	rhBMP-2	1190:1196	arg1	affinity					1178:1185	the affinity	1174:1185	the affinity of rhBMP-2 for calcium-based materials	1174:1224	Released rhBMP-2 levels were, however, too low to induce detectable levels of ALP in W20-17 cells, due to the affinity of rhBMP-2 for calcium-based materials.
24504748	6	8	theme	W20-17	1153:1158	arg1	cells					1160:1164	W20-17 cells	1153:1164	W20-17 cells	1153:1164	Released rhBMP-2 levels were, however, too low to induce detectable levels of ALP in W20-17 cells, due to the affinity of rhBMP-2 for calcium-based materials.
24504748	9	9	theme	functional	1808:1817	arg1	activity					1819:1826	functional activity	1808:1826	functional activity	1808:1826	Additionally, this composite can accommodate a wide range of therapeutics and thus be customizable for specific patient needs, however, the potential interactive effects of multiple agents must be investigated to ensure that functional activity is not altered.
24504748	0	10	theme	recombinant	84:94	arg1	protein-2					121:129	recombinant human bone morphogenetic protein-2	84:129	recombinant human bone morphogenetic protein-2	84:129	Composite chitosan and calcium sulfate scaffold for dual delivery of vancomycin and recombinant human bone morphogenetic protein-2.
24504748	3	11	theme	drug	550:553	arg1	kinetics					563:570	drug release kinetics	550:570	drug release kinetics	550:570	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	1	12	dep	biodegradable	134:146	arg1	composite					149:157	composite	149:157	composite	149:157	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	3	13	from	vancomycin	626:635	arg1	cells					715:719	cells	715:719	cells	715:719	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	1	14	theme	point-of-care	261:273	arg1	loading					275:281	point-of-care loading	261:281	point-of-care loading	261:281	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	0	15	theme	morphogenetic	107:119	arg1	protein-2					121:129	recombinant human bone morphogenetic protein-2	84:129	recombinant human bone morphogenetic protein-2	84:129	Composite chitosan and calcium sulfate scaffold for dual delivery of vancomycin and recombinant human bone morphogenetic protein-2.
24504748	8	16	theme	attractive	1418:1427	arg1	alternative					1429:1439	an attractive alternative	1415:1439	an attractive alternative to single delivery or preloaded systems for bone regeneration	1415:1501	This dual-delivery system is an attractive alternative to single delivery or preloaded systems for bone regeneration since it can simultaneously fight infection and deliver a potent growth factor.
24504748	8	16	theme	attractive	1418:1427	arg1	system					1405:1410	This dual-delivery system	1386:1410	This dual-delivery system	1386:1410	This dual-delivery system is an attractive alternative to single delivery or preloaded systems for bone regeneration since it can simultaneously fight infection and deliver a potent growth factor.
24504748	4	17	theme	>MIC	839:842	arg1	μg/mL					849:853	>MIC 8-16 μg/mL	839:853	>MIC 8-16 μg/mL	839:853	Results showed the composite set in under 36 min and released vancomycin levels that were bactericidal to S. aureus (>MIC 8-16 μg/mL) for 18 days.
24504748	4	17	theme	>MIC	839:842	arg1	aureus					831:836	S. aureus	828:836	S. aureus (>MIC 8-16 μg/mL)	828:854	Results showed the composite set in under 36 min and released vancomycin levels that were bactericidal to S. aureus (>MIC 8-16 μg/mL) for 18 days.
24504748	7	18	theme	stimulating	1232:1242	arg1	rhBMP-2					1255:1261	rhBMP-2	1255:1261	rhBMP-2 (>50 ng/mL)	1255:1273	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	7	18	theme	stimulating	1232:1242	arg1	ng/mL					1268:1272	>50 ng/mL	1264:1272	>50 ng/mL	1264:1272	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	7	18	theme	stimulating	1232:1242	arg1	amounts					1244:1250	stimulating amounts	1232:1250	stimulating amounts of rhBMP-2 (>50 ng/mL)	1232:1273	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	3	19	theme	set	540:542	arg1	time					544:547	set time	540:547	set time	540:547	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	9	20	theme	potential	1723:1731	arg1	effects					1745:1751	the potential interactive effects	1719:1751	the potential interactive effects of multiple agents	1719:1770	Additionally, this composite can accommodate a wide range of therapeutics and thus be customizable for specific patient needs, however, the potential interactive effects of multiple agents must be investigated to ensure that functional activity is not altered.
24504748	7	21	theme	rhBMP-2	1255:1261	arg1	rhBMP-2					1255:1261	rhBMP-2	1255:1261	rhBMP-2 (>50 ng/mL)	1255:1273	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	7	21	theme	rhBMP-2	1255:1261	arg1	ng/mL					1268:1272	>50 ng/mL	1264:1272	>50 ng/mL	1264:1272	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	7	21	theme	rhBMP-2	1255:1261	arg1	amounts					1244:1250	stimulating amounts	1232:1250	stimulating amounts of rhBMP-2 (>50 ng/mL)	1232:1273	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	0	22	theme	bone	102:105	arg1	protein-2					121:129	recombinant human bone morphogenetic protein-2	84:129	recombinant human bone morphogenetic protein-2	84:129	Composite chitosan and calcium sulfate scaffold for dual delivery of vancomycin and recombinant human bone morphogenetic protein-2.
24504748	5	23	theme	initial	1051:1057	arg1	loading					1059:1065	initial loading	1051:1065	initial loading	1051:1065	Composites exhibited a 1 day-delayed release, followed by a continuous release of rhBMP-2 over 6 weeks; ranging from 0.06 to 1.49 ng/mL, and showed a dose dependent release based on initial loading.
24504748	3	24	from	induction	638:646	arg1	cells					715:719	cells	715:719	cells	715:719	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	5	25	dep	1.49	994:997	arg1	to					991:992	to	991:992	to	991:992	Composites exhibited a 1 day-delayed release, followed by a continuous release of rhBMP-2 over 6 weeks; ranging from 0.06 to 1.49 ng/mL, and showed a dose dependent release based on initial loading.
24504748	9	26	theme	specific	1686:1693	arg1	patient					1695:1701	specific patient needs	1686:1707	specific patient needs	1686:1707	Additionally, this composite can accommodate a wide range of therapeutics and thus be customizable for specific patient needs, however, the potential interactive effects of multiple agents must be investigated to ensure that functional activity is not altered.
24504748	1	27	theme	large	376:380	arg1	injuries					387:394	large bone injuries	376:394	large bone injuries	376:394	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	9	28	theme	therapeutics	1644:1655	arg1	range					1635:1639	a wide range	1628:1639	a wide range of therapeutics	1628:1655	Additionally, this composite can accommodate a wide range of therapeutics and thus be customizable for specific patient needs, however, the potential interactive effects of multiple agents must be investigated to ensure that functional activity is not altered.
24504748	8	29	theme	potent	1561:1566	arg1	factor					1575:1580	a potent growth factor	1559:1580	a potent growth factor	1559:1580	This dual-delivery system is an attractive alternative to single delivery or preloaded systems for bone regeneration since it can simultaneously fight infection and deliver a potent growth factor.
24504748	0	30	theme	Composite	0:8	arg1	chitosan					10:17	Composite chitosan	0:17	Composite chitosan	0:17	Composite chitosan and calcium sulfate scaffold for dual delivery of vancomycin and recombinant human bone morphogenetic protein-2.
24504748	9	31	theme	wide	1630:1633	arg1	range					1635:1639	a wide range	1628:1639	a wide range of therapeutics	1628:1655	Additionally, this composite can accommodate a wide range of therapeutics and thus be customizable for specific patient needs, however, the potential interactive effects of multiple agents must be investigated to ensure that functional activity is not altered.
24504748	4	32	from	set	751:753	arg1	min					767:769	under 36 min	758:769	under 36 min	758:769	Results showed the composite set in under 36 min and released vancomycin levels that were bactericidal to S. aureus (>MIC 8-16 μg/mL) for 18 days.
24504748	4	33	theme	composite	741:749	arg1	set					751:753	the composite set	737:753	the composite set in under 36 min	737:769	Results showed the composite set in under 36 min and released vancomycin levels that were bactericidal to S. aureus (>MIC 8-16 μg/mL) for 18 days.
24504748	1	34	theme	chitosan	183:190	arg1	microspheres					192:203	chitosan microspheres	183:203	chitosan microspheres embedded in calcium sulfate	183:231	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	0	35	theme	calcium	23:29	arg1	sulfate					31:37	calcium sulfate	23:37	calcium sulfate	23:37	Composite chitosan and calcium sulfate scaffold for dual delivery of vancomycin and recombinant human bone morphogenetic protein-2.
24504748	3	36	theme	release	555:561	arg1	kinetics					563:570	drug release kinetics	550:570	drug release kinetics	550:570	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	9	37	theme	multiple	1756:1763	arg1	agents					1765:1770	multiple agents	1756:1770	multiple agents	1756:1770	Additionally, this composite can accommodate a wide range of therapeutics and thus be customizable for specific patient needs, however, the potential interactive effects of multiple agents must be investigated to ensure that functional activity is not altered.
24504748	1	38	from	healing	365:371	arg1	injuries					387:394	large bone injuries	376:394	large bone injuries	376:394	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	0	39	theme	protein-2	121:129	arg1	delivery					57:64	dual delivery	52:64	dual delivery of vancomycin and recombinant human bone morphogenetic protein-2	52:129	Composite chitosan and calcium sulfate scaffold for dual delivery of vancomycin and recombinant human bone morphogenetic protein-2.
24504748	1	40	theme	bone	382:385	arg1	injuries					387:394	large bone injuries	376:394	large bone injuries	376:394	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	5	41	theme	continuous	929:938	arg1	release					940:946	a continuous release	927:946	a continuous release of rhBMP-2	927:957	Composites exhibited a 1 day-delayed release, followed by a continuous release of rhBMP-2 over 6 weeks; ranging from 0.06 to 1.49 ng/mL, and showed a dose dependent release based on initial loading.
24504748	8	42	theme	single	1444:1449	arg1	delivery					1451:1458	single delivery	1444:1458	single delivery	1444:1458	This dual-delivery system is an attractive alternative to single delivery or preloaded systems for bone regeneration since it can simultaneously fight infection and deliver a potent growth factor.
24504748	1	43	theme	embedded	205:212	arg1	microspheres					192:203	chitosan microspheres	183:203	chitosan microspheres embedded in calcium sulfate	183:231	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	9	44	theme	interactive	1733:1743	arg1	effects					1745:1751	the potential interactive effects	1719:1751	the potential interactive effects of multiple agents	1719:1770	Additionally, this composite can accommodate a wide range of therapeutics and thus be customizable for specific patient needs, however, the potential interactive effects of multiple agents must be investigated to ensure that functional activity is not altered.
24504748	5	45	theme	day-delayed	894:904	arg1	release					906:912	a 1 day-delayed release	890:912	a 1 day-delayed release	890:912	Composites exhibited a 1 day-delayed release, followed by a continuous release of rhBMP-2 over 6 weeks; ranging from 0.06 to 1.49 ng/mL, and showed a dose dependent release based on initial loading.
24504748	3	46	from	interaction	691:701	arg1	cells					715:719	cells	715:719	cells	715:719	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	47	theme	released	669:676	arg1	rhBMP-2					678:684	released rhBMP-2	669:684	released rhBMP-2	669:684	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	7	48	theme	high	1342:1345	arg1	levels					1358:1363	high vancomycin levels	1342:1363	high vancomycin levels (1,800-3,600 μg/mL)	1342:1383	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	7	48	theme	high	1342:1345	arg1	μg/mL					1378:1382	1,800-3,600 μg/mL	1366:1382	1,800-3,600 μg/mL	1366:1382	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	2	49	theme	calcium	470:476	arg1	sulfate					478:484	calcium sulfate	470:484	calcium sulfate loaded with vancomycin	470:507	Microspheres were loaded with rhBMP-2 or vancomycin prior to mixing into calcium sulfate loaded with vancomycin.
24504748	1	50	theme	antibiotics	299:309	arg1	delivery					287:294	delivery	287:294	delivery	287:294	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	1	50	theme	antibiotics	299:309	arg1	loading					275:281	point-of-care loading	261:281	point-of-care loading	261:281	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	3	51	from	drugs	706:710	arg1	cells					715:719	cells	715:719	cells	715:719	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	52	theme	ALP	651:653	arg1	expression					655:664	ALP expression	651:664	ALP expression	651:664	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	53	theme	released	617:624	arg1	vancomycin					626:635	released vancomycin	617:635	released vancomycin	617:635	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	1	54	theme	calcium	217:223	arg1	sulfate					225:231	calcium sulfate	217:231	calcium sulfate	217:231	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	0	55	theme	dual	52:55	arg1	delivery					57:64	dual delivery	52:64	dual delivery of vancomycin and recombinant human bone morphogenetic protein-2	52:129	Composite chitosan and calcium sulfate scaffold for dual delivery of vancomycin and recombinant human bone morphogenetic protein-2.
24504748	8	56	theme	bone	1485:1488	arg1	regeneration					1490:1501	bone regeneration	1485:1501	bone regeneration	1485:1501	This dual-delivery system is an attractive alternative to single delivery or preloaded systems for bone regeneration since it can simultaneously fight infection and deliver a potent growth factor.
24504748	3	57	theme	expression	655:664	arg1	induction					638:646	induction	638:646	induction of ALP expression by released rhBMP-2	638:684	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	57	theme	expression	655:664	arg1	vancomycin					626:635	released vancomycin	617:635	released vancomycin	617:635	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	57	theme	expression	655:664	arg1	interaction					691:701	interaction	691:701	interaction of drugs on cells	691:719	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	58	theme	bacteriostatic/bactericidal	577:603	arg1	activity					605:612	bacteriostatic/bactericidal activity	577:612	bacteriostatic/bactericidal activity	577:612	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	7	59	theme	vancomycin	1347:1356	arg1	levels					1358:1363	high vancomycin levels	1342:1363	high vancomycin levels (1,800-3,600 μg/mL)	1342:1383	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	7	59	theme	vancomycin	1347:1356	arg1	μg/mL					1378:1382	1,800-3,600 μg/mL	1366:1382	1,800-3,600 μg/mL	1366:1382	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	3	60	from	cells	715:719	arg1	induction					638:646	induction	638:646	induction of ALP expression by released rhBMP-2	638:684	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	60	from	cells	715:719	arg1	vancomycin					626:635	released vancomycin	617:635	released vancomycin	617:635	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	60	from	cells	715:719	arg1	interaction					691:701	interaction	691:701	interaction of drugs on cells	691:719	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	1	61	theme	growth	315:320	arg1	factors					322:328	growth factors	315:328	growth factors	315:328	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	8	62	theme	preloaded	1463:1471	arg1	systems					1473:1479	preloaded systems	1463:1479	preloaded systems	1463:1479	This dual-delivery system is an attractive alternative to single delivery or preloaded systems for bone regeneration since it can simultaneously fight infection and deliver a potent growth factor.
24504748	7	63	theme	ALP	1280:1282	arg1	response					1284:1291	the ALP response	1276:1291	the ALP response from W-20-17 cells	1276:1310	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	5	64	theme	dependent	1024:1032	arg1	release					1034:1040	a dose dependent release	1017:1040	a dose dependent release based on initial loading	1017:1065	Composites exhibited a 1 day-delayed release, followed by a continuous release of rhBMP-2 over 6 weeks; ranging from 0.06 to 1.49 ng/mL, and showed a dose dependent release based on initial loading.
24504748	8	65	theme	growth	1568:1573	arg1	factor					1575:1580	a potent growth factor	1559:1580	a potent growth factor	1559:1580	This dual-delivery system is an attractive alternative to single delivery or preloaded systems for bone regeneration since it can simultaneously fight infection and deliver a potent growth factor.
24504748	1	66	theme	factors	322:328	arg1	delivery					287:294	delivery	287:294	delivery	287:294	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	1	66	theme	factors	322:328	arg1	loading					275:281	point-of-care loading	261:281	point-of-care loading	261:281	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	1	67	from	sulfate	225:231	arg1	embedded					205:212	embedded	205:212	embedded	205:212	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	3	68	theme	interaction	691:701	arg1	kinetics					563:570	drug release kinetics	550:570	drug release kinetics	550:570	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	68	theme	interaction	691:701	arg1	time					544:547	set time	540:547	set time	540:547	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	68	theme	interaction	691:701	arg1	activity					605:612	bacteriostatic/bactericidal activity	577:612	bacteriostatic/bactericidal activity	577:612	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	69	theme	induction	638:646	arg1	kinetics					563:570	drug release kinetics	550:570	drug release kinetics	550:570	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	69	theme	induction	638:646	arg1	time					544:547	set time	540:547	set time	540:547	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	69	theme	induction	638:646	arg1	activity					605:612	bacteriostatic/bactericidal activity	577:612	bacteriostatic/bactericidal activity	577:612	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	1	70	from	embedded	205:212	arg1	sulfate					225:231	calcium sulfate	217:231	calcium sulfate	217:231	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	6	71	theme	Released	1068:1075	arg1	levels					1085:1090	Released rhBMP-2 levels	1068:1090	Released rhBMP-2 levels	1068:1090	Released rhBMP-2 levels were, however, too low to induce detectable levels of ALP in W20-17 cells, due to the affinity of rhBMP-2 for calcium-based materials.
24504748	7	72	theme	W-20-17	1298:1304	arg1	cells					1306:1310	W-20-17 cells	1298:1310	W-20-17 cells	1298:1310	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	9	73	dep	patient	1695:1701	arg1	needs					1703:1707	needs	1703:1707	needs	1703:1707	Additionally, this composite can accommodate a wide range of therapeutics and thus be customizable for specific patient needs, however, the potential interactive effects of multiple agents must be investigated to ensure that functional activity is not altered.
24504748	4	74	theme	vancomycin	784:793	arg1	levels					795:800	vancomycin levels	784:800	vancomycin levels that were bactericidal to S. aureus (>MIC 8-16 μg/mL) for 18 days	784:866	Results showed the composite set in under 36 min and released vancomycin levels that were bactericidal to S. aureus (>MIC 8-16 μg/mL) for 18 days.
24504748	1	75	theme	biodegradable	134:146	arg1	graft					164:168	A biodegradable, composite bone graft	132:168	A biodegradable, composite bone graft	132:168	A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries.
24504748	5	76	theme	rhBMP-2	951:957	arg1	release					940:946	a continuous release	927:946	a continuous release of rhBMP-2	927:957	Composites exhibited a 1 day-delayed release, followed by a continuous release of rhBMP-2 over 6 weeks; ranging from 0.06 to 1.49 ng/mL, and showed a dose dependent release based on initial loading.
24504748	3	77	theme	vancomycin	626:635	arg1	kinetics					563:570	drug release kinetics	550:570	drug release kinetics	550:570	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	77	theme	vancomycin	626:635	arg1	time					544:547	set time	540:547	set time	540:547	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	3	77	theme	vancomycin	626:635	arg1	activity					605:612	bacteriostatic/bactericidal activity	577:612	bacteriostatic/bactericidal activity	577:612	Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells.
24504748	7	78	from	cells	1306:1310	arg1	response					1284:1291	the ALP response	1276:1291	the ALP response from W-20-17 cells	1276:1310	With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800-3,600 μg/mL).
24504748	8	79	theme	fight	1531:1535	arg1	infection					1537:1545	fight infection	1531:1545	fight infection	1531:1545	This dual-delivery system is an attractive alternative to single delivery or preloaded systems for bone regeneration since it can simultaneously fight infection and deliver a potent growth factor.
24504748	6	80	theme	detectable	1125:1134	arg1	levels					1136:1141	detectable levels	1125:1141	detectable levels of ALP	1125:1148	Released rhBMP-2 levels were, however, too low to induce detectable levels of ALP in W20-17 cells, due to the affinity of rhBMP-2 for calcium-based materials.
24504748	9	81	theme	agents	1765:1770	arg1	effects					1745:1751	the potential interactive effects	1719:1751	the potential interactive effects of multiple agents	1719:1770	Additionally, this composite can accommodate a wide range of therapeutics and thus be customizable for specific patient needs, however, the potential interactive effects of multiple agents must be investigated to ensure that functional activity is not altered.
24504748	6	82	theme	ALP	1146:1148	arg1	levels					1136:1141	detectable levels	1125:1141	detectable levels of ALP	1125:1148	Released rhBMP-2 levels were, however, too low to induce detectable levels of ALP in W20-17 cells, due to the affinity of rhBMP-2 for calcium-based materials.
29198147	3	0	theme	filtered	696:703	arg1	solvent					705:711	the filtered solvent	692:711	the filtered solvent	692:711	Electrospray ionisation mass spectrometry was used to confirm the formation of the thymol/β-CD complex and gas chromatography was used to quantify the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent.
29198147	4	1	theme	reported	850:857	arg1	methods					859:865	previously reported methods	839:865	previously reported methods that have been applied to other agents	839:904	The systematic optimisation of the conditions improved both the yield of the complex and the IE of the AM agents compared to previously reported methods that have been applied to other agents.
29198147	2	2	theme	total	367:371	arg1	volume					381:386	total solvent volume	367:386	total solvent volume	367:386	Conditions including solvent composition, temperature, reaction time and total solvent volume were investigated to optimise the inclusion efficiency (IE) and yield.
29198147	3	3	theme	complex	554:560	arg1	formation					525:533	the formation	521:533	the formation of the thymol/β-CD complex	521:560	Electrospray ionisation mass spectrometry was used to confirm the formation of the thymol/β-CD complex and gas chromatography was used to quantify the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent.
29198147	1	4	with	complexes	153:161	arg1	agents					205:210	naturally derived antimicrobial (AM) agents	168:210	naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool	168:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	4	with	complexes	153:161	arg1	linalool					235:242	linalool	235:242	linalool	235:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	4	with	complexes	153:161	arg1	AM					201:202	AM	201:202	AM	201:202	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	4	with	complexes	153:161	arg1	carvacrol					221:229	carvacrol	221:229	carvacrol	221:229	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	4	with	complexes	153:161	arg1	thymol					213:218	thymol	213:218	thymol	213:218	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	5	5	theme	AM	937:938	arg1	agent					940:944	the AM agent	933:944	the AM agent	933:944	Using a 1:1 mole ratio of the AM agent to β-CD, the optimised parameters resulted in maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent.
29198147	4	6	theme	agents	820:825	arg1	IE					807:808	the IE	803:808	the IE of the AM agents	803:825	The systematic optimisation of the conditions improved both the yield of the complex and the IE of the AM agents compared to previously reported methods that have been applied to other agents.
29198147	4	6	theme	agents	820:825	arg1	yield					778:782	the yield	774:782	the yield of the complex	774:797	The systematic optimisation of the conditions improved both the yield of the complex and the IE of the AM agents compared to previously reported methods that have been applied to other agents.
29198147	5	7	theme	agent	940:944	arg1	ratio					924:928	a 1:1 mole ratio	913:928	a 1:1 mole ratio of the AM agent to β-CD	913:952	Using a 1:1 mole ratio of the AM agent to β-CD, the optimised parameters resulted in maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent.
29198147	1	8	theme	derived	178:184	arg1	agents					205:210	naturally derived antimicrobial (AM) agents	168:210	naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool	168:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	8	theme	derived	178:184	arg1	linalool					235:242	linalool	235:242	linalool	235:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	8	theme	derived	178:184	arg1	AM					201:202	AM	201:202	AM	201:202	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	8	theme	derived	178:184	arg1	carvacrol					221:229	carvacrol	221:229	carvacrol	221:229	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	8	theme	derived	178:184	arg1	thymol					213:218	thymol	213:218	thymol	213:218	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	4	9	theme	AM	817:818	arg1	agents					820:825	the AM agents	813:825	the AM agents	813:825	The systematic optimisation of the conditions improved both the yield of the complex and the IE of the AM agents compared to previously reported methods that have been applied to other agents.
29198147	3	10	theme	thymol/β-CD	542:552	arg1	complex					554:560	the thymol/β-CD complex	538:560	the thymol/β-CD complex	538:560	Electrospray ionisation mass spectrometry was used to confirm the formation of the thymol/β-CD complex and gas chromatography was used to quantify the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent.
29198147	1	11	theme	antimicrobial	186:198	arg1	agents					205:210	naturally derived antimicrobial (AM) agents	168:210	naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool	168:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	11	theme	antimicrobial	186:198	arg1	linalool					235:242	linalool	235:242	linalool	235:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	11	theme	antimicrobial	186:198	arg1	AM					201:202	AM	201:202	AM	201:202	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	11	theme	antimicrobial	186:198	arg1	carvacrol					221:229	carvacrol	221:229	carvacrol	221:229	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	11	theme	antimicrobial	186:198	arg1	thymol					213:218	thymol	213:218	thymol	213:218	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	12	link	derived	178:184	arg1	agents					205:210	naturally derived antimicrobial (AM) agents	168:210	naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool	168:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	12	link	derived	178:184	arg1	linalool					235:242	linalool	235:242	linalool	235:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	12	link	derived	178:184	arg1	AM					201:202	AM	201:202	AM	201:202	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	12	link	derived	178:184	arg1	carvacrol					221:229	carvacrol	221:229	carvacrol	221:229	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	12	link	derived	178:184	arg1	thymol					213:218	thymol	213:218	thymol	213:218	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	4	13	theme	conditions	749:758	arg1	optimisation					729:740	The systematic optimisation	714:740	The systematic optimisation of the conditions	714:758	The systematic optimisation of the conditions improved both the yield of the complex and the IE of the AM agents compared to previously reported methods that have been applied to other agents.
29198147	1	14	theme	co-precipitation	266:281	arg1	technique					283:291	a co-precipitation technique	264:291	a co-precipitation technique	264:291	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	5	15	theme	1:1 mole	915:922	arg1	ratio					924:928	a 1:1 mole ratio	913:928	a 1:1 mole ratio of the AM agent to β-CD	913:952	Using a 1:1 mole ratio of the AM agent to β-CD, the optimised parameters resulted in maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent.
29198147	0	16	theme	inclusion	31:39	arg1	complexes					41:49	β-cyclodextrin inclusion complexes	16:49	β-cyclodextrin inclusion complexes	16:49	Optimisation of β-cyclodextrin inclusion complexes with natural antimicrobial agents: thymol, carvacrol and linalool.
29198147	5	17	theme	maximum	992:998	arg1	yields					1000:1005	maximum yields	992:1005	maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent	992:1124	Using a 1:1 mole ratio of the AM agent to β-CD, the optimised parameters resulted in maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent.
29198147	2	18	theme	solvent	315:321	arg1	composition					323:333	solvent composition	315:333	solvent composition	315:333	Conditions including solvent composition, temperature, reaction time and total solvent volume were investigated to optimise the inclusion efficiency (IE) and yield.
29198147	3	19	theme	ionisation	472:481	arg1	spectrometry					488:499	Electrospray ionisation mass spectrometry	459:499	Electrospray ionisation mass spectrometry	459:499	Electrospray ionisation mass spectrometry was used to confirm the formation of the thymol/β-CD complex and gas chromatography was used to quantify the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent.
29198147	0	20	theme	β-cyclodextrin	16:29	arg1	complexes					41:49	β-cyclodextrin inclusion complexes	16:49	β-cyclodextrin inclusion complexes	16:49	Optimisation of β-cyclodextrin inclusion complexes with natural antimicrobial agents: thymol, carvacrol and linalool.
29198147	4	21	theme	other	893:897	arg1	agents					899:904	other agents	893:904	other agents	893:904	The systematic optimisation of the conditions improved both the yield of the complex and the IE of the AM agents compared to previously reported methods that have been applied to other agents.
29198147	3	22	theme	mass	483:486	arg1	spectrometry					488:499	Electrospray ionisation mass spectrometry	459:499	Electrospray ionisation mass spectrometry	459:499	Electrospray ionisation mass spectrometry was used to confirm the formation of the thymol/β-CD complex and gas chromatography was used to quantify the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent.
29198147	5	23	theme	optimised	959:967	arg1	parameters					969:978	the optimised parameters	955:978	the optimised parameters	955:978	Using a 1:1 mole ratio of the AM agent to β-CD, the optimised parameters resulted in maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent.
29198147	3	24	used	used	589:592	arg2	chromatography					570:583	gas chromatography	566:583	gas chromatography	566:583	Electrospray ionisation mass spectrometry was used to confirm the formation of the thymol/β-CD complex and gas chromatography was used to quantify the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent.
29198147	4	25	theme	complex	791:797	arg1	IE					807:808	the IE	803:808	the IE of the AM agents	803:825	The systematic optimisation of the conditions improved both the yield of the complex and the IE of the AM agents compared to previously reported methods that have been applied to other agents.
29198147	4	25	theme	complex	791:797	arg1	yield					778:782	the yield	774:782	the yield of the complex	774:797	The systematic optimisation of the conditions improved both the yield of the complex and the IE of the AM agents compared to previously reported methods that have been applied to other agents.
29198147	2	26	theme	inclusion	422:430	arg1	IE					444:445	IE	444:445	IE	444:445	Conditions including solvent composition, temperature, reaction time and total solvent volume were investigated to optimise the inclusion efficiency (IE) and yield.
29198147	2	26	theme	inclusion	422:430	arg1	efficiency					432:441	the inclusion efficiency	418:441	the inclusion efficiency (IE)	418:446	Conditions including solvent composition, temperature, reaction time and total solvent volume were investigated to optimise the inclusion efficiency (IE) and yield.
29198147	0	27	theme	complexes	41:49	arg1	Optimisation					0:11	Optimisation	0:11	Optimisation of β-cyclodextrin inclusion complexes with natural antimicrobial agents: thymol, carvacrol and linalool.	0:116	Optimisation of β-cyclodextrin inclusion complexes with natural antimicrobial agents: thymol, carvacrol and linalool.
29198147	5	28	theme	86	1021:1022	arg1	w/w					1026:1028	w/w	1026:1028	w/w	1026:1028	Using a 1:1 mole ratio of the AM agent to β-CD, the optimised parameters resulted in maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent.
29198147	5	28	theme	86	1021:1022	arg1	%					1023:1023	87, 84 and 86%	1010:1023	%	1023:1023	Using a 1:1 mole ratio of the AM agent to β-CD, the optimised parameters resulted in maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent.
29198147	3	29	theme	amount	610:615	arg1	agent					620:624	the amount AM agent	606:624	the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent	606:711	Electrospray ionisation mass spectrometry was used to confirm the formation of the thymol/β-CD complex and gas chromatography was used to quantify the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent.
29198147	3	30	theme	Electrospray	459:470	arg1	spectrometry					488:499	Electrospray ionisation mass spectrometry	459:499	Electrospray ionisation mass spectrometry	459:499	Electrospray ionisation mass spectrometry was used to confirm the formation of the thymol/β-CD complex and gas chromatography was used to quantify the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent.
29198147	0	31	theme	antimicrobial	64:76	arg1	agents					78:83	natural antimicrobial agents	56:83	natural antimicrobial agents	56:83	Optimisation of β-cyclodextrin inclusion complexes with natural antimicrobial agents: thymol, carvacrol and linalool.
29198147	5	32	theme	%	1023:1023	arg1	yields					1000:1005	maximum yields	992:1005	maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent	992:1124	Using a 1:1 mole ratio of the AM agent to β-CD, the optimised parameters resulted in maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent.
29198147	1	33	theme	Beta-cyclodextrin	118:134	arg1	complexes					153:161	Beta-cyclodextrin (β-CD) inclusion complexes	118:161	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool	118:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	0	34	theme	natural	56:62	arg1	agents					78:83	natural antimicrobial agents	56:83	natural antimicrobial agents	56:83	Optimisation of β-cyclodextrin inclusion complexes with natural antimicrobial agents: thymol, carvacrol and linalool.
29198147	3	35	used	used	505:508	arg2	spectrometry					488:499	Electrospray ionisation mass spectrometry	459:499	Electrospray ionisation mass spectrometry	459:499	Electrospray ionisation mass spectrometry was used to confirm the formation of the thymol/β-CD complex and gas chromatography was used to quantify the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent.
29198147	2	36	theme	reaction	349:356	arg1	time					358:361	reaction time	349:361	reaction time	349:361	Conditions including solvent composition, temperature, reaction time and total solvent volume were investigated to optimise the inclusion efficiency (IE) and yield.
29198147	1	37	dep	agents	205:210	arg1	agents					205:210	naturally derived antimicrobial (AM) agents	168:210	naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool	168:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	37	dep	agents	205:210	arg1	linalool					235:242	linalool	235:242	linalool	235:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	37	dep	agents	205:210	arg1	carvacrol					221:229	carvacrol	221:229	carvacrol	221:229	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	1	37	dep	agents	205:210	arg1	thymol					213:218	thymol	213:218	thymol	213:218	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	0	38	with	Optimisation	0:11	arg1	agents					78:83	natural antimicrobial agents	56:83	natural antimicrobial agents	56:83	Optimisation of β-cyclodextrin inclusion complexes with natural antimicrobial agents: thymol, carvacrol and linalool.
29198147	4	39	theme	systematic	718:727	arg1	optimisation					729:740	The systematic optimisation	714:740	The systematic optimisation of the conditions	714:758	The systematic optimisation of the conditions improved both the yield of the complex and the IE of the AM agents compared to previously reported methods that have been applied to other agents.
29198147	3	40	theme	gas	566:568	arg1	chromatography					570:583	gas chromatography	566:583	gas chromatography	566:583	Electrospray ionisation mass spectrometry was used to confirm the formation of the thymol/β-CD complex and gas chromatography was used to quantify the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent.
29198147	5	41	theme	87	1010:1011	arg1	w/w					1026:1028	w/w	1026:1028	w/w	1026:1028	Using a 1:1 mole ratio of the AM agent to β-CD, the optimised parameters resulted in maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent.
29198147	5	41	theme	87	1010:1011	arg1	%					1023:1023	87, 84 and 86%	1010:1023	%	1023:1023	Using a 1:1 mole ratio of the AM agent to β-CD, the optimised parameters resulted in maximum yields of 87, 84 and 86% (w/w) for thymol, carvacrol and linalool, respectively, with IE's close to 100% (w/w) for each agent.
29198147	3	42	theme	AM	617:618	arg1	agent					620:624	the amount AM agent	606:624	the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent	606:711	Electrospray ionisation mass spectrometry was used to confirm the formation of the thymol/β-CD complex and gas chromatography was used to quantify the amount AM agent that was encapsulated, absorbed onto the surface, or remaining in the filtered solvent.
29198147	1	43	theme	inclusion	143:151	arg1	complexes					153:161	Beta-cyclodextrin (β-CD) inclusion complexes	118:161	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool	118:242	Beta-cyclodextrin (β-CD) inclusion complexes with naturally derived antimicrobial (AM) agents: thymol, carvacrol and linalool were prepared using a co-precipitation technique.
29198147	0	44	dep	Optimisation	0:11	arg1	thymol					86:91	thymol	86:91	thymol	86:91	Optimisation of β-cyclodextrin inclusion complexes with natural antimicrobial agents: thymol, carvacrol and linalool.
29198147	0	44	dep	Optimisation	0:11	arg1	linalool					108:115	linalool	108:115	linalool	108:115	Optimisation of β-cyclodextrin inclusion complexes with natural antimicrobial agents: thymol, carvacrol and linalool.
29198147	0	44	dep	Optimisation	0:11	arg1	carvacrol					94:102	carvacrol	94:102	carvacrol	94:102	Optimisation of β-cyclodextrin inclusion complexes with natural antimicrobial agents: thymol, carvacrol and linalool.
29198147	2	45	theme	solvent	373:379	arg1	volume					381:386	total solvent volume	367:386	total solvent volume	367:386	Conditions including solvent composition, temperature, reaction time and total solvent volume were investigated to optimise the inclusion efficiency (IE) and yield.
24274537	5	0	theme	coated	700:705	arg1	paper					707:711	coated paper	700:711	coated paper	700:711	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	5	1	dep	significantly	635:647	arg1	p<0.05					650:655	p<0.05	650:655	p<0.05	650:655	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	3	2	theme	papers	473:478	arg1	properties					452:461	the physical and mechanical properties	424:461	the physical and mechanical properties of coated papers	424:478	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	3	3	dep	concentration	323:335	arg1	%					353:353	12%	351:353	12%	351:353	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	3	3	dep	concentration	323:335	arg1	%					339:339	7%	338:339	7%	338:339	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	3	3	dep	concentration	323:335	arg1	%					344:344	10%	342:344	10%	342:344	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	3	3	dep	concentration	323:335	arg1	w/w					356:358	w/w	356:358	w/w	356:358	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	6	4	theme	second	756:761	arg1	layer					763:767	a second layer	754:767	a second layer on wet or dry caseinate films	754:797	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	6	5	theme	wet	772:774	arg1	films					793:797	wet or dry caseinate films	772:797	wet or dry caseinate films	772:797	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	7	6	theme	caseinate-coated	983:998	arg1	paper					1000:1004	the dried preformed caseinate-coated paper	963:1004	the dried preformed caseinate-coated paper	963:1004	The greatest reduction in paper WVP is achieved by addition of a chitosan layer to the dried preformed caseinate-coated paper.
24274537	6	7	theme	chitosan	742:749	arg1	application					727:737	the application	723:737	the application of chitosan as a second layer on wet or dry caseinate films	723:797	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	4	8	theme	vapor	563:567	arg1	WVP					583:585	WVP	583:585	WVP	583:585	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	4	8	theme	vapor	563:567	arg1	permeability					569:580	water vapor permeability	557:580	water vapor permeability (WVP) of the resulting coated paper sheets	557:623	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	2	9	theme	caseinate/chitosan	173:190	arg1	films					192:196	Caseinate, chitosan and caseinate/chitosan films	149:196	Caseinate, chitosan and caseinate/chitosan films	149:196	Caseinate, chitosan and caseinate/chitosan films were preliminary characterized by FTIR spectroscopy and thermal stability analyses.
24274537	6	10	theme	paper	873:877	arg1	strength					849:856	the tensile strength	837:856	(p>0.05) the tensile strength (TS) of coated paper	828:877	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	6	10	theme	paper	873:877	arg1	p>0.05					829:834	p>0.05	829:834	p>0.05	829:834	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	6	10	theme	paper	873:877	arg1	TS					859:860	TS	859:860	TS	859:860	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	4	11	theme	water	557:561	arg1	WVP					583:585	WVP	583:585	WVP	583:585	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	4	11	theme	water	557:561	arg1	permeability					569:580	water vapor permeability	557:580	water vapor permeability (WVP) of the resulting coated paper sheets	557:623	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	5	12	theme	%	693:693	arg1	break					686:690	the elongation at break	668:690	the elongation at break (%E) of coated paper	668:711	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	5	12	theme	%	693:693	arg1	E					694:694	%E	693:694	%E	693:694	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	7	13	theme	dried	967:971	arg1	paper					1000:1004	the dried preformed caseinate-coated paper	963:1004	the dried preformed caseinate-coated paper	963:1004	The greatest reduction in paper WVP is achieved by addition of a chitosan layer to the dried preformed caseinate-coated paper.
24274537	4	14	theme	sheets	618:623	arg1	WVP					583:585	WVP	583:585	WVP	583:585	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	4	14	theme	sheets	618:623	arg1	permeability					569:580	water vapor permeability	557:580	water vapor permeability (WVP) of the resulting coated paper sheets	557:623	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	4	15	from	decrease	545:552	arg1	WVP					583:585	WVP	583:585	WVP	583:585	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	4	15	from	decrease	545:552	arg1	permeability					569:580	water vapor permeability	557:580	water vapor permeability (WVP) of the resulting coated paper sheets	557:623	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	2	16	theme	chitosan	160:167	arg1	films					192:196	Caseinate, chitosan and caseinate/chitosan films	149:196	Caseinate, chitosan and caseinate/chitosan films	149:196	Caseinate, chitosan and caseinate/chitosan films were preliminary characterized by FTIR spectroscopy and thermal stability analyses.
24274537	3	17	theme	coating	297:303	arg1	weight					305:310	coating weight	297:310	coating weight	297:310	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	7	18	theme	layer	954:958	arg1	addition					931:938	addition	931:938	addition of a chitosan layer to the dried preformed caseinate-coated paper	931:1004	The greatest reduction in paper WVP is achieved by addition of a chitosan layer to the dried preformed caseinate-coated paper.
24274537	0	19	theme	Chitosan-caseinate	0:17	arg1	coatings					27:34	Chitosan-caseinate bilayer coatings	0:34	Chitosan-caseinate bilayer coatings for paper packaging materials	0:64	Chitosan-caseinate bilayer coatings for paper packaging materials.
24274537	4	20	theme	paper	612:616	arg1	sheets					618:623	the resulting coated paper sheets	591:623	the resulting coated paper sheets	591:623	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	0	21	theme	bilayer	19:25	arg1	coatings					27:34	Chitosan-caseinate bilayer coatings	0:34	Chitosan-caseinate bilayer coatings for paper packaging materials	0:64	Chitosan-caseinate bilayer coatings for paper packaging materials.
24274537	5	22	theme	paper	707:711	arg1	break					686:690	the elongation at break	668:690	the elongation at break (%E) of coated paper	668:711	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	5	22	theme	paper	707:711	arg1	E					694:694	%E	693:694	%E	693:694	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	5	23	theme	elongation	672:681	arg1	break					686:690	the elongation at break	668:690	the elongation at break (%E) of coated paper	668:711	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	5	23	theme	elongation	672:681	arg1	E					694:694	%E	693:694	%E	693:694	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	3	24	theme	coating	366:372	arg1	application					374:384	coating application	366:384	coating application	366:384	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	6	25	theme	coated	866:871	arg1	paper					873:877	coated paper	866:877	coated paper	866:877	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	1	26	theme	caseinate/chitosan	100:117	arg1	films					127:131	caseinate/chitosan bilayer films	100:131	caseinate/chitosan bilayer films	100:131	Papers coated with caseinate and caseinate/chitosan bilayer films were developed.
24274537	7	27	from	reduction	893:901	arg1	paper					906:910	paper WVP	906:914	paper WVP	906:914	The greatest reduction in paper WVP is achieved by addition of a chitosan layer to the dried preformed caseinate-coated paper.
24274537	3	28	from	effects	286:292	arg1	properties					452:461	the physical and mechanical properties	424:461	the physical and mechanical properties of coated papers	424:478	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	3	29	theme	caseinate	313:321	arg1	concentration					323:335	caseinate concentration	313:335	caseinate concentration (7%, 10%, and 12%, w/w)	313:359	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	1	30	theme	bilayer	119:125	arg1	films					127:131	caseinate/chitosan bilayer films	100:131	caseinate/chitosan bilayer films	100:131	Papers coated with caseinate and caseinate/chitosan bilayer films were developed.
24274537	7	31	theme	preformed	973:981	arg1	paper					1000:1004	the dried preformed caseinate-coated paper	963:1004	the dried preformed caseinate-coated paper	963:1004	The greatest reduction in paper WVP is achieved by addition of a chitosan layer to the dried preformed caseinate-coated paper.
24274537	3	32	theme	physical	428:435	arg1	properties					452:461	the physical and mechanical properties	424:461	the physical and mechanical properties of coated papers	424:478	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	3	33	theme	concentration	323:335	arg1	effects					286:292	The effects	282:292	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers	282:478	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	6	34	theme	caseinate	783:791	arg1	films					793:797	wet or dry caseinate films	772:797	wet or dry caseinate films	772:797	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	0	35	theme	packaging	46:54	arg1	materials					56:64	paper packaging materials	40:64	paper packaging materials	40:64	Chitosan-caseinate bilayer coatings for paper packaging materials.
24274537	3	36	theme	weight	305:310	arg1	effects					286:292	The effects	282:292	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers	282:478	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	6	37	theme	dry	779:781	arg1	films					793:797	wet or dry caseinate films	772:797	wet or dry caseinate films	772:797	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	4	38	theme	coated	605:610	arg1	sheets					618:623	the resulting coated paper sheets	591:623	the resulting coated paper sheets	591:623	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	0	39	theme	paper	40:44	arg1	materials					56:64	paper packaging materials	40:64	paper packaging materials	40:64	Chitosan-caseinate bilayer coatings for paper packaging materials.
24274537	3	40	theme	single	395:400	arg1	layer					402:406	single layer	395:406	single layer	395:406	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	5	41	from	break	686:690	arg1	elongation					672:681	the elongation at break	668:690	the elongation at break (%E) of coated paper	668:711	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	5	41	from	break	686:690	arg1	at					683:684	the elongation at break	668:690	the elongation at break (%E) of coated paper	668:711	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	6	42	from	layer	763:767	arg1	films					793:797	wet or dry caseinate films	772:797	wet or dry caseinate films	772:797	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	4	43	theme	resulting	595:603	arg1	sheets					618:623	the resulting coated paper sheets	591:623	the resulting coated paper sheets	591:623	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	3	44	theme	application	374:384	arg1	effects					286:292	The effects	282:292	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers	282:478	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	4	45	theme	caseinate	526:534	arg1	concentration					509:521	the concentration	505:521	the concentration of caseinate	505:534	Increasing the concentration of caseinate led to a decrease in water vapor permeability (WVP) of the resulting coated paper sheets.
24274537	5	46	theme	at	683:684	arg1	break					686:690	the elongation at break	668:690	the elongation at break (%E) of coated paper	668:711	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	5	46	theme	at	683:684	arg1	E					694:694	%E	693:694	%E	693:694	Chitosan significantly (p<0.05) increased the elongation at break (%E) of coated paper.
24274537	6	47	theme	tensile	841:847	arg1	strength					849:856	the tensile strength	837:856	(p>0.05) the tensile strength (TS) of coated paper	828:877	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	6	47	theme	tensile	841:847	arg1	p>0.05					829:834	p>0.05	829:834	p>0.05	829:834	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	6	47	theme	tensile	841:847	arg1	TS					859:860	TS	859:860	TS	859:860	However, the application of chitosan as a second layer on wet or dry caseinate films did not significantly affect (p>0.05) the tensile strength (TS) of coated paper.
24274537	7	48	theme	chitosan	945:952	arg1	layer					954:958	a chitosan layer	943:958	a chitosan layer	943:958	The greatest reduction in paper WVP is achieved by addition of a chitosan layer to the dried preformed caseinate-coated paper.
24274537	2	49	theme	thermal	254:260	arg1	stability					262:270	thermal stability	254:270	thermal stability	254:270	Caseinate, chitosan and caseinate/chitosan films were preliminary characterized by FTIR spectroscopy and thermal stability analyses.
24274537	3	50	theme	coated	466:471	arg1	papers					473:478	coated papers	466:478	coated papers	466:478	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	3	51	theme	mechanical	441:450	arg1	properties					452:461	the physical and mechanical properties	424:461	the physical and mechanical properties of coated papers	424:478	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	2	52	theme	FTIR	232:235	arg1	spectroscopy					237:248	FTIR spectroscopy	232:248	FTIR spectroscopy	232:248	Caseinate, chitosan and caseinate/chitosan films were preliminary characterized by FTIR spectroscopy and thermal stability analyses.
24274537	3	53	dep	weight	305:310	arg1	bilayer					412:418	bilayer	412:418	bilayer	412:418	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	3	53	dep	weight	305:310	arg1	methods					386:392	methods	386:392	methods	386:392	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	3	53	dep	weight	305:310	arg1	layer					402:406	single layer	395:406	single layer	395:406	The effects of coating weight, caseinate concentration (7%, 10%, and 12%, w/w), and coating application methods (single layer and bilayer) on the physical and mechanical properties of coated papers were studied.
24274537	7	54	theme	greatest	884:891	arg1	reduction					893:901	The greatest reduction	880:901	The greatest reduction in paper WVP	880:914	The greatest reduction in paper WVP is achieved by addition of a chitosan layer to the dried preformed caseinate-coated paper.
24274537	2	55	theme	Caseinate	149:157	arg1	films					192:196	Caseinate, chitosan and caseinate/chitosan films	149:196	Caseinate, chitosan and caseinate/chitosan films	149:196	Caseinate, chitosan and caseinate/chitosan films were preliminary characterized by FTIR spectroscopy and thermal stability analyses.
24274537	2	56	dep	spectroscopy	237:248	arg1	analyses					272:279	analyses	272:279	analyses	272:279	Caseinate, chitosan and caseinate/chitosan films were preliminary characterized by FTIR spectroscopy and thermal stability analyses.
26283165	2	0	theme	repair	338:343	arg1	applications					345:356	tissue repair applications	331:356	tissue repair applications	331:356	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	10	1	theme	micromachined	1776:1788	arg1	composites					1808:1817	Laser micromachined collagen-alginate composites	1770:1817	Laser micromachined collagen-alginate composites containing hMSCs	1770:1834	Laser micromachined collagen-alginate composites containing hMSCs can be used to bridge soft tissue defects with the capacity for enhanced tissue repair and integration.
26283165	11	2	theme	restoration	1976:1986	arg1	STATEMENT					1940:1948	STATEMENT	1940:1948	STATEMENT OF SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications	1940:2059	STATEMENT OF SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications represents a critical challenge in the clinic.
26283165	11	3	theme	Effective	1966:1974	arg1	restoration					1976:1986	SIGNIFICANCE Effective restoration	1953:1986	SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications	1953:2059	STATEMENT OF SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications represents a critical challenge in the clinic.
26283165	6	4	theme	cell	929:932	arg1	viability					934:942	in vitro, high cell viability	914:942	in vitro, high cell viability	914:942	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	15	5	theme	matrix	2758:2763	arg1	production					2765:2774	matrix production	2758:2774	matrix production	2758:2774	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	2	6	theme	collagen-alginate	251:267	arg1	construct					269:277	a composite, laser micromachined, collagen-alginate construct	217:277	construct	269:277	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	15	7	theme	defect	2595:2600	arg1	repair					2553:2558	repair	2553:2558	repair of a full thickness abdominal wall defect in a rat model	2553:2615	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	8	8	dep	N/mm	1405:1408	arg1	N/mm					1437:1440	0.59 ± 0.25 N/mm	1425:1440	0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm	1393:1440	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	13	9	used	used	2247:2250	arg2	micromachining					2228:2241	Laser micromachining	2222:2241	Laser micromachining	2222:2241	Laser micromachining was used to fabricate patterned, microporous constructs designed with pores of defined size and distribution as a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration.
26283165	11	10	theme	tissue	2002:2007	arg1	defects					2009:2015	large soft tissue defects	1991:2015	large soft tissue defects caused by trauma or treatment complications	1991:2059	STATEMENT OF SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications represents a critical challenge in the clinic.
26283165	15	11	theme	abdominal	2580:2588	arg1	defect					2595:2600	a full thickness abdominal wall defect	2563:2600	a full thickness abdominal wall defect	2563:2600	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	1	12	theme	host	186:189	arg1	tissue					191:196	the host tissue	182:196	the host tissue	182:196	A critical challenge in tissue regeneration is to develop constructs that effectively integrate with the host tissue.
26283165	6	13	theme	MCP-1	998:1002	arg1	expression					1012:1021	MCP-1 protein expression	998:1021	MCP-1 protein expression	998:1021	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	4	14	theme	laser	647:651	arg1	constructs					677:686	laser micromachined patterned constructs	647:686	laser micromachined patterned constructs	647:686	Significantly, laser micromachined patterned constructs displayed both substantially greater compliance and suture retention strength than non-patterned constructs.
26283165	10	15	theme	enhanced	1900:1907	arg1	repair					1916:1921	enhanced tissue repair	1900:1921	enhanced tissue repair	1900:1921	Laser micromachined collagen-alginate composites containing hMSCs can be used to bridge soft tissue defects with the capacity for enhanced tissue repair and integration.
26283165	7	16	theme	hernia	1107:1112	arg1	recurrence					1114:1123	hernia recurrence	1107:1123	hernia recurrence	1107:1123	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	8	17	dep	cell	1584:1587	arg1	p<0.05					1653:1658	p<0.05	1653:1658	p<0.05	1653:1658	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	8	17	dep	cell	1584:1587	arg1	μm					1607:1608	2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf	1597:1650	2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf	1597:1650	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	2	18	theme	mesenchymal	296:306	arg1	hMSCs					320:324	hMSCs	320:324	hMSCs	320:324	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	2	18	theme	mesenchymal	296:306	arg1	cells					313:317	human mesenchymal stem cells	290:317	human mesenchymal stem cells (hMSCs)	290:325	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	15	19	theme	full	2565:2568	arg1	defect					2595:2600	a full thickness abdominal wall defect	2563:2600	a full thickness abdominal wall defect	2563:2600	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	13	20	theme	patterned	2265:2273	arg1	constructs					2288:2297	patterned, microporous constructs	2265:2297	patterned, microporous constructs designed with pores of defined size and distribution	2265:2350	Laser micromachining was used to fabricate patterned, microporous constructs designed with pores of defined size and distribution as a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration.
26283165	13	20	theme	patterned	2265:2273	arg1	means					2357:2361	a means	2355:2361	a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration	2355:2466	Laser micromachining was used to fabricate patterned, microporous constructs designed with pores of defined size and distribution as a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration.
26283165	6	21	theme	PDGF-β	987:992	arg1	levels					971:976	constant levels	962:976	constant levels of VEGF, PDGF-β and MCP-1 protein expression	962:1021	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	11	22	theme	defects	2009:2015	arg1	restoration					1976:1986	SIGNIFICANCE Effective restoration	1953:1986	SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications	1953:2059	STATEMENT OF SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications represents a critical challenge in the clinic.
26283165	4	23	theme	patterned	667:675	arg1	constructs					677:686	laser micromachined patterned constructs	647:686	laser micromachined patterned constructs	647:686	Significantly, laser micromachined patterned constructs displayed both substantially greater compliance and suture retention strength than non-patterned constructs.
26283165	7	24	theme	abdominal	1044:1052	arg1	model					1066:1070	a full thickness abdominal wall defect model	1027:1070	a full thickness abdominal wall defect model	1027:1070	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	8	25	dep	cell	1380:1383	arg1	acellular					1414:1422	acellular	1414:1422	acellular	1414:1422	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	8	25	dep	cell	1380:1383	arg1	N/mm					1405:1408	0.92 ± 0.19 N/mm	1393:1408	0.92 ± 0.19 N/mm	1393:1408	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	8	25	dep	cell	1380:1383	arg1	p=0.01					1443:1448	p=0.01	1443:1448	p=0.01	1443:1448	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	6	26	theme	VEGF	981:984	arg1	levels					971:976	constant levels	962:976	constant levels of VEGF, PDGF-β and MCP-1 protein expression	962:1021	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	15	27	theme	tissue	2807:2812	arg1	integration					2814:2824	tissue integration	2807:2824	tissue integration	2807:2824	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	7	28	theme	full	1029:1032	arg1	model					1066:1070	a full thickness abdominal wall defect model	1027:1070	a full thickness abdominal wall defect model	1027:1070	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	15	29	theme	innate	2657:2662	arg1	immunity					2664:2671	host innate immunity	2652:2671	host innate immunity	2652:2671	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	4	30	theme	non-patterned	771:783	arg1	constructs					785:794	non-patterned constructs	771:794	non-patterned constructs	771:794	Significantly, laser micromachined patterned constructs displayed both substantially greater compliance and suture retention strength than non-patterned constructs.
26283165	2	31	theme	micromachined	236:248	arg1	construct					269:277	a composite, laser micromachined, collagen-alginate construct	217:277	construct	269:277	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	8	32	theme	integration	1358:1368	arg1	strength					1370:1377	greater integration strength	1350:1377	greater integration strength	1350:1377	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	7	33	theme	abdominal	1256:1264	arg1	viscera					1266:1272	underlying abdominal viscera	1245:1272	underlying abdominal viscera	1245:1272	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	3	34	theme	distribution	544:555	arg1	pores					505:509	pores	505:509	pores of defined dimension and spatial distribution	505:555	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	8	35	dep	cell	1479:1482	arg1	acellular					1508:1516	acellular	1508:1516	acellular	1508:1516	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	8	35	dep	cell	1479:1482	arg1	2.7-2.1/hpf					1492:1502	2.7-2.1/hpf	1492:1502	2.7-2.1/hpf	1492:1502	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	8	35	dep	cell	1479:1482	arg1	p<0.03					1532:1537	p<0.03	1532:1537	p<0.03	1532:1537	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	8	35	dep	cell	1479:1482	arg1	1.7-2.1/hpf					1519:1529	1.7-2.1/hpf	1519:1529	1.7-2.1/hpf	1519:1529	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	11	36	theme	treatment	2037:2045	arg1	complications					2047:2059	treatment complications	2037:2059	treatment complications	2037:2059	STATEMENT OF SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications represents a critical challenge in the clinic.
26283165	4	37	theme	suture	740:745	arg1	strength					757:764	suture retention strength	740:764	suture retention strength	740:764	Significantly, laser micromachined patterned constructs displayed both substantially greater compliance and suture retention strength than non-patterned constructs.
26283165	5	38	theme	RGD-functionalized	821:838	arg1	gel					849:851	an RGD-functionalized alginate gel	818:851	an RGD-functionalized alginate gel modified to degrade in vivo	818:879	hMSCs were loaded in an RGD-functionalized alginate gel modified to degrade in vivo.
26283165	13	39	theme	Laser	2222:2226	arg1	micromachining					2228:2241	Laser micromachining	2222:2241	Laser micromachining	2222:2241	Laser micromachining was used to fabricate patterned, microporous constructs designed with pores of defined size and distribution as a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration.
26283165	9	40	theme	macrophages	1692:1702	arg1	ratio					1680:1684	the ratio	1676:1684	the ratio of M1 macrophages to total macrophages	1676:1723	A decrease in the ratio of M1 macrophages to total macrophages was also observed in hMSC-populated samples.
26283165	12	41	theme	cell	2189:2192	arg1	delivery					2194:2201	stem cell delivery	2184:2201	stem cell delivery	2184:2201	In this study, a novel composite construct was engineered and evaluated for stem cell delivery and tissue repair.
26283165	15	42	theme	composite	2622:2630	arg1	construct					2632:2640	the composite construct	2618:2640	the composite construct	2618:2640	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	8	43	dep	μm	1607:1608	arg1	2					1610:1610	2	1610:1610	2	1610:1610	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	8	43	dep	μm	1607:1608	arg1	/hpf					1647:1650	1570-2530 μm(2)/hpf	1632:1650	2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf	1597:1650	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	8	43	dep	μm	1607:1608	arg1	acellular					1621:1629	acellular	1621:1629	acellular	1621:1629	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	8	43	dep	μm	1607:1608	arg1	/hpf					1612:1615	/hpf	1612:1615	/hpf	1612:1615	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	6	44	theme	expression	1012:1021	arg1	levels					971:976	constant levels	962:976	constant levels of VEGF, PDGF-β and MCP-1 protein expression	962:1021	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	9	45	theme	total	1707:1711	arg1	macrophages					1713:1723	total macrophages	1707:1723	total macrophages	1707:1723	A decrease in the ratio of M1 macrophages to total macrophages was also observed in hMSC-populated samples.
26283165	0	46	from	therapy	42:48	arg1	applications					67:78	tissue repair applications	53:78	tissue repair applications	53:78	Engineered composite fascia for stem cell therapy in tissue repair applications.
26283165	8	47	theme	0.92	1393:1396	arg1	±					1398:1398	±	1398:1398	±	1398:1398	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	0	48	theme	cell	37:40	arg1	therapy					42:48	stem cell therapy	32:48	stem cell therapy in tissue repair applications	32:78	Engineered composite fascia for stem cell therapy in tissue repair applications.
26283165	0	49	theme	repair	60:65	arg1	applications					67:78	tissue repair applications	53:78	tissue repair applications	53:78	Engineered composite fascia for stem cell therapy in tissue repair applications.
26283165	8	50	theme	0.19	1400:1403	arg1	±					1398:1398	±	1398:1398	±	1398:1398	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	1	51	theme	critical	83:90	arg1	challenge					92:100	A critical challenge	81:100	A critical challenge in tissue regeneration	81:123	A critical challenge in tissue regeneration is to develop constructs that effectively integrate with the host tissue.
26283165	14	52	theme	engineered	2506:2515	arg1	gel					2526:2528	an engineered alginate gel	2503:2528	an engineered alginate gel containing hMSCs	2503:2545	The construct was embedded within an engineered alginate gel containing hMSCs.
26283165	2	53	theme	laser	230:234	arg1	construct					269:277	a composite, laser micromachined, collagen-alginate construct	217:277	construct	269:277	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	8	54	dep	/hpf	1647:1650	arg1	2					1645:1645	2	1645:1645	2	1645:1645	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	7	55	theme	vascular	1186:1193	arg1	formation					1203:1211	de novo tissue and vascular network formation	1167:1211	formation	1203:1211	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	2	56	contain	containing	279:288	arg2	hMSCs					320:324	hMSCs	320:324	hMSCs	320:324	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	2	56	contain	containing	279:288	arg2	cells					313:317	human mesenchymal stem cells	290:317	human mesenchymal stem cells (hMSCs)	290:325	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	2	56	contain	containing	279:288	arg1	construct					269:277	a composite, laser micromachined, collagen-alginate construct	217:277	construct	269:277	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	14	57	theme	alginate	2517:2524	arg1	gel					2526:2528	an engineered alginate gel	2503:2528	an engineered alginate gel containing hMSCs	2503:2545	The construct was embedded within an engineered alginate gel containing hMSCs.
26283165	3	58	theme	collagen	404:411	arg1	sheets					413:418	laminated collagen sheets	394:418	laminated collagen sheets	394:418	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	12	59	theme	composite	2131:2139	arg1	construct					2141:2149	a novel composite construct	2123:2149	a novel composite construct	2123:2149	In this study, a novel composite construct was engineered and evaluated for stem cell delivery and tissue repair.
26283165	3	60	theme	robust	443:448	arg1	fascia					450:455	a mechanically robust fascia	428:455	a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration	428:629	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	3	60	theme	robust	443:448	arg1	means					562:566	a means	560:566	a means to modulate mechanical behavior and promote tissue integration	560:629	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	0	61	theme	stem	32:35	arg1	therapy					42:48	stem cell therapy	32:48	stem cell therapy in tissue repair applications	32:78	Engineered composite fascia for stem cell therapy in tissue repair applications.
26283165	15	62	theme	reparative	2683:2692	arg1	response					2705:2712	a reparative phenotypic response	2681:2712	a reparative phenotypic response	2681:2712	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	0	63	theme	tissue	53:58	arg1	applications					67:78	tissue repair applications	53:78	tissue repair applications	53:78	Engineered composite fascia for stem cell therapy in tissue repair applications.
26283165	2	64	theme	tissue	331:336	arg1	applications					345:356	tissue repair applications	331:356	tissue repair applications	331:356	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	7	65	theme	de	1167:1168	arg1	tissue					1175:1180	de novo tissue and vascular network formation	1167:1211	tissue	1175:1180	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	6	66	theme	day	891:893	arg1	period					907:912	a 7 day observation period	887:912	a 7 day observation period	887:912	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	9	67	located	observed	1734:1741	arg1	samples					1761:1767	hMSC-populated samples	1746:1767	hMSC-populated samples	1746:1767	A decrease in the ratio of M1 macrophages to total macrophages was also observed in hMSC-populated samples.
26283165	9	67	located	observed	1734:1741	arg2	decrease					1664:1671	A decrease	1662:1671	A decrease in the ratio of M1 macrophages to total macrophages	1662:1723	A decrease in the ratio of M1 macrophages to total macrophages was also observed in hMSC-populated samples.
26283165	6	68	dep	in	914:915	arg1	high					924:927	high	924:927	high	924:927	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	6	68	dep	in	914:915	arg1	vitro					917:921	vitro	917:921	vitro	917:921	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	3	69	theme	spatial	536:542	arg1	distribution					544:555	spatial distribution	536:555	spatial distribution	536:555	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	13	70	theme	defined	2322:2328	arg1	size					2330:2333	size	2330:2333	size	2330:2333	Laser micromachining was used to fabricate patterned, microporous constructs designed with pores of defined size and distribution as a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration.
26283165	4	71	theme	greater	717:723	arg1	compliance					725:734	substantially greater compliance	703:734	substantially greater compliance	703:734	Significantly, laser micromachined patterned constructs displayed both substantially greater compliance and suture retention strength than non-patterned constructs.
26283165	3	72	theme	defined	514:520	arg1	dimension					522:530	defined dimension	514:530	defined dimension	514:530	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	6	73	theme	constant	962:969	arg1	levels					971:976	constant levels	962:976	constant levels of VEGF, PDGF-β and MCP-1 protein expression	962:1021	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	10	74	theme	soft	1858:1861	arg1	defects					1870:1876	soft tissue defects	1858:1876	soft tissue defects	1858:1876	Laser micromachined collagen-alginate composites containing hMSCs can be used to bridge soft tissue defects with the capacity for enhanced tissue repair and integration.
26283165	7	75	theme	8-week	1148:1153	arg1	period					1155:1160	an 8-week period	1145:1160	an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera	1145:1272	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	8	76	theme	μm	1642:1643	arg1	/hpf					1647:1650	1570-2530 μm(2)/hpf	1632:1650	2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf	1597:1650	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	7	77	with	period	1155:1160	arg1	absence					1221:1227	the absence	1217:1227	the absence of adhesions to underlying abdominal viscera	1217:1272	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	7	77	with	period	1155:1160	arg1	tissue					1175:1180	de novo tissue and vascular network formation	1167:1211	tissue	1175:1180	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	7	77	with	period	1155:1160	arg1	formation					1203:1211	de novo tissue and vascular network formation	1167:1211	formation	1203:1211	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	2	78	theme	human	290:294	arg1	hMSCs					320:324	hMSCs	320:324	hMSCs	320:324	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	2	78	theme	human	290:294	arg1	cells					313:317	human mesenchymal stem cells	290:317	human mesenchymal stem cells (hMSCs)	290:325	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	11	79	theme	critical	2074:2081	arg1	challenge					2083:2091	a critical challenge	2072:2091	a critical challenge	2072:2091	STATEMENT OF SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications represents a critical challenge in the clinic.
26283165	7	80	theme	Wistar	1128:1133	arg1	rats					1135:1138	Wistar rats	1128:1138	Wistar rats	1128:1138	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	15	81	theme	rat	2607:2609	arg1	model					2611:2615	a rat model	2605:2615	a rat model	2605:2615	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	11	82	theme	soft	1997:2000	arg1	defects					2009:2015	large soft tissue defects	1991:2015	large soft tissue defects caused by trauma or treatment complications	1991:2059	STATEMENT OF SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications represents a critical challenge in the clinic.
26283165	6	83	theme	protein	1004:1010	arg1	expression					1012:1021	MCP-1 protein expression	998:1021	MCP-1 protein expression	998:1021	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	10	84	theme	tissue	1909:1914	arg1	repair					1916:1921	enhanced tissue repair	1900:1921	enhanced tissue repair	1900:1921	Laser micromachined collagen-alginate composites containing hMSCs can be used to bridge soft tissue defects with the capacity for enhanced tissue repair and integration.
26283165	2	85	theme	stem	308:311	arg1	hMSCs					320:324	hMSCs	320:324	hMSCs	320:324	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	2	85	theme	stem	308:311	arg1	cells					313:317	human mesenchymal stem cells	290:317	human mesenchymal stem cells (hMSCs)	290:325	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	15	86	theme	wall	2590:2593	arg1	defect					2595:2600	a full thickness abdominal wall defect	2563:2600	a full thickness abdominal wall defect	2563:2600	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	14	87	contain	containing	2530:2539	arg1	gel					2526:2528	an engineered alginate gel	2503:2528	an engineered alginate gel containing hMSCs	2503:2545	The construct was embedded within an engineered alginate gel containing hMSCs.
26283165	14	87	contain	containing	2530:2539	arg2	hMSCs					2541:2545	hMSCs	2541:2545	hMSCs	2541:2545	The construct was embedded within an engineered alginate gel containing hMSCs.
26283165	7	88	theme	composite	1077:1085	arg1	construct					1087:1095	the composite construct	1073:1095	the composite construct	1073:1095	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	2	89	theme	composite	219:227	arg1	construct					269:277	a composite, laser micromachined, collagen-alginate construct	217:277	construct	269:277	Here, we describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications.
26283165	15	90	theme	thickness	2570:2578	arg1	defect					2595:2600	a full thickness abdominal wall defect	2563:2600	a full thickness abdominal wall defect	2563:2600	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	4	91	theme	micromachined	653:665	arg1	constructs					677:686	laser micromachined patterned constructs	647:686	laser micromachined patterned constructs	647:686	Significantly, laser micromachined patterned constructs displayed both substantially greater compliance and suture retention strength than non-patterned constructs.
26283165	13	92	theme	incorporated	2417:2428	arg1	cells					2430:2434	incorporated cells	2417:2434	incorporated cells	2417:2434	Laser micromachining was used to fabricate patterned, microporous constructs designed with pores of defined size and distribution as a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration.
26283165	3	93	theme	mechanical	580:589	arg1	behavior					591:598	mechanical behavior	580:598	mechanical behavior	580:598	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	7	94	theme	wall	1054:1057	arg1	model					1066:1070	a full thickness abdominal wall defect model	1027:1070	a full thickness abdominal wall defect model	1027:1070	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	1	95	theme	tissue	105:110	arg1	regeneration					112:123	tissue regeneration	105:123	tissue regeneration	105:123	A critical challenge in tissue regeneration is to develop constructs that effectively integrate with the host tissue.
26283165	4	96	theme	retention	747:755	arg1	strength					757:764	suture retention strength	740:764	suture retention strength	740:764	Significantly, laser micromachined patterned constructs displayed both substantially greater compliance and suture retention strength than non-patterned constructs.
26283165	7	97	theme	defect	1059:1064	arg1	model					1066:1070	a full thickness abdominal wall defect model	1027:1070	a full thickness abdominal wall defect model	1027:1070	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	8	98	dep	increased	1545:1553	arg1	cell					1584:1587	cell	1584:1587	cell seeded	1584:1594	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	10	99	theme	Laser	1770:1774	arg1	composites					1808:1817	Laser micromachined collagen-alginate composites	1770:1817	Laser micromachined collagen-alginate composites containing hMSCs	1770:1834	Laser micromachined collagen-alginate composites containing hMSCs can be used to bridge soft tissue defects with the capacity for enhanced tissue repair and integration.
26283165	7	100	theme	thickness	1034:1042	arg1	model					1066:1070	a full thickness abdominal wall defect model	1027:1070	a full thickness abdominal wall defect model	1027:1070	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	9	101	from	decrease	1664:1671	arg1	ratio					1680:1684	the ratio	1676:1684	the ratio of M1 macrophages to total macrophages	1676:1723	A decrease in the ratio of M1 macrophages to total macrophages was also observed in hMSC-populated samples.
26283165	8	102	contain	containing	1323:1332	arg1	constructs					1312:1321	constructs	1312:1321	constructs containing hMSCs	1312:1338	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	8	102	contain	containing	1323:1332	arg2	hMSCs					1334:1338	hMSCs	1334:1338	hMSCs	1334:1338	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	7	103	theme	underlying	1245:1254	arg1	viscera					1266:1272	underlying abdominal viscera	1245:1272	underlying abdominal viscera	1245:1272	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	3	104	theme	tissue	612:617	arg1	integration					619:629	tissue integration	612:629	tissue integration	612:629	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	8	105	theme	acellular	1290:1298	arg1	constructs					1300:1309	acellular constructs	1290:1309	acellular constructs	1290:1309	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	8	106	theme	greater	1350:1356	arg1	strength					1370:1377	greater integration strength	1350:1377	greater integration strength	1350:1377	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	7	107	theme	adhesions	1232:1240	arg1	absence					1221:1227	the absence	1217:1227	the absence of adhesions to underlying abdominal viscera	1217:1272	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	7	107	theme	adhesions	1232:1240	arg1	tissue					1175:1180	de novo tissue and vascular network formation	1167:1211	tissue	1175:1180	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	7	107	theme	adhesions	1232:1240	arg1	formation					1203:1211	de novo tissue and vascular network formation	1167:1211	formation	1203:1211	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	9	108	theme	M1	1689:1690	arg1	macrophages					1692:1702	M1 macrophages	1689:1702	M1 macrophages	1689:1702	A decrease in the ratio of M1 macrophages to total macrophages was also observed in hMSC-populated samples.
26283165	11	109	theme	large	1991:1995	arg1	defects					2009:2015	large soft tissue defects	1991:2015	large soft tissue defects caused by trauma or treatment complications	1991:2059	STATEMENT OF SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications represents a critical challenge in the clinic.
26283165	3	110	theme	Collagen	359:366	arg1	type					368:371	Collagen type I	359:373	Collagen type I	359:373	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	12	111	theme	stem	2184:2187	arg1	delivery					2194:2201	stem cell delivery	2184:2201	stem cell delivery	2184:2201	In this study, a novel composite construct was engineered and evaluated for stem cell delivery and tissue repair.
26283165	3	112	dep	micropatterned	485:498	arg1	laser					479:483	laser	479:483	laser	479:483	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	10	113	with	bridge	1851:1856	arg1	capacity					1887:1894	the capacity	1883:1894	the capacity for enhanced tissue repair and integration	1883:1937	Laser micromachined collagen-alginate composites containing hMSCs can be used to bridge soft tissue defects with the capacity for enhanced tissue repair and integration.
26283165	7	114	dep	de	1167:1168	arg1	novo					1170:1173	novo	1170:1173	novo	1170:1173	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
26283165	13	115	theme	tissue	2449:2454	arg1	integration					2456:2466	tissue integration	2449:2466	tissue integration	2449:2466	Laser micromachining was used to fabricate patterned, microporous constructs designed with pores of defined size and distribution as a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration.
26283165	0	116	theme	composite	11:19	arg1	fascia					21:26	composite fascia	11:26	composite fascia	11:26	Engineered composite fascia for stem cell therapy in tissue repair applications.
26283165	12	117	theme	tissue	2207:2212	arg1	repair					2214:2219	tissue repair	2207:2219	tissue repair	2207:2219	In this study, a novel composite construct was engineered and evaluated for stem cell delivery and tissue repair.
26283165	15	118	theme	integration	2814:2824	arg1	strength					2795:2802	the strength	2791:2802	the strength of tissue integration	2791:2824	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	15	119	from	repair	2553:2558	arg1	model					2611:2615	a rat model	2605:2615	a rat model	2605:2615	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	15	120	theme	phenotypic	2694:2703	arg1	response					2705:2712	a reparative phenotypic response	2681:2712	a reparative phenotypic response	2681:2712	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	13	121	theme	mechanical	2371:2380	arg1	responses					2382:2390	mechanical responses	2371:2390	mechanical responses	2371:2390	Laser micromachining was used to fabricate patterned, microporous constructs designed with pores of defined size and distribution as a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration.
26283165	5	122	theme	alginate	840:847	arg1	gel					849:851	an RGD-functionalized alginate gel	818:851	an RGD-functionalized alginate gel modified to degrade in vivo	818:879	hMSCs were loaded in an RGD-functionalized alginate gel modified to degrade in vivo.
26283165	10	123	used	used	1843:1846	arg2	composites					1808:1817	Laser micromachined collagen-alginate composites	1770:1817	Laser micromachined collagen-alginate composites containing hMSCs	1770:1834	Laser micromachined collagen-alginate composites containing hMSCs can be used to bridge soft tissue defects with the capacity for enhanced tissue repair and integration.
26283165	6	124	theme	in	914:915	arg1	viability					934:942	in vitro, high cell viability	914:942	in vitro, high cell viability	914:942	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	13	125	theme	distribution	2339:2350	arg1	pores					2313:2317	pores	2313:2317	pores of defined size and distribution	2313:2350	Laser micromachining was used to fabricate patterned, microporous constructs designed with pores of defined size and distribution as a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration.
26283165	15	126	theme	host	2652:2655	arg1	immunity					2664:2671	host innate immunity	2652:2671	host innate immunity	2652:2671	Upon repair of a full thickness abdominal wall defect in a rat model, the composite construct modulated host innate immunity towards a reparative phenotypic response, promoted neovascularization and associated matrix production, and increased the strength of tissue integration.
26283165	8	127	theme	macrophages	1571:1581	arg1	infiltration					1555:1566	infiltration	1555:1566	infiltration of macrophages	1555:1581	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	10	128	theme	collagen-alginate	1790:1806	arg1	composites					1808:1817	Laser micromachined collagen-alginate composites	1770:1817	Laser micromachined collagen-alginate composites containing hMSCs	1770:1834	Laser micromachined collagen-alginate composites containing hMSCs can be used to bridge soft tissue defects with the capacity for enhanced tissue repair and integration.
26283165	12	129	theme	novel	2125:2129	arg1	construct					2141:2149	a novel composite construct	2123:2149	a novel composite construct	2123:2149	In this study, a novel composite construct was engineered and evaluated for stem cell delivery and tissue repair.
26283165	8	130	dep	increased	1452:1460	arg1	cell					1479:1482	cell	1479:1482	cell seeded	1479:1489	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	6	131	theme	observation	895:905	arg1	period					907:912	a 7 day observation period	887:912	a 7 day observation period	887:912	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	3	132	theme	laminated	394:402	arg1	sheets					413:418	laminated collagen sheets	394:418	laminated collagen sheets	394:418	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	8	133	theme	0.59	1425:1428	arg1	±					1430:1430	±	1430:1430	±	1430:1430	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	1	134	from	challenge	92:100	arg1	regeneration					112:123	tissue regeneration	105:123	tissue regeneration	105:123	A critical challenge in tissue regeneration is to develop constructs that effectively integrate with the host tissue.
26283165	6	135	theme	7	889:889	arg1	day					891:893	day	891:893	day	891:893	Over a 7 day observation period in vitro, high cell viability was observed with constant levels of VEGF, PDGF-β and MCP-1 protein expression.
26283165	8	136	dep	displayed	1340:1348	arg1	cell					1380:1383	cell	1380:1383	cell seeded	1380:1390	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	11	137	theme	SIGNIFICANCE	1953:1964	arg1	restoration					1976:1986	SIGNIFICANCE Effective restoration	1953:1986	SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications	1953:2059	STATEMENT OF SIGNIFICANCE Effective restoration of large soft tissue defects caused by trauma or treatment complications represents a critical challenge in the clinic.
26283165	8	138	theme	0.25	1432:1435	arg1	±					1430:1430	±	1430:1430	±	1430:1430	As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength (cell seeded: 0.92 ± 0.19 N/mm vs. acellular: 0.59 ± 0.25 N/mm, p=0.01), increased vascularization (cell seeded: 2.7-2.1/hpf vs. acellular: 1.7-2.1/hpf, p<0.03), and increased infiltration of macrophages (cell seeded: 2021-3630 μm(2)/hpf vs. acellular: 1570-2530 μm(2)/hpf, p<0.05).
26283165	3	139	theme	dimension	522:530	arg1	pores					505:509	pores	505:509	pores of defined dimension and spatial distribution	505:555	Collagen type I was fashioned into laminated collagen sheets to form a mechanically robust fascia that was subsequently laser micropatterned with pores of defined dimension and spatial distribution as a means to modulate mechanical behavior and promote tissue integration.
26283165	9	140	theme	hMSC-populated	1746:1759	arg1	samples					1761:1767	hMSC-populated samples	1746:1767	hMSC-populated samples	1746:1767	A decrease in the ratio of M1 macrophages to total macrophages was also observed in hMSC-populated samples.
26283165	13	141	theme	size	2330:2333	arg1	pores					2313:2317	pores	2313:2317	pores of defined size and distribution	2313:2350	Laser micromachining was used to fabricate patterned, microporous constructs designed with pores of defined size and distribution as a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration.
26283165	10	142	contain	containing	1819:1828	arg1	composites					1808:1817	Laser micromachined collagen-alginate composites	1770:1817	Laser micromachined collagen-alginate composites containing hMSCs	1770:1834	Laser micromachined collagen-alginate composites containing hMSCs can be used to bridge soft tissue defects with the capacity for enhanced tissue repair and integration.
26283165	10	142	contain	containing	1819:1828	arg2	hMSCs					1830:1834	hMSCs	1830:1834	hMSCs	1830:1834	Laser micromachined collagen-alginate composites containing hMSCs can be used to bridge soft tissue defects with the capacity for enhanced tissue repair and integration.
26283165	10	143	theme	tissue	1863:1868	arg1	defects					1870:1876	soft tissue defects	1858:1876	soft tissue defects	1858:1876	Laser micromachined collagen-alginate composites containing hMSCs can be used to bridge soft tissue defects with the capacity for enhanced tissue repair and integration.
26283165	13	144	dep	patterned	2265:2273	arg1	microporous					2276:2286	microporous	2276:2286	microporous	2276:2286	Laser micromachining was used to fabricate patterned, microporous constructs designed with pores of defined size and distribution as a means to tune mechanical responses, accommodate and protect incorporated cells, and enhance tissue integration.
26283165	7	145	theme	network	1195:1201	arg1	formation					1203:1211	de novo tissue and vascular network formation	1167:1211	formation	1203:1211	In a full thickness abdominal wall defect model, the composite construct prevented hernia recurrence in Wistar rats over an 8-week period with de novo tissue and vascular network formation and the absence of adhesions to underlying abdominal viscera.
25522372	5	0	theme	biofilm	821:827	arg1	strains					837:843	biofilm forming strains	821:843	biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa	821:895	Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa.
25522372	1	1	dep	describes	105:113	arg1	films					204:208	films	204:208	films exhibiting high antibacterial activity	204:247	This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity.
25522372	1	1	dep	describes	105:113	arg1	either					181:186	either	181:186	either	181:186	This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity.
25522372	5	2	theme	antibacterial	790:802	arg1	activity					804:811	the highest antibacterial activity	778:811	the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa	778:895	Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa.
25522372	5	3	theme	forming	829:835	arg1	strains					837:843	biofilm forming strains	821:843	biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa	821:895	Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa.
25522372	2	4	from	embedded	386:393	arg1	it					398:399	it	398:399	it	398:399	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	6	5	theme	tumoral	987:993	arg1	cells					995:999	mammalian somatic and tumoral cells	965:999	mammalian somatic and tumoral cells	965:999	The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.
25522372	3	6	theme	precursor	565:573	arg1	concentration					575:587	an optimized gold precursor concentration	547:587	an optimized gold precursor concentration	547:587	Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration.
25522372	2	7	from	it	398:399	arg1	embedded					386:393	embedded	386:393	embedded	386:393	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	1	8	theme	nanocomposites	166:179	arg1	characterization					133:148	characterization	133:148	characterization	133:148	This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity.
25522372	1	8	theme	nanocomposites	166:179	arg1	synthesis					119:127	synthesis	119:127	synthesis	119:127	This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity.
25522372	5	9	theme	highest	782:788	arg1	activity					804:811	the highest antibacterial activity	778:811	the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa	778:895	Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa.
25522372	6	10	theme	somatic	975:981	arg1	cells					995:999	mammalian somatic and tumoral cells	965:999	mammalian somatic and tumoral cells	965:999	The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.
25522372	4	11	theme	cytotoxic	661:669	arg1	materials					685:693	cytotoxic nanocomposite materials	661:693	antimicrobial but not cytotoxic nanocomposite materials	639:693	Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials.
25522372	2	12	theme	stabilizing	328:338	arg1	agent					340:344	reducing and stabilizing agent	315:344	agent	340:344	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	2	12	theme	stabilizing	328:338	arg1	chitosan					294:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	2	13	used	used	307:310	arg2	chitosan					294:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	2	13	used	used	307:310	arg2	agent					340:344	reducing and stabilizing agent	315:344	agent	340:344	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	3	14	theme	different	436:444	arg1	grades					455:460	three different chitosan grades	430:460	three different chitosan grades varying in the average molecular weight and deacetylation degree (DD)	430:530	Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration.
25522372	6	15	theme	mammalian	965:973	arg1	cells					995:999	mammalian somatic and tumoral cells	965:999	mammalian somatic and tumoral cells	965:999	The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.
25522372	7	16	theme	disruptive	1018:1027	arg1	effect					1029:1034	a disruptive effect	1016:1034	a disruptive effect on the bacteria wall	1016:1055	They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.
25522372	3	17	theme	gold	560:563	arg1	concentration					575:587	an optimized gold precursor concentration	547:587	an optimized gold precursor concentration	547:587	Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration.
25522372	3	18	theme	average	477:483	arg1	weight					495:500	the average molecular weight	473:500	the average molecular weight	473:500	Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration.
25522372	8	19	from	coatings	1201:1208	arg1	field					1228:1232	the biomedical field	1213:1232	the biomedical field	1213:1232	This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.
25522372	2	20	theme	gold	367:370	arg1	nanoparticles					372:384	gold nanoparticles	367:384	gold nanoparticles embedded in it	367:399	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	1	21	theme	high	221:224	arg1	activity					240:247	high antibacterial activity	221:247	high antibacterial activity	221:247	This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity.
25522372	8	22	theme	antimicrobial	1187:1199	arg1	coatings					1201:1208	antimicrobial coatings	1187:1208	antimicrobial coatings in the biomedical field	1187:1232	This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.
25522372	4	23	theme	Several	590:596	arg1	factors					598:604	Several factors	590:604	Several factors	590:604	Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials.
25522372	1	24	theme	antibacterial	226:238	arg1	activity					240:247	high antibacterial activity	221:247	high antibacterial activity	221:247	This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity.
25522372	0	25	theme	chitosan-gold	28:40	arg1	Development					0:10	Development	0:10	Development of noncytotoxic chitosan-gold	0:40	Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.
25522372	1	26	dep	either	181:186	arg1	colloidal					188:196	colloidal	188:196	colloidal	188:196	This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity.
25522372	7	27	theme	bacteria	1043:1050	arg1	wall					1052:1055	the bacteria wall	1039:1055	the bacteria wall	1039:1055	They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.
25522372	4	28	theme	nanocomposite	671:683	arg1	materials					685:693	cytotoxic nanocomposite materials	661:693	antimicrobial but not cytotoxic nanocomposite materials	639:693	Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials.
25522372	0	29	theme	noncytotoxic	15:26	arg1	chitosan-gold					28:40	noncytotoxic chitosan-gold	15:40	noncytotoxic chitosan-gold	15:40	Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.
25522372	3	30	theme	optimized	550:558	arg1	concentration					575:587	an optimized gold precursor concentration	547:587	an optimized gold precursor concentration	547:587	Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration.
25522372	3	31	theme	deacetylation	506:518	arg1	DD					528:529	DD	528:529	DD	528:529	Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration.
25522372	3	31	theme	deacetylation	506:518	arg1	degree					520:525	deacetylation degree	506:525	deacetylation degree (DD)	506:530	Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration.
25522372	3	32	theme	chitosan	446:453	arg1	grades					455:460	three different chitosan grades	430:460	three different chitosan grades varying in the average molecular weight and deacetylation degree (DD)	430:530	Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration.
25522372	5	33	theme	highest	757:763	arg1	DD					765:766	the highest DD	753:766	the highest DD	753:766	Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa.
25522372	3	34	theme	molecular	485:493	arg1	weight					495:500	the average molecular weight	473:500	the average molecular weight	473:500	Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration.
25522372	8	35	theme	biomedical	1217:1226	arg1	field					1228:1232	the biomedical field	1213:1232	the biomedical field	1213:1232	This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.
25522372	2	36	theme	polymer	286:292	arg1	agent					340:344	reducing and stabilizing agent	315:344	agent	340:344	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	2	36	theme	polymer	286:292	arg1	chitosan					294:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	5	37	theme	aeruginosa	886:895	arg1	strains					837:843	biofilm forming strains	821:843	biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa	821:895	Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa.
25522372	0	38	theme	antibacterial	70:82	arg1	materials					84:92	efficient antibacterial materials	60:92	efficient antibacterial materials	60:92	Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.
25522372	5	39	theme	aureus	863:868	arg1	strains					837:843	biofilm forming strains	821:843	biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa	821:895	Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa.
25522372	2	40	theme	biodegradable	272:284	arg1	agent					340:344	reducing and stabilizing agent	315:344	agent	340:344	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	2	40	theme	biodegradable	272:284	arg1	chitosan					294:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	7	41	theme	eukaryotic	1105:1114	arg1	cells					1116:1120	the eukaryotic cells	1101:1120	the eukaryotic cells	1101:1120	They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.
25522372	0	42	theme	efficient	60:68	arg1	materials					84:92	efficient antibacterial materials	60:92	efficient antibacterial materials	60:92	Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.
25522372	5	43	theme	medium	725:730	arg1	weight					742:747	medium molecular weight	725:747	medium molecular weight	725:747	Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa.
25522372	2	44	theme	biocompatible	254:266	arg1	agent					340:344	reducing and stabilizing agent	315:344	agent	340:344	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	2	44	theme	biocompatible	254:266	arg1	chitosan					294:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	7	45	from	effect	1029:1034	arg1	wall					1052:1055	the bacteria wall	1039:1055	the bacteria wall	1039:1055	They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.
25522372	5	46	theme	molecular	732:740	arg1	weight					742:747	medium molecular weight	725:747	medium molecular weight	725:747	Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa.
25522372	3	47	used	used	537:540	arg2	grades					455:460	three different chitosan grades	430:460	three different chitosan grades varying in the average molecular weight and deacetylation degree (DD)	430:530	Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration.
25522372	2	48	theme	reducing	315:322	arg1	agent					340:344	reducing and stabilizing agent	315:344	agent	340:344	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	2	48	theme	reducing	315:322	arg1	chitosan					294:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan	250:301	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	1	49	theme	noncytotoxic	153:164	arg1	nanocomposites					166:179	noncytotoxic nanocomposites	153:179	noncytotoxic nanocomposites	153:179	This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity.
25522372	6	50	theme	resulting	902:910	arg1	nanocomposites					912:925	The resulting nanocomposites	898:925	The resulting nanocomposites	898:925	The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.
25522372	2	51	theme	nanoparticles	372:384	arg1	synthesis					354:362	the synthesis	350:362	the synthesis of gold nanoparticles embedded in it	350:399	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	2	52	theme	embedded	386:393	arg1	nanoparticles					372:384	gold nanoparticles	367:384	gold nanoparticles embedded in it	367:399	The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it.
25522372	1	53	dep	synthesis	119:127	arg1	the					115:117	the	115:117	the	115:117	This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity.
25522372	3	54	theme	first	418:422	arg1	time					424:427	the first time	414:427	the first time	414:427	Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration.
25522372	5	55	with	chitosan	711:718	arg1	weight					742:747	medium molecular weight	725:747	medium molecular weight	725:747	Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa.
25522372	5	55	with	chitosan	711:718	arg1	DD					765:766	the highest DD	753:766	the highest DD	753:766	Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa.
25924866	6	0	dep	6'-Sialyl-Hex-Ac-HexNAc	847:869	arg1	isomer					927:932	isomer	927:932	isomer not further specified	927:954	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	6	1	theme	equine	704:709	arg1	literature					728:737	equine oligosaccharides literature	704:737	equine oligosaccharides literature	704:737	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	8	2	theme	specific	1264:1271	arg1	EMOS					1273:1276	specific EMOS	1264:1276	specific EMOS	1264:1276	Differences in presence and in abundance of specific EMOS were evident not only between the four breeds but also within the breed.
25924866	0	3	theme	breeds	76:81	arg1	colostrum					47:55	colostrum	47:55	colostrum of different horse breeds	47:81	Comparison of milk oligosaccharides pattern in colostrum of different horse breeds.
25924866	4	4	theme	which	515:519	arg1	neutral					531:537	neutral	531:537	neutral	531:537	Sixteen EMOS were characterized and quantified, of which half were neutral and half were acidic.
25924866	7	5	with	3'-sialyllactose	1092:1107	arg1	galactosyllactose					1073:1089	3'- galactosyllactose	1069:1089	3'- galactosyllactose	1069:1089	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	7	5	with	3'-sialyllactose	1092:1107	arg1	β					1061:1061	β 6'	1061:1064	β 6'	1061:1064	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	7	6	dep	ranged	1026:1031	arg1	%					1202:1202	27-59, 16-37, 1-8, and 1-6%	1176:1202	27-59, 16-37, 1-8, and 1-6%	1176:1202	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	7	7	theme	3'-	1069:1071	arg1	galactosyllactose					1073:1089	3'- galactosyllactose	1069:1089	3'- galactosyllactose	1069:1089	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	7	8	theme	colostrum	965:973	arg1	samples					975:981	all colostrum samples	961:981	all colostrum samples	961:981	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	2	9	theme	Oligosaccharide	175:189	arg1	composition					191:201	Oligosaccharide composition	175:201	Oligosaccharide composition	175:201	Oligosaccharide composition is species-specific, and equine colostrum has been reported to contain unique oligosaccharides.
25924866	6	10	theme	β1-4	769:772	arg1	Hex-Hex					774:780	Gal(β1-4)Hex-Hex	765:780	Gal(β1-4)Hex-Hex	765:780	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	8	11	theme	EMOS	1273:1276	arg1	abundance					1251:1259	abundance	1251:1259	abundance of specific EMOS	1251:1276	Differences in presence and in abundance of specific EMOS were evident not only between the four breeds but also within the breed.
25924866	5	12	theme	human	607:611	arg1	oligosaccharides					618:633	human milk oligosaccharides	607:633	human milk oligosaccharides	607:633	EMOS showed about 63% structural overlap with human milk oligosaccharides, known for their bioactivity.
25924866	8	13	from	Differences	1220:1230	arg1	presence					1235:1242	presence	1235:1242	presence	1235:1242	Differences in presence and in abundance of specific EMOS were evident not only between the four breeds but also within the breed.
25924866	8	13	from	Differences	1220:1230	arg1	abundance					1251:1259	abundance	1251:1259	abundance of specific EMOS	1251:1276	Differences in presence and in abundance of specific EMOS were evident not only between the four breeds but also within the breed.
25924866	7	14	with	disialyllactose	1114:1128	arg1	galactosyllactose					1073:1089	3'- galactosyllactose	1069:1089	3'- galactosyllactose	1069:1089	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	7	14	with	disialyllactose	1114:1128	arg1	β					1061:1061	β 6'	1061:1064	β 6'	1061:1064	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	7	15	theme	oligosaccharides	1158:1173	arg1	abundant					1142:1149	abundant	1142:1149	abundant	1142:1149	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	6	16	theme	acidic	840:845	arg1	6'-Sialyl-Hex-Ac-HexNAc					847:869	acidic 6'-Sialyl-Hex-Ac-HexNAc	840:869	acidic 6'-Sialyl-Hex-Ac-HexNAc	840:869	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	5	17	theme	milk	613:616	arg1	oligosaccharides					618:633	human milk oligosaccharides	607:633	human milk oligosaccharides	607:633	EMOS showed about 63% structural overlap with human milk oligosaccharides, known for their bioactivity.
25924866	6	18	theme	Gal	765:767	arg1	Hex-Hex					774:780	Gal(β1-4)Hex-Hex	765:780	Gal(β1-4)Hex-Hex	765:780	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	0	19	from	pattern	36:42	arg1	colostrum					47:55	colostrum	47:55	colostrum of different horse breeds	47:81	Comparison of milk oligosaccharides pattern in colostrum of different horse breeds.
25924866	0	20	theme	horse	70:74	arg1	breeds					76:81	different horse breeds	60:81	different horse breeds	60:81	Comparison of milk oligosaccharides pattern in colostrum of different horse breeds.
25924866	1	21	theme	Colostrum	84:92	arg1	oligosaccharides					94:109	Colostrum oligosaccharides	84:109	Colostrum oligosaccharides	84:109	Colostrum oligosaccharides are known to exhibit prebiotic and immunomodulatory properties.
25924866	4	22	dep	characterized	482:494	arg1	neutral					531:537	neutral	531:537	neutral	531:537	Sixteen EMOS were characterized and quantified, of which half were neutral and half were acidic.
25924866	4	22	dep	characterized	482:494	arg1	acidic					553:558	acidic	553:558	acidic	553:558	Sixteen EMOS were characterized and quantified, of which half were neutral and half were acidic.
25924866	0	23	theme	milk	14:17	arg1	Comparison					0:9	Comparison	0:9	Comparison of milk	0:17	Comparison of milk oligosaccharides pattern in colostrum of different horse breeds.
25924866	7	24	with	g/L	1051:1053	arg1	galactosyllactose					1073:1089	3'- galactosyllactose	1069:1089	3'- galactosyllactose	1069:1089	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	7	24	with	g/L	1051:1053	arg1	β					1061:1061	β 6'	1061:1064	β 6'	1061:1064	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	5	25	dep	oligosaccharides	618:633	arg1	overlap					594:600	overlap	594:600	overlap	594:600	EMOS showed about 63% structural overlap with human milk oligosaccharides, known for their bioactivity.
25924866	7	26	dep	4.63	1046:1049	arg1	to					1043:1044	to	1043:1044	to	1043:1044	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	3	27	theme	exoglycosidase	436:449	arg1	degradation					451:461	exoglycosidase degradation	436:461	exoglycosidase degradation	436:461	Therefore, equine oligosaccharides (EMOS) from colostrum from different horse breeds were analyzed by CE-LIF, CE-MS(n), HILIC-MS(n), and exoglycosidase degradation.
25924866	3	28	theme	equine	310:315	arg1	oligosaccharides					317:332	equine oligosaccharides	310:332	equine oligosaccharides (EMOS) from colostrum from different horse breeds	310:382	Therefore, equine oligosaccharides (EMOS) from colostrum from different horse breeds were analyzed by CE-LIF, CE-MS(n), HILIC-MS(n), and exoglycosidase degradation.
25924866	3	28	theme	equine	310:315	arg1	EMOS					335:338	EMOS	335:338	EMOS	335:338	Therefore, equine oligosaccharides (EMOS) from colostrum from different horse breeds were analyzed by CE-LIF, CE-MS(n), HILIC-MS(n), and exoglycosidase degradation.
25924866	6	29	theme	β1-4	752:755	arg1	HexNAc					757:762	neutral Gal(β1-4)HexNAc	740:762	neutral Gal(β1-4)HexNAc	740:762	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	6	30	theme	Gal	748:750	arg1	HexNAc					757:762	neutral Gal(β1-4)HexNAc	740:762	neutral Gal(β1-4)HexNAc	740:762	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	6	31	dep	reported	685:692	arg1	6'-Sialyl-Hex-Ac-HexNAc					847:869	acidic 6'-Sialyl-Hex-Ac-HexNAc	840:869	acidic 6'-Sialyl-Hex-Ac-HexNAc	840:869	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	6	31	dep	reported	685:692	arg1	β4'-galactosyllactose					783:803	β4'-galactosyllactose	783:803	β4'-galactosyllactose	783:803	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	6	31	dep	reported	685:692	arg1	HexNAc					757:762	neutral Gal(β1-4)HexNAc	740:762	neutral Gal(β1-4)HexNAc	740:762	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	6	31	dep	reported	685:692	arg1	lactose-N-hexaose					810:826	lactose-N-hexaose	810:826	lactose-N-hexaose	810:826	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	6	31	dep	reported	685:692	arg1	Hex-Hex					774:780	Gal(β1-4)Hex-Hex	765:780	Gal(β1-4)Hex-Hex	765:780	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	6	32	theme	neutral	740:746	arg1	HexNAc					757:762	neutral Gal(β1-4)HexNAc	740:762	neutral Gal(β1-4)HexNAc	740:762	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	1	33	theme	prebiotic	132:140	arg1	properties					163:172	prebiotic and immunomodulatory properties	132:172	prebiotic and immunomodulatory properties	132:172	Colostrum oligosaccharides are known to exhibit prebiotic and immunomodulatory properties.
25924866	7	34	theme	average	988:994	arg1	concentration					1012:1024	the average oligosaccharide concentration	984:1024	the average oligosaccharide concentration	984:1024	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	2	35	theme	equine	228:233	arg1	colostrum					235:243	equine colostrum	228:243	equine colostrum	228:243	Oligosaccharide composition is species-specific, and equine colostrum has been reported to contain unique oligosaccharides.
25924866	7	36	theme	oligosaccharide	996:1010	arg1	concentration					1012:1024	the average oligosaccharide concentration	984:1024	the average oligosaccharide concentration	984:1024	In all colostrum samples, the average oligosaccharide concentration ranged from 2.12 to 4.63 g/L; with β 6'and 3'- galactosyllactose, 3'-sialyllactose, and disialyllactose as the most abundant of all oligosaccharides (27-59, 16-37, 1-8, and 1-6%, respectively).
25924866	3	37	theme	horse	371:375	arg1	breeds					377:382	different horse breeds	361:382	different horse breeds	361:382	Therefore, equine oligosaccharides (EMOS) from colostrum from different horse breeds were analyzed by CE-LIF, CE-MS(n), HILIC-MS(n), and exoglycosidase degradation.
25924866	3	38	from	breeds	377:382	arg1	oligosaccharides					317:332	equine oligosaccharides	310:332	equine oligosaccharides (EMOS) from colostrum from different horse breeds	310:382	Therefore, equine oligosaccharides (EMOS) from colostrum from different horse breeds were analyzed by CE-LIF, CE-MS(n), HILIC-MS(n), and exoglycosidase degradation.
25924866	3	38	from	breeds	377:382	arg1	EMOS					335:338	EMOS	335:338	EMOS	335:338	Therefore, equine oligosaccharides (EMOS) from colostrum from different horse breeds were analyzed by CE-LIF, CE-MS(n), HILIC-MS(n), and exoglycosidase degradation.
25924866	3	39	theme	different	361:369	arg1	breeds					377:382	different horse breeds	361:382	different horse breeds	361:382	Therefore, equine oligosaccharides (EMOS) from colostrum from different horse breeds were analyzed by CE-LIF, CE-MS(n), HILIC-MS(n), and exoglycosidase degradation.
25924866	1	40	theme	immunomodulatory	146:161	arg1	properties					163:172	prebiotic and immunomodulatory properties	132:172	prebiotic and immunomodulatory properties	132:172	Colostrum oligosaccharides are known to exhibit prebiotic and immunomodulatory properties.
25924866	2	41	contain	contain	266:272	arg2	oligosaccharides					281:296	unique oligosaccharides	274:296	unique oligosaccharides	274:296	Oligosaccharide composition is species-specific, and equine colostrum has been reported to contain unique oligosaccharides.
25924866	2	41	contain	contain	266:272	arg1	colostrum					235:243	equine colostrum	228:243	equine colostrum	228:243	Oligosaccharide composition is species-specific, and equine colostrum has been reported to contain unique oligosaccharides.
25924866	0	42	theme	different	60:68	arg1	breeds					76:81	different horse breeds	60:81	different horse breeds	60:81	Comparison of milk oligosaccharides pattern in colostrum of different horse breeds.
25924866	2	43	theme	unique	274:279	arg1	oligosaccharides					281:296	unique oligosaccharides	274:296	unique oligosaccharides	274:296	Oligosaccharide composition is species-specific, and equine colostrum has been reported to contain unique oligosaccharides.
25924866	6	44	theme	oligosaccharides	711:726	arg1	literature					728:737	equine oligosaccharides literature	704:737	equine oligosaccharides literature	704:737	Seven EMOS were not reported before in equine oligosaccharides literature: neutral Gal(β1-4)HexNAc, Gal(β1-4)Hex-Hex, β4'-galactosyllactose, and lactose-N-hexaose, as well as acidic 6'-Sialyl-Hex-Ac-HexNAc, sialyllacto-N-tetraose-a, and disialylacto-N-tetraose (isomer not further specified).
25924866	3	45	from	colostrum	346:354	arg1	oligosaccharides					317:332	equine oligosaccharides	310:332	equine oligosaccharides (EMOS) from colostrum from different horse breeds	310:382	Therefore, equine oligosaccharides (EMOS) from colostrum from different horse breeds were analyzed by CE-LIF, CE-MS(n), HILIC-MS(n), and exoglycosidase degradation.
25924866	3	45	from	colostrum	346:354	arg1	EMOS					335:338	EMOS	335:338	EMOS	335:338	Therefore, equine oligosaccharides (EMOS) from colostrum from different horse breeds were analyzed by CE-LIF, CE-MS(n), HILIC-MS(n), and exoglycosidase degradation.
25413790	6	0	dep	sugarcane	1109:1117	arg1	the					1105:1107	the	1105:1107	the	1105:1107	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	0	dep	sugarcane	1109:1117	arg1	ash					1128:1130	ash	1128:1130	ash	1128:1130	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	3	1	theme	620	732:734	arg1	-1					739:740	-1	739:740	-1	739:740	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	3	1	theme	620	732:734	arg1	cm					736:737	1100, 820 and 620 cm	718:737	cm	736:737	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	0	2	theme	possible	74:81	arg1	source					83:88	a possible source	72:88	a possible source of silica	72:98	Carbonization on combustion and biodegradation of agricultural waste as a possible source of silica.
25413790	4	3	from	composition	767:777	arg1	ash					796:798	ash	796:798	ash	796:798	The elemental composition of the silica in ash is determined by EDX analysis.
25413790	3	4	theme	FT-IR	746:750	arg1	-1					739:740	-1	739:740	-1	739:740	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	3	4	theme	FT-IR	746:750	arg1	cm					736:737	1100, 820 and 620 cm	718:737	cm	736:737	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	6	5	from	present	1094:1100	arg1	husk					1123:1126	husk	1123:1126	husk	1123:1126	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	5	from	present	1094:1100	arg1	sugarcane					1109:1117	sugarcane	1109:1117	sugarcane	1109:1117	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	0	6	theme	silica	93:98	arg1	source					83:88	a possible source	72:88	a possible source of silica	72:98	Carbonization on combustion and biodegradation of agricultural waste as a possible source of silica.
25413790	3	7	from	cm	736:737	arg1	peaks					709:713	the peaks	705:713	the peaks at 1100, 820 and 620 cm(-1) of FT-IR	705:750	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	5	8	located	observed	873:880	arg2	reaction					846:853	The exothermic reaction	831:853	The exothermic reaction	831:853	The exothermic reaction and the mass loss observed in the TG/DTG at the transient temperature of 840-850 °C has confirmed the presence of the α-quartz.
25413790	5	8	located	observed	873:880	arg1	TG/DTG					889:894	the TG/DTG	885:894	the TG/DTG at the transient temperature of 840-850 °C	885:937	The exothermic reaction and the mass loss observed in the TG/DTG at the transient temperature of 840-850 °C has confirmed the presence of the α-quartz.
25413790	2	9	theme	EDX	488:490	arg1	techniques					513:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	Presence of silica in the ash samples has been well characterized by the XRD, FT-IR, EDX, SEM and N2 sorption techniques.
25413790	6	10	theme	silica	1033:1038	arg1	presence					1021:1028	the presence	1017:1028	the presence of silica	1017:1038	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	5	11	theme	exothermic	835:844	arg1	reaction					846:853	The exothermic reaction	831:853	The exothermic reaction	831:853	The exothermic reaction and the mass loss observed in the TG/DTG at the transient temperature of 840-850 °C has confirmed the presence of the α-quartz.
25413790	7	12	theme	%	1302:1302	arg1	maximum					1283:1289	a maximum	1281:1289	a maximum of 18.7-52 % and on biodegradation to about 48.3-92.4 %	1281:1345	The study has clearly revealed that the silica content has increased with the increase in temperature and refinement of the combustion condition to a maximum of 18.7-52 % and on biodegradation to about 48.3-92.4 %.
25413790	1	13	from	waste	142:146	arg1	environment					155:165	the environment	151:165	the environment	151:165	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	7	14	theme	combustion	1257:1266	arg1	condition					1268:1276	the combustion condition	1253:1276	the combustion condition	1253:1276	The study has clearly revealed that the silica content has increased with the increase in temperature and refinement of the combustion condition to a maximum of 18.7-52 % and on biodegradation to about 48.3-92.4 %.
25413790	6	15	theme	SEM	983:985	arg1	micrograph					987:996	SEM micrograph	983:996	SEM micrograph	983:996	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	2	16	theme	FT-IR	481:485	arg1	techniques					513:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	Presence of silica in the ash samples has been well characterized by the XRD, FT-IR, EDX, SEM and N2 sorption techniques.
25413790	3	17	theme	=	659:659	arg1	2θ					656:657	2θ	656:657	2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR	656:750	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	7	18	theme	condition	1268:1276	arg1	refinement					1239:1248	refinement	1239:1248	refinement	1239:1248	The study has clearly revealed that the silica content has increased with the increase in temperature and refinement of the combustion condition to a maximum of 18.7-52 % and on biodegradation to about 48.3-92.4 %.
25413790	7	18	theme	condition	1268:1276	arg1	temperature					1223:1233	temperature	1223:1233	temperature	1223:1233	The study has clearly revealed that the silica content has increased with the increase in temperature and refinement of the combustion condition to a maximum of 18.7-52 % and on biodegradation to about 48.3-92.4 %.
25413790	6	19	attach	present	1094:1100	arg1	husk					1123:1126	husk	1123:1126	husk	1123:1126	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	19	attach	present	1094:1100	arg2	shapes					1077:1082	the various crystal shapes	1057:1082	the various crystal shapes that were present in the sugarcane and husk ash	1057:1130	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	19	attach	present	1094:1100	arg1	sugarcane					1109:1117	sugarcane	1109:1117	sugarcane	1109:1117	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	19	attach	present	1094:1100	arg2	present					1094:1100	present	1094:1100	present	1094:1100	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	7	20	theme	silica	1173:1178	arg1	content					1180:1186	the silica content	1169:1186	the silica content	1169:1186	The study has clearly revealed that the silica content has increased with the increase in temperature and refinement of the combustion condition to a maximum of 18.7-52 % and on biodegradation to about 48.3-92.4 %.
25413790	7	21	from	maximum	1283:1289	arg1	biodegradation					1311:1324	biodegradation	1311:1324	biodegradation to about 48.3-92.4 %	1311:1345	The study has clearly revealed that the silica content has increased with the increase in temperature and refinement of the combustion condition to a maximum of 18.7-52 % and on biodegradation to about 48.3-92.4 %.
25413790	1	22	theme	different	354:362	arg1	conditions					372:381	different thermal conditions	354:381	different thermal conditions	354:381	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	4	23	theme	EDX	817:819	arg1	analysis					821:828	EDX analysis	817:828	EDX analysis	817:828	The elemental composition of the silica in ash is determined by EDX analysis.
25413790	2	24	theme	ash	429:431	arg1	samples					433:439	the ash samples	425:439	the ash samples	425:439	Presence of silica in the ash samples has been well characterized by the XRD, FT-IR, EDX, SEM and N2 sorption techniques.
25413790	1	25	theme	agricultural	256:267	arg1	waste					269:273	the agricultural waste	252:273	the agricultural waste	252:273	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	3	26	theme	X-ray	615:619	arg1	peaks					633:637	the X-ray diffraction peaks	611:637	the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR	611:750	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	2	27	theme	sorption	504:511	arg1	techniques					513:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	Presence of silica in the ash samples has been well characterized by the XRD, FT-IR, EDX, SEM and N2 sorption techniques.
25413790	6	28	theme	crystal	1069:1075	arg1	shapes					1077:1082	the various crystal shapes	1057:1082	the various crystal shapes that were present in the sugarcane and husk ash	1057:1130	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	28	theme	crystal	1069:1075	arg1	present					1094:1100	present	1094:1100	present	1094:1100	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	7	29	from	increase	1211:1218	arg1	refinement					1239:1248	refinement	1239:1248	refinement	1239:1248	The study has clearly revealed that the silica content has increased with the increase in temperature and refinement of the combustion condition to a maximum of 18.7-52 % and on biodegradation to about 48.3-92.4 %.
25413790	7	29	from	increase	1211:1218	arg1	temperature					1223:1233	temperature	1223:1233	temperature	1223:1233	The study has clearly revealed that the silica content has increased with the increase in temperature and refinement of the combustion condition to a maximum of 18.7-52 % and on biodegradation to about 48.3-92.4 %.
25413790	2	30	theme	silica	415:420	arg1	Presence					403:410	Presence	403:410	Presence of silica in the ash samples	403:439	Presence of silica in the ash samples has been well characterized by the XRD, FT-IR, EDX, SEM and N2 sorption techniques.
25413790	2	31	theme	N2	501:502	arg1	techniques					513:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	Presence of silica in the ash samples has been well characterized by the XRD, FT-IR, EDX, SEM and N2 sorption techniques.
25413790	1	32	theme	thermal	364:370	arg1	conditions					372:381	different thermal conditions	354:381	different thermal conditions	354:381	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	1	33	theme	Agricultural	101:112	arg1	waste					142:146	the major solid waste	126:146	the major solid waste in the environment	126:165	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	1	33	theme	Agricultural	101:112	arg1	waste					114:118	Agricultural waste	101:118	Agricultural waste	101:118	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	6	34	from	sugarcane	1109:1117	arg1	shapes					1077:1082	the various crystal shapes	1057:1082	the various crystal shapes that were present in the sugarcane and husk ash	1057:1130	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	34	from	sugarcane	1109:1117	arg1	present					1094:1100	present	1094:1100	present	1094:1100	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	1	35	theme	sugarcane	280:288	arg1	ash					298:300	sugarcane bagasse ash	280:300	sugarcane bagasse ash	280:300	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	5	36	theme	°C	936:937	arg1	temperature					913:923	the transient temperature	899:923	the transient temperature of 840-850 °C	899:937	The exothermic reaction and the mass loss observed in the TG/DTG at the transient temperature of 840-850 °C has confirmed the presence of the α-quartz.
25413790	1	37	attach	presence	210:217	arg1	combustion					340:349	combustion	340:349	combustion	340:349	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	1	37	attach	presence	210:217	arg2	minerals					222:229	minerals	222:229	minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization	222:335	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	1	38	theme	bagasse	290:296	arg1	ash					298:300	sugarcane bagasse ash	280:300	sugarcane bagasse ash	280:300	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	1	39	from	silica	242:247	arg1	waste					269:273	the agricultural waste	252:273	the agricultural waste	252:273	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	3	40	theme	silica	555:560	arg1	phases					545:550	crystal phases	537:550	crystal phases of silica	537:560	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	2	41	theme	SEM	493:495	arg1	techniques					513:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	Presence of silica in the ash samples has been well characterized by the XRD, FT-IR, EDX, SEM and N2 sorption techniques.
25413790	0	42	theme	waste	63:67	arg1	combustion					17:26	combustion	17:26	combustion	17:26	Carbonization on combustion and biodegradation of agricultural waste as a possible source of silica.
25413790	0	42	theme	waste	63:67	arg1	biodegradation					32:45	biodegradation	32:45	biodegradation	32:45	Carbonization on combustion and biodegradation of agricultural waste as a possible source of silica.
25413790	3	43	dep	=	659:659	arg1	27					661:662	27	661:662	27	661:662	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	3	43	dep	=	659:659	arg1	60					672:673	60	672:673	60	672:673	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	3	43	dep	=	659:659	arg1	40					665:666	40	665:666	40	665:666	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	3	44	theme	crystal	537:543	arg1	phases					545:550	crystal phases	537:550	crystal phases of silica	537:560	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	0	45	theme	agricultural	50:61	arg1	waste					63:67	agricultural waste	50:67	agricultural waste	50:67	Carbonization on combustion and biodegradation of agricultural waste as a possible source of silica.
25413790	5	46	theme	α-quartz	973:980	arg1	presence					957:964	the presence	953:964	the presence of the α-quartz	953:980	The exothermic reaction and the mass loss observed in the TG/DTG at the transient temperature of 840-850 °C has confirmed the presence of the α-quartz.
25413790	3	47	theme	diffraction	621:631	arg1	peaks					633:637	the X-ray diffraction peaks	611:637	the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR	611:750	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	3	48	theme	phases	545:550	arg1	Presence					525:532	Presence	525:532	Presence of crystal phases of silica like quartz and cristoballite	525:590	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	4	49	theme	elemental	757:765	arg1	composition					767:777	The elemental composition	753:777	The elemental composition of the silica in ash	753:798	The elemental composition of the silica in ash is determined by EDX analysis.
25413790	1	50	theme	rice	306:309	arg1	ash					316:318	rice husk ash	306:318	rice husk ash by carbonization	306:335	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	4	51	theme	silica	786:791	arg1	composition					767:777	The elemental composition	753:777	The elemental composition of the silica in ash	753:798	The elemental composition of the silica in ash is determined by EDX analysis.
25413790	5	52	theme	transient	903:911	arg1	temperature					913:923	the transient temperature	899:923	the transient temperature of 840-850 °C	899:937	The exothermic reaction and the mass loss observed in the TG/DTG at the transient temperature of 840-850 °C has confirmed the presence of the α-quartz.
25413790	6	53	theme	various	1061:1067	arg1	shapes					1077:1082	the various crystal shapes	1057:1082	the various crystal shapes that were present in the sugarcane and husk ash	1057:1130	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	53	theme	various	1061:1067	arg1	present					1094:1100	present	1094:1100	present	1094:1100	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	3	54	theme	1100	718:721	arg1	-1					739:740	-1	739:740	-1	739:740	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	3	54	theme	1100	718:721	arg1	cm					736:737	1100, 820 and 620 cm	718:737	cm	736:737	Presence of crystal phases of silica like quartz and cristoballite is well indexed by the X-ray diffraction peaks that appeared at 2θ = 27, 40 and 60 which is further confirmed by the peaks at 1100, 820 and 620 cm(-1) of FT-IR.
25413790	1	55	from	presence	210:217	arg1	combustion					340:349	combustion	340:349	combustion	340:349	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	5	56	theme	mass	863:866	arg1	loss					868:871	the mass loss	859:871	the mass loss	859:871	The exothermic reaction and the mass loss observed in the TG/DTG at the transient temperature of 840-850 °C has confirmed the presence of the α-quartz.
25413790	2	57	from	Presence	403:410	arg1	samples					433:439	the ash samples	425:439	the ash samples	425:439	Presence of silica in the ash samples has been well characterized by the XRD, FT-IR, EDX, SEM and N2 sorption techniques.
25413790	1	58	theme	major	130:134	arg1	waste					142:146	the major solid waste	126:146	the major solid waste in the environment	126:165	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	1	58	theme	major	130:134	arg1	waste					114:118	Agricultural waste	101:118	Agricultural waste	101:118	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	6	59	located	present	1094:1100	arg1	husk					1123:1126	husk	1123:1126	husk	1123:1126	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	59	located	present	1094:1100	arg2	shapes					1077:1082	the various crystal shapes	1057:1082	the various crystal shapes that were present in the sugarcane and husk ash	1057:1130	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	59	located	present	1094:1100	arg1	sugarcane					1109:1117	sugarcane	1109:1117	sugarcane	1109:1117	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	59	located	present	1094:1100	arg2	present					1094:1100	present	1094:1100	present	1094:1100	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	5	60	from	temperature	913:923	arg1	TG/DTG					889:894	the TG/DTG	885:894	the TG/DTG at the transient temperature of 840-850 °C	885:937	The exothermic reaction and the mass loss observed in the TG/DTG at the transient temperature of 840-850 °C has confirmed the presence of the α-quartz.
25413790	1	61	dep	silica	242:247	arg1	ash					298:300	sugarcane bagasse ash	280:300	sugarcane bagasse ash	280:300	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	1	61	dep	silica	242:247	arg1	ash					316:318	rice husk ash	306:318	rice husk ash by carbonization	306:335	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	1	62	theme	solid	136:140	arg1	waste					142:146	the major solid waste	126:146	the major solid waste in the environment	126:165	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	1	62	theme	solid	136:140	arg1	waste					114:118	Agricultural waste	101:118	Agricultural waste	101:118	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	2	63	theme	XRD	476:478	arg1	techniques					513:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	the XRD, FT-IR, EDX, SEM and N2 sorption techniques	472:522	Presence of silica in the ash samples has been well characterized by the XRD, FT-IR, EDX, SEM and N2 sorption techniques.
25413790	1	64	theme	husk	311:314	arg1	ash					316:318	rice husk ash	306:318	rice husk ash by carbonization	306:335	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
25413790	0	65	dep	Carbonization	0:12	arg1	combustion					17:26	combustion	17:26	combustion	17:26	Carbonization on combustion and biodegradation of agricultural waste as a possible source of silica.
25413790	0	65	dep	Carbonization	0:12	arg1	biodegradation					32:45	biodegradation	32:45	biodegradation	32:45	Carbonization on combustion and biodegradation of agricultural waste as a possible source of silica.
25413790	6	66	from	husk	1123:1126	arg1	shapes					1077:1082	the various crystal shapes	1057:1082	the various crystal shapes that were present in the sugarcane and husk ash	1057:1130	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	6	66	from	husk	1123:1126	arg1	present					1094:1100	present	1094:1100	present	1094:1100	SEM micrograph has also supported the presence of silica and has revealed the various crystal shapes that were present in the sugarcane and husk ash.
25413790	1	67	theme	minerals	222:229	arg1	presence					210:217	the presence	206:217	the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion	206:349	Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation.
29103499	5	0	theme	hydrogel	1102:1109	arg1	groups					1111:1116	single hBMSCs- or BMP-2-immobilized hydrogel groups	1066:1116	single hBMSCs- or BMP-2-immobilized hydrogel groups	1066:1116	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	1	1	theme	/hyaluronic	133:143	arg1	HA					151:152	HA	151:152	HA	151:152	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	1	1	theme	/hyaluronic	133:143	arg1	acid					145:148	situ gelling alginate (ALG)/hyaluronic acid	106:148	situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent	106:281	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	5	2	theme	hBMSCs-	1073:1079	arg1	groups					1111:1116	single hBMSCs- or BMP-2-immobilized hydrogel groups	1066:1116	single hBMSCs- or BMP-2-immobilized hydrogel groups	1066:1116	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	0	3	theme	bone	79:82	arg1	graft					84:88	an injectable bone graft	65:88	an injectable bone graft	65:88	Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft.
29103499	0	3	theme	bone	79:82	arg1	hydrogel					53:60	in situ gelling polysaccharide hydrogel	22:60	in situ gelling polysaccharide hydrogel	22:60	Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft.
29103499	4	4	theme	HA	839:840	arg1	composition					842:852	HA composition	839:852	HA composition	839:852	Our findings from cell culture and animal study demonstrated that the osteogenic differentiation of hBMSCs was improved with increasing HA composition in the hydrogel.
29103499	4	5	from	animal	738:743	arg1	findings					707:714	Our findings	703:714	Our findings from cell culture and animal study	703:749	Our findings from cell culture and animal study demonstrated that the osteogenic differentiation of hBMSCs was improved with increasing HA composition in the hydrogel.
29103499	1	6	theme	acid	145:148	arg1	hydrogels					155:163	situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels	106:163	situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent	106:281	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	3	7	dep	in	511:512	arg1	vitro					514:518	vitro	514:518	vitro	514:518	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	1	8	theme	crosslinking	216:227	arg1	CaSO4					205:209	CaSO4	205:209	CaSO4	205:209	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	1	8	theme	crosslinking	216:227	arg1	agent					229:233	a crosslinking agent	214:233	a crosslinking agent	214:233	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	3	9	theme	mandibular	659:668	arg1	model					677:681	mandibular defect model	659:681	mandibular defect model of miniature pigs	659:699	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	4	10	theme	hBMSCs	803:808	arg1	differentiation					784:798	the osteogenic differentiation	769:798	the osteogenic differentiation of hBMSCs	769:808	Our findings from cell culture and animal study demonstrated that the osteogenic differentiation of hBMSCs was improved with increasing HA composition in the hydrogel.
29103499	3	11	theme	osteogenic	520:529	arg1	behavior					547:554	their in vitro osteogenic differentiation behavior	505:554	their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs)	505:595	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	5	12	theme	hBMSCs	971:976	arg1	differentiation					952:966	greatly enhanced osteogenic differentiation	924:966	greatly enhanced osteogenic differentiation of hBMSCs (in vitro)	924:987	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	5	12	theme	hBMSCs	971:976	arg1	regeneration					998:1009	bone regeneration	993:1009	bone regeneration (in vivo)	993:1019	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	5	13	theme	enhanced	932:939	arg1	differentiation					952:966	greatly enhanced osteogenic differentiation	924:966	greatly enhanced osteogenic differentiation of hBMSCs (in vitro)	924:987	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	3	14	theme	pigs	696:699	arg1	model					677:681	mandibular defect model	659:681	mandibular defect model of miniature pigs	659:699	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	4	15	theme	cell	721:724	arg1	culture					726:732	cell culture	721:732	cell culture	721:732	Our findings from cell culture and animal study demonstrated that the osteogenic differentiation of hBMSCs was improved with increasing HA composition in the hydrogel.
29103499	4	16	from	culture	726:732	arg1	findings					707:714	Our findings	703:714	Our findings from cell culture and animal study	703:749	Our findings from cell culture and animal study demonstrated that the osteogenic differentiation of hBMSCs was improved with increasing HA composition in the hydrogel.
29103499	3	17	theme	differentiation	531:545	arg1	behavior					547:554	their in vitro osteogenic differentiation behavior	505:554	their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs)	505:595	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	3	18	theme	different	459:467	arg1	ratios					476:481	different ALG/HA ratios	459:481	different ALG/HA ratios	459:481	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	1	19	theme	situ	106:109	arg1	HA					151:152	HA	151:152	HA	151:152	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	1	19	theme	situ	106:109	arg1	acid					145:148	situ gelling alginate (ALG)/hyaluronic acid	106:148	situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent	106:281	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	5	20	dep	regeneration	998:1009	arg1	vivo					1015:1018	in vivo	1012:1018	in vivo	1012:1018	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	3	21	theme	in	511:512	arg1	behavior					547:554	their in vitro osteogenic differentiation behavior	505:554	their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs)	505:595	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	5	22	theme	single	1066:1071	arg1	groups					1111:1116	single hBMSCs- or BMP-2-immobilized hydrogel groups	1066:1116	single hBMSCs- or BMP-2-immobilized hydrogel groups	1066:1116	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	3	23	dep	model	652:656	arg1	model					677:681	mandibular defect model	659:681	mandibular defect model of miniature pigs	659:699	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	5	24	theme	BMP-2-immobilized	1084:1100	arg1	groups					1111:1116	single hBMSCs- or BMP-2-immobilized hydrogel groups	1066:1116	single hBMSCs- or BMP-2-immobilized hydrogel groups	1066:1116	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	3	25	theme	ALG/HA	469:474	arg1	ratios					476:481	different ALG/HA ratios	459:481	different ALG/HA ratios	459:481	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	3	26	theme	miniature	686:694	arg1	pigs					696:699	miniature pigs	686:699	miniature pigs	686:699	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	3	27	theme	human	559:563	arg1	marrow					570:575	human bone marrow	559:575	human bone marrow stem cells (hBMSCs)	559:595	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	5	28	theme	ALG/HA	1039:1044	arg1	hydrogel					1046:1053	the ALG/HA hydrogel	1035:1053	the ALG/HA hydrogel itself	1035:1060	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	3	29	theme	bone	565:568	arg1	marrow					570:575	human bone marrow	559:575	human bone marrow stem cells (hBMSCs)	559:595	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	2	30	theme	protein-2	354:362	arg1	release					324:330	sustained release	314:330	sustained release of bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks	314:412	The ALG/HA hydrogels provided sustained release of bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks.
29103499	5	31	theme	ALG/HA	900:905	arg1	hydrogel					907:914	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel	871:914	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel	871:914	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	3	32	theme	ALG/HA	437:442	arg1	hydrogels					444:452	The BMP-2-immobilized ALG/HA hydrogels	415:452	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios	415:481	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	0	33	theme	in	22:23	arg1	graft					84:88	an injectable bone graft	65:88	an injectable bone graft	65:88	Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft.
29103499	0	33	theme	in	22:23	arg1	hydrogel					53:60	in situ gelling polysaccharide hydrogel	22:60	in situ gelling polysaccharide hydrogel	22:60	Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft.
29103499	5	34	theme	osteogenic	941:950	arg1	differentiation					952:966	greatly enhanced osteogenic differentiation	924:966	greatly enhanced osteogenic differentiation of hBMSCs (in vitro)	924:987	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	5	35	theme	hBMSCs/BMP-2-immobilized	875:898	arg1	hydrogel					907:914	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel	871:914	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel	871:914	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	1	36	theme	ALG	129:131	arg1	HA					151:152	HA	151:152	HA	151:152	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	1	36	theme	ALG	129:131	arg1	acid					145:148	situ gelling alginate (ALG)/hyaluronic acid	106:148	situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent	106:281	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	0	37	theme	polysaccharide	38:51	arg1	graft					84:88	an injectable bone graft	65:88	an injectable bone graft	65:88	Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft.
29103499	0	37	theme	polysaccharide	38:51	arg1	hydrogel					53:60	in situ gelling polysaccharide hydrogel	22:60	in situ gelling polysaccharide hydrogel	22:60	Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft.
29103499	1	38	theme	crosslinking	252:263	arg1	CaSO4					205:209	CaSO4	205:209	CaSO4	205:209	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	1	38	theme	crosslinking	252:263	arg1	agent					277:281	a crosslinking retardation agent	250:281	a crosslinking retardation agent	250:281	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	3	39	theme	cells	582:586	arg1	regeneration					614:625	in vivo bone regeneration	601:625	in vivo bone regeneration	601:625	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	3	39	theme	cells	582:586	arg1	behavior					547:554	their in vitro osteogenic differentiation behavior	505:554	their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs)	505:595	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	1	40	theme	controllable	172:183	arg1	rate					194:197	a controllable gelation rate	170:197	a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent	170:281	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	0	41	theme	gelling	30:36	arg1	graft					84:88	an injectable bone graft	65:88	an injectable bone graft	65:88	Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft.
29103499	0	41	theme	gelling	30:36	arg1	hydrogel					53:60	in situ gelling polysaccharide hydrogel	22:60	in situ gelling polysaccharide hydrogel	22:60	Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft.
29103499	1	42	theme	retardation	265:275	arg1	CaSO4					205:209	CaSO4	205:209	CaSO4	205:209	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	1	42	theme	retardation	265:275	arg1	agent					277:281	a crosslinking retardation agent	250:281	a crosslinking retardation agent	250:281	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	3	43	theme	in	601:602	arg1	regeneration					614:625	in vivo bone regeneration	601:625	in vivo bone regeneration	601:625	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	4	44	theme	osteogenic	773:782	arg1	differentiation					784:798	the osteogenic differentiation	769:798	the osteogenic differentiation of hBMSCs	769:808	Our findings from cell culture and animal study demonstrated that the osteogenic differentiation of hBMSCs was improved with increasing HA composition in the hydrogel.
29103499	1	45	theme	gelation	185:192	arg1	rate					194:197	a controllable gelation rate	170:197	a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent	170:281	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	3	46	theme	bone	609:612	arg1	regeneration					614:625	in vivo bone regeneration	601:625	in vivo bone regeneration	601:625	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	3	47	theme	marrow	570:575	arg1	cells					582:586	human bone marrow stem cells	559:586	human bone marrow stem cells (hBMSCs)	559:595	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	3	47	theme	marrow	570:575	arg1	hBMSCs					589:594	hBMSCs	589:594	hBMSCs	589:594	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	2	48	theme	sustained	314:322	arg1	release					324:330	sustained release	314:330	sustained release of bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks	314:412	The ALG/HA hydrogels provided sustained release of bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks.
29103499	3	49	with	hydrogels	444:452	arg1	ratios					476:481	different ALG/HA ratios	459:481	different ALG/HA ratios	459:481	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	1	50	with	hydrogels	155:163	arg1	rate					194:197	a controllable gelation rate	170:197	a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent	170:281	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	3	51	theme	animal	645:650	arg1	model					652:656	an animal model	642:656	an animal model (mandibular defect model of miniature pigs)	642:700	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	3	52	theme	stem	577:580	arg1	cells					582:586	human bone marrow stem cells	559:586	human bone marrow stem cells (hBMSCs)	559:595	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	3	52	theme	stem	577:580	arg1	hBMSCs					589:594	hBMSCs	589:594	hBMSCs	589:594	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	1	53	theme	gelling	111:117	arg1	HA					151:152	HA	151:152	HA	151:152	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	1	53	theme	gelling	111:117	arg1	acid					145:148	situ gelling alginate (ALG)/hyaluronic acid	106:148	situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent	106:281	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	1	54	theme	alginate	119:126	arg1	HA					151:152	HA	151:152	HA	151:152	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	1	54	theme	alginate	119:126	arg1	acid					145:148	situ gelling alginate (ALG)/hyaluronic acid	106:148	situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent	106:281	We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO4 as a crosslinking agent and Na2HPO4 as a crosslinking retardation agent.
29103499	3	55	dep	in	601:602	arg1	vivo					604:607	vivo	604:607	vivo	604:607	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	0	56	dep	in	22:23	arg1	situ					25:28	situ	25:28	situ	25:28	Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft.
29103499	2	57	theme	ALG/HA	288:293	arg1	hydrogels					295:303	The ALG/HA hydrogels	284:303	The ALG/HA hydrogels	284:303	The ALG/HA hydrogels provided sustained release of bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks.
29103499	5	58	dep	hBMSCs	971:976	arg1	vitro					982:986	in vitro	979:986	in vitro	979:986	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	0	59	theme	injectable	68:77	arg1	graft					84:88	an injectable bone graft	65:88	an injectable bone graft	65:88	Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft.
29103499	0	59	theme	injectable	68:77	arg1	hydrogel					53:60	in situ gelling polysaccharide hydrogel	22:60	in situ gelling polysaccharide hydrogel	22:60	Multivalent ion-based in situ gelling polysaccharide hydrogel as an injectable bone graft.
29103499	3	60	theme	BMP-2-immobilized	419:435	arg1	hydrogels					444:452	The BMP-2-immobilized ALG/HA hydrogels	415:452	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios	415:481	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	5	61	theme	bone	993:996	arg1	regeneration					998:1009	bone regeneration	993:1009	bone regeneration (in vivo)	993:1019	The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
29103499	3	62	dep	behavior	547:554	arg1	behavior					627:634	behavior	627:634	behavior	627:634	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	2	63	theme	morphogenetic	340:352	arg1	BMP-2					365:369	BMP-2	365:369	BMP-2	365:369	The ALG/HA hydrogels provided sustained release of bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks.
29103499	2	63	theme	morphogenetic	340:352	arg1	protein-2					354:362	bone morphogenetic protein-2	335:362	bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks	335:412	The ALG/HA hydrogels provided sustained release of bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks.
29103499	4	64	dep	culture	726:732	arg1	study					745:749	study	745:749	study	745:749	Our findings from cell culture and animal study demonstrated that the osteogenic differentiation of hBMSCs was improved with increasing HA composition in the hydrogel.
29103499	3	65	theme	defect	670:675	arg1	model					677:681	mandibular defect model	659:681	mandibular defect model of miniature pigs	659:699	The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs).
29103499	2	66	theme	bone	335:338	arg1	BMP-2					365:369	BMP-2	365:369	BMP-2	365:369	The ALG/HA hydrogels provided sustained release of bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks.
29103499	2	66	theme	bone	335:338	arg1	protein-2					354:362	bone morphogenetic protein-2	335:362	bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks	335:412	The ALG/HA hydrogels provided sustained release of bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks.
26751520	2	0	theme	cotton	391:396	arg1	fibers					398:403	natural cotton fibers	383:403	natural cotton fibers	383:403	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	2	1	theme	natural	383:389	arg1	fibers					398:403	natural cotton fibers	383:403	natural cotton fibers	383:403	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	4	2	theme	Silver	599:604	arg1	nanoparticles					606:618	Silver nanoparticles	599:618	Silver nanoparticles that were covalently attached to these sulfhydryl groups	599:675	Silver nanoparticles that were covalently attached to these sulfhydryl groups were assembled next.
26751520	1	3	theme	Antibacterial	39:51	arg1	cellulose					53:61	Antibacterial cellulose	39:61	Antibacterial cellulose	39:61	Antibacterial cellulose fibers through the covalent bonding of silver nanoparticles to electrospun fibers.
26751520	4	4	attach	attached	641:648	arg2	nanoparticles					606:618	Silver nanoparticles	599:618	Silver nanoparticles that were covalently attached to these sulfhydryl groups	599:675	Silver nanoparticles that were covalently attached to these sulfhydryl groups were assembled next.
26751520	4	4	attach	attached	641:648	arg1	groups					670:675	these sulfhydryl groups	653:675	these sulfhydryl groups	653:675	Silver nanoparticles that were covalently attached to these sulfhydryl groups were assembled next.
26751520	3	5	contain	containing	558:567	arg1	fibers					551:556	nano-cotton fibers	539:556	nano-cotton fibers containing reactive thiol functionality	539:596	The resulting nano-cotton fibers were esterified with trityl-3-mercaptopropionic acid, which after selective de-tritylation afforded nano-cotton fibers containing reactive thiol functionality.
26751520	3	5	contain	containing	558:567	arg2	functionality					584:596	reactive thiol functionality	569:596	reactive thiol functionality	569:596	The resulting nano-cotton fibers were esterified with trityl-3-mercaptopropionic acid, which after selective de-tritylation afforded nano-cotton fibers containing reactive thiol functionality.
26751520	5	6	theme	antibacterial	775:787	arg1	activity					789:796	the antibacterial activity	771:796	the antibacterial activity of the resulting nano-cotton Ag-nanoparticle composite	771:851	The microstructure of the resulting nanocomposite was characterized, and the antibacterial activity of the resulting nano-cotton Ag-nanoparticle composite was also studied.
26751520	2	7	theme	increased	348:356	arg1	area-relative					366:378	an increased surface area-relative	345:378	an increased surface area-relative to natural cotton fibers	345:403	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	1	8	theme	silver	102:107	arg1	nanoparticles					109:121	silver nanoparticles	102:121	silver nanoparticles	102:121	Antibacterial cellulose fibers through the covalent bonding of silver nanoparticles to electrospun fibers.
26751520	5	9	theme	resulting	724:732	arg1	nanocomposite					734:746	the resulting nanocomposite	720:746	the resulting nanocomposite	720:746	The microstructure of the resulting nanocomposite was characterized, and the antibacterial activity of the resulting nano-cotton Ag-nanoparticle composite was also studied.
26751520	6	10	theme	Gram-positive	949:961	arg1	bacteria					963:970	both Gram-negative and Gram-positive bacteria	926:970	both Gram-negative and Gram-positive bacteria	926:970	This nanocomposite showed significant activity against both Gram-negative and Gram-positive bacteria.
26751520	5	11	theme	nanocomposite	734:746	arg1	microstructure					702:715	The microstructure	698:715	The microstructure of the resulting nanocomposite	698:746	The microstructure of the resulting nanocomposite was characterized, and the antibacterial activity of the resulting nano-cotton Ag-nanoparticle composite was also studied.
26751520	3	12	theme	reactive	569:576	arg1	functionality					584:596	reactive thiol functionality	569:596	reactive thiol functionality	569:596	The resulting nano-cotton fibers were esterified with trityl-3-mercaptopropionic acid, which after selective de-tritylation afforded nano-cotton fibers containing reactive thiol functionality.
26751520	0	13	theme	natural	4:10	arg1	fibers					12:17	natural fibers	4:17	natural fibers	4:17	Can natural fibers be a silver bullet?
26751520	0	13	theme	natural	4:10	arg1	bullet					31:36	a silver bullet	22:36	a silver bullet	22:36	Can natural fibers be a silver bullet?
26751520	4	14	theme	sulfhydryl	659:668	arg1	groups					670:675	these sulfhydryl groups	653:675	these sulfhydryl groups	653:675	Silver nanoparticles that were covalently attached to these sulfhydryl groups were assembled next.
26751520	6	15	theme	Gram-negative	931:943	arg1	bacteria					963:970	both Gram-negative and Gram-positive bacteria	926:970	both Gram-negative and Gram-positive bacteria	926:970	This nanocomposite showed significant activity against both Gram-negative and Gram-positive bacteria.
26751520	2	16	theme	Natural	146:152	arg1	cotton					154:159	Natural cotton	146:159	Natural cotton	146:159	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	1	17	theme	covalent	82:89	arg1	bonding					91:97	the covalent bonding	78:97	the covalent bonding of silver nanoparticles to electrospun fibers	78:143	Antibacterial cellulose fibers through the covalent bonding of silver nanoparticles to electrospun fibers.
26751520	5	18	theme	nano-cotton	815:825	arg1	composite					843:851	the resulting nano-cotton Ag-nanoparticle composite	801:851	the resulting nano-cotton Ag-nanoparticle composite	801:851	The microstructure of the resulting nanocomposite was characterized, and the antibacterial activity of the resulting nano-cotton Ag-nanoparticle composite was also studied.
26751520	3	19	theme	resulting	410:418	arg1	fibers					432:437	The resulting nano-cotton fibers	406:437	The resulting nano-cotton fibers	406:437	The resulting nano-cotton fibers were esterified with trityl-3-mercaptopropionic acid, which after selective de-tritylation afforded nano-cotton fibers containing reactive thiol functionality.
26751520	5	20	theme	Ag-nanoparticle	827:841	arg1	composite					843:851	the resulting nano-cotton Ag-nanoparticle composite	801:851	the resulting nano-cotton Ag-nanoparticle composite	801:851	The microstructure of the resulting nanocomposite was characterized, and the antibacterial activity of the resulting nano-cotton Ag-nanoparticle composite was also studied.
26751520	2	21	theme	reduced	314:320	arg1	diameter-and					322:333	a substantially reduced diameter-and	298:333	a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers	298:403	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	3	22	theme	nano-cotton	539:549	arg1	fibers					551:556	nano-cotton fibers	539:556	nano-cotton fibers containing reactive thiol functionality	539:596	The resulting nano-cotton fibers were esterified with trityl-3-mercaptopropionic acid, which after selective de-tritylation afforded nano-cotton fibers containing reactive thiol functionality.
26751520	0	23	theme	silver	24:29	arg1	fibers					12:17	natural fibers	4:17	natural fibers	4:17	Can natural fibers be a silver bullet?
26751520	0	23	theme	silver	24:29	arg1	bullet					31:36	a silver bullet	22:36	a silver bullet	22:36	Can natural fibers be a silver bullet?
26751520	3	24	theme	thiol	578:582	arg1	functionality					584:596	reactive thiol functionality	569:596	reactive thiol functionality	569:596	The resulting nano-cotton fibers were esterified with trityl-3-mercaptopropionic acid, which after selective de-tritylation afforded nano-cotton fibers containing reactive thiol functionality.
26751520	5	25	theme	composite	843:851	arg1	activity					789:796	the antibacterial activity	771:796	the antibacterial activity of the resulting nano-cotton Ag-nanoparticle composite	771:851	The microstructure of the resulting nanocomposite was characterized, and the antibacterial activity of the resulting nano-cotton Ag-nanoparticle composite was also studied.
26751520	3	26	theme	selective	505:513	arg1	de-tritylation					515:528	selective de-tritylation	505:528	selective de-tritylation	505:528	The resulting nano-cotton fibers were esterified with trityl-3-mercaptopropionic acid, which after selective de-tritylation afforded nano-cotton fibers containing reactive thiol functionality.
26751520	3	27	theme	trityl-3-mercaptopropionic	460:485	arg1	acid					487:490	trityl-3-mercaptopropionic acid	460:490	trityl-3-mercaptopropionic acid	460:490	The resulting nano-cotton fibers were esterified with trityl-3-mercaptopropionic acid, which after selective de-tritylation afforded nano-cotton fibers containing reactive thiol functionality.
26751520	2	28	theme	wet-jet	239:245	arg1	electrospun					247:257	wet-jet electrospun	239:257	wet-jet electrospun	239:257	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	6	29	theme	significant	897:907	arg1	activity					909:916	significant activity	897:916	significant activity against both Gram-negative and Gram-positive bacteria	897:970	This nanocomposite showed significant activity against both Gram-negative and Gram-positive bacteria.
26751520	2	30	dep	diameter-and	322:333	arg1	area-relative					366:378	an increased surface area-relative	345:378	an increased surface area-relative to natural cotton fibers	345:403	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	1	31	theme	nanoparticles	109:121	arg1	bonding					91:97	the covalent bonding	78:97	the covalent bonding of silver nanoparticles to electrospun fibers	78:143	Antibacterial cellulose fibers through the covalent bonding of silver nanoparticles to electrospun fibers.
26751520	2	32	theme	liquid	203:208	arg1	1-ethyl-3-methyl					210:225	a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun	178:257	1-ethyl-3-methyl	210:225	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	2	33	theme	cotton	279:284	arg1	fibers					286:291	nanoscale cotton fibers	269:291	nanoscale cotton fibers	269:291	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	2	34	theme	surface	358:364	arg1	area-relative					366:378	an increased surface area-relative	345:378	an increased surface area-relative to natural cotton fibers	345:403	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	5	35	theme	resulting	805:813	arg1	composite					843:851	the resulting nano-cotton Ag-nanoparticle composite	801:851	the resulting nano-cotton Ag-nanoparticle composite	801:851	The microstructure of the resulting nanocomposite was characterized, and the antibacterial activity of the resulting nano-cotton Ag-nanoparticle composite was also studied.
26751520	2	36	theme	ionic	197:201	arg1	1-ethyl-3-methyl					210:225	a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun	178:257	1-ethyl-3-methyl	210:225	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	2	37	theme	nanoscale	269:277	arg1	fibers					286:291	nanoscale cotton fibers	269:291	nanoscale cotton fibers	269:291	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	1	38	theme	electrospun	126:136	arg1	fibers					138:143	electrospun fibers	126:143	electrospun fibers	126:143	Antibacterial cellulose fibers through the covalent bonding of silver nanoparticles to electrospun fibers.
26751520	2	39	theme	room-temperature	180:195	arg1	1-ethyl-3-methyl					210:225	a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun	178:257	1-ethyl-3-methyl	210:225	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	2	40	dep	1-ethyl-3-methyl	210:225	arg1	acetate					227:233	acetate	227:233	acetate	227:233	Natural cotton was dissolved in a room-temperature ionic liquid 1-ethyl-3-methyl acetate and wet-jet electrospun to obtain nanoscale cotton fibers with a substantially reduced diameter-and therefore an increased surface area-relative to natural cotton fibers.
26751520	3	41	theme	nano-cotton	420:430	arg1	fibers					432:437	The resulting nano-cotton fibers	406:437	The resulting nano-cotton fibers	406:437	The resulting nano-cotton fibers were esterified with trityl-3-mercaptopropionic acid, which after selective de-tritylation afforded nano-cotton fibers containing reactive thiol functionality.
26868545	2	0	theme	uronic	331:336	arg1	acid					338:341	uronic acid	331:341	uronic acid	331:341	The isolated pectin, subdivided into calcium bound and alkaline extractable pectin, was fully characterized in terms of uronic acid and other sugar composition, methylation and acetylation degree.
26868545	5	1	theme	waste	885:889	arg1	streams					891:897	food waste streams	880:897	food waste streams ever reported in the literature with a homogeneous methodology	880:960	Even if the data could not cover all the possible batch by batch and country variabilities, to date this represents the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology.
26868545	4	2	theme	biological	644:653	arg1	properties					655:664	different physicochemical and biological properties	614:664	different physicochemical and biological properties	614:664	These can have different physicochemical and biological properties, which are useful in a wide range of applications.
26868545	5	3	from	streams	891:897	arg1	characterization					858:873	the most complete pectin characterization	833:873	the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology	833:960	Even if the data could not cover all the possible batch by batch and country variabilities, to date this represents the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology.
26868545	4	4	theme	applications	703:714	arg1	range					694:698	a wide range	687:698	a wide range of applications	687:714	These can have different physicochemical and biological properties, which are useful in a wide range of applications.
26868545	4	5	theme	wide	689:692	arg1	range					694:698	a wide range	687:698	a wide range of applications	687:714	These can have different physicochemical and biological properties, which are useful in a wide range of applications.
26868545	0	6	from	waste	51:55	arg1	composition					19:29	composition	19:29	composition	19:29	Pectin content and composition from different food waste streams.
26868545	0	6	from	waste	51:55	arg1	content					7:13	Pectin content	0:13	Pectin content	0:13	Pectin content and composition from different food waste streams.
26868545	3	7	theme	pectin	495:500	arg1	structures					502:511	pectin structures	495:511	pectin structures	495:511	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	5	8	theme	pectin	851:856	arg1	characterization					858:873	the most complete pectin characterization	833:873	the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology	833:960	Even if the data could not cover all the possible batch by batch and country variabilities, to date this represents the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology.
26868545	2	9	theme	bound	256:260	arg1	pectin					287:292	calcium bound and alkaline extractable pectin	248:292	calcium bound and alkaline extractable pectin	248:292	The isolated pectin, subdivided into calcium bound and alkaline extractable pectin, was fully characterized in terms of uronic acid and other sugar composition, methylation and acetylation degree.
26868545	4	10	theme	different	614:622	arg1	properties					655:664	different physicochemical and biological properties	614:664	different physicochemical and biological properties	614:664	These can have different physicochemical and biological properties, which are useful in a wide range of applications.
26868545	1	11	theme	exploitation	143:154	arg1	potential					156:164	their exploitation potential	137:164	their exploitation potential	137:164	In the present paper, 26 food waste streams were selected according to their exploitation potential and investigated in terms of pectin content.
26868545	4	12	from	range	694:698	arg1	useful					677:682	useful	677:682	useful	677:682	These can have different physicochemical and biological properties, which are useful in a wide range of applications.
26868545	2	13	theme	composition	359:369	arg1	terms					322:326	terms	322:326	terms of uronic acid and other sugar composition, methylation and acetylation degree	322:405	The isolated pectin, subdivided into calcium bound and alkaline extractable pectin, was fully characterized in terms of uronic acid and other sugar composition, methylation and acetylation degree.
26868545	5	14	with	literature	920:929	arg1	methodology					950:960	a homogeneous methodology	936:960	a homogeneous methodology	936:960	Even if the data could not cover all the possible batch by batch and country variabilities, to date this represents the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology.
26868545	1	15	theme	present	73:79	arg1	paper					81:85	the present paper	69:85	the present paper	69:85	In the present paper, 26 food waste streams were selected according to their exploitation potential and investigated in terms of pectin content.
26868545	2	16	theme	sugar	353:357	arg1	composition					359:369	other sugar composition	347:369	other sugar composition	347:369	The isolated pectin, subdivided into calcium bound and alkaline extractable pectin, was fully characterized in terms of uronic acid and other sugar composition, methylation and acetylation degree.
26868545	0	17	theme	Pectin	0:5	arg1	content					7:13	Pectin content	0:13	Pectin content	0:13	Pectin content and composition from different food waste streams.
26868545	3	18	theme	huge	523:526	arg1	diversity					539:547	a huge structural diversity	521:547	a huge structural diversity	521:547	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	2	19	theme	other	347:351	arg1	composition					359:369	other sugar composition	347:369	other sugar composition	347:369	The isolated pectin, subdivided into calcium bound and alkaline extractable pectin, was fully characterized in terms of uronic acid and other sugar composition, methylation and acetylation degree.
26868545	5	20	theme	food	880:883	arg1	streams					891:897	food waste streams	880:897	food waste streams ever reported in the literature with a homogeneous methodology	880:960	Even if the data could not cover all the possible batch by batch and country variabilities, to date this represents the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology.
26868545	5	21	theme	homogeneous	938:948	arg1	methodology					950:960	a homogeneous methodology	936:960	a homogeneous methodology	936:960	Even if the data could not cover all the possible batch by batch and country variabilities, to date this represents the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology.
26868545	3	22	theme	waste	431:435	arg1	source					463:468	a valuable source	452:468	a valuable source of pectin	452:478	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	3	22	theme	waste	431:435	arg1	streams					437:443	many waste streams	426:443	many waste streams	426:443	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	3	23	theme	structures	587:596	arg1	range					571:575	a broad range	563:575	a broad range of pectin structures	563:596	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	2	24	theme	extractable	275:285	arg1	pectin					287:292	calcium bound and alkaline extractable pectin	248:292	calcium bound and alkaline extractable pectin	248:292	The isolated pectin, subdivided into calcium bound and alkaline extractable pectin, was fully characterized in terms of uronic acid and other sugar composition, methylation and acetylation degree.
26868545	0	25	theme	different	36:44	arg1	waste					51:55	different food waste	36:55	different food waste	36:55	Pectin content and composition from different food waste streams.
26868545	3	26	theme	valuable	454:461	arg1	source					463:468	a valuable source	452:468	a valuable source of pectin	452:478	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	3	26	theme	valuable	454:461	arg1	streams					437:443	many waste streams	426:443	many waste streams	426:443	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	2	27	theme	isolated	215:222	arg1	pectin					224:229	The isolated pectin	211:229	The isolated pectin	211:229	The isolated pectin, subdivided into calcium bound and alkaline extractable pectin, was fully characterized in terms of uronic acid and other sugar composition, methylation and acetylation degree.
26868545	1	28	theme	pectin	195:200	arg1	content					202:208	pectin content	195:208	pectin content	195:208	In the present paper, 26 food waste streams were selected according to their exploitation potential and investigated in terms of pectin content.
26868545	3	29	theme	pectin	580:585	arg1	structures					587:596	pectin structures	580:596	pectin structures	580:596	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	3	30	theme	structural	528:537	arg1	diversity					539:547	a huge structural diversity	521:547	a huge structural diversity	521:547	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	1	31	theme	food	91:94	arg1	streams					102:108	26 food waste streams	88:108	26 food waste streams	88:108	In the present paper, 26 food waste streams were selected according to their exploitation potential and investigated in terms of pectin content.
26868545	0	32	theme	food	46:49	arg1	waste					51:55	different food waste	36:55	different food waste	36:55	Pectin content and composition from different food waste streams.
26868545	4	33	contain	have	609:612	arg2	properties					655:664	different physicochemical and biological properties	614:664	different physicochemical and biological properties	614:664	These can have different physicochemical and biological properties, which are useful in a wide range of applications.
26868545	4	33	contain	have	609:612	arg1	These					599:603	These	599:603	These	599:603	These can have different physicochemical and biological properties, which are useful in a wide range of applications.
26868545	1	34	theme	waste	96:100	arg1	streams					102:108	26 food waste streams	88:108	26 food waste streams	88:108	In the present paper, 26 food waste streams were selected according to their exploitation potential and investigated in terms of pectin content.
26868545	4	35	from	useful	677:682	arg1	range					694:698	a wide range	687:698	a wide range of applications	687:714	These can have different physicochemical and biological properties, which are useful in a wide range of applications.
26868545	3	36	theme	broad	565:569	arg1	range					571:575	a broad range	563:575	a broad range of pectin structures	563:596	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	5	37	theme	complete	842:849	arg1	characterization					858:873	the most complete pectin characterization	833:873	the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology	833:960	Even if the data could not cover all the possible batch by batch and country variabilities, to date this represents the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology.
26868545	1	38	theme	content	202:208	arg1	terms					186:190	terms	186:190	terms of pectin content	186:208	In the present paper, 26 food waste streams were selected according to their exploitation potential and investigated in terms of pectin content.
26868545	5	39	dep	batch	776:780	arg1	variabilities					794:806	variabilities	794:806	variabilities	794:806	Even if the data could not cover all the possible batch by batch and country variabilities, to date this represents the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology.
26868545	4	40	theme	physicochemical	624:638	arg1	properties					655:664	different physicochemical and biological properties	614:664	different physicochemical and biological properties	614:664	These can have different physicochemical and biological properties, which are useful in a wide range of applications.
26868545	3	41	theme	many	426:429	arg1	source					463:468	a valuable source	452:468	a valuable source of pectin	452:478	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	3	41	theme	many	426:429	arg1	streams					437:443	many waste streams	426:443	many waste streams	426:443	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	5	42	theme	possible	758:765	arg1	batch					767:771	all the possible batch	750:771	all the possible batch by batch and country variabilities	750:806	Even if the data could not cover all the possible batch by batch and country variabilities, to date this represents the most complete pectin characterization from food waste streams ever reported in the literature with a homogeneous methodology.
26868545	3	43	theme	pectin	473:478	arg1	source					463:468	a valuable source	452:468	a valuable source of pectin	452:478	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	3	43	theme	pectin	473:478	arg1	streams					437:443	many waste streams	426:443	many waste streams	426:443	It was shown that many waste streams can be a valuable source of pectin, but also that pectin structures present a huge structural diversity, resulting in a broad range of pectin structures.
26868545	2	44	dep	composition	359:369	arg1	degree					400:405	degree	400:405	degree	400:405	The isolated pectin, subdivided into calcium bound and alkaline extractable pectin, was fully characterized in terms of uronic acid and other sugar composition, methylation and acetylation degree.
26868545	2	45	theme	acid	338:341	arg1	terms					322:326	terms	322:326	terms of uronic acid and other sugar composition, methylation and acetylation degree	322:405	The isolated pectin, subdivided into calcium bound and alkaline extractable pectin, was fully characterized in terms of uronic acid and other sugar composition, methylation and acetylation degree.
25723888	0	0	theme	enhanced	78:85	arg1	adsorption					94:103	enhanced Pb(2+) adsorption	78:103	enhanced Pb(2+) adsorption from water	78:114	In situ growing directional spindle TiO2 nanocrystals on cellulose fibers for enhanced Pb(2+) adsorption from water.
25723888	4	1	theme	better	629:634	arg1	use					636:638	better use	629:638	better use of their surface area to adsorb heavy metals	629:683	These crystals were further controlled to disperse uniformly without agglomeration for better use of their surface area to adsorb heavy metals.
25723888	7	2	theme	pure	1037:1040	arg1	bed					1045:1047	pure CF bed	1037:1047	pure CF bed	1037:1047	A 12-fold increase in filtered bed volume was achieved for TiO2/CF bed compared with pure CF bed before breakthrough took place.
25723888	1	3	theme	cellulose	227:235	arg1	fibers					237:242	cellulose fibers	227:242	cellulose fibers (CF)	227:247	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	1	3	theme	cellulose	227:235	arg1	CF					245:246	CF	245:246	CF	245:246	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	6	4	theme	composite	825:833	arg1	fibers					835:840	the composite fibers	821:840	the composite fibers	821:840	In addition, the composite fibers were readily fabricated into a nonwoven filter bed through which dynamic filtration experiment was conducted.
25723888	7	5	theme	CF	1042:1043	arg1	bed					1045:1047	pure CF bed	1037:1047	pure CF bed	1037:1047	A 12-fold increase in filtered bed volume was achieved for TiO2/CF bed compared with pure CF bed before breakthrough took place.
25723888	6	6	theme	dynamic	907:913	arg1	experiment					926:935	dynamic filtration experiment	907:935	dynamic filtration experiment	907:935	In addition, the composite fibers were readily fabricated into a nonwoven filter bed through which dynamic filtration experiment was conducted.
25723888	3	7	theme	rutile	473:478	arg1	crystals					485:492	spindle rutile TiO2 crystals	465:492	spindle rutile TiO2 crystals	465:492	As a result, spindle rutile TiO2 crystals were nicely formed on the surface of cellulose.
25723888	3	7	theme	rutile	473:478	arg1	result					457:462	a result	455:462	a result	455:462	As a result, spindle rutile TiO2 crystals were nicely formed on the surface of cellulose.
25723888	0	8	theme	Pb	87:88	arg1	adsorption					94:103	enhanced Pb(2+) adsorption	78:103	enhanced Pb(2+) adsorption from water	78:114	In situ growing directional spindle TiO2 nanocrystals on cellulose fibers for enhanced Pb(2+) adsorption from water.
25723888	3	9	theme	TiO2	480:483	arg1	crystals					485:492	spindle rutile TiO2 crystals	465:492	spindle rutile TiO2 crystals	465:492	As a result, spindle rutile TiO2 crystals were nicely formed on the surface of cellulose.
25723888	3	9	theme	TiO2	480:483	arg1	result					457:462	a result	455:462	a result	455:462	As a result, spindle rutile TiO2 crystals were nicely formed on the surface of cellulose.
25723888	8	10	theme	water	1212:1216	arg1	decontamination					1218:1232	water decontamination	1212:1232	water decontamination	1212:1232	This work provides a green pathway for fabricating low cost, high efficiency and engineering application possible nanosorbents for water decontamination.
25723888	7	11	theme	TiO2/CF	1011:1017	arg1	bed					1019:1021	TiO2/CF bed	1011:1021	TiO2/CF bed compared with pure CF bed before breakthrough took place	1011:1078	A 12-fold increase in filtered bed volume was achieved for TiO2/CF bed compared with pure CF bed before breakthrough took place.
25723888	5	12	theme	lead	785:788	arg1	removal					799:805	lead (Pb(2+)) removal	785:805	lead (Pb(2+)) removal	785:805	The TiO2/CF composite showed enhanced adsorption capacity, good regenerability and selectivity for lead (Pb(2+)) removal.
25723888	5	13	theme	adsorption	724:733	arg1	capacity					735:742	enhanced adsorption capacity	715:742	enhanced adsorption capacity	715:742	The TiO2/CF composite showed enhanced adsorption capacity, good regenerability and selectivity for lead (Pb(2+)) removal.
25723888	8	14	theme	low	1132:1134	arg1	cost					1136:1139	low cost	1132:1139	low cost	1132:1139	This work provides a green pathway for fabricating low cost, high efficiency and engineering application possible nanosorbents for water decontamination.
25723888	5	15	theme	enhanced	715:722	arg1	capacity					735:742	enhanced adsorption capacity	715:742	enhanced adsorption capacity	715:742	The TiO2/CF composite showed enhanced adsorption capacity, good regenerability and selectivity for lead (Pb(2+)) removal.
25723888	3	16	theme	spindle	465:471	arg1	crystals					485:492	spindle rutile TiO2 crystals	465:492	spindle rutile TiO2 crystals	465:492	As a result, spindle rutile TiO2 crystals were nicely formed on the surface of cellulose.
25723888	3	16	theme	spindle	465:471	arg1	result					457:462	a result	455:462	a result	455:462	As a result, spindle rutile TiO2 crystals were nicely formed on the surface of cellulose.
25723888	1	17	theme	in	165:166	arg1	generation					173:182	in situ generation	165:182	in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF)	165:247	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	5	18	theme	good	745:748	arg1	regenerability					750:763	good regenerability	745:763	good regenerability	745:763	The TiO2/CF composite showed enhanced adsorption capacity, good regenerability and selectivity for lead (Pb(2+)) removal.
25723888	4	19	theme	heavy	672:676	arg1	metals					678:683	heavy metals	672:683	heavy metals	672:683	These crystals were further controlled to disperse uniformly without agglomeration for better use of their surface area to adsorb heavy metals.
25723888	6	20	theme	nonwoven	873:880	arg1	bed					889:891	a nonwoven filter bed	871:891	a nonwoven filter bed through which dynamic filtration experiment was conducted	871:949	In addition, the composite fibers were readily fabricated into a nonwoven filter bed through which dynamic filtration experiment was conducted.
25723888	8	21	theme	high	1142:1145	arg1	efficiency					1147:1156	high efficiency	1142:1156	high efficiency	1142:1156	This work provides a green pathway for fabricating low cost, high efficiency and engineering application possible nanosorbents for water decontamination.
25723888	6	22	theme	filtration	915:924	arg1	experiment					926:935	dynamic filtration experiment	907:935	dynamic filtration experiment	907:935	In addition, the composite fibers were readily fabricated into a nonwoven filter bed through which dynamic filtration experiment was conducted.
25723888	8	23	theme	efficiency	1147:1156	arg1	nanosorbents					1195:1206	low cost, high efficiency and engineering application possible nanosorbents	1132:1206	low cost, high efficiency and engineering application possible nanosorbents for water decontamination	1132:1232	This work provides a green pathway for fabricating low cost, high efficiency and engineering application possible nanosorbents for water decontamination.
25723888	0	24	theme	directional	16:26	arg1	nanocrystals					41:52	directional spindle TiO2 nanocrystals	16:52	directional spindle TiO2 nanocrystals	16:52	In situ growing directional spindle TiO2 nanocrystals on cellulose fibers for enhanced Pb(2+) adsorption from water.
25723888	6	25	theme	filter	882:887	arg1	bed					889:891	a nonwoven filter bed	871:891	a nonwoven filter bed through which dynamic filtration experiment was conducted	871:949	In addition, the composite fibers were readily fabricated into a nonwoven filter bed through which dynamic filtration experiment was conducted.
25723888	8	26	theme	application	1174:1184	arg1	nanosorbents					1195:1206	low cost, high efficiency and engineering application possible nanosorbents	1132:1206	low cost, high efficiency and engineering application possible nanosorbents for water decontamination	1132:1232	This work provides a green pathway for fabricating low cost, high efficiency and engineering application possible nanosorbents for water decontamination.
25723888	8	27	theme	green	1102:1106	arg1	pathway					1108:1114	a green pathway	1100:1114	a green pathway for fabricating low cost, high efficiency and engineering application possible nanosorbents for water decontamination	1100:1232	This work provides a green pathway for fabricating low cost, high efficiency and engineering application possible nanosorbents for water decontamination.
25723888	1	28	theme	facile	253:258	arg1	hydrolysis					260:269	facile hydrolysis	253:269	facile hydrolysis of TiOSO4	253:279	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	2	29	theme	TiO2	333:336	arg1	NPs					353:355	NPs	353:355	NPs	353:355	Cellulose was intended as a scaffold to immobilize TiO2 nanoparticles (NPs), but turned out surprisingly to be also a chemical template that directed the crystal growth.
25723888	2	29	theme	TiO2	333:336	arg1	nanoparticles					338:350	TiO2 nanoparticles	333:350	TiO2 nanoparticles (NPs)	333:356	Cellulose was intended as a scaffold to immobilize TiO2 nanoparticles (NPs), but turned out surprisingly to be also a chemical template that directed the crystal growth.
25723888	1	30	theme	titanium	187:194	arg1	TiO2					205:208	TiO2	205:208	TiO2	205:208	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	1	30	theme	titanium	187:194	arg1	dioxide					196:202	titanium dioxide	187:202	titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF)	187:247	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	0	31	theme	TiO2	36:39	arg1	nanocrystals					41:52	directional spindle TiO2 nanocrystals	16:52	directional spindle TiO2 nanocrystals	16:52	In situ growing directional spindle TiO2 nanocrystals on cellulose fibers for enhanced Pb(2+) adsorption from water.
25723888	7	32	theme	filtered	974:981	arg1	volume					987:992	filtered bed volume	974:992	filtered bed volume	974:992	A 12-fold increase in filtered bed volume was achieved for TiO2/CF bed compared with pure CF bed before breakthrough took place.
25723888	8	33	theme	engineering	1162:1172	arg1	application					1174:1184	engineering application	1162:1184	engineering application	1162:1184	This work provides a green pathway for fabricating low cost, high efficiency and engineering application possible nanosorbents for water decontamination.
25723888	1	34	theme	dioxide	196:202	arg1	nanocrystals					211:222	titanium dioxide (TiO2) nanocrystals	187:222	titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF)	187:247	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	0	35	theme	spindle	28:34	arg1	nanocrystals					41:52	directional spindle TiO2 nanocrystals	16:52	directional spindle TiO2 nanocrystals	16:52	In situ growing directional spindle TiO2 nanocrystals on cellulose fibers for enhanced Pb(2+) adsorption from water.
25723888	1	36	from	fibers	237:242	arg1	generation					173:182	in situ generation	165:182	in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF)	165:247	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	7	37	from	increase	962:969	arg1	volume					987:992	filtered bed volume	974:992	filtered bed volume	974:992	A 12-fold increase in filtered bed volume was achieved for TiO2/CF bed compared with pure CF bed before breakthrough took place.
25723888	8	38	theme	possible	1186:1193	arg1	nanosorbents					1195:1206	low cost, high efficiency and engineering application possible nanosorbents	1132:1206	low cost, high efficiency and engineering application possible nanosorbents for water decontamination	1132:1232	This work provides a green pathway for fabricating low cost, high efficiency and engineering application possible nanosorbents for water decontamination.
25723888	2	39	theme	chemical	400:407	arg1	template					409:416	a chemical template	398:416	a chemical template that directed the crystal growth	398:449	Cellulose was intended as a scaffold to immobilize TiO2 nanoparticles (NPs), but turned out surprisingly to be also a chemical template that directed the crystal growth.
25723888	2	39	theme	chemical	400:407	arg1	Cellulose					282:290	Cellulose	282:290	Cellulose	282:290	Cellulose was intended as a scaffold to immobilize TiO2 nanoparticles (NPs), but turned out surprisingly to be also a chemical template that directed the crystal growth.
25723888	1	40	theme	TiOSO4	274:279	arg1	hydrolysis					260:269	facile hydrolysis	253:269	facile hydrolysis of TiOSO4	253:279	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	1	41	from	nanocrystals	211:222	arg1	fibers					237:242	cellulose fibers	227:242	cellulose fibers (CF)	227:247	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	1	41	from	nanocrystals	211:222	arg1	CF					245:246	CF	245:246	CF	245:246	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	2	42	theme	crystal	436:442	arg1	growth					444:449	the crystal growth	432:449	the crystal growth	432:449	Cellulose was intended as a scaffold to immobilize TiO2 nanoparticles (NPs), but turned out surprisingly to be also a chemical template that directed the crystal growth.
25723888	4	43	theme	surface	649:655	arg1	area					657:660	their surface area	643:660	their surface area	643:660	These crystals were further controlled to disperse uniformly without agglomeration for better use of their surface area to adsorb heavy metals.
25723888	7	44	theme	bed	983:985	arg1	volume					987:992	filtered bed volume	974:992	filtered bed volume	974:992	A 12-fold increase in filtered bed volume was achieved for TiO2/CF bed compared with pure CF bed before breakthrough took place.
25723888	1	45	dep	in	165:166	arg1	situ					168:171	situ	168:171	situ	168:171	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	5	46	theme	TiO2/CF	690:696	arg1	composite					698:706	The TiO2/CF composite	686:706	The TiO2/CF composite	686:706	The TiO2/CF composite showed enhanced adsorption capacity, good regenerability and selectivity for lead (Pb(2+)) removal.
25723888	7	47	theme	12-fold	954:960	arg1	increase					962:969	A 12-fold increase	952:969	A 12-fold increase in filtered bed volume	952:992	A 12-fold increase in filtered bed volume was achieved for TiO2/CF bed compared with pure CF bed before breakthrough took place.
25723888	4	48	theme	area	657:660	arg1	use					636:638	better use	629:638	better use of their surface area to adsorb heavy metals	629:683	These crystals were further controlled to disperse uniformly without agglomeration for better use of their surface area to adsorb heavy metals.
25723888	8	49	theme	cost	1136:1139	arg1	nanosorbents					1195:1206	low cost, high efficiency and engineering application possible nanosorbents	1132:1206	low cost, high efficiency and engineering application possible nanosorbents for water decontamination	1132:1232	This work provides a green pathway for fabricating low cost, high efficiency and engineering application possible nanosorbents for water decontamination.
25723888	0	50	from	water	110:114	arg1	adsorption					94:103	enhanced Pb(2+) adsorption	78:103	enhanced Pb(2+) adsorption from water	78:114	In situ growing directional spindle TiO2 nanocrystals on cellulose fibers for enhanced Pb(2+) adsorption from water.
25723888	3	51	theme	cellulose	531:539	arg1	surface					520:526	the surface	516:526	the surface of cellulose	516:539	As a result, spindle rutile TiO2 crystals were nicely formed on the surface of cellulose.
25723888	1	52	from	generation	173:182	arg1	fibers					237:242	cellulose fibers	227:242	cellulose fibers (CF)	227:247	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	1	52	from	generation	173:182	arg1	CF					245:246	CF	245:246	CF	245:246	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	1	53	theme	TiO2/cellulose	117:130	arg1	nanocomposite					132:144	TiO2/cellulose nanocomposite	117:144	TiO2/cellulose nanocomposite	117:144	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	1	54	theme	nanocrystals	211:222	arg1	generation					173:182	in situ generation	165:182	in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF)	165:247	TiO2/cellulose nanocomposite was synthesized by in situ generation of titanium dioxide (TiO2) nanocrystals on cellulose fibers (CF) via facile hydrolysis of TiOSO4.
25723888	0	55	theme	cellulose	57:65	arg1	fibers					67:72	cellulose fibers	57:72	cellulose fibers for enhanced Pb(2+) adsorption from water	57:114	In situ growing directional spindle TiO2 nanocrystals on cellulose fibers for enhanced Pb(2+) adsorption from water.
25723888	5	56	dep	lead	785:788	arg1	2+					794:795	2+	794:795	2+	794:795	The TiO2/CF composite showed enhanced adsorption capacity, good regenerability and selectivity for lead (Pb(2+)) removal.
25723888	5	56	dep	lead	785:788	arg1	Pb					791:792	Pb	791:792	Pb(2+)	791:796	The TiO2/CF composite showed enhanced adsorption capacity, good regenerability and selectivity for lead (Pb(2+)) removal.
28723346	2	0	theme	BET	451:453	arg1	area					463:466	a BET surface area	449:466	a BET surface area of 26.50m2/g	449:479	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	5	1	theme	prepared	822:829	arg1	catalyst					854:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst	818:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst	818:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	5	1	theme	prepared	822:829	arg1	active					873:878	active	873:878	active	873:878	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	5	2	theme	@	836:836	arg1	catalyst					854:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst	818:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst	818:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	5	2	theme	@	836:836	arg1	active					873:878	active	873:878	active	873:878	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	0	3	theme	efficient	78:86	arg1	catalysts					100:108	efficient, recyclable catalysts	78:108	efficient, recyclable catalysts used in ullmann reaction	78:133	Co-immobilization of Pd and Zn nanoparticles in chitosan/silica membranes for efficient, recyclable catalysts used in ullmann reaction.
28723346	5	4	theme	CS/SiO2	837:843	arg1	catalyst					854:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst	818:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst	818:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	5	4	theme	CS/SiO2	837:843	arg1	active					873:878	active	873:878	active	873:878	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	6	5	theme	same	1139:1142	arg1	material					1152:1159	the same carrier material	1135:1159	the same carrier material	1135:1159	This work supplies a successful approach to realize Pd catalysts and Zn reducing reagent co-immobilized in the same carrier material with excellent catalytic performances.
28723346	6	6	theme	Zn	1097:1098	arg1	reagent					1109:1115	Zn reducing reagent	1097:1115	Zn reducing reagent	1097:1115	This work supplies a successful approach to realize Pd catalysts and Zn reducing reagent co-immobilized in the same carrier material with excellent catalytic performances.
28723346	5	7	theme	Pd-Zn	831:835	arg1	catalyst					854:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst	818:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst	818:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	5	7	theme	Pd-Zn	831:835	arg1	active					873:878	active	873:878	active	873:878	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	1	8	theme	SiO2	340:343	arg1	technique					345:353	SiO2 technique	340:353	SiO2 technique	340:353	In this study, an efficient heterogeneous catalyst including palladium (Pd) and zinc (Zn) nanoparticles supported on chitosan/silica (CS/SiO2) composite membrane is synthesized using partially etching of SiO2 technique.
28723346	2	9	theme	26.50m2/g	471:479	arg1	area					463:466	a BET surface area	449:466	a BET surface area of 26.50m2/g	449:479	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	1	10	theme	technique	345:353	arg1	etching					329:335	partially etching	319:335	partially etching of SiO2 technique	319:353	In this study, an efficient heterogeneous catalyst including palladium (Pd) and zinc (Zn) nanoparticles supported on chitosan/silica (CS/SiO2) composite membrane is synthesized using partially etching of SiO2 technique.
28723346	3	11	theme	transmission	498:509	arg1	microscopy					520:529	High-resolution transmission electron microscopy	482:529	High-resolution transmission electron microscopy (HR-TEM)	482:538	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	3	11	theme	transmission	498:509	arg1	HR-TEM					532:537	HR-TEM	532:537	HR-TEM	532:537	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	1	12	theme	efficient	154:162	arg1	catalyst					178:185	an efficient heterogeneous catalyst	151:185	an efficient heterogeneous catalyst including palladium (Pd) and zinc (Zn)	151:224	In this study, an efficient heterogeneous catalyst including palladium (Pd) and zinc (Zn) nanoparticles supported on chitosan/silica (CS/SiO2) composite membrane is synthesized using partially etching of SiO2 technique.
28723346	4	13	theme	X-ray	770:774	arg1	diffraction					776:786	X-ray diffraction study and HR-TEM observation	770:815	diffraction	776:786	Zinc crystal was also detected by X-ray diffraction study and HR-TEM observation.
28723346	3	14	theme	electron	511:518	arg1	microscopy					520:529	High-resolution transmission electron microscopy	482:529	High-resolution transmission electron microscopy (HR-TEM)	482:538	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	3	14	theme	electron	511:518	arg1	HR-TEM					532:537	HR-TEM	532:537	HR-TEM	532:537	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	1	15	theme	heterogeneous	164:176	arg1	catalyst					178:185	an efficient heterogeneous catalyst	151:185	an efficient heterogeneous catalyst including palladium (Pd) and zinc (Zn)	151:224	In this study, an efficient heterogeneous catalyst including palladium (Pd) and zinc (Zn) nanoparticles supported on chitosan/silica (CS/SiO2) composite membrane is synthesized using partially etching of SiO2 technique.
28723346	3	16	theme	Zn0	667:669	arg1	aggregates					671:680	Zn0 aggregates	667:680	Zn0 aggregates (about 10-15nm)	667:696	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	2	17	theme	isotherm	368:375	arg1	results					377:383	N2 sorption isotherm results	356:383	N2 sorption isotherm results	356:383	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	1	18	theme	chitosan/silica	253:267	arg1	membrane					289:296	chitosan/silica (CS/SiO2) composite membrane	253:296	chitosan/silica (CS/SiO2) composite membrane	253:296	In this study, an efficient heterogeneous catalyst including palladium (Pd) and zinc (Zn) nanoparticles supported on chitosan/silica (CS/SiO2) composite membrane is synthesized using partially etching of SiO2 technique.
28723346	3	19	theme	Pd0	632:634	arg1	nanoparticles					636:648	the Pd0 nanoparticles	628:648	the Pd0 nanoparticles (below 5nm)	628:660	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	0	20	dep	efficient	78:86	arg1	recyclable					89:98	recyclable	89:98	recyclable	89:98	Co-immobilization of Pd and Zn nanoparticles in chitosan/silica membranes for efficient, recyclable catalysts used in ullmann reaction.
28723346	2	21	theme	sorption	359:366	arg1	results					377:383	N2 sorption isotherm results	356:383	N2 sorption isotherm results	356:383	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	2	22	theme	CS/SiO2	415:421	arg1	membrane					436:443	the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane	396:443	the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane	396:443	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	5	23	theme	activities	1016:1025	arg1	loss					1008:1011	significant loss	996:1011	significant loss of activities	996:1025	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	2	24	contain	had	445:447	arg2	area					463:466	a BET surface area	449:466	a BET surface area of 26.50m2/g	449:479	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	2	24	contain	had	445:447	arg1	membrane					436:443	the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane	396:443	the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane	396:443	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	5	25	theme	Ullmann	884:890	arg1	reactions					915:923	Ullmann reductive homocoupling reactions	884:923	Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides	884:957	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	2	26	theme	Pd-Zn	409:413	arg1	1/1					424:426	1/1	424:426	1/1	424:426	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	2	26	theme	Pd-Zn	409:413	arg1	CS/SiO2					415:421	Pd-Zn@CS/SiO2	409:421	the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane	396:443	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	2	27	theme	@	414:414	arg1	1/1					424:426	1/1	424:426	1/1	424:426	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	2	27	theme	@	414:414	arg1	CS/SiO2					415:421	Pd-Zn@CS/SiO2	409:421	the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane	396:443	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	1	28	theme	CS/SiO2	270:276	arg1	membrane					289:296	chitosan/silica (CS/SiO2) composite membrane	253:296	chitosan/silica (CS/SiO2) composite membrane	253:296	In this study, an efficient heterogeneous catalyst including palladium (Pd) and zinc (Zn) nanoparticles supported on chitosan/silica (CS/SiO2) composite membrane is synthesized using partially etching of SiO2 technique.
28723346	0	29	theme	ullmann	118:124	arg1	reaction					126:133	ullmann reaction	118:133	ullmann reaction	118:133	Co-immobilization of Pd and Zn nanoparticles in chitosan/silica membranes for efficient, recyclable catalysts used in ullmann reaction.
28723346	5	30	theme	significant	996:1006	arg1	loss					1008:1011	significant loss	996:1011	significant loss of activities	996:1025	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	5	31	theme	reductive	892:900	arg1	reactions					915:923	Ullmann reductive homocoupling reactions	884:923	Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides	884:957	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	2	32	theme	porous	429:434	arg1	membrane					436:443	the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane	396:443	the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane	396:443	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	2	33	theme	prepared	400:407	arg1	membrane					436:443	the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane	396:443	the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane	396:443	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	5	34	theme	bromides	950:957	arg1	reactions					915:923	Ullmann reductive homocoupling reactions	884:923	Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides	884:957	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	6	35	theme	catalytic	1176:1184	arg1	performances					1186:1197	excellent catalytic performances	1166:1197	excellent catalytic performances	1166:1197	This work supplies a successful approach to realize Pd catalysts and Zn reducing reagent co-immobilized in the same carrier material with excellent catalytic performances.
28723346	0	36	theme	Zn	28:29	arg1	Co-immobilization					0:16	Co-immobilization	0:16	Co-immobilization of Pd and Zn	0:29	Co-immobilization of Pd and Zn nanoparticles in chitosan/silica membranes for efficient, recyclable catalysts used in ullmann reaction.
28723346	1	37	theme	composite	279:287	arg1	membrane					289:296	chitosan/silica (CS/SiO2) composite membrane	253:296	chitosan/silica (CS/SiO2) composite membrane	253:296	In this study, an efficient heterogeneous catalyst including palladium (Pd) and zinc (Zn) nanoparticles supported on chitosan/silica (CS/SiO2) composite membrane is synthesized using partially etching of SiO2 technique.
28723346	4	38	dep	diffraction	776:786	arg1	study					788:792	study	788:792	study	788:792	Zinc crystal was also detected by X-ray diffraction study and HR-TEM observation.
28723346	4	38	dep	diffraction	776:786	arg1	observation					805:815	observation	805:815	observation	805:815	Zinc crystal was also detected by X-ray diffraction study and HR-TEM observation.
28723346	0	39	theme	chitosan/silica	48:62	arg1	membranes					64:72	chitosan/silica membranes	48:72	chitosan/silica membranes for efficient, recyclable catalysts used in ullmann reaction	48:133	Co-immobilization of Pd and Zn nanoparticles in chitosan/silica membranes for efficient, recyclable catalysts used in ullmann reaction.
28723346	3	40	theme	CS/SiO2	720:726	arg1	matrix					728:733	the CS/SiO2 matrix	716:733	the CS/SiO2 matrix	716:733	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	3	41	theme	catalyst	608:615	arg1	characterization					584:599	characterization	584:599	characterization of the catalyst	584:615	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	3	42	theme	photoelectron	550:562	arg1	XPS					578:580	XPS	578:580	XPS	578:580	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	3	42	theme	photoelectron	550:562	arg1	spectroscopy					564:575	X-ray photoelectron spectroscopy	544:575	X-ray photoelectron spectroscopy (XPS)	544:581	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	5	43	theme	membrane	845:852	arg1	catalyst					854:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst	818:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst	818:861	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	5	43	theme	membrane	845:852	arg1	active					873:878	active	873:878	active	873:878	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	3	44	dep	aggregates	671:680	arg1	10-15nm					689:695	10-15nm	689:695	10-15nm	689:695	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	4	45	theme	Zinc	736:739	arg1	crystal					741:747	Zinc crystal	736:747	Zinc crystal	736:747	Zinc crystal was also detected by X-ray diffraction study and HR-TEM observation.
28723346	6	46	theme	carrier	1144:1150	arg1	material					1152:1159	the same carrier material	1135:1159	the same carrier material	1135:1159	This work supplies a successful approach to realize Pd catalysts and Zn reducing reagent co-immobilized in the same carrier material with excellent catalytic performances.
28723346	5	47	theme	iodides	933:939	arg1	reactions					915:923	Ullmann reductive homocoupling reactions	884:923	Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides	884:957	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	6	48	theme	excellent	1166:1174	arg1	performances					1186:1197	excellent catalytic performances	1166:1197	excellent catalytic performances	1166:1197	This work supplies a successful approach to realize Pd catalysts and Zn reducing reagent co-immobilized in the same carrier material with excellent catalytic performances.
28723346	6	49	theme	successful	1049:1058	arg1	approach					1060:1067	a successful approach	1047:1067	a successful approach to realize Pd catalysts and Zn reducing reagent co-immobilized in the same carrier material with excellent catalytic performances	1047:1197	This work supplies a successful approach to realize Pd catalysts and Zn reducing reagent co-immobilized in the same carrier material with excellent catalytic performances.
28723346	6	50	theme	Pd	1080:1081	arg1	catalysts					1083:1091	Pd catalysts	1080:1091	Pd catalysts	1080:1091	This work supplies a successful approach to realize Pd catalysts and Zn reducing reagent co-immobilized in the same carrier material with excellent catalytic performances.
28723346	5	51	theme	aryl	928:931	arg1	iodides					933:939	aryl iodides	928:939	aryl iodides	928:939	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	5	52	theme	homocoupling	902:913	arg1	reactions					915:923	Ullmann reductive homocoupling reactions	884:923	Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides	884:957	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	3	53	theme	High-resolution	482:496	arg1	microscopy					520:529	High-resolution transmission electron microscopy	482:529	High-resolution transmission electron microscopy (HR-TEM)	482:538	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	3	53	theme	High-resolution	482:496	arg1	HR-TEM					532:537	HR-TEM	532:537	HR-TEM	532:537	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	1	54	dep	nanoparticles	226:238	arg1	supported					240:248	supported	240:248	nanoparticles supported on chitosan/silica (CS/SiO2) composite membrane is synthesized using partially etching of SiO2 technique	226:353	In this study, an efficient heterogeneous catalyst including palladium (Pd) and zinc (Zn) nanoparticles supported on chitosan/silica (CS/SiO2) composite membrane is synthesized using partially etching of SiO2 technique.
28723346	5	55	theme	aryl	945:948	arg1	bromides					950:957	aryl bromides	945:957	aryl bromides	945:957	The prepared Pd-Zn@CS/SiO2 membrane catalyst is highly active for Ullmann reductive homocoupling reactions of aryl iodides and aryl bromides, and can be recycled 5 times without significant loss of activities.
28723346	2	56	theme	N2	356:357	arg1	results					377:383	N2 sorption isotherm results	356:383	N2 sorption isotherm results	356:383	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	6	57	theme	reducing	1100:1107	arg1	reagent					1109:1115	Zn reducing reagent	1097:1115	Zn reducing reagent	1097:1115	This work supplies a successful approach to realize Pd catalysts and Zn reducing reagent co-immobilized in the same carrier material with excellent catalytic performances.
28723346	2	58	theme	surface	455:461	arg1	area					463:466	a BET surface area	449:466	a BET surface area of 26.50m2/g	449:479	N2 sorption isotherm results shows that the prepared Pd-Zn@CS/SiO2 (1/1) porous membrane had a BET surface area of 26.50m2/g.
28723346	0	59	theme	Pd	21:22	arg1	Co-immobilization					0:16	Co-immobilization	0:16	Co-immobilization of Pd and Zn	0:29	Co-immobilization of Pd and Zn nanoparticles in chitosan/silica membranes for efficient, recyclable catalysts used in ullmann reaction.
28723346	3	60	theme	X-ray	544:548	arg1	XPS					578:580	XPS	578:580	XPS	578:580	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
28723346	3	60	theme	X-ray	544:548	arg1	spectroscopy					564:575	X-ray photoelectron spectroscopy	544:575	X-ray photoelectron spectroscopy (XPS)	544:581	High-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), characterization of the catalyst shows that the Pd0 nanoparticles (below 5nm), and Zn0 aggregates (about 10-15nm) dispersed well in the CS/SiO2 matrix.
24820322	4	0	theme	electron	806:813	arg1	TEM					827:829	TEM	827:829	TEM	827:829	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	0	theme	electron	806:813	arg1	microscopy					815:824	transmission electron microscopy	793:824	transmission electron microscopy (TEM)	793:830	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	2	1	theme	hydroxide	421:429	arg1	solution					431:438	ammonium hydroxide solution	412:438	ammonium hydroxide solution	412:438	Magnetite nano-particles were synthesized by a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution.
24820322	6	2	theme	CMC	1116:1118	arg1	polymers					1120:1127	the natural CMC polymers	1104:1127	the natural CMC polymers	1104:1127	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	5	3	theme	average	981:987	arg1	size					989:992	an average size	978:992	an average size of 35nm	978:1000	As a result, magnetite nano-particles with an average size of 35nm were obtained.
24820322	2	4	theme	ammonium	412:419	arg1	solution					431:438	ammonium hydroxide solution	412:438	ammonium hydroxide solution	412:438	Magnetite nano-particles were synthesized by a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution.
24820322	6	5	theme	natural	1108:1114	arg1	polymers					1120:1127	the natural CMC polymers	1104:1127	the natural CMC polymers	1104:1127	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	2	6	from	tetrahydrate	364:375	arg1	solution					431:438	ammonium hydroxide solution	412:438	ammonium hydroxide solution	412:438	Magnetite nano-particles were synthesized by a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution.
24820322	3	7	with	Fe3O4	607:611	arg1	precursor					622:630	CMC precursor	618:630	CMC precursor	618:630	The M-CMC nano-composite particles were synthesized by embedding the magnetite nanoparticles inside carboxymethyl cellulose (CMC) using a freshly prepared mixture of Fe3O4 with CMC precursor.
24820322	3	8	theme	prepared	587:594	arg1	mixture					596:602	a freshly prepared mixture	577:602	a freshly prepared mixture of Fe3O4 with CMC precursor	577:630	The M-CMC nano-composite particles were synthesized by embedding the magnetite nanoparticles inside carboxymethyl cellulose (CMC) using a freshly prepared mixture of Fe3O4 with CMC precursor.
24820322	4	9	theme	X-ray	761:765	arg1	diffraction					774:784	X-ray powder diffraction	761:784	X-ray powder diffraction (XRD)	761:790	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	9	theme	X-ray	761:765	arg1	XRD					787:789	XRD	787:789	XRD	787:789	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	10	theme	transmission	793:804	arg1	TEM					827:829	TEM	827:829	TEM	827:829	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	10	theme	transmission	793:804	arg1	microscopy					815:824	transmission electron microscopy	793:824	transmission electron microscopy (TEM)	793:830	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	2	11	from	hexahydrate	397:407	arg1	solution					431:438	ammonium hydroxide solution	412:438	ammonium hydroxide solution	412:438	Magnetite nano-particles were synthesized by a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution.
24820322	2	12	theme	co-precipitation	317:332	arg1	method					334:339	a modified co-precipitation method	306:339	a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution	306:438	Magnetite nano-particles were synthesized by a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution.
24820322	5	13	theme	magnetite	948:956	arg1	result					940:945	a result	938:945	a result	938:945	As a result, magnetite nano-particles with an average size of 35nm were obtained.
24820322	5	13	theme	magnetite	948:956	arg1	nano-particles					958:971	magnetite nano-particles	948:971	magnetite nano-particles with an average size of 35nm	948:1000	As a result, magnetite nano-particles with an average size of 35nm were obtained.
24820322	1	14	theme	magnetite-carboxymethyl	181:203	arg1	material					238:245	magnetite-carboxymethyl cellulose nano-composite (M-CMC) material	181:245	magnetite-carboxymethyl cellulose nano-composite (M-CMC) material	181:245	The preparation and characterization of magnetite-carboxymethyl cellulose nano-composite (M-CMC) material is described.
24820322	4	15	theme	structural	664:673	arg1	properties					675:684	structural properties	664:684	structural properties	664:684	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	2	16	theme	modified	308:315	arg1	method					334:339	a modified co-precipitation method	306:339	a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution	306:438	Magnetite nano-particles were synthesized by a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution.
24820322	1	17	theme	cellulose	205:213	arg1	material					238:245	magnetite-carboxymethyl cellulose nano-composite (M-CMC) material	181:245	magnetite-carboxymethyl cellulose nano-composite (M-CMC) material	181:245	The preparation and characterization of magnetite-carboxymethyl cellulose nano-composite (M-CMC) material is described.
24820322	3	18	theme	nano-composite	451:464	arg1	particles					466:474	The M-CMC nano-composite particles	441:474	The M-CMC nano-composite particles	441:474	The M-CMC nano-composite particles were synthesized by embedding the magnetite nanoparticles inside carboxymethyl cellulose (CMC) using a freshly prepared mixture of Fe3O4 with CMC precursor.
24820322	6	19	theme	cellulose	1055:1063	arg1	particles					1080:1088	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles	1017:1088	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers	1017:1127	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	6	20	from	range	1146:1150	arg1	field					1181:1185	biomedical field	1170:1185	biomedical field	1170:1185	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	1	21	theme	nano-composite	215:228	arg1	material					238:245	magnetite-carboxymethyl cellulose nano-composite (M-CMC) material	181:245	magnetite-carboxymethyl cellulose nano-composite (M-CMC) material	181:245	The preparation and characterization of magnetite-carboxymethyl cellulose nano-composite (M-CMC) material is described.
24820322	6	22	theme	potential	1136:1144	arg1	range					1146:1150	a potential range	1134:1150	a potential range of application in biomedical field	1134:1185	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	3	23	theme	magnetite	510:518	arg1	nanoparticles					520:532	the magnetite nanoparticles	506:532	the magnetite nanoparticles inside carboxymethyl cellulose (CMC) using a freshly prepared mixture of Fe3O4 with CMC precursor	506:630	The M-CMC nano-composite particles were synthesized by embedding the magnetite nanoparticles inside carboxymethyl cellulose (CMC) using a freshly prepared mixture of Fe3O4 with CMC precursor.
24820322	0	24	theme	magnetite-carboxymethyl	29:51	arg1	cellulose					53:61	biocompatible magnetite-carboxymethyl cellulose	15:61	biocompatible magnetite-carboxymethyl cellulose	15:61	Preparation of biocompatible magnetite-carboxymethyl cellulose nanocomposite: characterization of nanocomposite by FTIR, XRD, FESEM and TEM.
24820322	6	25	from	application	1155:1165	arg1	field					1181:1185	biomedical field	1170:1185	biomedical field	1170:1185	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	2	26	theme	chloride	355:362	arg1	tetrahydrate					364:375	ferrous chloride tetrahydrate	347:375	ferrous chloride tetrahydrate	347:375	Magnetite nano-particles were synthesized by a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution.
24820322	6	27	from	field	1181:1185	arg1	range					1146:1150	a potential range	1134:1150	a potential range of application in biomedical field	1134:1185	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	1	28	theme	M-CMC	231:235	arg1	material					238:245	magnetite-carboxymethyl cellulose nano-composite (M-CMC) material	181:245	magnetite-carboxymethyl cellulose nano-composite (M-CMC) material	181:245	The preparation and characterization of magnetite-carboxymethyl cellulose nano-composite (M-CMC) material is described.
24820322	0	29	theme	biocompatible	15:27	arg1	cellulose					53:61	biocompatible magnetite-carboxymethyl cellulose	15:61	biocompatible magnetite-carboxymethyl cellulose	15:61	Preparation of biocompatible magnetite-carboxymethyl cellulose nanocomposite: characterization of nanocomposite by FTIR, XRD, FESEM and TEM.
24820322	6	30	theme	nano-composite	1065:1078	arg1	particles					1080:1088	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles	1017:1088	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers	1017:1127	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	2	31	theme	ferrous	347:353	arg1	tetrahydrate					364:375	ferrous chloride tetrahydrate	347:375	ferrous chloride tetrahydrate	347:375	Magnetite nano-particles were synthesized by a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution.
24820322	6	32	theme	application	1155:1165	arg1	range					1146:1150	a potential range	1134:1150	a potential range of application in biomedical field	1134:1185	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	4	33	theme	particle	645:652	arg1	size					654:657	particle size	645:657	particle size	645:657	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	3	34	theme	carboxymethyl	541:553	arg1	cellulose					555:563	carboxymethyl cellulose	541:563	carboxymethyl cellulose (CMC) using a freshly prepared mixture of Fe3O4 with CMC precursor	541:630	The M-CMC nano-composite particles were synthesized by embedding the magnetite nanoparticles inside carboxymethyl cellulose (CMC) using a freshly prepared mixture of Fe3O4 with CMC precursor.
24820322	3	34	theme	carboxymethyl	541:553	arg1	CMC					566:568	CMC	566:568	CMC	566:568	The M-CMC nano-composite particles were synthesized by embedding the magnetite nanoparticles inside carboxymethyl cellulose (CMC) using a freshly prepared mixture of Fe3O4 with CMC precursor.
24820322	6	35	theme	biomedical	1170:1179	arg1	field					1181:1185	biomedical field	1170:1185	biomedical field	1170:1185	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	1	36	theme	material	238:245	arg1	characterization					161:176	characterization	161:176	characterization	161:176	The preparation and characterization of magnetite-carboxymethyl cellulose nano-composite (M-CMC) material is described.
24820322	1	36	theme	material	238:245	arg1	preparation					145:155	preparation	145:155	preparation	145:155	The preparation and characterization of magnetite-carboxymethyl cellulose nano-composite (M-CMC) material is described.
24820322	0	37	theme	cellulose	53:61	arg1	Preparation					0:10	Preparation	0:10	Preparation of biocompatible magnetite-carboxymethyl cellulose	0:61	Preparation of biocompatible magnetite-carboxymethyl cellulose nanocomposite: characterization of nanocomposite by FTIR, XRD, FESEM and TEM.
24820322	6	38	theme	Fe3O4-carboxymethyl	1035:1053	arg1	particles					1080:1088	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles	1017:1088	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers	1017:1127	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	2	39	theme	Magnetite	261:269	arg1	nano-particles					271:284	Magnetite nano-particles	261:284	Magnetite nano-particles	261:284	Magnetite nano-particles were synthesized by a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution.
24820322	6	40	theme	biocompatible	1021:1033	arg1	particles					1080:1088	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles	1017:1088	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers	1017:1127	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	4	41	dep	infrared	853:860	arg1	FTIR					863:866	FTIR	863:866	FTIR	863:866	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	5	42	with	nano-particles	958:971	arg1	size					989:992	an average size	978:992	an average size of 35nm	978:1000	As a result, magnetite nano-particles with an average size of 35nm were obtained.
24820322	5	43	theme	35nm	997:1000	arg1	size					989:992	an average size	978:992	an average size of 35nm	978:1000	As a result, magnetite nano-particles with an average size of 35nm were obtained.
24820322	4	44	theme	scanning	888:895	arg1	FESEM					918:922	FESEM	918:922	FESEM	918:922	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	44	theme	scanning	888:895	arg1	microscopy					906:915	field emission scanning electron microscopy	873:915	field emission scanning electron microscopy (FESEM) analysis	873:932	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	45	theme	emission	879:886	arg1	FESEM					918:922	FESEM	918:922	FESEM	918:922	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	45	theme	emission	879:886	arg1	microscopy					906:915	field emission scanning electron microscopy	873:915	field emission scanning electron microscopy (FESEM) analysis	873:932	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	46	theme	microscopy	906:915	arg1	analysis					925:932	field emission scanning electron microscopy (FESEM) analysis	873:932	field emission scanning electron microscopy (FESEM) analysis	873:932	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	2	47	theme	chloride	388:395	arg1	hexahydrate					397:407	ferric chloride hexahydrate	381:407	ferric chloride hexahydrate	381:407	Magnetite nano-particles were synthesized by a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution.
24820322	1	48	dep	preparation	145:155	arg1	The					141:143	The	141:143	The	141:143	The preparation and characterization of magnetite-carboxymethyl cellulose nano-composite (M-CMC) material is described.
24820322	4	49	theme	magnetite-carboxymethyl	689:711	arg1	nano-composite					723:736	magnetite-carboxymethyl cellulose nano-composite	689:736	magnetite-carboxymethyl cellulose nano-composite	689:736	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	3	50	theme	CMC	618:620	arg1	precursor					622:630	CMC precursor	618:630	CMC precursor	618:630	The M-CMC nano-composite particles were synthesized by embedding the magnetite nanoparticles inside carboxymethyl cellulose (CMC) using a freshly prepared mixture of Fe3O4 with CMC precursor.
24820322	4	51	theme	field	873:877	arg1	FESEM					918:922	FESEM	918:922	FESEM	918:922	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	51	theme	field	873:877	arg1	microscopy					906:915	field emission scanning electron microscopy	873:915	field emission scanning electron microscopy (FESEM) analysis	873:932	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	2	52	theme	ferric	381:386	arg1	hexahydrate					397:407	ferric chloride hexahydrate	381:407	ferric chloride hexahydrate	381:407	Magnetite nano-particles were synthesized by a modified co-precipitation method using ferrous chloride tetrahydrate and ferric chloride hexahydrate in ammonium hydroxide solution.
24820322	6	53	contain	have	1129:1132	arg2	range					1146:1150	a potential range	1134:1150	a potential range of application in biomedical field	1134:1185	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	6	53	contain	have	1129:1132	arg1	particles					1080:1088	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles	1017:1088	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers	1017:1127	The biocompatible Fe3O4-carboxymethyl cellulose nano-composite particles obtained from the natural CMC polymers have a potential range of application in biomedical field.
24820322	0	54	theme	nanocomposite	98:110	arg1	characterization					78:93	characterization	78:93	characterization of nanocomposite	78:110	Preparation of biocompatible magnetite-carboxymethyl cellulose nanocomposite: characterization of nanocomposite by FTIR, XRD, FESEM and TEM.
24820322	4	55	theme	electron	897:904	arg1	FESEM					918:922	FESEM	918:922	FESEM	918:922	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	55	theme	electron	897:904	arg1	microscopy					906:915	field emission scanning electron microscopy	873:915	field emission scanning electron microscopy (FESEM) analysis	873:932	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	56	theme	powder	767:772	arg1	diffraction					774:784	X-ray powder diffraction	761:784	X-ray powder diffraction (XRD)	761:790	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	56	theme	powder	767:772	arg1	XRD					787:789	XRD	787:789	XRD	787:789	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	57	theme	nano-composite	723:736	arg1	properties					675:684	structural properties	664:684	structural properties	664:684	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	57	theme	nano-composite	723:736	arg1	Morphology					633:642	Morphology	633:642	Morphology	633:642	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	4	57	theme	nano-composite	723:736	arg1	size					654:657	particle size	645:657	particle size	645:657	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
24820322	3	58	theme	M-CMC	445:449	arg1	particles					466:474	The M-CMC nano-composite particles	441:474	The M-CMC nano-composite particles	441:474	The M-CMC nano-composite particles were synthesized by embedding the magnetite nanoparticles inside carboxymethyl cellulose (CMC) using a freshly prepared mixture of Fe3O4 with CMC precursor.
24820322	3	59	theme	Fe3O4	607:611	arg1	mixture					596:602	a freshly prepared mixture	577:602	a freshly prepared mixture of Fe3O4 with CMC precursor	577:630	The M-CMC nano-composite particles were synthesized by embedding the magnetite nanoparticles inside carboxymethyl cellulose (CMC) using a freshly prepared mixture of Fe3O4 with CMC precursor.
24820322	4	60	theme	cellulose	713:721	arg1	nano-composite					723:736	magnetite-carboxymethyl cellulose nano-composite	689:736	magnetite-carboxymethyl cellulose nano-composite	689:736	Morphology, particle size, and structural properties of magnetite-carboxymethyl cellulose nano-composite was accomplished using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR) and field emission scanning electron microscopy (FESEM) analysis.
28556245	5	0	theme	methoxyl	992:999	arg1	pectins					1001:1007	high and low methoxyl pectins	979:1007	high and low methoxyl pectins	979:1007	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	0	1	theme	fruit	78:82	arg1	esterification					52:65	esterification	52:65	esterification of passion fruit (Passiflora edulis f. flavicarpa)	52:116	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	8	2	theme	many	1408:1411	arg1	sectors					1424:1430	many industrial sectors	1408:1430	many industrial sectors	1408:1430	Considering the pseudoplastic properties of the flours, the application of both samples could be expanded to many industrial sectors.
28556245	0	3	theme	edulis	96:101	arg1	fruit					78:82	passion fruit	70:82	passion fruit (Passiflora edulis f. flavicarpa)	70:116	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	0	3	theme	edulis	96:101	arg1	flavicarpa					106:115	Passiflora edulis f. flavicarpa	85:115	Passiflora edulis f. flavicarpa	85:115	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	3	4	theme	food	627:630	arg1	industry					632:639	the food industry	623:639	the food industry	623:639	The aim of this study was to investigate the physico-chemical composition and physical properties of this material, which may be of interest to the food industry.
28556245	6	5	theme	fewer	1127:1131	arg1	tannins					1133:1139	60% fewer tannins	1123:1139	60% fewer tannins	1123:1139	CONCLUSION The flour obtained by maceration (FWT) offers greater benefits for industrial use, with 60% fewer tannins and greater thermal stability.
28556245	4	6	theme	flour	696:700	arg1	flour					732:736	flour	732:736	flour without treatment (FWOT)	732:761	RESULTS We obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT).
28556245	4	6	theme	flour	696:700	arg1	samples					666:672	two samples	662:672	two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour	662:771	RESULTS We obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT).
28556245	4	6	theme	flour	696:700	arg1	flour					767:771	flour	767:771	flour	767:771	RESULTS We obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT).
28556245	4	7	dep	RESULTS	642:648	arg1	obtained					653:660	obtained	653:660	obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT)	653:806	RESULTS We obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT).
28556245	2	8	theme	researchers	466:476	arg1	attention					453:461	the attention	449:461	the attention of researchers	449:476	For this reason, and also considering its importance as a source of functional components such as pectin, this organic waste is increasingly attracting the attention of researchers.
28556245	4	9	theme	peel	691:694	arg1	flour					696:700	passion fruit peel flour	677:700	passion fruit peel flour applying different processes	677:729	RESULTS We obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT).
28556245	5	10	theme	flour	831:835	arg1	samples					837:843	the flour samples	827:843	the flour samples	827:843	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	4	11	theme	different	711:719	arg1	processes					721:729	different processes	711:729	different processes	711:729	RESULTS We obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT).
28556245	3	12	dep	composition	541:551	arg1	the					520:522	the	520:522	the	520:522	The aim of this study was to investigate the physico-chemical composition and physical properties of this material, which may be of interest to the food industry.
28556245	1	13	theme	yellow	153:158	arg1	fruit					168:172	yellow passion fruit	153:172	yellow passion fruit	153:172	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	0	14	theme	f.	103:104	arg1	fruit					78:82	passion fruit	70:82	passion fruit (Passiflora edulis f. flavicarpa)	70:116	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	0	14	theme	f.	103:104	arg1	flavicarpa					106:115	Passiflora edulis f. flavicarpa	85:115	Passiflora edulis f. flavicarpa	85:115	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	5	15	theme	high	979:982	arg1	pectins					1001:1007	high and low methoxyl pectins	979:1007	high and low methoxyl pectins	979:1007	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	1	16	theme	passion	160:166	arg1	fruit					168:172	yellow passion fruit	153:172	yellow passion fruit	153:172	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	8	17	theme	industrial	1413:1422	arg1	sectors					1424:1430	many industrial sectors	1408:1430	many industrial sectors	1408:1430	Considering the pseudoplastic properties of the flours, the application of both samples could be expanded to many industrial sectors.
28556245	1	18	theme	fruit	168:172	arg1	peel					145:148	The peel	141:148	The peel of yellow passion fruit	141:172	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	0	19	theme	Physico-chemical	0:15	arg1	degree					42:47	degree	42:47	degree	42:47	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	0	19	theme	Physico-chemical	0:15	arg1	rheology					29:36	rheology	29:36	rheology	29:36	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	0	19	theme	Physico-chemical	0:15	arg1	properties					17:26	Physico-chemical properties	0:26	Physico-chemical properties	0:26	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	6	20	dep	CONCLUSION	1024:1033	arg1	offers					1074:1079	offers	1074:1079	offers	1074:1079	CONCLUSION The flour obtained by maceration (FWT) offers greater benefits for industrial use, with 60% fewer tannins and greater thermal stability.
28556245	8	21	theme	flours	1347:1352	arg1	properties					1329:1338	the pseudoplastic properties	1311:1338	the pseudoplastic properties of the flours	1311:1352	Considering the pseudoplastic properties of the flours, the application of both samples could be expanded to many industrial sectors.
28556245	6	22	theme	greater	1081:1087	arg1	benefits					1089:1096	greater benefits	1081:1096	greater benefits for industrial use	1081:1115	CONCLUSION The flour obtained by maceration (FWT) offers greater benefits for industrial use, with 60% fewer tannins and greater thermal stability.
28556245	1	23	dep	BACKGROUND	130:139	arg1	waste					199:203	an agro-industrial waste	180:203	an agro-industrial waste of great environmental impact	180:233	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	6	24	theme	industrial	1102:1111	arg1	use					1113:1115	industrial use	1102:1115	industrial use	1102:1115	CONCLUSION The flour obtained by maceration (FWT) offers greater benefits for industrial use, with 60% fewer tannins and greater thermal stability.
28556245	2	25	theme	functional	365:374	arg1	components					376:385	functional components	365:385	functional components such as pectin	365:400	For this reason, and also considering its importance as a source of functional components such as pectin, this organic waste is increasingly attracting the attention of researchers.
28556245	2	25	theme	functional	365:374	arg1	pectin					395:400	pectin	395:400	pectin	395:400	For this reason, and also considering its importance as a source of functional components such as pectin, this organic waste is increasingly attracting the attention of researchers.
28556245	2	26	theme	components	376:385	arg1	importance					339:348	its importance	335:348	its importance	335:348	For this reason, and also considering its importance as a source of functional components such as pectin, this organic waste is increasingly attracting the attention of researchers.
28556245	2	26	theme	components	376:385	arg1	source					355:360	a source	353:360	a source of functional components such as pectin	353:400	For this reason, and also considering its importance as a source of functional components such as pectin, this organic waste is increasingly attracting the attention of researchers.
28556245	5	27	theme	g	874:874	arg1	kg-1					876:879	372.4 g kg-1	868:879	372.4 g kg-1	868:879	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	4	28	theme	passion	677:683	arg1	flour					696:700	passion fruit peel flour	677:700	passion fruit peel flour applying different processes	677:729	RESULTS We obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT).
28556245	5	29	contain	contain	845:851	arg2	kg-1					876:879	372.4 g kg-1	868:879	372.4 g kg-1	868:879	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	5	29	contain	contain	845:851	arg1	samples					837:843	the flour samples	827:843	the flour samples	827:843	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	5	29	contain	contain	845:851	arg2	kg-1					891:894	246.7 kg-1	885:894	246.7 kg-1 of soluble fiber	885:911	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	5	30	theme	soluble	899:905	arg1	fiber					907:911	soluble fiber	899:911	soluble fiber	899:911	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	1	31	theme	agro-industrial	183:197	arg1	waste					199:203	an agro-industrial waste	180:203	an agro-industrial waste of great environmental impact	180:233	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	2	32	theme	organic	408:414	arg1	waste					416:420	this organic waste	403:420	this organic waste	403:420	For this reason, and also considering its importance as a source of functional components such as pectin, this organic waste is increasingly attracting the attention of researchers.
28556245	1	33	theme	total	270:274	arg1	weight					276:281	the total weight	266:281	the total weight of the fruit	266:294	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	3	34	theme	material	585:592	arg1	composition					541:551	physico-chemical composition	524:551	physico-chemical composition	524:551	The aim of this study was to investigate the physico-chemical composition and physical properties of this material, which may be of interest to the food industry.
28556245	3	34	theme	material	585:592	arg1	properties					566:575	physical properties	557:575	physical properties	557:575	The aim of this study was to investigate the physico-chemical composition and physical properties of this material, which may be of interest to the food industry.
28556245	5	35	theme	fiber	907:911	arg1	kg-1					891:894	246.7 kg-1	885:894	246.7 kg-1 of soluble fiber	885:911	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	5	35	theme	fiber	907:911	arg1	kg-1					876:879	372.4 g kg-1	868:879	372.4 g kg-1	868:879	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	4	36	theme	fruit	685:689	arg1	flour					696:700	passion fruit peel flour	677:700	passion fruit peel flour applying different processes	677:729	RESULTS We obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT).
28556245	0	37	theme	esterification	52:65	arg1	degree					42:47	degree	42:47	degree	42:47	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	0	37	theme	esterification	52:65	arg1	rheology					29:36	rheology	29:36	rheology	29:36	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	0	37	theme	esterification	52:65	arg1	properties					17:26	Physico-chemical properties	0:26	Physico-chemical properties	0:26	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	1	38	theme	weight	276:281	arg1	weight					276:281	the total weight	266:281	the total weight of the fruit	266:294	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	1	38	theme	weight	276:281	arg1	%					261:261	more than 50%	249:261	more than 50% of the total weight of the fruit	249:294	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	7	39	theme	dietary	1281:1287	arg1	products					1289:1296	dietary products	1281:1296	dietary products	1281:1296	In addition, this sample does not reabsorb moisture as easily, although FWOT also shows potential for use in dietary products.
28556245	8	40	theme	samples	1379:1385	arg1	application					1359:1369	the application	1355:1369	the application of both samples	1355:1385	Considering the pseudoplastic properties of the flours, the application of both samples could be expanded to many industrial sectors.
28556245	5	41	theme	low	988:990	arg1	pectins					1001:1007	high and low methoxyl pectins	979:1007	high and low methoxyl pectins	979:1007	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	8	42	theme	pseudoplastic	1315:1327	arg1	properties					1329:1338	the pseudoplastic properties	1311:1338	the pseudoplastic properties of the flours	1311:1352	Considering the pseudoplastic properties of the flours, the application of both samples could be expanded to many industrial sectors.
28556245	3	43	theme	physico-chemical	524:539	arg1	composition					541:551	physico-chemical composition	524:551	physico-chemical composition	524:551	The aim of this study was to investigate the physico-chemical composition and physical properties of this material, which may be of interest to the food industry.
28556245	6	44	theme	thermal	1153:1159	arg1	stability					1161:1169	greater thermal stability	1145:1169	greater thermal stability	1145:1169	CONCLUSION The flour obtained by maceration (FWT) offers greater benefits for industrial use, with 60% fewer tannins and greater thermal stability.
28556245	9	45	theme	Chemical	1451:1458	arg1	Industry					1460:1467	Chemical Industry	1451:1467	Chemical Industry	1451:1467	© 2017 Society of Chemical Industry.
28556245	3	46	theme	study	495:499	arg1	aim					483:485	The aim	479:485	The aim of this study	479:499	The aim of this study was to investigate the physico-chemical composition and physical properties of this material, which may be of interest to the food industry.
28556245	6	47	theme	greater	1145:1151	arg1	stability					1161:1169	greater thermal stability	1145:1169	greater thermal stability	1145:1169	CONCLUSION The flour obtained by maceration (FWT) offers greater benefits for industrial use, with 60% fewer tannins and greater thermal stability.
28556245	7	48	theme	reabsorb	1206:1213	arg1	moisture					1215:1222	reabsorb moisture	1206:1222	reabsorb moisture	1206:1222	In addition, this sample does not reabsorb moisture as easily, although FWOT also shows potential for use in dietary products.
28556245	4	49	dep	samples	666:672	arg1	flour					732:736	flour	732:736	flour without treatment (FWOT)	732:761	RESULTS We obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT).
28556245	4	49	dep	samples	666:672	arg1	samples					666:672	two samples	662:672	two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour	662:771	RESULTS We obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT).
28556245	4	49	dep	samples	666:672	arg1	flour					767:771	flour	767:771	flour	767:771	RESULTS We obtained two samples of passion fruit peel flour applying different processes: flour without treatment (FWOT) and flour with treatment by maceration (FWT).
28556245	1	50	theme	great	208:212	arg1	impact					228:233	great environmental impact	208:233	great environmental impact	208:233	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	0	51	theme	passion	70:76	arg1	fruit					78:82	passion fruit	70:82	passion fruit (Passiflora edulis f. flavicarpa)	70:116	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	0	51	theme	passion	70:76	arg1	flavicarpa					106:115	Passiflora edulis f. flavicarpa	85:115	Passiflora edulis f. flavicarpa	85:115	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	5	52	theme	FTIR	935:938	arg1	analysis					940:947	the FTIR analysis	931:947	the FTIR analysis	931:947	It was found that the flour samples contain, respectively, 372.4 g kg-1 and 246.7 kg-1 of soluble fiber and, according to the FTIR analysis, this material corresponds to high and low methoxyl pectins, respectively.
28556245	3	53	theme	physical	557:564	arg1	properties					566:575	physical properties	557:575	physical properties	557:575	The aim of this study was to investigate the physico-chemical composition and physical properties of this material, which may be of interest to the food industry.
28556245	1	54	theme	environmental	214:226	arg1	impact					228:233	great environmental impact	208:233	great environmental impact	208:233	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	1	55	theme	fruit	290:294	arg1	weight					276:281	the total weight	266:281	the total weight of the fruit	266:294	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	1	56	theme	impact	228:233	arg1	waste					199:203	an agro-industrial waste	180:203	an agro-industrial waste of great environmental impact	180:233	BACKGROUND The peel of yellow passion fruit is as an agro-industrial waste of great environmental impact, representing more than 50% of the total weight of the fruit.
28556245	7	57	from	potential	1260:1268	arg1	products					1289:1296	dietary products	1281:1296	dietary products	1281:1296	In addition, this sample does not reabsorb moisture as easily, although FWOT also shows potential for use in dietary products.
28556245	0	58	theme	Passiflora	85:94	arg1	fruit					78:82	passion fruit	70:82	passion fruit (Passiflora edulis f. flavicarpa)	70:116	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
28556245	0	58	theme	Passiflora	85:94	arg1	flavicarpa					106:115	Passiflora edulis f. flavicarpa	85:115	Passiflora edulis f. flavicarpa	85:115	Physico-chemical properties, rheology and degree of esterification of passion fruit (Passiflora edulis f. flavicarpa) peel flour.
26053018	0	0	theme	Influenza	90:98	arg1	Virus					102:106	Influenza A Virus	90:106	Influenza A Virus in the Murine Model	90:126	The Intranasal Application of Zanamivir and Carrageenan Is Synergistically Active against Influenza A Virus in the Murine Model.
26053018	2	1	theme	respiratory	503:513	arg1	infections					515:524	respiratory infections	503:524	respiratory infections	503:524	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	7	2	dep	CONCLUSION	1311:1320	arg1	tested					1393:1398	tested	1393:1398	should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials	1373:1473	CONCLUSION A nasal spray containing carrageenan and Zanamivir should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials.
26053018	1	3	theme	infections	248:257	arg1	treatment					205:213	the treatment	201:213	the treatment of viral upper respiratory tract infections	201:257	BACKGROUND Carrageenan is a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections.
26053018	5	4	dep	virus	952:956	arg1	HA					959:960	HA	959:960	HA closely related to the avian influenza A(H7N9) virus	959:1013	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	2	5	with	compound	412:419	arg1	polymer					455:461	the broadly active antiviral polymer	426:461	the broadly active antiviral polymer	426:461	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	6	6	dep	72	1286:1287	arg1	to					1283:1284	to	1283:1284	to	1283:1284	Remarkably, this benefit is maintained even when the treatment starts up to 72 hours post infection.
26053018	5	7	theme	lethal	902:907	arg1	model					919:923	a lethal influenza model	900:923	a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo	900:1207	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	5	8	theme	infected	1142:1149	arg1	animals					1151:1157	infected animals	1142:1157	infected animals	1142:1157	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	3	9	theme	specific	556:563	arg1	Zanamivir					585:593	the specific anti-influenza drug Zanamivir	552:593	the specific anti-influenza drug Zanamivir	552:593	Thus, the combination of the specific anti-influenza drug Zanamivir together with carrageenan in a formulation suitable for intranasal application was evaluated in-vitro and in-vivo.
26053018	4	10	theme	several	805:811	arg1	strains					831:837	several influenza A virus strains	805:837	several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7)	805:869	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	5	11	theme	animals	1151:1157	arg1	survival					1130:1137	survival	1130:1137	survival of infected animals	1130:1157	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	0	12	theme	A	100:100	arg1	Virus					102:106	Influenza A Virus	90:106	Influenza A Virus in the Murine Model	90:126	The Intranasal Application of Zanamivir and Carrageenan Is Synergistically Active against Influenza A Virus in the Murine Model.
26053018	5	13	theme	pdm	1030:1032	arg1	virus					1044:1048	a H1N1(09)pdm influenza virus	1020:1048	a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo	1020:1207	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	3	14	theme	anti-influenza	565:578	arg1	Zanamivir					585:593	the specific anti-influenza drug Zanamivir	552:593	the specific anti-influenza drug Zanamivir	552:593	Thus, the combination of the specific anti-influenza drug Zanamivir together with carrageenan in a formulation suitable for intranasal application was evaluated in-vitro and in-vivo.
26053018	2	15	contain	has	463:465	arg1	combination					371:381	the combination	367:381	the combination of a specific anti-influenza compound with the broadly active antiviral polymer	367:461	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	2	15	contain	has	463:465	arg2	potential					472:480	huge potential	467:480	huge potential for the treatment of respiratory infections	467:524	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	2	16	theme	influenza	284:292	arg1	virus					294:298	influenza virus	284:298	influenza virus	284:298	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	5	17	theme	influenza	909:917	arg1	model					919:923	a lethal influenza model	900:923	a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo	900:1207	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	2	18	theme	specific	388:395	arg1	compound					412:419	a specific anti-influenza compound	386:419	a specific anti-influenza compound with the broadly active antiviral polymer	386:461	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	1	19	theme	proven	168:173	arg1	compound					188:195	a clinically proven and marketed compound	155:195	a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections	155:257	BACKGROUND Carrageenan is a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections.
26053018	1	19	theme	proven	168:173	arg1	Carrageenan					140:150	BACKGROUND Carrageenan	129:150	BACKGROUND Carrageenan	129:150	BACKGROUND Carrageenan is a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections.
26053018	5	20	theme	low	932:934	arg1	virus					952:956	a low pathogenic H7N7 virus	930:956	a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus)	930:1014	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	5	21	theme	influenza	1034:1042	arg1	virus					1044:1048	a H1N1(09)pdm influenza virus	1020:1048	a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo	1020:1207	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	2	22	theme	anti-influenza	397:410	arg1	compound					412:419	a specific anti-influenza compound	386:419	a specific anti-influenza compound with the broadly active antiviral polymer	386:461	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	0	23	theme	Intranasal	4:13	arg1	Application					15:25	The Intranasal Application	0:25	The Intranasal Application of Zanamivir and Carrageenan	0:54	The Intranasal Application of Zanamivir and Carrageenan Is Synergistically Active against Influenza A Virus in the Murine Model.
26053018	0	23	theme	Intranasal	4:13	arg1	Active					75:80	Active	75:80	Active	75:80	The Intranasal Application of Zanamivir and Carrageenan Is Synergistically Active against Influenza A Virus in the Murine Model.
26053018	7	24	contain	containing	1336:1345	arg1	spray					1330:1334	A nasal spray	1322:1334	A nasal spray containing carrageenan and Zanamivir	1322:1371	CONCLUSION A nasal spray containing carrageenan and Zanamivir should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials.
26053018	7	24	contain	containing	1336:1345	arg2	Zanamivir					1363:1371	Zanamivir	1363:1371	Zanamivir	1363:1371	CONCLUSION A nasal spray containing carrageenan and Zanamivir should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials.
26053018	7	24	contain	containing	1336:1345	arg2	carrageenan					1347:1357	carrageenan	1347:1357	carrageenan	1347:1357	CONCLUSION A nasal spray containing carrageenan and Zanamivir should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials.
26053018	5	25	theme	H7N7	947:950	arg1	virus					952:956	a low pathogenic H7N7 virus	930:956	a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus)	930:1014	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	4	26	dep	strains	831:837	arg1	09					845:846	09	845:846	09	845:846	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	4	26	dep	strains	831:837	arg1	H7N7					865:868	H7N7	865:868	H7N7	865:868	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	4	26	dep	strains	831:837	arg1	H1N1					840:843	H1N1	840:843	H1N1	840:843	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	4	26	dep	strains	831:837	arg1	H3N2					853:856	H3N2	853:856	H3N2	853:856	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	4	26	dep	strains	831:837	arg1	H5N1					859:862	H5N1	859:862	H5N1	859:862	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	1	27	theme	marketed	179:186	arg1	compound					188:195	a clinically proven and marketed compound	155:195	a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections	155:257	BACKGROUND Carrageenan is a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections.
26053018	1	27	theme	marketed	179:186	arg1	Carrageenan					140:150	BACKGROUND Carrageenan	129:150	BACKGROUND Carrageenan	129:150	BACKGROUND Carrageenan is a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections.
26053018	5	28	theme	compounds	1096:1104	arg1	use					1084:1086	the combined use	1071:1086	the combined use of both compounds	1071:1104	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	5	29	with	model	919:923	arg1	virus					1044:1048	a H1N1(09)pdm influenza virus	1020:1048	a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo	1020:1207	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	5	29	with	model	919:923	arg1	virus					952:956	a low pathogenic H7N7 virus	930:956	a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus)	930:1014	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	7	30	theme	influenza	1446:1454	arg1	prevention					1404:1413	prevention	1404:1413	prevention	1404:1413	CONCLUSION A nasal spray containing carrageenan and Zanamivir should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials.
26053018	7	30	theme	influenza	1446:1454	arg1	treatment					1419:1427	treatment	1419:1427	treatment	1419:1427	CONCLUSION A nasal spray containing carrageenan and Zanamivir should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials.
26053018	5	31	theme	pathogenic	936:945	arg1	virus					952:956	a low pathogenic H7N7 virus	930:956	a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus)	930:1014	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	3	32	from	carrageenan	609:619	arg1	formulation					626:636	a formulation	624:636	a formulation suitable for intranasal application	624:672	Thus, the combination of the specific anti-influenza drug Zanamivir together with carrageenan in a formulation suitable for intranasal application was evaluated in-vitro and in-vivo.
26053018	4	33	theme	PRINCIPAL	710:718	arg1	FINDINGS					720:727	PRINCIPAL FINDINGS	710:727	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).	710:870	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	7	34	from	prevention	1404:1413	arg1	trials					1468:1473	clinical trials	1459:1473	clinical trials	1459:1473	CONCLUSION A nasal spray containing carrageenan and Zanamivir should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials.
26053018	5	35	theme	avian	985:989	arg1	virus					1009:1013	the avian influenza A(H7N9) virus	981:1013	the avian influenza A(H7N9) virus	981:1013	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	3	36	theme	drug	580:583	arg1	Zanamivir					585:593	the specific anti-influenza drug Zanamivir	552:593	the specific anti-influenza drug Zanamivir	552:593	Thus, the combination of the specific anti-influenza drug Zanamivir together with carrageenan in a formulation suitable for intranasal application was evaluated in-vitro and in-vivo.
26053018	2	37	theme	active	438:443	arg1	polymer					455:461	the broadly active antiviral polymer	426:461	the broadly active antiviral polymer	426:461	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	2	38	theme	antiviral	445:453	arg1	polymer					455:461	the broadly active antiviral polymer	426:461	the broadly active antiviral polymer	426:461	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	0	39	theme	Murine	115:120	arg1	Model					122:126	the Murine Model	111:126	the Murine Model	111:126	The Intranasal Application of Zanamivir and Carrageenan Is Synergistically Active against Influenza A Virus in the Murine Model.
26053018	5	40	theme	H1N1	1022:1025	arg1	virus					1044:1048	a H1N1(09)pdm influenza virus	1020:1048	a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo	1020:1207	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	3	41	theme	Zanamivir	585:593	arg1	combination					537:547	the combination	533:547	the combination of the specific anti-influenza drug Zanamivir together with carrageenan in a formulation suitable for intranasal application	533:672	Thus, the combination of the specific anti-influenza drug Zanamivir together with carrageenan in a formulation suitable for intranasal application was evaluated in-vitro and in-vivo.
26053018	7	42	from	treatment	1419:1427	arg1	trials					1468:1473	clinical trials	1459:1473	clinical trials	1459:1473	CONCLUSION A nasal spray containing carrageenan and Zanamivir should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials.
26053018	2	43	with	infections	325:334	arg1	viruses					359:365	other respiratory viruses	341:365	other respiratory viruses	341:365	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	5	44	theme	A	1001:1001	arg1	virus					1009:1013	the avian influenza A(H7N9) virus	981:1013	the avian influenza A(H7N9) virus	981:1013	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	4	45	dep	H1N1	840:843	arg1	pdm					848:850	pdm	848:850	pdm	848:850	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	2	46	theme	compound	412:419	arg1	combination					371:381	the combination	367:381	the combination of a specific anti-influenza compound with the broadly active antiviral polymer	367:461	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	5	47	theme	combined	1075:1082	arg1	use					1084:1086	the combined use	1071:1086	the combined use of both compounds	1071:1104	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	0	48	theme	Zanamivir	30:38	arg1	Application					15:25	The Intranasal Application	0:25	The Intranasal Application of Zanamivir and Carrageenan	0:54	The Intranasal Application of Zanamivir and Carrageenan Is Synergistically Active against Influenza A Virus in the Murine Model.
26053018	0	48	theme	Zanamivir	30:38	arg1	Active					75:80	Active	75:80	Active	75:80	The Intranasal Application of Zanamivir and Carrageenan Is Synergistically Active against Influenza A Virus in the Murine Model.
26053018	4	49	theme	A	823:823	arg1	strains					831:837	several influenza A virus strains	805:837	several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7)	805:869	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	7	50	theme	nasal	1324:1328	arg1	spray					1330:1334	A nasal spray	1322:1334	A nasal spray containing carrageenan and Zanamivir	1322:1371	CONCLUSION A nasal spray containing carrageenan and Zanamivir should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials.
26053018	5	51	theme	H7N9	1003:1006	arg1	virus					1009:1013	the avian influenza A(H7N9) virus	981:1013	the avian influenza A(H7N9) virus	981:1013	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	2	52	theme	respiratory	347:357	arg1	viruses					359:365	other respiratory viruses	341:365	other respiratory viruses	341:365	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	0	53	theme	Carrageenan	44:54	arg1	Application					15:25	The Intranasal Application	0:25	The Intranasal Application of Zanamivir and Carrageenan	0:54	The Intranasal Application of Zanamivir and Carrageenan Is Synergistically Active against Influenza A Virus in the Murine Model.
26053018	0	53	theme	Carrageenan	44:54	arg1	Active					75:80	Active	75:80	Active	75:80	The Intranasal Application of Zanamivir and Carrageenan Is Synergistically Active against Influenza A Virus in the Murine Model.
26053018	7	54	theme	uncomplicated	1432:1444	arg1	influenza					1446:1454	uncomplicated influenza	1432:1454	uncomplicated influenza	1432:1454	CONCLUSION A nasal spray containing carrageenan and Zanamivir should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials.
26053018	2	55	theme	other	341:345	arg1	viruses					359:365	other respiratory viruses	341:365	other respiratory viruses	341:365	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	5	56	from	virus	1044:1048	arg1	mice					1061:1064	C57BL/6 mice	1053:1064	C57BL/6 mice	1053:1064	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	4	57	dep	FINDINGS	720:727	arg1	show					732:735	show	732:735	show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7)	732:869	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	3	58	theme	intranasal	651:660	arg1	application					662:672	intranasal application	651:672	intranasal application	651:672	Thus, the combination of the specific anti-influenza drug Zanamivir together with carrageenan in a formulation suitable for intranasal application was evaluated in-vitro and in-vivo.
26053018	5	59	theme	09	1027:1028	arg1	virus					1044:1048	a H1N1(09)pdm influenza virus	1020:1048	a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo	1020:1207	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	4	60	theme	influenza	813:821	arg1	strains					831:837	several influenza A virus strains	805:837	several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7)	805:869	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	1	61	theme	viral	218:222	arg1	infections					248:257	viral upper respiratory tract infections	218:257	viral upper respiratory tract infections	218:257	BACKGROUND Carrageenan is a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections.
26053018	5	62	theme	related	970:976	arg1	HA					959:960	HA	959:960	HA closely related to the avian influenza A(H7N9) virus	959:1013	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	0	63	from	Virus	102:106	arg1	Model					122:126	the Murine Model	111:126	the Murine Model	111:126	The Intranasal Application of Zanamivir and Carrageenan Is Synergistically Active against Influenza A Virus in the Murine Model.
26053018	4	64	theme	virus	825:829	arg1	strains					831:837	several influenza A virus strains	805:837	several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7)	805:869	PRINCIPAL FINDINGS We show in-vitro that carrageenan and Zanamivir act synergistically against several influenza A virus strains (H1N1(09)pdm, H3N2, H5N1, H7N7).
26053018	1	65	theme	BACKGROUND	129:138	arg1	compound					188:195	a clinically proven and marketed compound	155:195	a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections	155:257	BACKGROUND Carrageenan is a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections.
26053018	1	65	theme	BACKGROUND	129:138	arg1	Carrageenan					140:150	BACKGROUND Carrageenan	129:150	BACKGROUND Carrageenan	129:150	BACKGROUND Carrageenan is a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections.
26053018	1	66	theme	upper	224:228	arg1	infections					248:257	viral upper respiratory tract infections	218:257	viral upper respiratory tract infections	218:257	BACKGROUND Carrageenan is a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections.
26053018	7	67	theme	clinical	1459:1466	arg1	trials					1468:1473	clinical trials	1459:1473	clinical trials	1459:1473	CONCLUSION A nasal spray containing carrageenan and Zanamivir should therefore be tested for prevention and treatment of uncomplicated influenza in clinical trials.
26053018	3	68	theme	suitable	638:645	arg1	formulation					626:636	a formulation	624:636	a formulation suitable for intranasal application	624:672	Thus, the combination of the specific anti-influenza drug Zanamivir together with carrageenan in a formulation suitable for intranasal application was evaluated in-vitro and in-vivo.
26053018	5	69	from	virus	952:956	arg1	mice					1061:1064	C57BL/6 mice	1053:1064	C57BL/6 mice	1053:1064	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	1	70	theme	respiratory	230:240	arg1	infections					248:257	viral upper respiratory tract infections	218:257	viral upper respiratory tract infections	218:257	BACKGROUND Carrageenan is a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections.
26053018	2	71	theme	infections	515:524	arg1	treatment					490:498	the treatment	486:498	the treatment of respiratory infections	486:524	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	5	72	theme	C57BL/6	1053:1059	arg1	mice					1061:1064	C57BL/6 mice	1053:1064	C57BL/6 mice	1053:1064	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	5	73	theme	influenza	991:999	arg1	virus					1009:1013	the avian influenza A(H7N9) virus	981:1013	the avian influenza A(H7N9) virus	981:1013	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	5	74	with	comparison	1162:1171	arg1	placebo					1201:1207	placebo	1201:1207	placebo	1201:1207	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	5	74	with	comparison	1162:1171	arg1	mono-therapies					1183:1196	mono-therapies	1183:1196	mono-therapies	1183:1196	Moreover, we demonstrate in a lethal influenza model with a low pathogenic H7N7 virus (HA closely related to the avian influenza A(H7N9) virus) and a H1N1(09)pdm influenza virus in C57BL/6 mice that the combined use of both compounds significantly increases survival of infected animals in comparison with both mono-therapies or placebo.
26053018	2	75	theme	huge	467:470	arg1	potential					472:480	huge potential	467:480	huge potential for the treatment of respiratory infections	467:524	As infections caused by influenza virus are often accompanied by infections with other respiratory viruses the combination of a specific anti-influenza compound with the broadly active antiviral polymer has huge potential for the treatment of respiratory infections.
26053018	1	76	theme	tract	242:246	arg1	infections					248:257	viral upper respiratory tract infections	218:257	viral upper respiratory tract infections	218:257	BACKGROUND Carrageenan is a clinically proven and marketed compound for the treatment of viral upper respiratory tract infections.
26491307	4	0	theme	faster	495:500	arg1	clotting					508:515	faster blood clotting	495:515	faster blood clotting	495:515	The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO.
26491307	7	1	from	skin	1154:1157	arg1	analysis					1102:1109	The qualitative analysis	1086:1109	The qualitative analysis of ex-vivo re-epithelialization on porcine skin	1086:1157	The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
26491307	7	2	from	analysis	1102:1109	arg1	skin					1154:1157	porcine skin	1146:1157	porcine skin	1146:1157	The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
26491307	7	3	theme	wound	1201:1205	arg1	area					1207:1210	wound area	1201:1210	wound area	1201:1210	The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
26491307	5	4	theme	prepared	596:603	arg1	bandages					615:622	The prepared composite bandages	592:622	The prepared composite bandages	592:622	The prepared composite bandages exhibited excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and methicillin resistant S. aureus (MRSA).
26491307	6	5	dep	evaluation	796:805	arg1	studies					913:919	studies	913:919	studies	913:919	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	4	6	theme	clotting	508:515	arg1	ability					517:523	controlled degradation profile and faster blood clotting ability	460:523	controlled degradation profile and faster blood clotting ability	460:523	The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO.
26491307	7	7	theme	keratinocyte	1168:1179	arg1	infiltration					1181:1192	keratinocyte infiltration	1168:1192	keratinocyte infiltration toward wound area for nZnO alginate bandages	1168:1237	The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
26491307	5	8	theme	composite	605:613	arg1	bandages					615:622	The prepared composite bandages	592:622	The prepared composite bandages	592:622	The prepared composite bandages exhibited excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and methicillin resistant S. aureus (MRSA).
26491307	4	9	theme	degradation	471:481	arg1	profile					483:489	controlled degradation profile	460:489	controlled degradation profile	460:489	The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO.
26491307	7	10	theme	nZnO	1216:1219	arg1	bandages					1230:1237	nZnO alginate bandages	1216:1237	nZnO alginate bandages	1216:1237	The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
26491307	1	11	theme	composite	139:147	arg1	bandage					149:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel.
26491307	4	12	theme	controlled	460:469	arg1	profile					483:489	controlled degradation profile	460:489	controlled degradation profile	460:489	The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO.
26491307	5	13	theme	excellent	634:642	arg1	activity					658:665	excellent antimicrobial activity	634:665	excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and methicillin resistant S. aureus (MRSA)	634:775	The prepared composite bandages exhibited excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and methicillin resistant S. aureus (MRSA).
26491307	6	14	theme	bandages	833:840	arg1	infiltration					900:911	infiltration	900:911	infiltration	900:911	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	6	14	theme	bandages	833:840	arg1	evaluation					796:805	Cytocompatibility evaluation	778:805	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay	778:894	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	1	15	theme	freeze-dry	174:183	arg1	method					185:190	freeze-dry method	174:190	freeze-dry method from the mixture of nZnO and alginate hydrogel	174:237	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel.
26491307	3	16	theme	swelling	324:331	arg1	ratios					333:338	The swelling ratios	320:338	The swelling ratios of the bandages	320:354	The swelling ratios of the bandages decreased with increasing concentrations of nZnO.
26491307	5	17	theme	antimicrobial	644:656	arg1	activity					658:665	excellent antimicrobial activity	634:665	excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and methicillin resistant S. aureus (MRSA)	634:775	The prepared composite bandages exhibited excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and methicillin resistant S. aureus (MRSA).
26491307	6	18	theme	composite	823:831	arg1	bandages					833:840	the prepared composite bandages	810:840	the prepared composite bandages done on human dermal fibroblast cells by Alamar assay	810:894	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	2	19	with	porous	276:281	arg1	porosity					288:295	porosity	288:295	porosity	288:295	The developed composite bandage was porous with porosity at a range of 60%-70%.
26491307	7	20	theme	porcine	1146:1152	arg1	skin					1154:1157	porcine skin	1146:1157	porcine skin	1146:1157	The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
26491307	6	21	contain	have	946:949	arg2	nature					963:968	a non-toxic nature	951:968	a non-toxic nature	951:968	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	6	21	contain	have	946:949	arg1	bandages					937:944	the bandages	933:944	the bandages	933:944	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	4	22	theme	profile	483:489	arg1	ability					517:523	controlled degradation profile and faster blood clotting ability	460:523	controlled degradation profile and faster blood clotting ability	460:523	The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO.
26491307	6	23	theme	prepared	814:821	arg1	bandages					833:840	the prepared composite bandages	810:840	the prepared composite bandages done on human dermal fibroblast cells by Alamar assay	810:894	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	1	24	theme	Alginate	89:96	arg1	bandage					149:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel.
26491307	7	25	theme	qualitative	1090:1100	arg1	analysis					1102:1109	The qualitative analysis	1086:1109	The qualitative analysis of ex-vivo re-epithelialization on porcine skin	1086:1157	The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
26491307	6	26	theme	slight	1010:1015	arg1	reduction					1017:1025	slight reduction	1010:1025	slight reduction in viability	1010:1038	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	6	27	theme	dermal	856:861	arg1	cells					874:878	human dermal fibroblast cells	850:878	human dermal fibroblast cells	850:878	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	1	28	theme	hydrogel/zinc	98:110	arg1	bandage					149:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel.
26491307	0	29	theme	hydrogel/nano	24:36	arg1	composite					49:57	alginate hydrogel/nano zinc oxide composite	15:57	alginate hydrogel/nano zinc oxide composite	15:57	Exploration of alginate hydrogel/nano zinc oxide composite bandages for infected wounds.
26491307	4	30	theme	KALTOSTAT®	546:555	arg1	bandages					569:576	the KALTOSTAT® and control bandages	542:576	bandages	569:576	The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO.
26491307	7	31	theme	ex-vivo	1114:1120	arg1	re-epithelialization					1122:1141	ex-vivo re-epithelialization	1114:1141	ex-vivo re-epithelialization on porcine skin	1114:1157	The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
26491307	1	32	theme	oxide	112:116	arg1	bandage					149:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel.
26491307	6	33	theme	human	850:854	arg1	cells					874:878	human dermal fibroblast cells	850:878	human dermal fibroblast cells	850:878	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	4	34	theme	control	561:567	arg1	bandages					569:576	the KALTOSTAT® and control bandages	542:576	bandages	569:576	The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO.
26491307	0	35	theme	alginate	15:22	arg1	composite					49:57	alginate hydrogel/nano zinc oxide composite	15:57	alginate hydrogel/nano zinc oxide composite	15:57	Exploration of alginate hydrogel/nano zinc oxide composite bandages for infected wounds.
26491307	6	36	theme	nZnO	997:1000	arg1	concentrations					979:992	lower concentrations	973:992	lower concentrations of nZnO	973:1000	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	7	37	theme	re-epithelialization	1122:1141	arg1	analysis					1102:1109	The qualitative analysis	1086:1109	The qualitative analysis of ex-vivo re-epithelialization on porcine skin	1086:1157	The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
26491307	4	38	theme	nZnO	434:437	arg1	incorporation					439:451	nZnO incorporation	434:451	nZnO incorporation	434:451	The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO.
26491307	1	39	theme	nanoparticles	118:130	arg1	bandage					149:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel.
26491307	7	40	theme	alginate	1221:1228	arg1	bandages					1230:1237	nZnO alginate bandages	1216:1237	nZnO alginate bandages	1216:1237	The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
26491307	3	41	theme	bandages	347:354	arg1	ratios					333:338	The swelling ratios	320:338	The swelling ratios of the bandages	320:354	The swelling ratios of the bandages decreased with increasing concentrations of nZnO.
26491307	0	42	theme	oxide	43:47	arg1	composite					49:57	alginate hydrogel/nano zinc oxide composite	15:57	alginate hydrogel/nano zinc oxide composite	15:57	Exploration of alginate hydrogel/nano zinc oxide composite bandages for infected wounds.
26491307	2	43	theme	%	317:317	arg1	range					302:306	a range	300:306	a range of 60%-70%	300:317	The developed composite bandage was porous with porosity at a range of 60%-70%.
26491307	1	44	theme	nZnO	212:215	arg1	mixture					201:207	the mixture	197:207	the mixture of nZnO and alginate hydrogel	197:237	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel.
26491307	0	45	theme	zinc	38:41	arg1	composite					49:57	alginate hydrogel/nano zinc oxide composite	15:57	alginate hydrogel/nano zinc oxide composite	15:57	Exploration of alginate hydrogel/nano zinc oxide composite bandages for infected wounds.
26491307	3	46	theme	nZnO	400:403	arg1	concentrations					382:395	increasing concentrations	371:395	increasing concentrations of nZnO	371:403	The swelling ratios of the bandages decreased with increasing concentrations of nZnO.
26491307	3	47	theme	increasing	371:380	arg1	concentrations					382:395	increasing concentrations	371:395	increasing concentrations of nZnO	371:403	The swelling ratios of the bandages decreased with increasing concentrations of nZnO.
26491307	6	48	theme	lower	973:977	arg1	concentrations					979:992	lower concentrations	973:992	lower concentrations of nZnO	973:1000	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	6	49	theme	Alamar	883:888	arg1	assay					890:894	Alamar assay	883:894	Alamar assay	883:894	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	6	50	theme	Cytocompatibility	778:794	arg1	evaluation					796:805	Cytocompatibility evaluation	778:805	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay	778:894	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	0	51	theme	composite	49:57	arg1	Exploration					0:10	Exploration	0:10	Exploration of alginate hydrogel/nano zinc oxide composite	0:57	Exploration of alginate hydrogel/nano zinc oxide composite bandages for infected wounds.
26491307	6	52	theme	non-toxic	953:961	arg1	nature					963:968	a non-toxic nature	951:968	a non-toxic nature	951:968	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	6	53	from	reduction	1017:1025	arg1	viability					1030:1038	viability	1030:1038	viability	1030:1038	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	1	54	from	mixture	201:207	arg1	method					185:190	freeze-dry method	174:190	freeze-dry method from the mixture of nZnO and alginate hydrogel	174:237	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel.
26491307	1	55	theme	nZnO	133:136	arg1	bandage					149:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage	89:155	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel.
26491307	5	56	theme	Candida	716:722	arg1	albicans					724:731	Candida albicans	716:731	Candida albicans	716:731	The prepared composite bandages exhibited excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and methicillin resistant S. aureus (MRSA).
26491307	6	57	theme	increasing	1054:1063	arg1	concentrations					1070:1083	increasing nZnO concentrations	1054:1083	increasing nZnO concentrations	1054:1083	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	1	58	theme	alginate	221:228	arg1	hydrogel					230:237	alginate hydrogel	221:237	alginate hydrogel	221:237	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel.
26491307	7	59	from	re-epithelialization	1122:1141	arg1	skin					1154:1157	porcine skin	1146:1157	porcine skin	1146:1157	The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
26491307	6	60	theme	fibroblast	863:872	arg1	cells					874:878	human dermal fibroblast cells	850:878	human dermal fibroblast cells	850:878	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	2	61	theme	%	313:313	arg1	%					317:317	60%-70%	311:317	60%-70%	311:317	The developed composite bandage was porous with porosity at a range of 60%-70%.
26491307	1	62	theme	hydrogel	230:237	arg1	mixture					201:207	the mixture	197:207	the mixture of nZnO and alginate hydrogel	197:237	Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel.
26491307	0	63	theme	infected	72:79	arg1	wounds					81:86	infected wounds	72:86	infected wounds	72:86	Exploration of alginate hydrogel/nano zinc oxide composite bandages for infected wounds.
26491307	4	64	theme	composite	410:418	arg1	bandages					420:427	The composite bandages	406:427	The composite bandages with nZnO incorporation	406:451	The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO.
26491307	2	65	theme	composite	254:262	arg1	porous					276:281	porous	276:281	porous	276:281	The developed composite bandage was porous with porosity at a range of 60%-70%.
26491307	2	65	theme	composite	254:262	arg1	bandage					264:270	The developed composite bandage	240:270	The developed composite bandage	240:270	The developed composite bandage was porous with porosity at a range of 60%-70%.
26491307	4	66	with	bandages	420:427	arg1	incorporation					439:451	nZnO incorporation	434:451	nZnO incorporation	434:451	The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO.
26491307	4	67	theme	blood	502:506	arg1	clotting					508:515	faster blood clotting	495:515	faster blood clotting	495:515	The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO.
26491307	2	68	theme	developed	244:252	arg1	porous					276:281	porous	276:281	porous	276:281	The developed composite bandage was porous with porosity at a range of 60%-70%.
26491307	2	68	theme	developed	244:252	arg1	bandage					264:270	The developed composite bandage	240:270	The developed composite bandage	240:270	The developed composite bandage was porous with porosity at a range of 60%-70%.
26491307	6	69	theme	nZnO	1065:1068	arg1	concentrations					1070:1083	increasing nZnO concentrations	1054:1083	increasing nZnO concentrations	1054:1083	Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations.
26491307	2	70	from	range	302:306	arg1	porous					276:281	porous	276:281	porous	276:281	The developed composite bandage was porous with porosity at a range of 60%-70%.
26491307	2	70	from	range	302:306	arg1	bandage					264:270	The developed composite bandage	240:270	The developed composite bandage	240:270	The developed composite bandage was porous with porosity at a range of 60%-70%.
26014750	8	0	theme	Multi-functional	1339:1354	arg1	hydrogels					1360:1368	Multi-functional PVA hydrogels	1339:1368	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity	1339:1442	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	5	1	theme	final	884:888	arg1	PVA					890:892	a final PVA	882:892	a final PVA	882:892	Both heparin and gelatin were covalently incorporated into PVA at an equal ratio resulting in a final PVA:heparin:gelatin composition of 19:0.5:0.5.
26014750	1	2	theme	cell	150:153	arg1	environment					155:165	the natural in vivo cell environment	130:165	the natural in vivo cell environment within artificial implants	130:192	The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants, has recently been identified as crucial for achieving complex cellular functions.
26014750	1	3	theme	complex	249:255	arg1	functions					266:274	complex cellular functions	249:274	complex cellular functions	249:274	The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants, has recently been identified as crucial for achieving complex cellular functions.
26014750	7	4	theme	analogues	1181:1189	arg1	combination					1162:1172	this combination	1157:1172	this combination of ECM analogues	1157:1189	Most importantly, this combination of ECM analogues supplemented PVA with the dual functionalities of promoting cellular adhesion and sequestering growth factors essential for cellular proliferation.
26014750	4	5	theme	hydrogels	777:785	arg1	permselectivity					738:752	the permselectivity	734:752	the permselectivity of UV photopolymerized hydrogels	734:785	PVA was functionalized with 7 and 20 methacrylate functional groups per chain (FG/c) to tailor the permselectivity of UV photopolymerized hydrogels.
26014750	2	6	theme	synthetic	343:351	arg1	network					353:359	a largely synthetic network	333:359	a largely synthetic network	333:359	The incorporation of two or more biological cues within a largely synthetic network can provide a simplified model of multifunctional ECM presentation to encapsulated cells.
26014750	1	7	dep	in	142:143	arg1	vivo					145:148	vivo	145:148	vivo	145:148	The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants, has recently been identified as crucial for achieving complex cellular functions.
26014750	1	8	theme	cellular	257:264	arg1	functions					266:274	complex cellular functions	249:274	complex cellular functions	249:274	The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants, has recently been identified as crucial for achieving complex cellular functions.
26014750	3	9	theme	biological	573:582	arg1	gelatin					607:613	gelatin	607:613	gelatin	607:613	Therefore, the aim of this study was to examine the effects of simultaneously and covalently incorporating two dissimilar biological molecules, heparin and gelatin, within a PVA hydrogel.
26014750	3	9	theme	biological	573:582	arg1	heparin					595:601	heparin	595:601	heparin	595:601	Therefore, the aim of this study was to examine the effects of simultaneously and covalently incorporating two dissimilar biological molecules, heparin and gelatin, within a PVA hydrogel.
26014750	3	9	theme	biological	573:582	arg1	molecules					584:592	two dissimilar biological molecules	558:592	two dissimilar biological molecules	558:592	Therefore, the aim of this study was to examine the effects of simultaneously and covalently incorporating two dissimilar biological molecules, heparin and gelatin, within a PVA hydrogel.
26014750	6	10	theme	PVA	1061:1063	arg1	characteristics					1070:1084	the PVA base characteristics	1057:1084	the PVA base characteristics including its permselectivity to different proteins	1057:1136	The combination of both heparin and gelatin within a PVA network has proven to be stable over time without compromising the PVA base characteristics including its permselectivity to different proteins.
26014750	6	10	theme	PVA	1061:1063	arg1	permselectivity					1100:1114	its permselectivity	1096:1114	its permselectivity to different proteins	1096:1136	The combination of both heparin and gelatin within a PVA network has proven to be stable over time without compromising the PVA base characteristics including its permselectivity to different proteins.
26014750	3	11	theme	study	478:482	arg1	aim					466:468	the aim	462:468	the aim of this study	462:482	Therefore, the aim of this study was to examine the effects of simultaneously and covalently incorporating two dissimilar biological molecules, heparin and gelatin, within a PVA hydrogel.
26014750	4	12	theme	functional	689:698	arg1	groups					700:705	7 and 20 methacrylate functional groups	667:705	7 and 20 methacrylate functional groups per chain (FG/c)	667:722	PVA was functionalized with 7 and 20 methacrylate functional groups per chain (FG/c) to tailor the permselectivity of UV photopolymerized hydrogels.
26014750	7	13	theme	cellular	1315:1322	arg1	proliferation					1324:1336	cellular proliferation	1315:1336	cellular proliferation	1315:1336	Most importantly, this combination of ECM analogues supplemented PVA with the dual functionalities of promoting cellular adhesion and sequestering growth factors essential for cellular proliferation.
26014750	4	14	theme	photopolymerized	760:775	arg1	hydrogels					777:785	UV photopolymerized hydrogels	757:785	UV photopolymerized hydrogels	757:785	PVA was functionalized with 7 and 20 methacrylate functional groups per chain (FG/c) to tailor the permselectivity of UV photopolymerized hydrogels.
26014750	1	15	theme	artificial	174:183	arg1	implants					185:192	artificial implants	174:192	artificial implants	174:192	The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants, has recently been identified as crucial for achieving complex cellular functions.
26014750	8	16	theme	PVA	1356:1358	arg1	hydrogels					1360:1368	Multi-functional PVA hydrogels	1339:1368	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity	1339:1442	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	6	17	theme	heparin	961:967	arg1	combination					941:951	The combination	937:951	The combination of both heparin and gelatin within a PVA network	937:1000	The combination of both heparin and gelatin within a PVA network has proven to be stable over time without compromising the PVA base characteristics including its permselectivity to different proteins.
26014750	6	17	theme	heparin	961:967	arg1	stable					1019:1024	stable	1019:1024	stable	1019:1024	The combination of both heparin and gelatin within a PVA network has proven to be stable over time without compromising the PVA base characteristics including its permselectivity to different proteins.
26014750	4	18	theme	UV	757:758	arg1	hydrogels					777:785	UV photopolymerized hydrogels	757:785	UV photopolymerized hydrogels	757:785	PVA was functionalized with 7 and 20 methacrylate functional groups per chain (FG/c) to tailor the permselectivity of UV photopolymerized hydrogels.
26014750	8	19	from	potential	1449:1457	arg1	applications					1481:1492	various biomedical applications	1462:1492	various biomedical applications including artificial cell implants	1462:1527	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	8	19	from	potential	1449:1457	arg1	implants					1520:1527	artificial cell implants	1504:1527	artificial cell implants	1504:1527	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	5	20	theme	equal	857:861	arg1	ratio					863:867	an equal ratio	854:867	an equal ratio resulting in a final PVA	854:892	Both heparin and gelatin were covalently incorporated into PVA at an equal ratio resulting in a final PVA:heparin:gelatin composition of 19:0.5:0.5.
26014750	8	21	theme	biomedical	1470:1479	arg1	applications					1481:1492	various biomedical applications	1462:1492	various biomedical applications including artificial cell implants	1462:1527	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	8	21	theme	biomedical	1470:1479	arg1	implants					1520:1527	artificial cell implants	1504:1527	artificial cell implants	1504:1527	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	2	22	theme	biological	310:319	arg1	cues					321:324	two or more biological cues	298:324	two or more biological cues	298:324	The incorporation of two or more biological cues within a largely synthetic network can provide a simplified model of multifunctional ECM presentation to encapsulated cells.
26014750	2	23	theme	simplified	375:384	arg1	model					386:390	a simplified model	373:390	a simplified model of multifunctional ECM presentation to encapsulated cells	373:448	The incorporation of two or more biological cues within a largely synthetic network can provide a simplified model of multifunctional ECM presentation to encapsulated cells.
26014750	3	24	theme	dissimilar	562:571	arg1	gelatin					607:613	gelatin	607:613	gelatin	607:613	Therefore, the aim of this study was to examine the effects of simultaneously and covalently incorporating two dissimilar biological molecules, heparin and gelatin, within a PVA hydrogel.
26014750	3	24	theme	dissimilar	562:571	arg1	heparin					595:601	heparin	595:601	heparin	595:601	Therefore, the aim of this study was to examine the effects of simultaneously and covalently incorporating two dissimilar biological molecules, heparin and gelatin, within a PVA hydrogel.
26014750	3	24	theme	dissimilar	562:571	arg1	molecules					584:592	two dissimilar biological molecules	558:592	two dissimilar biological molecules	558:592	Therefore, the aim of this study was to examine the effects of simultaneously and covalently incorporating two dissimilar biological molecules, heparin and gelatin, within a PVA hydrogel.
26014750	1	25	theme	multiple	89:96	arg1	cues					109:112	multiple biological cues	89:112	multiple biological cues	89:112	The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants, has recently been identified as crucial for achieving complex cellular functions.
26014750	0	26	theme	PVA	29:31	arg1	Bioactivity					0:10	Bioactivity	0:10	Bioactivity of permselective PVA	0:31	Bioactivity of permselective PVA hydrogels with mixed ECM analogues.
26014750	8	27	theme	artificial	1504:1513	arg1	implants					1520:1527	artificial cell implants	1504:1527	artificial cell implants	1504:1527	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	5	28	theme	gelatin	902:908	arg1	composition					910:920	gelatin composition	902:920	gelatin composition of 19:0.5:0.5	902:934	Both heparin and gelatin were covalently incorporated into PVA at an equal ratio resulting in a final PVA:heparin:gelatin composition of 19:0.5:0.5.
26014750	1	29	theme	biological	98:107	arg1	cues					109:112	multiple biological cues	89:112	multiple biological cues	89:112	The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants, has recently been identified as crucial for achieving complex cellular functions.
26014750	2	30	theme	encapsulated	431:442	arg1	cells					444:448	encapsulated cells	431:448	encapsulated cells	431:448	The incorporation of two or more biological cues within a largely synthetic network can provide a simplified model of multifunctional ECM presentation to encapsulated cells.
26014750	8	31	theme	controlled	1389:1398	arg1	characteristics					1408:1422	synthetically controlled network characteristics	1375:1422	synthetically controlled network characteristics	1375:1422	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	1	32	theme	cues	109:112	arg1	presentation					73:84	The presentation	69:84	The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants,	69:193	The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants, has recently been identified as crucial for achieving complex cellular functions.
26014750	8	33	from	applications	1481:1492	arg1	potential					1449:1457	potential	1449:1457	potential	1449:1457	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	2	34	theme	presentation	415:426	arg1	model					386:390	a simplified model	373:390	a simplified model of multifunctional ECM presentation to encapsulated cells	373:448	The incorporation of two or more biological cues within a largely synthetic network can provide a simplified model of multifunctional ECM presentation to encapsulated cells.
26014750	7	35	theme	essential	1301:1309	arg1	factors					1293:1299	growth factors	1286:1299	growth factors essential for cellular proliferation	1286:1336	Most importantly, this combination of ECM analogues supplemented PVA with the dual functionalities of promoting cellular adhesion and sequestering growth factors essential for cellular proliferation.
26014750	8	36	theme	cell	1515:1518	arg1	implants					1520:1527	artificial cell implants	1504:1527	artificial cell implants	1504:1527	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	2	37	theme	ECM	411:413	arg1	presentation					415:426	multifunctional ECM presentation	395:426	multifunctional ECM presentation to encapsulated cells	395:448	The incorporation of two or more biological cues within a largely synthetic network can provide a simplified model of multifunctional ECM presentation to encapsulated cells.
26014750	4	38	theme	methacrylate	676:687	arg1	groups					700:705	7 and 20 methacrylate functional groups	667:705	7 and 20 methacrylate functional groups per chain (FG/c)	667:722	PVA was functionalized with 7 and 20 methacrylate functional groups per chain (FG/c) to tailor the permselectivity of UV photopolymerized hydrogels.
26014750	6	39	theme	gelatin	973:979	arg1	combination					941:951	The combination	937:951	The combination of both heparin and gelatin within a PVA network	937:1000	The combination of both heparin and gelatin within a PVA network has proven to be stable over time without compromising the PVA base characteristics including its permselectivity to different proteins.
26014750	6	39	theme	gelatin	973:979	arg1	stable					1019:1024	stable	1019:1024	stable	1019:1024	The combination of both heparin and gelatin within a PVA network has proven to be stable over time without compromising the PVA base characteristics including its permselectivity to different proteins.
26014750	2	40	theme	multifunctional	395:409	arg1	presentation					415:426	multifunctional ECM presentation	395:426	multifunctional ECM presentation to encapsulated cells	395:448	The incorporation of two or more biological cues within a largely synthetic network can provide a simplified model of multifunctional ECM presentation to encapsulated cells.
26014750	0	41	theme	ECM	54:56	arg1	analogues					58:66	mixed ECM analogues	48:66	mixed ECM analogues	48:66	Bioactivity of permselective PVA hydrogels with mixed ECM analogues.
26014750	8	42	theme	various	1462:1468	arg1	applications					1481:1492	various biomedical applications	1462:1492	various biomedical applications including artificial cell implants	1462:1527	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	8	42	theme	various	1462:1468	arg1	implants					1520:1527	artificial cell implants	1504:1527	artificial cell implants	1504:1527	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	8	43	theme	network	1400:1406	arg1	characteristics					1408:1422	synthetically controlled network characteristics	1375:1422	synthetically controlled network characteristics	1375:1422	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	0	44	theme	mixed	48:52	arg1	analogues					58:66	mixed ECM analogues	48:66	mixed ECM analogues	48:66	Bioactivity of permselective PVA hydrogels with mixed ECM analogues.
26014750	7	45	theme	ECM	1177:1179	arg1	analogues					1181:1189	ECM analogues	1177:1189	ECM analogues	1177:1189	Most importantly, this combination of ECM analogues supplemented PVA with the dual functionalities of promoting cellular adhesion and sequestering growth factors essential for cellular proliferation.
26014750	7	46	theme	growth	1286:1291	arg1	factors					1293:1299	growth factors	1286:1299	growth factors essential for cellular proliferation	1286:1336	Most importantly, this combination of ECM analogues supplemented PVA with the dual functionalities of promoting cellular adhesion and sequestering growth factors essential for cellular proliferation.
26014750	7	47	theme	cellular	1251:1258	arg1	adhesion					1260:1267	cellular adhesion	1251:1267	cellular adhesion	1251:1267	Most importantly, this combination of ECM analogues supplemented PVA with the dual functionalities of promoting cellular adhesion and sequestering growth factors essential for cellular proliferation.
26014750	5	48	theme	19:0.5:0.5	925:934	arg1	composition					910:920	gelatin composition	902:920	gelatin composition of 19:0.5:0.5	902:934	Both heparin and gelatin were covalently incorporated into PVA at an equal ratio resulting in a final PVA:heparin:gelatin composition of 19:0.5:0.5.
26014750	3	49	theme	PVA	625:627	arg1	hydrogel					629:636	a PVA hydrogel	623:636	a PVA hydrogel	623:636	Therefore, the aim of this study was to examine the effects of simultaneously and covalently incorporating two dissimilar biological molecules, heparin and gelatin, within a PVA hydrogel.
26014750	2	50	theme	cues	321:324	arg1	incorporation					281:293	The incorporation	277:293	The incorporation of two or more biological cues within a largely synthetic network	277:359	The incorporation of two or more biological cues within a largely synthetic network can provide a simplified model of multifunctional ECM presentation to encapsulated cells.
26014750	6	51	theme	base	1065:1068	arg1	characteristics					1070:1084	the PVA base characteristics	1057:1084	the PVA base characteristics including its permselectivity to different proteins	1057:1136	The combination of both heparin and gelatin within a PVA network has proven to be stable over time without compromising the PVA base characteristics including its permselectivity to different proteins.
26014750	6	51	theme	base	1065:1068	arg1	permselectivity					1100:1114	its permselectivity	1096:1114	its permselectivity to different proteins	1096:1136	The combination of both heparin and gelatin within a PVA network has proven to be stable over time without compromising the PVA base characteristics including its permselectivity to different proteins.
26014750	5	52	dep	incorporated	829:840	arg1	heparin					894:900	heparin	894:900	heparin	894:900	Both heparin and gelatin were covalently incorporated into PVA at an equal ratio resulting in a final PVA:heparin:gelatin composition of 19:0.5:0.5.
26014750	5	52	dep	incorporated	829:840	arg1	composition					910:920	gelatin composition	902:920	gelatin composition of 19:0.5:0.5	902:934	Both heparin and gelatin were covalently incorporated into PVA at an equal ratio resulting in a final PVA:heparin:gelatin composition of 19:0.5:0.5.
26014750	1	53	theme	natural	134:140	arg1	environment					155:165	the natural in vivo cell environment	130:165	the natural in vivo cell environment within artificial implants	130:192	The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants, has recently been identified as crucial for achieving complex cellular functions.
26014750	6	54	theme	PVA	990:992	arg1	network					994:1000	a PVA network	988:1000	a PVA network	988:1000	The combination of both heparin and gelatin within a PVA network has proven to be stable over time without compromising the PVA base characteristics including its permselectivity to different proteins.
26014750	7	55	theme	dual	1217:1220	arg1	functionalities					1222:1236	the dual functionalities	1213:1236	the dual functionalities of promoting cellular adhesion and sequestering growth factors essential for cellular proliferation	1213:1336	Most importantly, this combination of ECM analogues supplemented PVA with the dual functionalities of promoting cellular adhesion and sequestering growth factors essential for cellular proliferation.
26014750	6	56	theme	different	1119:1127	arg1	proteins					1129:1136	different proteins	1119:1136	different proteins	1119:1136	The combination of both heparin and gelatin within a PVA network has proven to be stable over time without compromising the PVA base characteristics including its permselectivity to different proteins.
26014750	0	57	theme	permselective	15:27	arg1	PVA					29:31	permselective PVA	15:31	permselective PVA	15:31	Bioactivity of permselective PVA hydrogels with mixed ECM analogues.
26014750	1	58	theme	in	142:143	arg1	environment					155:165	the natural in vivo cell environment	130:165	the natural in vivo cell environment within artificial implants	130:192	The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants, has recently been identified as crucial for achieving complex cellular functions.
26014750	8	59	with	hydrogels	1360:1368	arg1	characteristics					1408:1422	synthetically controlled network characteristics	1375:1422	synthetically controlled network characteristics	1375:1422	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
26014750	8	59	with	hydrogels	1360:1368	arg1	permselectivity					1428:1442	permselectivity	1428:1442	permselectivity	1428:1442	Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.
27083375	0	0	theme	regeneration	73:84	arg1	potential					102:110	high sorption, regeneration and application potential	58:110	high sorption, regeneration and application potential	58:110	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.
27083375	6	1	theme	consecutive	853:863	arg1	cycles					865:870	20 consecutive cycles	850:870	20 consecutive cycles	850:870	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	5	2	theme	g	788:788	arg1	capacities					753:762	the maximum sorption capacities	732:762	the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively	732:806	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	1	3	theme	novel	152:156	arg1	composite					187:195	a novel and environmentally friendly composite	150:195	a novel and environmentally friendly composite	150:195	This study was undertaken to prepare a novel and environmentally friendly composite for the use in the wastewater treatment process.
27083375	0	4	theme	application	90:100	arg1	potential					102:110	high sorption, regeneration and application potential	58:110	high sorption, regeneration and application potential	58:110	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.
27083375	5	5	theme	Equilibrium	621:631	arg1	experiments					642:652	Equilibrium sorption experiments	621:652	Equilibrium sorption experiments	621:652	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	3	6	theme	contaminated	506:517	arg1	media					519:523	Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media	464:523	Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media	464:523	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	7	7	theme	real	983:986	arg1	wastewater					988:997	real wastewater	983:997	real wastewater without performance decrease	983:1026	More importantly, CAC could also be used in the treatment of real wastewater without performance decrease.
27083375	3	8	theme	mode	362:365	arg1	potential					382:390	dynamic flow mode decolorization potential	349:390	dynamic flow mode decolorization potential	349:390	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	5	9	theme	sorption	633:640	arg1	experiments					642:652	Equilibrium sorption experiments	621:652	Equilibrium sorption experiments	621:652	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	6	10	from	regeneration	814:825	arg1	cycles					865:870	20 consecutive cycles	850:870	20 consecutive cycles	850:870	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	4	11	theme	contact	546:552	arg1	time					554:557	contact time	546:557	contact time	546:557	pH, sorbent dosage, contact time and flow rate were screened through the sorption experiments.
27083375	5	12	theme	RR2	710:712	arg1	dyes					722:725	RR2 and AR1 dyes	710:725	RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively	710:806	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	6	13	theme	reuse	831:835	arg1	advantages					892:901	other important advantages	876:901	other important advantages of this composite	876:919	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	6	13	theme	reuse	831:835	arg1	regeneration					814:825	Good regeneration	809:825	Good regeneration	809:825	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	6	13	theme	reuse	831:835	arg1	potential					837:845	reuse potential	831:845	reuse potential	831:845	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	8	14	theme	dyes	1117:1120	arg1	removal					1098:1104	the removal	1094:1104	the removal of anionic dyes from aqueous solutions	1094:1143	Overall, this study suggests that CAC is a promising sorbent for the removal of anionic dyes from aqueous solutions.
27083375	5	15	theme	sorption	744:751	arg1	capacities					753:762	the maximum sorption capacities	732:762	the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively	732:806	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	3	16	theme	dynamic	349:355	arg1	potential					382:390	dynamic flow mode decolorization potential	349:390	dynamic flow mode decolorization potential	349:390	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	8	17	from	solutions	1135:1143	arg1	removal					1098:1104	the removal	1094:1104	the removal of anionic dyes from aqueous solutions	1094:1143	Overall, this study suggests that CAC is a promising sorbent for the removal of anionic dyes from aqueous solutions.
27083375	5	18	theme	maximum	736:742	arg1	capacities					753:762	the maximum sorption capacities	732:762	the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively	732:806	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	1	19	theme	environmentally	162:176	arg1	composite					187:195	a novel and environmentally friendly composite	150:195	a novel and environmentally friendly composite	150:195	This study was undertaken to prepare a novel and environmentally friendly composite for the use in the wastewater treatment process.
27083375	5	20	theme	462.74	767:772	arg1	capacities					753:762	the maximum sorption capacities	732:762	the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively	732:806	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	5	21	theme	AR1	718:720	arg1	dyes					722:725	RR2 and AR1 dyes	710:725	RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively	710:806	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	1	22	theme	friendly	178:185	arg1	composite					187:195	a novel and environmentally friendly composite	150:195	a novel and environmentally friendly composite	150:195	This study was undertaken to prepare a novel and environmentally friendly composite for the use in the wastewater treatment process.
27083375	0	23	theme	Chitosan-alunite	0:15	arg1	composite					17:25	Chitosan-alunite composite	0:25	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.	0:111	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.
27083375	2	24	theme	glucosamine	305:315	arg1	chitosan					329:336	chitosan	329:336	chitosan	329:336	This composite was produced by immobilizing alunite with a glucosamine biopolymer, chitosan.
27083375	2	24	theme	glucosamine	305:315	arg1	biopolymer					317:326	a glucosamine biopolymer	303:326	a glucosamine biopolymer	303:326	This composite was produced by immobilizing alunite with a glucosamine biopolymer, chitosan.
27083375	8	25	theme	promising	1072:1080	arg1	CAC					1063:1065	CAC	1063:1065	CAC	1063:1065	Overall, this study suggests that CAC is a promising sorbent for the removal of anionic dyes from aqueous solutions.
27083375	8	25	theme	promising	1072:1080	arg1	sorbent					1082:1088	a promising sorbent	1070:1088	a promising sorbent for the removal of anionic dyes from aqueous solutions	1070:1143	Overall, this study suggests that CAC is a promising sorbent for the removal of anionic dyes from aqueous solutions.
27083375	3	26	theme	decolorization	367:380	arg1	potential					382:390	dynamic flow mode decolorization potential	349:390	dynamic flow mode decolorization potential	349:390	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	6	27	theme	composite	911:919	arg1	potential					837:845	reuse potential	831:845	reuse potential	831:845	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	6	27	theme	composite	911:919	arg1	regeneration					814:825	Good regeneration	809:825	Good regeneration	809:825	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	6	27	theme	composite	911:919	arg1	advantages					892:901	other important advantages	876:901	other important advantages of this composite	876:919	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	3	28	theme	Red	469:471	arg1	Acid					464:467	Acid Red 1	464:473	Acid Red 1 (AR1)	464:479	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	3	28	theme	Red	469:471	arg1	AR1					476:478	AR1	476:478	AR1	476:478	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	5	29	theme	mg	785:786	arg1	-1					790:791	-1	790:791	-1	790:791	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	5	29	theme	mg	785:786	arg1	g					788:788	588.75 mg g	778:788	588.75 mg g(-1)	778:792	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	4	30	theme	sorbent	530:536	arg1	dosage					538:543	sorbent dosage	530:543	sorbent dosage	530:543	pH, sorbent dosage, contact time and flow rate were screened through the sorption experiments.
27083375	7	31	theme	performance	1007:1017	arg1	decrease					1019:1026	performance decrease	1007:1026	performance decrease	1007:1026	More importantly, CAC could also be used in the treatment of real wastewater without performance decrease.
27083375	6	32	theme	Good	809:812	arg1	potential					837:845	reuse potential	831:845	reuse potential	831:845	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	6	32	theme	Good	809:812	arg1	regeneration					814:825	Good regeneration	809:825	Good regeneration	809:825	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	6	32	theme	Good	809:812	arg1	advantages					892:901	other important advantages	876:901	other important advantages of this composite	876:919	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	0	33	theme	dye	41:43	arg1	remover					45:51	An effective dye remover	28:51	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.	0:111	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.
27083375	5	34	contain	has	673:675	arg2	potential					696:704	very high sorption potential	677:704	very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively	677:806	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	5	34	contain	has	673:675	arg1	CAC					669:671	CAC	669:671	CAC	669:671	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	5	35	theme	high	682:685	arg1	potential					696:704	very high sorption potential	677:704	very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively	677:806	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	4	36	theme	sorption	599:606	arg1	experiments					608:618	the sorption experiments	595:618	the sorption experiments	595:618	pH, sorbent dosage, contact time and flow rate were screened through the sorption experiments.
27083375	0	37	theme	effective	31:39	arg1	remover					45:51	An effective dye remover	28:51	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.	0:111	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.
27083375	5	38	theme	sorption	687:694	arg1	potential					696:704	very high sorption potential	677:704	very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively	677:806	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	1	39	from	use	205:207	arg1	process					237:243	the wastewater treatment process	212:243	the wastewater treatment process	212:243	This study was undertaken to prepare a novel and environmentally friendly composite for the use in the wastewater treatment process.
27083375	3	40	theme	Acid	464:467	arg1	media					519:523	Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media	464:523	Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media	464:523	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	3	41	theme	chitosan-alunite	399:414	arg1	composite					416:424	the chitosan-alunite composite	395:424	the chitosan-alunite composite (CAC)	395:430	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	3	41	theme	chitosan-alunite	399:414	arg1	CAC					427:429	CAC	427:429	CAC	427:429	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	3	42	theme	flow	357:360	arg1	potential					382:390	dynamic flow mode decolorization potential	349:390	dynamic flow mode decolorization potential	349:390	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	8	43	theme	aqueous	1127:1133	arg1	solutions					1135:1143	aqueous solutions	1127:1143	aqueous solutions	1127:1143	Overall, this study suggests that CAC is a promising sorbent for the removal of anionic dyes from aqueous solutions.
27083375	7	44	theme	wastewater	988:997	arg1	treatment					970:978	the treatment	966:978	the treatment of real wastewater without performance decrease	966:1026	More importantly, CAC could also be used in the treatment of real wastewater without performance decrease.
27083375	3	45	theme	composite	416:424	arg1	Batch					339:343	Batch	339:343	Batch	339:343	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	3	45	theme	composite	416:424	arg1	potential					382:390	dynamic flow mode decolorization potential	349:390	dynamic flow mode decolorization potential	349:390	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	6	46	theme	other	876:880	arg1	potential					837:845	reuse potential	831:845	reuse potential	831:845	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	6	46	theme	other	876:880	arg1	regeneration					814:825	Good regeneration	809:825	Good regeneration	809:825	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	6	46	theme	other	876:880	arg1	advantages					892:901	other important advantages	876:901	other important advantages of this composite	876:919	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	6	47	from	potential	837:845	arg1	cycles					865:870	20 consecutive cycles	850:870	20 consecutive cycles	850:870	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	4	48	theme	flow	563:566	arg1	rate					568:571	flow rate	563:571	flow rate	563:571	pH, sorbent dosage, contact time and flow rate were screened through the sorption experiments.
27083375	7	49	used	used	958:961	arg2	CAC					940:942	CAC	940:942	CAC	940:942	More importantly, CAC could also be used in the treatment of real wastewater without performance decrease.
27083375	1	50	theme	wastewater	216:225	arg1	process					237:243	the wastewater treatment process	212:243	the wastewater treatment process	212:243	This study was undertaken to prepare a novel and environmentally friendly composite for the use in the wastewater treatment process.
27083375	0	51	theme	sorption	63:70	arg1	potential					102:110	high sorption, regeneration and application potential	58:110	high sorption, regeneration and application potential	58:110	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.
27083375	0	52	dep	composite	17:25	arg1	remover					45:51	An effective dye remover	28:51	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.	0:111	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.
27083375	0	53	with	remover	45:51	arg1	potential					102:110	high sorption, regeneration and application potential	58:110	high sorption, regeneration and application potential	58:110	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.
27083375	3	54	theme	2	498:498	arg1	media					519:523	Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media	464:523	Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media	464:523	Batch and dynamic flow mode decolorization potential of the chitosan-alunite composite (CAC) was systematically evaluated in Acid Red 1 (AR1) and Reactive Red 2 (RR2) contaminated media.
27083375	1	55	theme	treatment	227:235	arg1	process					237:243	the wastewater treatment process	212:243	the wastewater treatment process	212:243	This study was undertaken to prepare a novel and environmentally friendly composite for the use in the wastewater treatment process.
27083375	0	56	theme	high	58:61	arg1	potential					102:110	high sorption, regeneration and application potential	58:110	high sorption, regeneration and application potential	58:110	Chitosan-alunite composite: An effective dye remover with high sorption, regeneration and application potential.
27083375	5	57	with	dyes	722:725	arg1	capacities					753:762	the maximum sorption capacities	732:762	the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively	732:806	Equilibrium sorption experiments indicated that CAC has very high sorption potential for RR2 and AR1 dyes with the maximum sorption capacities of 462.74 and 588.75 mg g(-1), respectively.
27083375	8	58	theme	anionic	1109:1115	arg1	dyes					1117:1120	anionic dyes	1109:1120	anionic dyes	1109:1120	Overall, this study suggests that CAC is a promising sorbent for the removal of anionic dyes from aqueous solutions.
27083375	6	59	theme	important	882:890	arg1	potential					837:845	reuse potential	831:845	reuse potential	831:845	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	6	59	theme	important	882:890	arg1	regeneration					814:825	Good regeneration	809:825	Good regeneration	809:825	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27083375	6	59	theme	important	882:890	arg1	advantages					892:901	other important advantages	876:901	other important advantages of this composite	876:919	Good regeneration and reuse potential in 20 consecutive cycles are other important advantages of this composite.
27112857	0	0	theme	emergent	89:96	arg1	Cr					98:99	emergent Cr(VI) spill treatment	89:119	emergent Cr(VI) spill treatment	89:119	Floatable, macroporous structured alginate sphere supporting iron nanoparticles used for emergent Cr(VI) spill treatment.
27112857	4	1	theme	effective	662:670	arg1	removal					672:678	effective removal	662:678	effective removal of Cr(VI)	662:688	This alginate-nZVI sphere exhibited good separability in effective removal of Cr(VI).
27112857	1	2	theme	chromium	146:153	arg1	accident					170:177	hexavalent chromium (Cr(VI)) spill accident	135:177	hexavalent chromium (Cr(VI)) spill accident	135:177	Treatment of hexavalent chromium (Cr(VI)) spill accident is a great challenge due to its high toxicity, sudden and extensiveness.
27112857	6	3	theme	alginate-nZVI	879:891	arg1	effective					904:912	effective	904:912	effective	904:912	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	6	3	theme	alginate-nZVI	879:891	arg1	sphere					893:898	the alginate-nZVI sphere	875:898	the alginate-nZVI sphere	875:898	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	7	4	theme	nZVI	1178:1181	arg1	properties					1164:1173	the dispersion, stabilization and separation properties	1119:1173	the dispersion, stabilization and separation properties of nZVI	1119:1181	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	6	5	from	11.0	966:969	arg1	pH					951:952	pH	951:952	pH from 3.0 to 11.0	951:969	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	6	5	from	11.0	966:969	arg1	range					942:946	a wide range	935:946	a wide range of pH from 3.0 to 11.0	935:969	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	3	6	theme	scanning	465:472	arg1	FESEM					495:499	FESEM	495:499	FESEM	495:499	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	3	6	theme	scanning	465:472	arg1	microscope					483:492	Field emission scanning electron microscope	450:492	Field emission scanning electron microscope (FESEM)	450:500	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	7	7	theme	alginate-nZVI	1019:1031	arg1	agent					1061:1065	a promising agent	1049:1065	a promising agent for an emergent Cr(VI) spill treatment	1049:1104	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	7	7	theme	alginate-nZVI	1019:1031	arg1	sphere					1033:1038	the alginate-nZVI sphere	1015:1038	the alginate-nZVI sphere	1015:1038	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	5	8	dep	efficiency	763:772	arg1	VI					751:752	VI	751:752	VI	751:752	The result from Cr(VI) removal experiment demonstrated a Cr(VI) removal efficiency of 98.2% at equilibrium time, which can be ascribed to the well dispersion of the nZVI.
27112857	5	9	dep	demonstrated	733:744	arg1	VI					710:711	VI	710:711	VI	710:711	The result from Cr(VI) removal experiment demonstrated a Cr(VI) removal efficiency of 98.2% at equilibrium time, which can be ascribed to the well dispersion of the nZVI.
27112857	6	10	theme	alginate	988:995	arg1	substrate					997:1005	alginate substrate	988:1005	alginate substrate	988:1005	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	3	11	theme	X-ray	524:528	arg1	EDS					544:546	EDS	544:546	EDS	544:546	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	3	11	theme	X-ray	524:528	arg1	spectroscopy					530:541	energy-dispersive X-ray spectroscopy	506:541	energy-dispersive X-ray spectroscopy (EDS)	506:547	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	1	12	theme	high	211:214	arg1	toxicity					216:223	its high toxicity	207:223	its high toxicity	207:223	Treatment of hexavalent chromium (Cr(VI)) spill accident is a great challenge due to its high toxicity, sudden and extensiveness.
27112857	7	13	theme	emergent	1074:1081	arg1	treatment					1096:1104	an emergent Cr(VI) spill treatment	1071:1104	an emergent Cr(VI) spill treatment	1071:1104	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	5	14	theme	nZVI	856:859	arg1	dispersion					838:847	the well dispersion	829:847	the well dispersion of the nZVI	829:859	The result from Cr(VI) removal experiment demonstrated a Cr(VI) removal efficiency of 98.2% at equilibrium time, which can be ascribed to the well dispersion of the nZVI.
27112857	3	15	theme	emission	456:463	arg1	FESEM					495:499	FESEM	495:499	FESEM	495:499	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	3	15	theme	emission	456:463	arg1	microscope					483:492	Field emission scanning electron microscope	450:492	Field emission scanning electron microscope (FESEM)	450:500	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	4	16	theme	Cr	683:684	arg1	removal					672:678	effective removal	662:678	effective removal of Cr(VI)	662:688	This alginate-nZVI sphere exhibited good separability in effective removal of Cr(VI).
27112857	7	17	dep	treatment	1096:1104	arg1	VI					1086:1087	VI	1086:1087	VI	1086:1087	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	5	18	theme	well	833:836	arg1	dispersion					838:847	the well dispersion	829:847	the well dispersion of the nZVI	829:859	The result from Cr(VI) removal experiment demonstrated a Cr(VI) removal efficiency of 98.2% at equilibrium time, which can be ascribed to the well dispersion of the nZVI.
27112857	6	19	theme	wide	937:940	arg1	range					942:946	a wide range	935:946	a wide range of pH from 3.0 to 11.0	935:969	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	6	20	theme	substrate	997:1005	arg1	merit					979:983	the merit	975:983	the merit of alginate substrate	975:1005	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	3	21	from	dispersion	568:577	arg1	composite					594:602	the composite	590:602	the composite	590:602	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	4	22	theme	alginate-nZVI	610:622	arg1	sphere					624:629	This alginate-nZVI sphere	605:629	This alginate-nZVI sphere	605:629	This alginate-nZVI sphere exhibited good separability in effective removal of Cr(VI).
27112857	6	23	from	addition	865:872	arg1	effective					904:912	effective	904:912	effective	904:912	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	6	23	from	addition	865:872	arg1	sphere					893:898	the alginate-nZVI sphere	875:898	the alginate-nZVI sphere	875:898	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	0	24	dep	Cr	98:99	arg1	VI					101:102	VI	101:102	VI	101:102	Floatable, macroporous structured alginate sphere supporting iron nanoparticles used for emergent Cr(VI) spill treatment.
27112857	0	24	dep	Cr	98:99	arg1	treatment					111:119	spill treatment	105:119	emergent Cr(VI) spill treatment	89:119	Floatable, macroporous structured alginate sphere supporting iron nanoparticles used for emergent Cr(VI) spill treatment.
27112857	7	25	theme	stabilization	1135:1147	arg1	properties					1164:1173	the dispersion, stabilization and separation properties	1119:1173	the dispersion, stabilization and separation properties of nZVI	1119:1181	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	2	26	theme	iron	404:407	arg1	sphere					441:446	the alginate-nZVI sphere	423:446	the alginate-nZVI sphere	423:446	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	2	26	theme	iron	404:407	arg1	nanoparticle					409:420	in-situ synthesized zero-valent iron nanoparticle	372:420	in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere)	372:447	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	3	27	theme	microscope	483:492	arg1	images					549:554	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images	450:554	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images	450:554	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	7	28	theme	spill	1090:1094	arg1	treatment					1096:1104	an emergent Cr(VI) spill treatment	1071:1104	an emergent Cr(VI) spill treatment	1071:1104	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	6	29	from	Cr	917:918	arg1	range					942:946	a wide range	935:946	a wide range of pH from 3.0 to 11.0	935:969	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	6	29	from	Cr	917:918	arg1	effective					904:912	effective	904:912	effective	904:912	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	6	29	from	Cr	917:918	arg1	sphere					893:898	the alginate-nZVI sphere	875:898	the alginate-nZVI sphere	875:898	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	3	30	theme	spectroscopy	530:541	arg1	images					549:554	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images	450:554	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images	450:554	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	5	31	theme	%	781:781	arg1	efficiency					763:772	a Cr(VI) removal efficiency	746:772	a Cr(VI) removal efficiency of 98.2%	746:781	The result from Cr(VI) removal experiment demonstrated a Cr(VI) removal efficiency of 98.2% at equilibrium time, which can be ascribed to the well dispersion of the nZVI.
27112857	2	32	theme	zero-valent	392:402	arg1	sphere					441:446	the alginate-nZVI sphere	423:446	the alginate-nZVI sphere	423:446	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	2	32	theme	zero-valent	392:402	arg1	nanoparticle					409:420	in-situ synthesized zero-valent iron nanoparticle	372:420	in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere)	372:447	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	4	33	dep	Cr	683:684	arg1	VI					686:687	VI	686:687	VI	686:687	This alginate-nZVI sphere exhibited good separability in effective removal of Cr(VI).
27112857	7	34	theme	dispersion	1123:1132	arg1	properties					1164:1173	the dispersion, stabilization and separation properties	1119:1173	the dispersion, stabilization and separation properties of nZVI	1119:1181	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	0	35	theme	structured	23:32	arg1	sphere					43:48	macroporous structured alginate sphere	11:48	macroporous structured alginate sphere supporting iron	11:64	Floatable, macroporous structured alginate sphere supporting iron nanoparticles used for emergent Cr(VI) spill treatment.
27112857	0	35	theme	structured	23:32	arg1	Floatable					0:8	Floatable	0:8	Floatable	0:8	Floatable, macroporous structured alginate sphere supporting iron nanoparticles used for emergent Cr(VI) spill treatment.
27112857	7	36	theme	separation	1153:1162	arg1	properties					1164:1173	the dispersion, stabilization and separation properties	1119:1173	the dispersion, stabilization and separation properties of nZVI	1119:1181	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	3	37	theme	energy-dispersive	506:522	arg1	EDS					544:546	EDS	544:546	EDS	544:546	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	3	37	theme	energy-dispersive	506:522	arg1	spectroscopy					530:541	energy-dispersive X-ray spectroscopy	506:541	energy-dispersive X-ray spectroscopy (EDS)	506:547	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	2	38	theme	in-situ	372:378	arg1	sphere					441:446	the alginate-nZVI sphere	423:446	the alginate-nZVI sphere	423:446	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	2	38	theme	in-situ	372:378	arg1	nanoparticle					409:420	in-situ synthesized zero-valent iron nanoparticle	372:420	in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere)	372:447	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	6	39	dep	Cr	917:918	arg1	VI					920:921	VI	920:921	VI	920:921	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	6	39	dep	Cr	917:918	arg1	removal					924:930	removal	924:930	Cr(VI) removal in a wide range of pH from 3.0 to 11.0	917:969	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	0	40	theme	spill	105:109	arg1	treatment					111:119	spill treatment	105:119	emergent Cr(VI) spill treatment	89:119	Floatable, macroporous structured alginate sphere supporting iron nanoparticles used for emergent Cr(VI) spill treatment.
27112857	0	41	dep	nanoparticles	66:78	arg1	used					80:83	used	80:83	nanoparticles used for emergent Cr(VI) spill treatment	66:119	Floatable, macroporous structured alginate sphere supporting iron nanoparticles used for emergent Cr(VI) spill treatment.
27112857	1	42	theme	spill	164:168	arg1	accident					170:177	hexavalent chromium (Cr(VI)) spill accident	135:177	hexavalent chromium (Cr(VI)) spill accident	135:177	Treatment of hexavalent chromium (Cr(VI)) spill accident is a great challenge due to its high toxicity, sudden and extensiveness.
27112857	2	43	theme	alginate-nZVI	427:439	arg1	sphere					441:446	the alginate-nZVI sphere	423:446	the alginate-nZVI sphere	423:446	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	2	43	theme	alginate-nZVI	427:439	arg1	nanoparticle					409:420	in-situ synthesized zero-valent iron nanoparticle	372:420	in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere)	372:447	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	5	44	theme	removal	755:761	arg1	efficiency					763:772	a Cr(VI) removal efficiency	746:772	a Cr(VI) removal efficiency of 98.2%	746:781	The result from Cr(VI) removal experiment demonstrated a Cr(VI) removal efficiency of 98.2% at equilibrium time, which can be ascribed to the well dispersion of the nZVI.
27112857	1	45	theme	accident	170:177	arg1	challenge					190:198	a great challenge	182:198	a great challenge due to its high toxicity, sudden and extensiveness	182:249	Treatment of hexavalent chromium (Cr(VI)) spill accident is a great challenge due to its high toxicity, sudden and extensiveness.
27112857	1	45	theme	accident	170:177	arg1	Treatment					122:130	Treatment	122:130	Treatment of hexavalent chromium (Cr(VI)) spill accident	122:177	Treatment of hexavalent chromium (Cr(VI)) spill accident is a great challenge due to its high toxicity, sudden and extensiveness.
27112857	0	46	theme	alginate	34:41	arg1	sphere					43:48	macroporous structured alginate sphere	11:48	macroporous structured alginate sphere supporting iron	11:64	Floatable, macroporous structured alginate sphere supporting iron nanoparticles used for emergent Cr(VI) spill treatment.
27112857	0	46	theme	alginate	34:41	arg1	Floatable					0:8	Floatable	0:8	Floatable	0:8	Floatable, macroporous structured alginate sphere supporting iron nanoparticles used for emergent Cr(VI) spill treatment.
27112857	2	47	theme	synthesized	380:390	arg1	sphere					441:446	the alginate-nZVI sphere	423:446	the alginate-nZVI sphere	423:446	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	2	47	theme	synthesized	380:390	arg1	nanoparticle					409:420	in-situ synthesized zero-valent iron nanoparticle	372:420	in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere)	372:447	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	2	48	theme	alginate	345:352	arg1	sphere					354:359	a hierarchical, ordered and macroporous structured alginate sphere	294:359	a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere)	294:447	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	5	49	theme	removal	714:720	arg1	experiment					722:731	removal experiment	714:731	removal experiment	714:731	The result from Cr(VI) removal experiment demonstrated a Cr(VI) removal efficiency of 98.2% at equilibrium time, which can be ascribed to the well dispersion of the nZVI.
27112857	5	50	theme	equilibrium	786:796	arg1	time					798:801	equilibrium time	786:801	equilibrium time	786:801	The result from Cr(VI) removal experiment demonstrated a Cr(VI) removal efficiency of 98.2% at equilibrium time, which can be ascribed to the well dispersion of the nZVI.
27112857	1	51	dep	Cr	156:157	arg1	VI					159:160	VI	159:160	VI	159:160	Treatment of hexavalent chromium (Cr(VI)) spill accident is a great challenge due to its high toxicity, sudden and extensiveness.
27112857	5	52	from	Cr	707:708	arg1	result					695:700	The result	691:700	The result from Cr	691:708	The result from Cr(VI) removal experiment demonstrated a Cr(VI) removal efficiency of 98.2% at equilibrium time, which can be ascribed to the well dispersion of the nZVI.
27112857	5	53	theme	Cr	748:749	arg1	efficiency					763:772	a Cr(VI) removal efficiency	746:772	a Cr(VI) removal efficiency of 98.2%	746:781	The result from Cr(VI) removal experiment demonstrated a Cr(VI) removal efficiency of 98.2% at equilibrium time, which can be ascribed to the well dispersion of the nZVI.
27112857	3	54	theme	Field	450:454	arg1	FESEM					495:499	FESEM	495:499	FESEM	495:499	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	3	54	theme	Field	450:454	arg1	microscope					483:492	Field emission scanning electron microscope	450:492	Field emission scanning electron microscope (FESEM)	450:500	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	6	55	dep	11.0	966:969	arg1	to					963:964	to	963:964	to	963:964	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	4	56	theme	good	641:644	arg1	separability					646:657	good separability	641:657	good separability	641:657	This alginate-nZVI sphere exhibited good separability in effective removal of Cr(VI).
27112857	7	57	theme	Cr	1083:1084	arg1	treatment					1096:1104	an emergent Cr(VI) spill treatment	1071:1104	an emergent Cr(VI) spill treatment	1071:1104	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	2	58	dep	hierarchical	296:307	arg1	ordered					310:316	ordered	310:316	ordered	310:316	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	2	58	dep	hierarchical	296:307	arg1	structured					334:343	structured	334:343	structured	334:343	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
27112857	3	59	theme	nZVI	582:585	arg1	dispersion					568:577	dispersion	568:577	dispersion of nZVI on the composite	568:602	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	1	60	theme	hexavalent	135:144	arg1	accident					170:177	hexavalent chromium (Cr(VI)) spill accident	135:177	hexavalent chromium (Cr(VI)) spill accident	135:177	Treatment of hexavalent chromium (Cr(VI)) spill accident is a great challenge due to its high toxicity, sudden and extensiveness.
27112857	1	61	theme	great	184:188	arg1	challenge					190:198	a great challenge	182:198	a great challenge due to its high toxicity, sudden and extensiveness	182:249	Treatment of hexavalent chromium (Cr(VI)) spill accident is a great challenge due to its high toxicity, sudden and extensiveness.
27112857	1	61	theme	great	184:188	arg1	Treatment					122:130	Treatment	122:130	Treatment of hexavalent chromium (Cr(VI)) spill accident	122:177	Treatment of hexavalent chromium (Cr(VI)) spill accident is a great challenge due to its high toxicity, sudden and extensiveness.
27112857	6	62	theme	pH	951:952	arg1	range					942:946	a wide range	935:946	a wide range of pH from 3.0 to 11.0	935:969	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	6	63	from	effective	904:912	arg1	addition					865:872	addition	865:872	addition	865:872	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	6	63	from	effective	904:912	arg1	Cr					917:918	Cr(VI) removal in a wide range of pH from 3.0 to 11.0	917:969	Cr(VI) removal in a wide range of pH from 3.0 to 11.0	917:969	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	6	64	from	range	942:946	arg1	11.0					966:969	11.0	966:969	11.0	966:969	In addition, the alginate-nZVI sphere was effective in Cr(VI) removal in a wide range of pH from 3.0 to 11.0, by the merit of alginate substrate.
27112857	3	65	theme	electron	474:481	arg1	FESEM					495:499	FESEM	495:499	FESEM	495:499	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	3	65	theme	electron	474:481	arg1	microscope					483:492	Field emission scanning electron microscope	450:492	Field emission scanning electron microscope (FESEM)	450:500	Field emission scanning electron microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDS) images showed well dispersion of nZVI on the composite.
27112857	7	66	theme	promising	1051:1059	arg1	agent					1061:1065	a promising agent	1049:1065	a promising agent for an emergent Cr(VI) spill treatment	1049:1104	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	7	66	theme	promising	1051:1059	arg1	sphere					1033:1038	the alginate-nZVI sphere	1015:1038	the alginate-nZVI sphere	1015:1038	Hence, the alginate-nZVI sphere might be a promising agent for an emergent Cr(VI) spill treatment by enhancing the dispersion, stabilization and separation properties of nZVI.
27112857	0	67	theme	macroporous	11:21	arg1	sphere					43:48	macroporous structured alginate sphere	11:48	macroporous structured alginate sphere supporting iron	11:64	Floatable, macroporous structured alginate sphere supporting iron nanoparticles used for emergent Cr(VI) spill treatment.
27112857	0	67	theme	macroporous	11:21	arg1	Floatable					0:8	Floatable	0:8	Floatable	0:8	Floatable, macroporous structured alginate sphere supporting iron nanoparticles used for emergent Cr(VI) spill treatment.
27112857	2	68	theme	hierarchical	296:307	arg1	sphere					354:359	a hierarchical, ordered and macroporous structured alginate sphere	294:359	a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere)	294:447	In this study, we designed and fabricated a hierarchical, ordered and macroporous structured alginate sphere to support in-situ synthesized zero-valent iron nanoparticle (the alginate-nZVI sphere).
24730244	0	0	theme	antitumor	92:100	arg1	activity					102:109	in vitro antitumor activity	83:109	in vitro antitumor activity against SMMC-7721 cells	83:133	Nanofibrous mats layer-by-layer assembled by HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells.
24730244	1	1	theme	charged	333:339	arg1	nanocomposites					351:364	the positively charged HTCC-OREC nanocomposites	318:364	the positively charged HTCC-OREC nanocomposites	318:364	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	2	2	theme	average	616:622	arg1	diameter					630:637	the average fiber diameter	612:637	the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously	612:683	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	5	3	theme	structured	1253:1262	arg1	mats					1276:1279	LBL structured nanofibrous mats	1249:1279	LBL structured nanofibrous mats	1249:1279	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	1	4	theme	chitosan	261:268	arg1	chloride					270:277	N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride	218:277	N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC)	218:284	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	1	4	theme	chitosan	261:268	arg1	HTCC					280:283	HTCC	280:283	HTCC	280:283	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	4	5	from	effect	1087:1092	arg1	cells					1124:1128	human Chang liver (CCL-13) cells	1097:1128	human Chang liver (CCL-13) cells	1097:1128	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	1	6	theme	HTCC-OREC	341:349	arg1	nanocomposites					351:364	the positively charged HTCC-OREC nanocomposites	318:364	the positively charged HTCC-OREC nanocomposites	318:364	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	5	7	theme	nanofibrous	1264:1274	arg1	mats					1276:1279	LBL structured nanofibrous mats	1249:1279	LBL structured nanofibrous mats	1249:1279	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	5	8	theme	Fluorescence	1194:1205	arg1	Microscope					1207:1216	FE-SEM and Inverted Fluorescence Microscope	1174:1216	FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats	1174:1279	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	2	9	theme	Field	540:544	arg1	FE-SEM					585:590	FE-SEM	585:590	FE-SEM	585:590	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	2	9	theme	Field	540:544	arg1	Microscopy					573:582	Field Emission Scanning Electron Microscopy	540:582	Field Emission Scanning Electron Microscopy (FE-SEM) images	540:598	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	1	10	theme	nanocomposites	351:364	arg1	immobilization					300:313	then the immobilization	291:313	then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats	291:443	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	1	11	used	used	165:168	arg2	OREC					155:158	OREC	155:158	OREC	155:158	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	1	11	used	used	165:168	arg2	rectorite					144:152	Organic rectorite	136:152	Organic rectorite (OREC)	136:159	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	3	12	dep	spectroscopy	745:756	arg1	results					792:798	results	792:798	results	792:798	Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template.
24730244	1	13	from	immobilization	300:313	arg1	mats					440:443	cellulose nanofibrous mats	418:443	cellulose nanofibrous mats	418:443	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	4	14	theme	prepared	944:951	arg1	mats					965:968	the prepared nanofibrous mats	940:968	the prepared nanofibrous mats	940:968	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	6	15	theme	developed	1354:1362	arg1	approach					1364:1371	The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness	1350:1452	The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness	1350:1452	The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness may also be utilized to tumor therapy.
24730244	2	16	dep	608	707:709	arg1	to					704:705	to	704:705	to	704:705	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	0	17	theme	SMMC-7721	119:127	arg1	cells					129:133	SMMC-7721 cells	119:133	SMMC-7721 cells	119:133	Nanofibrous mats layer-by-layer assembled by HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells.
24730244	5	18	theme	Inverted	1185:1192	arg1	Microscope					1207:1216	FE-SEM and Inverted Fluorescence Microscope	1174:1216	FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats	1174:1279	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	1	19	theme	layer-by-layer	467:480	arg1	technique					488:496	layer-by-layer (LBL) technique	467:496	layer-by-layer (LBL) technique	467:496	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	4	20	theme	hepatocellular	1021:1034	arg1	carcinoma					1036:1044	human hepatocellular carcinoma	1015:1044	human hepatocellular carcinoma cells (SMMC-7721)	1015:1062	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	3	21	theme	successful	866:875	arg1	immobilization					877:890	their successful immobilization	860:890	their successful immobilization on cellulose template	860:912	Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template.
24730244	4	22	theme	carcinoma	1036:1044	arg1	SMMC-7721					1053:1061	SMMC-7721	1053:1061	SMMC-7721	1053:1061	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	4	22	theme	carcinoma	1036:1044	arg1	cells					1046:1050	human hepatocellular carcinoma cells	1015:1050	human hepatocellular carcinoma cells (SMMC-7721)	1015:1062	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	1	23	theme	charged	385:391	arg1	ALG					410:412	ALG	410:412	ALG	410:412	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	1	23	theme	charged	385:391	arg1	alginate					400:407	the negatively charged sodium alginate	370:407	the negatively charged sodium alginate (ALG)	370:413	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	2	24	theme	diameter	505:512	arg1	distribution					514:525	Fiber diameter distribution	499:525	Fiber diameter distribution	499:525	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	2	25	theme	Scanning	555:562	arg1	FE-SEM					585:590	FE-SEM	585:590	FE-SEM	585:590	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	2	25	theme	Scanning	555:562	arg1	Microscopy					573:582	Field Emission Scanning Electron Microscopy	540:582	Field Emission Scanning Electron Microscopy (FE-SEM) images	540:598	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	3	26	theme	photoelectron	731:743	arg1	XPS					759:761	XPS	759:761	XPS	759:761	Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template.
24730244	3	26	theme	photoelectron	731:743	arg1	spectroscopy					745:756	X-ray photoelectron spectroscopy	725:756	X-ray photoelectron spectroscopy (XPS)	725:762	Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template.
24730244	2	27	theme	fiber	624:628	arg1	diameter					630:637	the average fiber diameter	612:637	the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously	612:683	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	1	28	theme	LBL	483:485	arg1	technique					488:496	layer-by-layer (LBL) technique	467:496	layer-by-layer (LBL) technique	467:496	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	3	29	from	interaction	822:832	arg1	template					905:912	cellulose template	895:912	cellulose template	895:912	Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template.
24730244	4	30	theme	Chang	1103:1107	arg1	cells					1124:1128	human Chang liver (CCL-13) cells	1097:1128	human Chang liver (CCL-13) cells	1097:1128	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	1	31	theme	intercalated	185:196	arg1	nanocomposites					198:211	the intercalated nanocomposites	181:211	the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC)	181:284	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	2	32	theme	films	661:665	arg1	diameter					630:637	the average fiber diameter	612:637	the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously	612:683	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	2	33	theme	Microscopy	573:582	arg1	images					593:598	Field Emission Scanning Electron Microscopy (FE-SEM) images	540:598	Field Emission Scanning Electron Microscopy (FE-SEM) images	540:598	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	0	34	dep	in	83:84	arg1	vitro					86:90	vitro	86:90	vitro	86:90	Nanofibrous mats layer-by-layer assembled by HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells.
24730244	4	35	theme	human	1097:1101	arg1	cells					1124:1128	human Chang liver (CCL-13) cells	1097:1128	human Chang liver (CCL-13) cells	1097:1128	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	6	36	theme	controllable	1431:1442	arg1	thickness					1444:1452	controllable thickness	1431:1452	controllable thickness	1431:1452	The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness may also be utilized to tumor therapy.
24730244	5	37	theme	FE-SEM	1174:1179	arg1	Microscope					1207:1216	FE-SEM and Inverted Fluorescence Microscope	1174:1216	FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats	1174:1279	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	2	38	theme	Electron	564:571	arg1	FE-SEM					585:590	FE-SEM	585:590	FE-SEM	585:590	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	2	38	theme	Electron	564:571	arg1	Microscopy					573:582	Field Emission Scanning Electron Microscopy	540:582	Field Emission Scanning Electron Microscopy (FE-SEM) images	540:598	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	1	39	theme	sodium	393:398	arg1	ALG					410:412	ALG	410:412	ALG	410:412	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	1	39	theme	sodium	393:398	arg1	alginate					400:407	the negatively charged sodium alginate	370:407	the negatively charged sodium alginate (ALG)	370:413	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	5	40	theme	samples	1341:1347	arg1	activity					1320:1327	the significant antitumor activity	1294:1327	the significant antitumor activity of prepared samples	1294:1347	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	5	41	theme	prepared	1332:1339	arg1	samples					1341:1347	prepared samples	1332:1347	prepared samples	1332:1347	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	0	42	theme	HTCC/layered	45:56	arg1	composites					67:76	HTCC/layered silicate composites	45:76	HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells	45:133	Nanofibrous mats layer-by-layer assembled by HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells.
24730244	3	43	theme	cellulose	895:903	arg1	template					905:912	cellulose template	895:912	cellulose template	895:912	Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template.
24730244	1	44	with	nanocomposites	198:211	arg1	chloride					270:277	N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride	218:277	N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC)	218:284	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	1	44	with	nanocomposites	198:211	arg1	HTCC					280:283	HTCC	280:283	HTCC	280:283	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	1	45	theme	alginate	400:407	arg1	immobilization					300:313	then the immobilization	291:313	then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats	291:443	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	4	46	theme	MTT	915:917	arg1	assay					919:923	MTT assay	915:923	MTT assay	915:923	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	4	47	theme	liver	1109:1113	arg1	cells					1124:1128	human Chang liver (CCL-13) cells	1097:1128	human Chang liver (CCL-13) cells	1097:1128	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	4	48	theme	inhibitory	987:996	arg1	activity					998:1005	strong inhibitory activity	980:1005	strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721)	980:1062	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	2	49	theme	Emission	546:553	arg1	FE-SEM					585:590	FE-SEM	585:590	FE-SEM	585:590	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	2	49	theme	Emission	546:553	arg1	Microscopy					573:582	Field Emission Scanning Electron Microscopy	540:582	Field Emission Scanning Electron Microscopy (FE-SEM) images	540:598	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	3	50	theme	X-ray	768:772	arg1	XRD					787:789	XRD	787:789	XRD	787:789	Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template.
24730244	3	50	theme	X-ray	768:772	arg1	diffraction					774:784	X-ray diffraction	768:784	X-ray diffraction (XRD)	768:790	Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template.
24730244	6	51	with	nanofibers	1415:1424	arg1	thickness					1444:1452	controllable thickness	1431:1452	controllable thickness	1431:1452	The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness may also be utilized to tumor therapy.
24730244	4	52	theme	human	1015:1019	arg1	carcinoma					1036:1044	human hepatocellular carcinoma	1015:1044	human hepatocellular carcinoma cells (SMMC-7721)	1015:1062	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	4	53	theme	CCL-13	1116:1121	arg1	cells					1124:1128	human Chang liver (CCL-13) cells	1097:1128	human Chang liver (CCL-13) cells	1097:1128	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	4	54	theme	nanofibrous	953:963	arg1	mats					965:968	the prepared nanofibrous mats	940:968	the prepared nanofibrous mats	940:968	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	3	55	theme	X-ray	725:729	arg1	XPS					759:761	XPS	759:761	XPS	759:761	Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template.
24730244	3	55	theme	X-ray	725:729	arg1	spectroscopy					745:756	X-ray photoelectron spectroscopy	725:756	X-ray photoelectron spectroscopy (XPS)	725:762	Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template.
24730244	6	56	theme	tumor	1478:1482	arg1	therapy					1484:1490	tumor therapy	1478:1490	tumor therapy	1478:1490	The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness may also be utilized to tumor therapy.
24730244	0	57	theme	silicate	58:65	arg1	composites					67:76	HTCC/layered silicate composites	45:76	HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells	45:133	Nanofibrous mats layer-by-layer assembled by HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells.
24730244	5	58	from	Microscope	1207:1216	arg1	results					1161:1167	the experimental results	1144:1167	the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats	1144:1279	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	6	59	used	utilized	1466:1473	arg2	approach					1364:1371	The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness	1350:1452	The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness	1350:1452	The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness may also be utilized to tumor therapy.
24730244	1	60	theme	Organic	136:142	arg1	rectorite					144:152	Organic rectorite	136:152	Organic rectorite (OREC)	136:159	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	1	60	theme	Organic	136:142	arg1	OREC					155:158	OREC	155:158	OREC	155:158	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	4	61	theme	cytotoxic	1077:1085	arg1	effect					1087:1092	a little cytotoxic effect	1068:1092	a little cytotoxic effect on human Chang liver (CCL-13) cells	1068:1128	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	5	62	theme	experimental	1148:1159	arg1	results					1161:1167	the experimental results	1144:1167	the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats	1144:1279	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	4	63	theme	little	1070:1075	arg1	effect					1087:1092	a little cytotoxic effect	1068:1092	a little cytotoxic effect on human Chang liver (CCL-13) cells	1068:1128	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	5	64	theme	SMMC-7721	1221:1229	arg1	cells					1231:1235	SMMC-7721 cells	1221:1235	SMMC-7721 cells cultured on LBL structured nanofibrous mats	1221:1279	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	6	65	theme	polymer	1407:1413	arg1	nanofibers					1415:1424	polymer nanofibers	1407:1424	polymer nanofibers with controllable thickness	1407:1452	The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness may also be utilized to tumor therapy.
24730244	1	66	theme	cellulose	418:426	arg1	mats					440:443	cellulose nanofibrous mats	418:443	cellulose nanofibrous mats	418:443	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	3	67	from	immobilization	877:890	arg1	template					905:912	cellulose template	895:912	cellulose template	895:912	Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template.
24730244	0	68	with	composites	67:76	arg1	activity					102:109	in vitro antitumor activity	83:109	in vitro antitumor activity against SMMC-7721 cells	83:133	Nanofibrous mats layer-by-layer assembled by HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells.
24730244	5	69	theme	significant	1298:1308	arg1	activity					1320:1327	the significant antitumor activity	1294:1327	the significant antitumor activity of prepared samples	1294:1347	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	5	70	theme	cells	1231:1235	arg1	Microscope					1207:1216	FE-SEM and Inverted Fluorescence Microscope	1174:1216	FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats	1174:1279	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	0	71	theme	in	83:84	arg1	activity					102:109	in vitro antitumor activity	83:109	in vitro antitumor activity against SMMC-7721 cells	83:133	Nanofibrous mats layer-by-layer assembled by HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells.
24730244	1	72	theme	nanofibrous	428:438	arg1	mats					440:443	cellulose nanofibrous mats	418:443	cellulose nanofibrous mats	418:443	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	0	73	dep	mats	12:15	arg1	assembled					32:40	assembled	32:40	mats layer-by-layer assembled by HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells	12:133	Nanofibrous mats layer-by-layer assembled by HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells.
24730244	5	74	theme	antitumor	1310:1318	arg1	activity					1320:1327	the significant antitumor activity	1294:1327	the significant antitumor activity of prepared samples	1294:1347	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	1	75	theme	propyl-3-trimethyl	233:250	arg1	chloride					270:277	N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride	218:277	N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC)	218:284	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	1	75	theme	propyl-3-trimethyl	233:250	arg1	HTCC					280:283	HTCC	280:283	HTCC	280:283	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	2	76	theme	Fiber	499:503	arg1	distribution					514:525	Fiber diameter distribution	499:525	Fiber diameter distribution	499:525	Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm.
24730244	4	77	theme	strong	980:985	arg1	activity					998:1005	strong inhibitory activity	980:1005	strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721)	980:1062	MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells.
24730244	5	78	theme	LBL	1249:1251	arg1	mats					1276:1279	LBL structured nanofibrous mats	1249:1279	LBL structured nanofibrous mats	1249:1279	Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples.
24730244	1	79	theme	ammonium	252:259	arg1	chloride					270:277	N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride	218:277	N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC)	218:284	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
24730244	1	79	theme	ammonium	252:259	arg1	HTCC					280:283	HTCC	280:283	HTCC	280:283	Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique.
26213074	5	0	theme	adulteration	769:780	arg1	identification					782:795	adulteration identification	769:795	adulteration identification	769:795	This strategy, validated by manual mixtures, optimized both the reliability and authority of adulteration identification.
26213074	3	1	from	identification	420:433	arg1	coffee					445:450	ground coffee	438:450	ground coffee	438:450	In this work, a novel strategy for adulteration identification in ground coffee was developed based on UPLC-HRMS oligosaccharide profiling.
26213074	1	2	theme	valuable	149:156	arg1	beverages					158:166	the most common and most valuable beverages	124:166	the most common and most valuable beverages	124:166	Coffee is one of the most common and most valuable beverages.
26213074	0	3	theme	oligosaccharide	80:94	arg1	profiling					96:104	UPLC-HRMS oligosaccharide profiling	70:104	UPLC-HRMS oligosaccharide profiling	70:104	Novel identification strategy for ground coffee adulteration based on UPLC-HRMS oligosaccharide profiling.
26213074	6	4	theme	in	852:853	arg1	amounts					855:861	ground coffee in amounts	838:861	ground coffee in amounts as low as 5%	838:874	Rice and soybean adulterants present in ground coffee in amounts as low as 5% were identified and evaluated.
26213074	6	5	theme	ground	838:843	arg1	amounts					855:861	ground coffee in amounts	838:861	ground coffee in amounts as low as 5%	838:874	Rice and soybean adulterants present in ground coffee in amounts as low as 5% were identified and evaluated.
26213074	1	6	theme	beverages	158:166	arg1	beverages					158:166	the most common and most valuable beverages	124:166	the most common and most valuable beverages	124:166	Coffee is one of the most common and most valuable beverages.
26213074	1	6	theme	beverages	158:166	arg1	one					117:119	one	117:119	one	117:119	Coffee is one of the most common and most valuable beverages.
26213074	6	7	theme	present	827:833	arg1	adulterants					815:825	Rice and soybean adulterants	798:825	Rice and soybean adulterants present in ground coffee in amounts as low as 5%	798:874	Rice and soybean adulterants present in ground coffee in amounts as low as 5% were identified and evaluated.
26213074	3	8	theme	oligosaccharide	485:499	arg1	profiling					501:509	UPLC-HRMS oligosaccharide profiling	475:509	UPLC-HRMS oligosaccharide profiling	475:509	In this work, a novel strategy for adulteration identification in ground coffee was developed based on UPLC-HRMS oligosaccharide profiling.
26213074	3	9	theme	adulteration	407:418	arg1	identification					420:433	adulteration identification	407:433	adulteration identification in ground coffee	407:450	In this work, a novel strategy for adulteration identification in ground coffee was developed based on UPLC-HRMS oligosaccharide profiling.
26213074	6	10	attach	present	827:833	arg1	amounts					855:861	ground coffee in amounts	838:861	ground coffee in amounts as low as 5%	838:874	Rice and soybean adulterants present in ground coffee in amounts as low as 5% were identified and evaluated.
26213074	6	10	attach	present	827:833	arg2	adulterants					815:825	Rice and soybean adulterants	798:825	Rice and soybean adulterants present in ground coffee in amounts as low as 5%	798:874	Rice and soybean adulterants present in ground coffee in amounts as low as 5% were identified and evaluated.
26213074	6	11	theme	soybean	807:813	arg1	adulterants					815:825	Rice and soybean adulterants	798:825	Rice and soybean adulterants present in ground coffee in amounts as low as 5%	798:874	Rice and soybean adulterants present in ground coffee in amounts as low as 5% were identified and evaluated.
26213074	2	12	theme	financial	262:270	arg1	reasons					272:278	financial reasons	262:278	financial reasons	262:278	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	4	13	theme	ground	661:666	arg1	coffee					668:673	ground coffee	661:673	ground coffee	661:673	Along with integrated statistical analysis, 17 oligosaccharide composition were identified as markers for the identification of soybeans and rice in ground coffee.
26213074	0	14	theme	identification	6:19	arg1	strategy					21:28	Novel identification strategy	0:28	Novel identification strategy for ground coffee adulteration	0:59	Novel identification strategy for ground coffee adulteration based on UPLC-HRMS oligosaccharide profiling.
26213074	5	15	theme	identification	782:795	arg1	reliability					740:750	reliability	740:750	reliability	740:750	This strategy, validated by manual mixtures, optimized both the reliability and authority of adulteration identification.
26213074	5	15	theme	identification	782:795	arg1	authority					756:764	authority	756:764	authority	756:764	This strategy, validated by manual mixtures, optimized both the reliability and authority of adulteration identification.
26213074	2	16	theme	serious	304:310	arg1	threat					312:317	the most serious threat	295:317	the most serious threat to the sustainable development of the coffee market	295:369	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	2	16	theme	serious	304:310	arg1	adulteration					235:246	the adulteration	231:246	the adulteration of coffee for financial reasons	231:278	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	0	17	theme	Novel	0:4	arg1	strategy					21:28	Novel identification strategy	0:28	Novel identification strategy for ground coffee adulteration	0:59	Novel identification strategy for ground coffee adulteration based on UPLC-HRMS oligosaccharide profiling.
26213074	6	18	theme	coffee	845:850	arg1	amounts					855:861	ground coffee in amounts	838:861	ground coffee in amounts as low as 5%	838:874	Rice and soybean adulterants present in ground coffee in amounts as low as 5% were identified and evaluated.
26213074	6	19	theme	Rice	798:801	arg1	adulterants					815:825	Rice and soybean adulterants	798:825	Rice and soybean adulterants present in ground coffee in amounts as low as 5%	798:874	Rice and soybean adulterants present in ground coffee in amounts as low as 5% were identified and evaluated.
26213074	2	20	theme	market	364:369	arg1	development					338:348	the sustainable development	322:348	the sustainable development of the coffee market	322:369	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	5	21	theme	manual	704:709	arg1	mixtures					711:718	manual mixtures	704:718	manual mixtures	704:718	This strategy, validated by manual mixtures, optimized both the reliability and authority of adulteration identification.
26213074	4	22	from	rice	653:656	arg1	coffee					668:673	ground coffee	661:673	ground coffee	661:673	Along with integrated statistical analysis, 17 oligosaccharide composition were identified as markers for the identification of soybeans and rice in ground coffee.
26213074	2	23	theme	International	182:194	arg1	reports					222:228	International Coffee Organization (ICO) reports	182:228	International Coffee Organization (ICO) reports	182:228	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	4	24	theme	soybeans	640:647	arg1	rice					653:656	rice	653:656	rice in ground coffee	653:673	Along with integrated statistical analysis, 17 oligosaccharide composition were identified as markers for the identification of soybeans and rice in ground coffee.
26213074	4	24	theme	soybeans	640:647	arg1	identification					622:635	the identification	618:635	the identification of soybeans	618:647	Along with integrated statistical analysis, 17 oligosaccharide composition were identified as markers for the identification of soybeans and rice in ground coffee.
26213074	0	25	theme	coffee	41:46	arg1	adulteration					48:59	ground coffee adulteration	34:59	ground coffee adulteration	34:59	Novel identification strategy for ground coffee adulteration based on UPLC-HRMS oligosaccharide profiling.
26213074	4	26	theme	oligosaccharide	559:573	arg1	markers					606:612	markers	606:612	markers for the identification of soybeans and rice in ground coffee	606:673	Along with integrated statistical analysis, 17 oligosaccharide composition were identified as markers for the identification of soybeans and rice in ground coffee.
26213074	4	26	theme	oligosaccharide	559:573	arg1	composition					575:585	17 oligosaccharide composition	556:585	17 oligosaccharide composition	556:585	Along with integrated statistical analysis, 17 oligosaccharide composition were identified as markers for the identification of soybeans and rice in ground coffee.
26213074	0	27	theme	ground	34:39	arg1	adulteration					48:59	ground coffee adulteration	34:59	ground coffee adulteration	34:59	Novel identification strategy for ground coffee adulteration based on UPLC-HRMS oligosaccharide profiling.
26213074	4	28	theme	integrated	523:532	arg1	analysis					546:553	integrated statistical analysis	523:553	integrated statistical analysis	523:553	Along with integrated statistical analysis, 17 oligosaccharide composition were identified as markers for the identification of soybeans and rice in ground coffee.
26213074	3	29	theme	novel	388:392	arg1	strategy					394:401	a novel strategy	386:401	a novel strategy for adulteration identification in ground coffee	386:450	In this work, a novel strategy for adulteration identification in ground coffee was developed based on UPLC-HRMS oligosaccharide profiling.
26213074	3	30	theme	ground	438:443	arg1	coffee					445:450	ground coffee	438:450	ground coffee	438:450	In this work, a novel strategy for adulteration identification in ground coffee was developed based on UPLC-HRMS oligosaccharide profiling.
26213074	5	31	dep	reliability	740:750	arg1	the					736:738	the	736:738	the	736:738	This strategy, validated by manual mixtures, optimized both the reliability and authority of adulteration identification.
26213074	6	32	theme	low	866:868	arg1	amounts					855:861	ground coffee in amounts	838:861	ground coffee in amounts as low as 5%	838:874	Rice and soybean adulterants present in ground coffee in amounts as low as 5% were identified and evaluated.
26213074	2	33	theme	sustainable	326:336	arg1	development					338:348	the sustainable development	322:348	the sustainable development of the coffee market	322:369	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	6	34	from	amounts	855:861	arg1	present					827:833	present	827:833	present	827:833	Rice and soybean adulterants present in ground coffee in amounts as low as 5% were identified and evaluated.
26213074	2	35	theme	ICO	217:219	arg1	reports					222:228	International Coffee Organization (ICO) reports	182:228	International Coffee Organization (ICO) reports	182:228	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	6	36	from	present	827:833	arg1	amounts					855:861	ground coffee in amounts	838:861	ground coffee in amounts as low as 5%	838:874	Rice and soybean adulterants present in ground coffee in amounts as low as 5% were identified and evaluated.
26213074	3	37	theme	UPLC-HRMS	475:483	arg1	profiling					501:509	UPLC-HRMS oligosaccharide profiling	475:509	UPLC-HRMS oligosaccharide profiling	475:509	In this work, a novel strategy for adulteration identification in ground coffee was developed based on UPLC-HRMS oligosaccharide profiling.
26213074	7	38	theme	commercial	912:921	arg1	coffees					930:936	Some commercial ground coffees	907:936	Some commercial ground coffees	907:936	Some commercial ground coffees were also successfully tested using this strategy.
26213074	4	39	theme	statistical	534:544	arg1	analysis					546:553	integrated statistical analysis	523:553	integrated statistical analysis	523:553	Along with integrated statistical analysis, 17 oligosaccharide composition were identified as markers for the identification of soybeans and rice in ground coffee.
26213074	7	40	theme	ground	923:928	arg1	coffees					930:936	Some commercial ground coffees	907:936	Some commercial ground coffees	907:936	Some commercial ground coffees were also successfully tested using this strategy.
26213074	2	41	theme	Organization	203:214	arg1	reports					222:228	International Coffee Organization (ICO) reports	182:228	International Coffee Organization (ICO) reports	182:228	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	2	42	theme	coffee	251:256	arg1	threat					312:317	the most serious threat	295:317	the most serious threat to the sustainable development of the coffee market	295:369	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	2	42	theme	coffee	251:256	arg1	adulteration					235:246	the adulteration	231:246	the adulteration of coffee for financial reasons	231:278	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	1	43	theme	common	133:138	arg1	beverages					158:166	the most common and most valuable beverages	124:166	the most common and most valuable beverages	124:166	Coffee is one of the most common and most valuable beverages.
26213074	0	44	theme	UPLC-HRMS	70:78	arg1	profiling					96:104	UPLC-HRMS oligosaccharide profiling	70:104	UPLC-HRMS oligosaccharide profiling	70:104	Novel identification strategy for ground coffee adulteration based on UPLC-HRMS oligosaccharide profiling.
26213074	2	45	theme	Coffee	196:201	arg1	reports					222:228	International Coffee Organization (ICO) reports	182:228	International Coffee Organization (ICO) reports	182:228	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	2	46	theme	coffee	357:362	arg1	market					364:369	the coffee market	353:369	the coffee market	353:369	According to International Coffee Organization (ICO) reports, the adulteration of coffee for financial reasons is regarded as the most serious threat to the sustainable development of the coffee market.
26213074	4	47	from	identification	622:635	arg1	coffee					668:673	ground coffee	661:673	ground coffee	661:673	Along with integrated statistical analysis, 17 oligosaccharide composition were identified as markers for the identification of soybeans and rice in ground coffee.
23893795	1	0	theme	intermediate	362:373	arg1	enantiomers					375:385	three intermediate enantiomers	356:385	its three intermediate enantiomers (intermediates II-IV)	352:407	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	1	0	theme	intermediate	362:373	arg1	intermediates					388:400	intermediates II-IV	388:406	intermediates II-IV	388:406	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	4	1	theme	2.0	910:912	arg1	mmol/L					914:919	mmol/L	914:919	mmol/L	914:919	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	1	2	theme	new	177:179	arg1	CE					208:209	CE	208:209	CE	208:209	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	1	2	theme	new	177:179	arg1	electrophoresis					191:205	A new capillary electrophoresis	175:205	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector	175:281	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	4	3	theme	20	932:933	arg1	mmol/L					935:940	mmol/L	935:940	mmol/L	935:940	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	0	4	theme	capillary	80:88	arg1	electrophoresis					90:104	capillary electrophoresis	80:104	capillary electrophoresis	80:104	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.
23893795	1	5	theme	enantiomers	375:385	arg1	separation					323:332	the enantiomeric separation	306:332	the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV)	306:407	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	2	6	theme	applied	681:687	arg1	voltage					689:695	the applied voltage	677:695	the applied voltage	677:695	The primary factors affecting the separation efficiency, which include the chiral selector and its concentration, the buffer pH and composition, the organic modifiers used, and the applied voltage, were optimized.
23893795	1	7	theme	capillary	181:189	arg1	CE					208:209	CE	208:209	CE	208:209	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	1	7	theme	capillary	181:189	arg1	electrophoresis					191:205	A new capillary electrophoresis	175:205	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector	175:281	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	0	8	theme	quantitative	107:118	arg1	analysis					120:127	quantitative analysis	107:127	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.	0:173	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.
23893795	1	9	theme	meptazinol	477:486	arg1	determination					460:472	quantitative determination	447:472	quantitative determination of meptazinol in tablets	447:497	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	5	10	theme	pharmaceutical	1252:1265	arg1	formulations					1267:1278	pharmaceutical formulations	1252:1278	pharmaceutical formulations	1252:1278	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	5	11	from	terms	1080:1084	arg1	validation					1052:1061	validation	1052:1061	validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity	1052:1176	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	4	12	theme	mmol/L	914:919	arg1	CM-β-CD					921:927	2.0 mmol/L CM-β-CD	910:927	2.0 mmol/L CM-β-CD	910:927	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	4	13	theme	6.00	976:979	arg1	pH					970:971	a pH	968:971	a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile	968:1043	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	5	14	theme	meptazinol	1238:1247	arg1	quantitation					1222:1233	the quantitation	1218:1233	the quantitation of meptazinol in pharmaceutical formulations	1218:1278	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	1	15	theme	electrophoresis	191:205	arg1	method					212:217	A new capillary electrophoresis (CE) method	175:217	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector	175:281	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	4	16	theme	kV	1011:1012	arg1	voltage					997:1003	an applied voltage	986:1003	an applied voltage of 15 kV and containing 5% acetonitrile	986:1043	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	5	17	theme	linearity	1093:1101	arg1	terms					1080:1084	terms	1080:1084	terms of its linearity	1080:1101	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	4	18	theme	containing	1018:1027	arg1	acetonitrile					1032:1043	15 kV and containing 5% acetonitrile	1008:1043	acetonitrile	1032:1043	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	3	19	theme	intermediate	795:806	arg1	enantiomers					808:818	three intermediate enantiomers	789:818	its three intermediate enantiomers	785:818	Baseline and satisfactory separations were obtained for meptazinol and its three intermediate enantiomers.
23893795	4	20	theme	applied	989:995	arg1	voltage					997:1003	an applied voltage	986:1003	an applied voltage of 15 kV and containing 5% acetonitrile	986:1043	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	2	21	theme	organic	649:655	arg1	modifiers					657:665	the organic modifiers	645:665	the organic modifiers used	645:670	The primary factors affecting the separation efficiency, which include the chiral selector and its concentration, the buffer pH and composition, the organic modifiers used, and the applied voltage, were optimized.
23893795	0	22	theme	Enantiomeric	0:11	arg1	separation					13:22	Enantiomeric separation	0:22	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.	0:173	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.
23893795	0	23	theme	meptazinol	27:36	arg1	separation					13:22	Enantiomeric separation	0:22	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.	0:173	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.
23893795	4	24	theme	meptazinol	850:859	arg1	analysis					838:845	quantitative analysis	825:845	quantitative analysis of meptazinol	825:859	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	4	25	theme	%	1030:1030	arg1	acetonitrile					1032:1043	15 kV and containing 5% acetonitrile	1008:1043	acetonitrile	1032:1043	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	1	26	theme	enantiomeric	310:321	arg1	separation					323:332	the enantiomeric separation	306:332	the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV)	306:407	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	0	27	theme	meptazinol	132:141	arg1	analysis					120:127	quantitative analysis	107:127	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.	0:173	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.
23893795	1	28	from	meptazinol	477:486	arg1	tablets					491:497	tablets	491:497	tablets	491:497	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	5	29	theme	method	1070:1075	arg1	validation					1052:1061	validation	1052:1061	validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity	1052:1176	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	2	30	theme	primary	504:510	arg1	factors					512:518	The primary factors	500:518	The primary factors affecting the separation efficiency, which include the chiral selector and its concentration, the buffer pH and composition	500:642	The primary factors affecting the separation efficiency, which include the chiral selector and its concentration, the buffer pH and composition, the organic modifiers used, and the applied voltage, were optimized.
23893795	5	31	theme	quantitation	1128:1139	arg1	method					1070:1075	the method	1066:1075	the method in terms of its linearity	1066:1101	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	5	31	theme	quantitation	1128:1139	arg1	limits					1104:1109	limits	1104:1109	limits of detection and quantitation	1104:1139	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	0	32	theme	pharmaceutical	146:159	arg1	formulations					161:172	pharmaceutical formulations	146:172	pharmaceutical formulations	146:172	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.
23893795	4	33	theme	acetonitrile	1032:1043	arg1	voltage					997:1003	an applied voltage	986:1003	an applied voltage of 15 kV and containing 5% acetonitrile	986:1043	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	5	34	from	method	1070:1075	arg1	terms					1080:1084	terms	1080:1084	terms of its linearity	1080:1101	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	0	35	theme	intermediate	52:63	arg1	enantiomers					65:75	three intermediate enantiomers	46:75	its three intermediate enantiomers	42:75	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.
23893795	1	36	theme	meptazinol	337:346	arg1	separation					323:332	the enantiomeric separation	306:332	the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV)	306:407	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	2	37	theme	buffer	618:623	arg1	pH					625:626	the buffer pH	614:626	the buffer pH	614:626	The primary factors affecting the separation efficiency, which include the chiral selector and its concentration, the buffer pH and composition, the organic modifiers used, and the applied voltage, were optimized.
23893795	1	38	from	determination	460:472	arg1	tablets					491:497	tablets	491:497	tablets	491:497	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	5	39	theme	detection	1114:1122	arg1	method					1070:1075	the method	1066:1075	the method in terms of its linearity	1066:1101	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	5	39	theme	detection	1114:1122	arg1	limits					1104:1109	limits	1104:1109	limits of detection and quantitation	1104:1139	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	2	40	theme	chiral	575:580	arg1	selector					582:589	the chiral selector	571:589	the chiral selector	571:589	The primary factors affecting the separation efficiency, which include the chiral selector and its concentration, the buffer pH and composition, the organic modifiers used, and the applied voltage, were optimized.
23893795	1	41	dep	intermediates	388:400	arg1	II-IV					402:406	II-IV	402:406	intermediates II-IV	388:406	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	5	42	from	quantitation	1222:1233	arg1	formulations					1267:1278	pharmaceutical formulations	1252:1278	pharmaceutical formulations	1252:1278	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	4	43	theme	H3	942:943	arg1	buffer					949:954	20 mmol/L H3 PO4 buffer	932:954	20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile	932:1043	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	0	44	from	analysis	120:127	arg1	formulations					161:172	pharmaceutical formulations	146:172	pharmaceutical formulations	146:172	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.
23893795	4	45	theme	mmol/L	935:940	arg1	buffer					949:954	20 mmol/L H3 PO4 buffer	932:954	20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile	932:1043	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	1	46	theme	quantitative	447:458	arg1	determination					460:472	quantitative determination	447:472	quantitative determination of meptazinol in tablets	447:497	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	4	47	theme	quantitative	825:836	arg1	analysis					838:845	quantitative analysis	825:845	quantitative analysis of meptazinol	825:859	For quantitative analysis of meptazinol, the method was performed at the condition using 2.0 mmol/L CM-β-CD in 20 mmol/L H3 PO4 buffer adjusted to a pH of 6.00 with an applied voltage of 15 kV and containing 5% acetonitrile.
23893795	2	48	theme	separation	534:543	arg1	efficiency					545:554	the separation efficiency	530:554	the separation efficiency	530:554	The primary factors affecting the separation efficiency, which include the chiral selector and its concentration, the buffer pH and composition, the organic modifiers used, and the applied voltage, were optimized.
23893795	5	49	from	validation	1052:1061	arg1	terms					1080:1084	terms	1080:1084	terms of its linearity	1080:1101	After validation of the method in terms of its linearity, limits of detection and quantitation, accuracy, precision and selectivity, the method was successfully applied to the quantitation of meptazinol in pharmaceutical formulations.
23893795	1	50	theme	determination	460:472	arg1	application					432:442	the application	428:442	the application of quantitative determination of meptazinol in tablets	428:497	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	0	51	dep	separation	13:22	arg1	analysis					120:127	quantitative analysis	107:127	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.	0:173	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.
23893795	0	52	theme	enantiomers	65:75	arg1	separation					13:22	Enantiomeric separation	0:22	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.	0:173	Enantiomeric separation of meptazinol and its three intermediate enantiomers by capillary electrophoresis: quantitative analysis of meptazinol in pharmaceutical formulations.
23893795	3	53	theme	satisfactory	727:738	arg1	separations					740:750	satisfactory separations	727:750	satisfactory separations	727:750	Baseline and satisfactory separations were obtained for meptazinol and its three intermediate enantiomers.
23893795	1	54	from	tablets	491:497	arg1	determination					460:472	quantitative determination	447:472	quantitative determination of meptazinol in tablets	447:497	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
23893795	1	55	theme	chiral	267:272	arg1	selector					274:281	chiral selector	267:281	chiral selector	267:281	A new capillary electrophoresis (CE) method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has been developed for the enantiomeric separation of meptazinol and its three intermediate enantiomers (intermediates II-IV), and validated for the application of quantitative determination of meptazinol in tablets.
29080818	5	0	theme	injection	923:931	arg1	rate					933:936	injection rate	923:936	injection rate	923:936	In addition, the hydrodynamic diameter and loading capacity were significantly influenced by injection rate and pH of initial buffer solution.
29080818	8	1	theme	design	1325:1330	arg1	concept					1332:1338	design concept	1325:1338	design concept (QbD)	1325:1344	The results obviously showed that quality by design concept (QbD) could be effectively applied to optimize the developed ChS-nisin nanogel.
29080818	8	1	theme	design	1325:1330	arg1	QbD					1341:1343	QbD	1341:1343	QbD	1341:1343	The results obviously showed that quality by design concept (QbD) could be effectively applied to optimize the developed ChS-nisin nanogel.
29080818	7	2	theme	response	1250:1257	arg1	methodology					1267:1277	response surface methodology	1250:1277	response surface methodology	1250:1277	The observed values of dependent responses were close to predicted values confirmed by model from response surface methodology.
29080818	5	3	theme	buffer	956:961	arg1	solution					963:970	initial buffer solution	948:970	initial buffer solution	948:970	In addition, the hydrodynamic diameter and loading capacity were significantly influenced by injection rate and pH of initial buffer solution.
29080818	3	4	theme	nisin	532:536	arg1	ratio					523:527	concentration ratio	509:527	concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation	509:710	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	5	theme	injection	546:554	arg1	ChS					541:543	ChS	541:543	ChS	541:543	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	5	theme	injection	546:554	arg1	rate					556:559	injection rate	546:559	injection rate of nisin solution	546:577	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	6	theme	nisin	564:568	arg1	solution					570:577	nisin solution	564:577	nisin solution	564:577	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	0	7	theme	design	101:106	arg1	concept					108:114	design concept	101:114	design concept	101:114	Preparation and optimization of self-assembled chondroitin sulfate-nisin nanogel based on quality by design concept.
29080818	2	8	theme	critical	305:312	arg1	factors					314:320	The critical factors	301:320	The critical factors	301:320	The critical factors affected the physical properties of ChS-nisin nanogel was screened and optimized by Plackett-Burman design (PB) and central composite design (CCD).
29080818	6	9	theme	solution	1114:1121	arg1	rate					1133:1136	nisin solution injection rate	1108:1136	nisin solution injection rate of 0.2mL/min	1108:1149	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	7	10	theme	surface	1259:1265	arg1	methodology					1267:1277	response surface methodology	1250:1277	response surface methodology	1250:1277	The observed values of dependent responses were close to predicted values confirmed by model from response surface methodology.
29080818	5	11	theme	hydrodynamic	847:858	arg1	diameter					860:867	the hydrodynamic diameter	843:867	the hydrodynamic diameter	843:867	In addition, the hydrodynamic diameter and loading capacity were significantly influenced by injection rate and pH of initial buffer solution.
29080818	6	12	theme	buffer	1080:1085	arg1	solution					1087:1094	buffer solution	1080:1094	buffer solution at 4.6	1080:1101	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	5	13	theme	loading	873:879	arg1	capacity					881:888	loading capacity	873:888	loading capacity	873:888	In addition, the hydrodynamic diameter and loading capacity were significantly influenced by injection rate and pH of initial buffer solution.
29080818	6	14	from	ChS	1069:1071	arg1	4.6					1099:1101	4.6	1099:1101	4.6	1099:1101	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	4	15	theme	entrapment	772:781	arg1	efficiency					783:792	the entrapment efficiency	768:792	the entrapment efficiency	768:792	Among these factors, concentration ratio changed the entrapment efficiency and loading capacity significantly.
29080818	1	16	theme	charged	201:207	arg1	macromolecules					220:233	two oppositely charged biological macromolecules	186:233	two oppositely charged biological macromolecules	186:233	Self-assembled nanogel was prepared by electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS).
29080818	6	17	from	4.6	1099:1101	arg1	solution					1087:1094	buffer solution	1080:1094	buffer solution at 4.6	1080:1101	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	6	17	from	4.6	1099:1101	arg1	pH					1074:1075	pH	1074:1075	pH of buffer solution at 4.6	1074:1101	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	6	17	from	4.6	1099:1101	arg1	rate					1133:1136	nisin solution injection rate	1108:1136	nisin solution injection rate of 0.2mL/min	1108:1149	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	6	17	from	4.6	1099:1101	arg1	ChS					1069:1071	1mg/mL ChS	1062:1071	1mg/mL ChS	1062:1071	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	6	18	theme	optimized	977:985	arg1	structure					995:1003	The optimized nanogel structure	973:1003	The optimized nanogel structure	973:1003	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	8	19	theme	developed	1391:1399	arg1	nanogel					1411:1417	the developed ChS-nisin nanogel	1387:1417	the developed ChS-nisin nanogel	1387:1417	The results obviously showed that quality by design concept (QbD) could be effectively applied to optimize the developed ChS-nisin nanogel.
29080818	3	20	theme	solution	570:577	arg1	type					595:598	buffer solvent type	580:598	buffer solvent type	580:598	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	20	theme	solution	570:577	arg1	pH					625:626	pH	625:626	pH of initial buffer solution	625:653	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	20	theme	solution	570:577	arg1	ChS					541:543	ChS	541:543	ChS	541:543	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	20	theme	solution	570:577	arg1	rotation					703:710	centrifuge rotation	692:710	centrifuge rotation	692:710	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	20	theme	solution	570:577	arg1	rate					619:622	magnetic stirring rate	601:622	magnetic stirring rate	601:622	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	20	theme	solution	570:577	arg1	rate					556:559	injection rate	546:559	injection rate of nisin solution	546:577	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	20	theme	solution	570:577	arg1	temperature					675:685	centrifuge-cooling temperature	656:685	centrifuge-cooling temperature	656:685	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	1	21	theme	biological	209:218	arg1	macromolecules					220:233	two oppositely charged biological macromolecules	186:233	two oppositely charged biological macromolecules	186:233	Self-assembled nanogel was prepared by electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS).
29080818	7	22	theme	responses	1185:1193	arg1	values					1165:1170	The observed values	1152:1170	The observed values of dependent responses	1152:1193	The observed values of dependent responses were close to predicted values confirmed by model from response surface methodology.
29080818	7	23	theme	observed	1156:1163	arg1	values					1165:1170	The observed values	1152:1170	The observed values of dependent responses	1152:1193	The observed values of dependent responses were close to predicted values confirmed by model from response surface methodology.
29080818	6	24	from	pH	1074:1075	arg1	4.6					1099:1101	4.6	1099:1101	4.6	1099:1101	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	2	25	theme	physical	335:342	arg1	properties					344:353	the physical properties	331:353	the physical properties of ChS-nisin nanogel	331:374	The critical factors affected the physical properties of ChS-nisin nanogel was screened and optimized by Plackett-Burman design (PB) and central composite design (CCD).
29080818	2	26	theme	Plackett-Burman	406:420	arg1	PB					430:431	PB	430:431	PB	430:431	The critical factors affected the physical properties of ChS-nisin nanogel was screened and optimized by Plackett-Burman design (PB) and central composite design (CCD).
29080818	2	26	theme	Plackett-Burman	406:420	arg1	design					422:427	Plackett-Burman design	406:427	Plackett-Burman design (PB)	406:432	The critical factors affected the physical properties of ChS-nisin nanogel was screened and optimized by Plackett-Burman design (PB) and central composite design (CCD).
29080818	6	27	theme	0.2mL/min	1141:1149	arg1	pH					1074:1075	pH	1074:1075	pH of buffer solution at 4.6	1074:1101	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	6	27	theme	0.2mL/min	1141:1149	arg1	rate					1133:1136	nisin solution injection rate	1108:1136	nisin solution injection rate of 0.2mL/min	1108:1149	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	6	27	theme	0.2mL/min	1141:1149	arg1	ChS					1069:1071	1mg/mL ChS	1062:1071	1mg/mL ChS	1062:1071	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	8	28	theme	ChS-nisin	1401:1409	arg1	nanogel					1411:1417	the developed ChS-nisin nanogel	1387:1417	the developed ChS-nisin nanogel	1387:1417	The results obviously showed that quality by design concept (QbD) could be effectively applied to optimize the developed ChS-nisin nanogel.
29080818	2	29	theme	composite	446:454	arg1	CCD					464:466	CCD	464:466	CCD	464:466	The critical factors affected the physical properties of ChS-nisin nanogel was screened and optimized by Plackett-Burman design (PB) and central composite design (CCD).
29080818	2	29	theme	composite	446:454	arg1	design					456:461	central composite design	438:461	central composite design (CCD)	438:467	The critical factors affected the physical properties of ChS-nisin nanogel was screened and optimized by Plackett-Burman design (PB) and central composite design (CCD).
29080818	5	30	theme	solution	963:970	arg1	pH					942:943	pH	942:943	pH of initial buffer solution	942:970	In addition, the hydrodynamic diameter and loading capacity were significantly influenced by injection rate and pH of initial buffer solution.
29080818	5	30	theme	solution	963:970	arg1	rate					933:936	injection rate	923:936	injection rate	923:936	In addition, the hydrodynamic diameter and loading capacity were significantly influenced by injection rate and pH of initial buffer solution.
29080818	6	31	theme	nanogel	987:993	arg1	structure					995:1003	The optimized nanogel structure	973:1003	The optimized nanogel structure	973:1003	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	3	32	theme	initial	631:637	arg1	solution					646:653	initial buffer solution	631:653	initial buffer solution	631:653	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	1	33	theme	Self-assembled	117:130	arg1	nanogel					132:138	Self-assembled nanogel	117:138	Self-assembled nanogel	117:138	Self-assembled nanogel was prepared by electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS).
29080818	2	34	theme	central	438:444	arg1	CCD					464:466	CCD	464:466	CCD	464:466	The critical factors affected the physical properties of ChS-nisin nanogel was screened and optimized by Plackett-Burman design (PB) and central composite design (CCD).
29080818	2	34	theme	central	438:444	arg1	design					456:461	central composite design	438:461	central composite design (CCD)	438:467	The critical factors affected the physical properties of ChS-nisin nanogel was screened and optimized by Plackett-Burman design (PB) and central composite design (CCD).
29080818	0	35	theme	chondroitin	47:57	arg1	sulfate-nisin					59:71	self-assembled chondroitin sulfate-nisin	32:71	self-assembled chondroitin sulfate-nisin	32:71	Preparation and optimization of self-assembled chondroitin sulfate-nisin nanogel based on quality by design concept.
29080818	3	36	theme	concentration	509:521	arg1	ratio					523:527	concentration ratio	509:527	concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation	509:710	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	37	theme	buffer	639:644	arg1	solution					646:653	initial buffer solution	631:653	initial buffer solution	631:653	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	0	38	dep	nanogel	73:79	arg1	based					81:85	based	81:85	nanogel based on quality by design concept	73:114	Preparation and optimization of self-assembled chondroitin sulfate-nisin nanogel based on quality by design concept.
29080818	1	39	theme	macromolecules	220:233	arg1	complexation					170:181	electrostatic complexation	156:181	electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS)	156:298	Self-assembled nanogel was prepared by electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS).
29080818	0	40	theme	self-assembled	32:45	arg1	sulfate-nisin					59:71	self-assembled chondroitin sulfate-nisin	32:71	self-assembled chondroitin sulfate-nisin	32:71	Preparation and optimization of self-assembled chondroitin sulfate-nisin nanogel based on quality by design concept.
29080818	6	41	theme	concentration	1021:1033	arg1	ratio					1035:1039	concentration ratio	1021:1039	concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min	1021:1149	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	6	42	theme	6.4mg/mL	1044:1051	arg1	nisin					1053:1057	6.4mg/mL nisin	1044:1057	6.4mg/mL nisin	1044:1057	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	1	43	theme	cationic	247:254	arg1	nisin					256:260	cationic nisin	247:260	cationic nisin	247:260	Self-assembled nanogel was prepared by electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS).
29080818	3	44	theme	buffer	580:585	arg1	type					595:598	buffer solvent type	580:598	buffer solvent type	580:598	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	44	theme	buffer	580:585	arg1	ChS					541:543	ChS	541:543	ChS	541:543	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	6	45	theme	nisin	1108:1112	arg1	rate					1133:1136	nisin solution injection rate	1108:1136	nisin solution injection rate of 0.2mL/min	1108:1149	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	0	46	theme	sulfate-nisin	59:71	arg1	optimization					16:27	optimization	16:27	optimization	16:27	Preparation and optimization of self-assembled chondroitin sulfate-nisin nanogel based on quality by design concept.
29080818	0	46	theme	sulfate-nisin	59:71	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and optimization of self-assembled chondroitin sulfate-nisin nanogel based on quality by design concept.
29080818	3	47	theme	centrifuge-cooling	656:673	arg1	ChS					541:543	ChS	541:543	ChS	541:543	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	47	theme	centrifuge-cooling	656:673	arg1	temperature					675:685	centrifuge-cooling temperature	656:685	centrifuge-cooling temperature	656:685	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	48	theme	solvent	587:593	arg1	type					595:598	buffer solvent type	580:598	buffer solvent type	580:598	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	48	theme	solvent	587:593	arg1	ChS					541:543	ChS	541:543	ChS	541:543	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	7	49	theme	predicted	1209:1217	arg1	values					1219:1224	predicted values	1209:1224	predicted values	1209:1224	The observed values of dependent responses were close to predicted values confirmed by model from response surface methodology.
29080818	2	50	theme	nanogel	368:374	arg1	properties					344:353	the physical properties	331:353	the physical properties of ChS-nisin nanogel	331:374	The critical factors affected the physical properties of ChS-nisin nanogel was screened and optimized by Plackett-Burman design (PB) and central composite design (CCD).
29080818	4	51	theme	loading	798:804	arg1	capacity					806:813	loading capacity	798:813	loading capacity	798:813	Among these factors, concentration ratio changed the entrapment efficiency and loading capacity significantly.
29080818	6	52	theme	nisin	1053:1057	arg1	ratio					1035:1039	concentration ratio	1021:1039	concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min	1021:1149	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	2	53	theme	ChS-nisin	358:366	arg1	nanogel					368:374	ChS-nisin nanogel	358:374	ChS-nisin nanogel	358:374	The critical factors affected the physical properties of ChS-nisin nanogel was screened and optimized by Plackett-Burman design (PB) and central composite design (CCD).
29080818	6	54	from	rate	1133:1136	arg1	4.6					1099:1101	4.6	1099:1101	4.6	1099:1101	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	3	55	theme	solution	646:653	arg1	type					595:598	buffer solvent type	580:598	buffer solvent type	580:598	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	55	theme	solution	646:653	arg1	pH					625:626	pH	625:626	pH of initial buffer solution	625:653	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	55	theme	solution	646:653	arg1	ChS					541:543	ChS	541:543	ChS	541:543	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	55	theme	solution	646:653	arg1	rotation					703:710	centrifuge rotation	692:710	centrifuge rotation	692:710	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	55	theme	solution	646:653	arg1	rate					619:622	magnetic stirring rate	601:622	magnetic stirring rate	601:622	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	55	theme	solution	646:653	arg1	rate					556:559	injection rate	546:559	injection rate of nisin solution	546:577	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	55	theme	solution	646:653	arg1	temperature					675:685	centrifuge-cooling temperature	656:685	centrifuge-cooling temperature	656:685	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	4	56	theme	concentration	740:752	arg1	ratio					754:758	concentration ratio	740:758	concentration ratio	740:758	Among these factors, concentration ratio changed the entrapment efficiency and loading capacity significantly.
29080818	5	57	theme	initial	948:954	arg1	solution					963:970	initial buffer solution	948:970	initial buffer solution	948:970	In addition, the hydrodynamic diameter and loading capacity were significantly influenced by injection rate and pH of initial buffer solution.
29080818	6	58	theme	injection	1123:1131	arg1	rate					1133:1136	nisin solution injection rate	1108:1136	nisin solution injection rate of 0.2mL/min	1108:1149	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	6	59	theme	1mg/mL	1062:1067	arg1	ChS					1069:1071	1mg/mL ChS	1062:1071	1mg/mL ChS	1062:1071	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	3	60	theme	magnetic	601:608	arg1	ChS					541:543	ChS	541:543	ChS	541:543	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	60	theme	magnetic	601:608	arg1	rate					619:622	magnetic stirring rate	601:622	magnetic stirring rate	601:622	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	61	theme	independent	474:484	arg1	factors					486:492	The independent factors	470:492	The independent factors selected	470:501	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	1	62	theme	electrostatic	156:168	arg1	complexation					170:181	electrostatic complexation	156:181	electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS)	156:298	Self-assembled nanogel was prepared by electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS).
29080818	6	63	theme	solution	1087:1094	arg1	pH					1074:1075	pH	1074:1075	pH of buffer solution at 4.6	1074:1101	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	6	63	theme	solution	1087:1094	arg1	rate					1133:1136	nisin solution injection rate	1108:1136	nisin solution injection rate of 0.2mL/min	1108:1149	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	6	63	theme	solution	1087:1094	arg1	ChS					1069:1071	1mg/mL ChS	1062:1071	1mg/mL ChS	1062:1071	The optimized nanogel structure was obtained by concentration ratio of 6.4mg/mL nisin to 1mg/mL ChS, pH of buffer solution at 4.6, and nisin solution injection rate of 0.2mL/min.
29080818	1	64	theme	anionic	266:272	arg1	ChS					295:297	ChS	295:297	ChS	295:297	Self-assembled nanogel was prepared by electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS).
29080818	1	64	theme	anionic	266:272	arg1	sulfate					286:292	anionic chondroitin sulfate	266:292	anionic chondroitin sulfate (ChS)	266:298	Self-assembled nanogel was prepared by electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS).
29080818	3	65	theme	centrifuge	692:701	arg1	ChS					541:543	ChS	541:543	ChS	541:543	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	65	theme	centrifuge	692:701	arg1	rotation					703:710	centrifuge rotation	692:710	centrifuge rotation	692:710	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	66	theme	stirring	610:617	arg1	ChS					541:543	ChS	541:543	ChS	541:543	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	3	66	theme	stirring	610:617	arg1	rate					619:622	magnetic stirring rate	601:622	magnetic stirring rate	601:622	The independent factors selected were: concentration ratio of nisin to ChS, injection rate of nisin solution, buffer solvent type, magnetic stirring rate, pH of initial buffer solution, centrifuge-cooling temperature, and centrifuge rotation speed.
29080818	7	67	theme	dependent	1175:1183	arg1	responses					1185:1193	dependent responses	1175:1193	dependent responses	1175:1193	The observed values of dependent responses were close to predicted values confirmed by model from response surface methodology.
29080818	1	68	theme	chondroitin	274:284	arg1	ChS					295:297	ChS	295:297	ChS	295:297	Self-assembled nanogel was prepared by electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS).
29080818	1	68	theme	chondroitin	274:284	arg1	sulfate					286:292	anionic chondroitin sulfate	266:292	anionic chondroitin sulfate (ChS)	266:298	Self-assembled nanogel was prepared by electrostatic complexation of two oppositely charged biological macromolecules, which were cationic nisin and anionic chondroitin sulfate (ChS).
26656812	2	0	theme	quasi-reversible	421:436	arg1	CV					459:460	CV	459:460	CV	459:460	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	0	theme	quasi-reversible	421:436	arg1	voltammetric					445:456	quasi-reversible cyclic voltammetric	421:456	quasi-reversible cyclic voltammetric (CV)	421:461	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	1	theme	characteristic	513:526	arg1	redox					558:562	characteristic of Mb heme Fe((III))/Fe((II)) redox	513:562	characteristic of Mb heme Fe((III))/Fe((II)) redox couples	513:570	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	1	theme	characteristic	513:526	arg1	buffers					504:510	pH 7.0 buffers	497:510	pH 7.0 buffers	497:510	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	3	2	theme	heterogeneous	652:664	arg1	ks					700:701	ks	700:701	ks	700:701	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	3	2	theme	heterogeneous	652:664	arg1	constants					689:697	apparent heterogeneous electron-transfer rate constants	643:697	apparent heterogeneous electron-transfer rate constants (ks)	643:702	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	5	3	theme	absorbance	882:891	arg1	band					893:896	the Soret absorbance band	872:896	the Soret absorbance band	872:896	The positions of the Soret absorbance band suggest that Mb in the CA's composite film kept its native states in the medium pH range.
26656812	3	4	theme	electron-transfer	666:682	arg1	ks					700:701	ks	700:701	ks	700:701	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	3	4	theme	electron-transfer	666:682	arg1	constants					689:697	apparent heterogeneous electron-transfer rate constants	643:697	apparent heterogeneous electron-transfer rate constants (ks)	643:702	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	2	5	theme	peaks	463:467	arg1	pair					399:402	a pair	397:402	a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks	397:467	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	5	6	theme	native	950:955	arg1	states					957:962	its native states	946:962	its native states	946:962	The positions of the Soret absorbance band suggest that Mb in the CA's composite film kept its native states in the medium pH range.
26656812	6	7	theme	composite	1075:1083	arg1	film					1085:1088	the Mb-CA's composite film	1063:1088	the Mb-CA's composite film with significant lowering of the reduction overpotential	1063:1145	Hydrogen peroxide, oxygen, and nitrite were electrochemically catalyzed by the Mb-CA's composite film with significant lowering of the reduction overpotential.
26656812	0	8	with	Film	77:80	arg1	Alcohol					97:103	Polyvinyl Alcohol	87:103	Polyvinyl Alcohol	87:103	Electroactive Film of Myoglobin Incorporated in a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin.
26656812	0	8	with	Film	77:80	arg1	Gelatin					119:125	Gelatin	119:125	Gelatin	119:125	Electroactive Film of Myoglobin Incorporated in a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin.
26656812	0	8	with	Film	77:80	arg1	Glycerin					106:113	Glycerin	106:113	Glycerin	106:113	Electroactive Film of Myoglobin Incorporated in a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin.
26656812	6	9	theme	Hydrogen	988:995	arg1	peroxide					997:1004	Hydrogen peroxide	988:1004	Hydrogen peroxide	988:1004	Hydrogen peroxide, oxygen, and nitrite were electrochemically catalyzed by the Mb-CA's composite film with significant lowering of the reduction overpotential.
26656812	0	10	theme	Polyvinyl	87:95	arg1	Alcohol					97:103	Polyvinyl Alcohol	87:103	Polyvinyl Alcohol	87:103	Electroactive Film of Myoglobin Incorporated in a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin.
26656812	1	11	theme	polyvinyl	301:309	arg1	alcohol					311:317	polyvinyl alcohol	301:317	polyvinyl alcohol	301:317	In this work, an electroactive porous Mb-CA's composite film was fabricated by incorporating myoglobin (Mb) in a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin.
26656812	3	12	theme	nonlinear	752:760	arg1	analysis					773:780	nonlinear regression analysis	752:780	nonlinear regression analysis	752:780	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	6	13	theme	overpotential	1133:1145	arg1	lowering					1107:1114	significant lowering	1095:1114	significant lowering of the reduction overpotential	1095:1145	Hydrogen peroxide, oxygen, and nitrite were electrochemically catalyzed by the Mb-CA's composite film with significant lowering of the reduction overpotential.
26656812	3	14	theme	apparent	643:650	arg1	ks					700:701	ks	700:701	ks	700:701	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	3	14	theme	apparent	643:650	arg1	constants					689:697	apparent heterogeneous electron-transfer rate constants	643:697	apparent heterogeneous electron-transfer rate constants (ks)	643:702	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	5	15	from	Mb	911:912	arg1	film					936:939	the CA's composite film	917:939	the CA's composite film	917:939	The positions of the Soret absorbance band suggest that Mb in the CA's composite film kept its native states in the medium pH range.
26656812	3	16	theme	electrochemical	577:591	arg1	potentials					620:629	formal potentials	613:629	formal potentials (E(o'))	613:637	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	3	16	theme	electrochemical	577:591	arg1	parameters					593:602	The electrochemical parameters	573:602	The electrochemical parameters	573:602	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	3	16	theme	electrochemical	577:591	arg1	constants					689:697	apparent heterogeneous electron-transfer rate constants	643:697	apparent heterogeneous electron-transfer rate constants (ks)	643:702	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	2	17	theme	Fe	539:540	arg1	characteristic					513:526	characteristic	513:526	characteristic	513:526	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	1	18	theme	electroactive	145:157	arg1	film					184:187	an electroactive porous Mb-CA's composite film	142:187	an electroactive porous Mb-CA's composite film	142:187	In this work, an electroactive porous Mb-CA's composite film was fabricated by incorporating myoglobin (Mb) in a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin.
26656812	2	19	theme	heme	534:537	arg1	Fe					539:540	Mb heme Fe	531:540	Mb heme Fe((III))	531:547	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	3	20	dep	potentials	620:629	arg1	o'					634:635	o'	634:635	o'	634:635	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	3	20	dep	potentials	620:629	arg1	E					632:632	E	632:632	E(o')	632:636	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	1	21	theme	porous	159:164	arg1	film					184:187	an electroactive porous Mb-CA's composite film	142:187	an electroactive porous Mb-CA's composite film	142:187	In this work, an electroactive porous Mb-CA's composite film was fabricated by incorporating myoglobin (Mb) in a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin.
26656812	5	22	theme	composite	926:934	arg1	film					936:939	the CA's composite film	917:939	the CA's composite film	917:939	The positions of the Soret absorbance band suggest that Mb in the CA's composite film kept its native states in the medium pH range.
26656812	2	23	theme	Mb	531:532	arg1	Fe					539:540	Mb heme Fe	531:540	Mb heme Fe((III))	531:547	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	4	24	theme	porous	787:792	arg1	film					809:812	The porous CA's composite film	783:812	The porous CA's composite film	783:812	The porous CA's composite film could form hydrogel in aqueous solution.
26656812	3	25	theme	square-wave	723:733	arg1	voltammetry					735:745	square-wave voltammetry	723:745	square-wave voltammetry with nonlinear regression analysis	723:780	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	0	26	theme	Electroactive	0:12	arg1	Film					14:17	Electroactive Film	0:17	Electroactive Film of Myoglobin	0:30	Electroactive Film of Myoglobin Incorporated in a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin.
26656812	5	27	theme	pH	978:979	arg1	range					981:985	the medium pH range	967:985	the medium pH range	967:985	The positions of the Soret absorbance band suggest that Mb in the CA's composite film kept its native states in the medium pH range.
26656812	2	28	theme	/Fe	548:550	arg1	redox					558:562	characteristic of Mb heme Fe((III))/Fe((II)) redox	513:562	characteristic of Mb heme Fe((III))/Fe((II)) redox couples	513:570	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	28	theme	/Fe	548:550	arg1	buffers					504:510	pH 7.0 buffers	497:510	pH 7.0 buffers	497:510	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	0	29	theme	Myoglobin	22:30	arg1	Film					14:17	Electroactive Film	0:17	Electroactive Film of Myoglobin	0:30	Electroactive Film of Myoglobin Incorporated in a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin.
26656812	1	30	theme	composite	174:182	arg1	film					184:187	an electroactive porous Mb-CA's composite film	142:187	an electroactive porous Mb-CA's composite film	142:187	In this work, an electroactive porous Mb-CA's composite film was fabricated by incorporating myoglobin (Mb) in a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin.
26656812	2	31	theme	film	362:365	arg1	electrodes					376:385	The porous Mb-CA's film modified electrodes	343:385	The porous Mb-CA's film modified electrodes	343:385	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	32	dep	redox	558:562	arg1	II					553:554	II	553:554	II	553:554	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	32	dep	redox	558:562	arg1	couples					564:570	couples	564:570	couples	564:570	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	33	theme	well-defined	407:418	arg1	peaks					463:467	well-defined, quasi-reversible cyclic voltammetric (CV) peaks	407:467	peaks	463:467	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	34	dep	peaks	463:467	arg1	CV					459:460	CV	459:460	CV	459:460	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	34	dep	peaks	463:467	arg1	voltammetric					445:456	quasi-reversible cyclic voltammetric	421:456	quasi-reversible cyclic voltammetric (CV)	421:461	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	3	35	theme	regression	762:771	arg1	analysis					773:780	nonlinear regression analysis	752:780	nonlinear regression analysis	752:780	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	2	36	theme	porous	347:352	arg1	electrodes					376:385	The porous Mb-CA's film modified electrodes	343:385	The porous Mb-CA's film modified electrodes	343:385	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	1	37	theme	porous	262:267	arg1	film					291:294	a three-dimension (3D) porous calcium alginate (CA) film	239:294	a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin	239:340	In this work, an electroactive porous Mb-CA's composite film was fabricated by incorporating myoglobin (Mb) in a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin.
26656812	6	38	theme	reduction	1123:1131	arg1	overpotential					1133:1145	the reduction overpotential	1119:1145	the reduction overpotential	1119:1145	Hydrogen peroxide, oxygen, and nitrite were electrochemically catalyzed by the Mb-CA's composite film with significant lowering of the reduction overpotential.
26656812	3	39	theme	formal	613:618	arg1	potentials					620:629	formal potentials	613:629	formal potentials (E(o'))	613:637	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	2	40	theme	modified	367:374	arg1	electrodes					376:385	The porous Mb-CA's film modified electrodes	343:385	The porous Mb-CA's film modified electrodes	343:385	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	0	41	theme	3D-porous	50:58	arg1	Film					77:80	a 3D-porous Calcium Alginate Film	48:80	a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin	48:125	Electroactive Film of Myoglobin Incorporated in a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin.
26656812	1	42	theme	calcium	269:275	arg1	film					291:294	a three-dimension (3D) porous calcium alginate (CA) film	239:294	a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin	239:340	In this work, an electroactive porous Mb-CA's composite film was fabricated by incorporating myoglobin (Mb) in a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin.
26656812	2	43	theme	pH	497:498	arg1	redox					558:562	characteristic of Mb heme Fe((III))/Fe((II)) redox	513:562	characteristic of Mb heme Fe((III))/Fe((II)) redox couples	513:570	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	43	theme	pH	497:498	arg1	buffers					504:510	pH 7.0 buffers	497:510	pH 7.0 buffers	497:510	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	44	theme	cyclic	438:443	arg1	CV					459:460	CV	459:460	CV	459:460	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	44	theme	cyclic	438:443	arg1	voltammetric					445:456	quasi-reversible cyclic voltammetric	421:456	quasi-reversible cyclic voltammetric (CV)	421:461	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	4	45	theme	aqueous	837:843	arg1	solution					845:852	aqueous solution	837:852	aqueous solution	837:852	The porous CA's composite film could form hydrogel in aqueous solution.
26656812	1	46	theme	alginate	277:284	arg1	film					291:294	a three-dimension (3D) porous calcium alginate (CA) film	239:294	a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin	239:340	In this work, an electroactive porous Mb-CA's composite film was fabricated by incorporating myoglobin (Mb) in a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin.
26656812	4	47	theme	composite	799:807	arg1	film					809:812	The porous CA's composite film	783:812	The porous CA's composite film	783:812	The porous CA's composite film could form hydrogel in aqueous solution.
26656812	5	48	theme	medium	971:976	arg1	range					981:985	the medium pH range	967:985	the medium pH range	967:985	The positions of the Soret absorbance band suggest that Mb in the CA's composite film kept its native states in the medium pH range.
26656812	5	49	theme	Soret	876:880	arg1	band					893:896	the Soret absorbance band	872:896	the Soret absorbance band	872:896	The positions of the Soret absorbance band suggest that Mb in the CA's composite film kept its native states in the medium pH range.
26656812	6	50	with	film	1085:1088	arg1	lowering					1107:1114	significant lowering	1095:1114	significant lowering of the reduction overpotential	1095:1145	Hydrogen peroxide, oxygen, and nitrite were electrochemically catalyzed by the Mb-CA's composite film with significant lowering of the reduction overpotential.
26656812	6	51	theme	significant	1095:1105	arg1	lowering					1107:1114	significant lowering	1095:1114	significant lowering of the reduction overpotential	1095:1145	Hydrogen peroxide, oxygen, and nitrite were electrochemically catalyzed by the Mb-CA's composite film with significant lowering of the reduction overpotential.
26656812	3	52	with	voltammetry	735:745	arg1	analysis					773:780	nonlinear regression analysis	752:780	nonlinear regression analysis	752:780	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	3	53	theme	rate	684:687	arg1	ks					700:701	ks	700:701	ks	700:701	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	3	53	theme	rate	684:687	arg1	constants					689:697	apparent heterogeneous electron-transfer rate constants	643:697	apparent heterogeneous electron-transfer rate constants (ks)	643:702	The electrochemical parameters, such as formal potentials (E(o')) and apparent heterogeneous electron-transfer rate constants (ks), were estimated by square-wave voltammetry with nonlinear regression analysis.
26656812	0	54	theme	Alginate	68:75	arg1	Film					77:80	a 3D-porous Calcium Alginate Film	48:80	a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin	48:125	Electroactive Film of Myoglobin Incorporated in a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin.
26656812	5	55	theme	band	893:896	arg1	positions					859:867	The positions	855:867	The positions of the Soret absorbance band	855:896	The positions of the Soret absorbance band suggest that Mb in the CA's composite film kept its native states in the medium pH range.
26656812	2	56	dep	Fe	539:540	arg1	III					543:545	III	543:545	III	543:545	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	57	from	V	484:484	arg1	redox					558:562	characteristic of Mb heme Fe((III))/Fe((II)) redox	513:562	characteristic of Mb heme Fe((III))/Fe((II)) redox couples	513:570	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	2	57	from	V	484:484	arg1	buffers					504:510	pH 7.0 buffers	497:510	pH 7.0 buffers	497:510	The porous Mb-CA's film modified electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.37 V vs. SCE in pH 7.0 buffers, characteristic of Mb heme Fe((III))/Fe((II)) redox couples.
26656812	0	58	theme	Calcium	60:66	arg1	Film					77:80	a 3D-porous Calcium Alginate Film	48:80	a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin	48:125	Electroactive Film of Myoglobin Incorporated in a 3D-porous Calcium Alginate Film with Polyvinyl Alcohol, Glycerin and Gelatin.
26656812	1	59	theme	CA	287:288	arg1	film					291:294	a three-dimension (3D) porous calcium alginate (CA) film	239:294	a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin	239:340	In this work, an electroactive porous Mb-CA's composite film was fabricated by incorporating myoglobin (Mb) in a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin.
26656812	1	60	with	film	291:294	arg1	gelatin					334:340	gelatin	334:340	gelatin	334:340	In this work, an electroactive porous Mb-CA's composite film was fabricated by incorporating myoglobin (Mb) in a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin.
26656812	1	60	with	film	291:294	arg1	alcohol					311:317	polyvinyl alcohol	301:317	polyvinyl alcohol	301:317	In this work, an electroactive porous Mb-CA's composite film was fabricated by incorporating myoglobin (Mb) in a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin.
26656812	1	60	with	film	291:294	arg1	glycerol					320:327	glycerol	320:327	glycerol	320:327	In this work, an electroactive porous Mb-CA's composite film was fabricated by incorporating myoglobin (Mb) in a three-dimension (3D) porous calcium alginate (CA) film with polyvinyl alcohol, glycerol, and gelatin.
29131597	0	0	theme	Scavenging	105:114	arg1	Property					116:123	Its Radical Scavenging Property	93:123	Its Radical Scavenging Property in Vitro	93:132	Facile Low-Temperature Synthesis of Cellulose Nanocrystals Carrying Buckminsterfullerene and Its Radical Scavenging Property in Vitro.
29131597	2	1	theme	drug	362:365	arg1	carriers					376:383	drug delivery carriers	362:383	drug delivery carriers based on nanoparticle technologies	362:418	Its clinical use would be promoted by novel functionalization of C60 with the aid of drug delivery carriers based on nanoparticle technologies.
29131597	0	2	theme	Radical	97:103	arg1	Property					116:123	Its Radical Scavenging Property	93:123	Its Radical Scavenging Property in Vitro	93:132	Facile Low-Temperature Synthesis of Cellulose Nanocrystals Carrying Buckminsterfullerene and Its Radical Scavenging Property in Vitro.
29131597	6	3	theme	equivalent	1105:1114	arg1	content					1070:1076	a C60 content	1064:1076	a C60 content	1064:1076	Given atomic compositions of samples by elemental analysis, we figured out a C60 content of 0.17 mmol/g of CNC-C60, equivalent to 34 C60 molecules/1000 anhydroglucose units (AGU).
29131597	7	4	theme	antiradical	1210:1220	arg1	effects					1222:1228	its antiradical effects	1206:1228	its antiradical effects on scavenging hydroxyl radicals	1206:1260	Afterward, CNC-C60 was evaluated for its antiradical effects on scavenging hydroxyl radicals in vitro.
29131597	8	5	dep	CNC-C60	1343:1349	arg1	agent					1381:1385	a promising novel antioxidant agent	1351:1385	CNC-C60 a promising novel antioxidant agent for biomedical use	1343:1404	The ease of synthesis and significant radical scavenging activity make CNC-C60 a promising novel antioxidant agent for biomedical use.
29131597	4	6	theme	grafting	809:816	arg1	functionalization					768:784	amine functionalization	762:784	amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs	762:865	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	4	7	theme	CNCs	789:792	arg1	functionalization					768:784	amine functionalization	762:784	amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs	762:865	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	6	8	theme	elemental	1029:1037	arg1	analysis					1039:1046	elemental analysis	1029:1046	elemental analysis	1029:1046	Given atomic compositions of samples by elemental analysis, we figured out a C60 content of 0.17 mmol/g of CNC-C60, equivalent to 34 C60 molecules/1000 anhydroglucose units (AGU).
29131597	8	9	theme	novel	1363:1367	arg1	agent					1381:1385	a promising novel antioxidant agent	1351:1385	CNC-C60 a promising novel antioxidant agent for biomedical use	1343:1404	The ease of synthesis and significant radical scavenging activity make CNC-C60 a promising novel antioxidant agent for biomedical use.
29131597	4	10	theme	subsequent	798:807	arg1	grafting					809:816	subsequent grafting	798:816	subsequent grafting of C60	798:823	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	2	11	with	functionalization	321:337	arg1	aid					355:357	aid	355:357	aid	355:357	Its clinical use would be promoted by novel functionalization of C60 with the aid of drug delivery carriers based on nanoparticle technologies.
29131597	8	12	theme	significant	1298:1308	arg1	activity					1329:1336	significant radical scavenging activity	1298:1336	significant radical scavenging activity	1298:1336	The ease of synthesis and significant radical scavenging activity make CNC-C60 a promising novel antioxidant agent for biomedical use.
29131597	4	13	theme	C60	821:823	arg1	CNCs					789:792	CNCs	789:792	CNCs	789:792	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	4	13	theme	C60	821:823	arg1	grafting					809:816	subsequent grafting	798:816	subsequent grafting of C60	798:823	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	1	14	theme	strong	177:182	arg1	properties					203:212	its strong radical scavenging properties	173:212	its strong radical scavenging properties	173:212	Buckminsterfullerene (C60), known for its strong radical scavenging properties, has been studied extensively for its biomedical applications.
29131597	6	15	theme	anhydroglucose	1141:1154	arg1	AGU					1163:1165	AGU	1163:1165	AGU	1163:1165	Given atomic compositions of samples by elemental analysis, we figured out a C60 content of 0.17 mmol/g of CNC-C60, equivalent to 34 C60 molecules/1000 anhydroglucose units (AGU).
29131597	6	15	theme	anhydroglucose	1141:1154	arg1	units					1156:1160	34 C60 molecules/1000 anhydroglucose units	1119:1160	34 C60 molecules/1000 anhydroglucose units (AGU)	1119:1166	Given atomic compositions of samples by elemental analysis, we figured out a C60 content of 0.17 mmol/g of CNC-C60, equivalent to 34 C60 molecules/1000 anhydroglucose units (AGU).
29131597	3	16	theme	intriguing	542:551	arg1	attributes					553:562	their intriguing attributes	536:562	their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility	536:662	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	3	16	theme	intriguing	542:551	arg1	capacity					633:640	broad chemical-modifying capacity	608:640	broad chemical-modifying capacity	608:640	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	3	16	theme	intriguing	542:551	arg1	biocompatibility					647:662	biocompatibility	647:662	biocompatibility	647:662	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	3	16	theme	intriguing	542:551	arg1	dimensions					582:591	nanoscale dimensions	572:591	nanoscale dimensions	572:591	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	3	16	theme	intriguing	542:551	arg1	toxicity					598:605	low toxicity	594:605	low toxicity	594:605	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	1	17	theme	radical	184:190	arg1	properties					203:212	its strong radical scavenging properties	173:212	its strong radical scavenging properties	173:212	Buckminsterfullerene (C60), known for its strong radical scavenging properties, has been studied extensively for its biomedical applications.
29131597	0	18	theme	Low-Temperature	7:21	arg1	Synthesis					23:31	Facile Low-Temperature Synthesis	0:31	Facile Low-Temperature Synthesis of Cellulose Nanocrystals Carrying Buckminsterfullerene and Its Radical Scavenging Property in Vitro.	0:133	Facile Low-Temperature Synthesis of Cellulose Nanocrystals Carrying Buckminsterfullerene and Its Radical Scavenging Property in Vitro.
29131597	7	19	theme	scavenging	1233:1242	arg1	radicals					1253:1260	scavenging hydroxyl radicals	1233:1260	scavenging hydroxyl radicals	1233:1260	Afterward, CNC-C60 was evaluated for its antiradical effects on scavenging hydroxyl radicals in vitro.
29131597	4	20	theme	cellulose	673:681	arg1	nanocrystals					683:694	cellulose nanocrystals	673:694	cellulose nanocrystals carrying buckminsterfullerene (CNC-C60)	673:734	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	5	21	theme	TGA	975:977	arg1	analysis					979:986	TGA analysis	975:986	TGA analysis	975:986	FTIR and XPS measurements confirmed the success of the synthesis, which was further evidently supported by TGA analysis.
29131597	1	22	theme	scavenging	192:201	arg1	properties					203:212	its strong radical scavenging properties	173:212	its strong radical scavenging properties	173:212	Buckminsterfullerene (C60), known for its strong radical scavenging properties, has been studied extensively for its biomedical applications.
29131597	0	23	theme	Facile	0:5	arg1	Synthesis					23:31	Facile Low-Temperature Synthesis	0:31	Facile Low-Temperature Synthesis of Cellulose Nanocrystals Carrying Buckminsterfullerene and Its Radical Scavenging Property in Vitro.	0:133	Facile Low-Temperature Synthesis of Cellulose Nanocrystals Carrying Buckminsterfullerene and Its Radical Scavenging Property in Vitro.
29131597	6	24	theme	samples	1018:1024	arg1	compositions					1002:1013	atomic compositions	995:1013	atomic compositions of samples by elemental analysis	995:1046	Given atomic compositions of samples by elemental analysis, we figured out a C60 content of 0.17 mmol/g of CNC-C60, equivalent to 34 C60 molecules/1000 anhydroglucose units (AGU).
29131597	8	25	theme	biomedical	1391:1400	arg1	use					1402:1404	biomedical use	1391:1404	biomedical use	1391:1404	The ease of synthesis and significant radical scavenging activity make CNC-C60 a promising novel antioxidant agent for biomedical use.
29131597	6	26	theme	mmol/g	1086:1091	arg1	content					1070:1076	a C60 content	1064:1076	a C60 content	1064:1076	Given atomic compositions of samples by elemental analysis, we figured out a C60 content of 0.17 mmol/g of CNC-C60, equivalent to 34 C60 molecules/1000 anhydroglucose units (AGU).
29131597	6	27	theme	CNC-C60	1096:1102	arg1	mmol/g					1086:1091	0.17 mmol/g	1081:1091	0.17 mmol/g of CNC-C60	1081:1102	Given atomic compositions of samples by elemental analysis, we figured out a C60 content of 0.17 mmol/g of CNC-C60, equivalent to 34 C60 molecules/1000 anhydroglucose units (AGU).
29131597	2	28	theme	novel	315:319	arg1	functionalization					321:337	novel functionalization	315:337	novel functionalization of C60 with the aid of drug delivery carriers based on nanoparticle technologies	315:418	Its clinical use would be promoted by novel functionalization of C60 with the aid of drug delivery carriers based on nanoparticle technologies.
29131597	5	29	theme	FTIR	868:871	arg1	measurements					881:892	FTIR and XPS measurements	868:892	FTIR and XPS measurements	868:892	FTIR and XPS measurements confirmed the success of the synthesis, which was further evidently supported by TGA analysis.
29131597	3	30	theme	nanoscale	572:580	arg1	dimensions					582:591	nanoscale dimensions	572:591	nanoscale dimensions	572:591	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	5	31	theme	XPS	877:879	arg1	measurements					881:892	FTIR and XPS measurements	868:892	FTIR and XPS measurements	868:892	FTIR and XPS measurements confirmed the success of the synthesis, which was further evidently supported by TGA analysis.
29131597	0	32	theme	Nanocrystals	46:57	arg1	Synthesis					23:31	Facile Low-Temperature Synthesis	0:31	Facile Low-Temperature Synthesis of Cellulose Nanocrystals Carrying Buckminsterfullerene and Its Radical Scavenging Property in Vitro.	0:133	Facile Low-Temperature Synthesis of Cellulose Nanocrystals Carrying Buckminsterfullerene and Its Radical Scavenging Property in Vitro.
29131597	6	33	theme	atomic	995:1000	arg1	compositions					1002:1013	atomic compositions	995:1013	atomic compositions of samples by elemental analysis	995:1046	Given atomic compositions of samples by elemental analysis, we figured out a C60 content of 0.17 mmol/g of CNC-C60, equivalent to 34 C60 molecules/1000 anhydroglucose units (AGU).
29131597	4	34	theme	CNCs	862:865	arg1	surface					834:840	the surface	830:840	the surface of amine-terminated CNCs	830:865	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	8	35	theme	activity	1329:1336	arg1	ease					1276:1279	The ease	1272:1279	The ease of synthesis and significant radical scavenging activity	1272:1336	The ease of synthesis and significant radical scavenging activity make CNC-C60 a promising novel antioxidant agent for biomedical use.
29131597	0	36	theme	Cellulose	36:44	arg1	Nanocrystals					46:57	Cellulose Nanocrystals	36:57	Cellulose Nanocrystals Carrying Buckminsterfullerene and Its Radical Scavenging Property in Vitro	36:132	Facile Low-Temperature Synthesis of Cellulose Nanocrystals Carrying Buckminsterfullerene and Its Radical Scavenging Property in Vitro.
29131597	3	37	theme	broad	608:612	arg1	capacity					633:640	broad chemical-modifying capacity	608:640	broad chemical-modifying capacity	608:640	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	8	38	theme	scavenging	1318:1327	arg1	activity					1329:1336	significant radical scavenging activity	1298:1336	significant radical scavenging activity	1298:1336	The ease of synthesis and significant radical scavenging activity make CNC-C60 a promising novel antioxidant agent for biomedical use.
29131597	7	39	from	effects	1222:1228	arg1	radicals					1253:1260	scavenging hydroxyl radicals	1233:1260	scavenging hydroxyl radicals	1233:1260	Afterward, CNC-C60 was evaluated for its antiradical effects on scavenging hydroxyl radicals in vitro.
29131597	3	40	theme	low	594:596	arg1	toxicity					598:605	low toxicity	594:605	low toxicity	594:605	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	8	41	theme	radical	1310:1316	arg1	activity					1329:1336	significant radical scavenging activity	1298:1336	significant radical scavenging activity	1298:1336	The ease of synthesis and significant radical scavenging activity make CNC-C60 a promising novel antioxidant agent for biomedical use.
29131597	3	42	theme	Cellulose	421:429	arg1	CNCs					445:448	CNCs	445:448	CNCs	445:448	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	3	42	theme	Cellulose	421:429	arg1	nanocrystals					431:442	Cellulose nanocrystals	421:442	Cellulose nanocrystals (CNCs)	421:449	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	3	42	theme	Cellulose	421:429	arg1	nanoplatform					495:506	a promising nanoplatform	483:506	a promising nanoplatform for drug delivery	483:524	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	4	43	contain	carrying	696:703	arg2	buckminsterfullerene					705:724	buckminsterfullerene	705:724	buckminsterfullerene (CNC-C60)	705:734	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	4	43	contain	carrying	696:703	arg1	nanocrystals					683:694	cellulose nanocrystals	673:694	cellulose nanocrystals carrying buckminsterfullerene (CNC-C60)	673:734	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	4	43	contain	carrying	696:703	arg2	CNC-C60					727:733	CNC-C60	727:733	CNC-C60	727:733	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	3	44	theme	drug	512:515	arg1	delivery					517:524	drug delivery	512:524	drug delivery	512:524	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	2	45	theme	clinical	281:288	arg1	use					290:292	Its clinical use	277:292	Its clinical use	277:292	Its clinical use would be promoted by novel functionalization of C60 with the aid of drug delivery carriers based on nanoparticle technologies.
29131597	4	46	theme	amine-terminated	845:860	arg1	CNCs					862:865	amine-terminated CNCs	845:865	amine-terminated CNCs	845:865	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	3	47	theme	promising	485:493	arg1	nanocrystals					431:442	Cellulose nanocrystals	421:442	Cellulose nanocrystals (CNCs)	421:449	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	3	47	theme	promising	485:493	arg1	nanoplatform					495:506	a promising nanoplatform	483:506	a promising nanoplatform for drug delivery	483:524	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	8	48	theme	antioxidant	1369:1379	arg1	agent					1381:1385	a promising novel antioxidant agent	1351:1385	CNC-C60 a promising novel antioxidant agent for biomedical use	1343:1404	The ease of synthesis and significant radical scavenging activity make CNC-C60 a promising novel antioxidant agent for biomedical use.
29131597	2	49	theme	C60	342:344	arg1	functionalization					321:337	novel functionalization	315:337	novel functionalization of C60 with the aid of drug delivery carriers based on nanoparticle technologies	315:418	Its clinical use would be promoted by novel functionalization of C60 with the aid of drug delivery carriers based on nanoparticle technologies.
29131597	8	50	theme	promising	1353:1361	arg1	agent					1381:1385	a promising novel antioxidant agent	1351:1385	CNC-C60 a promising novel antioxidant agent for biomedical use	1343:1404	The ease of synthesis and significant radical scavenging activity make CNC-C60 a promising novel antioxidant agent for biomedical use.
29131597	4	51	theme	amine	762:766	arg1	functionalization					768:784	amine functionalization	762:784	amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs	762:865	Herein, cellulose nanocrystals carrying buckminsterfullerene (CNC-C60) have been synthesized via amine functionalization of CNCs and subsequent grafting of C60 onto the surface of amine-terminated CNCs.
29131597	7	52	theme	hydroxyl	1244:1251	arg1	radicals					1253:1260	scavenging hydroxyl radicals	1233:1260	scavenging hydroxyl radicals	1233:1260	Afterward, CNC-C60 was evaluated for its antiradical effects on scavenging hydroxyl radicals in vitro.
29131597	8	53	theme	synthesis	1284:1292	arg1	ease					1276:1279	The ease	1272:1279	The ease of synthesis and significant radical scavenging activity	1272:1336	The ease of synthesis and significant radical scavenging activity make CNC-C60 a promising novel antioxidant agent for biomedical use.
29131597	3	54	theme	chemical-modifying	614:631	arg1	capacity					633:640	broad chemical-modifying capacity	608:640	broad chemical-modifying capacity	608:640	Cellulose nanocrystals (CNCs) have recently been exploited as a promising nanoplatform for drug delivery, owing to their intriguing attributes such as nanoscale dimensions, low toxicity, broad chemical-modifying capacity, and biocompatibility.
29131597	5	55	theme	synthesis	923:931	arg1	success					908:914	the success	904:914	the success of the synthesis, which was further evidently supported by TGA analysis	904:986	FTIR and XPS measurements confirmed the success of the synthesis, which was further evidently supported by TGA analysis.
29131597	2	56	theme	nanoparticle	394:405	arg1	technologies					407:418	nanoparticle technologies	394:418	nanoparticle technologies	394:418	Its clinical use would be promoted by novel functionalization of C60 with the aid of drug delivery carriers based on nanoparticle technologies.
29131597	2	57	theme	delivery	367:374	arg1	carriers					376:383	drug delivery carriers	362:383	drug delivery carriers based on nanoparticle technologies	362:418	Its clinical use would be promoted by novel functionalization of C60 with the aid of drug delivery carriers based on nanoparticle technologies.
29131597	1	58	theme	biomedical	252:261	arg1	applications					263:274	its biomedical applications	248:274	its biomedical applications	248:274	Buckminsterfullerene (C60), known for its strong radical scavenging properties, has been studied extensively for its biomedical applications.
25392347	9	0	theme	genus	937:941	arg1	Gilvimarinus					943:954	the genus Gilvimarinus	933:954	the genus Gilvimarinus	933:954	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YN3(T) should be assigned to the genus Gilvimarinus.
25392347	5	1	theme	respiratory	523:533	arg1	quinone					535:541	The predominant respiratory quinone	507:541	The predominant respiratory quinone	507:541	The predominant respiratory quinone was ubiquinone-8.
25392347	5	1	theme	respiratory	523:533	arg1	ubiquinone-8					547:558	ubiquinone-8	547:558	ubiquinone-8	547:558	The predominant respiratory quinone was ubiquinone-8.
25392347	8	2	theme	mol	821:823	arg1	content					804:810	The genomic DNA G+C content	784:810	The genomic DNA G+C content	784:810	The genomic DNA G+C content was 49.4 mol%.
25392347	8	2	theme	mol	821:823	arg1	%					824:824	49.4 mol%	816:824	49.4 mol%	816:824	The genomic DNA G+C content was 49.4 mol%.
25392347	10	3	theme	closest	1039:1045	arg1	relationship					1060:1071	the closest phylogenetic relationship	1035:1071	the closest phylogenetic relationship to strain YN3(T)	1035:1088	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	11	4	theme	phenotypic	1173:1182	arg1	data					1214:1217	phenotypic, chemotaxonomic and genotypic data	1173:1217	phenotypic, chemotaxonomic and genotypic data	1173:1217	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	11	5	theme	genus	1318:1322	arg1	Gilvimarinus					1324:1335	the genus Gilvimarinus	1314:1335	the genus Gilvimarinus	1314:1335	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	4	6	theme	polysaccharides	458:472	arg1	hydrolysis					444:453	the hydrolysis	440:453	the hydrolysis of polysaccharides, such as agar, starch and xylan	440:504	Strain YN3(T) was positive for the hydrolysis of polysaccharides, such as agar, starch and xylan.
25392347	13	7	theme	T	1453:1453	arg1	19198					1462:1466	 = KCTC 32438(T) = JCM 19198	1439:1466	 = KCTC 32438(T) = JCM 19198(T)	1439:1469	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	7	theme	T	1453:1453	arg1	YN3					1431:1433	YN3	1431:1433	YN3(T) ( = KCTC 32438(T) = JCM 19198(T))	1431:1470	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	7	theme	T	1453:1453	arg1	T					1468:1468	T	1468:1468	T	1468:1468	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	11	8	theme	strain	1270:1275	arg1	T					1281:1281	T	1281:1281	T	1281:1281	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	11	8	theme	strain	1270:1275	arg1	YN3					1277:1279	strain YN3	1270:1279	strain YN3(T)	1270:1282	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	13	9	theme	type	1416:1419	arg1	YN3					1431:1433	YN3	1431:1433	YN3(T) ( = KCTC 32438(T) = JCM 19198(T))	1431:1470	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	9	theme	type	1416:1419	arg1	strain					1421:1426	The type strain	1412:1426	The type strain	1412:1426	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	10	theme	KCTC	1442:1445	arg1	19198					1462:1466	 = KCTC 32438(T) = JCM 19198	1439:1466	 = KCTC 32438(T) = JCM 19198(T)	1439:1469	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	10	theme	KCTC	1442:1445	arg1	YN3					1431:1433	YN3	1431:1433	YN3(T) ( = KCTC 32438(T) = JCM 19198(T))	1431:1470	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	10	theme	KCTC	1442:1445	arg1	T					1468:1468	T	1468:1468	T	1468:1468	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	10	11	dep	%	1107:1107	arg1	similarities					1129:1140	sequence similarities	1120:1140	sequence similarities	1120:1140	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	1	12	theme	Gilvimarinus	131:142	arg1	description					106:116	seaweed, and emended description	85:116	seaweed, and emended description of the genus Gilvimarinus	85:142	nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus.
25392347	10	13	theme	phylogenetic	1047:1058	arg1	relationship					1060:1071	the closest phylogenetic relationship	1035:1071	the closest phylogenetic relationship to strain YN3(T)	1035:1088	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	2	14	theme	taxonomic	147:155	arg1	study					157:161	A taxonomic study	145:161	A taxonomic study	145:161	A taxonomic study was carried out on strain YN3(T), which was isolated from a seaweed sample taken from the coast of Weihai, China.
25392347	11	15	theme	genotypic	1204:1212	arg1	data					1214:1217	phenotypic, chemotaxonomic and genotypic data	1173:1217	phenotypic, chemotaxonomic and genotypic data	1173:1217	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	1	16	theme	seaweed	85:91	arg1	description					106:116	seaweed, and emended description	85:116	seaweed, and emended description of the genus Gilvimarinus	85:142	nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus.
25392347	11	17	theme	DNA-DNA	1223:1229	arg1	studies					1245:1251	DNA-DNA hybridization studies	1223:1251	DNA-DNA hybridization studies	1223:1251	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	0	18	theme	polysaccharolyticus	13:31	arg1	sp					33:34	Gilvimarinus polysaccharolyticus sp	0:34	Gilvimarinus polysaccharolyticus sp.	0:35	Gilvimarinus polysaccharolyticus sp.
25392347	13	19	theme	 =	1439:1440	arg1	19198					1462:1466	 = KCTC 32438(T) = JCM 19198	1439:1466	 = KCTC 32438(T) = JCM 19198(T)	1439:1469	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	19	theme	 =	1439:1440	arg1	YN3					1431:1433	YN3	1431:1433	YN3(T) ( = KCTC 32438(T) = JCM 19198(T))	1431:1470	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	19	theme	 =	1439:1440	arg1	T					1468:1468	T	1468:1468	T	1468:1468	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	10	20	contain	had	1031:1033	arg1	23325					990:994	'Gilvimarinus agarilyticus' KCTC 23325	957:994	'Gilvimarinus agarilyticus' KCTC 23325	957:994	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	10	20	contain	had	1031:1033	arg1	chinensis					1013:1021	Gilvimarinus chinensis QM42	1000:1026	Gilvimarinus chinensis QM42(T)	1000:1029	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	10	20	contain	had	1031:1033	arg1	T					1028:1028	T	1028:1028	T	1028:1028	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	10	20	contain	had	1031:1033	arg2	relationship					1060:1071	the closest phylogenetic relationship	1035:1071	the closest phylogenetic relationship to strain YN3(T)	1035:1088	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	11	21	theme	hybridization	1231:1243	arg1	studies					1245:1251	DNA-DNA hybridization studies	1223:1251	DNA-DNA hybridization studies	1223:1251	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	3	22	theme	4-32	362:365	arg1	°C					367:368	4-32 °C	362:368	4-32 °C	362:368	The bacterium was Gram-stain-negative, rod-shaped, and could grow at pH 5.0-10.0 and 4-32 °C in the presence of 0-9.0 % (w/v) NaCl.
25392347	0	23	theme	Gilvimarinus	0:11	arg1	sp					33:34	Gilvimarinus polysaccharolyticus sp	0:34	Gilvimarinus polysaccharolyticus sp.	0:35	Gilvimarinus polysaccharolyticus sp.
25392347	1	24	attach	isolated	71:78	arg2	bacterium					61:69	an agar-digesting bacterium	43:69	an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus	43:142	nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus.
25392347	1	24	attach	isolated	71:78	arg2	nov.					37:40	nov.	37:40	nov.	37:40	nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus.
25392347	1	24	attach	isolated	71:78	arg1	description					106:116	seaweed, and emended description	85:116	seaweed, and emended description of the genus Gilvimarinus	85:142	nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus.
25392347	5	25	theme	predominant	511:521	arg1	quinone					535:541	The predominant respiratory quinone	507:541	The predominant respiratory quinone	507:541	The predominant respiratory quinone was ubiquinone-8.
25392347	5	25	theme	predominant	511:521	arg1	ubiquinone-8					547:558	ubiquinone-8	547:558	ubiquinone-8	547:558	The predominant respiratory quinone was ubiquinone-8.
25392347	6	26	theme	fatty	571:575	arg1	C16					588:590	C16	588:590	C16	588:590	The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c.
25392347	6	26	theme	fatty	571:575	arg1	acids					577:581	The major fatty acids	561:581	The major fatty acids	561:581	The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c.
25392347	10	27	theme	agarilyticus	971:982	arg1	23325					990:994	'Gilvimarinus agarilyticus' KCTC 23325	957:994	'Gilvimarinus agarilyticus' KCTC 23325	957:994	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	6	28	dep	C16	588:590	arg1	C18					636:638	C18	636:638	C18	636:638	The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c.
25392347	6	28	dep	C16	588:590	arg1	C16					624:626	C16	624:626	C16	624:626	The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c.
25392347	6	28	dep	C16	588:590	arg1	1ω7c					642:645	1ω7c	642:645	C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c	588:645	The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c.
25392347	6	28	dep	C16	588:590	arg1	1ω7c					594:597	1ω7c	594:597	1ω7c	594:597	The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c.
25392347	6	28	dep	C16	588:590	arg1	iso-C15					606:612	iso-C15	606:612	iso-C15	606:612	The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c.
25392347	6	28	dep	C16	588:590	arg1	0					630:630	0	630:630	0	630:630	The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c.
25392347	1	29	theme	emended	98:104	arg1	description					106:116	seaweed, and emended description	85:116	seaweed, and emended description of the genus Gilvimarinus	85:142	nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus.
25392347	6	30	theme	major	565:569	arg1	C16					588:590	C16	588:590	C16	588:590	The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c.
25392347	6	30	theme	major	565:569	arg1	acids					577:581	The major fatty acids	561:581	The major fatty acids	561:581	The major fatty acids were C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c.
25392347	3	31	theme	0-9.0	389:393	arg1	%					395:395	%	395:395	%	395:395	The bacterium was Gram-stain-negative, rod-shaped, and could grow at pH 5.0-10.0 and 4-32 °C in the presence of 0-9.0 % (w/v) NaCl.
25392347	14	32	theme	genus	1503:1507	arg1	Gilvimarinus					1509:1520	the genus Gilvimarinus	1499:1520	the genus Gilvimarinus	1499:1520	An emended description of the genus Gilvimarinus is also presented.
25392347	7	33	theme	main	652:655	arg1	lipids					663:668	The main polar lipids	648:668	The main polar lipids	648:668	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and two unidentified glycolipids.
25392347	7	33	theme	main	652:655	arg1	diphosphatidylglycerol					675:696	diphosphatidylglycerol	675:696	diphosphatidylglycerol	675:696	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and two unidentified glycolipids.
25392347	3	34	theme	%	395:395	arg1	NaCl					403:406	0-9.0 % (w/v) NaCl	389:406	0-9.0 % (w/v) NaCl	389:406	The bacterium was Gram-stain-negative, rod-shaped, and could grow at pH 5.0-10.0 and 4-32 °C in the presence of 0-9.0 % (w/v) NaCl.
25392347	13	35	theme	 =	1455:1456	arg1	19198					1462:1466	 = KCTC 32438(T) = JCM 19198	1439:1466	 = KCTC 32438(T) = JCM 19198(T)	1439:1469	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	35	theme	 =	1455:1456	arg1	YN3					1431:1433	YN3	1431:1433	YN3(T) ( = KCTC 32438(T) = JCM 19198(T))	1431:1470	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	35	theme	 =	1455:1456	arg1	T					1468:1468	T	1468:1468	T	1468:1468	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	11	36	theme	Gilvimarinus	1357:1368	arg1	sp					1390:1391	the name Gilvimarinus polysaccharolyticus sp	1348:1391	the name Gilvimarinus polysaccharolyticus sp	1348:1391	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	7	37	theme	polar	657:661	arg1	lipids					663:668	The main polar lipids	648:668	The main polar lipids	648:668	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and two unidentified glycolipids.
25392347	7	37	theme	polar	657:661	arg1	diphosphatidylglycerol					675:696	diphosphatidylglycerol	675:696	diphosphatidylglycerol	675:696	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and two unidentified glycolipids.
25392347	11	38	theme	Gilvimarinus	1324:1335	arg1	species					1303:1309	a novel species	1295:1309	a novel species	1295:1309	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	9	39	theme	16S	858:860	arg1	sequences					872:880	16S rRNA gene sequences	858:880	16S rRNA gene sequences	858:880	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YN3(T) should be assigned to the genus Gilvimarinus.
25392347	10	40	theme	Gilvimarinus	1000:1011	arg1	chinensis					1013:1021	Gilvimarinus chinensis QM42	1000:1026	Gilvimarinus chinensis QM42(T)	1000:1029	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	10	40	theme	Gilvimarinus	1000:1011	arg1	T					1028:1028	T	1028:1028	T	1028:1028	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	13	41	theme	JCM	1458:1460	arg1	19198					1462:1466	 = KCTC 32438(T) = JCM 19198	1439:1466	 = KCTC 32438(T) = JCM 19198(T)	1439:1469	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	41	theme	JCM	1458:1460	arg1	YN3					1431:1433	YN3	1431:1433	YN3(T) ( = KCTC 32438(T) = JCM 19198(T))	1431:1470	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	41	theme	JCM	1458:1460	arg1	T					1468:1468	T	1468:1468	T	1468:1468	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	8	42	theme	DNA	796:798	arg1	content					804:810	The genomic DNA G+C content	784:810	The genomic DNA G+C content	784:810	The genomic DNA G+C content was 49.4 mol%.
25392347	8	42	theme	DNA	796:798	arg1	%					824:824	49.4 mol%	816:824	49.4 mol%	816:824	The genomic DNA G+C content was 49.4 mol%.
25392347	10	43	theme	KCTC	985:988	arg1	23325					990:994	'Gilvimarinus agarilyticus' KCTC 23325	957:994	'Gilvimarinus agarilyticus' KCTC 23325	957:994	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	9	44	theme	rRNA	862:865	arg1	sequences					872:880	16S rRNA gene sequences	858:880	16S rRNA gene sequences	858:880	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YN3(T) should be assigned to the genus Gilvimarinus.
25392347	13	45	theme	32438	1447:1451	arg1	19198					1462:1466	 = KCTC 32438(T) = JCM 19198	1439:1466	 = KCTC 32438(T) = JCM 19198(T)	1439:1469	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	45	theme	32438	1447:1451	arg1	YN3					1431:1433	YN3	1431:1433	YN3(T) ( = KCTC 32438(T) = JCM 19198(T))	1431:1470	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	13	45	theme	32438	1447:1451	arg1	T					1468:1468	T	1468:1468	T	1468:1468	The type strain is YN3(T) ( = KCTC 32438(T) = JCM 19198(T)).
25392347	14	46	theme	Gilvimarinus	1509:1520	arg1	description					1484:1494	An emended description	1473:1494	An emended description of the genus Gilvimarinus	1473:1520	An emended description of the genus Gilvimarinus is also presented.
25392347	8	47	theme	genomic	788:794	arg1	content					804:810	The genomic DNA G+C content	784:810	The genomic DNA G+C content	784:810	The genomic DNA G+C content was 49.4 mol%.
25392347	8	47	theme	genomic	788:794	arg1	%					824:824	49.4 mol%	816:824	49.4 mol%	816:824	The genomic DNA G+C content was 49.4 mol%.
25392347	9	48	theme	gene	867:870	arg1	sequences					872:880	16S rRNA gene sequences	858:880	16S rRNA gene sequences	858:880	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YN3(T) should be assigned to the genus Gilvimarinus.
25392347	14	49	theme	emended	1476:1482	arg1	description					1484:1494	An emended description	1473:1494	An emended description of the genus Gilvimarinus	1473:1520	An emended description of the genus Gilvimarinus is also presented.
25392347	3	50	dep	Gram-stain-negative	295:313	arg1	Gram-stain-negative					295:313	Gram-stain-negative	295:313	Gram-stain-negative	295:313	The bacterium was Gram-stain-negative, rod-shaped, and could grow at pH 5.0-10.0 and 4-32 °C in the presence of 0-9.0 % (w/v) NaCl.
25392347	3	50	dep	Gram-stain-negative	295:313	arg1	bacterium					281:289	The bacterium	277:289	The bacterium	277:289	The bacterium was Gram-stain-negative, rod-shaped, and could grow at pH 5.0-10.0 and 4-32 °C in the presence of 0-9.0 % (w/v) NaCl.
25392347	3	50	dep	Gram-stain-negative	295:313	arg1	rod-shaped					316:325	rod-shaped	316:325	rod-shaped	316:325	The bacterium was Gram-stain-negative, rod-shaped, and could grow at pH 5.0-10.0 and 4-32 °C in the presence of 0-9.0 % (w/v) NaCl.
25392347	11	51	theme	name	1352:1355	arg1	sp					1390:1391	the name Gilvimarinus polysaccharolyticus sp	1348:1391	the name Gilvimarinus polysaccharolyticus sp	1348:1391	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	3	52	theme	NaCl	403:406	arg1	presence					377:384	the presence	373:384	the presence of 0-9.0 % (w/v) NaCl	373:406	The bacterium was Gram-stain-negative, rod-shaped, and could grow at pH 5.0-10.0 and 4-32 °C in the presence of 0-9.0 % (w/v) NaCl.
25392347	10	53	theme	strain	1076:1081	arg1	T					1087:1087	T	1087:1087	T	1087:1087	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	10	53	theme	strain	1076:1081	arg1	YN3					1083:1085	strain YN3	1076:1085	strain YN3(T)	1076:1088	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	1	54	theme	agar-digesting	46:59	arg1	bacterium					61:69	an agar-digesting bacterium	43:69	an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus	43:142	nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus.
25392347	1	54	theme	agar-digesting	46:59	arg1	nov.					37:40	nov.	37:40	nov.	37:40	nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus.
25392347	11	55	dep	data	1214:1217	arg1	the					1160:1162	the	1160:1162	the	1160:1162	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	11	55	dep	data	1214:1217	arg1	basis					1164:1168	basis	1164:1168	basis	1164:1168	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	2	56	theme	seaweed	223:229	arg1	sample					231:236	a seaweed sample	221:236	a seaweed sample taken from the coast of Weihai, China	221:274	A taxonomic study was carried out on strain YN3(T), which was isolated from a seaweed sample taken from the coast of Weihai, China.
25392347	8	57	theme	G+C	800:802	arg1	content					804:810	The genomic DNA G+C content	784:810	The genomic DNA G+C content	784:810	The genomic DNA G+C content was 49.4 mol%.
25392347	8	57	theme	G+C	800:802	arg1	%					824:824	49.4 mol%	816:824	49.4 mol%	816:824	The genomic DNA G+C content was 49.4 mol%.
25392347	1	58	theme	genus	125:129	arg1	Gilvimarinus					131:142	the genus Gilvimarinus	121:142	the genus Gilvimarinus	121:142	nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus.
25392347	10	59	theme	sequence	1120:1127	arg1	similarities					1129:1140	sequence similarities	1120:1140	sequence similarities	1120:1140	'Gilvimarinus agarilyticus' KCTC 23325 and Gilvimarinus chinensis QM42(T) had the closest phylogenetic relationship to strain YN3(T), and showed 97.9 % and 95.8 % sequence similarities, respectively.
25392347	11	60	theme	polysaccharolyticus	1370:1388	arg1	sp					1390:1391	the name Gilvimarinus polysaccharolyticus sp	1348:1391	the name Gilvimarinus polysaccharolyticus sp	1348:1391	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	2	61	theme	China	270:274	arg1	coast					253:257	the coast	249:257	the coast of Weihai, China	249:274	A taxonomic study was carried out on strain YN3(T), which was isolated from a seaweed sample taken from the coast of Weihai, China.
25392347	7	62	theme	unidentified	758:769	arg1	glycolipids					771:781	two unidentified glycolipids	754:781	two unidentified glycolipids	754:781	The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and two unidentified glycolipids.
25392347	2	63	theme	strain	182:187	arg1	YN3					189:191	strain YN3	182:191	strain YN3(T)	182:194	A taxonomic study was carried out on strain YN3(T), which was isolated from a seaweed sample taken from the coast of Weihai, China.
25392347	2	63	theme	strain	182:187	arg1	T					193:193	T	193:193	T	193:193	A taxonomic study was carried out on strain YN3(T), which was isolated from a seaweed sample taken from the coast of Weihai, China.
25392347	9	64	theme	Phylogenetic	827:838	arg1	analysis					840:847	Phylogenetic analysis	827:847	Phylogenetic analysis based on 16S rRNA gene sequences	827:880	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YN3(T) should be assigned to the genus Gilvimarinus.
25392347	9	65	theme	strain	897:902	arg1	T					908:908	T	908:908	T	908:908	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YN3(T) should be assigned to the genus Gilvimarinus.
25392347	9	65	theme	strain	897:902	arg1	YN3					904:906	strain YN3	897:906	strain YN3(T)	897:909	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YN3(T) should be assigned to the genus Gilvimarinus.
25392347	2	66	attach	isolated	207:214	arg2	T					193:193	T	193:193	T	193:193	A taxonomic study was carried out on strain YN3(T), which was isolated from a seaweed sample taken from the coast of Weihai, China.
25392347	2	66	attach	isolated	207:214	arg2	YN3					189:191	strain YN3	182:191	strain YN3(T)	182:194	A taxonomic study was carried out on strain YN3(T), which was isolated from a seaweed sample taken from the coast of Weihai, China.
25392347	2	66	attach	isolated	207:214	arg1	sample					231:236	a seaweed sample	221:236	a seaweed sample taken from the coast of Weihai, China	221:274	A taxonomic study was carried out on strain YN3(T), which was isolated from a seaweed sample taken from the coast of Weihai, China.
25392347	11	67	theme	chemotaxonomic	1185:1198	arg1	data					1214:1217	phenotypic, chemotaxonomic and genotypic data	1173:1217	phenotypic, chemotaxonomic and genotypic data	1173:1217	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
25392347	4	68	theme	Strain	409:414	arg1	T					420:420	T	420:420	T	420:420	Strain YN3(T) was positive for the hydrolysis of polysaccharides, such as agar, starch and xylan.
25392347	4	68	theme	Strain	409:414	arg1	YN3					416:418	Strain YN3	409:418	Strain YN3(T)	409:421	Strain YN3(T) was positive for the hydrolysis of polysaccharides, such as agar, starch and xylan.
25392347	3	69	dep	%	395:395	arg1	w/v					398:400	w/v	398:400	w/v	398:400	The bacterium was Gram-stain-negative, rod-shaped, and could grow at pH 5.0-10.0 and 4-32 °C in the presence of 0-9.0 % (w/v) NaCl.
25392347	11	70	theme	novel	1297:1301	arg1	species					1303:1309	a novel species	1295:1309	a novel species	1295:1309	On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3(T) represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp.
28708608	0	0	theme	consummate	64:73	arg1	sorption					75:82	consummate sorption	64:82	consummate sorption of chromate	64:94	Magnetic Schiff's base sorbent based on shrimp peels wastes for consummate sorption of chromate.
28708608	3	1	theme	Chitosan	323:330	arg1	process					345:351	Chitosan modification process	323:351	Chitosan modification process	323:351	Chitosan modification process took place through the reaction between chitosan and polymeric Schiff's base of thiourea/glutaraldehyde in the presence of magnetite.
28708608	6	2	theme	%	942:942	arg1	efficiency					987:996	99.1% (sorption capacity; 252.45 mg g-1) removal efficiency	938:996	99.1% (sorption capacity; 252.45 mg g-1) removal efficiency	938:996	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	3	3	theme	modification	332:343	arg1	process					345:351	Chitosan modification process	323:351	Chitosan modification process	323:351	Chitosan modification process took place through the reaction between chitosan and polymeric Schiff's base of thiourea/glutaraldehyde in the presence of magnetite.
28708608	4	4	dep	spectroscopy	566:577	arg1	analyses					636:643	dispersive X-ray analyses	619:643	dispersive X-ray analyses	619:643	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	3	5	dep	chitosan	393:400	arg1	base					425:428	Schiff's base	416:428	Schiff's base	416:428	Chitosan modification process took place through the reaction between chitosan and polymeric Schiff's base of thiourea/glutaraldehyde in the presence of magnetite.
28708608	5	6	theme	batch	773:777	arg1	experiments					788:798	batch sorption experiments	773:798	batch sorption experiments	773:798	The performance of the sorbent was systematically evaluated by batch sorption experiments, followed by equilibrium and kinetic studies with different mathematical models.
28708608	7	7	theme	isotherm	1159:1166	arg1	model					1168:1172	Langmuir isotherm model	1150:1172	Langmuir isotherm model	1150:1172	Experimental data gave better mathematical fitting towards pseudo-second-order kinetic model and Langmuir isotherm model.
28708608	2	8	theme	demineralization	270:285	arg1	deproteinization					287:302	demineralization deproteinization	270:302	demineralization deproteinization	270:302	Chitosan extraction involved three main stages as preconditioning, demineralization deproteinization and deacetylation.
28708608	5	9	theme	sorption	779:786	arg1	experiments					788:798	batch sorption experiments	773:798	batch sorption experiments	773:798	The performance of the sorbent was systematically evaluated by batch sorption experiments, followed by equilibrium and kinetic studies with different mathematical models.
28708608	4	10	theme	hybrid	501:506	arg1	composite					508:516	The synthetic hybrid composite	487:516	The synthetic hybrid composite	487:516	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	6	11	theme	removal	979:985	arg1	efficiency					987:996	99.1% (sorption capacity; 252.45 mg g-1) removal efficiency	938:996	99.1% (sorption capacity; 252.45 mg g-1) removal efficiency	938:996	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	4	12	theme	synthetic	491:499	arg1	composite					508:516	The synthetic hybrid composite	487:516	The synthetic hybrid composite	487:516	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	0	13	theme	chromate	87:94	arg1	sorption					75:82	consummate sorption	64:82	consummate sorption of chromate	64:94	Magnetic Schiff's base sorbent based on shrimp peels wastes for consummate sorption of chromate.
28708608	7	14	theme	Langmuir	1150:1157	arg1	model					1168:1172	Langmuir isotherm model	1150:1172	Langmuir isotherm model	1150:1172	Experimental data gave better mathematical fitting towards pseudo-second-order kinetic model and Langmuir isotherm model.
28708608	5	15	with	studies	837:843	arg1	models					873:878	different mathematical models	850:878	different mathematical models	850:878	The performance of the sorbent was systematically evaluated by batch sorption experiments, followed by equilibrium and kinetic studies with different mathematical models.
28708608	4	16	from	solution	700:707	arg1	Cr					671:672	Cr(VI) recovery from aqueous solution	671:707	Cr(VI) recovery from aqueous solution	671:707	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	4	17	theme	aqueous	692:698	arg1	solution					700:707	aqueous solution	692:707	aqueous solution	692:707	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	5	18	theme	mathematical	860:871	arg1	models					873:878	different mathematical models	850:878	different mathematical models	850:878	The performance of the sorbent was systematically evaluated by batch sorption experiments, followed by equilibrium and kinetic studies with different mathematical models.
28708608	6	19	theme	Cr	1001:1002	arg1	solution					1008:1015	Cr(VI) solution	1001:1015	Cr(VI) solution	1001:1015	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	7	20	theme	kinetic	1132:1138	arg1	model					1140:1144	pseudo-second-order kinetic model	1112:1144	pseudo-second-order kinetic model	1112:1144	Experimental data gave better mathematical fitting towards pseudo-second-order kinetic model and Langmuir isotherm model.
28708608	6	21	theme	sorption	945:952	arg1	capacity					954:961	sorption capacity	945:961	sorption capacity; 252.45 mg g-1	945:976	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	3	22	theme	thiourea/glutaraldehyde	433:455	arg1	chitosan					393:400	chitosan	393:400	chitosan	393:400	Chitosan modification process took place through the reaction between chitosan and polymeric Schiff's base of thiourea/glutaraldehyde in the presence of magnetite.
28708608	0	23	theme	Magnetic	0:7	arg1	sorbent					23:29	Magnetic Schiff's base sorbent	0:29	Magnetic Schiff's base sorbent based on shrimp peels	0:51	Magnetic Schiff's base sorbent based on shrimp peels wastes for consummate sorption of chromate.
28708608	6	24	theme	isotherm	885:892	arg1	study					894:898	The isotherm study	881:898	The isotherm study	881:898	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	8	25	dep	endothermic	1314:1324	arg1	endothermic					1314:1324	endothermic	1314:1324	endothermic	1314:1324	The distribution coefficient was obtained at different temperatures and the thermodynamic parameters have been calculated: the sorption is endothermic, spontaneous and contributes to increase the randomness of the system.
28708608	8	25	dep	endothermic	1314:1324	arg1	sorption					1302:1309	the sorption	1298:1309	the sorption	1298:1309	The distribution coefficient was obtained at different temperatures and the thermodynamic parameters have been calculated: the sorption is endothermic, spontaneous and contributes to increase the randomness of the system.
28708608	8	25	dep	endothermic	1314:1324	arg1	spontaneous					1327:1337	spontaneous	1327:1337	spontaneous	1327:1337	The distribution coefficient was obtained at different temperatures and the thermodynamic parameters have been calculated: the sorption is endothermic, spontaneous and contributes to increase the randomness of the system.
28708608	1	26	theme	shrimp	171:176	arg1	peels					178:182	shrimp peels	171:182	shrimp peels as a raw material	171:200	Magnetic Schiff's base chitosan composite has been prepared starting from shrimp peels as a raw material.
28708608	0	27	theme	base	18:21	arg1	sorbent					23:29	Magnetic Schiff's base sorbent	0:29	Magnetic Schiff's base sorbent based on shrimp peels	0:51	Magnetic Schiff's base sorbent based on shrimp peels wastes for consummate sorption of chromate.
28708608	4	28	theme	electron	592:599	arg1	microscopy-energy					601:617	scanning electron microscopy-energy	583:617	scanning electron microscopy-energy	583:617	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	4	29	theme	X-ray	630:634	arg1	analyses					636:643	dispersive X-ray analyses	619:643	dispersive X-ray analyses	619:643	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	7	30	theme	pseudo-second-order	1112:1130	arg1	model					1140:1144	pseudo-second-order kinetic model	1112:1144	pseudo-second-order kinetic model	1112:1144	Experimental data gave better mathematical fitting towards pseudo-second-order kinetic model and Langmuir isotherm model.
28708608	6	31	theme	99.1	938:941	arg1	%					942:942	%	942:942	%	942:942	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	6	32	theme	mg	971:972	arg1	g-1					974:976	252.45 mg g-1	964:976	sorption capacity; 252.45 mg g-1	945:976	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	6	33	dep	mg/L	1047:1050	arg1	up					1037:1038	up	1037:1038	up	1037:1038	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	5	34	theme	kinetic	829:835	arg1	studies					837:843	kinetic studies	829:843	kinetic studies	829:843	The performance of the sorbent was systematically evaluated by batch sorption experiments, followed by equilibrium and kinetic studies with different mathematical models.
28708608	4	35	theme	dispersive	619:628	arg1	analyses					636:643	dispersive X-ray analyses	619:643	dispersive X-ray analyses	619:643	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	7	36	theme	mathematical	1083:1094	arg1	fitting					1096:1102	better mathematical fitting	1076:1102	better mathematical fitting	1076:1102	Experimental data gave better mathematical fitting towards pseudo-second-order kinetic model and Langmuir isotherm model.
28708608	8	37	theme	system	1389:1394	arg1	randomness					1371:1380	the randomness	1367:1380	the randomness of the system	1367:1394	The distribution coefficient was obtained at different temperatures and the thermodynamic parameters have been calculated: the sorption is endothermic, spontaneous and contributes to increase the randomness of the system.
28708608	6	38	dep	solution	1008:1015	arg1	VI					1004:1005	VI	1004:1005	VI	1004:1005	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	4	39	dep	transform	547:555	arg1	tested					649:654	tested	649:654	tested as sorbent for Cr(VI) recovery from aqueous solution	649:707	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	4	39	dep	transform	547:555	arg1	infrared					557:564	infrared	557:564	infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses	557:643	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	2	40	theme	Chitosan	203:210	arg1	extraction					212:221	Chitosan extraction	203:221	Chitosan extraction	203:221	Chitosan extraction involved three main stages as preconditioning, demineralization deproteinization and deacetylation.
28708608	3	41	theme	magnetite	476:484	arg1	presence					464:471	the presence	460:471	the presence of magnetite	460:484	Chitosan modification process took place through the reaction between chitosan and polymeric Schiff's base of thiourea/glutaraldehyde in the presence of magnetite.
28708608	0	42	theme	shrimp	40:45	arg1	peels					47:51	shrimp peels	40:51	shrimp peels	40:51	Magnetic Schiff's base sorbent based on shrimp peels wastes for consummate sorption of chromate.
28708608	4	43	dep	Fourier	539:545	arg1	transform					547:555	transform	547:555	transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution	547:707	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	1	44	theme	Magnetic	97:104	arg1	composite					129:137	Magnetic Schiff's base chitosan composite	97:137	Magnetic Schiff's base chitosan composite	97:137	Magnetic Schiff's base chitosan composite has been prepared starting from shrimp peels as a raw material.
28708608	5	45	theme	different	850:858	arg1	models					873:878	different mathematical models	850:878	different mathematical models	850:878	The performance of the sorbent was systematically evaluated by batch sorption experiments, followed by equilibrium and kinetic studies with different mathematical models.
28708608	4	46	theme	scanning	583:590	arg1	microscopy-energy					601:617	scanning electron microscopy-energy	583:617	scanning electron microscopy-energy	583:617	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	7	47	theme	better	1076:1081	arg1	fitting					1096:1102	better mathematical fitting	1076:1102	better mathematical fitting	1076:1102	Experimental data gave better mathematical fitting towards pseudo-second-order kinetic model and Langmuir isotherm model.
28708608	6	48	theme	252.45	964:969	arg1	mg					971:972	mg	971:972	mg	971:972	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	7	49	theme	Experimental	1053:1064	arg1	data					1066:1069	Experimental data	1053:1069	Experimental data	1053:1069	Experimental data gave better mathematical fitting towards pseudo-second-order kinetic model and Langmuir isotherm model.
28708608	4	50	dep	Cr	671:672	arg1	recovery					678:685	recovery	678:685	Cr(VI) recovery from aqueous solution	671:707	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	4	50	dep	Cr	671:672	arg1	VI					674:675	VI	674:675	VI	674:675	The synthetic hybrid composite was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy-energy dispersive X-ray analyses and tested as sorbent for Cr(VI) recovery from aqueous solution.
28708608	5	51	with	equilibrium	813:823	arg1	models					873:878	different mathematical models	850:878	different mathematical models	850:878	The performance of the sorbent was systematically evaluated by batch sorption experiments, followed by equilibrium and kinetic studies with different mathematical models.
28708608	9	52	theme	sorption/desorption	1447:1465	arg1	cycles					1437:1442	three cycles	1431:1442	three cycles of sorption/desorption	1431:1465	The sorbent could be recycled for three cycles of sorption/desorption.
28708608	8	53	theme	different	1220:1228	arg1	temperatures					1230:1241	different temperatures	1220:1241	different temperatures	1220:1241	The distribution coefficient was obtained at different temperatures and the thermodynamic parameters have been calculated: the sorption is endothermic, spontaneous and contributes to increase the randomness of the system.
28708608	5	54	theme	sorbent	733:739	arg1	performance					714:724	The performance	710:724	The performance of the sorbent	710:739	The performance of the sorbent was systematically evaluated by batch sorption experiments, followed by equilibrium and kinetic studies with different mathematical models.
28708608	2	55	theme	main	238:241	arg1	stages					243:248	three main stages	232:248	three main stages	232:248	Chitosan extraction involved three main stages as preconditioning, demineralization deproteinization and deacetylation.
28708608	1	56	theme	base	115:118	arg1	composite					129:137	Magnetic Schiff's base chitosan composite	97:137	Magnetic Schiff's base chitosan composite	97:137	Magnetic Schiff's base chitosan composite has been prepared starting from shrimp peels as a raw material.
28708608	6	57	dep	%	942:942	arg1	capacity					954:961	sorption capacity	945:961	sorption capacity; 252.45 mg g-1	945:976	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	1	58	theme	raw	189:191	arg1	material					193:200	a raw material	187:200	a raw material	187:200	Magnetic Schiff's base chitosan composite has been prepared starting from shrimp peels as a raw material.
28708608	1	59	theme	chitosan	120:127	arg1	composite					129:137	Magnetic Schiff's base chitosan composite	97:137	Magnetic Schiff's base chitosan composite	97:137	Magnetic Schiff's base chitosan composite has been prepared starting from shrimp peels as a raw material.
28708608	6	60	dep	capacity	954:961	arg1	g-1					974:976	252.45 mg g-1	964:976	sorption capacity; 252.45 mg g-1	945:976	The isotherm study demonstrate that the sorbent achieved 99.1% (sorption capacity; 252.45 mg g-1) removal efficiency in Cr(VI) solution with concentrations up to 400 mg/L.
28708608	8	61	theme	distribution	1179:1190	arg1	coefficient					1192:1202	The distribution coefficient	1175:1202	The distribution coefficient	1175:1202	The distribution coefficient was obtained at different temperatures and the thermodynamic parameters have been calculated: the sorption is endothermic, spontaneous and contributes to increase the randomness of the system.
28708608	8	62	theme	thermodynamic	1251:1263	arg1	parameters					1265:1274	the thermodynamic parameters	1247:1274	the thermodynamic parameters	1247:1274	The distribution coefficient was obtained at different temperatures and the thermodynamic parameters have been calculated: the sorption is endothermic, spontaneous and contributes to increase the randomness of the system.
26721729	1	0	with	combination	232:242	arg1	process					270:276	a hot-melt extrusion process	249:276	a hot-melt extrusion process	249:276	Amorphous solid dispersions have for many years been a focus in oral formulations, especially in combination with a hot-melt extrusion process.
26721729	1	1	theme	many	172:175	arg1	years					177:181	many years	172:181	many years	172:181	Amorphous solid dispersions have for many years been a focus in oral formulations, especially in combination with a hot-melt extrusion process.
26721729	5	2	theme	X-ray	699:703	arg1	diffraction					712:722	X-ray powder diffraction	699:722	X-ray powder diffraction	699:722	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	5	2	theme	X-ray	699:703	arg1	spectroscopy					685:696	Vibrational spectroscopy	673:696	Vibrational spectroscopy	673:696	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	1	3	theme	hot-melt	251:258	arg1	process					270:276	a hot-melt extrusion process	249:276	a hot-melt extrusion process	249:276	Amorphous solid dispersions have for many years been a focus in oral formulations, especially in combination with a hot-melt extrusion process.
26721729	0	4	theme	carrier	88:94	arg1	matrix					96:101	a polymer/inorganic carrier matrix	68:101	a polymer/inorganic carrier matrix produced by hot-melt extrusion	68:132	Molecularly designed lipid microdomains for solid dispersions using a polymer/inorganic carrier matrix produced by hot-melt extrusion.
26721729	5	5	theme	hydroxypropylcellulose	838:859	arg1	system					814:819	a system	812:819	a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate	812:891	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	3	6	theme	solid	481:485	arg1	carrier					487:493	the solid carrier	477:493	the solid carrier	477:493	It was intended to adsorb the acidic lipid by specific molecular interactions onto the solid carrier to design disorder in the alkyl chains of the lipid.
26721729	1	7	theme	extrusion	260:268	arg1	process					270:276	a hot-melt extrusion process	249:276	a hot-melt extrusion process	249:276	Amorphous solid dispersions have for many years been a focus in oral formulations, especially in combination with a hot-melt extrusion process.
26721729	5	8	theme	Vibrational	673:683	arg1	diffraction					712:722	X-ray powder diffraction	699:722	X-ray powder diffraction	699:722	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	5	8	theme	Vibrational	673:683	arg1	spectroscopy					685:696	Vibrational spectroscopy	673:696	Vibrational spectroscopy	673:696	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	5	8	theme	Vibrational	673:683	arg1	microscopy					738:747	atomic force microscopy	725:747	atomic force microscopy	725:747	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	4	9	theme	designed	553:560	arg1	microdomains					568:579	Such designed lipid microdomains	548:579	Such designed lipid microdomains (DLM)	548:585	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
26721729	4	9	theme	designed	553:560	arg1	microstructure					609:622	a new microstructure	603:622	a new microstructure	603:622	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
26721729	4	9	theme	designed	553:560	arg1	DLM					582:584	DLM	582:584	DLM	582:584	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
26721729	9	10	theme	oral	1349:1352	arg1	compounds					1354:1362	oral compounds	1349:1362	oral compounds that exhibit both high crystal energy and lipophilicity	1349:1418	The novel delivery system appeared to be especially promising for the formulation of oral compounds that exhibit both high crystal energy and lipophilicity.
26721729	7	11	theme	specific	1132:1139	arg1	compositions					1141:1152	specific compositions	1132:1152	specific compositions	1132:1152	The results indicated that the targeted molecular excipient interactions indeed led to DLMs for specific compositions.
26721729	2	12	theme	fatty	346:350	arg1	carrier					385:391	an inorganic carrier	372:391	an inorganic carrier	372:391	The present work targets a novel approach with a system based on a fatty acid, a polymer and an inorganic carrier.
26721729	2	12	theme	fatty	346:350	arg1	acid					352:355	a fatty acid	344:355	a fatty acid	344:355	The present work targets a novel approach with a system based on a fatty acid, a polymer and an inorganic carrier.
26721729	2	12	theme	fatty	346:350	arg1	polymer					360:366	a polymer	358:366	a polymer	358:366	The present work targets a novel approach with a system based on a fatty acid, a polymer and an inorganic carrier.
26721729	2	13	with	approach	312:319	arg1	system					328:333	a system	326:333	a system based on a fatty acid, a polymer and an inorganic carrier	326:391	The present work targets a novel approach with a system based on a fatty acid, a polymer and an inorganic carrier.
26721729	5	14	theme	aluminum	865:872	arg1	silicate					884:891	aluminum magnesium silicate	865:891	aluminum magnesium silicate	865:891	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	7	15	theme	molecular	1076:1084	arg1	interactions					1096:1107	the targeted molecular excipient interactions	1063:1107	the targeted molecular excipient interactions	1063:1107	The results indicated that the targeted molecular excipient interactions indeed led to DLMs for specific compositions.
26721729	2	16	theme	present	283:289	arg1	work					291:294	The present work	279:294	The present work	279:294	The present work targets a novel approach with a system based on a fatty acid, a polymer and an inorganic carrier.
26721729	5	17	theme	silicate	884:891	arg1	system					814:819	a system	812:819	a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate	812:891	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	4	18	theme	new	605:607	arg1	microdomains					568:579	Such designed lipid microdomains	548:579	Such designed lipid microdomains (DLM)	548:585	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
26721729	4	18	theme	new	605:607	arg1	microstructure					609:622	a new microstructure	603:622	a new microstructure	603:622	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
26721729	0	19	theme	hot-melt	115:122	arg1	extrusion					124:132	hot-melt extrusion	115:132	hot-melt extrusion	115:132	Molecularly designed lipid microdomains for solid dispersions using a polymer/inorganic carrier matrix produced by hot-melt extrusion.
26721729	5	20	theme	force	732:736	arg1	microscopy					738:747	atomic force microscopy	725:747	atomic force microscopy	725:747	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	5	20	theme	force	732:736	arg1	spectroscopy					685:696	Vibrational spectroscopy	673:696	Vibrational spectroscopy	673:696	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	4	21	theme	lipid	562:566	arg1	microdomains					568:579	Such designed lipid microdomains	548:579	Such designed lipid microdomains (DLM)	548:585	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
26721729	4	21	theme	lipid	562:566	arg1	microstructure					609:622	a new microstructure	603:622	a new microstructure	603:622	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
26721729	4	21	theme	lipid	562:566	arg1	DLM					582:584	DLM	582:584	DLM	582:584	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
26721729	8	22	theme	created	1240:1246	arg1	microstructure					1248:1261	the created microstructure	1236:1261	the created microstructure	1236:1261	The different methods provided complementary aspects and important insights into the created microstructure.
26721729	8	23	theme	complementary	1186:1198	arg1	aspects					1200:1206	complementary aspects	1186:1206	complementary aspects	1186:1206	The different methods provided complementary aspects and important insights into the created microstructure.
26721729	5	24	theme	atomic	725:730	arg1	microscopy					738:747	atomic force microscopy	725:747	atomic force microscopy	725:747	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	5	24	theme	atomic	725:730	arg1	spectroscopy					685:696	Vibrational spectroscopy	673:696	Vibrational spectroscopy	673:696	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	5	25	theme	powder	705:710	arg1	diffraction					712:722	X-ray powder diffraction	699:722	X-ray powder diffraction	699:722	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	5	25	theme	powder	705:710	arg1	spectroscopy					685:696	Vibrational spectroscopy	673:696	Vibrational spectroscopy	673:696	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	5	26	theme	electron	760:767	arg1	imaging					781:787	electron microscopic imaging	760:787	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging	673:787	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	6	27	theme	model	940:944	arg1	substance					946:954	a poorly water-soluble model substance	917:954	a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations	917:1033	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	6	27	theme	model	940:944	arg1	difficult					964:972	difficult	964:972	difficult	964:972	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	6	27	theme	model	940:944	arg1	β-carotene					894:903	β-carotene	894:903	β-carotene	894:903	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	7	28	theme	targeted	1067:1074	arg1	interactions					1096:1107	the targeted molecular excipient interactions	1063:1107	the targeted molecular excipient interactions	1063:1107	The results indicated that the targeted molecular excipient interactions indeed led to DLMs for specific compositions.
26721729	5	29	theme	stearic	824:830	arg1	acid					832:835	stearic acid	824:835	stearic acid	824:835	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	5	30	theme	microscopic	769:779	arg1	imaging					781:787	electron microscopic imaging	760:787	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging	673:787	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	6	31	theme	water-soluble	926:938	arg1	substance					946:954	a poorly water-soluble model substance	917:954	a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations	917:1033	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	6	31	theme	water-soluble	926:938	arg1	difficult					964:972	difficult	964:972	difficult	964:972	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	6	31	theme	water-soluble	926:938	arg1	β-carotene					894:903	β-carotene	894:903	β-carotene	894:903	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	2	32	theme	novel	306:310	arg1	approach					312:319	a novel approach	304:319	a novel approach with a system based on a fatty acid, a polymer and an inorganic carrier	304:391	The present work targets a novel approach with a system based on a fatty acid, a polymer and an inorganic carrier.
26721729	1	33	theme	oral	199:202	arg1	formulations					204:215	oral formulations	199:215	oral formulations	199:215	Amorphous solid dispersions have for many years been a focus in oral formulations, especially in combination with a hot-melt extrusion process.
26721729	0	34	theme	lipid	21:25	arg1	microdomains					27:38	lipid microdomains	21:38	lipid microdomains	21:38	Molecularly designed lipid microdomains for solid dispersions using a polymer/inorganic carrier matrix produced by hot-melt extrusion.
26721729	3	35	theme	design	498:503	arg1	disorder					505:512	design disorder	498:512	design disorder	498:512	It was intended to adsorb the acidic lipid by specific molecular interactions onto the solid carrier to design disorder in the alkyl chains of the lipid.
26721729	0	36	theme	solid	44:48	arg1	dispersions					50:60	solid dispersions	44:60	solid dispersions using a polymer/inorganic carrier matrix produced by hot-melt extrusion	44:132	Molecularly designed lipid microdomains for solid dispersions using a polymer/inorganic carrier matrix produced by hot-melt extrusion.
26721729	5	37	theme	magnesium	874:882	arg1	silicate					884:891	aluminum magnesium silicate	865:891	aluminum magnesium silicate	865:891	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	6	38	used	used	909:912	arg2	β-carotene					894:903	β-carotene	894:903	β-carotene	894:903	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	6	38	used	used	909:912	arg2	difficult					964:972	difficult	964:972	difficult	964:972	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	6	38	used	used	909:912	arg2	substance					946:954	a poorly water-soluble model substance	917:954	a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations	917:1033	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	6	39	theme	dispersion	1011:1020	arg1	formulations					1022:1033	conventional solid dispersion formulations	992:1033	conventional solid dispersion formulations	992:1033	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	8	40	theme	important	1212:1220	arg1	insights					1222:1229	important insights	1212:1229	important insights into the created microstructure	1212:1261	The different methods provided complementary aspects and important insights into the created microstructure.
26721729	3	41	theme	acidic	424:429	arg1	lipid					431:435	the acidic lipid	420:435	the acidic lipid by specific molecular interactions onto the solid carrier to design disorder in the alkyl chains of the lipid	420:545	It was intended to adsorb the acidic lipid by specific molecular interactions onto the solid carrier to design disorder in the alkyl chains of the lipid.
26721729	9	42	theme	high	1382:1385	arg1	energy					1395:1400	high crystal energy	1382:1400	high crystal energy	1382:1400	The novel delivery system appeared to be especially promising for the formulation of oral compounds that exhibit both high crystal energy and lipophilicity.
26721729	6	43	theme	solid	1005:1009	arg1	formulations					1022:1033	conventional solid dispersion formulations	992:1033	conventional solid dispersion formulations	992:1033	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	9	44	theme	compounds	1354:1362	arg1	formulation					1334:1344	the formulation	1330:1344	the formulation of oral compounds that exhibit both high crystal energy and lipophilicity	1330:1418	The novel delivery system appeared to be especially promising for the formulation of oral compounds that exhibit both high crystal energy and lipophilicity.
26721729	3	45	theme	lipid	541:545	arg1	chains					527:532	the alkyl chains	517:532	the alkyl chains of the lipid	517:545	It was intended to adsorb the acidic lipid by specific molecular interactions onto the solid carrier to design disorder in the alkyl chains of the lipid.
26721729	4	46	theme	solid	655:659	arg1	dispersion					661:670	a solid dispersion	653:670	a solid dispersion	653:670	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
26721729	9	47	theme	crystal	1387:1393	arg1	energy					1395:1400	high crystal energy	1382:1400	high crystal energy	1382:1400	The novel delivery system appeared to be especially promising for the formulation of oral compounds that exhibit both high crystal energy and lipophilicity.
26721729	9	48	theme	novel	1268:1272	arg1	promising					1316:1324	promising	1316:1324	promising	1316:1324	The novel delivery system appeared to be especially promising for the formulation of oral compounds that exhibit both high crystal energy and lipophilicity.
26721729	9	48	theme	novel	1268:1272	arg1	system					1283:1288	The novel delivery system	1264:1288	The novel delivery system	1264:1288	The novel delivery system appeared to be especially promising for the formulation of oral compounds that exhibit both high crystal energy and lipophilicity.
26721729	7	49	theme	excipient	1086:1094	arg1	interactions					1096:1107	the targeted molecular excipient interactions	1063:1107	the targeted molecular excipient interactions	1063:1107	The results indicated that the targeted molecular excipient interactions indeed led to DLMs for specific compositions.
26721729	3	50	from	lipid	431:435	arg1	chains					527:532	the alkyl chains	517:532	the alkyl chains of the lipid	517:545	It was intended to adsorb the acidic lipid by specific molecular interactions onto the solid carrier to design disorder in the alkyl chains of the lipid.
26721729	1	51	theme	Amorphous	135:143	arg1	focus					190:194	a focus	188:194	a focus in oral formulations	188:215	Amorphous solid dispersions have for many years been a focus in oral formulations, especially in combination with a hot-melt extrusion process.
26721729	1	51	theme	Amorphous	135:143	arg1	dispersions					151:161	Amorphous solid dispersions	135:161	Amorphous solid dispersions	135:161	Amorphous solid dispersions have for many years been a focus in oral formulations, especially in combination with a hot-melt extrusion process.
26721729	9	52	theme	delivery	1274:1281	arg1	promising					1316:1324	promising	1316:1324	promising	1316:1324	The novel delivery system appeared to be especially promising for the formulation of oral compounds that exhibit both high crystal energy and lipophilicity.
26721729	9	52	theme	delivery	1274:1281	arg1	system					1283:1288	The novel delivery system	1264:1288	The novel delivery system	1264:1288	The novel delivery system appeared to be especially promising for the formulation of oral compounds that exhibit both high crystal energy and lipophilicity.
26721729	1	53	theme	solid	145:149	arg1	focus					190:194	a focus	188:194	a focus in oral formulations	188:215	Amorphous solid dispersions have for many years been a focus in oral formulations, especially in combination with a hot-melt extrusion process.
26721729	1	53	theme	solid	145:149	arg1	dispersions					151:161	Amorphous solid dispersions	135:161	Amorphous solid dispersions	135:161	Amorphous solid dispersions have for many years been a focus in oral formulations, especially in combination with a hot-melt extrusion process.
26721729	3	54	theme	alkyl	521:525	arg1	chains					527:532	the alkyl chains	517:532	the alkyl chains of the lipid	517:545	It was intended to adsorb the acidic lipid by specific molecular interactions onto the solid carrier to design disorder in the alkyl chains of the lipid.
26721729	6	55	theme	conventional	992:1003	arg1	formulations					1022:1033	conventional solid dispersion formulations	992:1033	conventional solid dispersion formulations	992:1033	β-carotene was used as a poorly water-soluble model substance that is difficult to formulate with conventional solid dispersion formulations.
26721729	8	56	theme	different	1159:1167	arg1	methods					1169:1175	The different methods	1155:1175	The different methods	1155:1175	The different methods provided complementary aspects and important insights into the created microstructure.
26721729	1	57	from	focus	190:194	arg1	formulations					204:215	oral formulations	199:215	oral formulations	199:215	Amorphous solid dispersions have for many years been a focus in oral formulations, especially in combination with a hot-melt extrusion process.
26721729	1	57	from	focus	190:194	arg1	combination					232:242	combination	232:242	combination with a hot-melt extrusion process	232:276	Amorphous solid dispersions have for many years been a focus in oral formulations, especially in combination with a hot-melt extrusion process.
26721729	3	58	theme	molecular	449:457	arg1	interactions					459:470	specific molecular interactions	440:470	specific molecular interactions onto the solid carrier	440:493	It was intended to adsorb the acidic lipid by specific molecular interactions onto the solid carrier to design disorder in the alkyl chains of the lipid.
26721729	3	59	theme	specific	440:447	arg1	interactions					459:470	specific molecular interactions	440:470	specific molecular interactions onto the solid carrier	440:493	It was intended to adsorb the acidic lipid by specific molecular interactions onto the solid carrier to design disorder in the alkyl chains of the lipid.
26721729	0	60	theme	polymer/inorganic	70:86	arg1	matrix					96:101	a polymer/inorganic carrier matrix	68:101	a polymer/inorganic carrier matrix produced by hot-melt extrusion	68:132	Molecularly designed lipid microdomains for solid dispersions using a polymer/inorganic carrier matrix produced by hot-melt extrusion.
26721729	2	61	theme	inorganic	375:383	arg1	carrier					385:391	an inorganic carrier	372:391	an inorganic carrier	372:391	The present work targets a novel approach with a system based on a fatty acid, a polymer and an inorganic carrier.
26721729	2	61	theme	inorganic	375:383	arg1	acid					352:355	a fatty acid	344:355	a fatty acid	344:355	The present work targets a novel approach with a system based on a fatty acid, a polymer and an inorganic carrier.
26721729	5	62	theme	acid	832:835	arg1	system					814:819	a system	812:819	a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate	812:891	Vibrational spectroscopy, X-ray powder diffraction, atomic force microscopy as well as electron microscopic imaging were employed to study a system of stearic acid, hydroxypropylcellulose and aluminum magnesium silicate.
26721729	4	63	theme	Such	548:551	arg1	microdomains					568:579	Such designed lipid microdomains	548:579	Such designed lipid microdomains (DLM)	548:585	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
26721729	4	63	theme	Such	548:551	arg1	microstructure					609:622	a new microstructure	603:622	a new microstructure	603:622	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
26721729	4	63	theme	Such	548:551	arg1	DLM					582:584	DLM	582:584	DLM	582:584	Such designed lipid microdomains (DLM) were created as a new microstructure to accommodate a compound in a solid dispersion.
24239581	0	0	theme	approach	95:102	arg1	design					74:79	freeze-dried protein formulations--a design	37:79	freeze-dried protein formulations--a design of experiment approach	37:102	Formation of mannitol hemihydrate in freeze-dried protein formulations--a design of experiment approach.
24239581	5	1	theme	full	775:778	arg1	design					796:801	a full factorial mixed design	773:801	a full factorial mixed design	773:801	Additionally, annealing as process parameter was included in a full factorial mixed design.
24239581	3	2	theme	mannitol	493:500	arg1	formation					514:522	mannitol hemihydrate formation	493:522	mannitol hemihydrate formation	493:522	In this study, design of experiments was applied for response surface modelling of mannitol hemihydrate formation.
24239581	1	3	theme	pharmaceutical	242:255	arg1	formulations					257:268	pharmaceutical formulations	242:268	pharmaceutical formulations	242:268	Since the discovery of mannitol hemihydrate, this form of mannitol has been seen as potentially negative with regard to the stability of pharmaceutical formulations.
24239581	0	4	theme	experiment	84:93	arg1	approach					95:102	experiment approach	84:102	experiment approach	84:102	Formation of mannitol hemihydrate in freeze-dried protein formulations--a design of experiment approach.
24239581	3	5	theme	hemihydrate	502:512	arg1	formation					514:522	mannitol hemihydrate formation	493:522	mannitol hemihydrate formation	493:522	In this study, design of experiments was applied for response surface modelling of mannitol hemihydrate formation.
24239581	1	6	theme	formulations	257:268	arg1	stability					229:237	the stability	225:237	the stability of pharmaceutical formulations	225:268	Since the discovery of mannitol hemihydrate, this form of mannitol has been seen as potentially negative with regard to the stability of pharmaceutical formulations.
24239581	5	7	theme	process	739:745	arg1	parameter					747:755	process parameter	739:755	process parameter	739:755	Additionally, annealing as process parameter was included in a full factorial mixed design.
24239581	7	8	theme	quality	1018:1024	arg1	models					1000:1005	models	1000:1005	models of similar quality	1000:1024	Inclusion of the process parameter annealing resulted in models of similar quality.
24239581	2	9	theme	hemihydrate	297:307	arg1	formation					275:283	The formation	271:283	The formation of mannitol hemihydrate	271:307	The formation of mannitol hemihydrate is reported in several case studies; however, no systematic investigation has been performed so far.
24239581	4	10	theme	overall	609:615	arg1	parameters					541:550	The formulation parameters	525:550	The formulation parameters investigated in a composite face-centred design	525:598	The formulation parameters investigated in a composite face-centred design were the overall solid content, protein concentration, protein type and the ratio between mannitol and sucrose.
24239581	4	10	theme	overall	609:615	arg1	content					623:629	the overall solid content	605:629	the overall solid content	605:629	The formulation parameters investigated in a composite face-centred design were the overall solid content, protein concentration, protein type and the ratio between mannitol and sucrose.
24239581	8	11	theme	experiments	1067:1077	arg1	design					1057:1062	design	1057:1062	design of experiments	1057:1077	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	2	12	theme	mannitol	288:295	arg1	hemihydrate					297:307	mannitol hemihydrate	288:307	mannitol hemihydrate	288:307	The formation of mannitol hemihydrate is reported in several case studies; however, no systematic investigation has been performed so far.
24239581	3	13	theme	surface	472:478	arg1	modelling					480:488	response surface modelling	463:488	response surface modelling of mannitol hemihydrate formation	463:522	In this study, design of experiments was applied for response surface modelling of mannitol hemihydrate formation.
24239581	8	14	theme	mannitol	1205:1212	arg1	content					1214:1220	low relative mannitol content	1192:1220	low relative mannitol content	1192:1220	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	5	15	theme	mixed	790:794	arg1	design					796:801	a full factorial mixed design	773:801	a full factorial mixed design	773:801	Additionally, annealing as process parameter was included in a full factorial mixed design.
24239581	4	16	theme	solid	617:621	arg1	parameters					541:550	The formulation parameters	525:550	The formulation parameters investigated in a composite face-centred design	525:598	The formulation parameters investigated in a composite face-centred design were the overall solid content, protein concentration, protein type and the ratio between mannitol and sucrose.
24239581	4	16	theme	solid	617:621	arg1	content					623:629	the overall solid content	605:629	the overall solid content	605:629	The formulation parameters investigated in a composite face-centred design were the overall solid content, protein concentration, protein type and the ratio between mannitol and sucrose.
24239581	6	17	dep	R	858:858	arg1	0.93					873:876	0.93	873:876	0.93	873:876	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	6	17	dep	R	858:858	arg1	0.82					864:867	0.82	864:867	0.82	864:867	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	8	18	theme	prominent	1100:1108	arg1	factors					1110:1116	the most prominent factors	1091:1116	the most prominent factors enhancing the formation of hemihydrate	1091:1155	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	8	18	theme	prominent	1100:1108	arg1	concentration					1177:1189	a high protein concentration	1162:1189	a high protein concentration	1162:1189	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	5	19	theme	factorial	780:788	arg1	design					796:801	a full factorial mixed design	773:801	a full factorial mixed design	773:801	Additionally, annealing as process parameter was included in a full factorial mixed design.
24239581	2	20	theme	several	324:330	arg1	studies					337:343	several case studies	324:343	several case studies	324:343	The formation of mannitol hemihydrate is reported in several case studies; however, no systematic investigation has been performed so far.
24239581	8	21	theme	protein	1169:1175	arg1	factors					1110:1116	the most prominent factors	1091:1116	the most prominent factors enhancing the formation of hemihydrate	1091:1155	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	8	21	theme	protein	1169:1175	arg1	concentration					1177:1189	a high protein concentration	1162:1189	a high protein concentration	1162:1189	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	7	22	theme	process	960:966	arg1	annealing					978:986	the process parameter annealing	956:986	the process parameter annealing	956:986	Inclusion of the process parameter annealing resulted in models of similar quality.
24239581	8	23	from	-20°C	1239:1243	arg1	factors					1110:1116	the most prominent factors	1091:1116	the most prominent factors enhancing the formation of hemihydrate	1091:1155	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	8	23	from	-20°C	1239:1243	arg1	annealing					1226:1234	annealing	1226:1234	annealing	1226:1234	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	8	23	from	-20°C	1239:1243	arg1	content					1214:1220	low relative mannitol content	1192:1220	low relative mannitol content	1192:1220	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	8	23	from	-20°C	1239:1243	arg1	concentration					1177:1189	a high protein concentration	1162:1189	a high protein concentration	1162:1189	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	8	24	theme	high	1164:1167	arg1	factors					1110:1116	the most prominent factors	1091:1116	the most prominent factors enhancing the formation of hemihydrate	1091:1155	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	8	24	theme	high	1164:1167	arg1	concentration					1177:1189	a high protein concentration	1162:1189	a high protein concentration	1162:1189	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	6	25	with	models	822:827	arg1	goodness					841:848	a high goodness	834:848	a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89)	834:926	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	7	26	theme	annealing	978:986	arg1	Inclusion					943:951	Inclusion	943:951	Inclusion of the process parameter annealing	943:986	Inclusion of the process parameter annealing resulted in models of similar quality.
24239581	0	27	theme	mannitol	13:20	arg1	Formation					0:8	Formation	0:8	Formation of mannitol	0:20	Formation of mannitol hemihydrate in freeze-dried protein formulations--a design of experiment approach.
24239581	4	28	theme	face-centred	580:591	arg1	design					593:598	a composite face-centred design	568:598	a composite face-centred design	568:598	The formulation parameters investigated in a composite face-centred design were the overall solid content, protein concentration, protein type and the ratio between mannitol and sucrose.
24239581	6	29	dep	goodness	883:890	arg1	Q					907:907	Q(2)	907:910	Q(2): 0.78 and 0.89	907:925	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	1	30	theme	mannitol	163:170	arg1	form					155:158	this form	150:158	this form of mannitol	150:170	Since the discovery of mannitol hemihydrate, this form of mannitol has been seen as potentially negative with regard to the stability of pharmaceutical formulations.
24239581	1	30	theme	mannitol	163:170	arg1	negative					201:208	negative	201:208	negative	201:208	Since the discovery of mannitol hemihydrate, this form of mannitol has been seen as potentially negative with regard to the stability of pharmaceutical formulations.
24239581	8	31	theme	design	1057:1062	arg1	application					1042:1052	The successful application	1027:1052	The successful application of design of experiments	1027:1077	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	0	32	theme	freeze-dried	37:48	arg1	design					74:79	freeze-dried protein formulations--a design	37:79	freeze-dried protein formulations--a design of experiment approach	37:102	Formation of mannitol hemihydrate in freeze-dried protein formulations--a design of experiment approach.
24239581	4	33	theme	composite	570:578	arg1	design					593:598	a composite face-centred design	568:598	a composite face-centred design	568:598	The formulation parameters investigated in a composite face-centred design were the overall solid content, protein concentration, protein type and the ratio between mannitol and sucrose.
24239581	6	34	dep	fit	853:855	arg1	2					860:860	2	860:860	2	860:860	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	6	34	dep	fit	853:855	arg1	R					858:858	R	858:858	R(2)	858:861	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	8	35	theme	relative	1196:1203	arg1	content					1214:1220	low relative mannitol content	1192:1220	low relative mannitol content	1192:1220	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	3	36	theme	experiments	435:445	arg1	design					425:430	design	425:430	design of experiments	425:445	In this study, design of experiments was applied for response surface modelling of mannitol hemihydrate formation.
24239581	4	37	theme	formulation	529:539	arg1	parameters					541:550	The formulation parameters	525:550	The formulation parameters investigated in a composite face-centred design	525:598	The formulation parameters investigated in a composite face-centred design were the overall solid content, protein concentration, protein type and the ratio between mannitol and sucrose.
24239581	4	37	theme	formulation	529:539	arg1	content					623:629	the overall solid content	605:629	the overall solid content	605:629	The formulation parameters investigated in a composite face-centred design were the overall solid content, protein concentration, protein type and the ratio between mannitol and sucrose.
24239581	7	38	theme	parameter	968:976	arg1	annealing					978:986	the process parameter annealing	956:986	the process parameter annealing	956:986	Inclusion of the process parameter annealing resulted in models of similar quality.
24239581	6	39	theme	fit	853:855	arg1	goodness					841:848	a high goodness	834:848	a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89)	834:926	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	4	40	theme	protein	655:661	arg1	type					663:666	protein type	655:666	protein type	655:666	The formulation parameters investigated in a composite face-centred design were the overall solid content, protein concentration, protein type and the ratio between mannitol and sucrose.
24239581	0	41	theme	formulations--a	58:72	arg1	design					74:79	freeze-dried protein formulations--a design	37:79	freeze-dried protein formulations--a design of experiment approach	37:102	Formation of mannitol hemihydrate in freeze-dried protein formulations--a design of experiment approach.
24239581	6	42	theme	prediction	895:904	arg1	fit					853:855	fit	853:855	fit (R(2): 0.82 and 0.93)	853:877	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	6	42	theme	prediction	895:904	arg1	goodness					883:890	goodness	883:890	goodness of prediction (Q(2): 0.78 and 0.89)	883:926	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	0	43	theme	protein	50:56	arg1	design					74:79	freeze-dried protein formulations--a design	37:79	freeze-dried protein formulations--a design of experiment approach	37:102	Formation of mannitol hemihydrate in freeze-dried protein formulations--a design of experiment approach.
24239581	6	44	theme	goodness	883:890	arg1	goodness					841:848	a high goodness	834:848	a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89)	834:926	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	6	45	dep	Q	907:907	arg1	0.78					913:916	0.78	913:916	0.78	913:916	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	6	45	dep	Q	907:907	arg1	0.89					922:925	0.89	922:925	0.89	922:925	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	7	46	theme	similar	1010:1016	arg1	quality					1018:1024	similar quality	1010:1024	similar quality	1010:1024	Inclusion of the process parameter annealing resulted in models of similar quality.
24239581	1	47	theme	mannitol	128:135	arg1	hemihydrate					137:147	mannitol hemihydrate	128:147	mannitol hemihydrate	128:147	Since the discovery of mannitol hemihydrate, this form of mannitol has been seen as potentially negative with regard to the stability of pharmaceutical formulations.
24239581	8	48	theme	successful	1031:1040	arg1	application					1042:1052	The successful application	1027:1052	The successful application of design of experiments	1027:1077	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	6	49	theme	high	836:839	arg1	goodness					841:848	a high goodness	834:848	a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89)	834:926	For two proteins, models with a high goodness of fit (R(2): 0.82 and 0.93) and goodness of prediction (Q(2): 0.78 and 0.89) were achieved.
24239581	1	50	theme	hemihydrate	137:147	arg1	discovery					115:123	the discovery	111:123	the discovery of mannitol hemihydrate	111:147	Since the discovery of mannitol hemihydrate, this form of mannitol has been seen as potentially negative with regard to the stability of pharmaceutical formulations.
24239581	2	51	theme	case	332:335	arg1	studies					337:343	several case studies	324:343	several case studies	324:343	The formation of mannitol hemihydrate is reported in several case studies; however, no systematic investigation has been performed so far.
24239581	3	52	theme	response	463:470	arg1	modelling					480:488	response surface modelling	463:488	response surface modelling of mannitol hemihydrate formation	463:522	In this study, design of experiments was applied for response surface modelling of mannitol hemihydrate formation.
24239581	8	53	theme	low	1192:1194	arg1	content					1214:1220	low relative mannitol content	1192:1220	low relative mannitol content	1192:1220	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	3	54	theme	formation	514:522	arg1	modelling					480:488	response surface modelling	463:488	response surface modelling of mannitol hemihydrate formation	463:522	In this study, design of experiments was applied for response surface modelling of mannitol hemihydrate formation.
24239581	4	55	theme	protein	632:638	arg1	concentration					640:652	protein concentration	632:652	protein concentration	632:652	The formulation parameters investigated in a composite face-centred design were the overall solid content, protein concentration, protein type and the ratio between mannitol and sucrose.
24239581	8	56	theme	hemihydrate	1145:1155	arg1	formation					1132:1140	the formation	1128:1140	the formation of hemihydrate	1128:1155	The successful application of design of experiments showed that the most prominent factors enhancing the formation of hemihydrate were a high protein concentration, low relative mannitol content and annealing at -20°C.
24239581	2	57	theme	systematic	358:367	arg1	investigation					369:381	no systematic investigation	355:381	no systematic investigation	355:381	The formation of mannitol hemihydrate is reported in several case studies; however, no systematic investigation has been performed so far.
24961964	4	0	theme	functional	944:953	arg1	product					965:971	a new functional prebiotic product	938:971	a new functional prebiotic product	938:971	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	1	1	theme	kernels	228:234	arg1	extract					199:205	a hydrosoluble extract	184:205	a hydrosoluble extract of broken cashew nut kernels and passion fruit juice	184:258	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	1	2	theme	passion	240:246	arg1	juice					254:258	passion fruit juice	240:258	passion fruit juice	240:258	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	2	3	theme	sugar	572:576	arg1	concentrations					479:492	different concentrations	469:492	different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar	469:576	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	4	4	theme	cashew	1034:1039	arg1	kernels					1045:1051	cashew nut kernels	1034:1051	cashew nut kernels	1034:1051	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	2	5	used	used	400:403	arg2	design					389:394	A 2(2) central composite rotatable design	354:394	A 2(2) central composite rotatable design	354:394	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	2	6	theme	3	569:569	arg1	%					570:570	%	570:570	%	570:570	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	4	7	theme	kernels	1045:1051	arg1	properties					1020:1029	the nutritional and functional properties	989:1029	the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice	989:1120	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	1	8	theme	prebiotic	160:168	arg1	beverage					170:177	a prebiotic beverage	158:177	a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice	158:258	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	2	9	theme	nut	517:519	arg1	kernel					521:526	hydrosoluble cashew nut kernel	497:526	hydrosoluble cashew nut kernel	497:526	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	4	10	with	beverage	1127:1134	arg1	acceptance					1162:1171	satisfactory sensory acceptance	1141:1171	satisfactory sensory acceptance	1141:1171	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	2	11	theme	hydrosoluble	497:508	arg1	kernel					521:526	hydrosoluble cashew nut kernel	497:526	hydrosoluble cashew nut kernel	497:526	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	1	12	theme	hydrosoluble	186:197	arg1	extract					199:205	a hydrosoluble extract	184:205	a hydrosoluble extract of broken cashew nut kernels and passion fruit juice	184:258	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	2	13	theme	fruit	537:541	arg1	juice					543:547	passion fruit juice	529:547	passion fruit juice	529:547	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	3	14	theme	satisfactory	695:706	arg1	acceptance					708:717	satisfactory acceptance	695:717	satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice	695:860	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	1	15	theme	UNLABELLED	107:116	arg1	aim					122:124	UNLABELLED The aim	107:124	UNLABELLED The aim of this research	107:141	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	3	16	theme	nut	795:797	arg1	kernels					799:805	hydrosoluble cashew nut kernels	775:805	hydrosoluble cashew nut kernels	775:805	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	4	17	theme	passion	1102:1108	arg1	juice					1116:1120	passion fruit juice	1102:1120	passion fruit juice	1102:1120	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	3	18	theme	hydrosoluble	775:786	arg1	kernels					799:805	hydrosoluble cashew nut kernels	775:805	hydrosoluble cashew nut kernels	775:805	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	4	19	theme	oligofructose	1057:1069	arg1	properties					1020:1029	the nutritional and functional properties	989:1029	the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice	989:1120	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	5	20	theme	new	1204:1206	arg1	alternative					1208:1218	a new alternative	1202:1218	a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits	1202:1357	This new product emerges as a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits.
24961964	4	21	theme	study	905:909	arg1	result					890:895	a result	888:895	a result of this study	888:909	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	3	22	theme	use	768:770	arg1	criteria					733:740	the criteria	729:740	the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels	729:805	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	0	23	theme	nut	70:72	arg1	kernels					74:80	cashew nut kernels	63:80	cashew nut kernels	63:80	Optimization of the acceptance of prebiotic beverage made from cashew nut kernels and passion fruit juice.
24961964	3	24	theme	action	757:762	arg1	criteria					733:740	the criteria	729:740	the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels	729:805	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	4	25	theme	sensory	1154:1160	arg1	acceptance					1162:1171	satisfactory sensory acceptance	1141:1171	satisfactory sensory acceptance	1141:1171	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	0	26	theme	fruit	94:98	arg1	juice					100:104	passion fruit juice	86:104	passion fruit juice	86:104	Optimization of the acceptance of prebiotic beverage made from cashew nut kernels and passion fruit juice.
24961964	1	27	theme	attributes	342:351	arg1	acceptance					316:325	acceptance	316:325	acceptance of its sensory attributes	316:351	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	4	28	theme	juice	1116:1120	arg1	properties					1088:1097	the sensory properties	1076:1097	the sensory properties of passion fruit juice	1076:1120	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	5	29	theme	cashew	1260:1265	arg1	kernels					1271:1277	broken cashew nut kernels	1253:1277	broken cashew nut kernels	1253:1277	This new product emerges as a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits.
24961964	1	30	theme	fruit	248:252	arg1	juice					254:258	passion fruit juice	240:258	passion fruit juice	240:258	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	3	31	with	formulation	678:688	arg1	acceptance					708:717	satisfactory acceptance	695:717	satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice	695:860	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	4	32	theme	new	940:942	arg1	product					965:971	a new functional prebiotic product	938:971	a new functional prebiotic product	938:971	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	2	33	theme	rotatable	379:387	arg1	design					389:394	A 2(2) central composite rotatable design	354:394	A 2(2) central composite rotatable design	354:394	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	4	34	with	kernels	1045:1051	arg1	properties					1088:1097	the sensory properties	1076:1097	the sensory properties of passion fruit juice	1076:1120	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	3	35	theme	passion	842:848	arg1	juice					856:860	passion fruit juice	842:860	33% passion fruit juice	838:860	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	5	36	theme	industrial	1228:1237	arg1	processing					1239:1248	the industrial processing	1224:1248	the industrial processing	1224:1248	This new product emerges as a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits.
24961964	2	37	theme	central	361:367	arg1	design					389:394	A 2(2) central composite rotatable design	354:394	A 2(2) central composite rotatable design	354:394	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	1	38	theme	surface	275:281	arg1	methodology					283:293	response surface methodology	266:293	response surface methodology in order to optimize acceptance of its sensory attributes	266:351	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	0	39	theme	beverage	44:51	arg1	acceptance					20:29	the acceptance	16:29	the acceptance of prebiotic beverage	16:51	Optimization of the acceptance of prebiotic beverage made from cashew nut kernels and passion fruit juice.
24961964	5	40	theme	market	1300:1305	arg1	value					1307:1311	very low market value	1291:1311	very low market value	1291:1311	This new product emerges as a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits.
24961964	4	41	theme	nutritional	993:1003	arg1	properties					1020:1029	the nutritional and functional properties	989:1029	the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice	989:1120	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	3	42	theme	surface	599:605	arg1	methodology					607:617	response surface methodology	590:617	response surface methodology	590:617	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	5	43	theme	kernels	1271:1277	arg1	processing					1239:1248	the industrial processing	1224:1248	the industrial processing	1224:1248	This new product emerges as a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits.
24961964	1	44	theme	broken	210:215	arg1	kernels					228:234	broken cashew nut kernels	210:234	broken cashew nut kernels	210:234	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	4	45	theme	prebiotic	955:963	arg1	product					965:971	a new functional prebiotic product	938:971	a new functional prebiotic product	938:971	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	1	46	theme	nut	224:226	arg1	kernels					228:234	broken cashew nut kernels	210:234	broken cashew nut kernels	210:234	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	2	47	theme	oligofructose	550:562	arg1	concentrations					479:492	different concentrations	469:492	different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar	469:576	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	2	48	theme	juice	543:547	arg1	concentrations					479:492	different concentrations	469:492	different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar	469:576	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	4	49	theme	nut	1041:1043	arg1	kernels					1045:1051	cashew nut kernels	1034:1051	cashew nut kernels	1034:1051	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	4	50	with	oligofructose	1057:1069	arg1	properties					1088:1097	the sensory properties	1076:1097	the sensory properties of passion fruit juice	1076:1120	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	2	51	theme	%	570:570	arg1	sugar					572:576	3% sugar	569:576	3% sugar	569:576	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	4	52	theme	functional	1009:1018	arg1	properties					1020:1029	the nutritional and functional properties	989:1029	the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice	989:1120	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	2	53	theme	cashew	510:515	arg1	kernel					521:526	hydrosoluble cashew nut kernel	497:526	hydrosoluble cashew nut kernel	497:526	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	4	54	dep	APPLICATION	873:883	arg1	result					890:895	a result	888:895	a result of this study	888:909	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	2	55	theme	passion	529:535	arg1	juice					543:547	passion fruit juice	529:547	passion fruit juice	529:547	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	4	56	theme	fruit	1110:1114	arg1	juice					1116:1120	passion fruit juice	1102:1120	passion fruit juice	1102:1120	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	1	57	from	extract	199:205	arg1	beverage					170:177	a prebiotic beverage	158:177	a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice	158:258	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	3	58	dep	%	840:840	arg1	juice					856:860	passion fruit juice	842:860	33% passion fruit juice	838:860	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	3	59	theme	kernels	799:805	arg1	use					768:770	use	768:770	use of hydrosoluble cashew nut kernels	768:805	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	3	59	theme	kernels	799:805	arg1	action					757:762	bifidogenic action	745:762	bifidogenic action	745:762	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	2	60	theme	kernel	521:526	arg1	concentrations					479:492	different concentrations	469:492	different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar	469:576	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	3	61	theme	bifidogenic	745:755	arg1	action					757:762	bifidogenic action	745:762	bifidogenic action	745:762	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	0	62	theme	cashew	63:68	arg1	kernels					74:80	cashew nut kernels	63:80	cashew nut kernels	63:80	Optimization of the acceptance of prebiotic beverage made from cashew nut kernels and passion fruit juice.
24961964	3	63	theme	cashew	788:793	arg1	kernels					799:805	hydrosoluble cashew nut kernels	775:805	hydrosoluble cashew nut kernels	775:805	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	4	64	theme	sensory	1080:1086	arg1	properties					1088:1097	the sensory properties	1076:1097	the sensory properties of passion fruit juice	1076:1120	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	4	65	theme	PRACTICAL	863:871	arg1	APPLICATION					873:883	PRACTICAL APPLICATION	863:883	PRACTICAL APPLICATION	863:883	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	1	66	theme	research	134:141	arg1	aim					122:124	UNLABELLED The aim	107:124	UNLABELLED The aim of this research	107:141	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	4	67	theme	satisfactory	1141:1152	arg1	acceptance					1162:1171	satisfactory sensory acceptance	1141:1171	satisfactory sensory acceptance	1141:1171	PRACTICAL APPLICATION As a result of this study, it was possible to obtain a new functional prebiotic product, which combined the nutritional and functional properties of cashew nut kernels and oligofructose with the sensory properties of passion fruit juice in a beverage with satisfactory sensory acceptance.
24961964	5	68	theme	new	1179:1181	arg1	product					1183:1189	This new product	1174:1189	This new product	1174:1189	This new product emerges as a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits.
24961964	0	69	theme	passion	86:92	arg1	juice					100:104	passion fruit juice	86:104	passion fruit juice	86:104	Optimization of the acceptance of prebiotic beverage made from cashew nut kernels and passion fruit juice.
24961964	2	70	theme	different	469:477	arg1	concentrations					479:492	different concentrations	469:492	different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar	469:576	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	1	71	theme	sensory	334:340	arg1	attributes					342:351	its sensory attributes	330:351	its sensory attributes	330:351	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	5	72	theme	broken	1253:1258	arg1	kernels					1271:1277	broken cashew nut kernels	1253:1277	broken cashew nut kernels	1253:1277	This new product emerges as a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits.
24961964	0	73	theme	acceptance	20:29	arg1	Optimization					0:11	Optimization	0:11	Optimization of the acceptance of prebiotic beverage	0:51	Optimization of the acceptance of prebiotic beverage made from cashew nut kernels and passion fruit juice.
24961964	1	74	theme	juice	254:258	arg1	extract					199:205	a hydrosoluble extract	184:205	a hydrosoluble extract of broken cashew nut kernels and passion fruit juice	184:258	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	3	75	dep	%	818:818	arg1	oligofructose					820:832	oligofructose	820:832	14% oligofructose	816:832	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	2	76	theme	composite	369:377	arg1	design					389:394	A 2(2) central composite rotatable design	354:394	A 2(2) central composite rotatable design	354:394	A 2(2) central composite rotatable design was used, which produced 9 formulations, which were then evaluated using different concentrations of hydrosoluble cashew nut kernel, passion fruit juice, oligofructose, and 3% sugar.
24961964	0	77	theme	prebiotic	34:42	arg1	beverage					44:51	prebiotic beverage	34:51	prebiotic beverage	34:51	Optimization of the acceptance of prebiotic beverage made from cashew nut kernels and passion fruit juice.
24961964	1	78	theme	response	266:273	arg1	methodology					283:293	response surface methodology	266:293	response surface methodology in order to optimize acceptance of its sensory attributes	266:351	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
24961964	3	79	theme	fruit	850:854	arg1	juice					856:860	passion fruit juice	842:860	33% passion fruit juice	838:860	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	3	80	theme	methodology	607:617	arg1	use					583:585	The use	579:585	The use of response surface methodology to interpret the sensory data	579:647	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	5	81	theme	low	1296:1298	arg1	value					1307:1311	very low market value	1291:1311	very low market value	1291:1311	This new product emerges as a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits.
24961964	3	82	theme	response	590:597	arg1	methodology					607:617	response surface methodology	590:617	response surface methodology	590:617	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	5	83	theme	nut	1267:1269	arg1	kernels					1271:1277	broken cashew nut kernels	1253:1277	broken cashew nut kernels	1253:1277	This new product emerges as a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits.
24961964	5	84	contain	have	1286:1289	arg2	value					1307:1311	very low market value	1291:1311	very low market value	1291:1311	This new product emerges as a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits.
24961964	5	84	contain	have	1286:1289	arg1	processing					1239:1248	the industrial processing	1224:1248	the industrial processing	1224:1248	This new product emerges as a new alternative for the industrial processing of broken cashew nut kernels, which have very low market value, enabling this sector to increase its profits.
24961964	3	85	theme	sensory	636:642	arg1	data					644:647	the sensory data	632:647	the sensory data	632:647	The use of response surface methodology to interpret the sensory data made it possible to obtain a formulation with satisfactory acceptance which met the criteria of bifidogenic action and use of hydrosoluble cashew nut kernels by using 14% oligofructose and 33% passion fruit juice.
24961964	1	86	theme	cashew	217:222	arg1	kernels					228:234	broken cashew nut kernels	210:234	broken cashew nut kernels	210:234	UNLABELLED The aim of this research was to develop a prebiotic beverage from a hydrosoluble extract of broken cashew nut kernels and passion fruit juice using response surface methodology in order to optimize acceptance of its sensory attributes.
28507244	7	0	theme	primary	1722:1728	arg1	site					1750:1753	its primary d-Ala-d-Ala binding site	1718:1753	its primary d-Ala-d-Ala binding site through Edman degradation	1718:1779	Even after the loss of its primary d-Ala-d-Ala binding site through Edman degradation, desleucyl-oritavancin exhibits potent antimicrobial activities through its still-functioning secondary binding site.
28507244	2	1	theme	binding	411:417	arg1	site					419:422	a secondary binding site	399:422	a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall	399:503	Oritavancin differs from vancomycin by a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall.
28507244	6	2	theme	peptidoglycan	1364:1376	arg1	composition					1378:1388	peptidoglycan composition	1364:1388	peptidoglycan composition	1364:1388	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	9	3	theme	extracted	2225:2233	arg1	chromatograms					2239:2251	extracted ion chromatograms	2225:2251	extracted ion chromatograms	2225:2251	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus was performed by determining the relative abundances of 83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms.
28507244	5	4	with	N-deacetylation	1322:1336	arg1	structure					1262:1270	a tetrapeptide-stem structure	1242:1270	a tetrapeptide-stem structure	1242:1270	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	3	5	theme	peptidoglycan	600:612	arg1	composition					614:624	the peptidoglycan composition	596:624	the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS)	596:781	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	8	6	theme	binding	1988:1994	arg1	site					1996:1999	desleucyl-oritavancin's secondary binding site	1954:1999	desleucyl-oritavancin's secondary binding site	1954:1999	In this study, we characterized the mode of action for desleucyl-oritavancin's secondary binding site using LC-MS.
28507244	4	7	theme	binding	976:982	arg1	site					984:987	its functional secondary binding site	951:987	its functional secondary binding site	951:987	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	5	8	with	reduction	1172:1180	arg1	structure					1262:1270	a tetrapeptide-stem structure	1242:1270	a tetrapeptide-stem structure	1242:1270	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	6	9	theme	cell	1470:1473	arg1	disorder					1480:1487	cell wall disorder	1470:1487	cell wall disorder	1470:1487	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	2	10	theme	side	341:344	arg1	chain					346:350	a hydrophobic side chain	327:350	a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall	327:503	Oritavancin differs from vancomycin by a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall.
28507244	6	11	with	antibiotic	1575:1584	arg1	site					1611:1614	a secondary binding site	1591:1614	a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall	1591:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	5	12	theme	quantitative	999:1010	arg1	analysis					1038:1045	Accurate quantitative peptidoglycan composition analysis	990:1045	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin	990:1161	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	10	13	theme	composition	2312:2322	arg1	analysis					2324:2331	quantitative peptidoglycan composition analysis	2285:2331	quantitative peptidoglycan composition analysis by LC-MS	2285:2340	Our work highlights the use of quantitative peptidoglycan composition analysis by LC-MS to provide insights into the mode of action of glycopeptide antibiotics.
28507244	3	14	theme	aureus	644:649	arg1	composition					614:624	the peptidoglycan composition	596:624	the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS)	596:781	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	5	15	theme	composition	1026:1036	arg1	analysis					1038:1045	Accurate quantitative peptidoglycan composition analysis	990:1045	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin	990:1161	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	6	16	dep	Oritavancin	1541:1551	arg1	lipoglycopeptide					1558:1573	a lipoglycopeptide	1556:1573	a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall	1556:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	10	17	theme	quantitative	2285:2296	arg1	analysis					2324:2331	quantitative peptidoglycan composition analysis	2285:2331	quantitative peptidoglycan composition analysis by LC-MS	2285:2340	Our work highlights the use of quantitative peptidoglycan composition analysis by LC-MS to provide insights into the mode of action of glycopeptide antibiotics.
28507244	0	18	theme	Mass	138:141	arg1	Spectrometry					143:154	Mass Spectrometry	138:154	Mass Spectrometry	138:154	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.
28507244	1	19	theme	vancomycin-resistant	242:261	arg1	pathogens					277:285	vancomycin-resistant Gram-positive pathogens	242:285	vancomycin-resistant Gram-positive pathogens	242:285	Oritavancin is a lipoglycopeptide antibiotic that exhibits potent activities against vancomycin-resistant Gram-positive pathogens.
28507244	5	20	theme	tetrapeptide-stem	1244:1260	arg1	structure					1262:1270	a tetrapeptide-stem structure	1242:1270	a tetrapeptide-stem structure	1242:1270	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	4	21	theme	degradation	818:828	arg1	product					830:836	an Edman degradation product	809:836	an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site	809:987	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	4	21	theme	degradation	818:828	arg1	Desleucyl-oritavancin					784:804	Desleucyl-oritavancin	784:804	Desleucyl-oritavancin	784:804	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	6	22	theme	maturation.IMPORTANCE	1519:1539	arg1	Oritavancin					1541:1551	cell wall maturation.IMPORTANCE Oritavancin	1509:1551	cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall	1509:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	5	23	theme	cross-linking	1199:1211	arg1	O-acetylation					1283:1295	decreased O-acetylation	1273:1295	decreased O-acetylation of MurNAc	1273:1305	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	5	23	theme	cross-linking	1199:1211	arg1	reduction					1172:1180	a reduction	1170:1180	a reduction of peptidoglycan cross-linking	1170:1211	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	5	23	theme	cross-linking	1199:1211	arg1	N-deacetylation					1322:1336	increased N-deacetylation	1312:1336	increased N-deacetylation of GlcNAc	1312:1346	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	5	23	theme	cross-linking	1199:1211	arg1	muropeptides					1224:1235	increased muropeptides	1214:1235	increased muropeptides with a tetrapeptide-stem structure	1214:1270	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	9	24	theme	Peptidoglycan	2014:2026	arg1	analysis					2040:2047	Peptidoglycan composition analysis	2014:2047	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus	2014:2090	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus was performed by determining the relative abundances of 83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms.
28507244	6	25	theme	cell	1509:1512	arg1	Oritavancin					1541:1551	cell wall maturation.IMPORTANCE Oritavancin	1509:1551	cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall	1509:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	6	26	from	structure	1667:1675	arg1	wall					1689:1692	the cell wall	1680:1692	the cell wall	1680:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	3	27	theme	subinhibitory	701:713	arg1	concentration					715:727	subinhibitory concentration	701:727	subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS)	701:781	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	7	28	theme	secondary	1875:1883	arg1	site					1893:1896	its still-functioning secondary binding site	1853:1896	its still-functioning secondary binding site	1853:1896	Even after the loss of its primary d-Ala-d-Ala binding site through Edman degradation, desleucyl-oritavancin exhibits potent antimicrobial activities through its still-functioning secondary binding site.
28507244	6	29	link	cross-linked	1633:1644	arg1	structure					1667:1675	the cross-linked peptidoglycan bridge structure	1629:1675	the cross-linked peptidoglycan bridge structure in the cell wall	1629:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	5	30	theme	MurNAc	1300:1305	arg1	O-acetylation					1283:1295	decreased O-acetylation	1273:1295	decreased O-acetylation of MurNAc	1273:1305	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	5	30	theme	MurNAc	1300:1305	arg1	reduction					1172:1180	a reduction	1170:1180	a reduction of peptidoglycan cross-linking	1170:1211	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	5	30	theme	MurNAc	1300:1305	arg1	N-deacetylation					1322:1336	increased N-deacetylation	1312:1336	increased N-deacetylation of GlcNAc	1312:1346	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	5	30	theme	MurNAc	1300:1305	arg1	muropeptides					1224:1235	increased muropeptides	1214:1235	increased muropeptides with a tetrapeptide-stem structure	1214:1270	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	3	31	theme	desleucyl-oritavancin	676:696	arg1	presence					664:671	the presence	660:671	the presence of desleucyl-oritavancin	660:696	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	1	32	gly	lipoglycopeptide	174:189	arg2	lipoglycopeptide					174:189	lipoglycopeptide	174:189	lipoglycopeptide	174:189	Oritavancin is a lipoglycopeptide antibiotic that exhibits potent activities against vancomycin-resistant Gram-positive pathogens.
28507244	5	33	theme	increased	1214:1222	arg1	muropeptides					1224:1235	increased muropeptides	1214:1235	increased muropeptides with a tetrapeptide-stem structure	1214:1270	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	9	34	theme	aureus	2085:2090	arg1	analysis					2040:2047	Peptidoglycan composition analysis	2014:2047	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus	2014:2090	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus was performed by determining the relative abundances of 83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms.
28507244	0	35	theme	Peptidoglycan	100:112	arg1	Analysis					126:133	a Quantitative Peptidoglycan Composition Analysis	85:133	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.	0:155	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.
28507244	7	36	theme	potent	1813:1818	arg1	activities					1834:1843	potent antimicrobial activities	1813:1843	potent antimicrobial activities	1813:1843	Even after the loss of its primary d-Ala-d-Ala binding site through Edman degradation, desleucyl-oritavancin exhibits potent antimicrobial activities through its still-functioning secondary binding site.
28507244	5	37	with	muropeptides	1224:1235	arg1	structure					1262:1270	a tetrapeptide-stem structure	1242:1270	a tetrapeptide-stem structure	1242:1270	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	10	38	gly	glycopeptide	2389:2400	arg2	glycopeptide					2389:2400	glycopeptide antibiotics	2389:2412	glycopeptide antibiotics	2389:2412	Our work highlights the use of quantitative peptidoglycan composition analysis by LC-MS to provide insights into the mode of action of glycopeptide antibiotics.
28507244	6	39	theme	cell	1684:1687	arg1	wall					1689:1692	the cell wall	1680:1692	the cell wall	1680:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	9	40	theme	relative	2125:2132	arg1	abundances					2134:2143	the relative abundances	2121:2143	the relative abundances of 83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms	2121:2251	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus was performed by determining the relative abundances of 83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms.
28507244	4	41	theme	potent	867:872	arg1	activities					888:897	potent antibacterial activities	867:897	potent antibacterial activities	867:897	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	3	42	theme	action	518:523	arg1	mode					510:513	The mode	506:513	The mode of action of secondary binding site	506:549	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	0	43	theme	Staphylococcus	14:27	arg1	Biosynthesis					46:57	Staphylococcus aureus Cell Wall Biosynthesis	14:57	Staphylococcus aureus Cell Wall Biosynthesis	14:57	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.
28507244	8	44	theme	secondary	1978:1986	arg1	site					1996:1999	desleucyl-oritavancin's secondary binding site	1954:1999	desleucyl-oritavancin's secondary binding site	1954:1999	In this study, we characterized the mode of action for desleucyl-oritavancin's secondary binding site using LC-MS.
28507244	3	45	theme	chromatography-mass	742:760	arg1	LC-MS					776:780	LC-MS	776:780	LC-MS	776:780	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	3	45	theme	chromatography-mass	742:760	arg1	spectrometry					762:773	liquid chromatography-mass spectrometry	735:773	liquid chromatography-mass spectrometry (LC-MS)	735:781	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	0	46	theme	Cell	36:39	arg1	Biosynthesis					46:57	Staphylococcus aureus Cell Wall Biosynthesis	14:57	Staphylococcus aureus Cell Wall Biosynthesis	14:57	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.
28507244	6	47	theme	bridge	1660:1665	arg1	structure					1667:1675	the cross-linked peptidoglycan bridge structure	1629:1675	the cross-linked peptidoglycan bridge structure in the cell wall	1629:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	7	48	theme	Edman	1763:1767	arg1	degradation					1769:1779	Edman degradation	1763:1779	Edman degradation	1763:1779	Even after the loss of its primary d-Ala-d-Ala binding site through Edman degradation, desleucyl-oritavancin exhibits potent antimicrobial activities through its still-functioning secondary binding site.
28507244	9	49	theme	muropeptide	2151:2161	arg1	ions					2163:2166	83 muropeptide ions	2148:2166	83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms	2148:2251	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus was performed by determining the relative abundances of 83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms.
28507244	4	50	theme	functional	955:964	arg1	site					984:987	its functional secondary binding site	951:987	its functional secondary binding site	951:987	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	3	51	theme	site	546:549	arg1	action					518:523	action	518:523	action of secondary binding site	518:549	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	0	52	theme	Biosynthesis	46:57	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.	0:155	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.
28507244	5	53	theme	GlcNAc	1341:1346	arg1	O-acetylation					1283:1295	decreased O-acetylation	1273:1295	decreased O-acetylation of MurNAc	1273:1305	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	5	53	theme	GlcNAc	1341:1346	arg1	reduction					1172:1180	a reduction	1170:1180	a reduction of peptidoglycan cross-linking	1170:1211	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	5	53	theme	GlcNAc	1341:1346	arg1	N-deacetylation					1322:1336	increased N-deacetylation	1312:1336	increased N-deacetylation of GlcNAc	1312:1346	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	5	53	theme	GlcNAc	1341:1346	arg1	muropeptides					1224:1235	increased muropeptides	1214:1235	increased muropeptides with a tetrapeptide-stem structure	1214:1270	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	7	54	theme	site	1750:1753	arg1	loss					1710:1713	the loss	1706:1713	the loss of its primary d-Ala-d-Ala binding site through Edman degradation	1706:1779	Even after the loss of its primary d-Ala-d-Ala binding site through Edman degradation, desleucyl-oritavancin exhibits potent antimicrobial activities through its still-functioning secondary binding site.
28507244	5	55	theme	peptidoglycan	1185:1197	arg1	cross-linking					1199:1211	peptidoglycan cross-linking	1185:1211	peptidoglycan cross-linking	1185:1211	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	2	56	link	cross-linked	461:472	arg1	peptidoglycan					474:486	the cross-linked peptidoglycan	457:486	the cross-linked peptidoglycan in the cell wall	457:503	Oritavancin differs from vancomycin by a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall.
28507244	4	57	theme	d-Ala-d-Ala	919:929	arg1	site					939:942	the damaged d-Ala-d-Ala binding site	907:942	the damaged d-Ala-d-Ala binding site due to its functional secondary binding site	907:987	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	3	58	theme	secondary	528:536	arg1	site					546:549	secondary binding site	528:549	secondary binding site	528:549	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	7	59	theme	d-Ala-d-Ala	1730:1740	arg1	site					1750:1753	its primary d-Ala-d-Ala binding site	1718:1753	its primary d-Ala-d-Ala binding site through Edman degradation	1718:1779	Even after the loss of its primary d-Ala-d-Ala binding site through Edman degradation, desleucyl-oritavancin exhibits potent antimicrobial activities through its still-functioning secondary binding site.
28507244	2	60	from	peptidoglycan	474:486	arg1	wall					500:503	the cell wall	491:503	the cell wall	491:503	Oritavancin differs from vancomycin by a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall.
28507244	10	61	theme	antibiotics	2402:2412	arg1	action					2379:2384	action	2379:2384	action of glycopeptide antibiotics	2379:2412	Our work highlights the use of quantitative peptidoglycan composition analysis by LC-MS to provide insights into the mode of action of glycopeptide antibiotics.
28507244	0	62	dep	Inhibition	0:9	arg1	Analysis					126:133	a Quantitative Peptidoglycan Composition Analysis	85:133	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.	0:155	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.
28507244	8	63	theme	action	1943:1948	arg1	mode					1935:1938	the mode	1931:1938	the mode of action for desleucyl-oritavancin's secondary binding site	1931:1999	In this study, we characterized the mode of action for desleucyl-oritavancin's secondary binding site using LC-MS.
28507244	9	64	theme	ion	2235:2237	arg1	chromatograms					2239:2251	extracted ion chromatograms	2225:2251	extracted ion chromatograms	2225:2251	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus was performed by determining the relative abundances of 83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms.
28507244	2	65	theme	secondary	401:409	arg1	site					419:422	a secondary binding site	399:422	a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall	399:503	Oritavancin differs from vancomycin by a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall.
28507244	6	66	theme	wall	1475:1478	arg1	disorder					1480:1487	cell wall disorder	1470:1487	cell wall disorder	1470:1487	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	1	67	theme	potent	216:221	arg1	activities					223:232	potent activities	216:232	potent activities against vancomycin-resistant Gram-positive pathogens	216:285	Oritavancin is a lipoglycopeptide antibiotic that exhibits potent activities against vancomycin-resistant Gram-positive pathogens.
28507244	5	68	theme	Accurate	990:997	arg1	analysis					1038:1045	Accurate quantitative peptidoglycan composition analysis	990:1045	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin	990:1161	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	6	69	theme	cross-linked	1633:1644	arg1	structure					1667:1675	the cross-linked peptidoglycan bridge structure	1629:1675	the cross-linked peptidoglycan bridge structure in the cell wall	1629:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	4	70	theme	secondary	966:974	arg1	site					984:987	its functional secondary binding site	951:987	its functional secondary binding site	951:987	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	5	71	theme	peptidoglycan	1012:1024	arg1	analysis					1038:1045	Accurate quantitative peptidoglycan composition analysis	990:1045	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin	990:1161	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	10	72	theme	peptidoglycan	2298:2310	arg1	analysis					2324:2331	quantitative peptidoglycan composition analysis	2285:2331	quantitative peptidoglycan composition analysis by LC-MS	2285:2340	Our work highlights the use of quantitative peptidoglycan composition analysis by LC-MS to provide insights into the mode of action of glycopeptide antibiotics.
28507244	6	73	theme	antibiotic	1575:1584	arg1	lipoglycopeptide					1558:1573	a lipoglycopeptide	1556:1573	a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall	1556:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	2	74	theme	drug	368:371	arg1	disaccharide					373:384	the drug disaccharide	364:384	the drug disaccharide	364:384	Oritavancin differs from vancomycin by a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall.
28507244	6	75	gly	lipoglycopeptide	1558:1573	arg2	lipoglycopeptide					1558:1573	a lipoglycopeptide	1556:1573	a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall	1556:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	1	76	theme	Gram-positive	263:275	arg1	pathogens					277:285	vancomycin-resistant Gram-positive pathogens	242:285	vancomycin-resistant Gram-positive pathogens	242:285	Oritavancin is a lipoglycopeptide antibiotic that exhibits potent activities against vancomycin-resistant Gram-positive pathogens.
28507244	2	77	attach	attached	352:359	arg1	disaccharide					373:384	the drug disaccharide	364:384	the drug disaccharide	364:384	Oritavancin differs from vancomycin by a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall.
28507244	2	77	attach	attached	352:359	arg2	chain					346:350	a hydrophobic side chain	327:350	a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall	327:503	Oritavancin differs from vancomycin by a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall.
28507244	6	78	theme	peptidoglycan	1437:1449	arg1	template					1451:1458	the peptidoglycan template	1433:1458	the peptidoglycan template	1433:1458	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	6	79	theme	binding	1603:1609	arg1	site					1611:1614	a secondary binding site	1591:1614	a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall	1591:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	5	80	theme	muropeptide	1059:1069	arg1	ions					1071:1074	83 muropeptide ions	1056:1074	83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin	1056:1161	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	7	81	theme	binding	1885:1891	arg1	site					1893:1896	its still-functioning secondary binding site	1853:1896	its still-functioning secondary binding site	1853:1896	Even after the loss of its primary d-Ala-d-Ala binding site through Edman degradation, desleucyl-oritavancin exhibits potent antimicrobial activities through its still-functioning secondary binding site.
28507244	4	82	theme	Edman	812:816	arg1	product					830:836	an Edman degradation product	809:836	an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site	809:987	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	4	82	theme	Edman	812:816	arg1	Desleucyl-oritavancin					784:804	Desleucyl-oritavancin	784:804	Desleucyl-oritavancin	784:804	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	5	83	with	O-acetylation	1283:1295	arg1	structure					1262:1270	a tetrapeptide-stem structure	1242:1270	a tetrapeptide-stem structure	1242:1270	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	10	84	theme	analysis	2324:2331	arg1	use					2278:2280	the use	2274:2280	the use of quantitative peptidoglycan composition analysis by LC-MS to provide insights into the mode of action of glycopeptide antibiotics	2274:2412	Our work highlights the use of quantitative peptidoglycan composition analysis by LC-MS to provide insights into the mode of action of glycopeptide antibiotics.
28507244	6	85	theme	wall	1514:1517	arg1	Oritavancin					1541:1551	cell wall maturation.IMPORTANCE Oritavancin	1509:1551	cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall	1509:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	2	86	theme	hydrophobic	329:339	arg1	chain					346:350	a hydrophobic side chain	327:350	a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall	327:503	Oritavancin differs from vancomycin by a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall.
28507244	7	87	theme	still-functioning	1857:1873	arg1	site					1893:1896	its still-functioning secondary binding site	1853:1896	its still-functioning secondary binding site	1853:1896	Even after the loss of its primary d-Ala-d-Ala binding site through Edman degradation, desleucyl-oritavancin exhibits potent antimicrobial activities through its still-functioning secondary binding site.
28507244	9	88	theme	composition	2028:2038	arg1	analysis					2040:2047	Peptidoglycan composition analysis	2014:2047	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus	2014:2090	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus was performed by determining the relative abundances of 83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms.
28507244	3	89	from	changes	585:591	arg1	composition					614:624	the peptidoglycan composition	596:624	the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS)	596:781	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	6	90	theme	secondary	1593:1601	arg1	site					1611:1614	a secondary binding site	1591:1614	a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall	1591:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	0	91	theme	Quantitative	87:98	arg1	Analysis					126:133	a Quantitative Peptidoglycan Composition Analysis	85:133	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.	0:155	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.
28507244	7	92	theme	antimicrobial	1820:1832	arg1	activities					1834:1843	potent antimicrobial activities	1813:1843	potent antimicrobial activities	1813:1843	Even after the loss of its primary d-Ala-d-Ala binding site through Edman degradation, desleucyl-oritavancin exhibits potent antimicrobial activities through its still-functioning secondary binding site.
28507244	10	93	theme	action	2379:2384	arg1	mode					2371:2374	the mode	2367:2374	the mode of action of glycopeptide antibiotics	2367:2412	Our work highlights the use of quantitative peptidoglycan composition analysis by LC-MS to provide insights into the mode of action of glycopeptide antibiotics.
28507244	0	94	theme	Composition	114:124	arg1	Analysis					126:133	a Quantitative Peptidoglycan Composition Analysis	85:133	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.	0:155	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.
28507244	5	95	theme	increased	1312:1320	arg1	N-deacetylation					1322:1336	increased N-deacetylation	1312:1336	increased N-deacetylation of GlcNAc	1312:1346	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	5	96	theme	aureus	1109:1114	arg1	walls					1097:1101	cell walls	1092:1101	cell walls of S. aureus grown in the presence of desleucyl-oritavancin	1092:1161	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	4	97	theme	antibacterial	874:886	arg1	activities					888:897	potent antibacterial activities	867:897	potent antibacterial activities	867:897	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	6	98	from	changes	1353:1359	arg1	composition					1378:1388	peptidoglycan composition	1364:1388	peptidoglycan composition	1364:1388	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	5	99	theme	decreased	1273:1281	arg1	O-acetylation					1283:1295	decreased O-acetylation	1273:1295	decreased O-acetylation of MurNAc	1273:1305	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	5	100	theme	cell	1092:1095	arg1	walls					1097:1101	cell walls	1092:1101	cell walls of S. aureus grown in the presence of desleucyl-oritavancin	1092:1161	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	4	101	theme	oritavancin	841:851	arg1	product					830:836	an Edman degradation product	809:836	an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site	809:987	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	4	101	theme	oritavancin	841:851	arg1	Desleucyl-oritavancin					784:804	Desleucyl-oritavancin	784:804	Desleucyl-oritavancin	784:804	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	0	102	theme	aureus	29:34	arg1	Biosynthesis					46:57	Staphylococcus aureus Cell Wall Biosynthesis	14:57	Staphylococcus aureus Cell Wall Biosynthesis	14:57	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.
28507244	2	103	theme	cell	495:498	arg1	wall					500:503	the cell wall	491:503	the cell wall	491:503	Oritavancin differs from vancomycin by a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall.
28507244	3	104	theme	liquid	735:740	arg1	LC-MS					776:780	LC-MS	776:780	LC-MS	776:780	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	3	104	theme	liquid	735:740	arg1	spectrometry					762:773	liquid chromatography-mass spectrometry	735:773	liquid chromatography-mass spectrometry (LC-MS)	735:781	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	0	105	theme	Wall	41:44	arg1	Biosynthesis					46:57	Staphylococcus aureus Cell Wall Biosynthesis	14:57	Staphylococcus aureus Cell Wall Biosynthesis	14:57	Inhibition of Staphylococcus aureus Cell Wall Biosynthesis by Desleucyl-Oritavancin: a Quantitative Peptidoglycan Composition Analysis by Mass Spectrometry.
28507244	10	106	theme	glycopeptide	2389:2400	arg1	antibiotics					2402:2412	glycopeptide antibiotics	2389:2412	glycopeptide antibiotics	2389:2412	Our work highlights the use of quantitative peptidoglycan composition analysis by LC-MS to provide insights into the mode of action of glycopeptide antibiotics.
28507244	4	107	theme	due	944:946	arg1	site					939:942	the damaged d-Ala-d-Ala binding site	907:942	the damaged d-Ala-d-Ala binding site due to its functional secondary binding site	907:987	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	2	108	theme	cross-linked	461:472	arg1	peptidoglycan					474:486	the cross-linked peptidoglycan	457:486	the cross-linked peptidoglycan in the cell wall	457:503	Oritavancin differs from vancomycin by a hydrophobic side chain attached to the drug disaccharide, which forms a secondary binding site to enable oritavancin binding to the cross-linked peptidoglycan in the cell wall.
28507244	6	109	theme	peptidoglycan	1646:1658	arg1	structure					1667:1675	the cross-linked peptidoglycan bridge structure	1629:1675	the cross-linked peptidoglycan bridge structure in the cell wall	1629:1692	The changes in peptidoglycan composition suggest that desleucyl-oritavancin targets the peptidoglycan template to induce cell wall disorder and interferes with cell wall maturation.IMPORTANCE Oritavancin is a lipoglycopeptide antibiotic with a secondary binding site that targets the cross-linked peptidoglycan bridge structure in the cell wall.
28507244	9	110	theme	ions	2163:2166	arg1	abundances					2134:2143	the relative abundances	2121:2143	the relative abundances of 83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms	2121:2251	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus was performed by determining the relative abundances of 83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms.
28507244	4	111	theme	damaged	911:917	arg1	site					939:942	the damaged d-Ala-d-Ala binding site	907:942	the damaged d-Ala-d-Ala binding site due to its functional secondary binding site	907:987	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
28507244	3	112	theme	binding	538:544	arg1	site					546:549	secondary binding site	528:549	secondary binding site	528:549	The mode of action of secondary binding site was investigated by measuring the changes in the peptidoglycan composition of Staphylococcus aureus grown in the presence of desleucyl-oritavancin at subinhibitory concentration using liquid chromatography-mass spectrometry (LC-MS).
28507244	7	113	theme	binding	1742:1748	arg1	site					1750:1753	its primary d-Ala-d-Ala binding site	1718:1753	its primary d-Ala-d-Ala binding site through Edman degradation	1718:1779	Even after the loss of its primary d-Ala-d-Ala binding site through Edman degradation, desleucyl-oritavancin exhibits potent antimicrobial activities through its still-functioning secondary binding site.
28507244	5	114	theme	desleucyl-oritavancin	1141:1161	arg1	presence					1129:1136	the presence	1125:1136	the presence of desleucyl-oritavancin	1125:1161	Accurate quantitative peptidoglycan composition analysis based on 83 muropeptide ions determined that cell walls of S. aureus grown in the presence of desleucyl-oritavancin showed a reduction of peptidoglycan cross-linking, increased muropeptides with a tetrapeptide-stem structure, decreased O-acetylation of MurNAc, and increased N-deacetylation of GlcNAc.
28507244	9	115	theme	precalculated	2183:2195	arg1	library					2197:2203	a precalculated library	2181:2203	a precalculated library	2181:2203	Peptidoglycan composition analysis of desleucyl-oritavancin-treated S. aureus was performed by determining the relative abundances of 83 muropeptide ions matched from a precalculated library through integrating extracted ion chromatograms.
28507244	4	116	theme	binding	931:937	arg1	site					939:942	the damaged d-Ala-d-Ala binding site	907:942	the damaged d-Ala-d-Ala binding site due to its functional secondary binding site	907:987	Desleucyl-oritavancin is an Edman degradation product of oritavancin that exhibits potent antibacterial activities despite the damaged d-Ala-d-Ala binding site due to its functional secondary binding site.
27130474	8	0	theme	fibrils	1321:1327	arg1	modulus					1301:1307	the most nonlinearly increasing Young׳s modulus	1261:1307	the most nonlinearly increasing Young׳s modulus of collagen fibrils	1261:1327	The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data.
27130474	8	0	theme	fibrils	1321:1327	arg1	function					1334:1341	a function	1332:1341	a function of strain	1332:1351	The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data.
27130474	4	1	theme	poroelastic	639:649	arg1	models					672:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	11	2	theme	measured	1692:1699	arg1	data					1712:1715	the measured mechanical data	1688:1715	the measured mechanical data	1688:1715	Implementation of the measured volume fractions did not improve the ability of the model to capture the measured mechanical data.
27130474	4	3	from	cartilage	772:780	arg1	indentation					785:795	indentation	785:795	indentation	785:795	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	1	4	theme	proteoglycans	296:308	arg1	composition					222:232	inhomogeneous tissue composition	201:232	inhomogeneous tissue composition	201:232	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	1	4	theme	proteoglycans	296:308	arg1	behavior					274:281	nonlinear mechanical behavior	253:281	nonlinear mechanical behavior of collagen, proteoglycans and fluid	253:318	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	1	4	theme	proteoglycans	296:308	arg1	structure					238:246	structure	238:246	structure	238:246	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	12	5	from	response	1849:1856	arg1	indentation					1891:1901	indentation	1891:1901	indentation with high strain rates	1891:1924	These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.
27130474	4	6	theme	poroviscoelastic	655:670	arg1	models					672:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	10	7	theme	viscoelastic	1542:1553	arg1	fibrils					1564:1570	viscoelastic collagen fibrils	1542:1570	viscoelastic collagen fibrils	1542:1570	Surprisingly, the difference in the peak forces between the experiment and the model with viscoelastic collagen fibrils was almost 20%.
27130474	3	8	theme	nonlinear	591:599	arg1	response					601:608	highly nonlinear response	584:608	highly nonlinear response	584:608	Yet, it is known that in indentation, especially at high strain velocities, cartilage can express highly nonlinear response.
27130474	1	9	theme	fluid	314:318	arg1	composition					222:232	inhomogeneous tissue composition	201:232	inhomogeneous tissue composition	201:232	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	1	9	theme	fluid	314:318	arg1	behavior					274:281	nonlinear mechanical behavior	253:281	nonlinear mechanical behavior of collagen, proteoglycans and fluid	253:318	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	1	9	theme	fluid	314:318	arg1	structure					238:246	structure	238:246	structure	238:246	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	4	10	from	response	743:750	arg1	indentation					785:795	indentation	785:795	indentation	785:795	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	1	11	theme	tissue	215:220	arg1	composition					222:232	inhomogeneous tissue composition	201:232	inhomogeneous tissue composition	201:232	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	10	12	with	forces	1493:1498	arg1	fibrils					1564:1570	viscoelastic collagen fibrils	1542:1570	viscoelastic collagen fibrils	1542:1570	Surprisingly, the difference in the peak forces between the experiment and the model with viscoelastic collagen fibrils was almost 20%.
27130474	12	13	theme	strain	1913:1918	arg1	rates					1920:1924	high strain rates	1908:1924	high strain rates	1908:1924	These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.
27130474	4	14	theme	rabbit	755:760	arg1	cartilage					772:780	rabbit articular cartilage	755:780	rabbit articular cartilage in indentation	755:795	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	2	15	theme	experimental	351:362	arg1	creep					376:380	well experimental single step creep and stress-relaxation tests	346:408	creep	376:380	These models can capture well experimental single step creep and stress-relaxation tests or measurements under small strains in unconfined and confined compression.
27130474	12	16	theme	nonlinear	1754:1762	arg1	formulation					1764:1774	a highly nonlinear formulation	1745:1774	a highly nonlinear formulation for collagen fibrils	1745:1795	These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.
27130474	4	17	theme	measured	699:706	arg1	response					743:750	measured highly nonlinear stress-relaxation response	699:750	measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation	699:795	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	4	18	theme	nonlinear	715:723	arg1	response					743:750	measured highly nonlinear stress-relaxation response	699:750	measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation	699:795	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	7	19	theme	elastic	1212:1218	arg1	representations					1220:1234	multiple nonlinear elastic representations	1193:1234	multiple nonlinear elastic representations	1193:1234	These consisted of linear elastic, nonlinear viscoelastic and multiple nonlinear elastic representations.
27130474	1	20	theme	nonlinear	253:261	arg1	behavior					274:281	nonlinear mechanical behavior	253:281	nonlinear mechanical behavior of collagen, proteoglycans and fluid	253:318	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	10	21	with	model	1531:1535	arg1	fibrils					1564:1570	viscoelastic collagen fibrils	1542:1570	viscoelastic collagen fibrils	1542:1570	Surprisingly, the difference in the peak forces between the experiment and the model with viscoelastic collagen fibrils was almost 20%.
27130474	6	22	theme	different	1068:1076	arg1	equations					1091:1099	five different constitutive equations	1063:1099	five different constitutive equations	1063:1099	In particular, the collagen fibril network was modeled using eight separate models that implemented five different constitutive equations to describe the nonlinearity.
27130474	7	23	theme	multiple	1193:1200	arg1	representations					1220:1234	multiple nonlinear elastic representations	1193:1234	multiple nonlinear elastic representations	1193:1234	These consisted of linear elastic, nonlinear viscoelastic and multiple nonlinear elastic representations.
27130474	1	24	theme	computational	145:157	arg1	models					159:164	Modern fibril-reinforced computational models	120:164	Modern fibril-reinforced computational models of articular cartilage	120:187	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	10	25	from	difference	1470:1479	arg1	forces					1493:1498	the peak forces	1484:1498	the peak forces between the experiment	1484:1521	Surprisingly, the difference in the peak forces between the experiment and the model with viscoelastic collagen fibrils was almost 20%.
27130474	10	25	from	difference	1470:1479	arg1	model					1531:1535	the model	1527:1535	the model with viscoelastic collagen fibrils	1527:1570	Surprisingly, the difference in the peak forces between the experiment and the model with viscoelastic collagen fibrils was almost 20%.
27130474	11	26	theme	mechanical	1701:1710	arg1	data					1712:1715	the measured mechanical data	1688:1715	the measured mechanical data	1688:1715	Implementation of the measured volume fractions did not improve the ability of the model to capture the measured mechanical data.
27130474	12	27	theme	high	1908:1911	arg1	rates					1920:1924	high strain rates	1908:1924	high strain rates	1908:1924	These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.
27130474	4	28	theme	cartilage	772:780	arg1	response					743:750	measured highly nonlinear stress-relaxation response	699:750	measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation	699:795	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	5	29	theme	constituents	875:886	arg1	fractions					845:853	Experimentally measured depth-dependent volume fractions	798:853	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities	798:922	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities were taken into account in the models.
27130474	1	30	theme	cartilage	179:187	arg1	models					159:164	Modern fibril-reinforced computational models	120:164	Modern fibril-reinforced computational models of articular cartilage	120:187	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	12	31	theme	rabbit	1861:1866	arg1	cartilage					1878:1886	rabbit articular cartilage	1861:1886	rabbit articular cartilage	1861:1886	These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.
27130474	12	32	theme	cartilage	1878:1886	arg1	response					1849:1856	multi-step stress-relaxation response	1820:1856	multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates	1820:1924	These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.
27130474	0	33	theme	collagen	97:104	arg1	nonlinearity					106:117	collagen nonlinearity	97:117	collagen nonlinearity	97:117	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.
27130474	12	34	theme	multi-step	1820:1829	arg1	response					1849:1856	multi-step stress-relaxation response	1820:1856	multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates	1820:1924	These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.
27130474	2	35	theme	unconfined	449:458	arg1	compression					473:483	unconfined and confined compression	449:483	unconfined and confined compression	449:483	These models can capture well experimental single step creep and stress-relaxation tests or measurements under small strains in unconfined and confined compression.
27130474	5	36	theme	different	858:866	arg1	constituents					875:886	different tissue constituents	858:886	different tissue constituents	858:886	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities were taken into account in the models.
27130474	0	37	theme	articular	47:55	arg1	cartilage					57:65	articular cartilage	47:65	articular cartilage	47:65	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.
27130474	0	38	theme	stress-relaxation	17:33	arg1	response					35:42	Highly nonlinear stress-relaxation response	0:42	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.	0:118	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.
27130474	6	39	theme	fibril	991:996	arg1	network					998:1004	the collagen fibril network	978:1004	the collagen fibril network	978:1004	In particular, the collagen fibril network was modeled using eight separate models that implemented five different constitutive equations to describe the nonlinearity.
27130474	1	40	theme	Modern	120:125	arg1	models					159:164	Modern fibril-reinforced computational models	120:164	Modern fibril-reinforced computational models of articular cartilage	120:187	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	4	41	theme	Different	611:619	arg1	models					672:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	11	42	theme	volume	1619:1624	arg1	fractions					1626:1634	the measured volume fractions	1606:1634	the measured volume fractions	1606:1634	Implementation of the measured volume fractions did not improve the ability of the model to capture the measured mechanical data.
27130474	2	43	theme	confined	464:471	arg1	compression					473:483	unconfined and confined compression	449:483	unconfined and confined compression	449:483	These models can capture well experimental single step creep and stress-relaxation tests or measurements under small strains in unconfined and confined compression.
27130474	5	44	theme	nonlinearities	909:922	arg1	fractions					845:853	Experimentally measured depth-dependent volume fractions	798:853	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities	798:922	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities were taken into account in the models.
27130474	2	45	from	strains	438:444	arg1	compression					473:483	unconfined and confined compression	449:483	unconfined and confined compression	449:483	These models can capture well experimental single step creep and stress-relaxation tests or measurements under small strains in unconfined and confined compression.
27130474	2	46	theme	stress-relaxation	386:402	arg1	tests					404:408	well experimental single step creep and stress-relaxation tests	346:408	tests	404:408	These models can capture well experimental single step creep and stress-relaxation tests or measurements under small strains in unconfined and confined compression.
27130474	0	47	theme	cartilage	57:65	arg1	response					35:42	Highly nonlinear stress-relaxation response	0:42	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.	0:118	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.
27130474	10	48	theme	peak	1488:1491	arg1	forces					1493:1498	the peak forces	1484:1498	the peak forces between the experiment	1484:1521	Surprisingly, the difference in the peak forces between the experiment and the model with viscoelastic collagen fibrils was almost 20%.
27130474	1	49	theme	mechanical	263:272	arg1	behavior					274:281	nonlinear mechanical behavior	253:281	nonlinear mechanical behavior of collagen, proteoglycans and fluid	253:318	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	8	50	theme	increasing	1282:1291	arg1	modulus					1301:1307	the most nonlinearly increasing Young׳s modulus	1261:1307	the most nonlinearly increasing Young׳s modulus of collagen fibrils	1261:1327	The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data.
27130474	8	50	theme	increasing	1282:1291	arg1	function					1334:1341	a function	1332:1341	a function of strain	1332:1351	The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data.
27130474	9	51	dep	model	1422:1426	arg1	the					1418:1420	the	1418:1420	the	1418:1420	Relative difference between the model and experiment was ~3%.
27130474	3	52	theme	strain	543:548	arg1	velocities					550:559	high strain velocities	538:559	high strain velocities	538:559	Yet, it is known that in indentation, especially at high strain velocities, cartilage can express highly nonlinear response.
27130474	8	53	theme	collagen	1312:1319	arg1	fibrils					1321:1327	collagen fibrils	1312:1327	collagen fibrils	1312:1327	The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data.
27130474	6	54	theme	separate	1030:1037	arg1	models					1039:1044	eight separate models	1024:1044	eight separate models that implemented five different constitutive equations to describe the nonlinearity	1024:1128	In particular, the collagen fibril network was modeled using eight separate models that implemented five different constitutive equations to describe the nonlinearity.
27130474	4	55	theme	reinforced	628:637	arg1	models					672:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	10	56	theme	collagen	1555:1562	arg1	fibrils					1564:1570	viscoelastic collagen fibrils	1542:1570	viscoelastic collagen fibrils	1542:1570	Surprisingly, the difference in the peak forces between the experiment and the model with viscoelastic collagen fibrils was almost 20%.
27130474	1	57	theme	inhomogeneous	201:213	arg1	composition					222:232	inhomogeneous tissue composition	201:232	inhomogeneous tissue composition	201:232	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	4	58	theme	stress-relaxation	725:741	arg1	response					743:750	measured highly nonlinear stress-relaxation response	699:750	measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation	699:795	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	2	59	theme	single	364:369	arg1	creep					376:380	well experimental single step creep and stress-relaxation tests	346:408	creep	376:380	These models can capture well experimental single step creep and stress-relaxation tests or measurements under small strains in unconfined and confined compression.
27130474	8	60	theme	strain	1346:1351	arg1	modulus					1301:1307	the most nonlinearly increasing Young׳s modulus	1261:1307	the most nonlinearly increasing Young׳s modulus of collagen fibrils	1261:1327	The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data.
27130474	8	60	theme	strain	1346:1351	arg1	function					1334:1341	a function	1332:1341	a function of strain	1332:1351	The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data.
27130474	4	61	used	used	684:687	arg2	models					672:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	9	62	theme	Relative	1390:1397	arg1	difference					1399:1408	Relative difference	1390:1408	Relative difference between the model and experiment	1390:1441	Relative difference between the model and experiment was ~3%.
27130474	8	63	theme	best	1362:1365	arg1	data					1384:1387	the experimental data	1367:1387	best the experimental data	1362:1387	The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data.
27130474	12	64	theme	collagen	1780:1787	arg1	fibrils					1789:1795	collagen fibrils	1780:1795	collagen fibrils	1780:1795	These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.
27130474	7	65	theme	nonlinear	1202:1210	arg1	representations					1220:1234	multiple nonlinear elastic representations	1193:1234	multiple nonlinear elastic representations	1193:1234	These consisted of linear elastic, nonlinear viscoelastic and multiple nonlinear elastic representations.
27130474	8	66	theme	experimental	1371:1382	arg1	data					1384:1387	the experimental data	1367:1387	best the experimental data	1362:1387	The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data.
27130474	6	67	theme	constitutive	1078:1089	arg1	equations					1091:1099	five different constitutive equations	1063:1099	five different constitutive equations	1063:1099	In particular, the collagen fibril network was modeled using eight separate models that implemented five different constitutive equations to describe the nonlinearity.
27130474	5	68	theme	volume	838:843	arg1	fractions					845:853	Experimentally measured depth-dependent volume fractions	798:853	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities	798:922	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities were taken into account in the models.
27130474	1	69	theme	fibril-reinforced	127:143	arg1	models					159:164	Modern fibril-reinforced computational models	120:164	Modern fibril-reinforced computational models of articular cartilage	120:187	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	5	70	theme	tissue	868:873	arg1	constituents					875:886	different tissue constituents	858:886	different tissue constituents	858:886	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities were taken into account in the models.
27130474	9	71	theme	~3	1447:1448	arg1	%					1449:1449	~3%	1447:1449	~3%	1447:1449	Relative difference between the model and experiment was ~3%.
27130474	1	72	theme	articular	169:177	arg1	cartilage					179:187	articular cartilage	169:187	articular cartilage	169:187	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	0	73	from	response	35:42	arg1	indentation					70:80	indentation	70:80	indentation	70:80	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.
27130474	4	74	theme	articular	762:770	arg1	cartilage					772:780	rabbit articular cartilage	755:780	rabbit articular cartilage in indentation	755:795	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	11	75	theme	model	1671:1675	arg1	ability					1656:1662	the ability	1652:1662	the ability of the model to capture the measured mechanical data	1652:1715	Implementation of the measured volume fractions did not improve the ability of the model to capture the measured mechanical data.
27130474	0	76	theme	nonlinearity	106:117	arg1	Importance					83:92	Importance	83:92	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.	0:118	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.
27130474	12	77	theme	articular	1868:1876	arg1	cartilage					1878:1886	rabbit articular cartilage	1861:1886	rabbit articular cartilage	1861:1886	These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.
27130474	12	78	with	indentation	1891:1901	arg1	rates					1920:1924	high strain rates	1908:1924	high strain rates	1908:1924	These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.
27130474	0	79	theme	nonlinear	7:15	arg1	response					35:42	Highly nonlinear stress-relaxation response	0:42	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.	0:118	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.
27130474	12	80	theme	stress-relaxation	1831:1847	arg1	response					1849:1856	multi-step stress-relaxation response	1820:1856	multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates	1820:1924	These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.
27130474	5	81	theme	measured	813:820	arg1	fractions					845:853	Experimentally measured depth-dependent volume fractions	798:853	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities	798:922	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities were taken into account in the models.
27130474	11	82	theme	fractions	1626:1634	arg1	Implementation					1588:1601	Implementation	1588:1601	Implementation of the measured volume fractions	1588:1634	Implementation of the measured volume fractions did not improve the ability of the model to capture the measured mechanical data.
27130474	2	83	theme	small	432:436	arg1	strains					438:444	small strains	432:444	small strains in unconfined and confined compression	432:483	These models can capture well experimental single step creep and stress-relaxation tests or measurements under small strains in unconfined and confined compression.
27130474	4	84	theme	fibril	621:626	arg1	models					672:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models	611:677	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	11	85	theme	measured	1610:1617	arg1	fractions					1626:1634	the measured volume fractions	1606:1634	the measured volume fractions	1606:1634	Implementation of the measured volume fractions did not improve the ability of the model to capture the measured mechanical data.
27130474	5	86	theme	mechanical	898:907	arg1	nonlinearities					909:922	their mechanical nonlinearities	892:922	their mechanical nonlinearities	892:922	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities were taken into account in the models.
27130474	6	87	theme	collagen	982:989	arg1	network					998:1004	the collagen fibril network	978:1004	the collagen fibril network	978:1004	In particular, the collagen fibril network was modeled using eight separate models that implemented five different constitutive equations to describe the nonlinearity.
27130474	5	88	theme	depth-dependent	822:836	arg1	fractions					845:853	Experimentally measured depth-dependent volume fractions	798:853	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities	798:922	Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities were taken into account in the models.
27130474	3	89	theme	high	538:541	arg1	velocities					550:559	high strain velocities	538:559	high strain velocities	538:559	Yet, it is known that in indentation, especially at high strain velocities, cartilage can express highly nonlinear response.
27130474	8	90	theme	Young׳s	1293:1299	arg1	modulus					1301:1307	the most nonlinearly increasing Young׳s modulus	1261:1307	the most nonlinearly increasing Young׳s modulus of collagen fibrils	1261:1327	The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data.
27130474	8	90	theme	Young׳s	1293:1299	arg1	function					1334:1341	a function	1332:1341	a function of strain	1332:1351	The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data.
27130474	0	91	dep	response	35:42	arg1	Importance					83:92	Importance	83:92	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.	0:118	Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.
27130474	2	92	theme	step	371:374	arg1	creep					376:380	well experimental single step creep and stress-relaxation tests	346:408	creep	376:380	These models can capture well experimental single step creep and stress-relaxation tests or measurements under small strains in unconfined and confined compression.
27130474	4	93	from	indentation	785:795	arg1	response					743:750	measured highly nonlinear stress-relaxation response	699:750	measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation	699:795	Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation.
27130474	1	94	theme	collagen	286:293	arg1	composition					222:232	inhomogeneous tissue composition	201:232	inhomogeneous tissue composition	201:232	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	1	94	theme	collagen	286:293	arg1	behavior					274:281	nonlinear mechanical behavior	253:281	nonlinear mechanical behavior of collagen, proteoglycans and fluid	253:318	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
27130474	1	94	theme	collagen	286:293	arg1	structure					238:246	structure	238:246	structure	238:246	Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid.
25193152	11	0	theme	mediated	1542:1549	arg1	pathway					1551:1557	the clathrin mediated pathway	1529:1557	the clathrin mediated pathway	1529:1557	The results revealed that the clathrin mediated pathway and dynamin played a role in the internalisation of these specific nanoparticles.
25193152	6	1	theme	charged	920:926	arg1	DNA					928:930	negatively charged DNA	909:930	negatively charged DNA	909:930	The cationic derivative formed nanoparticles when mixed with negatively charged DNA.
25193152	3	2	with	conjugation	502:512	arg1	ornithine					534:542	the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery	519:607	the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery	519:607	In this study, chitosan, a versatile biopolymer has been subjected to chemical conjugation with the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery.
25193152	7	3	theme	agarose	990:996	arg1	electrophoresis					1002:1016	agarose gel electrophoresis	990:1016	agarose gel electrophoresis	990:1016	The nanoparticles showed good DNA retardation ability in agarose gel electrophoresis and sizes were ascertained by DLS and TEM observations.
25193152	1	4	theme	gene	175:178	arg1	therapy					180:186	gene therapy	175:186	gene therapy research	175:195	Nanotechnology is adopted in gene therapy research to create gene vectors that will facilitate gene transfer to cells with utmost efficacy and safety.
25193152	8	5	from	aggregation	1201:1211	arg1	blood					1216:1220	blood	1216:1220	blood	1216:1220	The derivative on interaction with blood plasma showed negligible protein adsorption and did not cause either hemolysis or RBC aggregation in blood.
25193152	7	6	theme	retardation	967:977	arg1	ability					979:985	good DNA retardation ability	958:985	good DNA retardation ability	958:985	The nanoparticles showed good DNA retardation ability in agarose gel electrophoresis and sizes were ascertained by DLS and TEM observations.
25193152	1	7	theme	therapy	180:186	arg1	research					188:195	gene therapy research	175:195	gene therapy research	175:195	Nanotechnology is adopted in gene therapy research to create gene vectors that will facilitate gene transfer to cells with utmost efficacy and safety.
25193152	1	8	theme	utmost	269:274	arg1	efficacy					276:283	utmost efficacy	269:283	utmost efficacy	269:283	Nanotechnology is adopted in gene therapy research to create gene vectors that will facilitate gene transfer to cells with utmost efficacy and safety.
25193152	10	9	theme	inhibitors	1440:1449	arg1	presence					1416:1423	the presence	1412:1423	the presence of endocytosis inhibitors	1412:1449	An uptake study in the presence of endocytosis inhibitors indicated the specific pathway used for cell entry.
25193152	10	10	theme	uptake	1396:1401	arg1	study					1403:1407	An uptake study	1393:1407	An uptake study in the presence of endocytosis inhibitors	1393:1449	An uptake study in the presence of endocytosis inhibitors indicated the specific pathway used for cell entry.
25193152	2	11	theme	vector	301:306	arg1	design					308:313	vector design	301:313	vector design	301:313	For vector design, polymers are the preferred nonviral colloidal systems as they are feasible for any chemical modifications.
25193152	9	12	dep	In	1223:1224	arg1	vitro					1226:1230	vitro	1226:1230	vitro	1226:1230	In vitro cell culture also revealed the CON derivative to be nontoxic to cells and capable of transfection with an explicit increase in cellular uptake of nanoparticles.
25193152	0	13	theme	chitosan	107:114	arg1	polycation					116:125	chitosan polycation	107:125	chitosan polycation for gene delivery	107:143	Enhanced intracellular uptake and endocytic pathway selection mediated by hemocompatible ornithine grafted chitosan polycation for gene delivery.
25193152	10	14	theme	endocytosis	1428:1438	arg1	inhibitors					1440:1449	endocytosis inhibitors	1428:1449	endocytosis inhibitors	1428:1449	An uptake study in the presence of endocytosis inhibitors indicated the specific pathway used for cell entry.
25193152	7	15	theme	good	958:961	arg1	ability					979:985	good DNA retardation ability	958:985	good DNA retardation ability	958:985	The nanoparticles showed good DNA retardation ability in agarose gel electrophoresis and sizes were ascertained by DLS and TEM observations.
25193152	11	16	theme	specific	1617:1624	arg1	nanoparticles					1626:1638	these specific nanoparticles	1611:1638	these specific nanoparticles	1611:1638	The results revealed that the clathrin mediated pathway and dynamin played a role in the internalisation of these specific nanoparticles.
25193152	0	17	theme	gene	131:134	arg1	delivery					136:143	gene delivery	131:143	gene delivery	131:143	Enhanced intracellular uptake and endocytic pathway selection mediated by hemocompatible ornithine grafted chitosan polycation for gene delivery.
25193152	9	18	from	increase	1347:1354	arg1	uptake					1368:1373	cellular uptake	1359:1373	cellular uptake of nanoparticles	1359:1390	In vitro cell culture also revealed the CON derivative to be nontoxic to cells and capable of transfection with an explicit increase in cellular uptake of nanoparticles.
25193152	7	19	theme	DNA	963:965	arg1	ability					979:985	good DNA retardation ability	958:985	good DNA retardation ability	958:985	The nanoparticles showed good DNA retardation ability in agarose gel electrophoresis and sizes were ascertained by DLS and TEM observations.
25193152	3	20	theme	acid	529:532	arg1	ornithine					534:542	the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery	519:607	the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery	519:607	In this study, chitosan, a versatile biopolymer has been subjected to chemical conjugation with the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery.
25193152	2	21	theme	chemical	399:406	arg1	modifications					408:420	any chemical modifications	395:420	any chemical modifications	395:420	For vector design, polymers are the preferred nonviral colloidal systems as they are feasible for any chemical modifications.
25193152	5	22	theme	Buffering	720:728	arg1	capacity					730:737	Buffering capacity	720:737	Buffering capacity	720:737	Buffering capacity was found enhanced with the synthesised chitosan derivative when compared to the parent unmodified chitosan.
25193152	9	23	theme	transfection	1317:1328	arg1	capable					1306:1312	capable	1306:1312	capable	1306:1312	In vitro cell culture also revealed the CON derivative to be nontoxic to cells and capable of transfection with an explicit increase in cellular uptake of nanoparticles.
25193152	8	24	theme	RBC	1197:1199	arg1	aggregation					1201:1211	RBC aggregation	1197:1211	RBC aggregation in blood	1197:1220	The derivative on interaction with blood plasma showed negligible protein adsorption and did not cause either hemolysis or RBC aggregation in blood.
25193152	4	25	theme	1	637:637	arg1	spectra					645:651	FTIR and (1)H NMR spectra	627:651	spectra	645:651	With the help of FTIR and (1)H NMR spectra the chemical composition of the chitosan derivative was confirmed.
25193152	0	26	theme	intracellular	9:21	arg1	uptake					23:28	Enhanced intracellular uptake	0:28	Enhanced intracellular uptake	0:28	Enhanced intracellular uptake and endocytic pathway selection mediated by hemocompatible ornithine grafted chitosan polycation for gene delivery.
25193152	9	27	theme	In	1223:1224	arg1	culture					1237:1243	In vitro cell culture	1223:1243	In vitro cell culture	1223:1243	In vitro cell culture also revealed the CON derivative to be nontoxic to cells and capable of transfection with an explicit increase in cellular uptake of nanoparticles.
25193152	4	28	theme	NMR	641:643	arg1	spectra					645:651	FTIR and (1)H NMR spectra	627:651	spectra	645:651	With the help of FTIR and (1)H NMR spectra the chemical composition of the chitosan derivative was confirmed.
25193152	0	29	theme	Enhanced	0:7	arg1	uptake					23:28	Enhanced intracellular uptake	0:28	Enhanced intracellular uptake	0:28	Enhanced intracellular uptake and endocytic pathway selection mediated by hemocompatible ornithine grafted chitosan polycation for gene delivery.
25193152	10	30	theme	specific	1465:1472	arg1	pathway					1474:1480	the specific pathway	1461:1480	the specific pathway used for cell entry	1461:1500	An uptake study in the presence of endocytosis inhibitors indicated the specific pathway used for cell entry.
25193152	11	31	theme	nanoparticles	1626:1638	arg1	internalisation					1592:1606	the internalisation	1588:1606	the internalisation of these specific nanoparticles	1588:1638	The results revealed that the clathrin mediated pathway and dynamin played a role in the internalisation of these specific nanoparticles.
25193152	10	32	from	study	1403:1407	arg1	presence					1416:1423	the presence	1412:1423	the presence of endocytosis inhibitors	1412:1449	An uptake study in the presence of endocytosis inhibitors indicated the specific pathway used for cell entry.
25193152	1	33	theme	gene	207:210	arg1	vectors					212:218	gene vectors	207:218	gene vectors that will facilitate gene transfer to cells with utmost efficacy and safety	207:294	Nanotechnology is adopted in gene therapy research to create gene vectors that will facilitate gene transfer to cells with utmost efficacy and safety.
25193152	9	34	theme	explicit	1338:1345	arg1	increase					1347:1354	an explicit increase	1335:1354	an explicit increase in cellular uptake of nanoparticles	1335:1390	In vitro cell culture also revealed the CON derivative to be nontoxic to cells and capable of transfection with an explicit increase in cellular uptake of nanoparticles.
25193152	5	35	dep	found	743:747	arg1	enhanced					749:756	enhanced	749:756	enhanced with the synthesised chitosan derivative when compared to the parent unmodified chitosan	749:845	Buffering capacity was found enhanced with the synthesised chitosan derivative when compared to the parent unmodified chitosan.
25193152	9	36	theme	cell	1232:1235	arg1	culture					1237:1243	In vitro cell culture	1223:1243	In vitro cell culture	1223:1243	In vitro cell culture also revealed the CON derivative to be nontoxic to cells and capable of transfection with an explicit increase in cellular uptake of nanoparticles.
25193152	3	37	theme	chitosan-ornithine	556:573	arg1	conjugate					575:583	chitosan-ornithine conjugate	556:583	chitosan-ornithine conjugate (CON) for gene delivery	556:607	In this study, chitosan, a versatile biopolymer has been subjected to chemical conjugation with the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery.
25193152	3	37	theme	chitosan-ornithine	556:573	arg1	CON					586:588	CON	586:588	CON	586:588	In this study, chitosan, a versatile biopolymer has been subjected to chemical conjugation with the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery.
25193152	4	38	theme	FTIR	627:630	arg1	spectra					645:651	FTIR and (1)H NMR spectra	627:651	spectra	645:651	With the help of FTIR and (1)H NMR spectra the chemical composition of the chitosan derivative was confirmed.
25193152	4	39	theme	chemical	657:664	arg1	composition					666:676	the chemical composition	653:676	the chemical composition of the chitosan derivative	653:703	With the help of FTIR and (1)H NMR spectra the chemical composition of the chitosan derivative was confirmed.
25193152	5	40	theme	parent	820:825	arg1	chitosan					838:845	the parent unmodified chitosan	816:845	the parent unmodified chitosan	816:845	Buffering capacity was found enhanced with the synthesised chitosan derivative when compared to the parent unmodified chitosan.
25193152	0	41	theme	pathway	44:50	arg1	selection					52:60	endocytic pathway selection	34:60	endocytic pathway selection	34:60	Enhanced intracellular uptake and endocytic pathway selection mediated by hemocompatible ornithine grafted chitosan polycation for gene delivery.
25193152	3	42	theme	chemical	493:500	arg1	conjugation					502:512	chemical conjugation	493:512	chemical conjugation with the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery	493:607	In this study, chitosan, a versatile biopolymer has been subjected to chemical conjugation with the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery.
25193152	8	43	theme	blood	1109:1113	arg1	plasma					1115:1120	blood plasma	1109:1120	blood plasma	1109:1120	The derivative on interaction with blood plasma showed negligible protein adsorption and did not cause either hemolysis or RBC aggregation in blood.
25193152	6	44	theme	cationic	852:859	arg1	derivative					861:870	The cationic derivative	848:870	The cationic derivative formed	848:877	The cationic derivative formed nanoparticles when mixed with negatively charged DNA.
25193152	3	45	theme	gene	595:598	arg1	delivery					600:607	gene delivery	595:607	gene delivery	595:607	In this study, chitosan, a versatile biopolymer has been subjected to chemical conjugation with the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery.
25193152	4	46	theme	H	639:639	arg1	spectra					645:651	FTIR and (1)H NMR spectra	627:651	spectra	645:651	With the help of FTIR and (1)H NMR spectra the chemical composition of the chitosan derivative was confirmed.
25193152	5	47	theme	unmodified	827:836	arg1	chitosan					838:845	the parent unmodified chitosan	816:845	the parent unmodified chitosan	816:845	Buffering capacity was found enhanced with the synthesised chitosan derivative when compared to the parent unmodified chitosan.
25193152	0	48	theme	endocytic	34:42	arg1	selection					52:60	endocytic pathway selection	34:60	endocytic pathway selection	34:60	Enhanced intracellular uptake and endocytic pathway selection mediated by hemocompatible ornithine grafted chitosan polycation for gene delivery.
25193152	8	49	from	derivative	1078:1087	arg1	interaction					1092:1102	interaction	1092:1102	interaction with blood plasma	1092:1120	The derivative on interaction with blood plasma showed negligible protein adsorption and did not cause either hemolysis or RBC aggregation in blood.
25193152	10	50	theme	cell	1491:1494	arg1	entry					1496:1500	cell entry	1491:1500	cell entry	1491:1500	An uptake study in the presence of endocytosis inhibitors indicated the specific pathway used for cell entry.
25193152	3	51	theme	versatile	450:458	arg1	chitosan					438:445	chitosan	438:445	chitosan	438:445	In this study, chitosan, a versatile biopolymer has been subjected to chemical conjugation with the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery.
25193152	3	51	theme	versatile	450:458	arg1	biopolymer					460:469	a versatile biopolymer	448:469	a versatile biopolymer	448:469	In this study, chitosan, a versatile biopolymer has been subjected to chemical conjugation with the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery.
25193152	5	52	theme	synthesised	767:777	arg1	derivative					788:797	the synthesised chitosan derivative	763:797	the synthesised chitosan derivative	763:797	Buffering capacity was found enhanced with the synthesised chitosan derivative when compared to the parent unmodified chitosan.
25193152	9	53	theme	cellular	1359:1366	arg1	uptake					1368:1373	cellular uptake	1359:1373	cellular uptake of nanoparticles	1359:1390	In vitro cell culture also revealed the CON derivative to be nontoxic to cells and capable of transfection with an explicit increase in cellular uptake of nanoparticles.
25193152	2	54	theme	colloidal	352:360	arg1	polymers					316:323	polymers	316:323	polymers	316:323	For vector design, polymers are the preferred nonviral colloidal systems as they are feasible for any chemical modifications.
25193152	2	54	theme	colloidal	352:360	arg1	systems					362:368	the preferred nonviral colloidal systems	329:368	the preferred nonviral colloidal systems	329:368	For vector design, polymers are the preferred nonviral colloidal systems as they are feasible for any chemical modifications.
25193152	5	55	theme	chitosan	779:786	arg1	derivative					788:797	the synthesised chitosan derivative	763:797	the synthesised chitosan derivative	763:797	Buffering capacity was found enhanced with the synthesised chitosan derivative when compared to the parent unmodified chitosan.
25193152	7	56	theme	TEM	1056:1058	arg1	observations					1060:1071	TEM observations	1056:1071	TEM observations	1056:1071	The nanoparticles showed good DNA retardation ability in agarose gel electrophoresis and sizes were ascertained by DLS and TEM observations.
25193152	9	57	theme	CON	1263:1265	arg1	derivative					1267:1276	the CON derivative	1259:1276	the CON derivative to be nontoxic to cells and capable of transfection with an explicit increase in cellular uptake of nanoparticles	1259:1390	In vitro cell culture also revealed the CON derivative to be nontoxic to cells and capable of transfection with an explicit increase in cellular uptake of nanoparticles.
25193152	8	58	from	hemolysis	1184:1192	arg1	blood					1216:1220	blood	1216:1220	blood	1216:1220	The derivative on interaction with blood plasma showed negligible protein adsorption and did not cause either hemolysis or RBC aggregation in blood.
25193152	2	59	theme	nonviral	343:350	arg1	polymers					316:323	polymers	316:323	polymers	316:323	For vector design, polymers are the preferred nonviral colloidal systems as they are feasible for any chemical modifications.
25193152	2	59	theme	nonviral	343:350	arg1	systems					362:368	the preferred nonviral colloidal systems	329:368	the preferred nonviral colloidal systems	329:368	For vector design, polymers are the preferred nonviral colloidal systems as they are feasible for any chemical modifications.
25193152	9	60	with	transfection	1317:1328	arg1	increase					1347:1354	an explicit increase	1335:1354	an explicit increase in cellular uptake of nanoparticles	1335:1390	In vitro cell culture also revealed the CON derivative to be nontoxic to cells and capable of transfection with an explicit increase in cellular uptake of nanoparticles.
25193152	9	61	theme	nanoparticles	1378:1390	arg1	uptake					1368:1373	cellular uptake	1359:1373	cellular uptake of nanoparticles	1359:1390	In vitro cell culture also revealed the CON derivative to be nontoxic to cells and capable of transfection with an explicit increase in cellular uptake of nanoparticles.
25193152	8	62	theme	protein	1140:1146	arg1	adsorption					1148:1157	negligible protein adsorption	1129:1157	negligible protein adsorption	1129:1157	The derivative on interaction with blood plasma showed negligible protein adsorption and did not cause either hemolysis or RBC aggregation in blood.
25193152	4	63	dep	the	615:617	arg1	help					619:622	help	619:622	help	619:622	With the help of FTIR and (1)H NMR spectra the chemical composition of the chitosan derivative was confirmed.
25193152	2	64	theme	preferred	333:341	arg1	polymers					316:323	polymers	316:323	polymers	316:323	For vector design, polymers are the preferred nonviral colloidal systems as they are feasible for any chemical modifications.
25193152	2	64	theme	preferred	333:341	arg1	systems					362:368	the preferred nonviral colloidal systems	329:368	the preferred nonviral colloidal systems	329:368	For vector design, polymers are the preferred nonviral colloidal systems as they are feasible for any chemical modifications.
25193152	1	65	theme	gene	241:244	arg1	transfer					246:253	gene transfer	241:253	gene transfer to cells with utmost efficacy and safety	241:294	Nanotechnology is adopted in gene therapy research to create gene vectors that will facilitate gene transfer to cells with utmost efficacy and safety.
25193152	8	66	theme	negligible	1129:1138	arg1	adsorption					1148:1157	negligible protein adsorption	1129:1157	negligible protein adsorption	1129:1157	The derivative on interaction with blood plasma showed negligible protein adsorption and did not cause either hemolysis or RBC aggregation in blood.
25193152	7	67	theme	gel	998:1000	arg1	electrophoresis					1002:1016	agarose gel electrophoresis	990:1016	agarose gel electrophoresis	990:1016	The nanoparticles showed good DNA retardation ability in agarose gel electrophoresis and sizes were ascertained by DLS and TEM observations.
25193152	4	68	theme	chitosan	685:692	arg1	derivative					694:703	the chitosan derivative	681:703	the chitosan derivative	681:703	With the help of FTIR and (1)H NMR spectra the chemical composition of the chitosan derivative was confirmed.
25193152	8	69	with	interaction	1092:1102	arg1	plasma					1115:1120	blood plasma	1109:1120	blood plasma	1109:1120	The derivative on interaction with blood plasma showed negligible protein adsorption and did not cause either hemolysis or RBC aggregation in blood.
25193152	4	70	theme	derivative	694:703	arg1	composition					666:676	the chemical composition	653:676	the chemical composition of the chitosan derivative	653:703	With the help of FTIR and (1)H NMR spectra the chemical composition of the chitosan derivative was confirmed.
25193152	0	71	theme	hemocompatible	74:87	arg1	ornithine					89:97	hemocompatible ornithine	74:97	hemocompatible ornithine	74:97	Enhanced intracellular uptake and endocytic pathway selection mediated by hemocompatible ornithine grafted chitosan polycation for gene delivery.
25193152	1	72	with	cells	258:262	arg1	safety					289:294	safety	289:294	safety	289:294	Nanotechnology is adopted in gene therapy research to create gene vectors that will facilitate gene transfer to cells with utmost efficacy and safety.
25193152	1	72	with	cells	258:262	arg1	efficacy					276:283	utmost efficacy	269:283	utmost efficacy	269:283	Nanotechnology is adopted in gene therapy research to create gene vectors that will facilitate gene transfer to cells with utmost efficacy and safety.
25193152	3	73	theme	amino	523:527	arg1	ornithine					534:542	the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery	519:607	the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery	519:607	In this study, chitosan, a versatile biopolymer has been subjected to chemical conjugation with the amino acid ornithine to generate chitosan-ornithine conjugate (CON) for gene delivery.
25462921	1	0	theme	flour	407:411	arg1	reduction					224:232	the reduction	220:232	the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein	220:367	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	0	theme	flour	407:411	arg1	digestibility					380:392	starch digestibility	373:392	starch digestibility of faba bean flour	373:411	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	4	1	from	growth	668:673	arg1	fractions					720:728	its fractions	716:728	its fractions	716:728	Lactobacillus plantarum growth and acidification in faba bean flour and its fractions were assessed.
25462921	4	1	from	growth	668:673	arg1	flour					706:710	faba bean flour	696:710	faba bean flour	696:710	Lactobacillus plantarum growth and acidification in faba bean flour and its fractions were assessed.
25462921	1	2	theme	anti-nutritional	237:252	arg1	convicine					274:282	convicine	274:282	convicine	274:282	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	2	theme	anti-nutritional	237:252	arg1	activity					303:310	trypsin inhibitor activity	285:310	trypsin inhibitor activity	285:310	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	2	theme	anti-nutritional	237:252	arg1	vicine					263:268	vicine	263:268	vicine	263:268	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	2	theme	anti-nutritional	237:252	arg1	factors					254:260	anti-nutritional factors	237:260	anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid)	237:346	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	2	theme	anti-nutritional	237:252	arg1	acid					342:345	phytic acid	335:345	phytic acid	335:345	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	9	3	theme	nutritional	1415:1425	arg1	functionality					1427:1439	nutritional functionality	1415:1439	nutritional functionality of faba bean flour which could be utilized in various food applications	1415:1511	This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.
25462921	6	4	theme	contents	890:897	arg1	decrease					857:864	the decrease	853:864	the decrease of vicine and convicine contents	853:897	Fermentation caused the decrease of vicine and convicine contents by more than 91% and significantly reduced trypsin inhibitor activity and condensed tannins (by more than 40% in the protein-rich fraction).
25462921	8	5	theme	acids	1206:1210	arg1	FAA					1167:1169	FAA	1167:1169	FAA	1167:1169	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	8	5	theme	acids	1206:1210	arg1	amount					1157:1162	the amount	1153:1162	the amount	1153:1162	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	8	5	theme	acids	1206:1210	arg1	acids					1206:1210	the essential amino acids	1186:1210	the essential amino acids	1186:1210	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	8	5	theme	acids	1206:1210	arg1	acid					1231:1234	γ-aminobutyric acid	1216:1234	γ-aminobutyric acid	1216:1234	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	3	6	theme	Air	488:490	arg1	classification					492:505	Air classification	488:505	Air classification	488:505	Air classification allowed the separation of the flour into protein and starch rich fractions, showing different chemical compositions and microstructures.
25462921	4	7	theme	Lactobacillus	644:656	arg1	growth					668:673	Lactobacillus plantarum growth	644:673	Lactobacillus plantarum growth	644:673	Lactobacillus plantarum growth and acidification in faba bean flour and its fractions were assessed.
25462921	9	8	theme	bean	1449:1452	arg1	flour					1454:1458	faba bean flour	1444:1458	faba bean flour	1444:1458	This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.
25462921	0	9	theme	faba	106:109	arg1	bean					94:97	faba bean	89:97	faba bean (Vicia faba L.) flour nutritional properties	89:142	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	0	9	theme	faba	106:109	arg1	L.					111:112	Vicia faba L.	100:112	Vicia faba L.	100:112	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	1	10	theme	lactic	183:188	arg1	bacteria					195:202	lactic acid bacteria	183:202	lactic acid bacteria	183:202	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	6	11	theme	inhibitor	950:958	arg1	activity					960:967	trypsin inhibitor activity	942:967	trypsin inhibitor activity	942:967	Fermentation caused the decrease of vicine and convicine contents by more than 91% and significantly reduced trypsin inhibitor activity and condensed tannins (by more than 40% in the protein-rich fraction).
25462921	9	12	theme	fermentation	1393:1404	arg1	combination					1355:1365	the combination	1351:1365	the combination of air classification and fermentation	1351:1404	This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.
25462921	5	13	theme	fine	805:808	arg1	fraction					823:830	the fine protein-rich fraction	801:830	the fine protein-rich fraction	801:830	The anti-nutritional compounds were separated mostly to the fine protein-rich fraction.
25462921	1	14	theme	bacteria	195:202	arg1	effects					149:155	The effects	145:155	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour	145:411	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	8	15	theme	essential	1190:1198	arg1	acids					1206:1210	the essential amino acids	1186:1210	the essential amino acids	1186:1210	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	4	16	theme	faba	696:699	arg1	flour					706:710	faba bean flour	696:710	faba bean flour	696:710	Lactobacillus plantarum growth and acidification in faba bean flour and its fractions were assessed.
25462921	0	17	theme	nutritional	121:131	arg1	properties					133:142	faba bean (Vicia faba L.) flour nutritional properties	89:142	faba bean (Vicia faba L.) flour nutritional properties	89:142	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	1	18	theme	faba	397:400	arg1	flour					407:411	faba bean flour	397:411	faba bean flour	397:411	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	9	19	theme	food	1495:1498	arg1	applications					1500:1511	various food applications	1487:1511	various food applications	1487:1511	This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.
25462921	0	20	theme	plantarum	63:71	arg1	E-133328					77:84	Lactobacillus plantarum VTT E-133328	49:84	Lactobacillus plantarum VTT E-133328	49:84	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	1	21	theme	condensed	313:321	arg1	tannins					323:329	condensed tannins	313:329	condensed tannins	313:329	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	5	22	theme	protein-rich	810:821	arg1	fraction					823:830	the fine protein-rich fraction	801:830	the fine protein-rich fraction	801:830	The anti-nutritional compounds were separated mostly to the fine protein-rich fraction.
25462921	1	23	from	effects	149:155	arg1	reduction					224:232	the reduction	220:232	the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein	220:367	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	23	from	effects	149:155	arg1	digestibility					380:392	starch digestibility	373:392	starch digestibility of faba bean flour	373:411	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	5	24	theme	anti-nutritional	749:764	arg1	compounds					766:774	The anti-nutritional compounds	745:774	The anti-nutritional compounds	745:774	The anti-nutritional compounds were separated mostly to the fine protein-rich fraction.
25462921	0	25	from	Effect	0:5	arg1	properties					133:142	faba bean (Vicia faba L.) flour nutritional properties	89:142	faba bean (Vicia faba L.) flour nutritional properties	89:142	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	0	26	theme	faba	89:92	arg1	bean					94:97	faba bean	89:97	faba bean (Vicia faba L.) flour nutritional properties	89:142	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	0	26	theme	faba	89:92	arg1	L.					111:112	Vicia faba L.	100:112	Vicia faba L.	100:112	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	8	27	theme	protein	1259:1265	arg1	digestibility					1267:1279	the in vitro protein digestibility	1246:1279	the in vitro protein digestibility	1246:1279	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	0	28	theme	fermentation	33:44	arg1	Effect					0:5	Effect	0:5	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.	0:143	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	0	29	theme	air	10:12	arg1	classification					14:27	air classification	10:27	air classification	10:27	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	6	30	theme	vicine	869:874	arg1	contents					890:897	vicine and convicine contents	869:897	vicine and convicine contents	869:897	Fermentation caused the decrease of vicine and convicine contents by more than 91% and significantly reduced trypsin inhibitor activity and condensed tannins (by more than 40% in the protein-rich fraction).
25462921	1	31	theme	trypsin	285:291	arg1	factors					254:260	anti-nutritional factors	237:260	anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid)	237:346	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	31	theme	trypsin	285:291	arg1	activity					303:310	trypsin inhibitor activity	285:310	trypsin inhibitor activity	285:310	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	2	32	theme	amino	432:436	arg1	FAA					444:446	FAA	444:446	FAA	444:446	Free amino acid (FAA) profile analysis was also carried out.
25462921	2	32	theme	amino	432:436	arg1	acid					438:441	Free amino acid	427:441	Free amino acid (FAA) profile analysis	427:464	Free amino acid (FAA) profile analysis was also carried out.
25462921	1	33	dep	in	352:353	arg1	vitro					355:359	vitro	355:359	vitro	355:359	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	3	34	theme	different	591:599	arg1	compositions					610:621	different chemical compositions	591:621	different chemical compositions	591:621	Air classification allowed the separation of the flour into protein and starch rich fractions, showing different chemical compositions and microstructures.
25462921	7	35	theme	phytic	1109:1114	arg1	content					1121:1127	phytic acid content	1109:1127	phytic acid content	1109:1127	No significant (P>0.05) variation was observed for total phenols and phytic acid content.
25462921	3	36	theme	rich	567:570	arg1	fractions					572:580	protein and starch rich fractions	548:580	fractions	572:580	Air classification allowed the separation of the flour into protein and starch rich fractions, showing different chemical compositions and microstructures.
25462921	8	37	theme	γ-aminobutyric	1216:1229	arg1	acid					1231:1234	γ-aminobutyric acid	1216:1234	γ-aminobutyric acid	1216:1234	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	9	38	theme	air	1370:1372	arg1	classification					1374:1387	air classification	1370:1387	air classification	1370:1387	This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.
25462921	8	39	dep	in	1250:1251	arg1	vitro					1253:1257	vitro	1253:1257	vitro	1253:1257	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	7	40	theme	total	1091:1095	arg1	phenols					1097:1103	total phenols	1091:1103	total phenols	1091:1103	No significant (P>0.05) variation was observed for total phenols and phytic acid content.
25462921	1	41	theme	factors	254:260	arg1	reduction					224:232	the reduction	220:232	the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein	220:367	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	41	theme	factors	254:260	arg1	digestibility					380:392	starch digestibility	373:392	starch digestibility of faba bean flour	373:411	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	9	42	theme	faba	1444:1447	arg1	flour					1454:1458	faba bean flour	1444:1458	faba bean flour	1444:1458	This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.
25462921	6	43	theme	convicine	880:888	arg1	contents					890:897	vicine and convicine contents	869:897	vicine and convicine contents	869:897	Fermentation caused the decrease of vicine and convicine contents by more than 91% and significantly reduced trypsin inhibitor activity and condensed tannins (by more than 40% in the protein-rich fraction).
25462921	8	44	theme	amino	1200:1204	arg1	acids					1206:1210	the essential amino acids	1186:1210	the essential amino acids	1186:1210	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	1	45	theme	air	160:162	arg1	classification					164:177	air classification	160:177	air classification	160:177	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	6	46	theme	condensed	973:981	arg1	tannins					983:989	condensed tannins	973:989	condensed tannins (by more than 40% in the protein-rich fraction)	973:1037	Fermentation caused the decrease of vicine and convicine contents by more than 91% and significantly reduced trypsin inhibitor activity and condensed tannins (by more than 40% in the protein-rich fraction).
25462921	7	47	theme	significant	1043:1053	arg1	variation					1064:1072	No significant (P>0.05) variation	1040:1072	No significant (P>0.05) variation	1040:1072	No significant (P>0.05) variation was observed for total phenols and phytic acid content.
25462921	7	47	theme	significant	1043:1053	arg1	P>0.05					1056:1061	P>0.05	1056:1061	P>0.05	1056:1061	No significant (P>0.05) variation was observed for total phenols and phytic acid content.
25462921	9	48	theme	flour	1454:1458	arg1	functionality					1427:1439	nutritional functionality	1415:1439	nutritional functionality of faba bean flour which could be utilized in various food applications	1415:1511	This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.
25462921	8	49	theme	FAA	1167:1169	arg1	FAA					1167:1169	FAA	1167:1169	FAA	1167:1169	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	8	49	theme	FAA	1167:1169	arg1	amount					1157:1162	the amount	1153:1162	the amount	1153:1162	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	8	49	theme	FAA	1167:1169	arg1	acids					1206:1210	the essential amino acids	1186:1210	the essential amino acids	1186:1210	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	8	49	theme	FAA	1167:1169	arg1	acid					1231:1234	γ-aminobutyric acid	1216:1234	γ-aminobutyric acid	1216:1234	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	8	50	theme	acid	1231:1234	arg1	FAA					1167:1169	FAA	1167:1169	FAA	1167:1169	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	8	50	theme	acid	1231:1234	arg1	amount					1157:1162	the amount	1153:1162	the amount	1153:1162	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	8	50	theme	acid	1231:1234	arg1	acids					1206:1210	the essential amino acids	1186:1210	the essential amino acids	1186:1210	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	8	50	theme	acid	1231:1234	arg1	acid					1231:1234	γ-aminobutyric acid	1216:1234	γ-aminobutyric acid	1216:1234	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	0	51	theme	flour	115:119	arg1	properties					133:142	faba bean (Vicia faba L.) flour nutritional properties	89:142	faba bean (Vicia faba L.) flour nutritional properties	89:142	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	1	52	theme	protein	361:367	arg1	reduction					224:232	the reduction	220:232	the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein	220:367	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	52	theme	protein	361:367	arg1	digestibility					380:392	starch digestibility	373:392	starch digestibility of faba bean flour	373:411	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	3	53	theme	protein	548:554	arg1	fractions					572:580	protein and starch rich fractions	548:580	fractions	572:580	Air classification allowed the separation of the flour into protein and starch rich fractions, showing different chemical compositions and microstructures.
25462921	1	54	theme	classification	164:177	arg1	effects					149:155	The effects	145:155	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour	145:411	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	55	theme	acid	190:193	arg1	bacteria					195:202	lactic acid bacteria	183:202	lactic acid bacteria	183:202	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	6	56	theme	trypsin	942:948	arg1	activity					960:967	trypsin inhibitor activity	942:967	trypsin inhibitor activity	942:967	Fermentation caused the decrease of vicine and convicine contents by more than 91% and significantly reduced trypsin inhibitor activity and condensed tannins (by more than 40% in the protein-rich fraction).
25462921	1	57	theme	starch	373:378	arg1	digestibility					380:392	starch digestibility	373:392	starch digestibility of faba bean flour	373:411	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	3	58	theme	flour	537:541	arg1	separation					519:528	the separation	515:528	the separation of the flour into protein and starch rich fractions, showing different chemical compositions and microstructures	515:641	Air classification allowed the separation of the flour into protein and starch rich fractions, showing different chemical compositions and microstructures.
25462921	1	59	dep	factors	254:260	arg1	activity					303:310	trypsin inhibitor activity	285:310	trypsin inhibitor activity	285:310	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	59	dep	factors	254:260	arg1	acid					342:345	phytic acid	335:345	phytic acid	335:345	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	59	dep	factors	254:260	arg1	vicine					263:268	vicine	263:268	vicine	263:268	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	59	dep	factors	254:260	arg1	convicine					274:282	convicine	274:282	convicine	274:282	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	59	dep	factors	254:260	arg1	tannins					323:329	condensed tannins	313:329	condensed tannins	313:329	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	59	dep	factors	254:260	arg1	factors					254:260	anti-nutritional factors	237:260	anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid)	237:346	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	8	60	theme	in	1250:1251	arg1	digestibility					1267:1279	the in vitro protein digestibility	1246:1279	the in vitro protein digestibility	1246:1279	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	9	61	theme	various	1487:1493	arg1	applications					1500:1511	various food applications	1487:1511	various food applications	1487:1511	This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.
25462921	4	62	theme	bean	701:704	arg1	flour					706:710	faba bean flour	696:710	faba bean flour	696:710	Lactobacillus plantarum growth and acidification in faba bean flour and its fractions were assessed.
25462921	0	63	theme	VTT	73:75	arg1	E-133328					77:84	Lactobacillus plantarum VTT E-133328	49:84	Lactobacillus plantarum VTT E-133328	49:84	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	1	64	theme	bean	402:405	arg1	flour					407:411	faba bean flour	397:411	faba bean flour	397:411	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	3	65	theme	starch	560:565	arg1	fractions					572:580	protein and starch rich fractions	548:580	fractions	572:580	Air classification allowed the separation of the flour into protein and starch rich fractions, showing different chemical compositions and microstructures.
25462921	2	66	theme	profile	449:455	arg1	analysis					457:464	Free amino acid (FAA) profile analysis	427:464	Free amino acid (FAA) profile analysis	427:464	Free amino acid (FAA) profile analysis was also carried out.
25462921	4	67	theme	plantarum	658:666	arg1	growth					668:673	Lactobacillus plantarum growth	644:673	Lactobacillus plantarum growth	644:673	Lactobacillus plantarum growth and acidification in faba bean flour and its fractions were assessed.
25462921	0	68	theme	bean	94:97	arg1	properties					133:142	faba bean (Vicia faba L.) flour nutritional properties	89:142	faba bean (Vicia faba L.) flour nutritional properties	89:142	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	1	69	theme	phytic	335:340	arg1	factors					254:260	anti-nutritional factors	237:260	anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid)	237:346	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	69	theme	phytic	335:340	arg1	acid					342:345	phytic acid	335:345	phytic acid	335:345	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	9	70	used	utilized	1475:1482	arg2	functionality					1427:1439	nutritional functionality	1415:1439	nutritional functionality of faba bean flour which could be utilized in various food applications	1415:1511	This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.
25462921	6	71	from	%	1007:1007	arg1	fraction					1029:1036	the protein-rich fraction	1012:1036	the protein-rich fraction	1012:1036	Fermentation caused the decrease of vicine and convicine contents by more than 91% and significantly reduced trypsin inhibitor activity and condensed tannins (by more than 40% in the protein-rich fraction).
25462921	8	72	theme	hydrolysis	1311:1320	arg1	index					1322:1326	the hydrolysis index	1307:1326	the hydrolysis index	1307:1326	Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index.
25462921	0	73	theme	Vicia	100:104	arg1	bean					94:97	faba bean	89:97	faba bean (Vicia faba L.) flour nutritional properties	89:142	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	0	73	theme	Vicia	100:104	arg1	L.					111:112	Vicia faba L.	100:112	Vicia faba L.	100:112	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	1	74	theme	in	352:353	arg1	protein					361:367	in vitro protein	352:367	in vitro protein	352:367	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	0	75	theme	classification	14:27	arg1	Effect					0:5	Effect	0:5	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.	0:143	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	3	76	theme	chemical	601:608	arg1	compositions					610:621	different chemical compositions	591:621	different chemical compositions	591:621	Air classification allowed the separation of the flour into protein and starch rich fractions, showing different chemical compositions and microstructures.
25462921	7	77	theme	acid	1116:1119	arg1	content					1121:1127	phytic acid content	1109:1127	phytic acid content	1109:1127	No significant (P>0.05) variation was observed for total phenols and phytic acid content.
25462921	3	78	dep	fractions	572:580	arg1	showing					583:589	showing	583:589	showing different chemical compositions and microstructures	583:641	Air classification allowed the separation of the flour into protein and starch rich fractions, showing different chemical compositions and microstructures.
25462921	6	79	theme	protein-rich	1016:1027	arg1	fraction					1029:1036	the protein-rich fraction	1012:1036	the protein-rich fraction	1012:1036	Fermentation caused the decrease of vicine and convicine contents by more than 91% and significantly reduced trypsin inhibitor activity and condensed tannins (by more than 40% in the protein-rich fraction).
25462921	2	80	theme	acid	438:441	arg1	analysis					457:464	Free amino acid (FAA) profile analysis	427:464	Free amino acid (FAA) profile analysis	427:464	Free amino acid (FAA) profile analysis was also carried out.
25462921	1	81	dep	classification	164:177	arg1	fermentation					204:215	fermentation	204:215	fermentation	204:215	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	0	82	theme	Lactobacillus	49:61	arg1	E-133328					77:84	Lactobacillus plantarum VTT E-133328	49:84	Lactobacillus plantarum VTT E-133328	49:84	Effect of air classification and fermentation by Lactobacillus plantarum VTT E-133328 on faba bean (Vicia faba L.) flour nutritional properties.
25462921	1	83	theme	inhibitor	293:301	arg1	factors					254:260	anti-nutritional factors	237:260	anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid)	237:346	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	1	83	theme	inhibitor	293:301	arg1	activity					303:310	trypsin inhibitor activity	285:310	trypsin inhibitor activity	285:310	The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied.
25462921	2	84	theme	Free	427:430	arg1	FAA					444:446	FAA	444:446	FAA	444:446	Free amino acid (FAA) profile analysis was also carried out.
25462921	2	84	theme	Free	427:430	arg1	acid					438:441	Free amino acid	427:441	Free amino acid (FAA) profile analysis	427:464	Free amino acid (FAA) profile analysis was also carried out.
25462921	4	85	from	acidification	679:691	arg1	fractions					720:728	its fractions	716:728	its fractions	716:728	Lactobacillus plantarum growth and acidification in faba bean flour and its fractions were assessed.
25462921	4	85	from	acidification	679:691	arg1	flour					706:710	faba bean flour	696:710	faba bean flour	696:710	Lactobacillus plantarum growth and acidification in faba bean flour and its fractions were assessed.
25462921	9	86	theme	classification	1374:1387	arg1	combination					1355:1365	the combination	1351:1365	the combination of air classification and fermentation	1351:1404	This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.
27516256	1	0	theme	conduits	171:178	arg1	method					132:137	a fabrication method	118:137	a fabrication method of epineurium-mimicking tubular conduits based on electrodeposition from chitosan solution	118:228	In this investigation, we report on a fabrication method of epineurium-mimicking tubular conduits based on electrodeposition from chitosan solution.
27516256	3	1	theme	nerve	581:585	arg1	implants					587:594	peripheral nerve implants	570:594	peripheral nerve implants	570:594	In order to determine the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants, we perform chemical, physical, and biological studies.
27516256	3	2	theme	chemical	608:615	arg1	studies					643:649	chemical, physical, and biological studies	608:649	chemical, physical, and biological studies	608:649	In order to determine the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants, we perform chemical, physical, and biological studies.
27516256	4	3	theme	additives	722:730	arg1	crucial					748:754	crucial	748:754	crucial	748:754	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	4	3	theme	additives	722:730	arg1	weight					671:676	the molecular weight	657:676	the molecular weight of hyaluronic acid	657:695	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	4	3	theme	additives	722:730	arg1	concentration					705:717	the concentration	701:717	the concentration of additives	701:730	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	5	4	theme	application	978:988	arg1	potential					990:998	their application potential	972:998	their application potential on an animal model	972:1017	Because, the obtained structures show biocompatibility when contacting with a mouse hippocampal cell line (mHippoE-18), we further plan to test their application potential on an animal model.
27516256	2	5	with	pre-enrichment	235:248	arg1	acid					296:299	hyaluronic acid	285:299	hyaluronic acid	285:299	The pre-enrichment of electrodeposition solution with hyaluronic acid and/or collagen components results in structures which structural, morphological, and physicochemical properties can be controlled.
27516256	2	5	with	pre-enrichment	235:248	arg1	components					317:326	collagen components	308:326	collagen components	308:326	The pre-enrichment of electrodeposition solution with hyaluronic acid and/or collagen components results in structures which structural, morphological, and physicochemical properties can be controlled.
27516256	5	6	theme	cell	924:927	arg1	line					929:932	mouse hippocampal cell line	906:932	a mouse hippocampal cell line (mHippoE-18)	904:945	Because, the obtained structures show biocompatibility when contacting with a mouse hippocampal cell line (mHippoE-18), we further plan to test their application potential on an animal model.
27516256	5	6	theme	cell	924:927	arg1	mHippoE-18					935:944	mHippoE-18	935:944	mHippoE-18	935:944	Because, the obtained structures show biocompatibility when contacting with a mouse hippocampal cell line (mHippoE-18), we further plan to test their application potential on an animal model.
27516256	3	7	theme	chitosan	494:501	arg1	solution					503:510	the initial chitosan solution	482:510	the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants	482:594	In order to determine the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants, we perform chemical, physical, and biological studies.
27516256	3	8	theme	optimal	459:465	arg1	composition					467:477	the optimal composition	455:477	the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants	455:594	In order to determine the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants, we perform chemical, physical, and biological studies.
27516256	3	9	theme	solution	503:510	arg1	composition					467:477	the optimal composition	455:477	the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants	455:594	In order to determine the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants, we perform chemical, physical, and biological studies.
27516256	0	10	theme	chitosan	21:28	arg1	conduits					30:37	chitosan conduits	21:37	chitosan conduits	21:37	Epineurium-mimicking chitosan conduits for peripheral nervous tissue engineering.
27516256	2	11	theme	morphological	368:380	arg1	properties					403:412	structural, morphological, and physicochemical properties	356:412	structural, morphological, and physicochemical properties	356:412	The pre-enrichment of electrodeposition solution with hyaluronic acid and/or collagen components results in structures which structural, morphological, and physicochemical properties can be controlled.
27516256	3	12	theme	biological	632:641	arg1	studies					643:649	chemical, physical, and biological studies	608:649	chemical, physical, and biological studies	608:649	In order to determine the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants, we perform chemical, physical, and biological studies.
27516256	1	13	theme	chitosan	212:219	arg1	solution					221:228	chitosan solution	212:228	chitosan solution	212:228	In this investigation, we report on a fabrication method of epineurium-mimicking tubular conduits based on electrodeposition from chitosan solution.
27516256	2	14	theme	structural	356:365	arg1	properties					403:412	structural, morphological, and physicochemical properties	356:412	structural, morphological, and physicochemical properties	356:412	The pre-enrichment of electrodeposition solution with hyaluronic acid and/or collagen components results in structures which structural, morphological, and physicochemical properties can be controlled.
27516256	5	15	theme	animal	1006:1011	arg1	model					1013:1017	an animal model	1003:1017	an animal model	1003:1017	Because, the obtained structures show biocompatibility when contacting with a mouse hippocampal cell line (mHippoE-18), we further plan to test their application potential on an animal model.
27516256	4	16	theme	biological	792:801	arg1	performance					803:813	the final mechanical as well as biological performance	760:813	the final mechanical as well as biological performance of conduits	760:825	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	4	17	theme	acid	692:695	arg1	crucial					748:754	crucial	748:754	crucial	748:754	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	4	17	theme	acid	692:695	arg1	weight					671:676	the molecular weight	657:676	the molecular weight of hyaluronic acid	657:695	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	4	17	theme	acid	692:695	arg1	concentration					705:717	the concentration	701:717	the concentration of additives	701:730	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	0	18	theme	nervous	54:60	arg1	engineering					69:79	peripheral nervous tissue engineering	43:79	peripheral nervous tissue engineering	43:79	Epineurium-mimicking chitosan conduits for peripheral nervous tissue engineering.
27516256	2	19	theme	physicochemical	387:401	arg1	properties					403:412	structural, morphological, and physicochemical properties	356:412	structural, morphological, and physicochemical properties	356:412	The pre-enrichment of electrodeposition solution with hyaluronic acid and/or collagen components results in structures which structural, morphological, and physicochemical properties can be controlled.
27516256	3	20	theme	initial	486:492	arg1	solution					503:510	the initial chitosan solution	482:510	the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants	482:594	In order to determine the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants, we perform chemical, physical, and biological studies.
27516256	5	21	theme	mouse	906:910	arg1	line					929:932	mouse hippocampal cell line	906:932	a mouse hippocampal cell line (mHippoE-18)	904:945	Because, the obtained structures show biocompatibility when contacting with a mouse hippocampal cell line (mHippoE-18), we further plan to test their application potential on an animal model.
27516256	5	21	theme	mouse	906:910	arg1	mHippoE-18					935:944	mHippoE-18	935:944	mHippoE-18	935:944	Because, the obtained structures show biocompatibility when contacting with a mouse hippocampal cell line (mHippoE-18), we further plan to test their application potential on an animal model.
27516256	0	22	theme	peripheral	43:52	arg1	engineering					69:79	peripheral nervous tissue engineering	43:79	peripheral nervous tissue engineering	43:79	Epineurium-mimicking chitosan conduits for peripheral nervous tissue engineering.
27516256	2	23	theme	solution	271:278	arg1	pre-enrichment					235:248	The pre-enrichment	231:248	The pre-enrichment of electrodeposition solution with hyaluronic acid and/or collagen components	231:326	The pre-enrichment of electrodeposition solution with hyaluronic acid and/or collagen components results in structures which structural, morphological, and physicochemical properties can be controlled.
27516256	5	24	theme	hippocampal	912:922	arg1	line					929:932	mouse hippocampal cell line	906:932	a mouse hippocampal cell line (mHippoE-18)	904:945	Because, the obtained structures show biocompatibility when contacting with a mouse hippocampal cell line (mHippoE-18), we further plan to test their application potential on an animal model.
27516256	5	24	theme	hippocampal	912:922	arg1	mHippoE-18					935:944	mHippoE-18	935:944	mHippoE-18	935:944	Because, the obtained structures show biocompatibility when contacting with a mouse hippocampal cell line (mHippoE-18), we further plan to test their application potential on an animal model.
27516256	5	25	theme	obtained	841:848	arg1	structures					850:859	the obtained structures	837:859	the obtained structures	837:859	Because, the obtained structures show biocompatibility when contacting with a mouse hippocampal cell line (mHippoE-18), we further plan to test their application potential on an animal model.
27516256	3	26	theme	peripheral	570:579	arg1	implants					587:594	peripheral nerve implants	570:594	peripheral nerve implants	570:594	In order to determine the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants, we perform chemical, physical, and biological studies.
27516256	2	27	theme	electrodeposition	253:269	arg1	solution					271:278	electrodeposition solution	253:278	electrodeposition solution	253:278	The pre-enrichment of electrodeposition solution with hyaluronic acid and/or collagen components results in structures which structural, morphological, and physicochemical properties can be controlled.
27516256	4	28	theme	final	764:768	arg1	performance					803:813	the final mechanical as well as biological performance	760:813	the final mechanical as well as biological performance of conduits	760:825	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	0	29	theme	tissue	62:67	arg1	engineering					69:79	peripheral nervous tissue engineering	43:79	peripheral nervous tissue engineering	43:79	Epineurium-mimicking chitosan conduits for peripheral nervous tissue engineering.
27516256	1	30	from	solution	221:228	arg1	electrodeposition					189:205	electrodeposition	189:205	electrodeposition from chitosan solution	189:228	In this investigation, we report on a fabrication method of epineurium-mimicking tubular conduits based on electrodeposition from chitosan solution.
27516256	1	31	theme	fabrication	120:130	arg1	method					132:137	a fabrication method	118:137	a fabrication method of epineurium-mimicking tubular conduits based on electrodeposition from chitosan solution	118:228	In this investigation, we report on a fabrication method of epineurium-mimicking tubular conduits based on electrodeposition from chitosan solution.
27516256	2	32	theme	collagen	308:315	arg1	components					317:326	collagen components	308:326	collagen components	308:326	The pre-enrichment of electrodeposition solution with hyaluronic acid and/or collagen components results in structures which structural, morphological, and physicochemical properties can be controlled.
27516256	3	33	theme	physical	618:625	arg1	studies					643:649	chemical, physical, and biological studies	608:649	chemical, physical, and biological studies	608:649	In order to determine the optimal composition of the initial chitosan solution resulting in conduits meeting the requirements imposed on peripheral nerve implants, we perform chemical, physical, and biological studies.
27516256	4	34	theme	conduits	818:825	arg1	performance					803:813	the final mechanical as well as biological performance	760:813	the final mechanical as well as biological performance of conduits	760:825	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	4	35	theme	mechanical	770:779	arg1	performance					803:813	the final mechanical as well as biological performance	760:813	the final mechanical as well as biological performance of conduits	760:825	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	4	36	theme	molecular	661:669	arg1	crucial					748:754	crucial	748:754	crucial	748:754	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	4	36	theme	molecular	661:669	arg1	weight					671:676	the molecular weight	657:676	the molecular weight of hyaluronic acid	657:695	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	1	37	theme	epineurium-mimicking	142:161	arg1	conduits					171:178	epineurium-mimicking tubular conduits	142:178	epineurium-mimicking tubular conduits based on electrodeposition from chitosan solution	142:228	In this investigation, we report on a fabrication method of epineurium-mimicking tubular conduits based on electrodeposition from chitosan solution.
27516256	5	38	from	potential	990:998	arg1	model					1013:1017	an animal model	1003:1017	an animal model	1003:1017	Because, the obtained structures show biocompatibility when contacting with a mouse hippocampal cell line (mHippoE-18), we further plan to test their application potential on an animal model.
27516256	4	39	theme	hyaluronic	681:690	arg1	acid					692:695	hyaluronic acid	681:695	hyaluronic acid	681:695	Both the molecular weight of hyaluronic acid and the concentration of additives are found to be crucial for the final mechanical as well as biological performance of conduits.
27516256	2	40	theme	hyaluronic	285:294	arg1	acid					296:299	hyaluronic acid	285:299	hyaluronic acid	285:299	The pre-enrichment of electrodeposition solution with hyaluronic acid and/or collagen components results in structures which structural, morphological, and physicochemical properties can be controlled.
27516256	1	41	theme	tubular	163:169	arg1	conduits					171:178	epineurium-mimicking tubular conduits	142:178	epineurium-mimicking tubular conduits based on electrodeposition from chitosan solution	142:228	In this investigation, we report on a fabrication method of epineurium-mimicking tubular conduits based on electrodeposition from chitosan solution.
25817663	0	0	theme	morphological	65:77	arg1	properties					79:88	the thermal, mechanical and morphological properties	37:88	the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films	37:134	Influence of alkali metal cations on the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films.
25817663	9	1	theme	thermal	1078:1084	arg1	stabilities					1086:1096	the thermal stabilities	1074:1096	the thermal stabilities	1074:1096	In addition, the thermal stabilities significantly varied when alkali metal cations varied from Li(+) to Cs(+).
25817663	2	2	theme	ion-exchanged	463:475	arg1	clay					481:484	ion-exchanged REC clay	463:484	ion-exchanged REC clay	463:484	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	11	3	theme	packaging	1378:1386	arg1	applications					1388:1399	packaging applications	1378:1399	packaging applications	1378:1399	After evaluating these properties of BNCFs, we came to conclusion that these bio-nano composites can be used for packaging applications.
25817663	10	4	theme	tensile	1234:1240	arg1	GPa					1259:1261	up to 45 GPa	1250:1261	up to 45 GPa	1250:1261	The BNCFs featured high tensile strengths (up to 84 MPa) and tensile moduli (up to 45 GPa).
25817663	10	4	theme	tensile	1234:1240	arg1	moduli					1242:1247	tensile moduli	1234:1247	tensile moduli (up to 45 GPa)	1234:1262	The BNCFs featured high tensile strengths (up to 84 MPa) and tensile moduli (up to 45 GPa).
25817663	11	5	theme	bio-nano	1342:1349	arg1	composites					1351:1360	these bio-nano composites	1336:1360	these bio-nano composites	1336:1360	After evaluating these properties of BNCFs, we came to conclusion that these bio-nano composites can be used for packaging applications.
25817663	3	6	theme	BNCFs	524:528	arg1	morphology					510:519	morphology	510:519	morphology	510:519	The microstructure and morphology of BNCFs were investigated with XRD, TEM, SEM and AFM.
25817663	3	6	theme	BNCFs	524:528	arg1	microstructure					491:504	microstructure	491:504	microstructure	491:504	The microstructure and morphology of BNCFs were investigated with XRD, TEM, SEM and AFM.
25817663	1	7	theme	morphological	303:315	arg1	properties					317:326	the thermal, mechanical and morphological properties	275:326	the thermal, mechanical and morphological properties	275:326	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	1	8	theme	work	160:163	arg1	theme					146:150	The main theme	137:150	The main theme of this work	137:163	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	2	9	with	reaction	437:444	arg1	clay					481:484	ion-exchanged REC clay	463:484	ion-exchanged REC clay	463:484	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	2	10	theme	REC-CS	374:379	arg1	BNCFs					407:411	BNCFs	407:411	BNCFs	407:411	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	2	10	theme	REC-CS	374:379	arg1	films					400:404	rectorite/chitosan (REC-CS) bio-nanocomposite films	354:404	rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs)	354:412	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	2	11	theme	films	400:404	arg1	set					347:349	a set	345:349	a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs)	345:412	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	2	11	theme	films	400:404	arg1	BNCFs					407:411	BNCFs	407:411	BNCFs	407:411	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	2	11	theme	films	400:404	arg1	films					400:404	rectorite/chitosan (REC-CS) bio-nanocomposite films	354:404	rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs)	354:412	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	0	12	from	Influence	0:8	arg1	properties					79:88	the thermal, mechanical and morphological properties	37:88	the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films	37:134	Influence of alkali metal cations on the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films.
25817663	2	13	theme	chitosan	449:456	arg1	reaction					437:444	facile reaction	430:444	facile reaction of chitosan with ion-exchanged REC clay	430:484	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	4	14	theme	BNCFs	610:614	arg1	properties					596:605	Thermal and tensile properties	576:605	Thermal and tensile properties of BNCFs	576:614	Thermal and tensile properties of BNCFs were also investigated.
25817663	8	15	dep	clay	1034:1037	arg1	polymer					1052:1058	polymer	1052:1058	polymer	1052:1058	FTIR confirmed strong hydrogen bonds between clay and chitosan polymer.
25817663	2	16	theme	bio-nanocomposite	382:398	arg1	BNCFs					407:411	BNCFs	407:411	BNCFs	407:411	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	2	16	theme	bio-nanocomposite	382:398	arg1	films					400:404	rectorite/chitosan (REC-CS) bio-nanocomposite films	354:404	rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs)	354:412	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	6	17	theme	clay	735:738	arg1	sheets					740:745	Some intercalated clay sheets	717:745	Some intercalated clay sheets	717:745	Some intercalated clay sheets, together with nano-sized clay tactoids were obtained in LiREC/CS, NaREC/CS and KREC/CS of the BNCFs.
25817663	10	18	theme	high	1192:1195	arg1	strengths					1205:1213	high tensile strengths	1192:1213	high tensile strengths (up to 84 MPa)	1192:1228	The BNCFs featured high tensile strengths (up to 84 MPa) and tensile moduli (up to 45 GPa).
25817663	10	18	theme	high	1192:1195	arg1	MPa					1225:1227	up to 84 MPa	1216:1227	up to 84 MPa	1216:1227	The BNCFs featured high tensile strengths (up to 84 MPa) and tensile moduli (up to 45 GPa).
25817663	10	19	dep	84	1222:1223	arg1	to					1219:1220	to	1219:1220	to	1219:1220	The BNCFs featured high tensile strengths (up to 84 MPa) and tensile moduli (up to 45 GPa).
25817663	6	20	dep	together	748:755	arg1	with					757:760	with	757:760	with	757:760	Some intercalated clay sheets, together with nano-sized clay tactoids were obtained in LiREC/CS, NaREC/CS and KREC/CS of the BNCFs.
25817663	0	21	theme	bio-nanocomposite	112:128	arg1	films					130:134	rectorite/chitosan bio-nanocomposite films	93:134	rectorite/chitosan bio-nanocomposite films	93:134	Influence of alkali metal cations on the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films.
25817663	6	22	theme	intercalated	722:733	arg1	sheets					740:745	Some intercalated clay sheets	717:745	Some intercalated clay sheets	717:745	Some intercalated clay sheets, together with nano-sized clay tactoids were obtained in LiREC/CS, NaREC/CS and KREC/CS of the BNCFs.
25817663	7	23	attach	attaches	944:951	arg1	platelets					978:986	the clay platelets	969:986	(and covers) the clay platelets	956:986	From fractured surface study, via SEM, it was observed that the dispersion of chitosan polymer attaches to (and covers) the clay platelets.
25817663	7	23	attach	attaches	944:951	arg2	dispersion					913:922	the dispersion	909:922	the dispersion of chitosan polymer	909:942	From fractured surface study, via SEM, it was observed that the dispersion of chitosan polymer attaches to (and covers) the clay platelets.
25817663	0	24	theme	rectorite/chitosan	93:110	arg1	films					130:134	rectorite/chitosan bio-nanocomposite films	93:134	rectorite/chitosan bio-nanocomposite films	93:134	Influence of alkali metal cations on the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films.
25817663	9	25	theme	alkali	1124:1129	arg1	cations					1137:1143	alkali metal cations	1124:1143	alkali metal cations	1124:1143	In addition, the thermal stabilities significantly varied when alkali metal cations varied from Li(+) to Cs(+).
25817663	7	26	theme	clay	973:976	arg1	platelets					978:986	the clay platelets	969:986	(and covers) the clay platelets	956:986	From fractured surface study, via SEM, it was observed that the dispersion of chitosan polymer attaches to (and covers) the clay platelets.
25817663	9	27	theme	metal	1131:1135	arg1	cations					1137:1143	alkali metal cations	1124:1143	alkali metal cations	1124:1143	In addition, the thermal stabilities significantly varied when alkali metal cations varied from Li(+) to Cs(+).
25817663	5	28	theme	mixed	699:703	arg1	morphology					705:714	a mixed morphology	697:714	a mixed morphology	697:714	As revealed from TEM and XRD results, the BNCFs featured a mixed morphology.
25817663	5	29	theme	TEM	657:659	arg1	results					669:675	TEM and XRD results	657:675	TEM and XRD results	657:675	As revealed from TEM and XRD results, the BNCFs featured a mixed morphology.
25817663	2	30	theme	REC	477:479	arg1	clay					481:484	ion-exchanged REC clay	463:484	ion-exchanged REC clay	463:484	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	1	31	theme	thermal	279:285	arg1	properties					317:326	the thermal, mechanical and morphological properties	275:326	the thermal, mechanical and morphological properties	275:326	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	7	32	theme	polymer	936:942	arg1	dispersion					913:922	the dispersion	909:922	the dispersion of chitosan polymer	909:942	From fractured surface study, via SEM, it was observed that the dispersion of chitosan polymer attaches to (and covers) the clay platelets.
25817663	0	33	theme	metal	20:24	arg1	cations					26:32	alkali metal cations	13:32	alkali metal cations	13:32	Influence of alkali metal cations on the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films.
25817663	4	34	theme	Thermal	576:582	arg1	properties					596:605	Thermal and tensile properties	576:605	Thermal and tensile properties of BNCFs	576:614	Thermal and tensile properties of BNCFs were also investigated.
25817663	7	35	theme	chitosan	927:934	arg1	polymer					936:942	chitosan polymer	927:942	chitosan polymer	927:942	From fractured surface study, via SEM, it was observed that the dispersion of chitosan polymer attaches to (and covers) the clay platelets.
25817663	0	36	theme	alkali	13:18	arg1	cations					26:32	alkali metal cations	13:32	alkali metal cations	13:32	Influence of alkali metal cations on the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films.
25817663	0	37	theme	films	130:134	arg1	properties					79:88	the thermal, mechanical and morphological properties	37:88	the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films	37:134	Influence of alkali metal cations on the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films.
25817663	4	38	theme	tensile	588:594	arg1	properties					596:605	Thermal and tensile properties	576:605	Thermal and tensile properties of BNCFs	576:614	Thermal and tensile properties of BNCFs were also investigated.
25817663	8	39	theme	hydrogen	1011:1018	arg1	bonds					1020:1024	strong hydrogen bonds	1004:1024	strong hydrogen bonds between clay and chitosan polymer	1004:1058	FTIR confirmed strong hydrogen bonds between clay and chitosan polymer.
25817663	1	40	theme	ion-exchangeable	194:209	arg1	cations					224:230	ion-exchangeable alkali metal cations	194:230	ion-exchangeable alkali metal cations	194:230	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	0	41	theme	cations	26:32	arg1	Influence					0:8	Influence	0:8	Influence of alkali metal cations on the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films.	0:135	Influence of alkali metal cations on the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films.
25817663	8	42	theme	strong	1004:1009	arg1	bonds					1020:1024	strong hydrogen bonds	1004:1024	strong hydrogen bonds between clay and chitosan polymer	1004:1058	FTIR confirmed strong hydrogen bonds between clay and chitosan polymer.
25817663	1	43	theme	alkali	211:216	arg1	cations					224:230	ion-exchangeable alkali metal cations	194:230	ion-exchangeable alkali metal cations	194:230	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	0	44	theme	thermal	41:47	arg1	properties					79:88	the thermal, mechanical and morphological properties	37:88	the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films	37:134	Influence of alkali metal cations on the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films.
25817663	7	45	dep	platelets	978:986	arg1	and					957:959	and	957:959	and	957:959	From fractured surface study, via SEM, it was observed that the dispersion of chitosan polymer attaches to (and covers) the clay platelets.
25817663	1	46	dep	cations	224:230	arg1	Cs					266:267	Cs	266:267	Cs	266:267	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	1	46	dep	cations	224:230	arg1	K					256:256	K	256:256	K	256:256	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	1	46	dep	cations	224:230	arg1	Na					249:250	Na	249:250	Na	249:250	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	1	46	dep	cations	224:230	arg1	Li					242:243	Li	242:243	Li	242:243	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	7	47	dep	and	957:959	arg1	covers					961:966	covers	961:966	covers	961:966	From fractured surface study, via SEM, it was observed that the dispersion of chitosan polymer attaches to (and covers) the clay platelets.
25817663	6	48	theme	clay	773:776	arg1	tactoids					778:785	nano-sized clay tactoids	762:785	nano-sized clay tactoids	762:785	Some intercalated clay sheets, together with nano-sized clay tactoids were obtained in LiREC/CS, NaREC/CS and KREC/CS of the BNCFs.
25817663	2	49	theme	facile	430:435	arg1	reaction					437:444	facile reaction	430:444	facile reaction of chitosan with ion-exchanged REC clay	430:484	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	1	50	dep	Li	242:243	arg1	+					245:245	+	245:245	+	245:245	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	1	50	dep	Li	242:243	arg1	such					233:236	such	233:236	such	233:236	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	1	51	from	influence	181:189	arg1	properties					317:326	the thermal, mechanical and morphological properties	275:326	the thermal, mechanical and morphological properties	275:326	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	10	52	theme	tensile	1197:1203	arg1	strengths					1205:1213	high tensile strengths	1192:1213	high tensile strengths (up to 84 MPa)	1192:1228	The BNCFs featured high tensile strengths (up to 84 MPa) and tensile moduli (up to 45 GPa).
25817663	10	52	theme	tensile	1197:1203	arg1	MPa					1225:1227	up to 84 MPa	1216:1227	up to 84 MPa	1216:1227	The BNCFs featured high tensile strengths (up to 84 MPa) and tensile moduli (up to 45 GPa).
25817663	6	53	theme	nano-sized	762:771	arg1	tactoids					778:785	nano-sized clay tactoids	762:785	nano-sized clay tactoids	762:785	Some intercalated clay sheets, together with nano-sized clay tactoids were obtained in LiREC/CS, NaREC/CS and KREC/CS of the BNCFs.
25817663	7	54	theme	fractured	854:862	arg1	study					872:876	fractured surface study	854:876	fractured surface study	854:876	From fractured surface study, via SEM, it was observed that the dispersion of chitosan polymer attaches to (and covers) the clay platelets.
25817663	10	55	dep	45	1256:1257	arg1	to					1253:1254	to	1253:1254	to	1253:1254	The BNCFs featured high tensile strengths (up to 84 MPa) and tensile moduli (up to 45 GPa).
25817663	11	56	theme	BNCFs	1302:1306	arg1	properties					1288:1297	these properties	1282:1297	these properties of BNCFs	1282:1306	After evaluating these properties of BNCFs, we came to conclusion that these bio-nano composites can be used for packaging applications.
25817663	1	57	theme	metal	218:222	arg1	cations					224:230	ion-exchangeable alkali metal cations	194:230	ion-exchangeable alkali metal cations	194:230	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	7	58	theme	surface	864:870	arg1	study					872:876	fractured surface study	854:876	fractured surface study	854:876	From fractured surface study, via SEM, it was observed that the dispersion of chitosan polymer attaches to (and covers) the clay platelets.
25817663	1	59	theme	mechanical	288:297	arg1	properties					317:326	the thermal, mechanical and morphological properties	275:326	the thermal, mechanical and morphological properties	275:326	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	1	60	theme	main	141:144	arg1	theme					146:150	The main theme	137:150	The main theme of this work	137:163	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	1	61	theme	cations	224:230	arg1	influence					181:189	the influence	177:189	the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties	177:326	The main theme of this work is to study the influence of ion-exchangeable alkali metal cations, such as: Li(+), Na(+), K(+), and Cs(+) on the thermal, mechanical and morphological properties.
25817663	0	62	theme	mechanical	50:59	arg1	properties					79:88	the thermal, mechanical and morphological properties	37:88	the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films	37:134	Influence of alkali metal cations on the thermal, mechanical and morphological properties of rectorite/chitosan bio-nanocomposite films.
25817663	11	63	used	used	1369:1372	arg2	composites					1351:1360	these bio-nano composites	1336:1360	these bio-nano composites	1336:1360	After evaluating these properties of BNCFs, we came to conclusion that these bio-nano composites can be used for packaging applications.
25817663	6	64	theme	BNCFs	842:846	arg1	KREC/CS					827:833	KREC/CS	827:833	KREC/CS of the BNCFs	827:846	Some intercalated clay sheets, together with nano-sized clay tactoids were obtained in LiREC/CS, NaREC/CS and KREC/CS of the BNCFs.
25817663	2	65	theme	rectorite/chitosan	354:371	arg1	BNCFs					407:411	BNCFs	407:411	BNCFs	407:411	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	2	65	theme	rectorite/chitosan	354:371	arg1	films					400:404	rectorite/chitosan (REC-CS) bio-nanocomposite films	354:404	rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs)	354:412	In this regard, a set of rectorite/chitosan (REC-CS) bio-nanocomposite films (BNCFs) was prepared by facile reaction of chitosan with ion-exchanged REC clay.
25817663	5	66	theme	XRD	665:667	arg1	results					669:675	TEM and XRD results	657:675	TEM and XRD results	657:675	As revealed from TEM and XRD results, the BNCFs featured a mixed morphology.
25817663	3	67	dep	microstructure	491:504	arg1	The					487:489	The	487:489	The	487:489	The microstructure and morphology of BNCFs were investigated with XRD, TEM, SEM and AFM.
27500601	1	0	theme	homogeneous	491:501	arg1	glycoproteins					518:530	homogeneous M6P-containing glycoproteins	491:530	homogeneous M6P-containing glycoproteins	491:530	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	8	1	theme	enzymes	1855:1861	arg1	delivery					1843:1850	delivery	1843:1850	delivery	1843:1850	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	8	1	theme	enzymes	1855:1861	arg1	uptake					1832:1837	uptake	1832:1837	uptake	1832:1837	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	1	2	theme	hydrolases	309:318	arg1	targeting					278:286	M6P-receptor-mediated targeting	256:286	M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes	256:342	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	7	3	theme	M6P	1579:1581	arg1	moieties					1583:1590	the M6P moieties	1575:1590	the M6P moieties	1575:1590	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	8	4	theme	enzymes	1809:1815	arg1	remodeling					1783:1792	glycosylation remodeling	1769:1792	glycosylation remodeling of recombinant enzymes	1769:1815	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	6	5	theme	ligands	1428:1434	arg1	proximity					1391:1399	a close proximity	1383:1399	a close proximity of two M6P-oligosaccharide ligands	1383:1434	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	6	5	theme	ligands	1428:1434	arg1	critical					1439:1446	critical	1439:1446	critical	1439:1446	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	7	6	theme	oligosaccharide	1606:1620	arg1	context					1622:1628	the right oligosaccharide context	1596:1628	the right oligosaccharide context	1596:1628	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	7	6	theme	oligosaccharide	1606:1620	arg1	critical					1638:1645	critical	1638:1645	critical	1638:1645	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	4	7	theme	bivalent	997:1004	arg1	M6P-glycopeptides					1006:1022	bivalent M6P-glycopeptides	997:1022	bivalent M6P-glycopeptides	997:1022	Simultaneous attachment of two M6P-oligosaccahrides to a cyclic polypeptide was also accomplished to yield bivalent M6P-glycopeptides.
27500601	5	8	from	α-1,6-branch	1263:1274	arg1	dispensable					1279:1289	dispensable	1279:1289	dispensable	1279:1289	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	5	8	from	α-1,6-branch	1263:1274	arg1	presence					1231:1238	the presence	1227:1238	the presence of a M6P moiety at the α-1,6-branch	1227:1274	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	7	9	with	binding	1665:1671	arg1	receptor					1692:1699	the major M6P receptor	1678:1699	the major M6P receptor	1678:1699	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	5	10	theme	plasmon	1033:1039	arg1	resonance					1041:1049	Surface plasmon resonance	1025:1049	Surface plasmon resonance binding studies	1025:1065	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	6	11	theme	binding	1307:1313	arg1	study					1315:1319	a binding study	1305:1319	a binding study with the bivalent cyclic and linear polypeptides	1305:1368	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	4	12	attach	attachment	903:912	arg2	M6P-oligosaccahrides					921:940	two M6P-oligosaccahrides	917:940	two M6P-oligosaccahrides	917:940	Simultaneous attachment of two M6P-oligosaccahrides to a cyclic polypeptide was also accomplished to yield bivalent M6P-glycopeptides.
27500601	4	12	attach	attachment	903:912	arg1	polypeptide					954:964	a cyclic polypeptide	945:964	a cyclic polypeptide	945:964	Simultaneous attachment of two M6P-oligosaccahrides to a cyclic polypeptide was also accomplished to yield bivalent M6P-glycopeptides.
27500601	5	13	theme	binding	1051:1057	arg1	studies					1059:1065	Surface plasmon resonance binding studies	1025:1065	Surface plasmon resonance binding studies	1025:1065	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	6	14	with	study	1315:1319	arg1	polypeptides					1357:1368	the bivalent cyclic and linear polypeptides	1326:1368	the bivalent cyclic and linear polypeptides	1326:1368	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	3	15	theme	M6P-containing	860:873	arg1	glycoproteins					875:887	homogeneous M6P-containing glycoproteins	848:887	homogeneous M6P-containing glycoproteins	848:887	The method includes the chemical synthesis of glycan oxazolines with varied number and location of the M6P moieties and their transfer to the GlcNAc-protein by an endoglycosynthase to provide homogeneous M6P-containing glycoproteins.
27500601	1	16	from	Golgi	325:329	arg1	hydrolases					309:318	newly synthesized hydrolases	291:318	newly synthesized hydrolases from Golgi	291:329	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	1	16	from	Golgi	325:329	arg1	targeting					278:286	M6P-receptor-mediated targeting	256:286	M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes	256:342	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	1	17	theme	important	234:242	arg1	oligosaccharides					213:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides	176:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides	176:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	1	17	theme	important	234:242	arg1	signals					244:250	important signals	234:250	important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes	234:342	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	6	18	theme	close	1385:1389	arg1	proximity					1391:1399	a close proximity	1383:1399	a close proximity of two M6P-oligosaccharide ligands	1383:1434	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	6	18	theme	close	1385:1389	arg1	critical					1439:1446	critical	1439:1446	critical	1439:1446	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	0	19	theme	M6P	150:152	arg1	Recognition					163:173	M6P Receptor Recognition	150:173	M6P Receptor Recognition	150:173	Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition.
27500601	7	20	theme	present	1518:1524	arg1	study					1526:1530	the present study	1514:1530	the present study	1514:1530	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	3	21	theme	glycan	702:707	arg1	oxazolines					709:718	glycan oxazolines	702:718	glycan oxazolines	702:718	The method includes the chemical synthesis of glycan oxazolines with varied number and location of the M6P moieties and their transfer to the GlcNAc-protein by an endoglycosynthase to provide homogeneous M6P-containing glycoproteins.
27500601	8	22	theme	storage	1937:1943	arg1	diseases					1945:1952	lysosomal storage diseases	1927:1952	lysosomal storage diseases	1927:1952	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	5	23	theme	M6P	1088:1090	arg1	moiety					1092:1097	a single M6P moiety	1079:1097	a single M6P moiety located at the low α-1,3-branch of the oligomannose context	1079:1157	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	5	23	theme	M6P	1088:1090	arg1	sufficient					1162:1171	sufficient	1162:1171	sufficient	1162:1171	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	1	24	from	targeting	278:286	arg1	Golgi					325:329	Golgi	325:329	Golgi	325:329	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	6	25	theme	bivalent	1330:1337	arg1	polypeptides					1357:1368	the bivalent cyclic and linear polypeptides	1326:1368	the bivalent cyclic and linear polypeptides	1326:1368	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	5	26	theme	located	1099:1105	arg1	moiety					1092:1097	a single M6P moiety	1079:1097	a single M6P moiety located at the low α-1,3-branch of the oligomannose context	1079:1157	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	5	26	theme	located	1099:1105	arg1	sufficient					1162:1171	sufficient	1162:1171	sufficient	1162:1171	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	2	27	theme	homogeneous	580:590	arg1	phosphoglycoproteins					592:611	homogeneous phosphoglycoproteins	580:611	homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans	580:653	We describe here a chemoenzymatic synthesis of homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans.
27500601	7	28	dep	location	1551:1558	arg1	the					1547:1549	the	1547:1549	the	1547:1549	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	1	29	gly	glycoproteins	518:530	arg1	glycoproteins					518:530	homogeneous M6P-containing glycoproteins	491:530	homogeneous M6P-containing glycoproteins	491:530	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	0	30	theme	-Containing	74:84	arg1	Ligands					99:105	Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands	49:105	Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands	49:105	Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition.
27500601	1	31	theme	M6P	392:394	arg1	recognition					412:422	the M6P ligand-receptor recognition	388:422	the M6P ligand-receptor recognition	388:422	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	2	32	theme	M6P-containing	630:643	arg1	N-glycans					645:653	natural M6P-containing N-glycans	622:653	natural M6P-containing N-glycans	622:653	We describe here a chemoenzymatic synthesis of homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans.
27500601	0	33	theme	Ligands	99:105	arg1	Synthesis					15:23	Chemoenzymatic Synthesis	0:23	Chemoenzymatic Synthesis	0:23	Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition.
27500601	0	33	theme	Ligands	99:105	arg1	Binding					38:44	Receptor Binding	29:44	Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands	29:105	Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition.
27500601	5	34	theme	moiety	1249:1254	arg1	dispensable					1279:1289	dispensable	1279:1289	dispensable	1279:1289	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	5	34	theme	moiety	1249:1254	arg1	presence					1231:1238	the presence	1227:1238	the presence of a M6P moiety at the α-1,6-branch	1227:1274	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	4	35	theme	M6P-oligosaccahrides	921:940	arg1	attachment					903:912	Simultaneous attachment	890:912	Simultaneous attachment of two M6P-oligosaccahrides to a cyclic polypeptide	890:964	Simultaneous attachment of two M6P-oligosaccahrides to a cyclic polypeptide was also accomplished to yield bivalent M6P-glycopeptides.
27500601	0	36	theme	Unusual	114:120	arg1	Requirements					133:144	Unusual Structural Requirements	114:144	Unusual Structural Requirements for M6P Receptor Recognition	114:173	Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition.
27500601	5	37	theme	context	1151:1157	arg1	α-1,3-branch					1118:1129	the low α-1,3-branch	1110:1129	the low α-1,3-branch of the oligomannose context	1110:1157	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	5	38	attach	presence	1231:1238	arg2	moiety					1249:1254	a M6P moiety	1243:1254	a M6P moiety	1243:1254	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	5	38	attach	presence	1231:1238	arg1	α-1,6-branch					1263:1274	the α-1,6-branch	1259:1274	the α-1,6-branch	1259:1274	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	0	39	theme	Chemoenzymatic	0:13	arg1	Synthesis					15:23	Chemoenzymatic Synthesis	0:23	Chemoenzymatic Synthesis	0:23	Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition.
27500601	4	40	theme	Simultaneous	890:901	arg1	attachment					903:912	Simultaneous attachment	890:912	Simultaneous attachment of two M6P-oligosaccahrides to a cyclic polypeptide	890:964	Simultaneous attachment of two M6P-oligosaccahrides to a cyclic polypeptide was also accomplished to yield bivalent M6P-glycopeptides.
27500601	7	41	theme	M6P	1688:1690	arg1	receptor					1692:1699	the major M6P receptor	1678:1699	the major M6P receptor	1678:1699	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	8	42	theme	chemoenzymatic	1706:1719	arg1	method					1721:1726	The chemoenzymatic method	1702:1726	The chemoenzymatic method described here	1702:1741	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	3	43	theme	moieties	763:770	arg1	number					732:737	number	732:737	number	732:737	The method includes the chemical synthesis of glycan oxazolines with varied number and location of the M6P moieties and their transfer to the GlcNAc-protein by an endoglycosynthase to provide homogeneous M6P-containing glycoproteins.
27500601	3	43	theme	moieties	763:770	arg1	transfer					782:789	their transfer	776:789	their transfer to the GlcNAc-protein	776:811	The method includes the chemical synthesis of glycan oxazolines with varied number and location of the M6P moieties and their transfer to the GlcNAc-protein by an endoglycosynthase to provide homogeneous M6P-containing glycoproteins.
27500601	3	43	theme	moieties	763:770	arg1	location					743:750	location	743:750	location	743:750	The method includes the chemical synthesis of glycan oxazolines with varied number and location of the M6P moieties and their transfer to the GlcNAc-protein by an endoglycosynthase to provide homogeneous M6P-containing glycoproteins.
27500601	5	44	theme	Surface	1025:1031	arg1	resonance					1041:1049	Surface plasmon resonance	1025:1049	Surface plasmon resonance binding studies	1025:1065	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	8	45	from	lysosomes	1866:1874	arg1	therapy					1898:1904	enzyme replacement therapy	1879:1904	enzyme replacement therapy for the treatment of lysosomal storage diseases	1879:1952	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	1	46	theme	structural	361:370	arg1	requirement					372:382	the precise structural requirement	349:382	the precise structural requirement for the M6P ligand-receptor recognition	349:422	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	5	47	theme	receptor	1204:1211	arg1	CI-MPR					1213:1218	receptor CI-MPR	1204:1218	receptor CI-MPR	1204:1218	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	5	48	from	α-1,3-branch	1118:1129	arg1	located					1099:1105	located	1099:1105	located	1099:1105	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	1	49	theme	M6P-receptor-mediated	256:276	arg1	targeting					278:286	M6P-receptor-mediated targeting	256:286	M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes	256:342	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	8	50	theme	enzyme	1879:1884	arg1	therapy					1898:1904	enzyme replacement therapy	1879:1904	enzyme replacement therapy for the treatment of lysosomal storage diseases	1879:1952	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	2	51	contain	carrying	613:620	arg2	N-glycans					645:653	natural M6P-containing N-glycans	622:653	natural M6P-containing N-glycans	622:653	We describe here a chemoenzymatic synthesis of homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans.
27500601	2	51	contain	carrying	613:620	arg1	phosphoglycoproteins					592:611	homogeneous phosphoglycoproteins	580:611	homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans	580:653	We describe here a chemoenzymatic synthesis of homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans.
27500601	1	52	theme	synthesized	297:307	arg1	hydrolases					309:318	newly synthesized hydrolases	291:318	newly synthesized hydrolases from Golgi	291:329	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	1	53	theme	M6P-containing	503:516	arg1	glycoproteins					518:530	homogeneous M6P-containing glycoproteins	491:530	homogeneous M6P-containing glycoproteins	491:530	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	7	54	theme	right	1600:1604	arg1	context					1622:1628	the right oligosaccharide context	1596:1628	the right oligosaccharide context	1596:1628	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	7	54	theme	right	1600:1604	arg1	critical					1638:1645	critical	1638:1645	critical	1638:1645	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	7	55	theme	moieties	1583:1590	arg1	valency					1564:1570	valency	1564:1570	valency	1564:1570	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	7	55	theme	moieties	1583:1590	arg1	location					1551:1558	location	1551:1558	location	1551:1558	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	7	55	theme	moieties	1583:1590	arg1	context					1622:1628	the right oligosaccharide context	1596:1628	the right oligosaccharide context	1596:1628	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	7	55	theme	moieties	1583:1590	arg1	critical					1638:1645	critical	1638:1645	critical	1638:1645	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	8	56	theme	recombinant	1797:1807	arg1	enzymes					1809:1815	recombinant enzymes	1797:1815	recombinant enzymes	1797:1815	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	3	57	theme	homogeneous	848:858	arg1	glycoproteins					875:887	homogeneous M6P-containing glycoproteins	848:887	homogeneous M6P-containing glycoproteins	848:887	The method includes the chemical synthesis of glycan oxazolines with varied number and location of the M6P moieties and their transfer to the GlcNAc-protein by an endoglycosynthase to provide homogeneous M6P-containing glycoproteins.
27500601	6	58	theme	M6P-oligosaccharide	1408:1426	arg1	ligands					1428:1434	two M6P-oligosaccharide ligands	1404:1434	two M6P-oligosaccharide ligands	1404:1434	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	1	59	theme	-terminated	201:211	arg1	oligosaccharides					213:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides	176:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides	176:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	1	59	theme	-terminated	201:211	arg1	signals					244:250	important signals	234:250	important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes	234:342	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	1	60	theme	M6P	197:199	arg1	oligosaccharides					213:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides	176:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides	176:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	1	60	theme	M6P	197:199	arg1	signals					244:250	important signals	234:250	important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes	234:342	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	3	61	theme	chemical	680:687	arg1	synthesis					689:697	the chemical synthesis	676:697	the chemical synthesis of glycan oxazolines	676:718	The method includes the chemical synthesis of glycan oxazolines with varied number and location of the M6P moieties and their transfer to the GlcNAc-protein by an endoglycosynthase to provide homogeneous M6P-containing glycoproteins.
27500601	5	62	theme	resonance	1041:1049	arg1	studies					1059:1065	Surface plasmon resonance binding studies	1025:1065	Surface plasmon resonance binding studies	1025:1065	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	8	63	theme	replacement	1886:1896	arg1	therapy					1898:1904	enzyme replacement therapy	1879:1904	enzyme replacement therapy for the treatment of lysosomal storage diseases	1879:1952	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	0	64	theme	Receptor	154:161	arg1	Recognition					163:173	M6P Receptor Recognition	150:173	M6P Receptor Recognition	150:173	Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition.
27500601	3	65	theme	oxazolines	709:718	arg1	synthesis					689:697	the chemical synthesis	676:697	the chemical synthesis of glycan oxazolines	676:718	The method includes the chemical synthesis of glycan oxazolines with varied number and location of the M6P moieties and their transfer to the GlcNAc-protein by an endoglycosynthase to provide homogeneous M6P-containing glycoproteins.
27500601	4	66	gly	M6P-glycopeptides	1006:1022	arg2	M6P-glycopeptides					1006:1022	bivalent M6P-glycopeptides	997:1022	bivalent M6P-glycopeptides	997:1022	Simultaneous attachment of two M6P-oligosaccahrides to a cyclic polypeptide was also accomplished to yield bivalent M6P-glycopeptides.
27500601	5	67	theme	M6P	1245:1247	arg1	moiety					1249:1254	a M6P moiety	1243:1254	a M6P moiety	1243:1254	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	5	68	theme	single	1081:1086	arg1	moiety					1092:1097	a single M6P moiety	1079:1097	a single M6P moiety located at the low α-1,3-branch of the oligomannose context	1079:1157	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	5	68	theme	single	1081:1086	arg1	sufficient					1162:1171	sufficient	1162:1171	sufficient	1162:1171	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	6	69	theme	high	1459:1462	arg1	affinity					1464:1471	high affinity	1459:1471	high affinity for the CI-MPR receptor	1459:1495	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	8	70	theme	lysosomal	1927:1935	arg1	diseases					1945:1952	lysosomal storage diseases	1927:1952	lysosomal storage diseases	1927:1952	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	6	71	theme	linear	1350:1355	arg1	polypeptides					1357:1368	the bivalent cyclic and linear polypeptides	1326:1368	the bivalent cyclic and linear polypeptides	1326:1368	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	8	72	dep	uptake	1832:1837	arg1	the					1828:1830	the	1828:1830	the	1828:1830	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	8	73	theme	diseases	1945:1952	arg1	treatment					1914:1922	the treatment	1910:1922	the treatment of lysosomal storage diseases	1910:1952	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	6	74	theme	cyclic	1339:1344	arg1	polypeptides					1357:1368	the bivalent cyclic and linear polypeptides	1326:1368	the bivalent cyclic and linear polypeptides	1326:1368	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	0	75	theme	Glycoprotein	86:97	arg1	Ligands					99:105	Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands	49:105	Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands	49:105	Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition.
27500601	1	76	theme	Mannose-6-phosphate	176:194	arg1	oligosaccharides					213:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides	176:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides	176:228	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	1	76	theme	Mannose-6-phosphate	176:194	arg1	signals					244:250	important signals	234:250	important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes	234:342	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	2	77	theme	chemoenzymatic	552:565	arg1	synthesis					567:575	a chemoenzymatic synthesis	550:575	a chemoenzymatic synthesis of homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans	550:653	We describe here a chemoenzymatic synthesis of homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans.
27500601	1	78	theme	ligand-receptor	396:410	arg1	recognition					412:422	the M6P ligand-receptor recognition	388:422	the M6P ligand-receptor recognition	388:422	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	2	79	theme	natural	622:628	arg1	N-glycans					645:653	natural M6P-containing N-glycans	622:653	natural M6P-containing N-glycans	622:653	We describe here a chemoenzymatic synthesis of homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans.
27500601	0	80	theme	Structural	122:131	arg1	Requirements					133:144	Unusual Structural Requirements	114:144	Unusual Structural Requirements for M6P Receptor Recognition	114:173	Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition.
27500601	2	81	gly	phosphoglycoproteins	592:611	arg1	phosphoglycoproteins					592:611	homogeneous phosphoglycoproteins	580:611	homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans	580:653	We describe here a chemoenzymatic synthesis of homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans.
27500601	2	82	theme	phosphoglycoproteins	592:611	arg1	synthesis					567:575	a chemoenzymatic synthesis	550:575	a chemoenzymatic synthesis of homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans	550:653	We describe here a chemoenzymatic synthesis of homogeneous phosphoglycoproteins carrying natural M6P-containing N-glycans.
27500601	3	83	gly	glycoproteins	875:887	arg1	glycoproteins					875:887	homogeneous M6P-containing glycoproteins	848:887	homogeneous M6P-containing glycoproteins	848:887	The method includes the chemical synthesis of glycan oxazolines with varied number and location of the M6P moieties and their transfer to the GlcNAc-protein by an endoglycosynthase to provide homogeneous M6P-containing glycoproteins.
27500601	8	84	theme	new	1754:1756	arg1	avenue					1758:1763	a new avenue	1752:1763	a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases	1752:1952	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	6	85	theme	CI-MPR	1481:1486	arg1	receptor					1488:1495	the CI-MPR receptor	1477:1495	the CI-MPR receptor	1477:1495	In addition, a binding study with the bivalent cyclic and linear polypeptides reveals that a close proximity of two M6P-oligosaccharide ligands is critical to achieve high affinity for the CI-MPR receptor.
27500601	0	86	theme	Receptor	29:36	arg1	Binding					38:44	Receptor Binding	29:44	Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands	29:105	Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition.
27500601	5	87	theme	low	1114:1116	arg1	α-1,3-branch					1118:1129	the low α-1,3-branch	1110:1129	the low α-1,3-branch of the oligomannose context	1110:1157	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	5	88	theme	oligomannose	1138:1149	arg1	context					1151:1157	the oligomannose context	1134:1157	the oligomannose context	1134:1157	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	3	89	theme	M6P	759:761	arg1	moieties					763:770	the M6P moieties	755:770	the M6P moieties	755:770	The method includes the chemical synthesis of glycan oxazolines with varied number and location of the M6P moieties and their transfer to the GlcNAc-protein by an endoglycosynthase to provide homogeneous M6P-containing glycoproteins.
27500601	5	90	theme	high-affinity	1179:1191	arg1	binding					1193:1199	a high-affinity binding	1177:1199	a high-affinity binding to receptor CI-MPR	1177:1218	Surface plasmon resonance binding studies reveal that a single M6P moiety located at the low α-1,3-branch of the oligomannose context is sufficient for a high-affinity binding to receptor CI-MPR, while the presence of a M6P moiety at the α-1,6-branch is dispensable.
27500601	1	91	theme	precise	353:359	arg1	requirement					372:382	the precise structural requirement	349:382	the precise structural requirement for the M6P ligand-receptor recognition	349:422	Mannose-6-phosphate (M6P)-terminated oligosaccharides are important signals for M6P-receptor-mediated targeting of newly synthesized hydrolases from Golgi to lysosomes, but the precise structural requirement for the M6P ligand-receptor recognition has not been fully understood due to the difficulties in obtaining homogeneous M6P-containing glycoproteins.
27500601	7	92	theme	major	1682:1686	arg1	receptor					1692:1699	the major M6P receptor	1678:1699	the major M6P receptor	1678:1699	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	7	93	theme	high-affinity	1651:1663	arg1	binding					1665:1671	high-affinity binding	1651:1671	high-affinity binding with the major M6P receptor	1651:1699	Taken together, the present study indicates that the location and valency of the M6P moieties and the right oligosaccharide context are all critical for high-affinity binding with the major M6P receptor.
27500601	8	94	theme	glycosylation	1769:1781	arg1	remodeling					1783:1792	glycosylation remodeling	1769:1792	glycosylation remodeling of recombinant enzymes	1769:1815	The chemoenzymatic method described here provides a new avenue for glycosylation remodeling of recombinant enzymes to enhance the uptake and delivery of enzymes to lysosomes in enzyme replacement therapy for the treatment of lysosomal storage diseases.
27500601	4	95	theme	cyclic	947:952	arg1	polypeptide					954:964	a cyclic polypeptide	945:964	a cyclic polypeptide	945:964	Simultaneous attachment of two M6P-oligosaccahrides to a cyclic polypeptide was also accomplished to yield bivalent M6P-glycopeptides.
26627590	0	0	theme	critical	85:92	arg1	parameters					94:103	the critical parameters	81:103	the critical parameters	81:103	Inverse gas chromatography for natural fibre characterisation: Identification of the critical parameters to determine the Brunauer-Emmett-Teller specific surface area.
26627590	4	1	theme	0.51m	688:692	arg1	g					696:696	0.51m(2)g(-1)	688:700	0.51m(2)g(-1)	688:700	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	4	2	theme	0.54m	784:788	arg1	former					738:743	former	738:743	former	738:743	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	4	2	theme	0.54m	784:788	arg1	g					792:792	0.54m(2)g(-1)	784:796	0.54m(2)g(-1)	784:796	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	4	2	theme	0.54m	784:788	arg1	similar					751:757	similar	751:757	similar	751:757	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	1	3	theme	gas	176:178	arg1	technique					219:227	an alternative technique	204:227	an alternative technique to determine the specific surface area of natural fibres	204:284	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
26627590	1	3	theme	gas	176:178	arg1	IGC					196:198	IGC	196:198	IGC	196:198	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
26627590	1	3	theme	gas	176:178	arg1	chromatography					180:193	Inverse gas chromatography	168:193	Inverse gas chromatography (IGC)	168:199	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
26627590	8	4	theme	optical	1339:1345	arg1	microscopy					1347:1356	optical microscopy	1339:1356	optical microscopy	1339:1356	The higher surface area of the flax sample, and its higher variability, was correlated with a higher surface roughness observed under optical microscopy.
26627590	3	5	theme	surface	553:559	arg1	®					604:604	®	604:604	®	604:604	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	3	5	theme	surface	553:559	arg1	area					561:564	Brunauer-Emmett-Teller (BET) specific surface area	515:564	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	7	6	theme	comparison	1107:1116	arg1	effects					1147:1153	any misleading data comparison due to molecular orientation effects	1087:1153	any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions	1087:1202	The findings showed the choice of solvent was important for all specimens to avoid any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions.
26627590	7	7	theme	solvent	1038:1044	arg1	choice					1028:1033	the choice	1024:1033	the choice of solvent	1024:1044	The findings showed the choice of solvent was important for all specimens to avoid any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions.
26627590	9	8	theme	chromatography	1371:1384	arg1	column					1386:1391	the chromatography column	1367:1391	Packing the chromatography column with long or chopped fibres	1359:1419	Packing the chromatography column with long or chopped fibres produced results that were statistically insignificant.
26627590	7	9	dep	showed	1017:1022	arg1	important					1050:1058	important	1050:1058	important	1050:1058	The findings showed the choice of solvent was important for all specimens to avoid any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions.
26627590	9	10	theme	chopped	1406:1412	arg1	fibres					1414:1419	long or chopped fibres	1398:1419	long or chopped fibres	1398:1419	Packing the chromatography column with long or chopped fibres produced results that were statistically insignificant.
26627590	3	11	theme	flax	581:584	arg1	samples					570:576	samples	570:576	samples of flax, kenaf and BioMid	570:602	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	6	12	theme	surface	983:989	arg1	area					991:994	representative BET surface area	964:994	representative BET surface area values	964:1001	Repeatability was excellent so that two runs sufficed to obtain representative BET surface area values.
26627590	1	13	theme	natural	271:277	arg1	fibres					279:284	natural fibres	271:284	natural fibres	271:284	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
26627590	0	14	theme	parameters	94:103	arg1	Identification					63:76	Identification	63:76	Identification of the critical parameters	63:103	Inverse gas chromatography for natural fibre characterisation: Identification of the critical parameters to determine the Brunauer-Emmett-Teller specific surface area.
26627590	4	15	theme	BET	639:641	arg1	area					651:654	The BET surface area	635:654	The BET surface area of kenaf and flax	635:672	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	0	16	dep	determine	108:116	arg1	chromatography					12:25	Inverse gas chromatography	0:25	Inverse gas chromatography for natural fibre characterisation	0:60	Inverse gas chromatography for natural fibre characterisation: Identification of the critical parameters to determine the Brunauer-Emmett-Teller specific surface area.
26627590	1	17	theme	fibres	279:284	arg1	area					263:266	the specific surface area	242:266	the specific surface area of natural fibres	242:284	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
26627590	6	18	theme	BET	979:981	arg1	area					991:994	representative BET surface area	964:994	representative BET surface area values	964:1001	Repeatability was excellent so that two runs sufficed to obtain representative BET surface area values.
26627590	3	19	theme	measured	506:513	arg1	fibres					617:622	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	2	20	theme	Natural	287:293	arg1	fibres					295:300	Natural fibres	287:300	Natural fibres	287:300	Natural fibres have a complex surface chemistry and unique microstructure that challenge the current capabilities to perform surface characterisation.
26627590	7	21	theme	adsorbent-adsorbate	1171:1189	arg1	interactions					1191:1202	the adsorbent-adsorbate interactions	1167:1202	the adsorbent-adsorbate interactions	1167:1202	The findings showed the choice of solvent was important for all specimens to avoid any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions.
26627590	5	22	theme	BET	851:853	arg1	equation					855:862	the BET equation	847:862	the BET equation	847:862	The data was calculated under conditions where the BET equation showed good linearity (R(2)⩾0.995).
26627590	9	23	with	column	1386:1391	arg1	fibres					1414:1419	long or chopped fibres	1398:1419	long or chopped fibres	1398:1419	Packing the chromatography column with long or chopped fibres produced results that were statistically insignificant.
26627590	2	24	theme	surface	412:418	arg1	characterisation					420:435	surface characterisation	412:435	surface characterisation	412:435	Natural fibres have a complex surface chemistry and unique microstructure that challenge the current capabilities to perform surface characterisation.
26627590	0	25	theme	gas	8:10	arg1	chromatography					12:25	Inverse gas chromatography	0:25	Inverse gas chromatography for natural fibre characterisation	0:60	Inverse gas chromatography for natural fibre characterisation: Identification of the critical parameters to determine the Brunauer-Emmett-Teller specific surface area.
26627590	4	26	dep	similar	751:757	arg1	former					738:743	former	738:743	former	738:743	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	4	26	dep	similar	751:757	arg1	g					792:792	0.54m(2)g(-1)	784:796	0.54m(2)g(-1)	784:796	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	4	26	dep	similar	751:757	arg1	similar					751:757	similar	751:757	similar	751:757	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	4	27	theme	kenaf	659:663	arg1	area					651:654	The BET surface area	635:654	The BET surface area of kenaf and flax	635:672	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	3	28	theme	BioMid	597:602	arg1	samples					570:576	samples	570:576	samples of flax, kenaf and BioMid	570:602	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	4	29	dep	g	714:714	arg1	2					712:712	2	712:712	2	712:712	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	0	30	theme	Inverse	0:6	arg1	chromatography					12:25	Inverse gas chromatography	0:25	Inverse gas chromatography for natural fibre characterisation	0:60	Inverse gas chromatography for natural fibre characterisation: Identification of the critical parameters to determine the Brunauer-Emmett-Teller specific surface area.
26627590	4	31	theme	cellulose	766:774	arg1	fibres					776:781	the cellulose fibres	762:781	the cellulose fibres	762:781	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	2	32	theme	surface	317:323	arg1	chemistry					325:333	a complex surface chemistry	307:333	a complex surface chemistry	307:333	Natural fibres have a complex surface chemistry and unique microstructure that challenge the current capabilities to perform surface characterisation.
26627590	4	33	theme	flax	669:672	arg1	area					651:654	The BET surface area	635:654	The BET surface area of kenaf and flax	635:672	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	0	34	theme	specific	145:152	arg1	area					162:165	the Brunauer-Emmett-Teller specific surface area	118:165	the Brunauer-Emmett-Teller specific surface area	118:165	Inverse gas chromatography for natural fibre characterisation: Identification of the critical parameters to determine the Brunauer-Emmett-Teller specific surface area.
26627590	2	35	theme	complex	309:315	arg1	chemistry					325:333	a complex surface chemistry	307:333	a complex surface chemistry	307:333	Natural fibres have a complex surface chemistry and unique microstructure that challenge the current capabilities to perform surface characterisation.
26627590	4	36	theme	surface	643:649	arg1	area					651:654	The BET surface area	635:654	The BET surface area of kenaf and flax	635:672	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	8	37	theme	surface	1216:1222	arg1	area					1224:1227	The higher surface area	1205:1227	The higher surface area of the flax sample	1205:1246	The higher surface area of the flax sample, and its higher variability, was correlated with a higher surface roughness observed under optical microscopy.
26627590	4	38	dep	g	792:792	arg1	2					790:790	2	790:790	2	790:790	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	4	39	theme	1.35m	706:710	arg1	g					714:714	1.35m(2)g(-1)	706:718	1.35m(2)g(-1)	706:718	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	1	40	theme	alternative	207:217	arg1	technique					219:227	an alternative technique	204:227	an alternative technique to determine the specific surface area of natural fibres	204:284	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
26627590	1	40	theme	alternative	207:217	arg1	chromatography					180:193	Inverse gas chromatography	168:193	Inverse gas chromatography (IGC)	168:199	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
26627590	0	41	theme	fibre	39:43	arg1	characterisation					45:60	natural fibre characterisation	31:60	natural fibre characterisation	31:60	Inverse gas chromatography for natural fibre characterisation: Identification of the critical parameters to determine the Brunauer-Emmett-Teller specific surface area.
26627590	2	42	contain	have	302:305	arg1	fibres					295:300	Natural fibres	287:300	Natural fibres	287:300	Natural fibres have a complex surface chemistry and unique microstructure that challenge the current capabilities to perform surface characterisation.
26627590	2	42	contain	have	302:305	arg2	chemistry					325:333	a complex surface chemistry	307:333	a complex surface chemistry	307:333	Natural fibres have a complex surface chemistry and unique microstructure that challenge the current capabilities to perform surface characterisation.
26627590	2	42	contain	have	302:305	arg2	microstructure					346:359	unique microstructure	339:359	unique microstructure	339:359	Natural fibres have a complex surface chemistry and unique microstructure that challenge the current capabilities to perform surface characterisation.
26627590	4	43	dep	g	696:696	arg1	2					694:694	2	694:694	2	694:694	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	7	44	theme	molecular	1125:1133	arg1	orientation					1135:1145	molecular orientation	1125:1145	molecular orientation	1125:1145	The findings showed the choice of solvent was important for all specimens to avoid any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions.
26627590	3	45	theme	cellulose	607:615	arg1	fibres					617:622	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	3	46	theme	parameters	488:497	arg1	influence					466:474	the influence	462:474	the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	462:622	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	0	47	theme	natural	31:37	arg1	characterisation					45:60	natural fibre characterisation	31:60	natural fibre characterisation	31:60	Inverse gas chromatography for natural fibre characterisation: Identification of the critical parameters to determine the Brunauer-Emmett-Teller specific surface area.
26627590	0	48	theme	surface	154:160	arg1	area					162:165	the Brunauer-Emmett-Teller specific surface area	118:165	the Brunauer-Emmett-Teller specific surface area	118:165	Inverse gas chromatography for natural fibre characterisation: Identification of the critical parameters to determine the Brunauer-Emmett-Teller specific surface area.
26627590	8	49	theme	higher	1299:1304	arg1	roughness					1314:1322	a higher surface roughness	1297:1322	a higher surface roughness observed under optical microscopy	1297:1356	The higher surface area of the flax sample, and its higher variability, was correlated with a higher surface roughness observed under optical microscopy.
26627590	8	50	theme	sample	1241:1246	arg1	variability					1264:1274	its higher variability	1253:1274	its higher variability	1253:1274	The higher surface area of the flax sample, and its higher variability, was correlated with a higher surface roughness observed under optical microscopy.
26627590	8	50	theme	sample	1241:1246	arg1	area					1224:1227	The higher surface area	1205:1227	The higher surface area of the flax sample	1205:1246	The higher surface area of the flax sample, and its higher variability, was correlated with a higher surface roughness observed under optical microscopy.
26627590	3	51	theme	specific	544:551	arg1	®					604:604	®	604:604	®	604:604	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	3	51	theme	specific	544:551	arg1	area					561:564	Brunauer-Emmett-Teller (BET) specific surface area	515:564	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	3	52	theme	BET	539:541	arg1	®					604:604	®	604:604	®	604:604	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	3	52	theme	BET	539:541	arg1	area					561:564	Brunauer-Emmett-Teller (BET) specific surface area	515:564	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	9	53	theme	Packing	1359:1365	arg1	column					1386:1391	the chromatography column	1367:1391	Packing the chromatography column with long or chopped fibres	1359:1419	Packing the chromatography column with long or chopped fibres produced results that were statistically insignificant.
26627590	9	54	theme	long	1398:1401	arg1	fibres					1414:1419	long or chopped fibres	1398:1419	long or chopped fibres	1398:1419	Packing the chromatography column with long or chopped fibres produced results that were statistically insignificant.
26627590	6	55	theme	area	991:994	arg1	values					996:1001	representative BET surface area values	964:1001	representative BET surface area values	964:1001	Repeatability was excellent so that two runs sufficed to obtain representative BET surface area values.
26627590	7	56	theme	due	1118:1120	arg1	effects					1147:1153	any misleading data comparison due to molecular orientation effects	1087:1153	any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions	1087:1202	The findings showed the choice of solvent was important for all specimens to avoid any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions.
26627590	6	57	theme	representative	964:977	arg1	area					991:994	representative BET surface area	964:994	representative BET surface area values	964:1001	Repeatability was excellent so that two runs sufficed to obtain representative BET surface area values.
26627590	7	58	theme	misleading	1091:1100	arg1	effects					1147:1153	any misleading data comparison due to molecular orientation effects	1087:1153	any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions	1087:1202	The findings showed the choice of solvent was important for all specimens to avoid any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions.
26627590	8	59	theme	surface	1306:1312	arg1	roughness					1314:1322	a higher surface roughness	1297:1322	a higher surface roughness observed under optical microscopy	1297:1356	The higher surface area of the flax sample, and its higher variability, was correlated with a higher surface roughness observed under optical microscopy.
26627590	4	60	dep	differed	674:681	arg1	former					738:743	former	738:743	former	738:743	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	4	60	dep	differed	674:681	arg1	g					792:792	0.54m(2)g(-1)	784:796	0.54m(2)g(-1)	784:796	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	4	60	dep	differed	674:681	arg1	similar					751:757	similar	751:757	similar	751:757	The BET surface area of kenaf and flax differed with 0.51m(2)g(-1) and 1.35m(2)g(-1) respectively, the former being similar to the cellulose fibres (0.54m(2)g(-1)).
26627590	2	61	theme	unique	339:344	arg1	microstructure					346:359	unique microstructure	339:359	unique microstructure	339:359	Natural fibres have a complex surface chemistry and unique microstructure that challenge the current capabilities to perform surface characterisation.
26627590	3	62	theme	kenaf	587:591	arg1	samples					570:576	samples	570:576	samples of flax, kenaf and BioMid	570:602	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	8	63	theme	higher	1209:1214	arg1	area					1224:1227	The higher surface area	1205:1227	The higher surface area of the flax sample	1205:1246	The higher surface area of the flax sample, and its higher variability, was correlated with a higher surface roughness observed under optical microscopy.
26627590	8	64	theme	higher	1257:1262	arg1	variability					1264:1274	its higher variability	1253:1274	its higher variability	1253:1274	The higher surface area of the flax sample, and its higher variability, was correlated with a higher surface roughness observed under optical microscopy.
26627590	2	65	theme	current	380:386	arg1	capabilities					388:399	the current capabilities	376:399	the current capabilities	376:399	Natural fibres have a complex surface chemistry and unique microstructure that challenge the current capabilities to perform surface characterisation.
26627590	3	66	theme	Brunauer-Emmett-Teller	515:536	arg1	®					604:604	®	604:604	®	604:604	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	3	66	theme	Brunauer-Emmett-Teller	515:536	arg1	area					561:564	Brunauer-Emmett-Teller (BET) specific surface area	515:564	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	8	67	theme	flax	1236:1239	arg1	sample					1241:1246	the flax sample	1232:1246	the flax sample	1232:1246	The higher surface area of the flax sample, and its higher variability, was correlated with a higher surface roughness observed under optical microscopy.
26627590	5	68	theme	good	871:874	arg1	R					887:887	R(2)⩾0.995	887:896	R(2)⩾0.995	887:896	The data was calculated under conditions where the BET equation showed good linearity (R(2)⩾0.995).
26627590	5	68	theme	good	871:874	arg1	linearity					876:884	good linearity	871:884	good linearity (R(2)⩾0.995)	871:897	The data was calculated under conditions where the BET equation showed good linearity (R(2)⩾0.995).
26627590	3	69	from	influence	466:474	arg1	fibres					617:622	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	3	70	theme	area	561:564	arg1	fibres					617:622	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres	502:622	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	3	71	theme	multiple	479:486	arg1	parameters					488:497	multiple parameters	479:497	multiple parameters	479:497	This study investigated the influence of multiple parameters on the measured Brunauer-Emmett-Teller (BET) specific surface area for samples of flax, kenaf and BioMid(®) cellulose fibres using IGC.
26627590	1	72	theme	specific	246:253	arg1	area					263:266	the specific surface area	242:266	the specific surface area of natural fibres	242:284	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
26627590	7	73	theme	data	1102:1105	arg1	effects					1147:1153	any misleading data comparison due to molecular orientation effects	1087:1153	any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions	1087:1202	The findings showed the choice of solvent was important for all specimens to avoid any misleading data comparison due to molecular orientation effects that impact the adsorbent-adsorbate interactions.
26627590	1	74	theme	Inverse	168:174	arg1	technique					219:227	an alternative technique	204:227	an alternative technique to determine the specific surface area of natural fibres	204:284	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
26627590	1	74	theme	Inverse	168:174	arg1	IGC					196:198	IGC	196:198	IGC	196:198	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
26627590	1	74	theme	Inverse	168:174	arg1	chromatography					180:193	Inverse gas chromatography	168:193	Inverse gas chromatography (IGC)	168:199	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
26627590	1	75	theme	surface	255:261	arg1	area					263:266	the specific surface area	242:266	the specific surface area of natural fibres	242:284	Inverse gas chromatography (IGC) is an alternative technique to determine the specific surface area of natural fibres.
28841463	7	0	theme	cells	1235:1239	arg1	differentiation					1193:1207	osteochondrogenic differentiation	1175:1207	osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis	1175:1300	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	4	1	theme	following	718:726	arg1	grafts					737:742	the following vascular grafts	714:742	the following vascular grafts	714:742	Rat aortas were decellularized to create the following vascular grafts: 1) ECM hybridized with POCC (Poly-ECM), 2) Poly-ECM subsequently functionalized with heparin (Poly-ECM-Hep), and 3) non-modified vascular ECM.
28841463	6	2	theme	intramural	1066:1075	arg1	infiltration					1082:1093	minimal intramural cell infiltration	1058:1093	minimal intramural cell infiltration	1058:1093	All grafts displayed antioxidant activity, were patent, and exhibited minimal intramural cell infiltration with varying degrees of calcification.
28841463	7	3	theme	smooth	1221:1226	arg1	damage					1275:1280	oxidized DNA damage	1262:1280	oxidized DNA damage	1262:1280	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	3	theme	smooth	1221:1226	arg1	peroxidation					1248:1259	lipid peroxidation	1242:1259	lipid peroxidation	1242:1259	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	3	theme	smooth	1221:1226	arg1	cells					1235:1239	vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis	1212:1300	cells	1235:1239	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	3	theme	smooth	1221:1226	arg1	apoptosis					1292:1300	cell apoptosis	1287:1300	cell apoptosis	1287:1300	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	3	4	dep	poly	584:587	arg1	8-octamethylene-citrate-co-cysteine					592:626	8-octamethylene-citrate-co-cysteine	592:626	8-octamethylene-citrate-co-cysteine	592:626	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	3	4	dep	poly	584:587	arg1	1					589:589	1	589:589	1	589:589	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	4	5	theme	non-modified	861:872	arg1	ECM					883:885	3) non-modified vascular ECM	858:885	3) non-modified vascular ECM	858:885	Rat aortas were decellularized to create the following vascular grafts: 1) ECM hybridized with POCC (Poly-ECM), 2) Poly-ECM subsequently functionalized with heparin (Poly-ECM-Hep), and 3) non-modified vascular ECM.
28841463	2	6	theme	unpredictable	292:304	arg1	properties					317:326	thrombogenic and unpredictable remodeling properties	275:326	thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification	275:372	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	2	6	theme	unpredictable	292:304	arg1	calcification					360:372	calcification	360:372	calcification	360:372	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	2	6	theme	unpredictable	292:304	arg1	hyperplasia					344:354	intimal hyperplasia	336:354	intimal hyperplasia	336:354	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	5	7	theme	abdominal	941:949	arg1	aorta					951:955	the abdominal aorta	937:955	the abdominal aorta of adult rats at three months	937:985	Grafts were evaluated as interposition grafts in the abdominal aorta of adult rats at three months.
28841463	7	8	theme	muscle	1228:1233	arg1	damage					1275:1280	oxidized DNA damage	1262:1280	oxidized DNA damage	1262:1280	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	8	theme	muscle	1228:1233	arg1	peroxidation					1248:1259	lipid peroxidation	1242:1259	lipid peroxidation	1242:1259	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	8	theme	muscle	1228:1233	arg1	cells					1235:1239	vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis	1212:1300	cells	1235:1239	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	8	theme	muscle	1228:1233	arg1	apoptosis					1292:1300	cell apoptosis	1287:1300	cell apoptosis	1287:1300	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	9	theme	oxidized	1262:1269	arg1	damage					1275:1280	oxidized DNA damage	1262:1280	oxidized DNA damage	1262:1280	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	9	theme	oxidized	1262:1269	arg1	cells					1235:1239	vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis	1212:1300	cells	1235:1239	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	6	10	theme	cell	1077:1080	arg1	infiltration					1082:1093	minimal intramural cell infiltration	1058:1093	minimal intramural cell infiltration	1058:1093	All grafts displayed antioxidant activity, were patent, and exhibited minimal intramural cell infiltration with varying degrees of calcification.
28841463	5	11	from	months	980:985	arg1	grafts					927:932	interposition grafts	913:932	interposition grafts in the abdominal aorta of adult rats at three months	913:985	Grafts were evaluated as interposition grafts in the abdominal aorta of adult rats at three months.
28841463	5	11	from	months	980:985	arg1	Grafts					888:893	Grafts	888:893	Grafts	888:893	Grafts were evaluated as interposition grafts in the abdominal aorta of adult rats at three months.
28841463	5	11	from	months	980:985	arg1	aorta					951:955	the abdominal aorta	937:955	the abdominal aorta of adult rats at three months	937:985	Grafts were evaluated as interposition grafts in the abdominal aorta of adult rats at three months.
28841463	5	12	theme	interposition	913:925	arg1	grafts					927:932	interposition grafts	913:932	interposition grafts in the abdominal aorta of adult rats at three months	913:985	Grafts were evaluated as interposition grafts in the abdominal aorta of adult rats at three months.
28841463	5	12	theme	interposition	913:925	arg1	Grafts					888:893	Grafts	888:893	Grafts	888:893	Grafts were evaluated as interposition grafts in the abdominal aorta of adult rats at three months.
28841463	6	13	theme	calcification	1119:1131	arg1	degrees					1108:1114	varying degrees	1100:1114	varying degrees of calcification	1100:1131	All grafts displayed antioxidant activity, were patent, and exhibited minimal intramural cell infiltration with varying degrees of calcification.
28841463	6	14	theme	minimal	1058:1064	arg1	infiltration					1082:1093	minimal intramural cell infiltration	1058:1093	minimal intramural cell infiltration	1058:1093	All grafts displayed antioxidant activity, were patent, and exhibited minimal intramural cell infiltration with varying degrees of calcification.
28841463	2	15	theme	thrombogenic	275:286	arg1	properties					317:326	thrombogenic and unpredictable remodeling properties	275:326	thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification	275:372	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	2	15	theme	thrombogenic	275:286	arg1	calcification					360:372	calcification	360:372	calcification	360:372	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	2	15	theme	thrombogenic	275:286	arg1	hyperplasia					344:354	intimal hyperplasia	336:354	intimal hyperplasia	336:354	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	3	16	theme	study	441:445	arg1	objective					423:431	The objective	419:431	The objective of this study	419:445	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	4	17	dep	Poly-ECM	788:795	arg1	2					785:785	2	785:785	2	785:785	Rat aortas were decellularized to create the following vascular grafts: 1) ECM hybridized with POCC (Poly-ECM), 2) Poly-ECM subsequently functionalized with heparin (Poly-ECM-Hep), and 3) non-modified vascular ECM.
28841463	4	18	dep	hybridized	752:761	arg1	1					745:745	1	745:745	1	745:745	Rat aortas were decellularized to create the following vascular grafts: 1) ECM hybridized with POCC (Poly-ECM), 2) Poly-ECM subsequently functionalized with heparin (Poly-ECM-Hep), and 3) non-modified vascular ECM.
28841463	7	19	theme	cell	1287:1290	arg1	cells					1235:1239	vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis	1212:1300	cells	1235:1239	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	19	theme	cell	1287:1290	arg1	apoptosis					1292:1300	cell apoptosis	1287:1300	cell apoptosis	1287:1300	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	3	20	theme	grafts	549:554	arg1	remodeling					493:502	remodeling	493:502	remodeling	493:502	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	3	20	theme	grafts	549:554	arg1	function					480:487	the long-term function	466:487	the long-term function	466:487	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	1	21	theme	high	175:178	arg1	patency					190:196	high long-term patency	175:196	high long-term patency	175:196	There is a need for off-the-shelf, small-diameter vascular grafts that are safe and exhibit high long-term patency.
28841463	7	22	theme	osteochondrogenic	1175:1191	arg1	differentiation					1193:1207	osteochondrogenic differentiation	1175:1207	osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis	1175:1300	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	9	23	theme	degenerative	1607:1618	arg1	changes					1620:1626	degenerative changes	1607:1626	degenerative changes	1607:1626	The incorporation of antioxidants into vascular grafts may be a viable strategy to inhibit degenerative changes.
28841463	1	24	theme	long-term	180:188	arg1	patency					190:196	high long-term patency	175:196	high long-term patency	175:196	There is a need for off-the-shelf, small-diameter vascular grafts that are safe and exhibit high long-term patency.
28841463	0	25	theme	Vascular	0:7	arg1	scaffolds					9:17	Vascular scaffolds	0:17	Vascular scaffolds with enhanced antioxidant activity	0:52	Vascular scaffolds with enhanced antioxidant activity inhibit graft calcification.
28841463	0	26	theme	enhanced	24:31	arg1	activity					45:52	enhanced antioxidant activity	24:52	enhanced antioxidant activity	24:52	Vascular scaffolds with enhanced antioxidant activity inhibit graft calcification.
28841463	7	27	dep	cells	1235:1239	arg1	damage					1275:1280	oxidized DNA damage	1262:1280	oxidized DNA damage	1262:1280	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	27	dep	cells	1235:1239	arg1	peroxidation					1248:1259	lipid peroxidation	1242:1259	lipid peroxidation	1242:1259	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	27	dep	cells	1235:1239	arg1	cells					1235:1239	vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis	1212:1300	cells	1235:1239	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	27	dep	cells	1235:1239	arg1	apoptosis					1292:1300	cell apoptosis	1287:1300	cell apoptosis	1287:1300	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	2	28	theme	clinical	405:412	arg1	use					414:416	their clinical use	399:416	their clinical use	399:416	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	3	29	theme	antioxidant	572:582	arg1	poly					584:587	antioxidant poly	572:587	antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC)	572:634	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	3	29	theme	antioxidant	572:582	arg1	POCC					630:633	POCC	630:633	POCC	630:633	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	6	30	theme	varying	1100:1106	arg1	degrees					1108:1114	varying degrees	1100:1114	varying degrees of calcification	1100:1131	All grafts displayed antioxidant activity, were patent, and exhibited minimal intramural cell infiltration with varying degrees of calcification.
28841463	3	31	theme	vascular	540:547	arg1	grafts					549:554	extracellular matrix (ECM)-based vascular grafts	507:554	extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin	507:670	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	8	32	theme	antioxidant	1463:1473	arg1	activity					1475:1482	their antioxidant activity	1457:1482	their antioxidant activity: Poly-ECM-Hep > ECM > Poly-ECM	1457:1513	The extent of calcification within grafts was inversely proportional to their antioxidant activity: Poly-ECM-Hep > ECM > Poly-ECM.
28841463	2	33	theme	remodeling	306:315	arg1	properties					317:326	thrombogenic and unpredictable remodeling properties	275:326	thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification	275:372	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	2	33	theme	remodeling	306:315	arg1	calcification					360:372	calcification	360:372	calcification	360:372	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	2	33	theme	remodeling	306:315	arg1	hyperplasia					344:354	intimal hyperplasia	336:354	intimal hyperplasia	336:354	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	2	34	used	used	241:244	arg2	grafts					258:263	vascular grafts	249:263	vascular grafts	249:263	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	2	34	used	used	241:244	arg2	tissues					214:220	Decellularized tissues	199:220	Decellularized tissues	199:220	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	7	35	theme	important	1317:1325	arg1	role					1327:1330	an important role	1314:1330	an important role for oxidative stress in the calcification of grafts	1314:1382	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	3	36	theme	matrix	521:526	arg1	grafts					549:554	extracellular matrix (ECM)-based vascular grafts	507:554	extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin	507:670	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	9	37	theme	antioxidants	1537:1548	arg1	strategy					1587:1594	a viable strategy	1578:1594	a viable strategy to inhibit degenerative changes	1578:1626	The incorporation of antioxidants into vascular grafts may be a viable strategy to inhibit degenerative changes.
28841463	9	37	theme	antioxidants	1537:1548	arg1	incorporation					1520:1532	The incorporation	1516:1532	The incorporation of antioxidants into vascular grafts	1516:1569	The incorporation of antioxidants into vascular grafts may be a viable strategy to inhibit degenerative changes.
28841463	7	38	theme	vascular	1212:1219	arg1	damage					1275:1280	oxidized DNA damage	1262:1280	oxidized DNA damage	1262:1280	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	38	theme	vascular	1212:1219	arg1	peroxidation					1248:1259	lipid peroxidation	1242:1259	lipid peroxidation	1242:1259	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	38	theme	vascular	1212:1219	arg1	cells					1235:1239	vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis	1212:1300	cells	1235:1239	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	38	theme	vascular	1212:1219	arg1	apoptosis					1292:1300	cell apoptosis	1287:1300	cell apoptosis	1287:1300	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	8	39	theme	calcification	1399:1411	arg1	proportional					1441:1452	proportional	1441:1452	proportional	1441:1452	The extent of calcification within grafts was inversely proportional to their antioxidant activity: Poly-ECM-Hep > ECM > Poly-ECM.
28841463	8	39	theme	calcification	1399:1411	arg1	extent					1389:1394	The extent	1385:1394	The extent of calcification within grafts	1385:1425	The extent of calcification within grafts was inversely proportional to their antioxidant activity: Poly-ECM-Hep > ECM > Poly-ECM.
28841463	0	40	theme	antioxidant	33:43	arg1	activity					45:52	enhanced antioxidant activity	24:52	enhanced antioxidant activity	24:52	Vascular scaffolds with enhanced antioxidant activity inhibit graft calcification.
28841463	7	41	theme	calcification	1143:1155	arg1	Areas					1134:1138	Areas	1134:1138	Areas of calcification	1134:1155	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	42	theme	oxidative	1336:1344	arg1	stress					1346:1351	oxidative stress	1336:1351	oxidative stress	1336:1351	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	3	43	theme	long-term	470:478	arg1	function					480:487	the long-term function	466:487	the long-term function	466:487	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	0	44	theme	graft	62:66	arg1	calcification					68:80	graft calcification	62:80	graft calcification	62:80	Vascular scaffolds with enhanced antioxidant activity inhibit graft calcification.
28841463	8	45	dep	activity	1475:1482	arg1	Poly-ECM-Hep > ECM > Poly-ECM					1485:1513	Poly-ECM-Hep > ECM > Poly-ECM	1485:1513	their antioxidant activity: Poly-ECM-Hep > ECM > Poly-ECM	1457:1513	The extent of calcification within grafts was inversely proportional to their antioxidant activity: Poly-ECM-Hep > ECM > Poly-ECM.
28841463	3	46	theme	immobilized	652:662	arg1	heparin					664:670	immobilized heparin	652:670	immobilized heparin	652:670	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	7	47	theme	grafts	1377:1382	arg1	calcification					1360:1372	the calcification	1356:1372	the calcification of grafts	1356:1382	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	3	48	theme	extracellular	507:519	arg1	ECM					529:531	ECM	529:531	ECM	529:531	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	3	48	theme	extracellular	507:519	arg1	matrix					521:526	extracellular matrix	507:526	extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin	507:670	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	9	49	theme	vascular	1555:1562	arg1	grafts					1564:1569	vascular grafts	1555:1569	vascular grafts	1555:1569	The incorporation of antioxidants into vascular grafts may be a viable strategy to inhibit degenerative changes.
28841463	4	50	theme	vascular	874:881	arg1	ECM					883:885	3) non-modified vascular ECM	858:885	3) non-modified vascular ECM	858:885	Rat aortas were decellularized to create the following vascular grafts: 1) ECM hybridized with POCC (Poly-ECM), 2) Poly-ECM subsequently functionalized with heparin (Poly-ECM-Hep), and 3) non-modified vascular ECM.
28841463	0	51	with	scaffolds	9:17	arg1	activity					45:52	enhanced antioxidant activity	24:52	enhanced antioxidant activity	24:52	Vascular scaffolds with enhanced antioxidant activity inhibit graft calcification.
28841463	1	52	theme	small-diameter	118:131	arg1	grafts					142:147	small-diameter vascular grafts	118:147	small-diameter vascular grafts that are safe and exhibit high long-term patency	118:196	There is a need for off-the-shelf, small-diameter vascular grafts that are safe and exhibit high long-term patency.
28841463	1	52	theme	small-diameter	118:131	arg1	off-the-shelf					103:115	off-the-shelf	103:115	off-the-shelf	103:115	There is a need for off-the-shelf, small-diameter vascular grafts that are safe and exhibit high long-term patency.
28841463	1	53	theme	vascular	133:140	arg1	grafts					142:147	small-diameter vascular grafts	118:147	small-diameter vascular grafts that are safe and exhibit high long-term patency	118:196	There is a need for off-the-shelf, small-diameter vascular grafts that are safe and exhibit high long-term patency.
28841463	1	53	theme	vascular	133:140	arg1	off-the-shelf					103:115	off-the-shelf	103:115	off-the-shelf	103:115	There is a need for off-the-shelf, small-diameter vascular grafts that are safe and exhibit high long-term patency.
28841463	6	54	theme	antioxidant	1009:1019	arg1	activity					1021:1028	antioxidant activity	1009:1028	antioxidant activity	1009:1028	All grafts displayed antioxidant activity, were patent, and exhibited minimal intramural cell infiltration with varying degrees of calcification.
28841463	5	55	theme	rats	966:969	arg1	aorta					951:955	the abdominal aorta	937:955	the abdominal aorta of adult rats at three months	937:985	Grafts were evaluated as interposition grafts in the abdominal aorta of adult rats at three months.
28841463	2	56	theme	Decellularized	199:212	arg1	grafts					258:263	vascular grafts	249:263	vascular grafts	249:263	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	2	56	theme	Decellularized	199:212	arg1	tissues					214:220	Decellularized tissues	199:220	Decellularized tissues	199:220	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	9	57	theme	viable	1580:1585	arg1	strategy					1587:1594	a viable strategy	1578:1594	a viable strategy to inhibit degenerative changes	1578:1626	The incorporation of antioxidants into vascular grafts may be a viable strategy to inhibit degenerative changes.
28841463	9	57	theme	viable	1580:1585	arg1	incorporation					1520:1532	The incorporation	1516:1532	The incorporation of antioxidants into vascular grafts	1516:1569	The incorporation of antioxidants into vascular grafts may be a viable strategy to inhibit degenerative changes.
28841463	7	58	from	role	1327:1330	arg1	calcification					1360:1372	the calcification	1356:1372	the calcification of grafts	1356:1382	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	2	59	theme	intimal	336:342	arg1	hyperplasia					344:354	intimal hyperplasia	336:354	intimal hyperplasia	336:354	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	5	60	from	grafts	927:932	arg1	months					980:985	three months	974:985	three months	974:985	Grafts were evaluated as interposition grafts in the abdominal aorta of adult rats at three months.
28841463	5	60	from	grafts	927:932	arg1	aorta					951:955	the abdominal aorta	937:955	the abdominal aorta of adult rats at three months	937:985	Grafts were evaluated as interposition grafts in the abdominal aorta of adult rats at three months.
28841463	7	61	theme	lipid	1242:1246	arg1	cells					1235:1239	vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis	1212:1300	cells	1235:1239	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	61	theme	lipid	1242:1246	arg1	peroxidation					1248:1259	lipid peroxidation	1242:1259	lipid peroxidation	1242:1259	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	5	62	theme	adult	960:964	arg1	rats					966:969	adult rats	960:969	adult rats	960:969	Grafts were evaluated as interposition grafts in the abdominal aorta of adult rats at three months.
28841463	7	63	theme	DNA	1271:1273	arg1	damage					1275:1280	oxidized DNA damage	1262:1280	oxidized DNA damage	1262:1280	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	7	63	theme	DNA	1271:1273	arg1	cells					1235:1239	vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis	1212:1300	cells	1235:1239	Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts.
28841463	4	64	theme	Rat	673:675	arg1	aortas					677:682	Rat aortas	673:682	Rat aortas	673:682	Rat aortas were decellularized to create the following vascular grafts: 1) ECM hybridized with POCC (Poly-ECM), 2) Poly-ECM subsequently functionalized with heparin (Poly-ECM-Hep), and 3) non-modified vascular ECM.
28841463	2	65	theme	vascular	249:256	arg1	grafts					258:263	vascular grafts	249:263	vascular grafts	249:263	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	2	65	theme	vascular	249:256	arg1	tissues					214:220	Decellularized tissues	199:220	Decellularized tissues	199:220	Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use.
28841463	3	66	theme	-based	533:538	arg1	grafts					549:554	extracellular matrix (ECM)-based vascular grafts	507:554	extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin	507:670	The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylene-citrate-co-cysteine) (POCC) with or without immobilized heparin.
28841463	4	67	theme	vascular	728:735	arg1	grafts					737:742	the following vascular grafts	714:742	the following vascular grafts	714:742	Rat aortas were decellularized to create the following vascular grafts: 1) ECM hybridized with POCC (Poly-ECM), 2) Poly-ECM subsequently functionalized with heparin (Poly-ECM-Hep), and 3) non-modified vascular ECM.
29080509	6	0	theme	porous	955:960	arg1	scaffold					972:979	a porous cellulose scaffold	953:979	a porous cellulose scaffold	953:979	The facile preparation method ensured uniform morphology along the layers and stand for the high ionic-liquid loading in a porous cellulose scaffold.
29080509	3	1	theme	step-voltage	494:505	arg1	values					507:512	±500mV step-voltage values	487:512	±500mV step-voltage values	487:512	The CEL-iGEL composites undergo reversible bending already at ±500mV step-voltage values.
29080509	7	2	theme	CEL-iGEL	1228:1235	arg1	actuators					1237:1245	CEL-iGEL actuators	1228:1245	CEL-iGEL actuators	1228:1245	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	2	3	theme	artificial	328:337	arg1	application					346:356	soft artificial muscle application	323:356	soft artificial muscle application	323:356	The natural cellulose-based ionogels (CEL-iGEL) hold a great potential for soft artificial muscle application, due to its flexibility, low driving voltage and biocompatibility.
29080509	4	4	theme	ionic	563:567	arg1	conductivity					569:580	the voltage applied and high ionic conductivity	534:580	the voltage applied and high ionic conductivity of membranous actuator	534:603	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	5	5	theme	cellulose	731:739	arg1	films					750:754	The CEL-iGEL supported cellulose actuator films	708:754	The CEL-iGEL supported cellulose actuator films	708:754	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	5	5	theme	cellulose	731:739	arg1	cast					761:764	cast	761:764	cast	761:764	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	7	6	theme	difference	1125:1134	arg1	values					1136:1141	the max strain difference values	1110:1141	the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis	1110:1194	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	1	7	theme	Rapid	56:60	arg1	development					62:72	Rapid development	56:72	Rapid development of soft micromanipulation techniques for human friendly electronics	56:140	Rapid development of soft micromanipulation techniques for human friendly electronics has raised the demand for the devices to be able to carry out mechanical work on a micro- and macroscale.
29080509	4	8	theme	membranous	585:594	arg1	actuator					596:603	membranous actuator	585:603	membranous actuator	585:603	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	5	9	theme	actuator	741:748	arg1	films					750:754	The CEL-iGEL supported cellulose actuator films	708:754	The CEL-iGEL supported cellulose actuator films	708:754	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	5	9	theme	actuator	741:748	arg1	cast					761:764	cast	761:764	cast	761:764	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	5	10	from	inversion	814:822	arg1	H2O					827:829	H2O	827:829	H2O	827:829	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	2	11	theme	muscle	339:344	arg1	application					346:356	soft artificial muscle application	323:356	soft artificial muscle application	323:356	The natural cellulose-based ionogels (CEL-iGEL) hold a great potential for soft artificial muscle application, due to its flexibility, low driving voltage and biocompatibility.
29080509	2	12	theme	soft	323:326	arg1	application					346:356	soft artificial muscle application	323:356	soft artificial muscle application	323:356	The natural cellulose-based ionogels (CEL-iGEL) hold a great potential for soft artificial muscle application, due to its flexibility, low driving voltage and biocompatibility.
29080509	7	13	theme	max	1114:1116	arg1	values					1136:1141	the max strain difference values	1110:1141	the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis	1110:1194	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	3	14	theme	reversible	457:466	arg1	bending					468:474	reversible bending	457:474	reversible bending	457:474	The CEL-iGEL composites undergo reversible bending already at ±500mV step-voltage values.
29080509	2	15	theme	cellulose-based	260:274	arg1	ionogels					276:283	The natural cellulose-based ionogels	248:283	The natural cellulose-based ionogels (CEL-iGEL)	248:294	The natural cellulose-based ionogels (CEL-iGEL) hold a great potential for soft artificial muscle application, due to its flexibility, low driving voltage and biocompatibility.
29080509	2	15	theme	cellulose-based	260:274	arg1	CEL-iGEL					286:293	CEL-iGEL	286:293	CEL-iGEL	286:293	The natural cellulose-based ionogels (CEL-iGEL) hold a great potential for soft artificial muscle application, due to its flexibility, low driving voltage and biocompatibility.
29080509	5	16	theme	[OAc	789:792	arg1	solution					795:802	cellulose-[EMIm][OAc] solution	773:802	cellulose-[EMIm][OAc] solution	773:802	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	4	17	dep	applied	546:552	arg1	voltage					538:544	voltage	538:544	voltage	538:544	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	7	18	theme	strain	1118:1123	arg1	values					1136:1141	the max strain difference values	1110:1141	the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis	1110:1194	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	6	19	theme	preparation	843:853	arg1	method					855:860	The facile preparation method	832:860	The facile preparation method	832:860	The facile preparation method ensured uniform morphology along the layers and stand for the high ionic-liquid loading in a porous cellulose scaffold.
29080509	2	20	theme	natural	252:258	arg1	ionogels					276:283	The natural cellulose-based ionogels	248:283	The natural cellulose-based ionogels (CEL-iGEL)	248:294	The natural cellulose-based ionogels (CEL-iGEL) hold a great potential for soft artificial muscle application, due to its flexibility, low driving voltage and biocompatibility.
29080509	2	20	theme	natural	252:258	arg1	CEL-iGEL					286:293	CEL-iGEL	286:293	CEL-iGEL	286:293	The natural cellulose-based ionogels (CEL-iGEL) hold a great potential for soft artificial muscle application, due to its flexibility, low driving voltage and biocompatibility.
29080509	4	21	theme	cellulose	646:654	arg1	dissolution					631:641	a complete dissolution	620:641	a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc]	620:705	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	7	22	theme	Electrochemical	1171:1185	arg1	analysis					1187:1194	2 V. Electrochemical analysis	1166:1194	2 V. Electrochemical analysis	1166:1194	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	1	23	theme	micromanipulation	82:98	arg1	techniques					100:109	soft micromanipulation techniques	77:109	soft micromanipulation techniques for human friendly electronics	77:140	Rapid development of soft micromanipulation techniques for human friendly electronics has raised the demand for the devices to be able to carry out mechanical work on a micro- and macroscale.
29080509	0	24	theme	Natural	0:6	arg1	cellulose					8:16	Natural cellulose	0:16	Natural cellulose	0:16	Natural cellulose ionogels for soft artificial muscles.
29080509	4	25	theme	applied	546:552	arg1	conductivity					569:580	the voltage applied and high ionic conductivity	534:580	the voltage applied and high ionic conductivity of membranous actuator	534:603	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	5	26	dep	[OAc	789:792	arg1	cellulose-[EMIm					773:787	cellulose-[EMIm	773:787	cellulose-[EMIm	773:787	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	1	27	theme	techniques	100:109	arg1	development					62:72	Rapid development	56:72	Rapid development of soft micromanipulation techniques for human friendly electronics	56:140	Rapid development of soft micromanipulation techniques for human friendly electronics has raised the demand for the devices to be able to carry out mechanical work on a micro- and macroscale.
29080509	4	28	theme	1-ethyl-3-methylimidazolium	659:685	arg1	acetate					687:693	1-ethyl-3-methylimidazolium acetate	659:693	1-ethyl-3-methylimidazolium acetate [EMIm][OAc]	659:705	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	5	29	theme	phase	808:812	arg1	inversion					814:822	phase inversion	808:822	phase inversion in H2O	808:829	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	4	30	from	dissolution	631:641	arg1	acetate					687:693	1-ethyl-3-methylimidazolium acetate	659:693	1-ethyl-3-methylimidazolium acetate [EMIm][OAc]	659:705	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	4	31	theme	complete	622:629	arg1	dissolution					631:641	a complete dissolution	620:641	a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc]	620:705	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	7	32	theme	V.	1168:1169	arg1	analysis					1187:1194	2 V. Electrochemical analysis	1166:1194	2 V. Electrochemical analysis	1166:1194	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	4	33	theme	high	558:561	arg1	conductivity					569:580	the voltage applied and high ionic conductivity	534:580	the voltage applied and high ionic conductivity of membranous actuator	534:603	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	6	34	theme	uniform	870:876	arg1	morphology					878:887	uniform morphology	870:887	uniform morphology	870:887	The facile preparation method ensured uniform morphology along the layers and stand for the high ionic-liquid loading in a porous cellulose scaffold.
29080509	1	35	theme	human	115:119	arg1	electronics					130:140	human friendly electronics	115:140	human friendly electronics	115:140	Rapid development of soft micromanipulation techniques for human friendly electronics has raised the demand for the devices to be able to carry out mechanical work on a micro- and macroscale.
29080509	2	36	theme	driving	387:393	arg1	voltage					395:401	low driving voltage	383:401	low driving voltage	383:401	The natural cellulose-based ionogels (CEL-iGEL) hold a great potential for soft artificial muscle application, due to its flexibility, low driving voltage and biocompatibility.
29080509	0	37	theme	artificial	36:45	arg1	muscles					47:53	soft artificial muscles	31:53	soft artificial muscles	31:53	Natural cellulose ionogels for soft artificial muscles.
29080509	5	38	theme	CEL-iGEL	712:719	arg1	films					750:754	The CEL-iGEL supported cellulose actuator films	708:754	The CEL-iGEL supported cellulose actuator films	708:754	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	5	38	theme	CEL-iGEL	712:719	arg1	cast					761:764	cast	761:764	cast	761:764	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	3	39	theme	CEL-iGEL	429:436	arg1	composites					438:447	The CEL-iGEL composites	425:447	The CEL-iGEL composites	425:447	The CEL-iGEL composites undergo reversible bending already at ±500mV step-voltage values.
29080509	2	40	theme	low	383:385	arg1	voltage					395:401	low driving voltage	383:401	low driving voltage	383:401	The natural cellulose-based ionogels (CEL-iGEL) hold a great potential for soft artificial muscle application, due to its flexibility, low driving voltage and biocompatibility.
29080509	0	41	theme	soft	31:34	arg1	muscles					47:53	soft artificial muscles	31:53	soft artificial muscles	31:53	Natural cellulose ionogels for soft artificial muscles.
29080509	4	42	dep	[EMIm	695:699	arg1	[OAc					701:704	[OAc	701:704	[OAc	701:704	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	7	43	theme	mathematical	1386:1397	arg1	model					1399:1403	a mathematical model	1384:1403	a mathematical model	1384:1403	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	1	44	theme	mechanical	204:213	arg1	work					215:218	mechanical work	204:218	mechanical work on a micro- and macroscale	204:245	Rapid development of soft micromanipulation techniques for human friendly electronics has raised the demand for the devices to be able to carry out mechanical work on a micro- and macroscale.
29080509	4	45	theme	fast	517:520	arg1	response					522:529	A fast response	515:529	A fast response to the voltage applied and high ionic conductivity of membranous actuator	515:603	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	7	46	theme	exponential	1059:1069	arg1	dependence					1071:1080	exponential dependence	1059:1080	exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis	1059:1194	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	6	47	theme	cellulose	962:970	arg1	scaffold					972:979	a porous cellulose scaffold	953:979	a porous cellulose scaffold	953:979	The facile preparation method ensured uniform morphology along the layers and stand for the high ionic-liquid loading in a porous cellulose scaffold.
29080509	7	48	theme	CEL-iGEL	1033:1040	arg1	actuators					1042:1050	the CEL-iGEL actuators	1029:1050	the CEL-iGEL actuators	1029:1050	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	1	49	theme	friendly	121:128	arg1	electronics					130:140	human friendly electronics	115:140	human friendly electronics	115:140	Rapid development of soft micromanipulation techniques for human friendly electronics has raised the demand for the devices to be able to carry out mechanical work on a micro- and macroscale.
29080509	5	50	theme	supported	721:729	arg1	films					750:754	The CEL-iGEL supported cellulose actuator films	708:754	The CEL-iGEL supported cellulose actuator films	708:754	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	5	50	theme	supported	721:729	arg1	cast					761:764	cast	761:764	cast	761:764	The CEL-iGEL supported cellulose actuator films were cast out of cellulose-[EMIm][OAc] solution via phase inversion in H2O.
29080509	6	51	theme	ionic-liquid	929:940	arg1	loading					942:948	the high ionic-liquid loading	920:948	the high ionic-liquid loading	920:948	The facile preparation method ensured uniform morphology along the layers and stand for the high ionic-liquid loading in a porous cellulose scaffold.
29080509	6	52	theme	facile	836:841	arg1	method					855:860	The facile preparation method	832:860	The facile preparation method	832:860	The facile preparation method ensured uniform morphology along the layers and stand for the high ionic-liquid loading in a porous cellulose scaffold.
29080509	7	53	dep	0.6	1158:1160	arg1	to					1155:1156	to	1155:1156	to	1155:1156	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	3	54	theme	±500mV	487:492	arg1	values					507:512	±500mV step-voltage values	487:512	±500mV step-voltage values	487:512	The CEL-iGEL composites undergo reversible bending already at ±500mV step-voltage values.
29080509	6	55	theme	high	924:927	arg1	loading					942:948	the high ionic-liquid loading	920:948	the high ionic-liquid loading	920:948	The facile preparation method ensured uniform morphology along the layers and stand for the high ionic-liquid loading in a porous cellulose scaffold.
29080509	1	56	from	work	215:218	arg1	macroscale					236:245	macroscale	236:245	macroscale	236:245	Rapid development of soft micromanipulation techniques for human friendly electronics has raised the demand for the devices to be able to carry out mechanical work on a micro- and macroscale.
29080509	1	56	from	work	215:218	arg1	micro-					225:230	micro-	225:230	micro-	225:230	Rapid development of soft micromanipulation techniques for human friendly electronics has raised the demand for the devices to be able to carry out mechanical work on a micro- and macroscale.
29080509	7	57	theme	step	1363:1366	arg1	voltages					1374:1381	various step input voltages	1355:1381	various step input voltages	1355:1381	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	4	58	dep	acetate	687:693	arg1	[EMIm					695:699	[EMIm	695:699	[EMIm	695:699	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	7	59	theme	voltage	1279:1285	arg1	value					1287:1291	the safe working voltage value	1262:1291	the safe working voltage value	1262:1291	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	1	60	theme	soft	77:80	arg1	techniques					100:109	soft micromanipulation techniques	77:109	soft micromanipulation techniques for human friendly electronics	77:140	Rapid development of soft micromanipulation techniques for human friendly electronics has raised the demand for the devices to be able to carry out mechanical work on a micro- and macroscale.
29080509	7	61	theme	input	1368:1372	arg1	voltages					1374:1381	various step input voltages	1355:1381	various step input voltages	1355:1381	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	2	62	theme	great	303:307	arg1	potential					309:317	a great potential	301:317	a great potential for soft artificial muscle application	301:356	The natural cellulose-based ionogels (CEL-iGEL) hold a great potential for soft artificial muscle application, due to its flexibility, low driving voltage and biocompatibility.
29080509	4	63	theme	actuator	596:603	arg1	conductivity					569:580	the voltage applied and high ionic conductivity	534:580	the voltage applied and high ionic conductivity of membranous actuator	534:603	A fast response to the voltage applied and high ionic conductivity of membranous actuator is achieved by a complete dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate [EMIm][OAc].
29080509	7	64	theme	actuators	1237:1245	arg1	stability					1215:1223	the good stability	1206:1223	the good stability of CEL-iGEL actuators	1206:1245	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	7	65	theme	electromechanical	993:1009	arg1	characterization					1011:1026	the electromechanical characterization	989:1026	the electromechanical characterization	989:1026	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	7	66	theme	safe	1266:1269	arg1	value					1287:1291	the safe working voltage value	1262:1291	the safe working voltage value	1262:1291	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	7	67	theme	good	1210:1213	arg1	stability					1215:1223	the good stability	1206:1223	the good stability of CEL-iGEL actuators	1206:1245	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	7	68	theme	various	1355:1361	arg1	voltages					1374:1381	various step input voltages	1355:1381	various step input voltages	1355:1381	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	7	69	theme	working	1271:1277	arg1	value					1287:1291	the safe working voltage value	1262:1291	the safe working voltage value	1262:1291	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	7	70	dep	showed	1052:1057	arg1	confirmed					1196:1204	confirmed	1196:1204	confirmed the good stability of CEL-iGEL actuators	1196:1245	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
29080509	7	70	dep	showed	1052:1057	arg1	determined					1251:1260	determined	1251:1260	determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages	1251:1381	During the electromechanical characterization, the CEL-iGEL actuators showed exponential dependence to the voltage applied with the max strain difference values reaching up to 0.6% at 2 V. Electrochemical analysis confirmed the good stability of CEL-iGEL actuators and determined the safe working voltage value to be below 2.5V. To predict and estimate the deformation for various step input voltages, a mathematical model was proposed.
28126456	5	0	with	SEM	1028:1030	arg1	EDAX					1037:1040	EDAX	1037:1040	EDAX	1037:1040	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	4	1	theme	oil	894:896	arg1	concentration					898:910	initial oil concentration	886:910	initial oil concentration	886:910	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	1	2	from	emulsion	183:190	arg1	recovery					149:156	The recovery	145:156	The recovery of oil from oil-in-water emulsion	145:190	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	1	3	theme	oil	161:163	arg1	recovery					149:156	The recovery	145:156	The recovery of oil from oil-in-water emulsion	145:190	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	9	4	theme	technology	1583:1592	arg1	growth					1561:1566	the growth	1557:1566	the growth of oil removal technology	1557:1592	This work provides an apparent proposal for the growth of oil removal technology.
28126456	4	5	theme	initial	886:892	arg1	concentration					898:910	initial oil concentration	886:910	initial oil concentration	886:910	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	5	6	theme	as-synthesized	933:946	arg1	CS-LDHCs					948:955	The as-synthesized CS-LDHCs	929:955	The as-synthesized CS-LDHCs	929:955	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	2	7	theme	as-prepared	537:547	arg1	LDH					549:551	as-prepared LDH	537:551	as-prepared LDH	537:551	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	0	8	theme	oil	103:105	arg1	particles					107:115	oil particles	103:115	oil particles	103:115	Synthesis and characterization of chitosan/Mg-Al layered double hydroxide composite for the removal of oil particles from oil-in-water emulsion.
28126456	9	9	theme	apparent	1535:1542	arg1	proposal					1544:1551	an apparent proposal	1532:1551	an apparent proposal for the growth of oil removal technology	1532:1592	This work provides an apparent proposal for the growth of oil removal technology.
28126456	6	10	theme	isotherm	1236:1243	arg1	models					1245:1250	isotherm models	1236:1250	isotherm models	1236:1250	To find out the best fit for the sorption process, the obtained adsorption equilibrium data was explained with Freundlich, Langmuir, Dubinin-Radushkevich and Tempkin isotherm models.
28126456	4	11	theme	experiment	777:786	arg1	study					788:792	Batch experiment study	771:792	Batch experiment study	771:792	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	3	12	theme	properties	601:610	arg1	enhancement					575:585	The enhancement	571:585	The enhancement of adsorption properties by CS-LDHCs material	571:631	The enhancement of adsorption properties by CS-LDHCs material were attributed to the high content of LDH in chitosan, which makes the material more effective towards immobilization of oily particles.
28126456	3	13	theme	oily	755:758	arg1	particles					760:768	oily particles	755:768	oily particles	755:768	The enhancement of adsorption properties by CS-LDHCs material were attributed to the high content of LDH in chitosan, which makes the material more effective towards immobilization of oily particles.
28126456	5	14	with	analysis	1060:1067	arg1	EDAX					1037:1040	EDAX	1037:1040	EDAX	1037:1040	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	15	with	XRD	1043:1045	arg1	EDAX					1037:1040	EDAX	1037:1040	EDAX	1037:1040	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	3	16	theme	particles	760:768	arg1	immobilization					737:750	immobilization	737:750	immobilization of oily particles	737:768	The enhancement of adsorption properties by CS-LDHCs material were attributed to the high content of LDH in chitosan, which makes the material more effective towards immobilization of oily particles.
28126456	4	17	theme	Batch	771:775	arg1	study					788:792	Batch experiment study	771:792	Batch experiment study	771:792	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	7	18	from	parameters	1364:1373	arg1	values					1338:1343	ΔG°, ΔH° and ΔS° values	1321:1343	ΔG°, ΔH° and ΔS° values from thermodynamic parameters	1321:1373	The mechanism of adsorption process was demonstrated by calculating ΔG°, ΔH° and ΔS° values from thermodynamic parameters in order to understand the nature of sorption process.
28126456	1	19	theme	oil-in-water	170:181	arg1	emulsion					183:190	oil-in-water emulsion	170:190	oil-in-water emulsion	170:190	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	0	20	theme	particles	107:115	arg1	removal					92:98	the removal	88:98	the removal of oil particles from oil-in-water emulsion	88:142	Synthesis and characterization of chitosan/Mg-Al layered double hydroxide composite for the removal of oil particles from oil-in-water emulsion.
28126456	5	21	with	FTIR	1022:1025	arg1	EDAX					1037:1040	EDAX	1037:1040	EDAX	1037:1040	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	22	theme	various	978:984	arg1	analysis					1060:1067	DSC analysis	1056:1067	DSC analysis	1056:1067	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	22	theme	various	978:984	arg1	viz.					1016:1019	viz.	1016:1019	viz.	1016:1019	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	22	theme	various	978:984	arg1	FTIR					1022:1025	FTIR	1022:1025	FTIR	1022:1025	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	22	theme	various	978:984	arg1	XRD					1043:1045	XRD	1043:1045	XRD	1043:1045	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	22	theme	various	978:984	arg1	TGA					1048:1050	TGA	1048:1050	TGA	1048:1050	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	22	theme	various	978:984	arg1	SEM					1028:1030	SEM	1028:1030	SEM with EDAX	1028:1040	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	22	theme	various	978:984	arg1	techniques					1005:1014	various spectro analytical techniques	978:1014	various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis	978:1067	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	7	23	theme	process	1421:1427	arg1	nature					1402:1407	the nature	1398:1407	the nature of sorption process	1398:1427	The mechanism of adsorption process was demonstrated by calculating ΔG°, ΔH° and ΔS° values from thermodynamic parameters in order to understand the nature of sorption process.
28126456	2	24	from	pH	520:521	arg1	material					436:443	the synthesized material	420:443	the synthesized material	420:443	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	2	24	from	pH	520:521	arg1	effective					449:457	effective	449:457	effective	449:457	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	6	25	theme	best	1086:1089	arg1	fit					1091:1093	the best fit	1082:1093	the best fit for the sorption process	1082:1118	To find out the best fit for the sorption process, the obtained adsorption equilibrium data was explained with Freundlich, Langmuir, Dubinin-Radushkevich and Tempkin isotherm models.
28126456	2	26	theme	adsorption	356:365	arg1	efficiency					367:376	better adsorption efficiency	349:376	Resulting better adsorption efficiency of CS-LDHCs	339:388	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	3	27	from	content	661:667	arg1	chitosan					679:686	chitosan	679:686	chitosan	679:686	The enhancement of adsorption properties by CS-LDHCs material were attributed to the high content of LDH in chitosan, which makes the material more effective towards immobilization of oily particles.
28126456	2	28	theme	synthesized	424:434	arg1	material					436:443	the synthesized material	420:443	the synthesized material	420:443	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	2	28	theme	synthesized	424:434	arg1	effective					449:457	effective	449:457	effective	449:457	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	2	29	theme	oil-in-water	488:499	arg1	emulsion					501:508	oil-in-water emulsion	488:508	oil-in-water emulsion	488:508	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	5	30	with	TGA	1048:1050	arg1	EDAX					1037:1040	EDAX	1037:1040	EDAX	1037:1040	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	9	31	theme	removal	1575:1581	arg1	technology					1583:1592	oil removal technology	1571:1592	oil removal technology	1571:1592	This work provides an apparent proposal for the growth of oil removal technology.
28126456	2	32	theme	better	349:354	arg1	efficiency					367:376	better adsorption efficiency	349:376	Resulting better adsorption efficiency of CS-LDHCs	339:388	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	7	33	theme	ΔS°	1334:1336	arg1	values					1338:1343	ΔG°, ΔH° and ΔS° values	1321:1343	ΔG°, ΔH° and ΔS° values from thermodynamic parameters	1321:1373	The mechanism of adsorption process was demonstrated by calculating ΔG°, ΔH° and ΔS° values from thermodynamic parameters in order to understand the nature of sorption process.
28126456	5	34	with	viz.	1016:1019	arg1	EDAX					1037:1040	EDAX	1037:1040	EDAX	1037:1040	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	1	35	theme	single	307:312	arg1	method					331:336	a single co-precipitation method	305:336	a single co-precipitation method	305:336	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	6	36	theme	adsorption	1134:1143	arg1	data					1157:1160	the obtained adsorption equilibrium data	1121:1160	the obtained adsorption equilibrium data	1121:1160	To find out the best fit for the sorption process, the obtained adsorption equilibrium data was explained with Freundlich, Langmuir, Dubinin-Radushkevich and Tempkin isotherm models.
28126456	2	37	theme	Resulting	339:347	arg1	efficiency					367:376	better adsorption efficiency	349:376	Resulting better adsorption efficiency of CS-LDHCs	339:388	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	2	38	theme	oil	469:471	arg1	particles					473:481	oil particles	469:481	oil particles	469:481	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	5	39	theme	spectro	986:992	arg1	analysis					1060:1067	DSC analysis	1056:1067	DSC analysis	1056:1067	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	39	theme	spectro	986:992	arg1	viz.					1016:1019	viz.	1016:1019	viz.	1016:1019	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	39	theme	spectro	986:992	arg1	FTIR					1022:1025	FTIR	1022:1025	FTIR	1022:1025	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	39	theme	spectro	986:992	arg1	XRD					1043:1045	XRD	1043:1045	XRD	1043:1045	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	39	theme	spectro	986:992	arg1	TGA					1048:1050	TGA	1048:1050	TGA	1048:1050	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	39	theme	spectro	986:992	arg1	SEM					1028:1030	SEM	1028:1030	SEM with EDAX	1028:1040	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	39	theme	spectro	986:992	arg1	techniques					1005:1014	various spectro analytical techniques	978:1014	various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis	978:1067	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	1	40	theme	co-precipitation	314:329	arg1	method					331:336	a single co-precipitation method	305:336	a single co-precipitation method	305:336	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	0	41	theme	oil-in-water	122:133	arg1	emulsion					135:142	oil-in-water emulsion	122:142	oil-in-water emulsion	122:142	Synthesis and characterization of chitosan/Mg-Al layered double hydroxide composite for the removal of oil particles from oil-in-water emulsion.
28126456	8	42	theme	removal	1466:1472	arg1	representation					1444:1457	The schematic representation	1430:1457	The schematic representation of oil removal using CS-LDHCs	1430:1487	The schematic representation of oil removal using CS-LDHCs was explored in detail.
28126456	3	43	theme	adsorption	590:599	arg1	properties					601:610	adsorption properties	590:610	adsorption properties	590:610	The enhancement of adsorption properties by CS-LDHCs material were attributed to the high content of LDH in chitosan, which makes the material more effective towards immobilization of oily particles.
28126456	2	44	theme	acidic	513:518	arg1	pH					524:525	pH 3.0	524:529	pH 3.0	524:529	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	2	44	theme	acidic	513:518	arg1	pH					520:521	acidic pH	513:521	acidic pH (pH 3.0)	513:530	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	7	45	theme	ΔH°	1326:1328	arg1	values					1338:1343	ΔG°, ΔH° and ΔS° values	1321:1343	ΔG°, ΔH° and ΔS° values from thermodynamic parameters	1321:1373	The mechanism of adsorption process was demonstrated by calculating ΔG°, ΔH° and ΔS° values from thermodynamic parameters in order to understand the nature of sorption process.
28126456	0	46	theme	layered	49:55	arg1	composite					74:82	chitosan/Mg-Al layered double hydroxide composite	34:82	chitosan/Mg-Al layered double hydroxide composite	34:82	Synthesis and characterization of chitosan/Mg-Al layered double hydroxide composite for the removal of oil particles from oil-in-water emulsion.
28126456	7	47	theme	ΔG°	1321:1323	arg1	values					1338:1343	ΔG°, ΔH° and ΔS° values	1321:1343	ΔG°, ΔH° and ΔS° values from thermodynamic parameters	1321:1373	The mechanism of adsorption process was demonstrated by calculating ΔG°, ΔH° and ΔS° values from thermodynamic parameters in order to understand the nature of sorption process.
28126456	8	48	theme	oil	1462:1464	arg1	removal					1466:1472	oil removal	1462:1472	oil removal using CS-LDHCs	1462:1487	The schematic representation of oil removal using CS-LDHCs was explored in detail.
28126456	1	49	theme	chitosan/magnesium-aluminium	220:247	arg1	CS-LDHCs					292:299	CS-LDHCs	292:299	CS-LDHCs	292:299	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	1	49	theme	chitosan/magnesium-aluminium	220:247	arg1	composite					281:289	chitosan/magnesium-aluminium layered double hydroxide hybrid composite	220:289	chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs)	220:300	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	0	50	theme	chitosan/Mg-Al	34:47	arg1	composite					74:82	chitosan/Mg-Al layered double hydroxide composite	34:82	chitosan/Mg-Al layered double hydroxide composite	34:82	Synthesis and characterization of chitosan/Mg-Al layered double hydroxide composite for the removal of oil particles from oil-in-water emulsion.
28126456	0	51	from	emulsion	135:142	arg1	removal					92:98	the removal	88:98	the removal of oil particles from oil-in-water emulsion	88:142	Synthesis and characterization of chitosan/Mg-Al layered double hydroxide composite for the removal of oil particles from oil-in-water emulsion.
28126456	1	52	theme	layered	249:255	arg1	CS-LDHCs					292:299	CS-LDHCs	292:299	CS-LDHCs	292:299	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	1	52	theme	layered	249:255	arg1	composite					281:289	chitosan/magnesium-aluminium layered double hydroxide hybrid composite	220:289	chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs)	220:300	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	0	53	theme	hydroxide	64:72	arg1	composite					74:82	chitosan/Mg-Al layered double hydroxide composite	34:82	chitosan/Mg-Al layered double hydroxide composite	34:82	Synthesis and characterization of chitosan/Mg-Al layered double hydroxide composite for the removal of oil particles from oil-in-water emulsion.
28126456	2	54	theme	raw	557:559	arg1	chitosan					561:568	raw chitosan	557:568	raw chitosan	557:568	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	3	55	theme	LDH	672:674	arg1	content					661:667	the high content	652:667	the high content of LDH in chitosan, which makes the material more effective towards immobilization of oily particles	652:768	The enhancement of adsorption properties by CS-LDHCs material were attributed to the high content of LDH in chitosan, which makes the material more effective towards immobilization of oily particles.
28126456	3	56	theme	CS-LDHCs	615:622	arg1	material					624:631	CS-LDHCs material	615:631	CS-LDHCs material	615:631	The enhancement of adsorption properties by CS-LDHCs material were attributed to the high content of LDH in chitosan, which makes the material more effective towards immobilization of oily particles.
28126456	6	57	theme	obtained	1125:1132	arg1	data					1157:1160	the obtained adsorption equilibrium data	1121:1160	the obtained adsorption equilibrium data	1121:1160	To find out the best fit for the sorption process, the obtained adsorption equilibrium data was explained with Freundlich, Langmuir, Dubinin-Radushkevich and Tempkin isotherm models.
28126456	0	58	theme	double	57:62	arg1	composite					74:82	chitosan/Mg-Al layered double hydroxide composite	34:82	chitosan/Mg-Al layered double hydroxide composite	34:82	Synthesis and characterization of chitosan/Mg-Al layered double hydroxide composite for the removal of oil particles from oil-in-water emulsion.
28126456	5	59	theme	DSC	1056:1058	arg1	techniques					1005:1014	various spectro analytical techniques	978:1014	various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis	978:1067	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	59	theme	DSC	1056:1058	arg1	analysis					1060:1067	DSC analysis	1056:1067	DSC analysis	1056:1067	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	6	60	theme	equilibrium	1145:1155	arg1	data					1157:1160	the obtained adsorption equilibrium data	1121:1160	the obtained adsorption equilibrium data	1121:1160	To find out the best fit for the sorption process, the obtained adsorption equilibrium data was explained with Freundlich, Langmuir, Dubinin-Radushkevich and Tempkin isotherm models.
28126456	2	61	theme	CS-LDHCs	381:388	arg1	efficiency					367:376	better adsorption efficiency	349:376	Resulting better adsorption efficiency of CS-LDHCs	339:388	Resulting better adsorption efficiency of CS-LDHCs could be observed, indicating the synthesized material was effective to adsorb oil particles from oil-in-water emulsion at acidic pH (pH 3.0) than as-prepared LDH and raw chitosan.
28126456	8	62	theme	schematic	1434:1442	arg1	representation					1444:1457	The schematic representation	1430:1457	The schematic representation of oil removal using CS-LDHCs	1430:1487	The schematic representation of oil removal using CS-LDHCs was explored in detail.
28126456	4	63	theme	different	825:833	arg1	pH					876:877	pH	876:877	pH	876:877	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	4	63	theme	different	825:833	arg1	parameters					851:860	different physicochemical parameters	825:860	different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature	825:926	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	4	63	theme	different	825:833	arg1	dose					880:883	dose	880:883	dose	880:883	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	4	63	theme	different	825:833	arg1	temperature					916:926	temperature	916:926	temperature	916:926	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	4	63	theme	different	825:833	arg1	time					870:873	time	870:873	time	870:873	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	4	63	theme	different	825:833	arg1	concentration					898:910	initial oil concentration	886:910	initial oil concentration	886:910	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	3	64	theme	high	656:659	arg1	content					661:667	the high content	652:667	the high content of LDH in chitosan, which makes the material more effective towards immobilization of oily particles	652:768	The enhancement of adsorption properties by CS-LDHCs material were attributed to the high content of LDH in chitosan, which makes the material more effective towards immobilization of oily particles.
28126456	7	65	theme	process	1281:1287	arg1	mechanism					1257:1265	The mechanism	1253:1265	The mechanism of adsorption process	1253:1287	The mechanism of adsorption process was demonstrated by calculating ΔG°, ΔH° and ΔS° values from thermodynamic parameters in order to understand the nature of sorption process.
28126456	5	66	theme	analytical	994:1003	arg1	analysis					1060:1067	DSC analysis	1056:1067	DSC analysis	1056:1067	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	66	theme	analytical	994:1003	arg1	viz.					1016:1019	viz.	1016:1019	viz.	1016:1019	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	66	theme	analytical	994:1003	arg1	FTIR					1022:1025	FTIR	1022:1025	FTIR	1022:1025	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	66	theme	analytical	994:1003	arg1	XRD					1043:1045	XRD	1043:1045	XRD	1043:1045	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	66	theme	analytical	994:1003	arg1	TGA					1048:1050	TGA	1048:1050	TGA	1048:1050	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	66	theme	analytical	994:1003	arg1	SEM					1028:1030	SEM	1028:1030	SEM with EDAX	1028:1040	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	66	theme	analytical	994:1003	arg1	techniques					1005:1014	various spectro analytical techniques	978:1014	various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis	978:1067	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	1	67	theme	double	257:262	arg1	CS-LDHCs					292:299	CS-LDHCs	292:299	CS-LDHCs	292:299	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	1	67	theme	double	257:262	arg1	composite					281:289	chitosan/magnesium-aluminium layered double hydroxide hybrid composite	220:289	chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs)	220:300	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	9	68	theme	oil	1571:1573	arg1	technology					1583:1592	oil removal technology	1571:1592	oil removal technology	1571:1592	This work provides an apparent proposal for the growth of oil removal technology.
28126456	7	69	theme	adsorption	1270:1279	arg1	process					1281:1287	adsorption process	1270:1287	adsorption process	1270:1287	The mechanism of adsorption process was demonstrated by calculating ΔG°, ΔH° and ΔS° values from thermodynamic parameters in order to understand the nature of sorption process.
28126456	1	70	theme	hydroxide	264:272	arg1	CS-LDHCs					292:299	CS-LDHCs	292:299	CS-LDHCs	292:299	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	1	70	theme	hydroxide	264:272	arg1	composite					281:289	chitosan/magnesium-aluminium layered double hydroxide hybrid composite	220:289	chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs)	220:300	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	6	71	dep	Freundlich	1181:1190	arg1	models					1245:1250	isotherm models	1236:1250	isotherm models	1236:1250	To find out the best fit for the sorption process, the obtained adsorption equilibrium data was explained with Freundlich, Langmuir, Dubinin-Radushkevich and Tempkin isotherm models.
28126456	7	72	theme	thermodynamic	1350:1362	arg1	parameters					1364:1373	thermodynamic parameters	1350:1373	thermodynamic parameters	1350:1373	The mechanism of adsorption process was demonstrated by calculating ΔG°, ΔH° and ΔS° values from thermodynamic parameters in order to understand the nature of sorption process.
28126456	6	73	theme	sorption	1103:1110	arg1	process					1112:1118	the sorption process	1099:1118	the sorption process	1099:1118	To find out the best fit for the sorption process, the obtained adsorption equilibrium data was explained with Freundlich, Langmuir, Dubinin-Radushkevich and Tempkin isotherm models.
28126456	1	74	theme	hybrid	274:279	arg1	CS-LDHCs					292:299	CS-LDHCs	292:299	CS-LDHCs	292:299	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	1	74	theme	hybrid	274:279	arg1	composite					281:289	chitosan/magnesium-aluminium layered double hydroxide hybrid composite	220:289	chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs)	220:300	The recovery of oil from oil-in-water emulsion has been investigated using chitosan/magnesium-aluminium layered double hydroxide hybrid composite (CS-LDHCs) by a single co-precipitation method.
28126456	0	75	theme	composite	74:82	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of chitosan/Mg-Al layered double hydroxide composite for the removal of oil particles from oil-in-water emulsion.
28126456	0	75	theme	composite	74:82	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of chitosan/Mg-Al layered double hydroxide composite for the removal of oil particles from oil-in-water emulsion.
28126456	3	76	theme	effective	719:727	arg1	material					705:712	the material	701:712	the material more effective	701:727	The enhancement of adsorption properties by CS-LDHCs material were attributed to the high content of LDH in chitosan, which makes the material more effective towards immobilization of oily particles.
28126456	4	77	theme	physicochemical	835:849	arg1	pH					876:877	pH	876:877	pH	876:877	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	4	77	theme	physicochemical	835:849	arg1	parameters					851:860	different physicochemical parameters	825:860	different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature	825:926	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	4	77	theme	physicochemical	835:849	arg1	dose					880:883	dose	880:883	dose	880:883	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	4	77	theme	physicochemical	835:849	arg1	temperature					916:926	temperature	916:926	temperature	916:926	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	4	77	theme	physicochemical	835:849	arg1	time					870:873	time	870:873	time	870:873	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	4	77	theme	physicochemical	835:849	arg1	concentration					898:910	initial oil concentration	886:910	initial oil concentration	886:910	Batch experiment study has been elucidated by varying different physicochemical parameters such as time, pH, dose, initial oil concentration and temperature.
28126456	5	78	dep	techniques	1005:1014	arg1	analysis					1060:1067	DSC analysis	1056:1067	DSC analysis	1056:1067	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	78	dep	techniques	1005:1014	arg1	viz.					1016:1019	viz.	1016:1019	viz.	1016:1019	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	78	dep	techniques	1005:1014	arg1	FTIR					1022:1025	FTIR	1022:1025	FTIR	1022:1025	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	78	dep	techniques	1005:1014	arg1	XRD					1043:1045	XRD	1043:1045	XRD	1043:1045	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	78	dep	techniques	1005:1014	arg1	TGA					1048:1050	TGA	1048:1050	TGA	1048:1050	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	78	dep	techniques	1005:1014	arg1	SEM					1028:1030	SEM	1028:1030	SEM with EDAX	1028:1040	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	5	78	dep	techniques	1005:1014	arg1	techniques					1005:1014	various spectro analytical techniques	978:1014	various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis	978:1067	The as-synthesized CS-LDHCs was characterized by various spectro analytical techniques viz., FTIR, SEM with EDAX, XRD, TGA and DSC analysis.
28126456	7	79	theme	sorption	1412:1419	arg1	process					1421:1427	sorption process	1412:1427	sorption process	1412:1427	The mechanism of adsorption process was demonstrated by calculating ΔG°, ΔH° and ΔS° values from thermodynamic parameters in order to understand the nature of sorption process.
26968925	5	0	theme	poly	715:718	arg1	composite					728:736	The resultant poly(MAA-HS) composite	701:736	The resultant poly(MAA-HS) composite	701:736	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	1	1	theme	perborate	299:307	arg1	system					346:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	4	2	theme	MAA	665:667	arg1	percent					686:692	the MAA total conversion percent	661:692	the MAA total conversion percent (TC%)	661:698	The polymerization reaction was scrutinized through calculation of the MAA total conversion percent (TC%).
26968925	4	2	theme	MAA	665:667	arg1	%					697:697	TC%	695:697	TC%	695:697	The polymerization reaction was scrutinized through calculation of the MAA total conversion percent (TC%).
26968925	5	3	theme	HP	856:857	arg1	%					858:858	HP%	856:858	HP%	856:858	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	5	4	theme	grafting	813:820	arg1	efficiency					822:831	the grafting efficiency	809:831	the grafting efficiency	809:831	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	1	5	theme	methacrylic	232:242	arg1	MAA					250:252	MAA	250:252	MAA	250:252	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	1	5	theme	methacrylic	232:242	arg1	acid					244:247	methacrylic acid	232:247	methacrylic acid (MAA)	232:253	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	1	6	theme	SPB	310:312	arg1	system					346:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	3	7	theme	MAA	515:517	arg1	concentrations					497:510	the concentrations	493:510	the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature	493:591	These parameters comprehended the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature.
26968925	5	8	theme	total	869:873	arg1	conversion					875:884	the total conversion	865:884	the total conversion	865:884	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	1	9	theme	acid	244:247	arg1	polymerization					187:200	graft polymerization	181:200	graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA)	181:253	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	1	10	dep	starch-g-poly	124:136	arg1	acid					150:153	methacrylic acid	138:153	methacrylic acid	138:153	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	4	11	theme	percent	686:692	arg1	calculation					646:656	calculation	646:656	calculation of the MAA total conversion percent (TC%)	646:698	The polymerization reaction was scrutinized through calculation of the MAA total conversion percent (TC%).
26968925	1	12	theme	-thiourea	314:322	arg1	system					346:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	1	13	theme	composite	156:164	arg1	synthesis					111:119	the synthesis	107:119	the synthesis of starch-g-poly(methacrylic acid) composite	107:164	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	5	14	theme	MAA-HS	720:725	arg1	composite					728:736	The resultant poly(MAA-HS) composite	701:736	The resultant poly(MAA-HS) composite	701:736	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	7	15	theme	Tentative	1042:1050	arg1	mechanisms					1052:1061	Tentative mechanisms	1042:1061	Tentative mechanisms	1042:1061	Tentative mechanisms, which depict all occasions that happen amid the entire course of the polymerization reaction, have been proffered.
26968925	1	16	theme	TU	325:326	arg1	system					346:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	2	17	dep	efficiency	398:407	arg1	method					439:444	the polymerization method	420:444	the polymerization method	420:444	The parameters influencing the redox system efficiency and thence the polymerization method were considered.
26968925	7	18	theme	entire	1112:1117	arg1	course					1119:1124	the entire course	1108:1124	the entire course of the polymerization reaction	1108:1155	Tentative mechanisms, which depict all occasions that happen amid the entire course of the polymerization reaction, have been proffered.
26968925	3	19	theme	TU	525:526	arg1	concentrations					497:510	the concentrations	493:510	the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature	493:591	These parameters comprehended the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature.
26968925	6	20	theme	cooked	937:942	arg1	paste					971:975	the cooked poly(MAA)-starch composite paste	933:975	the cooked poly(MAA)-starch composite paste	933:975	The comportment of the apparent viscosity of the cooked poly(MAA)-starch composite paste, obtained under diverse polymerization conditions, was examined.
26968925	2	21	theme	system	391:396	arg1	efficiency					398:407	the redox system efficiency	381:407	the redox system efficiency	381:407	The parameters influencing the redox system efficiency and thence the polymerization method were considered.
26968925	6	22	theme	apparent	911:918	arg1	viscosity					920:928	the apparent viscosity	907:928	the apparent viscosity of the cooked poly(MAA)-starch composite paste	907:975	The comportment of the apparent viscosity of the cooked poly(MAA)-starch composite paste, obtained under diverse polymerization conditions, was examined.
26968925	0	23	dep	composite	77:85	arg1	acid					71:74	methacrylic acid	59:74	methacrylic acid	59:74	Synthesis and characterization of hydrolysed starch-g-poly(methacrylic acid) composite.
26968925	5	24	theme	GY	804:805	arg1	%					806:806	GY%	804:806	GY%	804:806	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	2	25	theme	redox	385:389	arg1	efficiency					398:407	the redox system efficiency	381:407	the redox system efficiency	381:407	The parameters influencing the redox system efficiency and thence the polymerization method were considered.
26968925	5	26	dep	yield	797:801	arg1	conversion					875:884	the total conversion	865:884	the total conversion	865:884	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	5	26	dep	yield	797:801	arg1	%					836:836	GE%	834:836	GE%	834:836	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	5	26	dep	yield	797:801	arg1	%					858:858	HP%	856:858	HP%	856:858	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	5	26	dep	yield	797:801	arg1	%					806:806	GY%	804:806	GY%	804:806	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	5	26	dep	yield	797:801	arg1	homopolymer					843:853	the homopolymer	839:853	the homopolymer	839:853	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	5	26	dep	yield	797:801	arg1	efficiency					822:831	the grafting efficiency	809:831	the grafting efficiency	809:831	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	3	27	theme	SPB	520:522	arg1	concentrations					497:510	the concentrations	493:510	the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature	493:591	These parameters comprehended the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature.
26968925	1	28	theme	graft	181:185	arg1	polymerization					187:200	graft polymerization	181:200	graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA)	181:253	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	1	29	theme	novel	90:94	arg1	method					96:101	A novel method	88:101	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite	88:164	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	6	30	theme	diverse	993:999	arg1	conditions					1016:1025	diverse polymerization conditions	993:1025	diverse polymerization conditions	993:1025	The comportment of the apparent viscosity of the cooked poly(MAA)-starch composite paste, obtained under diverse polymerization conditions, was examined.
26968925	6	31	theme	viscosity	920:928	arg1	comportment					892:902	The comportment	888:902	The comportment	888:902	The comportment of the apparent viscosity of the cooked poly(MAA)-starch composite paste, obtained under diverse polymerization conditions, was examined.
26968925	2	32	theme	polymerization	424:437	arg1	method					439:444	the polymerization method	420:444	the polymerization method	420:444	The parameters influencing the redox system efficiency and thence the polymerization method were considered.
26968925	1	33	theme	aqueous	258:264	arg1	medium					266:271	aqueous medium	258:271	aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	258:351	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	1	34	theme	redox	329:333	arg1	system					346:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	3	35	theme	ratio	545:549	arg1	concentrations					497:510	the concentrations	493:510	the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature	493:591	These parameters comprehended the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature.
26968925	4	36	theme	polymerization	598:611	arg1	reaction					613:620	The polymerization reaction	594:620	The polymerization reaction	594:620	The polymerization reaction was scrutinized through calculation of the MAA total conversion percent (TC%).
26968925	5	37	theme	graft	791:795	arg1	yield					797:801	the graft yield	787:801	the graft yield	787:801	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	1	38	theme	initiation	335:344	arg1	system					346:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	1	39	theme	hydrolysed	205:214	arg1	HS					224:225	HS	224:225	HS	224:225	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	1	39	theme	hydrolysed	205:214	arg1	starch					216:221	hydrolysed starch	205:221	hydrolysed starch (HS)	205:226	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	0	40	theme	methacrylic	59:69	arg1	acid					71:74	methacrylic acid	59:74	methacrylic acid	59:74	Synthesis and characterization of hydrolysed starch-g-poly(methacrylic acid) composite.
26968925	3	41	theme	SPB/TU	532:537	arg1	ratio					545:549	SPB/TU molar ratio	532:549	SPB/TU molar ratio	532:549	These parameters comprehended the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature.
26968925	4	42	theme	conversion	675:684	arg1	percent					686:692	the MAA total conversion percent	661:692	the MAA total conversion percent (TC%)	661:698	The polymerization reaction was scrutinized through calculation of the MAA total conversion percent (TC%).
26968925	4	42	theme	conversion	675:684	arg1	%					697:697	TC%	695:697	TC%	695:697	The polymerization reaction was scrutinized through calculation of the MAA total conversion percent (TC%).
26968925	3	43	theme	molar	539:543	arg1	ratio					545:549	SPB/TU molar ratio	532:549	SPB/TU molar ratio	532:549	These parameters comprehended the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature.
26968925	7	44	theme	polymerization	1133:1146	arg1	reaction					1148:1155	the polymerization reaction	1129:1155	the polymerization reaction	1129:1155	Tentative mechanisms, which depict all occasions that happen amid the entire course of the polymerization reaction, have been proffered.
26968925	3	45	theme	temperature	581:591	arg1	concentrations					497:510	the concentrations	493:510	the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature	493:591	These parameters comprehended the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature.
26968925	1	46	theme	efficient	282:290	arg1	system					346:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	5	47	theme	GE	834:835	arg1	%					836:836	GE%	834:836	GE%	834:836	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	6	48	theme	polymerization	1001:1014	arg1	conditions					1016:1025	diverse polymerization conditions	993:1025	diverse polymerization conditions	993:1025	The comportment of the apparent viscosity of the cooked poly(MAA)-starch composite paste, obtained under diverse polymerization conditions, was examined.
26968925	1	49	theme	starch	216:221	arg1	polymerization					187:200	graft polymerization	181:200	graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA)	181:253	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	6	50	theme	paste	971:975	arg1	viscosity					920:928	the apparent viscosity	907:928	the apparent viscosity of the cooked poly(MAA)-starch composite paste	907:975	The comportment of the apparent viscosity of the cooked poly(MAA)-starch composite paste, obtained under diverse polymerization conditions, was examined.
26968925	7	51	theme	reaction	1148:1155	arg1	course					1119:1124	the entire course	1108:1124	the entire course of the polymerization reaction	1108:1155	Tentative mechanisms, which depict all occasions that happen amid the entire course of the polymerization reaction, have been proffered.
26968925	3	52	theme	polymerization	566:579	arg1	temperature					581:591	the polymerization temperature	562:591	the polymerization temperature	562:591	These parameters comprehended the concentrations of MAA, SPB, TU and SPB/TU molar ratio as well as the polymerization temperature.
26968925	1	53	theme	starch-g-poly	124:136	arg1	composite					156:164	starch-g-poly(methacrylic acid) composite	124:164	starch-g-poly(methacrylic acid) composite	124:164	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	6	54	theme	-starch	953:959	arg1	paste					971:975	the cooked poly(MAA)-starch composite paste	933:975	the cooked poly(MAA)-starch composite paste	933:975	The comportment of the apparent viscosity of the cooked poly(MAA)-starch composite paste, obtained under diverse polymerization conditions, was examined.
26968925	5	55	theme	polymer	769:775	arg1	criteria					777:784	the polymer criteria	765:784	the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion)	765:885	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	6	56	theme	composite	961:969	arg1	paste					971:975	the cooked poly(MAA)-starch composite paste	933:975	the cooked poly(MAA)-starch composite paste	933:975	The comportment of the apparent viscosity of the cooked poly(MAA)-starch composite paste, obtained under diverse polymerization conditions, was examined.
26968925	5	57	theme	resultant	705:713	arg1	composite					728:736	The resultant poly(MAA-HS) composite	701:736	The resultant poly(MAA-HS) composite	701:736	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	1	58	theme	sodium	292:297	arg1	system					346:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system	279:351	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	5	59	dep	criteria	777:784	arg1	yield					797:801	the graft yield	787:801	the graft yield	787:801	The resultant poly(MAA-HS) composite was assessed by evaluating the polymer criteria (the graft yield, GY%; the grafting efficiency, GE%; the homopolymer, HP%; and the total conversion).
26968925	1	60	theme	methacrylic	138:148	arg1	acid					150:153	methacrylic acid	138:153	methacrylic acid	138:153	A novel method for the synthesis of starch-g-poly(methacrylic acid) composite was adopted by graft polymerization of hydrolysed starch (HS) and methacrylic acid (MAA) in aqueous medium using an efficient sodium perborate (SPB)-thiourea (TU) redox initiation system.
26968925	4	61	theme	total	669:673	arg1	percent					686:692	the MAA total conversion percent	661:692	the MAA total conversion percent (TC%)	661:698	The polymerization reaction was scrutinized through calculation of the MAA total conversion percent (TC%).
26968925	4	61	theme	total	669:673	arg1	%					697:697	TC%	695:697	TC%	695:697	The polymerization reaction was scrutinized through calculation of the MAA total conversion percent (TC%).
26840728	7	0	theme	bilayer	1220:1226	arg1	models					1235:1240	bilayer domain models	1220:1240	bilayer domain models	1220:1240	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	2	1	theme	lipid	376:380	arg1	bilayer					382:388	a supported lipid bilayer	364:388	a supported lipid bilayer as a function of cholesterol mole fraction	364:431	Here we measured the rate at which methyl-β-cyclodextrin depletes cholesterol from a supported lipid bilayer as a function of cholesterol mole fraction.
26840728	6	2	theme	membrane	997:1004	arg1	cholesterol					1006:1016	the total membrane cholesterol	987:1016	the total membrane cholesterol	987:1016	A larger fraction of the total membrane cholesterol appears in the more accessible population when the acyl chains of the PC-lipid tails are more unsaturated.
26840728	0	3	theme	Lipid	76:80	arg1	Membranes					82:90	Model Lipid Membranes	70:90	Model Lipid Membranes	70:90	Depletion with Cyclodextrin Reveals Two Populations of Cholesterol in Model Lipid Membranes.
26840728	2	4	theme	supported	366:374	arg1	bilayer					382:388	a supported lipid bilayer	364:388	a supported lipid bilayer as a function of cholesterol mole fraction	364:431	Here we measured the rate at which methyl-β-cyclodextrin depletes cholesterol from a supported lipid bilayer as a function of cholesterol mole fraction.
26840728	1	5	theme	membrane	259:266	arg1	composition					268:278	membrane composition	259:278	membrane composition	259:278	Recent results provide evidence that cholesterol is highly accessible for removal from both cell and model membranes above a threshold concentration that varies with membrane composition.
26840728	6	6	theme	PC-lipid	1088:1095	arg1	tails					1097:1101	the PC-lipid tails	1084:1101	the PC-lipid tails	1084:1101	A larger fraction of the total membrane cholesterol appears in the more accessible population when the acyl chains of the PC-lipid tails are more unsaturated.
26840728	7	7	from	interactions	1270:1281	arg1	membranes					1292:1300	lipid membranes	1286:1300	lipid membranes	1286:1300	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	6	8	theme	accessible	1038:1047	arg1	population					1049:1058	the more accessible population	1029:1058	the more accessible population	1029:1058	A larger fraction of the total membrane cholesterol appears in the more accessible population when the acyl chains of the PC-lipid tails are more unsaturated.
26840728	7	9	theme	cholesterol	1208:1218	arg1	predictions					1167:1177	the predictions	1163:1177	the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes	1163:1300	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	7	10	from	condensed-complex	1186:1202	arg1	membranes					1292:1300	lipid membranes	1286:1300	lipid membranes	1286:1300	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	4	11	theme	individual	759:768	arg1	bilayers					770:777	many individual bilayers	754:777	many individual bilayers	754:777	Our technique yields the accessibility of cholesterol over a wide range of concentrations (30-66 mol %) for many individual bilayers, enabling fast acquisition of replicate data.
26840728	7	12	theme	interactions	1270:1281	arg1	cholesterol					1208:1218	cholesterol	1208:1218	cholesterol	1208:1218	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	7	12	theme	interactions	1270:1281	arg1	condensed-complex					1186:1202	condensed-complex	1186:1202	condensed-complex	1186:1202	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	3	13	theme	phosphatidylcholine	516:534	arg1	mixtures					482:489	two-component mixtures	468:489	two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid	468:541	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	4	14	theme	concentrations	721:734	arg1	range					712:716	a wide range	705:716	a wide range of concentrations (30-66 mol %) for many individual bilayers	705:777	Our technique yields the accessibility of cholesterol over a wide range of concentrations (30-66 mol %) for many individual bilayers, enabling fast acquisition of replicate data.
26840728	4	15	theme	cholesterol	688:698	arg1	accessibility					671:683	the accessibility	667:683	the accessibility of cholesterol	667:698	Our technique yields the accessibility of cholesterol over a wide range of concentrations (30-66 mol %) for many individual bilayers, enabling fast acquisition of replicate data.
26840728	2	16	theme	cholesterol	407:417	arg1	fraction					424:431	cholesterol mole fraction	407:431	cholesterol mole fraction	407:431	Here we measured the rate at which methyl-β-cyclodextrin depletes cholesterol from a supported lipid bilayer as a function of cholesterol mole fraction.
26840728	7	17	theme	condensed-complex	1186:1202	arg1	predictions					1167:1177	the predictions	1163:1177	the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes	1163:1300	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	5	18	theme	low	901:903	arg1	accessibility					913:925	low surface accessibility	901:925	low surface accessibility	901:925	We found that the bilayers contain two populations of cholesterol, one with low surface accessibility and the other with high accessibility.
26840728	5	19	contain	contain	852:858	arg1	bilayers					843:850	the bilayers	839:850	the bilayers	839:850	We found that the bilayers contain two populations of cholesterol, one with low surface accessibility and the other with high accessibility.
26840728	5	19	contain	contain	852:858	arg2	other					935:939	other	935:939	other	935:939	We found that the bilayers contain two populations of cholesterol, one with low surface accessibility and the other with high accessibility.
26840728	5	19	contain	contain	852:858	arg2	populations					864:874	two populations	860:874	two populations of cholesterol	860:889	We found that the bilayers contain two populations of cholesterol, one with low surface accessibility and the other with high accessibility.
26840728	5	19	contain	contain	852:858	arg2	one					892:894	one	892:894	one	892:894	We found that the bilayers contain two populations of cholesterol, one with low surface accessibility and the other with high accessibility.
26840728	7	20	with	consistent	1147:1156	arg1	predictions					1167:1177	the predictions	1163:1177	the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes	1163:1300	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	7	21	theme	domain	1228:1233	arg1	models					1235:1240	bilayer domain models	1220:1240	bilayer domain models	1220:1240	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	3	22	with	bilayers	607:614	arg1	microscopy					634:643	fluorescence microscopy	621:643	fluorescence microscopy	621:643	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	3	23	dep	phosphatidylcholine	516:534	arg1	lipid					537:541	lipid	537:541	a PC (phosphatidylcholine) lipid	510:541	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	1	24	theme	cell	185:188	arg1	membranes					200:208	both cell and model membranes	180:208	membranes	200:208	Recent results provide evidence that cholesterol is highly accessible for removal from both cell and model membranes above a threshold concentration that varies with membrane composition.
26840728	1	25	theme	Recent	93:98	arg1	results					100:106	Recent results	93:106	Recent results	93:106	Recent results provide evidence that cholesterol is highly accessible for removal from both cell and model membranes above a threshold concentration that varies with membrane composition.
26840728	3	26	theme	supported	444:452	arg1	bilayers					454:461	supported bilayers	444:461	supported bilayers	444:461	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	5	27	with	other	935:939	arg1	accessibility					951:963	high accessibility	946:963	high accessibility	946:963	We found that the bilayers contain two populations of cholesterol, one with low surface accessibility and the other with high accessibility.
26840728	6	28	theme	larger	968:973	arg1	fraction					975:982	A larger fraction	966:982	A larger fraction of the total membrane cholesterol	966:1016	A larger fraction of the total membrane cholesterol appears in the more accessible population when the acyl chains of the PC-lipid tails are more unsaturated.
26840728	2	29	from	bilayer	382:388	arg1	cholesterol					347:357	cholesterol	347:357	cholesterol from a supported lipid bilayer as a function of cholesterol mole fraction	347:431	Here we measured the rate at which methyl-β-cyclodextrin depletes cholesterol from a supported lipid bilayer as a function of cholesterol mole fraction.
26840728	4	30	theme	many	754:757	arg1	bilayers					770:777	many individual bilayers	754:777	many individual bilayers	754:777	Our technique yields the accessibility of cholesterol over a wide range of concentrations (30-66 mol %) for many individual bilayers, enabling fast acquisition of replicate data.
26840728	3	31	from	area	595:598	arg1	rate					575:578	the rate	571:578	the rate of decrease in area of the bilayers with fluorescence microscopy	571:643	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	1	32	theme	model	194:198	arg1	membranes					200:208	both cell and model membranes	180:208	membranes	200:208	Recent results provide evidence that cholesterol is highly accessible for removal from both cell and model membranes above a threshold concentration that varies with membrane composition.
26840728	3	33	theme	decrease	583:590	arg1	rate					575:578	the rate	571:578	the rate of decrease in area of the bilayers with fluorescence microscopy	571:643	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	4	34	theme	wide	707:710	arg1	range					712:716	a wide range	705:716	a wide range of concentrations (30-66 mol %) for many individual bilayers	705:777	Our technique yields the accessibility of cholesterol over a wide range of concentrations (30-66 mol %) for many individual bilayers, enabling fast acquisition of replicate data.
26840728	3	35	theme	cholesterol	494:504	arg1	mixtures					482:489	two-component mixtures	468:489	two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid	468:541	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	3	36	theme	two-component	468:480	arg1	mixtures					482:489	two-component mixtures	468:489	two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid	468:541	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	5	37	theme	cholesterol	879:889	arg1	populations					864:874	two populations	860:874	two populations of cholesterol	860:889	We found that the bilayers contain two populations of cholesterol, one with low surface accessibility and the other with high accessibility.
26840728	5	37	theme	cholesterol	879:889	arg1	other					935:939	other	935:939	other	935:939	We found that the bilayers contain two populations of cholesterol, one with low surface accessibility and the other with high accessibility.
26840728	5	37	theme	cholesterol	879:889	arg1	one					892:894	one	892:894	one	892:894	We found that the bilayers contain two populations of cholesterol, one with low surface accessibility and the other with high accessibility.
26840728	2	38	theme	fraction	424:431	arg1	function					395:402	a function	393:402	a function of cholesterol mole fraction	393:431	Here we measured the rate at which methyl-β-cyclodextrin depletes cholesterol from a supported lipid bilayer as a function of cholesterol mole fraction.
26840728	6	39	theme	acyl	1069:1072	arg1	unsaturated					1112:1122	unsaturated	1112:1122	unsaturated	1112:1122	A larger fraction of the total membrane cholesterol appears in the more accessible population when the acyl chains of the PC-lipid tails are more unsaturated.
26840728	6	39	theme	acyl	1069:1072	arg1	chains					1074:1079	the acyl chains	1065:1079	the acyl chains of the PC-lipid tails	1065:1101	A larger fraction of the total membrane cholesterol appears in the more accessible population when the acyl chains of the PC-lipid tails are more unsaturated.
26840728	6	40	theme	total	991:995	arg1	cholesterol					1006:1016	the total membrane cholesterol	987:1016	the total membrane cholesterol	987:1016	A larger fraction of the total membrane cholesterol appears in the more accessible population when the acyl chains of the PC-lipid tails are more unsaturated.
26840728	4	41	theme	data	819:822	arg1	acquisition					794:804	fast acquisition	789:804	fast acquisition of replicate data	789:822	Our technique yields the accessibility of cholesterol over a wide range of concentrations (30-66 mol %) for many individual bilayers, enabling fast acquisition of replicate data.
26840728	2	42	theme	mole	419:422	arg1	fraction					424:431	cholesterol mole fraction	407:431	cholesterol mole fraction	407:431	Here we measured the rate at which methyl-β-cyclodextrin depletes cholesterol from a supported lipid bilayer as a function of cholesterol mole fraction.
26840728	0	43	theme	Cholesterol	55:65	arg1	Populations					40:50	Two Populations	36:50	Two Populations of Cholesterol	36:65	Depletion with Cyclodextrin Reveals Two Populations of Cholesterol in Model Lipid Membranes.
26840728	7	44	from	cholesterol	1208:1218	arg1	membranes					1292:1300	lipid membranes	1286:1300	lipid membranes	1286:1300	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	3	45	theme	PC	512:513	arg1	phosphatidylcholine					516:534	a PC (phosphatidylcholine)	510:535	a PC (phosphatidylcholine) lipid	510:541	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	5	46	theme	high	946:949	arg1	accessibility					951:963	high accessibility	946:963	high accessibility	946:963	We found that the bilayers contain two populations of cholesterol, one with low surface accessibility and the other with high accessibility.
26840728	7	47	dep	condensed-complex	1186:1202	arg1	the					1182:1184	the	1182:1184	the	1182:1184	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	7	47	dep	condensed-complex	1186:1202	arg1	models					1235:1240	bilayer domain models	1220:1240	bilayer domain models	1220:1240	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	7	48	from	membranes	1292:1300	arg1	cholesterol					1208:1218	cholesterol	1208:1218	cholesterol	1208:1218	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	7	48	from	membranes	1292:1300	arg1	condensed-complex					1186:1202	condensed-complex	1186:1202	condensed-complex	1186:1202	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	3	49	theme	fluorescence	621:632	arg1	microscopy					634:643	fluorescence microscopy	621:643	fluorescence microscopy	621:643	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	1	50	from	membranes	200:208	arg1	removal					167:173	removal	167:173	removal from both cell and model membranes above a threshold concentration that varies with membrane composition	167:278	Recent results provide evidence that cholesterol is highly accessible for removal from both cell and model membranes above a threshold concentration that varies with membrane composition.
26840728	4	51	theme	30-66 mol	737:745	arg1	concentrations					721:734	concentrations	721:734	concentrations (30-66 mol %)	721:748	Our technique yields the accessibility of cholesterol over a wide range of concentrations (30-66 mol %) for many individual bilayers, enabling fast acquisition of replicate data.
26840728	4	51	theme	30-66 mol	737:745	arg1	%					747:747	30-66 mol %	737:747	30-66 mol %	737:747	Our technique yields the accessibility of cholesterol over a wide range of concentrations (30-66 mol %) for many individual bilayers, enabling fast acquisition of replicate data.
26840728	3	52	from	rate	575:578	arg1	area					595:598	area	595:598	area of the bilayers with fluorescence microscopy	595:643	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	1	53	theme	threshold	218:226	arg1	concentration					228:240	a threshold concentration	216:240	a threshold concentration that varies with membrane composition	216:278	Recent results provide evidence that cholesterol is highly accessible for removal from both cell and model membranes above a threshold concentration that varies with membrane composition.
26840728	0	54	with	Depletion	0:8	arg1	Cyclodextrin					15:26	Cyclodextrin	15:26	Cyclodextrin	15:26	Depletion with Cyclodextrin Reveals Two Populations of Cholesterol in Model Lipid Membranes.
26840728	5	55	theme	surface	905:911	arg1	accessibility					913:925	low surface accessibility	901:925	low surface accessibility	901:925	We found that the bilayers contain two populations of cholesterol, one with low surface accessibility and the other with high accessibility.
26840728	4	56	theme	fast	789:792	arg1	acquisition					794:804	fast acquisition	789:804	fast acquisition of replicate data	789:822	Our technique yields the accessibility of cholesterol over a wide range of concentrations (30-66 mol %) for many individual bilayers, enabling fast acquisition of replicate data.
26840728	7	57	theme	lipid	1286:1290	arg1	membranes					1292:1300	lipid membranes	1286:1300	lipid membranes	1286:1300	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	0	58	theme	Model	70:74	arg1	Membranes					82:90	Model Lipid Membranes	70:90	Model Lipid Membranes	70:90	Depletion with Cyclodextrin Reveals Two Populations of Cholesterol in Model Lipid Membranes.
26840728	4	59	dep	data	819:822	arg1	replicate					809:817	replicate	809:817	replicate	809:817	Our technique yields the accessibility of cholesterol over a wide range of concentrations (30-66 mol %) for many individual bilayers, enabling fast acquisition of replicate data.
26840728	3	60	theme	bilayers	607:614	arg1	area					595:598	area	595:598	area of the bilayers with fluorescence microscopy	595:643	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	3	61	from	decrease	583:590	arg1	area					595:598	area	595:598	area of the bilayers with fluorescence microscopy	595:643	We formed supported bilayers from two-component mixtures of cholesterol and a PC (phosphatidylcholine) lipid, and we directly visualized the rate of decrease in area of the bilayers with fluorescence microscopy.
26840728	6	62	theme	tails	1097:1101	arg1	unsaturated					1112:1122	unsaturated	1112:1122	unsaturated	1112:1122	A larger fraction of the total membrane cholesterol appears in the more accessible population when the acyl chains of the PC-lipid tails are more unsaturated.
26840728	6	62	theme	tails	1097:1101	arg1	chains					1074:1079	the acyl chains	1065:1079	the acyl chains of the PC-lipid tails	1065:1101	A larger fraction of the total membrane cholesterol appears in the more accessible population when the acyl chains of the PC-lipid tails are more unsaturated.
26840728	7	63	theme	cholesterol-phospholipid	1245:1268	arg1	interactions					1270:1281	cholesterol-phospholipid interactions	1245:1281	cholesterol-phospholipid interactions in lipid membranes	1245:1300	Our findings are most consistent with the predictions of the condensed-complex and cholesterol bilayer domain models of cholesterol-phospholipid interactions in lipid membranes.
26840728	6	64	theme	cholesterol	1006:1016	arg1	fraction					975:982	A larger fraction	966:982	A larger fraction of the total membrane cholesterol	966:1016	A larger fraction of the total membrane cholesterol appears in the more accessible population when the acyl chains of the PC-lipid tails are more unsaturated.
26053617	8	0	theme	antibiotics	1335:1345	arg1	effects					1324:1330	the adverse effects	1312:1330	the adverse effects of antibiotics	1312:1345	There is therefore potential for using supplements to lessen the adverse effects of antibiotics.
26053617	1	1	theme	hormone	307:313	arg1	regulation					315:324	gut hormone regulation	303:324	gut hormone regulation	303:324	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	9	2	theme	therapeutic	1406:1416	arg1	manipulation					1418:1429	better therapeutic manipulation	1399:1429	better therapeutic manipulation of the human gut microbiota	1399:1457	Further knowledge of such mechanisms could lead to better therapeutic manipulation of the human gut microbiota.
26053617	9	3	theme	mechanisms	1374:1383	arg1	knowledge					1356:1364	Further knowledge	1348:1364	Further knowledge of such mechanisms	1348:1383	Further knowledge of such mechanisms could lead to better therapeutic manipulation of the human gut microbiota.
26053617	1	4	contain	carry	120:124	arg1	bacteria					111:118	Intestinal bacteria	100:118	Intestinal bacteria	100:118	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	1	4	contain	carry	120:124	arg2	roles					147:151	many fundamental roles	130:151	many fundamental roles	130:151	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	1	4	contain	carry	120:124	arg2	fermentation					166:177	the fermentation	162:177	the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs)	162:260	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	4	5	used	used	710:713	arg2	method					647:652	dietary supplementation--another method	614:652	dietary supplementation--another method	614:652	Here, we have investigated whether dietary supplementation--another method used to modulate gut composition and function--could be used to ameliorate the side effects of antibiotics.
26053617	5	6	theme	bacterial	779:787	arg1	communities					789:799	gut bacterial communities	775:799	gut bacterial communities	775:799	We perturbed gut bacterial communities with gentamicin and ampicillin in anaerobic batch cultures in vitro.
26053617	6	7	dep	pectin	909:914	arg1	fibre					935:939	fibre	935:939	fibre	935:939	Cultures were supplemented with either pectin (a non-fermentable fibre), inulin (a commonly used prebiotic that promotes the growth of beneficial bacteria) or neither.
26053617	7	8	theme	dietary	1099:1105	arg1	supplementation					1107:1121	dietary supplementation	1099:1121	dietary supplementation	1099:1121	Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics.
26053617	4	9	theme	antibiotics	749:759	arg1	effects					738:744	the side effects	729:744	the side effects of antibiotics	729:759	Here, we have investigated whether dietary supplementation--another method used to modulate gut composition and function--could be used to ameliorate the side effects of antibiotics.
26053617	4	10	theme	side	733:736	arg1	effects					738:744	the side effects	729:744	the side effects of antibiotics	729:759	Here, we have investigated whether dietary supplementation--another method used to modulate gut composition and function--could be used to ameliorate the side effects of antibiotics.
26053617	9	11	theme	gut	1444:1446	arg1	microbiota					1448:1457	the human gut microbiota	1434:1457	the human gut microbiota	1434:1457	Further knowledge of such mechanisms could lead to better therapeutic manipulation of the human gut microbiota.
26053617	7	12	theme	supplementation	1107:1121	arg1	effects					1088:1094	the beneficial effects	1073:1094	the beneficial effects of dietary supplementation	1073:1121	Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics.
26053617	9	13	theme	microbiota	1448:1457	arg1	manipulation					1418:1429	better therapeutic manipulation	1399:1429	better therapeutic manipulation of the human gut microbiota	1399:1457	Further knowledge of such mechanisms could lead to better therapeutic manipulation of the human gut microbiota.
26053617	5	14	from	ampicillin	821:830	arg1	cultures					851:858	anaerobic batch cultures	835:858	anaerobic batch cultures	835:858	We perturbed gut bacterial communities with gentamicin and ampicillin in anaerobic batch cultures in vitro.
26053617	5	15	theme	anaerobic	835:843	arg1	cultures					851:858	anaerobic batch cultures	835:858	anaerobic batch cultures	835:858	We perturbed gut bacterial communities with gentamicin and ampicillin in anaerobic batch cultures in vitro.
26053617	7	16	theme	dietary	1187:1193	arg1	supplementation					1195:1209	dietary supplementation	1187:1209	dietary supplementation	1187:1209	Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics.
26053617	0	17	dep	in	90:91	arg1	Vitro					93:97	Vitro	93:97	Vitro	93:97	Prebiotics Modulate the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro.
26053617	4	18	theme	supplementation--another	622:645	arg1	method					647:652	dietary supplementation--another method	614:652	dietary supplementation--another method	614:652	Here, we have investigated whether dietary supplementation--another method used to modulate gut composition and function--could be used to ameliorate the side effects of antibiotics.
26053617	5	19	from	gentamicin	806:815	arg1	cultures					851:858	anaerobic batch cultures	835:858	anaerobic batch cultures	835:858	We perturbed gut bacterial communities with gentamicin and ampicillin in anaerobic batch cultures in vitro.
26053617	7	20	dep	combinations	1142:1153	arg1	combinations					1142:1153	some treatment combinations	1127:1153	some treatment combinations	1127:1153	Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics.
26053617	7	20	dep	combinations	1142:1153	arg1	ampicillin					1164:1173	ampicillin	1164:1173	ampicillin	1164:1173	Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics.
26053617	7	20	dep	combinations	1142:1153	arg1	inulin					1179:1184	inulin	1179:1184	inulin	1179:1184	Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics.
26053617	9	21	theme	Further	1348:1354	arg1	knowledge					1356:1364	Further knowledge	1348:1364	Further knowledge of such mechanisms	1348:1383	Further knowledge of such mechanisms could lead to better therapeutic manipulation of the human gut microbiota.
26053617	2	22	theme	challenging	414:424	arg1	goal					426:429	an important but challenging goal	397:429	an important but challenging goal	397:429	Understanding how to manage this ecosystem to improve human health is an important but challenging goal.
26053617	2	22	theme	challenging	414:424	arg1	Understanding					327:339	Understanding	327:339	Understanding how to manage this ecosystem to improve human health	327:392	Understanding how to manage this ecosystem to improve human health is an important but challenging goal.
26053617	6	23	theme	used	962:965	arg1	prebiotic					967:975	a commonly used prebiotic	951:975	a commonly used prebiotic that promotes the growth of beneficial bacteria	951:1023	Cultures were supplemented with either pectin (a non-fermentable fibre), inulin (a commonly used prebiotic that promotes the growth of beneficial bacteria) or neither.
26053617	7	24	theme	beneficial	1077:1086	arg1	effects					1088:1094	the beneficial effects	1073:1094	the beneficial effects of dietary supplementation	1073:1121	Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics.
26053617	3	25	theme	indigenous	534:543	arg1	diversity					555:563	indigenous microbial diversity	534:563	indigenous microbial diversity	534:563	Antibiotics are the front line of defence against pathogens, but in turn they have adverse effects on indigenous microbial diversity and function.
26053617	5	26	theme	batch	845:849	arg1	cultures					851:858	anaerobic batch cultures	835:858	anaerobic batch cultures	835:858	We perturbed gut bacterial communities with gentamicin and ampicillin in anaerobic batch cultures in vitro.
26053617	8	27	theme	adverse	1316:1322	arg1	effects					1324:1330	the adverse effects	1312:1330	the adverse effects of antibiotics	1312:1345	There is therefore potential for using supplements to lessen the adverse effects of antibiotics.
26053617	3	28	theme	adverse	515:521	arg1	effects					523:529	adverse effects	515:529	adverse effects	515:529	Antibiotics are the front line of defence against pathogens, but in turn they have adverse effects on indigenous microbial diversity and function.
26053617	1	29	theme	non-digestible	182:195	arg1	carbohydrates					205:217	non-digestible dietary carbohydrates	182:217	non-digestible dietary carbohydrates	182:217	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	2	30	theme	important	400:408	arg1	goal					426:429	an important but challenging goal	397:429	an important but challenging goal	397:429	Understanding how to manage this ecosystem to improve human health is an important but challenging goal.
26053617	2	30	theme	important	400:408	arg1	Understanding					327:339	Understanding	327:339	Understanding how to manage this ecosystem to improve human health	327:392	Understanding how to manage this ecosystem to improve human health is an important but challenging goal.
26053617	1	31	theme	Intestinal	100:109	arg1	bacteria					111:118	Intestinal bacteria	100:118	Intestinal bacteria	100:118	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	1	32	theme	dietary	197:203	arg1	carbohydrates					205:217	non-digestible dietary carbohydrates	182:217	non-digestible dietary carbohydrates	182:217	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	0	33	theme	Antibiotics	35:45	arg1	Effects					24:30	the Effects	20:30	the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro	20:97	Prebiotics Modulate the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro.
26053617	9	34	theme	such	1369:1372	arg1	mechanisms					1374:1383	such mechanisms	1369:1383	such mechanisms	1369:1383	Further knowledge of such mechanisms could lead to better therapeutic manipulation of the human gut microbiota.
26053617	4	35	theme	dietary	614:620	arg1	method					647:652	dietary supplementation--another method	614:652	dietary supplementation--another method	614:652	Here, we have investigated whether dietary supplementation--another method used to modulate gut composition and function--could be used to ameliorate the side effects of antibiotics.
26053617	1	36	theme	carbohydrates	205:217	arg1	fermentation					166:177	the fermentation	162:177	the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs)	162:260	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	4	37	theme	gut	671:673	arg1	composition					675:685	gut composition	671:685	gut composition	671:685	Here, we have investigated whether dietary supplementation--another method used to modulate gut composition and function--could be used to ameliorate the side effects of antibiotics.
26053617	1	38	theme	host	280:283	arg1	levels					292:297	host energy levels	280:297	host energy levels	280:297	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	5	39	theme	gut	775:777	arg1	communities					789:799	gut bacterial communities	775:799	gut bacterial communities	775:799	We perturbed gut bacterial communities with gentamicin and ampicillin in anaerobic batch cultures in vitro.
26053617	6	40	dep	inulin	943:948	arg1	prebiotic					967:975	a commonly used prebiotic	951:975	a commonly used prebiotic that promotes the growth of beneficial bacteria	951:1023	Cultures were supplemented with either pectin (a non-fermentable fibre), inulin (a commonly used prebiotic that promotes the growth of beneficial bacteria) or neither.
26053617	4	41	used	used	654:657	arg2	method					647:652	dietary supplementation--another method	614:652	dietary supplementation--another method	614:652	Here, we have investigated whether dietary supplementation--another method used to modulate gut composition and function--could be used to ameliorate the side effects of antibiotics.
26053617	2	42	theme	human	381:385	arg1	health					387:392	human health	381:392	human health	381:392	Understanding how to manage this ecosystem to improve human health is an important but challenging goal.
26053617	0	43	theme	Gut	50:52	arg1	Diversity					64:72	Gut Microbial Diversity	50:72	Gut Microbial Diversity	50:72	Prebiotics Modulate the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro.
26053617	1	44	theme	energy	285:290	arg1	levels					292:297	host energy levels	280:297	host energy levels	280:297	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	7	45	theme	antibiotics	1238:1248	arg1	effects					1227:1233	the effects	1223:1233	the effects of antibiotics	1223:1248	Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics.
26053617	3	46	theme	microbial	545:553	arg1	diversity					555:563	indigenous microbial diversity	534:563	indigenous microbial diversity	534:563	Antibiotics are the front line of defence against pathogens, but in turn they have adverse effects on indigenous microbial diversity and function.
26053617	7	47	theme	treatment	1132:1140	arg1	combinations					1142:1153	some treatment combinations	1127:1153	some treatment combinations	1127:1153	Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics.
26053617	7	47	theme	treatment	1132:1140	arg1	ampicillin					1164:1173	ampicillin	1164:1173	ampicillin	1164:1173	Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics.
26053617	7	47	theme	treatment	1132:1140	arg1	inulin					1179:1184	inulin	1179:1184	inulin	1179:1184	Although antibiotics often negated the beneficial effects of dietary supplementation, in some treatment combinations, notably ampicillin and inulin, dietary supplementation ameliorated the effects of antibiotics.
26053617	3	48	contain	have	510:513	arg1	they					505:508	they	505:508	they	505:508	Antibiotics are the front line of defence against pathogens, but in turn they have adverse effects on indigenous microbial diversity and function.
26053617	3	48	contain	have	510:513	arg2	effects					523:529	adverse effects	515:529	adverse effects	515:529	Antibiotics are the front line of defence against pathogens, but in turn they have adverse effects on indigenous microbial diversity and function.
26053617	1	49	theme	gut	303:305	arg1	regulation					315:324	gut hormone regulation	303:324	gut hormone regulation	303:324	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	3	50	theme	defence	466:472	arg1	Antibiotics					432:442	Antibiotics	432:442	Antibiotics	432:442	Antibiotics are the front line of defence against pathogens, but in turn they have adverse effects on indigenous microbial diversity and function.
26053617	3	50	theme	defence	466:472	arg1	line					458:461	the front line	448:461	the front line of defence against pathogens	448:490	Antibiotics are the front line of defence against pathogens, but in turn they have adverse effects on indigenous microbial diversity and function.
26053617	6	51	theme	beneficial	1005:1014	arg1	bacteria					1016:1023	beneficial bacteria	1005:1023	beneficial bacteria	1005:1023	Cultures were supplemented with either pectin (a non-fermentable fibre), inulin (a commonly used prebiotic that promotes the growth of beneficial bacteria) or neither.
26053617	3	52	theme	front	452:456	arg1	Antibiotics					432:442	Antibiotics	432:442	Antibiotics	432:442	Antibiotics are the front line of defence against pathogens, but in turn they have adverse effects on indigenous microbial diversity and function.
26053617	3	52	theme	front	452:456	arg1	line					458:461	the front line	448:461	the front line of defence against pathogens	448:490	Antibiotics are the front line of defence against pathogens, but in turn they have adverse effects on indigenous microbial diversity and function.
26053617	6	53	theme	bacteria	1016:1023	arg1	growth					995:1000	the growth	991:1000	the growth of beneficial bacteria	991:1023	Cultures were supplemented with either pectin (a non-fermentable fibre), inulin (a commonly used prebiotic that promotes the growth of beneficial bacteria) or neither.
26053617	9	54	theme	human	1438:1442	arg1	microbiota					1448:1457	the human gut microbiota	1434:1457	the human gut microbiota	1434:1457	Further knowledge of such mechanisms could lead to better therapeutic manipulation of the human gut microbiota.
26053617	1	55	theme	many	130:133	arg1	fermentation					166:177	the fermentation	162:177	the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs)	162:260	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	1	55	theme	many	130:133	arg1	roles					147:151	many fundamental roles	130:151	many fundamental roles	130:151	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	1	56	theme	short	230:234	arg1	SCFAs					255:259	SCFAs	255:259	SCFAs	255:259	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	1	56	theme	short	230:234	arg1	acids					248:252	short chain fatty acids	230:252	short chain fatty acids (SCFAs)	230:260	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	1	57	theme	fundamental	135:145	arg1	fermentation					166:177	the fermentation	162:177	the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs)	162:260	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	1	57	theme	fundamental	135:145	arg1	roles					147:151	many fundamental roles	130:151	many fundamental roles	130:151	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	1	58	theme	chain	236:240	arg1	SCFAs					255:259	SCFAs	255:259	SCFAs	255:259	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	1	58	theme	chain	236:240	arg1	acids					248:252	short chain fatty acids	230:252	short chain fatty acids (SCFAs)	230:260	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	0	59	theme	Microbial	54:62	arg1	Diversity					64:72	Gut Microbial Diversity	50:72	Gut Microbial Diversity	50:72	Prebiotics Modulate the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro.
26053617	0	60	from	Effects	24:30	arg1	Functioning					78:88	Functioning	78:88	Functioning	78:88	Prebiotics Modulate the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro.
26053617	0	60	from	Effects	24:30	arg1	Diversity					64:72	Gut Microbial Diversity	50:72	Gut Microbial Diversity	50:72	Prebiotics Modulate the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro.
26053617	1	61	theme	fatty	242:246	arg1	SCFAs					255:259	SCFAs	255:259	SCFAs	255:259	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
26053617	1	61	theme	fatty	242:246	arg1	acids					248:252	short chain fatty acids	230:252	short chain fatty acids (SCFAs)	230:260	Intestinal bacteria carry out many fundamental roles, such as the fermentation of non-digestible dietary carbohydrates to produce short chain fatty acids (SCFAs), which can affect host energy levels and gut hormone regulation.
29352894	5	0	theme	good	682:685	arg1	activity					701:708	good antibacterial activity	682:708	good antibacterial activity against test microorganisms	682:736	KGM/AgNP composite sponges displayed good antibacterial activity against test microorganisms.
29352894	1	1	theme	Konjac	156:161	arg1	sponge					216:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge was successfully prepared via a simple 2-step method for biomedical applications as wound-healing materials.
29352894	1	2	theme	2-step	262:267	arg1	method					269:274	a simple 2-step method	253:274	a simple 2-step method for biomedical applications as wound-healing materials	253:329	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge was successfully prepared via a simple 2-step method for biomedical applications as wound-healing materials.
29352894	0	3	theme	composite	73:81	arg1	sponge					83:88	a Konjac glucomannan/silver nanoparticle composite sponge	32:88	a Konjac glucomannan/silver nanoparticle composite sponge	32:88	A novel wound dressing based on a Konjac glucomannan/silver nanoparticle composite sponge effectively kills bacteria and accelerates wound healing.
29352894	5	4	theme	antibacterial	687:699	arg1	activity					701:708	good antibacterial activity	682:708	good antibacterial activity against test microorganisms	682:736	KGM/AgNP composite sponges displayed good antibacterial activity against test microorganisms.
29352894	1	5	theme	glucomannan/silver	163:180	arg1	sponge					216:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge was successfully prepared via a simple 2-step method for biomedical applications as wound-healing materials.
29352894	7	6	theme	KGM/AgNP	931:938	arg1	sponges					950:956	the KGM/AgNP composite sponges	927:956	the KGM/AgNP composite sponges	927:956	Animal models showed that the KGM/AgNP composite sponges effectively accelerated wound healing, and histological findings showed that they promoted fibroblast growth and accelerated epithelialization.
29352894	4	7	theme	water	588:592	arg1	retention					594:602	water retention	588:602	water retention	588:602	The KGM/AgNP composite sponge showed excellent water absorption and water retention, and considerable mechanical properties.
29352894	8	8	theme	composite	1148:1156	arg1	sponges					1158:1164	KGM/AgNP composite sponges	1139:1164	KGM/AgNP composite sponges	1139:1164	The experimental results showed that KGM/AgNP composite sponges have great potential in promoting wound healing.
29352894	7	9	theme	fibroblast	1049:1058	arg1	growth					1060:1065	fibroblast growth	1049:1065	fibroblast growth	1049:1065	Animal models showed that the KGM/AgNP composite sponges effectively accelerated wound healing, and histological findings showed that they promoted fibroblast growth and accelerated epithelialization.
29352894	7	10	theme	composite	940:948	arg1	sponges					950:956	the KGM/AgNP composite sponges	927:956	the KGM/AgNP composite sponges	927:956	Animal models showed that the KGM/AgNP composite sponges effectively accelerated wound healing, and histological findings showed that they promoted fibroblast growth and accelerated epithelialization.
29352894	8	11	theme	KGM/AgNP	1139:1146	arg1	sponges					1158:1164	KGM/AgNP composite sponges	1139:1164	KGM/AgNP composite sponges	1139:1164	The experimental results showed that KGM/AgNP composite sponges have great potential in promoting wound healing.
29352894	6	12	dep	In	739:740	arg1	vitro					742:746	vitro	742:746	vitro	742:746	In vitro cytocompatibility testing showed that L929 cells could survive well in the presence of KGM/AgNPs, indicating that KGM/AgNPs have good cytocompatibility.
29352894	1	13	theme	biomedical	280:289	arg1	applications					291:302	biomedical applications	280:302	biomedical applications as wound-healing materials	280:329	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge was successfully prepared via a simple 2-step method for biomedical applications as wound-healing materials.
29352894	5	14	theme	KGM/AgNP	645:652	arg1	sponges					664:670	KGM/AgNP composite sponges	645:670	KGM/AgNP composite sponges	645:670	KGM/AgNP composite sponges displayed good antibacterial activity against test microorganisms.
29352894	1	15	theme	KGM/AgNP	196:203	arg1	sponge					216:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge was successfully prepared via a simple 2-step method for biomedical applications as wound-healing materials.
29352894	1	16	theme	nanoparticle	182:193	arg1	sponge					216:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge was successfully prepared via a simple 2-step method for biomedical applications as wound-healing materials.
29352894	5	17	theme	composite	654:662	arg1	sponges					664:670	KGM/AgNP composite sponges	645:670	KGM/AgNP composite sponges	645:670	KGM/AgNP composite sponges displayed good antibacterial activity against test microorganisms.
29352894	6	18	theme	cytocompatibility	748:764	arg1	testing					766:772	In vitro cytocompatibility testing	739:772	In vitro cytocompatibility testing	739:772	In vitro cytocompatibility testing showed that L929 cells could survive well in the presence of KGM/AgNPs, indicating that KGM/AgNPs have good cytocompatibility.
29352894	0	19	theme	novel	2:6	arg1	dressing					14:21	A novel wound dressing	0:21	A novel wound dressing based on a Konjac glucomannan/silver nanoparticle composite sponge	0:88	A novel wound dressing based on a Konjac glucomannan/silver nanoparticle composite sponge effectively kills bacteria and accelerates wound healing.
29352894	6	20	contain	have	872:875	arg1	KGM/AgNPs					862:870	KGM/AgNPs	862:870	KGM/AgNPs	862:870	In vitro cytocompatibility testing showed that L929 cells could survive well in the presence of KGM/AgNPs, indicating that KGM/AgNPs have good cytocompatibility.
29352894	6	20	contain	have	872:875	arg2	cytocompatibility					882:898	good cytocompatibility	877:898	good cytocompatibility	877:898	In vitro cytocompatibility testing showed that L929 cells could survive well in the presence of KGM/AgNPs, indicating that KGM/AgNPs have good cytocompatibility.
29352894	6	21	theme	KGM/AgNPs	835:843	arg1	presence					823:830	the presence	819:830	the presence of KGM/AgNPs	819:843	In vitro cytocompatibility testing showed that L929 cells could survive well in the presence of KGM/AgNPs, indicating that KGM/AgNPs have good cytocompatibility.
29352894	3	22	theme	AgNP	426:429	arg1	solution					431:438	the AgNP solution	422:438	the AgNP solution	422:438	Then, KGM powder was added to the AgNP solution and stirred vigorously, and the composite sponge was obtained by freeze-drying.
29352894	4	23	theme	water	567:571	arg1	absorption					573:582	excellent water absorption	557:582	excellent water absorption	557:582	The KGM/AgNP composite sponge showed excellent water absorption and water retention, and considerable mechanical properties.
29352894	8	24	theme	great	1171:1175	arg1	potential					1177:1185	great potential	1171:1185	great potential	1171:1185	The experimental results showed that KGM/AgNP composite sponges have great potential in promoting wound healing.
29352894	6	25	theme	In	739:740	arg1	testing					766:772	In vitro cytocompatibility testing	739:772	In vitro cytocompatibility testing	739:772	In vitro cytocompatibility testing showed that L929 cells could survive well in the presence of KGM/AgNPs, indicating that KGM/AgNPs have good cytocompatibility.
29352894	8	26	contain	have	1166:1169	arg2	potential					1177:1185	great potential	1171:1185	great potential	1171:1185	The experimental results showed that KGM/AgNP composite sponges have great potential in promoting wound healing.
29352894	8	26	contain	have	1166:1169	arg1	sponges					1158:1164	KGM/AgNP composite sponges	1139:1164	KGM/AgNP composite sponges	1139:1164	The experimental results showed that KGM/AgNP composite sponges have great potential in promoting wound healing.
29352894	4	27	theme	excellent	557:565	arg1	absorption					573:582	excellent water absorption	557:582	excellent water absorption	557:582	The KGM/AgNP composite sponge showed excellent water absorption and water retention, and considerable mechanical properties.
29352894	0	28	theme	wound	133:137	arg1	healing					139:145	wound healing	133:145	wound healing	133:145	A novel wound dressing based on a Konjac glucomannan/silver nanoparticle composite sponge effectively kills bacteria and accelerates wound healing.
29352894	7	29	theme	histological	1001:1012	arg1	findings					1014:1021	histological findings	1001:1021	histological findings	1001:1021	Animal models showed that the KGM/AgNP composite sponges effectively accelerated wound healing, and histological findings showed that they promoted fibroblast growth and accelerated epithelialization.
29352894	7	30	theme	Animal	901:906	arg1	models					908:913	Animal models	901:913	Animal models	901:913	Animal models showed that the KGM/AgNP composite sponges effectively accelerated wound healing, and histological findings showed that they promoted fibroblast growth and accelerated epithelialization.
29352894	1	31	theme	composite	206:214	arg1	sponge					216:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge was successfully prepared via a simple 2-step method for biomedical applications as wound-healing materials.
29352894	0	32	theme	wound	8:12	arg1	dressing					14:21	A novel wound dressing	0:21	A novel wound dressing based on a Konjac glucomannan/silver nanoparticle composite sponge	0:88	A novel wound dressing based on a Konjac glucomannan/silver nanoparticle composite sponge effectively kills bacteria and accelerates wound healing.
29352894	1	33	theme	wound-healing	307:319	arg1	materials					321:329	wound-healing materials	307:329	wound-healing materials	307:329	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge was successfully prepared via a simple 2-step method for biomedical applications as wound-healing materials.
29352894	2	34	theme	deoxidizer	370:379	arg1	egg					381:383	green deoxidizer egg white	364:389	green deoxidizer egg white	364:389	First, AgNPs were prepared with green deoxidizer egg white.
29352894	7	35	theme	wound	982:986	arg1	healing					988:994	wound healing	982:994	wound healing	982:994	Animal models showed that the KGM/AgNP composite sponges effectively accelerated wound healing, and histological findings showed that they promoted fibroblast growth and accelerated epithelialization.
29352894	4	36	theme	composite	533:541	arg1	sponge					543:548	The KGM/AgNP composite sponge	520:548	The KGM/AgNP composite sponge	520:548	The KGM/AgNP composite sponge showed excellent water absorption and water retention, and considerable mechanical properties.
29352894	8	37	theme	wound	1200:1204	arg1	healing					1206:1212	wound healing	1200:1212	wound healing	1200:1212	The experimental results showed that KGM/AgNP composite sponges have great potential in promoting wound healing.
29352894	0	38	theme	Konjac	34:39	arg1	sponge					83:88	a Konjac glucomannan/silver nanoparticle composite sponge	32:88	a Konjac glucomannan/silver nanoparticle composite sponge	32:88	A novel wound dressing based on a Konjac glucomannan/silver nanoparticle composite sponge effectively kills bacteria and accelerates wound healing.
29352894	4	39	theme	KGM/AgNP	524:531	arg1	sponge					543:548	The KGM/AgNP composite sponge	520:548	The KGM/AgNP composite sponge	520:548	The KGM/AgNP composite sponge showed excellent water absorption and water retention, and considerable mechanical properties.
29352894	5	40	theme	test	718:721	arg1	microorganisms					723:736	test microorganisms	718:736	test microorganisms	718:736	KGM/AgNP composite sponges displayed good antibacterial activity against test microorganisms.
29352894	2	41	theme	green	364:368	arg1	egg					381:383	green deoxidizer egg white	364:389	green deoxidizer egg white	364:389	First, AgNPs were prepared with green deoxidizer egg white.
29352894	8	42	theme	experimental	1106:1117	arg1	results					1119:1125	The experimental results	1102:1125	The experimental results	1102:1125	The experimental results showed that KGM/AgNP composite sponges have great potential in promoting wound healing.
29352894	3	43	theme	composite	472:480	arg1	sponge					482:487	the composite sponge	468:487	the composite sponge	468:487	Then, KGM powder was added to the AgNP solution and stirred vigorously, and the composite sponge was obtained by freeze-drying.
29352894	4	44	theme	mechanical	622:631	arg1	properties					633:642	considerable mechanical properties	609:642	considerable mechanical properties	609:642	The KGM/AgNP composite sponge showed excellent water absorption and water retention, and considerable mechanical properties.
29352894	1	45	theme	novel	150:154	arg1	sponge					216:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge	148:221	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge was successfully prepared via a simple 2-step method for biomedical applications as wound-healing materials.
29352894	4	46	theme	considerable	609:620	arg1	properties					633:642	considerable mechanical properties	609:642	considerable mechanical properties	609:642	The KGM/AgNP composite sponge showed excellent water absorption and water retention, and considerable mechanical properties.
29352894	0	47	theme	nanoparticle	60:71	arg1	sponge					83:88	a Konjac glucomannan/silver nanoparticle composite sponge	32:88	a Konjac glucomannan/silver nanoparticle composite sponge	32:88	A novel wound dressing based on a Konjac glucomannan/silver nanoparticle composite sponge effectively kills bacteria and accelerates wound healing.
29352894	6	48	theme	good	877:880	arg1	cytocompatibility					882:898	good cytocompatibility	877:898	good cytocompatibility	877:898	In vitro cytocompatibility testing showed that L929 cells could survive well in the presence of KGM/AgNPs, indicating that KGM/AgNPs have good cytocompatibility.
29352894	3	49	theme	KGM	398:400	arg1	powder					402:407	KGM powder	398:407	KGM powder	398:407	Then, KGM powder was added to the AgNP solution and stirred vigorously, and the composite sponge was obtained by freeze-drying.
29352894	0	50	theme	glucomannan/silver	41:58	arg1	sponge					83:88	a Konjac glucomannan/silver nanoparticle composite sponge	32:88	a Konjac glucomannan/silver nanoparticle composite sponge	32:88	A novel wound dressing based on a Konjac glucomannan/silver nanoparticle composite sponge effectively kills bacteria and accelerates wound healing.
29352894	6	51	theme	L929	786:789	arg1	cells					791:795	L929 cells	786:795	L929 cells	786:795	In vitro cytocompatibility testing showed that L929 cells could survive well in the presence of KGM/AgNPs, indicating that KGM/AgNPs have good cytocompatibility.
29352894	2	52	theme	white	385:389	arg1	egg					381:383	green deoxidizer egg white	364:389	green deoxidizer egg white	364:389	First, AgNPs were prepared with green deoxidizer egg white.
29352894	1	53	theme	simple	255:260	arg1	method					269:274	a simple 2-step method	253:274	a simple 2-step method for biomedical applications as wound-healing materials	253:329	A novel Konjac glucomannan/silver nanoparticle (KGM/AgNP) composite sponge was successfully prepared via a simple 2-step method for biomedical applications as wound-healing materials.
26187189	4	0	theme	blend	734:738	arg1	films					740:744	the A/A/C blend films	724:744	the A/A/C blend films	724:744	In addition, the A/A/C blend films formed efficient hydrogel film with the water holding capacity of 23.6 times of their weight.
26187189	7	1	theme	fresh	1469:1473	arg1	agriculture					1475:1485	highly respiring fresh agriculture	1452:1485	highly respiring fresh agriculture produce	1452:1493	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	0	2	theme	films	94:98	arg1	application					16:26	application	16:26	application	16:26	Preparation and application of agar/alginate/collagen ternary blend functional food packaging films.
26187189	0	2	theme	films	94:98	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and application of agar/alginate/collagen ternary blend functional food packaging films.
26187189	4	3	theme	A/A/C	728:732	arg1	films					740:744	the A/A/C blend films	724:744	the A/A/C blend films	724:744	In addition, the A/A/C blend films formed efficient hydrogel film with the water holding capacity of 23.6 times of their weight.
26187189	6	4	theme	potatoes	1281:1288	arg1	greening					1269:1276	greening	1269:1276	greening of potatoes	1269:1288	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	6	5	theme	blend	1131:1135	arg1	films					1137:1141	all the A/A/C ternary blend films	1109:1141	all the A/A/C ternary blend films	1109:1141	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	3	6	theme	A/A/C	467:471	arg1	film					473:476	The A/A/C film	463:476	The A/A/C film	463:476	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	3	6	theme	A/A/C	467:471	arg1	transparent					489:499	transparent	489:499	transparent	489:499	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	7	7	theme	packaging	1523:1531	arg1	system					1533:1538	an active food packaging system	1508:1538	an active food packaging system utilizing their strong antimicrobial activity	1508:1584	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	4	8	theme	times	817:821	arg1	capacity					800:807	the water holding capacity	782:807	the water holding capacity of 23.6 times of their weight	782:837	In addition, the A/A/C blend films formed efficient hydrogel film with the water holding capacity of 23.6 times of their weight.
26187189	6	9	theme	ternary	1123:1129	arg1	films					1137:1141	all the A/A/C ternary blend films	1109:1141	all the A/A/C ternary blend films	1109:1141	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	2	10	theme	tensile	288:294	arg1	TS					306:307	TS	306:307	TS	306:307	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	2	10	theme	tensile	288:294	arg1	strength					296:303	tensile strength	288:303	tensile strength (TS)	288:308	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	6	11	theme	film	1196:1199	arg1	surface					1201:1207	the packaged film surface	1183:1207	the packaged film surface	1183:1207	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	6	12	theme	A/A/C	1117:1121	arg1	films					1137:1141	all the A/A/C ternary blend films	1109:1141	all the A/A/C ternary blend films	1109:1141	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	3	13	contain	had	643:645	arg1	film					638:641	the A/A/C(GSE) film	623:641	the A/A/C(GSE) film	623:641	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	3	13	contain	had	643:645	arg2	effect					665:670	high UV-screening effect	647:670	high UV-screening effect	647:670	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	7	14	theme	active	1511:1516	arg1	system					1533:1538	an active food packaging system	1508:1538	an active food packaging system utilizing their strong antimicrobial activity	1508:1584	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	2	15	theme	antimicrobial	423:435	arg1	activity					437:444	antimicrobial activity	423:444	antimicrobial activity	423:444	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	3	16	theme	UV-screening	590:601	arg1	effect					603:608	UV-screening effect	590:608	UV-screening effect	590:608	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	1	17	with	films	155:159	arg1	extract					215:221	grapefruit seed extract	199:221	grapefruit seed extract (GSE)	199:227	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	1	17	with	films	155:159	arg1	AgNPs					188:192	AgNPs	188:192	AgNPs	188:192	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	1	17	with	films	155:159	arg1	nanoparticles					173:185	silver nanoparticles	166:185	silver nanoparticles (AgNPs)	166:193	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	1	17	with	films	155:159	arg1	GSE					224:226	GSE	224:226	GSE	224:226	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	5	18	dep	Gram-negative	985:997	arg1	coli					1012:1015	Escherichia coli	1000:1015	Escherichia coli	1000:1015	Both A/A/C(AgNPs) and A/A/C(GSE) composite films exhibited strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria.
26187189	5	19	theme	Listeria	957:964	arg1	Gram-positive					942:954	Gram-positive	942:954	Gram-positive	942:954	Both A/A/C(AgNPs) and A/A/C(GSE) composite films exhibited strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria.
26187189	5	19	theme	Listeria	957:964	arg1	monocytogenes					966:978	Listeria monocytogenes	957:978	Listeria monocytogenes	957:978	Both A/A/C(AgNPs) and A/A/C(GSE) composite films exhibited strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria.
26187189	4	20	theme	weight	832:837	arg1	times					817:821	23.6 times	812:821	23.6 times of their weight	812:837	In addition, the A/A/C blend films formed efficient hydrogel film with the water holding capacity of 23.6 times of their weight.
26187189	1	21	theme	Ternary	101:107	arg1	films					155:159	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films	101:159	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE)	101:227	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	6	22	theme	condensed	1164:1172	arg1	water					1174:1178	condensed water	1164:1178	condensed water	1164:1178	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	6	23	theme	test	1054:1057	arg1	results					1059:1065	The test results	1050:1065	The test results of fresh potatoes packaging	1050:1093	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	3	24	theme	A/A/C	627:631	arg1	film					638:641	the A/A/C(GSE) film	623:641	the A/A/C(GSE) film	623:641	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	7	25	theme	food	1518:1521	arg1	system					1533:1538	an active food packaging system	1508:1538	an active food packaging system utilizing their strong antimicrobial activity	1508:1584	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	1	26	theme	blend	109:113	arg1	films					155:159	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films	101:159	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE)	101:227	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	6	27	theme	packaged	1187:1194	arg1	surface					1201:1207	the packaged film surface	1183:1207	the packaged film surface	1183:1207	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	1	28	theme	grapefruit	199:208	arg1	extract					215:221	grapefruit seed extract	199:221	grapefruit seed extract (GSE)	199:227	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	1	28	theme	grapefruit	199:208	arg1	GSE					224:226	GSE	224:226	GSE	224:226	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	3	29	theme	GSE	521:523	arg1	films					544:548	GSE incorporated blend films	521:548	GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE))	521:578	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	4	30	theme	water	786:790	arg1	capacity					800:807	the water holding capacity	782:807	the water holding capacity of 23.6 times of their weight	782:837	In addition, the A/A/C blend films formed efficient hydrogel film with the water holding capacity of 23.6 times of their weight.
26187189	1	31	theme	agar/alginate/collagen	115:136	arg1	films					155:159	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films	101:159	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE)	101:227	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	2	32	theme	swelling	375:382	arg1	SR					391:392	SR	391:392	SR	391:392	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	2	32	theme	swelling	375:382	arg1	ratio					384:388	water swelling ratio	369:388	water swelling ratio (SR)	369:393	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	6	33	theme	composite	1243:1251	arg1	films					1253:1257	A/A/C(GSE) composite films	1232:1257	A/A/C(GSE) composite films	1232:1257	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	6	34	theme	A/A/C	1232:1236	arg1	films					1253:1257	A/A/C(GSE) composite films	1232:1257	A/A/C(GSE) composite films	1232:1257	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	5	35	theme	strong	899:904	arg1	activity					920:927	strong antimicrobial activity	899:927	strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria	899:1047	Both A/A/C(AgNPs) and A/A/C(GSE) composite films exhibited strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria.
26187189	2	36	theme	water	396:400	arg1	WS					414:415	WS	414:415	WS	414:415	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	2	36	theme	water	396:400	arg1	solubility					402:411	water solubility	396:411	water solubility (WS)	396:416	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	2	37	theme	water	369:373	arg1	SR					391:392	SR	391:392	SR	391:392	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	2	37	theme	water	369:373	arg1	ratio					384:388	water swelling ratio	369:388	water swelling ratio (SR)	369:393	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	0	38	theme	ternary	54:60	arg1	films					94:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	Preparation and application of agar/alginate/collagen ternary blend functional food packaging films.
26187189	6	39	dep	surface	1201:1207	arg1	both					1210:1213	both	1210:1213	both	1210:1213	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	5	40	theme	antimicrobial	906:918	arg1	activity					920:927	strong antimicrobial activity	899:927	strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria	899:1047	Both A/A/C(AgNPs) and A/A/C(GSE) composite films exhibited strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria.
26187189	6	41	theme	GSE	1238:1240	arg1	films					1253:1257	A/A/C(GSE) composite films	1232:1257	A/A/C(GSE) composite films	1232:1257	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	2	42	theme	contact	349:355	arg1	CA					364:365	CA	364:365	CA	364:365	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	2	42	theme	contact	349:355	arg1	angle					357:361	water contact angle	343:361	water contact angle (CA)	343:366	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	5	43	dep	A/A/C	845:849	arg1	films					883:887	composite films	873:887	composite films	873:887	Both A/A/C(AgNPs) and A/A/C(GSE) composite films exhibited strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria.
26187189	0	44	theme	agar/alginate/collagen	31:52	arg1	films					94:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	Preparation and application of agar/alginate/collagen ternary blend functional food packaging films.
26187189	7	45	theme	packaging	1432:1440	arg1	films					1442:1446	packaging films	1432:1446	packaging films for highly respiring fresh agriculture produce	1432:1493	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	4	46	theme	holding	792:798	arg1	capacity					800:807	the water holding capacity	782:807	the water holding capacity of 23.6 times of their weight	782:837	In addition, the A/A/C blend films formed efficient hydrogel film with the water holding capacity of 23.6 times of their weight.
26187189	7	47	theme	hydrogel	1350:1357	arg1	films					1359:1363	the ternary blend hydrogel films	1332:1363	the ternary blend hydrogel films incorporated with AgNPs or GSE	1332:1394	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	6	48	theme	packaging	1085:1093	arg1	results					1059:1065	The test results	1050:1065	The test results of fresh potatoes packaging	1050:1093	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	3	49	dep	films	544:548	arg1	GSE					574:576	GSE	574:576	GSE	574:576	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	3	49	dep	films	544:548	arg1	AgNPs					557:561	AgNPs	557:561	AgNPs	557:561	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	3	49	dep	films	544:548	arg1	A/A/C					568:572	A/A/C	568:572	A/A/C(GSE)	568:577	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	3	49	dep	films	544:548	arg1	A/A/C					551:555	A/A/C	551:555	A/A/C(AgNPs)	551:562	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	0	50	theme	functional	68:77	arg1	films					94:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	Preparation and application of agar/alginate/collagen ternary blend functional food packaging films.
26187189	4	51	theme	hydrogel	763:770	arg1	film					772:775	efficient hydrogel film	753:775	efficient hydrogel film	753:775	In addition, the A/A/C blend films formed efficient hydrogel film with the water holding capacity of 23.6 times of their weight.
26187189	5	52	theme	pathogenic	1029:1038	arg1	bacteria					1040:1047	food-borne pathogenic bacteria	1018:1047	food-borne pathogenic bacteria	1018:1047	Both A/A/C(AgNPs) and A/A/C(GSE) composite films exhibited strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria.
26187189	6	53	theme	potatoes	1076:1083	arg1	packaging					1085:1093	fresh potatoes packaging	1070:1093	fresh potatoes packaging	1070:1093	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	1	54	theme	A/A/C	139:143	arg1	films					155:159	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films	101:159	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE)	101:227	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	0	55	theme	blend	62:66	arg1	films					94:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	Preparation and application of agar/alginate/collagen ternary blend functional food packaging films.
26187189	3	56	theme	GSE	633:635	arg1	film					638:641	the A/A/C(GSE) film	623:641	the A/A/C(GSE) film	623:641	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	4	57	theme	efficient	753:761	arg1	film					772:775	efficient hydrogel film	753:775	efficient hydrogel film	753:775	In addition, the A/A/C blend films formed efficient hydrogel film with the water holding capacity of 23.6 times of their weight.
26187189	7	58	theme	ternary	1336:1342	arg1	films					1359:1363	the ternary blend hydrogel films	1332:1363	the ternary blend hydrogel films incorporated with AgNPs or GSE	1332:1394	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	6	59	theme	fresh	1070:1074	arg1	packaging					1085:1093	fresh potatoes packaging	1070:1093	fresh potatoes packaging	1070:1093	The test results of fresh potatoes packaging revealed that all the A/A/C ternary blend films prevented forming of condensed water on the packaged film surface, both A/A/C(AgNPs) and A/A/C(GSE) composite films prevented greening of potatoes during storage.
26187189	7	60	theme	antimicrobial	1563:1575	arg1	activity					1577:1584	their strong antimicrobial activity	1550:1584	their strong antimicrobial activity	1550:1584	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	5	61	dep	Gram-positive	942:954	arg1	bacteria					1040:1047	food-borne pathogenic bacteria	1018:1047	food-borne pathogenic bacteria	1018:1047	Both A/A/C(AgNPs) and A/A/C(GSE) composite films exhibited strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria.
26187189	5	62	theme	composite	873:881	arg1	films					883:887	composite films	873:887	composite films	873:887	Both A/A/C(AgNPs) and A/A/C(GSE) composite films exhibited strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria.
26187189	3	63	theme	blend	538:542	arg1	films					544:548	GSE incorporated blend films	521:548	GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE))	521:578	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	1	64	theme	hydrogel	146:153	arg1	films					155:159	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films	101:159	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE)	101:227	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	7	65	theme	respiring	1459:1467	arg1	agriculture					1475:1485	highly respiring fresh agriculture	1452:1485	highly respiring fresh agriculture produce	1452:1493	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	7	66	dep	agriculture	1475:1485	arg1	produce					1487:1493	produce	1487:1493	produce	1487:1493	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	2	67	theme	water	311:315	arg1	permeability					323:334	water vapor permeability	311:334	water vapor permeability (WVP)	311:340	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	2	67	theme	water	311:315	arg1	WVP					337:339	WVP	337:339	WVP	337:339	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	2	68	theme	vapor	317:321	arg1	permeability					323:334	water vapor permeability	311:334	water vapor permeability (WVP)	311:340	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	2	68	theme	vapor	317:321	arg1	WVP					337:339	WVP	337:339	WVP	337:339	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	0	69	theme	packaging	84:92	arg1	films					94:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	Preparation and application of agar/alginate/collagen ternary blend functional food packaging films.
26187189	2	70	theme	performance	250:260	arg1	properties					262:271	Their performance properties	244:271	Their performance properties	244:271	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	1	71	theme	seed	210:213	arg1	extract					215:221	grapefruit seed extract	199:221	grapefruit seed extract (GSE)	199:227	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	1	71	theme	seed	210:213	arg1	GSE					224:226	GSE	224:226	GSE	224:226	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	2	72	theme	water	343:347	arg1	CA					364:365	CA	364:365	CA	364:365	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	2	72	theme	water	343:347	arg1	angle					357:361	water contact angle	343:361	water contact angle (CA)	343:366	Their performance properties, transparency, tensile strength (TS), water vapor permeability (WVP), water contact angle (CA), water swelling ratio (SR), water solubility (WS), and antimicrobial activity were determined.
26187189	5	73	theme	food-borne	1018:1027	arg1	bacteria					1040:1047	food-borne pathogenic bacteria	1018:1047	food-borne pathogenic bacteria	1018:1047	Both A/A/C(AgNPs) and A/A/C(GSE) composite films exhibited strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli) food-borne pathogenic bacteria.
26187189	3	74	theme	high	647:650	arg1	effect					665:670	high UV-screening effect	647:670	high UV-screening effect	647:670	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	0	75	theme	food	79:82	arg1	films					94:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	agar/alginate/collagen ternary blend functional food packaging films	31:98	Preparation and application of agar/alginate/collagen ternary blend functional food packaging films.
26187189	7	76	used	used	1403:1406	arg2	films					1359:1363	the ternary blend hydrogel films	1332:1363	the ternary blend hydrogel films incorporated with AgNPs or GSE	1332:1394	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	3	77	theme	incorporated	525:536	arg1	films					544:548	GSE incorporated blend films	521:548	GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE))	521:578	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
26187189	7	78	theme	blend	1344:1348	arg1	films					1359:1363	the ternary blend hydrogel films	1332:1363	the ternary blend hydrogel films incorporated with AgNPs or GSE	1332:1394	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	1	79	theme	silver	166:171	arg1	AgNPs					188:192	AgNPs	188:192	AgNPs	188:192	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	1	79	theme	silver	166:171	arg1	nanoparticles					173:185	silver nanoparticles	166:185	silver nanoparticles (AgNPs)	166:193	Ternary blend agar/alginate/collagen (A/A/C) hydrogel films with silver nanoparticles (AgNPs) and grapefruit seed extract (GSE) were prepared.
26187189	7	80	theme	strong	1556:1561	arg1	activity					1577:1584	their strong antimicrobial activity	1550:1584	their strong antimicrobial activity	1550:1584	The results indicate that the ternary blend hydrogel films incorporated with AgNPs or GSE can be used not only as antifogging packaging films for highly respiring fresh agriculture produce, but also as an active food packaging system utilizing their strong antimicrobial activity.
26187189	3	81	theme	UV-screening	652:663	arg1	effect					665:670	high UV-screening effect	647:670	high UV-screening effect	647:670	The A/A/C film was highly transparent, and both AgNPs and GSE incorporated blend films (A/A/C(AgNPs) and A/A/C(GSE)) exhibited UV-screening effect, especially, the A/A/C(GSE) film had high UV-screening effect without sacrificing the transmittance.
24928668	4	0	theme	implanted	847:855	arg1	group					857:861	a powder gel implanted group	834:861	a powder gel implanted group	834:861	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	7	1	theme	powder	1082:1087	arg1	composite					1093:1101	The rhBMP-2 powder gel composite	1070:1101	The rhBMP-2 powder gel composite	1070:1101	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	2	2	theme	powder-like	395:405	arg1	composite					416:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite	336:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel)	336:437	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	2	theme	powder-like	395:405	arg1	gel					434:436	powder gel	427:436	powder gel	427:436	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	2	theme	powder-like	395:405	arg1	carrier					471:477	an effective rhBMP-2 carrier	450:477	an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality	450:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	9	3	theme	only	1604:1607	arg1	group					1609:1613	the implant only group	1592:1613	the implant only group	1592:1613	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	3	4	theme	release	554:560	arg1	pattern					562:568	The release pattern	550:568	The release pattern for rhBMP-2	550:580	The release pattern for rhBMP-2 was then evaluated against an rhBMP-2-loaded collagen sponge as a control group.
24928668	3	4	theme	release	554:560	arg1	group					656:660	a control group	646:660	a control group	646:660	The release pattern for rhBMP-2 was then evaluated against an rhBMP-2-loaded collagen sponge as a control group.
24928668	4	5	theme	powder	836:841	arg1	group					857:861	a powder gel implanted group	834:861	a powder gel implanted group	834:861	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	9	6	theme	powder	1623:1628	arg1	group					1644:1648	the powder gel implanted group	1619:1648	the powder gel implanted group	1619:1648	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	0	7	theme	osseointegration	127:142	arg1	evaluation					105:114	evaluation	105:114	evaluation of implant osseointegration	105:142	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	0	7	theme	osseointegration	127:142	arg1	osseointegration					127:142	implant osseointegration	119:142	implant osseointegration	119:142	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	0	7	theme	osseointegration	127:142	arg1	composite					91:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	10	8	theme	good	1683:1686	arg1	carrier					1688:1694	a good carrier	1681:1694	a good carrier	1681:1694	The powder gel appeared to be a good carrier and could release rhBMP-2 slowly to promote the formation of new bone following implantation in a bone defect, thereby improving implant osseointegration.
24928668	10	8	theme	good	1683:1686	arg1	gel					1662:1664	The powder gel	1651:1664	The powder gel	1651:1664	The powder gel appeared to be a good carrier and could release rhBMP-2 slowly to promote the formation of new bone following implantation in a bone defect, thereby improving implant osseointegration.
24928668	8	9	from	osseointegration	1366:1381	arg1	bone					1399:1402	the cortical bone	1386:1402	the cortical bone	1386:1402	The histology results indicated that the rhBMP-2 powder gel composite improved the osseointegration in the cortical bone as well as the marrow space along the fixture.
24928668	9	10	theme	implanted	1634:1642	arg1	group					1644:1648	the powder gel implanted group	1619:1648	the powder gel implanted group	1619:1648	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	8	11	theme	cortical	1390:1397	arg1	bone					1399:1402	the cortical bone	1386:1402	the cortical bone	1386:1402	The histology results indicated that the rhBMP-2 powder gel composite improved the osseointegration in the cortical bone as well as the marrow space along the fixture.
24928668	3	12	theme	collagen	627:634	arg1	sponge					636:641	an rhBMP-2-loaded collagen sponge	609:641	an rhBMP-2-loaded collagen sponge	609:641	The release pattern for rhBMP-2 was then evaluated against an rhBMP-2-loaded collagen sponge as a control group.
24928668	1	13	theme	carriers	240:247	arg1	kinds					231:235	various kinds	223:235	various kinds of carriers	223:247	Methods to improve osseointegration that include implantation of rhBMP-2 with various kinds of carriers are currently of considerable interest.
24928668	7	14	from	amount	1169:1174	arg1	pitch					1213:1217	the implant pitch	1201:1217	the implant pitch	1201:1217	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	5	15	theme	implanted	894:902	arg1	areas					904:908	the implanted areas	890:908	the implanted areas	890:908	Micro-CT and histology of the implanted areas were carried out four weeks later.
24928668	3	16	theme	control	648:654	arg1	pattern					562:568	The release pattern	550:568	The release pattern for rhBMP-2	550:580	The release pattern for rhBMP-2 was then evaluated against an rhBMP-2-loaded collagen sponge as a control group.
24928668	3	16	theme	control	648:654	arg1	group					656:660	a control group	646:660	a control group	646:660	The release pattern for rhBMP-2 was then evaluated against an rhBMP-2-loaded collagen sponge as a control group.
24928668	8	17	theme	marrow	1419:1424	arg1	space					1426:1430	the marrow space	1415:1430	the osseointegration in the cortical bone as well as the marrow space	1362:1430	The histology results indicated that the rhBMP-2 powder gel composite improved the osseointegration in the cortical bone as well as the marrow space along the fixture.
24928668	9	18	dep	loaded	1509:1514	arg1	rhBMP-2					1492:1498	rhBMP-2	1492:1498	rhBMP-2 (200 µg)	1492:1507	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	9	18	dep	loaded	1509:1514	arg1	200 µg					1501:1506	200 µg	1501:1506	200 µg	1501:1506	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	7	19	theme	formation	1188:1196	arg1	amount					1169:1174	the amount	1165:1174	the amount of new bone formation in the implant pitch	1165:1217	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	7	19	theme	formation	1188:1196	arg1	formation					1188:1196	new bone formation	1179:1196	new bone formation	1179:1196	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	9	20	theme	bone-to-implant	1455:1469	arg1	higher					1571:1576	higher	1571:1576	higher	1571:1576	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	9	20	theme	bone-to-implant	1455:1469	arg1	ratio					1479:1483	The bone-to-implant contact ratio	1451:1483	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1451:1551	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	2	21	theme	bone	516:519	arg1	defect					521:526	a bone defect	514:526	a bone defect	514:526	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	5	22	dep	carried	915:921	arg1	out					923:925	out	923:925	out	923:925	Micro-CT and histology of the implanted areas were carried out four weeks later.
24928668	0	23	theme	gel	87:89	arg1	osseointegration					127:142	implant osseointegration	119:142	implant osseointegration	119:142	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	0	23	theme	gel	87:89	arg1	composite					91:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	1	24	dep	Methods	145:151	arg1	improve					156:162	improve	156:162	to improve osseointegration that include implantation of rhBMP-2 with various kinds of carriers	153:247	Methods to improve osseointegration that include implantation of rhBMP-2 with various kinds of carriers are currently of considerable interest.
24928668	4	25	dep	groups	723:728	arg1	200 µg					754:759	200 µg	754:759	200 µg	754:759	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	4	25	dep	groups	723:728	arg1	rhBMP-2					745:751	an rhBMP-2	742:751	three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group	717:861	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	6	26	theme	powder	957:962	arg1	gel					964:966	The rhBMP-2 powder gel	945:966	The rhBMP-2 powder gel	945:966	The rhBMP-2 powder gel released less rhBMP-2 than the collagen sponge, but it continued a slow release for more than 7 days.
24928668	2	27	theme	bone	536:539	arg1	quality					541:547	poor bone quality	531:547	poor bone quality	531:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	4	28	theme	gel	776:778	arg1	group					800:804	powder gel composite implanted group	769:804	powder gel composite implanted group	769:804	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	7	29	theme	bone	1277:1280	arg1	quality					1244:1250	bone quality	1239:1250	bone quality	1239:1250	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	7	29	theme	bone	1277:1280	arg1	quantity					1261:1268	bone quantity	1256:1268	bone quantity	1256:1268	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	0	30	theme	powder	80:85	arg1	osseointegration					127:142	implant osseointegration	119:142	implant osseointegration	119:142	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	0	30	theme	powder	80:85	arg1	composite					91:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	7	31	theme	implant	1143:1149	arg1	osseointegration					1112:1127	osseointegration	1112:1127	osseointegration of the dental implant	1112:1149	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	8	32	theme	rhBMP-2	1324:1330	arg1	composite					1343:1351	the rhBMP-2 powder gel composite	1320:1351	the rhBMP-2 powder gel composite	1320:1351	The histology results indicated that the rhBMP-2 powder gel composite improved the osseointegration in the cortical bone as well as the marrow space along the fixture.
24928668	4	33	theme	implant	810:816	arg1	group					823:827	an implant only group	807:827	an implant only group	807:827	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	2	34	theme	effective	453:461	arg1	composite					416:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite	336:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel)	336:437	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	34	theme	effective	453:461	arg1	carrier					471:477	an effective rhBMP-2 carrier	450:477	an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality	450:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	9	35	theme	powder	1516:1521	arg1	group					1547:1551	the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1488:1551	the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1488:1551	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	7	36	theme	bone	1256:1259	arg1	quantity					1261:1268	bone quantity	1256:1268	bone quantity	1256:1268	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	8	37	theme	gel	1339:1341	arg1	composite					1343:1351	the rhBMP-2 powder gel composite	1320:1351	the rhBMP-2 powder gel composite	1320:1351	The histology results indicated that the rhBMP-2 powder gel composite improved the osseointegration in the cortical bone as well as the marrow space along the fixture.
24928668	9	38	theme	composite	1527:1535	arg1	group					1547:1551	the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1488:1551	the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1488:1551	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	2	39	theme	host	499:502	arg1	bone					504:507	host bone	499:507	host bone with a bone defect or poor bone quality	499:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	0	40	theme	acid-based	69:78	arg1	osseointegration					127:142	implant osseointegration	119:142	implant osseointegration	119:142	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	0	40	theme	acid-based	69:78	arg1	composite					91:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	2	41	theme	acid-based	384:393	arg1	composite					416:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite	336:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel)	336:437	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	41	theme	acid-based	384:393	arg1	gel					434:436	powder gel	427:436	powder gel	427:436	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	41	theme	acid-based	384:393	arg1	carrier					471:477	an effective rhBMP-2 carrier	450:477	an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality	450:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	0	42	theme	β-TCP	40:44	arg1	osseointegration					127:142	implant osseointegration	119:142	implant osseointegration	119:142	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	0	42	theme	β-TCP	40:44	arg1	composite					91:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	9	43	theme	group	1547:1551	arg1	higher					1571:1576	higher	1571:1576	higher	1571:1576	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	9	43	theme	group	1547:1551	arg1	ratio					1479:1483	The bone-to-implant contact ratio	1451:1483	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1451:1551	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	10	44	theme	bone	1794:1797	arg1	defect					1799:1804	a bone defect	1792:1804	a bone defect	1792:1804	The powder gel appeared to be a good carrier and could release rhBMP-2 slowly to promote the formation of new bone following implantation in a bone defect, thereby improving implant osseointegration.
24928668	4	45	theme	implanted	790:798	arg1	group					800:804	powder gel composite implanted group	769:804	powder gel composite implanted group	769:804	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	2	46	theme	β-TCP	355:359	arg1	composite					416:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite	336:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel)	336:437	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	46	theme	β-TCP	355:359	arg1	gel					434:436	powder gel	427:436	powder gel	427:436	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	46	theme	β-TCP	355:359	arg1	carrier					471:477	an effective rhBMP-2 carrier	450:477	an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality	450:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	7	47	dep	quality	1244:1250	arg1	the					1235:1237	the	1235:1237	the	1235:1237	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	0	48	theme	microsphere-hyaluronic	46:67	arg1	osseointegration					127:142	implant osseointegration	119:142	implant osseointegration	119:142	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	0	48	theme	microsphere-hyaluronic	46:67	arg1	composite					91:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	7	49	theme	rhBMP-2	1074:1080	arg1	composite					1093:1101	The rhBMP-2 powder gel composite	1070:1101	The rhBMP-2 powder gel composite	1070:1101	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	2	50	theme	hydrogel	407:414	arg1	composite					416:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite	336:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel)	336:437	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	50	theme	hydrogel	407:414	arg1	gel					434:436	powder gel	427:436	powder gel	427:436	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	50	theme	hydrogel	407:414	arg1	carrier					471:477	an effective rhBMP-2 carrier	450:477	an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality	450:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	4	51	theme	gel	843:845	arg1	group					857:861	a powder gel implanted group	834:861	a powder gel implanted group	834:861	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	10	52	theme	new	1757:1759	arg1	bone					1761:1764	new bone	1757:1764	new bone following implantation	1757:1787	The powder gel appeared to be a good carrier and could release rhBMP-2 slowly to promote the formation of new bone following implantation in a bone defect, thereby improving implant osseointegration.
24928668	9	53	theme	implant	1596:1602	arg1	group					1609:1613	the implant only group	1592:1613	the implant only group	1592:1613	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	7	54	theme	new	1179:1181	arg1	formation					1188:1196	new bone formation	1179:1196	new bone formation	1179:1196	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	2	55	theme	rhBMP-2	340:346	arg1	composite					416:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite	336:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel)	336:437	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	55	theme	rhBMP-2	340:346	arg1	gel					434:436	powder gel	427:436	powder gel	427:436	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	55	theme	rhBMP-2	340:346	arg1	carrier					471:477	an effective rhBMP-2 carrier	450:477	an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality	450:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	1	56	theme	rhBMP-2	210:216	arg1	implantation					194:205	implantation	194:205	implantation of rhBMP-2 with various kinds of carriers	194:247	Methods to improve osseointegration that include implantation of rhBMP-2 with various kinds of carriers are currently of considerable interest.
24928668	6	57	dep	less	977:980	arg1	rhBMP-2					982:988	rhBMP-2	982:988	rhBMP-2	982:988	The rhBMP-2 powder gel released less rhBMP-2 than the collagen sponge, but it continued a slow release for more than 7 days.
24928668	6	58	theme	rhBMP-2	949:955	arg1	gel					964:966	The rhBMP-2 powder gel	945:966	The rhBMP-2 powder gel	945:966	The rhBMP-2 powder gel released less rhBMP-2 than the collagen sponge, but it continued a slow release for more than 7 days.
24928668	1	59	theme	various	223:229	arg1	kinds					231:235	various kinds	223:235	various kinds of carriers	223:247	Methods to improve osseointegration that include implantation of rhBMP-2 with various kinds of carriers are currently of considerable interest.
24928668	9	60	theme	gel	1630:1632	arg1	group					1644:1648	the powder gel implanted group	1619:1648	the powder gel implanted group	1619:1648	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	3	61	theme	rhBMP-2-loaded	612:625	arg1	sponge					636:641	an rhBMP-2-loaded collagen sponge	609:641	an rhBMP-2-loaded collagen sponge	609:641	The release pattern for rhBMP-2 was then evaluated against an rhBMP-2-loaded collagen sponge as a control group.
24928668	4	62	theme	Dental	663:668	arg1	implants					670:677	Dental implants	663:677	Dental implants	663:677	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	4	63	theme	groups	723:728	arg1	tibias					707:712	the tibias	703:712	the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group	703:861	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	2	64	theme	present	293:299	arg1	study					301:305	The present study	289:305	The present study	289:305	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	5	65	theme	areas	904:908	arg1	histology					877:885	histology	877:885	histology	877:885	Micro-CT and histology of the implanted areas were carried out four weeks later.
24928668	5	65	theme	areas	904:908	arg1	Micro-CT					864:871	Micro-CT	864:871	Micro-CT	864:871	Micro-CT and histology of the implanted areas were carried out four weeks later.
24928668	6	66	theme	collagen	999:1006	arg1	sponge					1008:1013	the collagen sponge	995:1013	the collagen sponge	995:1013	The rhBMP-2 powder gel released less rhBMP-2 than the collagen sponge, but it continued a slow release for more than 7 days.
24928668	4	67	theme	rabbits	733:739	arg1	rabbits					733:739	rabbits	733:739	rabbits	733:739	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	4	67	theme	rabbits	733:739	arg1	groups					723:728	three groups	717:728	three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group	717:861	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	7	68	theme	bone	1183:1186	arg1	formation					1188:1196	new bone formation	1179:1196	new bone formation	1179:1196	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	2	69	with	bone	504:507	arg1	defect					521:526	a bone defect	514:526	a bone defect	514:526	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	69	with	bone	504:507	arg1	quality					541:547	poor bone quality	531:547	poor bone quality	531:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	9	70	theme	contact	1471:1477	arg1	higher					1571:1576	higher	1571:1576	higher	1571:1576	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	9	70	theme	contact	1471:1477	arg1	ratio					1479:1483	The bone-to-implant contact ratio	1451:1483	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1451:1551	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	2	71	from	implantation	483:494	arg1	bone					504:507	host bone	499:507	host bone with a bone defect or poor bone quality	499:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	8	72	from	space	1426:1430	arg1	bone					1399:1402	the cortical bone	1386:1402	the cortical bone	1386:1402	The histology results indicated that the rhBMP-2 powder gel composite improved the osseointegration in the cortical bone as well as the marrow space along the fixture.
24928668	0	73	theme	implant	119:125	arg1	osseointegration					127:142	implant osseointegration	119:142	implant osseointegration	119:142	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	7	74	theme	new	1273:1275	arg1	bone					1277:1280	new bone	1273:1280	new bone	1273:1280	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	2	75	theme	poor	531:534	arg1	quality					541:547	poor bone quality	531:547	poor bone quality	531:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	8	76	theme	histology	1287:1295	arg1	results					1297:1303	The histology results	1283:1303	The histology results	1283:1303	The histology results indicated that the rhBMP-2 powder gel composite improved the osseointegration in the cortical bone as well as the marrow space along the fixture.
24928668	7	77	theme	dental	1136:1141	arg1	implant					1143:1149	the dental implant	1132:1149	the dental implant	1132:1149	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	2	78	theme	powder	427:432	arg1	composite					416:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite	336:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel)	336:437	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	78	theme	powder	427:432	arg1	gel					434:436	powder gel	427:436	powder gel	427:436	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	0	79	theme	rhBMP-2	18:24	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of an rhBMP-2	0:24	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	9	80	theme	loaded	1509:1514	arg1	group					1547:1551	the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1488:1551	the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1488:1551	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	2	81	theme	rhBMP-2	463:469	arg1	composite					416:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite	336:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel)	336:437	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	81	theme	rhBMP-2	463:469	arg1	carrier					471:477	an effective rhBMP-2 carrier	450:477	an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality	450:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	0	82	theme	porous	33:38	arg1	osseointegration					127:142	implant osseointegration	119:142	implant osseointegration	119:142	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	0	82	theme	porous	33:38	arg1	composite					91:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	porous β-TCP microsphere-hyaluronic acid-based powder gel composite	33:99	Fabrication of an rhBMP-2 loaded porous β-TCP microsphere-hyaluronic acid-based powder gel composite and evaluation of implant osseointegration.
24928668	9	83	theme	gel	1523:1525	arg1	group					1547:1551	the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1488:1551	the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1488:1551	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	7	84	theme	bone	1239:1242	arg1	quality					1244:1250	bone quality	1239:1250	bone quality	1239:1250	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	10	85	theme	bone	1761:1764	arg1	formation					1744:1752	the formation	1740:1752	the formation of new bone following implantation	1740:1787	The powder gel appeared to be a good carrier and could release rhBMP-2 slowly to promote the formation of new bone following implantation in a bone defect, thereby improving implant osseointegration.
24928668	8	86	theme	powder	1332:1337	arg1	composite					1343:1351	the rhBMP-2 powder gel composite	1320:1351	the rhBMP-2 powder gel composite	1320:1351	The histology results indicated that the rhBMP-2 powder gel composite improved the osseointegration in the cortical bone as well as the marrow space along the fixture.
24928668	7	87	theme	gel	1089:1091	arg1	composite					1093:1101	The rhBMP-2 powder gel composite	1070:1101	The rhBMP-2 powder gel composite	1070:1101	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	9	88	theme	implanted	1537:1545	arg1	group					1547:1551	the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1488:1551	the rhBMP-2 (200 µg) loaded powder gel composite implanted group	1488:1551	The bone-to-implant contact ratio of the rhBMP-2 (200 µg) loaded powder gel composite implanted group was significantly higher than those of the implant only group and the powder gel implanted group.
24928668	4	89	theme	only	818:821	arg1	group					823:827	an implant only group	807:827	an implant only group	807:827	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	2	90	theme	microsphere-hyaluronic	361:382	arg1	composite					416:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite	336:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel)	336:437	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	90	theme	microsphere-hyaluronic	361:382	arg1	gel					434:436	powder gel	427:436	powder gel	427:436	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	90	theme	microsphere-hyaluronic	361:382	arg1	carrier					471:477	an effective rhBMP-2 carrier	450:477	an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality	450:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	1	91	with	implantation	194:205	arg1	kinds					231:235	various kinds	223:235	various kinds of carriers	223:247	Methods to improve osseointegration that include implantation of rhBMP-2 with various kinds of carriers are currently of considerable interest.
24928668	4	92	theme	powder	769:774	arg1	group					800:804	powder gel composite implanted group	769:804	powder gel composite implanted group	769:804	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	4	93	theme	composite	780:788	arg1	group					800:804	powder gel composite implanted group	769:804	powder gel composite implanted group	769:804	Dental implants were also inserted into the tibias of three groups of rabbits: an rhBMP-2 (200 µg) loaded powder gel composite implanted group, an implant only group, and a powder gel implanted group.
24928668	2	94	theme	loaded	348:353	arg1	composite					416:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite	336:424	the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel)	336:437	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	94	theme	loaded	348:353	arg1	gel					434:436	powder gel	427:436	powder gel	427:436	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	2	94	theme	loaded	348:353	arg1	carrier					471:477	an effective rhBMP-2 carrier	450:477	an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality	450:547	The present study was conducted to evaluate if the rhBMP-2 loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective rhBMP-2 carrier for implantation in host bone with a bone defect or poor bone quality.
24928668	7	95	theme	implant	1205:1211	arg1	pitch					1213:1217	the implant pitch	1201:1217	the implant pitch	1201:1217	The rhBMP-2 powder gel composite improved osseointegration of the dental implant by increasing the amount of new bone formation in the implant pitch and it improved the bone quality and bone quantity of new bone.
24928668	6	96	theme	slow	1035:1038	arg1	release					1040:1046	a slow release	1033:1046	a slow release	1033:1046	The rhBMP-2 powder gel released less rhBMP-2 than the collagen sponge, but it continued a slow release for more than 7 days.
24928668	10	97	theme	implant	1825:1831	arg1	osseointegration					1833:1848	implant osseointegration	1825:1848	implant osseointegration	1825:1848	The powder gel appeared to be a good carrier and could release rhBMP-2 slowly to promote the formation of new bone following implantation in a bone defect, thereby improving implant osseointegration.
24928668	10	98	theme	powder	1655:1660	arg1	carrier					1688:1694	a good carrier	1681:1694	a good carrier	1681:1694	The powder gel appeared to be a good carrier and could release rhBMP-2 slowly to promote the formation of new bone following implantation in a bone defect, thereby improving implant osseointegration.
24928668	10	98	theme	powder	1655:1660	arg1	gel					1662:1664	The powder gel	1651:1664	The powder gel	1651:1664	The powder gel appeared to be a good carrier and could release rhBMP-2 slowly to promote the formation of new bone following implantation in a bone defect, thereby improving implant osseointegration.
24928668	1	99	theme	considerable	266:277	arg1	interest					279:286	considerable interest	266:286	considerable interest	266:286	Methods to improve osseointegration that include implantation of rhBMP-2 with various kinds of carriers are currently of considerable interest.
27795394	6	0	theme	antimicrobial	1109:1121	arg1	peptides					1123:1130	antimicrobial peptides	1109:1130	antimicrobial peptides	1109:1130	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	10	1	theme	A	2098:2098	arg1	change					2100:2105	lipid A change	2092:2105	lipid A change in Salmonella enterica serovar Typhimurium	2092:2148	We developed a system to track the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium following an environmental shift to PhoP/Q- and PmrA/B-inducing conditions.
27795394	8	2	theme	budded	1604:1609	arg1	OMVs					1611:1614	the OM and the newly budded OMVs	1583:1614	OMVs	1611:1614	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	7	3	theme	OMV	1472:1474	arg1	production					1476:1485	greater OMV production	1464:1485	greater OMV production	1464:1485	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	6	4	theme	lipid	1189:1193	arg1	A					1195:1195	the LPS lipid A	1181:1195	the LPS lipid A	1181:1195	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	6	5	theme	OM	1255:1256	arg1	proteins					1258:1265	specific OM proteins	1246:1265	specific OM proteins	1246:1265	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	3	6	theme	lipopolysaccharide	568:585	arg1	glycoforms					593:602	unfavorable lipopolysaccharide (LPS) glycoforms	556:602	unfavorable lipopolysaccharide (LPS) glycoforms	556:602	Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth.
27795394	11	7	theme	lipid	2293:2297	arg1	content					2301:2307	lipid A content	2293:2307	lipid A content	2293:2307	We show that growth conditions influence OMV production, size, and lipid A content.
27795394	8	8	from	present	1572:1578	arg1	OMVs					1611:1614	the OM and the newly budded OMVs	1583:1614	OMVs	1611:1614	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	8	8	from	present	1572:1578	arg1	OM					1587:1588	the OM and the newly budded OMVs	1583:1614	OM	1587:1588	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	2	9	theme	asymmetric	277:286	arg1	structure					306:314	the asymmetric and heterogeneous structure	273:314	the asymmetric and heterogeneous structure of the Gram-negative OM	273:338	However, the asymmetric and heterogeneous structure of the Gram-negative OM poses unique challenges to the cell's successful adaption to rapid environmental transitions.
27795394	8	10	theme	A	1697:1697	arg1	species					1699:1705	lipid A species	1691:1705	lipid A species	1691:1705	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	5	11	theme	OM	876:877	arg1	composition					879:889	OM composition	876:889	OM composition	876:889	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	12	12	theme	significant	2434:2444	arg1	retention					2446:2454	the significant retention	2430:2454	the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions	2430:2579	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	2	13	theme	Gram-negative	323:335	arg1	OM					337:338	the Gram-negative OM	319:338	the Gram-negative OM	319:338	However, the asymmetric and heterogeneous structure of the Gram-negative OM poses unique challenges to the cell's successful adaption to rapid environmental transitions.
27795394	12	14	theme	lipid	2459:2463	arg1	species					2467:2473	lipid A species	2459:2473	lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions	2459:2579	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	8	15	theme	phosphate	1721:1729	arg1	moieties					1731:1738	modified phosphate moieties	1712:1738	modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species	1712:1809	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	12	16	theme	lipid	2342:2346	arg1	content					2350:2356	the lipid A content	2338:2356	the lipid A content of OMVs	2338:2364	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	9	17	theme	LPS	1968:1970	arg1	composition					1953:1963	the structural composition	1938:1963	the structural composition of LPS present in their OM in response to various environmental stimuli	1938:2035	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	8	18	from	moieties	1731:1738	arg1	OM					1747:1748	the OM	1743:1748	the OM concomitant with the selective loss of palmitoylated species	1743:1809	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	7	19	theme	modified	1375:1382	arg1	species					1384:1390	modified species	1375:1390	modified species of LPS	1375:1397	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	10	20	theme	PhoP/Q-	2186:2192	arg1	conditions					2214:2223	PhoP/Q- and PmrA/B-inducing conditions	2186:2223	PhoP/Q- and PmrA/B-inducing conditions	2186:2223	We developed a system to track the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium following an environmental shift to PhoP/Q- and PmrA/B-inducing conditions.
27795394	3	21	gly	glycoforms	593:602	arg1	LPS					588:590	LPS	588:590	LPS	588:590	Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth.
27795394	3	21	gly	glycoforms	593:602	arg1	lipopolysaccharide					568:585	lipopolysaccharide	568:585	unfavorable lipopolysaccharide (LPS) glycoforms	556:602	Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth.
27795394	8	22	with	concomitant	1750:1760	arg1	loss					1781:1784	the selective loss	1767:1784	the selective loss of palmitoylated species	1767:1809	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	8	23	from	OMVs	1611:1614	arg1	present					1572:1578	present	1572:1578	present	1572:1578	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	14	24	theme	Gram-negative	2825:2837	arg1	bacteria					2839:2846	Gram-negative bacteria	2825:2846	Gram-negative bacteria	2825:2846	These results highlight a role for the OMV response in OM remodeling and maintenance processes in Gram-negative bacteria.
27795394	14	25	theme	maintenance	2800:2810	arg1	processes					2812:2820	maintenance processes	2800:2820	maintenance processes	2800:2820	These results highlight a role for the OMV response in OM remodeling and maintenance processes in Gram-negative bacteria.
27795394	11	26	theme	growth	2239:2244	arg1	conditions					2246:2255	growth conditions	2239:2255	growth conditions	2239:2255	We show that growth conditions influence OMV production, size, and lipid A content.
27795394	0	27	theme	Outer	65:69	arg1	Maintenance					80:90	Outer Membrane Maintenance	65:90	Outer Membrane Maintenance	65:90	Outer Membrane Vesicle Production Facilitates LPS Remodeling and Outer Membrane Maintenance in Salmonella during Environmental Transitions.
27795394	3	28	theme	cell	634:637	arg1	growth					639:644	cell growth	634:644	cell growth	634:644	Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth.
27795394	8	29	theme	selective	1771:1779	arg1	loss					1781:1784	the selective loss	1767:1784	the selective loss of palmitoylated species	1767:1809	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	5	30	theme	relevant	914:921	arg1	changes					937:943	physiologically relevant environmental changes	898:943	physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs)	898:1066	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	12	31	contain	containing	2475:2484	arg1	species					2467:2473	lipid A species	2459:2473	lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions	2459:2579	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	12	31	contain	containing	2475:2484	arg2	modifications					2495:2507	covalent modifications	2486:2507	covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions	2486:2579	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	9	32	theme	various	2007:2013	arg1	stimuli					2029:2035	various environmental stimuli	2007:2035	various environmental stimuli	2007:2035	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	8	33	theme	acidic	1861:1866	arg1	conditions					1882:1891	moderately acidic environmental conditions	1850:1891	moderately acidic environmental conditions	1850:1891	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	2	34	theme	heterogeneous	292:304	arg1	structure					306:314	the asymmetric and heterogeneous structure	273:314	the asymmetric and heterogeneous structure of the Gram-negative OM	273:338	However, the asymmetric and heterogeneous structure of the Gram-negative OM poses unique challenges to the cell's successful adaption to rapid environmental transitions.
27795394	8	35	theme	species	1803:1809	arg1	loss					1781:1784	the selective loss	1767:1784	the selective loss of palmitoylated species	1767:1809	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	9	36	from	OM	1989:1990	arg1	response					1995:2002	response	1995:2002	response to various environmental stimuli	1995:2035	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	9	36	from	OM	1989:1990	arg1	present					1972:1978	present	1972:1978	present	1972:1978	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	10	37	from	dynamics	2080:2087	arg1	Typhimurium					2138:2148	Salmonella enterica serovar Typhimurium	2110:2148	Salmonella enterica serovar Typhimurium	2110:2148	We developed a system to track the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium following an environmental shift to PhoP/Q- and PmrA/B-inducing conditions.
27795394	2	38	theme	environmental	407:419	arg1	transitions					421:431	rapid environmental transitions	401:431	rapid environmental transitions	401:431	However, the asymmetric and heterogeneous structure of the Gram-negative OM poses unique challenges to the cell's successful adaption to rapid environmental transitions.
27795394	0	39	theme	Environmental	113:125	arg1	Transitions					127:137	Environmental Transitions	113:137	Environmental Transitions	113:137	Outer Membrane Vesicle Production Facilitates LPS Remodeling and Outer Membrane Maintenance in Salmonella during Environmental Transitions.
27795394	9	40	dep	IMPORTANCE	1894:1903	arg1	bacteria					1923:1930	All Gram-negative bacteria	1905:1930	IMPORTANCE All Gram-negative bacteria	1894:1930	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	5	41	theme	PhoP/Q	1021:1026	arg1	activation					1007:1016	activation	1007:1016	activation of PhoP/Q and PmrA/B two-component systems (TCSs)	1007:1066	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	6	42	theme	toxic	1095:1099	arg1	metals					1101:1106	toxic metals	1095:1106	toxic metals	1095:1106	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	0	43	theme	Outer	0:4	arg1	Production					23:32	Outer Membrane Vesicle Production	0:32	Outer Membrane Vesicle Production	0:32	Outer Membrane Vesicle Production Facilitates LPS Remodeling and Outer Membrane Maintenance in Salmonella during Environmental Transitions.
27795394	5	44	theme	well-characterized	971:988	arg1	system					996:1001	a well-characterized model system	969:1001	a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs)	969:1066	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	5	44	theme	well-characterized	971:988	arg1	enterica					959:966	Salmonella enterica	948:966	Salmonella enterica	948:966	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	7	45	theme	PmrA/B-activating	1307:1323	arg1	conditions					1325:1334	PhoP/Q- and PmrA/B-activating conditions	1295:1334	PhoP/Q- and PmrA/B-activating conditions	1295:1334	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	12	46	theme	selection	2409:2417	arg1	model					2392:2396	a stochastic model	2379:2396	a stochastic model of content selection	2379:2417	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	8	47	theme	relative	1534:1541	arg1	abundance					1543:1551	the relative abundance	1530:1551	the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts	1530:1663	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	10	48	from	change	2100:2105	arg1	Typhimurium					2138:2148	Salmonella enterica serovar Typhimurium	2110:2148	Salmonella enterica serovar Typhimurium	2110:2148	We developed a system to track the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium following an environmental shift to PhoP/Q- and PmrA/B-inducing conditions.
27795394	0	49	theme	Vesicle	15:21	arg1	Production					23:32	Outer Membrane Vesicle Production	0:32	Outer Membrane Vesicle Production	0:32	Outer Membrane Vesicle Production Facilitates LPS Remodeling and Outer Membrane Maintenance in Salmonella during Environmental Transitions.
27795394	7	50	theme	PhoP/Q-	1295:1301	arg1	conditions					1325:1334	PhoP/Q- and PmrA/B-activating conditions	1295:1334	PhoP/Q- and PmrA/B-activating conditions	1295:1334	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	12	51	theme	covalent	2486:2493	arg1	modifications					2495:2507	covalent modifications	2486:2507	covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions	2486:2579	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	9	52	from	present	1972:1978	arg1	OM					1989:1990	their OM	1983:1990	their OM in response to various environmental stimuli	1983:2035	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	14	53	from	role	2753:2756	arg1	remodeling					2785:2794	OM remodeling	2782:2794	OM remodeling	2782:2794	These results highlight a role for the OMV response in OM remodeling and maintenance processes in Gram-negative bacteria.
27795394	14	53	from	role	2753:2756	arg1	processes					2812:2820	maintenance processes	2800:2820	maintenance processes	2800:2820	These results highlight a role for the OMV response in OM remodeling and maintenance processes in Gram-negative bacteria.
27795394	3	54	theme	OM	477:478	arg1	material					493:500	OM phospholipid material	477:500	OM phospholipid material	477:500	Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth.
27795394	8	55	theme	A	1562:1562	arg1	species					1564:1570	lipid A species	1556:1570	lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts	1556:1663	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	14	56	from	processes	2812:2820	arg1	bacteria					2839:2846	Gram-negative bacteria	2825:2846	Gram-negative bacteria	2825:2846	These results highlight a role for the OMV response in OM remodeling and maintenance processes in Gram-negative bacteria.
27795394	7	57	theme	OMV	1502:1504	arg1	diameter					1506:1513	increased OMV diameter	1492:1513	increased OMV diameter	1492:1513	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	12	58	theme	species	2467:2473	arg1	retention					2446:2454	the significant retention	2430:2454	the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions	2430:2579	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	8	59	theme	present	1572:1578	arg1	species					1564:1570	lipid A species	1556:1570	lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts	1556:1663	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	2	60	theme	unique	346:351	arg1	challenges					353:362	unique challenges	346:362	unique challenges to the cell's successful adaption to rapid environmental transitions	346:431	However, the asymmetric and heterogeneous structure of the Gram-negative OM poses unique challenges to the cell's successful adaption to rapid environmental transitions.
27795394	8	61	attach	present	1572:1578	arg2	species					1564:1570	lipid A species	1556:1570	lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts	1556:1663	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	8	61	attach	present	1572:1578	arg1	OMVs					1611:1614	the OM and the newly budded OMVs	1583:1614	OMVs	1611:1614	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	8	61	attach	present	1572:1578	arg1	OM					1587:1588	the OM and the newly budded OMVs	1583:1614	OM	1587:1588	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	5	62	theme	PmrA/B	1032:1037	arg1	TCSs					1062:1065	TCSs	1062:1065	TCSs	1062:1065	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	5	62	theme	PmrA/B	1032:1037	arg1	systems					1053:1059	PmrA/B two-component systems	1032:1059	PmrA/B two-component systems (TCSs)	1032:1066	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	4	63	dep	way	770:772	arg1	remove					789:794	remove	789:794	to selectively remove environmentally disadvantageous LPS species	774:838	As all Gram-negative bacteria constitutively shed OM vesicles (OMVs), we propose that cells may utilize OMV formation as a way to selectively remove environmentally disadvantageous LPS species.
27795394	4	64	theme	disadvantageous	812:826	arg1	species					832:838	environmentally disadvantageous LPS species	796:838	environmentally disadvantageous LPS species	796:838	As all Gram-negative bacteria constitutively shed OM vesicles (OMVs), we propose that cells may utilize OMV formation as a way to selectively remove environmentally disadvantageous LPS species.
27795394	2	65	theme	successful	378:387	arg1	adaption					389:396	the cell's successful adaption	367:396	the cell's successful adaption to rapid environmental transitions	367:431	However, the asymmetric and heterogeneous structure of the Gram-negative OM poses unique challenges to the cell's successful adaption to rapid environmental transitions.
27795394	1	66	theme	outer	200:204	arg1	membrane					206:213	outer membrane	200:213	outer membrane (OM) composition	200:230	The ability of Gram-negative bacteria to carefully modulate outer membrane (OM) composition is essential to their survival.
27795394	1	66	theme	outer	200:204	arg1	OM					216:217	OM	216:217	OM	216:217	The ability of Gram-negative bacteria to carefully modulate outer membrane (OM) composition is essential to their survival.
27795394	5	67	theme	systems	1053:1059	arg1	activation					1007:1016	activation	1007:1016	activation of PhoP/Q and PmrA/B two-component systems (TCSs)	1007:1066	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	10	68	theme	change	2100:2105	arg1	dynamics					2080:2087	the native dynamics	2069:2087	the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium	2069:2148	We developed a system to track the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium following an environmental shift to PhoP/Q- and PmrA/B-inducing conditions.
27795394	12	69	theme	content	2401:2407	arg1	selection					2409:2417	content selection	2401:2417	content selection	2401:2417	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	3	70	theme	known	521:525	arg1	mechanism					527:535	no known mechanism	518:535	no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms	518:602	Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth.
27795394	8	71	theme	environmental	1644:1656	arg1	shifts					1658:1663	rapid environmental shifts	1638:1663	rapid environmental shifts	1638:1663	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	7	72	theme	greater	1464:1470	arg1	production					1476:1485	greater OMV production	1464:1485	greater OMV production	1464:1485	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	10	73	theme	lipid	2092:2096	arg1	A					2098:2098	lipid A	2092:2098	lipid A change in Salmonella enterica serovar Typhimurium	2092:2148	We developed a system to track the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium following an environmental shift to PhoP/Q- and PmrA/B-inducing conditions.
27795394	12	74	theme	OMVs	2361:2364	arg1	content					2350:2356	the lipid A content	2338:2356	the lipid A content of OMVs	2338:2364	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	8	75	theme	environmental	1868:1880	arg1	conditions					1882:1891	moderately acidic environmental conditions	1850:1891	moderately acidic environmental conditions	1850:1891	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	3	76	theme	unfavorable	556:566	arg1	glycoforms					593:602	unfavorable lipopolysaccharide (LPS) glycoforms	556:602	unfavorable lipopolysaccharide (LPS) glycoforms	556:602	Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth.
27795394	10	77	theme	PmrA/B-inducing	2198:2212	arg1	conditions					2214:2223	PhoP/Q- and PmrA/B-inducing conditions	2186:2223	PhoP/Q- and PmrA/B-inducing conditions	2186:2223	We developed a system to track the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium following an environmental shift to PhoP/Q- and PmrA/B-inducing conditions.
27795394	7	78	theme	LPS	1459:1461	arg1	species					1448:1454	previously dominant species	1428:1454	previously dominant species of LPS	1428:1461	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	7	79	theme	dominant	1439:1446	arg1	species					1448:1454	previously dominant species	1428:1454	previously dominant species of LPS	1428:1461	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	9	80	theme	Gram-negative	1909:1921	arg1	bacteria					1923:1930	All Gram-negative bacteria	1905:1930	IMPORTANCE All Gram-negative bacteria	1894:1930	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	6	81	theme	specific	1246:1253	arg1	proteins					1258:1265	specific OM proteins	1246:1265	specific OM proteins	1246:1265	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	1	82	theme	membrane	206:213	arg1	composition					220:230	outer membrane (OM) composition	200:230	outer membrane (OM) composition	200:230	The ability of Gram-negative bacteria to carefully modulate outer membrane (OM) composition is essential to their survival.
27795394	7	83	theme	species	1448:1454	arg1	OM					1406:1407	the OM	1402:1407	the OM	1402:1407	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	7	83	theme	species	1448:1454	arg1	diameter					1506:1513	increased OMV diameter	1492:1513	increased OMV diameter	1492:1513	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	7	83	theme	species	1448:1454	arg1	production					1476:1485	greater OMV production	1464:1485	greater OMV production	1464:1485	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	7	83	theme	species	1448:1454	arg1	downregulation					1410:1423	downregulation	1410:1423	downregulation of previously dominant species of LPS	1410:1461	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	2	84	theme	OM	337:338	arg1	structure					306:314	the asymmetric and heterogeneous structure	273:314	the asymmetric and heterogeneous structure of the Gram-negative OM	273:338	However, the asymmetric and heterogeneous structure of the Gram-negative OM poses unique challenges to the cell's successful adaption to rapid environmental transitions.
27795394	8	85	theme	lipid	1691:1695	arg1	species					1699:1705	lipid A species	1691:1705	lipid A species	1691:1705	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	5	86	theme	composition	879:889	arg1	kinetics					864:871	the native kinetics	853:871	the native kinetics of OM composition	853:889	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	9	87	theme	structural	1942:1951	arg1	composition					1953:1963	the structural composition	1938:1963	the structural composition of LPS present in their OM in response to various environmental stimuli	1938:2035	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	4	88	theme	OM	697:698	arg1	OMVs					710:713	OMVs	710:713	OMVs	710:713	As all Gram-negative bacteria constitutively shed OM vesicles (OMVs), we propose that cells may utilize OMV formation as a way to selectively remove environmentally disadvantageous LPS species.
27795394	4	88	theme	OM	697:698	arg1	vesicles					700:707	OM vesicles	697:707	OM vesicles (OMVs)	697:714	As all Gram-negative bacteria constitutively shed OM vesicles (OMVs), we propose that cells may utilize OMV formation as a way to selectively remove environmentally disadvantageous LPS species.
27795394	9	89	attach	present	1972:1978	arg2	composition					1953:1963	the structural composition	1938:1963	the structural composition of LPS present in their OM in response to various environmental stimuli	1938:2035	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	9	89	attach	present	1972:1978	arg1	OM					1989:1990	their OM	1983:1990	their OM in response to various environmental stimuli	1983:2035	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	8	90	theme	species	1699:1705	arg1	retention					1678:1686	the retention	1674:1686	the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species	1674:1809	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	12	91	theme	A	2348:2348	arg1	content					2350:2356	the lipid A content	2338:2356	the lipid A content of OMVs	2338:2364	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	6	92	theme	cations	1154:1160	arg1	peptides					1123:1130	antimicrobial peptides	1109:1130	antimicrobial peptides	1109:1130	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	6	92	theme	cations	1154:1160	arg1	lack					1137:1140	lack	1137:1140	lack of divalent cations	1137:1160	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	6	92	theme	cations	1154:1160	arg1	pH					1091:1092	acidic pH	1084:1092	acidic pH	1084:1092	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	6	92	theme	cations	1154:1160	arg1	metals					1101:1106	toxic metals	1095:1106	toxic metals	1095:1106	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	11	93	theme	A	2299:2299	arg1	content					2301:2307	lipid A content	2293:2307	lipid A content	2293:2307	We show that growth conditions influence OMV production, size, and lipid A content.
27795394	10	94	theme	environmental	2163:2175	arg1	shift					2177:2181	an environmental shift	2160:2181	an environmental shift to PhoP/Q- and PmrA/B-inducing conditions	2160:2223	We developed a system to track the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium following an environmental shift to PhoP/Q- and PmrA/B-inducing conditions.
27795394	8	95	from	OM	1587:1588	arg1	present					1572:1578	present	1572:1578	present	1572:1578	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	3	96	theme	slow	612:615	arg1	dilution					617:624	slow dilution	612:624	slow dilution	612:624	Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth.
27795394	8	97	theme	modified	1712:1719	arg1	moieties					1731:1738	modified phosphate moieties	1712:1738	modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species	1712:1809	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	5	98	theme	native	857:862	arg1	kinetics					864:871	the native kinetics	853:871	the native kinetics of OM composition	853:889	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	6	99	theme	O	1220:1220	arg1	antigen					1222:1228	the O antigen	1216:1228	the O antigen	1216:1228	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	9	100	theme	present	1972:1978	arg1	composition					1953:1963	the structural composition	1938:1963	the structural composition of LPS present in their OM in response to various environmental stimuli	1938:2035	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	4	101	theme	Gram-negative	654:666	arg1	bacteria					668:675	all Gram-negative bacteria	650:675	all Gram-negative bacteria	650:675	As all Gram-negative bacteria constitutively shed OM vesicles (OMVs), we propose that cells may utilize OMV formation as a way to selectively remove environmentally disadvantageous LPS species.
27795394	6	102	theme	LPS	1185:1187	arg1	A					1195:1195	the LPS lipid A	1181:1195	the LPS lipid A	1181:1195	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	7	103	theme	species	1384:1390	arg1	addition					1363:1370	the addition	1359:1370	the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter	1359:1513	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	0	104	theme	Membrane	71:78	arg1	Maintenance					80:90	Outer Membrane Maintenance	65:90	Outer Membrane Maintenance	65:90	Outer Membrane Vesicle Production Facilitates LPS Remodeling and Outer Membrane Maintenance in Salmonella during Environmental Transitions.
27795394	8	105	theme	concomitant	1750:1760	arg1	OM					1747:1748	the OM	1743:1748	the OM concomitant with the selective loss of palmitoylated species	1743:1809	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	1	106	theme	Gram-negative	155:167	arg1	bacteria					169:176	Gram-negative bacteria	155:176	Gram-negative bacteria	155:176	The ability of Gram-negative bacteria to carefully modulate outer membrane (OM) composition is essential to their survival.
27795394	14	107	theme	OM	2782:2783	arg1	remodeling					2785:2794	OM remodeling	2782:2794	OM remodeling	2782:2794	These results highlight a role for the OMV response in OM remodeling and maintenance processes in Gram-negative bacteria.
27795394	12	108	theme	A	2465:2465	arg1	species					2467:2473	lipid A species	2459:2473	lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions	2459:2579	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	5	109	theme	environmental	923:935	arg1	changes					937:943	physiologically relevant environmental changes	898:943	physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs)	898:1066	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	9	110	theme	environmental	2015:2027	arg1	stimuli					2029:2035	various environmental stimuli	2007:2035	various environmental stimuli	2007:2035	IMPORTANCE All Gram-negative bacteria alter the structural composition of LPS present in their OM in response to various environmental stimuli.
27795394	5	111	from	changes	937:943	arg1	system					996:1001	a well-characterized model system	969:1001	a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs)	969:1066	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	5	111	from	changes	937:943	arg1	enterica					959:966	Salmonella enterica	948:966	Salmonella enterica	948:966	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	8	112	with	retention	1678:1686	arg1	moieties					1731:1738	modified phosphate moieties	1712:1738	modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species	1712:1809	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	13	113	theme	incorporation	2702:2714	arg1	likelihood					2684:2693	the likelihood	2680:2693	the likelihood of its incorporation into OMVs	2680:2724	Furthermore, palmitoylation of the lipid A to form hepta-acylated species substantially increases the likelihood of its incorporation into OMVs.
27795394	13	114	theme	hepta-acylated	2633:2646	arg1	species					2648:2654	hepta-acylated species	2633:2654	hepta-acylated species	2633:2654	Furthermore, palmitoylation of the lipid A to form hepta-acylated species substantially increases the likelihood of its incorporation into OMVs.
27795394	8	115	theme	palmitoylated	1789:1801	arg1	species					1803:1809	palmitoylated species	1789:1809	palmitoylated species	1789:1809	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	11	116	theme	OMV	2267:2269	arg1	production					2271:2280	OMV production	2267:2280	OMV production	2267:2280	We show that growth conditions influence OMV production, size, and lipid A content.
27795394	2	117	theme	rapid	401:405	arg1	transitions					421:431	rapid environmental transitions	401:431	rapid environmental transitions	401:431	However, the asymmetric and heterogeneous structure of the Gram-negative OM poses unique challenges to the cell's successful adaption to rapid environmental transitions.
27795394	0	118	theme	Membrane	6:13	arg1	Production					23:32	Outer Membrane Vesicle Production	0:32	Outer Membrane Vesicle Production	0:32	Outer Membrane Vesicle Production Facilitates LPS Remodeling and Outer Membrane Maintenance in Salmonella during Environmental Transitions.
27795394	14	119	theme	OMV	2766:2768	arg1	response					2770:2777	the OMV response	2762:2777	the OMV response	2762:2777	These results highlight a role for the OMV response in OM remodeling and maintenance processes in Gram-negative bacteria.
27795394	8	120	theme	abundance	1543:1551	arg1	Comparison					1516:1525	Comparison	1516:1525	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts	1516:1663	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	3	121	dep	mechanisms	443:452	arg1	degrade					469:475	degrade	469:475	degrade OM phospholipid material	469:500	Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth.
27795394	3	121	dep	mechanisms	443:452	arg1	recycle					457:463	recycle	457:463	recycle	457:463	Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth.
27795394	6	122	dep	pH	1091:1092	arg1	response					1072:1079	response	1072:1079	response	1072:1079	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	13	123	theme	lipid	2617:2621	arg1	A					2623:2623	the lipid A	2613:2623	the lipid A	2613:2623	Furthermore, palmitoylation of the lipid A to form hepta-acylated species substantially increases the likelihood of its incorporation into OMVs.
27795394	5	124	theme	model	990:994	arg1	system					996:1001	a well-characterized model system	969:1001	a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs)	969:1066	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	5	124	theme	model	990:994	arg1	enterica					959:966	Salmonella enterica	948:966	Salmonella enterica	948:966	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	6	125	theme	acidic	1084:1089	arg1	pH					1091:1092	acidic pH	1084:1092	acidic pH	1084:1092	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	8	126	theme	lipid	1556:1560	arg1	species					1564:1570	lipid A species	1556:1570	lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts	1556:1663	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	13	127	theme	A	2623:2623	arg1	palmitoylation					2595:2608	palmitoylation	2595:2608	palmitoylation of the lipid A to form hepta-acylated species	2595:2654	Furthermore, palmitoylation of the lipid A to form hepta-acylated species substantially increases the likelihood of its incorporation into OMVs.
27795394	4	128	theme	OMV	751:753	arg1	formation					755:763	OMV formation	751:763	OMV formation	751:763	As all Gram-negative bacteria constitutively shed OM vesicles (OMVs), we propose that cells may utilize OMV formation as a way to selectively remove environmentally disadvantageous LPS species.
27795394	4	128	theme	OMV	751:753	arg1	way					770:772	a way	768:772	a way to selectively remove environmentally disadvantageous LPS species	768:838	As all Gram-negative bacteria constitutively shed OM vesicles (OMVs), we propose that cells may utilize OMV formation as a way to selectively remove environmentally disadvantageous LPS species.
27795394	0	129	theme	LPS	46:48	arg1	Remodeling					50:59	LPS Remodeling	46:59	LPS Remodeling	46:59	Outer Membrane Vesicle Production Facilitates LPS Remodeling and Outer Membrane Maintenance in Salmonella during Environmental Transitions.
27795394	7	130	theme	environmental	1271:1283	arg1	change					1285:1290	An environmental change	1268:1290	An environmental change to PhoP/Q- and PmrA/B-activating conditions	1268:1334	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	8	131	theme	species	1564:1570	arg1	abundance					1543:1551	the relative abundance	1530:1551	the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts	1530:1663	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	4	132	theme	LPS	828:830	arg1	species					832:838	environmentally disadvantageous LPS species	796:838	environmentally disadvantageous LPS species	796:838	As all Gram-negative bacteria constitutively shed OM vesicles (OMVs), we propose that cells may utilize OMV formation as a way to selectively remove environmentally disadvantageous LPS species.
27795394	3	133	theme	phospholipid	480:491	arg1	material					493:500	OM phospholipid material	477:500	OM phospholipid material	477:500	Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth.
27795394	6	134	theme	divalent	1145:1152	arg1	cations					1154:1160	divalent cations	1145:1160	divalent cations	1145:1160	In response to acidic pH, toxic metals, antimicrobial peptides, and lack of divalent cations, these TCSs modify the LPS lipid A and core, lengthen the O antigen, and upregulate specific OM proteins.
27795394	1	135	theme	bacteria	169:176	arg1	ability					144:150	The ability	140:150	The ability of Gram-negative bacteria to carefully modulate outer membrane (OM) composition	140:230	The ability of Gram-negative bacteria to carefully modulate outer membrane (OM) composition is essential to their survival.
27795394	1	135	theme	bacteria	169:176	arg1	essential					235:243	essential	235:243	essential	235:243	The ability of Gram-negative bacteria to carefully modulate outer membrane (OM) composition is essential to their survival.
27795394	12	136	theme	4'-phosphate	2532:2543	arg1	moieties					2545:2552	their 1- and 4'-phosphate moieties	2519:2552	their 1- and 4'-phosphate moieties	2519:2552	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	7	137	theme	LPS	1395:1397	arg1	species					1384:1390	modified species	1375:1390	modified species of LPS	1375:1397	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	10	138	theme	native	2073:2078	arg1	dynamics					2080:2087	the native dynamics	2069:2087	the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium	2069:2148	We developed a system to track the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium following an environmental shift to PhoP/Q- and PmrA/B-inducing conditions.
27795394	12	139	theme	stochastic	2381:2390	arg1	model					2392:2396	a stochastic model	2379:2396	a stochastic model of content selection	2379:2417	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	10	140	from	Typhimurium	2138:2148	arg1	dynamics					2080:2087	the native dynamics	2069:2087	the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium	2069:2148	We developed a system to track the native dynamics of lipid A change in Salmonella enterica serovar Typhimurium following an environmental shift to PhoP/Q- and PmrA/B-inducing conditions.
27795394	14	141	from	remodeling	2785:2794	arg1	bacteria					2839:2846	Gram-negative bacteria	2825:2846	Gram-negative bacteria	2825:2846	These results highlight a role for the OMV response in OM remodeling and maintenance processes in Gram-negative bacteria.
27795394	8	142	theme	rapid	1638:1642	arg1	shifts					1658:1663	rapid environmental shifts	1638:1663	rapid environmental shifts	1638:1663	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	7	143	theme	increased	1492:1500	arg1	diameter					1506:1513	increased OMV diameter	1492:1513	increased OMV diameter	1492:1513	An environmental change to PhoP/Q- and PmrA/B-activating conditions simultaneously induced the addition of modified species of LPS to the OM, downregulation of previously dominant species of LPS, greater OMV production, and increased OMV diameter.
27795394	5	144	theme	two-component	1039:1051	arg1	TCSs					1062:1065	TCSs	1062:1065	TCSs	1062:1065	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	5	144	theme	two-component	1039:1051	arg1	systems					1053:1059	PmrA/B two-component systems	1032:1059	PmrA/B two-component systems (TCSs)	1032:1066	We examined the native kinetics of OM composition during physiologically relevant environmental changes in Salmonella enterica, a well-characterized model system for activation of PhoP/Q and PmrA/B two-component systems (TCSs).
27795394	12	145	theme	1-	2525:2526	arg1	moieties					2545:2552	their 1- and 4'-phosphate moieties	2519:2552	their 1- and 4'-phosphate moieties	2519:2552	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	12	146	theme	host-like	2560:2568	arg1	conditions					2570:2579	host-like conditions	2560:2579	host-like conditions	2560:2579	We further demonstrate that the lipid A content of OMVs does not fit a stochastic model of content selection, revealing the significant retention of lipid A species containing covalent modifications that mask their 1- and 4'-phosphate moieties under host-like conditions.
27795394	8	147	theme	shifts	1658:1663	arg1	shifts					1658:1663	rapid environmental shifts	1638:1663	rapid environmental shifts	1638:1663	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
27795394	8	147	theme	shifts	1658:1663	arg1	sets					1630:1633	two sets	1626:1633	two sets of rapid environmental shifts	1626:1663	Comparison of the relative abundance of lipid A species present in the OM and the newly budded OMVs following two sets of rapid environmental shifts revealed the retention of lipid A species with modified phosphate moieties in the OM concomitant with the selective loss of palmitoylated species via vesiculation following exposure to moderately acidic environmental conditions.
25061728	4	0	theme	processes	753:761	arg1	activity					711:718	the activity	707:718	the activity of lipid- and protein-associated processes	707:761	Indeed, we observed that leukocytes, most notably, tune the activity of lipid- and protein-associated processes at the transcriptional level in accordance with the fluctuations in metabolite compositions of surrounding plasma.
25061728	4	1	theme	protein-associated	734:751	arg1	processes					753:761	lipid- and protein-associated processes	723:761	lipid- and protein-associated processes	723:761	Indeed, we observed that leukocytes, most notably, tune the activity of lipid- and protein-associated processes at the transcriptional level in accordance with the fluctuations in metabolite compositions of surrounding plasma.
25061728	5	2	from	dysfunction	1067:1077	arg1	production					1100:1109	energy production	1093:1109	energy production observed during inflammatory processes	1093:1148	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	5	3	from	alterations	962:972	arg1	bioenergetics					977:989	bioenergetics	977:989	bioenergetics	977:989	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	5	3	from	alterations	962:972	arg1	defenses					995:1002	defenses	995:1002	defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes	995:1148	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	2	4	theme	plasma	298:303	arg1	compositions					316:327	plasma metabolite compositions	298:327	plasma metabolite compositions	298:327	We characterized the interplay between plasma metabolite compositions and transcriptional response of leukocytes through integration of transcriptomics with plasma metabonomics.
25061728	5	5	theme	mitochondrial	1053:1065	arg1	dysfunction					1067:1077	mitochondrial dysfunction	1053:1077	mitochondrial dysfunction	1053:1077	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	3	6	theme	initial	621:627	arg1	stimuli					642:648	the initial inflammatory stimuli	617:648	the initial inflammatory stimuli	617:648	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
25061728	3	7	contain	have	531:534	arg1	changes					470:476	the drastic changes	458:476	the drastic changes in the immediate environment of the leukocytes	458:523	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
25061728	3	7	contain	have	531:534	arg2	effect					548:553	an adaptive effect	536:553	an adaptive effect	536:553	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
25061728	3	8	from	shaping	558:564	arg1	conjunction					600:610	conjunction	600:610	conjunction with the initial inflammatory stimuli	600:648	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
25061728	4	9	theme	transcriptional	770:784	arg1	level					786:790	the transcriptional level	766:790	the transcriptional level in accordance with the fluctuations in metabolite compositions of surrounding plasma	766:875	Indeed, we observed that leukocytes, most notably, tune the activity of lipid- and protein-associated processes at the transcriptional level in accordance with the fluctuations in metabolite compositions of surrounding plasma.
25061728	5	10	theme	transcriptional	901:915	arg1	control					917:923	the transcriptional control	897:923	the transcriptional control of only metabolic pathways	897:950	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	4	11	from	fluctuations	815:826	arg1	compositions					842:853	metabolite compositions	831:853	metabolite compositions of surrounding plasma	831:875	Indeed, we observed that leukocytes, most notably, tune the activity of lipid- and protein-associated processes at the transcriptional level in accordance with the fluctuations in metabolite compositions of surrounding plasma.
25061728	3	12	theme	transcriptional	572:586	arg1	response					588:595	their transcriptional response	566:595	their transcriptional response	566:595	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
25061728	1	13	theme	-omics	161:166	arg1	analyses					168:175	-omics analyses	161:175	-omics analyses	161:175	In this meta-study, we aimed to integrate biological insights gained from two levels of -omics analyses on the response to systemic inflammation induced by lipopolysaccharide in humans.
25061728	3	14	with	conjunction	600:610	arg1	stimuli					642:648	the initial inflammatory stimuli	617:648	the initial inflammatory stimuli	617:648	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
25061728	4	15	theme	metabolite	831:840	arg1	compositions					842:853	metabolite compositions	831:853	metabolite compositions of surrounding plasma	831:875	Indeed, we observed that leukocytes, most notably, tune the activity of lipid- and protein-associated processes at the transcriptional level in accordance with the fluctuations in metabolite compositions of surrounding plasma.
25061728	1	16	theme	analyses	168:175	arg1	levels					151:156	two levels	147:156	two levels of -omics analyses	147:175	In this meta-study, we aimed to integrate biological insights gained from two levels of -omics analyses on the response to systemic inflammation induced by lipopolysaccharide in humans.
25061728	0	17	theme	transcriptional	11:25	arg1	profiling					41:49	transcriptional and metabolic profiling	11:49	transcriptional and metabolic profiling	11:49	Integrated transcriptional and metabolic profiling in human endotoxemia.
25061728	2	18	theme	transcriptional	333:347	arg1	response					349:356	transcriptional response	333:356	transcriptional response	333:356	We characterized the interplay between plasma metabolite compositions and transcriptional response of leukocytes through integration of transcriptomics with plasma metabonomics.
25061728	5	19	theme	pathways	943:950	arg1	control					917:923	the transcriptional control	897:923	the transcriptional control of only metabolic pathways	897:950	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	5	20	theme	closer	880:885	arg1	look					887:890	A closer look	878:890	A closer look into the transcriptional control of only metabolic pathways	878:950	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	3	21	theme	inflammatory	629:640	arg1	stimuli					642:648	the initial inflammatory stimuli	617:648	the initial inflammatory stimuli	617:648	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
25061728	2	22	theme	plasma	416:421	arg1	metabonomics					423:434	plasma metabonomics	416:434	plasma metabonomics	416:434	We characterized the interplay between plasma metabolite compositions and transcriptional response of leukocytes through integration of transcriptomics with plasma metabonomics.
25061728	0	23	theme	metabolic	31:39	arg1	profiling					41:49	transcriptional and metabolic profiling	11:49	transcriptional and metabolic profiling	11:49	Integrated transcriptional and metabolic profiling in human endotoxemia.
25061728	2	24	theme	metabolite	305:314	arg1	compositions					316:327	plasma metabolite compositions	298:327	plasma metabolite compositions	298:327	We characterized the interplay between plasma metabolite compositions and transcriptional response of leukocytes through integration of transcriptomics with plasma metabonomics.
25061728	3	25	theme	leukocytes	514:523	arg1	environment					495:505	the immediate environment	481:505	the immediate environment of the leukocytes	481:523	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
25061728	2	26	theme	leukocytes	361:370	arg1	interplay					280:288	the interplay	276:288	the interplay between plasma metabolite compositions and transcriptional response of leukocytes	276:370	We characterized the interplay between plasma metabolite compositions and transcriptional response of leukocytes through integration of transcriptomics with plasma metabonomics.
25061728	5	27	theme	inflammatory	1127:1138	arg1	processes					1140:1148	inflammatory processes	1127:1148	inflammatory processes	1127:1148	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	1	28	theme	systemic	196:203	arg1	inflammation					205:216	systemic inflammation	196:216	systemic inflammation induced by lipopolysaccharide in humans	196:256	In this meta-study, we aimed to integrate biological insights gained from two levels of -omics analyses on the response to systemic inflammation induced by lipopolysaccharide in humans.
25061728	3	29	theme	drastic	462:468	arg1	changes					470:476	the drastic changes	458:476	the drastic changes in the immediate environment of the leukocytes	458:523	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
25061728	5	30	theme	metabolic	933:941	arg1	pathways					943:950	only metabolic pathways	928:950	only metabolic pathways	928:950	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	1	31	from	lipopolysaccharide	229:246	arg1	humans					251:256	humans	251:256	humans	251:256	In this meta-study, we aimed to integrate biological insights gained from two levels of -omics analyses on the response to systemic inflammation induced by lipopolysaccharide in humans.
25061728	0	32	theme	human	54:58	arg1	endotoxemia					60:70	human endotoxemia	54:70	human endotoxemia	54:70	Integrated transcriptional and metabolic profiling in human endotoxemia.
25061728	1	33	theme	biological	115:124	arg1	insights					126:133	biological insights	115:133	biological insights gained from two levels of -omics analyses on the response to systemic inflammation induced by lipopolysaccharide in humans	115:256	In this meta-study, we aimed to integrate biological insights gained from two levels of -omics analyses on the response to systemic inflammation induced by lipopolysaccharide in humans.
25061728	5	34	theme	energy	1093:1098	arg1	production					1100:1109	energy production	1093:1109	energy production observed during inflammatory processes	1093:1148	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	4	35	theme	surrounding	858:868	arg1	plasma					870:875	surrounding plasma	858:875	surrounding plasma	858:875	Indeed, we observed that leukocytes, most notably, tune the activity of lipid- and protein-associated processes at the transcriptional level in accordance with the fluctuations in metabolite compositions of surrounding plasma.
25061728	5	36	theme	oxidative	1012:1020	arg1	stress					1022:1027	oxidative stress	1012:1027	oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes	1012:1148	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	3	37	from	changes	470:476	arg1	environment					495:505	the immediate environment	481:505	the immediate environment of the leukocytes	481:523	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
25061728	4	38	theme	lipid-	723:728	arg1	processes					753:761	lipid- and protein-associated processes	723:761	lipid- and protein-associated processes	723:761	Indeed, we observed that leukocytes, most notably, tune the activity of lipid- and protein-associated processes at the transcriptional level in accordance with the fluctuations in metabolite compositions of surrounding plasma.
25061728	2	39	with	transcriptomics	395:409	arg1	metabonomics					423:434	plasma metabonomics	416:434	plasma metabonomics	416:434	We characterized the interplay between plasma metabolite compositions and transcriptional response of leukocytes through integration of transcriptomics with plasma metabonomics.
25061728	5	40	from	shifts	1083:1088	arg1	production					1100:1109	energy production	1093:1109	energy production observed during inflammatory processes	1093:1148	A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
25061728	3	41	theme	immediate	485:493	arg1	environment					495:505	the immediate environment	481:505	the immediate environment of the leukocytes	481:523	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
25061728	4	42	theme	plasma	870:875	arg1	compositions					842:853	metabolite compositions	831:853	metabolite compositions of surrounding plasma	831:875	Indeed, we observed that leukocytes, most notably, tune the activity of lipid- and protein-associated processes at the transcriptional level in accordance with the fluctuations in metabolite compositions of surrounding plasma.
25061728	2	43	theme	transcriptomics	395:409	arg1	integration					380:390	integration	380:390	integration of transcriptomics with plasma metabonomics	380:434	We characterized the interplay between plasma metabolite compositions and transcriptional response of leukocytes through integration of transcriptomics with plasma metabonomics.
25061728	3	44	theme	adaptive	539:546	arg1	effect					548:553	an adaptive effect	536:553	an adaptive effect	536:553	We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli.
28321788	6	0	theme	ribosomal	1011:1019	arg1	RNA					1021:1023	16S ribosomal RNA	1007:1023	16S ribosomal RNA (rRNA) sequencing method	1007:1048	A. viridians isolates were further identified by API identification system and 16S ribosomal RNA (rRNA) sequencing method.
28321788	6	0	theme	ribosomal	1011:1019	arg1	rRNA					1026:1029	rRNA	1026:1029	rRNA	1026:1029	A. viridians isolates were further identified by API identification system and 16S ribosomal RNA (rRNA) sequencing method.
28321788	3	1	theme	daily	610:614	arg1	yield					621:625	daily milk yield	610:625	daily milk yield	610:625	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	13	2	dep	observed	1982:1989	arg1	whereas					2067:2073	whereas	2067:2073	whereas	2067:2073	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	1	3	dep	viridians	144:152	arg1	viridans					158:165	A. viridans	155:165	A. viridans	155:165	Aerococcus viridians (A. viridans), an environmental Gram-positive bacterium, has been documented to be associated with bovine mastitis.
28321788	4	4	theme	September	766:774	arg1	2013					776:779	September 2013	766:779	September 2013 to August 2014	766:794	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	3	5	theme	bovine	487:492	arg1	mastitis					494:501	bovine mastitis	487:501	bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow	487:660	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	12	6	theme	cows	1788:1791	arg1	higher					1811:1816	higher	1811:1816	higher	1811:1816	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	12	6	theme	cows	1788:1791	arg1	SCC					1756:1758	The average SCC	1744:1758	The average SCC of the A. viridans infected cows	1744:1791	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	3	7	from	composition	632:642	arg1	SCC					604:606	SCC	604:606	SCC	604:606	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	7	from	composition	632:642	arg1	counts					596:601	the somatic cell counts	579:601	the somatic cell counts (SCC)	579:607	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	7	from	composition	632:642	arg1	udders					568:573	udders	568:573	udders	568:573	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	2	8	theme	exact	283:287	arg1	role					289:292	its exact role	279:292	its exact role in bovine mastitis and the changes it	279:330	However, its exact role in bovine mastitis and the changes it brings about in milk characteristics are not yet known.
28321788	4	9	theme	subclinical	685:695	arg1	milk					716:719	subclinical mastitis composite milk	685:719	subclinical mastitis composite milk	685:719	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	12	10	theme	milk	1913:1916	arg1	yield					1918:1922	daily milk yield	1907:1922	daily milk yield	1907:1922	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	3	11	theme	cow	658:660	arg1	yield					621:625	daily milk yield	610:625	daily milk yield	610:625	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	11	theme	cow	658:660	arg1	composition					632:642	composition	632:642	composition of individual cow	632:660	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	11	theme	cow	658:660	arg1	existence					538:546	existence	538:546	existence of this pathogen in udders and the somatic cell counts (SCC)	538:607	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	4	12	from	China	838:842	arg1	located					818:824	located	818:824	located	818:824	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	1	13	theme	environmental	172:184	arg1	viridians					144:152	Aerococcus viridians	133:152	Aerococcus viridians (A. viridans)	133:166	Aerococcus viridians (A. viridans), an environmental Gram-positive bacterium, has been documented to be associated with bovine mastitis.
28321788	1	13	theme	environmental	172:184	arg1	bacterium					200:208	an environmental Gram-positive bacterium	169:208	an environmental Gram-positive bacterium	169:208	Aerococcus viridians (A. viridans), an environmental Gram-positive bacterium, has been documented to be associated with bovine mastitis.
28321788	8	14	theme	milk	1191:1194	arg1	yield					1196:1200	milk yield	1191:1200	milk yield	1191:1200	SCC, milk yield, and milk composition data were from monthly Dairy Herd Improvement (DHI) results.
28321788	12	15	theme	viridans	1770:1777	arg1	cows					1788:1791	the A. viridans infected cows	1763:1791	the A. viridans infected cows	1763:1791	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	4	16	theme	commercial	802:811	arg1	herd					813:816	a commercial herd	800:816	a commercial herd located in Beijing, China	800:842	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	0	17	from	mastitis	72:79	arg1	yield					109:113	yield	109:113	yield	109:113	Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
28321788	0	17	from	mastitis	72:79	arg1	composition					120:130	composition	120:130	composition	120:130	Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
28321788	0	17	from	mastitis	72:79	arg1	SCC					104:106	milk SCC	99:106	milk SCC	99:106	Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
28321788	9	18	with	samples	1420:1426	arg1	SCC					1438:1440	high SCC	1433:1440	high SCC	1433:1440	Results showed that a total of 279 (16.67%) A. viridans isolates were identified from among 1674 bacterial isolates cultured from milk samples with high SCC.
28321788	4	19	from	located	818:824	arg1	China					838:842	China	838:842	China	838:842	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	14	20	theme	milk	2349:2352	arg1	yield					2354:2358	milk yield	2349:2358	milk yield	2349:2358	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	4	21	theme	located	818:824	arg1	herd					813:816	a commercial herd	800:816	a commercial herd located in Beijing, China	800:842	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	8	22	theme	milk	1207:1210	arg1	composition					1212:1222	milk composition	1207:1222	milk composition	1207:1222	SCC, milk yield, and milk composition data were from monthly Dairy Herd Improvement (DHI) results.
28321788	10	23	theme	summer	1527:1532	arg1	season					1534:1539	the summer season	1523:1539	the summer season	1523:1539	The incidence of mastitis caused by A. viridans was the highest (48-53%) during the summer season.
28321788	1	24	theme	Aerococcus	133:142	arg1	viridians					144:152	Aerococcus viridians	133:152	Aerococcus viridians (A. viridans)	133:166	Aerococcus viridians (A. viridans), an environmental Gram-positive bacterium, has been documented to be associated with bovine mastitis.
28321788	1	24	theme	Aerococcus	133:142	arg1	bacterium					200:208	an environmental Gram-positive bacterium	169:208	an environmental Gram-positive bacterium	169:208	Aerococcus viridians (A. viridans), an environmental Gram-positive bacterium, has been documented to be associated with bovine mastitis.
28321788	13	25	located	observed	1982:1989	arg1	P > 0.05					2007:2014	P > 0.05	2007:2014	P > 0.05	2007:2014	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	13	25	located	observed	1982:1989	arg2	Reductions					1961:1970	Reductions	1961:1970	Reductions	1961:1970	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	13	25	located	observed	1982:1989	arg1	lactose					2018:2024	lactose	2018:2024	lactose (P < 0.01)	2018:2035	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	13	25	located	observed	1982:1989	arg1	solids					2048:2053	total solids	2042:2053	total solids (P > 0.05)	2042:2064	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	13	25	located	observed	1982:1989	arg1	P < 0.01					2027:2034	P < 0.01	2027:2034	P < 0.01	2027:2034	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	13	25	located	observed	1982:1989	arg1	content					1998:2004	fat content	1994:2004	fat content (P > 0.05)	1994:2015	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	13	25	located	observed	1982:1989	arg1	P > 0.05					2056:2063	P > 0.05	2056:2063	P > 0.05	2056:2063	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	13	26	theme	total	2042:2046	arg1	P > 0.05					2056:2063	P > 0.05	2056:2063	P > 0.05	2056:2063	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	13	26	theme	total	2042:2046	arg1	solids					2048:2053	total solids	2042:2053	total solids (P > 0.05)	2042:2064	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	2	27	from	role	289:292	arg1	mastitis					304:311	bovine mastitis	297:311	bovine mastitis	297:311	However, its exact role in bovine mastitis and the changes it brings about in milk characteristics are not yet known.
28321788	2	27	from	role	289:292	arg1	changes					321:327	the changes	317:327	the changes it	317:330	However, its exact role in bovine mastitis and the changes it brings about in milk characteristics are not yet known.
28321788	8	28	theme	Dairy	1247:1251	arg1	results					1276:1282	monthly Dairy Herd Improvement (DHI) results	1239:1282	monthly Dairy Herd Improvement (DHI) results	1239:1282	SCC, milk yield, and milk composition data were from monthly Dairy Herd Improvement (DHI) results.
28321788	12	29	theme	average	1748:1754	arg1	higher					1811:1816	higher	1811:1816	higher	1811:1816	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	12	29	theme	average	1748:1754	arg1	SCC					1756:1758	The average SCC	1744:1758	The average SCC of the A. viridans infected cows	1744:1791	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	14	30	theme	wherein	2313:2319	arg1	viridans					2219:2226	A. viridans	2216:2226	A. viridans	2216:2226	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	14	30	theme	wherein	2313:2319	arg1	agent					2276:2280	an emerging aetiological agent	2251:2280	an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition	2251:2375	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	4	31	theme	composite	706:714	arg1	milk					716:719	subclinical mastitis composite milk	685:719	subclinical mastitis composite milk	685:719	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	14	32	theme	subclinical	2292:2302	arg1	wherein					2313:2319	bovine subclinical mastitis wherein	2285:2319	bovine subclinical mastitis wherein	2285:2319	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	9	33	theme	high	1433:1436	arg1	SCC					1438:1440	high SCC	1433:1440	high SCC	1433:1440	Results showed that a total of 279 (16.67%) A. viridans isolates were identified from among 1674 bacterial isolates cultured from milk samples with high SCC.
28321788	8	34	theme	DHI	1271:1273	arg1	results					1276:1282	monthly Dairy Herd Improvement (DHI) results	1239:1282	monthly Dairy Herd Improvement (DHI) results	1239:1282	SCC, milk yield, and milk composition data were from monthly Dairy Herd Improvement (DHI) results.
28321788	1	35	theme	bovine	253:258	arg1	mastitis					260:267	bovine mastitis	253:267	bovine mastitis	253:267	Aerococcus viridians (A. viridans), an environmental Gram-positive bacterium, has been documented to be associated with bovine mastitis.
28321788	6	36	theme	API	977:979	arg1	system					996:1001	API identification system	977:1001	API identification system	977:1001	A. viridians isolates were further identified by API identification system and 16S ribosomal RNA (rRNA) sequencing method.
28321788	3	37	from	existence	538:546	arg1	SCC					604:606	SCC	604:606	SCC	604:606	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	37	from	existence	538:546	arg1	counts					596:601	the somatic cell counts	579:601	the somatic cell counts (SCC)	579:607	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	37	from	existence	538:546	arg1	udders					568:573	udders	568:573	udders	568:573	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	38	theme	study	418:422	arg1	objectives					392:401	The objectives	388:401	The objectives of the current study	388:422	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	0	39	theme	Aerococcus	19:28	arg1	viridans					30:37	Aerococcus viridans	19:37	Aerococcus viridans	19:37	Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
28321788	14	40	theme	mastitis	2304:2311	arg1	wherein					2313:2319	bovine subclinical mastitis wherein	2285:2319	bovine subclinical mastitis wherein	2285:2319	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	13	41	theme	protein	2075:2081	arg1	content					2083:2089	protein content	2075:2089	protein content	2075:2089	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	4	42	theme	monthly	744:750	arg1	data					756:759	monthly DHI data	744:759	monthly DHI data from September 2013 to August 2014	744:794	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	7	43	theme	diffusion	1068:1076	arg1	method					1078:1083	Kirby-Bauer disk diffusion method	1051:1083	Kirby-Bauer disk diffusion method	1051:1083	Kirby-Bauer disk diffusion method was used to test the antibiotic resistance of A. viridians against kinds of antimicrobial substance.
28321788	7	44	used	used	1089:1092	arg2	method					1078:1083	Kirby-Bauer disk diffusion method	1051:1083	Kirby-Bauer disk diffusion method	1051:1083	Kirby-Bauer disk diffusion method was used to test the antibiotic resistance of A. viridians against kinds of antimicrobial substance.
28321788	14	45	theme	bovine	2285:2290	arg1	wherein					2313:2319	bovine subclinical mastitis wherein	2285:2319	bovine subclinical mastitis wherein	2285:2319	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	13	46	theme	cows	2145:2148	arg1	samples					2134:2140	milk samples	2129:2140	milk samples of cows infected with A. viridans	2129:2174	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	3	47	theme	somatic	583:589	arg1	SCC					604:606	SCC	604:606	SCC	604:606	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	47	theme	somatic	583:589	arg1	counts					596:601	the somatic cell counts	579:601	the somatic cell counts (SCC)	579:607	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	13	48	dep	increased	2091:2099	arg1	P < 0.01					2116:2123	P < 0.01	2116:2123	P < 0.01	2116:2123	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	7	49	theme	Kirby-Bauer	1051:1061	arg1	method					1078:1083	Kirby-Bauer disk diffusion method	1051:1083	Kirby-Bauer disk diffusion method	1051:1083	Kirby-Bauer disk diffusion method was used to test the antibiotic resistance of A. viridians against kinds of antimicrobial substance.
28321788	6	50	theme	sequencing	1032:1041	arg1	method					1043:1048	16S ribosomal RNA (rRNA) sequencing method	1007:1048	16S ribosomal RNA (rRNA) sequencing method	1007:1048	A. viridians isolates were further identified by API identification system and 16S ribosomal RNA (rRNA) sequencing method.
28321788	2	51	theme	bovine	297:302	arg1	mastitis					304:311	bovine mastitis	297:311	bovine mastitis	297:311	However, its exact role in bovine mastitis and the changes it brings about in milk characteristics are not yet known.
28321788	14	52	theme	emerging	2254:2261	arg1	viridans					2219:2226	A. viridans	2216:2226	A. viridans	2216:2226	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	14	52	theme	emerging	2254:2261	arg1	agent					2276:2280	an emerging aetiological agent	2251:2280	an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition	2251:2375	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	11	53	theme	isolates	1558:1565	arg1	Majority					1542:1549	Majority	1542:1549	Majority of the isolates	1542:1565	Majority of the isolates were susceptible to most of antimicrobial compounds tested, especially to β-lactams, but were found to be resistant (50-90%) to aminoglycosides, sulfonamides, and tetracycline.
28321788	7	54	theme	substance	1175:1183	arg1	kinds					1152:1156	kinds	1152:1156	kinds of antimicrobial substance	1152:1183	Kirby-Bauer disk diffusion method was used to test the antibiotic resistance of A. viridians against kinds of antimicrobial substance.
28321788	6	55	theme	16S	1007:1009	arg1	RNA					1021:1023	16S ribosomal RNA	1007:1023	16S ribosomal RNA (rRNA) sequencing method	1007:1048	A. viridians isolates were further identified by API identification system and 16S ribosomal RNA (rRNA) sequencing method.
28321788	6	55	theme	16S	1007:1009	arg1	rRNA					1026:1029	rRNA	1026:1029	rRNA	1026:1029	A. viridians isolates were further identified by API identification system and 16S ribosomal RNA (rRNA) sequencing method.
28321788	3	56	theme	milk	616:619	arg1	yield					621:625	daily milk yield	610:625	daily milk yield	610:625	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	57	theme	individual	647:656	arg1	cow					658:660	individual cow	647:660	individual cow	647:660	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	0	58	theme	bovine	53:58	arg1	mastitis					72:79	bovine subclinical mastitis	53:79	bovine subclinical mastitis	53:79	Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
28321788	12	59	theme	infected	1779:1786	arg1	cows					1788:1791	the A. viridans infected cows	1763:1791	the A. viridans infected cows	1763:1791	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	10	60	theme	mastitis	1460:1467	arg1	highest					1499:1505	highest	1499:1505	highest	1499:1505	The incidence of mastitis caused by A. viridans was the highest (48-53%) during the summer season.
28321788	10	60	theme	mastitis	1460:1467	arg1	incidence					1447:1455	The incidence	1443:1455	The incidence of mastitis caused by A. viridans	1443:1489	The incidence of mastitis caused by A. viridans was the highest (48-53%) during the summer season.
28321788	0	61	from	effect	89:94	arg1	yield					109:113	yield	109:113	yield	109:113	Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
28321788	0	61	from	effect	89:94	arg1	composition					120:130	composition	120:130	composition	120:130	Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
28321788	0	61	from	effect	89:94	arg1	SCC					104:106	milk SCC	99:106	milk SCC	99:106	Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
28321788	12	62	theme	daily	1907:1911	arg1	yield					1918:1922	daily milk yield	1907:1922	daily milk yield	1907:1922	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	1	63	theme	Gram-positive	186:198	arg1	viridians					144:152	Aerococcus viridians	133:152	Aerococcus viridians (A. viridans)	133:166	Aerococcus viridians (A. viridans), an environmental Gram-positive bacterium, has been documented to be associated with bovine mastitis.
28321788	1	63	theme	Gram-positive	186:198	arg1	bacterium					200:208	an environmental Gram-positive bacterium	169:208	an environmental Gram-positive bacterium	169:208	Aerococcus viridians (A. viridans), an environmental Gram-positive bacterium, has been documented to be associated with bovine mastitis.
28321788	14	64	dep	agent	2276:2280	arg1	exerts					2324:2329	exerts	2324:2329	exerts an effect on SCC, milk yield, and composition	2324:2375	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	3	65	from	yield	621:625	arg1	SCC					604:606	SCC	604:606	SCC	604:606	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	65	from	yield	621:625	arg1	counts					596:601	the somatic cell counts	579:601	the somatic cell counts (SCC)	579:607	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	65	from	yield	621:625	arg1	udders					568:573	udders	568:573	udders	568:573	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	7	66	theme	antibiotic	1106:1115	arg1	resistance					1117:1126	the antibiotic resistance	1102:1126	the antibiotic resistance of A. viridians against kinds of antimicrobial substance	1102:1183	Kirby-Bauer disk diffusion method was used to test the antibiotic resistance of A. viridians against kinds of antimicrobial substance.
28321788	12	67	theme	A.	1767:1768	arg1	cows					1788:1791	the A. viridans infected cows	1763:1791	the A. viridans infected cows	1763:1791	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	8	68	theme	yield	1196:1200	arg1	data					1224:1227	SCC, milk yield, and milk composition data	1186:1227	SCC, milk yield, and milk composition data	1186:1227	SCC, milk yield, and milk composition data were from monthly Dairy Herd Improvement (DHI) results.
28321788	2	69	dep	changes	321:327	arg1	it					329:330	it	329:330	it	329:330	However, its exact role in bovine mastitis and the changes it brings about in milk characteristics are not yet known.
28321788	11	70	theme	antimicrobial	1595:1607	arg1	compounds					1609:1617	antimicrobial compounds	1595:1617	antimicrobial compounds tested	1595:1624	Majority of the isolates were susceptible to most of antimicrobial compounds tested, especially to β-lactams, but were found to be resistant (50-90%) to aminoglycosides, sulfonamides, and tetracycline.
28321788	7	71	theme	viridians	1134:1142	arg1	resistance					1117:1126	the antibiotic resistance	1102:1126	the antibiotic resistance of A. viridians against kinds of antimicrobial substance	1102:1183	Kirby-Bauer disk diffusion method was used to test the antibiotic resistance of A. viridians against kinds of antimicrobial substance.
28321788	3	72	theme	pathogen	556:563	arg1	yield					621:625	daily milk yield	610:625	daily milk yield	610:625	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	72	theme	pathogen	556:563	arg1	composition					632:642	composition	632:642	composition of individual cow	632:660	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	72	theme	pathogen	556:563	arg1	existence					538:546	existence	538:546	existence of this pathogen in udders and the somatic cell counts (SCC)	538:607	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	8	73	theme	SCC	1186:1188	arg1	data					1224:1227	SCC, milk yield, and milk composition data	1186:1227	SCC, milk yield, and milk composition data	1186:1227	SCC, milk yield, and milk composition data were from monthly Dairy Herd Improvement (DHI) results.
28321788	9	74	theme	bacterial	1382:1390	arg1	isolates					1392:1399	1674 bacterial isolates	1377:1399	1674 bacterial isolates cultured from milk samples with high SCC	1377:1440	Results showed that a total of 279 (16.67%) A. viridans isolates were identified from among 1674 bacterial isolates cultured from milk samples with high SCC.
28321788	6	75	theme	A.	928:929	arg1	isolates					941:948	A. viridians isolates	928:948	A. viridians isolates	928:948	A. viridians isolates were further identified by API identification system and 16S ribosomal RNA (rRNA) sequencing method.
28321788	8	76	theme	composition	1212:1222	arg1	data					1224:1227	SCC, milk yield, and milk composition data	1186:1227	SCC, milk yield, and milk composition data	1186:1227	SCC, milk yield, and milk composition data were from monthly Dairy Herd Improvement (DHI) results.
28321788	12	77	theme	healthy	1868:1874	arg1	72.4 × 103 cells/mL					1882:1900	72.4 × 103 cells/mL	1882:1900	72.4 × 103 cells/mL	1882:1900	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	12	77	theme	healthy	1868:1874	arg1	cows					1876:1879	healthy cows	1868:1879	healthy cows (72.4 × 103 cells/mL)	1868:1901	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	0	78	theme	milk	99:102	arg1	SCC					104:106	milk SCC	99:106	milk SCC	99:106	Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
28321788	11	79	dep	resistant	1673:1681	arg1	%					1689:1689	50-90%	1684:1689	50-90%	1684:1689	Majority of the isolates were susceptible to most of antimicrobial compounds tested, especially to β-lactams, but were found to be resistant (50-90%) to aminoglycosides, sulfonamides, and tetracycline.
28321788	6	80	dep	A.	928:929	arg1	viridians					931:939	viridians	931:939	viridians	931:939	A. viridians isolates were further identified by API identification system and 16S ribosomal RNA (rRNA) sequencing method.
28321788	8	81	theme	monthly	1239:1245	arg1	results					1276:1282	monthly Dairy Herd Improvement (DHI) results	1239:1282	monthly Dairy Herd Improvement (DHI) results	1239:1282	SCC, milk yield, and milk composition data were from monthly Dairy Herd Improvement (DHI) results.
28321788	4	82	from	2013	776:779	arg1	data					756:759	monthly DHI data	744:759	monthly DHI data from September 2013 to August 2014	744:794	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	9	83	theme	viridans	1332:1339	arg1	%					1326:1326	16.67%	1321:1326	16.67%	1321:1326	Results showed that a total of 279 (16.67%) A. viridans isolates were identified from among 1674 bacterial isolates cultured from milk samples with high SCC.
28321788	9	83	theme	viridans	1332:1339	arg1	isolates					1341:1348	279 (16.67%) A. viridans isolates	1316:1348	279 (16.67%) A. viridans isolates	1316:1348	Results showed that a total of 279 (16.67%) A. viridans isolates were identified from among 1674 bacterial isolates cultured from milk samples with high SCC.
28321788	8	84	theme	Herd	1253:1256	arg1	results					1276:1282	monthly Dairy Herd Improvement (DHI) results	1239:1282	monthly Dairy Herd Improvement (DHI) results	1239:1282	SCC, milk yield, and milk composition data were from monthly Dairy Herd Improvement (DHI) results.
28321788	2	85	theme	milk	348:351	arg1	characteristics					353:367	milk characteristics	348:367	milk characteristics	348:367	However, its exact role in bovine mastitis and the changes it brings about in milk characteristics are not yet known.
28321788	6	86	theme	identification	981:994	arg1	system					996:1001	API identification system	977:1001	API identification system	977:1001	A. viridians isolates were further identified by API identification system and 16S ribosomal RNA (rRNA) sequencing method.
28321788	0	87	theme	viridans	30:37	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics of Aerococcus viridans	0:37	Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
28321788	13	88	theme	milk	2129:2132	arg1	samples					2134:2140	milk samples	2129:2140	milk samples of cows infected with A. viridans	2129:2174	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	8	89	theme	Improvement	1258:1268	arg1	results					1276:1282	monthly Dairy Herd Improvement (DHI) results	1239:1282	monthly Dairy Herd Improvement (DHI) results	1239:1282	SCC, milk yield, and milk composition data were from monthly Dairy Herd Improvement (DHI) results.
28321788	3	90	theme	cell	591:594	arg1	SCC					604:606	SCC	604:606	SCC	604:606	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	3	90	theme	cell	591:594	arg1	counts					596:601	the somatic cell counts	579:601	the somatic cell counts (SCC)	579:607	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	4	91	theme	One-year	663:670	arg1	sampling					672:679	One-year sampling	663:679	One-year sampling for subclinical mastitis composite milk	663:719	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	7	92	theme	disk	1063:1066	arg1	method					1078:1083	Kirby-Bauer disk diffusion method	1051:1083	Kirby-Bauer disk diffusion method	1051:1083	Kirby-Bauer disk diffusion method was used to test the antibiotic resistance of A. viridians against kinds of antimicrobial substance.
28321788	9	93	theme	isolates	1341:1348	arg1	total					1307:1311	a total	1305:1311	a total of 279 (16.67%) A. viridans isolates	1305:1348	Results showed that a total of 279 (16.67%) A. viridans isolates were identified from among 1674 bacterial isolates cultured from milk samples with high SCC.
28321788	12	94	dep	decreased	1924:1932	arg1	P > 0.05					1935:1942	P > 0.05	1935:1942	P > 0.05	1935:1942	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	12	95	dep	higher	1811:1816	arg1	1000.0 × 103 cells/mL					1819:1839	1000.0 × 103 cells/mL	1819:1839	1000.0 × 103 cells/mL	1819:1839	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	12	95	dep	higher	1811:1816	arg1	P < 0.01					1843:1850	P < 0.01	1843:1850	P < 0.01	1843:1850	The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 103 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 103 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day.
28321788	14	96	theme	study	2197:2201	arg1	results					2181:2187	The results	2177:2187	The results of this study	2177:2201	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	3	97	theme	current	410:416	arg1	study					418:422	the current study	406:422	the current study	406:422	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	0	98	theme	subclinical	60:70	arg1	mastitis					72:79	bovine subclinical mastitis	53:79	bovine subclinical mastitis	53:79	Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
28321788	9	99	theme	milk	1415:1418	arg1	samples					1420:1426	milk samples	1415:1426	milk samples with high SCC	1415:1440	Results showed that a total of 279 (16.67%) A. viridans isolates were identified from among 1674 bacterial isolates cultured from milk samples with high SCC.
28321788	4	100	theme	DHI	752:754	arg1	data					756:759	monthly DHI data	744:759	monthly DHI data from September 2013 to August 2014	744:794	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	3	101	theme	antibiotic	445:454	arg1	resistance					456:465	the antibiotic resistance	441:465	the antibiotic resistance of A. viridans	441:480	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	14	102	theme	aetiological	2263:2274	arg1	viridans					2219:2226	A. viridans	2216:2226	A. viridans	2216:2226	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	14	102	theme	aetiological	2263:2274	arg1	agent					2276:2280	an emerging aetiological agent	2251:2280	an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition	2251:2375	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	4	103	theme	mastitis	697:704	arg1	milk					716:719	subclinical mastitis composite milk	685:719	subclinical mastitis composite milk	685:719	One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China.
28321788	10	104	dep	highest	1499:1505	arg1	%					1513:1513	48-53%	1508:1513	48-53%	1508:1513	The incidence of mastitis caused by A. viridans was the highest (48-53%) during the summer season.
28321788	6	105	theme	RNA	1021:1023	arg1	method					1043:1048	16S ribosomal RNA (rRNA) sequencing method	1007:1048	16S ribosomal RNA (rRNA) sequencing method	1007:1048	A. viridians isolates were further identified by API identification system and 16S ribosomal RNA (rRNA) sequencing method.
28321788	5	106	theme	microbiology	907:918	arg1	method					920:925	microbiology method	907:925	microbiology method	907:925	All samples were cultured and pathogens were identified using microbiology method.
28321788	7	107	theme	antimicrobial	1161:1173	arg1	substance					1175:1183	antimicrobial substance	1161:1183	antimicrobial substance	1161:1183	Kirby-Bauer disk diffusion method was used to test the antibiotic resistance of A. viridians against kinds of antimicrobial substance.
28321788	14	108	from	effect	2334:2339	arg1	SCC					2344:2346	SCC	2344:2346	SCC	2344:2346	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	14	108	from	effect	2334:2339	arg1	yield					2354:2358	milk yield	2349:2358	milk yield	2349:2358	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	14	108	from	effect	2334:2339	arg1	composition					2365:2375	composition	2365:2375	composition	2365:2375	The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
28321788	3	109	theme	viridans	473:480	arg1	resistance					456:465	the antibiotic resistance	441:465	the antibiotic resistance of A. viridans	441:480	The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow.
28321788	13	110	theme	fat	1994:1996	arg1	P > 0.05					2007:2014	P > 0.05	2007:2014	P > 0.05	2007:2014	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
28321788	13	110	theme	fat	1994:1996	arg1	content					1998:2004	fat content	1994:2004	fat content (P > 0.05)	1994:2015	Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans.
27956651	9	0	with	Samples	1519:1525	arg1	combination					1541:1551	a binary combination	1532:1551	a binary combination of BPP+C and BPP+I	1532:1570	Samples with a binary combination of BPP+C and BPP+I do not present a statistically significant difference in the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05).
27956651	2	1	theme	sausages	517:524	arg1	composition					405:415	chemical composition	396:415	chemical composition	396:415	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	1	theme	sausages	517:524	arg1	analysis					493:500	sensory analysis	485:500	sensory analysis	485:500	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	1	theme	sausages	517:524	arg1	stability					454:462	emulsion stability	445:462	emulsion stability	445:462	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	1	theme	sausages	517:524	arg1	loss					425:428	weight loss	418:428	weight loss after cooking	418:442	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	1	theme	sausages	517:524	arg1	properties					384:393	different properties	374:393	different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages)	374:525	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	1	theme	sausages	517:524	arg1	profile					473:479	texture profile	465:479	texture profile	465:479	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	6	2	theme	best	1145:1148	arg1	values					1160:1165	the best stability values	1141:1165	the best stability values	1141:1165	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	1	3	theme	plasma	231:236	arg1	BPP					248:250	BPP	248:250	BPP	248:250	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	1	3	theme	plasma	231:236	arg1	proteins					238:245	bovine plasma proteins	224:245	bovine plasma proteins (BPP)	224:251	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	9	4	theme	binary	1534:1539	arg1	combination					1541:1551	a binary combination	1532:1551	a binary combination of BPP+C and BPP+I	1532:1570	Samples with a binary combination of BPP+C and BPP+I do not present a statistically significant difference in the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05).
27956651	6	5	theme	5	1081:1081	arg1	%					1082:1082	%	1082:1082	%	1082:1082	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	2	6	theme	components	358:367	arg1	influence					339:347	The influence	335:347	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages)	335:525	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	1	7	theme	work	150:153	arg1	aim					138:140	The aim	134:140	The aim of this work	134:153	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	2	8	theme	agents	662:667	arg1	addition					640:647	addition	640:647	addition of texturing agents	640:667	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	4	9	contain	containing	927:936	arg2	components					953:962	the individual components	938:962	the individual components	938:962	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	4	9	contain	containing	927:936	arg1	samples					919:925	the samples	915:925	the samples containing the individual components	915:962	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	1	10	theme	overall	294:300	arg1	quality					302:308	the overall quality	290:308	the overall quality of fat-reduced sausages	290:332	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	11	11	theme	synergistic	1885:1895	arg1	effect					1897:1902	the synergistic effect	1881:1902	the synergistic effect of these binary combinations	1881:1931	However, when BPP combined with I or C, a major acceptability was obtained, demonstrating the synergistic effect of these binary combinations.
27956651	6	12	theme	BPP+C	1121:1125	arg1	combinations					1105:1116	binary combinations	1098:1116	binary combinations of BPP+C and BPP+I	1098:1135	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	6	12	theme	BPP+C	1121:1125	arg1	w/w					1089:1091	w/w	1089:1091	w/w	1089:1091	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	6	12	theme	BPP+C	1121:1125	arg1	BPP					1084:1086	5% BPP	1081:1086	5% BPP (w/w)	1081:1092	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	0	13	theme	plasma	117:122	arg1	proteins					124:131	bovine plasma proteins	110:131	bovine plasma proteins	110:131	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.
27956651	5	14	theme	content	986:992	arg1	reduction					969:977	The reduction	965:977	The reduction of fat content	965:992	The reduction of fat content increases the values of hardness and decreases the values of springiness.
27956651	1	15	from	influence	172:180	arg1	quality					302:308	the overall quality	290:308	the overall quality of fat-reduced sausages	290:332	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	2	16	theme	fat-reduced	613:623	arg1	sample					625:630	a fat-reduced sample	611:630	another a fat-reduced sample without addition of texturing agents	603:667	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	6	17	theme	fluid	1178:1182	arg1	loss					1184:1187	low total fluid loss	1168:1187	low total fluid loss	1168:1187	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	1	18	theme	sausages	325:332	arg1	quality					302:308	the overall quality	290:308	the overall quality of fat-reduced sausages	290:332	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	2	19	theme	sensory	485:491	arg1	properties					384:393	different properties	374:393	different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages)	374:525	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	19	theme	sensory	485:491	arg1	analysis					493:500	sensory analysis	485:500	sensory analysis	485:500	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	3	20	used	used	759:762	arg2	design					715:720	a full factorial experimental design	685:720	a full factorial experimental design of two levels with central point	685:753	In this sense, a full factorial experimental design of two levels with central point was used.
27956651	6	21	theme	low	1168:1170	arg1	loss					1184:1187	low total fluid loss	1168:1187	low total fluid loss	1168:1187	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	3	22	theme	factorial	692:700	arg1	design					715:720	a full factorial experimental design	685:720	a full factorial experimental design of two levels with central point	685:753	In this sense, a full factorial experimental design of two levels with central point was used.
27956651	3	23	with	design	715:720	arg1	point					749:753	central point	741:753	central point	741:753	In this sense, a full factorial experimental design of two levels with central point was used.
27956651	9	24	theme	BPP+I	1566:1570	arg1	combination					1541:1551	a binary combination	1532:1551	a binary combination of BPP+C and BPP+I	1532:1570	Samples with a binary combination of BPP+C and BPP+I do not present a statistically significant difference in the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05).
27956651	11	25	theme	combinations	1920:1931	arg1	effect					1897:1902	the synergistic effect	1881:1902	the synergistic effect of these binary combinations	1881:1931	However, when BPP combined with I or C, a major acceptability was obtained, demonstrating the synergistic effect of these binary combinations.
27956651	0	26	theme	combinations	69:80	arg1	Influence					37:45	Influence	37:45	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.	0:132	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.
27956651	1	27	theme	κ-carrageenan	269:281	arg1	combinations					208:219	the binary and ternary combinations	185:219	the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C)	185:285	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	2	28	theme	emulsion	445:452	arg1	properties					384:393	different properties	374:393	different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages)	374:525	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	28	theme	emulsion	445:452	arg1	stability					454:462	emulsion stability	445:462	emulsion stability	445:462	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	12	29	theme	fat-reduced	2059:2069	arg1	sausage					2071:2077	fat-reduced sausage	2059:2077	fat-reduced sausage	2059:2077	Therefore, our studies revealed that the binary combinations of BPP with I or C are good alternatives for the development of fat-reduced sausage.
27956651	0	30	theme	carrageenan	85:95	arg1	combinations					69:80	binary and ternary combinations	50:80	binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins	50:131	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.
27956651	7	31	theme	weight	1306:1311	arg1	loss					1313:1316	weight loss	1306:1316	weight loss	1306:1316	Similar results were obtained from the study of weight loss after cooking.
27956651	4	32	theme	higher	864:869	arg1	values					871:876	higher values	864:876	higher values of moisture and protein content	864:908	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	0	33	theme	inulin	98:103	arg1	combinations					69:80	binary and ternary combinations	50:80	binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins	50:131	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.
27956651	2	34	theme	chemical	396:403	arg1	properties					384:393	different properties	374:393	different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages)	374:525	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	34	theme	chemical	396:403	arg1	composition					405:415	chemical composition	396:415	chemical composition	396:415	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	9	35	theme	not-fat-reduced	1661:1675	arg1	sample					1688:1693	a not-fat-reduced commercial sample	1659:1693	a not-fat-reduced commercial sample ( P > 0.05)	1659:1705	Samples with a binary combination of BPP+C and BPP+I do not present a statistically significant difference in the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05).
27956651	9	35	theme	not-fat-reduced	1661:1675	arg1	P > 0.05					1697:1704	P > 0.05	1697:1704	P > 0.05	1697:1704	Samples with a binary combination of BPP+C and BPP+I do not present a statistically significant difference in the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05).
27956651	8	36	theme	BPP+I+C	1394:1400	arg1	destabilization					1364:1378	a destabilization	1362:1378	a destabilization of the sample BPP+I+C	1362:1400	However, both studies showed a destabilization of the sample BPP+I+C as emulsion stability decreased and weight loss increased after cooking compared to binary combinations ( P < 0.05).
27956651	1	37	theme	proteins	238:245	arg1	combinations					208:219	the binary and ternary combinations	185:219	the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C)	185:285	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	4	38	theme	individual	942:951	arg1	components					953:962	the individual components	938:962	the individual components	938:962	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	8	39	theme	emulsion	1405:1412	arg1	stability					1414:1422	emulsion stability	1405:1422	emulsion stability	1405:1422	However, both studies showed a destabilization of the sample BPP+I+C as emulsion stability decreased and weight loss increased after cooking compared to binary combinations ( P < 0.05).
27956651	0	40	theme	fat-reduced	15:25	arg1	sausages					27:34	fat-reduced sausages	15:34	fat-reduced sausages	15:34	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.
27956651	6	41	contain	had	1137:1139	arg2	values					1160:1165	the best stability values	1141:1165	the best stability values	1141:1165	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	6	41	contain	had	1137:1139	arg1	Samples					1068:1074	Samples	1068:1074	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I	1068:1135	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	6	41	contain	had	1137:1139	arg2	loss					1184:1187	low total fluid loss	1168:1187	low total fluid loss	1168:1187	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	4	42	theme	content	902:908	arg1	values					871:876	higher values	864:876	higher values of moisture and protein content	864:908	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	8	43	theme	weight	1438:1443	arg1	loss					1445:1448	weight loss	1438:1448	weight loss	1438:1448	However, both studies showed a destabilization of the sample BPP+I+C as emulsion stability decreased and weight loss increased after cooking compared to binary combinations ( P < 0.05).
27956651	12	44	theme	good	2018:2021	arg1	alternatives					2023:2034	good alternatives	2018:2034	good alternatives for the development of fat-reduced sausage	2018:2077	Therefore, our studies revealed that the binary combinations of BPP with I or C are good alternatives for the development of fat-reduced sausage.
27956651	12	44	theme	good	2018:2021	arg1	combinations					1982:1993	the binary combinations	1971:1993	the binary combinations of BPP with I or C	1971:2012	Therefore, our studies revealed that the binary combinations of BPP with I or C are good alternatives for the development of fat-reduced sausage.
27956651	12	45	theme	BPP	1998:2000	arg1	alternatives					2023:2034	good alternatives	2018:2034	good alternatives for the development of fat-reduced sausage	2018:2077	Therefore, our studies revealed that the binary combinations of BPP with I or C are good alternatives for the development of fat-reduced sausage.
27956651	12	45	theme	BPP	1998:2000	arg1	combinations					1982:1993	the binary combinations	1971:1993	the binary combinations of BPP with I or C	1971:2012	Therefore, our studies revealed that the binary combinations of BPP with I or C are good alternatives for the development of fat-reduced sausage.
27956651	11	46	theme	major	1833:1837	arg1	acceptability					1839:1851	a major acceptability	1831:1851	a major acceptability	1831:1851	However, when BPP combined with I or C, a major acceptability was obtained, demonstrating the synergistic effect of these binary combinations.
27956651	9	47	from	difference	1615:1624	arg1	chewiness					1633:1641	the chewiness	1629:1641	the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05)	1629:1705	Samples with a binary combination of BPP+C and BPP+I do not present a statistically significant difference in the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05).
27956651	4	48	contain	containing	777:786	arg2	BPP+I					788:792	BPP+I	788:792	BPP+I	788:792	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	4	48	contain	containing	777:786	arg1	samples					769:775	The samples	765:775	The samples containing BPP+I and BPP+C	765:802	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	4	48	contain	containing	777:786	arg2	BPP+C					798:802	BPP+C	798:802	BPP+C	798:802	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	0	49	dep	Development	0:10	arg1	Influence					37:45	Influence	37:45	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.	0:132	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.
27956651	4	50	attach	presented	854:862	arg1	synergy					813:819	a synergy	811:819	a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components	811:962	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	4	50	attach	presented	854:862	arg2	combinations					841:852	the binary combinations	830:852	the binary combinations	830:852	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	5	51	theme	springiness	1055:1065	arg1	values					1045:1050	the values	1041:1050	the values of springiness	1041:1065	The reduction of fat content increases the values of hardness and decreases the values of springiness.
27956651	1	52	theme	combinations	208:219	arg1	influence					172:180	the influence	168:180	the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages	168:332	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	6	53	theme	stability	1150:1158	arg1	values					1160:1165	the best stability values	1141:1165	the best stability values	1141:1165	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	11	54	theme	binary	1913:1918	arg1	combinations					1920:1931	these binary combinations	1907:1931	these binary combinations	1907:1931	However, when BPP combined with I or C, a major acceptability was obtained, demonstrating the synergistic effect of these binary combinations.
27956651	1	55	theme	bovine	224:229	arg1	BPP					248:250	BPP	248:250	BPP	248:250	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	1	55	theme	bovine	224:229	arg1	proteins					238:245	bovine plasma proteins	224:245	bovine plasma proteins (BPP)	224:251	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	2	56	theme	fat-reduced	505:515	arg1	sausages					517:524	fat-reduced sausages	505:524	fat-reduced sausages	505:524	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	6	57	theme	%	1082:1082	arg1	w/w					1089:1091	w/w	1089:1091	w/w	1089:1091	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	6	57	theme	%	1082:1082	arg1	BPP					1084:1086	5% BPP	1081:1086	5% BPP (w/w)	1081:1092	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	7	58	theme	loss	1313:1316	arg1	study					1297:1301	the study	1293:1301	the study of weight loss	1293:1316	Similar results were obtained from the study of weight loss after cooking.
27956651	10	59	contain	containing	1770:1779	arg1	sample					1728:1733	The less acceptable sample	1708:1733	The less acceptable sample for flavor and texture	1708:1756	The less acceptable sample for flavor and texture was the one containing only BPP.
27956651	10	59	contain	containing	1770:1779	arg1	one					1766:1768	one	1766:1768	one	1766:1768	The less acceptable sample for flavor and texture was the one containing only BPP.
27956651	10	59	contain	containing	1770:1779	arg2	BPP					1786:1788	only BPP	1781:1788	only BPP	1781:1788	The less acceptable sample for flavor and texture was the one containing only BPP.
27956651	6	60	theme	BPP+I	1131:1135	arg1	combinations					1105:1116	binary combinations	1098:1116	binary combinations of BPP+C and BPP+I	1098:1135	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	6	60	theme	BPP+I	1131:1135	arg1	w/w					1089:1091	w/w	1089:1091	w/w	1089:1091	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	6	60	theme	BPP+I	1131:1135	arg1	BPP					1084:1086	5% BPP	1081:1086	5% BPP (w/w)	1081:1092	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	9	61	theme	BPP+C	1556:1560	arg1	combination					1541:1551	a binary combination	1532:1551	a binary combination of BPP+C and BPP+I	1532:1570	Samples with a binary combination of BPP+C and BPP+I do not present a statistically significant difference in the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05).
27956651	12	62	theme	binary	1975:1980	arg1	alternatives					2023:2034	good alternatives	2018:2034	good alternatives for the development of fat-reduced sausage	2018:2077	Therefore, our studies revealed that the binary combinations of BPP with I or C are good alternatives for the development of fat-reduced sausage.
27956651	12	62	theme	binary	1975:1980	arg1	combinations					1982:1993	the binary combinations	1971:1993	the binary combinations of BPP with I or C	1971:2012	Therefore, our studies revealed that the binary combinations of BPP with I or C are good alternatives for the development of fat-reduced sausage.
27956651	0	63	theme	bovine	110:115	arg1	proteins					124:131	bovine plasma proteins	110:131	bovine plasma proteins	110:131	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.
27956651	2	64	theme	texture	465:471	arg1	properties					384:393	different properties	374:393	different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages)	374:525	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	64	theme	texture	465:471	arg1	profile					473:479	texture profile	465:479	texture profile	465:479	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	0	65	theme	proteins	124:131	arg1	combinations					69:80	binary and ternary combinations	50:80	binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins	50:131	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.
27956651	5	66	theme	fat	982:984	arg1	content					986:992	fat content	982:992	fat content	982:992	The reduction of fat content increases the values of hardness and decreases the values of springiness.
27956651	7	67	theme	Similar	1258:1264	arg1	results					1266:1272	Similar results	1258:1272	Similar results	1258:1272	Similar results were obtained from the study of weight loss after cooking.
27956651	3	68	theme	full	687:690	arg1	design					715:720	a full factorial experimental design	685:720	a full factorial experimental design of two levels with central point	685:753	In this sense, a full factorial experimental design of two levels with central point was used.
27956651	1	69	theme	fat-reduced	313:323	arg1	sausages					325:332	fat-reduced sausages	313:332	fat-reduced sausages	313:332	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	6	70	with	Samples	1068:1074	arg1	combinations					1105:1116	binary combinations	1098:1116	binary combinations of BPP+C and BPP+I	1098:1135	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	6	70	with	Samples	1068:1074	arg1	w/w					1089:1091	w/w	1089:1091	w/w	1089:1091	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	6	70	with	Samples	1068:1074	arg1	BPP					1084:1086	5% BPP	1081:1086	5% BPP (w/w)	1081:1092	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	6	71	theme	binary	1098:1103	arg1	combinations					1105:1116	binary combinations	1098:1116	binary combinations of BPP+C and BPP+I	1098:1135	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	6	72	theme	total	1172:1176	arg1	loss					1184:1187	low total fluid loss	1168:1187	low total fluid loss	1168:1187	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	9	73	theme	significant	1603:1613	arg1	difference					1615:1624	a statistically significant difference	1587:1624	a statistically significant difference in the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05)	1587:1705	Samples with a binary combination of BPP+C and BPP+I do not present a statistically significant difference in the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05).
27956651	2	74	theme	texturing	652:660	arg1	agents					662:667	texturing agents	652:667	texturing agents	652:667	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	0	75	theme	ternary	61:67	arg1	combinations					69:80	binary and ternary combinations	50:80	binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins	50:131	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.
27956651	2	76	theme	weight	418:423	arg1	properties					384:393	different properties	374:393	different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages)	374:525	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	76	theme	weight	418:423	arg1	loss					425:428	weight loss	418:428	weight loss after cooking	418:442	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	3	77	theme	experimental	702:713	arg1	design					715:720	a full factorial experimental design	685:720	a full factorial experimental design of two levels with central point	685:753	In this sense, a full factorial experimental design of two levels with central point was used.
27956651	2	78	dep	properties	384:393	arg1	composition					405:415	chemical composition	396:415	chemical composition	396:415	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	78	dep	properties	384:393	arg1	analysis					493:500	sensory analysis	485:500	sensory analysis	485:500	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	78	dep	properties	384:393	arg1	stability					454:462	emulsion stability	445:462	emulsion stability	445:462	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	78	dep	properties	384:393	arg1	loss					425:428	weight loss	418:428	weight loss after cooking	418:442	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	78	dep	properties	384:393	arg1	properties					384:393	different properties	374:393	different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages)	374:525	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	78	dep	properties	384:393	arg1	profile					473:479	texture profile	465:479	texture profile	465:479	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	4	79	theme	moisture	881:888	arg1	content					902:908	moisture and protein content	881:908	content	902:908	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	3	80	theme	central	741:747	arg1	point					749:753	central point	741:753	central point	741:753	In this sense, a full factorial experimental design of two levels with central point was used.
27956651	4	81	theme	binary	834:839	arg1	combinations					841:852	the binary combinations	830:852	the binary combinations	830:852	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	8	82	theme	sample	1387:1392	arg1	BPP+I+C					1394:1400	the sample BPP+I+C	1383:1400	the sample BPP+I+C	1383:1400	However, both studies showed a destabilization of the sample BPP+I+C as emulsion stability decreased and weight loss increased after cooking compared to binary combinations ( P < 0.05).
27956651	3	83	theme	levels	729:734	arg1	design					715:720	a full factorial experimental design	685:720	a full factorial experimental design of two levels with central point	685:753	In this sense, a full factorial experimental design of two levels with central point was used.
27956651	6	84	theme	synergistic	1219:1229	arg1	effect					1231:1236	a significant synergistic effect	1205:1236	a significant synergistic effect	1205:1236	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	12	85	theme	sausage	2071:2077	arg1	development					2044:2054	the development	2040:2054	the development of fat-reduced sausage	2040:2077	Therefore, our studies revealed that the binary combinations of BPP with I or C are good alternatives for the development of fat-reduced sausage.
27956651	5	86	theme	hardness	1018:1025	arg1	values					1008:1013	the values	1004:1013	the values of hardness	1004:1025	The reduction of fat content increases the values of hardness and decreases the values of springiness.
27956651	9	87	theme	commercial	1677:1686	arg1	sample					1688:1693	a not-fat-reduced commercial sample	1659:1693	a not-fat-reduced commercial sample ( P > 0.05)	1659:1705	Samples with a binary combination of BPP+C and BPP+I do not present a statistically significant difference in the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05).
27956651	9	87	theme	commercial	1677:1686	arg1	P > 0.05					1697:1704	P > 0.05	1697:1704	P > 0.05	1697:1704	Samples with a binary combination of BPP+C and BPP+I do not present a statistically significant difference in the chewiness with respect to a not-fat-reduced commercial sample ( P > 0.05).
27956651	2	88	theme	fat	585:587	arg1	reduction					589:597	fat reduction	585:597	fat reduction	585:597	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	0	89	theme	sausages	27:34	arg1	Development					0:10	Development	0:10	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.	0:132	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.
27956651	4	90	theme	protein	894:900	arg1	content					902:908	moisture and protein content	881:908	content	902:908	The samples containing BPP+I and BPP+C showed a synergy in which the binary combinations presented higher values of moisture and protein content than the samples containing the individual components.
27956651	10	91	theme	acceptable	1717:1726	arg1	sample					1728:1733	The less acceptable sample	1708:1733	The less acceptable sample for flavor and texture	1708:1756	The less acceptable sample for flavor and texture was the one containing only BPP.
27956651	10	91	theme	acceptable	1717:1726	arg1	one					1766:1768	one	1766:1768	one	1766:1768	The less acceptable sample for flavor and texture was the one containing only BPP.
27956651	12	92	with	combinations	1982:1993	arg1	C					2012:2012	C	2012:2012	C	2012:2012	Therefore, our studies revealed that the binary combinations of BPP with I or C are good alternatives for the development of fat-reduced sausage.
27956651	12	92	with	combinations	1982:1993	arg1	I					2007:2007	I	2007:2007	I	2007:2007	Therefore, our studies revealed that the binary combinations of BPP with I or C are good alternatives for the development of fat-reduced sausage.
27956651	6	93	theme	significant	1207:1217	arg1	effect					1231:1236	a significant synergistic effect	1205:1236	a significant synergistic effect	1205:1236	Samples with 5% BPP (w/w) and binary combinations of BPP+C and BPP+I had the best stability values (low total fluid loss), demonstrating a significant synergistic effect by combining BPP+C.
27956651	0	94	theme	binary	50:55	arg1	combinations					69:80	binary and ternary combinations	50:80	binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins	50:131	Development of fat-reduced sausages: Influence of binary and ternary combinations of carrageenan, inulin, and bovine plasma proteins.
27956651	1	95	theme	inulin	254:259	arg1	combinations					208:219	the binary and ternary combinations	185:219	the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C)	185:285	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	2	96	theme	different	374:382	arg1	composition					405:415	chemical composition	396:415	chemical composition	396:415	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	96	theme	different	374:382	arg1	analysis					493:500	sensory analysis	485:500	sensory analysis	485:500	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	96	theme	different	374:382	arg1	stability					454:462	emulsion stability	445:462	emulsion stability	445:462	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	96	theme	different	374:382	arg1	loss					425:428	weight loss	418:428	weight loss after cooking	418:442	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	96	theme	different	374:382	arg1	properties					384:393	different properties	374:393	different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages)	374:525	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	2	96	theme	different	374:382	arg1	profile					473:479	texture profile	465:479	texture profile	465:479	The influence of these components over different properties (chemical composition, weight loss after cooking, emulsion stability, texture profile and sensory analysis of fat-reduced sausages) was studied and compared against two samples, one without fat reduction and another a fat-reduced sample without addition of texturing agents.
27956651	1	97	theme	binary	189:194	arg1	combinations					208:219	the binary and ternary combinations	185:219	the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C)	185:285	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
27956651	8	98	theme	binary	1486:1491	arg1	P < 0.05					1508:1515	P < 0.05	1508:1515	P < 0.05	1508:1515	However, both studies showed a destabilization of the sample BPP+I+C as emulsion stability decreased and weight loss increased after cooking compared to binary combinations ( P < 0.05).
27956651	8	98	theme	binary	1486:1491	arg1	combinations					1493:1504	binary combinations	1486:1504	binary combinations ( P < 0.05)	1486:1516	However, both studies showed a destabilization of the sample BPP+I+C as emulsion stability decreased and weight loss increased after cooking compared to binary combinations ( P < 0.05).
27956651	10	99	theme	only	1781:1784	arg1	BPP					1786:1788	only BPP	1781:1788	only BPP	1781:1788	The less acceptable sample for flavor and texture was the one containing only BPP.
27956651	1	100	theme	ternary	200:206	arg1	combinations					208:219	the binary and ternary combinations	185:219	the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C)	185:285	The aim of this work was to study the influence of the binary and ternary combinations of bovine plasma proteins (BPP), inulin (I) and κ-carrageenan (C) in the overall quality of fat-reduced sausages.
29275198	2	0	theme	GT	392:393	arg1	weight					378:383	weight	378:383	weight of dry GT used in the solutions	378:415	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	4	1	theme	proper	873:878	arg1	strength					888:895	proper tensile strength	873:895	proper tensile strength	873:895	Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite.
29275198	1	2	theme	degradable	235:244	arg1	GT					262:263	GT	262:263	GT	262:263	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
29275198	1	2	theme	degradable	235:244	arg1	tragacanth					250:259	bio degradable gum tragacanth	231:259	bio degradable gum tragacanth (GT)	231:264	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
29275198	0	3	theme	gum	74:76	arg1	tragacanth					78:87	gum tragacanth	74:87	gum tragacanth	74:87	Production of cotton fabrics with durable antibacterial property by using gum tragacanth and silver.
29275198	3	4	theme	antibacterial	737:749	arg1	activity					751:758	the antibacterial activity	733:758	the antibacterial activity of fabrics	733:769	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	2	5	dep	according	365:373	arg1	%					362:362	5%	361:362	5%	361:362	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	4	6	with	fabric	931:936	arg1	composite					952:960	GT-6%/Ag composite	943:960	GT-6%/Ag composite	943:960	Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite.
29275198	4	7	theme	/Ag	948:950	arg1	composite					952:960	GT-6%/Ag composite	943:960	GT-6%/Ag composite	943:960	Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite.
29275198	1	8	theme	gum	246:248	arg1	GT					262:263	GT	262:263	GT	262:263	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
29275198	1	8	theme	gum	246:248	arg1	tragacanth					250:259	bio degradable gum tragacanth	231:259	bio degradable gum tragacanth (GT)	231:264	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
29275198	2	9	theme	physical	555:562	arg1	characteristics					591:605	physical, mechanical and biological characteristics	555:605	physical, mechanical and biological characteristics of cotton fabric	555:622	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	4	10	theme	%	947:947	arg1	composite					952:960	GT-6%/Ag composite	943:960	GT-6%/Ag composite	943:960	Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite.
29275198	4	11	theme	treated	834:840	arg1	cotton					842:847	the treated cotton	830:847	the treated cotton with GT-4%/Ag	830:861	Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite.
29275198	3	12	theme	only	811:814	arg1	GT					816:817	only GT	811:817	only GT	811:817	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	4	13	theme	GT-4	854:857	arg1	%					858:858	%	858:858	%	858:858	Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite.
29275198	3	14	from	presence	649:656	arg1	structure					700:708	the composite structure	686:708	the composite structure	686:708	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	5	15	theme	GT	991:992	arg1	biocompatibility					967:982	The biocompatibility	963:982	The biocompatibility of the GT and GT/Ag treated fabrics	963:1018	The biocompatibility of the GT and GT/Ag treated fabrics was verified through MTT assay on fibroblast cells.
29275198	1	16	theme	antibacterial	164:176	arg1	fabric					185:190	antibacterial cotton fabric	164:190	antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT)	164:264	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
29275198	2	17	theme	dry	388:390	arg1	GT					392:393	dry GT	388:393	dry GT used in the solutions	388:415	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	18	theme	biological	580:589	arg1	characteristics					591:605	physical, mechanical and biological characteristics	555:605	physical, mechanical and biological characteristics of cotton fabric	555:622	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	19	dep	GT	305:306	arg1	4					312:312	4	312:312	4	312:312	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	19	dep	GT	305:306	arg1	2					309:309	2	309:309	2	309:309	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	19	dep	GT	305:306	arg1	6 g/L					318:322	6 g/L	318:322	6 g/L	318:322	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	1	20	theme	cotton	178:183	arg1	fabric					185:190	antibacterial cotton fabric	164:190	antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT)	164:264	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
29275198	5	21	theme	fabrics	1012:1018	arg1	biocompatibility					967:982	The biocompatibility	963:982	The biocompatibility of the GT and GT/Ag treated fabrics	963:1018	The biocompatibility of the GT and GT/Ag treated fabrics was verified through MTT assay on fibroblast cells.
29275198	3	22	theme	small	661:665	arg1	AgNPs					677:681	AgNPs	677:681	AgNPs	677:681	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	3	22	theme	small	661:665	arg1	amount					667:672	small amount	661:672	small amount of AgNPs	661:681	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	2	23	theme	different	277:285	arg1	concentrations					287:300	different concentrations	277:300	different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions)	277:416	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	5	24	from	assay	1045:1049	arg1	cells					1065:1069	fibroblast cells	1054:1069	fibroblast cells	1054:1069	The biocompatibility of the GT and GT/Ag treated fabrics was verified through MTT assay on fibroblast cells.
29275198	0	25	theme	fabrics	21:27	arg1	Production					0:9	Production	0:9	Production of cotton fabrics with durable antibacterial property	0:63	Production of cotton fabrics with durable antibacterial property by using gum tragacanth and silver.
29275198	3	26	theme	amount	667:672	arg1	presence					649:656	the presence	645:656	the presence of small amount of AgNPs in the composite structure	645:708	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	2	27	theme	mechanical	565:574	arg1	characteristics					591:605	physical, mechanical and biological characteristics	555:605	physical, mechanical and biological characteristics of cotton fabric	555:622	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	28	theme	Ag	357:358	arg1	amount					347:352	a constant amount	336:352	GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions)	305:416	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	28	theme	Ag	357:358	arg1	Ag					357:358	Ag	357:358	Ag (5%; according to weight of dry GT used in the solutions)	357:416	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	0	29	theme	cotton	14:19	arg1	fabrics					21:27	cotton fabrics	14:27	cotton fabrics with durable antibacterial property	14:63	Production of cotton fabrics with durable antibacterial property by using gum tragacanth and silver.
29275198	5	30	theme	treated	1004:1010	arg1	fabrics					1012:1018	the GT and GT/Ag treated fabrics	987:1018	fabrics	1012:1018	The biocompatibility of the GT and GT/Ag treated fabrics was verified through MTT assay on fibroblast cells.
29275198	1	31	theme	silver	198:203	arg1	AgNPs					220:224	AgNPs	220:224	AgNPs	220:224	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
29275198	1	31	theme	silver	198:203	arg1	nanoparticles					205:217	silver nanoparticles	198:217	silver nanoparticles (AgNPs)	198:225	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
29275198	0	32	theme	durable	34:40	arg1	property					56:63	durable antibacterial property	34:63	durable antibacterial property	34:63	Production of cotton fabrics with durable antibacterial property by using gum tragacanth and silver.
29275198	2	33	theme	amount	347:352	arg1	concentrations					287:300	different concentrations	277:300	different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions)	277:416	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	4	34	theme	tensile	880:886	arg1	strength					888:895	proper tensile strength	873:895	proper tensile strength	873:895	Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite.
29275198	2	35	from	efficacy	450:457	arg1	characteristics					591:605	physical, mechanical and biological characteristics	555:605	physical, mechanical and biological characteristics of cotton fabric	555:622	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	3	36	dep	was	710:712	arg1	exhibited					635:643	exhibited	635:643	exhibited	635:643	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	0	37	with	fabrics	21:27	arg1	property					56:63	durable antibacterial property	34:63	durable antibacterial property	34:63	Production of cotton fabrics with durable antibacterial property by using gum tragacanth and silver.
29275198	2	38	theme	constant	338:345	arg1	amount					347:352	a constant amount	336:352	GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions)	305:416	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	38	theme	constant	338:345	arg1	Ag					357:358	Ag	357:358	Ag (5%; according to weight of dry GT used in the solutions)	357:416	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	39	theme	potency	476:482	arg1	effect					545:550	their effect	539:550	their effect on physical, mechanical and biological characteristics of cotton fabric	539:622	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	39	theme	potency	476:482	arg1	efficacy					450:457	the efficacy	446:457	the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus	446:533	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	5	40	theme	fibroblast	1054:1063	arg1	cells					1065:1069	fibroblast cells	1054:1069	fibroblast cells	1054:1069	The biocompatibility of the GT and GT/Ag treated fabrics was verified through MTT assay on fibroblast cells.
29275198	2	41	dep	Ag	357:358	arg1	according					365:373	according	365:373	according to weight of dry GT used in the solutions	365:415	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	42	theme	fabric	617:622	arg1	characteristics					591:605	physical, mechanical and biological characteristics	555:605	physical, mechanical and biological characteristics of cotton fabric	555:622	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	1	43	theme	simple	103:108	arg1	procedure					126:134	A simple and inexpensive procedure	101:134	A simple and inexpensive procedure	101:134	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
29275198	0	44	theme	antibacterial	42:54	arg1	property					56:63	durable antibacterial property	34:63	durable antibacterial property	34:63	Production of cotton fabrics with durable antibacterial property by using gum tragacanth and silver.
29275198	5	45	theme	MTT	1041:1043	arg1	assay					1045:1049	MTT assay	1041:1049	MTT assay on fibroblast cells	1041:1069	The biocompatibility of the GT and GT/Ag treated fabrics was verified through MTT assay on fibroblast cells.
29275198	2	46	theme	antibacterial	462:474	arg1	potency					476:482	antibacterial potency	462:482	antibacterial potency against Escherichia coli and Staphylococcus aureus	462:533	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	47	theme	cotton	610:615	arg1	fabric					617:622	cotton fabric	610:622	cotton fabric	610:622	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	2	48	from	effect	545:550	arg1	characteristics					591:605	physical, mechanical and biological characteristics	555:605	physical, mechanical and biological characteristics of cotton fabric	555:622	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	3	49	theme	fabrics	763:769	arg1	activity					751:758	the antibacterial activity	733:758	the antibacterial activity of fabrics	733:769	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	3	50	theme	AgNPs	677:681	arg1	AgNPs					677:681	AgNPs	677:681	AgNPs	677:681	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	3	50	theme	AgNPs	677:681	arg1	amount					667:672	small amount	661:672	small amount of AgNPs	661:681	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	2	51	dep	along	325:329	arg1	with					331:334	with	331:334	with	331:334	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	1	52	theme	inexpensive	114:124	arg1	procedure					126:134	A simple and inexpensive procedure	101:134	A simple and inexpensive procedure	101:134	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
29275198	3	53	attach	presence	649:656	arg2	AgNPs					677:681	AgNPs	677:681	AgNPs	677:681	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	3	53	attach	presence	649:656	arg2	amount					667:672	small amount	661:672	small amount of AgNPs	661:681	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	3	53	attach	presence	649:656	arg1	structure					700:708	the composite structure	686:708	the composite structure	686:708	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	2	54	theme	GT	305:306	arg1	concentrations					287:300	different concentrations	277:300	different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions)	277:416	For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric.
29275198	3	55	theme	composite	690:698	arg1	structure					700:708	the composite structure	686:708	the composite structure	686:708	Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT.
29275198	4	56	theme	%	858:858	arg1	/Ag					859:861	GT-4%/Ag	854:861	GT-4%/Ag	854:861	Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite.
29275198	5	57	theme	GT/Ag	998:1002	arg1	fabrics					1012:1018	the GT and GT/Ag treated fabrics	987:1018	fabrics	1012:1018	The biocompatibility of the GT and GT/Ag treated fabrics was verified through MTT assay on fibroblast cells.
29275198	4	58	theme	treated	923:929	arg1	fabric					931:936	treated fabric	923:936	treated fabric with GT-6%/Ag composite	923:960	Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite.
29275198	4	59	with	cotton	842:847	arg1	/Ag					859:861	GT-4%/Ag	854:861	GT-4%/Ag	854:861	Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite.
29275198	4	60	theme	GT-6	943:946	arg1	%					947:947	%	947:947	%	947:947	Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite.
29275198	1	61	theme	bio	231:233	arg1	GT					262:263	GT	262:263	GT	262:263	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
29275198	1	61	theme	bio	231:233	arg1	tragacanth					250:259	bio degradable gum tragacanth	231:259	bio degradable gum tragacanth (GT)	231:264	A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT).
26938880	8	0	theme	interactive	1201:1211	arg1	exploration					1213:1223	simultaneous interactive exploration	1188:1223	simultaneous interactive exploration of all time points	1188:1242	The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points.
26938880	10	1	theme	important	1610:1618	arg1	lipids					1656:1661	lipids	1656:1661	lipids	1656:1661	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	10	1	theme	important	1610:1618	arg1	compounds					1620:1628	agronomically and industrially important compounds	1579:1628	agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents	1579:1683	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	10	1	theme	important	1610:1618	arg1	oligosaccharides					1638:1653	oligosaccharides	1638:1653	oligosaccharides	1638:1653	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	10	1	theme	important	1610:1618	arg1	agents					1678:1683	antifungal agents	1667:1683	antifungal agents	1667:1683	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	5	2	theme	tissue-specific	686:700	arg1	localizations					702:714	tissue-specific localizations	686:714	tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates	686:778	26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates.
26938880	11	3	theme	on-tissue	1753:1761	arg1	mapping					1782:1788	on-tissue metabolite profile mapping	1753:1788	on-tissue metabolite profile mapping	1753:1788	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
26938880	6	4	theme	MSI	868:870	arg1	sets					877:880	multiple MSI data sets	859:880	multiple MSI data sets from a time series	859:899	In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch.
26938880	8	5	theme	time	1232:1235	arg1	points					1237:1242	all time points	1228:1242	all time points	1228:1242	The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points.
26938880	4	6	from	dependency	487:496	arg1	compositions					518:529	their fatty acid compositions	501:529	their fatty acid compositions	501:529	Lipids in the endosperm co-localized in dependency on their fatty acid compositions with changes in the distributions of diacyl phosphatidylcholines during germination.
26938880	9	7	theme	new	1256:1258	arg1	approach					1269:1276	this new analysis approach	1251:1276	this new analysis approach	1251:1276	Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered.
26938880	6	8	theme	metabolite	834:843	arg1	compositions					845:856	local metabolite compositions	828:856	local metabolite compositions	828:856	In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch.
26938880	10	9	theme	antifungal	1667:1676	arg1	agents					1678:1683	antifungal agents	1667:1683	antifungal agents	1667:1683	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	11	10	theme	high	1883:1886	arg1	interest					1899:1906	high scientific interest	1883:1906	high scientific interest	1883:1906	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
26938880	9	11	theme	visualization	1282:1294	arg1	tool					1296:1299	visualization tool	1282:1299	visualization tool	1282:1299	Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered.
26938880	1	12	theme	barley	197:202	arg1	germination					204:214	the barley germination	193:214	the barley germination	193:214	MALDI mass spectrometry imaging was performed to localize metabolites during the first seven days of the barley germination.
26938880	5	13	theme	derivates	770:778	arg1	localizations					702:714	tissue-specific localizations	686:714	tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates	686:778	26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates.
26938880	9	14	theme	germination-dependent	1301:1321	arg1	developments					1323:1334	germination-dependent developments	1301:1334	germination-dependent developments of metabolite patterns with single MS position accuracy	1301:1390	Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered.
26938880	5	15	theme	glycosylated	725:736	arg1	derivates					770:778	their glycosylated, hydroxylated, and O-methylated derivates	719:778	their glycosylated, hydroxylated, and O-methylated derivates	719:778	26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates.
26938880	11	16	theme	germination	1850:1860	arg1	process					1862:1868	the germination process	1846:1868	the germination process	1846:1868	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
26938880	5	17	theme	hydroxylated	739:750	arg1	derivates					770:778	their glycosylated, hydroxylated, and O-methylated derivates	719:778	their glycosylated, hydroxylated, and O-methylated derivates	719:778	26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates.
26938880	9	18	theme	patterns	1350:1357	arg1	developments					1323:1334	germination-dependent developments	1301:1334	germination-dependent developments of metabolite patterns with single MS position accuracy	1301:1390	Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered.
26938880	7	19	theme	new	1028:1030	arg1	strategy					1032:1039	a new strategy	1026:1039	comparability between data sets as well as a new strategy for unsupervised clustering	983:1067	This requires a new preprocessing strategy to achieve comparability between data sets as well as a new strategy for unsupervised clustering.
26938880	5	20	gly	glycosylated	725:736	arg1	derivates					770:778	their glycosylated, hydroxylated, and O-methylated derivates	719:778	their glycosylated, hydroxylated, and O-methylated derivates	719:778	26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates.
26938880	9	21	theme	single	1364:1369	arg1	accuracy					1383:1390	single MS position accuracy	1364:1390	single MS position accuracy	1364:1390	Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered.
26938880	9	22	theme	position	1374:1381	arg1	accuracy					1383:1390	single MS position accuracy	1364:1390	single MS position accuracy	1364:1390	Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered.
26938880	4	23	theme	acid	513:516	arg1	compositions					518:529	their fatty acid compositions	501:529	their fatty acid compositions	501:529	Lipids in the endosperm co-localized in dependency on their fatty acid compositions with changes in the distributions of diacyl phosphatidylcholines during germination.
26938880	10	24	theme	MALDI	1515:1519	arg1	MSI					1521:1523	MALDI MSI	1515:1523	MALDI MSI	1515:1523	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	8	25	theme	spatial	1084:1090	arg1	segmentation					1092:1103	The resulting spatial segmentation	1070:1103	The resulting spatial segmentation for each time point sample	1070:1130	The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points.
26938880	7	26	theme	data	1005:1008	arg1	sets					1010:1013	data sets	1005:1013	data sets	1005:1013	This requires a new preprocessing strategy to achieve comparability between data sets as well as a new strategy for unsupervised clustering.
26938880	3	27	theme	embryo	439:444	arg1	parts					416:420	parts	416:420	parts of the developed embryo	416:444	Oligosaccharides were observed in the endosperm and in parts of the developed embryo.
26938880	0	28	theme	Spatio-Temporal	0:14	arg1	Profiling					27:35	Spatio-Temporal Metabolite Profiling	0:35	Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS	0:81	Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging.
26938880	8	29	theme	time	1114:1117	arg1	sample					1125:1130	each time point sample	1109:1130	each time point sample	1109:1130	The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points.
26938880	1	30	theme	MALDI	92:96	arg1	spectrometry					103:114	MALDI mass spectrometry	92:114	MALDI mass spectrometry imaging	92:122	MALDI mass spectrometry imaging was performed to localize metabolites during the first seven days of the barley germination.
26938880	10	31	theme	peaks	1570:1574	arg1	number					1560:1565	a large number	1552:1565	a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents	1552:1683	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	9	32	with	developments	1323:1334	arg1	accuracy					1383:1390	single MS position accuracy	1364:1390	single MS position accuracy	1364:1390	Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered.
26938880	1	33	theme	spectrometry	103:114	arg1	imaging					116:122	MALDI mass spectrometry imaging	92:122	MALDI mass spectrometry imaging	92:122	MALDI mass spectrometry imaging was performed to localize metabolites during the first seven days of the barley germination.
26938880	4	34	theme	diacyl	568:573	arg1	phosphatidylcholines					575:594	diacyl phosphatidylcholines	568:594	diacyl phosphatidylcholines	568:594	Lipids in the endosperm co-localized in dependency on their fatty acid compositions with changes in the distributions of diacyl phosphatidylcholines during germination.
26938880	10	35	theme	number	1560:1565	arg1	identification					1534:1547	the identification	1530:1547	the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents	1530:1683	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	11	36	theme	important	1799:1807	arg1	information					1809:1819	important information	1799:1819	important information for the understanding of the germination process, which is of high scientific interest	1799:1906	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
26938880	10	37	theme	first	1421:1425	arg1	study					1427:1431	the first study	1417:1431	the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents	1417:1683	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	10	37	theme	first	1421:1425	arg1	This					1409:1412	This	1409:1412	This	1409:1412	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	2	38	theme	tissue-specific	330:344	arg1	localizations					346:358	highly tissue-specific localizations	323:358	highly tissue-specific localizations	323:358	Up to 100 mass signals were detected of which 85 signals were identified as 48 different metabolites with highly tissue-specific localizations.
26938880	0	39	theme	Germination	51:61	arg1	Process					63:69	the Barley Germination Process	40:69	the Barley Germination Process	40:69	Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging.
26938880	9	40	dep	discovered	1397:1406	arg1	Using					1245:1249	Using	1245:1249	Using this new analysis approach and visualization tool	1245:1299	Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered.
26938880	8	41	theme	cluster	1164:1170	arg1	map					1172:1174	an interactive cluster map	1149:1174	an interactive cluster map	1149:1174	The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points.
26938880	6	42	from	patterns	816:823	arg1	compositions					845:856	local metabolite compositions	828:856	local metabolite compositions	828:856	In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch.
26938880	10	43	theme	metabolite	1447:1456	arg1	profiling					1458:1466	metabolite profiling	1447:1466	metabolite profiling of a cereals' germination process over time	1447:1510	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	7	44	theme	new	945:947	arg1	strategy					963:970	a new preprocessing strategy	943:970	a new preprocessing strategy to achieve comparability between data sets as well as a new strategy for unsupervised clustering	943:1067	This requires a new preprocessing strategy to achieve comparability between data sets as well as a new strategy for unsupervised clustering.
26938880	4	45	from	changes	536:542	arg1	distributions					551:563	the distributions	547:563	the distributions of diacyl phosphatidylcholines during germination	547:613	Lipids in the endosperm co-localized in dependency on their fatty acid compositions with changes in the distributions of diacyl phosphatidylcholines during germination.
26938880	10	46	theme	compounds	1620:1628	arg1	peaks					1570:1574	peaks	1570:1574	peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents	1570:1683	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	11	47	theme	cluster	1732:1738	arg1	analyses					1740:1747	the MS cluster analyses	1725:1747	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping	1686:1788	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
26938880	5	48	located	detected	658:665	arg1	embryo					674:679	the embryo	670:679	the embryo	670:679	26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates.
26938880	5	48	located	detected	658:665	arg2	hordatines					642:651	26 potentially antifungal hordatines	616:651	26 potentially antifungal hordatines	616:651	26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates.
26938880	8	49	theme	simultaneous	1188:1199	arg1	exploration					1213:1223	simultaneous interactive exploration	1188:1223	simultaneous interactive exploration of all time points	1188:1242	The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points.
26938880	6	50	theme	data	872:875	arg1	sets					877:880	multiple MSI data sets	859:880	multiple MSI data sets from a time series	859:899	In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch.
26938880	11	51	theme	metabolite	1763:1772	arg1	mapping					1782:1788	on-tissue metabolite profile mapping	1753:1788	on-tissue metabolite profile mapping	1753:1788	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
26938880	6	52	theme	multiple	859:866	arg1	sets					877:880	multiple MSI data sets	859:880	multiple MSI data sets from a time series	859:899	In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch.
26938880	1	53	theme	first	173:177	arg1	days					185:188	the first seven days	169:188	the first seven days of the barley germination	169:214	MALDI mass spectrometry imaging was performed to localize metabolites during the first seven days of the barley germination.
26938880	9	54	theme	analysis	1260:1267	arg1	approach					1269:1276	this new analysis approach	1251:1276	this new analysis approach	1251:1276	Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered.
26938880	2	55	theme	different	296:304	arg1	signals					266:272	85 signals	263:272	85 signals	263:272	Up to 100 mass signals were detected of which 85 signals were identified as 48 different metabolites with highly tissue-specific localizations.
26938880	2	55	theme	different	296:304	arg1	metabolites					306:316	48 different metabolites	293:316	48 different metabolites with highly tissue-specific localizations	293:358	Up to 100 mass signals were detected of which 85 signals were identified as 48 different metabolites with highly tissue-specific localizations.
26938880	8	56	theme	points	1237:1242	arg1	exploration					1213:1223	simultaneous interactive exploration	1188:1223	simultaneous interactive exploration of all time points	1188:1242	The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points.
26938880	6	57	theme	local	828:832	arg1	compositions					845:856	local metabolite compositions	828:856	local metabolite compositions	828:856	In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch.
26938880	11	58	theme	scientific	1888:1897	arg1	interest					1899:1906	high scientific interest	1883:1906	high scientific interest	1883:1906	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
26938880	5	59	theme	O-methylated	757:768	arg1	derivates					770:778	their glycosylated, hydroxylated, and O-methylated derivates	719:778	their glycosylated, hydroxylated, and O-methylated derivates	719:778	26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates.
26938880	2	60	theme	mass	227:230	arg1	signals					232:238	Up to 100 mass signals	217:238	Up to 100 mass signals	217:238	Up to 100 mass signals were detected of which 85 signals were identified as 48 different metabolites with highly tissue-specific localizations.
26938880	1	61	theme	germination	204:214	arg1	days					185:188	the first seven days	169:188	the first seven days of the barley germination	169:214	MALDI mass spectrometry imaging was performed to localize metabolites during the first seven days of the barley germination.
26938880	2	62	dep	100	223:225	arg1	to					220:221	to	220:221	to	220:221	Up to 100 mass signals were detected of which 85 signals were identified as 48 different metabolites with highly tissue-specific localizations.
26938880	6	63	from	series	894:899	arg1	sets					877:880	multiple MSI data sets	859:880	multiple MSI data sets from a time series	859:899	In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch.
26938880	10	64	theme	germination	1482:1492	arg1	process					1494:1500	a cereals' germination process	1471:1500	a cereals' germination process over time	1471:1510	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	9	65	theme	metabolite	1339:1348	arg1	patterns					1350:1357	metabolite patterns	1339:1357	metabolite patterns	1339:1357	Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered.
26938880	7	66	theme	unsupervised	1045:1056	arg1	clustering					1058:1067	unsupervised clustering	1045:1067	unsupervised clustering	1045:1067	This requires a new preprocessing strategy to achieve comparability between data sets as well as a new strategy for unsupervised clustering.
26938880	11	67	theme	process	1862:1868	arg1	understanding					1829:1841	the understanding	1825:1841	the understanding of the germination process, which is of high scientific interest	1825:1906	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
26938880	0	68	theme	MALDI	74:78	arg1	MS					80:81	MALDI MS	74:81	MALDI MS	74:81	Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging.
26938880	4	69	theme	fatty	507:511	arg1	compositions					518:529	their fatty acid compositions	501:529	their fatty acid compositions	501:529	Lipids in the endosperm co-localized in dependency on their fatty acid compositions with changes in the distributions of diacyl phosphatidylcholines during germination.
26938880	9	70	theme	MS	1371:1372	arg1	accuracy					1383:1390	single MS position accuracy	1364:1390	single MS position accuracy	1364:1390	Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered.
26938880	8	71	theme	resulting	1074:1082	arg1	segmentation					1092:1103	The resulting spatial segmentation	1070:1103	The resulting spatial segmentation for each time point sample	1070:1130	The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points.
26938880	5	72	theme	antifungal	631:640	arg1	hordatines					642:651	26 potentially antifungal hordatines	616:651	26 potentially antifungal hordatines	616:651	26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates.
26938880	0	73	theme	Metabolite	16:25	arg1	Profiling					27:35	Spatio-Temporal Metabolite Profiling	0:35	Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS	0:81	Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging.
26938880	8	74	theme	point	1119:1123	arg1	sample					1125:1130	each time point sample	1109:1130	each time point sample	1109:1130	The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points.
26938880	3	75	theme	developed	429:437	arg1	embryo					439:444	the developed embryo	425:444	the developed embryo	425:444	Oligosaccharides were observed in the endosperm and in parts of the developed embryo.
26938880	1	76	theme	mass	98:101	arg1	spectrometry					103:114	MALDI mass spectrometry	92:114	MALDI mass spectrometry imaging	92:122	MALDI mass spectrometry imaging was performed to localize metabolites during the first seven days of the barley germination.
26938880	4	77	theme	phosphatidylcholines	575:594	arg1	distributions					551:563	the distributions	547:563	the distributions of diacyl phosphatidylcholines during germination	547:613	Lipids in the endosperm co-localized in dependency on their fatty acid compositions with changes in the distributions of diacyl phosphatidylcholines during germination.
26938880	0	78	theme	Barley	44:49	arg1	Process					63:69	the Barley Germination Process	40:69	the Barley Germination Process	40:69	Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging.
26938880	2	79	with	metabolites	306:316	arg1	localizations					346:358	highly tissue-specific localizations	323:358	highly tissue-specific localizations	323:358	Up to 100 mass signals were detected of which 85 signals were identified as 48 different metabolites with highly tissue-specific localizations.
26938880	10	80	theme	large	1554:1558	arg1	number					1560:1565	a large number	1552:1565	a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents	1552:1683	This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents.
26938880	11	81	theme	profile	1774:1780	arg1	mapping					1782:1788	on-tissue metabolite profile mapping	1753:1788	on-tissue metabolite profile mapping	1753:1788	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
26938880	0	82	theme	Process	63:69	arg1	Profiling					27:35	Spatio-Temporal Metabolite Profiling	0:35	Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS	0:81	Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging.
26938880	8	83	theme	interactive	1152:1162	arg1	map					1172:1174	an interactive cluster map	1149:1174	an interactive cluster map	1149:1174	The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points.
26938880	4	84	from	Lipids	447:452	arg1	endosperm					461:469	the endosperm	457:469	the endosperm	457:469	Lipids in the endosperm co-localized in dependency on their fatty acid compositions with changes in the distributions of diacyl phosphatidylcholines during germination.
26938880	6	85	theme	spatio-temporal	800:814	arg1	patterns					816:823	spatio-temporal patterns	800:823	spatio-temporal patterns in local metabolite compositions	800:856	In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch.
26938880	7	86	theme	preprocessing	949:961	arg1	strategy					963:970	a new preprocessing strategy	943:970	a new preprocessing strategy to achieve comparability between data sets as well as a new strategy for unsupervised clustering	943:1067	This requires a new preprocessing strategy to achieve comparability between data sets as well as a new strategy for unsupervised clustering.
26938880	6	87	theme	time	889:892	arg1	series					894:899	a time series	887:899	a time series	887:899	In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch.
26938880	3	88	located	observed	383:390	arg1	endosperm					399:407	the endosperm	395:407	the endosperm	395:407	Oligosaccharides were observed in the endosperm and in parts of the developed embryo.
26938880	3	88	located	observed	383:390	arg2	Oligosaccharides					361:376	Oligosaccharides	361:376	Oligosaccharides	361:376	Oligosaccharides were observed in the endosperm and in parts of the developed embryo.
26938880	11	89	theme	MS	1729:1730	arg1	analyses					1740:1747	the MS cluster analyses	1725:1747	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping	1686:1788	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
26938880	11	90	theme	detailed	1692:1699	arg1	localization					1701:1712	Their detailed localization	1686:1712	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping	1686:1788	Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
25666613	0	0	theme	proteins	84:91	arg1	CpsABCD					93:99	the proteins CpsABCD	80:99	the proteins CpsABCD	80:99	Streptococcus agalactiae capsule polymer length and attachment is determined by the proteins CpsABCD.
25666613	1	1	theme	prototypical	243:254	arg1	mechanism					256:264	a prototypical mechanism	241:264	a prototypical mechanism to produce these structures	241:292	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	1	2	theme	secreted	154:161	arg1	exopolysaccharides					163:180	secreted exopolysaccharides	154:180	secreted exopolysaccharides	154:180	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	9	3	theme	molecular	1378:1386	arg1	weight					1388:1393	high molecular weight	1373:1393	high molecular weight polysaccharides	1373:1409	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	7	4	theme	phosphatase	1158:1168	arg1	target					1131:1136	the target	1127:1136	the target of the cognate CpsB phosphatase	1127:1168	These Tyr residues are also the target of the cognate CpsB phosphatase.
25666613	7	4	theme	phosphatase	1158:1168	arg1	residues					1109:1116	These Tyr residues	1099:1116	These Tyr residues	1099:1116	These Tyr residues are also the target of the cognate CpsB phosphatase.
25666613	4	5	theme	knock-out	770:778	arg1	strains					802:808	knock-out and functional mutant strains	770:808	knock-out and functional mutant strains	770:808	We used this human pathogen to investigate the roles and potential interdependencies of the conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutant strains.
25666613	9	6	attach	attachment	1440:1449	arg1	surface					1484:1490	the bacterial cell surface	1465:1490	the bacterial cell surface	1465:1490	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	9	6	attach	attachment	1440:1449	arg2	CPS					1458:1460	the CPS	1454:1460	the CPS	1454:1460	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	9	7	theme	weight	1388:1393	arg1	polysaccharides					1395:1409	high molecular weight polysaccharides	1373:1409	high molecular weight polysaccharides	1373:1409	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	10	8	theme	cell	1746:1749	arg1	wall					1751:1754	the cell wall	1742:1754	the cell wall	1742:1754	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	8	9	theme	CpsC	1296:1299	arg1	action					1286:1291	the subsequent action	1271:1291	the subsequent action of CpsC	1271:1299	An interaction between CpsD and CpsC was observed, and the phosphorylation state of CpsD influenced the subsequent action of CpsC.
25666613	6	10	theme	CpsC-dependent	1076:1089	arg1	manner					1091:1096	a CpsC-dependent manner	1074:1096	a CpsC-dependent manner	1074:1096	We observed that CpsB, -C, and -D compose a phosphoregulatory system where the CpsD autokinase phosphorylates its C-terminal tyrosines in a CpsC-dependent manner.
25666613	7	11	theme	cognate	1145:1151	arg1	phosphatase					1158:1168	the cognate CpsB phosphatase	1141:1168	the cognate CpsB phosphatase	1141:1168	These Tyr residues are also the target of the cognate CpsB phosphatase.
25666613	10	12	theme	CPS	1666:1668	arg1	biosynthesis					1670:1681	CPS biosynthesis	1666:1681	CPS biosynthesis	1666:1681	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	3	13	theme	virulence	522:530	arg1	factor					532:537	the main virulence factor	513:537	the main virulence factor of the bacterium	513:554	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	9	14	theme	CpsA-mediated	1426:1438	arg1	attachment					1440:1449	CpsA-mediated attachment	1426:1449	CpsA-mediated attachment of the CPS to the bacterial cell surface	1426:1490	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	1	15	theme	exopolysaccharides	163:180	arg1	ubiquitous					185:194	ubiquitous	185:194	ubiquitous	185:194	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	1	15	theme	exopolysaccharides	163:180	arg1	production					106:115	The production	102:115	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides	102:180	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	7	16	theme	CpsB	1153:1156	arg1	phosphatase					1158:1168	the cognate CpsB phosphatase	1141:1168	the cognate CpsB phosphatase	1141:1168	These Tyr residues are also the target of the cognate CpsB phosphatase.
25666613	9	17	theme	high	1373:1376	arg1	weight					1388:1393	high molecular weight	1373:1393	high molecular weight polysaccharides	1373:1409	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	9	18	theme	bacterial	1469:1477	arg1	surface					1484:1490	the bacterial cell surface	1465:1490	the bacterial cell surface	1465:1490	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	3	19	theme	group	454:458	arg1	Streptococcus					462:474	group B Streptococcus	454:474	group B Streptococcus	454:474	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	3	19	theme	group	454:458	arg1	agalactiae					442:451	Streptococcus agalactiae	428:451	Streptococcus agalactiae (group B Streptococcus; GBS)	428:480	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	9	20	theme	CpsC	1306:1309	arg1	domain					1325:1330	The CpsC extracellular domain	1302:1330	The CpsC extracellular domain	1302:1330	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	2	21	theme	polysaccharide	322:335	arg1	composition					337:347	polysaccharide composition	322:347	polysaccharide composition among species	322:361	Despite the differences in polysaccharide composition among species, a group of proteins involved in this pathway is well conserved.
25666613	3	22	theme	Streptococcus	428:440	arg1	Streptococcus					462:474	group B Streptococcus	454:474	group B Streptococcus	454:474	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	3	22	theme	Streptococcus	428:440	arg1	agalactiae					442:451	Streptococcus agalactiae	428:451	Streptococcus agalactiae (group B Streptococcus; GBS)	428:480	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	0	23	theme	Streptococcus	0:12	arg1	length					41:46	Streptococcus agalactiae capsule polymer length	0:46	Streptococcus agalactiae capsule polymer length	0:46	Streptococcus agalactiae capsule polymer length and attachment is determined by the proteins CpsABCD.
25666613	10	24	theme	basal	1579:1583	arg1	unit					1595:1598	the basal repeating unit	1575:1598	the basal repeating unit	1575:1598	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	10	25	theme	last	1652:1655	arg1	steps					1657:1661	the last steps	1648:1661	the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall)	1648:1755	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	6	26	theme	CpsD	1015:1018	arg1	autokinase					1020:1029	the CpsD autokinase	1011:1029	the CpsD autokinase	1011:1029	We observed that CpsB, -C, and -D compose a phosphoregulatory system where the CpsD autokinase phosphorylates its C-terminal tyrosines in a CpsC-dependent manner.
25666613	0	27	theme	polymer	33:39	arg1	length					41:46	Streptococcus agalactiae capsule polymer length	0:46	Streptococcus agalactiae capsule polymer length	0:46	Streptococcus agalactiae capsule polymer length and attachment is determined by the proteins CpsABCD.
25666613	4	28	theme	mutant	795:800	arg1	strains					802:808	knock-out and functional mutant strains	770:808	knock-out and functional mutant strains	770:808	We used this human pathogen to investigate the roles and potential interdependencies of the conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutant strains.
25666613	8	29	theme	CpsD	1255:1258	arg1	state					1246:1250	the phosphorylation state	1226:1250	the phosphorylation state of CpsD	1226:1258	An interaction between CpsD and CpsC was observed, and the phosphorylation state of CpsD influenced the subsequent action of CpsC.
25666613	3	30	theme	bacterium	546:554	arg1	factor					532:537	the main virulence factor	513:537	the main virulence factor of the bacterium	513:554	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	3	31	from	target	571:576	arg1	development					597:607	current vaccine development	581:607	current vaccine development	581:607	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	0	32	theme	capsule	25:31	arg1	length					41:46	Streptococcus agalactiae capsule polymer length	0:46	Streptococcus agalactiae capsule polymer length	0:46	Streptococcus agalactiae capsule polymer length and attachment is determined by the proteins CpsABCD.
25666613	9	33	theme	CPS	1458:1460	arg1	attachment					1440:1449	CpsA-mediated attachment	1426:1449	CpsA-mediated attachment of the CPS to the bacterial cell surface	1426:1490	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	9	34	theme	cell	1479:1482	arg1	surface					1484:1490	the bacterial cell surface	1465:1490	the bacterial cell surface	1465:1490	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	6	35	theme	C-terminal	1050:1059	arg1	tyrosines					1061:1069	its C-terminal tyrosines	1046:1069	its C-terminal tyrosines	1046:1069	We observed that CpsB, -C, and -D compose a phosphoregulatory system where the CpsD autokinase phosphorylates its C-terminal tyrosines in a CpsC-dependent manner.
25666613	9	36	theme	polysaccharides	1395:1409	arg1	production					1359:1368	the production	1355:1368	the production of high molecular weight polysaccharides	1355:1409	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	4	37	theme	potential	667:675	arg1	interdependencies					677:693	potential interdependencies	667:693	potential interdependencies	667:693	We used this human pathogen to investigate the roles and potential interdependencies of the conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutant strains.
25666613	10	38	theme	cps	1537:1539	arg1	transcription					1541:1553	cps transcription	1537:1553	cps transcription	1537:1553	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	5	39	theme	cell	890:893	arg1	surface					895:901	the cell surface	886:901	the cell surface	886:901	The mutant strains were examined for CPS quantity, size, and attachment to the cell surface as well as CpsD phosphorylation.
25666613	8	40	theme	phosphorylation	1230:1244	arg1	state					1246:1250	the phosphorylation state	1226:1250	the phosphorylation state of CpsD	1226:1258	An interaction between CpsD and CpsC was observed, and the phosphorylation state of CpsD influenced the subsequent action of CpsC.
25666613	8	41	theme	subsequent	1275:1284	arg1	action					1286:1291	the subsequent action	1271:1291	the subsequent action of CpsC	1271:1299	An interaction between CpsD and CpsC was observed, and the phosphorylation state of CpsD influenced the subsequent action of CpsC.
25666613	3	42	theme	B	460:460	arg1	Streptococcus					462:474	group B Streptococcus	454:474	group B Streptococcus	454:474	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	3	42	theme	B	460:460	arg1	agalactiae					442:451	Streptococcus agalactiae	428:451	Streptococcus agalactiae (group B Streptococcus; GBS)	428:480	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	5	43	theme	CPS	848:850	arg1	quantity					852:859	CPS quantity	848:859	CPS quantity	848:859	The mutant strains were examined for CPS quantity, size, and attachment to the cell surface as well as CpsD phosphorylation.
25666613	9	44	theme	extracellular	1311:1323	arg1	domain					1325:1330	The CpsC extracellular domain	1302:1330	The CpsC extracellular domain	1302:1330	The CpsC extracellular domain appeared necessary for the production of high molecular weight polysaccharides by influencing CpsA-mediated attachment of the CPS to the bacterial cell surface.
25666613	10	45	theme	unit	1595:1598	arg1	transcription					1541:1553	cps transcription	1537:1553	cps transcription	1537:1553	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	10	45	theme	unit	1595:1598	arg1	synthesis					1562:1570	the synthesis	1558:1570	the synthesis of the basal repeating unit	1558:1598	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	10	46	from	impact	1527:1532	arg1	transcription					1541:1553	cps transcription	1537:1553	cps transcription	1537:1553	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	10	46	from	impact	1527:1532	arg1	synthesis					1562:1570	the synthesis	1558:1570	the synthesis of the basal repeating unit	1558:1598	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	3	47	theme	current	581:587	arg1	development					597:607	current vaccine development	581:607	current vaccine development	581:607	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	4	48	theme	human	623:627	arg1	pathogen					629:636	this human pathogen	618:636	this human pathogen	618:636	We used this human pathogen to investigate the roles and potential interdependencies of the conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutant strains.
25666613	7	49	theme	Tyr	1105:1107	arg1	target					1131:1136	the target	1127:1136	the target of the cognate CpsB phosphatase	1127:1168	These Tyr residues are also the target of the cognate CpsB phosphatase.
25666613	7	49	theme	Tyr	1105:1107	arg1	residues					1109:1116	These Tyr residues	1099:1116	These Tyr residues	1099:1116	These Tyr residues are also the target of the cognate CpsB phosphatase.
25666613	5	50	theme	mutant	815:820	arg1	strains					822:828	The mutant strains	811:828	The mutant strains	811:828	The mutant strains were examined for CPS quantity, size, and attachment to the cell surface as well as CpsD phosphorylation.
25666613	3	51	theme	prime	565:569	arg1	target					571:576	a prime target	563:576	a prime target in current vaccine development	563:607	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	4	52	theme	conserved	702:710	arg1	CpsABCD					721:727	the conserved proteins CpsABCD	698:727	the conserved proteins CpsABCD encoded in the cps operon	698:753	We used this human pathogen to investigate the roles and potential interdependencies of the conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutant strains.
25666613	5	53	theme	CpsD	914:917	arg1	phosphorylation					919:933	CpsD phosphorylation	914:933	CpsD phosphorylation	914:933	The mutant strains were examined for CPS quantity, size, and attachment to the cell surface as well as CpsD phosphorylation.
25666613	10	54	dep	balance	1693:1699	arg1	i.e.					1684:1687	i.e.	1684:1687	i.e.	1684:1687	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	1	55	from	bacteria	199:206	arg1	ubiquitous					185:194	ubiquitous	185:194	ubiquitous	185:194	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	1	55	from	bacteria	199:206	arg1	production					106:115	The production	102:115	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides	102:180	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	1	56	theme	capsular	120:127	arg1	polysaccharides					129:143	capsular polysaccharides	120:143	capsular polysaccharides (CPS)	120:149	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	1	56	theme	capsular	120:127	arg1	CPS					146:148	CPS	146:148	CPS	146:148	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	4	57	theme	functional	784:793	arg1	strains					802:808	knock-out and functional mutant strains	770:808	knock-out and functional mutant strains	770:808	We used this human pathogen to investigate the roles and potential interdependencies of the conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutant strains.
25666613	1	58	theme	polysaccharides	129:143	arg1	ubiquitous					185:194	ubiquitous	185:194	ubiquitous	185:194	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	1	58	theme	polysaccharides	129:143	arg1	production					106:115	The production	102:115	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides	102:180	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	4	59	theme	cps	744:746	arg1	operon					748:753	the cps operon	740:753	the cps operon	740:753	We used this human pathogen to investigate the roles and potential interdependencies of the conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutant strains.
25666613	1	60	theme	Wzy	217:219	arg1	pathway					221:227	the Wzy pathway	213:227	the Wzy pathway	213:227	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	3	61	dep	Streptococcus	462:474	arg1	GBS					477:479	GBS	477:479	GBS	477:479	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	1	62	from	ubiquitous	185:194	arg1	bacteria					199:206	bacteria	199:206	bacteria	199:206	The production of capsular polysaccharides (CPS) or secreted exopolysaccharides is ubiquitous in bacteria, and the Wzy pathway constitutes a prototypical mechanism to produce these structures.
25666613	4	63	theme	proteins	712:719	arg1	CpsABCD					721:727	the conserved proteins CpsABCD	698:727	the conserved proteins CpsABCD encoded in the cps operon	698:753	We used this human pathogen to investigate the roles and potential interdependencies of the conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutant strains.
25666613	4	64	theme	CpsABCD	721:727	arg1	interdependencies					677:693	potential interdependencies	667:693	potential interdependencies	667:693	We used this human pathogen to investigate the roles and potential interdependencies of the conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutant strains.
25666613	4	64	theme	CpsABCD	721:727	arg1	roles					657:661	the roles	653:661	the roles	653:661	We used this human pathogen to investigate the roles and potential interdependencies of the conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutant strains.
25666613	6	65	theme	phosphoregulatory	980:996	arg1	system					998:1003	a phosphoregulatory system	978:1003	a phosphoregulatory system where the CpsD autokinase phosphorylates its C-terminal tyrosines in a CpsC-dependent manner	978:1096	We observed that CpsB, -C, and -D compose a phosphoregulatory system where the CpsD autokinase phosphorylates its C-terminal tyrosines in a CpsC-dependent manner.
25666613	3	66	theme	vaccine	589:595	arg1	development					597:607	current vaccine development	581:607	current vaccine development	581:607	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	2	67	theme	proteins	375:382	arg1	group					366:370	a group	364:370	a group of proteins involved in this pathway	364:407	Despite the differences in polysaccharide composition among species, a group of proteins involved in this pathway is well conserved.
25666613	2	67	theme	proteins	375:382	arg1	proteins					375:382	proteins	375:382	proteins involved in this pathway	375:407	Despite the differences in polysaccharide composition among species, a group of proteins involved in this pathway is well conserved.
25666613	10	68	theme	repeating	1585:1593	arg1	unit					1595:1598	the basal repeating unit	1575:1598	the basal repeating unit	1575:1598	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	10	69	theme	biosynthesis	1670:1681	arg1	steps					1657:1661	the last steps	1648:1661	the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall)	1648:1755	In conclusion, although having no impact on cps transcription or the synthesis of the basal repeating unit, we suggest that these proteins are fine-tuning the last steps of CPS biosynthesis (i.e. the balance between polymerization and attachment to the cell wall).
25666613	0	70	dep	Streptococcus	0:12	arg1	agalactiae					14:23	agalactiae	14:23	agalactiae	14:23	Streptococcus agalactiae capsule polymer length and attachment is determined by the proteins CpsABCD.
25666613	4	71	used	used	613:616	arg2	We					610:611	We	610:611	We	610:611	We used this human pathogen to investigate the roles and potential interdependencies of the conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutant strains.
25666613	3	72	theme	main	517:520	arg1	factor					532:537	the main virulence factor	513:537	the main virulence factor of the bacterium	513:554	Streptococcus agalactiae (group B Streptococcus; GBS) produces a CPS that represents the main virulence factor of the bacterium and is a prime target in current vaccine development.
25666613	2	73	from	differences	307:317	arg1	composition					337:347	polysaccharide composition	322:347	polysaccharide composition among species	322:361	Despite the differences in polysaccharide composition among species, a group of proteins involved in this pathway is well conserved.
25434126	0	0	theme	chiral	108:113	arg1	phase					126:130	cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase	64:130	cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase	64:130	[Chromatographic separation of aminoglutethimide enantiomers on cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase].
25434126	5	1	theme	different	727:735	arg1	n-hexane/ethanol					759:774	n-hexane/ethanol	759:774	n-hexane/ethanol	759:774	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	5	1	theme	different	727:735	arg1	compositions					745:756	two different solvent compositions	723:756	two different solvent compositions	723:756	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	5	1	theme	different	727:735	arg1	n-hexane/isopropanol					780:799	n-hexane/isopropanol	780:799	n-hexane/isopropanol	780:799	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	11	2	theme	continuous	1551:1560	arg1	production					1562:1571	the batch and continuous production	1537:1571	the batch and continuous production of aminoglutethimide enantiomers	1537:1604	This work provides the basic information of chromatographic separation for the batch and continuous production of aminoglutethimide enantiomers.
25434126	2	3	theme	side	342:345	arg1	effects					347:353	side effects	342:353	side effects	342:353	It was reported that S-(-)-AG enantiomer had small activity and sometimes might cause side effects.
25434126	7	4	with	n-hexane-ethanol	991:1006	arg1	monoethanolamine					1031:1046	0.1% monoethanolamine additive	1026:1055	0.1% monoethanolamine additive	1026:1055	According to the experiments, n-hexane-ethanol (30:70, v/v) with 0.1% monoethanolamine additive was selected as the mobile phase.
25434126	5	5	theme	solvent	737:743	arg1	n-hexane/ethanol					759:774	n-hexane/ethanol	759:774	n-hexane/ethanol	759:774	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	5	5	theme	solvent	737:743	arg1	compositions					745:756	two different solvent compositions	723:756	two different solvent compositions	723:756	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	5	5	theme	solvent	737:743	arg1	n-hexane/isopropanol					780:799	n-hexane/isopropanol	780:799	n-hexane/isopropanol	780:799	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	0	6	from	separation	17:26	arg1	phase					126:130	cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase	64:130	cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase	64:130	[Chromatographic separation of aminoglutethimide enantiomers on cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase].
25434126	8	7	theme	0	1295:1295	arg1	flow-rate					1282:1290	a flow-rate	1280:1290	a flow-rate of 0	1280:1295	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	9	8	theme	stationary	1366:1375	arg1	30:70					1405:1409	30:70	1405:1409	30:70	1405:1409	6 mL/min and column temperature of 25-40 °C, with Chiralcel OD-H as stationary phase and n-hexane-ethanol (30:70, v/v) with 0.
25434126	9	8	theme	stationary	1366:1375	arg1	phase					1377:1381	stationary phase	1366:1381	stationary phase	1366:1381	6 mL/min and column temperature of 25-40 °C, with Chiralcel OD-H as stationary phase and n-hexane-ethanol (30:70, v/v) with 0.
25434126	11	9	theme	enantiomers	1594:1604	arg1	production					1562:1571	the batch and continuous production	1537:1571	the batch and continuous production of aminoglutethimide enantiomers	1537:1604	This work provides the basic information of chromatographic separation for the batch and continuous production of aminoglutethimide enantiomers.
25434126	9	10	with	°C	1339:1340	arg1	Chiralcel					1348:1356	Chiralcel	1348:1356	Chiralcel	1348:1356	6 mL/min and column temperature of 25-40 °C, with Chiralcel OD-H as stationary phase and n-hexane-ethanol (30:70, v/v) with 0.
25434126	0	11	theme	stationary	115:124	arg1	phase					126:130	cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase	64:130	cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase	64:130	[Chromatographic separation of aminoglutethimide enantiomers on cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase].
25434126	11	12	theme	batch	1541:1545	arg1	production					1562:1571	the batch and continuous production	1537:1571	the batch and continuous production of aminoglutethimide enantiomers	1537:1604	This work provides the basic information of chromatographic separation for the batch and continuous production of aminoglutethimide enantiomers.
25434126	6	13	theme	AG	931:932	arg1	performance					908:918	the separation performance	893:918	the separation performance of racemic AG by HPLC	893:940	The effects of alcohol content and monoethanolamine additive on the separation performance of racemic AG by HPLC were investigated.
25434126	1	14	used	used	166:169	arg2	Aminoglutethimide					134:150	Aminoglutethimide	134:150	Aminoglutethimide (AG)	134:155	Aminoglutethimide (AG) has been used clinically as a drug in the treatment of hormone-dependent metastatic breast cancer.
25434126	1	14	used	used	166:169	arg2	drug					187:190	a drug	185:190	a drug in the treatment of hormone-dependent metastatic breast cancer	185:253	Aminoglutethimide (AG) has been used clinically as a drug in the treatment of hormone-dependent metastatic breast cancer.
25434126	1	14	used	used	166:169	arg2	AG					153:154	AG	153:154	AG	153:154	Aminoglutethimide (AG) has been used clinically as a drug in the treatment of hormone-dependent metastatic breast cancer.
25434126	5	15	theme	racemic	709:715	arg1	AG					717:718	racemic AG	709:718	racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol,	709:800	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	6	16	theme	additive	881:888	arg1	monoethanolamine					864:879	monoethanolamine additive	864:888	monoethanolamine additive	864:888	The effects of alcohol content and monoethanolamine additive on the separation performance of racemic AG by HPLC were investigated.
25434126	3	17	theme	high-purity	410:420	arg1	R-					422:423	high-purity R-	410:423	high-purity R-	410:423	Therefore, it was of great significance to obtain the high-purity R-(+)-AG by enantioseparation.
25434126	5	18	theme	AG	717:718	arg1	solubilities					693:704	The solubilities	689:704	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol,	689:800	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	6	19	theme	monoethanolamine	864:879	arg1	effects					833:839	The effects	829:839	The effects of alcohol content and monoethanolamine additive on the separation performance of racemic AG by HPLC	829:940	The effects of alcohol content and monoethanolamine additive on the separation performance of racemic AG by HPLC were investigated.
25434126	7	20	theme	mobile	1077:1082	arg1	n-hexane-ethanol					991:1006	n-hexane-ethanol	991:1006	n-hexane-ethanol (30:70, v/v) with 0.1% monoethanolamine additive	991:1055	According to the experiments, n-hexane-ethanol (30:70, v/v) with 0.1% monoethanolamine additive was selected as the mobile phase.
25434126	7	20	theme	mobile	1077:1082	arg1	phase					1084:1088	the mobile phase	1073:1088	the mobile phase	1073:1088	According to the experiments, n-hexane-ethanol (30:70, v/v) with 0.1% monoethanolamine additive was selected as the mobile phase.
25434126	2	21	theme	S-	277:278	arg1	enantiomer					286:295	S-(-)-AG enantiomer	277:295	S-(-)-AG enantiomer	277:295	It was reported that S-(-)-AG enantiomer had small activity and sometimes might cause side effects.
25434126	7	22	dep	30:70	1009:1013	arg1	v/v					1016:1018	v/v	1016:1018	v/v	1016:1018	According to the experiments, n-hexane-ethanol (30:70, v/v) with 0.1% monoethanolamine additive was selected as the mobile phase.
25434126	3	23	theme	R-	422:423	arg1	-AG					427:429	the high-purity R-(+)-AG	406:429	the high-purity R-(+)-AG by enantioseparation	406:450	Therefore, it was of great significance to obtain the high-purity R-(+)-AG by enantioseparation.
25434126	8	24	theme	theoretical	1154:1164	arg1	plates					1166:1171	theoretical plates	1154:1171	theoretical plates	1154:1171	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	9	25	theme	column	1311:1316	arg1	temperature					1318:1328	column temperature	1311:1328	column temperature	1311:1328	6 mL/min and column temperature of 25-40 °C, with Chiralcel OD-H as stationary phase and n-hexane-ethanol (30:70, v/v) with 0.
25434126	11	26	theme	chromatographic	1506:1520	arg1	separation					1522:1531	chromatographic separation	1506:1531	chromatographic separation	1506:1531	This work provides the basic information of chromatographic separation for the batch and continuous production of aminoglutethimide enantiomers.
25434126	2	27	theme	small	301:305	arg1	activity					307:314	small activity	301:314	small activity	301:314	It was reported that S-(-)-AG enantiomer had small activity and sometimes might cause side effects.
25434126	0	28	theme	[Chromatographic	0:15	arg1	separation					17:26	[Chromatographic separation	0:26	[Chromatographic separation of aminoglutethimide enantiomers on cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase	0:130	[Chromatographic separation of aminoglutethimide enantiomers on cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase].
25434126	4	29	theme	performance	520:530	arg1	HPLC					555:558	HPLC	555:558	HPLC	555:558	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	4	29	theme	performance	520:530	arg1	chromatography					539:552	high performance liquid chromatography	515:552	high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H)	515:686	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	11	30	theme	basic	1485:1489	arg1	information					1491:1501	the basic information	1481:1501	the basic information of chromatographic separation for the batch and continuous production of aminoglutethimide enantiomers	1481:1604	This work provides the basic information of chromatographic separation for the batch and continuous production of aminoglutethimide enantiomers.
25434126	6	31	from	effects	833:839	arg1	performance					908:918	the separation performance	893:918	the separation performance of racemic AG by HPLC	893:940	The effects of alcohol content and monoethanolamine additive on the separation performance of racemic AG by HPLC were investigated.
25434126	11	32	theme	aminoglutethimide	1576:1592	arg1	enantiomers					1594:1604	aminoglutethimide enantiomers	1576:1604	aminoglutethimide enantiomers	1576:1604	This work provides the basic information of chromatographic separation for the batch and continuous production of aminoglutethimide enantiomers.
25434126	4	33	theme	3,5-dimethylphenylcarbamate	625:651	arg1	phase					665:669	cellulose tris(3,5-dimethylphenylcarbamate) stationary phase	610:669	cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H)	610:686	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	4	33	theme	3,5-dimethylphenylcarbamate	625:651	arg1	OD-H					682:685	OD-H	682:685	OD-H	682:685	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	0	34	theme	aminoglutethimide	31:47	arg1	enantiomers					49:59	aminoglutethimide enantiomers	31:59	aminoglutethimide enantiomers	31:59	[Chromatographic separation of aminoglutethimide enantiomers on cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase].
25434126	4	35	theme	high	515:518	arg1	HPLC					555:558	HPLC	555:558	HPLC	555:558	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	4	35	theme	high	515:518	arg1	chromatography					539:552	high performance liquid chromatography	515:552	high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H)	515:686	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	7	36	theme	additive	1048:1055	arg1	monoethanolamine					1031:1046	0.1% monoethanolamine additive	1026:1055	0.1% monoethanolamine additive	1026:1055	According to the experiments, n-hexane-ethanol (30:70, v/v) with 0.1% monoethanolamine additive was selected as the mobile phase.
25434126	8	37	from	flow-rate	1282:1290	arg1	separation					1252:1261	the chromatographic separation	1232:1261	the chromatographic separation of racemic AG at a flow-rate of 0	1232:1295	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	9	38	theme	°C	1339:1340	arg1	mL/min					1300:1305	6 mL/min	1298:1305	6 mL/min	1298:1305	6 mL/min and column temperature of 25-40 °C, with Chiralcel OD-H as stationary phase and n-hexane-ethanol (30:70, v/v) with 0.
25434126	9	38	theme	°C	1339:1340	arg1	temperature					1318:1328	column temperature	1311:1328	column temperature	1311:1328	6 mL/min and column temperature of 25-40 °C, with Chiralcel OD-H as stationary phase and n-hexane-ethanol (30:70, v/v) with 0.
25434126	8	39	theme	chromatographic	1236:1250	arg1	separation					1252:1261	the chromatographic separation	1232:1261	the chromatographic separation of racemic AG at a flow-rate of 0	1232:1295	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	8	40	theme	plates	1166:1171	arg1	resolution					1114:1123	resolution	1114:1123	resolution	1114:1123	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	8	40	theme	plates	1166:1171	arg1	factor					1136:1141	asymmetry factor	1126:1141	asymmetry factor	1126:1141	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	8	40	theme	plates	1166:1171	arg1	factor					1106:1111	The separation factor	1091:1111	The separation factor	1091:1111	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	8	40	theme	plates	1166:1171	arg1	capacity					1192:1199	maximum column capacity	1177:1199	maximum column capacity	1177:1199	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	8	40	theme	plates	1166:1171	arg1	number					1144:1149	number	1144:1149	number of theoretical plates	1144:1171	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	6	41	theme	content	852:858	arg1	effects					833:839	The effects	829:839	The effects of alcohol content and monoethanolamine additive on the separation performance of racemic AG by HPLC	829:940	The effects of alcohol content and monoethanolamine additive on the separation performance of racemic AG by HPLC were investigated.
25434126	2	42	theme	-AG	282:284	arg1	enantiomer					286:295	S-(-)-AG enantiomer	277:295	S-(-)-AG enantiomer	277:295	It was reported that S-(-)-AG enantiomer had small activity and sometimes might cause side effects.
25434126	8	43	theme	column	1185:1190	arg1	capacity					1192:1199	maximum column capacity	1177:1199	maximum column capacity	1177:1199	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	4	44	theme	liquid	532:537	arg1	HPLC					555:558	HPLC	555:558	HPLC	555:558	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	4	44	theme	liquid	532:537	arg1	chromatography					539:552	high performance liquid chromatography	515:552	high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H)	515:686	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	7	45	theme	%	1029:1029	arg1	monoethanolamine					1031:1046	0.1% monoethanolamine additive	1026:1055	0.1% monoethanolamine additive	1026:1055	According to the experiments, n-hexane-ethanol (30:70, v/v) with 0.1% monoethanolamine additive was selected as the mobile phase.
25434126	5	46	from	AG	717:718	arg1	n-hexane/ethanol					759:774	n-hexane/ethanol	759:774	n-hexane/ethanol	759:774	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	5	46	from	AG	717:718	arg1	compositions					745:756	two different solvent compositions	723:756	two different solvent compositions	723:756	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	5	46	from	AG	717:718	arg1	n-hexane/isopropanol					780:799	n-hexane/isopropanol	780:799	n-hexane/isopropanol	780:799	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	9	47	dep	30:70	1405:1409	arg1	v/v					1412:1414	v/v	1412:1414	v/v	1412:1414	6 mL/min and column temperature of 25-40 °C, with Chiralcel OD-H as stationary phase and n-hexane-ethanol (30:70, v/v) with 0.
25434126	4	48	theme	cellulose	610:618	arg1	phase					665:669	cellulose tris(3,5-dimethylphenylcarbamate) stationary phase	610:669	cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H)	610:686	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	4	48	theme	cellulose	610:618	arg1	OD-H					682:685	OD-H	682:685	OD-H	682:685	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	0	49	theme	enantiomers	49:59	arg1	separation					17:26	[Chromatographic separation	0:26	[Chromatographic separation of aminoglutethimide enantiomers on cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase	0:130	[Chromatographic separation of aminoglutethimide enantiomers on cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase].
25434126	8	50	theme	maximum	1177:1183	arg1	capacity					1192:1199	maximum column capacity	1177:1199	maximum column capacity	1177:1199	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	5	51	from	solubilities	693:704	arg1	n-hexane/ethanol					759:774	n-hexane/ethanol	759:774	n-hexane/ethanol	759:774	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	5	51	from	solubilities	693:704	arg1	compositions					745:756	two different solvent compositions	723:756	two different solvent compositions	723:756	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	5	51	from	solubilities	693:704	arg1	n-hexane/isopropanol					780:799	n-hexane/isopropanol	780:799	n-hexane/isopropanol	780:799	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	4	52	theme	aminoglutethimide	467:483	arg1	enantiomers					485:495	aminoglutethimide enantiomers	467:495	aminoglutethimide enantiomers	467:495	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	1	53	theme	hormone-dependent	212:228	arg1	cancer					248:253	hormone-dependent metastatic breast cancer	212:253	hormone-dependent metastatic breast cancer	212:253	Aminoglutethimide (AG) has been used clinically as a drug in the treatment of hormone-dependent metastatic breast cancer.
25434126	9	54	with	n-hexane-ethanol	1387:1402	arg1	0					1422:1422	0	1422:1422	0	1422:1422	6 mL/min and column temperature of 25-40 °C, with Chiralcel OD-H as stationary phase and n-hexane-ethanol (30:70, v/v) with 0.
25434126	8	55	theme	AG	1274:1275	arg1	separation					1252:1261	the chromatographic separation	1232:1261	the chromatographic separation of racemic AG at a flow-rate of 0	1232:1295	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	3	56	theme	great	377:381	arg1	significance					383:394	great significance	377:394	great significance	377:394	Therefore, it was of great significance to obtain the high-purity R-(+)-AG by enantioseparation.
25434126	2	57	contain	had	297:299	arg1	enantiomer					286:295	S-(-)-AG enantiomer	277:295	S-(-)-AG enantiomer	277:295	It was reported that S-(-)-AG enantiomer had small activity and sometimes might cause side effects.
25434126	2	57	contain	had	297:299	arg2	activity					307:314	small activity	301:314	small activity	301:314	It was reported that S-(-)-AG enantiomer had small activity and sometimes might cause side effects.
25434126	7	58	theme	0.1	1026:1028	arg1	%					1029:1029	%	1029:1029	%	1029:1029	According to the experiments, n-hexane-ethanol (30:70, v/v) with 0.1% monoethanolamine additive was selected as the mobile phase.
25434126	6	59	theme	separation	897:906	arg1	performance					908:918	the separation performance	893:918	the separation performance of racemic AG by HPLC	893:940	The effects of alcohol content and monoethanolamine additive on the separation performance of racemic AG by HPLC were investigated.
25434126	11	60	theme	separation	1522:1531	arg1	information					1491:1501	the basic information	1481:1501	the basic information of chromatographic separation for the batch and continuous production of aminoglutethimide enantiomers	1481:1604	This work provides the basic information of chromatographic separation for the batch and continuous production of aminoglutethimide enantiomers.
25434126	8	61	theme	racemic	1266:1272	arg1	AG					1274:1275	racemic AG	1266:1275	racemic AG	1266:1275	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	6	62	theme	racemic	923:929	arg1	AG					931:932	racemic AG	923:932	racemic AG	923:932	The effects of alcohol content and monoethanolamine additive on the separation performance of racemic AG by HPLC were investigated.
25434126	8	63	theme	separation	1095:1104	arg1	factor					1106:1111	The separation factor	1091:1111	The separation factor	1091:1111	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
25434126	1	64	theme	metastatic	230:239	arg1	cancer					248:253	hormone-dependent metastatic breast cancer	212:253	hormone-dependent metastatic breast cancer	212:253	Aminoglutethimide (AG) has been used clinically as a drug in the treatment of hormone-dependent metastatic breast cancer.
25434126	9	65	with	phase	1377:1381	arg1	0					1422:1422	0	1422:1422	0	1422:1422	6 mL/min and column temperature of 25-40 °C, with Chiralcel OD-H as stationary phase and n-hexane-ethanol (30:70, v/v) with 0.
25434126	1	66	from	drug	187:190	arg1	treatment					199:207	the treatment	195:207	the treatment of hormone-dependent metastatic breast cancer	195:253	Aminoglutethimide (AG) has been used clinically as a drug in the treatment of hormone-dependent metastatic breast cancer.
25434126	4	67	theme	stationary	654:663	arg1	phase					665:669	cellulose tris(3,5-dimethylphenylcarbamate) stationary phase	610:669	cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H)	610:686	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	4	67	theme	stationary	654:663	arg1	OD-H					682:685	OD-H	682:685	OD-H	682:685	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	1	68	theme	breast	241:246	arg1	cancer					248:253	hormone-dependent metastatic breast cancer	212:253	hormone-dependent metastatic breast cancer	212:253	Aminoglutethimide (AG) has been used clinically as a drug in the treatment of hormone-dependent metastatic breast cancer.
25434126	6	69	theme	alcohol	844:850	arg1	content					852:858	alcohol content	844:858	alcohol content	844:858	The effects of alcohol content and monoethanolamine additive on the separation performance of racemic AG by HPLC were investigated.
25434126	10	70	theme	mobile	1448:1453	arg1	phase					1455:1459	mobile phase	1448:1459	mobile phase	1448:1459	1% monoethanolamine as mobile phase.
25434126	4	71	theme	tris	620:623	arg1	phase					665:669	cellulose tris(3,5-dimethylphenylcarbamate) stationary phase	610:669	cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H)	610:686	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	4	71	theme	tris	620:623	arg1	OD-H					682:685	OD-H	682:685	OD-H	682:685	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	1	72	theme	cancer	248:253	arg1	treatment					199:207	the treatment	195:207	the treatment of hormone-dependent metastatic breast cancer	195:253	Aminoglutethimide (AG) has been used clinically as a drug in the treatment of hormone-dependent metastatic breast cancer.
25434126	5	73	from	compositions	745:756	arg1	solubilities					693:704	The solubilities	689:704	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol,	689:800	The solubilities of racemic AG in two different solvent compositions, n-hexane/ethanol and n-hexane/isopropanol, were measured, separately.
25434126	4	74	theme	analytical	570:579	arg1	column					581:586	an analytical column	567:586	an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H)	567:686	In this work, aminoglutethimide enantiomers were separated by high performance liquid chromatography (HPLC) using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate) stationary phase (Chiralcel OD-H).
25434126	8	75	theme	asymmetry	1126:1134	arg1	factor					1136:1141	asymmetry factor	1126:1141	asymmetry factor	1126:1141	The separation factor, resolution, asymmetry factor, number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0.
26562555	5	0	theme	loading	759:765	arg1	efficiency					767:776	high drug loading efficiency	749:776	high drug loading efficiency for 5-FU due to the hydrophilic cavities	749:817	The hollow spheres exhibited high drug loading efficiency for 5-FU due to the hydrophilic cavities.
26562555	0	1	theme	drug	87:90	arg1	release					92:98	controlled drug release	76:98	controlled drug release	76:98	Polymeric hollow spheres assembled from ALG-g-PNIPAM and β-cyclodextrin for controlled drug release.
26562555	1	2	theme	sodium	173:178	arg1	N-isopropylacrylamide					200:220	N-isopropylacrylamide	200:220	N-isopropylacrylamide	200:220	In this paper, thermo-sensitive polymeric hollow spheres assembled from sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM) and β-cyclodextrin (β-CD) were prepared for controlled release of 5-fluorouracil (5-FU).
26562555	1	2	theme	sodium	173:178	arg1	ALG-g-PNIPAM					224:235	ALG-g-PNIPAM	224:235	ALG-g-PNIPAM	224:235	In this paper, thermo-sensitive polymeric hollow spheres assembled from sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM) and β-cyclodextrin (β-CD) were prepared for controlled release of 5-fluorouracil (5-FU).
26562555	1	2	theme	sodium	173:178	arg1	alginate-graft-poly					180:198	sodium alginate-graft-poly	173:198	sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM)	173:236	In this paper, thermo-sensitive polymeric hollow spheres assembled from sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM) and β-cyclodextrin (β-CD) were prepared for controlled release of 5-fluorouracil (5-FU).
26562555	5	3	theme	hollow	724:729	arg1	spheres					731:737	The hollow spheres	720:737	The hollow spheres	720:737	The hollow spheres exhibited high drug loading efficiency for 5-FU due to the hydrophilic cavities.
26562555	6	4	theme	significant	882:892	arg1	effect					894:899	a significant effect	880:899	a significant effect	880:899	The initial composition of mixtures, temperature and pH had a significant effect on the inclusion ability and drug release.
26562555	2	5	theme	aqueous	330:336	arg1	solutions					338:346	aqueous solutions	330:346	aqueous solutions	330:346	In aqueous solutions, β-CD and PNIPAM formed rod-like segments through inclusion complexation interactions and sodium alginate acted as coil segments, which resulted in the formation of hollow structures.
26562555	4	6	theme	critical	633:640	arg1	temperature					651:661	The lower critical solution temperature	623:661	The lower critical solution temperature (LCST) of hollow spheres	623:686	The lower critical solution temperature (LCST) of hollow spheres varied in the range of 35-37°C.
26562555	4	6	theme	critical	633:640	arg1	LCST					664:667	LCST	664:667	LCST	664:667	The lower critical solution temperature (LCST) of hollow spheres varied in the range of 35-37°C.
26562555	2	7	theme	complexation	408:419	arg1	interactions					421:432	inclusion complexation interactions	398:432	inclusion complexation interactions	398:432	In aqueous solutions, β-CD and PNIPAM formed rod-like segments through inclusion complexation interactions and sodium alginate acted as coil segments, which resulted in the formation of hollow structures.
26562555	2	8	theme	structures	520:529	arg1	formation					500:508	the formation	496:508	the formation of hollow structures	496:529	In aqueous solutions, β-CD and PNIPAM formed rod-like segments through inclusion complexation interactions and sodium alginate acted as coil segments, which resulted in the formation of hollow structures.
26562555	2	9	theme	coil	463:466	arg1	segments					468:475	coil segments	463:475	coil segments	463:475	In aqueous solutions, β-CD and PNIPAM formed rod-like segments through inclusion complexation interactions and sodium alginate acted as coil segments, which resulted in the formation of hollow structures.
26562555	2	10	theme	inclusion	398:406	arg1	interactions					421:432	inclusion complexation interactions	398:432	inclusion complexation interactions	398:432	In aqueous solutions, β-CD and PNIPAM formed rod-like segments through inclusion complexation interactions and sodium alginate acted as coil segments, which resulted in the formation of hollow structures.
26562555	0	11	theme	hollow	10:15	arg1	spheres					17:23	Polymeric hollow spheres	0:23	Polymeric hollow spheres	0:23	Polymeric hollow spheres assembled from ALG-g-PNIPAM and β-cyclodextrin for controlled drug release.
26562555	0	12	theme	controlled	76:85	arg1	release					92:98	controlled drug release	76:98	controlled drug release	76:98	Polymeric hollow spheres assembled from ALG-g-PNIPAM and β-cyclodextrin for controlled drug release.
26562555	4	13	theme	hollow	673:678	arg1	spheres					680:686	hollow spheres	673:686	hollow spheres	673:686	The lower critical solution temperature (LCST) of hollow spheres varied in the range of 35-37°C.
26562555	0	14	theme	Polymeric	0:8	arg1	spheres					17:23	Polymeric hollow spheres	0:23	Polymeric hollow spheres	0:23	Polymeric hollow spheres assembled from ALG-g-PNIPAM and β-cyclodextrin for controlled drug release.
26562555	4	15	theme	35-37°C	711:717	arg1	range					702:706	the range	698:706	the range of 35-37°C	698:717	The lower critical solution temperature (LCST) of hollow spheres varied in the range of 35-37°C.
26562555	3	16	from	increase	593:600	arg1	mixtures					613:620	mixtures	613:620	mixtures	613:620	The size and wall thickness of assemblies increased with the increase of β-CD in mixtures.
26562555	5	17	theme	hydrophilic	798:808	arg1	cavities					810:817	the hydrophilic cavities	794:817	the hydrophilic cavities	794:817	The hollow spheres exhibited high drug loading efficiency for 5-FU due to the hydrophilic cavities.
26562555	3	18	theme	assemblies	563:572	arg1	thickness					550:558	wall thickness	545:558	wall thickness	545:558	The size and wall thickness of assemblies increased with the increase of β-CD in mixtures.
26562555	3	18	theme	assemblies	563:572	arg1	size					536:539	size	536:539	size	536:539	The size and wall thickness of assemblies increased with the increase of β-CD in mixtures.
26562555	4	19	theme	solution	642:649	arg1	temperature					651:661	The lower critical solution temperature	623:661	The lower critical solution temperature (LCST) of hollow spheres	623:686	The lower critical solution temperature (LCST) of hollow spheres varied in the range of 35-37°C.
26562555	4	19	theme	solution	642:649	arg1	LCST					664:667	LCST	664:667	LCST	664:667	The lower critical solution temperature (LCST) of hollow spheres varied in the range of 35-37°C.
26562555	1	20	theme	5-fluorouracil	304:317	arg1	release					293:299	controlled release	282:299	controlled release of 5-fluorouracil (5-FU)	282:324	In this paper, thermo-sensitive polymeric hollow spheres assembled from sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM) and β-cyclodextrin (β-CD) were prepared for controlled release of 5-fluorouracil (5-FU).
26562555	3	21	dep	size	536:539	arg1	The					532:534	The	532:534	The	532:534	The size and wall thickness of assemblies increased with the increase of β-CD in mixtures.
26562555	2	22	theme	sodium	438:443	arg1	alginate					445:452	sodium alginate	438:452	sodium alginate	438:452	In aqueous solutions, β-CD and PNIPAM formed rod-like segments through inclusion complexation interactions and sodium alginate acted as coil segments, which resulted in the formation of hollow structures.
26562555	7	23	theme	release	1035:1041	arg1	rate					1043:1046	a more rapid release rate	1022:1046	a more rapid release rate	1022:1046	Increasing temperatures above the LCST or decreasing pH to acidic conditions, a more rapid release rate was observed.
26562555	6	24	dep	ability	918:924	arg1	the					904:906	the	904:906	the	904:906	The initial composition of mixtures, temperature and pH had a significant effect on the inclusion ability and drug release.
26562555	3	25	theme	β-CD	605:608	arg1	increase					593:600	the increase	589:600	the increase of β-CD in mixtures	589:620	The size and wall thickness of assemblies increased with the increase of β-CD in mixtures.
26562555	5	26	theme	high	749:752	arg1	efficiency					767:776	high drug loading efficiency	749:776	high drug loading efficiency for 5-FU due to the hydrophilic cavities	749:817	The hollow spheres exhibited high drug loading efficiency for 5-FU due to the hydrophilic cavities.
26562555	7	27	theme	acidic	1003:1008	arg1	conditions					1010:1019	acidic conditions	1003:1019	acidic conditions	1003:1019	Increasing temperatures above the LCST or decreasing pH to acidic conditions, a more rapid release rate was observed.
26562555	6	28	contain	had	876:878	arg1	composition					832:842	The initial composition	820:842	The initial composition of mixtures, temperature and pH	820:874	The initial composition of mixtures, temperature and pH had a significant effect on the inclusion ability and drug release.
26562555	6	28	contain	had	876:878	arg2	effect					894:899	a significant effect	880:899	a significant effect	880:899	The initial composition of mixtures, temperature and pH had a significant effect on the inclusion ability and drug release.
26562555	6	29	theme	drug	930:933	arg1	release					935:941	drug release	930:941	drug release	930:941	The initial composition of mixtures, temperature and pH had a significant effect on the inclusion ability and drug release.
26562555	6	30	theme	mixtures	847:854	arg1	composition					832:842	The initial composition	820:842	The initial composition of mixtures, temperature and pH	820:874	The initial composition of mixtures, temperature and pH had a significant effect on the inclusion ability and drug release.
26562555	7	31	theme	rapid	1029:1033	arg1	rate					1043:1046	a more rapid release rate	1022:1046	a more rapid release rate	1022:1046	Increasing temperatures above the LCST or decreasing pH to acidic conditions, a more rapid release rate was observed.
26562555	1	32	theme	thermo-sensitive	116:131	arg1	spheres					150:156	thermo-sensitive polymeric hollow spheres	116:156	thermo-sensitive polymeric hollow spheres assembled from sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM) and β-cyclodextrin (β-CD)	116:262	In this paper, thermo-sensitive polymeric hollow spheres assembled from sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM) and β-cyclodextrin (β-CD) were prepared for controlled release of 5-fluorouracil (5-FU).
26562555	1	33	theme	polymeric	133:141	arg1	spheres					150:156	thermo-sensitive polymeric hollow spheres	116:156	thermo-sensitive polymeric hollow spheres assembled from sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM) and β-cyclodextrin (β-CD)	116:262	In this paper, thermo-sensitive polymeric hollow spheres assembled from sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM) and β-cyclodextrin (β-CD) were prepared for controlled release of 5-fluorouracil (5-FU).
26562555	3	34	theme	wall	545:548	arg1	thickness					550:558	wall thickness	545:558	wall thickness	545:558	The size and wall thickness of assemblies increased with the increase of β-CD in mixtures.
26562555	6	35	theme	initial	824:830	arg1	composition					832:842	The initial composition	820:842	The initial composition of mixtures, temperature and pH	820:874	The initial composition of mixtures, temperature and pH had a significant effect on the inclusion ability and drug release.
26562555	1	36	theme	hollow	143:148	arg1	spheres					150:156	thermo-sensitive polymeric hollow spheres	116:156	thermo-sensitive polymeric hollow spheres assembled from sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM) and β-cyclodextrin (β-CD)	116:262	In this paper, thermo-sensitive polymeric hollow spheres assembled from sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM) and β-cyclodextrin (β-CD) were prepared for controlled release of 5-fluorouracil (5-FU).
26562555	1	37	theme	controlled	282:291	arg1	release					293:299	controlled release	282:299	controlled release of 5-fluorouracil (5-FU)	282:324	In this paper, thermo-sensitive polymeric hollow spheres assembled from sodium alginate-graft-poly(N-isopropylacrylamide) (ALG-g-PNIPAM) and β-cyclodextrin (β-CD) were prepared for controlled release of 5-fluorouracil (5-FU).
26562555	2	38	theme	hollow	513:518	arg1	structures					520:529	hollow structures	513:529	hollow structures	513:529	In aqueous solutions, β-CD and PNIPAM formed rod-like segments through inclusion complexation interactions and sodium alginate acted as coil segments, which resulted in the formation of hollow structures.
26562555	2	39	theme	rod-like	372:379	arg1	segments					381:388	rod-like segments	372:388	rod-like segments	372:388	In aqueous solutions, β-CD and PNIPAM formed rod-like segments through inclusion complexation interactions and sodium alginate acted as coil segments, which resulted in the formation of hollow structures.
26562555	5	40	theme	drug	754:757	arg1	efficiency					767:776	high drug loading efficiency	749:776	high drug loading efficiency for 5-FU due to the hydrophilic cavities	749:817	The hollow spheres exhibited high drug loading efficiency for 5-FU due to the hydrophilic cavities.
26562555	6	41	theme	inclusion	908:916	arg1	ability					918:924	inclusion ability	908:924	inclusion ability	908:924	The initial composition of mixtures, temperature and pH had a significant effect on the inclusion ability and drug release.
26562555	4	42	theme	spheres	680:686	arg1	temperature					651:661	The lower critical solution temperature	623:661	The lower critical solution temperature (LCST) of hollow spheres	623:686	The lower critical solution temperature (LCST) of hollow spheres varied in the range of 35-37°C.
26562555	4	42	theme	spheres	680:686	arg1	LCST					664:667	LCST	664:667	LCST	664:667	The lower critical solution temperature (LCST) of hollow spheres varied in the range of 35-37°C.
28330754	4	0	theme	CS/GP	919:923	arg1	hydrogels					925:933	CS/GP hydrogels	919:933	CS/GP hydrogels	919:933	The SDF-1α encapsulated in CS/CMCS NPs within CS/GP hydrogels showed significantly sustained release effect.
28330754	1	1	theme	skeleton	271:278	arg1	anatomy					243:249	the delicate and complex anatomy	218:249	the delicate and complex anatomy of the craniofacial skeleton	218:278	Repair of craniofacial bony defects remains a challenge for surgeons due to the delicate and complex anatomy of the craniofacial skeleton.
28330754	2	2	theme	easy	476:479	arg1	degradation					481:491	easy degradation	476:491	easy degradation	476:491	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	0	3	theme	bone	123:126	arg1	regeneration					128:139	calvarial bone regeneration	113:139	calvarial bone regeneration	113:139	Injectable nanoparticles/hydrogels composite as sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration.
28330754	6	4	theme	embedded	1239:1246	arg1	group					1258:1262	the SDF-1α embedded hydrogels group	1228:1262	the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05)	1228:1283	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	6	5	theme	control	1293:1299	arg1	group					1301:1305	the control group	1289:1305	the control group (8.64±4.8%, p<0.01)	1289:1325	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	8	6	theme	injectable	1448:1457	arg1	hydrogels					1459:1467	nanoparticulate injectable hydrogels	1432:1467	nanoparticulate injectable hydrogels	1432:1467	This study demonstrated the potential applications of nanoparticulate injectable hydrogels for sustained release SDF-1α on bone tissue regeneration.
28330754	8	7	theme	hydrogels	1459:1467	arg1	applications					1416:1427	the potential applications	1402:1427	the potential applications of nanoparticulate injectable hydrogels for sustained release SDF-1α on bone tissue regeneration	1402:1524	This study demonstrated the potential applications of nanoparticulate injectable hydrogels for sustained release SDF-1α on bone tissue regeneration.
28330754	2	8	link	cell-derived	289:300	arg1	SDF-1α					313:318	SDF-1α	313:318	SDF-1α	313:318	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	2	8	link	cell-derived	289:300	arg1	factor-1α					302:310	Stromal cell-derived factor-1α	281:310	Stromal cell-derived factor-1α (SDF-1α)	281:319	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	2	8	link	cell-derived	289:300	arg1	chemokine					337:345	an important chemokine	324:345	an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy	324:537	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	0	9	theme	calvarial	113:121	arg1	regeneration					128:139	calvarial bone regeneration	113:139	calvarial bone regeneration	113:139	Injectable nanoparticles/hydrogels composite as sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration.
28330754	6	10	theme	new	1176:1178	arg1	formation					1185:1193	the new bone formation	1172:1193	the new bone formation (38.5±4.5%)	1172:1205	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	6	10	theme	new	1176:1178	arg1	%					1204:1204	38.5±4.5%	1196:1204	38.5±4.5%	1196:1204	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	6	11	theme	embedded	1119:1126	arg1	group					1138:1142	the SDF-1α/CS/CMCS NPs embedded hydrogels group	1096:1142	the SDF-1α/CS/CMCS NPs embedded hydrogels group	1096:1142	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	2	12	theme	cells	409:413	arg1	homing					382:387	the homing	378:387	the homing of mesenchymal stem cells (MSC)	378:419	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	6	13	dep	group	1301:1305	arg1	p<0.01					1319:1324	p<0.01	1319:1324	p<0.01	1319:1324	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	6	13	dep	group	1301:1305	arg1	%					1316:1316	8.64±4.8%	1308:1316	8.64±4.8%	1308:1316	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	6	14	theme	bone	1180:1183	arg1	formation					1185:1193	the new bone formation	1172:1193	the new bone formation (38.5±4.5%)	1172:1205	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	6	14	theme	bone	1180:1183	arg1	%					1204:1204	38.5±4.5%	1196:1204	38.5±4.5%	1196:1204	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	6	15	theme	defects	1068:1074	arg1	model					1076:1080	rat calvarial defects model	1054:1080	rat calvarial defects model	1054:1080	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	2	16	theme	stem	404:407	arg1	MSC					416:418	MSC	416:418	MSC	416:418	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	2	16	theme	stem	404:407	arg1	cells					409:413	mesenchymal stem cells	392:413	mesenchymal stem cells (MSC)	392:419	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	3	17	theme	loading	738:744	arg1	efficiency					746:755	loading efficiency	738:755	loading efficiency	738:755	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	2	18	theme	mesenchymal	392:402	arg1	MSC					416:418	MSC	416:418	MSC	416:418	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	2	18	theme	mesenchymal	392:402	arg1	cells					409:413	mesenchymal stem cells	392:413	mesenchymal stem cells (MSC)	392:419	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	0	19	theme	Injectable	0:9	arg1	nanoparticles/hydrogels					11:33	Injectable nanoparticles/hydrogels	0:33	Injectable nanoparticles/hydrogels composite as sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration.	0:140	Injectable nanoparticles/hydrogels composite as sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration.
28330754	4	20	theme	CS/CMCS	900:906	arg1	NPs					908:910	CS/CMCS NPs	900:910	CS/CMCS NPs	900:910	The SDF-1α encapsulated in CS/CMCS NPs within CS/GP hydrogels showed significantly sustained release effect.
28330754	2	21	theme	important	327:335	arg1	factor-1α					302:310	Stromal cell-derived factor-1α	281:310	Stromal cell-derived factor-1α (SDF-1α)	281:319	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	2	21	theme	important	327:335	arg1	chemokine					337:345	an important chemokine	324:345	an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy	324:537	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	8	22	theme	nanoparticulate	1432:1446	arg1	hydrogels					1459:1467	nanoparticulate injectable hydrogels	1432:1467	nanoparticulate injectable hydrogels	1432:1467	This study demonstrated the potential applications of nanoparticulate injectable hydrogels for sustained release SDF-1α on bone tissue regeneration.
28330754	3	23	from	hydrogels	862:870	arg1	characteristics					773:787	the release characteristics	761:787	the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	761:870	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	23	from	hydrogels	862:870	arg1	morphology					695:704	morphology	695:704	morphology	695:704	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	23	from	hydrogels	862:870	arg1	size					716:719	particle size	707:719	particle size	707:719	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	23	from	hydrogels	862:870	arg1	potential					727:735	zeta potential	722:735	zeta potential	722:735	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	23	from	hydrogels	862:870	arg1	efficiency					746:755	loading efficiency	738:755	loading efficiency	738:755	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	8	24	theme	potential	1406:1414	arg1	applications					1416:1427	the potential applications	1402:1427	the potential applications of nanoparticulate injectable hydrogels for sustained release SDF-1α on bone tissue regeneration	1402:1524	This study demonstrated the potential applications of nanoparticulate injectable hydrogels for sustained release SDF-1α on bone tissue regeneration.
28330754	3	25	theme	SDF-1α/chitosan/carboxymeymethy-chitosan	554:593	arg1	nanoparticles					595:607	SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles	554:607	SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs)	554:628	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	25	theme	SDF-1α/chitosan/carboxymeymethy-chitosan	554:593	arg1	NPs					625:627	SDF-1α/CS/CMCS NPs	610:627	SDF-1α/CS/CMCS NPs	610:627	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	5	26	theme	SDF-1α	1008:1013	arg1	%					1026:1026	only 40%	1019:1026	only 40%	1019:1026	The cumulative release of SDF-1α was only 40% during 28d.
28330754	5	26	theme	SDF-1α	1008:1013	arg1	release					997:1003	The cumulative release	982:1003	The cumulative release of SDF-1α	982:1013	The cumulative release of SDF-1α was only 40% during 28d.
28330754	3	27	theme	various	666:672	arg1	efficiency					746:755	loading efficiency	738:755	loading efficiency	738:755	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	27	theme	various	666:672	arg1	size					716:719	particle size	707:719	particle size	707:719	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	27	theme	various	666:672	arg1	parameters					674:683	various parameters	666:683	various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	666:870	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	27	theme	various	666:672	arg1	potential					727:735	zeta potential	722:735	zeta potential	722:735	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	27	theme	various	666:672	arg1	characteristics					773:787	the release characteristics	761:787	the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	761:870	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	27	theme	various	666:672	arg1	morphology					695:704	morphology	695:704	morphology	695:704	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	1	28	theme	delicate	222:229	arg1	anatomy					243:249	the delicate and complex anatomy	218:249	the delicate and complex anatomy of the craniofacial skeleton	218:278	Repair of craniofacial bony defects remains a challenge for surgeons due to the delicate and complex anatomy of the craniofacial skeleton.
28330754	0	29	theme	release	58:64	arg1	system					66:71	sustained release system	48:71	sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration	48:139	Injectable nanoparticles/hydrogels composite as sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration.
28330754	3	30	theme	release	765:771	arg1	characteristics					773:787	the release characteristics	761:787	the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	761:870	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	8	31	theme	sustained	1473:1481	arg1	release					1483:1489	sustained release	1473:1489	sustained release SDF-1α on bone tissue regeneration	1473:1524	This study demonstrated the potential applications of nanoparticulate injectable hydrogels for sustained release SDF-1α on bone tissue regeneration.
28330754	3	32	theme	SDF-1α/CS/CMCS	610:623	arg1	nanoparticles					595:607	SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles	554:607	SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs)	554:628	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	32	theme	SDF-1α/CS/CMCS	610:623	arg1	NPs					625:627	SDF-1α/CS/CMCS NPs	610:627	SDF-1α/CS/CMCS NPs	610:627	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	0	33	theme	sustained	48:56	arg1	system					66:71	sustained release system	48:71	sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration	48:139	Injectable nanoparticles/hydrogels composite as sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration.
28330754	3	34	theme	phosphate	830:838	arg1	hydrogels					862:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	2	35	theme	low	526:528	arg1	efficacy					530:537	relatively low efficacy	515:537	relatively low efficacy	515:537	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	7	36	theme	Histological	1328:1339	arg1	data					1341:1344	Histological data	1328:1344	Histological data	1328:1344	Histological data also confirmed this difference.
28330754	6	37	theme	calvarial	1058:1066	arg1	model					1076:1080	rat calvarial defects model	1054:1080	rat calvarial defects model	1054:1080	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	1	38	theme	complex	235:241	arg1	anatomy					243:249	the delicate and complex anatomy	218:249	the delicate and complex anatomy of the craniofacial skeleton	218:278	Repair of craniofacial bony defects remains a challenge for surgeons due to the delicate and complex anatomy of the craniofacial skeleton.
28330754	2	39	theme	short	456:460	arg1	half-life					462:470	short half-life	456:470	short half-life	456:470	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	3	40	theme	disodium	840:847	arg1	hydrogels					862:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	4	41	theme	sustained	956:964	arg1	effect					974:979	sustained release effect	956:979	sustained release effect	956:979	The SDF-1α encapsulated in CS/CMCS NPs within CS/GP hydrogels showed significantly sustained release effect.
28330754	6	42	dep	group	1258:1262	arg1	p<0.05					1277:1282	p<0.05	1277:1282	p<0.05	1277:1282	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	6	42	dep	group	1258:1262	arg1	%					1274:1274	26.3±7.25%	1265:1274	26.3±7.25%	1265:1274	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	2	43	theme	critical	361:368	arg1	role					370:373	a critical role	359:373	a critical role	359:373	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	3	44	theme	thermosensitive	794:808	arg1	hydrogels					862:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	8	45	theme	tissue	1506:1511	arg1	regeneration					1513:1524	bone tissue regeneration	1501:1524	bone tissue regeneration	1501:1524	This study demonstrated the potential applications of nanoparticulate injectable hydrogels for sustained release SDF-1α on bone tissue regeneration.
28330754	8	46	theme	bone	1501:1504	arg1	regeneration					1513:1524	bone tissue regeneration	1501:1524	bone tissue regeneration	1501:1524	This study demonstrated the potential applications of nanoparticulate injectable hydrogels for sustained release SDF-1α on bone tissue regeneration.
28330754	0	47	link	cell-derived	86:97	arg1	factor-1α					99:107	stromal cell-derived factor-1α	78:107	stromal cell-derived factor-1α for calvarial bone regeneration	78:139	Injectable nanoparticles/hydrogels composite as sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration.
28330754	6	48	from	model	1076:1080	arg1	data					1044:1047	The data	1040:1047	The data from rat calvarial defects model	1040:1080	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	3	49	theme	chitosan/β-glycerol	810:828	arg1	hydrogels					862:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	3	50	theme	particle	707:714	arg1	size					716:719	particle size	707:719	particle size	707:719	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	2	51	theme	cell-derived	289:300	arg1	SDF-1α					313:318	SDF-1α	313:318	SDF-1α	313:318	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	2	51	theme	cell-derived	289:300	arg1	factor-1α					302:310	Stromal cell-derived factor-1α	281:310	Stromal cell-derived factor-1α (SDF-1α)	281:319	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	2	51	theme	cell-derived	289:300	arg1	chemokine					337:345	an important chemokine	324:345	an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy	324:537	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	1	52	theme	craniofacial	152:163	arg1	defects					170:176	craniofacial bony defects	152:176	craniofacial bony defects	152:176	Repair of craniofacial bony defects remains a challenge for surgeons due to the delicate and complex anatomy of the craniofacial skeleton.
28330754	3	53	theme	CS/GP	855:859	arg1	hydrogels					862:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	4	54	theme	release	966:972	arg1	effect					974:979	sustained release effect	956:979	sustained release effect	956:979	The SDF-1α encapsulated in CS/CMCS NPs within CS/GP hydrogels showed significantly sustained release effect.
28330754	2	55	theme	Stromal	281:287	arg1	SDF-1α					313:318	SDF-1α	313:318	SDF-1α	313:318	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	2	55	theme	Stromal	281:287	arg1	factor-1α					302:310	Stromal cell-derived factor-1α	281:310	Stromal cell-derived factor-1α (SDF-1α)	281:319	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	2	55	theme	Stromal	281:287	arg1	chemokine					337:345	an important chemokine	324:345	an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy	324:537	Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy.
28330754	1	56	theme	bony	165:168	arg1	defects					170:176	craniofacial bony defects	152:176	craniofacial bony defects	152:176	Repair of craniofacial bony defects remains a challenge for surgeons due to the delicate and complex anatomy of the craniofacial skeleton.
28330754	5	57	theme	cumulative	986:995	arg1	%					1026:1026	only 40%	1019:1026	only 40%	1019:1026	The cumulative release of SDF-1α was only 40% during 28d.
28330754	5	57	theme	cumulative	986:995	arg1	release					997:1003	The cumulative release	982:1003	The cumulative release of SDF-1α	982:1013	The cumulative release of SDF-1α was only 40% during 28d.
28330754	0	58	theme	cell-derived	86:97	arg1	factor-1α					99:107	stromal cell-derived factor-1α	78:107	stromal cell-derived factor-1α for calvarial bone regeneration	78:139	Injectable nanoparticles/hydrogels composite as sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration.
28330754	6	59	theme	SDF-1α	1232:1237	arg1	group					1258:1262	the SDF-1α embedded hydrogels group	1228:1262	the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05)	1228:1283	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	0	60	with	system	66:71	arg1	factor-1α					99:107	stromal cell-derived factor-1α	78:107	stromal cell-derived factor-1α for calvarial bone regeneration	78:139	Injectable nanoparticles/hydrogels composite as sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration.
28330754	1	61	theme	defects	170:176	arg1	Repair					142:147	Repair	142:147	Repair of craniofacial bony defects	142:176	Repair of craniofacial bony defects remains a challenge for surgeons due to the delicate and complex anatomy of the craniofacial skeleton.
28330754	6	62	theme	hydrogels	1128:1136	arg1	group					1138:1142	the SDF-1α/CS/CMCS NPs embedded hydrogels group	1096:1142	the SDF-1α/CS/CMCS NPs embedded hydrogels group	1096:1142	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	3	63	theme	salt	849:852	arg1	hydrogels					862:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels	794:870	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	0	64	theme	stromal	78:84	arg1	factor-1α					99:107	stromal cell-derived factor-1α	78:107	stromal cell-derived factor-1α for calvarial bone regeneration	78:139	Injectable nanoparticles/hydrogels composite as sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration.
28330754	3	65	theme	zeta	722:725	arg1	potential					727:735	zeta potential	722:735	zeta potential	722:735	In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels.
28330754	6	66	theme	rat	1054:1056	arg1	model					1076:1080	rat calvarial defects model	1054:1080	rat calvarial defects model	1054:1080	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	6	67	theme	hydrogels	1248:1256	arg1	group					1258:1262	the SDF-1α embedded hydrogels group	1228:1262	the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05)	1228:1283	The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01).
28330754	1	68	theme	craniofacial	258:269	arg1	skeleton					271:278	the craniofacial skeleton	254:278	the craniofacial skeleton	254:278	Repair of craniofacial bony defects remains a challenge for surgeons due to the delicate and complex anatomy of the craniofacial skeleton.
26890795	4	0	theme	solvent-based	453:465	arg1	fractionation					482:494	organic solvent-based lignocellulose fractionation	445:494	organic solvent-based lignocellulose fractionation	445:494	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	1	1	theme	tree	133:136	arg1	legume					138:143	An efficient biomass producing tree legume	102:143	An efficient biomass producing tree legume	102:143	H.S. Irwin & Barneby: An efficient biomass producing tree legume.
26890795	4	2	theme	glucan	395:400	arg1	hydrolysis					377:386	the hydrolysis	373:386	the hydrolysis of the glucan	373:400	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	6	3	theme	pretreated	966:975	arg1	biomass					1010:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.
26890795	5	4	theme	glucose	942:948	arg1	release					950:956	the maximum glucose release	930:956	the maximum glucose release	930:956	Of the biomass of different particle sizes (40-200mesh) used for saccharification, 40-60mesh shown the maximum glucose release.
26890795	3	5	theme	1year	321:325	arg1	Composition					298:308	Composition	298:308	Composition of mature, 1year and 2years coppice biomass	298:352	Composition of mature, 1year and 2years coppice biomass were analysed.
26890795	4	6	theme	glucan	823:828	arg1	gram					815:818	gram	815:818	gram of glucan	815:828	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	6	7	theme	glucose	1036:1042	arg1	profiles					1052:1059	equivalent glucose release profiles	1025:1059	equivalent glucose release profiles	1025:1059	COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.
26890795	4	8	theme	international	769:781	arg1	use					715:717	the use	711:717	the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan	711:828	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	9	dep	exhibited	627:635	arg1	result					642:647	result	642:647	exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan	627:828	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	1	10	theme	efficient	105:113	arg1	legume					138:143	An efficient biomass producing tree legume	102:143	An efficient biomass producing tree legume	102:143	H.S. Irwin & Barneby: An efficient biomass producing tree legume.
26890795	3	11	theme	mature	313:318	arg1	Composition					298:308	Composition	298:308	Composition of mature, 1year and 2years coppice biomass	298:352	Composition of mature, 1year and 2years coppice biomass were analysed.
26890795	4	12	theme	%	663:663	arg1	glucose					665:671	88.90% glucose	658:671	88.90% glucose	658:671	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	13	dep	acid	569:572	arg1	incubation					581:590	45min incubation	575:590	45min incubation at 50°C	575:598	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	6	14	theme	mature	977:982	arg1	biomass					1010:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.
26890795	5	15	theme	different	849:857	arg1	40-200mesh					875:884	40-200mesh	875:884	40-200mesh	875:884	Of the biomass of different particle sizes (40-200mesh) used for saccharification, 40-60mesh shown the maximum glucose release.
26890795	5	15	theme	different	849:857	arg1	sizes					868:872	different particle sizes	849:872	different particle sizes (40-200mesh) used for saccharification	849:911	Of the biomass of different particle sizes (40-200mesh) used for saccharification, 40-60mesh shown the maximum glucose release.
26890795	6	16	theme	COSLIF	959:964	arg1	biomass					1010:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.
26890795	4	17	theme	organic	445:451	arg1	fractionation					482:494	organic solvent-based lignocellulose fractionation	445:494	organic solvent-based lignocellulose fractionation	445:494	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	18	theme	incubation	695:704	arg1	72h					688:690	72h	688:690	72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan	688:828	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	2	19	from	biomass	213:219	arg1	release					185:191	efficient release	175:191	efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes	175:295	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	6	20	theme	release	1044:1050	arg1	profiles					1052:1059	equivalent glucose release profiles	1025:1059	equivalent glucose release profiles	1025:1059	COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.
26890795	4	21	theme	cellulase	752:760	arg1	FPU					744:746	FPU	744:746	FPU	744:746	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	21	theme	cellulase	752:760	arg1	units					737:741	5 filter paper units	722:741	5 filter paper units (FPU) of cellulase	722:760	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	21	theme	cellulase	752:760	arg1	IU					790:791	IU	790:791	IU	790:791	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	2	22	theme	producing	274:282	arg1	legumes					289:295	the highly efficient biomass producing tree legumes	245:295	the highly efficient biomass producing tree legumes	245:295	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	2	23	theme	tree	284:287	arg1	legumes					289:295	the highly efficient biomass producing tree legumes	245:295	the highly efficient biomass producing tree legumes	245:295	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	4	24	theme	cellulose	422:430	arg1	COSLIF					497:502	COSLIF	497:502	COSLIF	497:502	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	24	theme	cellulose	422:430	arg1	solvent-					432:439	cellulose solvent-	422:439	cellulose solvent-	422:439	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	0	25	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, pretreatments and saccharification of Senna siamea (Lam.)
26890795	4	26	theme	sodium	517:522	arg1	alkali					509:514	alkali	509:514	alkali (sodium hydroxide)	509:533	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	26	theme	sodium	517:522	arg1	hydroxide					524:532	sodium hydroxide	517:532	sodium hydroxide	517:532	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	1	27	dep	legume	138:143	arg1	Barneby					93:99	Barneby	93:99	Barneby	93:99	H.S. Irwin & Barneby: An efficient biomass producing tree legume.
26890795	5	28	theme	particle	859:866	arg1	40-200mesh					875:884	40-200mesh	875:884	40-200mesh	875:884	Of the biomass of different particle sizes (40-200mesh) used for saccharification, 40-60mesh shown the maximum glucose release.
26890795	5	28	theme	particle	859:866	arg1	sizes					868:872	different particle sizes	849:872	different particle sizes (40-200mesh) used for saccharification	849:911	Of the biomass of different particle sizes (40-200mesh) used for saccharification, 40-60mesh shown the maximum glucose release.
26890795	3	29	theme	2years	331:336	arg1	Composition					298:308	Composition	298:308	Composition of mature, 1year and 2years coppice biomass	298:352	Composition of mature, 1year and 2years coppice biomass were analysed.
26890795	0	30	dep	composition	9:19	arg1	Lam					74:76	Lam	74:76	Lam.	74:77	Chemical composition, pretreatments and saccharification of Senna siamea (Lam.)
26890795	4	31	theme	units	737:741	arg1	use					715:717	the use	711:717	the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan	711:828	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	2	32	theme	biomass	266:272	arg1	legumes					289:295	the highly efficient biomass producing tree legumes	245:295	the highly efficient biomass producing tree legumes	245:295	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	3	33	theme	coppice	338:344	arg1	biomass					346:352	coppice biomass	338:352	coppice biomass	338:352	Composition of mature, 1year and 2years coppice biomass were analysed.
26890795	4	34	theme	β-glucosidase	797:809	arg1	FPU					744:746	FPU	744:746	FPU	744:746	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	34	theme	β-glucosidase	797:809	arg1	units					737:741	5 filter paper units	722:741	5 filter paper units (FPU) of cellulase	722:760	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	34	theme	β-glucosidase	797:809	arg1	IU					790:791	IU	790:791	IU	790:791	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	35	dep	COSLIF	546:551	arg1	acid					569:572	85% phosphoric acid	554:572	85% phosphoric acid	554:572	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	2	36	theme	efficient	256:264	arg1	legumes					289:295	the highly efficient biomass producing tree legumes	245:295	the highly efficient biomass producing tree legumes	245:295	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	4	37	dep	units	737:741	arg1	units					783:787	units	783:787	units	783:787	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	38	theme	paper	731:735	arg1	FPU					744:746	FPU	744:746	FPU	744:746	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	38	theme	paper	731:735	arg1	units					737:741	5 filter paper units	722:741	5 filter paper units (FPU) of cellulase	722:760	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	38	theme	paper	731:735	arg1	IU					790:791	IU	790:791	IU	790:791	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	39	theme	filter	724:729	arg1	FPU					744:746	FPU	744:746	FPU	744:746	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	39	theme	filter	724:729	arg1	units					737:741	5 filter paper units	722:741	5 filter paper units (FPU) of cellulase	722:760	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	39	theme	filter	724:729	arg1	IU					790:791	IU	790:791	IU	790:791	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	40	theme	45min	575:579	arg1	incubation					581:590	45min incubation	575:590	45min incubation at 50°C	575:598	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	0	41	theme	Senna	60:64	arg1	siamea					66:71	Senna siamea	60:71	Senna siamea	60:71	Chemical composition, pretreatments and saccharification of Senna siamea (Lam.)
26890795	3	42	dep	mature	313:318	arg1	biomass					346:352	coppice biomass	338:352	coppice biomass	338:352	Composition of mature, 1year and 2years coppice biomass were analysed.
26890795	4	43	with	incubation	695:704	arg1	use					715:717	the use	711:717	the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan	711:828	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	6	44	theme	1year	985:989	arg1	biomass					1010:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.
26890795	2	45	theme	glucose	196:202	arg1	release					185:191	efficient release	175:191	efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes	175:295	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	6	46	theme	coppice	1002:1008	arg1	biomass					1010:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.
26890795	5	47	theme	sizes	868:872	arg1	biomass					838:844	the biomass	834:844	the biomass of different particle sizes (40-200mesh) used for saccharification	834:911	Of the biomass of different particle sizes (40-200mesh) used for saccharification, 40-60mesh shown the maximum glucose release.
26890795	4	48	theme	mature	612:617	arg1	biomass					619:625	mature biomass	612:625	mature biomass	612:625	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	6	49	theme	2years	995:1000	arg1	biomass					1010:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass	959:1016	COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.
26890795	6	50	theme	equivalent	1025:1034	arg1	profiles					1052:1059	equivalent glucose release profiles	1025:1059	equivalent glucose release profiles	1025:1059	COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.
26890795	4	51	used	used	540:543	arg2	COSLIF					497:502	COSLIF	497:502	COSLIF	497:502	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	51	used	used	540:543	arg2	pretreatments					407:419	two pretreatments	403:419	two pretreatments	403:419	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	51	used	used	540:543	arg2	alkali					509:514	alkali	509:514	alkali (sodium hydroxide)	509:533	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	51	used	used	540:543	arg2	solvent-					432:439	cellulose solvent-	422:439	cellulose solvent-	422:439	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	4	51	used	used	540:543	arg2	hydroxide					524:532	sodium hydroxide	517:532	sodium hydroxide	517:532	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	2	52	theme	efficient	175:183	arg1	release					185:191	efficient release	175:191	efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes	175:295	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	2	53	theme	siamea	230:235	arg1	biomass					213:219	the biomass	209:219	the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes	209:295	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	2	54	theme	legumes	289:295	arg1	one					238:240	one	238:240	one	238:240	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	2	54	theme	legumes	289:295	arg1	siamea					230:235	Senna siamea	224:235	Senna siamea	224:235	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	2	54	theme	legumes	289:295	arg1	legumes					289:295	the highly efficient biomass producing tree legumes	245:295	the highly efficient biomass producing tree legumes	245:295	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	4	55	from	50°C	595:598	arg1	incubation					581:590	45min incubation	575:590	45min incubation at 50°C	575:598	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	5	56	theme	maximum	934:940	arg1	release					950:956	the maximum glucose release	930:956	the maximum glucose release	930:956	Of the biomass of different particle sizes (40-200mesh) used for saccharification, 40-60mesh shown the maximum glucose release.
26890795	2	57	theme	Senna	224:228	arg1	one					238:240	one	238:240	one	238:240	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	2	57	theme	Senna	224:228	arg1	siamea					230:235	Senna siamea	224:235	Senna siamea	224:235	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	2	57	theme	Senna	224:228	arg1	legumes					289:295	the highly efficient biomass producing tree legumes	245:295	the highly efficient biomass producing tree legumes	245:295	Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes.
26890795	1	58	theme	biomass	115:121	arg1	legume					138:143	An efficient biomass producing tree legume	102:143	An efficient biomass producing tree legume	102:143	H.S. Irwin & Barneby: An efficient biomass producing tree legume.
26890795	4	59	theme	phosphoric	558:567	arg1	acid					569:572	85% phosphoric acid	554:572	85% phosphoric acid	554:572	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	0	60	theme	siamea	66:71	arg1	saccharification					40:55	saccharification	40:55	saccharification of Senna siamea	40:71	Chemical composition, pretreatments and saccharification of Senna siamea (Lam.)
26890795	0	60	theme	siamea	66:71	arg1	pretreatments					22:34	pretreatments	22:34	pretreatments	22:34	Chemical composition, pretreatments and saccharification of Senna siamea (Lam.)
26890795	0	60	theme	siamea	66:71	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, pretreatments and saccharification of Senna siamea (Lam.)
26890795	4	61	theme	lignocellulose	467:480	arg1	fractionation					482:494	organic solvent-based lignocellulose fractionation	445:494	organic solvent-based lignocellulose fractionation	445:494	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
26890795	1	62	theme	producing	123:131	arg1	legume					138:143	An efficient biomass producing tree legume	102:143	An efficient biomass producing tree legume	102:143	H.S. Irwin & Barneby: An efficient biomass producing tree legume.
26890795	4	63	theme	%	556:556	arg1	acid					569:572	85% phosphoric acid	554:572	85% phosphoric acid	554:572	For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan.
29037708	0	0	theme	grass	71:75	arg1	carp					77:80	raw grass carp	67:80	raw grass carp (Ctenopharyngodon idellus)	67:107	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	0	0	theme	grass	71:75	arg1	idellus					100:106	Ctenopharyngodon idellus	83:106	Ctenopharyngodon idellus	83:106	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	4	1	theme	oil	837:839	arg1	treatments					785:794	The coating treatments	773:794	The coating treatments	773:794	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	4	1	theme	oil	837:839	arg1	coatings					851:858	chitosan-clove bud essential oil composite coatings	808:858	especially chitosan-clove bud essential oil composite coatings	797:858	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	5	2	theme	glycerol	1076:1083	arg1	monolaurate					1085:1095	glycerol monolaurate	1076:1095	glycerol monolaurate	1076:1095	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	1	3	theme	carp	251:254	arg1	fillets					256:262	refrigerated grass carp fillets	232:262	refrigerated grass carp fillets	232:262	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	0	4	theme	raw	67:69	arg1	carp					77:80	raw grass carp	67:80	raw grass carp (Ctenopharyngodon idellus)	67:107	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	0	4	theme	raw	67:69	arg1	idellus					100:106	Ctenopharyngodon idellus	83:106	Ctenopharyngodon idellus	83:106	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	0	5	from	effects	4:10	arg1	quality					56:62	flavor quality	49:62	flavor quality of raw grass carp (Ctenopharyngodon idellus)	49:107	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	4	6	theme	essential	827:835	arg1	treatments					785:794	The coating treatments	773:794	The coating treatments	773:794	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	4	6	theme	essential	827:835	arg1	coatings					851:858	chitosan-clove bud essential oil composite coatings	808:858	especially chitosan-clove bud essential oil composite coatings	797:858	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	1	7	theme	electronic	391:400	arg1	analysis					407:414	electronic nose analysis	391:414	electronic nose analysis	391:414	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	1	8	theme	fillets	256:262	arg1	retention					219:227	flavor retention	212:227	flavor retention of refrigerated grass carp fillets	212:262	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	1	9	theme	nose	402:405	arg1	analysis					407:414	electronic nose analysis	391:414	electronic nose analysis	391:414	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	5	10	theme	refrigerated	1232:1243	arg1	storage					1245:1251	refrigerated storage	1232:1251	refrigerated storage	1232:1251	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	0	11	theme	carp	77:80	arg1	quality					56:62	flavor quality	49:62	flavor quality of raw grass carp (Ctenopharyngodon idellus)	49:107	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	3	12	theme	volatile	679:686	arg1	aldehydes					728:736	many C5-C9 aldehydes	717:736	many C5-C9 aldehydes	717:736	GC-MS analysis showed 23 volatile organic compounds, including many C5-C9 aldehydes and alcohols in the fresh fillets.
29037708	3	12	theme	volatile	679:686	arg1	compounds					696:704	23 volatile organic compounds	676:704	23 volatile organic compounds	676:704	GC-MS analysis showed 23 volatile organic compounds, including many C5-C9 aldehydes and alcohols in the fresh fillets.
29037708	3	12	theme	volatile	679:686	arg1	alcohols					742:749	alcohols	742:749	alcohols	742:749	GC-MS analysis showed 23 volatile organic compounds, including many C5-C9 aldehydes and alcohols in the fresh fillets.
29037708	4	13	theme	composite	841:849	arg1	treatments					785:794	The coating treatments	773:794	The coating treatments	773:794	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	4	13	theme	composite	841:849	arg1	coatings					851:858	chitosan-clove bud essential oil composite coatings	808:858	especially chitosan-clove bud essential oil composite coatings	797:858	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	2	14	theme	inosine	600:606	arg1	monophosphate					608:620	inosine monophosphate	600:620	inosine monophosphate (IMP)	600:626	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	2	14	theme	inosine	600:606	arg1	IMP					623:625	IMP	623:625	IMP	623:625	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	5	15	theme	grass	1176:1180	arg1	fillets					1187:1193	grass carp fillets	1176:1193	grass carp fillets	1176:1193	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	2	16	theme	significant	487:497	arg1	reduction					499:507	the significant reduction	483:507	the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine,	483:578	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	3	17	theme	organic	688:694	arg1	aldehydes					728:736	many C5-C9 aldehydes	717:736	many C5-C9 aldehydes	717:736	GC-MS analysis showed 23 volatile organic compounds, including many C5-C9 aldehydes and alcohols in the fresh fillets.
29037708	3	17	theme	organic	688:694	arg1	compounds					696:704	23 volatile organic compounds	676:704	23 volatile organic compounds	676:704	GC-MS analysis showed 23 volatile organic compounds, including many C5-C9 aldehydes and alcohols in the fresh fillets.
29037708	3	17	theme	organic	688:694	arg1	alcohols					742:749	alcohols	742:749	alcohols	742:749	GC-MS analysis showed 23 volatile organic compounds, including many C5-C9 aldehydes and alcohols in the fresh fillets.
29037708	0	18	theme	Ctenopharyngodon	83:98	arg1	carp					77:80	raw grass carp	67:80	raw grass carp (Ctenopharyngodon idellus)	67:107	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	0	18	theme	Ctenopharyngodon	83:98	arg1	idellus					100:106	Ctenopharyngodon idellus	83:106	Ctenopharyngodon idellus	83:106	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	2	19	theme	umami-associated	632:647	arg1	FAA					649:651	umami-associated FAA	632:651	umami-associated FAA	632:651	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	4	20	theme	off-odor	901:908	arg1	volatiles					910:918	off-odor volatiles	901:918	off-odor volatiles	901:918	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	4	20	theme	off-odor	901:908	arg1	octanal					938:944	octanal	938:944	octanal	938:944	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	4	20	theme	off-odor	901:908	arg1	1-octen-3-ol					950:961	1-octen-3-ol	950:961	1-octen-3-ol	950:961	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	4	20	theme	off-odor	901:908	arg1	hexanal					929:935	hexanal	929:935	hexanal	929:935	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	2	21	theme	chitosan-based	444:457	arg1	coatings					459:466	chitosan-based coatings	444:466	chitosan-based coatings	444:466	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	4	22	theme	bud	823:825	arg1	treatments					785:794	The coating treatments	773:794	The coating treatments	773:794	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	4	22	theme	bud	823:825	arg1	coatings					851:858	chitosan-clove bud essential oil composite coatings	808:858	especially chitosan-clove bud essential oil composite coatings	797:858	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	5	23	theme	promising	1131:1139	arg1	method					1141:1146	a promising method	1129:1146	a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage	1129:1251	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	5	23	theme	promising	1131:1139	arg1	coating					1054:1060	chitosan coating	1045:1060	chitosan coating combined with glycerol monolaurate and clove bud essential oil	1045:1123	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	2	24	theme	compounds	523:531	arg1	reduction					499:507	the significant reduction	483:507	the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine,	483:578	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	2	24	theme	compounds	523:531	arg1	accumulation					584:595	accumulation	584:595	accumulation of inosine monophosphate (IMP) and umami-associated FAA	584:651	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	1	25	theme	various	273:279	arg1	indicators					281:290	various indicators	273:290	various indicators	273:290	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	4	26	theme	chitosan-clove	808:821	arg1	treatments					785:794	The coating treatments	773:794	The coating treatments	773:794	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	4	26	theme	chitosan-clove	808:821	arg1	coatings					851:858	chitosan-clove bud essential oil composite coatings	808:858	especially chitosan-clove bud essential oil composite coatings	797:858	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	2	27	theme	monophosphate	608:620	arg1	reduction					499:507	the significant reduction	483:507	the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine,	483:578	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	2	27	theme	monophosphate	608:620	arg1	accumulation					584:595	accumulation	584:595	accumulation of inosine monophosphate (IMP) and umami-associated FAA	584:651	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	0	28	theme	edible	15:20	arg1	coatings					37:44	edible chitosan-based coatings	15:44	edible chitosan-based coatings	15:44	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	3	29	theme	many	717:720	arg1	aldehydes					728:736	many C5-C9 aldehydes	717:736	many C5-C9 aldehydes	717:736	GC-MS analysis showed 23 volatile organic compounds, including many C5-C9 aldehydes and alcohols in the fresh fillets.
29037708	5	30	theme	sensory	996:1002	arg1	evaluation					1004:1013	the sensory evaluation	992:1013	the sensory evaluation	992:1013	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	1	31	theme	chitosan-based	185:198	arg1	coatings					200:207	chitosan-based coatings	185:207	chitosan-based coatings	185:207	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	5	32	dep	monolaurate	1085:1095	arg1	oil					1121:1123	essential oil	1111:1123	essential oil	1111:1123	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	4	33	theme	relative	881:888	arg1	content					890:896	the relative content	877:896	the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol	877:961	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	5	34	theme	nose	1030:1033	arg1	analyses					1035:1042	electronic nose analyses	1019:1042	electronic nose analyses	1019:1042	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	1	35	theme	coatings	200:207	arg1	effects					174:180	the effects	170:180	the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets	170:262	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	0	36	theme	coatings	37:44	arg1	effects					4:10	The effects	0:10	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus)	0:107	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	1	37	theme	free	293:296	arg1	acids					304:308	free amino acids	293:308	free amino acids (FAA)	293:314	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	1	37	theme	free	293:296	arg1	FAA					311:313	FAA	311:313	FAA	311:313	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	0	38	theme	refrigerated	124:135	arg1	storage					137:143	refrigerated storage	124:143	refrigerated storage	124:143	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	1	39	theme	volatile	352:359	arg1	profile					361:367	volatile profile	352:367	volatile profile	352:367	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	0	40	theme	chitosan-based	22:35	arg1	coatings					37:44	edible chitosan-based coatings	15:44	edible chitosan-based coatings	15:44	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	1	41	theme	amino	298:302	arg1	acids					304:308	free amino acids	293:308	free amino acids (FAA)	293:314	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	1	41	theme	amino	298:302	arg1	FAA					311:313	FAA	311:313	FAA	311:313	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	2	42	theme	off-flavor	512:521	arg1	TMA					542:544	TMA	542:544	TMA	542:544	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	2	42	theme	off-flavor	512:521	arg1	compounds					523:531	off-flavor compounds	512:531	off-flavor compounds	512:531	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	2	42	theme	off-flavor	512:521	arg1	histidine					569:577	histidine	569:577	histidine	569:577	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	2	42	theme	off-flavor	512:521	arg1	hypoxanthine					547:558	hypoxanthine	547:558	hypoxanthine (Hx)	547:563	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	1	43	theme	flavor	212:217	arg1	retention					219:227	flavor retention	212:227	flavor retention of refrigerated grass carp fillets	212:262	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	0	44	theme	flavor	49:54	arg1	quality					56:62	flavor quality	49:62	flavor quality of raw grass carp (Ctenopharyngodon idellus)	49:107	The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage.
29037708	5	45	theme	essential	1111:1119	arg1	oil					1121:1123	essential oil	1111:1123	essential oil	1111:1123	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	5	46	theme	chitosan	1045:1052	arg1	method					1141:1146	a promising method	1129:1146	a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage	1129:1251	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	5	46	theme	chitosan	1045:1052	arg1	coating					1054:1060	chitosan coating	1045:1060	chitosan coating combined with glycerol monolaurate and clove bud essential oil	1045:1123	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	5	47	theme	clove	1101:1105	arg1	bud					1107:1109	clove bud	1101:1109	clove bud	1101:1109	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	3	48	theme	C5-C9	722:726	arg1	aldehydes					728:736	many C5-C9 aldehydes	717:736	many C5-C9 aldehydes	717:736	GC-MS analysis showed 23 volatile organic compounds, including many C5-C9 aldehydes and alcohols in the fresh fillets.
29037708	1	49	theme	sensory	370:376	arg1	quality					378:384	sensory quality	370:384	sensory quality	370:384	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	4	50	theme	coating	777:783	arg1	coatings					851:858	chitosan-clove bud essential oil composite coatings	808:858	especially chitosan-clove bud essential oil composite coatings	797:858	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	4	50	theme	coating	777:783	arg1	treatments					785:794	The coating treatments	773:794	The coating treatments	773:794	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	5	51	theme	evaluation	1004:1013	arg1	results					981:987	the results	977:987	the results of the sensory evaluation	977:1013	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	5	51	theme	evaluation	1004:1013	arg1	analyses					1035:1042	electronic nose analyses	1019:1042	electronic nose analyses	1019:1042	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	2	52	theme	FAA	649:651	arg1	reduction					499:507	the significant reduction	483:507	the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine,	483:578	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	2	52	theme	FAA	649:651	arg1	accumulation					584:595	accumulation	584:595	accumulation of inosine monophosphate (IMP) and umami-associated FAA	584:651	The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA.
29037708	3	53	theme	fresh	758:762	arg1	fillets					764:770	the fresh fillets	754:770	the fresh fillets	754:770	GC-MS analysis showed 23 volatile organic compounds, including many C5-C9 aldehydes and alcohols in the fresh fillets.
29037708	1	54	from	effects	174:180	arg1	retention					219:227	flavor retention	212:227	flavor retention of refrigerated grass carp fillets	212:262	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	4	55	theme	volatiles	910:918	arg1	content					890:896	the relative content	877:896	the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol	877:961	The coating treatments, especially chitosan-clove bud essential oil composite coatings, sharply reduced the relative content of off-odor volatiles, such as hexanal, octanal and 1-octen-3-ol.
29037708	5	56	theme	fillets	1187:1193	arg1	edibility					1163:1171	the edibility	1159:1171	the edibility of grass carp fillets	1159:1193	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	5	57	theme	electronic	1019:1028	arg1	analyses					1035:1042	electronic nose analyses	1019:1042	electronic nose analyses	1019:1042	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	1	58	theme	refrigerated	232:243	arg1	fillets					256:262	refrigerated grass carp fillets	232:262	refrigerated grass carp fillets	232:262	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
29037708	3	59	theme	GC-MS	654:658	arg1	analysis					660:667	GC-MS analysis	654:667	GC-MS analysis	654:667	GC-MS analysis showed 23 volatile organic compounds, including many C5-C9 aldehydes and alcohols in the fresh fillets.
29037708	5	60	theme	carp	1182:1185	arg1	fillets					1187:1193	grass carp fillets	1176:1193	grass carp fillets	1176:1193	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	5	61	theme	flavor	1210:1215	arg1	quality					1217:1223	flavor quality	1210:1223	flavor quality	1210:1223	According to the results of the sensory evaluation and electronic nose analyses, chitosan coating combined with glycerol monolaurate and clove bud essential oil was a promising method to improve the edibility of grass carp fillets by maintaining flavor quality during refrigerated storage.
29037708	1	62	theme	grass	245:249	arg1	fillets					256:262	refrigerated grass carp fillets	232:262	refrigerated grass carp fillets	232:262	This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis.
26342146	4	0	from	energies	631:638	arg1	solution					668:675	aqueous solution	660:675	aqueous solution	660:675	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	6	1	theme	water	1184:1188	arg1	turbidity					1157:1165	turbidity	1157:1165	turbidity	1157:1165	These results demonstrate that enhanced coagulation achieved by plasma modification represents a viable yet advanced technology for wastewater management, and is capable of synthesizing new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated.
26342146	6	1	theme	water	1184:1188	arg1	color					1171:1175	color	1171:1175	color	1171:1175	These results demonstrate that enhanced coagulation achieved by plasma modification represents a viable yet advanced technology for wastewater management, and is capable of synthesizing new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated.
26342146	0	2	theme	magnetic	70:77	arg1	bentonite					79:87	magnetic bentonite	70:87	magnetic bentonite	70:87	The highly effective removal of Cs⁺ by low turbidity chitosan-grafted magnetic bentonite.
26342146	6	3	theme	advanced	990:997	arg1	technology					999:1008	a viable yet advanced technology	977:1008	a viable yet advanced technology for wastewater management	977:1034	These results demonstrate that enhanced coagulation achieved by plasma modification represents a viable yet advanced technology for wastewater management, and is capable of synthesizing new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated.
26342146	5	4	from	stability	682:690	arg1	groundwater					730:740	simulated groundwater	720:740	simulated groundwater	720:740	The stability of the CS-g-MB composite in simulated groundwater and in actual seawater was also investigated and this material was found to be a good candidate for the remediation of both types of water.
26342146	5	4	from	stability	682:690	arg1	seawater					756:763	actual seawater	749:763	actual seawater	749:763	The stability of the CS-g-MB composite in simulated groundwater and in actual seawater was also investigated and this material was found to be a good candidate for the remediation of both types of water.
26342146	4	5	theme	Li	475:476	arg1	ions					492:495	Mg(2+), K(+), Li(+), and Na(+) ions	461:495	Mg(2+), K(+), Li(+), and Na(+) ions	461:495	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	6	theme	Mg	461:462	arg1	ions					492:495	Mg(2+), K(+), Li(+), and Na(+) ions	461:495	Mg(2+), K(+), Li(+), and Na(+) ions	461:495	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	7	theme	hydrated	602:609	arg1	radii					611:615	the hydrated radii	598:615	the hydrated radii	598:615	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	8	from	solution	668:675	arg1	exchange					508:515	the Cs(+) exchange	498:515	the Cs(+) exchange	498:515	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	8	from	solution	668:675	arg1	result					588:593	a result	586:593	a result of the hydrated radii and hydration energies of these cations in aqueous solution	586:675	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	9	theme	aqueous	660:666	arg1	solution					668:675	aqueous solution	660:675	aqueous solution	660:675	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	10	theme	≈Mg	553:555	arg1	<Na					560:562	≈Mg(2+)<Na	553:562	≈Mg(2+)<Na	553:562	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	6	11	theme	enhanced	913:920	arg1	coagulation					922:932	enhanced coagulation	913:932	enhanced coagulation achieved by plasma modification	913:964	These results demonstrate that enhanced coagulation achieved by plasma modification represents a viable yet advanced technology for wastewater management, and is capable of synthesizing new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated.
26342146	2	12	theme	aqueous	285:291	arg1	solution					293:300	aqueous solution	285:300	aqueous solution	285:300	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	2	13	from	properties	236:245	arg1	solution					293:300	aqueous solution	285:300	aqueous solution	285:300	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	6	14	theme	wastewater	1014:1023	arg1	management					1025:1034	wastewater management	1014:1034	wastewater management	1014:1034	These results demonstrate that enhanced coagulation achieved by plasma modification represents a viable yet advanced technology for wastewater management, and is capable of synthesizing new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated.
26342146	1	15	theme	plasma-induced	171:184	arg1	method					186:191	a plasma-induced method	169:191	a plasma-induced method	169:191	Chitosan-grafted magnetic bentonite (CS-g-MB) was successfully synthesized via a plasma-induced method.
26342146	6	16	theme	viable	979:984	arg1	technology					999:1008	a viable yet advanced technology	977:1008	a viable yet advanced technology for wastewater management	977:1034	These results demonstrate that enhanced coagulation achieved by plasma modification represents a viable yet advanced technology for wastewater management, and is capable of synthesizing new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated.
26342146	4	17	theme	2+	557:558	arg1	<Na					560:562	≈Mg(2+)<Na	553:562	≈Mg(2+)<Na	553:562	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	6	18	theme	increasing	1142:1151	arg1	turbidity					1157:1165	turbidity	1157:1165	turbidity	1157:1165	These results demonstrate that enhanced coagulation achieved by plasma modification represents a viable yet advanced technology for wastewater management, and is capable of synthesizing new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated.
26342146	5	19	theme	types	866:870	arg1	remediation					846:856	the remediation	842:856	the remediation of both types of water	842:879	The stability of the CS-g-MB composite in simulated groundwater and in actual seawater was also investigated and this material was found to be a good candidate for the remediation of both types of water.
26342146	5	20	theme	good	823:826	arg1	material					796:803	this material	791:803	this material	791:803	The stability of the CS-g-MB composite in simulated groundwater and in actual seawater was also investigated and this material was found to be a good candidate for the remediation of both types of water.
26342146	5	20	theme	good	823:826	arg1	candidate					828:836	a good candidate	821:836	a good candidate for the remediation of both types of water	821:879	The stability of the CS-g-MB composite in simulated groundwater and in actual seawater was also investigated and this material was found to be a good candidate for the remediation of both types of water.
26342146	2	21	theme	adsorption	327:336	arg1	capacity					338:345	significant adsorption capacity	315:345	significant adsorption capacity for Cs(+) ions	315:360	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	3	22	theme	Cs	381:382	arg1	dependent					401:409	dependent	401:409	dependent	401:409	The adsorption of Cs(+) by CS-g-MB is dependent on both pH and ionic strength.
26342146	3	22	theme	Cs	381:382	arg1	adsorption					367:376	The adsorption	363:376	The adsorption of Cs(+) by CS-g-MB	363:396	The adsorption of Cs(+) by CS-g-MB is dependent on both pH and ionic strength.
26342146	5	23	theme	water	875:879	arg1	types					866:870	both types	861:870	both types of water	861:879	The stability of the CS-g-MB composite in simulated groundwater and in actual seawater was also investigated and this material was found to be a good candidate for the remediation of both types of water.
26342146	4	24	from	radii	611:615	arg1	solution					668:675	aqueous solution	660:675	aqueous solution	660:675	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	2	25	theme	significant	315:325	arg1	capacity					338:345	significant adsorption capacity	315:345	significant adsorption capacity for Cs(+) ions	315:360	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	4	26	theme	Li	548:549	arg1	<K					566:567	Li(+)≈Mg(2+)<Na(+)<K	548:567	Li(+)≈Mg(2+)<Na(+)<K	548:567	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	6	27	theme	new	1068:1070	arg1	adsorbents					1072:1081	new adsorbents	1068:1081	new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated	1068:1202	These results demonstrate that enhanced coagulation achieved by plasma modification represents a viable yet advanced technology for wastewater management, and is capable of synthesizing new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated.
26342146	1	28	theme	magnetic	107:114	arg1	CS-g-MB					127:133	CS-g-MB	127:133	CS-g-MB	127:133	Chitosan-grafted magnetic bentonite (CS-g-MB) was successfully synthesized via a plasma-induced method.
26342146	1	28	theme	magnetic	107:114	arg1	bentonite					116:124	Chitosan-grafted magnetic bentonite	90:124	Chitosan-grafted magnetic bentonite (CS-g-MB)	90:134	Chitosan-grafted magnetic bentonite (CS-g-MB) was successfully synthesized via a plasma-induced method.
26342146	2	29	from	turbidity	252:260	arg1	solution					293:300	aqueous solution	285:300	aqueous solution	285:300	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	1	30	theme	Chitosan-grafted	90:105	arg1	CS-g-MB					127:133	CS-g-MB	127:133	CS-g-MB	127:133	Chitosan-grafted magnetic bentonite (CS-g-MB) was successfully synthesized via a plasma-induced method.
26342146	1	30	theme	Chitosan-grafted	90:105	arg1	bentonite					116:124	Chitosan-grafted magnetic bentonite	90:124	Chitosan-grafted magnetic bentonite (CS-g-MB)	90:134	Chitosan-grafted magnetic bentonite (CS-g-MB) was successfully synthesized via a plasma-induced method.
26342146	3	31	theme	ionic	426:430	arg1	strength					432:439	ionic strength	426:439	ionic strength	426:439	The adsorption of Cs(+) by CS-g-MB is dependent on both pH and ionic strength.
26342146	0	32	theme	Cs⁺	32:34	arg1	removal					21:27	The highly effective removal	0:27	The highly effective removal of Cs⁺ by low turbidity	0:51	The highly effective removal of Cs⁺ by low turbidity chitosan-grafted magnetic bentonite.
26342146	2	33	theme	low	248:250	arg1	turbidity					252:260	low turbidity	248:260	low turbidity	248:260	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	4	34	theme	Cs	502:503	arg1	exchange					508:515	the Cs(+) exchange	498:515	the Cs(+) exchange	498:515	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	34	theme	Cs	502:503	arg1	result					588:593	a result	586:593	a result of the hydrated radii and hydration energies of these cations in aqueous solution	586:675	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	6	35	dep	avoid	1136:1140	arg1	treated					1196:1202	treated	1196:1202	being treated	1190:1202	These results demonstrate that enhanced coagulation achieved by plasma modification represents a viable yet advanced technology for wastewater management, and is capable of synthesizing new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated.
26342146	4	36	theme	cations	649:655	arg1	radii					611:615	the hydrated radii	598:615	the hydrated radii	598:615	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	36	theme	cations	649:655	arg1	energies					631:638	hydration energies	621:638	hydration energies	621:638	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	37	theme	<K	566:567	arg1	order					539:543	the order	535:543	the order of Li(+)≈Mg(2+)<Na(+)<K(+)	535:570	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	38	theme	ions	492:495	arg1	presence					449:456	the presence	445:456	the presence of Mg(2+), K(+), Li(+), and Na(+) ions	445:495	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	0	39	theme	low	39:41	arg1	turbidity					43:51	low turbidity	39:51	low turbidity	39:51	The highly effective removal of Cs⁺ by low turbidity chitosan-grafted magnetic bentonite.
26342146	6	40	dep	turbidity	1157:1165	arg1	the					1153:1155	the	1153:1155	the	1153:1155	These results demonstrate that enhanced coagulation achieved by plasma modification represents a viable yet advanced technology for wastewater management, and is capable of synthesizing new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated.
26342146	5	41	theme	actual	749:754	arg1	seawater					756:763	actual seawater	749:763	actual seawater	749:763	The stability of the CS-g-MB composite in simulated groundwater and in actual seawater was also investigated and this material was found to be a good candidate for the remediation of both types of water.
26342146	4	42	from	result	588:593	arg1	solution					668:675	aqueous solution	660:675	aqueous solution	660:675	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	43	theme	K	469:469	arg1	ions					492:495	Mg(2+), K(+), Li(+), and Na(+) ions	461:495	Mg(2+), K(+), Li(+), and Na(+) ions	461:495	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	44	theme	radii	611:615	arg1	exchange					508:515	the Cs(+) exchange	498:515	the Cs(+) exchange	498:515	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	44	theme	radii	611:615	arg1	result					588:593	a result	586:593	a result of the hydrated radii and hydration energies of these cations in aqueous solution	586:675	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	2	45	theme	good	222:225	arg1	properties					236:245	good magnetic properties	222:245	good magnetic properties	222:245	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	2	46	theme	magnetic	227:234	arg1	properties					236:245	good magnetic properties	222:245	good magnetic properties	222:245	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	2	47	theme	Cs	351:352	arg1	ions					357:360	Cs(+) ions	351:360	Cs(+) ions	351:360	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	4	48	theme	energies	631:638	arg1	exchange					508:515	the Cs(+) exchange	498:515	the Cs(+) exchange	498:515	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	48	theme	energies	631:638	arg1	result					588:593	a result	586:593	a result of the hydrated radii and hydration energies of these cations in aqueous solution	586:675	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	5	49	theme	composite	707:715	arg1	stability					682:690	The stability	678:690	The stability of the CS-g-MB composite in simulated groundwater and in actual seawater	678:763	The stability of the CS-g-MB composite in simulated groundwater and in actual seawater was also investigated and this material was found to be a good candidate for the remediation of both types of water.
26342146	2	50	theme	high	267:270	arg1	stability					272:280	high stability	267:280	high stability	267:280	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	6	51	theme	plasma	946:951	arg1	modification					953:964	plasma modification	946:964	plasma modification	946:964	These results demonstrate that enhanced coagulation achieved by plasma modification represents a viable yet advanced technology for wastewater management, and is capable of synthesizing new adsorbents that can be readily separated from solution and that avoid increasing the turbidity and color of the water being treated.
26342146	2	52	from	stability	272:280	arg1	solution					293:300	aqueous solution	285:300	aqueous solution	285:300	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	4	53	theme	hydration	621:629	arg1	energies					631:638	hydration energies	621:638	hydration energies	621:638	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	4	54	theme	<Na	560:562	arg1	<K					566:567	Li(+)≈Mg(2+)<Na(+)<K	548:567	Li(+)≈Mg(2+)<Na(+)<K	548:567	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
26342146	2	55	theme	CS-g-MB	198:204	arg1	composite					206:214	The CS-g-MB composite	194:214	The CS-g-MB composite	194:214	The CS-g-MB composite shows good magnetic properties, low turbidity, and high stability in aqueous solution and exhibits significant adsorption capacity for Cs(+) ions.
26342146	5	56	theme	simulated	720:728	arg1	groundwater					730:740	simulated groundwater	720:740	simulated groundwater	720:740	The stability of the CS-g-MB composite in simulated groundwater and in actual seawater was also investigated and this material was found to be a good candidate for the remediation of both types of water.
26342146	0	57	theme	effective	11:19	arg1	removal					21:27	The highly effective removal	0:27	The highly effective removal of Cs⁺ by low turbidity	0:51	The highly effective removal of Cs⁺ by low turbidity chitosan-grafted magnetic bentonite.
26342146	5	58	theme	CS-g-MB	699:705	arg1	composite					707:715	the CS-g-MB composite	695:715	the CS-g-MB composite	695:715	The stability of the CS-g-MB composite in simulated groundwater and in actual seawater was also investigated and this material was found to be a good candidate for the remediation of both types of water.
26342146	4	59	theme	Na	486:487	arg1	ions					492:495	Mg(2+), K(+), Li(+), and Na(+) ions	461:495	Mg(2+), K(+), Li(+), and Na(+) ions	461:495	In the presence of Mg(2+), K(+), Li(+), and Na(+) ions, the Cs(+) exchange is constrained in the order of Li(+)≈Mg(2+)<Na(+)<K(+), primarily as a result of the hydrated radii and hydration energies of these cations in aqueous solution.
24831253	6	0	theme	acid	1193:1196	arg1	activators					1198:1207	carboxylic acid activators	1182:1207	carboxylic acid activators in ethanol	1182:1218	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	2	1	theme	Matrix-assisted	274:288	arg1	MS					365:366	MS	365:366	MS	365:366	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	2	1	theme	Matrix-assisted	274:288	arg1	spectrometry					351:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry	274:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS)	274:367	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	5	2	theme	rapid	928:932	arg1	method					979:984	a rapid, robust and linkage-specific high-throughput method	926:984	a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis	926:1040	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	7	3	theme	matrix	1562:1567	arg1	substance					1569:1577	the matrix substance	1558:1577	the matrix substance	1558:1577	Glycans were recovered by hydrophilic interaction liquid chromatography solid phase extraction and analyzed by MALDI-TOF-MS in reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance.
24831253	5	4	theme	robust	935:940	arg1	method					979:984	a rapid, robust and linkage-specific high-throughput method	926:984	a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis	926:1040	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	1	5	theme	Protein	124:130	arg1	modification					181:192	an important post-translational modification	149:192	an important post-translational modification associated, among others, with diseases and the efficacy of biopharmaceuticals	149:271	Protein glycosylation is an important post-translational modification associated, among others, with diseases and the efficacy of biopharmaceuticals.
24831253	1	5	theme	Protein	124:130	arg1	glycosylation					132:144	Protein glycosylation	124:144	Protein glycosylation	124:144	Protein glycosylation is an important post-translational modification associated, among others, with diseases and the efficacy of biopharmaceuticals.
24831253	1	6	theme	important	152:160	arg1	modification					181:192	an important post-translational modification	149:192	an important post-translational modification associated, among others, with diseases and the efficacy of biopharmaceuticals	149:271	Protein glycosylation is an important post-translational modification associated, among others, with diseases and the efficacy of biopharmaceuticals.
24831253	1	6	theme	important	152:160	arg1	glycosylation					132:144	Protein glycosylation	124:144	Protein glycosylation	124:144	Protein glycosylation is an important post-translational modification associated, among others, with diseases and the efficacy of biopharmaceuticals.
24831253	8	7	theme	human	1596:1600	arg1	N-glycome					1609:1617	the human plasma N-glycome	1592:1617	the human plasma N-glycome	1592:1617	Analysis of the human plasma N-glycome allowed high-throughput detection and relative quantitation of more than 100 distinct N-glycan compositions with varying sialic acid linkages.
24831253	6	8	theme	mild	1355:1358	arg1	conditions					1360:1369	mild conditions	1355:1369	mild conditions	1355:1369	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	2	9	theme	laser	290:294	arg1	MS					365:366	MS	365:366	MS	365:366	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	2	9	theme	laser	290:294	arg1	spectrometry					351:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry	274:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS)	274:367	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	1	10	theme	biopharmaceuticals	254:271	arg1	diseases					225:232	diseases	225:232	diseases	225:232	Protein glycosylation is an important post-translational modification associated, among others, with diseases and the efficacy of biopharmaceuticals.
24831253	1	10	theme	biopharmaceuticals	254:271	arg1	efficacy					242:249	the efficacy	238:249	the efficacy of biopharmaceuticals	238:271	Protein glycosylation is an important post-translational modification associated, among others, with diseases and the efficacy of biopharmaceuticals.
24831253	8	11	theme	N-glycome	1609:1617	arg1	Analysis					1580:1587	Analysis	1580:1587	Analysis of the human plasma N-glycome	1580:1617	Analysis of the human plasma N-glycome allowed high-throughput detection and relative quantitation of more than 100 distinct N-glycan compositions with varying sialic acid linkages.
24831253	5	12	theme	human	1135:1139	arg1	N-glycome					1148:1156	PNGase F-released human plasma N-glycome	1117:1156	PNGase F-released human plasma N-glycome	1117:1156	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	3	13	theme	carboxyl	639:646	arg1	derivatization					654:667	specific carboxyl group derivatization	630:667	specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids	630:749	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
24831253	5	14	theme	sialic	990:995	arg1	acid					997:1000	sialic acid	990:1000	sialic acid stabilization	990:1014	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	8	15	theme	high-throughput	1627:1641	arg1	detection					1643:1651	high-throughput detection	1627:1651	high-throughput detection	1627:1651	Analysis of the human plasma N-glycome allowed high-throughput detection and relative quantitation of more than 100 distinct N-glycan compositions with varying sialic acid linkages.
24831253	7	16	theme	reflectron	1499:1508	arg1	mode					1519:1522	reflectron positive mode	1499:1522	reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance	1499:1577	Glycans were recovered by hydrophilic interaction liquid chromatography solid phase extraction and analyzed by MALDI-TOF-MS in reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance.
24831253	4	17	theme	pure	804:807	arg1	samples					816:822	relatively pure glycan samples	793:822	relatively pure glycan samples	793:822	However, these methods typically require relatively pure glycan samples, show sensitivity to side reactions, and need harsh conditions or long reaction times.
24831253	1	18	theme	post-translational	162:179	arg1	modification					181:192	an important post-translational modification	149:192	an important post-translational modification associated, among others, with diseases and the efficacy of biopharmaceuticals	149:271	Protein glycosylation is an important post-translational modification associated, among others, with diseases and the efficacy of biopharmaceuticals.
24831253	1	18	theme	post-translational	162:179	arg1	glycosylation					132:144	Protein glycosylation	124:144	Protein glycosylation	124:144	Protein glycosylation is an important post-translational modification associated, among others, with diseases and the efficacy of biopharmaceuticals.
24831253	6	19	theme	variants	1325:1332	arg1	esterification					1249:1262	near-complete ethyl esterification	1229:1262	near-complete ethyl esterification of α2,6-linked sialic acids	1229:1290	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	6	19	theme	variants	1325:1332	arg1	lactonization					1296:1308	lactonization	1296:1308	lactonization of α2,3-linked variants	1296:1332	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	0	20	theme	linkage-specific	79:94	arg1	esterification					108:121	linkage-specific sialic acid esterification	79:121	linkage-specific sialic acid esterification	79:121	High-throughput profiling of protein N-glycosylation by MALDI-TOF-MS employing linkage-specific sialic acid esterification.
24831253	5	21	theme	linkage-specific	946:961	arg1	method					979:984	a rapid, robust and linkage-specific high-throughput method	926:984	a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis	926:1040	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	0	22	theme	acid	103:106	arg1	esterification					108:121	linkage-specific sialic acid esterification	79:121	linkage-specific sialic acid esterification	79:121	High-throughput profiling of protein N-glycosylation by MALDI-TOF-MS employing linkage-specific sialic acid esterification.
24831253	3	23	with	derivatization	654:667	arg1	possibility					678:688	the possibility	674:688	the possibility of discrimination between α2,3- and α2,6-linked sialic acids	674:749	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
24831253	8	24	theme	N-glycan	1705:1712	arg1	compositions					1714:1725	more than 100 distinct N-glycan compositions	1682:1725	more than 100 distinct N-glycan compositions	1682:1725	Analysis of the human plasma N-glycome allowed high-throughput detection and relative quantitation of more than 100 distinct N-glycan compositions with varying sialic acid linkages.
24831253	0	25	theme	High-throughput	0:14	arg1	profiling					16:24	High-throughput profiling	0:24	High-throughput profiling of protein N-glycosylation by MALDI-TOF-MS employing linkage-specific sialic acid esterification.	0:122	High-throughput profiling of protein N-glycosylation by MALDI-TOF-MS employing linkage-specific sialic acid esterification.
24831253	5	26	theme	MALDI-TOF-MS	1020:1031	arg1	analysis					1033:1040	MALDI-TOF-MS analysis	1020:1040	MALDI-TOF-MS analysis	1020:1040	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	3	27	theme	acids	584:588	arg1	Stabilization					535:547	Stabilization	535:547	Stabilization	535:547	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
24831253	3	27	theme	acids	584:588	arg1	neutralization					553:566	neutralization	553:566	neutralization	553:566	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
24831253	7	28	theme	phase	1450:1454	arg1	extraction					1456:1465	hydrophilic interaction liquid chromatography solid phase extraction	1398:1465	hydrophilic interaction liquid chromatography solid phase extraction	1398:1465	Glycans were recovered by hydrophilic interaction liquid chromatography solid phase extraction and analyzed by MALDI-TOF-MS in reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance.
24831253	2	29	theme	glycan	519:524	arg1	species					526:532	sialylated glycan species	508:532	sialylated glycan species	508:532	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	8	30	theme	sialic	1740:1745	arg1	linkages					1752:1759	sialic acid linkages	1740:1759	sialic acid linkages	1740:1759	Analysis of the human plasma N-glycome allowed high-throughput detection and relative quantitation of more than 100 distinct N-glycan compositions with varying sialic acid linkages.
24831253	0	31	theme	N-glycosylation	37:51	arg1	profiling					16:24	High-throughput profiling	0:24	High-throughput profiling of protein N-glycosylation by MALDI-TOF-MS employing linkage-specific sialic acid esterification.	0:122	High-throughput profiling of protein N-glycosylation by MALDI-TOF-MS employing linkage-specific sialic acid esterification.
24831253	7	32	theme	chromatography	1429:1442	arg1	extraction					1456:1465	hydrophilic interaction liquid chromatography solid phase extraction	1398:1465	hydrophilic interaction liquid chromatography solid phase extraction	1398:1465	Glycans were recovered by hydrophilic interaction liquid chromatography solid phase extraction and analyzed by MALDI-TOF-MS in reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance.
24831253	4	33	theme	reaction	895:902	arg1	times					904:908	long reaction times	890:908	long reaction times	890:908	However, these methods typically require relatively pure glycan samples, show sensitivity to side reactions, and need harsh conditions or long reaction times.
24831253	6	34	from	ethanol	1212:1218	arg1	combination					1167:1177	a combination	1165:1177	a combination of carboxylic acid activators in ethanol	1165:1218	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	5	35	theme	F-released	1124:1133	arg1	N-glycome					1148:1156	PNGase F-released human plasma N-glycome	1117:1156	PNGase F-released human plasma N-glycome	1117:1156	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	6	36	theme	near-complete	1229:1241	arg1	esterification					1249:1262	near-complete ethyl esterification	1229:1262	near-complete ethyl esterification of α2,6-linked sialic acids	1229:1290	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	3	37	link	α2,6-linked	726:736	arg1	acids					745:749	α2,3- and α2,6-linked sialic acids	716:749	α2,3- and α2,6-linked sialic acids	716:749	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
24831253	5	38	theme	impure	1075:1080	arg1	N-glycome					1148:1156	PNGase F-released human plasma N-glycome	1117:1156	PNGase F-released human plasma N-glycome	1117:1156	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	5	38	theme	impure	1075:1080	arg1	mixtures					1100:1107	impure glycan-containing mixtures	1075:1107	impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome	1075:1156	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	3	39	theme	sialic	738:743	arg1	acids					745:749	α2,3- and α2,6-linked sialic acids	716:749	α2,3- and α2,6-linked sialic acids	716:749	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
24831253	4	40	theme	harsh	870:874	arg1	conditions					876:885	harsh conditions	870:885	harsh conditions	870:885	However, these methods typically require relatively pure glycan samples, show sensitivity to side reactions, and need harsh conditions or long reaction times.
24831253	5	41	theme	mixtures	1100:1107	arg1	modification					1059:1070	direct modification	1052:1070	direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome	1052:1156	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	6	42	theme	activators	1198:1207	arg1	combination					1167:1177	a combination	1165:1177	a combination of carboxylic acid activators in ethanol	1165:1218	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	6	43	from	activators	1198:1207	arg1	ethanol					1212:1218	ethanol	1212:1218	ethanol	1212:1218	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	6	44	theme	carboxylic	1182:1191	arg1	activators					1198:1207	carboxylic acid activators	1182:1207	carboxylic acid activators in ethanol	1182:1218	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	2	45	theme	mass	346:349	arg1	MS					365:366	MS	365:366	MS	365:366	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	2	45	theme	mass	346:349	arg1	spectrometry					351:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry	274:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS)	274:367	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	6	46	theme	sialic	1279:1284	arg1	acids					1286:1290	α2,6-linked sialic acids	1267:1290	α2,6-linked sialic acids	1267:1290	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	6	47	theme	ethyl	1243:1247	arg1	esterification					1249:1262	near-complete ethyl esterification	1229:1262	near-complete ethyl esterification of α2,6-linked sialic acids	1229:1290	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	6	48	from	combination	1167:1177	arg1	ethanol					1212:1218	ethanol	1212:1218	ethanol	1212:1218	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	2	49	theme	TOF	341:343	arg1	MS					365:366	MS	365:366	MS	365:366	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	2	49	theme	TOF	341:343	arg1	spectrometry					351:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry	274:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS)	274:367	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	6	50	theme	acids	1286:1290	arg1	esterification					1249:1262	near-complete ethyl esterification	1229:1262	near-complete ethyl esterification of α2,6-linked sialic acids	1229:1290	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	6	50	theme	acids	1286:1290	arg1	lactonization					1296:1308	lactonization	1296:1308	lactonization of α2,3-linked variants	1296:1332	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	6	51	theme	α2,6-linked	1267:1277	arg1	acids					1286:1290	α2,6-linked sialic acids	1267:1290	α2,6-linked sialic acids	1267:1290	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	8	52	theme	plasma	1602:1607	arg1	N-glycome					1609:1617	the human plasma N-glycome	1592:1617	the human plasma N-glycome	1592:1617	Analysis of the human plasma N-glycome allowed high-throughput detection and relative quantitation of more than 100 distinct N-glycan compositions with varying sialic acid linkages.
24831253	7	53	theme	interaction	1410:1420	arg1	chromatography					1429:1442	hydrophilic interaction liquid chromatography	1398:1442	hydrophilic interaction liquid chromatography solid phase extraction	1398:1465	Glycans were recovered by hydrophilic interaction liquid chromatography solid phase extraction and analyzed by MALDI-TOF-MS in reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance.
24831253	2	54	theme	desorption/ionization	296:316	arg1	MS					365:366	MS	365:366	MS	365:366	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	2	54	theme	desorption/ionization	296:316	arg1	spectrometry					351:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry	274:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS)	274:367	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	3	55	theme	group	648:652	arg1	derivatization					654:667	specific carboxyl group derivatization	630:667	specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids	630:749	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
24831253	6	56	link	α2,3-linked	1313:1323	arg1	variants					1325:1332	α2,3-linked variants	1313:1332	α2,3-linked variants	1313:1332	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	3	57	theme	specific	630:637	arg1	derivatization					654:667	specific carboxyl group derivatization	630:667	specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids	630:749	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
24831253	6	58	theme	short	1338:1342	arg1	time					1344:1347	short time	1338:1347	short time using mild conditions	1338:1369	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	5	59	theme	plasma	1141:1146	arg1	N-glycome					1148:1156	PNGase F-released human plasma N-glycome	1117:1156	PNGase F-released human plasma N-glycome	1117:1156	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	2	60	theme	time-of-fight	326:338	arg1	MS					365:366	MS	365:366	MS	365:366	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	2	60	theme	time-of-fight	326:338	arg1	spectrometry					351:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry	274:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS)	274:367	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	5	61	theme	acid	997:1000	arg1	stabilization					1002:1014	sialic acid stabilization	990:1014	sialic acid stabilization	990:1014	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	8	62	theme	relative	1657:1664	arg1	quantitation					1666:1677	relative quantitation	1657:1677	relative quantitation of more than 100 distinct N-glycan compositions	1657:1725	Analysis of the human plasma N-glycome allowed high-throughput detection and relative quantitation of more than 100 distinct N-glycan compositions with varying sialic acid linkages.
24831253	7	63	theme	positive	1510:1517	arg1	mode					1519:1522	reflectron positive mode	1499:1522	reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance	1499:1577	Glycans were recovered by hydrophilic interaction liquid chromatography solid phase extraction and analyzed by MALDI-TOF-MS in reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance.
24831253	0	64	theme	sialic	96:101	arg1	esterification					108:121	linkage-specific sialic acid esterification	79:121	linkage-specific sialic acid esterification	79:121	High-throughput profiling of protein N-glycosylation by MALDI-TOF-MS employing linkage-specific sialic acid esterification.
24831253	2	65	theme	MALDI	319:323	arg1	MS					365:366	MS	365:366	MS	365:366	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	2	65	theme	MALDI	319:323	arg1	spectrometry					351:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry	274:362	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS)	274:367	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	6	66	theme	α2,3-linked	1313:1323	arg1	variants					1325:1332	α2,3-linked variants	1313:1332	α2,3-linked variants	1313:1332	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	5	67	theme	high-throughput	963:977	arg1	method					979:984	a rapid, robust and linkage-specific high-throughput method	926:984	a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis	926:1040	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	7	68	with	mode	1519:1522	arg1	acid					1550:1553	2,5-dihydroxybenzoic acid	1529:1553	2,5-dihydroxybenzoic acid	1529:1553	Glycans were recovered by hydrophilic interaction liquid chromatography solid phase extraction and analyzed by MALDI-TOF-MS in reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance.
24831253	8	69	theme	distinct	1696:1703	arg1	compositions					1714:1725	more than 100 distinct N-glycan compositions	1682:1725	more than 100 distinct N-glycan compositions	1682:1725	Analysis of the human plasma N-glycome allowed high-throughput detection and relative quantitation of more than 100 distinct N-glycan compositions with varying sialic acid linkages.
24831253	6	70	link	α2,6-linked	1267:1277	arg1	acids					1286:1290	α2,6-linked sialic acids	1267:1290	α2,6-linked sialic acids	1267:1290	Using a combination of carboxylic acid activators in ethanol achieved near-complete ethyl esterification of α2,6-linked sialic acids and lactonization of α2,3-linked variants, in short time using mild conditions.
24831253	5	71	theme	direct	1052:1057	arg1	modification					1059:1070	direct modification	1052:1070	direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome	1052:1156	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	8	72	theme	compositions	1714:1725	arg1	detection					1643:1651	high-throughput detection	1627:1651	high-throughput detection	1627:1651	Analysis of the human plasma N-glycome allowed high-throughput detection and relative quantitation of more than 100 distinct N-glycan compositions with varying sialic acid linkages.
24831253	8	72	theme	compositions	1714:1725	arg1	quantitation					1666:1677	relative quantitation	1657:1677	relative quantitation of more than 100 distinct N-glycan compositions	1657:1725	Analysis of the human plasma N-glycome allowed high-throughput detection and relative quantitation of more than 100 distinct N-glycan compositions with varying sialic acid linkages.
24831253	4	73	theme	side	845:848	arg1	reactions					850:858	side reactions	845:858	side reactions	845:858	However, these methods typically require relatively pure glycan samples, show sensitivity to side reactions, and need harsh conditions or long reaction times.
24831253	2	74	theme	ionization	477:486	arg1	bias					488:491	the instability and ionization bias	457:491	bias	488:491	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	4	75	theme	glycan	809:814	arg1	samples					816:822	relatively pure glycan samples	793:822	relatively pure glycan samples	793:822	However, these methods typically require relatively pure glycan samples, show sensitivity to side reactions, and need harsh conditions or long reaction times.
24831253	2	76	theme	high-throughput	414:428	arg1	manner					430:435	a high-throughput manner	412:435	a high-throughput manner	412:435	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	0	77	theme	protein	29:35	arg1	N-glycosylation					37:51	protein N-glycosylation	29:51	protein N-glycosylation	29:51	High-throughput profiling of protein N-glycosylation by MALDI-TOF-MS employing linkage-specific sialic acid esterification.
24831253	7	78	theme	2,5-dihydroxybenzoic	1529:1548	arg1	acid					1550:1553	2,5-dihydroxybenzoic acid	1529:1553	2,5-dihydroxybenzoic acid	1529:1553	Glycans were recovered by hydrophilic interaction liquid chromatography solid phase extraction and analyzed by MALDI-TOF-MS in reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance.
24831253	7	79	theme	solid	1444:1448	arg1	extraction					1456:1465	hydrophilic interaction liquid chromatography solid phase extraction	1398:1465	hydrophilic interaction liquid chromatography solid phase extraction	1398:1465	Glycans were recovered by hydrophilic interaction liquid chromatography solid phase extraction and analyzed by MALDI-TOF-MS in reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance.
24831253	2	80	theme	instability	461:471	arg1	bias					488:491	the instability and ionization bias	457:491	bias	488:491	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	8	81	theme	acid	1747:1750	arg1	linkages					1752:1759	sialic acid linkages	1740:1759	sialic acid linkages	1740:1759	Analysis of the human plasma N-glycome allowed high-throughput detection and relative quantitation of more than 100 distinct N-glycan compositions with varying sialic acid linkages.
24831253	3	82	theme	α2,3-	716:720	arg1	acids					745:749	α2,3- and α2,6-linked sialic acids	716:749	α2,3- and α2,6-linked sialic acids	716:749	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
24831253	2	83	gly	sialylated	508:517	arg1	species					526:532	sialylated glycan species	508:532	sialylated glycan species	508:532	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	7	84	theme	liquid	1422:1427	arg1	chromatography					1429:1442	hydrophilic interaction liquid chromatography	1398:1442	hydrophilic interaction liquid chromatography solid phase extraction	1398:1465	Glycans were recovered by hydrophilic interaction liquid chromatography solid phase extraction and analyzed by MALDI-TOF-MS in reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance.
24831253	2	85	theme	sialylated	508:517	arg1	species					526:532	sialylated glycan species	508:532	sialylated glycan species	508:532	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	2	86	gly	glycosylation	395:407	arg1	manner					430:435	a high-throughput manner	412:435	a high-throughput manner	412:435	Matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) can be performed to study glycosylation in a high-throughput manner, but is hampered by the instability and ionization bias experienced by sialylated glycan species.
24831253	3	87	theme	discrimination	693:706	arg1	possibility					678:688	the possibility	674:688	the possibility of discrimination between α2,3- and α2,6-linked sialic acids	674:749	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
24831253	5	88	theme	PNGase	1117:1122	arg1	N-glycome					1148:1156	PNGase F-released human plasma N-glycome	1117:1156	PNGase F-released human plasma N-glycome	1117:1156	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	7	89	theme	hydrophilic	1398:1408	arg1	chromatography					1429:1442	hydrophilic interaction liquid chromatography	1398:1442	hydrophilic interaction liquid chromatography solid phase extraction	1398:1465	Glycans were recovered by hydrophilic interaction liquid chromatography solid phase extraction and analyzed by MALDI-TOF-MS in reflectron positive mode with 2,5-dihydroxybenzoic acid as the matrix substance.
24831253	4	90	theme	long	890:893	arg1	times					904:908	long reaction times	890:908	long reaction times	890:908	However, these methods typically require relatively pure glycan samples, show sensitivity to side reactions, and need harsh conditions or long reaction times.
24831253	3	91	theme	α2,6-linked	726:736	arg1	acids					745:749	α2,3- and α2,6-linked sialic acids	716:749	α2,3- and α2,6-linked sialic acids	716:749	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
24831253	5	92	theme	glycan-containing	1082:1098	arg1	N-glycome					1148:1156	PNGase F-released human plasma N-glycome	1117:1156	PNGase F-released human plasma N-glycome	1117:1156	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	5	92	theme	glycan-containing	1082:1098	arg1	mixtures					1100:1107	impure glycan-containing mixtures	1075:1107	impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome	1075:1156	We established a rapid, robust and linkage-specific high-throughput method for sialic acid stabilization and MALDI-TOF-MS analysis, to allow direct modification of impure glycan-containing mixtures such as PNGase F-released human plasma N-glycome.
24831253	3	93	theme	sialic	577:582	arg1	acids					584:588	these sialic acids	571:588	these sialic acids	571:588	Stabilization and neutralization of these sialic acids can be achieved by permethylation or by specific carboxyl group derivatization with the possibility of discrimination between α2,3- and α2,6-linked sialic acids.
27872844	9	0	theme	hydroxyapatite	1624:1637	arg1	constructs					1650:1659	cellulose and hydroxyapatite reinforced constructs	1610:1659	cellulose and hydroxyapatite reinforced constructs with controlled architecture	1610:1688	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	2	1	theme	bioextrusion	365:376	arg1	system					378:383	a bioextrusion system	363:383	a bioextrusion system	363:383	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	9	2	theme	3D	1733:1734	arg1	constructs					1746:1755	these 3D composite constructs	1727:1755	these 3D composite constructs	1727:1755	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	2	3	theme	3D	405:406	arg1	scaffolds					422:430	3D biodegradable scaffolds	405:430	3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference	405:549	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	8	4	theme	cytotoxicity	1482:1493	arg1	assay					1495:1499	the cytotoxicity assay	1478:1499	the cytotoxicity assay	1478:1499	Results from the cytotoxicity assay suggest that all the composite scaffolds presented good biocompatibility.
27872844	2	5	dep	types	479:483	arg1	matrix					531:536	(i) poly(ε-caprolactone) (PCL) matrix	500:536	three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix	473:536	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	9	6	from	results	1579:1585	arg1	constructs					1650:1659	cellulose and hydroxyapatite reinforced constructs	1610:1659	cellulose and hydroxyapatite reinforced constructs with controlled architecture	1610:1688	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	4	7	theme	lay-down	994:1001	arg1	pattern					1003:1009	a single lay-down pattern	985:1009	a single lay-down pattern of 0/90°	985:1018	Scaffolds were produced using a single lay-down pattern of 0/90°, with the same processing parameters among all constructs being assured.
27872844	1	8	theme	mechanical	272:281	arg1	support					283:289	an adequate biological and mechanical support	245:289	an adequate biological and mechanical support for tissue regeneration	245:313	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	9	9	theme	mechanical	1792:1801	arg1	properties					1803:1812	enhanced mechanical properties	1783:1812	enhanced mechanical properties	1783:1812	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	8	10	theme	composite	1522:1530	arg1	scaffolds					1532:1540	all the composite scaffolds	1514:1540	all the composite scaffolds	1514:1540	Results from the cytotoxicity assay suggest that all the composite scaffolds presented good biocompatibility.
27872844	2	11	theme	PCL-based	557:565	arg1	matrix					567:572	(ii) PCL-based matrix	552:572	(ii) PCL-based matrix	552:572	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	7	12	theme	higher	1397:1402	arg1	stiffness					1413:1421	higher scaffold stiffness	1397:1421	higher scaffold stiffness in the reinforced scaffolds	1397:1449	Regarding the mechanical properties, higher scaffold stiffness in the reinforced scaffolds was obtained.
27872844	4	13	theme	single	987:992	arg1	pattern					1003:1009	a single lay-down pattern	985:1009	a single lay-down pattern of 0/90°	985:1018	Scaffolds were produced using a single lay-down pattern of 0/90°, with the same processing parameters among all constructs being assured.
27872844	2	14	with	scaffolds	422:430	arg1	architecture					448:459	controlled architecture	437:459	controlled architecture	437:459	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	5	15	theme	polymer	1183:1189	arg1	matrix					1191:1196	the polymer matrix	1179:1196	the polymer matrix	1179:1196	The performed morphological analyses showed a satisfactory distribution of CNF within the polymer matrix and high reliability was obtained among the produced scaffolds.
27872844	4	16	theme	processing	1035:1044	arg1	parameters					1046:1055	the same processing parameters	1026:1055	the same processing parameters among all constructs	1026:1076	Scaffolds were produced using a single lay-down pattern of 0/90°, with the same processing parameters among all constructs being assured.
27872844	3	17	theme	HANP	763:766	arg1	combination					740:750	combination	740:750	combination of CNF and HANP	740:766	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	5	18	theme	performed	1097:1105	arg1	analyses					1121:1128	The performed morphological analyses	1093:1128	The performed morphological analyses	1093:1128	The performed morphological analyses showed a satisfactory distribution of CNF within the polymer matrix and high reliability was obtained among the produced scaffolds.
27872844	7	19	theme	reinforced	1430:1439	arg1	scaffolds					1441:1449	the reinforced scaffolds	1426:1449	the reinforced scaffolds	1426:1449	Regarding the mechanical properties, higher scaffold stiffness in the reinforced scaffolds was obtained.
27872844	9	20	theme	controlled	1666:1675	arg1	architecture					1677:1688	controlled architecture	1666:1688	controlled architecture	1666:1688	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	1	21	theme	controlled	174:183	arg1	properties					159:168	Biomaterial properties	147:168	Biomaterial properties	147:168	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	1	21	theme	controlled	174:183	arg1	features					225:232	essential features	215:232	essential features	215:232	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	1	21	theme	controlled	174:183	arg1	architecture					185:196	controlled architecture	174:196	controlled architecture	174:196	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	8	22	from	assay	1495:1499	arg1	Results					1465:1471	Results	1465:1471	Results from the cytotoxicity assay	1465:1499	Results from the cytotoxicity assay suggest that all the composite scaffolds presented good biocompatibility.
27872844	3	23	theme	thermal	901:907	arg1	performances					925:936	morphological, thermal, and mechanical performances	886:936	morphological, thermal, and mechanical performances	886:936	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	3	23	theme	thermal	901:907	arg1	constructs					874:883	the constructs	870:883	the constructs (morphological, thermal, and mechanical performances)	870:937	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	2	24	theme	PCL-based	628:636	arg1	matrix					638:643	(iii) PCL-based matrix	622:643	(iii) PCL-based matrix	622:643	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	9	25	theme	cell	1761:1764	arg1	cultivation					1766:1776	cell cultivation	1761:1776	cell cultivation with enhanced mechanical properties	1761:1812	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	6	26	theme	thermal	1309:1315	arg1	properties					1317:1326	thermal properties	1309:1326	thermal properties	1309:1326	Significant effects on surface wettability and thermal properties were observed, among scaffolds.
27872844	7	27	from	stiffness	1413:1421	arg1	scaffolds					1441:1449	the reinforced scaffolds	1426:1449	the reinforced scaffolds	1426:1449	Regarding the mechanical properties, higher scaffold stiffness in the reinforced scaffolds was obtained.
27872844	9	28	theme	enhanced	1783:1790	arg1	properties					1803:1812	enhanced mechanical properties	1783:1812	enhanced mechanical properties	1783:1812	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	2	29	theme	cellulose	590:598	arg1	CNF					612:614	CNF	612:614	CNF	612:614	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	2	29	theme	cellulose	590:598	arg1	nanofibers					600:609	cellulose nanofibers	590:609	cellulose nanofibers (CNF)	590:615	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	0	30	theme	Cellulose	62:70	arg1	Nanofibers					72:81	Cellulose Nanofibers	62:81	Cellulose Nanofibers	62:81	Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles.
27872844	1	31	theme	tissue	295:300	arg1	regeneration					302:313	tissue regeneration	295:313	tissue regeneration	295:313	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	7	32	theme	mechanical	1374:1383	arg1	properties					1385:1394	the mechanical properties	1370:1394	the mechanical properties	1370:1394	Regarding the mechanical properties, higher scaffold stiffness in the reinforced scaffolds was obtained.
27872844	3	33	theme	addition	724:731	arg1	effect					710:715	The effect	706:715	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL	706:799	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	1	34	theme	scaffolds	201:209	arg1	features					225:232	essential features	215:232	essential features	215:232	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	1	34	theme	scaffolds	201:209	arg1	properties					159:168	Biomaterial properties	147:168	Biomaterial properties	147:168	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	1	34	theme	scaffolds	201:209	arg1	architecture					185:196	controlled architecture	174:196	controlled architecture	174:196	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	5	35	theme	morphological	1107:1119	arg1	analyses					1121:1128	The performed morphological analyses	1093:1128	The performed morphological analyses	1093:1128	The performed morphological analyses showed a satisfactory distribution of CNF within the polymer matrix and high reliability was obtained among the produced scaffolds.
27872844	8	36	theme	good	1552:1555	arg1	biocompatibility					1557:1572	good biocompatibility	1552:1572	good biocompatibility	1552:1572	Results from the cytotoxicity assay suggest that all the composite scaffolds presented good biocompatibility.
27872844	2	37	dep	matrix	638:643	arg1	iii					623:625	iii	623:625	iii	623:625	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	2	38	theme	controlled	437:446	arg1	architecture					448:459	controlled architecture	437:459	controlled architecture	437:459	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	0	39	theme	Poly	15:18	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Poly(ε-caprolactone)	0:34	Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles.
27872844	6	40	theme	surface	1285:1291	arg1	wettability					1293:1303	surface wettability	1285:1303	surface wettability	1285:1303	Significant effects on surface wettability and thermal properties were observed, among scaffolds.
27872844	4	41	theme	same	1030:1033	arg1	parameters					1046:1055	the same processing parameters	1026:1055	the same processing parameters among all constructs	1026:1076	Scaffolds were produced using a single lay-down pattern of 0/90°, with the same processing parameters among all constructs being assured.
27872844	3	42	theme	biomaterial	843:853	arg1	composition					855:865	the biomaterial composition	839:865	the biomaterial composition	839:865	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	1	43	theme	essential	215:223	arg1	properties					159:168	Biomaterial properties	147:168	Biomaterial properties	147:168	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	1	43	theme	essential	215:223	arg1	features					225:232	essential features	215:232	essential features	215:232	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	1	43	theme	essential	215:223	arg1	architecture					185:196	controlled architecture	174:196	controlled architecture	174:196	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	5	44	theme	produced	1242:1249	arg1	scaffolds					1251:1259	the produced scaffolds	1238:1259	the produced scaffolds	1238:1259	The performed morphological analyses showed a satisfactory distribution of CNF within the polymer matrix and high reliability was obtained among the produced scaffolds.
27872844	0	45	theme	Nanoparticles	132:144	arg1	Addition					105:112	the Addition	101:112	the Addition of Hydroxyapatite Nanoparticles	101:144	Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles.
27872844	5	46	theme	high	1202:1205	arg1	reliability					1207:1217	high reliability	1202:1217	high reliability	1202:1217	The performed morphological analyses showed a satisfactory distribution of CNF within the polymer matrix and high reliability was obtained among the produced scaffolds.
27872844	3	47	theme	composition	855:865	arg1	effects					828:834	the effects	824:834	the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances)	824:937	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	5	48	theme	satisfactory	1139:1150	arg1	distribution					1152:1163	a satisfactory distribution	1137:1163	a satisfactory distribution of CNF within the polymer matrix	1137:1196	The performed morphological analyses showed a satisfactory distribution of CNF within the polymer matrix and high reliability was obtained among the produced scaffolds.
27872844	2	49	theme	constructs	488:497	arg1	types					479:483	three types	473:483	three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix	473:536	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	0	50	theme	Hydroxyapatite	117:130	arg1	Nanoparticles					132:144	Hydroxyapatite Nanoparticles	117:144	Hydroxyapatite Nanoparticles	117:144	Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles.
27872844	0	51	dep	Scaffolds	36:44	arg1	Reinforced					46:55	Reinforced	46:55	Reinforced	46:55	Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles.
27872844	3	52	theme	morphological	886:898	arg1	performances					925:936	morphological, thermal, and mechanical performances	886:936	morphological, thermal, and mechanical performances	886:936	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	3	52	theme	morphological	886:898	arg1	constructs					874:883	the constructs	870:883	the constructs (morphological, thermal, and mechanical performances)	870:937	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	2	53	theme	biodegradable	408:420	arg1	scaffolds					422:430	3D biodegradable scaffolds	405:430	3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference	405:549	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	1	54	theme	ingrowth	330:337	arg1	tissues					339:345	the ingrowth tissues	326:345	the ingrowth tissues	326:345	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	6	55	theme	Significant	1262:1272	arg1	effects					1274:1280	Significant effects	1262:1280	Significant effects on surface wettability and thermal properties	1262:1326	Significant effects on surface wettability and thermal properties were observed, among scaffolds.
27872844	6	56	from	effects	1274:1280	arg1	properties					1317:1326	thermal properties	1309:1326	thermal properties	1309:1326	Significant effects on surface wettability and thermal properties were observed, among scaffolds.
27872844	6	56	from	effects	1274:1280	arg1	wettability					1293:1303	surface wettability	1285:1303	surface wettability	1285:1303	Significant effects on surface wettability and thermal properties were observed, among scaffolds.
27872844	9	57	theme	constructs	1746:1755	arg1	potential					1714:1722	the potential	1710:1722	the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties	1710:1812	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	2	58	dep	matrix	567:572	arg1	ii					553:554	ii	553:554	ii	553:554	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	9	59	theme	composite	1736:1744	arg1	constructs					1746:1755	these 3D composite constructs	1727:1755	these 3D composite constructs	1727:1755	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	9	60	theme	first	1595:1599	arg1	study					1601:1605	this first study	1590:1605	this first study	1590:1605	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	9	61	theme	reinforced	1639:1648	arg1	constructs					1650:1659	cellulose and hydroxyapatite reinforced constructs	1610:1659	cellulose and hydroxyapatite reinforced constructs with controlled architecture	1610:1688	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	9	62	with	cultivation	1766:1776	arg1	properties					1803:1812	enhanced mechanical properties	1783:1812	enhanced mechanical properties	1783:1812	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	2	63	dep	poly	504:507	arg1	i					501:501	i	501:501	i	501:501	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	9	64	theme	study	1601:1605	arg1	results					1579:1585	The results	1575:1585	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture	1575:1688	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	3	65	theme	PCL	797:799	arg1	matrix					787:792	the polymeric matrix	773:792	the polymeric matrix of PCL	773:799	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	5	66	theme	CNF	1168:1170	arg1	distribution					1152:1163	a satisfactory distribution	1137:1163	a satisfactory distribution of CNF within the polymer matrix	1137:1196	The performed morphological analyses showed a satisfactory distribution of CNF within the polymer matrix and high reliability was obtained among the produced scaffolds.
27872844	3	67	from	effects	828:834	arg1	constructs					874:883	the constructs	870:883	the constructs (morphological, thermal, and mechanical performances)	870:937	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	3	67	from	effects	828:834	arg1	performances					925:936	morphological, thermal, and mechanical performances	886:936	morphological, thermal, and mechanical performances	886:936	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	2	68	used	used	389:392	arg2	system					378:383	a bioextrusion system	363:383	a bioextrusion system	363:383	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	3	69	theme	polymeric	777:785	arg1	matrix					787:792	the polymeric matrix	773:792	the polymeric matrix of PCL	773:799	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	3	70	theme	CNF	755:757	arg1	combination					740:750	combination	740:750	combination of CNF and HANP	740:766	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	2	71	theme	poly	504:507	arg1	matrix					531:536	(i) poly(ε-caprolactone) (PCL) matrix	500:536	three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix	473:536	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	7	72	theme	scaffold	1404:1411	arg1	stiffness					1413:1421	higher scaffold stiffness	1397:1421	higher scaffold stiffness in the reinforced scaffolds	1397:1449	Regarding the mechanical properties, higher scaffold stiffness in the reinforced scaffolds was obtained.
27872844	9	73	theme	cellulose	1610:1618	arg1	constructs					1650:1659	cellulose and hydroxyapatite reinforced constructs	1610:1659	cellulose and hydroxyapatite reinforced constructs with controlled architecture	1610:1688	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	3	74	theme	mechanical	914:923	arg1	performances					925:936	morphological, thermal, and mechanical performances	886:936	morphological, thermal, and mechanical performances	886:936	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	3	74	theme	mechanical	914:923	arg1	constructs					874:883	the constructs	870:883	the constructs (morphological, thermal, and mechanical performances)	870:937	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	9	75	with	constructs	1650:1659	arg1	architecture					1677:1688	controlled architecture	1666:1688	controlled architecture	1666:1688	The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.
27872844	4	76	theme	0/90°	1014:1018	arg1	pattern					1003:1009	a single lay-down pattern	985:1009	a single lay-down pattern of 0/90°	985:1018	Scaffolds were produced using a single lay-down pattern of 0/90°, with the same processing parameters among all constructs being assured.
27872844	1	77	theme	adequate	248:255	arg1	support					283:289	an adequate biological and mechanical support	245:289	an adequate biological and mechanical support for tissue regeneration	245:313	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	3	78	theme	combination	740:750	arg1	effect					710:715	The effect	706:715	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL	706:799	The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed.
27872844	2	79	theme	hydroxyapatite	669:682	arg1	HANP					699:702	HANP	699:702	HANP	699:702	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	2	79	theme	hydroxyapatite	669:682	arg1	nanoparticles					684:696	hydroxyapatite nanoparticles	669:696	hydroxyapatite nanoparticles (HANP)	669:703	In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP).
27872844	1	80	theme	Biomaterial	147:157	arg1	features					225:232	essential features	215:232	essential features	215:232	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	1	80	theme	Biomaterial	147:157	arg1	properties					159:168	Biomaterial properties	147:168	Biomaterial properties	147:168	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	1	80	theme	Biomaterial	147:157	arg1	architecture					185:196	controlled architecture	174:196	controlled architecture	174:196	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
27872844	1	81	theme	biological	257:266	arg1	support					283:289	an adequate biological and mechanical support	245:289	an adequate biological and mechanical support for tissue regeneration	245:313	Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues.
26167700	0	0	theme	Composite	102:110	arg1	Paper					112:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Binary Synergy Strengthening and Toughening of Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper.
26167700	2	1	theme	/sodium	350:356	arg1	blocks					381:386	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	0	2	theme	Alginate	93:100	arg1	Paper					112:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Binary Synergy Strengthening and Toughening of Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper.
26167700	1	3	theme	excellent	179:187	arg1	balance					189:195	the excellent balance	175:195	the excellent balance of strength and toughness	175:221	A crucial requirement for most engineering materials is the excellent balance of strength and toughness.
26167700	1	3	theme	excellent	179:187	arg1	requirement					129:139	A crucial requirement	119:139	A crucial requirement for most engineering materials	119:170	A crucial requirement for most engineering materials is the excellent balance of strength and toughness.
26167700	5	4	theme	permeable	972:980	arg1	membranes					982:990	permeable membranes	972:990	permeable membranes	972:990	The successful fabrication route offers an excellent approach to design advanced strong integrated nacre-like composite materials, which can be applied in tissue engineering, protection, aerospace, and permeable membranes for separation and delivery.
26167700	2	5	theme	paper	302:306	arg1	kind					283:286	a kind	281:286	a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	281:386	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	3	6	theme	strength	567:574	arg1	combination					547:557	a perfect combination	537:557	a perfect combination of high strength and toughness	537:588	Systematic evaluation for the mechanical property in different (dry/wet) environment/after thermal annealing shows a perfect combination of high strength and toughness.
26167700	0	7	theme	Paper	112:116	arg1	Strengthening					15:27	Binary Synergy Strengthening	0:27	Binary Synergy Strengthening	0:27	Binary Synergy Strengthening and Toughening of Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper.
26167700	0	7	theme	Paper	112:116	arg1	Toughening					33:42	Toughening	33:42	Toughening	33:42	Binary Synergy Strengthening and Toughening of Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper.
26167700	2	8	theme	nacre-like	291:300	arg1	paper					302:306	nacre-like paper	291:306	nacre-like paper	291:306	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	2	9	theme	graphene	331:338	arg1	oxide					340:344	binary hybrid graphene oxide	317:344	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	4	10	theme	strengthening/toughening	702:725	arg1	enhancement					727:737	the synergistic strengthening/toughening enhancement	686:737	the synergistic strengthening/toughening enhancement from the binary GO/SA hybrids	686:767	Both of the parameters are nearly many-times higher than those of similar materials because of the synergistic strengthening/toughening enhancement from the binary GO/SA hybrids.
26167700	5	11	theme	advanced	842:849	arg1	materials					890:898	advanced strong integrated nacre-like composite materials	842:898	advanced strong integrated nacre-like composite materials	842:898	The successful fabrication route offers an excellent approach to design advanced strong integrated nacre-like composite materials, which can be applied in tissue engineering, protection, aerospace, and permeable membranes for separation and delivery.
26167700	5	12	theme	successful	774:783	arg1	route					797:801	The successful fabrication route	770:801	The successful fabrication route	770:801	The successful fabrication route offers an excellent approach to design advanced strong integrated nacre-like composite materials, which can be applied in tissue engineering, protection, aerospace, and permeable membranes for separation and delivery.
26167700	3	13	theme	perfect	539:545	arg1	combination					547:557	a perfect combination	537:557	a perfect combination of high strength and toughness	537:588	Systematic evaluation for the mechanical property in different (dry/wet) environment/after thermal annealing shows a perfect combination of high strength and toughness.
26167700	2	14	from	structure	261:269	arg1	nacre					274:278	nacre	274:278	nacre	274:278	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	2	15	theme	building	372:379	arg1	blocks					381:386	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	0	16	theme	Synergy	7:13	arg1	Strengthening					15:27	Binary Synergy Strengthening	0:27	Binary Synergy Strengthening	0:27	Binary Synergy Strengthening and Toughening of Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper.
26167700	5	17	theme	fabrication	785:795	arg1	route					797:801	The successful fabrication route	770:801	The successful fabrication route	770:801	The successful fabrication route offers an excellent approach to design advanced strong integrated nacre-like composite materials, which can be applied in tissue engineering, protection, aerospace, and permeable membranes for separation and delivery.
26167700	2	18	theme	hybrid	324:329	arg1	oxide					340:344	binary hybrid graphene oxide	317:344	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	2	19	dep	oxide	340:344	arg1	GO					347:348	GO	347:348	GO	347:348	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	1	20	theme	strength	200:207	arg1	balance					189:195	the excellent balance	175:195	the excellent balance of strength and toughness	175:221	A crucial requirement for most engineering materials is the excellent balance of strength and toughness.
26167700	1	20	theme	strength	200:207	arg1	requirement					129:139	A crucial requirement	119:139	A crucial requirement for most engineering materials	119:170	A crucial requirement for most engineering materials is the excellent balance of strength and toughness.
26167700	0	21	theme	Binary	0:5	arg1	Strengthening					15:27	Binary Synergy Strengthening	0:27	Binary Synergy Strengthening	0:27	Binary Synergy Strengthening and Toughening of Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper.
26167700	4	22	theme	binary	748:753	arg1	hybrids					761:767	the binary GO/SA hybrids	744:767	the binary GO/SA hybrids	744:767	Both of the parameters are nearly many-times higher than those of similar materials because of the synergistic strengthening/toughening enhancement from the binary GO/SA hybrids.
26167700	2	23	theme	binary	317:322	arg1	oxide					340:344	binary hybrid graphene oxide	317:344	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	2	24	theme	SA	368:369	arg1	blocks					381:386	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	5	25	theme	integrated	858:867	arg1	materials					890:898	advanced strong integrated nacre-like composite materials	842:898	advanced strong integrated nacre-like composite materials	842:898	The successful fabrication route offers an excellent approach to design advanced strong integrated nacre-like composite materials, which can be applied in tissue engineering, protection, aerospace, and permeable membranes for separation and delivery.
26167700	5	26	theme	strong	851:856	arg1	materials					890:898	advanced strong integrated nacre-like composite materials	842:898	advanced strong integrated nacre-like composite materials	842:898	The successful fabrication route offers an excellent approach to design advanced strong integrated nacre-like composite materials, which can be applied in tissue engineering, protection, aerospace, and permeable membranes for separation and delivery.
26167700	4	27	from	hybrids	761:767	arg1	enhancement					727:737	the synergistic strengthening/toughening enhancement	686:737	the synergistic strengthening/toughening enhancement from the binary GO/SA hybrids	686:767	Both of the parameters are nearly many-times higher than those of similar materials because of the synergistic strengthening/toughening enhancement from the binary GO/SA hybrids.
26167700	3	28	theme	high	562:565	arg1	strength					567:574	high strength	562:574	high strength	562:574	Systematic evaluation for the mechanical property in different (dry/wet) environment/after thermal annealing shows a perfect combination of high strength and toughness.
26167700	1	29	theme	toughness	213:221	arg1	balance					189:195	the excellent balance	175:195	the excellent balance of strength and toughness	175:221	A crucial requirement for most engineering materials is the excellent balance of strength and toughness.
26167700	1	29	theme	toughness	213:221	arg1	requirement					129:139	A crucial requirement	119:139	A crucial requirement for most engineering materials	119:170	A crucial requirement for most engineering materials is the excellent balance of strength and toughness.
26167700	3	30	theme	toughness	580:588	arg1	combination					547:557	a perfect combination	537:557	a perfect combination of high strength and toughness	537:588	Systematic evaluation for the mechanical property in different (dry/wet) environment/after thermal annealing shows a perfect combination of high strength and toughness.
26167700	1	31	theme	crucial	121:127	arg1	requirement					129:139	A crucial requirement	119:139	A crucial requirement for most engineering materials	119:170	A crucial requirement for most engineering materials is the excellent balance of strength and toughness.
26167700	1	31	theme	crucial	121:127	arg1	balance					189:195	the excellent balance	175:195	the excellent balance of strength and toughness	175:221	A crucial requirement for most engineering materials is the excellent balance of strength and toughness.
26167700	5	32	theme	tissue	925:930	arg1	engineering					932:942	tissue engineering	925:942	tissue engineering	925:942	The successful fabrication route offers an excellent approach to design advanced strong integrated nacre-like composite materials, which can be applied in tissue engineering, protection, aerospace, and permeable membranes for separation and delivery.
26167700	5	33	theme	excellent	813:821	arg1	approach					823:830	an excellent approach	810:830	an excellent approach to design advanced strong integrated nacre-like composite materials	810:898	The successful fabrication route offers an excellent approach to design advanced strong integrated nacre-like composite materials, which can be applied in tissue engineering, protection, aerospace, and permeable membranes for separation and delivery.
26167700	3	34	theme	Systematic	422:431	arg1	evaluation					433:442	Systematic evaluation	422:442	Systematic evaluation for the mechanical property in different (dry/wet) environment/after thermal annealing	422:529	Systematic evaluation for the mechanical property in different (dry/wet) environment/after thermal annealing shows a perfect combination of high strength and toughness.
26167700	2	35	theme	hierarchical	248:259	arg1	structure					261:269	the hybrid hierarchical structure	237:269	the hybrid hierarchical structure in nacre	237:278	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	3	36	theme	mechanical	452:461	arg1	property					463:470	the mechanical property	448:470	the mechanical property in different (dry/wet) environment/after thermal annealing	448:529	Systematic evaluation for the mechanical property in different (dry/wet) environment/after thermal annealing shows a perfect combination of high strength and toughness.
26167700	0	37	theme	Nacre-like	60:69	arg1	Paper					112:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Binary Synergy Strengthening and Toughening of Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper.
26167700	2	38	theme	hybrid	241:246	arg1	structure					261:269	the hybrid hierarchical structure	237:269	the hybrid hierarchical structure in nacre	237:278	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	0	39	theme	Bio-Inspired	47:58	arg1	Paper					112:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Binary Synergy Strengthening and Toughening of Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper.
26167700	3	40	theme	different	475:483	arg1	annealing					521:529	different (dry/wet) environment/after thermal annealing	475:529	different (dry/wet) environment/after thermal annealing	475:529	Systematic evaluation for the mechanical property in different (dry/wet) environment/after thermal annealing shows a perfect combination of high strength and toughness.
26167700	3	41	from	property	463:470	arg1	annealing					521:529	different (dry/wet) environment/after thermal annealing	475:529	different (dry/wet) environment/after thermal annealing	475:529	Systematic evaluation for the mechanical property in different (dry/wet) environment/after thermal annealing shows a perfect combination of high strength and toughness.
26167700	4	42	theme	GO/SA	755:759	arg1	hybrids					761:767	the binary GO/SA hybrids	744:767	the binary GO/SA hybrids	744:767	Both of the parameters are nearly many-times higher than those of similar materials because of the synergistic strengthening/toughening enhancement from the binary GO/SA hybrids.
26167700	1	43	theme	most	145:148	arg1	materials					162:170	most engineering materials	145:170	most engineering materials	145:170	A crucial requirement for most engineering materials is the excellent balance of strength and toughness.
26167700	3	44	dep	different	475:483	arg1	dry/wet					486:492	dry/wet	486:492	dry/wet	486:492	Systematic evaluation for the mechanical property in different (dry/wet) environment/after thermal annealing shows a perfect combination of high strength and toughness.
26167700	3	44	dep	different	475:483	arg1	thermal					513:519	thermal	513:519	thermal	513:519	Systematic evaluation for the mechanical property in different (dry/wet) environment/after thermal annealing shows a perfect combination of high strength and toughness.
26167700	1	45	theme	engineering	150:160	arg1	materials					162:170	most engineering materials	145:170	most engineering materials	145:170	A crucial requirement for most engineering materials is the excellent balance of strength and toughness.
26167700	2	46	theme	oxide	340:344	arg1	blocks					381:386	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
26167700	0	47	theme	Oxide/Sodium	80:91	arg1	Paper					112:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Binary Synergy Strengthening and Toughening of Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper.
26167700	5	48	theme	composite	880:888	arg1	materials					890:898	advanced strong integrated nacre-like composite materials	842:898	advanced strong integrated nacre-like composite materials	842:898	The successful fabrication route offers an excellent approach to design advanced strong integrated nacre-like composite materials, which can be applied in tissue engineering, protection, aerospace, and permeable membranes for separation and delivery.
26167700	4	49	theme	synergistic	690:700	arg1	enhancement					727:737	the synergistic strengthening/toughening enhancement	686:737	the synergistic strengthening/toughening enhancement from the binary GO/SA hybrids	686:767	Both of the parameters are nearly many-times higher than those of similar materials because of the synergistic strengthening/toughening enhancement from the binary GO/SA hybrids.
26167700	4	50	theme	similar	657:663	arg1	materials					665:673	similar materials	657:673	similar materials	657:673	Both of the parameters are nearly many-times higher than those of similar materials because of the synergistic strengthening/toughening enhancement from the binary GO/SA hybrids.
26167700	0	51	theme	Graphene	71:78	arg1	Paper					112:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper	47:116	Binary Synergy Strengthening and Toughening of Bio-Inspired Nacre-like Graphene Oxide/Sodium Alginate Composite Paper.
26167700	5	52	theme	nacre-like	869:878	arg1	materials					890:898	advanced strong integrated nacre-like composite materials	842:898	advanced strong integrated nacre-like composite materials	842:898	The successful fabrication route offers an excellent approach to design advanced strong integrated nacre-like composite materials, which can be applied in tissue engineering, protection, aerospace, and permeable membranes for separation and delivery.
26167700	2	53	theme	alginate	358:365	arg1	blocks					381:386	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks	317:386	By mimicking the hybrid hierarchical structure in nacre, a kind of nacre-like paper based on binary hybrid graphene oxide (GO)/sodium alginate (SA) building blocks has been successfully fabricated.
28920399	0	0	theme	cartilage	98:106	arg1	scaffolds					108:116	artificial cartilage scaffolds	87:116	artificial cartilage scaffolds	87:116	[ATDC-5 growth promoted by sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds].
28920399	4	1	theme	U/L	705:707	arg1	lysozyme					709:716	10⁷ U/L lysozyme	701:716	10⁷ U/L lysozyme	701:716	When the microsphere was incubated under the condition of 10⁷ U/L lysozyme, the degradation rate was only 51.0%±1.8% on day 28.
28920399	6	2	theme	sustained-releasing	1228:1246	arg1	TGF-β1					1278:1283	the sustained-releasing chitosan microspheres loading TGF-β1	1224:1283	the sustained-releasing chitosan microspheres loading TGF-β1	1224:1283	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	1	3	theme	chondrocyte	154:164	arg1	ATDC-5					166:171	chondrocyte ATDC-5	154:171	chondrocyte ATDC-5	154:171	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	0	4	theme	artificial	87:96	arg1	scaffolds					108:116	artificial cartilage scaffolds	87:116	artificial cartilage scaffolds	87:116	[ATDC-5 growth promoted by sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds].
28920399	1	5	theme	ATDC-5	166:171	arg1	growth					144:149	the growth	140:149	the growth of chondrocyte ATDC-5	140:171	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	4	6	theme	%	753:753	arg1	rate					735:738	the degradation rate	719:738	the degradation rate	719:738	When the microsphere was incubated under the condition of 10⁷ U/L lysozyme, the degradation rate was only 51.0%±1.8% on day 28.
28920399	4	6	theme	%	753:753	arg1	%					758:758	51.0%±1.8%	749:758	51.0%±1.8%	749:758	When the microsphere was incubated under the condition of 10⁷ U/L lysozyme, the degradation rate was only 51.0%±1.8% on day 28.
28920399	1	7	theme	sustained-releasing	286:304	arg1	TGF-β1					336:341	the sustained-releasing chitosan microspheres loading TGF-β1	282:341	the sustained-releasing chitosan microspheres loading TGF-β1	282:341	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	6	8	theme	ATDC-5	1327:1332	arg1	growth					1317:1322	the growth	1313:1322	the growth of ATDC-5	1313:1332	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	1	9	theme	chitosan	306:313	arg1	TGF-β1					336:341	the sustained-releasing chitosan microspheres loading TGF-β1	282:341	the sustained-releasing chitosan microspheres loading TGF-β1	282:341	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	4	10	theme	lysozyme	709:716	arg1	condition					688:696	the condition	684:696	the condition of 10⁷ U/L lysozyme	684:716	When the microsphere was incubated under the condition of 10⁷ U/L lysozyme, the degradation rate was only 51.0%±1.8% on day 28.
28920399	5	11	theme	MTT	874:876	arg1	assay					878:882	MTT assay	874:882	MTT assay	874:882	Moreover, to compare the effect of TGF-β1, the growth of ATDC-5 in different scaffolds was observed by MTT assay and fluorescence staining test.
28920399	4	12	from	%	758:758	arg1	day					763:765	day 28	763:768	day 28	763:768	When the microsphere was incubated under the condition of 10⁷ U/L lysozyme, the degradation rate was only 51.0%±1.8% on day 28.
28920399	6	13	theme	cumulative	933:942	arg1	curve					952:956	the cumulative release curve	929:956	the cumulative release curve	929:956	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	1	14	theme	collagen	176:183	arg1	scaffolds					239:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds	176:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously	176:270	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	2	15	with	microspheres	475:486	arg1	TGF-β1					493:498	TGF-β1	493:498	TGF-β1	493:498	In addition, ATDC-5 was inoculated into the scaffolds incorporating the chitosan microspheres with TGF-β1.
28920399	6	16	theme	release	944:950	arg1	curve					952:956	the cumulative release curve	929:956	the cumulative release curve	929:956	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	5	17	theme	staining	901:908	arg1	test					910:913	fluorescence staining test	888:913	fluorescence staining test	888:913	Moreover, to compare the effect of TGF-β1, the growth of ATDC-5 in different scaffolds was observed by MTT assay and fluorescence staining test.
28920399	6	18	dep	released	970:977	arg1	reached					1040:1046	reached	1040:1046	reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5	1040:1332	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	1	19	theme	type	185:188	arg1	scaffolds					239:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds	176:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously	176:270	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	0	20	theme	[ATDC-5	0:6	arg1	growth					8:13	[ATDC-5 growth	0:13	[ATDC-5 growth	0:13	[ATDC-5 growth promoted by sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds].
28920399	1	21	theme	microspheres	315:326	arg1	TGF-β1					336:341	the sustained-releasing chitosan microspheres loading TGF-β1	282:341	the sustained-releasing chitosan microspheres loading TGF-β1	282:341	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	1	22	theme	II-hyaluronic	190:202	arg1	scaffolds					239:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds	176:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously	176:270	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	1	23	theme	loading	328:334	arg1	TGF-β1					336:341	the sustained-releasing chitosan microspheres loading TGF-β1	282:341	the sustained-releasing chitosan microspheres loading TGF-β1	282:341	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	6	24	theme	fluorescence	1087:1098	arg1	staining					1100:1107	fluorescence staining	1087:1107	fluorescence staining	1087:1107	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	1	25	theme	acid-chondroitin	204:219	arg1	scaffolds					239:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds	176:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously	176:270	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	3	26	theme	absorption	604:613	arg1	rate					615:618	absorption rate	604:618	absorption rate	604:618	Results show that the morphology of microsphere was round and uniform, mean diameter was about 100 nm, absorption rate was up to 983.7%±4.38%.
28920399	6	27	dep	assay	1077:1081	arg1	test					1109:1112	test	1109:1112	test	1109:1112	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	1	28	theme	sulfate	221:227	arg1	scaffolds					239:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds	176:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously	176:270	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	0	29	theme	chitosan	47:54	arg1	TGF-β1					77:82	sustained-releasing chitosan microspheres loading TGF-β1	27:82	sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds	27:116	[ATDC-5 growth promoted by sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds].
28920399	1	30	theme	composite	229:237	arg1	scaffolds					239:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds	176:247	collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously	176:270	In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking.
28920399	3	31	theme	983.7	630:634	arg1	%					635:635	%	635:635	%	635:635	Results show that the morphology of microsphere was round and uniform, mean diameter was about 100 nm, absorption rate was up to 983.7%±4.38%.
28920399	3	32	dep	%	635:635	arg1	to					627:628	to	627:628	to	627:628	Results show that the morphology of microsphere was round and uniform, mean diameter was about 100 nm, absorption rate was up to 983.7%±4.38%.
28920399	2	33	theme	chitosan	466:473	arg1	microspheres					475:486	the chitosan microspheres	462:486	the chitosan microspheres with TGF-β1	462:498	In addition, ATDC-5 was inoculated into the scaffolds incorporating the chitosan microspheres with TGF-β1.
28920399	0	34	theme	sustained-releasing	27:45	arg1	TGF-β1					77:82	sustained-releasing chitosan microspheres loading TGF-β1	27:82	sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds	27:116	[ATDC-5 growth promoted by sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds].
28920399	0	35	theme	loading	69:75	arg1	TGF-β1					77:82	sustained-releasing chitosan microspheres loading TGF-β1	27:82	sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds	27:116	[ATDC-5 growth promoted by sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds].
28920399	0	36	from	TGF-β1	77:82	arg1	scaffolds					108:116	artificial cartilage scaffolds	87:116	artificial cartilage scaffolds	87:116	[ATDC-5 growth promoted by sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds].
28920399	5	37	from	growth	818:823	arg1	scaffolds					848:856	different scaffolds	838:856	different scaffolds	838:856	Moreover, to compare the effect of TGF-β1, the growth of ATDC-5 in different scaffolds was observed by MTT assay and fluorescence staining test.
28920399	4	38	theme	degradation	723:733	arg1	rate					735:738	the degradation rate	719:738	the degradation rate	719:738	When the microsphere was incubated under the condition of 10⁷ U/L lysozyme, the degradation rate was only 51.0%±1.8% on day 28.
28920399	4	38	theme	degradation	723:733	arg1	%					758:758	51.0%±1.8%	749:758	51.0%±1.8%	749:758	When the microsphere was incubated under the condition of 10⁷ U/L lysozyme, the degradation rate was only 51.0%±1.8% on day 28.
28920399	0	39	theme	microspheres	56:67	arg1	TGF-β1					77:82	sustained-releasing chitosan microspheres loading TGF-β1	27:82	sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds	27:116	[ATDC-5 growth promoted by sustained-releasing chitosan microspheres loading TGF-β1 in artificial cartilage scaffolds].
28920399	6	40	theme	loading	1270:1276	arg1	TGF-β1					1278:1283	the sustained-releasing chitosan microspheres loading TGF-β1	1224:1283	the sustained-releasing chitosan microspheres loading TGF-β1	1224:1283	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	6	41	theme	MTT	1073:1075	arg1	assay					1077:1081	MTT assay	1073:1081	MTT assay	1073:1081	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	6	42	theme	microspheres	1257:1268	arg1	TGF-β1					1278:1283	the sustained-releasing chitosan microspheres loading TGF-β1	1224:1283	the sustained-releasing chitosan microspheres loading TGF-β1	1224:1283	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	6	43	dep	h.	1070:1071	arg1	demonstrated					1114:1125	demonstrated	1114:1125	demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5	1114:1332	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	6	44	theme	ATDC-5	1164:1169	arg1	growth					1171:1176	ATDC-5 growth	1164:1176	ATDC-5 growth	1164:1176	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	3	45	theme	microsphere	537:547	arg1	round					553:557	round	553:557	round	553:557	Results show that the morphology of microsphere was round and uniform, mean diameter was about 100 nm, absorption rate was up to 983.7%±4.38%.
28920399	3	45	theme	microsphere	537:547	arg1	morphology					523:532	the morphology	519:532	the morphology of microsphere	519:547	Results show that the morphology of microsphere was round and uniform, mean diameter was about 100 nm, absorption rate was up to 983.7%±4.38%.
28920399	5	46	theme	different	838:846	arg1	scaffolds					848:856	different scaffolds	838:856	different scaffolds	838:856	Moreover, to compare the effect of TGF-β1, the growth of ATDC-5 in different scaffolds was observed by MTT assay and fluorescence staining test.
28920399	6	47	theme	initial	990:996	arg1	h					1001:1001	initial 24 h	990:1001	initial 24 h	990:1001	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	6	48	theme	chitosan	1248:1255	arg1	TGF-β1					1278:1283	the sustained-releasing chitosan microspheres loading TGF-β1	1224:1283	the sustained-releasing chitosan microspheres loading TGF-β1	1224:1283	According to the cumulative release curve, TGF-β1 was released quickly at initial 24 h, then gradually decelerated, finally reached the plateau after 120 h. MTT assay and fluorescence staining test demonstrated that the scaffolds were suitable for ATDC-5 growth and proliferation, as well as, suggested that the sustained-releasing chitosan microspheres loading TGF-β1 could significantly promote the growth of ATDC-5.
28920399	5	49	theme	fluorescence	888:899	arg1	test					910:913	fluorescence staining test	888:913	fluorescence staining test	888:913	Moreover, to compare the effect of TGF-β1, the growth of ATDC-5 in different scaffolds was observed by MTT assay and fluorescence staining test.
28920399	3	50	theme	±4.38	636:640	arg1	%					635:635	%	635:635	%	635:635	Results show that the morphology of microsphere was round and uniform, mean diameter was about 100 nm, absorption rate was up to 983.7%±4.38%.
28920399	5	51	theme	TGF-β1	806:811	arg1	effect					796:801	the effect	792:801	the effect of TGF-β1	792:811	Moreover, to compare the effect of TGF-β1, the growth of ATDC-5 in different scaffolds was observed by MTT assay and fluorescence staining test.
28920399	3	52	theme	mean	572:575	arg1	diameter					577:584	mean diameter	572:584	mean diameter	572:584	Results show that the morphology of microsphere was round and uniform, mean diameter was about 100 nm, absorption rate was up to 983.7%±4.38%.
28920399	5	53	theme	ATDC-5	828:833	arg1	growth					818:823	the growth	814:823	the growth of ATDC-5 in different scaffolds	814:856	Moreover, to compare the effect of TGF-β1, the growth of ATDC-5 in different scaffolds was observed by MTT assay and fluorescence staining test.
28920399	4	54	theme	10⁷	701:703	arg1	lysozyme					709:716	10⁷ U/L lysozyme	701:716	10⁷ U/L lysozyme	701:716	When the microsphere was incubated under the condition of 10⁷ U/L lysozyme, the degradation rate was only 51.0%±1.8% on day 28.
28793792	5	0	from	pathways	825:832	arg1	turn					838:841	turn	838:841	turn	838:841	These signaling pathways, in turn, regulate the composition of ECM and its function.
28793792	1	1	from	Cells	74:78	arg1	organisms					97:105	multicellular organisms	83:105	multicellular organisms	83:105	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	10	2	dep	771-773	1194:1200	arg1	27					1190:1191	27	1190:1191	27	1190:1191	27, 771-773.
28793792	0	3	from	Matrix	14:19	arg1	Responses					53:61	Cellular Responses	44:61	Cellular Responses to Stress	44:71	Extracellular Matrix and Redox Signaling in Cellular Responses to Stress.
28793792	3	4	from	changes	525:531	arg1	ECM					540:542	the ECM	536:542	the ECM	536:542	This Forum addresses emerging mechanisms by which redox signaling controls and is controlled by changes in the ECM, focusing on the roles of matricellular proteins.
28793792	2	5	from	changes	297:303	arg1	modifications					371:383	post-translational modifications	352:383	post-translational modifications	352:383	Cells also reside in a structural extracellular matrix (ECM), and changes in its composition, mechanical properties, and post-translational modifications provide additional layers of communication.
28793792	2	5	from	changes	297:303	arg1	composition					312:322	its composition	308:322	its composition	308:322	Cells also reside in a structural extracellular matrix (ECM), and changes in its composition, mechanical properties, and post-translational modifications provide additional layers of communication.
28793792	2	5	from	changes	297:303	arg1	properties					336:345	mechanical properties	325:345	mechanical properties	325:345	Cells also reside in a structural extracellular matrix (ECM), and changes in its composition, mechanical properties, and post-translational modifications provide additional layers of communication.
28793792	4	6	dep	biosynthesis	704:715	arg1	the					700:702	the	700:702	the	700:702	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	6	7	theme	post-translational	903:920	arg1	modifications					922:934	Covalent post-translational modifications	894:934	Covalent post-translational modifications of ECM by redox molecules	894:960	Covalent post-translational modifications of ECM by redox molecules further regulate its structure and function.
28793792	7	8	theme	therapeutic	1139:1149	arg1	opportunities					1151:1163	new therapeutic opportunities	1135:1163	new therapeutic opportunities	1135:1163	Recent studies of acute injuries and chronic disease have identified important pathophysiological roles for this cross-talk and new therapeutic opportunities.
28793792	4	9	theme	transducers	796:806	arg1	biosynthesis					704:715	biosynthesis	704:715	biosynthesis	704:715	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	4	9	theme	transducers	796:806	arg1	activation					769:778	the activation	765:778	the activation of their signal transducers	765:806	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	4	9	theme	transducers	796:806	arg1	catabolism					721:730	catabolism	721:730	catabolism	721:730	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	3	10	theme	emerging	450:457	arg1	mechanisms					459:468	emerging mechanisms	450:468	emerging mechanisms by which redox signaling controls and is controlled by changes in the ECM	450:542	This Forum addresses emerging mechanisms by which redox signaling controls and is controlled by changes in the ECM, focusing on the roles of matricellular proteins.
28793792	6	11	theme	Covalent	894:901	arg1	modifications					922:934	Covalent post-translational modifications	894:934	Covalent post-translational modifications of ECM by redox molecules	894:960	Covalent post-translational modifications of ECM by redox molecules further regulate its structure and function.
28793792	4	12	theme	signal	789:794	arg1	transducers					796:806	their signal transducers	783:806	their signal transducers	783:806	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	2	13	theme	additional	393:402	arg1	layers					404:409	additional layers	393:409	additional layers of communication	393:426	Cells also reside in a structural extracellular matrix (ECM), and changes in its composition, mechanical properties, and post-translational modifications provide additional layers of communication.
28793792	0	14	from	Signaling	31:39	arg1	Responses					53:61	Cellular Responses	44:61	Cellular Responses to Stress	44:71	Extracellular Matrix and Redox Signaling in Cellular Responses to Stress.
28793792	0	15	theme	Extracellular	0:12	arg1	Matrix					14:19	Extracellular Matrix	0:19	Extracellular Matrix	0:19	Extracellular Matrix and Redox Signaling in Cellular Responses to Stress.
28793792	7	16	theme	new	1135:1137	arg1	opportunities					1151:1163	new therapeutic opportunities	1135:1163	new therapeutic opportunities	1135:1163	Recent studies of acute injuries and chronic disease have identified important pathophysiological roles for this cross-talk and new therapeutic opportunities.
28793792	1	17	theme	multicellular	83:95	arg1	organisms					97:105	multicellular organisms	83:105	multicellular organisms	83:105	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	1	18	theme	secreted	192:199	arg1	proteins					201:208	secreted proteins	192:208	secreted proteins	192:208	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	0	19	theme	Redox	25:29	arg1	Signaling					31:39	Redox Signaling	25:39	Redox Signaling	25:39	Extracellular Matrix and Redox Signaling in Cellular Responses to Stress.
28793792	4	20	theme	molecules	751:759	arg1	biosynthesis					704:715	biosynthesis	704:715	biosynthesis	704:715	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	4	20	theme	molecules	751:759	arg1	activation					769:778	the activation	765:778	the activation of their signal transducers	765:806	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	4	20	theme	molecules	751:759	arg1	catabolism					721:730	catabolism	721:730	catabolism	721:730	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	1	21	theme	proteins	201:208	arg1	variety					181:187	a variety	179:187	a variety of secreted proteins and small molecules	179:228	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	1	21	theme	proteins	201:208	arg1	proteins					201:208	secreted proteins	192:208	secreted proteins	192:208	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	1	21	theme	proteins	201:208	arg1	molecules					220:228	small molecules	214:228	small molecules	214:228	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	3	22	theme	matricellular	570:582	arg1	proteins					584:591	matricellular proteins	570:591	matricellular proteins	570:591	This Forum addresses emerging mechanisms by which redox signaling controls and is controlled by changes in the ECM, focusing on the roles of matricellular proteins.
28793792	9	23	theme	Redox	1176:1180	arg1	Signal					1182:1187	Redox Signal	1176:1187	Redox Signal.	1176:1188	Redox Signal.
28793792	4	24	theme	signaling	638:646	arg1	receptors					648:656	specific cell surface signaling receptors	616:656	specific cell surface signaling receptors	616:656	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	1	25	theme	small	214:218	arg1	molecules					220:228	small molecules	214:228	small molecules	214:228	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	5	26	theme	function	884:891	arg1	composition					857:867	the composition	853:867	the composition of ECM and its function	853:891	These signaling pathways, in turn, regulate the composition of ECM and its function.
28793792	2	27	theme	communication	414:426	arg1	layers					404:409	additional layers	393:409	additional layers of communication	393:426	Cells also reside in a structural extracellular matrix (ECM), and changes in its composition, mechanical properties, and post-translational modifications provide additional layers of communication.
28793792	5	28	theme	ECM	872:874	arg1	composition					857:867	the composition	853:867	the composition of ECM and its function	853:891	These signaling pathways, in turn, regulate the composition of ECM and its function.
28793792	7	29	theme	chronic	1044:1050	arg1	disease					1052:1058	chronic disease	1044:1058	chronic disease	1044:1058	Recent studies of acute injuries and chronic disease have identified important pathophysiological roles for this cross-talk and new therapeutic opportunities.
28793792	1	30	theme	molecules	220:228	arg1	variety					181:187	a variety	179:187	a variety of secreted proteins and small molecules	179:228	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	1	30	theme	molecules	220:228	arg1	proteins					201:208	secreted proteins	192:208	secreted proteins	192:208	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	1	30	theme	molecules	220:228	arg1	molecules					220:228	small molecules	214:228	small molecules	214:228	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	5	31	theme	signaling	815:823	arg1	pathways					825:832	These signaling pathways	809:832	These signaling pathways	809:832	These signaling pathways, in turn, regulate the composition of ECM and its function.
28793792	7	32	theme	important	1076:1084	arg1	roles					1105:1109	important pathophysiological roles	1076:1109	important pathophysiological roles for this cross-talk	1076:1129	Recent studies of acute injuries and chronic disease have identified important pathophysiological roles for this cross-talk and new therapeutic opportunities.
28793792	3	33	theme	proteins	584:591	arg1	roles					561:565	the roles	557:565	the roles of matricellular proteins	557:591	This Forum addresses emerging mechanisms by which redox signaling controls and is controlled by changes in the ECM, focusing on the roles of matricellular proteins.
28793792	7	34	theme	disease	1052:1058	arg1	studies					1014:1020	Recent studies	1007:1020	Recent studies of acute injuries and chronic disease	1007:1058	Recent studies of acute injuries and chronic disease have identified important pathophysiological roles for this cross-talk and new therapeutic opportunities.
28793792	2	35	theme	mechanical	325:334	arg1	properties					336:345	mechanical properties	325:345	mechanical properties	325:345	Cells also reside in a structural extracellular matrix (ECM), and changes in its composition, mechanical properties, and post-translational modifications provide additional layers of communication.
28793792	0	36	theme	Cellular	44:51	arg1	Responses					53:61	Cellular Responses	44:61	Cellular Responses to Stress	44:71	Extracellular Matrix and Redox Signaling in Cellular Responses to Stress.
28793792	7	37	theme	pathophysiological	1086:1103	arg1	roles					1105:1109	important pathophysiological roles	1076:1109	important pathophysiological roles for this cross-talk	1076:1129	Recent studies of acute injuries and chronic disease have identified important pathophysiological roles for this cross-talk and new therapeutic opportunities.
28793792	6	38	theme	ECM	939:941	arg1	modifications					922:934	Covalent post-translational modifications	894:934	Covalent post-translational modifications of ECM by redox molecules	894:960	Covalent post-translational modifications of ECM by redox molecules further regulate its structure and function.
28793792	7	39	theme	injuries	1031:1038	arg1	studies					1014:1020	Recent studies	1007:1020	Recent studies of acute injuries and chronic disease	1007:1058	Recent studies of acute injuries and chronic disease have identified important pathophysiological roles for this cross-talk and new therapeutic opportunities.
28793792	7	40	theme	acute	1025:1029	arg1	injuries					1031:1038	acute injuries	1025:1038	acute injuries	1025:1038	Recent studies of acute injuries and chronic disease have identified important pathophysiological roles for this cross-talk and new therapeutic opportunities.
28793792	2	41	theme	extracellular	265:277	arg1	ECM					287:289	ECM	287:289	ECM	287:289	Cells also reside in a structural extracellular matrix (ECM), and changes in its composition, mechanical properties, and post-translational modifications provide additional layers of communication.
28793792	2	41	theme	extracellular	265:277	arg1	matrix					279:284	a structural extracellular matrix	252:284	a structural extracellular matrix (ECM)	252:290	Cells also reside in a structural extracellular matrix (ECM), and changes in its composition, mechanical properties, and post-translational modifications provide additional layers of communication.
28793792	1	42	theme	neighboring	136:146	arg1	cells					148:152	neighboring cells	136:152	neighboring cells	136:152	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	4	43	theme	specific	616:623	arg1	receptors					648:656	specific cell surface signaling receptors	616:656	specific cell surface signaling receptors	616:656	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	2	44	theme	structural	254:263	arg1	ECM					287:289	ECM	287:289	ECM	287:289	Cells also reside in a structural extracellular matrix (ECM), and changes in its composition, mechanical properties, and post-translational modifications provide additional layers of communication.
28793792	2	44	theme	structural	254:263	arg1	matrix					279:284	a structural extracellular matrix	252:284	a structural extracellular matrix (ECM)	252:290	Cells also reside in a structural extracellular matrix (ECM), and changes in its composition, mechanical properties, and post-translational modifications provide additional layers of communication.
28793792	4	45	theme	redox	735:739	arg1	molecules					751:759	redox signaling molecules	735:759	redox signaling molecules	735:759	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	4	46	theme	signaling	741:749	arg1	molecules					751:759	redox signaling molecules	735:759	redox signaling molecules	735:759	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	3	47	theme	redox	479:483	arg1	signaling					485:493	redox signaling	479:493	redox signaling	479:493	This Forum addresses emerging mechanisms by which redox signaling controls and is controlled by changes in the ECM, focusing on the roles of matricellular proteins.
28793792	2	48	theme	post-translational	352:369	arg1	modifications					371:383	post-translational modifications	352:383	post-translational modifications	352:383	Cells also reside in a structural extracellular matrix (ECM), and changes in its composition, mechanical properties, and post-translational modifications provide additional layers of communication.
28793792	4	49	theme	surface	630:636	arg1	receptors					648:656	specific cell surface signaling receptors	616:656	specific cell surface signaling receptors	616:656	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
28793792	6	50	theme	redox	946:950	arg1	molecules					952:960	redox molecules	946:960	redox molecules	946:960	Covalent post-translational modifications of ECM by redox molecules further regulate its structure and function.
28793792	7	51	theme	Recent	1007:1012	arg1	studies					1014:1020	Recent studies	1007:1020	Recent studies of acute injuries and chronic disease	1007:1058	Recent studies of acute injuries and chronic disease have identified important pathophysiological roles for this cross-talk and new therapeutic opportunities.
28793792	1	52	theme	distant	158:164	arg1	organs					166:171	distant organs	158:171	distant organs	158:171	Cells in multicellular organisms communicate extensively with neighboring cells and distant organs using a variety of secreted proteins and small molecules.
28793792	4	53	theme	cell	625:628	arg1	receptors					648:656	specific cell surface signaling receptors	616:656	specific cell surface signaling receptors	616:656	These proteins engage specific cell surface signaling receptors, integrins, and proteoglycans to regulate the biosynthesis and catabolism of redox signaling molecules and the activation of their signal transducers.
23909489	3	0	theme	in	325:326	arg1	study					334:338	The current in vitro study	313:338	The current in vitro study conducted in a fermentation screening platform	313:385	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	6	1	from	decrease	945:952	arg1	levels					973:978	Bifidobacterium levels	957:978	Bifidobacterium levels	957:978	The combination GOS-Amoxicillin was especially of interest as, after decrease in Bifidobacterium levels, a recovery of mainly Bifidobacterium longum was observed and could be correlated with specific degradation patterns of GOS.
23909489	3	2	with	treatment	519:527	arg1	antibiotics					539:549	four antibiotics	534:549	four antibiotics at two doses	534:562	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	5	3	theme	particular	815:824	arg1	combinations					826:837	particular combinations	815:837	particular combinations of GOS antibiotic	815:855	This conclusion explains discrepancies in literature and indicates that particular combinations of GOS antibiotic should be studied.
23909489	3	4	from	impact	424:429	arg1	activity					505:512	activity	505:512	activity	505:512	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	3	4	from	impact	424:429	arg1	composition					489:499	adult gut microbiota composition	468:499	adult gut microbiota composition	468:499	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	8	5	theme	antibiotics	1402:1412	arg1	3					1395:1395	3	1395:1395	3	1395:1395	This showed that although GOS were utilized and beneficial bacteria could grow in 3 of 4 antibiotics tested, the metabolic activity of an antibiotic-treated microbiota was still disturbed as compared to the nontreated microbiota.
23909489	8	5	theme	antibiotics	1402:1412	arg1	antibiotics					1402:1412	4 antibiotics	1400:1412	4 antibiotics tested	1400:1419	This showed that although GOS were utilized and beneficial bacteria could grow in 3 of 4 antibiotics tested, the metabolic activity of an antibiotic-treated microbiota was still disturbed as compared to the nontreated microbiota.
23909489	3	6	from	doses	558:562	arg1	antibiotics					539:549	four antibiotics	534:549	four antibiotics at two doses	534:562	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	7	7	theme	monosaccharides	1182:1196	arg1	accumulation					1166:1177	an accumulation	1163:1177	an accumulation of monosaccharides and intermediate organic acids	1163:1227	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	3	8	theme	fermentation	355:366	arg1	platform					378:385	a fermentation screening platform	353:385	a fermentation screening platform	353:385	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	0	9	from	Impact	0:5	arg1	activity					83:90	metabolic activity	73:90	metabolic activity	73:90	Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation.
23909489	0	9	from	Impact	0:5	arg1	composition					57:67	gut microbiota composition	42:67	gut microbiota composition	42:67	Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation.
23909489	0	10	dep	in	125:126	arg1	vitro					128:132	vitro	128:132	vitro	128:132	Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation.
23909489	3	11	theme	screening	368:376	arg1	platform					378:385	a fermentation screening platform	353:385	a fermentation screening platform	353:385	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	1	12	contain	have	177:180	arg2	potential					182:190	potential	182:190	potential	182:190	Prebiotics are considered to have potential to reduce disturbances in the gut microbiota induced by antibiotics.
23909489	1	12	contain	have	177:180	arg1	Prebiotics					148:157	Prebiotics	148:157	Prebiotics	148:157	Prebiotics are considered to have potential to reduce disturbances in the gut microbiota induced by antibiotics.
23909489	4	13	theme	bacteria	602:609	arg1	abundance					589:597	relative abundance	580:597	relative abundance of bacteria	580:609	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	0	14	theme	in	125:126	arg1	fermentation					134:145	in vitro fermentation	125:145	in vitro fermentation	125:145	Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation.
23909489	5	15	theme	antibiotic	846:855	arg1	GOS					842:844	GOS antibiotic	842:855	GOS antibiotic	842:855	This conclusion explains discrepancies in literature and indicates that particular combinations of GOS antibiotic should be studied.
23909489	7	16	theme	acids	1223:1227	arg1	accumulation					1166:1177	an accumulation	1163:1177	an accumulation of monosaccharides and intermediate organic acids	1163:1227	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	8	17	theme	metabolic	1426:1434	arg1	activity					1436:1443	the metabolic activity	1422:1443	the metabolic activity of an antibiotic-treated microbiota	1422:1479	This showed that although GOS were utilized and beneficial bacteria could grow in 3 of 4 antibiotics tested, the metabolic activity of an antibiotic-treated microbiota was still disturbed as compared to the nontreated microbiota.
23909489	4	18	theme	Lactobacillus	675:687	arg1	changes					569:575	The changes	565:575	The changes in relative abundance of bacteria upon antibiotic treatment	565:635	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	4	18	theme	Lactobacillus	675:687	arg1	growth					645:650	the growth	641:650	the growth of Bifidobacterium and Lactobacillus upon GOS addition	641:705	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	4	18	theme	Lactobacillus	675:687	arg1	antibiotic					712:721	antibiotic	712:721	antibiotic	712:721	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	7	19	theme	antibiotic-treated	1245:1262	arg1	microbiota					1264:1273	antibiotic-treated microbiota	1245:1273	antibiotic-treated microbiota	1245:1273	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	6	20	theme	Bifidobacterium	957:971	arg1	levels					973:978	Bifidobacterium levels	957:978	Bifidobacterium levels	957:978	The combination GOS-Amoxicillin was especially of interest as, after decrease in Bifidobacterium levels, a recovery of mainly Bifidobacterium longum was observed and could be correlated with specific degradation patterns of GOS.
23909489	7	21	theme	individual	1147:1156	arg1	GOS					1158:1160	individual GOS	1147:1160	individual GOS	1147:1160	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	3	22	theme	galacto-oligosaccharides	434:457	arg1	impact					424:429	the impact	420:429	the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity	420:512	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	7	23	theme	GOS	1158:1160	arg1	profiles					1135:1142	different degradation profiles	1113:1142	different degradation profiles of individual GOS	1113:1160	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	4	24	theme	GOS	694:696	arg1	addition					698:705	GOS addition	694:705	GOS addition	694:705	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	4	25	from	changes	569:575	arg1	abundance					589:597	relative abundance	580:597	relative abundance of bacteria	580:609	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	3	26	dep	in	325:326	arg1	vitro					328:332	vitro	328:332	vitro	328:332	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	7	27	located	observed	1233:1240	arg2	accumulation					1166:1177	an accumulation	1163:1177	an accumulation of monosaccharides and intermediate organic acids	1163:1227	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	7	27	located	observed	1233:1240	arg1	microbiota					1264:1273	antibiotic-treated microbiota	1245:1273	antibiotic-treated microbiota	1245:1273	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	8	28	used	utilized	1348:1355	arg2	GOS					1339:1341	GOS	1339:1341	GOS	1339:1341	This showed that although GOS were utilized and beneficial bacteria could grow in 3 of 4 antibiotics tested, the metabolic activity of an antibiotic-treated microbiota was still disturbed as compared to the nontreated microbiota.
23909489	2	29	from	Results	261:267	arg1	literature					272:281	literature	272:281	literature	272:281	Results in literature are, however, not consistent.
23909489	3	30	theme	gut	474:476	arg1	composition					489:499	adult gut microbiota composition	468:499	adult gut microbiota composition	468:499	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	0	31	theme	galacto-oligosaccharides	10:33	arg1	Impact					0:5	Impact	0:5	Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation.	0:146	Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation.
23909489	4	32	theme	relative	580:587	arg1	abundance					589:597	relative abundance	580:597	relative abundance of bacteria	580:609	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	8	33	theme	antibiotic-treated	1451:1468	arg1	microbiota					1470:1479	an antibiotic-treated microbiota	1448:1479	an antibiotic-treated microbiota	1448:1479	This showed that although GOS were utilized and beneficial bacteria could grow in 3 of 4 antibiotics tested, the metabolic activity of an antibiotic-treated microbiota was still disturbed as compared to the nontreated microbiota.
23909489	3	34	theme	microbiota	478:487	arg1	composition					489:499	adult gut microbiota composition	468:499	adult gut microbiota composition	468:499	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	0	35	theme	gut	42:44	arg1	composition					57:67	gut microbiota composition	42:67	gut microbiota composition	42:67	Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation.
23909489	6	36	theme	longum	1018:1023	arg1	recovery					983:990	a recovery	981:990	a recovery of mainly Bifidobacterium longum	981:1023	The combination GOS-Amoxicillin was especially of interest as, after decrease in Bifidobacterium levels, a recovery of mainly Bifidobacterium longum was observed and could be correlated with specific degradation patterns of GOS.
23909489	7	37	theme	different	1113:1121	arg1	profiles					1135:1142	different degradation profiles	1113:1142	different degradation profiles of individual GOS	1113:1160	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	0	38	dep	composition	57:67	arg1	the					38:40	the	38:40	the	38:40	Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation.
23909489	4	39	from	growth	645:650	arg1	abundance					589:597	relative abundance	580:597	relative abundance of bacteria	580:609	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	1	40	theme	gut	222:224	arg1	microbiota					226:235	the gut microbiota	218:235	the gut microbiota induced by antibiotics	218:258	Prebiotics are considered to have potential to reduce disturbances in the gut microbiota induced by antibiotics.
23909489	7	41	theme	degradation	1123:1133	arg1	profiles					1135:1142	different degradation profiles	1113:1142	different degradation profiles of individual GOS	1113:1160	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	3	42	theme	adult	468:472	arg1	composition					489:499	adult gut microbiota composition	468:499	adult gut microbiota composition	468:499	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	5	43	theme	GOS	842:844	arg1	combinations					826:837	particular combinations	815:837	particular combinations of GOS antibiotic	815:855	This conclusion explains discrepancies in literature and indicates that particular combinations of GOS antibiotic should be studied.
23909489	6	44	theme	GOS	1100:1102	arg1	patterns					1088:1095	specific degradation patterns	1067:1095	specific degradation patterns of GOS	1067:1102	The combination GOS-Amoxicillin was especially of interest as, after decrease in Bifidobacterium levels, a recovery of mainly Bifidobacterium longum was observed and could be correlated with specific degradation patterns of GOS.
23909489	5	45	from	discrepancies	768:780	arg1	literature					785:794	literature	785:794	literature	785:794	This conclusion explains discrepancies in literature and indicates that particular combinations of GOS antibiotic should be studied.
23909489	0	46	theme	microbiota	46:55	arg1	composition					57:67	gut microbiota composition	42:67	gut microbiota composition	42:67	Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation.
23909489	3	47	theme	current	317:323	arg1	study					334:338	The current in vitro study	313:338	The current in vitro study conducted in a fermentation screening platform	313:385	The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses.
23909489	7	48	theme	nontreated	1290:1299	arg1	microbiota					1301:1310	nontreated microbiota	1290:1310	nontreated microbiota	1290:1310	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	7	49	theme	organic	1215:1221	arg1	acids					1223:1227	intermediate organic acids	1202:1227	intermediate organic acids	1202:1227	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	8	50	theme	microbiota	1470:1479	arg1	activity					1436:1443	the metabolic activity	1422:1443	the metabolic activity of an antibiotic-treated microbiota	1422:1479	This showed that although GOS were utilized and beneficial bacteria could grow in 3 of 4 antibiotics tested, the metabolic activity of an antibiotic-treated microbiota was still disturbed as compared to the nontreated microbiota.
23909489	8	51	theme	beneficial	1361:1370	arg1	bacteria					1372:1379	beneficial bacteria	1361:1379	beneficial bacteria	1361:1379	This showed that although GOS were utilized and beneficial bacteria could grow in 3 of 4 antibiotics tested, the metabolic activity of an antibiotic-treated microbiota was still disturbed as compared to the nontreated microbiota.
23909489	6	52	theme	combination	880:890	arg1	GOS-Amoxicillin					892:906	The combination GOS-Amoxicillin	876:906	The combination GOS-Amoxicillin	876:906	The combination GOS-Amoxicillin was especially of interest as, after decrease in Bifidobacterium levels, a recovery of mainly Bifidobacterium longum was observed and could be correlated with specific degradation patterns of GOS.
23909489	4	53	theme	antibiotic	616:625	arg1	treatment					627:635	antibiotic treatment	616:635	antibiotic treatment	616:635	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	0	54	theme	metabolic	73:81	arg1	activity					83:90	metabolic activity	73:90	metabolic activity	73:90	Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation.
23909489	4	55	theme	Bifidobacterium	655:669	arg1	changes					569:575	The changes	565:575	The changes in relative abundance of bacteria upon antibiotic treatment	565:635	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	4	55	theme	Bifidobacterium	655:669	arg1	growth					645:650	the growth	641:650	the growth of Bifidobacterium and Lactobacillus upon GOS addition	641:705	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	4	55	theme	Bifidobacterium	655:669	arg1	antibiotic					712:721	antibiotic	712:721	antibiotic	712:721	The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependent.
23909489	8	56	theme	nontreated	1520:1529	arg1	microbiota					1531:1540	the nontreated microbiota	1516:1540	the nontreated microbiota	1516:1540	This showed that although GOS were utilized and beneficial bacteria could grow in 3 of 4 antibiotics tested, the metabolic activity of an antibiotic-treated microbiota was still disturbed as compared to the nontreated microbiota.
23909489	1	57	from	disturbances	202:213	arg1	microbiota					226:235	the gut microbiota	218:235	the gut microbiota induced by antibiotics	218:258	Prebiotics are considered to have potential to reduce disturbances in the gut microbiota induced by antibiotics.
23909489	6	58	theme	degradation	1076:1086	arg1	patterns					1088:1095	specific degradation patterns	1067:1095	specific degradation patterns of GOS	1067:1102	The combination GOS-Amoxicillin was especially of interest as, after decrease in Bifidobacterium levels, a recovery of mainly Bifidobacterium longum was observed and could be correlated with specific degradation patterns of GOS.
23909489	7	59	theme	intermediate	1202:1213	arg1	acids					1223:1227	intermediate organic acids	1202:1227	intermediate organic acids	1202:1227	Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota.
23909489	6	60	theme	specific	1067:1074	arg1	patterns					1088:1095	specific degradation patterns	1067:1095	specific degradation patterns of GOS	1067:1102	The combination GOS-Amoxicillin was especially of interest as, after decrease in Bifidobacterium levels, a recovery of mainly Bifidobacterium longum was observed and could be correlated with specific degradation patterns of GOS.
23909489	0	61	theme	antibiotic	97:106	arg1	treatment					108:116	antibiotic treatment	97:116	antibiotic treatment	97:116	Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation.
24183682	0	0	theme	immune	53:58	arg1	evolution					60:68	physical, chemical, and immune evolution	29:68	physical, chemical, and immune evolution from partum to 90 days postpartum	29:102	From goat colostrum to milk: physical, chemical, and immune evolution from partum to 90 days postpartum.
24183682	2	1	theme	length	389:394	arg1	size					304:307	a litter size	295:307	a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d)	295:406	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	4	2	theme	somatic	684:690	arg1	count					697:701	somatic cell count	684:701	somatic cell count	684:701	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	2	3	from	average	324:330	arg1	breed					340:344	this breed	335:344	this breed is 1.83 prolificacy	335:364	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	2	4	theme	gestation	379:387	arg1	d					405:405	149 ± 1 d	397:405	149 ± 1 d	397:405	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	2	4	theme	gestation	379:387	arg1	length					389:394	similar gestation length	371:394	similar gestation length (149 ± 1 d)	371:406	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	9	5	theme	other	1274:1278	arg1	hand					1280:1283	the other hand	1270:1283	the other hand	1270:1283	On the other hand, this study might be used to establish what the best use is: processing or kid feeding.
24183682	4	6	theme	cell	692:695	arg1	count					697:701	somatic cell count	684:701	somatic cell count	684:701	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	2	7	used	used	413:416	arg2	goats					237:241	Ten multiparous Majorera goats	212:241	Ten multiparous Majorera goats	212:241	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	7	8	theme	cell	987:990	arg1	count					992:996	somatic cell count	979:996	somatic cell count	979:996	Density, titratable acidity, rennet clotting time, and somatic cell count decreased throughout the lactation period, whereas pH and ethanol stability increased.
24183682	9	9	used	used	1306:1309	arg2	study					1291:1295	this study	1286:1295	this study	1286:1295	On the other hand, this study might be used to establish what the best use is: processing or kid feeding.
24183682	2	10	theme	Majorera	228:235	arg1	goats					237:241	Ten multiparous Majorera goats	212:241	Ten multiparous Majorera goats	212:241	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	5	11	theme	IgM	748:750	arg1	concentrations					752:765	IgG, IgA, and IgM concentrations	734:765	IgG, IgA, and IgM concentrations	734:765	Moreover, immunity molecules (IgG, IgA, and IgM concentrations and chitotriosidase activity) received great attention.
24183682	9	12	theme	best	1333:1336	arg1	use					1338:1340	the best use	1329:1340	the best use	1329:1340	On the other hand, this study might be used to establish what the best use is: processing or kid feeding.
24183682	3	13	theme	Goat	419:422	arg1	kids					424:427	Goat kids	419:427	Goat kids	419:427	Goat kids were removed from their dams to avoid interferences with the study.
24183682	2	14	theme	multiparous	216:226	arg1	goats					237:241	Ten multiparous Majorera goats	212:241	Ten multiparous Majorera goats	212:241	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	1	15	theme	lactation	201:209	arg1	d					193:193	d 90	193:196	d 90 of lactation	193:209	This study focused on the study of the changes originated in the milk from partum until d 90 of lactation.
24183682	2	16	theme	1	403:403	arg1	±					401:401	±	401:401	±	401:401	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	2	17	dep	breed	340:344	arg1	prolificacy					354:364	1.83 prolificacy	349:364	1.83 prolificacy	349:364	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	5	18	theme	chitotriosidase	771:785	arg1	activity					787:794	chitotriosidase activity	771:794	chitotriosidase activity	771:794	Moreover, immunity molecules (IgG, IgA, and IgM concentrations and chitotriosidase activity) received great attention.
24183682	3	19	attach	removed	434:440	arg2	kids					424:427	Goat kids	419:427	Goat kids	419:427	Goat kids were removed from their dams to avoid interferences with the study.
24183682	3	19	attach	removed	434:440	arg1	dams					453:456	their dams	447:456	their dams	447:456	Goat kids were removed from their dams to avoid interferences with the study.
24183682	2	20	theme	kids	314:317	arg1	size					304:307	a litter size	295:307	a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d)	295:406	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	7	21	theme	somatic	979:985	arg1	count					992:996	somatic cell count	979:996	somatic cell count	979:996	Density, titratable acidity, rennet clotting time, and somatic cell count decreased throughout the lactation period, whereas pH and ethanol stability increased.
24183682	5	22	theme	immunity	714:721	arg1	molecules					723:731	immunity molecules	714:731	immunity molecules (IgG, IgA, and IgM concentrations and chitotriosidase activity)	714:795	Moreover, immunity molecules (IgG, IgA, and IgM concentrations and chitotriosidase activity) received great attention.
24183682	4	23	theme	rennet	658:663	arg1	time					674:677	rennet clotting time	658:677	rennet clotting time	658:677	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	0	24	dep	evolution	60:68	arg1	colostrum					10:18	goat colostrum	5:18	goat colostrum to milk	5:26	From goat colostrum to milk: physical, chemical, and immune evolution from partum to 90 days postpartum.
24183682	6	25	theme	days	872:875	arg1	postpartum					877:886	the first days postpartum	862:886	the first days postpartum	862:886	Fat and protein content were higher in the first days postpartum, whereas lactose content was lower.
24183682	0	26	theme	goat	5:8	arg1	colostrum					10:18	goat colostrum	5:18	goat colostrum to milk	5:26	From goat colostrum to milk: physical, chemical, and immune evolution from partum to 90 days postpartum.
24183682	3	27	with	interferences	467:479	arg1	study					490:494	the study	486:494	the study	486:494	Goat kids were removed from their dams to avoid interferences with the study.
24183682	5	28	theme	IgA	739:741	arg1	concentrations					752:765	IgG, IgA, and IgM concentrations	734:765	IgG, IgA, and IgM concentrations	734:765	Moreover, immunity molecules (IgG, IgA, and IgM concentrations and chitotriosidase activity) received great attention.
24183682	6	29	theme	first	866:870	arg1	postpartum					877:886	the first days postpartum	862:886	the first days postpartum	862:886	Fat and protein content were higher in the first days postpartum, whereas lactose content was lower.
24183682	8	30	theme	newborn	1253:1259	arg1	kids					1261:1264	the newborn kids	1249:1264	the newborn kids	1249:1264	Relative to the immunological parameters, each measured parameter obtained its maximum level at d 0, showing the first milking as the choice to provide immunity to the newborn kids.
24183682	4	31	theme	clotting	665:672	arg1	time					674:677	rennet clotting time	658:677	rennet clotting time	658:677	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	7	32	theme	rennet	953:958	arg1	time					969:972	rennet clotting time	953:972	rennet clotting time	953:972	Density, titratable acidity, rennet clotting time, and somatic cell count decreased throughout the lactation period, whereas pH and ethanol stability increased.
24183682	5	33	theme	great	806:810	arg1	attention					812:820	great attention	806:820	great attention	806:820	Moreover, immunity molecules (IgG, IgA, and IgM concentrations and chitotriosidase activity) received great attention.
24183682	0	34	theme	days	88:91	arg1	postpartum					93:102	90 days postpartum	85:102	90 days postpartum	85:102	From goat colostrum to milk: physical, chemical, and immune evolution from partum to 90 days postpartum.
24183682	1	35	theme	changes	144:150	arg1	study					131:135	the study	127:135	the study of the changes originated in the milk from partum until d 90 of lactation	127:209	This study focused on the study of the changes originated in the milk from partum until d 90 of lactation.
24183682	7	36	theme	clotting	960:967	arg1	time					969:972	rennet clotting time	953:972	rennet clotting time	953:972	Density, titratable acidity, rennet clotting time, and somatic cell count decreased throughout the lactation period, whereas pH and ethanol stability increased.
24183682	5	37	dep	molecules	723:731	arg1	concentrations					752:765	IgG, IgA, and IgM concentrations	734:765	IgG, IgA, and IgM concentrations	734:765	Moreover, immunity molecules (IgG, IgA, and IgM concentrations and chitotriosidase activity) received great attention.
24183682	5	37	dep	molecules	723:731	arg1	activity					787:794	chitotriosidase activity	771:794	chitotriosidase activity	771:794	Moreover, immunity molecules (IgG, IgA, and IgM concentrations and chitotriosidase activity) received great attention.
24183682	4	38	theme	other	578:582	arg1	acidity					630:636	titratable acidity	619:636	titratable acidity	619:636	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	4	38	theme	other	578:582	arg1	time					674:677	rennet clotting time	658:677	rennet clotting time	658:677	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	4	38	theme	other	578:582	arg1	pH					606:607	pH	606:607	pH	606:607	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	4	38	theme	other	578:582	arg1	stability					647:655	ethanol stability	639:655	ethanol stability	639:655	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	4	38	theme	other	578:582	arg1	density					610:616	density	610:616	density	610:616	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	4	38	theme	other	578:582	arg1	properties					584:593	some other properties	573:593	some other properties	573:593	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	4	38	theme	other	578:582	arg1	count					697:701	somatic cell count	684:701	somatic cell count	684:701	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	2	39	theme	similar	371:377	arg1	d					405:405	149 ± 1 d	397:405	149 ± 1 d	397:405	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	2	39	theme	similar	371:377	arg1	length					389:394	similar gestation length	371:394	similar gestation length (149 ± 1 d)	371:406	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	4	40	theme	ethanol	639:645	arg1	stability					647:655	ethanol stability	639:655	ethanol stability	639:655	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	2	41	with	goats	237:241	arg1	size					304:307	a litter size	295:307	a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d)	295:406	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	4	42	dep	content	511:517	arg1	protein					525:531	protein	525:531	protein	525:531	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	4	42	dep	content	511:517	arg1	lactose					538:544	lactose	538:544	lactose	538:544	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	4	42	dep	content	511:517	arg1	fat					520:522	fat	520:522	fat	520:522	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	1	43	from	partum	180:185	arg1	milk					170:173	the milk	166:173	the milk from partum until d 90 of lactation	166:209	This study focused on the study of the changes originated in the milk from partum until d 90 of lactation.
24183682	7	44	theme	lactation	1023:1031	arg1	period					1033:1038	the lactation period	1019:1038	the lactation period	1019:1038	Density, titratable acidity, rennet clotting time, and somatic cell count decreased throughout the lactation period, whereas pH and ethanol stability increased.
24183682	8	45	theme	maximum	1164:1170	arg1	level					1172:1176	its maximum level	1160:1176	its maximum level	1160:1176	Relative to the immunological parameters, each measured parameter obtained its maximum level at d 0, showing the first milking as the choice to provide immunity to the newborn kids.
24183682	9	46	dep	used	1306:1309	arg1	kid					1360:1362	kid	1360:1362	kid	1360:1362	On the other hand, this study might be used to establish what the best use is: processing or kid feeding.
24183682	9	46	dep	used	1306:1309	arg1	processing					1346:1355	processing	1346:1355	processing	1346:1355	On the other hand, this study might be used to establish what the best use is: processing or kid feeding.
24183682	0	47	theme	physical	29:36	arg1	evolution					60:68	physical, chemical, and immune evolution	29:68	physical, chemical, and immune evolution from partum to 90 days postpartum	29:102	From goat colostrum to milk: physical, chemical, and immune evolution from partum to 90 days postpartum.
24183682	7	48	dep	decreased	998:1006	arg1	whereas					1041:1047	whereas	1041:1047	whereas	1041:1047	Density, titratable acidity, rennet clotting time, and somatic cell count decreased throughout the lactation period, whereas pH and ethanol stability increased.
24183682	2	49	theme	health	272:277	arg1	standards					279:287	animal health standards	265:287	animal health standards	265:287	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	0	50	from	partum	75:80	arg1	evolution					60:68	physical, chemical, and immune evolution	29:68	physical, chemical, and immune evolution from partum to 90 days postpartum	29:102	From goat colostrum to milk: physical, chemical, and immune evolution from partum to 90 days postpartum.
24183682	7	51	theme	titratable	933:942	arg1	acidity					944:950	titratable acidity	933:950	titratable acidity	933:950	Density, titratable acidity, rennet clotting time, and somatic cell count decreased throughout the lactation period, whereas pH and ethanol stability increased.
24183682	2	52	theme	animal	265:270	arg1	standards					279:287	animal health standards	265:287	animal health standards	265:287	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	6	53	theme	protein	831:837	arg1	content					839:845	protein content	831:845	protein content	831:845	Fat and protein content were higher in the first days postpartum, whereas lactose content was lower.
24183682	8	54	theme	first	1198:1202	arg1	milking					1204:1210	the first milking	1194:1210	the first milking as the choice to provide immunity to the newborn kids	1194:1264	Relative to the immunological parameters, each measured parameter obtained its maximum level at d 0, showing the first milking as the choice to provide immunity to the newborn kids.
24183682	8	55	theme	immunological	1101:1113	arg1	parameters					1115:1124	the immunological parameters	1097:1124	the immunological parameters	1097:1124	Relative to the immunological parameters, each measured parameter obtained its maximum level at d 0, showing the first milking as the choice to provide immunity to the newborn kids.
24183682	8	56	theme	measured	1132:1139	arg1	parameter					1141:1149	each measured parameter	1127:1149	each measured parameter	1127:1149	Relative to the immunological parameters, each measured parameter obtained its maximum level at d 0, showing the first milking as the choice to provide immunity to the newborn kids.
24183682	5	57	theme	IgG	734:736	arg1	concentrations					752:765	IgG, IgA, and IgM concentrations	734:765	IgG, IgA, and IgM concentrations	734:765	Moreover, immunity molecules (IgG, IgA, and IgM concentrations and chitotriosidase activity) received great attention.
24183682	2	58	theme	149	397:399	arg1	±					401:401	±	401:401	±	401:401	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	7	59	dep	pH	1049:1050	arg1	stability					1064:1072	stability	1064:1072	stability	1064:1072	Density, titratable acidity, rennet clotting time, and somatic cell count decreased throughout the lactation period, whereas pH and ethanol stability increased.
24183682	2	60	dep	kids	314:317	arg1	average					324:330	the average	320:330	the average in this breed is 1.83 prolificacy	320:364	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	4	61	theme	Compositional	497:509	arg1	content					511:517	Compositional content	497:517	Compositional content (fat, protein, and lactose)	497:545	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	2	62	theme	litter	297:302	arg1	size					304:307	a litter size	295:307	a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d)	295:406	Ten multiparous Majorera goats, bred carefully under animal health standards, with a litter size of 2 kids (the average in this breed is 1.83 prolificacy) and similar gestation length (149 ± 1 d) were used.
24183682	9	63	dep	processing	1346:1355	arg1	feeding					1364:1370	feeding	1364:1370	feeding	1364:1370	On the other hand, this study might be used to establish what the best use is: processing or kid feeding.
24183682	0	64	theme	chemical	39:46	arg1	evolution					60:68	physical, chemical, and immune evolution	29:68	physical, chemical, and immune evolution from partum to 90 days postpartum	29:102	From goat colostrum to milk: physical, chemical, and immune evolution from partum to 90 days postpartum.
24183682	4	65	theme	titratable	619:628	arg1	acidity					630:636	titratable acidity	619:636	titratable acidity	619:636	Compositional content (fat, protein, and lactose) were measured, as well as some other properties, including pH, density, titratable acidity, ethanol stability, rennet clotting time, and somatic cell count.
24183682	6	66	theme	lactose	897:903	arg1	content					905:911	lactose content	897:911	lactose content	897:911	Fat and protein content were higher in the first days postpartum, whereas lactose content was lower.
26454048	0	0	theme	alveolar	110:117	arg1	regeneration					124:135	alveolar bone regeneration	110:135	alveolar bone regeneration	110:135	Hydroxyapatite-calcium sulfate-hyaluronic acid composite encapsulated with collagenase as bone substitute for alveolar bone regeneration.
26454048	6	1	theme	histological	994:1005	arg1	examination					1007:1017	histological examination	994:1017	histological examination	994:1017	The in vivo bone formation was assessed in rat with alveolar bone defects and the bone augmentation by the HAP/CS/HA-Col composite was confirmed by micro-CT images and histological examination.
26454048	8	2	theme	collagenase	1138:1148	arg1	profile					1158:1164	The collagenase release profile	1134:1164	The collagenase release profile	1134:1164	The collagenase release profile had facilitated the acceleration of bone remodeling process and it was confirmed by the findings of micro-CT and H&E staining.
26454048	10	3	theme	collagenase	1609:1619	arg1	approach					1668:1675	a feasible approach	1657:1675	a feasible approach for the alveolar bone regeneration	1657:1710	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	10	3	theme	collagenase	1609:1619	arg1	inclusion					1621:1629	the collagenase inclusion	1605:1629	the collagenase inclusion in HAP/CS/HA composite	1605:1652	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	2	4	theme	teeth	390:394	arg1	reparability					370:381	limited reparability	362:381	limited reparability of the teeth	362:394	The bone loss is naturally irreversible because of limited reparability of the teeth.
26454048	10	5	theme	feasible	1659:1666	arg1	approach					1668:1675	a feasible approach	1657:1675	a feasible approach for the alveolar bone regeneration	1657:1710	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	10	5	theme	feasible	1659:1666	arg1	inclusion					1621:1629	the collagenase inclusion	1605:1629	the collagenase inclusion in HAP/CS/HA composite	1605:1652	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	9	6	theme	hydroxyapatite	1536:1549	arg1	granules					1559:1566	the collagenase and the porous hydroxyapatite (p-HAP) granules	1505:1566	the collagenase and the porous hydroxyapatite (p-HAP) granules	1505:1566	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	9	7	theme	bone	1407:1410	arg1	formation					1412:1420	improved new bone formation	1394:1420	improved new bone formation with matured bone morphology	1394:1449	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	7	8	theme	excellent	1061:1069	arg1	biocompatibility					1071:1086	excellent biocompatibility	1061:1086	excellent biocompatibility	1061:1086	The mechanical strength of 6.69 MPa with excellent biocompatibility was obtained for the HAP/CS/HA-Col composite.
26454048	10	9	theme	said	1573:1576	arg1	findings					1578:1585	The said findings	1569:1585	The said findings	1569:1585	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	9	10	theme	2 mg/mL	1356:1362	arg1	collagenase					1371:1381	HAP/CS/HA composite containing 2 mg/mL type I collagenase	1325:1381	HAP/CS/HA composite containing 2 mg/mL type I collagenase	1325:1381	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	5	11	theme	composite	728:736	arg1	material					738:745	The composite material	724:745	The composite material	724:745	The composite material was mechanically tested and the biocompatibility was evaluated by WST-1 assay.
26454048	6	12	theme	bone	887:890	arg1	defects					892:898	alveolar bone defects	878:898	alveolar bone defects	878:898	The in vivo bone formation was assessed in rat with alveolar bone defects and the bone augmentation by the HAP/CS/HA-Col composite was confirmed by micro-CT images and histological examination.
26454048	0	13	theme	bone	119:122	arg1	regeneration					124:135	alveolar bone regeneration	110:135	alveolar bone regeneration	110:135	Hydroxyapatite-calcium sulfate-hyaluronic acid composite encapsulated with collagenase as bone substitute for alveolar bone regeneration.
26454048	9	14	theme	matured	1427:1433	arg1	morphology					1440:1449	matured bone morphology	1427:1449	matured bone morphology	1427:1449	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	8	15	theme	release	1150:1156	arg1	profile					1158:1164	The collagenase release profile	1134:1164	The collagenase release profile	1134:1164	The collagenase release profile had facilitated the acceleration of bone remodeling process and it was confirmed by the findings of micro-CT and H&E staining.
26454048	6	16	theme	alveolar	878:885	arg1	defects					892:898	alveolar bone defects	878:898	alveolar bone defects	878:898	The in vivo bone formation was assessed in rat with alveolar bone defects and the bone augmentation by the HAP/CS/HA-Col composite was confirmed by micro-CT images and histological examination.
26454048	2	17	theme	bone	315:318	arg1	loss					320:323	The bone loss	311:323	The bone loss	311:323	The bone loss is naturally irreversible because of limited reparability of the teeth.
26454048	2	17	theme	bone	315:318	arg1	irreversible					338:349	irreversible	338:349	irreversible	338:349	The bone loss is naturally irreversible because of limited reparability of the teeth.
26454048	8	18	theme	staining	1283:1290	arg1	findings					1254:1261	the findings	1250:1261	the findings of micro-CT and H&E staining	1250:1290	The collagenase release profile had facilitated the acceleration of bone remodeling process and it was confirmed by the findings of micro-CT and H&E staining.
26454048	9	19	theme	bone	1435:1438	arg1	morphology					1440:1449	matured bone morphology	1427:1449	matured bone morphology	1427:1449	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	3	20	theme	tissue-engineered	518:534	arg1	constructs					536:545	tissue-engineered constructs	518:545	tissue-engineered constructs	518:545	Advancement in tissue engineering provides an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs.
26454048	3	21	with	regeneration	453:464	arg1	implants					506:513	suitable dental implants	490:513	suitable dental implants	490:513	Advancement in tissue engineering provides an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs.
26454048	3	21	with	regeneration	453:464	arg1	constructs					536:545	tissue-engineered constructs	518:545	tissue-engineered constructs	518:545	Advancement in tissue engineering provides an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs.
26454048	1	22	theme	periodontium	199:210	arg1	condition					182:190	a very severe inflammatory condition	155:190	a very severe inflammatory condition of the periodontium that progressively damages the soft tissue and destroys the alveolar bone that supports the teeth	155:308	Periodontitis is a very severe inflammatory condition of the periodontium that progressively damages the soft tissue and destroys the alveolar bone that supports the teeth.
26454048	1	22	theme	periodontium	199:210	arg1	Periodontitis					138:150	Periodontitis	138:150	Periodontitis	138:150	Periodontitis is a very severe inflammatory condition of the periodontium that progressively damages the soft tissue and destroys the alveolar bone that supports the teeth.
26454048	8	23	theme	H&E	1279:1281	arg1	staining					1283:1290	H&E staining	1279:1290	H&E staining	1279:1290	The collagenase release profile had facilitated the acceleration of bone remodeling process and it was confirmed by the findings of micro-CT and H&E staining.
26454048	7	24	theme	mechanical	1024:1033	arg1	strength					1035:1042	The mechanical strength	1020:1042	The mechanical strength of 6.69 MPa with excellent biocompatibility	1020:1086	The mechanical strength of 6.69 MPa with excellent biocompatibility was obtained for the HAP/CS/HA-Col composite.
26454048	3	25	theme	suitable	490:497	arg1	implants					506:513	suitable dental implants	490:513	suitable dental implants	490:513	Advancement in tissue engineering provides an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs.
26454048	0	26	theme	sulfate-hyaluronic	23:40	arg1	composite					47:55	Hydroxyapatite-calcium sulfate-hyaluronic acid composite	0:55	Hydroxyapatite-calcium sulfate-hyaluronic acid composite encapsulated with collagenase as bone	0:93	Hydroxyapatite-calcium sulfate-hyaluronic acid composite encapsulated with collagenase as bone substitute for alveolar bone regeneration.
26454048	3	27	theme	osseous	469:475	arg1	defects					477:483	osseous defects	469:483	osseous defects	469:483	Advancement in tissue engineering provides an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs.
26454048	0	28	theme	Hydroxyapatite-calcium	0:21	arg1	composite					47:55	Hydroxyapatite-calcium sulfate-hyaluronic acid composite	0:55	Hydroxyapatite-calcium sulfate-hyaluronic acid composite encapsulated with collagenase as bone	0:93	Hydroxyapatite-calcium sulfate-hyaluronic acid composite encapsulated with collagenase as bone substitute for alveolar bone regeneration.
26454048	9	29	theme	HAP/CS/HA	1325:1333	arg1	collagenase					1371:1381	HAP/CS/HA composite containing 2 mg/mL type I collagenase	1325:1381	HAP/CS/HA composite containing 2 mg/mL type I collagenase	1325:1381	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	3	30	theme	defects	477:483	arg1	regeneration					453:464	an effective regeneration	440:464	an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs	440:545	Advancement in tissue engineering provides an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs.
26454048	4	31	theme	hyaluronic	617:626	arg1	acid					628:631	hyaluronic acid	617:631	hyaluronic acid	617:631	This study reports a hydroxyapatite, calcium sulfate hemihydrate and hyaluronic acid laden collagenase (HAP/CS/HA-Col) as a bone substitute for the alveolar bone regeneration.
26454048	8	32	theme	bone	1202:1205	arg1	process					1218:1224	bone remodeling process	1202:1224	bone remodeling process	1202:1224	The collagenase release profile had facilitated the acceleration of bone remodeling process and it was confirmed by the findings of micro-CT and H&E staining.
26454048	7	33	theme	6.69 MPa	1047:1054	arg1	strength					1035:1042	The mechanical strength	1020:1042	The mechanical strength of 6.69 MPa with excellent biocompatibility	1020:1086	The mechanical strength of 6.69 MPa with excellent biocompatibility was obtained for the HAP/CS/HA-Col composite.
26454048	4	34	theme	alveolar	696:703	arg1	regeneration					710:721	the alveolar bone regeneration	692:721	the alveolar bone regeneration	692:721	This study reports a hydroxyapatite, calcium sulfate hemihydrate and hyaluronic acid laden collagenase (HAP/CS/HA-Col) as a bone substitute for the alveolar bone regeneration.
26454048	3	35	theme	tissue	412:417	arg1	engineering					419:429	tissue engineering	412:429	tissue engineering	412:429	Advancement in tissue engineering provides an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs.
26454048	8	36	theme	micro-CT	1266:1273	arg1	findings					1254:1261	the findings	1250:1261	the findings of micro-CT and H&E staining	1250:1290	The collagenase release profile had facilitated the acceleration of bone remodeling process and it was confirmed by the findings of micro-CT and H&E staining.
26454048	9	37	theme	containing	1345:1354	arg1	collagenase					1371:1381	HAP/CS/HA composite containing 2 mg/mL type I collagenase	1325:1381	HAP/CS/HA composite containing 2 mg/mL type I collagenase	1325:1381	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	1	38	theme	soft	243:246	arg1	tissue					248:253	the soft tissue	239:253	the soft tissue	239:253	Periodontitis is a very severe inflammatory condition of the periodontium that progressively damages the soft tissue and destroys the alveolar bone that supports the teeth.
26454048	10	39	theme	other	1755:1759	arg1	tissues					1771:1777	other defective tissues	1755:1777	other defective tissues	1755:1777	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	7	40	with	strength	1035:1042	arg1	biocompatibility					1071:1086	excellent biocompatibility	1061:1086	excellent biocompatibility	1061:1086	The mechanical strength of 6.69 MPa with excellent biocompatibility was obtained for the HAP/CS/HA-Col composite.
26454048	8	41	theme	process	1218:1224	arg1	acceleration					1186:1197	the acceleration	1182:1197	the acceleration of bone remodeling process	1182:1224	The collagenase release profile had facilitated the acceleration of bone remodeling process and it was confirmed by the findings of micro-CT and H&E staining.
26454048	6	42	theme	HAP/CS/HA-Col	933:945	arg1	composite					947:955	the HAP/CS/HA-Col composite	929:955	the HAP/CS/HA-Col composite	929:955	The in vivo bone formation was assessed in rat with alveolar bone defects and the bone augmentation by the HAP/CS/HA-Col composite was confirmed by micro-CT images and histological examination.
26454048	10	43	theme	defective	1761:1769	arg1	tissues					1771:1777	other defective tissues	1755:1777	other defective tissues	1755:1777	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	3	44	theme	dental	499:504	arg1	implants					506:513	suitable dental implants	490:513	suitable dental implants	490:513	Advancement in tissue engineering provides an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs.
26454048	10	45	theme	same	1720:1723	arg1	design					1725:1730	the same design	1716:1730	the same design	1716:1730	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	4	46	theme	sulfate	593:599	arg1	hemihydrate					601:611	calcium sulfate hemihydrate	585:611	calcium sulfate hemihydrate	585:611	This study reports a hydroxyapatite, calcium sulfate hemihydrate and hyaluronic acid laden collagenase (HAP/CS/HA-Col) as a bone substitute for the alveolar bone regeneration.
26454048	8	47	theme	remodeling	1207:1216	arg1	process					1218:1224	bone remodeling process	1202:1224	bone remodeling process	1202:1224	The collagenase release profile had facilitated the acceleration of bone remodeling process and it was confirmed by the findings of micro-CT and H&E staining.
26454048	9	48	theme	type	1364:1367	arg1	collagenase					1371:1381	HAP/CS/HA composite containing 2 mg/mL type I collagenase	1325:1381	HAP/CS/HA composite containing 2 mg/mL type I collagenase	1325:1381	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	6	49	dep	micro-CT	974:981	arg1	images					983:988	images	983:988	images	983:988	The in vivo bone formation was assessed in rat with alveolar bone defects and the bone augmentation by the HAP/CS/HA-Col composite was confirmed by micro-CT images and histological examination.
26454048	4	50	theme	bone	705:708	arg1	regeneration					710:721	the alveolar bone regeneration	692:721	the alveolar bone regeneration	692:721	This study reports a hydroxyapatite, calcium sulfate hemihydrate and hyaluronic acid laden collagenase (HAP/CS/HA-Col) as a bone substitute for the alveolar bone regeneration.
26454048	6	51	with	rat	869:871	arg1	defects					892:898	alveolar bone defects	878:898	alveolar bone defects	878:898	The in vivo bone formation was assessed in rat with alveolar bone defects and the bone augmentation by the HAP/CS/HA-Col composite was confirmed by micro-CT images and histological examination.
26454048	4	52	theme	calcium	585:591	arg1	hemihydrate					601:611	calcium sulfate hemihydrate	585:611	calcium sulfate hemihydrate	585:611	This study reports a hydroxyapatite, calcium sulfate hemihydrate and hyaluronic acid laden collagenase (HAP/CS/HA-Col) as a bone substitute for the alveolar bone regeneration.
26454048	9	53	theme	bone	1297:1300	arg1	defects					1302:1308	The bone defects	1293:1308	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase	1293:1381	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	3	54	from	Advancement	397:407	arg1	engineering					419:429	tissue engineering	412:429	tissue engineering	412:429	Advancement in tissue engineering provides an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs.
26454048	6	55	theme	bone	838:841	arg1	formation					843:851	The in vivo bone formation	826:851	The in vivo bone formation	826:851	The in vivo bone formation was assessed in rat with alveolar bone defects and the bone augmentation by the HAP/CS/HA-Col composite was confirmed by micro-CT images and histological examination.
26454048	3	56	theme	effective	443:451	arg1	regeneration					453:464	an effective regeneration	440:464	an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs	440:545	Advancement in tissue engineering provides an effective regeneration of osseous defects with suitable dental implants or tissue-engineered constructs.
26454048	10	57	theme	HAP/CS/HA	1634:1642	arg1	composite					1644:1652	HAP/CS/HA composite	1634:1652	HAP/CS/HA composite	1634:1652	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	6	58	theme	in vivo	830:836	arg1	formation					843:851	The in vivo bone formation	826:851	The in vivo bone formation	826:851	The in vivo bone formation was assessed in rat with alveolar bone defects and the bone augmentation by the HAP/CS/HA-Col composite was confirmed by micro-CT images and histological examination.
26454048	10	59	theme	bone	1694:1697	arg1	regeneration					1699:1710	the alveolar bone regeneration	1681:1710	the alveolar bone regeneration	1681:1710	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	9	60	theme	composite	1335:1343	arg1	collagenase					1371:1381	HAP/CS/HA composite containing 2 mg/mL type I collagenase	1325:1381	HAP/CS/HA composite containing 2 mg/mL type I collagenase	1325:1381	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	6	61	theme	bone	908:911	arg1	augmentation					913:924	the bone augmentation	904:924	the bone augmentation by the HAP/CS/HA-Col composite	904:955	The in vivo bone formation was assessed in rat with alveolar bone defects and the bone augmentation by the HAP/CS/HA-Col composite was confirmed by micro-CT images and histological examination.
26454048	7	62	theme	HAP/CS/HA-Col	1109:1121	arg1	composite					1123:1131	the HAP/CS/HA-Col composite	1105:1131	the HAP/CS/HA-Col composite	1105:1131	The mechanical strength of 6.69 MPa with excellent biocompatibility was obtained for the HAP/CS/HA-Col composite.
26454048	2	63	theme	limited	362:368	arg1	reparability					370:381	limited reparability	362:381	limited reparability of the teeth	362:394	The bone loss is naturally irreversible because of limited reparability of the teeth.
26454048	9	64	theme	collagenase	1509:1519	arg1	granules					1559:1566	the collagenase and the porous hydroxyapatite (p-HAP) granules	1505:1566	the collagenase and the porous hydroxyapatite (p-HAP) granules	1505:1566	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	4	65	dep	bone	672:675	arg1	substitute					677:686	substitute	677:686	substitute for the alveolar bone regeneration	677:721	This study reports a hydroxyapatite, calcium sulfate hemihydrate and hyaluronic acid laden collagenase (HAP/CS/HA-Col) as a bone substitute for the alveolar bone regeneration.
26454048	9	66	with	comparison	1454:1463	arg1	HAP/CS/HA					1474:1482	the HAP/CS/HA composite	1470:1492	the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules	1470:1566	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	1	67	theme	severe	162:167	arg1	condition					182:190	a very severe inflammatory condition	155:190	a very severe inflammatory condition of the periodontium that progressively damages the soft tissue and destroys the alveolar bone that supports the teeth	155:308	Periodontitis is a very severe inflammatory condition of the periodontium that progressively damages the soft tissue and destroys the alveolar bone that supports the teeth.
26454048	1	67	theme	severe	162:167	arg1	Periodontitis					138:150	Periodontitis	138:150	Periodontitis	138:150	Periodontitis is a very severe inflammatory condition of the periodontium that progressively damages the soft tissue and destroys the alveolar bone that supports the teeth.
26454048	9	68	theme	composite	1484:1492	arg1	HAP/CS/HA					1474:1482	the HAP/CS/HA composite	1470:1492	the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules	1470:1566	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	10	69	theme	alveolar	1685:1692	arg1	regeneration					1699:1710	the alveolar bone regeneration	1681:1710	the alveolar bone regeneration	1681:1710	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	9	70	theme	improved	1394:1401	arg1	formation					1412:1420	improved new bone formation	1394:1420	improved new bone formation with matured bone morphology	1394:1449	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	9	71	with	formation	1412:1420	arg1	morphology					1440:1449	matured bone morphology	1427:1449	matured bone morphology	1427:1449	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	1	72	theme	inflammatory	169:180	arg1	condition					182:190	a very severe inflammatory condition	155:190	a very severe inflammatory condition of the periodontium that progressively damages the soft tissue and destroys the alveolar bone that supports the teeth	155:308	Periodontitis is a very severe inflammatory condition of the periodontium that progressively damages the soft tissue and destroys the alveolar bone that supports the teeth.
26454048	1	72	theme	inflammatory	169:180	arg1	Periodontitis					138:150	Periodontitis	138:150	Periodontitis	138:150	Periodontitis is a very severe inflammatory condition of the periodontium that progressively damages the soft tissue and destroys the alveolar bone that supports the teeth.
26454048	1	73	theme	alveolar	272:279	arg1	bone					281:284	the alveolar bone	268:284	the alveolar bone that supports the teeth	268:308	Periodontitis is a very severe inflammatory condition of the periodontium that progressively damages the soft tissue and destroys the alveolar bone that supports the teeth.
26454048	9	74	theme	new	1403:1405	arg1	formation					1412:1420	improved new bone formation	1394:1420	improved new bone formation with matured bone morphology	1394:1449	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	0	75	theme	acid	42:45	arg1	composite					47:55	Hydroxyapatite-calcium sulfate-hyaluronic acid composite	0:55	Hydroxyapatite-calcium sulfate-hyaluronic acid composite encapsulated with collagenase as bone	0:93	Hydroxyapatite-calcium sulfate-hyaluronic acid composite encapsulated with collagenase as bone substitute for alveolar bone regeneration.
26454048	10	76	from	inclusion	1621:1629	arg1	composite					1644:1652	HAP/CS/HA composite	1634:1652	HAP/CS/HA composite	1634:1652	The said findings demonstrated that the collagenase inclusion in HAP/CS/HA composite is a feasible approach for the alveolar bone regeneration and the same design can also be applied to other defective tissues.
26454048	9	77	theme	porous	1529:1534	arg1	p-HAP					1552:1556	p-HAP	1552:1556	p-HAP	1552:1556	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	9	77	theme	porous	1529:1534	arg1	hydroxyapatite					1536:1549	the porous hydroxyapatite	1525:1549	the porous hydroxyapatite (p-HAP)	1525:1557	The bone defects implanted with HAP/CS/HA composite containing 2 mg/mL type I collagenase have shown improved new bone formation with matured bone morphology in comparison with the HAP/CS/HA composite that lacks the collagenase and the porous hydroxyapatite (p-HAP) granules.
26454048	5	78	theme	WST-1	813:817	arg1	assay					819:823	WST-1 assay	813:823	WST-1 assay	813:823	The composite material was mechanically tested and the biocompatibility was evaluated by WST-1 assay.
24907571	1	0	theme	products	360:367	arg1	composition					335:345	the composition	331:345	the composition of generated products	331:367	As the exploration of the carbonization of mixed feedstocks continues, there is a distinct need to understand how feedstock chemical composition and structural complexity influence the composition of generated products.
24907571	4	1	dep	solids	935:940	arg1	groups					961:966	surface functional groups	942:966	solids surface functional groups	935:966	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	4	2	from	carbonization	731:743	arg1	results					714:720	results	714:720	results from the carbonization of the pure compounds	714:765	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	0	3	theme	hydrothermal	102:113	arg1	carbonization					115:127	the hydrothermal carbonization	98:127	the hydrothermal carbonization of mixed feedstocks	98:147	Influence of feedstock chemical composition on product formation and characteristics derived from the hydrothermal carbonization of mixed feedstocks.
24907571	0	4	from	Influence	0:8	arg1	characteristics					69:83	characteristics	69:83	characteristics	69:83	Influence of feedstock chemical composition on product formation and characteristics derived from the hydrothermal carbonization of mixed feedstocks.
24907571	0	4	from	Influence	0:8	arg1	formation					55:63	product formation	47:63	product formation	47:63	Influence of feedstock chemical composition on product formation and characteristics derived from the hydrothermal carbonization of mixed feedstocks.
24907571	1	5	theme	feedstock	264:272	arg1	composition					283:293	feedstock chemical composition	264:293	feedstock chemical composition	264:293	As the exploration of the carbonization of mixed feedstocks continues, there is a distinct need to understand how feedstock chemical composition and structural complexity influence the composition of generated products.
24907571	2	6	dep	paper	551:555	arg1	e.g.					545:548	e.g.	545:548	e.g.	545:548	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	5	7	theme	feedstock	1150:1158	arg1	properties					1160:1169	additional feedstock properties	1139:1169	additional feedstock properties	1139:1169	To more accurately predict carbonization products, it may be necessary to account for feedstock structure and/or additional feedstock properties.
24907571	1	8	theme	chemical	274:281	arg1	composition					283:293	feedstock chemical composition	264:293	feedstock chemical composition	264:293	As the exploration of the carbonization of mixed feedstocks continues, there is a distinct need to understand how feedstock chemical composition and structural complexity influence the composition of generated products.
24907571	4	9	theme	linear	1009:1014	arg1	approach					1016:1023	this linear approach	1004:1023	this linear approach	1004:1023	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	4	10	theme	compounds	757:765	arg1	carbonization					731:743	the carbonization	727:743	the carbonization of the pure compounds	727:765	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	1	11	theme	carbonization	176:188	arg1	exploration					157:167	the exploration	153:167	the exploration of the carbonization of mixed feedstocks	153:208	As the exploration of the carbonization of mixed feedstocks continues, there is a distinct need to understand how feedstock chemical composition and structural complexity influence the composition of generated products.
24907571	2	12	theme	compounds	478:486	arg1	compounds					446:454	pure compounds	441:454	pure compounds	441:454	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	2	12	theme	compounds	478:486	arg1	feedstocks					501:510	complex feedstocks	493:510	complex feedstocks comprised of the pure compounds (e.g., paper, wood)	493:562	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	2	12	theme	compounds	478:486	arg1	mixtures					457:464	mixtures	457:464	mixtures of the pure compounds	457:486	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	4	13	theme	recovered	785:793	arg1	contents					809:816	recovered solids energy contents	785:816	recovered solids energy contents	785:816	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	1	14	theme	mixed	193:197	arg1	feedstocks					199:208	mixed feedstocks	193:208	mixed feedstocks	193:208	As the exploration of the carbonization of mixed feedstocks continues, there is a distinct need to understand how feedstock chemical composition and structural complexity influence the composition of generated products.
24907571	2	15	theme	pure	473:476	arg1	compounds					478:486	the pure compounds	469:486	the pure compounds	469:486	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	1	16	theme	feedstocks	199:208	arg1	carbonization					176:188	the carbonization	172:188	the carbonization of mixed feedstocks	172:208	As the exploration of the carbonization of mixed feedstocks continues, there is a distinct need to understand how feedstock chemical composition and structural complexity influence the composition of generated products.
24907571	0	17	theme	chemical	23:30	arg1	composition					32:42	feedstock chemical composition	13:42	feedstock chemical composition	13:42	Influence of feedstock chemical composition on product formation and characteristics derived from the hydrothermal carbonization of mixed feedstocks.
24907571	5	18	theme	carbonization	1053:1065	arg1	products					1067:1074	carbonization products	1053:1074	carbonization products	1053:1074	To more accurately predict carbonization products, it may be necessary to account for feedstock structure and/or additional feedstock properties.
24907571	0	19	theme	feedstocks	138:147	arg1	carbonization					115:127	the hydrothermal carbonization	98:127	the hydrothermal carbonization of mixed feedstocks	98:147	Influence of feedstock chemical composition on product formation and characteristics derived from the hydrothermal carbonization of mixed feedstocks.
24907571	4	20	theme	energy	802:807	arg1	contents					809:816	recovered solids energy contents	785:816	recovered solids energy contents	785:816	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	2	21	theme	mixtures	457:464	arg1	carbonization					424:436	the carbonization	420:436	the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood)	420:562	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	0	22	theme	feedstock	13:21	arg1	composition					32:42	feedstock chemical composition	13:42	feedstock chemical composition	13:42	Influence of feedstock chemical composition on product formation and characteristics derived from the hydrothermal carbonization of mixed feedstocks.
24907571	1	23	theme	structural	299:308	arg1	complexity					310:319	structural complexity	299:319	structural complexity	299:319	As the exploration of the carbonization of mixed feedstocks continues, there is a distinct need to understand how feedstock chemical composition and structural complexity influence the composition of generated products.
24907571	0	24	theme	mixed	132:136	arg1	feedstocks					138:147	mixed feedstocks	132:147	mixed feedstocks	132:147	Influence of feedstock chemical composition on product formation and characteristics derived from the hydrothermal carbonization of mixed feedstocks.
24907571	5	25	theme	feedstock	1112:1120	arg1	structure					1122:1130	feedstock structure	1112:1130	feedstock structure	1112:1130	To more accurately predict carbonization products, it may be necessary to account for feedstock structure and/or additional feedstock properties.
24907571	4	26	theme	solids	795:800	arg1	contents					809:816	recovered solids energy contents	785:816	recovered solids energy contents	785:816	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	4	27	theme	Carbonization	655:667	arg1	characteristics					677:691	Carbonization product characteristics	655:691	Carbonization product characteristics	655:691	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	2	28	theme	feedstocks	501:510	arg1	carbonization					424:436	the carbonization	420:436	the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood)	420:562	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	2	29	theme	compounds	446:454	arg1	carbonization					424:436	the carbonization	420:436	the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood)	420:562	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	0	30	theme	composition	32:42	arg1	Influence					0:8	Influence	0:8	Influence of feedstock chemical composition on product formation and characteristics	0:83	Influence of feedstock chemical composition on product formation and characteristics derived from the hydrothermal carbonization of mixed feedstocks.
24907571	2	31	theme	complex	493:499	arg1	feedstocks					501:510	complex feedstocks	493:510	complex feedstocks comprised of the pure compounds (e.g., paper, wood)	493:562	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	4	32	from	mass	881:884	arg1	phase					894:898	each phase	889:898	each phase	889:898	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	2	33	theme	pure	441:444	arg1	compounds					446:454	pure compounds	441:454	pure compounds	441:454	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	4	34	theme	surface	942:948	arg1	groups					961:966	surface functional groups	942:966	solids surface functional groups	935:966	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	5	35	theme	additional	1139:1148	arg1	properties					1160:1169	additional feedstock properties	1139:1169	additional feedstock properties	1139:1169	To more accurately predict carbonization products, it may be necessary to account for feedstock structure and/or additional feedstock properties.
24907571	3	36	theme	feedstock	587:595	arg1	properties					597:606	feedstock properties	587:606	feedstock properties	587:606	Results indicate that feedstock properties do influence carbonization product properties.
24907571	2	37	theme	Laboratory	370:379	arg1	experiments					381:391	Laboratory experiments	370:391	Laboratory experiments	370:391	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	0	38	theme	product	47:53	arg1	formation					55:63	product formation	47:63	product formation	47:63	Influence of feedstock chemical composition on product formation and characteristics derived from the hydrothermal carbonization of mixed feedstocks.
24907571	4	39	theme	carbon	874:879	arg1	mass					881:884	the carbon mass	870:884	the carbon mass	870:884	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	4	40	theme	pure	752:755	arg1	compounds					757:765	the pure compounds	748:765	the pure compounds	748:765	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	4	41	theme	solid	853:857	arg1	yields					859:864	solid yields	853:864	solid yields	853:864	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	4	42	theme	functional	950:959	arg1	groups					961:966	surface functional groups	942:966	solids surface functional groups	935:966	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	4	43	from	yields	859:864	arg1	phase					894:898	each phase	889:898	each phase	889:898	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	1	44	theme	distinct	232:239	arg1	need					241:244	a distinct need	230:244	a distinct need to understand how feedstock chemical composition and structural complexity influence the composition of generated products	230:367	As the exploration of the carbonization of mixed feedstocks continues, there is a distinct need to understand how feedstock chemical composition and structural complexity influence the composition of generated products.
24907571	2	45	theme	pure	529:532	arg1	compounds					534:542	the pure compounds	525:542	the pure compounds (e.g., paper, wood)	525:562	Laboratory experiments were conducted to evaluate the carbonization of pure compounds, mixtures of the pure compounds, and complex feedstocks comprised of the pure compounds (e.g., paper, wood).
24907571	3	46	theme	carbonization	621:633	arg1	properties					643:652	carbonization product properties	621:652	carbonization product properties	621:652	Results indicate that feedstock properties do influence carbonization product properties.
24907571	4	47	theme	product	669:675	arg1	characteristics					677:691	Carbonization product characteristics	655:691	Carbonization product characteristics	655:691	Carbonization product characteristics were predicted using results from the carbonization of the pure compounds and indicate that recovered solids energy contents are more accurately predicted than solid yields and the carbon mass in each phase, while predictions associated with solids surface functional groups are more difficult to predict using this linear approach.
24907571	1	48	theme	generated	350:358	arg1	products					360:367	generated products	350:367	generated products	350:367	As the exploration of the carbonization of mixed feedstocks continues, there is a distinct need to understand how feedstock chemical composition and structural complexity influence the composition of generated products.
24907571	3	49	theme	product	635:641	arg1	properties					643:652	carbonization product properties	621:652	carbonization product properties	621:652	Results indicate that feedstock properties do influence carbonization product properties.
29214219	2	0	link	bacteria-derived	326:341	arg1	materials					354:362	bacteria-derived functional materials	326:362	bacteria-derived functional materials	326:362	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	1	1	theme	embedded	158:165	arg1	bacteria					149:156	bacteria	149:156	bacteria embedded in materials	149:178	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	1	2	from	embedded	158:165	arg1	materials					170:178	materials	170:178	materials	170:178	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	3	3	theme	biocompatible	520:532	arg1	ink					565:567	a biocompatible and functionalized 3D printing ink	518:567	a biocompatible and functionalized 3D printing ink	518:567	To achieve this, we embedded bacteria in a biocompatible and functionalized 3D printing ink and printed two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose.
29214219	1	4	theme	major	261:265	arg1	challenge					267:275	a major challenge	259:275	a major challenge	259:275	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	2	5	theme	3D	295:296	arg1	approach					307:314	a 3D printing approach	293:314	a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing	293:474	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	2	6	theme	printing	298:305	arg1	approach					307:314	a 3D printing approach	293:314	a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing	293:474	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	4	7	theme	bacteria-printing	722:738	arg1	platform					740:747	this versatile bacteria-printing platform	707:747	this versatile bacteria-printing platform	707:747	With this versatile bacteria-printing platform, complex materials displaying spatially specific compositions, geometry, and properties not accessed by standard technologies can be assembled from bottom up for new biotechnological and biomedical applications.
29214219	2	8	theme	bacteria-derived	326:341	arg1	materials					354:362	bacteria-derived functional materials	326:362	bacteria-derived functional materials	326:362	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	4	9	theme	versatile	712:720	arg1	platform					740:747	this versatile bacteria-printing platform	707:747	this versatile bacteria-printing platform	707:747	With this versatile bacteria-printing platform, complex materials displaying spatially specific compositions, geometry, and properties not accessed by standard technologies can be assembled from bottom up for new biotechnological and biomedical applications.
29214219	2	10	theme	design	435:440	arg1	freedom					442:448	the shape design freedom	425:448	the shape design freedom of additive manufacturing	425:474	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	1	11	theme	recent	67:72	arg1	advances					74:81	recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials	67:178	recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials	67:178	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	2	12	theme	manufacturing	462:474	arg1	freedom					442:448	the shape design freedom	425:448	the shape design freedom of additive manufacturing	425:474	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	2	13	theme	bacteria	411:418	arg1	metabolism					397:406	the natural diverse metabolism	377:406	the natural diverse metabolism of bacteria	377:418	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	3	14	theme	functionalized	538:551	arg1	ink					565:567	a biocompatible and functionalized 3D printing ink	518:567	a biocompatible and functionalized 3D printing ink	518:567	To achieve this, we embedded bacteria in a biocompatible and functionalized 3D printing ink and printed two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose.
29214219	1	15	theme	bacterial	181:189	arg1	localization					191:202	bacterial localization	181:202	bacterial localization into complex three-dimensional (3D) geometries	181:249	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	0	16	theme	3D	0:1	arg1	printing					3:10	3D printing	0:10	3D printing of bacteria into functional complex materials.	0:57	3D printing of bacteria into functional complex materials.
29214219	4	17	theme	bottom	897:902	arg1	up					904:905	bottom up	897:905	bottom up for new biotechnological and biomedical applications	897:958	With this versatile bacteria-printing platform, complex materials displaying spatially specific compositions, geometry, and properties not accessed by standard technologies can be assembled from bottom up for new biotechnological and biomedical applications.
29214219	3	18	from	bacteria	506:513	arg1	ink					565:567	a biocompatible and functionalized 3D printing ink	518:567	a biocompatible and functionalized 3D printing ink	518:567	To achieve this, we embedded bacteria in a biocompatible and functionalized 3D printing ink and printed two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose.
29214219	0	19	theme	bacteria	15:22	arg1	printing					3:10	3D printing	0:10	3D printing of bacteria into functional complex materials.	0:57	3D printing of bacteria into functional complex materials.
29214219	4	20	theme	complex	750:756	arg1	materials					758:766	complex materials	750:766	complex materials displaying spatially specific compositions, geometry, and properties not accessed by standard technologies	750:873	With this versatile bacteria-printing platform, complex materials displaying spatially specific compositions, geometry, and properties not accessed by standard technologies can be assembled from bottom up for new biotechnological and biomedical applications.
29214219	3	21	theme	3D	553:554	arg1	ink					565:567	a biocompatible and functionalized 3D printing ink	518:567	a biocompatible and functionalized 3D printing ink	518:567	To achieve this, we embedded bacteria in a biocompatible and functionalized 3D printing ink and printed two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose.
29214219	3	22	theme	producing	652:660	arg1	types					585:589	two types	581:589	two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose	581:699	To achieve this, we embedded bacteria in a biocompatible and functionalized 3D printing ink and printed two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose.
29214219	1	23	theme	spatial	98:104	arg1	composition					106:116	the spatial composition	94:116	the spatial composition	94:116	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	1	24	theme	complex	209:215	arg1	geometries					240:249	complex three-dimensional (3D) geometries	209:249	complex three-dimensional (3D) geometries	209:249	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	0	25	theme	functional	29:38	arg1	materials					48:56	functional complex materials	29:56	functional complex materials	29:56	3D printing of bacteria into functional complex materials.
29214219	1	26	theme	three-dimensional	217:233	arg1	geometries					240:249	complex three-dimensional (3D) geometries	209:249	complex three-dimensional (3D) geometries	209:249	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	3	27	theme	materials	602:610	arg1	types					585:589	two types	581:589	two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose	581:699	To achieve this, we embedded bacteria in a biocompatible and functionalized 3D printing ink and printed two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose.
29214219	2	28	theme	shape	429:433	arg1	freedom					442:448	the shape design freedom	425:448	the shape design freedom of additive manufacturing	425:474	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	2	29	theme	additive	453:460	arg1	manufacturing					462:474	additive manufacturing	453:474	additive manufacturing	453:474	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	0	30	theme	complex	40:46	arg1	materials					48:56	functional complex materials	29:56	functional complex materials	29:56	3D printing of bacteria into functional complex materials.
29214219	4	31	theme	biomedical	936:945	arg1	applications					947:958	new biotechnological and biomedical applications	911:958	applications	947:958	With this versatile bacteria-printing platform, complex materials displaying spatially specific compositions, geometry, and properties not accessed by standard technologies can be assembled from bottom up for new biotechnological and biomedical applications.
29214219	3	32	theme	living	595:600	arg1	materials					602:610	"living materials	594:610	"living materials" capable of degrading pollutants	594:643	To achieve this, we embedded bacteria in a biocompatible and functionalized 3D printing ink and printed two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose.
29214219	1	33	dep	three-dimensional	217:233	arg1	3D					236:237	3D	236:237	3D	236:237	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	4	34	theme	standard	853:860	arg1	technologies					862:873	standard technologies	853:873	standard technologies	853:873	With this versatile bacteria-printing platform, complex materials displaying spatially specific compositions, geometry, and properties not accessed by standard technologies can be assembled from bottom up for new biotechnological and biomedical applications.
29214219	1	35	theme	dynamic	122:128	arg1	functionalities					130:144	dynamic functionalities	122:144	dynamic functionalities	122:144	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	3	36	theme	printing	556:563	arg1	ink					565:567	a biocompatible and functionalized 3D printing ink	518:567	a biocompatible and functionalized 3D printing ink	518:567	To achieve this, we embedded bacteria in a biocompatible and functionalized 3D printing ink and printed two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose.
29214219	3	37	theme	relevant	672:679	arg1	cellulose					691:699	medically relevant bacterial cellulose	662:699	medically relevant bacterial cellulose	662:699	To achieve this, we embedded bacteria in a biocompatible and functionalized 3D printing ink and printed two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose.
29214219	4	38	theme	specific	789:796	arg1	compositions					798:809	spatially specific compositions	779:809	spatially specific compositions	779:809	With this versatile bacteria-printing platform, complex materials displaying spatially specific compositions, geometry, and properties not accessed by standard technologies can be assembled from bottom up for new biotechnological and biomedical applications.
29214219	2	39	theme	natural	381:387	arg1	metabolism					397:406	the natural diverse metabolism	377:406	the natural diverse metabolism of bacteria	377:418	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	2	40	theme	functional	343:352	arg1	materials					354:362	bacteria-derived functional materials	326:362	bacteria-derived functional materials	326:362	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	3	41	theme	bacterial	681:689	arg1	cellulose					691:699	medically relevant bacterial cellulose	662:699	medically relevant bacterial cellulose	662:699	To achieve this, we embedded bacteria in a biocompatible and functionalized 3D printing ink and printed two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose.
29214219	3	42	theme	capable	613:619	arg1	materials					602:610	"living materials	594:610	"living materials" capable of degrading pollutants	594:643	To achieve this, we embedded bacteria in a biocompatible and functionalized 3D printing ink and printed two types of "living materials" capable of degrading pollutants and of producing medically relevant bacterial cellulose.
29214219	2	43	theme	diverse	389:395	arg1	metabolism					397:406	the natural diverse metabolism	377:406	the natural diverse metabolism of bacteria	377:418	We demonstrate a 3D printing approach to create bacteria-derived functional materials by combining the natural diverse metabolism of bacteria with the shape design freedom of additive manufacturing.
29214219	1	44	from	materials	170:178	arg1	embedded					158:165	embedded	158:165	embedded	158:165	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	1	45	theme	bacteria	149:156	arg1	composition					106:116	the spatial composition	94:116	the spatial composition	94:116	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
29214219	1	45	theme	bacteria	149:156	arg1	functionalities					130:144	dynamic functionalities	122:144	dynamic functionalities	122:144	Despite recent advances to control the spatial composition and dynamic functionalities of bacteria embedded in materials, bacterial localization into complex three-dimensional (3D) geometries remains a major challenge.
27979129	3	0	from	properties	383:392	arg1	samples					429:435	7 quinoa samples	420:435	7 quinoa samples	420:435	In this study, composition and physicochemical properties of whole grain flour from 7 quinoa samples have been analyzed.
27979129	7	1	with	Interactions	900:911	arg1	minerals					1008:1015	minerals	1008:1015	minerals	1008:1015	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	7	1	with	Interactions	900:911	arg1	components					939:948	non-starch components	928:948	non-starch components	928:948	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	7	1	with	Interactions	900:911	arg1	fibre					986:990	fibre	986:990	fibre	986:990	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	7	1	with	Interactions	900:911	arg1	lipids					961:966	lipids	961:966	lipids	961:966	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	7	1	with	Interactions	900:911	arg1	phenolics					993:1001	phenolics	993:1001	phenolics	993:1001	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	7	1	with	Interactions	900:911	arg1	protein					969:975	protein	969:975	protein	969:975	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	1	2	theme	new	143:145	arg1	development					155:165	new product development	143:165	new product development due to the unique nutritional benefits	143:204	There has been growing interest in whole grain quinoa flour for new product development due to the unique nutritional benefits.
27979129	4	3	theme	starches	558:565	arg1	composition					508:518	flour composition	502:518	flour composition	502:518	Flour properties have been correlated to the flour composition and the properties of isolated quinoa starches through chemometrics.
27979129	4	3	theme	starches	558:565	arg1	properties					528:537	the properties	524:537	the properties	524:537	Flour properties have been correlated to the flour composition and the properties of isolated quinoa starches through chemometrics.
27979129	4	4	dep	composition	508:518	arg1	the					498:500	the	498:500	the	498:500	Flour properties have been correlated to the flour composition and the properties of isolated quinoa starches through chemometrics.
27979129	9	5	theme	crop	1406:1409	arg1	utilization					1376:1386	better utilization	1369:1386	better utilization of this specialty crop	1369:1409	Understanding the roles of starch and other components in physicochemical properties of quinoa flour provides a basis for better utilization of this specialty crop.
27979129	2	6	theme	components	324:333	arg1	properties					261:270	the properties	257:270	the properties of its major component starch as well as non-starch components	257:333	The quality of quinoa flour is much determined by the properties of its major component starch as well as non-starch components.
27979129	4	7	theme	quinoa	551:556	arg1	starches					558:565	isolated quinoa starches	542:565	isolated quinoa starches	542:565	Flour properties have been correlated to the flour composition and the properties of isolated quinoa starches through chemometrics.
27979129	5	8	theme	water	647:651	arg1	index					661:665	water soluble index	647:665	water soluble index	647:665	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	9	theme	Great	589:593	arg1	variations					595:604	Great variations	589:604	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour	589:747	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	9	10	theme	better	1369:1374	arg1	utilization					1376:1386	better utilization	1369:1386	better utilization of this specialty crop	1369:1409	Understanding the roles of starch and other components in physicochemical properties of quinoa flour provides a basis for better utilization of this specialty crop.
27979129	5	11	theme	soluble	653:659	arg1	index					661:665	water soluble index	647:665	water soluble index	647:665	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	12	theme	flour	743:747	arg1	pasting					691:697	pasting	691:697	pasting	691:697	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	12	theme	flour	743:747	arg1	composition					618:628	chemical composition	609:628	chemical composition	609:628	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	12	theme	flour	743:747	arg1	properties					725:734	thermal properties	717:734	thermal properties of the flour	717:747	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	12	theme	flour	743:747	arg1	index					661:665	water soluble index	647:665	water soluble index	647:665	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	12	theme	flour	743:747	arg1	texture					704:710	gel texture	700:710	gel texture	700:710	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	12	theme	flour	743:747	arg1	susceptibility					675:688	enzyme susceptibility	668:688	enzyme susceptibility	668:688	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	12	theme	flour	743:747	arg1	power					640:644	swelling power	631:644	swelling power	631:644	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	13	theme	chemical	609:616	arg1	composition					618:628	chemical composition	609:628	chemical composition	609:628	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	6	14	theme	Correlation	769:779	arg1	analysis					781:788	Correlation analysis	769:788	Correlation analysis	769:788	Correlation analysis showed that thermal properties and enzyme susceptibility of quinoa flour are highly influenced by the starch.
27979129	8	15	dep	content	1219:1225	arg1	p<0.01					1238:1243	p<0.01	1238:1243	p<0.01	1238:1243	For example, peak gelatinization temperature of the flour is positively correlated to that of the starch (r=0.948, p<0.01) and negatively correlated to the lipid content (r=-0.951, p<0.01).
27979129	8	15	dep	content	1219:1225	arg1	r=-0.951					1228:1235	r=-0.951	1228:1235	r=-0.951	1228:1235	For example, peak gelatinization temperature of the flour is positively correlated to that of the starch (r=0.948, p<0.01) and negatively correlated to the lipid content (r=-0.951, p<0.01).
27979129	9	16	theme	specialty	1396:1404	arg1	crop					1406:1409	this specialty crop	1391:1409	this specialty crop	1391:1409	Understanding the roles of starch and other components in physicochemical properties of quinoa flour provides a basis for better utilization of this specialty crop.
27979129	5	17	theme	thermal	717:723	arg1	properties					725:734	thermal properties	717:734	thermal properties of the flour	717:747	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	3	18	from	composition	351:361	arg1	samples					429:435	7 quinoa samples	420:435	7 quinoa samples	420:435	In this study, composition and physicochemical properties of whole grain flour from 7 quinoa samples have been analyzed.
27979129	1	19	theme	due	167:169	arg1	development					155:165	new product development	143:165	new product development due to the unique nutritional benefits	143:204	There has been growing interest in whole grain quinoa flour for new product development due to the unique nutritional benefits.
27979129	9	20	from	roles	1265:1269	arg1	properties					1321:1330	physicochemical properties	1305:1330	physicochemical properties of quinoa flour	1305:1346	Understanding the roles of starch and other components in physicochemical properties of quinoa flour provides a basis for better utilization of this specialty crop.
27979129	4	21	theme	Flour	457:461	arg1	properties					463:472	Flour properties	457:472	Flour properties	457:472	Flour properties have been correlated to the flour composition and the properties of isolated quinoa starches through chemometrics.
27979129	7	22	theme	non-starch	928:937	arg1	minerals					1008:1015	minerals	1008:1015	minerals	1008:1015	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	7	22	theme	non-starch	928:937	arg1	components					939:948	non-starch components	928:948	non-starch components	928:948	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	7	22	theme	non-starch	928:937	arg1	fibre					986:990	fibre	986:990	fibre	986:990	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	7	22	theme	non-starch	928:937	arg1	lipids					961:966	lipids	961:966	lipids	961:966	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	7	22	theme	non-starch	928:937	arg1	phenolics					993:1001	phenolics	993:1001	phenolics	993:1001	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	7	22	theme	non-starch	928:937	arg1	protein					969:975	protein	969:975	protein	969:975	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	2	23	theme	starch	295:300	arg1	properties					261:270	the properties	257:270	the properties of its major component starch as well as non-starch components	257:333	The quality of quinoa flour is much determined by the properties of its major component starch as well as non-starch components.
27979129	3	24	theme	grain	403:407	arg1	flour					409:413	whole grain flour	397:413	whole grain flour from 7 quinoa samples	397:435	In this study, composition and physicochemical properties of whole grain flour from 7 quinoa samples have been analyzed.
27979129	0	25	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of quinoa flour	0:41	Physicochemical properties of quinoa flour as affected by starch interactions.
27979129	9	26	theme	starch	1274:1279	arg1	roles					1265:1269	the roles	1261:1269	the roles of starch and other components in physicochemical properties of quinoa flour	1261:1346	Understanding the roles of starch and other components in physicochemical properties of quinoa flour provides a basis for better utilization of this specialty crop.
27979129	5	27	theme	swelling	631:638	arg1	power					640:644	swelling power	631:644	swelling power	631:644	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	2	28	theme	component	285:293	arg1	starch					295:300	its major component starch	275:300	its major component starch as well as non-starch components	275:333	The quality of quinoa flour is much determined by the properties of its major component starch as well as non-starch components.
27979129	8	29	theme	lipid	1213:1217	arg1	content					1219:1225	the lipid content	1209:1225	the lipid content (r=-0.951, p<0.01)	1209:1244	For example, peak gelatinization temperature of the flour is positively correlated to that of the starch (r=0.948, p<0.01) and negatively correlated to the lipid content (r=-0.951, p<0.01).
27979129	3	30	theme	flour	409:413	arg1	composition					351:361	composition	351:361	composition	351:361	In this study, composition and physicochemical properties of whole grain flour from 7 quinoa samples have been analyzed.
27979129	3	30	theme	flour	409:413	arg1	properties					383:392	physicochemical properties	367:392	physicochemical properties of whole grain flour from 7 quinoa samples	367:435	In this study, composition and physicochemical properties of whole grain flour from 7 quinoa samples have been analyzed.
27979129	0	31	theme	quinoa	30:35	arg1	flour					37:41	quinoa flour	30:41	quinoa flour	30:41	Physicochemical properties of quinoa flour as affected by starch interactions.
27979129	7	32	theme	flour	1039:1043	arg1	properties					1045:1054	the flour properties	1035:1054	the flour properties	1035:1054	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	7	33	theme	starch	916:921	arg1	Interactions					900:911	Interactions	900:911	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals,	900:1016	Interactions of starch with non-starch components, including lipids, protein, dietary fibre, phenolics, and minerals, greatly impacted the flour properties.
27979129	2	34	theme	major	279:283	arg1	starch					295:300	its major component starch	275:300	its major component starch as well as non-starch components	275:333	The quality of quinoa flour is much determined by the properties of its major component starch as well as non-starch components.
27979129	1	35	theme	unique	178:183	arg1	benefits					197:204	the unique nutritional benefits	174:204	the unique nutritional benefits	174:204	There has been growing interest in whole grain quinoa flour for new product development due to the unique nutritional benefits.
27979129	4	36	theme	isolated	542:549	arg1	starches					558:565	isolated quinoa starches	542:565	isolated quinoa starches	542:565	Flour properties have been correlated to the flour composition and the properties of isolated quinoa starches through chemometrics.
27979129	6	37	theme	flour	857:861	arg1	properties					810:819	thermal properties	802:819	thermal properties	802:819	Correlation analysis showed that thermal properties and enzyme susceptibility of quinoa flour are highly influenced by the starch.
27979129	6	37	theme	flour	857:861	arg1	susceptibility					832:845	enzyme susceptibility	825:845	enzyme susceptibility	825:845	Correlation analysis showed that thermal properties and enzyme susceptibility of quinoa flour are highly influenced by the starch.
27979129	1	38	theme	product	147:153	arg1	development					155:165	new product development	143:165	new product development due to the unique nutritional benefits	143:204	There has been growing interest in whole grain quinoa flour for new product development due to the unique nutritional benefits.
27979129	1	39	theme	nutritional	185:195	arg1	benefits					197:204	the unique nutritional benefits	174:204	the unique nutritional benefits	174:204	There has been growing interest in whole grain quinoa flour for new product development due to the unique nutritional benefits.
27979129	9	40	theme	components	1291:1300	arg1	roles					1265:1269	the roles	1261:1269	the roles of starch and other components in physicochemical properties of quinoa flour	1261:1346	Understanding the roles of starch and other components in physicochemical properties of quinoa flour provides a basis for better utilization of this specialty crop.
27979129	6	41	theme	quinoa	850:855	arg1	flour					857:861	quinoa flour	850:861	quinoa flour	850:861	Correlation analysis showed that thermal properties and enzyme susceptibility of quinoa flour are highly influenced by the starch.
27979129	2	42	theme	quinoa	222:227	arg1	flour					229:233	quinoa flour	222:233	quinoa flour	222:233	The quality of quinoa flour is much determined by the properties of its major component starch as well as non-starch components.
27979129	1	43	theme	growing	94:100	arg1	interest					102:109	growing interest	94:109	growing interest in whole grain quinoa flour	94:137	There has been growing interest in whole grain quinoa flour for new product development due to the unique nutritional benefits.
27979129	3	44	theme	quinoa	422:427	arg1	samples					429:435	7 quinoa samples	420:435	7 quinoa samples	420:435	In this study, composition and physicochemical properties of whole grain flour from 7 quinoa samples have been analyzed.
27979129	0	45	theme	flour	37:41	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of quinoa flour	0:41	Physicochemical properties of quinoa flour as affected by starch interactions.
27979129	4	46	theme	flour	502:506	arg1	composition					508:518	flour composition	502:518	flour composition	502:518	Flour properties have been correlated to the flour composition and the properties of isolated quinoa starches through chemometrics.
27979129	3	47	theme	whole	397:401	arg1	flour					409:413	whole grain flour	397:413	whole grain flour from 7 quinoa samples	397:435	In this study, composition and physicochemical properties of whole grain flour from 7 quinoa samples have been analyzed.
27979129	3	48	theme	physicochemical	367:381	arg1	properties					383:392	physicochemical properties	367:392	physicochemical properties of whole grain flour from 7 quinoa samples	367:435	In this study, composition and physicochemical properties of whole grain flour from 7 quinoa samples have been analyzed.
27979129	9	49	theme	physicochemical	1305:1319	arg1	properties					1321:1330	physicochemical properties	1305:1330	physicochemical properties of quinoa flour	1305:1346	Understanding the roles of starch and other components in physicochemical properties of quinoa flour provides a basis for better utilization of this specialty crop.
27979129	8	50	theme	gelatinization	1075:1088	arg1	temperature					1090:1100	peak gelatinization temperature	1070:1100	peak gelatinization temperature of the flour	1070:1113	For example, peak gelatinization temperature of the flour is positively correlated to that of the starch (r=0.948, p<0.01) and negatively correlated to the lipid content (r=-0.951, p<0.01).
27979129	3	51	from	samples	429:435	arg1	composition					351:361	composition	351:361	composition	351:361	In this study, composition and physicochemical properties of whole grain flour from 7 quinoa samples have been analyzed.
27979129	3	51	from	samples	429:435	arg1	properties					383:392	physicochemical properties	367:392	physicochemical properties of whole grain flour from 7 quinoa samples	367:435	In this study, composition and physicochemical properties of whole grain flour from 7 quinoa samples have been analyzed.
27979129	3	51	from	samples	429:435	arg1	flour					409:413	whole grain flour	397:413	whole grain flour from 7 quinoa samples	397:435	In this study, composition and physicochemical properties of whole grain flour from 7 quinoa samples have been analyzed.
27979129	2	52	theme	non-starch	313:322	arg1	components					324:333	non-starch components	313:333	its major component starch as well as non-starch components	275:333	The quality of quinoa flour is much determined by the properties of its major component starch as well as non-starch components.
27979129	5	53	theme	gel	700:702	arg1	texture					704:710	gel texture	700:710	gel texture	700:710	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	8	54	theme	peak	1070:1073	arg1	temperature					1090:1100	peak gelatinization temperature	1070:1100	peak gelatinization temperature of the flour	1070:1113	For example, peak gelatinization temperature of the flour is positively correlated to that of the starch (r=0.948, p<0.01) and negatively correlated to the lipid content (r=-0.951, p<0.01).
27979129	6	55	theme	enzyme	825:830	arg1	susceptibility					832:845	enzyme susceptibility	825:845	enzyme susceptibility	825:845	Correlation analysis showed that thermal properties and enzyme susceptibility of quinoa flour are highly influenced by the starch.
27979129	1	56	theme	whole	114:118	arg1	flour					133:137	whole grain quinoa flour	114:137	whole grain quinoa flour	114:137	There has been growing interest in whole grain quinoa flour for new product development due to the unique nutritional benefits.
27979129	5	57	theme	enzyme	668:673	arg1	susceptibility					675:688	enzyme susceptibility	668:688	enzyme susceptibility	668:688	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	8	58	dep	starch	1155:1160	arg1	p<0.01					1172:1177	p<0.01	1172:1177	p<0.01	1172:1177	For example, peak gelatinization temperature of the flour is positively correlated to that of the starch (r=0.948, p<0.01) and negatively correlated to the lipid content (r=-0.951, p<0.01).
27979129	8	58	dep	starch	1155:1160	arg1	r=0.948					1163:1169	r=0.948	1163:1169	r=0.948	1163:1169	For example, peak gelatinization temperature of the flour is positively correlated to that of the starch (r=0.948, p<0.01) and negatively correlated to the lipid content (r=-0.951, p<0.01).
27979129	9	59	theme	other	1285:1289	arg1	components					1291:1300	other components	1285:1300	other components	1285:1300	Understanding the roles of starch and other components in physicochemical properties of quinoa flour provides a basis for better utilization of this specialty crop.
27979129	1	60	theme	grain	120:124	arg1	flour					133:137	whole grain quinoa flour	114:137	whole grain quinoa flour	114:137	There has been growing interest in whole grain quinoa flour for new product development due to the unique nutritional benefits.
27979129	1	61	from	interest	102:109	arg1	flour					133:137	whole grain quinoa flour	114:137	whole grain quinoa flour	114:137	There has been growing interest in whole grain quinoa flour for new product development due to the unique nutritional benefits.
27979129	9	62	theme	quinoa	1335:1340	arg1	flour					1342:1346	quinoa flour	1335:1346	quinoa flour	1335:1346	Understanding the roles of starch and other components in physicochemical properties of quinoa flour provides a basis for better utilization of this specialty crop.
27979129	1	63	theme	quinoa	126:131	arg1	flour					133:137	whole grain quinoa flour	114:137	whole grain quinoa flour	114:137	There has been growing interest in whole grain quinoa flour for new product development due to the unique nutritional benefits.
27979129	9	64	theme	flour	1342:1346	arg1	properties					1321:1330	physicochemical properties	1305:1330	physicochemical properties of quinoa flour	1305:1346	Understanding the roles of starch and other components in physicochemical properties of quinoa flour provides a basis for better utilization of this specialty crop.
27979129	8	65	theme	flour	1109:1113	arg1	temperature					1090:1100	peak gelatinization temperature	1070:1100	peak gelatinization temperature of the flour	1070:1113	For example, peak gelatinization temperature of the flour is positively correlated to that of the starch (r=0.948, p<0.01) and negatively correlated to the lipid content (r=-0.951, p<0.01).
27979129	6	66	theme	thermal	802:808	arg1	properties					810:819	thermal properties	802:819	thermal properties	802:819	Correlation analysis showed that thermal properties and enzyme susceptibility of quinoa flour are highly influenced by the starch.
27979129	5	67	from	variations	595:604	arg1	pasting					691:697	pasting	691:697	pasting	691:697	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	67	from	variations	595:604	arg1	composition					618:628	chemical composition	609:628	chemical composition	609:628	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	67	from	variations	595:604	arg1	properties					725:734	thermal properties	717:734	thermal properties of the flour	717:747	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	67	from	variations	595:604	arg1	index					661:665	water soluble index	647:665	water soluble index	647:665	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	67	from	variations	595:604	arg1	texture					704:710	gel texture	700:710	gel texture	700:710	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	67	from	variations	595:604	arg1	susceptibility					675:688	enzyme susceptibility	668:688	enzyme susceptibility	668:688	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	5	67	from	variations	595:604	arg1	power					640:644	swelling power	631:644	swelling power	631:644	Great variations in chemical composition, swelling power, water soluble index, enzyme susceptibility, pasting, gel texture, and thermal properties of the flour have been observed.
27979129	0	68	theme	starch	58:63	arg1	interactions					65:76	starch interactions	58:76	starch interactions	58:76	Physicochemical properties of quinoa flour as affected by starch interactions.
27979129	2	69	theme	flour	229:233	arg1	quality					211:217	The quality	207:217	The quality of quinoa flour	207:233	The quality of quinoa flour is much determined by the properties of its major component starch as well as non-starch components.
24857465	4	0	theme	surface	722:728	arg1	roughness					730:738	The surface roughness	718:738	The surface roughness of coatings with different PVA contents	718:778	The surface roughness of coatings with different PVA contents was slightly increased, while a significant increase of water contact angles due to PVA addition was detected and discussed.
24857465	2	1	theme	co-deposition	372:384	arg1	mechanism					386:394	the co-deposition mechanism	368:394	the co-deposition mechanism of the three components	368:418	The present paper discusses the co-deposition mechanism of the three components and presents a summary of the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations.
24857465	5	2	theme	PVA	976:978	arg1	PVA					976:978	PVA	976:978	PVA	976:978	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	5	2	theme	PVA	976:978	arg1	amounts					965:971	different amounts	955:971	different amounts of PVA	955:978	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	0	3	theme	composite	87:95	arg1	coating					97:103	alginate-Bioglass® composite coating	68:103	alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment	68:181	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	8	4	theme	bioactive	1460:1468	arg1	coatings					1470:1477	composite alginate-Bioglass® bioactive coatings	1431:1477	composite alginate-Bioglass® bioactive coatings combined with PVA	1431:1495	According to the results of this study, composite alginate-Bioglass® bioactive coatings combined with PVA are proposed as promising candidates for dental and orthopedic applications.
24857465	8	4	theme	bioactive	1460:1468	arg1	candidates					1523:1532	promising candidates	1513:1532	promising candidates for dental and orthopedic applications	1513:1571	According to the results of this study, composite alginate-Bioglass® bioactive coatings combined with PVA are proposed as promising candidates for dental and orthopedic applications.
24857465	2	5	with	suspensions	511:521	arg1	concentrations					542:555	different PVA concentrations	528:555	different PVA concentrations	528:555	The present paper discusses the co-deposition mechanism of the three components and presents a summary of the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations.
24857465	6	6	theme	body	1143:1146	arg1	SBF					1155:1157	SBF	1155:1157	SBF	1155:1157	In-vitro bioactivity tests were performed in simulated body fluid (SBF) for 0.5, 1, 2, 4, 7, and 14 days, respectively.
24857465	6	6	theme	body	1143:1146	arg1	fluid					1148:1152	simulated body fluid	1133:1152	simulated body fluid (SBF)	1133:1158	In-vitro bioactivity tests were performed in simulated body fluid (SBF) for 0.5, 1, 2, 4, 7, and 14 days, respectively.
24857465	0	7	theme	stainless	108:116	arg1	properties					136:145	stainless steel: mechanical properties	108:145	stainless steel: mechanical properties	108:145	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	4	8	with	roughness	730:738	arg1	contents					771:778	different PVA contents	757:778	different PVA contents	757:778	The surface roughness of coatings with different PVA contents was slightly increased, while a significant increase of water contact angles due to PVA addition was detected and discussed.
24857465	5	9	theme	adhesion	914:921	arg1	strength					923:930	Improved adhesion strength	905:930	Improved adhesion strength of coatings containing different amounts of PVA	905:978	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	4	10	theme	PVA	767:769	arg1	contents					771:778	different PVA contents	757:778	different PVA contents	757:778	The surface roughness of coatings with different PVA contents was slightly increased, while a significant increase of water contact angles due to PVA addition was detected and discussed.
24857465	3	11	theme	compact	594:600	arg1	microstructure					602:615	compact microstructure	594:615	compact microstructure	594:615	Homogeneous composite coatings with compact microstructure and increased thickness, i.e. as high as 10 μm, were observed by scanning electron microscopy (SEM).
24857465	1	12	theme	single	286:291	arg1	step					293:296	a single step	284:296	a single step electrophoretic deposition (EPD) process	284:337	PVA reinforced alginate-bioactive glass (BG) composite coatings were produced on stainless steel by a single step electrophoretic deposition (EPD) process.
24857465	4	13	theme	PVA	864:866	arg1	addition					868:875	PVA addition	864:875	PVA addition	864:875	The surface roughness of coatings with different PVA contents was slightly increased, while a significant increase of water contact angles due to PVA addition was detected and discussed.
24857465	7	14	theme	rapid	1281:1285	arg1	ability					1310:1316	rapid hydroxyapatite forming ability	1281:1316	rapid hydroxyapatite forming ability of the composite coatings in SBF	1281:1349	The decomposition rate of the coatings was reduced with PVA content, and rapid hydroxyapatite forming ability of the composite coatings in SBF was confirmed by FTIR and XRD analyses.
24857465	3	15	theme	composite	570:578	arg1	coatings					580:587	Homogeneous composite coatings	558:587	Homogeneous composite coatings with compact microstructure	558:615	Homogeneous composite coatings with compact microstructure and increased thickness, i.e. as high as 10 μm, were observed by scanning electron microscopy (SEM).
24857465	6	16	theme	simulated	1133:1141	arg1	SBF					1155:1157	SBF	1155:1157	SBF	1155:1157	In-vitro bioactivity tests were performed in simulated body fluid (SBF) for 0.5, 1, 2, 4, 7, and 14 days, respectively.
24857465	6	16	theme	simulated	1133:1141	arg1	fluid					1148:1152	simulated body fluid	1133:1152	simulated body fluid (SBF)	1133:1158	In-vitro bioactivity tests were performed in simulated body fluid (SBF) for 0.5, 1, 2, 4, 7, and 14 days, respectively.
24857465	0	17	theme	steel	118:122	arg1	properties					136:145	stainless steel: mechanical properties	108:145	stainless steel: mechanical properties	108:145	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	3	18	with	coatings	580:587	arg1	microstructure					602:615	compact microstructure	594:615	compact microstructure	594:615	Homogeneous composite coatings with compact microstructure and increased thickness, i.e. as high as 10 μm, were observed by scanning electron microscopy (SEM).
24857465	0	19	theme	Electrophoretic	0:14	arg1	co-deposition					16:28	Electrophoretic co-deposition	0:28	Electrophoretic co-deposition of polyvinyl alcohol (PVA)	0:55	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	2	20	theme	components	409:418	arg1	mechanism					386:394	the co-deposition mechanism	368:394	the co-deposition mechanism of the three components	368:418	The present paper discusses the co-deposition mechanism of the three components and presents a summary of the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations.
24857465	2	21	theme	different	528:536	arg1	concentrations					542:555	different PVA concentrations	528:555	different PVA concentrations	528:555	The present paper discusses the co-deposition mechanism of the three components and presents a summary of the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations.
24857465	3	22	theme	increased	621:629	arg1	thickness					631:639	increased thickness	621:639	increased thickness	621:639	Homogeneous composite coatings with compact microstructure and increased thickness, i.e. as high as 10 μm, were observed by scanning electron microscopy (SEM).
24857465	7	23	theme	hydroxyapatite	1287:1300	arg1	ability					1310:1316	rapid hydroxyapatite forming ability	1281:1316	rapid hydroxyapatite forming ability of the composite coatings in SBF	1281:1349	The decomposition rate of the coatings was reduced with PVA content, and rapid hydroxyapatite forming ability of the composite coatings in SBF was confirmed by FTIR and XRD analyses.
24857465	2	24	theme	properties	459:468	arg1	summary					435:441	a summary	433:441	a summary of the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations	433:555	The present paper discusses the co-deposition mechanism of the three components and presents a summary of the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations.
24857465	1	25	theme	step	293:296	arg1	process					331:337	a single step electrophoretic deposition (EPD) process	284:337	a single step electrophoretic deposition (EPD) process	284:337	PVA reinforced alginate-bioactive glass (BG) composite coatings were produced on stainless steel by a single step electrophoretic deposition (EPD) process.
24857465	4	26	theme	different	757:765	arg1	contents					771:778	different PVA contents	757:778	different PVA contents	757:778	The surface roughness of coatings with different PVA contents was slightly increased, while a significant increase of water contact angles due to PVA addition was detected and discussed.
24857465	3	27	dep	high	650:653	arg1	i.e.					642:645	i.e.	642:645	i.e.	642:645	Homogeneous composite coatings with compact microstructure and increased thickness, i.e. as high as 10 μm, were observed by scanning electron microscopy (SEM).
24857465	0	28	theme	polyvinyl	33:41	arg1	PVA					52:54	PVA	52:54	PVA	52:54	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	0	28	theme	polyvinyl	33:41	arg1	alcohol					43:49	polyvinyl alcohol	33:49	polyvinyl alcohol (PVA)	33:55	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	1	29	theme	electrophoretic	298:312	arg1	process					331:337	a single step electrophoretic deposition (EPD) process	284:337	a single step electrophoretic deposition (EPD) process	284:337	PVA reinforced alginate-bioactive glass (BG) composite coatings were produced on stainless steel by a single step electrophoretic deposition (EPD) process.
24857465	7	30	theme	composite	1325:1333	arg1	coatings					1335:1342	the composite coatings	1321:1342	the composite coatings	1321:1342	The decomposition rate of the coatings was reduced with PVA content, and rapid hydroxyapatite forming ability of the composite coatings in SBF was confirmed by FTIR and XRD analyses.
24857465	6	31	theme	In-vitro	1088:1095	arg1	tests					1109:1113	In-vitro bioactivity tests	1088:1113	In-vitro bioactivity tests	1088:1113	In-vitro bioactivity tests were performed in simulated body fluid (SBF) for 0.5, 1, 2, 4, 7, and 14 days, respectively.
24857465	5	32	theme	bending	1059:1065	arg1	tests					1067:1071	cycled bending tests	1052:1071	cycled bending tests	1052:1071	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	3	33	with	thickness	631:639	arg1	microstructure					602:615	compact microstructure	594:615	compact microstructure	594:615	Homogeneous composite coatings with compact microstructure and increased thickness, i.e. as high as 10 μm, were observed by scanning electron microscopy (SEM).
24857465	1	34	theme	deposition	314:323	arg1	process					331:337	a single step electrophoretic deposition (EPD) process	284:337	a single step electrophoretic deposition (EPD) process	284:337	PVA reinforced alginate-bioactive glass (BG) composite coatings were produced on stainless steel by a single step electrophoretic deposition (EPD) process.
24857465	0	35	theme	mechanical	125:134	arg1	properties					136:145	stainless steel: mechanical properties	108:145	stainless steel: mechanical properties	108:145	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	0	36	from	coating	97:103	arg1	assessment					172:181	in-vitro bioactivity assessment	151:181	in-vitro bioactivity assessment	151:181	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	0	36	from	coating	97:103	arg1	properties					136:145	stainless steel: mechanical properties	108:145	stainless steel: mechanical properties	108:145	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	6	37	theme	bioactivity	1097:1107	arg1	tests					1109:1113	In-vitro bioactivity tests	1088:1113	In-vitro bioactivity tests	1088:1113	In-vitro bioactivity tests were performed in simulated body fluid (SBF) for 0.5, 1, 2, 4, 7, and 14 days, respectively.
24857465	8	38	theme	dental	1538:1543	arg1	applications					1560:1571	dental and orthopedic applications	1538:1571	dental and orthopedic applications	1538:1571	According to the results of this study, composite alginate-Bioglass® bioactive coatings combined with PVA are proposed as promising candidates for dental and orthopedic applications.
24857465	0	39	theme	in-vitro	151:158	arg1	assessment					172:181	in-vitro bioactivity assessment	151:181	in-vitro bioactivity assessment	151:181	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	3	40	theme	scanning	682:689	arg1	microscopy					700:709	scanning electron microscopy	682:709	scanning electron microscopy (SEM)	682:715	Homogeneous composite coatings with compact microstructure and increased thickness, i.e. as high as 10 μm, were observed by scanning electron microscopy (SEM).
24857465	3	40	theme	scanning	682:689	arg1	SEM					712:714	SEM	712:714	SEM	712:714	Homogeneous composite coatings with compact microstructure and increased thickness, i.e. as high as 10 μm, were observed by scanning electron microscopy (SEM).
24857465	4	41	theme	contact	842:848	arg1	angles					850:855	water contact angles	836:855	water contact angles due to PVA addition	836:875	The surface roughness of coatings with different PVA contents was slightly increased, while a significant increase of water contact angles due to PVA addition was detected and discussed.
24857465	7	42	theme	forming	1302:1308	arg1	ability					1310:1316	rapid hydroxyapatite forming ability	1281:1316	rapid hydroxyapatite forming ability of the composite coatings in SBF	1281:1349	The decomposition rate of the coatings was reduced with PVA content, and rapid hydroxyapatite forming ability of the composite coatings in SBF was confirmed by FTIR and XRD analyses.
24857465	1	43	theme	composite	229:237	arg1	coatings					239:246	PVA reinforced alginate-bioactive glass (BG) composite coatings	184:246	PVA reinforced alginate-bioactive glass (BG) composite coatings	184:246	PVA reinforced alginate-bioactive glass (BG) composite coatings were produced on stainless steel by a single step electrophoretic deposition (EPD) process.
24857465	0	44	theme	alcohol	43:49	arg1	co-deposition					16:28	Electrophoretic co-deposition	0:28	Electrophoretic co-deposition of polyvinyl alcohol (PVA)	0:55	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	1	45	theme	EPD	326:328	arg1	process					331:337	a single step electrophoretic deposition (EPD) process	284:337	a single step electrophoretic deposition (EPD) process	284:337	PVA reinforced alginate-bioactive glass (BG) composite coatings were produced on stainless steel by a single step electrophoretic deposition (EPD) process.
24857465	2	46	theme	coatings	487:494	arg1	properties					459:468	the relevant properties	446:468	the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations	446:555	The present paper discusses the co-deposition mechanism of the three components and presents a summary of the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations.
24857465	8	47	theme	study	1424:1428	arg1	results					1408:1414	the results	1404:1414	the results of this study	1404:1428	According to the results of this study, composite alginate-Bioglass® bioactive coatings combined with PVA are proposed as promising candidates for dental and orthopedic applications.
24857465	2	48	theme	relevant	450:457	arg1	properties					459:468	the relevant properties	446:468	the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations	446:555	The present paper discusses the co-deposition mechanism of the three components and presents a summary of the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations.
24857465	2	49	theme	PVA	538:540	arg1	concentrations					542:555	different PVA concentrations	528:555	different PVA concentrations	528:555	The present paper discusses the co-deposition mechanism of the three components and presents a summary of the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations.
24857465	2	50	theme	composite	477:485	arg1	coatings					487:494	the composite coatings	473:494	the composite coatings deposited from suspensions with different PVA concentrations	473:555	The present paper discusses the co-deposition mechanism of the three components and presents a summary of the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations.
24857465	8	51	theme	orthopedic	1549:1558	arg1	applications					1560:1571	dental and orthopedic applications	1538:1571	dental and orthopedic applications	1538:1571	According to the results of this study, composite alginate-Bioglass® bioactive coatings combined with PVA are proposed as promising candidates for dental and orthopedic applications.
24857465	4	52	theme	due	857:859	arg1	angles					850:855	water contact angles	836:855	water contact angles due to PVA addition	836:875	The surface roughness of coatings with different PVA contents was slightly increased, while a significant increase of water contact angles due to PVA addition was detected and discussed.
24857465	8	53	theme	promising	1513:1521	arg1	candidates					1523:1532	promising candidates	1513:1532	promising candidates for dental and orthopedic applications	1513:1571	According to the results of this study, composite alginate-Bioglass® bioactive coatings combined with PVA are proposed as promising candidates for dental and orthopedic applications.
24857465	8	53	theme	promising	1513:1521	arg1	coatings					1470:1477	composite alginate-Bioglass® bioactive coatings	1431:1477	composite alginate-Bioglass® bioactive coatings combined with PVA	1431:1495	According to the results of this study, composite alginate-Bioglass® bioactive coatings combined with PVA are proposed as promising candidates for dental and orthopedic applications.
24857465	5	54	theme	coatings	935:942	arg1	strength					923:930	Improved adhesion strength	905:930	Improved adhesion strength of coatings containing different amounts of PVA	905:978	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	7	55	theme	XRD	1377:1379	arg1	analyses					1381:1388	FTIR and XRD analyses	1368:1388	FTIR and XRD analyses	1368:1388	The decomposition rate of the coatings was reduced with PVA content, and rapid hydroxyapatite forming ability of the composite coatings in SBF was confirmed by FTIR and XRD analyses.
24857465	0	56	theme	bioactivity	160:170	arg1	assessment					172:181	in-vitro bioactivity assessment	151:181	in-vitro bioactivity assessment	151:181	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	8	57	theme	composite	1431:1439	arg1	coatings					1470:1477	composite alginate-Bioglass® bioactive coatings	1431:1477	composite alginate-Bioglass® bioactive coatings combined with PVA	1431:1495	According to the results of this study, composite alginate-Bioglass® bioactive coatings combined with PVA are proposed as promising candidates for dental and orthopedic applications.
24857465	8	57	theme	composite	1431:1439	arg1	candidates					1523:1532	promising candidates	1513:1532	promising candidates for dental and orthopedic applications	1513:1571	According to the results of this study, composite alginate-Bioglass® bioactive coatings combined with PVA are proposed as promising candidates for dental and orthopedic applications.
24857465	4	58	theme	angles	850:855	arg1	increase					824:831	a significant increase	810:831	a significant increase of water contact angles due to PVA addition	810:875	The surface roughness of coatings with different PVA contents was slightly increased, while a significant increase of water contact angles due to PVA addition was detected and discussed.
24857465	5	59	contain	containing	944:953	arg2	amounts					965:971	different amounts	955:971	different amounts of PVA	955:978	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	5	59	contain	containing	944:953	arg2	PVA					976:978	PVA	976:978	PVA	976:978	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	5	59	contain	containing	944:953	arg1	coatings					935:942	coatings	935:942	coatings containing different amounts of PVA	935:978	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	7	60	theme	PVA	1264:1266	arg1	content					1268:1274	PVA content	1264:1274	PVA content	1264:1274	The decomposition rate of the coatings was reduced with PVA content, and rapid hydroxyapatite forming ability of the composite coatings in SBF was confirmed by FTIR and XRD analyses.
24857465	7	61	theme	coatings	1238:1245	arg1	rate					1226:1229	The decomposition rate	1208:1229	The decomposition rate of the coatings	1208:1245	The decomposition rate of the coatings was reduced with PVA content, and rapid hydroxyapatite forming ability of the composite coatings in SBF was confirmed by FTIR and XRD analyses.
24857465	5	62	theme	pull-off	1030:1037	arg1	adhesion					1039:1046	pull-off adhesion	1030:1046	pull-off adhesion	1030:1046	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	4	63	theme	significant	812:822	arg1	increase					824:831	a significant increase	810:831	a significant increase of water contact angles due to PVA addition	810:875	The surface roughness of coatings with different PVA contents was slightly increased, while a significant increase of water contact angles due to PVA addition was detected and discussed.
24857465	4	64	theme	water	836:840	arg1	angles					850:855	water contact angles	836:855	water contact angles due to PVA addition	836:875	The surface roughness of coatings with different PVA contents was slightly increased, while a significant increase of water contact angles due to PVA addition was detected and discussed.
24857465	2	65	theme	present	344:350	arg1	paper					352:356	The present paper	340:356	The present paper	340:356	The present paper discusses the co-deposition mechanism of the three components and presents a summary of the relevant properties of the composite coatings deposited from suspensions with different PVA concentrations.
24857465	7	66	theme	FTIR	1368:1371	arg1	analyses					1381:1388	FTIR and XRD analyses	1368:1388	FTIR and XRD analyses	1368:1388	The decomposition rate of the coatings was reduced with PVA content, and rapid hydroxyapatite forming ability of the composite coatings in SBF was confirmed by FTIR and XRD analyses.
24857465	3	67	theme	electron	691:698	arg1	microscopy					700:709	scanning electron microscopy	682:709	scanning electron microscopy (SEM)	682:715	Homogeneous composite coatings with compact microstructure and increased thickness, i.e. as high as 10 μm, were observed by scanning electron microscopy (SEM).
24857465	3	67	theme	electron	691:698	arg1	SEM					712:714	SEM	712:714	SEM	712:714	Homogeneous composite coatings with compact microstructure and increased thickness, i.e. as high as 10 μm, were observed by scanning electron microscopy (SEM).
24857465	7	68	theme	coatings	1335:1342	arg1	ability					1310:1316	rapid hydroxyapatite forming ability	1281:1316	rapid hydroxyapatite forming ability of the composite coatings in SBF	1281:1349	The decomposition rate of the coatings was reduced with PVA content, and rapid hydroxyapatite forming ability of the composite coatings in SBF was confirmed by FTIR and XRD analyses.
24857465	4	69	theme	coatings	743:750	arg1	roughness					730:738	The surface roughness	718:738	The surface roughness of coatings with different PVA contents	718:778	The surface roughness of coatings with different PVA contents was slightly increased, while a significant increase of water contact angles due to PVA addition was detected and discussed.
24857465	5	70	theme	different	955:963	arg1	PVA					976:978	PVA	976:978	PVA	976:978	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	5	70	theme	different	955:963	arg1	amounts					965:971	different amounts	955:971	different amounts of PVA	955:978	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	0	71	theme	alginate-Bioglass®	68:85	arg1	coating					97:103	alginate-Bioglass® composite coating	68:103	alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment	68:181	Electrophoretic co-deposition of polyvinyl alcohol (PVA) reinforced alginate-Bioglass® composite coating on stainless steel: mechanical properties and in-vitro bioactivity assessment.
24857465	8	72	theme	alginate-Bioglass®	1441:1458	arg1	coatings					1470:1477	composite alginate-Bioglass® bioactive coatings	1431:1477	composite alginate-Bioglass® bioactive coatings combined with PVA	1431:1495	According to the results of this study, composite alginate-Bioglass® bioactive coatings combined with PVA are proposed as promising candidates for dental and orthopedic applications.
24857465	8	72	theme	alginate-Bioglass®	1441:1458	arg1	candidates					1523:1532	promising candidates	1513:1532	promising candidates for dental and orthopedic applications	1513:1571	According to the results of this study, composite alginate-Bioglass® bioactive coatings combined with PVA are proposed as promising candidates for dental and orthopedic applications.
24857465	5	73	theme	Improved	905:912	arg1	strength					923:930	Improved adhesion strength	905:930	Improved adhesion strength of coatings containing different amounts of PVA	905:978	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	7	74	theme	decomposition	1212:1224	arg1	rate					1226:1229	The decomposition rate	1208:1229	The decomposition rate of the coatings	1208:1245	The decomposition rate of the coatings was reduced with PVA content, and rapid hydroxyapatite forming ability of the composite coatings in SBF was confirmed by FTIR and XRD analyses.
24857465	5	75	theme	cycled	1052:1057	arg1	tests					1067:1071	cycled bending tests	1052:1071	cycled bending tests	1052:1071	Improved adhesion strength of coatings containing different amounts of PVA was quantitatively and qualitatively confirmed by pull-off adhesion and cycled bending tests, respectively.
24857465	7	76	from	ability	1310:1316	arg1	SBF					1347:1349	SBF	1347:1349	SBF	1347:1349	The decomposition rate of the coatings was reduced with PVA content, and rapid hydroxyapatite forming ability of the composite coatings in SBF was confirmed by FTIR and XRD analyses.
24857465	3	77	theme	Homogeneous	558:568	arg1	coatings					580:587	Homogeneous composite coatings	558:587	Homogeneous composite coatings with compact microstructure	558:615	Homogeneous composite coatings with compact microstructure and increased thickness, i.e. as high as 10 μm, were observed by scanning electron microscopy (SEM).
24857465	1	78	theme	stainless	265:273	arg1	steel					275:279	stainless steel	265:279	stainless steel	265:279	PVA reinforced alginate-bioactive glass (BG) composite coatings were produced on stainless steel by a single step electrophoretic deposition (EPD) process.
29169988	11	0	theme	pressure	1696:1703	arg1	ulcers					1705:1710	human pressure ulcers	1690:1710	human pressure ulcers	1690:1710	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	7	1	attach	isolated	888:895	arg2	complex					876:882	A unique VG1F-SHAP-HA complex	854:882	A unique VG1F-SHAP-HA complex	854:882	A unique VG1F-SHAP-HA complex was isolated from granulation tissues using gel filtration chromatography and subsequent cesium chloride-gradient ultracentrifugation under dissociating conditions.
29169988	7	1	attach	isolated	888:895	arg1	tissues					914:920	granulation tissues	902:920	granulation tissues	902:920	A unique VG1F-SHAP-HA complex was isolated from granulation tissues using gel filtration chromatography and subsequent cesium chloride-gradient ultracentrifugation under dissociating conditions.
29169988	10	2	theme	intact	1465:1470	arg1	versican					1472:1479	intact versican	1465:1479	intact versican	1465:1479	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	4	3	theme	macrocomplex	578:589	arg1	formation					591:599	the macrocomplex formation	574:599	the macrocomplex formation of VG1F	574:607	However, the macrocomplex formation of VG1F has not been clarified.
29169988	6	4	theme	granulation	809:819	arg1	tissue					821:826	the granulation tissue	805:826	the granulation tissue	805:826	VG1F colocalized with SHAP-HA in specific regions of the granulation tissue but not with fibrillin-1.
29169988	7	5	theme	VG1F-SHAP-HA	863:874	arg1	complex					876:882	A unique VG1F-SHAP-HA complex	854:882	A unique VG1F-SHAP-HA complex	854:882	A unique VG1F-SHAP-HA complex was isolated from granulation tissues using gel filtration chromatography and subsequent cesium chloride-gradient ultracentrifugation under dissociating conditions.
29169988	11	6	theme	inflamed	1719:1726	arg1	stages					1728:1733	inflamed stages	1719:1733	inflamed stages in a mouse model of moist would healing	1719:1773	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	9	7	theme	VG1F-SHAP-HA	1270:1281	arg1	deposition					1291:1300	VG1F-SHAP-HA complex deposition	1270:1300	VG1F-SHAP-HA complex deposition	1270:1300	The addition of recombinant VG1 in fibroblast cultures enhanced VG1F-SHAP-HA complex deposition in the pericellular extracellular matrix.
29169988	3	8	theme	remodeling	524:533	arg1	tissues					535:541	remodeling tissues	524:541	remodeling tissues	524:541	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	1	9	theme	-rich	163:167	arg1	matrix					183:188	The hyaluronan (HA)-rich extracellular matrix	144:188	The hyaluronan (HA)-rich extracellular matrix	144:188	The hyaluronan (HA)-rich extracellular matrix plays dynamic roles during tissue remodeling.
29169988	9	10	from	addition	1210:1217	arg1	cultures					1252:1259	fibroblast cultures	1241:1259	fibroblast cultures	1241:1259	The addition of recombinant VG1 in fibroblast cultures enhanced VG1F-SHAP-HA complex deposition in the pericellular extracellular matrix.
29169988	6	11	theme	specific	785:792	arg1	regions					794:800	specific regions	785:800	specific regions of the granulation tissue	785:826	VG1F colocalized with SHAP-HA in specific regions of the granulation tissue but not with fibrillin-1.
29169988	3	12	theme	mature	550:555	arg1	dermis					557:562	the mature dermis	546:562	the mature dermis	546:562	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	1	13	theme	dynamic	196:202	arg1	roles					204:208	dynamic roles	196:208	dynamic roles	196:208	The hyaluronan (HA)-rich extracellular matrix plays dynamic roles during tissue remodeling.
29169988	0	14	theme	Ulcers	136:141	arg1	Tissue					117:122	Granulation Tissue	105:122	Granulation Tissue of Pressure Ulcers	105:141	The Versican G1 Fragment and Serum-Derived Hyaluronan-Associated Proteins Interact and Form a Complex in Granulation Tissue of Pressure Ulcers.
29169988	9	15	theme	extracellular	1322:1334	arg1	matrix					1336:1341	the pericellular extracellular matrix	1305:1341	the pericellular extracellular matrix	1305:1341	The addition of recombinant VG1 in fibroblast cultures enhanced VG1F-SHAP-HA complex deposition in the pericellular extracellular matrix.
29169988	5	16	theme	VG1F-containing	660:674	arg1	macrocomplex					676:687	the VG1F-containing macrocomplex	656:687	the VG1F-containing macrocomplex in pressure ulcers characterized by chronic refractory wounds	656:749	Therefore, we examined the VG1F-containing macrocomplex in pressure ulcers characterized by chronic refractory wounds.
29169988	11	17	theme	ulcers	1705:1710	arg1	tissues					1679:1685	the edematous granulation tissues	1653:1685	the edematous granulation tissues of human pressure ulcers	1653:1710	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	2	18	theme	HA-associated	263:275	arg1	molecules					378:386	major HA-binding molecules	361:386	major HA-binding molecules in remodeling processes, such as wound healing	361:433	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	18	theme	HA-associated	263:275	arg1	Versican					236:243	Versican	236:243	Versican	236:243	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	18	theme	HA-associated	263:275	arg1	SHAP					286:289	SHAP	286:289	SHAP	286:289	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	18	theme	HA-associated	263:275	arg1	protein					277:283	serum-derived HA-associated protein	249:283	serum-derived HA-associated protein (SHAP)	249:290	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	11	19	from	stages	1728:1733	arg1	model					1746:1750	a mouse model	1738:1750	a mouse model of moist	1738:1759	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	11	20	dep	stages	1728:1733	arg1	would					1761:1765	would	1761:1765	would	1761:1765	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	2	21	link	serum-derived	249:261	arg1	molecules					378:386	major HA-binding molecules	361:386	major HA-binding molecules in remodeling processes, such as wound healing	361:433	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	21	link	serum-derived	249:261	arg1	Versican					236:243	Versican	236:243	Versican	236:243	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	21	link	serum-derived	249:261	arg1	SHAP					286:289	SHAP	286:289	SHAP	286:289	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	21	link	serum-derived	249:261	arg1	protein					277:283	serum-derived HA-associated protein	249:283	serum-derived HA-associated protein (SHAP)	249:290	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	8	22	theme	recombinant	1093:1103	arg1	versican					1105:1112	recombinant versican G1, but not versican G3,	1093:1137	recombinant versican G1, but not versican G3,	1093:1137	Consistent with this molecular composition, recombinant versican G1, but not versican G3, interacted with the two heavy chains of inter-α-trypsin inhibitor.
29169988	3	23	from	present	506:512	arg1	tissues					535:541	remodeling tissues	524:541	remodeling tissues	524:541	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	3	23	from	present	506:512	arg1	dermis					557:562	the mature dermis	546:562	the mature dermis	546:562	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	11	24	theme	edematous	1657:1665	arg1	tissues					1679:1685	the edematous granulation tissues	1653:1685	the edematous granulation tissues of human pressure ulcers	1653:1710	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	5	25	theme	refractory	733:742	arg1	wounds					744:749	chronic refractory wounds	725:749	chronic refractory wounds	725:749	Therefore, we examined the VG1F-containing macrocomplex in pressure ulcers characterized by chronic refractory wounds.
29169988	7	26	theme	chloride-gradient	980:996	arg1	ultracentrifugation					998:1016	subsequent cesium chloride-gradient ultracentrifugation	962:1016	subsequent cesium chloride-gradient ultracentrifugation	962:1016	A unique VG1F-SHAP-HA complex was isolated from granulation tissues using gel filtration chromatography and subsequent cesium chloride-gradient ultracentrifugation under dissociating conditions.
29169988	3	27	theme	Versican	436:443	arg1	VG1F					465:468	VG1F	465:468	VG1F	465:468	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	3	27	theme	Versican	436:443	arg1	fragment					455:462	Versican G1-domain fragment	436:462	Versican G1-domain fragment (VG1F)	436:469	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	0	28	theme	Granulation	105:115	arg1	Tissue					117:122	Granulation Tissue	105:122	Granulation Tissue of Pressure Ulcers	105:141	The Versican G1 Fragment and Serum-Derived Hyaluronan-Associated Proteins Interact and Form a Complex in Granulation Tissue of Pressure Ulcers.
29169988	7	29	theme	subsequent	962:971	arg1	ultracentrifugation					998:1016	subsequent cesium chloride-gradient ultracentrifugation	962:1016	subsequent cesium chloride-gradient ultracentrifugation	962:1016	A unique VG1F-SHAP-HA complex was isolated from granulation tissues using gel filtration chromatography and subsequent cesium chloride-gradient ultracentrifugation under dissociating conditions.
29169988	10	30	theme	VG1F-containing	1366:1380	arg1	versican					1472:1479	intact versican	1465:1479	intact versican	1465:1479	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	30	theme	VG1F-containing	1366:1380	arg1	aggregates					1420:1429	dermal VG1F aggregates	1408:1429	dermal VG1F aggregates	1408:1429	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	30	theme	VG1F-containing	1366:1380	arg1	macrocomplexes					1382:1395	other VG1F-containing macrocomplexes	1360:1395	other VG1F-containing macrocomplexes	1360:1395	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	30	theme	VG1F-containing	1366:1380	arg1	microfibrils					1447:1458	versican-bound microfibrils	1432:1458	versican-bound microfibrils	1432:1458	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	8	31	theme	versican	1126:1133	arg1	G3					1135:1136	versican G3	1126:1136	versican G3	1126:1136	Consistent with this molecular composition, recombinant versican G1, but not versican G3, interacted with the two heavy chains of inter-α-trypsin inhibitor.
29169988	6	32	theme	tissue	821:826	arg1	regions					794:800	specific regions	785:800	specific regions of the granulation tissue	785:826	VG1F colocalized with SHAP-HA in specific regions of the granulation tissue but not with fibrillin-1.
29169988	2	33	theme	wound	421:425	arg1	healing					427:433	wound healing	421:433	wound healing	421:433	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	0	34	theme	G1	13:14	arg1	Fragment					16:23	The Versican G1 Fragment	0:23	The Versican G1 Fragment	0:23	The Versican G1 Fragment and Serum-Derived Hyaluronan-Associated Proteins Interact and Form a Complex in Granulation Tissue of Pressure Ulcers.
29169988	11	35	theme	inflammatory	1844:1855	arg1	reactions					1857:1865	inflammatory reactions	1844:1865	inflammatory reactions	1844:1865	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	10	36	theme	dermal	1408:1413	arg1	aggregates					1420:1429	dermal VG1F aggregates	1408:1429	dermal VG1F aggregates	1408:1429	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	11	37	theme	suitable	1831:1838	arg1	matrix					1824:1829	an HA-rich matrix	1813:1829	an HA-rich matrix suitable for inflammatory reactions	1813:1865	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	2	38	theme	inhibitor	346:354	arg1	chains					320:325	the heavy chains	310:325	the heavy chains of inter-α-trypsin inhibitor	310:354	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	0	39	theme	Hyaluronan-Associated	43:63	arg1	Proteins					65:72	Serum-Derived Hyaluronan-Associated Proteins	29:72	Serum-Derived Hyaluronan-Associated Proteins	29:72	The Versican G1 Fragment and Serum-Derived Hyaluronan-Associated Proteins Interact and Form a Complex in Granulation Tissue of Pressure Ulcers.
29169988	10	40	theme	formation	1559:1567	arg1	organization					1514:1525	the tissue-specific organization	1494:1525	the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP	1494:1596	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	41	theme	versican-bound	1432:1445	arg1	microfibrils					1447:1458	versican-bound microfibrils	1432:1458	versican-bound microfibrils	1432:1458	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	7	42	theme	filtration	932:941	arg1	chromatography					943:956	gel filtration chromatography	928:956	gel filtration chromatography	928:956	A unique VG1F-SHAP-HA complex was isolated from granulation tissues using gel filtration chromatography and subsequent cesium chloride-gradient ultracentrifugation under dissociating conditions.
29169988	5	43	from	macrocomplex	676:687	arg1	ulcers					701:706	pressure ulcers	692:706	pressure ulcers characterized by chronic refractory wounds	692:749	Therefore, we examined the VG1F-containing macrocomplex in pressure ulcers characterized by chronic refractory wounds.
29169988	10	44	theme	other	1360:1364	arg1	versican					1472:1479	intact versican	1465:1479	intact versican	1465:1479	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	44	theme	other	1360:1364	arg1	aggregates					1420:1429	dermal VG1F aggregates	1408:1429	dermal VG1F aggregates	1408:1429	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	44	theme	other	1360:1364	arg1	macrocomplexes					1382:1395	other VG1F-containing macrocomplexes	1360:1395	other VG1F-containing macrocomplexes	1360:1395	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	44	theme	other	1360:1364	arg1	microfibrils					1447:1458	versican-bound microfibrils	1432:1458	versican-bound microfibrils	1432:1458	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	2	45	theme	HA-binding	367:376	arg1	molecules					378:386	major HA-binding molecules	361:386	major HA-binding molecules in remodeling processes, such as wound healing	361:433	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	45	theme	HA-binding	367:376	arg1	Versican					236:243	Versican	236:243	Versican	236:243	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	45	theme	HA-binding	367:376	arg1	protein					277:283	serum-derived HA-associated protein	249:283	serum-derived HA-associated protein (SHAP)	249:290	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	8	46	theme	inter-α-trypsin	1179:1193	arg1	inhibitor					1195:1203	inter-α-trypsin inhibitor	1179:1203	inter-α-trypsin inhibitor	1179:1203	Consistent with this molecular composition, recombinant versican G1, but not versican G3, interacted with the two heavy chains of inter-α-trypsin inhibitor.
29169988	4	47	theme	VG1F	604:607	arg1	formation					591:599	the macrocomplex formation	574:599	the macrocomplex formation of VG1F	574:607	However, the macrocomplex formation of VG1F has not been clarified.
29169988	9	48	theme	VG1	1234:1236	arg1	addition					1210:1217	The addition	1206:1217	The addition of recombinant VG1 in fibroblast cultures	1206:1259	The addition of recombinant VG1 in fibroblast cultures enhanced VG1F-SHAP-HA complex deposition in the pericellular extracellular matrix.
29169988	11	49	theme	HA-rich	1816:1822	arg1	matrix					1824:1829	an HA-rich matrix	1813:1829	an HA-rich matrix suitable for inflammatory reactions	1813:1865	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	7	50	theme	granulation	902:912	arg1	tissues					914:920	granulation tissues	902:920	granulation tissues	902:920	A unique VG1F-SHAP-HA complex was isolated from granulation tissues using gel filtration chromatography and subsequent cesium chloride-gradient ultracentrifugation under dissociating conditions.
29169988	10	51	theme	matrix	1552:1557	arg1	formation					1559:1567	HA-rich extracellular matrix formation	1530:1567	HA-rich extracellular matrix formation containing versican and SHAP	1530:1596	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	9	52	theme	fibroblast	1241:1250	arg1	cultures					1252:1259	fibroblast cultures	1241:1259	fibroblast cultures	1241:1259	The addition of recombinant VG1 in fibroblast cultures enhanced VG1F-SHAP-HA complex deposition in the pericellular extracellular matrix.
29169988	2	53	from	molecules	378:386	arg1	healing					427:433	wound healing	421:433	wound healing	421:433	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	53	from	molecules	378:386	arg1	processes					402:410	remodeling processes	391:410	remodeling processes	391:410	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	11	54	theme	moist	1755:1759	arg1	model					1746:1750	a mouse model	1738:1750	a mouse model of moist	1738:1759	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	8	55	dep	versican	1105:1112	arg1	G1					1114:1115	G1	1114:1115	G1	1114:1115	Consistent with this molecular composition, recombinant versican G1, but not versican G3, interacted with the two heavy chains of inter-α-trypsin inhibitor.
29169988	8	55	dep	versican	1105:1112	arg1	G3					1135:1136	versican G3	1126:1136	versican G3	1126:1136	Consistent with this molecular composition, recombinant versican G1, but not versican G3, interacted with the two heavy chains of inter-α-trypsin inhibitor.
29169988	11	56	located	detected	1641:1648	arg2	complex					1616:1622	The VG1F-SHAP-HA complex	1599:1622	The VG1F-SHAP-HA complex	1599:1622	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	11	56	located	detected	1641:1648	arg1	tissues					1679:1685	the edematous granulation tissues	1653:1685	the edematous granulation tissues of human pressure ulcers	1653:1710	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	11	57	theme	human	1690:1694	arg1	ulcers					1705:1710	human pressure ulcers	1690:1710	human pressure ulcers	1690:1710	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	10	58	contain	containing	1569:1578	arg2	SHAP					1593:1596	SHAP	1593:1596	SHAP	1593:1596	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	58	contain	containing	1569:1578	arg2	versican					1580:1587	versican	1580:1587	versican	1580:1587	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	58	contain	containing	1569:1578	arg1	formation					1559:1567	HA-rich extracellular matrix formation	1530:1567	HA-rich extracellular matrix formation containing versican and SHAP	1530:1596	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	8	59	theme	molecular	1070:1078	arg1	composition					1080:1090	this molecular composition	1065:1090	this molecular composition	1065:1090	Consistent with this molecular composition, recombinant versican G1, but not versican G3, interacted with the two heavy chains of inter-α-trypsin inhibitor.
29169988	9	60	theme	complex	1283:1289	arg1	deposition					1291:1300	VG1F-SHAP-HA complex deposition	1270:1300	VG1F-SHAP-HA complex deposition	1270:1300	The addition of recombinant VG1 in fibroblast cultures enhanced VG1F-SHAP-HA complex deposition in the pericellular extracellular matrix.
29169988	2	61	theme	heavy	314:318	arg1	chains					320:325	the heavy chains	310:325	the heavy chains of inter-α-trypsin inhibitor	310:354	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	7	62	theme	unique	856:861	arg1	complex					876:882	A unique VG1F-SHAP-HA complex	854:882	A unique VG1F-SHAP-HA complex	854:882	A unique VG1F-SHAP-HA complex was isolated from granulation tissues using gel filtration chromatography and subsequent cesium chloride-gradient ultracentrifugation under dissociating conditions.
29169988	1	63	theme	extracellular	169:181	arg1	matrix					183:188	The hyaluronan (HA)-rich extracellular matrix	144:188	The hyaluronan (HA)-rich extracellular matrix	144:188	The hyaluronan (HA)-rich extracellular matrix plays dynamic roles during tissue remodeling.
29169988	10	64	theme	extracellular	1538:1550	arg1	formation					1559:1567	HA-rich extracellular matrix formation	1530:1567	HA-rich extracellular matrix formation containing versican and SHAP	1530:1596	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	0	65	theme	Pressure	127:134	arg1	Ulcers					136:141	Pressure Ulcers	127:141	Pressure Ulcers	127:141	The Versican G1 Fragment and Serum-Derived Hyaluronan-Associated Proteins Interact and Form a Complex in Granulation Tissue of Pressure Ulcers.
29169988	10	66	theme	HA-rich	1530:1536	arg1	formation					1559:1567	HA-rich extracellular matrix formation	1530:1567	HA-rich extracellular matrix formation containing versican and SHAP	1530:1596	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	9	67	theme	pericellular	1309:1320	arg1	matrix					1336:1341	the pericellular extracellular matrix	1305:1341	the pericellular extracellular matrix	1305:1341	The addition of recombinant VG1 in fibroblast cultures enhanced VG1F-SHAP-HA complex deposition in the pericellular extracellular matrix.
29169988	10	68	with	Comparison	1344:1353	arg1	versican					1472:1479	intact versican	1465:1479	intact versican	1465:1479	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	68	with	Comparison	1344:1353	arg1	aggregates					1420:1429	dermal VG1F aggregates	1408:1429	dermal VG1F aggregates	1408:1429	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	68	with	Comparison	1344:1353	arg1	macrocomplexes					1382:1395	other VG1F-containing macrocomplexes	1360:1395	other VG1F-containing macrocomplexes	1360:1395	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	10	68	with	Comparison	1344:1353	arg1	microfibrils					1447:1458	versican-bound microfibrils	1432:1458	versican-bound microfibrils	1432:1458	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	3	69	located	present	506:512	arg1	tissues					535:541	remodeling tissues	524:541	remodeling tissues	524:541	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	3	69	located	present	506:512	arg2	VG1F					465:468	VG1F	465:468	VG1F	465:468	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	3	69	located	present	506:512	arg1	dermis					557:562	the mature dermis	546:562	the mature dermis	546:562	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	3	69	located	present	506:512	arg2	fragment					455:462	Versican G1-domain fragment	436:462	Versican G1-domain fragment (VG1F)	436:469	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	5	70	theme	pressure	692:699	arg1	ulcers					701:706	pressure ulcers	692:706	pressure ulcers characterized by chronic refractory wounds	692:749	Therefore, we examined the VG1F-containing macrocomplex in pressure ulcers characterized by chronic refractory wounds.
29169988	3	71	from	dermis	557:562	arg1	present					506:512	present	506:512	present	506:512	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	2	72	theme	serum-derived	249:261	arg1	molecules					378:386	major HA-binding molecules	361:386	major HA-binding molecules in remodeling processes, such as wound healing	361:433	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	72	theme	serum-derived	249:261	arg1	Versican					236:243	Versican	236:243	Versican	236:243	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	72	theme	serum-derived	249:261	arg1	SHAP					286:289	SHAP	286:289	SHAP	286:289	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	72	theme	serum-derived	249:261	arg1	protein					277:283	serum-derived HA-associated protein	249:283	serum-derived HA-associated protein (SHAP)	249:290	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	8	73	with	Consistent	1049:1058	arg1	composition					1080:1090	this molecular composition	1065:1090	this molecular composition	1065:1090	Consistent with this molecular composition, recombinant versican G1, but not versican G3, interacted with the two heavy chains of inter-α-trypsin inhibitor.
29169988	1	74	theme	tissue	217:222	arg1	remodeling					224:233	tissue remodeling	217:233	tissue remodeling	217:233	The hyaluronan (HA)-rich extracellular matrix plays dynamic roles during tissue remodeling.
29169988	11	75	theme	granulation	1667:1677	arg1	tissues					1679:1685	the edematous granulation tissues	1653:1685	the edematous granulation tissues of human pressure ulcers	1653:1710	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	3	76	from	tissues	535:541	arg1	present					506:512	present	506:512	present	506:512	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	5	77	theme	chronic	725:731	arg1	wounds					744:749	chronic refractory wounds	725:749	chronic refractory wounds	725:749	Therefore, we examined the VG1F-containing macrocomplex in pressure ulcers characterized by chronic refractory wounds.
29169988	7	78	theme	dissociating	1024:1035	arg1	conditions					1037:1046	dissociating conditions	1024:1046	dissociating conditions	1024:1046	A unique VG1F-SHAP-HA complex was isolated from granulation tissues using gel filtration chromatography and subsequent cesium chloride-gradient ultracentrifugation under dissociating conditions.
29169988	3	79	theme	G1-domain	445:453	arg1	VG1F					465:468	VG1F	465:468	VG1F	465:468	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	3	79	theme	G1-domain	445:453	arg1	fragment					455:462	Versican G1-domain fragment	436:462	Versican G1-domain fragment (VG1F)	436:469	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	0	80	theme	Versican	4:11	arg1	Fragment					16:23	The Versican G1 Fragment	0:23	The Versican G1 Fragment	0:23	The Versican G1 Fragment and Serum-Derived Hyaluronan-Associated Proteins Interact and Form a Complex in Granulation Tissue of Pressure Ulcers.
29169988	7	81	theme	cesium	973:978	arg1	ultracentrifugation					998:1016	subsequent cesium chloride-gradient ultracentrifugation	962:1016	subsequent cesium chloride-gradient ultracentrifugation	962:1016	A unique VG1F-SHAP-HA complex was isolated from granulation tissues using gel filtration chromatography and subsequent cesium chloride-gradient ultracentrifugation under dissociating conditions.
29169988	11	82	theme	VG1F-SHAP-HA	1603:1614	arg1	complex					1616:1622	The VG1F-SHAP-HA complex	1599:1622	The VG1F-SHAP-HA complex	1599:1622	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	10	83	theme	VG1F	1415:1418	arg1	aggregates					1420:1429	dermal VG1F aggregates	1408:1429	dermal VG1F aggregates	1408:1429	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	0	84	theme	Serum-Derived	29:41	arg1	Proteins					65:72	Serum-Derived Hyaluronan-Associated Proteins	29:72	Serum-Derived Hyaluronan-Associated Proteins	29:72	The Versican G1 Fragment and Serum-Derived Hyaluronan-Associated Proteins Interact and Form a Complex in Granulation Tissue of Pressure Ulcers.
29169988	11	85	theme	mouse	1740:1744	arg1	model					1746:1750	a mouse model	1738:1750	a mouse model of moist	1738:1759	The VG1F-SHAP-HA complex was specifically detected in the edematous granulation tissues of human pressure ulcers and in inflamed stages in a mouse model of moist would healing, suggesting that the complex provides an HA-rich matrix suitable for inflammatory reactions.
29169988	8	86	theme	heavy	1163:1167	arg1	chains					1169:1174	the two heavy chains	1155:1174	the two heavy chains of inter-α-trypsin inhibitor	1155:1203	Consistent with this molecular composition, recombinant versican G1, but not versican G3, interacted with the two heavy chains of inter-α-trypsin inhibitor.
29169988	3	87	attach	present	506:512	arg1	tissues					535:541	remodeling tissues	524:541	remodeling tissues	524:541	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	3	87	attach	present	506:512	arg2	VG1F					465:468	VG1F	465:468	VG1F	465:468	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	3	87	attach	present	506:512	arg1	dermis					557:562	the mature dermis	546:562	the mature dermis	546:562	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	3	87	attach	present	506:512	arg2	fragment					455:462	Versican G1-domain fragment	436:462	Versican G1-domain fragment (VG1F)	436:469	Versican G1-domain fragment (VG1F) is generated by proteolysis and is present in either remodeling tissues or the mature dermis.
29169988	2	88	theme	inter-α-trypsin	330:344	arg1	inhibitor					346:354	inter-α-trypsin inhibitor	330:354	inter-α-trypsin inhibitor	330:354	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	7	89	theme	gel	928:930	arg1	chromatography					943:956	gel filtration chromatography	928:956	gel filtration chromatography	928:956	A unique VG1F-SHAP-HA complex was isolated from granulation tissues using gel filtration chromatography and subsequent cesium chloride-gradient ultracentrifugation under dissociating conditions.
29169988	8	90	theme	inhibitor	1195:1203	arg1	chains					1169:1174	the two heavy chains	1155:1174	the two heavy chains of inter-α-trypsin inhibitor	1155:1203	Consistent with this molecular composition, recombinant versican G1, but not versican G3, interacted with the two heavy chains of inter-α-trypsin inhibitor.
29169988	10	91	theme	tissue-specific	1498:1512	arg1	organization					1514:1525	the tissue-specific organization	1494:1525	the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP	1494:1596	Comparison with other VG1F-containing macrocomplexes, including dermal VG1F aggregates, versican-bound microfibrils, and intact versican, highlighted the tissue-specific organization of HA-rich extracellular matrix formation containing versican and SHAP.
29169988	2	92	theme	remodeling	391:400	arg1	healing					427:433	wound healing	421:433	wound healing	421:433	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	92	theme	remodeling	391:400	arg1	processes					402:410	remodeling processes	391:410	remodeling processes	391:410	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	9	93	theme	recombinant	1222:1232	arg1	VG1					1234:1236	recombinant VG1	1222:1236	recombinant VG1	1222:1236	The addition of recombinant VG1 in fibroblast cultures enhanced VG1F-SHAP-HA complex deposition in the pericellular extracellular matrix.
29169988	2	94	theme	major	361:365	arg1	molecules					378:386	major HA-binding molecules	361:386	major HA-binding molecules in remodeling processes, such as wound healing	361:433	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	94	theme	major	361:365	arg1	Versican					236:243	Versican	236:243	Versican	236:243	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
29169988	2	94	theme	major	361:365	arg1	protein					277:283	serum-derived HA-associated protein	249:283	serum-derived HA-associated protein (SHAP)	249:290	Versican and serum-derived HA-associated protein (SHAP), corresponding to the heavy chains of inter-α-trypsin inhibitor, are major HA-binding molecules in remodeling processes, such as wound healing.
28259094	1	0	theme	first	435:439	arg1	metabolism					446:455	the intensive first pass metabolism	421:455	the intensive first pass metabolism	421:455	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	1	1	theme	metabolism	446:455	arg1	circumvention					404:416	the circumvention	400:416	the circumvention of the intensive first pass metabolism	400:455	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	1	2	theme	Papaverine	177:186	arg1	hydrochloride					188:200	Papaverine hydrochloride	177:200	Papaverine hydrochloride loaded electrospun fibers	177:226	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	4	3	theme	polymers	774:781	arg1	ratio					757:761	only the ratio	748:761	only the ratio of the two polymers	748:781	During the experiments, the total polymer concentration was kept constantly at 15% (w/w), and only the ratio of the two polymers was changed.
28259094	11	4	theme	samples	1765:1771	arg1	characteristics					1742:1756	the best fiber characteristics	1727:1756	the best fiber characteristics of the samples	1727:1771	Polymer solutions of the lowest elasticity and smallest intermolecular interactions contributed to the best fiber characteristics of the samples.
28259094	1	5	theme	loaded	202:207	arg1	fibers					221:226	Papaverine hydrochloride loaded electrospun fibers	177:226	Papaverine hydrochloride loaded electrospun fibers	177:226	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	8	6	theme	electrospinnability	1298:1316	arg1	properties					1252:1261	the macrostructural properties	1232:1261	the macrostructural properties of the polymer solutions and their electrospinnability	1232:1316	A correlation was found between the macrostructural properties of the polymer solutions and their electrospinnability and the consequent morphology of the resultant samples.
28259094	8	6	theme	electrospinnability	1298:1316	arg1	morphology					1337:1346	the consequent morphology	1322:1346	the consequent morphology of the resultant samples	1322:1371	A correlation was found between the macrostructural properties of the polymer solutions and their electrospinnability and the consequent morphology of the resultant samples.
28259094	9	7	theme	ratio	1412:1416	arg1	changes					1389:1395	the changes	1385:1395	the changes of the polymer ratio	1385:1416	Along with the changes of the polymer ratio, the corresponding morphology of the electrospun samples also varied.
28259094	10	8	theme	film-like	1563:1571	arg1	structure					1573:1581	the spray-dried film-like structure	1547:1581	the spray-dried film-like structure	1547:1581	With decreasing HPC ratio of the system, a transition from the spray-dried film-like structure through fibrous film to fibers was observed.
28259094	13	9	theme	6:4	1928:1930	arg1	ratios					1940:1945	6:4 polymer ratios	1928:1945	6:4 polymer ratios	1928:1945	According to the results 5:5 and 6:4 polymer ratios enabled the best fiber performance.
28259094	4	10	theme	total	682:686	arg1	concentration					696:708	the total polymer concentration	678:708	the total polymer concentration	678:708	During the experiments, the total polymer concentration was kept constantly at 15% (w/w), and only the ratio of the two polymers was changed.
28259094	0	11	theme	fiber	160:164	arg1	formation					166:174	electrospun fiber formation	148:174	electrospun fiber formation	148:174	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	3	12	theme	poly	618:621	arg1	alcohol					629:635	the also mucoadhesive poly(vinyl alcohol)	596:636	the also mucoadhesive poly(vinyl alcohol) (PVA)	596:642	In order to improve the electrospinnability of HPC, the also mucoadhesive poly(vinyl alcohol) (PVA) was used.
28259094	3	12	theme	poly	618:621	arg1	PVA					639:641	PVA	639:641	PVA	639:641	In order to improve the electrospinnability of HPC, the also mucoadhesive poly(vinyl alcohol) (PVA) was used.
28259094	6	13	theme	measurements	894:905	arg1	Combination					867:877	Combination	867:877	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy	867:978	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	0	14	from	solution	79:86	arg1	Prediction					0:9	Prediction	0:9	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug	0:139	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	6	15	with	Combination	867:877	arg1	microscopy					969:978	scanning electron microscopy	951:978	scanning electron microscopy	951:978	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	11	16	theme	intermolecular	1684:1697	arg1	interactions					1699:1710	smallest intermolecular interactions	1675:1710	smallest intermolecular interactions	1675:1710	Polymer solutions of the lowest elasticity and smallest intermolecular interactions contributed to the best fiber characteristics of the samples.
28259094	9	17	theme	samples	1467:1473	arg1	morphology					1437:1446	the corresponding morphology	1419:1446	the corresponding morphology of the electrospun samples	1419:1473	Along with the changes of the polymer ratio, the corresponding morphology of the electrospun samples also varied.
28259094	11	18	theme	fiber	1736:1740	arg1	characteristics					1742:1756	the best fiber characteristics	1727:1756	the best fiber characteristics of the samples	1727:1771	Polymer solutions of the lowest elasticity and smallest intermolecular interactions contributed to the best fiber characteristics of the samples.
28259094	12	19	theme	electrospun	1875:1885	arg1	fibers					1887:1892	electrospun fibers	1875:1892	electrospun fibers	1875:1892	The results enable the determination of the polymer ratio for the formation of applicable quality of electrospun fibers.
28259094	5	20	theme	PVA	815:817	arg1	ratios					819:824	Five different HPC:PVA ratios	796:824	Five different HPC:PVA ratios (5:5, 6:4, 7:3, 8:2, 9:1)	796:850	Five different HPC:PVA ratios (5:5, 6:4, 7:3, 8:2, 9:1) were examined.
28259094	0	21	from	Prediction	0:9	arg1	solution					79:86	aqueous solution	71:86	aqueous solution containing papaverine hydrochloride in terms of drug	71:139	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	13	22	theme	polymer	1932:1938	arg1	ratios					1940:1945	6:4 polymer ratios	1928:1945	6:4 polymer ratios	1928:1945	According to the results 5:5 and 6:4 polymer ratios enabled the best fiber performance.
28259094	8	23	theme	solutions	1278:1286	arg1	properties					1252:1261	the macrostructural properties	1232:1261	the macrostructural properties of the polymer solutions and their electrospinnability	1232:1316	A correlation was found between the macrostructural properties of the polymer solutions and their electrospinnability and the consequent morphology of the resultant samples.
28259094	8	23	theme	solutions	1278:1286	arg1	morphology					1337:1346	the consequent morphology	1322:1346	the consequent morphology of the resultant samples	1322:1371	A correlation was found between the macrostructural properties of the polymer solutions and their electrospinnability and the consequent morphology of the resultant samples.
28259094	7	24	theme	papaverine	1114:1123	arg1	hydrochloride					1125:1137	papaverine hydrochloride	1114:1137	papaverine hydrochloride	1114:1137	The crystalline-amorphous transition of papaverine hydrochloride was also tracked by Fourier transform infrared spectroscopy.
28259094	5	25	dep	ratios	819:824	arg1	7:3					837:839	7:3	837:839	7:3	837:839	Five different HPC:PVA ratios (5:5, 6:4, 7:3, 8:2, 9:1) were examined.
28259094	5	25	dep	ratios	819:824	arg1	9:1					847:849	9:1	847:849	9:1	847:849	Five different HPC:PVA ratios (5:5, 6:4, 7:3, 8:2, 9:1) were examined.
28259094	5	25	dep	ratios	819:824	arg1	6:4					832:834	6:4	832:834	6:4	832:834	Five different HPC:PVA ratios (5:5, 6:4, 7:3, 8:2, 9:1) were examined.
28259094	5	25	dep	ratios	819:824	arg1	5:5					827:829	5:5	827:829	5:5	827:829	Five different HPC:PVA ratios (5:5, 6:4, 7:3, 8:2, 9:1) were examined.
28259094	5	25	dep	ratios	819:824	arg1	8:2					842:844	8:2	842:844	8:2	842:844	Five different HPC:PVA ratios (5:5, 6:4, 7:3, 8:2, 9:1) were examined.
28259094	0	26	theme	papaverine	99:108	arg1	hydrochloride					110:122	papaverine hydrochloride	99:122	papaverine hydrochloride	99:122	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	10	27	theme	fibrous	1591:1597	arg1	film					1599:1602	fibrous film	1591:1602	fibrous film	1591:1602	With decreasing HPC ratio of the system, a transition from the spray-dried film-like structure through fibrous film to fibers was observed.
28259094	12	28	theme	quality	1864:1870	arg1	formation					1840:1848	the formation	1836:1848	the formation of applicable quality of electrospun fibers	1836:1892	The results enable the determination of the polymer ratio for the formation of applicable quality of electrospun fibers.
28259094	7	29	dep	Fourier	1159:1165	arg1	transform					1167:1175	transform	1167:1175	transform infrared spectroscopy	1167:1197	The crystalline-amorphous transition of papaverine hydrochloride was also tracked by Fourier transform infrared spectroscopy.
28259094	6	30	theme	scanning	951:958	arg1	microscopy					969:978	scanning electron microscopy	951:978	scanning electron microscopy	951:978	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	8	31	theme	consequent	1326:1335	arg1	morphology					1337:1346	the consequent morphology	1322:1346	the consequent morphology of the resultant samples	1322:1371	A correlation was found between the macrostructural properties of the polymer solutions and their electrospinnability and the consequent morphology of the resultant samples.
28259094	1	32	theme	oral	297:300	arg1	bioavailability					302:316	the oral bioavailability	293:316	the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism	293:455	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	12	33	theme	ratio	1826:1830	arg1	determination					1797:1809	the determination	1793:1809	the determination of the polymer ratio for the formation of applicable quality of electrospun fibers	1793:1892	The results enable the determination of the polymer ratio for the formation of applicable quality of electrospun fibers.
28259094	6	34	theme	characterization	929:944	arg1	Combination					867:877	Combination	867:877	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy	867:978	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	2	35	theme	hydroxypropyl	476:488	arg1	HPC					501:503	HPC	501:503	HPC	501:503	The water soluble hydroxypropyl cellulose (HPC) was chosen as a mucoadhesive polymer.
28259094	2	35	theme	hydroxypropyl	476:488	arg1	cellulose					490:498	The water soluble hydroxypropyl cellulose	458:498	The water soluble hydroxypropyl cellulose (HPC)	458:504	The water soluble hydroxypropyl cellulose (HPC) was chosen as a mucoadhesive polymer.
28259094	2	35	theme	hydroxypropyl	476:488	arg1	polymer					535:541	a mucoadhesive polymer	520:541	a mucoadhesive polymer	520:541	The water soluble hydroxypropyl cellulose (HPC) was chosen as a mucoadhesive polymer.
28259094	0	36	theme	cellulose-poly	32:45	arg1	ratio					62:66	the hydroxypropyl cellulose-poly(vinyl alcohol) ratio	14:66	the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug	14:139	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	6	37	theme	molar	911:915	arg1	characterization					929:944	molar reflectance characterization	911:944	molar reflectance characterization	911:944	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	5	38	theme	different	801:809	arg1	ratios					819:824	Five different HPC:PVA ratios	796:824	Five different HPC:PVA ratios (5:5, 6:4, 7:3, 8:2, 9:1)	796:850	Five different HPC:PVA ratios (5:5, 6:4, 7:3, 8:2, 9:1) were examined.
28259094	3	39	theme	vinyl	623:627	arg1	alcohol					629:635	the also mucoadhesive poly(vinyl alcohol)	596:636	the also mucoadhesive poly(vinyl alcohol) (PVA)	596:642	In order to improve the electrospinnability of HPC, the also mucoadhesive poly(vinyl alcohol) (PVA) was used.
28259094	3	39	theme	vinyl	623:627	arg1	PVA					639:641	PVA	639:641	PVA	639:641	In order to improve the electrospinnability of HPC, the also mucoadhesive poly(vinyl alcohol) (PVA) was used.
28259094	8	40	theme	resultant	1355:1363	arg1	samples					1365:1371	the resultant samples	1351:1371	the resultant samples	1351:1371	A correlation was found between the macrostructural properties of the polymer solutions and their electrospinnability and the consequent morphology of the resultant samples.
28259094	0	41	theme	vinyl	47:51	arg1	cellulose-poly					32:45	hydroxypropyl cellulose-poly	18:45	the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug	14:139	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	0	41	theme	vinyl	47:51	arg1	alcohol					53:59	vinyl alcohol	47:59	vinyl alcohol	47:59	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	1	42	theme	crystalline	325:335	arg1	drug					337:340	the crystalline drug	321:340	the crystalline drug	321:340	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	6	43	theme	optimum	1021:1027	arg1	composition					1029:1039	the optimum composition	1017:1039	the optimum composition of the gels for fiber formation	1017:1071	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	11	44	theme	smallest	1675:1682	arg1	interactions					1699:1710	smallest intermolecular interactions	1675:1710	smallest intermolecular interactions	1675:1710	Polymer solutions of the lowest elasticity and smallest intermolecular interactions contributed to the best fiber characteristics of the samples.
28259094	0	45	contain	containing	88:97	arg2	hydrochloride					110:122	papaverine hydrochloride	99:122	papaverine hydrochloride	99:122	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	0	45	contain	containing	88:97	arg1	solution					79:86	aqueous solution	71:86	aqueous solution containing papaverine hydrochloride in terms of drug	71:139	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	5	46	theme	HPC	811:813	arg1	ratios					819:824	Five different HPC:PVA ratios	796:824	Five different HPC:PVA ratios (5:5, 6:4, 7:3, 8:2, 9:1)	796:850	Five different HPC:PVA ratios (5:5, 6:4, 7:3, 8:2, 9:1) were examined.
28259094	3	47	used	used	648:651	arg2	PVA					639:641	PVA	639:641	PVA	639:641	In order to improve the electrospinnability of HPC, the also mucoadhesive poly(vinyl alcohol) (PVA) was used.
28259094	3	47	used	used	648:651	arg2	alcohol					629:635	the also mucoadhesive poly(vinyl alcohol)	596:636	the also mucoadhesive poly(vinyl alcohol) (PVA)	596:642	In order to improve the electrospinnability of HPC, the also mucoadhesive poly(vinyl alcohol) (PVA) was used.
28259094	10	48	theme	HPC	1504:1506	arg1	ratio					1508:1512	HPC ratio	1504:1512	HPC ratio of the system	1504:1526	With decreasing HPC ratio of the system, a transition from the spray-dried film-like structure through fibrous film to fibers was observed.
28259094	11	49	theme	elasticity	1660:1669	arg1	solutions					1636:1644	Polymer solutions	1628:1644	Polymer solutions of the lowest elasticity and smallest intermolecular interactions	1628:1710	Polymer solutions of the lowest elasticity and smallest intermolecular interactions contributed to the best fiber characteristics of the samples.
28259094	1	50	theme	drug	253:256	arg1	delivery					258:265	buccal drug delivery	246:265	buccal drug delivery	246:265	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	7	51	theme	hydrochloride	1125:1137	arg1	transition					1100:1109	The crystalline-amorphous transition	1074:1109	The crystalline-amorphous transition of papaverine hydrochloride	1074:1137	The crystalline-amorphous transition of papaverine hydrochloride was also tracked by Fourier transform infrared spectroscopy.
28259094	13	52	theme	fiber	1964:1968	arg1	performance					1970:1980	the best fiber performance	1955:1980	the best fiber performance	1955:1980	According to the results 5:5 and 6:4 polymer ratios enabled the best fiber performance.
28259094	10	53	theme	system	1521:1526	arg1	ratio					1508:1512	HPC ratio	1504:1512	HPC ratio of the system	1504:1526	With decreasing HPC ratio of the system, a transition from the spray-dried film-like structure through fibrous film to fibers was observed.
28259094	1	54	theme	pass	441:444	arg1	metabolism					446:455	the intensive first pass metabolism	421:455	the intensive first pass metabolism	421:455	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	10	55	from	structure	1573:1581	arg1	transition					1531:1540	a transition	1529:1540	a transition from the spray-dried film-like structure through fibrous film to fibers	1529:1612	With decreasing HPC ratio of the system, a transition from the spray-dried film-like structure through fibrous film to fibers was observed.
28259094	6	56	theme	fiber	1057:1061	arg1	formation					1063:1071	fiber formation	1057:1071	fiber formation	1057:1071	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	1	57	theme	hydrochloride	188:200	arg1	fibers					221:226	Papaverine hydrochloride loaded electrospun fibers	177:226	Papaverine hydrochloride loaded electrospun fibers	177:226	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	6	58	theme	gels	1048:1051	arg1	composition					1029:1039	the optimum composition	1017:1039	the optimum composition of the gels for fiber formation	1017:1071	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	9	59	theme	polymer	1404:1410	arg1	ratio					1412:1416	the polymer ratio	1400:1416	the polymer ratio	1400:1416	Along with the changes of the polymer ratio, the corresponding morphology of the electrospun samples also varied.
28259094	0	60	theme	drug	136:139	arg1	terms					127:131	terms	127:131	terms of drug	127:139	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	3	61	theme	HPC	591:593	arg1	electrospinnability					568:586	the electrospinnability	564:586	the electrospinnability of HPC	564:593	In order to improve the electrospinnability of HPC, the also mucoadhesive poly(vinyl alcohol) (PVA) was used.
28259094	2	62	theme	mucoadhesive	522:533	arg1	cellulose					490:498	The water soluble hydroxypropyl cellulose	458:498	The water soluble hydroxypropyl cellulose (HPC)	458:504	The water soluble hydroxypropyl cellulose (HPC) was chosen as a mucoadhesive polymer.
28259094	2	62	theme	mucoadhesive	522:533	arg1	polymer					535:541	a mucoadhesive polymer	520:541	a mucoadhesive polymer	520:541	The water soluble hydroxypropyl cellulose (HPC) was chosen as a mucoadhesive polymer.
28259094	0	63	from	ratio	62:66	arg1	solution					79:86	aqueous solution	71:86	aqueous solution containing papaverine hydrochloride in terms of drug	71:139	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	10	64	theme	spray-dried	1551:1561	arg1	structure					1573:1581	the spray-dried film-like structure	1547:1581	the spray-dried film-like structure	1547:1581	With decreasing HPC ratio of the system, a transition from the spray-dried film-like structure through fibrous film to fibers was observed.
28259094	0	65	theme	electrospun	148:158	arg1	formation					166:174	electrospun fiber formation	148:174	electrospun fiber formation	148:174	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	9	66	theme	corresponding	1423:1435	arg1	morphology					1437:1446	the corresponding morphology	1419:1446	the corresponding morphology of the electrospun samples	1419:1473	Along with the changes of the polymer ratio, the corresponding morphology of the electrospun samples also varied.
28259094	3	67	theme	mucoadhesive	605:616	arg1	alcohol					629:635	the also mucoadhesive poly(vinyl alcohol)	596:636	the also mucoadhesive poly(vinyl alcohol) (PVA)	596:642	In order to improve the electrospinnability of HPC, the also mucoadhesive poly(vinyl alcohol) (PVA) was used.
28259094	3	67	theme	mucoadhesive	605:616	arg1	PVA					639:641	PVA	639:641	PVA	639:641	In order to improve the electrospinnability of HPC, the also mucoadhesive poly(vinyl alcohol) (PVA) was used.
28259094	0	68	theme	ratio	62:66	arg1	Prediction					0:9	Prediction	0:9	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug	0:139	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	1	69	theme	intensive	425:433	arg1	metabolism					446:455	the intensive first pass metabolism	421:455	the intensive first pass metabolism	421:455	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	1	70	theme	electrospun	209:219	arg1	fibers					221:226	Papaverine hydrochloride loaded electrospun fibers	177:226	Papaverine hydrochloride loaded electrospun fibers	177:226	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	9	71	theme	electrospun	1455:1465	arg1	samples					1467:1473	the electrospun samples	1451:1473	the electrospun samples	1451:1473	Along with the changes of the polymer ratio, the corresponding morphology of the electrospun samples also varied.
28259094	6	72	theme	rheological	882:892	arg1	measurements					894:905	rheological measurements	882:905	rheological measurements	882:905	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	8	73	theme	macrostructural	1236:1250	arg1	properties					1252:1261	the macrostructural properties	1232:1261	the macrostructural properties of the polymer solutions and their electrospinnability	1232:1316	A correlation was found between the macrostructural properties of the polymer solutions and their electrospinnability and the consequent morphology of the resultant samples.
28259094	11	74	theme	interactions	1699:1710	arg1	solutions					1636:1644	Polymer solutions	1628:1644	Polymer solutions of the lowest elasticity and smallest intermolecular interactions	1628:1710	Polymer solutions of the lowest elasticity and smallest intermolecular interactions contributed to the best fiber characteristics of the samples.
28259094	0	75	theme	aqueous	71:77	arg1	solution					79:86	aqueous solution	71:86	aqueous solution containing papaverine hydrochloride in terms of drug	71:139	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	12	76	theme	fibers	1887:1892	arg1	quality					1864:1870	applicable quality	1853:1870	applicable quality of electrospun fibers	1853:1892	The results enable the determination of the polymer ratio for the formation of applicable quality of electrospun fibers.
28259094	8	77	theme	polymer	1270:1276	arg1	solutions					1278:1286	the polymer solutions	1266:1286	the polymer solutions	1266:1286	A correlation was found between the macrostructural properties of the polymer solutions and their electrospinnability and the consequent morphology of the resultant samples.
28259094	11	78	theme	best	1731:1734	arg1	characteristics					1742:1756	the best fiber characteristics	1727:1756	the best fiber characteristics of the samples	1727:1771	Polymer solutions of the lowest elasticity and smallest intermolecular interactions contributed to the best fiber characteristics of the samples.
28259094	7	79	theme	crystalline-amorphous	1078:1098	arg1	transition					1100:1109	The crystalline-amorphous transition	1074:1109	The crystalline-amorphous transition of papaverine hydrochloride	1074:1137	The crystalline-amorphous transition of papaverine hydrochloride was also tracked by Fourier transform infrared spectroscopy.
28259094	12	80	theme	applicable	1853:1862	arg1	quality					1864:1870	applicable quality	1853:1870	applicable quality of electrospun fibers	1853:1892	The results enable the determination of the polymer ratio for the formation of applicable quality of electrospun fibers.
28259094	1	81	theme	increased	372:380	arg1	solubility					382:391	the increased solubility	368:391	the increased solubility	368:391	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	6	82	theme	electron	960:967	arg1	microscopy					969:978	scanning electron microscopy	951:978	scanning electron microscopy	951:978	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	4	83	theme	polymer	688:694	arg1	concentration					696:708	the total polymer concentration	678:708	the total polymer concentration	678:708	During the experiments, the total polymer concentration was kept constantly at 15% (w/w), and only the ratio of the two polymers was changed.
28259094	0	84	theme	hydroxypropyl	18:30	arg1	cellulose-poly					32:45	hydroxypropyl cellulose-poly	18:45	the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug	14:139	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	0	84	theme	hydroxypropyl	18:30	arg1	alcohol					53:59	vinyl alcohol	47:59	vinyl alcohol	47:59	Prediction of the hydroxypropyl cellulose-poly(vinyl alcohol) ratio in aqueous solution containing papaverine hydrochloride in terms of drug loaded electrospun fiber formation.
28259094	7	85	dep	transform	1167:1175	arg1	infrared					1177:1184	infrared	1177:1184	transform infrared spectroscopy	1167:1197	The crystalline-amorphous transition of papaverine hydrochloride was also tracked by Fourier transform infrared spectroscopy.
28259094	6	86	theme	reflectance	917:927	arg1	characterization					929:944	molar reflectance characterization	911:944	molar reflectance characterization	911:944	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	2	87	theme	soluble	468:474	arg1	HPC					501:503	HPC	501:503	HPC	501:503	The water soluble hydroxypropyl cellulose (HPC) was chosen as a mucoadhesive polymer.
28259094	2	87	theme	soluble	468:474	arg1	cellulose					490:498	The water soluble hydroxypropyl cellulose	458:498	The water soluble hydroxypropyl cellulose (HPC)	458:504	The water soluble hydroxypropyl cellulose (HPC) was chosen as a mucoadhesive polymer.
28259094	2	87	theme	soluble	468:474	arg1	polymer					535:541	a mucoadhesive polymer	520:541	a mucoadhesive polymer	520:541	The water soluble hydroxypropyl cellulose (HPC) was chosen as a mucoadhesive polymer.
28259094	11	88	theme	Polymer	1628:1634	arg1	solutions					1636:1644	Polymer solutions	1628:1644	Polymer solutions of the lowest elasticity and smallest intermolecular interactions	1628:1710	Polymer solutions of the lowest elasticity and smallest intermolecular interactions contributed to the best fiber characteristics of the samples.
28259094	6	89	theme	composition	1029:1039	arg1	determination					1000:1012	the determination	996:1012	the determination of the optimum composition of the gels for fiber formation	996:1071	Combination of rheological measurements and molar reflectance characterization with scanning electron microscopy was applied for the determination of the optimum composition of the gels for fiber formation.
28259094	1	90	theme	drug	337:340	arg1	bioavailability					302:316	the oral bioavailability	293:316	the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism	293:455	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	12	91	theme	polymer	1818:1824	arg1	ratio					1826:1830	the polymer ratio	1814:1830	the polymer ratio for the formation of applicable quality of electrospun fibers	1814:1892	The results enable the determination of the polymer ratio for the formation of applicable quality of electrospun fibers.
28259094	11	92	theme	lowest	1653:1658	arg1	elasticity					1660:1669	the lowest elasticity	1649:1669	the lowest elasticity	1649:1669	Polymer solutions of the lowest elasticity and smallest intermolecular interactions contributed to the best fiber characteristics of the samples.
28259094	8	93	theme	samples	1365:1371	arg1	properties					1252:1261	the macrostructural properties	1232:1261	the macrostructural properties of the polymer solutions and their electrospinnability	1232:1316	A correlation was found between the macrostructural properties of the polymer solutions and their electrospinnability and the consequent morphology of the resultant samples.
28259094	8	93	theme	samples	1365:1371	arg1	morphology					1337:1346	the consequent morphology	1322:1346	the consequent morphology of the resultant samples	1322:1371	A correlation was found between the macrostructural properties of the polymer solutions and their electrospinnability and the consequent morphology of the resultant samples.
28259094	1	94	theme	buccal	246:251	arg1	delivery					258:265	buccal drug delivery	246:265	buccal drug delivery	246:265	Papaverine hydrochloride loaded electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism.
28259094	13	95	theme	best	1959:1962	arg1	performance					1970:1980	the best fiber performance	1955:1980	the best fiber performance	1955:1980	According to the results 5:5 and 6:4 polymer ratios enabled the best fiber performance.
26133314	5	0	theme	fluorescence	862:873	arg1	FRET					902:905	FRET	902:905	FRET	902:905	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	5	0	theme	fluorescence	862:873	arg1	transfer					892:899	fluorescence resonance energy transfer	862:899	the fluorescence resonance energy transfer (FRET) effect	858:913	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	6	1	dep	In	1044:1045	arg1	vitro					1047:1051	vitro	1047:1051	vitro	1047:1051	In vitro study showed the fluorescent nanoparticles could be efficiently internalized by cancer cells and then disrupted by endogenous H2 O2 , accompanying with FRET from "on" to "off".
26133314	6	2	dep	nanoparticles	1082:1094	arg1	internalized					1117:1128	internalized	1117:1128	efficiently internalized by cancer cells	1105:1144	In vitro study showed the fluorescent nanoparticles could be efficiently internalized by cancer cells and then disrupted by endogenous H2 O2 , accompanying with FRET from "on" to "off".
26133314	6	2	dep	nanoparticles	1082:1094	arg1	disrupted					1155:1163	disrupted	1155:1163	then disrupted by endogenous H2 O2	1150:1183	In vitro study showed the fluorescent nanoparticles could be efficiently internalized by cancer cells and then disrupted by endogenous H2 O2 , accompanying with FRET from "on" to "off".
26133314	7	3	theme	multiple	1293:1300	arg1	interactions					1315:1326	multiple non-covalent interactions	1293:1326	multiple non-covalent interactions	1293:1326	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions are expected to have potential applications in diagnosis and imaging of diseases caused by oxidative stresses.
26133314	4	4	theme	self-assembled	649:662	arg1	nanoparticles					664:676	The self-assembled nanoparticles	645:676	The self-assembled nanoparticles based on a combination of multiple non-covalent interactions in aqueous medium	645:755	The self-assembled nanoparticles based on a combination of multiple non-covalent interactions in aqueous medium showed high sensitivity to H2 O2 while maintaining stability under physiological condition.
26133314	3	5	theme	supramolecular	350:363	arg1	nanoparticles					377:389	supramolecular fluorescent nanoparticles	350:389	supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	350:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	5	6	theme	energy	885:890	arg1	FRET					902:905	FRET	902:905	FRET	902:905	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	5	6	theme	energy	885:890	arg1	transfer					892:899	fluorescence resonance energy transfer	862:899	the fluorescence resonance energy transfer (FRET) effect	858:913	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	7	7	theme	non-covalent	1302:1313	arg1	interactions					1315:1326	multiple non-covalent interactions	1293:1326	multiple non-covalent interactions	1293:1326	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions are expected to have potential applications in diagnosis and imaging of diseases caused by oxidative stresses.
26133314	0	8	theme	Peroxide	122:129	arg1	Detection					100:108	the Detection	96:108	the Detection of Hydrogen Peroxide in Cancer Cells	96:145	Supramolecular Fluorescent Nanoparticles Constructed via Multiple Non-Covalent Interactions for the Detection of Hydrogen Peroxide in Cancer Cells.
26133314	7	9	theme	diseases	1400:1407	arg1	diagnosis					1375:1383	diagnosis	1375:1383	diagnosis	1375:1383	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions are expected to have potential applications in diagnosis and imaging of diseases caused by oxidative stresses.
26133314	7	9	theme	diseases	1400:1407	arg1	imaging					1389:1395	imaging	1389:1395	imaging	1389:1395	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions are expected to have potential applications in diagnosis and imaging of diseases caused by oxidative stresses.
26133314	3	10	theme	FITC-β-CD	463:471	arg1	amphiphile					513:522	modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	438:522	fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	411:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	7	11	theme	fluorescent	1251:1261	arg1	nanoparticles					1263:1275	These supramolecular fluorescent nanoparticles	1230:1275	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions	1230:1326	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions are expected to have potential applications in diagnosis and imaging of diseases caused by oxidative stresses.
26133314	3	12	from	detection	618:626	arg1	cells					638:642	cancer cells	631:642	cancer cells	631:642	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	0	13	theme	Hydrogen	113:120	arg1	Peroxide					122:129	Hydrogen Peroxide	113:129	Hydrogen Peroxide	113:129	Supramolecular Fluorescent Nanoparticles Constructed via Multiple Non-Covalent Interactions for the Detection of Hydrogen Peroxide in Cancer Cells.
26133314	5	14	theme	fluorescent	1019:1029	arg1	experiments					1031:1041	fluorescent experiments	1019:1041	fluorescent experiments	1019:1041	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	2	15	theme	H2	322:323	arg1	important					331:339	important	331:339	important	331:339	Thus, the highly sensitive detection of H2 O2 is important.
26133314	2	15	theme	H2	322:323	arg1	detection					309:317	the highly sensitive detection	288:317	the highly sensitive detection of H2 O2	288:326	Thus, the highly sensitive detection of H2 O2 is important.
26133314	6	16	theme	In	1044:1045	arg1	study					1053:1057	In vitro study	1044:1057	In vitro study	1044:1057	In vitro study showed the fluorescent nanoparticles could be efficiently internalized by cancer cells and then disrupted by endogenous H2 O2 , accompanying with FRET from "on" to "off".
26133314	0	17	theme	Cancer	134:139	arg1	Cells					141:145	Cancer Cells	134:145	Cancer Cells	134:145	Supramolecular Fluorescent Nanoparticles Constructed via Multiple Non-Covalent Interactions for the Detection of Hydrogen Peroxide in Cancer Cells.
26133314	5	18	theme	H2	928:929	arg1	addition					916:923	addition	916:923	addition of H2 O2	916:932	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	4	19	theme	multiple	704:711	arg1	interactions					726:737	multiple non-covalent interactions	704:737	multiple non-covalent interactions in aqueous medium	704:755	The self-assembled nanoparticles based on a combination of multiple non-covalent interactions in aqueous medium showed high sensitivity to H2 O2 while maintaining stability under physiological condition.
26133314	4	20	theme	high	764:767	arg1	sensitivity					769:779	high sensitivity	764:779	high sensitivity to H2 O2	764:788	The self-assembled nanoparticles based on a combination of multiple non-covalent interactions in aqueous medium showed high sensitivity to H2 O2 while maintaining stability under physiological condition.
26133314	1	21	theme	environmental	200:212	arg1	stress					214:219	environmental stress	200:219	environmental stress	200:219	Overabundance of hydrogen peroxide originating from environmental stress and/or genetic mutation can lead to pathological conditions.
26133314	3	22	theme	/rhodamine	473:482	arg1	amphiphile					513:522	modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	438:522	fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	411:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	0	23	theme	Multiple	57:64	arg1	Interactions					79:90	Multiple Non-Covalent Interactions	57:90	Multiple Non-Covalent Interactions for the Detection of Hydrogen Peroxide in Cancer Cells	57:145	Supramolecular Fluorescent Nanoparticles Constructed via Multiple Non-Covalent Interactions for the Detection of Hydrogen Peroxide in Cancer Cells.
26133314	3	24	theme	H2	612:613	arg1	detection					618:626	H2 O2 detection	612:626	H2 O2 detection in cancer cells	612:642	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	4	25	from	medium	750:755	arg1	combination					689:699	a combination	687:699	a combination of multiple non-covalent interactions in aqueous medium	687:755	The self-assembled nanoparticles based on a combination of multiple non-covalent interactions in aqueous medium showed high sensitivity to H2 O2 while maintaining stability under physiological condition.
26133314	4	26	theme	aqueous	742:748	arg1	medium					750:755	aqueous medium	742:755	aqueous medium	742:755	The self-assembled nanoparticles based on a combination of multiple non-covalent interactions in aqueous medium showed high sensitivity to H2 O2 while maintaining stability under physiological condition.
26133314	0	27	theme	Fluorescent	15:25	arg1	Nanoparticles					27:39	Supramolecular Fluorescent Nanoparticles	0:39	Supramolecular Fluorescent Nanoparticles	0:39	Supramolecular Fluorescent Nanoparticles Constructed via Multiple Non-Covalent Interactions for the Detection of Hydrogen Peroxide in Cancer Cells.
26133314	5	28	theme	resonance	875:883	arg1	FRET					902:905	FRET	902:905	FRET	902:905	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	5	28	theme	resonance	875:883	arg1	transfer					892:899	fluorescence resonance energy transfer	862:899	the fluorescence resonance energy transfer (FRET) effect	858:913	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	7	29	contain	have	1344:1347	arg1	nanoparticles					1263:1275	These supramolecular fluorescent nanoparticles	1230:1275	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions	1230:1326	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions are expected to have potential applications in diagnosis and imaging of diseases caused by oxidative stresses.
26133314	7	29	contain	have	1344:1347	arg2	applications					1359:1370	potential applications	1349:1370	potential applications	1349:1370	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions are expected to have potential applications in diagnosis and imaging of diseases caused by oxidative stresses.
26133314	3	30	theme	ferrocene	495:503	arg1	amphiphile					513:522	modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	438:522	fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	411:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	0	31	theme	Supramolecular	0:13	arg1	Nanoparticles					27:39	Supramolecular Fluorescent Nanoparticles	0:39	Supramolecular Fluorescent Nanoparticles	0:39	Supramolecular Fluorescent Nanoparticles Constructed via Multiple Non-Covalent Interactions for the Detection of Hydrogen Peroxide in Cancer Cells.
26133314	4	32	theme	physiological	824:836	arg1	condition					838:846	physiological condition	824:846	physiological condition	824:846	The self-assembled nanoparticles based on a combination of multiple non-covalent interactions in aqueous medium showed high sensitivity to H2 O2 while maintaining stability under physiological condition.
26133314	1	33	theme	genetic	228:234	arg1	mutation					236:243	genetic mutation	228:243	genetic mutation	228:243	Overabundance of hydrogen peroxide originating from environmental stress and/or genetic mutation can lead to pathological conditions.
26133314	3	34	theme	modified	438:445	arg1	amphiphile					513:522	modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	438:522	fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	411:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	4	35	from	interactions	726:737	arg1	medium					750:755	aqueous medium	742:755	aqueous medium	742:755	The self-assembled nanoparticles based on a combination of multiple non-covalent interactions in aqueous medium showed high sensitivity to H2 O2 while maintaining stability under physiological condition.
26133314	4	36	theme	non-covalent	713:724	arg1	interactions					726:737	multiple non-covalent interactions	704:737	multiple non-covalent interactions in aqueous medium	704:755	The self-assembled nanoparticles based on a combination of multiple non-covalent interactions in aqueous medium showed high sensitivity to H2 O2 while maintaining stability under physiological condition.
26133314	3	37	theme	β-cyclodextrin	447:460	arg1	amphiphile					513:522	modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	438:522	fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	411:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	6	38	dep	showed	1059:1064	arg1	accompanying					1187:1198	accompanying	1187:1198	accompanying with FRET from "on" to "off"	1187:1227	In vitro study showed the fluorescent nanoparticles could be efficiently internalized by cancer cells and then disrupted by endogenous H2 O2 , accompanying with FRET from "on" to "off".
26133314	3	39	theme	cancer	631:636	arg1	cells					638:642	cancer cells	631:642	cancer cells	631:642	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	1	40	theme	hydrogen	165:172	arg1	peroxide					174:181	hydrogen peroxide	165:181	hydrogen peroxide	165:181	Overabundance of hydrogen peroxide originating from environmental stress and/or genetic mutation can lead to pathological conditions.
26133314	3	41	theme	B	484:484	arg1	amphiphile					513:522	modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	438:522	fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	411:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	7	42	theme	potential	1349:1357	arg1	applications					1359:1370	potential applications	1349:1370	potential applications	1349:1370	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions are expected to have potential applications in diagnosis and imaging of diseases caused by oxidative stresses.
26133314	0	43	from	Detection	100:108	arg1	Cells					141:145	Cancer Cells	134:145	Cancer Cells	134:145	Supramolecular Fluorescent Nanoparticles Constructed via Multiple Non-Covalent Interactions for the Detection of Hydrogen Peroxide in Cancer Cells.
26133314	6	44	theme	cancer	1133:1138	arg1	cells					1140:1144	cancer cells	1133:1144	cancer cells	1133:1144	In vitro study showed the fluorescent nanoparticles could be efficiently internalized by cancer cells and then disrupted by endogenous H2 O2 , accompanying with FRET from "on" to "off".
26133314	5	45	theme	fluorescence	949:960	arg1	change					962:967	obvious fluorescence change	941:967	obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments	941:1041	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	3	46	theme	Fc-RB	506:510	arg1	amphiphile					513:522	modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	438:522	fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	411:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	3	47	theme	modified	486:493	arg1	amphiphile					513:522	modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	438:522	fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	411:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	4	48	theme	interactions	726:737	arg1	combination					689:699	a combination	687:699	a combination of multiple non-covalent interactions in aqueous medium	687:755	The self-assembled nanoparticles based on a combination of multiple non-covalent interactions in aqueous medium showed high sensitivity to H2 O2 while maintaining stability under physiological condition.
26133314	3	49	theme	fluorescein	411:421	arg1	isothiocyanate					423:436	fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	411:522	fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	411:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	5	50	dep	red	991:993	arg1	FITC					1010:1013	FITC	1010:1013	FITC	1010:1013	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	5	50	dep	red	991:993	arg1	RB					996:997	RB	996:997	RB	996:997	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	5	50	dep	red	991:993	arg1	green					1003:1007	green	1003:1007	green	1003:1007	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	5	50	dep	red	991:993	arg1	to					1000:1001	to	1000:1001	to	1000:1001	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	0	51	theme	Non-Covalent	66:77	arg1	Interactions					79:90	Multiple Non-Covalent Interactions	57:90	Multiple Non-Covalent Interactions for the Detection of Hydrogen Peroxide in Cancer Cells	57:145	Supramolecular Fluorescent Nanoparticles Constructed via Multiple Non-Covalent Interactions for the Detection of Hydrogen Peroxide in Cancer Cells.
26133314	3	52	theme	FITC-β-CD	577:585	arg1	host					587:590	FITC-β-CD host	577:590	FITC-β-CD host	577:590	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	7	53	theme	oxidative	1419:1427	arg1	stresses					1429:1436	oxidative stresses	1419:1436	oxidative stresses	1419:1436	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions are expected to have potential applications in diagnosis and imaging of diseases caused by oxidative stresses.
26133314	2	54	theme	sensitive	299:307	arg1	important					331:339	important	331:339	important	331:339	Thus, the highly sensitive detection of H2 O2 is important.
26133314	2	54	theme	sensitive	299:307	arg1	detection					309:317	the highly sensitive detection	288:317	the highly sensitive detection of H2 O2	288:326	Thus, the highly sensitive detection of H2 O2 is important.
26133314	5	55	from	red	991:993	arg1	nanoparticles					972:984	nanoparticles	972:984	nanoparticles from red (RB) to green (FITC)	972:1014	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	5	55	from	red	991:993	arg1	change					962:967	obvious fluorescence change	941:967	obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments	941:1041	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	3	56	theme	host-guest	546:555	arg1	interaction					557:567	host-guest interaction	546:567	host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells	546:642	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	1	57	theme	pathological	257:268	arg1	conditions					270:279	pathological conditions	257:279	pathological conditions	257:279	Overabundance of hydrogen peroxide originating from environmental stress and/or genetic mutation can lead to pathological conditions.
26133314	3	58	dep	isothiocyanate	423:436	arg1	amphiphile					513:522	modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	438:522	fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	411:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	5	59	theme	nanoparticles	972:984	arg1	change					962:967	obvious fluorescence change	941:967	obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments	941:1041	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	3	60	theme	fluorescent	365:375	arg1	nanoparticles					377:389	supramolecular fluorescent nanoparticles	350:389	supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile	350:522	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	6	61	theme	endogenous	1168:1177	arg1	H2					1179:1180	endogenous H2 O2	1168:1183	endogenous H2 O2	1168:1183	In vitro study showed the fluorescent nanoparticles could be efficiently internalized by cancer cells and then disrupted by endogenous H2 O2 , accompanying with FRET from "on" to "off".
26133314	5	62	from	change	962:967	arg1	red					991:993	red	991:993	red	991:993	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	5	62	from	change	962:967	arg1	experiments					1031:1041	fluorescent experiments	1019:1041	fluorescent experiments	1019:1041	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	3	63	theme	Fc-RB	596:600	arg1	guest					602:606	Fc-RB guest	596:606	Fc-RB guest	596:606	Herein, supramolecular fluorescent nanoparticles self-assembled from fluorescein isothiocyanate modified β-cyclodextrin (FITC-β-CD)/rhodamine B modified ferrocene (Fc-RB) amphiphile were prepared through host-guest interaction between FITC-β-CD host and Fc-RB guest for H2 O2 detection in cancer cells.
26133314	5	64	theme	obvious	941:947	arg1	change					962:967	obvious fluorescence change	941:967	obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments	941:1041	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	6	65	theme	fluorescent	1070:1080	arg1	nanoparticles					1082:1094	the fluorescent nanoparticles	1066:1094	the fluorescent nanoparticles could be efficiently internalized by cancer cells and then disrupted by endogenous H2 O2	1066:1183	In vitro study showed the fluorescent nanoparticles could be efficiently internalized by cancer cells and then disrupted by endogenous H2 O2 , accompanying with FRET from "on" to "off".
26133314	4	66	from	combination	689:699	arg1	medium					750:755	aqueous medium	742:755	aqueous medium	742:755	The self-assembled nanoparticles based on a combination of multiple non-covalent interactions in aqueous medium showed high sensitivity to H2 O2 while maintaining stability under physiological condition.
26133314	5	67	theme	transfer	892:899	arg1	effect					908:913	the fluorescence resonance energy transfer (FRET) effect	858:913	the fluorescence resonance energy transfer (FRET) effect	858:913	Owing to the fluorescence resonance energy transfer (FRET) effect, addition of H2 O2 led to obvious fluorescence change of nanoparticles from red (RB) to green (FITC) in fluorescent experiments.
26133314	1	68	theme	peroxide	174:181	arg1	Overabundance					148:160	Overabundance	148:160	Overabundance of hydrogen peroxide originating from environmental stress and/or genetic mutation	148:243	Overabundance of hydrogen peroxide originating from environmental stress and/or genetic mutation can lead to pathological conditions.
26133314	7	69	theme	supramolecular	1236:1249	arg1	nanoparticles					1263:1275	These supramolecular fluorescent nanoparticles	1230:1275	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions	1230:1326	These supramolecular fluorescent nanoparticles constructed via multiple non-covalent interactions are expected to have potential applications in diagnosis and imaging of diseases caused by oxidative stresses.
27716628	10	0	theme	high	1686:1689	arg1	viability					1696:1704	high cell viability	1686:1704	high cell viability	1686:1704	Finally we demonstrate that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments, generating composites with bulk compressive moduli comparable to articular cartilage.
27716628	2	1	theme	mechanical	329:338	arg1	integrity					340:348	the mechanical integrity	325:348	the mechanical integrity for implantation into high load bearing environments	325:401	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	4	2	theme	used	593:596	arg1	bioinks					607:613	commonly used hydrogel bioinks	584:613	commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro	584:777	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	10	3	theme	hydrogel	1725:1732	arg1	bioink					1734:1739	a hydrogel bioink	1723:1739	a hydrogel bioink with polycaprolactone filaments	1723:1771	Finally we demonstrate that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments, generating composites with bulk compressive moduli comparable to articular cartilage.
27716628	2	4	dep	fibrocartilage	238:251	arg1	engineering					284:294	tissue engineering	277:294	tissue engineering	277:294	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	7	5	theme	type	1361:1364	arg1	collagen					1369:1376	type II collagen	1361:1376	type II collagen	1361:1376	In contrast, GelMA and BioINK™ (a PEGMA based hydrogel) supported the development of a more fibrocartilage-like tissue, as evident by the development of a tissue containing both type I and type II collagen.
27716628	2	6	used	used	229:232	arg2	hydrogels					206:214	Mesenchymal stem cell (MSC) laden hydrogels	172:214	Mesenchymal stem cell (MSC) laden hydrogels	172:214	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	11	7	theme	musculoskeletal	1976:1990	arg1	applications					1992:2003	musculoskeletal applications	1976:2003	musculoskeletal applications	1976:2003	This study demonstrates the importance of the choice of bioink when bioprinting different cartilaginous tissues for musculoskeletal applications.
27716628	4	8	dep	agarose	616:622	arg1	BioINK™					645:651	BioINK™	645:651	BioINK™	645:651	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	4	8	dep	agarose	616:622	arg1	GelMA					635:639	GelMA	635:639	GelMA	635:639	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	4	8	dep	agarose	616:622	arg1	alginate					625:632	alginate	625:632	alginate	625:632	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	1	9	theme	dense	109:113	arg1	tissue					126:131	a dense connective tissue	107:131	a dense connective tissue with limited self-repair capabilities	107:169	Cartilage is a dense connective tissue with limited self-repair capabilities.
27716628	1	9	theme	dense	109:113	arg1	Cartilage					94:102	Cartilage	94:102	Cartilage	94:102	Cartilage is a dense connective tissue with limited self-repair capabilities.
27716628	8	10	theme	greater	1448:1454	arg1	fidelity					1456:1463	greater fidelity	1448:1463	greater fidelity	1448:1463	GelMA demonstrated superior printability, generating structures with greater fidelity, followed by the alginate and agarose bioinks.
27716628	7	11	theme	tissue	1327:1332	arg1	development					1310:1320	the development	1306:1320	the development of a tissue containing both type I and type II collagen	1306:1376	In contrast, GelMA and BioINK™ (a PEGMA based hydrogel) supported the development of a more fibrocartilage-like tissue, as evident by the development of a tissue containing both type I and type II collagen.
27716628	4	12	dep	bioinks	607:613	arg1	agarose					616:622	agarose	616:622	agarose	616:622	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	10	13	theme	comparable	1825:1834	arg1	moduli					1818:1823	bulk compressive moduli	1801:1823	bulk compressive moduli comparable to articular cartilage	1801:1857	Finally we demonstrate that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments, generating composites with bulk compressive moduli comparable to articular cartilage.
27716628	5	14	theme	indicative	893:902	arg1	markers					885:891	markers	885:891	markers indicative of differentiation towards either a fibrocartilaginous or hyaline cartilage-like phenotype	885:993	Each hydrogel was seeded with MSCs, cultured for 28 days in the presence of TGF-β3 and then analysed for markers indicative of differentiation towards either a fibrocartilaginous or hyaline cartilage-like phenotype.
27716628	4	15	theme	printing	664:671	arg1	properties					673:682	their printing properties	658:682	their printing properties	658:682	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	7	16	theme	based	1212:1216	arg1	hydrogel					1218:1225	a PEGMA based hydrogel	1204:1225	a PEGMA based hydrogel	1204:1225	In contrast, GelMA and BioINK™ (a PEGMA based hydrogel) supported the development of a more fibrocartilage-like tissue, as evident by the development of a tissue containing both type I and type II collagen.
27716628	7	16	theme	based	1212:1216	arg1	GelMA					1185:1189	GelMA	1185:1189	GelMA	1185:1189	In contrast, GelMA and BioINK™ (a PEGMA based hydrogel) supported the development of a more fibrocartilage-like tissue, as evident by the development of a tissue containing both type I and type II collagen.
27716628	10	17	theme	compressive	1806:1816	arg1	moduli					1818:1823	bulk compressive moduli	1801:1823	bulk compressive moduli comparable to articular cartilage	1801:1857	Finally we demonstrate that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments, generating composites with bulk compressive moduli comparable to articular cartilage.
27716628	5	18	theme	TGF-β3	856:861	arg1	presence					844:851	the presence	840:851	the presence of TGF-β3	840:861	Each hydrogel was seeded with MSCs, cultured for 28 days in the presence of TGF-β3 and then analysed for markers indicative of differentiation towards either a fibrocartilaginous or hyaline cartilage-like phenotype.
27716628	9	19	theme	High	1512:1515	arg1	levels					1517:1522	High levels	1512:1522	High levels of MSC viability	1512:1539	High levels of MSC viability were observed in all bioinks post-printing (∼80%).
27716628	9	20	located	observed	1546:1553	arg2	levels					1517:1522	High levels	1512:1522	High levels of MSC viability	1512:1539	High levels of MSC viability were observed in all bioinks post-printing (∼80%).
27716628	9	20	located	observed	1546:1553	arg1	bioinks					1562:1568	all bioinks	1558:1568	all bioinks post-printing (∼80%)	1558:1589	High levels of MSC viability were observed in all bioinks post-printing (∼80%).
27716628	8	21	theme	alginate	1482:1489	arg1	bioinks					1503:1509	the alginate and agarose bioinks	1478:1509	bioinks	1503:1509	GelMA demonstrated superior printability, generating structures with greater fidelity, followed by the alginate and agarose bioinks.
27716628	0	22	theme	cartilage	83:91	arg1	bioprinting					41:51	3D bioprinting	38:51	3D bioprinting of fibrocartilage and hyaline cartilage	38:91	A comparison of different bioinks for 3D bioprinting of fibrocartilage and hyaline cartilage.
27716628	5	23	theme	fibrocartilaginous	940:957	arg1	phenotype					985:993	either a fibrocartilaginous or hyaline cartilage-like phenotype	931:993	phenotype	985:993	Each hydrogel was seeded with MSCs, cultured for 28 days in the presence of TGF-β3 and then analysed for markers indicative of differentiation towards either a fibrocartilaginous or hyaline cartilage-like phenotype.
27716628	8	24	theme	agarose	1495:1501	arg1	bioinks					1503:1509	the alginate and agarose bioinks	1478:1509	bioinks	1503:1509	GelMA demonstrated superior printability, generating structures with greater fidelity, followed by the alginate and agarose bioinks.
27716628	3	25	theme	hydrogel	473:480	arg1	bioinks					482:488	cell laden hydrogel bioinks	462:488	cell laden hydrogel bioinks reinforced with stiffer polymer fibres	462:527	This has led to increased interested in 3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres.
27716628	3	26	theme	3D	444:445	arg1	bioprinting					447:457	3D bioprinting	444:457	3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres	444:527	This has led to increased interested in 3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres.
27716628	1	27	with	tissue	126:131	arg1	capabilities					158:169	limited self-repair capabilities	138:169	limited self-repair capabilities	138:169	Cartilage is a dense connective tissue with limited self-repair capabilities.
27716628	9	28	theme	viability	1531:1539	arg1	levels					1517:1522	High levels	1512:1522	High levels of MSC viability	1512:1539	High levels of MSC viability were observed in all bioinks post-printing (∼80%).
27716628	0	29	theme	fibrocartilage	56:69	arg1	bioprinting					41:51	3D bioprinting	38:51	3D bioprinting of fibrocartilage and hyaline cartilage	38:91	A comparison of different bioinks for 3D bioprinting of fibrocartilage and hyaline cartilage.
27716628	5	30	theme	differentiation	907:921	arg1	indicative					893:902	indicative	893:902	indicative	893:902	Each hydrogel was seeded with MSCs, cultured for 28 days in the presence of TGF-β3 and then analysed for markers indicative of differentiation towards either a fibrocartilaginous or hyaline cartilage-like phenotype.
27716628	6	31	theme	hyaline-like	1061:1072	arg1	cartilage					1074:1082	hyaline-like cartilage	1061:1082	hyaline-like cartilage	1061:1082	Alginate and agarose hydrogels best supported the development of hyaline-like cartilage, as evident by the development of a tissue staining predominantly for type II collagen.
27716628	4	32	theme	hyaline	734:740	arg1	cartilage					742:750	hyaline cartilage	734:750	hyaline cartilage	734:750	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	3	33	theme	laden	467:471	arg1	bioinks					482:488	cell laden hydrogel bioinks	462:488	cell laden hydrogel bioinks reinforced with stiffer polymer fibres	462:527	This has led to increased interested in 3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres.
27716628	2	34	theme	stem	184:187	arg1	MSC					195:197	MSC	195:197	MSC	195:197	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	2	34	theme	stem	184:187	arg1	cell					189:192	Mesenchymal stem cell	172:192	Mesenchymal stem cell (MSC) laden hydrogels	172:214	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	11	35	theme	bioink	1916:1921	arg1	choice					1906:1911	the choice	1902:1911	the choice of bioink	1902:1921	This study demonstrates the importance of the choice of bioink when bioprinting different cartilaginous tissues for musculoskeletal applications.
27716628	0	36	theme	bioinks	26:32	arg1	comparison					2:11	A comparison	0:11	A comparison of different bioinks for 3D bioprinting of fibrocartilage and hyaline cartilage.	0:92	A comparison of different bioinks for 3D bioprinting of fibrocartilage and hyaline cartilage.
27716628	9	37	theme	post-printing	1570:1582	arg1	bioinks					1562:1568	all bioinks	1558:1568	all bioinks post-printing (∼80%)	1558:1589	High levels of MSC viability were observed in all bioinks post-printing (∼80%).
27716628	7	38	theme	tissue	1284:1289	arg1	development					1242:1252	the development	1238:1252	the development of a more fibrocartilage-like tissue	1238:1289	In contrast, GelMA and BioINK™ (a PEGMA based hydrogel) supported the development of a more fibrocartilage-like tissue, as evident by the development of a tissue containing both type I and type II collagen.
27716628	10	39	theme	articular	1839:1847	arg1	cartilage					1849:1857	articular cartilage	1839:1857	articular cartilage	1839:1857	Finally we demonstrate that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments, generating composites with bulk compressive moduli comparable to articular cartilage.
27716628	0	40	theme	3D	38:39	arg1	bioprinting					41:51	3D bioprinting	38:51	3D bioprinting of fibrocartilage and hyaline cartilage	38:91	A comparison of different bioinks for 3D bioprinting of fibrocartilage and hyaline cartilage.
27716628	6	41	theme	tissue	1120:1125	arg1	development					1103:1113	the development	1099:1113	the development of a tissue staining predominantly for type II collagen	1099:1169	Alginate and agarose hydrogels best supported the development of hyaline-like cartilage, as evident by the development of a tissue staining predominantly for type II collagen.
27716628	10	42	theme	reinforced	1660:1669	arg1	hydrogels					1671:1679	mechanically reinforced hydrogels	1647:1679	mechanically reinforced hydrogels with high cell viability	1647:1704	Finally we demonstrate that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments, generating composites with bulk compressive moduli comparable to articular cartilage.
27716628	1	43	theme	limited	138:144	arg1	capabilities					158:169	limited self-repair capabilities	138:169	limited self-repair capabilities	138:169	Cartilage is a dense connective tissue with limited self-repair capabilities.
27716628	4	44	theme	hydrogel	598:605	arg1	bioinks					607:613	commonly used hydrogel bioinks	584:613	commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro	584:777	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	2	45	theme	load	377:380	arg1	environments					390:401	high load bearing environments	372:401	high load bearing environments	372:401	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	7	46	contain	containing	1334:1343	arg1	tissue					1327:1332	a tissue	1325:1332	a tissue containing both type I and type II collagen	1325:1376	In contrast, GelMA and BioINK™ (a PEGMA based hydrogel) supported the development of a more fibrocartilage-like tissue, as evident by the development of a tissue containing both type I and type II collagen.
27716628	7	46	contain	containing	1334:1343	arg2	type					1350:1353	type I	1350:1355	type I	1350:1355	In contrast, GelMA and BioINK™ (a PEGMA based hydrogel) supported the development of a more fibrocartilage-like tissue, as evident by the development of a tissue containing both type I and type II collagen.
27716628	7	46	contain	containing	1334:1343	arg2	collagen					1369:1376	type II collagen	1361:1376	type II collagen	1361:1376	In contrast, GelMA and BioINK™ (a PEGMA based hydrogel) supported the development of a more fibrocartilage-like tissue, as evident by the development of a tissue containing both type I and type II collagen.
27716628	3	47	from	bioprinting	447:457	arg1	interested					430:439	interested	430:439	interested	430:439	This has led to increased interested in 3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres.
27716628	3	48	theme	polymer	514:520	arg1	fibres					522:527	stiffer polymer fibres	506:527	stiffer polymer fibres	506:527	This has led to increased interested in 3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres.
27716628	2	49	theme	tissue	277:282	arg1	engineering					284:294	tissue engineering	277:294	tissue engineering	277:294	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	6	50	theme	type	1154:1157	arg1	collagen					1162:1169	type II collagen	1154:1169	type II collagen	1154:1169	Alginate and agarose hydrogels best supported the development of hyaline-like cartilage, as evident by the development of a tissue staining predominantly for type II collagen.
27716628	9	51	dep	post-printing	1570:1582	arg1	%					1588:1588	∼80%	1585:1588	∼80%	1585:1588	High levels of MSC viability were observed in all bioinks post-printing (∼80%).
27716628	10	52	theme	polycaprolactone	1746:1761	arg1	filaments					1763:1771	polycaprolactone filaments	1746:1771	polycaprolactone filaments	1746:1771	Finally we demonstrate that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments, generating composites with bulk compressive moduli comparable to articular cartilage.
27716628	10	53	theme	bulk	1801:1804	arg1	moduli					1818:1823	bulk compressive moduli	1801:1823	bulk compressive moduli comparable to articular cartilage	1801:1857	Finally we demonstrate that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments, generating composites with bulk compressive moduli comparable to articular cartilage.
27716628	5	54	dep	phenotype	985:993	arg1	either					931:936	either	931:936	either	931:936	Each hydrogel was seeded with MSCs, cultured for 28 days in the presence of TGF-β3 and then analysed for markers indicative of differentiation towards either a fibrocartilaginous or hyaline cartilage-like phenotype.
27716628	11	55	theme	cartilaginous	1950:1962	arg1	tissues					1964:1970	different cartilaginous tissues	1940:1970	different cartilaginous tissues	1940:1970	This study demonstrates the importance of the choice of bioink when bioprinting different cartilaginous tissues for musculoskeletal applications.
27716628	2	56	theme	articular	257:265	arg1	cartilage					267:275	articular cartilage	257:275	articular cartilage	257:275	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	1	57	theme	connective	115:124	arg1	tissue					126:131	a dense connective tissue	107:131	a dense connective tissue with limited self-repair capabilities	107:169	Cartilage is a dense connective tissue with limited self-repair capabilities.
27716628	1	57	theme	connective	115:124	arg1	Cartilage					94:102	Cartilage	94:102	Cartilage	94:102	Cartilage is a dense connective tissue with limited self-repair capabilities.
27716628	3	58	theme	bioinks	482:488	arg1	bioprinting					447:457	3D bioprinting	444:457	3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres	444:527	This has led to increased interested in 3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres.
27716628	7	59	theme	PEGMA	1206:1210	arg1	hydrogel					1218:1225	a PEGMA based hydrogel	1204:1225	a PEGMA based hydrogel	1204:1225	In contrast, GelMA and BioINK™ (a PEGMA based hydrogel) supported the development of a more fibrocartilage-like tissue, as evident by the development of a tissue containing both type I and type II collagen.
27716628	7	59	theme	PEGMA	1206:1210	arg1	GelMA					1185:1189	GelMA	1185:1189	GelMA	1185:1189	In contrast, GelMA and BioINK™ (a PEGMA based hydrogel) supported the development of a more fibrocartilage-like tissue, as evident by the development of a tissue containing both type I and type II collagen.
27716628	10	60	with	bioink	1734:1739	arg1	filaments					1763:1771	polycaprolactone filaments	1746:1771	polycaprolactone filaments	1746:1771	Finally we demonstrate that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments, generating composites with bulk compressive moduli comparable to articular cartilage.
27716628	0	61	theme	hyaline	75:81	arg1	cartilage					83:91	hyaline cartilage	75:91	hyaline cartilage	75:91	A comparison of different bioinks for 3D bioprinting of fibrocartilage and hyaline cartilage.
27716628	6	62	theme	agarose	1009:1015	arg1	hydrogels					1017:1025	agarose hydrogels	1009:1025	agarose hydrogels	1009:1025	Alginate and agarose hydrogels best supported the development of hyaline-like cartilage, as evident by the development of a tissue staining predominantly for type II collagen.
27716628	1	63	theme	self-repair	146:156	arg1	capabilities					158:169	limited self-repair capabilities	138:169	limited self-repair capabilities	138:169	Cartilage is a dense connective tissue with limited self-repair capabilities.
27716628	4	64	theme	cartilage	742:750	arg1	development					712:722	the development	708:722	the development of either hyaline cartilage or fibrocartilage	708:768	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	9	65	theme	MSC	1527:1529	arg1	viability					1531:1539	MSC viability	1527:1539	MSC viability	1527:1539	High levels of MSC viability were observed in all bioinks post-printing (∼80%).
27716628	4	66	theme	fibrocartilage	755:768	arg1	development					712:722	the development	708:722	the development of either hyaline cartilage or fibrocartilage	708:768	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	6	67	theme	cartilage	1074:1082	arg1	development					1046:1056	the development	1042:1056	the development of hyaline-like cartilage	1042:1082	Alginate and agarose hydrogels best supported the development of hyaline-like cartilage, as evident by the development of a tissue staining predominantly for type II collagen.
27716628	2	68	theme	bearing	382:388	arg1	environments					390:401	high load bearing environments	372:401	high load bearing environments	372:401	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	0	69	theme	different	16:24	arg1	bioinks					26:32	different bioinks	16:32	different bioinks	16:32	A comparison of different bioinks for 3D bioprinting of fibrocartilage and hyaline cartilage.
27716628	2	70	theme	cell	189:192	arg1	hydrogels					206:214	Mesenchymal stem cell (MSC) laden hydrogels	172:214	Mesenchymal stem cell (MSC) laden hydrogels	172:214	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	11	71	theme	different	1940:1948	arg1	tissues					1964:1970	different cartilaginous tissues	1940:1970	different cartilaginous tissues	1940:1970	This study demonstrates the importance of the choice of bioink when bioprinting different cartilaginous tissues for musculoskeletal applications.
27716628	7	72	theme	fibrocartilage-like	1264:1282	arg1	tissue					1284:1289	a more fibrocartilage-like tissue	1257:1289	a more fibrocartilage-like tissue	1257:1289	In contrast, GelMA and BioINK™ (a PEGMA based hydrogel) supported the development of a more fibrocartilage-like tissue, as evident by the development of a tissue containing both type I and type II collagen.
27716628	11	73	theme	choice	1906:1911	arg1	importance					1888:1897	the importance	1884:1897	the importance of the choice of bioink	1884:1921	This study demonstrates the importance of the choice of bioink when bioprinting different cartilaginous tissues for musculoskeletal applications.
27716628	2	74	theme	Mesenchymal	172:182	arg1	MSC					195:197	MSC	195:197	MSC	195:197	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	2	74	theme	Mesenchymal	172:182	arg1	cell					189:192	Mesenchymal stem cell	172:192	Mesenchymal stem cell (MSC) laden hydrogels	172:214	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	3	75	theme	cell	462:465	arg1	bioinks					482:488	cell laden hydrogel bioinks	462:488	cell laden hydrogel bioinks reinforced with stiffer polymer fibres	462:527	This has led to increased interested in 3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres.
27716628	10	76	with	hydrogels	1671:1679	arg1	viability					1696:1704	high cell viability	1686:1704	high cell viability	1686:1704	Finally we demonstrate that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments, generating composites with bulk compressive moduli comparable to articular cartilage.
27716628	4	77	theme	study	552:556	arg1	objective					534:542	The objective	530:542	The objective of this study	530:556	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	2	78	theme	laden	200:204	arg1	hydrogels					206:214	Mesenchymal stem cell (MSC) laden hydrogels	172:214	Mesenchymal stem cell (MSC) laden hydrogels	172:214	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
27716628	3	79	theme	stiffer	506:512	arg1	fibres					522:527	stiffer polymer fibres	506:527	stiffer polymer fibres	506:527	This has led to increased interested in 3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres.
27716628	5	80	theme	cartilage-like	970:983	arg1	phenotype					985:993	either a fibrocartilaginous or hyaline cartilage-like phenotype	931:993	phenotype	985:993	Each hydrogel was seeded with MSCs, cultured for 28 days in the presence of TGF-β3 and then analysed for markers indicative of differentiation towards either a fibrocartilaginous or hyaline cartilage-like phenotype.
27716628	4	81	theme	bioinks	607:613	arg1	range					575:579	a range	573:579	a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro	573:777	The objective of this study was to compare a range of commonly used hydrogel bioinks (agarose, alginate, GelMA and BioINK™) for their printing properties and capacity to support the development of either hyaline cartilage or fibrocartilage in vitro.
27716628	3	82	from	interested	430:439	arg1	bioprinting					447:457	3D bioprinting	444:457	3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres	444:527	This has led to increased interested in 3D bioprinting of cell laden hydrogel bioinks reinforced with stiffer polymer fibres.
27716628	10	83	theme	cell	1691:1694	arg1	viability					1696:1704	high cell viability	1686:1704	high cell viability	1686:1704	Finally we demonstrate that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments, generating composites with bulk compressive moduli comparable to articular cartilage.
27716628	8	84	theme	superior	1398:1405	arg1	printability					1407:1418	superior printability	1398:1418	superior printability	1398:1418	GelMA demonstrated superior printability, generating structures with greater fidelity, followed by the alginate and agarose bioinks.
27716628	2	85	theme	high	372:375	arg1	environments					390:401	high load bearing environments	372:401	high load bearing environments	372:401	Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage tissue engineering, however they typically lack the mechanical integrity for implantation into high load bearing environments.
26207761	0	0	theme	α-Glucosidase	67:79	arg1	Inhibitors					95:104	α-Glucosidase and α-Amylase Inhibitors	67:104	α-Glucosidase and α-Amylase Inhibitors	67:104	Synthesis and Evaluation of a Series of Oleanolic Acid Saponins as α-Glucosidase and α-Amylase Inhibitors.
26207761	1	1	theme	inhibitory	248:257	arg1	activities					259:268	their inhibitory activities	242:268	their inhibitory activities against α-glucosidase and α-amylase	242:304	Sixteen naturally occurring oleanolic acid saponins and their derivatives were synthesized in an efficient and practical strategy, and their inhibitory activities against α-glucosidase and α-amylase were evaluated in vitro.
26207761	4	2	theme	oleanolic	860:868	arg1	scaffold					875:882	the oleanolic acid scaffold	856:882	the oleanolic acid scaffold	856:882	Furthermore, for 3,28-O-bidesmosides, sugar-substituted moieties attached to the C-3 and C-28 positions of the oleanolic acid scaffold are helpful to increase the inhibitory activities against α-amylase and α-glucosidase.
26207761	0	3	theme	α-Amylase	85:93	arg1	Inhibitors					95:104	α-Glucosidase and α-Amylase Inhibitors	67:104	α-Glucosidase and α-Amylase Inhibitors	67:104	Synthesis and Evaluation of a Series of Oleanolic Acid Saponins as α-Glucosidase and α-Amylase Inhibitors.
26207761	4	4	attach	attached	814:821	arg1	positions					843:851	the C-3 and C-28 positions	826:851	positions	843:851	Furthermore, for 3,28-O-bidesmosides, sugar-substituted moieties attached to the C-3 and C-28 positions of the oleanolic acid scaffold are helpful to increase the inhibitory activities against α-amylase and α-glucosidase.
26207761	4	4	attach	attached	814:821	arg2	moieties					805:812	sugar-substituted moieties	787:812	sugar-substituted moieties attached to the C-3 and C-28 positions of the oleanolic acid scaffold	787:882	Furthermore, for 3,28-O-bidesmosides, sugar-substituted moieties attached to the C-3 and C-28 positions of the oleanolic acid scaffold are helpful to increase the inhibitory activities against α-amylase and α-glucosidase.
26207761	3	5	theme	preliminary	559:569	arg1	relationships					590:602	the preliminary structure-activity relationships	555:602	the preliminary structure-activity relationships	555:602	Based on the preliminary structure-activity relationships, for 28-O-monoglucosides, the presence of a terminal α-l-rhamnopyranosyl residue enhanced the α-glucosidase and α-amylase inhibitory activities.
26207761	4	6	theme	C-28	838:841	arg1	positions					843:851	the C-3 and C-28 positions	826:851	positions	843:851	Furthermore, for 3,28-O-bidesmosides, sugar-substituted moieties attached to the C-3 and C-28 positions of the oleanolic acid scaffold are helpful to increase the inhibitory activities against α-amylase and α-glucosidase.
26207761	3	7	theme	structure-activity	571:588	arg1	relationships					590:602	the preliminary structure-activity relationships	555:602	the preliminary structure-activity relationships	555:602	Based on the preliminary structure-activity relationships, for 28-O-monoglucosides, the presence of a terminal α-l-rhamnopyranosyl residue enhanced the α-glucosidase and α-amylase inhibitory activities.
26207761	2	8	theme	antidiabetic	523:534	arg1	acarbose					536:543	the antidiabetic acarbose	519:543	the antidiabetic acarbose	519:543	Among all the compounds, 28-O-monoglucoside 8 exhibited remarkably potent inhibitory activity against α-glucosidase with an IC50 value of 87.3 µM, which was fivefold stronger than that of the antidiabetic acarbose.
26207761	4	9	theme	inhibitory	912:921	arg1	activities					923:932	the inhibitory activities	908:932	the inhibitory activities against α-amylase and α-glucosidase	908:968	Furthermore, for 3,28-O-bidesmosides, sugar-substituted moieties attached to the C-3 and C-28 positions of the oleanolic acid scaffold are helpful to increase the inhibitory activities against α-amylase and α-glucosidase.
26207761	2	10	theme	inhibitory	405:414	arg1	activity					416:423	remarkably potent inhibitory activity	387:423	remarkably potent inhibitory activity against α-glucosidase	387:445	Among all the compounds, 28-O-monoglucoside 8 exhibited remarkably potent inhibitory activity against α-glucosidase with an IC50 value of 87.3 µM, which was fivefold stronger than that of the antidiabetic acarbose.
26207761	2	11	theme	87.3 µM	469:475	arg1	value					460:464	an IC50 value	452:464	an IC50 value of 87.3 µM, which was fivefold stronger than that of the antidiabetic acarbose	452:543	Among all the compounds, 28-O-monoglucoside 8 exhibited remarkably potent inhibitory activity against α-glucosidase with an IC50 value of 87.3 µM, which was fivefold stronger than that of the antidiabetic acarbose.
26207761	2	12	theme	potent	398:403	arg1	activity					416:423	remarkably potent inhibitory activity	387:423	remarkably potent inhibitory activity against α-glucosidase	387:445	Among all the compounds, 28-O-monoglucoside 8 exhibited remarkably potent inhibitory activity against α-glucosidase with an IC50 value of 87.3 µM, which was fivefold stronger than that of the antidiabetic acarbose.
26207761	2	13	theme	IC50	455:458	arg1	value					460:464	an IC50 value	452:464	an IC50 value of 87.3 µM, which was fivefold stronger than that of the antidiabetic acarbose	452:543	Among all the compounds, 28-O-monoglucoside 8 exhibited remarkably potent inhibitory activity against α-glucosidase with an IC50 value of 87.3 µM, which was fivefold stronger than that of the antidiabetic acarbose.
26207761	3	14	theme	α-glucosidase	698:710	arg1	activities					737:746	the α-glucosidase and α-amylase inhibitory activities	694:746	the α-glucosidase and α-amylase inhibitory activities	694:746	Based on the preliminary structure-activity relationships, for 28-O-monoglucosides, the presence of a terminal α-l-rhamnopyranosyl residue enhanced the α-glucosidase and α-amylase inhibitory activities.
26207761	4	15	theme	C-3	830:832	arg1	positions					843:851	the C-3 and C-28 positions	826:851	positions	843:851	Furthermore, for 3,28-O-bidesmosides, sugar-substituted moieties attached to the C-3 and C-28 positions of the oleanolic acid scaffold are helpful to increase the inhibitory activities against α-amylase and α-glucosidase.
26207761	1	16	theme	efficient	204:212	arg1	strategy					228:235	an efficient and practical strategy	201:235	an efficient and practical strategy	201:235	Sixteen naturally occurring oleanolic acid saponins and their derivatives were synthesized in an efficient and practical strategy, and their inhibitory activities against α-glucosidase and α-amylase were evaluated in vitro.
26207761	0	17	theme	Series	30:35	arg1	Evaluation					14:23	Evaluation	14:23	Evaluation	14:23	Synthesis and Evaluation of a Series of Oleanolic Acid Saponins as α-Glucosidase and α-Amylase Inhibitors.
26207761	0	17	theme	Series	30:35	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and Evaluation of a Series of Oleanolic Acid Saponins as α-Glucosidase and α-Amylase Inhibitors.
26207761	1	18	theme	practical	218:226	arg1	strategy					228:235	an efficient and practical strategy	201:235	an efficient and practical strategy	201:235	Sixteen naturally occurring oleanolic acid saponins and their derivatives were synthesized in an efficient and practical strategy, and their inhibitory activities against α-glucosidase and α-amylase were evaluated in vitro.
26207761	0	19	theme	Oleanolic	40:48	arg1	Saponins					55:62	Oleanolic Acid Saponins	40:62	Oleanolic Acid Saponins as α-Glucosidase and α-Amylase Inhibitors	40:104	Synthesis and Evaluation of a Series of Oleanolic Acid Saponins as α-Glucosidase and α-Amylase Inhibitors.
26207761	4	20	theme	sugar-substituted	787:803	arg1	moieties					805:812	sugar-substituted moieties	787:812	sugar-substituted moieties attached to the C-3 and C-28 positions of the oleanolic acid scaffold	787:882	Furthermore, for 3,28-O-bidesmosides, sugar-substituted moieties attached to the C-3 and C-28 positions of the oleanolic acid scaffold are helpful to increase the inhibitory activities against α-amylase and α-glucosidase.
26207761	4	21	theme	scaffold	875:882	arg1	positions					843:851	the C-3 and C-28 positions	826:851	positions	843:851	Furthermore, for 3,28-O-bidesmosides, sugar-substituted moieties attached to the C-3 and C-28 positions of the oleanolic acid scaffold are helpful to increase the inhibitory activities against α-amylase and α-glucosidase.
26207761	4	22	theme	acid	870:873	arg1	scaffold					875:882	the oleanolic acid scaffold	856:882	the oleanolic acid scaffold	856:882	Furthermore, for 3,28-O-bidesmosides, sugar-substituted moieties attached to the C-3 and C-28 positions of the oleanolic acid scaffold are helpful to increase the inhibitory activities against α-amylase and α-glucosidase.
26207761	3	23	theme	α-l-rhamnopyranosyl	657:675	arg1	residue					677:683	a terminal α-l-rhamnopyranosyl residue	646:683	a terminal α-l-rhamnopyranosyl residue	646:683	Based on the preliminary structure-activity relationships, for 28-O-monoglucosides, the presence of a terminal α-l-rhamnopyranosyl residue enhanced the α-glucosidase and α-amylase inhibitory activities.
26207761	1	24	theme	occurring	125:133	arg1	saponins					150:157	Sixteen naturally occurring oleanolic acid saponins	107:157	Sixteen naturally occurring oleanolic acid saponins	107:157	Sixteen naturally occurring oleanolic acid saponins and their derivatives were synthesized in an efficient and practical strategy, and their inhibitory activities against α-glucosidase and α-amylase were evaluated in vitro.
26207761	3	25	theme	α-amylase	716:724	arg1	activities					737:746	the α-glucosidase and α-amylase inhibitory activities	694:746	the α-glucosidase and α-amylase inhibitory activities	694:746	Based on the preliminary structure-activity relationships, for 28-O-monoglucosides, the presence of a terminal α-l-rhamnopyranosyl residue enhanced the α-glucosidase and α-amylase inhibitory activities.
26207761	3	26	theme	residue	677:683	arg1	presence					634:641	the presence	630:641	the presence of a terminal α-l-rhamnopyranosyl residue	630:683	Based on the preliminary structure-activity relationships, for 28-O-monoglucosides, the presence of a terminal α-l-rhamnopyranosyl residue enhanced the α-glucosidase and α-amylase inhibitory activities.
26207761	1	27	theme	oleanolic	135:143	arg1	saponins					150:157	Sixteen naturally occurring oleanolic acid saponins	107:157	Sixteen naturally occurring oleanolic acid saponins	107:157	Sixteen naturally occurring oleanolic acid saponins and their derivatives were synthesized in an efficient and practical strategy, and their inhibitory activities against α-glucosidase and α-amylase were evaluated in vitro.
26207761	0	28	theme	Saponins	55:62	arg1	Series					30:35	a Series	28:35	a Series of Oleanolic Acid Saponins as α-Glucosidase and α-Amylase Inhibitors	28:104	Synthesis and Evaluation of a Series of Oleanolic Acid Saponins as α-Glucosidase and α-Amylase Inhibitors.
26207761	3	29	theme	inhibitory	726:735	arg1	activities					737:746	the α-glucosidase and α-amylase inhibitory activities	694:746	the α-glucosidase and α-amylase inhibitory activities	694:746	Based on the preliminary structure-activity relationships, for 28-O-monoglucosides, the presence of a terminal α-l-rhamnopyranosyl residue enhanced the α-glucosidase and α-amylase inhibitory activities.
26207761	1	30	theme	acid	145:148	arg1	saponins					150:157	Sixteen naturally occurring oleanolic acid saponins	107:157	Sixteen naturally occurring oleanolic acid saponins	107:157	Sixteen naturally occurring oleanolic acid saponins and their derivatives were synthesized in an efficient and practical strategy, and their inhibitory activities against α-glucosidase and α-amylase were evaluated in vitro.
26207761	0	31	theme	Acid	50:53	arg1	Saponins					55:62	Oleanolic Acid Saponins	40:62	Oleanolic Acid Saponins as α-Glucosidase and α-Amylase Inhibitors	40:104	Synthesis and Evaluation of a Series of Oleanolic Acid Saponins as α-Glucosidase and α-Amylase Inhibitors.
26207761	3	32	theme	terminal	648:655	arg1	residue					677:683	a terminal α-l-rhamnopyranosyl residue	646:683	a terminal α-l-rhamnopyranosyl residue	646:683	Based on the preliminary structure-activity relationships, for 28-O-monoglucosides, the presence of a terminal α-l-rhamnopyranosyl residue enhanced the α-glucosidase and α-amylase inhibitory activities.
28917892	0	0	theme	xanthan	69:75	arg1	gum					77:79	xanthan gum	69:79	xanthan gum	69:79	A mechanism for the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures.
28917892	1	1	theme	gelling	186:192	arg1	properties					194:203	enhanced gelling properties	177:203	enhanced gelling properties	177:203	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	5	2	dep	weight	895:900	arg1	causes					902:907	causes	902:907	causes the opposite effect due to diffusion limitations	902:956	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	6	3	theme	gelling	1044:1050	arg1	agents					1052:1057	gelling agents	1044:1057	gelling agents	1044:1057	This work provides a set of fundamental guidelines to design novel thickeners and/or gelling agents based on proteins and polysaccharides, for food or pharmaceutical applications.
28917892	2	4	theme	co-localized	297:308	arg1	networks					310:317	co-localized networks	297:317	co-localized networks of GB and XG-rich domains	297:343	The mixed gels comprise co-localized networks of GB and XG-rich domains.
28917892	0	5	theme	aqueous	81:87	arg1	mixtures					89:96	aqueous mixtures	81:96	aqueous mixtures	81:96	A mechanism for the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures.
28917892	3	6	theme	GB	527:528	arg1	helices					537:543	GB triple helices	527:543	GB triple helices	527:543	Our results show that these domains are composed of intermolecular complexes and their aggregates stabilized by the neutralization effect of GB, and linked together by formation of GB triple helices.
28917892	4	7	theme	network	721:727	arg1	ratio=8-10					744:753	ratio=8-10	744:753	ratio=8-10	744:753	GB/XG mixtures display composition-dependent microstructural transitions: from discontinuous aggregates (GB/XG ratio≤1) to a continuous GB/XG network (ratio=2-6), followed by network fragmentation (ratio=8-10).
28917892	4	7	theme	network	721:727	arg1	fragmentation					729:741	network fragmentation	721:741	network fragmentation (ratio=8-10)	721:754	GB/XG mixtures display composition-dependent microstructural transitions: from discontinuous aggregates (GB/XG ratio≤1) to a continuous GB/XG network (ratio=2-6), followed by network fragmentation (ratio=8-10).
28917892	0	8	theme	gum	77:79	arg1	properties					41:50	the synergistic gelation properties	16:50	the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures	16:96	A mechanism for the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures.
28917892	4	9	theme	GB/XG	682:686	arg1	ratio=2-6					697:705	ratio=2-6	697:705	ratio=2-6	697:705	GB/XG mixtures display composition-dependent microstructural transitions: from discontinuous aggregates (GB/XG ratio≤1) to a continuous GB/XG network (ratio=2-6), followed by network fragmentation (ratio=8-10).
28917892	4	9	theme	GB/XG	682:686	arg1	network					688:694	a continuous GB/XG network	669:694	a continuous GB/XG network (ratio=2-6)	669:706	GB/XG mixtures display composition-dependent microstructural transitions: from discontinuous aggregates (GB/XG ratio≤1) to a continuous GB/XG network (ratio=2-6), followed by network fragmentation (ratio=8-10).
28917892	5	10	theme	network	799:805	arg1	formation					807:815	network formation	799:815	network formation	799:815	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	4	11	theme	microstructural	591:605	arg1	transitions					607:617	composition-dependent microstructural transitions	569:617	composition-dependent microstructural transitions:	569:618	GB/XG mixtures display composition-dependent microstructural transitions: from discontinuous aggregates (GB/XG ratio≤1) to a continuous GB/XG network (ratio=2-6), followed by network fragmentation (ratio=8-10).
28917892	3	12	theme	GB	487:488	arg1	effect					477:482	the neutralization effect	458:482	the neutralization effect of GB	458:488	Our results show that these domains are composed of intermolecular complexes and their aggregates stabilized by the neutralization effect of GB, and linked together by formation of GB triple helices.
28917892	5	13	theme	higher	832:837	arg1	G					856:856	G'	856:857	G'	856:857	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	5	13	theme	higher	832:837	arg1	modulus					847:853	higher elastic modulus	832:853	higher elastic modulus (G')	832:858	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	6	14	theme	novel	1020:1024	arg1	thickeners					1026:1035	novel thickeners	1020:1035	novel thickeners	1020:1035	This work provides a set of fundamental guidelines to design novel thickeners and/or gelling agents based on proteins and polysaccharides, for food or pharmaceutical applications.
28917892	2	15	theme	domains	337:343	arg1	networks					310:317	co-localized networks	297:317	co-localized networks of GB and XG-rich domains	297:343	The mixed gels comprise co-localized networks of GB and XG-rich domains.
28917892	6	16	theme	food	1102:1105	arg1	applications					1125:1136	food or pharmaceutical applications	1102:1136	food or pharmaceutical applications	1102:1136	This work provides a set of fundamental guidelines to design novel thickeners and/or gelling agents based on proteins and polysaccharides, for food or pharmaceutical applications.
28917892	2	17	theme	XG-rich	329:335	arg1	domains					337:343	XG-rich domains	329:343	XG-rich domains	329:343	The mixed gels comprise co-localized networks of GB and XG-rich domains.
28917892	5	18	theme	GB	772:773	arg1	index					781:785	the GB Bloom index	768:785	the GB Bloom index	768:785	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	5	19	theme	XG	882:883	arg1	weight					895:900	the XG molecular weight	878:900	the XG molecular weight causes the opposite effect due to diffusion limitations	878:956	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	1	20	theme	0.2-2	151:155	arg1	w/v					164:166	0.2-2%)/0.2% w/v	151:166	0.2-2%)/0.2% w/v	151:166	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	5	21	theme	Bloom	775:779	arg1	index					781:785	the GB Bloom index	768:785	the GB Bloom index	768:785	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	1	22	theme	pure	223:226	arg1	solutions					238:246	their pure component solutions	217:246	their pure component solutions	217:246	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	0	23	dep	B	63:63	arg1	mixtures					89:96	aqueous mixtures	81:96	aqueous mixtures	81:96	A mechanism for the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures.
28917892	1	24	theme	%	156:156	arg1	w/v					164:166	0.2-2%)/0.2% w/v	151:166	0.2-2%)/0.2% w/v	151:166	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	6	25	dep	guidelines	999:1008	arg1	design					1013:1018	design	1013:1018	to design novel thickeners and/or gelling agents based on proteins and polysaccharides	1010:1095	This work provides a set of fundamental guidelines to design novel thickeners and/or gelling agents based on proteins and polysaccharides, for food or pharmaceutical applications.
28917892	1	26	theme	component	228:236	arg1	solutions					238:246	their pure component solutions	217:246	their pure component solutions	217:246	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	5	27	theme	opposite	913:920	arg1	effect					922:927	the opposite effect	909:927	the opposite effect due to diffusion limitations	909:956	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	3	28	theme	neutralization	462:475	arg1	effect					477:482	the neutralization effect	458:482	the neutralization effect of GB	458:488	Our results show that these domains are composed of intermolecular complexes and their aggregates stabilized by the neutralization effect of GB, and linked together by formation of GB triple helices.
28917892	2	29	theme	GB	322:323	arg1	networks					310:317	co-localized networks	297:317	co-localized networks of GB and XG-rich domains	297:343	The mixed gels comprise co-localized networks of GB and XG-rich domains.
28917892	0	30	theme	gelation	32:39	arg1	properties					41:50	the synergistic gelation properties	16:50	the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures	16:96	A mechanism for the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures.
28917892	4	31	theme	continuous	671:680	arg1	ratio=2-6					697:705	ratio=2-6	697:705	ratio=2-6	697:705	GB/XG mixtures display composition-dependent microstructural transitions: from discontinuous aggregates (GB/XG ratio≤1) to a continuous GB/XG network (ratio=2-6), followed by network fragmentation (ratio=8-10).
28917892	4	31	theme	continuous	671:680	arg1	network					688:694	a continuous GB/XG network	669:694	a continuous GB/XG network (ratio=2-6)	669:706	GB/XG mixtures display composition-dependent microstructural transitions: from discontinuous aggregates (GB/XG ratio≤1) to a continuous GB/XG network (ratio=2-6), followed by network fragmentation (ratio=8-10).
28917892	3	32	theme	helices	537:543	arg1	formation					514:522	formation	514:522	formation of GB triple helices	514:543	Our results show that these domains are composed of intermolecular complexes and their aggregates stabilized by the neutralization effect of GB, and linked together by formation of GB triple helices.
28917892	5	33	theme	elastic	839:845	arg1	G					856:856	G'	856:857	G'	856:857	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	5	33	theme	elastic	839:845	arg1	modulus					847:853	higher elastic modulus	832:853	higher elastic modulus (G')	832:858	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	0	34	theme	synergistic	20:30	arg1	properties					41:50	the synergistic gelation properties	16:50	the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures	16:96	A mechanism for the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures.
28917892	1	35	theme	%	162:162	arg1	w/v					164:166	0.2-2%)/0.2% w/v	151:166	0.2-2%)/0.2% w/v	151:166	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	1	36	theme	similar	251:257	arg1	compositions					259:270	similar compositions	251:270	similar compositions	251:270	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	1	37	dep	GB/XG	143:147	arg1	w/v					164:166	0.2-2%)/0.2% w/v	151:166	0.2-2%)/0.2% w/v	151:166	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	1	38	theme	Gelatin	99:105	arg1	B					107:107	Gelatin B	99:107	Gelatin B	99:107	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	3	39	theme	triple	530:535	arg1	helices					537:543	GB triple helices	527:543	GB triple helices	527:543	Our results show that these domains are composed of intermolecular complexes and their aggregates stabilized by the neutralization effect of GB, and linked together by formation of GB triple helices.
28917892	1	40	theme	B	107:107	arg1	GB/XG					143:147	GB/XG	143:147	GB/XG	143:147	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	1	40	theme	B	107:107	arg1	mixtures					133:140	Gelatin B and xanthan gum aqueous mixtures	99:140	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v)	99:167	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	6	41	theme	pharmaceutical	1110:1123	arg1	applications					1125:1136	food or pharmaceutical applications	1102:1136	food or pharmaceutical applications	1102:1136	This work provides a set of fundamental guidelines to design novel thickeners and/or gelling agents based on proteins and polysaccharides, for food or pharmaceutical applications.
28917892	5	42	theme	due	929:931	arg1	effect					922:927	the opposite effect	909:927	the opposite effect due to diffusion limitations	909:956	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	2	43	theme	mixed	277:281	arg1	gels					283:286	The mixed gels	273:286	The mixed gels	273:286	The mixed gels comprise co-localized networks of GB and XG-rich domains.
28917892	5	44	theme	molecular	885:893	arg1	weight					895:900	the XG molecular weight	878:900	the XG molecular weight causes the opposite effect due to diffusion limitations	878:956	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	6	45	theme	guidelines	999:1008	arg1	set					980:982	a set	978:982	a set of fundamental guidelines to design novel thickeners and/or gelling agents based on proteins and polysaccharides	978:1095	This work provides a set of fundamental guidelines to design novel thickeners and/or gelling agents based on proteins and polysaccharides, for food or pharmaceutical applications.
28917892	6	45	theme	guidelines	999:1008	arg1	guidelines					999:1008	fundamental guidelines	987:1008	fundamental guidelines to design novel thickeners and/or gelling agents based on proteins and polysaccharides	987:1095	This work provides a set of fundamental guidelines to design novel thickeners and/or gelling agents based on proteins and polysaccharides, for food or pharmaceutical applications.
28917892	1	46	theme	xanthan	113:119	arg1	gum					121:123	xanthan gum	113:123	xanthan gum	113:123	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	4	47	theme	GB/XG	546:550	arg1	mixtures					552:559	GB/XG mixtures	546:559	GB/XG mixtures	546:559	GB/XG mixtures display composition-dependent microstructural transitions: from discontinuous aggregates (GB/XG ratio≤1) to a continuous GB/XG network (ratio=2-6), followed by network fragmentation (ratio=8-10).
28917892	0	48	theme	B	63:63	arg1	properties					41:50	the synergistic gelation properties	16:50	the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures	16:96	A mechanism for the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures.
28917892	3	49	theme	intermolecular	398:411	arg1	complexes					413:421	intermolecular complexes	398:421	intermolecular complexes	398:421	Our results show that these domains are composed of intermolecular complexes and their aggregates stabilized by the neutralization effect of GB, and linked together by formation of GB triple helices.
28917892	5	50	theme	diffusion	936:944	arg1	limitations					946:956	diffusion limitations	936:956	diffusion limitations	936:956	Increasing the GB Bloom index accelerates network formation and results in higher elastic modulus (G'), while increasing the XG molecular weight causes the opposite effect due to diffusion limitations.
28917892	6	51	theme	fundamental	987:997	arg1	guidelines					999:1008	fundamental guidelines	987:1008	fundamental guidelines to design novel thickeners and/or gelling agents based on proteins and polysaccharides	987:1095	This work provides a set of fundamental guidelines to design novel thickeners and/or gelling agents based on proteins and polysaccharides, for food or pharmaceutical applications.
28917892	1	52	theme	gum	121:123	arg1	GB/XG					143:147	GB/XG	143:147	GB/XG	143:147	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	1	52	theme	gum	121:123	arg1	mixtures					133:140	Gelatin B and xanthan gum aqueous mixtures	99:140	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v)	99:167	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	4	53	theme	discontinuous	625:637	arg1	aggregates					639:648	discontinuous aggregates	625:648	discontinuous aggregates (GB/XG ratio≤1)	625:664	GB/XG mixtures display composition-dependent microstructural transitions: from discontinuous aggregates (GB/XG ratio≤1) to a continuous GB/XG network (ratio=2-6), followed by network fragmentation (ratio=8-10).
28917892	4	53	theme	discontinuous	625:637	arg1	ratio≤1					657:663	GB/XG ratio≤1	651:663	GB/XG ratio≤1	651:663	GB/XG mixtures display composition-dependent microstructural transitions: from discontinuous aggregates (GB/XG ratio≤1) to a continuous GB/XG network (ratio=2-6), followed by network fragmentation (ratio=8-10).
28917892	4	54	theme	composition-dependent	569:589	arg1	transitions					607:617	composition-dependent microstructural transitions	569:617	composition-dependent microstructural transitions:	569:618	GB/XG mixtures display composition-dependent microstructural transitions: from discontinuous aggregates (GB/XG ratio≤1) to a continuous GB/XG network (ratio=2-6), followed by network fragmentation (ratio=8-10).
28917892	0	55	theme	gelatin	55:61	arg1	B					63:63	gelatin B	55:63	gelatin B	55:63	A mechanism for the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures.
28917892	1	56	theme	aqueous	125:131	arg1	GB/XG					143:147	GB/XG	143:147	GB/XG	143:147	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	1	56	theme	aqueous	125:131	arg1	mixtures					133:140	Gelatin B and xanthan gum aqueous mixtures	99:140	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v)	99:167	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
28917892	1	57	theme	enhanced	177:184	arg1	properties					194:203	enhanced gelling properties	177:203	enhanced gelling properties	177:203	Gelatin B and xanthan gum aqueous mixtures (GB/XG, (0.2-2%)/0.2% w/v) exhibit enhanced gelling properties compared to their pure component solutions at similar compositions.
26721230	9	0	theme	dietary	1689:1695	arg1	incorporation					1703:1715	dietary scFOS incorporation	1689:1715	dietary scFOS incorporation	1689:1715	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	1	1	theme	Sparus	392:397	arg1	bream					385:389	gilthead sea bream	372:389	gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks	372:543	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	1	theme	Sparus	392:397	arg1	aurata					399:404	Sparus aurata	392:404	Sparus aurata	392:404	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	2	theme	incorporation	228:240	arg1	effects					166:172	The effects	162:172	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology,	162:353	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	3	dep	%	522:522	arg1	0.5					519:521	0.5	519:521	0.5	519:521	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	3	dep	%	522:522	arg1	0.25					510:513	0.25	510:513	0.25	510:513	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	8	4	theme	Gut	1483:1485	arg1	morphology					1487:1496	Gut morphology	1483:1496	Gut morphology	1483:1496	Gut morphology was also unaffected by dietary prebiotic incorporation.
26721230	5	5	theme	scFOS	1159:1163	arg1	diets					1165:1169	0.1% and 0.5% scFOS diets	1145:1169	0.1% and 0.5% scFOS diets	1145:1169	In fish fed scFOS, lymphocytes were higher at 18 °C. Total Ig were also higher at 18 °C but only in fish fed 0.1% and 0.5% scFOS diets.
26721230	9	6	theme	digestive	1591:1599	arg1	enzymes					1601:1607	digestive enzymes	1591:1607	digestive enzymes activities	1591:1618	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	4	7	theme	nitric	725:730	arg1	NO					739:740	NO	739:740	NO	739:740	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	4	7	theme	nitric	725:730	arg1	oxide					732:736	nitric oxide	725:736	increased nitric oxide (NO) production	715:752	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	4	8	theme	white	943:947	arg1	cells					955:959	total white blood cells	937:959	total white blood cells	937:959	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	6	9	theme	gel	1286:1288	arg1	electrophoresis					1290:1304	gradient gel electrophoresis	1277:1304	gradient gel electrophoresis	1277:1304	No differences in gut bacterial profiles were detected by PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) between dietary treatments.
26721230	0	10	theme	sea	108:110	arg1	bream					112:116	gilthead sea bream	99:116	gilthead sea bream (Sparus aurata)	99:132	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	0	10	theme	sea	108:110	arg1	aurata					126:131	Sparus aurata	119:131	Sparus aurata	119:131	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	8	11	theme	dietary	1521:1527	arg1	incorporation					1539:1551	dietary prebiotic incorporation	1521:1551	dietary prebiotic incorporation	1521:1551	Gut morphology was also unaffected by dietary prebiotic incorporation.
26721230	9	12	theme	scFOS	1697:1701	arg1	incorporation					1703:1715	dietary scFOS incorporation	1689:1715	dietary scFOS incorporation	1689:1715	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	3	13	theme	%	662:662	arg1	diet					670:673	the 0.1% scFOS diet	655:673	the 0.1% scFOS diet	655:673	Lymphocytes decreased in fish fed the 0.1% scFOS diet.
26721230	1	14	theme	short	185:189	arg1	scFOS					221:225	scFOS	221:225	scFOS	221:225	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	14	theme	short	185:189	arg1	fructooligosaccharides					197:218	dietary short chain fructooligosaccharides	177:218	dietary short chain fructooligosaccharides (scFOS) incorporation	177:240	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	7	15	theme	Digestive	1384:1392	arg1	enzymes					1394:1400	Digestive enzymes	1384:1400	25 °C. Digestive enzymes activities	1377:1411	However, group's similarity was higher at 25 °C. Digestive enzymes activities were higher at 25 °C but were unaffected by prebiotics incorporation.
26721230	2	16	theme	sea	596:598	arg1	bream					600:604	gilthead sea bream	587:604	gilthead sea bream immune status	587:618	Overall, scFOS had only minor effects on gilthead sea bream immune status.
26721230	10	17	theme	tested	1783:1788	arg1	levels					1790:1795	the tested levels	1779:1795	the tested levels	1779:1795	In conclusion, at the tested levels scFOS does not seem worthy of including it in gilthead sea bream juveniles diets.
26721230	1	18	theme	gilthead	372:379	arg1	bream					385:389	gilthead sea bream	372:389	gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks	372:543	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	18	theme	gilthead	372:379	arg1	aurata					399:404	Sparus aurata	392:404	Sparus aurata	392:404	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	19	from	effects	166:172	arg1	morphology					343:352	gut morphology	339:352	gut morphology	339:352	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	19	from	effects	166:172	arg1	status					269:274	fish immune status	257:274	fish immune status	257:274	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	19	from	effects	166:172	arg1	activities					323:332	digestive enzymes activities	305:332	digestive enzymes activities	305:332	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	19	from	effects	166:172	arg1	composition					292:302	gut microbiota composition	277:302	gut microbiota composition	277:302	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	19	from	effects	166:172	arg1	hematology					245:254	hematology	245:254	hematology	245:254	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	4	20	theme	total	761:765	arg1	Ig					784:785	Ig	784:785	Ig	784:785	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	4	20	theme	total	761:765	arg1	immunoglobulins					767:781	total immunoglobulins	761:781	total immunoglobulins (Ig)	761:786	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	1	21	theme	bream	385:389	arg1	juveniles					407:415	gilthead sea bream (Sparus aurata) juveniles	372:415	gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks	372:543	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	5	22	theme	Total	1089:1093	arg1	Ig					1095:1096	18 °C. Total Ig	1082:1096	18 °C. Total Ig	1082:1096	In fish fed scFOS, lymphocytes were higher at 18 °C. Total Ig were also higher at 18 °C but only in fish fed 0.1% and 0.5% scFOS diets.
26721230	4	23	theme	blood	853:857	arg1	cells					859:863	25 °C. Red blood cells	842:863	25 °C. Red blood cells	842:863	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	1	24	dep	18 °C	427:431	arg1	fed					482:484	fed	482:484	were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks	477:543	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	2	25	theme	immune	606:611	arg1	status					613:618	gilthead sea bream immune status	587:618	gilthead sea bream immune status	587:618	Overall, scFOS had only minor effects on gilthead sea bream immune status.
26721230	1	26	theme	enzymes	315:321	arg1	activities					323:332	digestive enzymes activities	305:332	digestive enzymes activities	305:332	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	26	theme	enzymes	315:321	arg1	hematology					245:254	hematology	245:254	hematology	245:254	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	0	27	from	Effect	0:5	arg1	status					70:75	immunological status	56:75	immunological status	56:75	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	0	27	from	Effect	0:5	arg1	microbiota					85:94	gut microbiota	81:94	gut microbiota	81:94	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	0	28	theme	gut	81:83	arg1	microbiota					85:94	gut microbiota	81:94	gut microbiota	81:94	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	7	29	theme	prebiotics	1457:1466	arg1	incorporation					1468:1480	prebiotics incorporation	1457:1480	prebiotics incorporation	1457:1480	However, group's similarity was higher at 25 °C. Digestive enzymes activities were higher at 25 °C but were unaffected by prebiotics incorporation.
26721230	9	30	theme	microbiota	1567:1576	arg1	composition					1578:1588	gut microbiota composition	1563:1588	gut microbiota composition	1563:1588	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	6	31	theme	dietary	1315:1321	arg1	treatments					1323:1332	dietary treatments	1315:1332	dietary treatments	1315:1332	No differences in gut bacterial profiles were detected by PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) between dietary treatments.
26721230	1	32	theme	gut	339:341	arg1	hematology					245:254	hematology	245:254	hematology	245:254	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	32	theme	gut	339:341	arg1	morphology					343:352	gut morphology	339:352	gut morphology	339:352	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	4	33	theme	Red	849:851	arg1	cells					859:863	25 °C. Red blood cells	842:863	25 °C. Red blood cells	842:863	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	2	34	theme	minor	570:574	arg1	effects					576:582	only minor effects	565:582	only minor effects	565:582	Overall, scFOS had only minor effects on gilthead sea bream immune status.
26721230	6	35	theme	gut	1190:1192	arg1	profiles					1204:1211	gut bacterial profiles	1190:1211	gut bacterial profiles	1190:1211	No differences in gut bacterial profiles were detected by PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) between dietary treatments.
26721230	9	36	theme	enzymes	1601:1607	arg1	activities					1609:1618	digestive enzymes activities	1591:1618	digestive enzymes activities	1591:1618	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	0	37	theme	short	10:14	arg1	scFOS					46:50	scFOS	46:50	scFOS	46:50	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	0	37	theme	short	10:14	arg1	fructooligosaccharides					22:43	short chain fructooligosaccharides	10:43	short chain fructooligosaccharides (scFOS)	10:51	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	1	38	theme	immune	262:267	arg1	status					269:274	fish immune status	257:274	fish immune status	257:274	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	38	theme	immune	262:267	arg1	hematology					245:254	hematology	245:254	hematology	245:254	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	4	39	theme	0.5	689:691	arg1	%					692:692	%	692:692	%	692:692	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	0	40	theme	fructooligosaccharides	22:43	arg1	Effect					0:5	Effect	0:5	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata)	0:132	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	4	41	theme	scFOS	694:698	arg1	diet					700:703	the 0.5% scFOS diet	685:703	the 0.5% scFOS diet	685:703	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	1	42	theme	microbiota	281:290	arg1	hematology					245:254	hematology	245:254	hematology	245:254	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	42	theme	microbiota	281:290	arg1	composition					292:302	gut microbiota composition	277:302	gut microbiota composition	277:302	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	5	43	theme	%	1157:1157	arg1	diets					1165:1169	0.1% and 0.5% scFOS diets	1145:1169	0.1% and 0.5% scFOS diets	1145:1169	In fish fed scFOS, lymphocytes were higher at 18 °C. Total Ig were also higher at 18 °C but only in fish fed 0.1% and 0.5% scFOS diets.
26721230	6	44	theme	polymerase	1240:1249	arg1	reaction					1257:1264	polymerase chain reaction	1240:1264	polymerase chain reaction denaturing gradient gel electrophoresis	1240:1304	No differences in gut bacterial profiles were detected by PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) between dietary treatments.
26721230	1	45	theme	chain	191:195	arg1	scFOS					221:225	scFOS	221:225	scFOS	221:225	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	45	theme	chain	191:195	arg1	fructooligosaccharides					197:218	dietary short chain fructooligosaccharides	177:218	dietary short chain fructooligosaccharides (scFOS) incorporation	177:240	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	9	46	theme	rearing	1661:1667	arg1	temperature					1669:1679	rearing temperature	1661:1679	rearing temperature	1661:1679	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	9	47	theme	minor	1726:1730	arg1	effects					1732:1738	only minor effects	1721:1738	only minor effects	1721:1738	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	5	48	theme	fish	1039:1042	arg1	scFOS					1048:1052	fish fed scFOS	1039:1052	fish fed scFOS	1039:1052	In fish fed scFOS, lymphocytes were higher at 18 °C. Total Ig were also higher at 18 °C but only in fish fed 0.1% and 0.5% scFOS diets.
26721230	1	49	with	fish	462:465	arg1	32 g					472:475	32 g	472:475	32 g	472:475	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	5	50	theme	0.1	1145:1147	arg1	%					1148:1148	%	1148:1148	%	1148:1148	In fish fed scFOS, lymphocytes were higher at 18 °C. Total Ig were also higher at 18 °C but only in fish fed 0.1% and 0.5% scFOS diets.
26721230	4	51	theme	oxide	732:736	arg1	production					743:752	increased nitric oxide (NO) production	715:752	increased nitric oxide (NO) production	715:752	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	4	52	theme	bactericidal	878:889	arg1	activity					891:898	bactericidal activity	878:898	bactericidal activity	878:898	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	9	53	contain	had	1717:1719	arg2	effects					1732:1738	only minor effects	1721:1738	only minor effects	1721:1738	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	9	53	contain	had	1717:1719	arg1	incorporation					1703:1715	dietary scFOS incorporation	1689:1715	dietary scFOS incorporation	1689:1715	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	4	54	theme	circulating	962:972	arg1	thrombocytes					974:985	circulating thrombocytes	962:985	circulating thrombocytes	962:985	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	4	55	theme	total	937:941	arg1	cells					955:959	total white blood cells	937:959	total white blood cells	937:959	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	4	56	dep	fed	681:683	arg1	presented					705:713	presented	705:713	presented	705:713	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	4	57	theme	blood	949:953	arg1	cells					955:959	total white blood cells	937:959	total white blood cells	937:959	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	4	58	theme	increased	715:723	arg1	production					743:752	increased nitric oxide (NO) production	715:752	increased nitric oxide (NO) production	715:752	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	1	59	dep	scFOS	524:528	arg1	%					522:522	%	522:522	%	522:522	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	0	60	theme	bream	112:116	arg1	status					70:75	immunological status	56:75	immunological status	56:75	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	0	60	theme	bream	112:116	arg1	microbiota					85:94	gut microbiota	81:94	gut microbiota	81:94	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	6	61	theme	gradient	1277:1284	arg1	electrophoresis					1290:1304	gradient gel electrophoresis	1277:1304	gradient gel electrophoresis	1277:1304	No differences in gut bacterial profiles were detected by PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) between dietary treatments.
26721230	7	62	theme	enzymes	1394:1400	arg1	activities					1402:1411	25 °C. Digestive enzymes activities	1377:1411	25 °C. Digestive enzymes activities	1377:1411	However, group's similarity was higher at 25 °C. Digestive enzymes activities were higher at 25 °C but were unaffected by prebiotics incorporation.
26721230	2	63	contain	had	561:563	arg2	effects					576:582	only minor effects	565:582	only minor effects	565:582	Overall, scFOS had only minor effects on gilthead sea bream immune status.
26721230	2	63	contain	had	561:563	arg1	scFOS					555:559	scFOS	555:559	scFOS	555:559	Overall, scFOS had only minor effects on gilthead sea bream immune status.
26721230	0	64	theme	Sparus	119:124	arg1	bream					112:116	gilthead sea bream	99:116	gilthead sea bream (Sparus aurata)	99:132	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	0	64	theme	Sparus	119:124	arg1	aurata					126:131	Sparus aurata	119:131	Sparus aurata	119:131	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	6	65	from	differences	1175:1185	arg1	profiles					1204:1211	gut bacterial profiles	1190:1211	gut bacterial profiles	1190:1211	No differences in gut bacterial profiles were detected by PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) between dietary treatments.
26721230	1	66	theme	dietary	177:183	arg1	scFOS					221:225	scFOS	221:225	scFOS	221:225	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	66	theme	dietary	177:183	arg1	fructooligosaccharides					197:218	dietary short chain fructooligosaccharides	177:218	dietary short chain fructooligosaccharides (scFOS) incorporation	177:240	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	7	67	theme	25 °C.	1377:1382	arg1	activities					1402:1411	25 °C. Digestive enzymes activities	1377:1411	25 °C. Digestive enzymes activities	1377:1411	However, group's similarity was higher at 25 °C. Digestive enzymes activities were higher at 25 °C but were unaffected by prebiotics incorporation.
26721230	2	68	theme	bream	600:604	arg1	status					613:618	gilthead sea bream immune status	587:618	gilthead sea bream immune status	587:618	Overall, scFOS had only minor effects on gilthead sea bream immune status.
26721230	3	69	theme	scFOS	664:668	arg1	diet					670:673	the 0.1% scFOS diet	655:673	the 0.1% scFOS diet	655:673	Lymphocytes decreased in fish fed the 0.1% scFOS diet.
26721230	8	70	theme	prebiotic	1529:1537	arg1	incorporation					1539:1551	dietary prebiotic incorporation	1521:1551	dietary prebiotic incorporation	1521:1551	Gut morphology was also unaffected by dietary prebiotic incorporation.
26721230	2	71	theme	gilthead	587:594	arg1	bream					600:604	gilthead sea bream	587:604	gilthead sea bream immune status	587:618	Overall, scFOS had only minor effects on gilthead sea bream immune status.
26721230	3	72	theme	0.1	659:661	arg1	%					662:662	%	662:662	%	662:662	Lymphocytes decreased in fish fed the 0.1% scFOS diet.
26721230	1	73	theme	sea	381:383	arg1	bream					385:389	gilthead sea bream	372:389	gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks	372:543	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	73	theme	sea	381:383	arg1	aurata					399:404	Sparus aurata	392:404	Sparus aurata	392:404	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	5	74	theme	18 °C.	1082:1087	arg1	Ig					1095:1096	18 °C. Total Ig	1082:1096	18 °C. Total Ig	1082:1096	In fish fed scFOS, lymphocytes were higher at 18 °C. Total Ig were also higher at 18 °C but only in fish fed 0.1% and 0.5% scFOS diets.
26721230	0	75	theme	immunological	56:68	arg1	status					70:75	immunological status	56:75	immunological status	56:75	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	1	76	theme	digestive	305:313	arg1	enzymes					315:321	digestive enzymes	305:321	digestive enzymes activities	305:332	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	9	77	theme	gut	1563:1565	arg1	composition					1578:1588	gut microbiota composition	1563:1588	gut microbiota composition	1563:1588	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	0	78	theme	gilthead	99:106	arg1	bream					112:116	gilthead sea bream	99:116	gilthead sea bream (Sparus aurata)	99:132	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	0	78	theme	gilthead	99:106	arg1	aurata					126:131	Sparus aurata	119:131	Sparus aurata	119:131	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	10	79	theme	sea	1852:1854	arg1	bream					1856:1860	gilthead sea bream	1843:1860	gilthead sea bream	1843:1860	In conclusion, at the tested levels scFOS does not seem worthy of including it in gilthead sea bream juveniles diets.
26721230	9	80	theme	composition	1578:1588	arg1	parameters					1633:1642	gut microbiota composition, digestive enzymes activities and immunity parameters	1563:1642	gut microbiota composition, digestive enzymes activities and immunity parameters	1563:1642	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	6	81	theme	bacterial	1194:1202	arg1	profiles					1204:1211	gut bacterial profiles	1190:1211	gut bacterial profiles	1190:1211	No differences in gut bacterial profiles were detected by PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) between dietary treatments.
26721230	4	82	theme	25 °C.	842:847	arg1	cells					859:863	25 °C. Red blood cells	842:863	25 °C. Red blood cells	842:863	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	0	83	theme	chain	16:20	arg1	scFOS					46:50	scFOS	46:50	scFOS	46:50	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	0	83	theme	chain	16:20	arg1	fructooligosaccharides					22:43	short chain fructooligosaccharides	10:43	short chain fructooligosaccharides (scFOS)	10:51	Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.
26721230	1	84	theme	fish	257:260	arg1	status					269:274	fish immune status	257:274	fish immune status	257:274	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	84	theme	fish	257:260	arg1	hematology					245:254	hematology	245:254	hematology	245:254	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	9	85	theme	activities	1609:1618	arg1	parameters					1633:1642	gut microbiota composition, digestive enzymes activities and immunity parameters	1563:1642	gut microbiota composition, digestive enzymes activities and immunity parameters	1563:1642	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	9	86	theme	immunity	1624:1631	arg1	parameters					1633:1642	gut microbiota composition, digestive enzymes activities and immunity parameters	1563:1642	gut microbiota composition, digestive enzymes activities and immunity parameters	1563:1642	Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters.
26721230	5	87	theme	%	1148:1148	arg1	diets					1165:1169	0.1% and 0.5% scFOS diets	1145:1169	0.1% and 0.5% scFOS diets	1145:1169	In fish fed scFOS, lymphocytes were higher at 18 °C. Total Ig were also higher at 18 °C but only in fish fed 0.1% and 0.5% scFOS diets.
26721230	1	88	theme	gut	277:279	arg1	hematology					245:254	hematology	245:254	hematology	245:254	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	1	88	theme	gut	277:279	arg1	composition					292:302	gut microbiota composition	277:302	gut microbiota composition	277:302	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	4	89	theme	%	692:692	arg1	diet					700:703	the 0.5% scFOS diet	685:703	the 0.5% scFOS diet	685:703	Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C.
26721230	5	90	theme	0.5	1154:1156	arg1	%					1157:1157	%	1157:1157	%	1157:1157	In fish fed scFOS, lymphocytes were higher at 18 °C. Total Ig were also higher at 18 °C but only in fish fed 0.1% and 0.5% scFOS diets.
26721230	6	91	theme	chain	1251:1255	arg1	reaction					1257:1264	polymerase chain reaction	1240:1264	polymerase chain reaction denaturing gradient gel electrophoresis	1240:1304	No differences in gut bacterial profiles were detected by PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) between dietary treatments.
26721230	1	92	theme	fructooligosaccharides	197:218	arg1	incorporation					228:240	dietary short chain fructooligosaccharides (scFOS) incorporation	177:240	dietary short chain fructooligosaccharides (scFOS) incorporation	177:240	The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks.
26721230	6	93	dep	PCR-DGGE	1230:1237	arg1	reaction					1257:1264	polymerase chain reaction	1240:1264	polymerase chain reaction denaturing gradient gel electrophoresis	1240:1304	No differences in gut bacterial profiles were detected by PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) between dietary treatments.
26721230	10	94	theme	gilthead	1843:1850	arg1	bream					1856:1860	gilthead sea bream	1843:1860	gilthead sea bream	1843:1860	In conclusion, at the tested levels scFOS does not seem worthy of including it in gilthead sea bream juveniles diets.
26721230	5	95	theme	fed	1044:1046	arg1	scFOS					1048:1052	fish fed scFOS	1039:1052	fish fed scFOS	1039:1052	In fish fed scFOS, lymphocytes were higher at 18 °C. Total Ig were also higher at 18 °C but only in fish fed 0.1% and 0.5% scFOS diets.
26256284	5	0	theme	cofactors	677:685	arg1	structure					659:667	the main structure	650:667	the main structure of some cofactors	650:685	In the present paper, the formation of a set of pterins from cyanide polymerizations is demonstrated, showing that the main structure of some cofactors can be prebiotically formed.
26256284	6	1	theme	NH4CN	838:842	arg1	polymers					844:851	the insoluble NH4CN polymers	824:851	the insoluble NH4CN polymers synthesized	824:863	Indeed, it was observed that aqueous aerosols additionally increase the relative composition for pterins in the insoluble NH4CN polymers synthesized.
26256284	7	2	from	pterins	894:900	arg1	polymers					911:918	NH4CN polymers	905:918	NH4CN polymers	905:918	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	7	3	theme	important	967:975	arg1	biomonomers					977:987	other important biomonomers	961:987	other important biomonomers	961:987	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	4	4	theme	cofactor	509:516	arg1	blocks					527:532	cofactor building blocks	509:532	cofactor building blocks	509:532	In contrast, Eschenmoser and Loewenthal suggested a prebiotic hydrocyanic origin of cofactor building blocks.
26256284	6	5	theme	insoluble	828:836	arg1	polymers					844:851	the insoluble NH4CN polymers	824:851	the insoluble NH4CN polymers synthesized	824:863	Indeed, it was observed that aqueous aerosols additionally increase the relative composition for pterins in the insoluble NH4CN polymers synthesized.
26256284	7	6	theme	pterins	894:900	arg1	identification					876:889	The novel identification	866:889	The novel identification of pterins in NH4CN polymers	866:918	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	2	7	dep	likely	237:242	arg1	likely					237:242	likely	237:242	likely	237:242	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	2	7	dep	likely	237:242	arg1	issue					230:234	a poorly explored issue	212:234	a poorly explored issue	212:234	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	2	7	dep	likely	237:242	arg1	synthesis					173:181	The prebiotic synthesis	159:181	The prebiotic synthesis of cofactors or coenzymes	159:207	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	5	8	theme	set	576:578	arg1	formation					561:569	the formation	557:569	the formation of a set of pterins from cyanide polymerizations	557:618	In the present paper, the formation of a set of pterins from cyanide polymerizations is demonstrated, showing that the main structure of some cofactors can be prebiotically formed.
26256284	4	9	theme	blocks	527:532	arg1	origin					499:504	a prebiotic hydrocyanic origin	475:504	a prebiotic hydrocyanic origin of cofactor building blocks	475:532	In contrast, Eschenmoser and Loewenthal suggested a prebiotic hydrocyanic origin of cofactor building blocks.
26256284	6	10	from	composition	797:807	arg1	polymers					844:851	the insoluble NH4CN polymers	824:851	the insoluble NH4CN polymers synthesized	824:863	Indeed, it was observed that aqueous aerosols additionally increase the relative composition for pterins in the insoluble NH4CN polymers synthesized.
26256284	7	11	theme	early	1051:1055	arg1	state					1057:1061	the early state	1047:1061	the early state of prebiotic chemistry	1047:1084	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	1	12	theme	life	49:52	arg1	hypothesis					68:77	a hypothesis	66:77	a hypothesis of evolutionary science that can be, at least partially, tested experimentally	66:156	The genesis of life on Earth is a hypothesis of evolutionary science that can be, at least partially, tested experimentally.
26256284	1	12	theme	life	49:52	arg1	genesis					38:44	The genesis	34:44	The genesis of life on Earth	34:61	The genesis of life on Earth is a hypothesis of evolutionary science that can be, at least partially, tested experimentally.
26256284	4	13	theme	building	518:525	arg1	blocks					527:532	cofactor building blocks	509:532	cofactor building blocks	509:532	In contrast, Eschenmoser and Loewenthal suggested a prebiotic hydrocyanic origin of cofactor building blocks.
26256284	5	14	theme	main	654:657	arg1	structure					659:667	the main structure	650:667	the main structure of some cofactors	650:685	In the present paper, the formation of a set of pterins from cyanide polymerizations is demonstrated, showing that the main structure of some cofactors can be prebiotically formed.
26256284	5	15	theme	present	542:548	arg1	paper					550:554	the present paper	538:554	the present paper	538:554	In the present paper, the formation of a set of pterins from cyanide polymerizations is demonstrated, showing that the main structure of some cofactors can be prebiotically formed.
26256284	1	16	from	genesis	38:44	arg1	Earth					57:61	Earth	57:61	Earth	57:61	The genesis of life on Earth is a hypothesis of evolutionary science that can be, at least partially, tested experimentally.
26256284	3	17	theme	molecular	379:387	arg1	fossils					389:395	molecular fossils	379:395	"molecular fossils" of an early phase of life	378:422	In this sense, it has been proposed that the cofactors are "molecular fossils" of an early phase of life.
26256284	0	18	theme	Prebiotic	2:10	arg1	Synthesis					12:20	A Prebiotic Synthesis	0:20	A Prebiotic Synthesis of Pterins	0:31	A Prebiotic Synthesis of Pterins.
26256284	4	19	theme	hydrocyanic	487:497	arg1	origin					499:504	a prebiotic hydrocyanic origin	475:504	a prebiotic hydrocyanic origin of cofactor building blocks	475:532	In contrast, Eschenmoser and Loewenthal suggested a prebiotic hydrocyanic origin of cofactor building blocks.
26256284	7	20	theme	novel	870:874	arg1	identification					876:889	The novel identification	866:889	The novel identification of pterins in NH4CN polymers	866:918	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	2	21	theme	plausible	274:282	arg1	conditions					294:303	plausible prebiotic conditions	274:303	plausible prebiotic conditions	274:303	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	4	22	theme	prebiotic	477:485	arg1	origin					499:504	a prebiotic hydrocyanic origin	475:504	a prebiotic hydrocyanic origin of cofactor building blocks	475:532	In contrast, Eschenmoser and Loewenthal suggested a prebiotic hydrocyanic origin of cofactor building blocks.
26256284	7	23	from	essential	1034:1042	arg1	state					1057:1061	the early state	1047:1061	the early state of prebiotic chemistry	1047:1084	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	7	24	theme	cyanide	1005:1011	arg1	polymerizations					1013:1027	cyanide polymerizations	1005:1027	cyanide polymerizations	1005:1027	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	6	25	theme	relative	788:795	arg1	composition					797:807	the relative composition	784:807	the relative composition for pterins in the insoluble NH4CN polymers synthesized	784:863	Indeed, it was observed that aqueous aerosols additionally increase the relative composition for pterins in the insoluble NH4CN polymers synthesized.
26256284	1	26	theme	science	95:101	arg1	hypothesis					68:77	a hypothesis	66:77	a hypothesis of evolutionary science that can be, at least partially, tested experimentally	66:156	The genesis of life on Earth is a hypothesis of evolutionary science that can be, at least partially, tested experimentally.
26256284	1	26	theme	science	95:101	arg1	genesis					38:44	The genesis	34:44	The genesis of life on Earth	34:61	The genesis of life on Earth is a hypothesis of evolutionary science that can be, at least partially, tested experimentally.
26256284	2	27	theme	coenzymes	199:207	arg1	likely					237:242	likely	237:242	likely	237:242	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	2	27	theme	coenzymes	199:207	arg1	issue					230:234	a poorly explored issue	212:234	a poorly explored issue	212:234	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	2	27	theme	coenzymes	199:207	arg1	synthesis					173:181	The prebiotic synthesis	159:181	The prebiotic synthesis of cofactors or coenzymes	159:207	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	7	28	dep	together	921:928	arg1	with					930:933	with	930:933	with	930:933	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	3	29	theme	fossils	389:395	arg1	"					396:396	"molecular fossils"	378:396	"molecular fossils" of an early phase of life	378:422	In this sense, it has been proposed that the cofactors are "molecular fossils" of an early phase of life.
26256284	3	29	theme	fossils	389:395	arg1	cofactors					364:372	the cofactors	360:372	the cofactors	360:372	In this sense, it has been proposed that the cofactors are "molecular fossils" of an early phase of life.
26256284	0	30	theme	Pterins	25:31	arg1	Synthesis					12:20	A Prebiotic Synthesis	0:20	A Prebiotic Synthesis of Pterins	0:31	A Prebiotic Synthesis of Pterins.
26256284	5	31	from	set	576:578	arg1	polymerizations					604:618	cyanide polymerizations	596:618	cyanide polymerizations	596:618	In the present paper, the formation of a set of pterins from cyanide polymerizations is demonstrated, showing that the main structure of some cofactors can be prebiotically formed.
26256284	7	32	theme	previous	939:946	arg1	detection					948:956	the previous detection	935:956	the previous detection of other important biomonomers	935:987	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	7	33	theme	prebiotic	1066:1074	arg1	chemistry					1076:1084	prebiotic chemistry	1066:1084	prebiotic chemistry	1066:1084	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	5	34	theme	pterins	583:589	arg1	pterins					583:589	pterins	583:589	pterins from cyanide polymerizations	583:618	In the present paper, the formation of a set of pterins from cyanide polymerizations is demonstrated, showing that the main structure of some cofactors can be prebiotically formed.
26256284	5	34	theme	pterins	583:589	arg1	set					576:578	a set	574:578	a set of pterins from cyanide polymerizations	574:618	In the present paper, the formation of a set of pterins from cyanide polymerizations is demonstrated, showing that the main structure of some cofactors can be prebiotically formed.
26256284	2	35	theme	cofactors	186:194	arg1	likely					237:242	likely	237:242	likely	237:242	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	2	35	theme	cofactors	186:194	arg1	issue					230:234	a poorly explored issue	212:234	a poorly explored issue	212:234	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	2	35	theme	cofactors	186:194	arg1	synthesis					173:181	The prebiotic synthesis	159:181	The prebiotic synthesis of cofactors or coenzymes	159:207	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	7	36	theme	chemistry	1076:1084	arg1	state					1057:1061	the early state	1047:1061	the early state of prebiotic chemistry	1047:1084	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	7	37	from	state	1057:1061	arg1	essential					1034:1042	essential	1034:1042	essential	1034:1042	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	7	38	theme	biomonomers	977:987	arg1	detection					948:956	the previous detection	935:956	the previous detection of other important biomonomers	935:987	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	6	39	theme	aqueous	745:751	arg1	aerosols					753:760	aqueous aerosols	745:760	aqueous aerosols	745:760	Indeed, it was observed that aqueous aerosols additionally increase the relative composition for pterins in the insoluble NH4CN polymers synthesized.
26256284	3	40	theme	life	419:422	arg1	phase					410:414	an early phase	401:414	an early phase of life	401:422	In this sense, it has been proposed that the cofactors are "molecular fossils" of an early phase of life.
26256284	5	41	theme	cyanide	596:602	arg1	polymerizations					604:618	cyanide polymerizations	596:618	cyanide polymerizations	596:618	In the present paper, the formation of a set of pterins from cyanide polymerizations is demonstrated, showing that the main structure of some cofactors can be prebiotically formed.
26256284	2	42	theme	prebiotic	163:171	arg1	likely					237:242	likely	237:242	likely	237:242	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	2	42	theme	prebiotic	163:171	arg1	issue					230:234	a poorly explored issue	212:234	a poorly explored issue	212:234	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	2	42	theme	prebiotic	163:171	arg1	synthesis					173:181	The prebiotic synthesis	159:181	The prebiotic synthesis of cofactors or coenzymes	159:207	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	2	43	theme	explored	221:228	arg1	likely					237:242	likely	237:242	likely	237:242	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	2	43	theme	explored	221:228	arg1	issue					230:234	a poorly explored issue	212:234	a poorly explored issue	212:234	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	2	43	theme	explored	221:228	arg1	synthesis					173:181	The prebiotic synthesis	159:181	The prebiotic synthesis of cofactors or coenzymes	159:207	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	3	44	theme	early	404:408	arg1	phase					410:414	an early phase	401:414	an early phase of life	401:422	In this sense, it has been proposed that the cofactors are "molecular fossils" of an early phase of life.
26256284	7	45	theme	other	961:965	arg1	biomonomers					977:987	other important biomonomers	961:987	other important biomonomers	961:987	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	2	46	theme	prebiotic	284:292	arg1	conditions					294:303	plausible prebiotic conditions	274:303	plausible prebiotic conditions	274:303	The prebiotic synthesis of cofactors or coenzymes is a poorly explored issue, likely because their formation under plausible prebiotic conditions is not clear.
26256284	5	47	from	polymerizations	604:618	arg1	pterins					583:589	pterins	583:589	pterins from cyanide polymerizations	583:618	In the present paper, the formation of a set of pterins from cyanide polymerizations is demonstrated, showing that the main structure of some cofactors can be prebiotically formed.
26256284	5	47	from	polymerizations	604:618	arg1	set					576:578	a set	574:578	a set of pterins from cyanide polymerizations	574:618	In the present paper, the formation of a set of pterins from cyanide polymerizations is demonstrated, showing that the main structure of some cofactors can be prebiotically formed.
26256284	7	48	from	identification	876:889	arg1	polymers					911:918	NH4CN polymers	905:918	NH4CN polymers	905:918	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	3	49	theme	phase	410:414	arg1	"					396:396	"molecular fossils"	378:396	"molecular fossils" of an early phase of life	378:422	In this sense, it has been proposed that the cofactors are "molecular fossils" of an early phase of life.
26256284	3	49	theme	phase	410:414	arg1	cofactors					364:372	the cofactors	360:372	the cofactors	360:372	In this sense, it has been proposed that the cofactors are "molecular fossils" of an early phase of life.
26256284	7	50	from	polymers	911:918	arg1	identification					876:889	The novel identification	866:889	The novel identification of pterins in NH4CN polymers	866:918	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
26256284	7	51	theme	NH4CN	905:909	arg1	polymers					911:918	NH4CN polymers	905:918	NH4CN polymers	905:918	The novel identification of pterins in NH4CN polymers, together with the previous detection of other important biomonomers, indicates that cyanide polymerizations were essential in the early state of prebiotic chemistry.
29269259	0	0	theme	biomedical	90:99	arg1	applications					101:112	biomedical applications	90:112	biomedical applications	90:112	Thermosensitive chitosan/phosphate hydrogel-composites fortified with Ag versus Ag@Pd for biomedical applications.
29269259	6	1	theme	Bactericidal	733:744	arg1	activities					746:755	Bactericidal activities	733:755	Bactericidal activities	733:755	Bactericidal activities were tested against gram- positive Staphylococcus aureus and gram-negative Pseudomonas aeruginosa.
29269259	3	2	theme	METHODS	391:397	arg1	gels					434:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels	377:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels	377:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels were successfully achieved.
29269259	8	3	dep	layers	1046:1051	arg1	surfaces					1071:1078	the scaffold surfaces	1058:1078	the scaffold surfaces	1058:1078	KEY FINDINGS The newly developed phosphatic layers over the scaffold surfaces post-immersion assessed their osteogenic ability.
29269259	9	4	theme	metals	1215:1220	arg1	incorporation					1222:1234	the noble metals incorporation	1205:1234	the noble metals incorporation	1205:1234	Further, their promising and differentiated bactericidal activities due to the noble metals incorporation were proved.
29269259	7	5	theme	DMEM	884:887	arg1	immersion					889:897	DMEM immersion	884:897	DMEM immersion	884:897	Their biodegradability upon DMEM immersion was followed up to seven days through measuring ionic concentrations of Ca, P, Ag and Pd successively.
29269259	2	6	theme	research	307:314	arg1	nanoparticles					279:291	Greenly synthesized noble metallic nanoparticles	244:291	Greenly synthesized noble metallic nanoparticles	244:291	Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine.
29269259	2	6	theme	research	307:314	arg1	area					316:319	a growing research area	297:319	a growing research area assessing their potential applications in nanomedicine	297:374	Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine.
29269259	4	7	theme	improved	572:579	arg1	activity					594:601	improved bactericidal activity	572:601	improved bactericidal activity	572:601	The developed composite scaffolds were functionalized with the greenly synthesized Ag or Ag@Pd targeting improved bactericidal activity and biocompatibility performance.
29269259	2	8	theme	potential	337:345	arg1	applications					347:358	their potential applications	331:358	their potential applications	331:358	Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine.
29269259	7	9	theme	Pd	985:986	arg1	concentrations					953:966	ionic concentrations	947:966	ionic concentrations of Ca, P, Ag and Pd successively	947:999	Their biodegradability upon DMEM immersion was followed up to seven days through measuring ionic concentrations of Ca, P, Ag and Pd successively.
29269259	7	10	dep	seven	918:922	arg1	to					915:916	to	915:916	to	915:916	Their biodegradability upon DMEM immersion was followed up to seven days through measuring ionic concentrations of Ca, P, Ag and Pd successively.
29269259	6	11	dep	aureus	807:812	arg1	aeruginosa					844:853	Pseudomonas aeruginosa	832:853	Pseudomonas aeruginosa	832:853	Bactericidal activities were tested against gram- positive Staphylococcus aureus and gram-negative Pseudomonas aeruginosa.
29269259	7	12	theme	Ag	978:979	arg1	concentrations					953:966	ionic concentrations	947:966	ionic concentrations of Ca, P, Ag and Pd successively	947:999	Their biodegradability upon DMEM immersion was followed up to seven days through measuring ionic concentrations of Ca, P, Ag and Pd successively.
29269259	4	13	theme	bactericidal	581:592	arg1	activity					594:601	improved bactericidal activity	572:601	improved bactericidal activity	572:601	The developed composite scaffolds were functionalized with the greenly synthesized Ag or Ag@Pd targeting improved bactericidal activity and biocompatibility performance.
29269259	9	14	theme	noble	1209:1213	arg1	incorporation					1222:1234	the noble metals incorporation	1205:1234	the noble metals incorporation	1205:1234	Further, their promising and differentiated bactericidal activities due to the noble metals incorporation were proved.
29269259	0	15	theme	Thermosensitive	0:14	arg1	chitosan/phosphate					16:33	Thermosensitive chitosan/phosphate	0:33	Thermosensitive chitosan/phosphate	0:33	Thermosensitive chitosan/phosphate hydrogel-composites fortified with Ag versus Ag@Pd for biomedical applications.
29269259	10	16	theme	toxic	1323:1327	arg1	effect					1329:1334	no toxic effect	1320:1334	no toxic effect	1320:1334	Cytotoxicity assessment demonstrated their high biocompatibility since no toxic effect was recorded.
29269259	4	17	theme	composite	481:489	arg1	scaffolds					491:499	The developed composite scaffolds	467:499	The developed composite scaffolds	467:499	The developed composite scaffolds were functionalized with the greenly synthesized Ag or Ag@Pd targeting improved bactericidal activity and biocompatibility performance.
29269259	7	18	theme	P	975:975	arg1	concentrations					953:966	ionic concentrations	947:966	ionic concentrations of Ca, P, Ag and Pd successively	947:999	Their biodegradability upon DMEM immersion was followed up to seven days through measuring ionic concentrations of Ca, P, Ag and Pd successively.
29269259	1	19	theme	temperature	223:233	arg1	changes					235:241	temperature changes	223:241	temperature changes	223:241	INTRODUCTION Thermo-responsive hydrogels are promising biomedical systems as their gelation is triggered by temperature changes.
29269259	4	20	theme	developed	471:479	arg1	scaffolds					491:499	The developed composite scaffolds	467:499	The developed composite scaffolds	467:499	The developed composite scaffolds were functionalized with the greenly synthesized Ag or Ag@Pd targeting improved bactericidal activity and biocompatibility performance.
29269259	1	21	theme	INTRODUCTION	115:126	arg1	hydrogels					146:154	INTRODUCTION Thermo-responsive hydrogels	115:154	INTRODUCTION Thermo-responsive hydrogels	115:154	INTRODUCTION Thermo-responsive hydrogels are promising biomedical systems as their gelation is triggered by temperature changes.
29269259	1	21	theme	INTRODUCTION	115:126	arg1	systems					181:187	promising biomedical systems	160:187	promising biomedical systems	160:187	INTRODUCTION Thermo-responsive hydrogels are promising biomedical systems as their gelation is triggered by temperature changes.
29269259	11	22	theme	biomedical	1426:1435	arg1	surgeries					1448:1456	biomedical and dental surgeries	1426:1456	biomedical and dental surgeries	1426:1456	SIGNIFICANCE Consequently, they can be successfully and directly applied in biomedical and dental surgeries.
29269259	4	23	theme	Ag	556:557	arg1	Pd					559:560	the greenly synthesized Ag or Ag@Pd	526:560	Pd	559:560	The developed composite scaffolds were functionalized with the greenly synthesized Ag or Ag@Pd targeting improved bactericidal activity and biocompatibility performance.
29269259	1	24	theme	Thermo-responsive	128:144	arg1	hydrogels					146:154	INTRODUCTION Thermo-responsive hydrogels	115:154	INTRODUCTION Thermo-responsive hydrogels	115:154	INTRODUCTION Thermo-responsive hydrogels are promising biomedical systems as their gelation is triggered by temperature changes.
29269259	1	24	theme	Thermo-responsive	128:144	arg1	systems					181:187	promising biomedical systems	160:187	promising biomedical systems	160:187	INTRODUCTION Thermo-responsive hydrogels are promising biomedical systems as their gelation is triggered by temperature changes.
29269259	7	25	theme	Ca	971:972	arg1	concentrations					953:966	ionic concentrations	947:966	ionic concentrations of Ca, P, Ag and Pd successively	947:999	Their biodegradability upon DMEM immersion was followed up to seven days through measuring ionic concentrations of Ca, P, Ag and Pd successively.
29269259	8	26	theme	developed	1025:1033	arg1	layers					1046:1051	The newly developed phosphatic layers	1015:1051	The newly developed phosphatic layers	1015:1051	KEY FINDINGS The newly developed phosphatic layers over the scaffold surfaces post-immersion assessed their osteogenic ability.
29269259	0	27	theme	@	82:82	arg1	Pd					83:84	Ag@Pd	80:84	Ag@Pd	80:84	Thermosensitive chitosan/phosphate hydrogel-composites fortified with Ag versus Ag@Pd for biomedical applications.
29269259	8	28	theme	phosphatic	1035:1044	arg1	layers					1046:1051	The newly developed phosphatic layers	1015:1051	The newly developed phosphatic layers	1015:1051	KEY FINDINGS The newly developed phosphatic layers over the scaffold surfaces post-immersion assessed their osteogenic ability.
29269259	9	29	theme	promising	1145:1153	arg1	activities					1187:1196	their promising and differentiated bactericidal activities	1139:1196	their promising and differentiated bactericidal activities due to the noble metals incorporation	1139:1234	Further, their promising and differentiated bactericidal activities due to the noble metals incorporation were proved.
29269259	10	30	theme	high	1292:1295	arg1	biocompatibility					1297:1312	their high biocompatibility	1286:1312	their high biocompatibility	1286:1312	Cytotoxicity assessment demonstrated their high biocompatibility since no toxic effect was recorded.
29269259	0	31	theme	Ag	80:81	arg1	Pd					83:84	Ag@Pd	80:84	Ag@Pd	80:84	Thermosensitive chitosan/phosphate hydrogel-composites fortified with Ag versus Ag@Pd for biomedical applications.
29269259	7	32	theme	ionic	947:951	arg1	concentrations					953:966	ionic concentrations	947:966	ionic concentrations of Ca, P, Ag and Pd successively	947:999	Their biodegradability upon DMEM immersion was followed up to seven days through measuring ionic concentrations of Ca, P, Ag and Pd successively.
29269259	4	33	theme	@	558:558	arg1	Pd					559:560	the greenly synthesized Ag or Ag@Pd	526:560	Pd	559:560	The developed composite scaffolds were functionalized with the greenly synthesized Ag or Ag@Pd targeting improved bactericidal activity and biocompatibility performance.
29269259	2	34	theme	metallic	270:277	arg1	nanoparticles					279:291	Greenly synthesized noble metallic nanoparticles	244:291	Greenly synthesized noble metallic nanoparticles	244:291	Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine.
29269259	2	34	theme	metallic	270:277	arg1	area					316:319	a growing research area	297:319	a growing research area assessing their potential applications in nanomedicine	297:374	Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine.
29269259	9	35	theme	differentiated	1159:1172	arg1	activities					1187:1196	their promising and differentiated bactericidal activities	1139:1196	their promising and differentiated bactericidal activities due to the noble metals incorporation	1139:1234	Further, their promising and differentiated bactericidal activities due to the noble metals incorporation were proved.
29269259	3	36	theme	MATERIALS	377:385	arg1	gels					434:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels	377:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels	377:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels were successfully achieved.
29269259	2	37	theme	synthesized	252:262	arg1	nanoparticles					279:291	Greenly synthesized noble metallic nanoparticles	244:291	Greenly synthesized noble metallic nanoparticles	244:291	Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine.
29269259	2	37	theme	synthesized	252:262	arg1	area					316:319	a growing research area	297:319	a growing research area assessing their potential applications in nanomedicine	297:374	Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine.
29269259	2	38	theme	noble	264:268	arg1	nanoparticles					279:291	Greenly synthesized noble metallic nanoparticles	244:291	Greenly synthesized noble metallic nanoparticles	244:291	Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine.
29269259	2	38	theme	noble	264:268	arg1	area					316:319	a growing research area	297:319	a growing research area assessing their potential applications in nanomedicine	297:374	Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine.
29269259	11	39	dep	Consequently	1363:1374	arg1	SIGNIFICANCE					1350:1361	SIGNIFICANCE	1350:1361	SIGNIFICANCE	1350:1361	SIGNIFICANCE Consequently, they can be successfully and directly applied in biomedical and dental surgeries.
29269259	9	40	theme	bactericidal	1174:1185	arg1	activities					1187:1196	their promising and differentiated bactericidal activities	1139:1196	their promising and differentiated bactericidal activities due to the noble metals incorporation	1139:1234	Further, their promising and differentiated bactericidal activities due to the noble metals incorporation were proved.
29269259	10	41	theme	Cytotoxicity	1249:1260	arg1	assessment					1262:1271	Cytotoxicity assessment	1249:1271	Cytotoxicity assessment	1249:1271	Cytotoxicity assessment demonstrated their high biocompatibility since no toxic effect was recorded.
29269259	8	42	theme	scaffold	1062:1069	arg1	surfaces					1071:1078	the scaffold surfaces	1058:1078	the scaffold surfaces	1058:1078	KEY FINDINGS The newly developed phosphatic layers over the scaffold surfaces post-immersion assessed their osteogenic ability.
29269259	1	43	theme	promising	160:168	arg1	hydrogels					146:154	INTRODUCTION Thermo-responsive hydrogels	115:154	INTRODUCTION Thermo-responsive hydrogels	115:154	INTRODUCTION Thermo-responsive hydrogels are promising biomedical systems as their gelation is triggered by temperature changes.
29269259	1	43	theme	promising	160:168	arg1	systems					181:187	promising biomedical systems	160:187	promising biomedical systems	160:187	INTRODUCTION Thermo-responsive hydrogels are promising biomedical systems as their gelation is triggered by temperature changes.
29269259	11	44	theme	dental	1441:1446	arg1	surgeries					1448:1456	biomedical and dental surgeries	1426:1456	biomedical and dental surgeries	1426:1456	SIGNIFICANCE Consequently, they can be successfully and directly applied in biomedical and dental surgeries.
29269259	0	45	dep	hydrogel-composites	35:53	arg1	fortified					55:63	fortified	55:63	hydrogel-composites fortified with Ag versus Ag@Pd for biomedical applications	35:112	Thermosensitive chitosan/phosphate hydrogel-composites fortified with Ag versus Ag@Pd for biomedical applications.
29269259	1	46	theme	biomedical	170:179	arg1	hydrogels					146:154	INTRODUCTION Thermo-responsive hydrogels	115:154	INTRODUCTION Thermo-responsive hydrogels	115:154	INTRODUCTION Thermo-responsive hydrogels are promising biomedical systems as their gelation is triggered by temperature changes.
29269259	1	46	theme	biomedical	170:179	arg1	systems					181:187	promising biomedical systems	160:187	promising biomedical systems	160:187	INTRODUCTION Thermo-responsive hydrogels are promising biomedical systems as their gelation is triggered by temperature changes.
29269259	3	47	theme	Chitosan/phosphate	399:416	arg1	gels					434:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels	377:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels	377:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels were successfully achieved.
29269259	4	48	theme	synthesized	538:548	arg1	Ag					550:551	the greenly synthesized Ag or Ag@Pd	526:560	Ag	550:551	The developed composite scaffolds were functionalized with the greenly synthesized Ag or Ag@Pd targeting improved bactericidal activity and biocompatibility performance.
29269259	2	49	theme	growing	299:305	arg1	nanoparticles					279:291	Greenly synthesized noble metallic nanoparticles	244:291	Greenly synthesized noble metallic nanoparticles	244:291	Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine.
29269259	2	49	theme	growing	299:305	arg1	area					316:319	a growing research area	297:319	a growing research area assessing their potential applications in nanomedicine	297:374	Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine.
29269259	5	50	theme	physicochemical	641:655	arg1	characterization					657:672	The physicochemical characterization	637:672	The physicochemical characterization	637:672	The physicochemical characterization was assessed through TGA, DSC, FESEM, HRTEM, XRD and FTIR.
29269259	8	51	theme	KEY	1002:1004	arg1	FINDINGS					1006:1013	KEY FINDINGS	1002:1013	KEY FINDINGS The newly developed phosphatic layers over the scaffold surfaces	1002:1078	KEY FINDINGS The newly developed phosphatic layers over the scaffold surfaces post-immersion assessed their osteogenic ability.
29269259	3	52	theme	thermosensitive	418:432	arg1	gels					434:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels	377:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels	377:437	MATERIALS AND METHODS Chitosan/phosphate thermosensitive gels were successfully achieved.
29269259	4	53	theme	biocompatibility	607:622	arg1	performance					624:634	biocompatibility performance	607:634	biocompatibility performance	607:634	The developed composite scaffolds were functionalized with the greenly synthesized Ag or Ag@Pd targeting improved bactericidal activity and biocompatibility performance.
29269259	8	54	theme	osteogenic	1110:1119	arg1	ability					1121:1127	their osteogenic ability	1104:1127	their osteogenic ability	1104:1127	KEY FINDINGS The newly developed phosphatic layers over the scaffold surfaces post-immersion assessed their osteogenic ability.
25186630	5	0	theme	bivariate	944:952	arg1	δ					980:980	δ(18)	980:984	δ(18)	980:984	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	0	theme	bivariate	944:952	arg1	C					971:971	the δ(13) C	961:971	the δ(13) C	961:971	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	0	theme	bivariate	944:952	arg1	plots					954:958	bivariate plots	944:958	bivariate plots	944:958	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	1	theme	natural	891:897	arg1	"					919:919	"biologics"	909:919	"biologics"	909:919	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	1	theme	natural	891:897	arg1	products					899:906	natural products	891:906	natural products ("biologics")	891:920	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	4	2	theme	discrimination	648:661	arg1	ability					663:669	the maximum discrimination ability	636:669	the maximum discrimination ability	636:669	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	5	3	theme	O	1154:1154	arg1	plot					1156:1159	δ(18) O plot	1148:1159	δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O	1148:1346	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	4	4	theme	bivariate	547:555	arg1	plot					557:560	a bivariate plot	545:560	a bivariate plot of δ(13) C and δ(18) O	545:583	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	4	4	theme	bivariate	547:555	arg1	plot					615:618	the best single, bivariate plot	588:618	the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set	588:750	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	0	5	theme	heparins	55:62	arg1	analysis					16:23	Stable isotopic analysis	0:23	Stable isotopic analysis of porcine, bovine, and ovine heparins.	0:63	Stable isotopic analysis of porcine, bovine, and ovine heparins.
25186630	4	6	theme	maximum	640:646	arg1	ability					663:669	the maximum discrimination ability	636:669	the maximum discrimination ability	636:669	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	5	7	dep	feasible	927:934	arg1	in					941:942	in	941:942	in	941:942	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	7	dep	feasible	927:934	arg1	3					1131:1131	3	1131:1131	3	1131:1131	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	7	dep	feasible	927:934	arg1	2					938:938	2	938:938	2	938:938	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	7	dep	feasible	927:934	arg1	reveals					993:999	reveals	993:999	reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China	993:1127	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	8	theme	well-defined	1179:1190	arg1	relationship					1192:1203	the most well-defined relationship	1170:1203	the most well-defined relationship	1170:1203	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	9	theme	food	1436:1439	arg1	sources					1441:1447	the food sources	1432:1447	the food sources used by the different heparin producers	1432:1487	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	4	10	theme	C	571:571	arg1	plot					557:560	a bivariate plot	545:560	a bivariate plot of δ(13) C and δ(18) O	545:583	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	4	10	theme	C	571:571	arg1	plot					615:618	the best single, bivariate plot	588:618	the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set	588:750	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	4	11	from	variation	726:734	arg1	set					748:750	the data set	739:750	the data set	739:750	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	5	12	theme	molecular	865:873	arg1	kDa					886:888	molecular weight ∼15 kDa	865:888	molecular weight ∼15 kDa	865:888	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	12	theme	molecular	865:873	arg1	polysaccharide					849:862	these highly sulfated polysaccharide	827:862	these highly sulfated polysaccharide (molecular weight ∼15 kDa)	827:889	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	13	theme	δ	1148:1148	arg1	plot					1156:1159	δ(18) O plot	1148:1159	δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O	1148:1346	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	14	dep	measurements	811:822	arg1	1					793:793	1	793:793	1	793:793	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	14	dep	measurements	811:822	arg1	feasible					927:934	feasible	927:934	feasible	927:934	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	14	dep	measurements	811:822	arg1	S					1358:1358	the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S	1134:1358	the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S	1134:1358	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	4	15	theme	δ	577:577	arg1	O					583:583	δ(18) O	577:583	δ(18) O	577:583	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	2	16	link	animal-derived	348:361	arg1	heparins					363:370	five different animal-derived heparins	333:370	five different animal-derived heparins	333:370	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	5	17	theme	δ	1325:1325	arg1	N					1331:1331	the δ(15) N	1321:1331	the δ(15) N	1321:1331	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	18	theme	weight	875:880	arg1	kDa					886:888	molecular weight ∼15 kDa	865:888	molecular weight ∼15 kDa	865:888	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	18	theme	weight	875:880	arg1	polysaccharide					849:862	these highly sulfated polysaccharide	827:862	these highly sulfated polysaccharide (molecular weight ∼15 kDa)	827:889	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	19	theme	source	1209:1214	arg1	differentiation					1216:1230	source differentiation	1209:1230	source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O	1209:1346	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	4	20	theme	Principle	504:512	arg1	analysis					524:531	Principle component analysis	504:531	Principle component analysis	504:531	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	2	21	theme	isotopic	308:315	arg1	compositions					317:328	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions	192:328	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins	192:370	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	5	22	theme	∼15	882:884	arg1	kDa					886:888	molecular weight ∼15 kDa	865:888	molecular weight ∼15 kDa	865:888	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	22	theme	∼15	882:884	arg1	polysaccharide					849:862	these highly sulfated polysaccharide	827:862	these highly sulfated polysaccharide (molecular weight ∼15 kDa)	827:889	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	23	theme	Stable	753:758	arg1	analyses					769:776	Stable isotopic analyses	753:776	Stable isotopic analyses	753:776	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	24	theme	environmental	1256:1268	arg1	isotopes					1270:1277	the hydrologic environmental isotopes	1241:1277	the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O	1241:1346	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	1	25	theme	pharmaceutical	141:154	arg1	industry					156:163	the pharmaceutical industry	137:163	the pharmaceutical industry	137:163	The assessment of provenance of heparin is becoming a major concern for the pharmaceutical industry and its regulatory bodies.
25186630	0	26	theme	isotopic	7:14	arg1	analysis					16:23	Stable isotopic analysis	0:23	Stable isotopic analysis of porcine, bovine, and ovine heparins.	0:63	Stable isotopic analysis of porcine, bovine, and ovine heparins.
25186630	2	27	theme	stable	301:306	arg1	compositions					317:328	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions	192:328	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins	192:370	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	2	28	dep	C	222:222	arg1	S					278:278	δ(34) S	272:278	δ(34) S	272:278	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	2	28	dep	C	222:222	arg1	δD					296:297	δD	296:297	δD	296:297	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	2	28	dep	C	222:222	arg1	sulfur					264:269	sulfur	264:269	sulfur (δ(34) S)	264:279	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	2	28	dep	C	222:222	arg1	nitrogen					226:233	nitrogen	226:233	nitrogen (δ(15) N)	226:243	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	2	28	dep	C	222:222	arg1	N					242:242	δ(15) N	236:242	δ(15) N	236:242	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	2	28	dep	C	222:222	arg1	oxygen					246:251	oxygen	246:251	oxygen (δ(18) O)	246:261	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	2	28	dep	C	222:222	arg1	hydrogen					286:293	hydrogen	286:293	hydrogen (δD)	286:298	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	2	28	dep	C	222:222	arg1	O					260:260	δ(18) O	254:260	δ(18) O	254:260	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	0	29	theme	Stable	0:5	arg1	analysis					16:23	Stable isotopic analysis	0:23	Stable isotopic analysis of porcine, bovine, and ovine heparins.	0:63	Stable isotopic analysis of porcine, bovine, and ovine heparins.
25186630	5	30	theme	different	1461:1469	arg1	producers					1479:1487	the different heparin producers	1457:1487	the different heparin producers	1457:1487	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	31	theme	sulfated	840:847	arg1	kDa					886:888	molecular weight ∼15 kDa	865:888	molecular weight ∼15 kDa	865:888	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	31	theme	sulfated	840:847	arg1	polysaccharide					849:862	these highly sulfated polysaccharide	827:862	these highly sulfated polysaccharide (molecular weight ∼15 kDa)	827:889	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	4	32	theme	component	514:522	arg1	analysis					524:531	Principle component analysis	504:531	Principle component analysis	504:531	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	5	33	theme	D/H	1289:1291	arg1	water					1282:1286	water	1282:1286	water	1282:1286	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	33	theme	D/H	1289:1291	arg1	O					1309:1309	D/H and (18) O/(16) O	1289:1309	O	1309:1309	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	34	theme	heparin	1471:1477	arg1	producers					1479:1487	the different heparin producers	1457:1487	the different heparin producers	1457:1487	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	35	theme	plot	1156:1159	arg1	S					1358:1358	the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S	1134:1358	the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S	1134:1358	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	36	theme	water	1282:1286	arg1	isotopes					1270:1277	the hydrologic environmental isotopes	1241:1277	the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O	1241:1346	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	37	theme	O	986:986	arg1	plot					988:991	bivariate plots, the δ(13) C versus δ(18) O plot	944:991	bivariate plots, the δ(13) C versus δ(18) O plot	944:991	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	38	theme	N	1331:1331	arg1	isotopes					1270:1277	the hydrologic environmental isotopes	1241:1277	the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O	1241:1346	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	2	39	theme	heparins	363:370	arg1	compositions					317:328	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions	192:328	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins	192:370	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	3	40	theme	isotopic	483:490	arg1	provenance					492:501	their isotopic provenance	477:501	their isotopic provenance	477:501	Measurements readily allowed their differentiation into groups and/or subgroups based on their isotopic provenance.
25186630	1	41	theme	provenance	83:92	arg1	assessment					69:78	The assessment	65:78	The assessment of provenance of heparin	65:103	The assessment of provenance of heparin is becoming a major concern for the pharmaceutical industry and its regulatory bodies.
25186630	1	42	theme	regulatory	173:182	arg1	bodies					184:189	its regulatory bodies	169:189	its regulatory bodies	169:189	The assessment of provenance of heparin is becoming a major concern for the pharmaceutical industry and its regulatory bodies.
25186630	5	43	theme	hydrologic	1245:1254	arg1	isotopes					1270:1277	the hydrologic environmental isotopes	1241:1277	the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O	1241:1346	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	44	theme	well-defined	1003:1014	arg1	relationship					1016:1027	a well-defined relationship	1001:1027	a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China	1001:1127	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	2	45	theme	animal-derived	348:361	arg1	heparins					363:370	five different animal-derived heparins	333:370	five different animal-derived heparins	333:370	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	5	46	theme	O	1373:1373	arg1	relationships					1375:1387	δ(18) O relationships	1367:1387	δ(18) O relationships	1367:1387	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	47	theme	stable	796:801	arg1	measurements					811:822	(1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships	792:1387	measurements	811:822	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	0	48	theme	porcine	28:34	arg1	analysis					16:23	Stable isotopic analysis	0:23	Stable isotopic analysis of porcine, bovine, and ovine heparins.	0:63	Stable isotopic analysis of porcine, bovine, and ovine heparins.
25186630	2	49	theme	different	338:346	arg1	heparins					363:370	five different animal-derived heparins	333:370	five different animal-derived heparins	333:370	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	4	50	theme	single	597:602	arg1	plot					557:560	a bivariate plot	545:560	a bivariate plot of δ(13) C and δ(18) O	545:583	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	4	50	theme	single	597:602	arg1	plot					615:618	the best single, bivariate plot	588:618	the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set	588:750	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	4	51	dep	single	597:602	arg1	bivariate					605:613	bivariate	605:613	bivariate	605:613	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	5	52	theme	biologics	910:918	arg1	"					919:919	"biologics"	909:919	"biologics"	909:919	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	52	theme	biologics	910:918	arg1	products					899:906	natural products	891:906	natural products ("biologics")	891:920	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	2	53	theme	[carbon	207:213	arg1	compositions					317:328	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions	192:328	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins	192:370	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	5	54	from	hogs	1097:1100	arg1	States					1085:1090	the United States	1074:1090	the United States from hogs raised in Europe and China	1074:1127	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	0	55	theme	bovine	37:42	arg1	analysis					16:23	Stable isotopic analysis	0:23	Stable isotopic analysis of porcine, bovine, and ovine heparins.	0:63	Stable isotopic analysis of porcine, bovine, and ovine heparins.
25186630	5	56	theme	isotope	803:809	arg1	measurements					811:822	(1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships	792:1387	measurements	811:822	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	2	57	theme	Batch-specific	192:205	arg1	[carbon					207:213	Batch-specific [carbon	192:213	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins	192:370	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	2	57	theme	Batch-specific	192:205	arg1	C					222:222	δ(13) C	216:222	δ(13) C	216:222	Batch-specific [carbon (δ(13) C), nitrogen (δ(15) N), oxygen (δ(18) O), sulfur (δ(34) S), and hydrogen (δD)] stable isotopic compositions of five different animal-derived heparins were performed.
25186630	5	58	theme	δD	1138:1139	arg1	S					1358:1358	the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S	1134:1358	the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S	1134:1358	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	1	59	theme	heparin	97:103	arg1	provenance					83:92	provenance	83:92	provenance of heparin	83:103	The assessment of provenance of heparin is becoming a major concern for the pharmaceutical industry and its regulatory bodies.
25186630	5	60	theme	δ	1352:1352	arg1	S					1358:1358	the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S	1134:1358	the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S	1134:1358	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	61	theme	δ	1367:1367	arg1	relationships					1375:1387	δ(18) O relationships	1367:1387	δ(18) O relationships	1367:1387	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	62	theme	isotopic	760:767	arg1	analyses					769:776	Stable isotopic analyses	753:776	Stable isotopic analyses	753:776	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	63	theme	United	1078:1083	arg1	States					1085:1090	the United States	1074:1090	the United States from hogs raised in Europe and China	1074:1127	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	64	theme	hogs	1059:1062	arg1	differentiation					1040:1054	source differentiation	1033:1054	source differentiation of hogs raised in the United States from hogs raised in Europe and China	1033:1127	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	65	dep	N	1331:1331	arg1	4					1318:1318	4	1318:1318	4	1318:1318	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	66	theme	plots	954:958	arg1	plot					988:991	bivariate plots, the δ(13) C versus δ(18) O plot	944:991	bivariate plots, the δ(13) C versus δ(18) O plot	944:991	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	4	67	theme	stable	685:690	arg1	isotopes					692:699	only two stable isotopes	676:699	only two stable isotopes	676:699	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	4	68	theme	O	583:583	arg1	plot					557:560	a bivariate plot	545:560	a bivariate plot of δ(13) C and δ(18) O	545:583	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	4	68	theme	O	583:583	arg1	plot					615:618	the best single, bivariate plot	588:618	the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set	588:750	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	5	69	theme	source	1033:1038	arg1	differentiation					1040:1054	source differentiation	1033:1054	source differentiation of hogs raised in the United States from hogs raised in Europe and China	1033:1127	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	4	70	theme	δ	565:565	arg1	C					571:571	δ(13) C	565:571	δ(13) C	565:571	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	0	71	theme	ovine	49:53	arg1	heparins					55:62	ovine heparins	49:62	ovine heparins	49:62	Stable isotopic analysis of porcine, bovine, and ovine heparins.
25186630	5	72	theme	O/	1302:1303	arg1	water					1282:1286	water	1282:1286	water	1282:1286	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	72	theme	O/	1302:1303	arg1	O					1309:1309	D/H and (18) O/(16) O	1289:1309	O	1309:1309	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	73	theme	δ	1340:1340	arg1	O					1346:1346	δ(18) O	1340:1346	δ(18) O	1340:1346	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	4	74	theme	data	743:746	arg1	set					748:750	the data set	739:750	the data set	739:750	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25186630	5	75	theme	δ	965:965	arg1	C					971:971	the δ(13) C	961:971	the δ(13) C	961:971	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	5	75	theme	δ	965:965	arg1	plots					954:958	bivariate plots	944:958	bivariate plots	944:958	Stable isotopic analyses revealed that (1) stable isotope measurements on these highly sulfated polysaccharide (molecular weight ∼15 kDa) natural products ("biologics") were feasible; (2) in bivariate plots, the δ(13) C versus δ(18) O plot reveals a well-defined relationship for source differentiation of hogs raised in the United States from hogs raised in Europe and China; (3) the δD versus δ(18) O plot revealed the most well-defined relationship for source differentiation based on the hydrologic environmental isotopes of water (D/H and (18) O/(16) O); and (4) the δ(15) N versus δ(18) O and δ(34) S versus δ(18) O relationships are both very similar, possibly reflecting the food sources used by the different heparin producers.
25186630	1	76	theme	major	119:123	arg1	concern					125:131	a major concern	117:131	a major concern for the pharmaceutical industry and its regulatory bodies	117:189	The assessment of provenance of heparin is becoming a major concern for the pharmaceutical industry and its regulatory bodies.
25186630	4	77	used	used	705:708	arg2	isotopes					692:699	only two stable isotopes	676:699	only two stable isotopes	676:699	Principle component analysis showed that a bivariate plot of δ(13) C and δ(18) O is the best single, bivariate plot that results in the maximum discrimination ability when only two stable isotopes are used to describe the variation in the data set.
25498726	6	0	theme	Shear	814:818	arg1	results					832:838	Shear viscosities results	814:838	Shear viscosities results	814:838	Shear viscosities results indicated improved compatibilization as the organoclay content was increased.
25498726	1	1	theme	m/m	210:212	arg1	composition					215:225	a constant 70:30 (m/m) composition	192:225	a constant 70:30 (m/m) composition	192:225	Glycerol-plasticized cornstarch and poly(lactic acid) (PLA) were melt-blended alone and at a constant 70:30 (m/m) composition, in the present of an organoclay.
25498726	2	2	theme	thermogravimetric	419:435	arg1	TGA					447:449	TGA	447:449	TGA	447:449	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	2	2	theme	thermogravimetric	419:435	arg1	analysis					437:444	thermogravimetric analysis	419:444	thermogravimetric analysis (TGA)	419:450	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	3	3	dep	components	564:573	arg1	components					564:573	the hybrid components	553:573	the hybrid components (TPS/organoclay and PLA/organoclay)	553:609	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay) were correlated with the hybrid blends properties.
25498726	3	3	dep	components	564:573	arg1	PLA/organoclay					595:608	PLA/organoclay	595:608	PLA/organoclay	595:608	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay) were correlated with the hybrid blends properties.
25498726	3	3	dep	components	564:573	arg1	TPS/organoclay					576:589	TPS/organoclay	576:589	TPS/organoclay	576:589	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay) were correlated with the hybrid blends properties.
25498726	0	4	theme	hybrid	86:91	arg1	blends					93:98	starch-rich hybrid blends	74:98	starch-rich hybrid blends	74:98	Effect of structure and viscosity of the components on some properties of starch-rich hybrid blends.
25498726	0	5	from	Effect	0:5	arg1	properties					60:69	some properties	55:69	some properties of starch-rich hybrid blends	55:98	Effect of structure and viscosity of the components on some properties of starch-rich hybrid blends.
25498726	2	6	from	effect	265:270	arg1	samples					348:354	extruded and compression-molded samples	316:354	extruded and compression-molded samples	316:354	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	2	7	theme	scanning	453:460	arg1	SEM					483:485	SEM	483:485	SEM	483:485	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	2	7	theme	scanning	453:460	arg1	microscopy					471:480	scanning electron microscopy	453:480	scanning electron microscopy (SEM)	453:486	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	2	8	theme	contents	286:293	arg1	effect					265:270	The effect	261:270	The effect of increasing contents of the organoclay on extruded and compression-molded samples	261:354	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	8	9	theme	higher	1153:1158	arg1	contents					1160:1167	higher contents	1153:1167	higher contents of the organoclay	1153:1185	For the extruded and compression-molded samples, improved mechanical properties were obtained for the samples with higher contents of the organoclay.
25498726	0	10	theme	blends	93:98	arg1	properties					60:69	some properties	55:69	some properties of starch-rich hybrid blends	55:98	Effect of structure and viscosity of the components on some properties of starch-rich hybrid blends.
25498726	3	11	theme	hybrid	636:641	arg1	properties					650:659	the hybrid blends properties	632:659	the hybrid blends properties	632:659	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay) were correlated with the hybrid blends properties.
25498726	9	12	theme	injection-molded	1196:1211	arg1	samples					1213:1219	the injection-molded samples	1192:1219	the injection-molded samples	1192:1219	For the injection-molded samples, the mechanical properties seemed to be dependent on the organoclay dispersion.
25498726	2	13	theme	compression-molded	329:346	arg1	samples					348:354	extruded and compression-molded samples	316:354	extruded and compression-molded samples	316:354	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	3	14	theme	blends	643:648	arg1	properties					650:659	the hybrid blends properties	632:659	the hybrid blends properties	632:659	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay) were correlated with the hybrid blends properties.
25498726	8	15	theme	extruded	1046:1053	arg1	samples					1078:1084	the extruded and compression-molded samples	1042:1084	the extruded and compression-molded samples	1042:1084	For the extruded and compression-molded samples, improved mechanical properties were obtained for the samples with higher contents of the organoclay.
25498726	4	16	theme	XRD	662:664	arg1	results					674:680	XRD and TGA results	662:680	XRD and TGA results	662:680	XRD and TGA results suggested that the organoclay was similarly dispersed within both phases.
25498726	2	17	theme	extruded	316:323	arg1	samples					348:354	extruded and compression-molded samples	316:354	extruded and compression-molded samples	316:354	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	8	18	theme	organoclay	1176:1185	arg1	contents					1160:1167	higher contents	1153:1167	higher contents of the organoclay	1153:1185	For the extruded and compression-molded samples, improved mechanical properties were obtained for the samples with higher contents of the organoclay.
25498726	2	19	theme	electron	462:469	arg1	SEM					483:485	SEM	483:485	SEM	483:485	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	2	19	theme	electron	462:469	arg1	microscopy					471:480	scanning electron microscopy	453:480	scanning electron microscopy (SEM)	453:486	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	0	20	theme	structure	10:18	arg1	Effect					0:5	Effect	0:5	Effect of structure and viscosity of the components on some properties of starch-rich hybrid blends.	0:99	Effect of structure and viscosity of the components on some properties of starch-rich hybrid blends.
25498726	7	21	theme	tensile	1023:1029	arg1	tests					1031:1035	tensile tests	1023:1035	tensile tests	1023:1035	Some of the extruded materials were also submitted to injection molding, and characterized by SEM and by tensile tests.
25498726	4	22	theme	TGA	670:672	arg1	results					674:680	XRD and TGA results	662:680	XRD and TGA results	662:680	XRD and TGA results suggested that the organoclay was similarly dispersed within both phases.
25498726	9	23	theme	organoclay	1278:1287	arg1	dispersion					1289:1298	the organoclay dispersion	1274:1298	the organoclay dispersion	1274:1298	For the injection-molded samples, the mechanical properties seemed to be dependent on the organoclay dispersion.
25498726	5	24	theme	improved	776:783	arg1	adhesion					785:792	improved adhesion	776:792	improved adhesion between the phases	776:811	SEM images revealed improved adhesion between the phases.
25498726	0	25	theme	viscosity	24:32	arg1	Effect					0:5	Effect	0:5	Effect of structure and viscosity of the components on some properties of starch-rich hybrid blends.	0:99	Effect of structure and viscosity of the components on some properties of starch-rich hybrid blends.
25498726	8	26	theme	mechanical	1096:1105	arg1	properties					1107:1116	improved mechanical properties	1087:1116	improved mechanical properties	1087:1116	For the extruded and compression-molded samples, improved mechanical properties were obtained for the samples with higher contents of the organoclay.
25498726	3	27	theme	shear	516:520	arg1	viscosity					522:530	shear viscosity	516:530	shear viscosity	516:530	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay) were correlated with the hybrid blends properties.
25498726	7	28	theme	injection	972:980	arg1	molding					982:988	injection molding	972:988	injection molding	972:988	Some of the extruded materials were also submitted to injection molding, and characterized by SEM and by tensile tests.
25498726	0	29	theme	components	41:50	arg1	structure					10:18	structure	10:18	structure	10:18	Effect of structure and viscosity of the components on some properties of starch-rich hybrid blends.
25498726	0	29	theme	components	41:50	arg1	viscosity					24:32	viscosity	24:32	viscosity	24:32	Effect of structure and viscosity of the components on some properties of starch-rich hybrid blends.
25498726	1	30	theme	organoclay	249:258	arg1	present					235:241	the present	231:241	the present of an organoclay	231:258	Glycerol-plasticized cornstarch and poly(lactic acid) (PLA) were melt-blended alone and at a constant 70:30 (m/m) composition, in the present of an organoclay.
25498726	3	31	theme	hybrid	557:562	arg1	components					564:573	the hybrid components	553:573	the hybrid components (TPS/organoclay and PLA/organoclay)	553:609	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay) were correlated with the hybrid blends properties.
25498726	3	31	theme	hybrid	557:562	arg1	PLA/organoclay					595:608	PLA/organoclay	595:608	PLA/organoclay	595:608	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay) were correlated with the hybrid blends properties.
25498726	3	31	theme	hybrid	557:562	arg1	TPS/organoclay					576:589	TPS/organoclay	576:589	TPS/organoclay	576:589	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay) were correlated with the hybrid blends properties.
25498726	6	32	theme	viscosities	820:830	arg1	results					832:838	Shear viscosities results	814:838	Shear viscosities results	814:838	Shear viscosities results indicated improved compatibilization as the organoclay content was increased.
25498726	1	33	theme	Glycerol-plasticized	101:120	arg1	cornstarch					122:131	Glycerol-plasticized cornstarch	101:131	Glycerol-plasticized cornstarch	101:131	Glycerol-plasticized cornstarch and poly(lactic acid) (PLA) were melt-blended alone and at a constant 70:30 (m/m) composition, in the present of an organoclay.
25498726	6	34	theme	improved	850:857	arg1	compatibilization					859:875	improved compatibilization	850:875	improved compatibilization	850:875	Shear viscosities results indicated improved compatibilization as the organoclay content was increased.
25498726	2	35	theme	tensile	493:499	arg1	tests					501:505	tensile tests	493:505	tensile tests	493:505	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	3	36	theme	XRD	508:510	arg1	results					532:538	XRD and shear viscosity results	508:538	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay)	508:609	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay) were correlated with the hybrid blends properties.
25498726	2	37	theme	increasing	275:284	arg1	contents					286:293	increasing contents	275:293	increasing contents of the organoclay	275:311	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	6	38	theme	organoclay	884:893	arg1	content					895:901	the organoclay content	880:901	the organoclay content	880:901	Shear viscosities results indicated improved compatibilization as the organoclay content was increased.
25498726	8	39	with	samples	1140:1146	arg1	contents					1160:1167	higher contents	1153:1167	higher contents of the organoclay	1153:1185	For the extruded and compression-molded samples, improved mechanical properties were obtained for the samples with higher contents of the organoclay.
25498726	8	40	theme	compression-molded	1059:1076	arg1	samples					1078:1084	the extruded and compression-molded samples	1042:1084	the extruded and compression-molded samples	1042:1084	For the extruded and compression-molded samples, improved mechanical properties were obtained for the samples with higher contents of the organoclay.
25498726	9	41	theme	mechanical	1226:1235	arg1	dependent					1261:1269	dependent	1261:1269	dependent	1261:1269	For the injection-molded samples, the mechanical properties seemed to be dependent on the organoclay dispersion.
25498726	9	41	theme	mechanical	1226:1235	arg1	properties					1237:1246	the mechanical properties	1222:1246	the mechanical properties	1222:1246	For the injection-molded samples, the mechanical properties seemed to be dependent on the organoclay dispersion.
25498726	1	42	theme	lactic	142:147	arg1	poly					137:140	poly	137:140	poly(lactic acid) (PLA)	137:159	Glycerol-plasticized cornstarch and poly(lactic acid) (PLA) were melt-blended alone and at a constant 70:30 (m/m) composition, in the present of an organoclay.
25498726	1	42	theme	lactic	142:147	arg1	acid					149:152	lactic acid	142:152	lactic acid	142:152	Glycerol-plasticized cornstarch and poly(lactic acid) (PLA) were melt-blended alone and at a constant 70:30 (m/m) composition, in the present of an organoclay.
25498726	1	43	theme	constant	194:201	arg1	composition					215:225	a constant 70:30 (m/m) composition	192:225	a constant 70:30 (m/m) composition	192:225	Glycerol-plasticized cornstarch and poly(lactic acid) (PLA) were melt-blended alone and at a constant 70:30 (m/m) composition, in the present of an organoclay.
25498726	8	44	theme	improved	1087:1094	arg1	properties					1107:1116	improved mechanical properties	1087:1116	improved mechanical properties	1087:1116	For the extruded and compression-molded samples, improved mechanical properties were obtained for the samples with higher contents of the organoclay.
25498726	3	45	theme	viscosity	522:530	arg1	results					532:538	XRD and shear viscosity results	508:538	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay)	508:609	XRD and shear viscosity results obtained for the hybrid components (TPS/organoclay and PLA/organoclay) were correlated with the hybrid blends properties.
25498726	2	46	theme	X-ray	373:377	arg1	XRD					392:394	XRD	392:394	XRD	392:394	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	2	46	theme	X-ray	373:377	arg1	diffraction					379:389	X-ray diffraction	373:389	X-ray diffraction (XRD)	373:395	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	1	47	theme	70:30	203:207	arg1	composition					215:225	a constant 70:30 (m/m) composition	192:225	a constant 70:30 (m/m) composition	192:225	Glycerol-plasticized cornstarch and poly(lactic acid) (PLA) were melt-blended alone and at a constant 70:30 (m/m) composition, in the present of an organoclay.
25498726	2	48	theme	capillary	398:406	arg1	rheometry					408:416	capillary rheometry	398:416	capillary rheometry	398:416	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	5	49	theme	SEM	756:758	arg1	images					760:765	SEM images	756:765	SEM images	756:765	SEM images revealed improved adhesion between the phases.
25498726	7	50	theme	extruded	930:937	arg1	materials					939:947	the extruded materials	926:947	the extruded materials	926:947	Some of the extruded materials were also submitted to injection molding, and characterized by SEM and by tensile tests.
25498726	2	51	theme	organoclay	302:311	arg1	contents					286:293	increasing contents	275:293	increasing contents of the organoclay	275:311	The effect of increasing contents of the organoclay on extruded and compression-molded samples was evaluated by X-ray diffraction (XRD), capillary rheometry, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile tests.
25498726	0	52	theme	starch-rich	74:84	arg1	blends					93:98	starch-rich hybrid blends	74:98	starch-rich hybrid blends	74:98	Effect of structure and viscosity of the components on some properties of starch-rich hybrid blends.
29102790	0	0	theme	acid	104:107	arg1	degradation					109:119	H2O2/ascorbic acid degradation	90:119	H2O2/ascorbic acid degradation	90:119	Preparation and antioxidant properties of low molecular holothurian glycosaminoglycans by H2O2/ascorbic acid degradation.
29102790	5	1	theme	sulfate	824:830	arg1	backbone					832:839	chondroitin sulfate backbone	812:839	chondroitin sulfate backbone	812:839	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	5	2	theme	low	754:756	arg1	product					768:774	a low molecular product	752:774	a low molecular product (DHmG-2)	752:783	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	5	2	theme	low	754:756	arg1	DHmG-2					777:782	DHmG-2	777:782	DHmG-2	777:782	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	6	3	theme	scavenging	936:945	arg1	abilities					947:955	a fine scavenging abilities	929:955	a fine scavenging abilities	929:955	The antioxidant assays of HmG and DHmG-3 showed a fine scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals in concentration dependent manner.
29102790	7	4	theme	antioxidant	1121:1131	arg1	activities					1133:1142	the antioxidant activities	1117:1142	the antioxidant activities of HmG	1117:1149	Besides, molecular weight did not obviously affect the antioxidant activities of HmG.
29102790	0	5	theme	H2O2/ascorbic	90:102	arg1	degradation					109:119	H2O2/ascorbic acid degradation	90:119	H2O2/ascorbic acid degradation	90:119	Preparation and antioxidant properties of low molecular holothurian glycosaminoglycans by H2O2/ascorbic acid degradation.
29102790	5	6	theme	dimension	712:720	arg1	resonance					739:747	Two dimension nuclear magnetic resonance	708:747	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2)	708:783	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	6	7	theme	antioxidant	885:895	arg1	assays					897:902	The antioxidant assays	881:902	The antioxidant assays of HmG and DHmG-3	881:920	The antioxidant assays of HmG and DHmG-3 showed a fine scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals in concentration dependent manner.
29102790	1	8	theme	H2O2/ascorbic	181:193	arg1	acid					195:198	H2O2/ascorbic acid	181:198	H2O2/ascorbic acid	181:198	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG).
29102790	4	9	theme	primary	628:634	arg1	structure					636:644	the primary structure	624:644	the primary structure	624:644	The results showed that the primary structure and sulfate esters were well reserved during the degradation.
29102790	5	10	contain	contained	802:810	arg2	backbone					832:839	chondroitin sulfate backbone	812:839	chondroitin sulfate backbone	812:839	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	5	10	contain	contained	802:810	arg1	it					799:800	it	799:800	it	799:800	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	5	10	contain	contained	802:810	arg2	branches					871:878	major 4-O-sulfated fucose branches	845:878	major 4-O-sulfated fucose branches	845:878	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	1	11	from	mexicana	336:343	arg1	glycosaminoglycan					302:318	a novel glycosaminoglycan	294:318	a novel glycosaminoglycan from Holothuria mexicana (HmG)	294:349	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG).
29102790	4	12	theme	sulfate	650:656	arg1	esters					658:663	sulfate esters	650:663	sulfate esters	650:663	The results showed that the primary structure and sulfate esters were well reserved during the degradation.
29102790	5	13	theme	magnetic	730:737	arg1	resonance					739:747	Two dimension nuclear magnetic resonance	708:747	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2)	708:783	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	3	14	dep	composition	532:542	arg1	The					519:521	The	519:521	The	519:521	The chemical composition and structure of different molecular HmG were analyzed.
29102790	2	15	theme	reduced	425:431	arg1	concentration					442:454	the reduced solution concentration	421:454	the reduced solution concentration	421:454	The results indicated that the degradation efficiency increased with the reduced solution concentration, increased H2O2 concentration and ascorbic acid concentration.
29102790	0	16	theme	molecular	46:54	arg1	glycosaminoglycans					68:85	low molecular holothurian glycosaminoglycans	42:85	low molecular holothurian glycosaminoglycans	42:85	Preparation and antioxidant properties of low molecular holothurian glycosaminoglycans by H2O2/ascorbic acid degradation.
29102790	6	17	theme	DHmG-3	915:920	arg1	assays					897:902	The antioxidant assays	881:902	The antioxidant assays of HmG and DHmG-3	881:920	The antioxidant assays of HmG and DHmG-3 showed a fine scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals in concentration dependent manner.
29102790	2	18	theme	ascorbic	490:497	arg1	concentration					504:516	ascorbic acid concentration	490:516	ascorbic acid concentration	490:516	The results indicated that the degradation efficiency increased with the reduced solution concentration, increased H2O2 concentration and ascorbic acid concentration.
29102790	3	19	theme	HmG	581:583	arg1	structure					548:556	structure	548:556	structure	548:556	The chemical composition and structure of different molecular HmG were analyzed.
29102790	3	19	theme	HmG	581:583	arg1	composition					532:542	chemical composition	523:542	chemical composition	523:542	The chemical composition and structure of different molecular HmG were analyzed.
29102790	1	20	theme	novel	296:300	arg1	glycosaminoglycan					302:318	a novel glycosaminoglycan	294:318	a novel glycosaminoglycan from Holothuria mexicana (HmG)	294:349	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG).
29102790	6	21	theme	dependent	1048:1056	arg1	manner					1058:1063	concentration dependent manner	1034:1063	concentration dependent manner	1034:1063	The antioxidant assays of HmG and DHmG-3 showed a fine scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals in concentration dependent manner.
29102790	5	22	theme	molecular	758:766	arg1	product					768:774	a low molecular product	752:774	a low molecular product (DHmG-2)	752:783	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	5	22	theme	molecular	758:766	arg1	DHmG-2					777:782	DHmG-2	777:782	DHmG-2	777:782	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	5	23	theme	4-O-sulfated	851:862	arg1	branches					871:878	major 4-O-sulfated fucose branches	845:878	major 4-O-sulfated fucose branches	845:878	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	2	24	theme	increased	457:465	arg1	concentration					472:484	increased H2O2 concentration	457:484	increased H2O2 concentration	457:484	The results indicated that the degradation efficiency increased with the reduced solution concentration, increased H2O2 concentration and ascorbic acid concentration.
29102790	2	25	theme	H2O2	467:470	arg1	concentration					472:484	increased H2O2 concentration	457:484	increased H2O2 concentration	457:484	The results indicated that the degradation efficiency increased with the reduced solution concentration, increased H2O2 concentration and ascorbic acid concentration.
29102790	5	26	theme	product	768:774	arg1	resonance					739:747	Two dimension nuclear magnetic resonance	708:747	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2)	708:783	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	6	27	theme	fine	931:934	arg1	scavenging					936:945	a fine scavenging	929:945	a fine scavenging abilities	929:955	The antioxidant assays of HmG and DHmG-3 showed a fine scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals in concentration dependent manner.
29102790	1	28	theme	effective	125:133	arg1	method					135:140	An effective method	122:140	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method,	122:262	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG).
29102790	3	29	theme	molecular	571:579	arg1	HmG					581:583	different molecular HmG	561:583	different molecular HmG	561:583	The chemical composition and structure of different molecular HmG were analyzed.
29102790	0	30	theme	low	42:44	arg1	glycosaminoglycans					68:85	low molecular holothurian glycosaminoglycans	42:85	low molecular holothurian glycosaminoglycans	42:85	Preparation and antioxidant properties of low molecular holothurian glycosaminoglycans by H2O2/ascorbic acid degradation.
29102790	5	31	theme	major	845:849	arg1	branches					871:878	major 4-O-sulfated fucose branches	845:878	major 4-O-sulfated fucose branches	845:878	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	7	32	theme	HmG	1147:1149	arg1	activities					1133:1142	the antioxidant activities	1117:1142	the antioxidant activities of HmG	1117:1149	Besides, molecular weight did not obviously affect the antioxidant activities of HmG.
29102790	1	33	theme	reported	237:244	arg1	method					256:261	previously reported H2O2/Cu2+ method	226:261	previously reported H2O2/Cu2+ method	226:261	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG).
29102790	0	34	theme	holothurian	56:66	arg1	glycosaminoglycans					68:85	low molecular holothurian glycosaminoglycans	42:85	low molecular holothurian glycosaminoglycans	42:85	Preparation and antioxidant properties of low molecular holothurian glycosaminoglycans by H2O2/ascorbic acid degradation.
29102790	6	35	theme	hydroxyl	1013:1020	arg1	radicals					1022:1029	hydroxyl radicals	1013:1029	hydroxyl radicals	1013:1029	The antioxidant assays of HmG and DHmG-3 showed a fine scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals in concentration dependent manner.
29102790	5	36	theme	fucose	864:869	arg1	branches					871:878	major 4-O-sulfated fucose branches	845:878	major 4-O-sulfated fucose branches	845:878	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	5	37	theme	chondroitin	812:822	arg1	backbone					832:839	chondroitin sulfate backbone	812:839	chondroitin sulfate backbone	812:839	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	2	38	theme	acid	499:502	arg1	concentration					504:516	ascorbic acid concentration	490:516	ascorbic acid concentration	490:516	The results indicated that the degradation efficiency increased with the reduced solution concentration, increased H2O2 concentration and ascorbic acid concentration.
29102790	3	39	theme	different	561:569	arg1	HmG					581:583	different molecular HmG	561:583	different molecular HmG	561:583	The chemical composition and structure of different molecular HmG were analyzed.
29102790	5	40	theme	nuclear	722:728	arg1	resonance					739:747	Two dimension nuclear magnetic resonance	708:747	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2)	708:783	Two dimension nuclear magnetic resonance of a low molecular product (DHmG-2) indicate that it contained chondroitin sulfate backbone and major 4-O-sulfated fucose branches.
29102790	6	41	theme	HmG	907:909	arg1	assays					897:902	The antioxidant assays	881:902	The antioxidant assays of HmG and DHmG-3	881:920	The antioxidant assays of HmG and DHmG-3 showed a fine scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals in concentration dependent manner.
29102790	1	42	theme	free	145:148	arg1	radical					150:156	free radical	145:156	free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method,	145:262	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG).
29102790	1	43	theme	H2O2/Cu2+	246:254	arg1	method					256:261	previously reported H2O2/Cu2+ method	226:261	previously reported H2O2/Cu2+ method	226:261	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG).
29102790	1	44	from	method	256:261	arg1	different					211:219	different	211:219	different	211:219	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG).
29102790	1	45	theme	radical	150:156	arg1	degradation					158:168	free radical degradation	145:168	free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method,	145:262	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG).
29102790	3	46	theme	chemical	523:530	arg1	composition					532:542	chemical composition	523:542	chemical composition	523:542	The chemical composition and structure of different molecular HmG were analyzed.
29102790	2	47	theme	solution	433:440	arg1	concentration					442:454	the reduced solution concentration	421:454	the reduced solution concentration	421:454	The results indicated that the degradation efficiency increased with the reduced solution concentration, increased H2O2 concentration and ascorbic acid concentration.
29102790	7	48	theme	molecular	1075:1083	arg1	weight					1085:1090	molecular weight	1075:1090	molecular weight	1075:1090	Besides, molecular weight did not obviously affect the antioxidant activities of HmG.
29102790	1	49	theme	degradation	158:168	arg1	method					135:140	An effective method	122:140	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method,	122:262	An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG).
29102790	0	50	theme	glycosaminoglycans	68:85	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and antioxidant properties of low molecular holothurian glycosaminoglycans by H2O2/ascorbic acid degradation.
29102790	0	50	theme	glycosaminoglycans	68:85	arg1	properties					28:37	antioxidant properties	16:37	antioxidant properties	16:37	Preparation and antioxidant properties of low molecular holothurian glycosaminoglycans by H2O2/ascorbic acid degradation.
29102790	0	51	theme	antioxidant	16:26	arg1	properties					28:37	antioxidant properties	16:37	antioxidant properties	16:37	Preparation and antioxidant properties of low molecular holothurian glycosaminoglycans by H2O2/ascorbic acid degradation.
29102790	2	52	theme	degradation	383:393	arg1	efficiency					395:404	the degradation efficiency	379:404	the degradation efficiency	379:404	The results indicated that the degradation efficiency increased with the reduced solution concentration, increased H2O2 concentration and ascorbic acid concentration.
28125217	0	0	theme	Plant	84:88	arg1	fastidiosa					107:116	the Plant Pathogen Xylella fastidiosa	80:116	the Plant Pathogen Xylella fastidiosa	80:116	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	7	1	theme	evolutionary	1584:1595	arg1	hallmark					1597:1604	a morphological and evolutionary hallmark	1564:1604	a morphological and evolutionary hallmark that differentiates two subgroups of CLS-related strains, which may influence interactions between these bacteria and their plant and/or insect hosts	1564:1754	Finally, a comparative sequence analysis suggests that this mutation is likely to result in a morphological and evolutionary hallmark that differentiates two subgroups of CLS-related strains, which may influence interactions between these bacteria and their plant and/or insect hosts.
28125217	4	2	theme	different	843:851	arg1	annotations					853:863	different annotations	843:863	different annotations of X. fastidiosa genomes	843:888	However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions.
28125217	4	3	theme	genomes	882:888	arg1	annotations					853:863	different annotations	843:863	different annotations of X. fastidiosa genomes	843:888	However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions.
28125217	6	4	theme	LPS	1417:1419	arg1	structure					1421:1429	the LPS structure	1413:1429	the LPS structure among CLS-related X. fastidiosa strains	1413:1469	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	4	5	dep	X.	868:869	arg1	fastidiosa					871:880	X. fastidiosa	868:880	X. fastidiosa genomes	868:888	However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions.
28125217	4	6	theme	exact	781:785	arg1	matter					817:822	a matter	815:822	a matter of controversy	815:837	However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions.
28125217	4	6	theme	exact	781:785	arg1	nature					787:792	the exact nature	777:792	the exact nature of this SDR	777:804	However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions.
28125217	5	7	theme	decarboxylases	1071:1084	arg1	subgroup					1055:1062	a subgroup	1053:1062	a subgroup	1053:1062	To confirm the nature of this mutated SDR, a comparative analysis was initially performed, suggesting that it belongs to a subgroup of SDR decarboxylases, representing a UDP-xylose synthase (Uxs).
28125217	6	8	theme	carbohydrate	1230:1241	arg1	analyses					1255:1262	carbohydrate composition analyses	1230:1262	carbohydrate composition analyses	1230:1262	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	0	9	theme	Xylella	99:105	arg1	fastidiosa					107:116	the Plant Pathogen Xylella fastidiosa	80:116	the Plant Pathogen Xylella fastidiosa	80:116	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	1	10	theme	coffee	346:351	arg1	scorch					358:363	coffee leaf scorch	346:363	coffee leaf scorch (CLS)	346:369	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	10	theme	coffee	346:351	arg1	CLS					366:368	CLS	366:368	CLS	366:368	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	2	11	dep	X.	461:462	arg1	subsp					475:479	X. fastidiosa subsp	461:479	X. fastidiosa subsp	461:479	Recent genomic comparisons involving two CLS-related strains, belonging to X. fastidiosa subsp.
28125217	5	12	theme	SDR	970:972	arg1	nature					947:952	the nature	943:952	the nature of this mutated SDR	943:972	To confirm the nature of this mutated SDR, a comparative analysis was initially performed, suggesting that it belongs to a subgroup of SDR decarboxylases, representing a UDP-xylose synthase (Uxs).
28125217	6	13	dep	X.	1449:1450	arg1	fastidiosa					1452:1461	X. fastidiosa	1449:1461	CLS-related X. fastidiosa strains	1437:1469	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	0	14	theme	Pathogen	90:97	arg1	fastidiosa					107:116	the Plant Pathogen Xylella fastidiosa	80:116	the Plant Pathogen Xylella fastidiosa	80:116	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	1	15	theme	leaf	353:356	arg1	scorch					358:363	coffee leaf scorch	346:363	coffee leaf scorch (CLS)	346:369	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	15	theme	leaf	353:356	arg1	CLS					366:368	CLS	366:368	CLS	366:368	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	6	16	theme	recombinant	1156:1166	arg1	derivative					1168:1177	a recombinant derivative	1154:1177	a recombinant derivative of this enzyme	1154:1192	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	6	17	theme	Functional	1129:1138	arg1	assays					1140:1145	Functional assays	1129:1145	Functional assays	1129:1145	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	1	18	theme	Xylella	178:184	arg1	bacillus					218:225	a plant-infecting bacillus	200:225	a plant-infecting bacillus	200:225	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	18	theme	Xylella	178:184	arg1	fastidiosa					186:195	Xylella fastidiosa	178:195	Xylella fastidiosa	178:195	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	5	19	theme	SDR	1067:1069	arg1	decarboxylases					1071:1084	SDR decarboxylases	1067:1084	SDR decarboxylases	1067:1084	To confirm the nature of this mutated SDR, a comparative analysis was initially performed, suggesting that it belongs to a subgroup of SDR decarboxylases, representing a UDP-xylose synthase (Uxs).
28125217	0	20	theme	Functional	0:9	arg1	Characterization					28:43	Functional and Evolutionary Characterization	0:43	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.	0:176	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	7	21	theme	strains	1655:1661	arg1	subgroups					1630:1638	two subgroups	1626:1638	two subgroups	1626:1638	Finally, a comparative sequence analysis suggests that this mutation is likely to result in a morphological and evolutionary hallmark that differentiates two subgroups of CLS-related strains, which may influence interactions between these bacteria and their plant and/or insect hosts.
28125217	6	22	theme	gene	1400:1403	arg1	inactivation					1362:1373	inactivation	1362:1373	inactivation of the X. fastidiosa uxs gene	1362:1403	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	3	23	theme	structural	704:713	arg1	variations					715:724	structural variations	704:724	structural variations in bacterial glycans and glycoconjugates	704:765	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	6	24	theme	composition	1243:1253	arg1	analyses					1255:1262	carbohydrate composition analyses	1230:1262	carbohydrate composition analyses	1230:1262	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	6	25	dep	X.	1382:1383	arg1	fastidiosa					1385:1394	X. fastidiosa	1382:1394	the X. fastidiosa uxs gene	1378:1403	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	3	26	theme	important	673:681	arg1	roles					683:687	important roles	673:687	important roles	673:687	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	2	27	theme	CLS-related	427:437	arg1	strains					439:445	two CLS-related strains	423:445	two CLS-related strains	423:445	Recent genomic comparisons involving two CLS-related strains, belonging to X. fastidiosa subsp.
28125217	4	28	theme	controversy	827:837	arg1	matter					817:822	a matter	815:822	a matter of controversy	815:837	However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions.
28125217	4	28	theme	controversy	827:837	arg1	nature					787:792	the exact nature	777:792	the exact nature of this SDR	777:804	However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions.
28125217	3	29	from	variations	715:724	arg1	glycoconjugates					751:765	glycoconjugates	751:765	glycoconjugates	751:765	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	3	29	from	variations	715:724	arg1	glycans					739:745	bacterial glycans	729:745	bacterial glycans	729:745	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	7	30	theme	insect	1743:1748	arg1	hosts					1750:1754	their plant and/or insect hosts	1724:1754	hosts	1750:1754	Finally, a comparative sequence analysis suggests that this mutation is likely to result in a morphological and evolutionary hallmark that differentiates two subgroups of CLS-related strains, which may influence interactions between these bacteria and their plant and/or insect hosts.
28125217	3	31	theme	frameshift	525:534	arg1	mutation					536:543	a frameshift mutation	523:543	a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates	523:765	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	7	32	theme	CLS-related	1643:1653	arg1	strains					1655:1661	CLS-related strains	1643:1661	CLS-related strains	1643:1661	Finally, a comparative sequence analysis suggests that this mutation is likely to result in a morphological and evolutionary hallmark that differentiates two subgroups of CLS-related strains, which may influence interactions between these bacteria and their plant and/or insect hosts.
28125217	0	33	theme	Evolutionary	15:26	arg1	Characterization					28:43	Functional and Evolutionary Characterization	0:43	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.	0:176	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	3	34	theme	bacterial	729:737	arg1	glycans					739:745	bacterial glycans	729:745	bacterial glycans	729:745	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	7	35	theme	sequence	1495:1502	arg1	analysis					1504:1511	a comparative sequence analysis	1481:1511	a comparative sequence analysis	1481:1511	Finally, a comparative sequence analysis suggests that this mutation is likely to result in a morphological and evolutionary hallmark that differentiates two subgroups of CLS-related strains, which may influence interactions between these bacteria and their plant and/or insect hosts.
28125217	2	36	theme	genomic	393:399	arg1	comparisons					401:411	Recent genomic comparisons	386:411	Recent genomic comparisons	386:411	Recent genomic comparisons involving two CLS-related strains, belonging to X. fastidiosa subsp.
28125217	4	37	theme	distinct	912:919	arg1	reactions					921:929	distinct reactions	912:929	distinct reactions	912:929	However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions.
28125217	3	38	theme	them	510:513	arg1	them					510:513	them	510:513	them	510:513	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	3	38	theme	them	510:513	arg1	one					503:505	one	503:505	one	503:505	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	5	39	theme	comparative	977:987	arg1	analysis					989:996	a comparative analysis	975:996	a comparative analysis	975:996	To confirm the nature of this mutated SDR, a comparative analysis was initially performed, suggesting that it belongs to a subgroup of SDR decarboxylases, representing a UDP-xylose synthase (Uxs).
28125217	2	40	theme	Recent	386:391	arg1	comparisons					401:411	Recent genomic comparisons	386:411	Recent genomic comparisons	386:411	Recent genomic comparisons involving two CLS-related strains, belonging to X. fastidiosa subsp.
28125217	1	41	theme	vineyards	302:310	arg1	disease					291:297	Pierce's disease	282:297	Pierce's disease of vineyards	282:310	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	41	theme	vineyards	302:310	arg1	scorch					358:363	coffee leaf scorch	346:363	coffee leaf scorch (CLS)	346:369	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	41	theme	vineyards	302:310	arg1	chlorosis					331:339	citrus variegated chlorosis	313:339	citrus variegated chlorosis	313:339	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	41	theme	vineyards	302:310	arg1	CLS					366:368	CLS	366:368	CLS	366:368	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	3	42	theme	dehydrogenase/reductase	615:637	arg1	superfamily					645:655	the short-chain dehydrogenase/reductase (SDR) superfamily	599:655	the short-chain dehydrogenase/reductase (SDR) superfamily	599:655	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	4	43	theme	SDR	802:804	arg1	matter					817:822	a matter	815:822	a matter of controversy	815:837	However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions.
28125217	4	43	theme	SDR	802:804	arg1	nature					787:792	the exact nature	777:792	the exact nature of this SDR	777:804	However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions.
28125217	3	44	contain	carries	515:521	arg2	mutation					536:543	a frameshift mutation	523:543	a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates	523:765	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	3	44	contain	carries	515:521	arg1	them					510:513	them	510:513	them	510:513	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	3	44	contain	carries	515:521	arg1	one					503:505	one	503:505	one	503:505	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	0	45	theme	Synthase	61:68	arg1	Gene					70:73	a UDP-Xylose Synthase Gene	48:73	a UDP-Xylose Synthase Gene	48:73	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	7	46	theme	comparative	1483:1493	arg1	analysis					1504:1511	a comparative sequence analysis	1481:1511	a comparative sequence analysis	1481:1511	Finally, a comparative sequence analysis suggests that this mutation is likely to result in a morphological and evolutionary hallmark that differentiates two subgroups of CLS-related strains, which may influence interactions between these bacteria and their plant and/or insect hosts.
28125217	0	47	theme	Bacterial	148:156	arg1	Lipopolysaccharide					158:175	Bacterial Lipopolysaccharide	148:175	Bacterial Lipopolysaccharide	148:175	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	6	48	theme	lipopolysaccharide	1280:1297	arg1	molecules					1305:1313	lipopolysaccharide (LPS) molecules	1280:1313	lipopolysaccharide (LPS) molecules obtained from different strains	1280:1345	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	6	49	theme	uxs	1396:1398	arg1	gene					1400:1403	the X. fastidiosa uxs gene	1378:1403	the X. fastidiosa uxs gene	1378:1403	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	0	50	theme	UDP-Xylose	50:59	arg1	Gene					70:73	a UDP-Xylose Synthase Gene	48:73	a UDP-Xylose Synthase Gene	48:73	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	1	51	theme	citrus	313:318	arg1	chlorosis					331:339	citrus variegated chlorosis	313:339	citrus variegated chlorosis	313:339	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	4	52	theme	X.	868:869	arg1	genomes					882:888	X. fastidiosa genomes	868:888	X. fastidiosa genomes	868:888	However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions.
28125217	1	53	theme	variegated	320:329	arg1	chlorosis					331:339	citrus variegated chlorosis	313:339	citrus variegated chlorosis	313:339	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	0	54	from	fastidiosa	107:116	arg1	Gene					70:73	a UDP-Xylose Synthase Gene	48:73	a UDP-Xylose Synthase Gene	48:73	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	0	54	from	fastidiosa	107:116	arg1	Characterization					28:43	Functional and Evolutionary Characterization	0:43	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.	0:176	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	5	55	theme	mutated	962:968	arg1	SDR					970:972	this mutated SDR	957:972	this mutated SDR	957:972	To confirm the nature of this mutated SDR, a comparative analysis was initially performed, suggesting that it belongs to a subgroup of SDR decarboxylases, representing a UDP-xylose synthase (Uxs).
28125217	6	56	theme	enzyme	1187:1192	arg1	derivative					1168:1177	a recombinant derivative	1154:1177	a recombinant derivative of this enzyme	1154:1192	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	6	57	theme	X.	1449:1450	arg1	strains					1463:1469	CLS-related X. fastidiosa strains	1437:1469	CLS-related X. fastidiosa strains	1437:1469	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	0	58	theme	Lipopolysaccharide	158:175	arg1	Synthesis					135:143	the Synthesis	131:143	the Synthesis of Bacterial Lipopolysaccharide	131:175	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	3	59	theme	superfamily	645:655	arg1	oxidoreductase					581:594	an oxidoreductase	578:594	an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily	578:655	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	3	60	theme	short-chain	603:613	arg1	SDR					640:642	SDR	640:642	SDR	640:642	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	3	60	theme	short-chain	603:613	arg1	dehydrogenase/reductase					615:637	short-chain dehydrogenase/reductase	603:637	the short-chain dehydrogenase/reductase (SDR) superfamily	599:655	pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates.
28125217	6	61	theme	CLS-related	1437:1447	arg1	strains					1463:1469	CLS-related X. fastidiosa strains	1437:1469	CLS-related X. fastidiosa strains	1437:1469	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	1	62	theme	many	244:247	arg1	disease					291:297	Pierce's disease	282:297	Pierce's disease of vineyards	282:310	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	62	theme	many	244:247	arg1	scorch					358:363	coffee leaf scorch	346:363	coffee leaf scorch (CLS)	346:369	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	62	theme	many	244:247	arg1	chlorosis					331:339	citrus variegated chlorosis	313:339	citrus variegated chlorosis	313:339	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	62	theme	many	244:247	arg1	diseases					264:271	many important crop diseases	244:271	many important crop diseases	244:271	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	5	63	theme	UDP-xylose	1102:1111	arg1	Uxs					1123:1125	Uxs	1123:1125	Uxs	1123:1125	To confirm the nature of this mutated SDR, a comparative analysis was initially performed, suggesting that it belongs to a subgroup of SDR decarboxylases, representing a UDP-xylose synthase (Uxs).
28125217	5	63	theme	UDP-xylose	1102:1111	arg1	synthase					1113:1120	a UDP-xylose synthase	1100:1120	a UDP-xylose synthase (Uxs)	1100:1126	To confirm the nature of this mutated SDR, a comparative analysis was initially performed, suggesting that it belongs to a subgroup of SDR decarboxylases, representing a UDP-xylose synthase (Uxs).
28125217	1	64	theme	important	249:257	arg1	disease					291:297	Pierce's disease	282:297	Pierce's disease of vineyards	282:310	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	64	theme	important	249:257	arg1	scorch					358:363	coffee leaf scorch	346:363	coffee leaf scorch (CLS)	346:369	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	64	theme	important	249:257	arg1	chlorosis					331:339	citrus variegated chlorosis	313:339	citrus variegated chlorosis	313:339	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	64	theme	important	249:257	arg1	diseases					264:271	many important crop diseases	244:271	many important crop diseases	244:271	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	0	65	theme	Gene	70:73	arg1	Characterization					28:43	Functional and Evolutionary Characterization	0:43	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.	0:176	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	0	66	from	Characterization	28:43	arg1	fastidiosa					107:116	the Plant Pathogen Xylella fastidiosa	80:116	the Plant Pathogen Xylella fastidiosa	80:116	Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.
28125217	6	67	theme	X.	1382:1383	arg1	gene					1400:1403	the X. fastidiosa uxs gene	1378:1403	the X. fastidiosa uxs gene	1378:1403	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	6	68	theme	different	1329:1337	arg1	strains					1339:1345	different strains	1329:1345	different strains	1329:1345	Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains.
28125217	7	69	theme	morphological	1566:1578	arg1	hallmark					1597:1604	a morphological and evolutionary hallmark	1564:1604	a morphological and evolutionary hallmark that differentiates two subgroups of CLS-related strains, which may influence interactions between these bacteria and their plant and/or insect hosts	1564:1754	Finally, a comparative sequence analysis suggests that this mutation is likely to result in a morphological and evolutionary hallmark that differentiates two subgroups of CLS-related strains, which may influence interactions between these bacteria and their plant and/or insect hosts.
28125217	1	70	theme	crop	259:262	arg1	disease					291:297	Pierce's disease	282:297	Pierce's disease of vineyards	282:310	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	70	theme	crop	259:262	arg1	scorch					358:363	coffee leaf scorch	346:363	coffee leaf scorch (CLS)	346:369	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	70	theme	crop	259:262	arg1	chlorosis					331:339	citrus variegated chlorosis	313:339	citrus variegated chlorosis	313:339	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
28125217	1	70	theme	crop	259:262	arg1	diseases					264:271	many important crop diseases	244:271	many important crop diseases	244:271	Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others.
26423601	3	0	from	abundance	411:419	arg1	leiomyoma					566:574	leiomyoma	566:574	leiomyoma	566:574	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	3	0	from	abundance	411:419	arg1	myometrium					587:596	normal myometrium	580:596	normal myometrium	580:596	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	1	1	theme	common	148:153	arg1	leiomyoma					132:140	Uterine leiomyoma	124:140	Uterine leiomyoma	124:140	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	1	1	theme	common	148:153	arg1	tumors					169:174	a common benign pelvic tumors	146:174	a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM)	146:264	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	6	2	theme	leiomyoma	955:963	arg1	tissue					965:970	leiomyoma tissue	955:970	leiomyoma tissue	955:970	In leiomyoma tissue, abundance of decorin messenger RNA (mRNA) and protein were 2.6-fold and 1.4-fold lower, respectively, compared with normal myometrium.
26423601	0	3	theme	Hormone	69:75	arg1	Proteoglycans					0:12	Proteoglycans	0:12	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.	0:122	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.
26423601	0	3	theme	Hormone	69:75	arg1	Control					77:83	Steroid Hormone Control	61:83	Steroid Hormone Control	61:83	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.
26423601	11	4	theme	growth	1607:1612	arg1	factors					1614:1620	specific growth factors	1598:1620	specific growth factors	1598:1620	As decorin inhibits the activity of specific growth factors, its reduced level in the leiomyoma cell microenvironment may promote cell proliferation and ECM deposition.
26423601	5	5	dep	In	791:792	arg1	vitro					794:798	vitro	794:798	vitro	794:798	In vitro studies were performed on immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells with and without exposure to estradiol and progesterone.
26423601	1	6	theme	benign	155:160	arg1	leiomyoma					132:140	Uterine leiomyoma	124:140	Uterine leiomyoma	124:140	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	1	6	theme	benign	155:160	arg1	tumors					169:174	a common benign pelvic tumors	146:174	a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM)	146:264	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	1	7	theme	extracellular	239:251	arg1	ECM					261:263	ECM	261:263	ECM	261:263	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	1	7	theme	extracellular	239:251	arg1	matrix					253:258	extracellular matrix	239:258	extracellular matrix (ECM)	239:264	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	12	8	theme	decorin	1753:1759	arg1	expression					1761:1770	decorin expression	1753:1770	decorin expression in leiomyoma	1753:1783	Our data suggest that decorin expression in leiomyoma is inhibited by progesterone, which may be a mechanism by which the ovarian steroids affect leiomyoma growth and disease progression.
26423601	0	9	from	Proteoglycans	0:12	arg1	Leiomyoma					17:25	Leiomyoma	17:25	Leiomyoma	17:25	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.
26423601	0	9	from	Proteoglycans	0:12	arg1	Myometrium					38:47	Normal Myometrium	31:47	Normal Myometrium	31:47	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.
26423601	10	10	from	expression	1463:1472	arg1	leiomyoma					1477:1485	leiomyoma	1477:1485	leiomyoma compared with myometrium	1477:1510	Lower decorin expression in leiomyoma compared with myometrium may contribute to disease growth and progression.
26423601	7	11	dep	increased	1207:1215	arg1	compared					1239:1246	compared	1239:1246	compared with myometrium	1239:1262	Abundance of versican mRNA was not different between matched samples, whereas versican protein was increased 1.8-fold in leiomyoma compared with myometrium.
26423601	1	12	theme	matrix	253:258	arg1	ECM					261:263	ECM	261:263	ECM	261:263	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	1	12	theme	matrix	253:258	arg1	amount					229:234	a large amount	221:234	a large amount of extracellular matrix (ECM)	221:264	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	1	12	theme	matrix	253:258	arg1	matrix					253:258	extracellular matrix	239:258	extracellular matrix (ECM)	239:264	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	1	12	theme	matrix	253:258	arg1	cells					211:215	modified smooth muscle cells	188:215	modified smooth muscle cells	188:215	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	12	13	theme	disease	1898:1904	arg1	progression					1906:1916	disease progression	1898:1916	disease progression	1898:1916	Our data suggest that decorin expression in leiomyoma is inhibited by progesterone, which may be a mechanism by which the ovarian steroids affect leiomyoma growth and disease progression.
26423601	7	14	theme	versican	1186:1193	arg1	protein					1195:1201	versican protein	1186:1201	versican protein	1186:1201	Abundance of versican mRNA was not different between matched samples, whereas versican protein was increased 1.8-fold in leiomyoma compared with myometrium.
26423601	3	15	theme	quantitative	460:471	arg1	reaction					500:507	quantitative real-time polymerase chain reaction	460:507	quantitative real-time polymerase chain reaction	460:507	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	9	16	theme	mRNA	1431:1434	arg1	abundance					1410:1418	the abundance	1406:1418	the abundance of decorin mRNA	1406:1434	In UtLM cells, progesterone decreased the abundance of decorin mRNA by 1.3-fold.
26423601	7	17	theme	mRNA	1130:1133	arg1	Abundance					1108:1116	Abundance	1108:1116	Abundance of versican mRNA	1108:1133	Abundance of versican mRNA was not different between matched samples, whereas versican protein was increased 1.8-fold in leiomyoma compared with myometrium.
26423601	6	18	theme	RNA	1004:1006	arg1	abundance					973:981	abundance	973:981	abundance of decorin messenger RNA (mRNA) and protein	973:1025	In leiomyoma tissue, abundance of decorin messenger RNA (mRNA) and protein were 2.6-fold and 1.4-fold lower, respectively, compared with normal myometrium.
26423601	4	19	theme	=	746:746	arg1	n					744:744	n = 17	744:749	n = 17	744:749	Leiomyoma and normal myometrium were collected from women (n = 17) undergoing hysterectomy or myomectomy.
26423601	4	19	theme	=	746:746	arg1	women					737:741	women	737:741	women (n = 17) undergoing hysterectomy or myomectomy	737:788	Leiomyoma and normal myometrium were collected from women (n = 17) undergoing hysterectomy or myomectomy.
26423601	5	20	theme	leiomyoma	839:847	arg1	UtLM					850:853	immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells	826:893	UtLM	850:853	In vitro studies were performed on immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells with and without exposure to estradiol and progesterone.
26423601	1	21	theme	pelvic	162:167	arg1	leiomyoma					132:140	Uterine leiomyoma	124:140	Uterine leiomyoma	124:140	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	1	21	theme	pelvic	162:167	arg1	tumors					169:174	a common benign pelvic tumors	146:174	a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM)	146:264	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	9	22	theme	decorin	1423:1429	arg1	mRNA					1431:1434	decorin mRNA	1423:1434	decorin mRNA	1423:1434	In UtLM cells, progesterone decreased the abundance of decorin mRNA by 1.3-fold.
26423601	5	23	theme	normal	860:865	arg1	cells					889:893	immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells	826:893	cells	889:893	In vitro studies were performed on immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells with and without exposure to estradiol and progesterone.
26423601	9	24	theme	UtLM	1371:1374	arg1	cells					1376:1380	UtLM cells	1371:1380	UtLM cells	1371:1380	In UtLM cells, progesterone decreased the abundance of decorin mRNA by 1.3-fold.
26423601	8	25	theme	secretory	1300:1308	arg1	leiomyoma					1316:1324	secretory phase leiomyoma	1300:1324	secretory phase leiomyoma	1300:1324	Decorin mRNA was 2.4-fold lower in secretory phase leiomyoma compared with proliferative phase tissue.
26423601	5	26	theme	immortalized	826:837	arg1	UtLM					850:853	immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells	826:893	UtLM	850:853	In vitro studies were performed on immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells with and without exposure to estradiol and progesterone.
26423601	7	27	theme	matched	1161:1167	arg1	samples					1169:1175	matched samples	1161:1175	matched samples	1161:1175	Abundance of versican mRNA was not different between matched samples, whereas versican protein was increased 1.8-fold in leiomyoma compared with myometrium.
26423601	6	28	theme	protein	1019:1025	arg1	abundance					973:981	abundance	973:981	abundance of decorin messenger RNA (mRNA) and protein	973:1025	In leiomyoma tissue, abundance of decorin messenger RNA (mRNA) and protein were 2.6-fold and 1.4-fold lower, respectively, compared with normal myometrium.
26423601	3	29	from	expression	445:454	arg1	leiomyoma					566:574	leiomyoma	566:574	leiomyoma	566:574	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	3	29	from	expression	445:454	arg1	myometrium					587:596	normal myometrium	580:596	normal myometrium	580:596	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	11	30	from	level	1635:1639	arg1	microenvironment					1663:1678	the leiomyoma cell microenvironment	1644:1678	the leiomyoma cell microenvironment	1644:1678	As decorin inhibits the activity of specific growth factors, its reduced level in the leiomyoma cell microenvironment may promote cell proliferation and ECM deposition.
26423601	2	31	theme	disease	361:367	arg1	pathophysiology					338:352	pathophysiology	338:352	pathophysiology of the disease	338:367	The proteoglycan composition of the leiomyoma ECM is thought to affect pathophysiology of the disease.
26423601	7	32	theme	versican	1121:1128	arg1	mRNA					1130:1133	versican mRNA	1121:1133	versican mRNA	1121:1133	Abundance of versican mRNA was not different between matched samples, whereas versican protein was increased 1.8-fold in leiomyoma compared with myometrium.
26423601	6	33	theme	normal	1089:1094	arg1	myometrium					1096:1105	normal myometrium	1089:1105	normal myometrium	1089:1105	In leiomyoma tissue, abundance of decorin messenger RNA (mRNA) and protein were 2.6-fold and 1.4-fold lower, respectively, compared with normal myometrium.
26423601	6	34	from	tissue	965:970	arg1	2.6-fold					1032:1039	2.6-fold	1032:1039	2.6-fold	1032:1039	In leiomyoma tissue, abundance of decorin messenger RNA (mRNA) and protein were 2.6-fold and 1.4-fold lower, respectively, compared with normal myometrium.
26423601	12	35	theme	leiomyoma	1877:1885	arg1	growth					1887:1892	leiomyoma growth	1877:1892	leiomyoma growth	1877:1892	Our data suggest that decorin expression in leiomyoma is inhibited by progesterone, which may be a mechanism by which the ovarian steroids affect leiomyoma growth and disease progression.
26423601	3	36	theme	proteoglycans	517:529	arg1	abundance					411:419	the abundance	407:419	the abundance (by immunoblotting)	407:439	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	3	36	theme	proteoglycans	517:529	arg1	expression					445:454	expression	445:454	expression (by quantitative real-time polymerase chain reaction)	445:508	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	1	37	theme	modified	188:195	arg1	cells					211:215	modified smooth muscle cells	188:215	modified smooth muscle cells	188:215	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	5	38	theme	In	791:792	arg1	studies					800:806	In vitro studies	791:806	In vitro studies	791:806	In vitro studies were performed on immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells with and without exposure to estradiol and progesterone.
26423601	8	39	theme	phase	1310:1314	arg1	leiomyoma					1316:1324	secretory phase leiomyoma	1300:1324	secretory phase leiomyoma	1300:1324	Decorin mRNA was 2.4-fold lower in secretory phase leiomyoma compared with proliferative phase tissue.
26423601	2	40	theme	proteoglycan	271:282	arg1	composition					284:294	The proteoglycan composition	267:294	The proteoglycan composition of the leiomyoma ECM	267:315	The proteoglycan composition of the leiomyoma ECM is thought to affect pathophysiology of the disease.
26423601	1	41	theme	smooth	197:202	arg1	cells					211:215	modified smooth muscle cells	188:215	modified smooth muscle cells	188:215	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	11	42	theme	reduced	1627:1633	arg1	level					1635:1639	its reduced level	1623:1639	its reduced level in the leiomyoma cell microenvironment	1623:1678	As decorin inhibits the activity of specific growth factors, its reduced level in the leiomyoma cell microenvironment may promote cell proliferation and ECM deposition.
26423601	10	43	theme	Lower	1449:1453	arg1	expression					1463:1472	Lower decorin expression	1449:1472	Lower decorin expression in leiomyoma compared with myometrium	1449:1510	Lower decorin expression in leiomyoma compared with myometrium may contribute to disease growth and progression.
26423601	6	44	theme	messenger	994:1002	arg1	RNA					1004:1006	decorin messenger RNA	986:1006	decorin messenger RNA (mRNA)	986:1013	In leiomyoma tissue, abundance of decorin messenger RNA (mRNA) and protein were 2.6-fold and 1.4-fold lower, respectively, compared with normal myometrium.
26423601	6	44	theme	messenger	994:1002	arg1	mRNA					1009:1012	mRNA	1009:1012	mRNA	1009:1012	In leiomyoma tissue, abundance of decorin messenger RNA (mRNA) and protein were 2.6-fold and 1.4-fold lower, respectively, compared with normal myometrium.
26423601	10	45	dep	disease	1530:1536	arg1	growth					1538:1543	growth	1538:1543	growth	1538:1543	Lower decorin expression in leiomyoma compared with myometrium may contribute to disease growth and progression.
26423601	10	45	dep	disease	1530:1536	arg1	progression					1549:1559	progression	1549:1559	progression	1549:1559	Lower decorin expression in leiomyoma compared with myometrium may contribute to disease growth and progression.
26423601	1	46	theme	muscle	204:209	arg1	cells					211:215	modified smooth muscle cells	188:215	modified smooth muscle cells	188:215	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	0	47	theme	Normal	31:36	arg1	Myometrium					38:47	Normal Myometrium	31:47	Normal Myometrium	31:47	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.
26423601	8	48	theme	Decorin	1265:1271	arg1	mRNA					1273:1276	Decorin mRNA	1265:1276	Decorin mRNA	1265:1276	Decorin mRNA was 2.4-fold lower in secretory phase leiomyoma compared with proliferative phase tissue.
26423601	8	49	theme	phase	1354:1358	arg1	tissue					1360:1365	proliferative phase tissue	1340:1365	proliferative phase tissue	1340:1365	Decorin mRNA was 2.4-fold lower in secretory phase leiomyoma compared with proliferative phase tissue.
26423601	6	50	theme	decorin	986:992	arg1	RNA					1004:1006	decorin messenger RNA	986:1006	decorin messenger RNA (mRNA)	986:1013	In leiomyoma tissue, abundance of decorin messenger RNA (mRNA) and protein were 2.6-fold and 1.4-fold lower, respectively, compared with normal myometrium.
26423601	6	50	theme	decorin	986:992	arg1	mRNA					1009:1012	mRNA	1009:1012	mRNA	1009:1012	In leiomyoma tissue, abundance of decorin messenger RNA (mRNA) and protein were 2.6-fold and 1.4-fold lower, respectively, compared with normal myometrium.
26423601	11	51	theme	cell	1658:1661	arg1	microenvironment					1663:1678	the leiomyoma cell microenvironment	1644:1678	the leiomyoma cell microenvironment	1644:1678	As decorin inhibits the activity of specific growth factors, its reduced level in the leiomyoma cell microenvironment may promote cell proliferation and ECM deposition.
26423601	12	52	from	expression	1761:1770	arg1	leiomyoma					1775:1783	leiomyoma	1775:1783	leiomyoma	1775:1783	Our data suggest that decorin expression in leiomyoma is inhibited by progesterone, which may be a mechanism by which the ovarian steroids affect leiomyoma growth and disease progression.
26423601	11	53	theme	ECM	1715:1717	arg1	deposition					1719:1728	ECM deposition	1715:1728	ECM deposition	1715:1728	As decorin inhibits the activity of specific growth factors, its reduced level in the leiomyoma cell microenvironment may promote cell proliferation and ECM deposition.
26423601	5	54	theme	myometrial	867:876	arg1	cells					889:893	immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells	826:893	cells	889:893	In vitro studies were performed on immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells with and without exposure to estradiol and progesterone.
26423601	11	55	theme	factors	1614:1620	arg1	activity					1586:1593	the activity	1582:1593	the activity of specific growth factors	1582:1620	As decorin inhibits the activity of specific growth factors, its reduced level in the leiomyoma cell microenvironment may promote cell proliferation and ECM deposition.
26423601	10	56	theme	decorin	1455:1461	arg1	expression					1463:1472	Lower decorin expression	1449:1472	Lower decorin expression in leiomyoma compared with myometrium	1449:1510	Lower decorin expression in leiomyoma compared with myometrium may contribute to disease growth and progression.
26423601	12	57	theme	ovarian	1853:1859	arg1	steroids					1861:1868	the ovarian steroids	1849:1868	the ovarian steroids	1849:1868	Our data suggest that decorin expression in leiomyoma is inhibited by progesterone, which may be a mechanism by which the ovarian steroids affect leiomyoma growth and disease progression.
26423601	3	58	theme	real-time	473:481	arg1	reaction					500:507	quantitative real-time polymerase chain reaction	460:507	quantitative real-time polymerase chain reaction	460:507	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	4	59	theme	normal	699:704	arg1	myometrium					706:715	normal myometrium	699:715	normal myometrium	699:715	Leiomyoma and normal myometrium were collected from women (n = 17) undergoing hysterectomy or myomectomy.
26423601	11	60	theme	specific	1598:1605	arg1	factors					1614:1620	specific growth factors	1598:1620	specific growth factors	1598:1620	As decorin inhibits the activity of specific growth factors, its reduced level in the leiomyoma cell microenvironment may promote cell proliferation and ECM deposition.
26423601	11	61	theme	leiomyoma	1648:1656	arg1	microenvironment					1663:1678	the leiomyoma cell microenvironment	1644:1678	the leiomyoma cell microenvironment	1644:1678	As decorin inhibits the activity of specific growth factors, its reduced level in the leiomyoma cell microenvironment may promote cell proliferation and ECM deposition.
26423601	3	62	theme	steroid	647:653	arg1	hormones					655:662	steroid hormones	647:662	steroid hormones	647:662	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	3	63	theme	normal	580:585	arg1	myometrium					587:596	normal myometrium	580:596	normal myometrium	580:596	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	1	64	theme	Uterine	124:130	arg1	leiomyoma					132:140	Uterine leiomyoma	124:140	Uterine leiomyoma	124:140	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	1	64	theme	Uterine	124:130	arg1	tumors					169:174	a common benign pelvic tumors	146:174	a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM)	146:264	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	8	65	theme	proliferative	1340:1352	arg1	tissue					1360:1365	proliferative phase tissue	1340:1365	proliferative phase tissue	1340:1365	Decorin mRNA was 2.4-fold lower in secretory phase leiomyoma compared with proliferative phase tissue.
26423601	5	66	theme	hTERT-HM	879:886	arg1	cells					889:893	immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells	826:893	cells	889:893	In vitro studies were performed on immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells with and without exposure to estradiol and progesterone.
26423601	6	67	from	2.6-fold	1032:1039	arg1	tissue					965:970	leiomyoma tissue	955:970	leiomyoma tissue	955:970	In leiomyoma tissue, abundance of decorin messenger RNA (mRNA) and protein were 2.6-fold and 1.4-fold lower, respectively, compared with normal myometrium.
26423601	2	68	theme	leiomyoma	303:311	arg1	ECM					313:315	the leiomyoma ECM	299:315	the leiomyoma ECM	299:315	The proteoglycan composition of the leiomyoma ECM is thought to affect pathophysiology of the disease.
26423601	2	69	theme	ECM	313:315	arg1	composition					284:294	The proteoglycan composition	267:294	The proteoglycan composition of the leiomyoma ECM	267:315	The proteoglycan composition of the leiomyoma ECM is thought to affect pathophysiology of the disease.
26423601	0	70	theme	Steroid	61:67	arg1	Proteoglycans					0:12	Proteoglycans	0:12	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.	0:122	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.
26423601	0	70	theme	Steroid	61:67	arg1	Control					77:83	Steroid Hormone Control	61:83	Steroid Hormone Control	61:83	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.
26423601	11	71	theme	cell	1692:1695	arg1	proliferation					1697:1709	cell proliferation	1692:1709	cell proliferation	1692:1709	As decorin inhibits the activity of specific growth factors, its reduced level in the leiomyoma cell microenvironment may promote cell proliferation and ECM deposition.
26423601	3	72	dep	proteoglycans	517:529	arg1	versican					554:561	versican	554:561	versican	554:561	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	3	72	dep	proteoglycans	517:529	arg1	biglycan					531:538	biglycan	531:538	biglycan	531:538	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	3	72	dep	proteoglycans	517:529	arg1	proteoglycans					517:529	the proteoglycans biglycan, decorin, and versican	513:561	the proteoglycans biglycan, decorin, and versican	513:561	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	3	72	dep	proteoglycans	517:529	arg1	decorin					541:547	decorin	541:547	decorin	541:547	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	3	73	theme	polymerase	483:492	arg1	reaction					500:507	quantitative real-time polymerase chain reaction	460:507	quantitative real-time polymerase chain reaction	460:507	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	1	74	theme	large	223:227	arg1	ECM					261:263	ECM	261:263	ECM	261:263	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	1	74	theme	large	223:227	arg1	amount					229:234	a large amount	221:234	a large amount of extracellular matrix (ECM)	221:264	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	1	74	theme	large	223:227	arg1	matrix					253:258	extracellular matrix	239:258	extracellular matrix (ECM)	239:264	Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM).
26423601	3	75	theme	menstrual	668:676	arg1	phase					678:682	menstrual phase	668:682	menstrual phase	668:682	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26423601	0	76	dep	Proteoglycans	0:12	arg1	Proteoglycans					0:12	Proteoglycans	0:12	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.	0:122	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.
26423601	0	76	dep	Proteoglycans	0:12	arg1	Control					77:83	Steroid Hormone Control	61:83	Steroid Hormone Control	61:83	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.
26423601	0	76	dep	Proteoglycans	0:12	arg1	Implications					90:101	Implications	90:101	Implications for Pathophysiology	90:121	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.
26423601	0	76	dep	Proteoglycans	0:12	arg1	Abundance					50:58	Abundance	50:58	Abundance	50:58	Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.
26423601	3	77	theme	chain	494:498	arg1	reaction					500:507	quantitative real-time polymerase chain reaction	460:507	quantitative real-time polymerase chain reaction	460:507	To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase.
26917403	0	0	theme	pulp	81:84	arg1	component					50:58	the cellulosic component	35:58	the cellulosic component of thermo mechanical pulp	35:84	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.
26917403	2	1	theme	iodomethane	336:346	arg1	use					329:331	the use	325:331	the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials	325:514	This study details the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials.
26917403	0	2	theme	mechanical	70:79	arg1	pulp					81:84	thermo mechanical pulp	63:84	thermo mechanical pulp	63:84	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.
26917403	2	3	theme	composite	496:504	arg1	materials					506:514	composite materials	496:514	composite materials	496:514	This study details the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials.
26917403	4	4	theme	cellulose	735:743	arg1	components					756:765	cellulose and lignin components	735:765	cellulose and lignin components of TMP	735:772	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
26917403	6	5	theme	other	944:948	arg1	hand					950:953	the other hand	940:953	the other hand	940:953	On the other hand, pulp treated at 3 h using high concentrations of caustic were characterized with surfaces that were more hydrophobic.
26917403	7	6	theme	pulp	1178:1181	arg1	samples					1183:1189	pulp samples	1178:1189	pulp samples for composite applications	1178:1216	Thus, this study outlines a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications.
26917403	4	7	with	reaction	677:684	arg1	components					756:765	cellulose and lignin components	735:765	cellulose and lignin components of TMP	735:772	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
26917403	4	7	with	reaction	677:684	arg1	groups					725:730	the hydroxyl groups	712:730	the hydroxyl groups of cellulose and lignin components of TMP	712:772	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
26917403	5	8	theme	low	810:812	arg1	concentration					822:834	low caustic concentration	810:834	low caustic concentration	810:834	Pulp samples treated for 4 h or at low caustic concentration showed the least improvements plausibly due to pulp degradation or poor pulp swelling, respectively.
26917403	7	9	theme	solvent-free	1119:1130	arg1	method					1143:1148	a fast and organic solvent-free (clean up) method	1100:1148	a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications	1100:1216	Thus, this study outlines a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications.
26917403	7	10	theme	fast	1102:1105	arg1	method					1143:1148	a fast and organic solvent-free (clean up) method	1100:1148	a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications	1100:1216	Thus, this study outlines a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications.
26917403	6	11	theme	high	982:985	arg1	concentrations					987:1000	high concentrations	982:1000	high concentrations of caustic	982:1011	On the other hand, pulp treated at 3 h using high concentrations of caustic were characterized with surfaces that were more hydrophobic.
26917403	4	12	theme	lignin	749:754	arg1	components					756:765	cellulose and lignin components	735:765	cellulose and lignin components of TMP	735:772	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
26917403	6	13	theme	caustic	1005:1011	arg1	concentrations					987:1000	high concentrations	982:1000	high concentrations of caustic	982:1011	On the other hand, pulp treated at 3 h using high concentrations of caustic were characterized with surfaces that were more hydrophobic.
26917403	1	14	theme	chemical	163:170	arg1	methods					172:178	chemical methods	163:178	chemical methods	163:178	The feasibility of employing chemical methods for enhancement of cellulose-based materials is dependent on the availability, price, and green index of the modifying agent.
26917403	0	15	theme	rapid	94:98	arg1	method					100:105	a rapid method	92:105	a rapid method	92:105	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.
26917403	7	16	theme	organic	1111:1117	arg1	method					1143:1148	a fast and organic solvent-free (clean up) method	1100:1148	a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications	1100:1216	Thus, this study outlines a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications.
26917403	5	17	theme	caustic	814:820	arg1	concentration					822:834	low caustic concentration	810:834	low caustic concentration	810:834	Pulp samples treated for 4 h or at low caustic concentration showed the least improvements plausibly due to pulp degradation or poor pulp swelling, respectively.
26917403	5	18	theme	Pulp	775:778	arg1	samples					780:786	Pulp samples	775:786	Pulp samples treated for 4 h or at low caustic concentration	775:834	Pulp samples treated for 4 h or at low caustic concentration showed the least improvements plausibly due to pulp degradation or poor pulp swelling, respectively.
26917403	2	19	theme	organo	364:369	arg1	halide					371:376	an inexpensive organo halide	349:376	an inexpensive organo halide	349:376	This study details the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials.
26917403	2	19	theme	organo	364:369	arg1	iodomethane					336:346	iodomethane	336:346	iodomethane	336:346	This study details the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials.
26917403	2	20	theme	mechanical	420:429	arg1	samples					437:443	thermo mechanical (TMP) samples	413:443	thermo mechanical (TMP) samples	413:443	This study details the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials.
26917403	1	21	theme	green	270:274	arg1	index					276:280	green index	270:280	green index	270:280	The feasibility of employing chemical methods for enhancement of cellulose-based materials is dependent on the availability, price, and green index of the modifying agent.
26917403	3	22	theme	time	639:642	arg1	influence					549:557	the influence	545:557	the influence of various concentration of iodomethane, concentration of caustic, and reaction time	545:642	For this system, we studied the influence of various concentration of iodomethane, concentration of caustic, and reaction time.
26917403	2	23	theme	inexpensive	352:362	arg1	halide					371:376	an inexpensive organo halide	349:376	an inexpensive organo halide	349:376	This study details the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials.
26917403	2	23	theme	inexpensive	352:362	arg1	iodomethane					336:346	iodomethane	336:346	iodomethane	336:346	This study details the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials.
26917403	2	24	theme	thermo	413:418	arg1	samples					437:443	thermo mechanical (TMP) samples	413:443	thermo mechanical (TMP) samples	413:443	This study details the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials.
26917403	0	25	theme	Iodomethane	108:118	arg1	modification					120:131	Iodomethane modification	108:131	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.	0:132	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.
26917403	5	26	theme	pulp	908:911	arg1	swelling					913:920	poor pulp swelling	903:920	poor pulp swelling	903:920	Pulp samples treated for 4 h or at low caustic concentration showed the least improvements plausibly due to pulp degradation or poor pulp swelling, respectively.
26917403	0	27	theme	thermal	12:18	arg1	enhancement					20:30	thermal enhancement	12:30	thermal enhancement of the cellulosic component of thermo mechanical pulp	12:84	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.
26917403	5	28	theme	poor	903:906	arg1	swelling					913:920	poor pulp swelling	903:920	poor pulp swelling	903:920	Pulp samples treated for 4 h or at low caustic concentration showed the least improvements plausibly due to pulp degradation or poor pulp swelling, respectively.
26917403	3	29	theme	caustic	617:623	arg1	concentration					600:612	concentration	600:612	concentration of caustic	600:623	For this system, we studied the influence of various concentration of iodomethane, concentration of caustic, and reaction time.
26917403	3	29	theme	caustic	617:623	arg1	time					639:642	reaction time	630:642	reaction time	630:642	For this system, we studied the influence of various concentration of iodomethane, concentration of caustic, and reaction time.
26917403	3	29	theme	caustic	617:623	arg1	concentration					570:582	various concentration	562:582	various concentration of iodomethane	562:597	For this system, we studied the influence of various concentration of iodomethane, concentration of caustic, and reaction time.
26917403	4	30	theme	hydroxyl	716:723	arg1	components					756:765	cellulose and lignin components	735:765	cellulose and lignin components of TMP	735:772	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
26917403	4	30	theme	hydroxyl	716:723	arg1	groups					725:730	the hydroxyl groups	712:730	the hydroxyl groups of cellulose and lignin components of TMP	712:772	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
26917403	1	31	theme	cellulose-based	199:213	arg1	materials					215:223	cellulose-based materials	199:223	cellulose-based materials	199:223	The feasibility of employing chemical methods for enhancement of cellulose-based materials is dependent on the availability, price, and green index of the modifying agent.
26917403	7	32	theme	clean	1133:1137	arg1	solvent-free					1119:1130	solvent-free	1119:1130	a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications	1100:1216	Thus, this study outlines a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications.
26917403	7	32	theme	clean	1133:1137	arg1	up					1139:1140	clean up	1133:1140	clean up	1133:1140	Thus, this study outlines a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications.
26917403	1	33	dep	availability	245:256	arg1	the					241:243	the	241:243	the	241:243	The feasibility of employing chemical methods for enhancement of cellulose-based materials is dependent on the availability, price, and green index of the modifying agent.
26917403	1	34	theme	materials	215:223	arg1	enhancement					184:194	enhancement	184:194	enhancement of cellulose-based materials	184:223	The feasibility of employing chemical methods for enhancement of cellulose-based materials is dependent on the availability, price, and green index of the modifying agent.
26917403	2	35	theme	samples	437:443	arg1	hydrophobicity					395:408	the hydrophobicity	391:408	the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials	391:514	This study details the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials.
26917403	1	36	theme	modifying	289:297	arg1	agent					299:303	the modifying agent	285:303	the modifying agent	285:303	The feasibility of employing chemical methods for enhancement of cellulose-based materials is dependent on the availability, price, and green index of the modifying agent.
26917403	3	37	theme	concentration	600:612	arg1	influence					549:557	the influence	545:557	the influence of various concentration of iodomethane, concentration of caustic, and reaction time	545:642	For this system, we studied the influence of various concentration of iodomethane, concentration of caustic, and reaction time.
26917403	3	38	theme	various	562:568	arg1	concentration					570:582	various concentration	562:582	various concentration of iodomethane	562:597	For this system, we studied the influence of various concentration of iodomethane, concentration of caustic, and reaction time.
26917403	2	39	theme	structural	472:481	arg1	elements					483:490	structural elements	472:490	structural elements for composite materials	472:514	This study details the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials.
26917403	0	40	theme	component	50:58	arg1	Surface					0:6	Surface	0:6	Surface	0:6	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.
26917403	0	40	theme	component	50:58	arg1	enhancement					20:30	thermal enhancement	12:30	thermal enhancement of the cellulosic component of thermo mechanical pulp	12:84	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.
26917403	1	41	theme	agent	299:303	arg1	price					259:263	price	259:263	price	259:263	The feasibility of employing chemical methods for enhancement of cellulose-based materials is dependent on the availability, price, and green index of the modifying agent.
26917403	1	41	theme	agent	299:303	arg1	availability					245:256	availability	245:256	availability	245:256	The feasibility of employing chemical methods for enhancement of cellulose-based materials is dependent on the availability, price, and green index of the modifying agent.
26917403	1	41	theme	agent	299:303	arg1	index					276:280	green index	270:280	green index	270:280	The feasibility of employing chemical methods for enhancement of cellulose-based materials is dependent on the availability, price, and green index of the modifying agent.
26917403	0	42	theme	cellulosic	39:48	arg1	component					50:58	the cellulosic component	35:58	the cellulosic component of thermo mechanical pulp	35:84	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.
26917403	2	43	theme	TMP	432:434	arg1	samples					437:443	thermo mechanical (TMP) samples	413:443	thermo mechanical (TMP) samples	413:443	This study details the use of iodomethane, an inexpensive organo halide, to increase the hydrophobicity of thermo mechanical (TMP) samples, which renders them better structural elements for composite materials.
26917403	7	44	theme	composite	1195:1203	arg1	applications					1205:1216	composite applications	1195:1216	composite applications	1195:1216	Thus, this study outlines a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications.
26917403	4	45	theme	TMP	770:772	arg1	components					756:765	cellulose and lignin components	735:765	cellulose and lignin components of TMP	735:772	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
26917403	7	46	used	used	1162:1165	arg2	method					1143:1148	a fast and organic solvent-free (clean up) method	1100:1148	a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications	1100:1216	Thus, this study outlines a fast and organic solvent-free (clean up) method that can be used to enhance pulp samples for composite applications.
26917403	3	47	theme	iodomethane	587:597	arg1	concentration					600:612	concentration	600:612	concentration of caustic	600:623	For this system, we studied the influence of various concentration of iodomethane, concentration of caustic, and reaction time.
26917403	3	47	theme	iodomethane	587:597	arg1	time					639:642	reaction time	630:642	reaction time	630:642	For this system, we studied the influence of various concentration of iodomethane, concentration of caustic, and reaction time.
26917403	3	47	theme	iodomethane	587:597	arg1	concentration					570:582	various concentration	562:582	various concentration of iodomethane	562:597	For this system, we studied the influence of various concentration of iodomethane, concentration of caustic, and reaction time.
26917403	4	48	theme	halide	700:705	arg1	reaction					677:684	reaction	677:684	reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP	677:772	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
26917403	4	49	theme	components	756:765	arg1	components					756:765	cellulose and lignin components	735:765	cellulose and lignin components of TMP	735:772	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
26917403	4	49	theme	components	756:765	arg1	groups					725:730	the hydroxyl groups	712:730	the hydroxyl groups of cellulose and lignin components of TMP	712:772	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
26917403	4	50	theme	organo	693:698	arg1	halide					700:705	the organo halide	689:705	the organo halide	689:705	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
26917403	5	51	theme	pulp	883:886	arg1	degradation					888:898	pulp degradation	883:898	pulp degradation	883:898	Pulp samples treated for 4 h or at low caustic concentration showed the least improvements plausibly due to pulp degradation or poor pulp swelling, respectively.
26917403	0	52	theme	thermo	63:68	arg1	pulp					81:84	thermo mechanical pulp	63:84	thermo mechanical pulp	63:84	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.
26917403	3	53	theme	reaction	630:637	arg1	time					639:642	reaction time	630:642	reaction time	630:642	For this system, we studied the influence of various concentration of iodomethane, concentration of caustic, and reaction time.
26917403	3	54	theme	concentration	570:582	arg1	influence					549:557	the influence	545:557	the influence of various concentration of iodomethane, concentration of caustic, and reaction time	545:642	For this system, we studied the influence of various concentration of iodomethane, concentration of caustic, and reaction time.
26917403	5	55	theme	least	847:851	arg1	improvements					853:864	the least improvements	843:864	the least improvements	843:864	Pulp samples treated for 4 h or at low caustic concentration showed the least improvements plausibly due to pulp degradation or poor pulp swelling, respectively.
26917403	0	56	dep	Surface	0:6	arg1	modification					120:131	Iodomethane modification	108:131	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.	0:132	Surface and thermal enhancement of the cellulosic component of thermo mechanical pulp using a rapid method: Iodomethane modification.
26917403	4	57	theme	Infrared	645:652	arg1	spectroscopy					654:665	Infrared spectroscopy	645:665	Infrared spectroscopy	645:665	Infrared spectroscopy suggested reaction of the organo halide with the hydroxyl groups of cellulose and lignin components of TMP.
25942815	1	0	theme	optical	190:196	arg1	properties					225:234	their optical, mechanical and electrical properties	184:234	their optical, mechanical and electrical properties	184:234	Cellulose and one dimensional nano-material composite has been investigated for various industrial applications due to their optical, mechanical and electrical properties.
25942815	5	1	theme	host	799:802	arg1	matrix					804:809	the cellulose host matrix	785:809	the cellulose host matrix	785:809	In the sol-gel process, the inorganic particles were dispersed in the cellulose host matrix at the nanometer scale, bonding to the cellulose through the covalent bonds.
25942815	4	2	theme	nano-materials	617:630	arg1	properties					560:569	The chemical and morphological properties	529:569	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids	529:665	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids were confirmed by FTIR, XRD, SEM, and TEM analysis.
25942815	5	3	theme	inorganic	747:755	arg1	particles					757:765	the inorganic particles	743:765	the inorganic particles	743:765	In the sol-gel process, the inorganic particles were dispersed in the cellulose host matrix at the nanometer scale, bonding to the cellulose through the covalent bonds.
25942815	2	4	theme	cross-linking	350:362	arg1	process					364:370	sol-gel covalent cross-linking process	333:370	sol-gel covalent cross-linking process	333:370	In present investigation, cellulose/silica and silica-gold hybrid biomaterials were prepared by sol-gel covalent cross-linking process.
25942815	5	5	theme	nanometer	818:826	arg1	scale					828:832	the nanometer scale	814:832	the nanometer scale	814:832	In the sol-gel process, the inorganic particles were dispersed in the cellulose host matrix at the nanometer scale, bonding to the cellulose through the covalent bonds.
25942815	1	6	theme	dimensional	83:93	arg1	composite					109:117	one dimensional nano-material composite	79:117	one dimensional nano-material composite	79:117	Cellulose and one dimensional nano-material composite has been investigated for various industrial applications due to their optical, mechanical and electrical properties.
25942815	2	7	theme	covalent	341:348	arg1	process					364:370	sol-gel covalent cross-linking process	333:370	sol-gel covalent cross-linking process	333:370	In present investigation, cellulose/silica and silica-gold hybrid biomaterials were prepared by sol-gel covalent cross-linking process.
25942815	3	8	theme	gold	407:410	arg1	precursors					412:421	gold precursors	407:421	gold precursors	407:421	The tetraethoxysiliane (TEOS) and gold precursors and γ-aminopropyltriethoxysilane (γ-APTES) as coupling agent were used for sol-gel cross-linking process.
25942815	4	9	theme	morphological	546:558	arg1	properties					560:569	The chemical and morphological properties	529:569	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids	529:665	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids were confirmed by FTIR, XRD, SEM, and TEM analysis.
25942815	1	10	theme	nano-material	95:107	arg1	composite					109:117	one dimensional nano-material composite	79:117	one dimensional nano-material composite	79:117	Cellulose and one dimensional nano-material composite has been investigated for various industrial applications due to their optical, mechanical and electrical properties.
25942815	1	11	theme	mechanical	199:208	arg1	properties					225:234	their optical, mechanical and electrical properties	184:234	their optical, mechanical and electrical properties	184:234	Cellulose and one dimensional nano-material composite has been investigated for various industrial applications due to their optical, mechanical and electrical properties.
25942815	4	12	theme	cellulose/silica	574:589	arg1	properties					560:569	The chemical and morphological properties	529:569	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids	529:665	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids were confirmed by FTIR, XRD, SEM, and TEM analysis.
25942815	5	13	theme	sol-gel	726:732	arg1	process					734:740	the sol-gel process	722:740	the sol-gel process	722:740	In the sol-gel process, the inorganic particles were dispersed in the cellulose host matrix at the nanometer scale, bonding to the cellulose through the covalent bonds.
25942815	1	14	theme	electrical	214:223	arg1	properties					225:234	their optical, mechanical and electrical properties	184:234	their optical, mechanical and electrical properties	184:234	Cellulose and one dimensional nano-material composite has been investigated for various industrial applications due to their optical, mechanical and electrical properties.
25942815	4	15	dep	cellulose/silica	574:589	arg1	hybrids					659:665	hybrids	659:665	hybrids	659:665	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids were confirmed by FTIR, XRD, SEM, and TEM analysis.
25942815	3	16	theme	sol-gel	498:504	arg1	process					520:526	sol-gel cross-linking process	498:526	sol-gel cross-linking process	498:526	The tetraethoxysiliane (TEOS) and gold precursors and γ-aminopropyltriethoxysilane (γ-APTES) as coupling agent were used for sol-gel cross-linking process.
25942815	0	17	theme	electronic	40:49	arg1	applications					51:62	electronic applications	40:62	electronic applications	40:62	Cellulose-silica/gold nanomaterials for electronic applications.
25942815	5	18	theme	cellulose	789:797	arg1	matrix					804:809	the cellulose host matrix	785:809	the cellulose host matrix	785:809	In the sol-gel process, the inorganic particles were dispersed in the cellulose host matrix at the nanometer scale, bonding to the cellulose through the covalent bonds.
25942815	2	19	theme	sol-gel	333:339	arg1	process					364:370	sol-gel covalent cross-linking process	333:370	sol-gel covalent cross-linking process	333:370	In present investigation, cellulose/silica and silica-gold hybrid biomaterials were prepared by sol-gel covalent cross-linking process.
25942815	2	20	theme	silica-gold	284:294	arg1	biomaterials					303:314	silica-gold hybrid biomaterials	284:314	silica-gold hybrid biomaterials	284:314	In present investigation, cellulose/silica and silica-gold hybrid biomaterials were prepared by sol-gel covalent cross-linking process.
25942815	3	21	theme	cross-linking	506:518	arg1	process					520:526	sol-gel cross-linking process	498:526	sol-gel cross-linking process	498:526	The tetraethoxysiliane (TEOS) and gold precursors and γ-aminopropyltriethoxysilane (γ-APTES) as coupling agent were used for sol-gel cross-linking process.
25942815	3	22	theme	coupling	469:476	arg1	agent					478:482	coupling agent	469:482	coupling agent	469:482	The tetraethoxysiliane (TEOS) and gold precursors and γ-aminopropyltriethoxysilane (γ-APTES) as coupling agent were used for sol-gel cross-linking process.
25942815	4	23	theme	covalent	636:643	arg1	cross-linking					645:657	covalent cross-linking	636:657	covalent cross-linking	636:657	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids were confirmed by FTIR, XRD, SEM, and TEM analysis.
25942815	4	24	theme	chemical	533:540	arg1	properties					560:569	The chemical and morphological properties	529:569	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids	529:665	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids were confirmed by FTIR, XRD, SEM, and TEM analysis.
25942815	1	25	theme	various	145:151	arg1	applications					164:175	various industrial applications	145:175	various industrial applications due to their optical, mechanical and electrical properties	145:234	Cellulose and one dimensional nano-material composite has been investigated for various industrial applications due to their optical, mechanical and electrical properties.
25942815	1	26	theme	industrial	153:162	arg1	applications					164:175	various industrial applications	145:175	various industrial applications due to their optical, mechanical and electrical properties	145:234	Cellulose and one dimensional nano-material composite has been investigated for various industrial applications due to their optical, mechanical and electrical properties.
25942815	3	27	used	used	489:492	arg2	tetraethoxysiliane					377:394	The tetraethoxysiliane	373:394	The tetraethoxysiliane (TEOS)	373:401	The tetraethoxysiliane (TEOS) and gold precursors and γ-aminopropyltriethoxysilane (γ-APTES) as coupling agent were used for sol-gel cross-linking process.
25942815	3	27	used	used	489:492	arg2	precursors					412:421	gold precursors	407:421	gold precursors	407:421	The tetraethoxysiliane (TEOS) and gold precursors and γ-aminopropyltriethoxysilane (γ-APTES) as coupling agent were used for sol-gel cross-linking process.
25942815	3	27	used	used	489:492	arg2	TEOS					397:400	TEOS	397:400	TEOS	397:400	The tetraethoxysiliane (TEOS) and gold precursors and γ-aminopropyltriethoxysilane (γ-APTES) as coupling agent were used for sol-gel cross-linking process.
25942815	2	28	theme	present	240:246	arg1	investigation					248:260	present investigation	240:260	present investigation	240:260	In present investigation, cellulose/silica and silica-gold hybrid biomaterials were prepared by sol-gel covalent cross-linking process.
25942815	4	29	theme	TEM	705:707	arg1	analysis					709:716	TEM analysis	705:716	TEM analysis	705:716	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids were confirmed by FTIR, XRD, SEM, and TEM analysis.
25942815	1	30	theme	due	177:179	arg1	applications					164:175	various industrial applications	145:175	various industrial applications due to their optical, mechanical and electrical properties	145:234	Cellulose and one dimensional nano-material composite has been investigated for various industrial applications due to their optical, mechanical and electrical properties.
25942815	5	31	theme	covalent	872:879	arg1	bonds					881:885	the covalent bonds	868:885	the covalent bonds	868:885	In the sol-gel process, the inorganic particles were dispersed in the cellulose host matrix at the nanometer scale, bonding to the cellulose through the covalent bonds.
25942815	4	32	theme	cellulose/silica-gold	595:615	arg1	nano-materials					617:630	cellulose/silica-gold nano-materials	595:630	cellulose/silica-gold nano-materials via covalent cross-linking	595:657	The chemical and morphological properties of cellulose/silica and cellulose/silica-gold nano-materials via covalent cross-linking hybrids were confirmed by FTIR, XRD, SEM, and TEM analysis.
25942815	2	33	theme	hybrid	296:301	arg1	biomaterials					303:314	silica-gold hybrid biomaterials	284:314	silica-gold hybrid biomaterials	284:314	In present investigation, cellulose/silica and silica-gold hybrid biomaterials were prepared by sol-gel covalent cross-linking process.
24479900	4	0	dep	CMC	648:650	arg1	the					644:646	the	644:646	the	644:646	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	7	1	theme	nm	1009:1010	arg1	diameter					992:999	TEM-measured particle diameter	970:999	TEM-measured particle diameter of 80.2 nm	970:1010	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	7	1	theme	nm	1009:1010	arg1	diameter					1029:1036	hydrodynamic diameter	1016:1036	hydrodynamic diameter of 562.9 nm	1016:1048	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	1	2	theme	sandy	134:138	arg1	area					151:154	a contaminated sandy subsurface area	119:154	a contaminated sandy subsurface area in Sarnia, Ontario	119:173	Nanoscale zerovalent iron (nZVI) particles were injected into a contaminated sandy subsurface area in Sarnia, Ontario.
24479900	9	3	theme	downstream	1314:1323	arg1	well					1336:1339	the downstream monitoring well	1310:1339	the downstream monitoring well	1310:1339	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	9	4	theme	X-ray	1200:1204	arg1	spectroscopy					1206:1217	Energy dispersive X-ray spectroscopy	1182:1217	Energy dispersive X-ray spectroscopy (EDS)	1182:1223	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	9	4	theme	X-ray	1200:1204	arg1	EDS					1220:1222	EDS	1220:1222	EDS	1220:1222	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	4	5	theme	nZVI	452:455	arg1	particles					457:465	Individual nZVI particles	441:465	Individual nZVI particles formed during synthesis	441:489	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	1	6	theme	subsurface	140:149	arg1	area					151:154	a contaminated sandy subsurface area	119:154	a contaminated sandy subsurface area in Sarnia, Ontario	119:173	Nanoscale zerovalent iron (nZVI) particles were injected into a contaminated sandy subsurface area in Sarnia, Ontario.
24479900	9	7	used	used	1229:1232	arg2	EDS					1220:1222	EDS	1220:1222	EDS	1220:1222	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	9	7	used	used	1229:1232	arg2	spectroscopy					1206:1217	Energy dispersive X-ray spectroscopy	1182:1217	Energy dispersive X-ray spectroscopy (EDS)	1182:1223	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	0	8	from	Characterization	0:15	arg1	test					51:54	a field scale test	37:54	a field scale test	37:54	Characterization of nZVI mobility in a field scale test.
24479900	2	9	theme	precipitation	270:282	arg1	method					284:289	the chemical precipitation method	257:289	the chemical precipitation method with sodium borohydride (NaBH4)	257:321	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	2	9	theme	precipitation	270:282	arg1	reductant					330:338	the reductant	326:338	the reductant in the presence of 0.8% wt	326:365	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	10	10	theme	Fe	1520:1521	arg1	concentrations					1526:1539	significant Fe(0) concentrations	1508:1539	significant Fe(0) concentrations for reaction with target contaminants	1508:1577	This study suggests that CMC stabilized nZVI can be transported at least 1 m to the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants.
24479900	2	11	theme	g/L	233:235	arg1	slurry					221:226	a slurry	219:226	a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt	219:365	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	6	12	theme	injection	859:867	arg1	period					869:874	the 24 h injection period	850:874	the 24 h injection period	850:874	Peak normalized total Fe breakthrough of 71% was observed 1m from the injection well and remained above 50% for the 24 h injection period.
24479900	7	13	dep	m	920:920	arg1	1					918:918	1	918:918	1	918:918	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	7	14	theme	particle	983:990	arg1	diameter					992:999	TEM-measured particle diameter	970:999	TEM-measured particle diameter of 80.2 nm	970:1010	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	8	15	theme	monitoring	1165:1174	arg1	well					1176:1179	the monitoring well	1161:1179	the monitoring well	1161:1179	No morphological changes were discernible between the injected nanoparticles and nanoparticles recovered from the monitoring well.
24479900	1	16	theme	Nanoscale	57:65	arg1	nZVI					84:87	nZVI	84:87	nZVI	84:87	Nanoscale zerovalent iron (nZVI) particles were injected into a contaminated sandy subsurface area in Sarnia, Ontario.
24479900	1	16	theme	Nanoscale	57:65	arg1	iron					78:81	Nanoscale zerovalent iron	57:81	Nanoscale zerovalent iron (nZVI) particles	57:98	Nanoscale zerovalent iron (nZVI) particles were injected into a contaminated sandy subsurface area in Sarnia, Ontario.
24479900	9	17	theme	successful	1356:1365	arg1	transport					1367:1375	the successful transport	1352:1375	the successful transport of nZVI particles	1352:1393	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	3	18	theme	sodium	368:373	arg1	CMC					399:401	CMC	399:401	CMC	399:401	sodium carboxymethylcellulose (CMC) polymer to form a stable suspension.
24479900	3	18	theme	sodium	368:373	arg1	carboxymethylcellulose					375:396	sodium carboxymethylcellulose	368:396	sodium carboxymethylcellulose (CMC) polymer	368:410	sodium carboxymethylcellulose (CMC) polymer to form a stable suspension.
24479900	10	19	theme	significant	1508:1518	arg1	Fe					1520:1521	significant Fe	1508:1521	significant Fe(0) concentrations for reaction with target contaminants	1508:1577	This study suggests that CMC stabilized nZVI can be transported at least 1 m to the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants.
24479900	10	19	theme	significant	1508:1518	arg1	0					1523:1523	0	1523:1523	0	1523:1523	This study suggests that CMC stabilized nZVI can be transported at least 1 m to the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants.
24479900	10	20	theme	target	1559:1564	arg1	contaminants					1566:1577	target contaminants	1559:1577	target contaminants	1559:1577	This study suggests that CMC stabilized nZVI can be transported at least 1 m to the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants.
24479900	1	21	theme	zerovalent	67:76	arg1	nZVI					84:87	nZVI	84:87	nZVI	84:87	Nanoscale zerovalent iron (nZVI) particles were injected into a contaminated sandy subsurface area in Sarnia, Ontario.
24479900	1	21	theme	zerovalent	67:76	arg1	iron					78:81	Nanoscale zerovalent iron	57:81	Nanoscale zerovalent iron (nZVI) particles	57:98	Nanoscale zerovalent iron (nZVI) particles were injected into a contaminated sandy subsurface area in Sarnia, Ontario.
24479900	3	22	theme	carboxymethylcellulose	375:396	arg1	polymer					404:410	sodium carboxymethylcellulose (CMC) polymer	368:410	sodium carboxymethylcellulose (CMC) polymer	368:410	sodium carboxymethylcellulose (CMC) polymer to form a stable suspension.
24479900	4	23	theme	transmission	497:508	arg1	TEM					531:533	TEM	531:533	TEM	531:533	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	23	theme	transmission	497:508	arg1	microscopy					519:528	a transmission electron microscopy	495:528	a transmission electron microscopy (TEM) quantified particle size of 86.0 nm	495:570	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	1	24	theme	iron	78:81	arg1	particles					90:98	Nanoscale zerovalent iron (nZVI) particles	57:98	Nanoscale zerovalent iron (nZVI) particles	57:98	Nanoscale zerovalent iron (nZVI) particles were injected into a contaminated sandy subsurface area in Sarnia, Ontario.
24479900	7	25	dep	monitoring	902:911	arg1	m					920:920	m	920:920	m	920:920	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	9	26	theme	nZVI	1380:1383	arg1	particles					1385:1393	nZVI particles	1380:1393	nZVI particles	1380:1393	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	5	27	theme	gravity	719:725	arg1	injection					727:735	gravity injection	719:735	gravity injection	719:735	The nZVI was delivered to the subsurface via gravity injection.
24479900	6	28	theme	h	857:857	arg1	period					869:874	the 24 h injection period	850:874	the 24 h injection period	850:874	Peak normalized total Fe breakthrough of 71% was observed 1m from the injection well and remained above 50% for the 24 h injection period.
24479900	7	29	theme	TEM-measured	970:981	arg1	diameter					992:999	TEM-measured particle diameter	970:999	TEM-measured particle diameter of 80.2 nm	970:1010	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	2	30	theme	chemical	261:268	arg1	method					284:289	the chemical precipitation method	257:289	the chemical precipitation method with sodium borohydride (NaBH4)	257:321	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	2	30	theme	chemical	261:268	arg1	reductant					330:338	the reductant	326:338	the reductant in the presence of 0.8% wt	326:365	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	0	31	theme	mobility	25:32	arg1	Characterization					0:15	Characterization	0:15	Characterization of nZVI mobility in a field scale test.	0:55	Characterization of nZVI mobility in a field scale test.
24479900	6	32	theme	24	854:855	arg1	h					857:857	h	857:857	h	857:857	Peak normalized total Fe breakthrough of 71% was observed 1m from the injection well and remained above 50% for the 24 h injection period.
24479900	4	33	contain	had	491:493	arg2	DLS					602:604	DLS	602:604	DLS	602:604	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	33	contain	had	491:493	arg2	microscopy					519:528	a transmission electron microscopy	495:528	a transmission electron microscopy (TEM) quantified particle size of 86.0 nm	495:570	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	33	contain	had	491:493	arg1	particles					457:465	Individual nZVI particles	441:465	Individual nZVI particles formed during synthesis	441:489	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	33	contain	had	491:493	arg2	scattering					590:599	dynamic light scattering	576:599	dynamic light scattering (DLS)	576:605	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	33	contain	had	491:493	arg2	TEM					531:533	TEM	531:533	TEM	531:533	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	2	34	theme	%	362:362	arg1	wt					364:365	0.8% wt	359:365	0.8% wt	359:365	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	2	35	theme	wt	364:365	arg1	presence					347:354	the presence	343:354	the presence of 0.8% wt	343:365	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	4	36	theme	electron	510:517	arg1	TEM					531:533	TEM	531:533	TEM	531:533	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	36	theme	electron	510:517	arg1	microscopy					519:528	a transmission electron microscopy	495:528	a transmission electron microscopy (TEM) quantified particle size of 86.0 nm	495:570	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	6	37	theme	%	781:781	arg1	breakthrough					763:774	total Fe breakthrough	754:774	total Fe breakthrough of 71%	754:781	Peak normalized total Fe breakthrough of 71% was observed 1m from the injection well and remained above 50% for the 24 h injection period.
24479900	4	38	theme	hydrodynamic	618:629	arg1	diameter					631:638	quantified hydrodynamic diameter	607:638	quantified hydrodynamic diameter	607:638	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	2	39	theme	0.8	359:361	arg1	%					362:362	%	362:362	%	362:362	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	8	40	theme	injected	1105:1112	arg1	nanoparticles					1114:1126	the injected nanoparticles	1101:1126	the injected nanoparticles	1101:1126	No morphological changes were discernible between the injected nanoparticles and nanoparticles recovered from the monitoring well.
24479900	3	41	theme	stable	422:427	arg1	suspension					429:438	a stable suspension	420:438	a stable suspension	420:438	sodium carboxymethylcellulose (CMC) polymer to form a stable suspension.
24479900	2	42	with	method	284:289	arg1	NaBH4					316:320	NaBH4	316:320	NaBH4	316:320	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	2	42	with	method	284:289	arg1	borohydride					303:313	sodium borohydride	296:313	sodium borohydride (NaBH4)	296:321	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	9	43	theme	elemental	1249:1257	arg1	composition					1259:1269	the elemental composition	1245:1269	the elemental composition of the iron nanoparticles sampled from the downstream monitoring well	1245:1339	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	7	44	contain	contained	941:949	arg2	nanoparticles					951:963	nanoparticles	951:963	nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm	951:1048	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	7	44	contain	contained	941:949	arg1	Samples					877:883	Samples	877:883	Samples collected from a monitoring well 1 m from the injection	877:939	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	4	45	theme	dynamic	576:582	arg1	scattering					590:599	dynamic light scattering	576:599	dynamic light scattering (DLS)	576:605	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	45	theme	dynamic	576:582	arg1	DLS					602:604	DLS	602:604	DLS	602:604	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	9	46	theme	nanoparticles	1283:1295	arg1	composition					1259:1269	the elemental composition	1245:1269	the elemental composition of the iron nanoparticles sampled from the downstream monitoring well	1245:1339	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	10	47	with	reaction	1545:1552	arg1	contaminants					1566:1577	target contaminants	1559:1577	target contaminants	1559:1577	This study suggests that CMC stabilized nZVI can be transported at least 1 m to the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants.
24479900	9	48	theme	monitoring	1325:1334	arg1	well					1336:1339	the downstream monitoring well	1310:1339	the downstream monitoring well	1310:1339	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	7	49	theme	nm	1047:1048	arg1	diameter					992:999	TEM-measured particle diameter	970:999	TEM-measured particle diameter of 80.2 nm	970:1010	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	7	49	theme	nm	1047:1048	arg1	diameter					1029:1036	hydrodynamic diameter	1016:1036	hydrodynamic diameter of 562.9 nm	1016:1048	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	2	50	from	reductant	330:338	arg1	presence					347:354	the presence	343:354	the presence of 0.8% wt	343:365	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	7	51	with	nanoparticles	951:963	arg1	diameter					992:999	TEM-measured particle diameter	970:999	TEM-measured particle diameter of 80.2 nm	970:1010	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	7	51	with	nanoparticles	951:963	arg1	diameter					1029:1036	hydrodynamic diameter	1016:1036	hydrodynamic diameter of 562.9 nm	1016:1048	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	0	52	theme	scale	45:49	arg1	test					51:54	a field scale test	37:54	a field scale test	37:54	Characterization of nZVI mobility in a field scale test.
24479900	4	53	dep	microscopy	519:528	arg1	diameter					631:638	quantified hydrodynamic diameter	607:638	quantified hydrodynamic diameter	607:638	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	9	54	theme	particles	1385:1393	arg1	transport					1367:1375	the successful transport	1352:1375	the successful transport of nZVI particles	1352:1393	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	10	55	dep	CMC	1421:1423	arg1	stabilized					1425:1434	stabilized	1425:1434	stabilized	1425:1434	This study suggests that CMC stabilized nZVI can be transported at least 1 m to the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants.
24479900	0	56	theme	field	39:43	arg1	test					51:54	a field scale test	37:54	a field scale test	37:54	Characterization of nZVI mobility in a field scale test.
24479900	9	57	theme	dispersive	1189:1198	arg1	spectroscopy					1206:1217	Energy dispersive X-ray spectroscopy	1182:1217	Energy dispersive X-ray spectroscopy (EDS)	1182:1223	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	9	57	theme	dispersive	1189:1198	arg1	EDS					1220:1222	EDS	1220:1222	EDS	1220:1222	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	6	58	theme	Fe	760:761	arg1	breakthrough					763:774	total Fe breakthrough	754:774	total Fe breakthrough of 71%	754:781	Peak normalized total Fe breakthrough of 71% was observed 1m from the injection well and remained above 50% for the 24 h injection period.
24479900	2	59	theme	sodium	296:301	arg1	NaBH4					316:320	NaBH4	316:320	NaBH4	316:320	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	2	59	theme	sodium	296:301	arg1	borohydride					303:313	sodium borohydride	296:313	sodium borohydride (NaBH4)	296:321	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	9	60	theme	iron	1278:1281	arg1	nanoparticles					1283:1295	the iron nanoparticles	1274:1295	the iron nanoparticles sampled from the downstream monitoring well	1274:1339	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	6	61	theme	total	754:758	arg1	breakthrough					763:774	total Fe breakthrough	754:774	total Fe breakthrough of 71%	754:781	Peak normalized total Fe breakthrough of 71% was observed 1m from the injection well and remained above 50% for the 24 h injection period.
24479900	2	62	theme	slurry	221:226	arg1	nanoparticles					237:249	a slurry of 1 g/L nanoparticles	219:249	a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt	219:365	The nZVI was synthesized on site, creating a slurry of 1 g/L nanoparticles using the chemical precipitation method with sodium borohydride (NaBH4) as the reductant in the presence of 0.8% wt.
24479900	10	63	theme	contaminated	1480:1491	arg1	zone					1500:1503	the contaminated source zone	1476:1503	the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants	1476:1577	This study suggests that CMC stabilized nZVI can be transported at least 1 m to the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants.
24479900	4	64	theme	nm	670:671	arg1	nZVI					656:659	nZVI	656:659	nZVI	656:659	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	64	theme	nm	670:671	arg1	CMC					648:650	CMC	648:650	CMC	648:650	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	65	theme	particle	547:554	arg1	size					556:559	particle size	547:559	particle size of 86.0 nm	547:570	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	10	66	theme	CMC	1421:1423	arg1	nZVI					1436:1439	CMC stabilized nZVI	1421:1439	CMC stabilized nZVI	1421:1439	This study suggests that CMC stabilized nZVI can be transported at least 1 m to the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants.
24479900	4	67	theme	Individual	441:450	arg1	particles					457:465	Individual nZVI particles	441:465	Individual nZVI particles formed during synthesis	441:489	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	10	68	from	concentrations	1526:1539	arg1	zone					1500:1503	the contaminated source zone	1476:1503	the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants	1476:1577	This study suggests that CMC stabilized nZVI can be transported at least 1 m to the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants.
24479900	6	69	from	injection	808:816	arg1	1m					796:797	1m	796:797	1m	796:797	Peak normalized total Fe breakthrough of 71% was observed 1m from the injection well and remained above 50% for the 24 h injection period.
24479900	9	70	theme	Energy	1182:1187	arg1	spectroscopy					1206:1217	Energy dispersive X-ray spectroscopy	1182:1217	Energy dispersive X-ray spectroscopy (EDS)	1182:1223	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	9	70	theme	Energy	1182:1187	arg1	EDS					1220:1222	EDS	1220:1222	EDS	1220:1222	Energy dispersive X-ray spectroscopy (EDS) was used to confirm the elemental composition of the iron nanoparticles sampled from the downstream monitoring well, verifying the successful transport of nZVI particles.
24479900	10	71	theme	source	1493:1498	arg1	zone					1500:1503	the contaminated source zone	1476:1503	the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants	1476:1577	This study suggests that CMC stabilized nZVI can be transported at least 1 m to the contaminated source zone at significant Fe(0) concentrations for reaction with target contaminants.
24479900	4	72	theme	nm	569:570	arg1	size					556:559	particle size	547:559	particle size of 86.0 nm	547:570	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	73	theme	quantified	607:616	arg1	diameter					631:638	quantified hydrodynamic diameter	607:638	quantified hydrodynamic diameter	607:638	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	74	theme	light	584:588	arg1	scattering					590:599	dynamic light scattering	576:599	dynamic light scattering (DLS)	576:605	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	4	74	theme	light	584:588	arg1	DLS					602:604	DLS	602:604	DLS	602:604	Individual nZVI particles formed during synthesis had a transmission electron microscopy (TEM) quantified particle size of 86.0 nm and dynamic light scattering (DLS) quantified hydrodynamic diameter for the CMC and nZVI of 624.8 nm.
24479900	7	75	theme	hydrodynamic	1016:1027	arg1	diameter					1029:1036	hydrodynamic diameter	1016:1036	hydrodynamic diameter of 562.9 nm	1016:1048	Samples collected from a monitoring well 1 m from the injection contained nanoparticles with TEM-measured particle diameter of 80.2 nm and hydrodynamic diameter of 562.9 nm.
24479900	8	76	theme	morphological	1054:1066	arg1	changes					1068:1074	No morphological changes	1051:1074	No morphological changes	1051:1074	No morphological changes were discernible between the injected nanoparticles and nanoparticles recovered from the monitoring well.
24479900	0	77	theme	nZVI	20:23	arg1	mobility					25:32	nZVI mobility	20:32	nZVI mobility	20:32	Characterization of nZVI mobility in a field scale test.
24479900	1	78	from	area	151:154	arg1	Ontario					167:173	Ontario	167:173	Ontario	167:173	Nanoscale zerovalent iron (nZVI) particles were injected into a contaminated sandy subsurface area in Sarnia, Ontario.
24479900	1	79	theme	contaminated	121:132	arg1	area					151:154	a contaminated sandy subsurface area	119:154	a contaminated sandy subsurface area in Sarnia, Ontario	119:173	Nanoscale zerovalent iron (nZVI) particles were injected into a contaminated sandy subsurface area in Sarnia, Ontario.
27337563	0	0	theme	Cell	96:99	arg1	Wall					101:104	the Cell Wall	92:104	the Cell Wall	92:104	Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity.
27337563	4	1	theme	data	621:624	arg1	analysis					626:633	multivariate data analysis	608:633	multivariate data analysis	608:633	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	4	2	theme	bacteria	738:745	arg1	bacteria					738:745	Gram negative bacteria	724:745	Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles	724:826	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	4	2	theme	bacteria	738:745	arg1	Alphaproteobacteria					692:710	the Class Alphaproteobacteria	682:710	the Class Alphaproteobacteria	682:710	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	4	2	theme	bacteria	738:745	arg1	group					715:719	a group	713:719	a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles	713:826	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	4	3	theme	multivariate	608:619	arg1	analysis					626:633	multivariate data analysis	608:633	multivariate data analysis	608:633	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	3	4	theme	variability	379:389	arg1	degree					369:374	a significant degree	355:374	a significant degree of variability	355:389	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	7	5	theme	large	1443:1447	arg1	sets					1480:1483	large cell wall-chromatographic data sets	1443:1483	large cell wall-chromatographic data sets	1443:1483	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	3	6	dep	composition	407:417	arg1	the					394:396	the	394:396	the	394:396	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	5	7	from	-carboxyl	948:956	arg1	presence					994:1001	the presence	990:1001	the presence of muropeptides	990:1017	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	5	7	from	-carboxyl	948:956	arg1	amidation					926:934	amidation	926:934	amidation at the α-(l)-carboxyl of meso-diaminopimelic acid	926:984	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	5	8	theme	muropeptides	1006:1017	arg1	presence					994:1001	the presence	990:1001	the presence of muropeptides	990:1017	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	5	8	theme	muropeptides	1006:1017	arg1	amidation					926:934	amidation	926:934	amidation at the α-(l)-carboxyl of meso-diaminopimelic acid	926:984	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	4	9	theme	morphology	801:810	arg1	terms					780:784	terms	780:784	terms of metabolism, morphology and life-styles	780:826	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	2	10	theme	environmental	296:308	arg1	insults					310:316	environmental insults	296:316	environmental insults	296:316	It contributes to the cell morphology and provides cell wall integrity against environmental insults.
27337563	7	11	theme	structural	1517:1526	arg1	properties					1528:1537	novel peptidoglycan structural properties	1497:1537	novel peptidoglycan structural properties in bacteria	1497:1549	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	7	12	theme	chemometric	1422:1432	arg1	tools					1434:1438	chemometric tools	1422:1438	chemometric tools on large cell wall-chromatographic data sets	1422:1483	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	6	13	theme	immune	1262:1267	arg1	system					1269:1274	the Drosophila innate immune system	1240:1274	the Drosophila innate immune system	1240:1274	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	7	14	theme	wall-chromatographic	1454:1473	arg1	sets					1480:1483	large cell wall-chromatographic data sets	1443:1483	large cell wall-chromatographic data sets	1443:1483	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	1	15	theme	fundamental	176:186	arg1	Peptidoglycan					157:169	Peptidoglycan	157:169	Peptidoglycan	157:169	Peptidoglycan is a fundamental structure for most bacteria.
27337563	1	15	theme	fundamental	176:186	arg1	structure					188:196	a fundamental structure	174:196	a fundamental structure for most bacteria	174:214	Peptidoglycan is a fundamental structure for most bacteria.
27337563	0	16	theme	Predatory	114:122	arg1	Enzymes					124:130	Predatory Enzymes	114:130	Predatory Enzymes	114:130	Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity.
27337563	3	17	from	composition	407:417	arg1	Bacteria					467:474	the domain Bacteria	456:474	the domain Bacteria	456:474	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	4	18	theme	life-styles	816:826	arg1	terms					780:784	terms	780:784	terms of metabolism, morphology and life-styles	780:826	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	7	19	from	sets	1480:1483	arg1	power					1413:1417	the discriminative power	1394:1417	the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria	1394:1549	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	7	20	theme	cell	1449:1452	arg1	sets					1480:1483	large cell wall-chromatographic data sets	1443:1483	large cell wall-chromatographic data sets	1443:1483	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	6	21	theme	bacteria	1346:1353	arg1	adaptability					1324:1335	the environmental adaptability	1306:1335	the environmental adaptability of these bacteria	1306:1353	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	6	22	theme	Drosophila	1244:1253	arg1	system					1269:1274	the Drosophila innate immune system	1240:1274	the Drosophila innate immune system	1240:1274	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	7	23	theme	tools	1434:1438	arg1	power					1413:1417	the discriminative power	1394:1417	the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria	1394:1549	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	0	24	theme	Chemometric	0:10	arg1	Analysis					12:19	Chemometric Analysis	0:19	Chemometric Analysis of Bacterial Peptidoglycan	0:46	Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity.
27337563	4	25	theme	liquid	582:587	arg1	chromatography					589:602	rapid ultraperformance liquid chromatography	559:602	rapid ultraperformance liquid chromatography	559:602	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	5	26	theme	novel	867:871	arg1	structures					887:896	novel peptidoglycan structures	867:896	novel peptidoglycan structures conserved in Acetobacteria	867:923	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	3	27	theme	domain	460:465	arg1	Bacteria					467:474	the domain Bacteria	456:474	the domain Bacteria	456:474	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	0	28	theme	Bacterial	24:32	arg1	Peptidoglycan					34:46	Bacterial Peptidoglycan	24:46	Bacterial Peptidoglycan	24:46	Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity.
27337563	4	29	theme	ultraperformance	565:580	arg1	chromatography					589:602	rapid ultraperformance liquid chromatography	559:602	rapid ultraperformance liquid chromatography	559:602	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	0	30	theme	Fly	136:138	arg1	Immunity					147:154	Fly Innate Immunity	136:154	Fly Innate Immunity	136:154	Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity.
27337563	4	31	from	terms	780:784	arg1	heterogeneous					763:775	heterogeneous	763:775	heterogeneous	763:775	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	7	32	theme	peptidoglycan	1503:1515	arg1	properties					1528:1537	novel peptidoglycan structural properties	1497:1537	novel peptidoglycan structural properties in bacteria	1497:1549	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	6	33	theme	growth-controlled	1105:1121	arg1	structures					1090:1099	Both structures	1085:1099	Both structures	1085:1099	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	6	33	theme	growth-controlled	1105:1121	arg1	modifications					1123:1135	growth-controlled modifications	1105:1135	growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria	1105:1353	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	3	34	theme	chemical	398:405	arg1	composition					407:417	chemical composition	398:417	chemical composition	398:417	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	4	35	theme	metabolism	789:798	arg1	terms					780:784	terms	780:784	terms of metabolism, morphology and life-styles	780:826	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	5	36	theme	peptidoglycan	873:885	arg1	structures					887:896	novel peptidoglycan structures	867:896	novel peptidoglycan structures conserved in Acetobacteria	867:923	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	3	37	theme	peptidoglycan	439:451	arg1	composition					407:417	chemical composition	398:417	chemical composition	398:417	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	3	37	theme	peptidoglycan	439:451	arg1	organization					423:434	organization	423:434	organization	423:434	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	6	38	dep	Type	1167:1170	arg1	VI					1172:1173	VI	1172:1173	VI	1172:1173	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	6	38	dep	Type	1167:1170	arg1	endopeptidases					1206:1219	peptidoglycan endopeptidases	1192:1219	Type VI secretion system peptidoglycan endopeptidases	1167:1219	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	6	38	dep	Type	1167:1170	arg1	system					1185:1190	secretion system	1175:1190	Type VI secretion system peptidoglycan endopeptidases	1167:1219	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	1	39	theme	most	202:205	arg1	bacteria					207:214	most bacteria	202:214	most bacteria	202:214	Peptidoglycan is a fundamental structure for most bacteria.
27337563	7	40	theme	discriminative	1398:1411	arg1	power					1413:1417	the discriminative power	1394:1417	the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria	1394:1549	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	0	41	theme	Peptidoglycan	34:46	arg1	Analysis					12:19	Chemometric Analysis	0:19	Chemometric Analysis of Bacterial Peptidoglycan	0:46	Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity.
27337563	5	42	attach	presence	994:1001	arg1	-carboxyl					948:956	the α-(l)-carboxyl	939:956	the α-(l)-carboxyl of meso-diaminopimelic acid	939:984	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	5	42	attach	presence	994:1001	arg2	muropeptides					1006:1017	muropeptides	1006:1017	muropeptides	1006:1017	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	5	42	attach	presence	994:1001	arg1	l					946:946	l	946:946	l	946:946	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	0	43	theme	Innate	140:145	arg1	Immunity					147:154	Fly Innate Immunity	136:154	Fly Innate Immunity	136:154	Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity.
27337563	2	44	theme	cell	239:242	arg1	morphology					244:253	the cell morphology	235:253	the cell morphology	235:253	It contributes to the cell morphology and provides cell wall integrity against environmental insults.
27337563	3	45	theme	real	481:484	arg1	diversity					486:494	the real diversity	477:494	the real diversity of this polymer	477:510	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	5	46	theme	chemometric	837:847	arg1	analyses					849:856	chemometric analyses	837:856	chemometric analyses	837:856	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	3	47	theme	several	325:331	arg1	studies					333:339	several studies	325:339	several studies	325:339	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	6	48	theme	environmental	1310:1322	arg1	adaptability					1324:1335	the environmental adaptability	1306:1335	the environmental adaptability of these bacteria	1306:1353	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	6	49	theme	secretion	1175:1183	arg1	system					1185:1190	secretion system	1175:1190	Type VI secretion system peptidoglycan endopeptidases	1167:1219	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	0	50	theme	Atypical	56:63	arg1	Modifications					65:77	Atypical Modifications	56:77	Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity	56:154	Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity.
27337563	4	51	theme	rapid	559:563	arg1	chromatography					589:602	rapid ultraperformance liquid chromatography	559:602	rapid ultraperformance liquid chromatography	559:602	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	7	52	theme	novel	1497:1501	arg1	properties					1528:1537	novel peptidoglycan structural properties	1497:1537	novel peptidoglycan structural properties in bacteria	1497:1549	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	5	53	theme	meso-diaminopimelic	961:979	arg1	acid					981:984	meso-diaminopimelic acid	961:984	meso-diaminopimelic acid	961:984	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	7	54	from	properties	1528:1537	arg1	bacteria					1542:1549	bacteria	1542:1549	bacteria	1542:1549	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	5	55	theme	α-	943:944	arg1	-carboxyl					948:956	the α-(l)-carboxyl	939:956	the α-(l)-carboxyl of meso-diaminopimelic acid	939:984	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	5	55	theme	α-	943:944	arg1	l					946:946	l	946:946	l	946:946	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	2	56	theme	wall	273:276	arg1	integrity					278:286	cell wall integrity	268:286	cell wall integrity against environmental insults	268:316	It contributes to the cell morphology and provides cell wall integrity against environmental insults.
27337563	5	57	theme	-diaminopimelate	1055:1070	arg1	cross-links					1072:1082	(1-3) l-Ala-d-(meso)-diaminopimelate cross-links	1035:1082	(1-3) l-Ala-d-(meso)-diaminopimelate cross-links	1035:1082	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	5	58	theme	acid	981:984	arg1	-carboxyl					948:956	the α-(l)-carboxyl	939:956	the α-(l)-carboxyl of meso-diaminopimelic acid	939:984	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	5	58	theme	acid	981:984	arg1	l					946:946	l	946:946	l	946:946	Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(l)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) l-Ala-d-(meso)-diaminopimelate cross-links.
27337563	4	59	from	heterogeneous	763:775	arg1	terms					780:784	terms	780:784	terms of metabolism, morphology and life-styles	780:826	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	6	60	theme	innate	1255:1260	arg1	system					1269:1274	the Drosophila innate immune system	1240:1274	the Drosophila innate immune system	1240:1274	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	2	61	theme	cell	268:271	arg1	integrity					278:286	cell wall integrity	268:286	cell wall integrity against environmental insults	268:316	It contributes to the cell morphology and provides cell wall integrity against environmental insults.
27337563	7	62	from	tools	1434:1438	arg1	sets					1480:1483	large cell wall-chromatographic data sets	1443:1483	large cell wall-chromatographic data sets	1443:1483	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	3	63	theme	polymer	504:510	arg1	diversity					486:494	the real diversity	477:494	the real diversity of this polymer	477:510	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	6	64	theme	peptidoglycan	1192:1204	arg1	endopeptidases					1206:1219	peptidoglycan endopeptidases	1192:1219	Type VI secretion system peptidoglycan endopeptidases	1167:1219	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	7	65	from	power	1413:1417	arg1	sets					1480:1483	large cell wall-chromatographic data sets	1443:1483	large cell wall-chromatographic data sets	1443:1483	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	4	66	theme	Class	686:690	arg1	bacteria					738:745	Gram negative bacteria	724:745	Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles	724:826	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	4	66	theme	Class	686:690	arg1	Alphaproteobacteria					692:710	the Class Alphaproteobacteria	682:710	the Class Alphaproteobacteria	682:710	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	4	66	theme	Class	686:690	arg1	group					715:719	a group	713:719	a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles	713:826	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	6	67	theme	relevant	1288:1295	arg1	roles					1297:1301	relevant roles	1288:1301	relevant roles	1288:1301	Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria.
27337563	4	68	theme	negative	729:736	arg1	bacteria					738:745	Gram negative bacteria	724:745	Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles	724:826	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	3	69	theme	significant	357:367	arg1	degree					369:374	a significant degree	355:374	a significant degree of variability	355:389	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	3	70	from	organization	423:434	arg1	Bacteria					467:474	the domain Bacteria	456:474	the domain Bacteria	456:474	While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored.
27337563	7	71	theme	data	1475:1478	arg1	sets					1480:1483	large cell wall-chromatographic data sets	1443:1483	large cell wall-chromatographic data sets	1443:1483	Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
27337563	4	72	theme	Gram	724:727	arg1	bacteria					738:745	Gram negative bacteria	724:745	Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles	724:826	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
27337563	4	73	theme	chemical	660:667	arg1	diversity					669:677	peptidoglycan chemical diversity	646:677	peptidoglycan chemical diversity	646:677	This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles.
26156050	5	0	from	groups	837:842	arg1	C					821:821	δ(13)C	816:821	δ(13)C from methoxyl groups	816:842	Compared with the whole wood samples, δ(13)C from methoxyl groups showed a different and overall lower response to climate parameters.
26156050	5	1	theme	lower	875:879	arg1	response					881:888	a different and overall lower response	851:888	a different and overall lower response to climate parameters	851:910	Compared with the whole wood samples, δ(13)C from methoxyl groups showed a different and overall lower response to climate parameters.
26156050	3	2	from	groups	579:584	arg1	O					507:507	δ(18)O	502:507	δ(18)O	502:507	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	3	2	from	groups	579:584	arg1	C					553:553	(ii) δ(13)C	543:553	(ii) δ(13)C	543:553	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	3	2	from	groups	579:584	arg1	H					563:563	δ(2)H	559:563	δ(2)H	559:563	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	3	2	from	groups	579:584	arg1	C					496:496	(i) δ(13)C	487:496	(i) δ(13)C	487:496	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	4	3	theme	methoxyl	761:768	arg1	groups					770:775	lignin methoxyl groups	754:775	lignin methoxyl groups	754:775	Results indicate significant correlations among the time series derived from whole wood, cellulose, and lignin methoxyl groups.
26156050	1	4	theme	methoxyl	175:182	arg1	groups					184:189	methoxyl groups	175:189	methoxyl groups	175:189	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	8	5	theme	different	1546:1554	arg1	components					1561:1570	different wood components	1546:1570	different wood components	1546:1570	These findings reveal that multiple climate elements can be reconstructed from different wood components and that whole wood proxies perform comparably to cellulose time series.
26156050	7	6	theme	Isotope	1091:1097	arg1	series					1104:1109	Isotope time series	1091:1109	Isotope time series with the highest correlation with climatic parameter	1091:1162	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	4	7	attach	derived	714:720	arg2	series					707:712	the time series	698:712	the time series derived from whole wood, cellulose, and lignin methoxyl groups	698:775	Results indicate significant correlations among the time series derived from whole wood, cellulose, and lignin methoxyl groups.
26156050	4	7	attach	derived	714:720	arg1	groups					770:775	lignin methoxyl groups	754:775	lignin methoxyl groups	754:775	Results indicate significant correlations among the time series derived from whole wood, cellulose, and lignin methoxyl groups.
26156050	4	7	attach	derived	714:720	arg1	cellulose					739:747	cellulose	739:747	cellulose	739:747	Results indicate significant correlations among the time series derived from whole wood, cellulose, and lignin methoxyl groups.
26156050	4	7	attach	derived	714:720	arg1	wood					733:736	whole wood	727:736	whole wood	727:736	Results indicate significant correlations among the time series derived from whole wood, cellulose, and lignin methoxyl groups.
26156050	1	8	theme	δ	153:153	arg1	parameters					140:149	Novel tree ring parameters	124:149	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups -	124:191	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	8	theme	δ	153:153	arg1	C					158:158	δ(13)C	153:158	δ(13)C	153:158	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	7	9	theme	δ	1343:1343	arg1	O					1348:1348	cellulose δ(18)O	1333:1348	cellulose δ(18)O	1333:1348	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	10	theme	season	1219:1224	arg1	precipitation					1226:1238	growing season precipitation	1211:1238	growing season precipitation	1211:1238	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	11	dep	groups	1278:1283	arg1	ii					1265:1266	ii	1265:1266	ii	1265:1266	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	12	with	H	1439:1439	arg1	temperatures					1453:1464	spring temperatures	1446:1464	spring temperatures	1446:1464	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	6	13	theme	methoxyl	943:950	arg1	groups					952:957	methoxyl groups	943:957	methoxyl groups	943:957	On the other hand, δ(2)H from methoxyl groups showed high correlations with temperature and was also correlated with ring width, indicating its potential as a temperature proxy.
26156050	2	14	theme	extracted	415:423	arg1	component					430:438	the extracted wood component	411:438	the extracted wood component	411:438	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	7	15	with	groups	1278:1283	arg1	precipitation					1297:1309	spring precipitation	1290:1309	spring precipitation	1290:1309	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	1	16	dep	H	168:168	arg1	2					166:166	2	166:166	2	166:166	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	6	17	theme	ring	1030:1033	arg1	width					1035:1039	ring width	1030:1039	ring width	1030:1039	On the other hand, δ(2)H from methoxyl groups showed high correlations with temperature and was also correlated with ring width, indicating its potential as a temperature proxy.
26156050	5	18	theme	δ	816:816	arg1	C					821:821	δ(13)C	816:821	δ(13)C from methoxyl groups	816:842	Compared with the whole wood samples, δ(13)C from methoxyl groups showed a different and overall lower response to climate parameters.
26156050	4	19	theme	time	702:705	arg1	series					707:712	the time series	698:712	the time series derived from whole wood, cellulose, and lignin methoxyl groups	698:775	Results indicate significant correlations among the time series derived from whole wood, cellulose, and lignin methoxyl groups.
26156050	0	20	from	δ¹³C	5:8	arg1	Pinus					78:82	Pinus	78:82	Pinus	78:82	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	8	21	theme	climate	1503:1509	arg1	elements					1511:1518	multiple climate elements	1494:1518	multiple climate elements	1494:1518	These findings reveal that multiple climate elements can be reconstructed from different wood components and that whole wood proxies perform comparably to cellulose time series.
26156050	7	22	with	C	1204:1204	arg1	precipitation					1226:1238	growing season precipitation	1211:1238	growing season precipitation	1211:1238	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	22	with	C	1204:1204	arg1	temperature					1251:1261	summer temperature	1244:1261	summer temperature	1244:1261	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	1	23	theme	tree	130:133	arg1	H					168:168	δ(2)H	164:168	δ(2)H	164:168	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	23	theme	tree	130:133	arg1	parameters					140:149	Novel tree ring parameters	124:149	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups -	124:191	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	23	theme	tree	130:133	arg1	C					158:158	δ(13)C	153:158	δ(13)C	153:158	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	7	24	theme	cellulose	1189:1197	arg1	C					1204:1204	cellulose δ(13)C	1189:1204	cellulose δ(13)C	1189:1204	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	2	25	theme	climate	382:388	arg1	signal					390:395	climate signal	382:395	climate signal	382:395	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	0	26	from	δ²H	0:2	arg1	Pinus					78:82	Pinus	78:82	Pinus	78:82	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	0	27	theme	methoxyl	59:66	arg1	groups					68:73	whole wood, α-cellulose and lignin methoxyl groups	24:73	whole wood, α-cellulose and lignin methoxyl groups	24:73	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	5	28	theme	whole	796:800	arg1	samples					807:813	the whole wood samples	792:813	the whole wood samples	792:813	Compared with the whole wood samples, δ(13)C from methoxyl groups showed a different and overall lower response to climate parameters.
26156050	4	29	theme	significant	667:677	arg1	correlations					679:690	significant correlations	667:690	significant correlations among the time series derived from whole wood, cellulose, and lignin methoxyl groups	667:775	Results indicate significant correlations among the time series derived from whole wood, cellulose, and lignin methoxyl groups.
26156050	3	30	from	wood	520:523	arg1	O					507:507	δ(18)O	502:507	δ(18)O	502:507	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	3	30	from	wood	520:523	arg1	C					553:553	(ii) δ(13)C	543:553	(ii) δ(13)C	543:553	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	3	30	from	wood	520:523	arg1	H					563:563	δ(2)H	559:563	δ(2)H	559:563	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	3	30	from	wood	520:523	arg1	C					496:496	(i) δ(13)C	487:496	(i) δ(13)C	487:496	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	3	31	dep	C	496:496	arg1	i					488:488	i	488:488	i	488:488	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	7	32	theme	methoxyl	1269:1276	arg1	groups					1278:1283	(ii) methoxyl groups	1264:1283	(ii) methoxyl groups with spring precipitation	1264:1309	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	2	33	theme	cellulose	300:308	arg1	samples					310:316	cellulose samples	300:316	cellulose samples	300:316	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	0	34	from	δ¹⁸O	14:17	arg1	Pinus					78:82	Pinus	78:82	Pinus	78:82	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	3	35	from	cellulose	529:537	arg1	O					507:507	δ(18)O	502:507	δ(18)O	502:507	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	3	35	from	cellulose	529:537	arg1	C					553:553	(ii) δ(13)C	543:553	(ii) δ(13)C	543:553	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	3	35	from	cellulose	529:537	arg1	H					563:563	δ(2)H	559:563	δ(2)H	559:563	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	3	35	from	cellulose	529:537	arg1	C					496:496	(i) δ(13)C	487:496	(i) δ(13)C	487:496	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	8	36	theme	multiple	1494:1501	arg1	elements					1511:1518	multiple climate elements	1494:1518	multiple climate elements	1494:1518	These findings reveal that multiple climate elements can be reconstructed from different wood components and that whole wood proxies perform comparably to cellulose time series.
26156050	6	37	theme	temperature	1072:1082	arg1	proxy					1084:1088	a temperature proxy	1070:1088	a temperature proxy	1070:1088	On the other hand, δ(2)H from methoxyl groups showed high correlations with temperature and was also correlated with ring width, indicating its potential as a temperature proxy.
26156050	5	38	theme	different	853:861	arg1	response					881:888	a different and overall lower response	851:888	a different and overall lower response to climate parameters	851:910	Compared with the whole wood samples, δ(13)C from methoxyl groups showed a different and overall lower response to climate parameters.
26156050	2	39	with	Tests	243:247	arg1	O					270:270	δ(18)O	265:270	δ(18)O derived from whole wood	265:294	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	2	39	with	Tests	243:247	arg1	C					259:259	δ(13)C	254:259	δ(13)C	254:259	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	8	40	theme	time	1632:1635	arg1	series					1637:1642	cellulose time series	1622:1642	cellulose time series	1622:1642	These findings reveal that multiple climate elements can be reconstructed from different wood components and that whole wood proxies perform comparably to cellulose time series.
26156050	7	41	dep	H	1439:1439	arg1	iv					1416:1417	iv	1416:1417	iv	1416:1417	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	3	42	theme	δ	491:491	arg1	C					496:496	(i) δ(13)C	487:496	(i) δ(13)C	487:496	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	4	43	theme	whole	727:731	arg1	wood					733:736	whole wood	727:736	whole wood	727:736	Results indicate significant correlations among the time series derived from whole wood, cellulose, and lignin methoxyl groups.
26156050	2	44	theme	δ	254:254	arg1	C					259:259	δ(13)C	254:259	δ(13)C	254:259	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	0	45	theme	whole	24:28	arg1	groups					68:73	whole wood, α-cellulose and lignin methoxyl groups	24:73	whole wood, α-cellulose and lignin methoxyl groups	24:73	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	6	46	from	groups	952:957	arg1	H					936:936	δ(2)H	932:936	δ(2)H from methoxyl groups	932:957	On the other hand, δ(2)H from methoxyl groups showed high correlations with temperature and was also correlated with ring width, indicating its potential as a temperature proxy.
26156050	7	47	theme	group	1429:1433	arg1	H					1439:1439	(iv) methoxyl group δ(2)H	1415:1439	(iv) methoxyl group δ(2)H with spring temperatures	1415:1464	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	5	48	theme	climate	893:899	arg1	parameters					901:910	climate parameters	893:910	climate parameters	893:910	Compared with the whole wood samples, δ(13)C from methoxyl groups showed a different and overall lower response to climate parameters.
26156050	7	49	with	series	1104:1109	arg1	correlation					1128:1138	the highest correlation	1116:1138	the highest correlation with climatic parameter	1116:1162	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	50	theme	highest	1120:1126	arg1	correlation					1128:1138	the highest correlation	1116:1138	the highest correlation with climatic parameter	1116:1162	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	0	51	dep	δ²H	0:2	arg1	approach					114:121	a multi-parameter approach	96:121	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.	0:122	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	7	52	theme	annual	1366:1371	arg1	evapotranspiration					1373:1390	annual evapotranspiration	1366:1390	annual evapotranspiration	1366:1390	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	4	53	theme	lignin	754:759	arg1	groups					770:775	lignin methoxyl groups	754:775	lignin methoxyl groups	754:775	Results indicate significant correlations among the time series derived from whole wood, cellulose, and lignin methoxyl groups.
26156050	2	54	attach	derived	272:278	arg2	O					270:270	δ(18)O	265:270	δ(18)O derived from whole wood	265:294	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	2	54	attach	derived	272:278	arg1	wood					291:294	whole wood	285:294	whole wood	285:294	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	6	55	theme	other	920:924	arg1	hand					926:929	the other hand	916:929	the other hand	916:929	On the other hand, δ(2)H from methoxyl groups showed high correlations with temperature and was also correlated with ring width, indicating its potential as a temperature proxy.
26156050	3	56	theme	δ	502:502	arg1	O					507:507	δ(18)O	502:507	δ(18)O	502:507	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	7	57	with	correlation	1128:1138	arg1	parameter					1154:1162	climatic parameter	1145:1162	climatic parameter	1145:1162	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	58	theme	time	1099:1102	arg1	series					1104:1109	Isotope time series	1091:1109	Isotope time series with the highest correlation with climatic parameter	1091:1162	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	3	59	theme	whole	514:518	arg1	wood					520:523	whole wood	514:523	whole wood	514:523	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	1	60	theme	ring	135:138	arg1	H					168:168	δ(2)H	164:168	δ(2)H	164:168	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	60	theme	ring	135:138	arg1	parameters					140:149	Novel tree ring parameters	124:149	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups -	124:191	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	60	theme	ring	135:138	arg1	C					158:158	δ(13)C	153:158	δ(13)C	153:158	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	8	61	theme	wood	1587:1590	arg1	proxies					1592:1598	whole wood proxies	1581:1598	whole wood proxies	1581:1598	These findings reveal that multiple climate elements can be reconstructed from different wood components and that whole wood proxies perform comparably to cellulose time series.
26156050	5	62	theme	methoxyl	828:835	arg1	groups					837:842	methoxyl groups	828:842	methoxyl groups	828:842	Compared with the whole wood samples, δ(13)C from methoxyl groups showed a different and overall lower response to climate parameters.
26156050	1	63	from	groups	184:189	arg1	H					168:168	δ(2)H	164:168	δ(2)H	164:168	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	63	from	groups	184:189	arg1	parameters					140:149	Novel tree ring parameters	124:149	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups -	124:191	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	63	from	groups	184:189	arg1	C					158:158	δ(13)C	153:158	δ(13)C	153:158	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	6	64	with	correlations	971:982	arg1	temperature					989:999	temperature	989:999	temperature	989:999	On the other hand, δ(2)H from methoxyl groups showed high correlations with temperature and was also correlated with ring width, indicating its potential as a temperature proxy.
26156050	7	65	theme	summer	1244:1249	arg1	temperature					1251:1261	summer temperature	1244:1261	summer temperature	1244:1261	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	66	theme	cellulose	1333:1341	arg1	O					1348:1348	cellulose δ(18)O	1333:1348	cellulose δ(18)O	1333:1348	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	2	67	from	differences	338:348	arg1	composition					366:376	the isotopic composition	353:376	the isotopic composition	353:376	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	2	67	from	differences	338:348	arg1	signal					390:395	climate signal	382:395	climate signal	382:395	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	0	68	theme	multi-parameter	98:112	arg1	approach					114:121	a multi-parameter approach	96:121	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.	0:122	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	7	69	theme	growing	1211:1217	arg1	precipitation					1226:1238	growing season precipitation	1211:1238	growing season precipitation	1211:1238	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	3	70	theme	δ	548:548	arg1	C					553:553	(ii) δ(13)C	543:553	(ii) δ(13)C	543:553	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	3	71	dep	C	553:553	arg1	ii					544:545	ii	544:545	ii	544:545	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	7	72	with	wood	1180:1183	arg1	precipitation					1226:1238	growing season precipitation	1211:1238	growing season precipitation	1211:1238	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	72	with	wood	1180:1183	arg1	temperature					1251:1261	summer temperature	1244:1261	summer temperature	1244:1261	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	2	73	theme	wood	425:428	arg1	component					430:438	the extracted wood component	411:438	the extracted wood component	411:438	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	7	74	dep	wood	1180:1183	arg1	i					1171:1171	i	1171:1171	i	1171:1171	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	3	75	theme	methoxyl	570:577	arg1	groups					579:584	methoxyl groups	570:584	methoxyl groups	570:584	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	0	76	from	groups	68:73	arg1	δ¹³C					5:8	δ¹³C	5:8	δ¹³C	5:8	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	0	76	from	groups	68:73	arg1	δ²H					0:2	δ²H	0:2	δ²H	0:2	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	0	76	from	groups	68:73	arg1	δ¹⁸O					14:17	δ¹⁸O	14:17	δ¹⁸O	14:17	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	0	77	theme	α-cellulose	36:46	arg1	groups					68:73	whole wood, α-cellulose and lignin methoxyl groups	24:73	whole wood, α-cellulose and lignin methoxyl groups	24:73	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	3	78	theme	signal	456:461	arg1	dependency					463:472	this signal dependency	451:472	this signal dependency	451:472	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	2	79	with	samples	310:316	arg1	O					270:270	δ(18)O	265:270	δ(18)O derived from whole wood	265:294	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	2	79	with	samples	310:316	arg1	C					259:259	δ(13)C	254:259	δ(13)C	254:259	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	7	80	theme	whole	1318:1322	arg1	wood					1324:1327	(iii) whole wood	1312:1327	(iii) whole wood	1312:1327	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	81	theme	δ	1199:1199	arg1	C					1204:1204	cellulose δ(13)C	1189:1204	cellulose δ(13)C	1189:1204	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	0	82	theme	lignin	52:57	arg1	methoxyl					59:66	lignin methoxyl	52:66	lignin methoxyl	52:66	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	1	83	theme	Novel	124:128	arg1	H					168:168	δ(2)H	164:168	δ(2)H	164:168	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	83	theme	Novel	124:128	arg1	parameters					140:149	Novel tree ring parameters	124:149	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups -	124:191	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	83	theme	Novel	124:128	arg1	C					158:158	δ(13)C	153:158	δ(13)C	153:158	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	3	84	dep	H	563:563	arg1	2					561:561	2	561:561	2	561:561	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	0	85	dep	Pinus	78:82	arg1	sylvestris					84:93	Pinus sylvestris	78:93	Pinus sylvestris	78:93	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	7	86	theme	whole	1174:1178	arg1	wood					1180:1183	(i) whole wood	1170:1183	(i) whole wood	1170:1183	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	87	dep	wood	1324:1327	arg1	iii					1313:1315	iii	1313:1315	iii	1313:1315	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	3	88	theme	δ	559:559	arg1	H					563:563	δ(2)H	559:563	δ(2)H	559:563	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	5	89	theme	wood	802:805	arg1	samples					807:813	the whole wood samples	792:813	the whole wood samples	792:813	Compared with the whole wood samples, δ(13)C from methoxyl groups showed a different and overall lower response to climate parameters.
26156050	7	90	theme	spring	1446:1451	arg1	temperatures					1453:1464	spring temperatures	1446:1464	spring temperatures	1446:1464	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	91	theme	spring	1290:1295	arg1	precipitation					1297:1309	spring precipitation	1290:1309	spring precipitation	1290:1309	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	2	92	theme	isotopic	357:364	arg1	composition					366:376	the isotopic composition	353:376	the isotopic composition	353:376	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	8	93	theme	wood	1556:1559	arg1	components					1561:1570	different wood components	1546:1570	different wood components	1546:1570	These findings reveal that multiple climate elements can be reconstructed from different wood components and that whole wood proxies perform comparably to cellulose time series.
26156050	8	94	theme	cellulose	1622:1630	arg1	series					1637:1642	cellulose time series	1622:1642	cellulose time series	1622:1642	These findings reveal that multiple climate elements can be reconstructed from different wood components and that whole wood proxies perform comparably to cellulose time series.
26156050	6	95	theme	high	966:969	arg1	correlations					971:982	high correlations	966:982	high correlations with temperature	966:999	On the other hand, δ(2)H from methoxyl groups showed high correlations with temperature and was also correlated with ring width, indicating its potential as a temperature proxy.
26156050	3	96	dep	Pinus	593:597	arg1	L.					610:611	Pinus sylvestris L.	593:611	Pinus sylvestris L. growing near Altenkirchen (Germany)	593:647	We assess this signal dependency by analysing (i) δ(13)C and δ(18)O from whole wood and cellulose and (ii) δ(13)C and δ(2)H from methoxyl groups, using Pinus sylvestris L. growing near Altenkirchen (Germany).
26156050	2	97	theme	whole	285:289	arg1	wood					291:294	whole wood	285:294	whole wood	285:294	Tests with δ(13)C and δ(18)O derived from whole wood and cellulose samples, however, indicated differences in the isotopic composition and climate signal, depending on the extracted wood component.
26156050	8	98	theme	whole	1581:1585	arg1	proxies					1592:1598	whole wood proxies	1581:1598	whole wood proxies	1581:1598	These findings reveal that multiple climate elements can be reconstructed from different wood components and that whole wood proxies perform comparably to cellulose time series.
26156050	0	99	theme	wood	30:33	arg1	groups					68:73	whole wood, α-cellulose and lignin methoxyl groups	24:73	whole wood, α-cellulose and lignin methoxyl groups	24:73	δ²H, δ¹³C and δ¹⁸O from whole wood, α-cellulose and lignin methoxyl groups in Pinus sylvestris: a multi-parameter approach.
26156050	7	100	theme	methoxyl	1420:1427	arg1	H					1439:1439	(iv) methoxyl group δ(2)H	1415:1439	(iv) methoxyl group δ(2)H with spring temperatures	1415:1464	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	1	101	dep	parameters	140:149	arg1	H					168:168	δ(2)H	164:168	δ(2)H	164:168	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	101	dep	parameters	140:149	arg1	parameters					140:149	Novel tree ring parameters	124:149	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups -	124:191	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	101	dep	parameters	140:149	arg1	C					158:158	δ(13)C	153:158	δ(13)C	153:158	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	102	theme	δ	164:164	arg1	H					168:168	δ(2)H	164:168	δ(2)H	164:168	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	1	102	theme	δ	164:164	arg1	parameters					140:149	Novel tree ring parameters	124:149	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups -	124:191	Novel tree ring parameters - δ(13)C and δ(2)H from methoxyl groups - have been developed to reconstruct palaeoclimate.
26156050	7	103	theme	climatic	1145:1152	arg1	parameter					1154:1162	climatic parameter	1145:1162	climatic parameter	1145:1162	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
26156050	7	104	theme	water	1396:1400	arg1	balance					1402:1408	water balance	1396:1408	water balance	1396:1408	Isotope time series with the highest correlation with climatic parameter were: (i) whole wood and cellulose δ(13)C with growing season precipitation and summer temperature; (ii) methoxyl groups with spring precipitation; (iii) whole wood and cellulose δ(18)O correlates with annual evapotranspiration and water balance; and (iv) methoxyl group δ(2)H with spring temperatures.
29020512	3	0	theme	first	307:311	arg1	time					313:316	the first time	303:316	the first time	303:316	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	5	1	theme	Ulkenia	733:739	arg1	sp					741:742	Ulkenia sp	733:742	Ulkenia sp	733:742	RSM was performed on two objectives, that is, growth of Ulkenia sp.
29020512	7	2	theme	U/mL	1031:1034	arg1	value					1016:1020	the predicted value	1002:1020	the predicted value of 72.37 U/mL	1002:1034	When GA was conducted for maximization of the enzyme activity, the optimal α-amylase activity was found to be 71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL.
29020512	7	3	theme	activity	843:850	arg1	maximization					816:827	maximization	816:827	maximization of the enzyme activity	816:850	When GA was conducted for maximization of the enzyme activity, the optimal α-amylase activity was found to be 71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL.
29020512	3	4	from	protists	253:260	arg1	Enhancement					201:211	Enhancement	201:211	Enhancement of α-amylase production from the marine protists, thraustochytrids	201:278	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	3	4	from	protists	253:260	arg1	production					226:235	α-amylase production	216:235	α-amylase production from the marine protists, thraustochytrids	216:278	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	5	5	theme	sp	741:742	arg1	growth					723:728	growth	723:728	growth of Ulkenia sp	723:742	RSM was performed on two objectives, that is, growth of Ulkenia sp.
29020512	4	6	theme	factorial	504:512	arg1	design					545:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	3	7	theme	α-amylase	216:224	arg1	production					226:235	α-amylase production	216:235	α-amylase production from the marine protists, thraustochytrids	216:278	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	6	8	theme	α-amylase	770:778	arg1	activity					780:787	α-amylase activity	770:787	α-amylase activity	770:787	AH-2 (ATCC® PRA-296) and α-amylase activity.
29020512	8	9	theme	process	1069:1075	arg1	variables					1077:1085	the optimized process variables	1055:1085	the optimized process variables	1055:1085	Optimal growth at the optimized process variables was found to be 1.89A660nm.
29020512	4	10	theme	full	499:502	arg1	design					545:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	8	11	theme	optimized	1059:1067	arg1	variables					1077:1085	the optimized process variables	1055:1085	the optimized process variables	1055:1085	Optimal growth at the optimized process variables was found to be 1.89A660nm.
29020512	4	12	used	used	556:559	arg2	design					545:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	13	theme	interactive	585:595	arg1	effect					597:602	the cumulative interactive effect	570:602	the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract	570:674	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	3	14	theme	statistical-based	330:346	arg1	designs					361:367	statistical-based experimental designs	330:367	statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA)	330:435	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	3	15	theme	process	478:484	arg1	variables					486:494	the most influencing process variables	457:494	the most influencing process variables	457:494	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	7	16	theme	predicted	1006:1014	arg1	value					1016:1020	the predicted value	1002:1020	the predicted value of 72.37 U/mL	1002:1034	When GA was conducted for maximization of the enzyme activity, the optimal α-amylase activity was found to be 71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL.
29020512	8	17	theme	Optimal	1037:1043	arg1	growth					1045:1050	Optimal growth	1037:1050	Optimal growth at the optimized process variables	1037:1085	Optimal growth at the optimized process variables was found to be 1.89A660nm.
29020512	3	18	theme	experimental	348:359	arg1	designs					361:367	statistical-based experimental designs	330:367	statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA)	330:435	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	3	19	theme	marine	246:251	arg1	thraustochytrids					263:278	thraustochytrids	263:278	thraustochytrids	263:278	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	3	19	theme	marine	246:251	arg1	protists					253:260	the marine protists	242:260	the marine protists	242:260	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	0	20	theme	α-amylase	9:17	arg1	production					19:28	α-amylase production	9:28	α-amylase production	9:28	Enhanced α-amylase production by a marine protist, Ulkenia sp.
29020512	7	21	dep	agreement	987:995	arg1	both					965:968	both	965:968	both	965:968	When GA was conducted for maximization of the enzyme activity, the optimal α-amylase activity was found to be 71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL.
29020512	3	22	theme	production	226:235	arg1	Enhancement					201:211	Enhancement	201:211	Enhancement of α-amylase production from the marine protists, thraustochytrids	201:278	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	1	23	theme	response	69:76	arg1	methodology					86:96	response surface methodology	69:96	response surface methodology	69:96	using response surface methodology and genetic algorithm.
29020512	4	24	theme	yeast	662:666	arg1	extract					668:674	yeast extract	662:674	yeast extract	662:674	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	24	theme	yeast	662:666	arg1	components					619:628	nutritional components	607:628	nutritional components viz., glucose, corn starch, and yeast extract	607:674	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	9	25	theme	optimized	1119:1127	arg1	medium					1129:1134	The optimized medium	1115:1134	The optimized medium	1115:1134	The optimized medium increased α-amylase production by 1.2-fold.
29020512	1	26	theme	surface	78:84	arg1	methodology					86:96	response surface methodology	69:96	response surface methodology	69:96	using response surface methodology and genetic algorithm.
29020512	3	27	theme	response	375:382	arg1	methodology					392:402	response surface methodology	375:402	response surface methodology (RSM)	375:408	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	3	27	theme	response	375:382	arg1	RSM					405:407	RSM	405:407	RSM	405:407	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	3	28	theme	surface	384:390	arg1	methodology					392:402	response surface methodology	375:402	response surface methodology (RSM)	375:408	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	3	28	theme	surface	384:390	arg1	RSM					405:407	RSM	405:407	RSM	405:407	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	2	29	theme	wide	160:163	arg1	variety					165:171	a wide variety	158:171	a wide variety of industrial applications	158:198	Amylases are a group of enzymes with a wide variety of industrial applications.
29020512	2	29	theme	wide	160:163	arg1	applications					187:198	industrial applications	176:198	industrial applications	176:198	Amylases are a group of enzymes with a wide variety of industrial applications.
29020512	0	30	theme	marine	35:40	arg1	protist					42:48	a marine protist	33:48	a marine protist	33:48	Enhanced α-amylase production by a marine protist, Ulkenia sp.
29020512	0	30	theme	marine	35:40	arg1	sp					59:60	Ulkenia sp	51:60	Ulkenia sp	51:60	Enhanced α-amylase production by a marine protist, Ulkenia sp.
29020512	7	31	theme	which	973:977	arg1	agreement					987:995	agreement	987:995	agreement with the predicted value of 72.37 U/mL	987:1034	When GA was conducted for maximization of the enzyme activity, the optimal α-amylase activity was found to be 71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL.
29020512	9	32	theme	α-amylase	1146:1154	arg1	production					1156:1165	α-amylase production	1146:1165	α-amylase production	1146:1165	The optimized medium increased α-amylase production by 1.2-fold.
29020512	4	33	theme	corn	645:648	arg1	components					619:628	nutritional components	607:628	nutritional components viz., glucose, corn starch, and yeast extract	607:674	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	33	theme	corn	645:648	arg1	starch					650:655	corn starch	645:655	corn starch	645:655	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	3	34	theme	genetic	414:420	arg1	GA					433:434	GA	433:434	GA	433:434	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	3	34	theme	genetic	414:420	arg1	algorithm					422:430	genetic algorithm	414:430	genetic algorithm (GA)	414:435	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	4	35	theme	composite	522:530	arg1	design					545:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	2	36	with	group	136:140	arg1	variety					165:171	a wide variety	158:171	a wide variety of industrial applications	158:198	Amylases are a group of enzymes with a wide variety of industrial applications.
29020512	2	36	with	group	136:140	arg1	applications					187:198	industrial applications	176:198	industrial applications	176:198	Amylases are a group of enzymes with a wide variety of industrial applications.
29020512	3	37	from	Enhancement	201:211	arg1	thraustochytrids					263:278	thraustochytrids	263:278	thraustochytrids	263:278	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	3	37	from	Enhancement	201:211	arg1	protists					253:260	the marine protists	242:260	the marine protists	242:260	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	2	38	theme	enzymes	145:151	arg1	enzymes					145:151	enzymes	145:151	enzymes	145:151	Amylases are a group of enzymes with a wide variety of industrial applications.
29020512	2	38	theme	enzymes	145:151	arg1	Amylases					121:128	Amylases	121:128	Amylases	121:128	Amylases are a group of enzymes with a wide variety of industrial applications.
29020512	2	38	theme	enzymes	145:151	arg1	group					136:140	a group	134:140	a group of enzymes	134:151	Amylases are a group of enzymes with a wide variety of industrial applications.
29020512	1	39	theme	genetic	102:108	arg1	algorithm					110:118	genetic algorithm	102:118	genetic algorithm	102:118	using response surface methodology and genetic algorithm.
29020512	8	40	from	variables	1077:1085	arg1	growth					1045:1050	Optimal growth	1037:1050	Optimal growth at the optimized process variables	1037:1085	Optimal growth at the optimized process variables was found to be 1.89A660nm.
29020512	2	41	theme	applications	187:198	arg1	variety					165:171	a wide variety	158:171	a wide variety of industrial applications	158:198	Amylases are a group of enzymes with a wide variety of industrial applications.
29020512	2	41	theme	applications	187:198	arg1	applications					187:198	industrial applications	176:198	industrial applications	176:198	Amylases are a group of enzymes with a wide variety of industrial applications.
29020512	4	42	dep	components	619:628	arg1	extract					668:674	yeast extract	662:674	yeast extract	662:674	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	42	dep	components	619:628	arg1	components					619:628	nutritional components	607:628	nutritional components viz., glucose, corn starch, and yeast extract	607:674	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	42	dep	components	619:628	arg1	glucose					636:642	glucose	636:642	glucose	636:642	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	42	dep	components	619:628	arg1	starch					650:655	corn starch	645:655	corn starch	645:655	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	42	dep	components	619:628	arg1	viz.					630:633	viz.	630:633	viz.	630:633	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	3	43	theme	variables	486:494	arg1	optimization					441:452	optimization	441:452	optimization of the most influencing process variables	441:494	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	7	44	theme	optimal	857:863	arg1	71.20 U/mL					900:909	71.20 U/mL	900:909	71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL	900:1034	When GA was conducted for maximization of the enzyme activity, the optimal α-amylase activity was found to be 71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL.
29020512	7	44	theme	optimal	857:863	arg1	activity					875:882	the optimal α-amylase activity	853:882	the optimal α-amylase activity	853:882	When GA was conducted for maximization of the enzyme activity, the optimal α-amylase activity was found to be 71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL.
29020512	4	45	theme	experimental	532:543	arg1	design					545:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	2	46	theme	industrial	176:185	arg1	applications					187:198	industrial applications	176:198	industrial applications	176:198	Amylases are a group of enzymes with a wide variety of industrial applications.
29020512	3	47	theme	most	461:464	arg1	variables					486:494	the most influencing process variables	457:494	the most influencing process variables	457:494	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	7	48	theme	α-amylase	865:873	arg1	71.20 U/mL					900:909	71.20 U/mL	900:909	71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL	900:1034	When GA was conducted for maximization of the enzyme activity, the optimal α-amylase activity was found to be 71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL.
29020512	7	48	theme	α-amylase	865:873	arg1	activity					875:882	the optimal α-amylase activity	853:882	the optimal α-amylase activity	853:882	When GA was conducted for maximization of the enzyme activity, the optimal α-amylase activity was found to be 71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL.
29020512	4	49	theme	nutritional	607:617	arg1	extract					668:674	yeast extract	662:674	yeast extract	662:674	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	49	theme	nutritional	607:617	arg1	components					619:628	nutritional components	607:628	nutritional components viz., glucose, corn starch, and yeast extract	607:674	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	49	theme	nutritional	607:617	arg1	glucose					636:642	glucose	636:642	glucose	636:642	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	49	theme	nutritional	607:617	arg1	starch					650:655	corn starch	645:655	corn starch	645:655	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	49	theme	nutritional	607:617	arg1	viz.					630:633	viz.	630:633	viz.	630:633	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	4	50	theme	cumulative	574:583	arg1	effect					597:602	the cumulative interactive effect	570:602	the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract	570:674	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	3	51	theme	influencing	466:476	arg1	variables					486:494	the most influencing process variables	457:494	the most influencing process variables	457:494	Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical-based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables.
29020512	4	52	theme	central	514:520	arg1	design					545:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design	497:550	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
29020512	7	53	with	agreement	987:995	arg1	value					1016:1020	the predicted value	1002:1020	the predicted value of 72.37 U/mL	1002:1034	When GA was conducted for maximization of the enzyme activity, the optimal α-amylase activity was found to be 71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL.
29020512	0	54	theme	Ulkenia	51:57	arg1	protist					42:48	a marine protist	33:48	a marine protist	33:48	Enhanced α-amylase production by a marine protist, Ulkenia sp.
29020512	0	54	theme	Ulkenia	51:57	arg1	sp					59:60	Ulkenia sp	51:60	Ulkenia sp	51:60	Enhanced α-amylase production by a marine protist, Ulkenia sp.
29020512	7	55	theme	enzyme	836:841	arg1	activity					843:850	the enzyme activity	832:850	the enzyme activity	832:850	When GA was conducted for maximization of the enzyme activity, the optimal α-amylase activity was found to be 71.20 U/mL which was close to that obtained by RSM (71.93 U/mL), both of which were in agreement with the predicted value of 72.37 U/mL.
29020512	4	56	theme	components	619:628	arg1	effect					597:602	the cumulative interactive effect	570:602	the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract	570:674	A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz., glucose, corn starch, and yeast extract.
28285599	7	0	theme	precursor	1447:1455	arg1	trimethylamine					1467:1480	trimethylamine	1467:1480	trimethylamine (p < 0.05)	1467:1491	In addition, PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05), attenuated cholesterol accumulation, and nearly abolished atherogenesis in the model (p < 0.05).
28285599	7	0	theme	precursor	1447:1455	arg1	compound					1457:1464	the proatherogenic precursor compound	1428:1464	the proatherogenic precursor compound	1428:1464	In addition, PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05), attenuated cholesterol accumulation, and nearly abolished atherogenesis in the model (p < 0.05).
28285599	10	1	theme	systemic	2048:2055	arg1	health					2067:2072	systemic metabolic health	2048:2072	systemic metabolic health	2048:2072	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	3	2	theme	plant	724:728	arg1	ester					737:741	plant sterol ester	724:741	plant sterol ester (PSE)	724:747	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	2	theme	plant	724:728	arg1	PSE					744:746	PSE	744:746	PSE	744:746	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	9	3	theme	several	1821:1827	arg1	acylcarnitines					1829:1842	several acylcarnitines	1821:1842	several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05)	1821:1913	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	1	4	contain	have	177:180	arg1	microbiota					166:175	gut microbiota	162:175	gut microbiota	162:175	BACKGROUND There is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon.
28285599	1	4	contain	have	177:180	arg2	potential					186:194	the potential to modify	182:204	the potential to modify	182:204	BACKGROUND There is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon.
28285599	11	5	theme	host	2340:2343	arg1	effects					2345:2351	the direct host effects	2329:2351	the direct host effects	2329:2351	This study indicates that the gut microbiome-modifying effects of novel therapeutics should be considered, in addition to the direct host effects.
28285599	5	6	theme	few	1150:1152	arg1	alterations					1154:1164	relatively few alterations	1139:1164	relatively few alterations	1139:1164	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	8	7	theme	adiposity	1675:1683	arg1	degree					1665:1670	the greatest degree	1652:1670	the greatest degree of adiposity (p < 0.05)	1652:1694	However, PSE supplementation produced the heaviest mice with the greatest degree of adiposity (p < 0.05).
28285599	2	8	from	effects	349:355	arg1	microbiome					464:473	the gut microbiome	456:473	the gut microbiome	456:473	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	11	9	theme	microbiome-modifying	2241:2260	arg1	effects					2262:2268	the gut microbiome-modifying effects	2233:2268	the gut microbiome-modifying effects of novel therapeutics	2233:2290	This study indicates that the gut microbiome-modifying effects of novel therapeutics should be considered, in addition to the direct host effects.
28285599	10	10	contain	have	2132:2135	arg2	impact					2151:2156	a significant impact	2137:2156	a significant impact	2137:2156	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	10	10	contain	have	2132:2135	arg1	interventions					2114:2126	cardiovascular disease interventions	2091:2126	cardiovascular disease interventions	2091:2126	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	5	11	from	shifts	1003:1008	arg1	composition					1017:1027	the composition	1013:1027	the composition of the gut microbiome of PSE mice	1013:1061	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	5	12	dep	RESULTS	977:983	arg1	observed					988:995	observed	988:995	observed	988:995	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	5	13	theme	gut	1036:1038	arg1	microbiome					1040:1049	the gut microbiome	1032:1049	the gut microbiome of PSE mice	1032:1061	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	9	14	theme	metabolic	1882:1890	arg1	p < 0.05					1905:1912	p < 0.05	1905:1912	p < 0.05	1905:1912	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	9	14	theme	metabolic	1882:1890	arg1	dysfunction					1892:1902	metabolic dysfunction	1882:1902	metabolic dysfunction (p < 0.05)	1882:1913	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	3	15	theme	salt	778:781	arg1	2587					826:829	bile salt hydrolase-active Lactobacillus reuteri APC 2587	773:829	bile salt hydrolase-active Lactobacillus reuteri APC 2587	773:829	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	4	16	theme	gut	875:877	arg1	composition					890:900	The gut microbiome composition	871:900	The gut microbiome composition	871:900	The gut microbiome composition was then investigated, in addition to the host fecal and serum metabolome.
28285599	10	17	theme	significant	2139:2149	arg1	impact					2151:2156	a significant impact	2137:2156	a significant impact	2137:2156	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	1	18	theme	strong	130:135	arg1	evidence					137:144	strong evidence	130:144	strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon	130:281	BACKGROUND There is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon.
28285599	3	19	theme	drug	845:848	arg1	STAT					864:867	STAT	864:867	STAT	864:867	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	19	theme	drug	845:848	arg1	atorvastatin					850:861	the drug atorvastatin	841:861	the drug atorvastatin (STAT)	841:868	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	20	theme	APC	822:824	arg1	2587					826:829	bile salt hydrolase-active Lactobacillus reuteri APC 2587	773:829	bile salt hydrolase-active Lactobacillus reuteri APC 2587	773:829	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	10	21	theme	microbiome	2165:2174	arg1	composition					2176:2186	the microbiome composition	2161:2186	the microbiome composition	2161:2186	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	1	22	dep	BACKGROUND	110:119	arg1	is					127:128	is	127:128	is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon	127:281	BACKGROUND There is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon.
28285599	3	23	theme	Lactobacillus	800:812	arg1	2587					826:829	bile salt hydrolase-active Lactobacillus reuteri APC 2587	773:829	bile salt hydrolase-active Lactobacillus reuteri APC 2587	773:829	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	24	with	conjunction	659:669	arg1	interventions					697:709	three dietary interventions	683:709	three dietary interventions	683:709	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	24	with	conjunction	659:669	arg1	one					676:678	one	676:678	one	676:678	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	1	25	theme	gut	162:164	arg1	microbiota					166:175	gut microbiota	162:175	gut microbiota	162:175	BACKGROUND There is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon.
28285599	4	26	theme	serum	959:963	arg1	metabolome					965:974	the host fecal and serum metabolome	940:974	metabolome	965:974	The gut microbiome composition was then investigated, in addition to the host fecal and serum metabolome.
28285599	3	27	from	diet	606:609	arg1	conjunction					659:669	conjunction	659:669	conjunction with one of three dietary interventions	659:709	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	10	28	theme	host-derived	1980:1991	arg1	metabolites					1993:2003	microbial and host-derived metabolites	1966:2003	microbial and host-derived metabolites	1966:2003	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	5	29	theme	mice	1058:1061	arg1	microbiome					1040:1049	the gut microbiome	1032:1049	the gut microbiome of PSE mice	1032:1061	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	2	30	theme	neutraceutical	381:394	arg1	interventions					439:451	several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions	360:451	several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions	360:451	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	10	31	theme	microbial	1966:1974	arg1	metabolites					1993:2003	microbial and host-derived metabolites	1966:2003	microbial and host-derived metabolites	1966:2003	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	2	32	theme	interventions	439:451	arg1	effects					349:355	the compositional and functional effects	316:355	the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome	316:473	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	9	33	theme	considerable	1746:1757	arg1	impact					1759:1764	considerable impact	1746:1764	considerable impact	1746:1764	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	3	34	theme	interventions	697:709	arg1	interventions					697:709	three dietary interventions	683:709	three dietary interventions	683:709	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	34	theme	interventions	697:709	arg1	one					676:678	one	676:678	one	676:678	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	35	from	high-fat/cholesterol	635:654	arg1	conjunction					659:669	conjunction	659:669	conjunction with one of three dietary interventions	659:709	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	2	36	theme	cardiovascular	416:429	arg1	disease					431:437	pharmaceutical cardiovascular disease	401:437	pharmaceutical cardiovascular disease	401:437	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	5	37	theme	major	997:1001	arg1	shifts					1003:1008	major shifts	997:1008	major shifts in the composition of the gut microbiome of PSE mice	997:1061	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	0	38	theme	disease	70:76	arg1	metabolome					15:24	metabolome	15:24	metabolome	15:24	Microbiome and metabolome modifying effects of several cardiovascular disease interventions in apo-E-/- mice.
28285599	0	38	theme	disease	70:76	arg1	Microbiome					0:9	Microbiome	0:9	Microbiome	0:9	Microbiome and metabolome modifying effects of several cardiovascular disease interventions in apo-E-/- mice.
28285599	2	39	theme	functional	338:347	arg1	effects					349:355	the compositional and functional effects	316:355	the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome	316:473	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	0	40	theme	several	47:53	arg1	disease					70:76	several cardiovascular disease	47:76	several cardiovascular disease	47:76	Microbiome and metabolome modifying effects of several cardiovascular disease interventions in apo-E-/- mice.
28285599	6	41	from	reduction	1271:1279	arg1	isovalerate					1284:1294	isovalerate	1284:1294	isovalerate (p < 0.05)	1284:1305	Interestingly, these compositional effects imparted by PSE were coupled with an increase in acetate and reduction in isovalerate (p < 0.05), while OBG promoted n-butyrate synthesis (p < 0.01).
28285599	6	41	from	reduction	1271:1279	arg1	acetate					1259:1265	acetate	1259:1265	acetate	1259:1265	Interestingly, these compositional effects imparted by PSE were coupled with an increase in acetate and reduction in isovalerate (p < 0.05), while OBG promoted n-butyrate synthesis (p < 0.01).
28285599	6	41	from	reduction	1271:1279	arg1	p < 0.05					1297:1304	p < 0.05	1297:1304	p < 0.05	1297:1304	Interestingly, these compositional effects imparted by PSE were coupled with an increase in acetate and reduction in isovalerate (p < 0.05), while OBG promoted n-butyrate synthesis (p < 0.01).
28285599	9	42	theme	host	1773:1776	arg1	metabolome					1784:1793	the host serum metabolome	1769:1793	the host serum metabolome	1769:1793	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	2	43	theme	compositional	320:332	arg1	effects					349:355	the compositional and functional effects	316:355	the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome	316:473	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	8	44	theme	PSE	1600:1602	arg1	supplementation					1604:1618	PSE supplementation	1600:1618	PSE supplementation	1600:1618	However, PSE supplementation produced the heaviest mice with the greatest degree of adiposity (p < 0.05).
28285599	7	45	theme	compound	1457:1464	arg1	production					1414:1423	the microbial production	1400:1423	PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05),	1373:1492	In addition, PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05), attenuated cholesterol accumulation, and nearly abolished atherogenesis in the model (p < 0.05).
28285599	11	46	theme	therapeutics	2279:2290	arg1	effects					2262:2268	the gut microbiome-modifying effects	2233:2268	the gut microbiome-modifying effects of novel therapeutics	2233:2290	This study indicates that the gut microbiome-modifying effects of novel therapeutics should be considered, in addition to the direct host effects.
28285599	2	47	theme	nutritional	368:378	arg1	interventions					439:451	several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions	360:451	several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions	360:451	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	6	48	from	increase	1247:1254	arg1	isovalerate					1284:1294	isovalerate	1284:1294	isovalerate (p < 0.05)	1284:1305	Interestingly, these compositional effects imparted by PSE were coupled with an increase in acetate and reduction in isovalerate (p < 0.05), while OBG promoted n-butyrate synthesis (p < 0.01).
28285599	6	48	from	increase	1247:1254	arg1	acetate					1259:1265	acetate	1259:1265	acetate	1259:1265	Interestingly, these compositional effects imparted by PSE were coupled with an increase in acetate and reduction in isovalerate (p < 0.05), while OBG promoted n-butyrate synthesis (p < 0.01).
28285599	6	48	from	increase	1247:1254	arg1	p < 0.05					1297:1304	p < 0.05	1297:1304	p < 0.05	1297:1304	Interestingly, these compositional effects imparted by PSE were coupled with an increase in acetate and reduction in isovalerate (p < 0.05), while OBG promoted n-butyrate synthesis (p < 0.01).
28285599	7	49	theme	proatherogenic	1432:1445	arg1	trimethylamine					1467:1480	trimethylamine	1467:1480	trimethylamine (p < 0.05)	1467:1491	In addition, PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05), attenuated cholesterol accumulation, and nearly abolished atherogenesis in the model (p < 0.05).
28285599	7	49	theme	proatherogenic	1432:1445	arg1	compound					1457:1464	the proatherogenic precursor compound	1428:1464	the proatherogenic precursor compound	1428:1464	In addition, PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05), attenuated cholesterol accumulation, and nearly abolished atherogenesis in the model (p < 0.05).
28285599	0	50	dep	Microbiome	0:9	arg1	effects					36:42	modifying effects	26:42	modifying effects	26:42	Microbiome and metabolome modifying effects of several cardiovascular disease interventions in apo-E-/- mice.
28285599	10	51	theme	metabolic	2057:2065	arg1	health					2067:2072	systemic metabolic health	2048:2072	systemic metabolic health	2048:2072	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	6	52	theme	n-butyrate	1327:1336	arg1	p < 0.01					1349:1356	p < 0.01	1349:1356	p < 0.01	1349:1356	Interestingly, these compositional effects imparted by PSE were coupled with an increase in acetate and reduction in isovalerate (p < 0.05), while OBG promoted n-butyrate synthesis (p < 0.01).
28285599	6	52	theme	n-butyrate	1327:1336	arg1	synthesis					1338:1346	n-butyrate synthesis	1327:1346	n-butyrate synthesis (p < 0.01)	1327:1357	Interestingly, these compositional effects imparted by PSE were coupled with an increase in acetate and reduction in isovalerate (p < 0.05), while OBG promoted n-butyrate synthesis (p < 0.01).
28285599	8	53	theme	heaviest	1633:1640	arg1	mice					1642:1645	the heaviest mice	1629:1645	the heaviest mice	1629:1645	However, PSE supplementation produced the heaviest mice with the greatest degree of adiposity (p < 0.05).
28285599	1	54	theme	nutritional	239:249	arg1	interventions					251:263	nutritional interventions	239:263	nutritional interventions	239:263	BACKGROUND There is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon.
28285599	3	55	theme	sterol	730:735	arg1	ester					737:741	plant sterol ester	724:741	plant sterol ester (PSE)	724:747	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	55	theme	sterol	730:735	arg1	PSE					744:746	PSE	744:746	PSE	744:746	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	56	theme	high-fat/cholesterol	585:604	arg1	diet					606:609	high-fat/cholesterol diet	585:609	high-fat/cholesterol diet alone	585:615	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	7	57	theme	microbial	1404:1412	arg1	production					1414:1423	the microbial production	1400:1423	PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05),	1373:1492	In addition, PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05), attenuated cholesterol accumulation, and nearly abolished atherogenesis in the model (p < 0.05).
28285599	3	58	theme	oat	750:752	arg1	OBG					764:766	OBG	764:766	OBG	764:766	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	58	theme	oat	750:752	arg1	BSH					832:834	BSH	832:834	BSH	832:834	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	58	theme	oat	750:752	arg1	β-glucan					754:761	oat β-glucan	750:761	oat β-glucan (OBG)	750:767	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	8	59	theme	greatest	1656:1663	arg1	degree					1665:1670	the greatest degree	1652:1670	the greatest degree of adiposity (p < 0.05)	1652:1694	However, PSE supplementation produced the heaviest mice with the greatest degree of adiposity (p < 0.05).
28285599	7	60	theme	dampened	1391:1398	arg1	PSE					1373:1375	PSE	1373:1375	PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05),	1373:1492	In addition, PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05), attenuated cholesterol accumulation, and nearly abolished atherogenesis in the model (p < 0.05).
28285599	9	61	from	alterations	1806:1816	arg1	acylcarnitines					1829:1842	several acylcarnitines	1821:1842	several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05)	1821:1913	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	11	62	theme	gut	2237:2239	arg1	effects					2262:2268	the gut microbiome-modifying effects	2233:2268	the gut microbiome-modifying effects of novel therapeutics	2233:2290	This study indicates that the gut microbiome-modifying effects of novel therapeutics should be considered, in addition to the direct host effects.
28285599	1	63	dep	drugs	229:233	arg1	the					225:227	the	225:227	the	225:227	BACKGROUND There is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon.
28285599	10	64	theme	disease	2106:2112	arg1	interventions					2114:2126	cardiovascular disease interventions	2091:2126	cardiovascular disease interventions	2091:2126	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	5	65	theme	microbiome	1040:1049	arg1	composition					1017:1027	the composition	1013:1027	the composition of the gut microbiome of PSE mice	1013:1061	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	9	66	contain	have	1741:1744	arg1	PSE					1706:1708	PSE	1706:1708	PSE	1706:1708	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	9	66	contain	have	1741:1744	arg2	impact					1759:1764	considerable impact	1746:1764	considerable impact	1746:1764	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	9	66	contain	have	1741:1744	arg1	all					1725:1727	STAT all	1720:1727	STAT all	1720:1727	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	9	66	contain	have	1741:1744	arg1	OBG					1711:1713	OBG	1711:1713	OBG	1711:1713	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	10	67	theme	important	2021:2029	arg1	implications					2031:2042	important implications	2021:2042	important implications for systemic metabolic health	2021:2072	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	6	68	theme	compositional	1188:1200	arg1	effects					1202:1208	these compositional effects	1182:1208	these compositional effects imparted by PSE	1182:1224	Interestingly, these compositional effects imparted by PSE were coupled with an increase in acetate and reduction in isovalerate (p < 0.05), while OBG promoted n-butyrate synthesis (p < 0.01).
28285599	9	69	theme	dysfunction	1892:1902	arg1	state					1873:1877	a state	1871:1877	a state of metabolic dysfunction (p < 0.05)	1871:1913	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	3	70	theme	hydrolase-active	783:798	arg1	2587					826:829	bile salt hydrolase-active Lactobacillus reuteri APC 2587	773:829	bile salt hydrolase-active Lactobacillus reuteri APC 2587	773:829	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	5	71	theme	PSE	1054:1056	arg1	mice					1058:1061	PSE mice	1054:1061	PSE mice	1054:1061	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	3	72	dep	24 weeks	569:576	arg1	HFC					627:629	HFC	627:629	HFC	627:629	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	72	dep	24 weeks	569:576	arg1	control					618:624	control	618:624	control	618:624	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	72	dep	24 weeks	569:576	arg1	either					578:583	either	578:583	either	578:583	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	73	theme	bile	773:776	arg1	2587					826:829	bile salt hydrolase-active Lactobacillus reuteri APC 2587	773:829	bile salt hydrolase-active Lactobacillus reuteri APC 2587	773:829	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	4	74	dep	fecal	949:953	arg1	addition					928:935	addition	928:935	addition	928:935	The gut microbiome composition was then investigated, in addition to the host fecal and serum metabolome.
28285599	10	75	link	host-derived	1980:1991	arg1	metabolites					1993:2003	microbial and host-derived metabolites	1966:2003	microbial and host-derived metabolites	1966:2003	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	7	76	dep	PSE	1373:1375	arg1	production					1414:1423	the microbial production	1400:1423	PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05),	1373:1492	In addition, PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05), attenuated cholesterol accumulation, and nearly abolished atherogenesis in the model (p < 0.05).
28285599	4	77	theme	microbiome	879:888	arg1	composition					890:900	The gut microbiome composition	871:900	The gut microbiome composition	871:900	The gut microbiome composition was then investigated, in addition to the host fecal and serum metabolome.
28285599	4	78	theme	host	944:947	arg1	fecal					949:953	the host fecal and serum metabolome	940:974	fecal	949:953	The gut microbiome composition was then investigated, in addition to the host fecal and serum metabolome.
28285599	10	79	dep	CONCLUSIONS	1916:1926	arg1	observed					1931:1938	observed	1931:1938	observed	1931:1938	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	3	80	from	24 weeks	569:576	arg1	conjunction					659:669	conjunction	659:669	conjunction with one of three dietary interventions	659:709	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	10	81	theme	functional	1940:1949	arg1	alterations					1951:1961	functional alterations	1940:1961	functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health	1940:2072	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	0	82	theme	apo-E-/-	95:102	arg1	mice					104:107	apo-E-/- mice	95:107	apo-E-/- mice	95:107	Microbiome and metabolome modifying effects of several cardiovascular disease interventions in apo-E-/- mice.
28285599	5	83	theme	BSH	1078:1080	arg1	mice					1082:1085	OBG and BSH mice	1070:1085	OBG and BSH mice	1070:1085	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	2	84	theme	metabolomic	500:510	arg1	approaches					512:521	metagenomic and metabolomic approaches	484:521	metagenomic and metabolomic approaches	484:521	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	3	85	dep	Lactobacillus	800:812	arg1	reuteri					814:820	reuteri	814:820	reuteri	814:820	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	7	86	theme	cholesterol	1505:1515	arg1	accumulation					1517:1528	cholesterol accumulation	1505:1528	cholesterol accumulation	1505:1528	In addition, PSE significantly dampened the microbial production of the proatherogenic precursor compound, trimethylamine (p < 0.05), attenuated cholesterol accumulation, and nearly abolished atherogenesis in the model (p < 0.05).
28285599	11	87	theme	novel	2273:2277	arg1	therapeutics					2279:2290	novel therapeutics	2273:2290	novel therapeutics	2273:2290	This study indicates that the gut microbiome-modifying effects of novel therapeutics should be considered, in addition to the direct host effects.
28285599	9	88	theme	STAT	1720:1723	arg1	all					1725:1727	STAT all	1720:1727	STAT all	1720:1727	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	5	89	dep	more	1097:1100	arg1	fluctuations					1109:1120	modest fluctuations	1102:1120	modest fluctuations	1102:1120	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	5	90	theme	OBG	1070:1072	arg1	mice					1082:1085	OBG and BSH mice	1070:1085	OBG and BSH mice	1070:1085	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	2	91	theme	gut	460:462	arg1	microbiome					464:473	the gut microbiome	456:473	the gut microbiome	456:473	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	0	92	theme	modifying	26:34	arg1	effects					36:42	modifying effects	26:42	modifying effects	26:42	Microbiome and metabolome modifying effects of several cardiovascular disease interventions in apo-E-/- mice.
28285599	10	93	from	alterations	1951:1961	arg1	metabolites					1993:2003	microbial and host-derived metabolites	1966:2003	microbial and host-derived metabolites	1966:2003	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	3	94	dep	fed	561:563	arg1	atorvastatin					850:861	the drug atorvastatin	841:861	the drug atorvastatin (STAT)	841:868	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	94	dep	fed	561:563	arg1	ester					737:741	plant sterol ester	724:741	plant sterol ester (PSE)	724:747	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	94	dep	fed	561:563	arg1	BSH					832:834	BSH	832:834	BSH	832:834	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	94	dep	fed	561:563	arg1	β-glucan					754:761	oat β-glucan	750:761	oat β-glucan (OBG)	750:767	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	94	dep	fed	561:563	arg1	OBG					764:766	OBG	764:766	OBG	764:766	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	94	dep	fed	561:563	arg1	STAT					864:867	STAT	864:867	STAT	864:867	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	94	dep	fed	561:563	arg1	PSE					744:746	PSE	744:746	PSE	744:746	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	3	95	theme	dietary	689:695	arg1	interventions					697:709	three dietary interventions	683:709	three dietary interventions	683:709	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	10	96	theme	cardiovascular	2091:2104	arg1	interventions					2114:2126	cardiovascular disease interventions	2091:2126	cardiovascular disease interventions	2091:2126	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	2	97	theme	disease	431:437	arg1	interventions					439:451	several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions	360:451	several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions	360:451	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	2	98	theme	metagenomic	484:494	arg1	approaches					512:521	metagenomic and metabolomic approaches	484:521	metagenomic and metabolomic approaches	484:521	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	10	99	contain	have	2016:2019	arg1	metabolites					1993:2003	microbial and host-derived metabolites	1966:2003	microbial and host-derived metabolites	1966:2003	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	10	99	contain	have	2016:2019	arg2	implications					2031:2042	important implications	2021:2042	important implications for systemic metabolic health	2021:2072	CONCLUSIONS We observed functional alterations in microbial and host-derived metabolites, which may have important implications for systemic metabolic health, suggesting that cardiovascular disease interventions may have a significant impact on the microbiome composition and functionality.
28285599	2	100	theme	pharmaceutical	401:414	arg1	disease					431:437	pharmaceutical cardiovascular disease	401:437	pharmaceutical cardiovascular disease	401:437	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
28285599	0	101	theme	cardiovascular	55:68	arg1	disease					70:76	several cardiovascular disease	47:76	several cardiovascular disease	47:76	Microbiome and metabolome modifying effects of several cardiovascular disease interventions in apo-E-/- mice.
28285599	5	102	theme	modest	1102:1107	arg1	fluctuations					1109:1120	modest fluctuations	1102:1120	modest fluctuations	1102:1120	RESULTS We observed major shifts in the composition of the gut microbiome of PSE mice, while OBG and BSH mice displayed more modest fluctuations, and STAT showed relatively few alterations.
28285599	3	103	theme	Apolipoprotein-E-deficient	524:549	arg1	mice					551:554	Apolipoprotein-E-deficient mice	524:554	Apolipoprotein-E-deficient mice	524:554	Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT).
28285599	9	104	theme	serum	1778:1782	arg1	metabolome					1784:1793	the host serum metabolome	1769:1793	the host serum metabolome	1769:1793	Finally, PSE, OBG, and STAT all appeared to have considerable impact on the host serum metabolome, including alterations in several acylcarnitines previously associated with a state of metabolic dysfunction (p < 0.05).
28285599	11	105	theme	direct	2333:2338	arg1	effects					2345:2351	the direct host effects	2329:2351	the direct host effects	2329:2351	This study indicates that the gut microbiome-modifying effects of novel therapeutics should be considered, in addition to the direct host effects.
28285599	1	106	mod	modified	213:220	arg3	drugs					229:233	drugs	229:233	drugs	229:233	BACKGROUND There is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon.
28285599	1	106	mod	modified	213:220	arg1	microbiota					166:175	gut microbiota	162:175	gut microbiota	162:175	BACKGROUND There is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon.
28285599	1	106	mod	modified	213:220	arg3	interventions					251:263	nutritional interventions	239:263	nutritional interventions	239:263	BACKGROUND There is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon.
28285599	2	107	theme	several	360:366	arg1	interventions					439:451	several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions	360:451	several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions	360:451	This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches.
29658661	9	0	theme	bone	1262:1265	arg1	cement					1267:1272	the bone cement	1258:1272	the bone cement	1258:1272	The XRD pattern showed that the bone cement had a certain curability.
29658661	11	1	theme	MC3T3	1542:1546	arg1	cells					1548:1552	MC3T3 cells	1542:1552	MC3T3 cells	1542:1552	The immunofluorescence staining and MTT results indicated that MC3T3 cells grew better on CPC-CSNF-RGD after 240 min of culture(all <i>P</i><0.05).
29658661	5	2	theme	testing	810:816	arg1	instrument					818:827	universal mechanical testing instrument	789:827	universal mechanical testing instrument	789:827	The mechanical properties were measured by universal mechanical testing instrument.
29658661	1	3	theme	chitosan	253:260	arg1	CSNF					279:282	CSNF	279:282	CSNF	279:282	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	1	3	theme	chitosan	253:260	arg1	nanofibers					268:277	chitosan short nanofibers	253:277	chitosan short nanofibers(CSNF)	253:283	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	0	4	theme	RGD	88:90	arg1	peptide					92:98	RGD peptide	88:98	RGD peptide	88:98	[Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide grafted chitosan nanofibers].
29658661	9	5	contain	had	1274:1276	arg1	cement					1267:1272	the bone cement	1258:1272	the bone cement	1258:1272	The XRD pattern showed that the bone cement had a certain curability.
29658661	9	5	contain	had	1274:1276	arg2	curability					1288:1297	a certain curability	1278:1297	a certain curability	1278:1297	The XRD pattern showed that the bone cement had a certain curability.
29658661	1	6	theme	biomechanical	164:176	arg1	properties					196:205	the biomechanical and biocompatible properties	160:205	</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC)	143:239	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	1	7	theme	short	262:266	arg1	CSNF					279:282	CSNF	279:282	CSNF	279:282	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	1	7	theme	short	262:266	arg1	nanofibers					268:277	chitosan short nanofibers	253:277	chitosan short nanofibers(CSNF)	253:283	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	0	8	theme	chitosan	108:115	arg1	nanofibers					117:126	chitosan nanofibers	108:126	chitosan nanofibers	108:126	[Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide grafted chitosan nanofibers].
29658661	11	9	theme	MTT	1515:1517	arg1	results					1519:1525	The immunofluorescence staining and MTT results	1479:1525	results	1519:1525	The immunofluorescence staining and MTT results indicated that MC3T3 cells grew better on CPC-CSNF-RGD after 240 min of culture(all <i>P</i><0.05).
29658661	7	10	theme	porous	1022:1027	arg1	structure					1029:1037	the porous structure	1018:1037	the porous structure between the nanofibers	1018:1060	<b>Results:</b> The distribution of CSNF in the scaffold was homogeneous, and the porous structure between the nanofibers was observed by SEM.
29658661	4	11	theme	X-ray	686:690	arg1	XRD					705:707	XRD	705:707	XRD	705:707	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	11	theme	X-ray	686:690	arg1	spectrum					676:683	infrared spectrum	667:683	infrared spectrum	667:683	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	11	theme	X-ray	686:690	arg1	diffraction					692:702	X-ray diffraction	686:702	X-ray diffraction (XRD)	686:708	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	12	theme	infrared	667:674	arg1	spectrum					676:683	infrared spectrum	667:683	infrared spectrum	667:683	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	12	theme	infrared	667:674	arg1	diffraction					692:702	X-ray diffraction	686:702	X-ray diffraction (XRD)	686:708	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	13	theme	RGD	553:555	arg1	CSNF					566:569	RGD modified CSNF	553:569	RGD modified CSNF (CSNF-RGD)	553:580	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	13	theme	RGD	553:555	arg1	CPC					542:544	CPC	542:544	CPC	542:544	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	13	theme	RGD	553:555	arg1	CSNF-RGD					572:579	CSNF-RGD	572:579	CSNF-RGD	572:579	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	10	14	theme	break	1334:1338	arg1	strengths					1340:1348	break strengths	1334:1348	break strengths	1334:1348	The stain-stress test showed that break strengths were (17.74±0.54) MPa for CPC-CSNF and (16.67±0.56) MPa for CPCP-CSNF-RGD, both were higher than that of CPC(all <i>P</i><0.05).
29658661	12	15	theme	</b>	1641:1644	arg1	CSNF-RGD					1646:1653	</b> CSNF-RGD	1641:1653	</b> CSNF-RGD	1641:1653	<b>Conclusion:</b> CSNF-RGD can improve the biomechanical property and biocompatibility of CPC, indicating its potential application in bone tissue repair.
29658661	10	16	dep	MPa	1368:1370	arg1	both					1425:1428	both	1425:1428	both	1425:1428	The stain-stress test showed that break strengths were (17.74±0.54) MPa for CPC-CSNF and (16.67±0.56) MPa for CPCP-CSNF-RGD, both were higher than that of CPC(all <i>P</i><0.05).
29658661	6	17	dep	adhesion	834:841	arg1	The					830:832	The	830:832	The	830:832	The adhesion and proliferation of MC3T3 cells were assessed using immunofluorescence staining and MTT method.
29658661	4	18	theme	modified	617:624	arg1	CPC-CSNF-RGD					636:647	CPC-CSNF-RGD	636:647	CPC-CSNF-RGD	636:647	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	18	theme	modified	617:624	arg1	CPC-CSNF					626:633	RGD modified CPC-CSNF	613:633	RGD modified CPC-CSNF (CPC-CSNF-RGD)	613:648	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	18	theme	modified	617:624	arg1	CPC					542:544	CPC	542:544	CPC	542:544	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	3	19	theme	1.5:1	467:471	arg1	ratio					458:462	calcium phosphorus ratio	439:462	calcium phosphorus ratio of 1.5:1	439:471	CPC with calcium phosphorus ratio of 1.5:1 was prepared by Biocement D method.
29658661	8	20	theme	infrared	1087:1094	arg1	spectrum					1096:1103	The infrared spectrum	1083:1103	The infrared spectrum	1083:1103	The infrared spectrum showed the characteristic peaks at 1633 nm and 1585 nm, indicating that RGD was successfully grafted on chitosan nanofibers.
29658661	3	21	theme	phosphorus	447:456	arg1	ratio					458:462	calcium phosphorus ratio	439:462	calcium phosphorus ratio of 1.5:1	439:471	CPC with calcium phosphorus ratio of 1.5:1 was prepared by Biocement D method.
29658661	7	22	dep	<b>Results	940:949	arg1	observed					1066:1073	observed	1066:1073	was observed by SEM	1062:1080	<b>Results:</b> The distribution of CSNF in the scaffold was homogeneous, and the porous structure between the nanofibers was observed by SEM.
29658661	7	22	dep	<b>Results	940:949	arg1	homogeneous					1001:1011	homogeneous	1001:1011	homogeneous	1001:1011	<b>Results:</b> The distribution of CSNF in the scaffold was homogeneous, and the porous structure between the nanofibers was observed by SEM.
29658661	7	22	dep	<b>Results	940:949	arg1	distribution					960:971	The distribution	956:971	The distribution of CSNF in the scaffold	956:995	<b>Results:</b> The distribution of CSNF in the scaffold was homogeneous, and the porous structure between the nanofibers was observed by SEM.
29658661	12	23	theme	bone	1763:1766	arg1	repair					1775:1780	bone tissue repair	1763:1780	bone tissue repair	1763:1780	<b>Conclusion:</b> CSNF-RGD can improve the biomechanical property and biocompatibility of CPC, indicating its potential application in bone tissue repair.
29658661	7	24	from	distribution	960:971	arg1	scaffold					988:995	the scaffold	984:995	the scaffold	984:995	<b>Results:</b> The distribution of CSNF in the scaffold was homogeneous, and the porous structure between the nanofibers was observed by SEM.
29658661	1	25	theme	biocompatible	182:194	arg1	properties					196:205	the biomechanical and biocompatible properties	160:205	</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC)	143:239	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	9	26	theme	XRD	1234:1236	arg1	pattern					1238:1244	The XRD pattern	1230:1244	The XRD pattern	1230:1244	The XRD pattern showed that the bone cement had a certain curability.
29658661	4	27	theme	enhanced	588:595	arg1	CPC-CSNF					602:609	CPC-CSNF	602:609	CPC-CSNF	602:609	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	27	theme	enhanced	588:595	arg1	CPC					597:599	CSNF enhanced CPC	583:599	CSNF enhanced CPC (CPC-CSNF)	583:610	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	27	theme	enhanced	588:595	arg1	CPC					542:544	CPC	542:544	CPC	542:544	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	0	28	theme	[Biomechanical	0:13	arg1	enhancement					33:43	[Biomechanical and biocompatible enhancement	0:43	[Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide	0:98	[Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide grafted chitosan nanofibers].
29658661	6	29	theme	immunofluorescence	896:913	arg1	staining					915:922	immunofluorescence staining	896:922	immunofluorescence staining	896:922	The adhesion and proliferation of MC3T3 cells were assessed using immunofluorescence staining and MTT method.
29658661	12	30	theme	tissue	1768:1773	arg1	repair					1775:1780	bone tissue repair	1763:1780	bone tissue repair	1763:1780	<b>Conclusion:</b> CSNF-RGD can improve the biomechanical property and biocompatibility of CPC, indicating its potential application in bone tissue repair.
29658661	2	31	theme	speed	412:416	arg1	dispersion					418:427	high speed dispersion	407:427	high speed dispersion	407:427	<b>Methods:</b> Chitosan nanofibers were prepared by electrospinning, and cut into short fibers by high speed dispersion.
29658661	12	32	from	application	1748:1758	arg1	repair					1775:1780	bone tissue repair	1763:1780	bone tissue repair	1763:1780	<b>Conclusion:</b> CSNF-RGD can improve the biomechanical property and biocompatibility of CPC, indicating its potential application in bone tissue repair.
29658661	3	33	with	CPC	430:432	arg1	ratio					458:462	calcium phosphorus ratio	439:462	calcium phosphorus ratio of 1.5:1	439:471	CPC with calcium phosphorus ratio of 1.5:1 was prepared by Biocement D method.
29658661	8	34	theme	characteristic	1116:1129	arg1	peaks					1131:1135	the characteristic peaks	1112:1135	the characteristic peaks	1112:1135	The infrared spectrum showed the characteristic peaks at 1633 nm and 1585 nm, indicating that RGD was successfully grafted on chitosan nanofibers.
29658661	1	35	theme	calcium	210:216	arg1	CPC					236:238	CPC	236:238	CPC	236:238	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	1	35	theme	calcium	210:216	arg1	cement					228:233	calcium phosphate cement	210:233	calcium phosphate cement (CPC)	210:239	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	2	36	theme	high	407:410	arg1	dispersion					418:427	high speed dispersion	407:427	high speed dispersion	407:427	<b>Methods:</b> Chitosan nanofibers were prepared by electrospinning, and cut into short fibers by high speed dispersion.
29658661	4	37	theme	CPC	542:544	arg1	structure					529:537	structure	529:537	structure	529:537	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	37	theme	CPC	542:544	arg1	composition					513:523	composition	513:523	composition	513:523	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	1	38	dep	analysis	151:158	arg1	properties					196:205	the biomechanical and biocompatible properties	160:205	</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC)	143:239	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	1	38	dep	analysis	151:158	arg1	To					148:149	To	148:149	To	148:149	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	12	39	dep	property	1685:1692	arg1	the					1667:1669	the	1667:1669	the	1667:1669	<b>Conclusion:</b> CSNF-RGD can improve the biomechanical property and biocompatibility of CPC, indicating its potential application in bone tissue repair.
29658661	1	40	theme	phosphate	218:226	arg1	CPC					236:238	CPC	236:238	CPC	236:238	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	1	40	theme	phosphate	218:226	arg1	cement					228:233	calcium phosphate cement	210:233	calcium phosphate cement (CPC)	210:239	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	0	41	theme	reinforced	48:57	arg1	cement					77:82	reinforced calcium phosphate cement	48:82	reinforced calcium phosphate cement	48:82	[Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide grafted chitosan nanofibers].
29658661	1	42	theme	cement	228:233	arg1	properties					196:205	the biomechanical and biocompatible properties	160:205	</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC)	143:239	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	2	43	theme	short	391:395	arg1	fibers					397:402	short fibers	391:402	short fibers	391:402	<b>Methods:</b> Chitosan nanofibers were prepared by electrospinning, and cut into short fibers by high speed dispersion.
29658661	4	44	theme	RGD	613:615	arg1	CPC-CSNF-RGD					636:647	CPC-CSNF-RGD	636:647	CPC-CSNF-RGD	636:647	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	44	theme	RGD	613:615	arg1	CPC-CSNF					626:633	RGD modified CPC-CSNF	613:633	RGD modified CPC-CSNF (CPC-CSNF-RGD)	613:648	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	44	theme	RGD	613:615	arg1	CPC					542:544	CPC	542:544	CPC	542:544	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	6	45	theme	cells	870:874	arg1	proliferation					847:859	proliferation	847:859	proliferation	847:859	The adhesion and proliferation of MC3T3 cells were assessed using immunofluorescence staining and MTT method.
29658661	6	45	theme	cells	870:874	arg1	adhesion					834:841	adhesion	834:841	adhesion	834:841	The adhesion and proliferation of MC3T3 cells were assessed using immunofluorescence staining and MTT method.
29658661	6	46	theme	MTT	928:930	arg1	method					932:937	MTT method	928:937	MTT method	928:937	The adhesion and proliferation of MC3T3 cells were assessed using immunofluorescence staining and MTT method.
29658661	4	47	theme	electron	719:726	arg1	microscopy					728:737	electron microscopy	719:737	electron microscopy (SEM)	719:743	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	47	theme	electron	719:726	arg1	SEM					740:742	SEM	740:742	SEM	740:742	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	5	48	theme	mechanical	750:759	arg1	properties					761:770	The mechanical properties	746:770	The mechanical properties	746:770	The mechanical properties were measured by universal mechanical testing instrument.
29658661	4	49	theme	CSNF	583:586	arg1	CPC-CSNF					602:609	CPC-CSNF	602:609	CPC-CSNF	602:609	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	49	theme	CSNF	583:586	arg1	CPC					597:599	CSNF enhanced CPC	583:599	CSNF enhanced CPC (CPC-CSNF)	583:610	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	49	theme	CSNF	583:586	arg1	CPC					542:544	CPC	542:544	CPC	542:544	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	0	50	theme	phosphate	67:75	arg1	cement					77:82	reinforced calcium phosphate cement	48:82	reinforced calcium phosphate cement	48:82	[Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide grafted chitosan nanofibers].
29658661	5	51	theme	universal	789:797	arg1	instrument					818:827	universal mechanical testing instrument	789:827	universal mechanical testing instrument	789:827	The mechanical properties were measured by universal mechanical testing instrument.
29658661	12	52	theme	CPC	1718:1720	arg1	property					1685:1692	biomechanical property	1671:1692	biomechanical property	1671:1692	<b>Conclusion:</b> CSNF-RGD can improve the biomechanical property and biocompatibility of CPC, indicating its potential application in bone tissue repair.
29658661	12	52	theme	CPC	1718:1720	arg1	biocompatibility					1698:1713	biocompatibility	1698:1713	biocompatibility	1698:1713	<b>Conclusion:</b> CSNF-RGD can improve the biomechanical property and biocompatibility of CPC, indicating its potential application in bone tissue repair.
29658661	12	53	theme	biomechanical	1671:1683	arg1	property					1685:1692	biomechanical property	1671:1692	biomechanical property	1671:1692	<b>Conclusion:</b> CSNF-RGD can improve the biomechanical property and biocompatibility of CPC, indicating its potential application in bone tissue repair.
29658661	2	54	theme	Chitosan	324:331	arg1	nanofibers					333:342	</b> Chitosan nanofibers	319:342	</b> Chitosan nanofibers	319:342	<b>Methods:</b> Chitosan nanofibers were prepared by electrospinning, and cut into short fibers by high speed dispersion.
29658661	0	55	theme	calcium	59:65	arg1	cement					77:82	reinforced calcium phosphate cement	48:82	reinforced calcium phosphate cement	48:82	[Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide grafted chitosan nanofibers].
29658661	7	56	theme	CSNF	976:979	arg1	homogeneous					1001:1011	homogeneous	1001:1011	homogeneous	1001:1011	<b>Results:</b> The distribution of CSNF in the scaffold was homogeneous, and the porous structure between the nanofibers was observed by SEM.
29658661	7	56	theme	CSNF	976:979	arg1	distribution					960:971	The distribution	956:971	The distribution of CSNF in the scaffold	956:995	<b>Results:</b> The distribution of CSNF in the scaffold was homogeneous, and the porous structure between the nanofibers was observed by SEM.
29658661	2	57	theme	</b>	319:322	arg1	nanofibers					333:342	</b> Chitosan nanofibers	319:342	</b> Chitosan nanofibers	319:342	<b>Methods:</b> Chitosan nanofibers were prepared by electrospinning, and cut into short fibers by high speed dispersion.
29658661	10	58	theme	stain-stress	1304:1315	arg1	test					1317:1320	The stain-stress test	1300:1320	The stain-stress test	1300:1320	The stain-stress test showed that break strengths were (17.74±0.54) MPa for CPC-CSNF and (16.67±0.56) MPa for CPCP-CSNF-RGD, both were higher than that of CPC(all <i>P</i><0.05).
29658661	12	59	theme	potential	1738:1746	arg1	application					1748:1758	its potential application	1734:1758	its potential application in bone tissue repair	1734:1780	<b>Conclusion:</b> CSNF-RGD can improve the biomechanical property and biocompatibility of CPC, indicating its potential application in bone tissue repair.
29658661	11	60	theme	culture	1599:1605	arg1	min					1592:1594	240 min	1588:1594	240 min of culture(all <i>P</i><0.05)	1588:1624	The immunofluorescence staining and MTT results indicated that MC3T3 cells grew better on CPC-CSNF-RGD after 240 min of culture(all <i>P</i><0.05).
29658661	8	61	theme	chitosan	1209:1216	arg1	nanofibers					1218:1227	chitosan nanofibers	1209:1227	chitosan nanofibers	1209:1227	The infrared spectrum showed the characteristic peaks at 1633 nm and 1585 nm, indicating that RGD was successfully grafted on chitosan nanofibers.
29658661	9	62	theme	certain	1280:1286	arg1	curability					1288:1297	a certain curability	1278:1297	a certain curability	1278:1297	The XRD pattern showed that the bone cement had a certain curability.
29658661	11	63	theme	immunofluorescence	1483:1500	arg1	staining					1502:1509	The immunofluorescence staining and MTT results	1479:1525	staining	1502:1509	The immunofluorescence staining and MTT results indicated that MC3T3 cells grew better on CPC-CSNF-RGD after 240 min of culture(all <i>P</i><0.05).
29658661	1	64	theme	</b>	143:146	arg1	analysis					151:158	</b> To analysis	143:158	</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC)	143:239	<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD).
29658661	4	65	theme	modified	557:564	arg1	CSNF					566:569	RGD modified CSNF	553:569	RGD modified CSNF (CSNF-RGD)	553:580	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	65	theme	modified	557:564	arg1	CPC					542:544	CPC	542:544	CPC	542:544	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	4	65	theme	modified	557:564	arg1	CSNF-RGD					572:579	CSNF-RGD	572:579	CSNF-RGD	572:579	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	5	66	theme	mechanical	799:808	arg1	instrument					818:827	universal mechanical testing instrument	789:827	universal mechanical testing instrument	789:827	The mechanical properties were measured by universal mechanical testing instrument.
29658661	3	67	theme	calcium	439:445	arg1	ratio					458:462	calcium phosphorus ratio	439:462	calcium phosphorus ratio of 1.5:1	439:471	CPC with calcium phosphorus ratio of 1.5:1 was prepared by Biocement D method.
29658661	3	68	theme	Biocement	489:497	arg1	method					501:506	Biocement D method	489:506	Biocement D method	489:506	CPC with calcium phosphorus ratio of 1.5:1 was prepared by Biocement D method.
29658661	0	69	theme	cement	77:82	arg1	enhancement					33:43	[Biomechanical and biocompatible enhancement	0:43	[Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide	0:98	[Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide grafted chitosan nanofibers].
29658661	4	70	dep	composition	513:523	arg1	The					509:511	The	509:511	The	509:511	The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM).
29658661	0	71	theme	biocompatible	19:31	arg1	enhancement					33:43	[Biomechanical and biocompatible enhancement	0:43	[Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide	0:98	[Biomechanical and biocompatible enhancement of reinforced calcium phosphate cement via RGD peptide grafted chitosan nanofibers].
29658661	3	72	theme	D	499:499	arg1	method					501:506	Biocement D method	489:506	Biocement D method	489:506	CPC with calcium phosphorus ratio of 1.5:1 was prepared by Biocement D method.
29658661	6	73	theme	MC3T3	864:868	arg1	cells					870:874	MC3T3 cells	864:874	MC3T3 cells	864:874	The adhesion and proliferation of MC3T3 cells were assessed using immunofluorescence staining and MTT method.
26776058	4	0	theme	milk	812:815	arg1	banks					817:821	two non-profit milk banks	797:821	two non-profit milk banks	797:821	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	3	1	theme	milk	659:662	arg1	bank					664:667	a milk bank	657:667	a milk bank	657:667	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	3	2	theme	human	488:492	arg1	composition					499:509	human milk composition	488:509	human milk composition	488:509	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	6	3	theme	bank	1434:1437	arg1	samples					1439:1445	milk bank samples	1429:1445	milk bank samples	1429:1445	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	6	4	theme	A	1421:1421	arg1	concentrations					1472:1485	significantly lower concentrations	1452:1485	significantly lower concentrations of zinc, calcium, iron and oligosaccharides	1452:1529	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	6	4	theme	A	1421:1421	arg1	concentrations					1347:1360	significantly higher concentrations	1326:1360	significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples,	1326:1446	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	4	5	theme	pooled	841:846	arg1	samples					868:874	(N = 33) pooled, unpasteurized milk samples	832:874	(N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum	832:928	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	7	6	theme	milk	1648:1651	arg1	supply					1632:1637	the supply	1628:1637	the supply of donor milk	1628:1651	Accepting milk bank donations beyond one year postpartum is a potential strategy for increasing the supply of donor milk, but may require mineral fortification.
26776058	0	7	theme	milk	101:104	arg1	banking					106:112	human milk banking	95:112	human milk banking	95:112	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.
26776058	6	8	theme	zinc	1490:1493	arg1	concentrations					1472:1485	significantly lower concentrations	1452:1485	significantly lower concentrations of zinc, calcium, iron and oligosaccharides	1452:1529	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	6	8	theme	zinc	1490:1493	arg1	concentrations					1347:1360	significantly higher concentrations	1326:1360	significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples,	1326:1446	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	1	9	theme	first	187:191	arg1	year					193:196	the first year	183:196	the first year of lactation	183:209	While the composition of human milk has been studied extensively in the first year of lactation, there is a paucity of data regarding human milk composition beyond one year postpartum.
26776058	5	10	theme	calcium	1176:1182	arg1	concentrations					1184:1197	zinc and calcium concentrations	1167:1197	zinc and calcium concentrations	1167:1197	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	6	11	theme	lower	1466:1470	arg1	concentrations					1472:1485	significantly lower concentrations	1452:1485	significantly lower concentrations of zinc, calcium, iron and oligosaccharides	1452:1529	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	0	12	from	study	15:19	arg1	postpartum					66:75	the second year postpartum	50:75	the second year postpartum	50:75	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.
26776058	3	13	from	changes	477:483	arg1	composition					499:509	human milk composition	488:509	human milk composition	488:509	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	3	13	from	changes	477:483	arg1	year					525:528	the second year	514:528	the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank	514:667	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	0	14	theme	year	61:64	arg1	postpartum					66:75	the second year postpartum	50:75	the second year postpartum	50:75	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.
26776058	1	15	theme	human	140:144	arg1	milk					146:149	human milk	140:149	human milk	140:149	While the composition of human milk has been studied extensively in the first year of lactation, there is a paucity of data regarding human milk composition beyond one year postpartum.
26776058	5	16	theme	zinc	1167:1170	arg1	concentrations					1184:1197	zinc and calcium concentrations	1167:1197	zinc and calcium concentrations	1167:1197	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	5	17	theme	total	999:1003	arg1	protein					1005:1011	total protein	999:1011	total protein	999:1011	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	6	18	theme	oligosaccharides	1514:1529	arg1	concentrations					1472:1485	significantly lower concentrations	1452:1485	significantly lower concentrations of zinc, calcium, iron and oligosaccharides	1452:1529	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	6	18	theme	oligosaccharides	1514:1529	arg1	concentrations					1347:1360	significantly higher concentrations	1326:1360	significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples,	1326:1446	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	7	19	theme	postpartum	1578:1587	arg1	year					1573:1576	one year postpartum	1569:1587	one year postpartum	1569:1587	Accepting milk bank donations beyond one year postpartum is a potential strategy for increasing the supply of donor milk, but may require mineral fortification.
26776058	0	20	theme	milk	30:33	arg1	composition					35:45	human milk composition	24:45	human milk composition	24:45	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.
26776058	4	21	from	women	689:693	arg1	Carolina					704:711	Carolina	704:711	Carolina	704:711	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	2	22	theme	donate	396:401	arg1	milk					409:412	donate their milk	396:412	donate their milk	396:412	Policies vary at milk banks around the world regarding how long lactating women are eligible to donate their milk.
26776058	0	23	theme	second	54:59	arg1	postpartum					66:75	the second year postpartum	50:75	the second year postpartum	50:75	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.
26776058	5	24	theme	sodium	1076:1081	arg1	concentration					982:994	the concentration	978:994	the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium	978:1081	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	6	25	theme	calcium	1496:1502	arg1	concentrations					1472:1485	significantly lower concentrations	1452:1485	significantly lower concentrations of zinc, calcium, iron and oligosaccharides	1452:1529	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	6	25	theme	calcium	1496:1502	arg1	concentrations					1347:1360	significantly higher concentrations	1326:1360	significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples,	1326:1446	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	5	26	located	observed	1229:1236	arg1	potassium					1264:1272	potassium	1264:1272	potassium	1264:1272	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	5	26	located	observed	1229:1236	arg1	iron					1255:1258	iron	1255:1258	iron	1255:1258	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	5	26	located	observed	1229:1236	arg2	changes					1216:1222	no changes	1213:1222	no changes	1213:1222	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	5	26	located	observed	1229:1236	arg1	fat					1250:1252	fat	1250:1252	fat	1250:1252	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	5	26	located	observed	1229:1236	arg1	lactose					1241:1247	lactose	1241:1247	lactose	1241:1247	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	6	27	theme	year	1300:1303	arg1	postpartum					1305:1314	the second year postpartum	1289:1314	the second year postpartum	1289:1314	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	5	28	theme	longitudinal	1086:1097	arg1	samples					1099:1105	longitudinal samples	1086:1105	longitudinal samples of mother's milk	1086:1122	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	7	29	theme	milk	1542:1545	arg1	donations					1552:1560	milk bank donations	1542:1560	milk bank donations	1542:1560	Accepting milk bank donations beyond one year postpartum is a potential strategy for increasing the supply of donor milk, but may require mineral fortification.
26776058	2	30	theme	long	359:362	arg1	women					374:378	long lactating women	359:378	long lactating women	359:378	Policies vary at milk banks around the world regarding how long lactating women are eligible to donate their milk.
26776058	0	31	theme	human	24:28	arg1	composition					35:45	human milk composition	24:45	human milk composition	24:45	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.
26776058	1	32	theme	milk	255:258	arg1	composition					260:270	human milk composition	249:270	human milk composition	249:270	While the composition of human milk has been studied extensively in the first year of lactation, there is a paucity of data regarding human milk composition beyond one year postpartum.
26776058	5	33	theme	A	1052:1052	arg1	concentration					982:994	the concentration	978:994	the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium	978:1081	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	3	34	theme	based	580:584	arg1	guidelines					586:595	evidence based guidelines	571:595	evidence based guidelines	571:595	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	0	35	theme	composition	35:45	arg1	study					15:19	A longitudinal study	0:19	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.	0:113	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.
26776058	5	36	theme	oligosaccharides	1055:1070	arg1	concentration					982:994	the concentration	978:994	the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium	978:1081	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	5	37	theme	significant	943:953	arg1	P < 0.05					965:972	P < 0.05	965:972	P < 0.05	965:972	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	5	37	theme	significant	943:953	arg1	increase					955:962	a significant increase	941:962	a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk	941:1122	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	6	38	contain	contained	1316:1324	arg1	milk					1281:1284	Human milk	1275:1284	Human milk in the second year postpartum	1275:1314	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	6	38	contain	contained	1316:1324	arg2	concentrations					1472:1485	significantly lower concentrations	1452:1485	significantly lower concentrations of zinc, calcium, iron and oligosaccharides	1452:1529	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	6	38	contain	contained	1316:1324	arg2	concentrations					1347:1360	significantly higher concentrations	1326:1360	significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples,	1326:1446	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	4	39	theme	milk	730:733	arg1	samples					735:741	monthly milk samples	722:741	monthly milk samples from 11 months	722:756	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	3	40	theme	primary	419:425	arg1	purpose					427:433	The primary purpose	415:433	The primary purpose of this study	415:447	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	6	41	theme	lactoferrin	1380:1390	arg1	concentrations					1472:1485	significantly lower concentrations	1452:1485	significantly lower concentrations of zinc, calcium, iron and oligosaccharides	1452:1529	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	6	41	theme	lactoferrin	1380:1390	arg1	concentrations					1347:1360	significantly higher concentrations	1326:1360	significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples,	1326:1446	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	0	42	dep	study	15:19	arg1	implications					78:89	implications	78:89	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.	0:113	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.
26776058	3	43	theme	long	611:614	arg1	women					626:630	long lactating women	611:630	long lactating women	611:630	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	6	44	theme	protein	1371:1377	arg1	concentrations					1472:1485	significantly lower concentrations	1452:1485	significantly lower concentrations of zinc, calcium, iron and oligosaccharides	1452:1529	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	6	44	theme	protein	1371:1377	arg1	concentrations					1347:1360	significantly higher concentrations	1326:1360	significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples,	1326:1446	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	4	45	from	donors	893:898	arg1	samples					868:874	(N = 33) pooled, unpasteurized milk samples	832:874	(N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum	832:928	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	3	46	theme	study	443:447	arg1	purpose					427:433	The primary purpose	415:433	The primary purpose of this study	415:447	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	5	47	theme	lysozyme	1027:1034	arg1	concentration					982:994	the concentration	978:994	the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium	978:1081	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	7	48	theme	mineral	1670:1676	arg1	fortification					1678:1690	mineral fortification	1670:1690	mineral fortification	1670:1690	Accepting milk bank donations beyond one year postpartum is a potential strategy for increasing the supply of donor milk, but may require mineral fortification.
26776058	6	49	theme	higher	1340:1345	arg1	concentrations					1347:1360	significantly higher concentrations	1326:1360	significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples,	1326:1446	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	4	50	theme	non-profit	801:810	arg1	banks					817:821	two non-profit milk banks	797:821	two non-profit milk banks	797:821	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	3	51	theme	longitudinal	464:475	arg1	changes					477:483	longitudinal changes	464:483	longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank	464:667	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	6	52	theme	milk	1429:1432	arg1	samples					1439:1445	milk bank samples	1429:1445	milk bank samples	1429:1445	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	1	53	theme	milk	146:149	arg1	composition					125:135	the composition	121:135	the composition of human milk	121:149	While the composition of human milk has been studied extensively in the first year of lactation, there is a paucity of data regarding human milk composition beyond one year postpartum.
26776058	7	54	theme	donor	1642:1646	arg1	milk					1648:1651	donor milk	1642:1651	donor milk	1642:1651	Accepting milk bank donations beyond one year postpartum is a potential strategy for increasing the supply of donor milk, but may require mineral fortification.
26776058	3	55	theme	milk	494:497	arg1	composition					499:509	human milk composition	488:509	human milk composition	488:509	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	6	56	theme	Immunoglobulin	1406:1419	arg1	A					1421:1421	Immunoglobulin A	1406:1421	Immunoglobulin A	1406:1421	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	3	57	theme	postpartum	530:539	arg1	year					525:528	the second year	514:528	the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank	514:667	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	6	58	theme	lysozyme	1393:1400	arg1	concentrations					1472:1485	significantly lower concentrations	1452:1485	significantly lower concentrations of zinc, calcium, iron and oligosaccharides	1452:1529	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	6	58	theme	lysozyme	1393:1400	arg1	concentrations					1347:1360	significantly higher concentrations	1326:1360	significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples,	1326:1446	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	3	59	theme	second	518:523	arg1	year					525:528	the second year	514:528	the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank	514:667	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	6	60	from	milk	1281:1284	arg1	postpartum					1305:1314	the second year postpartum	1289:1314	the second year postpartum	1289:1314	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	4	61	from	11 months	748:756	arg1	samples					735:741	monthly milk samples	722:741	monthly milk samples from 11 months	722:756	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	6	62	theme	Human	1275:1279	arg1	milk					1281:1284	Human milk	1275:1284	Human milk in the second year postpartum	1275:1314	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	4	63	theme	approved	884:891	arg1	donors					893:898	51 approved donors	881:898	51 approved donors less than one year postpartum	881:928	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	5	64	from	increase	955:962	arg1	concentration					982:994	the concentration	978:994	the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium	978:1081	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	5	64	from	increase	955:962	arg1	samples					1099:1105	longitudinal samples	1086:1105	longitudinal samples of mother's milk	1086:1122	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	1	65	theme	postpartum	288:297	arg1	year					283:286	one year postpartum	279:297	one year postpartum	279:297	While the composition of human milk has been studied extensively in the first year of lactation, there is a paucity of data regarding human milk composition beyond one year postpartum.
26776058	4	66	theme	less	900:903	arg1	donors					893:898	51 approved donors	881:898	51 approved donors less than one year postpartum	881:928	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	5	67	theme	Immunoglobulin	1037:1050	arg1	A					1052:1052	Immunoglobulin A	1037:1052	Immunoglobulin A	1037:1052	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	7	68	theme	potential	1594:1602	arg1	strategy					1604:1611	a potential strategy	1592:1611	a potential strategy for increasing the supply of donor milk	1592:1651	Accepting milk bank donations beyond one year postpartum is a potential strategy for increasing the supply of donor milk, but may require mineral fortification.
26776058	4	69	theme	milk	863:866	arg1	samples					868:874	(N = 33) pooled, unpasteurized milk samples	832:874	(N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum	832:928	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	0	70	theme	human	95:99	arg1	banking					106:112	human milk banking	95:112	human milk banking	95:112	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.
26776058	5	71	theme	protein	1005:1011	arg1	concentration					982:994	the concentration	978:994	the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium	978:1081	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	4	72	dep	pooled	841:846	arg1	unpasteurized					849:861	unpasteurized	849:861	unpasteurized	849:861	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	0	73	theme	longitudinal	2:13	arg1	study					15:19	A longitudinal study	0:19	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.	0:113	A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking.
26776058	5	74	theme	lactoferrin	1014:1024	arg1	concentration					982:994	the concentration	978:994	the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium	978:1081	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
26776058	1	75	theme	data	234:237	arg1	paucity					223:229	a paucity	221:229	a paucity of data regarding human milk composition beyond one year postpartum	221:297	While the composition of human milk has been studied extensively in the first year of lactation, there is a paucity of data regarding human milk composition beyond one year postpartum.
26776058	3	76	theme	guidelines	586:595	arg1	development					556:566	the development	552:566	the development of evidence based guidelines	552:595	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	4	77	theme	lactating	679:687	arg1	women					689:693	Nineteen lactating women	670:693	Nineteen lactating women in North Carolina	670:711	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	2	78	theme	lactating	364:372	arg1	women					374:378	long lactating women	359:378	long lactating women	359:378	Policies vary at milk banks around the world regarding how long lactating women are eligible to donate their milk.
26776058	6	79	theme	second	1293:1298	arg1	year					1300:1303	the second year	1289:1303	the second year postpartum	1289:1314	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	1	80	theme	human	249:253	arg1	composition					260:270	human milk composition	249:270	human milk composition	249:270	While the composition of human milk has been studied extensively in the first year of lactation, there is a paucity of data regarding human milk composition beyond one year postpartum.
26776058	7	81	theme	bank	1547:1550	arg1	donations					1552:1560	milk bank donations	1542:1560	milk bank donations	1542:1560	Accepting milk bank donations beyond one year postpartum is a potential strategy for increasing the supply of donor milk, but may require mineral fortification.
26776058	1	82	theme	lactation	201:209	arg1	year					193:196	the first year	183:196	the first year of lactation	183:209	While the composition of human milk has been studied extensively in the first year of lactation, there is a paucity of data regarding human milk composition beyond one year postpartum.
26776058	4	83	theme	postpartum	919:928	arg1	year					914:917	one year postpartum	910:928	one year postpartum	910:928	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	3	84	theme	lactating	616:624	arg1	women					626:630	long lactating women	611:630	long lactating women	611:630	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	4	85	theme	17 months	761:769	arg1	N = 131					783:789	N = 131	783:789	N = 131	783:789	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	4	85	theme	17 months	761:769	arg1	postpartum					771:780	17 months postpartum	761:780	17 months postpartum (N = 131)	761:790	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	6	86	theme	iron	1505:1508	arg1	concentrations					1472:1485	significantly lower concentrations	1452:1485	significantly lower concentrations of zinc, calcium, iron and oligosaccharides	1452:1529	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	6	86	theme	iron	1505:1508	arg1	concentrations					1347:1360	significantly higher concentrations	1326:1360	significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples,	1326:1446	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	4	87	theme	monthly	722:728	arg1	samples					735:741	monthly milk samples	722:741	monthly milk samples from 11 months	722:756	Nineteen lactating women in North Carolina provided monthly milk samples from 11 months to 17 months postpartum (N = 131), and two non-profit milk banks provided (N = 33) pooled, unpasteurized milk samples from 51 approved donors less than one year postpartum.
26776058	3	88	dep	based	580:584	arg1	evidence					571:578	evidence	571:578	evidence	571:578	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	3	89	theme	human	643:647	arg1	milk					649:652	human milk	643:652	human milk	643:652	The primary purpose of this study is to describe longitudinal changes in human milk composition in the second year postpartum to support the development of evidence based guidelines regarding how long lactating women can donate human milk to a milk bank.
26776058	2	90	theme	milk	317:320	arg1	banks					322:326	milk banks	317:326	milk banks around the world	317:343	Policies vary at milk banks around the world regarding how long lactating women are eligible to donate their milk.
26776058	6	91	theme	total	1365:1369	arg1	protein					1371:1377	total protein	1365:1377	total protein	1365:1377	Human milk in the second year postpartum contained significantly higher concentrations of total protein, lactoferrin, lysozyme and Immunoglobulin A, than milk bank samples, and significantly lower concentrations of zinc, calcium, iron and oligosaccharides.
26776058	5	92	theme	17 months	1139:1147	arg1	postpartum					1149:1158	17 months postpartum	1139:1158	17 months postpartum	1139:1158	There was a significant increase (P < 0.05) in the concentration of total protein, lactoferrin, lysozyme, Immunoglobulin A, oligosaccharides and sodium in longitudinal samples of mother's milk between 11 and 17 months postpartum, while zinc and calcium concentrations declined, and no changes were observed in lactose, fat, iron and potassium.
25504184	8	0	theme	pores	1544:1548	arg1	surfaces					1477:1484	the surfaces	1473:1484	the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores	1473:1548	LbL-chitosan promoted punctate mineral deposition on the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores.
25504184	3	1	theme	-deposited	650:659	arg1	polyelectrolytes					661:676	Layer-by-Layer (LbL)-deposited polyelectrolytes	630:676	Layer-by-Layer (LbL)-deposited polyelectrolytes	630:676	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	2	2	theme	geometry	336:343	arg1	role					319:322	the role	315:322	the role of internal geometry and cell-surface attachment on osseous integration potential	315:404	Limited information is available on the role of internal geometry and cell-surface attachment on osseous integration potential.
25504184	4	3	theme	discs	838:842	arg1	depths					815:820	all depths	811:820	all depths of the scaffold discs	811:842	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	6	4	theme	fibroblast-like	1157:1171	arg1	cells					1173:1177	fibroblast-like cells	1157:1177	fibroblast-like cells	1157:1177	Most pores, with or without chitosan, became filled by collagen networks sparsely populated with fibroblast-like cells.
25504184	1	5	theme	tissue	246:251	arg1	applications					265:276	bone tissue engineering applications	241:276	bone tissue engineering applications	241:276	Poly(epsilon-caprolactone) (PCL) is a hydrophobic bioplastic under development for bone tissue engineering applications.
25504184	9	6	from	role	1731:1734	arg1	differentiation					1787:1801	osteoblast terminal differentiation	1767:1801	osteoblast terminal differentiation	1767:1801	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	3	7	theme	PCL	523:525	arg1	scaffolds					527:535	porous 3D PCL scaffolds	513:535	porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes	513:676	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	7	8	theme	µm	1395:1396	arg1	pores					1398:1402	141 µm pores	1391:1402	141 µm pores near the edges	1391:1417	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	7	9	from	medium	1219:1224	arg1	days					1189:1192	21 days	1186:1192	21 days of culture in osteogenic medium	1186:1224	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	0	10	theme	3D	110:111	arg1	scaffolds					147:155	3D porous poly(epsilon-caprolactone) scaffolds	110:155	3D porous poly(epsilon-caprolactone) scaffolds	110:155	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	3	11	theme	interconnected	548:561	arg1	pores					576:580	fully interconnected 84 or 141 µm pores	542:580	fully interconnected 84 or 141 µm pores	542:580	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	0	12	theme	poly	120:123	arg1	scaffolds					147:155	3D porous poly(epsilon-caprolactone) scaffolds	110:155	3D porous poly(epsilon-caprolactone) scaffolds	110:155	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	7	13	theme	cellular	1312:1319	arg1	layers					1329:1334	highly cellular surface layers	1305:1334	highly cellular surface layers that enveloped all scaffolds	1305:1363	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	7	14	located	detected	1262:1269	arg2	mineralization					1243:1256	sporadic matrix mineralization	1227:1256	sporadic matrix mineralization	1227:1256	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	7	14	located	detected	1262:1269	arg1	layers					1329:1334	highly cellular surface layers	1305:1334	highly cellular surface layers that enveloped all scaffolds	1305:1363	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	9	15	theme	mineral	1663:1669	arg1	formation					1671:1679	mineral formation	1663:1679	mineral formation in 84 µm pores	1663:1694	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	4	16	from	medium	893:898	arg1	days					918:921	21 additional days	904:921	21 additional days in osteogenic medium	904:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	4	16	from	medium	893:898	arg1	days					860:863	10 days	857:863	10 days of culture in proliferation medium	857:898	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	4	17	theme	osteogenic	926:935	arg1	medium					937:942	osteogenic medium	926:942	osteogenic medium	926:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	0	18	theme	in	67:68	arg1	fibrosis					76:83	in vitro fibrosis	67:83	in vitro fibrosis	67:83	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	4	19	theme	scaffold	829:836	arg1	discs					838:842	the scaffold discs	825:842	the scaffold discs	825:842	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	7	20	from	aggregates	1377:1386	arg1	pores					1398:1402	141 µm pores	1391:1402	141 µm pores near the edges	1391:1417	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	9	21	theme	µm	1687:1688	arg1	pores					1690:1694	84 µm pores	1684:1694	84 µm pores	1684:1694	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	8	22	theme	mineral	1451:1457	arg1	deposition					1459:1468	punctate mineral deposition	1442:1468	punctate mineral deposition on the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores	1442:1548	LbL-chitosan promoted punctate mineral deposition on the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores.
25504184	4	23	theme	static	750:755	arg1	method					770:775	a novel static cold seeding method	742:775	a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium	742:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	3	24	theme	human	437:441	arg1	marrow					448:453	human bone marrow	437:453	human bone marrow mesenchymal stem cells (MSCs)	437:483	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	5	25	theme	pore	1045:1048	arg1	surfaces					1050:1057	chitosan-coated pore surfaces	1029:1057	chitosan-coated pore surfaces	1029:1057	MSCs were observed by SEM and histology to spread faster and to proliferate more on chitosan-coated pore surfaces.
25504184	4	26	theme	PCL	719:721	arg1	cylinders					726:734	PCL 3D cylinders	719:734	PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium	719:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	2	27	theme	osseous	376:382	arg1	potential					396:404	osseous integration potential	376:404	osseous integration potential	376:404	Limited information is available on the role of internal geometry and cell-surface attachment on osseous integration potential.
25504184	0	28	theme	Pore	0:3	arg1	size					5:8	Pore size	0:8	Pore size	0:8	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	1	29	theme	engineering	253:263	arg1	applications					265:276	bone tissue engineering applications	241:276	bone tissue engineering applications	241:276	Poly(epsilon-caprolactone) (PCL) is a hydrophobic bioplastic under development for bone tissue engineering applications.
25504184	0	30	dep	in	67:68	arg1	vitro					70:74	vitro	70:74	vitro	70:74	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	9	31	theme	inhibitory	1720:1729	arg1	role					1731:1734	a potential inhibitory role	1708:1734	a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation	1708:1801	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	3	32	theme	stem	467:470	arg1	MSCs					479:482	MSCs	479:482	MSCs	479:482	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	3	32	theme	stem	467:470	arg1	cells					472:476	human bone marrow mesenchymal stem cells	437:476	human bone marrow mesenchymal stem cells (MSCs)	437:483	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	8	33	theme	pores	1495:1499	arg1	surfaces					1477:1484	the surfaces	1473:1484	the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores	1473:1548	LbL-chitosan promoted punctate mineral deposition on the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores.
25504184	4	34	theme	%	781:781	arg1	serum					783:787	2% serum	780:787	2% serum	780:787	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	3	35	theme	marrow	448:453	arg1	MSCs					479:482	MSCs	479:482	MSCs	479:482	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	3	35	theme	marrow	448:453	arg1	cells					472:476	human bone marrow mesenchymal stem cells	437:476	human bone marrow mesenchymal stem cells (MSCs)	437:483	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	7	36	theme	sporadic	1227:1234	arg1	mineralization					1243:1256	sporadic matrix mineralization	1227:1256	sporadic matrix mineralization	1227:1256	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	0	37	theme	stem	57:60	arg1	cell					62:65	mesenchymal stem cell in vitro fibrosis and biomineralization	45:105	cell	62:65	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	2	38	theme	internal	327:334	arg1	geometry					336:343	internal geometry	327:343	internal geometry	327:343	Limited information is available on the role of internal geometry and cell-surface attachment on osseous integration potential.
25504184	9	39	theme	osteoblast	1767:1776	arg1	differentiation					1787:1801	osteoblast terminal differentiation	1767:1801	osteoblast terminal differentiation	1767:1801	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	4	40	theme	seeding	762:768	arg1	method					770:775	a novel static cold seeding method	742:775	a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium	742:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	2	41	theme	attachment	362:371	arg1	role					319:322	the role	315:322	the role of internal geometry and cell-surface attachment on osseous integration potential	315:404	Limited information is available on the role of internal geometry and cell-surface attachment on osseous integration potential.
25504184	4	42	from	days	918:921	arg1	medium					937:942	osteogenic medium	926:942	osteogenic medium	926:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	4	42	from	days	918:921	arg1	medium					893:898	proliferation medium	879:898	proliferation medium	879:898	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	0	43	theme	mesenchymal	45:55	arg1	cell					62:65	mesenchymal stem cell in vitro fibrosis and biomineralization	45:105	cell	62:65	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	2	44	from	role	319:322	arg1	potential					396:404	osseous integration potential	376:404	osseous integration potential	376:404	Limited information is available on the role of internal geometry and cell-surface attachment on osseous integration potential.
25504184	8	45	theme	µm	1541:1542	arg1	pores					1544:1548	the 141 µm pores	1533:1548	84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores	1489:1548	LbL-chitosan promoted punctate mineral deposition on the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores.
25504184	2	46	theme	Limited	279:285	arg1	information					287:297	Limited information	279:297	Limited information	279:297	Limited information is available on the role of internal geometry and cell-surface attachment on osseous integration potential.
25504184	1	47	theme	bone	241:244	arg1	applications					265:276	bone tissue engineering applications	241:276	bone tissue engineering applications	241:276	Poly(epsilon-caprolactone) (PCL) is a hydrophobic bioplastic under development for bone tissue engineering applications.
25504184	9	48	theme	LbL-chitosan	1576:1587	arg1	coatings					1589:1596	LbL-chitosan coatings	1576:1596	LbL-chitosan coatings	1576:1596	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	7	49	from	days	1189:1192	arg1	medium					1219:1224	osteogenic medium	1208:1224	osteogenic medium	1208:1224	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	3	50	theme	3D	520:521	arg1	scaffolds					527:535	porous 3D PCL scaffolds	513:535	porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes	513:676	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	7	51	from	culture	1197:1203	arg1	medium					1219:1224	osteogenic medium	1208:1224	osteogenic medium	1208:1224	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	7	52	theme	cell	1372:1375	arg1	aggregates					1377:1386	cell aggregates	1372:1386	cell aggregates in 141 µm pores near the edges	1372:1417	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	0	53	theme	porous	113:118	arg1	poly					120:123	porous poly	113:123	3D porous poly(epsilon-caprolactone) scaffolds	110:155	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	0	53	theme	porous	113:118	arg1	epsilon-caprolactone					125:144	epsilon-caprolactone	125:144	epsilon-caprolactone	125:144	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	9	54	theme	MSC	1624:1626	arg1	attachment					1628:1637	MSC attachment	1624:1637	MSC attachment to PCL	1624:1644	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	4	55	from	culture	868:874	arg1	medium					893:898	proliferation medium	879:898	proliferation medium	879:898	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	4	56	theme	culture	868:874	arg1	days					918:921	21 additional days	904:921	21 additional days in osteogenic medium	904:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	4	56	theme	culture	868:874	arg1	days					860:863	10 days	857:863	10 days of culture in proliferation medium	857:898	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	4	57	from	days	860:863	arg1	medium					937:942	osteogenic medium	926:942	osteogenic medium	926:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	4	57	from	days	860:863	arg1	medium					893:898	proliferation medium	879:898	proliferation medium	879:898	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	9	58	link	MSC-derived	1740:1750	arg1	fibroblasts					1752:1762	MSC-derived fibroblasts	1740:1762	MSC-derived fibroblasts	1740:1762	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	4	59	theme	trypsinized	687:697	arg1	MSCs					699:702	Freshly trypsinized MSCs	679:702	Freshly trypsinized MSCs	679:702	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	8	60	dep	pores	1495:1499	arg1	PCL-only					1515:1522	PCL-only	1515:1522	PCL-only	1515:1522	LbL-chitosan promoted punctate mineral deposition on the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores.
25504184	8	60	dep	pores	1495:1499	arg1	p < 0.05					1502:1509	p < 0.05	1502:1509	p < 0.05	1502:1509	LbL-chitosan promoted punctate mineral deposition on the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores.
25504184	7	61	theme	culture	1197:1203	arg1	days					1189:1192	21 days	1186:1192	21 days of culture in osteogenic medium	1186:1224	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	4	62	from	method	770:775	arg1	serum					783:787	2% serum	780:787	2% serum	780:787	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	4	63	theme	additional	907:916	arg1	days					918:921	21 additional days	904:921	21 additional days in osteogenic medium	904:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	8	64	theme	punctate	1442:1449	arg1	deposition					1459:1468	punctate mineral deposition	1442:1468	punctate mineral deposition on the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores	1442:1548	LbL-chitosan promoted punctate mineral deposition on the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores.
25504184	6	65	theme	collagen	1115:1122	arg1	networks					1124:1131	collagen networks	1115:1131	collagen networks sparsely populated with fibroblast-like cells	1115:1177	Most pores, with or without chitosan, became filled by collagen networks sparsely populated with fibroblast-like cells.
25504184	4	66	theme	proliferation	879:891	arg1	medium					893:898	proliferation medium	879:898	proliferation medium	879:898	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	5	67	theme	chitosan-coated	1029:1043	arg1	surfaces					1050:1057	chitosan-coated pore surfaces	1029:1057	chitosan-coated pore surfaces	1029:1057	MSCs were observed by SEM and histology to spread faster and to proliferate more on chitosan-coated pore surfaces.
25504184	4	68	theme	novel	744:748	arg1	method					770:775	a novel static cold seeding method	742:775	a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium	742:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	2	69	theme	integration	384:394	arg1	potential					396:404	osseous integration potential	376:404	osseous integration potential	376:404	Limited information is available on the role of internal geometry and cell-surface attachment on osseous integration potential.
25504184	8	70	from	deposition	1459:1468	arg1	surfaces					1477:1484	the surfaces	1473:1484	the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores	1473:1548	LbL-chitosan promoted punctate mineral deposition on the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores.
25504184	9	71	theme	potential	1710:1718	arg1	role					1731:1734	a potential inhibitory role	1708:1734	a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation	1708:1801	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	8	72	theme	µm	1492:1493	arg1	pores					1495:1499	84 µm pores	1489:1499	84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores	1489:1548	LbL-chitosan promoted punctate mineral deposition on the surfaces of 84 µm pores (p < 0.05 vs. PCL-only) but not the 141 µm pores.
25504184	0	73	theme	LbL	14:16	arg1	chitosan					18:25	LbL chitosan	14:25	LbL chitosan	14:25	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	0	74	dep	cell	62:65	arg1	fibrosis					76:83	in vitro fibrosis	67:83	in vitro fibrosis	67:83	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	7	75	theme	matrix	1236:1241	arg1	mineralization					1243:1256	sporadic matrix mineralization	1227:1256	sporadic matrix mineralization	1227:1256	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	4	76	theme	3D	723:724	arg1	cylinders					726:734	PCL 3D cylinders	719:734	PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium	719:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	9	77	theme	MSC-derived	1740:1750	arg1	fibroblasts					1752:1762	MSC-derived fibroblasts	1740:1762	MSC-derived fibroblasts	1740:1762	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	4	78	theme	2	780:780	arg1	%					781:781	%	781:781	%	781:781	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	7	79	theme	surface	1321:1327	arg1	layers					1329:1334	highly cellular surface layers	1305:1334	highly cellular surface layers that enveloped all scaffolds	1305:1363	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	3	80	theme	mesenchymal	455:465	arg1	MSCs					479:482	MSCs	479:482	MSCs	479:482	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	3	80	theme	mesenchymal	455:465	arg1	cells					472:476	human bone marrow mesenchymal stem cells	437:476	human bone marrow mesenchymal stem cells (MSCs)	437:483	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	3	81	with	scaffolds	527:535	arg1	pores					576:580	fully interconnected 84 or 141 µm pores	542:580	fully interconnected 84 or 141 µm pores	542:580	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	7	82	theme	osteogenic	1208:1217	arg1	medium					1219:1224	osteogenic medium	1208:1224	osteogenic medium	1208:1224	After 21 days of culture in osteogenic medium, sporadic matrix mineralization was detected histologically and by micro-CT in highly cellular surface layers that enveloped all scaffolds and in cell aggregates in 141 µm pores near the edges.
25504184	6	83	theme	Most	1060:1063	arg1	pores					1065:1069	Most pores	1060:1069	Most pores	1060:1069	Most pores, with or without chitosan, became filled by collagen networks sparsely populated with fibroblast-like cells.
25504184	9	84	theme	terminal	1778:1785	arg1	differentiation					1787:1801	osteoblast terminal differentiation	1767:1801	osteoblast terminal differentiation	1767:1801	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	4	85	theme	cold	757:760	arg1	method					770:775	a novel static cold seeding method	742:775	a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium	742:942	Freshly trypsinized MSCs were seeded on PCL 3D cylinders using a novel static cold seeding method in 2% serum to optimally populate all depths of the scaffold discs, followed by 10 days of culture in proliferation medium and 21 additional days in osteogenic medium.
25504184	2	86	theme	cell-surface	349:360	arg1	attachment					362:371	cell-surface attachment	349:371	cell-surface attachment	349:371	Limited information is available on the role of internal geometry and cell-surface attachment on osseous integration potential.
25504184	1	87	theme	hydrophobic	196:206	arg1	bioplastic					208:217	a hydrophobic bioplastic	194:217	a hydrophobic bioplastic under development for bone tissue engineering applications	194:276	Poly(epsilon-caprolactone) (PCL) is a hydrophobic bioplastic under development for bone tissue engineering applications.
25504184	1	87	theme	hydrophobic	196:206	arg1	Poly					158:161	Poly	158:161	Poly(epsilon-caprolactone) (PCL)	158:189	Poly(epsilon-caprolactone) (PCL) is a hydrophobic bioplastic under development for bone tissue engineering applications.
25504184	9	88	from	formation	1671:1679	arg1	pores					1690:1694	84 µm pores	1684:1694	84 µm pores	1684:1694	This study revealed that LbL-chitosan coatings are sufficient to promote MSC attachment to PCL but only enhance mineral formation in 84 µm pores, suggesting a potential inhibitory role for MSC-derived fibroblasts in osteoblast terminal differentiation.
25504184	0	89	dep	size	5:8	arg1	coating					27:33	coating	27:33	coating	27:33	Pore size and LbL chitosan coating influence mesenchymal stem cell in vitro fibrosis and biomineralization in 3D porous poly(epsilon-caprolactone) scaffolds.
25504184	3	90	theme	bone	443:446	arg1	marrow					448:453	human bone marrow	437:453	human bone marrow mesenchymal stem cells (MSCs)	437:483	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
25504184	3	91	theme	porous	513:518	arg1	scaffolds					527:535	porous 3D PCL scaffolds	513:535	porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes	513:676	We tested the hypothesis that human bone marrow mesenchymal stem cells (MSCs) deposit more mineral inside porous 3D PCL scaffolds with fully interconnected 84 or 141 µm pores, when the surfaces are coated with chitosan via Layer-by-Layer (LbL)-deposited polyelectrolytes.
28796321	3	0	theme	food	550:553	arg1	matrices					555:562	food matrices	550:562	food matrices	550:562	A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices.
28796321	6	1	dep	%	970:970	arg1	to					972:973	to	972:973	to	972:973	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	0	2	theme	Pectin	65:70	arg1	Structure					52:60	the Structure	48:60	the Structure of Pectin	48:70	Low-Temperature Blanching as a Tool to Modulate the Structure of Pectin in Blueberry Purees.
28796321	3	3	theme	other	526:530	arg1	components					532:541	other components	526:541	other components within food matrices	526:562	A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices.
28796321	5	4	theme	pectin	879:884	arg1	depolymerization					886:901	any significant pectin depolymerization	863:901	any significant pectin depolymerization	863:901	The results showed that neither the amount of fiber, nor the viscosity were affected by LTB, indicating that this treatment did not result in any significant pectin depolymerization and degradation.
28796321	6	5	theme	total	1077:1081	arg1	pectin					1083:1088	total pectin	1077:1088	total pectin	1077:1088	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	4	6	theme	pasteurized	670:680	arg1	purees					713:718	both pasteurized, and LTB+pasteurized blueberry purees	665:718	both pasteurized, and LTB+pasteurized blueberry purees	665:718	Fiber content, viscosity, pectin solubility, DM, and monosaccharide composition were determined for both pasteurized, and LTB+pasteurized blueberry purees.
28796321	7	7	theme	low-methylated	1192:1205	arg1	pectin					1207:1212	mostly soluble and low-methylated pectin	1173:1212	mostly soluble and low-methylated pectin	1173:1212	A LTB is a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients.
28796321	3	8	theme	puree	415:419	arg1	properties					421:430	puree properties	415:430	puree properties	415:430	A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices.
28796321	2	9	theme	%	219:219	arg1	%					225:225	4% to 7%	218:225	4% to 7%	218:225	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	9	theme	%	219:219	arg1	pectin					210:215	pectin	210:215	pectin (4% to 7%)	210:226	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	7	10	with	interactions	1269:1280	arg1	ingredients					1298:1308	other food ingredients	1287:1308	other food ingredients	1287:1308	A LTB is a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients.
28796321	3	11	theme	blueberry	354:362	arg1	purees					364:369	blueberry purees	354:369	blueberry purees	354:369	A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices.
28796321	7	12	theme	soluble	1180:1186	arg1	pectin					1207:1212	mostly soluble and low-methylated pectin	1173:1212	mostly soluble and low-methylated pectin	1173:1212	A LTB is a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients.
28796321	6	13	from	%	980:980	arg1	DM					957:958	pectin DM	950:958	pectin DM from 58-67% to 45-47%	950:980	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	2	14	contain	contains	153:160	arg2	fiber					187:191	dietary fiber	179:191	dietary fiber (10% to 20%)	179:204	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	14	contain	contains	153:160	arg2	%					203:203	10% to 20%	194:203	10% to 20%	194:203	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	14	contain	contains	153:160	arg2	pectin					210:215	pectin	210:215	pectin (4% to 7%)	210:226	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	14	contain	contains	153:160	arg2	%					225:225	4% to 7%	218:225	4% to 7%	218:225	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	14	contain	contains	153:160	arg1	It					150:151	It	150:151	It	150:151	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	14	contain	contains	153:160	arg2	amount					169:174	a good amount	162:174	a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing	162:291	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	6	15	theme	pectin	950:955	arg1	DM					957:958	pectin DM	950:958	pectin DM from 58-67% to 45-47%	950:980	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	2	16	dep	%	203:203	arg1	to					198:199	to	198:199	to	198:199	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	6	17	dep	%	1095:1095	arg1	to					1097:1098	to	1097:1098	to	1097:1098	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	4	18	theme	blueberry	703:711	arg1	purees					713:718	both pasteurized, and LTB+pasteurized blueberry purees	665:718	both pasteurized, and LTB+pasteurized blueberry purees	665:718	Fiber content, viscosity, pectin solubility, DM, and monosaccharide composition were determined for both pasteurized, and LTB+pasteurized blueberry purees.
28796321	6	19	theme	45-47	975:979	arg1	%					970:970	%	970:970	%	970:970	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	2	20	theme	food	277:280	arg1	processing					282:291	food processing	277:291	food processing	277:291	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	0	21	theme	Low-Temperature	0:14	arg1	Blanching					16:24	Low-Temperature Blanching	0:24	Low-Temperature Blanching as a Tool to Modulate the Structure of Pectin in Blueberry Purees.	0:91	Low-Temperature Blanching as a Tool to Modulate the Structure of Pectin in Blueberry Purees.
28796321	3	22	dep	blanching	312:320	arg1	LTB					323:325	LTB	323:325	LTB: 60 °C/1 h	323:336	A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices.
28796321	7	23	theme	simple	1116:1121	arg1	process					1132:1138	a simple and mild process	1114:1138	a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients	1114:1308	A LTB is a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients.
28796321	7	23	theme	simple	1116:1121	arg1	LTB					1107:1109	A LTB	1105:1109	A LTB	1105:1109	A LTB is a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients.
28796321	6	24	theme	pectin	1083:1088	arg1	fraction					1065:1072	the major fraction	1055:1072	the major fraction of total pectin	1055:1088	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	7	25	theme	mild	1127:1130	arg1	process					1132:1138	a simple and mild process	1114:1138	a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients	1114:1308	A LTB is a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients.
28796321	7	25	theme	mild	1127:1130	arg1	LTB					1107:1109	A LTB	1105:1109	A LTB	1105:1109	A LTB is a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients.
28796321	6	26	theme	fraction	1024:1031	arg1	amount					993:998	the amount	989:998	the amount of water-soluble pectin fraction	989:1031	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	6	26	theme	fraction	1024:1031	arg1	fraction					1024:1031	water-soluble pectin fraction	1003:1031	water-soluble pectin fraction	1003:1031	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	4	27	theme	pectin	591:596	arg1	solubility					598:607	pectin solubility	591:607	pectin solubility	591:607	Fiber content, viscosity, pectin solubility, DM, and monosaccharide composition were determined for both pasteurized, and LTB+pasteurized blueberry purees.
28796321	1	28	theme	Blueberry	93:101	arg1	composition					103:113	Blueberry composition	93:113	Blueberry composition	93:113	Blueberry composition was characterized for 6 cultivars.
28796321	3	29	theme	temperature	300:310	arg1	blanching					312:320	A low temperature blanching	294:320	A low temperature blanching (LTB: 60 °C/1 h)	294:337	A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices.
28796321	2	30	theme	good	164:167	arg1	%					225:225	4% to 7%	218:225	4% to 7%	218:225	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	30	theme	good	164:167	arg1	amount					169:174	a good amount	162:174	a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing	162:291	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	30	theme	good	164:167	arg1	fiber					187:191	dietary fiber	179:191	dietary fiber (10% to 20%)	179:204	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	30	theme	good	164:167	arg1	%					203:203	10% to 20%	194:203	10% to 20%	194:203	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	30	theme	good	164:167	arg1	pectin					210:215	pectin	210:215	pectin (4% to 7%)	210:226	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	7	31	theme	food	1293:1296	arg1	ingredients					1298:1308	other food ingredients	1287:1308	other food ingredients	1287:1308	A LTB is a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients.
28796321	3	32	theme	°C/1	331:334	arg1	h					336:336	60 °C/1 h	328:336	LTB: 60 °C/1 h	323:336	A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices.
28796321	6	33	theme	major	1059:1063	arg1	fraction					1065:1072	the major fraction	1055:1072	the major fraction of total pectin	1055:1088	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	3	34	dep	LTB	323:325	arg1	h					336:336	60 °C/1 h	328:336	LTB: 60 °C/1 h	323:336	A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices.
28796321	6	35	theme	57	1100:1101	arg1	%					1095:1095	%	1095:1095	%	1095:1095	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	5	36	theme	significant	867:877	arg1	depolymerization					886:901	any significant pectin depolymerization	863:901	any significant pectin depolymerization	863:901	The results showed that neither the amount of fiber, nor the viscosity were affected by LTB, indicating that this treatment did not result in any significant pectin depolymerization and degradation.
28796321	2	37	theme	%	196:196	arg1	fiber					187:191	dietary fiber	179:191	dietary fiber (10% to 20%)	179:204	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	37	theme	%	196:196	arg1	%					203:203	10% to 20%	194:203	10% to 20%	194:203	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	4	38	theme	LTB+pasteurized	687:701	arg1	purees					713:718	both pasteurized, and LTB+pasteurized blueberry purees	665:718	both pasteurized, and LTB+pasteurized blueberry purees	665:718	Fiber content, viscosity, pectin solubility, DM, and monosaccharide composition were determined for both pasteurized, and LTB+pasteurized blueberry purees.
28796321	2	39	dep	%	225:225	arg1	to					221:222	to	221:222	to	221:222	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	3	40	theme	low	296:298	arg1	blanching					312:320	A low temperature blanching	294:320	A low temperature blanching (LTB: 60 °C/1 h)	294:337	A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices.
28796321	7	41	theme	other	1287:1291	arg1	ingredients					1298:1308	other food ingredients	1287:1308	other food ingredients	1287:1308	A LTB is a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients.
28796321	0	42	dep	Tool	31:34	arg1	Modulate					39:46	Modulate	39:46	to Modulate the Structure of Pectin in Blueberry Purees	36:90	Low-Temperature Blanching as a Tool to Modulate the Structure of Pectin in Blueberry Purees.
28796321	2	43	theme	methylation	244:254	arg1	degree					234:239	degree	234:239	degree	234:239	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	44	theme	fiber	187:191	arg1	%					225:225	4% to 7%	218:225	4% to 7%	218:225	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	44	theme	fiber	187:191	arg1	amount					169:174	a good amount	162:174	a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing	162:291	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	44	theme	fiber	187:191	arg1	fiber					187:191	dietary fiber	179:191	dietary fiber (10% to 20%)	179:204	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	44	theme	fiber	187:191	arg1	%					203:203	10% to 20%	194:203	10% to 20%	194:203	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	44	theme	fiber	187:191	arg1	pectin					210:215	pectin	210:215	pectin (4% to 7%)	210:226	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	45	theme	pectin	210:215	arg1	%					225:225	4% to 7%	218:225	4% to 7%	218:225	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	45	theme	pectin	210:215	arg1	amount					169:174	a good amount	162:174	a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing	162:291	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	45	theme	pectin	210:215	arg1	fiber					187:191	dietary fiber	179:191	dietary fiber (10% to 20%)	179:204	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	45	theme	pectin	210:215	arg1	%					203:203	10% to 20%	194:203	10% to 20%	194:203	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	45	theme	pectin	210:215	arg1	pectin					210:215	pectin	210:215	pectin (4% to 7%)	210:226	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	5	46	theme	fiber	767:771	arg1	fiber					767:771	fiber	767:771	fiber	767:771	The results showed that neither the amount of fiber, nor the viscosity were affected by LTB, indicating that this treatment did not result in any significant pectin depolymerization and degradation.
28796321	5	46	theme	fiber	767:771	arg1	amount					757:762	the amount	753:762	the amount	753:762	The results showed that neither the amount of fiber, nor the viscosity were affected by LTB, indicating that this treatment did not result in any significant pectin depolymerization and degradation.
28796321	7	47	theme	blueberry	1151:1159	arg1	purees					1161:1166	blueberry purees	1151:1166	blueberry purees	1151:1166	A LTB is a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients.
28796321	6	48	theme	52	1093:1094	arg1	%					1095:1095	%	1095:1095	%	1095:1095	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	2	49	theme	dietary	179:185	arg1	fiber					187:191	dietary fiber	179:191	dietary fiber (10% to 20%)	179:204	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	2	49	theme	dietary	179:185	arg1	%					203:203	10% to 20%	194:203	10% to 20%	194:203	It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing.
28796321	6	50	theme	water-soluble	1003:1015	arg1	fraction					1024:1031	water-soluble pectin fraction	1003:1031	water-soluble pectin fraction	1003:1031	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	4	51	theme	Fiber	565:569	arg1	content					571:577	Fiber content	565:577	Fiber content	565:577	Fiber content, viscosity, pectin solubility, DM, and monosaccharide composition were determined for both pasteurized, and LTB+pasteurized blueberry purees.
28796321	4	52	theme	monosaccharide	618:631	arg1	composition					633:643	monosaccharide composition	618:643	monosaccharide composition	618:643	Fiber content, viscosity, pectin solubility, DM, and monosaccharide composition were determined for both pasteurized, and LTB+pasteurized blueberry purees.
28796321	3	53	theme	pectin	503:508	arg1	affinity					510:517	pectin affinity	503:517	pectin affinity toward other components within food matrices	503:562	A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices.
28796321	3	54	theme	pectin	383:388	arg1	DM					390:391	pectin DM	383:391	pectin DM	383:391	A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices.
28796321	6	55	theme	58-67	965:969	arg1	%					970:970	%	970:970	%	970:970	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
28796321	6	56	theme	pectin	1017:1022	arg1	fraction					1024:1031	water-soluble pectin fraction	1003:1031	water-soluble pectin fraction	1003:1031	LTB caused a decrease both in pectin DM from 58-67% to 45-47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%.
27519294	7	0	from	changes	788:794	arg1	groups					850:855	diabetic and hypercholesterolemic groups	816:855	diabetic and hypercholesterolemic groups	816:855	There were quantitative changes in sulfated GAGs in diabetic and hypercholesterolemic groups when compared to normal rats.
27519294	7	0	from	changes	788:794	arg1	GAGs					808:811	sulfated GAGs	799:811	sulfated GAGs	799:811	There were quantitative changes in sulfated GAGs in diabetic and hypercholesterolemic groups when compared to normal rats.
27519294	5	1	theme	Peritoneal	581:590	arg1	macrophages					592:602	Peritoneal macrophages	581:602	Peritoneal macrophages	581:602	Peritoneal macrophages are known to play important roles in the control of infection and inflammation.
27519294	10	2	theme	Global	1286:1291	arg1	analysis					1318:1325	Global disaccharide composition analysis	1286:1325	Global disaccharide composition analysis	1286:1325	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	1	3	contain	have	130:133	arg1	Glycosaminoglycans					104:121	Glycosaminoglycans	104:121	Glycosaminoglycans (GAGs)	104:128	Glycosaminoglycans (GAGs) have a plethora of functions to play.
27519294	1	3	contain	have	130:133	arg2	plethora					137:144	a plethora	135:144	a plethora of functions	135:157	Glycosaminoglycans (GAGs) have a plethora of functions to play.
27519294	1	3	contain	have	130:133	arg1	GAGs					124:127	GAGs	124:127	GAGs	124:127	Glycosaminoglycans (GAGs) have a plethora of functions to play.
27519294	4	4	theme	diabetic	429:436	arg1	rats					477:480	normal, diabetic, and diet-induced hypercholesterolemic rats	421:480	normal, diabetic, and diet-induced hypercholesterolemic rats	421:480	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	9	5	with	treatment	1159:1167	arg1	heparinase					1174:1183	heparinase	1174:1183	heparinase	1174:1183	Cytoadherence was significantly decreased on treatment with heparinase indicating that cytoadherence was at least partly mediated by heparan sulfate/heparin class of GAGs.
27519294	8	6	theme	type	1050:1053	arg1	collagen					1058:1065	type IV collagen	1050:1065	type IV collagen	1050:1065	Dose-dependent changes in cytoadherence were observed only with respect to fibronectin in LPS-activated macrophages from diabetic animals but not with laminin and type IV collagen when compared to macrophages from normal rats.
27519294	0	7	from	Effect	0:5	arg1	glycosaminoglycans					59:76	peritoneal macrophage glycosaminoglycans	37:76	peritoneal macrophage glycosaminoglycans	37:76	Effect of pathological conditions on peritoneal macrophage glycosaminoglycans: Impact on cytoadherence.
27519294	5	8	theme	infection	656:664	arg1	control					645:651	the control	641:651	the control of infection and inflammation	641:681	Peritoneal macrophages are known to play important roles in the control of infection and inflammation.
27519294	4	9	theme	peritoneal	379:388	arg1	macrophages					390:400	peritoneal macrophages	379:400	peritoneal macrophages	379:400	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	8	10	theme	diabetic	1008:1015	arg1	animals					1017:1023	diabetic animals	1008:1023	diabetic animals	1008:1023	Dose-dependent changes in cytoadherence were observed only with respect to fibronectin in LPS-activated macrophages from diabetic animals but not with laminin and type IV collagen when compared to macrophages from normal rats.
27519294	2	11	from	present	184:190	arg1	surface					222:228	extracellular matrix, cell surface	195:228	surface	222:228	They are widely present in extracellular matrix, cell surface and inside the cell.
27519294	8	12	located	observed	932:939	arg2	changes					902:908	Dose-dependent changes	887:908	Dose-dependent changes in cytoadherence	887:925	Dose-dependent changes in cytoadherence were observed only with respect to fibronectin in LPS-activated macrophages from diabetic animals but not with laminin and type IV collagen when compared to macrophages from normal rats.
27519294	8	12	located	observed	932:939	arg1	macrophages					991:1001	LPS-activated macrophages	977:1001	LPS-activated macrophages from diabetic animals	977:1023	Dose-dependent changes in cytoadherence were observed only with respect to fibronectin in LPS-activated macrophages from diabetic animals but not with laminin and type IV collagen when compared to macrophages from normal rats.
27519294	9	13	theme	sulfate/heparin	1255:1269	arg1	class					1271:1275	heparan sulfate/heparin class	1247:1275	heparan sulfate/heparin class of GAGs	1247:1283	Cytoadherence was significantly decreased on treatment with heparinase indicating that cytoadherence was at least partly mediated by heparan sulfate/heparin class of GAGs.
27519294	10	14	theme	sulfation	1392:1400	arg1	ratio					1402:1406	higher sulfation ratio	1385:1406	higher sulfation ratio	1385:1406	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	5	15	theme	important	622:630	arg1	roles					632:636	important roles	622:636	important roles	622:636	Peritoneal macrophages are known to play important roles in the control of infection and inflammation.
27519294	2	16	located	present	184:190	arg1	surface					222:228	extracellular matrix, cell surface	195:228	surface	222:228	They are widely present in extracellular matrix, cell surface and inside the cell.
27519294	2	16	located	present	184:190	arg2	They					168:171	They	168:171	They	168:171	They are widely present in extracellular matrix, cell surface and inside the cell.
27519294	4	17	theme	present	365:371	arg1	work					373:376	the present work	361:376	the present work	361:376	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	7	18	theme	normal	874:879	arg1	rats					881:884	normal rats	874:884	normal rats	874:884	There were quantitative changes in sulfated GAGs in diabetic and hypercholesterolemic groups when compared to normal rats.
27519294	7	19	theme	diabetic	816:823	arg1	groups					850:855	diabetic and hypercholesterolemic groups	816:855	diabetic and hypercholesterolemic groups	816:855	There were quantitative changes in sulfated GAGs in diabetic and hypercholesterolemic groups when compared to normal rats.
27519294	9	20	theme	GAGs	1280:1283	arg1	class					1271:1275	heparan sulfate/heparin class	1247:1275	heparan sulfate/heparin class of GAGs	1247:1283	Cytoadherence was significantly decreased on treatment with heparinase indicating that cytoadherence was at least partly mediated by heparan sulfate/heparin class of GAGs.
27519294	2	21	from	surface	222:228	arg1	present					184:190	present	184:190	present	184:190	They are widely present in extracellular matrix, cell surface and inside the cell.
27519294	2	22	attach	present	184:190	arg1	surface					222:228	extracellular matrix, cell surface	195:228	surface	222:228	They are widely present in extracellular matrix, cell surface and inside the cell.
27519294	2	22	attach	present	184:190	arg2	They					168:171	They	168:171	They	168:171	They are widely present in extracellular matrix, cell surface and inside the cell.
27519294	4	23	theme	normal	421:426	arg1	rats					477:480	normal, diabetic, and diet-induced hypercholesterolemic rats	421:480	normal, diabetic, and diet-induced hypercholesterolemic rats	421:480	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	2	24	theme	matrix	209:214	arg1	surface					222:228	extracellular matrix, cell surface	195:228	surface	222:228	They are widely present in extracellular matrix, cell surface and inside the cell.
27519294	10	25	theme	control	1433:1439	arg1	animals					1466:1472	control and hypercholesterolemic animals	1433:1472	control and hypercholesterolemic animals	1433:1472	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	0	26	theme	conditions	23:32	arg1	Effect					0:5	Effect	0:5	Effect of pathological conditions on peritoneal macrophage glycosaminoglycans: Impact on cytoadherence.	0:102	Effect of pathological conditions on peritoneal macrophage glycosaminoglycans: Impact on cytoadherence.
27519294	4	27	theme	matrix	556:561	arg1	components					569:578	various extracellular matrix (ECM) components	534:578	various extracellular matrix (ECM) components	534:578	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	8	28	theme	normal	1101:1106	arg1	rats					1108:1111	normal rats	1101:1111	normal rats	1101:1111	Dose-dependent changes in cytoadherence were observed only with respect to fibronectin in LPS-activated macrophages from diabetic animals but not with laminin and type IV collagen when compared to macrophages from normal rats.
27519294	6	29	theme	Isolated	684:691	arg1	GAGs					693:696	Isolated GAGs	684:696	Isolated GAGs	684:696	Isolated GAGs were characterized as belonging to heparan sulfate/heparin class.
27519294	8	30	from	animals	1017:1023	arg1	macrophages					991:1001	LPS-activated macrophages	977:1001	LPS-activated macrophages from diabetic animals	977:1023	Dose-dependent changes in cytoadherence were observed only with respect to fibronectin in LPS-activated macrophages from diabetic animals but not with laminin and type IV collagen when compared to macrophages from normal rats.
27519294	4	31	attach	isolated	407:414	arg2	macrophages					390:400	peritoneal macrophages	379:400	peritoneal macrophages	379:400	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	4	31	attach	isolated	407:414	arg1	rats					477:480	normal, diabetic, and diet-induced hypercholesterolemic rats	421:480	normal, diabetic, and diet-induced hypercholesterolemic rats	421:480	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	10	32	theme	animals	1373:1379	arg1	macrophages					1349:1359	macrophages	1349:1359	macrophages of diabetic animals	1349:1379	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	10	33	contain	had	1381:1383	arg1	GAGs					1339:1342	GAGs	1339:1342	GAGs from macrophages of diabetic animals	1339:1379	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	10	33	contain	had	1381:1383	arg2	ratio					1402:1406	higher sulfation ratio	1385:1406	higher sulfation ratio	1385:1406	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	0	34	theme	peritoneal	37:46	arg1	glycosaminoglycans					59:76	peritoneal macrophage glycosaminoglycans	37:76	peritoneal macrophage glycosaminoglycans	37:76	Effect of pathological conditions on peritoneal macrophage glycosaminoglycans: Impact on cytoadherence.
27519294	4	35	theme	ECM	564:566	arg1	components					569:578	various extracellular matrix (ECM) components	534:578	various extracellular matrix (ECM) components	534:578	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	8	36	theme	Dose-dependent	887:900	arg1	changes					902:908	Dose-dependent changes	887:908	Dose-dependent changes in cytoadherence	887:925	Dose-dependent changes in cytoadherence were observed only with respect to fibronectin in LPS-activated macrophages from diabetic animals but not with laminin and type IV collagen when compared to macrophages from normal rats.
27519294	10	37	theme	higher	1385:1390	arg1	ratio					1402:1406	higher sulfation ratio	1385:1406	higher sulfation ratio	1385:1406	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	0	38	theme	pathological	10:21	arg1	conditions					23:32	pathological conditions	10:32	pathological conditions	10:32	Effect of pathological conditions on peritoneal macrophage glycosaminoglycans: Impact on cytoadherence.
27519294	7	39	theme	sulfated	799:806	arg1	GAGs					808:811	sulfated GAGs	799:811	sulfated GAGs	799:811	There were quantitative changes in sulfated GAGs in diabetic and hypercholesterolemic groups when compared to normal rats.
27519294	3	40	theme	pathological	258:269	arg1	conditions					271:280	pathological conditions	258:280	pathological conditions	258:280	During pathological conditions remodeling of GAGs leads to modifications in their structure and functions.
27519294	0	41	from	Impact	79:84	arg1	cytoadherence					89:101	cytoadherence	89:101	cytoadherence	89:101	Effect of pathological conditions on peritoneal macrophage glycosaminoglycans: Impact on cytoadherence.
27519294	2	42	dep	surface	222:228	arg1	cell					217:220	extracellular matrix, cell surface	195:228	cell	217:220	They are widely present in extracellular matrix, cell surface and inside the cell.
27519294	10	43	theme	diabetic	1364:1371	arg1	animals					1373:1379	diabetic animals	1364:1379	diabetic animals	1364:1379	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	8	44	from	rats	1108:1111	arg1	macrophages					1084:1094	macrophages	1084:1094	macrophages from normal rats	1084:1111	Dose-dependent changes in cytoadherence were observed only with respect to fibronectin in LPS-activated macrophages from diabetic animals but not with laminin and type IV collagen when compared to macrophages from normal rats.
27519294	5	45	theme	inflammation	670:681	arg1	control					645:651	the control	641:651	the control of infection and inflammation	641:681	Peritoneal macrophages are known to play important roles in the control of infection and inflammation.
27519294	7	46	theme	hypercholesterolemic	829:848	arg1	groups					850:855	diabetic and hypercholesterolemic groups	816:855	diabetic and hypercholesterolemic groups	816:855	There were quantitative changes in sulfated GAGs in diabetic and hypercholesterolemic groups when compared to normal rats.
27519294	0	47	theme	macrophage	48:57	arg1	glycosaminoglycans					59:76	peritoneal macrophage glycosaminoglycans	37:76	peritoneal macrophage glycosaminoglycans	37:76	Effect of pathological conditions on peritoneal macrophage glycosaminoglycans: Impact on cytoadherence.
27519294	4	48	theme	extracellular	542:554	arg1	components					569:578	various extracellular matrix (ECM) components	534:578	various extracellular matrix (ECM) components	534:578	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	3	49	from	modifications	310:322	arg1	functions					347:355	functions	347:355	functions	347:355	During pathological conditions remodeling of GAGs leads to modifications in their structure and functions.
27519294	3	49	from	modifications	310:322	arg1	structure					333:341	structure	333:341	structure	333:341	During pathological conditions remodeling of GAGs leads to modifications in their structure and functions.
27519294	8	50	from	changes	902:908	arg1	cytoadherence					913:925	cytoadherence	913:925	cytoadherence	913:925	Dose-dependent changes in cytoadherence were observed only with respect to fibronectin in LPS-activated macrophages from diabetic animals but not with laminin and type IV collagen when compared to macrophages from normal rats.
27519294	8	51	theme	LPS-activated	977:989	arg1	macrophages					991:1001	LPS-activated macrophages	977:1001	LPS-activated macrophages from diabetic animals	977:1023	Dose-dependent changes in cytoadherence were observed only with respect to fibronectin in LPS-activated macrophages from diabetic animals but not with laminin and type IV collagen when compared to macrophages from normal rats.
27519294	4	52	theme	hypercholesterolemic	456:475	arg1	rats					477:480	normal, diabetic, and diet-induced hypercholesterolemic rats	421:480	normal, diabetic, and diet-induced hypercholesterolemic rats	421:480	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	4	53	theme	various	534:540	arg1	components					569:578	various extracellular matrix (ECM) components	534:578	various extracellular matrix (ECM) components	534:578	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	2	54	theme	extracellular	195:207	arg1	surface					222:228	extracellular matrix, cell surface	195:228	surface	222:228	They are widely present in extracellular matrix, cell surface and inside the cell.
27519294	9	55	theme	heparan	1247:1253	arg1	class					1271:1275	heparan sulfate/heparin class	1247:1275	heparan sulfate/heparin class of GAGs	1247:1283	Cytoadherence was significantly decreased on treatment with heparinase indicating that cytoadherence was at least partly mediated by heparan sulfate/heparin class of GAGs.
27519294	10	56	from	macrophages	1349:1359	arg1	GAGs					1339:1342	GAGs	1339:1342	GAGs from macrophages of diabetic animals	1339:1379	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	3	57	theme	GAGs	296:299	arg1	remodeling					282:291	remodeling	282:291	remodeling of GAGs	282:299	During pathological conditions remodeling of GAGs leads to modifications in their structure and functions.
27519294	4	58	theme	GAGs	508:511	arg1	cytoadherence					517:529	cytoadherence	517:529	cytoadherence to various extracellular matrix (ECM) components	517:578	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	4	58	theme	GAGs	508:511	arg1	terms					499:503	terms	499:503	terms of GAGs	499:511	In the present work, peritoneal macrophages were isolated from normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs and cytoadherence to various extracellular matrix (ECM) components.
27519294	10	59	theme	composition	1306:1316	arg1	analysis					1318:1325	Global disaccharide composition analysis	1286:1325	Global disaccharide composition analysis	1286:1325	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	7	60	theme	quantitative	775:786	arg1	changes					788:794	quantitative changes	775:794	quantitative changes in sulfated GAGs in diabetic and hypercholesterolemic groups	775:855	There were quantitative changes in sulfated GAGs in diabetic and hypercholesterolemic groups when compared to normal rats.
27519294	10	61	theme	hypercholesterolemic	1445:1464	arg1	animals					1466:1472	control and hypercholesterolemic animals	1433:1472	control and hypercholesterolemic animals	1433:1472	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	6	62	theme	sulfate/heparin	741:755	arg1	class					757:761	heparan sulfate/heparin class	733:761	heparan sulfate/heparin class	733:761	Isolated GAGs were characterized as belonging to heparan sulfate/heparin class.
27519294	10	63	theme	disaccharide	1293:1304	arg1	analysis					1318:1325	Global disaccharide composition analysis	1286:1325	Global disaccharide composition analysis	1286:1325	Global disaccharide composition analysis showed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared to that of control and hypercholesterolemic animals.
27519294	0	64	dep	Effect	0:5	arg1	Impact					79:84	Impact	79:84	Effect of pathological conditions on peritoneal macrophage glycosaminoglycans: Impact on cytoadherence.	0:102	Effect of pathological conditions on peritoneal macrophage glycosaminoglycans: Impact on cytoadherence.
27519294	6	65	theme	heparan	733:739	arg1	class					757:761	heparan sulfate/heparin class	733:761	heparan sulfate/heparin class	733:761	Isolated GAGs were characterized as belonging to heparan sulfate/heparin class.
27519294	1	66	theme	functions	149:157	arg1	plethora					137:144	a plethora	135:144	a plethora of functions	135:157	Glycosaminoglycans (GAGs) have a plethora of functions to play.
29143524	13	0	theme	potential	2185:2193	arg1	candidate					2195:2203	a potential candidate	2183:2203	a potential candidate for craniofacial bone defect regeneration	2183:2245	This shows that the CaSO4 and FGF-18-incorporated hydrogel is a potential candidate for craniofacial bone defect regeneration.
29143524	13	0	theme	potential	2185:2193	arg1	CaSO4					2141:2145	CaSO4	2141:2145	CaSO4	2141:2145	This shows that the CaSO4 and FGF-18-incorporated hydrogel is a potential candidate for craniofacial bone defect regeneration.
29143524	13	0	theme	potential	2185:2193	arg1	hydrogel					2171:2178	FGF-18-incorporated hydrogel	2151:2178	FGF-18-incorporated hydrogel	2151:2178	This shows that the CaSO4 and FGF-18-incorporated hydrogel is a potential candidate for craniofacial bone defect regeneration.
29143524	1	1	theme	handling	308:315	arg1	scaffolds					237:245	conventional scaffolds	224:245	conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues	224:372	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	4	2	theme	regeneration	899:910	arg1	chemistry					912:920	regeneration chemistry	899:920	regeneration chemistry	899:920	We developed a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel by regeneration chemistry and then incorporated CaSO4 and FGF-18 for this purpose.
29143524	13	3	theme	bone	2222:2225	arg1	regeneration					2234:2245	craniofacial bone defect regeneration	2209:2245	craniofacial bone defect regeneration	2209:2245	This shows that the CaSO4 and FGF-18-incorporated hydrogel is a potential candidate for craniofacial bone defect regeneration.
29143524	8	4	theme	enhanced	1319:1326	arg1	expression					1355:1364	an enhanced alkaline phosphatase (ALP) expression	1316:1364	an enhanced alkaline phosphatase (ALP) expression in the FGF-18-containing chitin-PLGA/CaSO4 gel	1316:1411	In vitro osteogenic differentiation showed an enhanced alkaline phosphatase (ALP) expression in the FGF-18-containing chitin-PLGA/CaSO4 gel when compared to cells alone.
29143524	0	5	theme	Injectable	0:9	arg1	Hydrogel					64:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel	0:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel	0:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model.
29143524	11	6	theme	longitudinal	1828:1839	arg1	μ-CT					1853:1856	longitudinal live animal μ-CT	1828:1856	longitudinal live animal μ-CT	1828:1856	Further, the regeneration potential of the prepared hydrogels was tested in vivo, and longitudinal live animal μ-CT was performed.
29143524	8	7	theme	phosphatase	1337:1347	arg1	expression					1355:1364	an enhanced alkaline phosphatase (ALP) expression	1316:1364	an enhanced alkaline phosphatase (ALP) expression in the FGF-18-containing chitin-PLGA/CaSO4 gel	1316:1411	In vitro osteogenic differentiation showed an enhanced alkaline phosphatase (ALP) expression in the FGF-18-containing chitin-PLGA/CaSO4 gel when compared to cells alone.
29143524	12	8	theme	control	2031:2037	arg1	systems					2039:2045	sham control systems	2026:2045	sham control systems	2026:2045	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	8	9	theme	vitro osteogenic	1276:1291	arg1	differentiation					1293:1307	vitro osteogenic differentiation	1276:1307	vitro osteogenic differentiation	1276:1307	In vitro osteogenic differentiation showed an enhanced alkaline phosphatase (ALP) expression in the FGF-18-containing chitin-PLGA/CaSO4 gel when compared to cells alone.
29143524	2	10	theme	poor	561:564	arg1	cost-efficiency					566:580	poor cost-efficiency	561:580	poor cost-efficiency	561:580	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	11	11	theme	animal	1846:1851	arg1	μ-CT					1853:1856	longitudinal live animal μ-CT	1828:1856	longitudinal live animal μ-CT	1828:1856	Further, the regeneration potential of the prepared hydrogels was tested in vivo, and longitudinal live animal μ-CT was performed.
29143524	9	12	theme	osteogenic	1499:1508	arg1	ALP					1524:1526	ALP	1524:1526	ALP	1524:1526	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	12	theme	osteogenic	1499:1508	arg1	BMP-2					1529:1533	BMP-2	1529:1533	BMP-2	1529:1533	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	12	theme	osteogenic	1499:1508	arg1	osteopontin					1559:1569	osteopontin	1559:1569	osteopontin (OPN)	1559:1575	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	12	theme	osteogenic	1499:1508	arg1	genes					1510:1514	osteogenic genes	1499:1514	osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)]	1499:1576	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	12	theme	osteogenic	1499:1508	arg1	[RUNX2					1516:1521	[RUNX2	1516:1521	[RUNX2	1516:1521	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	12	theme	osteogenic	1499:1508	arg1	osteocalcin					1536:1546	osteocalcin	1536:1546	osteocalcin (OCN)	1536:1552	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	7	13	theme	Chitin-PLGA/CaSO4	1215:1231	arg1	gel					1233:1235	Chitin-PLGA/CaSO4 gel	1215:1235	Chitin-PLGA/CaSO4 gel	1215:1235	Chitin-PLGA/CaSO4 gel showed sustained release of FGF-18.
29143524	1	14	theme	improved	264:271	arg1	adaptability					287:298	their improved defect margin adaptability	258:298	their improved defect margin adaptability	258:298	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	13	15	theme	defect	2227:2232	arg1	regeneration					2234:2245	craniofacial bone defect regeneration	2209:2245	craniofacial bone defect regeneration	2209:2245	This shows that the CaSO4 and FGF-18-incorporated hydrogel is a potential candidate for craniofacial bone defect regeneration.
29143524	3	16	theme	BMP-2	782:786	arg1	expression					788:797	the BMP-2 expression	778:797	the BMP-2 expression by supressing noggin	778:818	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
29143524	5	17	theme	CaSO4-incorporated	1055:1072	arg1	hydrogels					1086:1094	the CaSO4-incorporated chitin-PLGA hydrogels	1051:1094	the CaSO4-incorporated chitin-PLGA hydrogels	1051:1094	Rheologically, a 7-fold increase in the elastic modulus was observed in the CaSO4-incorporated chitin-PLGA hydrogels as compared to the chitin-PLGA hydrogel.
29143524	1	18	theme	bone	152:155	arg1	regeneration					157:168	craniofacial bone regeneration	139:168	craniofacial bone regeneration	139:168	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	2	19	from	variations	538:547	arg1	doses					604:608	supraphysiological doses	585:608	supraphysiological doses	585:608	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	8	20	theme	chitin-PLGA/CaSO4	1391:1407	arg1	gel					1409:1411	the FGF-18-containing chitin-PLGA/CaSO4 gel	1369:1411	the FGF-18-containing chitin-PLGA/CaSO4 gel	1369:1411	In vitro osteogenic differentiation showed an enhanced alkaline phosphatase (ALP) expression in the FGF-18-containing chitin-PLGA/CaSO4 gel when compared to cells alone.
29143524	8	21	from	expression	1355:1364	arg1	gel					1409:1411	the FGF-18-containing chitin-PLGA/CaSO4 gel	1369:1411	the FGF-18-containing chitin-PLGA/CaSO4 gel	1369:1411	In vitro osteogenic differentiation showed an enhanced alkaline phosphatase (ALP) expression in the FGF-18-containing chitin-PLGA/CaSO4 gel when compared to cells alone.
29143524	2	22	theme	interindividual	522:536	arg1	variations					538:547	interindividual variations	522:547	interindividual variations	522:547	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	2	23	from	edema	550:554	arg1	doses					604:608	supraphysiological doses	585:608	supraphysiological doses	585:608	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	12	24	theme	osteoid	2090:2096	arg1	stainings					2110:2118	osteoid tetrachrome stainings	2090:2118	osteoid tetrachrome stainings	2090:2118	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	11	25	theme	regeneration	1755:1766	arg1	potential					1768:1776	the regeneration potential	1751:1776	the regeneration potential of the prepared hydrogels	1751:1802	Further, the regeneration potential of the prepared hydrogels was tested in vivo, and longitudinal live animal μ-CT was performed.
29143524	13	26	theme	craniofacial	2209:2220	arg1	regeneration					2234:2245	craniofacial bone defect regeneration	2209:2245	craniofacial bone defect regeneration	2209:2245	This shows that the CaSO4 and FGF-18-incorporated hydrogel is a potential candidate for craniofacial bone defect regeneration.
29143524	1	27	theme	injectable	186:195	arg1	hydrogels					197:205	shear-thinning injectable hydrogels	171:205	shear-thinning injectable hydrogels	171:205	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	0	28	theme	Cranial	108:114	arg1	Model					128:132	Mice Cranial Bone Defect Model	103:132	Mice Cranial Bone Defect Model	103:132	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model.
29143524	11	29	theme	prepared	1785:1792	arg1	hydrogels					1794:1802	the prepared hydrogels	1781:1802	the prepared hydrogels	1781:1802	Further, the regeneration potential of the prepared hydrogels was tested in vivo, and longitudinal live animal μ-CT was performed.
29143524	0	30	theme	Defect	121:126	arg1	Model					128:132	Mice Cranial Bone Defect Model	103:132	Mice Cranial Bone Defect Model	103:132	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model.
29143524	9	31	theme	immunofluorescence	1579:1596	arg1	staining					1598:1605	immunofluorescence staining	1579:1605	immunofluorescence staining of BMP-2, OCN, and OPN	1579:1628	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	10	32	theme	FGF-18	1677:1682	arg1	Incorporation					1660:1672	Incorporation	1660:1672	Incorporation of FGF-18 in the hydrogel	1660:1698	Incorporation of FGF-18 in the hydrogel increased the endothelial cell migration.
29143524	7	33	theme	sustained	1244:1252	arg1	release					1254:1260	sustained release	1244:1260	sustained release of FGF-18	1244:1270	Chitin-PLGA/CaSO4 gel showed sustained release of FGF-18.
29143524	1	34	theme	ability	322:328	arg1	scaffolds					237:245	conventional scaffolds	224:245	conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues	224:372	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	12	35	theme	bone	1938:1941	arg1	healing					1943:1949	early and almost complete bone healing	1912:1949	early and almost complete bone healing	1912:1949	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	2	36	theme	protein-2	472:480	arg1	use					428:430	the use	424:430	the use of recombinant human bone morphogenetic protein-2 (BMP-2)	424:488	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	2	36	theme	protein-2	472:480	arg1	method					393:398	The most accepted method	375:398	The most accepted method	375:398	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	2	37	theme	bone	453:456	arg1	BMP-2					483:487	BMP-2	483:487	BMP-2	483:487	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	2	37	theme	bone	453:456	arg1	protein-2					472:480	recombinant human bone morphogenetic protein-2	435:480	recombinant human bone morphogenetic protein-2 (BMP-2)	435:488	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	0	38	theme	CaSO4/FGF-18-Incorporated	26:50	arg1	Hydrogel					64:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel	0:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel	0:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model.
29143524	12	39	with	comparison	1954:1963	arg1	systems					2039:2045	sham control systems	2026:2045	sham control systems	2026:2045	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	12	39	with	comparison	1954:1963	arg1	chitin-PLGA					2009:2019	chitin-PLGA	2009:2019	chitin-PLGA	2009:2019	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	12	39	with	comparison	1954:1963	arg1	chitin-PLGA/FGF-18					1989:2006	chitin-PLGA/FGF-18	1989:2006	chitin-PLGA/FGF-18	1989:2006	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	12	39	with	comparison	1954:1963	arg1	chitin-PLGA/CaSO4					1970:1986	chitin-PLGA/CaSO4	1970:1986	chitin-PLGA/CaSO4	1970:1986	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	4	40	theme	chitin-poly	836:846	arg1	hydrogel					887:894	a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel	834:894	a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel by regeneration chemistry	834:920	We developed a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel by regeneration chemistry and then incorporated CaSO4 and FGF-18 for this purpose.
29143524	2	41	theme	recombinant	435:445	arg1	BMP-2					483:487	BMP-2	483:487	BMP-2	483:487	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	2	41	theme	recombinant	435:445	arg1	protein-2					472:480	recombinant human bone morphogenetic protein-2	435:480	recombinant human bone morphogenetic protein-2 (BMP-2)	435:488	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	12	42	theme	early	1912:1916	arg1	healing					1943:1949	early and almost complete bone healing	1912:1949	early and almost complete bone healing	1912:1949	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	9	43	theme	OPN	1626:1628	arg1	staining					1598:1605	immunofluorescence staining	1579:1605	immunofluorescence staining of BMP-2, OCN, and OPN	1579:1628	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	43	theme	OPN	1626:1628	arg1	expression					1485:1494	the expression	1481:1494	the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)]	1481:1576	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	43	theme	OPN	1626:1628	arg1	staining					1650:1657	alizarin red S staining	1635:1657	alizarin red S staining	1635:1657	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	10	44	from	Incorporation	1660:1672	arg1	hydrogel					1691:1698	the hydrogel	1687:1698	the hydrogel	1687:1698	Incorporation of FGF-18 in the hydrogel increased the endothelial cell migration.
29143524	4	45	theme	lactide-co-glycolide	848:867	arg1	hydrogel					887:894	a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel	834:894	a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel by regeneration chemistry	834:920	We developed a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel by regeneration chemistry and then incorporated CaSO4 and FGF-18 for this purpose.
29143524	2	46	theme	accepted	384:391	arg1	use					428:430	the use	424:430	the use of recombinant human bone morphogenetic protein-2 (BMP-2)	424:488	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	2	46	theme	accepted	384:391	arg1	method					393:398	The most accepted method	375:398	The most accepted method	375:398	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	0	47	theme	Bone	82:85	arg1	Regeneration					87:98	Bone Regeneration	82:98	Bone Regeneration	82:98	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model.
29143524	1	48	theme	deeper	359:364	arg1	tissues					366:372	deeper tissues	359:372	deeper tissues	359:372	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	10	49	theme	endothelial	1714:1724	arg1	migration					1731:1739	the endothelial cell migration	1710:1739	the endothelial cell migration	1710:1739	Incorporation of FGF-18 in the hydrogel increased the endothelial cell migration.
29143524	1	50	theme	defect	273:278	arg1	adaptability					287:298	their improved defect margin adaptability	258:298	their improved defect margin adaptability	258:298	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	9	51	theme	red	1644:1646	arg1	staining					1650:1657	alizarin red S staining	1635:1657	alizarin red S staining	1635:1657	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	6	52	theme	fluid	1169:1173	arg1	nature					1175:1180	Shear-thinning Herschel-Bulkley fluid nature	1137:1180	Shear-thinning Herschel-Bulkley fluid nature	1137:1180	Shear-thinning Herschel-Bulkley fluid nature was observed for both hydrogels.
29143524	1	53	theme	adaptability	287:298	arg1	scaffolds					237:245	conventional scaffolds	224:245	conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues	224:372	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	6	54	theme	Shear-thinning	1137:1150	arg1	nature					1175:1180	Shear-thinning Herschel-Bulkley fluid nature	1137:1180	Shear-thinning Herschel-Bulkley fluid nature	1137:1180	Shear-thinning Herschel-Bulkley fluid nature was observed for both hydrogels.
29143524	2	55	from	cost-efficiency	566:580	arg1	doses					604:608	supraphysiological doses	585:608	supraphysiological doses	585:608	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	5	56	from	increase	1003:1010	arg1	modulus					1027:1033	the elastic modulus	1015:1033	the elastic modulus	1015:1033	Rheologically, a 7-fold increase in the elastic modulus was observed in the CaSO4-incorporated chitin-PLGA hydrogels as compared to the chitin-PLGA hydrogel.
29143524	1	57	theme	easier	301:306	arg1	handling					308:315	easier handling	301:315	easier handling	301:315	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	4	58	theme	composite	877:885	arg1	hydrogel					887:894	a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel	834:894	a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel by regeneration chemistry	834:920	We developed a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel by regeneration chemistry and then incorporated CaSO4 and FGF-18 for this purpose.
29143524	3	59	theme	fibroblast	715:724	arg1	FGF-18					744:749	FGF-18	744:749	FGF-18	744:749	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
29143524	3	59	theme	fibroblast	715:724	arg1	factor-18					733:741	fibroblast growth factor-18	715:741	fibroblast growth factor-18 (FGF-18)	715:750	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
29143524	3	59	theme	fibroblast	715:724	arg1	molecule					684:691	a molecule	682:691	a molecule which induces this	682:710	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
29143524	9	60	theme	genes	1510:1514	arg1	staining					1598:1605	immunofluorescence staining	1579:1605	immunofluorescence staining of BMP-2, OCN, and OPN	1579:1628	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	60	theme	genes	1510:1514	arg1	expression					1485:1494	the expression	1481:1494	the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)]	1481:1576	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	60	theme	genes	1510:1514	arg1	staining					1650:1657	alizarin red S staining	1635:1657	alizarin red S staining	1635:1657	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	1	61	theme	conventional	224:235	arg1	scaffolds					237:245	conventional scaffolds	224:245	conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues	224:372	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	4	62	theme	PLGA	871:874	arg1	hydrogel					887:894	a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel	834:894	a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel by regeneration chemistry	834:920	We developed a chitin-poly(lactide-co-glycolide) (PLGA) composite hydrogel by regeneration chemistry and then incorporated CaSO4 and FGF-18 for this purpose.
29143524	8	63	theme	alkaline	1328:1335	arg1	ALP					1350:1352	ALP	1350:1352	ALP	1350:1352	In vitro osteogenic differentiation showed an enhanced alkaline phosphatase (ALP) expression in the FGF-18-containing chitin-PLGA/CaSO4 gel when compared to cells alone.
29143524	8	63	theme	alkaline	1328:1335	arg1	phosphatase					1337:1347	alkaline phosphatase	1328:1347	an enhanced alkaline phosphatase (ALP) expression in the FGF-18-containing chitin-PLGA/CaSO4 gel	1316:1411	In vitro osteogenic differentiation showed an enhanced alkaline phosphatase (ALP) expression in the FGF-18-containing chitin-PLGA/CaSO4 gel when compared to cells alone.
29143524	12	64	theme	sham	2026:2029	arg1	systems					2039:2045	sham control systems	2026:2045	sham control systems	2026:2045	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	3	65	theme	supressing	802:811	arg1	noggin					813:818	supressing noggin	802:818	supressing noggin	802:818	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
29143524	5	66	theme	chitin-PLGA	1115:1125	arg1	hydrogel					1127:1134	the chitin-PLGA hydrogel	1111:1134	the chitin-PLGA hydrogel	1111:1134	Rheologically, a 7-fold increase in the elastic modulus was observed in the CaSO4-incorporated chitin-PLGA hydrogels as compared to the chitin-PLGA hydrogel.
29143524	1	67	theme	craniofacial	139:150	arg1	regeneration					157:168	craniofacial bone regeneration	139:168	craniofacial bone regeneration	139:168	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	9	68	dep	genes	1510:1514	arg1	OPN					1572:1574	OPN	1572:1574	OPN	1572:1574	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	68	dep	genes	1510:1514	arg1	ALP					1524:1526	ALP	1524:1526	ALP	1524:1526	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	68	dep	genes	1510:1514	arg1	BMP-2					1529:1533	BMP-2	1529:1533	BMP-2	1529:1533	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	68	dep	genes	1510:1514	arg1	osteopontin					1559:1569	osteopontin	1559:1569	osteopontin (OPN)	1559:1575	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	68	dep	genes	1510:1514	arg1	genes					1510:1514	osteogenic genes	1499:1514	osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)]	1499:1576	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	68	dep	genes	1510:1514	arg1	[RUNX2					1516:1521	[RUNX2	1516:1521	[RUNX2	1516:1521	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	68	dep	genes	1510:1514	arg1	osteocalcin					1536:1546	osteocalcin	1536:1546	osteocalcin (OCN)	1536:1552	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	68	dep	genes	1510:1514	arg1	OCN					1549:1551	OCN	1549:1551	OCN	1549:1551	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	11	69	theme	live	1841:1844	arg1	μ-CT					1853:1856	longitudinal live animal μ-CT	1828:1856	longitudinal live animal μ-CT	1828:1856	Further, the regeneration potential of the prepared hydrogels was tested in vivo, and longitudinal live animal μ-CT was performed.
29143524	5	70	theme	chitin-PLGA	1074:1084	arg1	hydrogels					1086:1094	the CaSO4-incorporated chitin-PLGA hydrogels	1051:1094	the CaSO4-incorporated chitin-PLGA hydrogels	1051:1094	Rheologically, a 7-fold increase in the elastic modulus was observed in the CaSO4-incorporated chitin-PLGA hydrogels as compared to the chitin-PLGA hydrogel.
29143524	3	71	theme	BMP-2	658:662	arg1	desirable					667:675	desirable	667:675	desirable	667:675	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
29143524	3	71	theme	BMP-2	658:662	arg1	synthesis					645:653	The endogenous synthesis	630:653	The endogenous synthesis of BMP-2	630:662	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
29143524	2	72	theme	supraphysiological	585:602	arg1	doses					604:608	supraphysiological doses	585:608	supraphysiological doses	585:608	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	8	73	theme	FGF-18-containing	1373:1389	arg1	gel					1409:1411	the FGF-18-containing chitin-PLGA/CaSO4 gel	1369:1411	the FGF-18-containing chitin-PLGA/CaSO4 gel	1369:1411	In vitro osteogenic differentiation showed an enhanced alkaline phosphatase (ALP) expression in the FGF-18-containing chitin-PLGA/CaSO4 gel when compared to cells alone.
29143524	0	74	theme	Mice	103:106	arg1	Model					128:132	Mice Cranial Bone Defect Model	103:132	Mice Cranial Bone Defect Model	103:132	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model.
29143524	13	75	theme	FGF-18-incorporated	2151:2169	arg1	candidate					2195:2203	a potential candidate	2183:2203	a potential candidate for craniofacial bone defect regeneration	2183:2245	This shows that the CaSO4 and FGF-18-incorporated hydrogel is a potential candidate for craniofacial bone defect regeneration.
29143524	13	75	theme	FGF-18-incorporated	2151:2169	arg1	CaSO4					2141:2145	CaSO4	2141:2145	CaSO4	2141:2145	This shows that the CaSO4 and FGF-18-incorporated hydrogel is a potential candidate for craniofacial bone defect regeneration.
29143524	13	75	theme	FGF-18-incorporated	2151:2169	arg1	hydrogel					2171:2178	FGF-18-incorporated hydrogel	2151:2178	FGF-18-incorporated hydrogel	2151:2178	This shows that the CaSO4 and FGF-18-incorporated hydrogel is a potential candidate for craniofacial bone defect regeneration.
29143524	1	76	theme	shear-thinning	171:184	arg1	hydrogels					197:205	shear-thinning injectable hydrogels	171:205	shear-thinning injectable hydrogels	171:205	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	5	77	located	observed	1039:1046	arg1	hydrogels					1086:1094	the CaSO4-incorporated chitin-PLGA hydrogels	1051:1094	the CaSO4-incorporated chitin-PLGA hydrogels	1051:1094	Rheologically, a 7-fold increase in the elastic modulus was observed in the CaSO4-incorporated chitin-PLGA hydrogels as compared to the chitin-PLGA hydrogel.
29143524	5	77	located	observed	1039:1046	arg2	increase					1003:1010	a 7-fold increase	994:1010	a 7-fold increase in the elastic modulus	994:1033	Rheologically, a 7-fold increase in the elastic modulus was observed in the CaSO4-incorporated chitin-PLGA hydrogels as compared to the chitin-PLGA hydrogel.
29143524	0	78	theme	Bone	116:119	arg1	Model					128:132	Mice Cranial Bone Defect Model	103:132	Mice Cranial Bone Defect Model	103:132	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model.
29143524	12	79	theme	tetrachrome	2098:2108	arg1	stainings					2110:2118	osteoid tetrachrome stainings	2090:2118	osteoid tetrachrome stainings	2090:2118	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	7	80	theme	FGF-18	1265:1270	arg1	release					1254:1260	sustained release	1244:1260	sustained release of FGF-18	1244:1270	Chitin-PLGA/CaSO4 gel showed sustained release of FGF-18.
29143524	11	81	theme	hydrogels	1794:1802	arg1	potential					1768:1776	the regeneration potential	1751:1776	the regeneration potential of the prepared hydrogels	1751:1802	Further, the regeneration potential of the prepared hydrogels was tested in vivo, and longitudinal live animal μ-CT was performed.
29143524	12	82	theme	complete	1929:1936	arg1	healing					1943:1949	early and almost complete bone healing	1912:1949	early and almost complete bone healing	1912:1949	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	5	83	theme	7-fold	996:1001	arg1	increase					1003:1010	a 7-fold increase	994:1010	a 7-fold increase in the elastic modulus	994:1033	Rheologically, a 7-fold increase in the elastic modulus was observed in the CaSO4-incorporated chitin-PLGA hydrogels as compared to the chitin-PLGA hydrogel.
29143524	0	84	theme	Shear-Thinning	11:24	arg1	Hydrogel					64:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel	0:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel	0:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model.
29143524	9	85	theme	BMP-2	1610:1614	arg1	staining					1598:1605	immunofluorescence staining	1579:1605	immunofluorescence staining of BMP-2, OCN, and OPN	1579:1628	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	85	theme	BMP-2	1610:1614	arg1	expression					1485:1494	the expression	1481:1494	the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)]	1481:1576	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	85	theme	BMP-2	1610:1614	arg1	staining					1650:1657	alizarin red S staining	1635:1657	alizarin red S staining	1635:1657	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	0	86	theme	Chitin-PLGA	52:62	arg1	Hydrogel					64:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel	0:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel	0:71	Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model.
29143524	5	87	theme	elastic	1019:1025	arg1	modulus					1027:1033	the elastic modulus	1015:1033	the elastic modulus	1015:1033	Rheologically, a 7-fold increase in the elastic modulus was observed in the CaSO4-incorporated chitin-PLGA hydrogels as compared to the chitin-PLGA hydrogel.
29143524	9	88	theme	OCN	1617:1619	arg1	staining					1598:1605	immunofluorescence staining	1579:1605	immunofluorescence staining of BMP-2, OCN, and OPN	1579:1628	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	88	theme	OCN	1617:1619	arg1	expression					1485:1494	the expression	1481:1494	the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)]	1481:1576	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	9	88	theme	OCN	1617:1619	arg1	staining					1650:1657	alizarin red S staining	1635:1657	alizarin red S staining	1635:1657	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	2	89	theme	morphogenetic	458:470	arg1	BMP-2					483:487	BMP-2	483:487	BMP-2	483:487	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	2	89	theme	morphogenetic	458:470	arg1	protein-2					472:480	recombinant human bone morphogenetic protein-2	435:480	recombinant human bone morphogenetic protein-2 (BMP-2)	435:488	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	2	90	theme	human	447:451	arg1	BMP-2					483:487	BMP-2	483:487	BMP-2	483:487	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	2	90	theme	human	447:451	arg1	protein-2					472:480	recombinant human bone morphogenetic protein-2	435:480	recombinant human bone morphogenetic protein-2 (BMP-2)	435:488	The most accepted method, after autografting, is the use of recombinant human bone morphogenetic protein-2 (BMP-2); however, complications such as interindividual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported.
29143524	10	91	theme	cell	1726:1729	arg1	migration					1731:1739	the endothelial cell migration	1710:1739	the endothelial cell migration	1710:1739	Incorporation of FGF-18 in the hydrogel increased the endothelial cell migration.
29143524	12	92	theme	FGF-18-loaded	1873:1885	arg1	chitin-PLGA/CaSO4					1887:1903	FGF-18-loaded chitin-PLGA/CaSO4	1873:1903	FGF-18-loaded chitin-PLGA/CaSO4	1873:1903	FGF-18-loaded chitin-PLGA/CaSO4 showed early and almost complete bone healing in comparison with chitin-PLGA/CaSO4, chitin-PLGA/FGF-18, chitin-PLGA, and sham control systems, as confirmed by hematoxylin and eosin and osteoid tetrachrome stainings.
29143524	9	93	theme	alizarin	1635:1642	arg1	staining					1650:1657	alizarin red S staining	1635:1657	alizarin red S staining	1635:1657	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	3	94	theme	endogenous	634:643	arg1	desirable					667:675	desirable	667:675	desirable	667:675	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
29143524	3	94	theme	endogenous	634:643	arg1	synthesis					645:653	The endogenous synthesis	630:653	The endogenous synthesis of BMP-2	630:662	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
29143524	1	95	theme	margin	280:285	arg1	adaptability					287:298	their improved defect margin adaptability	258:298	their improved defect margin adaptability	258:298	For craniofacial bone regeneration, shear-thinning injectable hydrogels are favored over conventional scaffolds because of their improved defect margin adaptability, easier handling, and ability to be injected manually into deeper tissues.
29143524	9	96	theme	S	1648:1648	arg1	staining					1650:1657	alizarin red S staining	1635:1657	alizarin red S staining	1635:1657	Further, it was confirmed by studying the expression of osteogenic genes [RUNX2, ALP, BMP-2, osteocalcin (OCN), and osteopontin (OPN)], immunofluorescence staining of BMP-2, OCN, and OPN, and alizarin red S staining.
29143524	6	97	theme	Herschel-Bulkley	1152:1167	arg1	nature					1175:1180	Shear-thinning Herschel-Bulkley fluid nature	1137:1180	Shear-thinning Herschel-Bulkley fluid nature	1137:1180	Shear-thinning Herschel-Bulkley fluid nature was observed for both hydrogels.
29143524	3	98	theme	growth	726:731	arg1	FGF-18					744:749	FGF-18	744:749	FGF-18	744:749	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
29143524	3	98	theme	growth	726:731	arg1	factor-18					733:741	fibroblast growth factor-18	715:741	fibroblast growth factor-18 (FGF-18)	715:750	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
29143524	3	98	theme	growth	726:731	arg1	molecule					684:691	a molecule	682:691	a molecule which induces this	682:710	The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is fibroblast growth factor-18 (FGF-18) because it can upregulate the BMP-2 expression by supressing noggin.
27419629	4	0	theme	associated	920:929	arg1	integrins					931:939	associated integrins	920:939	associated integrins	920:939	Loss of motility relies on the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness on reduced protease expression.
27419629	2	1	theme	CD44v6	549:554	arg1	CD44v6kd					567:574	CD44v6kd	567:574	CD44v6kd	567:574	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	1	theme	CD44v6	549:554	arg1	knockdown					556:564	a CD44v6 knockdown	547:564	a CD44v6 knockdown (CD44v6kd)	547:575	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	8	2	theme	tumor	1572:1576	arg1	progression					1578:1588	tumor progression	1572:1588	tumor progression	1572:1588	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	2	3	theme	marker	327:332	arg1	isoform					347:353	The CIC marker CD44 variant isoform v6	319:356	The CIC marker CD44 variant isoform v6 (CD44v6)	319:365	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	3	theme	marker	327:332	arg1	CD44v6					359:364	CD44v6	359:364	CD44v6	359:364	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	8	4	theme	progression	1578:1588	arg1	loss					1564:1567	the striking loss	1551:1567	the striking loss of tumor progression by a CD44v6kd	1551:1602	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	0	5	theme	marker	86:91	arg1	expression					93:102	cancer-initiating cell marker expression	63:102	cancer-initiating cell marker expression	63:102	CD44v6-competent tumor exosomes promote motility, invasion and cancer-initiating cell marker expression in pancreatic and colorectal cancer cells.
27419629	8	6	theme	additional	1712:1721	arg1	expression					1734:1743	additional CIC marker expression	1712:1743	additional CIC marker expression	1712:1743	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	2	7	theme	CIC	323:325	arg1	isoform					347:353	The CIC marker CD44 variant isoform v6	319:356	The CIC marker CD44 variant isoform v6 (CD44v6)	319:365	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	7	theme	CIC	323:325	arg1	CD44v6					359:364	CD44v6	359:364	CD44v6	359:364	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	0	8	theme	pancreatic	107:116	arg1	cells					140:144	pancreatic and colorectal cancer cells	107:144	cells	140:144	CD44v6-competent tumor exosomes promote motility, invasion and cancer-initiating cell marker expression in pancreatic and colorectal cancer cells.
27419629	8	9	theme	expression	1734:1743	arg1	integrins					1667:1675	integrins	1667:1675	integrins	1667:1675	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	8	9	theme	expression	1734:1743	arg1	proteases					1681:1689	proteases	1681:1689	proteases	1681:1689	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	8	9	theme	expression	1734:1743	arg1	promotion					1699:1707	its promotion	1695:1707	its promotion of additional CIC marker expression	1695:1743	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	2	10	dep	contribution	432:443	arg1	tranfer					486:492	the tranfer	482:492	the tranfer of migratory and invasive capacity to Non-CIC	482:538	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	11	theme	model.A	588:594	arg1	CD44v6kd					596:603	Non-CIC model.A CD44v6kd	580:603	Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8	580:771	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	6	12	theme	CD44v6kd	1121:1128	arg1	TEX					1130:1132	CD44v6kd TEX	1121:1132	CD44v6kd TEX	1121:1132	As a consequence, unlike the CIC-TEX, CD44v6kd TEX were not taken up by CD44v6kd cells and CIC.
27419629	6	12	theme	CD44v6kd	1121:1128	arg1	consequence					1088:1098	a consequence	1086:1098	a consequence	1086:1098	As a consequence, unlike the CIC-TEX, CD44v6kd TEX were not taken up by CD44v6kd cells and CIC.
27419629	3	13	theme	CD44v6-promoted	802:816	arg1	motility					818:825	CD44v6-promoted motility	802:825	CD44v6-promoted motility	802:825	This aggravated the loss of CD44v6-promoted motility and invasion.
27419629	4	14	theme	distorted	872:880	arg1	cooperation					882:892	the distorted cooperation	868:892	the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness	868:964	Loss of motility relies on the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness on reduced protease expression.
27419629	8	15	theme	direct	1441:1446	arg1	impact					1448:1453	a direct impact	1439:1453	a direct impact of CIC-TEX on the host	1439:1476	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	2	16	theme	Non-CIC	580:586	arg1	CD44v6kd					596:603	Non-CIC model.A CD44v6kd	580:603	Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8	580:771	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	0	17	theme	colorectal	122:131	arg1	cells					140:144	pancreatic and colorectal cancer cells	107:144	cells	140:144	CD44v6-competent tumor exosomes promote motility, invasion and cancer-initiating cell marker expression in pancreatic and colorectal cancer cells.
27419629	0	18	from	invasion	50:57	arg1	cells					140:144	pancreatic and colorectal cancer cells	107:144	cells	140:144	CD44v6-competent tumor exosomes promote motility, invasion and cancer-initiating cell marker expression in pancreatic and colorectal cancer cells.
27419629	2	19	theme	migratory	497:505	arg1	capacity					520:527	migratory and invasive capacity	497:527	migratory and invasive capacity	497:527	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	8	20	theme	CIC	1723:1725	arg1	expression					1734:1743	additional CIC marker expression	1712:1743	additional CIC marker expression	1712:1743	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	7	21	theme	metastatic	1335:1344	arg1	competence					1346:1355	migratory, invasive and metastatic competence	1311:1355	migratory, invasive and metastatic competence	1311:1355	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	7	22	from	expression	1266:1275	arg1	cells					1289:1293	CD44v6kd cells	1280:1293	CD44v6kd cells	1280:1293	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	4	23	theme	motility	849:856	arg1	Loss					841:844	Loss	841:844	Loss of motility	841:856	Loss of motility relies on the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness on reduced protease expression.
27419629	2	24	from	contribution	432:443	arg1	cells					422:426	cells	422:426	cells	422:426	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	4	25	theme	invasiveness	953:964	arg1	loss					945:948	loss	945:948	loss of invasiveness	945:964	Loss of motility relies on the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness on reduced protease expression.
27419629	4	25	theme	invasiveness	953:964	arg1	integrins					931:939	associated integrins	920:939	associated integrins	920:939	Loss of motility relies on the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness on reduced protease expression.
27419629	3	26	theme	invasion	831:838	arg1	loss					794:797	the loss	790:797	the loss of CD44v6-promoted motility and invasion	790:838	This aggravated the loss of CD44v6-promoted motility and invasion.
27419629	4	27	theme	CD44v6	897:902	arg1	cooperation					882:892	the distorted cooperation	868:892	the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness	868:964	Loss of motility relies on the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness on reduced protease expression.
27419629	0	28	theme	tumor	17:21	arg1	exosomes					23:30	CD44v6-competent tumor exosomes	0:30	CD44v6-competent tumor exosomes	0:30	CD44v6-competent tumor exosomes promote motility, invasion and cancer-initiating cell marker expression in pancreatic and colorectal cancer cells.
27419629	0	29	from	motility	40:47	arg1	cells					140:144	pancreatic and colorectal cancer cells	107:144	cells	140:144	CD44v6-competent tumor exosomes promote motility, invasion and cancer-initiating cell marker expression in pancreatic and colorectal cancer cells.
27419629	4	30	theme	Tspan8	908:913	arg1	cooperation					882:892	the distorted cooperation	868:892	the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness	868:964	Loss of motility relies on the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness on reduced protease expression.
27419629	7	31	theme	invasive	1322:1329	arg1	competence					1346:1355	migratory, invasive and metastatic competence	1311:1355	migratory, invasive and metastatic competence	1311:1355	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	1	32	theme	metastatic	189:198	arg1	spread					200:205	metastatic spread	189:205	metastatic spread	189:205	Cancer-initiating cells (CIC) account for metastatic spread, which may rely mostly on CIC exosomes (TEX) that affect host cells and can transfer CIC features into Non-CIC.
27419629	0	33	theme	CD44v6-competent	0:15	arg1	exosomes					23:30	CD44v6-competent tumor exosomes	0:30	CD44v6-competent tumor exosomes	0:30	CD44v6-competent tumor exosomes promote motility, invasion and cancer-initiating cell marker expression in pancreatic and colorectal cancer cells.
27419629	8	34	theme	myeloid	1410:1416	arg1	cells					1418:1422	myeloid cells	1410:1422	myeloid cells	1410:1422	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	8	35	theme	CD44v6kd	1520:1527	arg1	cells.Taken					1529:1539	reprogrammed CD44v6kd cells.Taken	1507:1539	reprogrammed CD44v6kd cells.Taken	1507:1539	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	7	36	from	cells	1289:1293	arg1	correction					1228:1237	partial correction	1220:1237	partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence	1220:1355	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	7	37	theme	CIC	1242:1244	arg1	marker					1246:1251	CIC marker and protease expression	1242:1275	marker	1246:1251	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	4	38	theme	protease	977:984	arg1	expression					986:995	reduced protease expression	969:995	reduced protease expression	969:995	Loss of motility relies on the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness on reduced protease expression.
27419629	1	39	theme	host	264:267	arg1	cells					269:273	host cells	264:273	host cells	264:273	Cancer-initiating cells (CIC) account for metastatic spread, which may rely mostly on CIC exosomes (TEX) that affect host cells and can transfer CIC features into Non-CIC.
27419629	7	40	theme	migratory	1311:1319	arg1	competence					1346:1355	migratory, invasive and metastatic competence	1311:1355	migratory, invasive and metastatic competence	1311:1355	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	7	41	theme	partial	1220:1226	arg1	correction					1228:1237	partial correction	1220:1237	partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence	1220:1355	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	4	42	theme	reduced	969:975	arg1	expression					986:995	reduced protease expression	969:995	reduced protease expression	969:995	Loss of motility relies on the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness on reduced protease expression.
27419629	0	43	theme	cancer	133:138	arg1	cells					140:144	pancreatic and colorectal cancer cells	107:144	cells	140:144	CD44v6-competent tumor exosomes promote motility, invasion and cancer-initiating cell marker expression in pancreatic and colorectal cancer cells.
27419629	0	44	from	expression	93:102	arg1	cells					140:144	pancreatic and colorectal cancer cells	107:144	cells	140:144	CD44v6-competent tumor exosomes promote motility, invasion and cancer-initiating cell marker expression in pancreatic and colorectal cancer cells.
27419629	2	45	theme	characteristics	685:699	arg1	loss					673:676	loss	673:676	loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8	673:771	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	8	46	theme	CD44v6	1630:1635	arg1	capacity					1618:1625	the capacity	1614:1625	the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression	1614:1743	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	7	47	from	correction	1228:1237	arg1	cells					1289:1293	CD44v6kd cells	1280:1293	CD44v6kd cells	1280:1293	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	8	48	theme	striking	1555:1562	arg1	loss					1564:1567	the striking loss	1551:1567	the striking loss of tumor progression by a CD44v6kd	1551:1602	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	2	49	theme	CIC	681:683	arg1	characteristics					685:699	CIC characteristics	681:699	CIC characteristics including downregulation of additional CIC markers, particularly Tspan8	681:771	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	49	theme	CIC	681:683	arg1	downregulation					711:724	downregulation	711:724	downregulation of additional CIC markers, particularly Tspan8	711:771	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	7	50	theme	marker	1246:1251	arg1	correction					1228:1237	partial correction	1220:1237	partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence	1220:1355	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	8	51	from	impact	1448:1453	arg1	host					1473:1476	the host	1469:1476	the host	1469:1476	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	6	52	theme	CD44v6kd	1155:1162	arg1	cells					1164:1168	CD44v6kd cells	1155:1168	CD44v6kd cells	1155:1168	As a consequence, unlike the CIC-TEX, CD44v6kd TEX were not taken up by CD44v6kd cells and CIC.
27419629	8	53	theme	associating	1655:1665	arg1	integrins					1667:1675	integrins	1667:1675	integrins	1667:1675	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	8	54	theme	CIC-TEX	1458:1464	arg1	impact					1448:1453	a direct impact	1439:1453	a direct impact of CIC-TEX on the host	1439:1476	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	7	55	theme	CIC-TEX	1193:1199	arg1	uptake					1183:1188	The uptake	1179:1188	The uptake of CIC-TEX	1179:1199	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	8	56	theme	due	1432:1434	arg1	cells					1418:1422	myeloid cells	1410:1422	myeloid cells	1410:1422	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	1	57	theme	CIC	292:294	arg1	features					296:303	CIC features	292:303	CIC features	292:303	Cancer-initiating cells (CIC) account for metastatic spread, which may rely mostly on CIC exosomes (TEX) that affect host cells and can transfer CIC features into Non-CIC.
27419629	2	58	theme	markers	744:750	arg1	downregulation					711:724	downregulation	711:724	downregulation of additional CIC markers, particularly Tspan8	711:771	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	59	theme	capacity	520:527	arg1	tranfer					486:492	the tranfer	482:492	the tranfer of migratory and invasive capacity to Non-CIC	482:538	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	60	theme	colorectal	629:638	arg1	CoCa					654:657	CoCa	654:657	CoCa	654:657	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	60	theme	colorectal	629:638	arg1	cancer					640:645	colorectal cancer	629:645	colorectal cancer (PaCa, CoCa)	629:658	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	61	from	CD44v6kd	596:603	arg1	CoCa					654:657	CoCa	654:657	CoCa	654:657	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	61	from	CD44v6kd	596:603	arg1	pancreatic					614:623	pancreatic	614:623	pancreatic	614:623	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	61	from	CD44v6kd	596:603	arg1	cancer					640:645	colorectal cancer	629:645	colorectal cancer (PaCa, CoCa)	629:658	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	62	theme	CIC	740:742	arg1	markers					744:750	additional CIC markers	729:750	additional CIC markers	729:750	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	62	theme	CIC	740:742	arg1	Tspan8					766:771	Tspan8	766:771	Tspan8	766:771	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	8	63	theme	marker	1727:1732	arg1	expression					1734:1743	additional CIC marker expression	1712:1743	additional CIC marker expression	1712:1743	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	2	64	theme	invasive	511:518	arg1	capacity					520:527	migratory and invasive capacity	497:527	migratory and invasive capacity	497:527	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	8	65	theme	cells	1418:1422	arg1	angiogenesis					1380:1391	angiogenesis	1380:1391	angiogenesis	1380:1391	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	8	65	theme	cells	1418:1422	arg1	expansion					1397:1405	expansion	1397:1405	expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken	1397:1539	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
27419629	7	66	theme	CD44v6kd	1280:1287	arg1	cells					1289:1293	CD44v6kd cells	1280:1293	CD44v6kd cells	1280:1293	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	1	67	theme	Cancer-initiating	147:163	arg1	CIC					172:174	CIC	172:174	CIC	172:174	Cancer-initiating cells (CIC) account for metastatic spread, which may rely mostly on CIC exosomes (TEX) that affect host cells and can transfer CIC features into Non-CIC.
27419629	1	67	theme	Cancer-initiating	147:163	arg1	cells					165:169	Cancer-initiating cells	147:169	Cancer-initiating cells (CIC)	147:175	Cancer-initiating cells (CIC) account for metastatic spread, which may rely mostly on CIC exosomes (TEX) that affect host cells and can transfer CIC features into Non-CIC.
27419629	2	68	theme	additional	729:738	arg1	markers					744:750	additional CIC markers	729:750	additional CIC markers	729:750	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	68	theme	additional	729:738	arg1	Tspan8					766:771	Tspan8	766:771	Tspan8	766:771	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	9	69	theme	CIC-TEX	1803:1809	arg1	uptake					1811:1816	CIC-TEX uptake	1803:1816	CIC-TEX uptake	1803:1816	The defects by a CD44v6kd are efficiently corrected upon CIC-TEX uptake.
27419629	0	70	theme	cell	81:84	arg1	expression					93:102	cancer-initiating cell marker expression	63:102	cancer-initiating cell marker expression	63:102	CD44v6-competent tumor exosomes promote motility, invasion and cancer-initiating cell marker expression in pancreatic and colorectal cancer cells.
27419629	2	71	from	TEX	478:480	arg1	cells					422:426	cells	422:426	cells	422:426	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	7	72	theme	protease	1257:1264	arg1	expression					1266:1275	CIC marker and protease expression	1242:1275	expression	1266:1275	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	7	73	from	marker	1246:1251	arg1	cells					1289:1293	CD44v6kd cells	1280:1293	CD44v6kd cells	1280:1293	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	2	74	theme	variant	339:345	arg1	isoform					347:353	The CIC marker CD44 variant isoform v6	319:356	The CIC marker CD44 variant isoform v6 (CD44v6)	319:365	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	74	theme	variant	339:345	arg1	CD44v6					359:364	CD44v6	359:364	CD44v6	359:364	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	75	dep	pancreatic	614:623	arg1	lines					660:664	lines	660:664	lines	660:664	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	1	76	theme	CIC	233:235	arg1	TEX					247:249	TEX	247:249	TEX	247:249	Cancer-initiating cells (CIC) account for metastatic spread, which may rely mostly on CIC exosomes (TEX) that affect host cells and can transfer CIC features into Non-CIC.
27419629	1	76	theme	CIC	233:235	arg1	exosomes					237:244	CIC exosomes	233:244	CIC exosomes (TEX) that affect host cells and can transfer CIC features into Non-CIC	233:316	Cancer-initiating cells (CIC) account for metastatic spread, which may rely mostly on CIC exosomes (TEX) that affect host cells and can transfer CIC features into Non-CIC.
27419629	0	77	theme	cancer-initiating	63:79	arg1	expression					93:102	cancer-initiating cell marker expression	63:102	cancer-initiating cell marker expression	63:102	CD44v6-competent tumor exosomes promote motility, invasion and cancer-initiating cell marker expression in pancreatic and colorectal cancer cells.
27419629	5	78	theme	CD44v6kd-TEX	1057:1068	arg1	composition					1070:1080	the CD44v6kd-TEX composition	1053:1080	the CD44v6kd-TEX composition	1053:1080	These deficits, transferred into TEX, severely altered the CD44v6kd-TEX composition.
27419629	7	79	theme	expression	1266:1275	arg1	correction					1228:1237	partial correction	1220:1237	partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence	1220:1355	The uptake of CIC-TEX was accompanied by partial correction of CIC marker and protease expression in CD44v6kd cells, which regained migratory, invasive and metastatic competence.
27419629	4	80	with	cooperation	882:892	arg1	loss					945:948	loss	945:948	loss of invasiveness	945:964	Loss of motility relies on the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness on reduced protease expression.
27419629	4	80	with	cooperation	882:892	arg1	integrins					931:939	associated integrins	920:939	associated integrins	920:939	Loss of motility relies on the distorted cooperation of CD44v6 and Tspan8 with associated integrins and loss of invasiveness on reduced protease expression.
27419629	2	81	theme	CD44	334:337	arg1	isoform					347:353	The CIC marker CD44 variant isoform v6	319:356	The CIC marker CD44 variant isoform v6 (CD44v6)	319:365	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	2	81	theme	CD44	334:337	arg1	CD44v6					359:364	CD44v6	359:364	CD44v6	359:364	The CIC marker CD44 variant isoform v6 (CD44v6) being known for metastasis-promotion, we elaborated in cells its contribution to migration and invasion and in TEX the tranfer of migratory and invasive capacity to Non-CIC, using a CD44v6 knockdown (CD44v6kd) as Non-CIC model.A CD44v6kd in human pancreatic and colorectal cancer (PaCa, CoCa) lines led to loss of CIC characteristics including downregulation of additional CIC markers, particularly Tspan8.
27419629	3	82	theme	motility	818:825	arg1	loss					794:797	the loss	790:797	the loss of CD44v6-promoted motility and invasion	790:838	This aggravated the loss of CD44v6-promoted motility and invasion.
27419629	8	83	theme	reprogrammed	1507:1518	arg1	cells.Taken					1529:1539	reprogrammed CD44v6kd cells.Taken	1507:1539	reprogrammed CD44v6kd cells.Taken	1507:1539	CIC-TEX also fostered angiogenesis and expansion of myeloid cells, likely due to a direct impact of CIC-TEX on the host, which could be supported by reprogrammed CD44v6kd cells.Taken together, the striking loss of tumor progression by a CD44v6kd relies on the capacity of CD44v6 to cooperate with associating integrins and proteases and its promotion of additional CIC marker expression.
25869450	6	0	theme	formulated	748:757	arg1	pellets					759:765	formulated pellets	748:765	formulated pellets of the same composition and the innovator Effexor(®)XR pellets	748:828	In an attempt to weigh up mini-tablets versus pellets as multiparticulate carriers, Vx-loaded mini-tablets were compared to formulated pellets of the same composition and the innovator Effexor(®)XR pellets.
25869450	7	1	theme	ethyl	906:910	arg1	film					922:925	the core and ethyl cellulose film	893:925	film	922:925	Formulations were prepared using various polymer hydrogels in the core and ethyl cellulose film coating with increasing thickness.
25869450	11	2	from	cellulose	1206:1214	arg1	core					1223:1226	the core	1219:1226	the core	1219:1226	Ethyl cellulose in the core ensured faster release due to polymer migration to the surface and pore formation in the coat.
25869450	3	3	theme	aforementioned	335:348	arg1	points					350:355	the aforementioned points	331:355	the aforementioned points	331:355	In this study, the aforementioned points were discussed.
25869450	8	4	theme	extended	997:1004	arg1	release					1009:1015	extended Vx release	997:1015	extended Vx release (<60%, 8h)	997:1026	Mini-tablets (diameter 2mm) showed extended Vx release (<60%, 8h).
25869450	13	5	theme	quality	1489:1495	arg1	terms					1457:1461	terms	1457:1461	terms of manufacturing, product quality and economical aspects	1457:1518	Industrially speaking, mini-tablets proved to be superior to pellets in terms of manufacturing, product quality and economical aspects.
25869450	5	6	theme	antidepressant	446:459	arg1	payload					615:621	a challenging payload	601:621	a challenging payload	601:621	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	6	theme	antidepressant	446:459	arg1	Vx					488:489	Vx	488:489	Vx	488:489	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	6	theme	antidepressant	446:459	arg1	drug					510:513	a highly soluble drug	493:513	a highly soluble drug undergoing first pass effect, low bioavailability and short half-life	493:583	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	6	theme	antidepressant	446:459	arg1	hydrochloride					473:485	The antidepressant venlafaxine hydrochloride	442:485	The antidepressant venlafaxine hydrochloride (Vx)	442:490	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	13	7	theme	manufacturing	1466:1478	arg1	terms					1457:1461	terms	1457:1461	terms of manufacturing, product quality and economical aspects	1457:1518	Industrially speaking, mini-tablets proved to be superior to pellets in terms of manufacturing, product quality and economical aspects.
25869450	4	8	theme	controlled	408:417	arg1	delivery					419:426	oral controlled delivery	403:426	oral controlled delivery	403:426	Moreover, their potential for oral controlled delivery was assessed.
25869450	5	9	theme	venlafaxine	461:471	arg1	payload					615:621	a challenging payload	601:621	a challenging payload	601:621	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	9	theme	venlafaxine	461:471	arg1	Vx					488:489	Vx	488:489	Vx	488:489	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	9	theme	venlafaxine	461:471	arg1	drug					510:513	a highly soluble drug	493:513	a highly soluble drug undergoing first pass effect, low bioavailability and short half-life	493:583	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	9	theme	venlafaxine	461:471	arg1	hydrochloride					473:485	The antidepressant venlafaxine hydrochloride	442:485	The antidepressant venlafaxine hydrochloride (Vx)	442:490	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	0	10	theme	soluble	103:109	arg1	drugs					111:115	highly soluble drugs	96:115	highly soluble drugs	96:115	Mini-tablets versus pellets as promising multiparticulate modified release delivery systems for highly soluble drugs.
25869450	11	11	from	formation	1300:1308	arg1	coat					1317:1320	the coat	1313:1320	the coat	1313:1320	Ethyl cellulose in the core ensured faster release due to polymer migration to the surface and pore formation in the coat.
25869450	11	12	theme	polymer	1258:1264	arg1	migration					1266:1274	polymer migration	1258:1274	polymer migration to the surface	1258:1289	Ethyl cellulose in the core ensured faster release due to polymer migration to the surface and pore formation in the coat.
25869450	6	13	theme	XR	819:820	arg1	pellets					822:828	the innovator Effexor(®)XR pellets	795:828	the innovator Effexor(®)XR pellets	795:828	In an attempt to weigh up mini-tablets versus pellets as multiparticulate carriers, Vx-loaded mini-tablets were compared to formulated pellets of the same composition and the innovator Effexor(®)XR pellets.
25869450	7	14	theme	polymer	872:878	arg1	hydrogels					880:888	various polymer hydrogels	864:888	various polymer hydrogels in the core and ethyl cellulose film coating with increasing thickness	864:959	Formulations were prepared using various polymer hydrogels in the core and ethyl cellulose film coating with increasing thickness.
25869450	8	15	theme	Vx	1006:1007	arg1	release					1009:1015	extended Vx release	997:1015	extended Vx release (<60%, 8h)	997:1026	Mini-tablets (diameter 2mm) showed extended Vx release (<60%, 8h).
25869450	1	16	dep	mini-tablets	126:137	arg1	tablets					140:146	tablets	140:146	tablets	140:146	Whether mini-tablets (tablets, diameters ≤6mm) belong to single- or multiple-unit dosage forms is still questionable.
25869450	1	16	dep	mini-tablets	126:137	arg1	diameters					149:157	diameters ≤6mm	149:162	diameters ≤6mm	149:162	Whether mini-tablets (tablets, diameters ≤6mm) belong to single- or multiple-unit dosage forms is still questionable.
25869450	9	17	theme	Effexor	1068:1074	arg1	pellets					1081:1087	Effexor(®)XR pellets	1068:1087	Effexor(®)XR pellets	1068:1087	Indeed, release profiles comparable to Effexor(®)XR pellets were obtained.
25869450	10	18	theme	equivalent	1176:1185	arg1	retardation					1187:1197	equivalent retardation	1176:1197	equivalent retardation	1176:1197	Remarkably higher coating thickness was required for pellets to provide equivalent retardation.
25869450	11	19	theme	pore	1295:1298	arg1	formation					1300:1308	pore formation	1295:1308	pore formation in the coat	1295:1320	Ethyl cellulose in the core ensured faster release due to polymer migration to the surface and pore formation in the coat.
25869450	6	20	theme	®	817:817	arg1	pellets					822:828	the innovator Effexor(®)XR pellets	795:828	the innovator Effexor(®)XR pellets	795:828	In an attempt to weigh up mini-tablets versus pellets as multiparticulate carriers, Vx-loaded mini-tablets were compared to formulated pellets of the same composition and the innovator Effexor(®)XR pellets.
25869450	5	21	theme	first	526:530	arg1	effect					537:542	first pass effect	526:542	first pass effect	526:542	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	21	theme	first	526:530	arg1	bioavailability					549:563	low bioavailability	545:563	low bioavailability	545:563	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	21	theme	first	526:530	arg1	half-life					575:583	short half-life	569:583	short half-life	569:583	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	1	22	theme	single-	175:181	arg1	forms					207:211	single- or multiple-unit dosage forms	175:211	single- or multiple-unit dosage forms	175:211	Whether mini-tablets (tablets, diameters ≤6mm) belong to single- or multiple-unit dosage forms is still questionable.
25869450	14	23	dep	point	1529:1533	arg1	out					1535:1537	out	1535:1537	out	1535:1537	Results point out the urgent need for standardized evaluation procedures for mini-tablets.
25869450	13	24	dep	proved	1421:1426	arg1	speaking					1398:1405	speaking	1398:1405	speaking	1398:1405	Industrially speaking, mini-tablets proved to be superior to pellets in terms of manufacturing, product quality and economical aspects.
25869450	5	25	theme	pass	532:535	arg1	effect					537:542	first pass effect	526:542	first pass effect	526:542	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	25	theme	pass	532:535	arg1	bioavailability					549:563	low bioavailability	545:563	low bioavailability	545:563	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	25	theme	pass	532:535	arg1	half-life					575:583	short half-life	569:583	short half-life	569:583	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	9	26	theme	release	1037:1043	arg1	profiles					1045:1052	release profiles	1037:1052	release profiles comparable to Effexor(®)XR pellets	1037:1087	Indeed, release profiles comparable to Effexor(®)XR pellets were obtained.
25869450	10	27	theme	higher	1115:1120	arg1	thickness					1130:1138	Remarkably higher coating thickness	1104:1138	Remarkably higher coating thickness	1104:1138	Remarkably higher coating thickness was required for pellets to provide equivalent retardation.
25869450	4	28	theme	oral	403:406	arg1	delivery					419:426	oral controlled delivery	403:426	oral controlled delivery	403:426	Moreover, their potential for oral controlled delivery was assessed.
25869450	11	29	theme	Ethyl	1200:1204	arg1	cellulose					1206:1214	Ethyl cellulose	1200:1214	Ethyl cellulose in the core	1200:1226	Ethyl cellulose in the core ensured faster release due to polymer migration to the surface and pore formation in the coat.
25869450	6	30	theme	Vx-loaded	708:716	arg1	mini-tablets					718:729	Vx-loaded mini-tablets	708:729	Vx-loaded mini-tablets	708:729	In an attempt to weigh up mini-tablets versus pellets as multiparticulate carriers, Vx-loaded mini-tablets were compared to formulated pellets of the same composition and the innovator Effexor(®)XR pellets.
25869450	1	31	theme	multiple-unit	186:198	arg1	forms					207:211	single- or multiple-unit dosage forms	175:211	single- or multiple-unit dosage forms	175:211	Whether mini-tablets (tablets, diameters ≤6mm) belong to single- or multiple-unit dosage forms is still questionable.
25869450	6	32	theme	Effexor	809:815	arg1	pellets					822:828	the innovator Effexor(®)XR pellets	795:828	the innovator Effexor(®)XR pellets	795:828	In an attempt to weigh up mini-tablets versus pellets as multiparticulate carriers, Vx-loaded mini-tablets were compared to formulated pellets of the same composition and the innovator Effexor(®)XR pellets.
25869450	5	33	theme	challenging	603:613	arg1	hydrochloride					473:485	The antidepressant venlafaxine hydrochloride	442:485	The antidepressant venlafaxine hydrochloride (Vx)	442:490	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	33	theme	challenging	603:613	arg1	payload					615:621	a challenging payload	601:621	a challenging payload	601:621	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	9	34	theme	XR	1078:1079	arg1	pellets					1081:1087	Effexor(®)XR pellets	1068:1087	Effexor(®)XR pellets	1068:1087	Indeed, release profiles comparable to Effexor(®)XR pellets were obtained.
25869450	1	35	theme	dosage	200:205	arg1	forms					207:211	single- or multiple-unit dosage forms	175:211	single- or multiple-unit dosage forms	175:211	Whether mini-tablets (tablets, diameters ≤6mm) belong to single- or multiple-unit dosage forms is still questionable.
25869450	9	36	theme	®	1076:1076	arg1	pellets					1081:1087	Effexor(®)XR pellets	1068:1087	Effexor(®)XR pellets	1068:1087	Indeed, release profiles comparable to Effexor(®)XR pellets were obtained.
25869450	13	37	from	superior	1434:1441	arg1	terms					1457:1461	terms	1457:1461	terms of manufacturing, product quality and economical aspects	1457:1518	Industrially speaking, mini-tablets proved to be superior to pellets in terms of manufacturing, product quality and economical aspects.
25869450	13	38	from	terms	1457:1461	arg1	superior					1434:1441	superior	1434:1441	superior	1434:1441	Industrially speaking, mini-tablets proved to be superior to pellets in terms of manufacturing, product quality and economical aspects.
25869450	6	39	theme	multiparticulate	681:696	arg1	carriers					698:705	multiparticulate carriers	681:705	multiparticulate carriers	681:705	In an attempt to weigh up mini-tablets versus pellets as multiparticulate carriers, Vx-loaded mini-tablets were compared to formulated pellets of the same composition and the innovator Effexor(®)XR pellets.
25869450	8	40	dep	release	1009:1015	arg1	8h					1024:1025	8h	1024:1025	8h	1024:1025	Mini-tablets (diameter 2mm) showed extended Vx release (<60%, 8h).
25869450	8	40	dep	release	1009:1015	arg1	%					1021:1021	<60%	1018:1021	<60%	1018:1021	Mini-tablets (diameter 2mm) showed extended Vx release (<60%, 8h).
25869450	14	41	theme	evaluation	1572:1581	arg1	procedures					1583:1592	standardized evaluation procedures	1559:1592	standardized evaluation procedures for mini-tablets	1559:1609	Results point out the urgent need for standardized evaluation procedures for mini-tablets.
25869450	5	42	theme	soluble	502:508	arg1	hydrochloride					473:485	The antidepressant venlafaxine hydrochloride	442:485	The antidepressant venlafaxine hydrochloride (Vx)	442:490	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	42	theme	soluble	502:508	arg1	drug					510:513	a highly soluble drug	493:513	a highly soluble drug undergoing first pass effect, low bioavailability and short half-life	493:583	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	0	43	theme	promising	31:39	arg1	Mini-tablets					0:11	Mini-tablets	0:11	Mini-tablets	0:11	Mini-tablets versus pellets as promising multiparticulate modified release delivery systems for highly soluble drugs.
25869450	10	44	theme	coating	1122:1128	arg1	thickness					1130:1138	Remarkably higher coating thickness	1104:1138	Remarkably higher coating thickness	1104:1138	Remarkably higher coating thickness was required for pellets to provide equivalent retardation.
25869450	0	45	theme	release	67:73	arg1	systems					84:90	modified release delivery systems	58:90	modified release delivery systems for highly soluble drugs	58:115	Mini-tablets versus pellets as promising multiparticulate modified release delivery systems for highly soluble drugs.
25869450	7	46	from	hydrogels	880:888	arg1	film					922:925	the core and ethyl cellulose film	893:925	film	922:925	Formulations were prepared using various polymer hydrogels in the core and ethyl cellulose film coating with increasing thickness.
25869450	7	46	from	hydrogels	880:888	arg1	core					897:900	the core and ethyl cellulose film	893:925	core	897:900	Formulations were prepared using various polymer hydrogels in the core and ethyl cellulose film coating with increasing thickness.
25869450	12	47	theme	higher	1343:1348	arg1	stability					1350:1358	higher stability	1343:1358	higher stability	1343:1358	mini-tablets showed higher stability to pellets upon storage.
25869450	5	48	theme	short	569:573	arg1	effect					537:542	first pass effect	526:542	first pass effect	526:542	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	48	theme	short	569:573	arg1	half-life					575:583	short half-life	569:583	short half-life	569:583	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	0	49	theme	modified	58:65	arg1	systems					84:90	modified release delivery systems	58:90	modified release delivery systems for highly soluble drugs	58:115	Mini-tablets versus pellets as promising multiparticulate modified release delivery systems for highly soluble drugs.
25869450	11	50	from	migration	1266:1274	arg1	coat					1317:1320	the coat	1313:1320	the coat	1313:1320	Ethyl cellulose in the core ensured faster release due to polymer migration to the surface and pore formation in the coat.
25869450	6	51	theme	composition	779:789	arg1	pellets					759:765	formulated pellets	748:765	formulated pellets of the same composition and the innovator Effexor(®)XR pellets	748:828	In an attempt to weigh up mini-tablets versus pellets as multiparticulate carriers, Vx-loaded mini-tablets were compared to formulated pellets of the same composition and the innovator Effexor(®)XR pellets.
25869450	6	52	theme	innovator	799:807	arg1	pellets					822:828	the innovator Effexor(®)XR pellets	795:828	the innovator Effexor(®)XR pellets	795:828	In an attempt to weigh up mini-tablets versus pellets as multiparticulate carriers, Vx-loaded mini-tablets were compared to formulated pellets of the same composition and the innovator Effexor(®)XR pellets.
25869450	13	53	theme	economical	1501:1510	arg1	aspects					1512:1518	economical aspects	1501:1518	economical aspects	1501:1518	Industrially speaking, mini-tablets proved to be superior to pellets in terms of manufacturing, product quality and economical aspects.
25869450	6	54	theme	pellets	822:828	arg1	pellets					759:765	formulated pellets	748:765	formulated pellets of the same composition and the innovator Effexor(®)XR pellets	748:828	In an attempt to weigh up mini-tablets versus pellets as multiparticulate carriers, Vx-loaded mini-tablets were compared to formulated pellets of the same composition and the innovator Effexor(®)XR pellets.
25869450	6	55	theme	same	774:777	arg1	composition					779:789	the same composition	770:789	the same composition	770:789	In an attempt to weigh up mini-tablets versus pellets as multiparticulate carriers, Vx-loaded mini-tablets were compared to formulated pellets of the same composition and the innovator Effexor(®)XR pellets.
25869450	13	56	theme	aspects	1512:1518	arg1	terms					1457:1461	terms	1457:1461	terms of manufacturing, product quality and economical aspects	1457:1518	Industrially speaking, mini-tablets proved to be superior to pellets in terms of manufacturing, product quality and economical aspects.
25869450	8	57	theme	diameter	976:983	arg1	Mini-tablets					962:973	Mini-tablets	962:973	Mini-tablets (diameter 2mm)	962:988	Mini-tablets (diameter 2mm) showed extended Vx release (<60%, 8h).
25869450	8	57	theme	diameter	976:983	arg1	2mm					985:987	diameter 2mm	976:987	diameter 2mm	976:987	Mini-tablets (diameter 2mm) showed extended Vx release (<60%, 8h).
25869450	7	58	theme	cellulose	912:920	arg1	film					922:925	the core and ethyl cellulose film	893:925	film	922:925	Formulations were prepared using various polymer hydrogels in the core and ethyl cellulose film coating with increasing thickness.
25869450	7	59	theme	various	864:870	arg1	hydrogels					880:888	various polymer hydrogels	864:888	various polymer hydrogels in the core and ethyl cellulose film coating with increasing thickness	864:959	Formulations were prepared using various polymer hydrogels in the core and ethyl cellulose film coating with increasing thickness.
25869450	9	60	theme	comparable	1054:1063	arg1	profiles					1045:1052	release profiles	1037:1052	release profiles comparable to Effexor(®)XR pellets	1037:1087	Indeed, release profiles comparable to Effexor(®)XR pellets were obtained.
25869450	2	61	theme	evaluation	264:273	arg1	procedures					275:284	Pharmacopoeial evaluation procedures	249:284	Pharmacopoeial evaluation procedures for mini-tablets	249:301	Accordingly, Pharmacopoeial evaluation procedures for mini-tablets are lacking.
25869450	14	62	theme	standardized	1559:1570	arg1	procedures					1583:1592	standardized evaluation procedures	1559:1592	standardized evaluation procedures for mini-tablets	1559:1609	Results point out the urgent need for standardized evaluation procedures for mini-tablets.
25869450	13	63	theme	product	1481:1487	arg1	quality					1489:1495	product quality	1481:1495	product quality	1481:1495	Industrially speaking, mini-tablets proved to be superior to pellets in terms of manufacturing, product quality and economical aspects.
25869450	0	64	theme	delivery	75:82	arg1	systems					84:90	modified release delivery systems	58:90	modified release delivery systems for highly soluble drugs	58:115	Mini-tablets versus pellets as promising multiparticulate modified release delivery systems for highly soluble drugs.
25869450	2	65	theme	Pharmacopoeial	249:262	arg1	procedures					275:284	Pharmacopoeial evaluation procedures	249:284	Pharmacopoeial evaluation procedures for mini-tablets	249:301	Accordingly, Pharmacopoeial evaluation procedures for mini-tablets are lacking.
25869450	5	66	theme	low	545:547	arg1	effect					537:542	first pass effect	526:542	first pass effect	526:542	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	5	66	theme	low	545:547	arg1	bioavailability					549:563	low bioavailability	545:563	low bioavailability	545:563	The antidepressant venlafaxine hydrochloride (Vx), a highly soluble drug undergoing first pass effect, low bioavailability and short half-life was selected as a challenging payload.
25869450	14	67	theme	urgent	1543:1548	arg1	need					1550:1553	the urgent need	1539:1553	the urgent need for standardized evaluation procedures for mini-tablets	1539:1609	Results point out the urgent need for standardized evaluation procedures for mini-tablets.
25869450	11	68	theme	faster	1236:1241	arg1	release					1243:1249	faster release	1236:1249	faster release	1236:1249	Ethyl cellulose in the core ensured faster release due to polymer migration to the surface and pore formation in the coat.
26652774	0	0	theme	Hydroxyapatite/Native	87:107	arg1	Nanocomposites					129:142	Hydroxyapatite/Native Bacterial Cellulose Nanocomposites	87:142	Hydroxyapatite/Native Bacterial Cellulose Nanocomposites	87:142	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	1	1	from	simulation	201:210	arg1	solutions					266:274	solutions	266:274	solutions of mineral ions	266:290	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
26652774	2	2	theme	ionic	366:370	arg1	composition					372:382	the ionic composition	362:382	the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits	362:493	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	0	3	from	Nanofibrils	10:20	arg1	Synthesis					74:82	Biomimetic Synthesis	63:82	Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites	63:142	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	6	4	theme	water	1056:1060	arg1	models					1062:1067	the two water models	1048:1067	the two water models	1048:1067	The cluster sizes are slightly different for the two water models.
26652774	0	5	theme	Cellulose	119:127	arg1	Nanocomposites					129:142	Hydroxyapatite/Native Bacterial Cellulose Nanocomposites	87:142	Hydroxyapatite/Native Bacterial Cellulose Nanocomposites	87:142	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	3	6	from	influence	514:522	arg1	characteristics					592:606	structural characteristics	581:606	structural characteristics of the simulated nanofibril	581:634	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	3	6	from	influence	514:522	arg1	process					666:672	the crystal nucleation process	643:672	the crystal nucleation process	643:672	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	3	7	theme	TIP3P	545:549	arg1	models					537:542	solvation models	527:542	solvation models (TIP3P and TIP4P-ew water models)	527:576	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	3	7	theme	TIP3P	545:549	arg1	models					570:575	TIP3P and TIP4P-ew water models	545:575	TIP3P and TIP4P-ew water models	545:575	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	5	8	theme	ionic	926:930	arg1	clusters					932:939	ionic clusters	926:939	ionic clusters	926:939	It is shown that ionic clusters are formed in the solution rather than on the fibril surface.
26652774	0	9	theme	Bacterial	109:117	arg1	Nanocomposites					129:142	Hydroxyapatite/Native Bacterial Cellulose Nanocomposites	87:142	Hydroxyapatite/Native Bacterial Cellulose Nanocomposites	87:142	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	9	10	theme	nanofibril	1437:1446	arg1	surfaces					1448:1455	BC nanofibril surfaces	1434:1455	BC nanofibril surfaces	1434:1455	Therefore, the nucleation of CP crystals takes place initially in solution, and then the crystallites formed can be adsorbed on BC nanofibril surfaces.
26652774	4	11	theme	surface	766:772	arg1	layer					774:778	the surface layer	762:778	the surface layer	762:778	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	9	12	theme	CP	1335:1336	arg1	crystals					1338:1345	CP crystals	1335:1345	CP crystals	1335:1345	Therefore, the nucleation of CP crystals takes place initially in solution, and then the crystallites formed can be adsorbed on BC nanofibril surfaces.
26652774	1	13	theme	mineral	279:285	arg1	ions					287:290	mineral ions	279:290	mineral ions	279:290	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
26652774	4	14	dep	particular	747:756	arg1	in					744:745	in	744:745	in	744:745	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	6	15	theme	cluster	1007:1013	arg1	sizes					1015:1019	The cluster sizes	1003:1019	The cluster sizes	1003:1019	The cluster sizes are slightly different for the two water models.
26652774	6	15	theme	cluster	1007:1013	arg1	different					1034:1042	different	1034:1042	different	1034:1042	The cluster sizes are slightly different for the two water models.
26652774	3	16	theme	models	537:542	arg1	influence					514:522	The influence	510:522	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process	510:672	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	2	17	theme	solutions	412:420	arg1	composition					372:382	the ionic composition	362:382	the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits	362:493	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	0	18	theme	Nanocomposites	129:142	arg1	Synthesis					74:82	Biomimetic Synthesis	63:82	Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites	63:142	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	4	19	theme	models	833:838	arg1	characteristics					702:716	The structural characteristics	687:716	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer)	687:779	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	4	19	theme	models	833:838	arg1	independent					804:814	independent	804:814	independent	804:814	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	8	20	theme	BC	1215:1216	arg1	surface					1225:1231	the BC fibril surface	1211:1231	the BC fibril surface	1211:1231	The main conclusion is that the activity of hydroxyl groups on the BC fibril surface is not high enough to cause adsorption of Ca(2+) ions from the solution.
26652774	2	21	theme	CaCl2	406:410	arg1	solutions					412:420	hydroxyapatite and CaCl2 solutions	387:420	hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits	387:493	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	3	22	theme	solvation	527:535	arg1	models					537:542	solvation models	527:542	solvation models (TIP3P and TIP4P-ew water models)	527:576	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	3	22	theme	solvation	527:535	arg1	models					570:575	TIP3P and TIP4P-ew water models	545:575	TIP3P and TIP4P-ew water models	545:575	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	2	23	with	solutions	412:420	arg1	concentrations					431:444	the concentrations	427:444	the concentrations below, equal to, and above the solubility limits	427:493	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	8	24	from	solution	1296:1303	arg1	adsorption					1261:1270	adsorption	1261:1270	adsorption of Ca(2+) ions from the solution	1261:1303	The main conclusion is that the activity of hydroxyl groups on the BC fibril surface is not high enough to cause adsorption of Ca(2+) ions from the solution.
26652774	8	25	theme	groups	1201:1206	arg1	activity					1180:1187	the activity	1176:1187	the activity of hydroxyl groups on the BC fibril surface	1176:1231	The main conclusion is that the activity of hydroxyl groups on the BC fibril surface is not high enough to cause adsorption of Ca(2+) ions from the solution.
26652774	8	25	theme	groups	1201:1206	arg1	high					1240:1243	high	1240:1243	high	1240:1243	The main conclusion is that the activity of hydroxyl groups on the BC fibril surface is not high enough to cause adsorption of Ca(2+) ions from the solution.
26652774	0	26	theme	Cellulose	0:8	arg1	Nanofibrils					10:20	Cellulose Nanofibrils	0:20	Cellulose Nanofibrils	0:20	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	1	27	theme	ions	287:290	arg1	solutions					266:274	solutions	266:274	solutions of mineral ions	266:290	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
26652774	2	28	theme	calcium-phosphate	325:341	arg1	solution					348:355	The supersaturated calcium-phosphate (CP) solution	306:355	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits	306:493	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	2	28	theme	calcium-phosphate	325:341	arg1	simulated					499:507	simulated	499:507	simulated	499:507	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	2	29	theme	hydroxyapatite	387:400	arg1	solutions					412:420	hydroxyapatite and CaCl2 solutions	387:420	hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits	387:493	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	2	30	theme	equal	453:457	arg1	limits					488:493	, equal to, and above the solubility limits	451:493	limits	488:493	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	0	31	theme	Dynamics	155:162	arg1	Simulations					164:174	Molecular Dynamics Simulations	145:174	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.	0:175	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	9	32	theme	crystals	1338:1345	arg1	nucleation					1321:1330	the nucleation	1317:1330	the nucleation of CP crystals	1317:1345	Therefore, the nucleation of CP crystals takes place initially in solution, and then the crystallites formed can be adsorbed on BC nanofibril surfaces.
26652774	2	33	theme	supersaturated	310:323	arg1	solution					348:355	The supersaturated calcium-phosphate (CP) solution	306:355	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits	306:493	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	2	33	theme	supersaturated	310:323	arg1	simulated					499:507	simulated	499:507	simulated	499:507	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	1	34	theme	nanofibril	217:226	arg1	simulation					201:210	Molecular dynamics (MD) simulation	177:210	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions	177:290	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
26652774	8	35	theme	ions	1282:1285	arg1	adsorption					1261:1270	adsorption	1261:1270	adsorption of Ca(2+) ions from the solution	1261:1303	The main conclusion is that the activity of hydroxyl groups on the BC fibril surface is not high enough to cause adsorption of Ca(2+) ions from the solution.
26652774	0	36	theme	Molecular	145:153	arg1	Simulations					164:174	Molecular Dynamics Simulations	145:174	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.	0:175	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	3	37	theme	crystal	647:653	arg1	process					666:672	the crystal nucleation process	643:672	the crystal nucleation process	643:672	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	3	38	theme	water	564:568	arg1	models					537:542	solvation models	527:542	solvation models (TIP3P and TIP4P-ew water models)	527:576	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	3	38	theme	water	564:568	arg1	models					570:575	TIP3P and TIP4P-ew water models	545:575	TIP3P and TIP4P-ew water models	545:575	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	5	39	theme	fibril	987:992	arg1	surface					994:1000	the fibril surface	983:1000	the fibril surface	983:1000	It is shown that ionic clusters are formed in the solution rather than on the fibril surface.
26652774	4	40	theme	structural	691:700	arg1	characteristics					702:716	The structural characteristics	687:716	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer)	687:779	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	4	40	theme	structural	691:700	arg1	independent					804:814	independent	804:814	independent	804:814	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	7	41	theme	interaction	1096:1106	arg1	parameters					1108:1117	the ion-ion interaction parameters	1084:1117	the ion-ion interaction parameters	1084:1117	The effect of the ion-ion interaction parameters on the results is discussed.
26652774	4	42	dep	nanofibrils	731:741	arg1	particular					747:756	particular	747:756	particular	747:756	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	3	43	theme	nucleation	655:664	arg1	process					666:672	the crystal nucleation process	643:672	the crystal nucleation process	643:672	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	8	44	theme	hydroxyl	1192:1199	arg1	groups					1201:1206	hydroxyl groups	1192:1206	hydroxyl groups	1192:1206	The main conclusion is that the activity of hydroxyl groups on the BC fibril surface is not high enough to cause adsorption of Ca(2+) ions from the solution.
26652774	1	45	theme	native	231:236	arg1	BC					259:260	BC	259:260	BC	259:260	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
26652774	1	45	theme	native	231:236	arg1	cellulose					248:256	native bacterial cellulose	231:256	native bacterial cellulose (BC)	231:261	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
26652774	7	46	theme	parameters	1108:1117	arg1	effect					1074:1079	The effect	1070:1079	The effect of the ion-ion interaction parameters on the results	1070:1132	The effect of the ion-ion interaction parameters on the results is discussed.
26652774	4	47	from	solutions	898:906	arg1	presence					874:881	the presence	870:881	the presence of ions in the solutions	870:906	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	8	48	theme	main	1152:1155	arg1	conclusion					1157:1166	The main conclusion	1148:1166	The main conclusion	1148:1166	The main conclusion is that the activity of hydroxyl groups on the BC fibril surface is not high enough to cause adsorption of Ca(2+) ions from the solution.
26652774	1	49	theme	bacterial	238:246	arg1	BC					259:260	BC	259:260	BC	259:260	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
26652774	1	49	theme	bacterial	238:246	arg1	cellulose					248:256	native bacterial cellulose	231:256	native bacterial cellulose (BC)	231:261	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
26652774	8	50	theme	Ca	1275:1276	arg1	ions					1282:1285	Ca(2+) ions	1275:1285	Ca(2+) ions	1275:1285	The main conclusion is that the activity of hydroxyl groups on the BC fibril surface is not high enough to cause adsorption of Ca(2+) ions from the solution.
26652774	2	51	with	solution	348:355	arg1	composition					372:382	the ionic composition	362:382	the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits	362:493	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	4	52	theme	solvation	823:831	arg1	models					833:838	the solvation models	819:838	the solvation models used in the simulation	819:861	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	4	53	theme	nanofibrils	731:741	arg1	characteristics					702:716	The structural characteristics	687:716	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer)	687:779	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	4	53	theme	nanofibrils	731:741	arg1	independent					804:814	independent	804:814	independent	804:814	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	0	54	theme	Mineralization	45:58	arg1	Mechanism					26:34	Mechanism	26:34	Mechanism	26:34	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	0	54	theme	Mineralization	45:58	arg1	Nanofibrils					10:20	Cellulose Nanofibrils	0:20	Cellulose Nanofibrils	0:20	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	7	55	from	effect	1074:1079	arg1	results					1126:1132	the results	1122:1132	the results	1122:1132	The effect of the ion-ion interaction parameters on the results is discussed.
26652774	9	56	theme	BC	1434:1435	arg1	surfaces					1448:1455	BC nanofibril surfaces	1434:1455	BC nanofibril surfaces	1434:1455	Therefore, the nucleation of CP crystals takes place initially in solution, and then the crystallites formed can be adsorbed on BC nanofibril surfaces.
26652774	3	57	theme	structural	581:590	arg1	characteristics					592:606	structural characteristics	581:606	structural characteristics of the simulated nanofibril	581:634	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	4	58	theme	cellulose	721:729	arg1	nanofibrils					731:741	cellulose nanofibrils	721:741	cellulose nanofibrils	721:741	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	7	59	theme	ion-ion	1088:1094	arg1	parameters					1108:1117	the ion-ion interaction parameters	1084:1117	the ion-ion interaction parameters	1084:1117	The effect of the ion-ion interaction parameters on the results is discussed.
26652774	4	60	from	ions	886:889	arg1	solutions					898:906	the solutions	894:906	the solutions	894:906	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	3	61	theme	nanofibril	625:634	arg1	characteristics					592:606	structural characteristics	581:606	structural characteristics of the simulated nanofibril	581:634	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	8	62	from	activity	1180:1187	arg1	surface					1225:1231	the BC fibril surface	1211:1231	the BC fibril surface	1211:1231	The main conclusion is that the activity of hydroxyl groups on the BC fibril surface is not high enough to cause adsorption of Ca(2+) ions from the solution.
26652774	3	63	theme	TIP4P-ew	555:562	arg1	models					537:542	solvation models	527:542	solvation models (TIP3P and TIP4P-ew water models)	527:576	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	3	63	theme	TIP4P-ew	555:562	arg1	models					570:575	TIP3P and TIP4P-ew water models	545:575	TIP3P and TIP4P-ew water models	545:575	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	1	64	theme	cellulose	248:256	arg1	nanofibril					217:226	a nanofibril	215:226	a nanofibril of native bacterial cellulose (BC)	215:261	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
26652774	4	65	theme	ions	886:889	arg1	presence					874:881	the presence	870:881	the presence of ions in the solutions	870:906	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	8	66	theme	fibril	1218:1223	arg1	surface					1225:1231	the BC fibril surface	1211:1231	the BC fibril surface	1211:1231	The main conclusion is that the activity of hydroxyl groups on the BC fibril surface is not high enough to cause adsorption of Ca(2+) ions from the solution.
26652774	4	67	theme	layer	774:778	arg1	particular					747:756	particular	747:756	particular	747:756	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	2	68	theme	CP	344:345	arg1	solution					348:355	The supersaturated calcium-phosphate (CP) solution	306:355	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits	306:493	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	2	68	theme	CP	344:345	arg1	simulated					499:507	simulated	499:507	simulated	499:507	The supersaturated calcium-phosphate (CP) solution with the ionic composition of hydroxyapatite and CaCl2 solutions with the concentrations below, equal to, and above the solubility limits are simulated.
26652774	4	69	from	presence	874:881	arg1	solutions					898:906	the solutions	894:906	the solutions	894:906	The structural characteristics of cellulose nanofibrils (in particular, of the surface layer) are found to be nearly independent of the solvation models used in the simulation and on the presence of ions in the solutions.
26652774	0	70	dep	Nanofibrils	10:20	arg1	Simulations					164:174	Molecular Dynamics Simulations	145:174	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.	0:175	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	1	71	theme	Molecular	177:185	arg1	dynamics					187:194	Molecular dynamics	177:194	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions	177:290	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
26652774	1	71	theme	Molecular	177:185	arg1	MD					197:198	MD	197:198	MD	197:198	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
26652774	0	72	theme	Biomimetic	63:72	arg1	Synthesis					74:82	Biomimetic Synthesis	63:82	Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites	63:142	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	0	73	from	Mechanism	26:34	arg1	Synthesis					74:82	Biomimetic Synthesis	63:82	Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites	63:142	Cellulose Nanofibrils and Mechanism of their Mineralization in Biomimetic Synthesis of Hydroxyapatite/Native Bacterial Cellulose Nanocomposites: Molecular Dynamics Simulations.
26652774	3	74	theme	simulated	615:623	arg1	nanofibril					625:634	the simulated nanofibril	611:634	the simulated nanofibril	611:634	The influence of solvation models (TIP3P and TIP4P-ew water models) on structural characteristics of the simulated nanofibril and on the crystal nucleation process is assessed.
26652774	1	75	theme	dynamics	187:194	arg1	simulation					201:210	Molecular dynamics (MD) simulation	177:210	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions	177:290	Molecular dynamics (MD) simulation of a nanofibril of native bacterial cellulose (BC) in solutions of mineral ions is presented.
24420263	2	0	from	variations	359:368	arg1	elements					414:421	major elements	408:421	major elements	408:421	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	2	0	from	variations	359:368	arg1	THg					398:400	THg	398:400	THg	398:400	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	2	0	from	variations	359:368	arg1	MeHg					382:385	sediment MeHg	373:385	sediment MeHg	373:385	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	2	0	from	variations	359:368	arg1	Hg					394:395	total Hg	388:395	total Hg (THg)	388:401	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	4	1	theme	subsequent	702:711	arg1	erosion					713:719	subsequent erosion	702:719	subsequent erosion	702:719	Notably, fiber decomposition and subsequent erosion of fiber residues will cause buried contaminants to gradually come closer to the sediment-water interface.
24420263	1	2	theme	worldwide	269:277	arg1	threat					279:284	a worldwide threat	267:284	a worldwide threat to aquatic ecosystems and human health	267:323	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health.
24420263	6	3	theme	Hg	1053:1054	arg1	methylation					1056:1066	Hg methylation	1053:1066	Hg methylation	1053:1066	As evidenced by correlation patterns and vertical chemical profiles, reduced S may have fueled C-fixation and Hg methylation at this site.
24420263	1	4	theme	organic	202:208	arg1	OM					218:219	OM	218:219	OM	218:219	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health.
24420263	1	4	theme	organic	202:208	arg1	matter					210:215	organic matter	202:215	organic matter (OM) decomposition	202:234	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health.
24420263	0	5	theme	element	89:95	arg1	composition					97:107	major element composition	83:107	major element composition	83:107	Linking cellulose fiber sediment methyl mercury levels to organic matter decay and major element composition.
24420263	1	6	theme	matter	210:215	arg1	decomposition					222:234	organic matter (OM) decomposition	202:234	organic matter (OM) decomposition	202:234	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health.
24420263	0	7	theme	major	83:87	arg1	composition					97:107	major element composition	83:107	major element composition	83:107	Linking cellulose fiber sediment methyl mercury levels to organic matter decay and major element composition.
24420263	3	8	theme	carbon	574:579	arg1	loss					566:569	loss	566:569	loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S)	566:639	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	4	9	theme	fiber	678:682	arg1	decomposition					684:696	fiber decomposition	678:696	fiber decomposition	678:696	Notably, fiber decomposition and subsequent erosion of fiber residues will cause buried contaminants to gradually come closer to the sediment-water interface.
24420263	6	10	theme	correlation	959:969	arg1	patterns					971:978	correlation patterns	959:978	correlation patterns	959:978	As evidenced by correlation patterns and vertical chemical profiles, reduced S may have fueled C-fixation and Hg methylation at this site.
24420263	4	11	theme	sediment-water	802:815	arg1	interface					817:825	the sediment-water interface	798:825	the sediment-water interface	798:825	Notably, fiber decomposition and subsequent erosion of fiber residues will cause buried contaminants to gradually come closer to the sediment-water interface.
24420263	1	12	theme	aquatic	289:295	arg1	ecosystems					297:306	aquatic ecosystems	289:306	aquatic ecosystems	289:306	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health.
24420263	1	13	theme	toxic	148:152	arg1	Hg					161:162	highly toxic methyl Hg	141:162	highly toxic methyl Hg (MeHg)	141:169	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health.
24420263	1	13	theme	toxic	148:152	arg1	MeHg					165:168	MeHg	165:168	MeHg	165:168	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health.
24420263	2	14	theme	major	408:412	arg1	elements					414:421	major elements	408:421	major elements	408:421	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	1	15	theme	methyl	154:159	arg1	Hg					161:162	highly toxic methyl Hg	141:162	highly toxic methyl Hg (MeHg)	141:169	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health.
24420263	1	15	theme	methyl	154:159	arg1	MeHg					165:168	MeHg	165:168	MeHg	165:168	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health.
24420263	0	16	theme	cellulose	8:16	arg1	levels					48:53	cellulose fiber sediment methyl mercury levels	8:53	cellulose fiber sediment methyl mercury levels	8:53	Linking cellulose fiber sediment methyl mercury levels to organic matter decay and major element composition.
24420263	3	17	theme	uptake	586:591	arg1	loss					566:569	loss	566:569	loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S)	566:639	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	3	18	theme	nitrogen	596:603	arg1	sulfur					630:635	sulfur	630:635	sulfur (S)	630:639	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	3	18	theme	nitrogen	596:603	arg1	uptake					586:591	uptake	586:591	uptake of nitrogen (N)	586:607	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	3	18	theme	nitrogen	596:603	arg1	phosphorus					610:619	phosphorus	610:619	phosphorus (P)	610:623	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	3	18	theme	nitrogen	596:603	arg1	carbon					574:579	carbon	574:579	carbon (C)	574:583	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	3	18	theme	nitrogen	596:603	arg1	P					622:622	P	622:622	P	622:622	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	3	18	theme	nitrogen	596:603	arg1	S					638:638	S	638:638	S	638:638	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	3	18	theme	nitrogen	596:603	arg1	C					582:582	C	582:582	C	582:582	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	2	19	theme	sediment	373:380	arg1	MeHg					382:385	sediment MeHg	373:385	sediment MeHg	373:385	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	0	20	theme	sediment	24:31	arg1	levels					48:53	cellulose fiber sediment methyl mercury levels	8:53	cellulose fiber sediment methyl mercury levels	8:53	Linking cellulose fiber sediment methyl mercury levels to organic matter decay and major element composition.
24420263	4	21	theme	residues	730:737	arg1	erosion					713:719	subsequent erosion	702:719	subsequent erosion	702:719	Notably, fiber decomposition and subsequent erosion of fiber residues will cause buried contaminants to gradually come closer to the sediment-water interface.
24420263	4	21	theme	residues	730:737	arg1	decomposition					684:696	fiber decomposition	678:696	fiber decomposition	678:696	Notably, fiber decomposition and subsequent erosion of fiber residues will cause buried contaminants to gradually come closer to the sediment-water interface.
24420263	2	22	theme	spatial	351:357	arg1	variations					359:368	temporal and spatial variations	338:368	temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements	338:421	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	0	23	theme	fiber	18:22	arg1	levels					48:53	cellulose fiber sediment methyl mercury levels	8:53	cellulose fiber sediment methyl mercury levels	8:53	Linking cellulose fiber sediment methyl mercury levels to organic matter decay and major element composition.
24420263	4	24	theme	fiber	724:728	arg1	residues					730:737	fiber residues	724:737	fiber residues	724:737	Notably, fiber decomposition and subsequent erosion of fiber residues will cause buried contaminants to gradually come closer to the sediment-water interface.
24420263	1	25	theme	human	312:316	arg1	health					318:323	human health	312:323	human health	312:323	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health.
24420263	0	26	theme	mercury	40:46	arg1	levels					48:53	cellulose fiber sediment methyl mercury levels	8:53	cellulose fiber sediment methyl mercury levels	8:53	Linking cellulose fiber sediment methyl mercury levels to organic matter decay and major element composition.
24420263	5	27	from	loss	912:915	arg1	C					904:904	C	904:904	C	904:904	At an adjacent site where decomposed fiber accumulated, there was a gain in C and a loss of S when MeHg increased.
24420263	3	28	theme	Fiber	497:501	arg1	decomposition					503:515	Fiber decomposition	497:515	Fiber decomposition	497:515	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	2	29	theme	temporal	338:345	arg1	variations					359:368	temporal and spatial variations	338:368	temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements	338:421	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	0	30	theme	methyl	33:38	arg1	levels					48:53	cellulose fiber sediment methyl mercury levels	8:53	cellulose fiber sediment methyl mercury levels	8:53	Linking cellulose fiber sediment methyl mercury levels to organic matter decay and major element composition.
24420263	2	31	theme	freshwater	428:437	arg1	lagoon					439:444	a freshwater lagoon	426:444	a freshwater lagoon in Sweden	426:454	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	3	32	theme	narrow	532:537	arg1	layer					547:551	a narrow surface layer	530:551	a narrow surface layer	530:551	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	5	33	theme	adjacent	834:841	arg1	site					843:846	an adjacent site	831:846	an adjacent site where decomposed fiber accumulated	831:881	At an adjacent site where decomposed fiber accumulated, there was a gain in C and a loss of S when MeHg increased.
24420263	2	34	theme	total	388:392	arg1	THg					398:400	THg	398:400	THg	398:400	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	2	34	theme	total	388:392	arg1	Hg					394:395	total Hg	388:395	total Hg (THg)	388:401	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	2	35	theme	cellulose	479:487	arg1	fibers					489:494	Hg-laden cellulose fibers	470:494	Hg-laden cellulose fibers	470:494	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	3	36	theme	surface	539:545	arg1	layer					547:551	a narrow surface layer	530:551	a narrow surface layer	530:551	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	6	37	theme	reduced	1012:1018	arg1	S					1020:1020	reduced S	1012:1020	reduced S	1012:1020	As evidenced by correlation patterns and vertical chemical profiles, reduced S may have fueled C-fixation and Hg methylation at this site.
24420263	5	38	theme	S	920:920	arg1	loss					912:915	a loss	910:915	a loss of S	910:920	At an adjacent site where decomposed fiber accumulated, there was a gain in C and a loss of S when MeHg increased.
24420263	5	38	theme	S	920:920	arg1	gain					896:899	a gain	894:899	a gain in C	894:904	At an adjacent site where decomposed fiber accumulated, there was a gain in C and a loss of S when MeHg increased.
24420263	3	39	theme	phosphorus	610:619	arg1	loss					566:569	loss	566:569	loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S)	566:639	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	2	40	theme	Hg-laden	470:477	arg1	fibers					489:494	Hg-laden cellulose fibers	470:494	Hg-laden cellulose fibers	470:494	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	5	41	theme	decomposed	854:863	arg1	fiber					865:869	decomposed fiber	854:869	decomposed fiber	854:869	At an adjacent site where decomposed fiber accumulated, there was a gain in C and a loss of S when MeHg increased.
24420263	1	42	theme	mercury	125:131	arg1	Methylation					110:120	Methylation	110:120	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia,	110:251	Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health.
24420263	2	43	from	lagoon	439:444	arg1	Sweden					449:454	Sweden	449:454	Sweden	449:454	We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and major elements in a freshwater lagoon in Sweden polluted with Hg-laden cellulose fibers.
24420263	6	44	theme	chemical	993:1000	arg1	profiles					1002:1009	vertical chemical profiles	984:1009	vertical chemical profiles	984:1009	As evidenced by correlation patterns and vertical chemical profiles, reduced S may have fueled C-fixation and Hg methylation at this site.
24420263	5	45	from	gain	896:899	arg1	C					904:904	C	904:904	C	904:904	At an adjacent site where decomposed fiber accumulated, there was a gain in C and a loss of S when MeHg increased.
24420263	0	46	theme	matter	66:71	arg1	decay					73:77	organic matter decay	58:77	organic matter decay	58:77	Linking cellulose fiber sediment methyl mercury levels to organic matter decay and major element composition.
24420263	6	47	theme	vertical	984:991	arg1	profiles					1002:1009	vertical chemical profiles	984:1009	vertical chemical profiles	984:1009	As evidenced by correlation patterns and vertical chemical profiles, reduced S may have fueled C-fixation and Hg methylation at this site.
24420263	3	48	theme	MeHg	656:659	arg1	levels					661:666	MeHg levels	656:666	MeHg levels	656:666	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	0	49	theme	organic	58:64	arg1	decay					73:77	organic matter decay	58:77	organic matter decay	58:77	Linking cellulose fiber sediment methyl mercury levels to organic matter decay and major element composition.
24420263	3	50	theme	sulfur	630:635	arg1	loss					566:569	loss	566:569	loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S)	566:639	Fiber decomposition, confined to a narrow surface layer, resulted in loss of carbon (C), uptake of nitrogen (N), phosphorus (P), and sulfur (S), and increased MeHg levels.
24420263	4	51	theme	buried	750:755	arg1	contaminants					757:768	buried contaminants	750:768	buried contaminants	750:768	Notably, fiber decomposition and subsequent erosion of fiber residues will cause buried contaminants to gradually come closer to the sediment-water interface.
24274511	0	0	theme	thermal	67:73	arg1	study					49:53	study	49:53	study of tensile, thermal and oxygen barrier properties	49:103	Dispersion of SiC nanoparticles in cellulose for study of tensile, thermal and oxygen barrier properties.
24274511	9	1	theme	silicon	982:988	arg1	concentrations					998:1011	silicon carbide concentrations	982:1011	silicon carbide concentrations	982:1011	A substantial reduction in oxygen permeability was obtained with increase in silicon carbide concentrations.
24274511	5	2	theme	Thermal	558:564	arg1	stabilities					566:576	Thermal stabilities	558:576	Thermal stabilities of cellulose/SiC nanobiocomposites	558:611	Thermal stabilities of cellulose/SiC nanobiocomposites were studied by thermogravimetric analysis (TGA).
24274511	6	3	theme	cellulose/SiC	667:679	arg1	nanobiocomposites					681:697	The cellulose/SiC nanobiocomposites	663:697	The cellulose/SiC nanobiocomposites	663:697	The cellulose/SiC nanobiocomposites were thermally more stable than the raw cellulose.
24274511	6	3	theme	cellulose/SiC	667:679	arg1	stable					719:724	stable	719:724	stable	719:724	The cellulose/SiC nanobiocomposites were thermally more stable than the raw cellulose.
24274511	9	4	theme	carbide	990:996	arg1	concentrations					998:1011	silicon carbide concentrations	982:1011	silicon carbide concentrations	982:1011	A substantial reduction in oxygen permeability was obtained with increase in silicon carbide concentrations.
24274511	2	5	with	interaction	207:217	arg1	cellulose					245:253	cellulose	245:253	cellulose	245:253	The interaction of SiC nanoparticles with cellulose were confirmed by Fourier transformed infrared (FTIR) spectroscopy.
24274511	8	6	theme	barrier	825:831	arg1	properties					833:842	The oxygen barrier properties	814:842	The oxygen barrier properties of cellulose composites	814:866	The oxygen barrier properties of cellulose composites were measured using gas permeameter.
24274511	0	7	theme	oxygen	79:84	arg1	properties					94:103	oxygen barrier properties	79:103	oxygen barrier properties	79:103	Dispersion of SiC nanoparticles in cellulose for study of tensile, thermal and oxygen barrier properties.
24274511	2	8	theme	FTIR	303:306	arg1	spectroscopy					309:320	Fourier transformed infrared (FTIR) spectroscopy	273:320	Fourier transformed infrared (FTIR) spectroscopy	273:320	The interaction of SiC nanoparticles with cellulose were confirmed by Fourier transformed infrared (FTIR) spectroscopy.
24274511	8	9	theme	cellulose	847:855	arg1	composites					857:866	cellulose composites	847:866	cellulose composites	847:866	The oxygen barrier properties of cellulose composites were measured using gas permeameter.
24274511	3	10	theme	X-ray	392:396	arg1	XRD					411:413	XRD	411:413	XRD	411:413	The structure of cellulose/SiC nanobiocomposites was investigated by X-ray diffraction (XRD), and transmission electron microscopy (TEM).
24274511	3	10	theme	X-ray	392:396	arg1	diffraction					398:408	X-ray diffraction	392:408	X-ray diffraction (XRD)	392:414	The structure of cellulose/SiC nanobiocomposites was investigated by X-ray diffraction (XRD), and transmission electron microscopy (TEM).
24274511	9	11	from	reduction	919:927	arg1	permeability					939:950	oxygen permeability	932:950	oxygen permeability	932:950	A substantial reduction in oxygen permeability was obtained with increase in silicon carbide concentrations.
24274511	0	12	theme	properties	94:103	arg1	study					49:53	study	49:53	study of tensile, thermal and oxygen barrier properties	49:103	Dispersion of SiC nanoparticles in cellulose for study of tensile, thermal and oxygen barrier properties.
24274511	8	13	theme	gas	888:890	arg1	permeameter					892:902	gas permeameter	888:902	gas permeameter	888:902	The oxygen barrier properties of cellulose composites were measured using gas permeameter.
24274511	2	14	theme	infrared	293:300	arg1	spectroscopy					309:320	Fourier transformed infrared (FTIR) spectroscopy	273:320	Fourier transformed infrared (FTIR) spectroscopy	273:320	The interaction of SiC nanoparticles with cellulose were confirmed by Fourier transformed infrared (FTIR) spectroscopy.
24274511	0	15	theme	barrier	86:92	arg1	properties					94:103	oxygen barrier properties	79:103	oxygen barrier properties	79:103	Dispersion of SiC nanoparticles in cellulose for study of tensile, thermal and oxygen barrier properties.
24274511	2	16	theme	transformed	281:291	arg1	spectroscopy					309:320	Fourier transformed infrared (FTIR) spectroscopy	273:320	Fourier transformed infrared (FTIR) spectroscopy	273:320	The interaction of SiC nanoparticles with cellulose were confirmed by Fourier transformed infrared (FTIR) spectroscopy.
24274511	4	17	theme	nanobiocomposites	491:507	arg1	properties					473:482	The tensile properties	461:482	The tensile properties of the nanobiocomposites	461:507	The tensile properties of the nanobiocomposites were improved as compared with virgin cellulose.
24274511	0	18	theme	tensile	58:64	arg1	study					49:53	study	49:53	study of tensile, thermal and oxygen barrier properties	49:103	Dispersion of SiC nanoparticles in cellulose for study of tensile, thermal and oxygen barrier properties.
24274511	1	19	theme	solution	183:190	arg1	technique					192:200	solution technique	183:200	solution technique	183:200	Cellulose/silicon carbide (cellulose/SiC) nanobiocomposites were prepared by solution technique.
24274511	9	20	theme	substantial	907:917	arg1	reduction					919:927	A substantial reduction	905:927	A substantial reduction in oxygen permeability	905:950	A substantial reduction in oxygen permeability was obtained with increase in silicon carbide concentrations.
24274511	5	21	theme	thermogravimetric	629:645	arg1	TGA					657:659	TGA	657:659	TGA	657:659	Thermal stabilities of cellulose/SiC nanobiocomposites were studied by thermogravimetric analysis (TGA).
24274511	5	21	theme	thermogravimetric	629:645	arg1	analysis					647:654	thermogravimetric analysis	629:654	thermogravimetric analysis (TGA)	629:660	Thermal stabilities of cellulose/SiC nanobiocomposites were studied by thermogravimetric analysis (TGA).
24274511	6	22	theme	raw	735:737	arg1	cellulose					739:747	the raw cellulose	731:747	the raw cellulose	731:747	The cellulose/SiC nanobiocomposites were thermally more stable than the raw cellulose.
24274511	0	23	theme	SiC	14:16	arg1	Dispersion					0:9	Dispersion	0:9	Dispersion of SiC	0:16	Dispersion of SiC nanoparticles in cellulose for study of tensile, thermal and oxygen barrier properties.
24274511	10	24	dep	resistant	1028:1036	arg1	The					1014:1016	The	1014:1016	The	1014:1016	The thermally resistant and oxygen barrier properties of the prepared nanobiocomposites may enable the materials for the packaging applications.
24274511	3	25	theme	transmission	421:432	arg1	TEM					455:457	TEM	455:457	TEM	455:457	The structure of cellulose/SiC nanobiocomposites was investigated by X-ray diffraction (XRD), and transmission electron microscopy (TEM).
24274511	3	25	theme	transmission	421:432	arg1	microscopy					443:452	transmission electron microscopy	421:452	transmission electron microscopy (TEM)	421:458	The structure of cellulose/SiC nanobiocomposites was investigated by X-ray diffraction (XRD), and transmission electron microscopy (TEM).
24274511	10	26	theme	barrier	1049:1055	arg1	properties					1057:1066	oxygen barrier properties	1042:1066	oxygen barrier properties	1042:1066	The thermally resistant and oxygen barrier properties of the prepared nanobiocomposites may enable the materials for the packaging applications.
24274511	3	27	theme	electron	434:441	arg1	TEM					455:457	TEM	455:457	TEM	455:457	The structure of cellulose/SiC nanobiocomposites was investigated by X-ray diffraction (XRD), and transmission electron microscopy (TEM).
24274511	3	27	theme	electron	434:441	arg1	microscopy					443:452	transmission electron microscopy	421:452	transmission electron microscopy (TEM)	421:458	The structure of cellulose/SiC nanobiocomposites was investigated by X-ray diffraction (XRD), and transmission electron microscopy (TEM).
24274511	9	28	theme	oxygen	932:937	arg1	permeability					939:950	oxygen permeability	932:950	oxygen permeability	932:950	A substantial reduction in oxygen permeability was obtained with increase in silicon carbide concentrations.
24274511	10	29	theme	oxygen	1042:1047	arg1	properties					1057:1066	oxygen barrier properties	1042:1066	oxygen barrier properties	1042:1066	The thermally resistant and oxygen barrier properties of the prepared nanobiocomposites may enable the materials for the packaging applications.
24274511	4	30	theme	virgin	540:545	arg1	cellulose					547:555	virgin cellulose	540:555	virgin cellulose	540:555	The tensile properties of the nanobiocomposites were improved as compared with virgin cellulose.
24274511	4	31	theme	tensile	465:471	arg1	properties					473:482	The tensile properties	461:482	The tensile properties of the nanobiocomposites	461:507	The tensile properties of the nanobiocomposites were improved as compared with virgin cellulose.
24274511	2	32	theme	nanoparticles	226:238	arg1	interaction					207:217	The interaction	203:217	The interaction of SiC nanoparticles with cellulose	203:253	The interaction of SiC nanoparticles with cellulose were confirmed by Fourier transformed infrared (FTIR) spectroscopy.
24274511	10	33	theme	packaging	1135:1143	arg1	applications					1145:1156	the packaging applications	1131:1156	the packaging applications	1131:1156	The thermally resistant and oxygen barrier properties of the prepared nanobiocomposites may enable the materials for the packaging applications.
24274511	2	34	theme	SiC	222:224	arg1	nanoparticles					226:238	SiC nanoparticles	222:238	SiC nanoparticles	222:238	The interaction of SiC nanoparticles with cellulose were confirmed by Fourier transformed infrared (FTIR) spectroscopy.
24274511	5	35	theme	cellulose/SiC	581:593	arg1	nanobiocomposites					595:611	cellulose/SiC nanobiocomposites	581:611	cellulose/SiC nanobiocomposites	581:611	Thermal stabilities of cellulose/SiC nanobiocomposites were studied by thermogravimetric analysis (TGA).
24274511	1	36	theme	Cellulose/silicon	106:122	arg1	carbide					124:130	Cellulose/silicon carbide	106:130	Cellulose/silicon carbide (cellulose/SiC) nanobiocomposites	106:164	Cellulose/silicon carbide (cellulose/SiC) nanobiocomposites were prepared by solution technique.
24274511	1	36	theme	Cellulose/silicon	106:122	arg1	cellulose/SiC					133:145	cellulose/SiC	133:145	cellulose/SiC	133:145	Cellulose/silicon carbide (cellulose/SiC) nanobiocomposites were prepared by solution technique.
24274511	10	37	theme	nanobiocomposites	1084:1100	arg1	resistant					1028:1036	resistant	1028:1036	resistant	1028:1036	The thermally resistant and oxygen barrier properties of the prepared nanobiocomposites may enable the materials for the packaging applications.
24274511	10	38	theme	prepared	1075:1082	arg1	nanobiocomposites					1084:1100	the prepared nanobiocomposites	1071:1100	the prepared nanobiocomposites	1071:1100	The thermally resistant and oxygen barrier properties of the prepared nanobiocomposites may enable the materials for the packaging applications.
24274511	2	39	theme	Fourier	273:279	arg1	spectroscopy					309:320	Fourier transformed infrared (FTIR) spectroscopy	273:320	Fourier transformed infrared (FTIR) spectroscopy	273:320	The interaction of SiC nanoparticles with cellulose were confirmed by Fourier transformed infrared (FTIR) spectroscopy.
24274511	5	40	theme	nanobiocomposites	595:611	arg1	stabilities					566:576	Thermal stabilities	558:576	Thermal stabilities of cellulose/SiC nanobiocomposites	558:611	Thermal stabilities of cellulose/SiC nanobiocomposites were studied by thermogravimetric analysis (TGA).
24274511	1	41	theme	carbide	124:130	arg1	nanobiocomposites					148:164	Cellulose/silicon carbide (cellulose/SiC) nanobiocomposites	106:164	Cellulose/silicon carbide (cellulose/SiC) nanobiocomposites	106:164	Cellulose/silicon carbide (cellulose/SiC) nanobiocomposites were prepared by solution technique.
24274511	8	42	theme	composites	857:866	arg1	properties					833:842	The oxygen barrier properties	814:842	The oxygen barrier properties of cellulose composites	814:866	The oxygen barrier properties of cellulose composites were measured using gas permeameter.
24274511	7	43	with	delamination	771:782	arg1	matrix					806:811	cellulose matrix	796:811	cellulose matrix	796:811	It may be due to the delamination of SiC with cellulose matrix.
24274511	9	44	from	increase	970:977	arg1	concentrations					998:1011	silicon carbide concentrations	982:1011	silicon carbide concentrations	982:1011	A substantial reduction in oxygen permeability was obtained with increase in silicon carbide concentrations.
24274511	7	45	theme	cellulose	796:804	arg1	matrix					806:811	cellulose matrix	796:811	cellulose matrix	796:811	It may be due to the delamination of SiC with cellulose matrix.
24274511	3	46	theme	cellulose/SiC	340:352	arg1	nanobiocomposites					354:370	cellulose/SiC nanobiocomposites	340:370	cellulose/SiC nanobiocomposites	340:370	The structure of cellulose/SiC nanobiocomposites was investigated by X-ray diffraction (XRD), and transmission electron microscopy (TEM).
24274511	8	47	theme	oxygen	818:823	arg1	properties					833:842	The oxygen barrier properties	814:842	The oxygen barrier properties of cellulose composites	814:866	The oxygen barrier properties of cellulose composites were measured using gas permeameter.
24274511	3	48	theme	nanobiocomposites	354:370	arg1	structure					327:335	The structure	323:335	The structure of cellulose/SiC nanobiocomposites	323:370	The structure of cellulose/SiC nanobiocomposites was investigated by X-ray diffraction (XRD), and transmission electron microscopy (TEM).
24274511	7	49	theme	SiC	787:789	arg1	delamination					771:782	the delamination	767:782	the delamination of SiC with cellulose matrix	767:811	It may be due to the delamination of SiC with cellulose matrix.
27060639	8	0	theme	chemisorption	1405:1417	arg1	process					1419:1425	a homogeneous monolayer chemisorption process	1381:1425	a homogeneous monolayer chemisorption process	1381:1425	The adsorption isotherms and kinetics indicate that adsorptions of Cr(VI) and MO by CS-CTA-MCM both follow a homogeneous monolayer chemisorption process.
27060639	9	1	theme	little	1603:1608	arg1	capacity					1621:1628	little adsorption capacity	1603:1628	little adsorption capacity loss	1603:1633	This magnetic adsorbent after saturated adsorption could be rapidly separated from water and easily regenerated using dilute NaOH aqueous solutions then virtually reused with little adsorption capacity loss.
27060639	6	2	theme	weak	1007:1010	arg1	solutions					1019:1027	weak acidic solutions	1007:1027	weak acidic solutions	1007:1027	However, at weak acidic solutions, Cr(VI) adsorption is evidently improved due to more efficient Cr(VI) forms, i.e. dichromate and monovalent chromate, binding to this chitosan-based adsorbent.
27060639	2	3	theme	surface	374:380	arg1	morphology					382:391	surface morphology	374:391	surface morphology	374:391	Various techniques were used to characterize the molecular structure, surface morphology, and magnetic feature of this composite adsorbent.
27060639	8	4	theme	MO	1352:1353	arg1	adsorptions					1326:1336	adsorptions	1326:1336	adsorptions of Cr(VI) and MO by CS-CTA-MCM	1326:1367	The adsorption isotherms and kinetics indicate that adsorptions of Cr(VI) and MO by CS-CTA-MCM both follow a homogeneous monolayer chemisorption process.
27060639	0	5	theme	aqueous	67:73	arg1	mixtures					75:82	their aqueous mixtures	61:82	their aqueous mixtures using a quaternary ammonium salt	61:115	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt modified chitosan magnetic composite adsorbent.
27060639	6	6	theme	acidic	1012:1017	arg1	solutions					1019:1027	weak acidic solutions	1007:1027	weak acidic solutions	1007:1027	However, at weak acidic solutions, Cr(VI) adsorption is evidently improved due to more efficient Cr(VI) forms, i.e. dichromate and monovalent chromate, binding to this chitosan-based adsorbent.
27060639	8	7	theme	monolayer	1395:1403	arg1	process					1419:1425	a homogeneous monolayer chemisorption process	1381:1425	a homogeneous monolayer chemisorption process	1381:1425	The adsorption isotherms and kinetics indicate that adsorptions of Cr(VI) and MO by CS-CTA-MCM both follow a homogeneous monolayer chemisorption process.
27060639	3	8	theme	orange	505:510	arg1	removal					476:482	the removal	472:482	the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems	472:583	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	7	9	theme	suitable	1250:1257	arg1	conditions					1262:1271	suitable pH conditions	1250:1271	suitable pH conditions	1250:1271	Thus chromium could be efficient removal together with MO at suitable pH conditions.
27060639	2	10	theme	Various	304:310	arg1	techniques					312:321	Various techniques	304:321	Various techniques	304:321	Various techniques were used to characterize the molecular structure, surface morphology, and magnetic feature of this composite adsorbent.
27060639	3	11	theme	anionic	521:527	arg1	orange					505:510	methyl orange	498:510	methyl orange (MO)	498:515	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	11	theme	anionic	521:527	arg1	dye					529:531	an anionic dye	518:531	an anionic dye	518:531	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	12	attach	removal	476:482	arg2	Cr					487:488	Cr	487:488	Cr(VI)	487:492	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	12	attach	removal	476:482	arg2	MO					513:514	MO	513:514	MO	513:514	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	12	attach	removal	476:482	arg2	orange					505:510	methyl orange	498:510	methyl orange (MO)	498:515	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	12	attach	removal	476:482	arg2	dye					529:531	an anionic dye	518:531	an anionic dye	518:531	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	12	attach	removal	476:482	arg3	systems					577:583	respective single and binary systems	548:583	respective single and binary systems	548:583	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	13	theme	methyl	498:503	arg1	orange					505:510	methyl orange	498:510	methyl orange (MO)	498:515	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	13	theme	methyl	498:503	arg1	dye					529:531	an anionic dye	518:531	an anionic dye	518:531	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	13	theme	methyl	498:503	arg1	MO					513:514	MO	513:514	MO	513:514	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	7	14	from	conditions	1262:1271	arg1	removal					1222:1228	efficient removal	1212:1228	efficient removal together with MO	1212:1245	Thus chromium could be efficient removal together with MO at suitable pH conditions.
27060639	0	15	theme	ammonium	103:110	arg1	salt					112:115	a quaternary ammonium salt	90:115	a quaternary ammonium salt	90:115	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt modified chitosan magnetic composite adsorbent.
27060639	4	16	theme	improved	686:693	arg1	capacities					706:715	evidently improved adsorption capacities	676:715	evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups	676:794	Compared with chitosan magnetic adsorbent (CS-MCM) without modification, CS-CTA-MCM shows evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups.
27060639	9	17	theme	adsorption	1610:1619	arg1	capacity					1621:1628	little adsorption capacity	1603:1628	little adsorption capacity loss	1603:1633	This magnetic adsorbent after saturated adsorption could be rapidly separated from water and easily regenerated using dilute NaOH aqueous solutions then virtually reused with little adsorption capacity loss.
27060639	2	18	theme	adsorbent	433:441	arg1	feature					407:413	magnetic feature	398:413	magnetic feature	398:413	Various techniques were used to characterize the molecular structure, surface morphology, and magnetic feature of this composite adsorbent.
27060639	2	18	theme	adsorbent	433:441	arg1	structure					363:371	molecular structure	353:371	molecular structure	353:371	Various techniques were used to characterize the molecular structure, surface morphology, and magnetic feature of this composite adsorbent.
27060639	2	18	theme	adsorbent	433:441	arg1	morphology					382:391	surface morphology	374:391	surface morphology	374:391	Various techniques were used to characterize the molecular structure, surface morphology, and magnetic feature of this composite adsorbent.
27060639	4	19	theme	quaternary	764:773	arg1	groups					789:794	the additional quaternary ammonium salt groups	749:794	the additional quaternary ammonium salt groups	749:794	Compared with chitosan magnetic adsorbent (CS-MCM) without modification, CS-CTA-MCM shows evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups.
27060639	2	20	theme	magnetic	398:405	arg1	feature					407:413	magnetic feature	398:413	magnetic feature	398:413	Various techniques were used to characterize the molecular structure, surface morphology, and magnetic feature of this composite adsorbent.
27060639	1	21	theme	quaternary	167:176	arg1	composite					219:227	A quaternary ammonium salt modified chitosan magnetic composite adsorbent	165:237	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM)	165:250	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	1	21	theme	quaternary	167:176	arg1	CS-CTA-MCM					240:249	CS-CTA-MCM	240:249	CS-CTA-MCM	240:249	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	0	22	theme	quaternary	92:101	arg1	ammonium					103:110	a quaternary ammonium	90:110	a quaternary ammonium salt	90:115	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt modified chitosan magnetic composite adsorbent.
27060639	9	23	theme	capacity	1621:1628	arg1	loss					1630:1633	little adsorption capacity loss	1603:1633	little adsorption capacity loss	1603:1633	This magnetic adsorbent after saturated adsorption could be rapidly separated from water and easily regenerated using dilute NaOH aqueous solutions then virtually reused with little adsorption capacity loss.
27060639	9	24	theme	NaOH	1553:1556	arg1	solutions					1566:1574	dilute NaOH aqueous solutions	1546:1574	dilute NaOH aqueous solutions then virtually reused with little adsorption capacity loss	1546:1633	This magnetic adsorbent after saturated adsorption could be rapidly separated from water and easily regenerated using dilute NaOH aqueous solutions then virtually reused with little adsorption capacity loss.
27060639	4	25	theme	additional	753:762	arg1	groups					789:794	the additional quaternary ammonium salt groups	749:794	the additional quaternary ammonium salt groups	749:794	Compared with chitosan magnetic adsorbent (CS-MCM) without modification, CS-CTA-MCM shows evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups.
27060639	1	26	theme	ammonium	178:185	arg1	composite					219:227	A quaternary ammonium salt modified chitosan magnetic composite adsorbent	165:237	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM)	165:250	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	1	26	theme	ammonium	178:185	arg1	CS-CTA-MCM					240:249	CS-CTA-MCM	240:249	CS-CTA-MCM	240:249	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	5	27	theme	considerable	899:910	arg1	extent					912:917	a considerable extent	897:917	a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture	897:992	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	5	28	from	ions	964:967	arg1	mixture					986:992	their aqueous mixture	972:992	their aqueous mixture	972:992	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	1	29	theme	salt	187:190	arg1	composite					219:227	A quaternary ammonium salt modified chitosan magnetic composite adsorbent	165:237	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM)	165:250	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	1	29	theme	salt	187:190	arg1	CS-CTA-MCM					240:249	CS-CTA-MCM	240:249	CS-CTA-MCM	240:249	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	0	30	theme	Efficient	0:8	arg1	adsorption					10:19	Efficient adsorption	0:19	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt	0:115	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt modified chitosan magnetic composite adsorbent.
27060639	1	31	theme	Fe3O4	283:287	arg1	nanoparticles					289:301	Fe3O4 nanoparticles	283:301	Fe3O4 nanoparticles	283:301	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	8	32	theme	homogeneous	1383:1393	arg1	process					1419:1425	a homogeneous monolayer chemisorption process	1381:1425	a homogeneous monolayer chemisorption process	1381:1425	The adsorption isotherms and kinetics indicate that adsorptions of Cr(VI) and MO by CS-CTA-MCM both follow a homogeneous monolayer chemisorption process.
27060639	5	33	theme	more	849:852	arg1	affinity					854:861	more affinity	849:861	more affinity to CS-CTA-MCM than Cr	849:883	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	8	34	theme	adsorption	1278:1287	arg1	isotherms					1289:1297	The adsorption isotherms	1274:1297	The adsorption isotherms	1274:1297	The adsorption isotherms and kinetics indicate that adsorptions of Cr(VI) and MO by CS-CTA-MCM both follow a homogeneous monolayer chemisorption process.
27060639	1	35	theme	modified	192:199	arg1	composite					219:227	A quaternary ammonium salt modified chitosan magnetic composite adsorbent	165:237	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM)	165:250	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	1	35	theme	modified	192:199	arg1	CS-CTA-MCM					240:249	CS-CTA-MCM	240:249	CS-CTA-MCM	240:249	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	1	36	theme	nanoparticles	289:301	arg1	combination					268:278	combination	268:278	combination of Fe3O4 nanoparticles	268:301	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	5	37	contain	bears	843:847	arg1	MO					840:841	MO	840:841	MO	840:841	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	5	37	contain	bears	843:847	arg2	affinity					854:861	more affinity	849:861	more affinity to CS-CTA-MCM than Cr	849:883	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	6	38	theme	efficient	1082:1090	arg1	Cr					1092:1093	more efficient Cr(VI) forms	1077:1103	more efficient Cr(VI) forms	1077:1103	However, at weak acidic solutions, Cr(VI) adsorption is evidently improved due to more efficient Cr(VI) forms, i.e. dichromate and monovalent chromate, binding to this chitosan-based adsorbent.
27060639	6	38	theme	efficient	1082:1090	arg1	dichromate					1111:1120	dichromate	1111:1120	dichromate	1111:1120	However, at weak acidic solutions, Cr(VI) adsorption is evidently improved due to more efficient Cr(VI) forms, i.e. dichromate and monovalent chromate, binding to this chitosan-based adsorbent.
27060639	6	38	theme	efficient	1082:1090	arg1	chromate					1137:1144	monovalent chromate	1126:1144	monovalent chromate	1126:1144	However, at weak acidic solutions, Cr(VI) adsorption is evidently improved due to more efficient Cr(VI) forms, i.e. dichromate and monovalent chromate, binding to this chitosan-based adsorbent.
27060639	5	39	theme	aqueous	978:984	arg1	mixture					986:992	their aqueous mixture	972:992	their aqueous mixture	972:992	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	1	40	theme	chitosan	201:208	arg1	composite					219:227	A quaternary ammonium salt modified chitosan magnetic composite adsorbent	165:237	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM)	165:250	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	1	40	theme	chitosan	201:208	arg1	CS-CTA-MCM					240:249	CS-CTA-MCM	240:249	CS-CTA-MCM	240:249	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	5	41	theme	adsorption	810:819	arg1	study					833:837	the adsorption equilibrium study	806:837	the adsorption equilibrium study	806:837	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	5	42	theme	dye	949:951	arg1	adsorption					935:944	preferential adsorption	922:944	preferential adsorption of dye over metal ions in their aqueous mixture	922:992	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	0	43	from	mixtures	75:82	arg1	adsorption					10:19	Efficient adsorption	0:19	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt	0:115	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt modified chitosan magnetic composite adsorbent.
27060639	5	44	theme	preferential	922:933	arg1	adsorption					935:944	preferential adsorption	922:944	preferential adsorption of dye over metal ions in their aqueous mixture	922:992	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	9	45	theme	saturated	1458:1466	arg1	adsorption					1468:1477	saturated adsorption	1458:1477	saturated adsorption	1458:1477	This magnetic adsorbent after saturated adsorption could be rapidly separated from water and easily regenerated using dilute NaOH aqueous solutions then virtually reused with little adsorption capacity loss.
27060639	1	46	theme	magnetic	210:217	arg1	composite					219:227	A quaternary ammonium salt modified chitosan magnetic composite adsorbent	165:237	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM)	165:250	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	1	46	theme	magnetic	210:217	arg1	CS-CTA-MCM					240:249	CS-CTA-MCM	240:249	CS-CTA-MCM	240:249	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	0	47	theme	orange	36:41	arg1	adsorption					10:19	Efficient adsorption	0:19	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt	0:115	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt modified chitosan magnetic composite adsorbent.
27060639	5	48	theme	adsorption	935:944	arg1	extent					912:917	a considerable extent	897:917	a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture	897:992	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	7	49	theme	efficient	1212:1220	arg1	removal					1222:1228	efficient removal	1212:1228	efficient removal together with MO	1212:1245	Thus chromium could be efficient removal together with MO at suitable pH conditions.
27060639	3	50	theme	single	559:564	arg1	systems					577:583	respective single and binary systems	548:583	respective single and binary systems	548:583	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	6	51	dep	Cr	1092:1093	arg1	forms					1099:1103	forms	1099:1103	more efficient Cr(VI) forms	1077:1103	However, at weak acidic solutions, Cr(VI) adsorption is evidently improved due to more efficient Cr(VI) forms, i.e. dichromate and monovalent chromate, binding to this chitosan-based adsorbent.
27060639	6	51	dep	Cr	1092:1093	arg1	VI					1095:1096	VI	1095:1096	VI	1095:1096	However, at weak acidic solutions, Cr(VI) adsorption is evidently improved due to more efficient Cr(VI) forms, i.e. dichromate and monovalent chromate, binding to this chitosan-based adsorbent.
27060639	4	52	theme	adsorbent	618:626	arg1	CS-MCM					629:634	CS-MCM	629:634	CS-MCM	629:634	Compared with chitosan magnetic adsorbent (CS-MCM) without modification, CS-CTA-MCM shows evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups.
27060639	4	52	theme	adsorbent	618:626	arg1	chitosan					600:607	chitosan magnetic adsorbent	600:626	chitosan magnetic adsorbent (CS-MCM) without modification	600:656	Compared with chitosan magnetic adsorbent (CS-MCM) without modification, CS-CTA-MCM shows evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups.
27060639	0	53	theme	methyl	29:34	arg1	orange					36:41	methyl orange	29:41	methyl orange	29:41	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt modified chitosan magnetic composite adsorbent.
27060639	9	54	theme	aqueous	1558:1564	arg1	solutions					1566:1574	dilute NaOH aqueous solutions	1546:1574	dilute NaOH aqueous solutions then virtually reused with little adsorption capacity loss	1546:1633	This magnetic adsorbent after saturated adsorption could be rapidly separated from water and easily regenerated using dilute NaOH aqueous solutions then virtually reused with little adsorption capacity loss.
27060639	1	55	theme	adsorbent	229:237	arg1	composite					219:227	A quaternary ammonium salt modified chitosan magnetic composite adsorbent	165:237	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM)	165:250	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	1	55	theme	adsorbent	229:237	arg1	CS-CTA-MCM					240:249	CS-CTA-MCM	240:249	CS-CTA-MCM	240:249	A quaternary ammonium salt modified chitosan magnetic composite adsorbent (CS-CTA-MCM) was prepared by combination of Fe3O4 nanoparticles.
27060639	0	56	theme	chromium	47:54	arg1	adsorption					10:19	Efficient adsorption	0:19	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt	0:115	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt modified chitosan magnetic composite adsorbent.
27060639	0	57	theme	adsorbent	154:162	arg1	chitosan					126:133	chitosan	126:133	chitosan magnetic composite adsorbent	126:162	Efficient adsorption of both methyl orange and chromium from their aqueous mixtures using a quaternary ammonium salt modified chitosan magnetic composite adsorbent.
27060639	8	58	theme	Cr	1341:1342	arg1	adsorptions					1326:1336	adsorptions	1326:1336	adsorptions of Cr(VI) and MO by CS-CTA-MCM	1326:1367	The adsorption isotherms and kinetics indicate that adsorptions of Cr(VI) and MO by CS-CTA-MCM both follow a homogeneous monolayer chemisorption process.
27060639	3	59	from	removal	476:482	arg1	systems					577:583	respective single and binary systems	548:583	respective single and binary systems	548:583	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	60	theme	Cr	487:488	arg1	removal					476:482	the removal	472:482	the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems	472:583	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	61	theme	respective	548:557	arg1	systems					577:583	respective single and binary systems	548:583	respective single and binary systems	548:583	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	7	62	theme	pH	1259:1260	arg1	conditions					1262:1271	suitable pH conditions	1250:1271	suitable pH conditions	1250:1271	Thus chromium could be efficient removal together with MO at suitable pH conditions.
27060639	2	63	theme	molecular	353:361	arg1	structure					363:371	molecular structure	353:371	molecular structure	353:371	Various techniques were used to characterize the molecular structure, surface morphology, and magnetic feature of this composite adsorbent.
27060639	5	64	theme	metal	958:962	arg1	ions					964:967	metal ions	958:967	metal ions in their aqueous mixture	958:992	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	4	65	theme	salt	784:787	arg1	groups					789:794	the additional quaternary ammonium salt groups	749:794	the additional quaternary ammonium salt groups	749:794	Compared with chitosan magnetic adsorbent (CS-MCM) without modification, CS-CTA-MCM shows evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups.
27060639	5	66	dep	causing	889:895	arg1	VI					885:886	VI	885:886	VI	885:886	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	4	67	theme	ammonium	775:782	arg1	groups					789:794	the additional quaternary ammonium salt groups	749:794	the additional quaternary ammonium salt groups	749:794	Compared with chitosan magnetic adsorbent (CS-MCM) without modification, CS-CTA-MCM shows evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups.
27060639	4	68	theme	adsorption	695:704	arg1	capacities					706:715	evidently improved adsorption capacities	676:715	evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups	676:794	Compared with chitosan magnetic adsorbent (CS-MCM) without modification, CS-CTA-MCM shows evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups.
27060639	2	69	used	used	328:331	arg2	techniques					312:321	Various techniques	304:321	Various techniques	304:321	Various techniques were used to characterize the molecular structure, surface morphology, and magnetic feature of this composite adsorbent.
27060639	6	70	dep	improved	1061:1068	arg1	VI					1033:1034	VI	1033:1034	VI	1033:1034	However, at weak acidic solutions, Cr(VI) adsorption is evidently improved due to more efficient Cr(VI) forms, i.e. dichromate and monovalent chromate, binding to this chitosan-based adsorbent.
27060639	4	71	theme	magnetic	609:616	arg1	CS-MCM					629:634	CS-MCM	629:634	CS-MCM	629:634	Compared with chitosan magnetic adsorbent (CS-MCM) without modification, CS-CTA-MCM shows evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups.
27060639	4	71	theme	magnetic	609:616	arg1	chitosan					600:607	chitosan magnetic adsorbent	600:626	chitosan magnetic adsorbent (CS-MCM) without modification	600:656	Compared with chitosan magnetic adsorbent (CS-MCM) without modification, CS-CTA-MCM shows evidently improved adsorption capacities for both pollutants ascribed to the additional quaternary ammonium salt groups.
27060639	3	72	theme	binary	570:575	arg1	systems					577:583	respective single and binary systems	548:583	respective single and binary systems	548:583	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	5	73	theme	equilibrium	821:831	arg1	study					833:837	the adsorption equilibrium study	806:837	the adsorption equilibrium study	806:837	Based on the adsorption equilibrium study, MO bears more affinity to CS-CTA-MCM than Cr(VI) causing a considerable extent of preferential adsorption of dye over metal ions in their aqueous mixture.
27060639	8	74	dep	Cr	1341:1342	arg1	VI					1344:1345	VI	1344:1345	VI	1344:1345	The adsorption isotherms and kinetics indicate that adsorptions of Cr(VI) and MO by CS-CTA-MCM both follow a homogeneous monolayer chemisorption process.
27060639	3	75	from	water	539:543	arg1	removal					476:482	the removal	472:482	the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems	472:583	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	3	76	dep	Cr	487:488	arg1	VI					490:491	VI	490:491	VI	490:491	CS-CTA-MCM was employed for the removal of Cr(VI) and methyl orange (MO), an anionic dye, from water in respective single and binary systems.
27060639	6	77	theme	monovalent	1126:1135	arg1	Cr					1092:1093	more efficient Cr(VI) forms	1077:1103	more efficient Cr(VI) forms	1077:1103	However, at weak acidic solutions, Cr(VI) adsorption is evidently improved due to more efficient Cr(VI) forms, i.e. dichromate and monovalent chromate, binding to this chitosan-based adsorbent.
27060639	6	77	theme	monovalent	1126:1135	arg1	chromate					1137:1144	monovalent chromate	1126:1144	monovalent chromate	1126:1144	However, at weak acidic solutions, Cr(VI) adsorption is evidently improved due to more efficient Cr(VI) forms, i.e. dichromate and monovalent chromate, binding to this chitosan-based adsorbent.
27060639	9	78	theme	dilute	1546:1551	arg1	solutions					1566:1574	dilute NaOH aqueous solutions	1546:1574	dilute NaOH aqueous solutions then virtually reused with little adsorption capacity loss	1546:1633	This magnetic adsorbent after saturated adsorption could be rapidly separated from water and easily regenerated using dilute NaOH aqueous solutions then virtually reused with little adsorption capacity loss.
25929025	1	0	theme	raw	322:324	arg1	material					326:333	raw material	322:333	raw material	322:333	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	1	1	with	milk	220:223	arg1	inulin					239:244	inulin	239:244	inulin (Jerusalem artichoke extract)	239:274	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	1	1	with	milk	220:223	arg1	extract					267:273	Jerusalem artichoke extract	247:273	Jerusalem artichoke extract	247:273	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	4	2	theme	drink	738:742	arg1	characteristics					715:729	Physical-chemical and microbiological characteristics	677:729	Physical-chemical and microbiological characteristics of the drink	677:742	Physical-chemical and microbiological characteristics of the drink have been defined during storage.
25929025	6	3	theme	Jerusalem	1047:1055	arg1	extract					1067:1073	Jerusalem artichoke extract	1047:1073	Jerusalem artichoke extract	1047:1073	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	5	4	theme	nutrient	810:817	arg1	composition					819:829	the nutrient composition	806:829	the nutrient composition of fermented dairy product	806:856	The possibility to optimize the nutrient composition of fermented dairy product by means of introducing of Jerusalem artichoke extract into milk-protein base has been demonstrated.
25929025	1	5	theme	material	326:333	arg1	composition					307:317	initial mineral composition	291:317	initial mineral composition of raw material	291:333	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	5	6	theme	fermented	834:842	arg1	product					850:856	fermented dairy product	834:856	fermented dairy product	834:856	The possibility to optimize the nutrient composition of fermented dairy product by means of introducing of Jerusalem artichoke extract into milk-protein base has been demonstrated.
25929025	8	7	theme	dairy	1439:1443	arg1	drink					1445:1449	Designed fermented dairy drink	1420:1449	Designed fermented dairy drink supplemented with natural plant ingredient	1420:1492	Designed fermented dairy drink supplemented with natural plant ingredient possesses increased antioxidant activity and may be recommended for mass consumption without any limitations.
25929025	3	8	from	derivatives	503:513	arg1	form					522:525	the form	518:525	the form of syrups and extracts	518:548	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	3	9	theme	syrups	530:535	arg1	form					522:525	the form	518:525	the form of syrups and extracts	518:548	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	4	10	theme	Physical-chemical	677:693	arg1	characteristics					715:729	Physical-chemical and microbiological characteristics	677:729	Physical-chemical and microbiological characteristics of the drink	677:742	Physical-chemical and microbiological characteristics of the drink have been defined during storage.
25929025	6	11	theme	vitamins	1196:1203	arg1	B1					1205:1206	vitamins B1	1196:1206	vitamins B1	1196:1206	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	6	12	theme	drink	1027:1031	arg1	consumption					987:997	consumption	987:997	consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract	987:1073	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	7	13	dep	vitamins	1341:1348	arg1	vitamins					1341:1348	vitamins B1, B2 and B6	1341:1362	vitamins B1, B2 and B6	1341:1362	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	7	13	dep	vitamins	1341:1348	arg1	B2					1354:1355	B2	1354:1355	B2	1354:1355	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	7	13	dep	vitamins	1341:1348	arg1	B1					1350:1351	B1	1350:1351	B1	1350:1351	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	7	13	dep	vitamins	1341:1348	arg1	B6					1361:1362	B6	1361:1362	B6	1361:1362	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	1	14	theme	Jerusalem	247:255	arg1	inulin					239:244	inulin	239:244	inulin (Jerusalem artichoke extract)	239:274	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	1	14	theme	Jerusalem	247:255	arg1	extract					267:273	Jerusalem artichoke extract	247:273	Jerusalem artichoke extract	247:273	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	3	15	with	microflora	600:609	arg1	activity					625:632	maximum activity	617:632	maximum activity observed with Jerusalem artichoke extract	617:674	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	8	16	theme	fermented	1429:1437	arg1	drink					1445:1449	Designed fermented dairy drink	1420:1449	Designed fermented dairy drink supplemented with natural plant ingredient	1420:1492	Designed fermented dairy drink supplemented with natural plant ingredient possesses increased antioxidant activity and may be recommended for mass consumption without any limitations.
25929025	5	17	theme	milk-protein	918:929	arg1	base					931:934	milk-protein base	918:934	milk-protein base	918:934	The possibility to optimize the nutrient composition of fermented dairy product by means of introducing of Jerusalem artichoke extract into milk-protein base has been demonstrated.
25929025	2	18	theme	organoleptic	389:400	arg1	properties					402:411	optimal organoleptic properties	381:411	optimal organoleptic properties of the drink	381:424	The starters ascertaining optimal organoleptic properties of the drink have been selected.
25929025	5	19	theme	dairy	844:848	arg1	product					850:856	fermented dairy product	834:856	fermented dairy product	834:856	The possibility to optimize the nutrient composition of fermented dairy product by means of introducing of Jerusalem artichoke extract into milk-protein base has been demonstrated.
25929025	8	20	theme	Designed	1420:1427	arg1	drink					1445:1449	Designed fermented dairy drink	1420:1449	Designed fermented dairy drink supplemented with natural plant ingredient	1420:1492	Designed fermented dairy drink supplemented with natural plant ingredient possesses increased antioxidant activity and may be recommended for mass consumption without any limitations.
25929025	2	21	theme	optimal	381:387	arg1	properties					402:411	optimal organoleptic properties	381:411	optimal organoleptic properties of the drink	381:424	The starters ascertaining optimal organoleptic properties of the drink have been selected.
25929025	5	22	theme	product	850:856	arg1	composition					819:829	the nutrient composition	806:829	the nutrient composition of fermented dairy product	806:856	The possibility to optimize the nutrient composition of fermented dairy product by means of introducing of Jerusalem artichoke extract into milk-protein base has been demonstrated.
25929025	6	23	theme	dairy	1021:1025	arg1	drink					1027:1031	fermented dairy drink	1011:1031	fermented dairy drink enriched with Jerusalem artichoke extract	1011:1073	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	3	24	theme	fermentative	560:571	arg1	processes					573:581	fermentative processes	560:581	fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract	560:674	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	8	25	theme	antioxidant	1514:1524	arg1	activity					1526:1533	increased antioxidant activity	1504:1533	increased antioxidant activity	1504:1533	Designed fermented dairy drink supplemented with natural plant ingredient possesses increased antioxidant activity and may be recommended for mass consumption without any limitations.
25929025	4	26	theme	microbiological	699:713	arg1	characteristics					715:729	Physical-chemical and microbiological characteristics	677:729	Physical-chemical and microbiological characteristics of the drink	677:742	Physical-chemical and microbiological characteristics of the drink have been defined during storage.
25929025	0	27	theme	[Biotechnological	0:16	arg1	optimization					18:29	[Biotechnological optimization	0:29	[Biotechnological optimization of nutrient composition of fermented dairy drink	0:78	[Biotechnological optimization of nutrient composition of fermented dairy drink].
25929025	1	28	theme	artichoke	257:265	arg1	inulin					239:244	inulin	239:244	inulin (Jerusalem artichoke extract)	239:274	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	1	28	theme	artichoke	257:265	arg1	extract					267:273	Jerusalem artichoke extract	247:273	Jerusalem artichoke extract	247:273	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	3	29	from	artichoke	485:493	arg1	form					522:525	the form	518:525	the form of syrups and extracts	518:548	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	6	30	dep	physiological	1108:1120	arg1	needs					1122:1126	needs	1122:1126	needs	1122:1126	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	6	30	dep	physiological	1108:1120	arg1	allowance--RDA					1147:1160	allowance--RDA	1147:1160	allowance--RDA	1147:1160	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	6	31	from	months	1186:1191	arg1	B6					1216:1217	B6	1216:1217	B6	1216:1217	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	6	31	from	months	1186:1191	arg1	B2					1209:1210	B2	1209:1210	B2	1209:1210	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	6	31	from	months	1186:1191	arg1	B1					1205:1206	vitamins B1	1196:1206	vitamins B1	1196:1206	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	6	31	from	months	1186:1191	arg1	babies					1167:1172	babies	1167:1172	babies from 0 to 3 months in vitamins B1, B2 and B6	1167:1217	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	1	32	theme	new	177:179	arg1	drink					197:201	the new fermented dairy drink	173:201	the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material	173:333	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	0	33	theme	nutrient	34:41	arg1	composition					43:53	nutrient composition	34:53	nutrient composition of fermented dairy drink	34:78	[Biotechnological optimization of nutrient composition of fermented dairy drink].
25929025	6	34	theme	fermented	1011:1019	arg1	drink					1027:1031	fermented dairy drink	1011:1031	fermented dairy drink enriched with Jerusalem artichoke extract	1011:1073	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	7	35	theme	fermented	1308:1316	arg1	beverage					1318:1325	fermented beverage	1308:1325	fermented beverage	1308:1325	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	3	36	theme	extracts	541:548	arg1	form					522:525	the form	518:525	the form of syrups and extracts	518:548	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	7	37	theme	beverage	1318:1325	arg1	g					1303:1303	250 g	1299:1303	250 g of fermented beverage	1299:1325	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	2	38	theme	drink	420:424	arg1	properties					402:411	optimal organoleptic properties	381:411	optimal organoleptic properties of the drink	381:424	The starters ascertaining optimal organoleptic properties of the drink have been selected.
25929025	1	39	with	whey	229:232	arg1	inulin					239:244	inulin	239:244	inulin (Jerusalem artichoke extract)	239:274	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	1	39	with	whey	229:232	arg1	extract					267:273	Jerusalem artichoke extract	247:273	Jerusalem artichoke extract	247:273	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	7	40	dep	macronutrients	1385:1398	arg1	%					1417:1417	Ca-by 25-35%	1406:1417	Ca-by 25-35%	1406:1417	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	7	40	dep	macronutrients	1385:1398	arg1	K					1403:1403	K	1403:1403	K	1403:1403	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	7	40	dep	macronutrients	1385:1398	arg1	P					1400:1400	P	1400:1400	P	1400:1400	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	8	41	theme	mass	1562:1565	arg1	consumption					1567:1577	mass consumption	1562:1577	mass consumption without any limitations	1562:1601	Designed fermented dairy drink supplemented with natural plant ingredient possesses increased antioxidant activity and may be recommended for mass consumption without any limitations.
25929025	3	42	theme	artichoke	658:666	arg1	extract					668:674	Jerusalem artichoke extract	648:674	Jerusalem artichoke extract	648:674	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	6	43	dep	minerals	1236:1243	arg1	minerals					1236:1243	minerals P, K, and Ca	1236:1256	minerals P, K, and Ca	1236:1256	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	6	43	dep	minerals	1236:1243	arg1	K					1248:1248	K	1248:1248	K	1248:1248	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	6	43	dep	minerals	1236:1243	arg1	Ca					1255:1256	Ca	1255:1256	Ca	1255:1256	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	6	43	dep	minerals	1236:1243	arg1	P					1245:1245	P	1245:1245	P	1245:1245	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	1	44	theme	dairy	191:195	arg1	drink					197:201	the new fermented dairy drink	173:201	the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material	173:333	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	6	45	theme	g	1006:1006	arg1	consumption					987:997	consumption	987:997	consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract	987:1073	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	5	46	theme	Jerusalem	885:893	arg1	extract					905:911	Jerusalem artichoke extract	885:911	Jerusalem artichoke extract	885:911	The possibility to optimize the nutrient composition of fermented dairy product by means of introducing of Jerusalem artichoke extract into milk-protein base has been demonstrated.
25929025	3	47	theme	microflora	600:609	arg1	processes					573:581	fermentative processes	560:581	fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract	560:674	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	1	48	theme	drink	197:201	arg1	technology					159:168	technology	159:168	technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material	159:333	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	0	49	theme	composition	43:53	arg1	optimization					18:29	[Biotechnological optimization	0:29	[Biotechnological optimization of nutrient composition of fermented dairy drink	0:78	[Biotechnological optimization of nutrient composition of fermented dairy drink].
25929025	8	50	theme	natural	1469:1475	arg1	ingredient					1483:1492	natural plant ingredient	1469:1492	natural plant ingredient	1469:1492	Designed fermented dairy drink supplemented with natural plant ingredient possesses increased antioxidant activity and may be recommended for mass consumption without any limitations.
25929025	5	51	theme	artichoke	895:903	arg1	extract					905:911	Jerusalem artichoke extract	885:911	Jerusalem artichoke extract	885:911	The possibility to optimize the nutrient composition of fermented dairy product by means of introducing of Jerusalem artichoke extract into milk-protein base has been demonstrated.
25929025	0	52	theme	dairy	68:72	arg1	drink					74:78	fermented dairy drink	58:78	fermented dairy drink	58:78	[Biotechnological optimization of nutrient composition of fermented dairy drink].
25929025	1	53	theme	initial	291:297	arg1	composition					307:317	initial mineral composition	291:317	initial mineral composition of raw material	291:333	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	3	54	theme	Jerusalem	648:656	arg1	extract					668:674	Jerusalem artichoke extract	648:674	Jerusalem artichoke extract	648:674	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	8	55	contain	possesses	1494:1502	arg1	drink					1445:1449	Designed fermented dairy drink	1420:1449	Designed fermented dairy drink supplemented with natural plant ingredient	1420:1492	Designed fermented dairy drink supplemented with natural plant ingredient possesses increased antioxidant activity and may be recommended for mass consumption without any limitations.
25929025	8	55	contain	possesses	1494:1502	arg2	activity					1526:1533	increased antioxidant activity	1504:1533	increased antioxidant activity	1504:1533	Designed fermented dairy drink supplemented with natural plant ingredient possesses increased antioxidant activity and may be recommended for mass consumption without any limitations.
25929025	0	56	theme	fermented	58:66	arg1	drink					74:78	fermented dairy drink	58:78	fermented dairy drink	58:78	[Biotechnological optimization of nutrient composition of fermented dairy drink].
25929025	1	57	theme	mineral	299:305	arg1	composition					307:317	initial mineral composition	291:317	initial mineral composition of raw material	291:333	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	3	58	theme	technological	586:598	arg1	microflora					600:609	technological microflora	586:609	technological microflora	586:609	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	7	59	from	RDA	1333:1335	arg1	macronutrients					1385:1398	the macronutrients P, K, Ca-by 25-35%	1381:1417	the macronutrients P, K, Ca-by 25-35%	1381:1417	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	8	60	theme	plant	1477:1481	arg1	ingredient					1483:1492	natural plant ingredient	1469:1492	natural plant ingredient	1469:1492	Designed fermented dairy drink supplemented with natural plant ingredient possesses increased antioxidant activity and may be recommended for mass consumption without any limitations.
25929025	7	61	theme	Ca-by	1406:1410	arg1	%					1417:1417	Ca-by 25-35%	1406:1417	Ca-by 25-35%	1406:1417	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	7	61	theme	Ca-by	1406:1410	arg1	P					1400:1400	P	1400:1400	P	1400:1400	For adults receiving 250 g of fermented beverage meets RDA for vitamins B1, B2 and B6 by 10-19% and in the macronutrients P, K, Ca-by 25-35%.
25929025	1	62	dep	drink	197:201	arg1	optimizing					280:289	optimizing	280:289	optimizing initial mineral composition of raw material	280:333	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	1	62	dep	drink	197:201	arg1	containing					203:212	containing	203:212	containing whole milk and whey with inulin (Jerusalem artichoke extract)	203:274	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	1	63	theme	whole	214:218	arg1	milk					220:223	whole milk	214:223	whole milk	214:223	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	3	64	theme	Jerusalem	475:483	arg1	artichoke					485:493	Jerusalem artichoke	475:493	Jerusalem artichoke	475:493	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	1	65	theme	fermented	181:189	arg1	drink					197:201	the new fermented dairy drink	173:201	the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material	173:333	The receipt based on the results of carried out studies is substantiated and technology of the new fermented dairy drink containing whole milk and whey with inulin (Jerusalem artichoke extract) and optimizing initial mineral composition of raw material has been developed.
25929025	6	66	dep	3	1184:1184	arg1	to					1181:1182	to	1181:1182	to	1181:1182	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
25929025	0	67	theme	drink	74:78	arg1	composition					43:53	nutrient composition	34:53	nutrient composition of fermented dairy drink	34:78	[Biotechnological optimization of nutrient composition of fermented dairy drink].
25929025	3	68	theme	maximum	617:623	arg1	activity					625:632	maximum activity	617:632	maximum activity observed with Jerusalem artichoke extract	617:674	It has been established that Jerusalem artichoke and its derivatives in the form of syrups and extracts stimulate fermentative processes of technological microflora, with maximum activity observed with Jerusalem artichoke extract.
25929025	8	69	theme	increased	1504:1512	arg1	activity					1526:1533	increased antioxidant activity	1504:1533	increased antioxidant activity	1504:1533	Designed fermented dairy drink supplemented with natural plant ingredient possesses increased antioxidant activity and may be recommended for mass consumption without any limitations.
25929025	6	70	theme	artichoke	1057:1065	arg1	extract					1067:1073	Jerusalem artichoke extract	1047:1073	Jerusalem artichoke extract	1047:1073	It has been calculated that consumption of 100 g of fermented dairy drink enriched with Jerusalem artichoke extract makes it possible to satisfy the physiological needs (recommended daily allowance--RDA) for babies from 0 to 3 months in vitamins B1, B2 and B6 by 25-35% and in minerals P, K, and Ca by 20, 68, 34, 26%.
28008083	0	0	theme	leaf	62:65	arg1	Δ18O					54:57	Δ18O	54:57	Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient	54:141	Infidelity in the outback: climate signal recorded in Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient.
28008083	8	1	theme	cellulose	1414:1422	arg1	use					1397:1399	the use	1393:1399	the use of tree-ring cellulose as a climate proxy	1393:1441	We consider potential mechanisms that could explain the 'damping' of a climatic signal within wood cellulose and consider the implication and limitations on the use of tree-ring cellulose as a climate proxy.
28008083	7	2	theme	source	1131:1136	arg1	water					1138:1142	altered source water	1123:1142	altered source water	1123:1142	However, once the effect of altered source water was considered we found wood cellulose to show no trend across this environmental gradient.
28008083	4	3	from	roles	655:659	arg1	composition					719:729	the isotopic composition	706:729	the isotopic composition of leaf and wood cellulose within the same plant	706:778	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	5	4	theme	xylem/source	844:855	arg1	water					857:861	xylem/source water	844:861	xylem/source water of eucalypts	844:874	Here, we study both leaf and branch wood cellulose, as well as xylem/source water of eucalypts across a 900 km aridity gradient in NE Australia.
28008083	3	5	theme	cellulose	474:482	arg1	composition					493:503	cellulose isotopic composition	474:503	cellulose isotopic composition	474:503	However, the mechanisms by which cellulose isotopic composition reflect environmental conditions are complex, and may vary between leaf and woody tissues.
28008083	0	6	from	Infidelity	0:9	arg1	outback					18:24	the outback	14:24	the outback	14:24	Infidelity in the outback: climate signal recorded in Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient.
28008083	2	7	theme	isotopic	258:265	arg1	signature					267:275	This isotopic signature	253:275	This isotopic signature	253:275	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	6	8	theme	consistent	1059:1068	arg1	trend					1070:1074	a strong and consistent trend	1046:1074	a strong and consistent trend in leaf cellulose	1046:1092	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	8	9	theme	tree-ring	1404:1412	arg1	cellulose					1414:1422	tree-ring cellulose	1404:1422	tree-ring cellulose	1404:1422	We consider potential mechanisms that could explain the 'damping' of a climatic signal within wood cellulose and consider the implication and limitations on the use of tree-ring cellulose as a climate proxy.
28008083	6	10	theme	leaf	1079:1082	arg1	cellulose					1084:1092	leaf cellulose	1079:1092	leaf cellulose	1079:1092	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	7	11	theme	water	1138:1142	arg1	effect					1113:1118	the effect	1109:1118	the effect of altered source water	1109:1142	However, once the effect of altered source water was considered we found wood cellulose to show no trend across this environmental gradient.
28008083	5	12	theme	aridity	892:898	arg1	gradient					900:907	a 900 km aridity gradient	883:907	a 900 km aridity gradient in NE Australia	883:923	Here, we study both leaf and branch wood cellulose, as well as xylem/source water of eucalypts across a 900 km aridity gradient in NE Australia.
28008083	0	13	theme	cellulose	82:90	arg1	Δ18O					54:57	Δ18O	54:57	Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient	54:141	Infidelity in the outback: climate signal recorded in Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient.
28008083	7	14	theme	wood	1168:1171	arg1	cellulose					1173:1181	wood cellulose	1168:1181	wood cellulose	1168:1181	However, once the effect of altered source water was considered we found wood cellulose to show no trend across this environmental gradient.
28008083	8	15	theme	potential	1248:1256	arg1	mechanisms					1258:1267	potential mechanisms	1248:1267	potential mechanisms that could explain the 'damping' of a climatic signal within wood cellulose	1248:1343	We consider potential mechanisms that could explain the 'damping' of a climatic signal within wood cellulose and consider the implication and limitations on the use of tree-ring cellulose as a climate proxy.
28008083	5	16	theme	branch	810:815	arg1	cellulose					822:830	branch wood cellulose	810:830	branch wood cellulose	810:830	Here, we study both leaf and branch wood cellulose, as well as xylem/source water of eucalypts across a 900 km aridity gradient in NE Australia.
28008083	6	17	theme	average	1000:1006	arg1	humidity					1017:1024	average relative humidity	1000:1024	average relative humidity of 33-70%	1000:1034	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	0	18	theme	branch	75:80	arg1	cellulose					82:90	branch cellulose	75:90	leaf but not branch cellulose of eucalypts	62:103	Infidelity in the outback: climate signal recorded in Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient.
28008083	3	19	theme	environmental	513:525	arg1	conditions					527:536	environmental conditions	513:536	environmental conditions	513:536	However, the mechanisms by which cellulose isotopic composition reflect environmental conditions are complex, and may vary between leaf and woody tissues.
28008083	1	20	theme	isotopic	148:155	arg1	composition					157:167	The isotopic composition	144:167	The isotopic composition of leaf water in terrestrial plants	144:203	The isotopic composition of leaf water in terrestrial plants is highly dependent upon a plant's environment.
28008083	1	20	theme	isotopic	148:155	arg1	dependent					215:223	dependent	215:223	dependent	215:223	The isotopic composition of leaf water in terrestrial plants is highly dependent upon a plant's environment.
28008083	6	21	theme	average	952:958	arg1	precipitation					967:979	average annual precipitation	952:979	average annual precipitation of 235-1400 mm	952:994	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	5	22	theme	eucalypts	866:874	arg1	cellulose					822:830	branch wood cellulose	810:830	branch wood cellulose	810:830	Here, we study both leaf and branch wood cellulose, as well as xylem/source water of eucalypts across a 900 km aridity gradient in NE Australia.
28008083	5	22	theme	eucalypts	866:874	arg1	water					857:861	xylem/source water	844:861	xylem/source water of eucalypts	844:874	Here, we study both leaf and branch wood cellulose, as well as xylem/source water of eucalypts across a 900 km aridity gradient in NE Australia.
28008083	5	22	theme	eucalypts	866:874	arg1	leaf					801:804	leaf	801:804	leaf	801:804	Here, we study both leaf and branch wood cellulose, as well as xylem/source water of eucalypts across a 900 km aridity gradient in NE Australia.
28008083	6	23	theme	mm	993:994	arg1	precipitation					967:979	average annual precipitation	952:979	average annual precipitation of 235-1400 mm	952:994	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	6	23	theme	mm	993:994	arg1	humidity					1017:1024	average relative humidity	1000:1024	average relative humidity of 33-70%	1000:1034	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	2	24	theme	biomarkers	360:369	arg1	paleo					413:417	sensitive paleo	403:417	sensitive paleo	403:417	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	2	24	theme	biomarkers	360:369	arg1	proxies					432:438	climatic proxies	423:438	climatic proxies	423:438	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	2	24	theme	biomarkers	360:369	arg1	composition					345:355	the isotopic composition	332:355	the isotopic composition of biomarkers such as cellulose	332:387	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	8	25	theme	climate	1429:1435	arg1	proxy					1437:1441	a climate proxy	1427:1441	a climate proxy	1427:1441	We consider potential mechanisms that could explain the 'damping' of a climatic signal within wood cellulose and consider the implication and limitations on the use of tree-ring cellulose as a climate proxy.
28008083	4	26	theme	water	697:701	arg1	roles					655:659	the relative roles	642:659	the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant	642:778	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	5	27	theme	wood	817:820	arg1	cellulose					822:830	branch wood cellulose	810:830	branch wood cellulose	810:830	Here, we study both leaf and branch wood cellulose, as well as xylem/source water of eucalypts across a 900 km aridity gradient in NE Australia.
28008083	2	28	theme	sensitive	403:411	arg1	paleo					413:417	sensitive paleo	403:417	sensitive paleo	403:417	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	2	28	theme	sensitive	403:411	arg1	composition					345:355	the isotopic composition	332:355	the isotopic composition of biomarkers such as cellulose	332:387	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	8	29	theme	signal	1316:1321	arg1	damping					1293:1299	the 'damping'	1288:1300	the 'damping' of a climatic signal within wood cellulose	1288:1343	We consider potential mechanisms that could explain the 'damping' of a climatic signal within wood cellulose and consider the implication and limitations on the use of tree-ring cellulose as a climate proxy.
28008083	0	30	theme	eucalypts	95:103	arg1	cellulose					82:90	branch cellulose	75:90	leaf but not branch cellulose of eucalypts	62:103	Infidelity in the outback: climate signal recorded in Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient.
28008083	4	31	theme	isotopic	710:717	arg1	composition					719:729	the isotopic composition	706:729	the isotopic composition of leaf and wood cellulose within the same plant	706:778	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	1	32	theme	leaf	172:175	arg1	water					177:181	leaf water	172:181	leaf water	172:181	The isotopic composition of leaf water in terrestrial plants is highly dependent upon a plant's environment.
28008083	8	33	from	limitations	1378:1388	arg1	use					1397:1399	the use	1393:1399	the use of tree-ring cellulose as a climate proxy	1393:1441	We consider potential mechanisms that could explain the 'damping' of a climatic signal within wood cellulose and consider the implication and limitations on the use of tree-ring cellulose as a climate proxy.
28008083	8	34	theme	wood	1330:1333	arg1	cellulose					1335:1343	wood cellulose	1330:1343	wood cellulose	1330:1343	We consider potential mechanisms that could explain the 'damping' of a climatic signal within wood cellulose and consider the implication and limitations on the use of tree-ring cellulose as a climate proxy.
28008083	6	35	theme	relative	1008:1015	arg1	humidity					1017:1024	average relative humidity	1000:1024	average relative humidity of 33-70%	1000:1034	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	1	36	theme	water	177:181	arg1	composition					157:167	The isotopic composition	144:167	The isotopic composition of leaf water in terrestrial plants	144:203	The isotopic composition of leaf water in terrestrial plants is highly dependent upon a plant's environment.
28008083	1	36	theme	water	177:181	arg1	dependent					215:223	dependent	215:223	dependent	215:223	The isotopic composition of leaf water in terrestrial plants is highly dependent upon a plant's environment.
28008083	0	37	theme	climate	27:33	arg1	signal					35:40	climate signal	27:40	Infidelity in the outback: climate signal recorded in Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient.	0:142	Infidelity in the outback: climate signal recorded in Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient.
28008083	5	38	theme	km	889:890	arg1	gradient					900:907	a 900 km aridity gradient	883:907	a 900 km aridity gradient in NE Australia	883:923	Here, we study both leaf and branch wood cellulose, as well as xylem/source water of eucalypts across a 900 km aridity gradient in NE Australia.
28008083	6	39	theme	annual	960:965	arg1	precipitation					967:979	average annual precipitation	952:979	average annual precipitation of 235-1400 mm	952:994	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	4	40	theme	relative	646:653	arg1	roles					655:659	the relative roles	642:659	the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant	642:778	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	7	41	theme	environmental	1212:1224	arg1	gradient					1226:1233	this environmental gradient	1207:1233	this environmental gradient	1207:1233	However, once the effect of altered source water was considered we found wood cellulose to show no trend across this environmental gradient.
28008083	4	42	theme	same	769:772	arg1	plant					774:778	the same plant	765:778	the same plant	765:778	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	1	43	theme	terrestrial	186:196	arg1	plants					198:203	terrestrial plants	186:203	terrestrial plants	186:203	The isotopic composition of leaf water in terrestrial plants is highly dependent upon a plant's environment.
28008083	0	44	theme	aridity	126:132	arg1	gradient					134:141	an Australian aridity gradient	112:141	an Australian aridity gradient	112:141	Infidelity in the outback: climate signal recorded in Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient.
28008083	8	45	dep	implication	1362:1372	arg1	the					1358:1360	the	1358:1360	the	1358:1360	We consider potential mechanisms that could explain the 'damping' of a climatic signal within wood cellulose and consider the implication and limitations on the use of tree-ring cellulose as a climate proxy.
28008083	6	46	from	trend	1070:1074	arg1	cellulose					1084:1092	leaf cellulose	1079:1092	leaf cellulose	1079:1092	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	4	47	theme	enrichment	675:684	arg1	roles					655:659	the relative roles	642:659	the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant	642:778	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	2	48	theme	climatic	423:430	arg1	proxies					432:438	climatic proxies	423:438	climatic proxies	423:438	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	2	48	theme	climatic	423:430	arg1	composition					345:355	the isotopic composition	332:355	the isotopic composition of biomarkers such as cellulose	332:387	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	0	49	theme	Australian	115:124	arg1	gradient					134:141	an Australian aridity gradient	112:141	an Australian aridity gradient	112:141	Infidelity in the outback: climate signal recorded in Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient.
28008083	4	50	theme	leaf-water	664:673	arg1	enrichment					675:684	leaf-water enrichment	664:684	leaf-water enrichment	664:684	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	8	51	theme	climatic	1307:1314	arg1	signal					1316:1321	a climatic signal	1305:1321	a climatic signal within wood cellulose	1305:1343	We consider potential mechanisms that could explain the 'damping' of a climatic signal within wood cellulose and consider the implication and limitations on the use of tree-ring cellulose as a climate proxy.
28008083	5	52	theme	NE	912:913	arg1	Australia					915:923	NE Australia	912:923	NE Australia	912:923	Here, we study both leaf and branch wood cellulose, as well as xylem/source water of eucalypts across a 900 km aridity gradient in NE Australia.
28008083	4	53	theme	cellulose	748:756	arg1	composition					719:729	the isotopic composition	706:729	the isotopic composition of leaf and wood cellulose within the same plant	706:778	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	2	54	used	used	395:398	arg2	composition					345:355	the isotopic composition	332:355	the isotopic composition of biomarkers such as cellulose	332:387	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	2	54	used	used	395:398	arg2	proxies					432:438	climatic proxies	423:438	climatic proxies	423:438	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	2	54	used	used	395:398	arg2	paleo					413:417	sensitive paleo	403:417	sensitive paleo	403:417	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	2	55	theme	organic	304:310	arg1	molecules					312:320	organic molecules	304:320	organic molecules	304:320	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	6	56	theme	strong	1048:1053	arg1	trend					1070:1074	a strong and consistent trend	1046:1074	a strong and consistent trend in leaf cellulose	1046:1092	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	4	57	theme	leaf	734:737	arg1	composition					719:729	the isotopic composition	706:729	the isotopic composition of leaf and wood cellulose within the same plant	706:778	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	4	58	theme	wood	743:746	arg1	cellulose					748:756	wood cellulose	743:756	wood cellulose	743:756	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	1	59	from	composition	157:167	arg1	plants					198:203	terrestrial plants	186:203	terrestrial plants	186:203	The isotopic composition of leaf water in terrestrial plants is highly dependent upon a plant's environment.
28008083	6	60	theme	%	1034:1034	arg1	precipitation					967:979	average annual precipitation	952:979	average annual precipitation of 235-1400 mm	952:994	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	6	60	theme	%	1034:1034	arg1	humidity					1017:1024	average relative humidity	1000:1024	average relative humidity of 33-70%	1000:1034	Across 11 sites, spanning average annual precipitation of 235-1400 mm and average relative humidity of 33-70%, we found a strong and consistent trend in leaf cellulose.
28008083	4	61	theme	source	690:695	arg1	water					697:701	source water	690:701	source water	690:701	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	4	62	theme	empirical	608:616	arg1	tests					618:622	few empirical tests	604:622	few empirical tests	604:622	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	3	63	theme	woody	581:585	arg1	tissues					587:593	woody tissues	581:593	woody tissues	581:593	However, the mechanisms by which cellulose isotopic composition reflect environmental conditions are complex, and may vary between leaf and woody tissues.
28008083	4	64	theme	few	604:606	arg1	tests					618:622	few empirical tests	604:622	few empirical tests	604:622	To date few empirical tests have been made on the relative roles of leaf-water enrichment and source water on the isotopic composition of leaf and wood cellulose within the same plant.
28008083	3	65	theme	isotopic	484:491	arg1	composition					493:503	cellulose isotopic composition	474:503	cellulose isotopic composition	474:503	However, the mechanisms by which cellulose isotopic composition reflect environmental conditions are complex, and may vary between leaf and woody tissues.
28008083	7	66	theme	altered	1123:1129	arg1	water					1138:1142	altered source water	1123:1142	altered source water	1123:1142	However, once the effect of altered source water was considered we found wood cellulose to show no trend across this environmental gradient.
28008083	0	67	dep	Infidelity	0:9	arg1	signal					35:40	climate signal	27:40	Infidelity in the outback: climate signal recorded in Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient.	0:142	Infidelity in the outback: climate signal recorded in Δ18O of leaf but not branch cellulose of eucalypts across an Australian aridity gradient.
28008083	2	68	theme	isotopic	336:343	arg1	paleo					413:417	sensitive paleo	403:417	sensitive paleo	403:417	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	2	68	theme	isotopic	336:343	arg1	proxies					432:438	climatic proxies	423:438	climatic proxies	423:438	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	2	68	theme	isotopic	336:343	arg1	composition					345:355	the isotopic composition	332:355	the isotopic composition of biomarkers such as cellulose	332:387	This isotopic signature can become integrated into organic molecules, allowing the isotopic composition of biomarkers such as cellulose to be used as sensitive paleo and climatic proxies.
28008083	5	69	from	gradient	900:907	arg1	Australia					915:923	NE Australia	912:923	NE Australia	912:923	Here, we study both leaf and branch wood cellulose, as well as xylem/source water of eucalypts across a 900 km aridity gradient in NE Australia.
28008083	8	70	from	implication	1362:1372	arg1	use					1397:1399	the use	1393:1399	the use of tree-ring cellulose as a climate proxy	1393:1441	We consider potential mechanisms that could explain the 'damping' of a climatic signal within wood cellulose and consider the implication and limitations on the use of tree-ring cellulose as a climate proxy.
29124776	0	0	theme	interrelationship	63:79	arg1	report					53:58	a first report	45:58	Field findings about milk ethanol stability: a first report of interrelationship between α-lactalbumin and lactose.	0:114	Field findings about milk ethanol stability: a first report of interrelationship between α-lactalbumin and lactose.
29124776	2	1	theme	major	462:466	arg1	components					468:477	major components	462:477	major components	462:477	On this basis, we wanted to find out more about milk ethanol stability by studying its relationship with milk protein fractions and others major components.
29124776	8	2	theme	lactose	1228:1234	arg1	synthesis					1236:1244	lactose synthesis	1228:1244	lactose synthesis	1228:1244	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	8	3	from	values	1125:1130	arg1	samples					1157:1163	unstable milk samples	1143:1163	unstable milk samples	1143:1163	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	8	4	dep	CONCLUSION	1104:1113	arg1	related					1174:1180	related	1174:1180	related	1174:1180	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	8	4	dep	CONCLUSION	1104:1113	arg1	values					1125:1130	The lower values	1115:1130	The lower values of α-La in unstable milk samples	1115:1163	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	4	5	theme	stability	670:678	arg1	status					680:685	the ethanol stability status	658:685	the ethanol stability status	658:685	An additional model was used to assess the ethanol stability status as a response to the proteins fractions quantified by electrophoresis.
29124776	4	5	theme	stability	670:678	arg1	response					692:699	a response	690:699	a response to the proteins fractions quantified by electrophoresis	690:755	An additional model was used to assess the ethanol stability status as a response to the proteins fractions quantified by electrophoresis.
29124776	6	6	theme	percentage	941:950	arg1	values					892:897	low values	888:897	low values of lactose content	888:916	Milk ethanol instability increased at low values of lactose content and high values of ash percentage.
29124776	6	6	theme	percentage	941:950	arg1	values					927:932	high values	922:932	high values of ash percentage	922:950	Milk ethanol instability increased at low values of lactose content and high values of ash percentage.
29124776	7	7	dep	higher	1026:1031	arg1	lower					1057:1061	lower	1057:1061	lower	1057:1061	α-Lactalbumin (α-La) was also associated with ethanol stability, and the higher the α-La percentage the lower were the chances of ethanol instability.
29124776	7	7	dep	higher	1026:1031	arg1	percentage					1042:1051	the α-La percentage	1033:1051	the higher the α-La percentage the lower	1022:1061	α-Lactalbumin (α-La) was also associated with ethanol stability, and the higher the α-La percentage the lower were the chances of ethanol instability.
29124776	6	8	theme	ethanol	855:861	arg1	instability					863:873	Milk ethanol instability	850:873	Milk ethanol instability	850:873	Milk ethanol instability increased at low values of lactose content and high values of ash percentage.
29124776	3	9	from	influence	484:492	arg1	stability					525:533	ethanol stability	517:533	ethanol stability	517:533	The influence of milk composition on ethanol stability was assessed through a predictive model comprising 180 individual raw milk samples.
29124776	3	10	theme	ethanol	517:523	arg1	stability					525:533	ethanol stability	517:533	ethanol stability	517:533	The influence of milk composition on ethanol stability was assessed through a predictive model comprising 180 individual raw milk samples.
29124776	6	11	theme	ash	937:939	arg1	percentage					941:950	ash percentage	937:950	ash percentage	937:950	Milk ethanol instability increased at low values of lactose content and high values of ash percentage.
29124776	6	12	theme	Milk	850:853	arg1	instability					863:873	Milk ethanol instability	850:873	Milk ethanol instability	850:873	Milk ethanol instability increased at low values of lactose content and high values of ash percentage.
29124776	2	13	dep	fractions	441:449	arg1	components					468:477	major components	462:477	major components	462:477	On this basis, we wanted to find out more about milk ethanol stability by studying its relationship with milk protein fractions and others major components.
29124776	8	14	theme	key	1249:1251	arg1	component					1253:1261	a key component	1247:1261	a key component for the osmotic balance of milk	1247:1293	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	9	15	theme	ethanol	1366:1372	arg1	stability					1374:1382	ethanol stability	1366:1382	ethanol stability	1366:1382	This is the first field report linking ethanol stability indirectly with α-La.
29124776	3	16	theme	composition	502:512	arg1	influence					484:492	The influence	480:492	The influence of milk composition on ethanol stability	480:533	The influence of milk composition on ethanol stability was assessed through a predictive model comprising 180 individual raw milk samples.
29124776	9	17	theme	field	1345:1349	arg1	report					1351:1356	the first field report	1335:1356	the first field report linking ethanol stability indirectly with α-La	1335:1403	This is the first field report linking ethanol stability indirectly with α-La.
29124776	9	17	theme	field	1345:1349	arg1	This					1327:1330	This	1327:1330	This	1327:1330	This is the first field report linking ethanol stability indirectly with α-La.
29124776	5	18	theme	32	822:823	arg1	%					824:824	%	824:824	%	824:824	RESULTS Of the total samples, 68% were classified as stable and 32% as unstable to alcohol.
29124776	5	18	theme	32	822:823	arg1	%					790:790	68%	788:790	68%	788:790	RESULTS Of the total samples, 68% were classified as stable and 32% as unstable to alcohol.
29124776	8	19	theme	milk	1152:1155	arg1	samples					1157:1163	unstable milk samples	1143:1163	unstable milk samples	1143:1163	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	7	20	theme	α-La	1037:1040	arg1	percentage					1042:1051	the α-La percentage	1033:1051	the higher the α-La percentage the lower	1022:1061	α-Lactalbumin (α-La) was also associated with ethanol stability, and the higher the α-La percentage the lower were the chances of ethanol instability.
29124776	8	21	theme	lower	1119:1123	arg1	related					1174:1180	related	1174:1180	related	1174:1180	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	8	21	theme	lower	1119:1123	arg1	values					1125:1130	The lower values	1115:1130	The lower values of α-La in unstable milk samples	1115:1163	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	9	22	theme	first	1339:1343	arg1	report					1351:1356	the first field report	1335:1356	the first field report linking ethanol stability indirectly with α-La	1335:1403	This is the first field report linking ethanol stability indirectly with α-La.
29124776	9	22	theme	first	1339:1343	arg1	This					1327:1330	This	1327:1330	This	1327:1330	This is the first field report linking ethanol stability indirectly with α-La.
29124776	4	23	theme	proteins	708:715	arg1	fractions					717:725	the proteins fractions	704:725	the proteins fractions quantified by electrophoresis	704:755	An additional model was used to assess the ethanol stability status as a response to the proteins fractions quantified by electrophoresis.
29124776	1	24	theme	microbiological	178:192	arg1	acidification					194:206	microbiological acidification	178:206	microbiological acidification	178:206	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	0	25	theme	Field	0:4	arg1	findings					6:13	Field findings	0:13	Field findings about milk ethanol stability: a first report of interrelationship between α-lactalbumin and lactose.	0:114	Field findings about milk ethanol stability: a first report of interrelationship between α-lactalbumin and lactose.
29124776	8	26	theme	osmotic	1271:1277	arg1	balance					1279:1285	the osmotic balance	1267:1285	the osmotic balance of milk	1267:1293	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	0	27	theme	milk	21:24	arg1	stability					34:42	milk ethanol stability	21:42	milk ethanol stability	21:42	Field findings about milk ethanol stability: a first report of interrelationship between α-lactalbumin and lactose.
29124776	1	28	theme	soluble	279:285	arg1	salts					287:291	soluble salts	279:291	soluble salts	279:291	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	1	28	theme	soluble	279:285	arg1	activity					313:320	calcium ion activity	301:320	calcium ion activity	301:320	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	8	29	theme	α-La	1135:1138	arg1	related					1174:1180	related	1174:1180	related	1174:1180	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	8	29	theme	α-La	1135:1138	arg1	values					1125:1130	The lower values	1115:1130	The lower values of α-La in unstable milk samples	1115:1163	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	5	30	theme	total	773:777	arg1	samples					779:785	the total samples	769:785	the total samples	769:785	RESULTS Of the total samples, 68% were classified as stable and 32% as unstable to alcohol.
29124776	8	31	theme	ethanol	1308:1314	arg1	stability					1316:1324	its ethanol stability	1304:1324	its ethanol stability	1304:1324	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	1	32	theme	salts	287:291	arg1	balance					268:274	the balance	264:274	the balance of soluble salts, mainly calcium ion activity	264:320	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	5	33	dep	RESULTS	758:764	arg1	classified					797:806	classified	797:806	were classified as stable and 32% as unstable to alcohol	792:847	RESULTS Of the total samples, 68% were classified as stable and 32% as unstable to alcohol.
29124776	8	34	theme	lower	1185:1189	arg1	content					1191:1197	lower content	1185:1197	lower content of lactose	1185:1208	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	8	35	theme	unstable	1143:1150	arg1	samples					1157:1163	unstable milk samples	1143:1163	unstable milk samples	1143:1163	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	6	36	theme	content	910:916	arg1	values					892:897	low values	888:897	low values of lactose content	888:916	Milk ethanol instability increased at low values of lactose content and high values of ash percentage.
29124776	6	36	theme	content	910:916	arg1	values					927:932	high values	922:932	high values of ash percentage	922:950	Milk ethanol instability increased at low values of lactose content and high values of ash percentage.
29124776	7	37	theme	ethanol	999:1005	arg1	stability					1007:1015	ethanol stability	999:1015	ethanol stability	999:1015	α-Lactalbumin (α-La) was also associated with ethanol stability, and the higher the α-La percentage the lower were the chances of ethanol instability.
29124776	8	38	theme	lactose	1202:1208	arg1	content					1191:1197	lower content	1185:1197	lower content of lactose	1185:1208	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	2	39	theme	ethanol	376:382	arg1	stability					384:392	milk ethanol stability	371:392	milk ethanol stability	371:392	On this basis, we wanted to find out more about milk ethanol stability by studying its relationship with milk protein fractions and others major components.
29124776	0	40	theme	ethanol	26:32	arg1	stability					34:42	milk ethanol stability	21:42	milk ethanol stability	21:42	Field findings about milk ethanol stability: a first report of interrelationship between α-lactalbumin and lactose.
29124776	6	41	theme	lactose	902:908	arg1	content					910:916	lactose content	902:916	lactose content	902:916	Milk ethanol instability increased at low values of lactose content and high values of ash percentage.
29124776	7	42	theme	ethanol	1083:1089	arg1	instability					1091:1101	ethanol instability	1083:1101	ethanol instability	1083:1101	α-Lactalbumin (α-La) was also associated with ethanol stability, and the higher the α-La percentage the lower were the chances of ethanol instability.
29124776	7	43	theme	instability	1091:1101	arg1	chances					1072:1078	the chances	1068:1078	the chances of ethanol instability	1068:1101	α-Lactalbumin (α-La) was also associated with ethanol stability, and the higher the α-La percentage the lower were the chances of ethanol instability.
29124776	7	43	theme	instability	1091:1101	arg1	higher					1026:1031	higher	1026:1031	higher	1026:1031	α-Lactalbumin (α-La) was also associated with ethanol stability, and the higher the α-La percentage the lower were the chances of ethanol instability.
29124776	2	44	theme	milk	371:374	arg1	stability					384:392	milk ethanol stability	371:392	milk ethanol stability	371:392	On this basis, we wanted to find out more about milk ethanol stability by studying its relationship with milk protein fractions and others major components.
29124776	2	45	theme	protein	433:439	arg1	fractions					441:449	milk protein fractions	428:449	milk protein fractions	428:449	On this basis, we wanted to find out more about milk ethanol stability by studying its relationship with milk protein fractions and others major components.
29124776	1	46	theme	calcium	301:307	arg1	salts					287:291	soluble salts	279:291	soluble salts	279:291	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	1	46	theme	calcium	301:307	arg1	activity					313:320	calcium ion activity	301:320	calcium ion activity	301:320	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	10	47	theme	Chemical	1424:1431	arg1	Industry					1433:1440	Chemical Industry	1424:1440	Chemical Industry	1424:1440	© 2017 Society of Chemical Industry.
29124776	6	48	theme	high	922:925	arg1	values					927:932	high values	922:932	high values of ash percentage	922:950	Milk ethanol instability increased at low values of lactose content and high values of ash percentage.
29124776	3	49	theme	predictive	558:567	arg1	model					569:573	a predictive model	556:573	a predictive model comprising 180 individual raw milk samples	556:616	The influence of milk composition on ethanol stability was assessed through a predictive model comprising 180 individual raw milk samples.
29124776	2	50	theme	milk	428:431	arg1	fractions					441:449	milk protein fractions	428:449	milk protein fractions	428:449	On this basis, we wanted to find out more about milk ethanol stability by studying its relationship with milk protein fractions and others major components.
29124776	1	51	theme	ion	309:311	arg1	salts					287:291	soluble salts	279:291	soluble salts	279:291	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	1	51	theme	ion	309:311	arg1	activity					313:320	calcium ion activity	301:320	calcium ion activity	301:320	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	1	52	theme	BACKGROUND	116:125	arg1	stability					140:148	BACKGROUND Milk ethanol stability	116:148	BACKGROUND Milk ethanol stability	116:148	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	6	53	theme	low	888:890	arg1	values					892:897	low values	888:897	low values of lactose content	888:916	Milk ethanol instability increased at low values of lactose content and high values of ash percentage.
29124776	3	54	theme	milk	605:608	arg1	samples					610:616	180 individual raw milk samples	586:616	180 individual raw milk samples	586:616	The influence of milk composition on ethanol stability was assessed through a predictive model comprising 180 individual raw milk samples.
29124776	2	55	with	relationship	410:421	arg1	others					455:460	others	455:460	others	455:460	On this basis, we wanted to find out more about milk ethanol stability by studying its relationship with milk protein fractions and others major components.
29124776	2	55	with	relationship	410:421	arg1	fractions					441:449	milk protein fractions	428:449	milk protein fractions	428:449	On this basis, we wanted to find out more about milk ethanol stability by studying its relationship with milk protein fractions and others major components.
29124776	1	56	theme	Milk	127:130	arg1	stability					140:148	BACKGROUND Milk ethanol stability	116:148	BACKGROUND Milk ethanol stability	116:148	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	3	57	theme	individual	590:599	arg1	samples					610:616	180 individual raw milk samples	586:616	180 individual raw milk samples	586:616	The influence of milk composition on ethanol stability was assessed through a predictive model comprising 180 individual raw milk samples.
29124776	4	58	used	used	643:646	arg2	model					633:637	An additional model	619:637	An additional model	619:637	An additional model was used to assess the ethanol stability status as a response to the proteins fractions quantified by electrophoresis.
29124776	2	59	dep	find	351:354	arg1	out					356:358	out	356:358	out	356:358	On this basis, we wanted to find out more about milk ethanol stability by studying its relationship with milk protein fractions and others major components.
29124776	1	60	theme	ethanol	132:138	arg1	stability					140:148	BACKGROUND Milk ethanol stability	116:148	BACKGROUND Milk ethanol stability	116:148	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	4	61	theme	additional	622:631	arg1	model					633:637	An additional model	619:637	An additional model	619:637	An additional model was used to assess the ethanol stability status as a response to the proteins fractions quantified by electrophoresis.
29124776	0	62	dep	findings	6:13	arg1	report					53:58	a first report	45:58	Field findings about milk ethanol stability: a first report of interrelationship between α-lactalbumin and lactose.	0:114	Field findings about milk ethanol stability: a first report of interrelationship between α-lactalbumin and lactose.
29124776	4	63	theme	ethanol	662:668	arg1	status					680:685	the ethanol stability status	658:685	the ethanol stability status	658:685	An additional model was used to assess the ethanol stability status as a response to the proteins fractions quantified by electrophoresis.
29124776	4	63	theme	ethanol	662:668	arg1	response					692:699	a response	690:699	a response to the proteins fractions quantified by electrophoresis	690:755	An additional model was used to assess the ethanol stability status as a response to the proteins fractions quantified by electrophoresis.
29124776	8	64	theme	milk	1290:1293	arg1	balance					1279:1285	the osmotic balance	1267:1285	the osmotic balance of milk	1267:1293	CONCLUSION The lower values of α-La in unstable milk samples might be related to lower content of lactose, as α-La promotes lactose synthesis, a key component for the osmotic balance of milk and thus its ethanol stability.
29124776	1	65	theme	many	234:237	arg1	variables					239:247	many variables	234:247	many variables that influence the balance of soluble salts, mainly calcium ion activity	234:320	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	0	66	theme	first	47:51	arg1	report					53:58	a first report	45:58	Field findings about milk ethanol stability: a first report of interrelationship between α-lactalbumin and lactose.	0:114	Field findings about milk ethanol stability: a first report of interrelationship between α-lactalbumin and lactose.
29124776	3	67	theme	raw	601:603	arg1	samples					610:616	180 individual raw milk samples	586:616	180 individual raw milk samples	586:616	The influence of milk composition on ethanol stability was assessed through a predictive model comprising 180 individual raw milk samples.
29124776	1	68	with	phenomenon	218:227	arg1	variables					239:247	many variables	234:247	many variables that influence the balance of soluble salts, mainly calcium ion activity	234:320	BACKGROUND Milk ethanol stability is not only associated with microbiological acidification, but is a phenomenon with many variables that influence the balance of soluble salts, mainly calcium ion activity.
29124776	3	69	theme	milk	497:500	arg1	composition					502:512	milk composition	497:512	milk composition	497:512	The influence of milk composition on ethanol stability was assessed through a predictive model comprising 180 individual raw milk samples.
29143522	4	0	theme	faster	1120:1125	arg1	diffusion					1140:1148	faster nanoparticle diffusion	1120:1148	faster nanoparticle diffusion	1120:1148	This rapid size-shrinking characteristic not only promoted nanoparticle extravasation and accumulation in tumors benefited from the enhanced permeability and retention effect but also achieved faster nanoparticle diffusion and penetration.
29143522	1	1	theme	limited	142:148	arg1	penetration					150:160	the limited penetration	138:160	the limited penetration of nanoparticles	138:177	Currently, the limited penetration of nanoparticles remains a major challenge for antitumor nanomedicine to penetrate into the tumor tissues.
29143522	2	2	dep	metalloproteinase-2	601:619	arg1	peptide					639:645	-cleavable peptide	628:645	a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG)	592:654	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	2	2	dep	metalloproteinase-2	601:619	arg1	PLGLAG					648:653	PLGLAG	648:653	PLGLAG	648:653	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	3	3	theme	MMP-2	766:770	arg1	presence					754:761	the presence	750:761	the presence of MMP-2	750:770	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	2	4	theme	terminal	488:495	arg1	glucose					497:503	the terminal glucose	484:503	the terminal glucose of hyaluronic acid (HA)	484:527	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	7	5	theme	Meanwhile	1615:1623	arg1	nanoparticles					1646:1658	Meanwhile, macromolecule-based nanoparticles	1615:1658	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics	1615:1693	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	5	6	theme	monolayer	1415:1423	arg1	cells					1425:1429	monolayer cells	1415:1429	monolayer cells	1415:1429	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	2	7	theme	polysaccharide-modified	338:360	arg1	dendrimer					362:370	a polysaccharide-modified dendrimer	336:370	a polysaccharide-modified dendrimer	336:370	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	1	8	theme	tumor	254:258	arg1	tissues					260:266	the tumor tissues	250:266	the tumor tissues	250:266	Currently, the limited penetration of nanoparticles remains a major challenge for antitumor nanomedicine to penetrate into the tumor tissues.
29143522	0	9	theme	Drug	92:95	arg1	Penetration					97:107	Drug Penetration	92:107	Drug Penetration into Solid Tumor	92:124	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction To Enhance Drug Penetration into Solid Tumor.
29143522	6	10	theme	therapeutic	1593:1603	arg1	efficacy					1605:1612	therapeutic efficacy	1593:1612	therapeutic efficacy	1593:1612	The results confirmed that the enzyme-responsive size shrink is an implementable strategy to enhance drug penetration and to improve therapeutic efficacy.
29143522	5	11	theme	macromolecules	1232:1245	arg1	studies					1205:1211	comparative studies	1193:1211	comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids	1193:1457	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	7	12	theme	great	1793:1797	arg1	potential					1799:1807	great potential	1793:1807	great potential	1793:1807	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	7	12	theme	great	1793:1797	arg1	systems					1826:1832	nano-delivery systems	1812:1832	nano-delivery systems	1812:1832	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	3	13	theme	fast	805:808	arg1	change					815:820	a dramatic and fast size change	790:820	a dramatic and fast size change	790:820	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	7	14	dep	Meanwhile	1615:1623	arg1	macromolecule-based					1626:1644	macromolecule-based	1626:1644	macromolecule-based	1626:1644	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	4	15	theme	nanoparticle	1127:1138	arg1	diffusion					1140:1148	faster nanoparticle diffusion	1120:1148	faster nanoparticle diffusion	1120:1148	This rapid size-shrinking characteristic not only promoted nanoparticle extravasation and accumulation in tumors benefited from the enhanced permeability and retention effect but also achieved faster nanoparticle diffusion and penetration.
29143522	7	16	theme	nano-delivery	1812:1824	arg1	potential					1799:1807	great potential	1793:1807	great potential	1793:1807	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	7	16	theme	nano-delivery	1812:1824	arg1	systems					1826:1832	nano-delivery systems	1812:1832	nano-delivery systems	1812:1832	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	4	17	theme	enhanced	1059:1066	arg1	effect					1095:1100	the enhanced permeability and retention effect	1055:1100	the enhanced permeability and retention effect	1055:1100	This rapid size-shrinking characteristic not only promoted nanoparticle extravasation and accumulation in tumors benefited from the enhanced permeability and retention effect but also achieved faster nanoparticle diffusion and penetration.
29143522	5	18	theme	particle	1337:1344	arg1	size					1346:1349	a similar particle size	1327:1349	a similar particle size	1327:1349	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	2	19	theme	matrix	594:599	arg1	MMP-2					622:626	MMP-2	622:626	MMP-2	622:626	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	2	19	theme	matrix	594:599	arg1	metalloproteinase-2					601:619	a matrix metalloproteinase-2	592:619	a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG)	592:654	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	3	20	theme	PLGLAG	919:924	arg1	cleavage					907:914	cleavage	907:914	cleavage of PLGLAG	907:924	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	4	21	theme	nanoparticle	986:997	arg1	extravasation					999:1011	nanoparticle extravasation	986:1011	nanoparticle extravasation	986:1011	This rapid size-shrinking characteristic not only promoted nanoparticle extravasation and accumulation in tumors benefited from the enhanced permeability and retention effect but also achieved faster nanoparticle diffusion and penetration.
29143522	4	22	theme	permeability	1068:1079	arg1	effect					1095:1100	the enhanced permeability and retention effect	1055:1100	the enhanced permeability and retention effect	1055:1100	This rapid size-shrinking characteristic not only promoted nanoparticle extravasation and accumulation in tumors benefited from the enhanced permeability and retention effect but also achieved faster nanoparticle diffusion and penetration.
29143522	2	23	theme	acid	519:522	arg1	glucose					497:503	the terminal glucose	484:503	the terminal glucose of hyaluronic acid (HA)	484:527	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	6	24	dep	strategy	1541:1548	arg1	improve					1585:1591	improve	1585:1591	to improve therapeutic efficacy	1582:1612	The results confirmed that the enzyme-responsive size shrink is an implementable strategy to enhance drug penetration and to improve therapeutic efficacy.
29143522	6	24	dep	strategy	1541:1548	arg1	enhance					1553:1559	enhance	1553:1559	to enhance drug penetration	1550:1576	The results confirmed that the enzyme-responsive size shrink is an implementable strategy to enhance drug penetration and to improve therapeutic efficacy.
29143522	4	25	theme	rapid	932:936	arg1	characteristic					953:966	This rapid size-shrinking characteristic	927:966	This rapid size-shrinking characteristic	927:966	This rapid size-shrinking characteristic not only promoted nanoparticle extravasation and accumulation in tumors benefited from the enhanced permeability and retention effect but also achieved faster nanoparticle diffusion and penetration.
29143522	5	26	theme	similar	1329:1335	arg1	size					1346:1349	a similar particle size	1327:1349	a similar particle size	1327:1349	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	1	27	theme	nanoparticles	165:177	arg1	penetration					150:160	the limited penetration	138:160	the limited penetration of nanoparticles	138:177	Currently, the limited penetration of nanoparticles remains a major challenge for antitumor nanomedicine to penetrate into the tumor tissues.
29143522	0	28	theme	HA	16:17	arg1	Dendrimers					51:60	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers	0:60	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction	0:79	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction To Enhance Drug Penetration into Solid Tumor.
29143522	7	29	theme	size-variable	1665:1677	arg1	characteristics					1679:1693	size-variable characteristics	1665:1693	size-variable characteristics	1665:1693	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	2	30	theme	hyaluronic	508:517	arg1	HA					525:526	HA	525:526	HA	525:526	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	2	30	theme	hyaluronic	508:517	arg1	acid					519:522	hyaluronic acid	508:522	hyaluronic acid (HA)	508:527	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	2	31	theme	delivery	311:318	arg1	system					320:325	a size-shrinkable drug delivery system	288:325	a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction	288:673	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	4	32	theme	retention	1085:1093	arg1	effect					1095:1100	the enhanced permeability and retention effect	1055:1100	the enhanced permeability and retention effect	1055:1100	This rapid size-shrinking characteristic not only promoted nanoparticle extravasation and accumulation in tumors benefited from the enhanced permeability and retention effect but also achieved faster nanoparticle diffusion and penetration.
29143522	6	33	theme	implementable	1527:1539	arg1	strategy					1541:1548	an implementable strategy	1524:1548	an implementable strategy to enhance drug penetration and to improve therapeutic efficacy	1524:1612	The results confirmed that the enzyme-responsive size shrink is an implementable strategy to enhance drug penetration and to improve therapeutic efficacy.
29143522	6	33	theme	implementable	1527:1539	arg1	shrink					1514:1519	the enzyme-responsive size shrink	1487:1519	the enzyme-responsive size shrink	1487:1519	The results confirmed that the enzyme-responsive size shrink is an implementable strategy to enhance drug penetration and to improve therapeutic efficacy.
29143522	0	34	theme	MMP-2-Sensitive	0:14	arg1	Dendrimers					51:60	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers	0:60	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction	0:79	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction To Enhance Drug Penetration into Solid Tumor.
29143522	7	35	theme	delivery	1764:1771	arg1	they					1734:1737	they	1734:1737	they	1734:1737	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	7	35	theme	delivery	1764:1771	arg1	systems					1773:1779	gene delivery systems	1759:1779	gene delivery systems	1759:1779	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	2	36	theme	drug	306:309	arg1	system					320:325	a size-shrinkable drug delivery system	288:325	a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction	288:673	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	7	37	theme	drug	1712:1715	arg1	penetration					1717:1727	drug penetration	1712:1727	drug penetration	1712:1727	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	2	38	theme	microenvironment	383:398	arg1	responsiveness					400:413	tumor microenvironment responsiveness	377:413	tumor microenvironment responsiveness for the first time to our knowledge	377:449	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	0	39	theme	Poly	34:37	arg1	Dendrimers					51:60	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers	0:60	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction	0:79	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction To Enhance Drug Penetration into Solid Tumor.
29143522	2	40	theme	-cleavable	628:637	arg1	peptide					639:645	-cleavable peptide	628:645	a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG)	592:654	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	2	40	theme	-cleavable	628:637	arg1	PLGLAG					648:653	PLGLAG	648:653	PLGLAG	648:653	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	2	41	theme	size-shrinkable	290:304	arg1	system					320:325	a size-shrinkable drug delivery system	288:325	a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction	288:673	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	1	42	theme	major	189:193	arg1	challenge					195:203	a major challenge	187:203	a major challenge for antitumor nanomedicine to penetrate into the tumor tissues	187:266	Currently, the limited penetration of nanoparticles remains a major challenge for antitumor nanomedicine to penetrate into the tumor tissues.
29143522	0	43	theme	End-Conjugated	19:32	arg1	Dendrimers					51:60	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers	0:60	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction	0:79	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction To Enhance Drug Penetration into Solid Tumor.
29143522	0	44	theme	Solid	114:118	arg1	Tumor					120:124	Solid Tumor	114:124	Solid Tumor	114:124	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction To Enhance Drug Penetration into Solid Tumor.
29143522	6	45	theme	size	1509:1512	arg1	strategy					1541:1548	an implementable strategy	1524:1548	an implementable strategy to enhance drug penetration and to improve therapeutic efficacy	1524:1612	The results confirmed that the enzyme-responsive size shrink is an implementable strategy to enhance drug penetration and to improve therapeutic efficacy.
29143522	6	45	theme	size	1509:1512	arg1	shrink					1514:1519	the enzyme-responsive size shrink	1487:1519	the enzyme-responsive size shrink	1487:1519	The results confirmed that the enzyme-responsive size shrink is an implementable strategy to enhance drug penetration and to improve therapeutic efficacy.
29143522	3	46	theme	dendrimer	849:857	arg1	blocks					868:873	their dendrimer building blocks	843:873	their dendrimer building blocks (∼10 nm in diameter)	843:894	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	3	47	from	nm	880:881	arg1	diameter					886:893	diameter	886:893	diameter	886:893	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	0	48	theme	amidoamine	39:48	arg1	Dendrimers					51:60	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers	0:60	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction	0:79	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction To Enhance Drug Penetration into Solid Tumor.
29143522	3	49	theme	initial	703:709	arg1	size					711:714	an initial size	700:714	an initial size of ∼200 nm	700:725	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	3	50	theme	building	859:866	arg1	blocks					868:873	their dendrimer building blocks	843:873	their dendrimer building blocks (∼10 nm in diameter)	843:894	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	2	51	theme	superficial	536:546	arg1	amidogen					548:555	the superficial amidogen	532:555	the superficial amidogen of poly(amidoamine) (PAMAM)	532:583	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	5	52	theme	multicellular	1435:1447	arg1	spheroids					1449:1457	multicellular spheroids	1435:1457	multicellular spheroids	1435:1457	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	5	53	dep	macromolecules	1232:1245	arg1	synthesized					1310:1320	synthesized	1310:1320	synthesized with a similar particle size, surface charge, and chemical composition	1310:1391	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	5	53	dep	macromolecules	1232:1245	arg1	evaluated					1397:1405	evaluated	1397:1405	evaluated in both monolayer cells and multicellular spheroids	1397:1457	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	1	54	theme	antitumor	209:217	arg1	nanomedicine					219:230	antitumor nanomedicine	209:230	antitumor nanomedicine to penetrate into the tumor tissues	209:266	Currently, the limited penetration of nanoparticles remains a major challenge for antitumor nanomedicine to penetrate into the tumor tissues.
29143522	7	55	with	nanoparticles	1646:1658	arg1	characteristics					1679:1693	size-variable characteristics	1665:1693	size-variable characteristics	1665:1693	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	5	56	theme	surface	1352:1358	arg1	charge					1360:1365	surface charge	1352:1365	surface charge	1352:1365	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	2	57	theme	click	660:664	arg1	reaction					666:673	click reaction	660:673	click reaction	660:673	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	7	58	used	used	1751:1754	arg2	they					1734:1737	they	1734:1737	they	1734:1737	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	7	58	used	used	1751:1754	arg2	systems					1773:1779	gene delivery systems	1759:1779	gene delivery systems	1759:1779	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	2	59	with	dendrimer	362:370	arg1	responsiveness					400:413	tumor microenvironment responsiveness	377:413	tumor microenvironment responsiveness for the first time to our knowledge	377:449	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	5	60	theme	chemical	1372:1379	arg1	composition					1381:1391	chemical composition	1372:1391	chemical composition	1372:1391	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	6	61	theme	enzyme-responsive	1491:1507	arg1	strategy					1541:1548	an implementable strategy	1524:1548	an implementable strategy to enhance drug penetration and to improve therapeutic efficacy	1524:1612	The results confirmed that the enzyme-responsive size shrink is an implementable strategy to enhance drug penetration and to improve therapeutic efficacy.
29143522	6	61	theme	enzyme-responsive	1491:1507	arg1	shrink					1514:1519	the enzyme-responsive size shrink	1487:1519	the enzyme-responsive size shrink	1487:1519	The results confirmed that the enzyme-responsive size shrink is an implementable strategy to enhance drug penetration and to improve therapeutic efficacy.
29143522	3	62	theme	nm	724:725	arg1	size					711:714	an initial size	700:714	an initial size of ∼200 nm	700:725	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	2	63	theme	poly	560:563	arg1	amidogen					548:555	the superficial amidogen	532:555	the superficial amidogen of poly(amidoamine) (PAMAM)	532:583	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	3	64	contain	had	696:698	arg1	nanoparticles					682:694	These nanoparticles	676:694	These nanoparticles	676:694	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	3	64	contain	had	696:698	arg2	size					711:714	an initial size	700:714	an initial size of ∼200 nm	700:725	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	3	65	theme	dramatic	792:799	arg1	change					815:820	a dramatic and fast size change	790:820	a dramatic and fast size change	790:820	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	6	66	theme	drug	1561:1564	arg1	penetration					1566:1576	drug penetration	1561:1576	drug penetration	1561:1576	The results confirmed that the enzyme-responsive size shrink is an implementable strategy to enhance drug penetration and to improve therapeutic efficacy.
29143522	7	67	theme	gene	1759:1762	arg1	they					1734:1737	they	1734:1737	they	1734:1737	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	7	67	theme	gene	1759:1762	arg1	systems					1773:1779	gene delivery systems	1759:1779	gene delivery systems	1759:1779	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	7	68	dep	promote	1704:1710	arg1	only					1699:1702	only	1699:1702	only	1699:1702	Meanwhile, macromolecule-based nanoparticles with size-variable characteristics not only promote drug penetration, but they can also be used as gene delivery systems, suggesting great potential as nano-delivery systems.
29143522	5	69	theme	MMP-2-insensitive	1266:1282	arg1	HA-PAMAM					1300:1307	HA-PAMAM	1300:1307	HA-PAMAM	1300:1307	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	5	69	theme	MMP-2-insensitive	1266:1282	arg1	macromolecules					1284:1297	MMP-2-insensitive macromolecules	1266:1297	MMP-2-insensitive macromolecules (HA-PAMAM)	1266:1308	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	5	70	theme	comparative	1193:1203	arg1	studies					1205:1211	comparative studies	1193:1211	comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids	1193:1457	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	0	71	theme	Click	66:70	arg1	Reaction					72:79	Click Reaction	66:79	Click Reaction	66:79	MMP-2-Sensitive HA End-Conjugated Poly(amidoamine) Dendrimers via Click Reaction To Enhance Drug Penetration into Solid Tumor.
29143522	2	72	theme	first	423:427	arg1	time					429:432	the first time	419:432	the first time	419:432	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	5	73	theme	MMP-2-sensitive	1216:1230	arg1	HA-pep-PAMAM					1248:1259	HA-pep-PAMAM	1248:1259	HA-pep-PAMAM	1248:1259	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	5	73	theme	MMP-2-sensitive	1216:1230	arg1	macromolecules					1232:1245	MMP-2-sensitive macromolecules	1216:1245	MMP-2-sensitive macromolecules (HA-pep-PAMAM)	1216:1260	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	2	74	theme	tumor	377:381	arg1	responsiveness					400:413	tumor microenvironment responsiveness	377:413	tumor microenvironment responsiveness for the first time to our knowledge	377:449	Herein, we propose a size-shrinkable drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction.
29143522	5	75	theme	macromolecules	1284:1297	arg1	studies					1205:1211	comparative studies	1193:1211	comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids	1193:1457	We have further conducted comparative studies of MMP-2-sensitive macromolecules (HA-pep-PAMAM) and MMP-2-insensitive macromolecules (HA-PAMAM) synthesized with a similar particle size, surface charge, and chemical composition and evaluated in both monolayer cells and multicellular spheroids.
29143522	3	76	dep	blocks	868:873	arg1	nm					880:881	∼10 nm	876:881	∼10 nm in diameter	876:893	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
29143522	4	77	theme	size-shrinking	938:951	arg1	characteristic					953:966	This rapid size-shrinking characteristic	927:966	This rapid size-shrinking characteristic	927:966	This rapid size-shrinking characteristic not only promoted nanoparticle extravasation and accumulation in tumors benefited from the enhanced permeability and retention effect but also achieved faster nanoparticle diffusion and penetration.
29143522	3	78	theme	size	810:813	arg1	change					815:820	a dramatic and fast size change	790:820	a dramatic and fast size change	790:820	These nanoparticles had an initial size of ∼200 nm, but once deposited in the presence of MMP-2, they experienced a dramatic and fast size change and dissociated into their dendrimer building blocks (∼10 nm in diameter) because of cleavage of PLGLAG.
25694165	5	0	theme	Mg	650:651	arg1	amounts					639:645	various amounts	631:645	various amounts of Mg(2+)	631:655	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	5	0	theme	Mg	650:651	arg1	2+					653:654	2+	653:654	2+	653:654	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	5	0	theme	Mg	650:651	arg1	Mg					650:651	Mg	650:651	Mg(2+)	650:655	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	6	1	theme	elastic	868:874	arg1	modulus					876:882	elastic modulus	868:882	elastic modulus	868:882	The results showed that the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus, could be well tuned by controlling the amount of Mg(2+) incorporated.
25694165	0	2	theme	adhesion	87:94	arg1	ability					96:102	cell adhesion ability	82:102	cell adhesion ability	82:102	Incorporation of magnesium ions into photo-crosslinked alginate hydrogel enhanced cell adhesion ability.
25694165	7	3	theme	osteoblasts	1047:1057	arg1	spread					1037:1042	spread	1037:1042	spread	1037:1042	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	7	3	theme	osteoblasts	1047:1057	arg1	attachment					1022:1031	attachment	1022:1031	attachment	1022:1031	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	5	4	theme	basic	611:615	arg1	hydrogel					584:591	Photo-crosslinked alginate hydrogel	557:591	Photo-crosslinked alginate hydrogel	557:591	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	5	4	theme	basic	611:615	arg1	backbone					617:624	the basic backbone	607:624	the basic backbone	607:624	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	6	5	theme	surface	804:810	arg1	structure					812:820	surface structure	804:820	surface structure	804:820	The results showed that the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus, could be well tuned by controlling the amount of Mg(2+) incorporated.
25694165	7	6	from	attachment	1022:1031	arg1	hydrogels					1066:1074	the hydrogels	1062:1074	the hydrogels	1062:1074	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	8	7	theme	tissue	1191:1196	arg1	engineering					1198:1208	bone tissue engineering	1186:1208	bone tissue engineering	1186:1208	These characteristics make Mg(2+) -incorporated photo-crosslinked alginate hydrogel a promising scaffold for bone tissue engineering.
25694165	0	8	theme	alginate	55:62	arg1	hydrogel					64:71	photo-crosslinked alginate hydrogel	37:71	photo-crosslinked alginate hydrogel	37:71	Incorporation of magnesium ions into photo-crosslinked alginate hydrogel enhanced cell adhesion ability.
25694165	4	9	theme	durable	483:489	arg1	hydrogel					514:521	a durable, crosslinked, alginate hydrogel	481:521	a durable, crosslinked, alginate hydrogel with a dual-crosslinking network	481:554	In this study, for the first time, we fabricated a durable, crosslinked, alginate hydrogel with a dual-crosslinking network.
25694165	5	10	theme	various	631:637	arg1	amounts					639:645	various amounts	631:645	various amounts of Mg(2+)	631:655	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	5	10	theme	various	631:637	arg1	2+					653:654	2+	653:654	2+	653:654	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	5	10	theme	various	631:637	arg1	Mg					650:651	Mg	650:651	Mg(2+)	650:655	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	1	11	from	interest	155:162	arg1	engineering					174:184	tissue engineering	167:184	tissue engineering	167:184	Photo-crosslinked alginate hydrogel attracts wide interest in tissue engineering because of its excellent controllability and stability.
25694165	1	12	theme	excellent	201:209	arg1	controllability					211:225	its excellent controllability	197:225	its excellent controllability	197:225	Photo-crosslinked alginate hydrogel attracts wide interest in tissue engineering because of its excellent controllability and stability.
25694165	8	13	theme	photo-crosslinked	1125:1141	arg1	hydrogel					1152:1159	Mg(2+) -incorporated photo-crosslinked alginate hydrogel	1104:1159	Mg(2+) -incorporated photo-crosslinked alginate hydrogel	1104:1159	These characteristics make Mg(2+) -incorporated photo-crosslinked alginate hydrogel a promising scaffold for bone tissue engineering.
25694165	1	14	link	Photo-crosslinked	105:121	arg1	hydrogel					132:139	Photo-crosslinked alginate hydrogel	105:139	Photo-crosslinked alginate hydrogel	105:139	Photo-crosslinked alginate hydrogel attracts wide interest in tissue engineering because of its excellent controllability and stability.
25694165	6	15	theme	hydrogels	783:791	arg1	properties					765:774	the physicochemical properties	745:774	the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus,	745:883	The results showed that the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus, could be well tuned by controlling the amount of Mg(2+) incorporated.
25694165	8	16	theme	-incorporated	1111:1123	arg1	hydrogel					1152:1159	Mg(2+) -incorporated photo-crosslinked alginate hydrogel	1104:1159	Mg(2+) -incorporated photo-crosslinked alginate hydrogel	1104:1159	These characteristics make Mg(2+) -incorporated photo-crosslinked alginate hydrogel a promising scaffold for bone tissue engineering.
25694165	4	17	dep	durable	483:489	arg1	crosslinked					492:502	crosslinked	492:502	crosslinked	492:502	In this study, for the first time, we fabricated a durable, crosslinked, alginate hydrogel with a dual-crosslinking network.
25694165	4	17	dep	durable	483:489	arg1	alginate					505:512	alginate	505:512	alginate	505:512	In this study, for the first time, we fabricated a durable, crosslinked, alginate hydrogel with a dual-crosslinking network.
25694165	8	18	theme	promising	1163:1171	arg1	scaffold					1173:1180	a promising scaffold	1161:1180	a promising scaffold for bone tissue engineering	1161:1208	These characteristics make Mg(2+) -incorporated photo-crosslinked alginate hydrogel a promising scaffold for bone tissue engineering.
25694165	0	19	link	photo-crosslinked	37:53	arg1	hydrogel					64:71	photo-crosslinked alginate hydrogel	37:71	photo-crosslinked alginate hydrogel	37:71	Incorporation of magnesium ions into photo-crosslinked alginate hydrogel enhanced cell adhesion ability.
25694165	1	20	theme	Photo-crosslinked	105:121	arg1	hydrogel					132:139	Photo-crosslinked alginate hydrogel	105:139	Photo-crosslinked alginate hydrogel	105:139	Photo-crosslinked alginate hydrogel attracts wide interest in tissue engineering because of its excellent controllability and stability.
25694165	0	21	theme	ions	27:30	arg1	Incorporation					0:12	Incorporation	0:12	Incorporation of magnesium ions into photo-crosslinked alginate hydrogel	0:71	Incorporation of magnesium ions into photo-crosslinked alginate hydrogel enhanced cell adhesion ability.
25694165	3	22	dep	ions	362:365	arg1	Mg					368:369	Mg	368:369	Mg(2+)	368:373	Plenty of studies have confirmed that magnesium ions (Mg(2+) ) can efficiently improve the attachment of osteoblasts.
25694165	3	22	dep	ions	362:365	arg1	2+					371:372	2+	371:372	2+	371:372	Plenty of studies have confirmed that magnesium ions (Mg(2+) ) can efficiently improve the attachment of osteoblasts.
25694165	7	23	dep	attachment	1022:1031	arg1	the					1018:1020	the	1018:1020	the	1018:1020	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	1	24	theme	alginate	123:130	arg1	hydrogel					132:139	Photo-crosslinked alginate hydrogel	105:139	Photo-crosslinked alginate hydrogel	105:139	Photo-crosslinked alginate hydrogel attracts wide interest in tissue engineering because of its excellent controllability and stability.
25694165	0	25	theme	magnesium	17:25	arg1	ions					27:30	magnesium ions	17:30	magnesium ions	17:30	Incorporation of magnesium ions into photo-crosslinked alginate hydrogel enhanced cell adhesion ability.
25694165	7	26	from	spread	1037:1042	arg1	hydrogels					1066:1074	the hydrogels	1062:1074	the hydrogels	1062:1074	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	3	27	theme	studies	324:330	arg1	Plenty					314:319	Plenty	314:319	Plenty of studies	314:330	Plenty of studies have confirmed that magnesium ions (Mg(2+) ) can efficiently improve the attachment of osteoblasts.
25694165	4	28	theme	first	455:459	arg1	time					461:464	the first time	451:464	the first time	451:464	In this study, for the first time, we fabricated a durable, crosslinked, alginate hydrogel with a dual-crosslinking network.
25694165	4	29	with	hydrogel	514:521	arg1	network					548:554	a dual-crosslinking network	528:554	a dual-crosslinking network	528:554	In this study, for the first time, we fabricated a durable, crosslinked, alginate hydrogel with a dual-crosslinking network.
25694165	0	30	theme	photo-crosslinked	37:53	arg1	hydrogel					64:71	photo-crosslinked alginate hydrogel	37:71	photo-crosslinked alginate hydrogel	37:71	Incorporation of magnesium ions into photo-crosslinked alginate hydrogel enhanced cell adhesion ability.
25694165	6	31	theme	Mg	934:935	arg1	amount					924:929	the amount	920:929	the amount of Mg(2+) incorporated	920:952	The results showed that the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus, could be well tuned by controlling the amount of Mg(2+) incorporated.
25694165	6	31	theme	Mg	934:935	arg1	2+					937:938	2+	937:938	2+	937:938	The results showed that the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus, could be well tuned by controlling the amount of Mg(2+) incorporated.
25694165	6	31	theme	Mg	934:935	arg1	Mg					934:935	Mg	934:935	Mg(2+)	934:939	The results showed that the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus, could be well tuned by controlling the amount of Mg(2+) incorporated.
25694165	5	32	theme	ionic	701:705	arg1	crosslinking					707:718	ionic crosslinking	701:718	ionic crosslinking	701:718	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	8	33	theme	bone	1186:1189	arg1	engineering					1198:1208	bone tissue engineering	1186:1208	bone tissue engineering	1186:1208	These characteristics make Mg(2+) -incorporated photo-crosslinked alginate hydrogel a promising scaffold for bone tissue engineering.
25694165	5	34	link	Photo-crosslinked	557:573	arg1	hydrogel					584:591	Photo-crosslinked alginate hydrogel	557:591	Photo-crosslinked alginate hydrogel	557:591	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	5	34	link	Photo-crosslinked	557:573	arg1	backbone					617:624	the basic backbone	607:624	the basic backbone	607:624	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	6	35	theme	ion	852:854	arg1	release					856:862	ion release	852:862	ion release	852:862	The results showed that the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus, could be well tuned by controlling the amount of Mg(2+) incorporated.
25694165	2	36	theme	hydrophilic	262:272	arg1	property					274:281	its highly hydrophilic property	251:281	its highly hydrophilic property	251:281	However, its highly hydrophilic property makes cell adhesion difficult.
25694165	7	37	theme	certain	970:976	arg1	2+					991:992	2+	991:992	2+	991:992	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	7	37	theme	certain	970:976	arg1	Mg					988:989	Mg	988:989	Mg(2+)	988:993	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	7	37	theme	certain	970:976	arg1	amount					978:983	a certain amount	968:983	a certain amount of Mg(2+)	968:993	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	6	38	theme	physicochemical	749:763	arg1	properties					765:774	the physicochemical properties	745:774	the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus,	745:883	The results showed that the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus, could be well tuned by controlling the amount of Mg(2+) incorporated.
25694165	3	39	theme	osteoblasts	419:429	arg1	attachment					405:414	the attachment	401:414	the attachment of osteoblasts	401:429	Plenty of studies have confirmed that magnesium ions (Mg(2+) ) can efficiently improve the attachment of osteoblasts.
25694165	4	40	theme	dual-crosslinking	530:546	arg1	network					548:554	a dual-crosslinking network	528:554	a dual-crosslinking network	528:554	In this study, for the first time, we fabricated a durable, crosslinked, alginate hydrogel with a dual-crosslinking network.
25694165	3	41	theme	magnesium	352:360	arg1	ions					362:365	magnesium ions	352:365	magnesium ions (Mg(2+) )	352:375	Plenty of studies have confirmed that magnesium ions (Mg(2+) ) can efficiently improve the attachment of osteoblasts.
25694165	5	42	theme	Photo-crosslinked	557:573	arg1	hydrogel					584:591	Photo-crosslinked alginate hydrogel	557:591	Photo-crosslinked alginate hydrogel	557:591	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	5	42	theme	Photo-crosslinked	557:573	arg1	backbone					617:624	the basic backbone	607:624	the basic backbone	607:624	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	1	43	theme	wide	150:153	arg1	interest					155:162	wide interest	150:162	wide interest in tissue engineering	150:184	Photo-crosslinked alginate hydrogel attracts wide interest in tissue engineering because of its excellent controllability and stability.
25694165	6	44	theme	swelling	836:843	arg1	ratio					845:849	swelling ratio	836:849	swelling ratio	836:849	The results showed that the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus, could be well tuned by controlling the amount of Mg(2+) incorporated.
25694165	5	45	theme	alginate	575:582	arg1	hydrogel					584:591	Photo-crosslinked alginate hydrogel	557:591	Photo-crosslinked alginate hydrogel	557:591	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	5	45	theme	alginate	575:582	arg1	backbone					617:624	the basic backbone	607:624	the basic backbone	607:624	Photo-crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg(2+) were incorporated into the hydrogel through ionic crosslinking.
25694165	7	46	theme	Mg	988:989	arg1	2+					991:992	2+	991:992	2+	991:992	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	7	46	theme	Mg	988:989	arg1	Mg					988:989	Mg	988:989	Mg(2+)	988:993	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	7	46	theme	Mg	988:989	arg1	amount					978:983	a certain amount	968:983	a certain amount of Mg(2+)	968:993	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	0	47	theme	cell	82:85	arg1	ability					96:102	cell adhesion ability	82:102	cell adhesion ability	82:102	Incorporation of magnesium ions into photo-crosslinked alginate hydrogel enhanced cell adhesion ability.
25694165	2	48	theme	cell	289:292	arg1	adhesion					294:301	cell adhesion	289:301	cell adhesion	289:301	However, its highly hydrophilic property makes cell adhesion difficult.
25694165	1	49	theme	tissue	167:172	arg1	engineering					174:184	tissue engineering	167:184	tissue engineering	167:184	Photo-crosslinked alginate hydrogel attracts wide interest in tissue engineering because of its excellent controllability and stability.
25694165	7	50	attach	attachment	1022:1031	arg3	hydrogels					1066:1074	the hydrogels	1062:1074	the hydrogels	1062:1074	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	7	50	attach	attachment	1022:1031	arg2	osteoblasts					1047:1057	osteoblasts	1047:1057	osteoblasts	1047:1057	In addition, a certain amount of Mg(2+) significantly improved the attachment and spread of osteoblasts on the hydrogels.
25694165	8	51	link	photo-crosslinked	1125:1141	arg1	hydrogel					1152:1159	Mg(2+) -incorporated photo-crosslinked alginate hydrogel	1104:1159	Mg(2+) -incorporated photo-crosslinked alginate hydrogel	1104:1159	These characteristics make Mg(2+) -incorporated photo-crosslinked alginate hydrogel a promising scaffold for bone tissue engineering.
25694165	8	52	theme	alginate	1143:1150	arg1	hydrogel					1152:1159	Mg(2+) -incorporated photo-crosslinked alginate hydrogel	1104:1159	Mg(2+) -incorporated photo-crosslinked alginate hydrogel	1104:1159	These characteristics make Mg(2+) -incorporated photo-crosslinked alginate hydrogel a promising scaffold for bone tissue engineering.
26313619	3	0	theme	microsphere	502:512	arg1	compositions					519:530	four microsphere SPIO compositions	497:530	four microsphere SPIO compositions	497:530	The r2* relaxivity for each of four microsphere SPIO compositions was determined from 32 phantoms constructed with agarose gel and in eight concentrations from each of the four compositions.
26313619	9	1	theme	microsphere	1363:1373	arg1	concentration					1375:1387	the 2% SPIO-labeled yttrium microsphere concentration	1335:1387	the 2% SPIO-labeled yttrium microsphere concentration	1335:1387	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	12	2	theme	%	1916:1916	arg1	SPIO					1918:1921	2% SPIO	1915:1921	2% SPIO	1915:1921	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	5	3	theme	microspheres	908:919	arg1	doses					855:859	doses	855:859	doses of 5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres	855:919	For quantitative studies, doses of 5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres were infused into 24 rats (six rats per group).
26313619	7	4	theme	Pearson	1053:1059	arg1	correlation					1061:1071	Pearson correlation	1053:1071	Pearson correlation	1053:1071	Pearson correlation, analysis of variance, and intraclass correlation analyses were performed to compare MR imaging measurements in phantoms and animal models.
26313619	4	5	theme	transcatheter	670:682	arg1	procedures					693:702	Intrahepatic transcatheter infusion procedures	657:702	Intrahepatic transcatheter infusion procedures	657:702	Intrahepatic transcatheter infusion procedures were performed in rats by using each of the four compositions before MR imaging to visualize distributions within the liver.
26313619	1	6	theme	labeling	189:196	arg1	microspheres					206:217	labeling yttrium microspheres	189:217	labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	189:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	12	7	theme	microspheres	1889:1900	arg1	measurements					1865:1876	CONCLUSION MR imaging R2* measurements	1839:1876	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO	1839:1921	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	11	8	theme	delivery	1737:1744	arg1	measurements					1685:1696	R2*-based measurements	1675:1696	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery	1675:1744	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	3	9	theme	agarose	581:587	arg1	gel					589:591	agarose gel	581:591	agarose gel	581:591	The r2* relaxivity for each of four microsphere SPIO compositions was determined from 32 phantoms constructed with agarose gel and in eight concentrations from each of the four compositions.
26313619	9	10	theme	known	1415:1419	arg1	concentrations					1429:1442	known phantom concentrations	1415:1442	known phantom concentrations	1415:1442	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	8	11	theme	RESULTS	1213:1219	arg1	relaxivity					1235:1244	RESULTS Increased r2* relaxivity	1213:1244	RESULTS Increased r2* relaxivity	1213:1244	RESULTS Increased r2* relaxivity was observed with incremental increases of SPIO microsphere content.
26313619	11	12	theme	yttrium	1717:1723	arg1	delivery					1737:1744	2% SPIO-labeled yttrium microsphere delivery	1701:1744	2% SPIO-labeled yttrium microsphere delivery	1701:1744	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	10	13	theme	marked	1654:1659	arg1	voids					1668:1672	marked signal voids	1654:1672	marked signal voids	1654:1672	Microspheres were heterogeneously distributed within each liver; increasing microsphere SPIO content produced marked signal voids.
26313619	1	14	theme	increasing	224:233	arg1	material					282:289	superparamagnetic iron oxide (SPIO) material	246:289	superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	246:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	1	14	theme	increasing	224:233	arg1	amounts					235:241	increasing amounts	224:241	increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	224:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	12	15	theme	*	1863:1863	arg1	measurements					1865:1876	CONCLUSION MR imaging R2* measurements	1839:1876	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO	1839:1921	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	12	16	theme	rat	1991:1993	arg1	model					1995:1999	a rat model	1989:1999	a rat model	1989:1999	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	0	17	from	Delivery	94:101	arg1	Model					115:119	a Rodent Model	106:119	a Rodent Model	106:119	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.
26313619	11	18	theme	<	1830:1830	arg1	P					1828:1828	P < .001	1828:1835	P < .001	1828:1835	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	8	19	theme	r2	1231:1232	arg1	relaxivity					1235:1244	RESULTS Increased r2* relaxivity	1213:1244	RESULTS Increased r2* relaxivity	1213:1244	RESULTS Increased r2* relaxivity was observed with incremental increases of SPIO microsphere content.
26313619	5	20	theme	%	885:885	arg1	microspheres					908:919	5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres	864:919	5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres	864:919	For quantitative studies, doses of 5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres were infused into 24 rats (six rats per group).
26313619	0	21	from	Model	115:119	arg1	Quantification					49:62	MR Imaging Quantification	38:62	MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model	38:119	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.
26313619	11	22	theme	-based	1678:1683	arg1	measurements					1685:1696	R2*-based measurements	1675:1696	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery	1675:1744	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	9	23	theme	=	1450:1450	arg1	R					1445:1445	R(2)	1445:1448	R(2) = 1.00	1445:1455	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	6	24	theme	imaging	972:978	arg1	measurements					984:995	MR imaging R2* measurements	969:995	MR imaging R2* measurements	969:995	MR imaging R2* measurements were used to quantify the dose delivered to each liver.
26313619	3	25	theme	r2	470:471	arg1	relaxivity					474:483	The r2* relaxivity	466:483	The r2* relaxivity for each of four microsphere SPIO compositions	466:530	The r2* relaxivity for each of four microsphere SPIO compositions was determined from 32 phantoms constructed with agarose gel and in eight concentrations from each of the four compositions.
26313619	8	26	theme	incremental	1264:1274	arg1	increases					1276:1284	incremental increases	1264:1284	incremental increases of SPIO microsphere content	1264:1312	RESULTS Increased r2* relaxivity was observed with incremental increases of SPIO microsphere content.
26313619	11	27	theme	R2	1675:1676	arg1	measurements					1685:1696	R2*-based measurements	1675:1696	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery	1675:1744	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	9	28	theme	concentration	1375:1387	arg1	measurements					1319:1330	R2* measurements	1315:1330	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration	1315:1387	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	5	29	dep	microspheres	908:919	arg1	mg					881:882	mg	881:882	mg	881:882	For quantitative studies, doses of 5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres were infused into 24 rats (six rats per group).
26313619	11	30	theme	%	1702:1702	arg1	delivery					1737:1744	2% SPIO-labeled yttrium microsphere delivery	1701:1744	2% SPIO-labeled yttrium microsphere delivery	1701:1744	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	0	31	theme	Delivery	94:101	arg1	Quantification					49:62	MR Imaging Quantification	38:62	MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model	38:119	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.
26313619	1	32	from	imaging	319:325	arg1	models					349:354	phantom and rodent models	330:354	phantom and rodent models	330:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	8	33	theme	microsphere	1294:1304	arg1	content					1306:1312	SPIO microsphere content	1289:1312	SPIO microsphere content	1289:1312	RESULTS Increased r2* relaxivity was observed with incremental increases of SPIO microsphere content.
26313619	12	34	theme	MR	1850:1851	arg1	imaging					1853:1859	CONCLUSION MR imaging	1839:1859	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO	1839:1921	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	5	35	theme	yttrium	900:906	arg1	microspheres					908:919	5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres	864:919	5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres	864:919	For quantitative studies, doses of 5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres were infused into 24 rats (six rats per group).
26313619	0	36	from	Quantification	49:62	arg1	Model					115:119	a Rodent Model	106:119	a Rodent Model	106:119	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.
26313619	3	37	theme	*	472:472	arg1	relaxivity					474:483	The r2* relaxivity	466:483	The r2* relaxivity for each of four microsphere SPIO compositions	466:530	The r2* relaxivity for each of four microsphere SPIO compositions was determined from 32 phantoms constructed with agarose gel and in eight concentrations from each of the four compositions.
26313619	0	38	theme	Imaging	41:47	arg1	Quantification					49:62	MR Imaging Quantification	38:62	MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model	38:119	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.
26313619	0	39	theme	SPIO-labeled	0:11	arg1	Microspheres					21:32	SPIO-labeled Yttrium Microspheres	0:32	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.	0:120	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.
26313619	1	40	theme	magnetic	295:302	arg1	MR					315:316	MR	315:316	MR	315:316	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	1	40	theme	magnetic	295:302	arg1	resonance					304:312	magnetic resonance	295:312	magnetic resonance (MR) imaging in phantom and rodent models	295:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	9	41	theme	linear	1483:1488	arg1	range					1490:1494	a broader linear range	1473:1494	a broader linear range than observed for the other three compositions	1473:1541	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	2	42	theme	Animal	435:440	arg1	Care					442:445	the institutional Animal Care and Use Committee	417:463	Care	442:445	MATERIALS AND METHODS Animal model studies were approved by the institutional Animal Care and Use Committee.
26313619	7	43	theme	intraclass	1100:1109	arg1	correlation					1111:1121	intraclass correlation	1100:1121	intraclass correlation	1100:1121	Pearson correlation, analysis of variance, and intraclass correlation analyses were performed to compare MR imaging measurements in phantoms and animal models.
26313619	9	44	dep	range	1490:1494	arg1	observed					1501:1508	observed	1501:1508	observed for the other three compositions	1501:1541	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	11	45	dep	coefficient	1809:1819	arg1	P					1828:1828	P < .001	1828:1835	P < .001	1828:1835	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	11	45	dep	coefficient	1809:1819	arg1	0.98					1822:1825	0.98	1822:1825	0.98	1822:1825	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	0	46	theme	MR	38:39	arg1	Quantification					49:62	MR Imaging Quantification	38:62	MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model	38:119	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.
26313619	9	47	theme	R2	1315:1316	arg1	measurements					1319:1330	R2* measurements	1315:1330	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration	1315:1387	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	6	48	theme	*	982:982	arg1	measurements					984:995	MR imaging R2* measurements	969:995	MR imaging R2* measurements	969:995	MR imaging R2* measurements were used to quantify the dose delivered to each liver.
26313619	0	49	theme	Transcatheter	67:79	arg1	Delivery					94:101	Transcatheter Intrahepatic Delivery	67:101	Transcatheter Intrahepatic Delivery in a Rodent Model	67:119	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.
26313619	11	50	theme	intraclass	1786:1795	arg1	coefficient					1809:1819	intraclass correlation coefficient	1786:1819	intraclass correlation coefficient	1786:1819	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	1	51	with	microspheres	206:217	arg1	material					282:289	superparamagnetic iron oxide (SPIO) material	246:289	superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	246:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	1	51	with	microspheres	206:217	arg1	amounts					235:241	increasing amounts	224:241	increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	224:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	9	52	theme	other	1518:1522	arg1	compositions					1530:1541	the other three compositions	1514:1541	the other three compositions	1514:1541	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	9	53	dep	=	1450:1450	arg1	1.00					1452:1455	1.00	1452:1455	1.00	1452:1455	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	10	54	theme	SPIO	1632:1635	arg1	content					1637:1643	increasing microsphere SPIO content	1609:1643	increasing microsphere SPIO content	1609:1643	Microspheres were heterogeneously distributed within each liver; increasing microsphere SPIO content produced marked signal voids.
26313619	1	55	theme	superparamagnetic	246:262	arg1	material					282:289	superparamagnetic iron oxide (SPIO) material	246:289	superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	246:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	3	56	theme	SPIO	514:517	arg1	compositions					519:530	four microsphere SPIO compositions	497:530	four microsphere SPIO compositions	497:530	The r2* relaxivity for each of four microsphere SPIO compositions was determined from 32 phantoms constructed with agarose gel and in eight concentrations from each of the four compositions.
26313619	9	57	theme	%	1340:1340	arg1	concentration					1375:1387	the 2% SPIO-labeled yttrium microsphere concentration	1335:1387	the 2% SPIO-labeled yttrium microsphere concentration	1335:1387	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	10	58	theme	signal	1661:1666	arg1	voids					1668:1672	marked signal voids	1654:1672	marked signal voids	1654:1672	Microspheres were heterogeneously distributed within each liver; increasing microsphere SPIO content produced marked signal voids.
26313619	9	59	theme	yttrium	1355:1361	arg1	concentration					1375:1387	the 2% SPIO-labeled yttrium microsphere concentration	1335:1387	the 2% SPIO-labeled yttrium microsphere concentration	1335:1387	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	12	60	theme	in	1949:1950	arg1	biodistribution					1970:1984	in vivo intrahepatic biodistribution	1949:1984	in vivo intrahepatic biodistribution	1949:1984	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	1	61	theme	material	282:289	arg1	material					282:289	superparamagnetic iron oxide (SPIO) material	246:289	superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	246:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	1	61	theme	material	282:289	arg1	amounts					235:241	increasing amounts	224:241	increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	224:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	12	62	theme	2	1915:1915	arg1	%					1916:1916	%	1916:1916	%	1916:1916	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	9	63	theme	P	1458:1458	arg1	<					1460:1460	P < .001	1458:1465	P < .001	1458:1465	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	4	64	theme	Intrahepatic	657:668	arg1	procedures					693:702	Intrahepatic transcatheter infusion procedures	657:702	Intrahepatic transcatheter infusion procedures	657:702	Intrahepatic transcatheter infusion procedures were performed in rats by using each of the four compositions before MR imaging to visualize distributions within the liver.
26313619	7	65	theme	imaging	1161:1167	arg1	measurements					1169:1180	MR imaging measurements	1158:1180	MR imaging measurements	1158:1180	Pearson correlation, analysis of variance, and intraclass correlation analyses were performed to compare MR imaging measurements in phantoms and animal models.
26313619	1	66	theme	yttrium	198:204	arg1	microspheres					206:217	labeling yttrium microspheres	189:217	labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	189:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	12	67	theme	yttrium	1881:1887	arg1	microspheres					1889:1900	yttrium microspheres	1881:1900	yttrium microspheres labeled with 2% SPIO	1881:1921	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	4	68	theme	infusion	684:691	arg1	procedures					693:702	Intrahepatic transcatheter infusion procedures	657:702	Intrahepatic transcatheter infusion procedures	657:702	Intrahepatic transcatheter infusion procedures were performed in rats by using each of the four compositions before MR imaging to visualize distributions within the liver.
26313619	7	69	dep	correlation	1061:1071	arg1	analyses					1123:1130	analyses	1123:1130	analyses	1123:1130	Pearson correlation, analysis of variance, and intraclass correlation analyses were performed to compare MR imaging measurements in phantoms and animal models.
26313619	10	70	theme	increasing	1609:1618	arg1	content					1637:1643	increasing microsphere SPIO content	1609:1643	increasing microsphere SPIO content	1609:1643	Microspheres were heterogeneously distributed within each liver; increasing microsphere SPIO content produced marked signal voids.
26313619	9	71	theme	phantom	1421:1427	arg1	concentrations					1429:1442	known phantom concentrations	1415:1442	known phantom concentrations	1415:1442	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	2	72	theme	Animal	379:384	arg1	studies					392:398	Animal model studies	379:398	Animal model studies	379:398	MATERIALS AND METHODS Animal model studies were approved by the institutional Animal Care and Use Committee.
26313619	11	73	theme	microsphere	1725:1735	arg1	delivery					1737:1744	2% SPIO-labeled yttrium microsphere delivery	1701:1744	2% SPIO-labeled yttrium microsphere delivery	1701:1744	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	1	74	theme	microspheres	206:217	arg1	impacts					178:184	the qualitative and quantitative impacts	145:184	the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	145:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	12	75	theme	R2	1861:1862	arg1	measurements					1865:1876	CONCLUSION MR imaging R2* measurements	1839:1876	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO	1839:1921	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	8	76	theme	Increased	1221:1229	arg1	relaxivity					1235:1244	RESULTS Increased r2* relaxivity	1213:1244	RESULTS Increased r2* relaxivity	1213:1244	RESULTS Increased r2* relaxivity was observed with incremental increases of SPIO microsphere content.
26313619	11	77	theme	infused	1772:1778	arg1	dose					1780:1783	infused dose	1772:1783	infused dose	1772:1783	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	5	78	theme	2	884:884	arg1	%					885:885	%	885:885	%	885:885	For quantitative studies, doses of 5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres were infused into 24 rats (six rats per group).
26313619	8	79	theme	*	1233:1233	arg1	relaxivity					1235:1244	RESULTS Increased r2* relaxivity	1213:1244	RESULTS Increased r2* relaxivity	1213:1244	RESULTS Increased r2* relaxivity was observed with incremental increases of SPIO microsphere content.
26313619	5	80	theme	SPIO-labeled	887:898	arg1	microspheres					908:919	5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres	864:919	5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres	864:919	For quantitative studies, doses of 5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres were infused into 24 rats (six rats per group).
26313619	6	81	theme	R2	980:981	arg1	measurements					984:995	MR imaging R2* measurements	969:995	MR imaging R2* measurements	969:995	MR imaging R2* measurements were used to quantify the dose delivered to each liver.
26313619	5	82	dep	rats	942:945	arg1	rats					952:955	six rats	948:955	six rats per group	948:965	For quantitative studies, doses of 5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres were infused into 24 rats (six rats per group).
26313619	0	83	theme	Intrahepatic	81:92	arg1	Delivery					94:101	Transcatheter Intrahepatic Delivery	67:101	Transcatheter Intrahepatic Delivery in a Rodent Model	67:119	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.
26313619	1	84	theme	qualitative	149:159	arg1	impacts					178:184	the qualitative and quantitative impacts	145:184	the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	145:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	6	85	theme	MR	969:970	arg1	measurements					984:995	MR imaging R2* measurements	969:995	MR imaging R2* measurements	969:995	MR imaging R2* measurements were used to quantify the dose delivered to each liver.
26313619	12	86	theme	intrahepatic	1957:1968	arg1	biodistribution					1970:1984	in vivo intrahepatic biodistribution	1949:1984	in vivo intrahepatic biodistribution	1949:1984	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	11	87	theme	*	1677:1677	arg1	measurements					1685:1696	R2*-based measurements	1675:1696	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery	1675:1744	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	7	88	theme	MR	1158:1159	arg1	measurements					1169:1180	MR imaging measurements	1158:1180	MR imaging measurements	1158:1180	Pearson correlation, analysis of variance, and intraclass correlation analyses were performed to compare MR imaging measurements in phantoms and animal models.
26313619	1	89	theme	rodent	342:347	arg1	models					349:354	phantom and rodent models	330:354	phantom and rodent models	330:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	12	90	theme	CONCLUSION	1839:1848	arg1	imaging					1853:1859	CONCLUSION MR imaging	1839:1859	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO	1839:1921	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	1	91	theme	quantitative	165:176	arg1	impacts					178:184	the qualitative and quantitative impacts	145:184	the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	145:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	11	92	theme	SPIO-labeled	1704:1715	arg1	delivery					1737:1744	2% SPIO-labeled yttrium microsphere delivery	1701:1744	2% SPIO-labeled yttrium microsphere delivery	1701:1744	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	0	93	theme	Rodent	108:113	arg1	Model					115:119	a Rodent Model	106:119	a Rodent Model	106:119	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.
26313619	12	94	theme	imaging	1853:1859	arg1	measurements					1865:1876	CONCLUSION MR imaging R2* measurements	1839:1876	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO	1839:1921	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	4	95	theme	MR	773:774	arg1	imaging					776:782	MR imaging	773:782	MR imaging	773:782	Intrahepatic transcatheter infusion procedures were performed in rats by using each of the four compositions before MR imaging to visualize distributions within the liver.
26313619	2	96	dep	MATERIALS	357:365	arg1	studies					392:398	Animal model studies	379:398	Animal model studies	379:398	MATERIALS AND METHODS Animal model studies were approved by the institutional Animal Care and Use Committee.
26313619	11	97	theme	2	1701:1701	arg1	%					1702:1702	%	1702:1702	%	1702:1702	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	11	98	dep	correlated	1756:1765	arg1	coefficient					1809:1819	intraclass correlation coefficient	1786:1819	intraclass correlation coefficient	1786:1819	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	6	99	used	used	1002:1005	arg2	measurements					984:995	MR imaging R2* measurements	969:995	MR imaging R2* measurements	969:995	MR imaging R2* measurements were used to quantify the dose delivered to each liver.
26313619	8	100	theme	content	1306:1312	arg1	increases					1276:1284	incremental increases	1264:1284	incremental increases of SPIO microsphere content	1264:1312	RESULTS Increased r2* relaxivity was observed with incremental increases of SPIO microsphere content.
26313619	3	101	from	each	626:629	arg1	concentrations					606:619	eight concentrations	600:619	eight concentrations from each of the four compositions	600:654	The r2* relaxivity for each of four microsphere SPIO compositions was determined from 32 phantoms constructed with agarose gel and in eight concentrations from each of the four compositions.
26313619	0	102	theme	Yttrium	13:19	arg1	Microspheres					21:32	SPIO-labeled Yttrium Microspheres	0:32	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.	0:120	SPIO-labeled Yttrium Microspheres for MR Imaging Quantification of Transcatheter Intrahepatic Delivery in a Rodent Model.
26313619	9	103	theme	broader	1475:1481	arg1	range					1490:1494	a broader linear range	1473:1494	a broader linear range than observed for the other three compositions	1473:1541	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	2	104	theme	Use	451:453	arg1	Committee					455:463	the institutional Animal Care and Use Committee	417:463	Committee	455:463	MATERIALS AND METHODS Animal model studies were approved by the institutional Animal Care and Use Committee.
26313619	5	105	dep	mg	881:882	arg1	20					878:879	20	878:879	20	878:879	For quantitative studies, doses of 5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres were infused into 24 rats (six rats per group).
26313619	5	105	dep	mg	881:882	arg1	15					871:872	15	871:872	15	871:872	For quantitative studies, doses of 5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres were infused into 24 rats (six rats per group).
26313619	2	106	theme	model	386:390	arg1	studies					392:398	Animal model studies	379:398	Animal model studies	379:398	MATERIALS AND METHODS Animal model studies were approved by the institutional Animal Care and Use Committee.
26313619	5	107	theme	quantitative	833:844	arg1	studies					846:852	quantitative studies	833:852	quantitative studies	833:852	For quantitative studies, doses of 5, 10, 15, or 20 mg 2% SPIO-labeled yttrium microspheres were infused into 24 rats (six rats per group).
26313619	1	108	theme	resonance	304:312	arg1	imaging					319:325	magnetic resonance (MR) imaging	295:325	magnetic resonance (MR) imaging in phantom and rodent models	295:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	2	109	theme	institutional	421:433	arg1	Care					442:445	the institutional Animal Care and Use Committee	417:463	Care	442:445	MATERIALS AND METHODS Animal model studies were approved by the institutional Animal Care and Use Committee.
26313619	7	110	theme	variance	1086:1093	arg1	correlation					1111:1121	intraclass correlation	1100:1121	intraclass correlation	1100:1121	Pearson correlation, analysis of variance, and intraclass correlation analyses were performed to compare MR imaging measurements in phantoms and animal models.
26313619	7	110	theme	variance	1086:1093	arg1	analysis					1074:1081	analysis	1074:1081	analysis of variance	1074:1093	Pearson correlation, analysis of variance, and intraclass correlation analyses were performed to compare MR imaging measurements in phantoms and animal models.
26313619	7	110	theme	variance	1086:1093	arg1	correlation					1061:1071	Pearson correlation	1053:1071	Pearson correlation	1053:1071	Pearson correlation, analysis of variance, and intraclass correlation analyses were performed to compare MR imaging measurements in phantoms and animal models.
26313619	1	111	theme	oxide	269:273	arg1	material					282:289	superparamagnetic iron oxide (SPIO) material	246:289	superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	246:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	1	112	theme	iron	264:267	arg1	oxide					269:273	iron oxide	264:273	superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models	246:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	1	112	theme	iron	264:267	arg1	SPIO					276:279	SPIO	276:279	SPIO	276:279	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	9	113	theme	*	1317:1317	arg1	measurements					1319:1330	R2* measurements	1315:1330	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration	1315:1387	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	12	114	dep	in	1949:1950	arg1	vivo					1952:1955	vivo	1952:1955	vivo	1952:1955	CONCLUSION MR imaging R2* measurements of yttrium microspheres labeled with 2% SPIO can quantitatively depict in vivo intrahepatic biodistribution in a rat model.
26313619	9	115	dep	correlated	1399:1408	arg1	R					1445:1445	R(2)	1445:1448	R(2) = 1.00	1445:1455	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	11	116	theme	correlation	1797:1807	arg1	coefficient					1809:1819	intraclass correlation coefficient	1786:1819	intraclass correlation coefficient	1786:1819	R2*-based measurements of 2% SPIO-labeled yttrium microsphere delivery were well correlated with infused dose (intraclass correlation coefficient, 0.98; P < .001).
26313619	1	117	theme	phantom	330:336	arg1	models					349:354	phantom and rodent models	330:354	phantom and rodent models	330:354	PURPOSE To investigate the qualitative and quantitative impacts of labeling yttrium microspheres with increasing amounts of superparamagnetic iron oxide (SPIO) material for magnetic resonance (MR) imaging in phantom and rodent models.
26313619	9	118	dep	R	1445:1445	arg1	<					1460:1460	P < .001	1458:1465	P < .001	1458:1465	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	10	119	theme	microsphere	1620:1630	arg1	content					1637:1643	increasing microsphere SPIO content	1609:1643	increasing microsphere SPIO content	1609:1643	Microspheres were heterogeneously distributed within each liver; increasing microsphere SPIO content produced marked signal voids.
26313619	8	120	theme	SPIO	1289:1292	arg1	content					1306:1312	SPIO microsphere content	1289:1312	SPIO microsphere content	1289:1312	RESULTS Increased r2* relaxivity was observed with incremental increases of SPIO microsphere content.
26313619	9	121	theme	2	1339:1339	arg1	%					1340:1340	%	1340:1340	%	1340:1340	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
26313619	7	122	theme	animal	1198:1203	arg1	models					1205:1210	animal models	1198:1210	animal models	1198:1210	Pearson correlation, analysis of variance, and intraclass correlation analyses were performed to compare MR imaging measurements in phantoms and animal models.
26313619	9	123	theme	SPIO-labeled	1342:1353	arg1	concentration					1375:1387	the 2% SPIO-labeled yttrium microsphere concentration	1335:1387	the 2% SPIO-labeled yttrium microsphere concentration	1335:1387	R2* measurements of the 2% SPIO-labeled yttrium microsphere concentration were well correlated with known phantom concentrations (R(2) = 1.00, P < .001) over a broader linear range than observed for the other three compositions.
28007381	3	0	theme	RV	469:470	arg1	Polarstern					472:481	the RV Polarstern	465:481	the RV Polarstern	465:481	Microplastics were sampled from sub-surface waters using the underway system of the RV Polarstern.
28007381	2	1	from	composition	286:296	arg1	a					323:323	a	323:323	a	323:323	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	2	1	from	composition	286:296	arg1	Ocean					314:318	the Atlantic Ocean	301:318	the Atlantic Ocean	301:318	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	4	2	theme	Potential	484:492	arg1	microplastics					494:506	Potential microplastics	484:506	Potential microplastics	484:506	Potential microplastics were isolated from samples and FT-IR spectroscopy was used to identify polymer types.
28007381	1	3	theme	global	147:152	arg1	concern					154:160	a global concern	145:160	a global concern due to the potential threat they pose to marine organisms	145:218	Microplastics in the world's oceans are a global concern due to the potential threat they pose to marine organisms.
28007381	1	3	theme	global	147:152	arg1	Microplastics					105:117	Microplastics	105:117	Microplastics in the world's oceans	105:139	Microplastics in the world's oceans are a global concern due to the potential threat they pose to marine organisms.
28007381	9	4	from	difference	1005:1014	arg1	abundance					1032:1040	microplastic abundance	1019:1040	microplastic abundance	1019:1040	Of the 76 samples, 14 were from the Benguela upwelling and there was no statistically significant difference in microplastic abundance between upwelled and non-upwelled sites.
28007381	6	5	theme	polyamide	749:757	arg1	majority					673:680	The majority	669:680	The majority of microplastics	669:697	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	6	5	theme	polyamide	749:757	arg1	%					783:783	43%	781:783	43%	781:783	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	6	5	theme	polyamide	749:757	arg1	polyesters					718:727	polyesters	718:727	polyesters (49%)	718:733	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	6	5	theme	polyamide	749:757	arg1	%					732:732	49%	730:732	49%	730:732	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	6	5	theme	polyamide	749:757	arg1	blends					739:744	blends	739:744	blends of polyamide or acrylic/polyester (43%)	739:784	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	0	6	theme	Atlantic	89:96	arg1	Ocean					98:102	the Atlantic Ocean	85:102	the Atlantic Ocean	85:102	Microplastic abundance, distribution and composition along a latitudinal gradient in the Atlantic Ocean.
28007381	9	7	theme	upwelled	1050:1057	arg1	sites					1076:1080	upwelled and non-upwelled sites	1050:1080	upwelled and non-upwelled sites	1050:1080	Of the 76 samples, 14 were from the Benguela upwelling and there was no statistically significant difference in microplastic abundance between upwelled and non-upwelled sites.
28007381	5	8	theme	particles	601:609	arg1	polymers					659:666	synthetic polymers	649:666	synthetic polymers	649:666	Of the particles analysed, 63% were rayon and 37% were synthetic polymers.
28007381	5	8	theme	particles	601:609	arg1	rayon					630:634	rayon	630:634	rayon	630:634	Of the particles analysed, 63% were rayon and 37% were synthetic polymers.
28007381	4	9	theme	polymer	579:585	arg1	types					587:591	polymer types	579:591	polymer types	579:591	Potential microplastics were isolated from samples and FT-IR spectroscopy was used to identify polymer types.
28007381	9	10	theme	significant	993:1003	arg1	difference					1005:1014	no statistically significant difference	976:1014	no statistically significant difference in microplastic abundance between upwelled and non-upwelled sites	976:1080	Of the 76 samples, 14 were from the Benguela upwelling and there was no statistically significant difference in microplastic abundance between upwelled and non-upwelled sites.
28007381	1	11	theme	due	162:164	arg1	concern					154:160	a global concern	145:160	a global concern due to the potential threat they pose to marine organisms	145:218	Microplastics in the world's oceans are a global concern due to the potential threat they pose to marine organisms.
28007381	1	11	theme	due	162:164	arg1	Microplastics					105:117	Microplastics	105:117	Microplastics in the world's oceans	105:139	Microplastics in the world's oceans are a global concern due to the potential threat they pose to marine organisms.
28007381	2	12	theme	Atlantic	305:312	arg1	Ocean					314:318	the Atlantic Ocean	301:318	the Atlantic Ocean	301:318	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	0	13	theme	Microplastic	0:11	arg1	abundance					13:21	Microplastic abundance	0:21	Microplastic abundance	0:21	Microplastic abundance, distribution and composition along a latitudinal gradient in the Atlantic Ocean.
28007381	2	14	theme	Biscay	350:355	arg1	Bay					343:345	the Bay	339:345	the Bay of Biscay to Cape Town, South Africa	339:382	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	8	15	from	abundance	848:856	arg1	Ocean					874:878	the Atlantic Ocean	861:878	the Atlantic Ocean	861:878	Average microplastic abundance in the Atlantic Ocean was 1.15±1.45particlesm-3.
28007381	5	16	theme	synthetic	649:657	arg1	polymers					659:666	synthetic polymers	649:666	synthetic polymers	649:666	Of the particles analysed, 63% were rayon and 37% were synthetic polymers.
28007381	4	17	attach	isolated	513:520	arg2	microplastics					494:506	Potential microplastics	484:506	Potential microplastics	484:506	Potential microplastics were isolated from samples and FT-IR spectroscopy was used to identify polymer types.
28007381	4	17	attach	isolated	513:520	arg1	samples					527:533	samples	527:533	samples	527:533	Potential microplastics were isolated from samples and FT-IR spectroscopy was used to identify polymer types.
28007381	9	18	theme	microplastic	1019:1030	arg1	abundance					1032:1040	microplastic abundance	1019:1040	microplastic abundance	1019:1040	Of the 76 samples, 14 were from the Benguela upwelling and there was no statistically significant difference in microplastic abundance between upwelled and non-upwelled sites.
28007381	1	19	theme	potential	173:181	arg1	threat					183:188	the potential threat	169:188	the potential threat they pose to marine organisms	169:218	Microplastics in the world's oceans are a global concern due to the potential threat they pose to marine organisms.
28007381	2	20	from	abundance	258:266	arg1	a					323:323	a	323:323	a	323:323	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	2	20	from	abundance	258:266	arg1	Ocean					314:318	the Atlantic Ocean	301:318	the Atlantic Ocean	301:318	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	9	21	theme	non-upwelled	1063:1074	arg1	sites					1076:1080	upwelled and non-upwelled sites	1050:1080	upwelled and non-upwelled sites	1050:1080	Of the 76 samples, 14 were from the Benguela upwelling and there was no statistically significant difference in microplastic abundance between upwelled and non-upwelled sites.
28007381	2	22	dep	a	323:323	arg1	transect					325:332	transect	325:332	transect	325:332	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	4	23	theme	FT-IR	539:543	arg1	spectroscopy					545:556	FT-IR spectroscopy	539:556	FT-IR spectroscopy	539:556	Potential microplastics were isolated from samples and FT-IR spectroscopy was used to identify polymer types.
28007381	9	24	theme	Benguela	943:950	arg1	upwelling					952:960	the Benguela upwelling	939:960	the Benguela upwelling	939:960	Of the 76 samples, 14 were from the Benguela upwelling and there was no statistically significant difference in microplastic abundance between upwelled and non-upwelled sites.
28007381	3	25	theme	sub-surface	417:427	arg1	waters					429:434	sub-surface waters	417:434	sub-surface waters using the underway system of the RV Polarstern	417:481	Microplastics were sampled from sub-surface waters using the underway system of the RV Polarstern.
28007381	9	26	theme	samples	917:923	arg1	upwelling					952:960	the Benguela upwelling	939:960	the Benguela upwelling	939:960	Of the 76 samples, 14 were from the Benguela upwelling and there was no statistically significant difference in microplastic abundance between upwelled and non-upwelled sites.
28007381	0	27	from	gradient	73:80	arg1	Ocean					98:102	the Atlantic Ocean	85:102	the Atlantic Ocean	85:102	Microplastic abundance, distribution and composition along a latitudinal gradient in the Atlantic Ocean.
28007381	8	28	theme	microplastic	835:846	arg1	abundance					848:856	Average microplastic abundance	827:856	Average microplastic abundance in the Atlantic Ocean	827:878	Average microplastic abundance in the Atlantic Ocean was 1.15±1.45particlesm-3.
28007381	8	29	theme	Average	827:833	arg1	abundance					848:856	Average microplastic abundance	827:856	Average microplastic abundance in the Atlantic Ocean	827:878	Average microplastic abundance in the Atlantic Ocean was 1.15±1.45particlesm-3.
28007381	2	30	dep	Africa	377:382	arg1	South					371:375	South	371:375	South	371:375	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	3	31	theme	Polarstern	472:481	arg1	system					455:460	the underway system	442:460	the underway system of the RV Polarstern	442:481	Microplastics were sampled from sub-surface waters using the underway system of the RV Polarstern.
28007381	2	32	theme	microplastic	245:256	arg1	abundance					258:266	microplastic abundance	245:266	microplastic abundance	245:266	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	3	33	theme	underway	446:453	arg1	system					455:460	the underway system	442:460	the underway system of the RV Polarstern	442:481	Microplastics were sampled from sub-surface waters using the underway system of the RV Polarstern.
28007381	1	34	from	Microplastics	105:117	arg1	oceans					134:139	the world's oceans	122:139	the world's oceans	122:139	Microplastics in the world's oceans are a global concern due to the potential threat they pose to marine organisms.
28007381	4	35	used	used	562:565	arg2	spectroscopy					545:556	FT-IR spectroscopy	539:556	FT-IR spectroscopy	539:556	Potential microplastics were isolated from samples and FT-IR spectroscopy was used to identify polymer types.
28007381	2	36	dep	Biscay	350:355	arg1	Africa					377:382	Africa	377:382	Africa	377:382	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	2	36	dep	Biscay	350:355	arg1	to					357:358	to	357:358	to	357:358	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	8	37	theme	Atlantic	865:872	arg1	Ocean					874:878	the Atlantic Ocean	861:878	the Atlantic Ocean	861:878	Average microplastic abundance in the Atlantic Ocean was 1.15±1.45particlesm-3.
28007381	1	38	theme	marine	203:208	arg1	organisms					210:218	marine organisms	203:218	marine organisms	203:218	Microplastics in the world's oceans are a global concern due to the potential threat they pose to marine organisms.
28007381	6	39	theme	acrylic/polyester	762:778	arg1	majority					673:680	The majority	669:680	The majority of microplastics	669:697	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	6	39	theme	acrylic/polyester	762:778	arg1	%					783:783	43%	781:783	43%	781:783	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	6	39	theme	acrylic/polyester	762:778	arg1	polyesters					718:727	polyesters	718:727	polyesters (49%)	718:733	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	6	39	theme	acrylic/polyester	762:778	arg1	%					732:732	49%	730:732	49%	730:732	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	6	39	theme	acrylic/polyester	762:778	arg1	blends					739:744	blends	739:744	blends of polyamide or acrylic/polyester (43%)	739:784	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	6	40	theme	microplastics	685:697	arg1	majority					673:680	The majority	669:680	The majority of microplastics	669:697	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	6	40	theme	microplastics	685:697	arg1	polyesters					718:727	polyesters	718:727	polyesters (49%)	718:733	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	6	40	theme	microplastics	685:697	arg1	blends					739:744	blends	739:744	blends of polyamide or acrylic/polyester (43%)	739:784	The majority of microplastics were identified as polyesters (49%) and blends of polyamide or acrylic/polyester (43%).
28007381	2	41	from	distribution	269:280	arg1	a					323:323	a	323:323	a	323:323	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	2	41	from	distribution	269:280	arg1	Ocean					314:318	the Atlantic Ocean	301:318	the Atlantic Ocean	301:318	This study investigated microplastic abundance, distribution and composition in the Atlantic Ocean on a transect from the Bay of Biscay to Cape Town, South Africa.
28007381	0	42	theme	latitudinal	61:71	arg1	gradient					73:80	a latitudinal gradient	59:80	a latitudinal gradient in the Atlantic Ocean	59:102	Microplastic abundance, distribution and composition along a latitudinal gradient in the Atlantic Ocean.
28007381	1	43	dep	threat	183:188	arg1	pose					195:198	pose	195:198	pose to marine organisms	195:218	Microplastics in the world's oceans are a global concern due to the potential threat they pose to marine organisms.
29078191	4	0	theme	electron	813:820	arg1	FESEM					834:838	FESEM	834:838	FESEM	834:838	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	0	theme	electron	813:820	arg1	microscopy					822:831	field emission scanning electron microscopy	789:831	field emission scanning electron microscopy (FESEM)	789:839	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	9	1	theme	maximum	1747:1753	arg1	%					1853:1853	99.6%	1849:1853	99.6%	1849:1853	The maximum Cr (VI) removal results by using CA/Ti-TEPA membrane for four washing/regeneration cycles are 99.6%, 99.5%, 98.6% and, 96.6%, respectively.
29078191	9	1	theme	maximum	1747:1753	arg1	Cr					1755:1756	The maximum Cr (VI) removal results by using CA/Ti-TEPA membrane for four washing/regeneration cycles	1743:1843	The maximum Cr (VI) removal results by using CA/Ti-TEPA membrane for four washing/regeneration cycles	1743:1843	The maximum Cr (VI) removal results by using CA/Ti-TEPA membrane for four washing/regeneration cycles are 99.6%, 99.5%, 98.6% and, 96.6%, respectively.
29078191	2	2	theme	prepared	320:327	arg1	additive					377:384	additive	377:384	additive	377:384	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	2	2	theme	prepared	320:327	arg1	samples					349:355	The prepared amine modified TiO2 samples	316:355	The prepared amine modified TiO2 samples	316:355	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	6	3	theme	TiO2	1335:1338	arg1	composite					1340:1348	amine modified TiO2 composite	1320:1348	amine modified TiO2 composite	1320:1348	Incorporation of amine modified TiO2 composite to the CA polymer was found to improve the fouling and removal characteristics of the membranes during the chromium ultrafiltration process.
29078191	8	4	theme	washing/regeneration	1599:1618	arg1	cycle					1620:1624	The washing/regeneration cycle	1595:1624	The washing/regeneration cycle	1595:1624	The washing/regeneration cycle results in this study described as an essential part for prospect industrial applications of the prepared membranes.
29078191	5	5	dep	CA/U-Ti	1171:1177	arg1	i.e.					1166:1169	i.e.	1166:1169	i.e.	1166:1169	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	4	6	theme	morphology	1031:1040	arg1	terms					966:970	terms	966:970	terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance	966:1081	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	7	theme	emission	795:802	arg1	FESEM					834:838	FESEM	834:838	FESEM	834:838	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	7	theme	emission	795:802	arg1	microscopy					822:831	field emission scanning electron microscopy	789:831	field emission scanning electron microscopy (FESEM)	789:839	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	8	theme	hydrophilicity	1043:1056	arg1	terms					966:970	terms	966:970	terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance	966:1081	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	5	9	theme	ion	1284:1286	arg1	concentration					1288:1300	metal ion concentration	1278:1300	metal ion concentration	1278:1300	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	7	10	theme	Cr	1532:1533	arg1	result					1522:1527	The maximum removal efficiency result	1491:1527	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane	1491:1582	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	7	10	theme	Cr	1532:1533	arg1	%					1592:1592	99.8%	1588:1592	99.8%	1588:1592	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	0	11	theme	acetate	115:121	arg1	membranes					123:131	modified cellulose acetate membranes	96:131	modified cellulose acetate membranes	96:131	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2 nanoparticles modified cellulose acetate membranes.
29078191	3	12	theme	acetate	556:562	arg1	polymer					569:575	cellulose acetate (CA) polymer	546:575	cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique	546:683	Modified membranes were prepared from cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique.
29078191	4	13	theme	stability	1020:1028	arg1	terms					966:970	terms	966:970	terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance	966:1081	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	2	14	with	membranes	415:423	arg1	capacity					439:446	enhanced capacity	430:446	enhanced capacity	430:446	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	7	15	theme	removal	1503:1509	arg1	result					1522:1527	The maximum removal efficiency result	1491:1527	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane	1491:1582	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	7	15	theme	removal	1503:1509	arg1	%					1592:1592	99.8%	1588:1592	99.8%	1588:1592	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	6	16	theme	chromium	1457:1464	arg1	process					1482:1488	the chromium ultrafiltration process	1453:1488	the chromium ultrafiltration process	1453:1488	Incorporation of amine modified TiO2 composite to the CA polymer was found to improve the fouling and removal characteristics of the membranes during the chromium ultrafiltration process.
29078191	4	17	theme	water	842:846	arg1	angle					856:860	water contact angle	842:860	water contact angle (WCA)	842:866	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	17	theme	water	842:846	arg1	WCA					863:865	WCA	863:865	WCA	863:865	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	18	theme	infrared	704:711	arg1	FTIR					727:730	FTIR	727:730	FTIR	727:730	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	18	theme	infrared	704:711	arg1	spectroscopy					713:724	infrared spectroscopy	704:724	infrared spectroscopy (FTIR)	704:731	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	19	dep	spectroscopy	713:724	arg1	studies					915:921	studies	915:921	studies	915:921	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	5	20	dep	Cr	1116:1117	arg1	VI					1120:1121	VI	1120:1121	VI	1120:1121	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	5	20	dep	Cr	1116:1117	arg1	efficiency					1136:1145	ion removal efficiency	1124:1145	Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes	1116:1251	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	0	21	theme	aqueous	35:41	arg1	solutions					43:51	aqueous solutions	35:51	aqueous solutions	35:51	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2 nanoparticles modified cellulose acetate membranes.
29078191	1	22	theme	impregnation	294:305	arg1	process					307:313	impregnation process	294:313	impregnation process	294:313	In this work, TiO2 nanoparticles (NPs) were modified using tetraethylenepentamine (TEPA), ethylenediamine (EDA), and hexamethylenetetramine (HMTA) amines using impregnation process.
29078191	2	23	theme	ions	480:483	arg1	removal					460:466	the removal	456:466	the removal of chromium ions from aqueous solution	456:505	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	2	24	theme	chromium	471:478	arg1	ions					480:483	chromium ions	471:483	chromium ions	471:483	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	4	25	theme	performance	1071:1081	arg1	terms					966:970	terms	966:970	terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance	966:1081	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	8	26	theme	essential	1664:1672	arg1	part					1674:1677	an essential part	1661:1677	an essential part for prospect industrial applications of the prepared membranes	1661:1740	The washing/regeneration cycle results in this study described as an essential part for prospect industrial applications of the prepared membranes.
29078191	1	27	theme	TiO2	148:151	arg1	NPs					168:170	NPs	168:170	NPs	168:170	In this work, TiO2 nanoparticles (NPs) were modified using tetraethylenepentamine (TEPA), ethylenediamine (EDA), and hexamethylenetetramine (HMTA) amines using impregnation process.
29078191	1	27	theme	TiO2	148:151	arg1	nanoparticles					153:165	TiO2 nanoparticles	148:165	TiO2 nanoparticles (NPs)	148:171	In this work, TiO2 nanoparticles (NPs) were modified using tetraethylenepentamine (TEPA), ethylenediamine (EDA), and hexamethylenetetramine (HMTA) amines using impregnation process.
29078191	0	28	from	Removal	0:6	arg1	solutions					43:51	aqueous solutions	35:51	aqueous solutions	35:51	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2 nanoparticles modified cellulose acetate membranes.
29078191	4	29	theme	zeta	734:737	arg1	ζ					750:750	ζ	750:750	ζ	750:750	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	29	theme	zeta	734:737	arg1	potential					739:747	zeta potential	734:747	zeta potential (ζ)	734:751	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	30	theme	atomic	873:878	arg1	spectrophotometer					891:907	atomic absorption spectrophotometer	873:907	atomic absorption spectrophotometer (AAS)	873:913	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	30	theme	atomic	873:878	arg1	AAS					910:912	AAS	910:912	AAS	910:912	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	0	31	theme	modified	96:103	arg1	membranes					123:131	modified cellulose acetate membranes	96:131	modified cellulose acetate membranes	96:131	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2 nanoparticles modified cellulose acetate membranes.
29078191	7	32	from	result	1522:1527	arg1	pH					1548:1549	pH	1548:1549	pH of 3.5	1548:1556	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	8	33	theme	industrial	1692:1701	arg1	applications					1703:1714	prospect industrial applications	1683:1714	prospect industrial applications of the prepared membranes	1683:1740	The washing/regeneration cycle results in this study described as an essential part for prospect industrial applications of the prepared membranes.
29078191	2	34	theme	enhanced	430:437	arg1	capacity					439:446	enhanced capacity	430:446	enhanced capacity	430:446	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	0	35	theme	chromium	11:18	arg1	Removal					0:6	Removal	0:6	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2	0:80	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2 nanoparticles modified cellulose acetate membranes.
29078191	5	36	theme	water	1093:1097	arg1	dependent					1258:1266	dependent	1258:1266	dependent	1258:1266	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	5	36	theme	water	1093:1097	arg1	permeability					1099:1110	The pure water permeability	1084:1110	The pure water permeability	1084:1110	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	8	37	theme	membranes	1732:1740	arg1	applications					1703:1714	prospect industrial applications	1683:1714	prospect industrial applications of the prepared membranes	1683:1740	The washing/regeneration cycle results in this study described as an essential part for prospect industrial applications of the prepared membranes.
29078191	3	38	theme	additive	616:623	arg1	TiO2					645:648	polyethylene glycol (PEG) additive, and amine modified TiO2	590:648	polyethylene glycol (PEG) additive, and amine modified TiO2	590:648	Modified membranes were prepared from cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique.
29078191	0	39	dep	chromium	11:18	arg1	ions					25:28	ions	25:28	chromium (VI) ions from aqueous solutions using amine-impregnated TiO2	11:80	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2 nanoparticles modified cellulose acetate membranes.
29078191	0	39	dep	chromium	11:18	arg1	VI					21:22	VI	21:22	VI	21:22	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2 nanoparticles modified cellulose acetate membranes.
29078191	4	40	theme	charge	1004:1009	arg1	terms					966:970	terms	966:970	terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance	966:1081	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	3	41	theme	modified	636:643	arg1	TiO2					645:648	polyethylene glycol (PEG) additive, and amine modified TiO2	590:648	polyethylene glycol (PEG) additive, and amine modified TiO2	590:648	Modified membranes were prepared from cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique.
29078191	4	42	theme	thermo	754:759	arg1	analysis					773:780	thermo gravimetric analysis	754:780	thermo gravimetric analysis (TGA)	754:786	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	42	theme	thermo	754:759	arg1	TGA					783:785	TGA	783:785	TGA	783:785	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	2	43	theme	TiO2	344:347	arg1	additive					377:384	additive	377:384	additive	377:384	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	2	43	theme	TiO2	344:347	arg1	samples					349:355	The prepared amine modified TiO2 samples	316:355	The prepared amine modified TiO2 samples	316:355	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	5	44	theme	removal	1128:1134	arg1	efficiency					1136:1145	ion removal efficiency	1124:1145	Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes	1116:1251	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	8	45	theme	prepared	1723:1730	arg1	membranes					1732:1740	the prepared membranes	1719:1740	the prepared membranes	1719:1740	The washing/regeneration cycle results in this study described as an essential part for prospect industrial applications of the prepared membranes.
29078191	5	46	theme	membranes	1243:1251	arg1	dependent					1258:1266	dependent	1258:1266	dependent	1258:1266	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	5	46	theme	membranes	1243:1251	arg1	Cr					1116:1117	Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes	1116:1251	Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes	1116:1251	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	5	46	theme	membranes	1243:1251	arg1	permeability					1099:1110	The pure water permeability	1084:1110	The pure water permeability	1084:1110	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	2	47	theme	amine	329:333	arg1	additive					377:384	additive	377:384	additive	377:384	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	2	47	theme	amine	329:333	arg1	samples					349:355	The prepared amine modified TiO2 samples	316:355	The prepared amine modified TiO2 samples	316:355	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	6	48	theme	composite	1340:1348	arg1	Incorporation					1303:1315	Incorporation	1303:1315	Incorporation of amine modified TiO2 composite to the CA polymer	1303:1366	Incorporation of amine modified TiO2 composite to the CA polymer was found to improve the fouling and removal characteristics of the membranes during the chromium ultrafiltration process.
29078191	4	49	theme	scanning	804:811	arg1	FESEM					834:838	FESEM	834:838	FESEM	834:838	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	49	theme	scanning	804:811	arg1	microscopy					822:831	field emission scanning electron microscopy	789:831	field emission scanning electron microscopy (FESEM)	789:839	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	50	theme	chemical	975:982	arg1	structure					984:992	chemical structure	975:992	chemical structure	975:992	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	3	51	theme	phase	659:663	arg1	technique					675:683	phase inversion technique	659:683	phase inversion technique	659:683	Modified membranes were prepared from cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique.
29078191	6	52	theme	modified	1326:1333	arg1	composite					1340:1348	amine modified TiO2 composite	1320:1348	amine modified TiO2 composite	1320:1348	Incorporation of amine modified TiO2 composite to the CA polymer was found to improve the fouling and removal characteristics of the membranes during the chromium ultrafiltration process.
29078191	6	53	theme	removal	1405:1411	arg1	characteristics					1413:1427	the fouling and removal characteristics	1389:1427	the fouling and removal characteristics of the membranes	1389:1444	Incorporation of amine modified TiO2 composite to the CA polymer was found to improve the fouling and removal characteristics of the membranes during the chromium ultrafiltration process.
29078191	5	54	dep	unmodified	1154:1163	arg1	CA/U-Ti					1171:1177	CA/U-Ti	1171:1177	CA/U-Ti	1171:1177	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	5	54	dep	unmodified	1154:1163	arg1	CA/Ti-EDA					1216:1224	CA/Ti-EDA	1216:1224	CA/Ti-EDA	1216:1224	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	5	54	dep	unmodified	1154:1163	arg1	CA/Ti-TEPA					1231:1240	CA/Ti-TEPA	1231:1240	CA/Ti-TEPA	1231:1240	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	5	54	dep	unmodified	1154:1163	arg1	CA/Ti-HMTA					1204:1213	CA/Ti-HMTA	1204:1213	CA/Ti-HMTA	1204:1213	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	9	55	theme	removal	1763:1769	arg1	results					1771:1777	removal results	1763:1777	The maximum Cr (VI) removal results by using CA/Ti-TEPA membrane for four washing/regeneration cycles	1743:1843	The maximum Cr (VI) removal results by using CA/Ti-TEPA membrane for four washing/regeneration cycles are 99.6%, 99.5%, 98.6% and, 96.6%, respectively.
29078191	5	56	theme	metal	1278:1282	arg1	concentration					1288:1300	metal ion concentration	1278:1300	metal ion concentration	1278:1300	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	6	57	theme	fouling	1393:1399	arg1	characteristics					1413:1427	the fouling and removal characteristics	1389:1427	the fouling and removal characteristics of the membranes	1389:1444	Incorporation of amine modified TiO2 composite to the CA polymer was found to improve the fouling and removal characteristics of the membranes during the chromium ultrafiltration process.
29078191	4	58	theme	field	789:793	arg1	FESEM					834:838	FESEM	834:838	FESEM	834:838	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	58	theme	field	789:793	arg1	microscopy					822:831	field emission scanning electron microscopy	789:831	field emission scanning electron microscopy (FESEM)	789:839	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	59	theme	thermal	1012:1018	arg1	stability					1020:1028	thermal stability	1012:1028	thermal stability	1012:1028	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	3	60	theme	cellulose	546:554	arg1	acetate					556:562	cellulose acetate	546:562	cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique	546:683	Modified membranes were prepared from cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique.
29078191	3	60	theme	cellulose	546:554	arg1	CA					565:566	CA	565:566	CA	565:566	Modified membranes were prepared from cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique.
29078191	7	61	theme	efficiency	1511:1520	arg1	result					1522:1527	The maximum removal efficiency result	1491:1527	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane	1491:1582	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	7	61	theme	efficiency	1511:1520	arg1	%					1592:1592	99.8%	1588:1592	99.8%	1588:1592	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	4	62	theme	contact	848:854	arg1	angle					856:860	water contact angle	842:860	water contact angle (WCA)	842:866	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	62	theme	contact	848:854	arg1	WCA					863:865	WCA	863:865	WCA	863:865	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	9	63	theme	CA/Ti-TEPA	1788:1797	arg1	membrane					1799:1806	CA/Ti-TEPA membrane	1788:1806	CA/Ti-TEPA membrane for four washing/regeneration cycles	1788:1843	The maximum Cr (VI) removal results by using CA/Ti-TEPA membrane for four washing/regeneration cycles are 99.6%, 99.5%, 98.6% and, 96.6%, respectively.
29078191	7	64	theme	maximum	1495:1501	arg1	result					1522:1527	The maximum removal efficiency result	1491:1527	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane	1491:1582	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	7	64	theme	maximum	1495:1501	arg1	%					1592:1592	99.8%	1588:1592	99.8%	1588:1592	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	0	65	from	solutions	43:51	arg1	chromium					11:18	chromium (VI) ions from aqueous solutions using amine-impregnated TiO2	11:80	chromium (VI) ions from aqueous solutions using amine-impregnated TiO2	11:80	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2 nanoparticles modified cellulose acetate membranes.
29078191	0	65	from	solutions	43:51	arg1	Removal					0:6	Removal	0:6	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2	0:80	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2 nanoparticles modified cellulose acetate membranes.
29078191	6	66	theme	ultrafiltration	1466:1480	arg1	process					1482:1488	the chromium ultrafiltration process	1453:1488	the chromium ultrafiltration process	1453:1488	Incorporation of amine modified TiO2 composite to the CA polymer was found to improve the fouling and removal characteristics of the membranes during the chromium ultrafiltration process.
29078191	2	67	from	solution	498:505	arg1	removal					460:466	the removal	456:466	the removal of chromium ions from aqueous solution	456:505	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	9	68	theme	washing/regeneration	1817:1836	arg1	cycles					1838:1843	four washing/regeneration cycles	1812:1843	four washing/regeneration cycles	1812:1843	The maximum Cr (VI) removal results by using CA/Ti-TEPA membrane for four washing/regeneration cycles are 99.6%, 99.5%, 98.6% and, 96.6%, respectively.
29078191	7	69	from	pH	1548:1549	arg1	result					1522:1527	The maximum removal efficiency result	1491:1527	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane	1491:1582	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	7	69	from	pH	1548:1549	arg1	%					1592:1592	99.8%	1588:1592	99.8%	1588:1592	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	7	69	from	pH	1548:1549	arg1	Cr					1532:1533	Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane	1532:1582	Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane	1532:1582	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	4	70	theme	removal	1063:1069	arg1	performance					1071:1081	removal performance	1063:1081	removal performance	1063:1081	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	3	71	theme	polyethylene	590:601	arg1	glycol					603:608	polyethylene glycol	590:608	polyethylene glycol (PEG)	590:614	Modified membranes were prepared from cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique.
29078191	3	71	theme	polyethylene	590:601	arg1	PEG					611:613	PEG	611:613	PEG	611:613	Modified membranes were prepared from cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique.
29078191	6	72	theme	membranes	1436:1444	arg1	characteristics					1413:1427	the fouling and removal characteristics	1389:1427	the fouling and removal characteristics of the membranes	1389:1444	Incorporation of amine modified TiO2 composite to the CA polymer was found to improve the fouling and removal characteristics of the membranes during the chromium ultrafiltration process.
29078191	4	73	theme	absorption	880:889	arg1	spectrophotometer					891:907	atomic absorption spectrophotometer	873:907	atomic absorption spectrophotometer (AAS)	873:913	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	73	theme	absorption	880:889	arg1	AAS					910:912	AAS	910:912	AAS	910:912	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	0	74	theme	amine-impregnated	59:75	arg1	TiO2					77:80	amine-impregnated TiO2	59:80	amine-impregnated TiO2	59:80	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2 nanoparticles modified cellulose acetate membranes.
29078191	5	75	theme	unmodified	1154:1163	arg1	membranes					1243:1251	the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes	1150:1251	the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes	1150:1251	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	7	76	dep	Cr	1532:1533	arg1	ions					1540:1543	ions	1540:1543	Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane	1532:1582	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	7	76	dep	Cr	1532:1533	arg1	VI					1536:1537	VI	1536:1537	VI	1536:1537	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	7	77	theme	CA/Ti-TEPA	1564:1573	arg1	membrane					1575:1582	CA/Ti-TEPA membrane	1564:1582	CA/Ti-TEPA membrane	1564:1582	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	0	78	theme	cellulose	105:113	arg1	membranes					123:131	modified cellulose acetate membranes	96:131	modified cellulose acetate membranes	96:131	Removal of chromium (VI) ions from aqueous solutions using amine-impregnated TiO2 nanoparticles modified cellulose acetate membranes.
29078191	7	79	theme	3.5	1554:1556	arg1	pH					1548:1549	pH	1548:1549	pH of 3.5	1548:1556	The maximum removal efficiency result of Cr (VI) ions at pH of 3.5 using CA/Ti-TEPA membrane was 99.8%.
29078191	2	80	theme	aqueous	490:496	arg1	solution					498:505	aqueous solution	490:505	aqueous solution	490:505	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	6	81	theme	amine	1320:1324	arg1	composite					1340:1348	amine modified TiO2 composite	1320:1348	amine modified TiO2 composite	1320:1348	Incorporation of amine modified TiO2 composite to the CA polymer was found to improve the fouling and removal characteristics of the membranes during the chromium ultrafiltration process.
29078191	8	82	theme	prospect	1683:1690	arg1	applications					1703:1714	prospect industrial applications	1683:1714	prospect industrial applications of the prepared membranes	1683:1740	The washing/regeneration cycle results in this study described as an essential part for prospect industrial applications of the prepared membranes.
29078191	5	83	theme	pure	1088:1091	arg1	dependent					1258:1266	dependent	1258:1266	dependent	1258:1266	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	5	83	theme	pure	1088:1091	arg1	permeability					1099:1110	The pure water permeability	1084:1110	The pure water permeability	1084:1110	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	4	84	dep	Fourier	686:692	arg1	transform					694:702	transform	694:702	transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies	694:921	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	2	85	theme	ultrafiltration	399:413	arg1	membranes					415:423	ultrafiltration membranes	399:423	ultrafiltration membranes with enhanced capacity	399:446	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	5	86	theme	modified	1194:1201	arg1	membranes					1243:1251	the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes	1150:1251	the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes	1150:1251	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	4	87	theme	gravimetric	761:771	arg1	analysis					773:780	thermo gravimetric analysis	754:780	thermo gravimetric analysis (TGA)	754:786	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	4	87	theme	gravimetric	761:771	arg1	TGA					783:785	TGA	783:785	TGA	783:785	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	9	88	dep	Cr	1755:1756	arg1	VI					1759:1760	VI	1759:1760	VI	1759:1760	The maximum Cr (VI) removal results by using CA/Ti-TEPA membrane for four washing/regeneration cycles are 99.6%, 99.5%, 98.6% and, 96.6%, respectively.
29078191	9	88	dep	Cr	1755:1756	arg1	results					1771:1777	removal results	1763:1777	The maximum Cr (VI) removal results by using CA/Ti-TEPA membrane for four washing/regeneration cycles	1743:1843	The maximum Cr (VI) removal results by using CA/Ti-TEPA membrane for four washing/regeneration cycles are 99.6%, 99.5%, 98.6% and, 96.6%, respectively.
29078191	4	89	theme	structure	984:992	arg1	terms					966:970	terms	966:970	terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance	966:1081	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	1	90	dep	tetraethylenepentamine	193:214	arg1	amines					281:286	amines	281:286	amines using impregnation process	281:313	In this work, TiO2 nanoparticles (NPs) were modified using tetraethylenepentamine (TEPA), ethylenediamine (EDA), and hexamethylenetetramine (HMTA) amines using impregnation process.
29078191	2	91	theme	modified	335:342	arg1	additive					377:384	additive	377:384	additive	377:384	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	2	91	theme	modified	335:342	arg1	samples					349:355	The prepared amine modified TiO2 samples	316:355	The prepared amine modified TiO2 samples	316:355	The prepared amine modified TiO2 samples were explored as an additive to fabricate ultrafiltration membranes with enhanced capacity towards the removal of chromium ions from aqueous solution.
29078191	6	92	theme	CA	1357:1358	arg1	polymer					1360:1366	the CA polymer	1353:1366	the CA polymer	1353:1366	Incorporation of amine modified TiO2 composite to the CA polymer was found to improve the fouling and removal characteristics of the membranes during the chromium ultrafiltration process.
29078191	5	93	theme	ion	1124:1126	arg1	efficiency					1136:1145	ion removal efficiency	1124:1145	Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes	1116:1251	The pure water permeability and Cr (VI) ion removal efficiency of the unmodified (i.e. CA/U-Ti) and the amine modified (CA/Ti-HMTA, CA/Ti-EDA, and CA/Ti-TEPA) membranes were dependent on pH and metal ion concentration.
29078191	4	94	theme	electric	995:1002	arg1	charge					1004:1009	electric charge	995:1009	electric charge	995:1009	Fourier transform infrared spectroscopy (FTIR), zeta potential (ζ), thermo gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), water contact angle (WCA), and atomic absorption spectrophotometer (AAS) studies were done to characterize the membranes in terms of chemical structure, electric charge, thermal stability, morphology, hydrophilicity, and removal performance.
29078191	3	95	theme	inversion	665:673	arg1	technique					675:683	phase inversion technique	659:683	phase inversion technique	659:683	Modified membranes were prepared from cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique.
29078191	3	96	theme	Modified	508:515	arg1	membranes					517:525	Modified membranes	508:525	Modified membranes	508:525	Modified membranes were prepared from cellulose acetate (CA) polymer blended with polyethylene glycol (PEG) additive, and amine modified TiO2 by using phase inversion technique.
24679144	5	0	theme	thermal	727:733	arg1	studies					745:751	the thermal stability studies	723:751	the thermal stability studies	723:751	At the thermal stability studies, the magnetic catalase beads protected 90% activity, while free catalase maintained only 10% activity at 70°C.
24679144	1	1	theme	study	150:154	arg1	enhancing					160:168	enhancing	160:168	enhancing of stability properties of catalase enzyme	160:211	The aim of this study was enhancing of stability properties of catalase enzyme by encapsulation in alginate/nanomagnetic beads.
24679144	1	1	theme	study	150:154	arg1	aim					138:140	The aim	134:140	The aim of this study	134:154	The aim of this study was enhancing of stability properties of catalase enzyme by encapsulation in alginate/nanomagnetic beads.
24679144	10	2	theme	alginate	1198:1205	arg1	beads					1207:1211	Catalase encapsulated magnetic alginate beads	1167:1211	Catalase encapsulated magnetic alginate beads	1167:1211	Catalase encapsulated magnetic alginate beads protected 83% activity after 50 cycles.
24679144	0	3	theme	composite	87:95	arg1	beads					97:101	alginate/Fe3O4 magnetic composite beads	63:101	alginate/Fe3O4 magnetic composite beads for enzymatic removal of H2O2	63:131	Improving of catalase stability properties by encapsulation in alginate/Fe3O4 magnetic composite beads for enzymatic removal of H2O2.
24679144	10	4	theme	encapsulated	1176:1187	arg1	beads					1207:1211	Catalase encapsulated magnetic alginate beads	1167:1211	Catalase encapsulated magnetic alginate beads	1167:1211	Catalase encapsulated magnetic alginate beads protected 83% activity after 50 cycles.
24679144	10	5	theme	magnetic	1189:1196	arg1	beads					1207:1211	Catalase encapsulated magnetic alginate beads	1167:1211	Catalase encapsulated magnetic alginate beads	1167:1211	Catalase encapsulated magnetic alginate beads protected 83% activity after 50 cycles.
24679144	0	6	theme	enzymatic	107:115	arg1	removal					117:123	enzymatic removal	107:123	enzymatic removal of H2O2	107:131	Improving of catalase stability properties by encapsulation in alginate/Fe3O4 magnetic composite beads for enzymatic removal of H2O2.
24679144	3	7	theme	operational	524:534	arg1	0-390 min					547:555	0-390 min	547:555	0-390 min	547:555	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	3	7	theme	operational	524:534	arg1	stability					536:544	pH stability (4.0-9.0) operational stability	501:544	pH stability (4.0-9.0) operational stability (0-390 min)	501:556	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	6	8	theme	large	929:933	arg1	area					935:938	a large area	927:938	a large area	927:938	The thermal profile of magnetic catalase beads was spread over a large area.
24679144	9	9	theme	activity	1116:1123	arg1	analysis					1125:1132	the activity analysis	1112:1132	the activity analysis	1112:1132	Thus, the activity analysis was done 50 times in succession.
24679144	2	10	theme	immobilization	362:375	arg1	conditions					377:386	immobilization conditions	362:386	immobilization conditions	362:386	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	4	11	theme	free	680:683	arg1	catalase					701:708	both free and immobilized catalase	675:708	both free and immobilized catalase	675:708	The optimum pH levels of both free and immobilized catalase were 7.0.
24679144	3	12	theme	kinetic	452:458	arg1	parameters					460:469	kinetic parameters	452:469	kinetic parameters	452:469	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	3	13	theme	pH	501:502	arg1	0-390 min					547:555	0-390 min	547:555	0-390 min	547:555	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	3	13	theme	pH	501:502	arg1	stability					536:544	pH stability (4.0-9.0) operational stability	501:544	pH stability (4.0-9.0) operational stability (0-390 min)	501:556	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	10	14	theme	%	1225:1225	arg1	activity					1227:1234	83% activity	1223:1234	83% activity	1223:1234	Catalase encapsulated magnetic alginate beads protected 83% activity after 50 cycles.
24679144	5	15	theme	free	812:815	arg1	catalase					817:824	free catalase	812:824	free catalase	812:824	At the thermal stability studies, the magnetic catalase beads protected 90% activity, while free catalase maintained only 10% activity at 70°C.
24679144	3	16	theme	stability	504:512	arg1	0-390 min					547:555	0-390 min	547:555	0-390 min	547:555	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	3	16	theme	stability	504:512	arg1	stability					536:544	pH stability (4.0-9.0) operational stability	501:544	pH stability (4.0-9.0) operational stability (0-390 min)	501:556	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	10	17	theme	83	1223:1224	arg1	%					1225:1225	%	1225:1225	%	1225:1225	Catalase encapsulated magnetic alginate beads protected 83% activity after 50 cycles.
24679144	5	18	theme	catalase	767:774	arg1	beads					776:780	the magnetic catalase beads	754:780	the magnetic catalase beads	754:780	At the thermal stability studies, the magnetic catalase beads protected 90% activity, while free catalase maintained only 10% activity at 70°C.
24679144	5	19	theme	stability	735:743	arg1	studies					745:751	the thermal stability studies	723:751	the thermal stability studies	723:751	At the thermal stability studies, the magnetic catalase beads protected 90% activity, while free catalase maintained only 10% activity at 70°C.
24679144	5	20	dep	activity	846:853	arg1	%					844:844	%	844:844	%	844:844	At the thermal stability studies, the magnetic catalase beads protected 90% activity, while free catalase maintained only 10% activity at 70°C.
24679144	1	21	theme	stability	173:181	arg1	properties					183:192	stability properties	173:192	stability properties of catalase enzyme	173:211	The aim of this study was enhancing of stability properties of catalase enzyme by encapsulation in alginate/nanomagnetic beads.
24679144	3	22	theme	optimum	399:405	arg1	25-50°C					420:426	25-50°C	420:426	25-50°C	420:426	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	3	22	theme	optimum	399:405	arg1	temperature					407:417	the optimum temperature	395:417	the optimum temperature (25-50°C)	395:427	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	4	23	theme	catalase	701:708	arg1	levels					665:670	The optimum pH levels	650:670	The optimum pH levels of both free and immobilized catalase	650:708	The optimum pH levels of both free and immobilized catalase were 7.0.
24679144	4	23	theme	catalase	701:708	arg1	7.0					715:717	7.0	715:717	7.0	715:717	The optimum pH levels of both free and immobilized catalase were 7.0.
24679144	1	24	theme	properties	183:192	arg1	enhancing					160:168	enhancing	160:168	enhancing of stability properties of catalase enzyme	160:211	The aim of this study was enhancing of stability properties of catalase enzyme by encapsulation in alginate/nanomagnetic beads.
24679144	1	24	theme	properties	183:192	arg1	aim					138:140	The aim	134:140	The aim of this study	134:154	The aim of this study was enhancing of stability properties of catalase enzyme by encapsulation in alginate/nanomagnetic beads.
24679144	0	25	theme	stability	22:30	arg1	properties					32:41	catalase stability properties	13:41	catalase stability properties	13:41	Improving of catalase stability properties by encapsulation in alginate/Fe3O4 magnetic composite beads for enzymatic removal of H2O2.
24679144	5	26	dep	%	844:844	arg1	10					842:843	10	842:843	10	842:843	At the thermal stability studies, the magnetic catalase beads protected 90% activity, while free catalase maintained only 10% activity at 70°C.
24679144	2	27	theme	concentrations	304:317	arg1	Amounts					262:268	Amounts	262:268	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL)	262:334	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	2	27	theme	concentrations	304:317	arg1	0.25-1.5 mg/mL					320:333	0.25-1.5 mg/mL	320:333	0.25-1.5 mg/mL	320:333	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	2	27	theme	concentrations	304:317	arg1	concentrations					304:317	enzyme concentrations	297:317	enzyme concentrations (0.25-1.5 mg/mL)	297:334	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	2	27	theme	concentrations	304:317	arg1	10-100 mg					282:290	10-100 mg	282:290	10-100 mg	282:290	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	2	27	theme	concentrations	304:317	arg1	carrier					273:279	carrier	273:279	carrier (10-100 mg)	273:291	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	5	28	theme	%	794:794	arg1	activity					796:803	90% activity	792:803	90% activity	792:803	At the thermal stability studies, the magnetic catalase beads protected 90% activity, while free catalase maintained only 10% activity at 70°C.
24679144	0	29	theme	catalase	13:20	arg1	properties					32:41	catalase stability properties	13:41	catalase stability properties	13:41	Improving of catalase stability properties by encapsulation in alginate/Fe3O4 magnetic composite beads for enzymatic removal of H2O2.
24679144	0	30	theme	H2O2	128:131	arg1	removal					117:123	enzymatic removal	107:123	enzymatic removal of H2O2	107:131	Improving of catalase stability properties by encapsulation in alginate/Fe3O4 magnetic composite beads for enzymatic removal of H2O2.
24679144	2	31	theme	enzyme	297:302	arg1	concentrations					304:317	enzyme concentrations	297:317	enzyme concentrations (0.25-1.5 mg/mL)	297:334	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	2	31	theme	enzyme	297:302	arg1	0.25-1.5 mg/mL					320:333	0.25-1.5 mg/mL	320:333	0.25-1.5 mg/mL	320:333	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	1	32	theme	catalase	197:204	arg1	enzyme					206:211	catalase enzyme	197:211	catalase enzyme	197:211	The aim of this study was enhancing of stability properties of catalase enzyme by encapsulation in alginate/nanomagnetic beads.
24679144	6	33	theme	beads	905:909	arg1	profile					876:882	The thermal profile	864:882	The thermal profile of magnetic catalase beads	864:909	The thermal profile of magnetic catalase beads was spread over a large area.
24679144	3	34	theme	immobilized	621:631	arg1	system					642:647	the immobilized catalase system	617:647	the immobilized catalase system	617:647	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	3	35	theme	optimum	430:436	arg1	pH					438:439	optimum pH	430:439	optimum pH (3.0-8.0)	430:449	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	3	35	theme	optimum	430:436	arg1	3.0-8.0					442:448	3.0-8.0	442:448	3.0-8.0	442:448	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	1	36	theme	enzyme	206:211	arg1	properties					183:192	stability properties	173:192	stability properties of catalase enzyme	173:211	The aim of this study was enhancing of stability properties of catalase enzyme by encapsulation in alginate/nanomagnetic beads.
24679144	6	37	theme	catalase	896:903	arg1	beads					905:909	magnetic catalase beads	887:909	magnetic catalase beads	887:909	The thermal profile of magnetic catalase beads was spread over a large area.
24679144	7	38	theme	pH	995:996	arg1	stability					998:1006	the pH stability	991:1006	the pH stability	991:1006	Similarly, this system indicated the improving of the pH stability.
24679144	6	39	theme	magnetic	887:894	arg1	beads					905:909	magnetic catalase beads	887:909	magnetic catalase beads	887:909	The thermal profile of magnetic catalase beads was spread over a large area.
24679144	8	40	theme	industrial	1060:1069	arg1	applications					1071:1082	industrial applications	1060:1082	industrial applications	1060:1082	The reusability, which is especially important for industrial applications, was also determined.
24679144	3	41	theme	thermal	472:478	arg1	20-70°C					491:497	20-70°C	491:497	20-70°C	491:497	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	3	41	theme	thermal	472:478	arg1	stability					480:488	thermal stability	472:488	thermal stability (20-70°C)	472:498	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	5	42	theme	magnetic	758:765	arg1	beads					776:780	the magnetic catalase beads	754:780	the magnetic catalase beads	754:780	At the thermal stability studies, the magnetic catalase beads protected 90% activity, while free catalase maintained only 10% activity at 70°C.
24679144	10	43	theme	Catalase	1167:1174	arg1	beads					1207:1211	Catalase encapsulated magnetic alginate beads	1167:1211	Catalase encapsulated magnetic alginate beads	1167:1211	Catalase encapsulated magnetic alginate beads protected 83% activity after 50 cycles.
24679144	1	44	from	encapsulation	216:228	arg1	beads					255:259	alginate/nanomagnetic beads	233:259	alginate/nanomagnetic beads	233:259	The aim of this study was enhancing of stability properties of catalase enzyme by encapsulation in alginate/nanomagnetic beads.
24679144	2	45	theme	carrier	273:279	arg1	Amounts					262:268	Amounts	262:268	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL)	262:334	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	2	45	theme	carrier	273:279	arg1	0.25-1.5 mg/mL					320:333	0.25-1.5 mg/mL	320:333	0.25-1.5 mg/mL	320:333	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	2	45	theme	carrier	273:279	arg1	concentrations					304:317	enzyme concentrations	297:317	enzyme concentrations (0.25-1.5 mg/mL)	297:334	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	2	45	theme	carrier	273:279	arg1	10-100 mg					282:290	10-100 mg	282:290	10-100 mg	282:290	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	2	45	theme	carrier	273:279	arg1	carrier					273:279	carrier	273:279	carrier (10-100 mg)	273:291	Amounts of carrier (10-100 mg) and enzyme concentrations (0.25-1.5 mg/mL) were analyzed to optimize immobilization conditions.
24679144	4	46	theme	immobilized	689:699	arg1	catalase					701:708	both free and immobilized catalase	675:708	both free and immobilized catalase	675:708	The optimum pH levels of both free and immobilized catalase were 7.0.
24679144	3	47	theme	catalase	633:640	arg1	system					642:647	the immobilized catalase system	617:647	the immobilized catalase system	617:647	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	0	48	theme	magnetic	78:85	arg1	beads					97:101	alginate/Fe3O4 magnetic composite beads	63:101	alginate/Fe3O4 magnetic composite beads for enzymatic removal of H2O2	63:131	Improving of catalase stability properties by encapsulation in alginate/Fe3O4 magnetic composite beads for enzymatic removal of H2O2.
24679144	3	49	theme	system	642:647	arg1	characterization					597:612	characterization	597:612	characterization of the immobilized catalase system	597:647	Also, the optimum temperature (25-50°C), optimum pH (3.0-8.0), kinetic parameters, thermal stability (20-70°C), pH stability (4.0-9.0) operational stability (0-390 min), and reusability were investigated for characterization of the immobilized catalase system.
24679144	4	50	theme	pH	662:663	arg1	levels					665:670	The optimum pH levels	650:670	The optimum pH levels of both free and immobilized catalase	650:708	The optimum pH levels of both free and immobilized catalase were 7.0.
24679144	4	50	theme	pH	662:663	arg1	7.0					715:717	7.0	715:717	7.0	715:717	The optimum pH levels of both free and immobilized catalase were 7.0.
24679144	1	51	theme	alginate/nanomagnetic	233:253	arg1	beads					255:259	alginate/nanomagnetic beads	233:259	alginate/nanomagnetic beads	233:259	The aim of this study was enhancing of stability properties of catalase enzyme by encapsulation in alginate/nanomagnetic beads.
24679144	0	52	theme	alginate/Fe3O4	63:76	arg1	beads					97:101	alginate/Fe3O4 magnetic composite beads	63:101	alginate/Fe3O4 magnetic composite beads for enzymatic removal of H2O2	63:131	Improving of catalase stability properties by encapsulation in alginate/Fe3O4 magnetic composite beads for enzymatic removal of H2O2.
24679144	6	53	theme	thermal	868:874	arg1	profile					876:882	The thermal profile	864:882	The thermal profile of magnetic catalase beads	864:909	The thermal profile of magnetic catalase beads was spread over a large area.
24679144	5	54	theme	90	792:793	arg1	%					794:794	%	794:794	%	794:794	At the thermal stability studies, the magnetic catalase beads protected 90% activity, while free catalase maintained only 10% activity at 70°C.
24679144	4	55	theme	optimum	654:660	arg1	levels					665:670	The optimum pH levels	650:670	The optimum pH levels of both free and immobilized catalase	650:708	The optimum pH levels of both free and immobilized catalase were 7.0.
24679144	4	55	theme	optimum	654:660	arg1	7.0					715:717	7.0	715:717	7.0	715:717	The optimum pH levels of both free and immobilized catalase were 7.0.
24619574	0	0	theme	implant	111:117	arg1	applications					119:130	dental implant applications	104:130	dental implant applications	104:130	Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications.
24619574	3	1	theme	single-phase	718:729	arg1	films					748:752	single-phase, pure HA-heparin films	718:752	single-phase, pure HA-heparin films of thickness 1/3 μm	718:772	In this study, we have developed an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm.
24619574	6	2	theme	coatings	1004:1011	arg1	bioactivities					965:977	The bioactivities	961:977	The bioactivities of the HA and HA-heparin coatings on Ti	961:1017	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	2	3	theme	coagulation	513:523	arg1	control					525:531	e.g. coagulation control	508:531	e.g. coagulation control	508:531	HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option.
24619574	3	4	theme	growth	697:702	arg1	procedure					704:712	an improved, simple and robust growth procedure	666:712	an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm	666:772	In this study, we have developed an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm.
24619574	6	5	theme	HA	986:987	arg1	bioactivities					965:977	The bioactivities	961:977	The bioactivities of the HA and HA-heparin coatings on Ti	961:1017	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	1	6	theme	hydroxyapatite	186:199	arg1	composites					214:223	hydroxyapatite (HA)-heparin composites	186:223	hydroxyapatite (HA)-heparin composites	186:223	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	0	7	from	Electrodeposition	0:16	arg1	titanium					91:98	titanium	91:98	titanium for dental implant applications	91:130	Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications.
24619574	3	8	theme	HA-heparin	737:746	arg1	films					748:752	single-phase, pure HA-heparin films	718:752	single-phase, pure HA-heparin films of thickness 1/3 μm	718:772	In this study, we have developed an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm.
24619574	2	9	theme	desirable	615:623	arg1	option					625:630	a highly desirable option	606:630	a highly desirable option	606:630	HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option.
24619574	5	10	theme	Staining	856:863	arg1	assays					865:870	Staining assays	856:870	Staining assays	856:870	Staining assays revealed homogeneous incorporation of sizable amounts of heparin in the composite films.
24619574	6	11	theme	cell	1041:1044	arg1	tests					1070:1074	HeLa cell proliferation/viability tests	1036:1074	HeLa cell proliferation/viability tests	1036:1074	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	2	12	theme	key	541:543	arg1	role					545:548	a key role	539:548	a key role	539:548	HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option.
24619574	0	13	theme	composite	69:77	arg1	coatings					79:86	nanostructured bioactive hydroxyapatite-heparin composite coatings	21:86	nanostructured bioactive hydroxyapatite-heparin composite coatings	21:86	Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications.
24619574	6	14	theme	HeLa	1036:1039	arg1	tests					1070:1074	HeLa cell proliferation/viability tests	1036:1074	HeLa cell proliferation/viability tests	1036:1074	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	5	15	theme	homogeneous	881:891	arg1	incorporation					893:905	homogeneous incorporation	881:905	homogeneous incorporation of sizable amounts of heparin in the composite films	881:958	Staining assays revealed homogeneous incorporation of sizable amounts of heparin in the composite films.
24619574	0	16	theme	dental	104:109	arg1	applications					119:130	dental implant applications	104:130	dental implant applications	104:130	Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications.
24619574	6	17	theme	HA-heparin	993:1002	arg1	coatings					1004:1011	HA-heparin coatings	993:1011	HA-heparin coatings	993:1011	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	2	18	theme	osteointegration	406:421	arg1	enhancers					423:431	osteointegration enhancers	406:431	osteointegration enhancers	406:431	HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option.
24619574	1	19	theme	structure	304:312	arg1	terms					295:299	terms	295:299	terms of structure, morphology, heparin content and bioactivity	295:357	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	6	20	from	Ti	1016:1017	arg1	bioactivities					965:977	The bioactivities	961:977	The bioactivities of the HA and HA-heparin coatings on Ti	961:1017	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	1	21	theme	-heparin	205:212	arg1	composites					214:223	hydroxyapatite (HA)-heparin composites	186:223	hydroxyapatite (HA)-heparin composites	186:223	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	0	22	theme	bioactive	36:44	arg1	coatings					79:86	nanostructured bioactive hydroxyapatite-heparin composite coatings	21:86	nanostructured bioactive hydroxyapatite-heparin composite coatings	21:86	Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications.
24619574	3	23	theme	μm	771:772	arg1	films					748:752	single-phase, pure HA-heparin films	718:752	single-phase, pure HA-heparin films of thickness 1/3 μm	718:772	In this study, we have developed an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm.
24619574	2	24	theme	anticoagulant	578:590	arg1	incorporation					558:570	the incorporation	554:570	the incorporation of an anticoagulant	554:590	HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option.
24619574	1	25	theme	composites	214:223	arg1	fabrication					171:181	the one-pot fabrication	159:181	the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity	159:357	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	0	26	theme	nanostructured	21:34	arg1	coatings					79:86	nanostructured bioactive hydroxyapatite-heparin composite coatings	21:86	nanostructured bioactive hydroxyapatite-heparin composite coatings	21:86	Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications.
24619574	1	27	theme	morphology	315:324	arg1	terms					295:299	terms	295:299	terms of structure, morphology, heparin content and bioactivity	295:357	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	5	28	theme	heparin	929:935	arg1	heparin					929:935	heparin	929:935	heparin	929:935	Staining assays revealed homogeneous incorporation of sizable amounts of heparin in the composite films.
24619574	5	28	theme	heparin	929:935	arg1	amounts					918:924	sizable amounts	910:924	sizable amounts of heparin	910:935	Staining assays revealed homogeneous incorporation of sizable amounts of heparin in the composite films.
24619574	5	29	theme	sizable	910:916	arg1	heparin					929:935	heparin	929:935	heparin	929:935	Staining assays revealed homogeneous incorporation of sizable amounts of heparin in the composite films.
24619574	5	29	theme	sizable	910:916	arg1	amounts					918:924	sizable amounts	910:924	sizable amounts of heparin	910:935	Staining assays revealed homogeneous incorporation of sizable amounts of heparin in the composite films.
24619574	2	30	theme	healing	451:457	arg1	rate					459:462	post-implant healing rate	438:462	post-implant healing rate in dental applications	438:485	HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option.
24619574	2	31	theme	e.g.	508:511	arg1	control					525:531	e.g. coagulation control	508:531	e.g. coagulation control	508:531	HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option.
24619574	3	32	theme	thickness	757:765	arg1	μm					771:772	thickness 1/3 μm	757:772	thickness 1/3 μm	757:772	In this study, we have developed an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm.
24619574	1	33	from	characterisation	275:290	arg1	terms					295:299	terms	295:299	terms of structure, morphology, heparin content and bioactivity	295:357	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	3	34	theme	improved	669:676	arg1	procedure					704:712	an improved, simple and robust growth procedure	666:712	an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm	666:772	In this study, we have developed an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm.
24619574	2	35	theme	post-implant	438:449	arg1	rate					459:462	post-implant healing rate	438:462	post-implant healing rate in dental applications	438:485	HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option.
24619574	0	36	theme	hydroxyapatite-heparin	46:67	arg1	coatings					79:86	nanostructured bioactive hydroxyapatite-heparin composite coatings	21:86	nanostructured bioactive hydroxyapatite-heparin composite coatings	21:86	Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications.
24619574	1	37	theme	heparin	327:333	arg1	content					335:341	heparin content	327:341	heparin content	327:341	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	2	38	theme	dental	467:472	arg1	applications					474:485	dental applications	467:485	dental applications	467:485	HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option.
24619574	1	39	from	electrodeposition	228:244	arg1	terms					295:299	terms	295:299	terms of structure, morphology, heparin content and bioactivity	295:357	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	6	40	theme	proliferation/viability	1046:1068	arg1	tests					1070:1074	HeLa cell proliferation/viability tests	1036:1074	HeLa cell proliferation/viability tests	1036:1074	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	1	41	theme	content	335:341	arg1	terms					295:299	terms	295:299	terms of structure, morphology, heparin content and bioactivity	295:357	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	4	42	theme	bone	835:838	arg1	mineralisation					840:853	bone mineralisation	835:853	bone mineralisation	835:853	HA-heparin, forming nanowires, has the ideal morphology for bone mineralisation.
24619574	0	43	theme	coatings	79:86	arg1	Electrodeposition					0:16	Electrodeposition	0:16	Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications.	0:131	Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications.
24619574	6	44	from	HA	986:987	arg1	Ti					1016:1017	Ti	1016:1017	Ti	1016:1017	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	6	45	dep	HA	986:987	arg1	the					982:984	the	982:984	the	982:984	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	6	46	from	coatings	1004:1011	arg1	Ti					1016:1017	Ti	1016:1017	Ti	1016:1017	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	1	47	theme	bioactivity	347:357	arg1	terms					295:299	terms	295:299	terms of structure, morphology, heparin content and bioactivity	295:357	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	3	48	dep	single-phase	718:729	arg1	pure					732:735	pure	732:735	pure	732:735	In this study, we have developed an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm.
24619574	4	49	theme	ideal	814:818	arg1	morphology					820:829	the ideal morphology	810:829	the ideal morphology for bone mineralisation	810:853	HA-heparin, forming nanowires, has the ideal morphology for bone mineralisation.
24619574	2	50	theme	HA	360:361	arg1	coatings					363:370	HA coatings	360:370	HA coatings	360:370	HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option.
24619574	6	51	theme	anticoagulant	1124:1136	arg1	presence					1108:1115	the presence	1104:1115	the presence of the anticoagulant	1104:1136	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	1	52	theme	Ti	251:252	arg1	substrates					254:263	Ti substrates	251:263	Ti substrates	251:263	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	6	53	from	bioactivities	965:977	arg1	Ti					1016:1017	Ti	1016:1017	Ti	1016:1017	The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.
24619574	5	54	from	incorporation	893:905	arg1	films					954:958	the composite films	940:958	the composite films	940:958	Staining assays revealed homogeneous incorporation of sizable amounts of heparin in the composite films.
24619574	5	55	theme	amounts	918:924	arg1	incorporation					893:905	homogeneous incorporation	881:905	homogeneous incorporation of sizable amounts of heparin in the composite films	881:958	Staining assays revealed homogeneous incorporation of sizable amounts of heparin in the composite films.
24619574	5	56	theme	composite	944:952	arg1	films					954:958	the composite films	940:958	the composite films	940:958	Staining assays revealed homogeneous incorporation of sizable amounts of heparin in the composite films.
24619574	1	57	theme	one-pot	163:169	arg1	fabrication					171:181	the one-pot fabrication	159:181	the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity	159:357	In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity.
24619574	2	58	from	rate	459:462	arg1	applications					474:485	dental applications	467:485	dental applications	467:485	HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option.
24619574	4	59	contain	has	806:808	arg1	HA-heparin					775:784	HA-heparin	775:784	HA-heparin	775:784	HA-heparin, forming nanowires, has the ideal morphology for bone mineralisation.
24619574	4	59	contain	has	806:808	arg2	morphology					820:829	the ideal morphology	810:829	the ideal morphology for bone mineralisation	810:853	HA-heparin, forming nanowires, has the ideal morphology for bone mineralisation.
24619574	3	60	dep	improved	669:676	arg1	simple					679:684	simple	679:684	simple	679:684	In this study, we have developed an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm.
24619574	3	60	dep	improved	669:676	arg1	robust					690:695	robust	690:695	robust	690:695	In this study, we have developed an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm.
26478386	6	0	theme	%	1080:1080	arg1	decrease					1055:1062	a decrease	1053:1062	a decrease of gentamicin EE% that can be attributed to the high volume of water used to remove PVA and NaCl residues	1053:1168	Process scale up resulted in a decrease of gentamicin EE% that can be attributed to the high volume of water used to remove PVA and NaCl residues.
26478386	4	1	theme	Experiment	639:648	arg1	design					666:671	Experiment (DOE) screening design	639:671	Experiment (DOE) screening design	639:671	A Design Of Experiment (DOE) screening design was applied as an approach to scale up manufacturing step.
26478386	7	2	theme	microscopy	1447:1456	arg1	analysis					1458:1465	scanning electron microscopy analysis	1429:1465	scanning electron microscopy analysis	1429:1465	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	6	3	theme	PVA	1148:1150	arg1	residues					1161:1168	PVA and NaCl residues	1148:1168	PVA and NaCl residues	1148:1168	Process scale up resulted in a decrease of gentamicin EE% that can be attributed to the high volume of water used to remove PVA and NaCl residues.
26478386	9	4	theme	high	1901:1904	arg1	potential					1906:1914	the high potential	1897:1914	the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1897:2012	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	4	5	theme	DOE	651:653	arg1	design					666:671	Experiment (DOE) screening design	639:671	Experiment (DOE) screening design	639:671	A Design Of Experiment (DOE) screening design was applied as an approach to scale up manufacturing step.
26478386	8	6	theme	microbiologic	1684:1696	arg1	evaluation					1698:1707	the microbiologic evaluation	1680:1707	the microbiologic evaluation	1680:1707	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	7	7	theme	scanning	1429:1436	arg1	microscopy					1447:1456	scanning electron microscopy	1429:1456	scanning electron microscopy analysis	1429:1465	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	1	8	theme	DOE	194:196	arg1	application					198:208	the DOE application	190:208	the DOE application to the preparation process of biodegradable microspheres for osteomyelitis local therapy	190:297	The present paper takes into account the DOE application to the preparation process of biodegradable microspheres for osteomyelitis local therapy.
26478386	6	9	theme	water	1127:1131	arg1	volume					1117:1122	the high volume	1108:1122	the high volume of water used to remove PVA and NaCl residues	1108:1168	Process scale up resulted in a decrease of gentamicin EE% that can be attributed to the high volume of water used to remove PVA and NaCl residues.
26478386	9	10	theme	microparticulate	1923:1938	arg1	system					1954:1959	the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1919:2012	the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1919:2012	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	9	11	theme	delivery	1945:1952	arg1	system					1954:1959	the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1919:2012	the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1919:2012	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	5	12	theme	DOE	747:749	arg1	design					761:766	DOE screening design	747:766	DOE screening design	747:766	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	6	13	theme	EE	1078:1079	arg1	%					1080:1080	gentamicin EE%	1067:1080	gentamicin EE%	1067:1080	Process scale up resulted in a decrease of gentamicin EE% that can be attributed to the high volume of water used to remove PVA and NaCl residues.
26478386	1	14	theme	preparation	217:227	arg1	process					229:235	the preparation process	213:235	the preparation process of biodegradable microspheres	213:265	The present paper takes into account the DOE application to the preparation process of biodegradable microspheres for osteomyelitis local therapy.
26478386	5	15	theme	design	761:766	arg1	results					736:742	The results	732:742	The results of DOE screening design	732:766	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	8	16	theme	loaded	1753:1758	arg1	microspheres					1760:1771	the gentamicin loaded microspheres	1738:1771	the gentamicin loaded microspheres	1738:1771	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	5	17	theme	EE	882:883	arg1	distribution					933:944	particle size distribution	919:944	particle size distribution	919:944	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	17	theme	EE	882:883	arg1	%					884:884	the EE%	878:884	the EE% (72.1-97.5%)	878:897	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	17	theme	EE	882:883	arg1	%					896:896	72.1-97.5%	887:896	72.1-97.5%	887:896	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	18	theme	size	928:931	arg1	distribution					933:944	particle size distribution	919:944	particle size distribution	919:944	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	6	19	theme	NaCl	1156:1159	arg1	residues					1161:1168	PVA and NaCl residues	1148:1168	PVA and NaCl residues	1148:1168	Process scale up resulted in a decrease of gentamicin EE% that can be attributed to the high volume of water used to remove PVA and NaCl residues.
26478386	9	20	theme	antibiotic	1986:1995	arg1	delivery					1997:2004	the local antibiotic delivery	1976:2004	the local antibiotic delivery to bone	1976:2012	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	7	21	theme	in	1186:1187	arg1	study					1214:1218	in vitro gentamicin release study	1186:1218	in vitro gentamicin release study	1186:1218	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	0	22	theme	design	16:21	arg1	approach					23:30	An experimental design approach	0:30	An experimental design approach to the preparation of pegylated polylactide-co-glicolide gentamicin loaded	0:105	An experimental design approach to the preparation of pegylated polylactide-co-glicolide gentamicin loaded microparticles for local antibiotic delivery.
26478386	8	23	theme	bovine	1636:1641	arg1	matrix					1648:1653	acellular bovine bone matrix	1626:1653	acellular bovine bone matrix (Orthoss® granules)	1626:1673	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	8	23	theme	bovine	1636:1641	arg1	granules					1665:1672	Orthoss® granules	1656:1672	Orthoss® granules	1656:1672	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	3	24	dep	protocols	451:459	arg1	o/w					462:464	o/w	462:464	o/w	462:464	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	3	24	dep	protocols	451:459	arg1	protocols					451:459	Two preparation protocols	435:459	Two preparation protocols (o/w and w/o/w)	435:475	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	3	24	dep	protocols	451:459	arg1	and					512:514	and	512:514	and	512:514	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	3	24	dep	protocols	451:459	arg1	PEG					609:611	PEG	609:611	PEG	609:611	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	3	24	dep	protocols	451:459	arg1	w/o/w					470:474	w/o/w	470:474	w/o/w	470:474	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	0	25	theme	gentamicin	89:98	arg1	preparation					39:49	the preparation	35:49	the preparation of pegylated polylactide-co-glicolide gentamicin loaded	35:105	An experimental design approach to the preparation of pegylated polylactide-co-glicolide gentamicin loaded microparticles for local antibiotic delivery.
26478386	5	26	theme	span	904:907	arg1	1.03-1.23					947:955	1.03-1.23	947:955	1.03-1.23	947:955	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	26	theme	span	904:907	arg1	values					909:914	span values	904:914	span values of particle size distribution (1.03-1.23)	904:956	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	1	27	theme	present	157:163	arg1	paper					165:169	The present paper	153:169	The present paper	153:169	The present paper takes into account the DOE application to the preparation process of biodegradable microspheres for osteomyelitis local therapy.
26478386	8	28	theme	gentamicin	1493:1502	arg1	release					1482:1488	The prolonged release	1468:1488	The prolonged release of gentamicin	1468:1502	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	0	29	theme	local	126:130	arg1	delivery					143:150	local antibiotic delivery	126:150	local antibiotic delivery	126:150	An experimental design approach to the preparation of pegylated polylactide-co-glicolide gentamicin loaded microparticles for local antibiotic delivery.
26478386	9	30	theme	collected	1798:1806	arg1	results					1808:1814	The collected results	1794:1814	The collected results	1794:1814	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	3	31	theme	acids	599:603	arg1	compositions					563:574	different compositions	553:574	different compositions of lactic and glycolic acids	553:603	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	3	32	theme	process	492:498	arg1	conditions					500:509	different process conditions	482:509	different process conditions	482:509	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	5	33	theme	w/v	832:834	arg1	concentration					844:856	the 15%w/v polymer concentration	825:856	the 15%w/v polymer concentration	825:856	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	3	34	theme	block	531:535	arg1	copolymers					537:546	three PLGA-PEG block copolymers	516:546	three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids	516:603	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	7	35	with	consistent	1367:1376	arg1	structure					1404:1412	their highly compact structure	1383:1412	their highly compact structure highlighted by scanning electron microscopy analysis	1383:1465	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	6	36	theme	scale	1032:1036	arg1	up					1038:1039	Process scale up	1024:1039	Process scale up	1024:1039	Process scale up resulted in a decrease of gentamicin EE% that can be attributed to the high volume of water used to remove PVA and NaCl residues.
26478386	0	37	theme	pegylated	54:62	arg1	gentamicin					89:98	pegylated polylactide-co-glicolide gentamicin	54:98	pegylated polylactide-co-glicolide gentamicin loaded	54:105	An experimental design approach to the preparation of pegylated polylactide-co-glicolide gentamicin loaded microparticles for local antibiotic delivery.
26478386	5	38	theme	15	829:830	arg1	%					831:831	%	831:831	%	831:831	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	3	39	dep	and	512:514	arg1	copolymers					537:546	three PLGA-PEG block copolymers	516:546	three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids	516:603	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	9	40	theme	up	1853:1854	arg1	feasibility					1828:1838	the feasibility	1824:1838	the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1824:2012	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	8	41	theme	bone	1643:1646	arg1	matrix					1648:1653	acellular bovine bone matrix	1626:1653	acellular bovine bone matrix (Orthoss® granules)	1626:1673	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	8	41	theme	bone	1643:1646	arg1	granules					1665:1672	Orthoss® granules	1656:1672	Orthoss® granules	1656:1672	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	1	42	theme	biodegradable	240:252	arg1	microspheres					254:265	biodegradable microspheres	240:265	biodegradable microspheres	240:265	The present paper takes into account the DOE application to the preparation process of biodegradable microspheres for osteomyelitis local therapy.
26478386	7	43	theme	prolonged	1225:1233	arg1	release					1246:1252	prolonged gentamicin release	1225:1252	prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase	1225:1345	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	9	44	theme	microsphere	1859:1869	arg1	process					1885:1891	microsphere manufacturing process	1859:1891	microsphere manufacturing process	1859:1891	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	8	45	theme	composite	1591:1599	arg1	gel					1601:1603	a thermosetting composite gel	1575:1603	a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules)	1575:1673	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	8	46	from	efficacy	1726:1733	arg1	coli					1788:1791	Escherichia coli	1776:1791	Escherichia coli	1776:1791	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	3	47	theme	glycolic	590:597	arg1	acids					599:603	lactic and glycolic acids	579:603	lactic and glycolic acids	579:603	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	7	48	dep	months	1266:1271	arg1	up					1254:1255	up	1254:1255	up	1254:1255	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	7	49	from	microspheres	1282:1293	arg1	release					1246:1252	prolonged gentamicin release	1225:1252	prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase	1225:1345	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	7	50	theme	study	1214:1218	arg1	results					1175:1181	The results	1171:1181	The results of in vitro gentamicin release study	1171:1218	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	9	51	theme	process	1885:1891	arg1	up					1853:1854	the scale up	1843:1854	the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1843:2012	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	4	52	theme	design	666:671	arg1	approach					691:698	an approach	688:698	an approach	688:698	A Design Of Experiment (DOE) screening design was applied as an approach to scale up manufacturing step.
26478386	4	52	theme	design	666:671	arg1	Design					629:634	A Design	627:634	A Design Of Experiment (DOE) screening design	627:671	A Design Of Experiment (DOE) screening design was applied as an approach to scale up manufacturing step.
26478386	3	53	theme	lactic	579:584	arg1	acids					599:603	lactic and glycolic acids	579:603	lactic and glycolic acids	579:603	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	1	54	theme	local	285:289	arg1	therapy					291:297	osteomyelitis local therapy	271:297	osteomyelitis local therapy	271:297	The present paper takes into account the DOE application to the preparation process of biodegradable microspheres for osteomyelitis local therapy.
26478386	7	55	theme	electron	1438:1445	arg1	microscopy					1447:1456	scanning electron microscopy	1429:1456	scanning electron microscopy analysis	1429:1465	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	7	56	theme	gentamicin	1195:1204	arg1	study					1214:1218	in vitro gentamicin release study	1186:1218	in vitro gentamicin release study	1186:1218	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	2	57	theme	loaded	326:331	arg1	microspheres					390:401	loaded polylactide-co-glycolide-copolyethyleneglycol (PLGA-PEG) microspheres	326:401	loaded polylactide-co-glycolide-copolyethyleneglycol (PLGA-PEG) microspheres	326:401	With this goal gentamicin loaded polylactide-co-glycolide-copolyethyleneglycol (PLGA-PEG) microspheres were prepared and investigated.
26478386	3	58	with	protocols	451:459	arg1	conditions					500:509	different process conditions	482:509	different process conditions	482:509	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	7	59	theme	compact	1396:1402	arg1	structure					1404:1412	their highly compact structure	1383:1412	their highly compact structure highlighted by scanning electron microscopy analysis	1383:1465	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	9	60	theme	potential	1906:1914	arg1	up					1853:1854	the scale up	1843:1854	the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1843:2012	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	8	61	theme	acellular	1626:1634	arg1	matrix					1648:1653	acellular bovine bone matrix	1626:1653	acellular bovine bone matrix (Orthoss® granules)	1626:1673	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	8	61	theme	acellular	1626:1634	arg1	granules					1665:1672	Orthoss® granules	1656:1672	Orthoss® granules	1656:1672	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	2	62	theme	goal	310:313	arg1	gentamicin					315:324	this goal gentamicin	305:324	this goal gentamicin	305:324	With this goal gentamicin loaded polylactide-co-glycolide-copolyethyleneglycol (PLGA-PEG) microspheres were prepared and investigated.
26478386	9	63	theme	scale	1847:1851	arg1	up					1853:1854	the scale up	1843:1854	the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1843:2012	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	9	64	theme	drug	1940:1943	arg1	system					1954:1959	the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1919:2012	the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1919:2012	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	6	65	theme	high	1112:1115	arg1	volume					1117:1122	the high volume	1108:1122	the high volume of water used to remove PVA and NaCl residues	1108:1168	Process scale up resulted in a decrease of gentamicin EE% that can be attributed to the high volume of water used to remove PVA and NaCl residues.
26478386	9	66	theme	system	1954:1959	arg1	potential					1906:1914	the high potential	1897:1914	the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone	1897:2012	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	9	66	theme	system	1954:1959	arg1	process					1885:1891	microsphere manufacturing process	1859:1891	microsphere manufacturing process	1859:1891	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	5	67	theme	screening	751:759	arg1	design					761:766	DOE screening design	747:766	DOE screening design	747:766	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	8	68	theme	gentamicin	1742:1751	arg1	microspheres					1760:1771	the gentamicin loaded microspheres	1738:1771	the gentamicin loaded microspheres	1738:1771	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	7	69	theme	dispersing	1330:1339	arg1	phase					1341:1345	the dispersing phase	1326:1345	the dispersing phase	1326:1345	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	8	70	theme	microspheres	1760:1771	arg1	efficacy					1726:1733	the efficacy	1722:1733	the efficacy of the gentamicin loaded microspheres on Escherichia coli	1722:1791	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	5	71	theme	particle	919:926	arg1	size					928:931	particle size	919:931	particle size distribution	919:944	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	6	72	theme	Process	1024:1030	arg1	up					1038:1039	Process scale up	1024:1039	Process scale up	1024:1039	Process scale up resulted in a decrease of gentamicin EE% that can be attributed to the high volume of water used to remove PVA and NaCl residues.
26478386	9	73	theme	local	1980:1984	arg1	delivery					1997:2004	the local antibiotic delivery	1976:2004	the local antibiotic delivery to bone	1976:2012	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	0	74	theme	polylactide-co-glicolide	64:87	arg1	gentamicin					89:98	pegylated polylactide-co-glicolide gentamicin	54:98	pegylated polylactide-co-glicolide gentamicin loaded	54:105	An experimental design approach to the preparation of pegylated polylactide-co-glicolide gentamicin loaded microparticles for local antibiotic delivery.
26478386	8	75	theme	prolonged	1472:1480	arg1	release					1482:1488	The prolonged release	1468:1488	The prolonged release of gentamicin	1468:1502	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	7	76	theme	salt	1309:1312	arg1	addition					1314:1321	salt addition	1309:1321	salt addition	1309:1321	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	2	77	theme	polylactide-co-glycolide-copolyethyleneglycol	333:377	arg1	microspheres					390:401	loaded polylactide-co-glycolide-copolyethyleneglycol (PLGA-PEG) microspheres	326:401	loaded polylactide-co-glycolide-copolyethyleneglycol (PLGA-PEG) microspheres	326:401	With this goal gentamicin loaded polylactide-co-glycolide-copolyethyleneglycol (PLGA-PEG) microspheres were prepared and investigated.
26478386	3	78	theme	preparation	439:449	arg1	o/w					462:464	o/w	462:464	o/w	462:464	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	3	78	theme	preparation	439:449	arg1	protocols					451:459	Two preparation protocols	435:459	Two preparation protocols (o/w and w/o/w)	435:475	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	3	78	theme	preparation	439:449	arg1	w/o/w					470:474	w/o/w	470:474	w/o/w	470:474	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	0	79	theme	antibiotic	132:141	arg1	delivery					143:150	local antibiotic delivery	126:150	local antibiotic delivery	126:150	An experimental design approach to the preparation of pegylated polylactide-co-glicolide gentamicin loaded microparticles for local antibiotic delivery.
26478386	5	80	theme	salt	965:968	arg1	addition					970:977	salt addition	965:977	salt addition alone	965:983	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	6	81	theme	gentamicin	1067:1076	arg1	%					1080:1080	gentamicin EE%	1067:1080	gentamicin EE%	1067:1080	Process scale up resulted in a decrease of gentamicin EE% that can be attributed to the high volume of water used to remove PVA and NaCl residues.
26478386	5	82	theme	w/o/w	783:787	arg1	technique					789:797	w/o/w technique	783:797	w/o/w technique	783:797	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	83	theme	%	831:831	arg1	concentration					844:856	the 15%w/v polymer concentration	825:856	the 15%w/v polymer concentration	825:856	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	84	theme	distribution	933:944	arg1	1.03-1.23					947:955	1.03-1.23	947:955	1.03-1.23	947:955	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	84	theme	distribution	933:944	arg1	distribution					933:944	particle size distribution	919:944	particle size distribution	919:944	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	84	theme	distribution	933:944	arg1	values					909:914	span values	904:914	span values of particle size distribution (1.03-1.23)	904:956	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	84	theme	distribution	933:944	arg1	%					884:884	the EE%	878:884	the EE% (72.1-97.5%)	878:897	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	84	theme	distribution	933:944	arg1	%					896:896	72.1-97.5%	887:896	72.1-97.5%	887:896	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	85	theme	polymer	836:842	arg1	concentration					844:856	the 15%w/v polymer concentration	825:856	the 15%w/v polymer concentration	825:856	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	8	86	theme	biodegradable	1543:1555	arg1	microspheres					1557:1568	the biodegradable microspheres	1539:1568	the biodegradable microspheres	1539:1568	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	5	87	theme	salt	816:819	arg1	technique					789:797	w/o/w technique	783:797	w/o/w technique	783:797	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	87	theme	salt	816:819	arg1	presence					804:811	the presence	800:811	the presence of salt	800:819	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	87	theme	salt	816:819	arg1	concentration					844:856	the 15%w/v polymer concentration	825:856	the 15%w/v polymer concentration	825:856	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	5	88	theme	yield	1009:1013	arg1	process					1015:1021	the yield process	1005:1021	the yield process	1005:1021	The results of DOE screening design confirmed that w/o/w technique, the presence of salt and the 15%w/v polymer concentration positively affected the EE% (72.1-97.5%), and span values of particle size distribution (1.03-1.23), while salt addition alone negatively affected the yield process.
26478386	3	89	theme	different	482:490	arg1	conditions					500:509	different process conditions	482:509	different process conditions	482:509	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	7	90	dep	in	1186:1187	arg1	vitro					1189:1193	vitro	1189:1193	vitro	1189:1193	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	3	91	theme	PLGA-PEG	522:529	arg1	copolymers					537:546	three PLGA-PEG block copolymers	516:546	three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids	516:603	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	7	92	theme	gentamicin	1235:1244	arg1	release					1246:1252	prolonged gentamicin release	1225:1252	prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase	1225:1345	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	3	93	with	copolymers	537:546	arg1	compositions					563:574	different compositions	553:574	different compositions of lactic and glycolic acids	553:603	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	8	94	theme	thermosetting	1577:1589	arg1	gel					1601:1603	a thermosetting composite gel	1575:1603	a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules)	1575:1673	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	4	95	theme	screening	656:664	arg1	design					666:671	Experiment (DOE) screening design	639:671	Experiment (DOE) screening design	639:671	A Design Of Experiment (DOE) screening design was applied as an approach to scale up manufacturing step.
26478386	3	96	theme	different	553:561	arg1	compositions					563:574	different compositions	553:574	different compositions of lactic and glycolic acids	553:603	Two preparation protocols (o/w and w/o/w) with different process conditions, and three PLGA-PEG block copolymers with different compositions of lactic and glycolic acids and PEG, were tested.
26478386	8	97	theme	Orthoss®	1656:1663	arg1	matrix					1648:1653	acellular bovine bone matrix	1626:1653	acellular bovine bone matrix (Orthoss® granules)	1626:1673	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	8	97	theme	Orthoss®	1656:1663	arg1	granules					1665:1672	Orthoss® granules	1656:1672	Orthoss® granules	1656:1672	The prolonged release of gentamicin is maintained even after embedding the biodegradable microspheres into a thermosetting composite gel made of chitosan and acellular bovine bone matrix (Orthoss® granules), and the microbiologic evaluation demonstrated the efficacy of the gentamicin loaded microspheres on Escherichia coli.
26478386	1	98	theme	microspheres	254:265	arg1	process					229:235	the preparation process	213:235	the preparation process of biodegradable microspheres	213:265	The present paper takes into account the DOE application to the preparation process of biodegradable microspheres for osteomyelitis local therapy.
26478386	7	99	theme	release	1206:1212	arg1	study					1214:1218	in vitro gentamicin release study	1186:1218	in vitro gentamicin release study	1186:1218	The results of in vitro gentamicin release study show prolonged gentamicin release up to three months from the microspheres prepared with salt addition in the dispersing phase; the behavior being consistent with their highly compact structure highlighted by scanning electron microscopy analysis.
26478386	9	100	theme	manufacturing	1871:1883	arg1	process					1885:1891	microsphere manufacturing process	1859:1891	microsphere manufacturing process	1859:1891	The collected results confirm the feasibility of the scale up of microsphere manufacturing process and the high potential of the microparticulate drug delivery system to be used for the local antibiotic delivery to bone.
26478386	0	101	theme	experimental	3:14	arg1	approach					23:30	An experimental design approach	0:30	An experimental design approach to the preparation of pegylated polylactide-co-glicolide gentamicin loaded	0:105	An experimental design approach to the preparation of pegylated polylactide-co-glicolide gentamicin loaded microparticles for local antibiotic delivery.
26478386	1	102	theme	osteomyelitis	271:283	arg1	therapy					291:297	osteomyelitis local therapy	271:297	osteomyelitis local therapy	271:297	The present paper takes into account the DOE application to the preparation process of biodegradable microspheres for osteomyelitis local therapy.
27988076	1	0	theme	amount	368:373	arg1	extraction					348:357	mat-based extraction	338:357	mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection	338:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	2	1	dep	Fourier	576:582	arg1	transform					584:592	transform	584:592	transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and N2 adsorption-desorption experiments	584:704	The composite nanofibres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and N2 adsorption-desorption experiments.
27988076	4	2	theme	expert	935:940	arg1	software					947:954	design expert (DX) software	928:954	design expert (DX) software	928:954	The optimum point was achieved by analysis of the results according to design expert (DX) software.
27988076	1	3	theme	high	467:470	arg1	detection					518:526	high performance liquid chromatography-ultraviolet detection	467:526	high performance liquid chromatography-ultraviolet detection	467:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	4	4	theme	DX	943:944	arg1	software					947:954	design expert (DX) software	928:954	design expert (DX) software	928:954	The optimum point was achieved by analysis of the results according to design expert (DX) software.
27988076	1	5	theme	cheap	279:283	arg1	method					315:320	a simple, cheap and accessible electrospining method	269:320	a simple, cheap and accessible electrospining method	269:320	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	1	6	theme	Δ9-tetrahydrocannabinol	378:400	arg1	THC					403:405	THC	403:405	THC	403:405	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	1	6	theme	Δ9-tetrahydrocannabinol	378:400	arg1	Δ9-tetrahydrocannabinol					378:400	Δ9-tetrahydrocannabinol	378:400	Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection	378:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	1	6	theme	Δ9-tetrahydrocannabinol	378:400	arg1	amount					368:373	trace amount	362:373	trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection	362:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	2	7	theme	adsorption-desorption	672:692	arg1	experiments					694:704	N2 adsorption-desorption experiments	669:704	N2 adsorption-desorption experiments	669:704	The composite nanofibres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and N2 adsorption-desorption experiments.
27988076	1	8	theme	performance	472:482	arg1	detection					518:526	high performance liquid chromatography-ultraviolet detection	467:526	high performance liquid chromatography-ultraviolet detection	467:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	8	9	theme	trace	1541:1545	arg1	determination					1547:1559	trace determination	1541:1559	trace determination of THC in the human whole blood samples	1541:1599	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	0	10	theme	Δ9-tetrahydrocannabinol	99:121	arg1	extraction					85:94	solid-phase extraction	73:94	solid-phase extraction of Δ9-tetrahydrocannabinol	73:121	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite nanofibers for solid-phase extraction of Δ9-tetrahydrocannabinol in whole blood samples using Box-Behnken experimental design.
27988076	2	11	theme	N2	669:670	arg1	experiments					694:704	N2 adsorption-desorption experiments	669:704	N2 adsorption-desorption experiments	669:704	The composite nanofibres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and N2 adsorption-desorption experiments.
27988076	1	12	theme	liquid	484:489	arg1	detection					518:526	high performance liquid chromatography-ultraviolet detection	467:526	high performance liquid chromatography-ultraviolet detection	467:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	5	13	theme	significant	1015:1025	arg1	volume					961:966	The volume	957:966	The volume of eluting solvent	957:985	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	5	13	theme	significant	1015:1025	arg1	variables					1027:1035	significant variables	1015:1035	significant variables	1015:1035	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	1	14	theme	accessible	289:298	arg1	method					315:320	a simple, cheap and accessible electrospining method	269:320	a simple, cheap and accessible electrospining method	269:320	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	0	15	theme	blood	132:136	arg1	samples					138:144	whole blood samples	126:144	whole blood samples using Box-Behnken experimental design	126:182	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite nanofibers for solid-phase extraction of Δ9-tetrahydrocannabinol in whole blood samples using Box-Behnken experimental design.
27988076	8	16	from	THC	1564:1566	arg1	samples					1593:1599	the human whole blood samples	1571:1599	the human whole blood samples	1571:1599	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	1	17	theme	chromatography-ultraviolet	491:516	arg1	detection					518:526	high performance liquid chromatography-ultraviolet detection	467:526	high performance liquid chromatography-ultraviolet detection	467:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	6	18	theme	0.1-100μgL-1	1203:1214	arg1	range					1194:1198	a linear range	1185:1198	a linear range of 0.1-100μgL-1 (R2=0.9943) for THC	1185:1234	Under the optimum conditions, the method was exhibited a linear range of 0.1-100μgL-1 (R2=0.9943) for THC with a detection limit of 0.04μgL-1.
27988076	0	19	theme	whole	126:130	arg1	samples					138:144	whole blood samples	126:144	whole blood samples using Box-Behnken experimental design	126:182	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite nanofibers for solid-phase extraction of Δ9-tetrahydrocannabinol in whole blood samples using Box-Behnken experimental design.
27988076	4	20	theme	results	907:913	arg1	analysis					891:898	analysis	891:898	analysis of the results according to design expert (DX) software	891:954	The optimum point was achieved by analysis of the results according to design expert (DX) software.
27988076	7	21	dep	intra-day	1295:1303	arg1	deviations					1351:1360	relative standard deviations	1333:1360	relative standard deviations	1333:1360	Acceptable values for intra-day (3.2%) and inter-day (4.8%) relative standard deviations were obtained.
27988076	1	22	theme	biocompatible	203:215	arg1	composite					239:247	biocompatible Chitosan/MIL-101 (Fe) composite	203:247	biocompatible Chitosan/MIL-101 (Fe) composite	203:247	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	0	23	theme	biocompatible	12:24	arg1	composite					48:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite	0:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite	0:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite nanofibers for solid-phase extraction of Δ9-tetrahydrocannabinol in whole blood samples using Box-Behnken experimental design.
27988076	8	24	theme	method	1530:1535	arg1	capability					1512:1521	the capability	1508:1521	the capability of the method for trace determination of THC in the human whole blood samples	1508:1599	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	4	25	theme	design	928:933	arg1	software					947:954	design expert (DX) software	928:954	design expert (DX) software	928:954	The optimum point was achieved by analysis of the results according to design expert (DX) software.
27988076	5	26	theme	sorbent	988:994	arg1	solvent					979:985	eluting solvent	971:985	eluting solvent	971:985	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	5	26	theme	sorbent	988:994	arg1	amount					996:1001	sorbent amount	988:1001	sorbent amount	988:1001	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	8	27	theme	human	1575:1579	arg1	samples					1593:1599	the human whole blood samples	1571:1599	the human whole blood samples	1571:1599	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	1	28	theme	Chitosan/MIL-101	217:232	arg1	composite					239:247	biocompatible Chitosan/MIL-101 (Fe) composite	203:247	biocompatible Chitosan/MIL-101 (Fe) composite	203:247	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	0	29	theme	Electrospun	0:10	arg1	composite					48:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite	0:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite	0:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite nanofibers for solid-phase extraction of Δ9-tetrahydrocannabinol in whole blood samples using Box-Behnken experimental design.
27988076	1	30	theme	electrospining	300:313	arg1	method					315:320	a simple, cheap and accessible electrospining method	269:320	a simple, cheap and accessible electrospining method	269:320	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	6	31	theme	0.04μgL-1	1262:1270	arg1	limit					1253:1257	a detection limit	1241:1257	a detection limit of 0.04μgL-1	1241:1270	Under the optimum conditions, the method was exhibited a linear range of 0.1-100μgL-1 (R2=0.9943) for THC with a detection limit of 0.04μgL-1.
27988076	8	32	theme	blood	1467:1471	arg1	samples					1473:1479	whole blood samples	1461:1479	whole blood samples	1461:1479	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	6	33	theme	linear	1187:1192	arg1	range					1194:1198	a linear range	1185:1198	a linear range of 0.1-100μgL-1 (R2=0.9943) for THC	1185:1234	Under the optimum conditions, the method was exhibited a linear range of 0.1-100μgL-1 (R2=0.9943) for THC with a detection limit of 0.04μgL-1.
27988076	8	34	from	recoveries	1433:1442	arg1	samples					1473:1479	whole blood samples	1461:1479	whole blood samples	1461:1479	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	3	35	theme	sorbent	738:744	arg1	amount					746:751	sorbent amount	738:751	sorbent amount	738:751	The volume of eluting solvent, sorbent amount, pH and% NaCl (w/v) influencing on the responses were investigated using factorial experimental design.
27988076	2	36	theme	scanning	617:624	arg1	microscopy					635:644	scanning electron microscopy	617:644	scanning electron microscopy	617:644	The composite nanofibres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and N2 adsorption-desorption experiments.
27988076	3	37	theme	factorial	826:834	arg1	design					849:854	factorial experimental design	826:854	factorial experimental design	826:854	The volume of eluting solvent, sorbent amount, pH and% NaCl (w/v) influencing on the responses were investigated using factorial experimental design.
27988076	2	38	dep	transform	584:592	arg1	infrared					594:601	infrared	594:601	transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and N2 adsorption-desorption experiments	584:704	The composite nanofibres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and N2 adsorption-desorption experiments.
27988076	8	39	theme	high	1381:1384	arg1	factor					1403:1408	The high preconcentration factor (970) and satisfactory recoveries	1377:1442	factor	1403:1408	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	8	39	theme	high	1381:1384	arg1	%					1455:1455	88.2%-92.4%	1445:1455	88.2%-92.4%	1445:1455	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	1	40	theme	Fe	235:236	arg1	composite					239:247	biocompatible Chitosan/MIL-101 (Fe) composite	203:247	biocompatible Chitosan/MIL-101 (Fe) composite	203:247	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	0	41	theme	Chitosan/MIL-101	26:41	arg1	composite					48:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite	0:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite	0:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite nanofibers for solid-phase extraction of Δ9-tetrahydrocannabinol in whole blood samples using Box-Behnken experimental design.
27988076	2	42	theme	electron	626:633	arg1	microscopy					635:644	scanning electron microscopy	617:644	scanning electron microscopy	617:644	The composite nanofibres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and N2 adsorption-desorption experiments.
27988076	1	43	theme	human	413:417	arg1	sample					431:436	human whole blood sample	413:436	human whole blood sample	413:436	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	2	44	theme	composite	533:541	arg1	nanofibres					543:552	The composite nanofibres	529:552	The composite nanofibres	529:552	The composite nanofibres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and N2 adsorption-desorption experiments.
27988076	8	45	theme	blood	1587:1591	arg1	samples					1593:1599	the human whole blood samples	1571:1599	the human whole blood samples	1571:1599	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	0	46	theme	experimental	164:175	arg1	design					177:182	Box-Behnken experimental design	152:182	Box-Behnken experimental design	152:182	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite nanofibers for solid-phase extraction of Δ9-tetrahydrocannabinol in whole blood samples using Box-Behnken experimental design.
27988076	1	47	theme	whole	419:423	arg1	sample					431:436	human whole blood sample	413:436	human whole blood sample	413:436	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	8	48	theme	THC	1564:1566	arg1	determination					1547:1559	trace determination	1541:1559	trace determination of THC in the human whole blood samples	1541:1599	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	1	49	theme	composite	239:247	arg1	nanofibers					189:198	The nanofibers	185:198	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite	185:247	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	0	50	theme	Fe	44:45	arg1	composite					48:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite	0:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite	0:56	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite nanofibers for solid-phase extraction of Δ9-tetrahydrocannabinol in whole blood samples using Box-Behnken experimental design.
27988076	0	51	theme	Box-Behnken	152:162	arg1	design					177:182	Box-Behnken experimental design	152:182	Box-Behnken experimental design	152:182	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite nanofibers for solid-phase extraction of Δ9-tetrahydrocannabinol in whole blood samples using Box-Behnken experimental design.
27988076	1	52	theme	blood	425:429	arg1	sample					431:436	human whole blood sample	413:436	human whole blood sample	413:436	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	8	53	theme	preconcentration	1386:1401	arg1	factor					1403:1408	The high preconcentration factor (970) and satisfactory recoveries	1377:1442	factor	1403:1408	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	8	53	theme	preconcentration	1386:1401	arg1	%					1455:1455	88.2%-92.4%	1445:1455	88.2%-92.4%	1445:1455	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	5	54	theme	best	1104:1107	arg1	response					1120:1127	the best extraction response	1100:1127	the best extraction response	1100:1127	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	1	55	theme	mat-based	338:346	arg1	extraction					348:357	mat-based extraction	338:357	mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection	338:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	8	56	theme	%	1449:1449	arg1	factor					1403:1408	The high preconcentration factor (970) and satisfactory recoveries	1377:1442	factor	1403:1408	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	8	56	theme	%	1449:1449	arg1	%					1455:1455	88.2%-92.4%	1445:1455	88.2%-92.4%	1445:1455	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	6	57	theme	detection	1243:1251	arg1	limit					1253:1257	a detection limit	1241:1257	a detection limit of 0.04μgL-1	1241:1270	Under the optimum conditions, the method was exhibited a linear range of 0.1-100μgL-1 (R2=0.9943) for THC with a detection limit of 0.04μgL-1.
27988076	8	58	from	samples	1593:1599	arg1	determination					1547:1559	trace determination	1541:1559	trace determination of THC in the human whole blood samples	1541:1599	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	1	59	from	amount	368:373	arg1	sample					431:436	human whole blood sample	413:436	human whole blood sample	413:436	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	3	60	theme	experimental	836:847	arg1	design					849:854	factorial experimental design	826:854	factorial experimental design	826:854	The volume of eluting solvent, sorbent amount, pH and% NaCl (w/v) influencing on the responses were investigated using factorial experimental design.
27988076	1	61	from	sample	431:436	arg1	THC					403:405	THC	403:405	THC	403:405	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	1	61	from	sample	431:436	arg1	Δ9-tetrahydrocannabinol					378:400	Δ9-tetrahydrocannabinol	378:400	Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection	378:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	1	61	from	sample	431:436	arg1	amount					368:373	trace amount	362:373	trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection	362:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	5	62	theme	eluting	971:977	arg1	solvent					979:985	eluting solvent	971:985	eluting solvent	971:985	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	8	63	theme	satisfactory	1420:1431	arg1	recoveries					1433:1442	The high preconcentration factor (970) and satisfactory recoveries	1377:1442	recoveries	1433:1442	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	2	64	theme	X-ray	647:651	arg1	diffraction					653:663	X-ray diffraction	647:663	X-ray diffraction	647:663	The composite nanofibres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and N2 adsorption-desorption experiments.
27988076	3	65	theme	%	760:760	arg1	NaCl					762:765	% NaCl	760:765	% NaCl	760:765	The volume of eluting solvent, sorbent amount, pH and% NaCl (w/v) influencing on the responses were investigated using factorial experimental design.
27988076	8	66	from	determination	1547:1559	arg1	samples					1593:1599	the human whole blood samples	1571:1599	the human whole blood samples	1571:1599	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	5	67	theme	solvent	979:985	arg1	pH					1007:1008	pH	1007:1008	pH	1007:1008	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	5	67	theme	solvent	979:985	arg1	variables					1027:1035	significant variables	1015:1035	significant variables	1015:1035	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	5	67	theme	solvent	979:985	arg1	volume					961:966	The volume	957:966	The volume of eluting solvent	957:985	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	5	67	theme	solvent	979:985	arg1	solvent					979:985	eluting solvent	971:985	eluting solvent	971:985	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	5	67	theme	solvent	979:985	arg1	amount					996:1001	sorbent amount	988:1001	sorbent amount	988:1001	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	8	68	theme	whole	1461:1465	arg1	samples					1473:1479	whole blood samples	1461:1479	whole blood samples	1461:1479	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	4	69	theme	optimum	861:867	arg1	point					869:873	The optimum point	857:873	The optimum point	857:873	The optimum point was achieved by analysis of the results according to design expert (DX) software.
27988076	5	70	theme	extraction	1109:1118	arg1	response					1120:1127	the best extraction response	1100:1127	the best extraction response	1100:1127	The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response.
27988076	8	71	from	factor	1403:1408	arg1	samples					1473:1479	whole blood samples	1461:1479	whole blood samples	1461:1479	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	0	72	theme	solid-phase	73:83	arg1	extraction					85:94	solid-phase extraction	73:94	solid-phase extraction of Δ9-tetrahydrocannabinol	73:121	Electrospun biocompatible Chitosan/MIL-101 (Fe) composite nanofibers for solid-phase extraction of Δ9-tetrahydrocannabinol in whole blood samples using Box-Behnken experimental design.
27988076	7	73	theme	relative	1333:1340	arg1	deviations					1351:1360	relative standard deviations	1333:1360	relative standard deviations	1333:1360	Acceptable values for intra-day (3.2%) and inter-day (4.8%) relative standard deviations were obtained.
27988076	7	74	theme	Acceptable	1273:1282	arg1	values					1284:1289	Acceptable values	1273:1289	Acceptable values for intra-day (3.2%) and inter-day (4.8%) relative standard deviations	1273:1360	Acceptable values for intra-day (3.2%) and inter-day (4.8%) relative standard deviations were obtained.
27988076	8	75	theme	whole	1581:1585	arg1	samples					1593:1599	the human whole blood samples	1571:1599	the human whole blood samples	1571:1599	The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
27988076	1	76	theme	trace	362:366	arg1	THC					403:405	THC	403:405	THC	403:405	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	1	76	theme	trace	362:366	arg1	Δ9-tetrahydrocannabinol					378:400	Δ9-tetrahydrocannabinol	378:400	Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection	378:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	1	76	theme	trace	362:366	arg1	amount					368:373	trace amount	362:373	trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection	362:526	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
27988076	7	77	theme	standard	1342:1349	arg1	deviations					1351:1360	relative standard deviations	1333:1360	relative standard deviations	1333:1360	Acceptable values for intra-day (3.2%) and inter-day (4.8%) relative standard deviations were obtained.
27988076	6	78	theme	optimum	1140:1146	arg1	conditions					1148:1157	the optimum conditions	1136:1157	the optimum conditions	1136:1157	Under the optimum conditions, the method was exhibited a linear range of 0.1-100μgL-1 (R2=0.9943) for THC with a detection limit of 0.04μgL-1.
27988076	1	79	theme	simple	271:276	arg1	method					315:320	a simple, cheap and accessible electrospining method	269:320	a simple, cheap and accessible electrospining method	269:320	The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection.
29131614	0	0	theme	Biodegradable	79:91	arg1	Poly					93:96	Biodegradable Poly	79:96	Biodegradable Poly(vinyl alcohol)/Starch Film	79:123	Halloysite Tubes as Nanocontainers for Herbicide and Its Controlled Release in Biodegradable Poly(vinyl alcohol)/Starch Film.
29131614	0	0	theme	Biodegradable	79:91	arg1	alcohol					104:110	vinyl alcohol	98:110	vinyl alcohol	98:110	Halloysite Tubes as Nanocontainers for Herbicide and Its Controlled Release in Biodegradable Poly(vinyl alcohol)/Starch Film.
29131614	1	1	theme	AT-loaded	262:270	arg1	HNTs					272:275	the AT-loaded HNTs	258:275	the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST	258:362	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	1	theme	AT-loaded	262:270	arg1	HNTs-AT					278:284	HNTs-AT	278:284	HNTs-AT	278:284	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	3	2	theme	diffusion	840:848	arg1	mechanism					819:827	the mechanism	815:827	the mechanism of Fickian diffusion	815:848	The release behavior of AT from PVA/ST film with HNTs-AT was first dominated by the mechanism of matrix erosion and then by the mechanism of Fickian diffusion.
29131614	1	3	theme	drug	417:420	arg1	system					431:436	a dual drug delivery system	410:436	a dual drug delivery system	410:436	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	4	theme	HNTs	272:275	arg1	lumen					187:191	the lumen	183:191	the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST	183:362	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	3	5	theme	erosion	795:801	arg1	mechanism					775:783	the mechanism	771:783	the mechanism of matrix erosion	771:801	The release behavior of AT from PVA/ST film with HNTs-AT was first dominated by the mechanism of matrix erosion and then by the mechanism of Fickian diffusion.
29131614	3	6	theme	Fickian	832:838	arg1	diffusion					840:848	Fickian diffusion	832:848	Fickian diffusion	832:848	The release behavior of AT from PVA/ST film with HNTs-AT was first dominated by the mechanism of matrix erosion and then by the mechanism of Fickian diffusion.
29131614	2	7	theme	slower	477:482	arg1	release					484:490	much slower release	472:490	much slower release from PVA/ST film in water	472:516	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	8	theme	AT	573:574	arg1	amount					563:568	the total release amount	545:568	the total release amount of AT from PVA/ST film with loaded AT	545:606	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	8	theme	AT	573:574	arg1	%					619:619	only 61%	612:619	only 61%	612:619	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	8	theme	AT	573:574	arg1	AT					573:574	AT	573:574	AT	573:574	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	9	theme	free	682:685	arg1	AT					687:688	free AT	682:688	free AT	682:688	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	0	10	theme	Poly	93:96	arg1	Film					120:123	Biodegradable Poly(vinyl alcohol)/Starch Film	79:123	Biodegradable Poly(vinyl alcohol)/Starch Film	79:123	Halloysite Tubes as Nanocontainers for Herbicide and Its Controlled Release in Biodegradable Poly(vinyl alcohol)/Starch Film.
29131614	1	11	from	lumen	187:191	arg1	amount					231:236	the amount	227:236	the amount of 9 wt %	227:246	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	11	from	lumen	187:191	arg1	%					246:246	9 wt %	241:246	9 wt %	241:246	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	2	12	theme	free	523:526	arg1	AT					528:529	free AT	523:529	free AT	523:529	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	1	13	theme	delivery	422:429	arg1	system					431:436	a dual drug delivery system	410:436	a dual drug delivery system	410:436	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	3	14	theme	matrix	788:793	arg1	erosion					795:801	matrix erosion	788:801	matrix erosion	788:801	The release behavior of AT from PVA/ST film with HNTs-AT was first dominated by the mechanism of matrix erosion and then by the mechanism of Fickian diffusion.
29131614	2	15	with	film	672:675	arg1	AT					687:688	free AT	682:688	free AT	682:688	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	0	16	theme	vinyl	98:102	arg1	Poly					93:96	Biodegradable Poly	79:96	Biodegradable Poly(vinyl alcohol)/Starch Film	79:123	Halloysite Tubes as Nanocontainers for Herbicide and Its Controlled Release in Biodegradable Poly(vinyl alcohol)/Starch Film.
29131614	0	16	theme	vinyl	98:102	arg1	alcohol					104:110	vinyl alcohol	98:110	vinyl alcohol	98:110	Halloysite Tubes as Nanocontainers for Herbicide and Its Controlled Release in Biodegradable Poly(vinyl alcohol)/Starch Film.
29131614	0	17	from	Herbicide	39:47	arg1	Film					120:123	Biodegradable Poly(vinyl alcohol)/Starch Film	79:123	Biodegradable Poly(vinyl alcohol)/Starch Film	79:123	Halloysite Tubes as Nanocontainers for Herbicide and Its Controlled Release in Biodegradable Poly(vinyl alcohol)/Starch Film.
29131614	4	18	theme	soil	983:986	arg1	layer					988:992	the soil layer	979:992	the soil layer	979:992	In addition, combining HNTs and PVA/ST blends together in the controlled release of herbicide also reduced its leaching through the soil layer, which would be useful for diminishing the environmental pollution caused by pesticide.
29131614	4	18	theme	soil	983:986	arg1	useful					1010:1015	useful	1010:1015	useful	1010:1015	In addition, combining HNTs and PVA/ST blends together in the controlled release of herbicide also reduced its leaching through the soil layer, which would be useful for diminishing the environmental pollution caused by pesticide.
29131614	2	19	theme	PVA/ST	665:670	arg1	film					672:675	PVA/ST film	665:675	PVA/ST film with free AT	665:688	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	20	theme	loaded	598:603	arg1	AT					605:606	loaded AT	598:606	loaded AT	598:606	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	21	from	release	484:490	arg1	water					512:516	water	512:516	water	512:516	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	1	22	from	amount	231:236	arg1	lumen					187:191	the lumen	183:191	the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST	183:362	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	3	23	theme	release	695:701	arg1	behavior					703:710	The release behavior	691:710	The release behavior of AT from PVA/ST film with HNTs-AT	691:746	The release behavior of AT from PVA/ST film with HNTs-AT was first dominated by the mechanism of matrix erosion and then by the mechanism of Fickian diffusion.
29131614	1	24	dep	HNTs	272:275	arg1	PVA/ST					357:362	PVA/ST	357:362	PVA/ST	357:362	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	2	25	theme	PVA/ST	497:502	arg1	film					504:507	PVA/ST film	497:507	PVA/ST film	497:507	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	1	26	theme	weight	374:379	arg1	ratio					381:385	the weight ratio	370:385	the weight ratio of 80/20) to construct a dual drug delivery system	370:436	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	3	27	theme	PVA/ST	723:728	arg1	film					730:733	PVA/ST film	723:733	PVA/ST film with HNTs-AT	723:746	The release behavior of AT from PVA/ST film with HNTs-AT was first dominated by the mechanism of matrix erosion and then by the mechanism of Fickian diffusion.
29131614	0	28	theme	/Starch	112:118	arg1	Film					120:123	Biodegradable Poly(vinyl alcohol)/Starch Film	79:123	Biodegradable Poly(vinyl alcohol)/Starch Film	79:123	Halloysite Tubes as Nanocontainers for Herbicide and Its Controlled Release in Biodegradable Poly(vinyl alcohol)/Starch Film.
29131614	4	29	theme	herbicide	935:943	arg1	release					924:930	the controlled release	909:930	the controlled release of herbicide	909:943	In addition, combining HNTs and PVA/ST blends together in the controlled release of herbicide also reduced its leaching through the soil layer, which would be useful for diminishing the environmental pollution caused by pesticide.
29131614	1	30	dep	herbicide	137:145	arg1	AT					157:158	AT	157:158	AT	157:158	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	30	dep	herbicide	137:145	arg1	atrazine					147:154	atrazine	147:154	Commercial herbicide atrazine (AT)	126:159	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	3	31	theme	AT	715:716	arg1	behavior					703:710	The release behavior	691:710	The release behavior of AT from PVA/ST film with HNTs-AT	691:746	The release behavior of AT from PVA/ST film with HNTs-AT was first dominated by the mechanism of matrix erosion and then by the mechanism of Fickian diffusion.
29131614	1	32	theme	wt	243:244	arg1	%					246:246	9 wt %	241:246	9 wt %	241:246	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	4	33	theme	PVA/ST	883:888	arg1	blends					890:895	PVA/ST blends	883:895	PVA/ST blends	883:895	In addition, combining HNTs and PVA/ST blends together in the controlled release of herbicide also reduced its leaching through the soil layer, which would be useful for diminishing the environmental pollution caused by pesticide.
29131614	2	34	from	film	588:591	arg1	amount					563:568	the total release amount	545:568	the total release amount of AT from PVA/ST film with loaded AT	545:606	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	34	from	film	588:591	arg1	%					619:619	only 61%	612:619	only 61%	612:619	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	34	from	film	588:591	arg1	AT					573:574	AT	573:574	AT	573:574	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	1	35	theme	%	246:246	arg1	amount					231:236	the amount	227:236	the amount of 9 wt %	227:246	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	35	theme	%	246:246	arg1	%					246:246	9 wt %	241:246	9 wt %	241:246	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	36	theme	poly	318:321	arg1	composites					345:354	poly(vinyl alcohol)/starch composites	318:354	poly(vinyl alcohol)/starch composites	318:354	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	2	37	with	film	588:591	arg1	AT					605:606	loaded AT	598:606	loaded AT	598:606	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	38	from	film	504:507	arg1	release					484:490	much slower release	472:490	much slower release from PVA/ST film in water	472:516	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	1	39	from	nanotubes	207:215	arg1	amount					231:236	the amount	227:236	the amount of 9 wt %	227:246	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	39	from	nanotubes	207:215	arg1	%					246:246	9 wt %	241:246	9 wt %	241:246	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	3	40	with	film	730:733	arg1	HNTs-AT					740:746	HNTs-AT	740:746	HNTs-AT	740:746	The release behavior of AT from PVA/ST film with HNTs-AT was first dominated by the mechanism of matrix erosion and then by the mechanism of Fickian diffusion.
29131614	1	41	theme	80/20	390:394	arg1	ratio					381:385	the weight ratio	370:385	the weight ratio of 80/20) to construct a dual drug delivery system	370:436	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	42	theme	vinyl	323:327	arg1	poly					318:321	poly	318:321	poly(vinyl alcohol)/starch composites	318:354	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	42	theme	vinyl	323:327	arg1	alcohol					329:335	vinyl alcohol	323:335	vinyl alcohol	323:335	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	3	43	from	film	730:733	arg1	behavior					703:710	The release behavior	691:710	The release behavior of AT from PVA/ST film with HNTs-AT	691:746	The release behavior of AT from PVA/ST film with HNTs-AT was first dominated by the mechanism of matrix erosion and then by the mechanism of Fickian diffusion.
29131614	2	44	theme	release	555:561	arg1	amount					563:568	the total release amount	545:568	the total release amount of AT from PVA/ST film with loaded AT	545:606	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	44	theme	release	555:561	arg1	%					619:619	only 61%	612:619	only 61%	612:619	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	44	theme	release	555:561	arg1	AT					573:574	AT	573:574	AT	573:574	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	0	45	from	Release	68:74	arg1	Film					120:123	Biodegradable Poly(vinyl alcohol)/Starch Film	79:123	Biodegradable Poly(vinyl alcohol)/Starch Film	79:123	Halloysite Tubes as Nanocontainers for Herbicide and Its Controlled Release in Biodegradable Poly(vinyl alcohol)/Starch Film.
29131614	2	46	theme	total	549:553	arg1	amount					563:568	the total release amount	545:568	the total release amount of AT from PVA/ST film with loaded AT	545:606	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	46	theme	total	549:553	arg1	%					619:619	only 61%	612:619	only 61%	612:619	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	2	46	theme	total	549:553	arg1	AT					573:574	AT	573:574	AT	573:574	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	4	47	theme	environmental	1037:1049	arg1	pollution					1051:1059	the environmental pollution	1033:1059	the environmental pollution caused by pesticide	1033:1079	In addition, combining HNTs and PVA/ST blends together in the controlled release of herbicide also reduced its leaching through the soil layer, which would be useful for diminishing the environmental pollution caused by pesticide.
29131614	4	48	theme	controlled	913:922	arg1	release					924:930	the controlled release	909:930	the controlled release of herbicide	909:943	In addition, combining HNTs and PVA/ST blends together in the controlled release of herbicide also reduced its leaching through the soil layer, which would be useful for diminishing the environmental pollution caused by pesticide.
29131614	1	49	theme	halloysite	196:205	arg1	nanotubes					207:215	halloysite nanotubes	196:215	halloysite nanotubes (HNTs) in the amount of 9 wt %	196:246	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	49	theme	halloysite	196:205	arg1	HNTs					218:221	HNTs	218:221	HNTs	218:221	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	50	theme	/starch	337:343	arg1	composites					345:354	poly(vinyl alcohol)/starch composites	318:354	poly(vinyl alcohol)/starch composites	318:354	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	2	51	theme	PVA/ST	581:586	arg1	film					588:591	PVA/ST film	581:591	PVA/ST film with loaded AT	581:606	AT loaded in nanotubes displayed much slower release from PVA/ST film in water than free AT; for example, the total release amount of AT from PVA/ST film with loaded AT was only 61% after 96 h, while this value reached 97% in PVA/ST film with free AT.
29131614	1	52	theme	Commercial	126:135	arg1	herbicide					137:145	Commercial herbicide	126:145	Commercial herbicide atrazine (AT)	126:159	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	1	53	theme	nanotubes	207:215	arg1	lumen					187:191	the lumen	183:191	the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST	183:362	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
29131614	0	54	theme	Controlled	57:66	arg1	Release					68:74	Its Controlled Release	53:74	Its Controlled Release in Biodegradable Poly(vinyl alcohol)/Starch Film	53:123	Halloysite Tubes as Nanocontainers for Herbicide and Its Controlled Release in Biodegradable Poly(vinyl alcohol)/Starch Film.
29131614	1	55	theme	dual	412:415	arg1	system					431:436	a dual drug delivery system	410:436	a dual drug delivery system	410:436	Commercial herbicide atrazine (AT) was first loaded into the lumen of halloysite nanotubes (HNTs) in the amount of 9 wt %, and then the AT-loaded HNTs (HNTs-AT) were further incorporated into poly(vinyl alcohol)/starch composites (PVA/ST, with the weight ratio of 80/20) to construct a dual drug delivery system.
28205316	0	0	theme	selective	91:99	arg1	extraction					101:110	the highly selective extraction	80:110	the highly selective extraction of methyl jasmonate	80:130	Novel polymeric monolith materials with a β-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate.
28205316	4	1	theme	methyl	714:719	arg1	jasmonate					721:729	methyl jasmonate	714:729	methyl jasmonate	714:729	In addition, the method validation showed a wide linear range of 5-2000 ng/mL, with a good linearity and low limits of detection for methyl jasmonate.
28205316	3	2	theme	sample	534:539	arg1	value					544:548	sample pH value	534:548	sample pH value	534:548	To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume, and sample pH value were systematically optimized.
28205316	3	3	theme	pH	541:542	arg1	value					544:548	sample pH value	534:548	sample pH value	534:548	To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume, and sample pH value were systematically optimized.
28205316	5	4	from	jasmonate	809:817	arg1	flowers					834:840	wintersweet flowers	822:840	wintersweet flowers	822:840	The proposed method was successfully applied for the determination of methyl jasmonate in wintersweet flowers with recoveries of 90.67%.
28205316	4	5	theme	wide	625:628	arg1	range					637:641	a wide linear range	623:641	a wide linear range of 5-2000 ng/mL	623:657	In addition, the method validation showed a wide linear range of 5-2000 ng/mL, with a good linearity and low limits of detection for methyl jasmonate.
28205316	1	6	with	column	160:165	arg1	composite					198:206	a β-cyclodextrin-graphene composite	172:206	a β-cyclodextrin-graphene composite	172:206	A novel polymeric monolith column with a β-cyclodextrin-graphene composite was prepared for extraction of methyl jasmonate.
28205316	2	7	theme	monolith	302:309	arg1	microextraction					311:325	A simple, sensitive, and effective polymeric monolith microextraction	257:325	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method	257:376	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method has been presented for the determination.
28205316	4	8	theme	good	667:670	arg1	linearity					672:680	a good linearity	665:680	a good linearity	665:680	In addition, the method validation showed a wide linear range of 5-2000 ng/mL, with a good linearity and low limits of detection for methyl jasmonate.
28205316	0	9	theme	jasmonate	122:130	arg1	extraction					101:110	the highly selective extraction	80:110	the highly selective extraction of methyl jasmonate	80:130	Novel polymeric monolith materials with a β-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate.
28205316	5	10	theme	%	866:866	arg1	recoveries					847:856	recoveries	847:856	recoveries of 90.67%	847:866	The proposed method was successfully applied for the determination of methyl jasmonate in wintersweet flowers with recoveries of 90.67%.
28205316	1	11	theme	β-cyclodextrin-graphene	174:196	arg1	composite					198:206	a β-cyclodextrin-graphene composite	172:206	a β-cyclodextrin-graphene composite	172:206	A novel polymeric monolith column with a β-cyclodextrin-graphene composite was prepared for extraction of methyl jasmonate.
28205316	0	12	theme	methyl	115:120	arg1	jasmonate					122:130	methyl jasmonate	115:130	methyl jasmonate	115:130	Novel polymeric monolith materials with a β-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate.
28205316	5	13	from	flowers	834:840	arg1	determination					785:797	the determination	781:797	the determination of methyl jasmonate in wintersweet flowers with recoveries of 90.67%	781:866	The proposed method was successfully applied for the determination of methyl jasmonate in wintersweet flowers with recoveries of 90.67%.
28205316	2	14	theme	effective	282:290	arg1	microextraction					311:325	A simple, sensitive, and effective polymeric monolith microextraction	257:325	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method	257:376	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method has been presented for the determination.
28205316	0	15	theme	polymeric	6:14	arg1	materials					25:33	Novel polymeric monolith materials	0:33	Novel polymeric monolith materials with a β-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate.	0:131	Novel polymeric monolith materials with a β-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate.
28205316	6	16	theme	high-performance	897:912	arg1	chromatography					921:934	high-performance liquid chromatography	897:934	high-performance liquid chromatography with mass spectrometry	897:957	The result was confirmed by high-performance liquid chromatography with mass spectrometry.
28205316	2	17	theme	chromatography	356:369	arg1	method					371:376	high-performance liquid chromatography method	332:376	high-performance liquid chromatography method	332:376	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method has been presented for the determination.
28205316	3	18	theme	several	470:476	arg1	rate					509:512	sample flow rate	497:512	sample flow rate	497:512	To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume, and sample pH value were systematically optimized.
28205316	3	18	theme	several	470:476	arg1	value					544:548	sample pH value	534:548	sample pH value	534:548	To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume, and sample pH value were systematically optimized.
28205316	3	18	theme	several	470:476	arg1	parameters					478:487	several parameters	470:487	several parameters such as sample flow rate, sample volume, and sample pH value	470:548	To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume, and sample pH value were systematically optimized.
28205316	3	18	theme	several	470:476	arg1	volume					522:527	sample volume	515:527	sample volume	515:527	To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume, and sample pH value were systematically optimized.
28205316	0	19	theme	Novel	0:4	arg1	materials					25:33	Novel polymeric monolith materials	0:33	Novel polymeric monolith materials with a β-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate.	0:131	Novel polymeric monolith materials with a β-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate.
28205316	2	20	theme	liquid	349:354	arg1	chromatography					356:369	high-performance liquid chromatography	332:369	high-performance liquid chromatography method	332:376	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method has been presented for the determination.
28205316	4	21	theme	low	686:688	arg1	limits					690:695	low limits	686:695	low limits	686:695	In addition, the method validation showed a wide linear range of 5-2000 ng/mL, with a good linearity and low limits of detection for methyl jasmonate.
28205316	4	22	theme	linear	630:635	arg1	range					637:641	a wide linear range	623:641	a wide linear range of 5-2000 ng/mL	623:657	In addition, the method validation showed a wide linear range of 5-2000 ng/mL, with a good linearity and low limits of detection for methyl jasmonate.
28205316	2	23	theme	high-performance	332:347	arg1	chromatography					356:369	high-performance liquid chromatography	332:369	high-performance liquid chromatography method	332:376	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method has been presented for the determination.
28205316	6	24	theme	mass	941:944	arg1	spectrometry					946:957	mass spectrometry	941:957	mass spectrometry	941:957	The result was confirmed by high-performance liquid chromatography with mass spectrometry.
28205316	0	25	theme	monolith	16:23	arg1	materials					25:33	Novel polymeric monolith materials	0:33	Novel polymeric monolith materials with a β-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate.	0:131	Novel polymeric monolith materials with a β-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate.
28205316	4	26	theme	method	598:603	arg1	validation					605:614	the method validation	594:614	the method validation	594:614	In addition, the method validation showed a wide linear range of 5-2000 ng/mL, with a good linearity and low limits of detection for methyl jasmonate.
28205316	6	27	with	chromatography	921:934	arg1	spectrometry					946:957	mass spectrometry	941:957	mass spectrometry	941:957	The result was confirmed by high-performance liquid chromatography with mass spectrometry.
28205316	4	28	theme	detection	700:708	arg1	linearity					672:680	a good linearity	665:680	a good linearity	665:680	In addition, the method validation showed a wide linear range of 5-2000 ng/mL, with a good linearity and low limits of detection for methyl jasmonate.
28205316	4	28	theme	detection	700:708	arg1	limits					690:695	low limits	686:695	low limits	686:695	In addition, the method validation showed a wide linear range of 5-2000 ng/mL, with a good linearity and low limits of detection for methyl jasmonate.
28205316	5	29	theme	methyl	802:807	arg1	jasmonate					809:817	methyl jasmonate	802:817	methyl jasmonate in wintersweet flowers with recoveries of 90.67%	802:866	The proposed method was successfully applied for the determination of methyl jasmonate in wintersweet flowers with recoveries of 90.67%.
28205316	3	30	theme	sample	497:502	arg1	rate					509:512	sample flow rate	497:512	sample flow rate	497:512	To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume, and sample pH value were systematically optimized.
28205316	2	31	theme	simple	259:264	arg1	microextraction					311:325	A simple, sensitive, and effective polymeric monolith microextraction	257:325	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method	257:376	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method has been presented for the determination.
28205316	2	32	dep	simple	259:264	arg1	polymeric					292:300	polymeric	292:300	polymeric	292:300	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method has been presented for the determination.
28205316	5	33	theme	jasmonate	809:817	arg1	determination					785:797	the determination	781:797	the determination of methyl jasmonate in wintersweet flowers with recoveries of 90.67%	781:866	The proposed method was successfully applied for the determination of methyl jasmonate in wintersweet flowers with recoveries of 90.67%.
28205316	5	34	theme	proposed	736:743	arg1	method					745:750	The proposed method	732:750	The proposed method	732:750	The proposed method was successfully applied for the determination of methyl jasmonate in wintersweet flowers with recoveries of 90.67%.
28205316	4	35	theme	5-2000 ng/mL	646:657	arg1	range					637:641	a wide linear range	623:641	a wide linear range of 5-2000 ng/mL	623:657	In addition, the method validation showed a wide linear range of 5-2000 ng/mL, with a good linearity and low limits of detection for methyl jasmonate.
28205316	0	36	with	materials	25:33	arg1	composite					66:74	a β-cyclodextrin-graphene composite	40:74	a β-cyclodextrin-graphene composite	40:74	Novel polymeric monolith materials with a β-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate.
28205316	1	37	theme	methyl	239:244	arg1	jasmonate					246:254	methyl jasmonate	239:254	methyl jasmonate	239:254	A novel polymeric monolith column with a β-cyclodextrin-graphene composite was prepared for extraction of methyl jasmonate.
28205316	3	38	theme	best	437:440	arg1	efficiency					458:467	the best microextraction efficiency	433:467	the best microextraction efficiency	433:467	To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume, and sample pH value were systematically optimized.
28205316	5	39	theme	wintersweet	822:832	arg1	flowers					834:840	wintersweet flowers	822:840	wintersweet flowers	822:840	The proposed method was successfully applied for the determination of methyl jasmonate in wintersweet flowers with recoveries of 90.67%.
28205316	6	40	theme	liquid	914:919	arg1	chromatography					921:934	high-performance liquid chromatography	897:934	high-performance liquid chromatography with mass spectrometry	897:957	The result was confirmed by high-performance liquid chromatography with mass spectrometry.
28205316	0	41	theme	β-cyclodextrin-graphene	42:64	arg1	composite					66:74	a β-cyclodextrin-graphene composite	40:74	a β-cyclodextrin-graphene composite	40:74	Novel polymeric monolith materials with a β-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate.
28205316	5	42	from	determination	785:797	arg1	flowers					834:840	wintersweet flowers	822:840	wintersweet flowers	822:840	The proposed method was successfully applied for the determination of methyl jasmonate in wintersweet flowers with recoveries of 90.67%.
28205316	3	43	theme	microextraction	442:456	arg1	efficiency					458:467	the best microextraction efficiency	433:467	the best microextraction efficiency	433:467	To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume, and sample pH value were systematically optimized.
28205316	1	44	theme	novel	135:139	arg1	column					160:165	A novel polymeric monolith column	133:165	A novel polymeric monolith column with a β-cyclodextrin-graphene composite	133:206	A novel polymeric monolith column with a β-cyclodextrin-graphene composite was prepared for extraction of methyl jasmonate.
28205316	1	45	theme	jasmonate	246:254	arg1	extraction					225:234	extraction	225:234	extraction of methyl jasmonate	225:254	A novel polymeric monolith column with a β-cyclodextrin-graphene composite was prepared for extraction of methyl jasmonate.
28205316	5	46	with	jasmonate	809:817	arg1	recoveries					847:856	recoveries	847:856	recoveries of 90.67%	847:866	The proposed method was successfully applied for the determination of methyl jasmonate in wintersweet flowers with recoveries of 90.67%.
28205316	1	47	theme	polymeric	141:149	arg1	column					160:165	A novel polymeric monolith column	133:165	A novel polymeric monolith column with a β-cyclodextrin-graphene composite	133:206	A novel polymeric monolith column with a β-cyclodextrin-graphene composite was prepared for extraction of methyl jasmonate.
28205316	3	48	theme	sample	515:520	arg1	volume					522:527	sample volume	515:527	sample volume	515:527	To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume, and sample pH value were systematically optimized.
28205316	3	49	theme	flow	504:507	arg1	rate					509:512	sample flow rate	497:512	sample flow rate	497:512	To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume, and sample pH value were systematically optimized.
28205316	2	50	theme	sensitive	267:275	arg1	microextraction					311:325	A simple, sensitive, and effective polymeric monolith microextraction	257:325	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method	257:376	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method has been presented for the determination.
28205316	1	51	theme	monolith	151:158	arg1	column					160:165	A novel polymeric monolith column	133:165	A novel polymeric monolith column with a β-cyclodextrin-graphene composite	133:206	A novel polymeric monolith column with a β-cyclodextrin-graphene composite was prepared for extraction of methyl jasmonate.
28205316	2	52	with	microextraction	311:325	arg1	method					371:376	high-performance liquid chromatography method	332:376	high-performance liquid chromatography method	332:376	A simple, sensitive, and effective polymeric monolith microextraction with high-performance liquid chromatography method has been presented for the determination.
25940526	4	0	theme	0.89	806:809	arg1	range					789:793	the range	785:793	the range of 0.45 to 0.89	785:809	The release exponent 'n' for films with different compositions was found in the range of 0.45 to 0.89, thus indicating non-Fickian release mechanism.
25940526	1	1	theme	buffer	314:319	arg1	solution					321:328	physiological buffer solution	300:328	physiological buffer solution (PBS)	300:334	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	1	1	theme	buffer	314:319	arg1	PBS					331:333	PBS	331:333	PBS	331:333	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	6	2	theme	g	1076:1076	arg1	formation					1033:1041	thrombus formation	1024:1041	thrombus formation of 0.010±0.001 g and 0.007±0.001 g	1024:1076	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	9	3	theme	antibacterial	1500:1512	arg1	properties					1514:1523	fair anti-fungal and antibacterial properties	1479:1523	fair anti-fungal and antibacterial properties	1479:1523	Finally, the Mic-loaded films showed fair anti-fungal and antibacterial properties.
25940526	4	4	dep	0.89	806:809	arg1	to					803:804	to	803:804	to	803:804	The release exponent 'n' for films with different compositions was found in the range of 0.45 to 0.89, thus indicating non-Fickian release mechanism.
25940526	1	5	theme	hydrogel	217:224	arg1	films					226:230	antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films	123:230	antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films	123:230	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	6	6	theme	non-thrombogenic	1110:1125	arg1	behavior					1127:1134	the non-thrombogenic behavior	1106:1134	the non-thrombogenic behavior	1106:1134	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	2	7	theme	Mic	429:431	arg1	release					433:439	Mic release	429:439	Mic release of 2500 μg/g	429:452	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	4	8	theme	release	713:719	arg1	exponent					721:728	The release exponent	709:728	The release exponent 'n' for films with different compositions	709:770	The release exponent 'n' for films with different compositions was found in the range of 0.45 to 0.89, thus indicating non-Fickian release mechanism.
25940526	0	9	theme	wound	93:97	arg1	dressing					99:106	wound dressing	93:106	wound dressing	93:106	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel films as wound dressing.
25940526	3	10	theme	N	592:592	arg1	concentration					562:574	the concentration	558:574	the concentration of cross-linker N	558:592	In addition, with the increase in the concentration of cross-linker N,N'-methylene bisacrylamide (MB) from to, the drug release from the resulting films decreased from 507 to 191 μg/g.
25940526	6	11	theme	g	1058:1058	arg1	formation					1033:1041	thrombus formation	1024:1041	thrombus formation of 0.010±0.001 g and 0.007±0.001 g	1024:1076	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	2	12	theme	release	515:521	arg1	μg/g					502:505	3100 μg/g	497:505	3100 μg/g of drug release	497:521	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	8	13	theme	adsorption	1430:1439	arg1	degree					1412:1417	a fair degree	1405:1417	a fair degree of protein adsorption	1405:1439	In addition, both of the film samples demonstrated protein adsorption of 49.02±0.59μ g/μL and 51.20±0.51 μg/μL per cm(2), thus revealing a fair degree of protein adsorption.
25940526	3	14	theme	resulting	661:669	arg1	films					671:675	the resulting films	657:675	the resulting films	657:675	In addition, with the increase in the concentration of cross-linker N,N'-methylene bisacrylamide (MB) from to, the drug release from the resulting films decreased from 507 to 191 μg/g.
25940526	5	15	theme	release	919:925	arg1	data					927:930	the kinetic drug release data	902:930	the kinetic drug release data	902:930	The Schott model was employed to interpret the kinetic drug release data successfully.
25940526	2	16	theme	drug	510:513	arg1	release					515:521	drug release	510:521	drug release	510:521	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	7	17	dep	Hemolysis	1148:1156	arg1	both					1159:1162	both	1159:1162	both	1159:1162	In percent Hemolysis, both of the film samples of 1.136±0.012 and 0.5±0.020, respectively, thus indicating non-hemolytic behavior.
25940526	2	18	theme	CNWs	410:413	arg1	w/w					402:404	w/w	402:404	w/w	402:404	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	2	18	theme	CNWs	410:413	arg1	CNWs					410:413	CNWs	410:413	CNWs	410:413	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	2	18	theme	CNWs	410:413	arg1	%					399:399	9.7%	396:399	9.7% (w/w) of CNWs	396:413	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	8	19	theme	protein	1319:1325	arg1	adsorption					1327:1336	protein adsorption	1319:1336	protein adsorption of 49.02±0.59μ g/μL	1319:1356	In addition, both of the film samples demonstrated protein adsorption of 49.02±0.59μ g/μL and 51.20±0.51 μg/μL per cm(2), thus revealing a fair degree of protein adsorption.
25940526	3	20	from	increase	546:553	arg1	concentration					562:574	the concentration	558:574	the concentration of cross-linker N	558:592	In addition, with the increase in the concentration of cross-linker N,N'-methylene bisacrylamide (MB) from to, the drug release from the resulting films decreased from 507 to 191 μg/g.
25940526	4	21	theme	release	840:846	arg1	mechanism					848:856	non-Fickian release mechanism	828:856	non-Fickian release mechanism	828:856	The release exponent 'n' for films with different compositions was found in the range of 0.45 to 0.89, thus indicating non-Fickian release mechanism.
25940526	1	22	theme	loaded	157:162	arg1	CNWs					189:192	CNWs	189:192	CNWs	189:192	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	1	22	theme	loaded	157:162	arg1	nano-whiskers					174:186	loaded cellulose nano-whiskers	157:186	antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films	123:230	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	0	23	theme	cellulose	19:27	arg1	hydrogel					75:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel films as wound dressing.
25940526	3	24	theme	cross-linker	579:590	arg1	N					592:592	cross-linker N	579:592	cross-linker N	579:592	In addition, with the increase in the concentration of cross-linker N,N'-methylene bisacrylamide (MB) from to, the drug release from the resulting films decreased from 507 to 191 μg/g.
25940526	2	25	contain	containing	385:394	arg2	%					399:399	9.7%	396:399	9.7% (w/w) of CNWs	396:413	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	2	25	contain	containing	385:394	arg1	film					379:382	The (CNWs)/poly(sodium acrylate) film	346:382	The (CNWs)/poly(sodium acrylate) film	346:382	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	2	25	contain	containing	385:394	arg2	CNWs					410:413	CNWs	410:413	CNWs	410:413	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	2	25	contain	containing	385:394	arg2	w/w					402:404	w/w	402:404	w/w	402:404	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	9	26	theme	anti-fungal	1484:1494	arg1	properties					1514:1523	fair anti-fungal and antibacterial properties	1479:1523	fair anti-fungal and antibacterial properties	1479:1523	Finally, the Mic-loaded films showed fair anti-fungal and antibacterial properties.
25940526	7	27	theme	non-hemolytic	1244:1256	arg1	behavior					1258:1265	non-hemolytic behavior	1244:1265	non-hemolytic behavior	1244:1265	In percent Hemolysis, both of the film samples of 1.136±0.012 and 0.5±0.020, respectively, thus indicating non-hemolytic behavior.
25940526	1	28	theme	cellulose	164:172	arg1	CNWs					189:192	CNWs	189:192	CNWs	189:192	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	1	28	theme	cellulose	164:172	arg1	nano-whiskers					174:186	loaded cellulose nano-whiskers	157:186	antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films	123:230	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	0	29	theme	Minocycline-loaded	0:17	arg1	hydrogel					75:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel films as wound dressing.
25940526	6	30	theme	thrombus	1024:1031	arg1	formation					1033:1041	thrombus formation	1024:1041	thrombus formation of 0.010±0.001 g and 0.007±0.001 g	1024:1076	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	7	31	theme	1.136±0.012	1187:1197	arg1	samples					1176:1182	the film samples	1167:1182	the film samples of 1.136±0.012 and 0.5±0.020, respectively	1167:1225	In percent Hemolysis, both of the film samples of 1.136±0.012 and 0.5±0.020, respectively, thus indicating non-hemolytic behavior.
25940526	4	32	with	films	738:742	arg1	compositions					759:770	different compositions	749:770	different compositions	749:770	The release exponent 'n' for films with different compositions was found in the range of 0.45 to 0.89, thus indicating non-Fickian release mechanism.
25940526	8	33	theme	film	1293:1296	arg1	samples					1298:1304	the film samples	1289:1304	the film samples	1289:1304	In addition, both of the film samples demonstrated protein adsorption of 49.02±0.59μ g/μL and 51.20±0.51 μg/μL per cm(2), thus revealing a fair degree of protein adsorption.
25940526	0	34	theme	whiskers/poly	34:46	arg1	hydrogel					75:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel films as wound dressing.
25940526	6	35	dep	containing	1000:1009	arg1	both					991:994	both	991:994	both	991:994	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	6	35	dep	containing	1000:1009	arg1	not					996:998	not	996:998	not	996:998	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	2	36	theme	μg/g	449:452	arg1	release					433:439	Mic release	429:439	Mic release of 2500 μg/g	429:452	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	3	37	dep	191	699:701	arg1	to					696:697	to	696:697	to	696:697	In addition, with the increase in the concentration of cross-linker N,N'-methylene bisacrylamide (MB) from to, the drug release from the resulting films decreased from 507 to 191 μg/g.
25940526	7	38	theme	film	1171:1174	arg1	samples					1176:1182	the film samples	1167:1182	the film samples of 1.136±0.012 and 0.5±0.020, respectively	1167:1225	In percent Hemolysis, both of the film samples of 1.136±0.012 and 0.5±0.020, respectively, thus indicating non-hemolytic behavior.
25940526	6	39	dep	CNWs/poly	976:984	arg1	containing					1000:1009	containing	1000:1009	containing drug	1000:1014	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	0	40	theme	nano	29:32	arg1	hydrogel					75:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel films as wound dressing.
25940526	3	41	theme	N'-methylene	594:605	arg1	increase					546:553	the increase	542:553	the increase in the concentration of cross-linker N	542:592	In addition, with the increase in the concentration of cross-linker N,N'-methylene bisacrylamide (MB) from to, the drug release from the resulting films decreased from 507 to 191 μg/g.
25940526	3	41	theme	N'-methylene	594:605	arg1	MB					622:623	MB	622:623	MB	622:623	In addition, with the increase in the concentration of cross-linker N,N'-methylene bisacrylamide (MB) from to, the drug release from the resulting films decreased from 507 to 191 μg/g.
25940526	3	41	theme	N'-methylene	594:605	arg1	bisacrylamide					607:619	N'-methylene bisacrylamide	594:619	N'-methylene bisacrylamide (MB) from to	594:632	In addition, with the increase in the concentration of cross-linker N,N'-methylene bisacrylamide (MB) from to, the drug release from the resulting films decreased from 507 to 191 μg/g.
25940526	2	42	theme	2500	444:447	arg1	μg/g					449:452	2500 μg/g	444:452	2500 μg/g	444:452	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	1	43	dep	Minocycline	139:149	arg1	CNWs					189:192	CNWs	189:192	CNWs	189:192	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	1	43	dep	Minocycline	139:149	arg1	nano-whiskers					174:186	loaded cellulose nano-whiskers	157:186	antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films	123:230	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	8	44	theme	g/μL	1353:1356	arg1	adsorption					1327:1336	protein adsorption	1319:1336	protein adsorption of 49.02±0.59μ g/μL	1319:1356	In addition, both of the film samples demonstrated protein adsorption of 49.02±0.59μ g/μL and 51.20±0.51 μg/μL per cm(2), thus revealing a fair degree of protein adsorption.
25940526	8	44	theme	g/μL	1353:1356	arg1	μg/μL					1373:1377	51.20±0.51 μg/μL	1362:1377	51.20±0.51 μg/μL per cm(2)	1362:1387	In addition, both of the film samples demonstrated protein adsorption of 49.02±0.59μ g/μL and 51.20±0.51 μg/μL per cm(2), thus revealing a fair degree of protein adsorption.
25940526	0	45	theme	sodium	48:53	arg1	hydrogel					75:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel films as wound dressing.
25940526	8	46	theme	49.02±0.59μ	1341:1351	arg1	g/μL					1353:1356	49.02±0.59μ g/μL	1341:1356	49.02±0.59μ g/μL	1341:1356	In addition, both of the film samples demonstrated protein adsorption of 49.02±0.59μ g/μL and 51.20±0.51 μg/μL per cm(2), thus revealing a fair degree of protein adsorption.
25940526	3	47	from	films	671:675	arg1	release					644:650	the drug release	635:650	the drug release from the resulting films	635:675	In addition, with the increase in the concentration of cross-linker N,N'-methylene bisacrylamide (MB) from to, the drug release from the resulting films decreased from 507 to 191 μg/g.
25940526	9	48	theme	fair	1479:1482	arg1	properties					1514:1523	fair anti-fungal and antibacterial properties	1479:1523	fair anti-fungal and antibacterial properties	1479:1523	Finally, the Mic-loaded films showed fair anti-fungal and antibacterial properties.
25940526	2	49	theme	CNWs	351:354	arg1	film					379:382	The (CNWs)/poly(sodium acrylate) film	346:382	The (CNWs)/poly(sodium acrylate) film	346:382	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	4	50	theme	non-Fickian	828:838	arg1	mechanism					848:856	non-Fickian release mechanism	828:856	non-Fickian release mechanism	828:856	The release exponent 'n' for films with different compositions was found in the range of 0.45 to 0.89, thus indicating non-Fickian release mechanism.
25940526	2	51	theme	poly	470:473	arg1	film					485:488	the plain poly(acrylate) film	460:488	the plain poly(acrylate) film	460:488	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	5	52	theme	kinetic	906:912	arg1	data					927:930	the kinetic drug release data	902:930	the kinetic drug release data	902:930	The Schott model was employed to interpret the kinetic drug release data successfully.
25940526	0	53	theme	acrylate	55:62	arg1	hydrogel					75:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel films as wound dressing.
25940526	2	54	theme	plain	464:468	arg1	acrylate					475:482	acrylate	475:482	acrylate	475:482	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	2	54	theme	plain	464:468	arg1	poly					470:473	plain poly	464:473	the plain poly(acrylate) film	460:488	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	5	55	theme	Schott	863:868	arg1	model					870:874	The Schott model	859:874	The Schott model	859:874	The Schott model was employed to interpret the kinetic drug release data successfully.
25940526	5	56	theme	drug	914:917	arg1	data					927:930	the kinetic drug release data	902:930	the kinetic drug release data	902:930	The Schott model was employed to interpret the kinetic drug release data successfully.
25940526	6	57	theme	film	950:953	arg1	CNWs/poly					976:984	CNWs/poly	976:984	CNWs/poly(SA) (both not containing drug)	976:1015	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	6	57	theme	film	950:953	arg1	poly					963:966	poly	963:966	poly(SA)	963:970	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	6	57	theme	film	950:953	arg1	samples					955:961	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug)	946:1015	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug)	946:1015	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	7	58	theme	percent	1140:1146	arg1	Hemolysis					1148:1156	percent Hemolysis	1140:1156	percent Hemolysis	1140:1156	In percent Hemolysis, both of the film samples of 1.136±0.012 and 0.5±0.020, respectively, thus indicating non-hemolytic behavior.
25940526	8	59	theme	fair	1407:1410	arg1	degree					1412:1417	a fair degree	1405:1417	a fair degree of protein adsorption	1405:1439	In addition, both of the film samples demonstrated protein adsorption of 49.02±0.59μ g/μL and 51.20±0.51 μg/μL per cm(2), thus revealing a fair degree of protein adsorption.
25940526	7	60	theme	0.5±0.020	1203:1211	arg1	samples					1176:1182	the film samples	1167:1182	the film samples of 1.136±0.012 and 0.5±0.020, respectively	1167:1225	In percent Hemolysis, both of the film samples of 1.136±0.012 and 0.5±0.020, respectively, thus indicating non-hemolytic behavior.
25940526	4	61	located	found	776:780	arg2	exponent					721:728	The release exponent	709:728	The release exponent 'n' for films with different compositions	709:770	The release exponent 'n' for films with different compositions was found in the range of 0.45 to 0.89, thus indicating non-Fickian release mechanism.
25940526	4	61	located	found	776:780	arg1	range					789:793	the range	785:793	the range of 0.45 to 0.89	785:809	The release exponent 'n' for films with different compositions was found in the range of 0.45 to 0.89, thus indicating non-Fickian release mechanism.
25940526	1	62	theme	antibiotic	123:132	arg1	acrylate					207:214	sodium acrylate	200:214	sodium acrylate	200:214	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	1	62	theme	antibiotic	123:132	arg1	Minocycline					139:149	antibiotic drug Minocycline	123:149	antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films	123:230	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	1	62	theme	antibiotic	123:132	arg1	Mic					152:154	Mic	152:154	Mic	152:154	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	8	63	theme	protein	1422:1428	arg1	adsorption					1430:1439	protein adsorption	1422:1439	protein adsorption	1422:1439	In addition, both of the film samples demonstrated protein adsorption of 49.02±0.59μ g/μL and 51.20±0.51 μg/μL per cm(2), thus revealing a fair degree of protein adsorption.
25940526	1	64	theme	sodium	200:205	arg1	acrylate					207:214	sodium acrylate	200:214	sodium acrylate	200:214	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	1	64	theme	sodium	200:205	arg1	Minocycline					139:149	antibiotic drug Minocycline	123:149	antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films	123:230	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	4	65	theme	different	749:757	arg1	compositions					759:770	different compositions	749:770	different compositions	749:770	The release exponent 'n' for films with different compositions was found in the range of 0.45 to 0.89, thus indicating non-Fickian release mechanism.
25940526	6	66	dep	samples	955:961	arg1	SA					968:969	SA	968:969	SA	968:969	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	6	66	dep	samples	955:961	arg1	CNWs/poly					976:984	CNWs/poly	976:984	CNWs/poly(SA) (both not containing drug)	976:1015	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	6	66	dep	samples	955:961	arg1	poly					963:966	poly	963:966	poly(SA)	963:970	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	6	66	dep	samples	955:961	arg1	samples					955:961	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug)	946:1015	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug)	946:1015	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	6	66	dep	samples	955:961	arg1	SA					986:987	SA	986:987	SA	986:987	The film samples poly(SA) and CNWs/poly(SA) (both not containing drug) showed thrombus formation of 0.010±0.001 g and 0.007±0.001 g, respectively, thus showing the non-thrombogenic behavior.
25940526	1	67	theme	drug	134:137	arg1	acrylate					207:214	sodium acrylate	200:214	sodium acrylate	200:214	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	1	67	theme	drug	134:137	arg1	Minocycline					139:149	antibiotic drug Minocycline	123:149	antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films	123:230	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	1	67	theme	drug	134:137	arg1	Mic					152:154	Mic	152:154	Mic	152:154	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	0	68	theme	composite	65:73	arg1	hydrogel					75:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel	0:82	Minocycline-loaded cellulose nano whiskers/poly(sodium acrylate) composite hydrogel films as wound dressing.
25940526	1	69	theme	physiological	300:312	arg1	solution					321:328	physiological buffer solution	300:328	physiological buffer solution (PBS)	300:334	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	1	69	theme	physiological	300:312	arg1	PBS					331:333	PBS	331:333	PBS	331:333	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	2	70	theme	sodium	362:367	arg1	acrylate					369:376	sodium acrylate	362:376	sodium acrylate	362:376	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	2	70	theme	sodium	362:367	arg1	CNWs					351:354	CNWs	351:354	CNWs	351:354	The (CNWs)/poly(sodium acrylate) film, containing 9.7% (w/w) of CNWs, demonstrated Mic release of 2500 μg/g while the plain poly(acrylate) film showed 3100 μg/g of drug release.
25940526	1	71	theme	Minocycline	139:149	arg1	films					226:230	antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films	123:230	antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films	123:230	In this work, antibiotic drug Minocycline (Mic) loaded cellulose nano-whiskers (CNWs)/poly(sodium acrylate) hydrogel films were prepared and investigated for their drug releasing capacity in physiological buffer solution (PBS) at 37 °C.
25940526	9	72	theme	Mic-loaded	1455:1464	arg1	films					1466:1470	the Mic-loaded films	1451:1470	the Mic-loaded films	1451:1470	Finally, the Mic-loaded films showed fair anti-fungal and antibacterial properties.
25940526	3	73	theme	drug	639:642	arg1	release					644:650	the drug release	635:650	the drug release from the resulting films	635:675	In addition, with the increase in the concentration of cross-linker N,N'-methylene bisacrylamide (MB) from to, the drug release from the resulting films decreased from 507 to 191 μg/g.
25838353	6	0	dep	in	1335:1336	arg1	vitro					1338:1342	vitro	1338:1342	vitro	1338:1342	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	7	1	theme	therapeutic	1568:1578	arg1	RNAs					1598:1601	therapeutic small interfering RNAs	1568:1601	therapeutic small interfering RNAs	1568:1601	In conclusion, the results indicate that cationic liposome could be further developed as a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs.
25838353	5	2	with	surface	1073:1079	arg1	RNA					1132:1134	small interfering RNA	1114:1134	small interfering RNA	1114:1134	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	2	3	theme	paclitaxel	367:376	arg1	effect					357:362	the combined antitumor effect	334:362	the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan	334:499	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	6	4	theme	great	1254:1258	arg1	enhancement					1260:1270	a great enhancement	1252:1270	a great enhancement	1252:1270	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	5	5	with	ability	1101:1107	arg1	RNA					1132:1134	small interfering RNA	1114:1134	small interfering RNA	1114:1134	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	6	6	theme	higher	1328:1333	arg1	cytotoxicity					1344:1355	a significantly higher in vitro cytotoxicity	1312:1355	a significantly higher in vitro cytotoxicity in Bcap-37 cells	1312:1372	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	3	7	theme	-carbamoyl	574:583	arg1	-cholesterol					585:596	3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol	540:596	3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol	540:596	The cationic liposome was composed of 3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol, dioleoylphosphatidylethanolamine, and octadecyl quaternized carboxymethyl chitosan.
25838353	3	7	theme	-carbamoyl	574:583	arg1	N'-dimethylaminoethane					551:572	N'-dimethylaminoethane	551:572	N'-dimethylaminoethane	551:572	The cationic liposome was composed of 3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol, dioleoylphosphatidylethanolamine, and octadecyl quaternized carboxymethyl chitosan.
25838353	7	8	theme	codelivery	1523:1532	arg1	system					1534:1539	a codelivery system	1521:1539	a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs	1521:1601	In conclusion, the results indicate that cationic liposome could be further developed as a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs.
25838353	7	8	theme	codelivery	1523:1532	arg1	liposome					1482:1489	cationic liposome	1473:1489	cationic liposome	1473:1489	In conclusion, the results indicate that cationic liposome could be further developed as a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs.
25838353	6	9	theme	cationic	1226:1233	arg1	liposome					1235:1242	the composite cationic liposome	1212:1242	the composite cationic liposome	1212:1242	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	1	10	theme	cancer	217:222	arg1	resistance					229:238	cancer cell resistance	217:238	cancer cell resistance	217:238	Conventional therapeutic approaches for cancer are limited by cancer cell resistance, which has impeded their clinical applications.
25838353	6	11	theme	interfering	1409:1419	arg1	RNA					1421:1423	small interfering RNA	1403:1423	small interfering RNA alone	1403:1429	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	4	12	theme	zeta	808:811	arg1	potential					813:821	zeta potential	808:821	zeta potential	808:821	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	5	13	with	shape	1057:1061	arg1	RNA					1132:1134	small interfering RNA	1114:1134	small interfering RNA	1114:1134	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	6	14	theme	liposomal	1379:1387	arg1	paclitaxel					1389:1398	liposomal paclitaxel	1379:1398	liposomal paclitaxel	1379:1398	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	3	15	theme	quaternized	647:657	arg1	chitosan					673:680	octadecyl quaternized carboxymethyl chitosan	637:680	octadecyl quaternized carboxymethyl chitosan	637:680	The cationic liposome was composed of 3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol, dioleoylphosphatidylethanolamine, and octadecyl quaternized carboxymethyl chitosan.
25838353	5	16	theme	narrow	1005:1010	arg1	distribution					1026:1037	a narrow particle size distribution	1003:1037	a narrow particle size distribution	1003:1037	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	6	17	theme	antitumor	1279:1287	arg1	activity					1289:1296	the antitumor activity	1275:1296	the antitumor activity	1275:1296	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	5	18	theme	interfering	1120:1130	arg1	RNA					1132:1134	small interfering RNA	1114:1134	small interfering RNA	1114:1134	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	1	19	theme	Conventional	155:166	arg1	approaches					180:189	Conventional therapeutic approaches	155:189	Conventional therapeutic approaches for cancer	155:200	Conventional therapeutic approaches for cancer are limited by cancer cell resistance, which has impeded their clinical applications.
25838353	2	20	theme	modified	447:454	arg1	chitosan					492:499	modified octadecyl quaternized carboxymethyl chitosan	447:499	modified octadecyl quaternized carboxymethyl chitosan	447:499	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	4	21	theme	dynamic	779:785	arg1	scattering					793:802	dynamic light scattering	779:802	dynamic light scattering	779:802	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	0	22	theme	paclitaxel	14:23	arg1	Codelivery					0:9	Codelivery	0:9	Codelivery of paclitaxel and small interfering RNA by octadecyl	0:62	Codelivery of paclitaxel and small interfering RNA by octadecyl quaternized carboxymethyl chitosan-modified cationic liposome for combined cancer therapy.
25838353	5	23	theme	particle	988:995	arg1	size					997:1000	a small particle size	980:1000	a small particle size	980:1000	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	5	24	with	size	997:1000	arg1	RNA					1132:1134	small interfering RNA	1114:1134	small interfering RNA	1114:1134	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	4	25	theme	scattering	793:802	arg1	measurements					823:834	dynamic light scattering and zeta potential measurements	779:834	dynamic light scattering and zeta potential measurements	779:834	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	0	26	theme	carboxymethyl	76:88	arg1	liposome					117:124	carboxymethyl chitosan-modified cationic liposome	76:124	carboxymethyl chitosan-modified cationic liposome for combined cancer therapy	76:152	Codelivery of paclitaxel and small interfering RNA by octadecyl quaternized carboxymethyl chitosan-modified cationic liposome for combined cancer therapy.
25838353	4	27	theme	atomic	871:876	arg1	microscopy					884:893	atomic force microscopy	871:893	atomic force microscopy	871:893	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	0	28	theme	cationic	108:115	arg1	liposome					117:124	carboxymethyl chitosan-modified cationic liposome	76:124	carboxymethyl chitosan-modified cationic liposome for combined cancer therapy	76:152	Codelivery of paclitaxel and small interfering RNA by octadecyl quaternized carboxymethyl chitosan-modified cationic liposome for combined cancer therapy.
25838353	5	29	theme	size	1021:1024	arg1	distribution					1026:1037	a narrow particle size distribution	1003:1037	a narrow particle size distribution	1003:1037	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	2	30	theme	cationic	417:424	arg1	liposome					426:433	cationic liposome	417:433	cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan	417:499	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	4	31	theme	transmission	837:848	arg1	microscopy					859:868	transmission electron microscopy	837:868	transmission electron microscopy	837:868	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	2	32	theme	quaternized	466:476	arg1	chitosan					492:499	modified octadecyl quaternized carboxymethyl chitosan	447:499	modified octadecyl quaternized carboxymethyl chitosan	447:499	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	3	33	theme	cationic	506:513	arg1	liposome					515:522	The cationic liposome	502:522	The cationic liposome	502:522	The cationic liposome was composed of 3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol, dioleoylphosphatidylethanolamine, and octadecyl quaternized carboxymethyl chitosan.
25838353	5	34	theme	cationic	927:934	arg1	liposome					936:943	The cationic liposome	923:943	The cationic liposome	923:943	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	5	35	theme	good	1042:1045	arg1	shape					1057:1061	a good spherical shape	1040:1061	a good spherical shape	1040:1061	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	5	36	theme	small	982:986	arg1	size					997:1000	a small particle size	980:1000	a small particle size	980:1000	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	0	37	theme	small	29:33	arg1	RNA					47:49	small interfering RNA	29:49	small interfering RNA	29:49	Codelivery of paclitaxel and small interfering RNA by octadecyl quaternized carboxymethyl chitosan-modified cationic liposome for combined cancer therapy.
25838353	5	38	theme	good	1088:1091	arg1	ability					1101:1107	a good binding ability	1086:1107	a good binding ability	1086:1107	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	0	39	theme	RNA	47:49	arg1	Codelivery					0:9	Codelivery	0:9	Codelivery of paclitaxel and small interfering RNA by octadecyl	0:62	Codelivery of paclitaxel and small interfering RNA by octadecyl quaternized carboxymethyl chitosan-modified cationic liposome for combined cancer therapy.
25838353	6	40	theme	interfering	1195:1205	arg1	RNA					1207:1209	small interfering RNA	1189:1209	small interfering RNA	1189:1209	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	2	41	theme	antitumor	347:355	arg1	effect					357:362	the combined antitumor effect	334:362	the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan	334:499	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	6	42	from	cytotoxicity	1344:1355	arg1	cells					1368:1372	Bcap-37 cells	1360:1372	Bcap-37 cells	1360:1372	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	7	43	theme	small	1580:1584	arg1	RNAs					1598:1601	therapeutic small interfering RNAs	1568:1601	therapeutic small interfering RNAs	1568:1601	In conclusion, the results indicate that cationic liposome could be further developed as a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs.
25838353	4	44	theme	retardation	904:914	arg1	assay					916:920	gel retardation assay	900:920	gel retardation assay	900:920	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	2	45	theme	interfering	389:399	arg1	RNA					401:403	small interfering RNA	383:403	small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan	383:499	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	4	46	dep	Fourier	738:744	arg1	transform					746:754	transform	746:754	transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay	746:920	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	4	47	theme	cationic	687:694	arg1	properties					705:714	The cationic liposome properties	683:714	The cationic liposome properties	683:714	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	6	48	theme	Bcap-37	1360:1366	arg1	cells					1368:1372	Bcap-37 cells	1360:1372	Bcap-37 cells	1360:1372	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	6	49	dep	higher	1328:1333	arg1	in					1335:1336	in	1335:1336	in	1335:1336	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	7	50	theme	chemotherapy	1545:1556	arg1	drugs					1558:1562	chemotherapy drugs	1545:1562	chemotherapy drugs	1545:1562	In conclusion, the results indicate that cationic liposome could be further developed as a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs.
25838353	2	51	theme	work	310:313	arg1	goal					297:300	The main goal	288:300	The main goal of this work	288:313	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	6	52	theme	composite	1216:1224	arg1	liposome					1235:1242	the composite cationic liposome	1212:1242	the composite cationic liposome	1212:1242	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	5	53	with	distribution	1026:1037	arg1	RNA					1132:1134	small interfering RNA	1114:1134	small interfering RNA	1114:1134	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	1	54	theme	cell	224:227	arg1	resistance					229:238	cancer cell resistance	217:238	cancer cell resistance	217:238	Conventional therapeutic approaches for cancer are limited by cancer cell resistance, which has impeded their clinical applications.
25838353	7	55	theme	cationic	1473:1480	arg1	liposome					1482:1489	cationic liposome	1473:1489	cationic liposome	1473:1489	In conclusion, the results indicate that cationic liposome could be further developed as a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs.
25838353	7	55	theme	cationic	1473:1480	arg1	system					1534:1539	a codelivery system	1521:1539	a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs	1521:1601	In conclusion, the results indicate that cationic liposome could be further developed as a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs.
25838353	6	56	theme	small	1403:1407	arg1	RNA					1421:1423	small interfering RNA	1403:1423	small interfering RNA alone	1403:1429	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	3	57	theme	carboxymethyl	659:671	arg1	chitosan					673:680	octadecyl quaternized carboxymethyl chitosan	637:680	octadecyl quaternized carboxymethyl chitosan	637:680	The cationic liposome was composed of 3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol, dioleoylphosphatidylethanolamine, and octadecyl quaternized carboxymethyl chitosan.
25838353	5	58	theme	good	955:958	arg1	shape					1057:1061	a good spherical shape	1040:1061	a good spherical shape	1040:1061	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	5	58	theme	good	955:958	arg1	properties					960:969	good properties	955:969	good properties	955:969	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	5	58	theme	good	955:958	arg1	size					997:1000	a small particle size	980:1000	a small particle size	980:1000	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	5	58	theme	good	955:958	arg1	distribution					1026:1037	a narrow particle size distribution	1003:1037	a narrow particle size distribution	1003:1037	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	5	58	theme	good	955:958	arg1	ability					1101:1107	a good binding ability	1086:1107	a good binding ability	1086:1107	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	5	58	theme	good	955:958	arg1	surface					1073:1079	a smooth surface	1064:1079	a smooth surface	1064:1079	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	3	59	theme	octadecyl	637:645	arg1	chitosan					673:680	octadecyl quaternized carboxymethyl chitosan	637:680	octadecyl quaternized carboxymethyl chitosan	637:680	The cationic liposome was composed of 3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol, dioleoylphosphatidylethanolamine, and octadecyl quaternized carboxymethyl chitosan.
25838353	5	60	theme	small	1114:1118	arg1	RNA					1132:1134	small interfering RNA	1114:1134	small interfering RNA	1114:1134	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	2	61	theme	main	292:295	arg1	goal					297:300	The main goal	288:300	The main goal of this work	288:313	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	5	62	theme	particle	1012:1019	arg1	distribution					1026:1037	a narrow particle size distribution	1003:1037	a narrow particle size distribution	1003:1037	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	4	63	theme	potential	813:821	arg1	measurements					823:834	dynamic light scattering and zeta potential measurements	779:834	dynamic light scattering and zeta potential measurements	779:834	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	1	64	theme	therapeutic	168:178	arg1	approaches					180:189	Conventional therapeutic approaches	155:189	Conventional therapeutic approaches for cancer	155:200	Conventional therapeutic approaches for cancer are limited by cancer cell resistance, which has impeded their clinical applications.
25838353	0	65	theme	chitosan-modified	90:106	arg1	liposome					117:124	carboxymethyl chitosan-modified cationic liposome	76:124	carboxymethyl chitosan-modified cationic liposome for combined cancer therapy	76:152	Codelivery of paclitaxel and small interfering RNA by octadecyl quaternized carboxymethyl chitosan-modified cationic liposome for combined cancer therapy.
25838353	0	66	theme	cancer	139:144	arg1	therapy					146:152	combined cancer therapy	130:152	combined cancer therapy	130:152	Codelivery of paclitaxel and small interfering RNA by octadecyl quaternized carboxymethyl chitosan-modified cationic liposome for combined cancer therapy.
25838353	4	67	theme	light	787:791	arg1	scattering					793:802	dynamic light scattering	779:802	dynamic light scattering	779:802	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	5	68	theme	spherical	1047:1055	arg1	shape					1057:1061	a good spherical shape	1040:1061	a good spherical shape	1040:1061	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	0	69	theme	combined	130:137	arg1	therapy					146:152	combined cancer therapy	130:152	combined cancer therapy	130:152	Codelivery of paclitaxel and small interfering RNA by octadecyl quaternized carboxymethyl chitosan-modified cationic liposome for combined cancer therapy.
25838353	4	70	theme	force	878:882	arg1	microscopy					884:893	atomic force microscopy	871:893	atomic force microscopy	871:893	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	2	71	theme	carboxymethyl	478:490	arg1	chitosan					492:499	modified octadecyl quaternized carboxymethyl chitosan	447:499	modified octadecyl quaternized carboxymethyl chitosan	447:499	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	2	72	theme	octadecyl	456:464	arg1	chitosan					492:499	modified octadecyl quaternized carboxymethyl chitosan	447:499	modified octadecyl quaternized carboxymethyl chitosan	447:499	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	4	73	theme	electron	850:857	arg1	microscopy					859:868	transmission electron microscopy	837:868	transmission electron microscopy	837:868	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	0	74	theme	interfering	35:45	arg1	RNA					47:49	small interfering RNA	29:49	small interfering RNA	29:49	Codelivery of paclitaxel and small interfering RNA by octadecyl quaternized carboxymethyl chitosan-modified cationic liposome for combined cancer therapy.
25838353	2	75	with	effect	357:362	arg1	RNA					401:403	small interfering RNA	383:403	small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan	383:499	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	5	76	theme	binding	1093:1099	arg1	ability					1101:1107	a good binding ability	1086:1107	a good binding ability	1086:1107	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	3	77	theme	3β-[N-	540:545	arg1	-cholesterol					585:596	3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol	540:596	3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol	540:596	The cationic liposome was composed of 3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol, dioleoylphosphatidylethanolamine, and octadecyl quaternized carboxymethyl chitosan.
25838353	3	77	theme	3β-[N-	540:545	arg1	N'-dimethylaminoethane					551:572	N'-dimethylaminoethane	551:572	N'-dimethylaminoethane	551:572	The cationic liposome was composed of 3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol, dioleoylphosphatidylethanolamine, and octadecyl quaternized carboxymethyl chitosan.
25838353	5	78	theme	smooth	1066:1071	arg1	surface					1073:1079	a smooth surface	1064:1079	a smooth surface	1064:1079	The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA.
25838353	6	79	theme	small	1189:1193	arg1	RNA					1207:1209	small interfering RNA	1189:1209	small interfering RNA	1189:1209	Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone.
25838353	2	80	theme	combined	338:345	arg1	effect					357:362	the combined antitumor effect	334:362	the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan	334:499	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	7	81	theme	interfering	1586:1596	arg1	RNAs					1598:1601	therapeutic small interfering RNAs	1568:1601	therapeutic small interfering RNAs	1568:1601	In conclusion, the results indicate that cationic liposome could be further developed as a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs.
25838353	4	82	dep	transform	746:754	arg1	infrared					756:763	infrared	756:763	transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay	746:920	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	4	83	theme	liposome	696:703	arg1	properties					705:714	The cationic liposome properties	683:714	The cationic liposome properties	683:714	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	4	84	theme	gel	900:902	arg1	assay					916:920	gel retardation assay	900:920	gel retardation assay	900:920	The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay.
25838353	2	85	theme	small	383:387	arg1	RNA					401:403	small interfering RNA	383:403	small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan	383:499	The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan.
25838353	1	86	theme	clinical	265:272	arg1	applications					274:285	their clinical applications	259:285	their clinical applications	259:285	Conventional therapeutic approaches for cancer are limited by cancer cell resistance, which has impeded their clinical applications.
25707750	0	0	theme	gellan	64:69	arg1	gum					71:73	gellan gum	64:73	gellan gum for ophthalmic delivery of curcumin	64:109	Novel in situ gel systems based on P123/TPGS mixed micelles and gellan gum for ophthalmic delivery of curcumin.
25707750	6	1	theme	gellan	933:938	arg1	solution					944:951	gellan gum solution	933:951	gellan gum solution (0.2%, w/w)	933:963	After dispersing the micelles in gellan gum solution (0.2%, w/w) at the ratio of 3:1 and 1:1 (v/v), respectively, CUR-MM-ISGs were formed and presented transparent appearance.
25707750	1	2	theme	natural	124:130	arg1	compound					143:150	a natural polyphenol compound	122:150	a natural polyphenol compound	122:150	Curcumin, a natural polyphenol compound, has been widely reported for diverse pharmacological effects and already been investigated for eye diseases.
25707750	1	2	theme	natural	124:130	arg1	Curcumin					112:119	Curcumin	112:119	Curcumin	112:119	Curcumin, a natural polyphenol compound, has been widely reported for diverse pharmacological effects and already been investigated for eye diseases.
25707750	6	3	theme	3:1	981:983	arg1	ratio					972:976	the ratio	968:976	the ratio of 3:1 and 1:1 (v/v)	968:997	After dispersing the micelles in gellan gum solution (0.2%, w/w) at the ratio of 3:1 and 1:1 (v/v), respectively, CUR-MM-ISGs were formed and presented transparent appearance.
25707750	5	4	theme	Characterization	777:792	arg1	tests					794:798	Characterization tests	777:798	Characterization tests	777:798	Characterization tests showed that CUR-MMs were in spherical shape with small size and low critical micelle concentration.
25707750	7	5	theme	release	1086:1092	arg1	profile					1094:1100	Sustained release profile	1076:1100	Sustained release profile	1076:1100	Sustained release profile was obtained in vitro for both CUR-MM-ISGs (3:1 or 1:1, v/v).
25707750	10	6	theme	gel	1612:1614	arg1	carrier					1658:1664	a potential ophthalmic delivery carrier	1626:1664	a potential ophthalmic delivery carrier for curcumin	1626:1677	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	10	6	theme	gel	1612:1614	arg1	system					1616:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	1	7	theme	polyphenol	132:141	arg1	compound					143:150	a natural polyphenol compound	122:150	a natural polyphenol compound	122:150	Curcumin, a natural polyphenol compound, has been widely reported for diverse pharmacological effects and already been investigated for eye diseases.
25707750	1	7	theme	polyphenol	132:141	arg1	Curcumin					112:119	Curcumin	112:119	Curcumin	112:119	Curcumin, a natural polyphenol compound, has been widely reported for diverse pharmacological effects and already been investigated for eye diseases.
25707750	9	8	theme	permeation	1375:1384	arg1	3:1					1409:1411	3:1	1409:1411	3:1	1409:1411	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	8	theme	permeation	1375:1384	arg1	CUR-MM-ISGs					1396:1406	CUR-MM-ISGs	1396:1406	CUR-MM-ISGs (3:1, v/v)	1396:1417	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	8	theme	permeation	1375:1384	arg1	amount					1386:1391	the cumulative drug permeation amount	1355:1391	the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v)	1355:1417	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	8	theme	permeation	1375:1384	arg1	1.16-fold					1436:1444	1.16-fold	1436:1444	1.16-fold	1436:1444	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	6	9	theme	transparent	1052:1062	arg1	appearance					1064:1073	transparent appearance	1052:1073	transparent appearance	1052:1073	After dispersing the micelles in gellan gum solution (0.2%, w/w) at the ratio of 3:1 and 1:1 (v/v), respectively, CUR-MM-ISGs were formed and presented transparent appearance.
25707750	7	10	theme	Sustained	1076:1084	arg1	profile					1094:1100	Sustained release profile	1076:1100	Sustained release profile	1076:1100	Sustained release profile was obtained in vitro for both CUR-MM-ISGs (3:1 or 1:1, v/v).
25707750	10	11	theme	soluble	1691:1697	arg1	drug					1699:1702	a poorly soluble drug	1682:1702	a poorly soluble drug	1682:1702	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	4	12	theme	micelles	757:764	arg1	optimization					709:720	the optimization	705:720	the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs)	705:774	Central composite design-response surface methodology was applied for the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs).
25707750	10	13	theme	potential	1628:1636	arg1	carrier					1658:1664	a potential ophthalmic delivery carrier	1626:1664	a potential ophthalmic delivery carrier for curcumin	1626:1677	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	10	13	theme	potential	1628:1636	arg1	system					1616:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	4	14	theme	mixed	751:755	arg1	micelles					757:764	curcumin-loaded P123/TPGS mixed micelles	725:764	curcumin-loaded P123/TPGS mixed micelles (CUR-MMs)	725:774	Central composite design-response surface methodology was applied for the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs).
25707750	4	14	theme	mixed	751:755	arg1	CUR-MMs					767:773	CUR-MMs	767:773	CUR-MMs	767:773	Central composite design-response surface methodology was applied for the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs).
25707750	3	15	theme	micelle	534:540	arg1	CUR-MM-ISGs					556:566	CUR-MM-ISGs	556:566	CUR-MM-ISGs	556:566	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	3	15	theme	micelle	534:540	arg1	gels					550:553	micelle in situ gels	534:553	micelle in situ gels (CUR-MM-ISGs)	534:567	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	10	16	theme	ion-sensitive	1576:1588	arg1	carrier					1658:1664	a potential ophthalmic delivery carrier	1626:1664	a potential ophthalmic delivery carrier for curcumin	1626:1677	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	10	16	theme	ion-sensitive	1576:1588	arg1	system					1616:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	3	17	theme	/D-a-tocopheryl	476:490	arg1	TPGS					522:525	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS)	426:526	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability	426:632	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	1	18	theme	eye	248:250	arg1	diseases					252:259	eye diseases	248:259	eye diseases	248:259	Curcumin, a natural polyphenol compound, has been widely reported for diverse pharmacological effects and already been investigated for eye diseases.
25707750	2	19	theme	penetration	323:333	arg1	barriers					335:342	the inherent penetration barriers	310:342	the inherent penetration barriers in cornea	310:352	However, the water-insolubility of curcumin and the inherent penetration barriers in cornea make it difficult for curcumin to enter eye.
25707750	5	20	theme	small	849:853	arg1	size					855:858	small size	849:858	small size	849:858	Characterization tests showed that CUR-MMs were in spherical shape with small size and low critical micelle concentration.
25707750	8	21	theme	ophthalmic	1211:1220	arg1	formulations					1222:1233	ophthalmic formulations	1211:1233	ophthalmic formulations	1211:1233	The irritation test proved that CUR-MM-ISGs as ophthalmic formulations were gentle and biocompatible towards ocular tissues.
25707750	5	22	theme	micelle	877:883	arg1	concentration					885:897	low critical micelle concentration	864:897	low critical micelle concentration	864:897	Characterization tests showed that CUR-MMs were in spherical shape with small size and low critical micelle concentration.
25707750	4	23	theme	composite	643:651	arg1	methodology					677:687	Central composite design-response surface methodology	635:687	Central composite design-response surface methodology	635:687	Central composite design-response surface methodology was applied for the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs).
25707750	0	24	theme	ophthalmic	79:88	arg1	delivery					90:97	ophthalmic delivery	79:97	ophthalmic delivery of curcumin	79:109	Novel in situ gel systems based on P123/TPGS mixed micelles and gellan gum for ophthalmic delivery of curcumin.
25707750	9	25	theme	drug	1370:1373	arg1	3:1					1409:1411	3:1	1409:1411	3:1	1409:1411	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	25	theme	drug	1370:1373	arg1	CUR-MM-ISGs					1396:1406	CUR-MM-ISGs	1396:1406	CUR-MM-ISGs (3:1, v/v)	1396:1417	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	25	theme	drug	1370:1373	arg1	amount					1386:1391	the cumulative drug permeation amount	1355:1391	the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v)	1355:1417	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	25	theme	drug	1370:1373	arg1	1.16-fold					1436:1444	1.16-fold	1436:1444	1.16-fold	1436:1444	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	4	26	theme	Central	635:641	arg1	methodology					677:687	Central composite design-response surface methodology	635:687	Central composite design-response surface methodology	635:687	Central composite design-response surface methodology was applied for the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs).
25707750	0	27	theme	in	6:7	arg1	systems					18:24	Novel in situ gel systems	0:24	Novel in situ gel systems	0:24	Novel in situ gel systems based on P123/TPGS mixed micelles and gellan gum for ophthalmic delivery of curcumin.
25707750	2	28	from	barriers	335:342	arg1	cornea					347:352	cornea	347:352	cornea	347:352	However, the water-insolubility of curcumin and the inherent penetration barriers in cornea make it difficult for curcumin to enter eye.
25707750	2	29	theme	curcumin	297:304	arg1	water-insolubility					275:292	the water-insolubility	271:292	the water-insolubility of curcumin	271:304	However, the water-insolubility of curcumin and the inherent penetration barriers in cornea make it difficult for curcumin to enter eye.
25707750	2	29	theme	curcumin	297:304	arg1	barriers					335:342	the inherent penetration barriers	310:342	the inherent penetration barriers in cornea	310:352	However, the water-insolubility of curcumin and the inherent penetration barriers in cornea make it difficult for curcumin to enter eye.
25707750	4	30	theme	surface	669:675	arg1	methodology					677:687	Central composite design-response surface methodology	635:687	Central composite design-response surface methodology	635:687	Central composite design-response surface methodology was applied for the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs).
25707750	0	31	theme	Novel	0:4	arg1	systems					18:24	Novel in situ gel systems	0:24	Novel in situ gel systems	0:24	Novel in situ gel systems based on P123/TPGS mixed micelles and gellan gum for ophthalmic delivery of curcumin.
25707750	9	32	dep	CUR-MM-ISGs	1472:1482	arg1	solution					1508:1515	solution	1508:1515	solution	1508:1515	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	32	dep	CUR-MM-ISGs	1472:1482	arg1	v/v					1490:1492	v/v	1490:1492	v/v	1490:1492	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	32	dep	CUR-MM-ISGs	1472:1482	arg1	1:1					1485:1487	1:1	1485:1487	1:1	1485:1487	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	4	33	theme	P123/TPGS	741:749	arg1	micelles					757:764	curcumin-loaded P123/TPGS mixed micelles	725:764	curcumin-loaded P123/TPGS mixed micelles (CUR-MMs)	725:774	Central composite design-response surface methodology was applied for the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs).
25707750	4	33	theme	P123/TPGS	741:749	arg1	CUR-MMs					767:773	CUR-MMs	767:773	CUR-MMs	767:773	Central composite design-response surface methodology was applied for the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs).
25707750	4	34	theme	design-response	653:667	arg1	methodology					677:687	Central composite design-response surface methodology	635:687	Central composite design-response surface methodology	635:687	Central composite design-response surface methodology was applied for the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs).
25707750	0	35	theme	gel	14:16	arg1	systems					18:24	Novel in situ gel systems	0:24	Novel in situ gel systems	0:24	Novel in situ gel systems based on P123/TPGS mixed micelles and gellan gum for ophthalmic delivery of curcumin.
25707750	3	36	theme	ocular	580:585	arg1	time					597:600	ocular retention time	580:600	ocular retention time	580:600	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	0	37	theme	curcumin	102:109	arg1	delivery					90:97	ophthalmic delivery	79:97	ophthalmic delivery of curcumin	79:109	Novel in situ gel systems based on P123/TPGS mixed micelles and gellan gum for ophthalmic delivery of curcumin.
25707750	9	38	theme	CUR-MM-ISGs	1396:1406	arg1	3:1					1409:1411	3:1	1409:1411	3:1	1409:1411	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	38	theme	CUR-MM-ISGs	1396:1406	arg1	CUR-MM-ISGs					1396:1406	CUR-MM-ISGs	1396:1406	CUR-MM-ISGs (3:1, v/v)	1396:1417	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	38	theme	CUR-MM-ISGs	1396:1406	arg1	amount					1386:1391	the cumulative drug permeation amount	1355:1391	the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v)	1355:1417	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	38	theme	CUR-MM-ISGs	1396:1406	arg1	1.16-fold					1436:1444	1.16-fold	1436:1444	1.16-fold	1436:1444	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	3	39	theme	in	542:543	arg1	CUR-MM-ISGs					556:566	CUR-MM-ISGs	556:566	CUR-MM-ISGs	556:566	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	3	39	theme	in	542:543	arg1	gels					550:553	micelle in situ gels	534:553	micelle in situ gels (CUR-MM-ISGs)	534:567	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	9	40	dep	3:1	1409:1411	arg1	v/v					1414:1416	v/v	1414:1416	v/v	1414:1416	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	3	41	theme	curcumin-loaded	440:454	arg1	TPGS					522:525	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS)	426:526	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability	426:632	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	5	42	with	shape	838:842	arg1	size					855:858	small size	849:858	small size	849:858	Characterization tests showed that CUR-MMs were in spherical shape with small size and low critical micelle concentration.
25707750	5	42	with	shape	838:842	arg1	concentration					885:897	low critical micelle concentration	864:897	low critical micelle concentration	864:897	Characterization tests showed that CUR-MMs were in spherical shape with small size and low critical micelle concentration.
25707750	10	43	theme	mixed	1590:1594	arg1	carrier					1658:1664	a potential ophthalmic delivery carrier	1626:1664	a potential ophthalmic delivery carrier for curcumin	1626:1677	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	10	43	theme	mixed	1590:1594	arg1	system					1616:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	3	44	theme	polyethylene	492:503	arg1	TPGS					522:525	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS)	426:526	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability	426:632	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	3	45	theme	Pluronic	456:463	arg1	TPGS					522:525	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS)	426:526	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability	426:632	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	9	46	theme	ex	1306:1307	arg1	study					1334:1338	the ex vivo corneal penetration study	1302:1338	the ex vivo corneal penetration study	1302:1338	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	47	dep	ex	1306:1307	arg1	vivo					1309:1312	vivo	1309:1312	vivo	1309:1312	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	6	48	dep	solution	944:951	arg1	w/w					960:962	w/w	960:962	w/w	960:962	After dispersing the micelles in gellan gum solution (0.2%, w/w) at the ratio of 3:1 and 1:1 (v/v), respectively, CUR-MM-ISGs were formed and presented transparent appearance.
25707750	6	48	dep	solution	944:951	arg1	%					957:957	0.2%	954:957	0.2%	954:957	After dispersing the micelles in gellan gum solution (0.2%, w/w) at the ratio of 3:1 and 1:1 (v/v), respectively, CUR-MM-ISGs were formed and presented transparent appearance.
25707750	2	49	dep	enter	388:392	arg1	curcumin					376:383	curcumin	376:383	curcumin	376:383	However, the water-insolubility of curcumin and the inherent penetration barriers in cornea make it difficult for curcumin to enter eye.
25707750	2	49	dep	enter	388:392	arg1	difficult					362:370	difficult	362:370	difficult	362:370	However, the water-insolubility of curcumin and the inherent penetration barriers in cornea make it difficult for curcumin to enter eye.
25707750	10	50	theme	developed	1566:1574	arg1	carrier					1658:1664	a potential ophthalmic delivery carrier	1626:1664	a potential ophthalmic delivery carrier for curcumin	1626:1677	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	10	50	theme	developed	1566:1574	arg1	system					1616:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	5	51	theme	low	864:866	arg1	concentration					885:897	low critical micelle concentration	864:897	low critical micelle concentration	864:897	Characterization tests showed that CUR-MMs were in spherical shape with small size and low critical micelle concentration.
25707750	10	52	theme	micelle	1596:1602	arg1	carrier					1658:1664	a potential ophthalmic delivery carrier	1626:1664	a potential ophthalmic delivery carrier for curcumin	1626:1677	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	10	52	theme	micelle	1596:1602	arg1	system					1616:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	5	53	theme	spherical	828:836	arg1	shape					838:842	spherical shape	828:842	spherical shape	828:842	Characterization tests showed that CUR-MMs were in spherical shape with small size and low critical micelle concentration.
25707750	1	54	theme	diverse	182:188	arg1	effects					206:212	diverse pharmacological effects	182:212	diverse pharmacological effects	182:212	Curcumin, a natural polyphenol compound, has been widely reported for diverse pharmacological effects and already been investigated for eye diseases.
25707750	0	55	theme	P123/TPGS	35:43	arg1	micelles					51:58	P123/TPGS mixed micelles	35:58	P123/TPGS mixed micelles	35:58	Novel in situ gel systems based on P123/TPGS mixed micelles and gellan gum for ophthalmic delivery of curcumin.
25707750	9	56	theme	corneal	1314:1320	arg1	study					1334:1338	the ex vivo corneal penetration study	1302:1338	the ex vivo corneal penetration study	1302:1338	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	3	57	theme	cornea	614:619	arg1	permeability					621:632	cornea permeability	614:632	cornea permeability	614:632	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	10	58	theme	ophthalmic	1638:1647	arg1	carrier					1658:1664	a potential ophthalmic delivery carrier	1626:1664	a potential ophthalmic delivery carrier for curcumin	1626:1677	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	10	58	theme	ophthalmic	1638:1647	arg1	system					1616:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	10	59	theme	delivery	1649:1656	arg1	carrier					1658:1664	a potential ophthalmic delivery carrier	1626:1664	a potential ophthalmic delivery carrier for curcumin	1626:1677	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	10	59	theme	delivery	1649:1656	arg1	system					1616:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	3	60	theme	P123	471:474	arg1	TPGS					522:525	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS)	426:526	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability	426:632	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	9	61	theme	penetration	1322:1332	arg1	study					1334:1338	the ex vivo corneal penetration study	1302:1338	the ex vivo corneal penetration study	1302:1338	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	2	62	theme	inherent	314:321	arg1	barriers					335:342	the inherent penetration barriers	310:342	the inherent penetration barriers in cornea	310:352	However, the water-insolubility of curcumin and the inherent penetration barriers in cornea make it difficult for curcumin to enter eye.
25707750	6	63	from	micelles	921:928	arg1	solution					944:951	gellan gum solution	933:951	gellan gum solution (0.2%, w/w)	933:963	After dispersing the micelles in gellan gum solution (0.2%, w/w) at the ratio of 3:1 and 1:1 (v/v), respectively, CUR-MM-ISGs were formed and presented transparent appearance.
25707750	7	64	dep	3:1	1146:1148	arg1	v/v					1158:1160	v/v	1158:1160	v/v	1158:1160	Sustained release profile was obtained in vitro for both CUR-MM-ISGs (3:1 or 1:1, v/v).
25707750	10	65	dep	in	1604:1605	arg1	situ					1607:1610	situ	1607:1610	situ	1607:1610	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	3	66	theme	P123	465:468	arg1	TPGS					522:525	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS)	426:526	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability	426:632	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	1	67	theme	pharmacological	190:204	arg1	effects					206:212	diverse pharmacological effects	182:212	diverse pharmacological effects	182:212	Curcumin, a natural polyphenol compound, has been widely reported for diverse pharmacological effects and already been investigated for eye diseases.
25707750	10	68	theme	in	1604:1605	arg1	carrier					1658:1664	a potential ophthalmic delivery carrier	1626:1664	a potential ophthalmic delivery carrier for curcumin	1626:1677	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	10	68	theme	in	1604:1605	arg1	system					1616:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	the developed ion-sensitive mixed micelle in situ gel system	1562:1621	It can be concluded from these results that the developed ion-sensitive mixed micelle in situ gel system is a potential ophthalmic delivery carrier for curcumin as a poorly soluble drug.
25707750	8	69	theme	irritation	1168:1177	arg1	test					1179:1182	The irritation test	1164:1182	The irritation test	1164:1182	The irritation test proved that CUR-MM-ISGs as ophthalmic formulations were gentle and biocompatible towards ocular tissues.
25707750	4	70	theme	curcumin-loaded	725:739	arg1	micelles					757:764	curcumin-loaded P123/TPGS mixed micelles	725:764	curcumin-loaded P123/TPGS mixed micelles (CUR-MMs)	725:774	Central composite design-response surface methodology was applied for the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs).
25707750	4	70	theme	curcumin-loaded	725:739	arg1	CUR-MMs					767:773	CUR-MMs	767:773	CUR-MMs	767:773	Central composite design-response surface methodology was applied for the optimization of curcumin-loaded P123/TPGS mixed micelles (CUR-MMs).
25707750	3	71	theme	ion-sensitive	426:438	arg1	TPGS					522:525	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS)	426:526	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability	426:632	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	0	72	dep	in	6:7	arg1	situ					9:12	situ	9:12	situ	9:12	Novel in situ gel systems based on P123/TPGS mixed micelles and gellan gum for ophthalmic delivery of curcumin.
25707750	3	73	dep	in	542:543	arg1	situ					545:548	situ	545:548	situ	545:548	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	3	74	theme	glycolsuccinate	505:519	arg1	TPGS					522:525	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS)	426:526	ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability	426:632	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	5	75	theme	critical	868:875	arg1	concentration					885:897	low critical micelle concentration	864:897	low critical micelle concentration	864:897	Characterization tests showed that CUR-MMs were in spherical shape with small size and low critical micelle concentration.
25707750	6	76	theme	1:1	989:991	arg1	ratio					972:976	the ratio	968:976	the ratio of 3:1 and 1:1 (v/v)	968:997	After dispersing the micelles in gellan gum solution (0.2%, w/w) at the ratio of 3:1 and 1:1 (v/v), respectively, CUR-MM-ISGs were formed and presented transparent appearance.
25707750	0	77	theme	mixed	45:49	arg1	micelles					51:58	P123/TPGS mixed micelles	35:58	P123/TPGS mixed micelles	35:58	Novel in situ gel systems based on P123/TPGS mixed micelles and gellan gum for ophthalmic delivery of curcumin.
25707750	6	78	theme	gum	940:942	arg1	solution					944:951	gellan gum solution	933:951	gellan gum solution (0.2%, w/w)	933:963	After dispersing the micelles in gellan gum solution (0.2%, w/w) at the ratio of 3:1 and 1:1 (v/v), respectively, CUR-MM-ISGs were formed and presented transparent appearance.
25707750	3	79	theme	retention	587:595	arg1	time					597:600	ocular retention time	580:600	ocular retention time	580:600	This work aimed to develop ion-sensitive curcumin-loaded Pluronic P123 (P123)/D-a-tocopheryl polyethylene glycolsuccinate (TPGS) mixed micelle in situ gels (CUR-MM-ISGs) to prolong ocular retention time and improve cornea permeability.
25707750	8	80	theme	ocular	1273:1278	arg1	tissues					1280:1286	ocular tissues	1273:1286	ocular tissues	1273:1286	The irritation test proved that CUR-MM-ISGs as ophthalmic formulations were gentle and biocompatible towards ocular tissues.
25707750	2	81	from	water-insolubility	275:292	arg1	cornea					347:352	cornea	347:352	cornea	347:352	However, the water-insolubility of curcumin and the inherent penetration barriers in cornea make it difficult for curcumin to enter eye.
25707750	9	82	theme	cumulative	1359:1368	arg1	3:1					1409:1411	3:1	1409:1411	3:1	1409:1411	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	82	theme	cumulative	1359:1368	arg1	CUR-MM-ISGs					1396:1406	CUR-MM-ISGs	1396:1406	CUR-MM-ISGs (3:1, v/v)	1396:1417	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	82	theme	cumulative	1359:1368	arg1	amount					1386:1391	the cumulative drug permeation amount	1355:1391	the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v)	1355:1417	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
25707750	9	82	theme	cumulative	1359:1368	arg1	1.16-fold					1436:1444	1.16-fold	1436:1444	1.16-fold	1436:1444	In addition, the ex vivo corneal penetration study indicated that the cumulative drug permeation amount of CUR-MM-ISGs (3:1, v/v) was respectively 1.16-fold and 1.32-fold higher than CUR-MM-ISGs (1:1, v/v) and curcumin solution.
28325318	0	0	theme	engineering	115:125	arg1	applications					127:138	bone tissue engineering applications	103:138	bone tissue engineering applications	103:138	Preparation and characterization of amine functional nano-hydroxyapatite/chitosan bionanocomposite for bone tissue engineering applications.
28325318	1	1	theme	nano-hydroxyapatite/chitosan	347:374	arg1	scaffolds					401:409	nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds	347:409	nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds	347:409	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	3	2	theme	cytotoxicity	942:953	arg1	level					933:937	the least and acceptable level	908:937	the least and acceptable level of cytotoxicity	908:953	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	1	3	theme	scaffolds	181:189	arg1	types					172:176	three different types	156:176	three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2)	156:336	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	3	4	theme	most	855:858	arg1	proliferation					865:877	the most cell proliferation	851:877	the most cell proliferation	851:877	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	1	5	theme	nano-hydroxyapatite/chitosan	265:292	arg1	composite					294:302	nano-hydroxyapatite/chitosan composite	265:302	nano-hydroxyapatite/chitosan composite (CTS+nHAP)	265:313	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	1	5	theme	nano-hydroxyapatite/chitosan	265:292	arg1	CTS+nHAP					305:312	CTS+nHAP	305:312	CTS+nHAP	305:312	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	0	6	theme	tissue	108:113	arg1	applications					127:138	bone tissue engineering applications	103:138	bone tissue engineering applications	103:138	Preparation and characterization of amine functional nano-hydroxyapatite/chitosan bionanocomposite for bone tissue engineering applications.
28325318	1	7	theme	composite	376:384	arg1	scaffolds					401:409	nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds	347:409	nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds	347:409	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	2	8	theme	CTS+nHAP-NH2	639:650	arg1	scaffold					652:659	the CTS+nHAP-NH2 scaffold	635:659	the CTS+nHAP-NH2 scaffold	635:659	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	4	9	dep	scaffolds	1193:1201	arg1	whereas					1163:1169	whereas	1163:1169	whereas	1163:1169	Finally, during osteoinductivity assessment, CTS+nHAP-NH2 nearly tripled initial alkaline phosphatase (ALP) activity when whereas both CTS and CTS+nHAP scaffolds only doubled.
28325318	4	9	dep	scaffolds	1193:1201	arg1	doubled					1208:1214	doubled	1208:1214	scaffolds only doubled	1193:1214	Finally, during osteoinductivity assessment, CTS+nHAP-NH2 nearly tripled initial alkaline phosphatase (ALP) activity when whereas both CTS and CTS+nHAP scaffolds only doubled.
28325318	3	10	theme	mesenchymal	1006:1016	arg1	cells					1023:1027	human bone mesenchymal stem cells	995:1027	human bone mesenchymal stem cells (hBM-MSCs)	995:1038	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	3	10	theme	mesenchymal	1006:1016	arg1	hBM-MSCs					1030:1037	hBM-MSCs	1030:1037	hBM-MSCs	1030:1037	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	5	11	theme	applicable	1424:1433	arg1	scaffold					1386:1393	CTS+nHAP-NH2 scaffold	1373:1393	CTS+nHAP-NH2 scaffold	1373:1393	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	5	11	theme	applicable	1424:1433	arg1	one					1435:1437	the most promising and applicable one	1401:1437	the most promising and applicable one	1401:1437	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	4	12	theme	phosphatase	1131:1141	arg1	activity					1149:1156	initial alkaline phosphatase (ALP) activity	1114:1156	initial alkaline phosphatase (ALP) activity	1114:1156	Finally, during osteoinductivity assessment, CTS+nHAP-NH2 nearly tripled initial alkaline phosphatase (ALP) activity when whereas both CTS and CTS+nHAP scaffolds only doubled.
28325318	2	13	theme	scaffold	521:528	arg1	types					530:534	all scaffold types	517:534	all scaffold types	517:534	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	1	14	theme	CTS+nHAP-NH2	387:398	arg1	scaffolds					401:409	nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds	347:409	nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds	347:409	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	3	15	theme	stem	1018:1021	arg1	cells					1023:1027	human bone mesenchymal stem cells	995:1027	human bone mesenchymal stem cells (hBM-MSCs)	995:1038	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	3	15	theme	stem	1018:1021	arg1	hBM-MSCs					1030:1037	hBM-MSCs	1030:1037	hBM-MSCs	1030:1037	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	3	16	theme	dehydrogenase	966:978	arg1	test					986:989	lactate dehydrogenase (LDH) test	958:989	lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs)	958:1038	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	5	17	theme	certain	1301:1307	arg1	potential					1309:1317	certain potential	1301:1317	certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one	1301:1437	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	6	18	theme	osteogenic	1491:1500	arg1	differentiation					1502:1516	osteogenic differentiation	1491:1516	osteogenic differentiation	1491:1516	In the future, we plan to intensify our studies on osteogenic differentiation on our scaffolds on a detailed molecular level and to include in vivo studies for pre-clinical purposes.
28325318	6	19	theme	detailed	1540:1547	arg1	level					1559:1563	a detailed molecular level	1538:1563	a detailed molecular level	1538:1563	In the future, we plan to intensify our studies on osteogenic differentiation on our scaffolds on a detailed molecular level and to include in vivo studies for pre-clinical purposes.
28325318	2	20	theme	weight	621:626	arg1	%					602:602	up to 15%	594:602	up to 15% of their initial weight	594:626	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	2	20	theme	weight	621:626	arg1	weight					621:626	their initial weight	607:626	their initial weight	607:626	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	3	21	theme	WST-1	882:886	arg1	assay					888:892	WST-1 assay	882:892	WST-1 assay	882:892	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	2	22	dep	15	600:601	arg1	to					597:598	to	597:598	to	597:598	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	2	23	theme	study	492:496	arg1	it					499:500	it	499:500	it	499:500	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	2	23	theme	study	492:496	arg1	results					477:483	results	477:483	results of the study	477:496	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	3	24	theme	human	995:999	arg1	cells					1023:1027	human bone mesenchymal stem cells	995:1027	human bone mesenchymal stem cells (hBM-MSCs)	995:1038	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	3	24	theme	human	995:999	arg1	hBM-MSCs					1030:1037	hBM-MSCs	1030:1037	hBM-MSCs	1030:1037	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	3	25	theme	cell	860:863	arg1	proliferation					865:877	the most cell proliferation	851:877	the most cell proliferation	851:877	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	6	26	theme	pre-clinical	1600:1611	arg1	purposes					1613:1620	pre-clinical purposes	1600:1620	pre-clinical purposes	1600:1620	In the future, we plan to intensify our studies on osteogenic differentiation on our scaffolds on a detailed molecular level and to include in vivo studies for pre-clinical purposes.
28325318	3	27	theme	bone	1001:1004	arg1	cells					1023:1027	human bone mesenchymal stem cells	995:1027	human bone mesenchymal stem cells (hBM-MSCs)	995:1038	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	3	27	theme	bone	1001:1004	arg1	hBM-MSCs					1030:1037	hBM-MSCs	1030:1037	hBM-MSCs	1030:1037	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	5	28	contain	have	1296:1299	arg1	types					1270:1274	all synthesized scaffold types	1245:1274	all synthesized scaffold types under investigation	1245:1294	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	5	28	contain	have	1296:1299	arg2	potential					1309:1317	certain potential	1301:1317	certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one	1301:1437	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	2	29	theme	treatment	716:724	arg1	weeks					698:702	six weeks	694:702	six weeks of lysozyme treatment	694:724	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	1	30	theme	modified	212:219	arg1	scaffold					231:238	a uniquely modified composite scaffold	201:238	a uniquely modified composite scaffold	201:238	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	6	31	theme	in	1580:1581	arg1	studies					1588:1594	in vivo studies	1580:1594	in vivo studies for pre-clinical purposes	1580:1620	In the future, we plan to intensify our studies on osteogenic differentiation on our scaffolds on a detailed molecular level and to include in vivo studies for pre-clinical purposes.
28325318	1	32	theme	composite	221:229	arg1	scaffold					231:238	a uniquely modified composite scaffold	201:238	a uniquely modified composite scaffold	201:238	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	2	33	theme	CTS	560:562	arg1	scaffolds					577:585	CTS and CTS+nHAP scaffolds	560:585	scaffolds	577:585	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	5	34	theme	promising	1410:1418	arg1	scaffold					1386:1393	CTS+nHAP-NH2 scaffold	1373:1393	CTS+nHAP-NH2 scaffold	1373:1393	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	5	34	theme	promising	1410:1418	arg1	one					1435:1437	the most promising and applicable one	1401:1437	the most promising and applicable one	1401:1437	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	4	35	theme	initial	1114:1120	arg1	activity					1149:1156	initial alkaline phosphatase (ALP) activity	1114:1156	initial alkaline phosphatase (ALP) activity	1114:1156	Finally, during osteoinductivity assessment, CTS+nHAP-NH2 nearly tripled initial alkaline phosphatase (ALP) activity when whereas both CTS and CTS+nHAP scaffolds only doubled.
28325318	3	36	theme	least	912:916	arg1	level					933:937	the least and acceptable level	908:937	the least and acceptable level of cytotoxicity	908:953	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	6	37	from	studies	1480:1486	arg1	differentiation					1502:1516	osteogenic differentiation	1491:1516	osteogenic differentiation	1491:1516	In the future, we plan to intensify our studies on osteogenic differentiation on our scaffolds on a detailed molecular level and to include in vivo studies for pre-clinical purposes.
28325318	6	37	from	studies	1480:1486	arg1	scaffolds					1525:1533	our scaffolds	1521:1533	our scaffolds on a detailed molecular level	1521:1563	In the future, we plan to intensify our studies on osteogenic differentiation on our scaffolds on a detailed molecular level and to include in vivo studies for pre-clinical purposes.
28325318	0	38	theme	functional	42:51	arg1	nano-hydroxyapatite/chitosan					53:80	amine functional nano-hydroxyapatite/chitosan	36:80	amine functional nano-hydroxyapatite/chitosan	36:80	Preparation and characterization of amine functional nano-hydroxyapatite/chitosan bionanocomposite for bone tissue engineering applications.
28325318	1	39	theme	amine	320:324	arg1	NH2					333:335	NH2	333:335	NH2	333:335	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	1	39	theme	amine	320:324	arg1	group					326:330	amine group	320:330	amine group (NH2)	320:336	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	4	40	theme	osteoinductivity	1057:1072	arg1	assessment					1074:1083	osteoinductivity assessment	1057:1083	osteoinductivity assessment	1057:1083	Finally, during osteoinductivity assessment, CTS+nHAP-NH2 nearly tripled initial alkaline phosphatase (ALP) activity when whereas both CTS and CTS+nHAP scaffolds only doubled.
28325318	0	41	theme	amine	36:40	arg1	nano-hydroxyapatite/chitosan					53:80	amine functional nano-hydroxyapatite/chitosan	36:80	amine functional nano-hydroxyapatite/chitosan	36:80	Preparation and characterization of amine functional nano-hydroxyapatite/chitosan bionanocomposite for bone tissue engineering applications.
28325318	4	42	theme	alkaline	1122:1129	arg1	phosphatase					1131:1141	alkaline phosphatase	1122:1141	initial alkaline phosphatase (ALP) activity	1114:1156	Finally, during osteoinductivity assessment, CTS+nHAP-NH2 nearly tripled initial alkaline phosphatase (ALP) activity when whereas both CTS and CTS+nHAP scaffolds only doubled.
28325318	4	42	theme	alkaline	1122:1129	arg1	ALP					1144:1146	ALP	1144:1146	ALP	1144:1146	Finally, during osteoinductivity assessment, CTS+nHAP-NH2 nearly tripled initial alkaline phosphatase (ALP) activity when whereas both CTS and CTS+nHAP scaffolds only doubled.
28325318	3	43	theme	acceptable	922:931	arg1	level					933:937	the least and acceptable level	908:937	the least and acceptable level of cytotoxicity	908:953	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	1	44	theme	bone	434:437	arg1	engineering					446:456	bone tissue engineering	434:456	bone tissue engineering (BTE) purposes	434:471	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	1	44	theme	bone	434:437	arg1	BTE					459:461	BTE	459:461	BTE	459:461	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	2	45	theme	loss	683:686	arg1	loss					683:686	weight loss	676:686	weight loss	676:686	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	2	45	theme	loss	683:686	arg1	%					671:671	10%	669:671	10% of weight loss	669:686	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	0	46	theme	nano-hydroxyapatite/chitosan	53:80	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of amine functional nano-hydroxyapatite/chitosan bionanocomposite for bone tissue engineering applications.
28325318	0	46	theme	nano-hydroxyapatite/chitosan	53:80	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of amine functional nano-hydroxyapatite/chitosan bionanocomposite for bone tissue engineering applications.
28325318	1	47	theme	tissue	439:444	arg1	engineering					446:456	bone tissue engineering	434:456	bone tissue engineering (BTE) purposes	434:471	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	1	47	theme	tissue	439:444	arg1	BTE					459:461	BTE	459:461	BTE	459:461	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	2	48	theme	initial	613:619	arg1	weight					621:626	their initial weight	607:626	their initial weight	607:626	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	2	49	theme	weight	676:681	arg1	loss					683:686	weight loss	676:686	weight loss	676:686	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	2	50	with	biodegradable	541:553	arg1	scaffolds					577:585	CTS and CTS+nHAP scaffolds	560:585	scaffolds	577:585	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	2	51	theme	CTS+nHAP	568:575	arg1	scaffolds					577:585	CTS and CTS+nHAP scaffolds	560:585	scaffolds	577:585	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	1	52	theme	engineering	446:456	arg1	purposes					464:471	bone tissue engineering (BTE) purposes	434:471	bone tissue engineering (BTE) purposes	434:471	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	5	53	theme	CTS+nHAP-NH2	1373:1384	arg1	scaffold					1386:1393	CTS+nHAP-NH2 scaffold	1373:1393	CTS+nHAP-NH2 scaffold	1373:1393	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	5	53	theme	CTS+nHAP-NH2	1373:1384	arg1	one					1435:1437	the most promising and applicable one	1401:1437	the most promising and applicable one	1401:1437	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	3	54	theme	CTS+nHAP-NH2	818:829	arg1	scaffolds					831:839	CTS+nHAP-NH2 scaffolds	818:839	CTS+nHAP-NH2 scaffolds	818:839	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	6	55	theme	molecular	1549:1557	arg1	level					1559:1563	a detailed molecular level	1538:1563	a detailed molecular level	1538:1563	In the future, we plan to intensify our studies on osteogenic differentiation on our scaffolds on a detailed molecular level and to include in vivo studies for pre-clinical purposes.
28325318	2	56	theme	lysozyme	707:714	arg1	treatment					716:724	lysozyme treatment	707:724	lysozyme treatment	707:724	As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment.
28325318	1	57	theme	different	162:170	arg1	types					172:176	three different types	156:176	three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2)	156:336	In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes.
28325318	5	58	theme	synthesized	1249:1259	arg1	types					1270:1274	all synthesized scaffold types	1245:1274	all synthesized scaffold types under investigation	1245:1294	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	0	59	theme	bone	103:106	arg1	applications					127:138	bone tissue engineering applications	103:138	bone tissue engineering applications	103:138	Preparation and characterization of amine functional nano-hydroxyapatite/chitosan bionanocomposite for bone tissue engineering applications.
28325318	3	60	theme	lactate	958:964	arg1	LDH					981:983	LDH	981:983	LDH	981:983	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	3	60	theme	lactate	958:964	arg1	dehydrogenase					966:978	lactate dehydrogenase	958:978	lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs)	958:1038	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
28325318	5	61	theme	scaffold	1261:1268	arg1	types					1270:1274	all synthesized scaffold types	1245:1274	all synthesized scaffold types under investigation	1245:1294	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	6	62	dep	in	1580:1581	arg1	vivo					1583:1586	vivo	1583:1586	vivo	1583:1586	In the future, we plan to intensify our studies on osteogenic differentiation on our scaffolds on a detailed molecular level and to include in vivo studies for pre-clinical purposes.
28325318	5	63	theme	bone	1333:1336	arg1	tissue					1338:1343	bone tissue	1333:1343	bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one	1333:1437	These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS+nHAP-NH2 scaffold being the most promising and applicable one.
28325318	6	64	from	scaffolds	1525:1533	arg1	level					1559:1563	a detailed molecular level	1538:1563	a detailed molecular level	1538:1563	In the future, we plan to intensify our studies on osteogenic differentiation on our scaffolds on a detailed molecular level and to include in vivo studies for pre-clinical purposes.
28325318	3	65	theme	scaffolds	759:767	arg1	types					750:754	all three types	740:754	all three types of scaffolds	740:767	In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS+nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs).
29072617	5	0	theme	kinetic	638:644	arg1	model					646:650	a quasi-second-order kinetic model	617:650	a quasi-second-order kinetic model	617:650	The adsorption kinetics showed that ACH followed a quasi-second-order kinetic model.
29072617	2	1	theme	acrylic	351:357	arg1	acid					359:362	acrylic acid	351:362	acrylic acid	351:362	CCH was modified further by grafting acrylic acid to form a novel acrylated composite hydrogel (ACH).
29072617	5	2	theme	adsorption	572:581	arg1	kinetics					583:590	The adsorption kinetics	568:590	The adsorption kinetics	568:590	The adsorption kinetics showed that ACH followed a quasi-second-order kinetic model.
29072617	1	3	theme	triblock	152:159	arg1	F127					180:183	the triblock copolymer Pluronic F127	148:183	the triblock copolymer Pluronic F127	148:183	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	4	4	theme	Freundlich	550:559	arg1	model					561:565	the Freundlich model	546:565	the Freundlich model	546:565	Adsorption isotherms show that the adsorption process for methylene blue proximity fitted the Freundlich model.
29072617	7	5	theme	removal	821:827	arg1	rate					829:832	the removal rate	817:832	the removal rate of methylene blue at 75 mg/L	817:861	Repeated adsorption and desorption experiments were carried out three times, and the removal rate of methylene blue at 75 mg/L was still 70.1%.
29072617	7	5	theme	removal	821:827	arg1	%					877:877	70.1%	873:877	70.1%	873:877	Repeated adsorption and desorption experiments were carried out three times, and the removal rate of methylene blue at 75 mg/L was still 70.1%.
29072617	1	6	with	hydrogel	134:141	arg1	F127					180:183	the triblock copolymer Pluronic F127	148:183	the triblock copolymer Pluronic F127	148:183	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	7	7	from	mg/L	858:861	arg1	rate					829:832	the removal rate	817:832	the removal rate of methylene blue at 75 mg/L	817:861	Repeated adsorption and desorption experiments were carried out three times, and the removal rate of methylene blue at 75 mg/L was still 70.1%.
29072617	7	7	from	mg/L	858:861	arg1	%					877:877	70.1%	873:877	70.1%	873:877	Repeated adsorption and desorption experiments were carried out three times, and the removal rate of methylene blue at 75 mg/L was still 70.1%.
29072617	6	8	theme	temperature-	701:712	arg1	gel					731:733	a temperature- and pH-dependent gel	699:733	a temperature- and pH-dependent gel	699:733	Methylene blue desorption showed that ACH was a temperature- and pH-dependent gel.
29072617	6	8	theme	temperature-	701:712	arg1	ACH					691:693	ACH	691:693	ACH	691:693	Methylene blue desorption showed that ACH was a temperature- and pH-dependent gel.
29072617	1	9	theme	composite	260:268	arg1	hydrogel					298:305	a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel	258:305	a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH)	258:311	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	1	9	theme	composite	260:268	arg1	CCH					308:310	CCH	308:310	CCH	308:310	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	1	10	theme	copolymer	161:169	arg1	F127					180:183	the triblock copolymer Pluronic F127	148:183	the triblock copolymer Pluronic F127	148:183	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	4	11	theme	Adsorption	456:465	arg1	isotherms					467:475	Adsorption isotherms	456:475	Adsorption isotherms	456:475	Adsorption isotherms show that the adsorption process for methylene blue proximity fitted the Freundlich model.
29072617	7	12	theme	desorption	760:769	arg1	experiments					771:781	Repeated adsorption and desorption experiments	736:781	experiments	771:781	Repeated adsorption and desorption experiments were carried out three times, and the removal rate of methylene blue at 75 mg/L was still 70.1%.
29072617	7	13	theme	methylene	837:845	arg1	rate					829:832	the removal rate	817:832	the removal rate of methylene blue at 75 mg/L	817:861	Repeated adsorption and desorption experiments were carried out three times, and the removal rate of methylene blue at 75 mg/L was still 70.1%.
29072617	7	13	theme	methylene	837:845	arg1	%					877:877	70.1%	873:877	70.1%	873:877	Repeated adsorption and desorption experiments were carried out three times, and the removal rate of methylene blue at 75 mg/L was still 70.1%.
29072617	2	14	theme	novel	374:378	arg1	hydrogel					400:407	a novel acrylated composite hydrogel	372:407	a novel acrylated composite hydrogel (ACH)	372:413	CCH was modified further by grafting acrylic acid to form a novel acrylated composite hydrogel (ACH).
29072617	2	14	theme	novel	374:378	arg1	ACH					410:412	ACH	410:412	ACH	410:412	CCH was modified further by grafting acrylic acid to form a novel acrylated composite hydrogel (ACH).
29072617	0	15	theme	Composite	10:18	arg1	Preparation					29:39	Acrylated Composite Hydrogel Preparation	0:39	Acrylated Composite Hydrogel Preparation	0:39	Acrylated Composite Hydrogel Preparation and Adsorption Kinetics of Methylene Blue.
29072617	7	16	theme	blue	847:850	arg1	methylene					837:845	methylene blue	837:850	methylene blue	837:850	Repeated adsorption and desorption experiments were carried out three times, and the removal rate of methylene blue at 75 mg/L was still 70.1%.
29072617	0	17	theme	Acrylated	0:8	arg1	Preparation					29:39	Acrylated Composite Hydrogel Preparation	0:39	Acrylated Composite Hydrogel Preparation	0:39	Acrylated Composite Hydrogel Preparation and Adsorption Kinetics of Methylene Blue.
29072617	1	18	theme	CNC-β-CD/α-CD/Pluronic	270:291	arg1	hydrogel					298:305	a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel	258:305	a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH)	258:311	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	1	18	theme	CNC-β-CD/α-CD/Pluronic	270:291	arg1	CCH					308:310	CCH	308:310	CCH	308:310	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	5	19	theme	quasi-second-order	619:636	arg1	model					646:650	a quasi-second-order kinetic model	617:650	a quasi-second-order kinetic model	617:650	The adsorption kinetics showed that ACH followed a quasi-second-order kinetic model.
29072617	1	20	theme	cyclodextrin	93:104	arg1	self-assembly					113:125	cyclodextrin (α-CD) self-assembly	93:125	cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127	93:183	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	1	21	theme	F127	293:296	arg1	hydrogel					298:305	a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel	258:305	a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH)	258:311	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	1	21	theme	F127	293:296	arg1	CCH					308:310	CCH	308:310	CCH	308:310	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	3	22	theme	ACH	439:441	arg1	g/g					451:453	156 g/g	447:453	156 g/g	447:453	The swelling degree of ACH was 156 g/g.
29072617	3	22	theme	ACH	439:441	arg1	degree					429:434	The swelling degree	416:434	The swelling degree of ACH	416:441	The swelling degree of ACH was 156 g/g.
29072617	0	23	theme	Hydrogel	20:27	arg1	Preparation					29:39	Acrylated Composite Hydrogel Preparation	0:39	Acrylated Composite Hydrogel Preparation	0:39	Acrylated Composite Hydrogel Preparation and Adsorption Kinetics of Methylene Blue.
29072617	1	24	theme	α-CD	107:110	arg1	self-assembly					113:125	cyclodextrin (α-CD) self-assembly	93:125	cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127	93:183	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	0	25	theme	Adsorption	45:54	arg1	Kinetics					56:63	Adsorption Kinetics	45:63	Adsorption Kinetics	45:63	Acrylated Composite Hydrogel Preparation and Adsorption Kinetics of Methylene Blue.
29072617	6	26	theme	blue	663:666	arg1	desorption					668:677	Methylene blue desorption	653:677	Methylene blue desorption	653:677	Methylene blue desorption showed that ACH was a temperature- and pH-dependent gel.
29072617	2	27	theme	acrylated	380:388	arg1	hydrogel					400:407	a novel acrylated composite hydrogel	372:407	a novel acrylated composite hydrogel (ACH)	372:413	CCH was modified further by grafting acrylic acid to form a novel acrylated composite hydrogel (ACH).
29072617	2	27	theme	acrylated	380:388	arg1	ACH					410:412	ACH	410:412	ACH	410:412	CCH was modified further by grafting acrylic acid to form a novel acrylated composite hydrogel (ACH).
29072617	7	28	theme	Repeated	736:743	arg1	adsorption					745:754	Repeated adsorption and desorption experiments	736:781	adsorption	745:754	Repeated adsorption and desorption experiments were carried out three times, and the removal rate of methylene blue at 75 mg/L was still 70.1%.
29072617	2	29	theme	composite	390:398	arg1	hydrogel					400:407	a novel acrylated composite hydrogel	372:407	a novel acrylated composite hydrogel (ACH)	372:413	CCH was modified further by grafting acrylic acid to form a novel acrylated composite hydrogel (ACH).
29072617	2	29	theme	composite	390:398	arg1	ACH					410:412	ACH	410:412	ACH	410:412	CCH was modified further by grafting acrylic acid to form a novel acrylated composite hydrogel (ACH).
29072617	4	30	theme	adsorption	491:500	arg1	process					502:508	the adsorption process	487:508	the adsorption process for methylene blue proximity	487:537	Adsorption isotherms show that the adsorption process for methylene blue proximity fitted the Freundlich model.
29072617	4	31	theme	blue	524:527	arg1	proximity					529:537	methylene blue proximity	514:537	methylene blue proximity	514:537	Adsorption isotherms show that the adsorption process for methylene blue proximity fitted the Freundlich model.
29072617	3	32	theme	swelling	420:427	arg1	g/g					451:453	156 g/g	447:453	156 g/g	447:453	The swelling degree of ACH was 156 g/g.
29072617	3	32	theme	swelling	420:427	arg1	degree					429:434	The swelling degree	416:434	The swelling degree of ACH	416:441	The swelling degree of ACH was 156 g/g.
29072617	0	33	theme	Blue	78:81	arg1	Methylene					68:76	Methylene Blue	68:81	Methylene Blue	68:81	Acrylated Composite Hydrogel Preparation and Adsorption Kinetics of Methylene Blue.
29072617	1	34	theme	nanomicrocrystalline	186:205	arg1	cellulose					207:215	nanomicrocrystalline cellulose	186:215	nanomicrocrystalline cellulose	186:215	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	1	35	theme	Pluronic	171:178	arg1	F127					180:183	the triblock copolymer Pluronic F127	148:183	the triblock copolymer Pluronic F127	148:183	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	1	36	theme	gel	239:241	arg1	system					243:248	a gel system	237:248	a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH)	237:311	By using cyclodextrin (α-CD) self-assembly into a hydrogel with the triblock copolymer Pluronic F127, nanomicrocrystalline cellulose was introduced into a gel system to form a composite CNC-β-CD/α-CD/Pluronic F127 hydrogel (CCH).
29072617	0	37	theme	Methylene	68:76	arg1	Preparation					29:39	Acrylated Composite Hydrogel Preparation	0:39	Acrylated Composite Hydrogel Preparation	0:39	Acrylated Composite Hydrogel Preparation and Adsorption Kinetics of Methylene Blue.
29072617	0	37	theme	Methylene	68:76	arg1	Kinetics					56:63	Adsorption Kinetics	45:63	Adsorption Kinetics	45:63	Acrylated Composite Hydrogel Preparation and Adsorption Kinetics of Methylene Blue.
29072617	6	38	theme	pH-dependent	718:729	arg1	gel					731:733	a temperature- and pH-dependent gel	699:733	a temperature- and pH-dependent gel	699:733	Methylene blue desorption showed that ACH was a temperature- and pH-dependent gel.
29072617	6	38	theme	pH-dependent	718:729	arg1	ACH					691:693	ACH	691:693	ACH	691:693	Methylene blue desorption showed that ACH was a temperature- and pH-dependent gel.
28536024	0	0	theme	composite	65:73	arg1	chitosan					56:63	cerium immobilized cross-linked chitosan composite	24:73	cerium immobilized cross-linked chitosan composite in single system	24:90	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system and coexisted with Orange II in binary system.
28536024	1	1	dep	Cr	253:254	arg1	VI					256:257	VI	256:257	VI	256:257	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	1	2	link	cross-linked	172:183	arg1	composite					203:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	8	3	theme	same	1251:1254	arg1	temperature					1256:1266	the same temperature	1247:1266	the same temperature	1247:1266	|Besides, ||ΔGCr||>||ΔGCr-OII|| at the same temperature further suggested that Cr(VI) was adsorbed on the CTS-Ce composite faster in binary system than in single system.|
28536024	4	4	theme	OII	754:756	arg1	concentration					729:741	initial concentration	721:741	initial concentration of 100mg/L OII in binary system	721:773	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	4	5	from	concentration	729:741	arg1	system					768:773	binary system	761:773	binary system	761:773	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	8	6	from	system	1374:1379	arg1	composite					1325:1333	composite	1325:1333	composite	1325:1333	|Besides, ||ΔGCr||>||ΔGCr-OII|| at the same temperature further suggested that Cr(VI) was adsorbed on the CTS-Ce composite faster in binary system than in single system.|
28536024	3	7	theme	binary	519:524	arg1	system					526:531	single and binary system	508:531	single and binary system	508:531	The adsorption behaviors of Cr(VI) in single and binary system were systematically studied.
28536024	4	8	theme	initial	721:727	arg1	concentration					729:741	initial concentration	721:741	initial concentration of 100mg/L OII in binary system	721:773	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	0	9	theme	single	78:83	arg1	system					85:90	single system	78:90	single system	78:90	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system and coexisted with Orange II in binary system.
28536024	0	10	from	Adsorption	0:9	arg1	chitosan					56:63	cerium immobilized cross-linked chitosan composite	24:73	cerium immobilized cross-linked chitosan composite in single system	24:90	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system and coexisted with Orange II in binary system.
28536024	8	11	from	system	1352:1357	arg1	composite					1325:1333	composite	1325:1333	composite	1325:1333	|Besides, ||ΔGCr||>||ΔGCr-OII|| at the same temperature further suggested that Cr(VI) was adsorbed on the CTS-Ce composite faster in binary system than in single system.|
28536024	1	12	theme	co-precipitation	346:361	arg1	method					363:368	co-precipitation method	346:368	co-precipitation method	346:368	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	5	13	theme	adsorption	797:806	arg1	data					808:811	The adsorption data	793:811	The adsorption data for Cr	793:818	The adsorption data for Cr(VI) followed the Langmuir model in single system, while fitted Temkin model well in binary system.
28536024	6	14	theme	binary	938:943	arg1	system					945:950	both single and binary system	922:950	both single and binary system	922:950	In both single and binary system, the kinetics of adsorption exhibited pseudo-second order behavior and adsorption capacity increased with increasing temperature.
28536024	8	15	dep	adsorbed	1302:1309	arg1	VI					1294:1295	VI	1294:1295	VI	1294:1295	|Besides, ||ΔGCr||>||ΔGCr-OII|| at the same temperature further suggested that Cr(VI) was adsorbed on the CTS-Ce composite faster in binary system than in single system.|
28536024	8	16	theme	composite	1325:1333	arg1	CTS-Ce					1318:1323	the CTS-Ce	1314:1323	the CTS-Ce composite faster in binary system than in single system	1314:1379	|Besides, ||ΔGCr||>||ΔGCr-OII|| at the same temperature further suggested that Cr(VI) was adsorbed on the CTS-Ce composite faster in binary system than in single system.|
28536024	6	17	theme	adsorption	969:978	arg1	kinetics					957:964	the kinetics	953:964	the kinetics of adsorption	953:978	In both single and binary system, the kinetics of adsorption exhibited pseudo-second order behavior and adsorption capacity increased with increasing temperature.
28536024	5	18	theme	binary	904:909	arg1	system					911:916	binary system	904:916	binary system	904:916	The adsorption data for Cr(VI) followed the Langmuir model in single system, while fitted Temkin model well in binary system.
28536024	7	19	theme	endothermic	1196:1206	arg1	adsorption					1163:1172	the adsorption	1159:1172	the adsorption	1159:1172	Moreover, the data of thermodynamic parameters (ΔG°<0, ΔH°>0) indicated that the adsorption was a spontaneous and endothermic process.
28536024	7	19	theme	endothermic	1196:1206	arg1	process					1208:1214	a spontaneous and endothermic process	1178:1214	a spontaneous and endothermic process	1178:1214	Moreover, the data of thermodynamic parameters (ΔG°<0, ΔH°>0) indicated that the adsorption was a spontaneous and endothermic process.
28536024	4	20	from	capacity	585:592	arg1	202.8mg/g					615:623	202.8mg/g	615:623	202.8mg/g	615:623	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	4	20	from	capacity	585:592	arg1	CTS-Ce					607:612	CTS-Ce	607:612	CTS-Ce (202.8mg/g)	607:624	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	1	21	from	Cr	253:254	arg1	system					270:275	single system	263:275	single system	263:275	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	4	22	theme	Langmuir	644:651	arg1	equation					653:660	Langmuir equation	644:660	Langmuir equation in single metal system	644:683	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	8	23	from	temperature	1256:1266	arg1	|||ΔGCr||>||ΔGCr-OII|||					1226:1242	|||ΔGCr||>||ΔGCr-OII|||	1226:1242	|||ΔGCr||>||ΔGCr-OII|| at the same temperature|	1226:1266	|Besides, ||ΔGCr||>||ΔGCr-OII|| at the same temperature further suggested that Cr(VI) was adsorbed on the CTS-Ce composite faster in binary system than in single system.|
28536024	7	24	theme	thermodynamic	1104:1116	arg1	parameters					1118:1127	thermodynamic parameters	1104:1127	thermodynamic parameters (ΔG°<0, ΔH°>0)	1104:1142	Moreover, the data of thermodynamic parameters (ΔG°<0, ΔH°>0) indicated that the adsorption was a spontaneous and endothermic process.
28536024	5	25	dep	followed	824:831	arg1	VI					820:821	VI	820:821	VI	820:821	The adsorption data for Cr(VI) followed the Langmuir model in single system, while fitted Temkin model well in binary system.
28536024	3	26	dep	Cr	498:499	arg1	VI					501:502	VI	501:502	VI	501:502	The adsorption behaviors of Cr(VI) in single and binary system were systematically studied.
28536024	3	27	theme	adsorption	474:483	arg1	behaviors					485:493	The adsorption behaviors	470:493	The adsorption behaviors of Cr(VI) in single and binary system	470:531	The adsorption behaviors of Cr(VI) in single and binary system were systematically studied.
28536024	3	28	from	behaviors	485:493	arg1	system					526:531	single and binary system	508:531	single and binary system	508:531	The adsorption behaviors of Cr(VI) in single and binary system were systematically studied.
28536024	1	29	dep	composite	203:211	arg1	employed					214:221	employed	214:221	employed as an efficient adsorbent for Cr(VI) in single system	214:275	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	1	29	dep	composite	203:211	arg1	coexisted					281:289	coexisted	281:289	coexisted with Orange II (OII) in binary system	281:327	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	6	30	theme	order	1004:1008	arg1	behavior					1010:1017	pseudo-second order behavior	990:1017	pseudo-second order behavior	990:1017	In both single and binary system, the kinetics of adsorption exhibited pseudo-second order behavior and adsorption capacity increased with increasing temperature.
28536024	7	31	theme	spontaneous	1180:1190	arg1	adsorption					1163:1172	the adsorption	1159:1172	the adsorption	1159:1172	Moreover, the data of thermodynamic parameters (ΔG°<0, ΔH°>0) indicated that the adsorption was a spontaneous and endothermic process.
28536024	7	31	theme	spontaneous	1180:1190	arg1	process					1208:1214	a spontaneous and endothermic process	1178:1214	a spontaneous and endothermic process	1178:1214	Moreover, the data of thermodynamic parameters (ΔG°<0, ΔH°>0) indicated that the adsorption was a spontaneous and endothermic process.
28536024	1	32	theme	cerium	153:158	arg1	composite					203:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	0	33	theme	Cr	14:15	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system	0:90	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system and coexisted with Orange II in binary system.
28536024	4	34	dep	Cr	597:598	arg1	VI					600:601	VI	600:601	VI	600:601	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	1	35	theme	immobilized	160:170	arg1	composite					203:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	6	36	theme	single	927:932	arg1	system					945:950	both single and binary system	922:950	both single and binary system	922:950	In both single and binary system, the kinetics of adsorption exhibited pseudo-second order behavior and adsorption capacity increased with increasing temperature.
28536024	0	37	from	chitosan	56:63	arg1	system					85:90	single system	78:90	single system	78:90	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system and coexisted with Orange II in binary system.
28536024	3	38	theme	Cr	498:499	arg1	behaviors					485:493	The adsorption behaviors	470:493	The adsorption behaviors of Cr(VI) in single and binary system	470:531	The adsorption behaviors of Cr(VI) in single and binary system were systematically studied.
28536024	1	39	theme	cross-linked	172:183	arg1	composite					203:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	3	40	theme	single	508:513	arg1	system					526:531	single and binary system	508:531	single and binary system	508:531	The adsorption behaviors of Cr(VI) in single and binary system were systematically studied.
28536024	4	41	theme	metal	672:676	arg1	system					678:683	single metal system	665:683	single metal system	665:683	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	1	42	theme	chitosan	185:192	arg1	composite					203:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	0	43	theme	cerium	24:29	arg1	chitosan					56:63	cerium immobilized cross-linked chitosan composite	24:73	cerium immobilized cross-linked chitosan composite in single system	24:90	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system and coexisted with Orange II in binary system.
28536024	8	44	theme	single	1367:1372	arg1	system					1374:1379	single system	1367:1379	single system	1367:1379	|Besides, ||ΔGCr||>||ΔGCr-OII|| at the same temperature further suggested that Cr(VI) was adsorbed on the CTS-Ce composite faster in binary system than in single system.|
28536024	0	45	theme	binary	124:129	arg1	system					131:136	binary system	124:136	binary system	124:136	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system and coexisted with Orange II in binary system.
28536024	1	46	theme	binary	315:320	arg1	system					322:327	binary system	315:327	binary system	315:327	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	8	47	from	composite	1325:1333	arg1	system					1374:1379	single system	1367:1379	single system	1367:1379	|Besides, ||ΔGCr||>||ΔGCr-OII|| at the same temperature further suggested that Cr(VI) was adsorbed on the CTS-Ce composite faster in binary system than in single system.|
28536024	8	47	from	composite	1325:1333	arg1	system					1352:1357	binary system	1345:1357	binary system	1345:1357	|Besides, ||ΔGCr||>||ΔGCr-OII|| at the same temperature further suggested that Cr(VI) was adsorbed on the CTS-Ce composite faster in binary system than in single system.|
28536024	4	48	theme	100mg/L	746:752	arg1	OII					754:756	100mg/L OII	746:756	100mg/L OII	746:756	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	5	49	theme	single	855:860	arg1	system					862:867	single system	855:867	single system	855:867	The adsorption data for Cr(VI) followed the Langmuir model in single system, while fitted Temkin model well in binary system.
28536024	6	50	theme	adsorption	1023:1032	arg1	capacity					1034:1041	adsorption capacity	1023:1041	adsorption capacity	1023:1041	In both single and binary system, the kinetics of adsorption exhibited pseudo-second order behavior and adsorption capacity increased with increasing temperature.
28536024	0	51	link	cross-linked	43:54	arg1	chitosan					56:63	cerium immobilized cross-linked chitosan composite	24:73	cerium immobilized cross-linked chitosan composite in single system	24:90	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system and coexisted with Orange II in binary system.
28536024	4	52	theme	Cr	597:598	arg1	capacity					585:592	The maximum adsorption capacity	562:592	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g)	562:624	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	7	53	dep	parameters	1118:1127	arg1	ΔH°>0					1137:1141	ΔH°>0	1137:1141	ΔH°>0	1137:1141	Moreover, the data of thermodynamic parameters (ΔG°<0, ΔH°>0) indicated that the adsorption was a spontaneous and endothermic process.
28536024	7	53	dep	parameters	1118:1127	arg1	ΔG°<0					1130:1134	ΔG°<0	1130:1134	ΔG°<0	1130:1134	Moreover, the data of thermodynamic parameters (ΔG°<0, ΔH°>0) indicated that the adsorption was a spontaneous and endothermic process.
28536024	4	54	theme	binary	761:766	arg1	system					768:773	binary system	761:773	binary system	761:773	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	5	55	theme	Langmuir	837:844	arg1	model					846:850	the Langmuir model	833:850	the Langmuir model	833:850	The adsorption data for Cr(VI) followed the Langmuir model in single system, while fitted Temkin model well in binary system.
28536024	0	56	dep	Cr	14:15	arg1	VI					17:18	VI	17:18	VI	17:18	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system and coexisted with Orange II in binary system.
28536024	5	57	theme	Temkin	883:888	arg1	model					890:894	Temkin model	883:894	Temkin model	883:894	The adsorption data for Cr(VI) followed the Langmuir model in single system, while fitted Temkin model well in binary system.
28536024	1	58	theme	CTS-Ce	195:200	arg1	composite					203:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	cerium immobilized cross-linked chitosan (CTS-Ce) composite	153:211	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	0	59	theme	cross-linked	43:54	arg1	chitosan					56:63	cerium immobilized cross-linked chitosan composite	24:73	cerium immobilized cross-linked chitosan composite in single system	24:90	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system and coexisted with Orange II in binary system.
28536024	7	60	theme	parameters	1118:1127	arg1	data					1096:1099	the data	1092:1099	the data of thermodynamic parameters (ΔG°<0, ΔH°>0)	1092:1142	Moreover, the data of thermodynamic parameters (ΔG°<0, ΔH°>0) indicated that the adsorption was a spontaneous and endothermic process.
28536024	4	61	theme	single	665:670	arg1	system					678:683	single metal system	665:683	single metal system	665:683	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	8	62	theme	binary	1345:1350	arg1	system					1352:1357	binary system	1345:1357	binary system	1345:1357	|Besides, ||ΔGCr||>||ΔGCr-OII|| at the same temperature further suggested that Cr(VI) was adsorbed on the CTS-Ce composite faster in binary system than in single system.|
28536024	4	63	theme	adsorption	574:583	arg1	capacity					585:592	The maximum adsorption capacity	562:592	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g)	562:624	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	8	64	dep	composite	1325:1333	arg1	faster					1335:1340	faster	1335:1340	faster	1335:1340	|Besides, ||ΔGCr||>||ΔGCr-OII|| at the same temperature further suggested that Cr(VI) was adsorbed on the CTS-Ce composite faster in binary system than in single system.|
28536024	0	65	theme	immobilized	31:41	arg1	chitosan					56:63	cerium immobilized cross-linked chitosan composite	24:73	cerium immobilized cross-linked chitosan composite in single system	24:90	Adsorption of Cr(VI) on cerium immobilized cross-linked chitosan composite in single system and coexisted with Orange II in binary system.
28536024	1	66	theme	single	263:268	arg1	system					270:275	single system	263:275	single system	263:275	In this work, cerium immobilized cross-linked chitosan (CTS-Ce) composite, employed as an efficient adsorbent for Cr(VI) in single system and coexisted with Orange II (OII) in binary system, was prepared by co-precipitation method.
28536024	6	67	theme	pseudo-second	990:1002	arg1	behavior					1010:1017	pseudo-second order behavior	990:1017	pseudo-second order behavior	990:1017	In both single and binary system, the kinetics of adsorption exhibited pseudo-second order behavior and adsorption capacity increased with increasing temperature.
28536024	4	68	from	equation	653:660	arg1	system					678:683	single metal system	665:683	single metal system	665:683	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	4	69	theme	maximum	566:572	arg1	capacity					585:592	The maximum adsorption capacity	562:592	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g)	562:624	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
28536024	4	70	with	112.9mg/g	706:714	arg1	concentration					729:741	initial concentration	721:741	initial concentration of 100mg/L OII in binary system	721:773	The maximum adsorption capacity of Cr(VI) on CTS-Ce (202.8mg/g) was calculated by Langmuir equation in single metal system, but it decreased to 112.9mg/g with initial concentration of 100mg/L OII in binary system at pH 2 and 293K.
25596416	6	0	theme	silicon	1011:1017	arg1	carbides					1019:1026	biomorphic silicon carbides	1000:1026	biomorphic silicon carbides	1000:1026	Particles derived from mechanical wear of biomorphic silicon carbides do not show high toxicity, being similar to the zirconia particles.
25596416	4	1	theme	pine	761:764	arg1	composites					773:782	pine bioSiC composites	761:782	pine bioSiC composites	761:782	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	6	2	theme	biomorphic	1000:1009	arg1	carbides					1019:1026	biomorphic silicon carbides	1000:1026	biomorphic silicon carbides	1000:1026	Particles derived from mechanical wear of biomorphic silicon carbides do not show high toxicity, being similar to the zirconia particles.
25596416	6	3	theme	zirconia	1076:1083	arg1	particles					1085:1093	the zirconia particles	1072:1093	the zirconia particles	1072:1093	Particles derived from mechanical wear of biomorphic silicon carbides do not show high toxicity, being similar to the zirconia particles.
25596416	5	4	theme	released	789:796	arg1	able					814:817	able	814:817	able	814:817	The released indomethacin is able to modulate the degradation of chondrocytes extracellular matrix and promote the formation of new collagen by osteoarthritic chondrocytes.
25596416	5	4	theme	released	789:796	arg1	indomethacin					798:809	The released indomethacin	785:809	The released indomethacin	785:809	The released indomethacin is able to modulate the degradation of chondrocytes extracellular matrix and promote the formation of new collagen by osteoarthritic chondrocytes.
25596416	0	5	from	composites	75:84	arg1	release					11:17	Controlled release	0:17	Controlled release of indomethacin from alginate-poloxamer-silicon carbide composites	0:84	Controlled release of indomethacin from alginate-poloxamer-silicon carbide composites decrease in-vitro inflammation.
25596416	4	6	contain	have	578:581	arg1	composites					567:576	The loaded oak and sapelli bioSiCs composites	532:576	composites	567:576	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	4	6	contain	have	578:581	arg2	profiles					600:607	adequate release profiles	583:607	adequate release profiles	583:607	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	4	6	contain	have	578:581	arg1	oak					543:545	The loaded oak and sapelli bioSiCs composites	532:576	oak	543:545	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	5	7	theme	new	913:915	arg1	collagen					917:924	new collagen	913:924	new collagen	913:924	The released indomethacin is able to modulate the degradation of chondrocytes extracellular matrix and promote the formation of new collagen by osteoarthritic chondrocytes.
25596416	4	8	theme	cytokines	672:680	arg1	secretion					642:650	the secretion	638:650	the secretion of pro-inflammatory cytokines	638:680	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	4	9	theme	stronger	721:728	arg1	effects					748:754	stronger anti-inflammatory effects	721:754	stronger anti-inflammatory effects than pine bioSiC composites	721:782	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	5	10	theme	osteoarthritic	929:942	arg1	chondrocytes					944:955	osteoarthritic chondrocytes	929:955	osteoarthritic chondrocytes	929:955	The released indomethacin is able to modulate the degradation of chondrocytes extracellular matrix and promote the formation of new collagen by osteoarthritic chondrocytes.
25596416	6	11	attach	derived	968:974	arg2	Particles					958:966	Particles	958:966	Particles derived from mechanical wear of biomorphic silicon carbides	958:1026	Particles derived from mechanical wear of biomorphic silicon carbides do not show high toxicity, being similar to the zirconia particles.
25596416	6	11	attach	derived	968:974	arg1	wear					992:995	wear	992:995	wear	992:995	Particles derived from mechanical wear of biomorphic silicon carbides do not show high toxicity, being similar to the zirconia particles.
25596416	4	12	theme	release	592:598	arg1	profiles					600:607	adequate release profiles	583:607	adequate release profiles	583:607	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	3	13	theme	release	457:463	arg1	dependent					476:484	dependent	476:484	dependent	476:484	The indomethacin release profile is dependent on the microstructure of the bioSiC selected.
25596416	3	13	theme	release	457:463	arg1	profile					465:471	The indomethacin release profile	440:471	The indomethacin release profile	440:471	The indomethacin release profile is dependent on the microstructure of the bioSiC selected.
25596416	0	14	theme	Controlled	0:9	arg1	release					11:17	Controlled release	0:17	Controlled release of indomethacin from alginate-poloxamer-silicon carbide composites	0:84	Controlled release of indomethacin from alginate-poloxamer-silicon carbide composites decrease in-vitro inflammation.
25596416	1	15	theme	bone	290:293	arg1	therapy					307:313	bone pathologies therapy	290:313	bone pathologies therapy	290:313	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	5	16	dep	chondrocytes	850:861	arg1	matrix					877:882	extracellular matrix	863:882	chondrocytes extracellular matrix	850:882	The released indomethacin is able to modulate the degradation of chondrocytes extracellular matrix and promote the formation of new collagen by osteoarthritic chondrocytes.
25596416	0	17	theme	indomethacin	22:33	arg1	release					11:17	Controlled release	0:17	Controlled release of indomethacin from alginate-poloxamer-silicon carbide composites	0:84	Controlled release of indomethacin from alginate-poloxamer-silicon carbide composites decrease in-vitro inflammation.
25596416	1	18	theme	pathologies	295:305	arg1	therapy					307:313	bone pathologies therapy	290:313	bone pathologies therapy	290:313	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	1	19	from	application	275:285	arg1	therapy					307:313	bone pathologies therapy	290:313	bone pathologies therapy	290:313	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	1	20	theme	biomorphic	132:141	arg1	bioSiCs					161:167	bioSiCs	161:167	bioSiCs	161:167	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	1	20	theme	biomorphic	132:141	arg1	carbides					151:158	biomorphic silicon carbides	132:158	biomorphic silicon carbides (bioSiCs)	132:168	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	1	21	theme	silicon	143:149	arg1	bioSiCs					161:167	bioSiCs	161:167	bioSiCs	161:167	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	1	21	theme	silicon	143:149	arg1	carbides					151:158	biomorphic silicon carbides	132:158	biomorphic silicon carbides (bioSiCs)	132:168	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	1	22	theme	able	226:229	arg1	materials					216:224	materials	216:224	materials able to load and release poor soluble drugs	216:268	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	0	23	theme	alginate-poloxamer-silicon	40:65	arg1	composites					75:84	alginate-poloxamer-silicon carbide composites	40:84	alginate-poloxamer-silicon carbide composites	40:84	Controlled release of indomethacin from alginate-poloxamer-silicon carbide composites decrease in-vitro inflammation.
25596416	5	24	theme	collagen	917:924	arg1	formation					900:908	the formation	896:908	the formation of new collagen by osteoarthritic chondrocytes	896:955	The released indomethacin is able to modulate the degradation of chondrocytes extracellular matrix and promote the formation of new collagen by osteoarthritic chondrocytes.
25596416	3	25	theme	indomethacin	444:455	arg1	dependent					476:484	dependent	476:484	dependent	476:484	The indomethacin release profile is dependent on the microstructure of the bioSiC selected.
25596416	3	25	theme	indomethacin	444:455	arg1	profile					465:471	The indomethacin release profile	440:471	The indomethacin release profile	440:471	The indomethacin release profile is dependent on the microstructure of the bioSiC selected.
25596416	4	26	theme	bioSiCs	559:565	arg1	composites					567:576	The loaded oak and sapelli bioSiCs composites	532:576	composites	567:576	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	5	27	theme	chondrocytes	850:861	arg1	degradation					835:845	the degradation	831:845	the degradation of chondrocytes extracellular matrix	831:882	The released indomethacin is able to modulate the degradation of chondrocytes extracellular matrix and promote the formation of new collagen by osteoarthritic chondrocytes.
25596416	4	28	theme	pro-inflammatory	655:670	arg1	cytokines					672:680	pro-inflammatory cytokines	655:680	pro-inflammatory cytokines	655:680	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	5	29	theme	extracellular	863:875	arg1	matrix					877:882	extracellular matrix	863:882	chondrocytes extracellular matrix	850:882	The released indomethacin is able to modulate the degradation of chondrocytes extracellular matrix and promote the formation of new collagen by osteoarthritic chondrocytes.
25596416	4	30	theme	sapelli	551:557	arg1	composites					567:576	The loaded oak and sapelli bioSiCs composites	532:576	composites	567:576	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	4	31	theme	adequate	583:590	arg1	profiles					600:607	adequate release profiles	583:607	adequate release profiles	583:607	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	1	32	theme	carbides	151:158	arg1	hydrogels					174:182	hydrogels	174:182	hydrogels	174:182	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	1	32	theme	carbides	151:158	arg1	carbides					151:158	biomorphic silicon carbides	132:158	biomorphic silicon carbides (bioSiCs)	132:168	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	1	32	theme	carbides	151:158	arg1	Composites					118:127	Composites	118:127	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels	118:182	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	0	33	theme	carbide	67:73	arg1	composites					75:84	alginate-poloxamer-silicon carbide composites	40:84	alginate-poloxamer-silicon carbide composites	40:84	Controlled release of indomethacin from alginate-poloxamer-silicon carbide composites decrease in-vitro inflammation.
25596416	6	34	theme	carbides	1019:1026	arg1	wear					992:995	wear	992:995	wear	992:995	Particles derived from mechanical wear of biomorphic silicon carbides do not show high toxicity, being similar to the zirconia particles.
25596416	4	35	theme	bioSiC	766:771	arg1	composites					773:782	pine bioSiC composites	761:782	pine bioSiC composites	761:782	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	6	36	theme	high	1040:1043	arg1	toxicity					1045:1052	high toxicity	1040:1052	high toxicity	1040:1052	Particles derived from mechanical wear of biomorphic silicon carbides do not show high toxicity, being similar to the zirconia particles.
25596416	4	37	theme	anti-inflammatory	730:746	arg1	effects					748:754	stronger anti-inflammatory effects	721:754	stronger anti-inflammatory effects than pine bioSiC composites	721:782	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	1	38	dep	load	234:237	arg1	drugs					264:268	poor soluble drugs	251:268	poor soluble drugs	251:268	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	3	39	theme	bioSiC	515:520	arg1	microstructure					493:506	the microstructure	489:506	the microstructure of the bioSiC selected	489:529	The indomethacin release profile is dependent on the microstructure of the bioSiC selected.
25596416	0	40	theme	in-vitro	95:102	arg1	inflammation					104:115	in-vitro inflammation	95:115	in-vitro inflammation	95:115	Controlled release of indomethacin from alginate-poloxamer-silicon carbide composites decrease in-vitro inflammation.
25596416	1	41	theme	poor	251:254	arg1	drugs					264:268	poor soluble drugs	251:268	poor soluble drugs	251:268	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	4	42	theme	loaded	536:541	arg1	oak					543:545	The loaded oak and sapelli bioSiCs composites	532:576	oak	543:545	The loaded oak and sapelli bioSiCs composites have adequate release profiles to promote the decreasing of the secretion of pro-inflammatory cytokines in LPS stimulated macrophages, showing stronger anti-inflammatory effects than pine bioSiC composites.
25596416	1	43	theme	hydrogels	174:182	arg1	hydrogels					174:182	hydrogels	174:182	hydrogels	174:182	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	1	43	theme	hydrogels	174:182	arg1	carbides					151:158	biomorphic silicon carbides	132:158	biomorphic silicon carbides (bioSiCs)	132:168	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	1	43	theme	hydrogels	174:182	arg1	Composites					118:127	Composites	118:127	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels	118:182	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25596416	1	44	theme	soluble	256:262	arg1	drugs					264:268	poor soluble drugs	251:268	poor soluble drugs	251:268	Composites of biomorphic silicon carbides (bioSiCs) and hydrogels are proposed in order to obtain materials able to load and release poor soluble drugs with application in bone pathologies therapy.
25754046	7	0	theme	laborious	1258:1266	arg1	methods					1283:1289	laborious and unreliable methods	1258:1289	laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA	1258:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	7	1	theme	reliable	1166:1173	arg1	determination					1175:1187	fast and reliable determination	1157:1187	fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA	1157:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	2	2	theme	catalytic	392:400	arg1	kcat/KM					414:420	kcat/KM	414:420	kcat/KM	414:420	The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme.
25754046	2	2	theme	catalytic	392:400	arg1	efficiency					402:411	maximal catalytic efficiency	384:411	maximal catalytic efficiency (kcat/KM)	384:421	The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme.
25754046	6	3	theme	new	929:931	arg1	approach					943:950	a new versatile approach	927:950	a new versatile approach based on IR spectroscopy	927:975	It is worth mentioning that a new versatile approach based on IR spectroscopy has been developed to determine PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates.
25754046	1	4	theme	Erwinia	290:296	arg1	carotovora					298:307	Erwinia carotovora	290:307	Erwinia carotovora (EwA)	290:313	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	1	4	theme	Erwinia	290:296	arg1	EwA					310:312	EwA	310:312	EwA	310:312	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	2	5	theme	native	511:516	arg1	enzyme					518:523	the native enzyme	507:523	the native enzyme	507:523	The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme.
25754046	7	6	with	titration	1325:1333	arg1	OPA					1349:1351	OPA	1349:1351	OPA	1349:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	7	6	with	titration	1325:1333	arg1	TNBS					1340:1343	TNBS	1340:1343	TNBS	1340:1343	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	2	7	theme	maximal	384:390	arg1	kcat/KM					414:420	kcat/KM	414:420	kcat/KM	414:420	The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme.
25754046	2	7	theme	maximal	384:390	arg1	efficiency					402:411	maximal catalytic efficiency	384:411	maximal catalytic efficiency (kcat/KM)	384:421	The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme.
25754046	7	8	from	spectrum	1234:1241	arg1	contrast					1246:1253	contrast	1246:1253	contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA	1246:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	7	8	from	spectrum	1234:1241	arg1	determination					1175:1187	fast and reliable determination	1157:1187	fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA	1157:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	6	9	theme	versatile	933:941	arg1	approach					943:950	a new versatile approach	927:950	a new versatile approach based on IR spectroscopy	927:975	It is worth mentioning that a new versatile approach based on IR spectroscopy has been developed to determine PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates.
25754046	7	10	theme	fast	1157:1160	arg1	determination					1175:1187	fast and reliable determination	1157:1187	fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA	1157:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	0	11	theme	Erwinia	116:122	arg1	carotovora					124:133	Erwinia carotovora	116:133	Erwinia carotovora	116:133	PEG-chitosan and glycol-chitosan for improvement of biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora.
25754046	0	12	from	properties	70:79	arg1	carotovora					124:133	Erwinia carotovora	116:133	Erwinia carotovora	116:133	PEG-chitosan and glycol-chitosan for improvement of biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora.
25754046	6	13	theme	copolymer	1022:1030	arg1	composition					1032:1042	PEG-chitosan copolymer composition	1009:1042	PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates	1009:1096	It is worth mentioning that a new versatile approach based on IR spectroscopy has been developed to determine PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates.
25754046	2	14	dep	structure	320:328	arg1	The					316:318	The	316:318	The	316:318	The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme.
25754046	2	15	theme	conjugates	354:363	arg1	composition					334:344	composition	334:344	composition	334:344	The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme.
25754046	2	15	theme	conjugates	354:363	arg1	structure					320:328	structure	320:328	structure	320:328	The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme.
25754046	2	16	theme	physiological	429:441	arg1	conditions					443:452	physiological conditions	429:452	physiological conditions	429:452	The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme.
25754046	0	17	theme	biopharmaceutical	52:68	arg1	properties					70:79	biopharmaceutical properties	52:79	biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora	52:133	PEG-chitosan and glycol-chitosan for improvement of biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora.
25754046	7	18	theme	IR	1231:1232	arg1	spectrum					1234:1241	the single IR spectrum	1220:1241	the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA	1220:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	7	19	theme	single	1224:1229	arg1	spectrum					1234:1241	the single IR spectrum	1220:1241	the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA	1220:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	0	20	theme	L-asparaginase	96:109	arg1	properties					70:79	biopharmaceutical properties	52:79	biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora	52:133	PEG-chitosan and glycol-chitosan for improvement of biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora.
25754046	7	21	theme	reagent-free	1132:1143	arg1	method					1121:1126	The proposed analytic method	1099:1126	The proposed analytic method	1099:1126	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	7	21	theme	reagent-free	1132:1143	arg1	"					1144:1144	"reagent-free"	1131:1144	"reagent-free"	1131:1144	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	7	22	theme	unreliable	1272:1281	arg1	methods					1283:1289	laborious and unreliable methods	1258:1289	laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA	1258:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	2	23	theme	such	349:352	arg1	conjugates					354:363	such conjugates	349:363	such conjugates	349:363	The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme.
25754046	7	24	theme	group	1319:1323	arg1	titration					1325:1333	polymer free amino group titration	1300:1333	polymer free amino group titration with TNBS and OPA	1300:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	4	25	theme	EwA	691:693	arg1	conjugates					695:704	EwA conjugates	691:704	EwA conjugates	691:704	The thermostability of EwA conjugates was also considerably improved.
25754046	1	26	with	Conjugation	136:146	arg1	glycol-chitosan					199:213	glycol-chitosan	199:213	glycol-chitosan	199:213	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	1	26	with	Conjugation	136:146	arg1	PEG-chitosan					182:193	PEG-chitosan	182:193	PEG-chitosan	182:193	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	1	26	with	Conjugation	136:146	arg1	copolymers					170:179	the new branched copolymers	153:179	the new branched copolymers	153:179	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	6	27	theme	PEG-chitosan	1009:1020	arg1	composition					1032:1042	PEG-chitosan copolymer composition	1009:1042	PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates	1009:1096	It is worth mentioning that a new versatile approach based on IR spectroscopy has been developed to determine PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates.
25754046	3	28	theme	pH	575:576	arg1	profile					587:593	pH activity profile	575:593	pH activity profile	575:593	This effect is attributed mainly to the shift of pH activity profile towards lower pH values due to the polycationic nature of the copolymer.
25754046	3	29	theme	copolymer	657:665	arg1	nature					643:648	the polycationic nature	626:648	the polycationic nature of the copolymer	626:665	This effect is attributed mainly to the shift of pH activity profile towards lower pH values due to the polycationic nature of the copolymer.
25754046	3	30	theme	activity	578:585	arg1	profile					587:593	pH activity profile	575:593	pH activity profile	575:593	This effect is attributed mainly to the shift of pH activity profile towards lower pH values due to the polycationic nature of the copolymer.
25754046	7	31	theme	interest	1206:1213	arg1	parameters					1192:1201	parameters	1192:1201	parameters of interest	1192:1213	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	7	32	theme	analytic	1112:1119	arg1	method					1121:1126	The proposed analytic method	1099:1126	The proposed analytic method	1099:1126	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	7	32	theme	analytic	1112:1119	arg1	"					1144:1144	"reagent-free"	1131:1144	"reagent-free"	1131:1144	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	5	33	theme	relevant	882:889	arg1	enzyme					891:896	this medically relevant enzyme	867:896	this medically relevant enzyme	867:896	Chito-PEGylation, similarly to PEGylation, can be expected to improve pharmacokinetic properties and to reduce immunogenicity of this medically relevant enzyme.
25754046	5	34	theme	pharmacokinetic	808:822	arg1	properties					824:833	pharmacokinetic properties	808:833	pharmacokinetic properties	808:833	Chito-PEGylation, similarly to PEGylation, can be expected to improve pharmacokinetic properties and to reduce immunogenicity of this medically relevant enzyme.
25754046	1	35	from	carotovora	298:307	arg1	L-asparaginase					270:283	L-asparaginase	270:283	L-asparaginase from Erwinia carotovora (EwA)	270:313	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	1	35	from	carotovora	298:307	arg1	properties					256:265	the therapeutic properties	240:265	the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA)	240:313	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	7	36	theme	parameters	1192:1201	arg1	determination					1175:1187	fast and reliable determination	1157:1187	fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA	1157:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	1	37	theme	therapeutic	244:254	arg1	properties					256:265	the therapeutic properties	240:265	the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA)	240:313	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	0	38	theme	properties	70:79	arg1	glycol-chitosan					17:31	glycol-chitosan	17:31	glycol-chitosan	17:31	PEG-chitosan and glycol-chitosan for improvement of biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora.
25754046	0	38	theme	properties	70:79	arg1	PEG-chitosan					0:11	PEG-chitosan	0:11	PEG-chitosan	0:11	PEG-chitosan and glycol-chitosan for improvement of biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora.
25754046	6	39	theme	conjugates	1087:1096	arg1	composition					1055:1065	composition	1055:1065	PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates	1009:1096	It is worth mentioning that a new versatile approach based on IR spectroscopy has been developed to determine PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates.
25754046	6	39	theme	conjugates	1087:1096	arg1	composition					1032:1042	PEG-chitosan copolymer composition	1009:1042	PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates	1009:1096	It is worth mentioning that a new versatile approach based on IR spectroscopy has been developed to determine PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates.
25754046	3	40	theme	lower	603:607	arg1	values					612:617	lower pH values	603:617	lower pH values	603:617	This effect is attributed mainly to the shift of pH activity profile towards lower pH values due to the polycationic nature of the copolymer.
25754046	2	41	theme	3-6	491:493	arg1	factor					481:486	a factor	479:486	a factor of 3-6	479:493	The structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/KM) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme.
25754046	7	42	theme	proposed	1103:1110	arg1	method					1121:1126	The proposed analytic method	1099:1126	The proposed analytic method	1099:1126	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	7	42	theme	proposed	1103:1110	arg1	"					1144:1144	"reagent-free"	1131:1144	"reagent-free"	1131:1144	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	3	43	theme	pH	609:610	arg1	values					612:617	lower pH values	603:617	lower pH values	603:617	This effect is attributed mainly to the shift of pH activity profile towards lower pH values due to the polycationic nature of the copolymer.
25754046	3	44	theme	profile	587:593	arg1	shift					566:570	the shift	562:570	the shift of pH activity profile towards lower pH values due to the polycationic nature of the copolymer	562:665	This effect is attributed mainly to the shift of pH activity profile towards lower pH values due to the polycationic nature of the copolymer.
25754046	1	45	from	properties	256:265	arg1	carotovora					298:307	Erwinia carotovora	290:307	Erwinia carotovora (EwA)	290:313	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	1	45	from	properties	256:265	arg1	EwA					310:312	EwA	310:312	EwA	310:312	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	3	46	theme	polycationic	630:641	arg1	nature					643:648	the polycationic nature	626:648	the polycationic nature of the copolymer	626:665	This effect is attributed mainly to the shift of pH activity profile towards lower pH values due to the polycationic nature of the copolymer.
25754046	7	47	theme	free	1308:1311	arg1	titration					1325:1333	polymer free amino group titration	1300:1333	polymer free amino group titration with TNBS and OPA	1300:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	1	48	theme	new	157:159	arg1	glycol-chitosan					199:213	glycol-chitosan	199:213	glycol-chitosan	199:213	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	1	48	theme	new	157:159	arg1	PEG-chitosan					182:193	PEG-chitosan	182:193	PEG-chitosan	182:193	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	1	48	theme	new	157:159	arg1	copolymers					170:179	the new branched copolymers	153:179	the new branched copolymers	153:179	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	6	49	theme	copolymer-enzyme	1070:1085	arg1	conjugates					1087:1096	copolymer-enzyme conjugates	1070:1096	copolymer-enzyme conjugates	1070:1096	It is worth mentioning that a new versatile approach based on IR spectroscopy has been developed to determine PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates.
25754046	0	50	from	carotovora	124:133	arg1	L-asparaginase					96:109	recombinant L-asparaginase	84:109	recombinant L-asparaginase from Erwinia carotovora	84:133	PEG-chitosan and glycol-chitosan for improvement of biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora.
25754046	0	50	from	carotovora	124:133	arg1	properties					70:79	biopharmaceutical properties	52:79	biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora	52:133	PEG-chitosan and glycol-chitosan for improvement of biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora.
25754046	1	51	theme	branched	161:168	arg1	glycol-chitosan					199:213	glycol-chitosan	199:213	glycol-chitosan	199:213	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	1	51	theme	branched	161:168	arg1	PEG-chitosan					182:193	PEG-chitosan	182:193	PEG-chitosan	182:193	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	1	51	theme	branched	161:168	arg1	copolymers					170:179	the new branched copolymers	153:179	the new branched copolymers	153:179	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	7	52	theme	amino	1313:1317	arg1	titration					1325:1333	polymer free amino group titration	1300:1333	polymer free amino group titration with TNBS and OPA	1300:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	0	53	theme	recombinant	84:94	arg1	L-asparaginase					96:109	recombinant L-asparaginase	84:109	recombinant L-asparaginase from Erwinia carotovora	84:133	PEG-chitosan and glycol-chitosan for improvement of biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora.
25754046	5	54	theme	enzyme	891:896	arg1	immunogenicity					849:862	immunogenicity	849:862	immunogenicity of this medically relevant enzyme	849:896	Chito-PEGylation, similarly to PEGylation, can be expected to improve pharmacokinetic properties and to reduce immunogenicity of this medically relevant enzyme.
25754046	7	55	theme	polymer	1300:1306	arg1	titration					1325:1333	polymer free amino group titration	1300:1333	polymer free amino group titration with TNBS and OPA	1300:1351	The proposed analytic method is "reagent-free" and allows fast and reliable determination of parameters of interest from the single IR spectrum in contrast to laborious and unreliable methods based on polymer free amino group titration with TNBS and OPA.
25754046	1	56	theme	L-asparaginase	270:283	arg1	properties					256:265	the therapeutic properties	240:265	the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA)	240:313	Conjugation with the new branched copolymers, PEG-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of L-asparaginase from Erwinia carotovora (EwA).
25754046	4	57	theme	conjugates	695:704	arg1	thermostability					672:686	The thermostability	668:686	The thermostability of EwA conjugates	668:704	The thermostability of EwA conjugates was also considerably improved.
28595071	5	0	theme	contact	971:977	arg1	loadings					934:941	TiO2 loadings	929:941	TiO2 loadings	929:941	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	5	0	theme	contact	971:977	arg1	time					979:982	contact time	971:982	contact time	971:982	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	1	1	theme	green	288:292	arg1	management					294:303	green management	288:303	green management of dye contaminants in wastewater	288:337	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
28595071	0	2	theme	High	94:97	arg1	adsorptivity					99:110	High adsorptivity	94:110	High adsorptivity	94:110	Wastewater remediation by TiO2-impregnated chitosan nano-grafts exhibited dual functionality: High adsorptivity and solar-assisted self-cleaning.
28595071	2	3	theme	TiO2	444:447	arg1	nanocomposites					449:462	chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites	397:462	chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites (Ch-g-PNMANI/TiO2)	397:481	A chitosan-grafted-polyN-Methylaniline (Ch-g-PNMANI) and chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites (Ch-g-PNMANI/TiO2) were prepared and efficiently applied in wastewater remediation.
28595071	2	3	theme	TiO2	444:447	arg1	Ch-g-PNMANI/TiO2					465:480	Ch-g-PNMANI/TiO2	465:480	Ch-g-PNMANI/TiO2	465:480	A chitosan-grafted-polyN-Methylaniline (Ch-g-PNMANI) and chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites (Ch-g-PNMANI/TiO2) were prepared and efficiently applied in wastewater remediation.
28595071	11	4	dep	applied	1745:1751	arg1	enriched					1813:1820	enriched	1813:1820	enriched industrial printing and dyeing wastewater	1813:1862	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	0	5	theme	solar-assisted	116:129	arg1	self-cleaning					131:143	solar-assisted self-cleaning	116:143	solar-assisted self-cleaning	116:143	Wastewater remediation by TiO2-impregnated chitosan nano-grafts exhibited dual functionality: High adsorptivity and solar-assisted self-cleaning.
28595071	6	6	theme	maximum	1033:1039	arg1	adsorption					1041:1050	The maximum adsorption	1029:1050	The maximum adsorption of dye	1029:1057	The maximum adsorption of dye was found at low pH values.
28595071	3	7	theme	diffuse	669:675	arg1	spectroscopy					689:700	UV-Vis diffuse reflectance spectroscopy	662:700	UV-Vis diffuse reflectance spectroscopy	662:700	The nanocomposites were characterized by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy, UV-Vis diffuse reflectance spectroscopy and Brunauer-Emmett-Teller surface area (BET) measurements.
28595071	10	8	theme	photodegradation	1498:1513	arg1	mechanism					1515:1523	a plausible photodegradation mechanism	1486:1523	a plausible photodegradation mechanism	1486:1523	under sunlight irradiation where a plausible photodegradation mechanism was proposed.
28595071	3	9	theme	reflectance	677:687	arg1	spectroscopy					689:700	UV-Vis diffuse reflectance spectroscopy	662:700	UV-Vis diffuse reflectance spectroscopy	662:700	The nanocomposites were characterized by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy, UV-Vis diffuse reflectance spectroscopy and Brunauer-Emmett-Teller surface area (BET) measurements.
28595071	1	10	theme	dye	308:310	arg1	contaminants					312:323	dye contaminants	308:323	dye contaminants	308:323	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
28595071	7	11	contain	has	1145:1147	arg2	116.3mg/g					1177:1185	116.3mg/g	1177:1185	116.3mg/g	1177:1185	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	7	11	contain	has	1145:1147	arg1	Ch-g-PNMANI/TiO2					1100:1115	Ch-g-PNMANI/TiO2	1100:1115	Ch-g-PNMANI/TiO2 of the optimum TiO2 loading	1100:1143	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	7	11	contain	has	1145:1147	arg2	capacity					1167:1174	higher adsorption capacity	1149:1174	higher adsorption capacity (116.3mg/g)	1149:1186	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	3	12	theme	X-ray	609:613	arg1	diffraction					615:625	X-ray diffraction	609:625	X-ray diffraction	609:625	The nanocomposites were characterized by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy, UV-Vis diffuse reflectance spectroscopy and Brunauer-Emmett-Teller surface area (BET) measurements.
28595071	3	13	theme	FT-IR	589:593	arg1	spectroscopy					595:606	FT-IR spectroscopy	589:606	FT-IR spectroscopy	589:606	The nanocomposites were characterized by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy, UV-Vis diffuse reflectance spectroscopy and Brunauer-Emmett-Teller surface area (BET) measurements.
28595071	5	14	theme	dye	995:997	arg1	adsorption					999:1008	dye adsorption	995:1008	dye adsorption	995:1008	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	5	15	theme	TiO2	929:932	arg1	loadings					934:941	TiO2 loadings	929:941	TiO2 loadings	929:941	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	5	15	theme	TiO2	929:932	arg1	concentration					956:968	initial dye concentration	944:968	initial dye concentration	944:968	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	5	15	theme	TiO2	929:932	arg1	time					979:982	contact time	971:982	contact time	971:982	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	5	15	theme	TiO2	929:932	arg1	pH					989:990	pH	989:990	pH	989:990	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	11	16	theme	photoactive	1568:1578	arg1	adsorbents					1580:1589	the presented photoactive adsorbents	1554:1589	the presented photoactive adsorbents	1554:1589	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	11	16	theme	photoactive	1568:1578	arg1	effective					1601:1609	effective	1601:1609	effective	1601:1609	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	11	17	theme	dyeing	1846:1851	arg1	wastewater					1853:1862	dyeing wastewater	1846:1862	dyeing wastewater	1846:1862	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	3	18	theme	UV-Vis	662:667	arg1	spectroscopy					689:700	UV-Vis diffuse reflectance spectroscopy	662:700	UV-Vis diffuse reflectance spectroscopy	662:700	The nanocomposites were characterized by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy, UV-Vis diffuse reflectance spectroscopy and Brunauer-Emmett-Teller surface area (BET) measurements.
28595071	11	19	theme	presented	1558:1566	arg1	adsorbents					1580:1589	the presented photoactive adsorbents	1554:1589	the presented photoactive adsorbents	1554:1589	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	11	19	theme	presented	1558:1566	arg1	effective					1601:1609	effective	1601:1609	effective	1601:1609	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	5	20	theme	initial	944:950	arg1	loadings					934:941	TiO2 loadings	929:941	TiO2 loadings	929:941	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	5	20	theme	initial	944:950	arg1	concentration					956:968	initial dye concentration	944:968	initial dye concentration	944:968	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	7	21	theme	pristine	1197:1204	arg1	108.7mg/g					1219:1227	108.7mg/g	1219:1227	108.7mg/g	1219:1227	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	7	21	theme	pristine	1197:1204	arg1	Ch-g-PNMANI					1206:1216	the pristine Ch-g-PNMANI	1193:1216	the pristine Ch-g-PNMANI (108.7mg/g)	1193:1228	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	3	22	theme	Brunauer-Emmett-Teller	706:727	arg1	measurements					748:759	Brunauer-Emmett-Teller surface area (BET) measurements	706:759	Brunauer-Emmett-Teller surface area (BET) measurements	706:759	The nanocomposites were characterized by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy, UV-Vis diffuse reflectance spectroscopy and Brunauer-Emmett-Teller surface area (BET) measurements.
28595071	11	23	theme	"	1773:1773	arg1	remediation					1788:1798	the rapid, "green" and low-cost remediation	1756:1798	the rapid, "green" and low-cost remediation of RR RB-133	1756:1811	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	4	24	theme	red	834:836	arg1	dye					857:859	remazol red RB-133 (RR RB-133) dye	826:859	remazol red RB-133 (RR RB-133) dye	826:859	The prepared composites exhibit higher adsorptivity in removing remazol red RB-133 (RR RB-133) dye compared to other adsorbents reported in literature.
28595071	0	25	theme	Wastewater	0:9	arg1	remediation					11:21	Wastewater remediation	0:21	Wastewater remediation by TiO2-impregnated chitosan nano-grafts	0:62	Wastewater remediation by TiO2-impregnated chitosan nano-grafts exhibited dual functionality: High adsorptivity and solar-assisted self-cleaning.
28595071	1	26	theme	contaminants	312:323	arg1	management					294:303	green management	288:303	green management of dye contaminants in wastewater	288:337	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
28595071	9	27	theme	fastest	1401:1407	arg1	photoactivity					1423:1435	the fastest self-cleaning photoactivity	1397:1435	the fastest self-cleaning photoactivity	1397:1435	The study addresses a nanocomposite of considerable adsorption and in the same time has the fastest self-cleaning photoactivity (t1/2=31.5min.)
28595071	9	28	theme	considerable	1348:1359	arg1	adsorption					1361:1370	considerable adsorption	1348:1370	considerable adsorption	1348:1370	The study addresses a nanocomposite of considerable adsorption and in the same time has the fastest self-cleaning photoactivity (t1/2=31.5min.)
28595071	3	29	theme	surface	729:735	arg1	measurements					748:759	Brunauer-Emmett-Teller surface area (BET) measurements	706:759	Brunauer-Emmett-Teller surface area (BET) measurements	706:759	The nanocomposites were characterized by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy, UV-Vis diffuse reflectance spectroscopy and Brunauer-Emmett-Teller surface area (BET) measurements.
28595071	10	30	theme	sunlight	1459:1466	arg1	irradiation					1468:1478	sunlight irradiation	1459:1478	sunlight irradiation	1459:1478	under sunlight irradiation where a plausible photodegradation mechanism was proposed.
28595071	1	31	theme	novel	206:210	arg1	composites					244:253	a novel bio-based nanografted polymeric composites	204:253	a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater	204:337	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
28595071	0	32	theme	TiO2-impregnated	26:41	arg1	nano-grafts					52:62	TiO2-impregnated chitosan nano-grafts	26:62	TiO2-impregnated chitosan nano-grafts	26:62	Wastewater remediation by TiO2-impregnated chitosan nano-grafts exhibited dual functionality: High adsorptivity and solar-assisted self-cleaning.
28595071	5	33	theme	dye	952:954	arg1	loadings					934:941	TiO2 loadings	929:941	TiO2 loadings	929:941	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	5	33	theme	dye	952:954	arg1	concentration					956:968	initial dye concentration	944:968	initial dye concentration	944:968	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	9	34	theme	adsorption	1361:1370	arg1	nanocomposite					1331:1343	a nanocomposite	1329:1343	a nanocomposite of considerable adsorption	1329:1370	The study addresses a nanocomposite of considerable adsorption and in the same time has the fastest self-cleaning photoactivity (t1/2=31.5min.)
28595071	9	34	theme	adsorption	1361:1370	arg1	adsorption					1361:1370	considerable adsorption	1348:1370	considerable adsorption	1348:1370	The study addresses a nanocomposite of considerable adsorption and in the same time has the fastest self-cleaning photoactivity (t1/2=31.5min.)
28595071	11	35	theme	adsorption/sunlight-assisted	1638:1665	arg1	cycles					1699:1704	five adsorption/sunlight-assisted self-cleaning photoregeneration cycles	1633:1704	five adsorption/sunlight-assisted self-cleaning photoregeneration cycles	1633:1704	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	2	36	theme	wastewater	524:533	arg1	remediation					535:545	wastewater remediation	524:545	wastewater remediation	524:545	A chitosan-grafted-polyN-Methylaniline (Ch-g-PNMANI) and chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites (Ch-g-PNMANI/TiO2) were prepared and efficiently applied in wastewater remediation.
28595071	4	37	theme	other	873:877	arg1	adsorbents					879:888	other adsorbents	873:888	other adsorbents reported in literature	873:911	The prepared composites exhibit higher adsorptivity in removing remazol red RB-133 (RR RB-133) dye compared to other adsorbents reported in literature.
28595071	7	38	theme	optimum	1124:1130	arg1	loading					1137:1143	the optimum TiO2 loading	1120:1143	the optimum TiO2 loading	1120:1143	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	1	39	theme	unique	187:192	arg1	avenue					194:199	a very infrequent and unique avenue	165:199	a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater	165:337	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
28595071	1	40	theme	nanografted	222:232	arg1	composites					244:253	a novel bio-based nanografted polymeric composites	204:253	a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater	204:337	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
28595071	6	41	theme	pH	1076:1077	arg1	values					1079:1084	low pH values	1072:1084	low pH values	1072:1084	The maximum adsorption of dye was found at low pH values.
28595071	3	42	theme	BET	743:745	arg1	measurements					748:759	Brunauer-Emmett-Teller surface area (BET) measurements	706:759	Brunauer-Emmett-Teller surface area (BET) measurements	706:759	The nanocomposites were characterized by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy, UV-Vis diffuse reflectance spectroscopy and Brunauer-Emmett-Teller surface area (BET) measurements.
28595071	4	43	theme	remazol	826:832	arg1	dye					857:859	remazol red RB-133 (RR RB-133) dye	826:859	remazol red RB-133 (RR RB-133) dye	826:859	The prepared composites exhibit higher adsorptivity in removing remazol red RB-133 (RR RB-133) dye compared to other adsorbents reported in literature.
28595071	1	44	theme	polymeric	234:242	arg1	composites					244:253	a novel bio-based nanografted polymeric composites	204:253	a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater	204:337	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
28595071	0	45	theme	chitosan	43:50	arg1	nano-grafts					52:62	TiO2-impregnated chitosan nano-grafts	26:62	TiO2-impregnated chitosan nano-grafts	26:62	Wastewater remediation by TiO2-impregnated chitosan nano-grafts exhibited dual functionality: High adsorptivity and solar-assisted self-cleaning.
28595071	6	46	theme	low	1072:1074	arg1	values					1079:1084	low pH values	1072:1084	low pH values	1072:1084	The maximum adsorption of dye was found at low pH values.
28595071	1	47	from	management	294:303	arg1	wastewater					328:337	wastewater	328:337	wastewater	328:337	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
28595071	7	48	theme	loading	1137:1143	arg1	Ch-g-PNMANI/TiO2					1100:1115	Ch-g-PNMANI/TiO2	1100:1115	Ch-g-PNMANI/TiO2 of the optimum TiO2 loading	1100:1143	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	1	49	theme	composites	244:253	arg1	avenue					194:199	a very infrequent and unique avenue	165:199	a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater	165:337	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
28595071	0	50	theme	dual	74:77	arg1	functionality					79:91	dual functionality	74:91	dual functionality	74:91	Wastewater remediation by TiO2-impregnated chitosan nano-grafts exhibited dual functionality: High adsorptivity and solar-assisted self-cleaning.
28595071	9	51	theme	same	1383:1386	arg1	time					1388:1391	the same time	1379:1391	the same time	1379:1391	The study addresses a nanocomposite of considerable adsorption and in the same time has the fastest self-cleaning photoactivity (t1/2=31.5min.)
28595071	3	52	theme	area	737:740	arg1	measurements					748:759	Brunauer-Emmett-Teller surface area (BET) measurements	706:759	Brunauer-Emmett-Teller surface area (BET) measurements	706:759	The nanocomposites were characterized by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy, UV-Vis diffuse reflectance spectroscopy and Brunauer-Emmett-Teller surface area (BET) measurements.
28595071	11	53	theme	low-cost	1779:1786	arg1	remediation					1788:1798	the rapid, "green" and low-cost remediation	1756:1798	the rapid, "green" and low-cost remediation of RR RB-133	1756:1811	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	2	54	theme	imprinted	434:442	arg1	nanocomposites					449:462	chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites	397:462	chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites (Ch-g-PNMANI/TiO2)	397:481	A chitosan-grafted-polyN-Methylaniline (Ch-g-PNMANI) and chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites (Ch-g-PNMANI/TiO2) were prepared and efficiently applied in wastewater remediation.
28595071	2	54	theme	imprinted	434:442	arg1	Ch-g-PNMANI/TiO2					465:480	Ch-g-PNMANI/TiO2	465:480	Ch-g-PNMANI/TiO2	465:480	A chitosan-grafted-polyN-Methylaniline (Ch-g-PNMANI) and chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites (Ch-g-PNMANI/TiO2) were prepared and efficiently applied in wastewater remediation.
28595071	11	55	theme	industrial	1822:1831	arg1	printing					1833:1840	industrial printing	1822:1840	industrial printing	1822:1840	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	9	56	theme	self-cleaning	1409:1421	arg1	photoactivity					1423:1435	the fastest self-cleaning photoactivity	1397:1435	the fastest self-cleaning photoactivity	1397:1435	The study addresses a nanocomposite of considerable adsorption and in the same time has the fastest self-cleaning photoactivity (t1/2=31.5min.)
28595071	6	57	located	found	1063:1067	arg1	values					1079:1084	low pH values	1072:1084	low pH values	1072:1084	The maximum adsorption of dye was found at low pH values.
28595071	6	57	located	found	1063:1067	arg2	adsorption					1041:1050	The maximum adsorption	1029:1050	The maximum adsorption of dye	1029:1057	The maximum adsorption of dye was found at low pH values.
28595071	3	58	theme	transmission	628:639	arg1	microscopy					650:659	transmission electron microscopy	628:659	transmission electron microscopy	628:659	The nanocomposites were characterized by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy, UV-Vis diffuse reflectance spectroscopy and Brunauer-Emmett-Teller surface area (BET) measurements.
28595071	2	59	theme	chitosan-grafted-polyN-Methylaniline	397:432	arg1	nanocomposites					449:462	chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites	397:462	chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites (Ch-g-PNMANI/TiO2)	397:481	A chitosan-grafted-polyN-Methylaniline (Ch-g-PNMANI) and chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites (Ch-g-PNMANI/TiO2) were prepared and efficiently applied in wastewater remediation.
28595071	2	59	theme	chitosan-grafted-polyN-Methylaniline	397:432	arg1	Ch-g-PNMANI/TiO2					465:480	Ch-g-PNMANI/TiO2	465:480	Ch-g-PNMANI/TiO2	465:480	A chitosan-grafted-polyN-Methylaniline (Ch-g-PNMANI) and chitosan-grafted-polyN-Methylaniline imprinted TiO2 nanocomposites (Ch-g-PNMANI/TiO2) were prepared and efficiently applied in wastewater remediation.
28595071	9	60	dep	addresses	1319:1327	arg1	has					1393:1395	has	1393:1395	has the fastest self-cleaning photoactivity	1393:1435	The study addresses a nanocomposite of considerable adsorption and in the same time has the fastest self-cleaning photoactivity (t1/2=31.5min.)
28595071	9	60	dep	addresses	1319:1327	arg1	t1/2=31.5min					1438:1449	t1/2=31.5min	1438:1449	t1/2=31.5min	1438:1449	The study addresses a nanocomposite of considerable adsorption and in the same time has the fastest self-cleaning photoactivity (t1/2=31.5min.)
28595071	11	61	theme	rapid	1760:1764	arg1	remediation					1788:1798	the rapid, "green" and low-cost remediation	1756:1798	the rapid, "green" and low-cost remediation of RR RB-133	1756:1811	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	7	62	theme	higher	1149:1154	arg1	capacity					1167:1174	higher adsorption capacity	1149:1174	higher adsorption capacity (116.3mg/g)	1149:1186	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	7	62	theme	higher	1149:1154	arg1	116.3mg/g					1177:1185	116.3mg/g	1177:1185	116.3mg/g	1177:1185	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	5	63	from	effects	918:924	arg1	adsorption					999:1008	dye adsorption	995:1008	dye adsorption	995:1008	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	7	64	theme	TiO2	1132:1135	arg1	loading					1137:1143	the optimum TiO2 loading	1120:1143	the optimum TiO2 loading	1120:1143	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	7	65	theme	adsorption	1156:1165	arg1	capacity					1167:1174	higher adsorption capacity	1149:1174	higher adsorption capacity (116.3mg/g)	1149:1186	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	7	65	theme	adsorption	1156:1165	arg1	116.3mg/g					1177:1185	116.3mg/g	1177:1185	116.3mg/g	1177:1185	Furthermore, Ch-g-PNMANI/TiO2 of the optimum TiO2 loading has higher adsorption capacity (116.3mg/g) than the pristine Ch-g-PNMANI (108.7mg/g).
28595071	10	66	theme	plausible	1488:1496	arg1	mechanism					1515:1523	a plausible photodegradation mechanism	1486:1523	a plausible photodegradation mechanism	1486:1523	under sunlight irradiation where a plausible photodegradation mechanism was proposed.
28595071	11	67	theme	RR	1803:1804	arg1	remediation					1788:1798	the rapid, "green" and low-cost remediation	1756:1798	the rapid, "green" and low-cost remediation of RR RB-133	1756:1811	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	4	68	theme	RB-133	838:843	arg1	dye					857:859	remazol red RB-133 (RR RB-133) dye	826:859	remazol red RB-133 (RR RB-133) dye	826:859	The prepared composites exhibit higher adsorptivity in removing remazol red RB-133 (RR RB-133) dye compared to other adsorbents reported in literature.
28595071	11	69	theme	self-cleaning	1667:1679	arg1	cycles					1699:1704	five adsorption/sunlight-assisted self-cleaning photoregeneration cycles	1633:1704	five adsorption/sunlight-assisted self-cleaning photoregeneration cycles	1633:1704	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	1	70	theme	encouraging	265:275	arg1	results					277:283	encouraging results	265:283	encouraging results	265:283	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
28595071	8	71	theme	prepared	1245:1252	arg1	adsorbents					1254:1263	the prepared adsorbents	1241:1263	the prepared adsorbents	1241:1263	Moreover, the prepared adsorbents are photoactive under sunlight-irradiation.
28595071	4	72	theme	higher	794:799	arg1	adsorptivity					801:812	higher adsorptivity	794:812	higher adsorptivity	794:812	The prepared composites exhibit higher adsorptivity in removing remazol red RB-133 (RR RB-133) dye compared to other adsorbents reported in literature.
28595071	1	73	theme	bio-based	212:220	arg1	composites					244:253	a novel bio-based nanografted polymeric composites	204:253	a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater	204:337	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
28595071	3	74	theme	electron	641:648	arg1	microscopy					650:659	transmission electron microscopy	628:659	transmission electron microscopy	628:659	The nanocomposites were characterized by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy, UV-Vis diffuse reflectance spectroscopy and Brunauer-Emmett-Teller surface area (BET) measurements.
28595071	11	75	theme	photoregeneration	1681:1697	arg1	cycles					1699:1704	five adsorption/sunlight-assisted self-cleaning photoregeneration cycles	1633:1704	five adsorption/sunlight-assisted self-cleaning photoregeneration cycles	1633:1704	Interestingly, the presented photoactive adsorbents are still effective in removing dye after five adsorption/sunlight-assisted self-cleaning photoregeneration cycles and therefore, they can be potentially applied to the rapid, "green" and low-cost remediation of RR RB-133 enriched industrial printing and dyeing wastewater.
28595071	4	76	theme	prepared	766:773	arg1	composites					775:784	The prepared composites	762:784	The prepared composites	762:784	The prepared composites exhibit higher adsorptivity in removing remazol red RB-133 (RR RB-133) dye compared to other adsorbents reported in literature.
28595071	5	77	theme	loadings	934:941	arg1	effects					918:924	The effects	914:924	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption	914:1008	The effects of TiO2 loadings, initial dye concentration, contact time, and pH on dye adsorption were investigated.
28595071	6	78	theme	dye	1055:1057	arg1	adsorption					1041:1050	The maximum adsorption	1029:1050	The maximum adsorption of dye	1029:1057	The maximum adsorption of dye was found at low pH values.
28595071	1	79	theme	infrequent	172:181	arg1	avenue					194:199	a very infrequent and unique avenue	165:199	a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater	165:337	This work provides a very infrequent and unique avenue of a novel bio-based nanografted polymeric composites achieving encouraging results in green management of dye contaminants in wastewater.
27570478	10	0	theme	radiosulfate	1424:1435	arg1	incorporation					1437:1449	radiosulfate incorporation	1424:1449	radiosulfate incorporation	1424:1449	TGFβ, thrombin, PDGF & IGF dose-dependently stimulated radiosulfate incorporation and GAG elongation as well as xyloside-GAG synthesis, however VEGF treatment did not stimulate any changes in proteoglycan synthesis.
27570478	12	1	theme	choroidal	1803:1811	arg1	cells					1825:1829	retinal choroidal endothelial cells	1795:1829	retinal choroidal endothelial cells	1795:1829	Thus, AMD relevant agonists cause glycosaminoglycan hyperelongation of proteoglycans synthesised and secreted by retinal choroidal endothelial cells.
27570478	4	2	theme	endothelial	589:599	arg1	cells					601:605	choroidal endothelial cells	579:605	choroidal endothelial cells	579:605	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	7	3	theme	exchange	1092:1099	arg1	chromatography					1101:1114	ion exchange chromatography	1088:1114	ion exchange chromatography	1088:1114	Proteoglycans were isolated by ion exchange chromatography and sized using SDS-PAGE.
27570478	5	4	theme	oxide	842:846	arg1	markers					815:821	the specific cell markers	797:821	the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor	797:882	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	5	4	theme	oxide	842:846	arg1	synthesis					848:856	endothelial nitric oxide synthesis	823:856	endothelial nitric oxide synthesis	823:856	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	2	5	from	conditions	333:342	arg1	responsible					362:372	responsible	362:372	responsible	362:372	In certain pathological conditions proteoglycans are responsible for lipid retention in tissues.
27570478	4	6	theme	relevant	655:662	arg1	PDGF					695:698	PDGF	695:698	PDGF	695:698	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	6	theme	relevant	655:662	arg1	factors					671:677	AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF	651:712	AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF	651:712	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	6	theme	relevant	655:662	arg1	TGFβ					679:682	TGFβ	679:682	TGFβ	679:682	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	6	theme	relevant	655:662	arg1	IGF					701:703	IGF	701:703	IGF	701:703	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	6	theme	relevant	655:662	arg1	VEGF					709:712	VEGF	709:712	VEGF	709:712	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	6	theme	relevant	655:662	arg1	thrombin					685:692	thrombin	685:692	thrombin	685:692	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	14	7	theme	Future	1945:1950	arg1	studies					1952:1958	Future studies	1945:1958	Future studies	1945:1958	Future studies will examine the relevance of these changes to enhanced lipid binding and the development of AMD.
27570478	8	8	theme	chloride	1207:1214	arg1	technique					1236:1244	the cetylpyridinium chloride (CPC) precipitation technique	1187:1244	the cetylpyridinium chloride (CPC) precipitation technique	1187:1244	Radiosulfate incorporation was determined by the cetylpyridinium chloride (CPC) precipitation technique.
27570478	4	9	from	effects	628:634	arg1	synthesis					730:738	proteoglycan synthesis	717:738	proteoglycan synthesis	717:738	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	5	10	theme	cell	810:813	arg1	markers					815:821	the specific cell markers	797:821	the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor	797:882	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	5	10	theme	cell	810:813	arg1	factor					877:882	von Willebrand factor	862:882	von Willebrand factor	862:882	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	5	10	theme	cell	810:813	arg1	synthesis					848:856	endothelial nitric oxide synthesis	823:856	endothelial nitric oxide synthesis	823:856	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	1	11	from	retina	232:237	arg1	alteration					246:255	an alteration	243:255	an alteration in its composition with increased lipid deposition	243:306	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	11	from	retina	232:237	arg1	feature					131:137	A major feature	123:137	A major feature of early age-related macular degeneration (AMD)	123:185	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	11	from	retina	232:237	arg1	thickening					194:203	the thickening	190:203	the thickening of Bruch's membrane in the retina	190:237	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	12	from	thickening	194:203	arg1	retina					232:237	the retina	228:237	the retina	228:237	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	12	from	thickening	194:203	arg1	composition					264:274	its composition	260:274	its composition with increased lipid deposition	260:306	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	5	13	theme	endothelial	823:833	arg1	oxide					842:846	endothelial nitric oxide	823:846	endothelial nitric oxide synthesis	823:856	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	8	14	theme	precipitation	1222:1234	arg1	technique					1236:1244	the cetylpyridinium chloride (CPC) precipitation technique	1187:1244	the cetylpyridinium chloride (CPC) precipitation technique	1187:1244	Radiosulfate incorporation was determined by the cetylpyridinium chloride (CPC) precipitation technique.
27570478	9	15	theme	cellular	1258:1265	arg1	capacity					1298:1305	cellular glycosaminoglycan synthesizing capacity	1258:1305	cellular glycosaminoglycan synthesizing capacity	1258:1305	To measure cellular glycosaminoglycan synthesizing capacity we added xyloside and assessed the xyloside-GAGs by SDS-PAGE.
27570478	9	16	theme	synthesizing	1285:1296	arg1	capacity					1298:1305	cellular glycosaminoglycan synthesizing capacity	1258:1305	cellular glycosaminoglycan synthesizing capacity	1258:1305	To measure cellular glycosaminoglycan synthesizing capacity we added xyloside and assessed the xyloside-GAGs by SDS-PAGE.
27570478	6	17	theme	appropriate	996:1006	arg1	inhibitors					1008:1017	the appropriate inhibitors	992:1017	the appropriate inhibitors	992:1017	Cells were treated with growth factors in the presence and absence of the appropriate inhibitors and were radiolabeled with [35S]-SO4.
27570478	4	18	theme	factors	671:677	arg1	number					641:646	a number	639:646	a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF	639:712	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	5	19	theme	Willebrand	866:875	arg1	factor					877:882	von Willebrand factor	862:882	von Willebrand factor	862:882	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	5	19	theme	Willebrand	866:875	arg1	markers					815:821	the specific cell markers	797:821	the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor	797:882	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	10	20	theme	xyloside-GAG	1481:1492	arg1	synthesis					1494:1502	xyloside-GAG synthesis	1481:1502	xyloside-GAG synthesis	1481:1502	TGFβ, thrombin, PDGF & IGF dose-dependently stimulated radiosulfate incorporation and GAG elongation as well as xyloside-GAG synthesis, however VEGF treatment did not stimulate any changes in proteoglycan synthesis.
27570478	1	21	theme	macular	160:166	arg1	AMD					182:184	AMD	182:184	AMD	182:184	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	21	theme	macular	160:166	arg1	degeneration					168:179	early age-related macular degeneration	142:179	early age-related macular degeneration (AMD)	142:185	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	12	22	theme	glycosaminoglycan	1716:1732	arg1	hyperelongation					1734:1748	glycosaminoglycan hyperelongation	1716:1748	glycosaminoglycan hyperelongation of proteoglycans synthesised and secreted by retinal choroidal endothelial cells	1716:1829	Thus, AMD relevant agonists cause glycosaminoglycan hyperelongation of proteoglycans synthesised and secreted by retinal choroidal endothelial cells.
27570478	0	23	theme	Retinal	86:92	arg1	Cells					116:120	Retinal Choroidal Endothelial Cells	86:120	Retinal Choroidal Endothelial Cells	86:120	Multiple Growth Factors, But Not VEGF, Stimulate Glycosaminoglycan Hyperelongation in Retinal Choroidal Endothelial Cells.
27570478	10	24	theme	VEGF	1513:1516	arg1	treatment					1518:1526	VEGF treatment	1513:1526	VEGF treatment	1513:1526	TGFβ, thrombin, PDGF & IGF dose-dependently stimulated radiosulfate incorporation and GAG elongation as well as xyloside-GAG synthesis, however VEGF treatment did not stimulate any changes in proteoglycan synthesis.
27570478	0	25	theme	Endothelial	104:114	arg1	Cells					116:120	Retinal Choroidal Endothelial Cells	86:120	Retinal Choroidal Endothelial Cells	86:120	Multiple Growth Factors, But Not VEGF, Stimulate Glycosaminoglycan Hyperelongation in Retinal Choroidal Endothelial Cells.
27570478	12	26	theme	AMD	1688:1690	arg1	agonists					1701:1708	AMD relevant agonists	1688:1708	AMD relevant agonists	1688:1708	Thus, AMD relevant agonists cause glycosaminoglycan hyperelongation of proteoglycans synthesised and secreted by retinal choroidal endothelial cells.
27570478	13	27	theme	potential	1920:1928	arg1	target					1930:1935	a novel potential target	1912:1935	a novel potential target in AMD	1912:1942	The absence of a response to VEGF is intriguing and identifies proteoglycans as a novel potential target in AMD.
27570478	13	27	theme	potential	1920:1928	arg1	proteoglycans					1895:1907	proteoglycans	1895:1907	proteoglycans	1895:1907	The absence of a response to VEGF is intriguing and identifies proteoglycans as a novel potential target in AMD.
27570478	2	28	theme	lipid	378:382	arg1	retention					384:392	lipid retention	378:392	lipid retention in tissues	378:403	In certain pathological conditions proteoglycans are responsible for lipid retention in tissues.
27570478	0	29	theme	Multiple	0:7	arg1	Factors					16:22	Multiple Growth Factors	0:22	Multiple Growth Factors	0:22	Multiple Growth Factors, But Not VEGF, Stimulate Glycosaminoglycan Hyperelongation in Retinal Choroidal Endothelial Cells.
27570478	3	30	theme	Growth	406:411	arg1	factors					413:419	Growth factors	406:419	Growth factors	406:419	Growth factors are known to increase the length of glycosaminoglycan chains and this can lead to a large increase in the interaction between proteoglycans and lipids.
27570478	9	31	dep	added	1310:1314	arg1	measure					1250:1256	measure	1250:1256	To measure cellular glycosaminoglycan synthesizing capacity	1247:1305	To measure cellular glycosaminoglycan synthesizing capacity we added xyloside and assessed the xyloside-GAGs by SDS-PAGE.
27570478	5	32	theme	nitric	835:840	arg1	oxide					842:846	endothelial nitric oxide	823:846	endothelial nitric oxide synthesis	823:856	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	14	33	theme	enhanced	2007:2014	arg1	binding					2022:2028	enhanced lipid binding	2007:2028	enhanced lipid binding	2007:2028	Future studies will examine the relevance of these changes to enhanced lipid binding and the development of AMD.
27570478	10	34	theme	proteoglycan	1561:1572	arg1	synthesis					1574:1582	proteoglycan synthesis	1561:1582	proteoglycan synthesis	1561:1582	TGFβ, thrombin, PDGF & IGF dose-dependently stimulated radiosulfate incorporation and GAG elongation as well as xyloside-GAG synthesis, however VEGF treatment did not stimulate any changes in proteoglycan synthesis.
27570478	2	35	theme	pathological	320:331	arg1	conditions					333:342	certain pathological conditions	312:342	certain pathological conditions	312:342	In certain pathological conditions proteoglycans are responsible for lipid retention in tissues.
27570478	1	36	theme	lipid	291:295	arg1	deposition					297:306	increased lipid deposition	281:306	increased lipid deposition	281:306	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	14	37	theme	changes	1996:2002	arg1	relevance					1977:1985	the relevance	1973:1985	the relevance of these changes to enhanced lipid binding and the development of AMD	1973:2055	Future studies will examine the relevance of these changes to enhanced lipid binding and the development of AMD.
27570478	5	38	dep	markers	815:821	arg1	factor					877:882	von Willebrand factor	862:882	von Willebrand factor	862:882	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	5	38	dep	markers	815:821	arg1	markers					815:821	the specific cell markers	797:821	the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor	797:882	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	5	38	dep	markers	815:821	arg1	synthesis					848:856	endothelial nitric oxide synthesis	823:856	endothelial nitric oxide synthesis	823:856	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	3	39	theme	chains	475:480	arg1	length					447:452	the length	443:452	the length of glycosaminoglycan chains	443:480	Growth factors are known to increase the length of glycosaminoglycan chains and this can lead to a large increase in the interaction between proteoglycans and lipids.
27570478	1	40	theme	early	142:146	arg1	AMD					182:184	AMD	182:184	AMD	182:184	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	40	theme	early	142:146	arg1	degeneration					168:179	early age-related macular degeneration	142:179	early age-related macular degeneration (AMD)	142:185	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	13	41	theme	response	1849:1856	arg1	intriguing					1869:1878	intriguing	1869:1878	intriguing	1869:1878	The absence of a response to VEGF is intriguing and identifies proteoglycans as a novel potential target in AMD.
27570478	13	41	theme	response	1849:1856	arg1	absence					1836:1842	The absence	1832:1842	The absence of a response to VEGF	1832:1864	The absence of a response to VEGF is intriguing and identifies proteoglycans as a novel potential target in AMD.
27570478	10	42	theme	PDGF	1385:1388	arg1	TGFβ					1369:1372	TGFβ	1369:1372	TGFβ	1369:1372	TGFβ, thrombin, PDGF & IGF dose-dependently stimulated radiosulfate incorporation and GAG elongation as well as xyloside-GAG synthesis, however VEGF treatment did not stimulate any changes in proteoglycan synthesis.
27570478	10	42	theme	PDGF	1385:1388	arg1	IGF					1392:1394	PDGF & IGF	1385:1394	PDGF & IGF	1385:1394	TGFβ, thrombin, PDGF & IGF dose-dependently stimulated radiosulfate incorporation and GAG elongation as well as xyloside-GAG synthesis, however VEGF treatment did not stimulate any changes in proteoglycan synthesis.
27570478	1	43	theme	membrane	216:223	arg1	alteration					246:255	an alteration	243:255	an alteration in its composition with increased lipid deposition	243:306	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	43	theme	membrane	216:223	arg1	feature					131:137	A major feature	123:137	A major feature of early age-related macular degeneration (AMD)	123:185	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	43	theme	membrane	216:223	arg1	thickening					194:203	the thickening	190:203	the thickening of Bruch's membrane in the retina	190:237	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	12	44	theme	endothelial	1813:1823	arg1	cells					1825:1829	retinal choroidal endothelial cells	1795:1829	retinal choroidal endothelial cells	1795:1829	Thus, AMD relevant agonists cause glycosaminoglycan hyperelongation of proteoglycans synthesised and secreted by retinal choroidal endothelial cells.
27570478	1	45	with	composition	264:274	arg1	deposition					297:306	increased lipid deposition	281:306	increased lipid deposition	281:306	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	7	46	theme	ion	1088:1090	arg1	chromatography					1101:1114	ion exchange chromatography	1088:1114	ion exchange chromatography	1088:1114	Proteoglycans were isolated by ion exchange chromatography and sized using SDS-PAGE.
27570478	8	47	theme	Radiosulfate	1142:1153	arg1	incorporation					1155:1167	Radiosulfate incorporation	1142:1167	Radiosulfate incorporation	1142:1167	Radiosulfate incorporation was determined by the cetylpyridinium chloride (CPC) precipitation technique.
27570478	4	48	theme	choroidal	579:587	arg1	cells					601:605	choroidal endothelial cells	579:605	choroidal endothelial cells	579:605	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	1	49	theme	degeneration	168:179	arg1	feature					131:137	A major feature	123:137	A major feature of early age-related macular degeneration (AMD)	123:185	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	49	theme	degeneration	168:179	arg1	thickening					194:203	the thickening	190:203	the thickening of Bruch's membrane in the retina	190:237	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	4	50	theme	number	641:646	arg1	effects					628:634	the effects	624:634	the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis	624:738	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	1	51	from	alteration	246:255	arg1	retina					232:237	the retina	228:237	the retina	228:237	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	51	from	alteration	246:255	arg1	composition					264:274	its composition	260:274	its composition with increased lipid deposition	260:306	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	10	52	theme	GAG	1455:1457	arg1	elongation					1459:1468	GAG elongation	1455:1468	GAG elongation	1455:1468	TGFβ, thrombin, PDGF & IGF dose-dependently stimulated radiosulfate incorporation and GAG elongation as well as xyloside-GAG synthesis, however VEGF treatment did not stimulate any changes in proteoglycan synthesis.
27570478	2	53	from	responsible	362:372	arg1	conditions					333:342	certain pathological conditions	312:342	certain pathological conditions	312:342	In certain pathological conditions proteoglycans are responsible for lipid retention in tissues.
27570478	4	54	theme	AMD	651:653	arg1	PDGF					695:698	PDGF	695:698	PDGF	695:698	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	54	theme	AMD	651:653	arg1	factors					671:677	AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF	651:712	AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF	651:712	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	54	theme	AMD	651:653	arg1	TGFβ					679:682	TGFβ	679:682	TGFβ	679:682	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	54	theme	AMD	651:653	arg1	IGF					701:703	IGF	701:703	IGF	701:703	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	54	theme	AMD	651:653	arg1	VEGF					709:712	VEGF	709:712	VEGF	709:712	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	54	theme	AMD	651:653	arg1	thrombin					685:692	thrombin	685:692	thrombin	685:692	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	12	55	theme	retinal	1795:1801	arg1	cells					1825:1829	retinal choroidal endothelial cells	1795:1829	retinal choroidal endothelial cells	1795:1829	Thus, AMD relevant agonists cause glycosaminoglycan hyperelongation of proteoglycans synthesised and secreted by retinal choroidal endothelial cells.
27570478	5	56	theme	specific	801:808	arg1	markers					815:821	the specific cell markers	797:821	the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor	797:882	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	5	56	theme	specific	801:808	arg1	factor					877:882	von Willebrand factor	862:882	von Willebrand factor	862:882	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	5	56	theme	specific	801:808	arg1	synthesis					848:856	endothelial nitric oxide synthesis	823:856	endothelial nitric oxide synthesis	823:856	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	8	57	theme	cetylpyridinium	1191:1205	arg1	CPC					1217:1219	CPC	1217:1219	CPC	1217:1219	Radiosulfate incorporation was determined by the cetylpyridinium chloride (CPC) precipitation technique.
27570478	8	57	theme	cetylpyridinium	1191:1205	arg1	chloride					1207:1214	cetylpyridinium chloride	1191:1214	the cetylpyridinium chloride (CPC) precipitation technique	1187:1244	Radiosulfate incorporation was determined by the cetylpyridinium chloride (CPC) precipitation technique.
27570478	6	58	theme	growth	946:951	arg1	factors					953:959	growth factors	946:959	growth factors	946:959	Cells were treated with growth factors in the presence and absence of the appropriate inhibitors and were radiolabeled with [35S]-SO4.
27570478	14	59	theme	AMD	2053:2055	arg1	binding					2022:2028	enhanced lipid binding	2007:2028	enhanced lipid binding	2007:2028	Future studies will examine the relevance of these changes to enhanced lipid binding and the development of AMD.
27570478	14	59	theme	AMD	2053:2055	arg1	development					2038:2048	the development	2034:2048	the development of AMD	2034:2055	Future studies will examine the relevance of these changes to enhanced lipid binding and the development of AMD.
27570478	1	60	from	membrane	216:223	arg1	retina					232:237	the retina	228:237	the retina	228:237	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	9	61	theme	glycosaminoglycan	1267:1283	arg1	capacity					1298:1305	cellular glycosaminoglycan synthesizing capacity	1258:1305	cellular glycosaminoglycan synthesizing capacity	1258:1305	To measure cellular glycosaminoglycan synthesizing capacity we added xyloside and assessed the xyloside-GAGs by SDS-PAGE.
27570478	1	62	theme	major	125:129	arg1	feature					131:137	A major feature	123:137	A major feature of early age-related macular degeneration (AMD)	123:185	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	62	theme	major	125:129	arg1	thickening					194:203	the thickening	190:203	the thickening of Bruch's membrane in the retina	190:237	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	6	63	theme	inhibitors	1008:1017	arg1	absence					981:987	absence	981:987	absence	981:987	Cells were treated with growth factors in the presence and absence of the appropriate inhibitors and were radiolabeled with [35S]-SO4.
27570478	6	63	theme	inhibitors	1008:1017	arg1	presence					968:975	presence	968:975	presence	968:975	Cells were treated with growth factors in the presence and absence of the appropriate inhibitors and were radiolabeled with [35S]-SO4.
27570478	0	64	theme	Glycosaminoglycan	49:65	arg1	Hyperelongation					67:81	Glycosaminoglycan Hyperelongation	49:81	Glycosaminoglycan Hyperelongation	49:81	Multiple Growth Factors, But Not VEGF, Stimulate Glycosaminoglycan Hyperelongation in Retinal Choroidal Endothelial Cells.
27570478	5	65	theme	von	862:864	arg1	factor					877:882	von Willebrand factor	862:882	von Willebrand factor	862:882	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	5	65	theme	von	862:864	arg1	markers					815:821	the specific cell markers	797:821	the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor	797:882	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	12	66	theme	proteoglycans	1753:1765	arg1	hyperelongation					1734:1748	glycosaminoglycan hyperelongation	1716:1748	glycosaminoglycan hyperelongation of proteoglycans synthesised and secreted by retinal choroidal endothelial cells	1716:1829	Thus, AMD relevant agonists cause glycosaminoglycan hyperelongation of proteoglycans synthesised and secreted by retinal choroidal endothelial cells.
27570478	6	67	dep	presence	968:975	arg1	the					964:966	the	964:966	the	964:966	Cells were treated with growth factors in the presence and absence of the appropriate inhibitors and were radiolabeled with [35S]-SO4.
27570478	4	68	theme	growth	664:669	arg1	PDGF					695:698	PDGF	695:698	PDGF	695:698	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	68	theme	growth	664:669	arg1	factors					671:677	AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF	651:712	AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF	651:712	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	68	theme	growth	664:669	arg1	TGFβ					679:682	TGFβ	679:682	TGFβ	679:682	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	68	theme	growth	664:669	arg1	IGF					701:703	IGF	701:703	IGF	701:703	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	68	theme	growth	664:669	arg1	VEGF					709:712	VEGF	709:712	VEGF	709:712	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	68	theme	growth	664:669	arg1	thrombin					685:692	thrombin	685:692	thrombin	685:692	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	3	69	from	increase	511:518	arg1	interaction					527:537	the interaction	523:537	the interaction between proteoglycans and lipids	523:570	Growth factors are known to increase the length of glycosaminoglycan chains and this can lead to a large increase in the interaction between proteoglycans and lipids.
27570478	1	70	theme	age-related	148:158	arg1	AMD					182:184	AMD	182:184	AMD	182:184	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	1	70	theme	age-related	148:158	arg1	degeneration					168:179	early age-related macular degeneration	142:179	early age-related macular degeneration (AMD)	142:185	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	12	71	theme	relevant	1692:1699	arg1	agonists					1701:1708	AMD relevant agonists	1688:1708	AMD relevant agonists	1688:1708	Thus, AMD relevant agonists cause glycosaminoglycan hyperelongation of proteoglycans synthesised and secreted by retinal choroidal endothelial cells.
27570478	10	72	from	changes	1550:1556	arg1	synthesis					1574:1582	proteoglycan synthesis	1561:1582	proteoglycan synthesis	1561:1582	TGFβ, thrombin, PDGF & IGF dose-dependently stimulated radiosulfate incorporation and GAG elongation as well as xyloside-GAG synthesis, however VEGF treatment did not stimulate any changes in proteoglycan synthesis.
27570478	0	73	theme	Choroidal	94:102	arg1	Cells					116:120	Retinal Choroidal Endothelial Cells	86:120	Retinal Choroidal Endothelial Cells	86:120	Multiple Growth Factors, But Not VEGF, Stimulate Glycosaminoglycan Hyperelongation in Retinal Choroidal Endothelial Cells.
27570478	4	74	theme	proteoglycan	717:728	arg1	synthesis					730:738	proteoglycan synthesis	717:738	proteoglycan synthesis	717:738	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	75	dep	factors	671:677	arg1	PDGF					695:698	PDGF	695:698	PDGF	695:698	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	75	dep	factors	671:677	arg1	factors					671:677	AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF	651:712	AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF	651:712	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	75	dep	factors	671:677	arg1	TGFβ					679:682	TGFβ	679:682	TGFβ	679:682	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	75	dep	factors	671:677	arg1	IGF					701:703	IGF	701:703	IGF	701:703	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	75	dep	factors	671:677	arg1	VEGF					709:712	VEGF	709:712	VEGF	709:712	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	4	75	dep	factors	671:677	arg1	thrombin					685:692	thrombin	685:692	thrombin	685:692	Using choroidal endothelial cells, we investigated the effects of a number of AMD relevant growth factors TGFβ, thrombin, PDGF, IGF and VEGF on proteoglycan synthesis.
27570478	5	76	theme	confocal	901:908	arg1	microscopy					910:919	confocal microscopy	901:919	confocal microscopy	901:919	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	13	77	theme	novel	1914:1918	arg1	target					1930:1935	a novel potential target	1912:1935	a novel potential target in AMD	1912:1942	The absence of a response to VEGF is intriguing and identifies proteoglycans as a novel potential target in AMD.
27570478	13	77	theme	novel	1914:1918	arg1	proteoglycans					1895:1907	proteoglycans	1895:1907	proteoglycans	1895:1907	The absence of a response to VEGF is intriguing and identifies proteoglycans as a novel potential target in AMD.
27570478	5	78	theme	endothelial	772:782	arg1	origin					784:789	endothelial origin	772:789	endothelial origin	772:789	Cells were characterized as of endothelial origin using the specific cell markers endothelial nitric oxide synthesis and von Willebrand factor and imaged using confocal microscopy.
27570478	12	79	dep	proteoglycans	1753:1765	arg1	secreted					1783:1790	secreted	1783:1790	secreted by retinal choroidal endothelial cells	1783:1829	Thus, AMD relevant agonists cause glycosaminoglycan hyperelongation of proteoglycans synthesised and secreted by retinal choroidal endothelial cells.
27570478	12	79	dep	proteoglycans	1753:1765	arg1	synthesised					1767:1777	synthesised	1767:1777	synthesised	1767:1777	Thus, AMD relevant agonists cause glycosaminoglycan hyperelongation of proteoglycans synthesised and secreted by retinal choroidal endothelial cells.
27570478	13	80	from	target	1930:1935	arg1	AMD					1940:1942	AMD	1940:1942	AMD	1940:1942	The absence of a response to VEGF is intriguing and identifies proteoglycans as a novel potential target in AMD.
27570478	3	81	theme	glycosaminoglycan	457:473	arg1	chains					475:480	glycosaminoglycan chains	457:480	glycosaminoglycan chains	457:480	Growth factors are known to increase the length of glycosaminoglycan chains and this can lead to a large increase in the interaction between proteoglycans and lipids.
27570478	14	82	theme	lipid	2016:2020	arg1	binding					2022:2028	enhanced lipid binding	2007:2028	enhanced lipid binding	2007:2028	Future studies will examine the relevance of these changes to enhanced lipid binding and the development of AMD.
27570478	11	83	theme	large	1625:1629	arg1	increase					1631:1638	a large increase	1623:1638	a large increase in pERK	1623:1646	VEGF did not increase pAKT but caused a large increase in pERK relative to the response to PDGF.
27570478	1	84	theme	increased	281:289	arg1	deposition					297:306	increased lipid deposition	281:306	increased lipid deposition	281:306	A major feature of early age-related macular degeneration (AMD) is the thickening of Bruch's membrane in the retina and an alteration in its composition with increased lipid deposition.
27570478	2	85	theme	certain	312:318	arg1	conditions					333:342	certain pathological conditions	312:342	certain pathological conditions	312:342	In certain pathological conditions proteoglycans are responsible for lipid retention in tissues.
27570478	2	86	from	retention	384:392	arg1	tissues					397:403	tissues	397:403	tissues	397:403	In certain pathological conditions proteoglycans are responsible for lipid retention in tissues.
27570478	10	87	theme	&	1390:1390	arg1	TGFβ					1369:1372	TGFβ	1369:1372	TGFβ	1369:1372	TGFβ, thrombin, PDGF & IGF dose-dependently stimulated radiosulfate incorporation and GAG elongation as well as xyloside-GAG synthesis, however VEGF treatment did not stimulate any changes in proteoglycan synthesis.
27570478	10	87	theme	&	1390:1390	arg1	IGF					1392:1394	PDGF & IGF	1385:1394	PDGF & IGF	1385:1394	TGFβ, thrombin, PDGF & IGF dose-dependently stimulated radiosulfate incorporation and GAG elongation as well as xyloside-GAG synthesis, however VEGF treatment did not stimulate any changes in proteoglycan synthesis.
27570478	3	88	theme	large	505:509	arg1	increase					511:518	a large increase	503:518	a large increase in the interaction between proteoglycans and lipids	503:570	Growth factors are known to increase the length of glycosaminoglycan chains and this can lead to a large increase in the interaction between proteoglycans and lipids.
27570478	11	89	from	increase	1631:1638	arg1	pERK					1643:1646	pERK	1643:1646	pERK	1643:1646	VEGF did not increase pAKT but caused a large increase in pERK relative to the response to PDGF.
27570478	0	90	theme	Growth	9:14	arg1	Factors					16:22	Multiple Growth Factors	0:22	Multiple Growth Factors	0:22	Multiple Growth Factors, But Not VEGF, Stimulate Glycosaminoglycan Hyperelongation in Retinal Choroidal Endothelial Cells.
25575585	0	0	theme	food	78:81	arg1	waste					83:87	food waste	78:87	food waste	78:87	Using feature objects aided strategy to evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	1	1	theme	corn	236:239	arg1	co-digestion					257:268	corn stalk anaerobic co-digestion	236:268	corn stalk anaerobic co-digestion	236:268	Feature objects aided strategy was used to predict and evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	5	2	theme	corn	767:770	arg1	mono-digestion					778:791	corn stalk mono-digestion	767:791	corn stalk mono-digestion	767:791	Food waste and corn stalk co-digestion enhanced the digestion rate and achieved 22.48% and 41.55% higher biomethane production than those of food waste and corn stalk mono-digestion, respectively.
25575585	4	3	theme	objects	514:520	arg1	digestion					487:495	The anaerobic digestion	473:495	The anaerobic digestion of three feature objects at different mixing ratios	473:547	The anaerobic digestion of three feature objects at different mixing ratios showed the different biomethane yields and kinetic constants.
25575585	5	4	theme	digestion	663:671	arg1	rate					673:676	the digestion rate	659:676	the digestion rate	659:676	Food waste and corn stalk co-digestion enhanced the digestion rate and achieved 22.48% and 41.55% higher biomethane production than those of food waste and corn stalk mono-digestion, respectively.
25575585	2	5	theme	stalk	345:349	arg1	mono-digestion					304:317	mono-digestion	304:317	mono-digestion	304:317	The kinetics of co-digestion and mono-digestion of food waste and/or corn stalk was also analyzed.
25575585	2	5	theme	stalk	345:349	arg1	co-digestion					287:298	co-digestion	287:298	co-digestion	287:298	The kinetics of co-digestion and mono-digestion of food waste and/or corn stalk was also analyzed.
25575585	1	6	theme	stalk	241:245	arg1	co-digestion					257:268	corn stalk anaerobic co-digestion	236:268	corn stalk anaerobic co-digestion	236:268	Feature objects aided strategy was used to predict and evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	4	7	theme	feature	506:512	arg1	objects					514:520	three feature objects	500:520	three feature objects	500:520	The anaerobic digestion of three feature objects at different mixing ratios showed the different biomethane yields and kinetic constants.
25575585	5	8	theme	Food	611:614	arg1	waste					616:620	Food waste and corn stalk co-digestion	611:648	waste	616:620	Food waste and corn stalk co-digestion enhanced the digestion rate and achieved 22.48% and 41.55% higher biomethane production than those of food waste and corn stalk mono-digestion, respectively.
25575585	1	9	theme	anaerobic	247:255	arg1	co-digestion					257:268	corn stalk anaerobic co-digestion	236:268	corn stalk anaerobic co-digestion	236:268	Feature objects aided strategy was used to predict and evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	0	10	theme	waste	83:87	arg1	production					64:73	the biomethane production	49:73	the biomethane production of food waste and corn stalk anaerobic co-digestion	49:125	Using feature objects aided strategy to evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	1	11	theme	co-digestion	257:268	arg1	production					207:216	the biomethane production	192:216	the biomethane production of food waste and corn stalk anaerobic co-digestion	192:268	Feature objects aided strategy was used to predict and evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	0	12	theme	stalk	98:102	arg1	co-digestion					114:125	corn stalk anaerobic co-digestion	93:125	corn stalk anaerobic co-digestion	93:125	Using feature objects aided strategy to evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	5	13	theme	corn	626:629	arg1	co-digestion					637:648	Food waste and corn stalk co-digestion	611:648	co-digestion	637:648	Food waste and corn stalk co-digestion enhanced the digestion rate and achieved 22.48% and 41.55% higher biomethane production than those of food waste and corn stalk mono-digestion, respectively.
25575585	4	14	theme	kinetic	592:598	arg1	constants					600:608	kinetic constants	592:608	kinetic constants	592:608	The anaerobic digestion of three feature objects at different mixing ratios showed the different biomethane yields and kinetic constants.
25575585	0	15	theme	corn	93:96	arg1	co-digestion					114:125	corn stalk anaerobic co-digestion	93:125	corn stalk anaerobic co-digestion	93:125	Using feature objects aided strategy to evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	5	16	theme	stalk	772:776	arg1	mono-digestion					778:791	corn stalk mono-digestion	767:791	corn stalk mono-digestion	767:791	Food waste and corn stalk co-digestion enhanced the digestion rate and achieved 22.48% and 41.55% higher biomethane production than those of food waste and corn stalk mono-digestion, respectively.
25575585	3	17	theme	corn	432:435	arg1	stalk					437:441	corn stalk	432:441	corn stalk	432:441	The results indicated that the compositions of food waste and corn stalk were significantly different.
25575585	0	18	theme	feature	6:12	arg1	objects					14:20	feature objects	6:20	feature objects	6:20	Using feature objects aided strategy to evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	4	19	theme	anaerobic	477:485	arg1	digestion					487:495	The anaerobic digestion	473:495	The anaerobic digestion of three feature objects at different mixing ratios	473:547	The anaerobic digestion of three feature objects at different mixing ratios showed the different biomethane yields and kinetic constants.
25575585	5	20	dep	higher	709:714	arg1	production					727:736	biomethane production	716:736	biomethane production	716:736	Food waste and corn stalk co-digestion enhanced the digestion rate and achieved 22.48% and 41.55% higher biomethane production than those of food waste and corn stalk mono-digestion, respectively.
25575585	3	21	theme	food	417:420	arg1	waste					422:426	food waste	417:426	food waste	417:426	The results indicated that the compositions of food waste and corn stalk were significantly different.
25575585	2	22	theme	mono-digestion	304:317	arg1	kinetics					275:282	The kinetics	271:282	The kinetics of co-digestion and mono-digestion of food waste and/or corn stalk	271:349	The kinetics of co-digestion and mono-digestion of food waste and/or corn stalk was also analyzed.
25575585	3	23	theme	waste	422:426	arg1	compositions					401:412	the compositions	397:412	the compositions of food waste and corn stalk	397:441	The results indicated that the compositions of food waste and corn stalk were significantly different.
25575585	3	23	theme	waste	422:426	arg1	different					462:470	different	462:470	different	462:470	The results indicated that the compositions of food waste and corn stalk were significantly different.
25575585	0	24	theme	co-digestion	114:125	arg1	production					64:73	the biomethane production	49:73	the biomethane production of food waste and corn stalk anaerobic co-digestion	49:125	Using feature objects aided strategy to evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	1	25	dep	objects	136:142	arg1	aided					144:148	aided	144:148	objects aided strategy was used to predict and evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion	136:268	Feature objects aided strategy was used to predict and evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	4	26	theme	biomethane	570:579	arg1	yields					581:586	the different biomethane yields	556:586	the different biomethane yields	556:586	The anaerobic digestion of three feature objects at different mixing ratios showed the different biomethane yields and kinetic constants.
25575585	0	27	theme	anaerobic	104:112	arg1	co-digestion					114:125	corn stalk anaerobic co-digestion	93:125	corn stalk anaerobic co-digestion	93:125	Using feature objects aided strategy to evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	2	28	theme	co-digestion	287:298	arg1	kinetics					275:282	The kinetics	271:282	The kinetics of co-digestion and mono-digestion of food waste and/or corn stalk	271:349	The kinetics of co-digestion and mono-digestion of food waste and/or corn stalk was also analyzed.
25575585	4	29	from	ratios	542:547	arg1	digestion					487:495	The anaerobic digestion	473:495	The anaerobic digestion of three feature objects at different mixing ratios	473:547	The anaerobic digestion of three feature objects at different mixing ratios showed the different biomethane yields and kinetic constants.
25575585	4	30	theme	different	560:568	arg1	yields					581:586	the different biomethane yields	556:586	the different biomethane yields	556:586	The anaerobic digestion of three feature objects at different mixing ratios showed the different biomethane yields and kinetic constants.
25575585	5	31	theme	higher	709:714	arg1	%					707:707	41.55%	702:707	41.55% higher biomethane production than those of food waste and corn stalk mono-digestion	702:791	Food waste and corn stalk co-digestion enhanced the digestion rate and achieved 22.48% and 41.55% higher biomethane production than those of food waste and corn stalk mono-digestion, respectively.
25575585	3	32	theme	stalk	437:441	arg1	compositions					401:412	the compositions	397:412	the compositions of food waste and corn stalk	397:441	The results indicated that the compositions of food waste and corn stalk were significantly different.
25575585	3	32	theme	stalk	437:441	arg1	different					462:470	different	462:470	different	462:470	The results indicated that the compositions of food waste and corn stalk were significantly different.
25575585	1	33	theme	biomethane	196:205	arg1	production					207:216	the biomethane production	192:216	the biomethane production of food waste and corn stalk anaerobic co-digestion	192:268	Feature objects aided strategy was used to predict and evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	4	34	theme	different	525:533	arg1	ratios					542:547	different mixing ratios	525:547	different mixing ratios	525:547	The anaerobic digestion of three feature objects at different mixing ratios showed the different biomethane yields and kinetic constants.
25575585	5	35	theme	biomethane	716:725	arg1	production					727:736	biomethane production	716:736	biomethane production	716:736	Food waste and corn stalk co-digestion enhanced the digestion rate and achieved 22.48% and 41.55% higher biomethane production than those of food waste and corn stalk mono-digestion, respectively.
25575585	2	36	theme	corn	340:343	arg1	stalk					345:349	corn stalk	340:349	corn stalk	340:349	The kinetics of co-digestion and mono-digestion of food waste and/or corn stalk was also analyzed.
25575585	5	37	theme	stalk	631:635	arg1	co-digestion					637:648	Food waste and corn stalk co-digestion	611:648	co-digestion	637:648	Food waste and corn stalk co-digestion enhanced the digestion rate and achieved 22.48% and 41.55% higher biomethane production than those of food waste and corn stalk mono-digestion, respectively.
25575585	4	38	theme	mixing	535:540	arg1	ratios					542:547	different mixing ratios	525:547	different mixing ratios	525:547	The anaerobic digestion of three feature objects at different mixing ratios showed the different biomethane yields and kinetic constants.
25575585	2	39	theme	waste	327:331	arg1	mono-digestion					304:317	mono-digestion	304:317	mono-digestion	304:317	The kinetics of co-digestion and mono-digestion of food waste and/or corn stalk was also analyzed.
25575585	2	39	theme	waste	327:331	arg1	co-digestion					287:298	co-digestion	287:298	co-digestion	287:298	The kinetics of co-digestion and mono-digestion of food waste and/or corn stalk was also analyzed.
25575585	2	40	theme	food	322:325	arg1	waste					327:331	food waste	322:331	food waste	322:331	The kinetics of co-digestion and mono-digestion of food waste and/or corn stalk was also analyzed.
25575585	1	41	theme	food	221:224	arg1	waste					226:230	food waste	221:230	food waste	221:230	Feature objects aided strategy was used to predict and evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	5	42	theme	food	752:755	arg1	waste					757:761	food waste	752:761	food waste	752:761	Food waste and corn stalk co-digestion enhanced the digestion rate and achieved 22.48% and 41.55% higher biomethane production than those of food waste and corn stalk mono-digestion, respectively.
25575585	1	43	theme	waste	226:230	arg1	production					207:216	the biomethane production	192:216	the biomethane production of food waste and corn stalk anaerobic co-digestion	192:268	Feature objects aided strategy was used to predict and evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	0	44	theme	biomethane	53:62	arg1	production					64:73	the biomethane production	49:73	the biomethane production of food waste and corn stalk anaerobic co-digestion	49:125	Using feature objects aided strategy to evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
25575585	1	45	used	used	163:166	arg2	strategy					150:157	strategy	150:157	strategy	150:157	Feature objects aided strategy was used to predict and evaluate the biomethane production of food waste and corn stalk anaerobic co-digestion.
27987980	8	0	with	experiments	1043:1053	arg1	cells					1076:1080	osteoblast-like cells	1060:1080	osteoblast-like cells	1060:1080	Cell culture experiments with osteoblast-like cells on the scaffolds demonstrated that incorporation of PCL nanofibers into the fabricated scaffolds improves cell viability and attachment.
27987980	0	1	theme	chitosan-polycaprolactone-calcium	26:58	arg1	phosphate					60:68	Novel chitosan-sulfonated chitosan-polycaprolactone-calcium phosphate	0:68	Novel chitosan-sulfonated chitosan-polycaprolactone-calcium phosphate	0:68	Novel chitosan-sulfonated chitosan-polycaprolactone-calcium phosphate nanocomposite scaffold.
27987980	4	2	theme	phosphate	652:660	arg1	solutions					667:675	calcium and phosphate rich solutions	640:675	calcium and phosphate rich solutions	640:675	In the end, apatite formed on/in the composite scaffolds through alternate dipping in calcium and phosphate rich solutions.
27987980	5	3	theme	mechanical	723:732	arg1	properties					734:743	mechanical properties	723:743	mechanical properties of the scaffolds	723:760	The morphology, apatite forming ability, and mechanical properties of the scaffolds were evaluated.
27987980	1	4	from	nanofibers	193:202	arg1	scaffolds					122:130	New bioactive nanocomposite scaffolds	94:130	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers	94:202	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers were prepared.
27987980	8	5	theme	culture	1035:1041	arg1	experiments					1043:1053	Cell culture experiments	1030:1053	Cell culture experiments with osteoblast-like cells on the scaffolds	1030:1097	Cell culture experiments with osteoblast-like cells on the scaffolds demonstrated that incorporation of PCL nanofibers into the fabricated scaffolds improves cell viability and attachment.
27987980	1	6	theme	bioactive	98:106	arg1	scaffolds					122:130	New bioactive nanocomposite scaffolds	94:130	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers	94:202	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers were prepared.
27987980	7	7	theme	mechanical	1007:1016	arg1	properties					1018:1027	the mechanical properties	1003:1027	the mechanical properties	1003:1027	Contrary to the effects of increased pore size, addition of PCL nanofibers enhanced the mechanical properties.
27987980	1	8	theme	nanocomposite	108:120	arg1	scaffolds					122:130	New bioactive nanocomposite scaffolds	94:130	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers	94:202	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers were prepared.
27987980	4	9	theme	rich	662:665	arg1	solutions					667:675	calcium and phosphate rich solutions	640:675	calcium and phosphate rich solutions	640:675	In the end, apatite formed on/in the composite scaffolds through alternate dipping in calcium and phosphate rich solutions.
27987980	5	10	theme	apatite	694:700	arg1	ability					710:716	apatite forming ability	694:716	apatite forming ability	694:716	The morphology, apatite forming ability, and mechanical properties of the scaffolds were evaluated.
27987980	7	11	theme	pore	956:959	arg1	size					961:964	increased pore size	946:964	increased pore size	946:964	Contrary to the effects of increased pore size, addition of PCL nanofibers enhanced the mechanical properties.
27987980	8	12	theme	Cell	1030:1033	arg1	experiments					1043:1053	Cell culture experiments	1030:1053	Cell culture experiments with osteoblast-like cells on the scaffolds	1030:1097	Cell culture experiments with osteoblast-like cells on the scaffolds demonstrated that incorporation of PCL nanofibers into the fabricated scaffolds improves cell viability and attachment.
27987980	7	13	theme	size	961:964	arg1	effects					935:941	the effects	931:941	the effects of increased pore size	931:964	Contrary to the effects of increased pore size, addition of PCL nanofibers enhanced the mechanical properties.
27987980	5	14	theme	forming	702:708	arg1	ability					710:716	apatite forming ability	694:716	apatite forming ability	694:716	The morphology, apatite forming ability, and mechanical properties of the scaffolds were evaluated.
27987980	0	15	theme	chitosan-sulfonated	6:24	arg1	phosphate					60:68	Novel chitosan-sulfonated chitosan-polycaprolactone-calcium phosphate	0:68	Novel chitosan-sulfonated chitosan-polycaprolactone-calcium phosphate	0:68	Novel chitosan-sulfonated chitosan-polycaprolactone-calcium phosphate nanocomposite scaffold.
27987980	0	16	theme	Novel	0:4	arg1	phosphate					60:68	Novel chitosan-sulfonated chitosan-polycaprolactone-calcium phosphate	0:68	Novel chitosan-sulfonated chitosan-polycaprolactone-calcium phosphate	0:68	Novel chitosan-sulfonated chitosan-polycaprolactone-calcium phosphate nanocomposite scaffold.
27987980	7	17	theme	PCL	979:981	arg1	nanofibers					983:992	PCL nanofibers	979:992	PCL nanofibers	979:992	Contrary to the effects of increased pore size, addition of PCL nanofibers enhanced the mechanical properties.
27987980	8	18	theme	nanofibers	1138:1147	arg1	incorporation					1117:1129	incorporation	1117:1129	incorporation of PCL nanofibers into the fabricated scaffolds	1117:1177	Cell culture experiments with osteoblast-like cells on the scaffolds demonstrated that incorporation of PCL nanofibers into the fabricated scaffolds improves cell viability and attachment.
27987980	3	19	theme	chopped-hydrophilic	417:435	arg1	nanofibers					441:450	chopped-hydrophilic PCL nanofibers	417:450	chopped-hydrophilic PCL nanofibers	417:450	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers were incorporated into the fabricated scaffold in order to mimic extracellular matrix-like structure.
27987980	4	20	theme	alternate	619:627	arg1	dipping					629:635	alternate dipping	619:635	alternate dipping in calcium and phosphate rich solutions	619:675	In the end, apatite formed on/in the composite scaffolds through alternate dipping in calcium and phosphate rich solutions.
27987980	6	21	theme	apatite	844:850	arg1	sizes					817:821	larger pore sizes	805:821	larger pore sizes	805:821	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	6	21	theme	apatite	844:850	arg1	deposition					852:861	apatite deposition	844:861	apatite deposition	844:861	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	1	22	theme	sulfonated	147:156	arg1	chitosan					158:165	sulfonated chitosan	147:165	sulfonated chitosan	147:165	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers were prepared.
27987980	3	23	theme	matrix-like	531:541	arg1	structure					543:551	extracellular matrix-like structure	517:551	extracellular matrix-like structure	517:551	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers were incorporated into the fabricated scaffold in order to mimic extracellular matrix-like structure.
27987980	3	24	theme	PCL	437:439	arg1	nanofibers					441:450	chopped-hydrophilic PCL nanofibers	417:450	chopped-hydrophilic PCL nanofibers	417:450	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers were incorporated into the fabricated scaffold in order to mimic extracellular matrix-like structure.
27987980	4	25	theme	calcium	640:646	arg1	solutions					667:675	calcium and phosphate rich solutions	640:675	calcium and phosphate rich solutions	640:675	In the end, apatite formed on/in the composite scaffolds through alternate dipping in calcium and phosphate rich solutions.
27987980	8	26	theme	fabricated	1158:1167	arg1	scaffolds					1169:1177	the fabricated scaffolds	1154:1177	the fabricated scaffolds	1154:1177	Cell culture experiments with osteoblast-like cells on the scaffolds demonstrated that incorporation of PCL nanofibers into the fabricated scaffolds improves cell viability and attachment.
27987980	8	27	dep	cell	1188:1191	arg1	viability					1193:1201	viability	1193:1201	viability	1193:1201	Cell culture experiments with osteoblast-like cells on the scaffolds demonstrated that incorporation of PCL nanofibers into the fabricated scaffolds improves cell viability and attachment.
27987980	8	28	from	experiments	1043:1053	arg1	scaffolds					1089:1097	the scaffolds	1085:1097	the scaffolds	1085:1097	Cell culture experiments with osteoblast-like cells on the scaffolds demonstrated that incorporation of PCL nanofibers into the fabricated scaffolds improves cell viability and attachment.
27987980	2	29	theme	chlorosulfonic	273:286	arg1	acid					288:291	chlorosulfonic acid	273:291	chlorosulfonic acid	273:291	In this study, sulfonated chitosan was prepared using chlorosulfonic acid and combined with chitosan in order to improve the bioactivity of chitosan-based scaffolds.
27987980	3	30	theme	fabricated	479:488	arg1	scaffold					490:497	the fabricated scaffold	475:497	the fabricated scaffold	475:497	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers were incorporated into the fabricated scaffold in order to mimic extracellular matrix-like structure.
27987980	4	31	theme	composite	591:599	arg1	scaffolds					601:609	the composite scaffolds	587:609	the composite scaffolds	587:609	In the end, apatite formed on/in the composite scaffolds through alternate dipping in calcium and phosphate rich solutions.
27987980	3	32	dep	Meanwhile	385:393	arg1	different					396:404	different	396:404	different	396:404	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers were incorporated into the fabricated scaffold in order to mimic extracellular matrix-like structure.
27987980	4	33	from	dipping	629:635	arg1	solutions					667:675	calcium and phosphate rich solutions	640:675	calcium and phosphate rich solutions	640:675	In the end, apatite formed on/in the composite scaffolds through alternate dipping in calcium and phosphate rich solutions.
27987980	3	34	theme	extracellular	517:529	arg1	structure					543:551	extracellular matrix-like structure	517:551	extracellular matrix-like structure	517:551	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers were incorporated into the fabricated scaffold in order to mimic extracellular matrix-like structure.
27987980	5	35	theme	scaffolds	752:760	arg1	ability					710:716	apatite forming ability	694:716	apatite forming ability	694:716	The morphology, apatite forming ability, and mechanical properties of the scaffolds were evaluated.
27987980	5	35	theme	scaffolds	752:760	arg1	properties					734:743	mechanical properties	723:743	mechanical properties of the scaffolds	723:760	The morphology, apatite forming ability, and mechanical properties of the scaffolds were evaluated.
27987980	5	35	theme	scaffolds	752:760	arg1	morphology					682:691	The morphology	678:691	The morphology	678:691	The morphology, apatite forming ability, and mechanical properties of the scaffolds were evaluated.
27987980	6	36	theme	PCL	903:905	arg1	nanofibers					907:916	more PCL nanofibers	898:916	more PCL nanofibers	898:916	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	6	37	theme	higher	824:829	arg1	sizes					817:821	larger pore sizes	805:821	larger pore sizes	805:821	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	6	37	theme	higher	824:829	arg1	porosity					831:838	higher porosity	824:838	higher porosity	824:838	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	8	38	theme	PCL	1134:1136	arg1	nanofibers					1138:1147	PCL nanofibers	1134:1147	PCL nanofibers	1134:1147	Cell culture experiments with osteoblast-like cells on the scaffolds demonstrated that incorporation of PCL nanofibers into the fabricated scaffolds improves cell viability and attachment.
27987980	7	39	theme	nanofibers	983:992	arg1	addition					967:974	addition	967:974	addition of PCL nanofibers	967:992	Contrary to the effects of increased pore size, addition of PCL nanofibers enhanced the mechanical properties.
27987980	2	40	theme	sulfonated	234:243	arg1	chitosan					245:252	sulfonated chitosan	234:252	sulfonated chitosan	234:252	In this study, sulfonated chitosan was prepared using chlorosulfonic acid and combined with chitosan in order to improve the bioactivity of chitosan-based scaffolds.
27987980	8	41	theme	osteoblast-like	1060:1074	arg1	cells					1076:1080	osteoblast-like cells	1060:1080	osteoblast-like cells	1060:1080	Cell culture experiments with osteoblast-like cells on the scaffolds demonstrated that incorporation of PCL nanofibers into the fabricated scaffolds improves cell viability and attachment.
27987980	1	42	theme	poly	172:175	arg1	nanofibers					193:202	poly(ε-caprolactone) nanofibers	172:202	poly(ε-caprolactone) nanofibers	172:202	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers were prepared.
27987980	1	43	from	chitosan	158:165	arg1	scaffolds					122:130	New bioactive nanocomposite scaffolds	94:130	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers	94:202	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers were prepared.
27987980	6	44	theme	more	898:901	arg1	nanofibers					907:916	more PCL nanofibers	898:916	more PCL nanofibers	898:916	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	2	45	theme	chitosan-based	359:372	arg1	scaffolds					374:382	chitosan-based scaffolds	359:382	chitosan-based scaffolds	359:382	In this study, sulfonated chitosan was prepared using chlorosulfonic acid and combined with chitosan in order to improve the bioactivity of chitosan-based scaffolds.
27987980	1	46	from	chitosan	137:144	arg1	scaffolds					122:130	New bioactive nanocomposite scaffolds	94:130	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers	94:202	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers were prepared.
27987980	2	47	theme	scaffolds	374:382	arg1	bioactivity					344:354	the bioactivity	340:354	the bioactivity of chitosan-based scaffolds	340:382	In this study, sulfonated chitosan was prepared using chlorosulfonic acid and combined with chitosan in order to improve the bioactivity of chitosan-based scaffolds.
27987980	6	48	theme	pore	812:815	arg1	porosity					831:838	higher porosity	824:838	higher porosity	824:838	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	6	48	theme	pore	812:815	arg1	sizes					817:821	larger pore sizes	805:821	larger pore sizes	805:821	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	6	48	theme	pore	812:815	arg1	result					881:886	a result	879:886	a result of adding more PCL nanofibers	879:916	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	6	48	theme	pore	812:815	arg1	deposition					852:861	apatite deposition	844:861	apatite deposition	844:861	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	3	49	theme	Meanwhile	385:393	arg1	nanofibers					441:450	chopped-hydrophilic PCL nanofibers	417:450	chopped-hydrophilic PCL nanofibers	417:450	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers were incorporated into the fabricated scaffold in order to mimic extracellular matrix-like structure.
27987980	3	49	theme	Meanwhile	385:393	arg1	amounts					406:412	Meanwhile, different amounts	385:412	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers	385:450	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers were incorporated into the fabricated scaffold in order to mimic extracellular matrix-like structure.
27987980	1	50	theme	New	94:96	arg1	scaffolds					122:130	New bioactive nanocomposite scaffolds	94:130	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers	94:202	New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers were prepared.
27987980	6	51	theme	larger	805:810	arg1	porosity					831:838	higher porosity	824:838	higher porosity	824:838	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	6	51	theme	larger	805:810	arg1	sizes					817:821	larger pore sizes	805:821	larger pore sizes	805:821	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	6	51	theme	larger	805:810	arg1	result					881:886	a result	879:886	a result of adding more PCL nanofibers	879:916	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	6	51	theme	larger	805:810	arg1	deposition					852:861	apatite deposition	844:861	apatite deposition	844:861	The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers.
27987980	3	52	theme	nanofibers	441:450	arg1	nanofibers					441:450	chopped-hydrophilic PCL nanofibers	417:450	chopped-hydrophilic PCL nanofibers	417:450	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers were incorporated into the fabricated scaffold in order to mimic extracellular matrix-like structure.
27987980	3	52	theme	nanofibers	441:450	arg1	amounts					406:412	Meanwhile, different amounts	385:412	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers	385:450	Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers were incorporated into the fabricated scaffold in order to mimic extracellular matrix-like structure.
27987980	7	53	theme	increased	946:954	arg1	size					961:964	increased pore size	946:964	increased pore size	946:964	Contrary to the effects of increased pore size, addition of PCL nanofibers enhanced the mechanical properties.
24793896	7	0	from	6h	1347:1348	arg1	%					1359:1359	80-95%	1354:1359	80-95% at 12h	1354:1366	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	0	from	6h	1347:1348	arg1	%					1342:1342	40-65%	1337:1342	40-65% at 6h	1337:1348	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	6	1	theme	EE	1140:1141	arg1	effect					1129:1134	the effect	1125:1134	the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets	1125:1244	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	3	2	theme	exvivo	702:707	arg1	bioadhesion					709:719	exvivo bioadhesion	702:719	exvivo bioadhesion	702:719	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	2	theme	exvivo	702:707	arg1	characteristics					635:649	suitable quality characteristics	618:649	suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	618:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	8	3	theme	38.18	1781:1785	arg1	ng					1787:1788	ng	1787:1788	ng	1787:1788	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	7	4	theme	granisetron	1431:1441	arg1	F19					1456:1458	F19	1456:1458	F19	1456:1458	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	4	theme	granisetron	1431:1441	arg1	formulation					1443:1453	in vivo adhesion time (>14 h) granisetron formulation	1401:1453	in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC	1401:1483	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	1	5	theme	cancer	265:270	arg1	chemotherapy					272:283	cancer chemotherapy	265:283	cancer chemotherapy	265:283	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	7	6	theme	h	1428:1428	arg1	F19					1456:1458	F19	1456:1458	F19	1456:1458	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	6	theme	h	1428:1428	arg1	formulation					1443:1453	in vivo adhesion time (>14 h) granisetron formulation	1401:1453	in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC	1401:1483	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	4	7	theme	cholesterol	882:892	arg1	concentration					894:906	cholesterol concentration	882:906	cholesterol concentration	882:906	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	7	8	dep	F19	1456:1458	arg1	mg					1465:1466	1.4 mg	1461:1466	1.4 mg	1461:1466	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	3	9	theme	granisetron	535:545	arg1	bioavailability					516:530	bioavailability	516:530	bioavailability of granisetron	516:545	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	7	10	theme	behavior	1387:1394	arg1	achievement					1273:1283	achievement	1273:1283	achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC	1273:1483	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	8	11	theme	oral	1810:1813	arg1	formulation					1815:1825	buccal and oral formulation	1799:1825	formulation	1815:1825	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	2	12	theme	oral	319:322	arg1	%					344:344	≈60%	341:344	≈60%	341:344	Granisetron suffers from reduced oral bioavailability (≈60%) due to hepatic metabolism.
24793896	2	12	theme	oral	319:322	arg1	bioavailability					324:338	reduced oral bioavailability	311:338	reduced oral bioavailability (≈60%) due to hepatic metabolism	311:371	Granisetron suffers from reduced oral bioavailability (≈60%) due to hepatic metabolism.
24793896	7	13	dep	in	1370:1371	arg1	vivo					1373:1376	vivo	1373:1376	vivo	1373:1376	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	4	14	theme	vesicle	968:974	arg1	size					976:979	vesicle size	968:979	vesicle size	968:979	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	6	15	theme	drug	1184:1187	arg1	release					1189:1195	in vitro drug release	1175:1195	in vitro drug release	1175:1195	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	8	16	dep	granisetron	1748:1758	arg1	h/ml					1790:1793	38.18 ng h/ml	1781:1793	38.18 ng h/ml for buccal and oral formulation	1781:1825	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	8	16	dep	granisetron	1748:1758	arg1	89.97					1771:1775	89.97	1771:1775	89.97	1771:1775	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	3	17	theme	granisetron	566:576	arg1	tablets					605:611	granisetron provesicular buccoadhesive tablets	566:611	granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	566:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	8	18	theme	higher	1658:1663	arg1	bioavailability					1665:1679	higher bioavailability	1658:1679	higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively)	1658:1840	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	7	19	dep	in	1401:1402	arg1	vivo					1404:1407	vivo	1404:1407	vivo	1404:1407	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	1	20	from	emesis	255:260	arg1	hydrochloride					146:158	Granisetron hydrochloride	134:158	Granisetron hydrochloride (granisetron)	134:172	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	1	20	from	emesis	255:260	arg1	chemotherapy					272:283	cancer chemotherapy	265:283	cancer chemotherapy	265:283	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	1	20	from	emesis	255:260	arg1	effective					224:232	effective	224:232	effective	224:232	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	1	20	from	emesis	255:260	arg1	antiemetic					186:195	a potent antiemetic	177:195	a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy	177:283	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	3	21	theme	quality	627:633	arg1	behavior					745:752	in vivo bioadhesion behavior	725:752	in vivo bioadhesion behavior	725:752	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	21	theme	quality	627:633	arg1	content					667:673	drug content	662:673	drug content	662:673	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	21	theme	quality	627:633	arg1	bioadhesion					709:719	exvivo bioadhesion	702:719	exvivo bioadhesion	702:719	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	21	theme	quality	627:633	arg1	characteristics					635:649	suitable quality characteristics	618:649	suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	618:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	21	theme	quality	627:633	arg1	hardness					652:659	hardness	652:659	hardness	652:659	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	21	theme	quality	627:633	arg1	pattern					693:699	in vitro release pattern	676:699	in vitro release pattern	676:699	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	4	22	theme	%	959:959	arg1	EE					960:961	%EE	959:961	%EE	959:961	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	4	22	theme	%	959:959	arg1	efficiency					947:956	entrapment efficiency	936:956	entrapment efficiency (%EE)	936:962	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	10	23	theme	granisetron	2064:2074	arg1	F19					2077:2079	granisetron (F19)	2064:2080	granisetron (F19)	2064:2080	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	8	24	theme	formulation	1691:1701	arg1	bioavailability					1665:1679	higher bioavailability	1658:1679	higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively)	1658:1840	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	1	25	theme	Granisetron	134:144	arg1	hydrochloride					146:158	Granisetron hydrochloride	134:158	Granisetron hydrochloride (granisetron)	134:172	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	1	25	theme	Granisetron	134:144	arg1	effective					224:232	effective	224:232	effective	224:232	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	1	25	theme	Granisetron	134:144	arg1	antiemetic					186:195	a potent antiemetic	177:195	a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy	177:283	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	1	25	theme	Granisetron	134:144	arg1	granisetron					161:171	granisetron	161:171	granisetron	161:171	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	7	26	dep	in	1297:1298	arg1	vitro					1300:1304	vitro	1300:1304	vitro	1300:1304	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	6	27	theme	tablets	1238:1244	arg1	release					1189:1195	in vitro drug release	1175:1195	in vitro drug release	1175:1195	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	6	27	theme	tablets	1238:1244	arg1	performance					1209:1219	in vivo performance	1201:1219	in vivo performance of buccoadhesive tablets	1201:1244	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	8	28	theme	oral	1728:1731	arg1	formulation					1733:1743	conventional oral formulation	1715:1743	conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively)	1715:1840	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	5	29	attach	derived	1097:1103	arg2	those					1091:1095	those	1091:1095	those	1091:1095	Span 80 proniosome-derived niosomes exhibited higher encapsulation efficiency and smaller particle size than those derived from span 20.
24793896	5	29	attach	derived	1097:1103	arg1	span					1110:1113	span 20	1110:1116	span 20	1110:1116	Span 80 proniosome-derived niosomes exhibited higher encapsulation efficiency and smaller particle size than those derived from span 20.
24793896	10	30	theme	F19	2077:2079	arg1	tablet					2054:2059	The prepared provesicular buccoadhesive tablet	2014:2059	The prepared provesicular buccoadhesive tablet of granisetron (F19)	2014:2080	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	0	31	theme	preliminary	84:94	arg1	investigation					96:108	preliminary investigation	84:108	preliminary investigation of in vivo performance	84:131	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	31	theme	preliminary	84:94	arg1	formulations					46:57	Provesicular granisetron hydrochloride buccal formulations	0:57	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.	0:132	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	10	32	theme	granisetron	2161:2171	arg1	bioavailability					2142:2156	bioavailability	2142:2156	bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency	2142:2260	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	10	33	theme	first-pass	2108:2117	arg1	metabolism					2119:2128	hepatic first-pass metabolism	2100:2128	hepatic first-pass metabolism	2100:2128	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	4	34	theme	niosomes	789:796	arg1	Composition					756:766	Composition	756:766	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration	756:906	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	7	35	theme	required	1288:1295	arg1	pattern					1314:1320	required in vitro release pattern	1288:1320	required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h)	1288:1367	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	5	36	theme	higher	1028:1033	arg1	efficiency					1049:1058	higher encapsulation efficiency	1028:1058	higher encapsulation efficiency	1028:1058	Span 80 proniosome-derived niosomes exhibited higher encapsulation efficiency and smaller particle size than those derived from span 20.
24793896	7	37	theme	in	1604:1605	arg1	study					1612:1616	in vivo study	1604:1616	in vivo study	1604:1616	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	4	38	theme	different	803:811	arg1	carriers					835:842	different prepared provesicular carriers	803:842	different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration	803:906	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	10	39	with	bioavailability	2142:2156	arg1	possibility					2182:2192	the possibility	2178:2192	the possibility of reducing reported daily dose (2mg) and reducing dosing frequency	2178:2260	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	9	40	from	8h	1904:1905	arg1	ng/ml					1894:1898	12.09±4.47 ng/ml	1883:1898	12.09±4.47 ng/ml	1883:1898	A significantly lower and delayed Cmax (12.09±4.47 ng/ml, at 8h) was observed after buccal application compared to conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h).
24793896	8	41	dep	In	1619:1620	arg1	vivo					1622:1625	vivo	1622:1625	vivo	1622:1625	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	3	42	theme	provesicular	414:425	arg1	carriers					427:434	provesicular carriers	414:434	provesicular carriers	414:434	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	0	43	theme	Provesicular	0:11	arg1	evaluation					69:78	in vitro evaluation	60:78	in vitro evaluation	60:78	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	43	theme	Provesicular	0:11	arg1	investigation					96:108	preliminary investigation	84:108	preliminary investigation of in vivo performance	84:131	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	43	theme	Provesicular	0:11	arg1	formulations					46:57	Provesicular granisetron hydrochloride buccal formulations	0:57	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.	0:132	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	4	44	from	Composition	756:766	arg1	carriers					835:842	different prepared provesicular carriers	803:842	different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration	803:906	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	10	45	theme	daily	2215:2219	arg1	2mg					2227:2229	2mg	2227:2229	2mg	2227:2229	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	10	45	theme	daily	2215:2219	arg1	dose					2221:2224	reported daily dose	2206:2224	reported daily dose (2mg)	2206:2230	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	7	46	from	12h	1364:1366	arg1	%					1359:1359	80-95%	1354:1359	80-95% at 12h	1354:1366	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	46	from	12h	1364:1366	arg1	%					1342:1342	40-65%	1337:1342	40-65% at 6h	1337:1348	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	9	47	theme	oral	1971:1974	arg1	tablet					1976:1981	conventional oral tablet	1958:1981	conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h)	1958:2011	A significantly lower and delayed Cmax (12.09±4.47 ng/ml, at 8h) was observed after buccal application compared to conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h).
24793896	0	48	theme	hydrochloride	25:37	arg1	evaluation					69:78	in vitro evaluation	60:78	in vitro evaluation	60:78	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	48	theme	hydrochloride	25:37	arg1	investigation					96:108	preliminary investigation	84:108	preliminary investigation of in vivo performance	84:131	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	48	theme	hydrochloride	25:37	arg1	formulations					46:57	Provesicular granisetron hydrochloride buccal formulations	0:57	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.	0:132	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	3	49	theme	in	676:677	arg1	pattern					693:699	in vitro release pattern	676:699	in vitro release pattern	676:699	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	49	theme	in	676:677	arg1	characteristics					635:649	suitable quality characteristics	618:649	suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	618:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	50	theme	drug	447:450	arg1	delivery					452:459	buccal drug delivery	440:459	buccal drug delivery	440:459	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	0	51	dep	in	60:61	arg1	vitro					63:67	vitro	63:67	vitro	63:67	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	52	theme	in	60:61	arg1	evaluation					69:78	in vitro evaluation	60:78	in vitro evaluation	60:78	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	52	theme	in	60:61	arg1	formulations					46:57	Provesicular granisetron hydrochloride buccal formulations	0:57	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.	0:132	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	53	dep	formulations	46:57	arg1	evaluation					69:78	in vitro evaluation	60:78	in vitro evaluation	60:78	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	53	dep	formulations	46:57	arg1	investigation					96:108	preliminary investigation	84:108	preliminary investigation of in vivo performance	84:131	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	53	dep	formulations	46:57	arg1	formulations					46:57	Provesicular granisetron hydrochloride buccal formulations	0:57	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.	0:132	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	7	54	from	2h	1333:1334	arg1	%					1328:1328	20-30%	1323:1328	20-30% at 2h	1323:1334	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	4	55	theme	provesicular	822:833	arg1	carriers					835:842	different prepared provesicular carriers	803:842	different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration	803:906	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	8	56	theme	relative	1703:1710	arg1	formulation					1691:1701	buccal formulation	1684:1701	buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively)	1684:1840	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	7	57	theme	in	1401:1402	arg1	F19					1456:1458	F19	1456:1458	F19	1456:1458	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	57	theme	in	1401:1402	arg1	formulation					1443:1453	in vivo adhesion time (>14 h) granisetron formulation	1401:1453	in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC	1401:1483	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	5	58	theme	smaller	1064:1070	arg1	size					1081:1084	smaller particle size	1064:1084	smaller particle size	1064:1084	Span 80 proniosome-derived niosomes exhibited higher encapsulation efficiency and smaller particle size than those derived from span 20.
24793896	7	59	theme	pattern	1314:1320	arg1	achievement					1273:1283	achievement	1273:1283	achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC	1273:1483	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	3	60	theme	release	685:691	arg1	pattern					693:699	in vitro release pattern	676:699	in vitro release pattern	676:699	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	60	theme	release	685:691	arg1	characteristics					635:649	suitable quality characteristics	618:649	suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	618:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	6	61	dep	in	1175:1176	arg1	vitro					1178:1182	vitro	1178:1182	vitro	1178:1182	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	7	62	theme	formulation	1443:1453	arg1	achievement					1273:1283	achievement	1273:1283	achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC	1273:1483	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	8	63	theme	buccal	1799:1804	arg1	formulation					1815:1825	buccal and oral formulation	1799:1825	formulation	1815:1825	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	3	64	theme	in	725:726	arg1	behavior					745:752	in vivo bioadhesion behavior	725:752	in vivo bioadhesion behavior	725:752	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	64	theme	in	725:726	arg1	characteristics					635:649	suitable quality characteristics	618:649	suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	618:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	8	65	theme	ng	1787:1788	arg1	h/ml					1790:1793	38.18 ng h/ml	1781:1793	38.18 ng h/ml for buccal and oral formulation	1781:1825	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	1	66	theme	delayed	247:253	arg1	emesis					255:260	delayed emesis	247:260	delayed emesis in cancer chemotherapy	247:283	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	6	67	theme	%	1139:1139	arg1	EE					1140:1141	%EE	1139:1141	%EE	1139:1141	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	7	68	theme	>14	1424:1426	arg1	F19					1456:1458	F19	1456:1458	F19	1456:1458	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	68	theme	>14	1424:1426	arg1	formulation					1443:1453	in vivo adhesion time (>14 h) granisetron formulation	1401:1453	in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC	1401:1483	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	4	69	theme	entrapment	936:945	arg1	EE					960:961	%EE	959:961	%EE	959:961	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	4	69	theme	entrapment	936:945	arg1	efficiency					947:956	entrapment efficiency	936:956	entrapment efficiency (%EE)	936:962	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	7	70	theme	adhesion	1409:1416	arg1	F19					1456:1458	F19	1456:1458	F19	1456:1458	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	70	theme	adhesion	1409:1416	arg1	formulation					1443:1453	in vivo adhesion time (>14 h) granisetron formulation	1401:1453	in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC	1401:1483	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	9	71	dep	Cmax	1877:1880	arg1	ng/ml					1894:1898	12.09±4.47 ng/ml	1883:1898	12.09±4.47 ng/ml	1883:1898	A significantly lower and delayed Cmax (12.09±4.47 ng/ml, at 8h) was observed after buccal application compared to conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h).
24793896	6	72	from	effect	1129:1134	arg1	release					1189:1195	in vitro drug release	1175:1195	in vitro drug release	1175:1195	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	6	72	from	effect	1129:1134	arg1	performance					1209:1219	in vivo performance	1201:1219	in vivo performance of buccoadhesive tablets	1201:1244	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	1	73	theme	potent	179:184	arg1	hydrochloride					146:158	Granisetron hydrochloride	134:158	Granisetron hydrochloride (granisetron)	134:172	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	1	73	theme	potent	179:184	arg1	effective					224:232	effective	224:232	effective	224:232	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	1	73	theme	potent	179:184	arg1	antiemetic					186:195	a potent antiemetic	177:195	a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy	177:283	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	9	74	from	h	2010:2010	arg1	ng/ml					1996:2000	31.66±10.15 ng/ml	1984:2000	31.66±10.15 ng/ml	1984:2000	A significantly lower and delayed Cmax (12.09±4.47 ng/ml, at 8h) was observed after buccal application compared to conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h).
24793896	7	75	theme	time	1418:1421	arg1	F19					1456:1458	F19	1456:1458	F19	1456:1458	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	75	theme	time	1418:1421	arg1	formulation					1443:1453	in vivo adhesion time (>14 h) granisetron formulation	1401:1453	in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC	1401:1483	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	10	76	theme	reported	2206:2213	arg1	2mg					2227:2229	2mg	2227:2229	2mg	2227:2229	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	10	76	theme	reported	2206:2213	arg1	dose					2221:2224	reported daily dose	2206:2224	reported daily dose (2mg)	2206:2230	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	6	77	theme	in	1201:1202	arg1	performance					1209:1219	in vivo performance	1201:1219	in vivo performance of buccoadhesive tablets	1201:1244	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	3	78	theme	bioadhesion	733:743	arg1	behavior					745:752	in vivo bioadhesion behavior	725:752	in vivo bioadhesion behavior	725:752	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	78	theme	bioadhesion	733:743	arg1	characteristics					635:649	suitable quality characteristics	618:649	suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	618:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	79	theme	provesicular	578:589	arg1	tablets					605:611	granisetron provesicular buccoadhesive tablets	566:611	granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	566:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	8	80	theme	In	1619:1620	arg1	study					1643:1647	In vivo pharmacokinetic study	1619:1647	In vivo pharmacokinetic study	1619:1647	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	10	81	theme	buccoadhesive	2040:2052	arg1	tablet					2054:2059	The prepared provesicular buccoadhesive tablet	2014:2059	The prepared provesicular buccoadhesive tablet of granisetron (F19)	2014:2080	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	2	82	theme	reduced	311:317	arg1	%					344:344	≈60%	341:344	≈60%	341:344	Granisetron suffers from reduced oral bioavailability (≈60%) due to hepatic metabolism.
24793896	2	82	theme	reduced	311:317	arg1	bioavailability					324:338	reduced oral bioavailability	311:338	reduced oral bioavailability (≈60%) due to hepatic metabolism	311:371	Granisetron suffers from reduced oral bioavailability (≈60%) due to hepatic metabolism.
24793896	8	83	theme	pharmacokinetic	1627:1641	arg1	study					1643:1647	In vivo pharmacokinetic study	1619:1647	In vivo pharmacokinetic study	1619:1647	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	3	84	dep	characteristics	635:649	arg1	behavior					745:752	in vivo bioadhesion behavior	725:752	in vivo bioadhesion behavior	725:752	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	84	dep	characteristics	635:649	arg1	content					667:673	drug content	662:673	drug content	662:673	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	84	dep	characteristics	635:649	arg1	bioadhesion					709:719	exvivo bioadhesion	702:719	exvivo bioadhesion	702:719	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	84	dep	characteristics	635:649	arg1	characteristics					635:649	suitable quality characteristics	618:649	suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	618:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	84	dep	characteristics	635:649	arg1	hardness					652:659	hardness	652:659	hardness	652:659	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	84	dep	characteristics	635:649	arg1	pattern					693:699	in vitro release pattern	676:699	in vitro release pattern	676:699	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	85	theme	buccoadhesive	591:603	arg1	tablets					605:611	granisetron provesicular buccoadhesive tablets	566:611	granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	566:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	7	86	theme	swelling	1378:1385	arg1	behavior					1387:1394	in vivo swelling behavior	1370:1394	in vivo swelling behavior	1370:1394	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	9	87	theme	lower	1859:1863	arg1	Cmax					1877:1880	A significantly lower and delayed Cmax	1843:1880	A significantly lower and delayed Cmax (12.09±4.47 ng/ml, at 8h)	1843:1906	A significantly lower and delayed Cmax (12.09±4.47 ng/ml, at 8h) was observed after buccal application compared to conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h).
24793896	6	88	dep	in	1201:1202	arg1	vivo					1204:1207	vivo	1204:1207	vivo	1204:1207	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	3	89	theme	suitable	618:625	arg1	behavior					745:752	in vivo bioadhesion behavior	725:752	in vivo bioadhesion behavior	725:752	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	89	theme	suitable	618:625	arg1	content					667:673	drug content	662:673	drug content	662:673	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	89	theme	suitable	618:625	arg1	bioadhesion					709:719	exvivo bioadhesion	702:719	exvivo bioadhesion	702:719	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	89	theme	suitable	618:625	arg1	characteristics					635:649	suitable quality characteristics	618:649	suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	618:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	89	theme	suitable	618:625	arg1	hardness					652:659	hardness	652:659	hardness	652:659	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	89	theme	suitable	618:625	arg1	pattern					693:699	in vitro release pattern	676:699	in vitro release pattern	676:699	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	7	90	theme	vesicular	1538:1546	arg1	precursors					1548:1557	the vesicular precursors span 80 and cholesterol (9:1)	1534:1587	the vesicular precursors span 80 and cholesterol (9:1)	1534:1587	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	90	theme	vesicular	1538:1546	arg1	span					1559:1562	span 80	1559:1565	span 80	1559:1565	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	90	theme	vesicular	1538:1546	arg1	cholesterol					1571:1581	cholesterol	1571:1581	cholesterol (9:1)	1571:1587	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	91	theme	in	1370:1371	arg1	behavior					1387:1394	in vivo swelling behavior	1370:1394	in vivo swelling behavior	1370:1394	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	9	92	theme	delayed	1869:1875	arg1	Cmax					1877:1880	A significantly lower and delayed Cmax	1843:1880	A significantly lower and delayed Cmax (12.09±4.47 ng/ml, at 8h)	1843:1906	A significantly lower and delayed Cmax (12.09±4.47 ng/ml, at 8h) was observed after buccal application compared to conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h).
24793896	3	93	with	tablets	605:611	arg1	behavior					745:752	in vivo bioadhesion behavior	725:752	in vivo bioadhesion behavior	725:752	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	93	with	tablets	605:611	arg1	content					667:673	drug content	662:673	drug content	662:673	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	93	with	tablets	605:611	arg1	bioadhesion					709:719	exvivo bioadhesion	702:719	exvivo bioadhesion	702:719	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	93	with	tablets	605:611	arg1	characteristics					635:649	suitable quality characteristics	618:649	suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	618:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	93	with	tablets	605:611	arg1	hardness					652:659	hardness	652:659	hardness	652:659	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	93	with	tablets	605:611	arg1	pattern					693:699	in vitro release pattern	676:699	in vitro release pattern	676:699	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	1	94	from	acute	237:241	arg1	hydrochloride					146:158	Granisetron hydrochloride	134:158	Granisetron hydrochloride (granisetron)	134:172	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	1	94	from	acute	237:241	arg1	effective					224:232	effective	224:232	effective	224:232	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	1	94	from	acute	237:241	arg1	antiemetic					186:195	a potent antiemetic	177:195	a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy	177:283	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	3	95	theme	tablets	605:611	arg1	development					551:561	development	551:561	development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	551:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	10	96	theme	dosing	2245:2250	arg1	frequency					2252:2260	dosing frequency	2245:2260	dosing frequency	2245:2260	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	7	97	dep	in	1604:1605	arg1	vivo					1607:1610	vivo	1607:1610	vivo	1607:1610	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	1	98	from	effective	224:232	arg1	acute					237:241	acute	237:241	acute	237:241	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	1	98	from	effective	224:232	arg1	emesis					255:260	delayed emesis	247:260	delayed emesis in cancer chemotherapy	247:283	Granisetron hydrochloride (granisetron) is a potent antiemetic that has been proven to be effective in acute and delayed emesis in cancer chemotherapy.
24793896	8	99	theme	buccal	1684:1689	arg1	formulation					1691:1701	buccal formulation	1684:1701	buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively)	1684:1840	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	10	100	theme	prepared	2018:2025	arg1	tablet					2054:2059	The prepared provesicular buccoadhesive tablet	2014:2059	The prepared provesicular buccoadhesive tablet of granisetron (F19)	2014:2080	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	9	101	dep	tablet	1976:1981	arg1	ng/ml					1996:2000	31.66±10.15 ng/ml	1984:2000	31.66±10.15 ng/ml	1984:2000	A significantly lower and delayed Cmax (12.09±4.47 ng/ml, at 8h) was observed after buccal application compared to conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h).
24793896	10	102	theme	provesicular	2027:2038	arg1	tablet					2054:2059	The prepared provesicular buccoadhesive tablet	2014:2059	The prepared provesicular buccoadhesive tablet of granisetron (F19)	2014:2080	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	7	103	theme	release	1306:1312	arg1	pattern					1314:1320	required in vitro release pattern	1288:1320	required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h)	1288:1367	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	4	104	from	carriers	835:842	arg1	niosomes					789:796	the reconstituted niosomes	771:796	the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration	771:906	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	4	104	from	carriers	835:842	arg1	Composition					756:766	Composition	756:766	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration	756:906	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	6	105	theme	buccoadhesive	1224:1236	arg1	tablets					1238:1244	buccoadhesive tablets	1224:1244	buccoadhesive tablets	1224:1244	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	8	106	theme	conventional	1715:1726	arg1	formulation					1733:1743	conventional oral formulation	1715:1743	conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively)	1715:1840	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	0	107	theme	in	113:114	arg1	performance					121:131	in vivo performance	113:131	in vivo performance	113:131	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	7	108	theme	carbopol	1485:1492	arg1	7:3					1500:1502	7:3	1500:1502	7:3	1500:1502	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	108	theme	carbopol	1485:1492	arg1	974P					1494:1497	carbopol 974P	1485:1497	carbopol 974P (7:3)	1485:1503	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	109	dep	pattern	1314:1320	arg1	%					1328:1328	20-30%	1323:1328	20-30% at 2h	1323:1334	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	10	110	theme	hepatic	2100:2106	arg1	metabolism					2119:2128	hepatic first-pass metabolism	2100:2128	hepatic first-pass metabolism	2100:2128	The prepared provesicular buccoadhesive tablet of granisetron (F19) might help bypass hepatic first-pass metabolism and improve bioavailability of granisetron with the possibility of reducing reported daily dose (2mg) and reducing dosing frequency.
24793896	4	111	theme	reconstituted	775:787	arg1	niosomes					789:796	the reconstituted niosomes	771:796	the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration	771:906	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	6	112	theme	polymer	1159:1165	arg1	type					1167:1170	bioadhesive polymer type	1147:1170	bioadhesive polymer type	1147:1170	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	0	113	theme	performance	121:131	arg1	evaluation					69:78	in vitro evaluation	60:78	in vitro evaluation	60:78	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	113	theme	performance	121:131	arg1	investigation					96:108	preliminary investigation	84:108	preliminary investigation of in vivo performance	84:131	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	113	theme	performance	121:131	arg1	formulations					46:57	Provesicular granisetron hydrochloride buccal formulations	0:57	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.	0:132	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	8	114	theme	granisetron	1748:1758	arg1	formulation					1733:1743	conventional oral formulation	1715:1743	conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively)	1715:1840	In vivo pharmacokinetic study revealed higher bioavailability of buccal formulation relative to conventional oral formulation of granisetron (AUC0-∞ is 89.97 and 38.18 ng h/ml for buccal and oral formulation, respectively).
24793896	5	115	theme	encapsulation	1035:1047	arg1	efficiency					1049:1058	higher encapsulation efficiency	1028:1058	higher encapsulation efficiency	1028:1058	Span 80 proniosome-derived niosomes exhibited higher encapsulation efficiency and smaller particle size than those derived from span 20.
24793896	9	116	theme	buccal	1927:1932	arg1	application					1934:1944	buccal application	1927:1944	buccal application compared to conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h)	1927:2011	A significantly lower and delayed Cmax (12.09±4.47 ng/ml, at 8h) was observed after buccal application compared to conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h).
24793896	3	117	theme	buccal	440:445	arg1	delivery					452:459	buccal drug delivery	440:459	buccal drug delivery	440:459	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	0	118	theme	granisetron	13:23	arg1	evaluation					69:78	in vitro evaluation	60:78	in vitro evaluation	60:78	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	118	theme	granisetron	13:23	arg1	investigation					96:108	preliminary investigation	84:108	preliminary investigation of in vivo performance	84:131	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	118	theme	granisetron	13:23	arg1	formulations					46:57	Provesicular granisetron hydrochloride buccal formulations	0:57	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.	0:132	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	7	119	dep	precursors	1548:1557	arg1	precursors					1548:1557	the vesicular precursors span 80 and cholesterol (9:1)	1534:1587	the vesicular precursors span 80 and cholesterol (9:1)	1534:1587	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	119	dep	precursors	1548:1557	arg1	9:1					1584:1586	9:1	1584:1586	9:1	1584:1586	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	119	dep	precursors	1548:1557	arg1	span					1559:1562	span 80	1559:1565	span 80	1559:1565	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	119	dep	precursors	1548:1557	arg1	cholesterol					1571:1581	cholesterol	1571:1581	cholesterol (9:1)	1571:1587	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	5	120	theme	Span	982:985	arg1	niosomes					1009:1016	Span 80 proniosome-derived niosomes	982:1016	Span 80 proniosome-derived niosomes	982:1016	Span 80 proniosome-derived niosomes exhibited higher encapsulation efficiency and smaller particle size than those derived from span 20.
24793896	3	121	theme	carriers	427:434	arg1	advantage					401:409	the combined advantage	388:409	the combined advantage of provesicular carriers and buccal drug delivery	388:459	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	0	122	theme	buccal	39:44	arg1	evaluation					69:78	in vitro evaluation	60:78	in vitro evaluation	60:78	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	122	theme	buccal	39:44	arg1	investigation					96:108	preliminary investigation	84:108	preliminary investigation of in vivo performance	84:131	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	0	122	theme	buccal	39:44	arg1	formulations					46:57	Provesicular granisetron hydrochloride buccal formulations	0:57	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.	0:132	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	5	123	theme	proniosome-derived	990:1007	arg1	niosomes					1009:1016	Span 80 proniosome-derived niosomes	982:1016	Span 80 proniosome-derived niosomes	982:1016	Span 80 proniosome-derived niosomes exhibited higher encapsulation efficiency and smaller particle size than those derived from span 20.
24793896	0	124	dep	in	113:114	arg1	vivo					116:119	vivo	116:119	vivo	116:119	Provesicular granisetron hydrochloride buccal formulations: in vitro evaluation and preliminary investigation of in vivo performance.
24793896	9	125	theme	conventional	1958:1969	arg1	tablet					1976:1981	conventional oral tablet	1958:1981	conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h)	1958:2011	A significantly lower and delayed Cmax (12.09±4.47 ng/ml, at 8h) was observed after buccal application compared to conventional oral tablet (31.66±10.15 ng/ml, at 0.5 h).
24793896	7	126	theme	in	1297:1298	arg1	pattern					1314:1320	required in vitro release pattern	1288:1320	required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h)	1288:1367	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	3	127	dep	in	725:726	arg1	vivo					728:731	vivo	728:731	vivo	728:731	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	128	theme	drug	662:665	arg1	content					667:673	drug content	662:673	drug content	662:673	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	128	theme	drug	662:665	arg1	characteristics					635:649	suitable quality characteristics	618:649	suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior)	618:753	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	3	129	theme	delivery	452:459	arg1	advantage					401:409	the combined advantage	388:409	the combined advantage of provesicular carriers and buccal drug delivery	388:459	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	6	130	theme	in	1175:1176	arg1	release					1189:1195	in vitro drug release	1175:1195	in vitro drug release	1175:1195	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	3	131	theme	combined	392:399	arg1	advantage					401:409	the combined advantage	388:409	the combined advantage of provesicular carriers and buccal drug delivery	388:459	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
24793896	4	132	theme	surfactant	862:871	arg1	concentration					894:906	cholesterol concentration	882:906	cholesterol concentration	882:906	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	4	132	theme	surfactant	862:871	arg1	type					854:857	type	854:857	type of surfactant used	854:876	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	7	133	dep	%	1328:1328	arg1	%					1359:1359	80-95%	1354:1359	80-95% at 12h	1354:1366	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	7	133	dep	%	1328:1328	arg1	%					1342:1342	40-65%	1337:1342	40-65% at 6h	1337:1348	Based on achievement of required in vitro release pattern (20-30% at 2h, 40-65% at 6h and 80-95% at 12h), in vivo swelling behavior, and in vivo adhesion time (>14 h) granisetron formulation (F19, 1.4 mg) comprising HPMC:carbopol 974P (7:3) and maltodextrin coated with the vesicular precursors span 80 and cholesterol (9:1) was chosen for in vivo study.
24793896	6	134	theme	type	1167:1170	arg1	effect					1129:1134	the effect	1125:1134	the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets	1125:1244	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	4	135	theme	prepared	813:820	arg1	carriers					835:842	different prepared provesicular carriers	803:842	different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration	803:906	Composition of the reconstituted niosomes from different prepared provesicular carriers regarding type of surfactant used and cholesterol concentration significantly affected both entrapment efficiency (%EE) and vesicle size.
24793896	5	136	link	proniosome-derived	990:1007	arg1	niosomes					1009:1016	Span 80 proniosome-derived niosomes	982:1016	Span 80 proniosome-derived niosomes	982:1016	Span 80 proniosome-derived niosomes exhibited higher encapsulation efficiency and smaller particle size than those derived from span 20.
24793896	5	137	theme	particle	1072:1079	arg1	size					1081:1084	smaller particle size	1064:1084	smaller particle size	1064:1084	Span 80 proniosome-derived niosomes exhibited higher encapsulation efficiency and smaller particle size than those derived from span 20.
24793896	6	138	theme	bioadhesive	1147:1157	arg1	type					1167:1170	bioadhesive polymer type	1147:1170	bioadhesive polymer type	1147:1170	Also, the effect of %EE and bioadhesive polymer type on in vitro drug release and in vivo performance of buccoadhesive tablets was investigated.
24793896	2	139	theme	hepatic	354:360	arg1	metabolism					362:371	hepatic metabolism	354:371	hepatic metabolism	354:371	Granisetron suffers from reduced oral bioavailability (≈60%) due to hepatic metabolism.
24793896	3	140	dep	in	676:677	arg1	vitro					679:683	vitro	679:683	vitro	679:683	In this study the combined advantage of provesicular carriers and buccal drug delivery has been explored aiming to sustain effect and improve bioavailability of granisetron via development of granisetron provesicular buccoadhesive tablets with suitable quality characteristics (hardness, drug content, in vitro release pattern, exvivo bioadhesion and in vivo bioadhesion behavior).
27357127	12	0	theme	butyrate	2095:2102	arg1	concentrations					2104:2117	butyrate concentrations	2095:2117	butyrate concentrations	2095:2117	CONCLUSIONS We document the heterogeneous responses in butyrate concentrations upon RS supplementation and identify characteristic of the microbiota that appear to underlie this variation.
27357127	12	1	from	responses	2082:2090	arg1	concentrations					2104:2117	butyrate concentrations	2095:2117	butyrate concentrations	2095:2117	CONCLUSIONS We document the heterogeneous responses in butyrate concentrations upon RS supplementation and identify characteristic of the microbiota that appear to underlie this variation.
27357127	3	2	theme	starch-resistant	575:590	arg1	RS					600:601	RS	600:601	RS	600:601	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	3	2	theme	starch-resistant	575:590	arg1	starch					592:597	unmodified potato starch-resistant starch	557:597	unmodified potato starch-resistant starch (RS) type 2	557:609	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	11	3	dep	rectale	1960:1966	arg1	%					1988:1988	3 %	1986:1988	3 % in enhanced and low groups	1986:2015	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	11	3	dep	rectale	1960:1966	arg1	%					1972:1972	~6 %	1969:1972	~6 % in high	1969:1980	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	1	4	theme	dietary	121:127	arg1	fiber					129:133	dietary fiber	121:133	dietary fiber	121:133	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	4	5	theme	butyrate	683:690	arg1	concentration					692:704	butyrate concentration	683:704	butyrate concentration	683:704	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	3	6	theme	unmodified	557:566	arg1	RS					600:601	RS	600:601	RS	600:601	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	3	6	theme	unmodified	557:566	arg1	starch					592:597	unmodified potato starch-resistant starch	557:597	unmodified potato starch-resistant starch (RS) type 2	557:609	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	8	7	dep	15 mmol/kg	1363:1372	arg1	to					1360:1361	to	1360:1361	to	1360:1361	Fecal butyrate increased by 67 % in the enhanced group (from 9 to 15 mmol/kg), while it remained ≥11 mmol/kg in the high group and ≤8 mmol/kg in the low group.
27357127	7	8	dep	groups	1175:1180	arg1	high					1249:1252	high	1249:1252	high	1249:1252	Individuals could be categorized into three groups based upon butyrate concentrations before and during RS: enhanced, high, and low (n = 11, 3, and 6, respectively).
27357127	7	8	dep	groups	1175:1180	arg1	low					1259:1261	low	1259:1261	low	1259:1261	Individuals could be categorized into three groups based upon butyrate concentrations before and during RS: enhanced, high, and low (n = 11, 3, and 6, respectively).
27357127	1	9	theme	fiber	129:133	arg1	fermentation					105:116	The fermentation	101:116	The fermentation of dietary fiber to various organic acids	101:158	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	1	9	theme	fiber	129:133	arg1	function					176:183	a beneficial function	163:183	a beneficial function provided by the microbiota in the human large intestine	163:239	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	5	10	theme	butyrate	917:924	arg1	production					926:935	butyrate production	917:935	butyrate production	917:935	Random Forest and LEfSe analyses were used to associate responses in butyrate production to features of the microbiota.
27357127	6	11	theme	butyrate	1011:1018	arg1	concentrations					1020:1033	fecal butyrate concentrations	1005:1033	fecal butyrate concentrations	1005:1033	RESULTS RS supplementation increased fecal butyrate concentrations in this cohort from 8 to 12 mmol/kg wet feces, but responses varied widely between individuals.
27357127	11	12	dep	microbe	1940:1946	arg1	rectale					1960:1966	Eubacterium rectale	1948:1966	the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups)	1924:2016	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	11	13	from	group	1871:1875	arg1	microbiota					1833:1842	The microbiota	1829:1842	The microbiota of individuals in the high group	1829:1875	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	5	14	theme	microbiota	956:965	arg1	features					940:947	features	940:947	features of the microbiota	940:965	Random Forest and LEfSe analyses were used to associate responses in butyrate production to features of the microbiota.
27357127	6	15	theme	RS	976:977	arg1	supplementation					979:993	RESULTS RS supplementation	968:993	RESULTS RS supplementation	968:993	RESULTS RS supplementation increased fecal butyrate concentrations in this cohort from 8 to 12 mmol/kg wet feces, but responses varied widely between individuals.
27357127	2	16	theme	butyric	257:263	arg1	acid					265:268	butyric acid	257:268	butyric acid	257:268	In particular, butyric acid contributes to host health by facilitating maintenance of epithelial integrity, regulating inflammation, and influencing gene expression in colonocytes.
27357127	3	17	theme	potato	568:573	arg1	RS					600:601	RS	600:601	RS	600:601	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	3	17	theme	potato	568:573	arg1	starch					592:597	unmodified potato starch-resistant starch	557:597	unmodified potato starch-resistant starch (RS) type 2	557:609	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	1	18	theme	various	138:144	arg1	acids					154:158	various organic acids	138:158	various organic acids	138:158	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	11	19	from	individuals	1847:1857	arg1	group					1871:1875	the high group	1862:1875	the high group	1862:1875	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	8	20	from	≤8 mmol/kg	1428:1437	arg1	group					1418:1422	the high group	1409:1422	the high group	1409:1422	Fecal butyrate increased by 67 % in the enhanced group (from 9 to 15 mmol/kg), while it remained ≥11 mmol/kg in the high group and ≤8 mmol/kg in the low group.
27357127	8	20	from	≤8 mmol/kg	1428:1437	arg1	group					1450:1454	the low group	1442:1454	the low group	1442:1454	Fecal butyrate increased by 67 % in the enhanced group (from 9 to 15 mmol/kg), while it remained ≥11 mmol/kg in the high group and ≤8 mmol/kg in the low group.
27357127	9	21	theme	relative	1495:1502	arg1	abundance					1504:1512	the relative abundance	1491:1512	the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus	1491:1583	Microbiota analyses revealed that the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus bromii-increased from ~2 to 9 % in the enhanced and high groups, but remained at ~1.5 % in the low group.
27357127	1	22	dep	BACKGROUND	90:99	arg1	fermentation					105:116	The fermentation	101:116	The fermentation of dietary fiber to various organic acids	101:158	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	1	22	dep	BACKGROUND	90:99	arg1	function					176:183	a beneficial function	163:183	a beneficial function provided by the microbiota in the human large intestine	163:239	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	11	23	theme	microbe	1940:1946	arg1	abundance					1911:1919	an elevated abundance	1899:1919	an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study	1899:2037	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	4	24	theme	sequences	804:812	arg1	surveys					770:776	surveys	770:776	surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform	770:845	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	5	25	used	used	886:889	arg2	analyses					872:879	Random Forest and LEfSe analyses	848:879	Random Forest and LEfSe analyses	848:879	Random Forest and LEfSe analyses were used to associate responses in butyrate production to features of the microbiota.
27357127	9	26	theme	organisms-Bifidobacterium	1530:1554	arg1	adolescentis					1556:1567	RS-degrading organisms-Bifidobacterium adolescentis	1517:1567	RS-degrading organisms-Bifidobacterium adolescentis	1517:1567	Microbiota analyses revealed that the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus bromii-increased from ~2 to 9 % in the enhanced and high groups, but remained at ~1.5 % in the low group.
27357127	6	27	dep	12 mmol/kg	1060:1069	arg1	to					1057:1058	to	1057:1058	to	1057:1058	RESULTS RS supplementation increased fecal butyrate concentrations in this cohort from 8 to 12 mmol/kg wet feces, but responses varied widely between individuals.
27357127	11	28	from	microbiota	1833:1842	arg1	group					1871:1875	the high group	1862:1875	the high group	1862:1875	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	2	29	theme	gene	391:394	arg1	colonocytes					410:420	gene expression in colonocytes	391:420	gene expression in colonocytes	391:420	In particular, butyric acid contributes to host health by facilitating maintenance of epithelial integrity, regulating inflammation, and influencing gene expression in colonocytes.
27357127	0	30	theme	Variable	0:7	arg1	responses					9:17	Variable responses	0:17	Variable responses of human microbiomes to dietary supplementation with resistant starch	0:87	Variable responses of human microbiomes to dietary supplementation with resistant starch.
27357127	10	31	from	increase	1783:1790	arg1	butyrate					1801:1808	fecal butyrate	1795:1808	fecal butyrate	1795:1808	The lack of increase in RS-degrading bacteria in the low group may explain why there was no increase in fecal butyrate in response to RS.
27357127	7	32	dep	low	1259:1261	arg1	3					1272:1272	3	1272:1272	3	1272:1272	Individuals could be categorized into three groups based upon butyrate concentrations before and during RS: enhanced, high, and low (n = 11, 3, and 6, respectively).
27357127	7	32	dep	low	1259:1261	arg1	6					1279:1279	6	1279:1279	6	1279:1279	Individuals could be categorized into three groups based upon butyrate concentrations before and during RS: enhanced, high, and low (n = 11, 3, and 6, respectively).
27357127	7	32	dep	low	1259:1261	arg1	n = 11					1264:1269	n = 11	1264:1269	n = 11	1264:1269	Individuals could be categorized into three groups based upon butyrate concentrations before and during RS: enhanced, high, and low (n = 11, 3, and 6, respectively).
27357127	4	33	theme	rRNA-encoding	785:797	arg1	sequences					804:812	16S rRNA-encoding gene sequences	781:812	16S rRNA-encoding gene sequences from the Illumina MiSeq platform	781:845	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	5	34	theme	LEfSe	866:870	arg1	analyses					872:879	Random Forest and LEfSe analyses	848:879	Random Forest and LEfSe analyses	848:879	Random Forest and LEfSe analyses were used to associate responses in butyrate production to features of the microbiota.
27357127	10	35	theme	increase	1703:1710	arg1	lack					1695:1698	The lack	1691:1698	The lack of increase in RS-degrading bacteria in the low group	1691:1752	The lack of increase in RS-degrading bacteria in the low group may explain why there was no increase in fecal butyrate in response to RS.
27357127	3	36	dep	starch	549:554	arg1	RS					600:601	RS	600:601	RS	600:601	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	3	36	dep	starch	549:554	arg1	starch					592:597	unmodified potato starch-resistant starch	557:597	unmodified potato starch-resistant starch (RS) type 2	557:609	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	1	37	theme	organic	146:152	arg1	acids					154:158	various organic acids	138:158	various organic acids	138:158	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	11	38	theme	high	1866:1869	arg1	group					1871:1875	the high group	1862:1875	the high group	1862:1875	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	3	39	theme	butyrate	466:473	arg1	concentration					449:461	the concentration	445:461	the concentration of butyrate in 20 healthy young adults	445:500	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	0	40	theme	microbiomes	28:38	arg1	responses					9:17	Variable responses	0:17	Variable responses of human microbiomes to dietary supplementation with resistant starch	0:87	Variable responses of human microbiomes to dietary supplementation with resistant starch.
27357127	0	41	theme	dietary	43:49	arg1	supplementation					51:65	dietary supplementation	43:65	dietary supplementation with resistant starch	43:87	Variable responses of human microbiomes to dietary supplementation with resistant starch.
27357127	13	42	theme	gut	2359:2361	arg1	microbiomes					2363:2373	gut microbiomes	2359:2373	gut microbiomes	2359:2373	This study complements and extends other studies that call for personalized approaches to manage beneficial functions provided by gut microbiomes.
27357127	11	43	from	%	1972:1972	arg1	high					1977:1980	high	1977:1980	high	1977:1980	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	4	44	theme	MiSeq	832:836	arg1	platform					838:845	the Illumina MiSeq platform	819:845	the Illumina MiSeq platform	819:845	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	3	45	theme	young	489:493	arg1	adults					495:500	20 healthy young adults	478:500	20 healthy young adults	478:500	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	1	46	theme	human	219:223	arg1	intestine					231:239	the human large intestine	215:239	the human large intestine	215:239	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	10	47	dep	RS	1825:1826	arg1	response					1813:1820	response	1813:1820	response	1813:1820	The lack of increase in RS-degrading bacteria in the low group may explain why there was no increase in fecal butyrate in response to RS.
27357127	8	48	theme	high	1413:1416	arg1	group					1418:1422	the high group	1409:1422	the high group	1409:1422	Fecal butyrate increased by 67 % in the enhanced group (from 9 to 15 mmol/kg), while it remained ≥11 mmol/kg in the high group and ≤8 mmol/kg in the low group.
27357127	3	49	theme	resistant	539:547	arg1	starch					549:554	resistant starch	539:554	resistant starch (unmodified potato starch-resistant starch (RS) type 2)	539:610	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	13	50	theme	personalized	2292:2303	arg1	approaches					2305:2314	personalized approaches	2292:2314	personalized approaches	2292:2314	This study complements and extends other studies that call for personalized approaches to manage beneficial functions provided by gut microbiomes.
27357127	6	51	theme	RESULTS	968:974	arg1	supplementation					979:993	RESULTS RS supplementation	968:993	RESULTS RS supplementation	968:993	RESULTS RS supplementation increased fecal butyrate concentrations in this cohort from 8 to 12 mmol/kg wet feces, but responses varied widely between individuals.
27357127	9	52	theme	high	1637:1640	arg1	groups					1642:1647	high groups	1637:1647	high groups	1637:1647	Microbiota analyses revealed that the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus bromii-increased from ~2 to 9 % in the enhanced and high groups, but remained at ~1.5 % in the low group.
27357127	3	53	with	supplementation	518:532	arg1	starch					549:554	resistant starch	539:554	resistant starch (unmodified potato starch-resistant starch (RS) type 2)	539:610	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	8	54	from	15 mmol/kg	1363:1372	arg1	group					1346:1350	the enhanced group	1333:1350	the enhanced group (from 9 to 15 mmol/kg)	1333:1373	Fecal butyrate increased by 67 % in the enhanced group (from 9 to 15 mmol/kg), while it remained ≥11 mmol/kg in the high group and ≤8 mmol/kg in the low group.
27357127	2	55	theme	host	285:288	arg1	health					290:295	host health	285:295	host health	285:295	In particular, butyric acid contributes to host health by facilitating maintenance of epithelial integrity, regulating inflammation, and influencing gene expression in colonocytes.
27357127	11	56	dep	groups	2010:2015	arg1	low					2006:2008	low	2006:2008	low	2006:2008	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	11	56	dep	groups	2010:2015	arg1	enhanced					1993:2000	enhanced	1993:2000	enhanced	1993:2000	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	10	57	theme	RS-degrading	1715:1726	arg1	bacteria					1728:1735	RS-degrading bacteria	1715:1735	RS-degrading bacteria in the low group	1715:1752	The lack of increase in RS-degrading bacteria in the low group may explain why there was no increase in fecal butyrate in response to RS.
27357127	1	58	theme	beneficial	165:174	arg1	fermentation					105:116	The fermentation	101:116	The fermentation of dietary fiber to various organic acids	101:158	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	1	58	theme	beneficial	165:174	arg1	function					176:183	a beneficial function	163:183	a beneficial function provided by the microbiota in the human large intestine	163:239	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	10	59	from	lack	1695:1698	arg1	bacteria					1728:1735	RS-degrading bacteria	1715:1735	RS-degrading bacteria in the low group	1715:1752	The lack of increase in RS-degrading bacteria in the low group may explain why there was no increase in fecal butyrate in response to RS.
27357127	10	60	from	increase	1703:1710	arg1	bacteria					1728:1735	RS-degrading bacteria	1715:1735	RS-degrading bacteria in the low group	1715:1752	The lack of increase in RS-degrading bacteria in the low group may explain why there was no increase in fecal butyrate in response to RS.
27357127	9	61	dep	9 	1613:1614	arg1	to					1610:1611	to	1610:1611	to	1610:1611	Microbiota analyses revealed that the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus bromii-increased from ~2 to 9 % in the enhanced and high groups, but remained at ~1.5 % in the low group.
27357127	10	62	theme	low	1744:1746	arg1	group					1748:1752	the low group	1740:1752	the low group	1740:1752	The lack of increase in RS-degrading bacteria in the low group may explain why there was no increase in fecal butyrate in response to RS.
27357127	9	63	theme	low	1680:1682	arg1	group					1684:1688	the low group	1676:1688	the low group	1676:1688	Microbiota analyses revealed that the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus bromii-increased from ~2 to 9 % in the enhanced and high groups, but remained at ~1.5 % in the low group.
27357127	2	64	theme	integrity	339:347	arg1	maintenance					313:323	maintenance	313:323	maintenance of epithelial integrity	313:347	In particular, butyric acid contributes to host health by facilitating maintenance of epithelial integrity, regulating inflammation, and influencing gene expression in colonocytes.
27357127	11	65	theme	elevated	1902:1909	arg1	abundance					1911:1919	an elevated abundance	1899:1919	an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study	1899:2037	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	8	66	from	≥11 mmol/kg	1394:1404	arg1	group					1418:1422	the high group	1409:1422	the high group	1409:1422	Fecal butyrate increased by 67 % in the enhanced group (from 9 to 15 mmol/kg), while it remained ≥11 mmol/kg in the high group and ≤8 mmol/kg in the low group.
27357127	8	66	from	≥11 mmol/kg	1394:1404	arg1	group					1450:1454	the low group	1442:1454	the low group	1442:1454	Fecal butyrate increased by 67 % in the enhanced group (from 9 to 15 mmol/kg), while it remained ≥11 mmol/kg in the high group and ≤8 mmol/kg in the low group.
27357127	13	67	theme	other	2264:2268	arg1	studies					2270:2276	other studies	2264:2276	other studies that call for personalized approaches to manage beneficial functions provided by gut microbiomes	2264:2373	This study complements and extends other studies that call for personalized approaches to manage beneficial functions provided by gut microbiomes.
27357127	6	68	theme	fecal	1005:1009	arg1	concentrations					1020:1033	fecal butyrate concentrations	1005:1033	fecal butyrate concentrations	1005:1033	RESULTS RS supplementation increased fecal butyrate concentrations in this cohort from 8 to 12 mmol/kg wet feces, but responses varied widely between individuals.
27357127	11	69	theme	butyrogenic	1928:1938	arg1	microbe					1940:1946	the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups)	1924:2016	the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups)	1924:2016	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	9	70	theme	Microbiota	1457:1466	arg1	analyses					1468:1475	Microbiota analyses	1457:1475	Microbiota analyses	1457:1475	Microbiota analyses revealed that the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus bromii-increased from ~2 to 9 % in the enhanced and high groups, but remained at ~1.5 % in the low group.
27357127	8	71	theme	low	1446:1448	arg1	group					1450:1454	the low group	1442:1454	the low group	1442:1454	Fecal butyrate increased by 67 % in the enhanced group (from 9 to 15 mmol/kg), while it remained ≥11 mmol/kg in the high group and ≤8 mmol/kg in the low group.
27357127	8	72	theme	Fecal	1297:1301	arg1	butyrate					1303:1310	Fecal butyrate	1297:1310	Fecal butyrate	1297:1310	Fecal butyrate increased by 67 % in the enhanced group (from 9 to 15 mmol/kg), while it remained ≥11 mmol/kg in the high group and ≤8 mmol/kg in the low group.
27357127	10	73	theme	fecal	1795:1799	arg1	butyrate					1801:1808	fecal butyrate	1795:1808	fecal butyrate	1795:1808	The lack of increase in RS-degrading bacteria in the low group may explain why there was no increase in fecal butyrate in response to RS.
27357127	1	74	from	microbiota	201:210	arg1	intestine					231:239	the human large intestine	215:239	the human large intestine	215:239	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	12	75	theme	microbiota	2178:2187	arg1	characteristic					2156:2169	characteristic	2156:2169	characteristic of the microbiota that appear to underlie this variation	2156:2226	CONCLUSIONS We document the heterogeneous responses in butyrate concentrations upon RS supplementation and identify characteristic of the microbiota that appear to underlie this variation.
27357127	4	76	theme	microbiota	755:764	arg1	composition					736:746	composition	736:746	composition	736:746	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	4	76	theme	microbiota	755:764	arg1	chromatography					717:730	liquid chromatography	710:730	liquid chromatography	710:730	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	2	77	theme	epithelial	328:337	arg1	integrity					339:347	epithelial integrity	328:347	epithelial integrity	328:347	In particular, butyric acid contributes to host health by facilitating maintenance of epithelial integrity, regulating inflammation, and influencing gene expression in colonocytes.
27357127	0	78	theme	resistant	72:80	arg1	starch					82:87	resistant starch	72:87	resistant starch	72:87	Variable responses of human microbiomes to dietary supplementation with resistant starch.
27357127	4	79	dep	METHODS	613:619	arg1	collected					640:648	collected	640:648	were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform	635:845	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	4	80	from	platform	838:845	arg1	sequences					804:812	16S rRNA-encoding gene sequences	781:812	16S rRNA-encoding gene sequences from the Illumina MiSeq platform	781:845	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	4	80	from	platform	838:845	arg1	surveys					770:776	surveys	770:776	surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform	770:845	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	3	81	dep	starch	592:597	arg1	type					604:607	type 2	604:609	unmodified potato starch-resistant starch (RS) type 2	557:609	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	10	82	from	bacteria	1728:1735	arg1	lack					1695:1698	The lack	1691:1698	The lack of increase in RS-degrading bacteria in the low group	1691:1752	The lack of increase in RS-degrading bacteria in the low group may explain why there was no increase in fecal butyrate in response to RS.
27357127	10	82	from	bacteria	1728:1735	arg1	group					1748:1752	the low group	1740:1752	the low group	1740:1752	The lack of increase in RS-degrading bacteria in the low group may explain why there was no increase in fecal butyrate in response to RS.
27357127	6	83	theme	wet	1071:1073	arg1	feces					1075:1079	8 to 12 mmol/kg wet feces	1055:1079	8 to 12 mmol/kg wet feces	1055:1079	RESULTS RS supplementation increased fecal butyrate concentrations in this cohort from 8 to 12 mmol/kg wet feces, but responses varied widely between individuals.
27357127	4	84	theme	liquid	710:715	arg1	chromatography					717:730	liquid chromatography	710:730	liquid chromatography	710:730	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	5	85	from	responses	904:912	arg1	production					926:935	butyrate production	917:935	butyrate production	917:935	Random Forest and LEfSe analyses were used to associate responses in butyrate production to features of the microbiota.
27357127	11	86	from	%	1988:1988	arg1	groups					2010:2015	enhanced and low groups	1993:2015	enhanced and low groups	1993:2015	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	12	87	theme	heterogeneous	2068:2080	arg1	responses					2082:2090	the heterogeneous responses	2064:2090	the heterogeneous responses in butyrate concentrations upon RS supplementation	2064:2141	CONCLUSIONS We document the heterogeneous responses in butyrate concentrations upon RS supplementation and identify characteristic of the microbiota that appear to underlie this variation.
27357127	9	88	theme	RS-degrading	1517:1528	arg1	adolescentis					1556:1567	RS-degrading organisms-Bifidobacterium adolescentis	1517:1567	RS-degrading organisms-Bifidobacterium adolescentis	1517:1567	Microbiota analyses revealed that the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus bromii-increased from ~2 to 9 % in the enhanced and high groups, but remained at ~1.5 % in the low group.
27357127	6	89	from	feces	1075:1079	arg1	cohort					1043:1048	this cohort	1038:1048	this cohort from 8 to 12 mmol/kg wet feces	1038:1079	RESULTS RS supplementation increased fecal butyrate concentrations in this cohort from 8 to 12 mmol/kg wet feces, but responses varied widely between individuals.
27357127	4	90	theme	gene	799:802	arg1	sequences					804:812	16S rRNA-encoding gene sequences	781:812	16S rRNA-encoding gene sequences from the Illumina MiSeq platform	781:845	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	9	91	theme	adolescentis	1556:1567	arg1	abundance					1504:1512	the relative abundance	1491:1512	the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus	1491:1583	Microbiota analyses revealed that the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus bromii-increased from ~2 to 9 % in the enhanced and high groups, but remained at ~1.5 % in the low group.
27357127	2	92	theme	expression in	396:408	arg1	colonocytes					410:420	gene expression in colonocytes	391:420	gene expression in colonocytes	391:420	In particular, butyric acid contributes to host health by facilitating maintenance of epithelial integrity, regulating inflammation, and influencing gene expression in colonocytes.
27357127	5	93	theme	Random	848:853	arg1	analyses					872:879	Random Forest and LEfSe analyses	848:879	Random Forest and LEfSe analyses	848:879	Random Forest and LEfSe analyses were used to associate responses in butyrate production to features of the microbiota.
27357127	9	94	theme	Ruminococcus	1572:1583	arg1	abundance					1504:1512	the relative abundance	1491:1512	the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus	1491:1583	Microbiota analyses revealed that the relative abundance of RS-degrading organisms-Bifidobacterium adolescentis or Ruminococcus bromii-increased from ~2 to 9 % in the enhanced and high groups, but remained at ~1.5 % in the low group.
27357127	11	95	theme	individuals	1847:1857	arg1	microbiota					1833:1842	The microbiota	1829:1842	The microbiota of individuals in the high group	1829:1875	The microbiota of individuals in the high group were characterized by an elevated abundance of the butyrogenic microbe Eubacterium rectale (~6 % in high vs. 3 % in enhanced and low groups) throughout the study.
27357127	0	96	theme	human	22:26	arg1	microbiomes					28:38	human microbiomes	22:38	human microbiomes	22:38	Variable responses of human microbiomes to dietary supplementation with resistant starch.
27357127	5	97	theme	Forest	855:860	arg1	analyses					872:879	Random Forest and LEfSe analyses	848:879	Random Forest and LEfSe analyses	848:879	Random Forest and LEfSe analyses were used to associate responses in butyrate production to features of the microbiota.
27357127	4	98	from	surveys	770:776	arg1	platform					838:845	the Illumina MiSeq platform	819:845	the Illumina MiSeq platform	819:845	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	4	99	theme	16S	781:783	arg1	sequences					804:812	16S rRNA-encoding gene sequences	781:812	16S rRNA-encoding gene sequences from the Illumina MiSeq platform	781:845	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	3	100	theme	healthy	481:487	arg1	adults					495:500	20 healthy young adults	478:500	20 healthy young adults	478:500	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	7	101	theme	butyrate	1193:1200	arg1	concentrations					1202:1215	butyrate concentrations	1193:1215	butyrate concentrations before and during RS	1193:1236	Individuals could be categorized into three groups based upon butyrate concentrations before and during RS: enhanced, high, and low (n = 11, 3, and 6, respectively).
27357127	13	102	theme	beneficial	2326:2335	arg1	functions					2337:2345	beneficial functions	2326:2345	beneficial functions provided by gut microbiomes	2326:2373	This study complements and extends other studies that call for personalized approaches to manage beneficial functions provided by gut microbiomes.
27357127	12	103	theme	RS	2124:2125	arg1	supplementation					2127:2141	RS supplementation	2124:2141	RS supplementation	2124:2141	CONCLUSIONS We document the heterogeneous responses in butyrate concentrations upon RS supplementation and identify characteristic of the microbiota that appear to underlie this variation.
27357127	3	104	theme	dietary	510:516	arg1	supplementation					518:532	dietary supplementation	510:532	dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2)	510:610	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
27357127	12	105	dep	CONCLUSIONS	2040:2050	arg1	document					2055:2062	document	2055:2062	document the heterogeneous responses in butyrate concentrations upon RS supplementation	2055:2141	CONCLUSIONS We document the heterogeneous responses in butyrate concentrations upon RS supplementation and identify characteristic of the microbiota that appear to underlie this variation.
27357127	12	105	dep	CONCLUSIONS	2040:2050	arg1	identify					2147:2154	identify	2147:2154	identify characteristic of the microbiota that appear to underlie this variation	2147:2226	CONCLUSIONS We document the heterogeneous responses in butyrate concentrations upon RS supplementation and identify characteristic of the microbiota that appear to underlie this variation.
27357127	4	106	theme	Fecal	621:625	arg1	samples					627:633	Fecal samples	621:633	Fecal samples	621:633	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	4	107	theme	Illumina	823:830	arg1	platform					838:845	the Illumina MiSeq platform	819:845	the Illumina MiSeq platform	819:845	METHODS Fecal samples were collected from individuals to characterize butyrate concentration via liquid chromatography and composition of the microbiota via surveys of 16S rRNA-encoding gene sequences from the Illumina MiSeq platform.
27357127	0	108	with	supplementation	51:65	arg1	starch					82:87	resistant starch	72:87	resistant starch	72:87	Variable responses of human microbiomes to dietary supplementation with resistant starch.
27357127	8	109	theme	enhanced	1337:1344	arg1	group					1346:1350	the enhanced group	1333:1350	the enhanced group (from 9 to 15 mmol/kg)	1333:1373	Fecal butyrate increased by 67 % in the enhanced group (from 9 to 15 mmol/kg), while it remained ≥11 mmol/kg in the high group and ≤8 mmol/kg in the low group.
27357127	1	110	theme	large	225:229	arg1	intestine					231:239	the human large intestine	215:239	the human large intestine	215:239	BACKGROUND The fermentation of dietary fiber to various organic acids is a beneficial function provided by the microbiota in the human large intestine.
27357127	3	111	from	concentration	449:461	arg1	adults					495:500	20 healthy young adults	478:500	20 healthy young adults	478:500	We sought to increase the concentration of butyrate in 20 healthy young adults through dietary supplementation with resistant starch (unmodified potato starch-resistant starch (RS) type 2).
26701794	9	0	from	SA	1374:1375	arg1	cerebellum					1384:1393	the cerebellum	1380:1393	the cerebellum of pigs	1380:1401	Similarly, ganglioside-bound SA in the cerebellum of pigs fed 4 g 3'-sialyllactose/L increased by 10% in comparison with control pigs.
26701794	6	1	theme	digesta	1032:1038	arg1	composition					1002:1012	Microbial composition	992:1012	Microbial composition of the intestinal digesta	992:1038	Microbial composition of the intestinal digesta was analyzed with the use of 16S ribosomal DNA Illumina sequencing.
26701794	2	2	theme	ganglioside-bound	351:367	arg1	SA					369:370	ganglioside-bound SA	351:370	ganglioside-bound SA	351:370	Breastfed infants have increased accumulation of ganglioside-bound SA compared with formula-fed infants.
26701794	3	3	theme	developing	537:546	arg1	piglets					557:563	developing neonatal piglets	537:563	developing neonatal piglets	537:563	OBJECTIVE This study aimed to determine whether different isomers of sialyllactose enrich brain SA and modulate the microbiome of developing neonatal piglets.
26701794	10	4	theme	Significant	1480:1490	arg1	differences					1527:1537	Significant (P < 0.05, Adonis Test) microbiome differences	1480:1537	Significant (P < 0.05, Adonis Test) microbiome differences	1480:1537	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	11	5	theme	P	1906:1906	arg1	<					1908:1908	P < 0.05	1906:1913	P < 0.05	1906:1913	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	9	6	with	comparison	1450:1459	arg1	pigs					1474:1477	control pigs	1466:1477	control pigs	1466:1477	Similarly, ganglioside-bound SA in the cerebellum of pigs fed 4 g 3'-sialyllactose/L increased by 10% in comparison with control pigs.
26701794	0	7	theme	Dietary	0:6	arg1	Isomers					8:14	Dietary Isomers	0:14	Dietary Isomers of Sialyllactose	0:31	Dietary Isomers of Sialyllactose Increase Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum and Modulate the Colonic Microbiota of Formula-Fed Piglets.
26701794	6	8	theme	Illumina	1087:1094	arg1	sequencing					1096:1105	ribosomal DNA Illumina sequencing	1073:1105	ribosomal DNA Illumina sequencing	1073:1105	Microbial composition of the intestinal digesta was analyzed with the use of 16S ribosomal DNA Illumina sequencing.
26701794	2	9	theme	Breastfed	302:310	arg1	infants					312:318	Breastfed infants	302:318	Breastfed infants	302:318	Breastfed infants have increased accumulation of ganglioside-bound SA compared with formula-fed infants.
26701794	14	10	theme	SA	2525:2526	arg1	microbiota					2580:2589	beneficial microbiota	2569:2589	beneficial microbiota	2569:2589	We propose 2 potential routes by which sialyllactose may positively affect the neonate: serving as a source of SA for neurologic development and promoting beneficial microbiota.
26701794	14	10	theme	SA	2525:2526	arg1	source					2515:2520	a source	2513:2520	a source of SA for neurologic development	2513:2553	We propose 2 potential routes by which sialyllactose may positively affect the neonate: serving as a source of SA for neurologic development and promoting beneficial microbiota.
26701794	6	11	theme	ribosomal	1073:1081	arg1	sequencing					1096:1105	ribosomal DNA Illumina sequencing	1073:1105	ribosomal DNA Illumina sequencing	1073:1105	Microbial composition of the intestinal digesta was analyzed with the use of 16S ribosomal DNA Illumina sequencing.
26701794	8	12	theme	Ganglioside-bound	1196:1212	arg1	SA					1214:1215	Ganglioside-bound SA	1196:1215	Ganglioside-bound SA in the corpus callosum of pigs	1196:1246	Ganglioside-bound SA in the corpus callosum of pigs fed 2 g 3'-sialyllactose or 6'-sialyllactose/L increased by 15% in comparison with control pigs.
26701794	8	13	with	comparison	1315:1324	arg1	pigs					1339:1342	control pigs	1331:1342	control pigs	1331:1342	Ganglioside-bound SA in the corpus callosum of pigs fed 2 g 3'-sialyllactose or 6'-sialyllactose/L increased by 15% in comparison with control pigs.
26701794	13	14	theme	gut-associated	2370:2383	arg1	microbiota					2385:2394	gut-associated microbiota	2370:2394	gut-associated microbiota in neonatal pigs	2370:2411	CONCLUSIONS Supplementation of formula with 3'- or 6'-sialyllactose can enrich ganglioside SA in the brain and modulate gut-associated microbiota in neonatal pigs.
26701794	14	15	theme	neurologic	2532:2541	arg1	development					2543:2553	neurologic development	2532:2553	neurologic development	2532:2553	We propose 2 potential routes by which sialyllactose may positively affect the neonate: serving as a source of SA for neurologic development and promoting beneficial microbiota.
26701794	1	16	theme	human	213:217	arg1	Sialyllactose					190:202	BACKGROUND Sialyllactose	179:202	BACKGROUND Sialyllactose	179:202	BACKGROUND Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule.
26701794	1	16	theme	human	213:217	arg1	oligosaccharide					224:238	a key human milk oligosaccharide	207:238	a key human milk oligosaccharide	207:238	BACKGROUND Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule.
26701794	11	17	dep	test	1900:1903	arg1	<					1908:1908	P < 0.05	1906:1913	P < 0.05	1906:1913	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	11	17	dep	test	1900:1903	arg1	DeSeq2					1916:1921	DeSeq2	1916:1921	DeSeq2	1916:1921	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	10	18	dep	Significant	1480:1490	arg1	Test					1510:1513	Adonis Test	1503:1513	Adonis Test	1503:1513	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	10	18	dep	Significant	1480:1490	arg1	P					1493:1493	P < 0.05	1493:1500	P < 0.05	1493:1500	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	8	19	dep	3'-sialyllactose	1256:1271	arg1	/L					1292:1293	/L	1292:1293	/L	1292:1293	Ganglioside-bound SA in the corpus callosum of pigs fed 2 g 3'-sialyllactose or 6'-sialyllactose/L increased by 15% in comparison with control pigs.
26701794	11	20	theme	control	1954:1960	arg1	diet					1962:1965	the control diet	1950:1965	the control diet	1950:1965	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	0	21	from	Concentrations	66:79	arg1	Cerebellum					108:117	Cerebellum	108:117	Cerebellum	108:117	Dietary Isomers of Sialyllactose Increase Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum and Modulate the Colonic Microbiota of Formula-Fed Piglets.
26701794	0	21	from	Concentrations	66:79	arg1	Callosum					95:102	the Corpus Callosum	84:102	the Corpus Callosum	84:102	Dietary Isomers of Sialyllactose Increase Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum and Modulate the Colonic Microbiota of Formula-Fed Piglets.
26701794	8	22	theme	corpus	1224:1229	arg1	callosum					1231:1238	the corpus callosum	1220:1238	the corpus callosum of pigs	1220:1246	Ganglioside-bound SA in the corpus callosum of pigs fed 2 g 3'-sialyllactose or 6'-sialyllactose/L increased by 15% in comparison with control pigs.
26701794	10	23	theme	Adonis	1503:1508	arg1	Test					1510:1513	Adonis Test	1503:1513	Adonis Test	1503:1513	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	10	23	theme	Adonis	1503:1508	arg1	P					1493:1493	P < 0.05	1493:1500	P < 0.05	1493:1500	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	5	24	theme	periodic	957:964	arg1	reaction					982:989	a modified periodic acid-resorcinol reaction	946:989	a modified periodic acid-resorcinol reaction	946:989	SA was determined by using a modified periodic acid-resorcinol reaction.
26701794	10	25	theme	/L	1644:1645	arg1	formulas					1647:1654	4-g 6'-sialyllactose/L formulas	1624:1654	4-g 6'-sialyllactose/L formulas	1624:1654	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	9	26	theme	g	1409:1409	arg1	/L					1427:1428	4 g 3'-sialyllactose/L	1407:1428	4 g 3'-sialyllactose/L	1407:1428	Similarly, ganglioside-bound SA in the cerebellum of pigs fed 4 g 3'-sialyllactose/L increased by 10% in comparison with control pigs.
26701794	0	27	theme	Piglets	170:176	arg1	Microbiota					144:153	the Colonic Microbiota	132:153	the Colonic Microbiota of Formula-Fed Piglets	132:176	Dietary Isomers of Sialyllactose Increase Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum and Modulate the Colonic Microbiota of Formula-Fed Piglets.
26701794	12	28	theme	6'-sialyllactose-fed	2203:2222	arg1	piglets					2224:2230	6'-sialyllactose-fed piglets	2203:2230	6'-sialyllactose-fed piglets	2203:2230	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	4	29	theme	6'-sialyllactose	670:685	arg1	/L					686:687	2 or 4 g 6'-sialyllactose/L	661:687	2 or 4 g 6'-sialyllactose/L	661:687	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	2	30	contain	have	320:323	arg2	accumulation					335:346	increased accumulation	325:346	increased accumulation of ganglioside-bound SA	325:370	Breastfed infants have increased accumulation of ganglioside-bound SA compared with formula-fed infants.
26701794	2	30	contain	have	320:323	arg1	infants					312:318	Breastfed infants	302:318	Breastfed infants	302:318	Breastfed infants have increased accumulation of ganglioside-bound SA compared with formula-fed infants.
26701794	4	31	dep	=	748:748	arg1	g					716:716	2 g	714:716	2 g	714:716	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	4	31	dep	=	748:748	arg1	g					639:639	2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L	632:710	2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L	632:710	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	4	31	dep	=	748:748	arg1	control					623:629	control	623:629	control	623:629	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	13	32	from	SA	2341:2342	arg1	brain					2351:2355	the brain	2347:2355	the brain	2347:2355	CONCLUSIONS Supplementation of formula with 3'- or 6'-sialyllactose can enrich ganglioside SA in the brain and modulate gut-associated microbiota in neonatal pigs.
26701794	4	33	dep	cerebrum	855:862	arg1	regions					910:916	regions	910:916	regions	910:916	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	11	34	dep	aerofaciens	1751:1761	arg1	Firmicutes					1837:1846	phylum Firmicutes	1830:1846	phylum Firmicutes	1830:1846	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	3	35	theme	different	455:463	arg1	isomers					465:471	different isomers	455:471	different isomers of sialyllactose	455:488	OBJECTIVE This study aimed to determine whether different isomers of sialyllactose enrich brain SA and modulate the microbiome of developing neonatal piglets.
26701794	9	36	theme	control	1466:1472	arg1	pigs					1474:1477	control pigs	1466:1477	control pigs	1466:1477	Similarly, ganglioside-bound SA in the cerebellum of pigs fed 4 g 3'-sialyllactose/L increased by 10% in comparison with control pigs.
26701794	7	37	theme	Dietary	1116:1122	arg1	sialyllactose					1124:1136	RESULTS Dietary sialyllactose	1108:1136	RESULTS Dietary sialyllactose	1108:1136	RESULTS Dietary sialyllactose did not affect feed intake, growth, or fecal consistency.
26701794	13	38	theme	neonatal	2399:2406	arg1	pigs					2408:2411	neonatal pigs	2399:2411	neonatal pigs	2399:2411	CONCLUSIONS Supplementation of formula with 3'- or 6'-sialyllactose can enrich ganglioside SA in the brain and modulate gut-associated microbiota in neonatal pigs.
26701794	6	39	theme	Microbial	992:1000	arg1	composition					1002:1012	Microbial composition	992:1012	Microbial composition of the intestinal digesta	992:1038	Microbial composition of the intestinal digesta was analyzed with the use of 16S ribosomal DNA Illumina sequencing.
26701794	12	40	theme	family	2088:2093	arg1	Lachnospiraceae					2095:2109	family Lachnospiraceae	2088:2109	family Lachnospiraceae	2088:2109	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	10	41	theme	distal	1573:1578	arg1	colons					1580:1585	the proximal and distal colons	1556:1585	the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas	1556:1654	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	11	42	theme	Wald	1895:1898	arg1	Prevotella					1860:1869	genus Prevotella	1854:1869	genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2)	1854:1922	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	11	42	theme	Wald	1895:1898	arg1	test					1900:1903	Wald test	1895:1903	Wald test	1895:1903	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	10	43	theme	proximal	1560:1567	arg1	colons					1580:1585	the proximal and distal colons	1556:1585	the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas	1556:1654	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	9	44	theme	ganglioside-bound	1356:1372	arg1	SA					1374:1375	ganglioside-bound SA	1356:1375	ganglioside-bound SA in the cerebellum of pigs	1356:1401	Similarly, ganglioside-bound SA in the cerebellum of pigs fed 4 g 3'-sialyllactose/L increased by 10% in comparison with control pigs.
26701794	14	45	theme	potential	2427:2435	arg1	routes					2437:2442	2 potential routes	2425:2442	2 potential routes by which sialyllactose may positively affect the neonate	2425:2499	We propose 2 potential routes by which sialyllactose may positively affect the neonate: serving as a source of SA for neurologic development and promoting beneficial microbiota.
26701794	8	46	theme	control	1331:1337	arg1	pigs					1339:1342	control pigs	1331:1342	control pigs	1331:1342	Ganglioside-bound SA in the corpus callosum of pigs fed 2 g 3'-sialyllactose or 6'-sialyllactose/L increased by 15% in comparison with control pigs.
26701794	0	47	theme	Sialic	54:59	arg1	Concentrations					66:79	Ganglioside Sialic Acid Concentrations	42:79	Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum	42:117	Dietary Isomers of Sialyllactose Increase Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum and Modulate the Colonic Microbiota of Formula-Fed Piglets.
26701794	5	48	theme	modified	948:955	arg1	reaction					982:989	a modified periodic acid-resorcinol reaction	946:989	a modified periodic acid-resorcinol reaction	946:989	SA was determined by using a modified periodic acid-resorcinol reaction.
26701794	12	49	theme	phylum	2035:2040	arg1	Proteobacteria					2042:2055	phylum Proteobacteria	2035:2055	phylum Proteobacteria	2035:2055	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	12	49	theme	phylum	2035:2040	arg1	Enterococcaceae					2018:2032	Enterococcaceae	2018:2032	Enterococcaceae (phylum Proteobacteria)	2018:2056	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	2	50	theme	increased	325:333	arg1	accumulation					335:346	increased accumulation	325:346	increased accumulation of ganglioside-bound SA	325:370	Breastfed infants have increased accumulation of ganglioside-bound SA compared with formula-fed infants.
26701794	10	51	theme	6'-sialyllactose	1628:1643	arg1	formulas					1647:1654	4-g 6'-sialyllactose/L formulas	1624:1654	4-g 6'-sialyllactose/L formulas	1624:1654	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	4	52	theme	g	668:668	arg1	/L					686:687	2 or 4 g 6'-sialyllactose/L	661:687	2 or 4 g 6'-sialyllactose/L	661:687	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	10	53	theme	4-g	1624:1626	arg1	formulas					1647:1654	4-g 6'-sialyllactose/L formulas	1624:1654	4-g 6'-sialyllactose/L formulas	1624:1654	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	12	54	dep	families	1986:1993	arg1	Proteobacteria					2042:2055	phylum Proteobacteria	2035:2055	phylum Proteobacteria	2035:2055	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	12	54	dep	families	1986:1993	arg1	Enterobacteriaceae					1995:2012	Enterobacteriaceae	1995:2012	Enterobacteriaceae	1995:2012	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	12	54	dep	families	1986:1993	arg1	families					1986:1993	families	1986:1993	families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria)	1986:2056	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	12	54	dep	families	1986:1993	arg1	Enterococcaceae					2018:2032	Enterococcaceae	2018:2032	Enterococcaceae (phylum Proteobacteria)	2018:2056	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	7	55	theme	feed	1153:1156	arg1	intake					1158:1163	feed intake	1153:1163	feed intake	1153:1163	RESULTS Dietary sialyllactose did not affect feed intake, growth, or fecal consistency.
26701794	2	56	theme	SA	369:370	arg1	accumulation					335:346	increased accumulation	325:346	increased accumulation of ganglioside-bound SA	325:370	Breastfed infants have increased accumulation of ganglioside-bound SA compared with formula-fed infants.
26701794	6	57	theme	intestinal	1021:1030	arg1	digesta					1032:1038	the intestinal digesta	1017:1038	the intestinal digesta	1017:1038	Microbial composition of the intestinal digesta was analyzed with the use of 16S ribosomal DNA Illumina sequencing.
26701794	4	58	theme	d.	778:779	arg1	Pigs					781:784	21 d. Pigs	775:784	21 d. Pigs	775:784	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	4	59	theme	left	807:810	arg1	hemisphere					812:821	the left hemisphere	803:821	the left hemisphere of the brain	803:834	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	10	60	located	observed	1544:1551	arg2	differences					1527:1537	Significant (P < 0.05, Adonis Test) microbiome differences	1480:1537	Significant (P < 0.05, Adonis Test) microbiome differences	1480:1537	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	10	60	located	observed	1544:1551	arg1	colons					1580:1585	the proximal and distal colons	1556:1585	the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas	1556:1654	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	3	61	theme	piglets	557:563	arg1	microbiome					523:532	the microbiome	519:532	the microbiome of developing neonatal piglets	519:563	OBJECTIVE This study aimed to determine whether different isomers of sialyllactose enrich brain SA and modulate the microbiome of developing neonatal piglets.
26701794	1	62	theme	BACKGROUND	179:188	arg1	Sialyllactose					190:202	BACKGROUND Sialyllactose	179:202	BACKGROUND Sialyllactose	179:202	BACKGROUND Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule.
26701794	1	62	theme	BACKGROUND	179:188	arg1	oligosaccharide					224:238	a key human milk oligosaccharide	207:238	a key human milk oligosaccharide	207:238	BACKGROUND Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule.
26701794	13	63	with	Supplementation	2262:2276	arg1	6'-sialyllactose					2301:2316	6'-sialyllactose	2301:2316	6'-sialyllactose	2301:2316	CONCLUSIONS Supplementation of formula with 3'- or 6'-sialyllactose can enrich ganglioside SA in the brain and modulate gut-associated microbiota in neonatal pigs.
26701794	13	63	with	Supplementation	2262:2276	arg1	3'-					2294:2296	3'-	2294:2296	3'-	2294:2296	CONCLUSIONS Supplementation of formula with 3'- or 6'-sialyllactose can enrich ganglioside SA in the brain and modulate gut-associated microbiota in neonatal pigs.
26701794	11	64	from	increase	1691:1698	arg1	taxa					1713:1716	bacterial taxa	1703:1716	bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet	1703:1965	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	11	65	dep	Prevotella	1860:1869	arg1	Bacteroidetes					1879:1891	phylum Bacteroidetes	1872:1891	phylum Bacteroidetes	1872:1891	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	6	66	theme	DNA	1083:1085	arg1	sequencing					1096:1105	ribosomal DNA Illumina sequencing	1073:1105	ribosomal DNA Illumina sequencing	1073:1105	Microbial composition of the intestinal digesta was analyzed with the use of 16S ribosomal DNA Illumina sequencing.
26701794	10	67	theme	<	1495:1495	arg1	Test					1510:1513	Adonis Test	1503:1513	Adonis Test	1503:1513	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	10	67	theme	<	1495:1495	arg1	P					1493:1493	P < 0.05	1493:1500	P < 0.05	1493:1500	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	11	68	theme	bacterial	1703:1711	arg1	taxa					1713:1716	bacterial taxa	1703:1716	bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet	1703:1965	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	13	69	theme	formula	2281:2287	arg1	Supplementation					2262:2276	CONCLUSIONS Supplementation	2250:2276	CONCLUSIONS Supplementation of formula with 3'- or 6'-sialyllactose	2250:2316	CONCLUSIONS Supplementation of formula with 3'- or 6'-sialyllactose can enrich ganglioside SA in the brain and modulate gut-associated microbiota in neonatal pigs.
26701794	1	70	theme	key	209:211	arg1	Sialyllactose					190:202	BACKGROUND Sialyllactose	179:202	BACKGROUND Sialyllactose	179:202	BACKGROUND Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule.
26701794	1	70	theme	key	209:211	arg1	oligosaccharide					224:238	a key human milk oligosaccharide	207:238	a key human milk oligosaccharide	207:238	BACKGROUND Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule.
26701794	10	71	theme	microbiome	1516:1525	arg1	differences					1527:1537	Significant (P < 0.05, Adonis Test) microbiome differences	1480:1537	Significant (P < 0.05, Adonis Test) microbiome differences	1480:1537	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	11	72	theme	phylum	1764:1769	arg1	aerofaciens					1751:1761	Collinsella aerofaciens	1739:1761	Collinsella aerofaciens (phylum Actinobacteria)	1739:1785	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	11	72	theme	phylum	1764:1769	arg1	Actinobacteria					1771:1784	phylum Actinobacteria	1764:1784	phylum Actinobacteria	1764:1784	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	9	73	theme	pigs	1398:1401	arg1	cerebellum					1384:1393	the cerebellum	1380:1393	the cerebellum of pigs	1380:1401	Similarly, ganglioside-bound SA in the cerebellum of pigs fed 4 g 3'-sialyllactose/L increased by 10% in comparison with control pigs.
26701794	4	74	theme	3'-sialyllactose	641:656	arg1	g					639:639	2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L	632:710	2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L	632:710	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	0	75	theme	Formula-Fed	158:168	arg1	Piglets					170:176	Formula-Fed Piglets	158:176	Formula-Fed Piglets	158:176	Dietary Isomers of Sialyllactose Increase Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum and Modulate the Colonic Microbiota of Formula-Fed Piglets.
26701794	5	76	theme	acid-resorcinol	966:980	arg1	reaction					982:989	a modified periodic acid-resorcinol reaction	946:989	a modified periodic acid-resorcinol reaction	946:989	SA was determined by using a modified periodic acid-resorcinol reaction.
26701794	9	77	theme	3'-sialyllactose	1411:1426	arg1	/L					1427:1428	4 g 3'-sialyllactose/L	1407:1428	4 g 3'-sialyllactose/L	1407:1428	Similarly, ganglioside-bound SA in the cerebellum of pigs fed 4 g 3'-sialyllactose/L increased by 10% in comparison with control pigs.
26701794	4	78	theme	brain	830:834	arg1	hemisphere					812:821	the left hemisphere	803:821	the left hemisphere of the brain	803:834	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	4	79	theme	g	695:695	arg1	polydextrose/L					697:710	2 g polydextrose/L	693:710	2 g polydextrose/L	693:710	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	8	80	from	SA	1214:1215	arg1	callosum					1231:1238	the corpus callosum	1220:1238	the corpus callosum of pigs	1220:1246	Ganglioside-bound SA in the corpus callosum of pigs fed 2 g 3'-sialyllactose or 6'-sialyllactose/L increased by 15% in comparison with control pigs.
26701794	8	81	theme	pigs	1243:1246	arg1	callosum					1231:1238	the corpus callosum	1220:1238	the corpus callosum of pigs	1220:1246	Ganglioside-bound SA in the corpus callosum of pigs fed 2 g 3'-sialyllactose or 6'-sialyllactose/L increased by 15% in comparison with control pigs.
26701794	0	82	theme	Corpus	88:93	arg1	Callosum					95:102	the Corpus Callosum	84:102	the Corpus Callosum	84:102	Dietary Isomers of Sialyllactose Increase Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum and Modulate the Colonic Microbiota of Formula-Fed Piglets.
26701794	4	83	dep	METHODS	566:572	arg1	fed					757:759	fed	757:759	fed 3 times/d for 21 d. Pigs	757:784	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	4	83	dep	METHODS	566:572	arg1	allocated					601:609	allocated	601:609	were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9)	587:751	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	13	84	from	microbiota	2385:2394	arg1	pigs					2408:2411	neonatal pigs	2399:2411	neonatal pigs	2399:2411	CONCLUSIONS Supplementation of formula with 3'- or 6'-sialyllactose can enrich ganglioside SA in the brain and modulate gut-associated microbiota in neonatal pigs.
26701794	12	85	theme	phylum	2138:2143	arg1	Firmicutes					2145:2154	phylum Firmicutes	2138:2154	phylum Firmicutes	2138:2154	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	12	85	theme	phylum	2138:2143	arg1	Lactobacillales					2121:2135	order Lactobacillales	2115:2135	order Lactobacillales (phylum Firmicutes)	2115:2155	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	3	86	dep	OBJECTIVE	407:415	arg1	aimed					428:432	aimed	428:432	aimed to determine whether different isomers of sialyllactose enrich brain SA and modulate the microbiome of developing neonatal piglets	428:563	OBJECTIVE This study aimed to determine whether different isomers of sialyllactose enrich brain SA and modulate the microbiome of developing neonatal piglets.
26701794	14	87	theme	beneficial	2569:2578	arg1	microbiota					2580:2589	beneficial microbiota	2569:2589	beneficial microbiota	2569:2589	We propose 2 potential routes by which sialyllactose may positively affect the neonate: serving as a source of SA for neurologic development and promoting beneficial microbiota.
26701794	14	87	theme	beneficial	2569:2578	arg1	source					2515:2520	a source	2513:2520	a source of SA for neurologic development	2513:2553	We propose 2 potential routes by which sialyllactose may positively affect the neonate: serving as a source of SA for neurologic development and promoting beneficial microbiota.
26701794	13	88	theme	ganglioside	2329:2339	arg1	SA					2341:2342	ganglioside SA	2329:2342	ganglioside SA in the brain	2329:2355	CONCLUSIONS Supplementation of formula with 3'- or 6'-sialyllactose can enrich ganglioside SA in the brain and modulate gut-associated microbiota in neonatal pigs.
26701794	4	89	theme	corpus	877:882	arg1	callosum					884:891	corpus callosum	877:891	corpus callosum	877:891	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	12	90	theme	order	2115:2119	arg1	Firmicutes					2145:2154	phylum Firmicutes	2138:2154	phylum Firmicutes	2138:2154	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	12	90	theme	order	2115:2119	arg1	Lactobacillales					2121:2135	order Lactobacillales	2115:2135	order Lactobacillales (phylum Firmicutes)	2115:2155	Taxa belonging to families Enterobacteriaceae and Enterococcaceae (phylum Proteobacteria), as well as taxa belonging to family Lachnospiraceae and order Lactobacillales (phylum Firmicutes), were 2.3- and 4-fold lower, respectively, in 6'-sialyllactose-fed piglets than in controls.
26701794	7	91	theme	RESULTS	1108:1114	arg1	sialyllactose					1124:1136	RESULTS Dietary sialyllactose	1108:1136	RESULTS Dietary sialyllactose	1108:1136	RESULTS Dietary sialyllactose did not affect feed intake, growth, or fecal consistency.
26701794	11	92	dep	species	1731:1737	arg1	aerofaciens					1751:1761	Collinsella aerofaciens	1739:1761	Collinsella aerofaciens (phylum Actinobacteria)	1739:1785	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	11	92	dep	species	1731:1737	arg1	Ruminococcus					1795:1806	genera Ruminococcus	1788:1806	genera Ruminococcus	1788:1806	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	11	92	dep	species	1731:1737	arg1	Faecalibacterium					1812:1827	Faecalibacterium	1812:1827	Faecalibacterium	1812:1827	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	11	92	dep	species	1731:1737	arg1	Actinobacteria					1771:1784	phylum Actinobacteria	1764:1784	phylum Actinobacteria	1764:1784	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	11	92	dep	species	1731:1737	arg1	Prevotella					1860:1869	genus Prevotella	1854:1869	genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2)	1854:1922	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	11	92	dep	species	1731:1737	arg1	test					1900:1903	Wald test	1895:1903	Wald test	1895:1903	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	2	93	theme	formula-fed	386:396	arg1	infants					398:404	formula-fed infants	386:404	formula-fed infants	386:404	Breastfed infants have increased accumulation of ganglioside-bound SA compared with formula-fed infants.
26701794	0	94	theme	Sialyllactose	19:31	arg1	Isomers					8:14	Dietary Isomers	0:14	Dietary Isomers of Sialyllactose	0:31	Dietary Isomers of Sialyllactose Increase Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum and Modulate the Colonic Microbiota of Formula-Fed Piglets.
26701794	3	95	theme	brain	497:501	arg1	SA					503:504	brain SA	497:504	brain SA	497:504	OBJECTIVE This study aimed to determine whether different isomers of sialyllactose enrich brain SA and modulate the microbiome of developing neonatal piglets.
26701794	0	96	theme	Ganglioside	42:52	arg1	Concentrations					66:79	Ganglioside Sialic Acid Concentrations	42:79	Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum	42:117	Dietary Isomers of Sialyllactose Increase Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum and Modulate the Colonic Microbiota of Formula-Fed Piglets.
26701794	1	97	theme	lactose	284:290	arg1	molecule					292:299	a lactose molecule	282:299	a lactose molecule	282:299	BACKGROUND Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule.
26701794	1	98	theme	milk	219:222	arg1	Sialyllactose					190:202	BACKGROUND Sialyllactose	179:202	BACKGROUND Sialyllactose	179:202	BACKGROUND Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule.
26701794	1	98	theme	milk	219:222	arg1	oligosaccharide					224:238	a key human milk oligosaccharide	207:238	a key human milk oligosaccharide	207:238	BACKGROUND Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule.
26701794	13	99	theme	CONCLUSIONS	2250:2260	arg1	Supplementation					2262:2276	CONCLUSIONS Supplementation	2250:2276	CONCLUSIONS Supplementation of formula with 3'- or 6'-sialyllactose	2250:2316	CONCLUSIONS Supplementation of formula with 3'- or 6'-sialyllactose can enrich ganglioside SA in the brain and modulate gut-associated microbiota in neonatal pigs.
26701794	3	100	theme	sialyllactose	476:488	arg1	isomers					465:471	different isomers	455:471	different isomers of sialyllactose	455:488	OBJECTIVE This study aimed to determine whether different isomers of sialyllactose enrich brain SA and modulate the microbiome of developing neonatal piglets.
26701794	0	101	theme	Acid	61:64	arg1	Concentrations					66:79	Ganglioside Sialic Acid Concentrations	42:79	Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum	42:117	Dietary Isomers of Sialyllactose Increase Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum and Modulate the Colonic Microbiota of Formula-Fed Piglets.
26701794	4	102	dep	g	639:639	arg1	/L					686:687	2 or 4 g 6'-sialyllactose/L	661:687	2 or 4 g 6'-sialyllactose/L	661:687	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	4	102	dep	g	639:639	arg1	/L					657:658	/L	657:658	/L	657:658	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	4	102	dep	g	639:639	arg1	galacto-oligosaccharides/L					718:743	galacto-oligosaccharides/L	718:743	galacto-oligosaccharides/L	718:743	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	4	102	dep	g	639:639	arg1	polydextrose/L					697:710	2 g polydextrose/L	693:710	2 g polydextrose/L	693:710	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	0	103	theme	Colonic	136:142	arg1	Microbiota					144:153	the Colonic Microbiota	132:153	the Colonic Microbiota of Formula-Fed Piglets	132:176	Dietary Isomers of Sialyllactose Increase Ganglioside Sialic Acid Concentrations in the Corpus Callosum and Cerebellum and Modulate the Colonic Microbiota of Formula-Fed Piglets.
26701794	4	104	theme	Day-old	574:580	arg1	pigs					582:585	Day-old pigs	574:585	Day-old pigs	574:585	METHODS Day-old pigs were randomly allocated to 6 diets (control, 2 or 4 g 3'-sialyllactose/L, 2 or 4 g 6'-sialyllactose/L, or 2 g polydextrose/L + 2 g galacto-oligosaccharides/L; n = 9) and fed 3 times/d for 21 d. Pigs were killed, and the left hemisphere of the brain was dissected into cerebrum, cerebellum, corpus callosum, and hippocampus regions.
26701794	10	105	theme	piglets	1590:1596	arg1	colons					1580:1585	the proximal and distal colons	1556:1585	the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas	1556:1654	Significant (P < 0.05, Adonis Test) microbiome differences were observed in the proximal and distal colons of piglets fed control compared with 4-g 6'-sialyllactose/L formulas.
26701794	9	106	dep	fed	1403:1405	arg1	increased					1430:1438	increased	1430:1438	fed 4 g 3'-sialyllactose/L increased by 10% in comparison with control pigs	1403:1477	Similarly, ganglioside-bound SA in the cerebellum of pigs fed 4 g 3'-sialyllactose/L increased by 10% in comparison with control pigs.
26701794	11	107	theme	genera	1788:1793	arg1	Ruminococcus					1795:1806	genera Ruminococcus	1788:1806	genera Ruminococcus	1788:1806	Differences were attributed to an increase in bacterial taxa belonging to species Collinsella aerofaciens (phylum Actinobacteria), genera Ruminococcus and Faecalibacterium (phylum Firmicutes), and genus Prevotella (phylum Bacteroidetes) (Wald test, P < 0.05, DeSeq2) compared with piglets fed the control diet.
26701794	8	108	dep	fed	1248:1250	arg1	increased					1295:1303	increased	1295:1303	fed 2 g 3'-sialyllactose or 6'-sialyllactose/L increased by 15% in comparison with control pigs	1248:1342	Ganglioside-bound SA in the corpus callosum of pigs fed 2 g 3'-sialyllactose or 6'-sialyllactose/L increased by 15% in comparison with control pigs.
26701794	7	109	theme	fecal	1177:1181	arg1	consistency					1183:1193	fecal consistency	1177:1193	fecal consistency	1177:1193	RESULTS Dietary sialyllactose did not affect feed intake, growth, or fecal consistency.
26701794	3	110	theme	neonatal	548:555	arg1	piglets					557:563	developing neonatal piglets	537:563	developing neonatal piglets	537:563	OBJECTIVE This study aimed to determine whether different isomers of sialyllactose enrich brain SA and modulate the microbiome of developing neonatal piglets.
26701794	1	111	theme	sialic	256:261	arg1	SA					269:270	SA	269:270	SA	269:270	BACKGROUND Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule.
26701794	1	111	theme	sialic	256:261	arg1	acid					263:266	sialic acid	256:266	sialic acid (SA) bound to a lactose molecule	256:299	BACKGROUND Sialyllactose is a key human milk oligosaccharide and consists of sialic acid (SA) bound to a lactose molecule.
27865958	5	0	theme	mechanical	795:804	arg1	similar					836:842	similar	836:842	similar	836:842	The mechanical strengths and porosities were similar to that of human spongy bone.
27865958	5	0	theme	mechanical	795:804	arg1	strengths					806:814	The mechanical strengths	791:814	The mechanical strengths	791:814	The mechanical strengths and porosities were similar to that of human spongy bone.
27865958	1	1	theme	bone-like	339:347	arg1	nanocomposites					349:362	bone-like nanocomposites	339:362	bone-like nanocomposites for bone tissue engineering application	339:402	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	8	2	with	compatibility	1416:1428	arg1	pH					1441:1442	human pH	1435:1442	human pH	1435:1442	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	8	2	with	compatibility	1416:1428	arg1	blood					1448:1452	blood	1448:1452	blood	1448:1452	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	8	3	theme	CS-PEG	1241:1246	arg1	composite					1259:1267	CS-PEG (55:30wt%) composite	1241:1267	CS-PEG (55:30wt%) composite	1241:1267	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	8	4	theme	HA-ZrO2	1203:1209	arg1	Addition					1185:1192	Addition	1185:1192	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite	1185:1267	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	9	5	theme	tissue	1537:1542	arg1	engineering					1544:1554	bone tissue engineering	1532:1554	bone tissue engineering	1532:1554	Thus, this nanocomposite could serve as a potential candidate to be used for bone tissue engineering.
27865958	4	6	theme	water	606:610	arg1	capacity					623:630	Enhanced water absorption capacity	597:630	Enhanced water absorption capacity	597:630	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	0	7	theme	biomedical	103:112	arg1	application					114:124	biomedical application	103:124	biomedical application	103:124	Development of bone-like zirconium oxide nanoceramic modified chitosan based porous nanocomposites for biomedical application.
27865958	1	8	theme	bone	368:371	arg1	engineering					380:390	bone tissue engineering	368:390	bone tissue engineering application	368:402	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	7	9	theme	alkalinity	1004:1013	arg1	values					1024:1029	low alkalinity pH (7.4) values	1000:1029	low alkalinity pH (7.4) values	1000:1029	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	7	9	theme	alkalinity	1004:1013	arg1	7.4					1019:1021	7.4	1019:1021	7.4	1019:1021	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	1	10	theme	ethylene	276:283	arg1	poly					271:274	poly	271:274	poly(ethylene glycol)	271:291	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	10	theme	ethylene	276:283	arg1	glycol					285:290	ethylene glycol	276:290	ethylene glycol	276:290	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	8	11	theme	having	1212:1217	arg1	HA-ZrO2					1203:1209	15wt% HA-ZrO2	1197:1209	15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs)	1197:1234	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	8	11	theme	having	1212:1217	arg1	NPs					1231:1233	having 0.3wt% ZrO2 NPs	1212:1233	having 0.3wt% ZrO2 NPs	1212:1233	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	1	12	theme	tissue	373:378	arg1	engineering					380:390	bone tissue engineering	368:390	bone tissue engineering application	368:402	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	7	13	theme	pH	1015:1016	arg1	values					1024:1029	low alkalinity pH (7.4) values	1000:1029	low alkalinity pH (7.4) values	1000:1029	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	7	13	theme	pH	1015:1016	arg1	7.4					1019:1021	7.4	1019:1021	7.4	1019:1021	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	8	14	theme	water	1386:1390	arg1	capacity					1403:1410	suitable water absorption capacity	1377:1410	suitable water absorption capacity	1377:1410	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	8	15	theme	ZrO2	1226:1229	arg1	HA-ZrO2					1203:1209	15wt% HA-ZrO2	1197:1209	15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs)	1197:1234	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	8	15	theme	ZrO2	1226:1229	arg1	NPs					1231:1233	having 0.3wt% ZrO2 NPs	1212:1233	having 0.3wt% ZrO2 NPs	1212:1233	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	3	16	dep	10μm	591:594	arg1	to					588:589	to	588:589	to	588:589	SEM images and porosity measurements revealed highly porous structure having pore size of less than 1μm to 10μm.
27865958	4	17	theme	Enhanced	597:604	arg1	capacity					623:630	Enhanced water absorption capacity	597:630	Enhanced water absorption capacity	597:630	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	1	18	theme	engineering	380:390	arg1	application					392:402	bone tissue engineering application	368:402	bone tissue engineering application	368:402	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	8	19	theme	suitable	1377:1384	arg1	capacity					1403:1410	suitable water absorption capacity	1377:1410	suitable water absorption capacity	1377:1410	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	9	20	theme	bone	1532:1535	arg1	engineering					1544:1554	bone tissue engineering	1532:1554	bone tissue engineering	1532:1554	Thus, this nanocomposite could serve as a potential candidate to be used for bone tissue engineering.
27865958	3	21	theme	pore	561:564	arg1	size					566:569	pore size	561:569	pore size of less than 1μm to 10μm	561:594	SEM images and porosity measurements revealed highly porous structure having pore size of less than 1μm to 10μm.
27865958	7	22	theme	human	1053:1057	arg1	plasma					1059:1064	human plasma	1053:1064	human plasma	1053:1064	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	8	23	theme	%	1224:1224	arg1	HA-ZrO2					1203:1209	15wt% HA-ZrO2	1197:1209	15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs)	1197:1234	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	8	23	theme	%	1224:1224	arg1	NPs					1231:1233	having 0.3wt% ZrO2 NPs	1212:1233	having 0.3wt% ZrO2 NPs	1212:1233	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	7	24	theme	MG-63	1125:1129	arg1	cells					1131:1135	osteoblastic MG-63 cells	1112:1135	osteoblastic MG-63 cells	1112:1135	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	7	25	theme	low	1000:1002	arg1	values					1024:1029	low alkalinity pH (7.4) values	1000:1029	low alkalinity pH (7.4) values	1000:1029	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	7	25	theme	low	1000:1002	arg1	7.4					1019:1021	7.4	1019:1021	7.4	1019:1021	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	6	26	theme	bacterial	943:951	arg1	strains					953:959	gram-negative and gram-positive bacterial strains	911:959	gram-negative and gram-positive bacterial strains	911:959	Strong antimicrobial effects against gram-negative and gram-positive bacterial strains were also observed.
27865958	4	27	theme	CS-PEG-HA	703:711	arg1	composite					713:721	previously reported CS-PEG-HA composite	683:721	previously reported CS-PEG-HA composite	683:721	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	8	28	theme	0.3wt	1219:1223	arg1	HA-ZrO2					1203:1209	15wt% HA-ZrO2	1197:1209	15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs)	1197:1234	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	8	28	theme	0.3wt	1219:1223	arg1	NPs					1231:1233	having 0.3wt% ZrO2 NPs	1212:1233	having 0.3wt% ZrO2 NPs	1212:1233	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	4	29	theme	NPs	760:762	arg1	%					750:750	0.1-0.3wt%	741:750	0.1-0.3wt% of ZrO2 NPs into these nanocomposites	741:788	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	4	29	theme	NPs	760:762	arg1	NPs					760:762	ZrO2 NPs	755:762	ZrO2 NPs into these nanocomposites	755:788	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	8	30	theme	15wt	1197:1200	arg1	HA-ZrO2					1203:1209	15wt% HA-ZrO2	1197:1209	15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs)	1197:1234	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	8	30	theme	15wt	1197:1200	arg1	NPs					1231:1233	having 0.3wt% ZrO2 NPs	1212:1233	having 0.3wt% ZrO2 NPs	1212:1233	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	4	31	theme	reported	694:701	arg1	composite					713:721	previously reported CS-PEG-HA composite	683:721	previously reported CS-PEG-HA composite	683:721	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	0	32	theme	zirconium	25:33	arg1	chitosan					62:69	bone-like zirconium oxide nanoceramic modified chitosan	15:69	bone-like zirconium oxide nanoceramic modified chitosan	15:69	Development of bone-like zirconium oxide nanoceramic modified chitosan based porous nanocomposites for biomedical application.
27865958	3	33	theme	less	574:577	arg1	size					566:569	pore size	561:569	pore size of less than 1μm to 10μm	561:594	SEM images and porosity measurements revealed highly porous structure having pore size of less than 1μm to 10μm.
27865958	1	34	theme	first	200:204	arg1	time					206:209	the first time	196:209	the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application	196:402	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	0	35	theme	bone-like	15:23	arg1	chitosan					62:69	bone-like zirconium oxide nanoceramic modified chitosan	15:69	bone-like zirconium oxide nanoceramic modified chitosan	15:69	Development of bone-like zirconium oxide nanoceramic modified chitosan based porous nanocomposites for biomedical application.
27865958	7	36	theme	osteoblastic	1112:1123	arg1	cells					1131:1135	osteoblastic MG-63 cells	1112:1135	osteoblastic MG-63 cells	1112:1135	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	6	37	theme	gram-negative	911:923	arg1	strains					953:959	gram-negative and gram-positive bacterial strains	911:959	gram-negative and gram-positive bacterial strains	911:959	Strong antimicrobial effects against gram-negative and gram-positive bacterial strains were also observed.
27865958	3	38	theme	SEM	484:486	arg1	images					488:493	SEM images	484:493	SEM images	484:493	SEM images and porosity measurements revealed highly porous structure having pore size of less than 1μm to 10μm.
27865958	0	39	theme	nanoceramic	41:51	arg1	chitosan					62:69	bone-like zirconium oxide nanoceramic modified chitosan	15:69	bone-like zirconium oxide nanoceramic modified chitosan	15:69	Development of bone-like zirconium oxide nanoceramic modified chitosan based porous nanocomposites for biomedical application.
27865958	4	40	theme	0.1-0.3wt	741:749	arg1	%					750:750	0.1-0.3wt%	741:750	0.1-0.3wt% of ZrO2 NPs into these nanocomposites	741:788	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	4	40	theme	0.1-0.3wt	741:749	arg1	NPs					760:762	ZrO2 NPs	755:762	ZrO2 NPs into these nanocomposites	755:788	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	9	41	theme	potential	1497:1505	arg1	nanocomposite					1466:1478	this nanocomposite	1461:1478	this nanocomposite	1461:1478	Thus, this nanocomposite could serve as a potential candidate to be used for bone tissue engineering.
27865958	9	41	theme	potential	1497:1505	arg1	candidate					1507:1515	a potential candidate	1495:1515	a potential candidate to be used for bone tissue engineering	1495:1554	Thus, this nanocomposite could serve as a potential candidate to be used for bone tissue engineering.
27865958	8	42	theme	human	1435:1439	arg1	pH					1441:1442	human pH	1435:1442	human pH	1435:1442	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	7	43	with	hemocompatibility	1067:1083	arg1	cells					1131:1135	osteoblastic MG-63 cells	1112:1135	osteoblastic MG-63 cells	1112:1135	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	8	44	theme	mechanical	1290:1299	arg1	strength					1301:1308	mechanical strength	1290:1308	mechanical strength	1290:1308	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	0	45	theme	oxide	35:39	arg1	chitosan					62:69	bone-like zirconium oxide nanoceramic modified chitosan	15:69	bone-like zirconium oxide nanoceramic modified chitosan	15:69	Development of bone-like zirconium oxide nanoceramic modified chitosan based porous nanocomposites for biomedical application.
27865958	1	46	contain	containing	250:259	arg2	glycol					285:290	ethylene glycol	276:290	ethylene glycol	276:290	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	46	contain	containing	250:259	arg2	poly					271:274	poly	271:274	poly(ethylene glycol)	271:291	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	46	contain	containing	250:259	arg2	nano-hydroxypatite					297:314	nano-hydroxypatite	297:314	nano-hydroxypatite (CS-PEG-HA)	297:326	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	46	contain	containing	250:259	arg1	composites					239:248	organic-inorganic hybrid composites	214:248	organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA)	214:326	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	46	contain	containing	250:259	arg2	CS-PEG-HA					317:325	CS-PEG-HA	317:325	CS-PEG-HA	317:325	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	46	contain	containing	250:259	arg2	chitosan					261:268	chitosan	261:268	chitosan	261:268	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	47	theme	organic-inorganic	214:230	arg1	composites					239:248	organic-inorganic hybrid composites	214:248	organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA)	214:326	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	0	48	theme	chitosan	62:69	arg1	Development					0:10	Development	0:10	Development of bone-like zirconium oxide nanoceramic modified chitosan	0:69	Development of bone-like zirconium oxide nanoceramic modified chitosan based porous nanocomposites for biomedical application.
27865958	3	49	contain	having	554:559	arg2	size					566:569	pore size	561:569	pore size of less than 1μm to 10μm	561:594	SEM images and porosity measurements revealed highly porous structure having pore size of less than 1μm to 10μm.
27865958	3	49	contain	having	554:559	arg1	structure					544:552	highly porous structure	530:552	highly porous structure having pore size of less than 1μm to 10μm	530:594	SEM images and porosity measurements revealed highly porous structure having pore size of less than 1μm to 10μm.
27865958	6	50	theme	antimicrobial	881:893	arg1	effects					895:901	Strong antimicrobial effects	874:901	Strong antimicrobial effects against gram-negative and gram-positive bacterial strains	874:959	Strong antimicrobial effects against gram-negative and gram-positive bacterial strains were also observed.
27865958	6	51	theme	gram-positive	929:941	arg1	strains					953:959	gram-negative and gram-positive bacterial strains	911:959	gram-negative and gram-positive bacterial strains	911:959	Strong antimicrobial effects against gram-negative and gram-positive bacterial strains were also observed.
27865958	3	52	theme	porosity	499:506	arg1	measurements					508:519	porosity measurements	499:519	porosity measurements	499:519	SEM images and porosity measurements revealed highly porous structure having pore size of less than 1μm to 10μm.
27865958	0	53	theme	modified	53:60	arg1	chitosan					62:69	bone-like zirconium oxide nanoceramic modified chitosan	15:69	bone-like zirconium oxide nanoceramic modified chitosan	15:69	Development of bone-like zirconium oxide nanoceramic modified chitosan based porous nanocomposites for biomedical application.
27865958	6	54	theme	Strong	874:879	arg1	effects					895:901	Strong antimicrobial effects	874:901	Strong antimicrobial effects against gram-negative and gram-positive bacterial strains	874:959	Strong antimicrobial effects against gram-negative and gram-positive bacterial strains were also observed.
27865958	1	55	theme	zirconium	133:141	arg1	nanoparticles					149:161	zirconium oxide nanoparticles	133:161	zirconium oxide nanoparticles (ZrO2 NPs)	133:172	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	55	theme	zirconium	133:141	arg1	NPs					169:171	ZrO2 NPs	164:171	ZrO2 NPs	164:171	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	7	56	theme	similar	1032:1038	arg1	values					1024:1029	low alkalinity pH (7.4) values	1000:1029	low alkalinity pH (7.4) values	1000:1029	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	7	56	theme	similar	1032:1038	arg1	7.4					1019:1021	7.4	1019:1021	7.4	1019:1021	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	4	57	theme	%	750:750	arg1	addition					729:736	addition	729:736	addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites	729:788	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	8	58	theme	absorption	1392:1401	arg1	capacity					1403:1410	suitable water absorption capacity	1377:1410	suitable water absorption capacity	1377:1410	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	1	59	from	time	206:209	arg1	composites					239:248	organic-inorganic hybrid composites	214:248	organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA)	214:326	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	60	theme	oxide	143:147	arg1	nanoparticles					149:161	zirconium oxide nanoparticles	133:161	zirconium oxide nanoparticles (ZrO2 NPs)	133:172	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	60	theme	oxide	143:147	arg1	NPs					169:171	ZrO2 NPs	164:171	ZrO2 NPs	164:171	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	61	theme	hybrid	232:237	arg1	composites					239:248	organic-inorganic hybrid composites	214:248	organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA)	214:326	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	7	62	theme	plasma	1059:1064	arg1	pH					1047:1048	the pH	1043:1048	the pH of human plasma	1043:1064	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	8	63	theme	antimicrobial	1321:1333	arg1	property					1335:1342	antimicrobial property	1321:1342	antimicrobial property	1321:1342	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	4	64	theme	ZrO2	755:758	arg1	NPs					760:762	ZrO2 NPs	755:762	ZrO2 NPs into these nanocomposites	755:788	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	0	65	theme	porous	77:82	arg1	nanocomposites					84:97	porous nanocomposites	77:97	porous nanocomposites for biomedical application	77:124	Development of bone-like zirconium oxide nanoceramic modified chitosan based porous nanocomposites for biomedical application.
27865958	8	66	theme	greatest	1281:1288	arg1	strength					1301:1308	mechanical strength	1290:1308	mechanical strength	1290:1308	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	5	67	theme	spongy	861:866	arg1	bone					868:871	human spongy bone	855:871	human spongy bone	855:871	The mechanical strengths and porosities were similar to that of human spongy bone.
27865958	3	68	theme	porous	537:542	arg1	structure					544:552	highly porous structure	530:552	highly porous structure having pore size of less than 1μm to 10μm	530:594	SEM images and porosity measurements revealed highly porous structure having pore size of less than 1μm to 10μm.
27865958	7	69	with	cytocompatibility	1089:1105	arg1	cells					1131:1135	osteoblastic MG-63 cells	1112:1135	osteoblastic MG-63 cells	1112:1135	Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites.
27865958	4	70	theme	mechanical	636:645	arg1	strengths					647:655	mechanical strengths	636:655	mechanical strengths	636:655	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	8	71	with	capacity	1403:1410	arg1	pH					1441:1442	human pH	1435:1442	human pH	1435:1442	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	8	71	with	capacity	1403:1410	arg1	blood					1448:1452	blood	1448:1452	blood	1448:1452	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	5	72	theme	human	855:859	arg1	bone					868:871	human spongy bone	855:871	human spongy bone	855:871	The mechanical strengths and porosities were similar to that of human spongy bone.
27865958	4	73	theme	absorption	612:621	arg1	capacity					623:630	Enhanced water absorption capacity	597:630	Enhanced water absorption capacity	597:630	Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites.
27865958	1	74	theme	ZrO2	164:167	arg1	nanoparticles					149:161	zirconium oxide nanoparticles	133:161	zirconium oxide nanoparticles (ZrO2 NPs)	133:172	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	1	74	theme	ZrO2	164:167	arg1	NPs					169:171	ZrO2 NPs	164:171	ZrO2 NPs	164:171	Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application.
27865958	8	75	theme	%	1201:1201	arg1	HA-ZrO2					1203:1209	15wt% HA-ZrO2	1197:1209	15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs)	1197:1234	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
27865958	8	75	theme	%	1201:1201	arg1	NPs					1231:1233	having 0.3wt% ZrO2 NPs	1212:1233	having 0.3wt% ZrO2 NPs	1212:1233	Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood.
28714596	3	0	theme	±	759:759	arg1	µm					763:764	58 ± 1 µm	756:764	58 ± 1 µm	756:764	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	3	0	theme	±	759:759	arg1	distribution					742:753	homogeneous particle size distribution	716:753	homogeneous particle size distribution (58 ± 1 µm)	716:765	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	5	1	theme	oral	1526:1529	arg1	composite					1501:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	5	1	theme	oral	1526:1529	arg1	system					1545:1550	a promising oral drug delivery system	1514:1550	a promising oral drug delivery system for drugs with poor oral bioavailability	1514:1591	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	2	2	theme	drug	658:661	arg1	absorption					663:672	drug absorption	658:672	drug absorption	658:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	3	3	theme	pH-responsive	772:784	arg1	behavior					798:805	pH-responsive dissolution behavior	772:805	pH-responsive dissolution behavior	772:805	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	5	4	theme	delivery	1536:1543	arg1	composite					1501:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	5	4	theme	delivery	1536:1543	arg1	system					1545:1550	a promising oral drug delivery system	1514:1550	a promising oral drug delivery system for drugs with poor oral bioavailability	1514:1591	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	4	5	mod	modification	1011:1022	arg3	surface					1003:1009	The surface modification	999:1022	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid)	999:1087	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	4	5	mod	modification	1011:1022	arg1	HNT					1027:1029	HNT	1027:1029	HNT	1027:1029	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	4	6	theme	mouse	1179:1183	arg1	intestines					1191:1200	the mouse small intestines	1175:1200	the mouse small intestines	1175:1200	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	1	7	theme	delivery	196:203	arg1	system					205:210	An advanced oral drug delivery system	174:210	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability	174:276	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability is strongly desirable.
28714596	1	7	theme	delivery	196:203	arg1	desirable					290:298	desirable	290:298	desirable	290:298	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability is strongly desirable.
28714596	3	8	theme	pH	810:811	arg1	>					813:813	pH > 6	810:815	pH > 6	810:815	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	4	9	theme	Caco-2/HT29-MTX	1149:1163	arg1	cells					1165:1169	the intestinal Caco-2/HT29-MTX cells	1134:1169	the intestinal Caco-2/HT29-MTX cells	1134:1169	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	0	10	theme	Site-Specific	140:152	arg1	Delivery					164:171	Site-Specific Oral Drug Delivery	140:171	Site-Specific Oral Drug Delivery	140:171	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	2	11	theme	nano-in-micro	327:339	arg1	composite					352:360	a multifunctional nano-in-micro structured composite	309:360	a multifunctional nano-in-micro structured composite	309:360	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	0	12	theme	Drug	159:162	arg1	Delivery					164:171	Site-Specific Oral Drug Delivery	140:171	Site-Specific Oral Drug Delivery	140:171	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	3	13	theme	premature	839:847	arg1	release					849:855	the premature release	835:855	the premature release of curcumin	835:867	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	3	14	theme	spherical	699:707	arg1	shape					709:713	spherical shape	699:713	spherical shape	699:713	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	5	15	with	drugs	1556:1560	arg1	bioavailability					1577:1591	poor oral bioavailability	1567:1591	poor oral bioavailability	1567:1591	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	3	16	theme	pH	882:883	arg1	conditions					885:894	simulated pH conditions	872:894	simulated pH conditions of the stomach	872:909	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	4	17	theme	HNT	1027:1029	arg1	modification					1011:1022	The surface modification	999:1022	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid)	999:1087	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	5	18	theme	oral	1572:1575	arg1	bioavailability					1577:1591	poor oral bioavailability	1567:1591	poor oral bioavailability	1567:1591	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	0	19	theme	Hydroxypropyl	78:90	arg1	Succinate					116:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	1	20	theme	oral	186:189	arg1	system					205:210	An advanced oral drug delivery system	174:210	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability	174:276	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability is strongly desirable.
28714596	1	20	theme	oral	186:189	arg1	desirable					290:298	desirable	290:298	desirable	290:298	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability is strongly desirable.
28714596	3	21	theme	stomach	903:909	arg1	conditions					885:894	simulated pH conditions	872:894	simulated pH conditions of the stomach	872:909	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	0	22	theme	Acetate	108:114	arg1	Succinate					116:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	4	23	with	interactions	1116:1127	arg1	intestines					1191:1200	the mouse small intestines	1175:1200	the mouse small intestines	1175:1200	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	4	23	with	interactions	1116:1127	arg1	cells					1165:1169	the intestinal Caco-2/HT29-MTX cells	1134:1169	the intestinal Caco-2/HT29-MTX cells	1134:1169	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	4	24	theme	mucoadhesive	1036:1047	arg1	poly					1049:1052	mucoadhesive poly	1036:1052	mucoadhesive poly(methyl vinyl ether-co-maleic acid)	1036:1087	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	4	24	theme	mucoadhesive	1036:1047	arg1	acid					1083:1086	methyl vinyl ether-co-maleic acid	1054:1086	methyl vinyl ether-co-maleic acid	1054:1086	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	4	25	theme	vinyl	1061:1065	arg1	poly					1049:1052	mucoadhesive poly	1036:1052	mucoadhesive poly(methyl vinyl ether-co-maleic acid)	1036:1087	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	4	25	theme	vinyl	1061:1065	arg1	acid					1083:1086	methyl vinyl ether-co-maleic acid	1054:1086	methyl vinyl ether-co-maleic acid	1054:1086	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	4	26	theme	co-cultured	1257:1267	arg1	monolayers					1290:1299	the co-cultured Caco-2/HT29-MTX cell monolayers	1253:1299	the co-cultured Caco-2/HT29-MTX cell monolayers	1253:1299	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	5	27	theme	vinyl	1425:1429	arg1	acid					1447:1450	methyl vinyl ether-co-maleic acid	1418:1450	methyl vinyl ether-co-maleic acid	1418:1450	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	5	27	theme	vinyl	1425:1429	arg1	poly					1413:1416	nanotube-mucoadhesive poly	1391:1416	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	2	28	theme	vinyl	424:428	arg1	acid					446:449	methyl vinyl ether-co-maleic acid	417:449	methyl vinyl ether-co-maleic acid	417:449	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	2	28	theme	vinyl	424:428	arg1	poly					412:415	the mucoadhesive poly	395:415	the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	395:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	0	29	theme	Multifunctional	0:14	arg1	Succinate					116:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	4	30	theme	cell	1285:1288	arg1	monolayers					1290:1299	the co-cultured Caco-2/HT29-MTX cell monolayers	1253:1299	the co-cultured Caco-2/HT29-MTX cell monolayers	1253:1299	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	5	31	theme	developed	1365:1373	arg1	composite					1501:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	5	31	theme	developed	1365:1373	arg1	system					1545:1550	a promising oral drug delivery system	1514:1550	a promising oral drug delivery system for drugs with poor oral bioavailability	1514:1591	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	2	32	theme	nanotubes	472:480	arg1	encapsulation					378:390	encapsulation	378:390	encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	378:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	0	33	theme	Poly	38:41	arg1	Succinate					116:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	1	34	with	drugs	241:245	arg1	bioavailability					262:276	poor oral bioavailability	252:276	poor oral bioavailability	252:276	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability is strongly desirable.
28714596	3	35	theme	pH	961:962	arg1	conditions					964:973	simulated pH conditions	951:973	simulated pH conditions of the small intestine	951:996	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	2	36	theme	methylcellulose	526:540	arg1	succinate					550:558	the pH-responsive hydroxypropyl methylcellulose acetate succinate	494:558	the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	494:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	3	37	theme	particle	728:735	arg1	µm					763:764	58 ± 1 µm	756:764	58 ± 1 µm	756:764	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	3	37	theme	particle	728:735	arg1	distribution					742:753	homogeneous particle size distribution	716:753	homogeneous particle size distribution (58 ± 1 µm)	716:765	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	5	38	theme	nanotube-mucoadhesive	1391:1411	arg1	acid					1447:1450	methyl vinyl ether-co-maleic acid	1418:1450	methyl vinyl ether-co-maleic acid	1418:1450	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	5	38	theme	nanotube-mucoadhesive	1391:1411	arg1	poly					1413:1416	nanotube-mucoadhesive poly	1391:1416	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	2	39	theme	modified	452:459	arg1	HNTs					483:486	HNTs	483:486	HNTs	483:486	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	2	39	theme	modified	452:459	arg1	nanotubes					472:480	the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes	395:480	the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	395:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	0	40	theme	methyl	43:48	arg1	Poly					38:41	Nanotube-Mucoadhesive Poly	16:41	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	0	40	theme	methyl	43:48	arg1	acid					72:75	methyl vinyl ether-co-maleic acid	43:75	methyl vinyl ether-co-maleic acid	43:75	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	2	41	theme	pH-responsive	498:510	arg1	succinate					550:558	the pH-responsive hydroxypropyl methylcellulose acetate succinate	494:558	the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	494:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	5	42	theme	methylcellulose	1467:1481	arg1	succinate					1491:1499	multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate	1375:1499	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	0	43	theme	ether-co-maleic	56:70	arg1	Poly					38:41	Nanotube-Mucoadhesive Poly	16:41	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	0	43	theme	ether-co-maleic	56:70	arg1	acid					72:75	methyl vinyl ether-co-maleic acid	43:75	methyl vinyl ether-co-maleic acid	43:75	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	4	44	with	modification	1011:1022	arg1	poly					1049:1052	mucoadhesive poly	1036:1052	mucoadhesive poly(methyl vinyl ether-co-maleic acid)	1036:1087	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	4	44	with	modification	1011:1022	arg1	acid					1083:1086	methyl vinyl ether-co-maleic acid	1054:1086	methyl vinyl ether-co-maleic acid	1054:1086	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	5	45	theme	succinate	1491:1499	arg1	composite					1501:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	5	45	theme	succinate	1491:1499	arg1	system					1545:1550	a promising oral drug delivery system	1514:1550	a promising oral drug delivery system for drugs with poor oral bioavailability	1514:1591	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	4	46	theme	intestinal	1138:1147	arg1	cells					1165:1169	the intestinal Caco-2/HT29-MTX cells	1134:1169	the intestinal Caco-2/HT29-MTX cells	1134:1169	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	1	47	theme	oral	257:260	arg1	bioavailability					262:276	poor oral bioavailability	252:276	poor oral bioavailability	252:276	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability is strongly desirable.
28714596	5	48	theme	@	1452:1452	arg1	succinate					1491:1499	multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate	1375:1499	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	2	49	theme	mucoadhesive	399:410	arg1	acid					446:449	methyl vinyl ether-co-maleic acid	417:449	methyl vinyl ether-co-maleic acid	417:449	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	2	49	theme	mucoadhesive	399:410	arg1	poly					412:415	the mucoadhesive poly	395:415	the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	395:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	5	50	theme	drug	1531:1534	arg1	composite					1501:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	5	50	theme	drug	1531:1534	arg1	system					1545:1550	a promising oral drug delivery system	1514:1550	a promising oral drug delivery system for drugs with poor oral bioavailability	1514:1591	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	2	51	theme	multifunctional	311:325	arg1	composite					352:360	a multifunctional nano-in-micro structured composite	309:360	a multifunctional nano-in-micro structured composite	309:360	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	4	52	theme	small	1185:1189	arg1	intestines					1191:1200	the mouse small intestines	1175:1200	the mouse small intestines	1175:1200	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	3	53	theme	dissolution	786:796	arg1	behavior					798:805	pH-responsive dissolution behavior	772:805	pH-responsive dissolution behavior	772:805	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	1	54	theme	drug	191:194	arg1	system					205:210	An advanced oral drug delivery system	174:210	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability	174:276	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability is strongly desirable.
28714596	1	54	theme	drug	191:194	arg1	desirable					290:298	desirable	290:298	desirable	290:298	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability is strongly desirable.
28714596	2	55	with	nanotubes	472:480	arg1	succinate					550:558	the pH-responsive hydroxypropyl methylcellulose acetate succinate	494:558	the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	494:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	0	56	theme	Oral	154:157	arg1	Delivery					164:171	Site-Specific Oral Drug Delivery	140:171	Site-Specific Oral Drug Delivery	140:171	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	5	57	theme	promising	1516:1524	arg1	composite					1501:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	5	57	theme	promising	1516:1524	arg1	system					1545:1550	a promising oral drug delivery system	1514:1550	a promising oral drug delivery system for drugs with poor oral bioavailability	1514:1591	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	2	58	theme	structured	341:350	arg1	composite					352:360	a multifunctional nano-in-micro structured composite	309:360	a multifunctional nano-in-micro structured composite	309:360	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	3	59	theme	small	982:986	arg1	intestine					988:996	the small intestine	978:996	the small intestine	978:996	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	2	60	theme	drug	596:599	arg1	release					601:607	the drug release	592:607	the drug release	592:607	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	4	61	theme	curcumin	1237:1244	arg1	permeability					1221:1232	the permeability	1217:1232	the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers	1217:1299	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	2	62	theme	cell-particle	619:631	arg1	interaction					633:643	cell-particle interaction	619:643	cell-particle interaction	619:643	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	3	63	from	curcumin	939:946	arg1	conditions					964:973	simulated pH conditions	951:973	simulated pH conditions of the small intestine	951:996	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	0	64	theme	@	77:77	arg1	Succinate					116:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	4	65	theme	surface	1003:1009	arg1	modification					1011:1022	The surface modification	999:1022	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid)	999:1087	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	0	66	theme	Methylcellulose	92:106	arg1	Succinate					116:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	1	67	theme	advanced	177:184	arg1	system					205:210	An advanced oral drug delivery system	174:210	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability	174:276	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability is strongly desirable.
28714596	1	67	theme	advanced	177:184	arg1	desirable					290:298	desirable	290:298	desirable	290:298	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability is strongly desirable.
28714596	2	68	theme	acetate	542:548	arg1	succinate					550:558	the pH-responsive hydroxypropyl methylcellulose acetate succinate	494:558	the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	494:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	3	69	theme	size	737:740	arg1	µm					763:764	58 ± 1 µm	756:764	58 ± 1 µm	756:764	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	3	69	theme	size	737:740	arg1	distribution					742:753	homogeneous particle size distribution	716:753	homogeneous particle size distribution (58 ± 1 µm)	716:765	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	3	70	theme	simulated	872:880	arg1	conditions					885:894	simulated pH conditions	872:894	simulated pH conditions of the stomach	872:909	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	5	71	theme	poly	1413:1416	arg1	succinate					1491:1499	multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate	1375:1499	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	3	72	theme	curcumin	860:867	arg1	release					849:855	the premature release	835:855	the premature release of curcumin	835:867	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	3	73	theme	simulated	951:959	arg1	conditions					964:973	simulated pH conditions	951:973	simulated pH conditions of the small intestine	951:996	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	4	74	theme	Caco-2/HT29-MTX	1269:1283	arg1	monolayers					1290:1299	the co-cultured Caco-2/HT29-MTX cell monolayers	1253:1299	the co-cultured Caco-2/HT29-MTX cell monolayers	1253:1299	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	5	75	theme	methyl	1418:1423	arg1	acid					1447:1450	methyl vinyl ether-co-maleic acid	1418:1450	methyl vinyl ether-co-maleic acid	1418:1450	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	5	75	theme	methyl	1418:1423	arg1	poly					1413:1416	nanotube-mucoadhesive poly	1391:1416	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	2	76	theme	ether-co-maleic	430:444	arg1	acid					446:449	methyl vinyl ether-co-maleic acid	417:449	methyl vinyl ether-co-maleic acid	417:449	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	2	76	theme	ether-co-maleic	430:444	arg1	poly					412:415	the mucoadhesive poly	395:415	the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	395:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	0	77	theme	Nanotube-Mucoadhesive	16:36	arg1	Poly					38:41	Nanotube-Mucoadhesive Poly	16:41	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	0	77	theme	Nanotube-Mucoadhesive	16:36	arg1	acid					72:75	methyl vinyl ether-co-maleic acid	43:75	methyl vinyl ether-co-maleic acid	43:75	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	4	78	theme	methyl	1054:1059	arg1	poly					1049:1052	mucoadhesive poly	1036:1052	mucoadhesive poly(methyl vinyl ether-co-maleic acid)	1036:1087	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	4	78	theme	methyl	1054:1059	arg1	acid					1083:1086	methyl vinyl ether-co-maleic acid	1054:1086	methyl vinyl ether-co-maleic acid	1054:1086	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	5	79	theme	poor	1567:1570	arg1	bioavailability					1577:1591	poor oral bioavailability	1567:1591	poor oral bioavailability	1567:1591	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	2	80	theme	methyl	417:422	arg1	acid					446:449	methyl vinyl ether-co-maleic acid	417:449	methyl vinyl ether-co-maleic acid	417:449	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	2	80	theme	methyl	417:422	arg1	poly					412:415	the mucoadhesive poly	395:415	the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	395:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	4	81	theme	ether-co-maleic	1067:1081	arg1	poly					1049:1052	mucoadhesive poly	1036:1052	mucoadhesive poly(methyl vinyl ether-co-maleic acid)	1036:1087	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	4	81	theme	ether-co-maleic	1067:1081	arg1	acid					1083:1086	methyl vinyl ether-co-maleic acid	1054:1086	methyl vinyl ether-co-maleic acid	1054:1086	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	3	82	theme	homogeneous	716:726	arg1	µm					763:764	58 ± 1 µm	756:764	58 ± 1 µm	756:764	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	3	82	theme	homogeneous	716:726	arg1	distribution					742:753	homogeneous particle size distribution	716:753	homogeneous particle size distribution (58 ± 1 µm)	716:765	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	5	83	theme	multifunctional	1375:1389	arg1	succinate					1491:1499	multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate	1375:1499	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	2	84	theme	halloysite	461:470	arg1	HNTs					483:486	HNTs	483:486	HNTs	483:486	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	2	84	theme	halloysite	461:470	arg1	nanotubes					472:480	the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes	395:480	the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	395:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	0	85	theme	vinyl	50:54	arg1	Poly					38:41	Nanotube-Mucoadhesive Poly	16:41	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate	0:124	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	0	85	theme	vinyl	50:54	arg1	acid					72:75	methyl vinyl ether-co-maleic acid	43:75	methyl vinyl ether-co-maleic acid	43:75	Multifunctional Nanotube-Mucoadhesive Poly(methyl vinyl ether-co-maleic acid)@Hydroxypropyl Methylcellulose Acetate Succinate Composite for Site-Specific Oral Drug Delivery.
28714596	2	86	theme	hydroxypropyl	512:524	arg1	succinate					550:558	the pH-responsive hydroxypropyl methylcellulose acetate succinate	494:558	the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	494:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	5	87	theme	hydroxypropyl	1453:1465	arg1	succinate					1491:1499	multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate	1375:1499	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	5	88	theme	acetate	1483:1489	arg1	succinate					1491:1499	multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate	1375:1499	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	3	89	theme	intestine	988:996	arg1	conditions					964:973	simulated pH conditions	951:973	simulated pH conditions of the small intestine	951:996	The microparticles show spherical shape, homogeneous particle size distribution (58 ± 1 µm), and pH-responsive dissolution behavior at pH > 6, and they prevent the premature release of curcumin in simulated pH conditions of the stomach and immediately release the curcumin in simulated pH conditions of the small intestine.
28714596	4	90	theme	free	1335:1338	arg1	curcumin					1340:1347	the free curcumin	1331:1347	the free curcumin	1331:1347	The surface modification of HNT with mucoadhesive poly(methyl vinyl ether-co-maleic acid) significantly enhances its interactions with the intestinal Caco-2/HT29-MTX cells and the mouse small intestines, and increases the permeability of curcumin across the co-cultured Caco-2/HT29-MTX cell monolayers by about 13 times compared to the free curcumin.
28714596	5	91	theme	ether-co-maleic	1431:1445	arg1	acid					1447:1450	methyl vinyl ether-co-maleic acid	1418:1450	methyl vinyl ether-co-maleic acid	1418:1450	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	5	91	theme	ether-co-maleic	1431:1445	arg1	poly					1413:1416	nanotube-mucoadhesive poly	1391:1416	the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite	1361:1509	Therefore, the developed multifunctional nanotube-mucoadhesive poly(methyl vinyl ether-co-maleic acid)@hydroxypropyl methylcellulose acetate succinate composite is a promising oral drug delivery system for drugs with poor oral bioavailability.
28714596	1	92	theme	poor	252:255	arg1	bioavailability					262:276	poor oral bioavailability	252:276	poor oral bioavailability	252:276	An advanced oral drug delivery system that can effectively deliver drugs with poor oral bioavailability is strongly desirable.
28714596	2	93	theme	poly	412:415	arg1	HNTs					483:486	HNTs	483:486	HNTs	483:486	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
28714596	2	93	theme	poly	412:415	arg1	nanotubes					472:480	the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes	395:480	the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption	395:672	Herein, a multifunctional nano-in-micro structured composite is developed by encapsulation of the mucoadhesive poly(methyl vinyl ether-co-maleic acid) modified halloysite nanotubes (HNTs) with the pH-responsive hydroxypropyl methylcellulose acetate succinate by the microfluidics to control the drug release, increase cell-particle interaction, and improve drug absorption.
26433644	1	0	theme	adsorbent	212:220	arg1	nanofiber					202:210	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	1	1	theme	dye	315:317	arg1	removal					295:301	removal	295:301	removal of cationic dye methylene blue from aqueous solution	295:354	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	0	2	theme	blue	118:121	arg1	methylene					108:116	methylene blue	108:121	methylene blue	108:121	Water-insoluble sericin/β-cyclodextrin/PVA composite electrospun nanofibers as effective adsorbents towards methylene blue.
26433644	8	3	theme	vinyl	1179:1183	arg1	alcohol					1185:1191	vinyl alcohol	1179:1191	vinyl alcohol	1179:1191	All these results suggest that crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers could be potential recyclable adsorbents in dye wastewater treatment.
26433644	8	4	theme	composite	1194:1202	arg1	nanofibers					1204:1213	crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers	1139:1213	crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers	1139:1213	All these results suggest that crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers could be potential recyclable adsorbents in dye wastewater treatment.
26433644	3	5	theme	methylene	636:644	arg1	adsorption					622:631	adsorption	622:631	adsorption of methylene blue	622:649	Kinetics, isotherms and thermodynamics analysis were studied for adsorption of methylene blue.
26433644	7	6	theme	adsorbent	1007:1015	arg1	membrane					998:1005	the fiber membrane adsorbent	988:1015	the fiber membrane adsorbent	988:1015	In addition, the fiber membrane adsorbent could be easily separated from dye solution and showed high recyclable removal efficiency.
26433644	1	7	theme	blue	329:332	arg1	dye					315:317	cationic dye	306:317	cationic dye methylene blue	306:332	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	7	8	theme	recyclable	1077:1086	arg1	efficiency					1096:1105	high recyclable removal efficiency	1072:1105	high recyclable removal efficiency	1072:1105	In addition, the fiber membrane adsorbent could be easily separated from dye solution and showed high recyclable removal efficiency.
26433644	2	9	dep	Fourier	357:363	arg1	transform					365:373	transform	365:373	transform infrared spectroscopy and solubility experiments	365:422	Fourier transform infrared spectroscopy and solubility experiments confirmed that sericin and β-cyclodextrin were incorporated into the nanofibers and the crosslinking reaction occurred successfully.
26433644	5	10	from	temperatures	832:843	arg1	261.10mg/g					814:823	261.10mg/g	814:823	261.10mg/g	814:823	The maximum adsorption capacities are 187.97, 229.89, and 261.10mg/g at the temperatures 293, 313 and 333 K, respectively.
26433644	5	10	from	temperatures	832:843	arg1	187.97					794:799	187.97	794:799	187.97	794:799	The maximum adsorption capacities are 187.97, 229.89, and 261.10mg/g at the temperatures 293, 313 and 333 K, respectively.
26433644	5	10	from	temperatures	832:843	arg1	capacities					779:788	The maximum adsorption capacities	756:788	The maximum adsorption capacities	756:788	The maximum adsorption capacities are 187.97, 229.89, and 261.10mg/g at the temperatures 293, 313 and 333 K, respectively.
26433644	5	10	from	temperatures	832:843	arg1	229.89					802:807	229.89	802:807	229.89	802:807	The maximum adsorption capacities are 187.97, 229.89, and 261.10mg/g at the temperatures 293, 313 and 333 K, respectively.
26433644	3	11	theme	thermodynamics	581:594	arg1	analysis					596:603	thermodynamics analysis	581:603	thermodynamics analysis	581:603	Kinetics, isotherms and thermodynamics analysis were studied for adsorption of methylene blue.
26433644	7	12	theme	fiber	992:996	arg1	membrane					998:1005	the fiber membrane adsorbent	988:1015	the fiber membrane adsorbent	988:1015	In addition, the fiber membrane adsorbent could be easily separated from dye solution and showed high recyclable removal efficiency.
26433644	4	13	theme	pseudo-second-order	701:719	arg1	model					721:725	the pseudo-second-order model	697:725	the pseudo-second-order model	697:725	The adsorption process is better fitted with the pseudo-second-order model and Langmuir isotherm model.
26433644	8	14	theme	crosslinked	1139:1149	arg1	nanofibers					1204:1213	crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers	1139:1213	crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers	1139:1213	All these results suggest that crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers could be potential recyclable adsorbents in dye wastewater treatment.
26433644	8	15	dep	sericin/β-cyclodextrin/poly	1151:1177	arg1	alcohol					1185:1191	vinyl alcohol	1179:1191	vinyl alcohol	1179:1191	All these results suggest that crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers could be potential recyclable adsorbents in dye wastewater treatment.
26433644	0	16	theme	sericin/β-cyclodextrin/PVA	16:41	arg1	electrospun					53:63	Water-insoluble sericin/β-cyclodextrin/PVA composite electrospun	0:63	Water-insoluble sericin/β-cyclodextrin/PVA composite electrospun	0:63	Water-insoluble sericin/β-cyclodextrin/PVA composite electrospun nanofibers as effective adsorbents towards methylene blue.
26433644	5	17	dep	temperatures	832:843	arg1	K					862:862	293, 313 and 333 K	845:862	293, 313 and 333 K	845:862	The maximum adsorption capacities are 187.97, 229.89, and 261.10mg/g at the temperatures 293, 313 and 333 K, respectively.
26433644	5	18	theme	adsorption	768:777	arg1	187.97					794:799	187.97	794:799	187.97	794:799	The maximum adsorption capacities are 187.97, 229.89, and 261.10mg/g at the temperatures 293, 313 and 333 K, respectively.
26433644	5	18	theme	adsorption	768:777	arg1	capacities					779:788	The maximum adsorption capacities	756:788	The maximum adsorption capacities	756:788	The maximum adsorption capacities are 187.97, 229.89, and 261.10mg/g at the temperatures 293, 313 and 333 K, respectively.
26433644	7	19	theme	high	1072:1075	arg1	efficiency					1096:1105	high recyclable removal efficiency	1072:1105	high recyclable removal efficiency	1072:1105	In addition, the fiber membrane adsorbent could be easily separated from dye solution and showed high recyclable removal efficiency.
26433644	0	20	theme	Water-insoluble	0:14	arg1	electrospun					53:63	Water-insoluble sericin/β-cyclodextrin/PVA composite electrospun	0:63	Water-insoluble sericin/β-cyclodextrin/PVA composite electrospun	0:63	Water-insoluble sericin/β-cyclodextrin/PVA composite electrospun nanofibers as effective adsorbents towards methylene blue.
26433644	1	21	theme	aqueous	339:345	arg1	solution					347:354	aqueous solution	339:354	aqueous solution	339:354	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	1	22	from	solution	347:354	arg1	removal					295:301	removal	295:301	removal of cationic dye methylene blue from aqueous solution	295:354	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	1	23	theme	novel	126:130	arg1	nanofiber					202:210	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	6	24	theme	blue	926:929	arg1	adsorption					931:940	methylene blue adsorption	916:940	methylene blue adsorption	916:940	Thermodynamic parameters showed that methylene blue adsorption was endothermic and spontaneous.
26433644	1	25	theme	water-insoluble	132:146	arg1	nanofiber					202:210	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	0	26	theme	composite	43:51	arg1	electrospun					53:63	Water-insoluble sericin/β-cyclodextrin/PVA composite electrospun	0:63	Water-insoluble sericin/β-cyclodextrin/PVA composite electrospun	0:63	Water-insoluble sericin/β-cyclodextrin/PVA composite electrospun nanofibers as effective adsorbents towards methylene blue.
26433644	8	27	theme	recyclable	1234:1243	arg1	adsorbents					1245:1254	potential recyclable adsorbents	1224:1254	potential recyclable adsorbents	1224:1254	All these results suggest that crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers could be potential recyclable adsorbents in dye wastewater treatment.
26433644	1	28	theme	sericin/β-cyclodextrin/poly	148:174	arg1	nanofiber					202:210	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	7	29	theme	removal	1088:1094	arg1	efficiency					1096:1105	high recyclable removal efficiency	1072:1105	high recyclable removal efficiency	1072:1105	In addition, the fiber membrane adsorbent could be easily separated from dye solution and showed high recyclable removal efficiency.
26433644	2	30	theme	crosslinking	512:523	arg1	reaction					525:532	the crosslinking reaction	508:532	the crosslinking reaction	508:532	Fourier transform infrared spectroscopy and solubility experiments confirmed that sericin and β-cyclodextrin were incorporated into the nanofibers and the crosslinking reaction occurred successfully.
26433644	2	31	dep	transform	365:373	arg1	infrared					375:382	infrared	375:382	transform infrared spectroscopy and solubility experiments	365:422	Fourier transform infrared spectroscopy and solubility experiments confirmed that sericin and β-cyclodextrin were incorporated into the nanofibers and the crosslinking reaction occurred successfully.
26433644	8	32	theme	dye	1259:1261	arg1	treatment					1274:1282	dye wastewater treatment	1259:1282	dye wastewater treatment	1259:1282	All these results suggest that crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers could be potential recyclable adsorbents in dye wastewater treatment.
26433644	1	33	theme	thermal	270:276	arg1	crosslinking					278:289	thermal crosslinking	270:289	thermal crosslinking	270:289	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	8	34	theme	sericin/β-cyclodextrin/poly	1151:1177	arg1	nanofibers					1204:1213	crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers	1139:1213	crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers	1139:1213	All these results suggest that crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers could be potential recyclable adsorbents in dye wastewater treatment.
26433644	6	35	theme	Thermodynamic	879:891	arg1	parameters					893:902	Thermodynamic parameters	879:902	Thermodynamic parameters	879:902	Thermodynamic parameters showed that methylene blue adsorption was endothermic and spontaneous.
26433644	8	36	link	crosslinked	1139:1149	arg1	nanofibers					1204:1213	crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers	1139:1213	crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers	1139:1213	All these results suggest that crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers could be potential recyclable adsorbents in dye wastewater treatment.
26433644	0	37	theme	effective	79:87	arg1	adsorbents					89:98	effective adsorbents	79:98	effective adsorbents	79:98	Water-insoluble sericin/β-cyclodextrin/PVA composite electrospun nanofibers as effective adsorbents towards methylene blue.
26433644	8	38	theme	wastewater	1263:1272	arg1	treatment					1274:1282	dye wastewater treatment	1259:1282	dye wastewater treatment	1259:1282	All these results suggest that crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers could be potential recyclable adsorbents in dye wastewater treatment.
26433644	5	39	theme	maximum	760:766	arg1	187.97					794:799	187.97	794:799	187.97	794:799	The maximum adsorption capacities are 187.97, 229.89, and 261.10mg/g at the temperatures 293, 313 and 333 K, respectively.
26433644	5	39	theme	maximum	760:766	arg1	capacities					779:788	The maximum adsorption capacities	756:788	The maximum adsorption capacities	756:788	The maximum adsorption capacities are 187.97, 229.89, and 261.10mg/g at the temperatures 293, 313 and 333 K, respectively.
26433644	1	40	theme	vinyl	177:181	arg1	nanofiber					202:210	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	4	41	theme	adsorption	656:665	arg1	process					667:673	The adsorption process	652:673	The adsorption process	652:673	The adsorption process is better fitted with the pseudo-second-order model and Langmuir isotherm model.
26433644	1	42	theme	alcohol	183:189	arg1	nanofiber					202:210	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	7	43	theme	dye	1048:1050	arg1	solution					1052:1059	dye solution	1048:1059	dye solution	1048:1059	In addition, the fiber membrane adsorbent could be easily separated from dye solution and showed high recyclable removal efficiency.
26433644	4	44	theme	isotherm	740:747	arg1	model					749:753	Langmuir isotherm model	731:753	Langmuir isotherm model	731:753	The adsorption process is better fitted with the pseudo-second-order model and Langmuir isotherm model.
26433644	2	45	dep	spectroscopy	384:395	arg1	experiments					412:422	experiments	412:422	experiments	412:422	Fourier transform infrared spectroscopy and solubility experiments confirmed that sericin and β-cyclodextrin were incorporated into the nanofibers and the crosslinking reaction occurred successfully.
26433644	4	46	theme	Langmuir	731:738	arg1	model					749:753	Langmuir isotherm model	731:753	Langmuir isotherm model	731:753	The adsorption process is better fitted with the pseudo-second-order model and Langmuir isotherm model.
26433644	1	47	theme	composite	192:200	arg1	nanofiber					202:210	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent	124:220	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
26433644	8	48	from	adsorbents	1245:1254	arg1	treatment					1274:1282	dye wastewater treatment	1259:1282	dye wastewater treatment	1259:1282	All these results suggest that crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers could be potential recyclable adsorbents in dye wastewater treatment.
26433644	8	49	theme	potential	1224:1232	arg1	adsorbents					1245:1254	potential recyclable adsorbents	1224:1254	potential recyclable adsorbents	1224:1254	All these results suggest that crosslinked sericin/β-cyclodextrin/poly(vinyl alcohol) composite nanofibers could be potential recyclable adsorbents in dye wastewater treatment.
26433644	3	50	theme	blue	646:649	arg1	methylene					636:644	methylene blue	636:649	methylene blue	636:649	Kinetics, isotherms and thermodynamics analysis were studied for adsorption of methylene blue.
26433644	1	51	theme	cationic	306:313	arg1	dye					315:317	cationic dye	306:317	cationic dye methylene blue	306:332	A novel water-insoluble sericin/β-cyclodextrin/poly (vinyl alcohol) composite nanofiber adsorbent was prepared by electrospinning and followed by thermal crosslinking for removal of cationic dye methylene blue from aqueous solution.
24783808	12	0	theme	Sialic	1322:1327	arg1	acid					1329:1332	Sialic acid	1322:1332	Sialic acid	1322:1332	Sialic acid is now well known as a cancer marker and investigations are on to use it as a drug-target in cancerous tissues.
24783808	12	0	theme	Sialic	1322:1327	arg1	it					1404:1405	it	1404:1405	it	1404:1405	Sialic acid is now well known as a cancer marker and investigations are on to use it as a drug-target in cancerous tissues.
24783808	12	0	theme	Sialic	1322:1327	arg1	marker					1364:1369	a cancer marker	1355:1369	a cancer marker	1355:1369	Sialic acid is now well known as a cancer marker and investigations are on to use it as a drug-target in cancerous tissues.
24783808	6	1	theme	total	737:741	arg1	glycoprotein					743:754	proteome total glycoprotein	728:754	proteome total glycoprotein	728:754	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	11	2	contain	had	1284:1286	arg2	effect					1291:1296	no effect	1288:1296	no effect	1288:1296	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	11	2	contain	had	1284:1286	arg1	Ginger					1186:1191	Ginger	1186:1191	Ginger	1186:1191	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	4	3	theme	extract	370:376	arg1	yield					354:358	A 10% yield	348:358	A 10% yield of ginger extract	348:376	A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C.
24783808	11	4	gly	glycoproteins	1307:1319	arg1	glycoproteins					1307:1319	other glycoproteins	1301:1319	other glycoproteins	1301:1319	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	11	5	theme	sialic	1228:1233	arg1	acid					1235:1238	O-linked sialic acid	1219:1238	O-linked sialic acid	1219:1238	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	9	6	theme	glycoprotein	1082:1093	arg1	kit					1095:1097	the total glycoprotein kit	1072:1097	the total glycoprotein kit	1072:1097	N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit.
24783808	4	7	theme	%	352:352	arg1	yield					354:358	A 10% yield	348:358	A 10% yield of ginger extract	348:376	A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C.
24783808	5	8	theme	Graphpad	602:609	arg1	software					617:624	Graphpad prism software	602:624	Graphpad prism software	602:624	Their half maximal inhibitory concentration (IC50) was determined and analyzed statistically using Graphpad prism software.
24783808	11	9	theme	N-linked	1249:1256	arg1	mannose					1258:1264	N-linked mannose	1249:1264	N-linked mannose	1249:1264	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	2	10	theme	anti-cancer	211:221	arg1	properties					239:248	anti-cancer and anti-fungal properties	211:248	anti-cancer and anti-fungal properties	211:248	The ginger rhizome is known to have anti-cancer and anti-fungal properties.
24783808	7	11	with	mL	862:863	arg1	significance					889:900	< 0.01 significance	882:900	< 0.01 significance	882:900	IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance.
24783808	8	12	from	staining	910:917	arg1	cells					953:957	Raji cells	948:957	Raji cells	948:957	Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans.
24783808	10	13	theme	galactose	1109:1117	arg1	acids					1139:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids were identified with the O-glycoprotein.
24783808	8	14	from	cells	953:957	arg1	staining					910:917	Silver staining	903:917	Silver staining of purified glycoprotiens in Raji cells	903:957	Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans.
24783808	4	15	theme	0.01	393:396	arg1	%					397:397	%	397:397	%	397:397	A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C.
24783808	0	16	theme	Raji	49:52	arg1	cells					54:58	Raji cells	49:58	Raji cells	49:58	The effect of ginger extract on glycoproteins of Raji cells.
24783808	2	17	theme	anti-fungal	227:237	arg1	properties					239:248	anti-cancer and anti-fungal properties	211:248	anti-cancer and anti-fungal properties	211:248	The ginger rhizome is known to have anti-cancer and anti-fungal properties.
24783808	1	18	theme	specific	137:144	arg1	diseases					146:153	specific diseases	137:153	specific diseases	137:153	Protein glycosylation is associated with the development and progression of specific diseases, including cancers.
24783808	1	18	theme	specific	137:144	arg1	cancers					166:172	cancers	166:172	cancers	166:172	Protein glycosylation is associated with the development and progression of specific diseases, including cancers.
24783808	10	19	link	O-linked	1100:1107	arg1	acids					1139:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids were identified with the O-glycoprotein.
24783808	7	20	theme	microg	855:860	arg1	-1					865:866	-1	865:866	-1	865:866	IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance.
24783808	7	20	theme	microg	855:860	arg1	mL					862:863	20 microg mL	852:863	20 microg mL(-1)	852:867	IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance.
24783808	6	21	gly	glycoproteins	665:677	arg1	glycoproteins					665:677	their glycoproteins	659:677	their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE	659:792	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	3	22	theme	cells	341:345	arg1	glycoproteins					319:331	glycoproteins	319:331	glycoproteins of Raji cells	319:345	This investigation was carried out to study the effect of ginger on glycoproteins of Raji cells.
24783808	4	23	theme	Raji	427:430	arg1	cells					432:436	6 x 10(4) Raji cells	417:436	6 x 10(4) Raji cells	417:436	A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C.
24783808	10	24	theme	O-linked	1123:1130	arg1	acids					1139:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids were identified with the O-glycoprotein.
24783808	11	25	from	expression	1205:1214	arg1	cells					1274:1278	Raji cells	1269:1278	Raji cells	1269:1278	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	6	26	gly	glycoprotein	762:773	arg1	glycoprotein					762:773	O glycoprotein	760:773	O glycoprotein	760:773	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	7	27	theme	Raji	837:840	arg1	cells					842:846	Raji cells	837:846	Raji cells	837:846	IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance.
24783808	1	28	theme	Protein	61:67	arg1	glycosylation					69:81	Protein glycosylation	61:81	Protein glycosylation	61:81	Protein glycosylation is associated with the development and progression of specific diseases, including cancers.
24783808	11	29	theme	other	1301:1305	arg1	glycoproteins					1307:1319	other glycoproteins	1301:1319	other glycoproteins	1301:1319	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	8	30	theme	purified	922:929	arg1	glycoprotiens					931:943	purified glycoprotiens	922:943	purified glycoprotiens in Raji cells	922:957	Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans.
24783808	7	31	theme	extract	826:832	arg1	IC50					811:814	IC50	811:814	IC50 of ginger extract on Raji cells	811:846	IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance.
24783808	6	32	dep	glycoprotein	743:754	arg1	kits					775:778	kits	775:778	kits	775:778	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	6	33	theme	SDS	785:787	arg1	PAGE					789:792	SDS PAGE	785:792	SDS PAGE	785:792	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	0	34	theme	extract	21:27	arg1	effect					4:9	The effect	0:9	The effect of ginger extract on glycoproteins of Raji cells	0:58	The effect of ginger extract on glycoproteins of Raji cells.
24783808	9	35	link	N-linked	1010:1017	arg1	acids					1047:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit.
24783808	5	36	theme	maximal	514:520	arg1	IC50					548:551	IC50	548:551	IC50	548:551	Their half maximal inhibitory concentration (IC50) was determined and analyzed statistically using Graphpad prism software.
24783808	5	36	theme	maximal	514:520	arg1	concentration					533:545	Their half maximal inhibitory concentration	503:545	Their half maximal inhibitory concentration (IC50)	503:552	Their half maximal inhibitory concentration (IC50) was determined and analyzed statistically using Graphpad prism software.
24783808	9	37	theme	N-linked	1010:1017	arg1	acids					1047:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit.
24783808	7	38	theme	ginger	819:824	arg1	extract					826:832	ginger extract	819:832	ginger extract	819:832	IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance.
24783808	4	39	theme	degrees	492:498	arg1	C					500:500	37 degrees C	489:500	37 degrees C	489:500	A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C.
24783808	11	40	theme	Raji	1269:1272	arg1	cells					1274:1278	Raji cells	1269:1278	Raji cells	1269:1278	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	2	41	theme	ginger	179:184	arg1	rhizome					186:192	The ginger rhizome	175:192	The ginger rhizome	175:192	The ginger rhizome is known to have anti-cancer and anti-fungal properties.
24783808	11	42	theme	mannose	1258:1264	arg1	expression					1205:1214	the expression	1201:1214	the expression of O-linked sialic acid and also N-linked mannose on Raji cells	1201:1278	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	9	43	theme	sialic	1040:1045	arg1	acids					1047:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit.
24783808	4	44	theme	ginger	363:368	arg1	extract					370:376	ginger extract	363:376	ginger extract	363:376	A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C.
24783808	9	45	theme	total	1076:1080	arg1	kit					1095:1097	the total glycoprotein kit	1072:1097	the total glycoprotein kit	1072:1097	N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit.
24783808	6	46	theme	proteome	728:735	arg1	glycoprotein					743:754	proteome total glycoprotein	728:754	proteome total glycoprotein	728:754	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	4	47	theme	10	350:351	arg1	%					352:352	%	352:352	%	352:352	A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C.
24783808	11	48	theme	O-linked	1219:1226	arg1	acid					1235:1238	O-linked sialic acid	1219:1238	O-linked sialic acid	1219:1238	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	5	49	theme	inhibitory	522:531	arg1	IC50					548:551	IC50	548:551	IC50	548:551	Their half maximal inhibitory concentration (IC50) was determined and analyzed statistically using Graphpad prism software.
24783808	5	49	theme	inhibitory	522:531	arg1	concentration					533:545	Their half maximal inhibitory concentration	503:545	Their half maximal inhibitory concentration (IC50)	503:552	Their half maximal inhibitory concentration (IC50) was determined and analyzed statistically using Graphpad prism software.
24783808	6	50	theme	lectin-affinity	694:708	arg1	Q					726:726	Q proteome total glycoprotein and O glycoprotein kits	726:778	Q proteome total glycoprotein and O glycoprotein kits	726:778	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	6	50	theme	lectin-affinity	694:708	arg1	chromatography					710:723	lectin-affinity chromatography	694:723	lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits)	694:779	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	6	51	dep	Q	726:726	arg1	glycoprotein					743:754	proteome total glycoprotein	728:754	proteome total glycoprotein	728:754	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	6	51	dep	Q	726:726	arg1	glycoprotein					762:773	O glycoprotein	760:773	O glycoprotein	760:773	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	11	52	link	N-linked	1249:1256	arg1	mannose					1258:1264	N-linked mannose	1249:1264	N-linked mannose	1249:1264	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	7	53	from	h	875:875	arg1	-1					865:866	-1	865:866	-1	865:866	IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance.
24783808	7	53	from	h	875:875	arg1	mL					862:863	20 microg mL	852:863	20 microg mL(-1)	852:867	IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance.
24783808	5	54	theme	prism	611:615	arg1	software					617:624	Graphpad prism software	602:624	Graphpad prism software	602:624	Their half maximal inhibitory concentration (IC50) was determined and analyzed statistically using Graphpad prism software.
24783808	8	55	from	glycoprotiens	931:943	arg1	cells					953:957	Raji cells	948:957	Raji cells	948:957	Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans.
24783808	11	56	theme	acid	1235:1238	arg1	expression					1205:1214	the expression	1201:1214	the expression of O-linked sialic acid and also N-linked mannose on Raji cells	1201:1278	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	9	57	gly	glycoprotein	1082:1093	arg1	glycoprotein					1082:1093	the total glycoprotein kit	1072:1097	the total glycoprotein kit	1072:1097	N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit.
24783808	10	58	gly	O-glycoprotein	1170:1183	arg1	O-glycoprotein					1170:1183	the O-glycoprotein	1166:1183	the O-glycoprotein	1166:1183	O-linked galactose and O-linked sialic acids were identified with the O-glycoprotein.
24783808	4	59	theme	%	397:397	arg1	DMSO					399:402	0.01% DMSO	393:402	0.01% DMSO	393:402	A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C.
24783808	10	60	theme	O-linked	1100:1107	arg1	acids					1139:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids were identified with the O-glycoprotein.
24783808	0	61	theme	cells	54:58	arg1	glycoproteins					32:44	glycoproteins	32:44	glycoproteins of Raji cells	32:58	The effect of ginger extract on glycoproteins of Raji cells.
24783808	12	62	from	drug-target	1412:1422	arg1	tissues					1437:1443	cancerous tissues	1427:1443	cancerous tissues	1427:1443	Sialic acid is now well known as a cancer marker and investigations are on to use it as a drug-target in cancerous tissues.
24783808	1	63	theme	diseases	146:153	arg1	progression					122:132	progression	122:132	progression	122:132	Protein glycosylation is associated with the development and progression of specific diseases, including cancers.
24783808	1	63	theme	diseases	146:153	arg1	development					106:116	development	106:116	development	106:116	Protein glycosylation is associated with the development and progression of specific diseases, including cancers.
24783808	3	64	theme	ginger	309:314	arg1	effect					299:304	the effect	295:304	the effect of ginger on glycoproteins of Raji cells	295:345	This investigation was carried out to study the effect of ginger on glycoproteins of Raji cells.
24783808	0	65	from	effect	4:9	arg1	glycoproteins					32:44	glycoproteins	32:44	glycoproteins of Raji cells	32:58	The effect of ginger extract on glycoproteins of Raji cells.
24783808	10	66	theme	sialic	1132:1137	arg1	acids					1139:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids were identified with the O-glycoprotein.
24783808	8	67	theme	Silver	903:908	arg1	staining					910:917	Silver staining	903:917	Silver staining of purified glycoprotiens in Raji cells	903:957	Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans.
24783808	3	68	theme	Raji	336:339	arg1	cells					341:345	Raji cells	336:345	Raji cells	336:345	This investigation was carried out to study the effect of ginger on glycoproteins of Raji cells.
24783808	10	69	link	O-linked	1123:1130	arg1	acids					1139:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids	1100:1143	O-linked galactose and O-linked sialic acids were identified with the O-glycoprotein.
24783808	1	70	dep	development	106:116	arg1	the					102:104	the	102:104	the	102:104	Protein glycosylation is associated with the development and progression of specific diseases, including cancers.
24783808	6	71	gly	glycoprotein	743:754	arg1	glycoprotein					743:754	proteome total glycoprotein	728:754	proteome total glycoprotein	728:754	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	8	72	theme	glycoprotiens	931:943	arg1	staining					910:917	Silver staining	903:917	Silver staining of purified glycoprotiens in Raji cells	903:957	Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans.
24783808	9	73	link	N-linked	1031:1038	arg1	acids					1047:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit.
24783808	7	74	from	IC50	811:814	arg1	cells					842:846	Raji cells	837:846	Raji cells	837:846	IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance.
24783808	0	75	theme	ginger	14:19	arg1	extract					21:27	ginger extract	14:27	ginger extract	14:27	The effect of ginger extract on glycoproteins of Raji cells.
24783808	0	76	gly	glycoproteins	32:44	arg1	glycoproteins					32:44	glycoproteins	32:44	glycoproteins of Raji cells	32:58	The effect of ginger extract on glycoproteins of Raji cells.
24783808	8	77	theme	Raji	948:951	arg1	cells					953:957	Raji cells	948:957	Raji cells	948:957	Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans.
24783808	4	78	dep	6	417:417	arg1	4					424:424	4	424:424	4	424:424	A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C.
24783808	12	79	theme	cancer	1357:1362	arg1	marker					1364:1369	a cancer marker	1355:1369	a cancer marker	1355:1369	Sialic acid is now well known as a cancer marker and investigations are on to use it as a drug-target in cancerous tissues.
24783808	12	79	theme	cancer	1357:1362	arg1	it					1404:1405	it	1404:1405	it	1404:1405	Sialic acid is now well known as a cancer marker and investigations are on to use it as a drug-target in cancerous tissues.
24783808	12	79	theme	cancer	1357:1362	arg1	acid					1329:1332	Sialic acid	1322:1332	Sialic acid	1322:1332	Sialic acid is now well known as a cancer marker and investigations are on to use it as a drug-target in cancerous tissues.
24783808	4	80	theme	different	441:449	arg1	concentrations					451:464	different concentrations	441:464	different concentrations for 24, 48 and 72 h	441:484	A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C.
24783808	12	81	theme	cancerous	1427:1435	arg1	tissues					1437:1443	cancerous tissues	1427:1443	cancerous tissues	1427:1443	Sialic acid is now well known as a cancer marker and investigations are on to use it as a drug-target in cancerous tissues.
24783808	3	82	gly	glycoproteins	319:331	arg1	glycoproteins					319:331	glycoproteins	319:331	glycoproteins of Raji cells	319:345	This investigation was carried out to study the effect of ginger on glycoproteins of Raji cells.
24783808	8	83	theme	O-glycans	985:993	arg1	presence					973:980	the presence	969:980	the presence of O-glycans and N-glycans	969:1007	Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans.
24783808	6	84	theme	O	760:760	arg1	glycoprotein					762:773	O glycoprotein	760:773	O glycoprotein	760:773	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	6	85	theme	Cell	627:630	arg1	extracts					632:639	Cell extracts	627:639	Cell extracts	627:639	Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out.
24783808	9	86	theme	mannose	1019:1025	arg1	acids					1047:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit.
24783808	2	87	contain	have	206:209	arg1	rhizome					186:192	The ginger rhizome	175:192	The ginger rhizome	175:192	The ginger rhizome is known to have anti-cancer and anti-fungal properties.
24783808	2	87	contain	have	206:209	arg2	properties					239:248	anti-cancer and anti-fungal properties	211:248	anti-cancer and anti-fungal properties	211:248	The ginger rhizome is known to have anti-cancer and anti-fungal properties.
24783808	8	88	theme	N-glycans	999:1007	arg1	presence					973:980	the presence	969:980	the presence of O-glycans and N-glycans	969:1007	Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans.
24783808	11	89	link	O-linked	1219:1226	arg1	acid					1235:1238	O-linked sialic acid	1219:1238	O-linked sialic acid	1219:1238	Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins.
24783808	9	90	theme	N-linked	1031:1038	arg1	acids					1047:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids	1010:1051	N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit.
24783808	3	91	from	effect	299:304	arg1	glycoproteins					319:331	glycoproteins	319:331	glycoproteins of Raji cells	319:345	This investigation was carried out to study the effect of ginger on glycoproteins of Raji cells.
27112884	4	0	theme	%	868:868	arg1	loading					875:881	5% clay loading	867:881	5% clay loading	867:881	Irrespective to the clay microstructure, an improvement in the mechanical properties of the chitosan-clay films has been substantiated with CS-SP reaching the highest value at 5% clay loading.
27112884	5	1	theme	hydrophilic	1100:1110	arg1	efficacy					1046:1053	the efficacy	1042:1053	the efficacy of the CO2 super-critical drying	1042:1086	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	5	1	theme	hydrophilic	1100:1110	arg1	face					1131:1134	the most hydrophilic CS-SP microspheres face	1091:1134	the most hydrophilic CS-SP microspheres face the highest shrinkage	1091:1156	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	10	2	theme	films	2164:2168	arg1	preparation					2098:2108	the preparation	2094:2108	the preparation of ultra-stable, practically-optimized food-packaging films and commercially scalable porous bio-based adsorbents	2094:2222	The knowledge gained from this rational design will constitute a guideline toward the preparation of ultra-stable, practically-optimized food-packaging films and commercially scalable porous bio-based adsorbents.
27112884	1	3	theme	microspheres	386:397	arg1	synthesis					322:330	the synthesis	318:330	the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres	318:397	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	7	4	theme	clay	1510:1513	arg1	particulates					1515:1526	the clay particulates	1506:1526	the clay particulates for the biopolymer	1506:1545	Under acidic environment, a longevity has been substantiated for chitosan-clay compared to native chitosan, evidencing the beneficial protective effect of the clay particulates for the biopolymer.
27112884	4	5	theme	highest	850:856	arg1	value					858:862	the highest value	846:862	the highest value	846:862	Irrespective to the clay microstructure, an improvement in the mechanical properties of the chitosan-clay films has been substantiated with CS-SP reaching the highest value at 5% clay loading.
27112884	10	6	theme	porous	2196:2201	arg1	adsorbents					2213:2222	commercially scalable porous bio-based adsorbents	2174:2222	commercially scalable porous bio-based adsorbents	2174:2222	The knowledge gained from this rational design will constitute a guideline toward the preparation of ultra-stable, practically-optimized food-packaging films and commercially scalable porous bio-based adsorbents.
27112884	6	7	theme	enhanced	1257:1264	arg1	properties					1274:1283	enhanced thermal properties	1257:1283	enhanced thermal properties	1257:1283	Chitosan-clay exhibits enhanced thermal properties with the degradation delayed in the order CS<CS-MMT<CS-HNT<CS-SP.
27112884	1	8	theme	clay-based	174:183	arg1	microstructures					185:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	8	theme	clay-based	174:183	arg1	sepiolite					289:297	micro-fibrillar sepiolite	273:297	micro-fibrillar sepiolite (SP)	273:302	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	8	theme	clay-based	174:183	arg1	montmorillonite					217:231	layered montmorillonite	209:231	layered montmorillonite (MMT)	209:237	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	8	theme	clay-based	174:183	arg1	halloysite					252:261	nanotubular halloysite	240:261	nanotubular halloysite (HNT)	240:267	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	7	9	theme	protective	1485:1494	arg1	effect					1496:1501	the beneficial protective effect	1470:1501	the beneficial protective effect of the clay particulates for the biopolymer	1470:1545	Under acidic environment, a longevity has been substantiated for chitosan-clay compared to native chitosan, evidencing the beneficial protective effect of the clay particulates for the biopolymer.
27112884	9	10	theme	CO2	1962:1964	arg1	drying					1970:1975	CO2 gel drying	1962:1975	CO2 gel drying	1962:1975	In this framework, a peculiar behavior was observed for CS-MMT, with the microspheres standing both against contraction during CO2 gel drying and under hydrothermal conditions.
27112884	4	11	theme	5	867:867	arg1	%					868:868	%	868:868	%	868:868	Irrespective to the clay microstructure, an improvement in the mechanical properties of the chitosan-clay films has been substantiated with CS-SP reaching the highest value at 5% clay loading.
27112884	10	12	dep	ultra-stable	2113:2124	arg1	practically-optimized					2127:2147	practically-optimized	2127:2147	practically-optimized	2127:2147	The knowledge gained from this rational design will constitute a guideline toward the preparation of ultra-stable, practically-optimized food-packaging films and commercially scalable porous bio-based adsorbents.
27112884	0	13	theme	films	111:115	arg1	stability					61:69	the stability	57:69	the stability of biomimetic hybrid chitosan-clay thin films	57:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	1	14	theme	layered	209:215	arg1	microstructures					185:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	14	theme	layered	209:215	arg1	MMT					234:236	MMT	234:236	MMT	234:236	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	14	theme	layered	209:215	arg1	montmorillonite					217:231	layered montmorillonite	209:231	layered montmorillonite (MMT)	209:237	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	0	15	from	stability	61:69	arg1	porous					131:136	CO2-dried porous	121:136	CO2-dried porous	121:136	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	0	15	from	stability	61:69	arg1	understanding					11:23	Insightful understanding	0:23	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films	0:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	10	16	theme	adsorbents	2213:2222	arg1	preparation					2098:2108	the preparation	2094:2108	the preparation of ultra-stable, practically-optimized food-packaging films and commercially scalable porous bio-based adsorbents	2094:2222	The knowledge gained from this rational design will constitute a guideline toward the preparation of ultra-stable, practically-optimized food-packaging films and commercially scalable porous bio-based adsorbents.
27112884	0	17	theme	CO2-dried	121:129	arg1	porous					131:136	CO2-dried porous	121:136	CO2-dried porous	121:136	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	1	18	theme	aerogel	378:384	arg1	microspheres					386:397	porous aerogel microspheres	371:397	porous aerogel microspheres	371:397	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	8	19	theme	hydrothermal	1563:1574	arg1	treatment					1576:1584	hydrothermal treatment	1563:1584	hydrothermal treatment	1563:1584	However, under hydrothermal treatment, the presence of clay was found to be detrimental to the material stability as a significant shrinkage occurs in hybrid CS-clay microspheres, which is attributed again to their increased hydrophilicity compared to the native polymeric microspheres.
27112884	2	20	theme	interaction	546:556	arg1	result					499:504	a result	497:504	a result of breaking the mutual polymeric chains interaction	497:556	At a first glance, a decrease in the viscosity of the three gel-forming solutions was noticed as a result of breaking the mutual polymeric chains interaction by the clay microstructure.
27112884	2	20	theme	interaction	546:556	arg1	decrease					421:428	a decrease	419:428	a decrease in the viscosity of the three gel-forming solutions	419:480	At a first glance, a decrease in the viscosity of the three gel-forming solutions was noticed as a result of breaking the mutual polymeric chains interaction by the clay microstructure.
27112884	4	21	theme	clay	711:714	arg1	microstructure					716:729	the clay microstructure	707:729	the clay microstructure	707:729	Irrespective to the clay microstructure, an improvement in the mechanical properties of the chitosan-clay films has been substantiated with CS-SP reaching the highest value at 5% clay loading.
27112884	8	22	theme	native	1804:1809	arg1	microspheres					1821:1832	the native polymeric microspheres	1800:1832	the native polymeric microspheres	1800:1832	However, under hydrothermal treatment, the presence of clay was found to be detrimental to the material stability as a significant shrinkage occurs in hybrid CS-clay microspheres, which is attributed again to their increased hydrophilicity compared to the native polymeric microspheres.
27112884	2	23	theme	polymeric	529:537	arg1	chains					539:544	the mutual polymeric chains	518:544	breaking the mutual polymeric chains interaction	509:556	At a first glance, a decrease in the viscosity of the three gel-forming solutions was noticed as a result of breaking the mutual polymeric chains interaction by the clay microstructure.
27112884	2	24	theme	solutions	472:480	arg1	viscosity					437:445	the viscosity	433:445	the viscosity of the three gel-forming solutions	433:480	At a first glance, a decrease in the viscosity of the three gel-forming solutions was noticed as a result of breaking the mutual polymeric chains interaction by the clay microstructure.
27112884	5	25	theme	CS-SP	1112:1116	arg1	efficacy					1046:1053	the efficacy	1042:1053	the efficacy of the CO2 super-critical drying	1042:1086	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	5	25	theme	CS-SP	1112:1116	arg1	face					1131:1134	the most hydrophilic CS-SP microspheres face	1091:1134	the most hydrophilic CS-SP microspheres face the highest shrinkage	1091:1156	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	0	26	theme	biomimetic	74:83	arg1	films					111:115	biomimetic hybrid chitosan-clay thin films	74:115	biomimetic hybrid chitosan-clay thin films	74:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	1	27	theme	hybrid	335:340	arg1	films					361:365	hybrid chitosan-clay thin films	335:365	hybrid chitosan-clay thin films	335:365	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	5	28	theme	native	957:962	arg1	chitosan					964:971	native chitosan	957:971	native chitosan	957:971	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	0	29	theme	chitosan-clay	92:104	arg1	films					111:115	biomimetic hybrid chitosan-clay thin films	74:115	biomimetic hybrid chitosan-clay thin films	74:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	1	30	theme	thin	356:359	arg1	films					361:365	hybrid chitosan-clay thin films	335:365	hybrid chitosan-clay thin films	335:365	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	7	31	theme	acidic	1357:1362	arg1	environment					1364:1374	acidic environment	1357:1374	acidic environment	1357:1374	Under acidic environment, a longevity has been substantiated for chitosan-clay compared to native chitosan, evidencing the beneficial protective effect of the clay particulates for the biopolymer.
27112884	0	32	from	role	32:35	arg1	stability					61:69	the stability	57:69	the stability of biomimetic hybrid chitosan-clay thin films	57:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	0	33	theme	Insightful	0:9	arg1	understanding					11:23	Insightful understanding	0:23	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films	0:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	4	34	from	improvement	735:745	arg1	properties					765:774	the mechanical properties	750:774	the mechanical properties of the chitosan-clay films	750:801	Irrespective to the clay microstructure, an improvement in the mechanical properties of the chitosan-clay films has been substantiated with CS-SP reaching the highest value at 5% clay loading.
27112884	2	35	theme	first	405:409	arg1	glance					411:416	a first glance	403:416	a first glance	403:416	At a first glance, a decrease in the viscosity of the three gel-forming solutions was noticed as a result of breaking the mutual polymeric chains interaction by the clay microstructure.
27112884	5	36	theme	lowest	1174:1179	arg1	area					1198:1201	a lowest specific surface area	1172:1201	a lowest specific surface area compared to CS-HNT and CS-MMT	1172:1231	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	9	37	theme	peculiar	1856:1863	arg1	behavior					1865:1872	a peculiar behavior	1854:1872	a peculiar behavior	1854:1872	In this framework, a peculiar behavior was observed for CS-MMT, with the microspheres standing both against contraction during CO2 gel drying and under hydrothermal conditions.
27112884	3	38	theme	enhanced	630:637	arg1	hydrophilicity					639:652	enhanced hydrophilicity	630:652	enhanced hydrophilicity	630:652	Upon casting, chitosan-clay films displayed enhanced hydrophilicity in the order CS<CS-MMT<CS-HNT<CS-SP.
27112884	0	39	theme	role	32:35	arg1	porous					131:136	CO2-dried porous	121:136	CO2-dried porous	121:136	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	0	39	theme	role	32:35	arg1	understanding					11:23	Insightful understanding	0:23	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films	0:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	5	40	theme	highest	1140:1146	arg1	shrinkage					1148:1156	the highest shrinkage	1136:1156	the most hydrophilic CS-SP microspheres face the highest shrinkage	1091:1156	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	8	41	theme	clay	1603:1606	arg1	presence					1591:1598	the presence	1587:1598	the presence of clay	1587:1606	However, under hydrothermal treatment, the presence of clay was found to be detrimental to the material stability as a significant shrinkage occurs in hybrid CS-clay microspheres, which is attributed again to their increased hydrophilicity compared to the native polymeric microspheres.
27112884	8	41	theme	clay	1603:1606	arg1	detrimental					1624:1634	detrimental	1624:1634	detrimental	1624:1634	However, under hydrothermal treatment, the presence of clay was found to be detrimental to the material stability as a significant shrinkage occurs in hybrid CS-clay microspheres, which is attributed again to their increased hydrophilicity compared to the native polymeric microspheres.
27112884	0	42	theme	clay	40:43	arg1	topology					45:52	clay topology	40:52	clay topology	40:52	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	3	43	theme	order	661:665	arg1	CS<CS-MMT<CS-HNT<CS-SP					667:688	the order CS<CS-MMT<CS-HNT<CS-SP	657:688	the order CS<CS-MMT<CS-HNT<CS-SP	657:688	Upon casting, chitosan-clay films displayed enhanced hydrophilicity in the order CS<CS-MMT<CS-HNT<CS-SP.
27112884	4	44	theme	films	797:801	arg1	properties					765:774	the mechanical properties	750:774	the mechanical properties of the chitosan-clay films	750:801	Irrespective to the clay microstructure, an improvement in the mechanical properties of the chitosan-clay films has been substantiated with CS-SP reaching the highest value at 5% clay loading.
27112884	8	45	theme	significant	1667:1677	arg1	shrinkage					1679:1687	a significant shrinkage	1665:1687	a significant shrinkage	1665:1687	However, under hydrothermal treatment, the presence of clay was found to be detrimental to the material stability as a significant shrinkage occurs in hybrid CS-clay microspheres, which is attributed again to their increased hydrophilicity compared to the native polymeric microspheres.
27112884	5	46	theme	enhanced	978:985	arg1	hydrophilicity					987:1000	the enhanced hydrophilicity	974:1000	the enhanced hydrophilicity associated to the water content	974:1032	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	7	47	theme	particulates	1515:1526	arg1	effect					1496:1501	the beneficial protective effect	1470:1501	the beneficial protective effect of the clay particulates for the biopolymer	1470:1545	Under acidic environment, a longevity has been substantiated for chitosan-clay compared to native chitosan, evidencing the beneficial protective effect of the clay particulates for the biopolymer.
27112884	8	48	theme	hybrid	1699:1704	arg1	microspheres					1714:1725	hybrid CS-clay microspheres	1699:1725	hybrid CS-clay microspheres	1699:1725	However, under hydrothermal treatment, the presence of clay was found to be detrimental to the material stability as a significant shrinkage occurs in hybrid CS-clay microspheres, which is attributed again to their increased hydrophilicity compared to the native polymeric microspheres.
27112884	5	49	theme	surface	1190:1196	arg1	area					1198:1201	a lowest specific surface area	1172:1201	a lowest specific surface area compared to CS-HNT and CS-MMT	1172:1231	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	2	50	theme	clay	565:568	arg1	microstructure					570:583	the clay microstructure	561:583	the clay microstructure	561:583	At a first glance, a decrease in the viscosity of the three gel-forming solutions was noticed as a result of breaking the mutual polymeric chains interaction by the clay microstructure.
27112884	10	51	theme	food-packaging	2149:2162	arg1	films					2164:2168	ultra-stable, practically-optimized food-packaging films	2113:2168	ultra-stable, practically-optimized food-packaging films	2113:2168	The knowledge gained from this rational design will constitute a guideline toward the preparation of ultra-stable, practically-optimized food-packaging films and commercially scalable porous bio-based adsorbents.
27112884	8	52	theme	material	1643:1650	arg1	stability					1652:1660	the material stability	1639:1660	the material stability	1639:1660	However, under hydrothermal treatment, the presence of clay was found to be detrimental to the material stability as a significant shrinkage occurs in hybrid CS-clay microspheres, which is attributed again to their increased hydrophilicity compared to the native polymeric microspheres.
27112884	4	53	theme	clay	870:873	arg1	loading					875:881	5% clay loading	867:881	5% clay loading	867:881	Irrespective to the clay microstructure, an improvement in the mechanical properties of the chitosan-clay films has been substantiated with CS-SP reaching the highest value at 5% clay loading.
27112884	10	54	theme	bio-based	2203:2211	arg1	adsorbents					2213:2222	commercially scalable porous bio-based adsorbents	2174:2222	commercially scalable porous bio-based adsorbents	2174:2222	The knowledge gained from this rational design will constitute a guideline toward the preparation of ultra-stable, practically-optimized food-packaging films and commercially scalable porous bio-based adsorbents.
27112884	6	55	theme	thermal	1266:1272	arg1	properties					1274:1283	enhanced thermal properties	1257:1283	enhanced thermal properties	1257:1283	Chitosan-clay exhibits enhanced thermal properties with the degradation delayed in the order CS<CS-MMT<CS-HNT<CS-SP.
27112884	1	56	theme	natural	166:172	arg1	microstructures					185:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	56	theme	natural	166:172	arg1	sepiolite					289:297	micro-fibrillar sepiolite	273:297	micro-fibrillar sepiolite (SP)	273:302	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	56	theme	natural	166:172	arg1	montmorillonite					217:231	layered montmorillonite	209:231	layered montmorillonite (MMT)	209:237	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	56	theme	natural	166:172	arg1	halloysite					252:261	nanotubular halloysite	240:261	nanotubular halloysite (HNT)	240:267	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	2	57	dep	breaking	509:516	arg1	chains					539:544	the mutual polymeric chains	518:544	breaking the mutual polymeric chains interaction	509:556	At a first glance, a decrease in the viscosity of the three gel-forming solutions was noticed as a result of breaking the mutual polymeric chains interaction by the clay microstructure.
27112884	7	58	theme	beneficial	1474:1483	arg1	effect					1496:1501	the beneficial protective effect	1470:1501	the beneficial protective effect of the clay particulates for the biopolymer	1470:1545	Under acidic environment, a longevity has been substantiated for chitosan-clay compared to native chitosan, evidencing the beneficial protective effect of the clay particulates for the biopolymer.
27112884	10	59	theme	scalable	2187:2194	arg1	adsorbents					2213:2222	commercially scalable porous bio-based adsorbents	2174:2222	commercially scalable porous bio-based adsorbents	2174:2222	The knowledge gained from this rational design will constitute a guideline toward the preparation of ultra-stable, practically-optimized food-packaging films and commercially scalable porous bio-based adsorbents.
27112884	2	60	from	decrease	421:428	arg1	viscosity					437:445	the viscosity	433:445	the viscosity of the three gel-forming solutions	433:480	At a first glance, a decrease in the viscosity of the three gel-forming solutions was noticed as a result of breaking the mutual polymeric chains interaction by the clay microstructure.
27112884	7	61	theme	native	1442:1447	arg1	chitosan					1449:1456	native chitosan	1442:1456	native chitosan	1442:1456	Under acidic environment, a longevity has been substantiated for chitosan-clay compared to native chitosan, evidencing the beneficial protective effect of the clay particulates for the biopolymer.
27112884	9	62	theme	gel	1966:1968	arg1	drying					1970:1975	CO2 gel drying	1962:1975	CO2 gel drying	1962:1975	In this framework, a peculiar behavior was observed for CS-MMT, with the microspheres standing both against contraction during CO2 gel drying and under hydrothermal conditions.
27112884	2	63	theme	breaking	509:516	arg1	interaction					546:556	breaking the mutual polymeric chains interaction	509:556	breaking the mutual polymeric chains interaction	509:556	At a first glance, a decrease in the viscosity of the three gel-forming solutions was noticed as a result of breaking the mutual polymeric chains interaction by the clay microstructure.
27112884	1	64	theme	porous	371:376	arg1	microspheres					386:397	porous aerogel microspheres	371:397	porous aerogel microspheres	371:397	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	8	65	theme	increased	1763:1771	arg1	hydrophilicity					1773:1786	their increased hydrophilicity	1757:1786	their increased hydrophilicity	1757:1786	However, under hydrothermal treatment, the presence of clay was found to be detrimental to the material stability as a significant shrinkage occurs in hybrid CS-clay microspheres, which is attributed again to their increased hydrophilicity compared to the native polymeric microspheres.
27112884	9	66	theme	hydrothermal	1987:1998	arg1	conditions					2000:2009	hydrothermal conditions	1987:2009	hydrothermal conditions	1987:2009	In this framework, a peculiar behavior was observed for CS-MMT, with the microspheres standing both against contraction during CO2 gel drying and under hydrothermal conditions.
27112884	6	67	theme	order	1321:1325	arg1	CS<CS-MMT<CS-HNT<CS-SP					1327:1348	the order CS<CS-MMT<CS-HNT<CS-SP	1317:1348	the order CS<CS-MMT<CS-HNT<CS-SP	1317:1348	Chitosan-clay exhibits enhanced thermal properties with the degradation delayed in the order CS<CS-MMT<CS-HNT<CS-SP.
27112884	5	68	theme	drying	1081:1086	arg1	efficacy					1046:1053	the efficacy	1042:1053	the efficacy of the CO2 super-critical drying	1042:1086	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	5	68	theme	drying	1081:1086	arg1	face					1131:1134	the most hydrophilic CS-SP microspheres face	1091:1134	the most hydrophilic CS-SP microspheres face the highest shrinkage	1091:1156	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	2	69	theme	mutual	522:527	arg1	chains					539:544	the mutual polymeric chains	518:544	breaking the mutual polymeric chains interaction	509:556	At a first glance, a decrease in the viscosity of the three gel-forming solutions was noticed as a result of breaking the mutual polymeric chains interaction by the clay microstructure.
27112884	8	70	theme	polymeric	1811:1819	arg1	microspheres					1821:1832	the native polymeric microspheres	1800:1832	the native polymeric microspheres	1800:1832	However, under hydrothermal treatment, the presence of clay was found to be detrimental to the material stability as a significant shrinkage occurs in hybrid CS-clay microspheres, which is attributed again to their increased hydrophilicity compared to the native polymeric microspheres.
27112884	2	71	theme	gel-forming	460:470	arg1	solutions					472:480	the three gel-forming solutions	450:480	the three gel-forming solutions	450:480	At a first glance, a decrease in the viscosity of the three gel-forming solutions was noticed as a result of breaking the mutual polymeric chains interaction by the clay microstructure.
27112884	10	72	theme	rational	2043:2050	arg1	design					2052:2057	this rational design	2038:2057	this rational design	2038:2057	The knowledge gained from this rational design will constitute a guideline toward the preparation of ultra-stable, practically-optimized food-packaging films and commercially scalable porous bio-based adsorbents.
27112884	5	73	theme	microspheres	1118:1129	arg1	efficacy					1046:1053	the efficacy	1042:1053	the efficacy of the CO2 super-critical drying	1042:1086	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	5	73	theme	microspheres	1118:1129	arg1	face					1131:1134	the most hydrophilic CS-SP microspheres face	1091:1134	the most hydrophilic CS-SP microspheres face the highest shrinkage	1091:1156	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	5	74	theme	clay	890:893	arg1	addition					895:902	clay addition	890:902	clay addition	890:902	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	0	75	from	understanding	11:23	arg1	stability					61:69	the stability	57:69	the stability of biomimetic hybrid chitosan-clay thin films	57:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	0	76	theme	hybrid	85:90	arg1	films					111:115	biomimetic hybrid chitosan-clay thin films	74:115	biomimetic hybrid chitosan-clay thin films	74:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	4	77	theme	mechanical	754:763	arg1	properties					765:774	the mechanical properties	750:774	the mechanical properties of the chitosan-clay films	750:801	Irrespective to the clay microstructure, an improvement in the mechanical properties of the chitosan-clay films has been substantiated with CS-SP reaching the highest value at 5% clay loading.
27112884	0	78	theme	thin	106:109	arg1	films					111:115	biomimetic hybrid chitosan-clay thin films	74:115	biomimetic hybrid chitosan-clay thin films	74:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	1	79	theme	chitosan-clay	342:354	arg1	films					361:365	hybrid chitosan-clay thin films	335:365	hybrid chitosan-clay thin films	335:365	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	80	theme	films	361:365	arg1	synthesis					322:330	the synthesis	318:330	the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres	318:397	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	5	81	dep	face	1131:1134	arg1	shrinkage					1148:1156	the highest shrinkage	1136:1156	the most hydrophilic CS-SP microspheres face the highest shrinkage	1091:1156	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	5	82	theme	CO2	1062:1064	arg1	drying					1081:1086	the CO2 super-critical drying	1058:1086	the CO2 super-critical drying	1058:1086	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	1	83	theme	micro-fibrillar	273:287	arg1	sepiolite					289:297	micro-fibrillar sepiolite	273:297	micro-fibrillar sepiolite (SP)	273:302	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	83	theme	micro-fibrillar	273:287	arg1	microstructures					185:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	83	theme	micro-fibrillar	273:287	arg1	SP					300:301	SP	300:301	SP	300:301	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	5	84	theme	specific	1181:1188	arg1	area					1198:1201	a lowest specific surface area	1172:1201	a lowest specific surface area compared to CS-HNT and CS-MMT	1172:1231	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	3	85	theme	chitosan-clay	600:612	arg1	films					614:618	chitosan-clay films	600:618	chitosan-clay films	600:618	Upon casting, chitosan-clay films displayed enhanced hydrophilicity in the order CS<CS-MMT<CS-HNT<CS-SP.
27112884	0	86	theme	topology	45:52	arg1	role					32:35	the role	28:35	the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films	28:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	5	87	theme	water	1020:1024	arg1	content					1026:1032	the water content	1016:1032	the water content	1016:1032	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	1	88	used	used	309:312	arg2	montmorillonite					217:231	layered montmorillonite	209:231	layered montmorillonite (MMT)	209:237	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	88	used	used	309:312	arg2	sepiolite					289:297	micro-fibrillar sepiolite	273:297	micro-fibrillar sepiolite (SP)	273:302	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	88	used	used	309:312	arg2	halloysite					252:261	nanotubular halloysite	240:261	nanotubular halloysite (HNT)	240:267	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	88	used	used	309:312	arg2	microstructures					185:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	5	89	theme	super-critical	1066:1079	arg1	drying					1081:1086	the CO2 super-critical drying	1058:1086	the CO2 super-critical drying	1058:1086	While clay addition provides a way to resist the shrinkage occurring for native chitosan, the enhanced hydrophilicity associated to the water content affects the efficacy of the CO2 super-critical drying as the most hydrophilic CS-SP microspheres face the highest shrinkage, resulting in a lowest specific surface area compared to CS-HNT and CS-MMT.
27112884	10	90	theme	ultra-stable	2113:2124	arg1	films					2164:2168	ultra-stable, practically-optimized food-packaging films	2113:2168	ultra-stable, practically-optimized food-packaging films	2113:2168	The knowledge gained from this rational design will constitute a guideline toward the preparation of ultra-stable, practically-optimized food-packaging films and commercially scalable porous bio-based adsorbents.
27112884	0	91	from	porous	131:136	arg1	stability					61:69	the stability	57:69	the stability of biomimetic hybrid chitosan-clay thin films	57:115	Insightful understanding of the role of clay topology on the stability of biomimetic hybrid chitosan-clay thin films and CO2-dried porous aerogel microspheres.
27112884	4	92	theme	chitosan-clay	783:795	arg1	films					797:801	the chitosan-clay films	779:801	the chitosan-clay films	779:801	Irrespective to the clay microstructure, an improvement in the mechanical properties of the chitosan-clay films has been substantiated with CS-SP reaching the highest value at 5% clay loading.
27112884	9	93	located	observed	1878:1885	arg2	behavior					1865:1872	a peculiar behavior	1854:1872	a peculiar behavior	1854:1872	In this framework, a peculiar behavior was observed for CS-MMT, with the microspheres standing both against contraction during CO2 gel drying and under hydrothermal conditions.
27112884	9	93	located	observed	1878:1885	arg1	framework					1843:1851	this framework	1838:1851	this framework	1838:1851	In this framework, a peculiar behavior was observed for CS-MMT, with the microspheres standing both against contraction during CO2 gel drying and under hydrothermal conditions.
27112884	8	94	theme	CS-clay	1706:1712	arg1	microspheres					1714:1725	hybrid CS-clay microspheres	1699:1725	hybrid CS-clay microspheres	1699:1725	However, under hydrothermal treatment, the presence of clay was found to be detrimental to the material stability as a significant shrinkage occurs in hybrid CS-clay microspheres, which is attributed again to their increased hydrophilicity compared to the native polymeric microspheres.
27112884	1	95	theme	nanotubular	240:250	arg1	microstructures					185:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures	160:199	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	95	theme	nanotubular	240:250	arg1	HNT					264:266	HNT	264:266	HNT	264:266	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27112884	1	95	theme	nanotubular	240:250	arg1	halloysite					252:261	nanotubular halloysite	240:261	nanotubular halloysite (HNT)	240:267	Three natural clay-based microstructures, namely layered montmorillonite (MMT), nanotubular halloysite (HNT) and micro-fibrillar sepiolite (SP) were used for the synthesis of hybrid chitosan-clay thin films and porous aerogel microspheres.
27188627	10	0	theme	organized	1362:1370	arg1	fibrils					1381:1387	more organized collagen fibrils	1357:1387	more organized collagen fibrils	1357:1387	The histological examination revealed more organized collagen fibrils in groups III, which showed a dense, parallel, linear organization of collagen bundles.
27188627	10	1	dep	groups	1392:1397	arg1	III					1399:1401	III	1399:1401	III	1399:1401	The histological examination revealed more organized collagen fibrils in groups III, which showed a dense, parallel, linear organization of collagen bundles.
27188627	1	2	theme	tendon	213:218	arg1	generation					220:229	new tendon generation	209:229	new tendon generation	209:229	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	2	3	theme	silk	419:422	arg1	scaffold					424:431	the composite silk scaffold	405:431	the composite silk scaffold	405:431	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	1	4	from	biocompatibility	131:146	arg1	terms					200:204	terms	200:204	terms of new tendon generation	200:229	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	2	5	theme	weaving	311:317	arg1	machine					319:325	a weaving machine	309:325	a weaving machine	309:325	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	1	6	theme	generation	220:229	arg1	terms					200:204	terms	200:204	terms of new tendon generation	200:229	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	4	7	from	scaffolds	690:698	arg1	cultures					719:726	30-day tenocyte cultures	703:726	30-day tenocyte cultures	703:726	The cellular densities on composite silk scaffolds were 40% higher on average than those on silk scaffolds in 30-day tenocyte cultures.
27188627	4	8	theme	cellular	597:604	arg1	higher					653:658	higher	653:658	higher	653:658	The cellular densities on composite silk scaffolds were 40% higher on average than those on silk scaffolds in 30-day tenocyte cultures.
27188627	4	8	theme	cellular	597:604	arg1	densities					606:614	The cellular densities	593:614	The cellular densities on composite silk scaffolds	593:642	The cellular densities on composite silk scaffolds were 40% higher on average than those on silk scaffolds in 30-day tenocyte cultures.
27188627	2	9	theme	silk	451:454	arg1	scaffold					456:463	a silk scaffold	449:463	a silk scaffold containing a lyophilized collagen-HA substrate	449:510	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	1	10	from	feasibility	115:125	arg1	terms					200:204	terms	200:204	terms of new tendon generation	200:229	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	4	11	theme	silk	685:688	arg1	scaffolds					690:698	silk scaffolds	685:698	silk scaffolds in 30-day tenocyte cultures	685:726	The cellular densities on composite silk scaffolds were 40% higher on average than those on silk scaffolds in 30-day tenocyte cultures.
27188627	2	12	contain	containing	465:474	arg2	substrate					502:510	a lyophilized collagen-HA substrate	476:510	a lyophilized collagen-HA substrate	476:510	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	2	12	contain	containing	465:474	arg1	scaffold					456:463	a silk scaffold	449:463	a silk scaffold containing a lyophilized collagen-HA substrate	449:510	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	6	13	theme	silk	898:901	arg1	group					889:893	group I	889:895	group I	889:895	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	13	theme	silk	898:901	arg1	scaffold					903:910	silk scaffold	898:910	silk scaffold	898:910	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	7	14	theme	12 weeks	1060:1067	arg1	post-implantation					1069:1085	12 weeks post-implantation	1060:1085	12 weeks post-implantation	1060:1085	Implants were harvested 2, 8, and 12 weeks post-implantation.
27188627	5	15	theme	Achilles	769:776	arg1	defects					785:791	Achilles tendon defects	769:791	Achilles tendon defects in 16 white New Zealand rabbits	769:823	The tendon scaffolds had implanted into Achilles tendon defects in 16 white New Zealand rabbits.
27188627	4	16	theme	30-day	703:708	arg1	cultures					719:726	30-day tenocyte cultures	703:726	30-day tenocyte cultures	703:726	The cellular densities on composite silk scaffolds were 40% higher on average than those on silk scaffolds in 30-day tenocyte cultures.
27188627	4	17	from	densities	606:614	arg1	scaffolds					634:642	composite silk scaffolds	619:642	composite silk scaffolds	619:642	The cellular densities on composite silk scaffolds were 40% higher on average than those on silk scaffolds in 30-day tenocyte cultures.
27188627	2	18	theme	1	345:345	arg1	%					346:346	%	346:346	%	346:346	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	10	19	theme	collagen	1459:1466	arg1	bundles					1468:1474	collagen bundles	1459:1474	collagen bundles	1459:1474	The histological examination revealed more organized collagen fibrils in groups III, which showed a dense, parallel, linear organization of collagen bundles.
27188627	2	20	theme	composite	409:417	arg1	scaffold					424:431	the composite silk scaffold	405:431	the composite silk scaffold	405:431	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	10	21	theme	histological	1323:1334	arg1	examination					1336:1346	The histological examination	1319:1346	The histological examination	1319:1346	The histological examination revealed more organized collagen fibrils in groups III, which showed a dense, parallel, linear organization of collagen bundles.
27188627	6	22	theme	silk	979:982	arg1	scaffold					984:991	composite silk scaffold	969:991	composite silk scaffold wrapped by an amniotic membrane	969:1023	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	22	theme	silk	979:982	arg1	group					958:962	group III	958:966	group III	958:966	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	1	23	theme	silk	153:156	arg1	scaffold					158:165	a silk scaffold	151:165	a silk scaffold	151:165	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	5	24	theme	tendon	733:738	arg1	scaffolds					740:748	The tendon scaffolds	729:748	The tendon scaffolds	729:748	The tendon scaffolds had implanted into Achilles tendon defects in 16 white New Zealand rabbits.
27188627	6	25	theme	composite	969:977	arg1	scaffold					984:991	composite silk scaffold	969:991	composite silk scaffold wrapped by an amniotic membrane	969:1023	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	25	theme	composite	969:977	arg1	group					958:962	group III	958:966	group III	958:966	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	11	26	theme	CD34	1477:1480	arg1	staining					1482:1489	CD34 staining	1477:1489	CD34 staining	1477:1489	CD34 staining revealed neoangiogenesis in groups III.
27188627	2	27	dep	constructed	291:301	arg1	whereas					397:403	whereas	397:403	whereas	397:403	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	12	28	theme	collagen-HA	1572:1582	arg1	substrates					1584:1593	collagen-HA substrates	1572:1593	collagen-HA substrates with amniotic membrane	1572:1616	The results of this research showed that collagen-HA substrates with amniotic membrane accelerate cellular migration and angiogenesis in neotendons.
27188627	1	29	theme	scaffold	158:165	arg1	biocompatibility					131:146	biocompatibility	131:146	biocompatibility	131:146	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	1	29	theme	scaffold	158:165	arg1	feasibility					115:125	feasibility	115:125	feasibility	115:125	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	1	30	theme	rabbit	239:244	arg1	model					262:266	a rabbit Achilles tendon model	237:266	a rabbit Achilles tendon model	237:266	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	10	31	theme	collagen	1372:1379	arg1	fibrils					1381:1387	more organized collagen fibrils	1357:1387	more organized collagen fibrils	1357:1387	The histological examination revealed more organized collagen fibrils in groups III, which showed a dense, parallel, linear organization of collagen bundles.
27188627	2	32	theme	silk	273:276	arg1	scaffold					278:285	The silk scaffold	269:285	The silk scaffold	269:285	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	5	33	theme	New	805:807	arg1	rabbits					817:823	16 white New Zealand rabbits	796:823	16 white New Zealand rabbits	796:823	The tendon scaffolds had implanted into Achilles tendon defects in 16 white New Zealand rabbits.
27188627	5	34	theme	tendon	778:783	arg1	defects					785:791	Achilles tendon defects	769:791	Achilles tendon defects in 16 white New Zealand rabbits	769:823	The tendon scaffolds had implanted into Achilles tendon defects in 16 white New Zealand rabbits.
27188627	1	35	theme	Achilles	246:253	arg1	model					262:266	a rabbit Achilles tendon model	237:266	a rabbit Achilles tendon model	237:266	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	2	36	theme	collagen-HA	490:500	arg1	substrate					502:510	a lyophilized collagen-HA substrate	476:510	a lyophilized collagen-HA substrate	476:510	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	1	37	dep	feasibility	115:125	arg1	the					111:113	the	111:113	the	111:113	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	12	38	with	substrates	1584:1593	arg1	membrane					1609:1616	amniotic membrane	1600:1616	amniotic membrane	1600:1616	The results of this research showed that collagen-HA substrates with amniotic membrane accelerate cellular migration and angiogenesis in neotendons.
27188627	2	39	theme	HA	369:370	arg1	solution					373:380	a 1% collagen-hyaluronan (HA) solution	343:380	a 1% collagen-hyaluronan (HA) solution	343:380	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	0	40	theme	silk	13:16	arg1	Co-effect					0:8	Co-effect	0:8	Co-effect of silk and amniotic membrane for tendon repair.	0:57	Co-effect of silk and amniotic membrane for tendon repair.
27188627	1	41	theme	tendon	255:260	arg1	model					262:266	a rabbit Achilles tendon model	237:266	a rabbit Achilles tendon model	237:266	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	2	42	theme	lyophilized	478:488	arg1	substrate					502:510	a lyophilized collagen-HA substrate	476:510	a lyophilized collagen-HA substrate	476:510	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	1	43	theme	study	88:92	arg1	objective					63:71	The objective	59:71	The objective of the present study	59:92	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	12	44	theme	research	1551:1558	arg1	results					1535:1541	The results	1531:1541	The results of this research	1531:1558	The results of this research showed that collagen-HA substrates with amniotic membrane accelerate cellular migration and angiogenesis in neotendons.
27188627	1	45	theme	composite	173:181	arg1	scaffold					188:195	a composite silk scaffold	171:195	a composite silk scaffold	171:195	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	0	46	theme	membrane	31:38	arg1	Co-effect					0:8	Co-effect	0:8	Co-effect of silk and amniotic membrane for tendon repair.	0:57	Co-effect of silk and amniotic membrane for tendon repair.
27188627	2	47	theme	collagen-hyaluronan	348:366	arg1	solution					373:380	a 1% collagen-hyaluronan (HA) solution	343:380	a 1% collagen-hyaluronan (HA) solution	343:380	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	12	48	from	angiogenesis	1652:1663	arg1	neotendons					1668:1677	neotendons	1668:1677	neotendons	1668:1677	The results of this research showed that collagen-HA substrates with amniotic membrane accelerate cellular migration and angiogenesis in neotendons.
27188627	1	49	theme	silk	183:186	arg1	scaffold					188:195	a composite silk scaffold	171:195	a composite silk scaffold	171:195	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	0	50	theme	amniotic	22:29	arg1	membrane					31:38	amniotic membrane	22:38	amniotic membrane	22:38	Co-effect of silk and amniotic membrane for tendon repair.
27188627	10	51	from	fibrils	1381:1387	arg1	groups					1392:1397	groups III	1392:1401	groups III	1392:1401	The histological examination revealed more organized collagen fibrils in groups III, which showed a dense, parallel, linear organization of collagen bundles.
27188627	5	52	theme	white	799:803	arg1	rabbits					817:823	16 white New Zealand rabbits	796:823	16 white New Zealand rabbits	796:823	The tendon scaffolds had implanted into Achilles tendon defects in 16 white New Zealand rabbits.
27188627	6	53	dep	groups	881:886	arg1	scaffold					944:951	composite silk scaffold	929:951	composite silk scaffold	929:951	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	53	dep	groups	881:886	arg1	group					889:893	group I	889:895	group I	889:895	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	53	dep	groups	881:886	arg1	scaffold					984:991	composite silk scaffold	969:991	composite silk scaffold wrapped by an amniotic membrane	969:1023	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	53	dep	groups	881:886	arg1	group					919:923	group II	919:926	group II	919:926	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	53	dep	groups	881:886	arg1	group					958:962	group III	958:966	group III	958:966	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	53	dep	groups	881:886	arg1	groups					881:886	the following three groups	861:886	the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane	861:1023	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	53	dep	groups	881:886	arg1	scaffold					903:910	silk scaffold	898:910	silk scaffold	898:910	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	8	54	theme	Histological	1088:1099	arg1	examinations					1101:1112	Histological examinations	1088:1112	Histological examinations	1088:1112	Histological examinations were conducted using hematoxylin-eosin (H&E), Masson's trichrome, and by performing immunohistochemical staining for CD34.
27188627	1	55	theme	scaffold	188:195	arg1	biocompatibility					131:146	biocompatibility	131:146	biocompatibility	131:146	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	1	55	theme	scaffold	188:195	arg1	feasibility					115:125	feasibility	115:125	feasibility	115:125	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	0	56	theme	tendon	44:49	arg1	repair					51:56	tendon repair	44:56	tendon repair	44:56	Co-effect of silk and amniotic membrane for tendon repair.
27188627	5	57	theme	Zealand	809:815	arg1	rabbits					817:823	16 white New Zealand rabbits	796:823	16 white New Zealand rabbits	796:823	The tendon scaffolds had implanted into Achilles tendon defects in 16 white New Zealand rabbits.
27188627	6	58	theme	silk	939:942	arg1	scaffold					944:951	composite silk scaffold	929:951	composite silk scaffold	929:951	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	58	theme	silk	939:942	arg1	group					919:923	group II	919:926	group II	919:926	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	12	59	from	migration	1638:1646	arg1	neotendons					1668:1677	neotendons	1668:1677	neotendons	1668:1677	The results of this research showed that collagen-HA substrates with amniotic membrane accelerate cellular migration and angiogenesis in neotendons.
27188627	5	60	from	defects	785:791	arg1	rabbits					817:823	16 white New Zealand rabbits	796:823	16 white New Zealand rabbits	796:823	The tendon scaffolds had implanted into Achilles tendon defects in 16 white New Zealand rabbits.
27188627	6	61	theme	following	865:873	arg1	group					889:893	group I	889:895	group I	889:895	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	61	theme	following	865:873	arg1	groups					881:886	the following three groups	861:886	the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane	861:1023	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	61	theme	following	865:873	arg1	group					958:962	group III	958:966	group III	958:966	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	61	theme	following	865:873	arg1	group					919:923	group II	919:926	group II	919:926	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	10	62	theme	dense	1419:1423	arg1	organization					1443:1454	a dense, parallel, linear organization	1417:1454	a dense, parallel, linear organization of collagen bundles	1417:1474	The histological examination revealed more organized collagen fibrils in groups III, which showed a dense, parallel, linear organization of collagen bundles.
27188627	6	63	theme	amniotic	1007:1014	arg1	membrane					1016:1023	an amniotic membrane	1004:1023	an amniotic membrane	1004:1023	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	1	64	theme	present	80:86	arg1	study					88:92	the present study	76:92	the present study	76:92	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27188627	6	65	theme	composite	929:937	arg1	scaffold					944:951	composite silk scaffold	929:951	composite silk scaffold	929:951	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	6	65	theme	composite	929:937	arg1	group					919:923	group II	919:926	group II	919:926	Rabbits were randomly divided into the following three groups: group I, silk scaffold alone; group II, composite silk scaffold; and group III, composite silk scaffold wrapped by an amniotic membrane.
27188627	4	66	theme	tenocyte	710:717	arg1	cultures					719:726	30-day tenocyte cultures	703:726	30-day tenocyte cultures	703:726	The cellular densities on composite silk scaffolds were 40% higher on average than those on silk scaffolds in 30-day tenocyte cultures.
27188627	10	67	theme	bundles	1468:1474	arg1	organization					1443:1454	a dense, parallel, linear organization	1417:1454	a dense, parallel, linear organization of collagen bundles	1417:1474	The histological examination revealed more organized collagen fibrils in groups III, which showed a dense, parallel, linear organization of collagen bundles.
27188627	8	68	theme	immunohistochemical	1198:1216	arg1	staining					1218:1225	immunohistochemical staining	1198:1225	immunohistochemical staining for CD34	1198:1234	Histological examinations were conducted using hematoxylin-eosin (H&E), Masson's trichrome, and by performing immunohistochemical staining for CD34.
27188627	10	69	dep	dense	1419:1423	arg1	linear					1436:1441	linear	1436:1441	linear	1436:1441	The histological examination revealed more organized collagen fibrils in groups III, which showed a dense, parallel, linear organization of collagen bundles.
27188627	10	69	dep	dense	1419:1423	arg1	parallel					1426:1433	parallel	1426:1433	parallel	1426:1433	The histological examination revealed more organized collagen fibrils in groups III, which showed a dense, parallel, linear organization of collagen bundles.
27188627	2	70	theme	%	346:346	arg1	solution					373:380	a 1% collagen-hyaluronan (HA) solution	343:380	a 1% collagen-hyaluronan (HA) solution	343:380	The silk scaffold was constructed using a weaving machine, then soaked in a 1% collagen-hyaluronan (HA) solution and air-dried, whereas the composite silk scaffold was composed of a silk scaffold containing a lyophilized collagen-HA substrate.
27188627	12	71	theme	amniotic	1600:1607	arg1	membrane					1609:1616	amniotic membrane	1600:1616	amniotic membrane	1600:1616	The results of this research showed that collagen-HA substrates with amniotic membrane accelerate cellular migration and angiogenesis in neotendons.
27188627	4	72	theme	silk	629:632	arg1	scaffolds					634:642	composite silk scaffolds	619:642	composite silk scaffolds	619:642	The cellular densities on composite silk scaffolds were 40% higher on average than those on silk scaffolds in 30-day tenocyte cultures.
27188627	12	73	theme	cellular	1629:1636	arg1	migration					1638:1646	cellular migration	1629:1646	cellular migration	1629:1646	The results of this research showed that collagen-HA substrates with amniotic membrane accelerate cellular migration and angiogenesis in neotendons.
27188627	3	74	theme	cell	557:560	arg1	populations					562:572	cell populations	557:572	cell populations	557:572	Tenocytes were cultured in vitro to compare cell populations in the two groups.
27188627	4	75	theme	composite	619:627	arg1	scaffolds					634:642	composite silk scaffolds	619:642	composite silk scaffolds	619:642	The cellular densities on composite silk scaffolds were 40% higher on average than those on silk scaffolds in 30-day tenocyte cultures.
27188627	9	76	theme	gross	1300:1304	arg1	examination					1306:1316	gross examination	1300:1316	gross examination	1300:1316	After 12 weeks, the three groups were distinguishable based on gross examination.
27188627	1	77	theme	new	209:211	arg1	generation					220:229	new tendon generation	209:229	new tendon generation	209:229	The objective of the present study was to determine the feasibility and biocompatibility of a silk scaffold and a composite silk scaffold in terms of new tendon generation using a rabbit Achilles tendon model.
27644050	9	0	theme	Unweighted	1084:1093	arg1	distances					1103:1111	Unweighted UniFrac distances	1084:1111	Unweighted UniFrac distances	1084:1111	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	3	1	theme	inactive	468:475	arg1	genes					486:490	inactive secretor genes	468:490	inactive secretor genes	468:490	However, mothers with inactive secretor genes have altered HMO composition and quantities in their breast milk.
27644050	6	2	theme	microbiota	867:876	arg1	composition					878:888	faecal microbiota composition	860:888	faecal microbiota composition	860:888	Secretor status was determined from blood and saliva samples using hemagglutination inhibition technique and faecal microbiota composition was examined by 16S rRNA gene sequencing.
27644050	11	3	theme	sulphated	1793:1801	arg1	polysaccharides					1803:1817	sulphated polysaccharides	1793:1817	sulphated polysaccharides for growth	1793:1828	The relative abundance of an OTU related to Bacteroides plebeius, a bacterium noted for its capacity to utilise sulphated polysaccharides for growth, was decreased in these children.
27644050	6	4	theme	Secretor	751:758	arg1	status					760:765	Secretor status	751:765	Secretor status	751:765	Secretor status was determined from blood and saliva samples using hemagglutination inhibition technique and faecal microbiota composition was examined by 16S rRNA gene sequencing.
27644050	9	5	theme	=	1297:1297	arg1	0.028					1299:1303	p = 0.028	1295:1303	p = 0.028	1295:1303	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	4	6	theme	status	615:620	arg1	influence					593:601	the influence	589:601	the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age	589:688	In this pilot study we examine the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age.
27644050	3	7	theme	altered	497:503	arg1	composition					509:519	altered HMO composition	497:519	altered HMO composition	497:519	However, mothers with inactive secretor genes have altered HMO composition and quantities in their breast milk.
27644050	6	8	theme	inhibition	835:844	arg1	technique					846:854	hemagglutination inhibition technique	818:854	hemagglutination inhibition technique	818:854	Secretor status was determined from blood and saliva samples using hemagglutination inhibition technique and faecal microbiota composition was examined by 16S rRNA gene sequencing.
27644050	4	9	theme	breast-feeding	626:639	arg1	influence					593:601	the influence	589:601	the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age	589:688	In this pilot study we examine the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age.
27644050	3	10	with	mothers	455:461	arg1	genes					486:490	inactive secretor genes	468:490	inactive secretor genes	468:490	However, mothers with inactive secretor genes have altered HMO composition and quantities in their breast milk.
27644050	2	11	theme	nutrition	291:299	arg1	milk					325:328	breast milk	318:328	breast milk	318:328	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	2	11	theme	nutrition	291:299	arg1	source					281:286	the optimal source	269:286	the optimal source of nutrition in early life	269:313	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	1	12	theme	term	185:188	arg1	health					190:195	long term health	180:195	long term health	180:195	BACKGROUND One mechanism by which early life environment may influence long term health is through modulation of the gut microbiota.
27644050	12	13	contain	have	1911:1914	arg1	CONCLUSIONS					1864:1874	CONCLUSIONS Child and mothers' secretor status	1864:1909	CONCLUSIONS Child and mothers' secretor status	1864:1909	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	12	13	contain	have	1911:1914	arg2	impact					1919:1924	an impact	1916:1924	an impact on childrens' microbiota composition	1916:1961	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	12	13	contain	have	1911:1914	arg1	Child					1876:1880	Child	1876:1880	Child	1876:1880	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	12	13	contain	have	1911:1914	arg1	status					1904:1909	mothers' secretor status	1886:1909	mothers' secretor status	1886:1909	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	9	14	theme	secretor	1154:1161	arg1	0.069					1176:1180	R2 = 0.069	1171:1180	R2 = 0.069	1171:1180	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	14	theme	secretor	1154:1161	arg1	status					1163:1168	child secretor status	1148:1168	child secretor status (R2 = 0.069, p = 0.030)	1148:1192	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	10	15	theme	known	1560:1564	arg1	consumers					1570:1578	the known HMO consumers	1556:1578	the known HMO consumers	1556:1578	In children who were exclusively breast-fed for at least 4 months of life the abundance of the known HMO consumers Bifidobacterium were increased in the children of secretor mothers compared to non-secretor mothers.
27644050	11	16	theme	relative	1685:1692	arg1	abundance					1694:1702	The relative abundance	1681:1702	The relative abundance of an OTU related to Bacteroides plebeius, a bacterium noted for its capacity to utilise sulphated polysaccharides for growth,	1681:1829	The relative abundance of an OTU related to Bacteroides plebeius, a bacterium noted for its capacity to utilise sulphated polysaccharides for growth, was decreased in these children.
27644050	10	17	theme	non-secretor	1659:1670	arg1	mothers					1672:1678	non-secretor mothers	1659:1678	non-secretor mothers	1659:1678	In children who were exclusively breast-fed for at least 4 months of life the abundance of the known HMO consumers Bifidobacterium were increased in the children of secretor mothers compared to non-secretor mothers.
27644050	6	18	theme	rRNA	910:913	arg1	sequencing					920:929	16S rRNA gene sequencing	906:929	16S rRNA gene sequencing	906:929	Secretor status was determined from blood and saliva samples using hemagglutination inhibition technique and faecal microbiota composition was examined by 16S rRNA gene sequencing.
27644050	9	19	theme	=	1174:1174	arg1	0.069					1176:1180	R2 = 0.069	1171:1180	R2 = 0.069	1171:1180	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	19	theme	=	1174:1174	arg1	status					1163:1168	child secretor status	1148:1168	child secretor status (R2 = 0.069, p = 0.030)	1148:1192	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	20	from	children	1435:1442	arg1	samples					1400:1406	samples	1400:1406	samples from secretor children and children of secretor mothers	1400:1462	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	21	located	detected	1388:1395	arg2	Prevotella					1373:1382	Prevotella	1373:1382	Prevotella	1373:1382	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	21	located	detected	1388:1395	arg1	samples					1400:1406	samples	1400:1406	samples from secretor children and children of secretor mothers	1400:1462	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	7	22	theme	being	1004:1008	arg1	secretors					1010:1018	20 being secretors	1001:1018	20 being secretors (S, 71.4%)	1001:1029	RESULTS Secretor status was determined for 28 eligible children with 20 being secretors (S, 71.4%).
27644050	7	22	theme	being	1004:1008	arg1	S					1021:1021	S	1021:1021	S	1021:1021	RESULTS Secretor status was determined for 28 eligible children with 20 being secretors (S, 71.4%).
27644050	3	23	theme	HMO	505:507	arg1	composition					509:519	altered HMO composition	497:519	altered HMO composition	497:519	However, mothers with inactive secretor genes have altered HMO composition and quantities in their breast milk.
27644050	9	24	from	children	1422:1429	arg1	samples					1400:1406	samples	1400:1406	samples from secretor children and children of secretor mothers	1400:1462	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	4	25	theme	microbiota	644:653	arg1	composition					655:665	microbiota composition	644:665	microbiota composition at 2 to 3 years of age	644:688	In this pilot study we examine the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age.
27644050	0	26	theme	Composition	68:78	arg1	Development					32:42	Development	32:42	Development of Childrens Microbiota Composition and Function	32:91	Mothers Secretor Status Affects Development of Childrens Microbiota Composition and Function: A Pilot Study.
27644050	9	27	theme	=	1185:1185	arg1	p					1183:1183	p = 0.030	1183:1191	p = 0.030	1183:1191	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	1	28	theme	early	143:147	arg1	environment					154:164	early life environment	143:164	early life environment	143:164	BACKGROUND One mechanism by which early life environment may influence long term health is through modulation of the gut microbiota.
27644050	0	29	theme	Function	84:91	arg1	Development					32:42	Development	32:42	Development of Childrens Microbiota Composition and Function	32:91	Mothers Secretor Status Affects Development of Childrens Microbiota Composition and Function: A Pilot Study.
27644050	7	30	theme	RESULTS	932:938	arg1	status					949:954	RESULTS Secretor status	932:954	RESULTS Secretor status	932:954	RESULTS Secretor status was determined for 28 eligible children with 20 being secretors (S, 71.4%).
27644050	9	31	with	microbes	1358:1365	arg1	Prevotella					1373:1382	Prevotella	1373:1382	Prevotella	1373:1382	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	5	32	theme	METHODS	691:697	arg1	children					702:709	METHODS 37 children	691:709	METHODS 37 children	691:709	METHODS 37 children and 17 eligible mothers were recruited.
27644050	9	33	theme	secretor	1210:1217	arg1	status					1219:1224	mother secretor status	1203:1224	mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028)	1203:1304	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	34	theme	secretor	1413:1420	arg1	children					1422:1429	secretor children	1413:1429	secretor children	1413:1429	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	4	35	theme	age	686:688	arg1	years					677:681	2 to 3 years	670:681	2 to 3 years of age	670:688	In this pilot study we examine the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age.
27644050	0	36	theme	Mothers	0:6	arg1	Status					17:22	Mothers Secretor Status	0:22	Mothers Secretor Status	0:22	Mothers Secretor Status Affects Development of Childrens Microbiota Composition and Function: A Pilot Study.
27644050	4	37	dep	3	675:675	arg1	to					672:673	to	672:673	to	672:673	In this pilot study we examine the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age.
27644050	2	38	theme	important	390:398	arg1	role					400:403	an important role	387:403	an important role	387:403	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	12	39	dep	CONCLUSIONS	1864:1874	arg1	CONCLUSIONS					1864:1874	CONCLUSIONS Child and mothers' secretor status	1864:1909	CONCLUSIONS Child and mothers' secretor status	1864:1909	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	12	39	dep	CONCLUSIONS	1864:1874	arg1	status					1904:1909	mothers' secretor status	1886:1909	mothers' secretor status	1886:1909	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	12	39	dep	CONCLUSIONS	1864:1874	arg1	Child					1876:1880	Child	1876:1880	Child	1876:1880	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	10	40	theme	life	1534:1537	arg1	months					1524:1529	at least 4 months	1513:1529	at least 4 months of life	1513:1537	In children who were exclusively breast-fed for at least 4 months of life the abundance of the known HMO consumers Bifidobacterium were increased in the children of secretor mothers compared to non-secretor mothers.
27644050	6	41	theme	hemagglutination	818:833	arg1	inhibition					835:844	hemagglutination inhibition	818:844	hemagglutination inhibition technique	818:854	Secretor status was determined from blood and saliva samples using hemagglutination inhibition technique and faecal microbiota composition was examined by 16S rRNA gene sequencing.
27644050	10	42	dep	increased	1601:1609	arg1	compared					1647:1654	compared	1647:1654	compared to non-secretor mothers	1647:1678	In children who were exclusively breast-fed for at least 4 months of life the abundance of the known HMO consumers Bifidobacterium were increased in the children of secretor mothers compared to non-secretor mothers.
27644050	9	43	theme	mothers	1456:1462	arg1	children					1435:1442	children	1435:1442	children of secretor mothers	1435:1462	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	43	theme	mothers	1456:1462	arg1	children					1422:1429	secretor children	1413:1429	secretor children	1413:1429	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	3	44	contain	have	492:495	arg2	quantities					525:534	quantities	525:534	quantities	525:534	However, mothers with inactive secretor genes have altered HMO composition and quantities in their breast milk.
27644050	3	44	contain	have	492:495	arg1	mothers					455:461	mothers	455:461	mothers with inactive secretor genes	455:490	However, mothers with inactive secretor genes have altered HMO composition and quantities in their breast milk.
27644050	3	44	contain	have	492:495	arg2	composition					509:519	altered HMO composition	497:519	altered HMO composition	497:519	However, mothers with inactive secretor genes have altered HMO composition and quantities in their breast milk.
27644050	2	45	theme	breast	318:323	arg1	source					281:286	the optimal source	269:286	the optimal source of nutrition in early life	269:313	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	2	45	theme	breast	318:323	arg1	milk					325:328	breast milk	318:328	breast milk	318:328	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	0	46	theme	Childrens	47:55	arg1	Composition					68:78	Childrens Microbiota Composition	47:78	Childrens Microbiota Composition	47:78	Mothers Secretor Status Affects Development of Childrens Microbiota Composition and Function: A Pilot Study.
27644050	8	47	theme	mothers	1049:1055	arg1	Eleven					1032:1037	Eleven	1032:1037	Eleven	1032:1037	Eleven of the 17 mothers were secretors (S, 64.7%).
27644050	8	47	theme	mothers	1049:1055	arg1	mothers					1049:1055	the 17 mothers	1042:1055	the 17 mothers	1042:1055	Eleven of the 17 mothers were secretors (S, 64.7%).
27644050	7	48	dep	S	1021:1021	arg1	%					1028:1028	71.4%	1024:1028	71.4%	1024:1028	RESULTS Secretor status was determined for 28 eligible children with 20 being secretors (S, 71.4%).
27644050	3	49	theme	secretor	477:484	arg1	genes					486:490	inactive secretor genes	468:490	inactive secretor genes	468:490	However, mothers with inactive secretor genes have altered HMO composition and quantities in their breast milk.
27644050	9	50	theme	R2	1283:1284	arg1	months					1275:1280	at least 4 months	1264:1280	at least 4 months (R2 = 0.167, p = 0.028)	1264:1304	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	50	theme	R2	1283:1284	arg1	0.167					1288:1292	R2 = 0.167	1283:1292	R2 = 0.167	1283:1292	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	10	51	theme	HMO	1566:1568	arg1	consumers					1570:1578	the known HMO consumers	1556:1578	the known HMO consumers	1556:1578	In children who were exclusively breast-fed for at least 4 months of life the abundance of the known HMO consumers Bifidobacterium were increased in the children of secretor mothers compared to non-secretor mothers.
27644050	9	52	theme	UniFrac	1095:1101	arg1	distances					1103:1111	Unweighted UniFrac distances	1084:1111	Unweighted UniFrac distances	1084:1111	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	2	53	theme	optimal	273:279	arg1	milk					325:328	breast milk	318:328	breast milk	318:328	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	2	53	theme	optimal	273:279	arg1	source					281:286	the optimal source	269:286	the optimal source of nutrition in early life	269:313	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	9	54	theme	p	1295:1295	arg1	0.028					1299:1303	p = 0.028	1295:1303	p = 0.028	1295:1303	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	6	55	theme	faecal	860:865	arg1	composition					878:888	faecal microbiota composition	860:888	faecal microbiota composition	860:888	Secretor status was determined from blood and saliva samples using hemagglutination inhibition technique and faecal microbiota composition was examined by 16S rRNA gene sequencing.
27644050	9	56	dep	0.069	1176:1180	arg1	p					1183:1183	p = 0.030	1183:1191	p = 0.030	1183:1191	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	11	57	theme	OTU	1710:1712	arg1	abundance					1694:1702	The relative abundance	1681:1702	The relative abundance of an OTU related to Bacteroides plebeius, a bacterium noted for its capacity to utilise sulphated polysaccharides for growth,	1681:1829	The relative abundance of an OTU related to Bacteroides plebeius, a bacterium noted for its capacity to utilise sulphated polysaccharides for growth, was decreased in these children.
27644050	4	58	theme	secretor	606:613	arg1	status					615:620	secretor status	606:620	secretor status	606:620	In this pilot study we examine the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age.
27644050	10	59	theme	mothers	1639:1645	arg1	children					1618:1625	the children	1614:1625	the children of secretor mothers	1614:1645	In children who were exclusively breast-fed for at least 4 months of life the abundance of the known HMO consumers Bifidobacterium were increased in the children of secretor mothers compared to non-secretor mothers.
27644050	9	60	from	status	1219:1224	arg1	children					1229:1236	children	1229:1236	children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028)	1229:1304	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	12	61	from	impact	1919:1924	arg1	composition					1951:1961	childrens' microbiota composition	1929:1961	childrens' microbiota composition	1929:1961	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	9	62	theme	child	1148:1152	arg1	0.069					1176:1180	R2 = 0.069	1171:1180	R2 = 0.069	1171:1180	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	62	theme	child	1148:1152	arg1	status					1163:1168	child secretor status	1148:1168	child secretor status (R2 = 0.069, p = 0.030)	1148:1192	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	4	63	from	influence	593:601	arg1	composition					655:665	microbiota composition	644:665	microbiota composition at 2 to 3 years of age	644:688	In this pilot study we examine the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age.
27644050	10	64	theme	secretor	1630:1637	arg1	mothers					1639:1645	secretor mothers	1630:1645	secretor mothers	1630:1645	In children who were exclusively breast-fed for at least 4 months of life the abundance of the known HMO consumers Bifidobacterium were increased in the children of secretor mothers compared to non-secretor mothers.
27644050	12	65	theme	microbiota	1940:1949	arg1	composition					1951:1961	childrens' microbiota composition	1929:1961	childrens' microbiota composition	1929:1961	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	6	66	theme	gene	915:918	arg1	sequencing					920:929	16S rRNA gene sequencing	906:929	16S rRNA gene sequencing	906:929	Secretor status was determined from blood and saliva samples using hemagglutination inhibition technique and faecal microbiota composition was examined by 16S rRNA gene sequencing.
27644050	9	67	theme	R2	1171:1172	arg1	0.069					1176:1180	R2 = 0.069	1171:1180	R2 = 0.069	1171:1180	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	67	theme	R2	1171:1172	arg1	status					1163:1168	child secretor status	1148:1168	child secretor status (R2 = 0.069, p = 0.030)	1148:1192	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	3	68	theme	breast	545:550	arg1	milk					552:555	their breast milk	539:555	their breast milk	539:555	However, mothers with inactive secretor genes have altered HMO composition and quantities in their breast milk.
27644050	1	69	theme	BACKGROUND	109:118	arg1	mechanism					124:132	BACKGROUND One mechanism	109:132	BACKGROUND One mechanism by which early life environment may influence long term health	109:195	BACKGROUND One mechanism by which early life environment may influence long term health is through modulation of the gut microbiota.
27644050	6	70	theme	16S	906:908	arg1	sequencing					920:929	16S rRNA gene sequencing	906:929	16S rRNA gene sequencing	906:929	Secretor status was determined from blood and saliva samples using hemagglutination inhibition technique and faecal microbiota composition was examined by 16S rRNA gene sequencing.
27644050	7	71	theme	eligible	978:985	arg1	children					987:994	28 eligible children	975:994	28 eligible children with 20 being secretors (S, 71.4%)	975:1029	RESULTS Secretor status was determined for 28 eligible children with 20 being secretors (S, 71.4%).
27644050	9	72	theme	microbes	1358:1365	arg1	presence/absence					1338:1353	the presence/absence	1334:1353	the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers	1334:1462	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	73	dep	0.167	1288:1292	arg1	0.028					1299:1303	p = 0.028	1295:1303	p = 0.028	1295:1303	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	12	74	theme	secretor	1895:1902	arg1	CONCLUSIONS					1864:1874	CONCLUSIONS Child and mothers' secretor status	1864:1909	CONCLUSIONS Child and mothers' secretor status	1864:1909	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	12	74	theme	secretor	1895:1902	arg1	status					1904:1909	mothers' secretor status	1886:1909	mothers' secretor status	1886:1909	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	11	75	theme	Bacteroides	1725:1735	arg1	plebeius					1737:1744	Bacteroides plebeius	1725:1744	Bacteroides plebeius	1725:1744	The relative abundance of an OTU related to Bacteroides plebeius, a bacterium noted for its capacity to utilise sulphated polysaccharides for growth, was decreased in these children.
27644050	1	76	theme	life	149:152	arg1	environment					154:164	early life environment	143:164	early life environment	143:164	BACKGROUND One mechanism by which early life environment may influence long term health is through modulation of the gut microbiota.
27644050	2	77	from	source	281:286	arg1	life					310:313	early life	304:313	early life	304:313	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	0	78	theme	Pilot	96:100	arg1	Study					102:106	A Pilot Study	94:106	A Pilot Study	94:106	Mothers Secretor Status Affects Development of Childrens Microbiota Composition and Function: A Pilot Study.
27644050	7	79	theme	Secretor	940:947	arg1	status					949:954	RESULTS Secretor status	932:954	RESULTS Secretor status	932:954	RESULTS Secretor status was determined for 28 eligible children with 20 being secretors (S, 71.4%).
27644050	7	80	with	children	987:994	arg1	secretors					1010:1018	20 being secretors	1001:1018	20 being secretors (S, 71.4%)	1001:1029	RESULTS Secretor status was determined for 28 eligible children with 20 being secretors (S, 71.4%).
27644050	7	80	with	children	987:994	arg1	S					1021:1021	S	1021:1021	S	1021:1021	RESULTS Secretor status was determined for 28 eligible children with 20 being secretors (S, 71.4%).
27644050	9	81	theme	mother	1203:1208	arg1	status					1219:1224	mother secretor status	1203:1224	mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028)	1203:1304	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	0	82	theme	Secretor	8:15	arg1	Status					17:22	Mothers Secretor Status	0:22	Mothers Secretor Status	0:22	Mothers Secretor Status Affects Development of Childrens Microbiota Composition and Function: A Pilot Study.
27644050	1	83	theme	gut	226:228	arg1	microbiota					230:239	the gut microbiota	222:239	the gut microbiota	222:239	BACKGROUND One mechanism by which early life environment may influence long term health is through modulation of the gut microbiota.
27644050	8	84	dep	secretors	1062:1070	arg1	S					1073:1073	S	1073:1073	S	1073:1073	Eleven of the 17 mothers were secretors (S, 64.7%).
27644050	8	84	dep	secretors	1062:1070	arg1	%					1080:1080	64.7%	1076:1080	64.7%	1076:1080	Eleven of the 17 mothers were secretors (S, 64.7%).
27644050	2	85	theme	Milk	342:345	arg1	HMOs					365:368	HMOs	365:368	HMOs	365:368	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	2	85	theme	Milk	342:345	arg1	Oligosaccharides					347:362	Human Milk Oligosaccharides	336:362	Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota	336:443	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	2	86	theme	Human	336:340	arg1	HMOs					365:368	HMOs	365:368	HMOs	365:368	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	2	86	theme	Human	336:340	arg1	Oligosaccharides					347:362	Human Milk Oligosaccharides	336:362	Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota	336:443	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	5	87	theme	eligible	718:725	arg1	mothers					727:733	17 eligible mothers	715:733	17 eligible mothers	715:733	METHODS 37 children and 17 eligible mothers were recruited.
27644050	9	88	theme	secretor	1447:1454	arg1	mothers					1456:1462	secretor mothers	1447:1462	secretor mothers	1447:1462	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	2	89	theme	developing	423:432	arg1	microbiota					434:443	the developing microbiota	419:443	the developing microbiota	419:443	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	1	90	dep	term	185:188	arg1	long					180:183	long	180:183	long	180:183	BACKGROUND One mechanism by which early life environment may influence long term health is through modulation of the gut microbiota.
27644050	0	91	theme	Microbiota	57:66	arg1	Composition					68:78	Childrens Microbiota Composition	47:78	Childrens Microbiota Composition	47:78	Mothers Secretor Status Affects Development of Childrens Microbiota Composition and Function: A Pilot Study.
27644050	6	92	theme	saliva	797:802	arg1	samples					804:810	saliva samples	797:810	saliva samples	797:810	Secretor status was determined from blood and saliva samples using hemagglutination inhibition technique and faecal microbiota composition was examined by 16S rRNA gene sequencing.
27644050	11	93	theme	related	1714:1720	arg1	OTU					1710:1712	an OTU	1707:1712	an OTU related to Bacteroides plebeius	1707:1744	The relative abundance of an OTU related to Bacteroides plebeius, a bacterium noted for its capacity to utilise sulphated polysaccharides for growth, was decreased in these children.
27644050	11	93	theme	related	1714:1720	arg1	bacterium					1749:1757	a bacterium	1747:1757	a bacterium noted for its capacity to utilise sulphated polysaccharides for growth	1747:1828	The relative abundance of an OTU related to Bacteroides plebeius, a bacterium noted for its capacity to utilise sulphated polysaccharides for growth, was decreased in these children.
27644050	4	94	from	years	677:681	arg1	composition					655:665	microbiota composition	644:665	microbiota composition at 2 to 3 years of age	644:688	In this pilot study we examine the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age.
27644050	2	95	with	milk	325:328	arg1	HMOs					365:368	HMOs	365:368	HMOs	365:368	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	2	95	with	milk	325:328	arg1	Oligosaccharides					347:362	Human Milk Oligosaccharides	336:362	Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota	336:443	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	0	96	dep	Affects	24:30	arg1	Study					102:106	A Pilot Study	94:106	A Pilot Study	94:106	Mothers Secretor Status Affects Development of Childrens Microbiota Composition and Function: A Pilot Study.
27644050	9	97	from	influence	1321:1329	arg1	presence/absence					1338:1353	the presence/absence	1334:1353	the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers	1334:1462	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	4	98	theme	pilot	566:570	arg1	study					572:576	this pilot study	561:576	this pilot study	561:576	In this pilot study we examine the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age.
27644050	12	99	theme	age	1982:1984	arg1	years					1973:1977	2 to 3 years	1966:1977	2 to 3 years of age	1966:1984	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	2	100	theme	early	304:308	arg1	life					310:313	early life	304:313	early life	304:313	It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota.
27644050	12	101	dep	3	1971:1971	arg1	to					1968:1969	to	1968:1969	to	1968:1969	CONCLUSIONS Child and mothers' secretor status have an impact on childrens' microbiota composition at 2 to 3 years of age.
27644050	1	102	theme	microbiota	230:239	arg1	modulation					208:217	modulation	208:217	modulation of the gut microbiota	208:239	BACKGROUND One mechanism by which early life environment may influence long term health is through modulation of the gut microbiota.
27644050	10	103	theme	consumers	1570:1578	arg1	abundance					1543:1551	the abundance	1539:1551	the abundance of the known HMO consumers Bifidobacterium	1539:1594	In children who were exclusively breast-fed for at least 4 months of life the abundance of the known HMO consumers Bifidobacterium were increased in the children of secretor mothers compared to non-secretor mothers.
27644050	9	104	theme	=	1286:1286	arg1	months					1275:1280	at least 4 months	1264:1280	at least 4 months (R2 = 0.167, p = 0.028)	1264:1304	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
27644050	9	104	theme	=	1286:1286	arg1	0.167					1288:1292	R2 = 0.167	1283:1292	R2 = 0.167	1283:1292	Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers.
26256153	0	0	theme	high	97:100	arg1	weight					112:117	high molecular weight	97:117	high molecular weight	97:117	Fine microstructure of processed chitosan nanofibril networks preserving directional packing and high molecular weight.
26256153	4	1	theme	reflection	689:698	arg1	mode					700:703	reflection mode	689:703	reflection mode	689:703	The comparison of WAXS data in transmission and reflection mode revealed the preferential orientation of the CHI crystals when subjected to mechanical compression constrains.
26256153	6	2	theme	CHI	1138:1140	arg1	processing					1161:1170	CHI nanofibril network processing	1138:1170	CHI nanofibril network processing	1138:1170	The evolution of the CHI allomorphic composition with temperature was studied to further elucidate the mechanism of structural transitions occurring during CHI nanofibril network processing.
26256153	5	3	theme	principal	931:939	arg1	axis					941:944	the principal axis	927:944	the principal axis parallel to the polymer chain axis	927:979	The results are in agreement with the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis.
26256153	5	4	theme	polymer	962:968	arg1	axis					976:979	the polymer chain axis	958:979	the polymer chain axis	958:979	The results are in agreement with the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis.
26256153	5	5	contain	containing	891:900	arg1	nanostructure					877:889	a network nanostructure	867:889	a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis	867:979	The results are in agreement with the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis.
26256153	5	5	contain	containing	891:900	arg2	crystals					913:920	fiber-like crystals	902:920	fiber-like crystals	902:920	The results are in agreement with the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis.
26256153	2	6	theme	mild	313:316	arg1	hydrolysis					318:327	mild hydrolysis	313:327	mild hydrolysis of chitosan (CHI)	313:345	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	3	7	theme	nanofibrils	564:574	arg1	network					541:547	a network	539:547	a network	539:547	Hydrolysis of chitosan yielded a network of crystalline nanofibrils, containing both allomorphs of chitosan: hydrated and anhydrous.
26256153	6	8	theme	allomorphic	1007:1017	arg1	composition					1019:1029	the CHI allomorphic composition	999:1029	the CHI allomorphic composition	999:1029	The evolution of the CHI allomorphic composition with temperature was studied to further elucidate the mechanism of structural transitions occurring during CHI nanofibril network processing.
26256153	0	9	theme	molecular	102:110	arg1	weight					112:117	high molecular weight	97:117	high molecular weight	97:117	Fine microstructure of processed chitosan nanofibril networks preserving directional packing and high molecular weight.
26256153	6	10	theme	CHI	1003:1005	arg1	composition					1019:1029	the CHI allomorphic composition	999:1029	the CHI allomorphic composition	999:1029	The evolution of the CHI allomorphic composition with temperature was studied to further elucidate the mechanism of structural transitions occurring during CHI nanofibril network processing.
26256153	5	11	theme	network	869:875	arg1	nanostructure					877:889	a network nanostructure	867:889	a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis	867:979	The results are in agreement with the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis.
26256153	4	12	theme	preferential	718:729	arg1	orientation					731:741	the preferential orientation	714:741	the preferential orientation of the CHI crystals	714:761	The comparison of WAXS data in transmission and reflection mode revealed the preferential orientation of the CHI crystals when subjected to mechanical compression constrains.
26256153	5	13	theme	nanostructure	877:889	arg1	existence					854:862	the existence	850:862	the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis	850:979	The results are in agreement with the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis.
26256153	4	14	theme	compression	792:802	arg1	constrains					804:813	mechanical compression constrains	781:813	mechanical compression constrains	781:813	The comparison of WAXS data in transmission and reflection mode revealed the preferential orientation of the CHI crystals when subjected to mechanical compression constrains.
26256153	1	15	theme	native	191:196	arg1	packing					209:215	the native structural packing	187:215	the native structural packing	187:215	Crystalline chitosan nanofibril networks were prepared, preserving the native structural packing and the polymer high molecular weight.
26256153	2	16	theme	electron	456:463	arg1	microscopy					465:474	transmission electron microscopy	443:474	transmission electron microscopy (TEM)	443:480	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	2	16	theme	electron	456:463	arg1	TEM					477:479	TEM	477:479	TEM	477:479	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	4	17	theme	mechanical	781:790	arg1	constrains					804:813	mechanical compression constrains	781:813	mechanical compression constrains	781:813	The comparison of WAXS data in transmission and reflection mode revealed the preferential orientation of the CHI crystals when subjected to mechanical compression constrains.
26256153	1	18	theme	structural	198:207	arg1	packing					209:215	the native structural packing	187:215	the native structural packing	187:215	Crystalline chitosan nanofibril networks were prepared, preserving the native structural packing and the polymer high molecular weight.
26256153	2	19	theme	transmission	443:454	arg1	microscopy					465:474	transmission electron microscopy	443:474	transmission electron microscopy (TEM)	443:480	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	2	19	theme	transmission	443:454	arg1	TEM					477:479	TEM	477:479	TEM	477:479	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	4	20	theme	WAXS	659:662	arg1	data					664:667	WAXS data	659:667	WAXS data	659:667	The comparison of WAXS data in transmission and reflection mode revealed the preferential orientation of the CHI crystals when subjected to mechanical compression constrains.
26256153	4	21	theme	CHI	750:752	arg1	crystals					754:761	the CHI crystals	746:761	the CHI crystals	746:761	The comparison of WAXS data in transmission and reflection mode revealed the preferential orientation of the CHI crystals when subjected to mechanical compression constrains.
26256153	6	22	theme	structural	1098:1107	arg1	transitions					1109:1119	structural transitions	1098:1119	structural transitions occurring during CHI nanofibril network processing	1098:1170	The evolution of the CHI allomorphic composition with temperature was studied to further elucidate the mechanism of structural transitions occurring during CHI nanofibril network processing.
26256153	0	23	theme	Fine	0:3	arg1	microstructure					5:18	Fine microstructure	0:18	Fine microstructure of processed chitosan nanofibril networks	0:60	Fine microstructure of processed chitosan nanofibril networks preserving directional packing and high molecular weight.
26256153	4	24	theme	data	664:667	arg1	comparison					645:654	The comparison	641:654	The comparison of WAXS data in transmission and reflection mode	641:703	The comparison of WAXS data in transmission and reflection mode revealed the preferential orientation of the CHI crystals when subjected to mechanical compression constrains.
26256153	2	25	theme	chitosan	332:339	arg1	hydrolysis					318:327	mild hydrolysis	313:327	mild hydrolysis of chitosan (CHI)	313:345	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	2	26	theme	small-	387:392	arg1	scattering					415:424	synchrotron small- and wide-angle X-ray scattering	375:424	synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS)	375:440	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	0	27	theme	processed	23:31	arg1	networks					53:60	processed chitosan nanofibril networks	23:60	processed chitosan nanofibril networks	23:60	Fine microstructure of processed chitosan nanofibril networks preserving directional packing and high molecular weight.
26256153	6	28	theme	transitions	1109:1119	arg1	mechanism					1085:1093	the mechanism	1081:1093	the mechanism of structural transitions occurring during CHI nanofibril network processing	1081:1170	The evolution of the CHI allomorphic composition with temperature was studied to further elucidate the mechanism of structural transitions occurring during CHI nanofibril network processing.
26256153	3	29	theme	crystalline	552:562	arg1	nanofibrils					564:574	crystalline nanofibrils	552:574	crystalline nanofibrils	552:574	Hydrolysis of chitosan yielded a network of crystalline nanofibrils, containing both allomorphs of chitosan: hydrated and anhydrous.
26256153	4	30	from	comparison	645:654	arg1	mode					700:703	reflection mode	689:703	reflection mode	689:703	The comparison of WAXS data in transmission and reflection mode revealed the preferential orientation of the CHI crystals when subjected to mechanical compression constrains.
26256153	4	30	from	comparison	645:654	arg1	transmission					672:683	transmission	672:683	transmission	672:683	The comparison of WAXS data in transmission and reflection mode revealed the preferential orientation of the CHI crystals when subjected to mechanical compression constrains.
26256153	5	31	theme	chain	970:974	arg1	axis					976:979	the polymer chain axis	958:979	the polymer chain axis	958:979	The results are in agreement with the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis.
26256153	3	32	contain	containing	577:586	arg1	network					541:547	a network	539:547	a network	539:547	Hydrolysis of chitosan yielded a network of crystalline nanofibrils, containing both allomorphs of chitosan: hydrated and anhydrous.
26256153	3	32	contain	containing	577:586	arg2	allomorphs					593:602	both allomorphs	588:602	both allomorphs of chitosan: hydrated and anhydrous	588:638	Hydrolysis of chitosan yielded a network of crystalline nanofibrils, containing both allomorphs of chitosan: hydrated and anhydrous.
26256153	1	33	theme	Crystalline	120:130	arg1	networks					152:159	Crystalline chitosan nanofibril networks	120:159	Crystalline chitosan nanofibril networks	120:159	Crystalline chitosan nanofibril networks were prepared, preserving the native structural packing and the polymer high molecular weight.
26256153	1	34	theme	polymer	225:231	arg1	weight					248:253	the polymer high molecular weight	221:253	the polymer high molecular weight	221:253	Crystalline chitosan nanofibril networks were prepared, preserving the native structural packing and the polymer high molecular weight.
26256153	0	35	theme	nanofibril	42:51	arg1	networks					53:60	processed chitosan nanofibril networks	23:60	processed chitosan nanofibril networks	23:60	Fine microstructure of processed chitosan nanofibril networks preserving directional packing and high molecular weight.
26256153	5	36	theme	parallel	946:953	arg1	axis					941:944	the principal axis	927:944	the principal axis parallel to the polymer chain axis	927:979	The results are in agreement with the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis.
26256153	2	37	theme	fine	260:263	arg1	microstructure					265:278	The fine microstructure	256:278	The fine microstructure	256:278	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	5	38	theme	fiber-like	902:911	arg1	crystals					913:920	fiber-like crystals	902:920	fiber-like crystals	902:920	The results are in agreement with the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis.
26256153	1	39	theme	high	233:236	arg1	weight					248:253	the polymer high molecular weight	221:253	the polymer high molecular weight	221:253	Crystalline chitosan nanofibril networks were prepared, preserving the native structural packing and the polymer high molecular weight.
26256153	2	40	theme	X-ray	409:413	arg1	scattering					415:424	synchrotron small- and wide-angle X-ray scattering	375:424	synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS)	375:440	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	0	41	theme	chitosan	33:40	arg1	networks					53:60	processed chitosan nanofibril networks	23:60	processed chitosan nanofibril networks	23:60	Fine microstructure of processed chitosan nanofibril networks preserving directional packing and high molecular weight.
26256153	6	42	theme	composition	1019:1029	arg1	evolution					986:994	The evolution	982:994	The evolution of the CHI allomorphic composition with temperature	982:1046	The evolution of the CHI allomorphic composition with temperature was studied to further elucidate the mechanism of structural transitions occurring during CHI nanofibril network processing.
26256153	2	43	dep	scattering	415:424	arg1	WAXS					436:439	WAXS	436:439	WAXS	436:439	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	2	43	dep	scattering	415:424	arg1	SAXS					427:430	SAXS	427:430	SAXS	427:430	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	1	44	theme	molecular	238:246	arg1	weight					248:253	the polymer high molecular weight	221:253	the polymer high molecular weight	221:253	Crystalline chitosan nanofibril networks were prepared, preserving the native structural packing and the polymer high molecular weight.
26256153	3	45	dep	allomorphs	593:602	arg1	hydrated					617:624	hydrated	617:624	hydrated	617:624	Hydrolysis of chitosan yielded a network of crystalline nanofibrils, containing both allomorphs of chitosan: hydrated and anhydrous.
26256153	3	45	dep	allomorphs	593:602	arg1	anhydrous					630:638	anhydrous	630:638	anhydrous	630:638	Hydrolysis of chitosan yielded a network of crystalline nanofibrils, containing both allomorphs of chitosan: hydrated and anhydrous.
26256153	0	46	theme	directional	73:83	arg1	packing					85:91	directional packing	73:91	directional packing	73:91	Fine microstructure of processed chitosan nanofibril networks preserving directional packing and high molecular weight.
26256153	6	47	theme	network	1153:1159	arg1	processing					1161:1170	CHI nanofibril network processing	1138:1170	CHI nanofibril network processing	1138:1170	The evolution of the CHI allomorphic composition with temperature was studied to further elucidate the mechanism of structural transitions occurring during CHI nanofibril network processing.
26256153	1	48	theme	chitosan	132:139	arg1	networks					152:159	Crystalline chitosan nanofibril networks	120:159	Crystalline chitosan nanofibril networks	120:159	Crystalline chitosan nanofibril networks were prepared, preserving the native structural packing and the polymer high molecular weight.
26256153	0	49	theme	networks	53:60	arg1	microstructure					5:18	Fine microstructure	0:18	Fine microstructure of processed chitosan nanofibril networks	0:60	Fine microstructure of processed chitosan nanofibril networks preserving directional packing and high molecular weight.
26256153	1	50	theme	nanofibril	141:150	arg1	networks					152:159	Crystalline chitosan nanofibril networks	120:159	Crystalline chitosan nanofibril networks	120:159	Crystalline chitosan nanofibril networks were prepared, preserving the native structural packing and the polymer high molecular weight.
26256153	2	51	theme	nanomaterial	287:298	arg1	microstructure					265:278	The fine microstructure	256:278	The fine microstructure	256:278	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	3	52	theme	chitosan	607:614	arg1	allomorphs					593:602	both allomorphs	588:602	both allomorphs of chitosan: hydrated and anhydrous	588:638	Hydrolysis of chitosan yielded a network of crystalline nanofibrils, containing both allomorphs of chitosan: hydrated and anhydrous.
26256153	5	53	with	agreement	835:843	arg1	existence					854:862	the existence	850:862	the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis	850:979	The results are in agreement with the existence of a network nanostructure containing fiber-like crystals with the principal axis parallel to the polymer chain axis.
26256153	6	54	with	evolution	986:994	arg1	temperature					1036:1046	temperature	1036:1046	temperature	1036:1046	The evolution of the CHI allomorphic composition with temperature was studied to further elucidate the mechanism of structural transitions occurring during CHI nanofibril network processing.
26256153	4	55	theme	crystals	754:761	arg1	orientation					731:741	the preferential orientation	714:741	the preferential orientation of the CHI crystals	714:761	The comparison of WAXS data in transmission and reflection mode revealed the preferential orientation of the CHI crystals when subjected to mechanical compression constrains.
26256153	6	56	theme	nanofibril	1142:1151	arg1	processing					1161:1170	CHI nanofibril network processing	1138:1170	CHI nanofibril network processing	1138:1170	The evolution of the CHI allomorphic composition with temperature was studied to further elucidate the mechanism of structural transitions occurring during CHI nanofibril network processing.
26256153	2	57	theme	synchrotron	375:385	arg1	scattering					415:424	synchrotron small- and wide-angle X-ray scattering	375:424	synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS)	375:440	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	2	58	theme	wide-angle	398:407	arg1	scattering					415:424	synchrotron small- and wide-angle X-ray scattering	375:424	synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS)	375:440	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	2	59	theme	electron	486:493	arg1	diffraction					495:505	electron diffraction	486:505	electron diffraction	486:505	The fine microstructure of the nanomaterial, obtained by mild hydrolysis of chitosan (CHI), was characterized by using synchrotron small- and wide-angle X-ray scattering (SAXS and WAXS), transmission electron microscopy (TEM) and electron diffraction.
26256153	3	60	theme	chitosan	522:529	arg1	Hydrolysis					508:517	Hydrolysis	508:517	Hydrolysis of chitosan	508:529	Hydrolysis of chitosan yielded a network of crystalline nanofibrils, containing both allomorphs of chitosan: hydrated and anhydrous.
24443356	2	0	theme	most	312:315	arg1	one					301:303	one	301:303	one	301:303	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	2	0	theme	most	312:315	arg1	most					312:315	most	312:315	most	312:315	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	2	0	theme	most	312:315	arg1	cell					279:282	liver cell	273:282	liver cell	273:282	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	2	0	theme	most	312:315	arg1	cells					351:355	all differentiated cells	332:355	all differentiated cells	332:355	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	6	1	theme	best	952:955	arg1	interactions					971:982	the best cell/scaffold interactions	948:982	the best cell/scaffold interactions for regeneration of liver cells	948:1014	More specifically, the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold presented the best cell/scaffold interactions for regeneration of liver cells.
24443356	6	2	theme	PEDOT	868:872	arg1	blend					847:851	the blend	843:851	the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold	843:936	More specifically, the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold presented the best cell/scaffold interactions for regeneration of liver cells.
24443356	4	3	theme	engineering	554:564	arg1	techniques					566:575	tissue engineering techniques	547:575	tissue engineering techniques	547:575	In this article, using tissue engineering techniques, we grew hepatocyte cells in scaffolds with various compositions.
24443356	6	4	theme	hyaluronan	856:865	arg1	blend					847:851	the blend	843:851	the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold	843:936	More specifically, the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold presented the best cell/scaffold interactions for regeneration of liver cells.
24443356	2	5	theme	membrane	251:258	arg1	potential					260:268	its specific cell membrane potential	233:268	its specific cell membrane potential	233:268	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	4	6	theme	tissue	547:552	arg1	techniques					566:575	tissue engineering techniques	547:575	tissue engineering techniques	547:575	In this article, using tissue engineering techniques, we grew hepatocyte cells in scaffolds with various compositions.
24443356	5	7	theme	poly	708:711	arg1	polymer					697:703	conducting polymer	686:703	conducting polymer of poly (3,4-ethylenedioxythiophene) (PEDOT)	686:748	It was found that the scaffolds containing conducting polymer of poly (3,4-ethylenedioxythiophene) (PEDOT) provide the best condition for attachment and proliferation of the cells.
24443356	6	8	theme	cells	1010:1014	arg1	regeneration					988:999	regeneration	988:999	regeneration of liver cells	988:1014	More specifically, the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold presented the best cell/scaffold interactions for regeneration of liver cells.
24443356	2	9	theme	cell	246:249	arg1	potential					260:268	its specific cell membrane potential	233:268	its specific cell membrane potential	233:268	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	4	10	theme	various	621:627	arg1	compositions					629:640	various compositions	621:640	various compositions	621:640	In this article, using tissue engineering techniques, we grew hepatocyte cells in scaffolds with various compositions.
24443356	2	11	theme	specific	237:244	arg1	potential					260:268	its specific cell membrane potential	233:268	its specific cell membrane potential	233:268	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	1	12	theme	cell	154:157	arg1	potential					115:123	a cell membrane potential	99:123	a cell membrane potential	99:123	It is known that there is a correlation between a cell membrane potential and the proliferation of the cell.
24443356	1	12	theme	cell	154:157	arg1	proliferation					133:145	the proliferation	129:145	the proliferation of the cell	129:157	It is known that there is a correlation between a cell membrane potential and the proliferation of the cell.
24443356	2	13	theme	differentiated	336:349	arg1	cells					351:355	all differentiated cells	332:355	all differentiated cells	332:355	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	6	14	theme	gelatin-chitosan-based	906:927	arg1	scaffold					929:936	gelatin-chitosan-based scaffold	906:936	gelatin-chitosan-based scaffold	906:936	More specifically, the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold presented the best cell/scaffold interactions for regeneration of liver cells.
24443356	0	15	theme	liver	25:29	arg1	engineering					38:48	liver tissue engineering	25:48	liver tissue engineering	25:48	Conducting scaffolds for liver tissue engineering.
24443356	5	16	contain	containing	675:684	arg1	scaffolds					665:673	the scaffolds	661:673	the scaffolds containing conducting polymer of poly (3,4-ethylenedioxythiophene) (PEDOT)	661:748	It was found that the scaffolds containing conducting polymer of poly (3,4-ethylenedioxythiophene) (PEDOT) provide the best condition for attachment and proliferation of the cells.
24443356	5	16	contain	containing	675:684	arg2	polymer					697:703	conducting polymer	686:703	conducting polymer of poly (3,4-ethylenedioxythiophene) (PEDOT)	686:748	It was found that the scaffolds containing conducting polymer of poly (3,4-ethylenedioxythiophene) (PEDOT) provide the best condition for attachment and proliferation of the cells.
24443356	6	17	from	dopants	895:901	arg1	scaffold					929:936	gelatin-chitosan-based scaffold	906:936	gelatin-chitosan-based scaffold	906:936	More specifically, the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold presented the best cell/scaffold interactions for regeneration of liver cells.
24443356	2	18	theme	liver	273:277	arg1	one					301:303	one	301:303	one	301:303	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	2	18	theme	liver	273:277	arg1	most					312:315	most	312:315	most	312:315	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	2	18	theme	liver	273:277	arg1	cell					279:282	liver cell	273:282	liver cell	273:282	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	3	19	theme	liver	424:428	arg1	cells					430:434	liver cells	424:434	liver cells	424:434	We hypothesized that this phenomenon can be emphasized by growing liver cells in conducting scaffolds that can increase the electrical communication among the cells.
24443356	4	20	theme	hepatocyte	586:595	arg1	cells					597:601	hepatocyte cells	586:601	hepatocyte cells	586:601	In this article, using tissue engineering techniques, we grew hepatocyte cells in scaffolds with various compositions.
24443356	6	21	theme	liver	1004:1008	arg1	cells					1010:1014	liver cells	1004:1014	liver cells	1004:1014	More specifically, the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold presented the best cell/scaffold interactions for regeneration of liver cells.
24443356	0	22	theme	tissue	31:36	arg1	engineering					38:48	liver tissue engineering	25:48	liver tissue engineering	25:48	Conducting scaffolds for liver tissue engineering.
24443356	5	23	theme	conducting	686:695	arg1	polymer					697:703	conducting polymer	686:703	conducting polymer of poly (3,4-ethylenedioxythiophene) (PEDOT)	686:748	It was found that the scaffolds containing conducting polymer of poly (3,4-ethylenedioxythiophene) (PEDOT) provide the best condition for attachment and proliferation of the cells.
24443356	5	24	theme	cells	817:821	arg1	proliferation					796:808	proliferation	796:808	proliferation	796:808	It was found that the scaffolds containing conducting polymer of poly (3,4-ethylenedioxythiophene) (PEDOT) provide the best condition for attachment and proliferation of the cells.
24443356	5	24	theme	cells	817:821	arg1	attachment					781:790	attachment	781:790	attachment	781:790	It was found that the scaffolds containing conducting polymer of poly (3,4-ethylenedioxythiophene) (PEDOT) provide the best condition for attachment and proliferation of the cells.
24443356	2	25	theme	cells	201:205	arg1	capacity					183:190	The high proliferation capacity	160:190	The high proliferation capacity of liver cells	160:205	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	5	26	theme	best	762:765	arg1	condition					767:775	the best condition	758:775	the best condition for attachment and proliferation of the cells	758:821	It was found that the scaffolds containing conducting polymer of poly (3,4-ethylenedioxythiophene) (PEDOT) provide the best condition for attachment and proliferation of the cells.
24443356	4	27	with	scaffolds	606:614	arg1	compositions					629:640	various compositions	621:640	various compositions	621:640	In this article, using tissue engineering techniques, we grew hepatocyte cells in scaffolds with various compositions.
24443356	2	28	theme	liver	195:199	arg1	cells					201:205	liver cells	195:205	liver cells	195:205	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	1	29	theme	cell	101:104	arg1	potential					115:123	a cell membrane potential	99:123	a cell membrane potential	99:123	It is known that there is a correlation between a cell membrane potential and the proliferation of the cell.
24443356	6	30	theme	collagen	879:886	arg1	blend					847:851	the blend	843:851	the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold	843:936	More specifically, the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold presented the best cell/scaffold interactions for regeneration of liver cells.
24443356	1	31	theme	membrane	106:113	arg1	potential					115:123	a cell membrane potential	99:123	a cell membrane potential	99:123	It is known that there is a correlation between a cell membrane potential and the proliferation of the cell.
24443356	2	32	theme	proliferation	169:181	arg1	capacity					183:190	The high proliferation capacity	160:190	The high proliferation capacity of liver cells	160:205	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	3	33	theme	conducting	439:448	arg1	scaffolds					450:458	conducting scaffolds	439:458	conducting scaffolds that can increase the electrical communication among the cells	439:521	We hypothesized that this phenomenon can be emphasized by growing liver cells in conducting scaffolds that can increase the electrical communication among the cells.
24443356	2	34	theme	high	164:167	arg1	capacity					183:190	The high proliferation capacity	160:190	The high proliferation capacity of liver cells	160:205	The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells.
24443356	3	35	theme	electrical	482:491	arg1	communication					493:505	the electrical communication	478:505	the electrical communication among the cells	478:521	We hypothesized that this phenomenon can be emphasized by growing liver cells in conducting scaffolds that can increase the electrical communication among the cells.
24443356	6	36	theme	cell/scaffold	957:969	arg1	interactions					971:982	the best cell/scaffold interactions	948:982	the best cell/scaffold interactions for regeneration of liver cells	948:1014	More specifically, the blend of hyaluronan, PEDOT, and collagen (I) as dopants in gelatin-chitosan-based scaffold presented the best cell/scaffold interactions for regeneration of liver cells.
27920191	2	0	dep	solution	447:454	arg1	3.0					462:464	3.0	462:464	3.0	462:464	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	2	0	dep	solution	447:454	arg1	4.5 mg					471:476	4.5 mg	471:476	4.5 mg	471:476	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	2	0	dep	solution	447:454	arg1	1.5					457:459	1.5	457:459	1.5	457:459	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	11	1	theme	small	1673:1677	arg1	indicators					1700:1709	indicators	1700:1709	indicators of improved gut health	1700:1732	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	11	1	theme	small	1673:1677	arg1	intestine					1679:1687	the small intestine	1669:1687	the small intestine	1669:1687	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	3	2	theme	incubation	571:580	arg1	d 12					563:566	d 12	563:566	d 12 of incubation	563:580	The RFO solution was injected into the air sac on d 12 of incubation.
27920191	10	3	theme	marker	1351:1356	arg1	genes					1358:1362	T cell and B cell marker genes	1333:1362	T cell and B cell marker genes	1333:1362	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	10	3	theme	marker	1351:1356	arg1	chB6					1317:1320	chB6	1317:1320	chB6	1317:1320	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	10	3	theme	marker	1351:1356	arg1	CD3					1309:1311	CD3	1309:1311	CD3	1309:1311	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	1	4	theme	proventriculus	216:229	arg1	weight					206:211	relative weight	197:211	relative weight of proventriculus, gizzard, drumstick and breast muscles	197:268	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	4	theme	proventriculus	216:229	arg1	ileum					275:279	ileum	275:279	ileum	275:279	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	4	theme	proventriculus	216:229	arg1	prebiotic					164:172	a prebiotic	162:172	a prebiotic on growth performance	162:194	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	11	5	contain	has	1588:1590	arg1	RFO					1584:1586	RFO	1584:1586	RFO	1584:1586	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	11	5	contain	has	1588:1590	arg2	potential					1596:1604	the potential	1592:1604	the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health	1592:1732	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	5	6	theme	small	736:740	arg1	intestine					742:750	the small intestine	732:750	the small intestine	732:750	Total RNA was extracted from the small intestine, and RT-qPCR was performed to quantify mRNA levels of marker genes of immune cells.
27920191	2	7	theme	diluents	502:509	arg1	mL					485:486	0.2 mL	481:486	0.2 mL of an aqueous diluents	481:509	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	10	8	theme	B	1344:1344	arg1	cell					1346:1349	B cell	1344:1349	B cell	1344:1349	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	1	9	theme	raffinose	143:151	arg1	injection					130:138	in ovo injection	123:138	in ovo injection of raffinose (RFO)	123:157	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	8	10	dep	increased	1071:1079	arg1	<					1093:1093	P < 0.01	1091:1098	P < 0.01	1091:1098	On hatch d, the villus height increased linearly (P < 0.01) with an increasing dose of RFO.
27920191	0	11	theme	chicken	99:105	arg1	parameters					77:86	gut health parameters	66:86	gut health parameters	66:86	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	0	11	theme	chicken	99:105	arg1	performance					50:60	growth performance	43:60	growth performance	43:60	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	9	12	theme	increasing	1142:1151	arg1	dose					1153:1156	an increasing dose	1139:1156	an increasing dose of RFO	1139:1163	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	2	13	theme	solution	447:454	arg1	shell					421:425	intact shell	414:425	intact shell	414:425	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	2	13	theme	solution	447:454	arg1	levels					433:438	3 levels	431:438	3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents)	431:510	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	8	14	theme	RFO	1128:1130	arg1	dose					1120:1123	an increasing dose	1106:1123	an increasing dose of RFO	1106:1130	On hatch d, the villus height increased linearly (P < 0.01) with an increasing dose of RFO.
27920191	1	15	from	weight	206:211	arg1	performance					184:194	growth performance	177:194	growth performance	177:194	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	9	16	theme	villus	1179:1184	arg1	height					1186:1191	villus height	1179:1191	villus height	1179:1191	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	10	17	theme	CD3	1309:1311	arg1	levels					1299:1304	The expression levels	1284:1304	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively,	1284:1377	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	0	18	theme	growth	43:48	arg1	performance					50:60	growth performance	43:60	growth performance	43:60	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	10	19	theme	dose	1415:1418	arg1	RFO					1420:1422	high dose RFO	1410:1422	high dose RFO (4.5 mg)	1410:1431	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	10	19	theme	dose	1415:1418	arg1	4.5 mg					1425:1430	4.5 mg	1425:1430	4.5 mg	1425:1430	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	1	20	theme	in	123:124	arg1	injection					130:138	in ovo injection	123:138	in ovo injection of raffinose (RFO)	123:157	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	21	theme	mucosa	281:286	arg1	morphology					288:297	mucosa morphology	281:297	mucosa morphology	281:297	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	22	theme	injection	130:138	arg1	effects					112:118	The effects	108:118	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology	108:297	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	0	23	from	Effect	0:5	arg1	parameters					77:86	gut health parameters	66:86	gut health parameters	66:86	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	0	23	from	Effect	0:5	arg1	performance					50:60	growth performance	43:60	growth performance	43:60	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	0	24	theme	health	70:75	arg1	parameters					77:86	gut health parameters	66:86	gut health parameters	66:86	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	9	25	theme	crypt	1211:1215	arg1	ratio					1223:1227	the villus height and villus height:crypt depth ratio	1175:1227	the villus height and villus height:crypt depth ratio (P < 0.05)	1175:1238	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	9	25	theme	crypt	1211:1215	arg1	<					1232:1232	P < 0.05	1230:1237	P < 0.05	1230:1237	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	6	26	contain	had	853:855	arg2	>					882:882	P > 0.05	880:887	P > 0.05	880:887	Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks.
27920191	6	26	contain	had	853:855	arg1	Injection					836:844	Injection	836:844	Injection of RFO	836:851	Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks.
27920191	6	26	contain	had	853:855	arg2	effect					872:877	no significant effect	857:877	no significant effect (P > 0.05)	857:888	Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks.
27920191	4	27	theme	standard	613:620	arg1	diet					622:625	a standard diet	611:625	a standard diet	611:625	In total 144 birds were fed a standard diet and management and sacrificed at d 21 post hatch for collection of samples.
27920191	11	28	theme	RFO	1480:1482	arg1	dose					1472:1475	an increasing dose	1458:1475	an increasing dose of RFO in ovo injection	1458:1499	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	6	29	theme	P	880:880	arg1	effect					872:877	no significant effect	857:877	no significant effect (P > 0.05)	857:888	Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks.
27920191	6	29	theme	P	880:880	arg1	>					882:882	P > 0.05	880:887	P > 0.05	880:887	Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks.
27920191	9	30	theme	P	1230:1230	arg1	ratio					1223:1227	the villus height and villus height:crypt depth ratio	1175:1227	the villus height and villus height:crypt depth ratio (P < 0.05)	1175:1238	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	9	30	theme	P	1230:1230	arg1	<					1232:1232	P < 0.05	1230:1237	P < 0.05	1230:1237	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	11	31	theme	gut	1723:1725	arg1	health					1727:1732	improved gut health	1714:1732	improved gut health	1714:1732	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	8	32	theme	P	1091:1091	arg1	<					1093:1093	P < 0.01	1091:1098	P < 0.01	1091:1098	On hatch d, the villus height increased linearly (P < 0.01) with an increasing dose of RFO.
27920191	2	33	theme	fertilized	350:359	arg1	eggs					361:364	240 fertilized eggs	346:364	240 fertilized eggs	346:364	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	0	34	theme	in	10:11	arg1	injection					17:25	in ovo injection	10:25	in ovo injection of raffinose	10:38	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	11	35	theme	broilers	1574:1581	arg1	performance					1540:1550	growth performance	1533:1550	growth performance	1533:1550	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	11	35	theme	broilers	1574:1581	arg1	yield					1565:1569	slaughter yield	1555:1569	slaughter yield	1555:1569	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	3	36	theme	RFO	517:519	arg1	solution					521:528	The RFO solution	513:528	The RFO solution	513:528	The RFO solution was injected into the air sac on d 12 of incubation.
27920191	11	37	theme	ovo	1487:1489	arg1	injection					1491:1499	ovo injection	1487:1499	ovo injection	1487:1499	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	0	38	theme	injection	17:25	arg1	Effect					0:5	Effect	0:5	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.	0:106	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	6	39	theme	RFO	849:851	arg1	Injection					836:844	Injection	836:844	Injection of RFO	836:851	Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks.
27920191	2	40	dep	fertilized	350:359	arg1	240					346:348	240	346:348	240	346:348	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	1	41	from	ileum	275:279	arg1	performance					184:194	growth performance	177:194	growth performance	177:194	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	42	theme	muscles	262:268	arg1	weight					206:211	relative weight	197:211	relative weight of proventriculus, gizzard, drumstick and breast muscles	197:268	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	42	theme	muscles	262:268	arg1	ileum					275:279	ileum	275:279	ileum	275:279	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	42	theme	muscles	262:268	arg1	prebiotic					164:172	a prebiotic	162:172	a prebiotic on growth performance	162:194	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	11	43	theme	improved	1714:1721	arg1	health					1727:1732	improved gut health	1714:1732	improved gut health	1714:1732	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	11	44	theme	slaughter	1555:1563	arg1	yield					1565:1569	slaughter yield	1555:1569	slaughter yield	1555:1569	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	7	45	dep	proventriculus	958:971	arg1	the					954:956	the	954:956	the	954:956	On d 21, the relative weight of the proventriculus, drumstick, breast, and gizzard was not affected (P > 0.05) by RFO.
27920191	11	46	theme	health	1727:1732	arg1	indicators					1700:1709	indicators	1700:1709	indicators of improved gut health	1700:1732	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	11	46	theme	health	1727:1732	arg1	intestine					1679:1687	the small intestine	1669:1687	the small intestine	1669:1687	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	8	47	theme	increasing	1109:1118	arg1	dose					1120:1123	an increasing dose	1106:1123	an increasing dose of RFO	1106:1130	On hatch d, the villus height increased linearly (P < 0.01) with an increasing dose of RFO.
27920191	10	48	theme	chB6	1317:1320	arg1	levels					1299:1304	The expression levels	1284:1304	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively,	1284:1377	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	5	49	theme	genes	813:817	arg1	levels					796:801	mRNA levels	791:801	mRNA levels of marker genes of immune cells	791:833	Total RNA was extracted from the small intestine, and RT-qPCR was performed to quantify mRNA levels of marker genes of immune cells.
27920191	5	50	theme	immune	822:827	arg1	cells					829:833	immune cells	822:833	immune cells	822:833	Total RNA was extracted from the small intestine, and RT-qPCR was performed to quantify mRNA levels of marker genes of immune cells.
27920191	7	51	theme	drumstick	974:982	arg1	weight					944:949	the relative weight	931:949	the relative weight of the proventriculus, drumstick, breast, and gizzard	931:1003	On d 21, the relative weight of the proventriculus, drumstick, breast, and gizzard was not affected (P > 0.05) by RFO.
27920191	10	52	theme	T	1333:1333	arg1	cell					1335:1338	T cell	1333:1338	T cell	1333:1338	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	1	53	theme	relative	197:204	arg1	weight					206:211	relative weight	197:211	relative weight of proventriculus, gizzard, drumstick and breast muscles	197:268	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	4	54	theme	samples	694:700	arg1	collection					680:689	collection	680:689	collection of samples	680:700	In total 144 birds were fed a standard diet and management and sacrificed at d 21 post hatch for collection of samples.
27920191	7	55	theme	gizzard	997:1003	arg1	weight					944:949	the relative weight	931:949	the relative weight of the proventriculus, drumstick, breast, and gizzard	931:1003	On d 21, the relative weight of the proventriculus, drumstick, breast, and gizzard was not affected (P > 0.05) by RFO.
27920191	3	56	theme	air	552:554	arg1	sac					556:558	the air sac	548:558	the air sac	548:558	The RFO solution was injected into the air sac on d 12 of incubation.
27920191	10	57	theme	cell	1346:1349	arg1	genes					1358:1362	T cell and B cell marker genes	1333:1362	T cell and B cell marker genes	1333:1362	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	10	57	theme	cell	1346:1349	arg1	chB6					1317:1320	chB6	1317:1320	chB6	1317:1320	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	10	57	theme	cell	1346:1349	arg1	CD3					1309:1311	CD3	1309:1311	CD3	1309:1311	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	0	58	theme	raffinose	30:38	arg1	injection					17:25	in ovo injection	10:25	in ovo injection of raffinose	10:38	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	2	59	theme	aqueous	494:500	arg1	diluents					502:509	an aqueous diluents	491:509	an aqueous diluents	491:509	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	5	60	theme	Total	703:707	arg1	RNA					709:711	Total RNA	703:711	Total RNA	703:711	Total RNA was extracted from the small intestine, and RT-qPCR was performed to quantify mRNA levels of marker genes of immune cells.
27920191	9	61	theme	height	1204:1209	arg1	ratio					1223:1227	the villus height and villus height:crypt depth ratio	1175:1227	the villus height and villus height:crypt depth ratio (P < 0.05)	1175:1238	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	9	61	theme	height	1204:1209	arg1	<					1232:1232	P < 0.05	1230:1237	P < 0.05	1230:1237	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	10	62	theme	high	1410:1413	arg1	RFO					1420:1422	high dose RFO	1410:1422	high dose RFO (4.5 mg)	1410:1431	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	10	62	theme	high	1410:1413	arg1	4.5 mg					1425:1430	4.5 mg	1425:1430	4.5 mg	1425:1430	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	2	63	theme	RFO	443:445	arg1	solution					447:454	RFO solution	443:454	RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents)	443:510	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	1	64	from	prebiotic	164:172	arg1	performance					184:194	growth performance	177:194	growth performance	177:194	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	9	65	theme	RFO	1161:1163	arg1	dose					1153:1156	an increasing dose	1139:1156	an increasing dose of RFO	1139:1163	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	11	66	from	dose	1472:1475	arg1	injection					1491:1499	ovo injection	1487:1499	ovo injection	1487:1499	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	11	67	from	immunity	1657:1664	arg1	indicators					1700:1709	indicators	1700:1709	indicators of improved gut health	1700:1732	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	11	67	from	immunity	1657:1664	arg1	intestine					1679:1687	the small intestine	1669:1687	the small intestine	1669:1687	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	7	68	theme	breast	985:990	arg1	weight					944:949	the relative weight	931:949	the relative weight of the proventriculus, drumstick, breast, and gizzard	931:1003	On d 21, the relative weight of the proventriculus, drumstick, breast, and gizzard was not affected (P > 0.05) by RFO.
27920191	9	69	theme	height	1186:1191	arg1	ratio					1223:1227	the villus height and villus height:crypt depth ratio	1175:1227	the villus height and villus height:crypt depth ratio (P < 0.05)	1175:1238	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	9	69	theme	height	1186:1191	arg1	<					1232:1232	P < 0.05	1230:1237	P < 0.05	1230:1237	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	2	70	theme	intact	414:419	arg1	shell					421:425	intact shell	414:425	intact shell	414:425	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	0	71	theme	gut	66:68	arg1	parameters					77:86	gut health parameters	66:86	gut health parameters	66:86	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	2	72	dep	groups	386:391	arg1	non-injected					396:407	non-injected	396:407	non-injected	396:407	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	9	73	theme	villus	1197:1202	arg1	height					1204:1209	villus height	1197:1209	villus height	1197:1209	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	8	74	theme	hatch	1044:1048	arg1	d					1050:1050	hatch d	1044:1050	hatch d	1044:1050	On hatch d, the villus height increased linearly (P < 0.01) with an increasing dose of RFO.
27920191	7	75	theme	proventriculus	958:971	arg1	weight					944:949	the relative weight	931:949	the relative weight of the proventriculus, drumstick, breast, and gizzard	931:1003	On d 21, the relative weight of the proventriculus, drumstick, breast, and gizzard was not affected (P > 0.05) by RFO.
27920191	0	76	dep	in	10:11	arg1	ovo					13:15	ovo	13:15	ovo	13:15	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	2	77	with	non-injected	396:407	arg1	shell					421:425	intact shell	414:425	intact shell	414:425	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	2	77	with	non-injected	396:407	arg1	levels					433:438	3 levels	431:438	3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents)	431:510	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	7	78	dep	affected	1013:1020	arg1	>					1025:1025	P > 0.05	1023:1030	P > 0.05	1023:1030	On d 21, the relative weight of the proventriculus, drumstick, breast, and gizzard was not affected (P > 0.05) by RFO.
27920191	6	79	theme	chicks	914:919	arg1	weight					904:909	d one body weight	893:909	d one body weight of chicks	893:919	Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks.
27920191	5	80	theme	mRNA	791:794	arg1	levels					796:801	mRNA levels	791:801	mRNA levels of marker genes of immune cells	791:833	Total RNA was extracted from the small intestine, and RT-qPCR was performed to quantify mRNA levels of marker genes of immune cells.
27920191	0	81	theme	broiler	91:97	arg1	chicken					99:105	broiler chicken	91:105	broiler chicken	91:105	Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken.
27920191	9	82	theme	depth	1217:1221	arg1	ratio					1223:1227	the villus height and villus height:crypt depth ratio	1175:1227	the villus height and villus height:crypt depth ratio (P < 0.05)	1175:1238	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	9	82	theme	depth	1217:1221	arg1	<					1232:1232	P < 0.05	1230:1237	P < 0.05	1230:1237	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	9	83	from	depth	1269:1273	arg1	d 21					1278:1281	d 21	1278:1281	d 21	1278:1281	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	8	84	theme	villus	1057:1062	arg1	height					1064:1069	the villus height	1053:1069	the villus height	1053:1069	On hatch d, the villus height increased linearly (P < 0.01) with an increasing dose of RFO.
27920191	7	85	theme	relative	935:942	arg1	weight					944:949	the relative weight	931:949	the relative weight of the proventriculus, drumstick, breast, and gizzard	931:1003	On d 21, the relative weight of the proventriculus, drumstick, breast, and gizzard was not affected (P > 0.05) by RFO.
27920191	1	86	theme	Cobb	316:319	arg1	broilers					325:332	Cobb 500 broilers	316:332	Cobb 500 broilers	316:332	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	11	87	theme	ileum	1619:1623	arg1	morphology					1632:1641	ileum mucosa morphology	1619:1641	ileum mucosa morphology	1619:1641	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	1	88	theme	gizzard	232:238	arg1	weight					206:211	relative weight	197:211	relative weight of proventriculus, gizzard, drumstick and breast muscles	197:268	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	88	theme	gizzard	232:238	arg1	ileum					275:279	ileum	275:279	ileum	275:279	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	88	theme	gizzard	232:238	arg1	prebiotic					164:172	a prebiotic	162:172	a prebiotic on growth performance	162:194	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	11	89	theme	growth	1533:1538	arg1	performance					1540:1550	growth performance	1533:1550	growth performance	1533:1550	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	6	90	theme	significant	860:870	arg1	effect					872:877	no significant effect	857:877	no significant effect (P > 0.05)	857:888	Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks.
27920191	6	90	theme	significant	860:870	arg1	>					882:882	P > 0.05	880:887	P > 0.05	880:887	Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks.
27920191	2	91	theme	eggs	361:364	arg1	total					337:341	A total	335:341	A total of 240 fertilized eggs	335:364	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	1	92	dep	in	123:124	arg1	ovo					126:128	ovo	126:128	ovo	126:128	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	93	theme	drumstick	241:249	arg1	weight					206:211	relative weight	197:211	relative weight of proventriculus, gizzard, drumstick and breast muscles	197:268	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	93	theme	drumstick	241:249	arg1	ileum					275:279	ileum	275:279	ileum	275:279	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	1	93	theme	drumstick	241:249	arg1	prebiotic					164:172	a prebiotic	162:172	a prebiotic on growth performance	162:194	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	5	94	theme	cells	829:833	arg1	genes					813:817	marker genes	806:817	marker genes of immune cells	806:833	Total RNA was extracted from the small intestine, and RT-qPCR was performed to quantify mRNA levels of marker genes of immune cells.
27920191	10	95	theme	expression	1288:1297	arg1	levels					1299:1304	The expression levels	1284:1304	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively,	1284:1377	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	1	96	theme	breast	255:260	arg1	muscles					262:268	breast muscles	255:268	breast muscles	255:268	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	7	97	theme	P	1023:1023	arg1	>					1025:1025	P > 0.05	1023:1030	P > 0.05	1023:1030	On d 21, the relative weight of the proventriculus, drumstick, breast, and gizzard was not affected (P > 0.05) by RFO.
27920191	11	98	theme	mucosa	1625:1630	arg1	morphology					1632:1641	ileum mucosa morphology	1619:1641	ileum mucosa morphology	1619:1641	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	5	99	theme	marker	806:811	arg1	genes					813:817	marker genes	806:817	marker genes of immune cells	806:833	Total RNA was extracted from the small intestine, and RT-qPCR was performed to quantify mRNA levels of marker genes of immune cells.
27920191	6	100	theme	body	899:902	arg1	weight					904:909	d one body weight	893:909	d one body weight of chicks	893:919	Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks.
27920191	1	101	dep	prebiotic	164:172	arg1	morphology					288:297	mucosa morphology	281:297	mucosa morphology	281:297	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	11	102	theme	increasing	1461:1470	arg1	dose					1472:1475	an increasing dose	1458:1475	an increasing dose of RFO in ovo injection	1458:1499	In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.
27920191	1	103	theme	growth	177:182	arg1	performance					184:194	growth performance	177:194	growth performance	177:194	The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers.
27920191	9	104	theme	crypt	1263:1267	arg1	depth					1269:1273	the crypt depth	1259:1273	the crypt depth on d 21	1259:1281	Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21.
27920191	6	105	theme	d	893:893	arg1	weight					904:909	d one body weight	893:909	d one body weight of chicks	893:919	Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks.
27920191	2	106	from	1.5	457:459	arg1	mL					485:486	0.2 mL	481:486	0.2 mL of an aqueous diluents	481:509	A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents).
27920191	10	107	theme	cell	1335:1338	arg1	genes					1358:1362	T cell and B cell marker genes	1333:1362	T cell and B cell marker genes	1333:1362	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	10	107	theme	cell	1335:1338	arg1	chB6					1317:1320	chB6	1317:1320	chB6	1317:1320	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
27920191	10	107	theme	cell	1335:1338	arg1	CD3					1309:1311	CD3	1309:1311	CD3	1309:1311	The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg).
26759810	4	0	theme	original	1181:1188	arg1	conditions					1190:1199	original conditions	1181:1199	original conditions	1181:1199	These findings indicated the D-ribose production performance was significantly improved compared to that under original conditions.
26759810	3	1	theme	%	547:547	arg1	parameters					491:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	1	theme	%	547:547	arg1	volume					558:563	10% inoculum volume	545:563	10% inoculum volume	545:563	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	2	theme	optimized	750:758	arg1	production					769:778	the optimized D-ribose production	746:778	the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h	746:888	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	3	theme	productivity	863:874	arg1	concentration					801:813	the concentration	797:813	the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h	797:888	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	0	4	theme	Transketolase-Deficient	69:91	arg1	Strain					93:98	a Transketolase-Deficient Strain	67:98	a Transketolase-Deficient Strain	67:98	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
26759810	3	5	theme	yeast	1017:1021	arg1	extract					1023:1029	1 g/L yeast extract	1011:1029	1 g/L yeast extract	1011:1029	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	5	theme	yeast	1017:1021	arg1	compositions					924:935	the medium compositions	913:935	the medium compositions	913:935	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	6	theme	inoculum	549:556	arg1	parameters					491:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	6	theme	inoculum	549:556	arg1	volume					558:563	10% inoculum volume	545:563	10% inoculum volume	545:563	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	2	7	from	material	329:336	arg1	performance					302:312	maximum D-ribose production performance	274:312	maximum D-ribose production performance from cheap raw material corn starch hydrolysate	274:360	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	4	8	theme	D-ribose	1099:1106	arg1	performance					1119:1129	the D-ribose production performance	1095:1129	the D-ribose production performance	1095:1129	These findings indicated the D-ribose production performance was significantly improved compared to that under original conditions.
26759810	0	9	from	Improvement	0:10	arg1	Hydrolysate					52:62	Corn Starch Hydrolysate	40:62	Corn Starch Hydrolysate	40:62	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
26759810	1	10	used	used	173:176	arg2	D-Ribose					127:134	D-Ribose	127:134	D-Ribose	127:134	D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability.
26759810	1	10	used	used	173:176	arg2	source					191:196	an energy source	181:196	an energy source	181:196	D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability.
26759810	0	11	theme	Bacillus	100:107	arg1	UJS0717					118:124	Bacillus subtilis UJS0717	100:124	Bacillus subtilis UJS0717	100:124	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
26759810	0	12	from	Hydrolysate	52:62	arg1	Improvement					0:10	Improvement	0:10	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain	0:98	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
26759810	0	12	from	Hydrolysate	52:62	arg1	Production					24:33	D-Ribose Production	15:33	D-Ribose Production from Corn Starch Hydrolysate	15:62	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
26759810	0	13	dep	UJS0717	118:124	arg1	Improvement					0:10	Improvement	0:10	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain	0:98	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
26759810	2	14	theme	production	291:300	arg1	performance					302:312	maximum D-ribose production performance	274:312	maximum D-ribose production performance from cheap raw material corn starch hydrolysate	274:360	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	2	15	theme	corn	338:341	arg1	hydrolysate					350:360	corn starch hydrolysate	338:360	cheap raw material corn starch hydrolysate	319:360	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	3	16	theme	D-ribose	760:767	arg1	production					769:778	the optimized D-ribose production	746:778	the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h	746:888	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	17	theme	1 g/L	1011:1015	arg1	extract					1023:1029	1 g/L yeast extract	1011:1029	1 g/L yeast extract	1011:1029	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	17	theme	1 g/L	1011:1015	arg1	compositions					924:935	the medium compositions	913:935	the medium compositions	913:935	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	2	18	theme	D-ribose	282:289	arg1	performance					302:312	maximum D-ribose production performance	274:312	maximum D-ribose production performance from cheap raw material corn starch hydrolysate	274:360	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	3	19	theme	157 g/L	949:955	arg1	glucose					957:963	157 g/L glucose	949:963	157 g/L glucose	949:963	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	19	theme	157 g/L	949:955	arg1	compositions					924:935	the medium compositions	913:935	the medium compositions	913:935	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	2	20	theme	three-level	424:434	arg1	design					458:463	a three-level Box-Behnken factorial design	422:463	a three-level Box-Behnken factorial design	422:463	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	3	21	theme	volumetric	852:861	arg1	productivity					863:874	volumetric productivity	852:874	volumetric productivity of 0.86 g/L·h	852:888	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	0	22	dep	Bacillus	100:107	arg1	subtilis					109:116	subtilis	109:116	subtilis	109:116	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
26759810	3	23	with	performance	780:790	arg1	concentration					801:813	the concentration	797:813	the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h	797:888	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	1	24	theme	energy	184:189	arg1	D-Ribose					127:134	D-Ribose	127:134	D-Ribose	127:134	D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability.
26759810	1	24	theme	energy	184:189	arg1	source					191:196	an energy source	181:196	an energy source	181:196	D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability.
26759810	3	25	dep	pH.	582:584	arg1	developed					618:626	developed	618:626	was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3	609:1067	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	2	26	theme	raw	325:327	arg1	material					329:336	cheap raw material	319:336	cheap raw material corn starch hydrolysate	319:360	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	3	27	theme	21 g/L	966:971	arg1	liquor					984:989	21 g/L corn steep liquor	966:989	21 g/L corn steep liquor	966:989	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	27	theme	21 g/L	966:971	arg1	compositions					924:935	the medium compositions	913:935	the medium compositions	913:935	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	28	theme	optimal	470:476	arg1	pH.					582:584	7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3	570:1067	7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3	570:1067	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	28	theme	optimal	470:476	arg1	parameters					491:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	28	theme	optimal	470:476	arg1	volume					558:563	10% inoculum volume	545:563	10% inoculum volume	545:563	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	28	theme	optimal	470:476	arg1	temperature					532:542	36°C culture temperature	519:542	36°C culture temperature	519:542	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	0	29	theme	Production	24:33	arg1	Improvement					0:10	Improvement	0:10	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain	0:98	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
26759810	3	30	theme	D-ribose	718:725	arg1	production					704:713	the production	700:713	the production of D-ribose	700:725	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	31	theme	0.05 g/L	1032:1039	arg1	MnSO4·H2O					1041:1049	0.05 g/L MnSO4·H2O	1032:1049	0.05 g/L MnSO4·H2O	1032:1049	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	31	theme	0.05 g/L	1032:1039	arg1	compositions					924:935	the medium compositions	913:935	the medium compositions	913:935	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	2	32	theme	cheap	319:323	arg1	material					329:336	cheap raw material	319:336	cheap raw material corn starch hydrolysate	319:360	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	3	33	theme	corn	973:976	arg1	liquor					984:989	21 g/L corn steep liquor	966:989	21 g/L corn steep liquor	966:989	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	33	theme	corn	973:976	arg1	compositions					924:935	the medium compositions	913:935	the medium compositions	913:935	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	0	34	theme	D-Ribose	15:22	arg1	Production					24:33	D-Ribose Production	15:33	D-Ribose Production from Corn Starch Hydrolysate	15:62	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
26759810	3	35	theme	production	769:778	arg1	performance					780:790	the optimized D-ribose production performance	746:790	the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h	746:888	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	36	theme	mathematical	590:601	arg1	model					603:607	The mathematical model	586:607	The mathematical model	586:607	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	37	theme	36°C	519:522	arg1	parameters					491:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	37	theme	36°C	519:522	arg1	temperature					532:542	36°C culture temperature	519:542	36°C culture temperature	519:542	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	0	38	theme	Corn	40:43	arg1	Hydrolysate					52:62	Corn Starch Hydrolysate	40:62	Corn Starch Hydrolysate	40:62	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
26759810	3	39	from	effect	640:645	arg1	production					704:713	the production	700:713	the production of D-ribose	700:725	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	2	40	theme	factorial	448:456	arg1	design					458:463	a three-level Box-Behnken factorial design	422:463	a three-level Box-Behnken factorial design	422:463	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	1	41	theme	athletic	209:216	arg1	performance					218:228	athletic performance	209:228	athletic performance	209:228	D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability.
26759810	3	42	theme	fermentation	478:489	arg1	pH.					582:584	7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3	570:1067	7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3	570:1067	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	42	theme	fermentation	478:489	arg1	parameters					491:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	42	theme	fermentation	478:489	arg1	volume					558:563	10% inoculum volume	545:563	10% inoculum volume	545:563	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	42	theme	fermentation	478:489	arg1	temperature					532:542	36°C culture temperature	519:542	36°C culture temperature	519:542	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	43	theme	62.13 g/L	818:826	arg1	concentration					801:813	the concentration	797:813	the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h	797:888	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	44	theme	medium	655:660	arg1	composition					662:672	each medium composition	650:672	each medium composition	650:672	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	2	45	theme	Box-Behnken	436:446	arg1	design					458:463	a three-level Box-Behnken factorial design	422:463	a three-level Box-Behnken factorial design	422:463	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	3	46	theme	composition	662:672	arg1	effect					640:645	the effect	636:645	the effect of each medium composition	636:672	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	46	theme	composition	662:672	arg1	interactions					684:695	their interactions	678:695	their interactions on the production of D-ribose	678:725	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	47	theme	3.2 g/L	992:998	arg1	NH4					1001:1003	3.2 g/L (NH4)2SO4	992:1008	3.2 g/L (NH4)2SO4	992:1008	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	47	theme	3.2 g/L	992:998	arg1	compositions					924:935	the medium compositions	913:935	the medium compositions	913:935	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	0	48	theme	Starch	45:50	arg1	Hydrolysate					52:62	Corn Starch Hydrolysate	40:62	Corn Starch Hydrolysate	40:62	Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient Strain Bacillus subtilis UJS0717.
26759810	3	49	theme	culture	524:530	arg1	parameters					491:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters	466:500	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	49	theme	culture	524:530	arg1	temperature					532:542	36°C culture temperature	519:542	36°C culture temperature	519:542	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	50	theme	steep	978:982	arg1	liquor					984:989	21 g/L corn steep liquor	966:989	21 g/L corn steep liquor	966:989	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	50	theme	steep	978:982	arg1	compositions					924:935	the medium compositions	913:935	the medium compositions	913:935	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	51	theme	10	545:546	arg1	%					547:547	%	547:547	%	547:547	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	2	52	theme	starch	343:348	arg1	hydrolysate					350:360	corn starch hydrolysate	338:360	cheap raw material corn starch hydrolysate	319:360	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	3	53	theme	0.40 g/g	838:845	arg1	productivity					863:874	volumetric productivity	852:874	volumetric productivity of 0.86 g/L·h	852:888	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	53	theme	0.40 g/g	838:845	arg1	yield					829:833	yield	829:833	yield of 0.40 g/g	829:845	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	53	theme	0.40 g/g	838:845	arg1	62.13 g/L					818:826	62.13 g/L	818:826	62.13 g/L	818:826	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	2	54	theme	maximum	274:280	arg1	performance					302:312	maximum D-ribose production performance	274:312	maximum D-ribose production performance from cheap raw material corn starch hydrolysate	274:360	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	3	55	theme	20 g/L	1056:1061	arg1	CaCO3					1063:1067	20 g/L CaCO3	1056:1067	20 g/L CaCO3	1056:1067	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	55	theme	20 g/L	1056:1061	arg1	compositions					924:935	the medium compositions	913:935	the medium compositions	913:935	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	1	56	theme	five-carbon	141:151	arg1	sugar					153:157	a five-carbon sugar	139:157	a five-carbon sugar	139:157	D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability.
26759810	3	57	theme	0.86 g/L·h	879:888	arg1	productivity					863:874	volumetric productivity	852:874	volumetric productivity of 0.86 g/L·h	852:888	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	57	theme	0.86 g/L·h	879:888	arg1	yield					829:833	yield	829:833	yield of 0.40 g/g	829:845	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	57	theme	0.86 g/L·h	879:888	arg1	62.13 g/L					818:826	62.13 g/L	818:826	62.13 g/L	818:826	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	2	58	dep	material	329:336	arg1	hydrolysate					350:360	corn starch hydrolysate	338:360	cheap raw material corn starch hydrolysate	319:360	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	3	59	from	interactions	684:695	arg1	production					704:713	the production	700:713	the production of D-ribose	700:725	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	2	60	theme	culture	251:257	arg1	conditions					259:268	The culture conditions	247:268	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate	247:360	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	2	61	theme	one-factor-at-a-time	385:404	arg1	experiments					406:416	one-factor-at-a-time experiments	385:416	one-factor-at-a-time experiments	385:416	The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design.
26759810	3	62	theme	yield	829:833	arg1	concentration					801:813	the concentration	797:813	the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h	797:888	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	4	63	theme	production	1108:1117	arg1	performance					1119:1129	the D-ribose production performance	1095:1129	the D-ribose production performance	1095:1129	These findings indicated the D-ribose production performance was significantly improved compared to that under original conditions.
26759810	3	64	theme	medium	917:922	arg1	extract					1023:1029	1 g/L yeast extract	1011:1029	1 g/L yeast extract	1011:1029	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	64	theme	medium	917:922	arg1	CaCO3					1063:1067	20 g/L CaCO3	1056:1067	20 g/L CaCO3	1056:1067	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	64	theme	medium	917:922	arg1	NH4					1001:1003	3.2 g/L (NH4)2SO4	992:1008	3.2 g/L (NH4)2SO4	992:1008	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	64	theme	medium	917:922	arg1	MnSO4·H2O					1041:1049	0.05 g/L MnSO4·H2O	1032:1049	0.05 g/L MnSO4·H2O	1032:1049	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	64	theme	medium	917:922	arg1	liquor					984:989	21 g/L corn steep liquor	966:989	21 g/L corn steep liquor	966:989	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	64	theme	medium	917:922	arg1	glucose					957:963	157 g/L glucose	949:963	157 g/L glucose	949:963	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26759810	3	64	theme	medium	917:922	arg1	compositions					924:935	the medium compositions	913:935	the medium compositions	913:935	The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3.
26064926	8	0	from	carmellose	1323:1332	arg1	form					1341:1344	the form	1337:1344	the form of nonwoven textile in its structure	1337:1381	Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure.
26064926	1	1	theme	oral	197:200	arg1	ailments					209:216	the most common oral cavity ailments	181:216	the most common oral cavity ailments	181:216	Infectious stomatitis represents the most common oral cavity ailments.
26064926	6	2	theme	medical	990:996	arg1	preparation					998:1008	the medical preparation	986:1008	the medical preparation	986:1008	The multivariate data analysis was employed to evaluate the influence of the formulation and process variables on the properties of the medical preparation.
26064926	9	3	theme	Candida	1530:1536	arg1	growth					1501:1506	the growth	1497:1506	the growth of Staphylococcus and Candida	1497:1536	In addition to its promising physicomechanical, chemical, and mucoadhesive properties, the formulation inhibited the growth of Staphylococcus and Candida; the effect was prolonged for tens of hours.
26064926	4	4	theme	nanocomposite	576:588	arg1	biomaterial					590:600	incorporated nanocomposite biomaterial	563:600	incorporated nanocomposite biomaterial that is able to release the drug directly at the target area	563:661	This formulation consists of a mucoadhesive oral film with incorporated nanocomposite biomaterial that is able to release the drug directly at the target area.
26064926	1	5	theme	cavity	202:207	arg1	ailments					209:216	the most common oral cavity ailments	181:216	the most common oral cavity ailments	181:216	Infectious stomatitis represents the most common oral cavity ailments.
26064926	0	6	theme	Oral	131:134	arg1	Infections					136:145	Oral Infections	131:145	Oral Infections	131:145	Carmellose Mucoadhesive Oral Films Containing Vermiculite/Chlorhexidine Nanocomposites as Innovative Biomaterials for Treatment of Oral Infections.
26064926	4	7	theme	incorporated	563:574	arg1	biomaterial					590:600	incorporated nanocomposite biomaterial	563:600	incorporated nanocomposite biomaterial that is able to release the drug directly at the target area	563:661	This formulation consists of a mucoadhesive oral film with incorporated nanocomposite biomaterial that is able to release the drug directly at the target area.
26064926	5	8	theme	mucoadhesive	718:729	arg1	films					736:740	mucoadhesive oral films	718:740	mucoadhesive oral films from carmellose	718:756	This study describes the unique approach of preparing mucoadhesive oral films from carmellose with incorporating a nanotechnologically modified clay mineral intercalated with chlorhexidine.
26064926	7	9	theme	antimycotic	1077:1087	arg1	activity					1089:1096	the antimicrobial and antimycotic activity	1055:1096	the antimicrobial and antimycotic activity of prepared films	1055:1114	This evaluation was complemented by testing the antimicrobial and antimycotic activity of prepared films with the aim of finding the most suitable composition for clinical application.
26064926	2	10	theme	formulations	338:349	arg1	time					294:297	the short residence time	274:297	the short residence time of topical liquid or semisolid medical formulations	274:349	Current therapy is insufficiently effective because of the short residence time of topical liquid or semisolid medical formulations.
26064926	4	11	theme	target	651:656	arg1	area					658:661	the target area	647:661	the target area	647:661	This formulation consists of a mucoadhesive oral film with incorporated nanocomposite biomaterial that is able to release the drug directly at the target area.
26064926	3	12	theme	residence	433:441	arg1	time					443:446	prolonged residence time	423:446	prolonged residence time on the oral mucosa	423:465	An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa may be a solution to this challenge.
26064926	3	13	from	time	443:446	arg1	mucosa					460:465	the oral mucosa	451:465	the oral mucosa	451:465	An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa may be a solution to this challenge.
26064926	5	14	theme	oral	731:734	arg1	films					736:740	mucoadhesive oral films	718:740	mucoadhesive oral films from carmellose	718:756	This study describes the unique approach of preparing mucoadhesive oral films from carmellose with incorporating a nanotechnologically modified clay mineral intercalated with chlorhexidine.
26064926	2	15	theme	medical	330:336	arg1	formulations					338:349	topical liquid or semisolid medical formulations	302:349	topical liquid or semisolid medical formulations	302:349	Current therapy is insufficiently effective because of the short residence time of topical liquid or semisolid medical formulations.
26064926	7	16	theme	antimicrobial	1059:1071	arg1	activity					1089:1096	the antimicrobial and antimycotic activity	1055:1096	the antimicrobial and antimycotic activity of prepared films	1055:1114	This evaluation was complemented by testing the antimicrobial and antimycotic activity of prepared films with the aim of finding the most suitable composition for clinical application.
26064926	2	17	theme	semisolid	320:328	arg1	formulations					338:349	topical liquid or semisolid medical formulations	302:349	topical liquid or semisolid medical formulations	302:349	Current therapy is insufficiently effective because of the short residence time of topical liquid or semisolid medical formulations.
26064926	8	18	theme	nonwoven	1349:1356	arg1	form					1341:1344	the form	1337:1344	the form of nonwoven textile in its structure	1337:1381	Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure.
26064926	0	19	theme	Infections	136:145	arg1	Treatment					118:126	Treatment	118:126	Treatment of Oral Infections	118:145	Carmellose Mucoadhesive Oral Films Containing Vermiculite/Chlorhexidine Nanocomposites as Innovative Biomaterials for Treatment of Oral Infections.
26064926	4	20	theme	oral	548:551	arg1	film					553:556	a mucoadhesive oral film	533:556	a mucoadhesive oral film with incorporated nanocomposite biomaterial that is able to release the drug directly at the target area	533:661	This formulation consists of a mucoadhesive oral film with incorporated nanocomposite biomaterial that is able to release the drug directly at the target area.
26064926	6	21	theme	process	947:953	arg1	variables					955:963	the formulation and process variables	927:963	the formulation and process variables	927:963	The multivariate data analysis was employed to evaluate the influence of the formulation and process variables on the properties of the medical preparation.
26064926	3	22	theme	oral	455:458	arg1	mucosa					460:465	the oral mucosa	451:465	the oral mucosa	451:465	An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa may be a solution to this challenge.
26064926	0	23	theme	Mucoadhesive	11:22	arg1	Films					29:33	Carmellose Mucoadhesive Oral Films	0:33	Carmellose Mucoadhesive Oral Films	0:33	Carmellose Mucoadhesive Oral Films Containing Vermiculite/Chlorhexidine Nanocomposites as Innovative Biomaterials for Treatment of Oral Infections.
26064926	4	24	theme	mucoadhesive	535:546	arg1	film					553:556	a mucoadhesive oral film	533:556	a mucoadhesive oral film with incorporated nanocomposite biomaterial that is able to release the drug directly at the target area	533:661	This formulation consists of a mucoadhesive oral film with incorporated nanocomposite biomaterial that is able to release the drug directly at the target area.
26064926	5	25	theme	unique	689:694	arg1	approach					696:703	the unique approach	685:703	the unique approach of preparing mucoadhesive oral films from carmellose with incorporating a nanotechnologically modified clay mineral intercalated with chlorhexidine	685:851	This study describes the unique approach of preparing mucoadhesive oral films from carmellose with incorporating a nanotechnologically modified clay mineral intercalated with chlorhexidine.
26064926	8	26	contain	containing	1250:1259	arg2	20 mg					1261:1265	20 mg	1261:1265	20 mg of chlorhexidine diacetate carried by vermiculite	1261:1315	Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure.
26064926	8	26	contain	containing	1250:1259	arg1	sample					1243:1248	sample	1243:1248	sample containing 20 mg of chlorhexidine diacetate carried by vermiculite	1243:1315	Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure.
26064926	7	27	theme	suitable	1149:1156	arg1	composition					1158:1168	the most suitable composition	1140:1168	the most suitable composition	1140:1168	This evaluation was complemented by testing the antimicrobial and antimycotic activity of prepared films with the aim of finding the most suitable composition for clinical application.
26064926	6	28	from	influence	914:922	arg1	properties					972:981	the properties	968:981	the properties of the medical preparation	968:1008	The multivariate data analysis was employed to evaluate the influence of the formulation and process variables on the properties of the medical preparation.
26064926	0	29	theme	Carmellose	0:9	arg1	Films					29:33	Carmellose Mucoadhesive Oral Films	0:33	Carmellose Mucoadhesive Oral Films	0:33	Carmellose Mucoadhesive Oral Films Containing Vermiculite/Chlorhexidine Nanocomposites as Innovative Biomaterials for Treatment of Oral Infections.
26064926	8	30	theme	best	1211:1214	arg1	results					1216:1222	the best results	1207:1222	the best results	1207:1222	Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure.
26064926	8	31	theme	textile	1358:1364	arg1	nonwoven					1349:1356	nonwoven textile	1349:1364	nonwoven textile in its structure	1349:1381	Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure.
26064926	9	32	theme	mucoadhesive	1446:1457	arg1	properties					1459:1468	its promising physicomechanical, chemical, and mucoadhesive properties	1399:1468	its promising physicomechanical, chemical, and mucoadhesive properties	1399:1468	In addition to its promising physicomechanical, chemical, and mucoadhesive properties, the formulation inhibited the growth of Staphylococcus and Candida; the effect was prolonged for tens of hours.
26064926	7	33	theme	clinical	1174:1181	arg1	application					1183:1193	clinical application	1174:1193	clinical application	1174:1193	This evaluation was complemented by testing the antimicrobial and antimycotic activity of prepared films with the aim of finding the most suitable composition for clinical application.
26064926	6	34	theme	data	871:874	arg1	analysis					876:883	The multivariate data analysis	854:883	The multivariate data analysis	854:883	The multivariate data analysis was employed to evaluate the influence of the formulation and process variables on the properties of the medical preparation.
26064926	0	35	theme	Oral	24:27	arg1	Films					29:33	Carmellose Mucoadhesive Oral Films	0:33	Carmellose Mucoadhesive Oral Films	0:33	Carmellose Mucoadhesive Oral Films Containing Vermiculite/Chlorhexidine Nanocomposites as Innovative Biomaterials for Treatment of Oral Infections.
26064926	8	36	theme	chlorhexidine	1270:1282	arg1	diacetate					1284:1292	chlorhexidine diacetate	1270:1292	chlorhexidine diacetate carried by vermiculite	1270:1315	Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure.
26064926	9	37	theme	hours	1576:1580	arg1	tens					1568:1571	tens	1568:1571	tens of hours	1568:1580	In addition to its promising physicomechanical, chemical, and mucoadhesive properties, the formulation inhibited the growth of Staphylococcus and Candida; the effect was prolonged for tens of hours.
26064926	0	38	theme	Vermiculite/Chlorhexidine	46:70	arg1	Nanocomposites					72:85	Vermiculite/Chlorhexidine Nanocomposites	46:85	Vermiculite/Chlorhexidine Nanocomposites	46:85	Carmellose Mucoadhesive Oral Films Containing Vermiculite/Chlorhexidine Nanocomposites as Innovative Biomaterials for Treatment of Oral Infections.
26064926	0	38	theme	Vermiculite/Chlorhexidine	46:70	arg1	Biomaterials					101:112	Innovative Biomaterials	90:112	Innovative Biomaterials for Treatment of Oral Infections	90:145	Carmellose Mucoadhesive Oral Films Containing Vermiculite/Chlorhexidine Nanocomposites as Innovative Biomaterials for Treatment of Oral Infections.
26064926	2	39	theme	Current	219:225	arg1	therapy					227:233	Current therapy	219:233	Current therapy	219:233	Current therapy is insufficiently effective because of the short residence time of topical liquid or semisolid medical formulations.
26064926	2	40	theme	residence	284:292	arg1	time					294:297	the short residence time	274:297	the short residence time of topical liquid or semisolid medical formulations	274:349	Current therapy is insufficiently effective because of the short residence time of topical liquid or semisolid medical formulations.
26064926	5	41	theme	modified	799:806	arg1	clay					808:811	a nanotechnologically modified clay mineral	777:819	a nanotechnologically modified clay mineral intercalated with chlorhexidine	777:851	This study describes the unique approach of preparing mucoadhesive oral films from carmellose with incorporating a nanotechnologically modified clay mineral intercalated with chlorhexidine.
26064926	6	42	theme	variables	955:963	arg1	influence					914:922	the influence	910:922	the influence of the formulation and process variables on the properties of the medical preparation	910:1008	The multivariate data analysis was employed to evaluate the influence of the formulation and process variables on the properties of the medical preparation.
26064926	2	43	theme	short	278:282	arg1	time					294:297	the short residence time	274:297	the short residence time of topical liquid or semisolid medical formulations	274:349	Current therapy is insufficiently effective because of the short residence time of topical liquid or semisolid medical formulations.
26064926	6	44	theme	multivariate	858:869	arg1	analysis					876:883	The multivariate data analysis	854:883	The multivariate data analysis	854:883	The multivariate data analysis was employed to evaluate the influence of the formulation and process variables on the properties of the medical preparation.
26064926	8	45	theme	diacetate	1284:1292	arg1	20 mg					1261:1265	20 mg	1261:1265	20 mg of chlorhexidine diacetate carried by vermiculite	1261:1315	Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure.
26064926	3	46	theme	prolonged	423:431	arg1	time					443:446	prolonged residence time	423:446	prolonged residence time on the oral mucosa	423:465	An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa may be a solution to this challenge.
26064926	3	47	theme	innovative	355:364	arg1	form					378:381	An innovative application form	352:381	An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa	352:465	An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa may be a solution to this challenge.
26064926	3	47	theme	innovative	355:364	arg1	solution					476:483	a solution	474:483	a solution to this challenge	474:501	An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa may be a solution to this challenge.
26064926	3	48	theme	bioadhesive	392:402	arg1	polymers					404:411	bioadhesive polymers	392:411	bioadhesive polymers featuring prolonged residence time on the oral mucosa	392:465	An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa may be a solution to this challenge.
26064926	9	49	theme	chemical	1432:1439	arg1	properties					1459:1468	its promising physicomechanical, chemical, and mucoadhesive properties	1399:1468	its promising physicomechanical, chemical, and mucoadhesive properties	1399:1468	In addition to its promising physicomechanical, chemical, and mucoadhesive properties, the formulation inhibited the growth of Staphylococcus and Candida; the effect was prolonged for tens of hours.
26064926	1	50	theme	Infectious	148:157	arg1	stomatitis					159:168	Infectious stomatitis	148:168	Infectious stomatitis	148:168	Infectious stomatitis represents the most common oral cavity ailments.
26064926	6	51	theme	preparation	998:1008	arg1	properties					972:981	the properties	968:981	the properties of the medical preparation	968:1008	The multivariate data analysis was employed to evaluate the influence of the formulation and process variables on the properties of the medical preparation.
26064926	8	52	from	form	1341:1344	arg1	structure					1373:1381	its structure	1369:1381	its structure	1369:1381	Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure.
26064926	3	53	theme	application	366:376	arg1	form					378:381	An innovative application form	352:381	An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa	352:465	An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa may be a solution to this challenge.
26064926	3	53	theme	application	366:376	arg1	solution					476:483	a solution	474:483	a solution to this challenge	474:501	An innovative application form based on bioadhesive polymers featuring prolonged residence time on the oral mucosa may be a solution to this challenge.
26064926	8	54	from	nonwoven	1349:1356	arg1	structure					1373:1381	its structure	1369:1381	its structure	1369:1381	Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure.
26064926	6	55	theme	formulation	931:941	arg1	variables					955:963	the formulation and process variables	927:963	the formulation and process variables	927:963	The multivariate data analysis was employed to evaluate the influence of the formulation and process variables on the properties of the medical preparation.
26064926	2	56	theme	liquid	310:315	arg1	formulations					338:349	topical liquid or semisolid medical formulations	302:349	topical liquid or semisolid medical formulations	302:349	Current therapy is insufficiently effective because of the short residence time of topical liquid or semisolid medical formulations.
26064926	5	57	from	carmellose	747:756	arg1	films					736:740	mucoadhesive oral films	718:740	mucoadhesive oral films from carmellose	718:756	This study describes the unique approach of preparing mucoadhesive oral films from carmellose with incorporating a nanotechnologically modified clay mineral intercalated with chlorhexidine.
26064926	9	58	theme	promising	1403:1411	arg1	properties					1459:1468	its promising physicomechanical, chemical, and mucoadhesive properties	1399:1468	its promising physicomechanical, chemical, and mucoadhesive properties	1399:1468	In addition to its promising physicomechanical, chemical, and mucoadhesive properties, the formulation inhibited the growth of Staphylococcus and Candida; the effect was prolonged for tens of hours.
26064926	2	59	theme	topical	302:308	arg1	formulations					338:349	topical liquid or semisolid medical formulations	302:349	topical liquid or semisolid medical formulations	302:349	Current therapy is insufficiently effective because of the short residence time of topical liquid or semisolid medical formulations.
26064926	7	60	theme	prepared	1101:1108	arg1	films					1110:1114	prepared films	1101:1114	prepared films	1101:1114	This evaluation was complemented by testing the antimicrobial and antimycotic activity of prepared films with the aim of finding the most suitable composition for clinical application.
26064926	4	61	with	film	553:556	arg1	biomaterial					590:600	incorporated nanocomposite biomaterial	563:600	incorporated nanocomposite biomaterial that is able to release the drug directly at the target area	563:661	This formulation consists of a mucoadhesive oral film with incorporated nanocomposite biomaterial that is able to release the drug directly at the target area.
26064926	7	62	theme	films	1110:1114	arg1	activity					1089:1096	the antimicrobial and antimycotic activity	1055:1096	the antimicrobial and antimycotic activity of prepared films	1055:1114	This evaluation was complemented by testing the antimicrobial and antimycotic activity of prepared films with the aim of finding the most suitable composition for clinical application.
26064926	9	63	theme	Staphylococcus	1511:1524	arg1	growth					1501:1506	the growth	1497:1506	the growth of Staphylococcus and Candida	1497:1536	In addition to its promising physicomechanical, chemical, and mucoadhesive properties, the formulation inhibited the growth of Staphylococcus and Candida; the effect was prolonged for tens of hours.
26064926	0	64	theme	Innovative	90:99	arg1	Nanocomposites					72:85	Vermiculite/Chlorhexidine Nanocomposites	46:85	Vermiculite/Chlorhexidine Nanocomposites	46:85	Carmellose Mucoadhesive Oral Films Containing Vermiculite/Chlorhexidine Nanocomposites as Innovative Biomaterials for Treatment of Oral Infections.
26064926	0	64	theme	Innovative	90:99	arg1	Biomaterials					101:112	Innovative Biomaterials	90:112	Innovative Biomaterials for Treatment of Oral Infections	90:145	Carmellose Mucoadhesive Oral Films Containing Vermiculite/Chlorhexidine Nanocomposites as Innovative Biomaterials for Treatment of Oral Infections.
26064926	9	65	theme	physicomechanical	1413:1429	arg1	properties					1459:1468	its promising physicomechanical, chemical, and mucoadhesive properties	1399:1468	its promising physicomechanical, chemical, and mucoadhesive properties	1399:1468	In addition to its promising physicomechanical, chemical, and mucoadhesive properties, the formulation inhibited the growth of Staphylococcus and Candida; the effect was prolonged for tens of hours.
26064926	8	66	from	structure	1373:1381	arg1	form					1341:1344	the form	1337:1344	the form of nonwoven textile in its structure	1337:1381	Generally, the best results were obtained with sample containing 20 mg of chlorhexidine diacetate carried by vermiculite, with carmellose in the form of nonwoven textile in its structure.
26064926	1	67	theme	common	190:195	arg1	ailments					209:216	the most common oral cavity ailments	181:216	the most common oral cavity ailments	181:216	Infectious stomatitis represents the most common oral cavity ailments.
26064926	5	68	theme	mineral	813:819	arg1	clay					808:811	a nanotechnologically modified clay mineral	777:819	a nanotechnologically modified clay mineral intercalated with chlorhexidine	777:851	This study describes the unique approach of preparing mucoadhesive oral films from carmellose with incorporating a nanotechnologically modified clay mineral intercalated with chlorhexidine.
26385331	4	0	theme	exoskeleton	505:515	arg1	parts					483:487	other parts	477:487	other parts of the crayfish exoskeleton, which is periodically shed and regenerated during the molt cycle	477:581	Unlike other parts of the crayfish exoskeleton, which is periodically shed and regenerated during the molt cycle, molar mineral deposition takes place during the pre-molt stage.
26385331	5	1	theme	molar	652:656	arg1	composition					666:676	The molar mineral composition	648:676	The molar mineral composition	648:676	The molar mineral composition transforms continuously from fluorapatite through amorphous calcium phosphate to amorphous calcium carbonate and is mounted on chitin.
26385331	0	2	with	protein	23:29	arg1	properties					86:95	putative mineralized exoskeletal chitinous matrix properties	36:95	putative mineralized exoskeletal chitinous matrix properties	36:95	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.
26385331	4	3	theme	molar	584:588	arg1	deposition					598:607	molar mineral deposition	584:607	molar mineral deposition	584:607	Unlike other parts of the crayfish exoskeleton, which is periodically shed and regenerated during the molt cycle, molar mineral deposition takes place during the pre-molt stage.
26385331	0	4	theme	matrix	79:84	arg1	properties					86:95	putative mineralized exoskeletal chitinous matrix properties	36:95	putative mineralized exoskeletal chitinous matrix properties	36:95	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.
26385331	6	5	theme	weight	957:962	arg1	molecules					964:972	low-molecular weight molecules	943:972	low-molecular weight molecules	943:972	The process of crayfish molar formation is entirely extracellular and presumably controlled by proteins, lipids, polysaccharides, low-molecular weight molecules and calcium salts.
26385331	5	6	theme	mineral	658:664	arg1	composition					666:676	The molar mineral composition	648:676	The molar mineral composition	648:676	The molar mineral composition transforms continuously from fluorapatite through amorphous calcium phosphate to amorphous calcium carbonate and is mounted on chitin.
26385331	11	7	theme	arthropod	1427:1435	arg1	proteins					1447:1454	other arthropod cuticular proteins	1421:1454	other arthropod cuticular proteins	1421:1454	Like other arthropod cuticular proteins, Cq-M15 possesses a chitin-binding Rebers-Riddiford domain, with a recombinant version of the protein found to bind chitin.
26385331	9	8	theme	mineral	1303:1309	arg1	arrangement					1311:1321	mineral arrangement	1303:1321	mineral arrangement	1303:1321	The predicted acidic pI of Cq-M15 suggests its possible involvement in mineral arrangement.
26385331	3	9	theme	vertebrate	365:374	arg1	enamel					376:381	vertebrate enamel	365:381	vertebrate enamel	365:381	As this layer resembles vertebrate enamel in composition and function, it offers an interesting example of convergent evolution.
26385331	6	10	theme	low-molecular	943:955	arg1	molecules					964:972	low-molecular weight molecules	943:972	low-molecular weight molecules	943:972	The process of crayfish molar formation is entirely extracellular and presumably controlled by proteins, lipids, polysaccharides, low-molecular weight molecules and calcium salts.
26385331	7	11	theme	novel	1014:1018	arg1	protein					1026:1032	a novel molar protein	1012:1032	a novel molar protein termed Cq-M15 from C. quadricarinatus	1012:1070	We have identified a novel molar protein termed Cq-M15 from C. quadricarinatus and cloned its transcript from the molar-forming epithelium.
26385331	6	12	theme	formation	843:851	arg1	process					817:823	The process	813:823	The process of crayfish molar formation	813:851	The process of crayfish molar formation is entirely extracellular and presumably controlled by proteins, lipids, polysaccharides, low-molecular weight molecules and calcium salts.
26385331	9	13	theme	acidic	1246:1251	arg1	pI					1253:1254	The predicted acidic pI	1232:1254	The predicted acidic pI of Cq-M15	1232:1264	The predicted acidic pI of Cq-M15 suggests its possible involvement in mineral arrangement.
26385331	7	14	from	epithelium	1121:1130	arg1	transcript					1087:1096	its transcript	1083:1096	its transcript from the molar-forming epithelium	1083:1130	We have identified a novel molar protein termed Cq-M15 from C. quadricarinatus and cloned its transcript from the molar-forming epithelium.
26385331	5	15	theme	calcium	769:775	arg1	carbonate					777:785	amorphous calcium carbonate	759:785	amorphous calcium carbonate	759:785	The molar mineral composition transforms continuously from fluorapatite through amorphous calcium phosphate to amorphous calcium carbonate and is mounted on chitin.
26385331	13	16	theme	latter	1674:1679	arg1	property					1681:1688	This latter property	1669:1688	This latter property	1669:1688	This latter property might make Cq-M15 useful for bone and dental regenerative efforts.
26385331	6	17	theme	molar	837:841	arg1	formation					843:851	crayfish molar formation	828:851	crayfish molar formation	828:851	The process of crayfish molar formation is entirely extracellular and presumably controlled by proteins, lipids, polysaccharides, low-molecular weight molecules and calcium salts.
26385331	4	18	theme	mineral	590:596	arg1	deposition					598:607	molar mineral deposition	584:607	molar mineral deposition	584:607	Unlike other parts of the crayfish exoskeleton, which is periodically shed and regenerated during the molt cycle, molar mineral deposition takes place during the pre-molt stage.
26385331	11	19	theme	Rebers-Riddiford	1491:1506	arg1	domain					1508:1513	a chitin-binding Rebers-Riddiford domain	1474:1513	a chitin-binding Rebers-Riddiford domain	1474:1513	Like other arthropod cuticular proteins, Cq-M15 possesses a chitin-binding Rebers-Riddiford domain, with a recombinant version of the protein found to bind chitin.
26385331	6	20	theme	crayfish	828:835	arg1	formation					843:851	crayfish molar formation	828:851	crayfish molar formation	828:851	The process of crayfish molar formation is entirely extracellular and presumably controlled by proteins, lipids, polysaccharides, low-molecular weight molecules and calcium salts.
26385331	14	21	dep	phosphate	1836:1844	arg1	precipitation					1863:1875	precipitation	1863:1875	precipitation	1863:1875	We suggest that, in the molar tooth, this protein might be involved in calcium phosphate and/or carbonate precipitation.
26385331	11	22	theme	cuticular	1437:1445	arg1	proteins					1447:1454	other arthropod cuticular proteins	1421:1454	other arthropod cuticular proteins	1421:1454	Like other arthropod cuticular proteins, Cq-M15 possesses a chitin-binding Rebers-Riddiford domain, with a recombinant version of the protein found to bind chitin.
26385331	4	23	theme	crayfish	496:503	arg1	exoskeleton					505:515	the crayfish exoskeleton	492:515	the crayfish exoskeleton	492:515	Unlike other parts of the crayfish exoskeleton, which is periodically shed and regenerated during the molt cycle, molar mineral deposition takes place during the pre-molt stage.
26385331	9	24	theme	possible	1279:1286	arg1	involvement					1288:1298	its possible involvement	1275:1298	its possible involvement in mineral arrangement	1275:1321	The predicted acidic pI of Cq-M15 suggests its possible involvement in mineral arrangement.
26385331	13	25	theme	regenerative	1735:1746	arg1	efforts					1748:1754	dental regenerative efforts	1728:1754	dental regenerative efforts	1728:1754	This latter property might make Cq-M15 useful for bone and dental regenerative efforts.
26385331	2	26	theme	exoskeleton	269:279	arg1	tooth					230:234	the molar tooth	220:234	the molar tooth	220:234	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	2	26	theme	exoskeleton	269:279	arg1	part					246:249	part	246:249	part of the mandibular exoskeleton	246:279	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	3	27	theme	convergent	448:457	arg1	evolution					459:467	convergent evolution	448:467	convergent evolution	448:467	As this layer resembles vertebrate enamel in composition and function, it offers an interesting example of convergent evolution.
26385331	0	28	theme	crayfish	2:9	arg1	protein					23:29	A crayfish molar tooth protein	0:29	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.	0:96	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.
26385331	8	29	theme	sequencing	1212:1221	arg1	library					1223:1229	a next-generation sequencing library	1194:1229	a next-generation sequencing library	1194:1229	Its transcript and differential expression were confirmed by a next-generation sequencing library.
26385331	8	30	theme	next-generation	1196:1210	arg1	library					1223:1229	a next-generation sequencing library	1194:1229	a next-generation sequencing library	1194:1229	Its transcript and differential expression were confirmed by a next-generation sequencing library.
26385331	2	31	theme	apatite	326:332	arg1	layer					334:338	an unusual crystalline enamel-like apatite layer	291:338	an unusual crystalline enamel-like apatite layer	291:338	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	10	32	theme	exoskeletal	1355:1365	arg1	tissues					1367:1373	several exoskeletal tissues	1347:1373	several exoskeletal tissues	1347:1373	Cq-M15 is expressed in several exoskeletal tissues at pre-molt and its silencing is lethal.
26385331	3	33	theme	interesting	425:435	arg1	example					437:443	an interesting example	422:443	an interesting example of convergent evolution	422:467	As this layer resembles vertebrate enamel in composition and function, it offers an interesting example of convergent evolution.
26385331	11	34	theme	other	1421:1425	arg1	proteins					1447:1454	other arthropod cuticular proteins	1421:1454	other arthropod cuticular proteins	1421:1454	Like other arthropod cuticular proteins, Cq-M15 possesses a chitin-binding Rebers-Riddiford domain, with a recombinant version of the protein found to bind chitin.
26385331	2	35	theme	enamel-like	314:324	arg1	layer					334:338	an unusual crystalline enamel-like apatite layer	291:338	an unusual crystalline enamel-like apatite layer	291:338	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	0	36	theme	tooth	17:21	arg1	protein					23:29	A crayfish molar tooth protein	0:29	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.	0:96	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.
26385331	5	37	theme	amorphous	728:736	arg1	phosphate					746:754	amorphous calcium phosphate	728:754	amorphous calcium phosphate to amorphous calcium carbonate	728:785	The molar mineral composition transforms continuously from fluorapatite through amorphous calcium phosphate to amorphous calcium carbonate and is mounted on chitin.
26385331	2	38	theme	crystalline	302:312	arg1	layer					334:338	an unusual crystalline enamel-like apatite layer	291:338	an unusual crystalline enamel-like apatite layer	291:338	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	0	39	theme	molar	11:15	arg1	protein					23:29	A crayfish molar tooth protein	0:29	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.	0:96	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.
26385331	2	40	contain	contains	282:289	arg1	tooth					230:234	the molar tooth	220:234	the molar tooth	220:234	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	2	40	contain	contains	282:289	arg2	part					246:249	part	246:249	part of the mandibular exoskeleton	246:279	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	2	40	contain	contains	282:289	arg2	layer					334:338	an unusual crystalline enamel-like apatite layer	291:338	an unusual crystalline enamel-like apatite layer	291:338	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	2	40	contain	contains	282:289	arg1	quadricarinatus					203:217	the crayfish Cherax quadricarinatus	183:217	the crayfish Cherax quadricarinatus	183:217	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	2	40	contain	contains	282:289	arg1	part					246:249	part	246:249	part of the mandibular exoskeleton	246:279	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	2	40	contain	contains	282:289	arg2	tooth					230:234	the molar tooth	220:234	the molar tooth	220:234	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	5	41	theme	calcium	738:744	arg1	phosphate					746:754	amorphous calcium phosphate	728:754	amorphous calcium phosphate to amorphous calcium carbonate	728:785	The molar mineral composition transforms continuously from fluorapatite through amorphous calcium phosphate to amorphous calcium carbonate and is mounted on chitin.
26385331	2	42	theme	Cherax	196:201	arg1	quadricarinatus					203:217	the crayfish Cherax quadricarinatus	183:217	the crayfish Cherax quadricarinatus	183:217	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	2	43	theme	unusual	294:300	arg1	layer					334:338	an unusual crystalline enamel-like apatite layer	291:338	an unusual crystalline enamel-like apatite layer	291:338	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	4	44	theme	pre-molt	632:639	arg1	stage					641:645	the pre-molt stage	628:645	the pre-molt stage	628:645	Unlike other parts of the crayfish exoskeleton, which is periodically shed and regenerated during the molt cycle, molar mineral deposition takes place during the pre-molt stage.
26385331	4	45	theme	molt	572:575	arg1	cycle					577:581	the molt cycle	568:581	the molt cycle	568:581	Unlike other parts of the crayfish exoskeleton, which is periodically shed and regenerated during the molt cycle, molar mineral deposition takes place during the pre-molt stage.
26385331	8	46	theme	differential	1152:1163	arg1	expression					1165:1174	differential expression	1152:1174	differential expression	1152:1174	Its transcript and differential expression were confirmed by a next-generation sequencing library.
26385331	2	47	theme	crayfish	187:194	arg1	quadricarinatus					203:217	the crayfish Cherax quadricarinatus	183:217	the crayfish Cherax quadricarinatus	183:217	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	11	48	theme	protein	1550:1556	arg1	version					1535:1541	a recombinant version	1521:1541	a recombinant version of the protein found to bind chitin	1521:1577	Like other arthropod cuticular proteins, Cq-M15 possesses a chitin-binding Rebers-Riddiford domain, with a recombinant version of the protein found to bind chitin.
26385331	3	49	theme	evolution	459:467	arg1	example					437:443	an interesting example	422:443	an interesting example of convergent evolution	422:467	As this layer resembles vertebrate enamel in composition and function, it offers an interesting example of convergent evolution.
26385331	1	50	contain	possess	115:121	arg2	exoskeletons					123:134	exoskeletons	123:134	exoskeletons that are reinforced with calcium carbonate	123:177	Some crustaceans possess exoskeletons that are reinforced with calcium carbonate.
26385331	1	50	contain	possess	115:121	arg1	crustaceans					103:113	Some crustaceans	98:113	Some crustaceans	98:113	Some crustaceans possess exoskeletons that are reinforced with calcium carbonate.
26385331	7	51	theme	molar-forming	1107:1119	arg1	epithelium					1121:1130	the molar-forming epithelium	1103:1130	the molar-forming epithelium	1103:1130	We have identified a novel molar protein termed Cq-M15 from C. quadricarinatus and cloned its transcript from the molar-forming epithelium.
26385331	4	52	theme	other	477:481	arg1	parts					483:487	other parts	477:487	other parts of the crayfish exoskeleton, which is periodically shed and regenerated during the molt cycle	477:581	Unlike other parts of the crayfish exoskeleton, which is periodically shed and regenerated during the molt cycle, molar mineral deposition takes place during the pre-molt stage.
26385331	0	53	theme	mineralized	45:55	arg1	properties					86:95	putative mineralized exoskeletal chitinous matrix properties	36:95	putative mineralized exoskeletal chitinous matrix properties	36:95	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.
26385331	5	54	theme	amorphous	759:767	arg1	carbonate					777:785	amorphous calcium carbonate	759:785	amorphous calcium carbonate	759:785	The molar mineral composition transforms continuously from fluorapatite through amorphous calcium phosphate to amorphous calcium carbonate and is mounted on chitin.
26385331	9	55	theme	Cq-M15	1259:1264	arg1	pI					1253:1254	The predicted acidic pI	1232:1254	The predicted acidic pI of Cq-M15	1232:1264	The predicted acidic pI of Cq-M15 suggests its possible involvement in mineral arrangement.
26385331	11	56	theme	chitin-binding	1476:1489	arg1	domain					1508:1513	a chitin-binding Rebers-Riddiford domain	1474:1513	a chitin-binding Rebers-Riddiford domain	1474:1513	Like other arthropod cuticular proteins, Cq-M15 possesses a chitin-binding Rebers-Riddiford domain, with a recombinant version of the protein found to bind chitin.
26385331	9	57	from	involvement	1288:1298	arg1	arrangement					1311:1321	mineral arrangement	1303:1321	mineral arrangement	1303:1321	The predicted acidic pI of Cq-M15 suggests its possible involvement in mineral arrangement.
26385331	0	58	theme	putative	36:43	arg1	properties					86:95	putative mineralized exoskeletal chitinous matrix properties	36:95	putative mineralized exoskeletal chitinous matrix properties	36:95	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.
26385331	11	59	theme	recombinant	1523:1533	arg1	version					1535:1541	a recombinant version	1521:1541	a recombinant version of the protein found to bind chitin	1521:1577	Like other arthropod cuticular proteins, Cq-M15 possesses a chitin-binding Rebers-Riddiford domain, with a recombinant version of the protein found to bind chitin.
26385331	2	60	theme	molar	224:228	arg1	tooth					230:234	the molar tooth	220:234	the molar tooth	220:234	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	2	60	theme	molar	224:228	arg1	part					246:249	part	246:249	part of the mandibular exoskeleton	246:279	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	11	61	contain	possesses	1464:1472	arg1	Cq-M15					1457:1462	Cq-M15	1457:1462	Cq-M15	1457:1462	Like other arthropod cuticular proteins, Cq-M15 possesses a chitin-binding Rebers-Riddiford domain, with a recombinant version of the protein found to bind chitin.
26385331	11	61	contain	possesses	1464:1472	arg2	domain					1508:1513	a chitin-binding Rebers-Riddiford domain	1474:1513	a chitin-binding Rebers-Riddiford domain	1474:1513	Like other arthropod cuticular proteins, Cq-M15 possesses a chitin-binding Rebers-Riddiford domain, with a recombinant version of the protein found to bind chitin.
26385331	14	62	theme	molar	1781:1785	arg1	tooth					1787:1791	the molar tooth	1777:1791	the molar tooth	1777:1791	We suggest that, in the molar tooth, this protein might be involved in calcium phosphate and/or carbonate precipitation.
26385331	6	63	theme	calcium	978:984	arg1	salts					986:990	calcium salts	978:990	calcium salts	978:990	The process of crayfish molar formation is entirely extracellular and presumably controlled by proteins, lipids, polysaccharides, low-molecular weight molecules and calcium salts.
26385331	2	64	theme	mandibular	258:267	arg1	exoskeleton					269:279	the mandibular exoskeleton	254:279	the mandibular exoskeleton	254:279	In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer.
26385331	7	65	theme	C.	1053:1054	arg1	quadricarinatus					1056:1070	C. quadricarinatus	1053:1070	C. quadricarinatus	1053:1070	We have identified a novel molar protein termed Cq-M15 from C. quadricarinatus and cloned its transcript from the molar-forming epithelium.
26385331	9	66	theme	predicted	1236:1244	arg1	pI					1253:1254	The predicted acidic pI	1232:1254	The predicted acidic pI of Cq-M15	1232:1264	The predicted acidic pI of Cq-M15 suggests its possible involvement in mineral arrangement.
26385331	13	67	theme	dental	1728:1733	arg1	efforts					1748:1754	dental regenerative efforts	1728:1754	dental regenerative efforts	1728:1754	This latter property might make Cq-M15 useful for bone and dental regenerative efforts.
26385331	14	68	theme	calcium	1828:1834	arg1	phosphate					1836:1844	calcium phosphate	1828:1844	calcium phosphate	1828:1844	We suggest that, in the molar tooth, this protein might be involved in calcium phosphate and/or carbonate precipitation.
26385331	0	69	theme	chitinous	69:77	arg1	properties					86:95	putative mineralized exoskeletal chitinous matrix properties	36:95	putative mineralized exoskeletal chitinous matrix properties	36:95	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.
26385331	12	70	theme	calcium	1619:1625	arg1	ions					1627:1630	calcium ions	1619:1630	calcium ions	1619:1630	Cq-M15 was also found to interact with calcium ions in a concentration-dependent manner.
26385331	0	71	theme	exoskeletal	57:67	arg1	properties					86:95	putative mineralized exoskeletal chitinous matrix properties	36:95	putative mineralized exoskeletal chitinous matrix properties	36:95	A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix properties.
26385331	7	72	theme	molar	1020:1024	arg1	protein					1026:1032	a novel molar protein	1012:1032	a novel molar protein termed Cq-M15 from C. quadricarinatus	1012:1070	We have identified a novel molar protein termed Cq-M15 from C. quadricarinatus and cloned its transcript from the molar-forming epithelium.
26385331	1	73	theme	calcium	161:167	arg1	carbonate					169:177	calcium carbonate	161:177	calcium carbonate	161:177	Some crustaceans possess exoskeletons that are reinforced with calcium carbonate.
26385331	10	74	theme	several	1347:1353	arg1	tissues					1367:1373	several exoskeletal tissues	1347:1373	several exoskeletal tissues	1347:1373	Cq-M15 is expressed in several exoskeletal tissues at pre-molt and its silencing is lethal.
26385331	12	75	theme	concentration-dependent	1637:1659	arg1	manner					1661:1666	a concentration-dependent manner	1635:1666	a concentration-dependent manner	1635:1666	Cq-M15 was also found to interact with calcium ions in a concentration-dependent manner.
27415597	0	0	theme	graphene	123:130	arg1	composite					132:140	coupled cMWCNT/cyclodextrin edge-functionalized graphene composite	75:140	coupled cMWCNT/cyclodextrin edge-functionalized graphene composite	75:140	Highly-sensitive electrocatalytic determination for toxic phenols based on coupled cMWCNT/cyclodextrin edge-functionalized graphene composite.
27415597	4	1	theme	architecture	1003:1014	arg1	performance					931:941	The electrocatalytic detection performance	900:941	The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs	900:1055	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	8	2	theme	presented	1573:1581	arg1	sensor					1583:1588	the presented sensor	1569:1588	the presented sensor	1569:1588	In addition, validation of the applicability of the presented sensor was also performed for the determination of three phenols in tap water sample with satisfactory results.
27415597	2	3	theme	coupled	411:417	arg1	cMWCNT					458:463	cMWCNT	458:463	cMWCNT	458:463	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	2	3	theme	coupled	411:417	arg1	nanotube					448:455	coupled carboxyl-multi-walled carbon nanotube	411:455	coupled carboxyl-multi-walled carbon nanotube (cMWCNT)	411:464	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	0	4	theme	edge-functionalized	103:121	arg1	composite					132:140	coupled cMWCNT/cyclodextrin edge-functionalized graphene composite	75:140	coupled cMWCNT/cyclodextrin edge-functionalized graphene composite	75:140	Highly-sensitive electrocatalytic determination for toxic phenols based on coupled cMWCNT/cyclodextrin edge-functionalized graphene composite.
27415597	8	5	theme	tap	1651:1653	arg1	sample					1661:1666	tap water sample	1651:1666	tap water sample with satisfactory results	1651:1692	In addition, validation of the applicability of the presented sensor was also performed for the determination of three phenols in tap water sample with satisfactory results.
27415597	4	6	theme	sensing	995:1001	arg1	architecture					1003:1014	the 3D simultaneously reduced and self-assembled sensing architecture	946:1014	the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT)	946:1029	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	4	6	theme	sensing	995:1001	arg1	GN-CD-cMWCNT					1017:1028	GN-CD-cMWCNT	1017:1028	GN-CD-cMWCNT	1017:1028	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	2	7	theme	electrochemical	379:393	arg1	sensor					395:400	a rapid and sensitive electrochemical sensor	357:400	a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite	357:525	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	7	8	theme	metal	1509:1513	arg1	ions					1515:1518	metal ions	1509:1518	metal ions	1509:1518	And, GN-CD-cMWCNT shows an excellent anti-interference ability against electroactive species and metal ions.
27415597	1	9	from	significance	223:234	arg1	monitoring					253:262	environmental monitoring	239:262	environmental monitoring due to their low degradation and high toxicity to the environment	239:328	Highly-sensitive electrocatalytic determination of toxic phenol compounds is of significance in environmental monitoring due to their low degradation and high toxicity to the environment and humans.
27415597	1	9	from	significance	223:234	arg1	humans					334:339	humans	334:339	humans	334:339	Highly-sensitive electrocatalytic determination of toxic phenol compounds is of significance in environmental monitoring due to their low degradation and high toxicity to the environment and humans.
27415597	8	10	with	sample	1661:1666	arg1	results					1686:1692	satisfactory results	1673:1692	satisfactory results	1673:1692	In addition, validation of the applicability of the presented sensor was also performed for the determination of three phenols in tap water sample with satisfactory results.
27415597	8	11	from	determination	1617:1629	arg1	sample					1661:1666	tap water sample	1651:1666	tap water sample with satisfactory results	1651:1692	In addition, validation of the applicability of the presented sensor was also performed for the determination of three phenols in tap water sample with satisfactory results.
27415597	3	12	from	microscope	714:723	arg1	studies					683:689	The morphology studies	668:689	The morphology studies from scanning electron microscope and transmission electron microscope analysis	668:769	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	6	13	theme	4-AP	1260:1263	arg1	linear					1284:1289	linear	1284:1289	linear	1284:1289	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	6	13	theme	4-AP	1260:1263	arg1	responses					1247:1255	the current responses	1235:1255	the current responses of 4-AP, 4-CP and 4-NP	1235:1278	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	2	14	theme	sensitive	369:377	arg1	sensor					395:400	a rapid and sensitive electrochemical sensor	357:400	a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite	357:525	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	2	15	theme	phenols	594:600	arg1	detection					567:575	trace detection	561:575	trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP)	561:665	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	8	16	theme	water	1655:1659	arg1	sample					1661:1666	tap water sample	1651:1666	tap water sample with satisfactory results	1651:1692	In addition, validation of the applicability of the presented sensor was also performed for the determination of three phenols in tap water sample with satisfactory results.
27415597	4	17	theme	reduced	968:974	arg1	architecture					1003:1014	the 3D simultaneously reduced and self-assembled sensing architecture	946:1014	the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT)	946:1029	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	4	17	theme	reduced	968:974	arg1	GN-CD-cMWCNT					1017:1028	GN-CD-cMWCNT	1017:1028	GN-CD-cMWCNT	1017:1028	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	6	18	theme	optimal	1215:1221	arg1	conditions					1223:1232	optimal conditions	1215:1232	optimal conditions	1215:1232	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	7	19	theme	electroactive	1483:1495	arg1	species					1497:1503	electroactive species	1483:1503	electroactive species	1483:1503	And, GN-CD-cMWCNT shows an excellent anti-interference ability against electroactive species and metal ions.
27415597	2	20	theme	typical	586:592	arg1	phenols					594:600	three typical phenols	580:600	three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP)	580:665	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	2	20	theme	typical	586:592	arg1	4-aminophenol					603:615	4-aminophenol	603:615	4-aminophenol	603:615	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	3	21	theme	conductive	796:805	arg1	bridges					807:813	conductive bridges	796:813	conductive bridges	796:813	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	6	22	theme	4-NP	1275:1278	arg1	linear					1284:1289	linear	1284:1289	linear	1284:1289	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	6	22	theme	4-NP	1275:1278	arg1	responses					1247:1255	the current responses	1235:1255	the current responses of 4-AP, 4-CP and 4-NP	1235:1278	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	0	23	theme	Highly-sensitive	0:15	arg1	determination					34:46	Highly-sensitive electrocatalytic determination	0:46	Highly-sensitive electrocatalytic determination for toxic phenols	0:64	Highly-sensitive electrocatalytic determination for toxic phenols based on coupled cMWCNT/cyclodextrin edge-functionalized graphene composite.
27415597	1	24	theme	environmental	239:251	arg1	monitoring					253:262	environmental monitoring	239:262	environmental monitoring due to their low degradation and high toxicity to the environment	239:328	Highly-sensitive electrocatalytic determination of toxic phenol compounds is of significance in environmental monitoring due to their low degradation and high toxicity to the environment and humans.
27415597	0	25	theme	electrocatalytic	17:32	arg1	determination					34:46	Highly-sensitive electrocatalytic determination	0:46	Highly-sensitive electrocatalytic determination for toxic phenols	0:64	Highly-sensitive electrocatalytic determination for toxic phenols based on coupled cMWCNT/cyclodextrin edge-functionalized graphene composite.
27415597	2	26	theme	trace	561:565	arg1	detection					567:575	trace detection	561:575	trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP)	561:665	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	4	27	theme	self-assembled	980:993	arg1	architecture					1003:1014	the 3D simultaneously reduced and self-assembled sensing architecture	946:1014	the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT)	946:1029	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	4	27	theme	self-assembled	980:993	arg1	GN-CD-cMWCNT					1017:1028	GN-CD-cMWCNT	1017:1028	GN-CD-cMWCNT	1017:1028	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	6	28	with	ranges	1328:1333	arg1	limit					1355:1359	low detection limit	1341:1359	low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively	1341:1409	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	7	29	theme	excellent	1439:1447	arg1	ability					1467:1473	an excellent anti-interference ability	1436:1473	an excellent anti-interference ability against electroactive species and metal ions	1436:1518	And, GN-CD-cMWCNT shows an excellent anti-interference ability against electroactive species and metal ions.
27415597	2	30	theme	graphene	508:515	arg1	composite					517:525	cyclodextrin (CD) edge-functionalized graphene composite	470:525	cyclodextrin (CD) edge-functionalized graphene composite	470:525	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	3	31	theme	CD	851:852	arg1	layers					883:888	CD edge-functionalized graphene layers	851:888	CD edge-functionalized graphene layers	851:888	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	6	32	theme	0.027μM	1381:1387	arg1	limit					1355:1359	low detection limit	1341:1359	low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively	1341:1409	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	2	33	theme	rapid	359:363	arg1	sensor					395:400	a rapid and sensitive electrochemical sensor	357:400	a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite	357:525	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	8	34	theme	applicability	1552:1564	arg1	validation					1534:1543	validation	1534:1543	validation of the applicability of the presented sensor	1534:1588	In addition, validation of the applicability of the presented sensor was also performed for the determination of three phenols in tap water sample with satisfactory results.
27415597	7	35	theme	anti-interference	1449:1465	arg1	ability					1467:1473	an excellent anti-interference ability	1436:1473	an excellent anti-interference ability against electroactive species and metal ions	1436:1518	And, GN-CD-cMWCNT shows an excellent anti-interference ability against electroactive species and metal ions.
27415597	2	36	theme	edge-functionalized	488:506	arg1	composite					517:525	cyclodextrin (CD) edge-functionalized graphene composite	470:525	cyclodextrin (CD) edge-functionalized graphene composite	470:525	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	3	37	theme	edge-functionalized	854:872	arg1	layers					883:888	CD edge-functionalized graphene layers	851:888	CD edge-functionalized graphene layers	851:888	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	4	38	theme	CDs	1053:1055	arg1	CDs					1053:1055	CDs	1053:1055	CDs	1053:1055	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	4	38	theme	CDs	1053:1055	arg1	amounts					1042:1048	trace amounts	1036:1048	trace amounts of CDs	1036:1055	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	1	39	theme	Highly-sensitive	143:158	arg1	determination					177:189	Highly-sensitive electrocatalytic determination	143:189	Highly-sensitive electrocatalytic determination of toxic phenol compounds	143:215	Highly-sensitive electrocatalytic determination of toxic phenol compounds is of significance in environmental monitoring due to their low degradation and high toxicity to the environment and humans.
27415597	3	40	from	analysis	762:769	arg1	studies					683:689	The morphology studies	668:689	The morphology studies from scanning electron microscope and transmission electron microscope analysis	668:769	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	1	41	theme	electrocatalytic	160:175	arg1	determination					177:189	Highly-sensitive electrocatalytic determination	143:189	Highly-sensitive electrocatalytic determination of toxic phenol compounds	143:215	Highly-sensitive electrocatalytic determination of toxic phenol compounds is of significance in environmental monitoring due to their low degradation and high toxicity to the environment and humans.
27415597	6	42	theme	detection	1345:1353	arg1	limit					1355:1359	low detection limit	1341:1359	low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively	1341:1409	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	1	43	theme	compounds	207:215	arg1	determination					177:189	Highly-sensitive electrocatalytic determination	143:189	Highly-sensitive electrocatalytic determination of toxic phenol compounds	143:215	Highly-sensitive electrocatalytic determination of toxic phenol compounds is of significance in environmental monitoring due to their low degradation and high toxicity to the environment and humans.
27415597	2	44	dep	4-aminophenol	603:615	arg1	4-CP					640:643	4-CP	640:643	4-CP	640:643	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	2	44	dep	4-aminophenol	603:615	arg1	4-chlorophenol					624:637	4-chlorophenol	624:637	4-chlorophenol	624:637	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	2	44	dep	4-aminophenol	603:615	arg1	4-NP					661:664	4-NP	661:664	4-NP	661:664	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	2	44	dep	4-aminophenol	603:615	arg1	4-nitrophenol					646:658	4-nitrophenol	646:658	4-nitrophenol	646:658	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	2	44	dep	4-aminophenol	603:615	arg1	4-AP					618:621	4-AP	618:621	4-AP	618:621	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	8	45	theme	sensor	1583:1588	arg1	applicability					1552:1564	the applicability	1548:1564	the applicability of the presented sensor	1548:1588	In addition, validation of the applicability of the presented sensor was also performed for the determination of three phenols in tap water sample with satisfactory results.
27415597	5	46	theme	high	1177:1180	arg1	sensitivity					1182:1192	high sensitivity	1177:1192	high sensitivity	1177:1192	The electrochemical studies demonstrated that GN-CD-cMWCNT displays excellent electrocatalytic activity, high sensitivity and stability.
27415597	6	47	theme	low	1341:1343	arg1	limit					1355:1359	low detection limit	1341:1359	low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively	1341:1409	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	1	48	theme	low	277:279	arg1	degradation					281:291	their low degradation	271:291	their low degradation	271:291	Highly-sensitive electrocatalytic determination of toxic phenol compounds is of significance in environmental monitoring due to their low degradation and high toxicity to the environment and humans.
27415597	0	49	theme	toxic	52:56	arg1	phenols					58:64	toxic phenols	52:64	toxic phenols	52:64	Highly-sensitive electrocatalytic determination for toxic phenols based on coupled cMWCNT/cyclodextrin edge-functionalized graphene composite.
27415597	3	50	theme	scanning	696:703	arg1	microscope					714:723	scanning electron microscope	696:723	scanning electron microscope	696:723	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	6	51	theme	0.017	1371:1375	arg1	limit					1355:1359	low detection limit	1341:1359	low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively	1341:1409	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	8	52	from	sample	1661:1666	arg1	determination					1617:1629	the determination	1613:1629	the determination of three phenols in tap water sample with satisfactory results	1613:1692	In addition, validation of the applicability of the presented sensor was also performed for the determination of three phenols in tap water sample with satisfactory results.
27415597	3	53	theme	electron	705:712	arg1	microscope					714:723	scanning electron microscope	696:723	scanning electron microscope	696:723	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	4	54	theme	3D	950:951	arg1	architecture					1003:1014	the 3D simultaneously reduced and self-assembled sensing architecture	946:1014	the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT)	946:1029	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	4	54	theme	3D	950:951	arg1	GN-CD-cMWCNT					1017:1028	GN-CD-cMWCNT	1017:1028	GN-CD-cMWCNT	1017:1028	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	6	55	theme	4-CP	1266:1269	arg1	linear					1284:1289	linear	1284:1289	linear	1284:1289	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	6	55	theme	4-CP	1266:1269	arg1	responses					1247:1255	the current responses	1235:1255	the current responses of 4-AP, 4-CP and 4-NP	1235:1278	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	8	56	theme	satisfactory	1673:1684	arg1	results					1686:1692	satisfactory results	1673:1692	satisfactory results	1673:1692	In addition, validation of the applicability of the presented sensor was also performed for the determination of three phenols in tap water sample with satisfactory results.
27415597	3	57	theme	graphene	874:881	arg1	layers					883:888	CD edge-functionalized graphene layers	851:888	CD edge-functionalized graphene layers	851:888	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	3	58	theme	microscope	751:760	arg1	analysis					762:769	transmission electron microscope analysis	729:769	transmission electron microscope analysis	729:769	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	6	59	theme	0.019	1364:1368	arg1	limit					1355:1359	low detection limit	1341:1359	low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively	1341:1409	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	4	60	theme	electrocatalytic	904:919	arg1	performance					931:941	The electrocatalytic detection performance	900:941	The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs	900:1055	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	5	61	theme	excellent	1140:1148	arg1	activity					1167:1174	excellent electrocatalytic activity	1140:1174	excellent electrocatalytic activity	1140:1174	The electrochemical studies demonstrated that GN-CD-cMWCNT displays excellent electrocatalytic activity, high sensitivity and stability.
27415597	1	62	theme	toxic	194:198	arg1	compounds					207:215	toxic phenol compounds	194:215	toxic phenol compounds	194:215	Highly-sensitive electrocatalytic determination of toxic phenol compounds is of significance in environmental monitoring due to their low degradation and high toxicity to the environment and humans.
27415597	5	63	theme	electrochemical	1076:1090	arg1	studies					1092:1098	The electrochemical studies	1072:1098	The electrochemical studies	1072:1098	The electrochemical studies demonstrated that GN-CD-cMWCNT displays excellent electrocatalytic activity, high sensitivity and stability.
27415597	4	64	theme	trace	1036:1040	arg1	CDs					1053:1055	CDs	1053:1055	CDs	1053:1055	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	4	64	theme	trace	1036:1040	arg1	amounts					1042:1048	trace amounts	1036:1048	trace amounts of CDs	1036:1055	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	3	65	theme	transmission	729:740	arg1	microscope					751:760	transmission electron microscope	729:760	transmission electron microscope analysis	729:769	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	5	66	theme	electrocatalytic	1150:1165	arg1	activity					1167:1174	excellent electrocatalytic activity	1140:1174	excellent electrocatalytic activity	1140:1174	The electrochemical studies demonstrated that GN-CD-cMWCNT displays excellent electrocatalytic activity, high sensitivity and stability.
27415597	8	67	from	phenols	1640:1646	arg1	sample					1661:1666	tap water sample	1651:1666	tap water sample with satisfactory results	1651:1692	In addition, validation of the applicability of the presented sensor was also performed for the determination of three phenols in tap water sample with satisfactory results.
27415597	1	68	theme	phenol	200:205	arg1	compounds					207:215	toxic phenol compounds	194:215	toxic phenol compounds	194:215	Highly-sensitive electrocatalytic determination of toxic phenol compounds is of significance in environmental monitoring due to their low degradation and high toxicity to the environment and humans.
27415597	1	69	theme	high	297:300	arg1	toxicity					302:309	high toxicity	297:309	high toxicity	297:309	Highly-sensitive electrocatalytic determination of toxic phenol compounds is of significance in environmental monitoring due to their low degradation and high toxicity to the environment and humans.
27415597	0	70	theme	cMWCNT/cyclodextrin	83:101	arg1	composite					132:140	coupled cMWCNT/cyclodextrin edge-functionalized graphene composite	75:140	coupled cMWCNT/cyclodextrin edge-functionalized graphene composite	75:140	Highly-sensitive electrocatalytic determination for toxic phenols based on coupled cMWCNT/cyclodextrin edge-functionalized graphene composite.
27415597	3	71	theme	electron	742:749	arg1	microscope					751:760	transmission electron microscope	729:760	transmission electron microscope analysis	729:769	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	4	72	theme	detection	921:929	arg1	performance					931:941	The electrocatalytic detection performance	900:941	The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs	900:1055	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	6	73	theme	different	1318:1326	arg1	ranges					1328:1333	two different ranges	1314:1333	two different ranges	1314:1333	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	2	74	theme	carbon	441:446	arg1	cMWCNT					458:463	cMWCNT	458:463	cMWCNT	458:463	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	2	74	theme	carbon	441:446	arg1	nanotube					448:455	coupled carboxyl-multi-walled carbon nanotube	411:455	coupled carboxyl-multi-walled carbon nanotube (cMWCNT)	411:464	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	6	75	theme	current	1239:1245	arg1	linear					1284:1289	linear	1284:1289	linear	1284:1289	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	6	75	theme	current	1239:1245	arg1	responses					1247:1255	the current responses	1235:1255	the current responses of 4-AP, 4-CP and 4-NP	1235:1278	Under optimal conditions, the current responses of 4-AP, 4-CP and 4-NP are linear to concentrations over two different ranges, with low detection limit of 0.019, 0.017 and 0.027μM (S/N=3), respectively.
27415597	0	76	theme	coupled	75:81	arg1	composite					132:140	coupled cMWCNT/cyclodextrin edge-functionalized graphene composite	75:140	coupled cMWCNT/cyclodextrin edge-functionalized graphene composite	75:140	Highly-sensitive electrocatalytic determination for toxic phenols based on coupled cMWCNT/cyclodextrin edge-functionalized graphene composite.
27415597	8	77	theme	phenols	1640:1646	arg1	determination					1617:1629	the determination	1613:1629	the determination of three phenols in tap water sample with satisfactory results	1613:1692	In addition, validation of the applicability of the presented sensor was also performed for the determination of three phenols in tap water sample with satisfactory results.
27415597	3	78	theme	morphology	672:681	arg1	studies					683:689	The morphology studies	668:689	The morphology studies from scanning electron microscope and transmission electron microscope analysis	668:769	The morphology studies from scanning electron microscope and transmission electron microscope analysis revealed that cMWCNTs as conductive bridges were successfully incorporated into CD edge-functionalized graphene layers.
27415597	2	79	theme	carboxyl-multi-walled	419:439	arg1	cMWCNT					458:463	cMWCNT	458:463	cMWCNT	458:463	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	2	79	theme	carboxyl-multi-walled	419:439	arg1	nanotube					448:455	coupled carboxyl-multi-walled carbon nanotube	411:455	coupled carboxyl-multi-walled carbon nanotube (cMWCNT)	411:464	In this paper, a rapid and sensitive electrochemical sensor based on coupled carboxyl-multi-walled carbon nanotube (cMWCNT) and cyclodextrin (CD) edge-functionalized graphene composite was successfully employed towards trace detection of three typical phenols (4-aminophenol, 4-AP; 4-chlorophenol, 4-CP; 4-nitrophenol, 4-NP).
27415597	4	80	with	performance	931:941	arg1	CDs					1053:1055	CDs	1053:1055	CDs	1053:1055	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
27415597	4	80	with	performance	931:941	arg1	amounts					1042:1048	trace amounts	1036:1048	trace amounts of CDs	1036:1055	Further, The electrocatalytic detection performance of the 3D simultaneously reduced and self-assembled sensing architecture (GN-CD-cMWCNT) with trace amounts of CDs was evaluated.
25219460	7	0	theme	total	1283:1287	arg1	motility					1289:1296	total motility	1283:1296	total motility (only with 1.5% lecithin)	1283:1322	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	7	0	theme	total	1283:1287	arg1	lecithin					1314:1321	only with 1.5% lecithin	1299:1321	only with 1.5% lecithin	1299:1321	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	6	1	theme	GSH-PX	1108:1113	arg1	activity					1115:1122	GSH-PX activity	1108:1122	GSH-PX activity	1108:1122	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	6	1	theme	GSH-PX	1108:1113	arg1	parameters					1066:1075	Linearity-related parameters	1048:1075	Linearity-related parameters	1048:1075	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	7	2	dep	lecithin	1358:1365	arg1	both					1368:1371	both	1368:1371	both	1368:1371	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	9	3	theme	positive	1619:1626	arg1	effect					1628:1633	a positive effect	1617:1633	a positive effect	1617:1633	Increasing the lecithin content could have a positive effect, but further studies are needed.
25219460	2	4	contain	containing	321:330	arg1	extender					312:319	an extender	309:319	an extender containing soybean lecithin for freezing ram semen	309:370	HA has not been tested yet in an extender containing soybean lecithin for freezing ram semen.
25219460	2	4	contain	containing	321:330	arg2	lecithin					340:347	soybean lecithin	332:347	soybean lecithin	332:347	HA has not been tested yet in an extender containing soybean lecithin for freezing ram semen.
25219460	4	5	theme	breeding	749:756	arg1	season					758:763	the breeding season	745:763	the breeding season	745:763	Semen was collected from four Mehraban rams during the breeding season and frozen in the six lecithin×HA extenders.
25219460	1	6	theme	hyaluronic	177:186	arg1	HA					194:195	HA	194:195	HA	194:195	A soybean lecithin-based extender supplemented with hyaluronic acid (HA) was assayed for effectiveness to improve the quality of frozen-thawed ram semen.
25219460	1	6	theme	hyaluronic	177:186	arg1	acid					188:191	hyaluronic acid	177:191	hyaluronic acid (HA)	177:196	A soybean lecithin-based extender supplemented with hyaluronic acid (HA) was assayed for effectiveness to improve the quality of frozen-thawed ram semen.
25219460	1	7	theme	frozen-thawed	254:266	arg1	semen					272:276	frozen-thawed ram semen	254:276	frozen-thawed ram semen	254:276	A soybean lecithin-based extender supplemented with hyaluronic acid (HA) was assayed for effectiveness to improve the quality of frozen-thawed ram semen.
25219460	6	8	theme	extender	1182:1189	arg1	composition					1191:1201	the extender composition	1178:1201	the extender composition	1178:1201	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	0	9	from	Cryopreservation	0:15	arg1	extenders					33:41	extenders	33:41	extenders	33:41	Cryopreservation of ram semen in extenders containing soybean lecithin as cryoprotectant and hyaluronic acid as antioxidant.
25219460	8	10	theme	quality-related	1534:1548	arg1	variables					1550:1558	quality-related variables	1534:1558	quality-related variables in ram semen	1534:1571	In conclusion, adding HA to the freezing extender supplemented with soybean lecithin failed to improve quality-related variables in ram semen.
25219460	3	11	from	0	479:479	arg1	%					452:452	1%	451:452	1%	451:452	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	11	from	0	479:479	arg1	%					460:460	1.5%	457:460	1.5%	457:460	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	1	12	theme	ram	268:270	arg1	semen					272:276	frozen-thawed ram semen	254:276	frozen-thawed ram semen	254:276	A soybean lecithin-based extender supplemented with hyaluronic acid (HA) was assayed for effectiveness to improve the quality of frozen-thawed ram semen.
25219460	0	13	theme	hyaluronic	93:102	arg1	acid					104:107	hyaluronic acid	93:107	hyaluronic acid	93:107	Cryopreservation of ram semen in extenders containing soybean lecithin as cryoprotectant and hyaluronic acid as antioxidant.
25219460	3	14	from	effects	420:426	arg1	%					460:460	1.5%	457:460	1.5%	457:460	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	14	from	effects	420:426	arg1	peroxidation					636:647	lipid peroxidation	630:647	lipid peroxidation	630:647	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	14	from	effects	420:426	arg1	characteristics					541:555	the motion characteristics	530:555	the motion characteristics	530:555	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	14	from	effects	420:426	arg1	status					663:668	acrosomal status	653:668	acrosomal status	653:668	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	14	from	effects	420:426	arg1	viability					585:593	viability	585:593	viability	585:593	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	14	from	effects	420:426	arg1	%					452:452	1%	451:452	1%	451:452	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	14	from	effects	420:426	arg1	extender					518:525	a Tris-based extender	505:525	a Tris-based extender	505:525	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	14	from	effects	420:426	arg1	activity					620:627	GSH peroxidase (GSH-PX) activity	596:627	GSH peroxidase (GSH-PX) activity	596:627	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	14	from	effects	420:426	arg1	integrity					567:575	membrane integrity	558:575	membrane integrity (HOST)	558:582	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	15	from	%	460:460	arg1	effects					420:426	the effects	416:426	the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status	416:668	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	15	from	%	460:460	arg1	lecithin					439:446	soybean lecithin	431:446	soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender	431:525	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	8	16	theme	freezing	1463:1470	arg1	extender					1472:1479	the freezing extender	1459:1479	the freezing extender supplemented with soybean lecithin	1459:1514	In conclusion, adding HA to the freezing extender supplemented with soybean lecithin failed to improve quality-related variables in ram semen.
25219460	7	17	theme	1 mg ml	1336:1342	arg1	HA					1348:1349	1 mg ml(-1) HA	1336:1349	1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin	1336:1404	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	1	18	theme	semen	272:276	arg1	quality					243:249	the quality	239:249	the quality of frozen-thawed ram semen	239:276	A soybean lecithin-based extender supplemented with hyaluronic acid (HA) was assayed for effectiveness to improve the quality of frozen-thawed ram semen.
25219460	5	19	theme	membrane	941:948	arg1	integrity					950:958	membrane integrity	941:958	membrane integrity (44.5%±1.7)	941:970	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	5	19	theme	membrane	941:948	arg1	%					965:965	44.5%±1.7	961:969	44.5%±1.7	961:969	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	9	20	theme	further	1640:1646	arg1	studies					1648:1654	further studies	1640:1654	further studies	1640:1654	Increasing the lecithin content could have a positive effect, but further studies are needed.
25219460	0	21	from	extenders	33:41	arg1	Cryopreservation					0:15	Cryopreservation	0:15	Cryopreservation of ram semen in extenders	0:41	Cryopreservation of ram semen in extenders containing soybean lecithin as cryoprotectant and hyaluronic acid as antioxidant.
25219460	4	22	theme	Mehraban	724:731	arg1	rams					733:736	four Mehraban rams	719:736	four Mehraban rams	719:736	Semen was collected from four Mehraban rams during the breeding season and frozen in the six lecithin×HA extenders.
25219460	3	23	from	lecithin	439:446	arg1	extender					518:525	a Tris-based extender	505:525	a Tris-based extender	505:525	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	7	24	theme	sperm	1250:1254	arg1	velocity					1256:1263	sperm velocity	1250:1263	sperm velocity (1 mg ml(-1) HA)	1250:1280	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	7	24	theme	sperm	1250:1254	arg1	HA					1278:1279	1 mg ml(-1) HA	1266:1279	1 mg ml(-1) HA	1266:1279	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	3	25	from	extender	518:525	arg1	effects					420:426	the effects	416:426	the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status	416:668	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	26	theme	peroxidase	600:609	arg1	characteristics					541:555	the motion characteristics	530:555	the motion characteristics	530:555	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	26	theme	peroxidase	600:609	arg1	activity					620:627	GSH peroxidase (GSH-PX) activity	596:627	GSH peroxidase (GSH-PX) activity	596:627	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	8	27	from	variables	1550:1558	arg1	semen					1567:1571	ram semen	1563:1571	ram semen	1563:1571	In conclusion, adding HA to the freezing extender supplemented with soybean lecithin failed to improve quality-related variables in ram semen.
25219460	7	28	theme	membrane	1411:1418	arg1	integrity					1420:1428	membrane integrity	1411:1428	membrane integrity	1411:1428	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	3	29	theme	study	395:399	arg1	aim					383:385	the aim	379:385	the aim of this study	379:399	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	7	30	dep	%	1312:1312	arg1	1.5					1309:1311	1.5	1309:1311	1.5	1309:1311	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	5	31	theme	1.5	834:836	arg1	%					837:837	%	837:837	%	837:837	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	7	32	theme	1 mg ml	1266:1272	arg1	velocity					1256:1263	sperm velocity	1250:1263	sperm velocity (1 mg ml(-1) HA)	1250:1280	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	7	32	theme	1 mg ml	1266:1272	arg1	HA					1278:1279	1 mg ml(-1) HA	1266:1279	1 mg ml(-1) HA	1266:1279	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	0	33	theme	semen	24:28	arg1	Cryopreservation					0:15	Cryopreservation	0:15	Cryopreservation of ram semen in extenders	0:41	Cryopreservation of ram semen in extenders containing soybean lecithin as cryoprotectant and hyaluronic acid as antioxidant.
25219460	3	34	theme	lipid	630:634	arg1	characteristics					541:555	the motion characteristics	530:555	the motion characteristics	530:555	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	34	theme	lipid	630:634	arg1	peroxidation					636:647	lipid peroxidation	630:647	lipid peroxidation	630:647	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	35	from	%	452:452	arg1	effects					420:426	the effects	416:426	the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status	416:668	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	35	from	%	452:452	arg1	lecithin					439:446	soybean lecithin	431:446	soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender	431:525	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	5	36	theme	%	837:837	arg1	lecithin					839:846	1.5% lecithin	834:846	1.5% lecithin supplemented with no HA	834:870	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	0	37	theme	ram	20:22	arg1	semen					24:28	ram semen	20:28	ram semen in extenders	20:41	Cryopreservation of ram semen in extenders containing soybean lecithin as cryoprotectant and hyaluronic acid as antioxidant.
25219460	5	38	theme	lecithin	996:1003	arg1	concentration					1005:1017	the lecithin concentration	992:1017	the lecithin concentration	992:1017	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	9	39	contain	have	1612:1615	arg2	effect					1628:1633	a positive effect	1617:1633	a positive effect	1617:1633	Increasing the lecithin content could have a positive effect, but further studies are needed.
25219460	9	39	contain	have	1612:1615	arg1	content					1598:1604	the lecithin content	1585:1604	Increasing the lecithin content	1574:1604	Increasing the lecithin content could have a positive effect, but further studies are needed.
25219460	7	40	theme	%	1395:1395	arg1	lecithin					1397:1404	1.5% lecithin	1392:1404	1.5% lecithin	1392:1404	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	7	41	theme	1.5	1392:1394	arg1	%					1395:1395	%	1395:1395	%	1395:1395	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	3	42	theme	soybean	431:437	arg1	lecithin					439:446	soybean lecithin	431:446	soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender	431:525	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	5	43	theme	concentration	1005:1017	arg1	effects					981:987	the effects	977:987	the effects of the lecithin concentration	977:1017	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	5	44	contain	containing	823:832	arg2	lecithin					839:846	1.5% lecithin	834:846	1.5% lecithin supplemented with no HA	834:870	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	5	44	contain	containing	823:832	arg1	extender					814:821	The extender	810:821	The extender containing 1.5% lecithin supplemented with no HA	810:870	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	3	45	theme	acrosomal	653:661	arg1	characteristics					541:555	the motion characteristics	530:555	the motion characteristics	530:555	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	45	theme	acrosomal	653:661	arg1	status					663:668	acrosomal status	653:668	acrosomal status	653:668	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	6	46	theme	acrosomal	1140:1148	arg1	status					1150:1155	acrosomal status	1140:1155	acrosomal status	1140:1155	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	6	46	theme	acrosomal	1140:1148	arg1	parameters					1066:1075	Linearity-related parameters	1048:1075	Linearity-related parameters	1048:1075	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	0	47	theme	soybean	54:60	arg1	lecithin					62:69	soybean lecithin	54:69	soybean lecithin	54:69	Cryopreservation of ram semen in extenders containing soybean lecithin as cryoprotectant and hyaluronic acid as antioxidant.
25219460	7	48	theme	%	1356:1356	arg1	lecithin					1358:1365	1% lecithin	1355:1365	1% lecithin	1355:1365	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	3	49	theme	motion	534:539	arg1	peroxidation					636:647	lipid peroxidation	630:647	lipid peroxidation	630:647	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	49	theme	motion	534:539	arg1	characteristics					541:555	the motion characteristics	530:555	the motion characteristics	530:555	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	49	theme	motion	534:539	arg1	status					663:668	acrosomal status	653:668	acrosomal status	653:668	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	49	theme	motion	534:539	arg1	viability					585:593	viability	585:593	viability	585:593	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	49	theme	motion	534:539	arg1	activity					620:627	GSH peroxidase (GSH-PX) activity	596:627	GSH peroxidase (GSH-PX) activity	596:627	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	49	theme	motion	534:539	arg1	integrity					567:575	membrane integrity	558:575	membrane integrity (HOST)	558:582	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	6	50	theme	Linearity-related	1048:1064	arg1	status					1150:1155	acrosomal status	1140:1155	acrosomal status	1140:1155	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	6	50	theme	Linearity-related	1048:1064	arg1	ALH					1078:1080	ALH	1078:1080	ALH	1078:1080	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	6	50	theme	Linearity-related	1048:1064	arg1	BCF					1083:1085	BCF	1083:1085	BCF	1083:1085	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	6	50	theme	Linearity-related	1048:1064	arg1	activity					1115:1122	GSH-PX activity	1108:1122	GSH-PX activity	1108:1122	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	6	50	theme	Linearity-related	1048:1064	arg1	morphology					1125:1134	morphology	1125:1134	morphology	1125:1134	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	6	50	theme	Linearity-related	1048:1064	arg1	parameters					1066:1075	Linearity-related parameters	1048:1075	Linearity-related parameters	1048:1075	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	6	50	theme	Linearity-related	1048:1064	arg1	peroxidation					1094:1105	lipid peroxidation	1088:1105	lipid peroxidation	1088:1105	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	3	51	theme	GSH	596:598	arg1	peroxidase					600:609	GSH peroxidase	596:609	GSH peroxidase (GSH-PX) activity	596:627	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	51	theme	GSH	596:598	arg1	GSH-PX					612:617	GSH-PX	612:617	GSH-PX	612:617	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	2	52	theme	soybean	332:338	arg1	lecithin					340:347	soybean lecithin	332:347	soybean lecithin	332:347	HA has not been tested yet in an extender containing soybean lecithin for freezing ram semen.
25219460	6	53	theme	lipid	1088:1092	arg1	peroxidation					1094:1105	lipid peroxidation	1088:1105	lipid peroxidation	1088:1105	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	6	53	theme	lipid	1088:1092	arg1	parameters					1066:1075	Linearity-related parameters	1048:1075	Linearity-related parameters	1048:1075	Linearity-related parameters, ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status were not affected by the extender composition.
25219460	8	54	theme	soybean	1499:1505	arg1	lecithin					1507:1514	soybean lecithin	1499:1514	soybean lecithin	1499:1514	In conclusion, adding HA to the freezing extender supplemented with soybean lecithin failed to improve quality-related variables in ram semen.
25219460	9	55	theme	lecithin	1589:1596	arg1	content					1598:1604	the lecithin content	1585:1604	Increasing the lecithin content	1574:1604	Increasing the lecithin content could have a positive effect, but further studies are needed.
25219460	7	56	theme	1	1355:1355	arg1	%					1356:1356	%	1356:1356	%	1356:1356	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	4	57	theme	lecithin×HA	787:797	arg1	extenders					799:807	the six lecithin×HA extenders	779:807	the six lecithin×HA extenders	779:807	Semen was collected from four Mehraban rams during the breeding season and frozen in the six lecithin×HA extenders.
25219460	7	58	dep	viability	1325:1333	arg1	HA					1348:1349	1 mg ml(-1) HA	1336:1349	1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin	1336:1404	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	1	59	theme	soybean	127:133	arg1	extender					150:157	A soybean lecithin-based extender	125:157	A soybean lecithin-based extender supplemented with hyaluronic acid (HA)	125:196	A soybean lecithin-based extender supplemented with hyaluronic acid (HA) was assayed for effectiveness to improve the quality of frozen-thawed ram semen.
25219460	3	60	from	0.5	482:484	arg1	%					452:452	1%	451:452	1%	451:452	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	60	from	0.5	482:484	arg1	%					460:460	1.5%	457:460	1.5%	457:460	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	8	61	theme	ram	1563:1565	arg1	semen					1567:1571	ram semen	1563:1571	ram semen	1563:1571	In conclusion, adding HA to the freezing extender supplemented with soybean lecithin failed to improve quality-related variables in ram semen.
25219460	3	62	theme	membrane	558:565	arg1	characteristics					541:555	the motion characteristics	530:555	the motion characteristics	530:555	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	62	theme	membrane	558:565	arg1	integrity					567:575	membrane integrity	558:575	membrane integrity (HOST)	558:582	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	62	theme	membrane	558:565	arg1	HOST					578:581	HOST	578:581	HOST	578:581	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	5	63	theme	higher	880:885	arg1	motility					893:900	higher total motility	880:900	higher total motility (52.5%±1.6)	880:912	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	5	63	theme	higher	880:885	arg1	%					907:907	52.5%±1.6	903:911	52.5%±1.6	903:911	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	1	64	theme	lecithin-based	135:148	arg1	extender					150:157	A soybean lecithin-based extender	125:157	A soybean lecithin-based extender supplemented with hyaluronic acid (HA)	125:196	A soybean lecithin-based extender supplemented with hyaluronic acid (HA) was assayed for effectiveness to improve the quality of frozen-thawed ram semen.
25219460	3	65	theme	lecithin	439:446	arg1	effects					420:426	the effects	416:426	the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status	416:668	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	5	66	theme	total	887:891	arg1	motility					893:900	higher total motility	880:900	higher total motility (52.5%±1.6)	880:912	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	5	66	theme	total	887:891	arg1	%					907:907	52.5%±1.6	903:911	52.5%±1.6	903:911	The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification.
25219460	2	67	theme	ram	362:364	arg1	semen					366:370	ram semen	362:370	ram semen	362:370	HA has not been tested yet in an extender containing soybean lecithin for freezing ram semen.
25219460	3	68	from	1 mg ml	490:496	arg1	%					452:452	1%	451:452	1%	451:452	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	3	68	from	1 mg ml	490:496	arg1	%					460:460	1.5%	457:460	1.5%	457:460	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
25219460	7	69	dep	lecithin	1314:1321	arg1	%					1312:1312	%	1312:1312	%	1312:1312	In general, adding HA significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity.
25219460	9	70	theme	Increasing	1574:1583	arg1	content					1598:1604	the lecithin content	1585:1604	Increasing the lecithin content	1574:1604	Increasing the lecithin content could have a positive effect, but further studies are needed.
25219460	0	71	from	semen	24:28	arg1	extenders					33:41	extenders	33:41	extenders	33:41	Cryopreservation of ram semen in extenders containing soybean lecithin as cryoprotectant and hyaluronic acid as antioxidant.
25219460	3	72	theme	Tris-based	507:516	arg1	extender					518:525	a Tris-based extender	505:525	a Tris-based extender	505:525	Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after freezing-thawing.
27453473	12	0	theme	100831T=CECT	1344:1355	arg1	8959T					1357:1361	=DSM 100831T=CECT 8959T	1339:1361	=DSM 100831T=CECT 8959T	1339:1361	The type strain is SD1DT(=DSM 100831T=CECT 8959T).
27453473	12	0	theme	100831T=CECT	1344:1355	arg1	SD1DT					1333:1337	SD1DT	1333:1337	SD1DT(=DSM 100831T=CECT 8959T)	1333:1362	The type strain is SD1DT(=DSM 100831T=CECT 8959T).
27453473	5	1	theme	whole	632:636	arg1	group					638:642	the whole group	628:642	the whole group of isolates	628:654	As a representative of the whole group of isolates, strain SD1DT was further characterized.
27453473	5	1	theme	whole	632:636	arg1	isolates					647:654	isolates	647:654	isolates	647:654	As a representative of the whole group of isolates, strain SD1DT was further characterized.
27453473	4	2	theme	16S	348:350	arg1	rRNA					352:355	16S rRNA	348:355	16S rRNA gene sequence comparison	348:380	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	10	3	theme	family	1232:1237	arg1	Lachnospiraceae					1239:1253	the family Lachnospiraceae	1228:1253	the family Lachnospiraceae	1228:1253	Due to the genetic and phenotypic differences to the most closely affiliated species, the isolates represent a novel species of the genus Herbinix within the family Lachnospiraceae, for which the name Herbinix luporum sp.
27453473	4	4	theme	sequence	574:581	arg1	similarity					583:592	a 16S rRNA gene sequence similarity	558:592	a 16S rRNA gene sequence similarity of 96.4 %	558:602	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	2	5	theme	Phylogenetic	117:128	arg1	studies					130:136	Phylogenetic studies	117:136	Phylogenetic studies	117:136	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	5	6	theme	group	638:642	arg1	SD1DT					664:668	strain SD1DT	657:668	strain SD1DT	657:668	As a representative of the whole group of isolates, strain SD1DT was further characterized.
27453473	5	6	theme	group	638:642	arg1	representative					610:623	a representative	608:623	a representative of the whole group of isolates	608:654	As a representative of the whole group of isolates, strain SD1DT was further characterized.
27453473	4	7	theme	gene	569:572	arg1	similarity					583:592	a 16S rRNA gene sequence similarity	558:592	a 16S rRNA gene sequence similarity of 96.4 %	558:602	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	4	8	theme	Herbinix	500:507	arg1	T3/55T					529:534	Herbinix hemicellulosilytica T3/55T	500:534	Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %	500:602	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	1	9	theme	cellulose-degrading	42:60	arg1	bacterium					62:70	a thermophilic cellulose-degrading bacterium	27:70	a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor	27:114	nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor.
27453473	8	10	theme	fatty	960:964	arg1	%					977:977	>10 %	973:977	>10 %	973:977	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	8	10	theme	fatty	960:964	arg1	C19 					985:988	C19 	985:988	C19 	985:988	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	8	10	theme	fatty	960:964	arg1	acids					966:970	The major cellular fatty acids	941:970	The major cellular fatty acids (>10 %)	941:978	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	5	11	theme	isolates	647:654	arg1	group					638:642	the whole group	628:642	the whole group of isolates	628:654	As a representative of the whole group of isolates, strain SD1DT was further characterized.
27453473	5	11	theme	isolates	647:654	arg1	isolates					647:654	isolates	647:654	isolates	647:654	As a representative of the whole group of isolates, strain SD1DT was further characterized.
27453473	8	12	theme	dimethyl	1001:1008	arg1	acetal					1010:1015	 0cyc 9,10 dimethyl acetal	990:1015	C19 : 0cyc 9,10 dimethyl acetal	985:1015	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	6	13	theme	Strain	697:702	arg1	ethanol					772:778	ethanol	772:778	ethanol	772:778	Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization.
27453473	6	13	theme	Strain	697:702	arg1	acid					789:792	butyric acid	781:792	butyric acid	781:792	Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization.
27453473	6	13	theme	Strain	697:702	arg1	SD1DT					704:708	Strain SD1DT	697:708	Strain SD1DT	697:708	Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization.
27453473	6	13	theme	Strain	697:702	arg1	acetate					763:769	acetate	763:769	acetate	763:769	Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization.
27453473	6	13	theme	Strain	697:702	arg1	hydrogen					798:805	hydrogen	798:805	hydrogen	798:805	Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization.
27453473	2	14	attach	isolated	241:248	arg2	bacteria					232:239	novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria	167:239	novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant	167:281	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	2	14	attach	isolated	241:248	arg1	plant					277:281	a thermophilic biogas plant	255:281	a thermophilic biogas plant	255:281	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	10	15	theme	Herbinix	1212:1219	arg1	species					1191:1197	a novel species	1183:1197	a novel species	1183:1197	Due to the genetic and phenotypic differences to the most closely affiliated species, the isolates represent a novel species of the genus Herbinix within the family Lachnospiraceae, for which the name Herbinix luporum sp.
27453473	6	16	theme	butyric	781:787	arg1	acid					789:792	butyric acid	781:792	butyric acid	781:792	Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization.
27453473	6	16	theme	butyric	781:787	arg1	SD1DT					704:708	Strain SD1DT	697:708	Strain SD1DT	697:708	Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization.
27453473	6	17	dep	acetate	763:769	arg1	end-products					816:827	major end-products	810:827	major end-products during fermentative cellobiose utilization	810:870	Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization.
27453473	2	18	dep	Gram-stain-positive	173:191	arg1	non-sporulating					205:219	non-sporulating	205:219	non-sporulating	205:219	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	2	18	dep	Gram-stain-positive	173:191	arg1	anaerobic					194:202	anaerobic	194:202	anaerobic	194:202	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	12	19	theme	=DSM	1339:1342	arg1	8959T					1357:1361	=DSM 100831T=CECT 8959T	1339:1361	=DSM 100831T=CECT 8959T	1339:1361	The type strain is SD1DT(=DSM 100831T=CECT 8959T).
27453473	12	19	theme	=DSM	1339:1342	arg1	SD1DT					1333:1337	SD1DT	1333:1337	SD1DT(=DSM 100831T=CECT 8959T)	1333:1362	The type strain is SD1DT(=DSM 100831T=CECT 8959T).
27453473	10	20	theme	affiliated	1140:1149	arg1	species					1151:1157	the most closely affiliated species	1123:1157	the most closely affiliated species	1123:1157	Due to the genetic and phenotypic differences to the most closely affiliated species, the isolates represent a novel species of the genus Herbinix within the family Lachnospiraceae, for which the name Herbinix luporum sp.
27453473	9	21	theme	35.1 mol	1063:1070	arg1	%					1071:1071	35.1 mol%	1063:1071	35.1 mol%	1063:1071	The DNA G+C content was 35.1 mol%.
27453473	9	21	theme	35.1 mol	1063:1070	arg1	content					1051:1057	The DNA G+C content	1039:1057	The DNA G+C content	1039:1057	The DNA G+C content was 35.1 mol%.
27453473	12	22	theme	type	1318:1321	arg1	SD1DT					1333:1337	SD1DT	1333:1337	SD1DT(=DSM 100831T=CECT 8959T)	1333:1362	The type strain is SD1DT(=DSM 100831T=CECT 8959T).
27453473	12	22	theme	type	1318:1321	arg1	strain					1323:1328	The type strain	1314:1328	The type strain	1314:1328	The type strain is SD1DT(=DSM 100831T=CECT 8959T).
27453473	8	23	dep	C19 	985:988	arg1	acetal					1010:1015	 0cyc 9,10 dimethyl acetal	990:1015	C19 : 0cyc 9,10 dimethyl acetal	985:1015	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	0	24	theme	luporum	9:15	arg1	sp					17:18	Herbinix luporum sp	0:18	Herbinix luporum sp.	0:19	Herbinix luporum sp.
27453473	4	25	theme	16S	560:562	arg1	similarity					583:592	a 16S rRNA gene sequence similarity	558:592	a 16S rRNA gene sequence similarity of 96.4 %	558:602	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	5	26	theme	strain	657:662	arg1	SD1DT					664:668	strain SD1DT	657:668	strain SD1DT	657:668	As a representative of the whole group of isolates, strain SD1DT was further characterized.
27453473	5	26	theme	strain	657:662	arg1	representative					610:623	a representative	608:623	a representative of the whole group of isolates	608:654	As a representative of the whole group of isolates, strain SD1DT was further characterized.
27453473	0	27	theme	Herbinix	0:7	arg1	sp					17:18	Herbinix luporum sp	0:18	Herbinix luporum sp.	0:19	Herbinix luporum sp.
27453473	8	28	theme	 0cyc	990:994	arg1	acetal					1010:1015	 0cyc 9,10 dimethyl acetal	990:1015	C19 : 0cyc 9,10 dimethyl acetal	985:1015	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	1	29	theme	thermophilic	88:99	arg1	reactor					108:114	a thermophilic biogas reactor	86:114	a thermophilic biogas reactor	86:114	nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor.
27453473	4	30	theme	%	447:447	arg1	identity					458:465	100 % sequence identity	443:465	100 % sequence identity	443:465	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	10	31	theme	genus	1206:1210	arg1	Herbinix					1212:1219	the genus Herbinix	1202:1219	the genus Herbinix within the family Lachnospiraceae	1202:1253	Due to the genetic and phenotypic differences to the most closely affiliated species, the isolates represent a novel species of the genus Herbinix within the family Lachnospiraceae, for which the name Herbinix luporum sp.
27453473	1	32	theme	biogas	101:106	arg1	reactor					108:114	a thermophilic biogas reactor	86:114	a thermophilic biogas reactor	86:114	nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor.
27453473	9	33	theme	DNA	1043:1045	arg1	%					1071:1071	35.1 mol%	1063:1071	35.1 mol%	1063:1071	The DNA G+C content was 35.1 mol%.
27453473	9	33	theme	DNA	1043:1045	arg1	content					1051:1057	The DNA G+C content	1039:1057	The DNA G+C content	1039:1057	The DNA G+C content was 35.1 mol%.
27453473	10	34	theme	name	1270:1273	arg1	sp					1292:1293	the name Herbinix luporum sp	1266:1293	the name Herbinix luporum sp	1266:1293	Due to the genetic and phenotypic differences to the most closely affiliated species, the isolates represent a novel species of the genus Herbinix within the family Lachnospiraceae, for which the name Herbinix luporum sp.
27453473	8	35	theme	major	945:949	arg1	%					977:977	>10 %	973:977	>10 %	973:977	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	8	35	theme	major	945:949	arg1	C19 					985:988	C19 	985:988	C19 	985:988	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	8	35	theme	major	945:949	arg1	acids					966:970	The major cellular fatty acids	941:970	The major cellular fatty acids (>10 %)	941:978	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	4	36	theme	rRNA	564:567	arg1	similarity					583:592	a 16S rRNA gene sequence similarity	558:592	a 16S rRNA gene sequence similarity of 96.4 %	558:602	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	4	37	theme	sequence	449:456	arg1	identity					458:465	100 % sequence identity	443:465	100 % sequence identity	443:465	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	9	38	theme	G+C	1047:1049	arg1	%					1071:1071	35.1 mol%	1063:1071	35.1 mol%	1063:1071	The DNA G+C content was 35.1 mol%.
27453473	9	38	theme	G+C	1047:1049	arg1	content					1051:1057	The DNA G+C content	1039:1057	The DNA G+C content	1039:1057	The DNA G+C content was 35.1 mol%.
27453473	1	39	attach	isolated	72:79	arg2	bacterium					62:70	a thermophilic cellulose-degrading bacterium	27:70	a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor	27:114	nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor.
27453473	1	39	attach	isolated	72:79	arg1	reactor					108:114	a thermophilic biogas reactor	86:114	a thermophilic biogas reactor	86:114	nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor.
27453473	2	40	theme	Gram-stain-positive	173:191	arg1	bacteria					232:239	novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria	167:239	novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant	167:281	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	10	41	theme	genetic	1085:1091	arg1	differences					1108:1118	the genetic and phenotypic differences	1081:1118	the genetic and phenotypic differences to the most closely affiliated species	1081:1157	Due to the genetic and phenotypic differences to the most closely affiliated species, the isolates represent a novel species of the genus Herbinix within the family Lachnospiraceae, for which the name Herbinix luporum sp.
27453473	3	42	theme	novel	288:292	arg1	organisms					294:302	The novel organisms	284:302	The novel organisms	284:302	The novel organisms were able to degrade crystalline cellulose.
27453473	3	42	theme	novel	288:292	arg1	able					309:312	able	309:312	able	309:312	The novel organisms were able to degrade crystalline cellulose.
27453473	2	43	theme	novel	167:171	arg1	bacteria					232:239	novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria	167:239	novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant	167:281	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	10	44	theme	phenotypic	1097:1106	arg1	differences					1108:1118	the genetic and phenotypic differences	1081:1118	the genetic and phenotypic differences to the most closely affiliated species	1081:1157	Due to the genetic and phenotypic differences to the most closely affiliated species, the isolates represent a novel species of the genus Herbinix within the family Lachnospiraceae, for which the name Herbinix luporum sp.
27453473	4	45	theme	sequence	362:369	arg1	comparison					371:380	16S rRNA gene sequence comparison	348:380	16S rRNA gene sequence comparison	348:380	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	4	46	dep	Herbinix	500:507	arg1	hemicellulosilytica					509:527	hemicellulosilytica	509:527	hemicellulosilytica	509:527	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	6	47	theme	fermentative	836:847	arg1	utilization					860:870	fermentative cellobiose utilization	836:870	fermentative cellobiose utilization	836:870	Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization.
27453473	4	48	theme	gene	357:360	arg1	comparison					371:380	16S rRNA gene sequence comparison	348:380	16S rRNA gene sequence comparison	348:380	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	3	49	theme	crystalline	325:335	arg1	cellulose					337:345	crystalline cellulose	325:345	crystalline cellulose	325:345	The novel organisms were able to degrade crystalline cellulose.
27453473	2	50	theme	rod-shaped	221:230	arg1	bacteria					232:239	novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria	167:239	novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant	167:281	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	2	51	theme	bacteria	232:239	arg1	bacteria					232:239	novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria	167:239	novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant	167:281	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	2	51	theme	bacteria	232:239	arg1	group					158:162	a group	156:162	a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant	156:281	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	10	52	theme	Herbinix	1275:1282	arg1	sp					1292:1293	the name Herbinix luporum sp	1266:1293	the name Herbinix luporum sp	1266:1293	Due to the genetic and phenotypic differences to the most closely affiliated species, the isolates represent a novel species of the genus Herbinix within the family Lachnospiraceae, for which the name Herbinix luporum sp.
27453473	6	53	theme	cellobiose	849:858	arg1	utilization					860:870	fermentative cellobiose utilization	836:870	fermentative cellobiose utilization	836:870	Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization.
27453473	1	54	theme	thermophilic	29:40	arg1	bacterium					62:70	a thermophilic cellulose-degrading bacterium	27:70	a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor	27:114	nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor.
27453473	4	55	dep	isolates	401:408	arg1	isolates					401:408	the isolates	397:408	the isolates SD1DT, SD1G, SD1I and RK1P	397:435	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	4	55	dep	isolates	401:408	arg1	SD1G					417:420	SD1G	417:420	SD1G	417:420	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	4	55	dep	isolates	401:408	arg1	RK1P					432:435	RK1P	432:435	RK1P	432:435	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	4	55	dep	isolates	401:408	arg1	SD1I					423:426	SD1I	423:426	SD1I	423:426	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	4	56	theme	%	602:602	arg1	similarity					583:592	a 16S rRNA gene sequence similarity	558:592	a 16S rRNA gene sequence similarity of 96.4 %	558:602	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	4	57	theme	100 	443:446	arg1	%					447:447	%	447:447	%	447:447	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	8	58	theme	cellular	951:958	arg1	%					977:977	>10 %	973:977	>10 %	973:977	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	8	58	theme	cellular	951:958	arg1	C19 					985:988	C19 	985:988	C19 	985:988	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	8	58	theme	cellular	951:958	arg1	acids					966:970	The major cellular fatty acids	941:970	The major cellular fatty acids (>10 %)	941:978	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	1	59	dep	bacterium	62:70	arg1	nov.					21:24	nov.	21:24	nov.	21:24	nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor.
27453473	10	60	theme	luporum	1284:1290	arg1	sp					1292:1293	the name Herbinix luporum sp	1266:1293	the name Herbinix luporum sp	1266:1293	Due to the genetic and phenotypic differences to the most closely affiliated species, the isolates represent a novel species of the genus Herbinix within the family Lachnospiraceae, for which the name Herbinix luporum sp.
27453473	2	61	theme	biogas	270:275	arg1	plant					277:281	a thermophilic biogas plant	255:281	a thermophilic biogas plant	255:281	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	6	62	theme	major	810:814	arg1	end-products					816:827	major end-products	810:827	major end-products during fermentative cellobiose utilization	810:870	Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization.
27453473	8	63	theme	9,10	996:999	arg1	acetal					1010:1015	 0cyc 9,10 dimethyl acetal	990:1015	C19 : 0cyc 9,10 dimethyl acetal	985:1015	The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0.
27453473	2	64	theme	thermophilic	257:268	arg1	plant					277:281	a thermophilic biogas plant	255:281	a thermophilic biogas plant	255:281	Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant.
27453473	4	65	theme	rRNA	352:355	arg1	comparison					371:380	16S rRNA gene sequence comparison	348:380	16S rRNA gene sequence comparison	348:380	16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %.
27453473	10	66	theme	novel	1185:1189	arg1	species					1191:1197	a novel species	1183:1197	a novel species	1183:1197	Due to the genetic and phenotypic differences to the most closely affiliated species, the isolates represent a novel species of the genus Herbinix within the family Lachnospiraceae, for which the name Herbinix luporum sp.
26276149	2	0	from	influence	372:380	arg1	quality					391:397	flour quality	385:397	flour quality	385:397	The starch composition of wheat grain has a primary influence on flour quality.
26276149	8	1	contain	had	1147:1149	arg1	four					1040:1043	four	1040:1043	four	1040:1043	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	8	1	contain	had	1147:1149	arg1	one					1127:1129	one	1127:1129	one	1127:1129	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	8	1	contain	had	1147:1149	arg2	deletion					1153:1160	a deletion	1151:1160	a deletion of four nucleotides in the second exon	1151:1199	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	3	2	theme	glucose	438:444	arg1	polymers					446:453	glucose polymers	438:453	glucose polymers	438:453	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	0	3	from	subspecies	52:61	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat	0:80	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	0	3	from	subspecies	52:61	arg1	identification					86:99	identification	86:99	identification of two novel Wx-B1 alleles	86:126	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	0	4	theme	wheat	76:80	arg1	subspecies					52:61	three subspecies	46:61	three subspecies of hexaploid wheat	46:80	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	0	5	from	characterization	10:25	arg1	subspecies					52:61	three subspecies	46:61	three subspecies of hexaploid wheat	46:80	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	2	6	theme	flour	385:389	arg1	quality					391:397	flour quality	385:397	flour quality	385:397	The starch composition of wheat grain has a primary influence on flour quality.
26276149	1	7	theme	B1	144:145	arg1	alleles					152:158	Two novel Wx - B1 null alleles	129:158	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene	129:215	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene were characterized, whose effects on wheat quality could be different to those of the Wx - B1b allele.
26276149	3	8	theme	Wheat	400:404	arg1	starch					406:411	Wheat starch	400:411	Wheat starch	400:411	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	1	9	from	effects	243:249	arg1	quality					260:266	wheat quality	254:266	wheat quality	254:266	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene were characterized, whose effects on wheat quality could be different to those of the Wx - B1b allele.
26276149	9	10	theme	genetic	1327:1333	arg1	variability					1335:1345	the genetic variability	1323:1345	the genetic variability for this gene	1323:1359	These mutations were novel and provisionally catalogued as Wx-B1k and Wx-B1m, respectively, and could be used to enlarge the genetic variability for this gene.
26276149	3	11	theme	total	479:483	arg1	%					470:470	22-35%	465:470	22-35%	465:470	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	3	11	theme	total	479:483	arg1	total					479:483	total	479:483	total	479:483	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	5	12	theme	alleles	624:630	arg1	catalogue					606:614	the catalogue	602:614	the catalogue of waxy alleles available for breeders	602:653	Several studies have contributed to the catalogue of waxy alleles available for breeders, and the search for novel alleles of these and other proteins related to flour quality continues.
26276149	1	13	theme	B1b	308:310	arg1	allele					312:317	the Wx - B1b allele	299:317	the Wx - B1b allele	299:317	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene were characterized, whose effects on wheat quality could be different to those of the Wx - B1b allele.
26276149	6	14	theme	novel	809:813	arg1	Wx-B1k					830:835	Wx-B1k	830:835	Wx-B1k	830:835	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	6	14	theme	novel	809:813	arg1	alleles					821:827	two novel Wx-B1 alleles	805:827	two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat	805:901	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	6	14	theme	novel	809:813	arg1	Wx-B1m					841:846	Wx-B1m	841:846	Wx-B1m	841:846	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	8	15	theme	club	1136:1139	arg1	wheat					1141:1145	club wheat	1136:1145	club wheat	1136:1145	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	8	16	from	exon	1196:1199	arg1	deletion					1153:1160	a deletion	1151:1160	a deletion of four nucleotides in the second exon	1151:1199	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	0	17	theme	novel	108:112	arg1	alleles					120:126	two novel Wx-B1 alleles	104:126	two novel Wx-B1 alleles	104:126	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	8	18	theme	second	1189:1194	arg1	exon					1196:1199	the second exon	1185:1199	the second exon	1185:1199	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	6	19	theme	club	892:895	arg1	wheat					897:901	club wheat	892:901	club wheat	892:901	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	3	20	dep	types	429:433	arg1	types					429:433	two types	425:433	two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total)	425:522	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	3	20	dep	types	429:433	arg1	amylopectin					490:500	amylopectin	490:500	amylopectin (68-75% of the total)	490:522	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	3	20	dep	types	429:433	arg1	amylose					456:462	amylose	456:462	amylose (22-35% of the total)	456:484	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	8	21	theme	other	1022:1026	arg1	accessions					1028:1037	the other accessions	1018:1037	the other accessions	1018:1037	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	1	22	theme	null	147:150	arg1	alleles					152:158	Two novel Wx - B1 null alleles	129:158	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene	129:215	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene were characterized, whose effects on wheat quality could be different to those of the Wx - B1b allele.
26276149	6	23	theme	dwarf	882:886	arg1	collection					854:863	a collection	852:863	a collection of macha, Indian dwarf and club wheat	852:901	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	0	24	theme	Molecular	0:8	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat	0:80	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	3	25	dep	amylose	456:462	arg1	%					470:470	22-35%	465:470	22-35%	465:470	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	3	25	dep	amylose	456:462	arg1	total					479:483	total	479:483	total	479:483	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	0	26	from	alleles	35:41	arg1	subspecies					52:61	three subspecies	46:61	three subspecies of hexaploid wheat	46:80	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	6	27	from	collection	854:863	arg1	characterization					785:800	the characterization	781:800	the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat	781:901	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	6	28	theme	Indian	875:880	arg1	dwarf					882:886	Indian dwarf	875:886	Indian dwarf	875:886	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	1	29	theme	Wx	303:304	arg1	allele					312:317	the Wx - B1b allele	299:317	the Wx - B1b allele	299:317	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene were characterized, whose effects on wheat quality could be different to those of the Wx - B1b allele.
26276149	4	30	theme	waxy	551:554	arg1	proteins					556:563	waxy proteins	551:563	waxy proteins	551:563	Amylose is synthesized by waxy proteins.
26276149	5	31	theme	these	692:696	arg1	alleles					681:687	novel alleles	675:687	novel alleles of these and other proteins related to flour quality	675:740	Several studies have contributed to the catalogue of waxy alleles available for breeders, and the search for novel alleles of these and other proteins related to flour quality continues.
26276149	2	32	theme	starch	324:329	arg1	composition					331:341	The starch composition	320:341	The starch composition of wheat grain	320:356	The starch composition of wheat grain has a primary influence on flour quality.
26276149	0	33	theme	waxy	30:33	arg1	alleles					35:41	waxy alleles	30:41	waxy alleles in three subspecies of hexaploid wheat	30:80	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	1	34	theme	wheat	254:258	arg1	quality					260:266	wheat quality	254:266	wheat quality	254:266	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene were characterized, whose effects on wheat quality could be different to those of the Wx - B1b allele.
26276149	0	35	theme	alleles	120:126	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat	0:80	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	0	35	theme	alleles	120:126	arg1	identification					86:99	identification	86:99	identification of two novel Wx-B1 alleles	86:126	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	3	36	dep	amylopectin	490:500	arg1	%					508:508	68-75%	503:508	68-75% of the total	503:521	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	3	36	dep	amylopectin	490:500	arg1	total					517:521	total	517:521	total	517:521	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	8	37	theme	nucleotides	1170:1180	arg1	deletion					1153:1160	a deletion	1151:1160	a deletion of four nucleotides in the second exon	1151:1199	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	2	38	theme	primary	364:370	arg1	influence					372:380	a primary influence	362:380	a primary influence on flour quality	362:397	The starch composition of wheat grain has a primary influence on flour quality.
26276149	5	39	theme	other	702:706	arg1	proteins					708:715	other proteins	702:715	other proteins	702:715	Several studies have contributed to the catalogue of waxy alleles available for breeders, and the search for novel alleles of these and other proteins related to flour quality continues.
26276149	0	40	theme	Wx-B1	114:118	arg1	alleles					120:126	two novel Wx-B1 alleles	104:126	two novel Wx-B1 alleles	104:126	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	3	41	theme	polymers	446:453	arg1	types					429:433	two types	425:433	two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total)	425:522	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	3	41	theme	polymers	446:453	arg1	amylopectin					490:500	amylopectin	490:500	amylopectin (68-75% of the total)	490:522	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	3	41	theme	polymers	446:453	arg1	amylose					456:462	amylose	456:462	amylose (22-35% of the total)	456:484	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	1	42	theme	genetic	177:183	arg1	variability					185:195	the genetic variability	173:195	the genetic variability for this wheat gene	173:215	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene were characterized, whose effects on wheat quality could be different to those of the Wx - B1b allele.
26276149	8	43	from	deletion	1153:1160	arg1	exon					1196:1199	the second exon	1185:1199	the second exon	1185:1199	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	8	44	theme	bp	1095:1096	arg1	insertion					1080:1088	the insertion	1076:1088	the insertion of 4 bp within the seventh exon	1076:1120	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	0	45	theme	alleles	35:41	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat	0:80	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	0	45	theme	alleles	35:41	arg1	identification					86:99	identification	86:99	identification of two novel Wx-B1 alleles	86:126	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	6	46	theme	alleles	821:827	arg1	characterization					785:800	the characterization	781:800	the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat	781:901	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	7	47	theme	common	988:993	arg1	allele					1007:1012	the common Wx-B1b null allele	984:1012	the common Wx-B1b null allele	984:1012	Several accessions lacking Wx-B1 protein were detected, and some were caused by the common Wx-B1b null allele.
26276149	6	48	dep	alleles	821:827	arg1	Wx-B1k					830:835	Wx-B1k	830:835	Wx-B1k	830:835	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	6	48	dep	alleles	821:827	arg1	alleles					821:827	two novel Wx-B1 alleles	805:827	two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat	805:901	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	6	48	dep	alleles	821:827	arg1	Wx-B1m					841:846	Wx-B1m	841:846	Wx-B1m	841:846	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	7	49	theme	Wx-B1b	995:1000	arg1	allele					1007:1012	the common Wx-B1b null allele	984:1012	the common Wx-B1b null allele	984:1012	Several accessions lacking Wx-B1 protein were detected, and some were caused by the common Wx-B1b null allele.
26276149	5	50	theme	flour	728:732	arg1	quality					734:740	flour quality	728:740	flour quality	728:740	Several studies have contributed to the catalogue of waxy alleles available for breeders, and the search for novel alleles of these and other proteins related to flour quality continues.
26276149	2	51	theme	grain	352:356	arg1	composition					331:341	The starch composition	320:341	The starch composition of wheat grain	320:356	The starch composition of wheat grain has a primary influence on flour quality.
26276149	5	52	theme	novel	675:679	arg1	alleles					681:687	novel alleles	675:687	novel alleles of these and other proteins related to flour quality	675:740	Several studies have contributed to the catalogue of waxy alleles available for breeders, and the search for novel alleles of these and other proteins related to flour quality continues.
26276149	5	53	theme	waxy	619:622	arg1	alleles					624:630	waxy alleles	619:630	waxy alleles available for breeders	619:653	Several studies have contributed to the catalogue of waxy alleles available for breeders, and the search for novel alleles of these and other proteins related to flour quality continues.
26276149	5	54	theme	Several	566:572	arg1	studies					574:580	Several studies	566:580	Several studies	566:580	Several studies have contributed to the catalogue of waxy alleles available for breeders, and the search for novel alleles of these and other proteins related to flour quality continues.
26276149	7	55	theme	Wx-B1	931:935	arg1	protein					937:943	Wx-B1 protein	931:943	Wx-B1 protein	931:943	Several accessions lacking Wx-B1 protein were detected, and some were caused by the common Wx-B1b null allele.
26276149	7	56	theme	Several	904:910	arg1	accessions					912:921	Several accessions	904:921	Several accessions lacking Wx-B1 protein	904:943	Several accessions lacking Wx-B1 protein were detected, and some were caused by the common Wx-B1b null allele.
26276149	2	57	theme	wheat	346:350	arg1	grain					352:356	wheat grain	346:356	wheat grain	346:356	The starch composition of wheat grain has a primary influence on flour quality.
26276149	6	58	from	characterization	785:800	arg1	collection					854:863	a collection	852:863	a collection of macha, Indian dwarf and club wheat	852:901	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	6	59	from	alleles	821:827	arg1	collection					854:863	a collection	852:863	a collection of macha, Indian dwarf and club wheat	852:901	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	2	60	contain	has	358:360	arg2	influence					372:380	a primary influence	362:380	a primary influence on flour quality	362:397	The starch composition of wheat grain has a primary influence on flour quality.
26276149	2	60	contain	has	358:360	arg1	composition					331:341	The starch composition	320:341	The starch composition of wheat grain	320:356	The starch composition of wheat grain has a primary influence on flour quality.
26276149	3	61	theme	total	517:521	arg1	%					508:508	68-75%	503:508	68-75% of the total	503:521	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	3	61	theme	total	517:521	arg1	total					517:521	total	517:521	total	517:521	Wheat starch consists of two types of glucose polymers: amylose (22-35% of the total) and amylopectin (68-75% of the total).
26276149	9	62	used	used	1307:1310	arg2	Wx-B1m					1272:1277	Wx-B1m	1272:1277	Wx-B1m	1272:1277	These mutations were novel and provisionally catalogued as Wx-B1k and Wx-B1m, respectively, and could be used to enlarge the genetic variability for this gene.
26276149	9	62	used	used	1307:1310	arg2	Wx-B1k					1261:1266	Wx-B1k	1261:1266	Wx-B1k	1261:1266	These mutations were novel and provisionally catalogued as Wx-B1k and Wx-B1m, respectively, and could be used to enlarge the genetic variability for this gene.
26276149	9	62	used	used	1307:1310	arg2	mutations					1208:1216	These mutations	1202:1216	These mutations	1202:1216	These mutations were novel and provisionally catalogued as Wx-B1k and Wx-B1m, respectively, and could be used to enlarge the genetic variability for this gene.
26276149	5	63	theme	available	632:640	arg1	alleles					624:630	waxy alleles	619:630	waxy alleles available for breeders	619:653	Several studies have contributed to the catalogue of waxy alleles available for breeders, and the search for novel alleles of these and other proteins related to flour quality continues.
26276149	6	64	theme	wheat	897:901	arg1	collection					854:863	a collection	852:863	a collection of macha, Indian dwarf and club wheat	852:901	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	8	65	theme	seventh	1109:1115	arg1	exon					1117:1120	the seventh exon	1105:1120	the seventh exon	1105:1120	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	1	66	theme	wheat	206:210	arg1	gene					212:215	this wheat gene	201:215	this wheat gene	201:215	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene were characterized, whose effects on wheat quality could be different to those of the Wx - B1b allele.
26276149	0	67	theme	hexaploid	66:74	arg1	wheat					76:80	hexaploid wheat	66:80	hexaploid wheat	66:80	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	0	68	from	identification	86:99	arg1	subspecies					52:61	three subspecies	46:61	three subspecies of hexaploid wheat	46:80	Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles.
26276149	5	69	theme	proteins	708:715	arg1	alleles					681:687	novel alleles	675:687	novel alleles of these and other proteins related to flour quality	675:740	Several studies have contributed to the catalogue of waxy alleles available for breeders, and the search for novel alleles of these and other proteins related to flour quality continues.
26276149	1	70	theme	novel	133:137	arg1	alleles					152:158	Two novel Wx - B1 null alleles	129:158	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene	129:215	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene were characterized, whose effects on wheat quality could be different to those of the Wx - B1b allele.
26276149	8	71	theme	dwarf	1057:1061	arg1	wheat					1063:1067	Indian dwarf wheat	1050:1067	Indian dwarf wheat showed the insertion of 4 bp within the seventh exon	1050:1120	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	6	72	theme	macha	868:872	arg1	collection					854:863	a collection	852:863	a collection of macha, Indian dwarf and club wheat	852:901	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	8	73	theme	Indian	1050:1055	arg1	wheat					1063:1067	Indian dwarf wheat	1050:1067	Indian dwarf wheat showed the insertion of 4 bp within the seventh exon	1050:1120	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	7	74	theme	null	1002:1005	arg1	allele					1007:1012	the common Wx-B1b null allele	984:1012	the common Wx-B1b null allele	984:1012	Several accessions lacking Wx-B1 protein were detected, and some were caused by the common Wx-B1b null allele.
26276149	1	75	theme	Wx	139:140	arg1	alleles					152:158	Two novel Wx - B1 null alleles	129:158	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene	129:215	Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene were characterized, whose effects on wheat quality could be different to those of the Wx - B1b allele.
26276149	8	76	from	nucleotides	1170:1180	arg1	exon					1196:1199	the second exon	1185:1199	the second exon	1185:1199	Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon.
26276149	6	77	theme	Wx-B1	815:819	arg1	Wx-B1k					830:835	Wx-B1k	830:835	Wx-B1k	830:835	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	6	77	theme	Wx-B1	815:819	arg1	alleles					821:827	two novel Wx-B1 alleles	805:827	two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat	805:901	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26276149	6	77	theme	Wx-B1	815:819	arg1	Wx-B1m					841:846	Wx-B1m	841:846	Wx-B1m	841:846	In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat.
26179337	3	0	theme	ultra-low	688:696	arg1	content					708:714	ultra-low stachyose content	688:714	ultra-low stachyose content (0.5%)	688:721	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	3	0	theme	ultra-low	688:696	arg1	%					720:720	0.5%	717:720	0.5%	717:720	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	3	1	theme	content	565:571	arg1	sources					529:535	new sources	525:535	new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection	525:624	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	3	2	theme	modified	540:547	arg1	content					565:571	modified oligosaccharide content	540:571	modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection	540:624	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	1	3	theme	gene	186:189	arg1	mutant					149:154	a mutant	147:154	a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future	147:301	We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future.
26179337	4	4	theme	PI	831:832	arg1	603176A					834:840	PI 603176A	831:840	PI 603176A	831:840	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	6	5	theme	STS	1124:1126	arg1	responsible					1136:1146	responsible	1136:1146	responsible	1136:1146	These observations provided strong evidence that the STS gene is responsible for stachyose biosynthesis in the soybean plant.
26179337	6	5	theme	STS	1124:1126	arg1	gene					1128:1131	the STS gene	1120:1131	the STS gene	1120:1131	These observations provided strong evidence that the STS gene is responsible for stachyose biosynthesis in the soybean plant.
26179337	1	6	theme	reduced	203:209	arg1	content					221:227	reduced stachyose content	203:227	reduced stachyose content which benefit the soybean seed composition breeding program in the future	203:301	We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future.
26179337	7	7	theme	defective	1308:1316	arg1	function					1326:1333	defective protein function	1308:1333	defective protein function	1308:1333	Expression of the sts gene remained at the normal level, suggesting the loss of function in the gene is due to defective protein function.
26179337	5	8	theme	low	1027:1029	arg1	content					1041:1047	low stachyose content	1027:1047	low stachyose content in the progeny lines	1027:1068	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	8	9	theme	stachyose	1383:1391	arg1	content					1393:1399	low stachyose content	1379:1399	low stachyose content	1379:1399	This gene-based perfect genetic marker for low stachyose content can be useful for marker-assisted selection in soybean molecular breeding programs.
26179337	7	10	theme	protein	1318:1324	arg1	function					1326:1333	defective protein function	1308:1333	defective protein function	1308:1333	Expression of the sts gene remained at the normal level, suggesting the loss of function in the gene is due to defective protein function.
26179337	1	11	from	program	281:287	arg1	future					296:301	future	296:301	future	296:301	We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future.
26179337	2	12	contain	have	409:412	arg1	oligosaccharides					386:401	increased sucrose and reduced raffinose family oligosaccharides	339:401	increased sucrose and reduced raffinose family oligosaccharides	339:401	It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency.
26179337	2	12	contain	have	409:412	arg2	impact					425:430	a positive impact	414:430	a positive impact on the world's feed industry	414:459	It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency.
26179337	3	13	theme	Germplasm	605:613	arg1	Collection					615:624	the USDA Soybean Germplasm Collection	588:624	the USDA Soybean Germplasm Collection	588:624	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	3	14	theme	USDA	592:595	arg1	Collection					615:624	the USDA Soybean Germplasm Collection	588:624	the USDA Soybean Germplasm Collection	588:624	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	5	15	theme	33-bp	907:911	arg1	area					922:925	this 33-bp deletion area	902:925	this 33-bp deletion area	902:925	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	8	16	theme	marker-assisted	1419:1433	arg1	selection					1435:1443	marker-assisted selection	1419:1443	marker-assisted selection in soybean molecular breeding programs	1419:1482	This gene-based perfect genetic marker for low stachyose content can be useful for marker-assisted selection in soybean molecular breeding programs.
26179337	0	17	theme	stachyose	85:93	arg1	content					95:101	reduced stachyose content	77:101	reduced stachyose content in soybean	77:112	Identification and characterization of a stachyose synthase gene controlling reduced stachyose content in soybean.
26179337	1	18	theme	stachyose	167:175	arg1	gene					186:189	soybean stachyose synthase gene	159:189	soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future	159:301	We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future.
26179337	8	19	theme	molecular	1456:1464	arg1	programs					1475:1482	soybean molecular breeding programs	1448:1482	soybean molecular breeding programs	1448:1482	This gene-based perfect genetic marker for low stachyose content can be useful for marker-assisted selection in soybean molecular breeding programs.
26179337	3	20	from	content	565:571	arg1	subset					578:583	a subset	576:583	a subset of the USDA Soybean Germplasm Collection	576:624	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	4	21	from	mutant	755:760	arg1	gene					797:800	the putative stachyose synthase gene	765:800	the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A	765:840	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	4	22	theme	33-bp	740:744	arg1	mutant					755:760	a 33-bp deletion mutant	738:760	a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A	738:840	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	3	23	theme	PI	666:667	arg1	603176A					670:676	plant introduction (PI) 603176A	646:676	plant introduction (PI) 603176A	646:676	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	4	24	theme	STS	803:805	arg1	Glyma19g40550					813:825	Glyma19g40550	813:825	Glyma19g40550	813:825	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	4	24	theme	STS	803:805	arg1	gene					807:810	STS gene	803:810	STS gene	803:810	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	4	25	dep	gene	797:800	arg1	Glyma19g40550					813:825	Glyma19g40550	813:825	Glyma19g40550	813:825	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	4	25	dep	gene	797:800	arg1	gene					807:810	STS gene	803:810	STS gene	803:810	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	5	26	theme	F	987:987	arg1	population					993:1002	a F 4:5 population	985:1002	a F 4:5 population	985:1002	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	3	27	theme	introduction	652:663	arg1	603176A					670:676	plant introduction (PI) 603176A	646:676	plant introduction (PI) 603176A	646:676	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	8	28	theme	perfect	1352:1358	arg1	marker					1368:1373	This gene-based perfect genetic marker	1336:1373	This gene-based perfect genetic marker for low stachyose content	1336:1399	This gene-based perfect genetic marker for low stachyose content can be useful for marker-assisted selection in soybean molecular breeding programs.
26179337	2	29	theme	sucrose	349:355	arg1	oligosaccharides					386:401	increased sucrose and reduced raffinose family oligosaccharides	339:401	increased sucrose and reduced raffinose family oligosaccharides	339:401	It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency.
26179337	0	30	theme	synthase	51:58	arg1	gene					60:63	a stachyose synthase gene	39:63	a stachyose synthase gene	39:63	Identification and characterization of a stachyose synthase gene controlling reduced stachyose content in soybean.
26179337	7	31	theme	normal	1240:1245	arg1	level					1247:1251	the normal level	1236:1251	the normal level	1236:1251	Expression of the sts gene remained at the normal level, suggesting the loss of function in the gene is due to defective protein function.
26179337	4	32	theme	stachyose	778:786	arg1	gene					797:800	the putative stachyose synthase gene	765:800	the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A	765:840	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	5	33	theme	2:3	965:967	arg1	populations					969:979	two F 2:3 populations	959:979	two F 2:3 populations	959:979	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	7	34	from	loss	1269:1272	arg1	gene					1293:1296	the gene	1289:1296	the gene	1289:1296	Expression of the sts gene remained at the normal level, suggesting the loss of function in the gene is due to defective protein function.
26179337	8	35	theme	low	1379:1381	arg1	content					1393:1399	low stachyose content	1379:1399	low stachyose content	1379:1399	This gene-based perfect genetic marker for low stachyose content can be useful for marker-assisted selection in soybean molecular breeding programs.
26179337	3	36	theme	new	525:527	arg1	sources					529:535	new sources	525:535	new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection	525:624	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	1	37	theme	seed	255:258	arg1	program					281:287	the soybean seed composition breeding program	243:287	the soybean seed composition breeding program in the future	243:301	We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future.
26179337	7	38	theme	gene	1219:1222	arg1	Expression					1197:1206	Expression	1197:1206	Expression of the sts gene	1197:1222	Expression of the sts gene remained at the normal level, suggesting the loss of function in the gene is due to defective protein function.
26179337	2	39	theme	reduced	361:367	arg1	raffinose					369:377	reduced raffinose	361:377	reduced raffinose	361:377	It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency.
26179337	1	40	theme	breeding	272:279	arg1	program					281:287	the soybean seed composition breeding program	243:287	the soybean seed composition breeding program in the future	243:301	We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future.
26179337	4	41	theme	603176A	834:840	arg1	gene					797:800	the putative stachyose synthase gene	765:800	the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A	765:840	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	3	42	theme	stachyose	698:706	arg1	content					708:714	ultra-low stachyose content	688:714	ultra-low stachyose content (0.5%)	688:721	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	3	42	theme	stachyose	698:706	arg1	%					720:720	0.5%	717:720	0.5%	717:720	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	3	43	theme	oligosaccharide	549:563	arg1	content					565:571	modified oligosaccharide content	540:571	modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection	540:624	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	3	44	from	sources	529:535	arg1	subset					578:583	a subset	576:583	a subset of the USDA Soybean Germplasm Collection	576:624	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	1	45	theme	synthase	177:184	arg1	gene					186:189	soybean stachyose synthase gene	159:189	soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future	159:301	We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future.
26179337	1	46	theme	stachyose	211:219	arg1	content					221:227	reduced stachyose content	203:227	reduced stachyose content which benefit the soybean seed composition breeding program in the future	203:301	We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future.
26179337	0	47	from	content	95:101	arg1	soybean					106:112	soybean	106:112	soybean	106:112	Identification and characterization of a stachyose synthase gene controlling reduced stachyose content in soybean.
26179337	5	48	theme	stachyose	1031:1039	arg1	content					1041:1047	low stachyose content	1027:1047	low stachyose content in the progeny lines	1027:1068	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	3	49	theme	Soybean	597:603	arg1	Collection					615:624	the USDA Soybean Germplasm Collection	588:624	the USDA Soybean Germplasm Collection	588:624	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	5	50	theme	co-dominate	845:855	arg1	marker					863:868	A co-dominate indel marker	843:868	A co-dominate indel marker	843:868	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	8	51	from	selection	1435:1443	arg1	programs					1475:1482	soybean molecular breeding programs	1448:1482	soybean molecular breeding programs	1448:1482	This gene-based perfect genetic marker for low stachyose content can be useful for marker-assisted selection in soybean molecular breeding programs.
26179337	6	52	theme	soybean	1182:1188	arg1	plant					1190:1194	the soybean plant	1178:1194	the soybean plant	1178:1194	These observations provided strong evidence that the STS gene is responsible for stachyose biosynthesis in the soybean plant.
26179337	3	53	from	subset	578:583	arg1	sources					529:535	new sources	525:535	new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection	525:624	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	0	54	theme	reduced	77:83	arg1	content					95:101	reduced stachyose content	77:101	reduced stachyose content in soybean	77:112	Identification and characterization of a stachyose synthase gene controlling reduced stachyose content in soybean.
26179337	3	55	theme	Collection	615:624	arg1	subset					578:583	a subset	576:583	a subset of the USDA Soybean Germplasm Collection	576:624	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	5	56	theme	indel	857:861	arg1	marker					863:868	A co-dominate indel marker	843:868	A co-dominate indel marker	843:868	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	8	57	theme	soybean	1448:1454	arg1	programs					1475:1482	soybean molecular breeding programs	1448:1482	soybean molecular breeding programs	1448:1482	This gene-based perfect genetic marker for low stachyose content can be useful for marker-assisted selection in soybean molecular breeding programs.
26179337	6	58	theme	stachyose	1152:1160	arg1	biosynthesis					1162:1173	stachyose biosynthesis	1152:1173	stachyose biosynthesis in the soybean plant	1152:1194	These observations provided strong evidence that the STS gene is responsible for stachyose biosynthesis in the soybean plant.
26179337	6	59	from	biosynthesis	1162:1173	arg1	plant					1190:1194	the soybean plant	1178:1194	the soybean plant	1178:1194	These observations provided strong evidence that the STS gene is responsible for stachyose biosynthesis in the soybean plant.
26179337	1	60	theme	soybean	159:165	arg1	gene					186:189	soybean stachyose synthase gene	159:189	soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future	159:301	We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future.
26179337	2	61	theme	feed	492:495	arg1	efficiency					497:506	feed efficiency	492:506	feed efficiency	492:506	It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency.
26179337	8	62	theme	breeding	1466:1473	arg1	programs					1475:1482	soybean molecular breeding programs	1448:1482	soybean molecular breeding programs	1448:1482	This gene-based perfect genetic marker for low stachyose content can be useful for marker-assisted selection in soybean molecular breeding programs.
26179337	5	63	from	content	1041:1047	arg1	lines					1064:1068	the progeny lines	1052:1068	the progeny lines	1052:1068	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	4	64	theme	putative	769:776	arg1	gene					797:800	the putative stachyose synthase gene	765:800	the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A	765:840	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	2	65	from	impact	425:430	arg1	industry					452:459	the world's feed industry	435:459	the world's feed industry	435:459	It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency.
26179337	4	66	theme	deletion	746:753	arg1	mutant					755:760	a 33-bp deletion mutant	738:760	a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A	738:840	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	5	67	theme	progeny	1056:1062	arg1	lines					1064:1068	the progeny lines	1052:1068	the progeny lines	1052:1068	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	7	68	theme	function	1277:1284	arg1	due					1301:1303	due	1301:1303	due	1301:1303	Expression of the sts gene remained at the normal level, suggesting the loss of function in the gene is due to defective protein function.
26179337	7	68	theme	function	1277:1284	arg1	loss					1269:1272	the loss	1265:1272	the loss of function in the gene	1265:1296	Expression of the sts gene remained at the normal level, suggesting the loss of function in the gene is due to defective protein function.
26179337	5	69	theme	deletion	913:920	arg1	area					922:925	this 33-bp deletion area	902:925	this 33-bp deletion area	902:925	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	2	70	theme	feed	447:450	arg1	industry					452:459	the world's feed industry	435:459	the world's feed industry	435:459	It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency.
26179337	2	71	theme	family	379:384	arg1	oligosaccharides					386:401	increased sucrose and reduced raffinose family oligosaccharides	339:401	increased sucrose and reduced raffinose family oligosaccharides	339:401	It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency.
26179337	0	72	theme	stachyose	41:49	arg1	gene					60:63	a stachyose synthase gene	39:63	a stachyose synthase gene	39:63	Identification and characterization of a stachyose synthase gene controlling reduced stachyose content in soybean.
26179337	3	73	theme	plant	646:650	arg1	603176A					670:676	plant introduction (PI) 603176A	646:676	plant introduction (PI) 603176A	646:676	We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%).
26179337	2	74	theme	positive	416:423	arg1	impact					425:430	a positive impact	414:430	a positive impact on the world's feed industry	414:459	It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency.
26179337	0	75	theme	gene	60:63	arg1	characterization					19:34	characterization	19:34	characterization	19:34	Identification and characterization of a stachyose synthase gene controlling reduced stachyose content in soybean.
26179337	0	75	theme	gene	60:63	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and characterization of a stachyose synthase gene controlling reduced stachyose content in soybean.
26179337	5	76	theme	4:5	989:991	arg1	population					993:1002	a F 4:5 population	985:1002	a F 4:5 population	985:1002	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	6	77	theme	strong	1099:1104	arg1	evidence					1106:1113	strong evidence that the STS gene is responsible for stachyose biosynthesis in the soybean plant	1099:1194	strong evidence that the STS gene is responsible for stachyose biosynthesis in the soybean plant	1099:1194	These observations provided strong evidence that the STS gene is responsible for stachyose biosynthesis in the soybean plant.
26179337	8	78	theme	gene-based	1341:1350	arg1	marker					1368:1373	This gene-based perfect genetic marker	1336:1373	This gene-based perfect genetic marker for low stachyose content	1336:1399	This gene-based perfect genetic marker for low stachyose content can be useful for marker-assisted selection in soybean molecular breeding programs.
26179337	2	79	theme	increased	339:347	arg1	sucrose					349:355	increased sucrose	339:355	increased sucrose	339:355	It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency.
26179337	5	80	theme	F	963:963	arg1	populations					969:979	two F 2:3 populations	959:979	two F 2:3 populations	959:979	A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines.
26179337	8	81	theme	genetic	1360:1366	arg1	marker					1368:1373	This gene-based perfect genetic marker	1336:1373	This gene-based perfect genetic marker for low stachyose content	1336:1399	This gene-based perfect genetic marker for low stachyose content can be useful for marker-assisted selection in soybean molecular breeding programs.
26179337	1	82	theme	soybean	247:253	arg1	program					281:287	the soybean seed composition breeding program	243:287	the soybean seed composition breeding program in the future	243:301	We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future.
26179337	7	83	theme	sts	1215:1217	arg1	gene					1219:1222	the sts gene	1211:1222	the sts gene	1211:1222	Expression of the sts gene remained at the normal level, suggesting the loss of function in the gene is due to defective protein function.
26179337	4	84	theme	synthase	788:795	arg1	gene					797:800	the putative stachyose synthase gene	765:800	the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A	765:840	We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A.
26179337	2	85	theme	raffinose	369:377	arg1	oligosaccharides					386:401	increased sucrose and reduced raffinose family oligosaccharides	339:401	increased sucrose and reduced raffinose family oligosaccharides	339:401	It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency.
26179337	1	86	theme	composition	260:270	arg1	program					281:287	the soybean seed composition breeding program	243:287	the soybean seed composition breeding program in the future	243:301	We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future.
24848698	4	0	theme	L-lysine	862:869	arg1	dynamics					823:830	the metabolic dynamics	809:830	the metabolic dynamics of butyrate, L-phenylalanine, L-lysine and tyramine	809:882	Furthermore, the metabolic dynamics of butyrate, L-phenylalanine, L-lysine and tyramine were positively correlated with that of these bacteria and IgA production, whereas p-cresol was negatively correlated.
24848698	1	1	theme	health-promoting	169:184	arg1	properties					186:195	its health-promoting properties	165:195	its health-promoting properties	165:195	Fructooligosaccharide (FOS), a prebiotic well known for its health-promoting properties, can improve the human gut ecosystem most likely through changes in its microbial composition.
24848698	5	2	theme	gut	1200:1202	arg1	ecosystem					1204:1212	the human gut ecosystem	1190:1212	the human gut ecosystem	1190:1212	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	1	3	theme	microbial	269:277	arg1	composition					279:289	its microbial composition	265:289	its microbial composition	265:289	Fructooligosaccharide (FOS), a prebiotic well known for its health-promoting properties, can improve the human gut ecosystem most likely through changes in its microbial composition.
24848698	4	4	theme	IgA	943:945	arg1	production					947:956	IgA production	943:956	IgA production	943:956	Furthermore, the metabolic dynamics of butyrate, L-phenylalanine, L-lysine and tyramine were positively correlated with that of these bacteria and IgA production, whereas p-cresol was negatively correlated.
24848698	4	5	theme	tyramine	875:882	arg1	dynamics					823:830	the metabolic dynamics	809:830	the metabolic dynamics of butyrate, L-phenylalanine, L-lysine and tyramine	809:882	Furthermore, the metabolic dynamics of butyrate, L-phenylalanine, L-lysine and tyramine were positively correlated with that of these bacteria and IgA production, whereas p-cresol was negatively correlated.
24848698	2	6	theme	detailed	305:312	arg1	s					324:324	the detailed mechanism(s)	301:325	the detailed mechanism(s) of action of FOS in the modulation of the gut ecosystem	301:381	However, the detailed mechanism(s) of action of FOS in the modulation of the gut ecosystem remain(s) obscure.
24848698	2	7	from	s	324:324	arg1	modulation					351:360	the modulation	347:360	the modulation of the gut ecosystem	347:381	However, the detailed mechanism(s) of action of FOS in the modulation of the gut ecosystem remain(s) obscure.
24848698	3	8	theme	mucosal	710:716	arg1	IgA					718:720	mucosal IgA	710:720	mucosal IgA	710:720	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	3	9	theme	large	527:531	arg1	differences					549:559	large interindividual differences	527:559	large interindividual differences	527:559	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	3	10	theme	significant	482:492	arg1	features					494:501	any significant features	478:501	any significant features due to the existence of large interindividual differences	478:559	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	3	11	theme	IgA	718:720	arg1	induction					697:705	the induction	693:705	the induction of mucosal IgA by FOS supplementation	693:743	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	4	12	theme	butyrate	835:842	arg1	dynamics					823:830	the metabolic dynamics	809:830	the metabolic dynamics of butyrate, L-phenylalanine, L-lysine and tyramine	809:882	Furthermore, the metabolic dynamics of butyrate, L-phenylalanine, L-lysine and tyramine were positively correlated with that of these bacteria and IgA production, whereas p-cresol was negatively correlated.
24848698	3	13	theme	metabolites	448:458	arg1	methods					414:420	Traditional methods	402:420	Traditional methods of profiling microbes and metabolites	402:458	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	5	14	with	analysis	1047:1054	arg1	approaches					1067:1076	omics approaches	1061:1076	omics approaches	1061:1076	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	2	15	theme	ecosystem	373:381	arg1	modulation					351:360	the modulation	347:360	the modulation of the gut ecosystem	347:381	However, the detailed mechanism(s) of action of FOS in the modulation of the gut ecosystem remain(s) obscure.
24848698	5	16	theme	gut	1120:1122	arg1	network					1134:1140	the gut molecular network	1116:1140	the gut molecular network	1116:1140	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	4	17	theme	L-phenylalanine	845:859	arg1	dynamics					823:830	the metabolic dynamics	809:830	the metabolic dynamics of butyrate, L-phenylalanine, L-lysine and tyramine	809:882	Furthermore, the metabolic dynamics of butyrate, L-phenylalanine, L-lysine and tyramine were positively correlated with that of these bacteria and IgA production, whereas p-cresol was negatively correlated.
24848698	2	18	theme	gut	369:371	arg1	ecosystem					373:381	the gut ecosystem	365:381	the gut ecosystem	365:381	However, the detailed mechanism(s) of action of FOS in the modulation of the gut ecosystem remain(s) obscure.
24848698	0	19	theme	Multiple	0:7	arg1	omics					9:13	Multiple omics	0:13	Multiple omics	0:13	Multiple omics uncovers host-gut microbial mutualism during prebiotic fructooligosaccharide supplementation.
24848698	3	20	theme	differences	549:559	arg1	existence					514:522	the existence	510:522	the existence of large interindividual differences	510:559	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	3	21	theme	Traditional	402:412	arg1	methods					414:420	Traditional methods	402:420	Traditional methods of profiling microbes and metabolites	402:458	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	0	22	theme	host-gut	24:31	arg1	mutualism					43:51	host-gut microbial mutualism	24:51	host-gut microbial mutualism	24:51	Multiple omics uncovers host-gut microbial mutualism during prebiotic fructooligosaccharide supplementation.
24848698	4	23	theme	metabolic	813:821	arg1	dynamics					823:830	the metabolic dynamics	809:830	the metabolic dynamics of butyrate, L-phenylalanine, L-lysine and tyramine	809:882	Furthermore, the metabolic dynamics of butyrate, L-phenylalanine, L-lysine and tyramine were positively correlated with that of these bacteria and IgA production, whereas p-cresol was negatively correlated.
24848698	3	24	theme	interindividual	533:547	arg1	differences					549:559	large interindividual differences	527:559	large interindividual differences	527:559	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	1	25	theme	human	214:218	arg1	ecosystem					224:232	the human gut ecosystem	210:232	the human gut ecosystem	210:232	Fructooligosaccharide (FOS), a prebiotic well known for its health-promoting properties, can improve the human gut ecosystem most likely through changes in its microbial composition.
24848698	3	26	theme	A	653:653	arg1	measurements					661:672	faecal immunoglobulin A (IgA) measurements	631:672	faecal immunoglobulin A (IgA) measurements	631:672	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	2	27	theme	mechanism	314:322	arg1	s					324:324	the detailed mechanism(s)	301:325	the detailed mechanism(s) of action of FOS in the modulation of the gut ecosystem	301:381	However, the detailed mechanism(s) of action of FOS in the modulation of the gut ecosystem remain(s) obscure.
24848698	1	28	theme	gut	220:222	arg1	ecosystem					224:232	the human gut ecosystem	210:232	the human gut ecosystem	210:232	Fructooligosaccharide (FOS), a prebiotic well known for its health-promoting properties, can improve the human gut ecosystem most likely through changes in its microbial composition.
24848698	5	29	theme	human	1194:1198	arg1	ecosystem					1204:1212	the human gut ecosystem	1190:1212	the human gut ecosystem	1190:1212	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	3	30	theme	FOS	725:727	arg1	supplementation					729:743	FOS supplementation	725:743	FOS supplementation	725:743	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	3	31	theme	microbe-metabolite	576:593	arg1	approach					607:614	our novel microbe-metabolite correlation approach	566:614	our novel microbe-metabolite correlation approach	566:614	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	3	32	theme	profiling	425:433	arg1	microbes					435:442	profiling microbes	425:442	profiling microbes	425:442	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	0	33	theme	microbial	33:41	arg1	mutualism					43:51	host-gut microbial mutualism	24:51	host-gut microbial mutualism	24:51	Multiple omics uncovers host-gut microbial mutualism during prebiotic fructooligosaccharide supplementation.
24848698	3	34	theme	faecal	631:636	arg1	A					653:653	faecal immunoglobulin A	631:653	faecal immunoglobulin A (IgA) measurements	631:672	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	3	34	theme	faecal	631:636	arg1	IgA					656:658	IgA	656:658	IgA	656:658	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	3	35	theme	correlation	595:605	arg1	approach					607:614	our novel microbe-metabolite correlation approach	566:614	our novel microbe-metabolite correlation approach	566:614	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	3	36	theme	microbes	435:442	arg1	methods					414:420	Traditional methods	402:420	Traditional methods of profiling microbes and metabolites	402:458	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	0	37	theme	prebiotic	60:68	arg1	supplementation					92:106	prebiotic fructooligosaccharide supplementation	60:106	prebiotic fructooligosaccharide supplementation	60:106	Multiple omics uncovers host-gut microbial mutualism during prebiotic fructooligosaccharide supplementation.
24848698	1	38	from	changes	254:260	arg1	composition					279:289	its microbial composition	265:289	its microbial composition	265:289	Fructooligosaccharide (FOS), a prebiotic well known for its health-promoting properties, can improve the human gut ecosystem most likely through changes in its microbial composition.
24848698	3	39	theme	immunoglobulin	638:651	arg1	A					653:653	faecal immunoglobulin A	631:653	faecal immunoglobulin A (IgA) measurements	631:672	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	3	39	theme	immunoglobulin	638:651	arg1	IgA					656:658	IgA	656:658	IgA	656:658	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	5	40	theme	focused	1023:1029	arg1	analysis					1047:1054	our focused intraindividual analysis	1019:1054	our focused intraindividual analysis with omics approaches	1019:1076	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	5	40	theme	focused	1023:1029	arg1	strategy					1092:1099	a powerful strategy	1081:1099	a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem	1081:1212	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	2	41	theme	FOS	340:342	arg1	action					330:335	action	330:335	action of FOS	330:342	However, the detailed mechanism(s) of action of FOS in the modulation of the gut ecosystem remain(s) obscure.
24848698	5	42	theme	new	1162:1164	arg1	vista					1166:1170	a new vista	1160:1170	a new vista for understanding the human gut ecosystem	1160:1212	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	5	43	theme	intraindividual	1031:1045	arg1	analysis					1047:1054	our focused intraindividual analysis	1019:1054	our focused intraindividual analysis with omics approaches	1019:1076	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	5	43	theme	intraindividual	1031:1045	arg1	strategy					1092:1099	a powerful strategy	1081:1099	a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem	1081:1212	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	3	44	theme	novel	570:574	arg1	approach					607:614	our novel microbe-metabolite correlation approach	566:614	our novel microbe-metabolite correlation approach	566:614	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	5	45	theme	powerful	1083:1090	arg1	analysis					1047:1054	our focused intraindividual analysis	1019:1054	our focused intraindividual analysis with omics approaches	1019:1076	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	5	45	theme	powerful	1083:1090	arg1	strategy					1092:1099	a powerful strategy	1081:1099	a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem	1081:1212	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	2	46	theme	action	330:335	arg1	s					324:324	the detailed mechanism(s)	301:325	the detailed mechanism(s) of action of FOS in the modulation of the gut ecosystem	301:381	However, the detailed mechanism(s) of action of FOS in the modulation of the gut ecosystem remain(s) obscure.
24848698	5	47	theme	omics	1061:1065	arg1	approaches					1067:1076	omics approaches	1061:1076	omics approaches	1061:1076	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	3	48	theme	due	503:505	arg1	features					494:501	any significant features	478:501	any significant features due to the existence of large interindividual differences	478:559	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	3	49	theme	specific	777:784	arg1	bacteria					786:793	specific bacteria	777:793	specific bacteria	777:793	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
24848698	0	50	theme	fructooligosaccharide	70:90	arg1	supplementation					92:106	prebiotic fructooligosaccharide supplementation	60:106	prebiotic fructooligosaccharide supplementation	60:106	Multiple omics uncovers host-gut microbial mutualism during prebiotic fructooligosaccharide supplementation.
24848698	5	51	theme	molecular	1124:1132	arg1	network					1134:1140	the gut molecular network	1116:1140	the gut molecular network	1116:1140	Taken together, our focused intraindividual analysis with omics approaches is a powerful strategy for uncovering the gut molecular network and could provide a new vista for understanding the human gut ecosystem.
24848698	3	52	theme	bacteria	786:793	arg1	presence					765:772	the presence	761:772	the presence of specific bacteria	761:793	Traditional methods of profiling microbes and metabolites could barely show any significant features due to the existence of large interindividual differences, but our novel microbe-metabolite correlation approach, combined with faecal immunoglobulin A (IgA) measurements, has revealed that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific bacteria.
25311962	11	0	theme	relative	1257:1264	arg1	rate					1273:1276	the relative growth rate	1253:1276	the relative growth rate of mouse embryo fibroblasts (MEFs) on CMs	1253:1318	In contrast, the relative growth rate of mouse embryo fibroblasts (MEFs) on CMs decreased gradually.
25311962	4	1	theme	good	656:659	arg1	flexibility					672:682	good mechanical flexibility	656:682	good mechanical flexibility	656:682	All microspheres had smooth surfaces and good mechanical flexibility, and all could pass through a 5F catheter.
25311962	12	2	theme	MEFs	1403:1406	arg1	growth					1393:1398	the growth	1389:1398	the growth of MEFs	1389:1406	The results suggested that ACMs could stimulate the growth of MEFs, and CMs were not cytotoxic to MEFs.
25311962	11	3	theme	growth	1266:1271	arg1	rate					1273:1276	the relative growth rate	1253:1276	the relative growth rate of mouse embryo fibroblasts (MEFs) on CMs	1253:1318	In contrast, the relative growth rate of mouse embryo fibroblasts (MEFs) on CMs decreased gradually.
25311962	10	4	contain	had	1172:1174	arg1	ACMs					1157:1160	ACMs	1157:1160	ACMs	1157:1160	Cytotoxicity tests indicated that ACMs initially had less cell attached proliferation but increased with incubation.
25311962	10	4	contain	had	1172:1174	arg2	proliferation					1195:1207	less cell attached proliferation	1176:1207	less cell attached proliferation	1176:1207	Cytotoxicity tests indicated that ACMs initially had less cell attached proliferation but increased with incubation.
25311962	7	5	theme	more	987:990	arg1	clots					998:1002	more blood clots	987:1002	more blood clots	987:1002	Furthermore, CMs caused more blood clots than ACMs.
25311962	3	6	theme	scanning	489:496	arg1	TEM					519:521	TEM	519:521	TEM	519:521	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	3	6	theme	scanning	489:496	arg1	microscopy					507:516	scanning electron microscopy	489:516	scanning electron microscopy (TEM)	489:522	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	11	7	theme	fibroblasts	1294:1304	arg1	rate					1273:1276	the relative growth rate	1253:1276	the relative growth rate of mouse embryo fibroblasts (MEFs) on CMs	1253:1318	In contrast, the relative growth rate of mouse embryo fibroblasts (MEFs) on CMs decreased gradually.
25311962	4	8	theme	5F	714:715	arg1	catheter					717:724	a 5F catheter	712:724	a 5F catheter	712:724	All microspheres had smooth surfaces and good mechanical flexibility, and all could pass through a 5F catheter.
25311962	3	9	theme	laser	533:537	arg1	DLS					557:559	DLS	557:559	DLS	557:559	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	3	9	theme	laser	533:537	arg1	scattering					545:554	dynamic laser light scattering	525:554	dynamic laser light scattering (DLS)	525:560	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	2	10	theme	W/O	274:276	arg1	method					308:313	water-in-oil (W/O) emulsification cross-linking method	260:313	water-in-oil (W/O) emulsification cross-linking method	260:313	Chitosan microspheres (CMs) were prepared via water-in-oil (W/O) emulsification cross-linking method, and acetylated chitosan microspheres (ACMs) were obtained by acetylation of CMs.
25311962	11	11	theme	mouse	1281:1285	arg1	MEFs					1307:1310	MEFs	1307:1310	MEFs	1307:1310	In contrast, the relative growth rate of mouse embryo fibroblasts (MEFs) on CMs decreased gradually.
25311962	11	11	theme	mouse	1281:1285	arg1	fibroblasts					1294:1304	mouse embryo fibroblasts	1281:1304	mouse embryo fibroblasts (MEFs)	1281:1311	In contrast, the relative growth rate of mouse embryo fibroblasts (MEFs) on CMs decreased gradually.
25311962	8	12	dep	CMs	1047:1049	arg1	%					1054:1054	<5%	1052:1054	<5% of the time	1052:1066	ACMs caused hemolysis less than CMs (<5% of the time).
25311962	8	12	dep	CMs	1047:1049	arg1	time					1063:1066	the time	1059:1066	the time	1059:1066	ACMs caused hemolysis less than CMs (<5% of the time).
25311962	3	13	dep	Fourier	567:573	arg1	transform					575:583	transform	575:583	transform infrared spectrometer (FTIR)	575:612	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	13	14	with	biocompatible	1466:1478	arg1	potential					1493:1501	greater potential	1485:1501	greater potential to be used as chemoembolic material	1485:1537	Thus, ACMs were more biocompatible with greater potential to be used as chemoembolic material.
25311962	1	15	theme	different	161:169	arg1	acetylation					171:181	different acetylation	161:181	different acetylation using as a chemoembolic agent	161:211	The aim was to investigate the potential of chitosan microspheres (CMs) with different acetylation using as a chemoembolic agent.
25311962	11	16	theme	embryo	1287:1292	arg1	MEFs					1307:1310	MEFs	1307:1310	MEFs	1307:1310	In contrast, the relative growth rate of mouse embryo fibroblasts (MEFs) on CMs decreased gradually.
25311962	11	16	theme	embryo	1287:1292	arg1	fibroblasts					1294:1304	mouse embryo fibroblasts	1281:1304	mouse embryo fibroblasts (MEFs)	1281:1311	In contrast, the relative growth rate of mouse embryo fibroblasts (MEFs) on CMs decreased gradually.
25311962	2	17	theme	water-in-oil	260:271	arg1	method					308:313	water-in-oil (W/O) emulsification cross-linking method	260:313	water-in-oil (W/O) emulsification cross-linking method	260:313	Chitosan microspheres (CMs) were prepared via water-in-oil (W/O) emulsification cross-linking method, and acetylated chitosan microspheres (ACMs) were obtained by acetylation of CMs.
25311962	8	18	theme	time	1063:1066	arg1	%					1054:1054	<5%	1052:1054	<5% of the time	1052:1066	ACMs caused hemolysis less than CMs (<5% of the time).
25311962	8	18	theme	time	1063:1066	arg1	time					1063:1066	the time	1059:1066	the time	1059:1066	ACMs caused hemolysis less than CMs (<5% of the time).
25311962	6	19	theme	Protein	867:873	arg1	assays					886:891	Protein absorption assays	867:891	Protein absorption assays	867:891	Protein absorption assays suggested that albumin was more greatly adsorbed on CMs than on ACMs.
25311962	1	20	with	potential	115:123	arg1	acetylation					171:181	different acetylation	161:181	different acetylation using as a chemoembolic agent	161:211	The aim was to investigate the potential of chitosan microspheres (CMs) with different acetylation using as a chemoembolic agent.
25311962	3	21	theme	light	539:543	arg1	DLS					557:559	DLS	557:559	DLS	557:559	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	3	21	theme	light	539:543	arg1	scattering					545:554	dynamic laser light scattering	525:554	dynamic laser light scattering (DLS)	525:560	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	5	22	theme	pH	802:803	arg1	increase					790:797	the increase	786:797	the increase of pH (4.0-10.0)	786:814	The swelling rate (SR) of CMs decreased significantly with the increase of pH (4.0-10.0) but ACMs did not change under the same conditions.
25311962	7	23	theme	blood	992:996	arg1	clots					998:1002	more blood clots	987:1002	more blood clots	987:1002	Furthermore, CMs caused more blood clots than ACMs.
25311962	5	24	theme	same	850:853	arg1	conditions					855:864	the same conditions	846:864	the same conditions	846:864	The swelling rate (SR) of CMs decreased significantly with the increase of pH (4.0-10.0) but ACMs did not change under the same conditions.
25311962	0	25	theme	chitosan	28:35	arg1	microspheres					37:48	acetylated chitosan microspheres	17:48	acetylated chitosan microspheres	17:48	Investigation of acetylated chitosan microspheres as potential chemoembolic agents.
25311962	2	26	theme	cross-linking	294:306	arg1	method					308:313	water-in-oil (W/O) emulsification cross-linking method	260:313	water-in-oil (W/O) emulsification cross-linking method	260:313	Chitosan microspheres (CMs) were prepared via water-in-oil (W/O) emulsification cross-linking method, and acetylated chitosan microspheres (ACMs) were obtained by acetylation of CMs.
25311962	13	27	theme	chemoembolic	1517:1528	arg1	material					1530:1537	chemoembolic material	1517:1537	chemoembolic material	1517:1537	Thus, ACMs were more biocompatible with greater potential to be used as chemoembolic material.
25311962	0	28	theme	acetylated	17:26	arg1	microspheres					37:48	acetylated chitosan microspheres	17:48	acetylated chitosan microspheres	17:48	Investigation of acetylated chitosan microspheres as potential chemoembolic agents.
25311962	6	29	theme	absorption	875:884	arg1	assays					886:891	Protein absorption assays	867:891	Protein absorption assays	867:891	Protein absorption assays suggested that albumin was more greatly adsorbed on CMs than on ACMs.
25311962	2	30	theme	emulsification	279:292	arg1	method					308:313	water-in-oil (W/O) emulsification cross-linking method	260:313	water-in-oil (W/O) emulsification cross-linking method	260:313	Chitosan microspheres (CMs) were prepared via water-in-oil (W/O) emulsification cross-linking method, and acetylated chitosan microspheres (ACMs) were obtained by acetylation of CMs.
25311962	10	31	theme	attached	1186:1193	arg1	proliferation					1195:1207	less cell attached proliferation	1176:1207	less cell attached proliferation	1176:1207	Cytotoxicity tests indicated that ACMs initially had less cell attached proliferation but increased with incubation.
25311962	1	32	theme	chemoembolic	194:205	arg1	agent					207:211	a chemoembolic agent	192:211	a chemoembolic agent	192:211	The aim was to investigate the potential of chitosan microspheres (CMs) with different acetylation using as a chemoembolic agent.
25311962	3	33	theme	deacetylation	469:481	arg1	size					436:439	size	436:439	size	436:439	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	3	33	theme	deacetylation	469:481	arg1	morphology					424:433	the morphology	420:433	the morphology	420:433	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	3	33	theme	deacetylation	469:481	arg1	degrees					458:464	degrees	458:464	degrees	458:464	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	3	33	theme	deacetylation	469:481	arg1	composition					442:452	composition	442:452	composition	442:452	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	9	34	theme	more	1099:1102	arg1	hemocompatibility					1104:1120	more hemocompatibility	1099:1120	more hemocompatibility	1099:1120	Data indicated that ACMs had more hemocompatibility.
25311962	10	35	theme	cell	1181:1184	arg1	proliferation					1195:1207	less cell attached proliferation	1176:1207	less cell attached proliferation	1176:1207	Cytotoxicity tests indicated that ACMs initially had less cell attached proliferation but increased with incubation.
25311962	3	36	dep	transform	575:583	arg1	infrared					585:592	infrared	585:592	transform infrared spectrometer (FTIR)	575:612	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	0	37	theme	microspheres	37:48	arg1	Investigation					0:12	Investigation	0:12	Investigation of acetylated chitosan microspheres as potential chemoembolic agents.	0:82	Investigation of acetylated chitosan microspheres as potential chemoembolic agents.
25311962	5	38	theme	swelling	731:738	arg1	rate					740:743	The swelling rate	727:743	The swelling rate (SR) of CMs	727:755	The swelling rate (SR) of CMs decreased significantly with the increase of pH (4.0-10.0) but ACMs did not change under the same conditions.
25311962	5	38	theme	swelling	731:738	arg1	SR					746:747	SR	746:747	SR	746:747	The swelling rate (SR) of CMs decreased significantly with the increase of pH (4.0-10.0) but ACMs did not change under the same conditions.
25311962	2	39	theme	CMs	392:394	arg1	acetylation					377:387	acetylation	377:387	acetylation of CMs	377:394	Chitosan microspheres (CMs) were prepared via water-in-oil (W/O) emulsification cross-linking method, and acetylated chitosan microspheres (ACMs) were obtained by acetylation of CMs.
25311962	0	40	theme	chemoembolic	63:74	arg1	agents					76:81	potential chemoembolic agents	53:81	potential chemoembolic agents	53:81	Investigation of acetylated chitosan microspheres as potential chemoembolic agents.
25311962	0	41	theme	potential	53:61	arg1	agents					76:81	potential chemoembolic agents	53:81	potential chemoembolic agents	53:81	Investigation of acetylated chitosan microspheres as potential chemoembolic agents.
25311962	2	42	theme	Chitosan	214:221	arg1	CMs					237:239	CMs	237:239	CMs	237:239	Chitosan microspheres (CMs) were prepared via water-in-oil (W/O) emulsification cross-linking method, and acetylated chitosan microspheres (ACMs) were obtained by acetylation of CMs.
25311962	2	42	theme	Chitosan	214:221	arg1	microspheres					223:234	Chitosan microspheres	214:234	Chitosan microspheres (CMs)	214:240	Chitosan microspheres (CMs) were prepared via water-in-oil (W/O) emulsification cross-linking method, and acetylated chitosan microspheres (ACMs) were obtained by acetylation of CMs.
25311962	2	43	theme	chitosan	331:338	arg1	ACMs					354:357	ACMs	354:357	ACMs	354:357	Chitosan microspheres (CMs) were prepared via water-in-oil (W/O) emulsification cross-linking method, and acetylated chitosan microspheres (ACMs) were obtained by acetylation of CMs.
25311962	2	43	theme	chitosan	331:338	arg1	microspheres					340:351	acetylated chitosan microspheres	320:351	acetylated chitosan microspheres (ACMs)	320:358	Chitosan microspheres (CMs) were prepared via water-in-oil (W/O) emulsification cross-linking method, and acetylated chitosan microspheres (ACMs) were obtained by acetylation of CMs.
25311962	13	44	theme	greater	1485:1491	arg1	potential					1493:1501	greater potential	1485:1501	greater potential to be used as chemoembolic material	1485:1537	Thus, ACMs were more biocompatible with greater potential to be used as chemoembolic material.
25311962	4	45	contain	had	632:634	arg2	flexibility					672:682	good mechanical flexibility	656:682	good mechanical flexibility	656:682	All microspheres had smooth surfaces and good mechanical flexibility, and all could pass through a 5F catheter.
25311962	4	45	contain	had	632:634	arg1	microspheres					619:630	All microspheres	615:630	All microspheres	615:630	All microspheres had smooth surfaces and good mechanical flexibility, and all could pass through a 5F catheter.
25311962	4	45	contain	had	632:634	arg2	surfaces					643:650	smooth surfaces	636:650	smooth surfaces	636:650	All microspheres had smooth surfaces and good mechanical flexibility, and all could pass through a 5F catheter.
25311962	1	46	theme	chitosan	128:135	arg1	CMs					151:153	CMs	151:153	CMs	151:153	The aim was to investigate the potential of chitosan microspheres (CMs) with different acetylation using as a chemoembolic agent.
25311962	1	46	theme	chitosan	128:135	arg1	microspheres					137:148	chitosan microspheres	128:148	chitosan microspheres (CMs)	128:154	The aim was to investigate the potential of chitosan microspheres (CMs) with different acetylation using as a chemoembolic agent.
25311962	3	47	theme	dynamic	525:531	arg1	DLS					557:559	DLS	557:559	DLS	557:559	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	3	47	theme	dynamic	525:531	arg1	scattering					545:554	dynamic laser light scattering	525:554	dynamic laser light scattering (DLS)	525:560	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	11	48	from	rate	1273:1276	arg1	CMs					1316:1318	CMs	1316:1318	CMs	1316:1318	In contrast, the relative growth rate of mouse embryo fibroblasts (MEFs) on CMs decreased gradually.
25311962	1	49	theme	microspheres	137:148	arg1	potential					115:123	the potential	111:123	the potential of chitosan microspheres (CMs) with different acetylation using as a chemoembolic agent	111:211	The aim was to investigate the potential of chitosan microspheres (CMs) with different acetylation using as a chemoembolic agent.
25311962	2	50	theme	acetylated	320:329	arg1	ACMs					354:357	ACMs	354:357	ACMs	354:357	Chitosan microspheres (CMs) were prepared via water-in-oil (W/O) emulsification cross-linking method, and acetylated chitosan microspheres (ACMs) were obtained by acetylation of CMs.
25311962	2	50	theme	acetylated	320:329	arg1	microspheres					340:351	acetylated chitosan microspheres	320:351	acetylated chitosan microspheres (ACMs)	320:358	Chitosan microspheres (CMs) were prepared via water-in-oil (W/O) emulsification cross-linking method, and acetylated chitosan microspheres (ACMs) were obtained by acetylation of CMs.
25311962	8	51	theme	less	1037:1040	arg1	hemolysis					1027:1035	hemolysis	1027:1035	hemolysis less than CMs (<5% of the time)	1027:1067	ACMs caused hemolysis less than CMs (<5% of the time).
25311962	10	52	theme	Cytotoxicity	1123:1134	arg1	tests					1136:1140	Cytotoxicity tests	1123:1140	Cytotoxicity tests	1123:1140	Cytotoxicity tests indicated that ACMs initially had less cell attached proliferation but increased with incubation.
25311962	3	53	theme	electron	498:505	arg1	TEM					519:521	TEM	519:521	TEM	519:521	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	3	53	theme	electron	498:505	arg1	microscopy					507:516	scanning electron microscopy	489:516	scanning electron microscopy (TEM)	489:522	Next, we characterized the morphology, size, composition and degrees of deacetylation using scanning electron microscopy (TEM), dynamic laser light scattering (DLS), and Fourier transform infrared spectrometer (FTIR).
25311962	9	54	contain	had	1095:1097	arg2	hemocompatibility					1104:1120	more hemocompatibility	1099:1120	more hemocompatibility	1099:1120	Data indicated that ACMs had more hemocompatibility.
25311962	9	54	contain	had	1095:1097	arg1	ACMs					1090:1093	ACMs	1090:1093	ACMs	1090:1093	Data indicated that ACMs had more hemocompatibility.
25311962	5	55	theme	CMs	753:755	arg1	rate					740:743	The swelling rate	727:743	The swelling rate (SR) of CMs	727:755	The swelling rate (SR) of CMs decreased significantly with the increase of pH (4.0-10.0) but ACMs did not change under the same conditions.
25311962	5	55	theme	CMs	753:755	arg1	SR					746:747	SR	746:747	SR	746:747	The swelling rate (SR) of CMs decreased significantly with the increase of pH (4.0-10.0) but ACMs did not change under the same conditions.
25311962	4	56	theme	smooth	636:641	arg1	surfaces					643:650	smooth surfaces	636:650	smooth surfaces	636:650	All microspheres had smooth surfaces and good mechanical flexibility, and all could pass through a 5F catheter.
25311962	4	57	theme	mechanical	661:670	arg1	flexibility					672:682	good mechanical flexibility	656:682	good mechanical flexibility	656:682	All microspheres had smooth surfaces and good mechanical flexibility, and all could pass through a 5F catheter.
25921640	0	0	theme	delivery	108:115	arg1	potential					117:125	controlled delivery potential	97:125	controlled delivery potential	97:125	Electrophoretic deposition of antibiotic loaded PHBV microsphere-alginate composite coating with controlled delivery potential.
25921640	5	1	theme	co-deposition	903:915	arg1	mechanism					917:925	The co-deposition mechanism	899:925	The co-deposition mechanism	899:925	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	3	2	theme	increasing	566:575	arg1	concentrations					580:593	increasing MS concentrations	566:593	increasing MS concentrations	566:593	Linearly increased deposit mass with increasing MS concentrations was obtained, and MS were found to be homogeneously stabilized in the alginate matrix.
25921640	0	3	theme	controlled	97:106	arg1	potential					117:125	controlled delivery potential	97:125	controlled delivery potential	97:125	Electrophoretic deposition of antibiotic loaded PHBV microsphere-alginate composite coating with controlled delivery potential.
25921640	6	4	theme	inhibition	1185:1194	arg1	effects					1196:1202	excellent inhibition effects	1175:1202	excellent inhibition effects against E. coli (gram-negative bacteria)	1175:1243	Qualitative antibacterial tests indicated that MS containing coatings exhibit excellent inhibition effects against E. coli (gram-negative bacteria) after 1h of incubation.
25921640	3	5	theme	MS	577:578	arg1	concentrations					580:593	increasing MS concentrations	566:593	increasing MS concentrations	566:593	Linearly increased deposit mass with increasing MS concentrations was obtained, and MS were found to be homogeneously stabilized in the alginate matrix.
25921640	7	6	theme	promising	1367:1375	arg1	approach					1398:1405	a promising surface modification approach	1365:1405	a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules	1365:1471	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	6	7	theme	excellent	1175:1183	arg1	effects					1196:1202	excellent inhibition effects	1175:1202	excellent inhibition effects against E. coli (gram-negative bacteria)	1175:1243	Qualitative antibacterial tests indicated that MS containing coatings exhibit excellent inhibition effects against E. coli (gram-negative bacteria) after 1h of incubation.
25921640	4	8	theme	water	852:856	arg1	instruments					872:882	water contact angle instruments	852:882	water contact angle instruments	852:882	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	7	9	theme	modification	1385:1396	arg1	approach					1398:1405	a promising surface modification approach	1365:1405	a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules	1365:1471	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	4	10	theme	laser	829:833	arg1	profilometer					835:846	laser profilometer	829:846	laser profilometer	829:846	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	2	11	theme	different	341:349	arg1	concentrations					354:367	different MS concentrations	341:367	different MS concentrations	341:367	The composite coatings deposited from suspensions with different MS concentrations were produced in order to demonstrate the versatility of the proposed method for achieving functional coatings with tailored drug loading and release profiles.
25921640	7	12	theme	proposed	1273:1280	arg1	system					1290:1295	The proposed coating system	1269:1295	The proposed coating system combined with the simplicity of the EPD technique	1269:1345	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	6	13	theme	incubation	1257:1266	arg1	1h					1251:1252	1h	1251:1252	1h of incubation	1251:1266	Qualitative antibacterial tests indicated that MS containing coatings exhibit excellent inhibition effects against E. coli (gram-negative bacteria) after 1h of incubation.
25921640	4	14	dep	Fourier	781:787	arg1	transform					789:797	transform	789:797	transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively	789:896	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	7	15	theme	EPD	1333:1335	arg1	technique					1337:1345	the EPD technique	1329:1345	the EPD technique	1329:1345	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	0	16	with	coating	84:90	arg1	potential					117:125	controlled delivery potential	97:125	controlled delivery potential	97:125	Electrophoretic deposition of antibiotic loaded PHBV microsphere-alginate composite coating with controlled delivery potential.
25921640	5	17	theme	alginate-adsorbed	1040:1056	arg1	MS					1058:1059	alginate-adsorbed MS	1040:1059	alginate-adsorbed MS	1040:1059	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	5	18	theme	measurements	1004:1015	arg1	results					984:990	the results	980:990	the results of relevant measurements	980:1015	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	5	19	dep	alginate	1087:1094	arg1	ii					1066:1067	ii	1066:1067	ii	1066:1067	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	0	20	theme	microsphere-alginate	53:72	arg1	coating					84:90	microsphere-alginate composite coating	53:90	microsphere-alginate composite coating with controlled delivery potential	53:125	Electrophoretic deposition of antibiotic loaded PHBV microsphere-alginate composite coating with controlled delivery potential.
25921640	5	21	theme	MS	1058:1059	arg1	alginate					1087:1094	the non-adsorbed alginate	1070:1094	(ii) the non-adsorbed alginate	1065:1094	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	5	21	theme	MS	1058:1059	arg1	deposition					1026:1035	the deposition	1022:1035	(i) the deposition of alginate-adsorbed MS	1018:1059	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	5	21	theme	MS	1058:1059	arg1	processes					957:965	two separate processes	944:965	two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate	944:1094	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	2	22	theme	method	439:444	arg1	versatility					411:421	the versatility	407:421	the versatility of the proposed method for achieving functional coatings with tailored drug loading and release profiles	407:526	The composite coatings deposited from suspensions with different MS concentrations were produced in order to demonstrate the versatility of the proposed method for achieving functional coatings with tailored drug loading and release profiles.
25921640	3	23	with	mass	556:559	arg1	concentrations					580:593	increasing MS concentrations	566:593	increasing MS concentrations	566:593	Linearly increased deposit mass with increasing MS concentrations was obtained, and MS were found to be homogeneously stabilized in the alginate matrix.
25921640	7	24	theme	technique	1337:1345	arg1	simplicity					1315:1324	the simplicity	1311:1324	the simplicity of the EPD technique	1311:1345	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	4	25	theme	FTIR	809:812	arg1	spectroscopy					815:826	(FTIR) spectroscopy	808:826	(FTIR) spectroscopy	808:826	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	2	26	theme	proposed	430:437	arg1	method					439:444	the proposed method	426:444	the proposed method for achieving functional coatings with tailored drug loading and release profiles	426:526	The composite coatings deposited from suspensions with different MS concentrations were produced in order to demonstrate the versatility of the proposed method for achieving functional coatings with tailored drug loading and release profiles.
25921640	0	27	theme	Electrophoretic	0:14	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of antibiotic	0:39	Electrophoretic deposition of antibiotic loaded PHBV microsphere-alginate composite coating with controlled delivery potential.
25921640	4	28	dep	transform	789:797	arg1	infrared					799:806	infrared	799:806	transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively	789:896	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	7	29	theme	drug	1446:1449	arg1	delivery					1434:1441	the controlled in situ delivery	1411:1441	the controlled in situ delivery of drug or other biomolecules	1411:1471	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	1	30	theme	antibiotic-loaded	213:229	arg1	coatings					276:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	Electrophoretic deposition (EPD) technique has been developed for the fabrication of antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings.
25921640	0	31	theme	antibiotic	30:39	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of antibiotic	0:39	Electrophoretic deposition of antibiotic loaded PHBV microsphere-alginate composite coating with controlled delivery potential.
25921640	5	32	dep	deposition	1026:1035	arg1	i					1019:1019	i	1019:1019	i	1019:1019	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	7	33	dep	in	1426:1427	arg1	situ					1429:1432	situ	1429:1432	situ	1429:1432	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	1	34	theme	Electrophoretic	128:142	arg1	technique					161:169	Electrophoretic deposition (EPD) technique	128:169	Electrophoretic deposition (EPD) technique	128:169	Electrophoretic deposition (EPD) technique has been developed for the fabrication of antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings.
25921640	1	35	theme	PHBV	231:234	arg1	coatings					276:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	Electrophoretic deposition (EPD) technique has been developed for the fabrication of antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings.
25921640	2	36	theme	tailored	485:492	arg1	loading					499:505	tailored drug loading	485:505	tailored drug loading	485:505	The composite coatings deposited from suspensions with different MS concentrations were produced in order to demonstrate the versatility of the proposed method for achieving functional coatings with tailored drug loading and release profiles.
25921640	5	37	dep	processes	957:965	arg1	alginate					1087:1094	the non-adsorbed alginate	1070:1094	(ii) the non-adsorbed alginate	1065:1094	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	5	37	dep	processes	957:965	arg1	deposition					1026:1035	the deposition	1022:1035	(i) the deposition of alginate-adsorbed MS	1018:1059	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	5	37	dep	processes	957:965	arg1	processes					957:965	two separate processes	944:965	two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate	944:1094	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	1	38	theme	deposition	144:153	arg1	technique					161:169	Electrophoretic deposition (EPD) technique	128:169	Electrophoretic deposition (EPD) technique	128:169	Electrophoretic deposition (EPD) technique has been developed for the fabrication of antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings.
25921640	1	39	theme	microsphere	236:246	arg1	coatings					276:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	Electrophoretic deposition (EPD) technique has been developed for the fabrication of antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings.
25921640	7	40	theme	surface	1377:1383	arg1	approach					1398:1405	a promising surface modification approach	1365:1405	a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules	1365:1471	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	5	41	theme	relevant	995:1002	arg1	measurements					1004:1015	relevant measurements	995:1015	relevant measurements	995:1015	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	6	42	theme	antibacterial	1109:1121	arg1	tests					1123:1127	Qualitative antibacterial tests	1097:1127	Qualitative antibacterial tests	1097:1127	Qualitative antibacterial tests indicated that MS containing coatings exhibit excellent inhibition effects against E. coli (gram-negative bacteria) after 1h of incubation.
25921640	7	43	theme	biomolecules	1460:1471	arg1	delivery					1434:1441	the controlled in situ delivery	1411:1441	the controlled in situ delivery of drug or other biomolecules	1411:1471	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	2	44	theme	functional	460:469	arg1	coatings					471:478	functional coatings	460:478	functional coatings	460:478	The composite coatings deposited from suspensions with different MS concentrations were produced in order to demonstrate the versatility of the proposed method for achieving functional coatings with tailored drug loading and release profiles.
25921640	6	45	theme	MS	1144:1145	arg1	coatings					1158:1165	MS containing coatings	1144:1165	MS containing coatings	1144:1165	Qualitative antibacterial tests indicated that MS containing coatings exhibit excellent inhibition effects against E. coli (gram-negative bacteria) after 1h of incubation.
25921640	5	46	theme	separate	948:955	arg1	alginate					1087:1094	the non-adsorbed alginate	1070:1094	(ii) the non-adsorbed alginate	1065:1094	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	5	46	theme	separate	948:955	arg1	deposition					1026:1035	the deposition	1022:1035	(i) the deposition of alginate-adsorbed MS	1018:1059	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	5	46	theme	separate	948:955	arg1	processes					957:965	two separate processes	944:965	two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate	944:1094	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	7	47	theme	controlled	1415:1424	arg1	delivery					1434:1441	the controlled in situ delivery	1411:1441	the controlled in situ delivery of drug or other biomolecules	1411:1471	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	2	48	theme	drug	494:497	arg1	loading					499:505	tailored drug loading	485:505	tailored drug loading	485:505	The composite coatings deposited from suspensions with different MS concentrations were produced in order to demonstrate the versatility of the proposed method for achieving functional coatings with tailored drug loading and release profiles.
25921640	1	49	theme	MS	249:250	arg1	coatings					276:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	Electrophoretic deposition (EPD) technique has been developed for the fabrication of antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings.
25921640	0	50	theme	composite	74:82	arg1	coating					84:90	microsphere-alginate composite coating	53:90	microsphere-alginate composite coating with controlled delivery potential	53:125	Electrophoretic deposition of antibiotic loaded PHBV microsphere-alginate composite coating with controlled delivery potential.
25921640	7	51	theme	coating	1282:1288	arg1	system					1290:1295	The proposed coating system	1269:1295	The proposed coating system combined with the simplicity of the EPD technique	1269:1345	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	6	52	theme	Qualitative	1097:1107	arg1	tests					1123:1127	Qualitative antibacterial tests	1097:1127	Qualitative antibacterial tests	1097:1127	Qualitative antibacterial tests indicated that MS containing coatings exhibit excellent inhibition effects against E. coli (gram-negative bacteria) after 1h of incubation.
25921640	2	53	theme	release	511:517	arg1	profiles					519:526	release profiles	511:526	release profiles	511:526	The composite coatings deposited from suspensions with different MS concentrations were produced in order to demonstrate the versatility of the proposed method for achieving functional coatings with tailored drug loading and release profiles.
25921640	7	54	theme	other	1454:1458	arg1	biomolecules					1460:1471	other biomolecules	1454:1471	other biomolecules	1454:1471	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	7	55	theme	in	1426:1427	arg1	delivery					1434:1441	the controlled in situ delivery	1411:1441	the controlled in situ delivery of drug or other biomolecules	1411:1471	The proposed coating system combined with the simplicity of the EPD technique can be considered a promising surface modification approach for the controlled in situ delivery of drug or other biomolecules.
25921640	1	56	theme	EPD	156:158	arg1	technique					161:169	Electrophoretic deposition (EPD) technique	128:169	Electrophoretic deposition (EPD) technique	128:169	Electrophoretic deposition (EPD) technique has been developed for the fabrication of antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings.
25921640	4	57	theme	angle	866:870	arg1	instruments					872:882	water contact angle instruments	852:882	water contact angle instruments	852:882	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	4	58	theme	contact	858:864	arg1	instruments					872:882	water contact angle instruments	852:882	water contact angle instruments	852:882	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	5	59	theme	non-adsorbed	1074:1085	arg1	alginate					1087:1094	the non-adsorbed alginate	1070:1094	(ii) the non-adsorbed alginate	1065:1094	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	5	59	theme	non-adsorbed	1074:1085	arg1	processes					957:965	two separate processes	944:965	two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate	944:1094	The co-deposition mechanism was described by two separate processes according to the results of relevant measurements: (i) the deposition of alginate-adsorbed MS and (ii) the non-adsorbed alginate.
25921640	3	60	theme	increased	538:546	arg1	mass					556:559	Linearly increased deposit mass	529:559	Linearly increased deposit mass with increasing MS concentrations	529:593	Linearly increased deposit mass with increasing MS concentrations was obtained, and MS were found to be homogeneously stabilized in the alginate matrix.
25921640	2	61	theme	MS	351:352	arg1	concentrations					354:367	different MS concentrations	341:367	different MS concentrations	341:367	The composite coatings deposited from suspensions with different MS concentrations were produced in order to demonstrate the versatility of the proposed method for achieving functional coatings with tailored drug loading and release profiles.
25921640	2	62	theme	composite	290:298	arg1	coatings					300:307	The composite coatings	286:307	The composite coatings deposited from suspensions with different MS concentrations	286:367	The composite coatings deposited from suspensions with different MS concentrations were produced in order to demonstrate the versatility of the proposed method for achieving functional coatings with tailored drug loading and release profiles.
25921640	3	63	theme	deposit	548:554	arg1	mass					556:559	Linearly increased deposit mass	529:559	Linearly increased deposit mass with increasing MS concentrations	529:593	Linearly increased deposit mass with increasing MS concentrations was obtained, and MS were found to be homogeneously stabilized in the alginate matrix.
25921640	1	64	theme	-alginate	252:260	arg1	coatings					276:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	Electrophoretic deposition (EPD) technique has been developed for the fabrication of antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings.
25921640	4	65	theme	surface	704:710	arg1	roughness					712:720	surface roughness	704:720	surface roughness	704:720	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	3	66	theme	alginate	665:672	arg1	matrix					674:679	the alginate matrix	661:679	the alginate matrix	661:679	Linearly increased deposit mass with increasing MS concentrations was obtained, and MS were found to be homogeneously stabilized in the alginate matrix.
25921640	4	67	theme	Chemical	682:689	arg1	composition					691:701	Chemical composition	682:701	Chemical composition	682:701	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	4	68	theme	coatings	755:762	arg1	wettability					726:736	wettability	726:736	wettability	726:736	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	4	68	theme	coatings	755:762	arg1	composition					691:701	Chemical composition	682:701	Chemical composition	682:701	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	4	68	theme	coatings	755:762	arg1	roughness					712:720	surface roughness	704:720	surface roughness	704:720	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	6	69	theme	containing	1147:1156	arg1	coatings					1158:1165	MS containing coatings	1144:1165	MS containing coatings	1144:1165	Qualitative antibacterial tests indicated that MS containing coatings exhibit excellent inhibition effects against E. coli (gram-negative bacteria) after 1h of incubation.
25921640	1	70	theme	antibacterial	262:274	arg1	coatings					276:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	213:283	Electrophoretic deposition (EPD) technique has been developed for the fabrication of antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings.
25921640	6	71	theme	gram-negative	1221:1233	arg1	coli					1215:1218	E. coli	1212:1218	E. coli (gram-negative bacteria)	1212:1243	Qualitative antibacterial tests indicated that MS containing coatings exhibit excellent inhibition effects against E. coli (gram-negative bacteria) after 1h of incubation.
25921640	6	71	theme	gram-negative	1221:1233	arg1	bacteria					1235:1242	gram-negative bacteria	1221:1242	gram-negative bacteria	1221:1242	Qualitative antibacterial tests indicated that MS containing coatings exhibit excellent inhibition effects against E. coli (gram-negative bacteria) after 1h of incubation.
25921640	2	72	with	suspensions	324:334	arg1	concentrations					354:367	different MS concentrations	341:367	different MS concentrations	341:367	The composite coatings deposited from suspensions with different MS concentrations were produced in order to demonstrate the versatility of the proposed method for achieving functional coatings with tailored drug loading and release profiles.
25921640	4	73	theme	deposited	745:753	arg1	coatings					755:762	the deposited coatings	741:762	the deposited coatings	741:762	Chemical composition, surface roughness and wettability of the deposited coatings were measured by Fourier transform infrared (FTIR) spectroscopy, laser profilometer and water contact angle instruments, respectively.
25921640	1	74	theme	coatings	276:283	arg1	fabrication					198:208	the fabrication	194:208	the fabrication of antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings	194:283	Electrophoretic deposition (EPD) technique has been developed for the fabrication of antibiotic-loaded PHBV microsphere (MS)-alginate antibacterial coatings.
25273383	4	0	theme	O	939:939	arg1	polysaccharide					941:954	smooth O polysaccharide	932:954	smooth O polysaccharide	932:954	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	2	1	theme	type	383:386	arg1	Sp7					395:397	A. brasilense type strain Sp7	369:397	A. brasilense type strain Sp7	369:397	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	4	2	theme	EO	750:751	arg1	signal					753:758	the strongest EO signal	736:758	the strongest EO signal	736:758	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	4	3	theme	smooth	932:937	arg1	polysaccharide					941:954	smooth O polysaccharide	932:954	smooth O polysaccharide	932:954	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	6	4	theme	antigenic	1206:1214	arg1	composition					1216:1226	the antigenic composition	1202:1226	the antigenic composition of the bacterial surface	1202:1251	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	2	5	theme	brasilense	372:381	arg1	Sp7					395:397	A. brasilense type strain Sp7	369:397	A. brasilense type strain Sp7	369:397	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	6	6	from	importance	1095:1104	arg1	biotechnology					1182:1194	microbial biotechnology	1172:1194	microbial biotechnology	1172:1194	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	4	7	theme	strongest	740:748	arg1	signal					753:758	the strongest EO signal	736:758	the strongest EO signal	736:758	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	2	8	used	used	305:308	arg2	we					302:303	we	302:303	we	302:303	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	4	9	theme	culture	720:726	arg1	growth					728:733	culture growth	720:733	culture growth	720:733	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	2	10	theme	complete	338:345	arg1	lipopolysaccharide					347:364	the complete lipopolysaccharide	334:364	the complete lipopolysaccharide of A. brasilense type strain Sp7	334:397	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	1	11	theme	carbohydrate	128:139	arg1	epitopes					141:148	Azospirillum brasilense carbohydrate epitopes	104:148	Azospirillum brasilense carbohydrate epitopes	104:148	The exposure of Azospirillum brasilense carbohydrate epitopes was investigated by electro-optical analysis of bacterial cell suspensions.
25273383	4	12	theme	rough	814:818	arg1	polysaccharide					822:835	rough O polysaccharide	814:835	rough O polysaccharide (approximately 46% greater than the control)	814:880	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	4	13	dep	observed	764:771	arg1	whereas					883:889	whereas	883:889	whereas	883:889	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	1	14	theme	bacterial	198:206	arg1	suspensions					213:223	bacterial cell suspensions	198:223	bacterial cell suspensions	198:223	The exposure of Azospirillum brasilense carbohydrate epitopes was investigated by electro-optical analysis of bacterial cell suspensions.
25273383	2	15	from	changes	235:241	arg1	properties					271:280	the electro-optical (EO) properties	246:280	the electro-optical (EO) properties of the suspensions	246:299	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	2	16	theme	suspensions	289:299	arg1	properties					271:280	the electro-optical (EO) properties	246:280	the electro-optical (EO) properties of the suspensions	246:299	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	3	17	theme	growth	492:497	arg1	hr					478:479	18 hr	475:479	18 hr of culture growth	475:497	After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 20% lower than that of the suspension treated with antibodies to complete lipopolysaccharide (control).
25273383	1	18	theme	cell	208:211	arg1	suspensions					213:223	bacterial cell suspensions	198:223	bacterial cell suspensions	198:223	The exposure of Azospirillum brasilense carbohydrate epitopes was investigated by electro-optical analysis of bacterial cell suspensions.
25273383	2	19	theme	Sp7	464:466	arg1	smooth					426:431	the smooth and rough O polysaccharides	422:459	smooth	426:431	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	2	19	theme	Sp7	464:466	arg1	polysaccharides					445:459	the smooth and rough O polysaccharides	422:459	polysaccharides	445:459	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	6	20	theme	study	1080:1084	arg1	results					1065:1071	The results	1061:1071	The results of the study	1061:1084	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	4	21	dep	lower	969:973	arg1	%					992:992	approximately 23%	976:992	approximately 23% of the control	976:1007	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	4	21	dep	lower	969:973	arg1	control					1001:1007	the control	997:1007	the control	997:1007	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	3	22	theme	smooth	559:564	arg1	polysaccharide					568:581	smooth O polysaccharide	559:581	smooth O polysaccharide	559:581	After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 20% lower than that of the suspension treated with antibodies to complete lipopolysaccharide (control).
25273383	6	23	theme	bacterial	1235:1243	arg1	surface					1245:1251	the bacterial surface	1231:1251	the bacterial surface	1231:1251	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	1	24	theme	suspensions	213:223	arg1	analysis					186:193	electro-optical analysis	170:193	electro-optical analysis of bacterial cell suspensions	170:223	The exposure of Azospirillum brasilense carbohydrate epitopes was investigated by electro-optical analysis of bacterial cell suspensions.
25273383	0	25	theme	Electro-optical	0:14	arg1	study					16:20	Electro-optical study	0:20	Electro-optical study of the exposure of Azospirillum	0:52	Electro-optical study of the exposure of Azospirillum brasilense carbohydrate epitopes.
25273383	4	26	theme	O	820:820	arg1	polysaccharide					822:835	rough O polysaccharide	814:835	rough O polysaccharide (approximately 46% greater than the control)	814:880	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	3	27	theme	O	566:566	arg1	polysaccharide					568:581	smooth O polysaccharide	559:581	smooth O polysaccharide	559:581	After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 20% lower than that of the suspension treated with antibodies to complete lipopolysaccharide (control).
25273383	6	28	theme	changes	1134:1140	arg1	evaluation					1120:1129	the rapid evaluation	1110:1129	the rapid evaluation of changes in lipopolysaccharide form	1110:1167	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	1	29	theme	Azospirillum	104:115	arg1	epitopes					141:148	Azospirillum brasilense carbohydrate epitopes	104:148	Azospirillum brasilense carbohydrate epitopes	104:148	The exposure of Azospirillum brasilense carbohydrate epitopes was investigated by electro-optical analysis of bacterial cell suspensions.
25273383	2	30	theme	rough	437:441	arg1	polysaccharides					445:459	the smooth and rough O polysaccharides	422:459	polysaccharides	445:459	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	2	31	theme	O	443:443	arg1	polysaccharides					445:459	the smooth and rough O polysaccharides	422:459	polysaccharides	445:459	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	1	32	theme	brasilense	117:126	arg1	epitopes					141:148	Azospirillum brasilense carbohydrate epitopes	104:148	Azospirillum brasilense carbohydrate epitopes	104:148	The exposure of Azospirillum brasilense carbohydrate epitopes was investigated by electro-optical analysis of bacterial cell suspensions.
25273383	6	33	theme	close	1262:1266	arg1	control					1268:1274	close control	1262:1274	close control	1262:1274	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	0	34	theme	exposure	29:36	arg1	study					16:20	Electro-optical study	0:20	Electro-optical study of the exposure of Azospirillum	0:52	Electro-optical study of the exposure of Azospirillum brasilense carbohydrate epitopes.
25273383	6	35	theme	lipopolysaccharide	1145:1162	arg1	form					1164:1167	lipopolysaccharide form	1145:1167	lipopolysaccharide form	1145:1167	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	6	36	theme	surface	1245:1251	arg1	composition					1216:1226	the antigenic composition	1202:1226	the antigenic composition of the bacterial surface	1202:1251	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	2	37	theme	electro-optical	250:264	arg1	properties					271:280	the electro-optical (EO) properties	246:280	the electro-optical (EO) properties of the suspensions	246:299	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	2	38	theme	A.	369:370	arg1	Sp7					395:397	A. brasilense type strain Sp7	369:397	A. brasilense type strain Sp7	369:397	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	0	39	theme	Azospirillum	41:52	arg1	exposure					29:36	the exposure	25:36	the exposure of Azospirillum	25:52	Electro-optical study of the exposure of Azospirillum brasilense carbohydrate epitopes.
25273383	4	40	theme	greater	856:862	arg1	%					854:854	approximately 46%	838:854	approximately 46% greater than the control	838:879	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	4	41	dep	polysaccharide	822:835	arg1	%					854:854	approximately 46%	838:854	approximately 46% greater than the control	838:879	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	3	42	theme	EO	504:505	arg1	lower					605:609	lower	605:609	lower	605:609	After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 20% lower than that of the suspension treated with antibodies to complete lipopolysaccharide (control).
25273383	3	42	theme	EO	504:505	arg1	signal					507:512	the EO signal	500:512	the EO signal of the suspension treated with antibodies to smooth O polysaccharide	500:581	After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 20% lower than that of the suspension treated with antibodies to complete lipopolysaccharide (control).
25273383	1	43	theme	epitopes	141:148	arg1	exposure					92:99	The exposure	88:99	The exposure of Azospirillum brasilense carbohydrate epitopes	88:148	The exposure of Azospirillum brasilense carbohydrate epitopes was investigated by electro-optical analysis of bacterial cell suspensions.
25273383	3	44	theme	complete	666:673	arg1	control					695:701	control	695:701	control	695:701	After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 20% lower than that of the suspension treated with antibodies to complete lipopolysaccharide (control).
25273383	3	44	theme	complete	666:673	arg1	lipopolysaccharide					675:692	complete lipopolysaccharide	666:692	complete lipopolysaccharide (control)	666:702	After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 20% lower than that of the suspension treated with antibodies to complete lipopolysaccharide (control).
25273383	6	45	theme	microbial	1172:1180	arg1	biotechnology					1182:1194	microbial biotechnology	1172:1194	microbial biotechnology	1172:1194	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	4	46	theme	control	1001:1007	arg1	%					992:992	approximately 23%	976:992	approximately 23% of the control	976:1007	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	4	46	theme	control	1001:1007	arg1	control					1001:1007	the control	997:1007	the control	997:1007	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	3	47	theme	suspension	521:530	arg1	lower					605:609	lower	605:609	lower	605:609	After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 20% lower than that of the suspension treated with antibodies to complete lipopolysaccharide (control).
25273383	3	47	theme	suspension	521:530	arg1	signal					507:512	the EO signal	500:512	the EO signal of the suspension treated with antibodies to smooth O polysaccharide	500:581	After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 20% lower than that of the suspension treated with antibodies to complete lipopolysaccharide (control).
25273383	4	48	theme	growth	728:733	arg1	hr					714:715	72 hr	711:715	72 hr of culture growth	711:733	After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control).
25273383	1	49	theme	electro-optical	170:184	arg1	analysis					186:193	electro-optical analysis	170:193	electro-optical analysis of bacterial cell suspensions	170:223	The exposure of Azospirillum brasilense carbohydrate epitopes was investigated by electro-optical analysis of bacterial cell suspensions.
25273383	6	50	from	form	1164:1167	arg1	evaluation					1120:1129	the rapid evaluation	1110:1129	the rapid evaluation of changes in lipopolysaccharide form	1110:1167	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	6	51	contain	have	1090:1093	arg1	results					1065:1071	The results	1061:1071	The results of the study	1061:1084	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	6	51	contain	have	1090:1093	arg2	importance					1095:1104	importance	1095:1104	importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology	1095:1194	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	6	52	from	evaluation	1120:1129	arg1	form					1164:1167	lipopolysaccharide form	1145:1167	lipopolysaccharide form	1145:1167	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	6	53	theme	rapid	1114:1118	arg1	evaluation					1120:1129	the rapid evaluation	1110:1129	the rapid evaluation of changes in lipopolysaccharide form	1110:1167	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	2	54	dep	electro-optical	250:264	arg1	EO					267:268	EO	267:268	EO	267:268	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	2	55	theme	Sp7	395:397	arg1	lipopolysaccharide					347:364	the complete lipopolysaccharide	334:364	the complete lipopolysaccharide of A. brasilense type strain Sp7	334:397	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
25273383	3	56	theme	culture	484:490	arg1	growth					492:497	culture growth	484:497	culture growth	484:497	After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 20% lower than that of the suspension treated with antibodies to complete lipopolysaccharide (control).
25273383	0	57	theme	carbohydrate	65:76	arg1	epitopes					78:85	carbohydrate epitopes	65:85	carbohydrate epitopes	65:85	Electro-optical study of the exposure of Azospirillum brasilense carbohydrate epitopes.
25273383	5	58	theme	electron	1040:1047	arg1	microscopy					1049:1058	electron microscopy	1040:1058	electron microscopy	1040:1058	These data were confirmed by electron microscopy.
25273383	6	59	from	changes	1134:1140	arg1	form					1164:1167	lipopolysaccharide form	1145:1167	lipopolysaccharide form	1145:1167	The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
25273383	2	60	theme	strain	388:393	arg1	Sp7					395:397	A. brasilense type strain Sp7	369:397	A. brasilense type strain Sp7	369:397	To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7.
28406056	5	0	theme	sources	910:916	arg1	rate					885:888	very fast adsorption rate	864:888	very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS)	864:933	Adsorption kinetics indicated very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS) and the adsorption process was well described by the pseudo-second-order kinetic model.
28406056	7	1	theme	equilibrium	1160:1170	arg1	data					1172:1175	The equilibrium data	1156:1175	The equilibrium data	1156:1175	The equilibrium data were described by the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions.
28406056	1	2	theme	present	168:174	arg1	study					176:180	the present study	164:180	the present study	164:180	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	1	3	theme	sodium	241:246	arg1	SLS					264:266	SLS	264:266	SLS	264:266	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	1	3	theme	sodium	241:246	arg1	sulfate					255:261	sodium lauryl sulfate	241:261	sodium lauryl sulfate (SLS)	241:267	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	0	4	theme	removal	89:95	arg1	study					72:76	the study	68:76	the study of sulfate removal	68:95	Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite microfibrillated cellulose composite.
28406056	5	5	theme	pseudo-second-order	988:1006	arg1	model					1016:1020	the pseudo-second-order kinetic model	984:1020	the pseudo-second-order kinetic model	984:1020	Adsorption kinetics indicated very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS) and the adsorption process was well described by the pseudo-second-order kinetic model.
28406056	1	6	theme	lauryl	248:253	arg1	SLS					264:266	SLS	264:266	SLS	264:266	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	1	6	theme	lauryl	248:253	arg1	sulfate					255:261	sodium lauryl sulfate	241:261	sodium lauryl sulfate (SLS)	241:267	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	0	7	theme	sulfate	81:87	arg1	removal					89:95	sulfate removal	81:95	sulfate removal	81:95	Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite microfibrillated cellulose composite.
28406056	1	8	theme	aqueous	350:356	arg1	solution					358:365	the aqueous solution	346:365	the aqueous solution	346:365	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	7	9	theme	isotherm	1253:1260	arg1	models					1262:1267	the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models	1195:1267	the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions	1195:1304	The equilibrium data were described by the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions.
28406056	6	10	theme	SLS	1112:1114	arg1	sulfates					1100:1107	sulfates	1100:1107	sulfates of SLS and 7.35 mg g-1 for sulfates of Na2SO4	1100:1153	Experimental maximum adsorption capacities were found to be 34.53 mg g-1 for sulfates of SLS and 7.35 mg g-1 for sulfates of Na2SO4.
28406056	1	11	theme	sulfate	255:261	arg1	sulfates					201:208	sulfates	201:208	sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS)	201:267	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	0	12	theme	calcium	100:106	arg1	hydroxyapatite					108:121	calcium hydroxyapatite	100:121	calcium hydroxyapatite	100:121	Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite microfibrillated cellulose composite.
28406056	7	13	theme	error	1290:1294	arg1	functions					1296:1304	five different error functions	1275:1304	five different error functions	1275:1304	The equilibrium data were described by the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions.
28406056	4	14	theme	contact	670:676	arg1	time					678:681	contact time	670:681	contact time	670:681	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	2	15	theme	electron	463:470	arg1	microscope					472:481	scanning electron microscope	454:481	scanning electron microscope	454:481	The adsorbent was characterized using elemental analysis, Fourier transform infrared, scanning electron microscope and elemental analysis in order to gain the information on its structure and physico-chemical properties.
28406056	2	16	dep	transform	434:442	arg1	infrared					444:451	infrared	444:451	transform infrared	434:451	The adsorbent was characterized using elemental analysis, Fourier transform infrared, scanning electron microscope and elemental analysis in order to gain the information on its structure and physico-chemical properties.
28406056	7	17	theme	Sips	1209:1212	arg1	models					1262:1267	the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models	1195:1267	the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions	1195:1304	The equilibrium data were described by the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions.
28406056	7	18	theme	Langmuir	1199:1206	arg1	models					1262:1267	the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models	1195:1267	the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions	1195:1304	The equilibrium data were described by the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions.
28406056	2	19	theme	scanning	454:461	arg1	microscope					472:481	scanning electron microscope	454:481	scanning electron microscope	454:481	The adsorbent was characterized using elemental analysis, Fourier transform infrared, scanning electron microscope and elemental analysis in order to gain the information on its structure and physico-chemical properties.
28406056	4	20	theme	competing	739:747	arg1	anions					749:754	competing anions	739:754	competing anions	739:754	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	5	21	dep	sources	910:916	arg1	sources					910:916	both sources	905:916	both sources (Na2SO4 and SLS)	905:933	Adsorption kinetics indicated very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS) and the adsorption process was well described by the pseudo-second-order kinetic model.
28406056	5	21	dep	sources	910:916	arg1	SLS					930:932	SLS	930:932	SLS	930:932	Adsorption kinetics indicated very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS) and the adsorption process was well described by the pseudo-second-order kinetic model.
28406056	5	21	dep	sources	910:916	arg1	Na2SO4					919:924	Na2SO4	919:924	Na2SO4	919:924	Adsorption kinetics indicated very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS) and the adsorption process was well described by the pseudo-second-order kinetic model.
28406056	2	22	from	information	527:537	arg1	structure					546:554	its structure	542:554	its structure	542:554	The adsorbent was characterized using elemental analysis, Fourier transform infrared, scanning electron microscope and elemental analysis in order to gain the information on its structure and physico-chemical properties.
28406056	2	22	from	information	527:537	arg1	properties					577:586	physico-chemical properties	560:586	physico-chemical properties	560:586	The adsorbent was characterized using elemental analysis, Fourier transform infrared, scanning electron microscope and elemental analysis in order to gain the information on its structure and physico-chemical properties.
28406056	5	23	theme	adsorption	874:883	arg1	rate					885:888	very fast adsorption rate	864:888	very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS)	864:933	Adsorption kinetics indicated very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS) and the adsorption process was well described by the pseudo-second-order kinetic model.
28406056	3	24	theme	adsorption	593:602	arg1	studies					604:610	The adsorption studies	589:610	The adsorption studies	589:610	The adsorption studies were conducted in batch mode.
28406056	0	25	theme	equilibrium	25:35	arg1	models					37:42	adsorption equilibrium models	14:42	adsorption equilibrium models	14:42	Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite microfibrillated cellulose composite.
28406056	5	26	theme	kinetic	1008:1014	arg1	model					1016:1020	the pseudo-second-order kinetic model	984:1020	the pseudo-second-order kinetic model	984:1020	Adsorption kinetics indicated very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS) and the adsorption process was well described by the pseudo-second-order kinetic model.
28406056	2	27	theme	physico-chemical	560:575	arg1	properties					577:586	physico-chemical properties	560:586	physico-chemical properties	560:586	The adsorbent was characterized using elemental analysis, Fourier transform infrared, scanning electron microscope and elemental analysis in order to gain the information on its structure and physico-chemical properties.
28406056	5	28	theme	adsorption	943:952	arg1	process					954:960	the adsorption process	939:960	the adsorption process	939:960	Adsorption kinetics indicated very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS) and the adsorption process was well described by the pseudo-second-order kinetic model.
28406056	0	29	theme	adsorption	14:23	arg1	models					37:42	adsorption equilibrium models	14:42	adsorption equilibrium models	14:42	Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite microfibrillated cellulose composite.
28406056	4	30	theme	anions	749:754	arg1	concentration					696:708	the initial concentration	684:708	the initial concentration of sulfate	684:719	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	4	30	theme	anions	749:754	arg1	effects					646:652	The effects	642:652	The effects of solution pH	642:667	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	4	30	theme	anions	749:754	arg1	time					678:681	contact time	670:681	contact time	670:681	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	4	30	theme	anions	749:754	arg1	effect					729:734	the effect	725:734	the effect of competing anions	725:754	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	1	31	theme	sulfates	201:208	arg1	adsorption					187:196	the adsorption	183:196	the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose	183:329	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	2	32	theme	elemental	487:495	arg1	analysis					497:504	elemental analysis	487:504	elemental analysis	487:504	The adsorbent was characterized using elemental analysis, Fourier transform infrared, scanning electron microscope and elemental analysis in order to gain the information on its structure and physico-chemical properties.
28406056	4	33	theme	solution	657:664	arg1	pH					666:667	solution pH	657:667	solution pH	657:667	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	4	34	theme	pH	666:667	arg1	concentration					696:708	the initial concentration	684:708	the initial concentration of sulfate	684:719	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	4	34	theme	pH	666:667	arg1	effects					646:652	The effects	642:652	The effects of solution pH	642:667	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	4	34	theme	pH	666:667	arg1	time					678:681	contact time	670:681	contact time	670:681	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	4	34	theme	pH	666:667	arg1	effect					729:734	the effect	725:734	the effect of competing anions	725:754	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	0	35	theme	models	37:42	arg1	Comparison					0:9	Comparison	0:9	Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite	0:121	Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite microfibrillated cellulose composite.
28406056	1	36	theme	calcium	272:278	arg1	cellulose					321:329	calcium hydroxyapatite-modified microfibrillated cellulose	272:329	calcium hydroxyapatite-modified microfibrillated cellulose	272:329	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	0	37	theme	cellulose	140:148	arg1	composite					150:158	cellulose composite	140:158	cellulose composite	140:158	Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite microfibrillated cellulose composite.
28406056	4	38	theme	sulfate	817:823	arg1	removal					825:831	sulfate removal	817:831	sulfate removal	817:831	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	5	39	theme	fast	869:872	arg1	rate					885:888	very fast adsorption rate	864:888	very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS)	864:933	Adsorption kinetics indicated very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS) and the adsorption process was well described by the pseudo-second-order kinetic model.
28406056	1	40	theme	sodium	213:218	arg1	Na2SO4					229:234	Na2SO4	229:234	Na2SO4	229:234	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	1	40	theme	sodium	213:218	arg1	sulfate					220:226	sodium sulfate	213:226	sodium sulfate (Na2SO4)	213:235	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	0	41	theme	functions	54:62	arg1	Comparison					0:9	Comparison	0:9	Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite	0:121	Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite microfibrillated cellulose composite.
28406056	1	42	theme	hydroxyapatite-modified	280:302	arg1	cellulose					321:329	calcium hydroxyapatite-modified microfibrillated cellulose	272:329	calcium hydroxyapatite-modified microfibrillated cellulose	272:329	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	2	43	dep	Fourier	426:432	arg1	transform					434:442	transform	434:442	transform infrared	434:451	The adsorbent was characterized using elemental analysis, Fourier transform infrared, scanning electron microscope and elemental analysis in order to gain the information on its structure and physico-chemical properties.
28406056	6	44	theme	7.35 mg g-1	1120:1130	arg1	sulfates					1100:1107	sulfates	1100:1107	sulfates of SLS and 7.35 mg g-1 for sulfates of Na2SO4	1100:1153	Experimental maximum adsorption capacities were found to be 34.53 mg g-1 for sulfates of SLS and 7.35 mg g-1 for sulfates of Na2SO4.
28406056	6	45	theme	Na2SO4	1148:1153	arg1	sulfates					1136:1143	sulfates	1136:1143	sulfates of Na2SO4	1136:1153	Experimental maximum adsorption capacities were found to be 34.53 mg g-1 for sulfates of SLS and 7.35 mg g-1 for sulfates of Na2SO4.
28406056	0	46	theme	error	48:52	arg1	functions					54:62	error functions	48:62	error functions	48:62	Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite microfibrillated cellulose composite.
28406056	1	47	theme	microfibrillated	304:319	arg1	cellulose					321:329	calcium hydroxyapatite-modified microfibrillated cellulose	272:329	calcium hydroxyapatite-modified microfibrillated cellulose	272:329	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	4	48	theme	sulfate	713:719	arg1	concentration					696:708	the initial concentration	684:708	the initial concentration of sulfate	684:719	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	4	48	theme	sulfate	713:719	arg1	effects					646:652	The effects	642:652	The effects of solution pH	642:667	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	4	48	theme	sulfate	713:719	arg1	time					678:681	contact time	670:681	contact time	670:681	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	4	48	theme	sulfate	713:719	arg1	effect					729:734	the effect	725:734	the effect of competing anions	725:754	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	1	49	theme	sulfate	220:226	arg1	sulfates					201:208	sulfates	201:208	sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS)	201:267	In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution.
28406056	5	50	theme	Adsorption	834:843	arg1	kinetics					845:852	Adsorption kinetics	834:852	Adsorption kinetics	834:852	Adsorption kinetics indicated very fast adsorption rate for sulfate of both sources (Na2SO4 and SLS) and the adsorption process was well described by the pseudo-second-order kinetic model.
28406056	7	51	theme	different	1280:1288	arg1	functions					1296:1304	five different error functions	1275:1304	five different error functions	1275:1304	The equilibrium data were described by the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions.
28406056	6	52	theme	maximum	1036:1042	arg1	capacities					1055:1064	Experimental maximum adsorption capacities	1023:1064	Experimental maximum adsorption capacities	1023:1064	Experimental maximum adsorption capacities were found to be 34.53 mg g-1 for sulfates of SLS and 7.35 mg g-1 for sulfates of Na2SO4.
28406056	7	53	theme	Toth	1227:1230	arg1	models					1262:1267	the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models	1195:1267	the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions	1195:1304	The equilibrium data were described by the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions.
28406056	2	54	theme	elemental	406:414	arg1	analysis					416:423	elemental analysis	406:423	elemental analysis	406:423	The adsorbent was characterized using elemental analysis, Fourier transform infrared, scanning electron microscope and elemental analysis in order to gain the information on its structure and physico-chemical properties.
28406056	3	55	theme	batch	630:634	arg1	mode					636:639	batch mode	630:639	batch mode	630:639	The adsorption studies were conducted in batch mode.
28406056	4	56	theme	initial	688:694	arg1	concentration					696:708	the initial concentration	684:708	the initial concentration of sulfate	684:719	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	6	57	theme	Experimental	1023:1034	arg1	capacities					1055:1064	Experimental maximum adsorption capacities	1023:1064	Experimental maximum adsorption capacities	1023:1064	Experimental maximum adsorption capacities were found to be 34.53 mg g-1 for sulfates of SLS and 7.35 mg g-1 for sulfates of Na2SO4.
28406056	4	58	theme	adsorbent	803:811	arg1	performance					776:786	the performance	772:786	the performance of synthesized adsorbent for sulfate removal	772:831	The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal.
28406056	6	59	theme	adsorption	1044:1053	arg1	capacities					1055:1064	Experimental maximum adsorption capacities	1023:1064	Experimental maximum adsorption capacities	1023:1064	Experimental maximum adsorption capacities were found to be 34.53 mg g-1 for sulfates of SLS and 7.35 mg g-1 for sulfates of Na2SO4.
28406056	7	60	theme	Redlich-Peterson	1236:1251	arg1	isotherm					1253:1260	Redlich-Peterson isotherm	1236:1260	Redlich-Peterson isotherm	1236:1260	The equilibrium data were described by the Langmuir, Sips, Freundlich, Toth and Redlich-Peterson isotherm models using five different error functions.
25265049	7	0	theme	·OH	1245:1247	arg1	radicals					1249:1256	significantly more ·OH radicals	1226:1256	significantly more ·OH radicals	1226:1256	In a cell-free reaction with H₂O₂, radicals were observed; the CNCs and a CNF generated significantly more ·OH radicals than the CMC (p < 0.05).
25265049	10	1	theme	potential	1652:1660	arg1	hazards					1673:1679	the potential inhalation hazards	1648:1679	the potential inhalation hazards of cellulose	1648:1692	The production of ·OH radicals indicates the need for additional studies to characterize the potential inhalation hazards of cellulose.
25265049	5	2	from	D	984:984	arg1	SUF					989:991	SUF	989:991	SUF	989:991	For one CNC, a slight decrease in x(CR) and D in SUF was observed.
25265049	5	2	from	D	984:984	arg1	CR					976:977	CR	976:977	CR	976:977	For one CNC, a slight decrease in x(CR) and D in SUF was observed.
25265049	5	2	from	D	984:984	arg1	x					974:974	x	974:974	x(CR)	974:978	For one CNC, a slight decrease in x(CR) and D in SUF was observed.
25265049	2	3	theme	cellulose	344:352	arg1	nanofibril					354:363	two cellulose nanofibril	340:363	two cellulose nanofibril (CNF)	340:369	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	3	theme	cellulose	344:352	arg1	CNF					366:368	CNF	366:368	CNF	366:368	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	8	4	theme	surface	1392:1398	arg1	area					1400:1403	the higher surface area	1381:1403	the higher surface area to volume ratio of the CNCs	1381:1431	The ·OH radical production correlates with particle decomposition temperature and is explained by the higher surface area to volume ratio of the CNCs.
25265049	3	5	theme	thermogravimetry	643:658	arg1	capacity					741:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity	643:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples	643:763	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	10	6	theme	cellulose	1684:1692	arg1	hazards					1673:1679	the potential inhalation hazards	1648:1679	the potential inhalation hazards of cellulose	1648:1692	The production of ·OH radicals indicates the need for additional studies to characterize the potential inhalation hazards of cellulose.
25265049	9	7	theme	mechanical	1464:1473	arg1	mechanism					1506:1514	the primary mechanism	1494:1514	the primary mechanism for lung clearance of cellulose materials	1494:1556	Based on their biodurability, mechanical clearance would be the primary mechanism for lung clearance of cellulose materials.
25265049	9	7	theme	mechanical	1464:1473	arg1	clearance					1475:1483	mechanical clearance	1464:1483	mechanical clearance	1464:1483	Based on their biodurability, mechanical clearance would be the primary mechanism for lung clearance of cellulose materials.
25265049	2	8	theme	nanocrystal	321:331	arg1	biodurability					288:300	the biodurability	284:300	the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC)	284:414	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	6	9	theme	exposure	1054:1061	arg1	time					1063:1066	exposure time	1054:1066	exposure time	1054:1066	In acidic PSF, a slight increase in x(CR) with exposure time was observed, possibly due to dissolution of the amorphous component.
25265049	3	10	theme	surface	661:667	arg1	area					669:672	surface area	661:672	surface area	661:672	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	7	11	theme	cell-free	1143:1151	arg1	reaction					1153:1160	a cell-free reaction	1141:1160	a cell-free reaction with H₂O₂	1141:1170	In a cell-free reaction with H₂O₂, radicals were observed; the CNCs and a CNF generated significantly more ·OH radicals than the CMC (p < 0.05).
25265049	3	12	theme	zeta	698:701	arg1	potential					703:711	zeta potential	698:711	zeta potential	698:711	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	1	13	theme	human	239:243	arg1	health					245:250	human health	239:250	human health	239:250	Abstract The potential applications of cellulose nanomaterials in advanced composites and biomedicine makes it imperative to understand their pulmonary exposure to human health.
25265049	2	14	theme	phagolysosomal	523:536	arg1	PSF					545:547	PSF	545:547	PSF	545:547	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	14	theme	phagolysosomal	523:536	arg1	fluid					538:542	alveolar macrophage phagolysosomal fluid	503:542	alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months	503:575	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	1	15	dep	Abstract	75:82	arg1	applications					98:109	The potential applications	84:109	The potential applications of cellulose nanomaterials in advanced composites	84:159	Abstract The potential applications of cellulose nanomaterials in advanced composites and biomedicine makes it imperative to understand their pulmonary exposure to human health.
25265049	3	16	theme	diameter	688:695	arg1	capacity					741:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity	643:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples	643:763	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	2	17	theme	alveolar	503:510	arg1	PSF					545:547	PSF	545:547	PSF	545:547	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	17	theme	alveolar	503:510	arg1	fluid					538:542	alveolar macrophage phagolysosomal fluid	503:542	alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months	503:575	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	3	18	theme	radical	722:728	arg1	generation					730:739	free radical generation	717:739	free radical generation	717:739	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	10	19	theme	·OH	1577:1579	arg1	radicals					1581:1588	·OH radicals	1577:1588	·OH radicals	1577:1588	The production of ·OH radicals indicates the need for additional studies to characterize the potential inhalation hazards of cellulose.
25265049	0	20	theme	nanomaterials	60:72	arg1	production					36:45	free radical production	23:45	free radical production	23:45	Lung biodurability and free radical production of cellulose nanomaterials.
25265049	0	20	theme	nanomaterials	60:72	arg1	biodurability					5:17	Lung biodurability	0:17	Lung biodurability	0:17	Lung biodurability and free radical production of cellulose nanomaterials.
25265049	9	21	theme	primary	1498:1504	arg1	mechanism					1506:1514	the primary mechanism	1494:1514	the primary mechanism for lung clearance of cellulose materials	1494:1556	Based on their biodurability, mechanical clearance would be the primary mechanism for lung clearance of cellulose materials.
25265049	9	21	theme	primary	1498:1504	arg1	clearance					1475:1483	mechanical clearance	1464:1483	mechanical clearance	1464:1483	Based on their biodurability, mechanical clearance would be the primary mechanism for lung clearance of cellulose materials.
25265049	8	22	dep	area	1400:1403	arg1	ratio					1415:1419	volume ratio	1408:1419	the higher surface area to volume ratio of the CNCs	1381:1431	The ·OH radical production correlates with particle decomposition temperature and is explained by the higher surface area to volume ratio of the CNCs.
25265049	8	22	dep	area	1400:1403	arg1	to					1405:1406	to	1405:1406	to	1405:1406	The ·OH radical production correlates with particle decomposition temperature and is explained by the higher surface area to volume ratio of the CNCs.
25265049	6	23	theme	component	1127:1135	arg1	dissolution					1098:1108	dissolution	1098:1108	dissolution of the amorphous component	1098:1135	In acidic PSF, a slight increase in x(CR) with exposure time was observed, possibly due to dissolution of the amorphous component.
25265049	3	24	theme	line	820:823	arg1	models					825:830	RAW 264.7 cell line models	805:830	RAW 264.7 cell line models	805:830	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	2	25	theme	benchmark	377:385	arg1	microcrystal					397:408	a benchmark cellulose microcrystal	375:408	a benchmark cellulose microcrystal (CMC)	375:414	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	25	theme	benchmark	377:385	arg1	CMC					411:413	CMC	411:413	CMC	411:413	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	7	26	with	reaction	1153:1160	arg1	H₂O₂					1167:1170	H₂O₂	1167:1170	H₂O₂	1167:1170	In a cell-free reaction with H₂O₂, radicals were observed; the CNCs and a CNF generated significantly more ·OH radicals than the CMC (p < 0.05).
25265049	9	27	theme	cellulose	1538:1546	arg1	materials					1548:1556	cellulose materials	1538:1556	cellulose materials	1538:1556	Based on their biodurability, mechanical clearance would be the primary mechanism for lung clearance of cellulose materials.
25265049	0	28	theme	Lung	0:3	arg1	biodurability					5:17	Lung biodurability	0:17	Lung biodurability	0:17	Lung biodurability and free radical production of cellulose nanomaterials.
25265049	1	29	theme	cellulose	114:122	arg1	nanomaterials					124:136	cellulose nanomaterials	114:136	cellulose nanomaterials	114:136	Abstract The potential applications of cellulose nanomaterials in advanced composites and biomedicine makes it imperative to understand their pulmonary exposure to human health.
25265049	2	30	from	results	273:279	arg1	biodurability					288:300	the biodurability	284:300	the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC)	284:414	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	31	theme	lining	455:460	arg1	SUF					469:471	SUF	469:471	SUF	469:471	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	31	theme	lining	455:460	arg1	fluid					462:466	artificial lung airway lining fluid	432:466	artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days	432:497	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	8	32	theme	radical	1291:1297	arg1	production					1299:1308	The ·OH radical production	1283:1308	The ·OH radical production	1283:1308	The ·OH radical production correlates with particle decomposition temperature and is explained by the higher surface area to volume ratio of the CNCs.
25265049	2	33	theme	microcrystal	397:408	arg1	biodurability					288:300	the biodurability	284:300	the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC)	284:414	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	0	34	theme	radical	28:34	arg1	production					36:45	free radical production	23:45	free radical production	23:45	Lung biodurability and free radical production of cellulose nanomaterials.
25265049	2	35	theme	lung	443:446	arg1	SUF					469:471	SUF	469:471	SUF	469:471	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	35	theme	lung	443:446	arg1	fluid					462:466	artificial lung airway lining fluid	432:466	artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days	432:497	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	7	36	located	observed	1187:1194	arg2	radicals					1173:1180	radicals	1173:1180	radicals	1173:1180	In a cell-free reaction with H₂O₂, radicals were observed; the CNCs and a CNF generated significantly more ·OH radicals than the CMC (p < 0.05).
25265049	7	36	located	observed	1187:1194	arg1	reaction					1153:1160	a cell-free reaction	1141:1160	a cell-free reaction with H₂O₂	1141:1170	In a cell-free reaction with H₂O₂, radicals were observed; the CNCs and a CNF generated significantly more ·OH radicals than the CMC (p < 0.05).
25265049	8	37	theme	particle	1326:1333	arg1	temperature					1349:1359	particle decomposition temperature	1326:1359	particle decomposition temperature	1326:1359	The ·OH radical production correlates with particle decomposition temperature and is explained by the higher surface area to volume ratio of the CNCs.
25265049	2	38	dep	SUF	469:471	arg1	pH					474:475	pH 7.3	474:479	pH 7.3	474:479	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	3	39	theme	X-ray	578:582	arg1	analysis					596:603	X-ray diffraction analysis	578:603	X-ray diffraction analysis	578:603	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	8	40	theme	volume	1408:1413	arg1	ratio					1415:1419	volume ratio	1408:1419	the higher surface area to volume ratio of the CNCs	1381:1431	The ·OH radical production correlates with particle decomposition temperature and is explained by the higher surface area to volume ratio of the CNCs.
25265049	0	41	theme	cellulose	50:58	arg1	nanomaterials					60:72	cellulose nanomaterials	50:72	cellulose nanomaterials	50:72	Lung biodurability and free radical production of cellulose nanomaterials.
25265049	7	42	theme	more	1240:1243	arg1	radicals					1249:1256	significantly more ·OH radicals	1226:1256	significantly more ·OH radicals	1226:1256	In a cell-free reaction with H₂O₂, radicals were observed; the CNCs and a CNF generated significantly more ·OH radicals than the CMC (p < 0.05).
25265049	10	43	theme	inhalation	1662:1671	arg1	hazards					1673:1679	the potential inhalation hazards	1648:1679	the potential inhalation hazards of cellulose	1648:1692	The production of ·OH radicals indicates the need for additional studies to characterize the potential inhalation hazards of cellulose.
25265049	2	44	theme	nanofibril	354:363	arg1	biodurability					288:300	the biodurability	284:300	the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC)	284:414	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	4	45	from	changes	863:869	arg1	SUF					928:930	SUF	928:930	SUF	928:930	The CMC showed no measurable changes in crystallinity (x(CR)) or crystallite size D in either SUF or PSF.
25265049	4	45	from	changes	863:869	arg1	crystallinity					874:886	crystallinity	874:886	crystallinity (x(CR))	874:894	The CMC showed no measurable changes in crystallinity (x(CR)) or crystallite size D in either SUF or PSF.
25265049	4	45	from	changes	863:869	arg1	PSF					935:937	PSF	935:937	PSF	935:937	The CMC showed no measurable changes in crystallinity (x(CR)) or crystallite size D in either SUF or PSF.
25265049	4	45	from	changes	863:869	arg1	D					916:916	crystallite size D	899:916	crystallite size D	899:916	The CMC showed no measurable changes in crystallinity (x(CR)) or crystallite size D in either SUF or PSF.
25265049	8	46	theme	CNCs	1428:1431	arg1	area					1400:1403	the higher surface area	1381:1403	the higher surface area to volume ratio of the CNCs	1381:1431	The ·OH radical production correlates with particle decomposition temperature and is explained by the higher surface area to volume ratio of the CNCs.
25265049	8	47	theme	higher	1385:1390	arg1	area					1400:1403	the higher surface area	1381:1403	the higher surface area to volume ratio of the CNCs	1381:1431	The ·OH radical production correlates with particle decomposition temperature and is explained by the higher surface area to volume ratio of the CNCs.
25265049	2	48	dep	7	492:492	arg1	to					489:490	to	489:490	to	489:490	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	3	49	theme	hydrodynamic	675:686	arg1	diameter					688:695	hydrodynamic diameter	675:695	hydrodynamic diameter	675:695	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	2	50	theme	cellulose	311:319	arg1	CNC					334:336	CNC	334:336	CNC	334:336	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	50	theme	cellulose	311:319	arg1	nanocrystal					321:331	three cellulose nanocrystal	305:331	three cellulose nanocrystal (CNC)	305:337	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	1	51	theme	pulmonary	217:225	arg1	exposure					227:234	their pulmonary exposure	211:234	their pulmonary exposure to human health	211:250	Abstract The potential applications of cellulose nanomaterials in advanced composites and biomedicine makes it imperative to understand their pulmonary exposure to human health.
25265049	3	52	theme	area	669:672	arg1	capacity					741:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity	643:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples	643:763	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	6	53	theme	slight	1024:1029	arg1	increase					1031:1038	a slight increase	1022:1038	a slight increase in x(CR) with exposure time	1022:1066	In acidic PSF, a slight increase in x(CR) with exposure time was observed, possibly due to dissolution of the amorphous component.
25265049	3	54	theme	potential	703:711	arg1	capacity					741:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity	643:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples	643:763	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	1	55	theme	nanomaterials	124:136	arg1	applications					98:109	The potential applications	84:109	The potential applications of cellulose nanomaterials in advanced composites	84:159	Abstract The potential applications of cellulose nanomaterials in advanced composites and biomedicine makes it imperative to understand their pulmonary exposure to human health.
25265049	2	56	dep	PSF	545:547	arg1	pH					550:551	pH 4.5	550:555	pH 4.5	550:555	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	57	theme	macrophage	512:521	arg1	PSF					545:547	PSF	545:547	PSF	545:547	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	57	theme	macrophage	512:521	arg1	fluid					538:542	alveolar macrophage phagolysosomal fluid	503:542	alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months	503:575	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	4	58	dep	crystallinity	874:886	arg1	CR					891:892	CR	891:892	CR	891:892	The CMC showed no measurable changes in crystallinity (x(CR)) or crystallite size D in either SUF or PSF.
25265049	4	58	dep	crystallinity	874:886	arg1	x					889:889	x	889:889	x(CR)	889:893	The CMC showed no measurable changes in crystallinity (x(CR)) or crystallite size D in either SUF or PSF.
25265049	10	59	theme	radicals	1581:1588	arg1	production					1563:1572	The production	1559:1572	The production of ·OH radicals	1559:1588	The production of ·OH radicals indicates the need for additional studies to characterize the potential inhalation hazards of cellulose.
25265049	1	60	theme	advanced	141:148	arg1	composites					150:159	advanced composites	141:159	advanced composites	141:159	Abstract The potential applications of cellulose nanomaterials in advanced composites and biomedicine makes it imperative to understand their pulmonary exposure to human health.
25265049	6	61	theme	acidic	1010:1015	arg1	PSF					1017:1019	acidic PSF	1010:1019	acidic PSF	1010:1019	In acidic PSF, a slight increase in x(CR) with exposure time was observed, possibly due to dissolution of the amorphous component.
25265049	6	62	from	increase	1031:1038	arg1	CR					1045:1046	CR	1045:1046	CR	1045:1046	In acidic PSF, a slight increase in x(CR) with exposure time was observed, possibly due to dissolution of the amorphous component.
25265049	6	62	from	increase	1031:1038	arg1	x					1043:1043	x	1043:1043	x(CR) with exposure time	1043:1066	In acidic PSF, a slight increase in x(CR) with exposure time was observed, possibly due to dissolution of the amorphous component.
25265049	3	63	theme	generation	730:739	arg1	capacity					741:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity	643:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples	643:763	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	6	64	located	observed	1072:1079	arg2	increase					1031:1038	a slight increase	1022:1038	a slight increase in x(CR) with exposure time	1022:1066	In acidic PSF, a slight increase in x(CR) with exposure time was observed, possibly due to dissolution of the amorphous component.
25265049	6	64	located	observed	1072:1079	arg1	PSF					1017:1019	acidic PSF	1010:1019	acidic PSF	1010:1019	In acidic PSF, a slight increase in x(CR) with exposure time was observed, possibly due to dissolution of the amorphous component.
25265049	3	65	theme	free	717:720	arg1	generation					730:739	free radical generation	717:739	free radical generation	717:739	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	3	66	theme	RAW	805:807	arg1	models					825:830	RAW 264.7 cell line models	805:830	RAW 264.7 cell line models	805:830	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	3	67	theme	samples	757:763	arg1	capacity					741:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity	643:748	thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples	643:763	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	3	68	dep	determined	770:779	arg1	in					782:783	in	782:783	in	782:783	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	3	68	dep	determined	770:779	arg1	models					825:830	RAW 264.7 cell line models	805:830	RAW 264.7 cell line models	805:830	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	2	69	dep	9	568:568	arg1	to					565:566	to	565:566	to	565:566	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	9	70	theme	lung	1520:1523	arg1	clearance					1525:1533	lung clearance	1520:1533	lung clearance of cellulose materials	1520:1556	Based on their biodurability, mechanical clearance would be the primary mechanism for lung clearance of cellulose materials.
25265049	6	71	theme	amorphous	1117:1125	arg1	component					1127:1135	the amorphous component	1113:1135	the amorphous component	1113:1135	In acidic PSF, a slight increase in x(CR) with exposure time was observed, possibly due to dissolution of the amorphous component.
25265049	3	72	dep	in	782:783	arg1	cell-free					791:799	cell-free	791:799	cell-free	791:799	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	3	72	dep	in	782:783	arg1	vitro					785:789	vitro	785:789	vitro	785:789	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	4	73	theme	measurable	852:861	arg1	changes					863:869	no measurable changes	849:869	no measurable changes in crystallinity (x(CR)) or crystallite size D in either SUF or PSF	849:937	The CMC showed no measurable changes in crystallinity (x(CR)) or crystallite size D in either SUF or PSF.
25265049	5	74	theme	slight	955:960	arg1	decrease					962:969	a slight decrease	953:969	a slight decrease in x(CR)	953:978	For one CNC, a slight decrease in x(CR) and D in SUF was observed.
25265049	1	75	theme	potential	88:96	arg1	applications					98:109	The potential applications	84:109	The potential applications of cellulose nanomaterials in advanced composites	84:159	Abstract The potential applications of cellulose nanomaterials in advanced composites and biomedicine makes it imperative to understand their pulmonary exposure to human health.
25265049	3	76	theme	cell	815:818	arg1	models					825:830	RAW 264.7 cell line models	805:830	RAW 264.7 cell line models	805:830	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	9	77	theme	materials	1548:1556	arg1	clearance					1525:1533	lung clearance	1520:1533	lung clearance of cellulose materials	1520:1556	Based on their biodurability, mechanical clearance would be the primary mechanism for lung clearance of cellulose materials.
25265049	8	78	theme	·OH	1287:1289	arg1	production					1299:1308	The ·OH radical production	1283:1308	The ·OH radical production	1283:1308	The ·OH radical production correlates with particle decomposition temperature and is explained by the higher surface area to volume ratio of the CNCs.
25265049	10	79	theme	additional	1613:1622	arg1	studies					1624:1630	additional studies	1613:1630	additional studies	1613:1630	The production of ·OH radicals indicates the need for additional studies to characterize the potential inhalation hazards of cellulose.
25265049	0	80	theme	free	23:26	arg1	production					36:45	free radical production	23:45	free radical production	23:45	Lung biodurability and free radical production of cellulose nanomaterials.
25265049	4	81	theme	size	911:914	arg1	D					916:916	crystallite size D	899:916	crystallite size D	899:916	The CMC showed no measurable changes in crystallinity (x(CR)) or crystallite size D in either SUF or PSF.
25265049	1	82	from	applications	98:109	arg1	composites					150:159	advanced composites	141:159	advanced composites	141:159	Abstract The potential applications of cellulose nanomaterials in advanced composites and biomedicine makes it imperative to understand their pulmonary exposure to human health.
25265049	2	83	theme	airway	448:453	arg1	SUF					469:471	SUF	469:471	SUF	469:471	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	83	theme	airway	448:453	arg1	fluid					462:466	artificial lung airway lining fluid	432:466	artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days	432:497	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	84	theme	cellulose	387:395	arg1	microcrystal					397:408	a benchmark cellulose microcrystal	375:408	a benchmark cellulose microcrystal (CMC)	375:414	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	84	theme	cellulose	387:395	arg1	CMC					411:413	CMC	411:413	CMC	411:413	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	5	85	from	decrease	962:969	arg1	SUF					989:991	SUF	989:991	SUF	989:991	For one CNC, a slight decrease in x(CR) and D in SUF was observed.
25265049	5	85	from	decrease	962:969	arg1	CR					976:977	CR	976:977	CR	976:977	For one CNC, a slight decrease in x(CR) and D in SUF was observed.
25265049	5	85	from	decrease	962:969	arg1	x					974:974	x	974:974	x(CR)	974:978	For one CNC, a slight decrease in x(CR) and D in SUF was observed.
25265049	2	86	theme	artificial	432:441	arg1	SUF					469:471	SUF	469:471	SUF	469:471	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	2	86	theme	artificial	432:441	arg1	fluid					462:466	artificial lung airway lining fluid	432:466	artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days	432:497	Here, we report the results on the biodurability of three cellulose nanocrystal (CNC), two cellulose nanofibril (CNF) and a benchmark cellulose microcrystal (CMC) when exposed to artificial lung airway lining fluid (SUF, pH 7.3) for up to 7 days and alveolar macrophage phagolysosomal fluid (PSF, pH 4.5) for up to 9 months.
25265049	8	87	theme	decomposition	1335:1347	arg1	temperature					1349:1359	particle decomposition temperature	1326:1359	particle decomposition temperature	1326:1359	The ·OH radical production correlates with particle decomposition temperature and is explained by the higher surface area to volume ratio of the CNCs.
25265049	3	88	theme	diffraction	584:594	arg1	analysis					596:603	X-ray diffraction analysis	578:603	X-ray diffraction analysis	578:603	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	3	89	used	used	609:612	arg2	analysis					596:603	X-ray diffraction analysis	578:603	X-ray diffraction analysis	578:603	X-ray diffraction analysis was used to monitor biodurability and thermogravimetry, surface area, hydrodynamic diameter, zeta potential and free radical generation capacity of the samples were determined (in vitro cell-free and RAW 264.7 cell line models).
25265049	4	90	theme	crystallite	899:909	arg1	D					916:916	crystallite size D	899:916	crystallite size D	899:916	The CMC showed no measurable changes in crystallinity (x(CR)) or crystallite size D in either SUF or PSF.
25265049	6	91	with	x	1043:1043	arg1	time					1063:1066	exposure time	1054:1066	exposure time	1054:1066	In acidic PSF, a slight increase in x(CR) with exposure time was observed, possibly due to dissolution of the amorphous component.
24738363	8	0	theme	Swelling	1142:1149	arg1	studies					1151:1157	Swelling studies	1142:1157	Swelling studies	1142:1157	Swelling studies showed that the CH-Ag-E exhibits efficient water absorption property compared to that of CH-Ag-I.
24738363	6	1	theme	chitosan-Ag-ex-situ	904:922	arg1	gel					948:950	chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel	904:950	chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel	904:950	And, in second approach already prepared chitosan hydrogel was placed in Ag NPs solutions, resulting in adsorption of Ag NPs and thus forming chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel.
24738363	1	2	from	release	205:211	arg1	gel					182:184	chitosan gel	173:184	chitosan gel	173:184	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	7	3	theme	electron	1115:1122	arg1	SEM					1136:1138	SEM	1136:1138	SEM	1136:1138	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	7	3	theme	electron	1115:1122	arg1	microscopy					1124:1133	Scanning electron microscopy	1106:1133	Scanning electron microscopy (SEM)	1106:1139	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	2	4	theme	small	282:286	arg1	nanoparticles					300:312	small sized metal nanoparticles	282:312	small sized metal nanoparticles	282:312	The generation of small sized metal nanoparticles which acts as catalyst is prerequisite to progress of a catalytic reaction.
24738363	6	5	theme	NPs	883:885	arg1	adsorption					866:875	adsorption	866:875	adsorption of Ag NPs	866:885	And, in second approach already prepared chitosan hydrogel was placed in Ag NPs solutions, resulting in adsorption of Ag NPs and thus forming chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel.
24738363	5	6	theme	nanoparticles	621:633	arg1	incorporation					601:613	in-situ incorporation	593:613	in-situ incorporation of Ag nanoparticles into the reaction mixture	593:659	The first approach involves in-situ incorporation of Ag nanoparticles into the reaction mixture while preparing the chitosan hydrogel and termed as chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel.
24738363	6	7	theme	prepared	794:801	arg1	hydrogel					812:819	already prepared chitosan hydrogel	786:819	already prepared chitosan hydrogel	786:819	And, in second approach already prepared chitosan hydrogel was placed in Ag NPs solutions, resulting in adsorption of Ag NPs and thus forming chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel.
24738363	8	8	theme	water	1202:1206	arg1	property					1219:1226	efficient water absorption property	1192:1226	efficient water absorption property	1192:1226	Swelling studies showed that the CH-Ag-E exhibits efficient water absorption property compared to that of CH-Ag-I.
24738363	2	9	theme	metal	294:298	arg1	nanoparticles					300:312	small sized metal nanoparticles	282:312	small sized metal nanoparticles	282:312	The generation of small sized metal nanoparticles which acts as catalyst is prerequisite to progress of a catalytic reaction.
24738363	11	10	theme	Ag	1811:1812	arg1	NPs					1814:1816	the Ag NPs	1807:1816	the Ag NPs loaded in the gel (CH-Ag-E)	1807:1844	The reduction reaction catalyzed by such Ag NPs follow zero order kinetics and dependent on the size of the Ag NPs loaded in the gel (CH-Ag-E) as well as on the amount of the gel used.
24738363	7	11	theme	Fourier	1018:1024	arg1	FTIR					1062:1065	FTIR	1062:1065	FTIR	1062:1065	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	7	11	theme	Fourier	1018:1024	arg1	spectroscopy					1048:1059	Fourier transformed infra-red spectroscopy	1018:1059	Fourier transformed infra-red spectroscopy (FTIR)	1018:1066	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	0	12	dep	composite	49:57	arg1	comparison					60:69	comparison	60:69	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.	0:114	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.
24738363	6	13	theme	nanocomposite	934:946	arg1	gel					948:950	chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel	904:950	chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel	904:950	And, in second approach already prepared chitosan hydrogel was placed in Ag NPs solutions, resulting in adsorption of Ag NPs and thus forming chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel.
24738363	11	14	theme	Ag	1744:1745	arg1	NPs					1747:1749	such Ag NPs	1739:1749	such Ag NPs	1739:1749	The reduction reaction catalyzed by such Ag NPs follow zero order kinetics and dependent on the size of the Ag NPs loaded in the gel (CH-Ag-E) as well as on the amount of the gel used.
24738363	5	15	theme	chitosan	681:688	arg1	hydrogel					690:697	the chitosan hydrogel	677:697	the chitosan hydrogel	677:697	The first approach involves in-situ incorporation of Ag nanoparticles into the reaction mixture while preparing the chitosan hydrogel and termed as chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel.
24738363	12	16	from	NPs	1939:1941	arg1	CH-Ag-E					1946:1952	CH-Ag-E	1946:1952	CH-Ag-E	1946:1952	We found that smaller is the size of the loaded Ag NPs in CH-Ag-E, more effective it is in catalyzing the reduction reaction.
24738363	12	17	theme	Ag	1936:1937	arg1	NPs					1939:1941	the loaded Ag NPs	1925:1941	the loaded Ag NPs in CH-Ag-E	1925:1952	We found that smaller is the size of the loaded Ag NPs in CH-Ag-E, more effective it is in catalyzing the reduction reaction.
24738363	11	18	theme	gel	1878:1880	arg1	gel					1878:1880	the gel	1874:1880	the gel used	1874:1885	The reduction reaction catalyzed by such Ag NPs follow zero order kinetics and dependent on the size of the Ag NPs loaded in the gel (CH-Ag-E) as well as on the amount of the gel used.
24738363	11	18	theme	gel	1878:1880	arg1	amount					1864:1869	the amount	1860:1869	the amount of the gel used	1860:1885	The reduction reaction catalyzed by such Ag NPs follow zero order kinetics and dependent on the size of the Ag NPs loaded in the gel (CH-Ag-E) as well as on the amount of the gel used.
24738363	12	19	from	size	1917:1920	arg1	CH-Ag-E					1946:1952	CH-Ag-E	1946:1952	CH-Ag-E	1946:1952	We found that smaller is the size of the loaded Ag NPs in CH-Ag-E, more effective it is in catalyzing the reduction reaction.
24738363	1	20	theme	silver	140:145	arg1	nanoparticles					147:159	silver nanoparticles	140:159	silver nanoparticles (Ag NPs) in chitosan gel	140:184	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	1	20	theme	silver	140:145	arg1	NPs					165:167	Ag NPs	162:167	Ag NPs	162:167	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	1	21	from	gel	182:184	arg1	storage					129:135	storage	129:135	storage of silver nanoparticles (Ag NPs) in chitosan gel	129:184	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	1	21	from	gel	182:184	arg1	release					205:211	its subsequent release	190:211	its subsequent release for catalytic reduction processes	190:245	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	9	22	theme	swelling	1282:1289	arg1	properties					1291:1300	efficient swelling properties	1272:1300	efficient swelling properties	1272:1300	In addition to efficient swelling properties the CH-Ag-E can also act as store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I.
24738363	7	23	theme	prepared	957:964	arg1	gels					966:969	The prepared gels	953:969	The prepared gels	953:969	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	0	24	theme	properties	104:113	arg1	comparison					60:69	comparison	60:69	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.	0:114	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.
24738363	1	25	from	nanoparticles	147:159	arg1	gel					182:184	chitosan gel	173:184	chitosan gel	173:184	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	3	26	theme	chitosan	425:432	arg1	gel					434:436	chitosan gel	425:436	chitosan gel	425:436	We show that Ag NPs extracted from chitosan gel are less than 5 nm so very effective in catalysis.
24738363	3	27	dep	effective	465:473	arg1	nm					454:455	less than 5 nm	442:455	less than 5 nm	442:455	We show that Ag NPs extracted from chitosan gel are less than 5 nm so very effective in catalysis.
24738363	3	27	dep	effective	465:473	arg1	NPs					406:408	Ag NPs	403:408	Ag NPs extracted from chitosan gel	403:436	We show that Ag NPs extracted from chitosan gel are less than 5 nm so very effective in catalysis.
24738363	2	28	theme	reaction	380:387	arg1	progress					356:363	progress	356:363	progress of a catalytic reaction	356:387	The generation of small sized metal nanoparticles which acts as catalyst is prerequisite to progress of a catalytic reaction.
24738363	0	29	theme	In-situ	0:6	arg1	composite					49:57	In-situ and ex-situ chitosan-silver nanoparticle composite	0:57	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.	0:114	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.
24738363	9	30	theme	store	1330:1334	arg1	house					1336:1340	store house	1330:1340	store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I	1330:1575	In addition to efficient swelling properties the CH-Ag-E can also act as store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I.
24738363	5	31	theme	first	569:573	arg1	approach					575:582	The first approach	565:582	The first approach	565:582	The first approach involves in-situ incorporation of Ag nanoparticles into the reaction mixture while preparing the chitosan hydrogel and termed as chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel.
24738363	9	32	theme	sodium	1517:1522	arg1	borohydride					1524:1534	sodium borohydride	1517:1534	sodium borohydride which is not possible in case of CH-Ag-I	1517:1575	In addition to efficient swelling properties the CH-Ag-E can also act as store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I.
24738363	0	33	theme	ex-situ	12:18	arg1	composite					49:57	In-situ and ex-situ chitosan-silver nanoparticle composite	0:57	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.	0:114	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.
24738363	1	34	theme	nanoparticles	147:159	arg1	storage					129:135	storage	129:135	storage of silver nanoparticles (Ag NPs) in chitosan gel	129:184	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	1	34	theme	nanoparticles	147:159	arg1	release					205:211	its subsequent release	190:211	its subsequent release for catalytic reduction processes	190:245	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	3	35	theme	Ag	403:404	arg1	nm					454:455	less than 5 nm	442:455	less than 5 nm	442:455	We show that Ag NPs extracted from chitosan gel are less than 5 nm so very effective in catalysis.
24738363	3	35	theme	Ag	403:404	arg1	NPs					406:408	Ag NPs	403:408	Ag NPs extracted from chitosan gel	403:436	We show that Ag NPs extracted from chitosan gel are less than 5 nm so very effective in catalysis.
24738363	4	36	theme	different	543:551	arg1	approaches					553:562	two different approaches	539:562	two different approaches	539:562	Chitosan-Ag nanocomposite gels were prepared from two different approaches.
24738363	0	37	theme	nanoparticle	36:47	arg1	composite					49:57	In-situ and ex-situ chitosan-silver nanoparticle composite	0:57	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.	0:114	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.
24738363	9	38	theme	NPs	1348:1350	arg1	house					1336:1340	store house	1330:1340	store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I	1330:1575	In addition to efficient swelling properties the CH-Ag-E can also act as store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I.
24738363	4	39	theme	nanocomposite	501:513	arg1	gels					515:518	Chitosan-Ag nanocomposite gels	489:518	Chitosan-Ag nanocomposite gels	489:518	Chitosan-Ag nanocomposite gels were prepared from two different approaches.
24738363	10	40	theme	nanocomposite	1639:1651	arg1	catalyst					1668:1675	catalyst	1668:1675	catalyst in comparison to CH-Ag-I	1668:1700	The rate of the reaction increases upto 10 fold when CH-Ag-E nanocomposite gel is used as catalyst in comparison to CH-Ag-I.
24738363	10	40	theme	nanocomposite	1639:1651	arg1	gel					1653:1655	CH-Ag-E nanocomposite gel	1631:1655	CH-Ag-E nanocomposite gel	1631:1655	The rate of the reaction increases upto 10 fold when CH-Ag-E nanocomposite gel is used as catalyst in comparison to CH-Ag-I.
24738363	11	41	theme	order	1763:1767	arg1	kinetics					1769:1776	zero order kinetics	1758:1776	zero order kinetics	1758:1776	The reduction reaction catalyzed by such Ag NPs follow zero order kinetics and dependent on the size of the Ag NPs loaded in the gel (CH-Ag-E) as well as on the amount of the gel used.
24738363	6	42	theme	Ag	835:836	arg1	solutions					842:850	Ag NPs solutions	835:850	Ag NPs solutions	835:850	And, in second approach already prepared chitosan hydrogel was placed in Ag NPs solutions, resulting in adsorption of Ag NPs and thus forming chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel.
24738363	12	43	theme	reduction	1994:2002	arg1	reaction					2004:2011	the reduction reaction	1990:2011	the reduction reaction	1990:2011	We found that smaller is the size of the loaded Ag NPs in CH-Ag-E, more effective it is in catalyzing the reduction reaction.
24738363	1	44	theme	reduction	227:235	arg1	processes					237:245	catalytic reduction processes	217:245	catalytic reduction processes	217:245	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	5	45	theme	reaction	644:651	arg1	mixture					653:659	the reaction mixture	640:659	the reaction mixture	640:659	The first approach involves in-situ incorporation of Ag nanoparticles into the reaction mixture while preparing the chitosan hydrogel and termed as chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel.
24738363	7	46	theme	Scanning	1106:1113	arg1	SEM					1136:1138	SEM	1136:1138	SEM	1136:1138	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	7	46	theme	Scanning	1106:1113	arg1	microscopy					1124:1133	Scanning electron microscopy	1106:1133	Scanning electron microscopy (SEM)	1106:1139	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	3	47	from	catalysis	478:486	arg1	effective					465:473	effective	465:473	effective	465:473	We show that Ag NPs extracted from chitosan gel are less than 5 nm so very effective in catalysis.
24738363	2	48	theme	sized	288:292	arg1	nanoparticles					300:312	small sized metal nanoparticles	282:312	small sized metal nanoparticles	282:312	The generation of small sized metal nanoparticles which acts as catalyst is prerequisite to progress of a catalytic reaction.
24738363	9	49	theme	4-Nitrophenol	1398:1410	arg1	reduction					1385:1393	the reduction	1381:1393	the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP)	1381:1441	In addition to efficient swelling properties the CH-Ag-E can also act as store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I.
24738363	5	50	theme	Ag	618:619	arg1	nanoparticles					621:633	Ag nanoparticles	618:633	Ag nanoparticles	618:633	The first approach involves in-situ incorporation of Ag nanoparticles into the reaction mixture while preparing the chitosan hydrogel and termed as chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel.
24738363	6	51	theme	chitosan	803:810	arg1	hydrogel					812:819	already prepared chitosan hydrogel	786:819	already prepared chitosan hydrogel	786:819	And, in second approach already prepared chitosan hydrogel was placed in Ag NPs solutions, resulting in adsorption of Ag NPs and thus forming chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel.
24738363	9	52	used	used	1364:1367	arg2	NPs					1348:1350	Ag NPs	1345:1350	Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I	1345:1575	In addition to efficient swelling properties the CH-Ag-E can also act as store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I.
24738363	6	53	theme	Ag	880:881	arg1	NPs					883:885	Ag NPs	880:885	Ag NPs	880:885	And, in second approach already prepared chitosan hydrogel was placed in Ag NPs solutions, resulting in adsorption of Ag NPs and thus forming chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel.
24738363	1	54	theme	chitosan	173:180	arg1	gel					182:184	chitosan gel	173:184	chitosan gel	173:184	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	11	55	theme	such	1739:1742	arg1	NPs					1747:1749	such Ag NPs	1739:1749	such Ag NPs	1739:1749	The reduction reaction catalyzed by such Ag NPs follow zero order kinetics and dependent on the size of the Ag NPs loaded in the gel (CH-Ag-E) as well as on the amount of the gel used.
24738363	7	56	theme	Thermogravimetric	1069:1085	arg1	TGA					1097:1099	TGA	1097:1099	TGA	1097:1099	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	7	56	theme	Thermogravimetric	1069:1085	arg1	analysis					1087:1094	Thermogravimetric analysis	1069:1094	Thermogravimetric analysis (TGA)	1069:1100	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	6	57	theme	second	770:775	arg1	approach					777:784	second approach	770:784	second approach	770:784	And, in second approach already prepared chitosan hydrogel was placed in Ag NPs solutions, resulting in adsorption of Ag NPs and thus forming chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel.
24738363	2	58	theme	nanoparticles	300:312	arg1	generation					268:277	The generation	264:277	The generation of small sized metal nanoparticles which acts as catalyst	264:335	The generation of small sized metal nanoparticles which acts as catalyst is prerequisite to progress of a catalytic reaction.
24738363	2	58	theme	nanoparticles	300:312	arg1	prerequisite					340:351	prerequisite	340:351	prerequisite to progress of a catalytic reaction	340:387	The generation of small sized metal nanoparticles which acts as catalyst is prerequisite to progress of a catalytic reaction.
24738363	8	59	theme	efficient	1192:1200	arg1	property					1219:1226	efficient water absorption property	1192:1226	efficient water absorption property	1192:1226	Swelling studies showed that the CH-Ag-E exhibits efficient water absorption property compared to that of CH-Ag-I.
24738363	12	60	theme	NPs	1939:1941	arg1	smaller					1902:1908	smaller	1902:1908	smaller	1902:1908	We found that smaller is the size of the loaded Ag NPs in CH-Ag-E, more effective it is in catalyzing the reduction reaction.
24738363	12	60	theme	NPs	1939:1941	arg1	size					1917:1920	the size	1913:1920	the size of the loaded Ag NPs in CH-Ag-E	1913:1952	We found that smaller is the size of the loaded Ag NPs in CH-Ag-E, more effective it is in catalyzing the reduction reaction.
24738363	5	61	theme	chitosan-Ag-in-situ	713:731	arg1	gel					757:759	chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel	713:759	chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel	713:759	The first approach involves in-situ incorporation of Ag nanoparticles into the reaction mixture while preparing the chitosan hydrogel and termed as chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel.
24738363	1	62	theme	subsequent	194:203	arg1	release					205:211	its subsequent release	190:211	its subsequent release for catalytic reduction processes	190:245	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	8	63	theme	absorption	1208:1217	arg1	property					1219:1226	efficient water absorption property	1192:1226	efficient water absorption property	1192:1226	Swelling studies showed that the CH-Ag-E exhibits efficient water absorption property compared to that of CH-Ag-I.
24738363	7	64	theme	transformed	1026:1036	arg1	FTIR					1062:1065	FTIR	1062:1065	FTIR	1062:1065	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	7	64	theme	transformed	1026:1036	arg1	spectroscopy					1048:1059	Fourier transformed infra-red spectroscopy	1018:1059	Fourier transformed infra-red spectroscopy (FTIR)	1018:1066	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	9	65	theme	Ag	1446:1447	arg1	NPs					1449:1451	Ag NPs	1446:1451	Ag NPs of this composite	1446:1469	In addition to efficient swelling properties the CH-Ag-E can also act as store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I.
24738363	3	66	from	effective	465:473	arg1	catalysis					478:486	catalysis	478:486	catalysis	478:486	We show that Ag NPs extracted from chitosan gel are less than 5 nm so very effective in catalysis.
24738363	7	67	theme	infra-red	1038:1046	arg1	FTIR					1062:1065	FTIR	1062:1065	FTIR	1062:1065	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	7	67	theme	infra-red	1038:1046	arg1	spectroscopy					1048:1059	Fourier transformed infra-red spectroscopy	1018:1059	Fourier transformed infra-red spectroscopy (FTIR)	1018:1066	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	10	68	from	catalyst	1668:1675	arg1	comparison					1680:1689	comparison	1680:1689	comparison to CH-Ag-I	1680:1700	The rate of the reaction increases upto 10 fold when CH-Ag-E nanocomposite gel is used as catalyst in comparison to CH-Ag-I.
24738363	12	69	theme	loaded	1929:1934	arg1	NPs					1939:1941	the loaded Ag NPs	1925:1941	the loaded Ag NPs in CH-Ag-E	1925:1952	We found that smaller is the size of the loaded Ag NPs in CH-Ag-E, more effective it is in catalyzing the reduction reaction.
24738363	0	70	theme	storage/release	74:88	arg1	comparison					60:69	comparison	60:69	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.	0:114	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.
24738363	9	71	theme	efficient	1272:1280	arg1	properties					1291:1300	efficient swelling properties	1272:1300	efficient swelling properties	1272:1300	In addition to efficient swelling properties the CH-Ag-E can also act as store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I.
24738363	7	72	theme	UV-Visible	993:1002	arg1	spectroscopy					1004:1015	UV-Visible spectroscopy	993:1015	UV-Visible spectroscopy	993:1015	The prepared gels were characterized by UV-Visible spectroscopy, Fourier transformed infra-red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and Scanning electron microscopy (SEM).
24738363	9	73	theme	composite	1461:1469	arg1	NPs					1449:1451	Ag NPs	1446:1451	Ag NPs of this composite	1446:1469	In addition to efficient swelling properties the CH-Ag-E can also act as store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I.
24738363	0	74	theme	catalytic	94:102	arg1	properties					104:113	catalytic properties	94:113	catalytic properties	94:113	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.
24738363	1	75	theme	Ag	162:163	arg1	nanoparticles					147:159	silver nanoparticles	140:159	silver nanoparticles (Ag NPs) in chitosan gel	140:184	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	1	75	theme	Ag	162:163	arg1	NPs					165:167	Ag NPs	162:167	Ag NPs	162:167	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	4	76	theme	Chitosan-Ag	489:499	arg1	gels					515:518	Chitosan-Ag nanocomposite gels	489:518	Chitosan-Ag nanocomposite gels	489:518	Chitosan-Ag nanocomposite gels were prepared from two different approaches.
24738363	11	77	theme	reduction	1707:1715	arg1	reaction					1717:1724	The reduction reaction	1703:1724	The reduction reaction catalyzed by such Ag NPs	1703:1749	The reduction reaction catalyzed by such Ag NPs follow zero order kinetics and dependent on the size of the Ag NPs loaded in the gel (CH-Ag-E) as well as on the amount of the gel used.
24738363	5	78	theme	CH-Ag-I	734:740	arg1	gel					757:759	chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel	713:759	chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel	713:759	The first approach involves in-situ incorporation of Ag nanoparticles into the reaction mixture while preparing the chitosan hydrogel and termed as chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel.
24738363	5	79	theme	in-situ	593:599	arg1	incorporation					601:613	in-situ incorporation	593:613	in-situ incorporation of Ag nanoparticles into the reaction mixture	593:659	The first approach involves in-situ incorporation of Ag nanoparticles into the reaction mixture while preparing the chitosan hydrogel and termed as chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel.
24738363	5	80	theme	nanocomposite	743:755	arg1	gel					757:759	chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel	713:759	chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel	713:759	The first approach involves in-situ incorporation of Ag nanoparticles into the reaction mixture while preparing the chitosan hydrogel and termed as chitosan-Ag-in-situ (CH-Ag-I) nanocomposite gel.
24738363	12	81	from	CH-Ag-E	1946:1952	arg1	smaller					1902:1908	smaller	1902:1908	smaller	1902:1908	We found that smaller is the size of the loaded Ag NPs in CH-Ag-E, more effective it is in catalyzing the reduction reaction.
24738363	12	81	from	CH-Ag-E	1946:1952	arg1	size					1917:1920	the size	1913:1920	the size of the loaded Ag NPs in CH-Ag-E	1913:1952	We found that smaller is the size of the loaded Ag NPs in CH-Ag-E, more effective it is in catalyzing the reduction reaction.
24738363	2	82	theme	catalytic	370:378	arg1	reaction					380:387	a catalytic reaction	368:387	a catalytic reaction	368:387	The generation of small sized metal nanoparticles which acts as catalyst is prerequisite to progress of a catalytic reaction.
24738363	0	83	theme	chitosan-silver	20:34	arg1	composite					49:57	In-situ and ex-situ chitosan-silver nanoparticle composite	0:57	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.	0:114	In-situ and ex-situ chitosan-silver nanoparticle composite: comparison of storage/release and catalytic properties.
24738363	13	84	theme	efficient	2059:2067	arg1	catalysis					2069:2077	efficient catalysis	2059:2077	efficient catalysis	2059:2077	The CH-Ag-E gel also showed reusability with efficient catalysis.
24738363	1	85	from	storage	129:135	arg1	gel					182:184	chitosan gel	173:184	chitosan gel	173:184	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	9	86	theme	Ag	1345:1346	arg1	NPs					1348:1350	Ag NPs	1345:1350	Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I	1345:1575	In addition to efficient swelling properties the CH-Ag-E can also act as store house of Ag NPs that can be used to catalyze the reduction of 4-Nitrophenol (4-NP) to 4-Aminophenol (4-AP) as Ag NPs of this composite can be easily extracted just by treating with sodium borohydride which is not possible in case of CH-Ag-I.
24738363	11	87	theme	NPs	1814:1816	arg1	size					1799:1802	the size	1795:1802	the size of the Ag NPs loaded in the gel (CH-Ag-E)	1795:1844	The reduction reaction catalyzed by such Ag NPs follow zero order kinetics and dependent on the size of the Ag NPs loaded in the gel (CH-Ag-E) as well as on the amount of the gel used.
24738363	10	88	theme	reaction	1594:1601	arg1	rate					1582:1585	The rate	1578:1585	The rate of the reaction	1578:1601	The rate of the reaction increases upto 10 fold when CH-Ag-E nanocomposite gel is used as catalyst in comparison to CH-Ag-I.
24738363	10	89	used	used	1660:1663	arg2	catalyst					1668:1675	catalyst	1668:1675	catalyst in comparison to CH-Ag-I	1668:1700	The rate of the reaction increases upto 10 fold when CH-Ag-E nanocomposite gel is used as catalyst in comparison to CH-Ag-I.
24738363	10	89	used	used	1660:1663	arg2	gel					1653:1655	CH-Ag-E nanocomposite gel	1631:1655	CH-Ag-E nanocomposite gel	1631:1655	The rate of the reaction increases upto 10 fold when CH-Ag-E nanocomposite gel is used as catalyst in comparison to CH-Ag-I.
24738363	10	90	theme	CH-Ag-E	1631:1637	arg1	catalyst					1668:1675	catalyst	1668:1675	catalyst in comparison to CH-Ag-I	1668:1700	The rate of the reaction increases upto 10 fold when CH-Ag-E nanocomposite gel is used as catalyst in comparison to CH-Ag-I.
24738363	10	90	theme	CH-Ag-E	1631:1637	arg1	gel					1653:1655	CH-Ag-E nanocomposite gel	1631:1655	CH-Ag-E nanocomposite gel	1631:1655	The rate of the reaction increases upto 10 fold when CH-Ag-E nanocomposite gel is used as catalyst in comparison to CH-Ag-I.
24738363	6	91	theme	NPs	838:840	arg1	solutions					842:850	Ag NPs solutions	835:850	Ag NPs solutions	835:850	And, in second approach already prepared chitosan hydrogel was placed in Ag NPs solutions, resulting in adsorption of Ag NPs and thus forming chitosan-Ag-ex-situ (CH-Ag-E) nanocomposite gel.
24738363	1	92	theme	catalytic	217:225	arg1	processes					237:245	catalytic reduction processes	217:245	catalytic reduction processes	217:245	In this work storage of silver nanoparticles (Ag NPs) in chitosan gel and its subsequent release for catalytic reduction processes is investigated.
24738363	12	93	dep	effective	1960:1968	arg1	smaller					1902:1908	smaller	1902:1908	smaller	1902:1908	We found that smaller is the size of the loaded Ag NPs in CH-Ag-E, more effective it is in catalyzing the reduction reaction.
24738363	12	93	dep	effective	1960:1968	arg1	size					1917:1920	the size	1913:1920	the size of the loaded Ag NPs in CH-Ag-E	1913:1952	We found that smaller is the size of the loaded Ag NPs in CH-Ag-E, more effective it is in catalyzing the reduction reaction.
24738363	13	94	theme	CH-Ag-E	2018:2024	arg1	gel					2026:2028	The CH-Ag-E gel	2014:2028	The CH-Ag-E gel	2014:2028	The CH-Ag-E gel also showed reusability with efficient catalysis.
24419797	6	0	theme	thermal	907:913	arg1	transitions					915:925	thermal transitions	907:925	thermal transitions	907:925	Thermal stability was examined as half-life at 60 °C and by recording of thermal transitions by circular dichroism.
24419797	3	1	theme	combinatorial	471:483	arg1	approach					505:512	a combinatorial protein engineering approach	469:512	a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions	469:574	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	2	2	theme	α-1,4	210:214	arg1	bonds					216:220	α-1,4 bonds	210:220	α-1,4 bonds	210:220	-) catalyze cleavage of α-1,4 bonds between rhamnose and galacturonic acid in the backbone of pectins by β-elimination.
24419797	4	3	theme	protein	651:657	arg1	PoPMuSiC					678:685	PoPMuSiC	678:685	PoPMuSiC	678:685	These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT).
24419797	4	3	theme	protein	651:657	arg1	changes					669:675	protein stability changes	651:675	protein stability changes (PoPMuSiC)	651:686	These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT).
24419797	7	4	theme	Pichia	1095:1100	arg1	pastoris					1102:1109	Pichia pastoris	1095:1109	Pichia pastoris	1095:1109	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	3	5	theme	acid	557:560	arg1	substitutions					562:574	single amino acid substitutions	544:574	single amino acid substitutions	544:574	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	9	6	theme	structural	1566:1575	arg1	analysis					1577:1584	the structural analysis	1562:1584	the structural analysis	1562:1584	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	9	7	theme	charged	1627:1633	arg1	acids					1641:1645	charged amino acids	1627:1645	charged amino acids	1627:1645	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	8	8	theme	thermal	1242:1248	arg1	stability					1250:1258	thermal stability	1242:1258	thermal stability at 60 °C	1242:1267	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	3	9	theme	single	544:549	arg1	substitutions					562:574	single amino acid substitutions	544:574	single amino acid substitutions	544:574	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	9	10	theme	acids	1641:1645	arg1	mutation					1615:1622	especially mutation	1604:1622	especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops	1604:1690	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	8	11	theme	single	1395:1400	arg1	G326E					1441:1445	G326E	1441:1445	G326E	1441:1445	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	11	theme	single	1395:1400	arg1	mutations					1413:1421	single amino acid mutations	1395:1421	single amino acid mutations (E434L, G55V, and G326E)	1395:1446	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	11	theme	single	1395:1400	arg1	G55V					1431:1434	G55V	1431:1434	G55V	1431:1434	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	3	12	from	improvement	337:347	arg1	DSM					429:431	DSM 13/ATCC14580	429:444	DSM 13/ATCC14580	429:444	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	3	12	from	improvement	337:347	arg1	licheniformis					414:426	Bacillus licheniformis	405:426	Bacillus licheniformis (DSM 13/ATCC14580)	405:445	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	2	13	theme	bonds	216:220	arg1	cleavage					198:205	cleavage	198:205	cleavage of α-1,4 bonds between rhamnose and galacturonic acid	198:259	-) catalyze cleavage of α-1,4 bonds between rhamnose and galacturonic acid in the backbone of pectins by β-elimination.
24419797	8	14	from	improvement	1227:1237	arg1	stability					1250:1258	thermal stability	1242:1258	thermal stability at 60 °C	1242:1267	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	14	from	improvement	1227:1237	arg1	°C					1266:1267	60 °C	1263:1267	60 °C	1263:1267	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	5	15	theme	single	816:821	arg1	mutations					823:831	two to seven individually favorable single mutations	780:831	two to seven individually favorable single mutations	780:831	The second-generation mutants involved combinations of two to seven individually favorable single mutations.
24419797	9	16	theme	hydrophobic	1650:1660	arg1	ones					1662:1665	hydrophobic ones	1650:1665	hydrophobic ones	1650:1665	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	8	17	theme	acid	1408:1411	arg1	G326E					1441:1445	G326E	1441:1445	G326E	1441:1445	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	17	theme	acid	1408:1411	arg1	mutations					1413:1421	single amino acid mutations	1395:1421	single amino acid mutations (E434L, G55V, and G326E)	1395:1446	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	17	theme	acid	1408:1411	arg1	G55V					1431:1434	G55V	1431:1434	G55V	1431:1434	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	4	18	theme	iterative	701:709	arg1	B-FIT					717:721	B-FIT	717:721	B-FIT	717:721	These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT).
24419797	4	18	theme	iterative	701:709	arg1	test					711:714	B-factor iterative test	692:714	B-factor iterative test (B-FIT)	692:722	These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT).
24419797	1	19	theme	Rhamnogalacturonan	135:152	arg1	lyases					156:161	Rhamnogalacturonan I lyases	135:161	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2	135:184	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.
24419797	1	19	theme	Rhamnogalacturonan	135:152	arg1	lyases					168:173	RGI lyases	164:173	RGI lyases	164:173	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.
24419797	7	20	theme	wild-type	1037:1045	arg1	lyase					1051:1055	the wild-type RGI lyase	1033:1055	the wild-type RGI lyase in Bacillus subtilis	1033:1076	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	2	21	theme	galacturonic	243:254	arg1	acid					256:259	galacturonic acid	243:259	galacturonic acid	243:259	-) catalyze cleavage of α-1,4 bonds between rhamnose and galacturonic acid in the backbone of pectins by β-elimination.
24419797	7	22	theme	thermal	989:995	arg1	stability					997:1005	thermal stability	989:1005	thermal stability	989:1005	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	3	23	theme	present	313:319	arg1	study					321:325	the present study	309:325	the present study	309:325	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	0	24	theme	thermostable	10:21	arg1	mutants					48:54	thermostable rhamnogalacturonan lyase mutants	10:54	thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis	10:82	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.
24419797	1	25	theme	RGI	164:166	arg1	lyases					156:161	Rhamnogalacturonan I lyases	135:161	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2	135:184	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.
24419797	1	25	theme	RGI	164:166	arg1	lyases					168:173	RGI lyases	164:173	RGI lyases	164:173	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.
24419797	9	26	theme	favorable	1701:1709	arg1	effects					1729:1735	favorable thermal stability effects	1701:1735	favorable thermal stability effects	1701:1735	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	7	27	theme	expressed	1197:1205	arg1	enzyme					1207:1212	the P. pastoris expressed enzyme	1181:1212	the P. pastoris expressed enzyme	1181:1212	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	9	28	theme	stability	1719:1727	arg1	effects					1729:1735	favorable thermal stability effects	1701:1735	favorable thermal stability effects	1701:1735	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	0	29	from	Design	0:5	arg1	licheniformis					70:82	Bacillus licheniformis	61:82	Bacillus licheniformis	61:82	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.
24419797	8	30	theme	T	1315:1315	arg1	m					1317:1317	T m	1315:1317	T m of the enzyme mutants	1315:1339	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	9	31	theme	B.	1504:1505	arg1	lyase					1535:1539	the B. licheniformis wild-type RGI lyase	1500:1539	the B. licheniformis wild-type RGI lyase	1500:1539	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	0	32	theme	targeted	102:109	arg1	mutations					124:132	targeted single point mutations	102:132	targeted single point mutations	102:132	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.
24419797	8	33	from	°C	1266:1267	arg1	improvement					1227:1237	A ~ twofold improvement	1215:1237	A ~ twofold improvement	1215:1237	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	33	from	°C	1266:1267	arg1	stability					1250:1258	thermal stability	1242:1258	thermal stability at 60 °C	1242:1267	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	0	34	theme	point	118:122	arg1	mutations					124:132	targeted single point mutations	102:132	targeted single point mutations	102:132	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.
24419797	8	35	from	increases	1302:1310	arg1	m					1317:1317	T m	1315:1317	T m of the enzyme mutants	1315:1339	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	9	36	theme	crystal	1479:1485	arg1	structure					1487:1495	The crystal structure	1475:1495	The crystal structure of the B. licheniformis wild-type RGI lyase	1475:1539	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	7	37	theme	enzyme	1207:1212	arg1	glycosylation					1164:1176	glycosylation	1164:1176	glycosylation of the P. pastoris expressed enzyme	1164:1212	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	8	38	theme	enzyme	1326:1331	arg1	mutants					1333:1339	the enzyme mutants	1322:1339	the enzyme mutants	1322:1339	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	3	39	theme	lyase	394:398	arg1	thermostability					356:370	the thermostability	352:370	the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580)	352:445	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	5	40	theme	favorable	806:814	arg1	mutations					823:831	two to seven individually favorable single mutations	780:831	two to seven individually favorable single mutations	780:831	The second-generation mutants involved combinations of two to seven individually favorable single mutations.
24419797	7	41	theme	negative	1145:1152	arg1	result					1154:1159	a negative result	1143:1159	a negative result of glycosylation of the P. pastoris expressed enzyme	1143:1212	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	7	41	theme	negative	1145:1152	arg1	effect					1117:1122	this effect	1112:1122	this effect	1112:1122	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	7	42	from	increment	976:984	arg1	stability					997:1005	thermal stability	989:1005	thermal stability	989:1005	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	8	43	theme	~	1217:1217	arg1	improvement					1227:1237	A ~ twofold improvement	1215:1237	A ~ twofold improvement	1215:1237	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	44	dep	mutations	1413:1421	arg1	G326E					1441:1445	G326E	1441:1445	G326E	1441:1445	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	44	dep	mutations	1413:1421	arg1	mutations					1413:1421	single amino acid mutations	1395:1421	single amino acid mutations (E434L, G55V, and G326E)	1395:1446	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	44	dep	mutations	1413:1421	arg1	G55V					1431:1434	G55V	1431:1434	G55V	1431:1434	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	0	45	theme	lyase	42:46	arg1	mutants					48:54	thermostable rhamnogalacturonan lyase mutants	10:54	thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis	10:82	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.
24419797	9	46	theme	wild-type	1521:1529	arg1	lyase					1535:1539	the B. licheniformis wild-type RGI lyase	1500:1539	the B. licheniformis wild-type RGI lyase	1500:1539	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	3	47	theme	PL	377:378	arg1	lyase					394:398	a PL family 11 RGI lyase	375:398	a PL family 11 RGI lyase	375:398	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	9	48	theme	lyase	1535:1539	arg1	structure					1487:1495	The crystal structure	1475:1495	The crystal structure of the B. licheniformis wild-type RGI lyase	1475:1539	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	8	49	theme	stabilizing	1372:1382	arg1	effects					1384:1390	additive stabilizing effects	1363:1390	additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E)	1363:1446	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	6	50	theme	Thermal	834:840	arg1	stability					842:850	Thermal stability	834:850	Thermal stability	834:850	Thermal stability was examined as half-life at 60 °C and by recording of thermal transitions by circular dichroism.
24419797	6	50	theme	Thermal	834:840	arg1	half-life					868:876	half-life	868:876	half-life	868:876	Thermal stability was examined as half-life at 60 °C and by recording of thermal transitions by circular dichroism.
24419797	5	51	theme	mutations	823:831	arg1	combinations					764:775	combinations	764:775	combinations of two to seven individually favorable single mutations	764:831	The second-generation mutants involved combinations of two to seven individually favorable single mutations.
24419797	3	52	theme	protein	485:491	arg1	approach					505:512	a combinatorial protein engineering approach	469:512	a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions	469:574	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	6	53	theme	transitions	915:925	arg1	recording					894:902	recording	894:902	recording of thermal transitions	894:925	Thermal stability was examined as half-life at 60 °C and by recording of thermal transitions by circular dichroism.
24419797	7	54	from	lyase	1051:1055	arg1	subtilis					1069:1076	Bacillus subtilis	1060:1076	Bacillus subtilis	1060:1076	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	3	55	from	licheniformis	414:426	arg1	improvement					337:347	targeted improvement	328:347	targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580)	328:445	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	3	55	from	licheniformis	414:426	arg1	thermostability					356:370	the thermostability	352:370	the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580)	352:445	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	5	56	dep	mutations	823:831	arg1	to					784:785	to	784:785	to	784:785	The second-generation mutants involved combinations of two to seven individually favorable single mutations.
24419797	3	57	theme	additive	524:531	arg1	effects					533:539	additive effects	524:539	additive effects of single amino acid substitutions	524:574	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	0	58	from	licheniformis	70:82	arg1	mutants					48:54	thermostable rhamnogalacturonan lyase mutants	10:54	thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis	10:82	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.
24419797	0	58	from	licheniformis	70:82	arg1	Design					0:5	Design	0:5	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.	0:133	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.
24419797	3	59	theme	amino	551:555	arg1	substitutions					562:574	single amino acid substitutions	544:574	single amino acid substitutions	544:574	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	9	60	from	mutation	1615:1622	arg1	loops					1686:1690	surface-exposed loops	1670:1690	surface-exposed loops	1670:1690	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	4	61	theme	stability	659:667	arg1	PoPMuSiC					678:685	PoPMuSiC	678:685	PoPMuSiC	678:685	These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT).
24419797	4	61	theme	stability	659:667	arg1	changes					669:675	protein stability changes	651:675	protein stability changes (PoPMuSiC)	651:686	These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT).
24419797	9	62	theme	amino	1635:1639	arg1	acids					1641:1645	charged amino acids	1627:1645	charged amino acids	1627:1645	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	8	63	theme	amino	1402:1406	arg1	G326E					1441:1445	G326E	1441:1445	G326E	1441:1445	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	63	theme	amino	1402:1406	arg1	mutations					1413:1421	single amino acid mutations	1395:1421	single amino acid mutations (E434L, G55V, and G326E)	1395:1446	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	63	theme	amino	1402:1406	arg1	G55V					1431:1434	G55V	1431:1434	G55V	1431:1434	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	4	64	theme	consensus	608:616	arg1	approach					618:625	a consensus approach	606:625	a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT)	606:722	These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT).
24419797	8	65	theme	twofold	1219:1225	arg1	improvement					1227:1237	A ~ twofold improvement	1215:1237	A ~ twofold improvement	1215:1237	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	8	66	theme	mutations	1413:1421	arg1	effects					1384:1390	additive stabilizing effects	1363:1390	additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E)	1363:1446	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	7	67	theme	RGI	1047:1049	arg1	lyase					1051:1055	the wild-type RGI lyase	1033:1055	the wild-type RGI lyase in Bacillus subtilis	1033:1076	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	3	68	theme	substitutions	562:574	arg1	effects					533:539	additive effects	524:539	additive effects of single amino acid substitutions	524:574	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	6	69	theme	circular	930:937	arg1	dichroism					939:947	circular dichroism	930:947	circular dichroism	930:947	Thermal stability was examined as half-life at 60 °C and by recording of thermal transitions by circular dichroism.
24419797	4	70	theme	B-factor	692:699	arg1	B-FIT					717:721	B-FIT	717:721	B-FIT	717:721	These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT).
24419797	4	70	theme	B-factor	692:699	arg1	test					711:714	B-factor iterative test	692:714	B-factor iterative test (B-FIT)	692:722	These were selected by using a consensus approach together with assessing protein stability changes (PoPMuSiC) and B-factor iterative test (B-FIT).
24419797	1	71	theme	I	154:154	arg1	lyases					156:161	Rhamnogalacturonan I lyases	135:161	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2	135:184	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.
24419797	1	71	theme	I	154:154	arg1	lyases					168:173	RGI lyases	164:173	RGI lyases	164:173	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.
24419797	9	72	theme	surface-exposed	1670:1684	arg1	loops					1686:1690	surface-exposed loops	1670:1690	surface-exposed loops	1670:1690	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	5	73	theme	second-generation	729:745	arg1	mutants					747:753	The second-generation mutants	725:753	The second-generation mutants	725:753	The second-generation mutants involved combinations of two to seven individually favorable single mutations.
24419797	9	74	dep	B.	1504:1505	arg1	licheniformis					1507:1519	licheniformis	1507:1519	licheniformis	1507:1519	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	7	75	theme	P.	1185:1186	arg1	enzyme					1207:1212	the P. pastoris expressed enzyme	1181:1212	the P. pastoris expressed enzyme	1181:1212	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	9	76	theme	thermal	1711:1717	arg1	effects					1729:1735	favorable thermal stability effects	1701:1735	favorable thermal stability effects	1701:1735	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	8	77	theme	significant	1290:1300	arg1	increases					1302:1310	less significant increases	1285:1310	less significant increases in T m of the enzyme mutants	1285:1339	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	0	78	theme	single	111:116	arg1	mutations					124:132	targeted single point mutations	102:132	targeted single point mutations	102:132	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.
24419797	7	79	theme	biggest	968:974	arg1	increment					976:984	the biggest increment	964:984	the biggest increment in thermal stability	964:1005	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	2	80	theme	pectins	280:286	arg1	backbone					268:275	the backbone	264:275	the backbone of pectins	264:286	-) catalyze cleavage of α-1,4 bonds between rhamnose and galacturonic acid in the backbone of pectins by β-elimination.
24419797	1	81	dep	lyases	156:161	arg1	EC					177:178	EC 4.2.2	177:184	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2	135:184	Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.
24419797	0	82	theme	mutations	124:132	arg1	combination					87:97	combination	87:97	combination of targeted single point mutations	87:132	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.
24419797	3	83	theme	targeted	328:335	arg1	improvement					337:347	targeted improvement	328:347	targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580)	328:445	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	8	84	theme	wild	1464:1467	arg1	type					1469:1472	the wild type	1460:1472	the wild type	1460:1472	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	7	85	gly	glycosylation	1164:1176	arg1	enzyme					1207:1212	the P. pastoris expressed enzyme	1181:1212	the P. pastoris expressed enzyme	1181:1212	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	7	86	theme	glycosylation	1164:1176	arg1	result					1154:1159	a negative result	1143:1159	a negative result of glycosylation of the P. pastoris expressed enzyme	1143:1212	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	7	86	theme	glycosylation	1164:1176	arg1	effect					1117:1122	this effect	1112:1122	this effect	1112:1122	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	0	87	theme	rhamnogalacturonan	23:40	arg1	mutants					48:54	thermostable rhamnogalacturonan lyase mutants	10:54	thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis	10:82	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.
24419797	3	88	theme	thermostability	356:370	arg1	improvement					337:347	targeted improvement	328:347	targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580)	328:445	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	8	89	theme	mutants	1333:1339	arg1	m					1317:1317	T m	1315:1317	T m of the enzyme mutants	1315:1339	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	0	90	theme	mutants	48:54	arg1	Design					0:5	Design	0:5	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.	0:133	Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations.
24419797	3	91	theme	RGI	390:392	arg1	lyase					394:398	a PL family 11 RGI lyase	375:398	a PL family 11 RGI lyase	375:398	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	9	92	theme	RGI	1531:1533	arg1	lyase					1535:1539	the B. licheniformis wild-type RGI lyase	1500:1539	the B. licheniformis wild-type RGI lyase	1500:1539	The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino acids to hydrophobic ones in surface-exposed loops produced favorable thermal stability effects.
24419797	3	93	theme	family	380:385	arg1	lyase					394:398	a PL family 11 RGI lyase	375:398	a PL family 11 RGI lyase	375:398	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
24419797	7	94	dep	P.	1185:1186	arg1	pastoris					1188:1195	pastoris	1188:1195	pastoris	1188:1195	Surprisingly, the biggest increment in thermal stability was achieved by producing the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme.
24419797	8	95	theme	additive	1363:1370	arg1	effects					1384:1390	additive stabilizing effects	1363:1390	additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E)	1363:1446	A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in T m of the enzyme mutants, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type.
24419797	3	96	theme	engineering	493:503	arg1	approach					505:512	a combinatorial protein engineering approach	469:512	a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions	469:574	In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM 13/ATCC14580) was examined by using a combinatorial protein engineering approach exploring additive effects of single amino acid substitutions.
25740179	8	0	theme	easy	1437:1440	arg1	comparisons					1452:1462	easy and quick comparisons	1437:1462	easy and quick comparisons among these different aromatic plants regarding nutritional purposes	1437:1531	The results obtained in the present systematization study will allow the readers to perform easy and quick comparisons among these different aromatic plants regarding nutritional purposes.
25740179	8	1	theme	different	1476:1484	arg1	plants					1495:1500	these different aromatic plants	1470:1500	these different aromatic plants	1470:1500	The results obtained in the present systematization study will allow the readers to perform easy and quick comparisons among these different aromatic plants regarding nutritional purposes.
25740179	3	2	theme	herbal	469:474	arg1	infusions					476:484	herbal infusions	469:484	herbal infusions	469:484	In the present study, 39 species of condiments and/or herbal infusions were assessed in order to provide scientific information concerning their nutritional value and energetic contribution; furthermore, the fatty acids composition was also evaluated.
25740179	4	3	located	detected	822:829	arg1	condiments					842:851	all the condiments	834:851	all the condiments	834:851	Carbohydrates were the most abundant compounds in the condiments that also revealed a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments.
25740179	4	3	located	detected	822:829	arg2	glucose					791:797	glucose	791:797	glucose	791:797	Carbohydrates were the most abundant compounds in the condiments that also revealed a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments.
25740179	4	3	located	detected	822:829	arg2	sugars					769:774	sugars	769:774	sugars with fructose	769:788	Carbohydrates were the most abundant compounds in the condiments that also revealed a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments.
25740179	1	4	theme	herbal	222:227	arg1	infusions					229:237	herbal infusions	222:237	herbal infusions	222:237	Aromatic plants have been used worldwide in human diet to improve the flavor and taste of meals or as herbal infusions.
25740179	5	5	theme	fatty	868:872	arg1	acids					874:878	fatty acids	868:878	fatty acids	868:878	In respect to fatty acids, PUFA were prevalent with the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids.
25740179	3	6	theme	infusions	476:484	arg1	species					440:446	39 species	437:446	39 species of condiments and/or herbal infusions	437:484	In the present study, 39 species of condiments and/or herbal infusions were assessed in order to provide scientific information concerning their nutritional value and energetic contribution; furthermore, the fatty acids composition was also evaluated.
25740179	3	7	theme	acids	629:633	arg1	composition					635:645	the fatty acids composition	619:645	the fatty acids composition	619:645	In the present study, 39 species of condiments and/or herbal infusions were assessed in order to provide scientific information concerning their nutritional value and energetic contribution; furthermore, the fatty acids composition was also evaluated.
25740179	7	8	theme	balanced	1302:1309	arg1	diet					1339:1342	a balanced and diversified low caloric diet	1300:1342	a balanced and diversified low caloric diet	1300:1342	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	5	9	theme	fatty	995:999	arg1	acids					1001:1005	the different 32 detected fatty acids	969:1005	the different 32 detected fatty acids	969:1005	In respect to fatty acids, PUFA were prevalent with the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids.
25740179	4	10	theme	varied	753:758	arg1	range					760:764	a varied range	751:764	a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments	751:851	Carbohydrates were the most abundant compounds in the condiments that also revealed a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments.
25740179	7	11	theme	energetic	1150:1158	arg1	low					1214:1216	low	1214:1216	low	1214:1216	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	7	11	theme	energetic	1150:1158	arg1	value					1160:1164	the energetic value	1146:1164	the energetic value of the condiments and herbal infusions	1146:1203	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	5	12	from	respect	857:863	arg1	prevalent					891:899	prevalent	891:899	prevalent	891:899	In respect to fatty acids, PUFA were prevalent with the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids.
25740179	5	13	theme	linoleic	932:939	arg1	acids					957:961	linoleic and α-linolenic acids	932:961	linoleic and α-linolenic acids	932:961	In respect to fatty acids, PUFA were prevalent with the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids.
25740179	4	14	with	sugars	769:774	arg1	fructose					781:788	fructose	781:788	fructose	781:788	Carbohydrates were the most abundant compounds in the condiments that also revealed a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments.
25740179	3	15	theme	condiments	451:460	arg1	species					440:446	39 species	437:446	39 species of condiments and/or herbal infusions	437:484	In the present study, 39 species of condiments and/or herbal infusions were assessed in order to provide scientific information concerning their nutritional value and energetic contribution; furthermore, the fatty acids composition was also evaluated.
25740179	7	16	theme	caloric	1331:1337	arg1	diet					1339:1342	a balanced and diversified low caloric diet	1300:1342	a balanced and diversified low caloric diet	1300:1342	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	7	17	theme	nutritional	1249:1259	arg1	properties					1261:1270	good nutritional properties	1244:1270	good nutritional properties that make them suitable for a balanced and diversified low caloric diet	1244:1342	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	1	18	used	used	146:149	arg2	plants					129:134	Aromatic plants	120:134	Aromatic plants	120:134	Aromatic plants have been used worldwide in human diet to improve the flavor and taste of meals or as herbal infusions.
25740179	1	19	theme	human	164:168	arg1	diet					170:173	human diet	164:173	human diet	164:173	Aromatic plants have been used worldwide in human diet to improve the flavor and taste of meals or as herbal infusions.
25740179	0	20	theme	Herbal	102:107	arg1	Infusions					109:117	Herbal Infusions	102:117	Herbal Infusions	102:117	A Comparison of the Nutritional Contribution of Thirty-nine Aromatic Plants used as Condiments and/or Herbal Infusions.
25740179	8	21	theme	aromatic	1486:1493	arg1	plants					1495:1500	these different aromatic plants	1470:1500	these different aromatic plants	1470:1500	The results obtained in the present systematization study will allow the readers to perform easy and quick comparisons among these different aromatic plants regarding nutritional purposes.
25740179	5	22	with	prevalent	891:899	arg1	contribution					916:927	the great contribution	906:927	the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids	906:1005	In respect to fatty acids, PUFA were prevalent with the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids.
25740179	2	23	used	used	292:295	arg2	plants					276:281	these plants	270:281	these plants	270:281	Beyond the culinary purposes, these plants are also used for their medicinal purposes, as antimicrobial, anti-inflammatory, antimutagenic and anti-carcinogenic, among others.
25740179	5	24	theme	α-linolenic	945:955	arg1	acids					957:961	linoleic and α-linolenic acids	932:961	linoleic and α-linolenic acids	932:961	In respect to fatty acids, PUFA were prevalent with the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids.
25740179	8	25	theme	nutritional	1512:1522	arg1	purposes					1524:1531	nutritional purposes	1512:1531	nutritional purposes	1512:1531	The results obtained in the present systematization study will allow the readers to perform easy and quick comparisons among these different aromatic plants regarding nutritional purposes.
25740179	5	26	from	prevalent	891:899	arg1	respect					857:863	respect	857:863	respect to fatty acids	857:878	In respect to fatty acids, PUFA were prevalent with the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids.
25740179	6	27	with	sugars	1056:1061	arg1	most					1068:1071	most	1068:1071	most	1068:1071	The herbal infusions revealed low quantities of sugars with most of the plants revealing fructose, glucose and sucrose.
25740179	6	27	with	sugars	1056:1061	arg1	plants					1080:1085	the plants	1076:1085	the plants revealing fructose	1076:1104	The herbal infusions revealed low quantities of sugars with most of the plants revealing fructose, glucose and sucrose.
25740179	6	28	theme	low	1038:1040	arg1	quantities					1042:1051	low quantities	1038:1051	low quantities of sugars with most of the plants revealing fructose, glucose and sucrose	1038:1125	The herbal infusions revealed low quantities of sugars with most of the plants revealing fructose, glucose and sucrose.
25740179	7	29	theme	general	1133:1139	arg1	way					1141:1143	a general way	1131:1143	a general way	1131:1143	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	3	30	theme	scientific	520:529	arg1	information					531:541	scientific information	520:541	scientific information concerning their nutritional value and energetic contribution	520:603	In the present study, 39 species of condiments and/or herbal infusions were assessed in order to provide scientific information concerning their nutritional value and energetic contribution; furthermore, the fatty acids composition was also evaluated.
25740179	7	31	theme	good	1244:1247	arg1	properties					1261:1270	good nutritional properties	1244:1270	good nutritional properties that make them suitable for a balanced and diversified low caloric diet	1244:1342	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	2	32	theme	culinary	251:258	arg1	purposes					260:267	the culinary purposes	247:267	the culinary purposes	247:267	Beyond the culinary purposes, these plants are also used for their medicinal purposes, as antimicrobial, anti-inflammatory, antimutagenic and anti-carcinogenic, among others.
25740179	0	33	used	used	76:79	arg2	Comparison					2:11	A Comparison	0:11	A Comparison of the Nutritional Contribution of Thirty-nine Aromatic Plants	0:74	A Comparison of the Nutritional Contribution of Thirty-nine Aromatic Plants used as Condiments and/or Herbal Infusions.
25740179	6	34	theme	sugars	1056:1061	arg1	quantities					1042:1051	low quantities	1038:1051	low quantities of sugars with most of the plants revealing fructose, glucose and sucrose	1038:1125	The herbal infusions revealed low quantities of sugars with most of the plants revealing fructose, glucose and sucrose.
25740179	6	35	theme	herbal	1012:1017	arg1	infusions					1019:1027	The herbal infusions	1008:1027	The herbal infusions	1008:1027	The herbal infusions revealed low quantities of sugars with most of the plants revealing fructose, glucose and sucrose.
25740179	0	36	theme	Contribution	32:43	arg1	Comparison					2:11	A Comparison	0:11	A Comparison of the Nutritional Contribution of Thirty-nine Aromatic Plants	0:74	A Comparison of the Nutritional Contribution of Thirty-nine Aromatic Plants used as Condiments and/or Herbal Infusions.
25740179	4	37	theme	abundant	695:702	arg1	compounds					704:712	the most abundant compounds	686:712	the most abundant compounds	686:712	Carbohydrates were the most abundant compounds in the condiments that also revealed a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments.
25740179	4	37	theme	abundant	695:702	arg1	Carbohydrates					667:679	Carbohydrates	667:679	Carbohydrates	667:679	Carbohydrates were the most abundant compounds in the condiments that also revealed a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments.
25740179	8	38	theme	systematization	1381:1395	arg1	study					1397:1401	the present systematization study	1369:1401	the present systematization study	1369:1401	The results obtained in the present systematization study will allow the readers to perform easy and quick comparisons among these different aromatic plants regarding nutritional purposes.
25740179	7	39	theme	low	1327:1329	arg1	diet					1339:1342	a balanced and diversified low caloric diet	1300:1342	a balanced and diversified low caloric diet	1300:1342	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	7	40	from	low	1214:1216	arg1	way					1141:1143	a general way	1131:1143	a general way	1131:1143	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	3	41	theme	present	422:428	arg1	study					430:434	the present study	418:434	the present study	418:434	In the present study, 39 species of condiments and/or herbal infusions were assessed in order to provide scientific information concerning their nutritional value and energetic contribution; furthermore, the fatty acids composition was also evaluated.
25740179	0	42	theme	Nutritional	20:30	arg1	Contribution					32:43	the Nutritional Contribution	16:43	the Nutritional Contribution of Thirty-nine Aromatic Plants	16:74	A Comparison of the Nutritional Contribution of Thirty-nine Aromatic Plants used as Condiments and/or Herbal Infusions.
25740179	3	43	theme	energetic	582:590	arg1	contribution					592:603	energetic contribution	582:603	energetic contribution	582:603	In the present study, 39 species of condiments and/or herbal infusions were assessed in order to provide scientific information concerning their nutritional value and energetic contribution; furthermore, the fatty acids composition was also evaluated.
25740179	7	44	theme	herbal	1188:1193	arg1	infusions					1195:1203	the condiments and herbal infusions	1169:1203	infusions	1195:1203	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	0	45	theme	Thirty-nine	48:58	arg1	Plants					69:74	Thirty-nine Aromatic Plants	48:74	Thirty-nine Aromatic Plants	48:74	A Comparison of the Nutritional Contribution of Thirty-nine Aromatic Plants used as Condiments and/or Herbal Infusions.
25740179	3	46	theme	fatty	623:627	arg1	acids					629:633	the fatty acids	619:633	the fatty acids composition	619:645	In the present study, 39 species of condiments and/or herbal infusions were assessed in order to provide scientific information concerning their nutritional value and energetic contribution; furthermore, the fatty acids composition was also evaluated.
25740179	3	47	theme	nutritional	560:570	arg1	value					572:576	their nutritional value	554:576	their nutritional value	554:576	In the present study, 39 species of condiments and/or herbal infusions were assessed in order to provide scientific information concerning their nutritional value and energetic contribution; furthermore, the fatty acids composition was also evaluated.
25740179	2	48	theme	medicinal	307:315	arg1	purposes					317:324	their medicinal purposes	301:324	their medicinal purposes	301:324	Beyond the culinary purposes, these plants are also used for their medicinal purposes, as antimicrobial, anti-inflammatory, antimutagenic and anti-carcinogenic, among others.
25740179	8	49	theme	quick	1446:1450	arg1	comparisons					1452:1462	easy and quick comparisons	1437:1462	easy and quick comparisons among these different aromatic plants regarding nutritional purposes	1437:1531	The results obtained in the present systematization study will allow the readers to perform easy and quick comparisons among these different aromatic plants regarding nutritional purposes.
25740179	7	50	theme	diversified	1315:1325	arg1	diet					1339:1342	a balanced and diversified low caloric diet	1300:1342	a balanced and diversified low caloric diet	1300:1342	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	1	51	theme	Aromatic	120:127	arg1	plants					129:134	Aromatic plants	120:134	Aromatic plants	120:134	Aromatic plants have been used worldwide in human diet to improve the flavor and taste of meals or as herbal infusions.
25740179	5	52	theme	different	973:981	arg1	acids					1001:1005	the different 32 detected fatty acids	969:1005	the different 32 detected fatty acids	969:1005	In respect to fatty acids, PUFA were prevalent with the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids.
25740179	5	53	theme	acids	957:961	arg1	contribution					916:927	the great contribution	906:927	the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids	906:1005	In respect to fatty acids, PUFA were prevalent with the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids.
25740179	5	54	theme	detected	986:993	arg1	acids					1001:1005	the different 32 detected fatty acids	969:1005	the different 32 detected fatty acids	969:1005	In respect to fatty acids, PUFA were prevalent with the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids.
25740179	5	55	theme	great	910:914	arg1	contribution					916:927	the great contribution	906:927	the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids	906:1005	In respect to fatty acids, PUFA were prevalent with the great contribution of linoleic and α-linolenic acids among the different 32 detected fatty acids.
25740179	4	56	theme	sugars	769:774	arg1	range					760:764	a varied range	751:764	a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments	751:851	Carbohydrates were the most abundant compounds in the condiments that also revealed a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments.
25740179	4	57	from	compounds	704:712	arg1	condiments					721:730	the condiments	717:730	the condiments that also revealed a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments	717:851	Carbohydrates were the most abundant compounds in the condiments that also revealed a varied range of sugars with fructose, glucose, sucrose and trehalose detected in all the condiments.
25740179	1	58	theme	meals	210:214	arg1	flavor					190:195	flavor	190:195	flavor	190:195	Aromatic plants have been used worldwide in human diet to improve the flavor and taste of meals or as herbal infusions.
25740179	1	58	theme	meals	210:214	arg1	taste					201:205	taste	201:205	taste	201:205	Aromatic plants have been used worldwide in human diet to improve the flavor and taste of meals or as herbal infusions.
25740179	0	59	theme	Plants	69:74	arg1	Contribution					32:43	the Nutritional Contribution	16:43	the Nutritional Contribution of Thirty-nine Aromatic Plants	16:74	A Comparison of the Nutritional Contribution of Thirty-nine Aromatic Plants used as Condiments and/or Herbal Infusions.
25740179	8	60	theme	present	1373:1379	arg1	study					1397:1401	the present systematization study	1369:1401	the present systematization study	1369:1401	The results obtained in the present systematization study will allow the readers to perform easy and quick comparisons among these different aromatic plants regarding nutritional purposes.
25740179	7	61	theme	infusions	1195:1203	arg1	low					1214:1216	low	1214:1216	low	1214:1216	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	7	61	theme	infusions	1195:1203	arg1	value					1160:1164	the energetic value	1146:1164	the energetic value of the condiments and herbal infusions	1146:1203	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	7	62	theme	condiments	1173:1182	arg1	infusions					1195:1203	the condiments and herbal infusions	1169:1203	infusions	1195:1203	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	0	63	theme	Aromatic	60:67	arg1	Plants					69:74	Thirty-nine Aromatic Plants	48:74	Thirty-nine Aromatic Plants	48:74	A Comparison of the Nutritional Contribution of Thirty-nine Aromatic Plants used as Condiments and/or Herbal Infusions.
25740179	1	64	dep	flavor	190:195	arg1	the					186:188	the	186:188	the	186:188	Aromatic plants have been used worldwide in human diet to improve the flavor and taste of meals or as herbal infusions.
25740179	7	65	from	way	1141:1143	arg1	low					1214:1216	low	1214:1216	low	1214:1216	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
25740179	7	65	from	way	1141:1143	arg1	value					1160:1164	the energetic value	1146:1164	the energetic value of the condiments and herbal infusions	1146:1203	In a general way, the energetic value of the condiments and herbal infusions was very low and these plants revealed good nutritional properties that make them suitable for a balanced and diversified low caloric diet.
27270331	2	0	with	compatible	375:384	arg1	KPZ					425:427	KPZ	425:427	KPZ	425:427	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	2	0	with	compatible	375:384	arg1	Kardar-Parisi-Zhang					404:422	the standard Kardar-Parisi-Zhang	391:422	the standard Kardar-Parisi-Zhang (KPZ)	391:428	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	2	1	theme	front	347:351	arg1	dynamics					363:370	their 2D front roughness dynamics	338:370	their 2D front roughness dynamics	338:370	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	11	2	theme	individual	1817:1826	arg1	motility					1833:1840	individual cell motility	1817:1840	individual cell motility	1817:1840	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	6	3	with	change	1055:1060	arg1	time					1067:1070	time	1067:1070	time	1067:1070	For small N, the average radial front velocity and its change with time depend on MC concentration.
27270331	3	4	from	significant	634:644	arg1	cases					552:556	both cases	547:556	both cases	547:556	In both cases, the influence of a non-uniform distribution of the colony constituents was significant.
27270331	11	5	theme	models	1968:1973	arg1	predictions					1920:1930	the predictions	1916:1930	the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ	1916:2071	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	2	6	theme	cell	309:312	arg1	colonies					314:321	large cell colonies	303:321	large cell colonies	303:321	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	13	7	theme	colony	2262:2267	arg1	dynamics					2285:2292	the 2D colony front roughness dynamics	2255:2292	the 2D colony front roughness dynamics	2255:2292	Seemingly, these results show a possible way of linking the cellular Potts models and the 2D colony front roughness dynamics.
27270331	11	8	theme	dynamics	2026:2033	arg1	transition					1992:2001	the observed transition	1979:2001	the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ	1979:2071	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	11	8	theme	dynamics	2026:2033	arg1	models					1968:1973	the extended cellular Potts models	1940:1973	the extended cellular Potts models	1940:1973	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	4	9	theme	systems	720:726	arg1	dynamics					702:709	the overall dynamics	690:709	the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells	690:818	These results encouraged us to investigate the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells.
27270331	10	10	theme	velocity	1677:1684	arg1	field					1686:1690	the corresponding velocity field	1659:1690	the corresponding velocity field	1659:1690	The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium.
27270331	1	11	theme	microscopic	196:206	arg1	approaches					219:228	microscopic and global approaches	196:228	microscopic and global approaches	196:228	To deal with complex systems, microscopic and global approaches become of particular interest.
27270331	11	12	theme	front	2010:2014	arg1	dynamics					2026:2033	the front roughness dynamics	2006:2033	the front roughness dynamics	2006:2033	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	11	13	theme	Local	1776:1780	arg1	distribution					1800:1811	Local average cell size distribution	1776:1811	Local average cell size distribution	1776:1811	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	11	14	with	consistent	1900:1909	arg1	predictions					1920:1930	the predictions	1916:1930	the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ	1916:2071	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	8	15	dep	proceeds	1314:1321	arg1	latter					1451:1456	latter	1451:1456	latter	1451:1456	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	8	15	dep	proceeds	1314:1321	arg1	former					1371:1376	former	1371:1376	former	1371:1376	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	0	16	theme	culture	151:157	arg1	media					159:163	both plain and methylcellulose-containing culture media	109:163	both plain and methylcellulose-containing culture media	109:163	Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media.
27270331	13	17	theme	2D	2259:2260	arg1	dynamics					2285:2292	the 2D colony front roughness dynamics	2255:2292	the 2D colony front roughness dynamics	2255:2292	Seemingly, these results show a possible way of linking the cellular Potts models and the 2D colony front roughness dynamics.
27270331	11	18	theme	extended	1944:1951	arg1	models					1968:1973	the extended cellular Potts models	1940:1973	the extended cellular Potts models	1940:1973	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	8	19	theme	Colony	1274:1279	arg1	spreading					1281:1289	Colony spreading	1274:1289	Colony spreading in MC-containing media	1274:1312	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	11	20	theme	cellular	1953:1960	arg1	models					1968:1973	the extended cellular Potts models	1940:1973	the extended cellular Potts models	1940:1973	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	5	21	theme	quasi-circular	895:908	arg1	fronts					934:939	quasi-circular and quasi-linear growth fronts	895:939	quasi-circular and quasi-linear growth fronts	895:939	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	2	22	from	dynamics	291:298	arg1	results					274:280	Our previous results	261:280	Our previous results from the dynamics of large cell colonies	261:321	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	6	23	theme	small	1004:1008	arg1	N					1010:1010	small N	1004:1010	small N	1004:1010	For small N, the average radial front velocity and its change with time depend on MC concentration.
27270331	11	24	from	KPZ	2051:2053	arg1	predictions					1920:1930	the predictions	1916:1930	the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ	1916:2071	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	11	24	from	KPZ	2051:2053	arg1	transition					1992:2001	the observed transition	1979:2001	the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ	1979:2071	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	11	24	from	KPZ	2051:2053	arg1	models					1968:1973	the extended cellular Potts models	1940:1973	the extended cellular Potts models	1940:1973	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	7	25	theme	cells	1187:1191	arg1	enlargement					1172:1182	the local enlargement	1162:1182	the local enlargement of cells	1162:1191	MC in the medium interferes with cell mitosis, contributes to the local enlargement of cells, and increases the distribution of spatio-temporal cell density heterogeneities.
27270331	3	26	theme	distribution	590:601	arg1	significant					634:644	significant	634:644	significant	634:644	In both cases, the influence of a non-uniform distribution of the colony constituents was significant.
27270331	3	26	theme	distribution	590:601	arg1	influence					563:571	the influence	559:571	the influence of a non-uniform distribution of the colony constituents	559:628	In both cases, the influence of a non-uniform distribution of the colony constituents was significant.
27270331	5	27	theme	cell	863:866	arg1	populations					868:878	different cell populations	853:878	different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media	853:980	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	5	27	theme	cell	863:866	arg1	N					881:881	N	881:881	N	881:881	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	2	28	theme	methylcellulose	479:493	arg1	media					523:527	plain or methylcellulose (MC)-containing gel culture media	470:527	plain or methylcellulose (MC)-containing gel culture media	470:527	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	10	29	dep	enlarged	1715:1722	arg1	slow-moving					1725:1735	slow-moving	1725:1735	slow-moving	1725:1735	The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium.
27270331	0	30	theme	spreading	70:78	arg1	dynamics					80:87	the 2D spreading dynamics	63:87	the 2D spreading dynamics	63:87	Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media.
27270331	2	31	theme	culture	515:521	arg1	media					523:527	plain or methylcellulose (MC)-containing gel culture media	470:527	plain or methylcellulose (MC)-containing gel culture media	470:527	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	5	32	theme	plain	944:948	arg1	media					976:980	plain and MC-containing culture media	944:980	plain and MC-containing culture media	944:980	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	2	33	theme	-containing	499:509	arg1	media					523:527	plain or methylcellulose (MC)-containing gel culture media	470:527	plain or methylcellulose (MC)-containing gel culture media	470:527	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	0	34	from	effects	52:58	arg1	dynamics					80:87	the 2D spreading dynamics	63:87	the 2D spreading dynamics	63:87	Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media.
27270331	8	35	theme	main	1333:1336	arg1	effects					1348:1354	two main quenching effects	1329:1354	two main quenching effects	1329:1354	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	8	35	theme	main	1333:1336	arg1	II					1363:1364	II	1363:1364	II	1363:1364	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	8	35	theme	main	1333:1336	arg1	I					1357:1357	I	1357:1357	I	1357:1357	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	2	36	theme	standard	395:402	arg1	KPZ					425:427	KPZ	425:427	KPZ	425:427	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	2	36	theme	standard	395:402	arg1	Kardar-Parisi-Zhang					404:422	the standard Kardar-Parisi-Zhang	391:422	the standard Kardar-Parisi-Zhang (KPZ)	391:428	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	0	37	theme	colonies	97:104	arg1	effects					52:58	effects	52:58	effects on the 2D spreading dynamics of cell colonies	52:104	Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media.
27270331	5	38	theme	MC-containing	954:966	arg1	media					976:980	plain and MC-containing culture media	944:980	plain and MC-containing culture media	944:980	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	2	39	theme	KPZ	446:448	arg1	equations					450:458	the quenched KPZ equations	433:458	the quenched KPZ equations	433:458	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	8	40	theme	enlarged	1488:1495	arg1	domains					1508:1514	enlarged local cell domains	1488:1514	enlarged local cell domains	1488:1514	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	10	41	theme	cell	1599:1602	arg1	motility					1604:1611	cell motility	1599:1611	cell motility	1599:1611	The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium.
27270331	0	42	theme	Spatio-temporal	0:14	arg1	morphology					16:25	Spatio-temporal morphology	0:25	Spatio-temporal morphology	0:25	Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media.
27270331	5	43	theme	quasi-linear	914:925	arg1	fronts					934:939	quasi-circular and quasi-linear growth fronts	895:939	quasi-circular and quasi-linear growth fronts	895:939	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	6	44	theme	front	1032:1036	arg1	velocity					1038:1045	the average radial front velocity	1013:1045	the average radial front velocity	1013:1045	For small N, the average radial front velocity and its change with time depend on MC concentration.
27270331	8	45	theme	cell	1503:1506	arg1	domains					1508:1514	enlarged local cell domains	1488:1514	enlarged local cell domains	1488:1514	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	12	46	theme	quenched-KPZ	2146:2157	arg1	equation					2159:2166	the quenched-KPZ equation	2142:2166	the quenched-KPZ equation	2142:2166	In this case, quenching effects I and II cooperate and give rise to the quenched-KPZ equation.
27270331	6	47	with	velocity	1038:1045	arg1	time					1067:1070	time	1067:1070	time	1067:1070	For small N, the average radial front velocity and its change with time depend on MC concentration.
27270331	9	48	theme	colony	1530:1535	arg1	spreading					1537:1545	colony spreading	1530:1545	colony spreading	1530:1545	For large N, colony spreading occurs at constant velocity.
27270331	6	49	theme	average	1017:1023	arg1	velocity					1038:1045	the average radial front velocity	1013:1045	the average radial front velocity	1013:1045	For small N, the average radial front velocity and its change with time depend on MC concentration.
27270331	2	50	theme	plain	470:474	arg1	media					523:527	plain or methylcellulose (MC)-containing gel culture media	470:527	plain or methylcellulose (MC)-containing gel culture media	470:527	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	7	51	theme	heterogeneities	1257:1271	arg1	distribution					1212:1223	the distribution	1208:1223	the distribution of spatio-temporal cell density heterogeneities	1208:1271	MC in the medium interferes with cell mitosis, contributes to the local enlargement of cells, and increases the distribution of spatio-temporal cell density heterogeneities.
27270331	5	52	with	colonies	839:846	arg1	populations					868:878	different cell populations	853:878	different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media	853:980	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	5	52	with	colonies	839:846	arg1	N					881:881	N	881:881	N	881:881	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	3	53	theme	constituents	617:628	arg1	distribution					590:601	a non-uniform distribution	576:601	a non-uniform distribution of the colony constituents	576:628	In both cases, the influence of a non-uniform distribution of the colony constituents was significant.
27270331	4	54	theme	single	807:812	arg1	cells					814:818	single cells	807:818	single cells	807:818	These results encouraged us to investigate the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells.
27270331	2	55	theme	2D	344:345	arg1	dynamics					363:370	their 2D front roughness dynamics	338:370	their 2D front roughness dynamics	338:370	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	9	56	theme	constant	1557:1564	arg1	velocity					1566:1573	constant velocity	1557:1573	constant velocity	1557:1573	For large N, colony spreading occurs at constant velocity.
27270331	7	57	theme	cell	1244:1247	arg1	heterogeneities					1257:1271	spatio-temporal cell density heterogeneities	1228:1271	spatio-temporal cell density heterogeneities	1228:1271	MC in the medium interferes with cell mitosis, contributes to the local enlargement of cells, and increases the distribution of spatio-temporal cell density heterogeneities.
27270331	10	58	theme	medium	1768:1773	arg1	effect					1705:1710	the effect	1701:1710	the effect of enlarged, slow-moving cells	1701:1741	The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium.
27270331	10	58	theme	medium	1768:1773	arg1	structure					1751:1759	the structure	1747:1759	the structure of the medium	1747:1773	The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium.
27270331	11	59	theme	cell	1790:1793	arg1	distribution					1800:1811	Local average cell size distribution	1776:1811	Local average cell size distribution	1776:1811	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	13	60	theme	roughness	2275:2283	arg1	dynamics					2285:2292	the 2D colony front roughness dynamics	2255:2292	the 2D colony front roughness dynamics	2255:2292	Seemingly, these results show a possible way of linking the cellular Potts models and the 2D colony front roughness dynamics.
27270331	2	61	from	media	523:527	arg1	compatible					375:384	compatible	375:384	compatible	375:384	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	11	62	theme	motility	1833:1840	arg1	data					1842:1845	Local average cell size distribution and individual cell motility data	1776:1845	Local average cell size distribution and individual cell motility data from plain and MC-containing media	1776:1880	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	2	63	theme	roughness	353:361	arg1	dynamics					363:370	their 2D front roughness dynamics	338:370	their 2D front roughness dynamics	338:370	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	10	64	theme	enlarged	1715:1722	arg1	cells					1737:1741	enlarged, slow-moving cells	1715:1741	enlarged, slow-moving cells	1715:1741	The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium.
27270331	11	65	theme	quenched	2060:2067	arg1	KPZ					2069:2071	a quenched KPZ	2058:2071	a quenched KPZ	2058:2071	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	10	66	theme	corresponding	1663:1675	arg1	field					1686:1690	the corresponding velocity field	1659:1690	the corresponding velocity field	1659:1690	The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium.
27270331	11	67	theme	Potts	1962:1966	arg1	models					1968:1973	the extended cellular Potts models	1940:1973	the extended cellular Potts models	1940:1973	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	12	68	dep	effects	2098:2104	arg1	I					2106:2106	I	2106:2106	I	2106:2106	In this case, quenching effects I and II cooperate and give rise to the quenched-KPZ equation.
27270331	12	68	dep	effects	2098:2104	arg1	effects					2098:2104	quenching effects I and II	2088:2113	quenching effects I and II	2088:2113	In this case, quenching effects I and II cooperate and give rise to the quenched-KPZ equation.
27270331	12	68	dep	effects	2098:2104	arg1	II					2112:2113	II	2112:2113	II	2112:2113	In this case, quenching effects I and II cooperate and give rise to the quenched-KPZ equation.
27270331	4	69	theme	cells	814:818	arg1	motility					795:802	the motility	791:802	the motility of single cells	791:818	These results encouraged us to investigate the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells.
27270331	4	69	theme	cells	814:818	arg1	morphology					744:753	morphology	744:753	morphology	744:753	These results encouraged us to investigate the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells.
27270331	4	69	theme	cells	814:818	arg1	rate					781:784	the duplication rate	765:784	the duplication rate	765:784	These results encouraged us to investigate the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells.
27270331	4	69	theme	cells	814:818	arg1	size					759:762	size	759:762	size	759:762	These results encouraged us to investigate the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells.
27270331	2	70	theme	colonies	314:321	arg1	dynamics					291:298	the dynamics	287:298	the dynamics of large cell colonies	287:321	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	1	71	theme	complex	179:185	arg1	systems					187:193	complex systems	179:193	complex systems	179:193	To deal with complex systems, microscopic and global approaches become of particular interest.
27270331	0	72	theme	plain	114:118	arg1	media					159:163	both plain and methylcellulose-containing culture media	109:163	both plain and methylcellulose-containing culture media	109:163	Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media.
27270331	2	73	theme	large	303:307	arg1	colonies					314:321	large cell colonies	303:321	large cell colonies	303:321	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	10	74	theme	cells	1737:1741	arg1	effect					1705:1710	the effect	1701:1710	the effect of enlarged, slow-moving cells	1701:1741	The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium.
27270331	10	74	theme	cells	1737:1741	arg1	structure					1751:1759	the structure	1747:1759	the structure of the medium	1747:1773	The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium.
27270331	8	75	theme	culture	1402:1408	arg1	medium					1410:1415	the culture medium composition and structure	1398:1441	the culture medium composition and structure	1398:1441	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	11	76	from	predictions	1920:1930	arg1	KPZ					2051:2053	a standard KPZ	2040:2053	a standard KPZ to a quenched KPZ	2040:2071	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	0	77	theme	methylcellulose-containing	124:149	arg1	media					159:163	both plain and methylcellulose-containing culture media	109:163	both plain and methylcellulose-containing culture media	109:163	Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media.
27270331	13	78	theme	Potts	2238:2242	arg1	models					2244:2249	the cellular Potts models	2225:2249	the cellular Potts models	2225:2249	Seemingly, these results show a possible way of linking the cellular Potts models and the 2D colony front roughness dynamics.
27270331	11	79	theme	roughness	2016:2024	arg1	dynamics					2026:2033	the front roughness dynamics	2006:2033	the front roughness dynamics	2006:2033	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	11	80	from	media	1876:1880	arg1	data					1842:1845	Local average cell size distribution and individual cell motility data	1776:1845	Local average cell size distribution and individual cell motility data from plain and MC-containing media	1776:1880	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	11	81	theme	average	1782:1788	arg1	distribution					1800:1811	Local average cell size distribution	1776:1811	Local average cell size distribution	1776:1811	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	13	82	theme	possible	2201:2208	arg1	way					2210:2212	a possible way	2199:2212	a possible way of linking the cellular Potts models and the 2D colony front roughness dynamics	2199:2292	Seemingly, these results show a possible way of linking the cellular Potts models and the 2D colony front roughness dynamics.
27270331	4	83	theme	overall	694:700	arg1	dynamics					702:709	the overall dynamics	690:709	the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells	690:818	These results encouraged us to investigate the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells.
27270331	13	84	theme	front	2269:2273	arg1	dynamics					2285:2292	the 2D colony front roughness dynamics	2255:2292	the 2D colony front roughness dynamics	2255:2292	Seemingly, these results show a possible way of linking the cellular Potts models and the 2D colony front roughness dynamics.
27270331	2	85	theme	previous	265:272	arg1	results					274:280	Our previous results	261:280	Our previous results from the dynamics of large cell colonies	261:321	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	11	86	theme	observed	1983:1990	arg1	transition					1992:2001	the observed transition	1979:2001	the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ	1979:2071	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	11	87	theme	standard	2042:2049	arg1	KPZ					2051:2053	a standard KPZ	2040:2053	a standard KPZ to a quenched KPZ	2040:2071	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	3	88	from	cases	552:556	arg1	significant					634:644	significant	634:644	significant	634:644	In both cases, the influence of a non-uniform distribution of the colony constituents was significant.
27270331	3	88	from	cases	552:556	arg1	influence					563:571	the influence	559:571	the influence of a non-uniform distribution of the colony constituents	559:628	In both cases, the influence of a non-uniform distribution of the colony constituents was significant.
27270331	0	89	theme	2D	67:68	arg1	dynamics					80:87	the 2D spreading dynamics	63:87	the 2D spreading dynamics	63:87	Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media.
27270331	3	90	theme	non-uniform	578:588	arg1	distribution					590:601	a non-uniform distribution	576:601	a non-uniform distribution of the colony constituents	576:628	In both cases, the influence of a non-uniform distribution of the colony constituents was significant.
27270331	5	91	theme	different	853:861	arg1	populations					868:878	different cell populations	853:878	different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media	853:980	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	5	91	theme	different	853:861	arg1	N					881:881	N	881:881	N	881:881	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	7	92	from	MC	1100:1101	arg1	medium					1110:1115	the medium	1106:1115	the medium	1106:1115	MC in the medium interferes with cell mitosis, contributes to the local enlargement of cells, and increases the distribution of spatio-temporal cell density heterogeneities.
27270331	7	93	theme	local	1166:1170	arg1	enlargement					1172:1182	the local enlargement	1162:1182	the local enlargement of cells	1162:1191	MC in the medium interferes with cell mitosis, contributes to the local enlargement of cells, and increases the distribution of spatio-temporal cell density heterogeneities.
27270331	11	94	theme	cell	1828:1831	arg1	motility					1833:1840	individual cell motility	1817:1840	individual cell motility	1817:1840	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	11	95	theme	distribution	1800:1811	arg1	data					1842:1845	Local average cell size distribution and individual cell motility data	1776:1845	Local average cell size distribution and individual cell motility data from plain and MC-containing media	1776:1880	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	8	96	theme	MC-containing	1294:1306	arg1	media					1308:1312	MC-containing media	1294:1312	MC-containing media	1294:1312	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	0	97	theme	cell	92:95	arg1	colonies					97:104	cell colonies	92:104	cell colonies	92:104	Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media.
27270331	12	98	theme	quenching	2088:2096	arg1	I					2106:2106	I	2106:2106	I	2106:2106	In this case, quenching effects I and II cooperate and give rise to the quenched-KPZ equation.
27270331	12	98	theme	quenching	2088:2096	arg1	effects					2098:2104	quenching effects I and II	2088:2113	quenching effects I and II	2088:2113	In this case, quenching effects I and II cooperate and give rise to the quenched-KPZ equation.
27270331	12	98	theme	quenching	2088:2096	arg1	II					2112:2113	II	2112:2113	II	2112:2113	In this case, quenching effects I and II cooperate and give rise to the quenched-KPZ equation.
27270331	11	99	theme	transition	1992:2001	arg1	predictions					1920:1930	the predictions	1916:1930	the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ	1916:2071	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	2	100	theme	gel	511:513	arg1	media					523:527	plain or methylcellulose (MC)-containing gel culture media	470:527	plain or methylcellulose (MC)-containing gel culture media	470:527	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	13	101	theme	cellular	2229:2236	arg1	models					2244:2249	the cellular Potts models	2225:2249	the cellular Potts models	2225:2249	Seemingly, these results show a possible way of linking the cellular Potts models and the 2D colony front roughness dynamics.
27270331	8	102	theme	local	1497:1501	arg1	domains					1508:1514	enlarged local cell domains	1488:1514	enlarged local cell domains	1488:1514	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	10	103	theme	motility	1604:1611	arg1	characteristics					1580:1594	The characteristics	1576:1594	The characteristics	1576:1594	The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium.
27270331	7	104	theme	cell	1133:1136	arg1	mitosis					1138:1144	cell mitosis	1133:1144	cell mitosis	1133:1144	MC in the medium interferes with cell mitosis, contributes to the local enlargement of cells, and increases the distribution of spatio-temporal cell density heterogeneities.
27270331	9	105	theme	large	1521:1525	arg1	N					1527:1527	large N	1521:1527	large N	1521:1527	For large N, colony spreading occurs at constant velocity.
27270331	2	106	theme	quenched	437:444	arg1	equations					450:458	the quenched KPZ equations	433:458	the quenched KPZ equations	433:458	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	8	107	theme	domains	1508:1514	arg1	distribution					1472:1483	the distribution	1468:1483	the distribution of enlarged local cell domains	1468:1514	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	8	108	theme	quenching	1338:1346	arg1	effects					1348:1354	two main quenching effects	1329:1354	two main quenching effects	1329:1354	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	8	108	theme	quenching	1338:1346	arg1	II					1363:1364	II	1363:1364	II	1363:1364	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	8	108	theme	quenching	1338:1346	arg1	I					1357:1357	I	1357:1357	I	1357:1357	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	11	109	theme	plain	1852:1856	arg1	media					1876:1880	plain and MC-containing media	1852:1880	plain and MC-containing media	1852:1880	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	8	110	from	spreading	1281:1289	arg1	media					1308:1312	MC-containing media	1294:1312	MC-containing media	1294:1312	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	5	111	theme	growth	927:932	arg1	fronts					934:939	quasi-circular and quasi-linear growth fronts	895:939	quasi-circular and quasi-linear growth fronts	895:939	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	6	112	theme	radial	1025:1030	arg1	velocity					1038:1045	the average radial front velocity	1013:1045	the average radial front velocity	1013:1045	For small N, the average radial front velocity and its change with time depend on MC concentration.
27270331	7	113	theme	density	1249:1255	arg1	heterogeneities					1257:1271	spatio-temporal cell density heterogeneities	1228:1271	spatio-temporal cell density heterogeneities	1228:1271	MC in the medium interferes with cell mitosis, contributes to the local enlargement of cells, and increases the distribution of spatio-temporal cell density heterogeneities.
27270331	3	114	theme	colony	610:615	arg1	constituents					617:628	the colony constituents	606:628	the colony constituents	606:628	In both cases, the influence of a non-uniform distribution of the colony constituents was significant.
27270331	2	115	dep	Kardar-Parisi-Zhang	404:422	arg1	either					460:465	either	460:465	either	460:465	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	8	116	dep	medium	1410:1415	arg1	structure					1433:1441	structure	1433:1441	structure	1433:1441	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	8	116	dep	medium	1410:1415	arg1	composition					1417:1427	composition	1417:1427	composition	1417:1427	Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains.
27270331	2	117	from	compatible	375:384	arg1	media					523:527	plain or methylcellulose (MC)-containing gel culture media	470:527	plain or methylcellulose (MC)-containing gel culture media	470:527	Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar-Parisi-Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively.
27270331	7	118	theme	spatio-temporal	1228:1242	arg1	heterogeneities					1257:1271	spatio-temporal cell density heterogeneities	1228:1271	spatio-temporal cell density heterogeneities	1228:1271	MC in the medium interferes with cell mitosis, contributes to the local enlargement of cells, and increases the distribution of spatio-temporal cell density heterogeneities.
27270331	1	119	with	deal	169:172	arg1	systems					187:193	complex systems	179:193	complex systems	179:193	To deal with complex systems, microscopic and global approaches become of particular interest.
27270331	5	120	theme	culture	968:974	arg1	media					976:980	plain and MC-containing culture media	944:980	plain and MC-containing culture media	944:980	For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated.
27270331	6	121	theme	MC	1082:1083	arg1	concentration					1085:1097	MC concentration	1082:1097	MC concentration	1082:1097	For small N, the average radial front velocity and its change with time depend on MC concentration.
27270331	4	122	theme	duplication	769:779	arg1	rate					781:784	the duplication rate	765:784	the duplication rate	765:784	These results encouraged us to investigate the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells.
27270331	11	123	theme	size	1795:1798	arg1	distribution					1800:1811	Local average cell size distribution	1776:1811	Local average cell size distribution	1776:1811	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	1	124	theme	global	212:217	arg1	approaches					219:228	microscopic and global approaches	196:228	microscopic and global approaches	196:228	To deal with complex systems, microscopic and global approaches become of particular interest.
27270331	11	125	theme	MC-containing	1862:1874	arg1	media					1876:1880	plain and MC-containing media	1852:1880	plain and MC-containing media	1852:1880	Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ.
27270331	1	126	theme	particular	240:249	arg1	interest					251:258	particular interest	240:258	particular interest	240:258	To deal with complex systems, microscopic and global approaches become of particular interest.
24558059	10	0	theme	loading	2455:2461	arg1	influence					2442:2450	the influence	2438:2450	the influence of loading on binding kinetics	2438:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	0	theme	loading	2455:2461	arg1	evolution					2255:2263	the evolution	2251:2263	the evolution of construct dimensions with growth	2251:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	0	theme	loading	2455:2461	arg1	influence					2306:2314	the influence	2302:2314	the influence of solute and solid matrix electric charge on the transport of cytokines	2302:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	0	theme	loading	2455:2461	arg1	influence					2136:2144	the influence	2132:2144	the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties	2132:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	0	theme	loading	2455:2461	arg1	influence					2394:2402	the influence	2390:2402	the influence of binding kinetics on transport	2390:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	0	theme	loading	2455:2461	arg1	response					2512:2519	the differential growth response	2488:2519	the differential growth response to dynamically loaded versus free-swelling culture conditions	2488:2581	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	4	1	theme	solid	1104:1108	arg1	matrix					1110:1115	the solid matrix	1100:1115	the solid matrix	1100:1115	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	8	2	theme	tissue	1757:1762	arg1	engineering					1764:1774	An illustrative tissue engineering	1741:1774	An illustrative tissue engineering analysis	1741:1783	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	6	3	theme	reactions	1558:1566	arg1	number					1535:1540	any number	1531:1540	any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly	1531:1635	To achieve these objectives, this treatment identifies the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents while accommodating any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly.
24558059	2	4	from	applications	349:360	arg1	charged					432:438	charged	432:438	charged	432:438	In many biological applications, the reactants and products of chemical reactions may be electrically charged, and these charge effects may produce driving forces and constraints that significantly influence outcomes.
24558059	10	5	theme	construct	2268:2276	arg1	dimensions					2278:2287	construct dimensions	2268:2287	construct dimensions	2268:2287	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	6	with	influence	2306:2314	arg1	growth					2294:2299	growth	2294:2299	growth	2294:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	7	with	influence	2442:2450	arg1	growth					2294:2299	growth	2294:2299	growth	2294:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	3	8	theme	solid	775:779	arg1	matrix					781:786	a deformable porous hydrated solid matrix	746:786	a deformable porous hydrated solid matrix	746:786	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	8	9	theme	sulfate	1870:1876	arg1	deposition					1844:1853	the deposition	1840:1853	the deposition of chondroitin sulfate, a charged solid-bound molecular species	1840:1917	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	4	10	dep	deposition	866:875	arg1	The					862:864	The	862:864	The	862:864	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	10	11	theme	binding	2466:2472	arg1	kinetics					2474:2481	binding kinetics	2466:2481	binding kinetics	2466:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	2	12	theme	many	333:336	arg1	applications					349:360	many biological applications	333:360	many biological applications	333:360	In many biological applications, the reactants and products of chemical reactions may be electrically charged, and these charge effects may produce driving forces and constraints that significantly influence outcomes.
24558059	1	13	theme	such	208:211	arg1	processes					213:221	such processes	208:221	such processes	208:221	Mechanobiological processes are rooted in mechanics and chemistry, and such processes may be modeled in a framework that couples their governing equations starting from fundamental principles.
24558059	10	14	theme	growth	2505:2510	arg1	response					2512:2519	the differential growth response	2488:2519	the differential growth response to dynamically loaded versus free-swelling culture conditions	2488:2581	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	15	theme	solid	2330:2334	arg1	charge					2352:2357	solid matrix electric charge	2330:2357	solid matrix electric charge	2330:2357	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	6	16	theme	computational	1404:1416	arg1	challenges					1418:1427	the specific theoretical and computational challenges	1375:1427	the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents	1375:1509	To achieve these objectives, this treatment identifies the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents while accommodating any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly.
24558059	3	17	theme	computational	587:599	arg1	implementation					601:614	computational implementation	587:614	computational implementation	587:614	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	8	18	theme	solid-bound	1889:1899	arg1	sulfate					1870:1876	chondroitin sulfate	1858:1876	chondroitin sulfate	1858:1876	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	8	18	theme	solid-bound	1889:1899	arg1	species					1911:1917	a charged solid-bound molecular species	1879:1917	a charged solid-bound molecular species	1879:1917	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	10	19	theme	solute	2319:2324	arg1	influence					2442:2450	the influence	2438:2450	the influence of loading on binding kinetics	2438:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	19	theme	solute	2319:2324	arg1	evolution					2255:2263	the evolution	2251:2263	the evolution of construct dimensions with growth	2251:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	19	theme	solute	2319:2324	arg1	influence					2306:2314	the influence	2302:2314	the influence of solute and solid matrix electric charge on the transport of cytokines	2302:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	19	theme	solute	2319:2324	arg1	influence					2136:2144	the influence	2132:2144	the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties	2132:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	19	theme	solute	2319:2324	arg1	influence					2394:2402	the influence	2390:2402	the influence of binding kinetics on transport	2390:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	19	theme	solute	2319:2324	arg1	response					2512:2519	the differential growth response	2488:2519	the differential growth response to dynamically loaded versus free-swelling culture conditions	2488:2581	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	3	20	theme	electric	844:851	arg1	charges					853:859	electric charges	844:859	electric charges	844:859	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	6	21	theme	theoretical	1388:1398	arg1	challenges					1418:1427	the specific theoretical and computational challenges	1375:1427	the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents	1375:1509	To achieve these objectives, this treatment identifies the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents while accommodating any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly.
24558059	3	22	theme	chemical	643:650	arg1	reactions					652:660	modeling chemical reactions	634:660	modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges	634:859	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	4	23	theme	matrix	967:972	arg1	remodeling					943:952	remodeling	943:952	remodeling	943:952	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	4	23	theme	matrix	967:972	arg1	growth					932:937	growth	932:937	growth	932:937	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	10	24	from	influence	2394:2402	arg1	transport					2427:2435	transport	2427:2435	transport	2427:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	24	from	influence	2394:2402	arg1	evolution					2178:2186	the evolution	2174:2186	the evolution of inhomogeneous tissue composition and mechanical properties	2174:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	24	from	influence	2394:2402	arg1	kinetics					2474:2481	binding kinetics	2466:2481	binding kinetics	2466:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	24	from	influence	2394:2402	arg1	transport					2366:2374	the transport	2362:2374	the transport of cytokines	2362:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	25	theme	availability	2158:2169	arg1	influence					2442:2450	the influence	2438:2450	the influence of loading on binding kinetics	2438:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	25	theme	availability	2158:2169	arg1	evolution					2255:2263	the evolution	2251:2263	the evolution of construct dimensions with growth	2251:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	25	theme	availability	2158:2169	arg1	influence					2306:2314	the influence	2302:2314	the influence of solute and solid matrix electric charge on the transport of cytokines	2302:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	25	theme	availability	2158:2169	arg1	influence					2136:2144	the influence	2132:2144	the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties	2132:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	25	theme	availability	2158:2169	arg1	influence					2394:2402	the influence	2390:2402	the influence of binding kinetics on transport	2390:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	25	theme	availability	2158:2169	arg1	response					2512:2519	the differential growth response	2488:2519	the differential growth response to dynamically loaded versus free-swelling culture conditions	2488:2581	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	1	26	theme	Mechanobiological	137:153	arg1	processes					155:163	Mechanobiological processes	137:163	Mechanobiological processes	137:163	Mechanobiological processes are rooted in mechanics and chemistry, and such processes may be modeled in a framework that couples their governing equations starting from fundamental principles.
24558059	10	27	theme	electric	2343:2350	arg1	charge					2352:2357	solid matrix electric charge	2330:2357	solid matrix electric charge	2330:2357	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	28	theme	tissue	2205:2210	arg1	composition					2212:2222	inhomogeneous tissue composition	2191:2222	inhomogeneous tissue composition	2191:2222	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	7	29	theme	closed-form	1707:1717	arg1	solutions					1730:1738	closed-form analytical solutions	1707:1738	closed-form analytical solutions	1707:1738	Several finite element verification problems are shown to agree with closed-form analytical solutions.
24558059	4	30	theme	osmotic	1032:1038	arg1	swelling					1040:1047	Donnan osmotic swelling	1025:1047	Donnan osmotic swelling	1025:1047	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	10	31	theme	cytokines	2379:2387	arg1	transport					2366:2374	the transport	2362:2374	the transport of cytokines	2362:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	6	32	theme	interacting	1466:1476	arg1	constituents					1498:1509	interacting neutral and charged constituents	1466:1509	interacting neutral and charged constituents	1466:1509	To achieve these objectives, this treatment identifies the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents while accommodating any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly.
24558059	5	33	theme	state	1173:1177	arg1	variable					1179:1186	a state variable	1171:1186	a state variable in the production rate of chemical reactions	1171:1231	This formulation incorporates the state of strain as a state variable in the production rate of chemical reactions, explicitly tying chemistry with mechanics for the purpose of modeling mechanobiology.
24558059	5	33	theme	state	1173:1177	arg1	state					1152:1156	the state	1148:1156	the state of strain	1148:1166	This formulation incorporates the state of strain as a state variable in the production rate of chemical reactions, explicitly tying chemistry with mechanics for the purpose of modeling mechanobiology.
24558059	10	34	theme	free-swelling	2550:2562	arg1	conditions					2572:2581	dynamically loaded versus free-swelling culture conditions	2524:2581	dynamically loaded versus free-swelling culture conditions	2524:2581	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	35	theme	properties	2239:2248	arg1	evolution					2178:2186	the evolution	2174:2186	the evolution of inhomogeneous tissue composition and mechanical properties	2174:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	6	36	theme	modeling	1438:1445	arg1	systems					1455:1461	modeling complex systems	1438:1461	modeling complex systems of interacting neutral and charged constituents	1438:1509	To achieve these objectives, this treatment identifies the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents while accommodating any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly.
24558059	10	37	from	influence	2136:2144	arg1	transport					2427:2435	transport	2427:2435	transport	2427:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	37	from	influence	2136:2144	arg1	evolution					2178:2186	the evolution	2174:2186	the evolution of inhomogeneous tissue composition and mechanical properties	2174:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	37	from	influence	2136:2144	arg1	kinetics					2474:2481	binding kinetics	2466:2481	binding kinetics	2466:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	37	from	influence	2136:2144	arg1	transport					2366:2374	the transport	2362:2374	the transport of cytokines	2362:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	0	38	theme	solid-bound	114:124	arg1	molecules					126:134	solid-bound molecules	114:134	solid-bound molecules	114:134	Computational modeling of chemical reactions and interstitial growth and remodeling involving charged solutes and solid-bound molecules.
24558059	3	39	with	mechanics	798:806	arg1	chemistry					813:821	chemistry	813:821	chemistry	813:821	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	4	40	theme	fixed	1091:1095	arg1	species					1083:1089	charged molecular species	1065:1089	charged molecular species fixed to the solid matrix	1065:1115	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	10	41	with	influence	2394:2402	arg1	growth					2294:2299	growth	2294:2299	growth	2294:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	0	42	theme	Computational	0:12	arg1	modeling					14:21	Computational modeling	0:21	Computational modeling of chemical reactions and interstitial growth and remodeling	0:82	Computational modeling of chemical reactions and interstitial growth and remodeling involving charged solutes and solid-bound molecules.
24558059	5	43	theme	reactions	1223:1231	arg1	rate					1206:1209	the production rate	1191:1209	the production rate of chemical reactions	1191:1231	This formulation incorporates the state of strain as a state variable in the production rate of chemical reactions, explicitly tying chemistry with mechanics for the purpose of modeling mechanobiology.
24558059	9	44	theme	program	1971:1977	arg1	www.febio.org					1987:1999	www.febio.org	1987:1999	www.febio.org	1987:1999	This implementation is released in the open-source program FEBio ( www.febio.org ).
24558059	9	44	theme	program	1971:1977	arg1	FEBio					1979:1983	the open-source program FEBio	1955:1983	the open-source program FEBio ( www.febio.org )	1955:2001	This implementation is released in the open-source program FEBio ( www.febio.org ).
24558059	10	45	theme	kinetics	2415:2422	arg1	influence					2442:2450	the influence	2438:2450	the influence of loading on binding kinetics	2438:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	45	theme	kinetics	2415:2422	arg1	evolution					2255:2263	the evolution	2251:2263	the evolution of construct dimensions with growth	2251:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	45	theme	kinetics	2415:2422	arg1	influence					2306:2314	the influence	2302:2314	the influence of solute and solid matrix electric charge on the transport of cytokines	2302:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	45	theme	kinetics	2415:2422	arg1	influence					2136:2144	the influence	2132:2144	the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties	2132:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	45	theme	kinetics	2415:2422	arg1	influence					2394:2402	the influence	2390:2402	the influence of binding kinetics on transport	2390:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	45	theme	kinetics	2415:2422	arg1	response					2512:2519	the differential growth response	2488:2519	the differential growth response to dynamically loaded versus free-swelling culture conditions	2488:2581	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	2	46	dep	reactants	367:375	arg1	the					363:365	the	363:365	the	363:365	In many biological applications, the reactants and products of chemical reactions may be electrically charged, and these charge effects may produce driving forces and constraints that significantly influence outcomes.
24558059	1	47	theme	fundamental	306:316	arg1	principles					318:327	fundamental principles	306:327	fundamental principles	306:327	Mechanobiological processes are rooted in mechanics and chemistry, and such processes may be modeled in a framework that couples their governing equations starting from fundamental principles.
24558059	7	48	theme	finite	1646:1651	arg1	problems					1674:1681	Several finite element verification problems	1638:1681	Several finite element verification problems	1638:1681	Several finite element verification problems are shown to agree with closed-form analytical solutions.
24558059	10	49	from	response	2512:2519	arg1	transport					2427:2435	transport	2427:2435	transport	2427:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	49	from	response	2512:2519	arg1	evolution					2178:2186	the evolution	2174:2186	the evolution of inhomogeneous tissue composition and mechanical properties	2174:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	49	from	response	2512:2519	arg1	kinetics					2474:2481	binding kinetics	2466:2481	binding kinetics	2466:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	49	from	response	2512:2519	arg1	transport					2366:2374	the transport	2362:2374	the transport of cytokines	2362:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	6	50	theme	constituents	1498:1509	arg1	systems					1455:1461	modeling complex systems	1438:1461	modeling complex systems of interacting neutral and charged constituents	1438:1509	To achieve these objectives, this treatment identifies the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents while accommodating any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly.
24558059	5	51	theme	production	1195:1204	arg1	rate					1206:1209	the production rate	1191:1209	the production rate of chemical reactions	1191:1231	This formulation incorporates the state of strain as a state variable in the production rate of chemical reactions, explicitly tying chemistry with mechanics for the purpose of modeling mechanobiology.
24558059	0	52	theme	reactions	35:43	arg1	modeling					14:21	Computational modeling	0:21	Computational modeling of chemical reactions and interstitial growth and remodeling	0:82	Computational modeling of chemical reactions and interstitial growth and remodeling involving charged solutes and solid-bound molecules.
24558059	4	53	theme	molecular	1073:1081	arg1	species					1083:1089	charged molecular species	1065:1089	charged molecular species fixed to the solid matrix	1065:1115	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	10	54	theme	culture	2564:2570	arg1	conditions					2572:2581	dynamically loaded versus free-swelling culture conditions	2524:2581	dynamically loaded versus free-swelling culture conditions	2524:2581	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	4	55	theme	molecules	903:911	arg1	deposition					866:875	deposition	866:875	deposition	866:875	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	4	55	theme	molecules	903:911	arg1	removal					880:886	removal	880:886	removal	880:886	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	10	56	theme	culture	2103:2109	arg1	systems					2111:2117	tissue engineering culture systems	2084:2117	tissue engineering culture systems	2084:2117	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	5	57	from	variable	1179:1186	arg1	rate					1206:1209	the production rate	1191:1209	the production rate of chemical reactions	1191:1231	This formulation incorporates the state of strain as a state variable in the production rate of chemical reactions, explicitly tying chemistry with mechanics for the purpose of modeling mechanobiology.
24558059	2	58	theme	driving	478:484	arg1	forces					486:491	driving forces	478:491	driving forces	478:491	In many biological applications, the reactants and products of chemical reactions may be electrically charged, and these charge effects may produce driving forces and constraints that significantly influence outcomes.
24558059	10	59	theme	loaded	2536:2541	arg1	conditions					2572:2581	dynamically loaded versus free-swelling culture conditions	2524:2581	dynamically loaded versus free-swelling culture conditions	2524:2581	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	3	60	theme	solid-bound	717:727	arg1	molecules					729:737	solid-bound molecules	717:737	solid-bound molecules	717:737	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	2	61	theme	chemical	393:400	arg1	reactions					402:410	chemical reactions	393:410	chemical reactions	393:410	In many biological applications, the reactants and products of chemical reactions may be electrically charged, and these charge effects may produce driving forces and constraints that significantly influence outcomes.
24558059	6	62	theme	charged	1490:1496	arg1	constituents					1498:1509	interacting neutral and charged constituents	1466:1509	interacting neutral and charged constituents	1466:1509	To achieve these objectives, this treatment identifies the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents while accommodating any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly.
24558059	3	63	theme	hydrated	766:773	arg1	matrix					781:786	a deformable porous hydrated solid matrix	746:786	a deformable porous hydrated solid matrix	746:786	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	10	64	with	response	2512:2519	arg1	growth					2294:2299	growth	2294:2299	growth	2294:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	65	theme	dimensions	2278:2287	arg1	influence					2442:2450	the influence	2438:2450	the influence of loading on binding kinetics	2438:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	65	theme	dimensions	2278:2287	arg1	evolution					2255:2263	the evolution	2251:2263	the evolution of construct dimensions with growth	2251:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	65	theme	dimensions	2278:2287	arg1	influence					2306:2314	the influence	2302:2314	the influence of solute and solid matrix electric charge on the transport of cytokines	2302:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	65	theme	dimensions	2278:2287	arg1	influence					2136:2144	the influence	2132:2144	the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties	2132:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	65	theme	dimensions	2278:2287	arg1	influence					2394:2402	the influence	2390:2402	the influence of binding kinetics on transport	2390:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	65	theme	dimensions	2278:2287	arg1	response					2512:2519	the differential growth response	2488:2519	the differential growth response to dynamically loaded versus free-swelling culture conditions	2488:2581	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	8	66	theme	illustrative	1744:1755	arg1	engineering					1764:1774	An illustrative tissue engineering	1741:1774	An illustrative tissue engineering analysis	1741:1783	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	3	67	theme	deformable	748:757	arg1	matrix					781:786	a deformable porous hydrated solid matrix	746:786	a deformable porous hydrated solid matrix	746:786	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	3	68	theme	novel	565:569	arg1	formulation					571:581	a novel formulation	563:581	a novel formulation	563:581	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	6	69	theme	simultaneous	1545:1556	arg1	reactions					1558:1566	simultaneous reactions	1545:1566	simultaneous reactions where reactants and products may be modeled explicitly or implicitly	1545:1635	To achieve these objectives, this treatment identifies the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents while accommodating any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly.
24558059	1	70	theme	governing	272:280	arg1	equations					282:290	their governing equations	266:290	their governing equations starting from fundamental principles	266:327	Mechanobiological processes are rooted in mechanics and chemistry, and such processes may be modeled in a framework that couples their governing equations starting from fundamental principles.
24558059	8	71	theme	engineering	1764:1774	arg1	analysis					1776:1783	An illustrative tissue engineering analysis	1741:1783	An illustrative tissue engineering analysis	1741:1783	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	3	72	theme	coupling	789:796	arg1	mechanics					798:806	coupling mechanics	789:806	coupling mechanics with chemistry	789:821	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	3	72	theme	coupling	789:796	arg1	solutes					705:711	charged solutes	697:711	charged solutes	697:711	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	0	73	theme	interstitial	49:60	arg1	growth					62:67	growth	62:67	growth	62:67	Computational modeling of chemical reactions and interstitial growth and remodeling involving charged solutes and solid-bound molecules.
24558059	10	74	theme	engineering	2091:2101	arg1	systems					2111:2117	tissue engineering culture systems	2084:2117	tissue engineering culture systems	2084:2117	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	2	75	theme	biological	338:347	arg1	applications					349:360	many biological applications	333:360	many biological applications	333:360	In many biological applications, the reactants and products of chemical reactions may be electrically charged, and these charge effects may produce driving forces and constraints that significantly influence outcomes.
24558059	8	76	theme	chondroitin	1858:1868	arg1	sulfate					1870:1876	chondroitin sulfate	1858:1876	chondroitin sulfate	1858:1876	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	8	76	theme	chondroitin	1858:1868	arg1	species					1911:1917	a charged solid-bound molecular species	1879:1917	a charged solid-bound molecular species	1879:1917	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	10	77	theme	matrix	2336:2341	arg1	charge					2352:2357	solid matrix electric charge	2330:2357	solid matrix electric charge	2330:2357	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	78	from	influence	2306:2314	arg1	transport					2427:2435	transport	2427:2435	transport	2427:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	78	from	influence	2306:2314	arg1	evolution					2178:2186	the evolution	2174:2186	the evolution of inhomogeneous tissue composition and mechanical properties	2174:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	78	from	influence	2306:2314	arg1	kinetics					2474:2481	binding kinetics	2466:2481	binding kinetics	2466:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	78	from	influence	2306:2314	arg1	transport					2366:2374	the transport	2362:2374	the transport of cytokines	2362:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	79	theme	differential	2492:2503	arg1	response					2512:2519	the differential growth response	2488:2519	the differential growth response to dynamically loaded versus free-swelling culture conditions	2488:2581	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	3	80	theme	modeling	634:641	arg1	reactions					652:660	modeling chemical reactions	634:660	modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges	634:859	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	8	81	theme	charged	1881:1887	arg1	sulfate					1870:1876	chondroitin sulfate	1858:1876	chondroitin sulfate	1858:1876	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	8	81	theme	charged	1881:1887	arg1	species					1911:1917	a charged solid-bound molecular species	1879:1917	a charged solid-bound molecular species	1879:1917	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	4	82	theme	volumetric	990:999	arg1	growth					1001:1006	volumetric growth	990:1006	volumetric growth	990:1006	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	6	83	theme	specific	1379:1386	arg1	challenges					1418:1427	the specific theoretical and computational challenges	1375:1427	the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents	1375:1509	To achieve these objectives, this treatment identifies the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents while accommodating any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly.
24558059	8	84	theme	molecular	1901:1909	arg1	sulfate					1870:1876	chondroitin sulfate	1858:1876	chondroitin sulfate	1858:1876	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	8	84	theme	molecular	1901:1909	arg1	species					1911:1917	a charged solid-bound molecular species	1879:1917	a charged solid-bound molecular species	1879:1917	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	7	85	theme	analytical	1719:1728	arg1	solutions					1730:1738	closed-form analytical solutions	1707:1738	closed-form analytical solutions	1707:1738	Several finite element verification problems are shown to agree with closed-form analytical solutions.
24558059	4	86	theme	solid	961:965	arg1	matrix					967:972	the solid matrix	957:972	the solid matrix	957:972	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	10	87	with	influence	2136:2144	arg1	growth					2294:2299	growth	2294:2299	growth	2294:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	88	theme	charge	2352:2357	arg1	influence					2442:2450	the influence	2438:2450	the influence of loading on binding kinetics	2438:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	88	theme	charge	2352:2357	arg1	evolution					2255:2263	the evolution	2251:2263	the evolution of construct dimensions with growth	2251:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	88	theme	charge	2352:2357	arg1	influence					2306:2314	the influence	2302:2314	the influence of solute and solid matrix electric charge on the transport of cytokines	2302:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	88	theme	charge	2352:2357	arg1	influence					2136:2144	the influence	2132:2144	the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties	2132:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	88	theme	charge	2352:2357	arg1	influence					2394:2402	the influence	2390:2402	the influence of binding kinetics on transport	2390:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	88	theme	charge	2352:2357	arg1	response					2512:2519	the differential growth response	2488:2519	the differential growth response to dynamically loaded versus free-swelling culture conditions	2488:2581	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	89	theme	nutrient	2149:2156	arg1	availability					2158:2169	nutrient availability	2149:2169	nutrient availability	2149:2169	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	3	90	theme	biological	665:674	arg1	tissues					676:682	biological tissues	665:682	biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges	665:859	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	10	91	theme	inhomogeneous	2191:2203	arg1	composition					2212:2222	inhomogeneous tissue composition	2191:2222	inhomogeneous tissue composition	2191:2222	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	6	92	theme	neutral	1478:1484	arg1	constituents					1498:1509	interacting neutral and charged constituents	1466:1509	interacting neutral and charged constituents	1466:1509	To achieve these objectives, this treatment identifies the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents while accommodating any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly.
24558059	4	93	theme	Donnan	1025:1030	arg1	swelling					1040:1047	Donnan osmotic swelling	1025:1047	Donnan osmotic swelling	1025:1047	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	5	94	theme	mechanobiology	1304:1317	arg1	purpose					1284:1290	the purpose	1280:1290	the purpose of modeling mechanobiology	1280:1317	This formulation incorporates the state of strain as a state variable in the production rate of chemical reactions, explicitly tying chemistry with mechanics for the purpose of modeling mechanobiology.
24558059	0	95	theme	charged	94:100	arg1	solutes					102:108	charged solutes	94:108	charged solutes	94:108	Computational modeling of chemical reactions and interstitial growth and remodeling involving charged solutes and solid-bound molecules.
24558059	10	96	theme	binding	2407:2413	arg1	kinetics					2415:2422	binding kinetics	2407:2422	binding kinetics	2407:2422	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	97	theme	mechanical	2228:2237	arg1	properties					2239:2248	mechanical properties	2228:2248	mechanical properties	2228:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	6	98	theme	complex	1447:1453	arg1	systems					1455:1461	modeling complex systems	1438:1461	modeling complex systems of interacting neutral and charged constituents	1438:1509	To achieve these objectives, this treatment identifies the specific theoretical and computational challenges faced in modeling complex systems of interacting neutral and charged constituents while accommodating any number of simultaneous reactions where reactants and products may be modeled explicitly or implicitly.
24558059	7	99	theme	verification	1661:1672	arg1	problems					1674:1681	Several finite element verification problems	1638:1681	Several finite element verification problems	1638:1681	Several finite element verification problems are shown to agree with closed-form analytical solutions.
24558059	3	100	from	reactions	652:660	arg1	tissues					676:682	biological tissues	665:682	biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges	665:859	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	10	101	theme	composition	2212:2222	arg1	evolution					2178:2186	the evolution	2174:2186	the evolution of inhomogeneous tissue composition and mechanical properties	2174:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	5	102	theme	strain	1161:1166	arg1	variable					1179:1186	a state variable	1171:1186	a state variable in the production rate of chemical reactions	1171:1231	This formulation incorporates the state of strain as a state variable in the production rate of chemical reactions, explicitly tying chemistry with mechanics for the purpose of modeling mechanobiology.
24558059	5	102	theme	strain	1161:1166	arg1	state					1152:1156	the state	1148:1156	the state of strain	1148:1166	This formulation incorporates the state of strain as a state variable in the production rate of chemical reactions, explicitly tying chemistry with mechanics for the purpose of modeling mechanobiology.
24558059	10	103	from	evolution	2255:2263	arg1	transport					2427:2435	transport	2427:2435	transport	2427:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	103	from	evolution	2255:2263	arg1	evolution					2178:2186	the evolution	2174:2186	the evolution of inhomogeneous tissue composition and mechanical properties	2174:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	103	from	evolution	2255:2263	arg1	kinetics					2474:2481	binding kinetics	2466:2481	binding kinetics	2466:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	103	from	evolution	2255:2263	arg1	transport					2366:2374	the transport	2362:2374	the transport of cytokines	2362:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	9	104	theme	open-source	1959:1969	arg1	www.febio.org					1987:1999	www.febio.org	1987:1999	www.febio.org	1987:1999	This implementation is released in the open-source program FEBio ( www.febio.org ).
24558059	9	104	theme	open-source	1959:1969	arg1	FEBio					1979:1983	the open-source program FEBio	1955:1983	the open-source program FEBio ( www.febio.org )	1955:2001	This implementation is released in the open-source program FEBio ( www.febio.org ).
24558059	10	105	from	influence	2442:2450	arg1	transport					2427:2435	transport	2427:2435	transport	2427:2435	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	105	from	influence	2442:2450	arg1	evolution					2178:2186	the evolution	2174:2186	the evolution of inhomogeneous tissue composition and mechanical properties	2174:2248	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	105	from	influence	2442:2450	arg1	kinetics					2474:2481	binding kinetics	2466:2481	binding kinetics	2466:2481	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	105	from	influence	2442:2450	arg1	transport					2366:2374	the transport	2362:2374	the transport of cytokines	2362:2387	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	106	theme	framework	2029:2037	arg1	beneficial					2059:2068	beneficial	2059:2068	beneficial	2059:2068	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	10	106	theme	framework	2029:2037	arg1	availability					2008:2019	The availability	2004:2019	The availability of this framework	2004:2037	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	2	107	theme	reactions	402:410	arg1	products					381:388	products	381:388	products	381:388	In many biological applications, the reactants and products of chemical reactions may be electrically charged, and these charge effects may produce driving forces and constraints that significantly influence outcomes.
24558059	2	107	theme	reactions	402:410	arg1	reactants					367:375	reactants	367:375	reactants	367:375	In many biological applications, the reactants and products of chemical reactions may be electrically charged, and these charge effects may produce driving forces and constraints that significantly influence outcomes.
24558059	0	108	theme	chemical	26:33	arg1	reactions					35:43	chemical reactions	26:43	chemical reactions	26:43	Computational modeling of chemical reactions and interstitial growth and remodeling involving charged solutes and solid-bound molecules.
24558059	2	109	theme	charge	451:456	arg1	effects					458:464	these charge effects	445:464	these charge effects	445:464	In many biological applications, the reactants and products of chemical reactions may be electrically charged, and these charge effects may produce driving forces and constraints that significantly influence outcomes.
24558059	3	110	theme	charged	697:703	arg1	mechanics					798:806	coupling mechanics	789:806	coupling mechanics with chemistry	789:821	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	3	110	theme	charged	697:703	arg1	solutes					705:711	charged solutes	697:711	charged solutes	697:711	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	7	111	theme	element	1653:1659	arg1	problems					1674:1681	Several finite element verification problems	1638:1681	Several finite element verification problems	1638:1681	Several finite element verification problems are shown to agree with closed-form analytical solutions.
24558059	0	112	theme	growth	62:67	arg1	modeling					14:21	Computational modeling	0:21	Computational modeling of chemical reactions and interstitial growth and remodeling	0:82	Computational modeling of chemical reactions and interstitial growth and remodeling involving charged solutes and solid-bound molecules.
24558059	7	113	theme	Several	1638:1644	arg1	problems					1674:1681	Several finite element verification problems	1638:1681	Several finite element verification problems	1638:1681	Several finite element verification problems are shown to agree with closed-form analytical solutions.
24558059	10	114	with	evolution	2255:2263	arg1	growth					2294:2299	growth	2294:2299	growth	2294:2299	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	4	115	theme	charged	1065:1071	arg1	species					1083:1089	charged molecular species	1065:1089	charged molecular species fixed to the solid matrix	1065:1115	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	3	116	theme	porous	759:764	arg1	matrix					781:786	a deformable porous hydrated solid matrix	746:786	a deformable porous hydrated solid matrix	746:786	In this study, a novel formulation and computational implementation are presented for modeling chemical reactions in biological tissues that involve charged solutes and solid-bound molecules within a deformable porous hydrated solid matrix, coupling mechanics with chemistry while accounting for electric charges.
24558059	4	117	theme	solid-bound	891:901	arg1	molecules					903:911	solid-bound molecules	891:911	solid-bound molecules	891:911	The deposition or removal of solid-bound molecules contributes to the growth and remodeling of the solid matrix; in particular, volumetric growth may be driven by Donnan osmotic swelling, resulting from charged molecular species fixed to the solid matrix.
24558059	5	118	with	chemistry	1251:1259	arg1	mechanics					1266:1274	mechanics	1266:1274	mechanics	1266:1274	This formulation incorporates the state of strain as a state variable in the production rate of chemical reactions, explicitly tying chemistry with mechanics for the purpose of modeling mechanobiology.
24558059	8	119	theme	tissue	1798:1803	arg1	growth					1805:1810	tissue growth	1798:1810	tissue growth	1798:1810	An illustrative tissue engineering analysis demonstrates tissue growth and swelling resulting from the deposition of chondroitin sulfate, a charged solid-bound molecular species.
24558059	5	120	theme	chemical	1214:1221	arg1	reactions					1223:1231	chemical reactions	1214:1231	chemical reactions	1214:1231	This formulation incorporates the state of strain as a state variable in the production rate of chemical reactions, explicitly tying chemistry with mechanics for the purpose of modeling mechanobiology.
24558059	2	121	from	charged	432:438	arg1	applications					349:360	many biological applications	333:360	many biological applications	333:360	In many biological applications, the reactants and products of chemical reactions may be electrically charged, and these charge effects may produce driving forces and constraints that significantly influence outcomes.
24558059	10	122	theme	tissue	2084:2089	arg1	systems					2111:2117	tissue engineering culture systems	2084:2117	tissue engineering culture systems	2084:2117	The availability of this framework may be particularly beneficial to optimizing tissue engineering culture systems by examining the influence of nutrient availability on the evolution of inhomogeneous tissue composition and mechanical properties, the evolution of construct dimensions with growth, the influence of solute and solid matrix electric charge on the transport of cytokines, the influence of binding kinetics on transport, the influence of loading on binding kinetics, and the differential growth response to dynamically loaded versus free-swelling culture conditions.
24558059	5	123	theme	modeling	1295:1302	arg1	mechanobiology					1304:1317	modeling mechanobiology	1295:1317	modeling mechanobiology	1295:1317	This formulation incorporates the state of strain as a state variable in the production rate of chemical reactions, explicitly tying chemistry with mechanics for the purpose of modeling mechanobiology.
24507281	3	0	attach	derived	699:705	arg2	activity					690:697	the predicted maximum activity	668:697	the predicted maximum activity	668:697	Under the optimized conditions, inulinase activity (21.058 U/gds) reached the predicted maximum activity derived from the taguchi methodology, which increased about 4.79-folds the initial production medium.
24507281	3	0	attach	derived	699:705	arg1	methodology					724:734	the taguchi methodology	712:734	the taguchi methodology	712:734	Under the optimized conditions, inulinase activity (21.058 U/gds) reached the predicted maximum activity derived from the taguchi methodology, which increased about 4.79-folds the initial production medium.
24507281	3	1	theme	maximum	682:688	arg1	activity					690:697	the predicted maximum activity	668:697	the predicted maximum activity	668:697	Under the optimized conditions, inulinase activity (21.058 U/gds) reached the predicted maximum activity derived from the taguchi methodology, which increased about 4.79-folds the initial production medium.
24507281	4	2	theme	inulin	845:850	arg1	hydrolysis					852:861	inulin hydrolysis	845:861	inulin hydrolysis	845:861	Fructose was produced, as an end product of inulin hydrolysis proving that the enzyme produced was exoinulinase.
24507281	5	3	theme	economic	1082:1089	arg1	inulin					1069:1074	inulin	1069:1074	inulin	1069:1074	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	5	3	theme	economic	1082:1089	arg1	source					1091:1096	an economic source	1079:1096	an economic source	1079:1096	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	2	4	theme	array	579:583	arg1	designs					585:591	Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs	528:591	Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs	528:591	Sequential optimization strategy, based on statistical experimental designs was employed to optimize the composition of the medium, including Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs.
24507281	2	5	theme	orthogonal	568:577	arg1	designs					585:591	Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs	528:591	Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs	528:591	Sequential optimization strategy, based on statistical experimental designs was employed to optimize the composition of the medium, including Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs.
24507281	0	6	from	substrates	51:60	arg1	Optimization					0:11	Optimization	0:11	Optimization of inulinase production from low cost substrates using Plackett-Burman and Taguchi methods.	0:103	Optimization of inulinase production from low cost substrates using Plackett-Burman and Taguchi methods.
24507281	4	7	theme	hydrolysis	852:861	arg1	product					834:840	an end product	827:840	an end product of inulin hydrolysis proving that the enzyme produced was exoinulinase	827:911	Fructose was produced, as an end product of inulin hydrolysis proving that the enzyme produced was exoinulinase.
24507281	4	7	theme	hydrolysis	852:861	arg1	Fructose					801:808	Fructose	801:808	Fructose	801:808	Fructose was produced, as an end product of inulin hydrolysis proving that the enzyme produced was exoinulinase.
24507281	1	8	theme	inulinase	177:185	arg1	enzyme					187:192	inulinase enzyme	177:192	inulinase enzyme	177:192	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	2	9	dep	orthogonal	568:577	arg1	Taguchi					548:554	Taguchi	548:554	Taguchi	548:554	Sequential optimization strategy, based on statistical experimental designs was employed to optimize the composition of the medium, including Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs.
24507281	2	9	dep	orthogonal	568:577	arg1	L9					559:560	L9 3(4)	559:565	L9 3(4)	559:565	Sequential optimization strategy, based on statistical experimental designs was employed to optimize the composition of the medium, including Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs.
24507281	2	9	dep	orthogonal	568:577	arg1	Plackett-Burman					528:542	Plackett-Burman	528:542	Plackett-Burman	528:542	Sequential optimization strategy, based on statistical experimental designs was employed to optimize the composition of the medium, including Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs.
24507281	5	10	theme	new	962:964	arg1	marine-derived					918:931	marine-derived	918:931	marine-derived	918:931	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	5	10	theme	new	962:964	arg1	candidate					976:984	a new potential candidate	960:984	a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source	960:1096	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	1	11	theme	enzyme	187:192	arg1	production					163:172	the production	159:172	the production of inulinase enzyme from low cost substrates	159:217	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	1	12	theme	solid	369:373	arg1	substrate					375:383	a solid substrate	367:383	a solid substrate	367:383	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	1	12	theme	solid	369:373	arg1	artichoke					347:355	artichoke	347:355	artichoke	347:355	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	1	13	theme	them	264:267	arg1	them					264:267	them	264:267	them	264:267	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	1	13	theme	them	264:267	arg1	one					257:259	one	257:259	one	257:259	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	4	14	theme	end	830:832	arg1	product					834:840	an end product	827:840	an end product of inulin hydrolysis proving that the enzyme produced was exoinulinase	827:911	Fructose was produced, as an end product of inulin hydrolysis proving that the enzyme produced was exoinulinase.
24507281	4	14	theme	end	830:832	arg1	Fructose					801:808	Fructose	801:808	Fructose	801:808	Fructose was produced, as an end product of inulin hydrolysis proving that the enzyme produced was exoinulinase.
24507281	1	15	theme	low	199:201	arg1	substrates					208:217	low cost substrates	199:217	low cost substrates	199:217	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	0	16	theme	production	26:35	arg1	Optimization					0:11	Optimization	0:11	Optimization of inulinase production from low cost substrates using Plackett-Burman and Taguchi methods.	0:103	Optimization of inulinase production from low cost substrates using Plackett-Burman and Taguchi methods.
24507281	1	17	dep	artichoke	347:355	arg1	leaves					357:362	leaves	357:362	leaves	357:362	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	0	18	dep	Plackett-Burman	68:82	arg1	methods					96:102	methods	96:102	methods	96:102	Optimization of inulinase production from low cost substrates using Plackett-Burman and Taguchi methods.
24507281	1	19	theme	cost	203:206	arg1	substrates					208:217	low cost substrates	199:217	low cost substrates	199:217	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	0	20	theme	inulinase	16:24	arg1	production					26:35	inulinase production	16:35	inulinase production	16:35	Optimization of inulinase production from low cost substrates using Plackett-Burman and Taguchi methods.
24507281	3	21	theme	taguchi	716:722	arg1	methodology					724:734	the taguchi methodology	712:734	the taguchi methodology	712:734	Under the optimized conditions, inulinase activity (21.058 U/gds) reached the predicted maximum activity derived from the taguchi methodology, which increased about 4.79-folds the initial production medium.
24507281	3	22	theme	inulinase	626:634	arg1	activity					636:643	inulinase activity	626:643	inulinase activity (21.058 U/gds)	626:658	Under the optimized conditions, inulinase activity (21.058 U/gds) reached the predicted maximum activity derived from the taguchi methodology, which increased about 4.79-folds the initial production medium.
24507281	3	22	theme	inulinase	626:634	arg1	U/gds					653:657	21.058 U/gds	646:657	21.058 U/gds	646:657	Under the optimized conditions, inulinase activity (21.058 U/gds) reached the predicted maximum activity derived from the taguchi methodology, which increased about 4.79-folds the initial production medium.
24507281	1	23	theme	marine-derived	110:123	arg1	isolates					132:139	Four marine-derived fungal isolates	105:139	Four marine-derived fungal isolates	105:139	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	0	24	theme	low	42:44	arg1	substrates					51:60	low cost substrates	42:60	low cost substrates using Plackett-Burman and Taguchi methods	42:102	Optimization of inulinase production from low cost substrates using Plackett-Burman and Taguchi methods.
24507281	5	25	theme	industrial	990:999	arg1	production					1011:1020	industrial enzymatic production	990:1020	industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source	990:1096	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	5	26	theme	enzymatic	1001:1009	arg1	production					1011:1020	industrial enzymatic production	990:1020	industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source	990:1096	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	2	27	theme	optimization	397:408	arg1	strategy					410:417	Sequential optimization strategy	386:417	Sequential optimization strategy	386:417	Sequential optimization strategy, based on statistical experimental designs was employed to optimize the composition of the medium, including Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs.
24507281	2	28	theme	experimental	441:452	arg1	designs					454:460	statistical experimental designs	429:460	statistical experimental designs	429:460	Sequential optimization strategy, based on statistical experimental designs was employed to optimize the composition of the medium, including Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs.
24507281	1	29	theme	solid	225:229	arg1	SSF					251:253	SSF	251:253	SSF	251:253	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	1	29	theme	solid	225:229	arg1	fermentation					237:248	solid state fermentation	225:248	solid state fermentation (SSF)	225:254	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	5	30	from	substrate	1048:1056	arg1	production					1011:1020	industrial enzymatic production	990:1020	industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source	990:1096	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	1	31	theme	fungal	125:130	arg1	isolates					132:139	Four marine-derived fungal isolates	105:139	Four marine-derived fungal isolates	105:139	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	0	32	theme	cost	46:49	arg1	substrates					51:60	low cost substrates	42:60	low cost substrates using Plackett-Burman and Taguchi methods	42:102	Optimization of inulinase production from low cost substrates using Plackett-Burman and Taguchi methods.
24507281	3	33	theme	predicted	672:680	arg1	activity					690:697	the predicted maximum activity	668:697	the predicted maximum activity	668:697	Under the optimized conditions, inulinase activity (21.058 U/gds) reached the predicted maximum activity derived from the taguchi methodology, which increased about 4.79-folds the initial production medium.
24507281	5	34	theme	fructose	1025:1032	arg1	production					1011:1020	industrial enzymatic production	990:1020	industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source	990:1096	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	1	35	theme	highest	314:320	arg1	activity					332:339	the highest inulinase activity	310:339	the highest inulinase activity	310:339	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	5	36	theme	low	1039:1041	arg1	substrate					1048:1056	low cost substrate	1039:1056	low cost substrate containing inulin as an economic source	1039:1096	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	5	37	theme	potential	966:974	arg1	marine-derived					918:931	marine-derived	918:931	marine-derived	918:931	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	5	37	theme	potential	966:974	arg1	candidate					976:984	a new potential candidate	960:984	a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source	960:1096	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	1	38	from	substrates	208:217	arg1	production					163:172	the production	159:172	the production of inulinase enzyme from low cost substrates	159:217	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	1	39	theme	state	231:235	arg1	SSF					251:253	SSF	251:253	SSF	251:253	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	1	39	theme	state	231:235	arg1	fermentation					237:248	solid state fermentation	225:248	solid state fermentation (SSF)	225:254	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	5	40	theme	cost	1043:1046	arg1	substrate					1048:1056	low cost substrate	1039:1056	low cost substrate containing inulin as an economic source	1039:1096	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	3	41	theme	optimized	604:612	arg1	conditions					614:623	the optimized conditions	600:623	the optimized conditions	600:623	Under the optimized conditions, inulinase activity (21.058 U/gds) reached the predicted maximum activity derived from the taguchi methodology, which increased about 4.79-folds the initial production medium.
24507281	2	42	theme	medium	510:515	arg1	composition					491:501	the composition	487:501	the composition	487:501	Sequential optimization strategy, based on statistical experimental designs was employed to optimize the composition of the medium, including Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs.
24507281	5	43	contain	containing	1058:1067	arg2	inulin					1069:1074	inulin	1069:1074	inulin	1069:1074	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	5	43	contain	containing	1058:1067	arg1	substrate					1048:1056	low cost substrate	1039:1056	low cost substrate containing inulin as an economic source	1039:1096	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	5	43	contain	containing	1058:1067	arg2	source					1091:1096	an economic source	1079:1096	an economic source	1079:1096	The marine-derived A. terreus is suggested as a new potential candidate for industrial enzymatic production of fructose from low cost substrate containing inulin as an economic source.
24507281	2	44	theme	Sequential	386:395	arg1	strategy					410:417	Sequential optimization strategy	386:417	Sequential optimization strategy	386:417	Sequential optimization strategy, based on statistical experimental designs was employed to optimize the composition of the medium, including Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs.
24507281	3	45	theme	initial	774:780	arg1	medium					793:798	4.79-folds the initial production medium	759:798	4.79-folds the initial production medium	759:798	Under the optimized conditions, inulinase activity (21.058 U/gds) reached the predicted maximum activity derived from the taguchi methodology, which increased about 4.79-folds the initial production medium.
24507281	1	46	link	marine-derived	110:123	arg1	isolates					132:139	Four marine-derived fungal isolates	105:139	Four marine-derived fungal isolates	105:139	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	1	47	theme	inulinase	322:330	arg1	activity					332:339	the highest inulinase activity	310:339	the highest inulinase activity	310:339	Four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (SSF), one of them identified as Aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate.
24507281	2	48	theme	statistical	429:439	arg1	designs					454:460	statistical experimental designs	429:460	statistical experimental designs	429:460	Sequential optimization strategy, based on statistical experimental designs was employed to optimize the composition of the medium, including Plackett-Burman and Taguchi's (L9 3(4)) orthogonal array designs.
24507281	3	49	theme	production	782:791	arg1	medium					793:798	4.79-folds the initial production medium	759:798	4.79-folds the initial production medium	759:798	Under the optimized conditions, inulinase activity (21.058 U/gds) reached the predicted maximum activity derived from the taguchi methodology, which increased about 4.79-folds the initial production medium.
27653554	2	0	theme	different	495:503	arg1	percentages					505:515	different percentages	495:515	different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material	495:565	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	7	1	theme	HPC	1279:1281	arg1	HPC					1279:1281	HPC	1279:1281	HPC	1279:1281	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	7	1	theme	HPC	1279:1281	arg1	%					1274:1274	0.65%	1270:1274	0.65% of HPC	1270:1281	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	2	2	theme	coating	550:556	arg1	material					558:565	coating material	550:565	coating material	550:565	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	6	3	theme	better	1169:1174	arg1	performance					1176:1186	better performance	1169:1186	better performance	1169:1186	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	6	4	theme	Eudragit	1193:1200	arg1	D-55					1206:1209	Eudragit L30 D-55	1193:1209	Eudragit L30 D-55	1193:1209	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	4	5	theme	swelling	690:697	arg1	process					699:705	the swelling process	686:705	the swelling process	686:705	The rate and the entity of the swelling process were affected by the polymeric composition: increasing the HPC concentration, the structure of the pellets became more compact and slowed down the penetration of fluids.
27653554	7	6	theme	best	1327:1330	arg1	formulation					1332:1342	the best formulation	1323:1342	the best formulation	1323:1342	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	5	7	from	quantity	972:979	arg1	system					995:1000	the system	991:1000	the system	991:1000	Coated alginate-HPC formulations were able to control the drug release at neutral pH: a higher quantity of HPC in the system determined a slower release of the drug.
27653554	2	8	theme	ionotropic	400:409	arg1	gelation					411:418	ionotropic gelation	400:418	ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material	400:565	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	6	9	from	release	1125:1131	arg1	environment					1143:1153	acidic environment	1136:1153	acidic environment	1136:1153	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	6	10	theme	coating	1085:1091	arg1	level					1093:1097	the coating level	1081:1097	the coating level applied	1081:1105	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	2	11	dep	gelation	411:418	arg1	applying					486:493	applying	486:493	applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material	486:565	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	2	11	dep	gelation	411:418	arg1	using					420:424	using	420:424	using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt)	420:480	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	6	12	theme	drug	1120:1123	arg1	release					1125:1131	the drug release	1116:1131	the drug release in acidic environment	1116:1153	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	7	13	theme	%	1301:1301	arg1	L100					1306:1309	20% EU L100	1299:1309	20% EU L100	1299:1309	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	5	14	theme	alginate-HPC	884:895	arg1	formulations					897:908	Coated alginate-HPC formulations	877:908	Coated alginate-HPC formulations	877:908	Coated alginate-HPC formulations were able to control the drug release at neutral pH: a higher quantity of HPC in the system determined a slower release of the drug.
27653554	2	15	theme	alginate	324:331	arg1	pellets					375:381	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets	271:381	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets	271:381	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	7	16	theme	acidic	1379:1384	arg1	environment					1386:1396	acidic environment	1379:1396	acidic environment	1379:1396	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	3	17	from	regular	594:600	arg1	shape					605:609	shape	605:609	shape	605:609	The uncoated pellets were regular in shape and had mean diameter between 1490 and 1570 μm.
27653554	7	18	theme	EU	1303:1304	arg1	L100					1306:1309	20% EU L100	1299:1309	20% EU L100	1299:1309	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	4	19	theme	HPC	766:768	arg1	concentration					770:782	the HPC concentration	762:782	the HPC concentration	762:782	The rate and the entity of the swelling process were affected by the polymeric composition: increasing the HPC concentration, the structure of the pellets became more compact and slowed down the penetration of fluids.
27653554	2	20	theme	D-55-coated	312:322	arg1	alginate					324:331	Eudragit L30 D-55-coated alginate	299:331	Eudragit L30 D-55-coated alginate	299:331	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	2	20	theme	D-55-coated	312:322	arg1	HPC					370:372	HPC	370:372	HPC	370:372	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	4	21	theme	fluids	869:874	arg1	penetration					854:864	the penetration	850:864	the penetration of fluids	850:874	The rate and the entity of the swelling process were affected by the polymeric composition: increasing the HPC concentration, the structure of the pellets became more compact and slowed down the penetration of fluids.
27653554	6	22	theme	coating	1224:1230	arg1	level					1232:1236	the best coating level	1215:1236	the best coating level	1215:1236	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	6	22	theme	coating	1224:1230	arg1	%					1244:1244	20%	1242:1244	20%	1242:1244	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	6	23	theme	acidic	1136:1141	arg1	environment					1143:1153	acidic environment	1136:1153	acidic environment	1136:1153	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	2	24	theme	L30	308:310	arg1	alginate					324:331	Eudragit L30 D-55-coated alginate	299:331	Eudragit L30 D-55-coated alginate	299:331	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	2	24	theme	L30	308:310	arg1	HPC					370:372	HPC	370:372	HPC	370:372	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	7	25	theme	20	1299:1300	arg1	%					1301:1301	%	1301:1301	%	1301:1301	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	2	26	theme	HPC	446:448	arg1	concentrations					428:441	3 concentrations	426:441	3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt)	426:480	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	1	27	contain	containing	171:180	arg2	ranolazine					182:191	ranolazine	182:191	ranolazine	182:191	The formulation and the coating composition of biopolymeric pellets containing ranolazine were studied to improve their technological and biopharmaceutical properties.
27653554	1	27	contain	containing	171:180	arg1	pellets					163:169	biopolymeric pellets	150:169	biopolymeric pellets containing ranolazine	150:191	The formulation and the coating composition of biopolymeric pellets containing ranolazine were studied to improve their technological and biopharmaceutical properties.
27653554	4	28	theme	pellets	806:812	arg1	structure					789:797	the structure	785:797	the structure of the pellets	785:812	The rate and the entity of the swelling process were affected by the polymeric composition: increasing the HPC concentration, the structure of the pellets became more compact and slowed down the penetration of fluids.
27653554	2	29	theme	material	558:565	arg1	percentages					505:515	different percentages	495:515	different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material	495:565	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	7	30	theme	able	1345:1348	arg1	formulation					1332:1342	the best formulation	1323:1342	the best formulation	1323:1342	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	1	31	theme	technological	223:235	arg1	properties					259:268	their technological and biopharmaceutical properties	217:268	their technological and biopharmaceutical properties	217:268	The formulation and the coating composition of biopolymeric pellets containing ranolazine were studied to improve their technological and biopharmaceutical properties.
27653554	0	32	dep	Alginate	27:34	arg1	Pellets					72:78	Pellets	72:78	Pellets	72:78	Formulation and Coating of Alginate and Alginate-Hydroxypropylcellulose Pellets Containing Ranolazine.
27653554	5	33	theme	drug	935:938	arg1	release					940:946	the drug release	931:946	the drug release	931:946	Coated alginate-HPC formulations were able to control the drug release at neutral pH: a higher quantity of HPC in the system determined a slower release of the drug.
27653554	5	34	theme	Coated	877:882	arg1	formulations					897:908	Coated alginate-HPC formulations	877:908	Coated alginate-HPC formulations	877:908	Coated alginate-HPC formulations were able to control the drug release at neutral pH: a higher quantity of HPC in the system determined a slower release of the drug.
27653554	3	35	dep	1490	641:644	arg1	μm					655:656	μm	655:656	μm	655:656	The uncoated pellets were regular in shape and had mean diameter between 1490 and 1570 μm.
27653554	4	36	theme	process	699:705	arg1	rate					663:666	The rate	659:666	The rate	659:666	The rate and the entity of the swelling process were affected by the polymeric composition: increasing the HPC concentration, the structure of the pellets became more compact and slowed down the penetration of fluids.
27653554	4	36	theme	process	699:705	arg1	entity					676:681	the entity	672:681	the entity	672:681	The rate and the entity of the swelling process were affected by the polymeric composition: increasing the HPC concentration, the structure of the pellets became more compact and slowed down the penetration of fluids.
27653554	6	37	theme	polymer	1069:1075	arg1	level					1093:1097	the coating level	1081:1097	the coating level applied	1081:1105	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	6	37	theme	polymer	1069:1075	arg1	nature					1047:1052	The nature	1043:1052	The nature of the coating polymer	1043:1075	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	1	38	theme	coating	127:133	arg1	composition					135:145	the coating composition	123:145	the coating composition of biopolymeric pellets containing ranolazine	123:191	The formulation and the coating composition of biopolymeric pellets containing ranolazine were studied to improve their technological and biopharmaceutical properties.
27653554	1	39	theme	biopharmaceutical	241:257	arg1	properties					259:268	their technological and biopharmaceutical properties	217:268	their technological and biopharmaceutical properties	217:268	The formulation and the coating composition of biopolymeric pellets containing ranolazine were studied to improve their technological and biopharmaceutical properties.
27653554	7	40	theme	drug	1363:1366	arg1	release					1368:1374	the drug release	1359:1374	the drug release in acidic environment	1359:1396	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	7	41	from	release	1368:1374	arg1	environment					1386:1396	acidic environment	1379:1396	acidic environment	1379:1396	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	2	42	dep	%	455:455	arg1	wt/wt					475:479	wt/wt	475:479	wt/wt	475:479	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	3	43	contain	had	615:617	arg1	regular					594:600	regular	594:600	regular	594:600	The uncoated pellets were regular in shape and had mean diameter between 1490 and 1570 μm.
27653554	3	43	contain	had	615:617	arg2	diameter					624:631	mean diameter	619:631	mean diameter between 1490 and 1570 μm	619:656	The uncoated pellets were regular in shape and had mean diameter between 1490 and 1570 μm.
27653554	3	43	contain	had	615:617	arg1	pellets					581:587	The uncoated pellets	568:587	The uncoated pellets	568:587	The uncoated pellets were regular in shape and had mean diameter between 1490 and 1570 μm.
27653554	0	44	theme	Alginate	27:34	arg1	Coating					16:22	Coating	16:22	Coating	16:22	Formulation and Coating of Alginate and Alginate-Hydroxypropylcellulose Pellets Containing Ranolazine.
27653554	0	44	theme	Alginate	27:34	arg1	Formulation					0:10	Formulation	0:10	Formulation	0:10	Formulation and Coating of Alginate and Alginate-Hydroxypropylcellulose Pellets Containing Ranolazine.
27653554	5	45	theme	drug	1037:1040	arg1	release					1022:1028	a slower release	1013:1028	a slower release of the drug	1013:1040	Coated alginate-HPC formulations were able to control the drug release at neutral pH: a higher quantity of HPC in the system determined a slower release of the drug.
27653554	6	46	theme	L30	1202:1204	arg1	D-55					1206:1209	Eudragit L30 D-55	1193:1209	Eudragit L30 D-55	1193:1209	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	5	47	theme	HPC	984:986	arg1	quantity					972:979	a higher quantity	963:979	a higher quantity of HPC in the system	963:1000	Coated alginate-HPC formulations were able to control the drug release at neutral pH: a higher quantity of HPC in the system determined a slower release of the drug.
27653554	4	48	theme	polymeric	728:736	arg1	composition					738:748	the polymeric composition	724:748	the polymeric composition	724:748	The rate and the entity of the swelling process were affected by the polymeric composition: increasing the HPC concentration, the structure of the pellets became more compact and slowed down the penetration of fluids.
27653554	6	49	theme	best	1219:1222	arg1	level					1232:1236	the best coating level	1215:1236	the best coating level	1215:1236	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	6	49	theme	best	1219:1222	arg1	%					1244:1244	20%	1242:1244	20%	1242:1244	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	2	50	theme	%	538:538	arg1	wt/wt					540:544	30% wt/wt	536:544	30% wt/wt	536:544	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	2	51	theme	L100	280:283	arg1	pellets					375:381	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets	271:381	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets	271:381	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	0	52	theme	Alginate-Hydroxypropylcellulose	40:70	arg1	Coating					16:22	Coating	16:22	Coating	16:22	Formulation and Coating of Alginate and Alginate-Hydroxypropylcellulose Pellets Containing Ranolazine.
27653554	0	52	theme	Alginate-Hydroxypropylcellulose	40:70	arg1	Formulation					0:10	Formulation	0:10	Formulation	0:10	Formulation and Coating of Alginate and Alginate-Hydroxypropylcellulose Pellets Containing Ranolazine.
27653554	6	53	theme	coating	1061:1067	arg1	polymer					1069:1075	the coating polymer	1057:1075	the coating polymer	1057:1075	The nature of the coating polymer and the coating level applied affected the drug release in acidic environment: EU L100 gave better performance than Eudragit L30 D-55 and the best coating level was 20%.
27653554	2	54	theme	30	536:537	arg1	%					538:538	%	538:538	%	538:538	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	5	55	theme	neutral	951:957	arg1	pH					959:960	neutral pH	951:960	neutral pH	951:960	Coated alginate-HPC formulations were able to control the drug release at neutral pH: a higher quantity of HPC in the system determined a slower release of the drug.
27653554	2	56	theme	Eudragit	271:278	arg1	L100					280:283	Eudragit L100	271:283	Eudragit L100 (EU L100)	271:293	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	2	56	theme	Eudragit	271:278	arg1	L100					289:292	EU L100	286:292	EU L100	286:292	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	3	57	theme	mean	619:622	arg1	diameter					624:631	mean diameter	619:631	mean diameter between 1490 and 1570 μm	619:656	The uncoated pellets were regular in shape and had mean diameter between 1490 and 1570 μm.
27653554	2	58	theme	Eudragit	299:306	arg1	alginate					324:331	Eudragit L30 D-55-coated alginate	299:331	Eudragit L30 D-55-coated alginate	299:331	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	2	58	theme	Eudragit	299:306	arg1	HPC					370:372	HPC	370:372	HPC	370:372	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	1	59	theme	biopolymeric	150:161	arg1	pellets					163:169	biopolymeric pellets	150:169	biopolymeric pellets containing ranolazine	150:191	The formulation and the coating composition of biopolymeric pellets containing ranolazine were studied to improve their technological and biopharmaceutical properties.
27653554	2	60	dep	percentages	505:515	arg1	%					519:519	5%	518:519	5%	518:519	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	2	60	dep	percentages	505:515	arg1	%					524:524	10%	522:524	10%	522:524	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	2	60	dep	percentages	505:515	arg1	%					529:529	20%	527:529	20%	527:529	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	2	60	dep	percentages	505:515	arg1	wt/wt					540:544	30% wt/wt	536:544	30% wt/wt	536:544	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	3	61	theme	uncoated	572:579	arg1	regular					594:600	regular	594:600	regular	594:600	The uncoated pellets were regular in shape and had mean diameter between 1490 and 1570 μm.
27653554	3	61	theme	uncoated	572:579	arg1	pellets					581:587	The uncoated pellets	568:587	The uncoated pellets	568:587	The uncoated pellets were regular in shape and had mean diameter between 1490 and 1570 μm.
27653554	1	62	theme	pellets	163:169	arg1	composition					135:145	the coating composition	123:145	the coating composition of biopolymeric pellets containing ranolazine	123:191	The formulation and the coating composition of biopolymeric pellets containing ranolazine were studied to improve their technological and biopharmaceutical properties.
27653554	1	62	theme	pellets	163:169	arg1	formulation					107:117	The formulation	103:117	The formulation	103:117	The formulation and the coating composition of biopolymeric pellets containing ranolazine were studied to improve their technological and biopharmaceutical properties.
27653554	3	63	from	shape	605:609	arg1	regular					594:600	regular	594:600	regular	594:600	The uncoated pellets were regular in shape and had mean diameter between 1490 and 1570 μm.
27653554	3	63	from	shape	605:609	arg1	pellets					581:587	The uncoated pellets	568:587	The uncoated pellets	568:587	The uncoated pellets were regular in shape and had mean diameter between 1490 and 1570 μm.
27653554	5	64	theme	higher	965:970	arg1	quantity					972:979	a higher quantity	963:979	a higher quantity of HPC in the system	963:1000	Coated alginate-HPC formulations were able to control the drug release at neutral pH: a higher quantity of HPC in the system determined a slower release of the drug.
27653554	7	65	dep	pellets	1251:1257	arg1	containing					1259:1268	containing	1259:1268	containing 0.65% of HPC	1259:1281	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	7	65	dep	pellets	1251:1257	arg1	coated					1287:1292	coated	1287:1292	coated with 20% EU L100	1287:1309	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	7	66	dep	able	1345:1348	arg1	control					1405:1411	control	1405:1411	to control it at pH 6.8	1402:1424	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	7	66	dep	able	1345:1348	arg1	limit					1353:1357	limit	1353:1357	to limit the drug release in acidic environment	1350:1396	The pellets containing 0.65% of HPC and coated with 20% EU L100 represented the best formulation, able to limit the drug release in acidic environment and to control it at pH 6.8.
27653554	5	67	theme	slower	1015:1020	arg1	release					1022:1028	a slower release	1013:1028	a slower release of the drug	1013:1040	Coated alginate-HPC formulations were able to control the drug release at neutral pH: a higher quantity of HPC in the system determined a slower release of the drug.
27653554	2	68	dep	HPC	446:448	arg1	%					455:455	0.50%	451:455	0.50%	451:455	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	2	68	dep	HPC	446:448	arg1	%					462:462	0.65%	458:462	0.65%	458:462	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
27653554	2	68	dep	HPC	446:448	arg1	%					473:473	1.00%	469:473	1.00%	469:473	Eudragit L100 (EU L100) and Eudragit L30 D-55-coated alginate and alginate-hydroxypropylcellulose (HPC) pellets were prepared by ionotropic gelation using 3 concentrations of HPC (0.50%, 0.65%, and 1.00% wt/wt) and applying different percentages (5%, 10%, 20%, and 30% wt/wt) of coating material.
28386058	5	0	theme	dry	716:718	arg1	ratio					729:733	dry weight % ratio	716:733	dry weight % ratio	716:733	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	0	theme	dry	716:718	arg1	60/40					709:713	60/40	709:713	60/40	709:713	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	1	theme	weight	720:725	arg1	ratio					729:733	dry weight % ratio	716:733	dry weight % ratio	716:733	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	1	theme	weight	720:725	arg1	60/40					709:713	60/40	709:713	60/40	709:713	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	3	2	with	NFC	508:510	arg1	acid					548:551	hyaluronic acid	537:551	hyaluronic acid (NFC/HA)	537:560	Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA).
28386058	3	2	with	NFC	508:510	arg1	NFC/HA					554:559	NFC/HA	554:559	NFC/HA	554:559	Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA).
28386058	3	2	with	NFC	508:510	arg1	NFC/A					527:531	NFC/A	527:531	NFC/A	527:531	Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA).
28386058	3	2	with	NFC	508:510	arg1	alginate					517:524	alginate	517:524	alginate (NFC/A)	517:532	Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA).
28386058	5	3	theme	collagen	848:855	arg1	type					857:860	collagen type II	848:863	collagen type II expression	848:874	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	4	theme	3D	932:933	arg1	NFC/A					947:951	3D -bioprinted NFC/A	932:951	3D -bioprinted NFC/A (60/40, dry weight % relation) constructs	932:993	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	5	theme	weight	965:970	arg1	relation					974:981	dry weight % relation	961:981	dry weight % relation	961:981	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	5	theme	weight	965:970	arg1	60/40					954:958	60/40	954:958	60/40	954:958	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	6	6	theme	high	1150:1153	arg1	densities					1160:1168	high cell densities	1150:1168	high cell densities	1150:1168	Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing.
28386058	7	7	theme	irradiated	1344:1353	arg1	chondrocytes					1355:1366	irradiated chondrocytes	1344:1366	irradiated chondrocytes	1344:1366	We conclude that NFC/A bioink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes.
28386058	2	8	link	human-derived	262:274	arg1	treatment					235:243	a prospective treatment	221:243	a prospective treatment of such lesions	221:259	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	8	link	human-derived	262:274	arg1	iPSCs					308:312	iPSCs	308:312	iPSCs	308:312	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	8	link	human-derived	262:274	arg1	cells					301:305	human-derived induced pluripotent stem cells	262:305	human-derived induced pluripotent stem cells (iPSCs)	262:313	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	0	9	theme	Nanocellulose/Alginate	69:90	arg1	Bioink					92:97	a Nanocellulose/Alginate Bioink	67:97	a Nanocellulose/Alginate Bioink	67:97	Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink.
28386058	5	10	with	tissue	836:841	arg1	expression					865:874	collagen type II expression	848:874	collagen type II expression	848:874	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	2	11	theme	stem	296:299	arg1	treatment					235:243	a prospective treatment	221:243	a prospective treatment of such lesions	221:259	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	11	theme	stem	296:299	arg1	iPSCs					308:312	iPSCs	308:312	iPSCs	308:312	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	11	theme	stem	296:299	arg1	cells					301:305	human-derived induced pluripotent stem cells	262:305	human-derived induced pluripotent stem cells (iPSCs)	262:313	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	6	12	theme	fluorescence	1096:1107	arg1	microscopy					1109:1118	2-photon fluorescence microscopy	1087:1118	2-photon fluorescence microscopy	1087:1118	Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing.
28386058	4	13	theme	Low	563:565	arg1	proliferation					567:579	Low proliferation	563:579	Low proliferation	563:579	Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA.
28386058	5	14	theme	NFC/A	702:706	arg1	constructs					736:745	the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs	684:745	the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs	684:745	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	0	15	from	Bioprinting	39:49	arg1	Bioink					92:97	a Nanocellulose/Alginate Bioink	67:97	a Nanocellulose/Alginate Bioink	67:97	Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink.
28386058	6	16	theme	good	1198:1201	arg1	survival					1203:1210	good survival	1198:1210	good survival after printing	1198:1225	Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing.
28386058	6	17	theme	marked	1008:1013	arg1	increase					1015:1022	a marked increase	1006:1022	a marked increase in cell number within the cartilaginous tissue	1006:1069	Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing.
28386058	2	18	theme	pluripotent	284:294	arg1	treatment					235:243	a prospective treatment	221:243	a prospective treatment of such lesions	221:259	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	18	theme	pluripotent	284:294	arg1	iPSCs					308:312	iPSCs	308:312	iPSCs	308:312	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	18	theme	pluripotent	284:294	arg1	cells					301:305	human-derived induced pluripotent stem cells	262:305	human-derived induced pluripotent stem cells (iPSCs)	262:313	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	1	19	theme	severe	171:176	arg1	problems					187:194	severe clinical problems	171:194	severe clinical problems in numerous patients	171:215	Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients.
28386058	5	20	theme	constructs	736:745	arg1	case					676:679	the case	672:679	the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs	672:745	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	21	theme	%	727:727	arg1	ratio					729:733	dry weight % ratio	716:733	dry weight % ratio	716:733	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	21	theme	%	727:727	arg1	60/40					709:713	60/40	709:713	60/40	709:713	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	7	22	theme	NFC/A	1245:1249	arg1	bioink					1251:1256	NFC/A bioink	1245:1256	NFC/A bioink	1245:1256	We conclude that NFC/A bioink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes.
28386058	2	23	theme	induced	276:282	arg1	treatment					235:243	a prospective treatment	221:243	a prospective treatment of such lesions	221:259	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	23	theme	induced	276:282	arg1	iPSCs					308:312	iPSCs	308:312	iPSCs	308:312	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	23	theme	induced	276:282	arg1	cells					301:305	human-derived induced pluripotent stem cells	262:305	human-derived induced pluripotent stem cells (iPSCs)	262:313	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	24	theme	nanofibrillated	376:390	arg1	bioink					418:423	a nanofibrillated cellulose (NFC) composite bioink	374:423	a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes	374:474	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	0	25	theme	Tissue	10:15	arg1	Engineering					17:27	Cartilage Tissue Engineering	0:27	Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink.	0:98	Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink.
28386058	6	26	theme	2-photon	1087:1094	arg1	microscopy					1109:1118	2-photon fluorescence microscopy	1087:1118	2-photon fluorescence microscopy	1087:1118	Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing.
28386058	0	27	theme	Cartilage	0:8	arg1	Engineering					17:27	Cartilage Tissue Engineering	0:27	Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink.	0:98	Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink.
28386058	5	28	located	observed	920:927	arg1	constructs					984:993	3D -bioprinted NFC/A (60/40, dry weight % relation) constructs	932:993	3D -bioprinted NFC/A (60/40, dry weight % relation) constructs	932:993	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	28	located	observed	920:927	arg2	tissue					836:841	hyaline-like cartilaginous tissue	809:841	hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression	809:914	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	29	theme	tumorigenic	888:898	arg1	expression					905:914	tumorigenic Oct4 expression	888:914	tumorigenic Oct4 expression	888:914	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	30	theme	hyaline-like	809:820	arg1	tissue					836:841	hyaline-like cartilaginous tissue	809:841	hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression	809:914	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	7	31	theme	cartilage	1303:1311	arg1	production					1313:1322	cartilage production	1303:1322	cartilage production in co-cultures with irradiated chondrocytes	1303:1366	We conclude that NFC/A bioink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes.
28386058	6	32	theme	cell	1155:1158	arg1	densities					1160:1168	high cell densities	1150:1168	high cell densities	1150:1168	Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing.
28386058	3	33	theme	hyaluronic	537:546	arg1	acid					548:551	hyaluronic acid	537:551	hyaluronic acid (NFC/HA)	537:560	Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA).
28386058	3	33	theme	hyaluronic	537:546	arg1	NFC/HA					554:559	NFC/HA	554:559	NFC/HA	554:559	Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA).
28386058	7	34	from	production	1313:1322	arg1	co-cultures					1327:1337	co-cultures	1327:1337	co-cultures with irradiated chondrocytes	1327:1366	We conclude that NFC/A bioink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes.
28386058	2	35	theme	composite	408:416	arg1	bioink					418:423	a nanofibrillated cellulose (NFC) composite bioink	374:423	a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes	374:474	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	6	36	from	increase	1015:1022	arg1	number					1032:1037	cell number	1027:1037	cell number within the cartilaginous tissue	1027:1069	Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing.
28386058	1	37	theme	Cartilage	100:108	arg1	lesions					110:116	Cartilage lesions	100:116	Cartilage lesions	100:116	Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients.
28386058	4	38	from	pluripotency	614:625	arg1	proliferation					567:579	Low proliferation	563:579	Low proliferation	563:579	Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA.
28386058	4	38	from	pluripotency	614:625	arg1	changes					596:602	phenotypic changes	585:602	phenotypic changes	585:602	Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA.
28386058	5	39	theme	cartilaginous	822:834	arg1	tissue					836:841	hyaline-like cartilaginous tissue	809:841	hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression	809:914	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	0	40	theme	3D	36:37	arg1	Bioprinting					39:49	the 3D Bioprinting	32:49	the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink	32:97	Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink.
28386058	6	41	theme	densities	1160:1168	arg1	importance					1136:1145	the importance	1132:1145	the importance of high cell densities in the pursuit of achieving good survival after printing	1132:1225	Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing.
28386058	2	42	theme	NFC	403:405	arg1	bioink					418:423	a nanofibrillated cellulose (NFC) composite bioink	374:423	a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes	374:474	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	43	theme	cellulose	392:400	arg1	bioink					418:423	a nanofibrillated cellulose (NFC) composite bioink	374:423	a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes	374:474	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	5	44	theme	dry	961:963	arg1	weight					965:970	dry weight	961:970	dry weight % relation	961:981	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	7	45	with	co-cultures	1327:1337	arg1	chondrocytes					1355:1366	irradiated chondrocytes	1344:1366	irradiated chondrocytes	1344:1366	We conclude that NFC/A bioink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes.
28386058	5	46	dep	NFC/A	947:951	arg1	relation					974:981	dry weight % relation	961:981	dry weight % relation	961:981	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	46	dep	NFC/A	947:951	arg1	60/40					954:958	60/40	954:958	60/40	954:958	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	2	47	theme	human	457:461	arg1	chondrocytes					463:474	irradiated human chondrocytes	446:474	irradiated human chondrocytes	446:474	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	5	48	dep	NFC/A	702:706	arg1	ratio					729:733	dry weight % ratio	716:733	dry weight % ratio	716:733	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	48	dep	NFC/A	702:706	arg1	60/40					709:713	60/40	709:713	60/40	709:713	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	2	49	theme	prospective	223:233	arg1	treatment					235:243	a prospective treatment	221:243	a prospective treatment of such lesions	221:259	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	49	theme	prospective	223:233	arg1	cells					301:305	human-derived induced pluripotent stem cells	262:305	human-derived induced pluripotent stem cells (iPSCs)	262:313	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	5	50	theme	type	857:860	arg1	expression					865:874	collagen type II expression	848:874	collagen type II expression	848:874	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	2	51	theme	irradiated	446:455	arg1	chondrocytes					463:474	irradiated human chondrocytes	446:474	irradiated human chondrocytes	446:474	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	5	52	theme	%	972:972	arg1	relation					974:981	dry weight % relation	961:981	dry weight % relation	961:981	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	5	52	theme	%	972:972	arg1	60/40					954:958	60/40	954:958	60/40	954:958	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	6	53	theme	cartilaginous	1050:1062	arg1	tissue					1064:1069	the cartilaginous tissue	1046:1069	the cartilaginous tissue	1046:1069	Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing.
28386058	5	54	theme	-bioprinted	935:945	arg1	NFC/A					947:951	3D -bioprinted NFC/A	932:951	3D -bioprinted NFC/A (60/40, dry weight % relation) constructs	932:993	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	2	55	theme	human-derived	262:274	arg1	treatment					235:243	a prospective treatment	221:243	a prospective treatment of such lesions	221:259	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	55	theme	human-derived	262:274	arg1	iPSCs					308:312	iPSCs	308:312	iPSCs	308:312	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	55	theme	human-derived	262:274	arg1	cells					301:305	human-derived induced pluripotent stem cells	262:305	human-derived induced pluripotent stem cells (iPSCs)	262:313	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	5	56	theme	Oct4	900:903	arg1	expression					905:914	tumorigenic Oct4 expression	888:914	tumorigenic Oct4 expression	888:914	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	4	57	theme	phenotypic	585:594	arg1	changes					596:602	phenotypic changes	585:602	phenotypic changes	585:602	Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA.
28386058	2	58	dep	cartilage	351:359	arg1	mimics					361:366	mimics	361:366	mimics	361:366	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	5	59	theme	NFC/A	947:951	arg1	constructs					984:993	3D -bioprinted NFC/A (60/40, dry weight % relation) constructs	932:993	3D -bioprinted NFC/A (60/40, dry weight % relation) constructs	932:993	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
28386058	1	60	from	problems	187:194	arg1	patients					208:215	numerous patients	199:215	numerous patients	199:215	Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients.
28386058	6	61	theme	cell	1027:1030	arg1	number					1032:1037	cell number	1027:1037	cell number within the cartilaginous tissue	1027:1069	Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing.
28386058	0	62	theme	Cells	58:62	arg1	Bioprinting					39:49	the 3D Bioprinting	32:49	the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink	32:97	Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink.
28386058	2	63	theme	lesions	253:259	arg1	treatment					235:243	a prospective treatment	221:243	a prospective treatment of such lesions	221:259	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	2	63	theme	lesions	253:259	arg1	cells					301:305	human-derived induced pluripotent stem cells	262:305	human-derived induced pluripotent stem cells (iPSCs)	262:313	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	1	64	theme	numerous	199:206	arg1	patients					208:215	numerous patients	199:215	numerous patients	199:215	Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients.
28386058	1	65	theme	secondary	136:144	arg1	osteoarthritis					146:159	secondary osteoarthritis	136:159	secondary osteoarthritis	136:159	Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients.
28386058	1	66	theme	clinical	178:185	arg1	problems					187:194	severe clinical problems	171:194	severe clinical problems in numerous patients	171:215	Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients.
28386058	0	67	theme	iPS	54:56	arg1	Cells					58:62	iPS Cells	54:62	iPS Cells	54:62	Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink.
28386058	2	68	theme	such	248:251	arg1	lesions					253:259	such lesions	248:259	such lesions	248:259	As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes.
28386058	4	69	theme	NFC/HA	652:657	arg1	case					644:647	the case	640:647	the case of NFC/HA	640:657	Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA.
28386058	6	70	from	importance	1136:1145	arg1	pursuit					1177:1183	the pursuit	1173:1183	the pursuit of achieving good survival after printing	1173:1225	Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing.
28386058	5	71	theme	3D-bioprinted	688:700	arg1	NFC/A					702:706	the 3D-bioprinted NFC/A	684:706	the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs	684:745	However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs.
24858090	3	0	theme	disproportionation	749:766	arg1	activities					768:777	disproportionation activities	749:777	disproportionation activities	749:777	The cyclization activities of these enzymes decreased, while disproportionation activities increased.
24858090	7	1	theme	Fusion	1337:1342	arg1	enzymes					1344:1350	Fusion enzymes	1337:1350	Fusion enzymes	1337:1350	Fusion enzymes, especially SAP6-CGTase, are promising starting points for further development through protein engineering.
24858090	7	1	theme	Fusion	1337:1342	arg1	SAP6-CGTase					1364:1374	SAP6-CGTase	1364:1374	especially SAP6-CGTase	1353:1374	Fusion enzymes, especially SAP6-CGTase, are promising starting points for further development through protein engineering.
24858090	7	1	theme	Fusion	1337:1342	arg1	points					1400:1405	promising starting points	1381:1405	promising starting points for further development through protein engineering	1381:1457	Fusion enzymes, especially SAP6-CGTase, are promising starting points for further development through protein engineering.
24858090	0	2	theme	acid	131:134	arg1	donor					182:186	the glycosyl donor	169:186	the glycosyl donor	169:186	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	0	2	theme	acid	131:134	arg1	synthesis					136:144	2-O-D-glucopyranosyl-L-ascorbic acid synthesis	99:144	2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch	99:164	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	2	3	theme	soluble	648:654	arg1	substrate					677:685	a substrate	675:685	a substrate	675:685	The results showed that two fusion enzymes, SAP5-CGTase and SAP6-CGTase, increased AA-2G yields to 2.33- and 3.36-fold that of wild-type CGTase when soluble starch was used as a substrate.
24858090	2	3	theme	soluble	648:654	arg1	starch					656:661	soluble starch	648:661	soluble starch	648:661	The results showed that two fusion enzymes, SAP5-CGTase and SAP6-CGTase, increased AA-2G yields to 2.33- and 3.36-fold that of wild-type CGTase when soluble starch was used as a substrate.
24858090	0	4	with	synthesis	136:144	arg1	starch					159:164	soluble starch	151:164	soluble starch	151:164	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	0	5	theme	soluble	151:157	arg1	starch					159:164	soluble starch	151:164	soluble starch	151:164	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	1	6	theme	cheap	409:413	arg1	starch					472:477	soluble starch	464:477	soluble starch	464:477	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	1	6	theme	cheap	409:413	arg1	maltodextrin					446:457	maltodextrin	446:457	maltodextrin	446:457	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	1	6	theme	cheap	409:413	arg1	substrates					415:424	cheap substrates	409:424	cheap substrates	409:424	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	1	6	theme	cheap	409:413	arg1	maltose					437:443	maltose	437:443	maltose	437:443	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	6	7	theme	AA-2G	1320:1324	arg1	synthesis					1326:1334	AA-2G synthesis	1320:1334	AA-2G synthesis	1320:1334	This study demonstrates an effective protein fusion approach to improving the substrate specificity of CGTase for AA-2G synthesis.
24858090	4	8	theme	enzymes	835:841	arg1	characterization					800:815	Enzymatic characterization	790:815	Enzymatic characterization of the two fusion enzymes	790:841	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	5	9	theme	bonds	1178:1182	arg1	number					1159:1164	the increased number	1145:1164	the increased number of hydrogen bonds present after fusion	1145:1203	As revealed by structure modeling of the fusion and wild-type CGTases, enhanced substrate-binding capacity may result from the increased number of hydrogen bonds present after fusion.
24858090	4	10	theme	SAP5-CGTase	952:962	arg1	specificity					937:947	the enhanced soluble starch specificity	909:947	the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase	909:978	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	4	11	theme	fusion	828:833	arg1	enzymes					835:841	the two fusion enzymes	820:841	the two fusion enzymes	820:841	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	5	12	theme	present	1184:1190	arg1	bonds					1178:1182	hydrogen bonds	1169:1182	hydrogen bonds present after fusion	1169:1203	As revealed by structure modeling of the fusion and wild-type CGTases, enhanced substrate-binding capacity may result from the increased number of hydrogen bonds present after fusion.
24858090	4	13	theme	enhanced	913:920	arg1	specificity					937:947	the enhanced soluble starch specificity	909:947	the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase	909:978	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	7	14	theme	protein	1439:1445	arg1	engineering					1447:1457	protein engineering	1439:1457	protein engineering	1439:1457	Fusion enzymes, especially SAP6-CGTase, are promising starting points for further development through protein engineering.
24858090	4	15	theme	starch	930:935	arg1	specificity					937:947	the enhanced soluble starch specificity	909:947	the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase	909:978	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	6	16	theme	fusion	1251:1256	arg1	approach					1258:1265	an effective protein fusion approach	1230:1265	an effective protein fusion approach to improving the substrate specificity of CGTase for AA-2G synthesis	1230:1334	This study demonstrates an effective protein fusion approach to improving the substrate specificity of CGTase for AA-2G synthesis.
24858090	1	17	theme	Paenibacillus	313:325	arg1	macerans					327:334	Paenibacillus macerans	313:334	Paenibacillus macerans	313:334	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	1	18	from	macerans	327:334	arg1	CGTase					300:305	cyclodextrin glycosyltransferase (CGTase)	266:306	cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans	266:334	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	6	19	theme	protein	1243:1249	arg1	approach					1258:1265	an effective protein fusion approach	1230:1265	an effective protein fusion approach to improving the substrate specificity of CGTase for AA-2G synthesis	1230:1334	This study demonstrates an effective protein fusion approach to improving the substrate specificity of CGTase for AA-2G synthesis.
24858090	1	20	theme	self-assembling	217:231	arg1	peptides					245:252	six self-assembling amphipathic peptides	213:252	six self-assembling amphipathic peptides (SAPs)	213:259	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	1	20	theme	self-assembling	217:231	arg1	SAPs					255:258	SAPs	255:258	SAPs	255:258	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	0	21	theme	amphipathic	26:36	arg1	oligopeptides					38:50	self-assembling amphipathic oligopeptides	10:50	self-assembling amphipathic oligopeptides	10:50	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	2	22	used	used	667:670	arg2	substrate					677:685	a substrate	675:685	a substrate	675:685	The results showed that two fusion enzymes, SAP5-CGTase and SAP6-CGTase, increased AA-2G yields to 2.33- and 3.36-fold that of wild-type CGTase when soluble starch was used as a substrate.
24858090	2	22	used	used	667:670	arg2	starch					656:661	soluble starch	648:661	soluble starch	648:661	The results showed that two fusion enzymes, SAP5-CGTase and SAP6-CGTase, increased AA-2G yields to 2.33- and 3.36-fold that of wild-type CGTase when soluble starch was used as a substrate.
24858090	0	23	theme	glycosyl	173:180	arg1	donor					182:186	the glycosyl donor	169:186	the glycosyl donor	169:186	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	0	23	theme	glycosyl	173:180	arg1	synthesis					136:144	2-O-D-glucopyranosyl-L-ascorbic acid synthesis	99:144	2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch	99:164	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	6	24	theme	effective	1233:1241	arg1	approach					1258:1265	an effective protein fusion approach	1230:1265	an effective protein fusion approach to improving the substrate specificity of CGTase for AA-2G synthesis	1230:1334	This study demonstrates an effective protein fusion approach to improving the substrate specificity of CGTase for AA-2G synthesis.
24858090	1	25	theme	amphipathic	233:243	arg1	peptides					245:252	six self-assembling amphipathic peptides	213:252	six self-assembling amphipathic peptides (SAPs)	213:259	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	1	25	theme	amphipathic	233:243	arg1	SAPs					255:258	SAPs	255:258	SAPs	255:258	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	0	26	theme	self-assembling	10:24	arg1	oligopeptides					38:50	self-assembling amphipathic oligopeptides	10:50	self-assembling amphipathic oligopeptides	10:50	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	5	27	theme	increased	1149:1157	arg1	number					1159:1164	the increased number	1145:1164	the increased number of hydrogen bonds present after fusion	1145:1203	As revealed by structure modeling of the fusion and wild-type CGTases, enhanced substrate-binding capacity may result from the increased number of hydrogen bonds present after fusion.
24858090	7	28	theme	further	1411:1417	arg1	development					1419:1429	further development	1411:1429	further development through protein engineering	1411:1457	Fusion enzymes, especially SAP6-CGTase, are promising starting points for further development through protein engineering.
24858090	2	29	theme	fusion	527:532	arg1	SAP5-CGTase					543:553	SAP5-CGTase	543:553	SAP5-CGTase	543:553	The results showed that two fusion enzymes, SAP5-CGTase and SAP6-CGTase, increased AA-2G yields to 2.33- and 3.36-fold that of wild-type CGTase when soluble starch was used as a substrate.
24858090	2	29	theme	fusion	527:532	arg1	enzymes					534:540	two fusion enzymes	523:540	two fusion enzymes	523:540	The results showed that two fusion enzymes, SAP5-CGTase and SAP6-CGTase, increased AA-2G yields to 2.33- and 3.36-fold that of wild-type CGTase when soluble starch was used as a substrate.
24858090	2	29	theme	fusion	527:532	arg1	SAP6-CGTase					559:569	SAP6-CGTase	559:569	SAP6-CGTase	559:569	The results showed that two fusion enzymes, SAP5-CGTase and SAP6-CGTase, increased AA-2G yields to 2.33- and 3.36-fold that of wild-type CGTase when soluble starch was used as a substrate.
24858090	5	30	theme	CGTases	1084:1090	arg1	modeling					1047:1054	structure modeling	1037:1054	structure modeling of the fusion and wild-type CGTases	1037:1090	As revealed by structure modeling of the fusion and wild-type CGTases, enhanced substrate-binding capacity may result from the increased number of hydrogen bonds present after fusion.
24858090	3	31	theme	cyclization	692:702	arg1	activities					704:713	The cyclization activities	688:713	The cyclization activities of these enzymes	688:730	The cyclization activities of these enzymes decreased, while disproportionation activities increased.
24858090	4	32	theme	AA-2G	883:887	arg1	synthesis					889:897	AA-2G synthesis	883:897	AA-2G synthesis	883:897	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	6	33	theme	substrate	1284:1292	arg1	specificity					1294:1304	the substrate specificity	1280:1304	the substrate specificity of CGTase for AA-2G synthesis	1280:1334	This study demonstrates an effective protein fusion approach to improving the substrate specificity of CGTase for AA-2G synthesis.
24858090	7	34	theme	starting	1391:1398	arg1	points					1400:1405	promising starting points	1381:1405	promising starting points for further development through protein engineering	1381:1457	Fusion enzymes, especially SAP6-CGTase, are promising starting points for further development through protein engineering.
24858090	7	34	theme	starting	1391:1398	arg1	enzymes					1344:1350	Fusion enzymes	1337:1350	Fusion enzymes	1337:1350	Fusion enzymes, especially SAP6-CGTase, are promising starting points for further development through protein engineering.
24858090	0	35	theme	oligopeptides	38:50	arg1	Fusion					0:5	Fusion	0:5	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase	0:88	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	1	36	theme	2-O-D-glucopyranosyl-L-ascorbic	348:378	arg1	production					393:402	2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production	348:402	2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production	348:402	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	4	37	theme	wild-type	1004:1012	arg1	CGTase					1014:1019	the wild-type CGTase	1000:1019	the wild-type CGTase	1000:1019	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	5	38	theme	enhanced	1093:1100	arg1	capacity					1120:1127	enhanced substrate-binding capacity	1093:1127	enhanced substrate-binding capacity	1093:1127	As revealed by structure modeling of the fusion and wild-type CGTases, enhanced substrate-binding capacity may result from the increased number of hydrogen bonds present after fusion.
24858090	1	39	theme	acid	380:383	arg1	production					393:402	2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production	348:402	2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production	348:402	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	2	40	theme	wild-type	626:634	arg1	CGTase					636:641	wild-type CGTase	626:641	wild-type CGTase	626:641	The results showed that two fusion enzymes, SAP5-CGTase and SAP6-CGTase, increased AA-2G yields to 2.33- and 3.36-fold that of wild-type CGTase when soluble starch was used as a substrate.
24858090	5	41	theme	hydrogen	1169:1176	arg1	bonds					1178:1182	hydrogen bonds	1169:1182	hydrogen bonds present after fusion	1169:1203	As revealed by structure modeling of the fusion and wild-type CGTases, enhanced substrate-binding capacity may result from the increased number of hydrogen bonds present after fusion.
24858090	5	42	theme	substrate-binding	1102:1118	arg1	capacity					1120:1127	enhanced substrate-binding capacity	1093:1127	enhanced substrate-binding capacity	1093:1127	As revealed by structure modeling of the fusion and wild-type CGTases, enhanced substrate-binding capacity may result from the increased number of hydrogen bonds present after fusion.
24858090	4	43	theme	soluble	922:928	arg1	specificity					937:947	the enhanced soluble starch specificity	909:947	the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase	909:978	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	0	44	theme	cyclodextrin	57:68	arg1	glycosyltransferase					70:88	cyclodextrin glycosyltransferase	57:88	cyclodextrin glycosyltransferase	57:88	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	4	45	theme	SAP6-CGTase	968:978	arg1	specificity					937:947	the enhanced soluble starch specificity	909:947	the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase	909:978	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	1	46	theme	soluble	464:470	arg1	starch					472:477	soluble starch	464:477	soluble starch	464:477	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	4	47	theme	synthesis	889:897	arg1	analysis					871:878	kinetics analysis	862:878	kinetics analysis of AA-2G synthesis	862:897	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	7	48	theme	promising	1381:1389	arg1	points					1400:1405	promising starting points	1381:1405	promising starting points for further development through protein engineering	1381:1457	Fusion enzymes, especially SAP6-CGTase, are promising starting points for further development through protein engineering.
24858090	7	48	theme	promising	1381:1389	arg1	enzymes					1344:1350	Fusion enzymes	1337:1350	Fusion enzymes	1337:1350	Fusion enzymes, especially SAP6-CGTase, are promising starting points for further development through protein engineering.
24858090	1	49	theme	AA-2G	386:390	arg1	production					393:402	2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production	348:402	2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production	348:402	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	3	50	theme	enzymes	724:730	arg1	activities					704:713	The cyclization activities	688:713	The cyclization activities of these enzymes	688:730	The cyclization activities of these enzymes decreased, while disproportionation activities increased.
24858090	5	51	theme	fusion	1063:1068	arg1	CGTases					1084:1090	the fusion and wild-type CGTases	1059:1090	CGTases	1084:1090	As revealed by structure modeling of the fusion and wild-type CGTases, enhanced substrate-binding capacity may result from the increased number of hydrogen bonds present after fusion.
24858090	0	52	with	Fusion	0:5	arg1	glycosyltransferase					70:88	cyclodextrin glycosyltransferase	57:88	cyclodextrin glycosyltransferase	57:88	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	4	53	theme	Enzymatic	790:798	arg1	characterization					800:815	Enzymatic characterization	790:815	Enzymatic characterization of the two fusion enzymes	790:841	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	5	54	theme	structure	1037:1045	arg1	modeling					1047:1054	structure modeling	1037:1054	structure modeling of the fusion and wild-type CGTases	1037:1090	As revealed by structure modeling of the fusion and wild-type CGTases, enhanced substrate-binding capacity may result from the increased number of hydrogen bonds present after fusion.
24858090	0	55	theme	2-O-D-glucopyranosyl-L-ascorbic	99:129	arg1	donor					182:186	the glycosyl donor	169:186	the glycosyl donor	169:186	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	0	55	theme	2-O-D-glucopyranosyl-L-ascorbic	99:129	arg1	synthesis					136:144	2-O-D-glucopyranosyl-L-ascorbic acid synthesis	99:144	2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch	99:164	Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.
24858090	1	56	theme	glycosyl	482:489	arg1	donors					491:496	glycosyl donors	482:496	glycosyl donors	482:496	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	5	57	theme	wild-type	1074:1082	arg1	CGTases					1084:1090	the fusion and wild-type CGTases	1059:1090	CGTases	1084:1090	As revealed by structure modeling of the fusion and wild-type CGTases, enhanced substrate-binding capacity may result from the increased number of hydrogen bonds present after fusion.
24858090	2	58	theme	AA-2G	582:586	arg1	yields					588:593	AA-2G yields	582:593	AA-2G yields to 2.33-	582:602	The results showed that two fusion enzymes, SAP5-CGTase and SAP6-CGTase, increased AA-2G yields to 2.33- and 3.36-fold that of wild-type CGTase when soluble starch was used as a substrate.
24858090	1	59	theme	cyclodextrin	266:277	arg1	CGTase					300:305	cyclodextrin glycosyltransferase (CGTase)	266:306	cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans	266:334	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
24858090	4	60	theme	kinetics	862:869	arg1	analysis					871:878	kinetics analysis	862:878	kinetics analysis of AA-2G synthesis	862:897	Enzymatic characterization of the two fusion enzymes was performed, and kinetics analysis of AA-2G synthesis confirmed the enhanced soluble starch specificity of SAP5-CGTase and SAP6-CGTase compared to that in the wild-type CGTase.
24858090	6	61	theme	CGTase	1309:1314	arg1	specificity					1294:1304	the substrate specificity	1280:1304	the substrate specificity of CGTase for AA-2G synthesis	1280:1334	This study demonstrates an effective protein fusion approach to improving the substrate specificity of CGTase for AA-2G synthesis.
24858090	1	62	theme	glycosyltransferase	279:297	arg1	CGTase					300:305	cyclodextrin glycosyltransferase (CGTase)	266:306	cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans	266:334	In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors.
26256351	0	0	theme	single	79:84	arg1	line					91:94	a single cell line	77:94	a single cell line	77:94	Innovative production of bio-cellulose using a cell-free system derived from a single cell line.
26256351	7	1	theme	cellulose-producing	1078:1096	arg1	cells					1098:1102	cellulose-producing cells	1078:1102	cellulose-producing cells	1078:1102	This approach can overcome some limitations of cellulose-producing cells and offers a wider scope for synthesizing cellulose composites with bactericidal elements through in situ synthesizing approaches.
26256351	3	2	theme	chromatography	442:455	arg1	spectrometry					480:491	liquid chromatography mass spectrometry/mass spectrometry	435:491	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	7	3	theme	in	1202:1203	arg1	approaches					1223:1232	in situ synthesizing approaches	1202:1232	in situ synthesizing approaches	1202:1232	This approach can overcome some limitations of cellulose-producing cells and offers a wider scope for synthesizing cellulose composites with bactericidal elements through in situ synthesizing approaches.
26256351	7	4	theme	cells	1098:1102	arg1	limitations					1063:1073	some limitations	1058:1073	some limitations of cellulose-producing cells	1058:1102	This approach can overcome some limitations of cellulose-producing cells and offers a wider scope for synthesizing cellulose composites with bactericidal elements through in situ synthesizing approaches.
26256351	3	5	theme	mass	457:460	arg1	spectrometry					480:491	liquid chromatography mass spectrometry/mass spectrometry	435:491	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	7	6	theme	bactericidal	1172:1183	arg1	elements					1185:1192	bactericidal elements	1172:1192	bactericidal elements through in situ synthesizing approaches	1172:1232	This approach can overcome some limitations of cellulose-producing cells and offers a wider scope for synthesizing cellulose composites with bactericidal elements through in situ synthesizing approaches.
26256351	2	7	theme	cells	315:319	arg1	disruption					301:310	the complete disruption	288:310	the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min	288:352	Microscopic analysis indicated the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min that added 95.12 μg/mL protein, 1.63 mM ATP, and 1.11 mM NADH into the medium.
26256351	0	8	theme	cell	86:89	arg1	line					91:94	a single cell line	77:94	a single cell line	77:94	Innovative production of bio-cellulose using a cell-free system derived from a single cell line.
26256351	5	9	theme	consumed	836:843	arg1	glucose					845:851	consumed glucose	836:851	consumed glucose	836:851	The improved yield based on consumed glucose indicated the superiority of cell-free system.
26256351	4	10	dep	yield	773:777	arg1	%					771:771	%	771:771	%	771:771	Under static conditions at 30 °C, microbial and cell-free systems produced 3.78 and 3.72 g/L cellulose, corresponding to 39.62 and 57.68% yield, respectively after 15 days.
26256351	3	11	theme	linear	493:498	arg1	analysis					540:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis	433:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	5	12	theme	improved	812:819	arg1	yield					821:825	The improved yield	808:825	The improved yield based on consumed glucose	808:851	The improved yield based on consumed glucose indicated the superiority of cell-free system.
26256351	2	13	theme	μg/mL	371:375	arg1	protein					377:383	95.12 μg/mL protein	365:383	95.12 μg/mL protein	365:383	Microscopic analysis indicated the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min that added 95.12 μg/mL protein, 1.63 mM ATP, and 1.11 mM NADH into the medium.
26256351	0	14	theme	cell-free	47:55	arg1	system					57:62	a cell-free system	45:62	a cell-free system derived from a single cell line	45:94	Innovative production of bio-cellulose using a cell-free system derived from a single cell line.
26256351	7	15	theme	cellulose	1146:1154	arg1	composites					1156:1165	cellulose composites	1146:1165	cellulose composites	1146:1165	This approach can overcome some limitations of cellulose-producing cells and offers a wider scope for synthesizing cellulose composites with bactericidal elements through in situ synthesizing approaches.
26256351	6	16	from	synthesis	996:1004	arg1	system					1023:1028	the cell-free system	1009:1028	the cell-free system	1009:1028	Based on current findings and literature, we hypothesized a synthetic pathway for bio-cellulose synthesis in the cell-free system.
26256351	2	17	theme	complete	292:299	arg1	disruption					301:310	the complete disruption	288:310	the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min	288:352	Microscopic analysis indicated the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min that added 95.12 μg/mL protein, 1.63 mM ATP, and 1.11 mM NADH into the medium.
26256351	1	18	theme	cell-free	163:171	arg1	system					173:178	a cell-free system	161:178	a cell-free system developed by disrupting Gluconacetobacter hansenii PJK through bead-beating	161:254	The current study was intended to produce bio-cellulose through a cell-free system developed by disrupting Gluconacetobacter hansenii PJK through bead-beating.
26256351	2	19	theme	1.11	403:406	arg1	mM					408:409	mM	408:409	mM	408:409	Microscopic analysis indicated the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min that added 95.12 μg/mL protein, 1.63 mM ATP, and 1.11 mM NADH into the medium.
26256351	5	20	theme	cell-free	882:890	arg1	system					892:897	cell-free system	882:897	cell-free system	882:897	The improved yield based on consumed glucose indicated the superiority of cell-free system.
26256351	4	21	theme	static	641:646	arg1	conditions					648:657	static conditions	641:657	static conditions at 30 °C	641:666	Under static conditions at 30 °C, microbial and cell-free systems produced 3.78 and 3.72 g/L cellulose, corresponding to 39.62 and 57.68% yield, respectively after 15 days.
26256351	0	22	theme	Innovative	0:9	arg1	production					11:20	Innovative production	0:20	Innovative production of bio-cellulose	0:37	Innovative production of bio-cellulose using a cell-free system derived from a single cell line.
26256351	4	23	dep	produced	701:708	arg1	corresponding					739:751	corresponding	739:751	corresponding	739:751	Under static conditions at 30 °C, microbial and cell-free systems produced 3.78 and 3.72 g/L cellulose, corresponding to 39.62 and 57.68% yield, respectively after 15 days.
26256351	3	24	theme	Orbitrap	531:538	arg1	analysis					540:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis	433:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	4	25	theme	g/L	724:726	arg1	cellulose					728:736	3.78 and 3.72 g/L cellulose	710:736	3.78 and 3.72 g/L cellulose	710:736	Under static conditions at 30 °C, microbial and cell-free systems produced 3.78 and 3.72 g/L cellulose, corresponding to 39.62 and 57.68% yield, respectively after 15 days.
26256351	6	26	theme	cell-free	1013:1021	arg1	system					1023:1028	the cell-free system	1009:1028	the cell-free system	1009:1028	Based on current findings and literature, we hypothesized a synthetic pathway for bio-cellulose synthesis in the cell-free system.
26256351	0	27	theme	bio-cellulose	25:37	arg1	production					11:20	Innovative production	0:20	Innovative production of bio-cellulose	0:37	Innovative production of bio-cellulose using a cell-free system derived from a single cell line.
26256351	3	28	theme	LC-MS/MS	517:524	arg1	analysis					540:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis	433:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	2	29	theme	mM	391:392	arg1	ATP					394:396	1.63 mM ATP	386:396	1.63 mM ATP	386:396	Microscopic analysis indicated the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min that added 95.12 μg/mL protein, 1.63 mM ATP, and 1.11 mM NADH into the medium.
26256351	5	30	theme	system	892:897	arg1	superiority					867:877	the superiority	863:877	the superiority of cell-free system	863:897	The improved yield based on consumed glucose indicated the superiority of cell-free system.
26256351	6	31	theme	current	909:915	arg1	findings					917:924	current findings	909:924	current findings	909:924	Based on current findings and literature, we hypothesized a synthetic pathway for bio-cellulose synthesis in the cell-free system.
26256351	7	32	dep	in	1202:1203	arg1	situ					1205:1208	situ	1205:1208	situ	1205:1208	This approach can overcome some limitations of cellulose-producing cells and offers a wider scope for synthesizing cellulose composites with bactericidal elements through in situ synthesizing approaches.
26256351	3	33	theme	LTQ	526:528	arg1	analysis					540:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis	433:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	2	34	theme	1.63	386:389	arg1	mM					391:392	mM	391:392	mM	391:392	Microscopic analysis indicated the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min that added 95.12 μg/mL protein, 1.63 mM ATP, and 1.11 mM NADH into the medium.
26256351	3	35	theme	bio-cellulose	610:622	arg1	synthesis					624:632	bio-cellulose synthesis	610:632	bio-cellulose synthesis	610:632	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	3	36	theme	cell-lysate	552:562	arg1	analysis					540:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis	433:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	1	37	theme	Gluconacetobacter	204:220	arg1	PJK					231:233	Gluconacetobacter hansenii PJK	204:233	Gluconacetobacter hansenii PJK	204:233	The current study was intended to produce bio-cellulose through a cell-free system developed by disrupting Gluconacetobacter hansenii PJK through bead-beating.
26256351	7	38	theme	wider	1117:1121	arg1	scope					1123:1127	a wider scope	1115:1127	a wider scope for synthesizing cellulose composites with bactericidal elements through in situ synthesizing approaches	1115:1232	This approach can overcome some limitations of cellulose-producing cells and offers a wider scope for synthesizing cellulose composites with bactericidal elements through in situ synthesizing approaches.
26256351	3	39	theme	key	594:596	arg1	enzymes					598:604	all key enzymes	590:604	all key enzymes	590:604	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	6	40	theme	synthetic	960:968	arg1	pathway					970:976	a synthetic pathway	958:976	a synthetic pathway for bio-cellulose synthesis in the cell-free system	958:1028	Based on current findings and literature, we hypothesized a synthetic pathway for bio-cellulose synthesis in the cell-free system.
26256351	0	41	attach	derived	64:70	arg2	system					57:62	a cell-free system	45:62	a cell-free system derived from a single cell line	45:94	Innovative production of bio-cellulose using a cell-free system derived from a single cell line.
26256351	0	41	attach	derived	64:70	arg1	line					91:94	a single cell line	77:94	a single cell line	77:94	Innovative production of bio-cellulose using a cell-free system derived from a single cell line.
26256351	1	42	theme	hansenii	222:229	arg1	PJK					231:233	Gluconacetobacter hansenii PJK	204:233	Gluconacetobacter hansenii PJK	204:233	The current study was intended to produce bio-cellulose through a cell-free system developed by disrupting Gluconacetobacter hansenii PJK through bead-beating.
26256351	3	43	theme	enzymes	598:604	arg1	presence					578:585	the presence	574:585	the presence of all key enzymes for bio-cellulose synthesis	574:632	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	1	44	theme	current	101:107	arg1	study					109:113	The current study	97:113	The current study	97:113	The current study was intended to produce bio-cellulose through a cell-free system developed by disrupting Gluconacetobacter hansenii PJK through bead-beating.
26256351	2	45	theme	mM	408:409	arg1	NADH					411:414	1.11 mM NADH	403:414	1.11 mM NADH	403:414	Microscopic analysis indicated the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min that added 95.12 μg/mL protein, 1.63 mM ATP, and 1.11 mM NADH into the medium.
26256351	3	46	theme	liquid	435:440	arg1	spectrometry					480:491	liquid chromatography mass spectrometry/mass spectrometry	435:491	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	2	47	from	disruption	301:310	arg1	min					350:352	20 min	347:352	20 min	347:352	Microscopic analysis indicated the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min that added 95.12 μg/mL protein, 1.63 mM ATP, and 1.11 mM NADH into the medium.
26256351	2	48	dep	10	328:329	arg1	7					331:331	7	331:331	7	331:331	Microscopic analysis indicated the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min that added 95.12 μg/mL protein, 1.63 mM ATP, and 1.11 mM NADH into the medium.
26256351	3	49	theme	trap	500:503	arg1	analysis					540:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis	433:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	3	50	theme	quadrupole	505:514	arg1	analysis					540:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis	433:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	4	51	theme	cell-free	683:691	arg1	systems					693:699	cell-free systems	683:699	cell-free systems	683:699	Under static conditions at 30 °C, microbial and cell-free systems produced 3.78 and 3.72 g/L cellulose, corresponding to 39.62 and 57.68% yield, respectively after 15 days.
26256351	6	52	theme	bio-cellulose	982:994	arg1	synthesis					996:1004	bio-cellulose synthesis	982:1004	bio-cellulose synthesis in the cell-free system	982:1028	Based on current findings and literature, we hypothesized a synthetic pathway for bio-cellulose synthesis in the cell-free system.
26256351	3	53	theme	spectrometry/mass	462:478	arg1	spectrometry					480:491	liquid chromatography mass spectrometry/mass spectrometry	435:491	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	2	54	theme	95.12	365:369	arg1	protein					377:383	95.12 μg/mL protein	365:383	95.12 μg/mL protein	365:383	Microscopic analysis indicated the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min that added 95.12 μg/mL protein, 1.63 mM ATP, and 1.11 mM NADH into the medium.
26256351	4	55	dep	%	771:771	arg1	39.62					756:760	39.62	756:760	39.62	756:760	Under static conditions at 30 °C, microbial and cell-free systems produced 3.78 and 3.72 g/L cellulose, corresponding to 39.62 and 57.68% yield, respectively after 15 days.
26256351	4	55	dep	%	771:771	arg1	57.68					766:770	57.68	766:770	57.68	766:770	Under static conditions at 30 °C, microbial and cell-free systems produced 3.78 and 3.72 g/L cellulose, corresponding to 39.62 and 57.68% yield, respectively after 15 days.
26256351	7	56	theme	synthesizing	1210:1221	arg1	approaches					1223:1232	in situ synthesizing approaches	1202:1232	in situ synthesizing approaches	1202:1232	This approach can overcome some limitations of cellulose-producing cells and offers a wider scope for synthesizing cellulose composites with bactericidal elements through in situ synthesizing approaches.
26256351	2	57	theme	Microscopic	257:267	arg1	analysis					269:276	Microscopic analysis	257:276	Microscopic analysis	257:276	Microscopic analysis indicated the complete disruption of cells (2.6 × 10(7) cells/mL) in 20 min that added 95.12 μg/mL protein, 1.63 mM ATP, and 1.11 mM NADH into the medium.
26256351	3	58	theme	spectrometry	480:491	arg1	analysis					540:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis	433:547	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate	433:562	A liquid chromatography mass spectrometry/mass spectrometry linear trap quadrupole (LC-MS/MS LTQ) Orbitrap analysis of cell-lysate confirmed the presence of all key enzymes for bio-cellulose synthesis.
26256351	4	59	from	°C	665:666	arg1	conditions					648:657	static conditions	641:657	static conditions at 30 °C	641:666	Under static conditions at 30 °C, microbial and cell-free systems produced 3.78 and 3.72 g/L cellulose, corresponding to 39.62 and 57.68% yield, respectively after 15 days.
25053175	0	0	theme	inclusion	89:97	arg1	complexation					99:110	host-guest inclusion complexation	78:110	host-guest inclusion complexation	78:110	Fabrication of a photocontrolled surface with switchable wettability based on host-guest inclusion complexation and protein resistance.
25053175	7	1	contain	possesses	1303:1311	arg2	properties					1336:1345	good protein-resistant properties	1313:1345	good protein-resistant properties	1313:1345	The results demonstrate that the surface modified with PEG possesses good protein-resistant properties.
25053175	7	1	contain	possesses	1303:1311	arg1	surface					1277:1283	the surface	1273:1283	the surface modified with PEG	1273:1301	The results demonstrate that the surface modified with PEG possesses good protein-resistant properties.
25053175	6	2	theme	adsorption	1162:1171	arg1	assay					1173:1177	a protein adsorption assay	1152:1177	a protein adsorption assay using bovine serum albumin, which was also characterized by XPS	1152:1241	The antifouling property of the PEG-functionalized surface was evaluated by a protein adsorption assay using bovine serum albumin, which was also characterized by XPS.
25053175	0	3	theme	host-guest	78:87	arg1	complexation					99:110	host-guest inclusion complexation	78:110	host-guest inclusion complexation	78:110	Fabrication of a photocontrolled surface with switchable wettability based on host-guest inclusion complexation and protein resistance.
25053175	5	4	theme	X-ray	965:969	arg1	spectroscopy					985:996	X-ray photoelectron spectroscopy	965:996	X-ray photoelectron spectroscopy (XPS)	965:1002	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	5	4	theme	X-ray	965:969	arg1	XPS					999:1001	XPS	999:1001	XPS	999:1001	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	3	5	attach	attached	496:503	arg2	series					446:451	a series	444:451	a series of polymers with different polarities	444:489	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	3	5	attach	attached	496:503	arg1	β-cyclodextrin					522:535	host molecule β-cyclodextrin	508:535	host molecule β-cyclodextrin (β-CD)	508:542	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	3	5	attach	attached	496:503	arg1	β-CD					538:541	β-CD	538:541	β-CD	538:541	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	2	6	theme	silicon	333:339	arg1	wafer					341:345	The silicon wafer	329:345	The silicon wafer	329:345	The silicon wafer was first modified by guest molecule azobenzene (Azo) via a silanization reaction.
25053175	1	7	theme	host-guest	294:303	arg1	complexation					315:326	host-guest inclusion complexation	294:326	host-guest inclusion complexation	294:326	A novel surface-modification strategy has been developed for the construction of a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation.
25053175	7	8	theme	good	1313:1316	arg1	properties					1336:1345	good protein-resistant properties	1313:1345	good protein-resistant properties	1313:1345	The results demonstrate that the surface modified with PEG possesses good protein-resistant properties.
25053175	1	9	theme	inclusion	305:313	arg1	complexation					315:326	host-guest inclusion complexation	294:326	host-guest inclusion complexation	294:326	A novel surface-modification strategy has been developed for the construction of a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation.
25053175	5	10	theme	photoelectron	971:983	arg1	spectroscopy					985:996	X-ray photoelectron spectroscopy	965:996	X-ray photoelectron spectroscopy (XPS)	965:1002	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	5	10	theme	photoelectron	971:983	arg1	XPS					999:1001	XPS	999:1001	XPS	999:1001	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	4	11	theme	photocontrolled	627:641	arg1	surface					657:663	a photocontrolled silicon wafer surface	625:663	a photocontrolled silicon wafer surface modified with polymers	625:686	Finally, a photocontrolled silicon wafer surface modified with polymers was fabricated by inclusion complexation between β-CD and Azo, and the surface properties of the substrate are dependent on the polymers we used.
25053175	3	12	theme	β-CD-containing	555:569	arg1	polymers					586:593	β-CD-containing hemitelechelic polymers	555:593	β-CD-containing hemitelechelic polymers	555:593	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	5	13	theme	modified	925:932	arg1	surfaces					934:941	the modified surfaces	921:941	the modified surfaces	921:941	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	6	14	theme	PEG-functionalized	1108:1125	arg1	surface					1127:1133	the PEG-functionalized surface	1104:1133	the PEG-functionalized surface	1104:1133	The antifouling property of the PEG-functionalized surface was evaluated by a protein adsorption assay using bovine serum albumin, which was also characterized by XPS.
25053175	0	15	theme	protein	116:122	arg1	resistance					124:133	protein resistance	116:133	protein resistance	116:133	Fabrication of a photocontrolled surface with switchable wettability based on host-guest inclusion complexation and protein resistance.
25053175	6	16	theme	serum	1192:1196	arg1	albumin					1198:1204	bovine serum albumin	1185:1204	bovine serum albumin	1185:1204	The antifouling property of the PEG-functionalized surface was evaluated by a protein adsorption assay using bovine serum albumin, which was also characterized by XPS.
25053175	3	17	theme	hemitelechelic	571:584	arg1	polymers					586:593	β-CD-containing hemitelechelic polymers	555:593	β-CD-containing hemitelechelic polymers	555:593	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	6	18	theme	protein	1154:1160	arg1	assay					1173:1177	a protein adsorption assay	1152:1177	a protein adsorption assay using bovine serum albumin, which was also characterized by XPS	1152:1241	The antifouling property of the PEG-functionalized surface was evaluated by a protein adsorption assay using bovine serum albumin, which was also characterized by XPS.
25053175	3	19	theme	different	470:478	arg1	polarities					480:489	different polarities	470:489	different polarities	470:489	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	4	20	theme	inclusion	706:714	arg1	complexation					716:727	inclusion complexation	706:727	inclusion complexation between β-CD and Azo	706:748	Finally, a photocontrolled silicon wafer surface modified with polymers was fabricated by inclusion complexation between β-CD and Azo, and the surface properties of the substrate are dependent on the polymers we used.
25053175	4	21	theme	substrate	785:793	arg1	dependent					799:807	dependent	799:807	dependent	799:807	Finally, a photocontrolled silicon wafer surface modified with polymers was fabricated by inclusion complexation between β-CD and Azo, and the surface properties of the substrate are dependent on the polymers we used.
25053175	4	21	theme	substrate	785:793	arg1	properties					767:776	the surface properties	755:776	the surface properties of the substrate	755:793	Finally, a photocontrolled silicon wafer surface modified with polymers was fabricated by inclusion complexation between β-CD and Azo, and the surface properties of the substrate are dependent on the polymers we used.
25053175	3	22	with	polymers	456:463	arg1	polarities					480:489	different polarities	470:489	different polarities	470:489	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	2	23	theme	silanization	407:418	arg1	reaction					420:427	a silanization reaction	405:427	a silanization reaction	405:427	The silicon wafer was first modified by guest molecule azobenzene (Azo) via a silanization reaction.
25053175	6	24	theme	antifouling	1080:1090	arg1	property					1092:1099	The antifouling property	1076:1099	The antifouling property of the PEG-functionalized surface	1076:1133	The antifouling property of the PEG-functionalized surface was evaluated by a protein adsorption assay using bovine serum albumin, which was also characterized by XPS.
25053175	3	25	theme	polymers	456:463	arg1	series					446:451	a series	444:451	a series of polymers with different polarities	444:489	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	0	26	theme	photocontrolled	17:31	arg1	surface					33:39	a photocontrolled surface	15:39	a photocontrolled surface	15:39	Fabrication of a photocontrolled surface with switchable wettability based on host-guest inclusion complexation and protein resistance.
25053175	2	27	mod	modified	357:364	arg3	azobenzene					384:393	guest molecule azobenzene	369:393	guest molecule azobenzene (Azo)	369:399	The silicon wafer was first modified by guest molecule azobenzene (Azo) via a silanization reaction.
25053175	2	27	mod	modified	357:364	arg1	wafer					341:345	The silicon wafer	329:345	The silicon wafer	329:345	The silicon wafer was first modified by guest molecule azobenzene (Azo) via a silanization reaction.
25053175	2	27	mod	modified	357:364	arg3	Azo					396:398	Azo	396:398	Azo	396:398	The silicon wafer was first modified by guest molecule azobenzene (Azo) via a silanization reaction.
25053175	3	28	theme	click	599:603	arg1	chemistry					605:613	click chemistry	599:613	click chemistry	599:613	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	6	29	theme	surface	1127:1133	arg1	property					1092:1099	The antifouling property	1076:1099	The antifouling property of the PEG-functionalized surface	1076:1133	The antifouling property of the PEG-functionalized surface was evaluated by a protein adsorption assay using bovine serum albumin, which was also characterized by XPS.
25053175	6	30	theme	bovine	1185:1190	arg1	albumin					1198:1204	bovine serum albumin	1185:1204	bovine serum albumin	1185:1204	The antifouling property of the PEG-functionalized surface was evaluated by a protein adsorption assay using bovine serum albumin, which was also characterized by XPS.
25053175	4	31	theme	wafer	651:655	arg1	surface					657:663	a photocontrolled silicon wafer surface	625:663	a photocontrolled silicon wafer surface modified with polymers	625:686	Finally, a photocontrolled silicon wafer surface modified with polymers was fabricated by inclusion complexation between β-CD and Azo, and the surface properties of the substrate are dependent on the polymers we used.
25053175	5	32	theme	force	1012:1016	arg1	microscope					1018:1027	atomic force microscope	1005:1027	atomic force microscope	1005:1027	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	1	33	theme	photocontrolled	219:233	arg1	surface					249:255	a photocontrolled silicon wafer surface	217:255	a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation	217:326	A novel surface-modification strategy has been developed for the construction of a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation.
25053175	5	34	theme	hydrophilic/hydrophobic	885:907	arg1	property					909:916	hydrophilic/hydrophobic property	885:916	hydrophilic/hydrophobic property	885:916	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	5	35	theme	surfaces	934:941	arg1	property					909:916	hydrophilic/hydrophobic property	885:916	hydrophilic/hydrophobic property	885:916	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	5	35	theme	surfaces	934:941	arg1	composition					848:858	The elemental composition	834:858	The elemental composition	834:858	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	5	35	theme	surfaces	934:941	arg1	morphology					869:878	surface morphology	861:878	surface morphology	861:878	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	1	36	theme	silicon	235:241	arg1	surface					249:255	a photocontrolled silicon wafer surface	217:255	a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation	217:326	A novel surface-modification strategy has been developed for the construction of a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation.
25053175	0	37	theme	surface	33:39	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of a photocontrolled surface with switchable wettability	0:67	Fabrication of a photocontrolled surface with switchable wettability based on host-guest inclusion complexation and protein resistance.
25053175	5	38	theme	surface	861:867	arg1	morphology					869:878	surface morphology	861:878	surface morphology	861:878	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	1	39	theme	wafer	243:247	arg1	surface					249:255	a photocontrolled silicon wafer surface	217:255	a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation	217:326	A novel surface-modification strategy has been developed for the construction of a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation.
25053175	5	40	theme	elemental	838:846	arg1	composition					848:858	The elemental composition	834:858	The elemental composition	834:858	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	4	41	theme	surface	759:765	arg1	dependent					799:807	dependent	799:807	dependent	799:807	Finally, a photocontrolled silicon wafer surface modified with polymers was fabricated by inclusion complexation between β-CD and Azo, and the surface properties of the substrate are dependent on the polymers we used.
25053175	4	41	theme	surface	759:765	arg1	properties					767:776	the surface properties	755:776	the surface properties of the substrate	755:793	Finally, a photocontrolled silicon wafer surface modified with polymers was fabricated by inclusion complexation between β-CD and Azo, and the surface properties of the substrate are dependent on the polymers we used.
25053175	0	42	theme	switchable	46:55	arg1	wettability					57:67	switchable wettability	46:67	switchable wettability	46:67	Fabrication of a photocontrolled surface with switchable wettability based on host-guest inclusion complexation and protein resistance.
25053175	3	43	theme	host	508:511	arg1	β-cyclodextrin					522:535	host molecule β-cyclodextrin	508:535	host molecule β-cyclodextrin (β-CD)	508:542	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	3	43	theme	host	508:511	arg1	β-CD					538:541	β-CD	538:541	β-CD	538:541	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	2	44	theme	molecule	375:382	arg1	Azo					396:398	Azo	396:398	Azo	396:398	The silicon wafer was first modified by guest molecule azobenzene (Azo) via a silanization reaction.
25053175	2	44	theme	molecule	375:382	arg1	azobenzene					384:393	guest molecule azobenzene	369:393	guest molecule azobenzene (Azo)	369:399	The silicon wafer was first modified by guest molecule azobenzene (Azo) via a silanization reaction.
25053175	5	45	theme	angle	1042:1046	arg1	measurements					1048:1059	contact angle measurements	1034:1059	contact angle measurements	1034:1059	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	0	46	with	Fabrication	0:10	arg1	wettability					57:67	switchable wettability	46:67	switchable wettability	46:67	Fabrication of a photocontrolled surface with switchable wettability based on host-guest inclusion complexation and protein resistance.
25053175	1	47	theme	novel	138:142	arg1	strategy					165:172	A novel surface-modification strategy	136:172	A novel surface-modification strategy	136:172	A novel surface-modification strategy has been developed for the construction of a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation.
25053175	5	48	theme	atomic	1005:1010	arg1	microscope					1018:1027	atomic force microscope	1005:1027	atomic force microscope	1005:1027	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	1	49	theme	surface	249:255	arg1	construction					201:212	the construction	197:212	the construction of a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation	197:326	A novel surface-modification strategy has been developed for the construction of a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation.
25053175	5	50	theme	contact	1034:1040	arg1	measurements					1048:1059	contact angle measurements	1034:1059	contact angle measurements	1034:1059	The elemental composition, surface morphology, and hydrophilic/hydrophobic property of the modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscope, and contact angle measurements, respectively.
25053175	1	51	theme	surface-modification	144:163	arg1	strategy					165:172	A novel surface-modification strategy	136:172	A novel surface-modification strategy	136:172	A novel surface-modification strategy has been developed for the construction of a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation.
25053175	2	52	theme	guest	369:373	arg1	Azo					396:398	Azo	396:398	Azo	396:398	The silicon wafer was first modified by guest molecule azobenzene (Azo) via a silanization reaction.
25053175	2	52	theme	guest	369:373	arg1	azobenzene					384:393	guest molecule azobenzene	369:393	guest molecule azobenzene (Azo)	369:399	The silicon wafer was first modified by guest molecule azobenzene (Azo) via a silanization reaction.
25053175	7	53	theme	protein-resistant	1318:1334	arg1	properties					1336:1345	good protein-resistant properties	1313:1345	good protein-resistant properties	1313:1345	The results demonstrate that the surface modified with PEG possesses good protein-resistant properties.
25053175	3	54	theme	molecule	513:520	arg1	β-cyclodextrin					522:535	host molecule β-cyclodextrin	508:535	host molecule β-cyclodextrin (β-CD)	508:542	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	3	54	theme	molecule	513:520	arg1	β-CD					538:541	β-CD	538:541	β-CD	538:541	Subsequently, a series of polymers with different polarities were attached to host molecule β-cyclodextrin (β-CD) to prepare β-CD-containing hemitelechelic polymers via click chemistry.
25053175	1	55	theme	switchable	262:271	arg1	wettability					273:283	switchable wettability	262:283	switchable wettability based on host-guest inclusion complexation	262:326	A novel surface-modification strategy has been developed for the construction of a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation.
25053175	4	56	theme	silicon	643:649	arg1	surface					657:663	a photocontrolled silicon wafer surface	625:663	a photocontrolled silicon wafer surface modified with polymers	625:686	Finally, a photocontrolled silicon wafer surface modified with polymers was fabricated by inclusion complexation between β-CD and Azo, and the surface properties of the substrate are dependent on the polymers we used.
25053175	1	57	with	surface	249:255	arg1	wettability					273:283	switchable wettability	262:283	switchable wettability based on host-guest inclusion complexation	262:326	A novel surface-modification strategy has been developed for the construction of a photocontrolled silicon wafer surface with switchable wettability based on host-guest inclusion complexation.
25053175	4	58	dep	polymers	816:823	arg1	we					825:826	we	825:826	we	825:826	Finally, a photocontrolled silicon wafer surface modified with polymers was fabricated by inclusion complexation between β-CD and Azo, and the surface properties of the substrate are dependent on the polymers we used.
28962780	5	0	from	porosity	767:774	arg1	water					832:836	water	832:836	water	832:836	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	1	1	theme	gelatin-bacterial	191:207	arg1	sponges					234:240	gelatin-bacterial cellulose (GB) composite sponges	191:240	gelatin-bacterial cellulose (GB) composite sponges	191:240	Freeze-drying and thermal cross-linking techniques were used to prepare gelatin-bacterial cellulose (GB) composite sponges for potential application as scaffolds in tissue engineering.
28962780	0	2	theme	engineering	93:103	arg1	applications					105:116	tissue engineering applications	86:116	tissue engineering applications	86:116	Gelatin-bacterial cellulose composite sponges thermally cross-linked with glucose for tissue engineering applications.
28962780	3	3	from	effects	437:443	arg1	sponges					574:580	the GB sponges	567:580	the GB sponges	567:580	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	5	4	from	biocompatibility	861:876	arg1	water					832:836	water	832:836	water	832:836	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	1	5	theme	cellulose	209:217	arg1	sponges					234:240	gelatin-bacterial cellulose (GB) composite sponges	191:240	gelatin-bacterial cellulose (GB) composite sponges	191:240	Freeze-drying and thermal cross-linking techniques were used to prepare gelatin-bacterial cellulose (GB) composite sponges for potential application as scaffolds in tissue engineering.
28962780	2	6	theme	toxic	324:328	arg1	agents					355:360	toxic and costly cross-linking agents	324:360	toxic and costly cross-linking agents	324:360	To avoid the use of toxic and costly cross-linking agents, glucose was used to cross-link the gelatin via the Maillard reaction.
28962780	2	7	theme	agents	355:360	arg1	use					317:319	the use	313:319	the use of toxic and costly cross-linking agents	313:360	To avoid the use of toxic and costly cross-linking agents, glucose was used to cross-link the gelatin via the Maillard reaction.
28962780	5	8	theme	good	777:780	arg1	properties					791:800	good swelling properties	777:800	good swelling properties	777:800	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	4	9	theme	gelatin	686:692	arg1	ratio					704:708	a gelatin:BC weight ratio	684:708	a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h	684:755	An open and highly interconnected porous structure was attained for the GB sponge with a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h.
28962780	4	10	theme	weight	697:702	arg1	ratio					704:708	a gelatin:BC weight ratio	684:708	a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h	684:755	An open and highly interconnected porous structure was attained for the GB sponge with a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h.
28962780	5	11	theme	good	803:806	arg1	stability					819:827	good structural stability	803:827	good structural stability in water	803:836	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	3	12	theme	ratio	459:463	arg1	effects					437:443	The effects	433:443	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges	433:580	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	5	13	theme	tissue	948:953	arg1	engineering					955:965	tissue engineering	948:965	tissue engineering	948:965	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	2	14	theme	Maillard	414:421	arg1	reaction					423:430	the Maillard reaction	410:430	the Maillard reaction	410:430	To avoid the use of toxic and costly cross-linking agents, glucose was used to cross-link the gelatin via the Maillard reaction.
28962780	0	15	theme	cellulose	18:26	arg1	sponges					38:44	Gelatin-bacterial cellulose composite sponges	0:44	Gelatin-bacterial cellulose composite sponges	0:44	Gelatin-bacterial cellulose composite sponges thermally cross-linked with glucose for tissue engineering applications.
28962780	1	16	used	used	175:178	arg2	techniques					159:168	Freeze-drying and thermal cross-linking techniques	119:168	Freeze-drying and thermal cross-linking techniques	119:168	Freeze-drying and thermal cross-linking techniques were used to prepare gelatin-bacterial cellulose (GB) composite sponges for potential application as scaffolds in tissue engineering.
28962780	5	17	from	properties	791:800	arg1	water					832:836	water	832:836	water	832:836	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	0	18	theme	Gelatin-bacterial	0:16	arg1	sponges					38:44	Gelatin-bacterial cellulose composite sponges	0:44	Gelatin-bacterial cellulose composite sponges	0:44	Gelatin-bacterial cellulose composite sponges thermally cross-linked with glucose for tissue engineering applications.
28962780	2	19	theme	cross-linking	341:353	arg1	agents					355:360	toxic and costly cross-linking agents	324:360	toxic and costly cross-linking agents	324:360	To avoid the use of toxic and costly cross-linking agents, glucose was used to cross-link the gelatin via the Maillard reaction.
28962780	1	20	theme	composite	224:232	arg1	sponges					234:240	gelatin-bacterial cellulose (GB) composite sponges	191:240	gelatin-bacterial cellulose (GB) composite sponges	191:240	Freeze-drying and thermal cross-linking techniques were used to prepare gelatin-bacterial cellulose (GB) composite sponges for potential application as scaffolds in tissue engineering.
28962780	5	21	theme	structural	808:817	arg1	stability					819:827	good structural stability	803:827	good structural stability in water	803:836	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	4	22	theme	25:75	713:717	arg1	ratio					704:708	a gelatin:BC weight ratio	684:708	a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h	684:755	An open and highly interconnected porous structure was attained for the GB sponge with a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h.
28962780	2	23	used	used	375:378	arg2	glucose					363:369	glucose	363:369	glucose	363:369	To avoid the use of toxic and costly cross-linking agents, glucose was used to cross-link the gelatin via the Maillard reaction.
28962780	2	24	theme	costly	334:339	arg1	agents					355:360	toxic and costly cross-linking agents	324:360	toxic and costly cross-linking agents	324:360	To avoid the use of toxic and costly cross-linking agents, glucose was used to cross-link the gelatin via the Maillard reaction.
28962780	3	25	theme	bacterial	479:487	arg1	BC					500:501	BC	500:501	BC	500:501	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	3	25	theme	bacterial	479:487	arg1	cellulose					489:497	bacterial cellulose	479:497	bacterial cellulose (BC)	479:502	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	1	26	theme	Freeze-drying	119:131	arg1	techniques					159:168	Freeze-drying and thermal cross-linking techniques	119:168	Freeze-drying and thermal cross-linking techniques	119:168	Freeze-drying and thermal cross-linking techniques were used to prepare gelatin-bacterial cellulose (GB) composite sponges for potential application as scaffolds in tissue engineering.
28962780	1	27	theme	thermal	137:143	arg1	techniques					159:168	Freeze-drying and thermal cross-linking techniques	119:168	Freeze-drying and thermal cross-linking techniques	119:168	Freeze-drying and thermal cross-linking techniques were used to prepare gelatin-bacterial cellulose (GB) composite sponges for potential application as scaffolds in tissue engineering.
28962780	4	28	theme	porous	631:636	arg1	structure					638:646	An open and highly interconnected porous structure	597:646	An open and highly interconnected porous structure	597:646	An open and highly interconnected porous structure was attained for the GB sponge with a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h.
28962780	4	29	theme	BC	694:695	arg1	ratio					704:708	a gelatin:BC weight ratio	684:708	a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h	684:755	An open and highly interconnected porous structure was attained for the GB sponge with a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h.
28962780	3	30	theme	gelatin	468:474	arg1	ratio					459:463	the weight ratio	448:463	the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration)	448:562	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	3	31	theme	weight	452:457	arg1	ratio					459:463	the weight ratio	448:463	the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration)	448:562	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	1	32	theme	potential	246:254	arg1	application					256:266	potential application	246:266	potential application as scaffolds in tissue engineering	246:301	Freeze-drying and thermal cross-linking techniques were used to prepare gelatin-bacterial cellulose (GB) composite sponges for potential application as scaffolds in tissue engineering.
28962780	5	33	theme	swelling	782:789	arg1	properties					791:800	good swelling properties	777:800	good swelling properties	777:800	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	4	34	attach	cross-linked	728:739	arg3	140°C					744:748	140°C	744:748	140°C for 3h	744:755	An open and highly interconnected porous structure was attained for the GB sponge with a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h.
28962780	5	35	theme	high	762:765	arg1	porosity					767:774	Its high porosity	758:774	Its high porosity	758:774	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	5	36	from	stability	819:827	arg1	water					832:836	water	832:836	water	832:836	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	1	37	theme	cross-linking	145:157	arg1	techniques					159:168	Freeze-drying and thermal cross-linking techniques	119:168	Freeze-drying and thermal cross-linking techniques	119:168	Freeze-drying and thermal cross-linking techniques were used to prepare gelatin-bacterial cellulose (GB) composite sponges for potential application as scaffolds in tissue engineering.
28962780	3	38	theme	cross-linking	512:524	arg1	duration					554:561	duration	554:561	duration	554:561	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	3	38	theme	cross-linking	512:524	arg1	temperature					538:548	temperature	538:548	temperature	538:548	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	3	38	theme	cross-linking	512:524	arg1	conditions					526:535	the cross-linking conditions	508:535	the cross-linking conditions (temperature and duration)	508:562	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	4	39	theme	GB	669:670	arg1	sponge					672:677	the GB sponge	665:677	the GB sponge	665:677	An open and highly interconnected porous structure was attained for the GB sponge with a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h.
28962780	3	40	theme	GB	571:572	arg1	sponges					574:580	the GB sponges	567:580	the GB sponges	567:580	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	3	41	dep	conditions	526:535	arg1	duration					554:561	duration	554:561	duration	554:561	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	3	41	dep	conditions	526:535	arg1	temperature					538:548	temperature	538:548	temperature	538:548	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	3	41	dep	conditions	526:535	arg1	conditions					526:535	the cross-linking conditions	508:535	the cross-linking conditions (temperature and duration)	508:562	The effects of the weight ratio of gelatin to bacterial cellulose (BC) and the cross-linking conditions (temperature and duration) on the GB sponges were examined.
28962780	1	42	from	scaffolds	271:279	arg1	engineering					291:301	tissue engineering	284:301	tissue engineering	284:301	Freeze-drying and thermal cross-linking techniques were used to prepare gelatin-bacterial cellulose (GB) composite sponges for potential application as scaffolds in tissue engineering.
28962780	5	43	theme	Vero	886:889	arg1	cell					891:894	Vero cell	886:894	Vero cell	886:894	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	4	44	theme	open	600:603	arg1	structure					638:646	An open and highly interconnected porous structure	597:646	An open and highly interconnected porous structure	597:646	An open and highly interconnected porous structure was attained for the GB sponge with a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h.
28962780	0	45	theme	tissue	86:91	arg1	applications					105:116	tissue engineering applications	86:116	tissue engineering applications	86:116	Gelatin-bacterial cellulose composite sponges thermally cross-linked with glucose for tissue engineering applications.
28962780	5	46	from	non-toxicity	839:850	arg1	water					832:836	water	832:836	water	832:836	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	1	47	theme	GB	220:221	arg1	sponges					234:240	gelatin-bacterial cellulose (GB) composite sponges	191:240	gelatin-bacterial cellulose (GB) composite sponges	191:240	Freeze-drying and thermal cross-linking techniques were used to prepare gelatin-bacterial cellulose (GB) composite sponges for potential application as scaffolds in tissue engineering.
28962780	4	48	theme	interconnected	616:629	arg1	structure					638:646	An open and highly interconnected porous structure	597:646	An open and highly interconnected porous structure	597:646	An open and highly interconnected porous structure was attained for the GB sponge with a gelatin:BC weight ratio of 25:75 that was cross-linked at 140°C for 3h.
28962780	0	49	theme	composite	28:36	arg1	sponges					38:44	Gelatin-bacterial cellulose composite sponges	0:44	Gelatin-bacterial cellulose composite sponges	0:44	Gelatin-bacterial cellulose composite sponges thermally cross-linked with glucose for tissue engineering applications.
28962780	5	50	theme	good	856:859	arg1	biocompatibility					861:876	good biocompatibility	856:876	good biocompatibility	856:876	Its high porosity, good swelling properties, good structural stability in water, non-toxicity and good biocompatibility against Vero cell are promising for its application as a scaffold for tissue engineering.
28962780	1	51	theme	tissue	284:289	arg1	engineering					291:301	tissue engineering	284:301	tissue engineering	284:301	Freeze-drying and thermal cross-linking techniques were used to prepare gelatin-bacterial cellulose (GB) composite sponges for potential application as scaffolds in tissue engineering.
26948011	9	0	theme	anoxic	1606:1611	arg1	seeds					1613:1617	anoxic seeds	1606:1617	anoxic seeds	1606:1617	The activity of LDH and ADH was the highest in anoxic seeds.
26948011	11	1	from	growth	1887:1892	arg1	conditions					1903:1912	space conditions	1897:1912	space conditions	1897:1912	Therefore, sufficient oxygen availability is important for germination before photosynthesis provides necessary oxygen and the determination of an oxygen carrying capacity is important for uniform growth in space conditions.
26948011	1	2	theme	reduced	145:151	arg1	pressure					153:160	reduced pressure	145:160	reduced pressure	145:160	Establishing plants in space, Moon or Mars requires adaptation to altered conditions, including reduced pressure and composition of atmospheres.
26948011	4	3	theme	lower	726:730	arg1	pO2					732:734	lower pO2	726:734	lower pO2	726:734	Partial oxygen pressure (pO2) greater than 10% resulted in normal germination (i.e., protrusion of radicle about 18 hours after imbibition) but lower pO2 delayed and reduced germination.
26948011	5	4	theme	air	878:880	arg1	germination					892:902	air initiated germination	878:902	air initiated germination	878:902	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	8	5	theme	1,6-bisphosphate	1407:1422	arg1	ALD					1434:1436	ALD	1434:1436	ALD	1434:1436	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	5	theme	1,6-bisphosphate	1407:1422	arg1	genes					1327:1331	fermentation pathway-related genes	1298:1331	fermentation pathway-related genes	1298:1331	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	5	theme	1,6-bisphosphate	1407:1422	arg1	aldolase					1424:1431	fructose 1,6-bisphosphate aldolase	1398:1431	fructose 1,6-bisphosphate aldolase (ALD)	1398:1437	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	5	6	theme	subsequent	855:864	arg1	transfer					866:873	subsequent transfer	855:873	subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate	855:947	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	7	7	theme	starch	1171:1176	arg1	degradation					1178:1188	starch degradation	1171:1188	starch degradation	1171:1188	Transcription of genes related to starch degradation, α-amylase (AMY) and Sucrose Synthase (SUS), was higher under ambient O2 than under hypoxia.
26948011	6	8	theme	soluble	1034:1040	arg1	sugars					1042:1047	soluble sugars	1034:1047	soluble sugars	1034:1047	In hypoxic seeds soluble sugars degraded faster but the content of starch after 24 h was higher than at ambient oxygen.
26948011	8	9	theme	fructose	1398:1405	arg1	ALD					1434:1436	ALD	1434:1436	ALD	1434:1436	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	9	theme	fructose	1398:1405	arg1	genes					1327:1331	fermentation pathway-related genes	1298:1331	fermentation pathway-related genes	1298:1331	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	9	theme	fructose	1398:1405	arg1	aldolase					1424:1431	fructose 1,6-bisphosphate aldolase	1398:1431	fructose 1,6-bisphosphate aldolase (ALD)	1398:1437	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	5	10	from	transfer	866:873	arg1	%					909:909	75%	907:909	75% of the seeds	907:922	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	5	10	from	transfer	866:873	arg1	rate					944:947	the root growth rate	928:947	the root growth rate	928:947	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	5	10	from	transfer	866:873	arg1	seeds					918:922	the seeds	914:922	the seeds	914:922	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	11	11	theme	space	1897:1901	arg1	conditions					1903:1912	space conditions	1897:1912	space conditions	1897:1912	Therefore, sufficient oxygen availability is important for germination before photosynthesis provides necessary oxygen and the determination of an oxygen carrying capacity is important for uniform growth in space conditions.
26948011	4	12	dep	protrusion	667:676	arg1	i.e.					661:664	i.e.	661:664	i.e.	661:664	Partial oxygen pressure (pO2) greater than 10% resulted in normal germination (i.e., protrusion of radicle about 18 hours after imbibition) but lower pO2 delayed and reduced germination.
26948011	4	13	theme	Partial	582:588	arg1	pressure					597:604	Partial oxygen pressure	582:604	Partial oxygen pressure (pO2) greater than 10%	582:627	Partial oxygen pressure (pO2) greater than 10% resulted in normal germination (i.e., protrusion of radicle about 18 hours after imbibition) but lower pO2 delayed and reduced germination.
26948011	4	13	theme	Partial	582:588	arg1	pO2					607:609	pO2	607:609	pO2	607:609	Partial oxygen pressure (pO2) greater than 10% resulted in normal germination (i.e., protrusion of radicle about 18 hours after imbibition) but lower pO2 delayed and reduced germination.
26948011	5	14	theme	seeds	918:922	arg1	%					909:909	75%	907:909	75% of the seeds	907:922	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	5	14	theme	seeds	918:922	arg1	rate					944:947	the root growth rate	928:947	the root growth rate	928:947	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	5	14	theme	seeds	918:922	arg1	seeds					918:922	the seeds	914:922	the seeds	914:922	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	2	15	theme	genes	363:367	arg1	patterns					351:358	the transcription patterns	333:358	the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation	333:452	To determine the oxygen requirements for seed germination, we imbibed Brassica rapa seeds under varying oxygen concentrations and profiled the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation.
26948011	11	16	theme	oxygen	1712:1717	arg1	availability					1719:1730	sufficient oxygen availability	1701:1730	sufficient oxygen availability	1701:1730	Therefore, sufficient oxygen availability is important for germination before photosynthesis provides necessary oxygen and the determination of an oxygen carrying capacity is important for uniform growth in space conditions.
26948011	2	17	theme	rapa	273:276	arg1	seeds					278:282	Brassica rapa seeds	264:282	Brassica rapa seeds	264:282	To determine the oxygen requirements for seed germination, we imbibed Brassica rapa seeds under varying oxygen concentrations and profiled the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation.
26948011	5	18	theme	initiated	882:890	arg1	germination					892:902	air initiated germination	878:902	air initiated germination	878:902	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	6	19	theme	hypoxic	1020:1026	arg1	seeds					1028:1032	hypoxic seeds	1020:1032	hypoxic seeds	1020:1032	In hypoxic seeds soluble sugars degraded faster but the content of starch after 24 h was higher than at ambient oxygen.
26948011	6	20	theme	starch	1084:1089	arg1	higher					1106:1111	higher	1106:1111	higher	1106:1111	In hypoxic seeds soluble sugars degraded faster but the content of starch after 24 h was higher than at ambient oxygen.
26948011	6	20	theme	starch	1084:1089	arg1	content					1073:1079	the content	1069:1079	the content of starch after 24 h	1069:1100	In hypoxic seeds soluble sugars degraded faster but the content of starch after 24 h was higher than at ambient oxygen.
26948011	2	21	theme	Brassica	264:271	arg1	seeds					278:282	Brassica rapa seeds	264:282	Brassica rapa seeds	264:282	To determine the oxygen requirements for seed germination, we imbibed Brassica rapa seeds under varying oxygen concentrations and profiled the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation.
26948011	5	22	from	Imbibition	769:778	arg1	atmosphere					798:807	an oxygen-free atmosphere	783:807	an oxygen-free atmosphere	783:807	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	11	23	theme	sufficient	1701:1710	arg1	availability					1719:1730	sufficient oxygen availability	1701:1730	sufficient oxygen availability	1701:1730	Therefore, sufficient oxygen availability is important for germination before photosynthesis provides necessary oxygen and the determination of an oxygen carrying capacity is important for uniform growth in space conditions.
26948011	10	24	theme	low	1638:1640	arg1	conditions					1645:1654	low O2 conditions	1638:1654	low O2 conditions	1638:1654	Germination under low O2 conditions initiated ethanolic fermentation.
26948011	4	25	theme	normal	641:646	arg1	germination					648:658	normal germination	641:658	normal germination	641:658	Partial oxygen pressure (pO2) greater than 10% resulted in normal germination (i.e., protrusion of radicle about 18 hours after imbibition) but lower pO2 delayed and reduced germination.
26948011	0	26	theme	Oxygen	0:5	arg1	dependency					7:16	Oxygen dependency	0:16	Oxygen dependency of germinating Brassica seeds.	0:47	Oxygen dependency of germinating Brassica seeds.
26948011	8	27	theme	glyceraldehyde-3-phosphate	1440:1465	arg1	dehydrogenase					1467:1479	glyceraldehyde-3-phosphate dehydrogenase	1440:1479	glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	1440:1487	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	27	theme	glyceraldehyde-3-phosphate	1440:1465	arg1	genes					1327:1331	fermentation pathway-related genes	1298:1331	fermentation pathway-related genes	1298:1331	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	27	theme	glyceraldehyde-3-phosphate	1440:1465	arg1	GAPDH					1482:1486	GAPDH	1482:1486	GAPDH	1482:1486	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	2	28	theme	early	380:384	arg1	metabolism					386:395	early metabolism	380:395	early metabolism such as starch degradation, glycolysis, and fermentation	380:452	To determine the oxygen requirements for seed germination, we imbibed Brassica rapa seeds under varying oxygen concentrations and profiled the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation.
26948011	0	29	theme	germinating	21:31	arg1	seeds					42:46	germinating Brassica seeds	21:46	germinating Brassica seeds	21:46	Oxygen dependency of germinating Brassica seeds.
26948011	6	30	theme	ambient	1121:1127	arg1	oxygen					1129:1134	ambient oxygen	1121:1134	ambient oxygen	1121:1134	In hypoxic seeds soluble sugars degraded faster but the content of starch after 24 h was higher than at ambient oxygen.
26948011	11	31	theme	oxygen	1837:1842	arg1	important					1865:1873	important	1865:1873	important	1865:1873	Therefore, sufficient oxygen availability is important for germination before photosynthesis provides necessary oxygen and the determination of an oxygen carrying capacity is important for uniform growth in space conditions.
26948011	11	31	theme	oxygen	1837:1842	arg1	determination					1817:1829	the determination	1813:1829	the determination of an oxygen carrying capacity	1813:1860	Therefore, sufficient oxygen availability is important for germination before photosynthesis provides necessary oxygen and the determination of an oxygen carrying capacity is important for uniform growth in space conditions.
26948011	8	32	theme	fermentation	1298:1309	arg1	isomerase					1352:1360	glucose phosphate isomerase	1334:1360	glucose phosphate isomerase (GPI)	1334:1366	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	32	theme	fermentation	1298:1309	arg1	ADH					1529:1531	ADH	1529:1531	ADH	1529:1531	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	32	theme	fermentation	1298:1309	arg1	genes					1327:1331	fermentation pathway-related genes	1298:1331	fermentation pathway-related genes	1298:1331	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	32	theme	fermentation	1298:1309	arg1	LDH					1520:1522	LDH	1520:1522	LDH	1520:1522	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	32	theme	fermentation	1298:1309	arg1	dehydrogenase					1467:1479	glyceraldehyde-3-phosphate dehydrogenase	1440:1479	glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	1440:1487	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	32	theme	fermentation	1298:1309	arg1	6-phosphofructokinase					1369:1389	6-phosphofructokinase	1369:1389	6-phosphofructokinase (PFK)	1369:1395	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	32	theme	fermentation	1298:1309	arg1	decarboxylase					1499:1511	pyruvate decarboxylase	1490:1511	pyruvate decarboxylase (PDC)	1490:1517	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	32	theme	fermentation	1298:1309	arg1	aldolase					1424:1431	fructose 1,6-bisphosphate aldolase	1398:1431	fructose 1,6-bisphosphate aldolase (ALD)	1398:1437	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	2	33	theme	oxygen	298:303	arg1	concentrations					305:318	varying oxygen concentrations	290:318	varying oxygen concentrations	290:318	To determine the oxygen requirements for seed germination, we imbibed Brassica rapa seeds under varying oxygen concentrations and profiled the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation.
26948011	8	34	theme	pathway-related	1311:1325	arg1	isomerase					1352:1360	glucose phosphate isomerase	1334:1360	glucose phosphate isomerase (GPI)	1334:1366	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	34	theme	pathway-related	1311:1325	arg1	ADH					1529:1531	ADH	1529:1531	ADH	1529:1531	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	34	theme	pathway-related	1311:1325	arg1	genes					1327:1331	fermentation pathway-related genes	1298:1331	fermentation pathway-related genes	1298:1331	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	34	theme	pathway-related	1311:1325	arg1	LDH					1520:1522	LDH	1520:1522	LDH	1520:1522	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	34	theme	pathway-related	1311:1325	arg1	dehydrogenase					1467:1479	glyceraldehyde-3-phosphate dehydrogenase	1440:1479	glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	1440:1487	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	34	theme	pathway-related	1311:1325	arg1	6-phosphofructokinase					1369:1389	6-phosphofructokinase	1369:1389	6-phosphofructokinase (PFK)	1369:1395	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	34	theme	pathway-related	1311:1325	arg1	decarboxylase					1499:1511	pyruvate decarboxylase	1490:1511	pyruvate decarboxylase (PDC)	1490:1517	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	34	theme	pathway-related	1311:1325	arg1	aldolase					1424:1431	fructose 1,6-bisphosphate aldolase	1398:1431	fructose 1,6-bisphosphate aldolase (ALD)	1398:1437	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	5	35	theme	oxygen-free	786:796	arg1	atmosphere					798:807	an oxygen-free atmosphere	783:807	an oxygen-free atmosphere	783:807	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	7	36	theme	Sucrose	1211:1217	arg1	Synthase					1219:1226	Sucrose Synthase	1211:1226	Sucrose Synthase (SUS)	1211:1232	Transcription of genes related to starch degradation, α-amylase (AMY) and Sucrose Synthase (SUS), was higher under ambient O2 than under hypoxia.
26948011	7	36	theme	Sucrose	1211:1217	arg1	genes					1154:1158	genes	1154:1158	genes related to starch degradation	1154:1188	Transcription of genes related to starch degradation, α-amylase (AMY) and Sucrose Synthase (SUS), was higher under ambient O2 than under hypoxia.
26948011	7	36	theme	Sucrose	1211:1217	arg1	SUS					1229:1231	SUS	1229:1231	SUS	1229:1231	Transcription of genes related to starch degradation, α-amylase (AMY) and Sucrose Synthase (SUS), was higher under ambient O2 than under hypoxia.
26948011	3	37	theme	alcohol	520:526	arg1	ADH					543:545	ADH	543:545	ADH	543:545	We also analyzed the activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH), and measured starch degradation.
26948011	3	37	theme	alcohol	520:526	arg1	dehydrogenase					528:540	alcohol dehydrogenase	520:540	alcohol dehydrogenase (ADH)	520:546	We also analyzed the activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH), and measured starch degradation.
26948011	2	38	theme	varying	290:296	arg1	concentrations					305:318	varying oxygen concentrations	290:318	varying oxygen concentrations	290:318	To determine the oxygen requirements for seed germination, we imbibed Brassica rapa seeds under varying oxygen concentrations and profiled the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation.
26948011	8	39	theme	phosphate	1342:1350	arg1	GPI					1363:1365	GPI	1363:1365	GPI	1363:1365	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	39	theme	phosphate	1342:1350	arg1	isomerase					1352:1360	glucose phosphate isomerase	1334:1360	glucose phosphate isomerase (GPI)	1334:1366	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	39	theme	phosphate	1342:1350	arg1	genes					1327:1331	fermentation pathway-related genes	1298:1331	fermentation pathway-related genes	1298:1331	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	0	40	theme	seeds	42:46	arg1	dependency					7:16	Oxygen dependency	0:16	Oxygen dependency of germinating Brassica seeds.	0:47	Oxygen dependency of germinating Brassica seeds.
26948011	3	41	theme	dehydrogenase	496:508	arg1	activity					476:483	the activity	472:483	the activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH)	472:546	We also analyzed the activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH), and measured starch degradation.
26948011	2	42	theme	seed	235:238	arg1	germination					240:250	seed germination	235:250	seed germination	235:250	To determine the oxygen requirements for seed germination, we imbibed Brassica rapa seeds under varying oxygen concentrations and profiled the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation.
26948011	1	43	theme	atmospheres	181:191	arg1	composition					166:176	composition	166:176	composition	166:176	Establishing plants in space, Moon or Mars requires adaptation to altered conditions, including reduced pressure and composition of atmospheres.
26948011	1	43	theme	atmospheres	181:191	arg1	pressure					153:160	reduced pressure	145:160	reduced pressure	145:160	Establishing plants in space, Moon or Mars requires adaptation to altered conditions, including reduced pressure and composition of atmospheres.
26948011	0	44	theme	Brassica	33:40	arg1	seeds					42:46	germinating Brassica seeds	21:46	germinating Brassica seeds	21:46	Oxygen dependency of germinating Brassica seeds.
26948011	9	45	theme	LDH	1575:1577	arg1	highest					1595:1601	highest	1595:1601	highest	1595:1601	The activity of LDH and ADH was the highest in anoxic seeds.
26948011	9	45	theme	LDH	1575:1577	arg1	activity					1563:1570	The activity	1559:1570	The activity of LDH and ADH	1559:1585	The activity of LDH and ADH was the highest in anoxic seeds.
26948011	2	46	theme	starch	405:410	arg1	degradation					412:422	starch degradation	405:422	starch degradation	405:422	To determine the oxygen requirements for seed germination, we imbibed Brassica rapa seeds under varying oxygen concentrations and profiled the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation.
26948011	3	47	theme	starch	562:567	arg1	degradation					569:579	starch degradation	562:579	starch degradation	562:579	We also analyzed the activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH), and measured starch degradation.
26948011	3	48	theme	lactate	488:494	arg1	LDH					511:513	LDH	511:513	LDH	511:513	We also analyzed the activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH), and measured starch degradation.
26948011	3	48	theme	lactate	488:494	arg1	dehydrogenase					496:508	lactate dehydrogenase	488:508	lactate dehydrogenase (LDH)	488:514	We also analyzed the activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH), and measured starch degradation.
26948011	11	49	contain	carrying	1844:1851	arg2	capacity					1853:1860	capacity	1853:1860	capacity	1853:1860	Therefore, sufficient oxygen availability is important for germination before photosynthesis provides necessary oxygen and the determination of an oxygen carrying capacity is important for uniform growth in space conditions.
26948011	11	49	contain	carrying	1844:1851	arg1	oxygen					1837:1842	an oxygen	1834:1842	an oxygen carrying capacity	1834:1860	Therefore, sufficient oxygen availability is important for germination before photosynthesis provides necessary oxygen and the determination of an oxygen carrying capacity is important for uniform growth in space conditions.
26948011	8	50	theme	pyruvate	1490:1497	arg1	PDC					1514:1516	PDC	1514:1516	PDC	1514:1516	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	50	theme	pyruvate	1490:1497	arg1	decarboxylase					1499:1511	pyruvate decarboxylase	1490:1511	pyruvate decarboxylase (PDC)	1490:1517	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	50	theme	pyruvate	1490:1497	arg1	genes					1327:1331	fermentation pathway-related genes	1298:1331	fermentation pathway-related genes	1298:1331	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	9	51	theme	ADH	1583:1585	arg1	highest					1595:1601	highest	1595:1601	highest	1595:1601	The activity of LDH and ADH was the highest in anoxic seeds.
26948011	9	51	theme	ADH	1583:1585	arg1	activity					1563:1570	The activity	1559:1570	The activity of LDH and ADH	1559:1585	The activity of LDH and ADH was the highest in anoxic seeds.
26948011	2	52	theme	oxygen	211:216	arg1	requirements					218:229	the oxygen requirements	207:229	the oxygen requirements for seed germination	207:250	To determine the oxygen requirements for seed germination, we imbibed Brassica rapa seeds under varying oxygen concentrations and profiled the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation.
26948011	2	53	theme	transcription	337:349	arg1	patterns					351:358	the transcription patterns	333:358	the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation	333:452	To determine the oxygen requirements for seed germination, we imbibed Brassica rapa seeds under varying oxygen concentrations and profiled the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation.
26948011	8	54	theme	glucose	1334:1340	arg1	GPI					1363:1365	GPI	1363:1365	GPI	1363:1365	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	54	theme	glucose	1334:1340	arg1	isomerase					1352:1360	glucose phosphate isomerase	1334:1360	glucose phosphate isomerase (GPI)	1334:1366	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	8	54	theme	glucose	1334:1340	arg1	genes					1327:1331	fermentation pathway-related genes	1298:1331	fermentation pathway-related genes	1298:1331	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
26948011	5	55	theme	root	932:935	arg1	rate					944:947	the root growth rate	928:947	the root growth rate	928:947	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	1	56	theme	altered	115:121	arg1	conditions					123:132	altered conditions	115:132	altered conditions	115:132	Establishing plants in space, Moon or Mars requires adaptation to altered conditions, including reduced pressure and composition of atmospheres.
26948011	5	57	theme	no	836:837	arg1	germination					839:849	no germination	836:849	no germination	836:849	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	3	58	theme	dehydrogenase	528:540	arg1	activity					476:483	the activity	472:483	the activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH)	472:546	We also analyzed the activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH), and measured starch degradation.
26948011	4	59	dep	pressure	597:604	arg1	%					627:627	%	627:627	%	627:627	Partial oxygen pressure (pO2) greater than 10% resulted in normal germination (i.e., protrusion of radicle about 18 hours after imbibition) but lower pO2 delayed and reduced germination.
26948011	5	60	theme	growth	937:942	arg1	rate					944:947	the root growth rate	928:947	the root growth rate	928:947	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	4	61	theme	radicle	681:687	arg1	protrusion					667:676	protrusion	667:676	protrusion of radicle about 18 hours	667:702	Partial oxygen pressure (pO2) greater than 10% resulted in normal germination (i.e., protrusion of radicle about 18 hours after imbibition) but lower pO2 delayed and reduced germination.
26948011	4	62	theme	oxygen	590:595	arg1	pressure					597:604	Partial oxygen pressure	582:604	Partial oxygen pressure (pO2) greater than 10%	582:627	Partial oxygen pressure (pO2) greater than 10% resulted in normal germination (i.e., protrusion of radicle about 18 hours after imbibition) but lower pO2 delayed and reduced germination.
26948011	4	62	theme	oxygen	590:595	arg1	pO2					607:609	pO2	607:609	pO2	607:609	Partial oxygen pressure (pO2) greater than 10% resulted in normal germination (i.e., protrusion of radicle about 18 hours after imbibition) but lower pO2 delayed and reduced germination.
26948011	7	63	theme	genes	1154:1158	arg1	Transcription					1137:1149	Transcription	1137:1149	Transcription of genes related to starch degradation, α-amylase (AMY) and Sucrose Synthase (SUS),	1137:1233	Transcription of genes related to starch degradation, α-amylase (AMY) and Sucrose Synthase (SUS), was higher under ambient O2 than under hypoxia.
26948011	5	64	theme	ambient	1004:1010	arg1	pO2					1012:1014	ambient pO2	1004:1014	ambient pO2	1004:1014	Imbibition in an oxygen-free atmosphere for three days resulted in no germination but subsequent transfer to air initiated germination in 75% of the seeds and the root growth rate was transiently greater than in roots germinated under ambient pO2.
26948011	4	65	dep	resulted	629:636	arg1	protrusion					667:676	protrusion	667:676	protrusion of radicle about 18 hours	667:702	Partial oxygen pressure (pO2) greater than 10% resulted in normal germination (i.e., protrusion of radicle about 18 hours after imbibition) but lower pO2 delayed and reduced germination.
26948011	11	66	theme	uniform	1879:1885	arg1	growth					1887:1892	uniform growth	1879:1892	uniform growth in space conditions	1879:1912	Therefore, sufficient oxygen availability is important for germination before photosynthesis provides necessary oxygen and the determination of an oxygen carrying capacity is important for uniform growth in space conditions.
26948011	11	67	theme	necessary	1792:1800	arg1	oxygen					1802:1807	necessary oxygen	1792:1807	necessary oxygen	1792:1807	Therefore, sufficient oxygen availability is important for germination before photosynthesis provides necessary oxygen and the determination of an oxygen carrying capacity is important for uniform growth in space conditions.
26948011	7	68	theme	related	1160:1166	arg1	Synthase					1219:1226	Sucrose Synthase	1211:1226	Sucrose Synthase (SUS)	1211:1232	Transcription of genes related to starch degradation, α-amylase (AMY) and Sucrose Synthase (SUS), was higher under ambient O2 than under hypoxia.
26948011	7	68	theme	related	1160:1166	arg1	genes					1154:1158	genes	1154:1158	genes related to starch degradation	1154:1188	Transcription of genes related to starch degradation, α-amylase (AMY) and Sucrose Synthase (SUS), was higher under ambient O2 than under hypoxia.
26948011	7	68	theme	related	1160:1166	arg1	α-amylase					1191:1199	α-amylase	1191:1199	α-amylase (AMY)	1191:1205	Transcription of genes related to starch degradation, α-amylase (AMY) and Sucrose Synthase (SUS), was higher under ambient O2 than under hypoxia.
26948011	4	69	dep	%	627:627	arg1	10					625:626	10	625:626	10	625:626	Partial oxygen pressure (pO2) greater than 10% resulted in normal germination (i.e., protrusion of radicle about 18 hours after imbibition) but lower pO2 delayed and reduced germination.
26948011	10	70	theme	ethanolic	1666:1674	arg1	fermentation					1676:1687	ethanolic fermentation	1666:1687	ethanolic fermentation	1666:1687	Germination under low O2 conditions initiated ethanolic fermentation.
26948011	2	71	theme	related	369:375	arg1	genes					363:367	genes	363:367	genes related to early metabolism such as starch degradation, glycolysis, and fermentation	363:452	To determine the oxygen requirements for seed germination, we imbibed Brassica rapa seeds under varying oxygen concentrations and profiled the transcription patterns of genes related to early metabolism such as starch degradation, glycolysis, and fermentation.
26948011	8	72	theme	low	1550:1552	arg1	pO2					1554:1556	low pO2	1550:1556	low pO2	1550:1556	Glycolysis and fermentation pathway-related genes, glucose phosphate isomerase (GPI), 6-phosphofructokinase (PFK), fructose 1,6-bisphosphate aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate decarboxylase (PDC), LDH, and ADH, were induced by low pO2.
29279118	0	0	theme	on-demand	81:89	arg1	regeneration					104:115	on-demand cranial bone regeneration	81:115	on-demand cranial bone regeneration	81:115	In situ controlled release of stromal cell-derived factor-1α and antimiR-138 for on-demand cranial bone regeneration.
29279118	4	1	theme	MSCs	958:961	arg1	differentiation					939:953	the osteogenic differentiation	924:953	the osteogenic differentiation of MSCs	924:961	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	7	2	theme	composite	1571:1579	arg1	formation					1545:1553	the in situ formation	1533:1553	the in situ formation of NPs/hydrogel composite	1533:1579	Our research indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration.
29279118	6	3	theme	osteocalcin	1354:1364	arg1	proteins					1372:1379	collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins	1307:1379	collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins	1307:1379	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	2	4	theme	regeneration	373:384	arg1	demand					358:363	the demand	354:363	the demand of bone regeneration	354:384	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	6	5	dep	group	1416:1420	arg1	SDF-1α					1441:1446	SDF-1α	1441:1446	SDF-1α	1441:1446	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	6	5	dep	group	1416:1420	arg1	NPs					1468:1470	incorporated both SDF-1α and CTH/antimiR-138 NPs	1423:1470	NPs	1468:1470	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	6	5	dep	group	1416:1420	arg1	incorporated					1423:1434	incorporated	1423:1434	incorporated	1423:1434	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	0	6	theme	bone	99:102	arg1	regeneration					104:115	on-demand cranial bone regeneration	81:115	on-demand cranial bone regeneration	81:115	In situ controlled release of stromal cell-derived factor-1α and antimiR-138 for on-demand cranial bone regeneration.
29279118	4	7	theme	sustained	842:850	arg1	release					852:858	the sustained release	838:858	the sustained release of antimiR-138 from the nanoparticle/hydrogel compound	838:913	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	6	8	dep	incorporated	1423:1434	arg1	both					1436:1439	both	1436:1439	both	1436:1439	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	3	9	theme	acid/antimiRNA-138	577:594	arg1	NPs					627:629	CTH/antimiR-138 NPs	611:629	CTH/antimiR-138 NPs	611:629	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	3	9	theme	acid/antimiRNA-138	577:594	arg1	nanoparticles					596:608	chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles	540:608	chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs)	540:630	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	3	10	theme	chitosan/β-sodium	635:651	arg1	hydrogel					680:687	chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel	635:687	chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration	635:737	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	0	11	theme	cranial	91:97	arg1	regeneration					104:115	on-demand cranial bone regeneration	81:115	on-demand cranial bone regeneration	81:115	In situ controlled release of stromal cell-derived factor-1α and antimiR-138 for on-demand cranial bone regeneration.
29279118	3	12	link	derived	509:515	arg1	SDF-1α					528:533	SDF-1α	528:533	SDF-1α	528:533	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	3	12	link	derived	509:515	arg1	factor-1α					517:525	stromal cell derived factor-1α	496:525	stromal cell derived factor-1α (SDF-1α)	496:534	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	4	13	theme	cells	810:814	arg1	migration					780:788	the migration	776:788	the migration of mesenchymal stem cells (MSCs) for 6 d	776:829	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	4	14	theme	microcomputed	1035:1047	arg1	analysis					1081:1088	histological analysis	1068:1088	histological analysis	1068:1088	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	4	14	theme	microcomputed	1035:1047	arg1	μ-CT					1061:1064	μ-CT	1061:1064	μ-CT	1061:1064	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	4	14	theme	microcomputed	1035:1047	arg1	immunohistochemistry					1094:1113	immunohistochemistry	1094:1113	immunohistochemistry	1094:1113	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	4	14	theme	microcomputed	1035:1047	arg1	tomography					1049:1058	microcomputed tomography	1035:1058	microcomputed tomography (μ-CT)	1035:1065	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	2	15	theme	bone	368:371	arg1	regeneration					373:384	bone regeneration	368:384	bone regeneration	368:384	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	6	16	theme	proteins	1372:1379	arg1	expression					1293:1302	the expression	1289:1302	the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins	1289:1379	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	3	17	theme	derived	509:515	arg1	SDF-1α					528:533	SDF-1α	528:533	SDF-1α	528:533	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	3	17	theme	derived	509:515	arg1	factor-1α					517:525	stromal cell derived factor-1α	496:525	stromal cell derived factor-1α (SDF-1α)	496:534	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	2	18	theme	factors	304:310	arg1	release					274:280	In situ controlled release	255:280	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration	255:384	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	2	18	theme	factors	304:310	arg1	strategy					399:406	a desired strategy	389:406	a desired strategy	389:406	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	6	19	theme	drug	1411:1414	arg1	group					1416:1420	the combined drug group	1398:1420	the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs)	1398:1471	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	3	20	theme	nanoparticle/hydrogel	424:444	arg1	system					456:461	nanoparticle/hydrogel composite system	424:461	nanoparticle/hydrogel composite system	424:461	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	2	21	theme	bioactive	294:302	arg1	factors					304:310	combined bioactive factors	285:310	combined bioactive factors	285:310	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	4	22	theme	fast	744:747	arg1	release					749:755	The fast release	740:755	The fast release of SDF-1α	740:765	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	5	23	theme	CTH/antimiR-138	1205:1219	arg1	NPs					1221:1223	SDF-1α and/or CTH/antimiR-138 NPs	1191:1223	NPs	1221:1223	Comparing with blank group and hydrogel group, hydrogels incorporated with SDF-1α and/or CTH/antimiR-138 NPs significantly enhanced bone regeneration (p<0.05).
29279118	6	24	theme	osteopontin	1332:1342	arg1	proteins					1372:1379	collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins	1307:1379	collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins	1307:1379	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	0	25	link	cell-derived	38:49	arg1	factor-1α					51:59	stromal cell-derived factor-1α	30:59	stromal cell-derived factor-1α	30:59	In situ controlled release of stromal cell-derived factor-1α and antimiR-138 for on-demand cranial bone regeneration.
29279118	3	26	theme	glycerol	653:660	arg1	hydrogel					680:687	chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel	635:687	chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration	635:737	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	5	27	theme	SDF-1α	1191:1196	arg1	NPs					1221:1223	SDF-1α and/or CTH/antimiR-138 NPs	1191:1223	NPs	1221:1223	Comparing with blank group and hydrogel group, hydrogels incorporated with SDF-1α and/or CTH/antimiR-138 NPs significantly enhanced bone regeneration (p<0.05).
29279118	4	28	theme	SDF-1α	760:765	arg1	release					749:755	The fast release	740:755	The fast release of SDF-1α	740:765	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	6	29	theme	hydrogel	1494:1501	arg1	group					1503:1507	the hydrogel group	1490:1507	the hydrogel group	1490:1507	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	5	30	dep	enhanced	1239:1246	arg1	p<0.05					1267:1272	p<0.05	1267:1272	p<0.05	1267:1272	Comparing with blank group and hydrogel group, hydrogels incorporated with SDF-1α and/or CTH/antimiR-138 NPs significantly enhanced bone regeneration (p<0.05).
29279118	4	31	theme	osteogenic	928:937	arg1	differentiation					939:953	the osteogenic differentiation	924:953	the osteogenic differentiation of MSCs	924:961	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	7	32	theme	cranial	1685:1691	arg1	regeneration					1698:1709	cranial bone regeneration	1685:1709	cranial bone regeneration	1685:1709	Our research indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration.
29279118	3	33	theme	phosphate	662:670	arg1	hydrogel					680:687	chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel	635:687	chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration	635:737	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	2	34	from	release	274:280	arg1	sequence					332:339	a spatiotemporal sequence	315:339	a spatiotemporal sequence for adapting the demand of bone regeneration	315:384	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	7	35	dep	in	1537:1538	arg1	situ					1540:1543	situ	1540:1543	situ	1540:1543	Our research indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration.
29279118	6	36	theme	combined	1402:1409	arg1	group					1416:1420	the combined drug group	1398:1420	the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs)	1398:1471	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	4	37	theme	nanoparticle/hydrogel	884:904	arg1	compound					906:913	the nanoparticle/hydrogel compound	880:913	the nanoparticle/hydrogel compound	880:913	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	3	38	theme	cell	504:507	arg1	SDF-1α					528:533	SDF-1α	528:533	SDF-1α	528:533	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	3	38	theme	cell	504:507	arg1	factor-1α					517:525	stromal cell derived factor-1α	496:525	stromal cell derived factor-1α (SDF-1α)	496:534	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	5	39	theme	hydrogel	1147:1154	arg1	group					1156:1160	hydrogel group	1147:1160	hydrogel group	1147:1160	Comparing with blank group and hydrogel group, hydrogels incorporated with SDF-1α and/or CTH/antimiR-138 NPs significantly enhanced bone regeneration (p<0.05).
29279118	7	40	theme	NPs	1651:1653	arg1	sequence-release					1604:1619	temporal sequence-release	1595:1619	temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs	1595:1653	Our research indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration.
29279118	2	41	theme	spatiotemporal	317:330	arg1	sequence					332:339	a spatiotemporal sequence	315:339	a spatiotemporal sequence for adapting the demand of bone regeneration	315:384	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	1	42	theme	physiological	153:165	arg1	processes					167:175	complex physiological processes	145:175	complex physiological processes	145:175	Bone regeneration involves complex physiological processes, which is generally regulated and controlled by multiple bioactive molecules.
29279118	0	43	theme	stromal	30:36	arg1	factor-1α					51:59	stromal cell-derived factor-1α	30:59	stromal cell-derived factor-1α	30:59	In situ controlled release of stromal cell-derived factor-1α and antimiR-138 for on-demand cranial bone regeneration.
29279118	5	44	theme	bone	1248:1251	arg1	regeneration					1253:1264	bone regeneration	1248:1264	bone regeneration	1248:1264	Comparing with blank group and hydrogel group, hydrogels incorporated with SDF-1α and/or CTH/antimiR-138 NPs significantly enhanced bone regeneration (p<0.05).
29279118	1	45	theme	multiple	225:232	arg1	molecules					244:252	multiple bioactive molecules	225:252	multiple bioactive molecules	225:252	Bone regeneration involves complex physiological processes, which is generally regulated and controlled by multiple bioactive molecules.
29279118	3	46	theme	composite	446:454	arg1	system					456:461	nanoparticle/hydrogel composite system	424:461	nanoparticle/hydrogel composite system	424:461	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	7	47	theme	bone	1693:1696	arg1	regeneration					1698:1709	cranial bone regeneration	1685:1709	cranial bone regeneration	1685:1709	Our research indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration.
29279118	4	48	theme	calvarial	997:1005	arg1	specimens					1007:1015	calvarial specimens	997:1015	calvarial specimens	997:1015	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	3	49	theme	bone	721:724	arg1	regeneration					726:737	rat critical-size calvarial bone regeneration	693:737	rat critical-size calvarial bone regeneration	693:737	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	2	50	theme	In	255:256	arg1	release					274:280	In situ controlled release	255:280	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration	255:384	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	2	50	theme	In	255:256	arg1	strategy					399:406	a desired strategy	389:406	a desired strategy	389:406	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	4	51	from	compound	906:913	arg1	release					852:858	the sustained release	838:858	the sustained release of antimiR-138 from the nanoparticle/hydrogel compound	838:913	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	1	52	theme	bioactive	234:242	arg1	molecules					244:252	multiple bioactive molecules	225:252	multiple bioactive molecules	225:252	Bone regeneration involves complex physiological processes, which is generally regulated and controlled by multiple bioactive molecules.
29279118	0	53	theme	factor-1α	51:59	arg1	release					19:25	release	19:25	release of stromal cell-derived factor-1α	19:59	In situ controlled release of stromal cell-derived factor-1α and antimiR-138 for on-demand cranial bone regeneration.
29279118	7	54	theme	temporal	1595:1602	arg1	sequence-release					1604:1619	temporal sequence-release	1595:1619	temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs	1595:1653	Our research indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration.
29279118	3	55	theme	stromal	496:502	arg1	cell					504:507	stromal cell	496:507	stromal cell derived factor-1α (SDF-1α)	496:534	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	1	56	theme	Bone	118:121	arg1	regeneration					123:134	Bone regeneration	118:134	Bone regeneration	118:134	Bone regeneration involves complex physiological processes, which is generally regulated and controlled by multiple bioactive molecules.
29279118	0	57	theme	cell-derived	38:49	arg1	factor-1α					51:59	stromal cell-derived factor-1α	30:59	stromal cell-derived factor-1α	30:59	In situ controlled release of stromal cell-derived factor-1α and antimiR-138 for on-demand cranial bone regeneration.
29279118	4	58	theme	d.	971:972	arg1	weeks					976:980	21 d. 8 weeks	968:980	21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry	968:1113	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	5	59	theme	blank	1131:1135	arg1	group					1137:1141	blank group	1131:1141	blank group	1131:1141	Comparing with blank group and hydrogel group, hydrogels incorporated with SDF-1α and/or CTH/antimiR-138 NPs significantly enhanced bone regeneration (p<0.05).
29279118	3	60	theme	CS/GP	673:677	arg1	hydrogel					680:687	chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel	635:687	chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration	635:737	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	3	61	theme	chitosan/tripolyphosphate/hyaluronic	540:575	arg1	NPs					627:629	CTH/antimiR-138 NPs	611:629	CTH/antimiR-138 NPs	611:629	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	3	61	theme	chitosan/tripolyphosphate/hyaluronic	540:575	arg1	nanoparticles					596:608	chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles	540:608	chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs)	540:630	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	4	62	theme	mesenchymal	793:803	arg1	MSCs					817:820	MSCs	817:820	MSCs	817:820	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	4	62	theme	mesenchymal	793:803	arg1	cells					810:814	mesenchymal stem cells	793:814	mesenchymal stem cells (MSCs)	793:821	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	6	63	theme	type-1	1316:1321	arg1	proteins					1372:1379	collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins	1307:1379	collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins	1307:1379	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	3	64	theme	rat	693:695	arg1	regeneration					726:737	rat critical-size calvarial bone regeneration	693:737	rat critical-size calvarial bone regeneration	693:737	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	4	65	theme	stem	805:808	arg1	MSCs					817:820	MSCs	817:820	MSCs	817:820	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	4	65	theme	stem	805:808	arg1	cells					810:814	mesenchymal stem cells	793:814	mesenchymal stem cells (MSCs)	793:821	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	3	66	theme	critical-size	697:709	arg1	regeneration					726:737	rat critical-size calvarial bone regeneration	693:737	rat critical-size calvarial bone regeneration	693:737	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	2	67	theme	combined	285:292	arg1	factors					304:310	combined bioactive factors	285:310	combined bioactive factors	285:310	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	7	68	theme	in	1537:1538	arg1	formation					1545:1553	the in situ formation	1533:1553	the in situ formation of NPs/hydrogel composite	1533:1579	Our research indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration.
29279118	7	69	dep	indicated	1523:1531	arg1	provide					1587:1593	provide	1587:1593	indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration	1523:1709	Our research indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration.
29279118	7	70	theme	MSCs	1669:1672	arg1	homing					1674:1679	on-demand MSCs homing	1659:1679	on-demand MSCs homing	1659:1679	Our research indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration.
29279118	4	71	theme	histological	1068:1079	arg1	analysis					1081:1088	histological analysis	1068:1088	histological analysis	1068:1088	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	4	71	theme	histological	1068:1079	arg1	tomography					1049:1058	microcomputed tomography	1035:1058	microcomputed tomography (μ-CT)	1035:1065	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	3	72	theme	calvarial	711:719	arg1	regeneration					726:737	rat critical-size calvarial bone regeneration	693:737	rat critical-size calvarial bone regeneration	693:737	In this study, nanoparticle/hydrogel composite system was constructed by incorporating stromal cell derived factor-1α (SDF-1α) and chitosan/tripolyphosphate/hyaluronic acid/antimiRNA-138 nanoparticles (CTH/antimiR-138 NPs) in chitosan/β-sodium glycerol phosphate (CS/GP) hydrogel for rat critical-size calvarial bone regeneration.
29279118	1	73	theme	complex	145:151	arg1	processes					167:175	complex physiological processes	145:175	complex physiological processes	145:175	Bone regeneration involves complex physiological processes, which is generally regulated and controlled by multiple bioactive molecules.
29279118	2	74	dep	In	255:256	arg1	situ					258:261	situ	258:261	situ	258:261	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	4	75	theme	antimiR-138	863:873	arg1	release					852:858	the sustained release	838:858	the sustained release of antimiR-138 from the nanoparticle/hydrogel compound	838:913	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	7	76	theme	on-demand	1659:1667	arg1	homing					1674:1679	on-demand MSCs homing	1659:1679	on-demand MSCs homing	1659:1679	Our research indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration.
29279118	2	77	theme	desired	391:397	arg1	release					274:280	In situ controlled release	255:280	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration	255:384	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	2	77	theme	desired	391:397	arg1	strategy					399:406	a desired strategy	389:406	a desired strategy	389:406	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	4	78	dep	weeks	976:980	arg1	evaluated					1022:1030	evaluated	1022:1030	were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry	1017:1113	The fast release of SDF-1α promoted the migration of mesenchymal stem cells (MSCs) for 6 d, while the sustained release of antimiR-138 from the nanoparticle/hydrogel compound enhanced the osteogenic differentiation of MSCs over 21 d. 8 weeks after surgery, calvarial specimens were evaluated by microcomputed tomography (μ-CT), histological analysis and immunohistochemistry.
29279118	2	79	theme	controlled	263:272	arg1	release					274:280	In situ controlled release	255:280	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration	255:384	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	2	79	theme	controlled	263:272	arg1	strategy					399:406	a desired strategy	389:406	a desired strategy	389:406	In situ controlled release of combined bioactive factors in a spatiotemporal sequence for adapting the demand of bone regeneration is a desired strategy.
29279118	6	80	theme	collagen	1307:1314	arg1	COL-1					1324:1328	COL-1	1324:1328	COL-1	1324:1328	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	6	80	theme	collagen	1307:1314	arg1	type-1					1316:1321	collagen type-1	1307:1321	collagen type-1 (COL-1)	1307:1329	In addition, the expression of collagen type-1 (COL-1), osteopontin (OPN) and osteocalcin (OCN) proteins were enhanced in the combined drug group (incorporated both SDF-1α and CTH/antimiR-138 NPs) in comparison to the hydrogel group.
29279118	7	81	theme	NPs/hydrogel	1558:1569	arg1	composite					1571:1579	NPs/hydrogel composite	1558:1579	NPs/hydrogel composite	1558:1579	Our research indicated the in situ formation of NPs/hydrogel composite could provide temporal sequence-release of SDF-1α and CTH/antimiR-138 NPs for on-demand MSCs homing and cranial bone regeneration.
24520193	14	0	theme	DMAPA-Glyp	2311:2320	arg1	derivatives					2322:2332	the DMAPA-Glyp derivatives	2307:2332	especially the DMAPA-Glyp derivatives	2296:2332	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	14	0	theme	DMAPA-Glyp	2311:2320	arg1	derivatives					2283:2293	The hyperbranched cationic glycogen derivatives	2247:2293	The hyperbranched cationic glycogen derivatives	2247:2293	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	7	1	theme	nasopharyngeal	1345:1358	arg1	CNE2					1333:1336	the CNE2	1329:1336	the CNE2 (human nasopharyngeal carcinoma)	1329:1369	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	7	1	theme	nasopharyngeal	1345:1358	arg1	carcinoma					1360:1368	human nasopharyngeal carcinoma	1339:1368	human nasopharyngeal carcinoma	1339:1368	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	4	2	theme	Plasmid	639:645	arg1	pDNA					670:673	pDNA	670:673	pDNA	670:673	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	4	2	theme	Plasmid	639:645	arg1	acid					664:667	Plasmid deoxyribonucleic acid	639:667	Plasmid deoxyribonucleic acid (pDNA) condensation ability	639:695	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	14	3	theme	novel	2474:2478	arg1	which					2464:2468	which	2464:2468	which	2464:2468	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	14	3	theme	novel	2474:2478	arg1	vectors					2504:2510	novel potential nonviral gene vectors	2474:2510	novel potential nonviral gene vectors	2474:2510	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	3	4	theme	buffer	561:566	arg1	capacity					568:575	Their buffer capacity	555:575	Their buffer capacity	555:575	Their buffer capacity was assessed by acid-base titration in aqueous NaCl solution.
24520193	13	5	theme	control	2221:2227	arg1	group					2229:2233	the control group	2217:2233	the control group	2217:2233	Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group.
24520193	12	6	theme	DMAPA-Glyp/pDNA	1865:1879	arg1	complexes					1881:1889	the DMAPA-Glyp/pDNA complexes	1861:1889	the DMAPA-Glyp/pDNA complexes	1861:1889	The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI.
24520193	14	7	theme	potential	2480:2488	arg1	which					2464:2468	which	2464:2468	which	2464:2468	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	14	7	theme	potential	2480:2488	arg1	vectors					2504:2510	novel potential nonviral gene vectors	2474:2510	novel potential nonviral gene vectors	2474:2510	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	4	8	theme	acid	664:667	arg1	ability					689:695	Plasmid deoxyribonucleic acid (pDNA) condensation ability	639:695	Plasmid deoxyribonucleic acid (pDNA) condensation ability	639:695	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	9	9	theme	hyperbranched	1525:1537	arg1	derivatives					1557:1567	The hyperbranched cationic glycogen derivatives	1521:1567	The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues	1521:1607	RESULTS The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues were synthesized.
24520193	4	10	theme	I	726:726	arg1	degradation					728:738	DNase I degradation	720:738	DNase I degradation of the glycogen derivatives	720:766	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	14	11	theme	glycogen	2274:2281	arg1	derivatives					2322:2332	the DMAPA-Glyp derivatives	2307:2332	especially the DMAPA-Glyp derivatives	2296:2332	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	14	11	theme	glycogen	2274:2281	arg1	derivatives					2283:2293	The hyperbranched cationic glycogen derivatives	2247:2293	The hyperbranched cationic glycogen derivatives	2247:2293	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	2	12	theme	glycogen	299:306	arg1	derivatives					308:318	hyperbranched cationic glycogen derivatives	276:318	hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues	276:432	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	5	13	theme	complexes	935:943	arg1	images					921:926	the images	917:926	the images of the complexes	917:943	The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy.
24520193	12	14	theme	transfection	1834:1845	arg1	efficiency					1847:1856	The transfection efficiency	1830:1856	The transfection efficiency of the DMAPA-Glyp/pDNA complexes	1830:1889	The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI.
24520193	12	14	theme	transfection	1834:1845	arg1	higher					1895:1900	higher	1895:1900	higher	1895:1900	The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI.
24520193	14	15	theme	hyperbranched	2251:2263	arg1	derivatives					2322:2332	the DMAPA-Glyp derivatives	2307:2332	especially the DMAPA-Glyp derivatives	2296:2332	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	14	15	theme	hyperbranched	2251:2263	arg1	derivatives					2283:2293	The hyperbranched cationic glycogen derivatives	2247:2293	The hyperbranched cationic glycogen derivatives	2247:2293	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	7	16	theme	glycogen	1207:1214	arg1	derivatives					1216:1226	the cationic glycogen derivatives	1194:1226	the cationic glycogen derivatives	1194:1226	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	13	17	from	vessels	2075:2081	arg1	tissue					2092:2097	brain tissue	2086:2097	brain tissue of Sprague Dawley rats	2086:2120	Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group.
24520193	3	18	theme	aqueous	616:622	arg1	solution					629:636	aqueous NaCl solution	616:636	aqueous NaCl solution	616:636	Their buffer capacity was assessed by acid-base titration in aqueous NaCl solution.
24520193	1	19	theme	efficient	215:223	arg1	derivatives					197:207	hyperbranched cationic glycogen derivatives	165:207	hyperbranched cationic glycogen derivatives	165:207	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	1	19	theme	efficient	215:223	arg1	vector					248:253	an efficient nonviral gene-delivery vector	212:253	an efficient nonviral gene-delivery vector	212:253	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	6	20	theme	hemolysis	1045:1053	arg1	assay					1055:1059	hemolysis assay	1045:1059	hemolysis assay	1045:1059	Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively.
24520193	5	21	dep	potentials	826:835	arg1	The					817:819	The	817:819	The	817:819	The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy.
24520193	1	22	theme	gene-delivery	234:246	arg1	derivatives					197:207	hyperbranched cationic glycogen derivatives	165:207	hyperbranched cationic glycogen derivatives	165:207	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	1	22	theme	gene-delivery	234:246	arg1	vector					248:253	an efficient nonviral gene-delivery vector	212:253	an efficient nonviral gene-delivery vector	212:253	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	7	23	theme	transfection	1158:1169	arg1	efficiency					1171:1180	pDNA transfection efficiency	1153:1180	pDNA transfection efficiency mediated by the cationic glycogen derivatives	1153:1226	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	1	24	theme	study	128:132	arg1	purpose					112:118	The purpose	108:118	The purpose of this study	108:132	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	4	25	theme	gel	796:798	arg1	electrophoresis					800:814	agarose gel electrophoresis	788:814	agarose gel electrophoresis	788:814	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	13	26	theme	green	2175:2179	arg1	pDNA					2026:2029	pDNA	2026:2029	pDNA	2026:2029	Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group.
24520193	13	26	theme	green	2175:2179	arg1	protein					2194:2200	green fluorescence protein	2175:2200	green fluorescence protein	2175:2200	Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group.
24520193	2	27	theme	hyperbranched	276:288	arg1	derivatives					308:318	hyperbranched cationic glycogen derivatives	276:318	hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues	276:432	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	9	28	dep	RESULTS	1513:1519	arg1	synthesized					1614:1624	synthesized	1614:1624	were synthesized	1609:1624	RESULTS The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues were synthesized.
24520193	4	29	theme	glycogen	747:754	arg1	derivatives					756:766	the glycogen derivatives	743:766	the glycogen derivatives	743:766	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	7	30	theme	human	1339:1343	arg1	CNE2					1333:1336	the CNE2	1329:1336	the CNE2 (human nasopharyngeal carcinoma)	1329:1369	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	7	30	theme	human	1339:1343	arg1	carcinoma					1360:1368	human nasopharyngeal carcinoma	1339:1368	human nasopharyngeal carcinoma	1339:1368	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	5	31	theme	atomic	965:970	arg1	microscopy					978:987	atomic force microscopy	965:987	atomic force microscopy	965:987	The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy.
24520193	2	32	theme	resonance	531:539	arg1	spectroscopy					541:552	hydrogen-1 nuclear magnetic resonance spectroscopy	503:552	hydrogen-1 nuclear magnetic resonance spectroscopy	503:552	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	1	33	dep	BACKGROUND	97:106	arg1	was					134:136	was	134:136	was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector	134:253	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	14	34	theme	low	2407:2409	arg1	toxicity					2416:2423	low cyto toxicity	2407:2423	low cyto toxicity	2407:2423	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	1	35	theme	hyperbranched	165:177	arg1	derivatives					197:207	hyperbranched cationic glycogen derivatives	165:207	hyperbranched cationic glycogen derivatives	165:207	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	1	35	theme	hyperbranched	165:177	arg1	vector					248:253	an efficient nonviral gene-delivery vector	212:253	an efficient nonviral gene-delivery vector	212:253	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	11	36	from	complexes	1797:1805	arg1	size					1824:1827	size	1824:1827	size	1824:1827	They were able to bind and condense pDNA to form the complexes of 100-250 nm in size.
24520193	13	37	theme	rats	2117:2120	arg1	tissue					2092:2097	brain tissue	2086:2097	brain tissue of Sprague Dawley rats	2086:2120	Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group.
24520193	8	38	dep	In	1383:1384	arg1	vivo					1386:1389	vivo	1386:1389	vivo	1386:1389	In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency.
24520193	4	39	theme	agarose	788:794	arg1	electrophoresis					800:814	agarose gel electrophoresis	788:814	agarose gel electrophoresis	788:814	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	14	40	theme	blood	2382:2386	arg1	compatibility					2388:2400	good blood compatibility	2377:2400	good blood compatibility	2377:2400	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	5	41	theme	zeta	821:824	arg1	potentials					826:835	zeta potentials	821:835	zeta potentials	821:835	The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy.
24520193	7	42	theme	human	1301:1305	arg1	293T					1295:1298	the 293T	1291:1298	the 293T (human embryonic kidney)	1291:1323	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	7	42	theme	human	1301:1305	arg1	kidney					1317:1322	human embryonic kidney	1301:1322	human embryonic kidney	1301:1322	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	14	43	theme	gene-transfection	2347:2363	arg1	efficiency					2365:2374	high gene-transfection efficiency	2342:2374	high gene-transfection efficiency	2342:2374	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	0	44	theme	nonviral	13:20	arg1	vector					36:41	An efficient nonviral gene-delivery vector	0:41	An efficient nonviral gene-delivery vector	0:41	An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives.
24520193	2	45	theme	nuclear	514:520	arg1	resonance					531:539	hydrogen-1 nuclear magnetic resonance	503:539	hydrogen-1 nuclear magnetic resonance spectroscopy	503:552	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	11	46	theme	nm	1818:1819	arg1	complexes					1797:1805	the complexes	1793:1805	the complexes of 100-250 nm in size	1793:1827	They were able to bind and condense pDNA to form the complexes of 100-250 nm in size.
24520193	9	47	theme	cationic	1539:1546	arg1	derivatives					1557:1567	The hyperbranched cationic glycogen derivatives	1521:1567	The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues	1521:1607	RESULTS The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues were synthesized.
24520193	14	48	dep	CONCLUSION	2236:2245	arg1	showed					2335:2340	showed	2335:2340	showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors	2335:2510	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	9	49	theme	glycogen	1548:1555	arg1	derivatives					1557:1567	The hyperbranched cationic glycogen derivatives	1521:1567	The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues	1521:1607	RESULTS The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues were synthesized.
24520193	10	50	theme	blood	1649:1653	arg1	compatibility					1655:1667	better blood compatibility	1642:1667	better blood compatibility	1642:1667	They exhibited better blood compatibility and lower cytotoxicity when compared to branched polyethyleneimine (bPEI).
24520193	0	51	theme	cationic	66:73	arg1	derivatives					84:94	hyperbranched cationic glycogen derivatives	52:94	hyperbranched cationic glycogen derivatives	52:94	An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives.
24520193	5	52	theme	derivative/pDNA	872:886	arg1	complexes					888:896	the glycogen derivative/pDNA complexes	859:896	the glycogen derivative/pDNA complexes	859:896	The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy.
24520193	13	53	theme	Dawley	2110:2115	arg1	rats					2117:2120	Sprague Dawley rats	2102:2120	Sprague Dawley rats	2102:2120	Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group.
24520193	14	54	theme	gene	2499:2502	arg1	which					2464:2468	which	2464:2468	which	2464:2468	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	14	54	theme	gene	2499:2502	arg1	vectors					2504:2510	novel potential nonviral gene vectors	2474:2510	novel potential nonviral gene vectors	2474:2510	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	1	55	theme	glycogen	188:195	arg1	derivatives					197:207	hyperbranched cationic glycogen derivatives	165:207	hyperbranched cationic glycogen derivatives	165:207	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	1	55	theme	glycogen	188:195	arg1	vector					248:253	an efficient nonviral gene-delivery vector	212:253	an efficient nonviral gene-delivery vector	212:253	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	0	56	theme	hyperbranched	52:64	arg1	derivatives					84:94	hyperbranched cationic glycogen derivatives	52:94	hyperbranched cationic glycogen derivatives	52:94	An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives.
24520193	7	57	theme	cell	1371:1374	arg1	lines					1376:1380	cell lines	1371:1380	cell lines	1371:1380	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	12	58	dep	293T	1957:1960	arg1	the					1953:1955	the	1953:1955	the	1953:1955	The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI.
24520193	7	59	theme	flow	1245:1248	arg1	cytometry					1250:1258	flow cytometry	1245:1258	flow cytometry	1245:1258	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	13	60	theme	brain	2086:2090	arg1	tissue					2092:2097	brain tissue	2086:2097	brain tissue of Sprague Dawley rats	2086:2120	Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group.
24520193	2	61	theme	1-	385:386	arg1	AEPZ-Glyp					414:422	AEPZ-Glyp	414:422	AEPZ-Glyp	414:422	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	2	61	theme	1-	385:386	arg1	piperazine					402:411	1-(2-aminoethyl) piperazine	385:411	1-(2-aminoethyl) piperazine (AEPZ-Glyp)	385:423	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	6	62	theme	Blood	990:994	arg1	compatibility					996:1008	Blood compatibility	990:1008	Blood compatibility	990:1008	Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively.
24520193	12	63	theme	complexes	1881:1889	arg1	efficiency					1847:1856	The transfection efficiency	1830:1856	The transfection efficiency of the DMAPA-Glyp/pDNA complexes	1830:1889	The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI.
24520193	12	63	theme	complexes	1881:1889	arg1	higher					1895:1900	higher	1895:1900	higher	1895:1900	The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI.
24520193	10	64	theme	branched	1709:1716	arg1	bPEI					1737:1740	bPEI	1737:1740	bPEI	1737:1740	They exhibited better blood compatibility and lower cytotoxicity when compared to branched polyethyleneimine (bPEI).
24520193	10	64	theme	branched	1709:1716	arg1	polyethyleneimine					1718:1734	branched polyethyleneimine	1709:1734	branched polyethyleneimine (bPEI)	1709:1741	They exhibited better blood compatibility and lower cytotoxicity when compared to branched polyethyleneimine (bPEI).
24520193	3	65	theme	acid-base	593:601	arg1	titration					603:611	acid-base titration	593:611	acid-base titration	593:611	Their buffer capacity was assessed by acid-base titration in aqueous NaCl solution.
24520193	4	66	theme	deoxyribonucleic	647:662	arg1	pDNA					670:673	pDNA	670:673	pDNA	670:673	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	4	66	theme	deoxyribonucleic	647:662	arg1	acid					664:667	Plasmid deoxyribonucleic acid	639:667	Plasmid deoxyribonucleic acid (pDNA) condensation ability	639:695	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	2	67	theme	derivatives	308:318	arg1	series					266:271	A series	264:271	A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues	264:432	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	1	68	theme	cationic	179:186	arg1	derivatives					197:207	hyperbranched cationic glycogen derivatives	165:207	hyperbranched cationic glycogen derivatives	165:207	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	1	68	theme	cationic	179:186	arg1	vector					248:253	an efficient nonviral gene-delivery vector	212:253	an efficient nonviral gene-delivery vector	212:253	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	7	69	theme	fluorescence	1264:1275	arg1	microscopy					1277:1286	fluorescence microscopy	1264:1286	fluorescence microscopy	1264:1286	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	4	70	theme	DNase	720:724	arg1	degradation					728:738	DNase I degradation	720:738	DNase I degradation of the glycogen derivatives	720:766	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	14	71	theme	cationic	2265:2272	arg1	derivatives					2322:2332	the DMAPA-Glyp derivatives	2307:2332	especially the DMAPA-Glyp derivatives	2296:2332	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	14	71	theme	cationic	2265:2272	arg1	derivatives					2283:2293	The hyperbranched cationic glycogen derivatives	2247:2293	The hyperbranched cationic glycogen derivatives	2247:2293	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	7	72	theme	cationic	1198:1205	arg1	derivatives					1216:1226	the cationic glycogen derivatives	1194:1226	the cationic glycogen derivatives	1194:1226	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	3	73	theme	NaCl	624:627	arg1	solution					629:636	aqueous NaCl solution	616:636	aqueous NaCl solution	616:636	Their buffer capacity was assessed by acid-base titration in aqueous NaCl solution.
24520193	6	74	theme	MTT	1065:1067	arg1	assay					1132:1136	MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay	1065:1136	MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay	1065:1136	Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively.
24520193	1	75	theme	nonviral	225:232	arg1	derivatives					197:207	hyperbranched cationic glycogen derivatives	165:207	hyperbranched cationic glycogen derivatives	165:207	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	1	75	theme	nonviral	225:232	arg1	vector					248:253	an efficient nonviral gene-delivery vector	212:253	an efficient nonviral gene-delivery vector	212:253	BACKGROUND The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector.
24520193	2	76	dep	METHODS	256:262	arg1	characterized					455:467	characterized	455:467	characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy	455:552	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	2	76	dep	METHODS	256:262	arg1	synthesized					439:449	synthesized	439:449	synthesized	439:449	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	7	77	theme	pDNA	1153:1156	arg1	efficiency					1171:1180	pDNA transfection efficiency	1153:1180	pDNA transfection efficiency mediated by the cationic glycogen derivatives	1153:1226	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	2	78	theme	cationic	290:297	arg1	derivatives					308:318	hyperbranched cationic glycogen derivatives	276:318	hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues	276:432	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	8	79	theme	pDNA	1403:1406	arg1	delivery					1391:1398	In vivo delivery	1383:1398	In vivo delivery of pDNA in model animals (Sprague Dawley rats)	1383:1445	In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency.
24520193	0	80	theme	glycogen	75:82	arg1	derivatives					84:94	hyperbranched cationic glycogen derivatives	52:94	hyperbranched cationic glycogen derivatives	52:94	An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives.
24520193	13	81	theme	fluorescence	2181:2192	arg1	pDNA					2026:2029	pDNA	2026:2029	pDNA	2026:2029	Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group.
24520193	13	81	theme	fluorescence	2181:2192	arg1	protein					2194:2200	green fluorescence protein	2175:2200	green fluorescence protein	2175:2200	Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group.
24520193	8	82	theme	model	1411:1415	arg1	animals					1417:1423	model animals	1411:1423	model animals (Sprague Dawley rats)	1411:1445	In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency.
24520193	8	82	theme	model	1411:1415	arg1	rats					1441:1444	Sprague Dawley rats	1426:1444	Sprague Dawley rats	1426:1444	In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency.
24520193	14	83	theme	cyto	2411:2414	arg1	toxicity					2416:2423	low cyto toxicity	2407:2423	low cyto toxicity	2407:2423	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	5	84	theme	particle	841:848	arg1	sizes					850:854	particle sizes	841:854	particle sizes	841:854	The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy.
24520193	5	85	theme	force	972:976	arg1	microscopy					978:987	atomic force microscopy	965:987	atomic force microscopy	965:987	The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy.
24520193	6	86	theme	-2,5-diphenyltetrazolium	1098:1121	arg1	bromide					1123:1129	3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide	1070:1129	3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide	1070:1129	Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively.
24520193	6	86	theme	-2,5-diphenyltetrazolium	1098:1121	arg1	MTT					1065:1067	MTT	1065:1067	MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay	1065:1136	Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively.
24520193	2	87	theme	magnetic	522:529	arg1	resonance					531:539	hydrogen-1 nuclear magnetic resonance	503:539	hydrogen-1 nuclear magnetic resonance spectroscopy	503:552	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	7	88	dep	293T	1295:1298	arg1	lines					1376:1380	cell lines	1371:1380	cell lines	1371:1380	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	4	89	theme	derivatives	756:766	arg1	degradation					728:738	DNase I degradation	720:738	DNase I degradation of the glycogen derivatives	720:766	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	2	90	theme	3-	336:337	arg1	DMAPA-Glyp					369:378	DMAPA-Glyp	369:378	DMAPA-Glyp	369:378	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	2	90	theme	3-	336:337	arg1	-1-propylamine					353:366	3-(dimethylamino)-1-propylamine	336:366	3-(dimethylamino)-1-propylamine (DMAPA-Glyp)	336:379	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	6	91	theme	3-[4,5-dimethylthiazol-2-yl	1070:1096	arg1	bromide					1123:1129	3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide	1070:1129	3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide	1070:1129	Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively.
24520193	6	91	theme	3-[4,5-dimethylthiazol-2-yl	1070:1096	arg1	MTT					1065:1067	MTT	1065:1067	MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay	1065:1136	Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively.
24520193	8	92	theme	Dawley	1434:1439	arg1	animals					1417:1423	model animals	1411:1423	model animals (Sprague Dawley rats)	1411:1445	In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency.
24520193	8	92	theme	Dawley	1434:1439	arg1	rats					1441:1444	Sprague Dawley rats	1426:1444	Sprague Dawley rats	1426:1444	In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency.
24520193	7	93	theme	embryonic	1307:1315	arg1	293T					1295:1298	the 293T	1291:1298	the 293T (human embryonic kidney)	1291:1323	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	7	93	theme	embryonic	1307:1315	arg1	kidney					1317:1322	human embryonic kidney	1301:1322	human embryonic kidney	1301:1322	pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines.
24520193	8	94	from	delivery	1391:1398	arg1	animals					1417:1423	model animals	1411:1423	model animals (Sprague Dawley rats)	1411:1445	In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency.
24520193	8	94	from	delivery	1391:1398	arg1	rats					1441:1444	Sprague Dawley rats	1426:1444	Sprague Dawley rats	1426:1444	In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency.
24520193	14	95	theme	good	2377:2380	arg1	compatibility					2388:2400	good blood compatibility	2377:2400	good blood compatibility	2377:2400	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	0	96	theme	efficient	3:11	arg1	vector					36:41	An efficient nonviral gene-delivery vector	0:41	An efficient nonviral gene-delivery vector	0:41	An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives.
24520193	13	97	theme	DMAPA-Glyp	2129:2138	arg1	derivatives					2140:2150	the DMAPA-Glyp derivatives	2125:2150	the DMAPA-Glyp derivatives	2125:2150	Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group.
24520193	2	98	dep	-1-propylamine	353:366	arg1	residues					425:432	residues	425:432	residues	425:432	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	0	99	theme	gene-delivery	22:34	arg1	vector					36:41	An efficient nonviral gene-delivery vector	0:41	An efficient nonviral gene-delivery vector	0:41	An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives.
24520193	14	100	theme	high	2342:2345	arg1	efficiency					2365:2374	high gene-transfection efficiency	2342:2374	high gene-transfection efficiency	2342:2374	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	2	101	theme	hydrogen-1	503:512	arg1	resonance					531:539	hydrogen-1 nuclear magnetic resonance	503:539	hydrogen-1 nuclear magnetic resonance spectroscopy	503:552	METHODS A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy.
24520193	5	102	theme	glycogen	863:870	arg1	complexes					888:896	the glycogen derivative/pDNA complexes	859:896	the glycogen derivative/pDNA complexes	859:896	The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy.
24520193	12	103	theme	AEPZ-Glyp/pDNA	1920:1933	arg1	complexes					1935:1943	the AEPZ-Glyp/pDNA complexes	1916:1943	the AEPZ-Glyp/pDNA complexes	1916:1943	The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI.
24520193	10	104	theme	better	1642:1647	arg1	compatibility					1655:1667	better blood compatibility	1642:1667	better blood compatibility	1642:1667	They exhibited better blood compatibility and lower cytotoxicity when compared to branched polyethyleneimine (bPEI).
24520193	8	105	theme	transfection	1488:1499	arg1	efficiency					1501:1510	transfection efficiency	1488:1510	transfection efficiency	1488:1510	In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency.
24520193	12	106	theme	CNE2	1966:1969	arg1	cells					1971:1975	CNE2 cells	1966:1975	CNE2 cells	1966:1975	The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI.
24520193	13	107	theme	blood	2069:2073	arg1	vessels					2075:2081	the blood vessels	2065:2081	the blood vessels in brain tissue of Sprague Dawley rats	2065:2120	Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group.
24520193	8	108	theme	In	1383:1384	arg1	delivery					1391:1398	In vivo delivery	1383:1398	In vivo delivery of pDNA in model animals (Sprague Dawley rats)	1383:1445	In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency.
24520193	4	109	theme	condensation	676:687	arg1	ability					689:695	Plasmid deoxyribonucleic acid (pDNA) condensation ability	639:695	Plasmid deoxyribonucleic acid (pDNA) condensation ability	639:695	Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis.
24520193	10	110	theme	lower	1673:1677	arg1	cytotoxicity					1679:1690	lower cytotoxicity	1673:1690	lower cytotoxicity	1673:1690	They exhibited better blood compatibility and lower cytotoxicity when compared to branched polyethyleneimine (bPEI).
24520193	9	111	dep	DMAPA	1585:1589	arg1	residues					1600:1607	residues	1600:1607	residues	1600:1607	RESULTS The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues were synthesized.
24520193	14	112	theme	nonviral	2490:2497	arg1	which					2464:2468	which	2464:2468	which	2464:2468	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	14	112	theme	nonviral	2490:2497	arg1	vectors					2504:2510	novel potential nonviral gene vectors	2474:2510	novel potential nonviral gene vectors	2474:2510	CONCLUSION The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.
24520193	5	113	theme	complexes	888:896	arg1	potentials					826:835	zeta potentials	821:835	zeta potentials	821:835	The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy.
24520193	5	113	theme	complexes	888:896	arg1	sizes					850:854	particle sizes	841:854	particle sizes	841:854	The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy.
27795327	6	0	theme	membrane	828:835	arg1	integrity					837:845	membrane integrity	828:845	membrane integrity	828:845	Here, we show that these two proteins are components of novel transport pathways that contribute to membrane integrity.
27795327	13	1	theme	stress	1641:1646	arg1	resistance					1648:1657	stress resistance	1641:1657	stress resistance	1641:1657	IMPORTANCE Membranes of bacteria are crucial for stress resistance.
27795327	14	2	dep	E.	1683:1684	arg1	coli					1686:1689	coli	1686:1689	coli	1686:1689	The composition of the E. coli outer membrane is asymmetric, with asymmetry maintained by the Mla ABC transport pathway.
27795327	10	3	from	bacteria	1077:1084	arg1	located					1103:1109	located	1103:1109	located	1103:1109	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	17	4	theme	mutant	2187:2192	arg1	strains					2194:2200	mutant strains	2187:2200	mutant strains with defective LPS molecules	2187:2229	We found that yebS'-'lacZ fusion was activated in mutant strains with defective LPS molecules.
27795327	2	5	theme	asymmetric	256:265	arg1	bilayer					267:273	an asymmetric bilayer	253:273	an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively	253:386	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	2	5	theme	asymmetric	256:265	arg1	membrane					215:222	The outer membrane	205:222	The outer membrane of Gram-negative bacteria	205:248	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	6	6	theme	pathways	800:807	arg1	components					770:779	components	770:779	components of novel transport pathways that contribute to membrane integrity	770:845	Here, we show that these two proteins are components of novel transport pathways that contribute to membrane integrity.
27795327	6	6	theme	pathways	800:807	arg1	proteins					757:764	these two proteins	747:764	these two proteins	747:764	Here, we show that these two proteins are components of novel transport pathways that contribute to membrane integrity.
27795327	13	7	theme	bacteria	1616:1623	arg1	Membranes					1603:1611	IMPORTANCE Membranes	1592:1611	IMPORTANCE Membranes of bacteria	1592:1623	IMPORTANCE Membranes of bacteria are crucial for stress resistance.
27795327	11	8	from	defect	1431:1436	arg1	molecules					1445:1453	LPS molecules	1441:1453	LPS molecules	1441:1453	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	6	9	theme	novel	784:788	arg1	pathways					800:807	novel transport pathways	784:807	novel transport pathways that contribute to membrane integrity	784:845	Here, we show that these two proteins are components of novel transport pathways that contribute to membrane integrity.
27795327	8	10	theme	yebST	900:904	arg1	operon					906:911	The yebST operon	896:911	The yebST operon	896:911	The yebST operon contains pqiAB homologues.
27795327	5	11	theme	cell	612:615	arg1	MCE					624:626	MCE	624:626	MCE	624:626	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	5	11	theme	cell	612:615	arg1	entry					617:621	mammalian cell entry	602:621	a mammalian cell entry (MCE) domain protein	600:642	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	11	12	from	background	1237:1246	arg1	deletion					1189:1196	simultaneous deletion	1176:1196	simultaneous deletion of pqiABC and yebST operons in an Δmla background	1176:1246	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	1	13	theme	first	182:186	arg1	line					188:191	the first line	178:191	the first line of defense	178:202	The membranes of single-cell organisms are crucial as the first line of defense.
27795327	11	14	theme	yebST	1212:1216	arg1	operons					1218:1224	pqiABC and yebST operons	1201:1224	pqiABC and yebST operons in an Δmla background	1201:1246	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	14	15	theme	outer	1691:1695	arg1	membrane					1697:1704	the E. coli outer membrane	1679:1704	the E. coli outer membrane	1679:1704	The composition of the E. coli outer membrane is asymmetric, with asymmetry maintained by the Mla ABC transport pathway.
27795327	16	16	theme	membrane	2029:2036	arg1	stress					2038:2043	membrane stress	2029:2043	membrane stress	2029:2043	Deletion of these operons rendered cells more sensitive to membrane stress, and additional copies of pqiABC suppressed the SDS-EDTA sensitivity of mla mutant strains.
27795327	17	17	theme	defective	2207:2215	arg1	molecules					2221:2229	defective LPS molecules	2207:2229	defective LPS molecules	2207:2229	We found that yebS'-'lacZ fusion was activated in mutant strains with defective LPS molecules.
27795327	11	18	theme	pqiABC	1201:1206	arg1	operons					1218:1224	pqiABC and yebST operons	1201:1224	pqiABC and yebST operons in an Δmla background	1201:1246	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	11	19	from	operons	1218:1224	arg1	background					1237:1246	an Δmla background	1229:1246	an Δmla background	1229:1246	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	14	20	theme	E.	1683:1684	arg1	membrane					1697:1704	the E. coli outer membrane	1679:1704	the E. coli outer membrane	1679:1704	The composition of the E. coli outer membrane is asymmetric, with asymmetry maintained by the Mla ABC transport pathway.
27795327	12	21	from	pathways	1508:1515	arg1	conclusion					1459:1468	conclusion	1459:1468	conclusion	1459:1468	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	8	22	contain	contains	913:920	arg1	operon					906:911	The yebST operon	896:911	The yebST operon	896:911	The yebST operon contains pqiAB homologues.
27795327	8	22	contain	contains	913:920	arg2	homologues					928:937	pqiAB homologues	922:937	pqiAB homologues	922:937	The yebST operon contains pqiAB homologues.
27795327	2	23	theme	Gram-negative	227:239	arg1	bacteria					241:248	Gram-negative bacteria	227:248	Gram-negative bacteria	227:248	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	12	24	theme	Mla	1532:1534	arg1	pathway					1546:1552	the Mla transport pathway	1528:1552	the Mla transport pathway	1528:1552	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	5	25	dep	has	657:659	arg1	YebT					721:724	YebT	721:724	YebT	721:724	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	5	25	dep	has	657:659	arg1	PqiB					712:715	PqiB	712:715	PqiB	712:715	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	11	26	theme	mla	1323:1325	arg1	mutants					1327:1333	mla mutants	1323:1333	mla mutants	1323:1333	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	5	27	theme	unknown	694:700	arg1	function					702:709	unknown function	694:709	unknown function	694:709	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	12	28	theme	novel	1492:1496	arg1	PqiABC					1471:1476	PqiABC	1471:1476	PqiABC	1471:1476	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	12	28	theme	novel	1492:1496	arg1	pathways					1508:1515	novel transport pathways	1492:1515	novel transport pathways related to the Mla transport pathway and important for membrane integrity	1492:1589	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	12	28	theme	novel	1492:1496	arg1	YebST					1482:1486	YebST	1482:1486	YebST	1482:1486	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	4	29	theme	Mla	466:468	arg1	pathway					480:486	the Mla transport pathway	462:486	the Mla transport pathway	462:486	In Escherichia coli, the Mla transport pathway maintains this asymmetry by removing phospholipids from the outer leaflet.
27795327	16	30	theme	strains	2128:2134	arg1	sensitivity					2102:2112	the SDS-EDTA sensitivity	2089:2112	the SDS-EDTA sensitivity of mla mutant strains	2089:2134	Deletion of these operons rendered cells more sensitive to membrane stress, and additional copies of pqiABC suppressed the SDS-EDTA sensitivity of mla mutant strains.
27795327	1	31	theme	single-cell	141:151	arg1	organisms					153:161	single-cell organisms	141:161	single-cell organisms	141:161	The membranes of single-cell organisms are crucial as the first line of defense.
27795327	2	32	located	localized	333:341	arg1	leaflet					366:372	the outer and inner leaflet	346:372	the outer and inner leaflet	346:372	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	2	32	located	localized	333:341	arg2	LPSs					305:308	LPSs	305:308	LPSs	305:308	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	2	32	located	localized	333:341	arg2	phospholipids					315:327	phospholipids	315:327	phospholipids	315:327	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	2	32	located	localized	333:341	arg2	lipopolysaccharides					284:302	lipopolysaccharides	284:302	lipopolysaccharides (LPSs)	284:309	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	2	32	located	localized	333:341	arg1	bilayer					267:273	an asymmetric bilayer	253:273	an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively	253:386	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	2	32	located	localized	333:341	arg1	membrane					215:222	The outer membrane	205:222	The outer membrane of Gram-negative bacteria	205:248	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	9	33	theme	third	957:961	arg1	member					963:968	a third member	955:968	a third member of the pqi operon	955:986	Here, we found a third member of the pqi operon, ymbA (pqiC).
27795327	9	33	theme	third	957:961	arg1	ymbA					989:992	ymbA	989:992	ymbA (pqiC)	989:999	Here, we found a third member of the pqi operon, ymbA (pqiC).
27795327	0	34	theme	Transport	67:75	arg1	yebST					11:15	yebST	11:15	yebST	11:15	pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity.
27795327	0	34	theme	Transport	67:75	arg1	Pathways					77:84	Transport Pathways	67:84	Transport Pathways	67:84	pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity.
27795327	0	34	theme	Transport	67:75	arg1	pqiABC					0:5	pqiABC	0:5	pqiABC	0:5	pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity.
27795327	7	35	theme	pqiAB	852:856	arg1	operon					858:863	The pqiAB operon	848:863	The pqiAB operon	848:863	The pqiAB operon is regulated by SoxS and RpoS.
27795327	10	36	theme	pqiBC	1087:1091	arg1	genes					1093:1097	pqiBC genes	1087:1097	pqiBC genes	1087:1097	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	9	37	theme	pqi	977:979	arg1	operon					981:986	the pqi operon	973:986	the pqi operon	973:986	Here, we found a third member of the pqi operon, ymbA (pqiC).
27795327	17	38	theme	LPS	2217:2219	arg1	molecules					2221:2229	defective LPS molecules	2207:2229	defective LPS molecules	2207:2229	We found that yebS'-'lacZ fusion was activated in mutant strains with defective LPS molecules.
27795327	5	39	contain	has	657:659	arg1	coli					652:655	E. coli	649:655	E. coli	649:655	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	5	39	contain	has	657:659	arg2	proteins					682:689	two other MCE domain proteins	661:689	two other MCE domain proteins of unknown function	661:709	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	11	40	dep	copies	1361:1366	arg1	found					1386:1390	found	1386:1390	found that the yebST operon was induced by a defect in LPS molecules	1386:1453	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	16	41	theme	pqiABC	2071:2076	arg1	copies					2061:2066	additional copies	2050:2066	additional copies of pqiABC	2050:2076	Deletion of these operons rendered cells more sensitive to membrane stress, and additional copies of pqiABC suppressed the SDS-EDTA sensitivity of mla mutant strains.
27795327	15	42	theme	pqiABC	1818:1823	arg1	operon					1825:1830	the stress-inducible pqiABC operon	1797:1830	the stress-inducible pqiABC operon	1797:1830	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	42	theme	pqiABC	1818:1823	arg1	operon					1853:1858	homologous yebST operon	1836:1858	homologous yebST operon	1836:1858	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	42	theme	pqiABC	1818:1823	arg1	proteins					1923:1930	transport pathway proteins	1905:1930	transport pathway proteins related to the Mla transport pathway	1905:1967	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	42	theme	pqiABC	1818:1823	arg1	function					1888:1895	previously unknown function	1869:1895	previously unknown function	1869:1895	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	10	43	theme	other	1071:1075	arg1	bacteria					1077:1084	other bacteria	1071:1084	other bacteria	1071:1084	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	3	44	theme	membrane	421:428	arg1	integrity					430:438	membrane integrity	421:438	membrane integrity	421:438	This asymmetry is important for membrane integrity.
27795327	0	45	theme	Putative	18:25	arg1	pqiABC					0:5	pqiABC	0:5	pqiABC	0:5	pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity.
27795327	0	45	theme	Putative	18:25	arg1	Operons					31:37	Putative mce Operons	18:37	Putative mce Operons of Escherichia coli	18:57	pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity.
27795327	14	46	with	asymmetric	1709:1718	arg1	asymmetry					1726:1734	asymmetry	1726:1734	asymmetry maintained by the Mla ABC transport pathway	1726:1778	The composition of the E. coli outer membrane is asymmetric, with asymmetry maintained by the Mla ABC transport pathway.
27795327	14	47	theme	transport	1762:1770	arg1	pathway					1772:1778	the Mla ABC transport pathway	1750:1778	the Mla ABC transport pathway	1750:1778	The composition of the E. coli outer membrane is asymmetric, with asymmetry maintained by the Mla ABC transport pathway.
27795327	10	48	theme	outer	1048:1052	arg1	membrane					1054:1061	the outer membrane	1044:1061	the outer membrane	1044:1061	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	14	49	theme	Mla	1754:1756	arg1	pathway					1772:1778	the Mla ABC transport pathway	1750:1778	the Mla ABC transport pathway	1750:1778	The composition of the E. coli outer membrane is asymmetric, with asymmetry maintained by the Mla ABC transport pathway.
27795327	17	50	theme	yebS'-'lacZ	2151:2161	arg1	fusion					2163:2168	yebS'-'lacZ fusion	2151:2168	yebS'-'lacZ fusion	2151:2168	We found that yebS'-'lacZ fusion was activated in mutant strains with defective LPS molecules.
27795327	11	51	theme	LPS	1441:1443	arg1	molecules					1445:1453	LPS molecules	1441:1453	LPS molecules	1441:1453	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	11	52	theme	operons	1218:1224	arg1	deletion					1189:1196	simultaneous deletion	1176:1196	simultaneous deletion of pqiABC and yebST operons in an Δmla background	1176:1246	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	5	53	theme	entry	617:621	arg1	protein					636:642	a mammalian cell entry (MCE) domain protein	600:642	a mammalian cell entry (MCE) domain protein	600:642	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	5	53	theme	entry	617:621	arg1	component					572:580	The MlaD component	563:580	The MlaD component of this system	563:595	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	12	54	theme	membrane	1572:1579	arg1	integrity					1581:1589	membrane integrity	1572:1589	membrane integrity	1572:1589	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	13	55	theme	IMPORTANCE	1592:1601	arg1	Membranes					1603:1611	IMPORTANCE Membranes	1592:1611	IMPORTANCE Membranes of bacteria	1592:1623	IMPORTANCE Membranes of bacteria are crucial for stress resistance.
27795327	12	56	theme	transport	1536:1544	arg1	pathway					1546:1552	the Mla transport pathway	1528:1552	the Mla transport pathway	1528:1552	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	11	57	theme	pqiABC	1371:1376	arg1	copies					1361:1366	additional copies	1350:1366	additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules	1350:1453	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	1	58	theme	organisms	153:161	arg1	crucial					167:173	crucial	167:173	crucial	167:173	The membranes of single-cell organisms are crucial as the first line of defense.
27795327	1	58	theme	organisms	153:161	arg1	membranes					128:136	The membranes	124:136	The membranes of single-cell organisms	124:161	The membranes of single-cell organisms are crucial as the first line of defense.
27795327	6	59	theme	transport	790:798	arg1	pathways					800:807	novel transport pathways	784:807	novel transport pathways that contribute to membrane integrity	784:845	Here, we show that these two proteins are components of novel transport pathways that contribute to membrane integrity.
27795327	11	60	theme	simultaneous	1176:1187	arg1	deletion					1189:1196	simultaneous deletion	1176:1196	simultaneous deletion of pqiABC and yebST operons in an Δmla background	1176:1246	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	5	61	theme	mammalian	602:610	arg1	MCE					624:626	MCE	624:626	MCE	624:626	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	5	61	theme	mammalian	602:610	arg1	entry					617:621	mammalian cell entry	602:621	a mammalian cell entry (MCE) domain protein	600:642	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	11	62	theme	yebST	1401:1405	arg1	operon					1407:1412	the yebST operon	1397:1412	the yebST operon	1397:1412	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	8	63	theme	pqiAB	922:926	arg1	homologues					928:937	pqiAB homologues	922:937	pqiAB homologues	922:937	The yebST operon contains pqiAB homologues.
27795327	12	64	theme	important	1558:1566	arg1	PqiABC					1471:1476	PqiABC	1471:1476	PqiABC	1471:1476	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	12	64	theme	important	1558:1566	arg1	pathways					1508:1515	novel transport pathways	1492:1515	novel transport pathways related to the Mla transport pathway and important for membrane integrity	1492:1589	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	12	64	theme	important	1558:1566	arg1	YebST					1482:1486	YebST	1482:1486	YebST	1482:1486	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	0	65	theme	Membrane	104:111	arg1	Integrity					113:121	Membrane Integrity	104:121	Membrane Integrity	104:121	pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity.
27795327	15	66	theme	related	1932:1938	arg1	operon					1825:1830	the stress-inducible pqiABC operon	1797:1830	the stress-inducible pqiABC operon	1797:1830	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	66	theme	related	1932:1938	arg1	operon					1853:1858	homologous yebST operon	1836:1858	homologous yebST operon	1836:1858	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	66	theme	related	1932:1938	arg1	proteins					1923:1930	transport pathway proteins	1905:1930	transport pathway proteins related to the Mla transport pathway	1905:1967	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	66	theme	related	1932:1938	arg1	function					1888:1895	previously unknown function	1869:1895	previously unknown function	1869:1895	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	10	67	located	located	1103:1109	arg1	operons					1114:1120	operons	1114:1120	operons together with transporter proteins	1114:1155	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	10	67	located	located	1103:1109	arg2	genes					1093:1097	pqiBC genes	1087:1097	pqiBC genes	1087:1097	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	10	67	located	located	1103:1109	arg1	bacteria					1077:1084	other bacteria	1071:1084	other bacteria	1071:1084	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	10	68	from	located	1103:1109	arg1	operons					1114:1120	operons	1114:1120	operons together with transporter proteins	1114:1155	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	10	68	from	located	1103:1109	arg1	bacteria					1077:1084	other bacteria	1071:1084	other bacteria	1071:1084	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	2	69	theme	outer	209:213	arg1	bilayer					267:273	an asymmetric bilayer	253:273	an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively	253:386	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	2	69	theme	outer	209:213	arg1	membrane					215:222	The outer membrane	205:222	The outer membrane of Gram-negative bacteria	205:248	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	14	70	theme	membrane	1697:1704	arg1	asymmetric					1709:1718	asymmetric	1709:1718	asymmetric	1709:1718	The composition of the E. coli outer membrane is asymmetric, with asymmetry maintained by the Mla ABC transport pathway.
27795327	14	70	theme	membrane	1697:1704	arg1	composition					1664:1674	The composition	1660:1674	The composition of the E. coli outer membrane	1660:1704	The composition of the E. coli outer membrane is asymmetric, with asymmetry maintained by the Mla ABC transport pathway.
27795327	10	71	from	operons	1114:1120	arg1	located					1103:1109	located	1103:1109	located	1103:1109	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	16	72	theme	operons	1988:1994	arg1	Deletion					1970:1977	Deletion	1970:1977	Deletion of these operons	1970:1994	Deletion of these operons rendered cells more sensitive to membrane stress, and additional copies of pqiABC suppressed the SDS-EDTA sensitivity of mla mutant strains.
27795327	5	73	theme	domain	629:634	arg1	protein					636:642	a mammalian cell entry (MCE) domain protein	600:642	a mammalian cell entry (MCE) domain protein	600:642	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	5	73	theme	domain	629:634	arg1	component					572:580	The MlaD component	563:580	The MlaD component of this system	563:595	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	11	74	theme	mutants	1327:1333	arg1	sensitivity					1308:1318	the SDS-EDTA sensitivity	1295:1318	the SDS-EDTA sensitivity of mla mutants	1295:1333	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	15	75	theme	transport	1905:1913	arg1	operon					1825:1830	the stress-inducible pqiABC operon	1797:1830	the stress-inducible pqiABC operon	1797:1830	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	75	theme	transport	1905:1913	arg1	operon					1853:1858	homologous yebST operon	1836:1858	homologous yebST operon	1836:1858	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	75	theme	transport	1905:1913	arg1	proteins					1923:1930	transport pathway proteins	1905:1930	transport pathway proteins related to the Mla transport pathway	1905:1967	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	75	theme	transport	1905:1913	arg1	function					1888:1895	previously unknown function	1869:1895	previously unknown function	1869:1895	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	2	76	theme	bacteria	241:248	arg1	bilayer					267:273	an asymmetric bilayer	253:273	an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively	253:386	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	2	76	theme	bacteria	241:248	arg1	membrane					215:222	The outer membrane	205:222	The outer membrane of Gram-negative bacteria	205:248	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	12	77	theme	related	1517:1523	arg1	PqiABC					1471:1476	PqiABC	1471:1476	PqiABC	1471:1476	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	12	77	theme	related	1517:1523	arg1	pathways					1508:1515	novel transport pathways	1492:1515	novel transport pathways related to the Mla transport pathway and important for membrane integrity	1492:1589	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	12	77	theme	related	1517:1523	arg1	YebST					1482:1486	YebST	1482:1486	YebST	1482:1486	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	16	78	theme	mutant	2121:2126	arg1	strains					2128:2134	mla mutant strains	2117:2134	mla mutant strains	2117:2134	Deletion of these operons rendered cells more sensitive to membrane stress, and additional copies of pqiABC suppressed the SDS-EDTA sensitivity of mla mutant strains.
27795327	0	79	theme	coli	54:57	arg1	pqiABC					0:5	pqiABC	0:5	pqiABC	0:5	pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity.
27795327	0	79	theme	coli	54:57	arg1	Operons					31:37	Putative mce Operons	18:37	Putative mce Operons of Escherichia coli	18:57	pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity.
27795327	4	80	theme	transport	470:478	arg1	pathway					480:486	the Mla transport pathway	462:486	the Mla transport pathway	462:486	In Escherichia coli, the Mla transport pathway maintains this asymmetry by removing phospholipids from the outer leaflet.
27795327	11	81	theme	SDS-EDTA	1299:1306	arg1	sensitivity					1308:1318	the SDS-EDTA sensitivity	1295:1318	the SDS-EDTA sensitivity of mla mutants	1295:1333	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	1	82	theme	defense	196:202	arg1	line					188:191	the first line	178:191	the first line of defense	178:202	The membranes of single-cell organisms are crucial as the first line of defense.
27795327	11	83	theme	additional	1350:1359	arg1	copies					1361:1366	additional copies	1350:1366	additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules	1350:1453	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	5	84	theme	MCE	671:673	arg1	proteins					682:689	two other MCE domain proteins	661:689	two other MCE domain proteins of unknown function	661:709	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	5	85	theme	function	702:709	arg1	proteins					682:689	two other MCE domain proteins	661:689	two other MCE domain proteins of unknown function	661:709	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	5	86	theme	other	665:669	arg1	proteins					682:689	two other MCE domain proteins	661:689	two other MCE domain proteins of unknown function	661:709	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	15	87	theme	pathway	1915:1921	arg1	operon					1825:1830	the stress-inducible pqiABC operon	1797:1830	the stress-inducible pqiABC operon	1797:1830	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	87	theme	pathway	1915:1921	arg1	operon					1853:1858	homologous yebST operon	1836:1858	homologous yebST operon	1836:1858	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	87	theme	pathway	1915:1921	arg1	proteins					1923:1930	transport pathway proteins	1905:1930	transport pathway proteins related to the Mla transport pathway	1905:1967	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	87	theme	pathway	1915:1921	arg1	function					1888:1895	previously unknown function	1869:1895	previously unknown function	1869:1895	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	88	theme	unknown	1880:1886	arg1	operon					1825:1830	the stress-inducible pqiABC operon	1797:1830	the stress-inducible pqiABC operon	1797:1830	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	88	theme	unknown	1880:1886	arg1	operon					1853:1858	homologous yebST operon	1836:1858	homologous yebST operon	1836:1858	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	88	theme	unknown	1880:1886	arg1	proteins					1923:1930	transport pathway proteins	1905:1930	transport pathway proteins related to the Mla transport pathway	1905:1967	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	88	theme	unknown	1880:1886	arg1	function					1888:1895	previously unknown function	1869:1895	previously unknown function	1869:1895	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	12	89	theme	transport	1498:1506	arg1	PqiABC					1471:1476	PqiABC	1471:1476	PqiABC	1471:1476	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	12	89	theme	transport	1498:1506	arg1	pathways					1508:1515	novel transport pathways	1492:1515	novel transport pathways related to the Mla transport pathway and important for membrane integrity	1492:1589	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	12	89	theme	transport	1498:1506	arg1	YebST					1482:1486	YebST	1482:1486	YebST	1482:1486	In conclusion, PqiABC and YebST are novel transport pathways related to the Mla transport pathway and important for membrane integrity.
27795327	10	90	theme	transporter	1136:1146	arg1	proteins					1148:1155	transporter proteins	1136:1155	transporter proteins	1136:1155	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	5	91	theme	domain	675:680	arg1	proteins					682:689	two other MCE domain proteins	661:689	two other MCE domain proteins of unknown function	661:709	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	16	92	theme	additional	2050:2059	arg1	copies					2061:2066	additional copies	2050:2066	additional copies of pqiABC	2050:2076	Deletion of these operons rendered cells more sensitive to membrane stress, and additional copies of pqiABC suppressed the SDS-EDTA sensitivity of mla mutant strains.
27795327	5	93	theme	system	590:595	arg1	protein					636:642	a mammalian cell entry (MCE) domain protein	600:642	a mammalian cell entry (MCE) domain protein	600:642	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	5	93	theme	system	590:595	arg1	component					572:580	The MlaD component	563:580	The MlaD component of this system	563:595	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	2	94	theme	inner	360:364	arg1	leaflet					366:372	the outer and inner leaflet	346:372	the outer and inner leaflet	346:372	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	11	95	from	deletion	1189:1196	arg1	background					1237:1246	an Δmla background	1229:1246	an Δmla background	1229:1246	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
27795327	9	96	theme	operon	981:986	arg1	member					963:968	a third member	955:968	a third member of the pqi operon	955:986	Here, we found a third member of the pqi operon, ymbA (pqiC).
27795327	9	96	theme	operon	981:986	arg1	ymbA					989:992	ymbA	989:992	ymbA (pqiC)	989:999	Here, we found a third member of the pqi operon, ymbA (pqiC).
27795327	2	97	theme	outer	350:354	arg1	leaflet					366:372	the outer and inner leaflet	346:372	the outer and inner leaflet	346:372	The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharides (LPSs) and phospholipids are localized in the outer and inner leaflet, respectively.
27795327	5	98	theme	MlaD	567:570	arg1	protein					636:642	a mammalian cell entry (MCE) domain protein	600:642	a mammalian cell entry (MCE) domain protein	600:642	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	5	98	theme	MlaD	567:570	arg1	component					572:580	The MlaD component	563:580	The MlaD component of this system	563:595	The MlaD component of this system is a mammalian cell entry (MCE) domain protein, and E. coli has two other MCE domain proteins of unknown function (PqiB and YebT).
27795327	15	99	theme	yebST	1847:1851	arg1	operon					1825:1830	the stress-inducible pqiABC operon	1797:1830	the stress-inducible pqiABC operon	1797:1830	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	99	theme	yebST	1847:1851	arg1	operon					1853:1858	homologous yebST operon	1836:1858	homologous yebST operon	1836:1858	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	99	theme	yebST	1847:1851	arg1	proteins					1923:1930	transport pathway proteins	1905:1930	transport pathway proteins related to the Mla transport pathway	1905:1967	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	99	theme	yebST	1847:1851	arg1	function					1888:1895	previously unknown function	1869:1895	previously unknown function	1869:1895	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	4	100	theme	outer	548:552	arg1	leaflet					554:560	the outer leaflet	544:560	the outer leaflet	544:560	In Escherichia coli, the Mla transport pathway maintains this asymmetry by removing phospholipids from the outer leaflet.
27795327	15	101	theme	homologous	1836:1845	arg1	operon					1825:1830	the stress-inducible pqiABC operon	1797:1830	the stress-inducible pqiABC operon	1797:1830	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	101	theme	homologous	1836:1845	arg1	operon					1853:1858	homologous yebST operon	1836:1858	homologous yebST operon	1836:1858	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	101	theme	homologous	1836:1845	arg1	proteins					1923:1930	transport pathway proteins	1905:1930	transport pathway proteins related to the Mla transport pathway	1905:1967	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	101	theme	homologous	1836:1845	arg1	function					1888:1895	previously unknown function	1869:1895	previously unknown function	1869:1895	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	16	102	theme	SDS-EDTA	2093:2100	arg1	sensitivity					2102:2112	the SDS-EDTA sensitivity	2089:2112	the SDS-EDTA sensitivity of mla mutant strains	2089:2134	Deletion of these operons rendered cells more sensitive to membrane stress, and additional copies of pqiABC suppressed the SDS-EDTA sensitivity of mla mutant strains.
27795327	0	103	theme	mce	27:29	arg1	pqiABC					0:5	pqiABC	0:5	pqiABC	0:5	pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity.
27795327	0	103	theme	mce	27:29	arg1	Operons					31:37	Putative mce Operons	18:37	Putative mce Operons of Escherichia coli	18:57	pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity.
27795327	15	104	theme	Mla	1947:1949	arg1	pathway					1961:1967	the Mla transport pathway	1943:1967	the Mla transport pathway	1943:1967	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	17	105	with	strains	2194:2200	arg1	molecules					2221:2229	defective LPS molecules	2207:2229	defective LPS molecules	2207:2229	We found that yebS'-'lacZ fusion was activated in mutant strains with defective LPS molecules.
27795327	15	106	theme	stress-inducible	1801:1816	arg1	operon					1825:1830	the stress-inducible pqiABC operon	1797:1830	the stress-inducible pqiABC operon	1797:1830	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	106	theme	stress-inducible	1801:1816	arg1	operon					1853:1858	homologous yebST operon	1836:1858	homologous yebST operon	1836:1858	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	106	theme	stress-inducible	1801:1816	arg1	proteins					1923:1930	transport pathway proteins	1905:1930	transport pathway proteins related to the Mla transport pathway	1905:1967	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	15	106	theme	stress-inducible	1801:1816	arg1	function					1888:1895	previously unknown function	1869:1895	previously unknown function	1869:1895	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	10	107	theme	PqiB-PqiC	1004:1012	arg1	complex					1014:1020	A PqiB-PqiC complex	1002:1020	A PqiB-PqiC complex	1002:1020	A PqiB-PqiC complex bridges the inner and the outer membrane, and in other bacteria, pqiBC genes are located in operons together with transporter proteins.
27795327	14	108	theme	ABC	1758:1760	arg1	pathway					1772:1778	the Mla ABC transport pathway	1750:1778	the Mla ABC transport pathway	1750:1778	The composition of the E. coli outer membrane is asymmetric, with asymmetry maintained by the Mla ABC transport pathway.
27795327	16	109	theme	mla	2117:2119	arg1	strains					2128:2134	mla mutant strains	2117:2134	mla mutant strains	2117:2134	Deletion of these operons rendered cells more sensitive to membrane stress, and additional copies of pqiABC suppressed the SDS-EDTA sensitivity of mla mutant strains.
27795327	15	110	theme	transport	1951:1959	arg1	pathway					1961:1967	the Mla transport pathway	1943:1967	the Mla transport pathway	1943:1967	We propose that the stress-inducible pqiABC operon and homologous yebST operon, both of previously unknown function, encode transport pathway proteins related to the Mla transport pathway.
27795327	11	111	theme	Δmla	1232:1235	arg1	background					1237:1246	an Δmla background	1229:1246	an Δmla background	1229:1246	We show here that simultaneous deletion of pqiABC and yebST operons in an Δmla background rendered cells more sensitive to SDS-EDTA, and the SDS-EDTA sensitivity of mla mutants was rescued by additional copies of pqiABC We also found that the yebST operon was induced by a defect in LPS molecules.
26610691	2	0	theme	building	521:528	arg1	blocks					530:535	building blocks	521:535	building blocks to reconstruct a new tissue in vitro or in vivo	521:583	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	2	0	theme	building	521:528	arg1	components					507:516	cell-loaded micrometer-scale modular components	470:516	cell-loaded micrometer-scale modular components	470:516	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	8	1	theme	3D	1652:1653	arg1	composite					1691:1699	a macroscopic 3D geometrically shaped cartilage-like composite	1638:1699	a macroscopic 3D geometrically shaped cartilage-like composite	1638:1699	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	4	2	theme	promising	938:946	arg1	application					948:958	promising application	938:958	promising application potential for TE	938:975	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	4	2	theme	promising	938:946	arg1	regeneration					1010:1021	the cartilage tissue regeneration	989:1021	the cartilage tissue regeneration	989:1021	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	0	3	theme	building	99:106	arg1	blocks					108:113	cell-carrier building blocks	86:113	cell-carrier building blocks for bottom-up cartilage tissue engineering	86:156	Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering.
26610691	2	4	theme	bulk	679:682	arg1	approach					711:718	the traditional bulk scaffold based top-down TE approach	663:718	the traditional bulk scaffold based top-down TE approach	663:718	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	6	5	theme	controlled	1325:1334	arg1	microstructure					1348:1361	controlled nanofibrous microstructure	1325:1361	controlled nanofibrous microstructure	1325:1361	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	8	6	theme	cartilage	1882:1890	arg1	engineering					1899:1909	cartilage tissue engineering	1882:1909	cartilage tissue engineering	1882:1909	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	0	7	theme	bottom-up	119:127	arg1	engineering					146:156	bottom-up cartilage tissue engineering	119:156	bottom-up cartilage tissue engineering	119:156	Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering.
26610691	5	8	theme	physical	1184:1191	arg1	process					1202:1208	a physical gelation process	1182:1208	a physical gelation process	1182:1208	According to this background, we report here the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process.
26610691	4	9	theme	intrinsic	913:921	arg1	properties					923:932	appealing intrinsic properties	903:932	appealing intrinsic properties	903:932	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	2	10	theme	traditional	667:677	arg1	approach					711:718	the traditional bulk scaffold based top-down TE approach	663:718	the traditional bulk scaffold based top-down TE approach	663:718	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	6	11	theme	fabrication	1238:1248	arg1	procedure					1250:1258	this physical fabrication procedure	1224:1258	this physical fabrication procedure	1224:1258	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	2	12	theme	new	554:556	arg1	tissue					558:563	a new tissue	552:563	a new tissue	552:563	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	1	13	theme	new	377:379	arg1	formation					388:396	new tissue formation	377:396	new tissue formation	377:396	Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation.
26610691	4	14	theme	appealing	903:911	arg1	properties					923:932	appealing intrinsic properties	903:932	appealing intrinsic properties	903:932	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	6	15	theme	physical	1229:1236	arg1	procedure					1250:1258	this physical fabrication procedure	1224:1258	this physical fabrication procedure	1224:1258	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	8	16	theme	application	1807:1817	arg1	potential					1819:1827	significant application potential	1795:1827	significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering	1795:1909	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	8	17	theme	nanofibrous	1736:1746	arg1	NCMS					1753:1756	NCMS	1753:1756	NCMS	1753:1756	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	8	17	theme	nanofibrous	1736:1746	arg1	CMS					1748:1750	the nanofibrous CMS	1732:1750	the nanofibrous CMS (NCMS)	1732:1757	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	0	18	theme	tissue	139:144	arg1	engineering					146:156	bottom-up cartilage tissue engineering	119:156	bottom-up cartilage tissue engineering	119:156	Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering.
26610691	1	19	theme	tissue	381:386	arg1	formation					388:396	new tissue formation	377:396	new tissue formation	377:396	Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation.
26610691	4	20	theme	natural	869:875	arg1	polymer					877:883	an accessible natural polymer	855:883	an accessible natural polymer	855:883	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	4	20	theme	natural	869:875	arg1	Chitosan					840:847	Chitosan	840:847	Chitosan (CS)	840:852	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	8	21	theme	chondrocyte	1536:1546	arg1	tests					1556:1560	in vitro chondrocyte culture tests	1527:1560	in vitro chondrocyte culture tests	1527:1560	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	8	22	theme	significant	1795:1805	arg1	potential					1819:1827	significant application potential	1795:1827	significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering	1795:1909	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	0	23	theme	cartilage	129:137	arg1	engineering					146:156	bottom-up cartilage tissue engineering	119:156	bottom-up cartilage tissue engineering	119:156	Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering.
26610691	5	24	theme	first	1162:1166	arg1	time					1168:1171	the first time	1158:1171	the first time based on a physical gelation process	1158:1208	According to this background, we report here the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process.
26610691	2	25	theme	advantages	638:647	arg1	number					618:623	a number	616:623	a number of desirable advantages	616:647	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	2	26	theme	TE	708:709	arg1	approach					711:718	the traditional bulk scaffold based top-down TE approach	663:718	the traditional bulk scaffold based top-down TE approach	663:718	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	7	27	theme	toxic	1434:1438	arg1	agent					1478:1482	no potentially toxic or denaturizing chemical crosslinking agent	1419:1482	no potentially toxic or denaturizing chemical crosslinking agent	1419:1482	Especially, no potentially toxic or denaturizing chemical crosslinking agent was introduced into the products.
26610691	4	28	theme	tissue	1003:1008	arg1	application					948:958	promising application	938:958	promising application potential for TE	938:975	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	4	28	theme	tissue	1003:1008	arg1	regeneration					1010:1021	the cartilage tissue regeneration	989:1021	the cartilage tissue regeneration	989:1021	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	8	29	theme	shaped	1669:1674	arg1	composite					1691:1699	a macroscopic 3D geometrically shaped cartilage-like composite	1638:1699	a macroscopic 3D geometrically shaped cartilage-like composite	1638:1699	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	2	30	theme	desirable	628:636	arg1	advantages					638:647	desirable advantages	628:647	desirable advantages	628:647	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	2	31	theme	top-down	699:706	arg1	approach					711:718	the traditional bulk scaffold based top-down TE approach	663:718	the traditional bulk scaffold based top-down TE approach	663:718	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	6	32	theme	tunable	1367:1373	arg1	sizes					1375:1379	tunable sizes	1367:1379	tunable sizes	1367:1379	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	3	33	theme	ECM-mimicking	760:772	arg1	structure					786:794	an ECM-mimicking nanofibrous structure	757:794	an ECM-mimicking nanofibrous structure	757:794	Nevertheless, micro-components with an ECM-mimicking nanofibrous structure are still very scarce and highly desirable.
26610691	0	34	theme	Chitosan	0:7	arg1	microspheres					9:20	Chitosan microspheres	0:20	Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering.	0:157	Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering.
26610691	2	35	theme	based	693:697	arg1	approach					711:718	the traditional bulk scaffold based top-down TE approach	663:718	the traditional bulk scaffold based top-down TE approach	663:718	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	2	36	theme	TE	448:449	arg1	approach					451:458	a bottom-up TE approach	436:458	a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo	436:583	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	2	37	theme	scaffold	684:691	arg1	approach					711:718	the traditional bulk scaffold based top-down TE approach	663:718	the traditional bulk scaffold based top-down TE approach	663:718	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	8	38	theme	NCMS	1832:1835	arg1	potential					1819:1827	significant application potential	1795:1827	significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering	1795:1909	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	2	39	theme	bottom-up	438:446	arg1	approach					451:458	a bottom-up TE approach	436:458	a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo	436:583	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	3	40	theme	nanofibrous	774:784	arg1	structure					786:794	an ECM-mimicking nanofibrous structure	757:794	an ECM-mimicking nanofibrous structure	757:794	Nevertheless, micro-components with an ECM-mimicking nanofibrous structure are still very scarce and highly desirable.
26610691	4	41	theme	potential	960:968	arg1	application					948:958	promising application	938:958	promising application potential for TE	938:975	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	4	41	theme	potential	960:968	arg1	regeneration					1010:1021	the cartilage tissue regeneration	989:1021	the cartilage tissue regeneration	989:1021	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	8	42	theme	in	1527:1528	arg1	tests					1556:1560	in vitro chondrocyte culture tests	1527:1560	in vitro chondrocyte culture tests	1527:1560	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	8	43	theme	bottom-up	1844:1852	arg1	components					1867:1876	the bottom-up cell-carrier components	1840:1876	the bottom-up cell-carrier components	1840:1876	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	1	44	theme	physicochemical	221:235	arg1	properties					237:246	the physicochemical properties	217:246	the physicochemical properties of the natural extracellular matrix (ECM)	217:288	Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation.
26610691	0	45	theme	matrix-mimicking	44:59	arg1	structure					73:81	an extracellular matrix-mimicking nanofibrous structure	27:81	an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering	27:156	Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering.
26610691	8	46	theme	macroscopic	1640:1650	arg1	composite					1691:1699	a macroscopic 3D geometrically shaped cartilage-like composite	1638:1699	a macroscopic 3D geometrically shaped cartilage-like composite	1638:1699	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	8	47	dep	in	1527:1528	arg1	vitro					1530:1534	vitro	1530:1534	vitro	1530:1534	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	5	48	theme	ECM-mimicking	1118:1130	arg1	structure					1144:1152	an ECM-mimicking nanofibrous structure	1115:1152	an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process	1115:1208	According to this background, we report here the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process.
26610691	7	49	theme	chemical	1456:1463	arg1	agent					1478:1482	no potentially toxic or denaturizing chemical crosslinking agent	1419:1482	no potentially toxic or denaturizing chemical crosslinking agent	1419:1482	Especially, no potentially toxic or denaturizing chemical crosslinking agent was introduced into the products.
26610691	5	50	with	fabrication	1073:1083	arg1	structure					1144:1152	an ECM-mimicking nanofibrous structure	1115:1152	an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process	1115:1208	According to this background, we report here the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process.
26610691	0	51	theme	extracellular	30:42	arg1	structure					73:81	an extracellular matrix-mimicking nanofibrous structure	27:81	an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering	27:156	Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering.
26610691	5	52	theme	gelation	1193:1200	arg1	process					1202:1208	a physical gelation process	1182:1208	a physical gelation process	1182:1208	According to this background, we report here the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process.
26610691	2	53	theme	modular	499:505	arg1	blocks					530:535	building blocks	521:535	building blocks to reconstruct a new tissue in vitro or in vivo	521:583	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	2	53	theme	modular	499:505	arg1	components					507:516	cell-loaded micrometer-scale modular components	470:516	cell-loaded micrometer-scale modular components	470:516	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	5	54	theme	nanofibrous	1132:1142	arg1	structure					1144:1152	an ECM-mimicking nanofibrous structure	1115:1152	an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process	1115:1208	According to this background, we report here the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process.
26610691	6	55	theme	CS	1298:1299	arg1	CMS					1315:1317	CMS	1315:1317	CMS	1315:1317	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	6	55	theme	CS	1298:1299	arg1	microspheres					1301:1312	uniform CS microspheres	1290:1312	uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes	1290:1379	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	7	56	theme	denaturizing	1443:1454	arg1	agent					1478:1482	no potentially toxic or denaturizing chemical crosslinking agent	1419:1482	no potentially toxic or denaturizing chemical crosslinking agent	1419:1482	Especially, no potentially toxic or denaturizing chemical crosslinking agent was introduced into the products.
26610691	2	57	theme	valuable	414:421	arg1	alternative					423:433	a valuable alternative	412:433	a valuable alternative	412:433	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	2	58	theme	micrometer-scale	482:497	arg1	blocks					530:535	building blocks	521:535	building blocks to reconstruct a new tissue in vitro or in vivo	521:583	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	2	58	theme	micrometer-scale	482:497	arg1	components					507:516	cell-loaded micrometer-scale modular components	470:516	cell-loaded micrometer-scale modular components	470:516	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	1	59	theme	cell	331:334	arg1	attachment					336:345	cell attachment	331:345	cell attachment	331:345	Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation.
26610691	6	60	theme	uniform	1290:1296	arg1	CMS					1315:1317	CMS	1315:1317	CMS	1315:1317	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	6	60	theme	uniform	1290:1296	arg1	microspheres					1301:1312	uniform CS microspheres	1290:1312	uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes	1290:1379	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	8	61	theme	cell-carrier	1854:1865	arg1	components					1867:1876	the bottom-up cell-carrier components	1840:1876	the bottom-up cell-carrier components	1840:1876	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	0	62	theme	nanofibrous	61:71	arg1	structure					73:81	an extracellular matrix-mimicking nanofibrous structure	27:81	an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering	27:156	Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering.
26610691	6	63	with	microspheres	1301:1312	arg1	microstructure					1348:1361	controlled nanofibrous microstructure	1325:1361	controlled nanofibrous microstructure	1325:1361	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	6	63	with	microspheres	1301:1312	arg1	sizes					1375:1379	tunable sizes	1367:1379	tunable sizes	1367:1379	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	2	64	theme	cell-loaded	470:480	arg1	blocks					530:535	building blocks	521:535	building blocks to reconstruct a new tissue in vitro or in vivo	521:583	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	2	64	theme	cell-loaded	470:480	arg1	components					507:516	cell-loaded micrometer-scale modular components	470:516	cell-loaded micrometer-scale modular components	470:516	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	4	65	theme	accessible	858:867	arg1	polymer					877:883	an accessible natural polymer	855:883	an accessible natural polymer	855:883	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	4	65	theme	accessible	858:867	arg1	Chitosan					840:847	Chitosan	840:847	Chitosan (CS)	840:852	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	0	66	with	microspheres	9:20	arg1	structure					73:81	an extracellular matrix-mimicking nanofibrous structure	27:81	an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering	27:156	Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering.
26610691	6	67	theme	nanofibrous	1336:1346	arg1	microstructure					1348:1361	controlled nanofibrous microstructure	1325:1361	controlled nanofibrous microstructure	1325:1361	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	3	68	with	micro-components	735:750	arg1	structure					786:794	an ECM-mimicking nanofibrous structure	757:794	an ECM-mimicking nanofibrous structure	757:794	Nevertheless, micro-components with an ECM-mimicking nanofibrous structure are still very scarce and highly desirable.
26610691	8	69	theme	cartilage-like	1676:1689	arg1	composite					1691:1699	a macroscopic 3D geometrically shaped cartilage-like composite	1638:1699	a macroscopic 3D geometrically shaped cartilage-like composite	1638:1699	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	5	70	theme	chitosan	1088:1095	arg1	microspheres					1097:1108	chitosan microspheres	1088:1108	chitosan microspheres	1088:1108	According to this background, we report here the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process.
26610691	7	71	theme	crosslinking	1465:1476	arg1	agent					1478:1482	no potentially toxic or denaturizing chemical crosslinking agent	1419:1482	no potentially toxic or denaturizing chemical crosslinking agent	1419:1482	Especially, no potentially toxic or denaturizing chemical crosslinking agent was introduced into the products.
26610691	2	72	dep	blocks	530:535	arg1	reconstruct					540:550	reconstruct	540:550	to reconstruct a new tissue in vitro or in vivo	537:583	Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach.
26610691	5	73	theme	microspheres	1097:1108	arg1	fabrication					1073:1083	the fabrication	1069:1083	the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process	1069:1208	According to this background, we report here the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process.
26610691	6	74	theme	microfluidic	1265:1276	arg1	technology					1278:1287	microfluidic technology	1265:1287	microfluidic technology	1265:1287	By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained.
26610691	8	75	theme	enhanced	1576:1583	arg1	attachment					1590:1599	enhanced cell attachment	1576:1599	enhanced cell attachment	1576:1599	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	8	76	theme	culture	1548:1554	arg1	tests					1556:1560	in vitro chondrocyte culture tests	1527:1560	in vitro chondrocyte culture tests	1527:1560	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	1	77	theme	natural	255:261	arg1	ECM					285:287	ECM	285:287	ECM	285:287	Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation.
26610691	1	77	theme	natural	255:261	arg1	matrix					277:282	the natural extracellular matrix	251:282	the natural extracellular matrix (ECM)	251:288	Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation.
26610691	0	78	theme	cell-carrier	86:97	arg1	blocks					108:113	cell-carrier building blocks	86:113	cell-carrier building blocks for bottom-up cartilage tissue engineering	86:156	Chitosan microspheres with an extracellular matrix-mimicking nanofibrous structure as cell-carrier building blocks for bottom-up cartilage tissue engineering.
26610691	8	79	theme	tissue	1892:1897	arg1	engineering					1899:1909	cartilage tissue engineering	1882:1909	cartilage tissue engineering	1882:1909	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	1	80	theme	tissue	173:178	arg1	engineering					180:190	tissue engineering	173:190	tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM)	173:288	Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation.
26610691	1	80	theme	tissue	173:178	arg1	TE					193:194	TE	193:194	TE	193:194	Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation.
26610691	1	81	theme	extracellular	263:275	arg1	ECM					285:287	ECM	285:287	ECM	285:287	Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation.
26610691	1	81	theme	extracellular	263:275	arg1	matrix					277:282	the natural extracellular matrix	251:282	the natural extracellular matrix (ECM)	251:288	Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation.
26610691	8	82	theme	cell	1585:1588	arg1	attachment					1590:1599	enhanced cell attachment	1576:1599	enhanced cell attachment	1576:1599	Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.
26610691	4	83	theme	cartilage	993:1001	arg1	application					948:958	promising application	938:958	promising application potential for TE	938:975	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	4	83	theme	cartilage	993:1001	arg1	regeneration					1010:1021	the cartilage tissue regeneration	989:1021	the cartilage tissue regeneration	989:1021	Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration.
26610691	1	84	theme	matrix	277:282	arg1	properties					237:246	the physicochemical properties	217:246	the physicochemical properties of the natural extracellular matrix (ECM)	217:288	Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation.
27959566	4	0	theme	produced	699:706	arg1	CNCs					708:711	industrially produced CNCs	686:711	industrially produced CNCs	686:711	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	1	theme	procedures	788:797	arg1	sources					742:748	cellulose sources	732:748	cellulose sources	732:748	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	1	theme	procedures	788:797	arg1	methods					762:768	hydrolysis methods	751:768	hydrolysis methods	751:768	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	1	theme	procedures	788:797	arg1	procedures					788:797	purification procedures	775:797	purification procedures	775:797	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	1	theme	procedures	788:797	arg1	CNCs					708:711	industrially produced CNCs	686:711	industrially produced CNCs	686:711	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	1	theme	procedures	788:797	arg1	variety					721:727	the variety	717:727	the variety of cellulose sources, hydrolysis methods, and purification procedures	717:797	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	5	2	theme	CNC	952:954	arg1	dependent					990:998	dependent	990:998	dependent	990:998	This has further been justified by the past two decades of research that demonstrate that the CNC stability and behavior are highly dependent on the surface chemistry, surface charge density, and particle size.
27959566	5	2	theme	CNC	952:954	arg1	stability					956:964	the CNC stability	948:964	the CNC stability	948:964	This has further been justified by the past two decades of research that demonstrate that the CNC stability and behavior are highly dependent on the surface chemistry, surface charge density, and particle size.
27959566	5	3	theme	past	897:900	arg1	decades					906:912	the past two decades	893:912	the past two decades of research that demonstrate that the CNC stability and behavior are highly dependent on the surface chemistry, surface charge density, and particle size	893:1066	This has further been justified by the past two decades of research that demonstrate that the CNC stability and behavior are highly dependent on the surface chemistry, surface charge density, and particle size.
27959566	2	4	theme	commercial	340:349	arg1	applications					351:362	commercial applications	340:362	commercial applications	340:362	However, for CNCs to shift from a laboratory curiosity to commercial applications, researchers must transition from CNCs extracted on the bench scale to material produced on an industrial scale.
27959566	8	5	theme	further	1503:1509	arg1	purification					1511:1522	further purification	1503:1522	further purification of CNCs using Soxhlet extraction in ethanol	1503:1566	In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications.
27959566	3	6	theme	nanocelluloses	614:627	arg1	quantities					553:562	day quantities	549:562	day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses	549:627	There are a number of companies currently producing kilogram to ton per day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses, as described herein.
27959566	4	7	theme	materials	830:838	arg1	characterization					804:819	the characterization	800:819	the characterization of these materials	800:838	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	8	theme	methods	762:768	arg1	sources					742:748	cellulose sources	732:748	cellulose sources	732:748	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	8	theme	methods	762:768	arg1	methods					762:768	hydrolysis methods	751:768	hydrolysis methods	751:768	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	8	theme	methods	762:768	arg1	procedures					788:797	purification procedures	775:797	purification procedures	775:797	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	8	theme	methods	762:768	arg1	CNCs					708:711	industrially produced CNCs	686:711	industrially produced CNCs	686:711	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	8	theme	methods	762:768	arg1	variety					721:727	the variety	717:727	the variety of cellulose sources, hydrolysis methods, and purification procedures	717:797	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	9	theme	sources	742:748	arg1	sources					742:748	cellulose sources	732:748	cellulose sources	732:748	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	9	theme	sources	742:748	arg1	methods					762:768	hydrolysis methods	751:768	hydrolysis methods	751:768	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	9	theme	sources	742:748	arg1	procedures					788:797	purification procedures	775:797	purification procedures	775:797	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	9	theme	sources	742:748	arg1	CNCs					708:711	industrially produced CNCs	686:711	industrially produced CNCs	686:711	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	9	theme	sources	742:748	arg1	variety					721:727	the variety	717:727	the variety of cellulose sources, hydrolysis methods, and purification procedures	717:797	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	1	10	theme	rheological	256:266	arg1	applications					268:279	numerous composite, biomedical, and rheological applications	220:279	numerous composite, biomedical, and rheological applications	220:279	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
27959566	7	11	theme	acid-extracted	1244:1257	arg1	CNCs					1259:1262	the sulfuric acid-extracted CNCs	1231:1262	the sulfuric acid-extracted CNCs examined	1231:1271	Of the sulfuric acid-extracted CNCs examined, industrially produced material compared well with laboratory-made CNCs, exhibiting similar charge density, colloidal and thermal stability, crystallinity, morphology, and self-assembly behavior.
27959566	8	12	theme	CNCs	1527:1530	arg1	purification					1511:1522	further purification	1503:1522	further purification of CNCs using Soxhlet extraction in ethanol	1503:1566	In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications.
27959566	8	13	from	extraction	1546:1555	arg1	ethanol					1560:1566	ethanol	1560:1566	ethanol	1560:1566	In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications.
27959566	4	14	theme	hydrolysis	751:760	arg1	methods					762:768	hydrolysis methods	751:768	hydrolysis methods	751:768	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	8	15	located	observed	1489:1496	arg2	it					1482:1483	it	1482:1483	it	1482:1483	In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications.
27959566	8	15	located	observed	1489:1496	arg1	addition					1472:1479	addition	1472:1479	addition	1472:1479	In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications.
27959566	7	16	theme	colloidal	1381:1389	arg1	stability					1403:1411	colloidal and thermal stability	1381:1411	stability	1403:1411	Of the sulfuric acid-extracted CNCs examined, industrially produced material compared well with laboratory-made CNCs, exhibiting similar charge density, colloidal and thermal stability, crystallinity, morphology, and self-assembly behavior.
27959566	5	17	theme	research	917:924	arg1	decades					906:912	the past two decades	893:912	the past two decades of research that demonstrate that the CNC stability and behavior are highly dependent on the surface chemistry, surface charge density, and particle size	893:1066	This has further been justified by the past two decades of research that demonstrate that the CNC stability and behavior are highly dependent on the surface chemistry, surface charge density, and particle size.
27959566	7	18	theme	laboratory-made	1324:1338	arg1	CNCs					1340:1343	laboratory-made CNCs	1324:1343	laboratory-made CNCs	1324:1343	Of the sulfuric acid-extracted CNCs examined, industrially produced material compared well with laboratory-made CNCs, exhibiting similar charge density, colloidal and thermal stability, crystallinity, morphology, and self-assembly behavior.
27959566	7	19	theme	self-assembly	1445:1457	arg1	behavior					1459:1466	self-assembly behavior	1445:1466	self-assembly behavior	1445:1466	Of the sulfuric acid-extracted CNCs examined, industrially produced material compared well with laboratory-made CNCs, exhibiting similar charge density, colloidal and thermal stability, crystallinity, morphology, and self-assembly behavior.
27959566	7	20	theme	charge	1365:1370	arg1	density					1372:1378	similar charge density	1357:1378	similar charge density	1357:1378	Of the sulfuric acid-extracted CNCs examined, industrially produced material compared well with laboratory-made CNCs, exhibiting similar charge density, colloidal and thermal stability, crystallinity, morphology, and self-assembly behavior.
27959566	4	21	theme	purification	775:786	arg1	procedures					788:797	purification procedures	775:797	purification procedures	775:797	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	0	22	theme	Cellulose	13:21	arg1	Nanocrystals					23:34	Benchmarking Cellulose Nanocrystals	0:34	Benchmarking Cellulose Nanocrystals: From the Laboratory to Industrial Production.	0:81	Benchmarking Cellulose Nanocrystals: From the Laboratory to Industrial Production.
27959566	4	23	theme	cellulose	732:740	arg1	sources					742:748	cellulose sources	732:748	cellulose sources	732:748	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	4	24	theme	recent	660:665	arg1	intensification					667:681	the recent intensification	656:681	the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures	656:797	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	0	25	theme	Benchmarking	0:11	arg1	Nanocrystals					23:34	Benchmarking Cellulose Nanocrystals	0:34	Benchmarking Cellulose Nanocrystals: From the Laboratory to Industrial Production.	0:81	Benchmarking Cellulose Nanocrystals: From the Laboratory to Industrial Production.
27959566	9	26	theme	current	1686:1692	arg1	standing					1694:1701	the current standing	1682:1701	the current standing of industrially produced CNCs	1682:1731	Overall, the current standing of industrially produced CNCs is positive, suggesting that the evolution to commercial-scale applications will not be hindered by CNC production.
27959566	9	26	theme	current	1686:1692	arg1	positive					1736:1743	positive	1736:1743	positive	1736:1743	Overall, the current standing of industrially produced CNCs is positive, suggesting that the evolution to commercial-scale applications will not be hindered by CNC production.
27959566	7	27	theme	similar	1357:1363	arg1	density					1372:1378	similar charge density	1357:1378	similar charge density	1357:1378	Of the sulfuric acid-extracted CNCs examined, industrially produced material compared well with laboratory-made CNCs, exhibiting similar charge density, colloidal and thermal stability, crystallinity, morphology, and self-assembly behavior.
27959566	9	28	theme	commercial-scale	1779:1794	arg1	applications					1796:1807	commercial-scale applications	1779:1807	commercial-scale applications	1779:1807	Overall, the current standing of industrially produced CNCs is positive, suggesting that the evolution to commercial-scale applications will not be hindered by CNC production.
27959566	5	29	theme	surface	1026:1032	arg1	density					1041:1047	surface charge density	1026:1047	surface charge density	1026:1047	This has further been justified by the past two decades of research that demonstrate that the CNC stability and behavior are highly dependent on the surface chemistry, surface charge density, and particle size.
27959566	7	30	theme	thermal	1395:1401	arg1	stability					1403:1411	colloidal and thermal stability	1381:1411	stability	1403:1411	Of the sulfuric acid-extracted CNCs examined, industrially produced material compared well with laboratory-made CNCs, exhibiting similar charge density, colloidal and thermal stability, crystallinity, morphology, and self-assembly behavior.
27959566	5	31	theme	surface	1007:1013	arg1	chemistry					1015:1023	the surface chemistry	1003:1023	the surface chemistry	1003:1023	This has further been justified by the past two decades of research that demonstrate that the CNC stability and behavior are highly dependent on the surface chemistry, surface charge density, and particle size.
27959566	2	32	theme	bench	420:424	arg1	scale					426:430	the bench scale	416:430	the bench scale to material produced on an industrial scale	416:474	However, for CNCs to shift from a laboratory curiosity to commercial applications, researchers must transition from CNCs extracted on the bench scale to material produced on an industrial scale.
27959566	8	33	theme	many	1654:1657	arg1	applications					1659:1670	many applications	1654:1670	many applications	1654:1670	In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications.
27959566	5	34	theme	particle	1054:1061	arg1	size					1063:1066	particle size	1054:1066	particle size	1054:1066	This has further been justified by the past two decades of research that demonstrate that the CNC stability and behavior are highly dependent on the surface chemistry, surface charge density, and particle size.
27959566	3	35	theme	day	549:551	arg1	quantities					553:562	day quantities	549:562	day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses	549:627	There are a number of companies currently producing kilogram to ton per day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses, as described herein.
27959566	1	36	theme	attractive	196:205	arg1	material					207:214	an attractive material	193:214	them an attractive material for numerous composite, biomedical, and rheological applications	188:279	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
27959566	9	37	theme	CNC	1833:1835	arg1	production					1837:1846	CNC production	1833:1846	CNC production	1833:1846	Overall, the current standing of industrially produced CNCs is positive, suggesting that the evolution to commercial-scale applications will not be hindered by CNC production.
27959566	4	38	theme	CNCs	708:711	arg1	intensification					667:681	the recent intensification	656:681	the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures	656:797	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	3	39	theme	CNCs	592:595	arg1	quantities					553:562	day quantities	549:562	day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses	549:627	There are a number of companies currently producing kilogram to ton per day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses, as described herein.
27959566	2	40	theme	industrial	459:468	arg1	scale					470:474	an industrial scale	456:474	an industrial scale	456:474	However, for CNCs to shift from a laboratory curiosity to commercial applications, researchers must transition from CNCs extracted on the bench scale to material produced on an industrial scale.
27959566	9	41	theme	produced	1719:1726	arg1	CNCs					1728:1731	industrially produced CNCs	1706:1731	industrially produced CNCs	1706:1731	Overall, the current standing of industrially produced CNCs is positive, suggesting that the evolution to commercial-scale applications will not be hindered by CNC production.
27959566	1	42	dep	them	188:191	arg1	material					207:214	an attractive material	193:214	them an attractive material for numerous composite, biomedical, and rheological applications	188:279	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
27959566	7	43	theme	produced	1287:1294	arg1	material					1296:1303	industrially produced material	1274:1303	industrially produced material	1274:1303	Of the sulfuric acid-extracted CNCs examined, industrially produced material compared well with laboratory-made CNCs, exhibiting similar charge density, colloidal and thermal stability, crystallinity, morphology, and self-assembly behavior.
27959566	8	44	theme	minimal	1572:1578	arg1	impact					1580:1585	minimal impact	1572:1585	minimal impact	1572:1585	In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications.
27959566	9	45	theme	CNCs	1728:1731	arg1	standing					1694:1701	the current standing	1682:1701	the current standing of industrially produced CNCs	1682:1731	Overall, the current standing of industrially produced CNCs is positive, suggesting that the evolution to commercial-scale applications will not be hindered by CNC production.
27959566	9	45	theme	CNCs	1728:1731	arg1	positive					1736:1743	positive	1736:1743	positive	1736:1743	Overall, the current standing of industrially produced CNCs is positive, suggesting that the evolution to commercial-scale applications will not be hindered by CNC production.
27959566	3	46	theme	sulfuric	567:574	arg1	CNCs					592:595	sulfuric acid-hydrolyzed CNCs	567:595	sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses	567:627	There are a number of companies currently producing kilogram to ton per day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses, as described herein.
27959566	6	47	theme	test	1092:1095	arg1	methods					1097:1103	key test methods	1088:1103	key test methods that should be employed to characterize these properties to ensure a "known" starting material and consistent performance	1088:1225	This work outlines key test methods that should be employed to characterize these properties to ensure a "known" starting material and consistent performance.
27959566	8	48	theme	Soxhlet	1538:1544	arg1	extraction					1546:1555	Soxhlet extraction	1538:1555	Soxhlet extraction in ethanol	1538:1566	In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications.
27959566	5	49	theme	charge	1034:1039	arg1	density					1041:1047	surface charge density	1026:1047	surface charge density	1026:1047	This has further been justified by the past two decades of research that demonstrate that the CNC stability and behavior are highly dependent on the surface chemistry, surface charge density, and particle size.
27959566	3	50	theme	acid-hydrolyzed	576:590	arg1	CNCs					592:595	sulfuric acid-hydrolyzed CNCs	567:595	sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses	567:627	There are a number of companies currently producing kilogram to ton per day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses, as described herein.
27959566	2	51	from	CNCs	398:401	arg1	transition					382:391	transition	382:391	transition	382:391	However, for CNCs to shift from a laboratory curiosity to commercial applications, researchers must transition from CNCs extracted on the bench scale to material produced on an industrial scale.
27959566	1	52	theme	mechanical	123:132	arg1	properties					134:143	mechanical properties	123:143	mechanical properties of cellulose nanocrystals (CNCs)	123:176	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
27959566	4	53	theme	variety	721:727	arg1	intensification					667:681	the recent intensification	656:681	the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures	656:797	With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical.
27959566	7	54	theme	sulfuric	1235:1242	arg1	CNCs					1259:1262	the sulfuric acid-extracted CNCs	1231:1262	the sulfuric acid-extracted CNCs examined	1231:1271	Of the sulfuric acid-extracted CNCs examined, industrially produced material compared well with laboratory-made CNCs, exhibiting similar charge density, colloidal and thermal stability, crystallinity, morphology, and self-assembly behavior.
27959566	1	55	theme	cellulose	148:156	arg1	nanocrystals					158:169	cellulose nanocrystals	148:169	cellulose nanocrystals (CNCs)	148:176	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
27959566	1	55	theme	cellulose	148:156	arg1	CNCs					172:175	CNCs	172:175	CNCs	172:175	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
27959566	3	56	theme	other	608:612	arg1	nanocelluloses					614:627	other nanocelluloses	608:627	sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses	567:627	There are a number of companies currently producing kilogram to ton per day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses, as described herein.
27959566	6	57	theme	consistent	1204:1213	arg1	performance					1215:1225	consistent performance	1204:1225	consistent performance	1204:1225	This work outlines key test methods that should be employed to characterize these properties to ensure a "known" starting material and consistent performance.
27959566	2	58	theme	laboratory	316:325	arg1	curiosity					327:335	a laboratory curiosity	314:335	a laboratory curiosity to commercial applications	314:362	However, for CNCs to shift from a laboratory curiosity to commercial applications, researchers must transition from CNCs extracted on the bench scale to material produced on an industrial scale.
27959566	1	59	theme	numerous	220:227	arg1	applications					268:279	numerous composite, biomedical, and rheological applications	220:279	numerous composite, biomedical, and rheological applications	220:279	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
27959566	0	60	dep	Nanocrystals	23:34	arg1	Laboratory					46:55	the Laboratory	42:55	the Laboratory	42:55	Benchmarking Cellulose Nanocrystals: From the Laboratory to Industrial Production.
27959566	6	61	theme	key	1088:1090	arg1	methods					1097:1103	key test methods	1088:1103	key test methods that should be employed to characterize these properties to ensure a "known" starting material and consistent performance	1088:1225	This work outlines key test methods that should be employed to characterize these properties to ensure a "known" starting material and consistent performance.
27959566	1	62	theme	composite	229:237	arg1	applications					268:279	numerous composite, biomedical, and rheological applications	220:279	numerous composite, biomedical, and rheological applications	220:279	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
27959566	6	63	theme	known	1175:1179	arg1	"					1180:1180	a "known"	1172:1180	a "known" starting material and consistent performance	1172:1225	This work outlines key test methods that should be employed to characterize these properties to ensure a "known" starting material and consistent performance.
27959566	8	64	contain	had	1568:1570	arg2	impact					1580:1585	minimal impact	1572:1585	minimal impact	1572:1585	In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications.
27959566	8	64	contain	had	1568:1570	arg1	purification					1511:1522	further purification	1503:1522	further purification of CNCs using Soxhlet extraction in ethanol	1503:1566	In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications.
27959566	3	65	theme	companies	499:507	arg1	number					489:494	a number	487:494	a number of companies currently producing kilogram to ton per day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses	487:627	There are a number of companies currently producing kilogram to ton per day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses, as described herein.
27959566	0	66	theme	Industrial	60:69	arg1	Production					71:80	Industrial Production	60:80	Industrial Production	60:80	Benchmarking Cellulose Nanocrystals: From the Laboratory to Industrial Production.
27959566	8	67	theme	nanoparticle	1594:1605	arg1	properties					1607:1616	the nanoparticle properties	1590:1616	the nanoparticle properties	1590:1616	In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications.
27959566	1	68	theme	nanocrystals	158:169	arg1	properties					134:143	mechanical properties	123:143	mechanical properties of cellulose nanocrystals (CNCs)	123:176	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
27959566	1	68	theme	nanocrystals	158:169	arg1	biocompatibility					101:116	biocompatibility	101:116	biocompatibility	101:116	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
27959566	1	68	theme	nanocrystals	158:169	arg1	renewability					87:98	The renewability	83:98	The renewability	83:98	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
27959566	1	69	theme	biomedical	240:249	arg1	applications					268:279	numerous composite, biomedical, and rheological applications	220:279	numerous composite, biomedical, and rheological applications	220:279	The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications.
25299624	6	0	theme	dendrimer	923:931	arg1	membranes					973:981	the dendrimer crosslinked chitosan-collagen composite membranes	919:981	the dendrimer crosslinked chitosan-collagen composite membranes	919:981	Overall, the dendrimer crosslinked chitosan-collagen composite membranes showed promising properties that suggest that these might be suitable biomaterials for corneal tissue-engineering applications.
25299624	4	1	theme	chitosan	719:726	arg1	sample					709:714	the sample	705:714	the sample of chitosan	705:726	As a result, the optical transparency and mechanical strength of the chitosan-collagen membranes were significantly better than that of the sample of chitosan.
25299624	4	2	theme	optical	586:592	arg1	transparency					594:605	optical transparency	586:605	optical transparency	586:605	As a result, the optical transparency and mechanical strength of the chitosan-collagen membranes were significantly better than that of the sample of chitosan.
25299624	5	3	contain	possess	879:885	arg1	collagen					790:797	the collagen	786:797	the collagen	786:797	In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
25299624	5	3	contain	possess	879:885	arg2	biocompatibility					892:907	good biocompatibility	887:907	good biocompatibility	887:907	In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
25299624	3	4	theme	chitosan	514:521	arg1	membrane					523:530	chitosan membrane	514:530	chitosan membrane	514:530	Mechanical properties, contact angles, and optical transmittance were determined and compared between chitosan membrane and crosslinking composite membrane.
25299624	5	5	theme	in	742:743	arg1	studies					764:770	in vitro cell culture studies	742:770	in vitro cell culture studies	742:770	In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
25299624	5	6	theme	cell	751:754	arg1	studies					764:770	in vitro cell culture studies	742:770	in vitro cell culture studies	742:770	In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
25299624	1	7	theme	chitosan-collagen	133:149	arg1	membrane					161:168	a chitosan-collagen composite membrane	131:168	a chitosan-collagen composite membrane as corneal tissue-engineering biomaterials	131:211	This article describes a chitosan-collagen composite membrane as corneal tissue-engineering biomaterials.
25299624	3	8	theme	crosslinking	536:547	arg1	membrane					559:566	crosslinking composite membrane	536:566	crosslinking composite membrane	536:566	Mechanical properties, contact angles, and optical transmittance were determined and compared between chitosan membrane and crosslinking composite membrane.
25299624	6	9	link	crosslinked	933:943	arg1	membranes					973:981	the dendrimer crosslinked chitosan-collagen composite membranes	919:981	the dendrimer crosslinked chitosan-collagen composite membranes	919:981	Overall, the dendrimer crosslinked chitosan-collagen composite membranes showed promising properties that suggest that these might be suitable biomaterials for corneal tissue-engineering applications.
25299624	2	10	theme	3-dimethylaminopropyl	375:395	arg1	carbodiimide					398:409	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	364:409	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	364:409	The membrane was prepared by dissolving the chitosan into collagen with the weight ratio of 0, 15, 30, 45, 60, and 100%, followed by crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.
25299624	5	11	theme	culture	756:762	arg1	studies					764:770	in vitro cell culture studies	742:770	in vitro cell culture studies	742:770	In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
25299624	3	12	theme	composite	549:557	arg1	membrane					559:566	crosslinking composite membrane	536:566	crosslinking composite membrane	536:566	Mechanical properties, contact angles, and optical transmittance were determined and compared between chitosan membrane and crosslinking composite membrane.
25299624	2	13	theme	1-ethyl-3-	364:373	arg1	carbodiimide					398:409	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	364:409	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	364:409	The membrane was prepared by dissolving the chitosan into collagen with the weight ratio of 0, 15, 30, 45, 60, and 100%, followed by crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.
25299624	0	14	link	crosslinked	54:64	arg1	membranes					66:74	chitosan-collagen crosslinked membranes	36:74	chitosan-collagen crosslinked membranes	36:74	Fabrication and characterization of chitosan-collagen crosslinked membranes for corneal tissue engineering.
25299624	4	15	theme	membranes	656:664	arg1	transparency					594:605	optical transparency	586:605	optical transparency	586:605	As a result, the optical transparency and mechanical strength of the chitosan-collagen membranes were significantly better than that of the sample of chitosan.
25299624	4	15	theme	membranes	656:664	arg1	strength					622:629	mechanical strength	611:629	mechanical strength	611:629	As a result, the optical transparency and mechanical strength of the chitosan-collagen membranes were significantly better than that of the sample of chitosan.
25299624	5	16	theme	cell	829:832	arg1	morphology					834:843	the cell morphology	825:843	the cell morphology	825:843	In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
25299624	4	17	theme	chitosan-collagen	638:654	arg1	membranes					656:664	the chitosan-collagen membranes	634:664	the chitosan-collagen membranes	634:664	As a result, the optical transparency and mechanical strength of the chitosan-collagen membranes were significantly better than that of the sample of chitosan.
25299624	0	18	theme	crosslinked	54:64	arg1	membranes					66:74	chitosan-collagen crosslinked membranes	36:74	chitosan-collagen crosslinked membranes	36:74	Fabrication and characterization of chitosan-collagen crosslinked membranes for corneal tissue engineering.
25299624	6	19	theme	promising	990:998	arg1	properties					1000:1009	promising properties	990:1009	promising properties that suggest that these might be suitable biomaterials for corneal tissue-engineering applications	990:1108	Overall, the dendrimer crosslinked chitosan-collagen composite membranes showed promising properties that suggest that these might be suitable biomaterials for corneal tissue-engineering applications.
25299624	4	20	dep	transparency	594:605	arg1	the					582:584	the	582:584	the	582:584	As a result, the optical transparency and mechanical strength of the chitosan-collagen membranes were significantly better than that of the sample of chitosan.
25299624	0	21	theme	chitosan-collagen	36:52	arg1	membranes					66:74	chitosan-collagen crosslinked membranes	36:74	chitosan-collagen crosslinked membranes	36:74	Fabrication and characterization of chitosan-collagen crosslinked membranes for corneal tissue engineering.
25299624	3	22	theme	Mechanical	412:421	arg1	properties					423:432	Mechanical properties	412:432	Mechanical properties	412:432	Mechanical properties, contact angles, and optical transmittance were determined and compared between chitosan membrane and crosslinking composite membrane.
25299624	6	23	theme	tissue-engineering	1078:1095	arg1	applications					1097:1108	corneal tissue-engineering applications	1070:1108	corneal tissue-engineering applications	1070:1108	Overall, the dendrimer crosslinked chitosan-collagen composite membranes showed promising properties that suggest that these might be suitable biomaterials for corneal tissue-engineering applications.
25299624	5	24	theme	good	887:890	arg1	biocompatibility					892:907	good biocompatibility	887:907	good biocompatibility	887:907	In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
25299624	5	25	theme	negative	806:813	arg1	effect					815:820	no negative effect	803:820	no negative effect	803:820	In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
25299624	3	26	theme	contact	435:441	arg1	angles					443:448	contact angles	435:448	contact angles	435:448	Mechanical properties, contact angles, and optical transmittance were determined and compared between chitosan membrane and crosslinking composite membrane.
25299624	2	27	theme	%	332:332	arg1	ratio					297:301	the weight ratio	286:301	the weight ratio of 0, 15, 30, 45, 60, and 100%	286:332	The membrane was prepared by dissolving the chitosan into collagen with the weight ratio of 0, 15, 30, 45, 60, and 100%, followed by crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.
25299624	0	28	theme	membranes	66:74	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of chitosan-collagen crosslinked membranes for corneal tissue engineering.
25299624	0	28	theme	membranes	66:74	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of chitosan-collagen crosslinked membranes for corneal tissue engineering.
25299624	6	29	theme	composite	963:971	arg1	membranes					973:981	the dendrimer crosslinked chitosan-collagen composite membranes	919:981	the dendrimer crosslinked chitosan-collagen composite membranes	919:981	Overall, the dendrimer crosslinked chitosan-collagen composite membranes showed promising properties that suggest that these might be suitable biomaterials for corneal tissue-engineering applications.
25299624	1	30	theme	composite	151:159	arg1	membrane					161:168	a chitosan-collagen composite membrane	131:168	a chitosan-collagen composite membrane as corneal tissue-engineering biomaterials	131:211	This article describes a chitosan-collagen composite membrane as corneal tissue-engineering biomaterials.
25299624	6	31	theme	chitosan-collagen	945:961	arg1	membranes					973:981	the dendrimer crosslinked chitosan-collagen composite membranes	919:981	the dendrimer crosslinked chitosan-collagen composite membranes	919:981	Overall, the dendrimer crosslinked chitosan-collagen composite membranes showed promising properties that suggest that these might be suitable biomaterials for corneal tissue-engineering applications.
25299624	5	32	dep	in	742:743	arg1	vitro					745:749	vitro	745:749	vitro	745:749	In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
25299624	6	33	theme	corneal	1070:1076	arg1	applications					1097:1108	corneal tissue-engineering applications	1070:1108	corneal tissue-engineering applications	1070:1108	Overall, the dendrimer crosslinked chitosan-collagen composite membranes showed promising properties that suggest that these might be suitable biomaterials for corneal tissue-engineering applications.
25299624	5	34	contain	has	799:801	arg1	collagen					790:797	the collagen	786:797	the collagen	786:797	In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
25299624	5	34	contain	has	799:801	arg2	effect					815:820	no negative effect	803:820	no negative effect	803:820	In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
25299624	4	35	theme	mechanical	611:620	arg1	strength					622:629	mechanical strength	611:629	mechanical strength	611:629	As a result, the optical transparency and mechanical strength of the chitosan-collagen membranes were significantly better than that of the sample of chitosan.
25299624	0	36	theme	tissue	88:93	arg1	engineering					95:105	corneal tissue engineering	80:105	corneal tissue engineering	80:105	Fabrication and characterization of chitosan-collagen crosslinked membranes for corneal tissue engineering.
25299624	3	37	theme	optical	455:461	arg1	transmittance					463:475	optical transmittance	455:475	optical transmittance	455:475	Mechanical properties, contact angles, and optical transmittance were determined and compared between chitosan membrane and crosslinking composite membrane.
25299624	1	38	theme	corneal	173:179	arg1	biomaterials					200:211	corneal tissue-engineering biomaterials	173:211	corneal tissue-engineering biomaterials	173:211	This article describes a chitosan-collagen composite membrane as corneal tissue-engineering biomaterials.
25299624	0	39	theme	corneal	80:86	arg1	engineering					95:105	corneal tissue engineering	80:105	corneal tissue engineering	80:105	Fabrication and characterization of chitosan-collagen crosslinked membranes for corneal tissue engineering.
25299624	6	40	theme	crosslinked	933:943	arg1	membranes					973:981	the dendrimer crosslinked chitosan-collagen composite membranes	919:981	the dendrimer crosslinked chitosan-collagen composite membranes	919:981	Overall, the dendrimer crosslinked chitosan-collagen composite membranes showed promising properties that suggest that these might be suitable biomaterials for corneal tissue-engineering applications.
25299624	2	41	theme	weight	290:295	arg1	ratio					297:301	the weight ratio	286:301	the weight ratio of 0, 15, 30, 45, 60, and 100%	286:332	The membrane was prepared by dissolving the chitosan into collagen with the weight ratio of 0, 15, 30, 45, 60, and 100%, followed by crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.
25299624	1	42	theme	tissue-engineering	181:198	arg1	biomaterials					200:211	corneal tissue-engineering biomaterials	173:211	corneal tissue-engineering biomaterials	173:211	This article describes a chitosan-collagen composite membrane as corneal tissue-engineering biomaterials.
25299624	6	43	theme	suitable	1044:1051	arg1	biomaterials					1053:1064	suitable biomaterials	1044:1064	suitable biomaterials for corneal tissue-engineering applications	1044:1108	Overall, the dendrimer crosslinked chitosan-collagen composite membranes showed promising properties that suggest that these might be suitable biomaterials for corneal tissue-engineering applications.
24321490	0	0	theme	phosphate	90:98	arg1	anions					100:105	phosphate anions	90:105	phosphate anions	90:105	A novel quaternized chitosan-melamine-glutaraldehyde resin for the removal of nitrate and phosphate anions.
24321490	1	1	theme	first	178:182	arg1	time					184:187	the first time	174:187	the first time	174:187	A terpolymer of chitosan-melamine-glutaraldehyde was prepared for the first time and it was quaternized with glycidyl trimethyl ammonium chloride (GTMAC).
24321490	7	2	theme	exchange	1016:1023	arg1	mechanism					1025:1033	exchange mechanism	1016:1033	exchange mechanism	1016:1033	Nitrate and phosphate anions adsorbed effectively on QCMGR by replacing Cl(-) ions at the quaternary ammonium group by exchange mechanism.
24321490	4	3	theme	Batch	495:499	arg1	method					501:506	Batch method	495:506	Batch method	495:506	Batch method was followed to optimise the conditions for the removal of nitrate and phosphate.
24321490	9	4	theme	QCMGR	1171:1175	arg1	Nitrate					1127:1133	Nitrate	1127:1133	Nitrate	1127:1133	Nitrate and phosphate removal efficiency of QCMGR was also tested by column method.
24321490	9	4	theme	QCMGR	1171:1175	arg1	efficiency					1157:1166	phosphate removal efficiency	1139:1166	phosphate removal efficiency of QCMGR	1139:1175	Nitrate and phosphate removal efficiency of QCMGR was also tested by column method.
24321490	5	5	theme	adsorption	703:712	arg1	process					714:720	the adsorption process	699:720	the adsorption process	699:720	Chemical kinetics of the adsorption was well fitted by pseudo-second order and particle diffusion models and the adsorption process followed the Freundlich isotherm model well.
24321490	9	6	theme	column	1196:1201	arg1	method					1203:1208	column method	1196:1208	column method	1196:1208	Nitrate and phosphate removal efficiency of QCMGR was also tested by column method.
24321490	7	7	theme	Cl	969:970	arg1	ions					975:978	Cl(-) ions	969:978	Cl(-) ions	969:978	Nitrate and phosphate anions adsorbed effectively on QCMGR by replacing Cl(-) ions at the quaternary ammonium group by exchange mechanism.
24321490	4	8	theme	nitrate	567:573	arg1	removal					556:562	the removal	552:562	the removal of nitrate and phosphate	552:587	Batch method was followed to optimise the conditions for the removal of nitrate and phosphate.
24321490	0	9	theme	anions	100:105	arg1	removal					67:73	the removal	63:73	the removal of nitrate and phosphate anions	63:105	A novel quaternized chitosan-melamine-glutaraldehyde resin for the removal of nitrate and phosphate anions.
24321490	6	10	theme	adsorption	793:802	arg1	capacity					804:811	phosphate adsorption capacity	783:811	phosphate adsorption capacity of QCMGR from 1000 mg/L	783:835	The nitrate and phosphate adsorption capacity of QCMGR from 1000 mg/L respective solutions were 97.5 and 112.5mg/g, respectively.
24321490	2	11	theme	nitrate	371:377	arg1	removal					360:366	the removal	356:366	the removal of nitrate and phosphate oxyanions	356:401	The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR) was found to be effective for the removal of nitrate and phosphate oxyanions.
24321490	5	12	theme	Chemical	590:597	arg1	kinetics					599:606	Chemical kinetics	590:606	Chemical kinetics of the adsorption	590:624	Chemical kinetics of the adsorption was well fitted by pseudo-second order and particle diffusion models and the adsorption process followed the Freundlich isotherm model well.
24321490	8	13	theme	10th	1047:1050	arg1	cycle					1065:1069	10th regeneration cycle	1047:1069	10th regeneration cycle	1047:1069	Even after 10th regeneration cycle the adsorbent fully retained its adsorption efficiency.
24321490	5	14	theme	Freundlich	735:744	arg1	model					755:759	the Freundlich isotherm model	731:759	the Freundlich isotherm model	731:759	Chemical kinetics of the adsorption was well fitted by pseudo-second order and particle diffusion models and the adsorption process followed the Freundlich isotherm model well.
24321490	6	15	dep	nitrate	771:777	arg1	solutions					848:856	respective solutions	837:856	respective solutions	837:856	The nitrate and phosphate adsorption capacity of QCMGR from 1000 mg/L respective solutions were 97.5 and 112.5mg/g, respectively.
24321490	3	16	dep	FTIR	437:440	arg1	regain					478:483	regain	478:483	regain property	478:492	The resin was characterised with FTIR, SEM, EDAX, TGA, DTA, XRD and water regain property.
24321490	2	17	theme	oxyanions	393:401	arg1	removal					360:366	the removal	356:366	the removal of nitrate and phosphate oxyanions	356:401	The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR) was found to be effective for the removal of nitrate and phosphate oxyanions.
24321490	0	18	theme	chitosan-melamine-glutaraldehyde	20:51	arg1	resin					53:57	chitosan-melamine-glutaraldehyde resin	20:57	chitosan-melamine-glutaraldehyde resin	20:57	A novel quaternized chitosan-melamine-glutaraldehyde resin for the removal of nitrate and phosphate anions.
24321490	5	19	theme	diffusion	678:686	arg1	models					688:693	particle diffusion models	669:693	particle diffusion models	669:693	Chemical kinetics of the adsorption was well fitted by pseudo-second order and particle diffusion models and the adsorption process followed the Freundlich isotherm model well.
24321490	6	20	theme	phosphate	783:791	arg1	capacity					804:811	phosphate adsorption capacity	783:811	phosphate adsorption capacity of QCMGR from 1000 mg/L	783:835	The nitrate and phosphate adsorption capacity of QCMGR from 1000 mg/L respective solutions were 97.5 and 112.5mg/g, respectively.
24321490	8	21	theme	regeneration	1052:1063	arg1	cycle					1065:1069	10th regeneration cycle	1047:1069	10th regeneration cycle	1047:1069	Even after 10th regeneration cycle the adsorbent fully retained its adsorption efficiency.
24321490	1	22	theme	glycidyl	217:224	arg1	GTMAC					255:259	GTMAC	255:259	GTMAC	255:259	A terpolymer of chitosan-melamine-glutaraldehyde was prepared for the first time and it was quaternized with glycidyl trimethyl ammonium chloride (GTMAC).
24321490	1	22	theme	glycidyl	217:224	arg1	chloride					245:252	glycidyl trimethyl ammonium chloride	217:252	glycidyl trimethyl ammonium chloride (GTMAC)	217:260	A terpolymer of chitosan-melamine-glutaraldehyde was prepared for the first time and it was quaternized with glycidyl trimethyl ammonium chloride (GTMAC).
24321490	6	23	from	capacity	804:811	arg1	mg/L					832:835	1000 mg/L	827:835	1000 mg/L	827:835	The nitrate and phosphate adsorption capacity of QCMGR from 1000 mg/L respective solutions were 97.5 and 112.5mg/g, respectively.
24321490	1	24	theme	trimethyl	226:234	arg1	GTMAC					255:259	GTMAC	255:259	GTMAC	255:259	A terpolymer of chitosan-melamine-glutaraldehyde was prepared for the first time and it was quaternized with glycidyl trimethyl ammonium chloride (GTMAC).
24321490	1	24	theme	trimethyl	226:234	arg1	chloride					245:252	glycidyl trimethyl ammonium chloride	217:252	glycidyl trimethyl ammonium chloride (GTMAC)	217:260	A terpolymer of chitosan-melamine-glutaraldehyde was prepared for the first time and it was quaternized with glycidyl trimethyl ammonium chloride (GTMAC).
24321490	7	25	theme	quaternary	987:996	arg1	group					1007:1011	the quaternary ammonium group	983:1011	the quaternary ammonium group by exchange mechanism	983:1033	Nitrate and phosphate anions adsorbed effectively on QCMGR by replacing Cl(-) ions at the quaternary ammonium group by exchange mechanism.
24321490	1	26	theme	ammonium	236:243	arg1	GTMAC					255:259	GTMAC	255:259	GTMAC	255:259	A terpolymer of chitosan-melamine-glutaraldehyde was prepared for the first time and it was quaternized with glycidyl trimethyl ammonium chloride (GTMAC).
24321490	1	26	theme	ammonium	236:243	arg1	chloride					245:252	glycidyl trimethyl ammonium chloride	217:252	glycidyl trimethyl ammonium chloride (GTMAC)	217:260	A terpolymer of chitosan-melamine-glutaraldehyde was prepared for the first time and it was quaternized with glycidyl trimethyl ammonium chloride (GTMAC).
24321490	6	27	from	mg/L	832:835	arg1	QCMGR					816:820	QCMGR	816:820	QCMGR from 1000 mg/L	816:835	The nitrate and phosphate adsorption capacity of QCMGR from 1000 mg/L respective solutions were 97.5 and 112.5mg/g, respectively.
24321490	6	27	from	mg/L	832:835	arg1	capacity					804:811	phosphate adsorption capacity	783:811	phosphate adsorption capacity of QCMGR from 1000 mg/L	783:835	The nitrate and phosphate adsorption capacity of QCMGR from 1000 mg/L respective solutions were 97.5 and 112.5mg/g, respectively.
24321490	5	28	theme	pseudo-second	645:657	arg1	order					659:663	pseudo-second order	645:663	pseudo-second order	645:663	Chemical kinetics of the adsorption was well fitted by pseudo-second order and particle diffusion models and the adsorption process followed the Freundlich isotherm model well.
24321490	6	29	theme	respective	837:846	arg1	solutions					848:856	respective solutions	837:856	respective solutions	837:856	The nitrate and phosphate adsorption capacity of QCMGR from 1000 mg/L respective solutions were 97.5 and 112.5mg/g, respectively.
24321490	7	30	theme	ammonium	998:1005	arg1	group					1007:1011	the quaternary ammonium group	983:1011	the quaternary ammonium group by exchange mechanism	983:1033	Nitrate and phosphate anions adsorbed effectively on QCMGR by replacing Cl(-) ions at the quaternary ammonium group by exchange mechanism.
24321490	1	31	theme	chitosan-melamine-glutaraldehyde	124:155	arg1	terpolymer					110:119	A terpolymer	108:119	A terpolymer of chitosan-melamine-glutaraldehyde	108:155	A terpolymer of chitosan-melamine-glutaraldehyde was prepared for the first time and it was quaternized with glycidyl trimethyl ammonium chloride (GTMAC).
24321490	8	32	theme	adsorption	1104:1113	arg1	efficiency					1115:1124	its adsorption efficiency	1100:1124	its adsorption efficiency	1100:1124	Even after 10th regeneration cycle the adsorbent fully retained its adsorption efficiency.
24321490	2	33	theme	chitosan-melamine-glutaraldehyde	279:310	arg1	QCMGR					319:323	QCMGR	319:323	QCMGR	319:323	The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR) was found to be effective for the removal of nitrate and phosphate oxyanions.
24321490	2	33	theme	chitosan-melamine-glutaraldehyde	279:310	arg1	resin					312:316	The quaternized chitosan-melamine-glutaraldehyde resin	263:316	The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR)	263:324	The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR) was found to be effective for the removal of nitrate and phosphate oxyanions.
24321490	2	33	theme	chitosan-melamine-glutaraldehyde	279:310	arg1	effective					342:350	effective	342:350	effective	342:350	The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR) was found to be effective for the removal of nitrate and phosphate oxyanions.
24321490	7	34	dep	Nitrate	897:903	arg1	anions					919:924	anions	919:924	anions	919:924	Nitrate and phosphate anions adsorbed effectively on QCMGR by replacing Cl(-) ions at the quaternary ammonium group by exchange mechanism.
24321490	4	35	theme	phosphate	579:587	arg1	removal					556:562	the removal	552:562	the removal of nitrate and phosphate	552:587	Batch method was followed to optimise the conditions for the removal of nitrate and phosphate.
24321490	5	36	theme	particle	669:676	arg1	models					688:693	particle diffusion models	669:693	particle diffusion models	669:693	Chemical kinetics of the adsorption was well fitted by pseudo-second order and particle diffusion models and the adsorption process followed the Freundlich isotherm model well.
24321490	2	37	theme	quaternized	267:277	arg1	QCMGR					319:323	QCMGR	319:323	QCMGR	319:323	The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR) was found to be effective for the removal of nitrate and phosphate oxyanions.
24321490	2	37	theme	quaternized	267:277	arg1	resin					312:316	The quaternized chitosan-melamine-glutaraldehyde resin	263:316	The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR)	263:324	The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR) was found to be effective for the removal of nitrate and phosphate oxyanions.
24321490	2	37	theme	quaternized	267:277	arg1	effective					342:350	effective	342:350	effective	342:350	The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR) was found to be effective for the removal of nitrate and phosphate oxyanions.
24321490	9	38	theme	phosphate	1139:1147	arg1	efficiency					1157:1166	phosphate removal efficiency	1139:1166	phosphate removal efficiency of QCMGR	1139:1175	Nitrate and phosphate removal efficiency of QCMGR was also tested by column method.
24321490	0	39	theme	nitrate	78:84	arg1	removal					67:73	the removal	63:73	the removal of nitrate and phosphate anions	63:105	A novel quaternized chitosan-melamine-glutaraldehyde resin for the removal of nitrate and phosphate anions.
24321490	6	40	theme	QCMGR	816:820	arg1	capacity					804:811	phosphate adsorption capacity	783:811	phosphate adsorption capacity of QCMGR from 1000 mg/L	783:835	The nitrate and phosphate adsorption capacity of QCMGR from 1000 mg/L respective solutions were 97.5 and 112.5mg/g, respectively.
24321490	9	41	theme	removal	1149:1155	arg1	efficiency					1157:1166	phosphate removal efficiency	1139:1166	phosphate removal efficiency of QCMGR	1139:1175	Nitrate and phosphate removal efficiency of QCMGR was also tested by column method.
24321490	2	42	theme	phosphate	383:391	arg1	oxyanions					393:401	phosphate oxyanions	383:401	phosphate oxyanions	383:401	The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR) was found to be effective for the removal of nitrate and phosphate oxyanions.
24321490	5	43	theme	isotherm	746:753	arg1	model					755:759	the Freundlich isotherm model	731:759	the Freundlich isotherm model	731:759	Chemical kinetics of the adsorption was well fitted by pseudo-second order and particle diffusion models and the adsorption process followed the Freundlich isotherm model well.
24321490	5	44	theme	adsorption	615:624	arg1	kinetics					599:606	Chemical kinetics	590:606	Chemical kinetics of the adsorption	590:624	Chemical kinetics of the adsorption was well fitted by pseudo-second order and particle diffusion models and the adsorption process followed the Freundlich isotherm model well.
25763541	7	0	theme	mono-glycidyl	2114:2126	arg1	spacers					2149:2155	mono-glycidyl methacrylate linking spacers	2114:2155	mono-glycidyl methacrylate linking spacers	2114:2155	It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
25763541	3	1	theme	polymerization	778:791	arg1	conditions					793:802	The polymerization conditions	774:802	The polymerization conditions for both monoliths	774:821	The polymerization conditions for both monoliths were carefully optimized to obtain satisfactory column performance with respect to column efficiency, reproducibility, permeability, and stability.
25763541	0	2	theme	column	177:182	arg1	performance					184:194	the column performance	173:194	the column performance	173:194	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	1	3	theme	strategy	266:273	arg1	applicability					237:249	applicability	237:249	applicability	237:249	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	1	3	theme	strategy	266:273	arg1	feasibility					221:231	feasibility	221:231	feasibility	221:231	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	1	4	from	feasibility	221:231	arg1	preparation					296:306	monolithic column preparation	278:306	monolithic column preparation	278:306	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	1	5	theme	monolithic	278:287	arg1	preparation					296:306	monolithic column preparation	278:306	monolithic column preparation	278:306	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	4	6	theme	micro-high-performance	1310:1331	arg1	chromatography					1340:1353	micro-high-performance liquid chromatography	1310:1353	micro-high-performance liquid chromatography	1310:1353	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	5	7	theme	multi-glycidyl	1414:1427	arg1	methacrylate					1429:1440	multi-glycidyl methacrylate	1414:1440	multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith	1414:1568	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	2	8	theme	functional	688:697	arg1	monomers					699:706	the corresponding functional monomers	670:706	the corresponding functional monomers	670:706	In this improved method, mono(6-amino-6-deoxy)-β-cyclodextrin or heptakis(6-amino-6-deoxy)-β-cyclodextrin reacted with glycidyl methacrylate to generate the corresponding functional monomers and were subsequently copolymerized with ethylene dimethacrylate.
25763541	3	9	theme	satisfactory	858:869	arg1	performance					878:888	satisfactory column performance	858:888	satisfactory column performance	858:888	The polymerization conditions for both monoliths were carefully optimized to obtain satisfactory column performance with respect to column efficiency, reproducibility, permeability, and stability.
25763541	1	10	from	preparation	296:306	arg1	applicability					237:249	applicability	237:249	applicability	237:249	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	1	10	from	preparation	296:306	arg1	feasibility					221:231	feasibility	221:231	feasibility	221:231	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	4	11	theme	scanning	1277:1284	arg1	microscopy					1295:1304	scanning electron microscopy	1277:1304	scanning electron microscopy	1277:1304	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	0	12	theme	degree	123:128	arg1	Effect					109:114	Effect	109:114	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.	0:195	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	4	13	theme	poly	1080:1083	arg1	monoliths					1176:1184	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths	971:1184	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths	971:1184	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	5	14	theme	methacrylate	1429:1440	arg1	existence					1401:1409	the probable existence	1388:1409	the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith	1388:1568	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	2	15	theme	glycidyl	636:643	arg1	methacrylate					645:656	glycidyl methacrylate	636:656	glycidyl methacrylate	636:656	In this improved method, mono(6-amino-6-deoxy)-β-cyclodextrin or heptakis(6-amino-6-deoxy)-β-cyclodextrin reacted with glycidyl methacrylate to generate the corresponding functional monomers and were subsequently copolymerized with ethylene dimethacrylate.
25763541	4	16	theme	-β-cyclodextrin-co-ethylene	1032:1058	arg1	poly					984:987	poly	984:987	poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate)	984:1074	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	4	16	theme	-β-cyclodextrin-co-ethylene	1032:1058	arg1	dimethacrylate					1060:1073	glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate	989:1073	glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate	989:1073	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	5	17	theme	-β-cyclodextrin-co-ethylene	1517:1543	arg1	monolith					1561:1568	the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith	1461:1568	the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith	1461:1568	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	1	18	from	applicability	237:249	arg1	preparation					296:306	monolithic column preparation	278:306	monolithic column preparation	278:306	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	6	19	theme	amino	1856:1860	arg1	substitution					1862:1873	amino substitution	1856:1873	amino substitution of β-cyclodextrin	1856:1891	To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids.
25763541	3	20	theme	column	906:911	arg1	efficiency					913:922	column efficiency	906:922	column efficiency	906:922	The polymerization conditions for both monoliths were carefully optimized to obtain satisfactory column performance with respect to column efficiency, reproducibility, permeability, and stability.
25763541	6	21	theme	degree	1846:1851	arg1	effect					1832:1837	the effect	1828:1837	the effect of the degree of amino substitution of β-cyclodextrin on column performance	1828:1913	To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids.
25763541	6	22	theme	chiral	2034:2039	arg1	acids					2041:2045	chiral acids	2034:2045	chiral acids	2034:2045	To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids.
25763541	0	23	theme	β-cyclodextrin-functionalized	68:96	arg1	monoliths					98:106	β-cyclodextrin-functionalized monoliths	68:106	β-cyclodextrin-functionalized monoliths	68:106	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	6	24	theme	monoliths	1949:1957	arg1	comparison					1927:1936	a detailed comparison	1916:1936	a detailed comparison of the two monoliths	1916:1957	To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids.
25763541	6	25	from	effect	1832:1837	arg1	performance					1903:1913	column performance	1896:1913	column performance	1896:1913	To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids.
25763541	0	26	theme	Comparative	0:10	arg1	evaluation					12:21	Comparative evaluation	0:21	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.	0:195	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	1	27	theme	column	289:294	arg1	preparation					296:306	monolithic column preparation	278:306	monolithic column preparation	278:306	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	4	28	theme	good	1217:1220	arg1	permeability					1222:1233	good permeability	1217:1233	good permeability	1217:1233	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	2	29	theme	ethylene	749:756	arg1	dimethacrylate					758:771	ethylene dimethacrylate	749:771	ethylene dimethacrylate	749:771	In this improved method, mono(6-amino-6-deoxy)-β-cyclodextrin or heptakis(6-amino-6-deoxy)-β-cyclodextrin reacted with glycidyl methacrylate to generate the corresponding functional monomers and were subsequently copolymerized with ethylene dimethacrylate.
25763541	7	30	theme	linking	2250:2256	arg1	spacers					2258:2264	multi-glycidyl methacrylate linking spacers	2222:2264	multi-glycidyl methacrylate linking spacers	2222:2264	It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
25763541	2	31	theme	improved	525:532	arg1	method					534:539	this improved method	520:539	this improved method	520:539	In this improved method, mono(6-amino-6-deoxy)-β-cyclodextrin or heptakis(6-amino-6-deoxy)-β-cyclodextrin reacted with glycidyl methacrylate to generate the corresponding functional monomers and were subsequently copolymerized with ethylene dimethacrylate.
25763541	0	32	theme	one-pot	28:34	arg1	strategy					36:43	a one-pot strategy	26:43	a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths	26:106	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	1	33	theme	novel	313:317	arg1	monoliths					367:375	two novel β-cyclodextrin-functionalized organic polymeric monoliths	309:375	two novel β-cyclodextrin-functionalized organic polymeric monoliths	309:375	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	7	34	theme	multi-glycidyl	2222:2235	arg1	spacers					2258:2264	multi-glycidyl methacrylate linking spacers	2222:2264	multi-glycidyl methacrylate linking spacers	2222:2264	It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
25763541	7	35	theme	better	2170:2175	arg1	performance					2195:2205	better chiral separation performance	2170:2205	better chiral separation performance	2170:2205	It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
25763541	5	36	theme	polymerization	1789:1802	arg1	mixture					1804:1810	the polymerization mixture	1785:1810	the polymerization mixture	1785:1810	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	5	37	theme	-β-cyclodextrin	1645:1659	arg1	ratio					1589:1593	the ratio	1585:1593	the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin	1585:1659	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	1	38	theme	organic	349:355	arg1	monoliths					367:375	two novel β-cyclodextrin-functionalized organic polymeric monoliths	309:375	two novel β-cyclodextrin-functionalized organic polymeric monoliths	309:375	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	7	39	with	monolith	2100:2107	arg1	spacers					2149:2155	mono-glycidyl methacrylate linking spacers	2114:2155	mono-glycidyl methacrylate linking spacers	2114:2155	It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
25763541	7	40	theme	methacrylate	2128:2139	arg1	spacers					2149:2155	mono-glycidyl methacrylate linking spacers	2114:2155	mono-glycidyl methacrylate linking spacers	2114:2155	It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
25763541	5	41	theme	methacrylate/heptakis	1607:1627	arg1	-β-cyclodextrin					1645:1659	glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin	1598:1659	glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin	1598:1659	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	5	42	theme	6-amino-6-deoxy	1629:1643	arg1	-β-cyclodextrin					1645:1659	glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin	1598:1659	glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin	1598:1659	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	1	43	theme	one-pot	258:264	arg1	strategy					266:273	the one-pot strategy	254:273	the one-pot strategy in monolithic column preparation	254:306	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	4	44	theme	poly	984:987	arg1	monoliths					1176:1184	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths	971:1184	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths	971:1184	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	7	45	theme	β-cyclodextrin-functionalized	2070:2098	arg1	monolith					2100:2107	the β-cyclodextrin-functionalized monolith	2066:2107	the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers	2066:2155	It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
25763541	4	46	theme	mechanical	1240:1249	arg1	stability					1251:1259	mechanical stability	1240:1259	mechanical stability	1240:1259	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	1	47	theme	6-amino-6-deoxy	484:498	arg1	-β-cyclodextrin					500:514	heptakis(6-amino-6-deoxy)-β-cyclodextrin	475:514	heptakis(6-amino-6-deoxy)-β-cyclodextrin	475:514	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	4	48	theme	liquid	1333:1338	arg1	chromatography					1340:1353	micro-high-performance liquid chromatography	1310:1353	micro-high-performance liquid chromatography	1310:1353	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	1	49	dep	-β-cyclodextrin	455:469	arg1	i.e.					429:432	i.e.	429:432	i.e.	429:432	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	4	50	theme	experimental	1355:1366	arg1	results					1368:1374	experimental results	1355:1374	experimental results	1355:1374	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	1	51	from	strategy	266:273	arg1	preparation					296:306	monolithic column preparation	278:306	monolithic column preparation	278:306	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	2	52	theme	corresponding	674:686	arg1	monomers					699:706	the corresponding functional monomers	670:706	the corresponding functional monomers	670:706	In this improved method, mono(6-amino-6-deoxy)-β-cyclodextrin or heptakis(6-amino-6-deoxy)-β-cyclodextrin reacted with glycidyl methacrylate to generate the corresponding functional monomers and were subsequently copolymerized with ethylene dimethacrylate.
25763541	4	53	theme	electron	1286:1293	arg1	microscopy					1295:1304	scanning electron microscopy	1277:1304	scanning electron microscopy	1277:1304	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	3	54	theme	column	871:876	arg1	performance					878:888	satisfactory column performance	858:888	satisfactory column performance	858:888	The polymerization conditions for both monoliths were carefully optimized to obtain satisfactory column performance with respect to column efficiency, reproducibility, permeability, and stability.
25763541	5	55	theme	satisfactory	1689:1700	arg1	reproducibility					1702:1716	satisfactory reproducibility	1689:1716	satisfactory reproducibility	1689:1716	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	4	56	theme	obtained	975:982	arg1	monoliths					1176:1184	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths	971:1184	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths	971:1184	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	0	57	theme	substitution	139:150	arg1	degree					123:128	the degree	119:128	the degree of amino substitution of β-cyclodextrin	119:168	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	1	58	theme	mono	434:437	arg1	-β-cyclodextrin					455:469	mono(6-amino-6-deoxy)-β-cyclodextrin	434:469	mono(6-amino-6-deoxy)-β-cyclodextrin	434:469	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	0	59	theme	β-cyclodextrin	155:168	arg1	substitution					139:150	amino substitution	133:150	amino substitution of β-cyclodextrin	133:168	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	1	60	theme	6-amino-6-deoxy	439:453	arg1	-β-cyclodextrin					455:469	mono(6-amino-6-deoxy)-β-cyclodextrin	434:469	mono(6-amino-6-deoxy)-β-cyclodextrin	434:469	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	4	61	dep	microscopy	1295:1304	arg1	results					1368:1374	experimental results	1355:1374	experimental results	1355:1374	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	6	62	theme	substitution	1862:1873	arg1	degree					1846:1851	the degree	1842:1851	the degree of amino substitution of β-cyclodextrin	1842:1891	To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids.
25763541	0	63	from	Effect	109:114	arg1	performance					184:194	the column performance	173:194	the column performance	173:194	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	5	64	theme	dimethacrylate	1545:1558	arg1	monolith					1561:1568	the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith	1461:1568	the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith	1461:1568	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	1	65	theme	heptakis	475:482	arg1	-β-cyclodextrin					500:514	heptakis(6-amino-6-deoxy)-β-cyclodextrin	475:514	heptakis(6-amino-6-deoxy)-β-cyclodextrin	475:514	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	6	66	theme	β-cyclodextrin	1878:1891	arg1	substitution					1862:1873	amino substitution	1856:1873	amino substitution of β-cyclodextrin	1856:1891	To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids.
25763541	0	67	theme	monoliths	98:106	arg1	preparation					53:63	the preparation	49:63	the preparation of β-cyclodextrin-functionalized monoliths	49:106	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	4	68	theme	-β-cyclodextrin-co-ethylene	1132:1158	arg1	poly					1080:1083	poly	1080:1083	poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate)	1080:1174	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	4	68	theme	-β-cyclodextrin-co-ethylene	1132:1158	arg1	dimethacrylate					1160:1173	glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate	1085:1173	glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate	1085:1173	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	6	69	theme	small	2015:2019	arg1	peptides					2021:2028	small peptides	2015:2028	small peptides	2015:2028	To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids.
25763541	1	70	theme	β-cyclodextrin	401:414	arg1	derivatives					416:426	two β-cyclodextrin derivatives	397:426	two β-cyclodextrin derivatives	397:426	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	6	71	theme	detailed	1918:1925	arg1	comparison					1927:1936	a detailed comparison	1916:1936	a detailed comparison of the two monoliths	1916:1957	To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids.
25763541	6	72	theme	analytes	1996:2003	arg1	series					1986:1991	series	1986:1991	series of analytes including small peptides and chiral acids	1986:2045	To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids.
25763541	5	73	theme	ratio	1589:1593	arg1	effect					1575:1580	the effect	1571:1580	the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin	1571:1659	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	4	74	theme	uniform	1198:1204	arg1	structure					1206:1214	a uniform structure	1196:1214	a uniform structure	1196:1214	The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results.
25763541	5	75	from	spacers	1450:1456	arg1	monolith					1561:1568	the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith	1461:1568	the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith	1461:1568	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	5	76	theme	probable	1392:1399	arg1	existence					1401:1409	the probable existence	1388:1409	the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith	1388:1568	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	7	77	theme	methacrylate	2237:2248	arg1	spacers					2258:2264	multi-glycidyl methacrylate linking spacers	2222:2264	multi-glycidyl methacrylate linking spacers	2222:2264	It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
25763541	6	78	theme	column	1896:1901	arg1	performance					1903:1913	column performance	1896:1913	column performance	1896:1913	To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids.
25763541	0	79	theme	strategy	36:43	arg1	evaluation					12:21	Comparative evaluation	0:21	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.	0:195	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	1	80	theme	β-cyclodextrin-functionalized	319:347	arg1	monoliths					367:375	two novel β-cyclodextrin-functionalized organic polymeric monoliths	309:375	two novel β-cyclodextrin-functionalized organic polymeric monoliths	309:375	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	7	81	theme	chiral	2177:2182	arg1	performance					2195:2205	better chiral separation performance	2170:2205	better chiral separation performance	2170:2205	It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
25763541	0	82	theme	amino	133:137	arg1	substitution					139:150	amino substitution	133:150	amino substitution of β-cyclodextrin	133:168	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	5	83	theme	mixture	1804:1810	arg1	composition					1770:1780	the composition	1766:1780	the composition of the polymerization mixture	1766:1810	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	1	84	theme	polymeric	357:365	arg1	monoliths					367:375	two novel β-cyclodextrin-functionalized organic polymeric monoliths	309:375	two novel β-cyclodextrin-functionalized organic polymeric monoliths	309:375	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	7	85	theme	separation	2184:2193	arg1	performance					2195:2205	better chiral separation performance	2170:2205	better chiral separation performance	2170:2205	It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
25763541	1	86	dep	feasibility	221:231	arg1	the					217:219	the	217:219	the	217:219	To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin.
25763541	5	87	theme	glycidyl	1598:1605	arg1	-β-cyclodextrin					1645:1659	glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin	1598:1659	glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin	1598:1659	Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin-co-ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture.
25763541	0	88	dep	evaluation	12:21	arg1	Effect					109:114	Effect	109:114	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.	0:195	Comparative evaluation of a one-pot strategy for the preparation of β-cyclodextrin-functionalized monoliths: Effect of the degree of amino substitution of β-cyclodextrin on the column performance.
25763541	7	89	theme	linking	2141:2147	arg1	spacers					2149:2155	mono-glycidyl methacrylate linking spacers	2114:2155	mono-glycidyl methacrylate linking spacers	2114:2155	It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
29050468	3	0	theme	Fluid	856:860	arg1	PC-SAFT					870:876	PC-SAFT	870:876	PC-SAFT	870:876	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	3	0	theme	Fluid	856:860	arg1	Theory					862:867	the Perturbed-Chain Statistical Associating Fluid Theory	812:867	the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT)	812:877	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	6	1	theme	RH	1264:1265	arg1	impact					1254:1259	The impact	1250:1259	The impact of RH on the stability	1250:1282	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	1	2	theme	hydroxypropyl	386:398	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	2	theme	hydroxypropyl	386:398	arg1	126G					434:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	6	3	theme	<	1354:1354	arg1	PVP					1366:1368	HPMCAS < PVPVA64 < PVP	1347:1368	HPMCAS < PVPVA64 < PVP	1347:1368	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	6	3	theme	<	1354:1354	arg1	polymers					1337:1344	the pure polymers	1328:1344	the pure polymers (HPMCAS < PVPVA64 < PVP)	1328:1369	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	5	4	theme	NAP	1172:1174	arg1	solubility					1176:1185	NAP solubility	1172:1185	the kinetic stabilization as well as NAP solubility	1135:1185	RH significantly reduces the kinetic stabilization as well as NAP solubility in the polymers, while the impact on APAP solubility is small.
29050468	7	5	theme	long-term	1606:1614	arg1	stability					1625:1633	long-term physical stability	1606:1633	long-term physical stability	1606:1633	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	2	6	theme	pharmaceutical	614:627	arg1	naproxen					579:586	naproxen	579:586	naproxen (NAP)	579:592	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	2	6	theme	pharmaceutical	614:627	arg1	APIs					642:645	APIs	642:645	APIs	642:645	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	2	6	theme	pharmaceutical	614:627	arg1	ingredients					629:639	active pharmaceutical ingredients	607:639	active pharmaceutical ingredients (APIs)	607:646	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	2	6	theme	pharmaceutical	614:627	arg1	Acetaminophen					554:566	Acetaminophen	554:566	Acetaminophen (APAP)	554:573	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	1	7	theme	acetate	416:422	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	7	theme	acetate	416:422	arg1	126G					434:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	4	8	from	APIs	980:983	arg1	polymers					992:999	the polymers	988:999	the polymers	988:999	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	4	9	theme	APIs	980:983	arg1	solubility					962:971	the solubility	958:971	the solubility of the APIs in the polymers	958:999	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	4	9	theme	APIs	980:983	arg1	ability					1029:1035	the kinetic stabilizing ability	1005:1035	the kinetic stabilizing ability of the polymers	1005:1051	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	1	10	theme	°C/75	543:547	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	10	theme	°C/75	543:547	arg1	RH					550:551	40 °C/75% RH	540:551	40 °C/75% RH	540:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	6	11	theme	pure	1332:1335	arg1	PVP					1366:1368	HPMCAS < PVPVA64 < PVP	1347:1368	HPMCAS < PVPVA64 < PVP	1347:1368	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	6	11	theme	pure	1332:1335	arg1	polymers					1337:1344	the pure polymers	1328:1344	the pure polymers (HPMCAS < PVPVA64 < PVP)	1328:1369	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	1	12	theme	physical	166:173	arg1	stability					175:183	the long-term physical stability	152:183	the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH	152:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	7	13	theme	storage	1577:1583	arg1	conditions					1585:1594	storage conditions	1577:1594	storage conditions	1577:1594	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	1	14	theme	vinylpyrrolidone-co-vinyl	336:360	arg1	poly					331:334	poly	331:334	poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64)	331:379	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	14	theme	vinylpyrrolidone-co-vinyl	336:360	arg1	acetate					362:368	vinylpyrrolidone-co-vinyl acetate	336:368	vinylpyrrolidone-co-vinyl acetate	336:368	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	15	theme	solid	198:202	arg1	formulations					221:232	amorphous solid dispersion (ASD) formulations	188:232	amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH	188:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	4	16	theme	kinetic	1009:1015	arg1	ability					1029:1035	the kinetic stabilizing ability	1005:1035	the kinetic stabilizing ability of the polymers	1005:1051	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	7	17	theme	ASD	1556:1558	arg1	compositions					1560:1571	appropriate ASD compositions	1544:1571	appropriate ASD compositions	1544:1571	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	3	18	theme	long	658:661	arg1	studies					673:679	18 month long stability studies	649:679	18 month long stability studies of these formulations	649:701	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	7	19	theme	stability	1389:1397	arg1	results					1399:1405	The experimental stability results	1372:1405	The experimental stability results	1372:1405	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	1	20	theme	°C/60	525:529	arg1	RH					532:533	25 °C/60% RH	522:533	25 °C/60% RH	522:533	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	20	theme	°C/60	525:529	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	3	21	theme	18	649:650	arg1	month					652:656	month	652:656	month	652:656	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	6	22	theme	<	1364:1364	arg1	PVP					1366:1368	HPMCAS < PVPVA64 < PVP	1347:1368	HPMCAS < PVPVA64 < PVP	1347:1368	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	6	22	theme	<	1364:1364	arg1	polymers					1337:1344	the pure polymers	1328:1344	the pure polymers (HPMCAS < PVPVA64 < PVP)	1328:1369	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	1	23	theme	work	133:136	arg1	purpose					117:123	The purpose	113:123	The purpose of this work	113:136	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	3	24	theme	API/polymer	739:749	arg1	diagrams					757:764	the API/polymer phase diagrams	735:764	the API/polymer phase diagrams	735:764	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	0	25	theme	Physical	62:69	arg1	Stability					71:79	the Long-Term Physical Stability	48:79	the Long-Term Physical Stability of Amorphous Solid Dispersions	48:110	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.
29050468	4	26	dep	showed	929:934	arg1	PVP					1105:1107	PVP	1105:1107	PVP	1105:1107	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	7	27	theme	suitable	1502:1509	arg1	tools					1522:1526	suitable predictive tools	1502:1526	suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability	1502:1633	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	7	27	theme	suitable	1502:1509	arg1	PC-SAFT					1468:1474	PC-SAFT	1468:1474	PC-SAFT	1468:1474	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	7	27	theme	suitable	1502:1509	arg1	equation					1489:1496	the Kwei equation	1480:1496	the Kwei equation	1480:1496	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	0	28	from	Impact	0:5	arg1	Stability					71:79	the Long-Term Physical Stability	48:79	the Long-Term Physical Stability of Amorphous Solid Dispersions	48:110	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.
29050468	0	29	theme	Solid	94:98	arg1	Dispersions					100:110	Amorphous Solid Dispersions	84:110	Amorphous Solid Dispersions	84:110	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.
29050468	1	30	from	conditions	477:486	arg1	acetate					362:368	vinylpyrrolidone-co-vinyl acetate	336:368	vinylpyrrolidone-co-vinyl acetate	336:368	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	30	from	conditions	477:486	arg1	PVP					325:327	PVP	325:327	PVP	325:327	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	30	from	conditions	477:486	arg1	HPMCAS					440:445	HPMCAS	440:445	HPMCAS	440:445	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	30	from	conditions	477:486	arg1	RH					550:551	40 °C/75% RH	540:551	40 °C/75% RH	540:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	30	from	conditions	477:486	arg1	RH					517:518	RH	517:518	RH	517:518	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	30	from	conditions	477:486	arg1	RH					532:533	25 °C/60% RH	522:533	25 °C/60% RH	522:533	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	30	from	conditions	477:486	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	30	from	conditions	477:486	arg1	PVPVA64					372:378	PVPVA64	372:378	PVPVA64	372:378	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	30	from	conditions	477:486	arg1	humidity					507:514	25 °C/0% relative humidity	489:514	25 °C/0% relative humidity	489:514	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	30	from	conditions	477:486	arg1	poly					331:334	poly	331:334	poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64)	331:379	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	30	from	conditions	477:486	arg1	K25					320:322	poly(vinylpyrrolidone) K25	297:322	poly(vinylpyrrolidone) K25 (PVP)	297:328	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	30	from	conditions	477:486	arg1	126G					434:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	31	theme	%	496:496	arg1	humidity					507:514	25 °C/0% relative humidity	489:514	25 °C/0% relative humidity	489:514	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	31	theme	%	496:496	arg1	K25					320:322	poly(vinylpyrrolidone) K25	297:322	poly(vinylpyrrolidone) K25 (PVP)	297:328	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	32	theme	different	249:257	arg1	RH					550:551	40 °C/75% RH	540:551	40 °C/75% RH	540:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	32	theme	different	249:257	arg1	RH					532:533	25 °C/60% RH	522:533	25 °C/60% RH	522:533	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	32	theme	different	249:257	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	32	theme	different	249:257	arg1	poly					331:334	poly	331:334	poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64)	331:379	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	32	theme	different	249:257	arg1	K25					320:322	poly(vinylpyrrolidone) K25	297:322	poly(vinylpyrrolidone) K25 (PVP)	297:328	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	32	theme	different	249:257	arg1	126G					434:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	0	33	theme	Polymer	10:16	arg1	Type					18:21	Polymer Type	10:21	Polymer Type	10:21	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.
29050468	1	34	theme	used	272:275	arg1	RH					550:551	40 °C/75% RH	540:551	40 °C/75% RH	540:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	34	theme	used	272:275	arg1	RH					532:533	25 °C/60% RH	522:533	25 °C/60% RH	522:533	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	34	theme	used	272:275	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	34	theme	used	272:275	arg1	poly					331:334	poly	331:334	poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64)	331:379	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	34	theme	used	272:275	arg1	K25					320:322	poly(vinylpyrrolidone) K25	297:322	poly(vinylpyrrolidone) K25 (PVP)	297:328	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	34	theme	used	272:275	arg1	126G					434:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	5	35	from	impact	1214:1219	arg1	solubility					1229:1238	APAP solubility	1224:1238	APAP solubility	1224:1238	RH significantly reduces the kinetic stabilization as well as NAP solubility in the polymers, while the impact on APAP solubility is small.
29050468	4	36	from	ability	1029:1035	arg1	polymers					992:999	the polymers	988:999	the polymers	988:999	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	3	37	theme	Perturbed-Chain	816:830	arg1	PC-SAFT					870:876	PC-SAFT	870:876	PC-SAFT	870:876	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	3	37	theme	Perturbed-Chain	816:830	arg1	Theory					862:867	the Perturbed-Chain Statistical Associating Fluid Theory	812:867	the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT)	812:877	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	0	38	theme	Humidity	36:43	arg1	Impact					0:5	Impact	0:5	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.	0:111	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.
29050468	1	39	theme	dispersion	204:213	arg1	formulations					221:232	amorphous solid dispersion (ASD) formulations	188:232	amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH	188:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	4	40	theme	dry	945:947	arg1	storage					949:955	dry storage	945:955	dry storage	945:955	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	1	41	theme	ICH	465:467	arg1	conditions					477:486	standardized ICH storage conditions	452:486	standardized ICH storage conditions	452:486	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	42	theme	ASD	216:218	arg1	formulations					221:232	amorphous solid dispersion (ASD) formulations	188:232	amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH	188:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	3	43	theme	Associating	844:854	arg1	PC-SAFT					870:876	PC-SAFT	870:876	PC-SAFT	870:876	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	3	43	theme	Associating	844:854	arg1	Theory					862:867	the Perturbed-Chain Statistical Associating Fluid Theory	812:867	the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT)	812:877	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	1	44	theme	formulations	221:232	arg1	stability					175:183	the long-term physical stability	152:183	the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH	152:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	7	45	theme	physical	1616:1623	arg1	stability					1625:1633	long-term physical stability	1606:1633	long-term physical stability	1606:1633	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	5	46	theme	APAP	1224:1227	arg1	solubility					1229:1238	APAP solubility	1224:1238	APAP solubility	1224:1238	RH significantly reduces the kinetic stabilization as well as NAP solubility in the polymers, while the impact on APAP solubility is small.
29050468	1	47	theme	methylcellulose	400:414	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	47	theme	methylcellulose	400:414	arg1	126G					434:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	6	48	theme	HPMCAS	1347:1352	arg1	PVP					1366:1368	HPMCAS < PVPVA64 < PVP	1347:1368	HPMCAS < PVPVA64 < PVP	1347:1368	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	6	48	theme	HPMCAS	1347:1352	arg1	polymers					1337:1344	the pure polymers	1328:1344	the pure polymers (HPMCAS < PVPVA64 < PVP)	1328:1369	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	3	49	theme	Kwei	904:907	arg1	equation					909:916	the Gordon-Taylor or Kwei equation	883:916	equation	909:916	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	2	50	theme	active	607:612	arg1	naproxen					579:586	naproxen	579:586	naproxen (NAP)	579:592	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	2	50	theme	active	607:612	arg1	APIs					642:645	APIs	642:645	APIs	642:645	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	2	50	theme	active	607:612	arg1	ingredients					629:639	active pharmaceutical ingredients	607:639	active pharmaceutical ingredients (APIs)	607:646	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	2	50	theme	active	607:612	arg1	Acetaminophen					554:566	Acetaminophen	554:566	Acetaminophen (APAP)	554:573	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	1	51	theme	succinate	424:432	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	51	theme	succinate	424:432	arg1	126G					434:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	hydroxypropyl methylcellulose acetate succinate 126G	386:437	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	6	52	theme	polymers	1337:1344	arg1	hydrophilicity					1310:1323	hydrophilicity	1310:1323	hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP)	1310:1369	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	1	53	theme	long-term	156:164	arg1	stability					175:183	the long-term physical stability	152:183	the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH	152:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	3	54	theme	Gordon-Taylor	887:899	arg1	equation					909:916	the Gordon-Taylor or Kwei equation	883:916	equation	909:916	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	1	55	theme	%	548:548	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	55	theme	%	548:548	arg1	RH					550:551	40 °C/75% RH	540:551	40 °C/75% RH	540:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	4	56	from	solubility	962:971	arg1	polymers					992:999	the polymers	988:999	the polymers	988:999	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	7	57	theme	good	1420:1423	arg1	agreement					1425:1433	very good agreement	1415:1433	very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability	1415:1633	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	1	58	theme	amorphous	188:196	arg1	formulations					221:232	amorphous solid dispersion (ASD) formulations	188:232	amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH	188:551	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	4	59	theme	stabilizing	1017:1027	arg1	ability					1029:1035	the kinetic stabilizing ability	1005:1035	the kinetic stabilizing ability of the polymers	1005:1051	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	7	60	theme	experimental	1376:1387	arg1	results					1399:1405	The experimental stability results	1372:1405	The experimental stability results	1372:1405	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	3	61	theme	stability	663:671	arg1	studies					673:679	18 month long stability studies	649:679	18 month long stability studies of these formulations	649:701	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	0	62	theme	Long-Term	52:60	arg1	Stability					71:79	the Long-Term Physical Stability	48:79	the Long-Term Physical Stability of Amorphous Solid Dispersions	48:110	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.
29050468	7	63	theme	appropriate	1544:1554	arg1	compositions					1560:1571	appropriate ASD compositions	1544:1571	appropriate ASD compositions	1544:1571	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	1	64	theme	%	530:530	arg1	RH					532:533	25 °C/60% RH	522:533	25 °C/60% RH	522:533	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	64	theme	%	530:530	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	3	65	theme	month	652:656	arg1	studies					673:679	18 month long stability studies	649:679	18 month long stability studies of these formulations	649:701	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	1	66	theme	vinylpyrrolidone	302:317	arg1	PVP					325:327	PVP	325:327	PVP	325:327	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	66	theme	vinylpyrrolidone	302:317	arg1	HPMCAS					440:445	HPMCAS	440:445	HPMCAS	440:445	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	66	theme	vinylpyrrolidone	302:317	arg1	RH					517:518	RH	517:518	RH	517:518	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	66	theme	vinylpyrrolidone	302:317	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	66	theme	vinylpyrrolidone	302:317	arg1	humidity					507:514	25 °C/0% relative humidity	489:514	25 °C/0% relative humidity	489:514	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	66	theme	vinylpyrrolidone	302:317	arg1	K25					320:322	poly(vinylpyrrolidone) K25	297:322	poly(vinylpyrrolidone) K25 (PVP)	297:328	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	6	67	theme	PVPVA64	1356:1362	arg1	PVP					1366:1368	HPMCAS < PVPVA64 < PVP	1347:1368	HPMCAS < PVPVA64 < PVP	1347:1368	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	6	67	theme	PVPVA64	1356:1362	arg1	polymers					1337:1344	the pure polymers	1328:1344	the pure polymers (HPMCAS < PVPVA64 < PVP)	1328:1369	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	3	68	theme	phase	751:755	arg1	diagrams					757:764	the API/polymer phase diagrams	735:764	the API/polymer phase diagrams	735:764	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	4	69	theme	following	1069:1077	arg1	order					1079:1083	the following order	1065:1083	the following order	1065:1083	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	3	70	theme	formulations	690:701	arg1	studies					673:679	18 month long stability studies	649:679	18 month long stability studies of these formulations	649:701	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	0	71	theme	Amorphous	84:92	arg1	Dispersions					100:110	Amorphous Solid Dispersions	84:110	Amorphous Solid Dispersions	84:110	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.
29050468	7	72	theme	predictive	1511:1520	arg1	tools					1522:1526	suitable predictive tools	1502:1526	suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability	1502:1633	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	7	72	theme	predictive	1511:1520	arg1	PC-SAFT					1468:1474	PC-SAFT	1468:1474	PC-SAFT	1468:1474	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	7	72	theme	predictive	1511:1520	arg1	equation					1489:1496	the Kwei equation	1480:1496	the Kwei equation	1480:1496	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	0	73	theme	Dispersions	100:110	arg1	Stability					71:79	the Long-Term Physical Stability	48:79	the Long-Term Physical Stability of Amorphous Solid Dispersions	48:110	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.
29050468	4	74	theme	polymers	1044:1051	arg1	solubility					962:971	the solubility	958:971	the solubility of the APIs in the polymers	958:999	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	4	74	theme	polymers	1044:1051	arg1	ability					1029:1035	the kinetic stabilizing ability	1005:1035	the kinetic stabilizing ability of the polymers	1005:1051	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	1	75	theme	°C/0	492:495	arg1	humidity					507:514	25 °C/0% relative humidity	489:514	25 °C/0% relative humidity	489:514	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	75	theme	°C/0	492:495	arg1	K25					320:322	poly(vinylpyrrolidone) K25	297:322	poly(vinylpyrrolidone) K25 (PVP)	297:328	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	7	76	with	agreement	1425:1433	arg1	predictions					1440:1450	predictions	1440:1450	predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability	1440:1633	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	7	77	theme	Kwei	1484:1487	arg1	tools					1522:1526	suitable predictive tools	1502:1526	suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability	1502:1633	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	7	77	theme	Kwei	1484:1487	arg1	PC-SAFT					1468:1474	PC-SAFT	1468:1474	PC-SAFT	1468:1474	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	7	77	theme	Kwei	1484:1487	arg1	equation					1489:1496	the Kwei equation	1480:1496	the Kwei equation	1480:1496	The experimental stability results were in very good agreement with predictions confirming that PC-SAFT and the Kwei equation are suitable predictive tools for determining appropriate ASD compositions and storage conditions to ensure long-term physical stability.
29050468	1	78	theme	relative	498:505	arg1	humidity					507:514	25 °C/0% relative humidity	489:514	25 °C/0% relative humidity	489:514	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	78	theme	relative	498:505	arg1	K25					320:322	poly(vinylpyrrolidone) K25	297:322	poly(vinylpyrrolidone) K25 (PVP)	297:328	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	2	79	used	used	599:602	arg2	APAP					569:572	APAP	569:572	APAP	569:572	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	2	79	used	used	599:602	arg2	Acetaminophen					554:566	Acetaminophen	554:566	Acetaminophen (APAP)	554:573	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	2	79	used	used	599:602	arg2	ingredients					629:639	active pharmaceutical ingredients	607:639	active pharmaceutical ingredients (APIs)	607:646	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	2	79	used	used	599:602	arg2	NAP					589:591	NAP	589:591	NAP	589:591	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	2	79	used	used	599:602	arg2	naproxen					579:586	naproxen	579:586	naproxen (NAP)	579:592	Acetaminophen (APAP) and naproxen (NAP) were used as active pharmaceutical ingredients (APIs).
29050468	0	80	theme	Type	18:21	arg1	Impact					0:5	Impact	0:5	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.	0:111	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.
29050468	5	81	theme	kinetic	1139:1145	arg1	stabilization					1147:1159	the kinetic stabilization	1135:1159	the kinetic stabilization as well as NAP solubility	1135:1185	RH significantly reduces the kinetic stabilization as well as NAP solubility in the polymers, while the impact on APAP solubility is small.
29050468	0	82	theme	Relative	27:34	arg1	Humidity					36:43	Relative Humidity	27:43	Relative Humidity	27:43	Impact of Polymer Type and Relative Humidity on the Long-Term Physical Stability of Amorphous Solid Dispersions.
29050468	3	83	theme	Statistical	832:842	arg1	PC-SAFT					870:876	PC-SAFT	870:876	PC-SAFT	870:876	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	3	83	theme	Statistical	832:842	arg1	Theory					862:867	the Perturbed-Chain Statistical Associating Fluid Theory	812:867	the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT)	812:877	18 month long stability studies of these formulations were analyzed and compared with the API/polymer phase diagrams, which were modeled and predicted by applying the Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) and the Gordon-Taylor or Kwei equation.
29050468	6	84	from	impact	1254:1259	arg1	stability					1274:1282	the stability	1270:1282	the stability	1270:1282	The impact of RH on the stability increases with increasing hydrophilicity of the pure polymers (HPMCAS < PVPVA64 < PVP).
29050468	1	85	theme	poly	297:300	arg1	PVP					325:327	PVP	325:327	PVP	325:327	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	85	theme	poly	297:300	arg1	HPMCAS					440:445	HPMCAS	440:445	HPMCAS	440:445	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	85	theme	poly	297:300	arg1	RH					517:518	RH	517:518	RH	517:518	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	85	theme	poly	297:300	arg1	excipients					277:286	three different commercially used excipients	243:286	three different commercially used excipients	243:286	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	85	theme	poly	297:300	arg1	humidity					507:514	25 °C/0% relative humidity	489:514	25 °C/0% relative humidity	489:514	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	85	theme	poly	297:300	arg1	K25					320:322	poly(vinylpyrrolidone) K25	297:322	poly(vinylpyrrolidone) K25 (PVP)	297:328	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	1	86	theme	standardized	452:463	arg1	conditions					477:486	standardized ICH storage conditions	452:486	standardized ICH storage conditions	452:486	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
29050468	4	87	from	polymers	992:999	arg1	solubility					962:971	the solubility	958:971	the solubility of the APIs in the polymers	958:999	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	4	87	from	polymers	992:999	arg1	ability					1029:1035	the kinetic stabilizing ability	1005:1035	the kinetic stabilizing ability of the polymers	1005:1051	The study showed that, at dry storage, the solubility of the APIs in the polymers and the kinetic stabilizing ability of the polymers increase in the following order: HPMCAS < PVPVA64 < PVP.
29050468	1	88	theme	storage	469:475	arg1	conditions					477:486	standardized ICH storage conditions	452:486	standardized ICH storage conditions	452:486	The purpose of this work is to compare the long-term physical stability of amorphous solid dispersion (ASD) formulations based on three different commercially used excipients, namely, poly(vinylpyrrolidone) K25 (PVP), poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA64), and hydroxypropyl methylcellulose acetate succinate 126G (HPMCAS), at standardized ICH storage conditions, 25 °C/0% relative humidity (RH), 25 °C/60% RH, and 40 °C/75% RH.
24835578	8	0	theme	first	699:703	arg1	This					687:690	This	687:690	This	687:690	This is the first study to report the formation of vesicles containing 100% GSL.
24835578	8	0	theme	first	699:703	arg1	study					705:709	the first study	695:709	the first study to report the formation of vesicles containing 100% GSL	695:765	This is the first study to report the formation of vesicles containing 100% GSL.
24835578	1	1	theme	critical	178:185	arg1	role					187:190	a critical role	176:190	a critical role	176:190	Bacterial outer membrane components play a critical role in bacteria-surface interactions (adhesion and repulsion).
24835578	10	2	from	accumulation	1057:1068	arg1	sensor					1077:1082	the sensor	1073:1082	the sensor	1073:1082	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	11	3	theme	obtaining	1191:1199	arg1	ratio					1214:1218	the vesicle obtaining viscoelastic ratio	1179:1218	the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles	1179:1266	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	10	4	with	microbalance	934:945	arg1	technology					983:992	dissipation monitoring (QCM-D) technology	952:992	dissipation monitoring (QCM-D) technology	952:992	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	0	5	theme	viscoelastic	110:121	arg1	properties					123:132	viscoelastic properties	110:132	viscoelastic properties	110:132	Interactions of glycosphingolipids and lipopolysaccharides with silica and polyamide surfaces: adsorption and viscoelastic properties.
24835578	7	6	theme	GSL	657:659	arg1	vesicles					661:668	GSL vesicles	657:668	GSL vesicles	657:668	and to explain the adhesion of these cells to surfaces, we employed a single-component-based approach of comparing GSL vesicles to LPS vesicles.
24835578	11	7	theme	above	1127:1131	arg1	presence					1103:1110	the presence	1099:1110	the presence of 10% GSL and above	1099:1131	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	11	8	theme	vesicle	1183:1189	arg1	ratio					1214:1218	the vesicle obtaining viscoelastic ratio	1179:1218	the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles	1179:1266	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	11	9	theme	ratio	1214:1218	arg1	rigidity					1167:1174	the relative rigidity	1154:1174	the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles	1154:1266	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	10	10	theme	monitoring	964:973	arg1	technology					983:992	dissipation monitoring (QCM-D) technology	952:992	dissipation monitoring (QCM-D) technology	952:992	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	10	11	theme	quartz	919:924	arg1	microbalance					934:945	quartz crystal microbalance	919:945	quartz crystal microbalance with dissipation monitoring (QCM-D) technology	919:992	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	6	12	theme	spp	537:539	arg1	membrane					512:519	the outer membrane	502:519	the outer membrane of Sphingomonas spp	502:539	To delineate the properties of the outer membrane of Sphingomonas spp.
24835578	10	13	theme	Composition-dependent	851:871	arg1	adherence					881:889	Composition-dependent vesicle adherence	851:889	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology	851:992	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	10	14	theme	dissipation	952:962	arg1	technology					983:992	dissipation monitoring (QCM-D) technology	952:992	dissipation monitoring (QCM-D) technology	952:992	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	6	15	theme	Sphingomonas	524:535	arg1	spp					537:539	Sphingomonas spp	524:539	Sphingomonas spp	524:539	To delineate the properties of the outer membrane of Sphingomonas spp.
24835578	1	16	theme	bacteria-surface	195:210	arg1	interactions					212:223	bacteria-surface interactions	195:223	bacteria-surface interactions (adhesion and repulsion)	195:248	Bacterial outer membrane components play a critical role in bacteria-surface interactions (adhesion and repulsion).
24835578	1	16	theme	bacteria-surface	195:210	arg1	repulsion					239:247	repulsion	239:247	repulsion	239:247	Bacterial outer membrane components play a critical role in bacteria-surface interactions (adhesion and repulsion).
24835578	1	16	theme	bacteria-surface	195:210	arg1	adhesion					226:233	adhesion	226:233	adhesion	226:233	Bacterial outer membrane components play a critical role in bacteria-surface interactions (adhesion and repulsion).
24835578	7	17	theme	single-component-based	612:633	arg1	approach					635:642	a single-component-based approach	610:642	a single-component-based approach of comparing GSL vesicles to LPS vesicles	610:684	and to explain the adhesion of these cells to surfaces, we employed a single-component-based approach of comparing GSL vesicles to LPS vesicles.
24835578	7	18	theme	cells	579:583	arg1	adhesion					561:568	the adhesion	557:568	the adhesion of these cells to surfaces	557:595	and to explain the adhesion of these cells to surfaces, we employed a single-component-based approach of comparing GSL vesicles to LPS vesicles.
24835578	11	19	theme	%	1228:1228	arg1	ratio					1214:1218	the vesicle obtaining viscoelastic ratio	1179:1218	the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles	1179:1266	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	3	20	theme	Gram-negative	297:309	arg1	bacteria					311:318	other Gram-negative bacteria	291:318	other Gram-negative bacteria in that they lack lipopolysaccharides (LPSs) in their outer membrane	291:387	differ from other Gram-negative bacteria in that they lack lipopolysaccharides (LPSs) in their outer membrane.
24835578	11	21	theme	viscoelastic	1201:1212	arg1	ratio					1214:1218	the vesicle obtaining viscoelastic ratio	1179:1218	the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles	1179:1266	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	10	22	theme	higher	1014:1019	arg1	content					1025:1031	higher GSL content	1014:1031	higher GSL content	1014:1031	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	10	23	theme	vesicle	873:879	arg1	adherence					881:889	Composition-dependent vesicle adherence	851:889	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology	851:992	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	0	24	theme	glycosphingolipids	16:33	arg1	Interactions					0:11	Interactions	0:11	Interactions of glycosphingolipids and lipopolysaccharides with silica and polyamide surfaces: adsorption and viscoelastic properties.	0:133	Interactions of glycosphingolipids and lipopolysaccharides with silica and polyamide surfaces: adsorption and viscoelastic properties.
24835578	6	25	theme	membrane	512:519	arg1	properties					488:497	the properties	484:497	the properties of the outer membrane of Sphingomonas spp	484:539	To delineate the properties of the outer membrane of Sphingomonas spp.
24835578	10	26	theme	QCM-D	976:980	arg1	technology					983:992	dissipation monitoring (QCM-D) technology	952:992	dissipation monitoring (QCM-D) technology	952:992	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	9	27	dep	GSL	816:818	arg1	vesicles					828:835	vesicles	828:835	vesicles	828:835	Significant physicochemical differences between GSL and LPS vesicles are reported.
24835578	10	28	theme	different	894:902	arg1	surfaces					904:911	different surfaces	894:911	different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology	894:992	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	6	29	theme	outer	506:510	arg1	membrane					512:519	the outer membrane	502:519	the outer membrane of Sphingomonas spp	502:539	To delineate the properties of the outer membrane of Sphingomonas spp.
24835578	1	30	dep	interactions	212:223	arg1	interactions					212:223	bacteria-surface interactions	195:223	bacteria-surface interactions (adhesion and repulsion)	195:248	Bacterial outer membrane components play a critical role in bacteria-surface interactions (adhesion and repulsion).
24835578	1	30	dep	interactions	212:223	arg1	repulsion					239:247	repulsion	239:247	repulsion	239:247	Bacterial outer membrane components play a critical role in bacteria-surface interactions (adhesion and repulsion).
24835578	1	30	dep	interactions	212:223	arg1	adhesion					226:233	adhesion	226:233	adhesion	226:233	Bacterial outer membrane components play a critical role in bacteria-surface interactions (adhesion and repulsion).
24835578	11	31	theme	10	1115:1116	arg1	%					1117:1117	%	1117:1117	%	1117:1117	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	0	32	theme	lipopolysaccharides	39:57	arg1	Interactions					0:11	Interactions	0:11	Interactions of glycosphingolipids and lipopolysaccharides with silica and polyamide surfaces: adsorption and viscoelastic properties.	0:133	Interactions of glycosphingolipids and lipopolysaccharides with silica and polyamide surfaces: adsorption and viscoelastic properties.
24835578	8	33	theme	vesicles	738:745	arg1	formation					725:733	the formation	721:733	the formation of vesicles containing 100% GSL	721:765	This is the first study to report the formation of vesicles containing 100% GSL.
24835578	3	34	theme	other	291:295	arg1	bacteria					311:318	other Gram-negative bacteria	291:318	other Gram-negative bacteria in that they lack lipopolysaccharides (LPSs) in their outer membrane	291:387	differ from other Gram-negative bacteria in that they lack lipopolysaccharides (LPSs) in their outer membrane.
24835578	10	35	theme	mass	1052:1055	arg1	accumulation					1057:1068	higher mass accumulation	1045:1068	higher mass accumulation on the sensor	1045:1082	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	0	36	dep	silica	64:69	arg1	surfaces					85:92	surfaces	85:92	surfaces	85:92	Interactions of glycosphingolipids and lipopolysaccharides with silica and polyamide surfaces: adsorption and viscoelastic properties.
24835578	8	37	contain	containing	747:756	arg1	vesicles					738:745	vesicles	738:745	vesicles containing 100% GSL	738:765	This is the first study to report the formation of vesicles containing 100% GSL.
24835578	8	37	contain	containing	747:756	arg2	GSL					763:765	100% GSL	758:765	100% GSL	758:765	This is the first study to report the formation of vesicles containing 100% GSL.
24835578	2	38	dep	species	264:270	arg1	spp					273:275	spp	273:275	spp.	273:276	Sphingomonas species (spp.)
24835578	3	39	theme	outer	374:378	arg1	membrane					380:387	their outer membrane	368:387	their outer membrane	368:387	differ from other Gram-negative bacteria in that they lack lipopolysaccharides (LPSs) in their outer membrane.
24835578	11	40	theme	LPS	1255:1257	arg1	vesicles					1259:1266	pure LPS vesicles	1250:1266	pure LPS vesicles	1250:1266	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	10	41	theme	higher	1045:1050	arg1	accumulation					1057:1068	higher mass accumulation	1045:1068	higher mass accumulation on the sensor	1045:1082	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	2	42	theme	Sphingomonas	251:262	arg1	species					264:270	Sphingomonas species	251:270	Sphingomonas species (spp.)	251:277	Sphingomonas species (spp.)
24835578	1	43	theme	Bacterial	135:143	arg1	components					160:169	Bacterial outer membrane components	135:169	Bacterial outer membrane components	135:169	Bacterial outer membrane components play a critical role in bacteria-surface interactions (adhesion and repulsion).
24835578	11	44	theme	higher	1230:1235	arg1	%					1228:1228	30-70%	1223:1228	30-70% higher than that of pure LPS vesicles	1223:1266	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	8	45	theme	100	758:760	arg1	%					761:761	%	761:761	%	761:761	This is the first study to report the formation of vesicles containing 100% GSL.
24835578	3	46	from	lipopolysaccharides	338:356	arg1	membrane					380:387	their outer membrane	368:387	their outer membrane	368:387	differ from other Gram-negative bacteria in that they lack lipopolysaccharides (LPSs) in their outer membrane.
24835578	9	47	theme	Significant	768:778	arg1	differences					796:806	Significant physicochemical differences	768:806	Significant physicochemical differences between GSL and LPS vesicles	768:835	Significant physicochemical differences between GSL and LPS vesicles are reported.
24835578	1	48	theme	outer	145:149	arg1	components					160:169	Bacterial outer membrane components	135:169	Bacterial outer membrane components	135:169	Bacterial outer membrane components play a critical role in bacteria-surface interactions (adhesion and repulsion).
24835578	10	49	theme	GSL	1021:1023	arg1	content					1025:1031	higher GSL content	1014:1031	higher GSL content	1014:1031	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
24835578	11	50	theme	relative	1158:1165	arg1	rigidity					1167:1174	the relative rigidity	1154:1174	the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles	1154:1266	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	9	51	theme	physicochemical	780:794	arg1	differences					796:806	Significant physicochemical differences	768:806	Significant physicochemical differences between GSL and LPS vesicles	768:835	Significant physicochemical differences between GSL and LPS vesicles are reported.
24835578	0	52	with	Interactions	0:11	arg1	polyamide					75:83	polyamide	75:83	polyamide	75:83	Interactions of glycosphingolipids and lipopolysaccharides with silica and polyamide surfaces: adsorption and viscoelastic properties.
24835578	0	52	with	Interactions	0:11	arg1	silica					64:69	silica	64:69	silica	64:69	Interactions of glycosphingolipids and lipopolysaccharides with silica and polyamide surfaces: adsorption and viscoelastic properties.
24835578	1	53	theme	membrane	151:158	arg1	components					160:169	Bacterial outer membrane components	135:169	Bacterial outer membrane components	135:169	Bacterial outer membrane components play a critical role in bacteria-surface interactions (adhesion and repulsion).
24835578	11	54	theme	pure	1250:1253	arg1	vesicles					1259:1266	pure LPS vesicles	1250:1266	pure LPS vesicles	1250:1266	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	7	55	theme	LPS	673:675	arg1	vesicles					677:684	LPS vesicles	673:684	LPS vesicles	673:684	and to explain the adhesion of these cells to surfaces, we employed a single-component-based approach of comparing GSL vesicles to LPS vesicles.
24835578	8	56	theme	%	761:761	arg1	GSL					763:765	100% GSL	758:765	100% GSL	758:765	This is the first study to report the formation of vesicles containing 100% GSL.
24835578	11	57	theme	%	1117:1117	arg1	GSL					1119:1121	10% GSL	1115:1121	10% GSL	1115:1121	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	11	58	theme	GSL	1119:1121	arg1	presence					1103:1110	the presence	1099:1110	the presence of 10% GSL and above	1099:1131	Additionally, the presence of 10% GSL and above was found to promote the relative rigidity of the vesicle obtaining viscoelastic ratio of 30-70% higher than that of pure LPS vesicles.
24835578	5	59	theme	outer	417:421	arg1	membrane					423:430	outer membrane	417:430	outer membrane	417:430	outer membrane consists of glycosphingolipids (GSLs).
24835578	0	60	dep	Interactions	0:11	arg1	adsorption					95:104	adsorption	95:104	adsorption	95:104	Interactions of glycosphingolipids and lipopolysaccharides with silica and polyamide surfaces: adsorption and viscoelastic properties.
24835578	0	60	dep	Interactions	0:11	arg1	properties					123:132	viscoelastic properties	110:132	viscoelastic properties	110:132	Interactions of glycosphingolipids and lipopolysaccharides with silica and polyamide surfaces: adsorption and viscoelastic properties.
24835578	10	61	theme	crystal	926:932	arg1	microbalance					934:945	quartz crystal microbalance	919:945	quartz crystal microbalance with dissipation monitoring (QCM-D) technology	919:992	Composition-dependent vesicle adherence to different surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D) technology was observed, where higher GSL content resulted in higher mass accumulation on the sensor.
29177720	3	0	dep	temperature	649:659	arg1	30 °C					669:673	30 °C	669:673	30 °C	669:673	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	3	0	dep	temperature	649:659	arg1	37 °C					680:684	37 °C	680:684	37 °C	680:684	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	3	0	dep	temperature	649:659	arg1	22 °C					662:666	22 °C	662:666	22 °C	662:666	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	4	1	theme	GM	756:757	arg1	cultures					769:776	GM C41 (DE3) cultures	756:776	GM C41 (DE3) cultures (3 h after IPTG induction)	756:803	BC biosynthesis was detected much earlier in GM C41 (DE3) cultures (3 h after IPTG induction) than those of Gluconacetobacter hansenii.
29177720	0	2	from	biosynthesis	12:23	arg1	coli					84:87	Escherichia coli	72:87	genetically modified Escherichia coli	51:87	Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli.
29177720	4	3	theme	C41	759:761	arg1	cultures					769:776	GM C41 (DE3) cultures	756:776	GM C41 (DE3) cultures (3 h after IPTG induction)	756:803	BC biosynthesis was detected much earlier in GM C41 (DE3) cultures (3 h after IPTG induction) than those of Gluconacetobacter hansenii.
29177720	3	4	theme	concentration	696:708	arg1	optimization					621:632	optimization	621:632	optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration	621:708	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	2	5	theme	Ax	547:548	arg1	ccp					543:545	ccp Ax	543:548	ccp Ax	543:548	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	5	6	theme	10-20 μm	949:956	arg1	length					894:899	a length	892:899	a length of approximately 1000-3000 μm	892:929	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	5	6	theme	10-20 μm	949:956	arg1	diameter					937:944	a diameter	935:944	a diameter of 10-20 μm	935:956	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	3	7	theme	IPTG	691:694	arg1	concentration					696:708	IPTG concentration	691:708	IPTG concentration	691:708	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	2	8	theme	operon	494:499	arg1	expression					459:468	recombinant expression	447:468	recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax)	447:549	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	1	9	theme	Bacterial	90:98	arg1	BC					111:112	BC	111:112	BC	111:112	Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria.
29177720	1	9	theme	Bacterial	90:98	arg1	cellulose					100:108	Bacterial cellulose	90:108	Bacterial cellulose (BC)	90:113	Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria.
29177720	1	10	theme	plant-based	174:184	arg1	cellulose					186:194	plant-based cellulose	174:194	plant-based cellulose	174:194	Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria.
29177720	3	11	theme	temperature	649:659	arg1	optimization					621:632	optimization	621:632	optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration	621:708	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	2	12	dep	cmcax	536:540	arg1	ccp					543:545	ccp Ax	543:548	ccp Ax	543:548	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	2	13	theme	BC	377:378	arg1	system					391:396	a functional and stable BC production system	353:396	a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax)	353:549	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	0	14	theme	Recombinant	0:10	arg1	biosynthesis					12:23	Recombinant biosynthesis	0:23	Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli	0:87	Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli.
29177720	2	15	dep	Escherichia	427:437	arg1	coli					439:442	coli	439:442	coli	439:442	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	2	16	theme	stable	370:375	arg1	system					391:396	a functional and stable BC production system	353:396	a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax)	353:549	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	0	17	theme	bacterial	28:36	arg1	cellulose					38:46	bacterial cellulose	28:46	bacterial cellulose	28:46	Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli.
29177720	2	18	theme	operon	528:533	arg1	expression					459:468	recombinant expression	447:468	recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax)	447:549	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	3	19	theme	culture	641:647	arg1	temperature					649:659	the culture temperature	637:659	the culture temperature (22 °C, 30 °C, and 37 °C)	637:685	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	2	20	theme	upstream	519:526	arg1	cmcax					536:540	cmcax	536:540	cmcax	536:540	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	2	20	theme	upstream	519:526	arg1	operon					528:533	the upstream operon	515:533	the upstream operon (cmcax, ccp Ax)	515:549	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	5	21	theme	1000-3000 μm	918:929	arg1	length					894:899	a length	892:899	a length of approximately 1000-3000 μm	892:929	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	5	21	theme	1000-3000 μm	918:929	arg1	diameter					937:944	a diameter	935:944	a diameter of 10-20 μm	935:956	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	5	22	theme	G.	1032:1033	arg1	hansenii					1035:1042	G. hansenii	1032:1042	G. hansenii	1032:1042	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	1	23	theme	strain	281:286	arg1	properties					288:297	the strain properties	277:297	the strain properties of cellulose-producing bacteria	277:329	Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria.
29177720	1	24	theme	commercial	206:215	arg1	production					217:226	commercial production	206:226	commercial production of BC	206:232	Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria.
29177720	0	25	theme	cellulose	38:46	arg1	biosynthesis					12:23	Recombinant biosynthesis	0:23	Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli	0:87	Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli.
29177720	4	26	theme	IPTG	789:792	arg1	induction					794:802	IPTG induction	789:802	IPTG induction	789:802	BC biosynthesis was detected much earlier in GM C41 (DE3) cultures (3 h after IPTG induction) than those of Gluconacetobacter hansenii.
29177720	3	27	theme	GM	582:583	arg1	DE3					593:595	DE3	593:595	DE3	593:595	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	3	27	theme	GM	582:583	arg1	HMS174					585:590	GM HMS174	582:590	GM HMS174 (DE3)	582:596	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	3	28	theme	BC	552:553	arg1	production					555:564	BC production	552:564	BC production	552:564	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	5	29	theme	BC	1007:1008	arg1	fibres					997:1002	the fibres	993:1002	the fibres of BC typically produced by G. hansenii	993:1042	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	2	30	theme	recombinant	447:457	arg1	expression					459:468	recombinant expression	447:468	recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax)	447:549	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	4	31	theme	Gluconacetobacter	819:835	arg1	hansenii					837:844	Gluconacetobacter hansenii	819:844	Gluconacetobacter hansenii	819:844	BC biosynthesis was detected much earlier in GM C41 (DE3) cultures (3 h after IPTG induction) than those of Gluconacetobacter hansenii.
29177720	1	32	theme	cellulose-producing	302:320	arg1	bacteria					322:329	cellulose-producing bacteria	302:329	cellulose-producing bacteria	302:329	Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria.
29177720	4	33	theme	DE3	764:766	arg1	cultures					769:776	GM C41 (DE3) cultures	756:776	GM C41 (DE3) cultures (3 h after IPTG induction)	756:803	BC biosynthesis was detected much earlier in GM C41 (DE3) cultures (3 h after IPTG induction) than those of Gluconacetobacter hansenii.
29177720	4	34	theme	BC	711:712	arg1	biosynthesis					714:725	BC biosynthesis	711:725	BC biosynthesis	711:725	BC biosynthesis was detected much earlier in GM C41 (DE3) cultures (3 h after IPTG induction) than those of Gluconacetobacter hansenii.
29177720	2	35	from	system	391:396	arg1	GM					423:424	GM	423:424	GM	423:424	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	2	35	from	system	391:396	arg1	modified					413:420	modified	413:420	modified	413:420	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	1	36	theme	unique	124:129	arg1	properties					131:140	unique properties	124:140	unique properties such as high purity	124:160	Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria.
29177720	1	36	theme	unique	124:129	arg1	purity					155:160	high purity	150:160	high purity	150:160	Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria.
29177720	5	37	contain	having	885:890	arg2	diameter					937:944	a diameter	935:944	a diameter of 10-20 μm	935:956	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	5	37	contain	having	885:890	arg1	fibres					878:883	dense fibres	872:883	dense fibres	872:883	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	5	37	contain	having	885:890	arg2	length					894:899	a length	892:899	a length of approximately 1000-3000 μm	892:929	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	1	38	theme	bacteria	322:329	arg1	properties					288:297	the strain properties	277:297	the strain properties of cellulose-producing bacteria	277:329	Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria.
29177720	3	39	theme	GM	605:606	arg1	DE3					613:615	DE3	613:615	DE3	613:615	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	3	39	theme	GM	605:606	arg1	C41					608:610	GM C41	605:610	GM C41 (DE3)	605:616	BC production was achieved in GM HMS174 (DE3) and in GM C41 (DE3) by optimization of the culture temperature (22 °C, 30 °C, and 37 °C) and IPTG concentration.
29177720	5	40	theme	dense	872:876	arg1	fibres					878:883	dense fibres	872:883	dense fibres	872:883	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	5	41	theme	GM	847:848	arg1	DE3					858:860	DE3	858:860	DE3	858:860	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	5	41	theme	GM	847:848	arg1	HMS174					850:855	GM HMS174	847:855	GM HMS174 (DE3)	847:861	GM HMS174 (DE3) produced dense fibres having a length of approximately 1000-3000 μm and a diameter of 10-20 μm, which were remarkably larger than the fibres of BC typically produced by G. hansenii.
29177720	1	42	theme	high	150:153	arg1	purity					155:160	high purity	150:160	high purity	150:160	Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria.
29177720	1	43	theme	BC	231:232	arg1	production					217:226	commercial production	206:226	commercial production of BC	206:232	Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria.
29177720	2	44	theme	synthase	485:492	arg1	bcsABCD					502:508	bcsABCD	502:508	bcsABCD	502:508	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	2	44	theme	synthase	485:492	arg1	operon					494:499	the BC synthase operon	478:499	the BC synthase operon (bcsABCD)	478:509	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	2	45	theme	production	380:389	arg1	system					391:396	a functional and stable BC production system	353:396	a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax)	353:549	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	2	46	theme	BC	482:483	arg1	bcsABCD					502:508	bcsABCD	502:508	bcsABCD	502:508	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	2	46	theme	BC	482:483	arg1	operon					494:499	the BC synthase operon	478:499	the BC synthase operon (bcsABCD)	478:509	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
29177720	4	47	dep	cultures	769:776	arg1	3 h					779:781	3 h	779:781	3 h after IPTG induction	779:802	BC biosynthesis was detected much earlier in GM C41 (DE3) cultures (3 h after IPTG induction) than those of Gluconacetobacter hansenii.
29177720	2	48	theme	functional	355:364	arg1	system					391:396	a functional and stable BC production system	353:396	a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax)	353:549	Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC synthase operon (bcsABCD) and the upstream operon (cmcax, ccp Ax).
25498623	5	0	theme	increasing	712:721	arg1	haze					723:726	a brown color and increasing haze	694:726	haze	723:726	The resulting films presented a brown color and increasing haze with chitosan plasticized content.
25498623	3	1	theme	ethylene	466:473	arg1	EVA					490:492	EVA	490:492	EVA	490:492	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	3	1	theme	ethylene	466:473	arg1	acetate					481:487	ethylene vinyl acetate	466:487	ethylene vinyl acetate	466:487	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	3	2	theme	vinyl	475:479	arg1	EVA					490:492	EVA	490:492	EVA	490:492	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	3	2	theme	vinyl	475:479	arg1	acetate					481:487	ethylene vinyl acetate	466:487	ethylene vinyl acetate	466:487	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	3	3	theme	Different	365:373	arg1	dilutions					375:383	Different dilutions	365:383	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA,	365:493	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	3	4	dep	masterbatch	392:402	arg1	%					419:419	2, 5 and 10 wt%	405:419	2, 5 and 10 wt% of plasticized chitosan	405:443	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	3	4	dep	masterbatch	392:402	arg1	chitosan					436:443	plasticized chitosan	424:443	plasticized chitosan	424:443	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	1	5	theme	chitosan	73:80	arg1	blends					99:104	Plasticized chitosan and polyethylene blends	61:104	Plasticized chitosan and polyethylene blends	61:104	Plasticized chitosan and polyethylene blends were produced through a single-pass extrusion process.
25498623	4	6	from	visible	631:637	arg1	matrix					656:661	the polymeric matrix	642:661	the polymeric matrix	642:661	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	6	7	theme	Mechanical	763:772	arg1	properties					774:783	Mechanical properties	763:783	Mechanical properties of the mPE films	763:800	Mechanical properties of the mPE films were affected by the presence of plasticized chitosan, but improvement was observed as a result of some compatibility between mPE and chitosan in the presence of EVA.
25498623	4	8	theme	polymeric	646:654	arg1	matrix					656:661	the polymeric matrix	642:661	the polymeric matrix	642:661	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	2	9	theme	twin-screw	169:178	arg1	extruder					180:187	a twin-screw extruder	167:187	a twin-screw extruder	167:187	Using a twin-screw extruder, chitosan plasticization was achieved in the presence of an acetic acid solution and glycerol, and directly mixed with metallocene polyethylene, mPE, to produce a masterbatch.
25498623	1	10	theme	polyethylene	86:97	arg1	blends					99:104	Plasticized chitosan and polyethylene blends	61:104	Plasticized chitosan and polyethylene blends	61:104	Plasticized chitosan and polyethylene blends were produced through a single-pass extrusion process.
25498623	5	11	theme	brown	696:700	arg1	color					702:706	a brown color and increasing haze	694:726	color	702:706	The resulting films presented a brown color and increasing haze with chitosan plasticized content.
25498623	3	12	from	masterbatch	392:402	arg1	presence					454:461	the presence	450:461	the presence of ethylene vinyl acetate, EVA,	450:493	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	6	13	theme	EVA	964:966	arg1	presence					952:959	the presence	948:959	the presence of EVA	948:966	Mechanical properties of the mPE films were affected by the presence of plasticized chitosan, but improvement was observed as a result of some compatibility between mPE and chitosan in the presence of EVA.
25498623	2	14	dep	solution	261:268	arg1	the					230:232	the	230:232	the	230:232	Using a twin-screw extruder, chitosan plasticization was achieved in the presence of an acetic acid solution and glycerol, and directly mixed with metallocene polyethylene, mPE, to produce a masterbatch.
25498623	2	14	dep	solution	261:268	arg1	presence					234:241	presence	234:241	presence	234:241	Using a twin-screw extruder, chitosan plasticization was achieved in the presence of an acetic acid solution and glycerol, and directly mixed with metallocene polyethylene, mPE, to produce a masterbatch.
25498623	0	15	theme	chitosan/polyolefin	12:30	arg1	films					32:36	Plasticized chitosan/polyolefin films	0:36	Plasticized chitosan/polyolefin films	0:36	Plasticized chitosan/polyolefin films produced by extrusion.
25498623	7	16	theme	water	1032:1036	arg1	permeability					1044:1055	mPE water vapor permeability	1028:1055	mPE water vapor permeability	1028:1055	Finally the incorporation of plasticized chitosan affected mPE water vapor permeability while oxygen permeability remained constant.
25498623	3	17	theme	single	525:530	arg1	extrusion					543:551	single screw film extrusion	525:551	single screw film extrusion	525:551	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	6	18	theme	chitosan	847:854	arg1	presence					823:830	the presence	819:830	the presence of plasticized chitosan	819:854	Mechanical properties of the mPE films were affected by the presence of plasticized chitosan, but improvement was observed as a result of some compatibility between mPE and chitosan in the presence of EVA.
25498623	0	19	theme	Plasticized	0:10	arg1	films					32:36	Plasticized chitosan/polyolefin films	0:36	Plasticized chitosan/polyolefin films	0:36	Plasticized chitosan/polyolefin films produced by extrusion.
25498623	7	20	theme	vapor	1038:1042	arg1	permeability					1044:1055	mPE water vapor permeability	1028:1055	mPE water vapor permeability	1028:1055	Finally the incorporation of plasticized chitosan affected mPE water vapor permeability while oxygen permeability remained constant.
25498623	3	21	from	presence	454:461	arg1	dilutions					375:383	Different dilutions	365:383	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA,	365:493	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	3	22	theme	screw	532:536	arg1	extrusion					543:551	single screw film extrusion	525:551	single screw film extrusion	525:551	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	3	23	theme	wt	417:418	arg1	%					419:419	2, 5 and 10 wt%	405:419	2, 5 and 10 wt% of plasticized chitosan	405:443	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	3	23	theme	wt	417:418	arg1	chitosan					436:443	plasticized chitosan	424:443	plasticized chitosan	424:443	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	4	24	theme	number	595:600	arg1	μm					622:623	number average diameter <5 μm	595:623	number average diameter <5 μm	595:623	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	4	24	theme	number	595:600	arg1	domains					586:592	Very small plasticized chitosan domains	554:592	Very small plasticized chitosan domains (number average diameter <5 μm)	554:624	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	6	25	theme	compatibility	906:918	arg1	result					891:896	a result	889:896	a result of some compatibility between mPE and chitosan	889:943	Mechanical properties of the mPE films were affected by the presence of plasticized chitosan, but improvement was observed as a result of some compatibility between mPE and chitosan in the presence of EVA.
25498623	6	25	theme	compatibility	906:918	arg1	improvement					861:871	improvement	861:871	improvement	861:871	Mechanical properties of the mPE films were affected by the presence of plasticized chitosan, but improvement was observed as a result of some compatibility between mPE and chitosan in the presence of EVA.
25498623	7	26	theme	mPE	1028:1030	arg1	permeability					1044:1055	mPE water vapor permeability	1028:1055	mPE water vapor permeability	1028:1055	Finally the incorporation of plasticized chitosan affected mPE water vapor permeability while oxygen permeability remained constant.
25498623	2	27	theme	metallocene	308:318	arg1	polyethylene					320:331	metallocene polyethylene	308:331	metallocene polyethylene	308:331	Using a twin-screw extruder, chitosan plasticization was achieved in the presence of an acetic acid solution and glycerol, and directly mixed with metallocene polyethylene, mPE, to produce a masterbatch.
25498623	2	27	theme	metallocene	308:318	arg1	mPE					334:336	mPE	334:336	mPE	334:336	Using a twin-screw extruder, chitosan plasticization was achieved in the presence of an acetic acid solution and glycerol, and directly mixed with metallocene polyethylene, mPE, to produce a masterbatch.
25498623	6	28	located	observed	877:884	arg2	improvement					861:871	improvement	861:871	improvement	861:871	Mechanical properties of the mPE films were affected by the presence of plasticized chitosan, but improvement was observed as a result of some compatibility between mPE and chitosan in the presence of EVA.
25498623	6	28	located	observed	877:884	arg1	presence					952:959	the presence	948:959	the presence of EVA	948:966	Mechanical properties of the mPE films were affected by the presence of plasticized chitosan, but improvement was observed as a result of some compatibility between mPE and chitosan in the presence of EVA.
25498623	6	28	located	observed	877:884	arg2	result					891:896	a result	889:896	a result of some compatibility between mPE and chitosan	889:943	Mechanical properties of the mPE films were affected by the presence of plasticized chitosan, but improvement was observed as a result of some compatibility between mPE and chitosan in the presence of EVA.
25498623	7	29	theme	plasticized	998:1008	arg1	chitosan					1010:1017	plasticized chitosan	998:1017	plasticized chitosan	998:1017	Finally the incorporation of plasticized chitosan affected mPE water vapor permeability while oxygen permeability remained constant.
25498623	4	30	theme	diameter	610:617	arg1	μm					622:623	number average diameter <5 μm	595:623	number average diameter <5 μm	595:623	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	4	30	theme	diameter	610:617	arg1	domains					586:592	Very small plasticized chitosan domains	554:592	Very small plasticized chitosan domains (number average diameter <5 μm)	554:624	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	2	31	theme	acid	256:259	arg1	solution					261:268	an acetic acid solution	246:268	an acetic acid solution	246:268	Using a twin-screw extruder, chitosan plasticization was achieved in the presence of an acetic acid solution and glycerol, and directly mixed with metallocene polyethylene, mPE, to produce a masterbatch.
25498623	3	32	theme	plasticized	424:434	arg1	chitosan					436:443	plasticized chitosan	424:443	plasticized chitosan	424:443	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	5	33	theme	chitosan	733:740	arg1	content					754:760	chitosan plasticized content	733:760	chitosan plasticized content	733:760	The resulting films presented a brown color and increasing haze with chitosan plasticized content.
25498623	5	34	theme	resulting	668:676	arg1	films					678:682	The resulting films	664:682	The resulting films	664:682	The resulting films presented a brown color and increasing haze with chitosan plasticized content.
25498623	2	35	theme	chitosan	190:197	arg1	plasticization					199:212	chitosan plasticization	190:212	chitosan plasticization	190:212	Using a twin-screw extruder, chitosan plasticization was achieved in the presence of an acetic acid solution and glycerol, and directly mixed with metallocene polyethylene, mPE, to produce a masterbatch.
25498623	2	36	theme	acetic	249:254	arg1	solution					261:268	an acetic acid solution	246:268	an acetic acid solution	246:268	Using a twin-screw extruder, chitosan plasticization was achieved in the presence of an acetic acid solution and glycerol, and directly mixed with metallocene polyethylene, mPE, to produce a masterbatch.
25498623	3	37	theme	chitosan	436:443	arg1	%					419:419	2, 5 and 10 wt%	405:419	2, 5 and 10 wt% of plasticized chitosan	405:443	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	3	37	theme	chitosan	436:443	arg1	chitosan					436:443	plasticized chitosan	424:443	plasticized chitosan	424:443	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	5	38	theme	plasticized	742:752	arg1	content					754:760	chitosan plasticized content	733:760	chitosan plasticized content	733:760	The resulting films presented a brown color and increasing haze with chitosan plasticized content.
25498623	3	39	from	dilutions	375:383	arg1	presence					454:461	the presence	450:461	the presence of ethylene vinyl acetate, EVA,	450:493	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	3	40	theme	film	538:541	arg1	extrusion					543:551	single screw film extrusion	525:551	single screw film extrusion	525:551	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	6	41	theme	plasticized	835:845	arg1	chitosan					847:854	plasticized chitosan	835:854	plasticized chitosan	835:854	Mechanical properties of the mPE films were affected by the presence of plasticized chitosan, but improvement was observed as a result of some compatibility between mPE and chitosan in the presence of EVA.
25498623	1	42	theme	single-pass	130:140	arg1	process					152:158	a single-pass extrusion process	128:158	a single-pass extrusion process	128:158	Plasticized chitosan and polyethylene blends were produced through a single-pass extrusion process.
25498623	7	43	theme	chitosan	1010:1017	arg1	incorporation					981:993	the incorporation	977:993	the incorporation of plasticized chitosan	977:1017	Finally the incorporation of plasticized chitosan affected mPE water vapor permeability while oxygen permeability remained constant.
25498623	1	44	theme	extrusion	142:150	arg1	process					152:158	a single-pass extrusion process	128:158	a single-pass extrusion process	128:158	Plasticized chitosan and polyethylene blends were produced through a single-pass extrusion process.
25498623	6	45	theme	films	796:800	arg1	properties					774:783	Mechanical properties	763:783	Mechanical properties of the mPE films	763:800	Mechanical properties of the mPE films were affected by the presence of plasticized chitosan, but improvement was observed as a result of some compatibility between mPE and chitosan in the presence of EVA.
25498623	4	46	theme	plasticized	565:575	arg1	μm					622:623	number average diameter <5 μm	595:623	number average diameter <5 μm	595:623	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	4	46	theme	plasticized	565:575	arg1	domains					586:592	Very small plasticized chitosan domains	554:592	Very small plasticized chitosan domains (number average diameter <5 μm)	554:624	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	6	47	theme	mPE	792:794	arg1	films					796:800	the mPE films	788:800	the mPE films	788:800	Mechanical properties of the mPE films were affected by the presence of plasticized chitosan, but improvement was observed as a result of some compatibility between mPE and chitosan in the presence of EVA.
25498623	7	48	theme	oxygen	1063:1068	arg1	permeability					1070:1081	oxygen permeability	1063:1081	oxygen permeability	1063:1081	Finally the incorporation of plasticized chitosan affected mPE water vapor permeability while oxygen permeability remained constant.
25498623	4	49	theme	small	559:563	arg1	μm					622:623	number average diameter <5 μm	595:623	number average diameter <5 μm	595:623	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	4	49	theme	small	559:563	arg1	domains					586:592	Very small plasticized chitosan domains	554:592	Very small plasticized chitosan domains (number average diameter <5 μm)	554:624	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	4	50	theme	average	602:608	arg1	μm					622:623	number average diameter <5 μm	595:623	number average diameter <5 μm	595:623	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	4	50	theme	average	602:608	arg1	domains					586:592	Very small plasticized chitosan domains	554:592	Very small plasticized chitosan domains (number average diameter <5 μm)	554:624	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	3	51	theme	acetate	481:487	arg1	presence					454:461	the presence	450:461	the presence of ethylene vinyl acetate, EVA,	450:493	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	4	52	from	matrix	656:661	arg1	visible					631:637	visible	631:637	visible	631:637	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	1	53	theme	Plasticized	61:71	arg1	blends					99:104	Plasticized chitosan and polyethylene blends	61:104	Plasticized chitosan and polyethylene blends	61:104	Plasticized chitosan and polyethylene blends were produced through a single-pass extrusion process.
25498623	3	54	theme	masterbatch	392:402	arg1	dilutions					375:383	Different dilutions	365:383	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA,	365:493	Different dilutions of the masterbatch (2, 5 and 10 wt% of plasticized chitosan), in the presence of ethylene vinyl acetate, EVA, were subsequently achieved in single screw film extrusion.
25498623	4	55	theme	chitosan	577:584	arg1	μm					622:623	number average diameter <5 μm	595:623	number average diameter <5 μm	595:623	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
25498623	4	55	theme	chitosan	577:584	arg1	domains					586:592	Very small plasticized chitosan domains	554:592	Very small plasticized chitosan domains (number average diameter <5 μm)	554:624	Very small plasticized chitosan domains (number average diameter <5 μm) were visible in the polymeric matrix.
28214583	0	0	theme	hydrotalcite/chitosan	77:97	arg1	composite					99:107	hydrotalcite/chitosan composite	77:107	hydrotalcite/chitosan composite	77:107	Remediation of fluoride from drinking water using magnetic iron oxide coated hydrotalcite/chitosan composite.
28214583	2	1	theme	other	479:483	arg1	components					490:499	other base components	479:499	other base components	479:499	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	4	2	theme	mapping	781:787	arg1	techniques					789:798	mapping techniques	781:798	mapping techniques	781:798	The structure and morphology of the prepared adsorbent and fluoride sorbed adsorbent was analysed using FTIR, SEM and EDAX with mapping techniques.
28214583	8	3	theme	@	1216:1216	arg1	composite					1222:1230	Fe3O4@HTCS composite	1211:1230	Fe3O4@HTCS composite	1211:1230	The performance of Fe3O4@HTCS composite to field water sample designates its adaptable nature at field conditions.
28214583	7	4	theme	spontaneity	1129:1139	arg1	nature					1157:1162	the spontaneity and endothermic nature	1125:1162	the spontaneity and endothermic nature of the fluoride adsorption	1125:1189	Thermodynamic parameters confirm the spontaneity and endothermic nature of the fluoride adsorption.
28214583	5	5	theme	DC	819:820	arg1	dependence					805:814	The dependence	801:814	The dependence of DC on various parameters like initial fluoride concentration, pH, contact time, interfering anions and temperature	801:932	The dependence of DC on various parameters like initial fluoride concentration, pH, contact time, interfering anions and temperature was studied by batch method.
28214583	4	6	with	SEM	763:765	arg1	techniques					789:798	mapping techniques	781:798	mapping techniques	781:798	The structure and morphology of the prepared adsorbent and fluoride sorbed adsorbent was analysed using FTIR, SEM and EDAX with mapping techniques.
28214583	1	7	from	water	305:309	arg1	removal					256:262	the removal	252:262	the removal of excess fluoride content from drinking water	252:309	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	3	8	theme	Fe3O4	527:531	arg1	composite					558:566	Fe3O4@HT composite	549:566	Fe3O4@HT composite	549:566	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	8	theme	Fe3O4	527:531	arg1	CS					583:584	CS	583:584	CS	583:584	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	8	theme	Fe3O4	527:531	arg1	HTCS					533:536	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	8	theme	Fe3O4	527:531	arg1	composite					538:546	composite	538:546	composite	538:546	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	8	theme	Fe3O4	527:531	arg1	Fe3O4					569:573	Fe3O4	569:573	Fe3O4	569:573	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	8	theme	Fe3O4	527:531	arg1	HT					576:577	HT	576:577	HT	576:577	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	6	9	theme	Langmuir	1041:1048	arg1	isotherms					1081:1089	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1029:1089	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1029:1089	From isotherm modeling, the equilibrium data is well described by Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms.
28214583	5	10	theme	initial	849:855	arg1	concentration					866:878	initial fluoride concentration	849:878	initial fluoride concentration	849:878	The dependence of DC on various parameters like initial fluoride concentration, pH, contact time, interfering anions and temperature was studied by batch method.
28214583	6	11	theme	Dubinin-Radushkevich	1054:1073	arg1	isotherms					1081:1089	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1029:1089	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1029:1089	From isotherm modeling, the equilibrium data is well described by Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms.
28214583	8	12	theme	Fe3O4	1211:1215	arg1	composite					1222:1230	Fe3O4@HTCS composite	1211:1230	Fe3O4@HTCS composite	1211:1230	The performance of Fe3O4@HTCS composite to field water sample designates its adaptable nature at field conditions.
28214583	3	13	theme	@	532:532	arg1	composite					558:566	Fe3O4@HT composite	549:566	Fe3O4@HT composite	549:566	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	13	theme	@	532:532	arg1	CS					583:584	CS	583:584	CS	583:584	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	13	theme	@	532:532	arg1	HTCS					533:536	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	13	theme	@	532:532	arg1	composite					538:546	composite	538:546	composite	538:546	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	13	theme	@	532:532	arg1	Fe3O4					569:573	Fe3O4	569:573	Fe3O4	569:573	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	13	theme	@	532:532	arg1	HT					576:577	HT	576:577	HT	576:577	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	1	14	theme	excess	267:272	arg1	content					283:289	excess fluoride content	267:289	excess fluoride content	267:289	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	7	15	theme	Thermodynamic	1092:1104	arg1	parameters					1106:1115	Thermodynamic parameters	1092:1115	Thermodynamic parameters	1092:1115	Thermodynamic parameters confirm the spontaneity and endothermic nature of the fluoride adsorption.
28214583	7	16	theme	fluoride	1171:1178	arg1	adsorption					1180:1189	the fluoride adsorption	1167:1189	the fluoride adsorption	1167:1189	Thermodynamic parameters confirm the spontaneity and endothermic nature of the fluoride adsorption.
28214583	5	17	from	dependence	805:814	arg1	parameters					833:842	various parameters	825:842	various parameters like initial fluoride concentration, pH, contact time, interfering anions and temperature	825:932	The dependence of DC on various parameters like initial fluoride concentration, pH, contact time, interfering anions and temperature was studied by batch method.
28214583	4	18	theme	adsorbent	698:706	arg1	morphology					671:680	morphology	671:680	morphology	671:680	The structure and morphology of the prepared adsorbent and fluoride sorbed adsorbent was analysed using FTIR, SEM and EDAX with mapping techniques.
28214583	4	18	theme	adsorbent	698:706	arg1	structure					657:665	structure	657:665	structure	657:665	The structure and morphology of the prepared adsorbent and fluoride sorbed adsorbent was analysed using FTIR, SEM and EDAX with mapping techniques.
28214583	2	19	theme	separation	377:386	arg1	ability					388:394	the good separation ability	368:394	the good separation ability	368:394	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	1	20	theme	magnetic	172:179	arg1	HTCS					232:235	Fe3O4@HTCS	226:235	Fe3O4@HTCS	226:235	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	1	20	theme	magnetic	172:179	arg1	hydrotalcite/chitosan					203:223	magnetic iron oxide fabricated hydrotalcite/chitosan	172:223	magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water	172:309	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	0	21	from	water	38:42	arg1	Remediation					0:10	Remediation	0:10	Remediation of fluoride from drinking water using magnetic iron oxide	0:68	Remediation of fluoride from drinking water using magnetic iron oxide coated hydrotalcite/chitosan composite.
28214583	8	22	theme	adaptable	1269:1277	arg1	nature					1279:1284	its adaptable nature	1265:1284	its adaptable nature	1265:1284	The performance of Fe3O4@HTCS composite to field water sample designates its adaptable nature at field conditions.
28214583	6	23	theme	equilibrium	991:1001	arg1	data					1003:1006	the equilibrium data	987:1006	the equilibrium data	987:1006	From isotherm modeling, the equilibrium data is well described by Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms.
28214583	1	24	theme	iron	181:184	arg1	HTCS					232:235	Fe3O4@HTCS	226:235	Fe3O4@HTCS	226:235	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	1	24	theme	iron	181:184	arg1	hydrotalcite/chitosan					203:223	magnetic iron oxide fabricated hydrotalcite/chitosan	172:223	magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water	172:309	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	8	25	theme	composite	1222:1230	arg1	performance					1196:1206	The performance	1192:1206	The performance of Fe3O4@HTCS composite to field water sample	1192:1252	The performance of Fe3O4@HTCS composite to field water sample designates its adaptable nature at field conditions.
28214583	2	26	theme	developed	316:324	arg1	HTCS					332:335	The developed Fe3O4@HTCS	312:335	The developed Fe3O4@HTCS composite	312:345	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	1	27	theme	oxide	186:190	arg1	HTCS					232:235	Fe3O4@HTCS	226:235	Fe3O4@HTCS	226:235	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	1	27	theme	oxide	186:190	arg1	hydrotalcite/chitosan					203:223	magnetic iron oxide fabricated hydrotalcite/chitosan	172:223	magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water	172:309	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	1	28	theme	fluoride	274:281	arg1	content					283:289	excess fluoride content	267:289	excess fluoride content	267:289	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	3	29	theme	Fe3O4	549:553	arg1	composite					558:566	Fe3O4@HT composite	549:566	Fe3O4@HT composite	549:566	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	29	theme	Fe3O4	549:553	arg1	HTCS					533:536	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	1	30	theme	fabricated	192:201	arg1	HTCS					232:235	Fe3O4@HTCS	226:235	Fe3O4@HTCS	226:235	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	1	30	theme	fabricated	192:201	arg1	hydrotalcite/chitosan					203:223	magnetic iron oxide fabricated hydrotalcite/chitosan	172:223	magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water	172:309	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	2	31	theme	defluoridation	433:446	arg1	capacity					448:455	an extreme enhanced defluoridation capacity	413:455	an extreme enhanced defluoridation capacity (DC)	413:460	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	2	31	theme	defluoridation	433:446	arg1	DC					458:459	DC	458:459	DC	458:459	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	1	32	theme	content	283:289	arg1	removal					256:262	the removal	252:262	the removal of excess fluoride content from drinking water	252:309	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	8	33	theme	HTCS	1217:1220	arg1	composite					1222:1230	Fe3O4@HTCS composite	1211:1230	Fe3O4@HTCS composite	1211:1230	The performance of Fe3O4@HTCS composite to field water sample designates its adaptable nature at field conditions.
28214583	3	34	theme	HTCS	533:536	arg1	5032					602:605	5032	602:605	5032	602:605	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	34	theme	HTCS	533:536	arg1	DCs					520:522	The DCs	516:522	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	516:584	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	1	35	theme	hydrotalcite/chitosan	203:223	arg1	probability					157:167	the probability	153:167	the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water	153:309	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	0	36	theme	fluoride	15:22	arg1	Remediation					0:10	Remediation	0:10	Remediation of fluoride from drinking water using magnetic iron oxide	0:68	Remediation of fluoride from drinking water using magnetic iron oxide coated hydrotalcite/chitosan composite.
28214583	5	37	theme	fluoride	857:864	arg1	concentration					866:878	initial fluoride concentration	849:878	initial fluoride concentration	849:878	The dependence of DC on various parameters like initial fluoride concentration, pH, contact time, interfering anions and temperature was studied by batch method.
28214583	1	38	theme	present	114:120	arg1	study					122:126	The present study	110:126	The present study	110:126	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	4	39	dep	adsorbent	698:706	arg1	adsorbent					728:736	adsorbent	728:736	adsorbent	728:736	The structure and morphology of the prepared adsorbent and fluoride sorbed adsorbent was analysed using FTIR, SEM and EDAX with mapping techniques.
28214583	1	40	theme	drinking	296:303	arg1	water					305:309	drinking water	296:309	drinking water	296:309	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	6	41	theme	Freundlich	1029:1038	arg1	isotherms					1081:1089	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1029:1089	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1029:1089	From isotherm modeling, the equilibrium data is well described by Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms.
28214583	8	42	theme	field	1235:1239	arg1	sample					1247:1252	field water sample	1235:1252	field water sample	1235:1252	The performance of Fe3O4@HTCS composite to field water sample designates its adaptable nature at field conditions.
28214583	2	43	theme	good	372:375	arg1	ability					388:394	the good separation ability	368:394	the good separation ability	368:394	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	1	44	theme	Fe3O4	226:230	arg1	HTCS					232:235	Fe3O4@HTCS	226:235	Fe3O4@HTCS	226:235	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	1	44	theme	Fe3O4	226:230	arg1	hydrotalcite/chitosan					203:223	magnetic iron oxide fabricated hydrotalcite/chitosan	172:223	magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water	172:309	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	0	45	theme	drinking	29:36	arg1	water					38:42	drinking water	29:42	drinking water using magnetic iron oxide	29:68	Remediation of fluoride from drinking water using magnetic iron oxide coated hydrotalcite/chitosan composite.
28214583	8	46	theme	water	1241:1245	arg1	sample					1247:1252	field water sample	1235:1252	field water sample	1235:1252	The performance of Fe3O4@HTCS composite to field water sample designates its adaptable nature at field conditions.
28214583	1	47	theme	@	231:231	arg1	HTCS					232:235	Fe3O4@HTCS	226:235	Fe3O4@HTCS	226:235	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	1	47	theme	@	231:231	arg1	hydrotalcite/chitosan					203:223	magnetic iron oxide fabricated hydrotalcite/chitosan	172:223	magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water	172:309	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	0	48	theme	magnetic	50:57	arg1	oxide					64:68	magnetic iron oxide	50:68	magnetic iron oxide	50:68	Remediation of fluoride from drinking water using magnetic iron oxide coated hydrotalcite/chitosan composite.
28214583	8	49	theme	field	1289:1293	arg1	conditions					1295:1304	field conditions	1289:1304	field conditions	1289:1304	The performance of Fe3O4@HTCS composite to field water sample designates its adaptable nature at field conditions.
28214583	0	50	theme	iron	59:62	arg1	oxide					64:68	magnetic iron oxide	50:68	magnetic iron oxide	50:68	Remediation of fluoride from drinking water using magnetic iron oxide coated hydrotalcite/chitosan composite.
28214583	3	51	theme	@	554:554	arg1	composite					558:566	Fe3O4@HT composite	549:566	Fe3O4@HT composite	549:566	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	51	theme	@	554:554	arg1	HTCS					533:536	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	2	52	theme	composite	337:345	arg1	HTCS					332:335	The developed Fe3O4@HTCS	312:335	The developed Fe3O4@HTCS composite	312:345	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	6	53	theme	isotherm	968:975	arg1	modeling					977:984	isotherm modeling	968:984	isotherm modeling	968:984	From isotherm modeling, the equilibrium data is well described by Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms.
28214583	5	54	theme	batch	949:953	arg1	method					955:960	batch method	949:960	batch method	949:960	The dependence of DC on various parameters like initial fluoride concentration, pH, contact time, interfering anions and temperature was studied by batch method.
28214583	3	55	theme	HT	555:556	arg1	composite					558:566	Fe3O4@HT composite	549:566	Fe3O4@HT composite	549:566	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	55	theme	HT	555:556	arg1	HTCS					533:536	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	5	56	theme	various	825:831	arg1	parameters					833:842	various parameters	825:842	various parameters like initial fluoride concentration, pH, contact time, interfering anions and temperature	825:932	The dependence of DC on various parameters like initial fluoride concentration, pH, contact time, interfering anions and temperature was studied by batch method.
28214583	4	57	theme	fluoride	712:719	arg1	morphology					671:680	morphology	671:680	morphology	671:680	The structure and morphology of the prepared adsorbent and fluoride sorbed adsorbent was analysed using FTIR, SEM and EDAX with mapping techniques.
28214583	4	57	theme	fluoride	712:719	arg1	structure					657:665	structure	657:665	structure	657:665	The structure and morphology of the prepared adsorbent and fluoride sorbed adsorbent was analysed using FTIR, SEM and EDAX with mapping techniques.
28214583	2	58	theme	@	331:331	arg1	HTCS					332:335	The developed Fe3O4@HTCS	312:335	The developed Fe3O4@HTCS composite	312:345	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	4	59	with	FTIR	757:760	arg1	techniques					789:798	mapping techniques	781:798	mapping techniques	781:798	The structure and morphology of the prepared adsorbent and fluoride sorbed adsorbent was analysed using FTIR, SEM and EDAX with mapping techniques.
28214583	5	60	theme	interfering	899:909	arg1	anions					911:916	interfering anions	899:916	interfering anions	899:916	The dependence of DC on various parameters like initial fluoride concentration, pH, contact time, interfering anions and temperature was studied by batch method.
28214583	2	61	theme	Fe3O4	326:330	arg1	HTCS					332:335	The developed Fe3O4@HTCS	312:335	The developed Fe3O4@HTCS composite	312:345	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	4	62	dep	structure	657:665	arg1	The					653:655	The	653:655	The	653:655	The structure and morphology of the prepared adsorbent and fluoride sorbed adsorbent was analysed using FTIR, SEM and EDAX with mapping techniques.
28214583	7	63	theme	endothermic	1145:1155	arg1	nature					1157:1162	the spontaneity and endothermic nature	1125:1162	the spontaneity and endothermic nature of the fluoride adsorption	1125:1189	Thermodynamic parameters confirm the spontaneity and endothermic nature of the fluoride adsorption.
28214583	5	64	theme	contact	885:891	arg1	time					893:896	contact time	885:896	contact time	885:896	The dependence of DC on various parameters like initial fluoride concentration, pH, contact time, interfering anions and temperature was studied by batch method.
28214583	2	65	theme	extreme	416:422	arg1	capacity					448:455	an extreme enhanced defluoridation capacity	413:455	an extreme enhanced defluoridation capacity (DC)	413:460	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	2	65	theme	extreme	416:422	arg1	DC					458:459	DC	458:459	DC	458:459	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	3	66	dep	HTCS	533:536	arg1	composite					558:566	Fe3O4@HT composite	549:566	Fe3O4@HT composite	549:566	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	66	dep	HTCS	533:536	arg1	CS					583:584	CS	583:584	CS	583:584	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	66	dep	HTCS	533:536	arg1	HTCS					533:536	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS	527:584	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	66	dep	HTCS	533:536	arg1	composite					538:546	composite	538:546	composite	538:546	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	66	dep	HTCS	533:536	arg1	Fe3O4					569:573	Fe3O4	569:573	Fe3O4	569:573	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	3	66	dep	HTCS	533:536	arg1	HT					576:577	HT	576:577	HT	576:577	The DCs of Fe3O4@HTCS composite, Fe3O4@HT composite, Fe3O4, HT and CS was found to be 5032, 3041, 1050, 1030 and 52mgF-/kg respectively.
28214583	1	67	theme	composite	238:246	arg1	HTCS					232:235	Fe3O4@HTCS	226:235	Fe3O4@HTCS	226:235	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	1	67	theme	composite	238:246	arg1	hydrotalcite/chitosan					203:223	magnetic iron oxide fabricated hydrotalcite/chitosan	172:223	magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water	172:309	The present study was performed to examine the probability of magnetic iron oxide fabricated hydrotalcite/chitosan (Fe3O4@HTCS) composite for the removal of excess fluoride content from drinking water.
28214583	2	68	theme	base	485:488	arg1	components					490:499	other base components	479:499	other base components	479:499	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	2	69	theme	enhanced	424:431	arg1	capacity					448:455	an extreme enhanced defluoridation capacity	413:455	an extreme enhanced defluoridation capacity (DC)	413:460	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	2	69	theme	enhanced	424:431	arg1	DC					458:459	DC	458:459	DC	458:459	The developed Fe3O4@HTCS composite not only demonstrate the good separation ability but also display an extreme enhanced defluoridation capacity (DC) when compared to other base components and composite.
28214583	7	70	theme	adsorption	1180:1189	arg1	nature					1157:1162	the spontaneity and endothermic nature	1125:1162	the spontaneity and endothermic nature of the fluoride adsorption	1125:1189	Thermodynamic parameters confirm the spontaneity and endothermic nature of the fluoride adsorption.
28214583	4	71	with	EDAX	771:774	arg1	techniques					789:798	mapping techniques	781:798	mapping techniques	781:798	The structure and morphology of the prepared adsorbent and fluoride sorbed adsorbent was analysed using FTIR, SEM and EDAX with mapping techniques.
28429501	7	0	theme	products	1609:1616	arg1	application					1581:1591	the high-value application	1566:1591	the high-value application of wheat gluten products	1566:1616	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	6	1	theme	irregular	1189:1197	arg1	structure					1221:1229	the irregular wheat gluten granular structure	1185:1229	the irregular wheat gluten granular structure	1185:1229	Microstructures suggested that the irregular wheat gluten granular structure was disrupted, with additive addition favoring transformation into a more layered or fibrous structure during twin-screw extrusion.
28429501	7	2	theme	wheat	1596:1600	arg1	products					1609:1616	wheat gluten products	1596:1616	wheat gluten products	1596:1616	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	2	3	from	effect	319:324	arg1	properties					387:396	the physicochemical and structural properties	352:396	the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion	352:456	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	4	4	dep	in	763:764	arg1	vitro					766:770	vitro	766:770	vitro	766:770	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	2	5	theme	wheat	280:284	arg1	gluten					286:291	wheat gluten	280:291	wheat gluten	280:291	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	1	6	theme	vegetable	185:193	arg1	protein					195:201	inexpensive vegetable protein	173:201	inexpensive vegetable protein with an ideal amino acid composition	173:238	BACKGROUND Wheat gluten comprises a good quality and inexpensive vegetable protein with an ideal amino acid composition.
28429501	4	7	theme	sulfhydryl	911:920	arg1	content					933:939	free sulfhydryl group (SH) content	906:939	free sulfhydryl group (SH) content	906:939	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	2	8	theme	food	300:303	arg1	industry					305:312	the food industry	296:312	the food industry	296:312	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	1	9	theme	ideal	211:215	arg1	composition					228:238	an ideal amino acid composition	208:238	an ideal amino acid composition	208:238	BACKGROUND Wheat gluten comprises a good quality and inexpensive vegetable protein with an ideal amino acid composition.
28429501	3	10	theme	disulfide	630:638	arg1	bonds					640:644	new disulfide bonds	626:644	new disulfide bonds	626:644	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
28429501	1	11	theme	acid	223:226	arg1	composition					228:238	an ideal amino acid composition	208:238	an ideal amino acid composition	208:238	BACKGROUND Wheat gluten comprises a good quality and inexpensive vegetable protein with an ideal amino acid composition.
28429501	7	12	theme	gluten-based	1490:1501	arg1	foods					1503:1507	extruded wheat gluten-based foods	1475:1507	extruded wheat gluten-based foods	1475:1507	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	1	13	theme	Wheat	131:135	arg1	gluten					137:142	BACKGROUND Wheat gluten	120:142	BACKGROUND Wheat gluten	120:142	BACKGROUND Wheat gluten comprises a good quality and inexpensive vegetable protein with an ideal amino acid composition.
28429501	6	14	theme	fibrous	1316:1322	arg1	structure					1324:1332	a more layered or fibrous structure	1298:1332	a more layered or fibrous structure	1298:1332	Microstructures suggested that the irregular wheat gluten granular structure was disrupted, with additive addition favoring transformation into a more layered or fibrous structure during twin-screw extrusion.
28429501	7	15	theme	extruded	1475:1482	arg1	foods					1503:1507	extruded wheat gluten-based foods	1475:1507	extruded wheat gluten-based foods	1475:1507	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	4	16	theme	in	763:764	arg1	digestibility					780:792	in vitro protein digestibility	763:792	in vitro protein digestibility	763:792	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	5	17	theme	protein	1133:1139	arg1	aggregation					1141:1151	protein aggregation	1133:1151	protein aggregation	1133:1151	Secondary structural analysis showed that α-helices disappeared with the concomitant increase of antiparallel β-sheets, demonstrating the occurrence of protein aggregation.
28429501	3	18	theme	interactions	663:674	arg1	formation					613:621	the formation	609:621	the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products	609:713	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
28429501	5	19	theme	Secondary	981:989	arg1	analysis					1002:1009	Secondary structural analysis	981:1009	Secondary structural analysis	981:1009	Secondary structural analysis showed that α-helices disappeared with the concomitant increase of antiparallel β-sheets, demonstrating the occurrence of protein aggregation.
28429501	6	20	theme	layered	1305:1311	arg1	structure					1324:1332	a more layered or fibrous structure	1298:1332	a more layered or fibrous structure	1298:1332	Microstructures suggested that the irregular wheat gluten granular structure was disrupted, with additive addition favoring transformation into a more layered or fibrous structure during twin-screw extrusion.
28429501	0	21	theme	twin-screw	67:76	arg1	extrusion					78:86	twin-screw extrusion	67:86	twin-screw extrusion	67:86	Formation of macromolecules in wheat gluten/starch mixtures during twin-screw extrusion: effect of different additives.
28429501	1	22	theme	good	156:159	arg1	quality					161:167	a good quality	154:167	a good quality	154:167	BACKGROUND Wheat gluten comprises a good quality and inexpensive vegetable protein with an ideal amino acid composition.
28429501	4	23	theme	water	734:738	arg1	capacity					750:757	water retention capacity	734:757	water retention capacity	734:757	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	0	24	from	Formation	0:8	arg1	gluten/starch					37:49	wheat gluten/starch	31:49	wheat gluten/starch	31:49	Formation of macromolecules in wheat gluten/starch mixtures during twin-screw extrusion: effect of different additives.
28429501	4	25	theme	extruded	801:808	arg1	products					830:837	all extruded wheat gluten/starch products	797:837	all extruded wheat gluten/starch products	797:837	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	4	26	theme	gluten/starch	816:828	arg1	products					830:837	all extruded wheat gluten/starch products	797:837	all extruded wheat gluten/starch products	797:837	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	2	27	theme	wheat	401:405	arg1	mixtures					421:428	wheat gluten/starch mixtures	401:428	wheat gluten/starch mixtures during twin-screw extrusion	401:456	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	0	28	theme	additives	109:117	arg1	effect					89:94	effect	89:94	effect of different additives	89:117	Formation of macromolecules in wheat gluten/starch mixtures during twin-screw extrusion: effect of different additives.
28429501	4	29	theme	protein	772:778	arg1	digestibility					780:792	in vitro protein digestibility	763:792	in vitro protein digestibility	763:792	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	7	30	dep	CONCLUSION	1363:1372	arg1	demonstrate					1408:1418	demonstrate	1408:1418	demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods	1408:1507	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	7	30	dep	CONCLUSION	1363:1372	arg1	suggest					1513:1519	suggest	1513:1519	suggest that this process might serve as a basis for the high-value application of wheat gluten products	1513:1616	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	2	31	theme	potential	255:263	arg1	application					265:275	the potential application	251:275	the potential application of wheat gluten	251:291	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	2	32	theme	twin-screw	437:446	arg1	extrusion					448:456	twin-screw extrusion	437:456	twin-screw extrusion	437:456	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	0	33	theme	macromolecules	13:26	arg1	Formation					0:8	Formation	0:8	Formation of macromolecules in wheat gluten/starch	0:49	Formation of macromolecules in wheat gluten/starch mixtures during twin-screw extrusion: effect of different additives.
28429501	2	34	theme	mixtures	421:428	arg1	properties					387:396	the physicochemical and structural properties	352:396	the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion	352:456	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	3	35	theme	RESULTS	476:482	arg1	Macromolecules					484:497	RESULTS Macromolecules	476:497	RESULTS Macromolecules	476:497	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
28429501	0	36	theme	wheat	31:35	arg1	gluten/starch					37:49	wheat gluten/starch	31:49	wheat gluten/starch	31:49	Formation of macromolecules in wheat gluten/starch mixtures during twin-screw extrusion: effect of different additives.
28429501	0	37	dep	mixtures	51:58	arg1	effect					89:94	effect	89:94	effect of different additives	89:117	Formation of macromolecules in wheat gluten/starch mixtures during twin-screw extrusion: effect of different additives.
28429501	4	38	theme	solubility	885:894	arg1	index					896:900	the nitrogen solubility index	872:900	the nitrogen solubility index	872:900	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	2	39	theme	additives	339:347	arg1	effect					319:324	the effect	315:324	the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion	315:456	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	7	40	theme	study	1402:1406	arg1	findings					1378:1385	The findings	1374:1385	The findings of the present study	1374:1406	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	6	41	theme	granular	1212:1219	arg1	structure					1221:1229	the irregular wheat gluten granular structure	1185:1229	the irregular wheat gluten granular structure	1185:1229	Microstructures suggested that the irregular wheat gluten granular structure was disrupted, with additive addition favoring transformation into a more layered or fibrous structure during twin-screw extrusion.
28429501	3	42	theme	reaction	697:704	arg1	products					706:713	Maillard reaction products	688:713	Maillard reaction products	688:713	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
28429501	4	43	dep	increased	853:861	arg1	whereas					864:870	whereas	864:870	whereas	864:870	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	7	44	theme	gluten	1602:1607	arg1	products					1609:1616	wheat gluten products	1596:1616	wheat gluten products	1596:1616	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	5	45	theme	antiparallel	1078:1089	arg1	β-sheets					1091:1098	antiparallel β-sheets	1078:1098	antiparallel β-sheets	1078:1098	Secondary structural analysis showed that α-helices disappeared with the concomitant increase of antiparallel β-sheets, demonstrating the occurrence of protein aggregation.
28429501	6	46	theme	wheat	1199:1203	arg1	structure					1221:1229	the irregular wheat gluten granular structure	1185:1229	the irregular wheat gluten granular structure	1185:1229	Microstructures suggested that the irregular wheat gluten granular structure was disrupted, with additive addition favoring transformation into a more layered or fibrous structure during twin-screw extrusion.
28429501	3	47	theme	gluten/starch	530:542	arg1	mixtures					544:551	wheat gluten/starch mixtures	524:551	wheat gluten/starch mixtures	524:551	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
28429501	4	48	theme	group	922:926	arg1	content					933:939	free sulfhydryl group (SH) content	906:939	free sulfhydryl group (SH) content	906:939	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	7	49	theme	high-value	1570:1579	arg1	application					1581:1591	the high-value application	1566:1591	the high-value application of wheat gluten products	1566:1616	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	4	50	theme	SH	929:930	arg1	content					933:939	free sulfhydryl group (SH) content	906:939	free sulfhydryl group (SH) content	906:939	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	2	51	theme	gluten	286:291	arg1	application					265:275	the potential application	251:275	the potential application of wheat gluten	251:291	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	1	52	theme	inexpensive	173:183	arg1	protein					195:201	inexpensive vegetable protein	173:201	inexpensive vegetable protein with an ideal amino acid composition	173:238	BACKGROUND Wheat gluten comprises a good quality and inexpensive vegetable protein with an ideal amino acid composition.
28429501	4	53	theme	free	906:909	arg1	content					933:939	free sulfhydryl group (SH) content	906:939	free sulfhydryl group (SH) content	906:939	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	6	54	theme	additive	1251:1258	arg1	addition					1260:1267	additive addition	1251:1267	additive addition favoring transformation into a more layered or fibrous structure during twin-screw extrusion	1251:1360	Microstructures suggested that the irregular wheat gluten granular structure was disrupted, with additive addition favoring transformation into a more layered or fibrous structure during twin-screw extrusion.
28429501	4	55	theme	twin-screw	959:968	arg1	extrusion					970:978	twin-screw extrusion	959:978	twin-screw extrusion	959:978	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	3	56	theme	wheat	524:528	arg1	mixtures					544:551	wheat gluten/starch mixtures	524:551	wheat gluten/starch mixtures	524:551	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
28429501	3	57	theme	new	626:628	arg1	bonds					640:644	new disulfide bonds	626:644	new disulfide bonds	626:644	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
28429501	1	58	theme	amino	217:221	arg1	composition					228:238	an ideal amino acid composition	208:238	an ideal amino acid composition	208:238	BACKGROUND Wheat gluten comprises a good quality and inexpensive vegetable protein with an ideal amino acid composition.
28429501	6	59	theme	twin-screw	1341:1350	arg1	extrusion					1352:1360	twin-screw extrusion	1341:1360	twin-screw extrusion	1341:1360	Microstructures suggested that the irregular wheat gluten granular structure was disrupted, with additive addition favoring transformation into a more layered or fibrous structure during twin-screw extrusion.
28429501	1	60	theme	BACKGROUND	120:129	arg1	gluten					137:142	BACKGROUND Wheat gluten	120:142	BACKGROUND Wheat gluten	120:142	BACKGROUND Wheat gluten comprises a good quality and inexpensive vegetable protein with an ideal amino acid composition.
28429501	7	61	theme	foods	1503:1507	arg1	quality					1464:1470	quality	1464:1470	quality	1464:1470	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	7	61	theme	foods	1503:1507	arg1	texture					1452:1458	texture	1452:1458	texture	1452:1458	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	4	62	theme	retention	740:748	arg1	capacity					750:757	water retention capacity	734:757	water retention capacity	734:757	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	3	63	theme	non-covalent	650:661	arg1	interactions					663:674	non-covalent interactions	650:674	non-covalent interactions	650:674	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
28429501	7	64	theme	wheat	1484:1488	arg1	foods					1503:1507	extruded wheat gluten-based foods	1475:1507	extruded wheat gluten-based foods	1475:1507	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	5	65	theme	aggregation	1141:1151	arg1	occurrence					1119:1128	the occurrence	1115:1128	the occurrence of protein aggregation	1115:1151	Secondary structural analysis showed that α-helices disappeared with the concomitant increase of antiparallel β-sheets, demonstrating the occurrence of protein aggregation.
28429501	3	66	theme	bonds	640:644	arg1	formation					613:621	the formation	609:621	the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products	609:713	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
28429501	1	67	with	quality	161:167	arg1	composition					228:238	an ideal amino acid composition	208:238	an ideal amino acid composition	208:238	BACKGROUND Wheat gluten comprises a good quality and inexpensive vegetable protein with an ideal amino acid composition.
28429501	0	68	theme	different	99:107	arg1	additives					109:117	different additives	99:117	different additives	99:117	Formation of macromolecules in wheat gluten/starch mixtures during twin-screw extrusion: effect of different additives.
28429501	8	69	theme	Chemical	1637:1644	arg1	Industry					1646:1653	Chemical Industry	1637:1653	Chemical Industry	1637:1653	© 2017 Society of Chemical Industry.
28429501	4	70	theme	wheat	810:814	arg1	products					830:837	all extruded wheat gluten/starch products	797:837	all extruded wheat gluten/starch products	797:837	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	5	71	theme	structural	991:1000	arg1	analysis					1002:1009	Secondary structural analysis	981:1009	Secondary structural analysis	981:1009	Secondary structural analysis showed that α-helices disappeared with the concomitant increase of antiparallel β-sheets, demonstrating the occurrence of protein aggregation.
28429501	2	72	theme	structural	376:385	arg1	properties					387:396	the physicochemical and structural properties	352:396	the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion	352:456	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	4	73	dep	capacity	750:757	arg1	the					730:732	the	730:732	the	730:732	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	5	74	theme	β-sheets	1091:1098	arg1	increase					1066:1073	the concomitant increase	1050:1073	the concomitant increase of antiparallel β-sheets	1050:1098	Secondary structural analysis showed that α-helices disappeared with the concomitant increase of antiparallel β-sheets, demonstrating the occurrence of protein aggregation.
28429501	2	75	theme	gluten/starch	407:419	arg1	mixtures					421:428	wheat gluten/starch mixtures	401:428	wheat gluten/starch mixtures during twin-screw extrusion	401:456	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	1	76	with	protein	195:201	arg1	composition					228:238	an ideal amino acid composition	208:238	an ideal amino acid composition	208:238	BACKGROUND Wheat gluten comprises a good quality and inexpensive vegetable protein with an ideal amino acid composition.
28429501	7	77	dep	texture	1452:1458	arg1	the					1448:1450	the	1448:1450	the	1448:1450	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	7	78	theme	present	1394:1400	arg1	study					1402:1406	the present study	1390:1406	the present study	1390:1406	CONCLUSION The findings of the present study demonstrate that extrusion might affect the texture and quality of extruded wheat gluten-based foods and suggest that this process might serve as a basis for the high-value application of wheat gluten products.
28429501	3	79	theme	products	706:713	arg1	formation					613:621	the formation	609:621	the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products	609:713	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
28429501	4	80	theme	nitrogen	876:883	arg1	index					896:900	the nitrogen solubility index	872:900	the nitrogen solubility index	872:900	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	2	81	theme	different	329:337	arg1	additives					339:347	different additives	329:347	different additives	329:347	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	5	82	theme	concomitant	1054:1064	arg1	increase					1066:1073	the concomitant increase	1050:1073	the concomitant increase of antiparallel β-sheets	1050:1098	Secondary structural analysis showed that α-helices disappeared with the concomitant increase of antiparallel β-sheets, demonstrating the occurrence of protein aggregation.
28429501	3	83	theme	Maillard	688:695	arg1	products					706:713	Maillard reaction products	688:713	Maillard reaction products	688:713	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
28429501	4	84	theme	products	830:837	arg1	digestibility					780:792	in vitro protein digestibility	763:792	in vitro protein digestibility	763:792	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	4	84	theme	products	830:837	arg1	capacity					750:757	water retention capacity	734:757	water retention capacity	734:757	Additionally, the water retention capacity and in vitro protein digestibility of all extruded wheat gluten/starch products significantly increased, whereas the nitrogen solubility index and free sulfhydryl group (SH) content decreased, during twin-screw extrusion.
28429501	2	85	theme	physicochemical	356:370	arg1	properties					387:396	the physicochemical and structural properties	352:396	the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion	352:456	To expand the potential application of wheat gluten in the food industry, the effect of different additives on the physicochemical and structural properties of wheat gluten/starch mixtures during twin-screw extrusion was investigated.
28429501	6	86	theme	gluten	1205:1210	arg1	structure					1221:1229	the irregular wheat gluten granular structure	1185:1229	the irregular wheat gluten granular structure	1185:1229	Microstructures suggested that the irregular wheat gluten granular structure was disrupted, with additive addition favoring transformation into a more layered or fibrous structure during twin-screw extrusion.
28429501	3	87	theme	twin-screw	560:569	arg1	extrusion					571:579	twin-screw extrusion	560:579	twin-screw extrusion	560:579	RESULTS Macromolecules were observed to form in wheat gluten/starch mixtures during twin-screw extrusion, which may be attributed to the formation of new disulfide bonds and non-covalent interactions, as well as Maillard reaction products.
24401457	4	0	dep	resolutions	874:884	arg1	compared					889:896	compared	889:896	compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5)	889:981	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	6	1	theme	RSD	1276:1278	arg1	variations					1280:1289	Typical RSD variations	1268:1289	Typical RSD variations for 6 successive experiments	1268:1318	Typical RSD variations for 6 successive experiments were between 0.8% and 1.7% for peak migration times and between 1.2% and 4.9% for normalized corrected peak areas.
24401457	5	2	theme	sample	1143:1148	arg1	compositions					1150:1161	appropriate electrolyte and sample compositions	1115:1161	compositions	1150:1161	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	0	3	theme	capillary	97:105	arg1	electrophoresis					107:121	cyclodextrin-modified capillary electrophoresis	75:121	cyclodextrin-modified capillary electrophoresis	75:121	Optimizing separation conditions of 19 polycyclic aromatic hydrocarbons by cyclodextrin-modified capillary electrophoresis and applications to edible oils.
24401457	6	4	theme	Typical	1268:1274	arg1	variations					1280:1289	Typical RSD variations	1268:1289	Typical RSD variations for 6 successive experiments	1268:1318	Typical RSD variations for 6 successive experiments were between 0.8% and 1.7% for peak migration times and between 1.2% and 4.9% for normalized corrected peak areas.
24401457	5	5	with	use	1066:1068	arg1	material					1196:1203	sample vial material	1184:1203	sample vial material	1184:1203	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	5	5	with	use	1066:1068	arg1	electrolyte					1127:1137	appropriate electrolyte and sample compositions	1115:1161	electrolyte	1127:1137	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	5	5	with	use	1066:1068	arg1	compositions					1150:1161	appropriate electrolyte and sample compositions	1115:1161	compositions	1150:1161	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	5	5	with	use	1066:1068	arg1	sequences					1170:1178	rinse sequences	1164:1178	rinse sequences	1164:1178	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	8	6	theme	real	1542:1545	arg1	extracts					1561:1568	real vegetable oil extracts	1542:1568	real vegetable oil extracts	1542:1568	For the first time in capillary electrophoresis, applications to real vegetable oil extracts were successfully carried out using the separation method developed here.
24401457	1	7	theme	-modified	436:444	arg1	electrophoresis					461:475	cyclodextrin (CD)-modified capillary zone electrophoresis	419:475	cyclodextrin (CD)-modified capillary zone electrophoresis	419:475	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	8	8	theme	first	1485:1489	arg1	time					1491:1494	the first time	1481:1494	the first time in capillary electrophoresis	1481:1523	For the first time in capillary electrophoresis, applications to real vegetable oil extracts were successfully carried out using the separation method developed here.
24401457	8	9	theme	separation	1610:1619	arg1	method					1621:1626	the separation method	1606:1626	the separation method developed here	1606:1641	For the first time in capillary electrophoresis, applications to real vegetable oil extracts were successfully carried out using the separation method developed here.
24401457	1	10	theme	environmental	270:282	arg1	samples					293:299	environmental and food samples	270:299	environmental and food samples	270:299	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	4	11	theme	PAHs	855:858	arg1	Separation					838:847	Separation	838:847	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5)	838:981	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	1	12	theme	capillary	446:454	arg1	electrophoresis					461:475	cyclodextrin (CD)-modified capillary zone electrophoresis	419:475	cyclodextrin (CD)-modified capillary zone electrophoresis	419:475	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	5	13	theme	rinse	1164:1168	arg1	sequences					1170:1178	rinse sequences	1164:1178	rinse sequences	1164:1178	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	0	14	theme	edible	143:148	arg1	oils					150:153	edible oils	143:153	edible oils	143:153	Optimizing separation conditions of 19 polycyclic aromatic hydrocarbons by cyclodextrin-modified capillary electrophoresis and applications to edible oils.
24401457	2	15	theme	CD	616:617	arg1	mixture					593:599	a mixture	591:599	a mixture of one neutral CD and one anionic CD	591:636	The use of a dual CD system, involving a mixture of one neutral CD and one anionic CD, enabled to reach unique selectivity.
24401457	1	16	theme	zone	456:459	arg1	electrophoresis					461:475	cyclodextrin (CD)-modified capillary zone electrophoresis	419:475	cyclodextrin (CD)-modified capillary zone electrophoresis	419:475	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	1	17	dep	wavelength	531:540	arg1	nm					547:548	325 nm	543:548	325 nm	543:548	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	4	18	theme	US-EPA	930:935	arg1	PAHs					937:940	the 16 US-EPA PAHs	923:940	the 16 US-EPA PAHs	923:940	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	2	19	theme	neutral	608:614	arg1	CD					616:617	CD	616:617	one neutral CD	604:617	The use of a dual CD system, involving a mixture of one neutral CD and one anionic CD, enabled to reach unique selectivity.
24401457	6	20	theme	successive	1297:1306	arg1	experiments					1308:1318	6 successive experiments	1295:1318	6 successive experiments	1295:1318	Typical RSD variations for 6 successive experiments were between 0.8% and 1.7% for peak migration times and between 1.2% and 4.9% for normalized corrected peak areas.
24401457	8	21	theme	capillary	1499:1507	arg1	electrophoresis					1509:1523	capillary electrophoresis	1499:1523	capillary electrophoresis	1499:1523	For the first time in capillary electrophoresis, applications to real vegetable oil extracts were successfully carried out using the separation method developed here.
24401457	0	22	theme	separation	11:20	arg1	conditions					22:31	separation conditions	11:31	separation conditions of 19 polycyclic aromatic hydrocarbons	11:70	Optimizing separation conditions of 19 polycyclic aromatic hydrocarbons by cyclodextrin-modified capillary electrophoresis and applications to edible oils.
24401457	1	23	dep	States	315:320	arg1	US-EPA					355:360	US-EPA	355:360	US-EPA	355:360	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	1	23	dep	States	315:320	arg1	Agency					347:352	Environmental Protection Agency	322:352	the United States Environmental Protection Agency (US-EPA)	304:361	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	8	24	from	time	1491:1494	arg1	electrophoresis					1509:1523	capillary electrophoresis	1499:1523	capillary electrophoresis	1499:1523	For the first time in capillary electrophoresis, applications to real vegetable oil extracts were successfully carried out using the separation method developed here.
24401457	3	25	theme	differential	717:728	arg1	partitioning					730:741	their differential partitioning	711:741	their differential partitioning between the two CDs	711:761	As solutes were separated based on their differential partitioning between the two CDs, the CD relative concentrations were investigated to optimize selectivity.
24401457	4	26	theme	enhanced	865:872	arg1	resolutions					874:884	enhanced resolutions	865:884	enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5)	865:981	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	7	27	from	LOQs	1435:1438	arg1	range					1456:1460	the low µg/L range	1443:1460	the low µg/L range	1443:1460	LOQs in the low µg/L range were obtained.
24401457	1	28	theme	food	288:291	arg1	samples					293:299	environmental and food samples	270:299	environmental and food samples	270:299	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	1	29	theme	laser-induced	482:494	arg1	detection					509:517	laser-induced fluorescence detection	482:517	laser-induced fluorescence detection (excitation wavelength: 325 nm)	482:549	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	1	29	theme	laser-induced	482:494	arg1	wavelength					531:540	excitation wavelength	520:540	excitation wavelength	520:540	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	1	30	theme	polycyclic	197:206	arg1	hydrocarbons					217:228	19 polycyclic aromatic hydrocarbons	194:228	19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority	194:400	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	1	30	theme	polycyclic	197:206	arg1	PAHs					231:234	PAHs	231:234	PAHs	231:234	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	2	31	dep	CD	616:617	arg1	CD					635:636	CD	635:636	CD	635:636	The use of a dual CD system, involving a mixture of one neutral CD and one anionic CD, enabled to reach unique selectivity.
24401457	5	32	theme	appropriate	1115:1125	arg1	electrolyte					1127:1137	appropriate electrolyte and sample compositions	1115:1161	electrolyte	1127:1137	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	4	33	theme	previous	903:910	arg1	studies					912:918	previous studies	903:918	previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5)	903:981	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	1	34	theme	fluorescence	496:507	arg1	detection					509:517	laser-induced fluorescence detection	482:517	laser-induced fluorescence detection (excitation wavelength: 325 nm)	482:549	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	1	34	theme	fluorescence	496:507	arg1	wavelength					531:540	excitation wavelength	520:540	excitation wavelength	520:540	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	4	35	theme	20mM	1044:1047	arg1	methyl-β-CD					1049:1059	20mM methyl-β-CD	1044:1059	20mM methyl-β-CD	1044:1059	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	1	36	theme	aromatic	208:215	arg1	hydrocarbons					217:228	19 polycyclic aromatic hydrocarbons	194:228	19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority	194:400	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	1	36	theme	aromatic	208:215	arg1	PAHs					231:234	PAHs	231:234	PAHs	231:234	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	4	37	theme	superior	959:966	arg1	PAHs					937:940	the 16 US-EPA PAHs	923:940	the 16 US-EPA PAHs	923:940	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	5	38	from	improvement	1231:1241	arg1	repeatability					1253:1265	method repeatability	1246:1265	method repeatability	1246:1265	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	1	39	theme	hydrocarbons	217:228	arg1	separation					180:189	the separation	176:189	the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority	176:400	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	0	40	theme	polycyclic	39:48	arg1	hydrocarbons					59:70	19 polycyclic aromatic hydrocarbons	36:70	19 polycyclic aromatic hydrocarbons	36:70	Optimizing separation conditions of 19 polycyclic aromatic hydrocarbons by cyclodextrin-modified capillary electrophoresis and applications to edible oils.
24401457	4	41	from	studies	912:918	arg1	efficiencies					946:957	efficiencies	946:957	efficiencies	946:957	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	4	41	from	studies	912:918	arg1	PAHs					937:940	the 16 US-EPA PAHs	923:940	the 16 US-EPA PAHs	923:940	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	8	42	theme	vegetable	1547:1555	arg1	extracts					1561:1568	real vegetable oil extracts	1542:1568	real vegetable oil extracts	1542:1568	For the first time in capillary electrophoresis, applications to real vegetable oil extracts were successfully carried out using the separation method developed here.
24401457	6	43	theme	normalized	1402:1411	arg1	areas					1428:1432	normalized corrected peak areas	1402:1432	normalized corrected peak areas	1402:1432	Typical RSD variations for 6 successive experiments were between 0.8% and 1.7% for peak migration times and between 1.2% and 4.9% for normalized corrected peak areas.
24401457	5	44	theme	significant	1219:1229	arg1	improvement					1231:1241	a significant improvement	1217:1241	a significant improvement in method repeatability	1217:1265	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	1	45	theme	United	308:313	arg1	States					315:320	the United States Environmental Protection Agency (US-EPA)	304:361	the United States Environmental Protection Agency (US-EPA)	304:361	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	6	46	theme	migration	1356:1364	arg1	times					1366:1370	peak migration times	1351:1370	peak migration times	1351:1370	Typical RSD variations for 6 successive experiments were between 0.8% and 1.7% for peak migration times and between 1.2% and 4.9% for normalized corrected peak areas.
24401457	7	47	theme	low	1447:1449	arg1	range					1456:1460	the low µg/L range	1443:1460	the low µg/L range	1443:1460	LOQs in the low µg/L range were obtained.
24401457	0	48	theme	hydrocarbons	59:70	arg1	conditions					22:31	separation conditions	11:31	separation conditions of 19 polycyclic aromatic hydrocarbons	11:70	Optimizing separation conditions of 19 polycyclic aromatic hydrocarbons by cyclodextrin-modified capillary electrophoresis and applications to edible oils.
24401457	5	49	theme	standard	1085:1092	arg1	use					1066:1068	The use	1062:1068	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material	1062:1203	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	2	50	theme	system	573:578	arg1	use					556:558	The use	552:558	The use of a dual CD system, involving a mixture of one neutral CD and one anionic CD,	552:637	The use of a dual CD system, involving a mixture of one neutral CD and one anionic CD, enabled to reach unique selectivity.
24401457	7	51	theme	µg/L	1451:1454	arg1	range					1456:1460	the low µg/L range	1443:1460	the low µg/L range	1443:1460	LOQs in the low µg/L range were obtained.
24401457	0	52	theme	aromatic	50:57	arg1	hydrocarbons					59:70	19 polycyclic aromatic hydrocarbons	36:70	19 polycyclic aromatic hydrocarbons	36:70	Optimizing separation conditions of 19 polycyclic aromatic hydrocarbons by cyclodextrin-modified capillary electrophoresis and applications to edible oils.
24401457	1	53	theme	Environmental	322:334	arg1	US-EPA					355:360	US-EPA	355:360	US-EPA	355:360	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	1	53	theme	Environmental	322:334	arg1	Agency					347:352	Environmental Protection Agency	322:352	the United States Environmental Protection Agency (US-EPA)	304:361	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	2	54	theme	CD	570:571	arg1	system					573:578	a dual CD system	563:578	a dual CD system	563:578	The use of a dual CD system, involving a mixture of one neutral CD and one anionic CD, enabled to reach unique selectivity.
24401457	1	55	theme	excitation	520:529	arg1	detection					509:517	laser-induced fluorescence detection	482:517	laser-induced fluorescence detection (excitation wavelength: 325 nm)	482:549	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	1	55	theme	excitation	520:529	arg1	wavelength					531:540	excitation wavelength	520:540	excitation wavelength	520:540	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	4	56	theme	sulfobutyl	1018:1027	arg1	ether-β-CD					1029:1038	10mM sulfobutyl ether-β-CD	1013:1038	10mM sulfobutyl ether-β-CD	1013:1038	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	1	57	theme	Protection	336:345	arg1	US-EPA					355:360	US-EPA	355:360	US-EPA	355:360	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	1	57	theme	Protection	336:345	arg1	Agency					347:352	Environmental Protection Agency	322:352	the United States Environmental Protection Agency (US-EPA)	304:361	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	2	58	theme	dual	565:568	arg1	system					573:578	a dual CD system	563:578	a dual CD system	563:578	The use of a dual CD system, involving a mixture of one neutral CD and one anionic CD, enabled to reach unique selectivity.
24401457	6	59	theme	peak	1423:1426	arg1	areas					1428:1432	normalized corrected peak areas	1402:1432	normalized corrected peak areas	1402:1432	Typical RSD variations for 6 successive experiments were between 0.8% and 1.7% for peak migration times and between 1.2% and 4.9% for normalized corrected peak areas.
24401457	2	60	theme	anionic	627:633	arg1	mixture					593:599	a mixture	591:599	a mixture of one neutral CD and one anionic CD	591:636	The use of a dual CD system, involving a mixture of one neutral CD and one anionic CD, enabled to reach unique selectivity.
24401457	8	61	theme	oil	1557:1559	arg1	extracts					1561:1568	real vegetable oil extracts	1542:1568	real vegetable oil extracts	1542:1568	For the first time in capillary electrophoresis, applications to real vegetable oil extracts were successfully carried out using the separation method developed here.
24401457	4	62	theme	10mM	1013:1016	arg1	ether-β-CD					1029:1038	10mM sulfobutyl ether-β-CD	1013:1038	10mM sulfobutyl ether-β-CD	1013:1038	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	3	63	theme	CD	768:769	arg1	concentrations					780:793	the CD relative concentrations	764:793	the CD relative concentrations	764:793	As solutes were separated based on their differential partitioning between the two CDs, the CD relative concentrations were investigated to optimize selectivity.
24401457	2	64	theme	unique	656:661	arg1	selectivity					663:673	unique selectivity	656:673	unique selectivity	656:673	The use of a dual CD system, involving a mixture of one neutral CD and one anionic CD, enabled to reach unique selectivity.
24401457	6	65	theme	corrected	1413:1421	arg1	areas					1428:1432	normalized corrected peak areas	1402:1432	normalized corrected peak areas	1402:1432	Typical RSD variations for 6 successive experiments were between 0.8% and 1.7% for peak migration times and between 1.2% and 4.9% for normalized corrected peak areas.
24401457	3	66	theme	relative	771:778	arg1	concentrations					780:793	the CD relative concentrations	764:793	the CD relative concentrations	764:793	As solutes were separated based on their differential partitioning between the two CDs, the CD relative concentrations were investigated to optimize selectivity.
24401457	0	67	theme	cyclodextrin-modified	75:95	arg1	electrophoresis					107:121	cyclodextrin-modified capillary electrophoresis	75:121	cyclodextrin-modified capillary electrophoresis	75:121	Optimizing separation conditions of 19 polycyclic aromatic hydrocarbons by cyclodextrin-modified capillary electrophoresis and applications to edible oils.
24401457	1	68	theme	first	164:168	arg1	time					170:173	the first time	160:173	the first time	160:173	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
24401457	5	69	theme	method	1246:1251	arg1	repeatability					1253:1265	method repeatability	1246:1265	method repeatability	1246:1265	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	5	70	theme	sample	1184:1189	arg1	material					1196:1203	sample vial material	1184:1203	sample vial material	1184:1203	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	6	71	theme	peak	1351:1354	arg1	migration					1356:1364	peak migration	1351:1364	peak migration times	1351:1370	Typical RSD variations for 6 successive experiments were between 0.8% and 1.7% for peak migration times and between 1.2% and 4.9% for normalized corrected peak areas.
24401457	5	72	theme	vial	1191:1194	arg1	material					1196:1203	sample vial material	1184:1203	sample vial material	1184:1203	The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability.
24401457	4	73	with	Separation	838:847	arg1	resolutions					874:884	enhanced resolutions	865:884	enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5)	865:981	Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5 × 10(5) were achieved in 15 min using 10mM sulfobutyl ether-β-CD and 20mM methyl-β-CD.
24401457	1	74	theme	priority	247:254	arg1	pollutants					256:265	priority pollutants	247:265	priority pollutants	247:265	For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm).
26572352	12	0	theme	property	1894:1901	arg1	modulation					1903:1912	This material property modulation	1880:1912	This material property modulation	1880:1912	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	0	1	from	viscoelasticity	36:50	arg1	hydrogels					128:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	11	2	attach	presence	1728:1735	arg1	concentration					1773:1785	such minute concentration	1761:1785	such minute concentration	1761:1785	Thus, the presence of nanoclay laponite in such minute concentration is shown to cause considerable change in the thermo-physical property of the composite gels.
26572352	11	2	attach	presence	1728:1735	arg2	laponite					1749:1756	nanoclay laponite	1740:1756	nanoclay laponite	1740:1756	Thus, the presence of nanoclay laponite in such minute concentration is shown to cause considerable change in the thermo-physical property of the composite gels.
26572352	3	3	theme	hydrodynamic	530:541	arg1	radius					543:548	hydrodynamic radius	530:548	hydrodynamic radius	530:548	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	3	4	theme	light	580:584	arg1	data					597:600	light scattering data	580:600	light scattering data	580:600	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	4	5	theme	nanoclay	685:692	arg1	laponite					694:701	nanoclay laponite	685:701	nanoclay laponite	685:701	When nanoclay laponite was added to the pectin chains solution, two-phase separation was noticed instantaneously.
26572352	7	6	theme	loss	1114:1117	arg1	moduli					1119:1124	The storage and loss moduli	1098:1124	moduli	1119:1124	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	2	7	dep	kinetics	275:282	arg1	the					262:264	the	262:264	the	262:264	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	7	8	theme	storage	1102:1108	arg1	moduli					1119:1124	The storage and loss moduli	1098:1124	moduli	1119:1124	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	2	9	theme	polysaccharide	330:343	arg1	dispersion					352:361	anionic polysaccharide pectin dispersion	322:361	anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite	322:403	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	9	10	from	ergodic	1531:1537	arg1	transition					1515:1524	The transition	1511:1524	The transition from ergodic to non-ergodic state	1511:1558	The transition from ergodic to non-ergodic state occurred during sol-gel transition owing to the presence of the nanoclay laponite.
26572352	12	11	theme	varying	1989:1995	arg1	modulus					1968:1974	storage modulus	1960:1974	storage modulus continuously varying	1960:1995	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	3	12	theme	coils	634:638	arg1	presence					615:622	the presence	611:622	the presence of random coils of pectin chains inside the gel matrix	611:677	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	6	13	theme	cetyltrimethylammonium	968:989	arg1	[CTAB					1000:1004	[CTAB	1000:1004	[CTAB	1000:1004	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	6	13	theme	cetyltrimethylammonium	968:989	arg1	bromide					991:997	The exfoliating agent cetyltrimethylammonium bromide	946:997	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10)	946:1014	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	0	14	from	ergodicity	71:80	arg1	hydrogels					128:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	12	15	theme	soft	1943:1946	arg1	gels					1948:1951	soft gels	1943:1951	soft gels having storage modulus continuously varying in the wide range of 10-70 Pa	1943:2025	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	6	16	theme	exfoliating	950:960	arg1	[CTAB					1000:1004	[CTAB	1000:1004	[CTAB	1000:1004	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	6	16	theme	exfoliating	950:960	arg1	bromide					991:997	The exfoliating agent cetyltrimethylammonium bromide	946:997	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10)	946:1014	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	8	17	theme	nanoclay	1396:1403	arg1	laponite					1405:1412	nanoclay laponite	1396:1412	nanoclay laponite	1396:1412	However, as the concentration of nanoclay laponite was maintained between 0.01% and 0.03% (w/v), the gel rigidity (G') recovered by 30% (35-45 Pa).
26572352	4	18	theme	two-phase	744:752	arg1	separation					754:763	two-phase separation	744:763	two-phase separation	744:763	When nanoclay laponite was added to the pectin chains solution, two-phase separation was noticed instantaneously.
26572352	2	19	theme	gelation	266:273	arg1	kinetics					275:282	gelation kinetics	266:282	gelation kinetics	266:282	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	1	20	theme	viscoelastic	165:176	arg1	properties					178:187	the viscoelastic properties	161:187	the viscoelastic properties of pectin gels	161:202	In order to customize the viscoelastic properties of pectin gels, it is necessary to work on a composite platform.
26572352	0	21	from	hydrogels	128:136	arg1	Characterization					0:15	Characterization	0:15	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.	0:137	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	7	22	theme	gel	1293:1295	arg1	strength					1297:1304	gel strength	1293:1304	gel strength	1293:1304	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	7	23	theme	content	1214:1220	arg1	function					1184:1191	function	1184:1191	function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel	1184:1360	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	3	24	theme	pectin	643:648	arg1	chains					650:655	pectin chains	643:655	pectin chains inside the gel matrix	643:677	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	0	25	from	microstructure	20:33	arg1	hydrogels					128:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	4	26	theme	pectin	720:725	arg1	solution					734:741	the pectin chains solution	716:741	the pectin chains solution	716:741	When nanoclay laponite was added to the pectin chains solution, two-phase separation was noticed instantaneously.
26572352	0	27	theme	pectin-laponite-CTAB-calcium	85:112	arg1	hydrogels					128:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	1	28	theme	gels	199:202	arg1	properties					178:187	the viscoelastic properties	161:187	the viscoelastic properties of pectin gels	161:202	In order to customize the viscoelastic properties of pectin gels, it is necessary to work on a composite platform.
26572352	9	29	theme	laponite	1633:1640	arg1	presence					1608:1615	the presence	1604:1615	the presence of the nanoclay laponite	1604:1640	The transition from ergodic to non-ergodic state occurred during sol-gel transition owing to the presence of the nanoclay laponite.
26572352	0	30	from	Characterization	0:15	arg1	hydrogels					128:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	2	31	theme	viscoelastic	289:300	arg1	characterization					302:317	viscoelastic characterization	289:317	viscoelastic characterization	289:317	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	7	32	dep	%	1250:1250	arg1	0.03					1246:1249	0.03	1246:1249	0.03	1246:1249	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	7	32	dep	%	1250:1250	arg1	w/v					1253:1255	w/v	1253:1255	w/v	1253:1255	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	0	33	from	heterogeneity	53:65	arg1	hydrogels					128:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	2	34	theme	rheology	453:460	arg1	measurements					462:473	dynamic light scattering and rheology measurements	424:473	dynamic light scattering and rheology measurements	424:473	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	2	35	theme	light	432:436	arg1	scattering					438:447	dynamic light scattering	424:447	dynamic light scattering	424:447	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	6	36	theme	gels	1092:1095	arg1	stability					1054:1062	stability	1054:1062	stability	1054:1062	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	6	36	theme	gels	1092:1095	arg1	homogeneity					1038:1048	homogeneity	1038:1048	homogeneity	1038:1048	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	6	37	dep	homogeneity	1038:1048	arg1	the					1034:1036	the	1034:1036	the	1034:1036	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	12	38	theme	unaltered	2073:2081	arg1	temperature					2054:2064	the gelation temperature	2041:2064	the gelation temperature mostly unaltered	2041:2081	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	11	39	theme	nanoclay	1740:1747	arg1	laponite					1749:1756	nanoclay laponite	1740:1756	nanoclay laponite	1740:1756	Thus, the presence of nanoclay laponite in such minute concentration is shown to cause considerable change in the thermo-physical property of the composite gels.
26572352	7	40	theme	gel	1155:1157	arg1	moduli					1119:1124	The storage and loss moduli	1098:1124	moduli	1119:1124	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	11	41	theme	gels	1874:1877	arg1	property					1848:1855	the thermo-physical property	1828:1855	the thermo-physical property of the composite gels	1828:1877	Thus, the presence of nanoclay laponite in such minute concentration is shown to cause considerable change in the thermo-physical property of the composite gels.
26572352	7	42	dep	concentration	1226:1238	arg1	%					1250:1250	%	1250:1250	%	1250:1250	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	6	43	theme	sols	1083:1086	arg1	stability					1054:1062	stability	1054:1062	stability	1054:1062	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	6	43	theme	sols	1083:1086	arg1	homogeneity					1038:1048	homogeneity	1038:1048	homogeneity	1038:1048	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	0	44	theme	microstructure	20:33	arg1	Characterization					0:15	Characterization	0:15	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.	0:137	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	0	45	theme	ergodicity	71:80	arg1	Characterization					0:15	Characterization	0:15	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.	0:137	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	11	46	theme	minute	1766:1771	arg1	concentration					1773:1785	such minute concentration	1761:1785	such minute concentration	1761:1785	Thus, the presence of nanoclay laponite in such minute concentration is shown to cause considerable change in the thermo-physical property of the composite gels.
26572352	0	47	theme	viscoelasticity	36:50	arg1	Characterization					0:15	Characterization	0:15	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.	0:137	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	5	48	theme	cetyltrimethylammonium	820:841	arg1	bromide					843:849	the surfactant cetyltrimethylammonium bromide [CTAB]	805:856	the surfactant cetyltrimethylammonium bromide [CTAB]	805:856	Therefore, the surfactant cetyltrimethylammonium bromide [CTAB] was added to exfoliate the clay platelets in the dispersion, and also in its gel phase.
26572352	8	49	theme	laponite	1405:1412	arg1	concentration					1379:1391	the concentration	1375:1391	the concentration of nanoclay laponite	1375:1412	However, as the concentration of nanoclay laponite was maintained between 0.01% and 0.03% (w/v), the gel rigidity (G') recovered by 30% (35-45 Pa).
26572352	3	50	theme	gel	668:670	arg1	matrix					672:677	the gel matrix	664:677	the gel matrix	664:677	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	7	51	theme	G	1134:1134	arg1	"					1135:1135	G"	1134:1135	G"	1134:1135	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	3	52	dep	Rg/Rh	486:490	arg1	Rg					493:494	Rg	493:494	Rg	493:494	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	3	52	dep	Rg/Rh	486:490	arg1	radius					507:512	Rh are radius	500:512	Rh are radius of gyration and hydrodynamic radius	500:548	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	1	53	theme	composite	234:242	arg1	platform					244:251	a composite platform	232:251	a composite platform	232:251	In order to customize the viscoelastic properties of pectin gels, it is necessary to work on a composite platform.
26572352	3	54	theme	radius	543:548	arg1	Rg					493:494	Rg	493:494	Rg	493:494	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	3	54	theme	radius	543:548	arg1	radius					507:512	Rh are radius	500:512	Rh are radius of gyration and hydrodynamic radius	500:548	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	2	55	theme	nanoclay	387:394	arg1	laponite					396:403	nanoclay laponite	387:403	nanoclay laponite	387:403	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	12	56	theme	material	1885:1892	arg1	modulation					1903:1912	This material property modulation	1880:1912	This material property modulation	1880:1912	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	10	57	theme	breaking	1703:1710	arg1	time					1712:1715	the ergodicity breaking time	1688:1715	the ergodicity breaking time	1688:1715	The gelation time was not too different from the ergodicity breaking time.
26572352	11	58	theme	composite	1864:1872	arg1	gels					1874:1877	the composite gels	1860:1877	the composite gels	1860:1877	Thus, the presence of nanoclay laponite in such minute concentration is shown to cause considerable change in the thermo-physical property of the composite gels.
26572352	10	59	theme	gelation	1647:1654	arg1	time					1656:1659	The gelation time	1643:1659	The gelation time	1643:1659	The gelation time was not too different from the ergodicity breaking time.
26572352	10	59	theme	gelation	1647:1654	arg1	different					1673:1681	different	1673:1681	different	1673:1681	The gelation time was not too different from the ergodicity breaking time.
26572352	0	60	theme	heterogeneity	53:65	arg1	Characterization					0:15	Characterization	0:15	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.	0:137	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	7	61	dep	moduli	1119:1124	arg1	"					1135:1135	G"	1134:1135	G"	1134:1135	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	7	61	dep	moduli	1119:1124	arg1	G					1127:1127	G'	1127:1128	G'	1127:1128	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	7	62	theme	nanoclay	1196:1203	arg1	content					1214:1220	nanoclay laponite content	1196:1220	nanoclay laponite content	1196:1220	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	2	63	theme	pectin	345:350	arg1	dispersion					352:361	anionic polysaccharide pectin dispersion	322:361	anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite	322:403	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	7	64	dep	50	1326:1327	arg1	to					1323:1324	to	1323:1324	to	1323:1324	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	9	65	theme	non-ergodic	1542:1552	arg1	state					1554:1558	non-ergodic state	1542:1558	non-ergodic state	1542:1558	The transition from ergodic to non-ergodic state occurred during sol-gel transition owing to the presence of the nanoclay laponite.
26572352	7	66	theme	pectin-calcium	1343:1356	arg1	gel					1358:1360	pectin-calcium gel	1343:1360	pectin-calcium gel	1343:1360	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	12	67	theme	wide	2004:2007	arg1	range					2009:2013	the wide range	2000:2013	the wide range of 10-70 Pa	2000:2025	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	2	68	theme	anionic	322:328	arg1	dispersion					352:361	anionic polysaccharide pectin dispersion	322:361	anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite	322:403	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	12	69	theme	storage	1960:1966	arg1	modulus					1968:1974	storage modulus	1960:1974	storage modulus continuously varying	1960:1995	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	3	70	theme	random	627:632	arg1	coils					634:638	random coils	627:638	random coils of pectin chains inside the gel matrix	627:677	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	5	71	theme	clay	885:888	arg1	platelets					890:898	the clay platelets	881:898	the clay platelets	881:898	Therefore, the surfactant cetyltrimethylammonium bromide [CTAB] was added to exfoliate the clay platelets in the dispersion, and also in its gel phase.
26572352	9	72	theme	sol-gel	1576:1582	arg1	transition					1584:1593	sol-gel transition	1576:1593	sol-gel transition owing to the presence of the nanoclay laponite	1576:1640	The transition from ergodic to non-ergodic state occurred during sol-gel transition owing to the presence of the nanoclay laponite.
26572352	11	73	theme	thermo-physical	1832:1846	arg1	property					1848:1855	the thermo-physical property	1828:1855	the thermo-physical property of the composite gels	1828:1877	Thus, the presence of nanoclay laponite in such minute concentration is shown to cause considerable change in the thermo-physical property of the composite gels.
26572352	5	74	theme	gel	935:937	arg1	phase					939:943	its gel phase	931:943	its gel phase	931:943	Therefore, the surfactant cetyltrimethylammonium bromide [CTAB] was added to exfoliate the clay platelets in the dispersion, and also in its gel phase.
26572352	6	75	theme	agent	962:966	arg1	[CTAB					1000:1004	[CTAB	1000:1004	[CTAB	1000:1004	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	6	75	theme	agent	962:966	arg1	bromide					991:997	The exfoliating agent cetyltrimethylammonium bromide	946:997	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10)	946:1014	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	12	76	contain	having	1953:1958	arg1	gels					1948:1951	soft gels	1943:1951	soft gels having storage modulus continuously varying in the wide range of 10-70 Pa	1943:2025	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	12	76	contain	having	1953:1958	arg2	modulus					1968:1974	storage modulus	1960:1974	storage modulus continuously varying	1960:1995	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	6	77	dep	[CTAB	1000:1004	arg1	cmc/10					1008:1013	≈ cmc/10	1006:1013	≈ cmc/10	1006:1013	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	4	78	theme	chains	727:732	arg1	solution					734:741	the pectin chains solution	716:741	the pectin chains solution	716:741	When nanoclay laponite was added to the pectin chains solution, two-phase separation was noticed instantaneously.
26572352	10	79	theme	ergodicity	1692:1701	arg1	time					1712:1715	the ergodicity breaking time	1688:1715	the ergodicity breaking time	1688:1715	The gelation time was not too different from the ergodicity breaking time.
26572352	3	80	theme	scattering	586:595	arg1	data					597:600	light scattering data	580:600	light scattering data	580:600	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	12	81	theme	gels	1948:1951	arg1	designing					1930:1938	designing	1930:1938	designing	1930:1938	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	7	82	theme	laponite	1205:1212	arg1	content					1214:1220	nanoclay laponite content	1196:1220	nanoclay laponite content	1196:1220	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	0	83	theme	nanocomposite	114:126	arg1	hydrogels					128:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	pectin-laponite-CTAB-calcium nanocomposite hydrogels	85:136	Characterization of microstructure, viscoelasticity, heterogeneity and ergodicity in pectin-laponite-CTAB-calcium nanocomposite hydrogels.
26572352	1	84	theme	pectin	192:197	arg1	gels					199:202	pectin gels	192:202	pectin gels	192:202	In order to customize the viscoelastic properties of pectin gels, it is necessary to work on a composite platform.
26572352	9	85	theme	nanoclay	1624:1631	arg1	laponite					1633:1640	the nanoclay laponite	1620:1640	the nanoclay laponite	1620:1640	The transition from ergodic to non-ergodic state occurred during sol-gel transition owing to the presence of the nanoclay laponite.
26572352	7	86	dep	0.03	1246:1249	arg1	to					1243:1244	to	1243:1244	to	1243:1244	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	3	87	dep	radius	507:512	arg1	are					503:505	are	503:505	are	503:505	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	11	88	from	presence	1728:1735	arg1	concentration					1773:1785	such minute concentration	1761:1785	such minute concentration	1761:1785	Thus, the presence of nanoclay laponite in such minute concentration is shown to cause considerable change in the thermo-physical property of the composite gels.
26572352	7	89	theme	gels	1287:1290	arg1	softening					1270:1278	the softening	1266:1278	the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel	1266:1360	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	2	90	theme	dynamic	424:430	arg1	scattering					438:447	dynamic light scattering	424:447	dynamic light scattering	424:447	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	7	91	theme	composite	1145:1153	arg1	gel					1155:1157	the composite gel	1141:1157	the composite gel	1141:1157	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	3	92	theme	chains	650:655	arg1	coils					634:638	random coils	627:638	random coils of pectin chains inside the gel matrix	627:677	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	11	93	theme	laponite	1749:1756	arg1	presence					1728:1735	the presence	1724:1735	the presence of nanoclay laponite in such minute concentration	1724:1785	Thus, the presence of nanoclay laponite in such minute concentration is shown to cause considerable change in the thermo-physical property of the composite gels.
26572352	11	94	theme	considerable	1805:1816	arg1	change					1818:1823	considerable change	1805:1823	considerable change	1805:1823	Thus, the presence of nanoclay laponite in such minute concentration is shown to cause considerable change in the thermo-physical property of the composite gels.
26572352	6	95	theme	pectin-clay	1071:1081	arg1	sols					1083:1086	the pectin-clay sols	1067:1086	the pectin-clay sols	1067:1086	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	8	96	theme	gel	1464:1466	arg1	G					1478:1478	G'	1478:1479	G'	1478:1479	However, as the concentration of nanoclay laponite was maintained between 0.01% and 0.03% (w/v), the gel rigidity (G') recovered by 30% (35-45 Pa).
26572352	8	96	theme	gel	1464:1466	arg1	rigidity					1468:1475	the gel rigidity	1460:1475	the gel rigidity (G')	1460:1480	However, as the concentration of nanoclay laponite was maintained between 0.01% and 0.03% (w/v), the gel rigidity (G') recovered by 30% (35-45 Pa).
26572352	3	97	theme	Rh	500:501	arg1	radius					507:512	Rh are radius	500:512	Rh are radius of gyration and hydrodynamic radius	500:548	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	5	98	theme	surfactant	809:818	arg1	bromide					843:849	the surfactant cetyltrimethylammonium bromide [CTAB]	805:856	the surfactant cetyltrimethylammonium bromide [CTAB]	805:856	Therefore, the surfactant cetyltrimethylammonium bromide [CTAB] was added to exfoliate the clay platelets in the dispersion, and also in its gel phase.
26572352	3	99	theme	ratio	480:484	arg1	Rg/Rh					486:490	The ratio Rg/Rh	476:490	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data	476:600	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	2	100	theme	scattering	438:447	arg1	measurements					462:473	dynamic light scattering and rheology measurements	424:473	dynamic light scattering and rheology measurements	424:473	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	7	101	dep	gels	1287:1290	arg1	reduced					1306:1312	reduced	1306:1312	reduced by close to 50%	1306:1328	The storage and loss moduli (G' and G") of the composite gel changed significantly as function of nanoclay laponite content for concentration up to 0.03% (w/v) causing the softening of the gels (gel strength reduced by close to 50%) compared to pectin-calcium gel.
26572352	12	102	theme	gelation	2045:2052	arg1	temperature					2054:2064	the gelation temperature	2041:2064	the gelation temperature mostly unaltered	2041:2081	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	3	103	theme	gyration	517:524	arg1	Rg					493:494	Rg	493:494	Rg	493:494	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	3	103	theme	gyration	517:524	arg1	radius					507:512	Rh are radius	500:512	Rh are radius of gyration and hydrodynamic radius	500:548	The ratio Rg/Rh (Rg and Rh are radius of gyration and hydrodynamic radius respectively) determined from light scattering data revealed the presence of random coils of pectin chains inside the gel matrix.
26572352	6	104	gly	homogeneity	1038:1048	arg1	gels					1092:1095	gels	1092:1095	gels	1092:1095	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	6	104	gly	homogeneity	1038:1048	arg1	sols					1083:1086	the pectin-clay sols	1067:1086	the pectin-clay sols	1067:1086	The exfoliating agent cetyltrimethylammonium bromide ([CTAB]≈ cmc/10) helped to enhance the homogeneity and stability of the pectin-clay sols and gels.
26572352	2	105	theme	dispersion	352:361	arg1	characterization					302:317	viscoelastic characterization	289:317	viscoelastic characterization	289:317	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	2	105	theme	dispersion	352:361	arg1	kinetics					275:282	gelation kinetics	266:282	gelation kinetics	266:282	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
26572352	10	106	from	time	1712:1715	arg1	time					1656:1659	The gelation time	1643:1659	The gelation time	1643:1659	The gelation time was not too different from the ergodicity breaking time.
26572352	10	106	from	time	1712:1715	arg1	different					1673:1681	different	1673:1681	different	1673:1681	The gelation time was not too different from the ergodicity breaking time.
26572352	11	107	theme	such	1761:1764	arg1	concentration					1773:1785	such minute concentration	1761:1785	such minute concentration	1761:1785	Thus, the presence of nanoclay laponite in such minute concentration is shown to cause considerable change in the thermo-physical property of the composite gels.
26572352	12	108	theme	Pa	2024:2025	arg1	range					2009:2013	the wide range	2000:2013	the wide range of 10-70 Pa	2000:2025	This material property modulation will facilitate designing of soft gels having storage modulus continuously varying in the wide range of 10-70 Pa while keeping the gelation temperature mostly unaltered.
26572352	2	109	theme	laponite	396:403	arg1	presence					375:382	presence	375:382	presence of nanoclay laponite	375:403	Herein, the gelation kinetics, and viscoelastic characterization of anionic polysaccharide pectin dispersion prepared in presence of nanoclay laponite are reported using dynamic light scattering and rheology measurements.
24594051	7	0	theme	standardized	1120:1131	arg1	guidelines					1133:1142	the standardized guidelines	1116:1142	the standardized guidelines of the International Knee Documentation Committee	1116:1192	Patients were evaluated using the standardized guidelines of the International Knee Documentation Committee.
24594051	4	1	theme	differentiated	712:725	arg1	chondrocytes					727:738	differentiated chondrocytes	712:738	differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin	712:789	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	9	2	theme	homogeneous	1424:1434	arg1	patients					1436:1443	homogeneous patients	1424:1443	homogeneous patients	1424:1443	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	8	3	theme	histological	1324:1335	arg1	makeup					1337:1342	the histological makeup	1320:1342	the histological makeup of the newly formed cartilage	1320:1372	During the comparison of bioptic samples obtained from both patient cohorts, we did not observe any important differences in the histological makeup of the newly formed cartilage.
24594051	9	4	dep	matrix	1597:1602	arg1	the					1583:1585	the	1583:1585	the	1583:1585	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	9	5	theme	cellular	1608:1615	arg1	composition					1617:1627	cellular composition	1608:1627	cellular composition	1608:1627	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	5	6	theme	biomaterial	894:904	arg1	fibres					906:911	biomaterial fibres	894:911	biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin	894:1038	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	1	7	theme	histological	163:174	arg1	composition					209:219	the histological and immunohistochemical cellular composition	159:219	the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation	159:309	Our study compares the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation.
24594051	5	8	theme	hyaluronic	930:939	arg1	acid					941:944	hyaluronic acid	930:944	hyaluronic acid	930:944	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	7	9	theme	Documentation	1170:1182	arg1	Committee					1184:1192	the International Knee Documentation Committee	1147:1192	the International Knee Documentation Committee	1147:1192	Patients were evaluated using the standardized guidelines of the International Knee Documentation Committee.
24594051	9	10	theme	clinical	1543:1550	arg1	results					1552:1558	favourable and stable clinical results	1521:1558	favourable and stable clinical results	1521:1558	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	8	11	theme	formed	1357:1362	arg1	cartilage					1364:1372	the newly formed cartilage	1347:1372	the newly formed cartilage	1347:1372	During the comparison of bioptic samples obtained from both patient cohorts, we did not observe any important differences in the histological makeup of the newly formed cartilage.
24594051	5	12	theme	cells	950:954	arg1	esters					920:925	the esters	916:925	the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin	916:1038	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	1	13	theme	transplantation	295:309	arg1	biomaterials					257:268	two different chondrocyte-seeded biomaterials	224:268	two different chondrocyte-seeded biomaterials	224:268	Our study compares the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation.
24594051	1	13	theme	transplantation	295:309	arg1	results					278:284	the results	274:284	the results of their transplantation	274:309	Our study compares the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation.
24594051	0	14	theme	results	96:102	arg1	composition					27:37	the cellular composition	14:37	the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans	14:137	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	3	15	theme	arthroscopic	541:552	arg1	implantation					554:565	arthroscopic implantation	541:565	arthroscopic implantation of the tissue Hyalograft C and/or Brno culture	541:612	These patients were enrolled in our study and treated with arthroscopic implantation of the tissue Hyalograft C and/or Brno culture.
24594051	9	16	theme	histological	1379:1390	arg1	analysis					1392:1399	The histological analysis	1375:1399	The histological analysis of these two groups of homogeneous patients	1375:1443	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	0	17	theme	transplantation	113:127	arg1	biomaterials					75:86	two different chondrocyte-seeded biomaterials	42:86	two different chondrocyte-seeded biomaterials	42:86	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	0	17	theme	transplantation	113:127	arg1	results					96:102	the results	92:102	the results	92:102	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	9	18	theme	groups	1414:1419	arg1	analysis					1392:1399	The histological analysis	1375:1399	The histological analysis of these two groups of homogeneous patients	1375:1443	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	7	19	theme	Committee	1184:1192	arg1	guidelines					1133:1142	the standardized guidelines	1116:1142	the standardized guidelines of the International Knee Documentation Committee	1116:1192	Patients were evaluated using the standardized guidelines of the International Knee Documentation Committee.
24594051	9	20	theme	proper	1491:1496	arg1	indications					1498:1508	proper indications	1491:1508	proper indications	1491:1508	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	2	21	theme	study	316:320	arg1	cohort					322:327	Our study cohort	312:327	Our study cohort	312:327	Our study cohort included 21 patients, comprising 19 men and two women with a mean age of 32 years, who were affected by single chondral lesions of the femoral condyles.
24594051	5	22	theme	fibrillar	864:872	arg1	network					874:880	a fibrillar network	862:880	a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin	862:1038	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	2	23	theme	years	405:409	arg1	age					395:397	a mean age	388:397	a mean age of 32 years	388:409	Our study cohort included 21 patients, comprising 19 men and two women with a mean age of 32 years, who were affected by single chondral lesions of the femoral condyles.
24594051	7	24	theme	International	1151:1163	arg1	Committee					1184:1192	the International Knee Documentation Committee	1147:1192	the International Knee Documentation Committee	1147:1192	Patients were evaluated using the standardized guidelines of the International Knee Documentation Committee.
24594051	5	25	theme	C	831:831	arg1	case					812:815	the case	808:815	the case of Hyalograft C	808:831	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	1	26	theme	immunohistochemical	180:198	arg1	composition					209:219	the histological and immunohistochemical cellular composition	159:219	the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation	159:309	Our study compares the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation.
24594051	5	27	theme	Hyalograft	820:829	arg1	C					831:831	Hyalograft C	820:831	Hyalograft C	820:831	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	4	28	theme	α-smooth	769:776	arg1	actin					785:789	α-smooth muscle actin	769:789	α-smooth muscle actin	769:789	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	1	29	theme	cellular	200:207	arg1	composition					209:219	the histological and immunohistochemical cellular composition	159:219	the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation	159:309	Our study compares the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation.
24594051	2	30	theme	chondral	440:447	arg1	lesions					449:455	single chondral lesions	433:455	single chondral lesions of the femoral condyles	433:479	Our study cohort included 21 patients, comprising 19 men and two women with a mean age of 32 years, who were affected by single chondral lesions of the femoral condyles.
24594051	7	31	theme	Knee	1165:1168	arg1	Committee					1184:1192	the International Knee Documentation Committee	1147:1192	the International Knee Documentation Committee	1147:1192	Patients were evaluated using the standardized guidelines of the International Knee Documentation Committee.
24594051	4	32	contain	contains	671:678	arg2	cells					686:690	round cells	680:690	round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin	680:789	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	4	32	contain	contains	671:678	arg1	culture					620:626	Brno culture bioengineered	615:640	Brno culture bioengineered with a fibrin-based scaffold	615:669	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	3	33	theme	tissue	574:579	arg1	C					592:592	the tissue Hyalograft C and/or Brno culture	570:612	C	592:592	These patients were enrolled in our study and treated with arthroscopic implantation of the tissue Hyalograft C and/or Brno culture.
24594051	4	34	theme	round	680:684	arg1	cells					686:690	round cells	680:690	round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin	680:789	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	0	35	theme	cellular	18:25	arg1	composition					27:37	the cellular composition	14:37	the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans	14:137	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	9	36	theme	transplants	1640:1650	arg1	composition					1617:1627	cellular composition	1608:1627	cellular composition	1608:1627	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	9	36	theme	transplants	1640:1650	arg1	matrix					1597:1602	different matrix	1587:1602	different matrix	1587:1602	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	8	37	theme	bioptic	1220:1226	arg1	samples					1228:1234	bioptic samples	1220:1234	bioptic samples obtained from both patient cohorts	1220:1269	During the comparison of bioptic samples obtained from both patient cohorts, we did not observe any important differences in the histological makeup of the newly formed cartilage.
24594051	9	38	theme	different	1587:1595	arg1	matrix					1597:1602	different matrix	1587:1602	different matrix	1587:1602	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	5	39	theme	muscle	1027:1032	arg1	actin					1034:1038	only α-smooth muscle actin	1013:1038	only α-smooth muscle actin	1013:1038	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	5	40	theme	acid	941:944	arg1	esters					920:925	the esters	916:925	the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin	916:1038	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	9	41	theme	favourable	1521:1530	arg1	results					1552:1558	favourable and stable clinical results	1521:1558	favourable and stable clinical results	1521:1558	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	2	42	with	men	365:367	arg1	age					395:397	a mean age	388:397	a mean age of 32 years	388:409	Our study cohort included 21 patients, comprising 19 men and two women with a mean age of 32 years, who were affected by single chondral lesions of the femoral condyles.
24594051	4	43	theme	muscle	778:783	arg1	actin					785:789	α-smooth muscle actin	769:789	α-smooth muscle actin	769:789	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	4	44	theme	bioengineered	628:640	arg1	culture					620:626	Brno culture bioengineered	615:640	Brno culture bioengineered with a fibrin-based scaffold	615:669	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	2	45	theme	condyles	472:479	arg1	lesions					449:455	single chondral lesions	433:455	single chondral lesions of the femoral condyles	433:479	Our study cohort included 21 patients, comprising 19 men and two women with a mean age of 32 years, who were affected by single chondral lesions of the femoral condyles.
24594051	8	46	theme	important	1295:1303	arg1	differences					1305:1315	any important differences	1291:1315	any important differences	1291:1315	During the comparison of bioptic samples obtained from both patient cohorts, we did not observe any important differences in the histological makeup of the newly formed cartilage.
24594051	4	47	theme	fibrin-based	649:660	arg1	scaffold					662:669	a fibrin-based scaffold	647:669	a fibrin-based scaffold	647:669	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	4	48	with	culture	620:626	arg1	scaffold					662:669	a fibrin-based scaffold	647:669	a fibrin-based scaffold	647:669	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	6	49	theme	defects	1065:1071	arg1	size					1053:1056	The average size	1041:1056	The average size of the defects	1041:1071	The average size of the defects was 2.5 cm2.
24594051	6	49	theme	defects	1065:1071	arg1	cm2					1081:1083	2.5 cm2	1077:1083	2.5 cm2	1077:1083	The average size of the defects was 2.5 cm2.
24594051	1	50	theme	different	228:236	arg1	biomaterials					257:268	two different chondrocyte-seeded biomaterials	224:268	two different chondrocyte-seeded biomaterials	224:268	Our study compares the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation.
24594051	0	51	theme	composition	27:37	arg1	Comparison					0:9	Comparison	0:9	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.	0:138	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	2	52	theme	femoral	464:470	arg1	condyles					472:479	the femoral condyles	460:479	the femoral condyles	460:479	Our study cohort included 21 patients, comprising 19 men and two women with a mean age of 32 years, who were affected by single chondral lesions of the femoral condyles.
24594051	0	53	from	biomaterials	75:86	arg1	humans					132:137	humans	132:137	humans	132:137	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	2	54	theme	single	433:438	arg1	lesions					449:455	single chondral lesions	433:455	single chondral lesions of the femoral condyles	433:479	Our study cohort included 21 patients, comprising 19 men and two women with a mean age of 32 years, who were affected by single chondral lesions of the femoral condyles.
24594051	1	55	theme	chondrocyte-seeded	238:255	arg1	biomaterials					257:268	two different chondrocyte-seeded biomaterials	224:268	two different chondrocyte-seeded biomaterials	224:268	Our study compares the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation.
24594051	0	56	theme	different	46:54	arg1	biomaterials					75:86	two different chondrocyte-seeded biomaterials	42:86	two different chondrocyte-seeded biomaterials	42:86	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	9	57	theme	bioengineered	1461:1473	arg1	approach					1475:1482	this bioengineered approach	1456:1482	this bioengineered approach	1456:1482	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	6	58	theme	average	1045:1051	arg1	size					1053:1056	The average size	1041:1056	The average size of the defects	1041:1071	The average size of the defects was 2.5 cm2.
24594051	6	58	theme	average	1045:1051	arg1	cm2					1081:1083	2.5 cm2	1077:1083	2.5 cm2	1077:1083	The average size of the defects was 2.5 cm2.
24594051	3	59	theme	Hyalograft	581:590	arg1	C					592:592	the tissue Hyalograft C and/or Brno culture	570:612	C	592:592	These patients were enrolled in our study and treated with arthroscopic implantation of the tissue Hyalograft C and/or Brno culture.
24594051	0	60	from	transplantation	113:127	arg1	humans					132:137	humans	132:137	humans	132:137	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	3	61	theme	C	592:592	arg1	implantation					554:565	arthroscopic implantation	541:565	arthroscopic implantation of the tissue Hyalograft C and/or Brno culture	541:612	These patients were enrolled in our study and treated with arthroscopic implantation of the tissue Hyalograft C and/or Brno culture.
24594051	4	62	theme	chondrocytes	727:738	arg1	features					700:707	features	700:707	features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin	700:789	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	5	63	theme	α-smooth	1018:1025	arg1	actin					1034:1038	only α-smooth muscle actin	1013:1038	only α-smooth muscle actin	1013:1038	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	2	64	with	women	377:381	arg1	age					395:397	a mean age	388:397	a mean age of 32 years	388:409	Our study cohort included 21 patients, comprising 19 men and two women with a mean age of 32 years, who were affected by single chondral lesions of the femoral condyles.
24594051	3	65	theme	Brno	601:604	arg1	culture					606:612	the tissue Hyalograft C and/or Brno culture	570:612	culture	606:612	These patients were enrolled in our study and treated with arthroscopic implantation of the tissue Hyalograft C and/or Brno culture.
24594051	5	66	theme	esters	920:925	arg1	fibres					906:911	biomaterial fibres	894:911	biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin	894:1038	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	1	67	theme	biomaterials	257:268	arg1	composition					209:219	the histological and immunohistochemical cellular composition	159:219	the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation	159:309	Our study compares the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation.
24594051	8	68	theme	cartilage	1364:1372	arg1	makeup					1337:1342	the histological makeup	1320:1342	the histological makeup of the newly formed cartilage	1320:1372	During the comparison of bioptic samples obtained from both patient cohorts, we did not observe any important differences in the histological makeup of the newly formed cartilage.
24594051	9	69	theme	patients	1436:1443	arg1	groups					1414:1419	these two groups	1404:1419	these two groups of homogeneous patients	1404:1443	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	9	69	theme	patients	1436:1443	arg1	patients					1436:1443	homogeneous patients	1424:1443	homogeneous patients	1424:1443	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	4	70	theme	S-100	751:755	arg1	protein					757:763	S-100 protein	751:763	S-100 protein	751:763	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	8	71	theme	samples	1228:1234	arg1	comparison					1206:1215	the comparison	1202:1215	the comparison of bioptic samples obtained from both patient cohorts	1202:1269	During the comparison of bioptic samples obtained from both patient cohorts, we did not observe any important differences in the histological makeup of the newly formed cartilage.
24594051	0	72	theme	biomaterials	75:86	arg1	composition					27:37	the cellular composition	14:37	the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans	14:137	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	0	73	from	results	96:102	arg1	humans					132:137	humans	132:137	humans	132:137	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	3	74	theme	culture	606:612	arg1	implantation					554:565	arthroscopic implantation	541:565	arthroscopic implantation of the tissue Hyalograft C and/or Brno culture	541:612	These patients were enrolled in our study and treated with arthroscopic implantation of the tissue Hyalograft C and/or Brno culture.
24594051	9	75	theme	stable	1536:1541	arg1	results					1552:1558	favourable and stable clinical results	1521:1558	favourable and stable clinical results	1521:1558	The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
24594051	2	76	theme	mean	390:393	arg1	age					395:397	a mean age	388:397	a mean age of 32 years	388:409	Our study cohort included 21 patients, comprising 19 men and two women with a mean age of 32 years, who were affected by single chondral lesions of the femoral condyles.
24594051	8	77	theme	patient	1255:1261	arg1	cohorts					1263:1269	both patient cohorts	1250:1269	both patient cohorts	1250:1269	During the comparison of bioptic samples obtained from both patient cohorts, we did not observe any important differences in the histological makeup of the newly formed cartilage.
24594051	0	78	from	humans	132:137	arg1	biomaterials					75:86	two different chondrocyte-seeded biomaterials	42:86	two different chondrocyte-seeded biomaterials	42:86	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	0	78	from	humans	132:137	arg1	results					96:102	the results	92:102	the results	92:102	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	0	79	theme	chondrocyte-seeded	56:73	arg1	biomaterials					75:86	two different chondrocyte-seeded biomaterials	42:86	two different chondrocyte-seeded biomaterials	42:86	Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
24594051	4	80	theme	Brno	615:618	arg1	culture					620:626	Brno culture bioengineered	615:640	Brno culture bioengineered with a fibrin-based scaffold	615:669	Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin.
24594051	5	81	dep	cells	950:954	arg1	expressing					1002:1011	expressing	1002:1011	expressing only α-smooth muscle actin	1002:1038	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	5	81	dep	cells	950:954	arg1	resembling					956:965	resembling	956:965	resembling fibroblasts and myofibroblasts	956:996	In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin.
24594051	1	82	theme	results	278:284	arg1	composition					209:219	the histological and immunohistochemical cellular composition	159:219	the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation	159:309	Our study compares the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation.
27987828	1	0	theme	sepharose	317:325	arg1	chromatography					337:350	sepharose 6B column chromatography	317:350	sepharose 6B column chromatography	317:350	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	4	1	theme	α-d-glucopyranosyl	831:848	arg1	residue					850:856	terminal α-d-glucopyranosyl residue	822:856	terminal α-d-glucopyranosyl residue	822:856	The backbone of MGPS was composed of two (1→3)-β-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue.
27987828	1	2	dep	tubing	287:292	arg1	membrane					304:311	cellulose membrane	294:311	cellulose membrane	294:311	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	4	3	theme	-α-d-glucopyranosyl	871:889	arg1	residue					891:897	one (1→4)-α-d-glucopyranosyl residue	862:897	one (1→4)-α-d-glucopyranosyl residue	862:897	The backbone of MGPS was composed of two (1→3)-β-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue.
27987828	0	4	from	characterization	11:26	arg1	giganteus					80:88	Meripilus giganteus	70:88	Meripilus giganteus	70:88	Structural characterization and antioxidant activity of a glucan from Meripilus giganteus.
27987828	4	5	theme	terminal	822:829	arg1	residue					850:856	terminal α-d-glucopyranosyl residue	822:856	terminal α-d-glucopyranosyl residue	822:856	The backbone of MGPS was composed of two (1→3)-β-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue.
27987828	1	6	theme	molecular	133:141	arg1	weight					143:148	a molecular weight	131:148	a molecular weight ∼1.48×105Da	131:160	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	1	7	theme	hot	224:226	arg1	extraction					234:243	hot water extraction	224:243	hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography	224:350	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	1	8	theme	column	330:335	arg1	chromatography					337:350	sepharose 6B column chromatography	317:350	sepharose 6B column chromatography	317:350	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	4	9	theme	-α-d-glucopyranosyl	708:726	arg1	residues					728:735	one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue	699:963	residues	728:735	The backbone of MGPS was composed of two (1→3)-β-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue.
27987828	1	10	theme	water	228:232	arg1	extraction					234:243	hot water extraction	224:243	hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography	224:350	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	4	11	theme	O-6	911:913	arg1	position					915:922	O-6 position	911:922	O-6 position	911:922	The backbone of MGPS was composed of two (1→3)-β-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue.
27987828	5	12	theme	superoxide	1081:1090	arg1	radical					1092:1098	superoxide radical	1081:1098	superoxide radical scavenging activity (IC50=70μg/mL)	1081:1133	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	4	13	theme	terminal	929:936	arg1	residue					957:963	terminal β-d-glucopyranosyl residue	929:963	terminal β-d-glucopyranosyl residue	929:963	The backbone of MGPS was composed of two (1→3)-β-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue.
27987828	5	14	theme	radical	1092:1098	arg1	IC50=70μg/mL					1121:1132	IC50=70μg/mL	1121:1132	IC50=70μg/mL	1121:1132	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	5	14	theme	radical	1092:1098	arg1	activity					1111:1118	superoxide radical scavenging activity	1081:1118	superoxide radical scavenging activity (IC50=70μg/mL)	1081:1133	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	4	15	theme	O-6	804:806	arg1	position					808:815	O-6 position	804:815	O-6 position	804:815	The backbone of MGPS was composed of two (1→3)-β-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue.
27987828	1	16	theme	weight	143:148	arg1	∼1.48×105Da					150:160	a molecular weight ∼1.48×105Da	131:160	a molecular weight ∼1.48×105Da	131:160	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	5	17	theme	scavenging	1100:1109	arg1	IC50=70μg/mL					1121:1132	IC50=70μg/mL	1121:1132	IC50=70μg/mL	1121:1132	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	5	17	theme	scavenging	1100:1109	arg1	activity					1111:1118	superoxide radical scavenging activity	1081:1118	superoxide radical scavenging activity (IC50=70μg/mL)	1081:1133	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	0	18	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activity of a glucan from Meripilus giganteus.
27987828	5	19	theme	ion	1148:1150	arg1	IC50=290μg/mL					1172:1184	IC50=290μg/mL	1172:1184	IC50=290μg/mL	1172:1184	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	5	19	theme	ion	1148:1150	arg1	activity					1162:1169	ferrous ion chelating activity	1140:1169	ferrous ion chelating activity (IC50=290μg/mL)	1140:1185	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	0	20	from	activity	44:51	arg1	giganteus					80:88	Meripilus giganteus	70:88	Meripilus giganteus	70:88	Structural characterization and antioxidant activity of a glucan from Meripilus giganteus.
27987828	1	21	with	glucan	111:116	arg1	∼1.48×105Da					150:160	a molecular weight ∼1.48×105Da	131:160	a molecular weight ∼1.48×105Da	131:160	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	1	22	theme	6B	327:328	arg1	chromatography					337:350	sepharose 6B column chromatography	317:350	sepharose 6B column chromatography	317:350	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	1	23	theme	new	93:95	arg1	MGPS					119:122	MGPS	119:122	MGPS	119:122	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	1	23	theme	new	93:95	arg1	glucan					111:116	A new water soluble glucan	91:116	A new water soluble glucan (MGPS)	91:123	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	0	24	theme	antioxidant	32:42	arg1	activity					44:51	antioxidant activity	32:51	antioxidant activity	32:51	Structural characterization and antioxidant activity of a glucan from Meripilus giganteus.
27987828	2	25	theme	methylation	422:432	arg1	analysis					434:441	methylation analysis	422:441	methylation analysis	422:441	Its structural characteristics were investigated by acid hydrolysis, methylation analysis, Smith degradation and 1D/2D NMR experiments.
27987828	4	26	theme	MGPS	584:587	arg1	backbone					572:579	The backbone	568:579	The backbone of MGPS	568:587	The backbone of MGPS was composed of two (1→3)-β-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue.
27987828	2	27	theme	Smith	444:448	arg1	degradation					450:460	Smith degradation	444:460	Smith degradation	444:460	Its structural characteristics were investigated by acid hydrolysis, methylation analysis, Smith degradation and 1D/2D NMR experiments.
27987828	1	28	theme	soluble	103:109	arg1	MGPS					119:122	MGPS	119:122	MGPS	119:122	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	1	28	theme	soluble	103:109	arg1	glucan					111:116	A new water soluble glucan	91:116	A new water soluble glucan (MGPS)	91:123	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	5	29	dep	In	966:967	arg1	vitro					969:973	vitro	969:973	vitro	969:973	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	0	30	from	giganteus	80:88	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activity of a glucan from Meripilus giganteus.
27987828	0	30	from	giganteus	80:88	arg1	activity					44:51	antioxidant activity	32:51	antioxidant activity	32:51	Structural characterization and antioxidant activity of a glucan from Meripilus giganteus.
27987828	0	30	from	giganteus	80:88	arg1	glucan					58:63	a glucan	56:63	a glucan from Meripilus giganteus	56:88	Structural characterization and antioxidant activity of a glucan from Meripilus giganteus.
27987828	2	31	theme	NMR	472:474	arg1	experiments					476:486	1D/2D NMR experiments	466:486	1D/2D NMR experiments	466:486	Its structural characteristics were investigated by acid hydrolysis, methylation analysis, Smith degradation and 1D/2D NMR experiments.
27987828	1	32	theme	dialysis	278:285	arg1	tubing					287:292	dialysis tubing cellulose membrane and sepharose 6B column chromatography	278:350	tubing	287:292	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	4	33	theme	-β-d-glucopyranosyl	760:778	arg1	residue					780:786	one (1→3)-β-d-glucopyranosyl residue	751:786	one (1→3)-β-d-glucopyranosyl residue	751:786	The backbone of MGPS was composed of two (1→3)-β-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue.
27987828	1	34	theme	fruit	185:189	arg1	bodies					191:196	the fruit bodies	181:196	the fruit bodies of Meripilus giganteus	181:219	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	0	35	theme	glucan	58:63	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activity of a glucan from Meripilus giganteus.
27987828	0	35	theme	glucan	58:63	arg1	activity					44:51	antioxidant activity	32:51	antioxidant activity	32:51	Structural characterization and antioxidant activity of a glucan from Meripilus giganteus.
27987828	3	36	contain	contain	546:552	arg2	glucose					559:565	glucose	559:565	glucose	559:565	The monosaccharide composition analysis showed that MGPS contain only glucose.
27987828	3	36	contain	contain	546:552	arg1	MGPS					541:544	MGPS	541:544	MGPS	541:544	The monosaccharide composition analysis showed that MGPS contain only glucose.
27987828	4	37	theme	β-d-glucopyranosyl	938:955	arg1	residue					957:963	terminal β-d-glucopyranosyl residue	929:963	terminal β-d-glucopyranosyl residue	929:963	The backbone of MGPS was composed of two (1→3)-β-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue.
27987828	3	38	theme	monosaccharide	493:506	arg1	analysis					520:527	The monosaccharide composition analysis	489:527	The monosaccharide composition analysis	489:527	The monosaccharide composition analysis showed that MGPS contain only glucose.
27987828	1	39	theme	cellulose	294:302	arg1	membrane					304:311	cellulose membrane	294:311	cellulose membrane	294:311	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	5	40	theme	hydroxide	1026:1034	arg1	IC50=390μg/mL					1065:1077	IC50=390μg/mL	1065:1077	IC50=390μg/mL	1065:1077	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	5	40	theme	hydroxide	1026:1034	arg1	activity					1055:1062	hydroxide radical scavenging activity	1026:1062	hydroxide radical scavenging activity (IC50=390μg/mL)	1026:1078	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	5	41	theme	radical	1036:1042	arg1	IC50=390μg/mL					1065:1077	IC50=390μg/mL	1065:1077	IC50=390μg/mL	1065:1077	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	5	41	theme	radical	1036:1042	arg1	activity					1055:1062	hydroxide radical scavenging activity	1026:1062	hydroxide radical scavenging activity (IC50=390μg/mL)	1026:1078	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	3	42	theme	composition	508:518	arg1	analysis					520:527	The monosaccharide composition analysis	489:527	The monosaccharide composition analysis	489:527	The monosaccharide composition analysis showed that MGPS contain only glucose.
27987828	5	43	theme	chelating	1152:1160	arg1	IC50=290μg/mL					1172:1184	IC50=290μg/mL	1172:1184	IC50=290μg/mL	1172:1184	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	5	43	theme	chelating	1152:1160	arg1	activity					1162:1169	ferrous ion chelating activity	1140:1169	ferrous ion chelating activity (IC50=290μg/mL)	1140:1185	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	1	44	attach	isolated	167:174	arg2	glucan					111:116	A new water soluble glucan	91:116	A new water soluble glucan (MGPS)	91:123	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	1	44	attach	isolated	167:174	arg1	bodies					191:196	the fruit bodies	181:196	the fruit bodies of Meripilus giganteus	181:219	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	1	44	attach	isolated	167:174	arg2	MGPS					119:122	MGPS	119:122	MGPS	119:122	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
27987828	5	45	theme	In	966:967	arg1	studies					987:993	In vitro antioxidant studies	966:993	In vitro antioxidant studies	966:993	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	5	46	theme	scavenging	1044:1053	arg1	IC50=390μg/mL					1065:1077	IC50=390μg/mL	1065:1077	IC50=390μg/mL	1065:1077	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	5	46	theme	scavenging	1044:1053	arg1	activity					1055:1062	hydroxide radical scavenging activity	1026:1062	hydroxide radical scavenging activity (IC50=390μg/mL)	1026:1078	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	5	47	theme	antioxidant	975:985	arg1	studies					987:993	In vitro antioxidant studies	966:993	In vitro antioxidant studies	966:993	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	2	48	theme	acid	405:408	arg1	hydrolysis					410:419	acid hydrolysis	405:419	acid hydrolysis	405:419	Its structural characteristics were investigated by acid hydrolysis, methylation analysis, Smith degradation and 1D/2D NMR experiments.
27987828	2	49	theme	structural	357:366	arg1	characteristics					368:382	Its structural characteristics	353:382	Its structural characteristics	353:382	Its structural characteristics were investigated by acid hydrolysis, methylation analysis, Smith degradation and 1D/2D NMR experiments.
27987828	2	50	theme	1D/2D	466:470	arg1	experiments					476:486	1D/2D NMR experiments	466:486	1D/2D NMR experiments	466:486	Its structural characteristics were investigated by acid hydrolysis, methylation analysis, Smith degradation and 1D/2D NMR experiments.
27987828	5	51	theme	ferrous	1140:1146	arg1	IC50=290μg/mL					1172:1184	IC50=290μg/mL	1172:1184	IC50=290μg/mL	1172:1184	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	5	51	theme	ferrous	1140:1146	arg1	activity					1162:1169	ferrous ion chelating activity	1140:1169	ferrous ion chelating activity (IC50=290μg/mL)	1140:1185	In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
27987828	1	52	theme	giganteus	211:219	arg1	bodies					191:196	the fruit bodies	181:196	the fruit bodies of Meripilus giganteus	181:219	A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography.
24507360	2	0	theme	foundation	338:347	arg1	PU					319:320	PU	319:320	PU	319:320	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	2	0	theme	foundation	338:347	arg1	polymer					349:355	the foundation polymer	334:355	the foundation polymer	334:355	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	6	1	theme	zein	1039:1042	arg1	presence					1020:1027	The presence	1016:1027	The presence of CA and zein in the nanofiber membrane	1016:1068	The presence of CA and zein in the nanofiber membrane improved its hydrophilicity, bioactivity and created a moist environment for the wound, which can accelerate wound recovery.
24507360	2	2	theme	cell	460:463	arg1	attachment					465:474	excellent cell attachment	450:474	excellent cell attachment	450:474	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	5	3	theme	enhanced	941:948	arg1	ability					965:971	enhanced blood clotting ability	941:971	enhanced blood clotting ability	941:971	PU-CA-zein-drug composite nanoscaffold showed enhanced blood clotting ability in comparison with pristine PU nanofibers.
24507360	6	4	attach	presence	1020:1027	arg2	zein					1039:1042	zein	1039:1042	zein	1039:1042	The presence of CA and zein in the nanofiber membrane improved its hydrophilicity, bioactivity and created a moist environment for the wound, which can accelerate wound recovery.
24507360	6	4	attach	presence	1020:1027	arg2	CA					1032:1033	CA	1032:1033	CA	1032:1033	The presence of CA and zein in the nanofiber membrane improved its hydrophilicity, bioactivity and created a moist environment for the wound, which can accelerate wound recovery.
24507360	6	4	attach	presence	1020:1027	arg1	membrane					1061:1068	the nanofiber membrane	1047:1068	the nanofiber membrane	1047:1068	The presence of CA and zein in the nanofiber membrane improved its hydrophilicity, bioactivity and created a moist environment for the wound, which can accelerate wound recovery.
24507360	2	5	dep	used	326:329	arg1	blended					362:368	blended	362:368	was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability	358:516	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	1	6	theme	cellulose	280:288	arg1	CA					299:300	CA	299:300	CA	299:300	In this study, an antibacterial electrospun nanofibrous scaffolds with diameters around 400-700 nm were prepared by physically blending polyurethane (PU) with two biopolymers such as cellulose acetate (CA) and zein.
24507360	1	6	theme	cellulose	280:288	arg1	acetate					290:296	cellulose acetate	280:296	cellulose acetate (CA)	280:301	In this study, an antibacterial electrospun nanofibrous scaffolds with diameters around 400-700 nm were prepared by physically blending polyurethane (PU) with two biopolymers such as cellulose acetate (CA) and zein.
24507360	2	7	theme	clotting	501:508	arg1	ability					510:516	blood clotting ability	495:516	blood clotting ability	495:516	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	3	8	theme	common	530:535	arg1	infections					546:555	common clinical infections	530:555	common clinical infections	530:555	To prevent common clinical infections, an antimicrobial agent, streptomycin sulfate was incorporated into the electrospun fibers and its antimicrobial ability against the gram negative and gram positive bacteria were examined.
24507360	2	9	theme	blood	495:499	arg1	ability					510:516	blood clotting ability	495:516	blood clotting ability	495:516	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	5	10	theme	blood	950:954	arg1	ability					965:971	enhanced blood clotting ability	941:971	enhanced blood clotting ability	941:971	PU-CA-zein-drug composite nanoscaffold showed enhanced blood clotting ability in comparison with pristine PU nanofibers.
24507360	1	11	with	scaffolds	153:161	arg1	diameters					168:176	diameters	168:176	diameters around 400-700 nm	168:194	In this study, an antibacterial electrospun nanofibrous scaffolds with diameters around 400-700 nm were prepared by physically blending polyurethane (PU) with two biopolymers such as cellulose acetate (CA) and zein.
24507360	6	12	theme	moist	1125:1129	arg1	environment					1131:1141	a moist environment	1123:1141	a moist environment for the wound, which can accelerate wound recovery	1123:1192	The presence of CA and zein in the nanofiber membrane improved its hydrophilicity, bioactivity and created a moist environment for the wound, which can accelerate wound recovery.
24507360	0	13	theme	antibacterial	12:24	arg1	composite					62:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite	0:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite	0:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite mats for wound dressing.
24507360	3	14	theme	positive	713:720	arg1	bacteria					722:729	the gram negative and gram positive bacteria	686:729	the gram negative and gram positive bacteria	686:729	To prevent common clinical infections, an antimicrobial agent, streptomycin sulfate was incorporated into the electrospun fibers and its antimicrobial ability against the gram negative and gram positive bacteria were examined.
24507360	0	15	theme	Electrospun	0:10	arg1	composite					62:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite	0:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite	0:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite mats for wound dressing.
24507360	2	16	used	used	326:329	arg2	PU					319:320	PU	319:320	PU	319:320	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	2	16	used	used	326:329	arg2	polymer					349:355	the foundation polymer	334:355	the foundation polymer	334:355	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	5	17	theme	PU-CA-zein-drug	895:909	arg1	nanoscaffold					921:932	PU-CA-zein-drug composite nanoscaffold	895:932	PU-CA-zein-drug composite nanoscaffold	895:932	PU-CA-zein-drug composite nanoscaffold showed enhanced blood clotting ability in comparison with pristine PU nanofibers.
24507360	0	18	theme	acetate-zein	49:60	arg1	composite					62:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite	0:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite	0:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite mats for wound dressing.
24507360	5	19	theme	pristine	992:999	arg1	nanofibers					1004:1013	pristine PU nanofibers	992:1013	pristine PU nanofibers	992:1013	PU-CA-zein-drug composite nanoscaffold showed enhanced blood clotting ability in comparison with pristine PU nanofibers.
24507360	2	20	theme	desirable	398:406	arg1	proliferation					477:489	proliferation	477:489	proliferation	477:489	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	2	20	theme	desirable	398:406	arg1	hydrophilicity					434:447	better hydrophilicity	427:447	better hydrophilicity	427:447	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	2	20	theme	desirable	398:406	arg1	attachment					465:474	excellent cell attachment	450:474	excellent cell attachment	450:474	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	2	20	theme	desirable	398:406	arg1	properties					408:417	desirable properties	398:417	desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability	398:516	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	2	20	theme	desirable	398:406	arg1	ability					510:516	blood clotting ability	495:516	blood clotting ability	495:516	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	1	21	theme	antibacterial	115:127	arg1	scaffolds					153:161	an antibacterial electrospun nanofibrous scaffolds	112:161	an antibacterial electrospun nanofibrous scaffolds with diameters around 400-700 nm	112:194	In this study, an antibacterial electrospun nanofibrous scaffolds with diameters around 400-700 nm were prepared by physically blending polyurethane (PU) with two biopolymers such as cellulose acetate (CA) and zein.
24507360	5	22	theme	composite	911:919	arg1	nanoscaffold					921:932	PU-CA-zein-drug composite nanoscaffold	895:932	PU-CA-zein-drug composite nanoscaffold	895:932	PU-CA-zein-drug composite nanoscaffold showed enhanced blood clotting ability in comparison with pristine PU nanofibers.
24507360	2	23	theme	excellent	450:458	arg1	attachment					465:474	excellent cell attachment	450:474	excellent cell attachment	450:474	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	5	24	theme	clotting	956:963	arg1	ability					965:971	enhanced blood clotting ability	941:971	enhanced blood clotting ability	941:971	PU-CA-zein-drug composite nanoscaffold showed enhanced blood clotting ability in comparison with pristine PU nanofibers.
24507360	6	25	from	presence	1020:1027	arg1	membrane					1061:1068	the nanofiber membrane	1047:1068	the nanofiber membrane	1047:1068	The presence of CA and zein in the nanofiber membrane improved its hydrophilicity, bioactivity and created a moist environment for the wound, which can accelerate wound recovery.
24507360	5	26	with	comparison	976:985	arg1	nanofibers					1004:1013	pristine PU nanofibers	992:1013	pristine PU nanofibers	992:1013	PU-CA-zein-drug composite nanoscaffold showed enhanced blood clotting ability in comparison with pristine PU nanofibers.
24507360	3	27	theme	streptomycin	582:593	arg1	agent					575:579	an antimicrobial agent	558:579	an antimicrobial agent	558:579	To prevent common clinical infections, an antimicrobial agent, streptomycin sulfate was incorporated into the electrospun fibers and its antimicrobial ability against the gram negative and gram positive bacteria were examined.
24507360	3	27	theme	streptomycin	582:593	arg1	sulfate					595:601	streptomycin sulfate	582:601	streptomycin sulfate	582:601	To prevent common clinical infections, an antimicrobial agent, streptomycin sulfate was incorporated into the electrospun fibers and its antimicrobial ability against the gram negative and gram positive bacteria were examined.
24507360	0	28	theme	wound	81:85	arg1	dressing					87:94	wound dressing	81:94	wound dressing	81:94	Electrospun antibacterial polyurethane-cellulose acetate-zein composite mats for wound dressing.
24507360	3	29	theme	electrospun	629:639	arg1	fibers					641:646	the electrospun fibers	625:646	the electrospun fibers	625:646	To prevent common clinical infections, an antimicrobial agent, streptomycin sulfate was incorporated into the electrospun fibers and its antimicrobial ability against the gram negative and gram positive bacteria were examined.
24507360	3	30	theme	antimicrobial	561:573	arg1	agent					575:579	an antimicrobial agent	558:579	an antimicrobial agent	558:579	To prevent common clinical infections, an antimicrobial agent, streptomycin sulfate was incorporated into the electrospun fibers and its antimicrobial ability against the gram negative and gram positive bacteria were examined.
24507360	3	30	theme	antimicrobial	561:573	arg1	sulfate					595:601	streptomycin sulfate	582:601	streptomycin sulfate	582:601	To prevent common clinical infections, an antimicrobial agent, streptomycin sulfate was incorporated into the electrospun fibers and its antimicrobial ability against the gram negative and gram positive bacteria were examined.
24507360	1	31	theme	electrospun	129:139	arg1	scaffolds					153:161	an antibacterial electrospun nanofibrous scaffolds	112:161	an antibacterial electrospun nanofibrous scaffolds with diameters around 400-700 nm	112:194	In this study, an antibacterial electrospun nanofibrous scaffolds with diameters around 400-700 nm were prepared by physically blending polyurethane (PU) with two biopolymers such as cellulose acetate (CA) and zein.
24507360	2	32	theme	better	427:432	arg1	hydrophilicity					434:447	better hydrophilicity	427:447	better hydrophilicity	427:447	Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability.
24507360	1	33	theme	nanofibrous	141:151	arg1	scaffolds					153:161	an antibacterial electrospun nanofibrous scaffolds	112:161	an antibacterial electrospun nanofibrous scaffolds with diameters around 400-700 nm	112:194	In this study, an antibacterial electrospun nanofibrous scaffolds with diameters around 400-700 nm were prepared by physically blending polyurethane (PU) with two biopolymers such as cellulose acetate (CA) and zein.
24507360	4	34	theme	PU-CA-zein-drug	800:814	arg1	attachment					864:873	attachment	864:873	attachment	864:873	The interaction between fibroblasts and the PU-CA and PU-CA-zein-drug scaffolds such as viability, proliferation, and attachment were characterized.
24507360	4	34	theme	PU-CA-zein-drug	800:814	arg1	scaffolds					816:824	the PU-CA and PU-CA-zein-drug scaffolds	786:824	the PU-CA and PU-CA-zein-drug scaffolds such as viability, proliferation, and attachment	786:873	The interaction between fibroblasts and the PU-CA and PU-CA-zein-drug scaffolds such as viability, proliferation, and attachment were characterized.
24507360	4	34	theme	PU-CA-zein-drug	800:814	arg1	viability					834:842	viability	834:842	viability	834:842	The interaction between fibroblasts and the PU-CA and PU-CA-zein-drug scaffolds such as viability, proliferation, and attachment were characterized.
24507360	4	34	theme	PU-CA-zein-drug	800:814	arg1	proliferation					845:857	proliferation	845:857	proliferation	845:857	The interaction between fibroblasts and the PU-CA and PU-CA-zein-drug scaffolds such as viability, proliferation, and attachment were characterized.
24507360	6	35	theme	wound	1179:1183	arg1	recovery					1185:1192	wound recovery	1179:1192	wound recovery	1179:1192	The presence of CA and zein in the nanofiber membrane improved its hydrophilicity, bioactivity and created a moist environment for the wound, which can accelerate wound recovery.
24507360	4	36	theme	PU-CA	790:794	arg1	attachment					864:873	attachment	864:873	attachment	864:873	The interaction between fibroblasts and the PU-CA and PU-CA-zein-drug scaffolds such as viability, proliferation, and attachment were characterized.
24507360	4	36	theme	PU-CA	790:794	arg1	scaffolds					816:824	the PU-CA and PU-CA-zein-drug scaffolds	786:824	the PU-CA and PU-CA-zein-drug scaffolds such as viability, proliferation, and attachment	786:873	The interaction between fibroblasts and the PU-CA and PU-CA-zein-drug scaffolds such as viability, proliferation, and attachment were characterized.
24507360	4	36	theme	PU-CA	790:794	arg1	viability					834:842	viability	834:842	viability	834:842	The interaction between fibroblasts and the PU-CA and PU-CA-zein-drug scaffolds such as viability, proliferation, and attachment were characterized.
24507360	4	36	theme	PU-CA	790:794	arg1	proliferation					845:857	proliferation	845:857	proliferation	845:857	The interaction between fibroblasts and the PU-CA and PU-CA-zein-drug scaffolds such as viability, proliferation, and attachment were characterized.
24507360	3	37	dep	common	530:535	arg1	clinical					537:544	clinical	537:544	clinical	537:544	To prevent common clinical infections, an antimicrobial agent, streptomycin sulfate was incorporated into the electrospun fibers and its antimicrobial ability against the gram negative and gram positive bacteria were examined.
24507360	6	38	theme	nanofiber	1051:1059	arg1	membrane					1061:1068	the nanofiber membrane	1047:1068	the nanofiber membrane	1047:1068	The presence of CA and zein in the nanofiber membrane improved its hydrophilicity, bioactivity and created a moist environment for the wound, which can accelerate wound recovery.
24507360	3	39	theme	antimicrobial	656:668	arg1	ability					670:676	its antimicrobial ability	652:676	its antimicrobial ability against the gram negative and gram positive bacteria	652:729	To prevent common clinical infections, an antimicrobial agent, streptomycin sulfate was incorporated into the electrospun fibers and its antimicrobial ability against the gram negative and gram positive bacteria were examined.
24507360	5	40	theme	PU	1001:1002	arg1	nanofibers					1004:1013	pristine PU nanofibers	992:1013	pristine PU nanofibers	992:1013	PU-CA-zein-drug composite nanoscaffold showed enhanced blood clotting ability in comparison with pristine PU nanofibers.
24507360	6	41	theme	CA	1032:1033	arg1	presence					1020:1027	The presence	1016:1027	The presence of CA and zein in the nanofiber membrane	1016:1068	The presence of CA and zein in the nanofiber membrane improved its hydrophilicity, bioactivity and created a moist environment for the wound, which can accelerate wound recovery.
24507360	3	42	theme	negative	695:702	arg1	bacteria					722:729	the gram negative and gram positive bacteria	686:729	the gram negative and gram positive bacteria	686:729	To prevent common clinical infections, an antimicrobial agent, streptomycin sulfate was incorporated into the electrospun fibers and its antimicrobial ability against the gram negative and gram positive bacteria were examined.
24507360	0	43	theme	polyurethane-cellulose	26:47	arg1	composite					62:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite	0:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite	0:70	Electrospun antibacterial polyurethane-cellulose acetate-zein composite mats for wound dressing.
28832045	4	0	theme	CDs	697:699	arg1	pathway					686:692	the pathway	682:692	the pathway of CDs	682:699	Several model plant tissues have been employed to investigate the pathway of CDs.
28832045	0	1	theme	phosphor	93:100	arg1	construction					102:113	composite phosphor construction	83:113	composite phosphor construction	83:113	Multifunctional carbon dots for highly luminescent orange-emissive cellulose based composite phosphor construction and plant tissue imaging.
28832045	5	2	theme	aqueous	780:786	arg1	solutions					788:796	aqueous solutions	780:796	aqueous solutions	780:796	Confocal analysis demonstrated that plant tissues can readily absorb CDs from aqueous solutions and bind them with cellulose-rich structures.
28832045	2	3	theme	hydrogen	346:353	arg1	binding					355:361	hydrogen binding	346:361	hydrogen binding	346:361	The reddish-orange emissive CDs can be effectively dispersed in cellulose matrices through hydrogen binding, and thus highly efficient orange-emissive CD-based phosphors were successfully obtained with a quantum yield of 44%.
28832045	1	4	theme	fast	241:244	arg1	process					246:252	a facile and fast process	228:252	a facile and fast process	228:252	A new class of carbon dot (CD) grafted cellulose hybrid phosphors has been prepared in a facile and fast process.
28832045	0	5	theme	plant	119:123	arg1	imaging					132:138	plant tissue imaging	119:138	plant tissue imaging	119:138	Multifunctional carbon dots for highly luminescent orange-emissive cellulose based composite phosphor construction and plant tissue imaging.
28832045	5	6	from	solutions	788:796	arg1	CDs					771:773	CDs	771:773	CDs from aqueous solutions	771:796	Confocal analysis demonstrated that plant tissues can readily absorb CDs from aqueous solutions and bind them with cellulose-rich structures.
28832045	5	7	theme	plant	738:742	arg1	tissues					744:750	plant tissues	738:750	plant tissues	738:750	Confocal analysis demonstrated that plant tissues can readily absorb CDs from aqueous solutions and bind them with cellulose-rich structures.
28832045	6	8	theme	CDs	905:907	arg1	exploration					890:900	the exploration	886:900	the exploration of CDs	886:907	These studies may open up new avenues for the exploration of CDs in long-wavelength emissive solid-state lighting and plant tissue imaging.
28832045	2	9	theme	emissive	274:281	arg1	CDs					283:285	The reddish-orange emissive CDs	255:285	The reddish-orange emissive CDs	255:285	The reddish-orange emissive CDs can be effectively dispersed in cellulose matrices through hydrogen binding, and thus highly efficient orange-emissive CD-based phosphors were successfully obtained with a quantum yield of 44%.
28832045	4	10	theme	model	628:632	arg1	tissues					640:646	Several model plant tissues	620:646	Several model plant tissues	620:646	Several model plant tissues have been employed to investigate the pathway of CDs.
28832045	2	11	theme	reddish-orange	259:272	arg1	CDs					283:285	The reddish-orange emissive CDs	255:285	The reddish-orange emissive CDs	255:285	The reddish-orange emissive CDs can be effectively dispersed in cellulose matrices through hydrogen binding, and thus highly efficient orange-emissive CD-based phosphors were successfully obtained with a quantum yield of 44%.
28832045	2	12	theme	orange-emissive	390:404	arg1	phosphors					415:423	highly efficient orange-emissive CD-based phosphors	373:423	highly efficient orange-emissive CD-based phosphors	373:423	The reddish-orange emissive CDs can be effectively dispersed in cellulose matrices through hydrogen binding, and thus highly efficient orange-emissive CD-based phosphors were successfully obtained with a quantum yield of 44%.
28832045	0	13	theme	tissue	125:130	arg1	imaging					132:138	plant tissue imaging	119:138	plant tissue imaging	119:138	Multifunctional carbon dots for highly luminescent orange-emissive cellulose based composite phosphor construction and plant tissue imaging.
28832045	0	14	theme	luminescent	39:49	arg1	cellulose					67:75	highly luminescent orange-emissive cellulose	32:75	highly luminescent orange-emissive cellulose	32:75	Multifunctional carbon dots for highly luminescent orange-emissive cellulose based composite phosphor construction and plant tissue imaging.
28832045	2	15	theme	efficient	380:388	arg1	phosphors					415:423	highly efficient orange-emissive CD-based phosphors	373:423	highly efficient orange-emissive CD-based phosphors	373:423	The reddish-orange emissive CDs can be effectively dispersed in cellulose matrices through hydrogen binding, and thus highly efficient orange-emissive CD-based phosphors were successfully obtained with a quantum yield of 44%.
28832045	0	16	theme	carbon	16:21	arg1	dots					23:26	Multifunctional carbon dots	0:26	Multifunctional carbon dots for highly luminescent orange-emissive cellulose	0:75	Multifunctional carbon dots for highly luminescent orange-emissive cellulose based composite phosphor construction and plant tissue imaging.
28832045	1	17	theme	grafted	172:178	arg1	phosphors					197:205	carbon dot (CD) grafted cellulose hybrid phosphors	156:205	carbon dot (CD) grafted cellulose hybrid phosphors	156:205	A new class of carbon dot (CD) grafted cellulose hybrid phosphors has been prepared in a facile and fast process.
28832045	0	18	theme	Multifunctional	0:14	arg1	dots					23:26	Multifunctional carbon dots	0:26	Multifunctional carbon dots for highly luminescent orange-emissive cellulose	0:75	Multifunctional carbon dots for highly luminescent orange-emissive cellulose based composite phosphor construction and plant tissue imaging.
28832045	3	19	theme	fluorescence	567:578	arg1	mapping					580:586	fluorescence mapping	567:586	fluorescence mapping of cellulosic plant cell walls	567:617	Moreover, the affinity of CDs for binding cellulose provides them the feasibility for fluorescence mapping of cellulosic plant cell walls.
28832045	1	20	theme	cellulose	180:188	arg1	phosphors					197:205	carbon dot (CD) grafted cellulose hybrid phosphors	156:205	carbon dot (CD) grafted cellulose hybrid phosphors	156:205	A new class of carbon dot (CD) grafted cellulose hybrid phosphors has been prepared in a facile and fast process.
28832045	5	21	theme	cellulose-rich	817:830	arg1	structures					832:841	cellulose-rich structures	817:841	cellulose-rich structures	817:841	Confocal analysis demonstrated that plant tissues can readily absorb CDs from aqueous solutions and bind them with cellulose-rich structures.
28832045	1	22	theme	hybrid	190:195	arg1	phosphors					197:205	carbon dot (CD) grafted cellulose hybrid phosphors	156:205	carbon dot (CD) grafted cellulose hybrid phosphors	156:205	A new class of carbon dot (CD) grafted cellulose hybrid phosphors has been prepared in a facile and fast process.
28832045	5	23	theme	Confocal	702:709	arg1	analysis					711:718	Confocal analysis	702:718	Confocal analysis	702:718	Confocal analysis demonstrated that plant tissues can readily absorb CDs from aqueous solutions and bind them with cellulose-rich structures.
28832045	1	24	theme	phosphors	197:205	arg1	class					147:151	A new class	141:151	A new class of carbon dot (CD) grafted cellulose hybrid phosphors	141:205	A new class of carbon dot (CD) grafted cellulose hybrid phosphors has been prepared in a facile and fast process.
28832045	2	25	theme	%	478:478	arg1	yield					467:471	a quantum yield	457:471	a quantum yield of 44%	457:478	The reddish-orange emissive CDs can be effectively dispersed in cellulose matrices through hydrogen binding, and thus highly efficient orange-emissive CD-based phosphors were successfully obtained with a quantum yield of 44%.
28832045	6	26	theme	tissue	968:973	arg1	imaging					975:981	plant tissue imaging	962:981	plant tissue imaging	962:981	These studies may open up new avenues for the exploration of CDs in long-wavelength emissive solid-state lighting and plant tissue imaging.
28832045	6	27	theme	new	870:872	arg1	avenues					874:880	new avenues	870:880	new avenues for the exploration of CDs	870:907	These studies may open up new avenues for the exploration of CDs in long-wavelength emissive solid-state lighting and plant tissue imaging.
28832045	3	28	theme	cellulosic	591:600	arg1	walls					613:617	cellulosic plant cell walls	591:617	cellulosic plant cell walls	591:617	Moreover, the affinity of CDs for binding cellulose provides them the feasibility for fluorescence mapping of cellulosic plant cell walls.
28832045	6	29	theme	plant	962:966	arg1	imaging					975:981	plant tissue imaging	962:981	plant tissue imaging	962:981	These studies may open up new avenues for the exploration of CDs in long-wavelength emissive solid-state lighting and plant tissue imaging.
28832045	3	30	theme	plant	602:606	arg1	walls					613:617	cellulosic plant cell walls	591:617	cellulosic plant cell walls	591:617	Moreover, the affinity of CDs for binding cellulose provides them the feasibility for fluorescence mapping of cellulosic plant cell walls.
28832045	2	31	theme	CD-based	406:413	arg1	phosphors					415:423	highly efficient orange-emissive CD-based phosphors	373:423	highly efficient orange-emissive CD-based phosphors	373:423	The reddish-orange emissive CDs can be effectively dispersed in cellulose matrices through hydrogen binding, and thus highly efficient orange-emissive CD-based phosphors were successfully obtained with a quantum yield of 44%.
28832045	0	32	theme	orange-emissive	51:65	arg1	cellulose					67:75	highly luminescent orange-emissive cellulose	32:75	highly luminescent orange-emissive cellulose	32:75	Multifunctional carbon dots for highly luminescent orange-emissive cellulose based composite phosphor construction and plant tissue imaging.
28832045	6	33	theme	solid-state	937:947	arg1	lighting					949:956	long-wavelength emissive solid-state lighting	912:956	long-wavelength emissive solid-state lighting	912:956	These studies may open up new avenues for the exploration of CDs in long-wavelength emissive solid-state lighting and plant tissue imaging.
28832045	1	34	theme	new	143:145	arg1	class					147:151	A new class	141:151	A new class of carbon dot (CD) grafted cellulose hybrid phosphors	141:205	A new class of carbon dot (CD) grafted cellulose hybrid phosphors has been prepared in a facile and fast process.
28832045	2	35	theme	cellulose	319:327	arg1	matrices					329:336	cellulose matrices	319:336	cellulose matrices	319:336	The reddish-orange emissive CDs can be effectively dispersed in cellulose matrices through hydrogen binding, and thus highly efficient orange-emissive CD-based phosphors were successfully obtained with a quantum yield of 44%.
28832045	0	36	theme	composite	83:91	arg1	construction					102:113	composite phosphor construction	83:113	composite phosphor construction	83:113	Multifunctional carbon dots for highly luminescent orange-emissive cellulose based composite phosphor construction and plant tissue imaging.
28832045	3	37	theme	cell	608:611	arg1	walls					613:617	cellulosic plant cell walls	591:617	cellulosic plant cell walls	591:617	Moreover, the affinity of CDs for binding cellulose provides them the feasibility for fluorescence mapping of cellulosic plant cell walls.
28832045	4	38	theme	plant	634:638	arg1	tissues					640:646	Several model plant tissues	620:646	Several model plant tissues	620:646	Several model plant tissues have been employed to investigate the pathway of CDs.
28832045	3	39	theme	CDs	507:509	arg1	affinity					495:502	the affinity	491:502	the affinity of CDs for binding cellulose	491:531	Moreover, the affinity of CDs for binding cellulose provides them the feasibility for fluorescence mapping of cellulosic plant cell walls.
28832045	4	40	theme	Several	620:626	arg1	tissues					640:646	Several model plant tissues	620:646	Several model plant tissues	620:646	Several model plant tissues have been employed to investigate the pathway of CDs.
28832045	6	41	theme	emissive	928:935	arg1	lighting					949:956	long-wavelength emissive solid-state lighting	912:956	long-wavelength emissive solid-state lighting	912:956	These studies may open up new avenues for the exploration of CDs in long-wavelength emissive solid-state lighting and plant tissue imaging.
28832045	3	42	theme	walls	613:617	arg1	mapping					580:586	fluorescence mapping	567:586	fluorescence mapping of cellulosic plant cell walls	567:617	Moreover, the affinity of CDs for binding cellulose provides them the feasibility for fluorescence mapping of cellulosic plant cell walls.
28832045	2	43	theme	quantum	459:465	arg1	yield					467:471	a quantum yield	457:471	a quantum yield of 44%	457:478	The reddish-orange emissive CDs can be effectively dispersed in cellulose matrices through hydrogen binding, and thus highly efficient orange-emissive CD-based phosphors were successfully obtained with a quantum yield of 44%.
28832045	1	44	theme	facile	230:235	arg1	process					246:252	a facile and fast process	228:252	a facile and fast process	228:252	A new class of carbon dot (CD) grafted cellulose hybrid phosphors has been prepared in a facile and fast process.
26618263	2	0	theme	pH	563:564	arg1	range					566:570	pH range	563:570	pH range	563:570	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	1	1	theme	current	153:159	arg1	work					161:164	this current work	148:164	this current work	148:164	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	4	2	theme	multiple	961:968	arg1	interactions					970:981	multiple interactions	961:981	multiple interactions between AG25 and CS-Gln-MCM	961:1009	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	1	3	theme	Fe3O4	259:263	arg1	nanoparticles					265:277	silica coated Fe3O4 nanoparticles	245:277	silica coated Fe3O4 nanoparticles (CS-Gln-MCM)	245:290	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	1	3	theme	Fe3O4	259:263	arg1	CS-Gln-MCM					280:289	CS-Gln-MCM	280:289	CS-Gln-MCM	280:289	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	0	4	theme	aqueous	60:66	arg1	solutions					68:76	aqueous solutions	60:76	aqueous solutions using glutamine	60:92	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine modified chitosan magnetic composite microspheres.
26618263	5	5	theme	aforementioned	1194:1207	arg1	pollutants					1213:1222	aforementioned two pollutants	1194:1222	aforementioned two pollutants	1194:1222	While, in weak acidic conditions, an efficient simultaneous removal is observed for different adsorption effects involved in aforementioned two pollutants.
26618263	2	6	theme	mercury	497:503	arg1	ions					505:508	mercury ions	497:508	mercury ions (Hg(2+))	497:517	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	4	7	theme	preferential	877:888	arg1	adsorption					890:899	preferential adsorption	877:899	preferential adsorption for AG25 over Hg(2+)	877:920	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	6	8	theme	rapid	1350:1354	arg1	separation					1365:1374	rapid magnetic separation	1350:1374	not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption	1257:1439	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	6	9	theme	saturated	1420:1428	arg1	adsorption					1430:1439	saturated adsorption	1420:1439	saturated adsorption	1420:1439	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	4	10	theme	Hg	824:825	arg1	mixture					839:845	Hg(2+) aqueous mixture	824:845	Hg(2+) aqueous mixture	824:845	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	2	11	theme	composite	604:612	arg1	microsphere					614:624	chitosan magnetic composite microsphere	586:624	chitosan magnetic composite microsphere (CS-MCM) without modification	586:654	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	2	11	theme	composite	604:612	arg1	CS-MCM					627:632	CS-MCM	627:632	CS-MCM	627:632	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	3	12	theme	diverse	768:774	arg1	mechanisms					787:796	diverse adsorption mechanisms	768:796	diverse adsorption mechanisms	768:796	It is due to the fact that the grafted amino acid provides a variety of additional adsorption active sites and diverse adsorption mechanisms are involved.
26618263	0	13	theme	chitosan	103:110	arg1	microspheres					131:142	chitosan magnetic composite microspheres	103:142	chitosan magnetic composite microspheres	103:142	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine modified chitosan magnetic composite microspheres.
26618263	2	14	theme	chitosan	586:593	arg1	microsphere					614:624	chitosan magnetic composite microsphere	586:624	chitosan magnetic composite microsphere (CS-MCM) without modification	586:654	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	2	14	theme	chitosan	586:593	arg1	CS-MCM					627:632	CS-MCM	627:632	CS-MCM	627:632	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	2	15	theme	magnetic	595:602	arg1	microsphere					614:624	chitosan magnetic composite microsphere	586:624	chitosan magnetic composite microsphere (CS-MCM) without modification	586:654	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	2	15	theme	magnetic	595:602	arg1	CS-MCM					627:632	CS-MCM	627:632	CS-MCM	627:632	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	6	16	theme	magnetic	1356:1363	arg1	separation					1365:1374	rapid magnetic separation	1350:1374	not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption	1257:1439	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	4	17	theme	electrostatic	1041:1053	arg1	interactions					1055:1066	electrostatic interactions	1041:1066	electrostatic interactions	1041:1066	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	2	18	theme	enhanced	415:422	arg1	efficiency					432:441	enhanced removal efficiency	415:441	enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification	415:654	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	2	19	theme	amphoteric	477:486	arg1	dye					488:490	an amphoteric dye	474:490	an amphoteric dye	474:490	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	2	19	theme	amphoteric	477:486	arg1	green					457:461	acid green 25 (AG25)	452:471	acid green 25 (AG25)	452:471	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	1	20	theme	biodegradable	373:385	arg1	materials					387:395	biodegradable materials	373:395	biodegradable materials	373:395	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	2	21	from	water	524:528	arg1	efficiency					432:441	enhanced removal efficiency	415:441	enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification	415:654	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	4	22	theme	acidic	932:937	arg1	bonding					1029:1035	hydrogen bonding	1020:1035	hydrogen bonding	1020:1035	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	4	22	theme	acidic	932:937	arg1	solutions					939:947	strong acidic solutions	925:947	strong acidic solutions	925:947	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	4	22	theme	acidic	932:937	arg1	interactions					1055:1066	electrostatic interactions	1041:1066	electrostatic interactions	1041:1066	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	7	23	contain	bears	1464:1468	arg2	potentials					1488:1497	great application potentials	1470:1497	great application potentials	1470:1497	Therefore, CS-Gln-MCM bears great application potentials in water treatment.
26618263	7	23	contain	bears	1464:1468	arg3	treatment					1508:1516	water treatment	1502:1516	water treatment	1502:1516	Therefore, CS-Gln-MCM bears great application potentials in water treatment.
26618263	7	23	contain	bears	1464:1468	arg1	CS-Gln-MCM					1453:1462	CS-Gln-MCM	1453:1462	CS-Gln-MCM	1453:1462	Therefore, CS-Gln-MCM bears great application potentials in water treatment.
26618263	6	24	theme	efficient	1391:1399	arg1	regeneration					1401:1412	efficient regeneration	1391:1412	efficient regeneration	1391:1412	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	1	25	theme	materials	387:395	arg1	which					354:358	which	354:358	which	354:358	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	1	25	theme	materials	387:395	arg1	kind					365:368	a kind	363:368	a kind of biodegradable materials	363:395	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	4	26	theme	2+	827:828	arg1	mixture					839:845	Hg(2+) aqueous mixture	824:845	Hg(2+) aqueous mixture	824:845	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	1	27	theme	magnetic	171:178	arg1	microsphere					190:200	the magnetic composite microsphere	167:200	the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM)	167:290	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	0	28	theme	Simultaneous	0:11	arg1	removal					13:19	Simultaneous removal	0:19	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine	0:92	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine modified chitosan magnetic composite microspheres.
26618263	3	29	theme	amino	696:700	arg1	acid					702:705	the grafted amino acid	684:705	the grafted amino acid	684:705	It is due to the fact that the grafted amino acid provides a variety of additional adsorption active sites and diverse adsorption mechanisms are involved.
26618263	7	30	theme	great	1470:1474	arg1	potentials					1488:1497	great application potentials	1470:1497	great application potentials	1470:1497	Therefore, CS-Gln-MCM bears great application potentials in water treatment.
26618263	4	31	theme	strong	925:930	arg1	bonding					1029:1035	hydrogen bonding	1020:1035	hydrogen bonding	1020:1035	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	4	31	theme	strong	925:930	arg1	solutions					939:947	strong acidic solutions	925:947	strong acidic solutions	925:947	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	4	31	theme	strong	925:930	arg1	interactions					1055:1066	electrostatic interactions	1041:1066	electrostatic interactions	1041:1066	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	4	32	theme	hydrogen	1020:1027	arg1	bonding					1029:1035	hydrogen bonding	1020:1035	hydrogen bonding	1020:1035	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	1	33	theme	composite	180:188	arg1	microsphere					190:200	the magnetic composite microsphere	167:200	the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM)	167:290	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	0	34	theme	acid	24:27	arg1	green					29:33	acid green 25	24:36	acid green 25	24:36	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine modified chitosan magnetic composite microspheres.
26618263	0	35	theme	composite	121:129	arg1	microspheres					131:142	chitosan magnetic composite microspheres	103:142	chitosan magnetic composite microspheres	103:142	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine modified chitosan magnetic composite microspheres.
26618263	6	36	theme	less	1322:1325	arg1	adsorption					1293:1302	adsorption	1293:1302	adsorption of each pollutant less than 20.0 min	1293:1339	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	2	37	theme	acid	452:455	arg1	green					457:461	acid green 25 (AG25)	452:471	acid green 25 (AG25)	452:471	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	2	37	theme	acid	452:455	arg1	dye					488:490	an amphoteric dye	474:490	an amphoteric dye	474:490	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	6	38	from	water	1381:1385	arg1	separation					1365:1374	rapid magnetic separation	1350:1374	not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption	1257:1439	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	6	38	from	water	1381:1385	arg1	time					1284:1287	short equilibrium time	1266:1287	not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption	1257:1439	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	0	39	theme	magnetic	112:119	arg1	microspheres					131:142	chitosan magnetic composite microspheres	103:142	chitosan magnetic composite microspheres	103:142	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine modified chitosan magnetic composite microspheres.
26618263	0	40	from	solutions	68:76	arg1	removal					13:19	Simultaneous removal	0:19	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine	0:92	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine modified chitosan magnetic composite microspheres.
26618263	5	41	theme	efficient	1106:1114	arg1	removal					1129:1135	an efficient simultaneous removal	1103:1135	an efficient simultaneous removal	1103:1135	While, in weak acidic conditions, an efficient simultaneous removal is observed for different adsorption effects involved in aforementioned two pollutants.
26618263	3	42	theme	grafted	688:694	arg1	acid					702:705	the grafted amino acid	684:705	the grafted amino acid	684:705	It is due to the fact that the grafted amino acid provides a variety of additional adsorption active sites and diverse adsorption mechanisms are involved.
26618263	6	43	theme	equilibrium	1272:1282	arg1	time					1284:1287	short equilibrium time	1266:1287	not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption	1257:1439	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	6	44	theme	short	1266:1270	arg1	time					1284:1287	short equilibrium time	1266:1287	not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption	1257:1439	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	1	45	theme	glutamine	213:221	arg1	chitosan					232:239	glutamine modified chitosan	213:239	glutamine modified chitosan	213:239	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	0	46	theme	green	29:33	arg1	removal					13:19	Simultaneous removal	0:19	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine	0:92	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine modified chitosan magnetic composite microspheres.
26618263	5	47	theme	simultaneous	1116:1127	arg1	removal					1129:1135	an efficient simultaneous removal	1103:1135	an efficient simultaneous removal	1103:1135	While, in weak acidic conditions, an efficient simultaneous removal is observed for different adsorption effects involved in aforementioned two pollutants.
26618263	2	48	dep	respective	537:546	arg1	measured					554:561	measured	554:561	measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification	554:654	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	3	49	theme	additional	729:738	arg1	sites					758:762	additional adsorption active sites	729:762	additional adsorption active sites	729:762	It is due to the fact that the grafted amino acid provides a variety of additional adsorption active sites and diverse adsorption mechanisms are involved.
26618263	1	50	theme	modified	223:230	arg1	chitosan					232:239	glutamine modified chitosan	213:239	glutamine modified chitosan	213:239	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	0	51	theme	mercury	42:48	arg1	ions					50:53	mercury ions	42:53	mercury ions	42:53	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine modified chitosan magnetic composite microspheres.
26618263	6	52	theme	pollutant	1312:1320	arg1	adsorption					1293:1302	adsorption	1293:1302	adsorption of each pollutant less than 20.0 min	1293:1339	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	7	53	theme	water	1502:1506	arg1	treatment					1508:1516	water treatment	1502:1516	water treatment	1502:1516	Therefore, CS-Gln-MCM bears great application potentials in water treatment.
26618263	3	54	theme	adsorption	776:785	arg1	mechanisms					787:796	diverse adsorption mechanisms	768:796	diverse adsorption mechanisms	768:796	It is due to the fact that the grafted amino acid provides a variety of additional adsorption active sites and diverse adsorption mechanisms are involved.
26618263	4	55	theme	modified	852:859	arg1	adsorbents					861:870	the modified adsorbents	848:870	the modified adsorbents	848:870	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	5	56	theme	weak	1079:1082	arg1	conditions					1091:1100	weak acidic conditions	1079:1100	weak acidic conditions	1079:1100	While, in weak acidic conditions, an efficient simultaneous removal is observed for different adsorption effects involved in aforementioned two pollutants.
26618263	2	57	dep	ions	505:508	arg1	2+					514:515	2+	514:515	2+	514:515	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	2	57	dep	ions	505:508	arg1	Hg					511:512	Hg	511:512	Hg(2+)	511:516	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	7	58	theme	application	1476:1486	arg1	potentials					1488:1497	great application potentials	1470:1497	great application potentials	1470:1497	Therefore, CS-Gln-MCM bears great application potentials in water treatment.
26618263	4	59	contain	bear	872:875	arg3	bonding					1029:1035	hydrogen bonding	1020:1035	hydrogen bonding	1020:1035	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	4	59	contain	bear	872:875	arg3	AG25					815:818	AG25	815:818	AG25	815:818	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	4	59	contain	bear	872:875	arg3	mixture					839:845	Hg(2+) aqueous mixture	824:845	Hg(2+) aqueous mixture	824:845	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	4	59	contain	bear	872:875	arg1	adsorbents					861:870	the modified adsorbents	848:870	the modified adsorbents	848:870	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	4	59	contain	bear	872:875	arg3	solutions					939:947	strong acidic solutions	925:947	strong acidic solutions	925:947	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	4	59	contain	bear	872:875	arg2	adsorption					890:899	preferential adsorption	877:899	preferential adsorption for AG25 over Hg(2+)	877:920	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	4	59	contain	bear	872:875	arg3	interactions					1055:1066	electrostatic interactions	1041:1066	electrostatic interactions	1041:1066	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	5	60	theme	different	1153:1161	arg1	effects					1174:1180	different adsorption effects	1153:1180	different adsorption effects involved in aforementioned two pollutants	1153:1222	While, in weak acidic conditions, an efficient simultaneous removal is observed for different adsorption effects involved in aforementioned two pollutants.
26618263	5	61	theme	acidic	1084:1089	arg1	conditions					1091:1100	weak acidic conditions	1079:1100	weak acidic conditions	1079:1100	While, in weak acidic conditions, an efficient simultaneous removal is observed for different adsorption effects involved in aforementioned two pollutants.
26618263	3	62	theme	active	751:756	arg1	sites					758:762	additional adsorption active sites	729:762	additional adsorption active sites	729:762	It is due to the fact that the grafted amino acid provides a variety of additional adsorption active sites and diverse adsorption mechanisms are involved.
26618263	4	63	theme	aqueous	831:837	arg1	mixture					839:845	Hg(2+) aqueous mixture	824:845	Hg(2+) aqueous mixture	824:845	In AG25 and Hg(2+) aqueous mixture, the modified adsorbents bear preferential adsorption for AG25 over Hg(2+) in strong acidic solutions ascribed to multiple interactions between AG25 and CS-Gln-MCM, such as hydrogen bonding and electrostatic interactions.
26618263	1	64	contain	containing	202:211	arg2	CS-Gln-MCM					280:289	CS-Gln-MCM	280:289	CS-Gln-MCM	280:289	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	1	64	contain	containing	202:211	arg2	chitosan					232:239	glutamine modified chitosan	213:239	glutamine modified chitosan	213:239	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	1	64	contain	containing	202:211	arg2	nanoparticles					265:277	silica coated Fe3O4 nanoparticles	245:277	silica coated Fe3O4 nanoparticles (CS-Gln-MCM)	245:290	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	1	64	contain	containing	202:211	arg1	microsphere					190:200	the magnetic composite microsphere	167:200	the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM)	167:290	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	6	65	from	regeneration	1401:1412	arg1	separation					1365:1374	rapid magnetic separation	1350:1374	not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption	1257:1439	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	6	65	from	regeneration	1401:1412	arg1	time					1284:1287	short equilibrium time	1266:1287	not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption	1257:1439	Besides, CS-Gln-MCM illuminates not only short equilibrium time for adsorption of each pollutant less than 20.0 min but also rapid magnetic separation from water and efficient regeneration after saturated adsorption.
26618263	2	66	from	efficiency	432:441	arg1	respective					537:546	respective	537:546	respective	537:546	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	3	67	theme	sites	758:762	arg1	sites					758:762	additional adsorption active sites	729:762	additional adsorption active sites	729:762	It is due to the fact that the grafted amino acid provides a variety of additional adsorption active sites and diverse adsorption mechanisms are involved.
26618263	3	67	theme	sites	758:762	arg1	variety					718:724	a variety	716:724	a variety of additional adsorption active sites	716:762	It is due to the fact that the grafted amino acid provides a variety of additional adsorption active sites and diverse adsorption mechanisms are involved.
26618263	2	68	theme	removal	424:430	arg1	efficiency					432:441	enhanced removal efficiency	415:441	enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification	415:654	CS-Gln-MCM shows enhanced removal efficiency for both acid green 25 (AG25), an amphoteric dye, and mercury ions (Hg(2+)) from water in the respective while measured pH range compared with chitosan magnetic composite microsphere (CS-MCM) without modification.
26618263	5	69	located	observed	1140:1147	arg2	removal					1129:1135	an efficient simultaneous removal	1103:1135	an efficient simultaneous removal	1103:1135	While, in weak acidic conditions, an efficient simultaneous removal is observed for different adsorption effects involved in aforementioned two pollutants.
26618263	5	69	located	observed	1140:1147	arg1	conditions					1091:1100	weak acidic conditions	1079:1100	weak acidic conditions	1079:1100	While, in weak acidic conditions, an efficient simultaneous removal is observed for different adsorption effects involved in aforementioned two pollutants.
26618263	1	70	theme	silica	245:250	arg1	nanoparticles					265:277	silica coated Fe3O4 nanoparticles	245:277	silica coated Fe3O4 nanoparticles (CS-Gln-MCM)	245:290	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	1	70	theme	silica	245:250	arg1	CS-Gln-MCM					280:289	CS-Gln-MCM	280:289	CS-Gln-MCM	280:289	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	0	71	theme	ions	50:53	arg1	removal					13:19	Simultaneous removal	0:19	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine	0:92	Simultaneous removal of acid green 25 and mercury ions from aqueous solutions using glutamine modified chitosan magnetic composite microspheres.
26618263	3	72	theme	adsorption	740:749	arg1	sites					758:762	additional adsorption active sites	729:762	additional adsorption active sites	729:762	It is due to the fact that the grafted amino acid provides a variety of additional adsorption active sites and diverse adsorption mechanisms are involved.
26618263	5	73	theme	adsorption	1163:1172	arg1	effects					1174:1180	different adsorption effects	1153:1180	different adsorption effects involved in aforementioned two pollutants	1153:1222	While, in weak acidic conditions, an efficient simultaneous removal is observed for different adsorption effects involved in aforementioned two pollutants.
26618263	1	74	theme	coated	252:257	arg1	nanoparticles					265:277	silica coated Fe3O4 nanoparticles	245:277	silica coated Fe3O4 nanoparticles (CS-Gln-MCM)	245:290	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26618263	1	74	theme	coated	252:257	arg1	CS-Gln-MCM					280:289	CS-Gln-MCM	280:289	CS-Gln-MCM	280:289	In this current work, the magnetic composite microsphere containing glutamine modified chitosan and silica coated Fe3O4 nanoparticles (CS-Gln-MCM) has been successfully prepared and extensively characterized, which is a kind of biodegradable materials.
26769706	1	0	theme	LMAA	263:266	arg1	process					269:275	the low moisture anhydrous ammonia (LMAA) process	227:275	the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor	227:312	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	5	1	theme	mix	768:770	arg1	hydrolysate					778:788	a mix sugar hydrolysate	766:788	a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase	766:838	The 70 % moisture CS treated at 90 °C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase.
26769706	5	1	theme	mix	768:770	arg1	MSH					791:793	MSH	791:793	MSH	791:793	The 70 % moisture CS treated at 90 °C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase.
26769706	8	2	dep	E.	1334:1335	arg1	coli					1337:1340	coli	1337:1340	coli	1337:1340	The resultant cellulose-enriched residue was hydrolyzed with cellulase to make a glucose-rich hydrolysate (GRH), which was subsequently used for succinic acid fermentation by E. coli AFP184.
26769706	8	3	theme	E.	1334:1335	arg1	AFP184					1342:1347	E. coli AFP184	1334:1347	E. coli AFP184	1334:1347	The resultant cellulose-enriched residue was hydrolyzed with cellulase to make a glucose-rich hydrolysate (GRH), which was subsequently used for succinic acid fermentation by E. coli AFP184.
26769706	8	4	theme	resultant	1163:1171	arg1	residue					1192:1198	The resultant cellulose-enriched residue	1159:1198	The resultant cellulose-enriched residue	1159:1198	The resultant cellulose-enriched residue was hydrolyzed with cellulase to make a glucose-rich hydrolysate (GRH), which was subsequently used for succinic acid fermentation by E. coli AFP184.
26769706	7	5	used	used	1094:1097	arg2	hydrolysate					1052:1062	a xylose-rich hydrolysate	1038:1062	a xylose-rich hydrolysate (XRH)	1038:1068	The 66 % moisture CS treated at 90 °C was hydrolyzed with xylanase to make a xylose-rich hydrolysate (XRH), which was subsequently used for butyric acid fermentation by Clostridium tyrobutyricum.
26769706	7	5	used	used	1094:1097	arg2	XRH					1065:1067	XRH	1065:1067	XRH	1065:1067	The 66 % moisture CS treated at 90 °C was hydrolyzed with xylanase to make a xylose-rich hydrolysate (XRH), which was subsequently used for butyric acid fermentation by Clostridium tyrobutyricum.
26769706	3	6	theme	70 	435:437	arg1	%					438:438	%	438:438	%	438:438	The 70 % moisture pretreated CS then was subjected to a storage study under non-sterile conditions for 3 months.
26769706	4	7	theme	pretreated	635:644	arg1	materials					646:654	the pretreated materials	631:654	the pretreated materials	631:654	It was found that storage time did not have significant effects on the compositions of the pretreated materials and their hydrolysis by commercial enzymes.
26769706	3	8	theme	%	438:438	arg1	CS					460:461	The 70 % moisture pretreated CS	431:461	The 70 % moisture pretreated CS	431:461	The 70 % moisture pretreated CS then was subjected to a storage study under non-sterile conditions for 3 months.
26769706	5	9	theme	sugar	772:776	arg1	hydrolysate					778:788	a mix sugar hydrolysate	766:788	a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase	766:838	The 70 % moisture CS treated at 90 °C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase.
26769706	5	9	theme	sugar	772:776	arg1	MSH					791:793	MSH	791:793	MSH	791:793	The 70 % moisture CS treated at 90 °C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase.
26769706	5	10	theme	xylanase	831:838	arg1	combination					802:812	combination	802:812	combination of cellulase and xylanase	802:838	The 70 % moisture CS treated at 90 °C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase.
26769706	0	11	theme	Pilot-Scale	74:84	arg1	Reactor					86:92	a Pilot-Scale Reactor	72:92	a Pilot-Scale Reactor	72:92	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	1	12	theme	pilot-scale	282:292	arg1	reactor					306:312	a pilot-scale ammoniation reactor	280:312	a pilot-scale ammoniation reactor	280:312	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	7	13	theme	moisture	972:979	arg1	CS					981:982	The 66 % moisture CS	963:982	The 66 % moisture CS treated at 90 °C	963:999	The 66 % moisture CS treated at 90 °C was hydrolyzed with xylanase to make a xylose-rich hydrolysate (XRH), which was subsequently used for butyric acid fermentation by Clostridium tyrobutyricum.
26769706	8	14	theme	cellulose-enriched	1173:1190	arg1	residue					1192:1198	The resultant cellulose-enriched residue	1159:1198	The resultant cellulose-enriched residue	1159:1198	The resultant cellulose-enriched residue was hydrolyzed with cellulase to make a glucose-rich hydrolysate (GRH), which was subsequently used for succinic acid fermentation by E. coli AFP184.
26769706	6	15	theme	ethanol	916:922	arg1	fermentation					924:935	ethanol fermentation	916:935	ethanol fermentation	916:935	The MSH was used to prepare a corn mash at 9.5 wt% solid then subjected to ethanol fermentation by Escherichia coli KO11.
26769706	1	16	theme	Corn	154:157	arg1	stover					159:164	Corn stover	154:164	Corn stover (CS) adjusted to 50, 66, and 70 % moisture	154:207	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	1	16	theme	Corn	154:157	arg1	CS					167:168	CS	167:168	CS	167:168	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	1	17	theme	ammoniation	294:304	arg1	reactor					306:312	a pilot-scale ammoniation reactor	280:312	a pilot-scale ammoniation reactor	280:312	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	8	18	used	used	1295:1298	arg2	GRH					1266:1268	GRH	1266:1268	GRH	1266:1268	The resultant cellulose-enriched residue was hydrolyzed with cellulase to make a glucose-rich hydrolysate (GRH), which was subsequently used for succinic acid fermentation by E. coli AFP184.
26769706	8	18	used	used	1295:1298	arg2	hydrolysate					1253:1263	a glucose-rich hydrolysate	1238:1263	a glucose-rich hydrolysate (GRH)	1238:1269	The resultant cellulose-enriched residue was hydrolyzed with cellulase to make a glucose-rich hydrolysate (GRH), which was subsequently used for succinic acid fermentation by E. coli AFP184.
26769706	5	19	used	used	742:745	arg2	CS					718:719	The 70 % moisture CS	700:719	The 70 % moisture CS treated at 90 °C	700:736	The 70 % moisture CS treated at 90 °C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase.
26769706	4	20	theme	materials	646:654	arg1	hydrolysis					666:675	their hydrolysis	660:675	their hydrolysis by commercial enzymes	660:697	It was found that storage time did not have significant effects on the compositions of the pretreated materials and their hydrolysis by commercial enzymes.
26769706	4	20	theme	materials	646:654	arg1	compositions					615:626	the compositions	611:626	the compositions of the pretreated materials	611:654	It was found that storage time did not have significant effects on the compositions of the pretreated materials and their hydrolysis by commercial enzymes.
26769706	6	21	theme	solid	892:896	arg1	%					890:890	9.5 wt% solid	884:896	9.5 wt% solid then subjected to ethanol fermentation by Escherichia coli KO11	884:960	The MSH was used to prepare a corn mash at 9.5 wt% solid then subjected to ethanol fermentation by Escherichia coli KO11.
26769706	0	22	theme	Stover	21:26	arg1	Pretreatment					0:11	Pretreatment	0:11	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.	0:152	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	3	23	theme	moisture	440:447	arg1	CS					460:461	The 70 % moisture pretreated CS	431:461	The 70 % moisture pretreated CS	431:461	The 70 % moisture pretreated CS then was subjected to a storage study under non-sterile conditions for 3 months.
26769706	8	24	theme	glucose-rich	1240:1251	arg1	GRH					1266:1268	GRH	1266:1268	GRH	1266:1268	The resultant cellulose-enriched residue was hydrolyzed with cellulase to make a glucose-rich hydrolysate (GRH), which was subsequently used for succinic acid fermentation by E. coli AFP184.
26769706	8	24	theme	glucose-rich	1240:1251	arg1	hydrolysate					1253:1263	a glucose-rich hydrolysate	1238:1263	a glucose-rich hydrolysate (GRH)	1238:1269	The resultant cellulose-enriched residue was hydrolyzed with cellulase to make a glucose-rich hydrolysate (GRH), which was subsequently used for succinic acid fermentation by E. coli AFP184.
26769706	2	25	theme	%	341:341	arg1	CS					352:353	the 70 % moisture CS	334:353	the 70 % moisture CS	334:353	After ammoniation, the 70 % moisture CS was treated at 90 and 100 °C whereas the others were treated at 90 °C only.
26769706	2	26	theme	moisture	343:350	arg1	CS					352:353	the 70 % moisture CS	334:353	the 70 % moisture CS	334:353	After ammoniation, the 70 % moisture CS was treated at 90 and 100 °C whereas the others were treated at 90 °C only.
26769706	3	27	theme	pretreated	449:458	arg1	CS					460:461	The 70 % moisture pretreated CS	431:461	The 70 % moisture pretreated CS	431:461	The 70 % moisture pretreated CS then was subjected to a storage study under non-sterile conditions for 3 months.
26769706	2	28	theme	70 	338:340	arg1	%					341:341	%	341:341	%	341:341	After ammoniation, the 70 % moisture CS was treated at 90 and 100 °C whereas the others were treated at 90 °C only.
26769706	0	29	theme	Low	31:33	arg1	LMAA					63:66	LMAA	63:66	LMAA	63:66	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	0	29	theme	Low	31:33	arg1	Ammonia					54:60	Low Moisture Anhydrous Ammonia	31:60	Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals	31:151	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	5	30	theme	cellulase	817:825	arg1	combination					802:812	combination	802:812	combination of cellulase and xylanase	802:838	The 70 % moisture CS treated at 90 °C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase.
26769706	0	31	theme	Fuel	115:118	arg1	Ethanol					120:126	Fuel Ethanol	115:126	Fuel Ethanol	115:126	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	4	32	theme	commercial	680:689	arg1	enzymes					691:697	commercial enzymes	680:697	commercial enzymes	680:697	It was found that storage time did not have significant effects on the compositions of the pretreated materials and their hydrolysis by commercial enzymes.
26769706	1	33	from	process	269:275	arg1	reactor					306:312	a pilot-scale ammoniation reactor	280:312	a pilot-scale ammoniation reactor	280:312	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	1	34	theme	low	231:233	arg1	process					269:275	the low moisture anhydrous ammonia (LMAA) process	227:275	the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor	227:312	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	5	35	theme	70 	704:706	arg1	%					707:707	%	707:707	%	707:707	The 70 % moisture CS treated at 90 °C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase.
26769706	7	36	theme	butyric	1103:1109	arg1	acid					1111:1114	butyric acid	1103:1114	butyric acid fermentation	1103:1127	The 66 % moisture CS treated at 90 °C was hydrolyzed with xylanase to make a xylose-rich hydrolysate (XRH), which was subsequently used for butyric acid fermentation by Clostridium tyrobutyricum.
26769706	0	37	theme	Anhydrous	44:52	arg1	LMAA					63:66	LMAA	63:66	LMAA	63:66	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	0	37	theme	Anhydrous	44:52	arg1	Ammonia					54:60	Low Moisture Anhydrous Ammonia	31:60	Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals	31:151	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	1	38	theme	moisture	235:242	arg1	process					269:275	the low moisture anhydrous ammonia (LMAA) process	227:275	the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor	227:312	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	5	39	theme	%	707:707	arg1	CS					718:719	The 70 % moisture CS	700:719	The 70 % moisture CS treated at 90 °C	700:736	The 70 % moisture CS treated at 90 °C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase.
26769706	0	40	from	Ammonia	54:60	arg1	Bioconversion					98:110	Bioconversion	98:110	Bioconversion to Fuel Ethanol and Industrial Chemicals	98:151	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	0	40	from	Ammonia	54:60	arg1	Reactor					86:92	a Pilot-Scale Reactor	72:92	a Pilot-Scale Reactor	72:92	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	6	41	dep	Escherichia	940:950	arg1	coli					952:955	coli	952:955	coli	952:955	The MSH was used to prepare a corn mash at 9.5 wt% solid then subjected to ethanol fermentation by Escherichia coli KO11.
26769706	4	42	contain	have	583:586	arg2	effects					600:606	significant effects	588:606	significant effects	588:606	It was found that storage time did not have significant effects on the compositions of the pretreated materials and their hydrolysis by commercial enzymes.
26769706	4	42	contain	have	583:586	arg1	time					570:573	storage time	562:573	storage time	562:573	It was found that storage time did not have significant effects on the compositions of the pretreated materials and their hydrolysis by commercial enzymes.
26769706	4	43	theme	significant	588:598	arg1	effects					600:606	significant effects	588:606	significant effects	588:606	It was found that storage time did not have significant effects on the compositions of the pretreated materials and their hydrolysis by commercial enzymes.
26769706	0	44	theme	Moisture	35:42	arg1	LMAA					63:66	LMAA	63:66	LMAA	63:66	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	0	44	theme	Moisture	35:42	arg1	Ammonia					54:60	Low Moisture Anhydrous Ammonia	31:60	Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals	31:151	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	1	45	theme	anhydrous	244:252	arg1	process					269:275	the low moisture anhydrous ammonia (LMAA) process	227:275	the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor	227:312	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	1	46	dep	%	198:198	arg1	66					187:188	66	187:188	66	187:188	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	1	46	dep	%	198:198	arg1	70 					195:197	70 	195:197	70 	195:197	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	5	47	theme	hydrolysate	778:788	arg1	preparation					751:761	preparation	751:761	preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase	751:838	The 70 % moisture CS treated at 90 °C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase.
26769706	1	48	theme	ammonia	254:260	arg1	process					269:275	the low moisture anhydrous ammonia (LMAA) process	227:275	the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor	227:312	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	1	49	dep	moisture	200:207	arg1	%					198:198	%	198:198	%	198:198	Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor.
26769706	7	50	theme	%	970:970	arg1	CS					981:982	The 66 % moisture CS	963:982	The 66 % moisture CS treated at 90 °C	963:999	The 66 % moisture CS treated at 90 °C was hydrolyzed with xylanase to make a xylose-rich hydrolysate (XRH), which was subsequently used for butyric acid fermentation by Clostridium tyrobutyricum.
26769706	6	51	used	used	853:856	arg2	MSH					845:847	The MSH	841:847	The MSH	841:847	The MSH was used to prepare a corn mash at 9.5 wt% solid then subjected to ethanol fermentation by Escherichia coli KO11.
26769706	6	52	theme	corn	871:874	arg1	mash					876:879	a corn mash	869:879	a corn mash	869:879	The MSH was used to prepare a corn mash at 9.5 wt% solid then subjected to ethanol fermentation by Escherichia coli KO11.
26769706	0	53	theme	Industrial	132:141	arg1	Chemicals					143:151	Industrial Chemicals	132:151	Industrial Chemicals	132:151	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	8	54	theme	acid	1313:1316	arg1	fermentation					1318:1329	succinic acid fermentation	1304:1329	succinic acid fermentation	1304:1329	The resultant cellulose-enriched residue was hydrolyzed with cellulase to make a glucose-rich hydrolysate (GRH), which was subsequently used for succinic acid fermentation by E. coli AFP184.
26769706	3	55	theme	non-sterile	507:517	arg1	conditions					519:528	non-sterile conditions	507:528	non-sterile conditions for 3 months	507:541	The 70 % moisture pretreated CS then was subjected to a storage study under non-sterile conditions for 3 months.
26769706	7	56	theme	xylose-rich	1040:1050	arg1	XRH					1065:1067	XRH	1065:1067	XRH	1065:1067	The 66 % moisture CS treated at 90 °C was hydrolyzed with xylanase to make a xylose-rich hydrolysate (XRH), which was subsequently used for butyric acid fermentation by Clostridium tyrobutyricum.
26769706	7	56	theme	xylose-rich	1040:1050	arg1	hydrolysate					1052:1062	a xylose-rich hydrolysate	1038:1062	a xylose-rich hydrolysate (XRH)	1038:1068	The 66 % moisture CS treated at 90 °C was hydrolyzed with xylanase to make a xylose-rich hydrolysate (XRH), which was subsequently used for butyric acid fermentation by Clostridium tyrobutyricum.
26769706	8	57	theme	succinic	1304:1311	arg1	fermentation					1318:1329	succinic acid fermentation	1304:1329	succinic acid fermentation	1304:1329	The resultant cellulose-enriched residue was hydrolyzed with cellulase to make a glucose-rich hydrolysate (GRH), which was subsequently used for succinic acid fermentation by E. coli AFP184.
26769706	3	58	theme	storage	487:493	arg1	study					495:499	a storage study	485:499	a storage study	485:499	The 70 % moisture pretreated CS then was subjected to a storage study under non-sterile conditions for 3 months.
26769706	5	59	theme	moisture	709:716	arg1	CS					718:719	The 70 % moisture CS	700:719	The 70 % moisture CS treated at 90 °C	700:736	The 70 % moisture CS treated at 90 °C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase.
26769706	6	60	theme	Escherichia	940:950	arg1	KO11					957:960	Escherichia coli KO11	940:960	Escherichia coli KO11	940:960	The MSH was used to prepare a corn mash at 9.5 wt% solid then subjected to ethanol fermentation by Escherichia coli KO11.
26769706	7	61	theme	acid	1111:1114	arg1	fermentation					1116:1127	butyric acid fermentation	1103:1127	butyric acid fermentation	1103:1127	The 66 % moisture CS treated at 90 °C was hydrolyzed with xylanase to make a xylose-rich hydrolysate (XRH), which was subsequently used for butyric acid fermentation by Clostridium tyrobutyricum.
26769706	7	62	theme	66 	967:969	arg1	%					970:970	%	970:970	%	970:970	The 66 % moisture CS treated at 90 °C was hydrolyzed with xylanase to make a xylose-rich hydrolysate (XRH), which was subsequently used for butyric acid fermentation by Clostridium tyrobutyricum.
26769706	0	63	theme	Corn	16:19	arg1	Stover					21:26	Corn Stover	16:26	Corn Stover	16:26	Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals.
26769706	4	64	theme	storage	562:568	arg1	time					570:573	storage time	562:573	storage time	562:573	It was found that storage time did not have significant effects on the compositions of the pretreated materials and their hydrolysis by commercial enzymes.
29030182	7	0	theme	SC5314	1079:1084	arg1	strain					1086:1091	the Candida albicans SC5314 strain	1058:1091	the Candida albicans SC5314 strain	1058:1091	Moreover, at this concentration, dextran breaks up to 70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains.
29030182	0	1	theme	functional	62:71	arg1	characteristics					73:87	its In vitro functional characteristics	49:87	its In vitro functional characteristics	49:87	Biosynthesis of dextran by Weissella confusa and its In vitro functional characteristics.
29030182	1	2	from	influence	131:139	arg1	biosynthesis					204:215	the exopolysaccharides (EPS) biosynthesis	175:215	the exopolysaccharides (EPS) biosynthesis	175:215	The aim of this study was to monitor the influence of the fermentation conditions on the exopolysaccharides (EPS) biosynthesis.
29030182	4	3	theme	NMR	624:626	arg1	analysis					628:635	NMR analysis	624:635	NMR analysis	624:635	Using FTIR and NMR analysis, it was demonstrated that the obtained EPS is a dextran.
29030182	8	4	theme	controlled	1243:1252	arg1	release					1254:1260	controlled release	1243:1260	controlled release of drugs in pharmaceutical industry	1243:1296	Due to their characteristics, these EPS are suitable as hydrophilic matrix for controlled release of drugs in pharmaceutical industry.
29030182	6	5	contain	have	906:909	arg1	concentration					858:870	A concentration	856:870	A concentration up to 3mg/mL of dextran	856:894	A concentration up to 3mg/mL of dextran proved to have no cytotoxic effect on normal human dermal fibroblasts (NHDF).
29030182	6	5	contain	have	906:909	arg2	effect					924:929	no cytotoxic effect	911:929	no cytotoxic effect	911:929	A concentration up to 3mg/mL of dextran proved to have no cytotoxic effect on normal human dermal fibroblasts (NHDF).
29030182	7	6	dep	biofilms	1039:1046	arg1	has					1098:1100	has	1098:1100	has no antimicrobial activity against standard bacterial strains	1098:1161	Moreover, at this concentration, dextran breaks up to 70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains.
29030182	7	6	dep	biofilms	1039:1046	arg1	formed					1048:1053	formed	1048:1053	formed by the Candida albicans SC5314 strain	1048:1091	Moreover, at this concentration, dextran breaks up to 70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains.
29030182	4	7	theme	obtained	667:674	arg1	EPS					676:678	the obtained EPS	663:678	the obtained EPS	663:678	Using FTIR and NMR analysis, it was demonstrated that the obtained EPS is a dextran.
29030182	4	7	theme	obtained	667:674	arg1	dextran					685:691	a dextran	683:691	a dextran	683:691	Using FTIR and NMR analysis, it was demonstrated that the obtained EPS is a dextran.
29030182	5	8	with	structure	734:742	arg1	purity					754:759	high purity	749:759	high purity	749:759	The thermal analysis revealed a dextran structure with high purity while GPC analysis depicted more fractions, which is normal for a biological obtained polymer.
29030182	3	9	theme	UHT	499:501	arg1	milk					503:506	UHT milk	499:506	UHT milk	499:506	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	5	10	theme	obtained	838:845	arg1	polymer					847:853	a biological obtained polymer	825:853	a biological obtained polymer	825:853	The thermal analysis revealed a dextran structure with high purity while GPC analysis depicted more fractions, which is normal for a biological obtained polymer.
29030182	3	11	theme	MRS	439:441	arg1	medium					443:448	MRS medium	439:448	MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk	439:506	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	2	12	theme	Weissella	368:376	arg1	confusa					378:384	Weissella confusa	368:384	Weissella confusa	368:384	For this, different culture media compositions were tested on an isolated lactic acid bacteria (LAB) strain, identified by 16S rDNA sequence as being Weissella confusa.
29030182	8	13	theme	hydrophilic	1220:1230	arg1	matrix					1232:1237	hydrophilic matrix	1220:1237	hydrophilic matrix for controlled release of drugs in pharmaceutical industry	1220:1296	Due to their characteristics, these EPS are suitable as hydrophilic matrix for controlled release of drugs in pharmaceutical industry.
29030182	5	14	theme	more	789:792	arg1	fractions					794:802	more fractions	789:802	more fractions	789:802	The thermal analysis revealed a dextran structure with high purity while GPC analysis depicted more fractions, which is normal for a biological obtained polymer.
29030182	6	15	theme	dermal	947:952	arg1	NHDF					967:970	NHDF	967:970	NHDF	967:970	A concentration up to 3mg/mL of dextran proved to have no cytotoxic effect on normal human dermal fibroblasts (NHDF).
29030182	6	15	theme	dermal	947:952	arg1	fibroblasts					954:964	normal human dermal fibroblasts	934:964	normal human dermal fibroblasts (NHDF)	934:971	A concentration up to 3mg/mL of dextran proved to have no cytotoxic effect on normal human dermal fibroblasts (NHDF).
29030182	6	16	theme	dextran	888:894	arg1	3mg/mL					878:883	3mg/mL	878:883	3mg/mL of dextran	878:894	A concentration up to 3mg/mL of dextran proved to have no cytotoxic effect on normal human dermal fibroblasts (NHDF).
29030182	3	17	from	culture	428:434	arg1	medium					443:448	MRS medium	439:448	MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk	439:506	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	2	18	theme	media	246:250	arg1	compositions					252:263	different culture media compositions	228:263	different culture media compositions	228:263	For this, different culture media compositions were tested on an isolated lactic acid bacteria (LAB) strain, identified by 16S rDNA sequence as being Weissella confusa.
29030182	1	19	theme	fermentation	148:159	arg1	conditions					161:170	the fermentation conditions	144:170	the fermentation conditions	144:170	The aim of this study was to monitor the influence of the fermentation conditions on the exopolysaccharides (EPS) biosynthesis.
29030182	7	20	theme	biofilms	1039:1046	arg1	%					1030:1030	70%	1028:1030	70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains	1028:1161	Moreover, at this concentration, dextran breaks up to 70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains.
29030182	7	20	theme	biofilms	1039:1046	arg1	biofilms					1039:1046	the biofilms	1035:1046	the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains	1035:1161	Moreover, at this concentration, dextran breaks up to 70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains.
29030182	2	21	theme	lactic	292:297	arg1	LAB					314:316	LAB	314:316	LAB	314:316	For this, different culture media compositions were tested on an isolated lactic acid bacteria (LAB) strain, identified by 16S rDNA sequence as being Weissella confusa.
29030182	2	21	theme	lactic	292:297	arg1	bacteria					304:311	lactic acid bacteria	292:311	an isolated lactic acid bacteria (LAB) strain	280:324	For this, different culture media compositions were tested on an isolated lactic acid bacteria (LAB) strain, identified by 16S rDNA sequence as being Weissella confusa.
29030182	7	22	theme	standard	1136:1143	arg1	strains					1155:1161	standard bacterial strains	1136:1161	standard bacterial strains	1136:1161	Moreover, at this concentration, dextran breaks up to 70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains.
29030182	1	23	theme	conditions	161:170	arg1	influence					131:139	the influence	127:139	the influence of the fermentation conditions on the exopolysaccharides (EPS) biosynthesis	127:215	The aim of this study was to monitor the influence of the fermentation conditions on the exopolysaccharides (EPS) biosynthesis.
29030182	5	24	theme	dextran	726:732	arg1	structure					734:742	a dextran structure	724:742	a dextran structure with high purity	724:759	The thermal analysis revealed a dextran structure with high purity while GPC analysis depicted more fractions, which is normal for a biological obtained polymer.
29030182	6	25	dep	3mg/mL	878:883	arg1	up					872:873	up	872:873	up	872:873	A concentration up to 3mg/mL of dextran proved to have no cytotoxic effect on normal human dermal fibroblasts (NHDF).
29030182	2	26	theme	isolated	283:290	arg1	strain					319:324	an isolated lactic acid bacteria (LAB) strain	280:324	an isolated lactic acid bacteria (LAB) strain	280:324	For this, different culture media compositions were tested on an isolated lactic acid bacteria (LAB) strain, identified by 16S rDNA sequence as being Weissella confusa.
29030182	8	27	theme	drugs	1265:1269	arg1	release					1254:1260	controlled release	1243:1260	controlled release of drugs in pharmaceutical industry	1243:1296	Due to their characteristics, these EPS are suitable as hydrophilic matrix for controlled release of drugs in pharmaceutical industry.
29030182	3	28	theme	bacterial	411:419	arg1	culture					428:434	this bacterial strain culture	406:434	this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk	406:506	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	0	29	theme	dextran	16:22	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis of dextran by Weissella confusa and its In vitro functional characteristics.	0:88	Biosynthesis of dextran by Weissella confusa and its In vitro functional characteristics.
29030182	0	30	dep	In	53:54	arg1	vitro					56:60	vitro	56:60	vitro	56:60	Biosynthesis of dextran by Weissella confusa and its In vitro functional characteristics.
29030182	1	31	theme	exopolysaccharides	179:196	arg1	biosynthesis					204:215	the exopolysaccharides (EPS) biosynthesis	175:215	the exopolysaccharides (EPS) biosynthesis	175:215	The aim of this study was to monitor the influence of the fermentation conditions on the exopolysaccharides (EPS) biosynthesis.
29030182	7	32	theme	antimicrobial	1105:1117	arg1	activity					1119:1126	no antimicrobial activity	1102:1126	no antimicrobial activity against standard bacterial strains	1102:1161	Moreover, at this concentration, dextran breaks up to 70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains.
29030182	3	33	theme	freeze-dried	534:545	arg1	EPS					547:549	freeze-dried EPS	534:549	freeze-dried EPS	534:549	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	6	34	theme	human	941:945	arg1	NHDF					967:970	NHDF	967:970	NHDF	967:970	A concentration up to 3mg/mL of dextran proved to have no cytotoxic effect on normal human dermal fibroblasts (NHDF).
29030182	6	34	theme	human	941:945	arg1	fibroblasts					954:964	normal human dermal fibroblasts	934:964	normal human dermal fibroblasts (NHDF)	934:971	A concentration up to 3mg/mL of dextran proved to have no cytotoxic effect on normal human dermal fibroblasts (NHDF).
29030182	0	35	theme	Weissella	27:35	arg1	confusa					37:43	Weissella confusa	27:43	Weissella confusa	27:43	Biosynthesis of dextran by Weissella confusa and its In vitro functional characteristics.
29030182	7	36	from	concentration	992:1004	arg1	breaks					1015:1020	breaks	1015:1020	breaks	1015:1020	Moreover, at this concentration, dextran breaks up to 70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains.
29030182	5	37	theme	thermal	698:704	arg1	analysis					706:713	The thermal analysis	694:713	The thermal analysis	694:713	The thermal analysis revealed a dextran structure with high purity while GPC analysis depicted more fractions, which is normal for a biological obtained polymer.
29030182	3	38	theme	EPS	547:549	arg1	25.2g/L					523:529	25.2g/L	523:529	25.2g/L of freeze-dried EPS	523:549	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	5	39	theme	GPC	767:769	arg1	analysis					771:778	GPC analysis	767:778	GPC analysis	767:778	The thermal analysis revealed a dextran structure with high purity while GPC analysis depicted more fractions, which is normal for a biological obtained polymer.
29030182	1	40	theme	EPS	199:201	arg1	biosynthesis					204:215	the exopolysaccharides (EPS) biosynthesis	175:215	the exopolysaccharides (EPS) biosynthesis	175:215	The aim of this study was to monitor the influence of the fermentation conditions on the exopolysaccharides (EPS) biosynthesis.
29030182	7	41	theme	Candida	1062:1068	arg1	albicans					1070:1077	Candida albicans	1062:1077	the Candida albicans SC5314 strain	1058:1091	Moreover, at this concentration, dextran breaks up to 70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains.
29030182	6	42	theme	normal	934:939	arg1	NHDF					967:970	NHDF	967:970	NHDF	967:970	A concentration up to 3mg/mL of dextran proved to have no cytotoxic effect on normal human dermal fibroblasts (NHDF).
29030182	6	42	theme	normal	934:939	arg1	fibroblasts					954:964	normal human dermal fibroblasts	934:964	normal human dermal fibroblasts (NHDF)	934:971	A concentration up to 3mg/mL of dextran proved to have no cytotoxic effect on normal human dermal fibroblasts (NHDF).
29030182	8	43	theme	pharmaceutical	1274:1287	arg1	industry					1289:1296	pharmaceutical industry	1274:1296	pharmaceutical industry	1274:1296	Due to their characteristics, these EPS are suitable as hydrophilic matrix for controlled release of drugs in pharmaceutical industry.
29030182	3	44	theme	strain	421:426	arg1	culture					428:434	this bacterial strain culture	406:434	this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk	406:506	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	7	45	theme	bacterial	1145:1153	arg1	strains					1155:1161	standard bacterial strains	1136:1161	standard bacterial strains	1136:1161	Moreover, at this concentration, dextran breaks up to 70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains.
29030182	1	46	theme	study	106:110	arg1	aim					94:96	The aim	90:96	The aim of this study	90:110	The aim of this study was to monitor the influence of the fermentation conditions on the exopolysaccharides (EPS) biosynthesis.
29030182	3	47	theme	static	555:560	arg1	conditions					562:571	static conditions	555:571	static conditions	555:571	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	2	48	theme	culture	238:244	arg1	compositions					252:263	different culture media compositions	228:263	different culture media compositions	228:263	For this, different culture media compositions were tested on an isolated lactic acid bacteria (LAB) strain, identified by 16S rDNA sequence as being Weissella confusa.
29030182	2	49	theme	bacteria	304:311	arg1	strain					319:324	an isolated lactic acid bacteria (LAB) strain	280:324	an isolated lactic acid bacteria (LAB) strain	280:324	For this, different culture media compositions were tested on an isolated lactic acid bacteria (LAB) strain, identified by 16S rDNA sequence as being Weissella confusa.
29030182	3	50	dep	medium	443:448	arg1	dissolved					486:494	dissolved	486:494	dissolved in UHT milk	486:506	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	3	50	dep	medium	443:448	arg1	supplemented					450:461	supplemented	450:461	supplemented with 80g/L sucrose	450:480	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	3	51	theme	fermentative	587:598	arg1	process					600:606	fermentative process	587:606	fermentative process	587:606	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	2	52	theme	different	228:236	arg1	compositions					252:263	different culture media compositions	228:263	different culture media compositions	228:263	For this, different culture media compositions were tested on an isolated lactic acid bacteria (LAB) strain, identified by 16S rDNA sequence as being Weissella confusa.
29030182	2	53	theme	acid	299:302	arg1	LAB					314:316	LAB	314:316	LAB	314:316	For this, different culture media compositions were tested on an isolated lactic acid bacteria (LAB) strain, identified by 16S rDNA sequence as being Weissella confusa.
29030182	2	53	theme	acid	299:302	arg1	bacteria					304:311	lactic acid bacteria	292:311	an isolated lactic acid bacteria (LAB) strain	280:324	For this, different culture media compositions were tested on an isolated lactic acid bacteria (LAB) strain, identified by 16S rDNA sequence as being Weissella confusa.
29030182	5	54	theme	biological	827:836	arg1	polymer					847:853	a biological obtained polymer	825:853	a biological obtained polymer	825:853	The thermal analysis revealed a dextran structure with high purity while GPC analysis depicted more fractions, which is normal for a biological obtained polymer.
29030182	3	55	theme	process	600:606	arg1	48h					580:582	48h	580:582	48h of fermentative process	580:606	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	5	56	theme	high	749:752	arg1	purity					754:759	high purity	749:759	high purity	749:759	The thermal analysis revealed a dextran structure with high purity while GPC analysis depicted more fractions, which is normal for a biological obtained polymer.
29030182	3	57	theme	80g/L	468:472	arg1	sucrose					474:480	80g/L sucrose	468:480	80g/L sucrose	468:480	It was proved that this bacterial strain culture in MRS medium supplemented with 80g/L sucrose and dissolved in UHT milk produced up to 25.2g/L of freeze-dried EPS, in static conditions, after 48h of fermentative process.
29030182	2	58	theme	rDNA	345:348	arg1	sequence					350:357	rDNA sequence	345:357	rDNA sequence	345:357	For this, different culture media compositions were tested on an isolated lactic acid bacteria (LAB) strain, identified by 16S rDNA sequence as being Weissella confusa.
29030182	6	59	theme	cytotoxic	914:922	arg1	effect					924:929	no cytotoxic effect	911:929	no cytotoxic effect	911:929	A concentration up to 3mg/mL of dextran proved to have no cytotoxic effect on normal human dermal fibroblasts (NHDF).
29030182	0	60	theme	In	53:54	arg1	characteristics					73:87	its In vitro functional characteristics	49:87	its In vitro functional characteristics	49:87	Biosynthesis of dextran by Weissella confusa and its In vitro functional characteristics.
29030182	8	61	from	release	1254:1260	arg1	industry					1289:1296	pharmaceutical industry	1274:1296	pharmaceutical industry	1274:1296	Due to their characteristics, these EPS are suitable as hydrophilic matrix for controlled release of drugs in pharmaceutical industry.
29030182	7	62	theme	albicans	1070:1077	arg1	strain					1086:1091	the Candida albicans SC5314 strain	1058:1091	the Candida albicans SC5314 strain	1058:1091	Moreover, at this concentration, dextran breaks up to 70% of the biofilms formed by the Candida albicans SC5314 strain, and has no antimicrobial activity against standard bacterial strains.
28832493	6	0	dep	showed	707:712	arg1	increased					869:877	increased	869:877	increased Firmicutes/Bacteroidetes ratio	869:908	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	6	0	dep	showed	707:712	arg1	reduced					914:920	reduced	914:920	reduced SCFA concentrations (t test, p < 0.05)	914:959	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	1	1	from	loss	159:162	arg1	mice					167:170	mice	167:170	mice	167:170	Agavins consumption has led to accelerated body weight loss in mice.
28832493	1	2	theme	accelerated	135:145	arg1	loss					159:162	accelerated body weight loss	135:162	accelerated body weight loss in mice	135:170	Agavins consumption has led to accelerated body weight loss in mice.
28832493	6	3	dep	associated	763:772	arg1	tree					829:832	PD whole tree	820:832	PD whole tree	820:832	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	5	4	theme	SCFA	650:653	arg1	concentrations					655:668	microbiota and SCFA concentrations	635:668	microbiota and SCFA concentrations	635:668	Cecal contents were collected before and after supplementation to determine microbiota and SCFA concentrations.
28832493	4	5	theme	standard	433:440	arg1	diet					442:445	standard diet	433:445	standard diet alone (HF-ST10)	433:461	Secondly, overweight mice were shifted to standard diet alone (HF-ST10) or supplemented with agavins (HF-ST + A10) or oligofructose (HF-ST + O10), for five more weeks.
28832493	4	5	theme	standard	433:440	arg1	HF-ST10					454:460	HF-ST10	454:460	HF-ST10	454:460	Secondly, overweight mice were shifted to standard diet alone (HF-ST10) or supplemented with agavins (HF-ST + A10) or oligofructose (HF-ST + O10), for five more weeks.
28832493	6	6	dep	tree	829:832	arg1	test					852:855	non-parametric t test	835:855	non-parametric t test	835:855	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	6	6	dep	tree	829:832	arg1	<					860:860	p < 0.05	858:865	p < 0.05	858:865	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	0	7	with	Association	69:79	arg1	Loss					98:101	Body Weight Loss	86:101	Body Weight Loss	86:101	Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss.
28832493	1	8	theme	body	147:150	arg1	loss					159:162	accelerated body weight loss	135:162	accelerated body weight loss in mice	135:170	Agavins consumption has led to accelerated body weight loss in mice.
28832493	2	9	from	loss	284:287	arg1	mice					303:306	overweight mice	292:306	overweight mice	292:306	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	6	10	theme	t	850:850	arg1	test					852:855	non-parametric t test	835:855	non-parametric t test	835:855	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	0	11	theme	Body	86:89	arg1	Loss					98:101	Body Weight Loss	86:101	Body Weight Loss	86:101	Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss.
28832493	7	12	theme	p	1247:1247	arg1	levels					1239:1244	higher SCFA levels	1227:1244	higher SCFA levels (p < 0.1)	1227:1254	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	7	12	theme	p	1247:1247	arg1	<					1249:1249	p < 0.1	1247:1253	p < 0.1	1247:1253	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	7	13	theme	microbiota	1136:1145	arg1	restoration					1147:1157	partial microbiota restoration	1128:1157	partial microbiota restoration	1128:1157	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	6	14	theme	first	685:689	arg1	phase					691:695	first phase	685:695	first phase	685:695	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	7	15	theme	Firmicutes/Bacteroides	1186:1207	arg1	ratio					1209:1213	the Firmicutes/Bacteroides ratio	1182:1213	the Firmicutes/Bacteroides ratio	1182:1213	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	6	16	theme	non-parametric	835:848	arg1	test					852:855	non-parametric t test	835:855	non-parametric t test	835:855	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	7	17	theme	increased	1018:1026	arg1	diversity					1032:1040	increased its diversity	1018:1040	increased its diversity	1018:1040	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	7	18	theme	SCFA	1234:1237	arg1	levels					1239:1244	higher SCFA levels	1227:1244	higher SCFA levels (p < 0.1)	1227:1254	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	7	18	theme	SCFA	1234:1237	arg1	<					1249:1249	p < 0.1	1247:1253	p < 0.1	1247:1253	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	7	19	theme	SCFA	1047:1050	arg1	levels					1052:1057	SCFA levels	1047:1057	SCFA levels	1047:1057	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	9	20	theme	diversity	1515:1523	arg1	extent					1505:1510	the extent	1501:1510	the extent of diversity	1501:1523	In conclusion, agavins or oligofructose modulated cecal microbiota composition, reduced the extent of diversity, and increased SCFA.
28832493	7	21	theme	levels	1239:1244	arg1	normalization					1165:1177	normalization	1165:1177	normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1)	1165:1254	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	6	22	theme	SCFA	922:925	arg1	concentrations					927:940	SCFA concentrations	922:940	SCFA concentrations (t test, p < 0.05)	922:959	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	6	22	theme	SCFA	922:925	arg1	test					945:948	t test	943:948	t test	943:948	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	7	23	theme	ratio	1209:1213	arg1	normalization					1165:1177	normalization	1165:1177	normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1)	1165:1254	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	2	24	from	changes	193:199	arg1	microbiota					210:219	cecal microbiota	204:219	cecal microbiota	204:219	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	2	24	from	changes	193:199	arg1	SCFA					250:253	SCFA	250:253	SCFA	250:253	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	2	24	from	changes	193:199	arg1	acids					243:247	short-chain fatty acids	225:247	short-chain fatty acids (SCFA)	225:254	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	1	25	theme	weight	152:157	arg1	loss					159:162	accelerated body weight loss	135:162	accelerated body weight loss in mice	135:170	Agavins consumption has led to accelerated body weight loss in mice.
28832493	0	26	theme	Weight	91:96	arg1	Loss					98:101	Body Weight Loss	86:101	Body Weight Loss	86:101	Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss.
28832493	6	27	theme	weight	745:750	arg1	increase					728:735	a significant increase	714:735	a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05),	714:867	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	7	28	theme	higher	1227:1232	arg1	levels					1239:1244	higher SCFA levels	1227:1244	higher SCFA levels (p < 0.1)	1227:1254	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	7	28	theme	higher	1227:1232	arg1	<					1249:1249	p < 0.1	1247:1253	p < 0.1	1247:1253	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	3	29	theme	standard	337:344	arg1	ST5					347:349	standard (ST5) or high-fat (HF5) diet	337:373	ST5	347:349	Firstly, mice were fed with standard (ST5) or high-fat (HF5) diet for five weeks.
28832493	4	30	theme	overweight	401:410	arg1	mice					412:415	overweight mice	401:415	overweight mice	401:415	Secondly, overweight mice were shifted to standard diet alone (HF-ST10) or supplemented with agavins (HF-ST + A10) or oligofructose (HF-ST + O10), for five more weeks.
28832493	9	31	theme	cecal	1463:1467	arg1	composition					1480:1490	cecal microbiota composition	1463:1490	cecal microbiota composition	1463:1490	In conclusion, agavins or oligofructose modulated cecal microbiota composition, reduced the extent of diversity, and increased SCFA.
28832493	2	32	theme	weight	277:282	arg1	loss					284:287	body weight loss	272:287	body weight loss in overweight mice	272:306	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	3	33	theme	HF5	365:367	arg1	diet					370:373	standard (ST5) or high-fat (HF5) diet	337:373	diet	370:373	Firstly, mice were fed with standard (ST5) or high-fat (HF5) diet for five weeks.
28832493	0	34	theme	Microbiota	18:27	arg1	Modulation					0:9	Modulation	0:9	Modulation of Gut Microbiota of Overweight Mice by Agavins	0:57	Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss.
28832493	0	34	theme	Microbiota	18:27	arg1	Association					69:79	Their Association	63:79	Their Association with Body Weight Loss	63:101	Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss.
28832493	6	35	theme	whole	823:827	arg1	tree					829:832	PD whole tree	820:832	PD whole tree	820:832	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	2	36	theme	body	272:275	arg1	loss					284:287	body weight loss	272:287	body weight loss in overweight mice	272:306	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	6	37	theme	phase	691:695	arg1	end					678:680	the end	674:680	the end of first phase	674:695	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	6	38	theme	Firmicutes/Bacteroidetes	879:902	arg1	ratio					904:908	Firmicutes/Bacteroidetes ratio	879:908	Firmicutes/Bacteroidetes ratio	879:908	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	0	39	theme	Gut	14:16	arg1	Microbiota					18:27	Gut Microbiota	14:27	Gut Microbiota of Overweight Mice	14:46	Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss.
28832493	6	40	theme	PD	820:821	arg1	tree					829:832	PD whole tree	820:832	PD whole tree	820:832	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	8	41	theme	LDA	1323:1325	arg1	>					1327:1327	LDA > 3.0	1323:1331	LDA > 3.0	1323:1331	Moreover, agavins noticeably enriched Klebsiella and Citrobacter (LDA > 3.0); this enrichment has not been reported previously under a prebiotic treatment.
28832493	8	41	theme	LDA	1323:1325	arg1	Klebsiella					1295:1304	Klebsiella	1295:1304	Klebsiella	1295:1304	Moreover, agavins noticeably enriched Klebsiella and Citrobacter (LDA > 3.0); this enrichment has not been reported previously under a prebiotic treatment.
28832493	7	42	theme	partial	1128:1134	arg1	restoration					1147:1157	partial microbiota restoration	1128:1157	partial microbiota restoration	1128:1157	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	0	43	theme	Overweight	32:41	arg1	Mice					43:46	Overweight Mice	32:46	Overweight Mice	32:46	Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss.
28832493	3	44	theme	high-fat	355:362	arg1	diet					370:373	standard (ST5) or high-fat (HF5) diet	337:373	diet	370:373	Firstly, mice were fed with standard (ST5) or high-fat (HF5) diet for five weeks.
28832493	6	45	theme	diversity	809:817	arg1	reduction					779:787	reduction	779:787	reduction of cecal microbiota diversity	779:817	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	7	46	theme	diet	968:971	arg1	shifting					973:980	diet shifting	968:980	diet shifting	968:980	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	6	47	theme	significant	716:726	arg1	increase					728:735	a significant increase	714:735	a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05),	714:867	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	2	48	theme	cecal	204:208	arg1	microbiota					210:219	cecal microbiota	204:219	cecal microbiota	204:219	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	6	49	theme	microbiota	798:807	arg1	diversity					809:817	cecal microbiota diversity	792:817	cecal microbiota diversity	792:817	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	9	50	theme	microbiota	1469:1478	arg1	composition					1480:1490	cecal microbiota composition	1463:1490	cecal microbiota composition	1463:1490	In conclusion, agavins or oligofructose modulated cecal microbiota composition, reduced the extent of diversity, and increased SCFA.
28832493	4	51	dep	agavins	484:490	arg1	HF-ST					493:497	HF-ST	493:497	HF-ST	493:497	Secondly, overweight mice were shifted to standard diet alone (HF-ST10) or supplemented with agavins (HF-ST + A10) or oligofructose (HF-ST + O10), for five more weeks.
28832493	4	51	dep	agavins	484:490	arg1	A10					501:503	A10	501:503	A10	501:503	Secondly, overweight mice were shifted to standard diet alone (HF-ST10) or supplemented with agavins (HF-ST + A10) or oligofructose (HF-ST + O10), for five more weeks.
28832493	6	52	dep	test	945:948	arg1	<					953:953	p < 0.05	951:958	p < 0.05	951:958	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	0	53	theme	Mice	43:46	arg1	Microbiota					18:27	Gut Microbiota	14:27	Gut Microbiota of Overweight Mice	14:46	Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss.
28832493	6	54	theme	cecal	792:796	arg1	diversity					809:817	cecal microbiota diversity	792:817	cecal microbiota diversity	792:817	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	10	55	theme	new	1637:1639	arg1	probiotics					1641:1650	new probiotics	1637:1650	new probiotics	1637:1650	Furthermore, identification of bacteria enriched by agavins opens opportunities to explore new probiotics.
28832493	4	56	dep	oligofructose	509:521	arg1	O10					532:534	O10	532:534	O10	532:534	Secondly, overweight mice were shifted to standard diet alone (HF-ST10) or supplemented with agavins (HF-ST + A10) or oligofructose (HF-ST + O10), for five more weeks.
28832493	4	56	dep	oligofructose	509:521	arg1	HF-ST					524:528	HF-ST	524:528	HF-ST	524:528	Secondly, overweight mice were shifted to standard diet alone (HF-ST10) or supplemented with agavins (HF-ST + A10) or oligofructose (HF-ST + O10), for five more weeks.
28832493	1	57	theme	Agavins	104:110	arg1	consumption					112:122	Agavins consumption	104:122	Agavins consumption	104:122	Agavins consumption has led to accelerated body weight loss in mice.
28832493	8	58	theme	prebiotic	1392:1400	arg1	treatment					1402:1410	a prebiotic treatment	1390:1410	a prebiotic treatment	1390:1410	Moreover, agavins noticeably enriched Klebsiella and Citrobacter (LDA > 3.0); this enrichment has not been reported previously under a prebiotic treatment.
28832493	6	59	theme	body	740:743	arg1	weight					745:750	body weight	740:750	body weight	740:750	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	0	60	with	Modulation	0:9	arg1	Loss					98:101	Body Weight Loss	86:101	Body Weight Loss	86:101	Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss.
28832493	2	61	theme	overweight	292:301	arg1	mice					303:306	overweight mice	292:306	overweight mice	292:306	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	5	62	theme	Cecal	559:563	arg1	contents					565:572	Cecal contents	559:572	Cecal contents	559:572	Cecal contents were collected before and after supplementation to determine microbiota and SCFA concentrations.
28832493	6	63	theme	HF5	698:700	arg1	mice					702:705	HF5 mice	698:705	HF5 mice	698:705	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	2	64	theme	fatty	237:241	arg1	SCFA					250:253	SCFA	250:253	SCFA	250:253	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	2	64	theme	fatty	237:241	arg1	acids					243:247	short-chain fatty acids	225:247	short-chain fatty acids (SCFA)	225:254	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	10	65	theme	bacteria	1577:1584	arg1	identification					1559:1572	identification	1559:1572	identification of bacteria enriched by agavins	1559:1604	Furthermore, identification of bacteria enriched by agavins opens opportunities to explore new probiotics.
28832493	6	66	theme	p	951:951	arg1	<					953:953	p < 0.05	951:958	p < 0.05	951:958	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	2	67	theme	short-chain	225:235	arg1	SCFA					250:253	SCFA	250:253	SCFA	250:253	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	2	67	theme	short-chain	225:235	arg1	acids					243:247	short-chain fatty acids	225:247	short-chain fatty acids (SCFA)	225:254	We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice.
28832493	7	68	dep	agavins	1068:1074	arg1	A10					1085:1087	A10	1085:1087	A10	1085:1087	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	7	68	dep	agavins	1068:1074	arg1	HF-ST					1077:1081	HF-ST	1077:1081	HF-ST	1077:1081	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	6	69	theme	p	858:858	arg1	<					860:860	p < 0.05	858:865	p < 0.05	858:865	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	5	70	theme	microbiota	635:644	arg1	concentrations					655:668	microbiota and SCFA concentrations	635:668	microbiota and SCFA concentrations	635:668	Cecal contents were collected before and after supplementation to determine microbiota and SCFA concentrations.
28832493	7	71	dep	oligofructose	1093:1105	arg1	HF-ST					1108:1112	HF-ST	1108:1112	HF-ST	1108:1112	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	7	71	dep	oligofructose	1093:1105	arg1	O10					1116:1118	O10	1116:1118	O10	1116:1118	After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1).
28832493	6	72	theme	t	943:943	arg1	concentrations					927:940	SCFA concentrations	922:940	SCFA concentrations (t test, p < 0.05)	922:959	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28832493	6	72	theme	t	943:943	arg1	test					945:948	t test	943:948	t test	943:948	At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05).
28821105	4	0	theme	XOS	474:476	arg1	yield					478:482	The highest XOS yield	462:482	The highest XOS yield (62% of the feedstock xylan)	462:511	The highest XOS yield (62% of the feedstock xylan) was obtained at 190°C and 5min of holding time.
28821105	1	1	theme	pruning	124:130	arg1	residues					132:139	Hazelnut shell (HS), husk and pruning residues	94:139	Hazelnut shell (HS), husk and pruning residues	94:139	Hazelnut shell (HS), husk and pruning residues were characterized and evaluated for xylooligosaccharides (XOS) production by autohydrolysis.
28821105	7	2	theme	severity	873:880	arg1	factor					882:887	severity factor	873:887	severity factor (log Ro) values	873:903	The concentrations of the products in the autohydrolysis liquors followed specific trends with changing severity factor (log Ro) values.
28821105	7	2	theme	severity	873:880	arg1	Ro					894:895	log Ro	890:895	log Ro	890:895	The concentrations of the products in the autohydrolysis liquors followed specific trends with changing severity factor (log Ro) values.
28821105	8	3	theme	cellulose	961:969	arg1	contents					982:989	the cellulose and lignin contents	957:989	the cellulose and lignin contents in the remaining solids	957:1013	Solubilization of xylan in the treatments enhanced the cellulose and lignin contents in the remaining solids.
28821105	6	4	theme	treatment	749:757	arg1	severity					759:766	treatment severity	749:766	treatment severity	749:766	Xylose, acetic acid and furfural concentrations increased with treatment severity.
28821105	8	5	theme	lignin	975:980	arg1	contents					982:989	the cellulose and lignin contents	957:989	the cellulose and lignin contents in the remaining solids	957:1013	Solubilization of xylan in the treatments enhanced the cellulose and lignin contents in the remaining solids.
28821105	5	6	theme	holding	591:597	arg1	time					599:602	holding time	591:602	holding time	591:602	At this temperature, 30min of holding time was required to maximize the percentage of XOS with low degree of polymerization.
28821105	2	7	theme	xylan	270:274	arg1	xylan					270:274	xylan	270:274	xylan	270:274	HS contained the highest amount of xylan and yielded more XOS compared to other hazelnut residues.
28821105	2	7	theme	xylan	270:274	arg1	amount					260:265	the highest amount	248:265	the highest amount of xylan	248:274	HS contained the highest amount of xylan and yielded more XOS compared to other hazelnut residues.
28821105	0	8	dep	Corylus	66:72	arg1	L.					83:84	Corylus avellana L.	66:84	Corylus avellana L.	66:84	Production of xylooligosaccharides by autohydrolysis of hazelnut (Corylus avellana L.) shell.
28821105	2	9	contain	contained	238:246	arg1	HS					235:236	HS	235:236	HS	235:236	HS contained the highest amount of xylan and yielded more XOS compared to other hazelnut residues.
28821105	2	9	contain	contained	238:246	arg2	xylan					270:274	xylan	270:274	xylan	270:274	HS contained the highest amount of xylan and yielded more XOS compared to other hazelnut residues.
28821105	2	9	contain	contained	238:246	arg2	amount					260:265	the highest amount	248:265	the highest amount of xylan	248:274	HS contained the highest amount of xylan and yielded more XOS compared to other hazelnut residues.
28821105	3	10	theme	temperature	338:348	arg1	time					362:365	The temperature and holding time	334:365	time	362:365	The temperature and holding time of HS autohydrolysis greatly influenced the composition of the liquor and the remaining solid.
28821105	5	11	theme	time	599:602	arg1	30min					582:586	30min	582:586	30min of holding time	582:602	At this temperature, 30min of holding time was required to maximize the percentage of XOS with low degree of polymerization.
28821105	7	12	theme	products	795:802	arg1	concentrations					773:786	The concentrations	769:786	The concentrations of the products in the autohydrolysis liquors	769:832	The concentrations of the products in the autohydrolysis liquors followed specific trends with changing severity factor (log Ro) values.
28821105	8	13	theme	xylan	924:928	arg1	Solubilization					906:919	Solubilization	906:919	Solubilization of xylan in the treatments	906:946	Solubilization of xylan in the treatments enhanced the cellulose and lignin contents in the remaining solids.
28821105	7	14	from	liquors	826:832	arg1	concentrations					773:786	The concentrations	769:786	The concentrations of the products in the autohydrolysis liquors	769:832	The concentrations of the products in the autohydrolysis liquors followed specific trends with changing severity factor (log Ro) values.
28821105	0	15	theme	hazelnut	56:63	arg1	Production					0:9	Production	0:9	Production of xylooligosaccharides by autohydrolysis of hazelnut (Corylus avellana L.)	0:85	Production of xylooligosaccharides by autohydrolysis of hazelnut (Corylus avellana L.) shell.
28821105	4	16	theme	highest	466:472	arg1	yield					478:482	The highest XOS yield	462:482	The highest XOS yield (62% of the feedstock xylan)	462:511	The highest XOS yield (62% of the feedstock xylan) was obtained at 190°C and 5min of holding time.
28821105	6	17	theme	furfural	710:717	arg1	concentrations					719:732	Xylose, acetic acid and furfural concentrations	686:732	Xylose, acetic acid and furfural concentrations	686:732	Xylose, acetic acid and furfural concentrations increased with treatment severity.
28821105	0	18	dep	shell	87:91	arg1	Production					0:9	Production	0:9	Production of xylooligosaccharides by autohydrolysis of hazelnut (Corylus avellana L.)	0:85	Production of xylooligosaccharides by autohydrolysis of hazelnut (Corylus avellana L.) shell.
28821105	4	19	dep	yield	478:482	arg1	%					487:487	62%	485:487	62% of the feedstock xylan	485:510	The highest XOS yield (62% of the feedstock xylan) was obtained at 190°C and 5min of holding time.
28821105	4	19	dep	yield	478:482	arg1	xylan					506:510	the feedstock xylan	492:510	the feedstock xylan	492:510	The highest XOS yield (62% of the feedstock xylan) was obtained at 190°C and 5min of holding time.
28821105	7	20	theme	log	890:892	arg1	factor					882:887	severity factor	873:887	severity factor (log Ro) values	873:903	The concentrations of the products in the autohydrolysis liquors followed specific trends with changing severity factor (log Ro) values.
28821105	7	20	theme	log	890:892	arg1	Ro					894:895	log Ro	890:895	log Ro	890:895	The concentrations of the products in the autohydrolysis liquors followed specific trends with changing severity factor (log Ro) values.
28821105	8	21	from	contents	982:989	arg1	solids					1008:1013	the remaining solids	994:1013	the remaining solids	994:1013	Solubilization of xylan in the treatments enhanced the cellulose and lignin contents in the remaining solids.
28821105	8	22	theme	remaining	998:1006	arg1	solids					1008:1013	the remaining solids	994:1013	the remaining solids	994:1013	Solubilization of xylan in the treatments enhanced the cellulose and lignin contents in the remaining solids.
28821105	6	23	theme	acid	701:704	arg1	concentrations					719:732	Xylose, acetic acid and furfural concentrations	686:732	Xylose, acetic acid and furfural concentrations	686:732	Xylose, acetic acid and furfural concentrations increased with treatment severity.
28821105	2	24	theme	more	288:291	arg1	XOS					293:295	more XOS	288:295	more XOS	288:295	HS contained the highest amount of xylan and yielded more XOS compared to other hazelnut residues.
28821105	4	25	theme	xylan	506:510	arg1	%					487:487	62%	485:487	62% of the feedstock xylan	485:510	The highest XOS yield (62% of the feedstock xylan) was obtained at 190°C and 5min of holding time.
28821105	4	25	theme	xylan	506:510	arg1	xylan					506:510	the feedstock xylan	492:510	the feedstock xylan	492:510	The highest XOS yield (62% of the feedstock xylan) was obtained at 190°C and 5min of holding time.
28821105	6	26	theme	acetic	694:699	arg1	acid					701:704	acetic acid	694:704	acetic acid	694:704	Xylose, acetic acid and furfural concentrations increased with treatment severity.
28821105	1	27	theme	Hazelnut	94:101	arg1	HS					110:111	HS	110:111	HS	110:111	Hazelnut shell (HS), husk and pruning residues were characterized and evaluated for xylooligosaccharides (XOS) production by autohydrolysis.
28821105	1	27	theme	Hazelnut	94:101	arg1	shell					103:107	Hazelnut shell	94:107	Hazelnut shell (HS)	94:112	Hazelnut shell (HS), husk and pruning residues were characterized and evaluated for xylooligosaccharides (XOS) production by autohydrolysis.
28821105	3	28	theme	holding	354:360	arg1	time					362:365	The temperature and holding time	334:365	time	362:365	The temperature and holding time of HS autohydrolysis greatly influenced the composition of the liquor and the remaining solid.
28821105	1	29	theme	shell	103:107	arg1	residues					132:139	Hazelnut shell (HS), husk and pruning residues	94:139	Hazelnut shell (HS), husk and pruning residues	94:139	Hazelnut shell (HS), husk and pruning residues were characterized and evaluated for xylooligosaccharides (XOS) production by autohydrolysis.
28821105	1	30	theme	xylooligosaccharides	178:197	arg1	production					205:214	xylooligosaccharides (XOS) production	178:214	xylooligosaccharides (XOS) production by autohydrolysis	178:232	Hazelnut shell (HS), husk and pruning residues were characterized and evaluated for xylooligosaccharides (XOS) production by autohydrolysis.
28821105	3	31	theme	liquor	430:435	arg1	composition					411:421	the composition	407:421	the composition of the liquor and the remaining solid	407:459	The temperature and holding time of HS autohydrolysis greatly influenced the composition of the liquor and the remaining solid.
28821105	5	32	theme	XOS	647:649	arg1	percentage					633:642	the percentage	629:642	the percentage of XOS	629:649	At this temperature, 30min of holding time was required to maximize the percentage of XOS with low degree of polymerization.
28821105	5	33	theme	low	656:658	arg1	degree					660:665	low degree	656:665	low degree of polymerization	656:683	At this temperature, 30min of holding time was required to maximize the percentage of XOS with low degree of polymerization.
28821105	3	34	theme	HS	370:371	arg1	autohydrolysis					373:386	HS autohydrolysis	370:386	HS autohydrolysis	370:386	The temperature and holding time of HS autohydrolysis greatly influenced the composition of the liquor and the remaining solid.
28821105	6	35	theme	Xylose	686:691	arg1	concentrations					719:732	Xylose, acetic acid and furfural concentrations	686:732	Xylose, acetic acid and furfural concentrations	686:732	Xylose, acetic acid and furfural concentrations increased with treatment severity.
28821105	2	36	theme	hazelnut	315:322	arg1	residues					324:331	other hazelnut residues	309:331	other hazelnut residues	309:331	HS contained the highest amount of xylan and yielded more XOS compared to other hazelnut residues.
28821105	3	37	theme	autohydrolysis	373:386	arg1	time					362:365	The temperature and holding time	334:365	time	362:365	The temperature and holding time of HS autohydrolysis greatly influenced the composition of the liquor and the remaining solid.
28821105	4	38	theme	feedstock	496:504	arg1	xylan					506:510	the feedstock xylan	492:510	the feedstock xylan	492:510	The highest XOS yield (62% of the feedstock xylan) was obtained at 190°C and 5min of holding time.
28821105	7	39	from	concentrations	773:786	arg1	liquors					826:832	the autohydrolysis liquors	807:832	the autohydrolysis liquors	807:832	The concentrations of the products in the autohydrolysis liquors followed specific trends with changing severity factor (log Ro) values.
28821105	2	40	theme	other	309:313	arg1	residues					324:331	other hazelnut residues	309:331	other hazelnut residues	309:331	HS contained the highest amount of xylan and yielded more XOS compared to other hazelnut residues.
28821105	7	41	theme	factor	882:887	arg1	values					898:903	severity factor (log Ro) values	873:903	severity factor (log Ro) values	873:903	The concentrations of the products in the autohydrolysis liquors followed specific trends with changing severity factor (log Ro) values.
28821105	3	42	theme	solid	455:459	arg1	composition					411:421	the composition	407:421	the composition of the liquor and the remaining solid	407:459	The temperature and holding time of HS autohydrolysis greatly influenced the composition of the liquor and the remaining solid.
28821105	4	43	theme	holding	547:553	arg1	time					555:558	holding time	547:558	holding time	547:558	The highest XOS yield (62% of the feedstock xylan) was obtained at 190°C and 5min of holding time.
28821105	2	44	theme	highest	252:258	arg1	xylan					270:274	xylan	270:274	xylan	270:274	HS contained the highest amount of xylan and yielded more XOS compared to other hazelnut residues.
28821105	2	44	theme	highest	252:258	arg1	amount					260:265	the highest amount	248:265	the highest amount of xylan	248:274	HS contained the highest amount of xylan and yielded more XOS compared to other hazelnut residues.
28821105	8	45	from	Solubilization	906:919	arg1	treatments					937:946	the treatments	933:946	the treatments	933:946	Solubilization of xylan in the treatments enhanced the cellulose and lignin contents in the remaining solids.
28821105	7	46	theme	specific	843:850	arg1	trends					852:857	specific trends	843:857	specific trends	843:857	The concentrations of the products in the autohydrolysis liquors followed specific trends with changing severity factor (log Ro) values.
28821105	5	47	theme	polymerization	670:683	arg1	degree					660:665	low degree	656:665	low degree of polymerization	656:683	At this temperature, 30min of holding time was required to maximize the percentage of XOS with low degree of polymerization.
28821105	7	48	theme	autohydrolysis	811:824	arg1	liquors					826:832	the autohydrolysis liquors	807:832	the autohydrolysis liquors	807:832	The concentrations of the products in the autohydrolysis liquors followed specific trends with changing severity factor (log Ro) values.
28821105	7	49	from	products	795:802	arg1	liquors					826:832	the autohydrolysis liquors	807:832	the autohydrolysis liquors	807:832	The concentrations of the products in the autohydrolysis liquors followed specific trends with changing severity factor (log Ro) values.
28821105	1	50	theme	husk	115:118	arg1	residues					132:139	Hazelnut shell (HS), husk and pruning residues	94:139	Hazelnut shell (HS), husk and pruning residues	94:139	Hazelnut shell (HS), husk and pruning residues were characterized and evaluated for xylooligosaccharides (XOS) production by autohydrolysis.
28821105	0	51	theme	xylooligosaccharides	14:33	arg1	Production					0:9	Production	0:9	Production of xylooligosaccharides by autohydrolysis of hazelnut (Corylus avellana L.)	0:85	Production of xylooligosaccharides by autohydrolysis of hazelnut (Corylus avellana L.) shell.
28821105	4	52	theme	time	555:558	arg1	5min					539:542	5min	539:542	5min	539:542	The highest XOS yield (62% of the feedstock xylan) was obtained at 190°C and 5min of holding time.
28821105	4	52	theme	time	555:558	arg1	190°C					529:533	190°C	529:533	190°C	529:533	The highest XOS yield (62% of the feedstock xylan) was obtained at 190°C and 5min of holding time.
26650723	11	0	theme	synthetic	1379:1387	arg1	materials					1389:1397	synthetic materials	1379:1397	synthetic materials	1379:1397	CONCLUSIONS The composite scaffold could present substantial benefits compared to synthetic materials.
26650723	7	1	dep	RESULTS	807:813	arg1	enabled					848:854	enabled	848:854	enabled the shape of a gutta-percha point to be mimicked with great accuracy and reproducibility (P = 0.31)	848:954	RESULTS The fabrication method developed enabled the shape of a gutta-percha point to be mimicked with great accuracy and reproducibility (P = 0.31).
26650723	5	2	theme	tissue	689:694	arg1	compaction					696:705	tissue compaction	689:705	tissue compaction	689:705	Elastic modulus, tissue compaction and cell differentiation were assessed.
26650723	2	3	theme	collagen/alginate	353:369	arg1	scaffold					381:388	A collagen/alginate composite scaffold	351:388	METHODOLOGY A collagen/alginate composite scaffold	339:388	METHODOLOGY A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone.
26650723	10	4	theme	significant	1209:1219	arg1	contraction					1221:1231	no significant contraction	1206:1231	no significant contraction	1206:1231	The construct showed no significant contraction after 8 days, preserving its shape and tip diameter (P = 0.58).
26650723	12	5	theme	root	1457:1460	arg1	canal					1462:1466	the root canal	1453:1466	the root canal	1453:1466	It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.
26650723	7	6	theme	point	884:888	arg1	shape					860:864	the shape	856:864	the shape of a gutta-percha point	856:888	RESULTS The fabrication method developed enabled the shape of a gutta-percha point to be mimicked with great accuracy and reproducibility (P = 0.31).
26650723	1	7	theme	mesenchymal	249:259	arg1	cells					266:270	mesenchymal stem cells	249:270	mesenchymal stem cells	249:270	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	1	8	used	used	295:298	arg2	material					329:336	a regenerative endodontic material	303:336	a regenerative endodontic material	303:336	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	1	8	used	used	295:298	arg2	geometry					168:175	the geometry	164:175	the geometry of a gutta-percha point with precision	164:214	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	1	9	theme	stem	261:264	arg1	cells					266:270	mesenchymal stem cells	249:270	mesenchymal stem cells	249:270	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	8	10	theme	Stem	957:960	arg1	cells					962:966	Stem cells	957:966	Stem cells seeded into this composite scaffold	957:1002	Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001).
26650723	8	11	dep	spread	1017:1022	arg1	proliferate					1037:1047	proliferate	1037:1047	proliferate	1037:1047	Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001).
26650723	8	11	dep	spread	1017:1022	arg1	survive					1025:1031	survive	1025:1031	survive	1025:1031	Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001).
26650723	1	12	theme	cells	266:270	arg1	differentiation					230:244	the differentiation	226:244	the differentiation of mesenchymal stem cells into osteoblasts	226:287	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	8	13	dep	survive	1025:1031	arg1	P < 0.001					1050:1058	P < 0.001	1050:1058	P < 0.001	1050:1058	Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001).
26650723	12	14	theme	healing	1430:1436	arg1	environment					1438:1448	a favourable healing environment	1417:1448	a favourable healing environment in the root canal conducive for regenerative endodontics	1417:1505	It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.
26650723	8	15	theme	composite	985:993	arg1	scaffold					995:1002	this composite scaffold	980:1002	this composite scaffold	980:1002	Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001).
26650723	3	16	theme	stem	521:524	arg1	cells					526:530	human stem cells	515:530	human stem cells from the apical papilla (SCAPs)	515:562	Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions.
26650723	7	17	theme	gutta-percha	871:882	arg1	point					884:888	a gutta-percha point	869:888	a gutta-percha point	869:888	RESULTS The fabrication method developed enabled the shape of a gutta-percha point to be mimicked with great accuracy and reproducibility (P = 0.31).
26650723	0	18	theme	In	0:1	arg1	assessment					9:18	In vitro assessment	0:18	In vitro assessment of a	0:23	In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics.
26650723	12	19	from	environment	1438:1448	arg1	canal					1462:1466	the root canal	1453:1466	the root canal	1453:1466	It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.
26650723	2	20	theme	point-like	454:463	arg1	cone					465:468	a gutta-percha point-like cone	439:468	a gutta-percha point-like cone	439:468	METHODOLOGY A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone.
26650723	4	21	from	8 days	653:658	arg1	culture					663:669	culture	663:669	culture	663:669	The reconstructed tissue was characterized after 8 days in culture.
26650723	2	22	theme	gutta-percha	441:452	arg1	cone					465:468	a gutta-percha point-like cone	439:468	a gutta-percha point-like cone	439:468	METHODOLOGY A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone.
26650723	11	23	theme	composite	1313:1321	arg1	scaffold					1323:1330	The composite scaffold	1309:1330	The composite scaffold	1309:1330	CONCLUSIONS The composite scaffold could present substantial benefits compared to synthetic materials.
26650723	7	24	theme	fabrication	819:829	arg1	method					831:836	The fabrication method	815:836	The fabrication method developed	815:846	RESULTS The fabrication method developed enabled the shape of a gutta-percha point to be mimicked with great accuracy and reproducibility (P = 0.31).
26650723	6	25	theme	U	784:784	arg1	test					786:789	the Mann-Whitney U test	767:789	the Mann-Whitney U test	767:789	Student t-tests and the Mann-Whitney U test were performed.
26650723	2	26	dep	METHODOLOGY	339:349	arg1	scaffold					381:388	A collagen/alginate composite scaffold	351:388	METHODOLOGY A collagen/alginate composite scaffold	339:388	METHODOLOGY A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone.
26650723	1	27	theme	gutta-percha	182:193	arg1	point					195:199	a gutta-percha point	180:199	a gutta-percha point	180:199	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	12	28	theme	favourable	1419:1428	arg1	environment					1438:1448	a favourable healing environment	1417:1448	a favourable healing environment in the root canal conducive for regenerative endodontics	1417:1505	It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.
26650723	1	29	theme	point	195:199	arg1	geometry					168:175	the geometry	164:175	the geometry of a gutta-percha point with precision	164:214	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	1	29	theme	point	195:199	arg1	material					329:336	a regenerative endodontic material	303:336	a regenerative endodontic material	303:336	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	5	30	theme	cell	711:714	arg1	differentiation					716:730	cell differentiation	711:730	cell differentiation	711:730	Elastic modulus, tissue compaction and cell differentiation were assessed.
26650723	7	31	theme	great	910:914	arg1	accuracy					916:923	great accuracy	910:923	great accuracy	910:923	RESULTS The fabrication method developed enabled the shape of a gutta-percha point to be mimicked with great accuracy and reproducibility (P = 0.31).
26650723	2	32	theme	alginate	413:420	arg1	mould					422:426	a sodium alginate mould	404:426	a sodium alginate mould	404:426	METHODOLOGY A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone.
26650723	0	33	theme	a	23:23	arg1	assessment					9:18	In vitro assessment	0:18	In vitro assessment of a	0:23	In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics.
26650723	3	34	theme	cell/scaffold	576:588	arg1	interactions					590:601	cell/scaffold interactions	576:601	cell/scaffold interactions	576:601	Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions.
26650723	6	35	theme	Mann-Whitney	771:782	arg1	test					786:789	the Mann-Whitney U test	767:789	the Mann-Whitney U test	767:789	Student t-tests and the Mann-Whitney U test were performed.
26650723	2	36	theme	sodium	406:411	arg1	mould					422:426	a sodium alginate mould	404:426	a sodium alginate mould	404:426	METHODOLOGY A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone.
26650723	11	37	theme	substantial	1346:1356	arg1	benefits					1358:1365	substantial benefits	1346:1365	substantial benefits	1346:1365	CONCLUSIONS The composite scaffold could present substantial benefits compared to synthetic materials.
26650723	5	38	theme	Elastic	672:678	arg1	modulus					680:686	Elastic modulus	672:686	Elastic modulus	672:686	Elastic modulus, tissue compaction and cell differentiation were assessed.
26650723	1	39	theme	biological	109:118	arg1	scaffold					120:127	a biological scaffold	107:127	a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material	107:336	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	0	40	theme	composite	43:51	arg1	scaffold					53:60	composite scaffold	43:60	composite scaffold for regenerative endodontics	43:89	In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics.
26650723	9	41	theme	osseous	1143:1149	arg1	P < 0.001					1173:1181	P < 0.001	1173:1181	P < 0.001	1173:1181	Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001).
26650723	9	41	theme	osseous	1143:1149	arg1	matrix					1165:1170	calcified osseous extracellular matrix	1133:1170	calcified osseous extracellular matrix (P < 0.001)	1133:1182	Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001).
26650723	1	42	theme	regenerative	305:316	arg1	material					329:336	a regenerative endodontic material	303:336	a regenerative endodontic material	303:336	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	1	42	theme	regenerative	305:316	arg1	geometry					168:175	the geometry	164:175	the geometry of a gutta-percha point with precision	164:214	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	12	43	theme	regenerative	1482:1493	arg1	endodontics					1495:1505	regenerative endodontics	1482:1505	regenerative endodontics	1482:1505	It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.
26650723	12	44	theme	further	1563:1569	arg1	studies					1571:1577	further studies	1563:1577	further studies	1563:1577	It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.
26650723	9	45	theme	calcified	1133:1141	arg1	P < 0.001					1173:1181	P < 0.001	1173:1181	P < 0.001	1173:1181	Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001).
26650723	9	45	theme	calcified	1133:1141	arg1	matrix					1165:1170	calcified osseous extracellular matrix	1133:1170	calcified osseous extracellular matrix (P < 0.001)	1133:1182	Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001).
26650723	12	46	theme	conducive	1468:1476	arg1	environment					1438:1448	a favourable healing environment	1417:1448	a favourable healing environment in the root canal conducive for regenerative endodontics	1417:1505	It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.
26650723	3	47	theme	apical	541:546	arg1	papilla					548:554	the apical papilla	537:554	the apical papilla (SCAPs)	537:562	Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions.
26650723	3	47	theme	apical	541:546	arg1	SCAPs					557:561	SCAPs	557:561	SCAPs	557:561	Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions.
26650723	1	48	with	geometry	168:175	arg1	precision					206:214	precision	206:214	precision	206:214	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	10	49	theme	tip	1272:1274	arg1	P = 0.58					1286:1293	P = 0.58	1286:1293	P = 0.58	1286:1293	The construct showed no significant contraction after 8 days, preserving its shape and tip diameter (P = 0.58).
26650723	10	49	theme	tip	1272:1274	arg1	diameter					1276:1283	tip diameter	1272:1283	tip diameter (P = 0.58)	1272:1294	The construct showed no significant contraction after 8 days, preserving its shape and tip diameter (P = 0.58).
26650723	6	50	theme	Student	747:753	arg1	t-tests					755:761	Student t-tests	747:761	Student t-tests	747:761	Student t-tests and the Mann-Whitney U test were performed.
26650723	3	51	theme	human	515:519	arg1	cells					526:530	human stem cells	515:530	human stem cells from the apical papilla (SCAPs)	515:562	Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions.
26650723	0	52	theme	regenerative	66:77	arg1	endodontics					79:89	regenerative endodontics	66:89	regenerative endodontics	66:89	In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics.
26650723	9	53	theme	extracellular	1151:1163	arg1	P < 0.001					1173:1181	P < 0.001	1173:1181	P < 0.001	1173:1181	Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001).
26650723	9	53	theme	extracellular	1151:1163	arg1	matrix					1165:1170	calcified osseous extracellular matrix	1133:1170	calcified osseous extracellular matrix (P < 0.001)	1133:1182	Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001).
26650723	3	54	from	papilla	548:554	arg1	cells					526:530	human stem cells	515:530	human stem cells from the apical papilla (SCAPs)	515:562	Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions.
26650723	4	55	theme	reconstructed	608:620	arg1	tissue					622:627	The reconstructed tissue	604:627	The reconstructed tissue	604:627	The reconstructed tissue was characterized after 8 days in culture.
26650723	11	56	dep	CONCLUSIONS	1297:1307	arg1	present					1338:1344	present	1338:1344	could present substantial benefits compared to synthetic materials	1332:1397	CONCLUSIONS The composite scaffold could present substantial benefits compared to synthetic materials.
26650723	1	57	theme	endodontic	318:327	arg1	material					329:336	a regenerative endodontic material	303:336	a regenerative endodontic material	303:336	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	1	57	theme	endodontic	318:327	arg1	geometry					168:175	the geometry	164:175	the geometry of a gutta-percha point with precision	164:214	AIM To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.
26650723	0	58	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics.
26650723	2	59	theme	composite	371:379	arg1	scaffold					381:388	A collagen/alginate composite scaffold	351:388	METHODOLOGY A collagen/alginate composite scaffold	339:388	METHODOLOGY A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone.
24771686	2	0	with	demand	417:422	arg1	regards					429:435	regards	429:435	regards to proper biomechanical strength	429:468	Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength.
24771686	1	1	theme	underlying	184:193	arg1	bone					207:210	the underlying subchondral bone	180:210	the underlying subchondral bone	180:210	Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge.
24771686	6	2	theme	repairing	1220:1228	arg1	scores					1230:1235	greatly improved repairing scores	1203:1235	greatly improved repairing scores	1203:1235	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	0	3	theme	biphasic	99:106	arg1	scaffold					108:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	Osteochondral regeneration using an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold.
24771686	3	4	theme	Col-I/Hhyaluronate	680:697	arg1	phase					713:717	a freeze-dried Col-I/Hhyaluronate (HA) chondral phase	665:717	a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	665:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	4	theme	Col-I/Hhyaluronate	680:697	arg1	SPONGE					720:725	SPONGE	720:725	SPONGE	720:725	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	1	5	theme	subchondral	195:205	arg1	bone					207:210	the underlying subchondral bone	180:210	the underlying subchondral bone	180:210	Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge.
24771686	3	6	theme	liquid	617:622	arg1	electrospinning					624:638	dynamic liquid electrospinning	609:638	dynamic liquid electrospinning	609:638	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	6	7	theme	compressive	1241:1251	arg1	modulus					1253:1259	compressive modulus	1241:1259	compressive modulus	1241:1259	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	5	8	theme	biphasic	989:996	arg1	scaffold					998:1005	the Yarn-CH/TCP biphasic scaffold	973:1005	the Yarn-CH/TCP biphasic scaffold	973:1005	Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying.
24771686	2	9	theme	promising	334:342	arg1	approach					344:351	a promising approach	332:351	a promising approach for osteochondral repair	332:376	Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength.
24771686	5	10	theme	osseous	958:964	arg1	phase					966:970	the osseous phase	954:970	the osseous phase	954:970	Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying.
24771686	5	10	theme	osseous	958:964	arg1	phosphate					935:943	porous beta-tricalcium phosphate	912:943	porous beta-tricalcium phosphate (TCP)	912:949	Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying.
24771686	3	11	theme	type	560:563	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	11	theme	type	560:563	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	12	theme	ε-caprolactone	525:538	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	12	theme	ε-caprolactone	525:538	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	13	theme	/collagen	550:558	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	13	theme	/collagen	550:558	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	14	theme	-co-poly	516:523	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	14	theme	-co-poly	516:523	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	4	15	theme	Col-I/HA	816:823	arg1	Yarn-CH					842:848	Yarn-CH	842:848	Yarn-CH	842:848	In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) can allow the cell infiltration like sponge scaffolds.
24771686	4	15	theme	Col-I/HA	816:823	arg1	scaffold					832:839	the Yarn Col-I/HA hybrid scaffold	807:839	the Yarn Col-I/HA hybrid scaffold (Yarn-CH)	807:849	In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) can allow the cell infiltration like sponge scaffolds.
24771686	3	16	theme	LLA-CL	543:548	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	16	theme	LLA-CL	543:548	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	17	theme	chondral	704:711	arg1	phase					713:717	a freeze-dried Col-I/Hhyaluronate (HA) chondral phase	665:717	a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	665:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	17	theme	chondral	704:711	arg1	SPONGE					720:725	SPONGE	720:725	SPONGE	720:725	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	4	18	theme	Yarn	811:814	arg1	Yarn-CH					842:848	Yarn-CH	842:848	Yarn-CH	842:848	In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) can allow the cell infiltration like sponge scaffolds.
24771686	4	18	theme	Yarn	811:814	arg1	scaffold					832:839	the Yarn Col-I/HA hybrid scaffold	807:839	the Yarn Col-I/HA hybrid scaffold (Yarn-CH)	807:849	In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) can allow the cell infiltration like sponge scaffolds.
24771686	6	19	theme	biphasic	1105:1112	arg1	complex					1114:1120	the biphasic complex	1101:1120	the biphasic complex	1101:1120	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	4	20	dep	In	780:781	arg1	vitro					783:787	vitro	783:787	vitro	783:787	In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) can allow the cell infiltration like sponge scaffolds.
24771686	6	21	theme	improved	1211:1218	arg1	scores					1230:1235	greatly improved repairing scores	1203:1235	greatly improved repairing scores	1203:1235	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	3	22	theme	Col-I	567:571	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	22	theme	Col-I	567:571	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	0	23	theme	Osteochondral	0:12	arg1	regeneration					14:25	Osteochondral regeneration	0:25	Osteochondral regeneration	0:25	Osteochondral regeneration using an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold.
24771686	4	24	theme	hybrid	825:830	arg1	Yarn-CH					842:848	Yarn-CH	842:848	Yarn-CH	842:848	In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) can allow the cell infiltration like sponge scaffolds.
24771686	4	24	theme	hybrid	825:830	arg1	scaffold					832:839	the Yarn Col-I/HA hybrid scaffold	807:839	the Yarn Col-I/HA hybrid scaffold (Yarn-CH)	807:849	In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) can allow the cell infiltration like sponge scaffolds.
24771686	1	25	theme	Osteochondral	118:130	arg1	defects					132:138	Osteochondral defects	118:138	Osteochondral defects	118:138	Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge.
24771686	1	26	theme	poor	217:220	arg1	challenge					269:277	a daunting challenge	258:277	a daunting challenge	258:277	Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge.
24771686	1	26	theme	poor	217:220	arg1	regeneration					236:247	poor osteochondral regeneration	217:247	poor osteochondral regeneration	217:247	Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge.
24771686	6	27	from	defects	1172:1178	arg1	model					1192:1196	a rabbit model	1183:1196	a rabbit model with greatly improved repairing scores and compressive modulus	1183:1259	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	4	28	theme	sponge	888:893	arg1	scaffolds					895:903	sponge scaffolds	888:903	sponge scaffolds	888:903	In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) can allow the cell infiltration like sponge scaffolds.
24771686	3	29	theme	I	565:565	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	29	theme	I	565:565	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	6	30	used	used	1139:1142	arg2	complex					1114:1120	the biphasic complex	1101:1120	the biphasic complex	1101:1120	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	1	31	theme	osteochondral	222:234	arg1	challenge					269:277	a daunting challenge	258:277	a daunting challenge	258:277	Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge.
24771686	1	31	theme	osteochondral	222:234	arg1	regeneration					236:247	poor osteochondral regeneration	217:247	poor osteochondral regeneration	217:247	Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge.
24771686	2	32	theme	osteochondral	357:369	arg1	repair					371:376	osteochondral repair	357:376	osteochondral repair	357:376	Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength.
24771686	5	33	theme	porous	912:917	arg1	phase					966:970	the osseous phase	954:970	the osseous phase	954:970	Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying.
24771686	5	33	theme	porous	912:917	arg1	TCP					946:948	TCP	946:948	TCP	946:948	Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying.
24771686	5	33	theme	porous	912:917	arg1	phosphate					935:943	porous beta-tricalcium phosphate	912:943	porous beta-tricalcium phosphate (TCP)	912:949	Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying.
24771686	5	34	theme	freeze	1029:1034	arg1	drying					1036:1041	freeze drying	1029:1041	freeze drying	1029:1041	Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying.
24771686	3	35	theme	P	541:541	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	35	theme	P	541:541	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	0	36	theme	nanofiber	45:53	arg1	scaffold					108:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	Osteochondral regeneration using an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold.
24771686	3	37	theme	dynamic	609:615	arg1	electrospinning					624:638	dynamic liquid electrospinning	609:638	dynamic liquid electrospinning	609:638	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	6	38	theme	rabbit	1185:1190	arg1	model					1192:1196	a rabbit model	1183:1196	a rabbit model with greatly improved repairing scores and compressive modulus	1183:1259	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	6	39	theme	cells	1094:1098	arg1	combination					1050:1060	combination	1050:1060	combination of bone marrow mesenchymal stem cells	1050:1098	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	2	40	theme	biomechanical	447:459	arg1	strength					461:468	proper biomechanical strength	440:468	proper biomechanical strength	440:468	Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength.
24771686	0	41	theme	oriented	36:43	arg1	scaffold					108:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	Osteochondral regeneration using an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold.
24771686	6	42	theme	stem	1089:1092	arg1	cells					1094:1098	bone marrow mesenchymal stem cells	1065:1098	bone marrow mesenchymal stem cells	1065:1098	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	5	43	theme	Yarn-CH/TCP	977:987	arg1	scaffold					998:1005	the Yarn-CH/TCP biphasic scaffold	973:1005	the Yarn-CH/TCP biphasic scaffold	973:1005	Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying.
24771686	2	44	theme	proper	440:445	arg1	strength					461:468	proper biomechanical strength	440:468	proper biomechanical strength	440:468	Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength.
24771686	0	45	theme	type	69:72	arg1	scaffold					108:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	Osteochondral regeneration using an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold.
24771686	3	46	theme	nanofiber	574:582	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	46	theme	nanofiber	574:582	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	6	47	theme	marrow	1070:1075	arg1	cells					1094:1098	bone marrow mesenchymal stem cells	1065:1098	bone marrow mesenchymal stem cells	1065:1098	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	2	48	theme	biphasic	388:395	arg1	scaffold					397:404	an ideal biphasic scaffold	379:404	an ideal biphasic scaffold	379:404	Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength.
24771686	0	49	theme	yarn-collagen	55:67	arg1	scaffold					108:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	Osteochondral regeneration using an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold.
24771686	3	50	theme	mechanical	743:752	arg1	strength					754:761	the mechanical strength	739:761	the mechanical strength of the scaffold	739:777	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	51	theme	l-lacticacid	503:514	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	51	theme	l-lacticacid	503:514	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	52	theme	yarn	584:587	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	52	theme	yarn	584:587	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	2	53	theme	ideal	382:386	arg1	scaffold					397:404	an ideal biphasic scaffold	379:404	an ideal biphasic scaffold	379:404	Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength.
24771686	3	54	theme	freeze-dried	667:678	arg1	phase					713:717	a freeze-dried Col-I/Hhyaluronate (HA) chondral phase	665:717	a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	665:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	54	theme	freeze-dried	667:678	arg1	SPONGE					720:725	SPONGE	720:725	SPONGE	720:725	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	2	55	theme	engineering	300:310	arg1	technology					312:321	the tissue engineering technology	289:321	the tissue engineering technology	289:321	Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength.
24771686	1	56	theme	articular	156:164	arg1	cartilage					166:174	the articular cartilage	152:174	the articular cartilage	152:174	Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge.
24771686	5	57	theme	beta-tricalcium	919:933	arg1	phase					966:970	the osseous phase	954:970	the osseous phase	954:970	Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying.
24771686	5	57	theme	beta-tricalcium	919:933	arg1	TCP					946:948	TCP	946:948	TCP	946:948	Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying.
24771686	5	57	theme	beta-tricalcium	919:933	arg1	phosphate					935:943	porous beta-tricalcium phosphate	912:943	porous beta-tricalcium phosphate (TCP)	912:949	Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying.
24771686	4	58	theme	cell	865:868	arg1	infiltration					870:881	the cell infiltration	861:881	the cell infiltration	861:881	In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) can allow the cell infiltration like sponge scaffolds.
24771686	2	59	theme	tissue	293:298	arg1	technology					312:321	the tissue engineering technology	289:321	the tissue engineering technology	289:321	Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength.
24771686	6	60	theme	mesenchymal	1077:1087	arg1	cells					1094:1098	bone marrow mesenchymal stem cells	1065:1098	bone marrow mesenchymal stem cells	1065:1098	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	1	61	theme	daunting	260:267	arg1	challenge					269:277	a daunting challenge	258:277	a daunting challenge	258:277	Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge.
24771686	1	61	theme	daunting	260:267	arg1	regeneration					236:247	poor osteochondral regeneration	217:247	poor osteochondral regeneration	217:247	Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge.
24771686	0	62	theme	hybrid/TCP	88:97	arg1	scaffold					108:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	Osteochondral regeneration using an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold.
24771686	3	63	theme	oriented	489:496	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	63	theme	oriented	489:496	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	6	64	theme	osteochondral	1158:1170	arg1	defects					1172:1178	the osteochondral defects	1154:1178	the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus	1154:1259	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	3	65	theme	poly	498:501	arg1	mesh					589:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh	486:592	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	65	theme	poly	498:501	arg1	skeleton					652:659	a skeleton	650:659	a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	650:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	2	66	theme	high	412:415	arg1	demand					417:422	high demand	412:422	high demand with regards to proper biomechanical strength	412:468	Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength.
24771686	3	67	theme	scaffold	770:777	arg1	strength					754:761	the mechanical strength	739:761	the mechanical strength of the scaffold	739:777	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	0	68	theme	I/hyaluronate	74:86	arg1	scaffold					108:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold	33:115	Osteochondral regeneration using an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold.
24771686	3	69	theme	HA	700:701	arg1	phase					713:717	a freeze-dried Col-I/Hhyaluronate (HA) chondral phase	665:717	a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE)	665:726	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	3	69	theme	HA	700:701	arg1	SPONGE					720:725	SPONGE	720:725	SPONGE	720:725	In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold.
24771686	4	70	theme	In	780:781	arg1	results					789:795	In vitro results	780:795	In vitro results	780:795	In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) can allow the cell infiltration like sponge scaffolds.
24771686	6	71	theme	bone	1065:1068	arg1	marrow					1070:1075	bone marrow	1065:1075	bone marrow mesenchymal stem cells	1065:1098	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	6	72	with	model	1192:1196	arg1	scores					1230:1235	greatly improved repairing scores	1203:1235	greatly improved repairing scores	1203:1235	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
24771686	6	72	with	model	1192:1196	arg1	modulus					1253:1259	compressive modulus	1241:1259	compressive modulus	1241:1259	After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
26116384	4	0	theme	agar	673:676	arg1	nanofibers					678:687	pure agar nanofibers	668:687	pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C)	668:781	One weight percent agar-in-DES showed improved viscoelasticity and hence, spinnability, when compared to 1 wt% agar-in-water and pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C).
26116384	7	1	theme	tailorable	1089:1098	arg1	roughness					1108:1116	tailorable surface roughness	1089:1116	tailorable surface roughness	1089:1116	These fibers were mechanically resistant, showed tailorable surface roughness and diverse size distributions, with most of the diameters falling in the sub-micron range.
26116384	4	2	theme	improved	577:584	arg1	viscoelasticity					586:600	improved viscoelasticity	577:600	improved viscoelasticity	577:600	One weight percent agar-in-DES showed improved viscoelasticity and hence, spinnability, when compared to 1 wt% agar-in-water and pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C).
26116384	7	3	theme	surface	1100:1106	arg1	roughness					1108:1116	tailorable surface roughness	1089:1116	tailorable surface roughness	1089:1116	These fibers were mechanically resistant, showed tailorable surface roughness and diverse size distributions, with most of the diameters falling in the sub-micron range.
26116384	6	4	theme	Best	961:964	arg1	fibers					976:981	Best composite fibers	961:981	Best composite fibers	961:981	Best composite fibers were formed with the 50/50 and 30/70 agar/PVA solutions.
26116384	8	5	theme	new	1318:1320	arg1	materials					1355:1363	new and highly functional agar-based materials	1318:1363	new and highly functional agar-based materials	1318:1363	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	4	6	theme	transition	763:772	arg1	temperature					740:750	the temperature	736:750	the temperature of sol-gel transition (∼80 °C)	736:781	One weight percent agar-in-DES showed improved viscoelasticity and hence, spinnability, when compared to 1 wt% agar-in-water and pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C).
26116384	5	7	theme	agar	929:932	arg1	contents					934:941	high agar contents	924:941	high agar contents (50/50 agar/PVA)	924:958	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	5	7	theme	agar	929:932	arg1	agar/PVA					950:957	50/50 agar/PVA	944:957	50/50 agar/PVA	944:957	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	4	8	theme	sol-gel	755:761	arg1	transition					763:772	sol-gel transition	755:772	sol-gel transition (∼80 °C)	755:781	One weight percent agar-in-DES showed improved viscoelasticity and hence, spinnability, when compared to 1 wt% agar-in-water and pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C).
26116384	4	8	theme	sol-gel	755:761	arg1	°C					779:780	∼80 °C	775:780	∼80 °C	775:780	One weight percent agar-in-DES showed improved viscoelasticity and hence, spinnability, when compared to 1 wt% agar-in-water and pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C).
26116384	7	9	theme	diverse	1122:1128	arg1	distributions					1135:1147	diverse size distributions	1122:1147	diverse size distributions	1122:1147	These fibers were mechanically resistant, showed tailorable surface roughness and diverse size distributions, with most of the diameters falling in the sub-micron range.
26116384	2	10	theme	eutectic	271:278	arg1	DES					289:291	DES	289:291	DES	289:291	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	2	10	theme	eutectic	271:278	arg1	solvent					280:286	the deep eutectic solvent	262:286	the deep eutectic solvent (DES)	262:292	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	2	10	theme	eutectic	271:278	arg1	chloride/urea					329:341	(2-hydroxyethyl)trimethylammonium chloride/urea	295:341	(2-hydroxyethyl)trimethylammonium chloride/urea	295:341	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	2	10	theme	eutectic	271:278	arg1	medium					398:403	an alternative solvent medium	375:403	an alternative solvent medium for agar electrospinning	375:428	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	5	11	theme	composite	902:910	arg1	fibers					912:917	composite fibers	902:917	composite fibers with high agar contents (50/50 agar/PVA)	902:958	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	8	12	contain	have	1285:1288	arg1	nano					1215:1218	Both nano and micro forms	1210:1234	nano	1215:1218	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	8	12	contain	have	1285:1288	arg1	forms					1230:1234	Both nano and micro forms	1210:1234	forms	1230:1234	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	8	12	contain	have	1285:1288	arg2	potential					1290:1298	potential	1290:1298	potential	1290:1298	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	2	13	theme	trimethylammonium	311:327	arg1	solvent					280:286	the deep eutectic solvent	262:286	the deep eutectic solvent (DES)	262:292	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	2	13	theme	trimethylammonium	311:327	arg1	chloride/urea					329:341	(2-hydroxyethyl)trimethylammonium chloride/urea	295:341	(2-hydroxyethyl)trimethylammonium chloride/urea	295:341	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	8	14	theme	micro	1224:1228	arg1	forms					1230:1234	Both nano and micro forms	1210:1234	forms	1230:1234	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	2	15	theme	deep	266:269	arg1	DES					289:291	DES	289:291	DES	289:291	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	2	15	theme	deep	266:269	arg1	solvent					280:286	the deep eutectic solvent	262:286	the deep eutectic solvent (DES)	262:292	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	2	15	theme	deep	266:269	arg1	chloride/urea					329:341	(2-hydroxyethyl)trimethylammonium chloride/urea	295:341	(2-hydroxyethyl)trimethylammonium chloride/urea	295:341	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	2	15	theme	deep	266:269	arg1	medium					398:403	an alternative solvent medium	375:403	an alternative solvent medium for agar electrospinning	375:428	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	4	16	theme	%	648:648	arg1	agar-in-water					650:662	1 wt% agar-in-water	644:662	1 wt% agar-in-water	644:662	One weight percent agar-in-DES showed improved viscoelasticity and hence, spinnability, when compared to 1 wt% agar-in-water and pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C).
26116384	7	17	dep	resistant	1071:1079	arg1	showed					1082:1087	showed	1082:1087	showed	1082:1087	These fibers were mechanically resistant, showed tailorable surface roughness and diverse size distributions, with most of the diameters falling in the sub-micron range.
26116384	6	18	theme	composite	966:974	arg1	fibers					976:981	Best composite fibers	961:981	Best composite fibers	961:981	Best composite fibers were formed with the 50/50 and 30/70 agar/PVA solutions.
26116384	3	19	theme	electrospinning	515:529	arg1	set-up					531:536	a previous electrospinning set-up	504:536	a previous electrospinning set-up	504:536	The electrospun materials were collected with an ethanol bath adapted to a previous electrospinning set-up.
26116384	4	20	theme	wt	646:647	arg1	agar-in-water					650:662	1 wt% agar-in-water	644:662	1 wt% agar-in-water	644:662	One weight percent agar-in-DES showed improved viscoelasticity and hence, spinnability, when compared to 1 wt% agar-in-water and pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C).
26116384	0	21	theme	agar	10:13	arg1	electrospinnability					15:33	agar electrospinnability	10:33	agar electrospinnability	10:33	Improving agar electrospinnability with choline-based deep eutectic solvents.
26116384	4	22	theme	weight	543:548	arg1	agar-in-DES					558:568	One weight percent agar-in-DES	539:568	One weight percent agar-in-DES	539:568	One weight percent agar-in-DES showed improved viscoelasticity and hence, spinnability, when compared to 1 wt% agar-in-water and pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C).
26116384	2	23	theme	molar	359:363	arg1	ratio					365:369	1:2 molar ratio	355:369	1:2 molar ratio	355:369	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	2	24	theme	agar	409:412	arg1	electrospinning					414:428	agar electrospinning	409:428	agar electrospinning	409:428	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	5	25	theme	high	924:927	arg1	contents					934:941	high agar contents	924:941	high agar contents (50/50 agar/PVA)	924:958	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	5	25	theme	high	924:927	arg1	agar/PVA					950:957	50/50 agar/PVA	944:957	50/50 agar/PVA	944:957	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	6	26	theme	30/70	1014:1018	arg1	solutions					1029:1037	the 50/50 and 30/70 agar/PVA solutions	1000:1037	solutions	1029:1037	Best composite fibers were formed with the 50/50 and 30/70 agar/PVA solutions.
26116384	3	27	theme	previous	506:513	arg1	set-up					531:536	a previous electrospinning set-up	504:536	a previous electrospinning set-up	504:536	The electrospun materials were collected with an ethanol bath adapted to a previous electrospinning set-up.
26116384	1	28	theme	novel	115:119	arg1	fibers					132:137	novel agar-based fibers	115:137	novel agar-based fibers	115:137	Very recently our group has produced novel agar-based fibers by an electrospinning technique using water as solvent and polyvinyl alcohol (PVA) as co-blending polymer.
26116384	7	29	theme	size	1130:1133	arg1	distributions					1135:1147	diverse size distributions	1122:1147	diverse size distributions	1122:1147	These fibers were mechanically resistant, showed tailorable surface roughness and diverse size distributions, with most of the diameters falling in the sub-micron range.
26116384	2	30	theme	solvent	390:396	arg1	solvent					280:286	the deep eutectic solvent	262:286	the deep eutectic solvent (DES)	262:292	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	2	30	theme	solvent	390:396	arg1	medium					398:403	an alternative solvent medium	375:403	an alternative solvent medium for agar electrospinning	375:428	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	0	31	theme	deep	54:57	arg1	solvents					68:75	choline-based deep eutectic solvents	40:75	choline-based deep eutectic solvents	40:75	Improving agar electrospinnability with choline-based deep eutectic solvents.
26116384	4	32	theme	percent	550:556	arg1	agar-in-DES					558:568	One weight percent agar-in-DES	539:568	One weight percent agar-in-DES	539:568	One weight percent agar-in-DES showed improved viscoelasticity and hence, spinnability, when compared to 1 wt% agar-in-water and pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C).
26116384	8	33	theme	agar	1239:1242	arg1	fibers					1244:1249	agar fibers	1239:1249	agar fibers (used separately or combined)	1239:1279	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	8	34	theme	functional	1333:1342	arg1	materials					1355:1363	new and highly functional agar-based materials	1318:1363	new and highly functional agar-based materials	1318:1363	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	1	35	theme	polyvinyl	198:206	arg1	PVA					217:219	PVA	217:219	PVA	217:219	Very recently our group has produced novel agar-based fibers by an electrospinning technique using water as solvent and polyvinyl alcohol (PVA) as co-blending polymer.
26116384	1	35	theme	polyvinyl	198:206	arg1	alcohol					208:214	polyvinyl alcohol	198:214	polyvinyl alcohol (PVA)	198:220	Very recently our group has produced novel agar-based fibers by an electrospinning technique using water as solvent and polyvinyl alcohol (PVA) as co-blending polymer.
26116384	2	36	theme	alternative	378:388	arg1	solvent					280:286	the deep eutectic solvent	262:286	the deep eutectic solvent (DES)	262:292	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	2	36	theme	alternative	378:388	arg1	medium					398:403	an alternative solvent medium	375:403	an alternative solvent medium for agar electrospinning	375:428	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	0	37	theme	choline-based	40:52	arg1	solvents					68:75	choline-based deep eutectic solvents	40:75	choline-based deep eutectic solvents	40:75	Improving agar electrospinnability with choline-based deep eutectic solvents.
26116384	5	38	with	fibers	912:917	arg1	contents					934:941	high agar contents	924:941	high agar contents (50/50 agar/PVA)	924:958	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	5	38	with	fibers	912:917	arg1	agar/PVA					950:957	50/50 agar/PVA	944:957	50/50 agar/PVA	944:957	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	5	39	theme	solvent	800:806	arg1	medium					808:813	the solvent medium	796:813	the solvent medium	796:813	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	3	40	theme	electrospun	435:445	arg1	materials					447:455	The electrospun materials	431:455	The electrospun materials	431:455	The electrospun materials were collected with an ethanol bath adapted to a previous electrospinning set-up.
26116384	5	41	theme	%	855:855	arg1	solution					866:873	5 wt% starting solution	851:873	5 wt% starting solution	851:873	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	5	41	theme	%	855:855	arg1	concentration					836:848	the PVA concentration	828:848	the PVA concentration (5 wt% starting solution)	828:874	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	8	42	theme	materials	1355:1363	arg1	design					1308:1313	the design	1304:1313	the design of new and highly functional agar-based materials	1304:1363	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	1	43	theme	agar-based	121:130	arg1	fibers					132:137	novel agar-based fibers	115:137	novel agar-based fibers	115:137	Very recently our group has produced novel agar-based fibers by an electrospinning technique using water as solvent and polyvinyl alcohol (PVA) as co-blending polymer.
26116384	0	44	theme	eutectic	59:66	arg1	solvents					68:75	choline-based deep eutectic solvents	40:75	choline-based deep eutectic solvents	40:75	Improving agar electrospinnability with choline-based deep eutectic solvents.
26116384	6	45	theme	agar/PVA	1020:1027	arg1	solutions					1029:1037	the 50/50 and 30/70 agar/PVA solutions	1000:1037	solutions	1029:1037	Best composite fibers were formed with the 50/50 and 30/70 agar/PVA solutions.
26116384	8	46	theme	agar-based	1344:1353	arg1	materials					1355:1363	new and highly functional agar-based materials	1318:1363	new and highly functional agar-based materials	1318:1363	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	3	47	theme	ethanol	480:486	arg1	bath					488:491	an ethanol bath	477:491	an ethanol bath adapted to a previous electrospinning set-up	477:536	The electrospun materials were collected with an ethanol bath adapted to a previous electrospinning set-up.
26116384	1	48	theme	electrospinning	145:159	arg1	technique					161:169	an electrospinning technique	142:169	an electrospinning technique using water as solvent and polyvinyl alcohol (PVA) as co-blending polymer	142:243	Very recently our group has produced novel agar-based fibers by an electrospinning technique using water as solvent and polyvinyl alcohol (PVA) as co-blending polymer.
26116384	2	49	theme	2-hydroxyethyl	296:309	arg1	solvent					280:286	the deep eutectic solvent	262:286	the deep eutectic solvent (DES)	262:292	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	2	49	theme	2-hydroxyethyl	296:309	arg1	chloride/urea					329:341	(2-hydroxyethyl)trimethylammonium chloride/urea	295:341	(2-hydroxyethyl)trimethylammonium chloride/urea	295:341	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	5	50	theme	wt	853:854	arg1	solution					866:873	5 wt% starting solution	851:873	5 wt% starting solution	851:873	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	5	50	theme	wt	853:854	arg1	concentration					836:848	the PVA concentration	828:848	the PVA concentration (5 wt% starting solution)	828:874	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	5	51	theme	50/50	944:948	arg1	contents					934:941	high agar contents	924:941	high agar contents (50/50 agar/PVA)	924:958	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	5	51	theme	50/50	944:948	arg1	agar/PVA					950:957	50/50 agar/PVA	944:957	50/50 agar/PVA	944:957	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	5	52	theme	starting	857:864	arg1	solution					866:873	5 wt% starting solution	851:873	5 wt% starting solution	851:873	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	5	52	theme	starting	857:864	arg1	concentration					836:848	the PVA concentration	828:848	the PVA concentration (5 wt% starting solution)	828:874	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	1	53	theme	co-blending	225:235	arg1	polymer					237:243	co-blending polymer	225:243	co-blending polymer	225:243	Very recently our group has produced novel agar-based fibers by an electrospinning technique using water as solvent and polyvinyl alcohol (PVA) as co-blending polymer.
26116384	7	54	theme	sub-micron	1192:1201	arg1	range					1203:1207	the sub-micron range	1188:1207	the sub-micron range	1188:1207	These fibers were mechanically resistant, showed tailorable surface roughness and diverse size distributions, with most of the diameters falling in the sub-micron range.
26116384	5	55	theme	PVA	832:834	arg1	solution					866:873	5 wt% starting solution	851:873	5 wt% starting solution	851:873	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	5	55	theme	PVA	832:834	arg1	concentration					836:848	the PVA concentration	828:848	the PVA concentration (5 wt% starting solution)	828:874	By changing the solvent medium we decreased the PVA concentration (5 wt% starting solution) and successfully produced composite fibers with high agar contents (50/50 agar/PVA).
26116384	2	56	theme	1:2	355:357	arg1	ratio					365:369	1:2 molar ratio	355:369	1:2 molar ratio	355:369	Here, we tested the deep eutectic solvent (DES), (2-hydroxyethyl)trimethylammonium chloride/urea prepared at 1:2 molar ratio, as an alternative solvent medium for agar electrospinning.
26116384	8	57	dep	fibers	1244:1249	arg1	used					1252:1255	used	1252:1255	used separately	1252:1266	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	8	57	dep	fibers	1244:1249	arg1	combined					1271:1278	combined	1271:1278	combined	1271:1278	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	4	58	theme	pure	668:671	arg1	nanofibers					678:687	pure agar nanofibers	668:687	pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C)	668:781	One weight percent agar-in-DES showed improved viscoelasticity and hence, spinnability, when compared to 1 wt% agar-in-water and pure agar nanofibers were successfully electrospun if working above the temperature of sol-gel transition (∼80 °C).
26116384	8	59	theme	fibers	1244:1249	arg1	nano					1215:1218	Both nano and micro forms	1210:1234	nano	1215:1218	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
26116384	8	59	theme	fibers	1244:1249	arg1	forms					1230:1234	Both nano and micro forms	1210:1234	forms	1230:1234	Both nano and micro forms of agar fibers (used separately or combined) may have potential for the design of new and highly functional agar-based materials.
25116686	5	0	from	18:2ω6	975:980	arg1	lymph					996:1000	lymph	996:1000	lymph	996:1000	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	3	1	theme	lymphatic	751:759	arg1	trunk					761:765	the jejunal lymphatic trunk	739:765	the jejunal lymphatic trunk in a cross-over design	739:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	0	2	theme	acid	74:77	arg1	profile					79:85	fatty acid profile	68:85	fatty acid profile of lymph and serum in pigs	68:112	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.
25116686	2	3	theme	cholesterol	310:320	arg1	concentrations					292:305	the systemic concentrations	279:305	the systemic concentrations of cholesterol and lipid	279:330	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	1	4	contain	have	170:173	arg2	functions					183:191	various functions	175:191	various functions	175:191	Triglycerides are absorbed by the lymphatic system and have various functions in the body.
25116686	1	4	contain	have	170:173	arg1	Triglycerides					115:127	Triglycerides	115:127	Triglycerides	115:127	Triglycerides are absorbed by the lymphatic system and have various functions in the body.
25116686	0	5	theme	lymph	90:94	arg1	profile					79:85	fatty acid profile	68:85	fatty acid profile of lymph and serum in pigs	68:112	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.
25116686	0	6	from	Effect	0:5	arg1	changes					57:63	acute postprandial changes	38:63	acute postprandial changes in fatty acid profile of lymph and serum in pigs	38:112	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.
25116686	3	7	theme	yeast	571:575	arg1	-D-glucan					589:597	yeast β-(1→3)(1→6)-D-glucan	571:597	yeast β-(1→3)(1→6)-D-glucan	571:597	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	6	8	theme	acid	1268:1271	arg1	concentration					1222:1234	the concentration	1218:1234	the concentration of arachidonic and docahexanoic acid	1218:1271	While the concentration of dietary fatty acids increased postprandially in lymph, the concentration of arachidonic and docahexanoic acid tended to decrease.
25116686	3	9	theme	g	499:499	arg1	effects					476:482	the acute effects	466:482	the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design	466:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	2	10	from	effect	269:274	arg1	concentrations					292:305	the systemic concentrations	279:305	the systemic concentrations of cholesterol and lipid	279:330	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	3	11	theme	34.7±1.1	690:697	arg1	kg					699:700	initial weight 34.7±1.1 kg	675:700	initial weight 34.7±1.1 kg	675:700	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	11	theme	34.7±1.1	690:697	arg1	pigs					669:672	10 female pigs	659:672	10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design	659:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	0	12	from	changes	57:63	arg1	profile					79:85	fatty acid profile	68:85	fatty acid profile of lymph and serum in pigs	68:112	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.
25116686	3	13	from	effects	476:482	arg1	composition					625:635	composition	625:635	composition	625:635	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	13	from	effects	476:482	arg1	content					613:619	fatty acid content	602:619	fatty acid content	602:619	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	2	14	theme	β-glucans	243:251	arg1	types					234:238	some types	229:238	some types of β-glucans	229:251	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	3	15	theme	cross-over	772:781	arg1	design					783:788	a cross-over design	770:788	a cross-over design	770:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	0	16	theme	serum	100:104	arg1	profile					79:85	fatty acid profile	68:85	fatty acid profile of lymph and serum in pigs	68:112	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.
25116686	3	17	from	composition	625:635	arg1	lymph					640:644	lymph	640:644	lymph	640:644	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	17	from	composition	625:635	arg1	serum					650:654	serum	650:654	serum	650:654	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	18	theme	ingesting	487:495	arg1	g					499:499	ingesting 2 g	487:499	ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan	487:597	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	19	attach	derived	526:532	arg1	-D-glucan					589:597	yeast β-(1→3)(1→6)-D-glucan	571:597	yeast β-(1→3)(1→6)-D-glucan	571:597	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	19	attach	derived	526:532	arg2	concentrates					513:524	β-glucan concentrates	504:524	β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan	504:597	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	19	attach	derived	526:532	arg1	-D-glucan					558:566	barley β-(1→3)(1→4)-D-glucan	539:566	barley β-(1→3)(1→4)-D-glucan	539:566	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	1	20	theme	lymphatic	149:157	arg1	system					159:164	the lymphatic system	145:164	the lymphatic system	145:164	Triglycerides are absorbed by the lymphatic system and have various functions in the body.
25116686	0	21	theme	postprandial	44:55	arg1	changes					57:63	acute postprandial changes	38:63	acute postprandial changes in fatty acid profile of lymph and serum in pigs	38:112	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.
25116686	3	22	theme	fatty	602:606	arg1	content					613:619	fatty acid content	602:619	fatty acid content	602:619	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	5	23	located	observed	1049:1056	arg2	effect					1021:1026	a significant effect	1007:1026	a significant effect of β-glucan	1007:1038	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	5	23	located	observed	1049:1056	arg1	p=0.019					1117:1123	p=0.019	1117:1123	p=0.019	1117:1123	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	5	23	located	observed	1049:1056	arg1	18:0					1111:1114	18:0	1111:1114	18:0	1111:1114	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	5	23	located	observed	1049:1056	arg1	lymph					1101:1105	lymph	1101:1105	lymph	1101:1105	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	5	24	theme	β-glucan	1031:1038	arg1	effect					1021:1026	a significant effect	1007:1026	a significant effect of β-glucan	1007:1038	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	6	25	theme	acids	1177:1181	arg1	concentration					1146:1158	the concentration	1142:1158	the concentration of dietary fatty acids	1142:1181	While the concentration of dietary fatty acids increased postprandially in lymph, the concentration of arachidonic and docahexanoic acid tended to decrease.
25116686	2	26	theme	bile	417:420	arg1	acids					422:426	bile acids	417:426	bile acids	417:426	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	3	27	theme	acid	608:611	arg1	content					613:619	fatty acid content	602:619	fatty acid content	602:619	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	5	28	from	18:3ω3	986:991	arg1	lymph					996:1000	lymph	996:1000	lymph	996:1000	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	5	29	located	observed	923:930	arg1	serum					955:959	serum	955:959	serum	955:959	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	5	29	located	observed	923:930	arg2	effect					890:895	A significant effect	876:895	A significant effect of time after feeding	876:917	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	7	30	from	concentration	1326:1338	arg1	h					1369:1369	serum 1 h	1361:1369	serum 1 h after the meal	1361:1384	Furthermore, there was a drop in concentration of all fatty acid in serum 1 h after the meal.
25116686	5	31	theme	time	900:903	arg1	effect					890:895	A significant effect	876:895	A significant effect of time after feeding	876:917	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	3	32	from	catheter	727:734	arg1	trunk					761:765	the jejunal lymphatic trunk	739:765	the jejunal lymphatic trunk in a cross-over design	739:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	2	33	theme	reabsorption	401:412	arg1	absorption					374:383	the absorption	370:383	the absorption of lipid and/or reabsorption of bile acids	370:426	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	2	34	theme	positive	260:267	arg1	effect					269:274	a positive effect	258:274	a positive effect on the systemic concentrations of cholesterol and lipid	258:330	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	7	35	theme	fatty	1347:1351	arg1	acid					1353:1356	all fatty acid	1343:1356	all fatty acid	1343:1356	Furthermore, there was a drop in concentration of all fatty acid in serum 1 h after the meal.
25116686	2	36	theme	lipid	388:392	arg1	absorption					374:383	the absorption	370:383	the absorption of lipid and/or reabsorption of bile acids	370:426	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	0	37	theme	β-glucan	10:17	arg1	supplementation					19:33	β-glucan supplementation	10:33	β-glucan supplementation	10:33	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.
25116686	3	38	dep	-D-glucan	589:597	arg1	1→6					585:587	1→6	585:587	1→6	585:587	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	7	39	from	drop	1318:1321	arg1	concentration					1326:1338	concentration	1326:1338	concentration of all fatty acid in serum 1 h after the meal	1326:1384	Furthermore, there was a drop in concentration of all fatty acid in serum 1 h after the meal.
25116686	0	40	from	profile	79:85	arg1	pigs					109:112	pigs	109:112	pigs	109:112	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.
25116686	3	41	theme	concentrates	513:524	arg1	g					499:499	ingesting 2 g	487:499	ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan	487:597	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	0	42	theme	acute	38:42	arg1	changes					57:63	acute postprandial changes	38:63	acute postprandial changes in fatty acid profile of lymph and serum in pigs	38:112	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.
25116686	2	43	contain	have	253:256	arg1	types					234:238	some types	229:238	some types of β-glucans	229:251	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	2	43	contain	have	253:256	arg2	effect					269:274	a positive effect	258:274	a positive effect on the systemic concentrations of cholesterol and lipid	258:330	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	2	44	with	interference	352:363	arg1	absorption					374:383	the absorption	370:383	the absorption of lipid and/or reabsorption of bile acids	370:426	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	3	45	theme	β-	577:578	arg1	-D-glucan					589:597	yeast β-(1→3)(1→6)-D-glucan	571:597	yeast β-(1→3)(1→6)-D-glucan	571:597	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	4	46	theme	spot	846:849	arg1	sample					851:856	a spot sample	844:856	a spot sample taken 24 h after	844:873	Lymph was collected continuously for 8 h followed by a spot sample taken 24 h after.
25116686	4	47	dep	collected	801:809	arg1	followed					832:839	followed	832:839	followed by a spot sample taken 24 h after	832:873	Lymph was collected continuously for 8 h followed by a spot sample taken 24 h after.
25116686	0	48	theme	supplementation	19:33	arg1	Effect					0:5	Effect	0:5	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.	0:113	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.
25116686	7	49	theme	serum	1361:1365	arg1	h					1369:1369	serum 1 h	1361:1369	serum 1 h after the meal	1361:1384	Furthermore, there was a drop in concentration of all fatty acid in serum 1 h after the meal.
25116686	6	50	theme	dietary	1163:1169	arg1	acids					1177:1181	dietary fatty acids	1163:1181	dietary fatty acids	1163:1181	While the concentration of dietary fatty acids increased postprandially in lymph, the concentration of arachidonic and docahexanoic acid tended to decrease.
25116686	3	51	theme	female	662:667	arg1	kg					699:700	initial weight 34.7±1.1 kg	675:700	initial weight 34.7±1.1 kg	675:700	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	51	theme	female	662:667	arg1	pigs					669:672	10 female pigs	659:672	10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design	659:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	7	52	theme	acid	1353:1356	arg1	concentration					1326:1338	concentration	1326:1338	concentration of all fatty acid in serum 1 h after the meal	1326:1384	Furthermore, there was a drop in concentration of all fatty acid in serum 1 h after the meal.
25116686	3	53	theme	barley	539:544	arg1	-D-glucan					558:566	barley β-(1→3)(1→4)-D-glucan	539:566	barley β-(1→3)(1→4)-D-glucan	539:566	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	5	54	theme	fatty	940:944	arg1	acids					946:950	all fatty acids	936:950	all fatty acids	936:950	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	2	55	theme	acids	422:426	arg1	absorption					374:383	the absorption	370:383	the absorption of lipid and/or reabsorption of bile acids	370:426	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	3	56	theme	current	436:442	arg1	study					444:448	the current study	432:448	the current study	432:448	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	5	57	theme	significant	878:888	arg1	effect					890:895	A significant effect	876:895	A significant effect of time after feeding	876:917	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	3	58	theme	β-	546:547	arg1	-D-glucan					558:566	barley β-(1→3)(1→4)-D-glucan	539:566	barley β-(1→3)(1→4)-D-glucan	539:566	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	59	theme	acute	470:474	arg1	effects					476:482	the acute effects	466:482	the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design	466:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	5	60	from	18:0	969:972	arg1	lymph					996:1000	lymph	996:1000	lymph	996:1000	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	3	61	theme	permanent	717:725	arg1	catheter					727:734	a permanent catheter	715:734	a permanent catheter in the jejunal lymphatic trunk in a cross-over design	715:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	6	62	theme	fatty	1171:1175	arg1	acids					1177:1181	dietary fatty acids	1163:1181	dietary fatty acids	1163:1181	While the concentration of dietary fatty acids increased postprandially in lymph, the concentration of arachidonic and docahexanoic acid tended to decrease.
25116686	5	63	from	18:0	1111:1114	arg1	serum					1129:1133	serum	1129:1133	serum	1129:1133	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	3	64	from	trunk	761:765	arg1	design					783:788	a cross-over design	770:788	a cross-over design	770:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	65	theme	1→3	549:551	arg1	-D-glucan					558:566	barley β-(1→3)(1→4)-D-glucan	539:566	barley β-(1→3)(1→4)-D-glucan	539:566	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	2	66	theme	systemic	283:290	arg1	concentrations					292:305	the systemic concentrations	279:305	the systemic concentrations of cholesterol and lipid	279:330	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	3	67	theme	β-glucan	504:511	arg1	concentrates					513:524	β-glucan concentrates	504:524	β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan	504:597	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	68	theme	initial	675:681	arg1	kg					699:700	initial weight 34.7±1.1 kg	675:700	initial weight 34.7±1.1 kg	675:700	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	68	theme	initial	675:681	arg1	pigs					669:672	10 female pigs	659:672	10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design	659:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	6	69	theme	docahexanoic	1255:1266	arg1	acid					1268:1271	arachidonic and docahexanoic acid	1239:1271	arachidonic and docahexanoic acid	1239:1271	While the concentration of dietary fatty acids increased postprandially in lymph, the concentration of arachidonic and docahexanoic acid tended to decrease.
25116686	3	70	dep	-D-glucan	558:566	arg1	1→4					554:556	1→4	554:556	1→4	554:556	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	71	theme	weight	683:688	arg1	kg					699:700	initial weight 34.7±1.1 kg	675:700	initial weight 34.7±1.1 kg	675:700	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	71	theme	weight	683:688	arg1	pigs					669:672	10 female pigs	659:672	10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design	659:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	0	72	theme	fatty	68:72	arg1	profile					79:85	fatty acid profile	68:85	fatty acid profile of lymph and serum in pigs	68:112	Effect of β-glucan supplementation on acute postprandial changes in fatty acid profile of lymph and serum in pigs.
25116686	3	73	theme	1→3	580:582	arg1	-D-glucan					589:597	yeast β-(1→3)(1→6)-D-glucan	571:597	yeast β-(1→3)(1→6)-D-glucan	571:597	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	5	74	from	lymph	1101:1105	arg1	serum					1129:1133	serum	1129:1133	serum	1129:1133	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	1	75	theme	various	175:181	arg1	functions					183:191	various functions	175:191	various functions	175:191	Triglycerides are absorbed by the lymphatic system and have various functions in the body.
25116686	6	76	theme	arachidonic	1239:1249	arg1	acid					1268:1271	arachidonic and docahexanoic acid	1239:1271	arachidonic and docahexanoic acid	1239:1271	While the concentration of dietary fatty acids increased postprandially in lymph, the concentration of arachidonic and docahexanoic acid tended to decrease.
25116686	3	77	theme	pigs	669:672	arg1	lymph					640:644	lymph	640:644	lymph	640:644	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	77	theme	pigs	669:672	arg1	serum					650:654	serum	650:654	serum	650:654	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	5	78	theme	significant	1009:1019	arg1	effect					1021:1026	a significant effect	1007:1026	a significant effect of β-glucan	1007:1038	A significant effect of time after feeding was observed for all fatty acids in serum and for 18:0, 18:2ω6 and 18:3ω3 in lymph, but a significant effect of β-glucan was only observed for 14:0 (p=0.049) and 22:6ω3 (p=0.048) in lymph and 18:0 (p=0.019) in serum.
25116686	2	79	theme	lipid	326:330	arg1	concentrations					292:305	the systemic concentrations	279:305	the systemic concentrations of cholesterol and lipid	279:330	It has been shown that some types of β-glucans have a positive effect on the systemic concentrations of cholesterol and lipid, presumably through interference with the absorption of lipid and/or reabsorption of bile acids.
25116686	3	80	theme	jejunal	743:749	arg1	trunk					761:765	the jejunal lymphatic trunk	739:765	the jejunal lymphatic trunk in a cross-over design	739:788	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	81	from	content	613:619	arg1	lymph					640:644	lymph	640:644	lymph	640:644	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25116686	3	81	from	content	613:619	arg1	serum					650:654	serum	650:654	serum	650:654	In the current study we investigated the acute effects of ingesting 2 g of β-glucan concentrates derived from barley β-(1→3)(1→4)-D-glucan or yeast β-(1→3)(1→6)-D-glucan on fatty acid content and composition in lymph and serum of 10 female pigs (initial weight 34.7±1.1 kg) fitted with a permanent catheter in the jejunal lymphatic trunk in a cross-over design.
25598242	8	0	theme	metagenomic	1373:1383	arg1	analysis					1385:1392	metagenomic analysis	1373:1392	metagenomic analysis	1373:1392	The results indicated that metagenomic analysis was helpful to reveal the FOS effects on the global composition of gut communities and new target for future studies.
25598242	6	1	theme	FOS-high	879:886	arg1	group					888:892	the FOS-high group	875:892	the FOS-high group	875:892	In the FOS-high group, Enterococcus was promoted and levels of Bifidobacterium and Olsenella both notably increased after FOS diet (25%) and the microbiota tended to revert to initial structure 2 weeks after FOS treatment ceased.
25598242	4	2	from	effects	627:633	arg1	bacteria					649:656	gut bacteria	645:656	gut bacteria in mice	645:664	In this study the effects of FOS on gut bacteria in mice were investigated through a 16S rRNA metagenomic analysis.
25598242	8	3	theme	communities	1465:1475	arg1	composition					1446:1456	the global composition	1435:1456	the global composition of gut communities	1435:1475	The results indicated that metagenomic analysis was helpful to reveal the FOS effects on the global composition of gut communities and new target for future studies.
25598242	8	3	theme	communities	1465:1475	arg1	target					1485:1490	new target	1481:1490	new target for future studies	1481:1509	The results indicated that metagenomic analysis was helpful to reveal the FOS effects on the global composition of gut communities and new target for future studies.
25598242	7	4	with	comparable	1175:1184	arg1	Bifidobacterium					1191:1205	Bifidobacterium	1191:1205	Bifidobacterium	1191:1205	The most striking observation was that Olsenella became a dominant genus comparable with Bifidobacterium after FOS treatment, and one strain of Olsenella, isolated from mice feces, was confirmed, for the first time, to be capable of using FOS.
25598242	3	5	theme	Previous	451:458	arg1	studies					460:466	Previous studies	451:466	Previous studies	451:466	Previous studies have mainly investigated only a few bacteria groups, and few reports analyzed the global effects of FOS on intestinal microbial communities.
25598242	1	6	theme	bifidobacteria	230:243	arg1	growth					220:225	the growth	216:225	the growth of bifidobacteria and lactobacilli	216:260	Fructo-oligosaccharides (FOS) are usually regarded as a type of prebiotic, favorably stimulating the growth of bifidobacteria and lactobacilli.
25598242	6	7	theme	FOS	1080:1082	arg1	treatment					1084:1092	FOS treatment ceased	1080:1099	FOS treatment ceased	1080:1099	In the FOS-high group, Enterococcus was promoted and levels of Bifidobacterium and Olsenella both notably increased after FOS diet (25%) and the microbiota tended to revert to initial structure 2 weeks after FOS treatment ceased.
25598242	8	8	theme	new	1481:1483	arg1	target					1485:1490	new target	1481:1490	new target for future studies	1481:1509	The results indicated that metagenomic analysis was helpful to reveal the FOS effects on the global composition of gut communities and new target for future studies.
25598242	6	9	dep	promoted	912:919	arg1	both					965:968	both	965:968	both	965:968	In the FOS-high group, Enterococcus was promoted and levels of Bifidobacterium and Olsenella both notably increased after FOS diet (25%) and the microbiota tended to revert to initial structure 2 weeks after FOS treatment ceased.
25598242	7	10	theme	striking	1111:1118	arg1	observation					1120:1130	The most striking observation	1102:1130	The most striking observation	1102:1130	The most striking observation was that Olsenella became a dominant genus comparable with Bifidobacterium after FOS treatment, and one strain of Olsenella, isolated from mice feces, was confirmed, for the first time, to be capable of using FOS.
25598242	1	11	theme	lactobacilli	249:260	arg1	growth					220:225	the growth	216:225	the growth of bifidobacteria and lactobacilli	216:260	Fructo-oligosaccharides (FOS) are usually regarded as a type of prebiotic, favorably stimulating the growth of bifidobacteria and lactobacilli.
25598242	0	12	theme	microbiota	99:108	arg1	composition					78:88	the composition	74:88	the composition of fecal microbiota in mice	74:116	Metagenomic insights into the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice.
25598242	5	13	theme	FOS	845:847	arg1	diet					849:852	FOS diet	845:852	FOS diet (5%) for 3 weeks	845:869	In the FOS-low group, the abundance of Actinobacteria significantly increased and that of Bacteroidetes decreased after FOS diet (5%) for 3 weeks.
25598242	5	13	theme	FOS	845:847	arg1	%					856:856	5%	855:856	5%	855:856	In the FOS-low group, the abundance of Actinobacteria significantly increased and that of Bacteroidetes decreased after FOS diet (5%) for 3 weeks.
25598242	4	14	theme	rRNA	698:701	arg1	analysis					715:722	a 16S rRNA metagenomic analysis	692:722	a 16S rRNA metagenomic analysis	692:722	In this study the effects of FOS on gut bacteria in mice were investigated through a 16S rRNA metagenomic analysis.
25598242	0	15	theme	fecal	93:97	arg1	microbiota					99:108	fecal microbiota	93:108	fecal microbiota in mice	93:116	Metagenomic insights into the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice.
25598242	7	16	theme	first	1306:1310	arg1	time					1312:1315	the first time	1302:1315	the first time	1302:1315	The most striking observation was that Olsenella became a dominant genus comparable with Bifidobacterium after FOS treatment, and one strain of Olsenella, isolated from mice feces, was confirmed, for the first time, to be capable of using FOS.
25598242	8	17	from	effects	1424:1430	arg1	composition					1446:1456	the global composition	1435:1456	the global composition of gut communities	1435:1475	The results indicated that metagenomic analysis was helpful to reveal the FOS effects on the global composition of gut communities and new target for future studies.
25598242	8	17	from	effects	1424:1430	arg1	target					1485:1490	new target	1481:1490	new target for future studies	1481:1509	The results indicated that metagenomic analysis was helpful to reveal the FOS effects on the global composition of gut communities and new target for future studies.
25598242	4	18	theme	16S	694:696	arg1	analysis					715:722	a 16S rRNA metagenomic analysis	692:722	a 16S rRNA metagenomic analysis	692:722	In this study the effects of FOS on gut bacteria in mice were investigated through a 16S rRNA metagenomic analysis.
25598242	3	19	from	effects	557:563	arg1	communities					596:606	intestinal microbial communities	575:606	intestinal microbial communities	575:606	Previous studies have mainly investigated only a few bacteria groups, and few reports analyzed the global effects of FOS on intestinal microbial communities.
25598242	0	20	from	effects	30:36	arg1	composition					78:88	the composition	74:88	the composition of fecal microbiota in mice	74:116	Metagenomic insights into the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice.
25598242	2	21	theme	specific	289:296	arg1	substrates					298:307	the specific substrates	285:307	the specific substrates for these target species	285:332	However, they are not the specific substrates for these target species, and other bacteria, such as Streptococcus, Escherichia, and Clostridium, have been shown to be able to utilize FOS.
25598242	2	21	theme	specific	289:296	arg1	they					272:275	they	272:275	they	272:275	However, they are not the specific substrates for these target species, and other bacteria, such as Streptococcus, Escherichia, and Clostridium, have been shown to be able to utilize FOS.
25598242	6	22	theme	FOS	994:996	arg1	diet					998:1001	FOS diet	994:1001	FOS diet (25%)	994:1007	In the FOS-high group, Enterococcus was promoted and levels of Bifidobacterium and Olsenella both notably increased after FOS diet (25%) and the microbiota tended to revert to initial structure 2 weeks after FOS treatment ceased.
25598242	6	22	theme	FOS	994:996	arg1	%					1006:1006	25%	1004:1006	25%	1004:1006	In the FOS-high group, Enterococcus was promoted and levels of Bifidobacterium and Olsenella both notably increased after FOS diet (25%) and the microbiota tended to revert to initial structure 2 weeks after FOS treatment ceased.
25598242	0	23	theme	Metagenomic	0:10	arg1	insights					12:19	Metagenomic insights	0:19	Metagenomic insights into the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice.	0:117	Metagenomic insights into the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice.
25598242	7	24	theme	FOS	1213:1215	arg1	treatment					1217:1225	FOS treatment	1213:1225	FOS treatment	1213:1225	The most striking observation was that Olsenella became a dominant genus comparable with Bifidobacterium after FOS treatment, and one strain of Olsenella, isolated from mice feces, was confirmed, for the first time, to be capable of using FOS.
25598242	5	25	theme	FOS-low	732:738	arg1	group					740:744	the FOS-low group	728:744	the FOS-low group	728:744	In the FOS-low group, the abundance of Actinobacteria significantly increased and that of Bacteroidetes decreased after FOS diet (5%) for 3 weeks.
25598242	6	26	theme	Bifidobacterium	935:949	arg1	Olsenella					955:963	Olsenella	955:963	Olsenella	955:963	In the FOS-high group, Enterococcus was promoted and levels of Bifidobacterium and Olsenella both notably increased after FOS diet (25%) and the microbiota tended to revert to initial structure 2 weeks after FOS treatment ceased.
25598242	6	26	theme	Bifidobacterium	935:949	arg1	levels					925:930	levels	925:930	levels of Bifidobacterium	925:949	In the FOS-high group, Enterococcus was promoted and levels of Bifidobacterium and Olsenella both notably increased after FOS diet (25%) and the microbiota tended to revert to initial structure 2 weeks after FOS treatment ceased.
25598242	6	27	theme	initial	1048:1054	arg1	structure					1056:1064	initial structure	1048:1064	initial structure	1048:1064	In the FOS-high group, Enterococcus was promoted and levels of Bifidobacterium and Olsenella both notably increased after FOS diet (25%) and the microbiota tended to revert to initial structure 2 weeks after FOS treatment ceased.
25598242	3	28	theme	intestinal	575:584	arg1	communities					596:606	intestinal microbial communities	575:606	intestinal microbial communities	575:606	Previous studies have mainly investigated only a few bacteria groups, and few reports analyzed the global effects of FOS on intestinal microbial communities.
25598242	3	29	theme	bacteria	504:511	arg1	groups					513:518	only a few bacteria groups	493:518	only a few bacteria groups	493:518	Previous studies have mainly investigated only a few bacteria groups, and few reports analyzed the global effects of FOS on intestinal microbial communities.
25598242	7	30	theme	comparable	1175:1184	arg1	genus					1169:1173	a dominant genus	1158:1173	a dominant genus comparable with Bifidobacterium	1158:1205	The most striking observation was that Olsenella became a dominant genus comparable with Bifidobacterium after FOS treatment, and one strain of Olsenella, isolated from mice feces, was confirmed, for the first time, to be capable of using FOS.
25598242	6	31	dep	weeks	1068:1072	arg1	treatment					1084:1092	FOS treatment ceased	1080:1099	FOS treatment ceased	1080:1099	In the FOS-high group, Enterococcus was promoted and levels of Bifidobacterium and Olsenella both notably increased after FOS diet (25%) and the microbiota tended to revert to initial structure 2 weeks after FOS treatment ceased.
25598242	3	32	theme	microbial	586:594	arg1	communities					596:606	intestinal microbial communities	575:606	intestinal microbial communities	575:606	Previous studies have mainly investigated only a few bacteria groups, and few reports analyzed the global effects of FOS on intestinal microbial communities.
25598242	1	33	theme	prebiotic	183:191	arg1	Fructo-oligosaccharides					119:141	Fructo-oligosaccharides	119:141	Fructo-oligosaccharides (FOS)	119:147	Fructo-oligosaccharides (FOS) are usually regarded as a type of prebiotic, favorably stimulating the growth of bifidobacteria and lactobacilli.
25598242	1	33	theme	prebiotic	183:191	arg1	type					175:178	a type	173:178	a type of prebiotic	173:191	Fructo-oligosaccharides (FOS) are usually regarded as a type of prebiotic, favorably stimulating the growth of bifidobacteria and lactobacilli.
25598242	8	34	theme	FOS	1420:1422	arg1	effects					1424:1430	the FOS effects	1416:1430	the FOS effects on the global composition of gut communities and new target for future studies	1416:1509	The results indicated that metagenomic analysis was helpful to reveal the FOS effects on the global composition of gut communities and new target for future studies.
25598242	7	35	attach	isolated	1257:1264	arg1	feces					1276:1280	mice feces	1271:1280	mice feces	1271:1280	The most striking observation was that Olsenella became a dominant genus comparable with Bifidobacterium after FOS treatment, and one strain of Olsenella, isolated from mice feces, was confirmed, for the first time, to be capable of using FOS.
25598242	7	35	attach	isolated	1257:1264	arg2	strain					1236:1241	one strain	1232:1241	one strain	1232:1241	The most striking observation was that Olsenella became a dominant genus comparable with Bifidobacterium after FOS treatment, and one strain of Olsenella, isolated from mice feces, was confirmed, for the first time, to be capable of using FOS.
25598242	3	36	theme	FOS	568:570	arg1	effects					557:563	the global effects	546:563	the global effects of FOS on intestinal microbial communities	546:606	Previous studies have mainly investigated only a few bacteria groups, and few reports analyzed the global effects of FOS on intestinal microbial communities.
25598242	5	37	theme	Actinobacteria	764:777	arg1	abundance					751:759	the abundance	747:759	the abundance of Actinobacteria	747:777	In the FOS-low group, the abundance of Actinobacteria significantly increased and that of Bacteroidetes decreased after FOS diet (5%) for 3 weeks.
25598242	7	38	theme	Olsenella	1246:1254	arg1	strain					1236:1241	one strain	1232:1241	one strain	1232:1241	The most striking observation was that Olsenella became a dominant genus comparable with Bifidobacterium after FOS treatment, and one strain of Olsenella, isolated from mice feces, was confirmed, for the first time, to be capable of using FOS.
25598242	2	39	theme	target	319:324	arg1	species					326:332	these target species	313:332	these target species	313:332	However, they are not the specific substrates for these target species, and other bacteria, such as Streptococcus, Escherichia, and Clostridium, have been shown to be able to utilize FOS.
25598242	0	40	theme	fructo-oligosaccharides	41:63	arg1	effects					30:36	the effects	26:36	the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice	26:116	Metagenomic insights into the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice.
25598242	0	41	from	microbiota	99:108	arg1	mice					113:116	mice	113:116	mice	113:116	Metagenomic insights into the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice.
25598242	3	42	theme	few	525:527	arg1	reports					529:535	few reports	525:535	few reports	525:535	Previous studies have mainly investigated only a few bacteria groups, and few reports analyzed the global effects of FOS on intestinal microbial communities.
25598242	4	43	theme	FOS	638:640	arg1	effects					627:633	the effects	623:633	the effects of FOS on gut bacteria in mice	623:664	In this study the effects of FOS on gut bacteria in mice were investigated through a 16S rRNA metagenomic analysis.
25598242	4	44	from	bacteria	649:656	arg1	mice					661:664	mice	661:664	mice	661:664	In this study the effects of FOS on gut bacteria in mice were investigated through a 16S rRNA metagenomic analysis.
25598242	8	45	theme	gut	1461:1463	arg1	communities					1465:1475	gut communities	1461:1475	gut communities	1461:1475	The results indicated that metagenomic analysis was helpful to reveal the FOS effects on the global composition of gut communities and new target for future studies.
25598242	7	46	theme	mice	1271:1274	arg1	feces					1276:1280	mice feces	1271:1280	mice feces	1271:1280	The most striking observation was that Olsenella became a dominant genus comparable with Bifidobacterium after FOS treatment, and one strain of Olsenella, isolated from mice feces, was confirmed, for the first time, to be capable of using FOS.
25598242	8	47	theme	global	1439:1444	arg1	composition					1446:1456	the global composition	1435:1456	the global composition of gut communities	1435:1475	The results indicated that metagenomic analysis was helpful to reveal the FOS effects on the global composition of gut communities and new target for future studies.
25598242	7	48	theme	dominant	1160:1167	arg1	genus					1169:1173	a dominant genus	1158:1173	a dominant genus comparable with Bifidobacterium	1158:1205	The most striking observation was that Olsenella became a dominant genus comparable with Bifidobacterium after FOS treatment, and one strain of Olsenella, isolated from mice feces, was confirmed, for the first time, to be capable of using FOS.
25598242	4	49	theme	metagenomic	703:713	arg1	analysis					715:722	a 16S rRNA metagenomic analysis	692:722	a 16S rRNA metagenomic analysis	692:722	In this study the effects of FOS on gut bacteria in mice were investigated through a 16S rRNA metagenomic analysis.
25598242	4	50	theme	gut	645:647	arg1	bacteria					649:656	gut bacteria	645:656	gut bacteria in mice	645:664	In this study the effects of FOS on gut bacteria in mice were investigated through a 16S rRNA metagenomic analysis.
25598242	0	51	from	composition	78:88	arg1	mice					113:116	mice	113:116	mice	113:116	Metagenomic insights into the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice.
25598242	2	52	theme	other	339:343	arg1	bacteria					345:352	other bacteria	339:352	other bacteria	339:352	However, they are not the specific substrates for these target species, and other bacteria, such as Streptococcus, Escherichia, and Clostridium, have been shown to be able to utilize FOS.
25598242	2	52	theme	other	339:343	arg1	Escherichia					378:388	Escherichia	378:388	Escherichia	378:388	However, they are not the specific substrates for these target species, and other bacteria, such as Streptococcus, Escherichia, and Clostridium, have been shown to be able to utilize FOS.
25598242	2	52	theme	other	339:343	arg1	Clostridium					395:405	Clostridium	395:405	Clostridium	395:405	However, they are not the specific substrates for these target species, and other bacteria, such as Streptococcus, Escherichia, and Clostridium, have been shown to be able to utilize FOS.
25598242	2	52	theme	other	339:343	arg1	Streptococcus					363:375	Streptococcus	363:375	Streptococcus	363:375	However, they are not the specific substrates for these target species, and other bacteria, such as Streptococcus, Escherichia, and Clostridium, have been shown to be able to utilize FOS.
25598242	8	53	theme	future	1496:1501	arg1	studies					1503:1509	future studies	1496:1509	future studies	1496:1509	The results indicated that metagenomic analysis was helpful to reveal the FOS effects on the global composition of gut communities and new target for future studies.
25598242	0	54	from	mice	113:116	arg1	composition					78:88	the composition	74:88	the composition of fecal microbiota in mice	74:116	Metagenomic insights into the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice.
25598242	3	55	theme	global	550:555	arg1	effects					557:563	the global effects	546:563	the global effects of FOS on intestinal microbial communities	546:606	Previous studies have mainly investigated only a few bacteria groups, and few reports analyzed the global effects of FOS on intestinal microbial communities.
28574385	3	0	theme	leading	601:607	arg1	syndrome					622:629	leading arthropathic syndrome	601:629	leading arthropathic syndrome	601:629	The main group included 40 women with MS with leading arthropathic syndrome.
28574385	4	1	from	composition	876:886	arg1	serum					917:921	the serum	913:921	the serum	913:921	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	8	2	theme	increased	1842:1850	arg1	content					1852:1858	an increased content	1839:1858	an increased content of chondroitin sulfates	1839:1882	The arthropathic syndrome availability also confirms the significant reduction CI-6s / CI-4s ratio and an increased content of chondroitin sulfates.
28574385	3	3	with	women	582:586	arg1	syndrome					622:629	leading arthropathic syndrome	601:629	leading arthropathic syndrome	601:629	The main group included 40 women with MS with leading arthropathic syndrome.
28574385	3	3	with	women	582:586	arg1	MS					593:594	MS	593:594	MS	593:594	The main group included 40 women with MS with leading arthropathic syndrome.
28574385	6	4	theme	severe	1464:1469	arg1	changes					1482:1488	more severe dystrophic changes	1459:1488	more severe dystrophic changes	1459:1488	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	1	5	theme	connective	157:166	arg1	tissue					168:173	the connective tissue metabolic features among women with menopausal syndrome (MS) and MS	153:241	tissue	168:173	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	2	6	theme	surgery	546:552	arg1	result					534:539	a result	532:539	a result of a surgery	532:552	We examined 70 women in total, menopause duration they accounted for 1,95±1,1 years, it came both as natural, and as a result of a surgery.
28574385	1	7	with	women	200:204	arg1	MS					232:233	MS	232:233	MS	232:233	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	1	7	with	women	200:204	arg1	syndrome					222:229	menopausal syndrome	211:229	menopausal syndrome (MS)	211:234	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	9	8	theme	menopause	2111:2119	arg1	course					2093:2098	a longer and more invasive pathologic course	2055:2098	a longer and more invasive pathologic course of natural menopause	2055:2119	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	8	9	theme	sulfates	1875:1882	arg1	content					1852:1858	an increased content	1839:1858	an increased content of chondroitin sulfates	1839:1882	The arthropathic syndrome availability also confirms the significant reduction CI-6s / CI-4s ratio and an increased content of chondroitin sulfates.
28574385	8	9	theme	sulfates	1875:1882	arg1	ratio					1829:1833	the significant reduction CI-6s / CI-4s ratio	1789:1833	the significant reduction CI-6s / CI-4s ratio	1789:1833	The arthropathic syndrome availability also confirms the significant reduction CI-6s / CI-4s ratio and an increased content of chondroitin sulfates.
28574385	1	10	theme	metabolic	175:183	arg1	features					185:192	metabolic features	175:192	metabolic features among women with menopausal syndrome (MS)	175:234	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	7	11	theme	cartilage	1725:1733	arg1	destruction					1710:1720	pre-emptive destruction	1698:1720	pre-emptive destruction of cartilage	1698:1733	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group indicates the presence of pre-emptive destruction of cartilage.
28574385	4	12	from	content	852:858	arg1	serum					917:921	the serum	913:921	the serum	913:921	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	7	13	theme	glycosaminoglycans	1577:1594	arg1	composition					1562:1572	the fractional composition	1547:1572	the fractional composition of glycosaminoglycans	1547:1594	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group indicates the presence of pre-emptive destruction of cartilage.
28574385	7	14	from	increase	1608:1615	arg1	patients					1645:1652	patients	1645:1652	patients of the main group	1645:1670	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group indicates the presence of pre-emptive destruction of cartilage.
28574385	6	15	theme	glycoprotein	1253:1264	arg1	content					1219:1225	an increased content	1206:1225	an increased content of glycosaminoglycans and glycoprotein	1206:1264	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	7	16	theme	composition	1562:1572	arg1	Redistribution					1529:1542	Redistribution	1529:1542	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group	1529:1670	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group indicates the presence of pre-emptive destruction of cartilage.
28574385	4	17	theme	index	819:823	arg1	calculation					780:790	the calculation	776:790	the calculation of the clinical-functional index of WOMAC	776:832	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	9	18	theme	pronounced	1939:1948	arg1	changes					1950:1956	more pronounced changes	1934:1956	more pronounced changes	1934:1956	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	6	19	theme	glycosaminoglycans	1230:1247	arg1	content					1219:1225	an increased content	1206:1225	an increased content of glycosaminoglycans and glycoprotein	1206:1264	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	6	20	theme	symptoms	1519:1526	arg1	severity					1498:1505	the severity	1494:1505	the severity of clinical symptoms	1494:1526	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	6	20	theme	symptoms	1519:1526	arg1	changes					1482:1488	more severe dystrophic changes	1459:1488	more severe dystrophic changes	1459:1488	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	7	21	theme	pre-emptive	1698:1708	arg1	destruction					1710:1720	pre-emptive destruction	1698:1720	pre-emptive destruction of cartilage	1698:1733	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group indicates the presence of pre-emptive destruction of cartilage.
28574385	4	22	theme	WOMAC	828:832	arg1	index					819:823	the clinical-functional index	795:823	the clinical-functional index of WOMAC	795:832	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	5	23	dep	components	1147:1156	arg1	method					1158:1163	method	1158:1163	main components method	1142:1163	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	5	24	theme	main	1142:1145	arg1	components					1147:1156	main components	1142:1156	main components method	1142:1163	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	4	25	theme	modified	646:653	arg1	index					666:670	The patients' modified menopausal index	632:670	The patients' modified menopausal index	632:670	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	8	26	theme	syndrome	1753:1760	arg1	availability					1762:1773	The arthropathic syndrome availability	1736:1773	The arthropathic syndrome availability	1736:1773	The arthropathic syndrome availability also confirms the significant reduction CI-6s / CI-4s ratio and an increased content of chondroitin sulfates.
28574385	9	27	theme	tissue	1972:1977	arg1	metabolites					1979:1989	connective tissue metabolites	1961:1989	connective tissue metabolites	1961:1989	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	6	28	theme	age-related	1313:1323	arg1	rearrangement					1325:1337	the general age-related rearrangement	1301:1337	the general age-related rearrangement of connective tissue metabolism	1301:1369	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	5	29	theme	control	1044:1050	arg1	indicators					1063:1072	the control (standard) indicators	1040:1072	the control (standard) indicators	1040:1072	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	2	30	theme	menopause	446:454	arg1	duration					456:463	menopause duration	446:463	menopause duration	446:463	We examined 70 women in total, menopause duration they accounted for 1,95±1,1 years, it came both as natural, and as a result of a surgery.
28574385	0	31	theme	VARIOUS	78:84	arg1	TYPES					86:90	VARIOUS TYPES	78:90	VARIOUS TYPES OF MENOPAUSE	78:103	[CONNECTIVE TISSUE METABOLIC FEATURES IN CLIMACTERIC SYNDROME'S PATIENTS WITH VARIOUS TYPES OF MENOPAUSE].
28574385	5	32	theme	standard	1053:1060	arg1	indicators					1063:1072	the control (standard) indicators	1040:1072	the control (standard) indicators	1040:1072	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	4	33	from	acids	975:979	arg1	serum					917:921	the serum	913:921	the serum	913:921	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	7	34	theme	main	1661:1664	arg1	group					1666:1670	the main group	1657:1670	the main group	1657:1670	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group indicates the presence of pre-emptive destruction of cartilage.
28574385	4	35	theme	qualitative	864:874	arg1	composition					876:886	qualitative composition	864:886	qualitative composition	864:886	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	5	36	from	degree	1015:1020	arg1	indicators					1063:1072	the control (standard) indicators	1040:1072	the control (standard) indicators	1040:1072	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	1	37	theme	connective	322:331	arg1	indicators					340:349	the connective tissue indicators	318:349	the connective tissue indicators	318:349	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	8	38	theme	reduction	1805:1813	arg1	ratio					1829:1833	the significant reduction CI-6s / CI-4s ratio	1789:1833	the significant reduction CI-6s / CI-4s ratio	1789:1833	The arthropathic syndrome availability also confirms the significant reduction CI-6s / CI-4s ratio and an increased content of chondroitin sulfates.
28574385	1	39	theme	indicators	340:349	arg1	analysis					306:313	comparative analysis	294:313	comparative analysis of the connective tissue indicators	294:349	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	0	40	theme	CLIMACTERIC	41:51	arg1	PATIENTS					64:71	CLIMACTERIC SYNDROME'S PATIENTS	41:71	CLIMACTERIC SYNDROME'S PATIENTS WITH VARIOUS TYPES OF MENOPAUSE	41:103	[CONNECTIVE TISSUE METABOLIC FEATURES IN CLIMACTERIC SYNDROME'S PATIENTS WITH VARIOUS TYPES OF MENOPAUSE].
28574385	4	41	theme	hydroxyproline	949:962	arg1	composition					876:886	qualitative composition	864:886	qualitative composition	864:886	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	4	41	theme	hydroxyproline	949:962	arg1	acids					975:979	uronic acids	968:979	uronic acids	968:979	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	4	41	theme	hydroxyproline	949:962	arg1	excretion					936:944	the urinary excretion	924:944	the urinary excretion of hydroxyproline	924:962	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	4	41	theme	hydroxyproline	949:962	arg1	content					852:858	quantitative content	839:858	quantitative content	839:858	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	5	42	theme	factor	1125:1130	arg1	analysis					1132:1139	factor analysis	1125:1139	factor analysis	1125:1139	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	9	43	theme	surgical	2021:2028	arg1	menopause					2030:2038	surgical menopause	2021:2038	surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause	2021:2119	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	5	44	theme	Mann-Whitney	1098:1109	arg1	criterion					1111:1119	non-parametric Mann-Whitney criterion	1083:1119	non-parametric Mann-Whitney criterion	1083:1119	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	7	45	theme	chondroitin-6	1620:1632	arg1	sulfate					1634:1640	chondroitin-6 sulfate	1620:1640	chondroitin-6 sulfate	1620:1640	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group indicates the presence of pre-emptive destruction of cartilage.
28574385	4	46	theme	uronic	968:973	arg1	acids					975:979	uronic acids	968:979	uronic acids	968:979	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	1	47	dep	tissue	168:173	arg1	features					185:192	metabolic features	175:192	metabolic features among women with menopausal syndrome (MS)	175:234	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	0	48	theme	METABOLIC	19:27	arg1	FEATURES					29:36	TISSUE METABOLIC FEATURES	12:36	TISSUE METABOLIC FEATURES IN CLIMACTERIC SYNDROME'S PATIENTS WITH VARIOUS TYPES OF MENOPAUSE	12:103	[CONNECTIVE TISSUE METABOLIC FEATURES IN CLIMACTERIC SYNDROME'S PATIENTS WITH VARIOUS TYPES OF MENOPAUSE].
28574385	1	49	dep	analysis	306:313	arg1	exchange					351:358	exchange	351:358	exchange	351:358	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	4	50	theme	joints	726:731	arg1	condition					709:717	the functional condition	694:717	the functional condition of the joints	694:731	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	6	51	theme	tissue	1353:1358	arg1	metabolism					1360:1369	connective tissue metabolism	1342:1369	connective tissue metabolism	1342:1369	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	4	52	from	excretion	936:944	arg1	serum					917:921	the serum	913:921	the serum	913:921	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	1	53	theme	comparative	294:304	arg1	analysis					306:313	comparative analysis	294:313	comparative analysis of the connective tissue indicators	294:349	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	9	54	theme	longer	2057:2062	arg1	course					2093:2098	a longer and more invasive pathologic course	2055:2098	a longer and more invasive pathologic course of natural menopause	2055:2119	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	9	55	theme	invasive	2073:2080	arg1	course					2093:2098	a longer and more invasive pathologic course	2055:2098	a longer and more invasive pathologic course of natural menopause	2055:2119	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	4	56	theme	functional	698:707	arg1	condition					709:717	the functional condition	694:717	the functional condition of the joints	694:731	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	6	57	theme	expressed	1417:1425	arg1	indictors					1427:1435	more expressed indictors	1412:1435	more expressed indictors	1412:1435	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	1	58	theme	menopausal	211:220	arg1	MS					232:233	MS	232:233	MS	232:233	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	1	58	theme	menopausal	211:220	arg1	syndrome					222:229	menopausal syndrome	211:229	menopausal syndrome (MS)	211:234	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	9	59	theme	natural	2103:2109	arg1	menopause					2111:2119	natural menopause	2103:2119	natural menopause	2103:2119	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	6	60	theme	increased	1209:1217	arg1	content					1219:1225	an increased content	1206:1225	an increased content of glycosaminoglycans and glycoprotein	1206:1264	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	6	61	theme	main	1396:1399	arg1	group					1401:1405	the main group	1392:1405	the main group	1392:1405	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	3	62	theme	arthropathic	609:620	arg1	syndrome					622:629	leading arthropathic syndrome	601:629	leading arthropathic syndrome	601:629	The main group included 40 women with MS with leading arthropathic syndrome.
28574385	5	63	from	indicators	1063:1072	arg1	deviation					1025:1033	deviation	1025:1033	deviation from the control (standard) indicators	1025:1072	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	5	63	from	indicators	1063:1072	arg1	degree					1015:1020	the degree	1011:1020	the degree of deviation from the control (standard) indicators	1011:1072	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	1	64	theme	MS	240:241	arg1	investigation					136:148	investigation	136:148	investigation	136:148	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	1	64	theme	MS	240:241	arg1	purpose					111:117	The purpose	107:117	The purpose of the study	107:130	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	9	65	theme	natural	1901:1907	arg1	type					1909:1912	a natural type	1899:1912	a natural type of menopause	1899:1925	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	8	66	theme	chondroitin	1863:1873	arg1	sulfates					1875:1882	chondroitin sulfates	1863:1882	chondroitin sulfates	1863:1882	The arthropathic syndrome availability also confirms the significant reduction CI-6s / CI-4s ratio and an increased content of chondroitin sulfates.
28574385	0	67	with	PATIENTS	64:71	arg1	TYPES					86:90	VARIOUS TYPES	78:90	VARIOUS TYPES OF MENOPAUSE	78:103	[CONNECTIVE TISSUE METABOLIC FEATURES IN CLIMACTERIC SYNDROME'S PATIENTS WITH VARIOUS TYPES OF MENOPAUSE].
28574385	6	68	theme	dystrophic	1471:1480	arg1	changes					1482:1488	more severe dystrophic changes	1459:1488	more severe dystrophic changes	1459:1488	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	1	69	theme	tissue	168:173	arg1	investigation					136:148	investigation	136:148	investigation	136:148	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	1	69	theme	tissue	168:173	arg1	purpose					111:117	The purpose	107:117	The purpose of the study	107:130	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	9	70	theme	menopause	1917:1925	arg1	type					1909:1912	a natural type	1899:1912	a natural type of menopause	1899:1925	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	6	71	gly	glycoprotein	1253:1264	arg1	glycoprotein					1253:1264	glycoprotein	1253:1264	glycoprotein	1253:1264	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	7	72	theme	fractional	1551:1560	arg1	composition					1562:1572	the fractional composition	1547:1572	the fractional composition of glycosaminoglycans	1547:1594	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group indicates the presence of pre-emptive destruction of cartilage.
28574385	4	73	theme	quantitative	839:850	arg1	content					852:858	quantitative content	839:858	quantitative content	839:858	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	4	74	dep	content	852:858	arg1	the					835:837	the	835:837	the	835:837	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	4	75	dep	calculated	676:685	arg1	MMI					688:690	MMI	688:690	MMI	688:690	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	4	75	dep	calculated	676:685	arg1	assessed					737:744	assessed	737:744	was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC	733:832	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	4	76	theme	clinical-functional	799:817	arg1	index					819:823	the clinical-functional index	795:823	the clinical-functional index of WOMAC	795:832	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	1	77	theme	surgical	389:396	arg1	way					410:412	a surgical and natural way	387:412	a surgical and natural way	387:412	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	6	78	theme	clinical	1510:1517	arg1	symptoms					1519:1526	clinical symptoms	1510:1526	clinical symptoms	1510:1526	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	7	79	theme	destruction	1710:1720	arg1	presence					1686:1693	the presence	1682:1693	the presence of pre-emptive destruction of cartilage	1682:1733	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group indicates the presence of pre-emptive destruction of cartilage.
28574385	9	80	theme	connective	1961:1970	arg1	metabolites					1979:1989	connective tissue metabolites	1961:1989	connective tissue metabolites	1961:1989	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	1	81	theme	natural	402:408	arg1	way					410:412	a surgical and natural way	387:412	a surgical and natural way	387:412	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	8	82	theme	arthropathic	1740:1751	arg1	availability					1762:1773	The arthropathic syndrome availability	1736:1773	The arthropathic syndrome availability	1736:1773	The arthropathic syndrome availability also confirms the significant reduction CI-6s / CI-4s ratio and an increased content of chondroitin sulfates.
28574385	1	83	theme	study	126:130	arg1	investigation					136:148	investigation	136:148	investigation	136:148	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	1	83	theme	study	126:130	arg1	purpose					111:117	The purpose	107:117	The purpose of the study	107:130	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	9	84	dep	patients	2007:2014	arg1	contrast					1995:2002	contrast	1995:2002	contrast	1995:2002	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	4	85	theme	glycosaminoglycans	891:908	arg1	composition					876:886	qualitative composition	864:886	qualitative composition	864:886	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	4	85	theme	glycosaminoglycans	891:908	arg1	acids					975:979	uronic acids	968:979	uronic acids	968:979	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	4	85	theme	glycosaminoglycans	891:908	arg1	excretion					936:944	the urinary excretion	924:944	the urinary excretion of hydroxyproline	924:962	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	4	85	theme	glycosaminoglycans	891:908	arg1	content					852:858	quantitative content	839:858	quantitative content	839:858	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	6	86	theme	general	1305:1311	arg1	rearrangement					1325:1337	the general age-related rearrangement	1301:1337	the general age-related rearrangement of connective tissue metabolism	1301:1369	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	4	87	with	scale-questionnaire	751:769	arg1	calculation					780:790	the calculation	776:790	the calculation of the clinical-functional index of WOMAC	776:832	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	9	88	with	Patients	1885:1892	arg1	type					1909:1912	a natural type	1899:1912	a natural type of menopause	1899:1925	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	0	89	theme	MENOPAUSE	95:103	arg1	TYPES					86:90	VARIOUS TYPES	78:90	VARIOUS TYPES OF MENOPAUSE	78:103	[CONNECTIVE TISSUE METABOLIC FEATURES IN CLIMACTERIC SYNDROME'S PATIENTS WITH VARIOUS TYPES OF MENOPAUSE].
28574385	7	90	theme	group	1666:1670	arg1	patients					1645:1652	patients	1645:1652	patients of the main group	1645:1670	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group indicates the presence of pre-emptive destruction of cartilage.
28574385	5	91	dep	used	1078:1081	arg1	components					1147:1156	main components	1142:1156	main components method	1142:1163	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	1	92	theme	tissue	333:338	arg1	indicators					340:349	the connective tissue indicators	318:349	the connective tissue indicators	318:349	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	8	93	theme	significant	1793:1803	arg1	ratio					1829:1833	the significant reduction CI-6s / CI-4s ratio	1789:1833	the significant reduction CI-6s / CI-4s ratio	1789:1833	The arthropathic syndrome availability also confirms the significant reduction CI-6s / CI-4s ratio and an increased content of chondroitin sulfates.
28574385	6	94	contain	have	1201:1204	arg2	content					1219:1225	an increased content	1206:1225	an increased content of glycosaminoglycans and glycoprotein	1206:1264	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	6	94	contain	have	1201:1204	arg1	patients					1192:1199	all patients	1188:1199	all patients	1188:1199	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	0	95	theme	TISSUE	12:17	arg1	FEATURES					29:36	TISSUE METABOLIC FEATURES	12:36	TISSUE METABOLIC FEATURES IN CLIMACTERIC SYNDROME'S PATIENTS WITH VARIOUS TYPES OF MENOPAUSE	12:103	[CONNECTIVE TISSUE METABOLIC FEATURES IN CLIMACTERIC SYNDROME'S PATIENTS WITH VARIOUS TYPES OF MENOPAUSE].
28574385	8	96	theme	CI-6s	1815:1819	arg1	ratio					1829:1833	the significant reduction CI-6s / CI-4s ratio	1789:1833	the significant reduction CI-6s / CI-4s ratio	1789:1833	The arthropathic syndrome availability also confirms the significant reduction CI-6s / CI-4s ratio and an increased content of chondroitin sulfates.
28574385	5	97	theme	deviation	1025:1033	arg1	degree					1015:1020	the degree	1011:1020	the degree of deviation from the control (standard) indicators	1011:1072	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	4	98	theme	menopausal	655:664	arg1	index					666:670	The patients' modified menopausal index	632:670	The patients' modified menopausal index	632:670	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	6	99	contain	have	1407:1410	arg2	indictors					1427:1435	more expressed indictors	1412:1435	more expressed indictors	1412:1435	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	6	99	contain	have	1407:1410	arg1	patients					1380:1387	the patients	1376:1387	the patients of the main group	1376:1405	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	8	100	theme	CI-4s	1823:1827	arg1	ratio					1829:1833	the significant reduction CI-6s / CI-4s ratio	1789:1833	the significant reduction CI-6s / CI-4s ratio	1789:1833	The arthropathic syndrome availability also confirms the significant reduction CI-6s / CI-4s ratio and an increased content of chondroitin sulfates.
28574385	7	101	theme	sulfate	1634:1640	arg1	increase					1608:1615	the increase	1604:1615	the increase of chondroitin-6 sulfate in patients of the main group	1604:1670	Redistribution of the fractional composition of glycosaminoglycans towards the increase of chondroitin-6 sulfate in patients of the main group indicates the presence of pre-emptive destruction of cartilage.
28574385	6	102	theme	metabolism	1360:1369	arg1	rearrangement					1325:1337	the general age-related rearrangement	1301:1337	the general age-related rearrangement of connective tissue metabolism	1301:1369	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	1	103	theme	arthropathic	257:268	arg1	syndrome					270:277	the arthropathic syndrome	253:277	the arthropathic syndrome	253:277	The purpose of the study was investigation of the connective tissue metabolic features among women with menopausal syndrome (MS) and MS, in which the arthropathic syndrome is leading and comparative analysis of the connective tissue indicators exchange during menopause caused in a surgical and natural way.
28574385	3	104	theme	main	559:562	arg1	group					564:568	The main group	555:568	The main group	555:568	The main group included 40 women with MS with leading arthropathic syndrome.
28574385	6	105	theme	connective	1342:1351	arg1	metabolism					1360:1369	connective tissue metabolism	1342:1369	connective tissue metabolism	1342:1369	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	4	106	theme	urinary	928:934	arg1	excretion					936:944	the urinary excretion	924:944	the urinary excretion of hydroxyproline	924:962	The patients' modified menopausal index was calculated (MMI), the functional condition of the joints was assessed on a scale-questionnaire with the calculation of the clinical-functional index of WOMAC; the quantitative content and qualitative composition of glycosaminoglycans in the serum, the urinary excretion of hydroxyproline and uronic acids were determined.
28574385	5	107	theme	non-parametric	1083:1096	arg1	criterion					1111:1119	non-parametric Mann-Whitney criterion	1083:1119	non-parametric Mann-Whitney criterion	1083:1119	To determine the degree of deviation from the control (standard) indicators was used non-parametric Mann-Whitney criterion and factor analysis (main components method).
28574385	9	108	theme	pathologic	2082:2091	arg1	course					2093:2098	a longer and more invasive pathologic course	2055:2098	a longer and more invasive pathologic course of natural menopause	2055:2119	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	9	109	dep	menopause	2030:2038	arg1	indicates					2045:2053	indicates	2045:2053	indicates a longer and more invasive pathologic course of natural menopause	2045:2119	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
28574385	6	110	theme	group	1401:1405	arg1	patients					1380:1387	the patients	1376:1387	the patients of the main group	1376:1405	It was revealed that all patients have an increased content of glycosaminoglycans and glycoprotein in the blood, which corresponds to the general age-related rearrangement of connective tissue metabolism, but the patients of the main group have more expressed indictors, which corresponds to more severe dystrophic changes and the severity of clinical symptoms.
28574385	9	111	with	patients	2007:2014	arg1	menopause					2030:2038	surgical menopause	2021:2038	surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause	2021:2119	Patients with a natural type of menopause showed more pronounced changes in connective tissue metabolites, in contrast to patients with surgical menopause еhat indicates a longer and more invasive pathologic course of natural menopause.
26252208	0	0	theme	Complex	66:72	arg1	Interplay					74:82	a Complex Interplay	64:82	a Complex Interplay of Factors	64:93	Analysis of a Modern Hybrid and an Ancient Sugarcane Implicates a Complex Interplay of Factors in Affecting Recalcitrance to Cellulosic Ethanol Production.
26252208	7	1	theme	fermentation	1525:1536	arg1	efficiency					1538:1547	fermentation efficiency	1525:1547	fermentation efficiency	1525:1547	The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency.
26252208	9	2	theme	single	1813:1818	arg1	trait					1820:1824	a single trait	1811:1824	a quantitative heritage rather than a single trait	1775:1824	Together, these data support the hypothesis that biomass enzymatic hydrolysis recalcitrance is governed by a quantitative heritage rather than a single trait.
26252208	6	3	with	genotypes	1292:1300	arg1	changes					1317:1323	extensive changes	1307:1323	extensive changes in plant morphology and culm anatomy	1307:1360	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	6	4	theme	strong	1036:1041	arg1	influence					1043:1051	the strong influence	1032:1051	the strong influence of the environment on lignin content and composition	1032:1104	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	7	5	theme	hydrolysis	1504:1513	arg1	yield					1515:1519	enzymatic hydrolysis yield	1494:1519	enzymatic hydrolysis yield	1494:1519	The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency.
26252208	6	6	from	influence	1043:1051	arg1	content					1082:1088	lignin content	1075:1088	lignin content	1075:1088	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	6	6	from	influence	1043:1051	arg1	composition					1094:1104	composition	1094:1104	composition	1094:1104	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	1	7	theme	Abundant	156:163	arg1	evidence					165:172	Abundant evidence	156:172	Abundant evidence	156:172	Abundant evidence exists to support a role for lignin as an important element in biomass recalcitrance.
26252208	1	8	theme	biomass	237:243	arg1	recalcitrance					245:257	biomass recalcitrance	237:257	biomass recalcitrance	237:257	Abundant evidence exists to support a role for lignin as an important element in biomass recalcitrance.
26252208	6	9	theme	plant	1328:1332	arg1	morphology					1334:1343	plant morphology	1328:1343	plant morphology	1328:1343	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	2	10	theme	independent	277:287	arg1	studies					289:295	several independent studies	269:295	several independent studies	269:295	However, several independent studies have also shown that factors apart from lignin are also relevant and overall, the relative importance of different recalcitrance traits remains in dispute.
26252208	7	11	theme	modern	1439:1444	arg1	genotype					1446:1453	the modern genotype	1435:1453	the modern genotype	1435:1453	The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency.
26252208	6	12	from	changes	1317:1323	arg1	morphology					1334:1343	plant morphology	1328:1343	plant morphology	1328:1343	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	6	12	from	changes	1317:1323	arg1	anatomy					1354:1360	culm anatomy	1349:1360	culm anatomy	1349:1360	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	4	13	theme	different	757:765	arg1	stages					767:772	different stages	757:772	different stages of development	757:787	In addition we also tracked alterations in these characteristics in internodes at different stages of development.
26252208	9	14	theme	quantitative	1777:1788	arg1	heritage					1790:1797	a quantitative heritage	1775:1797	a quantitative heritage rather than a single trait	1775:1824	Together, these data support the hypothesis that biomass enzymatic hydrolysis recalcitrance is governed by a quantitative heritage rather than a single trait.
26252208	2	15	theme	several	269:275	arg1	studies					289:295	several independent studies	269:295	several independent studies	269:295	However, several independent studies have also shown that factors apart from lignin are also relevant and overall, the relative importance of different recalcitrance traits remains in dispute.
26252208	6	16	from	recalcitrance	1199:1211	arg1	biosynthesis					1235:1246	lignin biosynthesis	1228:1246	lignin biosynthesis	1228:1246	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	7	17	theme	enzymatic	1494:1502	arg1	yield					1515:1519	enzymatic hydrolysis yield	1494:1519	enzymatic hydrolysis yield	1494:1519	The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency.
26252208	9	18	theme	hydrolysis	1735:1744	arg1	recalcitrance					1746:1758	biomass enzymatic hydrolysis recalcitrance	1717:1758	biomass enzymatic hydrolysis recalcitrance	1717:1758	Together, these data support the hypothesis that biomass enzymatic hydrolysis recalcitrance is governed by a quantitative heritage rather than a single trait.
26252208	4	19	theme	development	777:787	arg1	stages					767:772	different stages	757:772	different stages of development	757:787	In addition we also tracked alterations in these characteristics in internodes at different stages of development.
26252208	7	20	theme	S/G	1386:1388	arg1	higher					1401:1406	higher	1401:1406	higher	1401:1406	The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency.
26252208	7	20	theme	S/G	1386:1388	arg1	ratio					1391:1395	The syringyl/guaiacyl (S/G) ratio	1363:1395	The syringyl/guaiacyl (S/G) ratio	1363:1395	The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency.
26252208	0	21	theme	Factors	87:93	arg1	Interplay					74:82	a Complex Interplay	64:82	a Complex Interplay of Factors	64:93	Analysis of a Modern Hybrid and an Ancient Sugarcane Implicates a Complex Interplay of Factors in Affecting Recalcitrance to Cellulosic Ethanol Production.
26252208	5	22	theme	lignin	924:929	arg1	content					931:937	lignin content	924:937	lignin content	924:937	Significant differences in the development of the culm between the genotypes were associated with clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield.
26252208	6	23	theme	extensive	1307:1315	arg1	changes					1317:1323	extensive changes	1307:1323	extensive changes in plant morphology and culm anatomy	1307:1360	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	7	24	theme	S/G	1460:1462	arg1	ratio					1464:1468	S/G ratio	1460:1468	S/G ratio	1460:1468	The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency.
26252208	6	25	from	changes	1217:1223	arg1	biosynthesis					1235:1246	lignin biosynthesis	1228:1246	lignin biosynthesis	1228:1246	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	8	26	theme	strong	1574:1579	arg1	correlation					1590:1600	a strong positive correlation	1572:1600	a strong positive correlation between ferulate ester level and cellulose conversion efficiency	1572:1665	Curiously we observed a strong positive correlation between ferulate ester level and cellulose conversion efficiency.
26252208	7	27	theme	genotype	1446:1453	arg1	internode					1422:1430	the oldest internode	1411:1430	the oldest internode of the modern genotype	1411:1453	The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency.
26252208	1	28	from	element	226:232	arg1	recalcitrance					245:257	biomass recalcitrance	237:257	biomass recalcitrance	237:257	Abundant evidence exists to support a role for lignin as an important element in biomass recalcitrance.
26252208	7	29	theme	syringyl/guaiacyl	1367:1383	arg1	higher					1401:1406	higher	1401:1406	higher	1401:1406	The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency.
26252208	7	29	theme	syringyl/guaiacyl	1367:1383	arg1	ratio					1391:1395	The syringyl/guaiacyl (S/G) ratio	1363:1395	The syringyl/guaiacyl (S/G) ratio	1363:1395	The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency.
26252208	3	30	with	study	550:554	arg1	variation					565:573	the variation	561:573	the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes	561:672	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
26252208	3	31	from	variation	565:573	arg1	parameters					589:598	anatomical parameters	578:598	anatomical parameters	578:598	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
26252208	3	31	from	variation	565:573	arg1	factors					642:648	recalcitrance factors	628:648	recalcitrance factors	628:648	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
26252208	3	31	from	variation	565:573	arg1	composition					611:621	cell wall composition	601:621	cell wall composition	601:621	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
26252208	5	32	theme	clear	888:892	arg1	distributions					907:919	clear differential distributions	888:919	clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield	888:1023	Significant differences in the development of the culm between the genotypes were associated with clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield.
26252208	0	33	theme	Modern	14:19	arg1	Hybrid					21:26	a Modern Hybrid	12:26	a Modern Hybrid	12:26	Analysis of a Modern Hybrid and an Ancient Sugarcane Implicates a Complex Interplay of Factors in Affecting Recalcitrance to Cellulosic Ethanol Production.
26252208	4	34	from	characteristics	724:738	arg1	internodes					743:752	internodes	743:752	internodes	743:752	In addition we also tracked alterations in these characteristics in internodes at different stages of development.
26252208	3	35	theme	correlational	536:548	arg1	study					550:554	a correlational study	534:554	a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes	534:672	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
26252208	6	36	theme	different	1282:1290	arg1	genotypes					1292:1300	different genotypes	1282:1300	different genotypes with extensive changes in plant morphology and culm anatomy	1282:1360	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	2	37	theme	different	402:410	arg1	traits					426:431	different recalcitrance traits	402:431	different recalcitrance traits	402:431	However, several independent studies have also shown that factors apart from lignin are also relevant and overall, the relative importance of different recalcitrance traits remains in dispute.
26252208	2	38	theme	recalcitrance	412:424	arg1	traits					426:431	different recalcitrance traits	402:431	different recalcitrance traits	402:431	However, several independent studies have also shown that factors apart from lignin are also relevant and overall, the relative importance of different recalcitrance traits remains in dispute.
26252208	8	39	theme	ester	1619:1623	arg1	level					1625:1629	ferulate ester level	1610:1629	ferulate ester level	1610:1629	Curiously we observed a strong positive correlation between ferulate ester level and cellulose conversion efficiency.
26252208	5	40	theme	fermentation	1006:1017	arg1	yield					1019:1023	fermentation yield	1006:1023	fermentation yield	1006:1023	Significant differences in the development of the culm between the genotypes were associated with clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield.
26252208	3	41	theme	recalcitrance	628:640	arg1	factors					642:648	recalcitrance factors	628:648	recalcitrance factors	628:648	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
26252208	3	42	theme	distant	491:497	arg1	genotypes					509:517	two genetically distant sugarcane genotypes	475:517	two genetically distant sugarcane genotypes	475:517	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
26252208	5	43	theme	differential	894:905	arg1	distributions					907:919	clear differential distributions	888:919	clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield	888:1023	Significant differences in the development of the culm between the genotypes were associated with clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield.
26252208	8	44	theme	ferulate	1610:1617	arg1	level					1625:1629	ferulate ester level	1610:1629	ferulate ester level	1610:1629	Curiously we observed a strong positive correlation between ferulate ester level and cellulose conversion efficiency.
26252208	0	45	theme	Cellulosic	125:134	arg1	Production					144:153	Cellulosic Ethanol Production	125:153	Cellulosic Ethanol Production	125:153	Analysis of a Modern Hybrid and an Ancient Sugarcane Implicates a Complex Interplay of Factors in Affecting Recalcitrance to Cellulosic Ethanol Production.
26252208	3	46	theme	anatomical	578:587	arg1	parameters					589:598	anatomical parameters	578:598	anatomical parameters	578:598	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
26252208	6	47	theme	lignin	1075:1080	arg1	content					1082:1088	lignin content	1075:1088	lignin content	1075:1088	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	0	48	theme	Hybrid	21:26	arg1	Analysis					0:7	Analysis	0:7	Analysis of a Modern Hybrid and an Ancient Sugarcane	0:51	Analysis of a Modern Hybrid and an Ancient Sugarcane Implicates a Complex Interplay of Factors in Affecting Recalcitrance to Cellulosic Ethanol Production.
26252208	5	49	theme	Significant	790:800	arg1	differences					802:812	Significant differences	790:812	Significant differences in the development of the culm between the genotypes	790:865	Significant differences in the development of the culm between the genotypes were associated with clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield.
26252208	2	50	theme	traits	426:431	arg1	importance					388:397	the relative importance	375:397	the relative importance of different recalcitrance traits	375:431	However, several independent studies have also shown that factors apart from lignin are also relevant and overall, the relative importance of different recalcitrance traits remains in dispute.
26252208	7	51	theme	oldest	1415:1420	arg1	internode					1422:1430	the oldest internode	1411:1430	the oldest internode of the modern genotype	1411:1453	The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency.
26252208	2	52	theme	relative	379:386	arg1	importance					388:397	the relative importance	375:397	the relative importance of different recalcitrance traits	375:431	However, several independent studies have also shown that factors apart from lignin are also relevant and overall, the relative importance of different recalcitrance traits remains in dispute.
26252208	0	53	theme	Ancient	35:41	arg1	Sugarcane					43:51	an Ancient Sugarcane	32:51	an Ancient Sugarcane	32:51	Analysis of a Modern Hybrid and an Ancient Sugarcane Implicates a Complex Interplay of Factors in Affecting Recalcitrance to Cellulosic Ethanol Production.
26252208	8	54	theme	positive	1581:1588	arg1	correlation					1590:1600	a strong positive correlation	1572:1600	a strong positive correlation between ferulate ester level and cellulose conversion efficiency	1572:1665	Curiously we observed a strong positive correlation between ferulate ester level and cellulose conversion efficiency.
26252208	5	55	theme	content	931:937	arg1	distributions					907:919	clear differential distributions	888:919	clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield	888:1023	Significant differences in the development of the culm between the genotypes were associated with clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield.
26252208	8	56	theme	conversion	1645:1654	arg1	efficiency					1656:1665	cellulose conversion efficiency	1635:1665	cellulose conversion efficiency	1635:1665	Curiously we observed a strong positive correlation between ferulate ester level and cellulose conversion efficiency.
26252208	0	57	theme	Ethanol	136:142	arg1	Production					144:153	Cellulosic Ethanol Production	125:153	Cellulosic Ethanol Production	125:153	Analysis of a Modern Hybrid and an Ancient Sugarcane Implicates a Complex Interplay of Factors in Affecting Recalcitrance to Cellulosic Ethanol Production.
26252208	3	58	used	used	470:473	arg2	we					467:468	we	467:468	we	467:468	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
26252208	9	59	theme	enzymatic	1725:1733	arg1	recalcitrance					1746:1758	biomass enzymatic hydrolysis recalcitrance	1717:1758	biomass enzymatic hydrolysis recalcitrance	1717:1758	Together, these data support the hypothesis that biomass enzymatic hydrolysis recalcitrance is governed by a quantitative heritage rather than a single trait.
26252208	9	60	theme	biomass	1717:1723	arg1	recalcitrance					1746:1758	biomass enzymatic hydrolysis recalcitrance	1717:1758	biomass enzymatic hydrolysis recalcitrance	1717:1758	Together, these data support the hypothesis that biomass enzymatic hydrolysis recalcitrance is governed by a quantitative heritage rather than a single trait.
26252208	8	61	theme	cellulose	1635:1643	arg1	efficiency					1656:1665	cellulose conversion efficiency	1635:1665	cellulose conversion efficiency	1635:1665	Curiously we observed a strong positive correlation between ferulate ester level and cellulose conversion efficiency.
26252208	3	62	theme	wall	606:609	arg1	composition					611:621	cell wall composition	601:621	cell wall composition	601:621	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
26252208	5	63	theme	composition	943:953	arg1	distributions					907:919	clear differential distributions	888:919	clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield	888:1023	Significant differences in the development of the culm between the genotypes were associated with clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield.
26252208	6	64	theme	environment	1060:1070	arg1	influence					1043:1051	the strong influence	1032:1051	the strong influence of the environment on lignin content and composition	1032:1104	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	6	65	theme	culm	1349:1352	arg1	anatomy					1354:1360	culm anatomy	1349:1360	culm anatomy	1349:1360	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	6	66	theme	single	1146:1151	arg1	plant					1153:1157	a single plant	1144:1157	a single plant	1144:1157	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	4	67	from	alterations	703:713	arg1	characteristics					724:738	these characteristics	718:738	these characteristics in internodes	718:752	In addition we also tracked alterations in these characteristics in internodes at different stages of development.
26252208	6	68	theme	lignin	1228:1233	arg1	biosynthesis					1235:1246	lignin biosynthesis	1228:1246	lignin biosynthesis	1228:1246	Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy.
26252208	5	69	theme	culm	840:843	arg1	development					821:831	the development	817:831	the development of the culm	817:843	Significant differences in the development of the culm between the genotypes were associated with clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield.
26252208	3	70	theme	sugarcane	499:507	arg1	genotypes					509:517	two genetically distant sugarcane genotypes	475:517	two genetically distant sugarcane genotypes	475:517	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
26252208	1	71	theme	important	216:224	arg1	element					226:232	an important element	213:232	an important element in biomass recalcitrance	213:257	Abundant evidence exists to support a role for lignin as an important element in biomass recalcitrance.
26252208	0	72	theme	Sugarcane	43:51	arg1	Analysis					0:7	Analysis	0:7	Analysis of a Modern Hybrid and an Ancient Sugarcane	0:51	Analysis of a Modern Hybrid and an Ancient Sugarcane Implicates a Complex Interplay of Factors in Affecting Recalcitrance to Cellulosic Ethanol Production.
26252208	5	73	from	differences	802:812	arg1	development					821:831	the development	817:831	the development of the culm	817:843	Significant differences in the development of the culm between the genotypes were associated with clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield.
26252208	3	74	theme	cell	601:604	arg1	composition					611:621	cell wall composition	601:621	cell wall composition	601:621	In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes.
25319448	0	0	theme	cows	81:84	arg1	SCC					68:70	SCC	68:70	SCC	68:70	Staphylococcus aureus intramammary infection affects milk yield and SCC of dairy cows.
25319448	0	0	theme	cows	81:84	arg1	yield					58:62	milk yield	53:62	milk yield	53:62	Staphylococcus aureus intramammary infection affects milk yield and SCC of dairy cows.
25319448	5	1	theme	quarter	819:825	arg1	level					827:831	quarter level	819:831	quarter level	819:831	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	1	2	theme	prevalent	133:141	arg1	microorganism					154:166	the most prevalent infectious microorganism	124:166	the most prevalent infectious microorganism affecting dairy cattle worldwide	124:199	Staphylococcus aureus (S. aureus) is the most prevalent infectious microorganism affecting dairy cattle worldwide, and its pathogenic characteristics facilitate its spread in dairy herds.
25319448	1	2	theme	prevalent	133:141	arg1	aureus					102:107	Staphylococcus aureus	87:107	Staphylococcus aureus (S. aureus)	87:119	Staphylococcus aureus (S. aureus) is the most prevalent infectious microorganism affecting dairy cattle worldwide, and its pathogenic characteristics facilitate its spread in dairy herds.
25319448	0	3	theme	dairy	75:79	arg1	cows					81:84	dairy cows	75:84	dairy cows	75:84	Staphylococcus aureus intramammary infection affects milk yield and SCC of dairy cows.
25319448	1	4	theme	infectious	143:152	arg1	microorganism					154:166	the most prevalent infectious microorganism	124:166	the most prevalent infectious microorganism affecting dairy cattle worldwide	124:199	Staphylococcus aureus (S. aureus) is the most prevalent infectious microorganism affecting dairy cattle worldwide, and its pathogenic characteristics facilitate its spread in dairy herds.
25319448	1	4	theme	infectious	143:152	arg1	aureus					102:107	Staphylococcus aureus	87:107	Staphylococcus aureus (S. aureus)	87:119	Staphylococcus aureus (S. aureus) is the most prevalent infectious microorganism affecting dairy cattle worldwide, and its pathogenic characteristics facilitate its spread in dairy herds.
25319448	5	5	theme	dairy	920:924	arg1	herds					926:930	two dairy herds	916:930	two dairy herds	916:930	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	2	6	theme	intramammary	285:296	arg1	IMI					310:312	IMI	310:312	IMI	310:312	S. aureus intramammary infections (IMI) are mainly subclinical, and associated losses can exceed average herd losses where the pathogen is not isolated.
25319448	2	6	theme	intramammary	285:296	arg1	infections					298:307	intramammary infections	285:307	S. aureus intramammary infections (IMI)	275:313	S. aureus intramammary infections (IMI) are mainly subclinical, and associated losses can exceed average herd losses where the pathogen is not isolated.
25319448	4	7	theme	mammary	692:698	arg1	yield					713:717	mammary quarter milk yield	692:717	mammary quarter milk yield	692:717	The aim of this study was to investigate the effects of S. aureus subclinical mastitis on mammary quarter milk yield and composition.
25319448	1	8	theme	dairy	262:266	arg1	herds					268:272	dairy herds	262:272	dairy herds	262:272	Staphylococcus aureus (S. aureus) is the most prevalent infectious microorganism affecting dairy cattle worldwide, and its pathogenic characteristics facilitate its spread in dairy herds.
25319448	5	9	from	effects	762:768	arg1	composition					804:814	composition	804:814	composition	804:814	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	5	9	from	effects	762:768	arg1	yield					794:798	milk yield	789:798	milk yield	789:798	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	4	10	theme	milk	708:711	arg1	yield					713:717	mammary quarter milk yield	692:717	mammary quarter milk yield	692:717	The aim of this study was to investigate the effects of S. aureus subclinical mastitis on mammary quarter milk yield and composition.
25319448	2	11	theme	associated	343:352	arg1	losses					354:359	associated losses	343:359	associated losses	343:359	S. aureus intramammary infections (IMI) are mainly subclinical, and associated losses can exceed average herd losses where the pathogen is not isolated.
25319448	5	12	from	herds	926:930	arg1	comparison					845:854	a pairwise comparison	834:854	a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds	834:930	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	5	12	from	herds	926:930	arg1	quarters					893:900	infected and non-infected mammary quarters	859:900	infected and non-infected mammary quarters (n = 28) from two dairy herds	859:930	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	5	12	from	herds	926:930	arg1	n = 28					903:908	n = 28	903:908	n = 28	903:908	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	4	13	theme	aureus	661:666	arg1	mastitis					680:687	S. aureus subclinical mastitis	658:687	S. aureus subclinical mastitis	658:687	The aim of this study was to investigate the effects of S. aureus subclinical mastitis on mammary quarter milk yield and composition.
25319448	5	14	theme	pairwise	836:843	arg1	comparison					845:854	a pairwise comparison	834:854	a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds	834:930	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	5	15	theme	pathogen	777:784	arg1	effects					762:768	the effects	758:768	the effects of the pathogen on milk yield and composition	758:814	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	2	16	theme	S.	275:276	arg1	IMI					310:312	IMI	310:312	IMI	310:312	S. aureus intramammary infections (IMI) are mainly subclinical, and associated losses can exceed average herd losses where the pathogen is not isolated.
25319448	2	16	theme	S.	275:276	arg1	infections					298:307	intramammary infections	285:307	S. aureus intramammary infections (IMI)	275:313	S. aureus intramammary infections (IMI) are mainly subclinical, and associated losses can exceed average herd losses where the pathogen is not isolated.
25319448	2	17	theme	average	372:378	arg1	losses					385:390	average herd losses	372:390	average herd losses where the pathogen is not isolated	372:425	S. aureus intramammary infections (IMI) are mainly subclinical, and associated losses can exceed average herd losses where the pathogen is not isolated.
25319448	8	18	from	positioning	1319:1329	arg1	cows					1334:1337	cows	1334:1337	cows	1334:1337	Fat yield from infected quarters decreased, but losses due to the infection caused by S. aureus were not associated with quarter positioning in cows.
25319448	8	19	from	quarters	1214:1221	arg1	yield					1194:1198	Fat yield	1190:1198	Fat yield from infected quarters	1190:1221	Fat yield from infected quarters decreased, but losses due to the infection caused by S. aureus were not associated with quarter positioning in cows.
25319448	2	20	theme	herd	380:383	arg1	losses					385:390	average herd losses	372:390	average herd losses where the pathogen is not isolated	372:425	S. aureus intramammary infections (IMI) are mainly subclinical, and associated losses can exceed average herd losses where the pathogen is not isolated.
25319448	0	21	theme	aureus	15:20	arg1	infection					35:43	Staphylococcus aureus intramammary infection	0:43	Staphylococcus aureus intramammary infection	0:43	Staphylococcus aureus intramammary infection affects milk yield and SCC of dairy cows.
25319448	7	22	theme	aureus	1122:1127	arg1	IMI					1129:1131	S. aureus IMI	1119:1131	S. aureus IMI	1119:1131	S. aureus has increased somatic cell counts at quarter level; however, no effect of S. aureus IMI on milk lactose, fat, and protein contents was observed.
25319448	3	23	theme	composite	530:538	arg1	losses					545:550	cow composite milk losses	526:550	cow composite milk losses	526:550	However, the extent it affects milk composition at udder and quarter levels is still unknown, and cow composite milk losses may be underestimated due to the dilution effect.
25319448	1	24	theme	dairy	178:182	arg1	worldwide					191:199	dairy cattle worldwide	178:199	dairy cattle worldwide	178:199	Staphylococcus aureus (S. aureus) is the most prevalent infectious microorganism affecting dairy cattle worldwide, and its pathogenic characteristics facilitate its spread in dairy herds.
25319448	0	25	theme	Staphylococcus	0:13	arg1	infection					35:43	Staphylococcus aureus intramammary infection	0:43	Staphylococcus aureus intramammary infection	0:43	Staphylococcus aureus intramammary infection affects milk yield and SCC of dairy cows.
25319448	7	26	theme	milk	1136:1139	arg1	lactose					1141:1147	milk lactose	1136:1147	milk lactose	1136:1147	S. aureus has increased somatic cell counts at quarter level; however, no effect of S. aureus IMI on milk lactose, fat, and protein contents was observed.
25319448	3	27	theme	milk	540:543	arg1	losses					545:550	cow composite milk losses	526:550	cow composite milk losses	526:550	However, the extent it affects milk composition at udder and quarter levels is still unknown, and cow composite milk losses may be underestimated due to the dilution effect.
25319448	1	28	theme	cattle	184:189	arg1	worldwide					191:199	dairy cattle worldwide	178:199	dairy cattle worldwide	178:199	Staphylococcus aureus (S. aureus) is the most prevalent infectious microorganism affecting dairy cattle worldwide, and its pathogenic characteristics facilitate its spread in dairy herds.
25319448	7	29	theme	quarter	1082:1088	arg1	level					1090:1094	quarter level	1082:1094	quarter level	1082:1094	S. aureus has increased somatic cell counts at quarter level; however, no effect of S. aureus IMI on milk lactose, fat, and protein contents was observed.
25319448	4	30	theme	mastitis	680:687	arg1	effects					647:653	the effects	643:653	the effects of S. aureus subclinical mastitis on mammary quarter milk yield and composition	643:733	The aim of this study was to investigate the effects of S. aureus subclinical mastitis on mammary quarter milk yield and composition.
25319448	5	31	theme	infected	859:866	arg1	n = 28					903:908	n = 28	903:908	n = 28	903:908	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	5	31	theme	infected	859:866	arg1	quarters					893:900	infected and non-infected mammary quarters	859:900	infected and non-infected mammary quarters (n = 28) from two dairy herds	859:930	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	4	32	theme	quarter	700:706	arg1	yield					713:717	mammary quarter milk yield	692:717	mammary quarter milk yield	692:717	The aim of this study was to investigate the effects of S. aureus subclinical mastitis on mammary quarter milk yield and composition.
25319448	5	33	theme	quarters	893:900	arg1	comparison					845:854	a pairwise comparison	834:854	a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds	834:930	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	0	34	theme	intramammary	22:33	arg1	infection					35:43	Staphylococcus aureus intramammary infection	0:43	Staphylococcus aureus intramammary infection	0:43	Staphylococcus aureus intramammary infection affects milk yield and SCC of dairy cows.
25319448	5	35	theme	non-infected	872:883	arg1	n = 28					903:908	n = 28	903:908	n = 28	903:908	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	5	35	theme	non-infected	872:883	arg1	quarters					893:900	infected and non-infected mammary quarters	859:900	infected and non-infected mammary quarters (n = 28) from two dairy herds	859:930	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	3	36	theme	cow	526:528	arg1	losses					545:550	cow composite milk losses	526:550	cow composite milk losses	526:550	However, the extent it affects milk composition at udder and quarter levels is still unknown, and cow composite milk losses may be underestimated due to the dilution effect.
25319448	3	37	theme	quarter	489:495	arg1	levels					497:502	udder and quarter levels	479:502	udder and quarter levels	479:502	However, the extent it affects milk composition at udder and quarter levels is still unknown, and cow composite milk losses may be underestimated due to the dilution effect.
25319448	0	38	theme	milk	53:56	arg1	yield					58:62	milk yield	53:62	milk yield	53:62	Staphylococcus aureus intramammary infection affects milk yield and SCC of dairy cows.
25319448	8	39	theme	infected	1205:1212	arg1	quarters					1214:1221	infected quarters	1205:1221	infected quarters	1205:1221	Fat yield from infected quarters decreased, but losses due to the infection caused by S. aureus were not associated with quarter positioning in cows.
25319448	1	40	from	spread	252:257	arg1	herds					268:272	dairy herds	262:272	dairy herds	262:272	Staphylococcus aureus (S. aureus) is the most prevalent infectious microorganism affecting dairy cattle worldwide, and its pathogenic characteristics facilitate its spread in dairy herds.
25319448	8	41	theme	quarter	1311:1317	arg1	positioning					1319:1329	quarter positioning	1311:1329	quarter positioning in cows	1311:1337	Fat yield from infected quarters decreased, but losses due to the infection caused by S. aureus were not associated with quarter positioning in cows.
25319448	4	42	theme	study	618:622	arg1	aim					606:608	The aim	602:608	The aim of this study	602:622	The aim of this study was to investigate the effects of S. aureus subclinical mastitis on mammary quarter milk yield and composition.
25319448	6	43	theme	milk	988:991	arg1	production					993:1002	milk production	988:1002	milk production	988:1002	Quarters were individually milked, and milk production and composition were assessed.
25319448	7	44	from	effect	1109:1114	arg1	fat					1150:1152	fat	1150:1152	fat	1150:1152	S. aureus has increased somatic cell counts at quarter level; however, no effect of S. aureus IMI on milk lactose, fat, and protein contents was observed.
25319448	7	44	from	effect	1109:1114	arg1	lactose					1141:1147	milk lactose	1136:1147	milk lactose	1136:1147	S. aureus has increased somatic cell counts at quarter level; however, no effect of S. aureus IMI on milk lactose, fat, and protein contents was observed.
25319448	7	44	from	effect	1109:1114	arg1	contents					1167:1174	protein contents	1159:1174	protein contents	1159:1174	S. aureus has increased somatic cell counts at quarter level; however, no effect of S. aureus IMI on milk lactose, fat, and protein contents was observed.
25319448	5	45	theme	milk	789:792	arg1	yield					794:798	milk yield	789:798	milk yield	789:798	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	7	46	theme	somatic	1059:1065	arg1	counts					1072:1077	somatic cell counts	1059:1077	somatic cell counts	1059:1077	S. aureus has increased somatic cell counts at quarter level; however, no effect of S. aureus IMI on milk lactose, fat, and protein contents was observed.
25319448	3	47	theme	udder	479:483	arg1	levels					497:502	udder and quarter levels	479:502	udder and quarter levels	479:502	However, the extent it affects milk composition at udder and quarter levels is still unknown, and cow composite milk losses may be underestimated due to the dilution effect.
25319448	8	48	theme	due	1245:1247	arg1	losses					1238:1243	losses	1238:1243	losses due to the infection caused by S. aureus	1238:1284	Fat yield from infected quarters decreased, but losses due to the infection caused by S. aureus were not associated with quarter positioning in cows.
25319448	4	49	theme	S.	658:659	arg1	mastitis					680:687	S. aureus subclinical mastitis	658:687	S. aureus subclinical mastitis	658:687	The aim of this study was to investigate the effects of S. aureus subclinical mastitis on mammary quarter milk yield and composition.
25319448	7	50	theme	cell	1067:1070	arg1	counts					1072:1077	somatic cell counts	1059:1077	somatic cell counts	1059:1077	S. aureus has increased somatic cell counts at quarter level; however, no effect of S. aureus IMI on milk lactose, fat, and protein contents was observed.
25319448	7	51	theme	protein	1159:1165	arg1	contents					1167:1174	protein contents	1159:1174	protein contents	1159:1174	S. aureus has increased somatic cell counts at quarter level; however, no effect of S. aureus IMI on milk lactose, fat, and protein contents was observed.
25319448	2	52	dep	infections	298:307	arg1	aureus					278:283	S. aureus intramammary infections (IMI)	275:313	S. aureus intramammary infections (IMI)	275:313	S. aureus intramammary infections (IMI) are mainly subclinical, and associated losses can exceed average herd losses where the pathogen is not isolated.
25319448	5	53	from	comparison	845:854	arg1	herds					926:930	two dairy herds	916:930	two dairy herds	916:930	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	4	54	theme	subclinical	668:678	arg1	mastitis					680:687	S. aureus subclinical mastitis	658:687	S. aureus subclinical mastitis	658:687	The aim of this study was to investigate the effects of S. aureus subclinical mastitis on mammary quarter milk yield and composition.
25319448	5	55	theme	mammary	885:891	arg1	n = 28					903:908	n = 28	903:908	n = 28	903:908	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	5	55	theme	mammary	885:891	arg1	quarters					893:900	infected and non-infected mammary quarters	859:900	infected and non-infected mammary quarters (n = 28) from two dairy herds	859:930	In order to determine the effects of the pathogen on milk yield and composition at quarter level, a pairwise comparison of infected and non-infected mammary quarters (n = 28) from two dairy herds was carried out.
25319448	1	56	theme	pathogenic	210:219	arg1	characteristics					221:235	its pathogenic characteristics	206:235	its pathogenic characteristics	206:235	Staphylococcus aureus (S. aureus) is the most prevalent infectious microorganism affecting dairy cattle worldwide, and its pathogenic characteristics facilitate its spread in dairy herds.
25319448	7	57	theme	S.	1119:1120	arg1	IMI					1129:1131	S. aureus IMI	1119:1131	S. aureus IMI	1119:1131	S. aureus has increased somatic cell counts at quarter level; however, no effect of S. aureus IMI on milk lactose, fat, and protein contents was observed.
25319448	3	58	theme	dilution	585:592	arg1	effect					594:599	the dilution effect	581:599	the dilution effect	581:599	However, the extent it affects milk composition at udder and quarter levels is still unknown, and cow composite milk losses may be underestimated due to the dilution effect.
25319448	3	59	theme	milk	459:462	arg1	composition					464:474	milk composition	459:474	milk composition	459:474	However, the extent it affects milk composition at udder and quarter levels is still unknown, and cow composite milk losses may be underestimated due to the dilution effect.
25319448	8	60	theme	Fat	1190:1192	arg1	yield					1194:1198	Fat yield	1190:1198	Fat yield from infected quarters	1190:1221	Fat yield from infected quarters decreased, but losses due to the infection caused by S. aureus were not associated with quarter positioning in cows.
25319448	7	61	theme	IMI	1129:1131	arg1	effect					1109:1114	no effect	1106:1114	no effect of S. aureus IMI on milk lactose, fat, and protein contents	1106:1174	S. aureus has increased somatic cell counts at quarter level; however, no effect of S. aureus IMI on milk lactose, fat, and protein contents was observed.
25319448	3	62	dep	extent	441:446	arg1	affects					451:457	affects	451:457	affects milk composition at udder and quarter levels	451:502	However, the extent it affects milk composition at udder and quarter levels is still unknown, and cow composite milk losses may be underestimated due to the dilution effect.
25319448	1	63	dep	aureus	102:107	arg1	aureus					113:118	S. aureus	110:118	S. aureus	110:118	Staphylococcus aureus (S. aureus) is the most prevalent infectious microorganism affecting dairy cattle worldwide, and its pathogenic characteristics facilitate its spread in dairy herds.
25319448	4	64	from	effects	647:653	arg1	yield					713:717	mammary quarter milk yield	692:717	mammary quarter milk yield	692:717	The aim of this study was to investigate the effects of S. aureus subclinical mastitis on mammary quarter milk yield and composition.
25319448	4	64	from	effects	647:653	arg1	composition					723:733	composition	723:733	composition	723:733	The aim of this study was to investigate the effects of S. aureus subclinical mastitis on mammary quarter milk yield and composition.
26796598	0	0	theme	tissue	86:91	arg1	applications					105:116	respiratory tissue engineering applications	74:116	respiratory tissue engineering applications	74:116	Electrospun nanofibers of poly(ε-caprolactone)/depolymerized chitosan for respiratory tissue engineering applications.
26796598	7	1	theme	biological	1172:1181	arg1	potential					1183:1191	biological potential	1172:1191	biological potential	1172:1191	To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30.
26796598	5	2	theme	chemical	938:945	arg1	analysis					957:964	chemical structure analysis	938:964	chemical structure analysis	938:964	Morphology and chemical structure analysis were performed to confirm the structure and composition of the fibers.
26796598	1	3	theme	damaged	339:345	arg1	system					354:359	a severely damaged airway system	328:359	a severely damaged airway system	328:359	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	0	4	theme	respiratory	74:84	arg1	applications					105:116	respiratory tissue engineering applications	74:116	respiratory tissue engineering applications	74:116	Electrospun nanofibers of poly(ε-caprolactone)/depolymerized chitosan for respiratory tissue engineering applications.
26796598	5	5	theme	structure	947:955	arg1	analysis					957:964	chemical structure analysis	938:964	chemical structure analysis	938:964	Morphology and chemical structure analysis were performed to confirm the structure and composition of the fibers.
26796598	3	6	theme	fibers	700:705	arg1	properties					682:691	the properties	678:691	the properties of the fibers for its potential use as a scaffold for regenerating tracheal tissue	678:774	In this research, we developed composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan using the electrospinning technique and assessed the properties of the fibers for its potential use as a scaffold for regenerating tracheal tissue.
26796598	10	7	contain	has	1724:1726	arg1	nanofiber					1714:1722	PCL/chitosan nanofiber	1701:1722	PCL/chitosan nanofiber	1701:1722	This study shows that PCL/chitosan nanofiber has sufficient structural integrity and serves as a potential candidate for tracheobronchial tissue engineering.
26796598	10	7	contain	has	1724:1726	arg2	integrity					1750:1758	sufficient structural integrity	1728:1758	sufficient structural integrity	1728:1758	This study shows that PCL/chitosan nanofiber has sufficient structural integrity and serves as a potential candidate for tracheobronchial tissue engineering.
26796598	10	7	contain	has	1724:1726	arg1	candidate					1786:1794	a potential candidate	1774:1794	a potential candidate for tracheobronchial tissue engineering	1774:1834	This study shows that PCL/chitosan nanofiber has sufficient structural integrity and serves as a potential candidate for tracheobronchial tissue engineering.
26796598	7	8	dep	assess	1165:1170	arg1	70/30					1339:1343	70/30	1339:1343	70/30	1339:1343	To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30.
26796598	7	8	dep	assess	1165:1170	arg1	90/10					1321:1325	90/10	1321:1325	90/10	1321:1325	To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30.
26796598	7	8	dep	assess	1165:1170	arg1	100/0					1314:1318	100/0	1314:1318	100/0	1314:1318	To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30.
26796598	7	8	dep	assess	1165:1170	arg1	80/20					1328:1332	80/20	1328:1332	80/20	1328:1332	To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30.
26796598	9	9	theme	time	1593:1596	arg1	points					1598:1603	different time points	1583:1603	different time points	1583:1603	Lactase dehydrogenase assay was carried out at different time points to determine cytotoxicity levels within PTBE cell cultures on nanofibers.
26796598	3	10	theme	composite	555:563	arg1	nanofibers					565:574	composite nanofibers	555:574	composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan	555:627	In this research, we developed composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan using the electrospinning technique and assessed the properties of the fibers for its potential use as a scaffold for regenerating tracheal tissue.
26796598	6	11	theme	Mechanical	1037:1046	arg1	testing					1048:1054	Mechanical testing	1037:1054	Mechanical testing of nanofibers	1037:1068	Mechanical testing of nanofibers demonstrated both elastic and ductile properties depending on the ratio of PCL to chitosan.
26796598	0	12	theme	engineering	93:103	arg1	applications					105:116	respiratory tissue engineering applications	74:116	respiratory tissue engineering applications	74:116	Electrospun nanofibers of poly(ε-caprolactone)/depolymerized chitosan for respiratory tissue engineering applications.
26796598	1	13	dep	structure	302:310	arg1	the					298:300	the	298:300	the	298:300	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	8	14	theme	nanofiber	1387:1395	arg1	membrane					1397:1404	the nanofiber membrane	1383:1404	the nanofiber membrane	1383:1404	Transwell inserts were modified with the nanofiber membrane and cells were seeded according to air-liquid interface culture techniques that mimics the conditions found in the human airways.
26796598	7	15	theme	PCL/chitosan	1300:1311	arg1	ratios					1290:1295	composition ratios	1278:1295	composition ratios of PCL/chitosan	1278:1311	To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30.
26796598	10	16	theme	potential	1776:1784	arg1	nanofiber					1714:1722	PCL/chitosan nanofiber	1701:1722	PCL/chitosan nanofiber	1701:1722	This study shows that PCL/chitosan nanofiber has sufficient structural integrity and serves as a potential candidate for tracheobronchial tissue engineering.
26796598	10	16	theme	potential	1776:1784	arg1	candidate					1786:1794	a potential candidate	1774:1794	a potential candidate for tracheobronchial tissue engineering	1774:1834	This study shows that PCL/chitosan nanofiber has sufficient structural integrity and serves as a potential candidate for tracheobronchial tissue engineering.
26796598	6	17	theme	nanofibers	1059:1068	arg1	testing					1048:1054	Mechanical testing	1037:1054	Mechanical testing of nanofibers	1037:1068	Mechanical testing of nanofibers demonstrated both elastic and ductile properties depending on the ratio of PCL to chitosan.
26796598	1	18	theme	airway	347:352	arg1	system					354:359	a severely damaged airway system	328:359	a severely damaged airway system	328:359	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	1	19	theme	natural	196:202	arg1	tree					221:224	the natural tracheobronchial tree	192:224	the natural tracheobronchial tree	192:224	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	4	20	theme	depolymerized	791:803	arg1	solution					814:821	Water-soluble depolymerized chitosan solution	777:821	Water-soluble depolymerized chitosan solution	777:821	Water-soluble depolymerized chitosan solution was first prepared and mixed with polycaprolactone solution making it suitable for electrospinning.
26796598	2	21	theme	mechanical	501:510	arg1	properties					512:521	sufficient mechanical properties	490:521	sufficient mechanical properties	490:521	For this specific application, the selected scaffold material should be biocompatible, elicit limited cytotoxicity, and exhibit sufficient mechanical properties.
26796598	1	22	theme	system	354:359	arg1	function					316:323	function	316:323	function	316:323	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	1	22	theme	system	354:359	arg1	structure					302:310	structure	302:310	structure	302:310	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	4	23	theme	polycaprolactone	857:872	arg1	solution					874:881	polycaprolactone solution	857:881	polycaprolactone solution	857:881	Water-soluble depolymerized chitosan solution was first prepared and mixed with polycaprolactone solution making it suitable for electrospinning.
26796598	4	24	theme	Water-soluble	777:789	arg1	solution					814:821	Water-soluble depolymerized chitosan solution	777:821	Water-soluble depolymerized chitosan solution	777:821	Water-soluble depolymerized chitosan solution was first prepared and mixed with polycaprolactone solution making it suitable for electrospinning.
26796598	6	25	theme	PCL	1145:1147	arg1	ratio					1136:1140	the ratio	1132:1140	the ratio of PCL to chitosan	1132:1159	Mechanical testing of nanofibers demonstrated both elastic and ductile properties depending on the ratio of PCL to chitosan.
26796598	2	26	theme	specific	371:378	arg1	application					380:390	this specific application	366:390	this specific application	366:390	For this specific application, the selected scaffold material should be biocompatible, elicit limited cytotoxicity, and exhibit sufficient mechanical properties.
26796598	8	27	theme	culture	1462:1468	arg1	techniques					1470:1479	air-liquid interface culture techniques	1441:1479	air-liquid interface culture techniques that mimics the conditions found in the human airways	1441:1533	Transwell inserts were modified with the nanofiber membrane and cells were seeded according to air-liquid interface culture techniques that mimics the conditions found in the human airways.
26796598	7	28	theme	composition	1278:1288	arg1	ratios					1290:1295	composition ratios	1278:1295	composition ratios of PCL/chitosan	1278:1311	To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30.
26796598	9	29	theme	cytotoxicity	1618:1629	arg1	levels					1631:1636	cytotoxicity levels	1618:1636	cytotoxicity levels within PTBE cell cultures on nanofibers	1618:1676	Lactase dehydrogenase assay was carried out at different time points to determine cytotoxicity levels within PTBE cell cultures on nanofibers.
26796598	10	30	theme	PCL/chitosan	1701:1712	arg1	nanofiber					1714:1722	PCL/chitosan nanofiber	1701:1722	PCL/chitosan nanofiber	1701:1722	This study shows that PCL/chitosan nanofiber has sufficient structural integrity and serves as a potential candidate for tracheobronchial tissue engineering.
26796598	10	30	theme	PCL/chitosan	1701:1712	arg1	candidate					1786:1794	a potential candidate	1774:1794	a potential candidate for tracheobronchial tissue engineering	1774:1834	This study shows that PCL/chitosan nanofiber has sufficient structural integrity and serves as a potential candidate for tracheobronchial tissue engineering.
26796598	0	31	theme	Electrospun	0:10	arg1	nanofibers					12:21	Electrospun nanofibers	0:21	Electrospun nanofibers of poly(ε-caprolactone)	0:45	Electrospun nanofibers of poly(ε-caprolactone)/depolymerized chitosan for respiratory tissue engineering applications.
26796598	3	32	theme	regenerating	747:758	arg1	tissue					769:774	regenerating tracheal tissue	747:774	regenerating tracheal tissue	747:774	In this research, we developed composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan using the electrospinning technique and assessed the properties of the fibers for its potential use as a scaffold for regenerating tracheal tissue.
26796598	0	33	theme	poly	26:29	arg1	nanofibers					12:21	Electrospun nanofibers	0:21	Electrospun nanofibers of poly(ε-caprolactone)	0:45	Electrospun nanofibers of poly(ε-caprolactone)/depolymerized chitosan for respiratory tissue engineering applications.
26796598	3	34	theme	tracheal	760:767	arg1	tissue					769:774	regenerating tracheal tissue	747:774	regenerating tracheal tissue	747:774	In this research, we developed composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan using the electrospinning technique and assessed the properties of the fibers for its potential use as a scaffold for regenerating tracheal tissue.
26796598	1	35	from	scaffold	158:165	arg1	shape					183:187	shape	183:187	shape	183:187	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	1	35	from	scaffold	158:165	arg1	size					174:177	size	174:177	size	174:177	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	10	36	theme	structural	1739:1748	arg1	integrity					1750:1758	sufficient structural integrity	1728:1758	sufficient structural integrity	1728:1758	This study shows that PCL/chitosan nanofiber has sufficient structural integrity and serves as a potential candidate for tracheobronchial tissue engineering.
26796598	2	37	theme	scaffold	406:413	arg1	material					415:422	the selected scaffold material	393:422	the selected scaffold material	393:422	For this specific application, the selected scaffold material should be biocompatible, elicit limited cytotoxicity, and exhibit sufficient mechanical properties.
26796598	2	37	theme	scaffold	406:413	arg1	biocompatible					434:446	biocompatible	434:446	biocompatible	434:446	For this specific application, the selected scaffold material should be biocompatible, elicit limited cytotoxicity, and exhibit sufficient mechanical properties.
26796598	7	38	theme	PTBE	1231:1234	arg1	cells					1237:1241	porcine tracheobronchial epithelial (PTBE) cells	1194:1241	porcine tracheobronchial epithelial (PTBE) cells	1194:1241	To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30.
26796598	10	39	theme	tissue	1817:1822	arg1	engineering					1824:1834	tracheobronchial tissue engineering	1800:1834	tracheobronchial tissue engineering	1800:1834	This study shows that PCL/chitosan nanofiber has sufficient structural integrity and serves as a potential candidate for tracheobronchial tissue engineering.
26796598	1	40	theme	Synthetic	119:127	arg1	grafts					129:134	Synthetic grafts	119:134	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree	119:224	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	9	41	theme	different	1583:1591	arg1	points					1598:1603	different time points	1583:1603	different time points	1583:1603	Lactase dehydrogenase assay was carried out at different time points to determine cytotoxicity levels within PTBE cell cultures on nanofibers.
26796598	2	42	theme	selected	397:404	arg1	material					415:422	the selected scaffold material	393:422	the selected scaffold material	393:422	For this specific application, the selected scaffold material should be biocompatible, elicit limited cytotoxicity, and exhibit sufficient mechanical properties.
26796598	2	42	theme	selected	397:404	arg1	biocompatible					434:446	biocompatible	434:446	biocompatible	434:446	For this specific application, the selected scaffold material should be biocompatible, elicit limited cytotoxicity, and exhibit sufficient mechanical properties.
26796598	7	43	theme	tracheobronchial	1202:1217	arg1	cells					1237:1241	porcine tracheobronchial epithelial (PTBE) cells	1194:1241	porcine tracheobronchial epithelial (PTBE) cells	1194:1241	To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30.
26796598	6	44	theme	ductile	1100:1106	arg1	properties					1108:1117	both elastic and ductile properties	1083:1117	both elastic and ductile properties	1083:1117	Mechanical testing of nanofibers demonstrated both elastic and ductile properties depending on the ratio of PCL to chitosan.
26796598	9	45	theme	cell	1650:1653	arg1	cultures					1655:1662	PTBE cell cultures	1645:1662	PTBE cell cultures on nanofibers	1645:1676	Lactase dehydrogenase assay was carried out at different time points to determine cytotoxicity levels within PTBE cell cultures on nanofibers.
26796598	9	46	from	cultures	1655:1662	arg1	nanofibers					1667:1676	nanofibers	1667:1676	nanofibers	1667:1676	Lactase dehydrogenase assay was carried out at different time points to determine cytotoxicity levels within PTBE cell cultures on nanofibers.
26796598	1	47	theme	tracheobronchial	204:219	arg1	tree					221:224	the natural tracheobronchial tree	192:224	the natural tracheobronchial tree	192:224	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	2	48	theme	limited	456:462	arg1	cytotoxicity					464:475	limited cytotoxicity	456:475	limited cytotoxicity	456:475	For this specific application, the selected scaffold material should be biocompatible, elicit limited cytotoxicity, and exhibit sufficient mechanical properties.
26796598	7	49	theme	epithelial	1219:1228	arg1	cells					1237:1241	porcine tracheobronchial epithelial (PTBE) cells	1194:1241	porcine tracheobronchial epithelial (PTBE) cells	1194:1241	To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30.
26796598	1	50	theme	tree	221:224	arg1	shape					183:187	shape	183:187	shape	183:187	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	1	50	theme	tree	221:224	arg1	size					174:177	size	174:177	size	174:177	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	3	51	theme	electrospinning	639:653	arg1	technique					655:663	the electrospinning technique	635:663	the electrospinning technique	635:663	In this research, we developed composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan using the electrospinning technique and assessed the properties of the fibers for its potential use as a scaffold for regenerating tracheal tissue.
26796598	3	52	theme	polycaprolactone	579:594	arg1	nanofibers					565:574	composite nanofibers	555:574	composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan	555:627	In this research, we developed composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan using the electrospinning technique and assessed the properties of the fibers for its potential use as a scaffold for regenerating tracheal tissue.
26796598	3	53	theme	potential	715:723	arg1	use					725:727	its potential use	711:727	its potential use as a scaffold for regenerating tracheal tissue	711:774	In this research, we developed composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan using the electrospinning technique and assessed the properties of the fibers for its potential use as a scaffold for regenerating tracheal tissue.
26796598	9	54	theme	Lactase	1536:1542	arg1	assay					1558:1562	Lactase dehydrogenase assay	1536:1562	Lactase dehydrogenase assay	1536:1562	Lactase dehydrogenase assay was carried out at different time points to determine cytotoxicity levels within PTBE cell cultures on nanofibers.
26796598	7	55	theme	porcine	1194:1200	arg1	cells					1237:1241	porcine tracheobronchial epithelial (PTBE) cells	1194:1241	porcine tracheobronchial epithelial (PTBE) cells	1194:1241	To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30.
26796598	8	56	theme	interface	1452:1460	arg1	techniques					1470:1479	air-liquid interface culture techniques	1441:1479	air-liquid interface culture techniques that mimics the conditions found in the human airways	1441:1533	Transwell inserts were modified with the nanofiber membrane and cells were seeded according to air-liquid interface culture techniques that mimics the conditions found in the human airways.
26796598	8	57	theme	human	1521:1525	arg1	airways					1527:1533	the human airways	1517:1533	the human airways	1517:1533	Transwell inserts were modified with the nanofiber membrane and cells were seeded according to air-liquid interface culture techniques that mimics the conditions found in the human airways.
26796598	8	58	theme	air-liquid	1441:1450	arg1	techniques					1470:1479	air-liquid interface culture techniques	1441:1479	air-liquid interface culture techniques that mimics the conditions found in the human airways	1441:1533	Transwell inserts were modified with the nanofiber membrane and cells were seeded according to air-liquid interface culture techniques that mimics the conditions found in the human airways.
26796598	9	59	theme	dehydrogenase	1544:1556	arg1	assay					1558:1562	Lactase dehydrogenase assay	1536:1562	Lactase dehydrogenase assay	1536:1562	Lactase dehydrogenase assay was carried out at different time points to determine cytotoxicity levels within PTBE cell cultures on nanofibers.
26796598	3	60	theme	chitosan	620:627	arg1	nanofibers					565:574	composite nanofibers	555:574	composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan	555:627	In this research, we developed composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan using the electrospinning technique and assessed the properties of the fibers for its potential use as a scaffold for regenerating tracheal tissue.
26796598	9	61	theme	PTBE	1645:1648	arg1	cultures					1655:1662	PTBE cell cultures	1645:1662	PTBE cell cultures on nanofibers	1645:1676	Lactase dehydrogenase assay was carried out at different time points to determine cytotoxicity levels within PTBE cell cultures on nanofibers.
26796598	6	62	theme	elastic	1088:1094	arg1	properties					1108:1117	both elastic and ductile properties	1083:1117	both elastic and ductile properties	1083:1117	Mechanical testing of nanofibers demonstrated both elastic and ductile properties depending on the ratio of PCL to chitosan.
26796598	5	63	theme	fibers	1029:1034	arg1	structure					996:1004	structure	996:1004	structure	996:1004	Morphology and chemical structure analysis were performed to confirm the structure and composition of the fibers.
26796598	5	63	theme	fibers	1029:1034	arg1	composition					1010:1020	composition	1010:1020	composition	1010:1020	Morphology and chemical structure analysis were performed to confirm the structure and composition of the fibers.
26796598	4	64	theme	chitosan	805:812	arg1	solution					814:821	Water-soluble depolymerized chitosan solution	777:821	Water-soluble depolymerized chitosan solution	777:821	Water-soluble depolymerized chitosan solution was first prepared and mixed with polycaprolactone solution making it suitable for electrospinning.
26796598	10	65	theme	tracheobronchial	1800:1815	arg1	engineering					1824:1834	tracheobronchial tissue engineering	1800:1834	tracheobronchial tissue engineering	1800:1834	This study shows that PCL/chitosan nanofiber has sufficient structural integrity and serves as a potential candidate for tracheobronchial tissue engineering.
26796598	8	66	located	found	1508:1512	arg1	airways					1527:1533	the human airways	1517:1533	the human airways	1517:1533	Transwell inserts were modified with the nanofiber membrane and cells were seeded according to air-liquid interface culture techniques that mimics the conditions found in the human airways.
26796598	8	66	located	found	1508:1512	arg2	conditions					1497:1506	the conditions	1493:1506	the conditions found in the human airways	1493:1533	Transwell inserts were modified with the nanofiber membrane and cells were seeded according to air-liquid interface culture techniques that mimics the conditions found in the human airways.
26796598	2	67	theme	sufficient	490:499	arg1	properties					512:521	sufficient mechanical properties	490:521	sufficient mechanical properties	490:521	For this specific application, the selected scaffold material should be biocompatible, elicit limited cytotoxicity, and exhibit sufficient mechanical properties.
26796598	10	68	theme	sufficient	1728:1737	arg1	integrity					1750:1758	sufficient structural integrity	1728:1758	sufficient structural integrity	1728:1758	This study shows that PCL/chitosan nanofiber has sufficient structural integrity and serves as a potential candidate for tracheobronchial tissue engineering.
26796598	1	69	theme	porous	151:156	arg1	scaffold					158:165	a porous scaffold	149:165	a porous scaffold in the size and shape of the natural tracheobronchial tree	149:224	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	8	70	mod	modified	1369:1376	arg1	inserts					1356:1362	Transwell inserts	1346:1362	Transwell inserts	1346:1362	Transwell inserts were modified with the nanofiber membrane and cells were seeded according to air-liquid interface culture techniques that mimics the conditions found in the human airways.
26796598	8	70	mod	modified	1369:1376	arg3	membrane					1397:1404	the nanofiber membrane	1383:1404	the nanofiber membrane	1383:1404	Transwell inserts were modified with the nanofiber membrane and cells were seeded according to air-liquid interface culture techniques that mimics the conditions found in the human airways.
26796598	1	71	theme	autologous	231:240	arg1	cells					247:251	autologous stem cells	231:251	autologous stem cells	231:251	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
26796598	3	72	theme	depolymerized	606:618	arg1	chitosan					620:627	depolymerized chitosan	606:627	depolymerized chitosan	606:627	In this research, we developed composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan using the electrospinning technique and assessed the properties of the fibers for its potential use as a scaffold for regenerating tracheal tissue.
26796598	8	73	theme	Transwell	1346:1354	arg1	inserts					1356:1362	Transwell inserts	1346:1362	Transwell inserts	1346:1362	Transwell inserts were modified with the nanofiber membrane and cells were seeded according to air-liquid interface culture techniques that mimics the conditions found in the human airways.
26796598	1	74	theme	stem	242:245	arg1	cells					247:251	autologous stem cells	231:251	autologous stem cells	231:251	Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system.
28372195	2	0	theme	sample	354:359	arg1	analysis					361:368	real honey sample analysis	343:368	real honey sample analysis	343:368	Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported.
28372195	6	1	theme	Diastase	1023:1030	arg1	activity					1032:1039	Diastase activity	1023:1039	Diastase activity	1023:1039	Diastase activity, sucrose content and hydroxymethylfurfural content were easily differentiated and these parameters were used for indication of the adulteration of the honey.
28372195	5	2	theme	artificial	903:912	arg1	feeding					914:920	the artificial feeding	899:920	the artificial feeding of honeybees with carbohydrate supplements	899:963	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	2	3	theme	honey	348:352	arg1	analysis					361:368	real honey sample analysis	343:368	real honey sample analysis	343:368	Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported.
28372195	0	4	theme	chemometric	70:80	arg1	analysis					82:89	a chemometric analysis	68:89	a chemometric analysis for identification of honey freshness and adulteration	68:144	HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration.
28372195	0	5	from	HMF	0:2	arg1	honeys					29:34	honeys	29:34	honeys	29:34	HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration.
28372195	4	6	theme	good	774:777	arg1	accordance					779:788	good accordance	774:788	good accordance with the certified values	774:814	Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values.
28372195	5	7	theme	chemical	973:980	arg1	composition					982:992	chemical composition	973:992	chemical composition	973:992	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	3	8	theme	detection	541:549	arg1	limits					531:536	The limits	527:536	The limits of detection and quantification	527:568	The limits of detection and quantification were calculated.
28372195	4	9	theme	reference	721:729	arg1	material					731:738	the certified reference material	707:738	the certified reference material	707:738	Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values.
28372195	2	10	theme	vast	385:388	arg1	number					390:395	the vast number	381:395	the vast number of collected data sets	381:418	Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported.
28372195	1	11	theme	validated	155:163	arg1	approach					165:172	A fully validated approach	147:172	A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys	147:259	A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods.
28372195	2	12	theme	quality	495:501	arg1	criteria					503:510	the quality criteria	491:510	the quality criteria	491:510	Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported.
28372195	4	13	theme	determined	748:757	arg1	values					759:764	the determined values	744:764	the determined values	744:764	Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values.
28372195	5	14	with	honeybees	925:933	arg1	supplements					953:963	carbohydrate supplements	940:963	carbohydrate supplements	940:963	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	6	15	theme	honey	1192:1196	arg1	adulteration					1172:1183	the adulteration	1168:1183	the adulteration of the honey	1168:1196	Diastase activity, sucrose content and hydroxymethylfurfural content were easily differentiated and these parameters were used for indication of the adulteration of the honey.
28372195	4	16	from	time	652:655	arg1	techniques					690:699	the kinetic and spectrometric techniques	660:699	the kinetic and spectrometric techniques using the certified reference material	660:738	Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values.
28372195	2	17	theme	due	374:376	arg1	conclusions					429:439	due to the vast number of collected data sets reliable conclusions	374:439	due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria	374:510	Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported.
28372195	2	18	theme	reliable	420:427	arg1	conclusions					429:439	due to the vast number of collected data sets reliable conclusions	374:439	due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria	374:510	Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported.
28372195	4	19	with	accordance	779:788	arg1	values					809:814	the certified values	795:814	the certified values	795:814	Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values.
28372195	2	20	theme	data	410:413	arg1	sets					415:418	collected data sets	400:418	collected data sets	400:418	Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported.
28372195	0	21	theme	diastase	8:15	arg1	activity					17:24	diastase activity	8:24	diastase activity	8:24	HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration.
28372195	3	22	theme	quantification	555:568	arg1	limits					531:536	The limits	527:536	The limits of detection and quantification	527:568	The limits of detection and quantification were calculated.
28372195	2	23	theme	collected	400:408	arg1	sets					415:418	collected data sets	400:418	collected data sets	400:418	Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported.
28372195	5	24	theme	honeybees	925:933	arg1	feeding					914:920	the artificial feeding	899:920	the artificial feeding of honeybees with carbohydrate supplements	899:963	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	5	24	theme	honeybees	925:933	arg1	properties					998:1007	properties	998:1007	properties	998:1007	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	5	24	theme	honeybees	925:933	arg1	composition					982:992	chemical composition	973:992	chemical composition	973:992	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	0	25	theme	freshness	119:127	arg1	identification					95:108	identification	95:108	identification of honey freshness and adulteration	95:144	HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration.
28372195	4	26	theme	kinetic	664:670	arg1	techniques					690:699	the kinetic and spectrometric techniques	660:699	the kinetic and spectrometric techniques using the certified reference material	660:738	Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values.
28372195	6	27	theme	hydroxymethylfurfural	1062:1082	arg1	content					1084:1090	hydroxymethylfurfural content	1062:1090	hydroxymethylfurfural content	1062:1090	Diastase activity, sucrose content and hydroxymethylfurfural content were easily differentiated and these parameters were used for indication of the adulteration of the honey.
28372195	1	28	theme	official	299:306	arg1	methods					308:314	the official methods	295:314	the official methods	295:314	A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods.
28372195	1	29	from	honeys	254:259	arg1	determination					182:194	the determination	178:194	the determination of diastase activity and hydroxymethylfurfural content in honeys	178:259	A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods.
28372195	5	30	theme	cluster	825:831	arg1	analysis					833:840	cluster analysis	825:840	cluster analysis	825:840	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	1	31	theme	diastase	199:206	arg1	activity					208:215	diastase activity	199:215	diastase activity	199:215	A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods.
28372195	0	32	theme	adulteration	133:144	arg1	identification					95:108	identification	95:108	identification of honey freshness and adulteration	95:144	HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration.
28372195	2	33	theme	real	343:346	arg1	analysis					361:368	real honey sample analysis	343:368	real honey sample analysis	343:368	Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported.
28372195	0	34	theme	honey	113:117	arg1	freshness					119:127	honey freshness	113:127	honey freshness	113:127	HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration.
28372195	1	35	from	content	243:249	arg1	honeys					254:259	honeys	254:259	honeys	254:259	A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods.
28372195	6	36	used	used	1145:1148	arg2	parameters					1129:1138	these parameters	1123:1138	these parameters	1123:1138	Diastase activity, sucrose content and hydroxymethylfurfural content were easily differentiated and these parameters were used for indication of the adulteration of the honey.
28372195	4	37	theme	certified	711:719	arg1	material					731:738	the certified reference material	707:738	the certified reference material	707:738	Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values.
28372195	1	38	from	activity	208:215	arg1	honeys					254:259	honeys	254:259	honeys	254:259	A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods.
28372195	4	39	theme	certified	799:807	arg1	values					809:814	the certified values	795:814	the certified values	795:814	Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values.
28372195	1	40	from	determination	182:194	arg1	honeys					254:259	honeys	254:259	honeys	254:259	A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods.
28372195	5	41	dep	composition	982:992	arg1	the					969:971	the	969:971	the	969:971	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	1	42	theme	activity	208:215	arg1	determination					182:194	the determination	178:194	the determination of diastase activity and hydroxymethylfurfural content in honeys	178:259	A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods.
28372195	5	43	theme	honey	1016:1020	arg1	feeding					914:920	the artificial feeding	899:920	the artificial feeding of honeybees with carbohydrate supplements	899:963	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	5	43	theme	honey	1016:1020	arg1	properties					998:1007	properties	998:1007	properties	998:1007	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	5	43	theme	honey	1016:1020	arg1	composition					982:992	chemical composition	973:992	chemical composition	973:992	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	5	44	theme	carbohydrate	940:951	arg1	supplements					953:963	carbohydrate supplements	940:963	carbohydrate supplements	940:963	PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey.
28372195	6	45	theme	adulteration	1172:1183	arg1	indication					1154:1163	indication	1154:1163	indication of the adulteration of the honey	1154:1196	Diastase activity, sucrose content and hydroxymethylfurfural content were easily differentiated and these parameters were used for indication of the adulteration of the honey.
28372195	6	46	theme	sucrose	1042:1048	arg1	content					1050:1056	sucrose content	1042:1056	sucrose content	1042:1056	Diastase activity, sucrose content and hydroxymethylfurfural content were easily differentiated and these parameters were used for indication of the adulteration of the honey.
28372195	0	47	theme	validated	45:53	arg1	approach					55:62	A fully validated approach	37:62	A fully validated approach	37:62	HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration.
28372195	4	48	theme	first	646:650	arg1	time					652:655	the first time	642:655	the first time in the kinetic and spectrometric techniques using the certified reference material	642:738	Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values.
28372195	2	49	theme	sets	415:418	arg1	number					390:395	the vast number	381:395	the vast number of collected data sets	381:418	Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported.
28372195	1	50	theme	hydroxymethylfurfural	221:241	arg1	content					243:249	hydroxymethylfurfural content	221:249	hydroxymethylfurfural content	221:249	A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods.
28372195	0	51	from	activity	17:24	arg1	honeys					29:34	honeys	29:34	honeys	29:34	HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration.
28372195	0	52	dep	HMF	0:2	arg1	approach					55:62	A fully validated approach	37:62	A fully validated approach	37:62	HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration.
28372195	0	52	dep	HMF	0:2	arg1	analysis					82:89	a chemometric analysis	68:89	a chemometric analysis for identification of honey freshness and adulteration	68:144	HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration.
28372195	4	53	theme	spectrometric	676:688	arg1	techniques					690:699	the kinetic and spectrometric techniques	660:699	the kinetic and spectrometric techniques using the certified reference material	660:738	Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values.
28372195	1	54	theme	content	243:249	arg1	determination					182:194	the determination	178:194	the determination of diastase activity and hydroxymethylfurfural content in honeys	178:259	A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods.
27759412	0	0	theme	manual	65:70	arg1	fabrication					72:82	manual fabrication	65:82	manual fabrication	65:82	Marginal fit of indirect composite inlays using a new system for manual fabrication.
27759412	5	1	theme	marginal	789:796	arg1	106.2μm					814:820	174.9μm ± 106.2μm	804:820	174.9μm ± 106.2μm	804:820	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	5	1	theme	marginal	789:796	arg1	gaps					798:801	the biggest marginal gaps	777:801	group A the biggest marginal gaps (174.9μm ± 106.2μm)	769:821	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	2	2	theme	silicone	545:552	arg1	D					566:566	D	566:566	D	566:566	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	2	theme	silicone	545:552	arg1	impression					554:563	silicone impression	545:563	silicone impression (D)	545:567	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	3	theme	study	345:349	arg1	design					351:356	study design	345:356	study design	345:356	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	9	4	from	production	1353:1362	arg1	laboratory					1413:1422	the dental laboratory	1402:1422	the dental laboratory	1402:1422	Nevertheless, production of indirect composite restorations in the dental laboratory showed the highest precision.
27759412	7	5	theme	significant	995:1005	arg1	differences					1007:1017	no significant differences	992:1017	no significant differences	992:1017	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	6	theme	±	956:956	arg1	68.0μm					958:963	64.6 μm ± 68.0μm	948:963	64.6 μm ± 68.0μm	948:963	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	6	theme	±	956:956	arg1	C					945:945	C	945:945	C (64.6 μm ± 68.0μm)	945:964	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	1	7	theme	manual	131:136	arg1	fabrication					149:159	manual chair side fabrication	131:159	manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques	131:316	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	1	8	theme	alginate	230:237	arg1	impressions					239:249	alginate impressions	230:249	alginate impressions	230:249	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	6	9	theme	±	892:892	arg1	μm					899:900	119.5 μm ± 90.6 μm	883:900	119.5 μm ± 90.6 μm	883:900	In group B the gaps were significantly smaller (119.5 μm ± 90.6 μm) than in group A (p=0.035).
27759412	9	10	theme	dental	1406:1411	arg1	laboratory					1413:1422	the dental laboratory	1402:1422	the dental laboratory	1402:1422	Nevertheless, production of indirect composite restorations in the dental laboratory showed the highest precision.
27759412	4	11	theme	Statistical	678:688	arg1	analysis					690:697	Statistical analysis	678:697	Statistical analysis	678:697	Statistical analysis was performed using ANOVA and Tukey's test.
27759412	9	12	theme	restorations	1386:1397	arg1	production					1353:1362	production	1353:1362	production of indirect composite restorations in the dental laboratory	1353:1422	Nevertheless, production of indirect composite restorations in the dental laboratory showed the highest precision.
27759412	3	13	theme	cement	642:647	arg1	gaps					649:652	the cement gaps	638:652	the cement gaps	638:652	The inlays were adapted into a metal tooth and silicone replicas of the cement gaps were made and measured.
27759412	5	14	theme	group	769:773	arg1	A					775:775	group A the biggest marginal gaps (174.9μm ± 106.2μm)	769:821	group A the biggest marginal gaps (174.9μm ± 106.2μm)	769:821	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	3	15	theme	gaps	649:652	arg1	replicas					626:633	silicone replicas	617:633	silicone replicas of the cement gaps	617:652	The inlays were adapted into a metal tooth and silicone replicas of the cement gaps were made and measured.
27759412	2	16	theme	different	405:413	arg1	system					486:491	the new inlay system	472:491	the new inlay system (B)	472:495	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	16	theme	different	405:413	arg1	model					506:510	plaster model	498:510	plaster model	498:510	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	16	theme	different	405:413	arg1	types					415:419	different types	405:419	different types of production techniques: CAD/CAM- technology (A)	405:469	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	1	17	theme	chair	138:142	arg1	fabrication					149:159	manual chair side fabrication	131:159	manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques	131:316	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	2	18	theme	inlay	480:484	arg1	system					486:491	the new inlay system	472:491	the new inlay system (B)	472:495	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	18	theme	inlay	480:484	arg1	types					415:419	different types	405:419	different types of production techniques: CAD/CAM- technology (A)	405:469	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	18	theme	inlay	480:484	arg1	B					494:494	B	494:494	B	494:494	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	5	19	theme	174.9μm	804:810	arg1	106.2μm					814:820	174.9μm ± 106.2μm	804:820	174.9μm ± 106.2μm	804:820	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	5	19	theme	174.9μm	804:810	arg1	gaps					798:801	the biggest marginal gaps	777:801	group A the biggest marginal gaps (174.9μm ± 106.2μm)	769:821	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	8	20	from	alternative	1326:1336	arg1	comparison					1229:1238	comparison	1229:1238	comparison to build restorations in a laboratory	1229:1276	In comparison to build restorations in a laboratory, the new system is a timesaving and inexpensive alternative.
27759412	2	21	theme	techniques	435:444	arg1	system					486:491	the new inlay system	472:491	the new inlay system (B)	472:495	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	21	theme	techniques	435:444	arg1	model					506:510	plaster model	498:510	plaster model	498:510	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	21	theme	techniques	435:444	arg1	types					415:419	different types	405:419	different types of production techniques: CAD/CAM- technology (A)	405:469	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	1	22	theme	side	144:147	arg1	fabrication					149:159	manual chair side fabrication	131:159	manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques	131:316	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	7	23	theme	μm	978:979	arg1	D					970:970	D	970:970	D (58.2 μm ± 61.7 μm)	970:990	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	23	theme	μm	978:979	arg1	μm					988:989	58.2 μm ± 61.7 μm	973:989	58.2 μm ± 61.7 μm	973:989	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	8	24	theme	inexpensive	1314:1324	arg1	system					1287:1292	the new system	1279:1292	the new system	1279:1292	In comparison to build restorations in a laboratory, the new system is a timesaving and inexpensive alternative.
27759412	8	24	theme	inexpensive	1314:1324	arg1	alternative					1326:1336	a timesaving and inexpensive alternative	1297:1336	a timesaving and inexpensive alternative	1297:1336	In comparison to build restorations in a laboratory, the new system is a timesaving and inexpensive alternative.
27759412	7	25	dep	found	1028:1032	arg1	p=0.998					1035:1041	p=0.998	1035:1041	p=0.998	1035:1041	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	8	26	theme	timesaving	1299:1308	arg1	system					1287:1292	the new system	1279:1292	the new system	1279:1292	In comparison to build restorations in a laboratory, the new system is a timesaving and inexpensive alternative.
27759412	8	26	theme	timesaving	1299:1308	arg1	alternative					1326:1336	a timesaving and inexpensive alternative	1297:1336	a timesaving and inexpensive alternative	1297:1336	In comparison to build restorations in a laboratory, the new system is a timesaving and inexpensive alternative.
27759412	2	27	theme	composite	366:374	arg1	inlays					376:381	Each 10 composite inlays	358:381	Each 10 composite inlays	358:381	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	0	28	theme	Marginal	0:7	arg1	fit					9:11	Marginal fit	0:11	Marginal fit of indirect composite inlays using a new system for manual fabrication.	0:83	Marginal fit of indirect composite inlays using a new system for manual fabrication.
27759412	2	29	dep	techniques	435:444	arg1	technology					456:465	CAD/CAM- technology	447:465	production techniques: CAD/CAM- technology (A)	424:469	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	29	dep	techniques	435:444	arg1	A					468:468	A	468:468	A	468:468	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	30	dep	MATRIALS	319:326	arg1	inlays					376:381	Each 10 composite inlays	358:381	Each 10 composite inlays	358:381	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	8	31	from	comparison	1229:1238	arg1	laboratory					1267:1276	a laboratory	1265:1276	a laboratory	1265:1276	In comparison to build restorations in a laboratory, the new system is a timesaving and inexpensive alternative.
27759412	5	32	theme	biggest	781:787	arg1	106.2μm					814:820	174.9μm ± 106.2μm	804:820	174.9μm ± 106.2μm	804:820	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	5	32	theme	biggest	781:787	arg1	gaps					798:801	the biggest marginal gaps	777:801	group A the biggest marginal gaps (174.9μm ± 106.2μm)	769:821	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	0	33	theme	indirect	16:23	arg1	inlays					35:40	indirect composite inlays	16:40	indirect composite inlays using a new system for manual fabrication	16:82	Marginal fit of indirect composite inlays using a new system for manual fabrication.
27759412	1	34	theme	CAD/CAM-technology	255:272	arg1	techniques					307:316	CAD/CAM-technology and laboratory manual production techniques	255:316	CAD/CAM-technology and laboratory manual production techniques	255:316	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	7	35	theme	μm	953:954	arg1	68.0μm					958:963	64.6 μm ± 68.0μm	948:963	64.6 μm ± 68.0μm	948:963	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	35	theme	μm	953:954	arg1	C					945:945	C	945:945	C (64.6 μm ± 68.0μm)	945:964	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	36	dep	Conclusion	1108:1117	arg1	resulted					1163:1170	resulted	1163:1170	resulted in better marginal precision than CAD/CAM technology	1163:1223	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	37	theme	marginal	1182:1189	arg1	precision					1191:1199	better marginal precision	1175:1199	better marginal precision than CAD/CAM technology	1175:1223	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	9	38	from	laboratory	1413:1422	arg1	production					1353:1362	production	1353:1362	production of indirect composite restorations in the dental laboratory	1353:1422	Nevertheless, production of indirect composite restorations in the dental laboratory showed the highest precision.
27759412	1	39	theme	indirect	164:171	arg1	restorations					183:194	indirect composite restorations	164:194	indirect composite restorations	164:194	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	7	40	theme	CAD/CAM	1206:1212	arg1	technology					1214:1223	CAD/CAM technology	1206:1223	CAD/CAM technology	1206:1223	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	2	41	theme	plaster	498:504	arg1	model					506:510	plaster model	498:510	plaster model	498:510	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	41	theme	plaster	498:504	arg1	types					415:419	different types	405:419	different types of production techniques: CAD/CAM- technology (A)	405:469	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	8	42	theme	build	1243:1247	arg1	restorations					1249:1260	build restorations	1243:1260	build restorations	1243:1260	In comparison to build restorations in a laboratory, the new system is a timesaving and inexpensive alternative.
27759412	1	43	theme	in	90:91	arg1	study					99:103	This in vitro study	85:103	This in vitro study	85:103	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	9	44	theme	highest	1435:1441	arg1	precision					1443:1451	the highest precision	1431:1451	the highest precision	1431:1451	Nevertheless, production of indirect composite restorations in the dental laboratory showed the highest precision.
27759412	1	45	theme	composite	173:181	arg1	restorations					183:194	indirect composite restorations	164:194	indirect composite restorations	164:194	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	0	46	theme	inlays	35:40	arg1	fit					9:11	Marginal fit	0:11	Marginal fit of indirect composite inlays using a new system for manual fabrication.	0:83	Marginal fit of indirect composite inlays using a new system for manual fabrication.
27759412	1	47	theme	laboratory	278:287	arg1	techniques					307:316	CAD/CAM-technology and laboratory manual production techniques	255:316	CAD/CAM-technology and laboratory manual production techniques	255:316	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	7	48	theme	±	981:981	arg1	D					970:970	D	970:970	D (58.2 μm ± 61.7 μm)	970:990	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	48	theme	±	981:981	arg1	μm					988:989	58.2 μm ± 61.7 μm	973:989	58.2 μm ± 61.7 μm	973:989	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	49	theme	group	1099:1103	arg1	Conclusion					1108:1117	group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology	1099:1223	group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology	1099:1223	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	1	50	theme	restorations	183:194	arg1	fabrication					149:159	manual chair side fabrication	131:159	manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques	131:316	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	0	51	theme	composite	25:33	arg1	inlays					35:40	indirect composite inlays	16:40	indirect composite inlays using a new system for manual fabrication	16:82	Marginal fit of indirect composite inlays using a new system for manual fabrication.
27759412	1	52	theme	manual	289:294	arg1	techniques					307:316	CAD/CAM-technology and laboratory manual production techniques	255:316	CAD/CAM-technology and laboratory manual production techniques	255:316	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	7	53	theme	B.	1105:1106	arg1	Conclusion					1108:1117	group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology	1099:1223	group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology	1099:1223	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	2	54	theme	production	424:433	arg1	techniques					435:444	production techniques	424:444	production techniques: CAD/CAM- technology (A)	424:469	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	5	55	from	Statistics	755:764	arg1	A					775:775	group A the biggest marginal gaps (174.9μm ± 106.2μm)	769:821	group A the biggest marginal gaps (174.9μm ± 106.2μm)	769:821	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	7	56	dep	groups	938:943	arg1	D					970:970	D	970:970	D (58.2 μm ± 61.7 μm)	970:990	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	56	dep	groups	938:943	arg1	groups					938:943	groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm)	938:990	groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm)	938:990	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	56	dep	groups	938:943	arg1	68.0μm					958:963	64.6 μm ± 68.0μm	948:963	64.6 μm ± 68.0μm	948:963	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	56	dep	groups	938:943	arg1	C					945:945	C	945:945	C (64.6 μm ± 68.0μm)	945:964	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	7	56	dep	groups	938:943	arg1	μm					988:989	58.2 μm ± 61.7 μm	973:989	58.2 μm ± 61.7 μm	973:989	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	1	57	theme	production	296:305	arg1	techniques					307:316	CAD/CAM-technology and laboratory manual production techniques	255:316	CAD/CAM-technology and laboratory manual production techniques	255:316	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	7	58	theme	inlays	1156:1161	arg1	manufacturing					1129:1141	Chairside manufacturing	1119:1141	Chairside manufacturing of composite inlays	1119:1161	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	5	59	theme	±	812:812	arg1	106.2μm					814:820	174.9μm ± 106.2μm	804:820	174.9μm ± 106.2μm	804:820	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	5	59	theme	±	812:812	arg1	gaps					798:801	the biggest marginal gaps	777:801	group A the biggest marginal gaps (174.9μm ± 106.2μm)	769:821	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	2	60	theme	CAD/CAM-	447:454	arg1	technology					456:465	CAD/CAM- technology	447:465	production techniques: CAD/CAM- technology (A)	424:469	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	60	theme	CAD/CAM-	447:454	arg1	A					468:468	A	468:468	A	468:468	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	6	61	dep	smaller	874:880	arg1	μm					899:900	119.5 μm ± 90.6 μm	883:900	119.5 μm ± 90.6 μm	883:900	In group B the gaps were significantly smaller (119.5 μm ± 90.6 μm) than in group A (p=0.035).
27759412	5	62	dep	A	775:775	arg1	106.2μm					814:820	174.9μm ± 106.2μm	804:820	174.9μm ± 106.2μm	804:820	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	5	62	dep	A	775:775	arg1	gaps					798:801	the biggest marginal gaps	777:801	group A the biggest marginal gaps (174.9μm ± 106.2μm)	769:821	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	5	63	from	Results	743:749	arg1	A					775:775	group A the biggest marginal gaps (174.9μm ± 106.2μm)	769:821	group A the biggest marginal gaps (174.9μm ± 106.2μm)	769:821	Results and Statistics In group A the biggest marginal gaps (174.9μm ± 106.2μm) were found.
27759412	9	64	theme	indirect	1367:1374	arg1	restorations					1386:1397	indirect composite restorations	1367:1397	indirect composite restorations in the dental laboratory	1367:1422	Nevertheless, production of indirect composite restorations in the dental laboratory showed the highest precision.
27759412	6	65	theme	group	838:842	arg1	B					844:844	group B	838:844	group B	838:844	In group B the gaps were significantly smaller (119.5 μm ± 90.6 μm) than in group A (p=0.035).
27759412	2	66	theme	alginate	518:525	arg1	C					539:539	C	539:539	C	539:539	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	66	theme	alginate	518:525	arg1	impression					527:536	alginate impression	518:536	alginate impression (C)	518:540	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	3	67	theme	metal	601:605	arg1	tooth					607:611	a metal tooth	599:611	a metal tooth	599:611	The inlays were adapted into a metal tooth and silicone replicas of the cement gaps were made and measured.
27759412	7	68	theme	better	1175:1180	arg1	precision					1191:1199	better marginal precision	1175:1199	better marginal precision than CAD/CAM technology	1175:1223	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	9	69	theme	composite	1376:1384	arg1	restorations					1386:1397	indirect composite restorations	1367:1397	indirect composite restorations in the dental laboratory	1367:1422	Nevertheless, production of indirect composite restorations in the dental laboratory showed the highest precision.
27759412	7	70	theme	composite	1146:1154	arg1	inlays					1156:1161	composite inlays	1146:1161	composite inlays	1146:1161	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	8	71	theme	new	1283:1285	arg1	system					1287:1292	the new system	1279:1292	the new system	1279:1292	In comparison to build restorations in a laboratory, the new system is a timesaving and inexpensive alternative.
27759412	8	71	theme	new	1283:1285	arg1	alternative					1326:1336	a timesaving and inexpensive alternative	1297:1336	a timesaving and inexpensive alternative	1297:1336	In comparison to build restorations in a laboratory, the new system is a timesaving and inexpensive alternative.
27759412	2	72	theme	new	476:478	arg1	system					486:491	the new inlay system	472:491	the new inlay system (B)	472:495	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	72	theme	new	476:478	arg1	types					415:419	different types	405:419	different types of production techniques: CAD/CAM- technology (A)	405:469	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	2	72	theme	new	476:478	arg1	B					494:494	B	494:494	B	494:494	MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D).
27759412	7	73	theme	Chairside	1119:1127	arg1	manufacturing					1129:1141	Chairside manufacturing	1119:1141	Chairside manufacturing of composite inlays	1119:1161	Between groups C (64.6 μm ± 68.0μm) and D (58.2 μm ± 61.7 μm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology.
27759412	6	74	theme	group	911:915	arg1	p=0.035					920:926	p=0.035	920:926	p=0.035	920:926	In group B the gaps were significantly smaller (119.5 μm ± 90.6 μm) than in group A (p=0.035).
27759412	6	74	theme	group	911:915	arg1	A					917:917	group A	911:917	group A (p=0.035)	911:927	In group B the gaps were significantly smaller (119.5 μm ± 90.6 μm) than in group A (p=0.035).
27759412	1	75	theme	new	116:118	arg1	system					120:125	a new system	114:125	a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques	114:316	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	9	76	from	restorations	1386:1397	arg1	laboratory					1413:1422	the dental laboratory	1402:1422	the dental laboratory	1402:1422	Nevertheless, production of indirect composite restorations in the dental laboratory showed the highest precision.
27759412	1	77	theme	silicone	208:215	arg1	models					217:222	silicone models	208:222	silicone models	208:222	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	0	78	theme	new	50:52	arg1	system					54:59	a new system	48:59	a new system for manual fabrication	48:82	Marginal fit of indirect composite inlays using a new system for manual fabrication.
27759412	3	79	theme	silicone	617:624	arg1	replicas					626:633	silicone replicas	617:633	silicone replicas of the cement gaps	617:652	The inlays were adapted into a metal tooth and silicone replicas of the cement gaps were made and measured.
27759412	1	80	dep	in	90:91	arg1	vitro					93:97	vitro	93:97	vitro	93:97	This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques.
27759412	6	81	theme	μm	889:890	arg1	μm					899:900	119.5 μm ± 90.6 μm	883:900	119.5 μm ± 90.6 μm	883:900	In group B the gaps were significantly smaller (119.5 μm ± 90.6 μm) than in group A (p=0.035).
26891290	0	0	theme	Anti-Biofilm	87:98	arg1	Surfaces					100:107	Efficient Anti-Biofilm Surfaces	77:107	Efficient Anti-Biofilm Surfaces	77:107	Bioactive ZnO Coatings Deposited by MAPLE-An Appropriate Strategy to Produce Efficient Anti-Biofilm Surfaces.
26891290	1	1	theme	Laser	250:254	arg1	technique					276:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique.
26891290	0	2	theme	Efficient	77:85	arg1	Surfaces					100:107	Efficient Anti-Biofilm Surfaces	77:107	Efficient Anti-Biofilm Surfaces	77:107	Bioactive ZnO Coatings Deposited by MAPLE-An Appropriate Strategy to Produce Efficient Anti-Biofilm Surfaces.
26891290	2	3	theme	IR	356:357	arg1	microscopy					359:368	IR microscopy	356:368	IR microscopy	356:368	The obtained nanostructures were characterized by X-ray diffraction, IR microscopy and scanning electron microscopy.
26891290	1	4	theme	Evaporation	256:266	arg1	technique					276:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique.
26891290	5	5	theme	cephalosporin	768:780	arg1	Cfp					782:784	the fourth generation cephalosporin Cfp	746:784	the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix	746:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	4	6	theme	in	532:533	arg1	tests					540:544	In vitro and in vivo tests	519:544	In vitro and in vivo tests	519:544	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	5	7	theme	infections	1006:1015	arg1	risk					982:985	the risk	978:985	the risk of biofilm-related infections	978:1015	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	5	8	theme	Cfp	782:784	arg1	release					735:741	The release	731:741	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix	731:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	1	9	theme	MAPLE	269:273	arg1	technique					276:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique.
26891290	5	10	theme	biofilm	917:923	arg1	development					925:935	the subsequent colonization and biofilm development	885:935	development	925:935	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	4	11	theme	surfaces	692:699	arg1	development					663:673	the development	659:673	the development of antimicrobial surfaces with biomedical applications	659:728	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	4	12	theme	In	519:520	arg1	tests					540:544	In vitro and in vivo tests	519:544	In vitro and in vivo tests	519:544	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	5	13	from	release	735:741	arg1	form					811:814	a biologically active form	789:814	a biologically active form from the ZnO matrix	789:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	4	14	theme	antimicrobial	678:690	arg1	surfaces					692:699	antimicrobial surfaces	678:699	antimicrobial surfaces with biomedical applications	678:728	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	0	15	theme	ZnO	10:12	arg1	Coatings					14:21	Bioactive ZnO Coatings	0:21	Bioactive ZnO Coatings	0:21	Bioactive ZnO Coatings Deposited by MAPLE-An Appropriate Strategy to Produce Efficient Anti-Biofilm Surfaces.
26891290	4	16	theme	biomedical	706:715	arg1	applications					717:728	biomedical applications	706:728	biomedical applications	706:728	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	0	17	theme	Bioactive	0:8	arg1	Coatings					14:21	Bioactive ZnO Coatings	0:21	Bioactive ZnO Coatings	0:21	Bioactive ZnO Coatings Deposited by MAPLE-An Appropriate Strategy to Produce Efficient Anti-Biofilm Surfaces.
26891290	4	18	dep	In	519:520	arg1	vitro					522:526	vitro	522:526	vitro	522:526	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	2	19	theme	electron	383:390	arg1	microscopy					392:401	scanning electron microscopy	374:401	scanning electron microscopy	374:401	The obtained nanostructures were characterized by X-ray diffraction, IR microscopy and scanning electron microscopy.
26891290	4	20	theme	good	562:565	arg1	biocompatibility					567:582	a good biocompatibility	560:582	a good biocompatibility	560:582	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	5	21	theme	biofilm-related	990:1004	arg1	infections					1006:1015	biofilm-related infections	990:1015	biofilm-related infections	990:1015	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	5	22	theme	bacterial	862:870	arg1	adhesion					872:879	the bacterial adhesion	858:879	the bacterial adhesion	858:879	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	4	23	theme	ZnO/CD/Cfp	587:596	arg1	coatings					598:605	ZnO/CD/Cfp coatings	587:605	ZnO/CD/Cfp coatings	587:605	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	2	24	theme	obtained	291:298	arg1	nanostructures					300:313	The obtained nanostructures	287:313	The obtained nanostructures	287:313	The obtained nanostructures were characterized by X-ray diffraction, IR microscopy and scanning electron microscopy.
26891290	1	25	theme	zinc	155:158	arg1	oxide					160:164	zinc oxide	155:164	zinc oxide	155:164	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique.
26891290	1	25	theme	zinc	155:158	arg1	cefepime					184:191	cefepime	184:191	cefepime	184:191	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique.
26891290	1	25	theme	zinc	155:158	arg1	cyclodextrin					167:178	cyclodextrin	167:178	cyclodextrin	167:178	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique.
26891290	0	26	theme	MAPLE-An	36:43	arg1	Strategy					57:64	MAPLE-An Appropriate Strategy	36:64	MAPLE-An Appropriate Strategy to Produce Efficient Anti-Biofilm Surfaces	36:107	Bioactive ZnO Coatings Deposited by MAPLE-An Appropriate Strategy to Produce Efficient Anti-Biofilm Surfaces.
26891290	5	27	theme	fourth	750:755	arg1	Cfp					782:784	the fourth generation cephalosporin Cfp	746:784	the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix	746:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	5	28	theme	generation	757:766	arg1	Cfp					782:784	the fourth generation cephalosporin Cfp	746:784	the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix	746:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	3	29	theme	increased	461:469	arg1	activity					484:491	an increased anti-biofilm activity	458:491	an increased anti-biofilm activity of ZnO/CD/Cfp composites	458:516	The efficient release of cefepime was correlated with an increased anti-biofilm activity of ZnO/CD/Cfp composites.
26891290	3	30	theme	cefepime	429:436	arg1	release					418:424	The efficient release	404:424	The efficient release of cefepime	404:436	The efficient release of cefepime was correlated with an increased anti-biofilm activity of ZnO/CD/Cfp composites.
26891290	4	31	theme	coatings	598:605	arg1	biocompatibility					567:582	a good biocompatibility	560:582	a good biocompatibility	560:582	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	4	32	with	surfaces	692:699	arg1	applications					717:728	biomedical applications	706:728	biomedical applications	706:728	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	3	33	theme	anti-biofilm	471:482	arg1	activity					484:491	an increased anti-biofilm activity	458:491	an increased anti-biofilm activity of ZnO/CD/Cfp composites	458:516	The efficient release of cefepime was correlated with an increased anti-biofilm activity of ZnO/CD/Cfp composites.
26891290	3	34	theme	efficient	408:416	arg1	release					418:424	The efficient release	404:424	The efficient release of cefepime	404:436	The efficient release of cefepime was correlated with an increased anti-biofilm activity of ZnO/CD/Cfp composites.
26891290	4	35	dep	in	532:533	arg1	vivo					535:538	vivo	535:538	vivo	535:538	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	5	36	from	Cfp	782:784	arg1	matrix					829:834	the ZnO matrix	821:834	the ZnO matrix	821:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	5	36	from	Cfp	782:784	arg1	form					811:814	a biologically active form	789:814	a biologically active form from the ZnO matrix	789:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	1	37	theme	bioactive	124:132	arg1	coatings					134:141	bioactive coatings	124:141	bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp)	124:204	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique.
26891290	0	38	theme	Appropriate	45:55	arg1	Strategy					57:64	MAPLE-An Appropriate Strategy	36:64	MAPLE-An Appropriate Strategy to Produce Efficient Anti-Biofilm Surfaces	36:107	Bioactive ZnO Coatings Deposited by MAPLE-An Appropriate Strategy to Produce Efficient Anti-Biofilm Surfaces.
26891290	3	39	theme	ZnO/CD/Cfp	496:505	arg1	composites					507:516	ZnO/CD/Cfp composites	496:516	ZnO/CD/Cfp composites	496:516	The efficient release of cefepime was correlated with an increased anti-biofilm activity of ZnO/CD/Cfp composites.
26891290	5	40	from	form	811:814	arg1	release					735:741	The release	731:741	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix	731:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	1	41	theme	coatings	134:141	arg1	Deposition					110:119	Deposition	110:119	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp)	110:204	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique.
26891290	5	42	theme	ZnO	825:827	arg1	matrix					829:834	the ZnO matrix	821:834	the ZnO matrix	821:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	2	43	theme	X-ray	337:341	arg1	diffraction					343:353	X-ray diffraction	337:353	X-ray diffraction	337:353	The obtained nanostructures were characterized by X-ray diffraction, IR microscopy and scanning electron microscopy.
26891290	3	44	theme	composites	507:516	arg1	activity					484:491	an increased anti-biofilm activity	458:491	an increased anti-biofilm activity of ZnO/CD/Cfp composites	458:516	The efficient release of cefepime was correlated with an increased anti-biofilm activity of ZnO/CD/Cfp composites.
26891290	1	45	theme	Matrix	227:232	arg1	technique					276:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique.
26891290	5	46	theme	subsequent	889:898	arg1	colonization					900:911	the subsequent colonization and biofilm development	885:935	colonization	900:911	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	2	47	theme	scanning	374:381	arg1	microscopy					392:401	scanning electron microscopy	374:401	scanning electron microscopy	374:401	The obtained nanostructures were characterized by X-ray diffraction, IR microscopy and scanning electron microscopy.
26891290	1	48	theme	Assisted	234:241	arg1	technique					276:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique.
26891290	5	49	theme	active	804:809	arg1	form					811:814	a biologically active form	789:814	a biologically active form from the ZnO matrix	789:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	5	50	from	matrix	829:834	arg1	form					811:814	a biologically active form	789:814	a biologically active form from the ZnO matrix	789:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	5	50	from	matrix	829:834	arg1	Cfp					782:784	the fourth generation cephalosporin Cfp	746:784	the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix	746:834	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	4	51	theme	competitive	632:642	arg1	them					624:627	them	624:627	them	624:627	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	4	51	theme	competitive	632:642	arg1	candidates					644:653	competitive candidates	632:653	competitive candidates for the development of antimicrobial surfaces with biomedical applications	632:728	In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications.
26891290	5	52	theme	various	940:946	arg1	surfaces					948:955	various surfaces	940:955	various surfaces	940:955	The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
26891290	1	53	theme	Pulsed	243:248	arg1	technique					276:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique	223:284	Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique.
24053536	0	0	theme	tissue	100:105	arg1	engineering					107:117	bone tissue engineering	95:117	bone tissue engineering	95:117	Preparation and characterization of bionic bone structure chitosan/hydroxyapatite scaffold for bone tissue engineering.
24053536	7	1	theme	CS	1303:1304	arg1	scaffolds					1306:1314	the pure CS scaffolds	1294:1314	the pure CS scaffolds	1294:1314	The ALP activity value of composite scaffolds was at least six times of the pure CS scaffolds.
24053536	5	2	theme	MC3T3-E1	925:932	arg1	cell					934:937	MC3T3-E1 cell	925:937	MC3T3-E1 cell	925:937	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	9	3	theme	compressive	1405:1415	arg1	strength					1417:1424	The compressive strength	1401:1424	The compressive strength of CS/HA15	1401:1435	The compressive strength of CS/HA15 increased by 33.07% compared with the pure CS scaffold.
24053536	0	4	theme	bone	95:98	arg1	engineering					107:117	bone tissue engineering	95:117	bone tissue engineering	95:117	Preparation and characterization of bionic bone structure chitosan/hydroxyapatite scaffold for bone tissue engineering.
24053536	2	5	contain	had	349:351	arg2	multilayer					369:378	multilayer	369:378	multilayer	369:378	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	2	5	contain	had	349:351	arg1	scaffolds					317:325	the scaffolds	313:325	the scaffolds with acicular nano-HA	313:347	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	2	5	contain	had	349:351	arg2	structure					391:399	porous structure	384:399	porous structure	384:399	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	2	6	theme	spoke-like	357:366	arg1	multilayer					369:378	multilayer	369:378	multilayer	369:378	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	9	7	theme	CS	1480:1481	arg1	scaffold					1483:1490	the pure CS scaffold	1471:1490	the pure CS scaffold	1471:1490	The compressive strength of CS/HA15 increased by 33.07% compared with the pure CS scaffold.
24053536	7	8	theme	scaffolds	1306:1314	arg1	times					1285:1289	at least six times	1272:1289	at least six times of the pure CS scaffolds	1272:1314	The ALP activity value of composite scaffolds was at least six times of the pure CS scaffolds.
24053536	7	8	theme	scaffolds	1306:1314	arg1	value					1239:1243	The ALP activity value	1222:1243	The ALP activity value of composite scaffolds	1222:1266	The ALP activity value of composite scaffolds was at least six times of the pure CS scaffolds.
24053536	5	9	theme	CS/HA10	983:989	arg1	scaffold					991:998	the CS/HA10 scaffold	979:998	the CS/HA10 scaffold	979:998	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	5	9	theme	CS/HA10	983:989	arg1	one					1016:1018	one	1016:1018	one	1016:1018	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	6	10	theme	CS	1152:1153	arg1	scaffold					1155:1162	the pure CS scaffold	1143:1162	the pure CS scaffold	1143:1162	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	4	11	theme	X-ray	589:593	arg1	diffractometer					602:615	X-ray powder diffractometer	589:615	X-ray powder diffractometer	589:615	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	5	12	theme	CS	962:963	arg1	scaffolds					965:973	the pure CS scaffolds	953:973	the pure CS scaffolds	953:973	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	6	13	theme	value	1108:1112	arg1	ratio					1058:1062	a higher ratio	1049:1062	a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts	1049:1127	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	2	14	with	scaffolds	317:325	arg1	nano-HA					341:347	acicular nano-HA	332:347	acicular nano-HA	332:347	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	3	15	theme	cell	507:510	arg1	culture					512:518	seven-day cell culture	497:518	seven-day cell culture	497:518	The SEM of osteoblasts which were polygonal or spindle-shaped on the composite scaffolds after seven-day cell culture showed that the cells grew, adhered, and spread well.
24053536	5	16	theme	composite	862:870	arg1	scaffolds					872:880	the composite scaffolds	858:880	the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds	858:973	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	9	17	theme	CS/HA15	1429:1435	arg1	strength					1417:1424	The compressive strength	1401:1424	The compressive strength of CS/HA15	1401:1435	The compressive strength of CS/HA15 increased by 33.07% compared with the pure CS scaffold.
24053536	6	18	theme	phosphate	1089:1097	arg1	activity					1099:1106	alkaline phosphate activity	1080:1106	alkaline phosphate activity	1080:1106	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	5	19	theme	In	811:812	arg1	evaluation					837:846	In vitro biocompatibility evaluation	811:846	In vitro biocompatibility evaluation	811:846	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	4	20	contain	had	721:723	arg2	structure					731:739	phase structure	725:739	phase structure similar to natural bone	725:763	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	4	20	contain	had	721:723	arg1	particles					685:693	the mineral particles	673:693	the mineral particles deposited in the scaffold	673:719	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	3	21	theme	osteoblasts	413:423	arg1	SEM					406:408	The SEM	402:408	The SEM of osteoblasts which were polygonal or spindle-shaped on the composite scaffolds after seven-day cell culture	402:518	The SEM of osteoblasts which were polygonal or spindle-shaped on the composite scaffolds after seven-day cell culture showed that the cells grew, adhered, and spread well.
24053536	4	22	theme	infrared	639:646	arg1	spectrometer					648:659	infrared spectrometer	639:659	infrared spectrometer	639:659	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	6	23	contain	had	1045:1047	arg1	scaffold					1031:1038	The CS/HA scaffold	1021:1038	The CS/HA scaffold	1021:1038	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	6	23	contain	had	1045:1047	arg2	ratio					1058:1062	a higher ratio	1049:1062	a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts	1049:1127	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	6	24	theme	activity	1099:1106	arg1	value					1108:1112	adhesion and alkaline phosphate activity value	1067:1112	adhesion and alkaline phosphate activity value of osteoblasts	1067:1127	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	5	25	theme	higher	891:896	arg1	degree					898:903	a higher degree	889:903	a higher degree of proliferation of MC3T3-E1 cell	889:937	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	5	26	theme	cell	934:937	arg1	proliferation					908:920	proliferation	908:920	proliferation of MC3T3-E1 cell	908:937	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	10	27	theme	tissue	1605:1610	arg1	engineering					1612:1622	bone tissue engineering	1600:1622	bone tissue engineering	1600:1622	This novel porous scaffold with three-dimensional oriented structure might have a potential application in bone tissue engineering.
24053536	4	28	theme	natural	752:758	arg1	bone					760:763	natural bone	752:763	natural bone	752:763	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	7	29	theme	pure	1298:1301	arg1	scaffolds					1306:1314	the pure CS scaffolds	1294:1314	the pure CS scaffolds	1294:1314	The ALP activity value of composite scaffolds was at least six times of the pure CS scaffolds.
24053536	5	30	theme	biocompatibility	820:835	arg1	evaluation					837:846	In vitro biocompatibility evaluation	811:846	In vitro biocompatibility evaluation	811:846	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	10	31	theme	oriented	1543:1550	arg1	structure					1552:1560	three-dimensional oriented structure	1525:1560	three-dimensional oriented structure	1525:1560	This novel porous scaffold with three-dimensional oriented structure might have a potential application in bone tissue engineering.
24053536	2	32	theme	porous	384:389	arg1	structure					391:399	porous structure	384:399	porous structure	384:399	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	10	33	theme	bone	1600:1603	arg1	engineering					1612:1622	bone tissue engineering	1600:1622	bone tissue engineering	1600:1622	This novel porous scaffold with three-dimensional oriented structure might have a potential application in bone tissue engineering.
24053536	1	34	dep	in	209:210	arg1	situ					212:215	situ	212:215	situ	212:215	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds were prepared via in situ precipitation method in this research.
24053536	5	35	theme	pure	957:960	arg1	scaffolds					965:973	the pure CS scaffolds	953:973	the pure CS scaffolds	953:973	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	1	36	theme	Three-dimensional	120:136	arg1	HA					177:178	HA	177:178	HA	177:178	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds were prepared via in situ precipitation method in this research.
24053536	1	36	theme	Three-dimensional	120:136	arg1	/hydroxyapatite					160:174	Three-dimensional oriented chitosan (CS)/hydroxyapatite	120:174	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds	120:189	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds were prepared via in situ precipitation method in this research.
24053536	6	37	theme	alkaline	1080:1087	arg1	activity					1099:1106	alkaline phosphate activity	1080:1106	alkaline phosphate activity	1080:1106	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	1	38	theme	in	209:210	arg1	method					231:236	in situ precipitation method	209:236	in situ precipitation method in this research	209:253	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds were prepared via in situ precipitation method in this research.
24053536	8	39	contain	possessed	1368:1376	arg1	scaffolds					1358:1366	the composite scaffolds	1344:1366	the composite scaffolds	1344:1366	The results suggested that the composite scaffolds possessed good biocompatibility.
24053536	8	39	contain	possessed	1368:1376	arg2	biocompatibility					1383:1398	good biocompatibility	1378:1398	good biocompatibility	1378:1398	The results suggested that the composite scaffolds possessed good biocompatibility.
24053536	1	40	theme	oriented	138:145	arg1	HA					177:178	HA	177:178	HA	177:178	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds were prepared via in situ precipitation method in this research.
24053536	1	40	theme	oriented	138:145	arg1	/hydroxyapatite					160:174	Three-dimensional oriented chitosan (CS)/hydroxyapatite	120:174	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds	120:189	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds were prepared via in situ precipitation method in this research.
24053536	5	41	theme	proliferation	908:920	arg1	degree					898:903	a higher degree	889:903	a higher degree of proliferation of MC3T3-E1 cell	889:937	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	0	42	theme	bone	43:46	arg1	structure					48:56	bionic bone structure	36:56	bionic bone structure	36:56	Preparation and characterization of bionic bone structure chitosan/hydroxyapatite scaffold for bone tissue engineering.
24053536	10	43	contain	have	1568:1571	arg2	application					1585:1595	a potential application	1573:1595	a potential application	1573:1595	This novel porous scaffold with three-dimensional oriented structure might have a potential application in bone tissue engineering.
24053536	10	43	contain	have	1568:1571	arg1	scaffold					1511:1518	This novel porous scaffold	1493:1518	This novel porous scaffold with three-dimensional oriented structure	1493:1560	This novel porous scaffold with three-dimensional oriented structure might have a potential application in bone tissue engineering.
24053536	6	44	theme	higher	1051:1056	arg1	ratio					1058:1062	a higher ratio	1049:1062	a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts	1049:1127	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	4	45	theme	mineral	677:683	arg1	particles					685:693	the mineral particles	673:693	the mineral particles deposited in the scaffold	673:719	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	1	46	theme	precipitation	217:229	arg1	method					231:236	in situ precipitation method	209:236	in situ precipitation method in this research	209:253	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds were prepared via in situ precipitation method in this research.
24053536	0	47	theme	bionic	36:41	arg1	structure					48:56	bionic bone structure	36:56	bionic bone structure	36:56	Preparation and characterization of bionic bone structure chitosan/hydroxyapatite scaffold for bone tissue engineering.
24053536	10	48	theme	potential	1575:1583	arg1	application					1585:1595	a potential application	1573:1595	a potential application	1573:1595	This novel porous scaffold with three-dimensional oriented structure might have a potential application in bone tissue engineering.
24053536	10	49	theme	three-dimensional	1525:1541	arg1	structure					1552:1560	three-dimensional oriented structure	1525:1560	three-dimensional oriented structure	1525:1560	This novel porous scaffold with three-dimensional oriented structure might have a potential application in bone tissue engineering.
24053536	8	50	theme	good	1378:1381	arg1	biocompatibility					1383:1398	good biocompatibility	1378:1398	good biocompatibility	1378:1398	The results suggested that the composite scaffolds possessed good biocompatibility.
24053536	9	51	theme	pure	1475:1478	arg1	scaffold					1483:1490	the pure CS scaffold	1471:1490	the pure CS scaffold	1471:1490	The compressive strength of CS/HA15 increased by 33.07% compared with the pure CS scaffold.
24053536	10	52	theme	porous	1504:1509	arg1	scaffold					1511:1518	This novel porous scaffold	1493:1518	This novel porous scaffold with three-dimensional oriented structure	1493:1560	This novel porous scaffold with three-dimensional oriented structure might have a potential application in bone tissue engineering.
24053536	8	53	theme	composite	1348:1356	arg1	scaffolds					1358:1366	the composite scaffolds	1344:1366	the composite scaffolds	1344:1366	The results suggested that the composite scaffolds possessed good biocompatibility.
24053536	7	54	theme	ALP	1226:1228	arg1	value					1239:1243	The ALP activity value	1222:1243	The ALP activity value of composite scaffolds	1222:1266	The ALP activity value of composite scaffolds was at least six times of the pure CS scaffolds.
24053536	7	54	theme	ALP	1226:1228	arg1	times					1285:1289	at least six times	1272:1289	at least six times of the pure CS scaffolds	1272:1314	The ALP activity value of composite scaffolds was at least six times of the pure CS scaffolds.
24053536	1	55	from	method	231:236	arg1	research					246:253	this research	241:253	this research	241:253	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds were prepared via in situ precipitation method in this research.
24053536	1	56	theme	chitosan	147:154	arg1	HA					177:178	HA	177:178	HA	177:178	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds were prepared via in situ precipitation method in this research.
24053536	1	56	theme	chitosan	147:154	arg1	/hydroxyapatite					160:174	Three-dimensional oriented chitosan (CS)/hydroxyapatite	120:174	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds	120:189	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds were prepared via in situ precipitation method in this research.
24053536	0	57	theme	structure	48:56	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of bionic bone structure chitosan/hydroxyapatite scaffold for bone tissue engineering.
24053536	0	57	theme	structure	48:56	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of bionic bone structure chitosan/hydroxyapatite scaffold for bone tissue engineering.
24053536	6	58	theme	adhesion	1067:1074	arg1	value					1108:1112	adhesion and alkaline phosphate activity value	1067:1112	adhesion and alkaline phosphate activity value of osteoblasts	1067:1127	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	4	59	dep	Fourier	621:627	arg1	transform					629:637	transform	629:637	transform infrared spectrometer	629:659	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	5	60	dep	In	811:812	arg1	vitro					814:818	vitro	814:818	vitro	814:818	In vitro biocompatibility evaluation indicated the composite scaffolds showed a higher degree of proliferation of MC3T3-E1 cell compared with the pure CS scaffolds and the CS/HA10 scaffold was the highest one.
24053536	6	61	theme	content	1213:1219	arg1	increase					1198:1205	the increase	1194:1205	the increase of HA content	1194:1219	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	4	62	theme	phase	725:729	arg1	structure					731:739	phase structure	725:739	phase structure similar to natural bone	725:763	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	6	63	theme	HA	1210:1211	arg1	content					1213:1219	HA content	1210:1219	HA content	1210:1219	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	4	64	theme	similar	741:747	arg1	structure					731:739	phase structure	725:739	phase structure similar to natural bone	725:763	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	4	65	theme	diffractometer	602:615	arg1	results					578:584	The results	574:584	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer	574:659	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	10	66	theme	novel	1498:1502	arg1	scaffold					1511:1518	This novel porous scaffold	1493:1518	This novel porous scaffold with three-dimensional oriented structure	1493:1560	This novel porous scaffold with three-dimensional oriented structure might have a potential application in bone tissue engineering.
24053536	2	67	theme	microscopy	274:283	arg1	images					291:296	Scanning electron microscopy (SEM) images	256:296	Scanning electron microscopy (SEM) images	256:296	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	2	68	theme	acicular	332:339	arg1	nano-HA					341:347	acicular nano-HA	332:347	acicular nano-HA	332:347	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	6	69	theme	pure	1147:1150	arg1	scaffold					1155:1162	the pure CS scaffold	1143:1162	the pure CS scaffold	1143:1162	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	4	70	theme	powder	595:600	arg1	diffractometer					602:615	X-ray powder diffractometer	589:615	X-ray powder diffractometer	589:615	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	10	71	with	scaffold	1511:1518	arg1	structure					1552:1560	three-dimensional oriented structure	1525:1560	three-dimensional oriented structure	1525:1560	This novel porous scaffold with three-dimensional oriented structure might have a potential application in bone tissue engineering.
24053536	2	72	theme	electron	265:272	arg1	SEM					286:288	SEM	286:288	SEM	286:288	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	2	72	theme	electron	265:272	arg1	microscopy					274:283	Scanning electron microscopy	256:283	Scanning electron microscopy (SEM) images	256:296	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	3	73	theme	composite	471:479	arg1	scaffolds					481:489	the composite scaffolds	467:489	the composite scaffolds	467:489	The SEM of osteoblasts which were polygonal or spindle-shaped on the composite scaffolds after seven-day cell culture showed that the cells grew, adhered, and spread well.
24053536	1	74	theme	/hydroxyapatite	160:174	arg1	scaffolds					181:189	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds	120:189	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds	120:189	Three-dimensional oriented chitosan (CS)/hydroxyapatite (HA) scaffolds were prepared via in situ precipitation method in this research.
24053536	3	75	theme	seven-day	497:505	arg1	culture					512:518	seven-day cell culture	497:518	seven-day cell culture	497:518	The SEM of osteoblasts which were polygonal or spindle-shaped on the composite scaffolds after seven-day cell culture showed that the cells grew, adhered, and spread well.
24053536	4	76	theme	Fourier	621:627	arg1	results					578:584	The results	574:584	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer	574:659	The results of X-ray powder diffractometer and Fourier transform infrared spectrometer showed that the mineral particles deposited in the scaffold had phase structure similar to natural bone and confirmed that particles were exactly HA.
24053536	6	77	theme	osteoblasts	1117:1127	arg1	value					1108:1112	adhesion and alkaline phosphate activity value	1067:1112	adhesion and alkaline phosphate activity value of osteoblasts	1067:1127	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
24053536	2	78	theme	Scanning	256:263	arg1	SEM					286:288	SEM	286:288	SEM	286:288	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	2	78	theme	Scanning	256:263	arg1	microscopy					274:283	Scanning electron microscopy	256:283	Scanning electron microscopy (SEM) images	256:296	Scanning electron microscopy (SEM) images indicated that the scaffolds with acicular nano-HA had the spoke-like, multilayer and porous structure.
24053536	7	79	theme	scaffolds	1258:1266	arg1	value					1239:1243	The ALP activity value	1222:1243	The ALP activity value of composite scaffolds	1222:1266	The ALP activity value of composite scaffolds was at least six times of the pure CS scaffolds.
24053536	7	79	theme	scaffolds	1258:1266	arg1	times					1285:1289	at least six times	1272:1289	at least six times of the pure CS scaffolds	1272:1314	The ALP activity value of composite scaffolds was at least six times of the pure CS scaffolds.
24053536	7	80	theme	composite	1248:1256	arg1	scaffolds					1258:1266	composite scaffolds	1248:1266	composite scaffolds	1248:1266	The ALP activity value of composite scaffolds was at least six times of the pure CS scaffolds.
24053536	7	81	theme	activity	1230:1237	arg1	value					1239:1243	The ALP activity value	1222:1243	The ALP activity value of composite scaffolds	1222:1266	The ALP activity value of composite scaffolds was at least six times of the pure CS scaffolds.
24053536	7	81	theme	activity	1230:1237	arg1	times					1285:1289	at least six times	1272:1289	at least six times of the pure CS scaffolds	1272:1314	The ALP activity value of composite scaffolds was at least six times of the pure CS scaffolds.
24053536	6	82	theme	CS/HA	1025:1029	arg1	scaffold					1031:1038	The CS/HA scaffold	1021:1038	The CS/HA scaffold	1021:1038	The CS/HA scaffold also had a higher ratio of adhesion and alkaline phosphate activity value of osteoblasts compared with the pure CS scaffold, and the ratio increased with the increase of HA content.
26746104	6	0	theme	purification	946:957	arg1	procedures					959:968	several purification procedures	938:968	several purification procedures	938:968	Therefore, several purification procedures were tested to efficiently remove detergent, while retaining maximum protein recoveries.
26746104	9	1	theme	new	1493:1495	arg1	method					1469:1474	This method	1464:1474	This method	1464:1474	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	9	1	theme	new	1493:1495	arg1	tool					1508:1511	a new analytical tool	1491:1511	a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins	1491:1626	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	3	2	theme	reproducible	566:577	arg1	methods					590:596	efficient and reproducible analytical methods	552:596	efficient and reproducible analytical methods	552:596	The composition of N-glycans attached to membrane proteins has not been sufficiently studied due to the lack of efficient and reproducible analytical methods.
26746104	1	3	theme	Numerous	124:131	arg1	proteins					133:140	BACKGROUND Numerous proteins	113:140	BACKGROUND Numerous proteins depend on correct glycosylation for their proper function and nearly all membrane	113:222	BACKGROUND Numerous proteins depend on correct glycosylation for their proper function and nearly all membrane, as well as secreted, proteins are glycosylated.
26746104	7	4	theme	protein	1242:1248	arg1	groups					1250:1255	the aforementioned protein groups	1223:1255	the aforementioned protein groups	1223:1255	RESULTS CPE showed to be an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups.
26746104	9	5	theme	reliable	1517:1524	arg1	detection					1526:1534	detection	1526:1534	detection	1526:1534	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	11	6	from	Glycans	1906:1912	arg1	medicine					1930:1937	personalised medicine	1917:1937	personalised medicine" Guest Editor	1917:1951	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26746104	8	7	theme	N-glycan	1335:1342	arg1	analysis					1344:1351	N-glycan analysis	1335:1351	N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material	1335:1449	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	2	8	theme	crucial	316:322	arg1	role					324:327	a crucial role	314:327	a crucial role	314:327	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	8	9	theme	resulting	1262:1270	arg1	protocol					1272:1279	The resulting protocol	1258:1279	The resulting protocol	1258:1279	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	2	10	from	recognition	375:385	arg1	surface					431:437	the cell surface	422:437	the cell surface	422:437	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	3	11	theme	methods	590:596	arg1	lack					544:547	the lack	540:547	the lack of efficient and reproducible analytical methods	540:596	The composition of N-glycans attached to membrane proteins has not been sufficiently studied due to the lack of efficient and reproducible analytical methods.
26746104	8	12	theme	membrane	1361:1368	arg1	analysis					1344:1351	N-glycan analysis	1335:1351	N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material	1335:1449	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	5	13	theme	proteins	862:869	arg1	Purification					837:848	Purification	837:848	Purification of isolated proteins from the excess of detergent	837:898	Purification of isolated proteins from the excess of detergent proved to be the key step.
26746104	5	13	theme	proteins	862:869	arg1	step					921:924	the key step	913:924	the key step	913:924	Purification of isolated proteins from the excess of detergent proved to be the key step.
26746104	5	14	theme	detergent	890:898	arg1	excess					880:885	the excess	876:885	the excess of detergent	876:898	Purification of isolated proteins from the excess of detergent proved to be the key step.
26746104	8	15	theme	intracellular	1374:1386	arg1	proteins					1398:1405	both membrane and intracellular (soluble) proteins	1356:1405	proteins	1398:1405	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	10	16	theme	GENERAL	1703:1709	arg1	SIGNIFICANCE					1711:1722	GENERAL SIGNIFICANCE	1703:1722	GENERAL SIGNIFICANCE The simple method	1703:1740	GENERAL SIGNIFICANCE The simple method was successfully optimised to generate reliable HILIC-UPLC profiles of N-glycans released from membrane proteins.
26746104	11	17	theme	personalised	1917:1928	arg1	medicine					1930:1937	personalised medicine	1917:1937	personalised medicine" Guest Editor	1917:1951	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26746104	7	18	theme	efficient	1087:1095	arg1	CPE					1067:1069	RESULTS CPE	1059:1069	RESULTS CPE	1059:1069	RESULTS CPE showed to be an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups.
26746104	7	18	theme	efficient	1087:1095	arg1	method					1097:1102	an efficient method	1084:1102	an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups	1084:1255	RESULTS CPE showed to be an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups.
26746104	9	19	theme	type	1583:1586	arg1	N-glycans					1588:1596	oligomannose and complex type N-glycans	1558:1596	N-glycans	1588:1596	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	8	20	theme	soluble	1389:1395	arg1	proteins					1398:1405	both membrane and intracellular (soluble) proteins	1356:1405	proteins	1398:1405	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	5	21	from	excess	880:885	arg1	Purification					837:848	Purification	837:848	Purification of isolated proteins from the excess of detergent	837:898	Purification of isolated proteins from the excess of detergent proved to be the key step.
26746104	5	21	from	excess	880:885	arg1	proteins					862:869	isolated proteins	853:869	isolated proteins from the excess of detergent	853:898	Purification of isolated proteins from the excess of detergent proved to be the key step.
26746104	5	21	from	excess	880:885	arg1	step					921:924	the key step	913:924	the key step	913:924	Purification of isolated proteins from the excess of detergent proved to be the key step.
26746104	8	22	theme	proteins	1398:1405	arg1	analysis					1344:1351	N-glycan analysis	1335:1351	N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material	1335:1449	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	9	23	theme	membrane	1610:1617	arg1	proteins					1619:1626	membrane proteins	1610:1626	membrane proteins	1610:1626	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	4	24	theme	detergent	714:722	arg1	X-114					731:735	the non-ionic detergent Triton X-114	700:735	the non-ionic detergent Triton X-114	700:735	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	11	25	theme	Special	1882:1888	arg1	Issue					1890:1894	a Special Issue	1880:1894	a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc	1880:1974	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26746104	11	26	dep	Glycans	1906:1912	arg1	Lauc					1971:1974	Lauc	1971:1974	Lauc	1971:1974	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26746104	6	27	theme	protein	1039:1045	arg1	recoveries					1047:1056	maximum protein recoveries	1031:1056	maximum protein recoveries	1031:1056	Therefore, several purification procedures were tested to efficiently remove detergent, while retaining maximum protein recoveries.
26746104	4	28	theme	liquid	801:806	arg1	HILIC-UPLC					824:833	HILIC-UPLC	824:833	HILIC-UPLC	824:833	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	4	28	theme	liquid	801:806	arg1	chromatography					808:821	hydrophilic interaction liquid chromatography	777:821	hydrophilic interaction liquid chromatography (HILIC-UPLC)	777:834	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	9	29	theme	cell	1680:1683	arg1	types					1685:1689	cell types	1680:1689	cell types	1680:1689	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	4	30	dep	METHODS	599:605	arg1	was					629:631	was	629:631	was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC)	629:834	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	2	31	theme	membrane	290:297	arg1	proteins					299:306	membrane proteins	290:306	membrane proteins	290:306	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	8	32	theme	material	1442:1449	arg1	kinds					1422:1426	different kinds	1412:1426	different kinds of biological material	1412:1449	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	3	33	theme	N-glycans	459:467	arg1	composition					444:454	The composition	440:454	The composition of N-glycans attached to membrane proteins	440:497	The composition of N-glycans attached to membrane proteins has not been sufficiently studied due to the lack of efficient and reproducible analytical methods.
26746104	9	34	dep	CONCLUSIONS	1452:1462	arg1	used					1483:1486	used	1483:1486	be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins	1480:1626	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	5	35	theme	isolated	853:860	arg1	proteins					862:869	isolated proteins	853:869	isolated proteins from the excess of detergent	853:898	Purification of isolated proteins from the excess of detergent proved to be the key step.
26746104	4	36	theme	hydrophilic	777:787	arg1	HILIC-UPLC					824:833	HILIC-UPLC	824:833	HILIC-UPLC	824:833	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	4	36	theme	hydrophilic	777:787	arg1	chromatography					808:821	hydrophilic interaction liquid chromatography	777:821	hydrophilic interaction liquid chromatography (HILIC-UPLC)	777:834	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	10	37	theme	HILIC-UPLC	1790:1799	arg1	profiles					1801:1808	reliable HILIC-UPLC profiles	1781:1808	reliable HILIC-UPLC profiles of N-glycans released from membrane proteins	1781:1853	GENERAL SIGNIFICANCE The simple method was successfully optimised to generate reliable HILIC-UPLC profiles of N-glycans released from membrane proteins.
26746104	0	38	theme	hydrophobic	14:24	arg1	proteins					35:42	hydrophobic membrane proteins	14:42	hydrophobic membrane proteins	14:42	Enrichment of hydrophobic membrane proteins using Triton X-114 and subsequent analysis of their N-glycosylation.
26746104	1	39	theme	BACKGROUND	113:122	arg1	proteins					133:140	BACKGROUND Numerous proteins	113:140	BACKGROUND Numerous proteins depend on correct glycosylation for their proper function and nearly all membrane	113:222	BACKGROUND Numerous proteins depend on correct glycosylation for their proper function and nearly all membrane, as well as secreted, proteins are glycosylated.
26746104	1	40	theme	correct	152:158	arg1	glycosylation					160:172	correct glycosylation	152:172	correct glycosylation	152:172	BACKGROUND Numerous proteins depend on correct glycosylation for their proper function and nearly all membrane, as well as secreted, proteins are glycosylated.
26746104	9	41	attach	attached	1598:1605	arg1	proteins					1619:1626	membrane proteins	1610:1626	membrane proteins	1610:1626	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	9	41	attach	attached	1598:1605	arg2	N-glycans					1588:1596	oligomannose and complex type N-glycans	1558:1596	N-glycans	1588:1596	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	0	42	theme	proteins	35:42	arg1	Enrichment					0:9	Enrichment	0:9	Enrichment of hydrophobic membrane proteins	0:42	Enrichment of hydrophobic membrane proteins using Triton X-114 and subsequent analysis of their N-glycosylation.
26746104	2	43	from	interactions	406:417	arg1	surface					431:437	the cell surface	422:437	the cell surface	422:437	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	7	44	theme	different	1192:1200	arg1	profiles					1211:1218	different N-glycan profiles	1192:1218	different N-glycan profiles of the aforementioned protein groups	1192:1255	RESULTS CPE showed to be an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups.
26746104	2	45	theme	many	332:335	arg1	processes					337:345	many processes	332:345	many processes including the intercellular recognition and intermolecular interactions on the cell surface	332:437	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	2	45	theme	many	332:335	arg1	recognition					375:385	the intercellular recognition and intermolecular interactions	357:417	recognition	375:385	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	2	45	theme	many	332:335	arg1	interactions					406:417	the intercellular recognition and intermolecular interactions	357:417	interactions	406:417	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	3	46	theme	efficient	552:560	arg1	methods					590:596	efficient and reproducible analytical methods	552:596	efficient and reproducible analytical methods	552:596	The composition of N-glycans attached to membrane proteins has not been sufficiently studied due to the lack of efficient and reproducible analytical methods.
26746104	4	47	theme	study	623:627	arg1	aim					611:613	The aim	607:613	The aim of this study	607:627	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	3	48	theme	membrane	481:488	arg1	proteins					490:497	membrane proteins	481:497	membrane proteins	481:497	The composition of N-glycans attached to membrane proteins has not been sufficiently studied due to the lack of efficient and reproducible analytical methods.
26746104	8	49	from	kinds	1422:1426	arg1	proteins					1398:1405	both membrane and intracellular (soluble) proteins	1356:1405	proteins	1398:1405	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	8	49	from	kinds	1422:1426	arg1	analysis					1344:1351	N-glycan analysis	1335:1351	N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material	1335:1449	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	8	49	from	kinds	1422:1426	arg1	membrane					1361:1368	both membrane and intracellular (soluble) proteins	1356:1405	membrane	1361:1368	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	10	50	dep	SIGNIFICANCE	1711:1722	arg1	method					1735:1740	The simple method	1724:1740	GENERAL SIGNIFICANCE The simple method	1703:1740	GENERAL SIGNIFICANCE The simple method was successfully optimised to generate reliable HILIC-UPLC profiles of N-glycans released from membrane proteins.
26746104	2	51	theme	intercellular	361:373	arg1	recognition					375:385	the intercellular recognition and intermolecular interactions	357:417	recognition	375:385	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	6	52	theme	several	938:944	arg1	procedures					959:968	several purification procedures	938:968	several purification procedures	938:968	Therefore, several purification procedures were tested to efficiently remove detergent, while retaining maximum protein recoveries.
26746104	2	53	theme	proteins	299:306	arg1	Glycosylation					273:285	Glycosylation	273:285	Glycosylation of membrane proteins	273:306	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	9	54	theme	analytical	1497:1506	arg1	method					1469:1474	This method	1464:1474	This method	1464:1474	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	9	54	theme	analytical	1497:1506	arg1	tool					1508:1511	a new analytical tool	1491:1511	a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins	1491:1626	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	9	55	used	used	1483:1486	arg2	method					1469:1474	This method	1464:1474	This method	1464:1474	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	9	55	used	used	1483:1486	arg2	tool					1508:1511	a new analytical tool	1491:1511	a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins	1491:1626	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	8	56	theme	different	1412:1420	arg1	kinds					1422:1426	different kinds	1412:1426	different kinds of biological material	1412:1449	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	2	57	gly	Glycosylation	273:285	arg1	proteins					299:306	membrane proteins	290:306	membrane proteins	290:306	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	7	58	theme	soluble	1143:1149	arg1	proteins					1151:1158	soluble proteins	1143:1158	soluble proteins	1143:1158	RESULTS CPE showed to be an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups.
26746104	4	59	theme	proteins	686:693	arg1	CPE					669:671	CPE	669:671	CPE	669:671	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	4	59	theme	proteins	686:693	arg1	extraction					657:666	cloud-point extraction	645:666	cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114	645:735	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	1	60	theme	proper	184:189	arg1	function					191:198	their proper function	178:198	their proper function	178:198	BACKGROUND Numerous proteins depend on correct glycosylation for their proper function and nearly all membrane, as well as secreted, proteins are glycosylated.
26746104	0	61	theme	Triton	50:55	arg1	X-114					57:61	Triton X-114	50:61	Triton X-114	50:61	Enrichment of hydrophobic membrane proteins using Triton X-114 and subsequent analysis of their N-glycosylation.
26746104	3	62	attach	attached	469:476	arg1	proteins					490:497	membrane proteins	481:497	membrane proteins	481:497	The composition of N-glycans attached to membrane proteins has not been sufficiently studied due to the lack of efficient and reproducible analytical methods.
26746104	3	62	attach	attached	469:476	arg2	N-glycans					459:467	N-glycans	459:467	N-glycans attached to membrane proteins	459:497	The composition of N-glycans attached to membrane proteins has not been sufficiently studied due to the lack of efficient and reproducible analytical methods.
26746104	4	63	theme	membrane	677:684	arg1	proteins					686:693	membrane proteins	677:693	membrane proteins	677:693	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	3	64	theme	analytical	579:588	arg1	methods					590:596	efficient and reproducible analytical methods	552:596	efficient and reproducible analytical methods	552:596	The composition of N-glycans attached to membrane proteins has not been sufficiently studied due to the lack of efficient and reproducible analytical methods.
26746104	1	65	theme	all	211:213	arg1	membrane					215:222	nearly all membrane	204:222	nearly all membrane	204:222	BACKGROUND Numerous proteins depend on correct glycosylation for their proper function and nearly all membrane, as well as secreted, proteins are glycosylated.
26746104	4	66	theme	cloud-point	645:655	arg1	CPE					669:671	CPE	669:671	CPE	669:671	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	4	66	theme	cloud-point	645:655	arg1	extraction					657:666	cloud-point extraction	645:666	cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114	645:735	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	10	67	theme	simple	1728:1733	arg1	method					1735:1740	The simple method	1724:1740	GENERAL SIGNIFICANCE The simple method	1703:1740	GENERAL SIGNIFICANCE The simple method was successfully optimised to generate reliable HILIC-UPLC profiles of N-glycans released from membrane proteins.
26746104	9	68	theme	oligomannose	1558:1569	arg1	N-glycans					1588:1596	oligomannose and complex type N-glycans	1558:1596	N-glycans	1588:1596	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	11	69	theme	Guest	1940:1944	arg1	Editor					1946:1951	Guest Editor	1940:1951	Guest Editor	1940:1951	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26746104	9	70	theme	complex	1575:1581	arg1	N-glycans					1588:1596	oligomannose and complex type N-glycans	1558:1596	N-glycans	1588:1596	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	8	71	theme	satisfactory	1288:1299	arg1	reproducibility					1301:1315	reproducibility	1301:1315	reproducibility	1301:1315	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	4	72	theme	Triton	724:729	arg1	X-114					731:735	the non-ionic detergent Triton X-114	700:735	the non-ionic detergent Triton X-114	700:735	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	7	73	theme	RESULTS	1059:1065	arg1	CPE					1067:1069	RESULTS CPE	1059:1069	RESULTS CPE	1059:1069	RESULTS CPE showed to be an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups.
26746104	7	73	theme	RESULTS	1059:1065	arg1	method					1097:1102	an efficient method	1084:1102	an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups	1084:1255	RESULTS CPE showed to be an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups.
26746104	9	74	theme	N-glycans	1588:1596	arg1	detection					1526:1534	detection	1526:1534	detection	1526:1534	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	9	74	theme	N-glycans	1588:1596	arg1	quantification					1540:1553	quantification	1540:1553	quantification	1540:1553	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	0	75	theme	subsequent	67:76	arg1	analysis					78:85	subsequent analysis	67:85	subsequent analysis of their N-glycosylation	67:110	Enrichment of hydrophobic membrane proteins using Triton X-114 and subsequent analysis of their N-glycosylation.
26746104	5	76	theme	key	917:919	arg1	Purification					837:848	Purification	837:848	Purification of isolated proteins from the excess of detergent	837:898	Purification of isolated proteins from the excess of detergent proved to be the key step.
26746104	5	76	theme	key	917:919	arg1	step					921:924	the key step	913:924	the key step	913:924	Purification of isolated proteins from the excess of detergent proved to be the key step.
26746104	9	77	from	differences	1665:1675	arg1	states					1695:1700	states	1695:1700	states	1695:1700	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	9	77	from	differences	1665:1675	arg1	types					1685:1689	cell types	1680:1689	cell types	1680:1689	CONCLUSIONS This method can be used as a new analytical tool for reliable detection and quantification of oligomannose and complex type N-glycans attached to membrane proteins, thus serving to distinguish between differences in cell types and states.
26746104	5	78	from	Purification	837:848	arg1	excess					880:885	the excess	876:885	the excess of detergent	876:898	Purification of isolated proteins from the excess of detergent proved to be the key step.
26746104	8	79	from	analysis	1344:1351	arg1	kinds					1422:1426	different kinds	1412:1426	different kinds of biological material	1412:1449	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	4	80	theme	non-ionic	704:712	arg1	X-114					731:735	the non-ionic detergent Triton X-114	700:735	the non-ionic detergent Triton X-114	700:735	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	11	81	theme	Issue	1890:1894	arg1	part					1872:1875	part	1872:1875	part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc	1872:1974	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26746104	0	82	theme	N-glycosylation	96:110	arg1	analysis					78:85	subsequent analysis	67:85	subsequent analysis of their N-glycosylation	67:110	Enrichment of hydrophobic membrane proteins using Triton X-114 and subsequent analysis of their N-glycosylation.
26746104	0	82	theme	N-glycosylation	96:110	arg1	X-114					57:61	Triton X-114	50:61	Triton X-114	50:61	Enrichment of hydrophobic membrane proteins using Triton X-114 and subsequent analysis of their N-glycosylation.
26746104	6	83	theme	maximum	1031:1037	arg1	recoveries					1047:1056	maximum protein recoveries	1031:1056	maximum protein recoveries	1031:1056	Therefore, several purification procedures were tested to efficiently remove detergent, while retaining maximum protein recoveries.
26746104	4	84	theme	interaction	789:799	arg1	HILIC-UPLC					824:833	HILIC-UPLC	824:833	HILIC-UPLC	824:833	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	4	84	theme	interaction	789:799	arg1	chromatography					808:821	hydrophilic interaction liquid chromatography	777:821	hydrophilic interaction liquid chromatography (HILIC-UPLC)	777:834	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	7	85	theme	groups	1250:1255	arg1	profiles					1211:1218	different N-glycan profiles	1192:1218	different N-glycan profiles of the aforementioned protein groups	1192:1255	RESULTS CPE showed to be an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups.
26746104	8	86	theme	biological	1431:1440	arg1	material					1442:1449	biological material	1431:1449	biological material	1431:1449	The resulting protocol showed satisfactory reproducibility and potential for N-glycan analysis of both membrane and intracellular (soluble) proteins from different kinds of biological material.
26746104	7	87	theme	aforementioned	1227:1240	arg1	groups					1250:1255	the aforementioned protein groups	1223:1255	the aforementioned protein groups	1223:1255	RESULTS CPE showed to be an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups.
26746104	2	88	theme	intermolecular	391:404	arg1	interactions					406:417	the intercellular recognition and intermolecular interactions	357:417	interactions	406:417	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	0	89	theme	membrane	26:33	arg1	proteins					35:42	hydrophobic membrane proteins	14:42	hydrophobic membrane proteins	14:42	Enrichment of hydrophobic membrane proteins using Triton X-114 and subsequent analysis of their N-glycosylation.
26746104	1	90	gly	glycosylated	259:270	arg1	proteins					133:140	BACKGROUND Numerous proteins	113:140	BACKGROUND Numerous proteins depend on correct glycosylation for their proper function and nearly all membrane	113:222	BACKGROUND Numerous proteins depend on correct glycosylation for their proper function and nearly all membrane, as well as secreted, proteins are glycosylated.
26746104	1	90	gly	glycosylated	259:270	arg1	proteins					246:253	proteins	246:253	proteins	246:253	BACKGROUND Numerous proteins depend on correct glycosylation for their proper function and nearly all membrane, as well as secreted, proteins are glycosylated.
26746104	10	91	theme	reliable	1781:1788	arg1	profiles					1801:1808	reliable HILIC-UPLC profiles	1781:1808	reliable HILIC-UPLC profiles of N-glycans released from membrane proteins	1781:1853	GENERAL SIGNIFICANCE The simple method was successfully optimised to generate reliable HILIC-UPLC profiles of N-glycans released from membrane proteins.
26746104	7	92	theme	N-glycan	1202:1209	arg1	profiles					1211:1218	different N-glycan profiles	1192:1218	different N-glycan profiles of the aforementioned protein groups	1192:1255	RESULTS CPE showed to be an efficient method to simultaneously extract membrane and soluble proteins, which subsequently resulted in different N-glycan profiles of the aforementioned protein groups.
26746104	2	93	theme	cell	426:429	arg1	surface					431:437	the cell surface	422:437	the cell surface	422:437	Glycosylation of membrane proteins plays a crucial role in many processes including the intercellular recognition and intermolecular interactions on the cell surface.
26746104	10	94	attach	released	1823:1830	arg1	proteins					1846:1853	membrane proteins	1837:1853	membrane proteins	1837:1853	GENERAL SIGNIFICANCE The simple method was successfully optimised to generate reliable HILIC-UPLC profiles of N-glycans released from membrane proteins.
26746104	10	94	attach	released	1823:1830	arg2	N-glycans					1813:1821	N-glycans	1813:1821	N-glycans released from membrane proteins	1813:1853	GENERAL SIGNIFICANCE The simple method was successfully optimised to generate reliable HILIC-UPLC profiles of N-glycans released from membrane proteins.
26746104	10	95	theme	N-glycans	1813:1821	arg1	profiles					1801:1808	reliable HILIC-UPLC profiles	1781:1808	reliable HILIC-UPLC profiles of N-glycans released from membrane proteins	1781:1853	GENERAL SIGNIFICANCE The simple method was successfully optimised to generate reliable HILIC-UPLC profiles of N-glycans released from membrane proteins.
26746104	1	96	dep	proteins	133:140	arg1	depend					142:147	depend	142:147	depend on correct glycosylation for their proper function and nearly all membrane	142:222	BACKGROUND Numerous proteins depend on correct glycosylation for their proper function and nearly all membrane, as well as secreted, proteins are glycosylated.
26746104	4	97	with	extraction	657:666	arg1	X-114					731:735	the non-ionic detergent Triton X-114	700:735	the non-ionic detergent Triton X-114	700:735	METHODS The aim of this study was to optimise cloud-point extraction (CPE) of membrane proteins with the non-ionic detergent Triton X-114 and analyse their N-glycosylation using hydrophilic interaction liquid chromatography (HILIC-UPLC).
26746104	10	98	theme	membrane	1837:1844	arg1	proteins					1846:1853	membrane proteins	1837:1853	membrane proteins	1837:1853	GENERAL SIGNIFICANCE The simple method was successfully optimised to generate reliable HILIC-UPLC profiles of N-glycans released from membrane proteins.
23808670	1	0	from	scaffold	358:365	arg1	features					255:262	the main features	246:262	the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold	246:365	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	2	1	theme	structural	453:462	arg1	ions					464:467	structural ions	453:467	structural ions	453:467	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	4	2	theme	such	754:757	arg1	ion					770:772	such structural ion	754:772	such structural ion	754:772	Such flexibility pattern is not properly maintained in the absence of such structural ion or when the ion is replaced by Ca(2+).
23808670	6	3	theme	inhibitory	952:961	arg1	effect					963:968	the experimentally reported inhibitory effect	924:968	the experimentally reported inhibitory effect of calcium over hepII	924:990	These events correlate with the experimentally reported inhibitory effect of calcium over hepII.
23808670	7	4	theme	hepII	1077:1081	arg1	dynamics					1083:1090	hepII dynamics	1077:1090	hepII dynamics	1077:1090	Additionally, the glycan chain seems able to promote an additional stabilization on hepII dynamics.
23808670	1	5	theme	role	303:306	arg1	features					255:262	the main features	246:262	the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold	246:365	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	2	6	theme	glycosylated	544:555	arg1	hepII					557:561	glycosylated hepII	544:561	glycosylated hepII with Zn(2+)	544:573	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	6	7	theme	reported	943:950	arg1	effect					963:968	the experimentally reported inhibitory effect	924:968	the experimentally reported inhibitory effect of calcium over hepII	924:990	These events correlate with the experimentally reported inhibitory effect of calcium over hepII.
23808670	7	8	theme	additional	1049:1058	arg1	stabilization					1060:1072	an additional stabilization	1046:1072	an additional stabilization on hepII dynamics	1046:1090	Additionally, the glycan chain seems able to promote an additional stabilization on hepII dynamics.
23808670	1	9	from	dynamics	278:285	arg1	scaffold					358:365	the protein scaffold	346:365	the protein scaffold	346:365	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	1	10	from	Pedobacter	152:161	arg1	heparinase					125:134	heparinase II	125:137	heparinase II (hepII) from Pedobacter heparinus	125:171	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	1	10	from	Pedobacter	152:161	arg1	hepII					140:144	hepII	140:144	hepII	140:144	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	1	10	from	Pedobacter	152:161	arg1	evaluation					111:120	a conformational evaluation	94:120	a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus	94:171	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	4	11	theme	structural	759:768	arg1	ion					770:772	such structural ion	754:772	such structural ion	754:772	Such flexibility pattern is not properly maintained in the absence of such structural ion or when the ion is replaced by Ca(2+).
23808670	8	12	theme	hepII	1193:1197	arg1	features					1181:1188	the structural and dynamical features	1152:1188	the structural and dynamical features of hepII	1152:1197	Taken together, these results improve our understanding of the structural and dynamical features of hepII, as well as atomic-level comprehension of previous experimental data.
23808670	1	13	theme	main	250:253	arg1	features					255:262	the main features	246:262	the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold	246:365	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	3	14	theme	specific	661:668	arg1	regions					675:681	specific loop regions	661:681	specific loop regions	661:681	The obtained data suggest a role for Zn(2+) in modulating the protein flexibility at specific loop regions.
23808670	1	15	theme	glycan	315:320	arg1	components					332:341	the glycan and metal components	311:341	components	332:341	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	1	16	theme	current	72:78	arg1	work					80:83	The current work	68:83	The current work	68:83	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	2	17	theme	nonglycosylated	470:484	arg1	hepII					486:490	nonglycosylated hepII	470:490	nonglycosylated hepII with Zn(2+)	470:502	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	18	with	hepII	486:490	arg1	Zn					568:569	Zn	568:569	Zn(2+)	568:573	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	18	with	hepII	486:490	arg1	Zn					497:498	Zn	497:498	Zn(2+)	497:502	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	18	with	hepII	486:490	arg1	2+					571:572	2+	571:572	2+	571:572	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	18	with	hepII	486:490	arg1	Ca					532:533	Ca	532:533	Ca(2+)	532:537	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	18	with	hepII	486:490	arg1	2+					500:501	2+	500:501	2+	500:501	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	18	with	hepII	486:490	arg1	2+					535:536	2+	535:536	2+	535:536	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	1	19	theme	metal	326:330	arg1	components					332:341	the glycan and metal components	311:341	components	332:341	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	1	20	from	features	255:262	arg1	scaffold					358:365	the protein scaffold	346:365	the protein scaffold	346:365	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	5	21	theme	favorable	860:868	arg1	interactions					870:881	more favorable interactions	855:881	more favorable interactions with Zn(2+)	855:893	Still, semiempirical calculations suggest more favorable interactions with Zn(2+).
23808670	0	22	from	Pedobacter	46:55	arg1	glycobiology					11:22	Structural glycobiology	0:22	Structural glycobiology of heparinase II from Pedobacter heparinus	0:65	Structural glycobiology of heparinase II from Pedobacter heparinus.
23808670	0	23	theme	Structural	0:9	arg1	glycobiology					11:22	Structural glycobiology	0:22	Structural glycobiology of heparinase II from Pedobacter heparinus	0:65	Structural glycobiology of heparinase II from Pedobacter heparinus.
23808670	1	24	dep	Pedobacter	152:161	arg1	heparinus					163:171	Pedobacter heparinus	152:171	Pedobacter heparinus	152:171	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	1	25	theme	molecular	184:192	arg1	simulations					208:218	molecular dynamics (MD) simulations	184:218	molecular dynamics (MD) simulations	184:218	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	0	26	theme	heparinase	27:36	arg1	glycobiology					11:22	Structural glycobiology	0:22	Structural glycobiology of heparinase II from Pedobacter heparinus	0:65	Structural glycobiology of heparinase II from Pedobacter heparinus.
23808670	3	27	theme	protein	638:644	arg1	flexibility					646:656	the protein flexibility	634:656	the protein flexibility	634:656	The obtained data suggest a role for Zn(2+) in modulating the protein flexibility at specific loop regions.
23808670	1	28	theme	conformational	96:109	arg1	evaluation					111:120	a conformational evaluation	94:120	a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus	94:171	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	8	29	theme	data	1263:1266	arg1	comprehension					1224:1236	atomic-level comprehension	1211:1236	atomic-level comprehension of previous experimental data	1211:1266	Taken together, these results improve our understanding of the structural and dynamical features of hepII, as well as atomic-level comprehension of previous experimental data.
23808670	8	29	theme	data	1263:1266	arg1	understanding					1135:1147	our understanding	1131:1147	our understanding of the structural and dynamical features of hepII	1131:1197	Taken together, these results improve our understanding of the structural and dynamical features of hepII, as well as atomic-level comprehension of previous experimental data.
23808670	1	30	theme	dynamics	194:201	arg1	simulations					208:218	molecular dynamics (MD) simulations	184:218	molecular dynamics (MD) simulations	184:218	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	4	31	dep	maintained	725:734	arg1	replaced					793:800	replaced	793:800	is replaced by Ca(2+)	790:810	Such flexibility pattern is not properly maintained in the absence of such structural ion or when the ion is replaced by Ca(2+).
23808670	4	31	dep	maintained	725:734	arg1	absence					743:749	the absence	739:749	the absence of such structural ion	739:772	Such flexibility pattern is not properly maintained in the absence of such structural ion or when the ion is replaced by Ca(2+).
23808670	2	32	gly	nonglycosylated	470:484	arg1	hepII					486:490	nonglycosylated hepII	470:490	nonglycosylated hepII with Zn(2+)	470:502	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	1	33	theme	components	332:341	arg1	role					303:306	the role	299:306	the role of the glycan and metal components on the protein scaffold	299:365	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	1	33	theme	components	332:341	arg1	dynamics					278:285	the enzyme dynamics	267:285	the enzyme dynamics	267:285	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	2	34	with	hepII	557:561	arg1	Zn					568:569	Zn	568:569	Zn(2+)	568:573	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	34	with	hepII	557:561	arg1	Zn					497:498	Zn	497:498	Zn(2+)	497:502	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	34	with	hepII	557:561	arg1	2+					571:572	2+	571:572	2+	571:572	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	34	with	hepII	557:561	arg1	Ca					532:533	Ca	532:533	Ca(2+)	532:537	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	34	with	hepII	557:561	arg1	2+					500:501	2+	500:501	2+	500:501	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	34	with	hepII	557:561	arg1	2+					535:536	2+	535:536	2+	535:536	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	1	35	from	role	303:306	arg1	scaffold					358:365	the protein scaffold	346:365	the protein scaffold	346:365	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	1	36	theme	enzyme	271:276	arg1	dynamics					278:285	the enzyme dynamics	267:285	the enzyme dynamics	267:285	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	2	37	gly	nonglycosylated	423:437	arg1	hepII					439:443	nonglycosylated hepII	423:443	nonglycosylated hepII without structural ions	423:467	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	38	gly	glycosylated	544:555	arg1	hepII					557:561	glycosylated hepII	544:561	glycosylated hepII with Zn(2+)	544:573	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	1	39	theme	MD	204:205	arg1	simulations					208:218	molecular dynamics (MD) simulations	184:218	molecular dynamics (MD) simulations	184:218	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	2	40	theme	nonglycosylated	423:437	arg1	hepII					439:443	nonglycosylated hepII	423:443	nonglycosylated hepII without structural ions	423:467	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	1	41	theme	dynamics	278:285	arg1	features					255:262	the main features	246:262	the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold	246:365	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	7	42	theme	glycan	1011:1016	arg1	chain					1018:1022	the glycan chain	1007:1022	the glycan chain	1007:1022	Additionally, the glycan chain seems able to promote an additional stabilization on hepII dynamics.
23808670	0	43	dep	Pedobacter	46:55	arg1	heparinus					57:65	Pedobacter heparinus	46:65	Pedobacter heparinus	46:65	Structural glycobiology of heparinase II from Pedobacter heparinus.
23808670	8	44	theme	structural	1156:1165	arg1	features					1181:1188	the structural and dynamical features	1152:1188	the structural and dynamical features of hepII	1152:1197	Taken together, these results improve our understanding of the structural and dynamical features of hepII, as well as atomic-level comprehension of previous experimental data.
23808670	3	45	theme	loop	670:673	arg1	regions					675:681	specific loop regions	661:681	specific loop regions	661:681	The obtained data suggest a role for Zn(2+) in modulating the protein flexibility at specific loop regions.
23808670	4	46	theme	ion	770:772	arg1	absence					743:749	the absence	739:749	the absence of such structural ion	739:772	Such flexibility pattern is not properly maintained in the absence of such structural ion or when the ion is replaced by Ca(2+).
23808670	1	47	theme	protein	350:356	arg1	scaffold					358:365	the protein scaffold	346:365	the protein scaffold	346:365	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	8	48	theme	atomic-level	1211:1222	arg1	comprehension					1224:1236	atomic-level comprehension	1211:1236	atomic-level comprehension of previous experimental data	1211:1266	Taken together, these results improve our understanding of the structural and dynamical features of hepII, as well as atomic-level comprehension of previous experimental data.
23808670	3	49	theme	obtained	580:587	arg1	data					589:592	The obtained data	576:592	The obtained data	576:592	The obtained data suggest a role for Zn(2+) in modulating the protein flexibility at specific loop regions.
23808670	1	50	theme	heparinase	125:134	arg1	evaluation					111:120	a conformational evaluation	94:120	a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus	94:171	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	8	51	theme	experimental	1250:1261	arg1	data					1263:1266	previous experimental data	1241:1266	previous experimental data	1241:1266	Taken together, these results improve our understanding of the structural and dynamical features of hepII, as well as atomic-level comprehension of previous experimental data.
23808670	2	52	gly	nonglycosylated	505:519	arg1	hepII					521:525	nonglycosylated hepII	505:525	nonglycosylated hepII with Ca(2+)	505:537	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	1	53	from	evaluation	111:120	arg1	Pedobacter					152:161	Pedobacter	152:161	Pedobacter	152:161	The current work presents a conformational evaluation of heparinase II (hepII) from Pedobacter heparinus, employing molecular dynamics (MD) simulations, in order to characterize the main features of the enzyme dynamics, as well as the role of the glycan and metal components on the protein scaffold.
23808670	2	54	theme	nonglycosylated	505:519	arg1	hepII					521:525	nonglycosylated hepII	505:525	nonglycosylated hepII with Ca(2+)	505:537	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	7	55	from	stabilization	1060:1072	arg1	dynamics					1083:1090	hepII dynamics	1077:1090	hepII dynamics	1077:1090	Additionally, the glycan chain seems able to promote an additional stabilization on hepII dynamics.
23808670	6	56	theme	calcium	973:979	arg1	effect					963:968	the experimentally reported inhibitory effect	924:968	the experimentally reported inhibitory effect of calcium over hepII	924:990	These events correlate with the experimentally reported inhibitory effect of calcium over hepII.
23808670	5	57	theme	semiempirical	820:832	arg1	calculations					834:845	semiempirical calculations	820:845	semiempirical calculations	820:845	Still, semiempirical calculations suggest more favorable interactions with Zn(2+).
23808670	4	58	theme	flexibility	689:699	arg1	pattern					701:707	Such flexibility pattern	684:707	Such flexibility pattern	684:707	Such flexibility pattern is not properly maintained in the absence of such structural ion or when the ion is replaced by Ca(2+).
23808670	8	59	theme	features	1181:1188	arg1	comprehension					1224:1236	atomic-level comprehension	1211:1236	atomic-level comprehension of previous experimental data	1211:1266	Taken together, these results improve our understanding of the structural and dynamical features of hepII, as well as atomic-level comprehension of previous experimental data.
23808670	8	59	theme	features	1181:1188	arg1	understanding					1135:1147	our understanding	1131:1147	our understanding of the structural and dynamical features of hepII	1131:1197	Taken together, these results improve our understanding of the structural and dynamical features of hepII, as well as atomic-level comprehension of previous experimental data.
23808670	8	60	theme	previous	1241:1248	arg1	data					1263:1266	previous experimental data	1241:1266	previous experimental data	1241:1266	Taken together, these results improve our understanding of the structural and dynamical features of hepII, as well as atomic-level comprehension of previous experimental data.
23808670	5	61	with	interactions	870:881	arg1	Zn					888:889	Zn	888:889	Zn(2+)	888:893	Still, semiempirical calculations suggest more favorable interactions with Zn(2+).
23808670	5	61	with	interactions	870:881	arg1	2+					891:892	2+	891:892	2+	891:892	Still, semiempirical calculations suggest more favorable interactions with Zn(2+).
23808670	4	62	theme	Such	684:687	arg1	pattern					701:707	Such flexibility pattern	684:707	Such flexibility pattern	684:707	Such flexibility pattern is not properly maintained in the absence of such structural ion or when the ion is replaced by Ca(2+).
23808670	2	63	with	hepII	439:443	arg1	Zn					568:569	Zn	568:569	Zn(2+)	568:573	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	63	with	hepII	439:443	arg1	Zn					497:498	Zn	497:498	Zn(2+)	497:502	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	63	with	hepII	439:443	arg1	2+					571:572	2+	571:572	2+	571:572	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	63	with	hepII	439:443	arg1	Ca					532:533	Ca	532:533	Ca(2+)	532:537	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	63	with	hepII	439:443	arg1	2+					500:501	2+	500:501	2+	500:501	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	63	with	hepII	439:443	arg1	2+					535:536	2+	535:536	2+	535:536	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	8	64	theme	dynamical	1171:1179	arg1	features					1181:1188	the structural and dynamical features	1152:1188	the structural and dynamical features of hepII	1152:1197	Taken together, these results improve our understanding of the structural and dynamical features of hepII, as well as atomic-level comprehension of previous experimental data.
23808670	2	65	with	hepII	521:525	arg1	Zn					568:569	Zn	568:569	Zn(2+)	568:573	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	65	with	hepII	521:525	arg1	Zn					497:498	Zn	497:498	Zn(2+)	497:502	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	65	with	hepII	521:525	arg1	2+					571:572	2+	571:572	2+	571:572	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	65	with	hepII	521:525	arg1	Ca					532:533	Ca	532:533	Ca(2+)	532:537	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	65	with	hepII	521:525	arg1	2+					500:501	2+	500:501	2+	500:501	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
23808670	2	65	with	hepII	521:525	arg1	2+					535:536	2+	535:536	2+	535:536	Accordingly, four systems were simulated, encompassing nonglycosylated hepII without structural ions, nonglycosylated hepII with Zn(2+), nonglycosylated hepII with Ca(2+), and glycosylated hepII with Zn(2+).
26331458	8	0	theme	D+U	1373:1375	arg1	fractions					1377:1385	the W-S and D+U fractions	1361:1385	the W-S and D+U fractions of starch determined with both methods	1361:1424	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods can led to different predicted values for the effective rumen starch degradability.
26331458	1	1	theme	W	210:210	arg1	fraction					213:220	the washout (W) fraction	197:220	the washout (W) fraction	197:220	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	5	2	from	W-S	783:785	arg1	W					688:688	the W	684:688	the W	684:688	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	7	3	theme	rinsing	1190:1196	arg1	procedures					1198:1207	different rinsing procedures	1180:1207	different rinsing procedures (washing vs. shaking)	1180:1229	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	4	theme	different	1135:1143	arg1	solvents					1286:1293	different solvents	1276:1293	different solvents (water vs. buffer solution)	1276:1321	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	4	theme	different	1135:1143	arg1	duration					1232:1239	duration	1232:1239	duration of rinsing (40 min vs. 60 min)	1232:1270	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	4	theme	different	1135:1143	arg1	approaches					1160:1169	different methodological approaches	1135:1169	different methodological approaches	1135:1169	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	4	theme	different	1135:1143	arg1	procedures					1198:1207	different rinsing procedures	1180:1207	different rinsing procedures (washing vs. shaking)	1180:1229	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	9	5	theme	identical	1588:1596	arg1	conditions					1626:1635	identical and controlled experimental conditions	1588:1635	identical and controlled experimental conditions	1588:1635	In conclusion, the MM with one recommended shaking procedure, performed under identical and controlled experimental conditions, can give more reliable results compared to the WMM, using different washing programs and procedures.
26331458	9	6	with	MM	1529:1530	arg1	procedure					1561:1569	one recommended shaking procedure	1537:1569	one recommended shaking procedure	1537:1569	In conclusion, the MM with one recommended shaking procedure, performed under identical and controlled experimental conditions, can give more reliable results compared to the WMM, using different washing programs and procedures.
26331458	5	7	theme	soluble	691:697	arg1	fractions					711:719	soluble (S) and D+U fractions	691:719	soluble (S) and D+U fractions of N of maize and grass silages	691:751	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	9	8	theme	controlled	1602:1611	arg1	conditions					1626:1635	identical and controlled experimental conditions	1588:1635	identical and controlled experimental conditions	1588:1635	In conclusion, the MM with one recommended shaking procedure, performed under identical and controlled experimental conditions, can give more reliable results compared to the WMM, using different washing programs and procedures.
26331458	5	9	from	fractions	796:804	arg1	W					688:688	the W	684:688	the W	684:688	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	1	10	theme	machine	163:169	arg1	WMM					179:181	WMM	179:181	WMM	179:181	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	1	10	theme	machine	163:169	arg1	method					171:176	a washing machine method	153:176	a washing machine method (WMM)	153:182	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	5	11	from	range	675:679	arg1	W					688:688	the W	684:688	the W	684:688	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	1	12	theme	bag	105:107	arg1	technique					109:117	in situ nylon bag technique	91:117	in situ nylon bag technique	91:117	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	7	13	theme	silages	1105:1111	arg1	starch					1089:1094	starch	1089:1094	starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution)	1089:1321	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	3	14	theme	starch	476:481	arg1	fractions					443:451	the W and non-washout (D+U) fractions	415:451	the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages	415:508	The aim of this study was to determine and compare the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages, using the WMM and the MM.
26331458	5	15	theme	starch	809:814	arg1	W-S					783:785	the W, insoluble washout (W-S) and D+U fractions	757:804	W-S	783:785	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	5	15	theme	starch	809:814	arg1	range					675:679	a large range	667:679	a large range in the W	667:688	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	5	15	theme	starch	809:814	arg1	fractions					711:719	soluble (S) and D+U fractions	691:719	soluble (S) and D+U fractions of N of maize and grass silages	691:751	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	5	15	theme	starch	809:814	arg1	fractions					796:804	the W, insoluble washout (W-S) and D+U fractions	757:804	fractions	796:804	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	8	16	theme	predicted	1447:1455	arg1	values					1457:1462	different predicted values	1437:1462	different predicted values for the effective rumen starch degradability	1437:1507	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods can led to different predicted values for the effective rumen starch degradability.
26331458	5	17	theme	N	724:724	arg1	W-S					783:785	the W, insoluble washout (W-S) and D+U fractions	757:804	W-S	783:785	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	5	17	theme	N	724:724	arg1	range					675:679	a large range	667:679	a large range in the W	667:688	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	5	17	theme	N	724:724	arg1	fractions					711:719	soluble (S) and D+U fractions	691:719	soluble (S) and D+U fractions of N of maize and grass silages	691:751	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	5	17	theme	N	724:724	arg1	fractions					796:804	the W, insoluble washout (W-S) and D+U fractions	757:804	fractions	796:804	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	7	18	theme	buffer	1306:1311	arg1	solution					1313:1320	water vs. buffer solution	1296:1320	solution	1313:1320	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	18	theme	buffer	1306:1311	arg1	solvents					1286:1293	different solvents	1276:1293	different solvents (water vs. buffer solution)	1276:1321	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	6	19	theme	silages	956:962	arg1	fractions					927:935	N fractions	925:935	N fractions of maize and grass silages obtained with both methods	925:989	The values for N fractions of maize and grass silages obtained with both methods were found different (p < 0.001).
26331458	4	20	theme	silage	575:580	arg1	samples					582:588	Ninety-nine maize silage and 99 grass silage samples	537:588	samples	582:588	Ninety-nine maize silage and 99 grass silage samples were selected with a broad range in chemical composition.
26331458	8	21	theme	different	1437:1445	arg1	values					1457:1462	different predicted values	1437:1462	different predicted values for the effective rumen starch degradability	1437:1507	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods can led to different predicted values for the effective rumen starch degradability.
26331458	7	22	located	found	1060:1064	arg2	differences					1031:1041	Large differences	1025:1041	Large differences (p < 0.001)	1025:1053	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	22	located	found	1060:1064	arg1	fraction					1077:1084	the D+U fraction	1069:1084	the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution)	1069:1321	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	22	located	found	1060:1064	arg2	p < 0.001					1044:1052	p < 0.001	1044:1052	p < 0.001	1044:1052	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	3	23	theme	grass	496:500	arg1	silages					502:508	grass silages	496:508	grass silages	496:508	The aim of this study was to determine and compare the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages, using the WMM and the MM.
26331458	5	24	theme	maize	729:733	arg1	N					724:724	N	724:724	N of maize and grass silages	724:751	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	7	25	theme	water	1296:1300	arg1	solution					1313:1320	water vs. buffer solution	1296:1320	solution	1313:1320	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	25	theme	water	1296:1300	arg1	solvents					1286:1293	different solvents	1276:1293	different solvents (water vs. buffer solution)	1276:1321	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	26	theme	D+U	1073:1075	arg1	fraction					1077:1084	the D+U fraction	1069:1084	the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution)	1069:1321	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	4	27	theme	Ninety-nine	537:547	arg1	silage					555:560	Ninety-nine maize silage and 99 grass silage samples	537:588	silage	555:560	Ninety-nine maize silage and 99 grass silage samples were selected with a broad range in chemical composition.
26331458	5	28	theme	large	669:673	arg1	range					675:679	a large range	667:679	a large range in the W	667:688	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	8	29	theme	effective	1472:1480	arg1	degradability					1495:1507	the effective rumen starch degradability	1468:1507	the effective rumen starch degradability	1468:1507	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods can led to different predicted values for the effective rumen starch degradability.
26331458	1	30	theme	feed	125:128	arg1	systems					141:147	many feed evaluation systems	120:147	many feed evaluation systems	120:147	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	5	31	theme	maize	819:823	arg1	silages					825:831	maize silages	819:831	maize silages	819:831	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	8	32	theme	starch	1488:1493	arg1	degradability					1495:1507	the effective rumen starch degradability	1468:1507	the effective rumen starch degradability	1468:1507	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods can led to different predicted values for the effective rumen starch degradability.
26331458	7	33	theme	starch	1089:1094	arg1	fraction					1077:1084	the D+U fraction	1069:1084	the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution)	1069:1321	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	4	34	theme	broad	611:615	arg1	range					617:621	a broad range	609:621	a broad range in chemical composition	609:645	Ninety-nine maize silage and 99 grass silage samples were selected with a broad range in chemical composition.
26331458	2	35	contain	has	281:283	arg1	method					274:279	this method	269:279	this method	269:279	As this method has some disadvantages, an alternate modified method (MM) was recently introduced.
26331458	2	35	contain	has	281:283	arg2	disadvantages					290:302	some disadvantages	285:302	some disadvantages	285:302	As this method has some disadvantages, an alternate modified method (MM) was recently introduced.
26331458	5	36	theme	W	761:761	arg1	W-S					783:785	the W, insoluble washout (W-S) and D+U fractions	757:804	W-S	783:785	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	9	37	theme	washing	1706:1712	arg1	programs					1714:1721	different washing programs	1696:1721	different washing programs	1696:1721	In conclusion, the MM with one recommended shaking procedure, performed under identical and controlled experimental conditions, can give more reliable results compared to the WMM, using different washing programs and procedures.
26331458	5	38	from	fractions	711:719	arg1	W					688:688	the W	684:688	the W	684:688	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	5	39	theme	due	862:864	arg1	starch					809:814	starch	809:814	starch	809:814	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	5	40	theme	insoluble	764:772	arg1	W-S					783:785	the W, insoluble washout (W-S) and D+U fractions	757:804	W-S	783:785	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	2	41	theme	alternate	308:316	arg1	MM					335:336	MM	335:336	MM	335:336	As this method has some disadvantages, an alternate modified method (MM) was recently introduced.
26331458	2	41	theme	alternate	308:316	arg1	method					327:332	an alternate modified method	305:332	an alternate modified method (MM)	305:337	As this method has some disadvantages, an alternate modified method (MM) was recently introduced.
26331458	5	42	theme	grass	739:743	arg1	silages					745:751	grass silages	739:751	grass silages	739:751	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	0	43	theme	fractionation	14:26	arg1	methods					28:34	fractionation methods	14:34	fractionation methods	14:34	Comparison of fractionation methods for nitrogen and starch in maize and grass silages.
26331458	6	44	theme	N	925:925	arg1	fractions					927:935	N fractions	925:935	N fractions of maize and grass silages obtained with both methods	925:989	The values for N fractions of maize and grass silages obtained with both methods were found different (p < 0.001).
26331458	1	45	theme	in situ	91:97	arg1	technique					109:117	in situ nylon bag technique	91:117	in situ nylon bag technique	91:117	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	5	46	theme	D+U	792:794	arg1	fractions					796:804	the W, insoluble washout (W-S) and D+U fractions	757:804	fractions	796:804	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	1	47	dep	use	149:151	arg1	wash					229:232	wash	229:232	to wash the rumen incubated nylon bags	226:263	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	1	47	dep	use	149:151	arg1	determine					187:195	determine	187:195	to determine the washout (W) fraction	184:220	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	1	48	theme	nylon	254:258	arg1	bags					260:263	the rumen incubated nylon bags	234:263	the rumen incubated nylon bags	234:263	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	7	49	theme	Large	1025:1029	arg1	p < 0.001					1044:1052	p < 0.001	1044:1052	p < 0.001	1044:1052	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	49	theme	Large	1025:1029	arg1	differences					1031:1041	Large differences	1025:1041	Large differences (p < 0.001)	1025:1053	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	3	50	theme	maize	486:490	arg1	fractions					443:451	the W and non-washout (D+U) fractions	415:451	the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages	415:508	The aim of this study was to determine and compare the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages, using the WMM and the MM.
26331458	7	51	theme	shaking	1222:1228	arg1	procedures					1198:1207	different rinsing procedures	1180:1207	different rinsing procedures (washing vs. shaking)	1180:1229	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	3	52	theme	W	419:419	arg1	fractions					443:451	the W and non-washout (D+U) fractions	415:451	the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages	415:508	The aim of this study was to determine and compare the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages, using the WMM and the MM.
26331458	7	53	theme	washing	1210:1216	arg1	procedures					1198:1207	different rinsing procedures	1180:1207	different rinsing procedures (washing vs. shaking)	1180:1229	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	9	54	theme	shaking	1553:1559	arg1	procedure					1561:1569	one recommended shaking procedure	1537:1569	one recommended shaking procedure	1537:1569	In conclusion, the MM with one recommended shaking procedure, performed under identical and controlled experimental conditions, can give more reliable results compared to the WMM, using different washing programs and procedures.
26331458	6	55	dep	different	1002:1010	arg1	p < 0.001					1013:1021	p < 0.001	1013:1021	p < 0.001	1013:1021	The values for N fractions of maize and grass silages obtained with both methods were found different (p < 0.001).
26331458	7	56	theme	different	1180:1188	arg1	procedures					1198:1207	different rinsing procedures	1180:1207	different rinsing procedures (washing vs. shaking)	1180:1229	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	5	57	theme	D+U	707:709	arg1	fractions					711:719	soluble (S) and D+U fractions	691:719	soluble (S) and D+U fractions of N of maize and grass silages	691:751	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	8	58	theme	large	1328:1332	arg1	p < 0.001					1347:1355	p < 0.001	1347:1355	p < 0.001	1347:1355	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods can led to different predicted values for the effective rumen starch degradability.
26331458	8	58	theme	large	1328:1332	arg1	differences					1334:1344	The large differences	1324:1344	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods	1324:1424	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods can led to different predicted values for the effective rumen starch degradability.
26331458	3	59	theme	non-washout	425:435	arg1	fractions					443:451	the W and non-washout (D+U) fractions	415:451	the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages	415:508	The aim of this study was to determine and compare the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages, using the WMM and the MM.
26331458	7	60	theme	methodological	1145:1158	arg1	solvents					1286:1293	different solvents	1276:1293	different solvents (water vs. buffer solution)	1276:1321	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	60	theme	methodological	1145:1158	arg1	duration					1232:1239	duration	1232:1239	duration of rinsing (40 min vs. 60 min)	1232:1270	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	60	theme	methodological	1145:1158	arg1	approaches					1160:1169	different methodological approaches	1135:1169	different methodological approaches	1135:1169	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	60	theme	methodological	1145:1158	arg1	procedures					1198:1207	different rinsing procedures	1180:1207	different rinsing procedures (washing vs. shaking)	1180:1229	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	3	61	theme	nitrogen	456:463	arg1	fractions					443:451	the W and non-washout (D+U) fractions	415:451	the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages	415:508	The aim of this study was to determine and compare the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages, using the WMM and the MM.
26331458	8	62	theme	starch	1390:1395	arg1	fractions					1377:1385	the W-S and D+U fractions	1361:1385	the W-S and D+U fractions of starch determined with both methods	1361:1424	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods can led to different predicted values for the effective rumen starch degradability.
26331458	1	63	theme	washing	155:161	arg1	WMM					179:181	WMM	179:181	WMM	179:181	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	1	63	theme	washing	155:161	arg1	method					171:176	a washing machine method	153:176	a washing machine method (WMM)	153:182	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	9	64	theme	experimental	1613:1624	arg1	conditions					1626:1635	identical and controlled experimental conditions	1588:1635	identical and controlled experimental conditions	1588:1635	In conclusion, the MM with one recommended shaking procedure, performed under identical and controlled experimental conditions, can give more reliable results compared to the WMM, using different washing programs and procedures.
26331458	8	65	from	differences	1334:1344	arg1	fractions					1377:1385	the W-S and D+U fractions	1361:1385	the W-S and D+U fractions of starch determined with both methods	1361:1424	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods can led to different predicted values for the effective rumen starch degradability.
26331458	1	66	theme	nylon	99:103	arg1	technique					109:117	in situ nylon bag technique	91:117	in situ nylon bag technique	91:117	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	7	67	theme	maize	1099:1103	arg1	silages					1105:1111	maize silages	1099:1111	maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution)	1099:1321	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	9	68	theme	reliable	1652:1659	arg1	results					1661:1667	more reliable results	1647:1667	more reliable results	1647:1667	In conclusion, the MM with one recommended shaking procedure, performed under identical and controlled experimental conditions, can give more reliable results compared to the WMM, using different washing programs and procedures.
26331458	4	69	from	range	617:621	arg1	composition					635:645	chemical composition	626:645	chemical composition	626:645	Ninety-nine maize silage and 99 grass silage samples were selected with a broad range in chemical composition.
26331458	6	70	theme	grass	950:954	arg1	silages					956:962	grass silages	950:962	grass silages	950:962	The values for N fractions of maize and grass silages obtained with both methods were found different (p < 0.001).
26331458	4	71	theme	grass	569:573	arg1	samples					582:588	Ninety-nine maize silage and 99 grass silage samples	537:588	samples	582:588	Ninety-nine maize silage and 99 grass silage samples were selected with a broad range in chemical composition.
26331458	5	72	theme	silages	825:831	arg1	starch					809:814	starch	809:814	starch	809:814	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	1	73	theme	many	120:123	arg1	systems					141:147	many feed evaluation systems	120:147	many feed evaluation systems	120:147	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	7	74	theme	different	1276:1284	arg1	solution					1313:1320	water vs. buffer solution	1296:1320	solution	1313:1320	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	74	theme	different	1276:1284	arg1	solvents					1286:1293	different solvents	1276:1293	different solvents (water vs. buffer solution)	1276:1321	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	6	75	theme	maize	940:944	arg1	fractions					927:935	N fractions	925:935	N fractions of maize and grass silages obtained with both methods	925:989	The values for N fractions of maize and grass silages obtained with both methods were found different (p < 0.001).
26331458	0	76	theme	grass	73:77	arg1	silages					79:85	grass silages	73:85	grass silages	73:85	Comparison of fractionation methods for nitrogen and starch in maize and grass silages.
26331458	1	77	theme	evaluation	130:139	arg1	systems					141:147	many feed evaluation systems	120:147	many feed evaluation systems	120:147	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	4	78	theme	maize	549:553	arg1	silage					555:560	Ninety-nine maize silage and 99 grass silage samples	537:588	silage	555:560	Ninety-nine maize silage and 99 grass silage samples were selected with a broad range in chemical composition.
26331458	5	79	theme	chemical	888:895	arg1	composition					897:907	their chemical composition	882:907	their chemical composition	882:907	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	8	80	theme	rumen	1482:1486	arg1	degradability					1495:1507	the effective rumen starch degradability	1468:1507	the effective rumen starch degradability	1468:1507	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods can led to different predicted values for the effective rumen starch degradability.
26331458	0	81	from	starch	53:58	arg1	maize					63:67	maize	63:67	maize	63:67	Comparison of fractionation methods for nitrogen and starch in maize and grass silages.
26331458	0	81	from	starch	53:58	arg1	silages					79:85	grass silages	73:85	grass silages	73:85	Comparison of fractionation methods for nitrogen and starch in maize and grass silages.
26331458	9	82	theme	different	1696:1704	arg1	programs					1714:1721	different washing programs	1696:1721	different washing programs	1696:1721	In conclusion, the MM with one recommended shaking procedure, performed under identical and controlled experimental conditions, can give more reliable results compared to the WMM, using different washing programs and procedures.
26331458	0	83	from	nitrogen	40:47	arg1	maize					63:67	maize	63:67	maize	63:67	Comparison of fractionation methods for nitrogen and starch in maize and grass silages.
26331458	0	83	from	nitrogen	40:47	arg1	silages					79:85	grass silages	73:85	grass silages	73:85	Comparison of fractionation methods for nitrogen and starch in maize and grass silages.
26331458	2	84	theme	modified	318:325	arg1	MM					335:336	MM	335:336	MM	335:336	As this method has some disadvantages, an alternate modified method (MM) was recently introduced.
26331458	2	84	theme	modified	318:325	arg1	method					327:332	an alternate modified method	305:332	an alternate modified method (MM)	305:337	As this method has some disadvantages, an alternate modified method (MM) was recently introduced.
26331458	0	85	theme	methods	28:34	arg1	Comparison					0:9	Comparison	0:9	Comparison of fractionation methods for nitrogen and starch in maize and grass silages.	0:86	Comparison of fractionation methods for nitrogen and starch in maize and grass silages.
26331458	5	86	theme	silages	745:751	arg1	N					724:724	N	724:724	N of maize and grass silages	724:751	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	3	87	theme	study	380:384	arg1	aim					368:370	The aim	364:370	The aim of this study	364:384	The aim of this study was to determine and compare the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages, using the WMM and the MM.
26331458	3	88	theme	silages	502:508	arg1	fractions					443:451	the W and non-washout (D+U) fractions	415:451	the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages	415:508	The aim of this study was to determine and compare the W and non-washout (D+U) fractions of nitrogen (N) and/or starch of maize and grass silages, using the WMM and the MM.
26331458	5	89	from	variation	869:877	arg1	composition					897:907	their chemical composition	882:907	their chemical composition	882:907	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	1	90	theme	incubated	244:252	arg1	bags					260:263	the rumen incubated nylon bags	234:263	the rumen incubated nylon bags	234:263	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26331458	7	91	theme	rinsing	1244:1250	arg1	solution					1313:1320	water vs. buffer solution	1296:1320	solution	1313:1320	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	91	theme	rinsing	1244:1250	arg1	solvents					1286:1293	different solvents	1276:1293	different solvents (water vs. buffer solution)	1276:1321	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	91	theme	rinsing	1244:1250	arg1	duration					1232:1239	duration	1232:1239	duration of rinsing (40 min vs. 60 min)	1232:1270	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	91	theme	rinsing	1244:1250	arg1	procedures					1198:1207	different rinsing procedures	1180:1207	different rinsing procedures (washing vs. shaking)	1180:1229	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	5	92	dep	soluble	691:697	arg1	S					700:700	S	700:700	S	700:700	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	5	93	theme	washout	774:780	arg1	W-S					783:785	the W, insoluble washout (W-S) and D+U fractions	757:804	W-S	783:785	The results showed a large range in the W, soluble (S) and D+U fractions of N of maize and grass silages and the W, insoluble washout (W-S) and D+U fractions of starch of maize silages, determined by both methods, due to variation in their chemical composition.
26331458	4	94	theme	chemical	626:633	arg1	composition					635:645	chemical composition	626:645	chemical composition	626:645	Ninety-nine maize silage and 99 grass silage samples were selected with a broad range in chemical composition.
26331458	7	95	dep	rinsing	1244:1250	arg1	60 min					1264:1269	60 min	1264:1269	60 min	1264:1269	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	7	95	dep	rinsing	1244:1250	arg1	40 min					1253:1258	40 min	1253:1258	40 min	1253:1258	Large differences (p < 0.001) were found in the D+U fraction of starch of maize silages which might be due to different methodological approaches, such as different rinsing procedures (washing vs. shaking), duration of rinsing (40 min vs. 60 min) and different solvents (water vs. buffer solution).
26331458	9	96	theme	recommended	1541:1551	arg1	procedure					1561:1569	one recommended shaking procedure	1537:1569	one recommended shaking procedure	1537:1569	In conclusion, the MM with one recommended shaking procedure, performed under identical and controlled experimental conditions, can give more reliable results compared to the WMM, using different washing programs and procedures.
26331458	8	97	theme	W-S	1365:1367	arg1	fractions					1377:1385	the W-S and D+U fractions	1361:1385	the W-S and D+U fractions of starch determined with both methods	1361:1424	The large differences (p < 0.001) in the W-S and D+U fractions of starch determined with both methods can led to different predicted values for the effective rumen starch degradability.
26331458	1	98	theme	washout	201:207	arg1	fraction					213:220	the washout (W) fraction	197:220	the washout (W) fraction	197:220	In in situ nylon bag technique, many feed evaluation systems use a washing machine method (WMM) to determine the washout (W) fraction and to wash the rumen incubated nylon bags.
26452886	0	0	theme	performance	96:106	arg1	bioelectrode					63:74	composite based bioelectrode	47:74	composite based bioelectrode of improved sensing performance	47:106	Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance.
26452886	4	1	dep	electrode	884:892	arg1	contrast					862:869	contrast	862:869	contrast	862:869	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	4	2	from	evident	722:728	arg1	electrode					884:892	CH-GO/ITO electrode	874:892	CH-GO/ITO electrode (6×10(-3) cm/s)	874:908	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	4	2	from	evident	722:728	arg1	cm/s					904:907	6×10(-3) cm/s	895:907	6×10(-3) cm/s	895:907	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	0	3	theme	sensing	88:94	arg1	performance					96:106	improved sensing performance	79:106	improved sensing performance	79:106	Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance.
26452886	4	4	from	electrode	884:892	arg1	evident					722:728	evident	722:728	evident	722:728	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	5	5	theme	enzyme	1000:1005	arg1	affinity					988:995	binding affinity	980:995	binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences	980:1112	Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences.
26452886	4	6	from	value	789:793	arg1	evident					722:728	evident	722:728	evident	722:728	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	6	7	theme	better	1127:1132	arg1	capacity					1142:1149	better loading capacity	1127:1149	better loading capacity	1127:1149	Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.
26452886	4	8	theme	constant	775:782	arg1	value					789:793	one order higher electron transfer rate constant (Ks) value	735:793	one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s)	735:856	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	4	9	theme	rate	770:773	arg1	constant					775:782	electron transfer rate constant	752:782	one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s)	735:856	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	4	9	theme	rate	770:773	arg1	Ks					785:786	Ks	785:786	Ks	785:786	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	6	10	theme	tremendous	1219:1228	arg1	potential					1230:1238	tremendous potential	1219:1238	tremendous potential to detect several bio-analytes in the coming future	1219:1290	Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.
26452886	5	11	theme	low	930:932	arg1	mM					960:961	0.85 mM	955:961	0.85 mM	955:961	Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences.
26452886	5	11	theme	low	930:932	arg1	value					937:941	very low Km value	925:941	very low Km value [15.4 mg/dL(0.85 mM)]	925:963	Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences.
26452886	4	12	theme	higher	745:750	arg1	value					789:793	one order higher electron transfer rate constant (Ks) value	735:793	one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s)	735:856	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	5	13	theme	Km	934:935	arg1	mM					960:961	0.85 mM	955:961	0.85 mM	955:961	Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences.
26452886	5	13	theme	Km	934:935	arg1	value					937:941	very low Km value	925:941	very low Km value [15.4 mg/dL(0.85 mM)]	925:963	Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences.
26452886	2	14	theme	range	476:480	arg1	sensitivity					429:439	high sensitivity	424:439	high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL	424:496	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	0	15	theme	composite	47:55	arg1	bioelectrode					63:74	composite based bioelectrode	47:74	composite based bioelectrode of improved sensing performance	47:106	Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance.
26452886	2	16	theme	ITO	300:302	arg1	substrate					304:312	ITO substrate	300:312	ITO substrate	300:312	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	2	17	theme	µA/	448:450	arg1	sensitivity					429:439	high sensitivity	424:439	high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL	424:496	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	0	18	theme	reduced	10:16	arg1	oxide-gold					27:36	Partially reduced graphene oxide-gold	0:36	Partially reduced graphene oxide-gold	0:36	Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance.
26452886	5	19	theme	good	1043:1046	arg1	stability					1058:1066	good biosensor stability	1043:1066	good biosensor stability	1043:1066	Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences.
26452886	6	20	theme	CH-prGO-AuNRs	1189:1201	arg1	composite					1203:1211	the proposed CH-prGO-AuNRs composite	1176:1211	the proposed CH-prGO-AuNRs composite	1176:1211	Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.
26452886	2	21	theme	CH-prGO-AuNRs	349:361	arg1	composite					363:371	CH-prGO-AuNRs composite	349:371	CH-prGO-AuNRs composite	349:371	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	6	22	theme	proposed	1180:1187	arg1	composite					1203:1211	the proposed CH-prGO-AuNRs composite	1176:1211	the proposed CH-prGO-AuNRs composite	1176:1211	Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.
26452886	3	23	theme	based	579:583	arg1	electrodes					585:594	these CH-prGO-AuNRs/ITO based electrodes	555:594	these CH-prGO-AuNRs/ITO based electrodes	555:594	Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode.
26452886	4	24	theme	transfer	761:768	arg1	constant					775:782	electron transfer rate constant	752:782	one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s)	735:856	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	4	24	theme	transfer	761:768	arg1	Ks					785:786	Ks	785:786	Ks	785:786	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	4	25	theme	modified	820:827	arg1	cm/s					852:855	12.4×10(-2) cm/s	840:855	12.4×10(-2) cm/s	840:855	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	4	25	theme	modified	820:827	arg1	electrode					829:837	CH-prGO-AuNRs modified electrode	806:837	CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s)	806:856	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	1	26	theme	present	113:119	arg1	work					121:124	The present work	109:124	The present work	109:124	The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing.
26452886	0	27	theme	graphene	18:25	arg1	oxide-gold					27:36	Partially reduced graphene oxide-gold	0:36	Partially reduced graphene oxide-gold	0:36	Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance.
26452886	3	28	theme	graphene	672:679	arg1	electrode					703:711	simple graphene oxide based CH-GO/ITO electrode	665:711	simple graphene oxide based CH-GO/ITO electrode	665:711	Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode.
26452886	2	29	theme	glucose	330:336	arg1	oxidase					338:344	glucose oxidase	330:344	glucose oxidase	330:344	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	2	30	theme	mg/dL	492:496	arg1	µA/					448:450	3.2 µA/	444:450	3.2 µA/(mg/dL)/cm(2)	444:463	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	2	30	theme	mg/dL	492:496	arg1	mg/dL					452:456	mg/dL	452:456	mg/dL	452:456	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	2	30	theme	mg/dL	492:496	arg1	range					476:480	linear range	469:480	linear range of 25-200 mg/dL	469:496	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	3	31	theme	oxide	681:685	arg1	electrode					703:711	simple graphene oxide based CH-GO/ITO electrode	665:711	simple graphene oxide based CH-GO/ITO electrode	665:711	Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode.
26452886	6	32	theme	loading	1134:1140	arg1	capacity					1142:1149	better loading capacity	1127:1149	better loading capacity	1127:1149	Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.
26452886	1	33	theme	potential	225:233	arg1	material					248:255	a potential bioelectrode material	223:255	a potential bioelectrode material for enhanced glucose sensing	223:284	The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing.
26452886	3	34	theme	enhanced	620:627	arg1	properties					637:646	synergistiacally enhanced sensing properties	603:646	synergistiacally enhanced sensing properties	603:646	Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode.
26452886	1	35	theme	bioelectrode	235:246	arg1	material					248:255	a potential bioelectrode material	223:255	a potential bioelectrode material for enhanced glucose sensing	223:284	The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing.
26452886	5	36	theme	environmental	1086:1098	arg1	interferences					1100:1112	environmental interferences	1086:1112	environmental interferences	1086:1112	Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences.
26452886	3	37	theme	simple	665:670	arg1	electrode					703:711	simple graphene oxide based CH-GO/ITO electrode	665:711	simple graphene oxide based CH-GO/ITO electrode	665:711	Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode.
26452886	2	38	theme	CH-prGO-AuNRs/ITO	380:396	arg1	bioelectrodes					398:410	these CH-prGO-AuNRs/ITO bioelectrodes	374:410	these CH-prGO-AuNRs/ITO bioelectrodes	374:410	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	0	39	theme	based	57:61	arg1	bioelectrode					63:74	composite based bioelectrode	47:74	composite based bioelectrode of improved sensing performance	47:106	Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance.
26452886	4	40	theme	CH-GO/ITO	874:882	arg1	electrode					884:892	CH-GO/ITO electrode	874:892	CH-GO/ITO electrode (6×10(-3) cm/s)	874:908	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	4	40	theme	CH-GO/ITO	874:882	arg1	cm/s					904:907	6×10(-3) cm/s	895:907	6×10(-3) cm/s	895:907	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	5	41	dep	value	937:941	arg1	mg/dL					949:953	[15.4 mg/dL	943:953	very low Km value [15.4 mg/dL(0.85 mM)]	925:963	Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences.
26452886	4	42	theme	CH-prGO-AuNRs	806:818	arg1	cm/s					852:855	12.4×10(-2) cm/s	840:855	12.4×10(-2) cm/s	840:855	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	4	42	theme	CH-prGO-AuNRs	806:818	arg1	electrode					829:837	CH-prGO-AuNRs modified electrode	806:837	CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s)	806:856	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	5	43	theme	binding	980:986	arg1	affinity					988:995	binding affinity	980:995	binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences	980:1112	Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences.
26452886	1	44	theme	reduced	145:151	arg1	nanorods					173:180	partially reduced graphene oxide-gold nanorods	135:180	partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing	135:284	The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing.
26452886	6	45	theme	several	1250:1256	arg1	bio-analytes					1258:1269	several bio-analytes	1250:1269	several bio-analytes	1250:1269	Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.
26452886	3	46	theme	based	687:691	arg1	electrode					703:711	simple graphene oxide based CH-GO/ITO electrode	665:711	simple graphene oxide based CH-GO/ITO electrode	665:711	Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode.
26452886	1	47	theme	graphene	153:160	arg1	nanorods					173:180	partially reduced graphene oxide-gold nanorods	135:180	partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing	135:284	The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing.
26452886	4	48	theme	electron	752:759	arg1	constant					775:782	electron transfer rate constant	752:782	one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s)	735:856	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	4	48	theme	electron	752:759	arg1	Ks					785:786	Ks	785:786	Ks	785:786	This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10(-2) cm/s), in contrast to CH-GO/ITO electrode (6×10(-3) cm/s).
26452886	0	49	theme	improved	79:86	arg1	performance					96:106	improved sensing performance	79:106	improved sensing performance	79:106	Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance.
26452886	3	50	theme	CH-GO/ITO	693:701	arg1	electrode					703:711	simple graphene oxide based CH-GO/ITO electrode	665:711	simple graphene oxide based CH-GO/ITO electrode	665:711	Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode.
26452886	3	51	theme	sensing	629:635	arg1	properties					637:646	synergistiacally enhanced sensing properties	603:646	synergistiacally enhanced sensing properties	603:646	Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode.
26452886	1	52	theme	oxide-gold	162:171	arg1	nanorods					173:180	partially reduced graphene oxide-gold nanorods	135:180	partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing	135:284	The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing.
26452886	1	53	theme	enhanced	261:268	arg1	sensing					278:284	enhanced glucose sensing	261:284	enhanced glucose sensing	261:284	The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing.
26452886	2	54	theme	linear	469:474	arg1	range					476:480	linear range	469:480	linear range of 25-200 mg/dL	469:496	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	5	55	theme	biosensor	1048:1056	arg1	stability					1058:1066	good biosensor stability	1043:1066	good biosensor stability	1043:1066	Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences.
26452886	2	56	dep	µA/	448:450	arg1	/cm					458:460	/cm(2)	458:463	3.2 µA/(mg/dL)/cm(2)	444:463	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	2	57	theme	high	424:427	arg1	sensitivity					429:439	high sensitivity	424:439	high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL	424:496	Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 µA/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL.
26452886	1	58	theme	glucose	270:276	arg1	sensing					278:284	enhanced glucose sensing	261:284	enhanced glucose sensing	261:284	The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing.
25762620	4	0	theme	breast	517:522	arg1	cancer					524:529	breast cancer	517:529	breast cancer	517:529	This study aims to determine the correlation between C1GALT1 expression and breast cancer clinicopathological features and roles of C1GALT1 in breast cancer malignant phenotypes.
25762620	5	1	theme	C1GALT1	679:685	arg1	protein					687:693	C1GALT1 protein	679:693	C1GALT1 protein	679:693	Public databases and our data showed that C1GALT1 mRNA and C1GALT1 protein are frequently up-regulated in breast cancer; and increased C1GALT1 expression correlates with higher histological grade and advanced tumor stage.
25762620	9	2	theme	cancer	1223:1228	arg1	progression					1240:1250	breast cancer malignant progression	1216:1250	breast cancer malignant progression	1216:1250	These findings suggest that C1GALT1 enhances breast cancer malignant progression through promoting MUC1-C/β-catenin signaling pathway.
25762620	5	3	theme	higher	790:795	arg1	grade					810:814	higher histological grade	790:814	higher histological grade	790:814	Public databases and our data showed that C1GALT1 mRNA and C1GALT1 protein are frequently up-regulated in breast cancer; and increased C1GALT1 expression correlates with higher histological grade and advanced tumor stage.
25762620	4	4	dep	correlation	474:484	arg1	phenotypes					608:617	malignant phenotypes	598:617	malignant phenotypes	598:617	This study aims to determine the correlation between C1GALT1 expression and breast cancer clinicopathological features and roles of C1GALT1 in breast cancer malignant phenotypes.
25762620	0	5	theme	signaling	75:83	arg1	pathway					85:91	MUC1-C signaling pathway	68:91	MUC1-C signaling pathway	68:91	Up-regulation of C1GALT1 promotes breast cancer cell growth through MUC1-C signaling pathway.
25762620	5	6	theme	tumor	829:833	arg1	stage					835:839	advanced tumor stage	820:839	advanced tumor stage	820:839	Public databases and our data showed that C1GALT1 mRNA and C1GALT1 protein are frequently up-regulated in breast cancer; and increased C1GALT1 expression correlates with higher histological grade and advanced tumor stage.
25762620	6	7	theme	cell	891:894	arg1	growth					896:901	breast cancer cell growth	877:901	breast cancer cell growth	877:901	Overexpression of C1GALT1 enhanced breast cancer cell growth, migration, and invasion in vitro as well as tumor growth in vivo.
25762620	6	8	theme	breast	877:882	arg1	growth					896:901	breast cancer cell growth	877:901	breast cancer cell growth	877:901	Overexpression of C1GALT1 enhanced breast cancer cell growth, migration, and invasion in vitro as well as tumor growth in vivo.
25762620	10	9	theme	agent	1507:1511	arg1	development					1513:1523	novel therapeutic agent development	1489:1523	novel therapeutic agent development	1489:1523	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	4	10	theme	clinicopathological	531:549	arg1	features					551:558	clinicopathological features	531:558	clinicopathological features	531:558	This study aims to determine the correlation between C1GALT1 expression and breast cancer clinicopathological features and roles of C1GALT1 in breast cancer malignant phenotypes.
25762620	10	11	theme	development	1513:1523	arg1	target					1479:1484	a target	1477:1484	a target of novel therapeutic agent development	1477:1523	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	7	12	theme	C1GALT1	982:988	arg1	knockdown					990:998	C1GALT1 knockdown	982:998	C1GALT1 knockdown	982:998	Conversely, C1GALT1 knockdown suppressed these malignant phenotypes.
25762620	10	13	theme	C1GALT1	1332:1338	arg1	function					1320:1327	the function	1316:1327	the function of C1GALT1 in breast cancer	1316:1355	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	4	14	theme	C1GALT1	494:500	arg1	expression					502:511	C1GALT1 expression	494:511	C1GALT1 expression	494:511	This study aims to determine the correlation between C1GALT1 expression and breast cancer clinicopathological features and roles of C1GALT1 in breast cancer malignant phenotypes.
25762620	8	15	theme	Mucin	1093:1097	arg1	MUC					1100:1102	Mucin (MUC) 1	1093:1105	Mucin (MUC) 1	1093:1105	Furthermore, C1GALT1 modulates O-glycan structures on Mucin (MUC) 1 and promotes MUC1-C/β-catenin signaling in breast cancer cells.
25762620	6	16	theme	C1GALT1	860:866	arg1	Overexpression					842:855	Overexpression	842:855	Overexpression of C1GALT1	842:866	Overexpression of C1GALT1 enhanced breast cancer cell growth, migration, and invasion in vitro as well as tumor growth in vivo.
25762620	10	17	theme	breast	1343:1348	arg1	cancer					1350:1355	breast cancer	1343:1355	breast cancer	1343:1355	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	2	18	theme	structure	280:288	arg1	biosynthesis					248:259	the biosynthesis	244:259	the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis	244:374	Core 1 β1,3-galactosyltransferase (C1GALT1) is an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis.
25762620	1	19	gly	glycosylation	103:115	arg1	cancers					143:149	cancers	143:149	cancers	143:149	Aberrant glycosylation is frequently observed in cancers.
25762620	2	20	theme	Core	152:155	arg1	enzyme					212:217	an exclusive enzyme	199:217	an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis	199:374	Core 1 β1,3-galactosyltransferase (C1GALT1) is an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis.
25762620	2	20	theme	Core	152:155	arg1	C1GALT1					187:193	C1GALT1	187:193	C1GALT1	187:193	Core 1 β1,3-galactosyltransferase (C1GALT1) is an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis.
25762620	2	20	theme	Core	152:155	arg1	β1,3-galactosyltransferase					159:184	Core 1 β1,3-galactosyltransferase	152:184	Core 1 β1,3-galactosyltransferase (C1GALT1)	152:194	Core 1 β1,3-galactosyltransferase (C1GALT1) is an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis.
25762620	0	21	theme	cancer	41:46	arg1	growth					53:58	breast cancer cell growth	34:58	breast cancer cell growth	34:58	Up-regulation of C1GALT1 promotes breast cancer cell growth through MUC1-C signaling pathway.
25762620	9	22	theme	MUC1-C/β-catenin	1270:1285	arg1	pathway					1297:1303	MUC1-C/β-catenin signaling pathway	1270:1303	MUC1-C/β-catenin signaling pathway	1270:1303	These findings suggest that C1GALT1 enhances breast cancer malignant progression through promoting MUC1-C/β-catenin signaling pathway.
25762620	2	23	theme	O-glycan	271:278	arg1	Gal-GalNAc-O-Ser/Thr					291:310	Gal-GalNAc-O-Ser/Thr	291:310	Gal-GalNAc-O-Ser/Thr	291:310	Core 1 β1,3-galactosyltransferase (C1GALT1) is an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis.
25762620	2	23	theme	O-glycan	271:278	arg1	structure					280:288	core 1 O-glycan structure	264:288	core 1 O-glycan structure	264:288	Core 1 β1,3-galactosyltransferase (C1GALT1) is an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis.
25762620	10	24	theme	new	1363:1365	arg1	insights					1367:1374	new insights	1363:1374	new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis	1363:1435	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	5	25	theme	advanced	820:827	arg1	stage					835:839	advanced tumor stage	820:839	advanced tumor stage	820:839	Public databases and our data showed that C1GALT1 mRNA and C1GALT1 protein are frequently up-regulated in breast cancer; and increased C1GALT1 expression correlates with higher histological grade and advanced tumor stage.
25762620	5	26	theme	histological	797:808	arg1	grade					810:814	higher histological grade	790:814	higher histological grade	790:814	Public databases and our data showed that C1GALT1 mRNA and C1GALT1 protein are frequently up-regulated in breast cancer; and increased C1GALT1 expression correlates with higher histological grade and advanced tumor stage.
25762620	8	27	theme	breast	1150:1155	arg1	cells					1164:1168	breast cancer cells	1150:1168	breast cancer cells	1150:1168	Furthermore, C1GALT1 modulates O-glycan structures on Mucin (MUC) 1 and promotes MUC1-C/β-catenin signaling in breast cancer cells.
25762620	6	28	theme	tumor	948:952	arg1	growth					954:959	tumor growth	948:959	breast cancer cell growth, migration, and invasion in vitro as well as tumor growth	877:959	Overexpression of C1GALT1 enhanced breast cancer cell growth, migration, and invasion in vitro as well as tumor growth in vivo.
25762620	4	29	theme	breast	584:589	arg1	cancer					591:596	breast cancer	584:596	breast cancer	584:596	This study aims to determine the correlation between C1GALT1 expression and breast cancer clinicopathological features and roles of C1GALT1 in breast cancer malignant phenotypes.
25762620	5	30	theme	Public	620:625	arg1	databases					627:635	Public databases	620:635	Public databases	620:635	Public databases and our data showed that C1GALT1 mRNA and C1GALT1 protein are frequently up-regulated in breast cancer; and increased C1GALT1 expression correlates with higher histological grade and advanced tumor stage.
25762620	1	31	theme	Aberrant	94:101	arg1	glycosylation					103:115	Aberrant glycosylation	94:115	Aberrant glycosylation	94:115	Aberrant glycosylation is frequently observed in cancers.
25762620	10	32	theme	C1GALT1	1392:1398	arg1	roles					1383:1387	the roles	1379:1387	the roles of C1GALT1 and O-glycosylation in tumorigenesis	1379:1435	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	8	33	theme	O-glycan	1070:1077	arg1	structures					1079:1088	O-glycan structures	1070:1088	O-glycan structures on Mucin (MUC) 1	1070:1105	Furthermore, C1GALT1 modulates O-glycan structures on Mucin (MUC) 1 and promotes MUC1-C/β-catenin signaling in breast cancer cells.
25762620	4	34	dep	expression	502:511	arg1	features					551:558	clinicopathological features	531:558	clinicopathological features	531:558	This study aims to determine the correlation between C1GALT1 expression and breast cancer clinicopathological features and roles of C1GALT1 in breast cancer malignant phenotypes.
25762620	4	35	from	correlation	474:484	arg1	cancer					591:596	breast cancer	584:596	breast cancer	584:596	This study aims to determine the correlation between C1GALT1 expression and breast cancer clinicopathological features and roles of C1GALT1 in breast cancer malignant phenotypes.
25762620	9	36	theme	malignant	1230:1238	arg1	progression					1240:1250	breast cancer malignant progression	1216:1250	breast cancer malignant progression	1216:1250	These findings suggest that C1GALT1 enhances breast cancer malignant progression through promoting MUC1-C/β-catenin signaling pathway.
25762620	0	37	theme	C1GALT1	17:23	arg1	Up-regulation					0:12	Up-regulation	0:12	Up-regulation of C1GALT1	0:23	Up-regulation of C1GALT1 promotes breast cancer cell growth through MUC1-C signaling pathway.
25762620	4	38	theme	malignant	598:606	arg1	phenotypes					608:617	malignant phenotypes	598:617	malignant phenotypes	598:617	This study aims to determine the correlation between C1GALT1 expression and breast cancer clinicopathological features and roles of C1GALT1 in breast cancer malignant phenotypes.
25762620	10	39	theme	novel	1489:1493	arg1	development					1513:1523	novel therapeutic agent development	1489:1523	novel therapeutic agent development	1489:1523	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	3	40	theme	breast	426:431	arg1	cancer					433:438	breast cancer	426:438	breast cancer	426:438	Little is known about the function of C1GALT1 in breast cancer.
25762620	8	41	from	structures	1079:1088	arg1	MUC					1100:1102	Mucin (MUC) 1	1093:1105	Mucin (MUC) 1	1093:1105	Furthermore, C1GALT1 modulates O-glycan structures on Mucin (MUC) 1 and promotes MUC1-C/β-catenin signaling in breast cancer cells.
25762620	5	42	theme	increased	745:753	arg1	expression					763:772	increased C1GALT1 expression	745:772	increased C1GALT1 expression	745:772	Public databases and our data showed that C1GALT1 mRNA and C1GALT1 protein are frequently up-regulated in breast cancer; and increased C1GALT1 expression correlates with higher histological grade and advanced tumor stage.
25762620	0	43	theme	breast	34:39	arg1	growth					53:58	breast cancer cell growth	34:58	breast cancer cell growth	34:58	Up-regulation of C1GALT1 promotes breast cancer cell growth through MUC1-C signaling pathway.
25762620	5	44	theme	C1GALT1	755:761	arg1	expression					763:772	increased C1GALT1 expression	745:772	increased C1GALT1 expression	745:772	Public databases and our data showed that C1GALT1 mRNA and C1GALT1 protein are frequently up-regulated in breast cancer; and increased C1GALT1 expression correlates with higher histological grade and advanced tumor stage.
25762620	2	45	from	enzyme	212:217	arg1	humans					222:227	humans	222:227	humans	222:227	Core 1 β1,3-galactosyltransferase (C1GALT1) is an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis.
25762620	3	46	theme	C1GALT1	415:421	arg1	function					403:410	the function	399:410	the function of C1GALT1 in breast cancer	399:438	Little is known about the function of C1GALT1 in breast cancer.
25762620	8	47	theme	cancer	1157:1162	arg1	cells					1164:1168	breast cancer cells	1150:1168	breast cancer cells	1150:1168	Furthermore, C1GALT1 modulates O-glycan structures on Mucin (MUC) 1 and promotes MUC1-C/β-catenin signaling in breast cancer cells.
25762620	3	48	from	function	403:410	arg1	cancer					433:438	breast cancer	426:438	breast cancer	426:438	Little is known about the function of C1GALT1 in breast cancer.
25762620	0	49	theme	cell	48:51	arg1	growth					53:58	breast cancer cell growth	34:58	breast cancer cell growth	34:58	Up-regulation of C1GALT1 promotes breast cancer cell growth through MUC1-C signaling pathway.
25762620	4	50	theme	C1GALT1	573:579	arg1	roles					564:568	roles	564:568	roles of C1GALT1 in breast cancer	564:596	This study aims to determine the correlation between C1GALT1 expression and breast cancer clinicopathological features and roles of C1GALT1 in breast cancer malignant phenotypes.
25762620	4	50	theme	C1GALT1	573:579	arg1	correlation					474:484	the correlation	470:484	the correlation between C1GALT1 expression and breast cancer clinicopathological features	470:558	This study aims to determine the correlation between C1GALT1 expression and breast cancer clinicopathological features and roles of C1GALT1 in breast cancer malignant phenotypes.
25762620	8	51	theme	MUC1-C/β-catenin	1120:1135	arg1	signaling					1137:1145	MUC1-C/β-catenin signaling	1120:1145	MUC1-C/β-catenin signaling in breast cancer cells	1120:1168	Furthermore, C1GALT1 modulates O-glycan structures on Mucin (MUC) 1 and promotes MUC1-C/β-catenin signaling in breast cancer cells.
25762620	5	52	theme	C1GALT1	662:668	arg1	mRNA					670:673	C1GALT1 mRNA	662:673	C1GALT1 mRNA	662:673	Public databases and our data showed that C1GALT1 mRNA and C1GALT1 protein are frequently up-regulated in breast cancer; and increased C1GALT1 expression correlates with higher histological grade and advanced tumor stage.
25762620	10	53	theme	O-glycosylation	1404:1418	arg1	roles					1383:1387	the roles	1379:1387	the roles of C1GALT1 and O-glycosylation in tumorigenesis	1379:1435	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	5	54	theme	breast	726:731	arg1	cancer					733:738	breast cancer	726:738	breast cancer	726:738	Public databases and our data showed that C1GALT1 mRNA and C1GALT1 protein are frequently up-regulated in breast cancer; and increased C1GALT1 expression correlates with higher histological grade and advanced tumor stage.
25762620	10	55	from	roles	1383:1387	arg1	tumorigenesis					1423:1435	tumorigenesis	1423:1435	tumorigenesis	1423:1435	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	9	56	theme	breast	1216:1221	arg1	cancer					1223:1228	breast cancer	1216:1228	breast cancer malignant progression	1216:1250	These findings suggest that C1GALT1 enhances breast cancer malignant progression through promoting MUC1-C/β-catenin signaling pathway.
25762620	10	57	from	function	1320:1327	arg1	cancer					1350:1355	breast cancer	1343:1355	breast cancer	1343:1355	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	2	58	theme	exclusive	202:210	arg1	enzyme					212:217	an exclusive enzyme	199:217	an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis	199:374	Core 1 β1,3-galactosyltransferase (C1GALT1) is an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis.
25762620	2	58	theme	exclusive	202:210	arg1	β1,3-galactosyltransferase					159:184	Core 1 β1,3-galactosyltransferase	152:184	Core 1 β1,3-galactosyltransferase (C1GALT1)	152:194	Core 1 β1,3-galactosyltransferase (C1GALT1) is an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis.
25762620	2	59	theme	core	264:267	arg1	Gal-GalNAc-O-Ser/Thr					291:310	Gal-GalNAc-O-Ser/Thr	291:310	Gal-GalNAc-O-Ser/Thr	291:310	Core 1 β1,3-galactosyltransferase (C1GALT1) is an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis.
25762620	2	59	theme	core	264:267	arg1	structure					280:288	core 1 O-glycan structure	264:288	core 1 O-glycan structure	264:288	Core 1 β1,3-galactosyltransferase (C1GALT1) is an exclusive enzyme in humans that catalyzes the biosynthesis of core 1 O-glycan structure, Gal-GalNAc-O-Ser/Thr, whose expression is commonly up-regulated during tumorigenesis.
25762620	8	60	from	signaling	1137:1145	arg1	cells					1164:1168	breast cancer cells	1150:1168	breast cancer cells	1150:1168	Furthermore, C1GALT1 modulates O-glycan structures on Mucin (MUC) 1 and promotes MUC1-C/β-catenin signaling in breast cancer cells.
25762620	0	61	theme	MUC1-C	68:73	arg1	pathway					85:91	MUC1-C signaling pathway	68:91	MUC1-C signaling pathway	68:91	Up-regulation of C1GALT1 promotes breast cancer cell growth through MUC1-C signaling pathway.
25762620	6	62	theme	cancer	884:889	arg1	growth					896:901	breast cancer cell growth	877:901	breast cancer cell growth	877:901	Overexpression of C1GALT1 enhanced breast cancer cell growth, migration, and invasion in vitro as well as tumor growth in vivo.
25762620	10	63	theme	C1GALT1	1466:1472	arg1	potential					1453:1461	the potential	1449:1461	the potential of C1GALT1 as a target of novel therapeutic agent development	1449:1523	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	10	64	dep	novel	1489:1493	arg1	therapeutic					1495:1505	therapeutic	1495:1505	therapeutic	1495:1505	Unveiling the function of C1GALT1 in breast cancer opens new insights to the roles of C1GALT1 and O-glycosylation in tumorigenesis and renders the potential of C1GALT1 as a target of novel therapeutic agent development.
25762620	9	65	theme	signaling	1287:1295	arg1	pathway					1297:1303	MUC1-C/β-catenin signaling pathway	1270:1303	MUC1-C/β-catenin signaling pathway	1270:1303	These findings suggest that C1GALT1 enhances breast cancer malignant progression through promoting MUC1-C/β-catenin signaling pathway.
25762620	4	66	from	roles	564:568	arg1	cancer					591:596	breast cancer	584:596	breast cancer	584:596	This study aims to determine the correlation between C1GALT1 expression and breast cancer clinicopathological features and roles of C1GALT1 in breast cancer malignant phenotypes.
25762620	1	67	located	observed	131:138	arg1	cancers					143:149	cancers	143:149	cancers	143:149	Aberrant glycosylation is frequently observed in cancers.
25762620	1	67	located	observed	131:138	arg2	glycosylation					103:115	Aberrant glycosylation	94:115	Aberrant glycosylation	94:115	Aberrant glycosylation is frequently observed in cancers.
25762620	7	68	theme	malignant	1017:1025	arg1	phenotypes					1027:1036	these malignant phenotypes	1011:1036	these malignant phenotypes	1011:1036	Conversely, C1GALT1 knockdown suppressed these malignant phenotypes.
29054519	0	0	theme	sativa	190:195	arg1	cv					200:201	Lactuca sativa L. cv	182:201	Lactuca sativa L. cv	182:201	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	2	1	theme	%	377:377	arg1	H2SO4					379:383	64wt% H2SO4	373:383	64wt% H2SO4 at 55°C	373:391	The isolated cellulose was hydrolyzed using 64wt% H2SO4 at 55°C under constant stirring for 1h to obtain cellulose nanocrystals (CNCs).
29054519	6	2	from	property	1152:1159	arg1	porosity					1114:1121	porosity	1114:1121	porosity	1114:1121	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	6	2	from	property	1152:1159	arg1	concentration					1073:1085	the concentration	1069:1085	the concentration of CNCs added	1069:1099	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	6	3	theme	water	977:981	arg1	chitosan					929:936	chitosan	929:936	chitosan	929:936	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	6	3	theme	water	977:981	arg1	acid					949:952	lactic acid	942:952	lactic acid	942:952	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	6	3	theme	water	977:981	arg1	removal					966:972	further removal	958:972	further removal of water	958:981	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	8	4	theme	certain	1492:1498	arg1	applications					1519:1530	certain tissue engineering applications	1492:1530	certain tissue engineering applications	1492:1530	Moreover, these characteristics can give new properties that are necessary for certain tissue engineering applications.
29054519	0	5	theme	Lactuca	182:188	arg1	cv					200:201	Lactuca sativa L. cv	182:201	Lactuca sativa L. cv	182:201	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	6	dep	chitosan-g-d	123:134	arg1	leaf					209:212	green leaf	203:212	green leaf	203:212	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	6	7	from	viability	1170:1178	arg1	porosity					1114:1121	porosity	1114:1121	porosity	1114:1121	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	6	7	from	viability	1170:1178	arg1	concentration					1073:1085	the concentration	1069:1085	the concentration of CNCs added	1069:1099	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	0	8	theme	composite	90:98	arg1	scaffold					100:107	a composite scaffold	88:107	a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf	88:212	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	6	9	theme	cell	1165:1168	arg1	viability					1170:1178	cell viability	1165:1178	cell viability	1165:1178	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	6	10	theme	release	1144:1150	arg1	property					1152:1159	drug release property	1139:1159	drug release property	1139:1159	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	1	11	theme	bleaching	271:279	arg1	treatments					281:290	bleaching treatments	271:290	bleaching treatments	271:290	Through exhaustive extraction via successive alkali and bleaching treatments cellulose was isolated from lettuce.
29054519	8	12	theme	engineering	1507:1517	arg1	applications					1519:1530	certain tissue engineering applications	1492:1530	certain tissue engineering applications	1492:1530	Moreover, these characteristics can give new properties that are necessary for certain tissue engineering applications.
29054519	0	13	theme	L.	197:198	arg1	cv					200:201	Lactuca sativa L. cv	182:201	Lactuca sativa L. cv	182:201	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	14	link	derived	7:13	arg1	scaffolds					15:23	Nature derived scaffolds	0:23	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.	0:213	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	14	link	derived	7:13	arg1	fabrication					73:83	fabrication	73:83	fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf	73:212	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	14	link	derived	7:13	arg1	Design					62:67	Design	62:67	Design	62:67	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	4	15	theme	237±26	685:690	arg1	dimensions					671:680	mean dimensions	666:680	mean dimensions of 237±26, 33±12 and 32±7nm	666:708	The isolated CNCs have mean dimensions of 237±26, 33±12 and 32±7nm in length, thickness and height, respectively.
29054519	6	16	theme	further	958:964	arg1	removal					966:972	further removal	958:972	further removal of water	958:981	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	8	17	theme	tissue	1500:1505	arg1	applications					1519:1530	certain tissue engineering applications	1492:1530	certain tissue engineering applications	1492:1530	Moreover, these characteristics can give new properties that are necessary for certain tissue engineering applications.
29054519	6	18	dep	80°C.	1030:1034	arg1	show					1044:1047	show	1044:1047	show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds	1044:1232	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	6	19	from	degradability	1124:1136	arg1	porosity					1114:1121	porosity	1114:1121	porosity	1114:1121	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	6	19	from	degradability	1124:1136	arg1	concentration					1073:1085	the concentration	1069:1085	the concentration of CNCs added	1069:1099	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	4	20	theme	isolated	647:654	arg1	CNCs					656:659	The isolated CNCs	643:659	The isolated CNCs	643:659	The isolated CNCs have mean dimensions of 237±26, 33±12 and 32±7nm in length, thickness and height, respectively.
29054519	0	21	theme	scaffold	100:107	arg1	fabrication					73:83	fabrication	73:83	fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf	73:212	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	21	theme	scaffold	100:107	arg1	scaffolds					15:23	Nature derived scaffolds	0:23	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.	0:213	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	21	theme	scaffold	100:107	arg1	Design					62:67	Design	62:67	Design	62:67	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	22	theme	green	203:207	arg1	leaf					209:212	green leaf	203:212	green leaf	203:212	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	5	23	dep	scaffolds	886:894	arg1	CgLA					880:883	CgLA	880:883	CgLA	880:883	These nanocrystals were incorporated to the formulations that were used to fabricate different chitosan-g-d,l-lactic acid (CgLA) scaffolds.
29054519	5	23	dep	scaffolds	886:894	arg1	acid					874:877	l-lactic acid	865:877	l-lactic acid (CgLA)	865:884	These nanocrystals were incorporated to the formulations that were used to fabricate different chitosan-g-d,l-lactic acid (CgLA) scaffolds.
29054519	2	24	theme	constant	399:406	arg1	stirring					408:415	constant stirring	399:415	constant stirring for 1h to obtain cellulose nanocrystals (CNCs)	399:462	The isolated cellulose was hydrolyzed using 64wt% H2SO4 at 55°C under constant stirring for 1h to obtain cellulose nanocrystals (CNCs).
29054519	0	25	theme	derived	7:13	arg1	scaffolds					15:23	Nature derived scaffolds	0:23	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.	0:213	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	25	theme	derived	7:13	arg1	fabrication					73:83	fabrication	73:83	fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf	73:212	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	25	theme	derived	7:13	arg1	Design					62:67	Design	62:67	Design	62:67	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	6	26	theme	composite	1214:1222	arg1	scaffolds					1224:1232	the fabricated composite scaffolds	1199:1232	the fabricated composite scaffolds	1199:1232	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	2	27	theme	isolated	333:340	arg1	cellulose					342:350	The isolated cellulose	329:350	The isolated cellulose	329:350	The isolated cellulose was hydrolyzed using 64wt% H2SO4 at 55°C under constant stirring for 1h to obtain cellulose nanocrystals (CNCs).
29054519	0	28	from	cv	200:201	arg1	chitosan-g-d					123:134	chitosan-g-d	123:134	chitosan-g-d	123:134	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	28	from	cv	200:201	arg1	acid					145:148	l-lactic acid	136:148	l-lactic acid	136:148	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	28	from	cv	200:201	arg1	nanocrystals					164:175	cellulose nanocrystals	154:175	cellulose nanocrystals from Lactuca sativa L. cv	154:201	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	6	29	theme	fabricated	1203:1212	arg1	scaffolds					1224:1232	the fabricated composite scaffolds	1199:1232	the fabricated composite scaffolds	1199:1232	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	8	30	theme	new	1454:1456	arg1	properties					1458:1467	new properties	1454:1467	new properties that are necessary for certain tissue engineering applications	1454:1530	Moreover, these characteristics can give new properties that are necessary for certain tissue engineering applications.
29054519	3	31	from	differences	551:561	arg1	characteristics					587:601	the physico-chemical characteristics	566:601	the physico-chemical characteristics of cellulose	566:614	Characterizations such as SEM, TEM, FTIR, TGA and XRD were done in order to determine differences in the physico-chemical characteristics of cellulose after each treatment step.
29054519	7	32	theme	scaffolds	1340:1348	arg1	properties					1319:1328	the properties	1315:1328	the properties of tissue scaffolds	1315:1348	These results can provide information on how nanofillers such as CNCs can alter the properties of tissue scaffolds through the chemical properties and interactions they provide.
29054519	7	33	theme	chemical	1362:1369	arg1	properties					1371:1380	the chemical properties	1358:1380	the chemical properties	1358:1380	These results can provide information on how nanofillers such as CNCs can alter the properties of tissue scaffolds through the chemical properties and interactions they provide.
29054519	6	34	from	increase	1102:1109	arg1	porosity					1114:1121	porosity	1114:1121	porosity	1114:1121	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	6	34	from	increase	1102:1109	arg1	concentration					1073:1085	the concentration	1069:1085	the concentration of CNCs added	1069:1099	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	2	35	theme	cellulose	434:442	arg1	CNCs					458:461	CNCs	458:461	CNCs	458:461	The isolated cellulose was hydrolyzed using 64wt% H2SO4 at 55°C under constant stirring for 1h to obtain cellulose nanocrystals (CNCs).
29054519	2	35	theme	cellulose	434:442	arg1	nanocrystals					444:455	cellulose nanocrystals	434:455	cellulose nanocrystals (CNCs)	434:462	The isolated cellulose was hydrolyzed using 64wt% H2SO4 at 55°C under constant stirring for 1h to obtain cellulose nanocrystals (CNCs).
29054519	6	36	theme	lactic	942:947	arg1	acid					949:952	lactic acid	942:952	lactic acid	942:952	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	5	37	used	used	824:827	arg2	formulations					801:812	the formulations	797:812	the formulations that were used to fabricate different chitosan-g-d,l-lactic acid (CgLA) scaffolds	797:894	These nanocrystals were incorporated to the formulations that were used to fabricate different chitosan-g-d,l-lactic acid (CgLA) scaffolds.
29054519	2	38	theme	64wt	373:376	arg1	H2SO4					379:383	64wt% H2SO4	373:383	64wt% H2SO4 at 55°C	373:391	The isolated cellulose was hydrolyzed using 64wt% H2SO4 at 55°C under constant stirring for 1h to obtain cellulose nanocrystals (CNCs).
29054519	0	39	theme	engineering	36:46	arg1	applications					48:59	tissue engineering applications	29:59	tissue engineering applications	29:59	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	40	theme	tissue	29:34	arg1	applications					48:59	tissue engineering applications	29:59	tissue engineering applications	29:59	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	41	theme	l-lactic	136:143	arg1	acid					145:148	l-lactic acid	136:148	l-lactic acid	136:148	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	3	42	theme	physico-chemical	570:585	arg1	characteristics					587:601	the physico-chemical characteristics	566:601	the physico-chemical characteristics of cellulose	566:614	Characterizations such as SEM, TEM, FTIR, TGA and XRD were done in order to determine differences in the physico-chemical characteristics of cellulose after each treatment step.
29054519	7	43	dep	interactions	1386:1397	arg1	provide					1404:1410	provide	1404:1410	provide	1404:1410	These results can provide information on how nanofillers such as CNCs can alter the properties of tissue scaffolds through the chemical properties and interactions they provide.
29054519	0	44	theme	cellulose	154:162	arg1	nanocrystals					164:175	cellulose nanocrystals	154:175	cellulose nanocrystals from Lactuca sativa L. cv	154:201	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	1	45	dep	extraction	234:243	arg1	cellulose					292:300	cellulose	292:300	cellulose	292:300	Through exhaustive extraction via successive alkali and bleaching treatments cellulose was isolated from lettuce.
29054519	7	46	theme	tissue	1333:1338	arg1	scaffolds					1340:1348	tissue scaffolds	1333:1348	tissue scaffolds	1333:1348	These results can provide information on how nanofillers such as CNCs can alter the properties of tissue scaffolds through the chemical properties and interactions they provide.
29054519	2	47	from	55°C	388:391	arg1	H2SO4					379:383	64wt% H2SO4	373:383	64wt% H2SO4 at 55°C	373:391	The isolated cellulose was hydrolyzed using 64wt% H2SO4 at 55°C under constant stirring for 1h to obtain cellulose nanocrystals (CNCs).
29054519	4	48	theme	33±12	693:697	arg1	dimensions					671:680	mean dimensions	666:680	mean dimensions of 237±26, 33±12 and 32±7nm	666:708	The isolated CNCs have mean dimensions of 237±26, 33±12 and 32±7nm in length, thickness and height, respectively.
29054519	6	49	theme	drug	1139:1142	arg1	property					1152:1159	drug release property	1139:1159	drug release property	1139:1159	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	3	50	theme	treatment	627:635	arg1	step					637:640	each treatment step	622:640	each treatment step	622:640	Characterizations such as SEM, TEM, FTIR, TGA and XRD were done in order to determine differences in the physico-chemical characteristics of cellulose after each treatment step.
29054519	5	51	dep	different	842:850	arg1	chitosan-g-d					852:863	chitosan-g-d	852:863	chitosan-g-d	852:863	These nanocrystals were incorporated to the formulations that were used to fabricate different chitosan-g-d,l-lactic acid (CgLA) scaffolds.
29054519	6	52	theme	linkage	903:909	arg1	formation					911:919	Amide linkage formation	897:919	Amide linkage formation between chitosan and lactic acid and further removal of water	897:981	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	4	53	contain	have	661:664	arg1	CNCs					656:659	The isolated CNCs	643:659	The isolated CNCs	643:659	The isolated CNCs have mean dimensions of 237±26, 33±12 and 32±7nm in length, thickness and height, respectively.
29054519	4	53	contain	have	661:664	arg2	dimensions					671:680	mean dimensions	666:680	mean dimensions of 237±26, 33±12 and 32±7nm	666:708	The isolated CNCs have mean dimensions of 237±26, 33±12 and 32±7nm in length, thickness and height, respectively.
29054519	3	54	theme	cellulose	606:614	arg1	characteristics					587:601	the physico-chemical characteristics	566:601	the physico-chemical characteristics of cellulose	566:614	Characterizations such as SEM, TEM, FTIR, TGA and XRD were done in order to determine differences in the physico-chemical characteristics of cellulose after each treatment step.
29054519	4	55	theme	32±7nm	703:708	arg1	dimensions					671:680	mean dimensions	666:680	mean dimensions of 237±26, 33±12 and 32±7nm	666:708	The isolated CNCs have mean dimensions of 237±26, 33±12 and 32±7nm in length, thickness and height, respectively.
29054519	1	56	theme	exhaustive	223:232	arg1	extraction					234:243	exhaustive extraction	223:243	exhaustive extraction via successive alkali	223:265	Through exhaustive extraction via successive alkali and bleaching treatments cellulose was isolated from lettuce.
29054519	0	57	dep	scaffolds	15:23	arg1	fabrication					73:83	fabrication	73:83	fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf	73:212	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	57	dep	scaffolds	15:23	arg1	scaffolds					15:23	Nature derived scaffolds	0:23	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.	0:213	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	0	57	dep	scaffolds	15:23	arg1	Design					62:67	Design	62:67	Design	62:67	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	6	58	theme	CNCs	1090:1093	arg1	concentration					1073:1085	the concentration	1069:1085	the concentration of CNCs added	1069:1099	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	0	59	dep	derived	7:13	arg1	Nature					0:5	Nature	0:5	Nature	0:5	Nature derived scaffolds for tissue engineering applications: Design and fabrication of a composite scaffold incorporating chitosan-g-d,l-lactic acid and cellulose nanocrystals from Lactuca sativa L. cv green leaf.
29054519	6	60	from	increase	1057:1064	arg1	porosity					1114:1121	porosity	1114:1121	porosity	1114:1121	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	6	60	from	increase	1057:1064	arg1	concentration					1073:1085	the concentration	1069:1085	the concentration of CNCs added	1069:1099	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	5	61	theme	l-lactic	865:872	arg1	CgLA					880:883	CgLA	880:883	CgLA	880:883	These nanocrystals were incorporated to the formulations that were used to fabricate different chitosan-g-d,l-lactic acid (CgLA) scaffolds.
29054519	5	61	theme	l-lactic	865:872	arg1	acid					874:877	l-lactic acid	865:877	l-lactic acid (CgLA)	865:884	These nanocrystals were incorporated to the formulations that were used to fabricate different chitosan-g-d,l-lactic acid (CgLA) scaffolds.
29054519	5	62	theme	different	842:850	arg1	scaffolds					886:894	different chitosan-g-d,l-lactic acid (CgLA) scaffolds	842:894	scaffolds	886:894	These nanocrystals were incorporated to the formulations that were used to fabricate different chitosan-g-d,l-lactic acid (CgLA) scaffolds.
29054519	6	63	theme	Amide	897:901	arg1	formation					911:919	Amide linkage formation	897:919	Amide linkage formation between chitosan and lactic acid and further removal of water	897:981	Amide linkage formation between chitosan and lactic acid and further removal of water was facilitated by oven-drying under vacuum at 80°C. Results show that an increase in the concentration of CNCs added, increase in porosity, degradability, drug release property and cell viability were observed from the fabricated composite scaffolds.
29054519	4	64	theme	mean	666:669	arg1	dimensions					671:680	mean dimensions	666:680	mean dimensions of 237±26, 33±12 and 32±7nm	666:708	The isolated CNCs have mean dimensions of 237±26, 33±12 and 32±7nm in length, thickness and height, respectively.
29054519	1	65	theme	successive	249:258	arg1	alkali					260:265	successive alkali	249:265	successive alkali	249:265	Through exhaustive extraction via successive alkali and bleaching treatments cellulose was isolated from lettuce.
26428116	5	0	theme	different	591:599	arg1	sites					610:614	two different sorption sites	587:614	two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material)	587:756	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	4	1	theme	Langmuir	533:540	arg1	equation					542:549	the Langmuir equation	529:549	the Langmuir equation	529:549	The sorption isotherm was described by the Langmuir equation.
26428116	5	2	theme	sorption	601:608	arg1	sites					610:614	two different sorption sites	587:614	two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material)	587:756	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	1	3	theme	ions	101:104	arg1	removal					84:90	The removal	80:90	The removal of As(V) ions from aqueous solution	80:126	The removal of As(V) ions from aqueous solution was carried out using composite sorbents based on chitosan (as the encapsulating material) and Cu(OH)2 or CuO.
26428116	8	4	theme	saline	979:984	arg1	solution					986:993	a saline solution	977:993	a saline solution (30 g L(-1) NaCl)	977:1011	Arsenic elution was performed using a saline solution (30 g L(-1) NaCl) at pH 12.
26428116	8	4	theme	saline	979:984	arg1	NaCl					1007:1010	30 g L(-1) NaCl	996:1010	30 g L(-1) NaCl	996:1010	Arsenic elution was performed using a saline solution (30 g L(-1) NaCl) at pH 12.
26428116	0	5	theme	composite	60:68	arg1	sorbents					70:77	chitosan/CuO composite sorbents	47:77	chitosan/CuO composite sorbents	47:77	Arsenic(V) sorption using chitosan/Cu(OH)2 and chitosan/CuO composite sorbents.
26428116	7	6	theme	temperature	851:861	arg1	effect					841:846	The effect	837:846	The effect of temperature	837:861	The effect of temperature was also evaluated, verifying the endothermic nature of the sorption process.
26428116	0	7	theme	chitosan/CuO	47:58	arg1	sorbents					70:77	chitosan/CuO composite sorbents	47:77	chitosan/CuO composite sorbents	47:77	Arsenic(V) sorption using chitosan/Cu(OH)2 and chitosan/CuO composite sorbents.
26428116	1	8	theme	encapsulating	195:207	arg1	material					209:216	the encapsulating material	191:216	the encapsulating material	191:216	The removal of As(V) ions from aqueous solution was carried out using composite sorbents based on chitosan (as the encapsulating material) and Cu(OH)2 or CuO.
26428116	7	9	theme	process	932:938	arg1	nature					909:914	the endothermic nature	893:914	the endothermic nature of the sorption process	893:938	The effect of temperature was also evaluated, verifying the endothermic nature of the sorption process.
26428116	8	10	theme	g	999:999	arg1	NaCl					1007:1010	30 g L(-1) NaCl	996:1010	30 g L(-1) NaCl	996:1010	Arsenic elution was performed using a saline solution (30 g L(-1) NaCl) at pH 12.
26428116	8	10	theme	g	999:999	arg1	solution					986:993	a saline solution	977:993	a saline solution (30 g L(-1) NaCl)	977:1011	Arsenic elution was performed using a saline solution (30 g L(-1) NaCl) at pH 12.
26428116	6	11	theme	order	816:820	arg1	equation					827:834	the pseudo-second order rate equation	798:834	the pseudo-second order rate equation	798:834	The uptake kinetics was well fitted by the pseudo-second order rate equation.
26428116	4	12	theme	sorption	494:501	arg1	isotherm					503:510	The sorption isotherm	490:510	The sorption isotherm	490:510	The sorption isotherm was described by the Langmuir equation.
26428116	3	13	theme	sorbent	404:410	arg1	dosage					412:417	sorbent dosage	404:417	sorbent dosage (SD)	404:422	Sorption uptake was highly dependent on pH, temperature, initial As(V) concentration and sorbent dosage (SD): the optimum initial pH for arsenic removal was found close to 4.
26428116	3	13	theme	sorbent	404:410	arg1	SD					420:421	SD	420:421	SD	420:421	Sorption uptake was highly dependent on pH, temperature, initial As(V) concentration and sorbent dosage (SD): the optimum initial pH for arsenic removal was found close to 4.
26428116	1	14	theme	aqueous	111:117	arg1	solution					119:126	aqueous solution	111:126	aqueous solution	111:126	The removal of As(V) ions from aqueous solution was carried out using composite sorbents based on chitosan (as the encapsulating material) and Cu(OH)2 or CuO.
26428116	5	15	dep	sites	610:614	arg1	one					617:619	one	617:619	one	617:619	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	5	15	dep	sites	610:614	arg1	other					690:694	other	690:694	other	690:694	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	3	16	theme	Sorption	315:322	arg1	uptake					324:329	Sorption uptake	315:329	Sorption uptake	315:329	Sorption uptake was highly dependent on pH, temperature, initial As(V) concentration and sorbent dosage (SD): the optimum initial pH for arsenic removal was found close to 4.
26428116	0	17	theme	Arsenic	0:6	arg1	V					8:8	V	8:8	V	8:8	Arsenic(V) sorption using chitosan/Cu(OH)2 and chitosan/CuO composite sorbents.
26428116	0	17	theme	Arsenic	0:6	arg1	sorption					11:18	Arsenic(V) sorption	0:18	Arsenic(V) sorption	0:18	Arsenic(V) sorption using chitosan/Cu(OH)2 and chitosan/CuO composite sorbents.
26428116	6	18	theme	pseudo-second	802:814	arg1	equation					827:834	the pseudo-second order rate equation	798:834	the pseudo-second order rate equation	798:834	The uptake kinetics was well fitted by the pseudo-second order rate equation.
26428116	5	19	theme	strong	630:635	arg1	affinity					637:644	a strong affinity	628:644	a strong affinity for As(V) (probably Cu(OH)2 or CuO)	628:680	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	8	20	theme	L	1001:1001	arg1	NaCl					1007:1010	30 g L(-1) NaCl	996:1010	30 g L(-1) NaCl	996:1010	Arsenic elution was performed using a saline solution (30 g L(-1) NaCl) at pH 12.
26428116	8	20	theme	L	1001:1001	arg1	solution					986:993	a saline solution	977:993	a saline solution (30 g L(-1) NaCl)	977:1011	Arsenic elution was performed using a saline solution (30 g L(-1) NaCl) at pH 12.
26428116	8	21	theme	Arsenic	941:947	arg1	elution					949:955	Arsenic elution	941:955	Arsenic elution	941:955	Arsenic elution was performed using a saline solution (30 g L(-1) NaCl) at pH 12.
26428116	5	22	theme	lower	705:709	arg1	affinity					711:718	a lower affinity	703:718	a lower affinity (probably the encapsulating material)	703:756	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	5	23	contain	having	696:701	arg1	other					690:694	other	690:694	other	690:694	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	5	23	contain	having	696:701	arg2	affinity					711:718	a lower affinity	703:718	a lower affinity (probably the encapsulating material)	703:756	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	5	24	dep	As	650:651	arg1	OH					669:670	OH	669:670	OH	669:670	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	5	24	dep	As	650:651	arg1	Cu					666:667	Cu	666:667	Cu(OH)2	666:672	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	5	24	dep	As	650:651	arg1	CuO					677:679	CuO	677:679	CuO	677:679	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	9	25	theme	sorbent	1044:1050	arg1	recycling					1027:1035	The recycling	1023:1035	The recycling of the sorbent	1023:1050	The recycling of the sorbent was tested, maintaining a removal efficiency and a metal recovery over 95% for five successive sorption/desorption cycles.
26428116	7	26	theme	sorption	923:930	arg1	process					932:938	the sorption process	919:938	the sorption process	919:938	The effect of temperature was also evaluated, verifying the endothermic nature of the sorption process.
26428116	2	27	theme	potential	295:303	arg1	analysis					305:312	Zeta potential analysis	290:312	Zeta potential analysis	290:312	The sorbents were characterized using SEM, EDX and Zeta potential analysis.
26428116	7	28	theme	endothermic	897:907	arg1	nature					909:914	the endothermic nature	893:914	the endothermic nature of the sorption process	893:938	The effect of temperature was also evaluated, verifying the endothermic nature of the sorption process.
26428116	5	29	theme	metal	556:560	arg1	ion					562:564	The metal ion	552:564	The metal ion	552:564	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	1	30	theme	composite	150:158	arg1	sorbents					160:167	composite sorbents	150:167	composite sorbents based on chitosan (as the encapsulating material) and Cu(OH)2 or CuO	150:236	The removal of As(V) ions from aqueous solution was carried out using composite sorbents based on chitosan (as the encapsulating material) and Cu(OH)2 or CuO.
26428116	3	31	theme	optimum	429:435	arg1	pH					445:446	the optimum initial pH	425:446	the optimum initial pH for arsenic removal	425:466	Sorption uptake was highly dependent on pH, temperature, initial As(V) concentration and sorbent dosage (SD): the optimum initial pH for arsenic removal was found close to 4.
26428116	3	32	theme	initial	372:378	arg1	V					383:383	V	383:383	V	383:383	Sorption uptake was highly dependent on pH, temperature, initial As(V) concentration and sorbent dosage (SD): the optimum initial pH for arsenic removal was found close to 4.
26428116	3	32	theme	initial	372:378	arg1	concentration					386:398	initial As(V) concentration	372:398	initial As(V) concentration	372:398	Sorption uptake was highly dependent on pH, temperature, initial As(V) concentration and sorbent dosage (SD): the optimum initial pH for arsenic removal was found close to 4.
26428116	5	33	contain	having	621:626	arg1	one					617:619	one	617:619	one	617:619	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	5	33	contain	having	621:626	arg2	affinity					637:644	a strong affinity	628:644	a strong affinity for As(V) (probably Cu(OH)2 or CuO)	628:680	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	3	34	theme	initial	437:443	arg1	pH					445:446	the optimum initial pH	425:446	the optimum initial pH for arsenic removal	425:466	Sorption uptake was highly dependent on pH, temperature, initial As(V) concentration and sorbent dosage (SD): the optimum initial pH for arsenic removal was found close to 4.
26428116	9	35	theme	sorption/desorption	1147:1165	arg1	cycles					1167:1172	five successive sorption/desorption cycles	1131:1172	five successive sorption/desorption cycles	1131:1172	The recycling of the sorbent was tested, maintaining a removal efficiency and a metal recovery over 95% for five successive sorption/desorption cycles.
26428116	3	36	theme	As	380:381	arg1	V					383:383	V	383:383	V	383:383	Sorption uptake was highly dependent on pH, temperature, initial As(V) concentration and sorbent dosage (SD): the optimum initial pH for arsenic removal was found close to 4.
26428116	3	36	theme	As	380:381	arg1	concentration					386:398	initial As(V) concentration	372:398	initial As(V) concentration	372:398	Sorption uptake was highly dependent on pH, temperature, initial As(V) concentration and sorbent dosage (SD): the optimum initial pH for arsenic removal was found close to 4.
26428116	5	37	theme	encapsulating	734:746	arg1	material					748:755	the encapsulating material	730:755	probably the encapsulating material	721:755	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	5	38	dep	affinity	711:718	arg1	material					748:755	the encapsulating material	730:755	probably the encapsulating material	721:755	The metal ion can be bound through two different sorption sites: one having a strong affinity for As(V) (probably Cu(OH)2 or CuO) and the other having a lower affinity (probably the encapsulating material).
26428116	9	39	theme	metal	1103:1107	arg1	recovery					1109:1116	a metal recovery	1101:1116	a metal recovery	1101:1116	The recycling of the sorbent was tested, maintaining a removal efficiency and a metal recovery over 95% for five successive sorption/desorption cycles.
26428116	1	40	from	solution	119:126	arg1	removal					84:90	The removal	80:90	The removal of As(V) ions from aqueous solution	80:126	The removal of As(V) ions from aqueous solution was carried out using composite sorbents based on chitosan (as the encapsulating material) and Cu(OH)2 or CuO.
26428116	1	41	theme	As	95:96	arg1	ions					101:104	As(V) ions	95:104	As(V) ions	95:104	The removal of As(V) ions from aqueous solution was carried out using composite sorbents based on chitosan (as the encapsulating material) and Cu(OH)2 or CuO.
26428116	9	42	theme	removal	1078:1084	arg1	efficiency					1086:1095	a removal efficiency	1076:1095	a removal efficiency	1076:1095	The recycling of the sorbent was tested, maintaining a removal efficiency and a metal recovery over 95% for five successive sorption/desorption cycles.
26428116	2	43	theme	Zeta	290:293	arg1	analysis					305:312	Zeta potential analysis	290:312	Zeta potential analysis	290:312	The sorbents were characterized using SEM, EDX and Zeta potential analysis.
26428116	3	44	theme	arsenic	452:458	arg1	removal					460:466	arsenic removal	452:466	arsenic removal	452:466	Sorption uptake was highly dependent on pH, temperature, initial As(V) concentration and sorbent dosage (SD): the optimum initial pH for arsenic removal was found close to 4.
26428116	9	45	theme	successive	1136:1145	arg1	cycles					1167:1172	five successive sorption/desorption cycles	1131:1172	five successive sorption/desorption cycles	1131:1172	The recycling of the sorbent was tested, maintaining a removal efficiency and a metal recovery over 95% for five successive sorption/desorption cycles.
26428116	6	46	theme	rate	822:825	arg1	equation					827:834	the pseudo-second order rate equation	798:834	the pseudo-second order rate equation	798:834	The uptake kinetics was well fitted by the pseudo-second order rate equation.
26428116	6	47	theme	uptake	763:768	arg1	kinetics					770:777	The uptake kinetics	759:777	The uptake kinetics	759:777	The uptake kinetics was well fitted by the pseudo-second order rate equation.
29136900	9	0	theme	sample	1336:1341	arg1	matrixes					1343:1350	various real sample matrixes	1323:1350	various real sample matrixes	1323:1350	S. cerevisiae content in various real sample matrixes are quantified by using this protocol.
29136900	8	1	theme	cell	1196:1199	arg1	content					1179:1185	the content	1175:1185	the content of yeast cell	1175:1199	The fluorescence intensity is linearly correlated with the content of yeast cell, showing a calibration graph of F = 3.01log[C]+11.7, offering a detection limit of 5×102 CFU/mL.
29136900	7	2	theme	yeast/	942:947	arg1	composite					986:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite	938:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite	938:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite is then isolated with an external magnet and the fluorescence from BbimDCN-OCDs incorporated in S. cerevisiae is monitored.
29136900	5	3	theme	cerevisiae	825:834	arg1	recognition/isolation					797:817	exclusive recognition/isolation	787:817	exclusive recognition/isolation of S. cerevisiae	787:834	The electrostatic attraction between positively charged magnetic nanoparticles and negatively charged yeast cells facilitates exclusive recognition/isolation of S. cerevisiae.
29136900	3	4	theme	yeast	484:488	arg1	cerevisiae					509:518	yeast cell Saccharomyces cerevisiae	484:518	yeast cell Saccharomyces cerevisiae	484:518	BbimDCN-OCDs exhibit favorable biocompability and excellent imaging capacity for fluorescence labelling of yeast cell Saccharomyces cerevisiae.
29136900	5	5	theme	yeast	763:767	arg1	cells					769:773	negatively charged yeast cells	744:773	negatively charged yeast cells	744:773	The electrostatic attraction between positively charged magnetic nanoparticles and negatively charged yeast cells facilitates exclusive recognition/isolation of S. cerevisiae.
29136900	2	6	theme	due	321:323	arg1	BbimDCN-OCDs					307:318	BbimDCN-OCDs	307:318	BbimDCN-OCDs	307:318	A yield of as high as 58% is obtained for the carbon dots, shortly termed as BbimDCN-OCDs, due to the presence of thermal-instable N(CN)2- moiety.
29136900	9	7	dep	S.	1298:1299	arg1	cerevisiae					1301:1310	cerevisiae	1301:1310	cerevisiae	1301:1310	S. cerevisiae content in various real sample matrixes are quantified by using this protocol.
29136900	1	8	dep	liquid	157:162	arg1	BbimDCN					203:209	BbimDCN	203:209	BbimDCN	203:209	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	1	8	dep	liquid	157:162	arg1	dicyandiamide					188:200	1,3-dibutylimidazolium dicyandiamide	165:200	1,3-dibutylimidazolium dicyandiamide	165:200	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	1	9	theme	1,3-dibutylimidazolium	165:186	arg1	BbimDCN					203:209	BbimDCN	203:209	BbimDCN	203:209	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	1	9	theme	1,3-dibutylimidazolium	165:186	arg1	dicyandiamide					188:200	1,3-dibutylimidazolium dicyandiamide	165:200	1,3-dibutylimidazolium dicyandiamide	165:200	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	8	10	theme	3.01log[C	1237:1245	arg1	graph					1224:1228	a calibration graph	1210:1228	a calibration graph of F = 3.01log[C]+11.7	1210:1251	The fluorescence intensity is linearly correlated with the content of yeast cell, showing a calibration graph of F = 3.01log[C]+11.7, offering a detection limit of 5×102 CFU/mL.
29136900	3	11	theme	imaging	437:443	arg1	capacity					445:452	excellent imaging capacity	427:452	excellent imaging capacity	427:452	BbimDCN-OCDs exhibit favorable biocompability and excellent imaging capacity for fluorescence labelling of yeast cell Saccharomyces cerevisiae.
29136900	3	12	theme	cerevisiae	509:518	arg1	labelling					471:479	fluorescence labelling	458:479	fluorescence labelling of yeast cell Saccharomyces cerevisiae	458:518	BbimDCN-OCDs exhibit favorable biocompability and excellent imaging capacity for fluorescence labelling of yeast cell Saccharomyces cerevisiae.
29136900	8	13	theme	CFU/mL	1290:1295	arg1	limit					1275:1279	a detection limit	1263:1279	a detection limit of 5×102 CFU/mL	1263:1295	The fluorescence intensity is linearly correlated with the content of yeast cell, showing a calibration graph of F = 3.01log[C]+11.7, offering a detection limit of 5×102 CFU/mL.
29136900	5	14	theme	exclusive	787:795	arg1	recognition/isolation					797:817	exclusive recognition/isolation	787:817	exclusive recognition/isolation of S. cerevisiae	787:834	The electrostatic attraction between positively charged magnetic nanoparticles and negatively charged yeast cells facilitates exclusive recognition/isolation of S. cerevisiae.
29136900	8	15	theme	fluorescence	1124:1135	arg1	intensity					1137:1145	The fluorescence intensity	1120:1145	The fluorescence intensity	1120:1145	The fluorescence intensity is linearly correlated with the content of yeast cell, showing a calibration graph of F = 3.01log[C]+11.7, offering a detection limit of 5×102 CFU/mL.
29136900	9	16	from	content	1312:1318	arg1	matrixes					1343:1350	various real sample matrixes	1323:1350	various real sample matrixes	1323:1350	S. cerevisiae content in various real sample matrixes are quantified by using this protocol.
29136900	2	17	theme	N	361:361	arg1	moiety					369:374	thermal-instable N(CN)2- moiety	344:374	thermal-instable N(CN)2- moiety	344:374	A yield of as high as 58% is obtained for the carbon dots, shortly termed as BbimDCN-OCDs, due to the presence of thermal-instable N(CN)2- moiety.
29136900	8	18	theme	calibration	1212:1222	arg1	graph					1224:1228	a calibration graph	1210:1228	a calibration graph of F = 3.01log[C]+11.7	1210:1251	The fluorescence intensity is linearly correlated with the content of yeast cell, showing a calibration graph of F = 3.01log[C]+11.7, offering a detection limit of 5×102 CFU/mL.
29136900	1	19	theme	Hydrophobic	84:94	arg1	source					222:227	carbon source	215:227	carbon source	215:227	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	1	19	theme	Hydrophobic	84:94	arg1	dots					118:121	Hydrophobic nitrogen-doped carbon dots	84:121	Hydrophobic nitrogen-doped carbon dots	84:121	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	2	20	theme	thermal-instable	344:359	arg1	moiety					369:374	thermal-instable N(CN)2- moiety	344:374	thermal-instable N(CN)2- moiety	344:374	A yield of as high as 58% is obtained for the carbon dots, shortly termed as BbimDCN-OCDs, due to the presence of thermal-instable N(CN)2- moiety.
29136900	4	21	theme	chitosan-modified	534:550	arg1	nanoparticles					572:584	chitosan-modified Dy3+-doped magnetic nanoparticles	534:584	chitosan-modified Dy3+-doped magnetic nanoparticles (shortly as Chitosan@Fe2.75Dy0.25O4) with superparamagnetism	534:645	In addition, chitosan-modified Dy3+-doped magnetic nanoparticles (shortly as Chitosan@Fe2.75Dy0.25O4) with superparamagnetism are prepared.
29136900	1	22	theme	nitrogen-doped	96:109	arg1	source					222:227	carbon source	215:227	carbon source	215:227	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	1	22	theme	nitrogen-doped	96:109	arg1	dots					118:121	Hydrophobic nitrogen-doped carbon dots	84:121	Hydrophobic nitrogen-doped carbon dots	84:121	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	7	23	from	BbimDCN-OCDs	1063:1074	arg1	fluorescence					1045:1056	the fluorescence	1041:1056	the fluorescence from BbimDCN-OCDs incorporated in S. cerevisiae	1041:1104	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite is then isolated with an external magnet and the fluorescence from BbimDCN-OCDs incorporated in S. cerevisiae is monitored.
29136900	0	24	theme	Saccharomyces	19:31	arg1	cerevisiae					33:42	yeast Saccharomyces cerevisiae	13:42	yeast Saccharomyces cerevisiae	13:42	Detection of yeast Saccharomyces cerevisiae with ionic liquid mediated carbon dots.
29136900	8	25	theme	=	1235:1235	arg1	3.01log[C					1237:1245	F = 3.01log[C]+11.7	1233:1251	F = 3.01log[C]+11.7	1233:1251	The fluorescence intensity is linearly correlated with the content of yeast cell, showing a calibration graph of F = 3.01log[C]+11.7, offering a detection limit of 5×102 CFU/mL.
29136900	7	26	theme	external	1021:1028	arg1	magnet					1030:1035	an external magnet	1018:1035	an external magnet	1018:1035	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite is then isolated with an external magnet and the fluorescence from BbimDCN-OCDs incorporated in S. cerevisiae is monitored.
29136900	1	27	theme	carbon	111:116	arg1	source					222:227	carbon source	215:227	carbon source	215:227	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	1	27	theme	carbon	111:116	arg1	dots					118:121	Hydrophobic nitrogen-doped carbon dots	84:121	Hydrophobic nitrogen-doped carbon dots	84:121	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	2	28	theme	moiety	369:374	arg1	presence					332:339	the presence	328:339	the presence of thermal-instable N(CN)2- moiety	328:374	A yield of as high as 58% is obtained for the carbon dots, shortly termed as BbimDCN-OCDs, due to the presence of thermal-instable N(CN)2- moiety.
29136900	0	29	theme	yeast	13:17	arg1	cerevisiae					33:42	yeast Saccharomyces cerevisiae	13:42	yeast Saccharomyces cerevisiae	13:42	Detection of yeast Saccharomyces cerevisiae with ionic liquid mediated carbon dots.
29136900	4	30	theme	magnetic	563:570	arg1	nanoparticles					572:584	chitosan-modified Dy3+-doped magnetic nanoparticles	534:584	chitosan-modified Dy3+-doped magnetic nanoparticles (shortly as Chitosan@Fe2.75Dy0.25O4) with superparamagnetism	534:645	In addition, chitosan-modified Dy3+-doped magnetic nanoparticles (shortly as Chitosan@Fe2.75Dy0.25O4) with superparamagnetism are prepared.
29136900	6	31	theme	@	921:921	arg1	Fe2.75Dy0.25O4					922:935	the Chitosan@Fe2.75Dy0.25O4	909:935	the Chitosan@Fe2.75Dy0.25O4	909:935	In practice, S. cerevisiae is labelled by BbimDCN-OCDs and adhered onto the Chitosan@Fe2.75Dy0.25O4.
29136900	2	32	theme	carbon	276:281	arg1	dots					283:286	the carbon dots	272:286	the carbon dots	272:286	A yield of as high as 58% is obtained for the carbon dots, shortly termed as BbimDCN-OCDs, due to the presence of thermal-instable N(CN)2- moiety.
29136900	1	33	theme	carbon	215:220	arg1	source					222:227	carbon source	215:227	carbon source	215:227	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	1	33	theme	carbon	215:220	arg1	dots					118:121	Hydrophobic nitrogen-doped carbon dots	84:121	Hydrophobic nitrogen-doped carbon dots	84:121	Hydrophobic nitrogen-doped carbon dots are prepared with energetic ionic liquid (1,3-dibutylimidazolium dicyandiamide, BbimDCN) as carbon source.
29136900	8	34	theme	detection	1265:1273	arg1	limit					1275:1279	a detection limit	1263:1279	a detection limit of 5×102 CFU/mL	1263:1295	The fluorescence intensity is linearly correlated with the content of yeast cell, showing a calibration graph of F = 3.01log[C]+11.7, offering a detection limit of 5×102 CFU/mL.
29136900	4	35	theme	Dy3+-doped	552:561	arg1	nanoparticles					572:584	chitosan-modified Dy3+-doped magnetic nanoparticles	534:584	chitosan-modified Dy3+-doped magnetic nanoparticles (shortly as Chitosan@Fe2.75Dy0.25O4) with superparamagnetism	534:645	In addition, chitosan-modified Dy3+-doped magnetic nanoparticles (shortly as Chitosan@Fe2.75Dy0.25O4) with superparamagnetism are prepared.
29136900	6	36	theme	Chitosan	913:920	arg1	Fe2.75Dy0.25O4					922:935	the Chitosan@Fe2.75Dy0.25O4	909:935	the Chitosan@Fe2.75Dy0.25O4	909:935	In practice, S. cerevisiae is labelled by BbimDCN-OCDs and adhered onto the Chitosan@Fe2.75Dy0.25O4.
29136900	7	37	theme	@	970:970	arg1	composite					986:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite	938:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite	938:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite is then isolated with an external magnet and the fluorescence from BbimDCN-OCDs incorporated in S. cerevisiae is monitored.
29136900	0	38	theme	cerevisiae	33:42	arg1	Detection					0:8	Detection	0:8	Detection of yeast Saccharomyces cerevisiae with ionic liquid	0:60	Detection of yeast Saccharomyces cerevisiae with ionic liquid mediated carbon dots.
29136900	5	39	theme	charged	755:761	arg1	cells					769:773	negatively charged yeast cells	744:773	negatively charged yeast cells	744:773	The electrostatic attraction between positively charged magnetic nanoparticles and negatively charged yeast cells facilitates exclusive recognition/isolation of S. cerevisiae.
29136900	2	40	theme	CN	363:364	arg1	moiety					369:374	thermal-instable N(CN)2- moiety	344:374	thermal-instable N(CN)2- moiety	344:374	A yield of as high as 58% is obtained for the carbon dots, shortly termed as BbimDCN-OCDs, due to the presence of thermal-instable N(CN)2- moiety.
29136900	8	41	theme	yeast	1190:1194	arg1	cell					1196:1199	yeast cell	1190:1199	yeast cell	1190:1199	The fluorescence intensity is linearly correlated with the content of yeast cell, showing a calibration graph of F = 3.01log[C]+11.7, offering a detection limit of 5×102 CFU/mL.
29136900	9	42	theme	S.	1298:1299	arg1	content					1312:1318	S. cerevisiae content	1298:1318	S. cerevisiae content in various real sample matrixes	1298:1350	S. cerevisiae content in various real sample matrixes are quantified by using this protocol.
29136900	3	43	theme	fluorescence	458:469	arg1	labelling					471:479	fluorescence labelling	458:479	fluorescence labelling of yeast cell Saccharomyces cerevisiae	458:518	BbimDCN-OCDs exhibit favorable biocompability and excellent imaging capacity for fluorescence labelling of yeast cell Saccharomyces cerevisiae.
29136900	5	44	theme	electrostatic	665:677	arg1	attraction					679:688	The electrostatic attraction	661:688	The electrostatic attraction between positively charged magnetic nanoparticles and negatively charged yeast cells	661:773	The electrostatic attraction between positively charged magnetic nanoparticles and negatively charged yeast cells facilitates exclusive recognition/isolation of S. cerevisiae.
29136900	7	45	theme	Fe2.75Dy0.25O4	971:984	arg1	composite					986:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite	938:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite	938:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite is then isolated with an external magnet and the fluorescence from BbimDCN-OCDs incorporated in S. cerevisiae is monitored.
29136900	5	46	theme	charged	709:715	arg1	nanoparticles					726:738	positively charged magnetic nanoparticles	698:738	positively charged magnetic nanoparticles	698:738	The electrostatic attraction between positively charged magnetic nanoparticles and negatively charged yeast cells facilitates exclusive recognition/isolation of S. cerevisiae.
29136900	4	47	dep	Fe2.75Dy0.25O4	607:620	arg1	shortly					587:593	shortly	587:593	shortly	587:593	In addition, chitosan-modified Dy3+-doped magnetic nanoparticles (shortly as Chitosan@Fe2.75Dy0.25O4) with superparamagnetism are prepared.
29136900	2	48	theme	%	254:254	arg1	yield					232:236	A yield	230:236	A yield of as high as 58%	230:254	A yield of as high as 58% is obtained for the carbon dots, shortly termed as BbimDCN-OCDs, due to the presence of thermal-instable N(CN)2- moiety.
29136900	0	49	with	Detection	0:8	arg1	liquid					55:60	liquid	55:60	liquid	55:60	Detection of yeast Saccharomyces cerevisiae with ionic liquid mediated carbon dots.
29136900	4	50	theme	@	606:606	arg1	Fe2.75Dy0.25O4					607:620	Chitosan@Fe2.75Dy0.25O4	598:620	Chitosan@Fe2.75Dy0.25O4	598:620	In addition, chitosan-modified Dy3+-doped magnetic nanoparticles (shortly as Chitosan@Fe2.75Dy0.25O4) with superparamagnetism are prepared.
29136900	5	51	theme	magnetic	717:724	arg1	nanoparticles					726:738	positively charged magnetic nanoparticles	698:738	positively charged magnetic nanoparticles	698:738	The electrostatic attraction between positively charged magnetic nanoparticles and negatively charged yeast cells facilitates exclusive recognition/isolation of S. cerevisiae.
29136900	7	52	theme	BbimDCN-OCDs/Chitosan	949:969	arg1	composite					986:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite	938:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite	938:994	The yeast/ BbimDCN-OCDs/Chitosan@Fe2.75Dy0.25O4 composite is then isolated with an external magnet and the fluorescence from BbimDCN-OCDs incorporated in S. cerevisiae is monitored.
29136900	4	53	theme	Chitosan	598:605	arg1	Fe2.75Dy0.25O4					607:620	Chitosan@Fe2.75Dy0.25O4	598:620	Chitosan@Fe2.75Dy0.25O4	598:620	In addition, chitosan-modified Dy3+-doped magnetic nanoparticles (shortly as Chitosan@Fe2.75Dy0.25O4) with superparamagnetism are prepared.
29136900	0	54	theme	carbon	71:76	arg1	dots					78:81	carbon dots	71:81	carbon dots	71:81	Detection of yeast Saccharomyces cerevisiae with ionic liquid mediated carbon dots.
29136900	9	55	theme	various	1323:1329	arg1	matrixes					1343:1350	various real sample matrixes	1323:1350	various real sample matrixes	1323:1350	S. cerevisiae content in various real sample matrixes are quantified by using this protocol.
29136900	3	56	theme	excellent	427:435	arg1	capacity					445:452	excellent imaging capacity	427:452	excellent imaging capacity	427:452	BbimDCN-OCDs exhibit favorable biocompability and excellent imaging capacity for fluorescence labelling of yeast cell Saccharomyces cerevisiae.
29136900	3	57	theme	cell	490:493	arg1	cerevisiae					509:518	yeast cell Saccharomyces cerevisiae	484:518	yeast cell Saccharomyces cerevisiae	484:518	BbimDCN-OCDs exhibit favorable biocompability and excellent imaging capacity for fluorescence labelling of yeast cell Saccharomyces cerevisiae.
29136900	3	58	theme	favorable	398:406	arg1	biocompability					408:421	favorable biocompability	398:421	favorable biocompability	398:421	BbimDCN-OCDs exhibit favorable biocompability and excellent imaging capacity for fluorescence labelling of yeast cell Saccharomyces cerevisiae.
29136900	9	59	theme	real	1331:1334	arg1	matrixes					1343:1350	various real sample matrixes	1323:1350	various real sample matrixes	1323:1350	S. cerevisiae content in various real sample matrixes are quantified by using this protocol.
29136900	4	60	with	nanoparticles	572:584	arg1	superparamagnetism					628:645	superparamagnetism	628:645	superparamagnetism	628:645	In addition, chitosan-modified Dy3+-doped magnetic nanoparticles (shortly as Chitosan@Fe2.75Dy0.25O4) with superparamagnetism are prepared.
29136900	3	61	theme	Saccharomyces	495:507	arg1	cerevisiae					509:518	yeast cell Saccharomyces cerevisiae	484:518	yeast cell Saccharomyces cerevisiae	484:518	BbimDCN-OCDs exhibit favorable biocompability and excellent imaging capacity for fluorescence labelling of yeast cell Saccharomyces cerevisiae.
28017763	2	0	from	time	408:411	arg1	X2					455:456	X2	455:456	X2	455:456	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	0	from	time	408:411	arg1	gelation					445:452	first gelation	439:452	first gelation (X2)	439:457	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	5	1	from	time	1201:1204	arg1	gelation					1245:1252	secondary gelation	1235:1252	secondary gelation	1235:1252	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	1	from	time	1201:1204	arg1	gelation					1172:1179	the first gelation	1162:1179	the first gelation	1162:1179	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	2	2	from	concentration	497:509	arg1	X2					455:456	X2	455:456	X2	455:456	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	2	from	concentration	497:509	arg1	gelation					445:452	first gelation	439:452	first gelation (X2)	439:457	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	3	3	theme	physical	983:990	arg1	properties					992:1001	physical properties	983:1001	physical properties	983:1001	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	2	4	from	capacity	746:753	arg1	Y4					726:727	Y4	726:727	Y4	726:727	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	4	from	capacity	746:753	arg1	fluid					730:734	small intestinal (Y4) fluid	708:734	small intestinal (Y4) fluid	708:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	4	from	capacity	746:753	arg1	gastric					691:697	gastric	691:697	gastric	691:697	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	4	from	capacity	746:753	arg1	Y3					700:701	Y3	700:701	Y3	700:701	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	5	5	theme	6.04min	1209:1215	arg1	CL					1229:1230	0.058M CL	1222:1230	0.058M CL in secondary gelation	1222:1252	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	5	theme	6.04min	1209:1215	arg1	conditions					1082:1091	The optimal conditions	1070:1091	The optimal conditions of LHB formulation	1070:1110	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	5	theme	6.04min	1209:1215	arg1	time					1201:1204	secondary gelation time	1182:1204	secondary gelation time of 6.04min	1182:1215	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	5	theme	6.04min	1209:1215	arg1	CL					1156:1157	0.13M CL	1150:1157	0.13M CL in the first gelation	1150:1179	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	5	theme	6.04min	1209:1215	arg1	time					1132:1135	first gelation time	1117:1135	first gelation time of 23.99min	1117:1147	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	6	6	theme	hardness	1310:1317	arg1	release					1350:1356	TP release	1347:1356	TP release	1347:1356	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	6	theme	hardness	1310:1317	arg1	25.5N					1301:1305	25.5N	1301:1305	25.5N of hardness	1301:1317	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	6	theme	hardness	1310:1317	arg1	LE					1330:1331	LE	1330:1331	LE	1330:1331	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	6	theme	hardness	1310:1317	arg1	hardness					1310:1317	hardness	1310:1317	hardness	1310:1317	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	6	theme	hardness	1310:1317	arg1	%					1342:1342	27.38%	1337:1342	27.38% of TP release	1337:1356	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	6	theme	hardness	1310:1317	arg1	%					1325:1325	85.67%	1320:1325	85.67% of LE	1320:1331	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	3	7	theme	variables	970:978	arg1	importance					935:944	the importance	931:944	the importance of the four independent variables to physical properties	931:1001	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	2	8	theme	physical	548:555	arg1	properties					557:566	seven physical properties	542:566	seven physical properties of LHB	542:573	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	5	9	theme	secondary	1182:1190	arg1	time					1201:1204	secondary gelation time	1182:1204	secondary gelation time of 6.04min	1182:1215	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	6	10	theme	LHB	1284:1286	arg1	formulation					1269:1279	The optimized formulation	1255:1279	The optimized formulation of LHB	1255:1286	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	3	11	theme	composite	821:829	arg1	design					831:836	a central composite design	811:836	a central composite design with response surface methodology	811:870	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	2	12	theme	total	648:652	arg1	TP					674:675	TP	674:675	TP	674:675	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	12	theme	total	648:652	arg1	compounds					663:671	total phenolic compounds	648:671	total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	648:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	0	13	theme	surface	143:149	arg1	methodology					151:161	response surface methodology	134:161	response surface methodology	134:161	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification: Optimization by response surface methodology.
28017763	2	14	theme	secondary	514:522	arg1	X4					534:535	X4	534:535	X4	534:535	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	14	theme	secondary	514:522	arg1	gelation					524:531	secondary gelation	514:531	secondary gelation (X4)	514:536	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	15	from	gelation	524:531	arg1	effect					355:360	The effect	351:360	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6),	351:780	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	6	16	dep	2.21	1423:1426	arg1	to					1420:1421	to	1420:1421	to	1420:1421	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	2	17	from	time	479:482	arg1	X2					455:456	X2	455:456	X2	455:456	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	17	from	time	479:482	arg1	gelation					445:452	first gelation	439:452	first gelation (X2)	439:457	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	5	18	theme	optimal	1074:1080	arg1	conditions					1082:1091	The optimal conditions	1070:1091	The optimal conditions of LHB formulation	1070:1110	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	18	theme	optimal	1074:1080	arg1	time					1132:1135	first gelation time	1117:1135	first gelation time of 23.99min	1117:1147	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	6	19	with	fluid	1379:1383	arg1	error-values					1400:1411	the small error-values	1390:1411	the small error-values (-2.47 to 2.21%)	1390:1428	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	19	with	fluid	1379:1383	arg1	%					1427:1427	-2.47 to 2.21%	1414:1427	-2.47 to 2.21%	1414:1427	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	1	20	theme	Liquid-core	164:174	arg1	LHB					192:194	LHB	192:194	LHB	192:194	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	1	20	theme	Liquid-core	164:174	arg1	beads					185:189	Liquid-core hydrogel beads	164:189	Liquid-core hydrogel beads (LHB)	164:195	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	5	21	theme	secondary	1235:1243	arg1	gelation					1245:1252	secondary gelation	1235:1252	secondary gelation	1235:1252	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	0	22	theme	by-products	79:89	arg1	compounds					59:67	functional compounds	48:67	functional compounds of radish by-products	48:89	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification: Optimization by response surface methodology.
28017763	1	23	theme	calcium	333:339	arg1	source					325:330	the common calcium source	306:330	the common calcium source	306:330	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	1	23	theme	calcium	333:339	arg1	chloride					341:348	calcium chloride	333:348	calcium chloride	333:348	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	2	24	theme	LHB	571:573	arg1	properties					557:566	seven physical properties	542:566	seven physical properties of LHB	542:573	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	0	25	theme	reverse	94:100	arg1	spherification					102:115	reverse spherification	94:115	reverse spherification	94:115	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification: Optimization by response surface methodology.
28017763	3	26	theme	surface	852:858	arg1	methodology					860:870	response surface methodology	843:870	response surface methodology	843:870	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	6	27	theme	gastric	1371:1377	arg1	fluid					1379:1383	simulated gastric fluid	1361:1383	simulated gastric fluid with the small error-values (-2.47 to 2.21%)	1361:1428	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	2	28	theme	gelation	470:477	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	28	theme	gelation	470:477	arg1	X3					485:486	X3	485:486	X3	485:486	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	28	theme	gelation	470:477	arg1	time					479:482	secondary gelation time	460:482	secondary gelation time (X3)	460:487	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	29	from	concentration	422:434	arg1	X2					455:456	X2	455:456	X2	455:456	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	29	from	concentration	422:434	arg1	gelation					445:452	first gelation	439:452	first gelation (X2)	439:457	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	4	30	theme	LHB	1033:1035	arg1	diameter					1021:1028	The diameter	1017:1028	The diameter of LHB	1017:1035	The diameter of LHB was in the range of 4.17-5.84mm.
28017763	5	31	theme	first	1117:1121	arg1	conditions					1082:1091	The optimal conditions	1070:1091	The optimal conditions of LHB formulation	1070:1110	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	31	theme	first	1117:1121	arg1	time					1132:1135	first gelation time	1117:1135	first gelation time of 23.99min	1117:1147	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	2	32	from	sphericity	765:774	arg1	Y4					726:727	Y4	726:727	Y4	726:727	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	32	from	sphericity	765:774	arg1	fluid					730:734	small intestinal (Y4) fluid	708:734	small intestinal (Y4) fluid	708:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	32	from	sphericity	765:774	arg1	gastric					691:697	gastric	691:697	gastric	691:697	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	32	from	sphericity	765:774	arg1	Y3					700:701	Y3	700:701	Y3	700:701	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	time					479:482	secondary gelation time	460:482	secondary gelation time (X3)	460:487	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	amount					638:643	release amount	630:643	release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	630:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	concentration					497:509	CL concentration	494:509	CL concentration	494:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	Y1					596:597	Y1	596:597	Y1	596:597	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	Y2					625:626	Y2	625:626	Y2	625:626	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	capacity					746:753	swelling capacity	737:753	swelling capacity (Y5)	737:758	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	time					408:411	first gelation time	393:411	first gelation time (X1)	393:416	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	sphericity					765:774	sphericity	765:774	sphericity	765:774	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	concentration					422:434	CL concentration	419:434	CL concentration in first gelation (X2)	419:457	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	compounds					663:671	total phenolic compounds	648:671	total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	648:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	Y6					777:778	Y6	777:778	Y6	777:778	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	hardness					586:593	hardness	586:593	hardness	586:593	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	33	theme	independent	370:380	arg1	efficiency					609:618	loading efficiency	601:618	loading efficiency	601:618	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	0	34	theme	Liquid-core	0:10	arg1	beads					30:34	Liquid-core alginate hydrogel beads	0:34	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification	0:115	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification: Optimization by response surface methodology.
28017763	6	35	theme	TP	1347:1348	arg1	release					1350:1356	TP release	1347:1356	TP release	1347:1356	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	2	36	theme	swelling	737:744	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	36	theme	swelling	737:744	arg1	Y5					756:757	Y5	756:757	Y5	756:757	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	36	theme	swelling	737:744	arg1	capacity					746:753	swelling capacity	737:753	swelling capacity (Y5)	737:758	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	0	37	theme	hydrogel	21:28	arg1	beads					30:34	Liquid-core alginate hydrogel beads	0:34	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification	0:115	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification: Optimization by response surface methodology.
28017763	3	38	with	design	831:836	arg1	methodology					860:870	response surface methodology	843:870	response surface methodology	843:870	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	3	39	theme	Y1-Y6	920:924	arg1	properties					909:918	LHB properties	905:918	LHB properties Y1-Y6	905:924	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	4	40	theme	4.17-5.84mm	1057:1067	arg1	range					1048:1052	the range	1044:1052	the range of 4.17-5.84mm	1044:1067	The diameter of LHB was in the range of 4.17-5.84mm.
28017763	5	41	theme	formulation	1100:1110	arg1	conditions					1082:1091	The optimal conditions	1070:1091	The optimal conditions of LHB formulation	1070:1110	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	41	theme	formulation	1100:1110	arg1	time					1132:1135	first gelation time	1117:1135	first gelation time of 23.99min	1117:1147	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	2	42	theme	gelation	399:406	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	42	theme	gelation	399:406	arg1	X1					414:415	X1	414:415	X1	414:415	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	42	theme	gelation	399:406	arg1	time					408:411	first gelation time	393:411	first gelation time (X1)	393:416	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	3	43	theme	LHB	905:907	arg1	properties					909:918	LHB properties	905:918	LHB properties Y1-Y6	905:924	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	0	44	theme	functional	48:57	arg1	compounds					59:67	functional compounds	48:67	functional compounds of radish by-products	48:89	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification: Optimization by response surface methodology.
28017763	1	45	theme	common	310:315	arg1	source					325:330	the common calcium source	306:330	the common calcium source	306:330	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	1	45	theme	common	310:315	arg1	chloride					341:348	calcium chloride	333:348	calcium chloride	333:348	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	5	46	theme	first	1166:1170	arg1	gelation					1172:1179	the first gelation	1162:1179	the first gelation	1162:1179	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	47	theme	23.99min	1140:1147	arg1	CL					1229:1230	0.058M CL	1222:1230	0.058M CL in secondary gelation	1222:1252	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	47	theme	23.99min	1140:1147	arg1	conditions					1082:1091	The optimal conditions	1070:1091	The optimal conditions of LHB formulation	1070:1110	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	47	theme	23.99min	1140:1147	arg1	time					1201:1204	secondary gelation time	1182:1204	secondary gelation time of 6.04min	1182:1215	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	47	theme	23.99min	1140:1147	arg1	CL					1156:1157	0.13M CL	1150:1157	0.13M CL in the first gelation	1150:1179	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	47	theme	23.99min	1140:1147	arg1	time					1132:1135	first gelation time	1117:1135	first gelation time of 23.99min	1117:1147	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	1	48	theme	sodium	248:253	arg1	alginate					255:262	sodium alginate	248:262	sodium alginate	248:262	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	3	49	theme	independent	958:968	arg1	variables					970:978	the four independent variables	949:978	the four independent variables	949:978	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	5	50	theme	0.13M	1150:1154	arg1	CL					1156:1157	0.13M CL	1150:1157	0.13M CL in the first gelation	1150:1179	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	2	51	from	fluid	730:734	arg1	TP					674:675	TP	674:675	TP	674:675	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	51	from	fluid	730:734	arg1	Y5					756:757	Y5	756:757	Y5	756:757	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	51	from	fluid	730:734	arg1	amount					638:643	release amount	630:643	release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	630:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	51	from	fluid	730:734	arg1	capacity					746:753	swelling capacity	737:753	swelling capacity (Y5)	737:758	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	51	from	fluid	730:734	arg1	sphericity					765:774	sphericity	765:774	sphericity	765:774	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	51	from	fluid	730:734	arg1	compounds					663:671	total phenolic compounds	648:671	total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	648:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	51	from	fluid	730:734	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	1	52	theme	calcium	274:280	arg1	CL					291:292	CL	291:292	CL	291:292	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	1	52	theme	calcium	274:280	arg1	lactate					282:288	calcium lactate	274:288	calcium lactate (CL)	274:293	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	2	53	theme	release	630:636	arg1	TP					674:675	TP	674:675	TP	674:675	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	53	theme	release	630:636	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	53	theme	release	630:636	arg1	compounds					663:671	total phenolic compounds	648:671	total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	648:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	53	theme	release	630:636	arg1	amount					638:643	release amount	630:643	release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	630:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	54	theme	intestinal	714:723	arg1	fluid					730:734	small intestinal (Y4) fluid	708:734	small intestinal (Y4) fluid	708:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	54	theme	intestinal	714:723	arg1	Y4					726:727	Y4	726:727	Y4	726:727	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	5	55	from	CL	1229:1230	arg1	gelation					1245:1252	secondary gelation	1235:1252	secondary gelation	1235:1252	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	55	from	CL	1229:1230	arg1	gelation					1172:1179	the first gelation	1162:1179	the first gelation	1162:1179	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	3	56	theme	central	813:819	arg1	design					831:836	a central composite design	811:836	a central composite design with response surface methodology	811:870	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	2	57	theme	phenolic	654:661	arg1	TP					674:675	TP	674:675	TP	674:675	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	57	theme	phenolic	654:661	arg1	compounds					663:671	total phenolic compounds	648:671	total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	648:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	0	58	theme	response	134:141	arg1	methodology					151:161	response surface methodology	134:161	response surface methodology	134:161	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification: Optimization by response surface methodology.
28017763	5	59	theme	gelation	1192:1199	arg1	time					1201:1204	secondary gelation time	1182:1204	secondary gelation time of 6.04min	1182:1215	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	6	60	theme	optimized	1259:1267	arg1	formulation					1269:1279	The optimized formulation	1255:1279	The optimized formulation of LHB	1255:1286	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	1	61	theme	reverse	221:227	arg1	spherification					229:242	reverse spherification	221:242	reverse spherification	221:242	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	2	62	theme	loading	601:607	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	62	theme	loading	601:607	arg1	efficiency					609:618	loading efficiency	601:618	loading efficiency	601:618	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	63	from	properties	557:566	arg1	effect					355:360	The effect	351:360	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6),	351:780	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	64	from	effect	355:360	arg1	X4					534:535	X4	534:535	X4	534:535	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	64	from	effect	355:360	arg1	gelation					524:531	secondary gelation	514:531	secondary gelation (X4)	514:536	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	64	from	effect	355:360	arg1	properties					557:566	seven physical properties	542:566	seven physical properties of LHB	542:573	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	6	65	theme	LE	1330:1331	arg1	release					1350:1356	TP release	1347:1356	TP release	1347:1356	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	65	theme	LE	1330:1331	arg1	25.5N					1301:1305	25.5N	1301:1305	25.5N of hardness	1301:1317	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	65	theme	LE	1330:1331	arg1	LE					1330:1331	LE	1330:1331	LE	1330:1331	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	65	theme	LE	1330:1331	arg1	hardness					1310:1317	hardness	1310:1317	hardness	1310:1317	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	65	theme	LE	1330:1331	arg1	%					1342:1342	27.38%	1337:1342	27.38% of TP release	1337:1356	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	65	theme	LE	1330:1331	arg1	%					1325:1325	85.67%	1320:1325	85.67% of LE	1320:1331	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	2	66	theme	CL	494:495	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	66	theme	CL	494:495	arg1	concentration					497:509	CL concentration	494:509	CL concentration	494:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	6	67	theme	small	1394:1398	arg1	error-values					1400:1411	the small error-values	1390:1411	the small error-values (-2.47 to 2.21%)	1390:1428	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	67	theme	small	1394:1398	arg1	%					1427:1427	-2.47 to 2.21%	1414:1427	-2.47 to 2.21%	1414:1427	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	5	68	from	time	1132:1135	arg1	gelation					1245:1252	secondary gelation	1235:1252	secondary gelation	1235:1252	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	68	from	time	1132:1135	arg1	gelation					1172:1179	the first gelation	1162:1179	the first gelation	1162:1179	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	0	69	theme	radish	72:77	arg1	by-products					79:89	radish by-products	72:89	radish by-products	72:89	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification: Optimization by response surface methodology.
28017763	1	70	theme	hydrogel	176:183	arg1	LHB					192:194	LHB	192:194	LHB	192:194	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	1	70	theme	hydrogel	176:183	arg1	beads					185:189	Liquid-core hydrogel beads	164:189	Liquid-core hydrogel beads (LHB)	164:195	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	3	71	theme	response	843:850	arg1	methodology					860:870	response surface methodology	843:870	response surface methodology	843:870	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	0	72	dep	Optimization	118:129	arg1	beads					30:34	Liquid-core alginate hydrogel beads	0:34	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification	0:115	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification: Optimization by response surface methodology.
28017763	3	73	used	used	876:879	arg2	design					831:836	a central composite design	811:836	a central composite design with response surface methodology	811:870	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	2	74	from	compounds	663:671	arg1	Y4					726:727	Y4	726:727	Y4	726:727	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	74	from	compounds	663:671	arg1	fluid					730:734	small intestinal (Y4) fluid	708:734	small intestinal (Y4) fluid	708:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	74	from	compounds	663:671	arg1	gastric					691:697	gastric	691:697	gastric	691:697	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	74	from	compounds	663:671	arg1	Y3					700:701	Y3	700:701	Y3	700:701	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	6	75	theme	simulated	1361:1369	arg1	fluid					1379:1383	simulated gastric fluid	1361:1383	simulated gastric fluid with the small error-values (-2.47 to 2.21%)	1361:1428	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	2	76	theme	secondary	460:468	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	76	theme	secondary	460:468	arg1	X3					485:486	X3	485:486	X3	485:486	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	76	theme	secondary	460:468	arg1	time					479:482	secondary gelation time	460:482	secondary gelation time (X3)	460:487	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	5	77	theme	0.058M	1222:1227	arg1	CL					1229:1230	0.058M CL	1222:1230	0.058M CL in secondary gelation	1222:1252	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	78	theme	gelation	1123:1130	arg1	conditions					1082:1091	The optimal conditions	1070:1091	The optimal conditions of LHB formulation	1070:1110	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	78	theme	gelation	1123:1130	arg1	time					1132:1135	first gelation time	1117:1135	first gelation time of 23.99min	1117:1147	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	2	79	theme	variables	382:390	arg1	effect					355:360	The effect	351:360	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6),	351:780	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	0	80	theme	alginate	12:19	arg1	beads					30:34	Liquid-core alginate hydrogel beads	0:34	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification	0:115	Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification: Optimization by response surface methodology.
28017763	6	81	theme	release	1350:1356	arg1	release					1350:1356	TP release	1347:1356	TP release	1347:1356	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	81	theme	release	1350:1356	arg1	25.5N					1301:1305	25.5N	1301:1305	25.5N of hardness	1301:1317	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	81	theme	release	1350:1356	arg1	LE					1330:1331	LE	1330:1331	LE	1330:1331	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	81	theme	release	1350:1356	arg1	hardness					1310:1317	hardness	1310:1317	hardness	1310:1317	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	81	theme	release	1350:1356	arg1	%					1342:1342	27.38%	1337:1342	27.38% of TP release	1337:1356	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	6	81	theme	release	1350:1356	arg1	%					1325:1325	85.67%	1320:1325	85.67% of LE	1320:1331	The optimized formulation of LHB demonstrated 25.5N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (-2.47 to 2.21%).
28017763	3	82	theme	properties	909:918	arg1	optimization					889:900	the optimization	885:900	the optimization of LHB properties Y1-Y6	885:924	Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1-Y6, and the importance of the four independent variables to physical properties was analyzed.
28017763	2	83	from	gastric	691:697	arg1	TP					674:675	TP	674:675	TP	674:675	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	83	from	gastric	691:697	arg1	Y5					756:757	Y5	756:757	Y5	756:757	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	83	from	gastric	691:697	arg1	amount					638:643	release amount	630:643	release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	630:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	83	from	gastric	691:697	arg1	capacity					746:753	swelling capacity	737:753	swelling capacity (Y5)	737:758	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	83	from	gastric	691:697	arg1	sphericity					765:774	sphericity	765:774	sphericity	765:774	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	83	from	gastric	691:697	arg1	compounds					663:671	total phenolic compounds	648:671	total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	648:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	83	from	gastric	691:697	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	5	84	theme	LHB	1096:1098	arg1	formulation					1100:1110	LHB formulation	1096:1110	LHB formulation	1096:1110	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	85	from	CL	1156:1157	arg1	gelation					1245:1252	secondary gelation	1235:1252	secondary gelation	1235:1252	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	5	85	from	CL	1156:1157	arg1	gelation					1172:1179	the first gelation	1162:1179	the first gelation	1162:1179	The optimal conditions of LHB formulation were first gelation time of 23.99min, 0.13M CL in the first gelation, secondary gelation time of 6.04min, and 0.058M CL in secondary gelation.
28017763	2	86	theme	first	439:443	arg1	X2					455:456	X2	455:456	X2	455:456	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	86	theme	first	439:443	arg1	gelation					445:452	first gelation	439:452	first gelation (X2)	439:457	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	87	theme	first	393:397	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	87	theme	first	393:397	arg1	X1					414:415	X1	414:415	X1	414:415	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	87	theme	first	393:397	arg1	time					408:411	first gelation time	393:411	first gelation time (X1)	393:416	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	1	88	theme	calcium	317:323	arg1	source					325:330	the common calcium source	306:330	the common calcium source	306:330	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	1	88	theme	calcium	317:323	arg1	chloride					341:348	calcium chloride	333:348	calcium chloride	333:348	Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride.
28017763	2	89	from	variables	382:390	arg1	X4					534:535	X4	534:535	X4	534:535	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	89	from	variables	382:390	arg1	gelation					524:531	secondary gelation	514:531	secondary gelation (X4)	514:536	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	89	from	variables	382:390	arg1	properties					557:566	seven physical properties	542:566	seven physical properties of LHB	542:573	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	90	theme	small	708:712	arg1	fluid					730:734	small intestinal (Y4) fluid	708:734	small intestinal (Y4) fluid	708:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	90	theme	small	708:712	arg1	Y4					726:727	Y4	726:727	Y4	726:727	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	91	from	amount	638:643	arg1	Y4					726:727	Y4	726:727	Y4	726:727	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	91	from	amount	638:643	arg1	fluid					730:734	small intestinal (Y4) fluid	708:734	small intestinal (Y4) fluid	708:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	91	from	amount	638:643	arg1	gastric					691:697	gastric	691:697	gastric	691:697	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	91	from	amount	638:643	arg1	Y3					700:701	Y3	700:701	Y3	700:701	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	92	theme	compounds	663:671	arg1	TP					674:675	TP	674:675	TP	674:675	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	92	theme	compounds	663:671	arg1	Y5					756:757	Y5	756:757	Y5	756:757	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	92	theme	compounds	663:671	arg1	amount					638:643	release amount	630:643	release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	630:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	92	theme	compounds	663:671	arg1	capacity					746:753	swelling capacity	737:753	swelling capacity (Y5)	737:758	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	92	theme	compounds	663:671	arg1	sphericity					765:774	sphericity	765:774	sphericity	765:774	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	92	theme	compounds	663:671	arg1	compounds					663:671	total phenolic compounds	648:671	total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid	648:734	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	92	theme	compounds	663:671	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	93	dep	variables	382:390	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	93	dep	variables	382:390	arg1	X1					414:415	X1	414:415	X1	414:415	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	93	dep	variables	382:390	arg1	time					479:482	secondary gelation time	460:482	secondary gelation time (X3)	460:487	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	93	dep	variables	382:390	arg1	concentration					422:434	CL concentration	419:434	CL concentration in first gelation (X2)	419:457	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	93	dep	variables	382:390	arg1	concentration					497:509	CL concentration	494:509	CL concentration	494:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	93	dep	variables	382:390	arg1	X3					485:486	X3	485:486	X3	485:486	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	93	dep	variables	382:390	arg1	time					408:411	first gelation time	393:411	first gelation time (X1)	393:416	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	93	dep	variables	382:390	arg1	diameter					576:583	diameter	576:583	diameter	576:583	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	94	theme	CL	419:420	arg1	variables					382:390	four independent variables	365:390	four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration	365:509	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
28017763	2	94	theme	CL	419:420	arg1	concentration					422:434	CL concentration	419:434	CL concentration in first gelation (X2)	419:457	The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated.
26432372	0	0	theme	adsorbent	75:83	arg1	solid-phase					63:73	a novel solid-phase	55:73	a novel solid-phase adsorbent for removal of congo red	55:108	Schiff base - Chitosan grafted L-monoguluronic acid as a novel solid-phase adsorbent for removal of congo red.
26432372	0	0	theme	adsorbent	75:83	arg1	acid					47:50	L-monoguluronic acid	31:50	L-monoguluronic acid	31:50	Schiff base - Chitosan grafted L-monoguluronic acid as a novel solid-phase adsorbent for removal of congo red.
26432372	7	1	theme	treatment	1156:1164	arg1	process					1166:1172	a wastewater treatment process	1143:1172	a wastewater treatment process	1143:1172	SBCS would be a good method and resource to increase absorption efficiency for the removal of anionic dyes in a wastewater treatment process.
26432372	1	2	theme	adsorbent	137:145	arg1	chitosan					128:135	A novel modified chitosan adsorbent	111:145	A novel modified chitosan adsorbent (GL-SBCS)	111:155	A novel modified chitosan adsorbent (GL-SBCS) was synthesized by covalently grafting a Schiff base-chitosan (SBCS) onto the surface of l-monoguluronic acid.
26432372	1	2	theme	adsorbent	137:145	arg1	GL-SBCS					148:154	GL-SBCS	148:154	GL-SBCS	148:154	A novel modified chitosan adsorbent (GL-SBCS) was synthesized by covalently grafting a Schiff base-chitosan (SBCS) onto the surface of l-monoguluronic acid.
26432372	6	3	theme	sodium	941:946	arg1	chloride					948:955	sodium chloride	941:955	sodium chloride	941:955	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	3	4	theme	contract	490:497	arg1	time					499:502	contract time	490:502	contract time	490:502	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	7	5	theme	wastewater	1145:1154	arg1	process					1166:1172	a wastewater treatment process	1143:1172	a wastewater treatment process	1143:1172	SBCS would be a good method and resource to increase absorption efficiency for the removal of anionic dyes in a wastewater treatment process.
26432372	3	6	theme	adsorbent	457:465	arg1	dosage					467:472	adsorbent dosage	457:472	adsorbent dosage	457:472	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	6	7	theme	potential	914:922	arg1	measurement					924:934	zeta potential measurement	909:934	zeta potential measurement	909:934	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	7	8	theme	dyes	1135:1138	arg1	removal					1116:1122	the removal	1112:1122	the removal of anionic dyes	1112:1138	SBCS would be a good method and resource to increase absorption efficiency for the removal of anionic dyes in a wastewater treatment process.
26432372	2	9	theme	red	326:328	arg1	adsorption					306:315	the adsorption	302:315	the adsorption of congo red, an anionic azo dye by GL-SBCS	302:359	Physico-chemical investigation on the adsorption of congo red, an anionic azo dye by GL-SBCS has been carried out.
26432372	3	10	theme	chitosan	426:433	arg1	contents					414:421	different weight contents	397:421	different weight contents of chitosan	397:433	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	6	11	theme	physical	823:830	arg1	forces					832:837	physical forces	823:837	physical forces	823:837	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	7	12	theme	good	1049:1052	arg1	method					1054:1059	a good method	1047:1059	a good method	1047:1059	SBCS would be a good method and resource to increase absorption efficiency for the removal of anionic dyes in a wastewater treatment process.
26432372	7	12	theme	good	1049:1052	arg1	SBCS					1033:1036	SBCS	1033:1036	SBCS	1033:1036	SBCS would be a good method and resource to increase absorption efficiency for the removal of anionic dyes in a wastewater treatment process.
26432372	7	13	theme	anionic	1127:1133	arg1	dyes					1135:1138	anionic dyes	1127:1138	anionic dyes	1127:1138	SBCS would be a good method and resource to increase absorption efficiency for the removal of anionic dyes in a wastewater treatment process.
26432372	3	14	theme	different	397:405	arg1	contents					414:421	different weight contents	397:421	different weight contents of chitosan	397:433	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	3	15	theme	initial	475:481	arg1	pH					483:484	initial pH	475:484	initial pH	475:484	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	5	16	theme	Langmuir	721:728	arg1	model					730:734	the Langmuir model	717:734	the Langmuir model (R(2)>0.99)	717:746	Further investigation demonstrated that the adsorption pattern fitted well with the Langmuir model (R(2)>0.99) but less-satisfied the Freundlich model.
26432372	5	16	theme	Langmuir	721:728	arg1	R					737:737	R(2)>0.99	737:745	R(2)>0.99	737:745	Further investigation demonstrated that the adsorption pattern fitted well with the Langmuir model (R(2)>0.99) but less-satisfied the Freundlich model.
26432372	0	17	theme	Schiff	0:5	arg1	base					7:10	Schiff base	0:10	Schiff base	0:10	Schiff base - Chitosan grafted L-monoguluronic acid as a novel solid-phase adsorbent for removal of congo red.
26432372	6	18	theme	zeta	909:912	arg1	measurement					924:934	zeta potential measurement	909:934	zeta potential measurement	909:934	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	4	19	theme	l-monoguluronic	615:629	arg1	acid					631:634	l-monoguluronic acid	615:634	l-monoguluronic acid	615:634	Results showed that GL-SBCS exhibited better than normal CS and l-monoguluronic acid.
26432372	6	20	with	binding	858:864	arg1	GL-SBCS					884:890	GL-SBCS	884:890	GL-SBCS	884:890	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	1	21	theme	l-monoguluronic	246:260	arg1	acid					262:265	l-monoguluronic acid	246:265	l-monoguluronic acid	246:265	A novel modified chitosan adsorbent (GL-SBCS) was synthesized by covalently grafting a Schiff base-chitosan (SBCS) onto the surface of l-monoguluronic acid.
26432372	5	22	theme	Freundlich	771:780	arg1	model					782:786	the Freundlich model	767:786	the Freundlich model	767:786	Further investigation demonstrated that the adsorption pattern fitted well with the Langmuir model (R(2)>0.99) but less-satisfied the Freundlich model.
26432372	1	23	theme	acid	262:265	arg1	surface					235:241	the surface	231:241	the surface of l-monoguluronic acid	231:265	A novel modified chitosan adsorbent (GL-SBCS) was synthesized by covalently grafting a Schiff base-chitosan (SBCS) onto the surface of l-monoguluronic acid.
26432372	0	24	theme	red	106:108	arg1	removal					89:95	removal	89:95	removal of congo red	89:108	Schiff base - Chitosan grafted L-monoguluronic acid as a novel solid-phase adsorbent for removal of congo red.
26432372	2	25	theme	Physico-chemical	268:283	arg1	investigation					285:297	Physico-chemical investigation	268:297	Physico-chemical investigation on the adsorption of congo red, an anionic azo dye by GL-SBCS	268:359	Physico-chemical investigation on the adsorption of congo red, an anionic azo dye by GL-SBCS has been carried out.
26432372	1	26	theme	modified	119:126	arg1	chitosan					128:135	A novel modified chitosan adsorbent	111:145	A novel modified chitosan adsorbent (GL-SBCS)	111:155	A novel modified chitosan adsorbent (GL-SBCS) was synthesized by covalently grafting a Schiff base-chitosan (SBCS) onto the surface of l-monoguluronic acid.
26432372	1	26	theme	modified	119:126	arg1	GL-SBCS					148:154	GL-SBCS	148:154	GL-SBCS	148:154	A novel modified chitosan adsorbent (GL-SBCS) was synthesized by covalently grafting a Schiff base-chitosan (SBCS) onto the surface of l-monoguluronic acid.
26432372	0	27	theme	L-monoguluronic	31:45	arg1	solid-phase					63:73	a novel solid-phase	55:73	a novel solid-phase adsorbent for removal of congo red	55:108	Schiff base - Chitosan grafted L-monoguluronic acid as a novel solid-phase adsorbent for removal of congo red.
26432372	0	27	theme	L-monoguluronic	31:45	arg1	acid					47:50	L-monoguluronic acid	31:50	L-monoguluronic acid	31:50	Schiff base - Chitosan grafted L-monoguluronic acid as a novel solid-phase adsorbent for removal of congo red.
26432372	5	28	theme	adsorption	681:690	arg1	pattern					692:698	the adsorption pattern	677:698	the adsorption pattern	677:698	Further investigation demonstrated that the adsorption pattern fitted well with the Langmuir model (R(2)>0.99) but less-satisfied the Freundlich model.
26432372	4	29	theme	normal	601:606	arg1	CS					608:609	normal CS	601:609	normal CS	601:609	Results showed that GL-SBCS exhibited better than normal CS and l-monoguluronic acid.
26432372	6	30	theme	adsorption	1013:1022	arg1	process					1024:1030	the adsorption process	1009:1030	the adsorption process	1009:1030	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	3	31	theme	weight	407:412	arg1	contents					414:421	different weight contents	397:421	different weight contents of chitosan	397:433	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	6	32	theme	dodecyl	968:974	arg1	sulfate					976:982	sodium dodecyl sulfate	961:982	sodium dodecyl sulfate	961:982	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	6	33	dep	interaction	800:810	arg1	Both					789:792	Both	789:792	Both	789:792	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	3	34	theme	contents	414:421	arg1	effect					387:392	The effect	383:392	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time	383:502	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	6	35	theme	ionic	794:798	arg1	interaction					800:810	ionic interaction	794:810	ionic interaction	794:810	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	3	36	theme	composite	446:454	arg1	GL-SBCS					438:444	GL-SBCS composite	438:454	GL-SBCS composite	438:454	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	7	37	theme	absorption	1086:1095	arg1	efficiency					1097:1106	absorption efficiency	1086:1106	absorption efficiency for the removal of anionic dyes	1086:1138	SBCS would be a good method and resource to increase absorption efficiency for the removal of anionic dyes in a wastewater treatment process.
26432372	6	38	theme	red	875:877	arg1	binding					858:864	binding	858:864	binding of congo red with GL-SBCS	858:890	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	5	39	theme	Further	637:643	arg1	investigation					645:657	Further investigation	637:657	Further investigation	637:657	Further investigation demonstrated that the adsorption pattern fitted well with the Langmuir model (R(2)>0.99) but less-satisfied the Freundlich model.
26432372	3	40	theme	batch	533:537	arg1	adsorption					539:548	batch adsorption	533:548	batch adsorption	533:548	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	1	41	theme	Schiff	198:203	arg1	SBCS					220:223	SBCS	220:223	SBCS	220:223	A novel modified chitosan adsorbent (GL-SBCS) was synthesized by covalently grafting a Schiff base-chitosan (SBCS) onto the surface of l-monoguluronic acid.
26432372	1	41	theme	Schiff	198:203	arg1	base-chitosan					205:217	a Schiff base-chitosan	196:217	a Schiff base-chitosan (SBCS)	196:224	A novel modified chitosan adsorbent (GL-SBCS) was synthesized by covalently grafting a Schiff base-chitosan (SBCS) onto the surface of l-monoguluronic acid.
26432372	1	42	theme	novel	113:117	arg1	chitosan					128:135	A novel modified chitosan adsorbent	111:145	A novel modified chitosan adsorbent (GL-SBCS)	111:155	A novel modified chitosan adsorbent (GL-SBCS) was synthesized by covalently grafting a Schiff base-chitosan (SBCS) onto the surface of l-monoguluronic acid.
26432372	1	42	theme	novel	113:117	arg1	GL-SBCS					148:154	GL-SBCS	148:154	GL-SBCS	148:154	A novel modified chitosan adsorbent (GL-SBCS) was synthesized by covalently grafting a Schiff base-chitosan (SBCS) onto the surface of l-monoguluronic acid.
26432372	6	43	theme	sodium	961:966	arg1	sulfate					976:982	sodium dodecyl sulfate	961:982	sodium dodecyl sulfate	961:982	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	0	44	theme	novel	57:61	arg1	solid-phase					63:73	a novel solid-phase	55:73	a novel solid-phase adsorbent for removal of congo red	55:108	Schiff base - Chitosan grafted L-monoguluronic acid as a novel solid-phase adsorbent for removal of congo red.
26432372	0	44	theme	novel	57:61	arg1	acid					47:50	L-monoguluronic acid	31:50	L-monoguluronic acid	31:50	Schiff base - Chitosan grafted L-monoguluronic acid as a novel solid-phase adsorbent for removal of congo red.
26432372	3	45	from	effect	387:392	arg1	dosage					467:472	adsorbent dosage	457:472	adsorbent dosage	457:472	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	3	45	from	effect	387:392	arg1	pH					483:484	initial pH	475:484	initial pH	475:484	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	3	45	from	effect	387:392	arg1	time					499:502	contract time	490:502	contract time	490:502	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	3	45	from	effect	387:392	arg1	GL-SBCS					438:444	GL-SBCS composite	438:454	GL-SBCS composite	438:454	The effect of different weight contents of chitosan in GL-SBCS composite, adsorbent dosage, initial pH and contract time were studied in detail using batch adsorption.
26432372	2	46	theme	azo	342:344	arg1	dye					346:348	an anionic azo dye	331:348	an anionic azo dye	331:348	Physico-chemical investigation on the adsorption of congo red, an anionic azo dye by GL-SBCS has been carried out.
26432372	2	46	theme	azo	342:344	arg1	red					326:328	red	326:328	red	326:328	Physico-chemical investigation on the adsorption of congo red, an anionic azo dye by GL-SBCS has been carried out.
26432372	6	47	dep	measurement	924:934	arg1	Both					936:939	Both	936:939	Both	936:939	Both ionic interaction as well as physical forces is responsible for binding of congo red with GL-SBCS as determined by zeta potential measurement Both sodium chloride and sodium dodecyl sulfate significantly influenced the adsorption process.
26432372	2	48	theme	anionic	334:340	arg1	dye					346:348	an anionic azo dye	331:348	an anionic azo dye	331:348	Physico-chemical investigation on the adsorption of congo red, an anionic azo dye by GL-SBCS has been carried out.
26432372	2	48	theme	anionic	334:340	arg1	red					326:328	red	326:328	red	326:328	Physico-chemical investigation on the adsorption of congo red, an anionic azo dye by GL-SBCS has been carried out.
26432372	2	49	from	investigation	285:297	arg1	adsorption					306:315	the adsorption	302:315	the adsorption of congo red, an anionic azo dye by GL-SBCS	302:359	Physico-chemical investigation on the adsorption of congo red, an anionic azo dye by GL-SBCS has been carried out.
26488434	6	0	theme	fingerprint	883:893	arg1	region					904:909	the fingerprint spectral region	879:909	the fingerprint spectral region	879:909	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	6	1	theme	non-Saccharomyces	968:984	arg1	species					986:992	non-Saccharomyces species	968:992	non-Saccharomyces species	968:992	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	7	2	theme	loading	1077:1083	arg1	plots					1085:1089	PCA loading plots	1073:1089	PCA loading plots	1073:1089	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	5	3	dep	stationery	810:819	arg1	must					802:805	must	802:805	must	802:805	Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum.
26488434	3	4	theme	mid-infrared	510:521	arg1	spectroscopy					529:540	mid-infrared (MIR) spectroscopy	510:540	mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA)	510:598	To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA).
26488434	1	5	theme	glucan	264:269	arg1	polymers					271:278	glucan polymers	264:278	glucan polymers	264:278	Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers.
26488434	7	6	theme	wall	1189:1192	arg1	proteins					1194:1201	cell wall proteins	1184:1201	cell wall proteins (e.g., mannoproteins)	1184:1223	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	7	6	theme	wall	1189:1192	arg1	mannoproteins					1210:1222	mannoproteins	1210:1222	mannoproteins	1210:1222	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	3	7	from	effect	428:433	arg1	composition					469:479	cell wall composition	459:479	cell wall composition	459:479	To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA).
26488434	1	8	gly	glycoproteins	236:248	arg1	glycoproteins					236:248	glycoproteins	236:248	glycoproteins	236:248	Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers.
26488434	2	9	theme	relevant	306:313	arg1	phenotypes					295:304	Several yeast phenotypes	281:304	Several yeast phenotypes relevant for fermentation	281:330	Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties.
26488434	0	10	theme	surface	100:106	arg1	mannoproteins					108:120	the surface mannoproteins	96:120	the surface mannoproteins	96:120	A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations.
26488434	7	11	theme	OPLS-DA	1106:1112	arg1	plots					1085:1089	PCA loading plots	1073:1089	PCA loading plots	1073:1089	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	7	11	theme	OPLS-DA	1106:1112	arg1	use					1099:1101	the use	1095:1101	the use of OPLS-DA to the data sets	1095:1129	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	4	12	theme	strains	621:627	arg1	total					603:607	A total	601:607	A total of 40 yeast strains	601:627	A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species.
26488434	6	13	from	species	986:992	arg1	separation					927:936	distinct separation	918:936	distinct separation of Saccharomyces strains from non-Saccharomyces species	918:992	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	2	14	theme	yeast	289:293	arg1	phenotypes					295:304	Several yeast phenotypes	281:304	Several yeast phenotypes relevant for fermentation	281:330	Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties.
26488434	1	15	theme	Yeast	183:187	arg1	cells					189:193	Yeast cells	183:193	Yeast cells	183:193	Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers.
26488434	5	16	theme	Chardonnay	785:794	arg1	grape					796:800	Chardonnay grape	785:800	Chardonnay grape	785:800	Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum.
26488434	5	17	theme	synthetic	765:773	arg1	MS300					775:779	synthetic MS300	765:779	synthetic MS300	765:779	Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum.
26488434	2	18	theme	Several	281:287	arg1	phenotypes					295:304	Several yeast phenotypes	281:304	Several yeast phenotypes relevant for fermentation	281:330	Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties.
26488434	3	19	dep	PCA	583:585	arg1	i.e.					577:580	i.e.	577:580	i.e.	577:580	To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA).
26488434	0	20	theme	multivariate	2:13	arg1	approach					15:22	A multivariate approach	0:22	A multivariate approach	0:22	A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations.
26488434	7	21	theme	laboratory	1234:1243	arg1	strains					1245:1251	laboratory strains	1234:1251	laboratory strains	1234:1251	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	2	22	theme	wine	333:336	arg1	processing					338:347	wine processing	333:347	wine processing	333:347	Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties.
26488434	2	23	theme	wall	392:395	arg1	properties					397:406	cell wall properties	387:406	cell wall properties	387:406	Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties.
26488434	4	24	theme	non-Saccharomyces	709:725	arg1	species					727:733	non-Saccharomyces species	709:733	non-Saccharomyces species	709:733	A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species.
26488434	7	25	theme	PCA	1073:1075	arg1	plots					1085:1089	PCA loading plots	1073:1089	PCA loading plots	1073:1089	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	2	26	theme	cell	387:390	arg1	properties					397:406	cell wall properties	387:406	cell wall properties	387:406	Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties.
26488434	7	27	theme	industrial	1146:1155	arg1	strains					1157:1163	industrial strains	1146:1163	industrial strains	1146:1163	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	6	28	theme	strains	955:961	arg1	separation					927:936	distinct separation	918:936	distinct separation of Saccharomyces strains from non-Saccharomyces species	918:992	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	0	29	theme	yeast	139:143	arg1	walls					150:154	yeast cell walls	139:154	yeast cell walls	139:154	A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations.
26488434	3	30	theme	multivariate	555:566	arg1	methods					568:574	multivariate methods	555:574	multivariate methods (i.e., PCA and OPLS-DA)	555:598	To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA).
26488434	1	31	theme	cell	205:208	arg1	wall					210:213	a cell wall	203:213	a cell wall comprising primarily glycoproteins, mannans, and glucan polymers	203:278	Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers.
26488434	6	32	theme	Saccharomyces	941:953	arg1	strains					955:961	Saccharomyces strains	941:961	Saccharomyces strains	941:961	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	4	33	dep	strains	669:675	arg1	industrial					693:702	industrial	693:702	industrial	693:702	A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species.
26488434	4	33	dep	strains	669:675	arg1	laboratory					678:687	laboratory	678:687	laboratory	678:687	A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species.
26488434	7	34	theme	glucan	1288:1293	arg1	polymers					1295:1302	mannan and glucan polymers	1277:1302	mannan and glucan polymers	1277:1302	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	0	35	theme	total	41:45	arg1	spectroscopy					72:83	attenuated total reflectance mid-infrared spectroscopy	30:83	attenuated total reflectance mid-infrared spectroscopy	30:83	A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations.
26488434	7	36	theme	mannan	1277:1282	arg1	polymers					1295:1302	mannan and glucan polymers	1277:1302	mannan and glucan polymers	1277:1302	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	0	37	theme	walls	150:154	arg1	mannoproteins					108:120	the surface mannoproteins	96:120	the surface mannoproteins	96:120	A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations.
26488434	0	37	theme	walls	150:154	arg1	β-glucans					126:134	β-glucans	126:134	β-glucans	126:134	A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations.
26488434	1	38	contain	possess	195:201	arg2	wall					210:213	a cell wall	203:213	a cell wall comprising primarily glycoproteins, mannans, and glucan polymers	203:278	Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers.
26488434	1	38	contain	possess	195:201	arg1	cells					189:193	Yeast cells	183:193	Yeast cells	183:193	Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers.
26488434	3	39	theme	wine	438:441	arg1	fermentation					443:454	wine fermentation	438:454	wine fermentation	438:454	To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA).
26488434	3	40	theme	cell	459:462	arg1	composition					469:479	cell wall composition	459:479	cell wall composition	459:479	To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA).
26488434	0	41	theme	attenuated	30:39	arg1	spectroscopy					72:83	attenuated total reflectance mid-infrared spectroscopy	30:83	attenuated total reflectance mid-infrared spectroscopy	30:83	A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations.
26488434	0	42	theme	cell	145:148	arg1	walls					150:154	yeast cell walls	139:154	yeast cell walls	139:154	A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations.
26488434	3	43	theme	wall	464:467	arg1	composition					469:479	cell wall composition	459:479	cell wall composition	459:479	To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA).
26488434	0	44	theme	mid-infrared	59:70	arg1	spectroscopy					72:83	attenuated total reflectance mid-infrared spectroscopy	30:83	attenuated total reflectance mid-infrared spectroscopy	30:83	A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations.
26488434	6	45	theme	distinct	918:925	arg1	separation					927:936	distinct separation	918:936	distinct separation of Saccharomyces strains from non-Saccharomyces species	918:992	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	0	46	theme	wine	163:166	arg1	fermentations					168:180	wine fermentations	163:180	wine fermentations	163:180	A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations.
26488434	3	47	theme	fermentation	443:454	arg1	effect					428:433	the effect	424:433	the effect of wine fermentation on cell wall composition	424:479	To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA).
26488434	2	48	theme	wine	354:357	arg1	quality					359:365	wine quality	354:365	wine quality	354:365	Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties.
26488434	0	49	theme	reflectance	47:57	arg1	spectroscopy					72:83	attenuated total reflectance mid-infrared spectroscopy	30:83	attenuated total reflectance mid-infrared spectroscopy	30:83	A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations.
26488434	7	50	theme	cell	1184:1187	arg1	proteins					1194:1201	cell wall proteins	1184:1201	cell wall proteins (e.g., mannoproteins)	1184:1223	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	7	50	theme	cell	1184:1187	arg1	mannoproteins					1210:1222	mannoproteins	1210:1222	mannoproteins	1210:1222	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	6	51	theme	region	904:909	arg1	PCA					872:874	PCA	872:874	PCA of the fingerprint spectral region	872:909	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	7	52	dep	enriched	1170:1177	arg1	whereas					1226:1232	whereas	1226:1232	whereas	1226:1232	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	7	53	dep	mannoproteins	1210:1222	arg1	e.g.					1204:1207	e.g.	1204:1207	e.g.	1204:1207	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	6	54	theme	yeast	1024:1028	arg1	strains					1030:1036	industrial wine yeast strains	1008:1036	industrial wine yeast strains	1008:1036	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	4	55	theme	Saccharomyces	655:667	arg1	strains					669:675	Saccharomyces strains	655:675	Saccharomyces strains (laboratory and industrial)	655:703	A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species.
26488434	6	56	theme	wine	1019:1022	arg1	strains					1030:1036	industrial wine yeast strains	1008:1036	industrial wine yeast strains	1008:1036	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	4	57	theme	yeast	615:619	arg1	strains					621:627	40 yeast strains	612:627	40 yeast strains	612:627	A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species.
26488434	6	58	theme	laboratory	1053:1062	arg1	strains					1064:1070	laboratory strains	1053:1070	laboratory strains	1053:1070	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	5	59	theme	MIR	858:860	arg1	spectrum					862:869	the MIR spectrum	854:869	the MIR spectrum	854:869	Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum.
26488434	6	60	theme	industrial	1008:1017	arg1	strains					1030:1036	industrial wine yeast strains	1008:1036	industrial wine yeast strains	1008:1036	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	7	61	theme	data	1121:1124	arg1	sets					1126:1129	the data sets	1117:1129	the data sets	1117:1129	PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.
26488434	6	62	theme	spectral	895:902	arg1	region					904:909	the fingerprint spectral region	879:909	the fingerprint spectral region	879:909	PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains.
26488434	3	63	theme	MIR	524:526	arg1	spectroscopy					529:540	mid-infrared (MIR) spectroscopy	510:540	mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA)	510:598	To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA).
27987936	0	0	theme	sulfonate	90:98	arg1	gels					100:103	carboxymethyl cellulose/sodium styrene sulfonate gels	51:103	carboxymethyl cellulose/sodium styrene sulfonate gels	51:103	Removal of metal ions from aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels prepared by radiation grafting.
27987936	9	1	theme	Langmuir	1142:1149	arg1	model					1151:1155	Langmuir model	1142:1155	Langmuir model	1142:1155	The adsorption kinetics and equilibrium isotherms were investigated using pseudo-second-order model and Langmuir model.
27987936	1	2	theme	CMCNa	169:173	arg1	Sulfonate					191:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate	137:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks	137:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	1	2	theme	CMCNa	169:173	arg1	SSS					202:204	SSS	202:204	SSS	202:204	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	4	3	theme	SSS	574:576	arg1	content					578:584	SSS content	574:584	SSS content	574:584	The swelling capacity increased by increasing SSS content due to the presence of SO3Na, OH groups in gel structure.
27987936	0	4	theme	styrene	82:88	arg1	gels					100:103	carboxymethyl cellulose/sodium styrene sulfonate gels	51:103	carboxymethyl cellulose/sodium styrene sulfonate gels	51:103	Removal of metal ions from aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels prepared by radiation grafting.
27987936	6	5	theme	adsorption	772:781	arg1	capacity					783:790	The metal ion adsorption capacity	758:790	The metal ion adsorption capacity of CMC/SSS gel	758:805	The metal ion adsorption capacity of CMC/SSS gel was investigated.
27987936	7	6	theme	grafted	829:835	arg1	gel					837:839	The grafted gel	825:839	The grafted gel	825:839	The grafted gel effectively removed metal ions, especially Cr and Pb.
27987936	5	7	theme	new	688:690	arg1	peaks					703:707	the new absorption peaks	684:707	the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group	684:755	The FTIR spectrum of CMC/SSS gel showed the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group.
27987936	6	8	theme	ion	768:770	arg1	capacity					783:790	The metal ion adsorption capacity	758:790	The metal ion adsorption capacity of CMC/SSS gel	758:805	The metal ion adsorption capacity of CMC/SSS gel was investigated.
27987936	4	9	theme	gel	629:631	arg1	structure					633:641	gel structure	629:641	gel structure	629:641	The swelling capacity increased by increasing SSS content due to the presence of SO3Na, OH groups in gel structure.
27987936	5	10	theme	absorption	692:701	arg1	peaks					703:707	the new absorption peaks	684:707	the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group	684:755	The FTIR spectrum of CMC/SSS gel showed the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group.
27987936	8	11	theme	adsorption	979:988	arg1	capacity					990:997	the adsorption capacity	975:997	the adsorption capacity of the grafted hydrogels	975:1022	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels were studied.
27987936	6	12	theme	metal	762:766	arg1	capacity					783:790	The metal ion adsorption capacity	758:790	The metal ion adsorption capacity of CMC/SSS gel	758:805	The metal ion adsorption capacity of CMC/SSS gel was investigated.
27987936	0	13	theme	radiation	117:125	arg1	grafting					127:134	radiation grafting	117:134	radiation grafting	117:134	Removal of metal ions from aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels prepared by radiation grafting.
27987936	0	14	from	solutions	35:43	arg1	Removal					0:6	Removal	0:6	Removal of metal ions from aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels	0:103	Removal of metal ions from aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels prepared by radiation grafting.
27987936	8	15	theme	contact	932:938	arg1	time					940:943	contact time	932:943	contact time	932:943	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels were studied.
27987936	3	16	theme	other	514:518	arg1	ratios					520:525	other ratios	514:525	other ratios	514:525	The results showed the ratio of CMC and SSS 1:0 gave the highest gel fraction, compared with other ratios.
27987936	1	17	theme	/Sodium	175:181	arg1	Sulfonate					191:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate	137:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks	137:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	1	17	theme	/Sodium	175:181	arg1	SSS					202:204	SSS	202:204	SSS	202:204	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	1	18	link	crosslinked	234:244	arg1	networks					256:263	grafted and crosslinked polymeric networks	222:263	grafted and crosslinked polymeric networks	222:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	3	19	theme	highest	478:484	arg1	fraction					490:497	the highest gel fraction	474:497	the highest gel fraction	474:497	The results showed the ratio of CMC and SSS 1:0 gave the highest gel fraction, compared with other ratios.
27987936	1	20	theme	Styrene	183:189	arg1	Sulfonate					191:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate	137:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks	137:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	1	20	theme	Styrene	183:189	arg1	SSS					202:204	SSS	202:204	SSS	202:204	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	8	21	theme	hydrogels	1014:1022	arg1	capacity					990:997	the adsorption capacity	975:997	the adsorption capacity of the grafted hydrogels	975:1022	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels were studied.
27987936	1	22	theme	Sulfonate	191:199	arg1	hydrogels					207:215	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels	137:215	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks	137:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	8	23	theme	grafted	1006:1012	arg1	hydrogels					1014:1022	the grafted hydrogels	1002:1022	the grafted hydrogels	1002:1022	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels were studied.
27987936	8	24	theme	concentration	958:970	arg1	effects					899:905	The effects	895:905	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels	895:1022	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels were studied.
27987936	2	25	theme	obtained	323:330	arg1	hydrogels					332:340	The obtained hydrogels	319:340	The obtained hydrogels	319:340	The obtained hydrogels were characterized by gel fraction, swelling ratio, TGA and FTIR spectroscopy.
27987936	4	26	from	groups	619:624	arg1	structure					633:641	gel structure	629:641	gel structure	629:641	The swelling capacity increased by increasing SSS content due to the presence of SO3Na, OH groups in gel structure.
27987936	0	27	theme	ions	17:20	arg1	Removal					0:6	Removal	0:6	Removal of metal ions from aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels	0:103	Removal of metal ions from aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels prepared by radiation grafting.
27987936	4	28	theme	swelling	532:539	arg1	capacity					541:548	The swelling capacity	528:548	The swelling capacity	528:548	The swelling capacity increased by increasing SSS content due to the presence of SO3Na, OH groups in gel structure.
27987936	1	29	with	hydrogels	207:215	arg1	networks					256:263	grafted and crosslinked polymeric networks	222:263	grafted and crosslinked polymeric networks	222:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	8	30	theme	initial	950:956	arg1	concentration					958:970	initial concentration	950:970	initial concentration	950:970	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels were studied.
27987936	9	31	theme	kinetics	1053:1060	arg1	isotherms					1078:1086	The adsorption kinetics and equilibrium isotherms	1038:1086	The adsorption kinetics and equilibrium isotherms	1038:1086	The adsorption kinetics and equilibrium isotherms were investigated using pseudo-second-order model and Langmuir model.
27987936	3	32	theme	SSS	461:463	arg1	ratio					444:448	the ratio	440:448	the ratio of CMC and SSS 1:0	440:467	The results showed the ratio of CMC and SSS 1:0 gave the highest gel fraction, compared with other ratios.
27987936	0	33	theme	metal	11:15	arg1	ions					17:20	metal ions	11:20	metal ions	11:20	Removal of metal ions from aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels prepared by radiation grafting.
27987936	1	34	theme	atmosphere	297:306	arg1	condition					308:316	atmosphere condition	297:316	atmosphere condition	297:316	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	5	35	theme	gel	673:675	arg1	spectrum					653:660	The FTIR spectrum	644:660	The FTIR spectrum of CMC/SSS gel	644:675	The FTIR spectrum of CMC/SSS gel showed the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group.
27987936	6	36	theme	gel	803:805	arg1	capacity					783:790	The metal ion adsorption capacity	758:790	The metal ion adsorption capacity of CMC/SSS gel	758:805	The metal ion adsorption capacity of CMC/SSS gel was investigated.
27987936	4	37	theme	SO3Na	609:613	arg1	presence					597:604	the presence	593:604	the presence of SO3Na, OH groups in gel structure	593:641	The swelling capacity increased by increasing SSS content due to the presence of SO3Na, OH groups in gel structure.
27987936	0	38	theme	aqueous	27:33	arg1	solutions					35:43	aqueous solutions	27:43	aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels	27:103	Removal of metal ions from aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels prepared by radiation grafting.
27987936	5	39	dep	1034	712:715	arg1	corresponds					730:740	corresponds	730:740	corresponds to SO3Na group	730:755	The FTIR spectrum of CMC/SSS gel showed the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group.
27987936	8	40	from	effects	899:905	arg1	capacity					990:997	the adsorption capacity	975:997	the adsorption capacity of the grafted hydrogels	975:1022	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels were studied.
27987936	3	41	dep	showed	433:438	arg1	gave					469:472	gave	469:472	gave the highest gel fraction	469:497	The results showed the ratio of CMC and SSS 1:0 gave the highest gel fraction, compared with other ratios.
27987936	9	42	theme	equilibrium	1066:1076	arg1	isotherms					1078:1086	The adsorption kinetics and equilibrium isotherms	1038:1086	The adsorption kinetics and equilibrium isotherms	1038:1086	The adsorption kinetics and equilibrium isotherms were investigated using pseudo-second-order model and Langmuir model.
27987936	3	43	theme	CMC	453:455	arg1	ratio					444:448	the ratio	440:448	the ratio of CMC and SSS 1:0	440:467	The results showed the ratio of CMC and SSS 1:0 gave the highest gel fraction, compared with other ratios.
27987936	2	44	theme	FTIR	402:405	arg1	spectroscopy					407:418	FTIR spectroscopy	402:418	FTIR spectroscopy	402:418	The obtained hydrogels were characterized by gel fraction, swelling ratio, TGA and FTIR spectroscopy.
27987936	8	45	theme	time	940:943	arg1	effects					899:905	The effects	895:905	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels	895:1022	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels were studied.
27987936	5	46	theme	SO3Na	745:749	arg1	group					751:755	SO3Na group	745:755	SO3Na group	745:755	The FTIR spectrum of CMC/SSS gel showed the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group.
27987936	9	47	theme	adsorption	1042:1051	arg1	kinetics					1053:1060	adsorption kinetics	1042:1060	adsorption kinetics	1042:1060	The adsorption kinetics and equilibrium isotherms were investigated using pseudo-second-order model and Langmuir model.
27987936	4	48	theme	OH	616:617	arg1	SO3Na					609:613	SO3Na	609:613	SO3Na	609:613	The swelling capacity increased by increasing SSS content due to the presence of SO3Na, OH groups in gel structure.
27987936	4	48	theme	OH	616:617	arg1	groups					619:624	OH groups	616:624	OH groups in gel structure	616:641	The swelling capacity increased by increasing SSS content due to the presence of SO3Na, OH groups in gel structure.
27987936	2	49	theme	gel	364:366	arg1	fraction					368:375	gel fraction	364:375	gel fraction	364:375	The obtained hydrogels were characterized by gel fraction, swelling ratio, TGA and FTIR spectroscopy.
27987936	5	50	theme	FTIR	648:651	arg1	spectrum					653:660	The FTIR spectrum	644:660	The FTIR spectrum of CMC/SSS gel	644:675	The FTIR spectrum of CMC/SSS gel showed the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group.
27987936	5	51	from	1012cm-1	721:728	arg1	peaks					703:707	the new absorption peaks	684:707	the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group	684:755	The FTIR spectrum of CMC/SSS gel showed the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group.
27987936	3	52	theme	gel	486:488	arg1	fraction					490:497	the highest gel fraction	474:497	the highest gel fraction	474:497	The results showed the ratio of CMC and SSS 1:0 gave the highest gel fraction, compared with other ratios.
27987936	6	53	theme	CMC/SSS	795:801	arg1	gel					803:805	CMC/SSS gel	795:805	CMC/SSS gel	795:805	The metal ion adsorption capacity of CMC/SSS gel was investigated.
27987936	3	54	dep	CMC	453:455	arg1	1:0					465:467	1:0	465:467	1:0	465:467	The results showed the ratio of CMC and SSS 1:0 gave the highest gel fraction, compared with other ratios.
27987936	8	55	theme	composition	919:929	arg1	effects					899:905	The effects	895:905	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels	895:1022	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels were studied.
27987936	1	56	theme	Sodium	137:142	arg1	Sulfonate					191:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate	137:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks	137:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	1	56	theme	Sodium	137:142	arg1	SSS					202:204	SSS	202:204	SSS	202:204	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	1	57	theme	grafted	222:228	arg1	networks					256:263	grafted and crosslinked polymeric networks	222:263	grafted and crosslinked polymeric networks	222:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	5	58	from	1034	712:715	arg1	peaks					703:707	the new absorption peaks	684:707	the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group	684:755	The FTIR spectrum of CMC/SSS gel showed the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group.
27987936	9	59	theme	pseudo-second-order	1112:1130	arg1	model					1132:1136	pseudo-second-order model	1112:1136	pseudo-second-order model	1112:1136	The adsorption kinetics and equilibrium isotherms were investigated using pseudo-second-order model and Langmuir model.
27987936	1	60	theme	Carboxymethyl	144:156	arg1	Sulfonate					191:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate	137:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks	137:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	1	60	theme	Carboxymethyl	144:156	arg1	SSS					202:204	SSS	202:204	SSS	202:204	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	7	61	theme	metal	861:865	arg1	Pb					891:892	Pb	891:892	Pb	891:892	The grafted gel effectively removed metal ions, especially Cr and Pb.
27987936	7	61	theme	metal	861:865	arg1	ions					867:870	metal ions	861:870	metal ions	861:870	The grafted gel effectively removed metal ions, especially Cr and Pb.
27987936	7	61	theme	metal	861:865	arg1	Cr					884:885	Cr	884:885	Cr	884:885	The grafted gel effectively removed metal ions, especially Cr and Pb.
27987936	0	62	theme	cellulose/sodium	65:80	arg1	gels					100:103	carboxymethyl cellulose/sodium styrene sulfonate gels	51:103	carboxymethyl cellulose/sodium styrene sulfonate gels	51:103	Removal of metal ions from aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels prepared by radiation grafting.
27987936	1	63	theme	Cellulose	158:166	arg1	Sulfonate					191:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate	137:199	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks	137:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	1	63	theme	Cellulose	158:166	arg1	SSS					202:204	SSS	202:204	SSS	202:204	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	1	64	theme	crosslinked	234:244	arg1	networks					256:263	grafted and crosslinked polymeric networks	222:263	grafted and crosslinked polymeric networks	222:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27987936	0	65	theme	carboxymethyl	51:63	arg1	gels					100:103	carboxymethyl cellulose/sodium styrene sulfonate gels	51:103	carboxymethyl cellulose/sodium styrene sulfonate gels	51:103	Removal of metal ions from aqueous solutions using carboxymethyl cellulose/sodium styrene sulfonate gels prepared by radiation grafting.
27987936	5	66	theme	CMC/SSS	665:671	arg1	gel					673:675	CMC/SSS gel	665:675	CMC/SSS gel	665:675	The FTIR spectrum of CMC/SSS gel showed the new absorption peaks at 1034 and 1012cm-1 corresponds to SO3Na group.
27987936	8	67	theme	hydrogel	910:917	arg1	composition					919:929	hydrogel composition	910:929	hydrogel composition	910:929	The effects of hydrogel composition, contact time, and initial concentration on the adsorption capacity of the grafted hydrogels were studied.
27987936	2	68	theme	swelling	378:385	arg1	ratio					387:391	swelling ratio	378:391	swelling ratio	378:391	The obtained hydrogels were characterized by gel fraction, swelling ratio, TGA and FTIR spectroscopy.
27987936	1	69	theme	polymeric	246:254	arg1	networks					256:263	grafted and crosslinked polymeric networks	222:263	grafted and crosslinked polymeric networks	222:263	Sodium Carboxymethyl Cellulose (CMCNa)/Sodium Styrene Sulfonate (SSS) hydrogels with grafted and crosslinked polymeric networks were prepared by γ-radiation at atmosphere condition.
27559395	8	0	theme	oligosaccharides	1136:1151	arg1	AGA					1118:1120	AGA	1118:1120	AGA of many other oligosaccharides	1118:1151	This process improvement is applicable to AGA of many other oligosaccharides.
27559395	8	1	theme	many	1125:1128	arg1	oligosaccharides					1136:1151	many other oligosaccharides	1125:1151	many other oligosaccharides	1125:1151	This process improvement is applicable to AGA of many other oligosaccharides.
27559395	5	2	theme	first	631:635	arg1	AGA					674:676	AGA	674:676	AGA	674:676	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	5	2	theme	first	631:635	arg1	assembly					664:671	the first iterative automated glycan assembly	627:671	the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	627:743	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	1	3	theme	bacterial	127:135	arg1	infections					137:146	the most prevalent bacterial infections	108:146	the most prevalent bacterial infections causing severe disease	108:169	Vaccines against S. pneumoniae, one of the most prevalent bacterial infections causing severe disease, rely on isolated capsular polysaccharide (CPS) that are conjugated to proteins.
27559395	5	4	theme	CPS	727:729	arg1	trisaccharide					731:743	a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	682:743	a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	682:743	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	4	5	theme	building	567:574	arg1	blocks					576:581	disaccharide building blocks	554:581	disaccharide building blocks which limits variability	554:606	Known syntheses of S. pneumoniae serotype 3 CPS rely on a time-consuming and low-yielding late-stage oxidation step, or use disaccharide building blocks which limits variability.
27559395	8	6	theme	other	1130:1134	arg1	oligosaccharides					1136:1151	many other oligosaccharides	1125:1151	many other oligosaccharides	1125:1151	This process improvement is applicable to AGA of many other oligosaccharides.
27559395	4	7	theme	time-consuming	488:501	arg1	step					541:544	a time-consuming and low-yielding late-stage oxidation step	486:544	a time-consuming and low-yielding late-stage oxidation step	486:544	Known syntheses of S. pneumoniae serotype 3 CPS rely on a time-consuming and low-yielding late-stage oxidation step, or use disaccharide building blocks which limits variability.
27559395	5	8	theme	iterative	637:645	arg1	AGA					674:676	AGA	674:676	AGA	674:676	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	5	8	theme	iterative	637:645	arg1	assembly					664:671	the first iterative automated glycan assembly	627:671	the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	627:743	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	6	9	theme	building	811:818	arg1	block					820:824	a novel glucuronic acid building block	787:824	a novel glucuronic acid building block to circumvent the need for a late-stage oxidation	787:874	This oligosaccharide was assembled using a novel glucuronic acid building block to circumvent the need for a late-stage oxidation.
27559395	2	10	theme	lengths	333:339	arg1	mixture					306:312	a heterogeneous oligosaccharide mixture	274:312	a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts	274:356	Such isolates contain a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts.
27559395	4	11	theme	disaccharide	554:565	arg1	blocks					576:581	disaccharide building blocks	554:581	disaccharide building blocks which limits variability	554:606	Known syntheses of S. pneumoniae serotype 3 CPS rely on a time-consuming and low-yielding late-stage oxidation step, or use disaccharide building blocks which limits variability.
27559395	5	12	theme	automated	647:655	arg1	AGA					674:676	AGA	674:676	AGA	674:676	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	5	12	theme	automated	647:655	arg1	assembly					664:671	the first iterative automated glycan assembly	627:671	the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	627:743	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	6	13	theme	acid	806:809	arg1	block					820:824	a novel glucuronic acid building block	787:824	a novel glucuronic acid building block to circumvent the need for a late-stage oxidation	787:874	This oligosaccharide was assembled using a novel glucuronic acid building block to circumvent the need for a late-stage oxidation.
27559395	5	14	theme	serotype	716:723	arg1	trisaccharide					731:743	a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	682:743	a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	682:743	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	4	15	theme	serotype	463:470	arg1	syntheses					436:444	Known syntheses	430:444	Known syntheses of S. pneumoniae serotype 3 CPS	430:476	Known syntheses of S. pneumoniae serotype 3 CPS rely on a time-consuming and low-yielding late-stage oxidation step, or use disaccharide building blocks which limits variability.
27559395	5	16	theme	glycan	657:662	arg1	AGA					674:676	AGA	674:676	AGA	674:676	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	5	16	theme	glycan	657:662	arg1	assembly					664:671	the first iterative automated glycan assembly	627:671	the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	627:743	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	6	17	theme	glucuronic	795:804	arg1	block					820:824	a novel glucuronic acid building block	787:824	a novel glucuronic acid building block to circumvent the need for a late-stage oxidation	787:874	This oligosaccharide was assembled using a novel glucuronic acid building block to circumvent the need for a late-stage oxidation.
27559395	2	18	theme	Such	252:255	arg1	isolates					257:264	Such isolates	252:264	Such isolates	252:264	Such isolates contain a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts.
27559395	4	19	theme	pneumoniae	452:461	arg1	serotype					463:470	S. pneumoniae serotype 3 CPS	449:476	S. pneumoniae serotype 3 CPS	449:476	Known syntheses of S. pneumoniae serotype 3 CPS rely on a time-consuming and low-yielding late-stage oxidation step, or use disaccharide building blocks which limits variability.
27559395	6	20	theme	novel	789:793	arg1	block					820:824	a novel glucuronic acid building block	787:824	a novel glucuronic acid building block to circumvent the need for a late-stage oxidation	787:874	This oligosaccharide was assembled using a novel glucuronic acid building block to circumvent the need for a late-stage oxidation.
27559395	3	21	theme	S.	387:388	arg1	structures					405:414	defined synthetic S. pneumoniae CPS structures	369:414	defined synthetic S. pneumoniae CPS structures	369:414	Access to defined synthetic S. pneumoniae CPS structures is desirable.
27559395	3	22	dep	S.	387:388	arg1	pneumoniae					390:399	pneumoniae	390:399	pneumoniae	390:399	Access to defined synthetic S. pneumoniae CPS structures is desirable.
27559395	0	23	theme	Automated	0:8	arg1	assembly					17:24	Automated glycan assembly	0:24	Automated glycan assembly of a S. pneumoniae serotype 3	0:54	Automated glycan assembly of a S. pneumoniae serotype 3 CPS antigen.
27559395	1	24	theme	severe	156:161	arg1	disease					163:169	severe disease	156:169	severe disease	156:169	Vaccines against S. pneumoniae, one of the most prevalent bacterial infections causing severe disease, rely on isolated capsular polysaccharide (CPS) that are conjugated to proteins.
27559395	0	25	theme	glycan	10:15	arg1	assembly					17:24	Automated glycan assembly	0:24	Automated glycan assembly of a S. pneumoniae serotype 3	0:54	Automated glycan assembly of a S. pneumoniae serotype 3 CPS antigen.
27559395	7	26	with	step	907:910	arg1	activator					921:929	the activator	917:929	the activator prior to each glycosylation cycle	917:963	The introduction of a washing step with the activator prior to each glycosylation cycle greatly increased the yields by neutralizing any residual base from deprotection steps in the synthetic cycle.
27559395	7	27	theme	prior	931:935	arg1	activator					921:929	the activator	917:929	the activator prior to each glycosylation cycle	917:963	The introduction of a washing step with the activator prior to each glycosylation cycle greatly increased the yields by neutralizing any residual base from deprotection steps in the synthetic cycle.
27559395	2	28	theme	oligosaccharide	290:304	arg1	mixture					306:312	a heterogeneous oligosaccharide mixture	274:312	a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts	274:356	Such isolates contain a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts.
27559395	3	29	theme	defined	369:375	arg1	structures					405:414	defined synthetic S. pneumoniae CPS structures	369:414	defined synthetic S. pneumoniae CPS structures	369:414	Access to defined synthetic S. pneumoniae CPS structures is desirable.
27559395	2	30	theme	heterogeneous	276:288	arg1	mixture					306:312	a heterogeneous oligosaccharide mixture	274:312	a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts	274:356	Such isolates contain a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts.
27559395	7	31	theme	synthetic	1059:1067	arg1	cycle					1069:1073	the synthetic cycle	1055:1073	the synthetic cycle	1055:1073	The introduction of a washing step with the activator prior to each glycosylation cycle greatly increased the yields by neutralizing any residual base from deprotection steps in the synthetic cycle.
27559395	7	32	theme	glycosylation	945:957	arg1	cycle					959:963	each glycosylation cycle	940:963	each glycosylation cycle	940:963	The introduction of a washing step with the activator prior to each glycosylation cycle greatly increased the yields by neutralizing any residual base from deprotection steps in the synthetic cycle.
27559395	4	33	dep	serotype	463:470	arg1	CPS					474:476	CPS	474:476	S. pneumoniae serotype 3 CPS	449:476	Known syntheses of S. pneumoniae serotype 3 CPS rely on a time-consuming and low-yielding late-stage oxidation step, or use disaccharide building blocks which limits variability.
27559395	6	34	theme	late-stage	855:864	arg1	oxidation					866:874	a late-stage oxidation	853:874	a late-stage oxidation	853:874	This oligosaccharide was assembled using a novel glucuronic acid building block to circumvent the need for a late-stage oxidation.
27559395	1	35	theme	infections	137:146	arg1	one					101:103	one	101:103	one	101:103	Vaccines against S. pneumoniae, one of the most prevalent bacterial infections causing severe disease, rely on isolated capsular polysaccharide (CPS) that are conjugated to proteins.
27559395	1	35	theme	infections	137:146	arg1	Vaccines					69:76	Vaccines	69:76	Vaccines against S. pneumoniae	69:98	Vaccines against S. pneumoniae, one of the most prevalent bacterial infections causing severe disease, rely on isolated capsular polysaccharide (CPS) that are conjugated to proteins.
27559395	1	35	theme	infections	137:146	arg1	infections					137:146	the most prevalent bacterial infections	108:146	the most prevalent bacterial infections causing severe disease	108:169	Vaccines against S. pneumoniae, one of the most prevalent bacterial infections causing severe disease, rely on isolated capsular polysaccharide (CPS) that are conjugated to proteins.
27559395	0	36	theme	S.	31:32	arg1	serotype					45:52	a S. pneumoniae serotype 3	29:54	a S. pneumoniae serotype 3	29:54	Automated glycan assembly of a S. pneumoniae serotype 3 CPS antigen.
27559395	5	37	theme	conjugation-ready	684:700	arg1	trisaccharide					731:743	a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	682:743	a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	682:743	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	4	38	theme	Known	430:434	arg1	syntheses					436:444	Known syntheses	430:444	Known syntheses of S. pneumoniae serotype 3 CPS	430:476	Known syntheses of S. pneumoniae serotype 3 CPS rely on a time-consuming and low-yielding late-stage oxidation step, or use disaccharide building blocks which limits variability.
27559395	1	39	theme	isolated	180:187	arg1	CPS					214:216	CPS	214:216	CPS	214:216	Vaccines against S. pneumoniae, one of the most prevalent bacterial infections causing severe disease, rely on isolated capsular polysaccharide (CPS) that are conjugated to proteins.
27559395	1	39	theme	isolated	180:187	arg1	polysaccharide					198:211	isolated capsular polysaccharide	180:211	isolated capsular polysaccharide (CPS) that are conjugated to proteins	180:249	Vaccines against S. pneumoniae, one of the most prevalent bacterial infections causing severe disease, rely on isolated capsular polysaccharide (CPS) that are conjugated to proteins.
27559395	3	40	theme	CPS	401:403	arg1	structures					405:414	defined synthetic S. pneumoniae CPS structures	369:414	defined synthetic S. pneumoniae CPS structures	369:414	Access to defined synthetic S. pneumoniae CPS structures is desirable.
27559395	2	41	contain	contain	266:272	arg2	mixture					306:312	a heterogeneous oligosaccharide mixture	274:312	a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts	274:356	Such isolates contain a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts.
27559395	2	41	contain	contain	266:272	arg1	isolates					257:264	Such isolates	252:264	Such isolates	252:264	Such isolates contain a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts.
27559395	5	42	theme	S.	702:703	arg1	trisaccharide					731:743	a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	682:743	a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	682:743	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	4	43	theme	S.	449:450	arg1	serotype					463:470	S. pneumoniae serotype 3 CPS	449:476	S. pneumoniae serotype 3 CPS	449:476	Known syntheses of S. pneumoniae serotype 3 CPS rely on a time-consuming and low-yielding late-stage oxidation step, or use disaccharide building blocks which limits variability.
27559395	1	44	theme	capsular	189:196	arg1	CPS					214:216	CPS	214:216	CPS	214:216	Vaccines against S. pneumoniae, one of the most prevalent bacterial infections causing severe disease, rely on isolated capsular polysaccharide (CPS) that are conjugated to proteins.
27559395	1	44	theme	capsular	189:196	arg1	polysaccharide					198:211	isolated capsular polysaccharide	180:211	isolated capsular polysaccharide (CPS) that are conjugated to proteins	180:249	Vaccines against S. pneumoniae, one of the most prevalent bacterial infections causing severe disease, rely on isolated capsular polysaccharide (CPS) that are conjugated to proteins.
27559395	0	45	theme	serotype	45:52	arg1	assembly					17:24	Automated glycan assembly	0:24	Automated glycan assembly of a S. pneumoniae serotype 3	0:54	Automated glycan assembly of a S. pneumoniae serotype 3 CPS antigen.
27559395	4	46	theme	low-yielding	507:518	arg1	step					541:544	a time-consuming and low-yielding late-stage oxidation step	486:544	a time-consuming and low-yielding late-stage oxidation step	486:544	Known syntheses of S. pneumoniae serotype 3 CPS rely on a time-consuming and low-yielding late-stage oxidation step, or use disaccharide building blocks which limits variability.
27559395	7	47	theme	deprotection	1033:1044	arg1	steps					1046:1050	deprotection steps	1033:1050	deprotection steps in the synthetic cycle	1033:1073	The introduction of a washing step with the activator prior to each glycosylation cycle greatly increased the yields by neutralizing any residual base from deprotection steps in the synthetic cycle.
27559395	5	48	dep	S.	702:703	arg1	pneumoniae					705:714	pneumoniae	705:714	pneumoniae	705:714	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	7	49	theme	residual	1014:1021	arg1	base					1023:1026	any residual base	1010:1026	any residual base	1010:1026	The introduction of a washing step with the activator prior to each glycosylation cycle greatly increased the yields by neutralizing any residual base from deprotection steps in the synthetic cycle.
27559395	2	50	theme	chain	327:331	arg1	lengths					333:339	different chain lengths	317:339	different chain lengths	317:339	Such isolates contain a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts.
27559395	0	51	theme	pneumoniae	34:43	arg1	serotype					45:52	a S. pneumoniae serotype 3	29:54	a S. pneumoniae serotype 3	29:54	Automated glycan assembly of a S. pneumoniae serotype 3 CPS antigen.
27559395	4	52	theme	oxidation	531:539	arg1	step					541:544	a time-consuming and low-yielding late-stage oxidation step	486:544	a time-consuming and low-yielding late-stage oxidation step	486:544	Known syntheses of S. pneumoniae serotype 3 CPS rely on a time-consuming and low-yielding late-stage oxidation step, or use disaccharide building blocks which limits variability.
27559395	2	53	theme	different	317:325	arg1	lengths					333:339	different chain lengths	317:339	different chain lengths	317:339	Such isolates contain a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts.
27559395	7	54	theme	washing	899:905	arg1	step					907:910	a washing step	897:910	a washing step with the activator prior to each glycosylation cycle	897:963	The introduction of a washing step with the activator prior to each glycosylation cycle greatly increased the yields by neutralizing any residual base from deprotection steps in the synthetic cycle.
27559395	4	55	theme	late-stage	520:529	arg1	step					541:544	a time-consuming and low-yielding late-stage oxidation step	486:544	a time-consuming and low-yielding late-stage oxidation step	486:544	Known syntheses of S. pneumoniae serotype 3 CPS rely on a time-consuming and low-yielding late-stage oxidation step, or use disaccharide building blocks which limits variability.
27559395	7	56	theme	step	907:910	arg1	introduction					881:892	The introduction	877:892	The introduction of a washing step with the activator prior to each glycosylation cycle	877:963	The introduction of a washing step with the activator prior to each glycosylation cycle greatly increased the yields by neutralizing any residual base from deprotection steps in the synthetic cycle.
27559395	1	57	theme	prevalent	117:125	arg1	infections					137:146	the most prevalent bacterial infections	108:146	the most prevalent bacterial infections causing severe disease	108:169	Vaccines against S. pneumoniae, one of the most prevalent bacterial infections causing severe disease, rely on isolated capsular polysaccharide (CPS) that are conjugated to proteins.
27559395	5	58	theme	trisaccharide	731:743	arg1	AGA					674:676	AGA	674:676	AGA	674:676	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	5	58	theme	trisaccharide	731:743	arg1	assembly					664:671	the first iterative automated glycan assembly	627:671	the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide	627:743	Herein, we report the first iterative automated glycan assembly (AGA) of a conjugation-ready S. pneumoniae serotype 3 CPS trisaccharide.
27559395	2	59	theme	shifts	351:356	arg1	mixture					306:312	a heterogeneous oligosaccharide mixture	274:312	a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts	274:356	Such isolates contain a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts.
27559395	3	60	theme	synthetic	377:385	arg1	structures					405:414	defined synthetic S. pneumoniae CPS structures	369:414	defined synthetic S. pneumoniae CPS structures	369:414	Access to defined synthetic S. pneumoniae CPS structures is desirable.
27559395	2	61	theme	frame	345:349	arg1	shifts					351:356	frame shifts	345:356	frame shifts	345:356	Such isolates contain a heterogeneous oligosaccharide mixture of different chain lengths and frame shifts.
27559395	8	62	theme	process	1081:1087	arg1	improvement					1089:1099	This process improvement	1076:1099	This process improvement	1076:1099	This process improvement is applicable to AGA of many other oligosaccharides.
27559395	7	63	from	steps	1046:1050	arg1	cycle					1069:1073	the synthetic cycle	1055:1073	the synthetic cycle	1055:1073	The introduction of a washing step with the activator prior to each glycosylation cycle greatly increased the yields by neutralizing any residual base from deprotection steps in the synthetic cycle.
28435251	2	0	theme	WH	364:365	arg1	microspheres					374:385	WH hollow microspheres	364:385	WH hollow microspheres	364:385	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	1	1	used	utilized	224:231	arg2	Hydroxyapatite					131:144	Hydroxyapatite	131:144	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2)	131:167	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	1	1	used	utilized	224:231	arg2	whitlockite					173:183	whitlockite	173:183	whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12)	173:211	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	2	2	theme	microwave-assisted	489:506	arg1	method					521:526	microwave-assisted hydrothermal method	489:526	microwave-assisted hydrothermal method	489:526	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	3	3	from	effects	733:739	arg1	regeneration					749:760	bone regeneration	744:760	bone regeneration	744:760	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	3	3	from	effects	733:739	arg1	defects					775:781	calvarial defects	765:781	calvarial defects in a rat model	765:796	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	4	4	theme	proliferation	915:927	arg1	ability					960:966	proliferation and osteogenic differentiation ability	915:966	proliferation and osteogenic differentiation ability of human mesenchymal stem cells	915:998	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	5	5	theme	porous	1033:1038	arg1	scaffold					1052:1059	the porous WH/chitosan scaffold	1029:1059	the porous WH/chitosan scaffold	1029:1059	Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.
28435251	3	6	theme	rat	788:790	arg1	model					792:796	a rat model	786:796	a rat model	786:796	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	2	7	theme	phosphate	405:413	arg1	source					454:459	an organic phosphorus source	432:459	an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method	432:526	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	2	7	theme	phosphate	405:413	arg1	salt					424:427	creatine phosphate disodium salt	396:427	creatine phosphate disodium salt	396:427	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	5	8	theme	calvarial	1108:1116	arg1	defects					1118:1124	calvarial defects	1108:1124	calvarial defects	1108:1124	Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.
28435251	5	9	theme	porous	1233:1238	arg1	scaffold					1253:1260	porous HAP/chitosan scaffold	1233:1260	porous HAP/chitosan scaffold	1233:1260	Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.
28435251	0	10	theme	calvarial	112:120	arg1	defects					122:128	calvarial defects	112:128	calvarial defects	112:128	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects.
28435251	2	11	theme	aqueous	464:470	arg1	solution					472:479	aqueous solution	464:479	aqueous solution	464:479	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	4	12	theme	osteogenic	933:942	arg1	differentiation					944:958	osteogenic differentiation	933:958	osteogenic differentiation	933:958	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	2	13	from	source	454:459	arg1	solution					472:479	aqueous solution	464:479	aqueous solution	464:479	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	4	14	theme	human	971:975	arg1	cells					994:998	human mesenchymal stem cells	971:998	human mesenchymal stem cells	971:998	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	5	15	theme	tissue	1177:1182	arg1	engineering					1184:1194	tissue engineering	1177:1194	tissue engineering	1177:1194	Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.
28435251	3	16	theme	bone	744:747	arg1	regeneration					749:760	bone regeneration	744:760	bone regeneration	744:760	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	1	17	theme	main	269:272	arg1	they					256:259	they	256:259	they	256:259	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	1	17	theme	main	269:272	arg1	components					274:283	the main components	265:283	the main components of hard tissues such as bones and teeth	265:323	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	5	18	theme	bone	1087:1090	arg1	regeneration					1092:1103	bone regeneration	1087:1103	bone regeneration	1087:1103	Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.
28435251	1	19	dep	HAP	147:149	arg1	Ca10					152:155	Ca10(PO4)6	152:161	HAP; Ca10(PO4)6(OH)2	147:166	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	1	19	dep	HAP	147:149	arg1	OH					163:164	OH	163:164	OH	163:164	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	4	20	theme	mesenchymal	977:987	arg1	cells					994:998	human mesenchymal stem cells	971:998	human mesenchymal stem cells	971:998	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	0	21	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan	0:75	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects.
28435251	2	22	theme	hydrothermal	508:519	arg1	method					521:526	microwave-assisted hydrothermal method	489:526	microwave-assisted hydrothermal method	489:526	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	3	23	from	defects	775:781	arg1	model					792:796	a rat model	786:796	a rat model	786:796	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	1	24	theme	hard	288:291	arg1	tissues					293:299	hard tissues	288:299	hard tissues such as bones and teeth	288:323	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	1	24	theme	hard	288:291	arg1	bones					309:313	bones	309:313	bones	309:313	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	1	24	theme	hard	288:291	arg1	teeth					319:323	teeth	319:323	teeth	319:323	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	0	25	theme	porous	21:26	arg1	hydroxyapatite/chitosan					28:50	porous hydroxyapatite/chitosan	21:50	porous hydroxyapatite/chitosan	21:50	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects.
28435251	3	26	theme	WH/chitosan	677:687	arg1	scaffolds					689:697	porous HAP/chitosan and WH/chitosan scaffolds	653:697	porous HAP/chitosan and WH/chitosan scaffolds	653:697	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	4	27	theme	WH/chitosan	843:853	arg1	membrane					865:872	the WH/chitosan composite membrane	839:872	the WH/chitosan composite membrane	839:872	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	4	28	theme	stem	989:992	arg1	cells					994:998	human mesenchymal stem cells	971:998	human mesenchymal stem cells	971:998	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	3	29	theme	porous	653:658	arg1	scaffolds					689:697	porous HAP/chitosan and WH/chitosan scaffolds	653:697	porous HAP/chitosan and WH/chitosan scaffolds	653:697	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	4	30	theme	composite	855:863	arg1	membrane					865:872	the WH/chitosan composite membrane	839:872	the WH/chitosan composite membrane	839:872	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	4	31	theme	differentiation	944:958	arg1	ability					960:966	proliferation and osteogenic differentiation ability	915:966	proliferation and osteogenic differentiation ability of human mesenchymal stem cells	915:998	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	3	32	theme	HAP/chitosan	660:671	arg1	scaffolds					689:697	porous HAP/chitosan and WH/chitosan scaffolds	653:697	porous HAP/chitosan and WH/chitosan scaffolds	653:697	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	0	33	from	regeneration	96:107	arg1	defects					122:128	calvarial defects	112:128	calvarial defects	112:128	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects.
28435251	0	34	theme	hydroxyapatite/chitosan	28:50	arg1	study					12:16	Comparative study	0:16	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan	0:75	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects.
28435251	3	35	theme	freeze	702:707	arg1	drying					709:714	freeze drying	702:714	freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model	702:796	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	2	36	theme	disodium	415:422	arg1	source					454:459	an organic phosphorus source	432:459	an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method	432:526	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	2	36	theme	disodium	415:422	arg1	salt					424:427	creatine phosphate disodium salt	396:427	creatine phosphate disodium salt	396:427	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	1	37	theme	tissues	293:299	arg1	they					256:259	they	256:259	they	256:259	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	1	37	theme	tissues	293:299	arg1	components					274:283	the main components	265:283	the main components of hard tissues such as bones and teeth	265:323	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	4	38	theme	cells	994:998	arg1	ability					960:966	proliferation and osteogenic differentiation ability	915:966	proliferation and osteogenic differentiation ability of human mesenchymal stem cells	915:998	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	4	39	theme	better	880:885	arg1	biocompatibility					887:902	a better biocompatibility	878:902	a better biocompatibility	878:902	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	5	40	theme	WH/chitosan	1040:1050	arg1	scaffold					1052:1059	the porous WH/chitosan scaffold	1029:1059	the porous WH/chitosan scaffold	1029:1059	Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.
28435251	5	41	theme	calvarial	1204:1212	arg1	repair					1214:1219	calvarial repair	1204:1219	calvarial repair compared to porous HAP/chitosan scaffold	1204:1260	Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.
28435251	0	42	theme	whitlockite/chitosan	56:75	arg1	study					12:16	Comparative study	0:16	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan	0:75	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects.
28435251	4	43	theme	experimental	803:814	arg1	results					816:822	The experimental results	799:822	The experimental results	799:822	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	3	44	theme	composite	576:584	arg1	membranes					586:594	composite membranes	576:594	composite membranes	576:594	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	4	45	contain	had	874:876	arg2	biocompatibility					887:902	a better biocompatibility	878:902	a better biocompatibility	878:902	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	4	45	contain	had	874:876	arg1	membrane					865:872	the WH/chitosan composite membrane	839:872	the WH/chitosan composite membrane	839:872	The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan.
28435251	1	46	dep	WH	186:187	arg1	HPO4					198:201	Ca18Mg2(HPO4)2(PO4)12	190:210	WH; Ca18Mg2(HPO4)2(PO4)12	186:210	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	5	47	theme	HAP/chitosan	1240:1251	arg1	scaffold					1253:1260	porous HAP/chitosan scaffold	1233:1260	porous HAP/chitosan scaffold	1233:1260	Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.
28435251	2	48	theme	phosphorus	443:452	arg1	source					454:459	an organic phosphorus source	432:459	an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method	432:526	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	2	48	theme	phosphorus	443:452	arg1	salt					424:427	creatine phosphate disodium salt	396:427	creatine phosphate disodium salt	396:427	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	1	49	dep	whitlockite	173:183	arg1	WH					186:187	WH	186:187	WH; Ca18Mg2(HPO4)2(PO4)12	186:210	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	5	50	from	applications	1161:1172	arg1	engineering					1184:1194	tissue engineering	1177:1194	tissue engineering	1177:1194	Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.
28435251	2	51	theme	organic	435:441	arg1	source					454:459	an organic phosphorus source	432:459	an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method	432:526	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	2	51	theme	organic	435:441	arg1	salt					424:427	creatine phosphate disodium salt	396:427	creatine phosphate disodium salt	396:427	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	3	52	theme	calvarial	765:773	arg1	defects					775:781	calvarial defects	765:781	calvarial defects in a rat model	765:796	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	0	53	theme	bone	91:94	arg1	regeneration					96:107	bone regeneration	91:107	bone regeneration in calvarial defects	91:128	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects.
28435251	1	54	theme	Ca18Mg2	190:196	arg1	HPO4					198:201	Ca18Mg2(HPO4)2(PO4)12	190:210	WH; Ca18Mg2(HPO4)2(PO4)12	186:210	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	2	55	theme	hollow	367:372	arg1	microspheres					374:385	WH hollow microspheres	364:385	WH hollow microspheres	364:385	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	1	56	theme	bone	236:239	arg1	repair					241:246	bone repair	236:246	bone repair	236:246	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	3	57	dep	HAP/chitosan	547:558	arg1	membranes					586:594	composite membranes	576:594	composite membranes	576:594	Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model.
28435251	1	58	dep	Hydroxyapatite	131:144	arg1	HAP					147:149	HAP	147:149	HAP; Ca10(PO4)6(OH)2	147:166	Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth.
28435251	2	59	theme	creatine	396:403	arg1	source					454:459	an organic phosphorus source	432:459	an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method	432:526	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
28435251	2	59	theme	creatine	396:403	arg1	salt					424:427	creatine phosphate disodium salt	396:427	creatine phosphate disodium salt	396:427	In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method.
26394238	0	0	theme	β-glucosidase	63:75	arg1	production					77:86	β-glucosidase production	63:86	β-glucosidase production by Bacillus subtilis	63:107	A new alternative use for coffee pulp from semi-dry process to β-glucosidase production by Bacillus subtilis.
26394238	5	1	theme	rotatable	693:701	arg1	CCRD					711:714	CCRD	711:714	CCRD	711:714	The response surface methodology (RSM) based on a central composite rotatable design (CCRD) was employed for this optimization.
26394238	5	1	theme	rotatable	693:701	arg1	design					703:708	a central composite rotatable design	673:708	a central composite rotatable design (CCRD)	673:715	The response surface methodology (RSM) based on a central composite rotatable design (CCRD) was employed for this optimization.
26394238	7	2	theme	UI	959:960	arg1	ml					962:963	22·59 UI ml	953:963	22·59 UI ml(-1)	953:967	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	7	2	theme	UI	959:960	arg1	-1					965:966	-1	965:966	-1	965:966	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	2	3	theme	pulp	319:322	arg1	form					311:314	the form	307:314	the form of pulp, mucilage, husks, and water waste	307:356	During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste.
26394238	7	4	theme	36·6°C	1066:1071	arg1	temperature					1051:1061	temperature	1051:1061	temperature of 36·6°C	1051:1071	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	7	4	theme	36·6°C	1066:1071	arg1	concentration					1019:1031	coffee pulp concentration	1007:1031	coffee pulp concentration of 36·8 g l(-1)	1007:1047	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	7	4	theme	36·6°C	1066:1071	arg1	pH					1078:1079	pH	1078:1079	pH of 3·64	1078:1087	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	6	5	theme	best	831:834	arg1	conditions					836:845	the best conditions	827:845	the best conditions obtained	827:854	The methodology used in the optimization process was validated by testing the best conditions obtained and comparing them with the values predicted by the model.
26394238	7	6	theme	coffee	1007:1012	arg1	concentration					1019:1031	coffee pulp concentration	1007:1031	coffee pulp concentration of 36·8 g l(-1)	1007:1047	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	1	7	theme	preferred	146:154	arg1	drinks					169:174	the most preferred nonalcoholic drinks	137:174	the most preferred nonalcoholic drinks	137:174	UNLABELLED Coffee is among the most preferred nonalcoholic drinks, and its consumption is distributed globally.
26394238	4	8	theme	pulp	515:518	arg1	coffee					520:525	pulp coffee	515:525	pulp coffee	515:525	The aim was testify pulp coffee can be considered as carbon and inductor source for β-glucosidase by Bacillus subtilis CCMA 0087.
26394238	4	8	theme	pulp	515:518	arg1	source					568:573	carbon and inductor source	548:573	source	568:573	The aim was testify pulp coffee can be considered as carbon and inductor source for β-glucosidase by Bacillus subtilis CCMA 0087.
26394238	3	9	theme	product	486:492	arg1	growth					426:431	the growth	422:431	the growth of micro-organisms	422:450	The pulp and mucilage have the chemical composition to support the growth of micro-organisms and the production of value-added product.
26394238	3	9	theme	product	486:492	arg1	production					460:469	the production	456:469	the production of value-added product	456:492	The pulp and mucilage have the chemical composition to support the growth of micro-organisms and the production of value-added product.
26394238	8	10	theme	liquid	1212:1217	arg1	waste					1229:1233	liquid and solid waste	1212:1233	liquid and solid waste	1212:1233	SIGNIFICANCE AND IMPACT OF THE STUDY Countries whose economy is based on agricultural activities generate a great deal of liquid and solid waste.
26394238	11	11	dep	Bacillus	1528:1535	arg1	subtilis					1537:1544	subtilis	1537:1544	subtilis	1537:1544	In this study, we proposed to produce β-glucosidase production from pulp coffee extract using a Bacillus subtilis strain.
26394238	11	12	theme	β-glucosidase	1470:1482	arg1	production					1484:1493	β-glucosidase production	1470:1493	β-glucosidase production from pulp coffee extract using a Bacillus subtilis strain	1470:1551	In this study, we proposed to produce β-glucosidase production from pulp coffee extract using a Bacillus subtilis strain.
26394238	7	13	theme	culturing	994:1002	arg1	h					989:989	24 h	986:989	24 h of culturing	986:1002	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	3	14	theme	micro-organisms	436:450	arg1	growth					426:431	the growth	422:431	the growth of micro-organisms	422:450	The pulp and mucilage have the chemical composition to support the growth of micro-organisms and the production of value-added product.
26394238	3	14	theme	micro-organisms	436:450	arg1	production					460:469	the production	456:469	the production of value-added product	456:492	The pulp and mucilage have the chemical composition to support the growth of micro-organisms and the production of value-added product.
26394238	10	15	theme	such	1414:1417	arg1	production					1356:1365	The production	1352:1365	The production of enzymes	1352:1376	The production of enzymes, and particularly cellulase, is one such alternative.
26394238	10	15	theme	such	1414:1417	arg1	alternative					1419:1429	one such alternative	1410:1429	one such alternative	1410:1429	The production of enzymes, and particularly cellulase, is one such alternative.
26394238	8	16	theme	great	1198:1202	arg1	deal					1204:1207	a great deal	1196:1207	a great deal of liquid and solid waste	1196:1233	SIGNIFICANCE AND IMPACT OF THE STUDY Countries whose economy is based on agricultural activities generate a great deal of liquid and solid waste.
26394238	4	17	theme	CCMA	614:617	arg1	0087					619:622	Bacillus subtilis CCMA 0087	596:622	Bacillus subtilis CCMA 0087	596:622	The aim was testify pulp coffee can be considered as carbon and inductor source for β-glucosidase by Bacillus subtilis CCMA 0087.
26394238	1	18	theme	nonalcoholic	156:167	arg1	drinks					169:174	the most preferred nonalcoholic drinks	137:174	the most preferred nonalcoholic drinks	137:174	UNLABELLED Coffee is among the most preferred nonalcoholic drinks, and its consumption is distributed globally.
26394238	11	19	from	extract	1512:1518	arg1	production					1484:1493	β-glucosidase production	1470:1493	β-glucosidase production from pulp coffee extract using a Bacillus subtilis strain	1470:1551	In this study, we proposed to produce β-glucosidase production from pulp coffee extract using a Bacillus subtilis strain.
26394238	3	20	theme	value-added	474:484	arg1	product					486:492	value-added product	474:492	value-added product	474:492	The pulp and mucilage have the chemical composition to support the growth of micro-organisms and the production of value-added product.
26394238	7	21	dep	production	941:950	arg1	ml					962:963	22·59 UI ml	953:963	22·59 UI ml(-1)	953:967	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	7	21	dep	production	941:950	arg1	-1					965:966	-1	965:966	-1	965:966	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	0	22	theme	new	2:4	arg1	use					18:20	A new alternative use	0:20	A new alternative use for coffee pulp from semi-dry process to β-glucosidase production by Bacillus subtilis.	0:108	A new alternative use for coffee pulp from semi-dry process to β-glucosidase production by Bacillus subtilis.
26394238	10	23	theme	enzymes	1370:1376	arg1	production					1356:1365	The production	1352:1365	The production of enzymes	1352:1376	The production of enzymes, and particularly cellulase, is one such alternative.
26394238	10	23	theme	enzymes	1370:1376	arg1	alternative					1419:1429	one such alternative	1410:1429	one such alternative	1410:1429	The production of enzymes, and particularly cellulase, is one such alternative.
26394238	10	23	theme	enzymes	1370:1376	arg1	cellulase					1396:1404	cellulase	1396:1404	cellulase	1396:1404	The production of enzymes, and particularly cellulase, is one such alternative.
26394238	2	24	theme	husks	335:339	arg1	form					311:314	the form	307:314	the form of pulp, mucilage, husks, and water waste	307:356	During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste.
26394238	2	25	theme	waste	285:289	arg1	waste					285:289	waste	285:289	waste	285:289	During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste.
26394238	2	25	theme	waste	285:289	arg1	amount					275:280	a large amount	267:280	a large amount of waste	267:289	During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste.
26394238	7	26	theme	g	1041:1041	arg1	l					1043:1043	36·8 g l	1036:1043	36·8 g l(-1)	1036:1047	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	7	26	theme	g	1041:1041	arg1	-1					1045:1046	-1	1045:1046	-1	1045:1046	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	4	27	dep	Bacillus	596:603	arg1	subtilis					605:612	subtilis	605:612	subtilis	605:612	The aim was testify pulp coffee can be considered as carbon and inductor source for β-glucosidase by Bacillus subtilis CCMA 0087.
26394238	5	28	theme	surface	638:644	arg1	methodology					646:656	The response surface methodology	625:656	The response surface methodology (RSM) based on a central composite rotatable design (CCRD)	625:715	The response surface methodology (RSM) based on a central composite rotatable design (CCRD) was employed for this optimization.
26394238	5	28	theme	surface	638:644	arg1	RSM					659:661	RSM	659:661	RSM	659:661	The response surface methodology (RSM) based on a central composite rotatable design (CCRD) was employed for this optimization.
26394238	0	29	theme	alternative	6:16	arg1	use					18:20	A new alternative use	0:20	A new alternative use for coffee pulp from semi-dry process to β-glucosidase production by Bacillus subtilis.	0:108	A new alternative use for coffee pulp from semi-dry process to β-glucosidase production by Bacillus subtilis.
26394238	11	30	theme	coffee	1505:1510	arg1	extract					1512:1518	pulp coffee extract	1500:1518	pulp coffee extract using a Bacillus subtilis strain	1500:1551	In this study, we proposed to produce β-glucosidase production from pulp coffee extract using a Bacillus subtilis strain.
26394238	7	31	theme	3·64	1084:1087	arg1	temperature					1051:1061	temperature	1051:1061	temperature of 36·6°C	1051:1071	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	7	31	theme	3·64	1084:1087	arg1	concentration					1019:1031	coffee pulp concentration	1007:1031	coffee pulp concentration of 36·8 g l(-1)	1007:1047	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	7	31	theme	3·64	1084:1087	arg1	pH					1078:1079	pH	1078:1079	pH of 3·64	1078:1087	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	4	32	theme	inductor	559:566	arg1	source					568:573	carbon and inductor source	548:573	source	568:573	The aim was testify pulp coffee can be considered as carbon and inductor source for β-glucosidase by Bacillus subtilis CCMA 0087.
26394238	4	32	theme	inductor	559:566	arg1	coffee					520:525	pulp coffee	515:525	pulp coffee	515:525	The aim was testify pulp coffee can be considered as carbon and inductor source for β-glucosidase by Bacillus subtilis CCMA 0087.
26394238	0	33	theme	coffee	26:31	arg1	pulp					33:36	coffee pulp	26:36	coffee pulp from semi-dry process to β-glucosidase production by Bacillus subtilis	26:107	A new alternative use for coffee pulp from semi-dry process to β-glucosidase production by Bacillus subtilis.
26394238	8	34	theme	STUDY	1121:1125	arg1	Countries					1127:1135	THE STUDY Countries	1117:1135	THE STUDY Countries whose economy is based on agricultural activities	1117:1185	SIGNIFICANCE AND IMPACT OF THE STUDY Countries whose economy is based on agricultural activities generate a great deal of liquid and solid waste.
26394238	6	35	theme	optimization	781:792	arg1	process					794:800	the optimization process	777:800	the optimization process	777:800	The methodology used in the optimization process was validated by testing the best conditions obtained and comparing them with the values predicted by the model.
26394238	8	36	theme	waste	1229:1233	arg1	deal					1204:1207	a great deal	1196:1207	a great deal of liquid and solid waste	1196:1233	SIGNIFICANCE AND IMPACT OF THE STUDY Countries whose economy is based on agricultural activities generate a great deal of liquid and solid waste.
26394238	2	37	theme	waste	352:356	arg1	form					311:314	the form	307:314	the form of pulp, mucilage, husks, and water waste	307:356	During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste.
26394238	4	38	theme	Bacillus	596:603	arg1	0087					619:622	Bacillus subtilis CCMA 0087	596:622	Bacillus subtilis CCMA 0087	596:622	The aim was testify pulp coffee can be considered as carbon and inductor source for β-glucosidase by Bacillus subtilis CCMA 0087.
26394238	0	39	from	process	52:58	arg1	pulp					33:36	coffee pulp	26:36	coffee pulp from semi-dry process to β-glucosidase production by Bacillus subtilis	26:107	A new alternative use for coffee pulp from semi-dry process to β-glucosidase production by Bacillus subtilis.
26394238	8	40	theme	solid	1223:1227	arg1	waste					1229:1233	liquid and solid waste	1212:1233	liquid and solid waste	1212:1233	SIGNIFICANCE AND IMPACT OF THE STUDY Countries whose economy is based on agricultural activities generate a great deal of liquid and solid waste.
26394238	3	41	theme	chemical	390:397	arg1	composition					399:409	the chemical composition	386:409	the chemical composition to support the growth of micro-organisms and the production of value-added product	386:492	The pulp and mucilage have the chemical composition to support the growth of micro-organisms and the production of value-added product.
26394238	7	42	theme	pulp	1014:1017	arg1	concentration					1019:1031	coffee pulp concentration	1007:1031	coffee pulp concentration of 36·8 g l(-1)	1007:1047	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	5	43	theme	composite	683:691	arg1	CCRD					711:714	CCRD	711:714	CCRD	711:714	The response surface methodology (RSM) based on a central composite rotatable design (CCRD) was employed for this optimization.
26394238	5	43	theme	composite	683:691	arg1	design					703:708	a central composite rotatable design	673:708	a central composite rotatable design (CCRD)	673:715	The response surface methodology (RSM) based on a central composite rotatable design (CCRD) was employed for this optimization.
26394238	8	44	theme	Countries	1127:1135	arg1	IMPACT					1107:1112	IMPACT	1107:1112	IMPACT OF THE STUDY Countries whose economy is based on agricultural activities	1107:1185	SIGNIFICANCE AND IMPACT OF THE STUDY Countries whose economy is based on agricultural activities generate a great deal of liquid and solid waste.
26394238	8	44	theme	Countries	1127:1135	arg1	SIGNIFICANCE					1090:1101	SIGNIFICANCE	1090:1101	SIGNIFICANCE	1090:1101	SIGNIFICANCE AND IMPACT OF THE STUDY Countries whose economy is based on agricultural activities generate a great deal of liquid and solid waste.
26394238	11	45	theme	Bacillus	1528:1535	arg1	strain					1546:1551	a Bacillus subtilis strain	1526:1551	a Bacillus subtilis strain	1526:1551	In this study, we proposed to produce β-glucosidase production from pulp coffee extract using a Bacillus subtilis strain.
26394238	7	46	theme	highest	919:925	arg1	production					941:950	The highest β-glucosidase production	915:950	The highest β-glucosidase production (22·59 UI ml(-1) )	915:969	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	1	47	theme	UNLABELLED	110:119	arg1	Coffee					121:126	UNLABELLED Coffee	110:126	UNLABELLED Coffee	110:126	UNLABELLED Coffee is among the most preferred nonalcoholic drinks, and its consumption is distributed globally.
26394238	7	48	theme	β-glucosidase	927:939	arg1	production					941:950	The highest β-glucosidase production	915:950	The highest β-glucosidase production (22·59 UI ml(-1) )	915:969	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	5	49	theme	response	629:636	arg1	methodology					646:656	The response surface methodology	625:656	The response surface methodology (RSM) based on a central composite rotatable design (CCRD)	625:715	The response surface methodology (RSM) based on a central composite rotatable design (CCRD) was employed for this optimization.
26394238	5	49	theme	response	629:636	arg1	RSM					659:661	RSM	659:661	RSM	659:661	The response surface methodology (RSM) based on a central composite rotatable design (CCRD) was employed for this optimization.
26394238	3	50	contain	have	381:384	arg1	mucilage					372:379	mucilage	372:379	mucilage	372:379	The pulp and mucilage have the chemical composition to support the growth of micro-organisms and the production of value-added product.
26394238	3	50	contain	have	381:384	arg1	pulp					363:366	pulp	363:366	pulp	363:366	The pulp and mucilage have the chemical composition to support the growth of micro-organisms and the production of value-added product.
26394238	3	50	contain	have	381:384	arg2	composition					399:409	the chemical composition	386:409	the chemical composition to support the growth of micro-organisms and the production of value-added product	386:492	The pulp and mucilage have the chemical composition to support the growth of micro-organisms and the production of value-added product.
26394238	2	51	theme	large	269:273	arg1	waste					285:289	waste	285:289	waste	285:289	During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste.
26394238	2	51	theme	large	269:273	arg1	amount					275:280	a large amount	267:280	a large amount of waste	267:289	During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste.
26394238	9	52	theme	new	1269:1271	arg1	alternatives					1273:1284	new alternatives	1269:1284	new alternatives for using this waste	1269:1305	Thus, it is important to develop new alternatives for using this waste rather than disposing it in the environment.
26394238	8	53	theme	agricultural	1163:1174	arg1	activities					1176:1185	agricultural activities	1163:1185	agricultural activities	1163:1185	SIGNIFICANCE AND IMPACT OF THE STUDY Countries whose economy is based on agricultural activities generate a great deal of liquid and solid waste.
26394238	5	54	theme	central	675:681	arg1	CCRD					711:714	CCRD	711:714	CCRD	711:714	The response surface methodology (RSM) based on a central composite rotatable design (CCRD) was employed for this optimization.
26394238	5	54	theme	central	675:681	arg1	design					703:708	a central composite rotatable design	673:708	a central composite rotatable design (CCRD)	673:715	The response surface methodology (RSM) based on a central composite rotatable design (CCRD) was employed for this optimization.
26394238	2	55	theme	water	346:350	arg1	waste					352:356	water waste	346:356	water waste	346:356	During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste.
26394238	2	56	theme	fruiting	240:247	arg1	process					249:255	the coffee fruiting process	229:255	the coffee fruiting process	229:255	During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste.
26394238	2	57	theme	mucilage	325:332	arg1	form					311:314	the form	307:314	the form of pulp, mucilage, husks, and water waste	307:356	During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste.
26394238	0	58	theme	semi-dry	43:50	arg1	process					52:58	semi-dry process	43:58	semi-dry process	43:58	A new alternative use for coffee pulp from semi-dry process to β-glucosidase production by Bacillus subtilis.
26394238	2	59	theme	coffee	233:238	arg1	process					249:255	the coffee fruiting process	229:255	the coffee fruiting process	229:255	During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste.
26394238	7	60	theme	l	1043:1043	arg1	temperature					1051:1061	temperature	1051:1061	temperature of 36·6°C	1051:1071	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	7	60	theme	l	1043:1043	arg1	concentration					1019:1031	coffee pulp concentration	1007:1031	coffee pulp concentration of 36·8 g l(-1)	1007:1047	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	7	60	theme	l	1043:1043	arg1	pH					1078:1079	pH	1078:1079	pH of 3·64	1078:1087	The highest β-glucosidase production (22·59 UI ml(-1) ) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l(-1) , temperature of 36·6°C, and pH of 3·64.
26394238	11	61	theme	pulp	1500:1503	arg1	extract					1512:1518	pulp coffee extract	1500:1518	pulp coffee extract using a Bacillus subtilis strain	1500:1551	In this study, we proposed to produce β-glucosidase production from pulp coffee extract using a Bacillus subtilis strain.
26394238	4	62	theme	carbon	548:553	arg1	source					568:573	carbon and inductor source	548:573	source	568:573	The aim was testify pulp coffee can be considered as carbon and inductor source for β-glucosidase by Bacillus subtilis CCMA 0087.
26394238	4	62	theme	carbon	548:553	arg1	coffee					520:525	pulp coffee	515:525	pulp coffee	515:525	The aim was testify pulp coffee can be considered as carbon and inductor source for β-glucosidase by Bacillus subtilis CCMA 0087.
29213126	8	0	theme	varying	1257:1263	arg1	microenvironments					1265:1281	these spatially varying microenvironments	1241:1281	these spatially varying microenvironments	1241:1281	Moreover, these spatially varying microenvironments were found to have a significant effect on the fate of MSCs within the alginate bioinks, with stiffer regions of the bioprinted construct preferentially supporting osteogenesis over adipogenesis.
29213126	7	1	dep	alginate	1078:1085	arg1	the					1069:1071	the	1069:1071	the	1069:1071	Using the same alginate and crosslinker, but varying the crosslinking ratio, it is possible to bioprint constructs with spatially varying mechanical microenvironments.
29213126	2	2	theme	properties	344:353	arg1	modulation					303:312	modulation	303:312	modulation of alginate bioink mechanical properties	303:353	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	8	3	theme	construct	1411:1419	arg1	regions					1385:1391	stiffer regions	1377:1391	stiffer regions of the bioprinted construct preferentially supporting osteogenesis over adipogenesis	1377:1476	Moreover, these spatially varying microenvironments were found to have a significant effect on the fate of MSCs within the alginate bioinks, with stiffer regions of the bioprinted construct preferentially supporting osteogenesis over adipogenesis.
29213126	2	4	theme	mechanical	333:342	arg1	properties					344:353	alginate bioink mechanical properties	317:353	alginate bioink mechanical properties	317:353	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	0	5	theme	Spatially	94:102	arg1	Fate					115:118	Spatially Direct MSC Fate	94:118	Spatially Direct MSC Fate within Bioprinted Tissues	94:144	Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues.
29213126	2	6	theme	mesenchymal	242:252	arg1	MSC					265:267	MSC	265:267	MSC	265:267	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	2	6	theme	mesenchymal	242:252	arg1	cell					259:262	mesenchymal stem cell	242:262	mesenchymal stem cell (MSC) differentiation	242:284	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	4	7	theme	3D	814:815	arg1	constructs					825:834	3D printed constructs	814:834	3D printed constructs	814:834	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	8	8	theme	MSCs	1338:1341	arg1	fate					1330:1333	the fate	1326:1333	the fate of MSCs within the alginate bioinks	1326:1369	Moreover, these spatially varying microenvironments were found to have a significant effect on the fate of MSCs within the alginate bioinks, with stiffer regions of the bioprinted construct preferentially supporting osteogenesis over adipogenesis.
29213126	5	9	theme	factor	891:896	arg1	release					898:904	growth factor release	884:904	growth factor release from the bioinks	884:921	These same factors are also shown to influence growth factor release from the bioinks.
29213126	5	10	theme	growth	884:889	arg1	release					898:904	growth factor release	884:904	growth factor release from the bioinks	884:921	These same factors are also shown to influence growth factor release from the bioinks.
29213126	7	11	theme	crosslinking	1120:1131	arg1	ratio					1133:1137	the crosslinking ratio	1116:1137	the crosslinking ratio	1116:1137	Using the same alginate and crosslinker, but varying the crosslinking ratio, it is possible to bioprint constructs with spatially varying mechanical microenvironments.
29213126	4	12	theme	crosslinker	712:722	arg1	bioinks					672:678	such alginate bioinks	658:678	such alginate bioinks	658:678	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	4	12	theme	crosslinker	712:722	arg1	choice					696:701	the choice	692:701	the choice of ionic crosslinker	692:722	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	7	13	with	constructs	1167:1176	arg1	microenvironments					1212:1228	spatially varying mechanical microenvironments	1183:1228	spatially varying mechanical microenvironments	1183:1228	Using the same alginate and crosslinker, but varying the crosslinking ratio, it is possible to bioprint constructs with spatially varying mechanical microenvironments.
29213126	4	14	theme	bioinks	672:678	arg1	MW					652:653	the MW	648:653	the MW of such alginate bioinks, as well as the choice of ionic crosslinker,	648:723	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	0	15	theme	MSC	111:113	arg1	Fate					115:118	Spatially Direct MSC Fate	94:118	Spatially Direct MSC Fate within Bioprinted Tissues	94:144	Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues.
29213126	3	16	theme	printability	472:483	arg1	window					485:490	a printability window	470:490	a printability window for alginate of differing molecular weight (MW) by systematically varying the ratio of alginate to ionic crosslinker within the bioink	470:625	In this study, we define a printability window for alginate of differing molecular weight (MW) by systematically varying the ratio of alginate to ionic crosslinker within the bioink.
29213126	3	17	theme	molecular	518:526	arg1	MW					536:537	MW	536:537	MW	536:537	In this study, we define a printability window for alginate of differing molecular weight (MW) by systematically varying the ratio of alginate to ionic crosslinker within the bioink.
29213126	3	17	theme	molecular	518:526	arg1	weight					528:533	molecular weight	518:533	molecular weight (MW)	518:538	In this study, we define a printability window for alginate of differing molecular weight (MW) by systematically varying the ratio of alginate to ionic crosslinker within the bioink.
29213126	0	18	theme	Direct	104:109	arg1	Fate					115:118	Spatially Direct MSC Fate	94:118	Spatially Direct MSC Fate within Bioprinted Tissues	94:144	Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues.
29213126	2	19	theme	cell	259:262	arg1	differentiation					270:284	mesenchymal stem cell (MSC) differentiation	242:284	mesenchymal stem cell (MSC) differentiation	242:284	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	5	20	from	bioinks	915:921	arg1	release					898:904	growth factor release	884:904	growth factor release from the bioinks	884:921	These same factors are also shown to influence growth factor release from the bioinks.
29213126	1	21	theme	used	170:173	arg1	bioink					175:180	a commonly used bioink	159:180	a commonly used bioink in 3D bioprinting	159:198	Alginate is a commonly used bioink in 3D bioprinting.
29213126	1	21	theme	used	170:173	arg1	Alginate					147:154	Alginate	147:154	Alginate	147:154	Alginate is a commonly used bioink in 3D bioprinting.
29213126	0	22	theme	Alginate	7:14	arg1	Bioink					16:21	Tuning Alginate Bioink Stiffness and Composition	0:47	Bioink	16:21	Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues.
29213126	4	23	theme	Degradation	793:803	arg1	Modulus					784:790	Young's Modulus	776:790	Young's Modulus	776:790	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	4	23	theme	Degradation	793:803	arg1	Rate					805:808	Degradation Rate	793:808	Degradation Rate	793:808	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	4	24	theme	choice	696:701	arg1	MW					652:653	the MW	648:653	the MW of such alginate bioinks, as well as the choice of ionic crosslinker,	648:723	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	6	25	theme	hydrogels	996:1004	arg1	stiffness					969:977	the stiffness	965:977	the stiffness of 3D bioprinted hydrogels	965:1004	We next explored if spatially modulating the stiffness of 3D bioprinted hydrogels could be used to direct MSC fate inside printed tissues.
29213126	0	26	theme	Tuning	0:5	arg1	Bioink					16:21	Tuning Alginate Bioink Stiffness and Composition	0:47	Bioink	16:21	Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues.
29213126	2	27	theme	promising	368:376	arg1	strategy					378:385	a promising strategy	366:385	a promising strategy to spatially regulate MSC fate within bioprinted tissues	366:442	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	7	28	theme	bioprint	1158:1165	arg1	constructs					1167:1176	bioprint constructs	1158:1176	bioprint constructs with spatially varying mechanical microenvironments	1158:1228	Using the same alginate and crosslinker, but varying the crosslinking ratio, it is possible to bioprint constructs with spatially varying mechanical microenvironments.
29213126	4	29	theme	ionic	706:710	arg1	crosslinker					712:722	ionic crosslinker	706:722	ionic crosslinker	706:722	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	1	30	theme	3D	185:186	arg1	bioprinting					188:198	3D bioprinting	185:198	3D bioprinting	185:198	Alginate is a commonly used bioink in 3D bioprinting.
29213126	2	31	theme	bioprinted	425:434	arg1	tissues					436:442	bioprinted tissues	425:442	bioprinted tissues	425:442	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	2	32	theme	differentiation	270:284	arg1	stiffness					208:216	Matrix stiffness	201:216	Matrix stiffness	201:216	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	2	32	theme	differentiation	270:284	arg1	determinant					227:237	a key determinant	221:237	a key determinant of mesenchymal stem cell (MSC) differentiation	221:284	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	8	33	theme	significant	1304:1314	arg1	effect					1316:1321	a significant effect	1302:1321	a significant effect	1302:1321	Moreover, these spatially varying microenvironments were found to have a significant effect on the fate of MSCs within the alginate bioinks, with stiffer regions of the bioprinted construct preferentially supporting osteogenesis over adipogenesis.
29213126	6	34	theme	MSC	1030:1032	arg1	fate					1034:1037	MSC fate	1030:1037	MSC fate	1030:1037	We next explored if spatially modulating the stiffness of 3D bioprinted hydrogels could be used to direct MSC fate inside printed tissues.
29213126	6	35	theme	printed	1046:1052	arg1	tissues					1054:1060	printed tissues	1046:1060	printed tissues	1046:1060	We next explored if spatially modulating the stiffness of 3D bioprinted hydrogels could be used to direct MSC fate inside printed tissues.
29213126	4	36	dep	properties	764:773	arg1	Modulus					784:790	Young's Modulus	776:790	Young's Modulus	776:790	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	4	36	dep	properties	764:773	arg1	Rate					805:808	Degradation Rate	793:808	Degradation Rate	793:808	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	2	37	theme	key	223:225	arg1	stiffness					208:216	Matrix stiffness	201:216	Matrix stiffness	201:216	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	2	37	theme	key	223:225	arg1	determinant					227:237	a key determinant	221:237	a key determinant of mesenchymal stem cell (MSC) differentiation	221:284	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	6	38	theme	bioprinted	985:994	arg1	hydrogels					996:1004	3D bioprinted hydrogels	982:1004	3D bioprinted hydrogels	982:1004	We next explored if spatially modulating the stiffness of 3D bioprinted hydrogels could be used to direct MSC fate inside printed tissues.
29213126	2	39	theme	MSC	409:411	arg1	fate					413:416	MSC fate	409:416	MSC fate	409:416	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	7	40	theme	same	1073:1076	arg1	alginate					1078:1085	alginate	1078:1085	alginate	1078:1085	Using the same alginate and crosslinker, but varying the crosslinking ratio, it is possible to bioprint constructs with spatially varying mechanical microenvironments.
29213126	0	41	theme	Bioprinted	127:136	arg1	Tissues					138:144	Bioprinted Tissues	127:144	Bioprinted Tissues	127:144	Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues.
29213126	3	42	theme	alginate	579:586	arg1	ratio					570:574	the ratio	566:574	the ratio of alginate to ionic crosslinker within the bioink	566:625	In this study, we define a printability window for alginate of differing molecular weight (MW) by systematically varying the ratio of alginate to ionic crosslinker within the bioink.
29213126	0	43	theme	Controlled	53:62	arg1	Factor					71:76	Controlled Growth Factor	53:76	Controlled Growth Factor Delivery	53:85	Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues.
29213126	4	44	theme	constructs	825:834	arg1	properties					764:773	the mechanical properties	749:773	the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs	749:834	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	8	45	theme	stiffer	1377:1383	arg1	regions					1385:1391	stiffer regions	1377:1391	stiffer regions of the bioprinted construct preferentially supporting osteogenesis over adipogenesis	1377:1476	Moreover, these spatially varying microenvironments were found to have a significant effect on the fate of MSCs within the alginate bioinks, with stiffer regions of the bioprinted construct preferentially supporting osteogenesis over adipogenesis.
29213126	4	46	theme	Young	776:780	arg1	Modulus					784:790	Young's Modulus	776:790	Young's Modulus	776:790	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	4	46	theme	Young	776:780	arg1	Rate					805:808	Degradation Rate	793:808	Degradation Rate	793:808	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	2	47	theme	bioink	326:331	arg1	properties					344:353	alginate bioink mechanical properties	317:353	alginate bioink mechanical properties	317:353	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	4	48	theme	printed	817:823	arg1	constructs					825:834	3D printed constructs	814:834	3D printed constructs	814:834	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	2	49	theme	Matrix	201:206	arg1	stiffness					208:216	Matrix stiffness	201:216	Matrix stiffness	201:216	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	2	49	theme	Matrix	201:206	arg1	determinant					227:237	a key determinant	221:237	a key determinant of mesenchymal stem cell (MSC) differentiation	221:284	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	7	50	theme	varying	1193:1199	arg1	microenvironments					1212:1228	spatially varying mechanical microenvironments	1183:1228	spatially varying mechanical microenvironments	1183:1228	Using the same alginate and crosslinker, but varying the crosslinking ratio, it is possible to bioprint constructs with spatially varying mechanical microenvironments.
29213126	2	51	theme	alginate	317:324	arg1	properties					344:353	alginate bioink mechanical properties	317:353	alginate bioink mechanical properties	317:353	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	8	52	theme	alginate	1354:1361	arg1	bioinks					1363:1369	the alginate bioinks	1350:1369	the alginate bioinks	1350:1369	Moreover, these spatially varying microenvironments were found to have a significant effect on the fate of MSCs within the alginate bioinks, with stiffer regions of the bioprinted construct preferentially supporting osteogenesis over adipogenesis.
29213126	2	53	theme	stem	254:257	arg1	MSC					265:267	MSC	265:267	MSC	265:267	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	2	53	theme	stem	254:257	arg1	cell					259:262	mesenchymal stem cell	242:262	mesenchymal stem cell (MSC) differentiation	242:284	Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues.
29213126	4	54	theme	mechanical	753:762	arg1	properties					764:773	the mechanical properties	749:773	the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs	749:834	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	4	55	theme	alginate	663:670	arg1	bioinks					672:678	such alginate bioinks	658:678	such alginate bioinks	658:678	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	5	56	theme	same	843:846	arg1	factors					848:854	These same factors	837:854	These same factors	837:854	These same factors are also shown to influence growth factor release from the bioinks.
29213126	0	57	dep	Bioink	16:21	arg1	Stiffness					23:31	Stiffness	23:31	Stiffness	23:31	Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues.
29213126	1	58	from	bioink	175:180	arg1	bioprinting					188:198	3D bioprinting	185:198	3D bioprinting	185:198	Alginate is a commonly used bioink in 3D bioprinting.
29213126	0	59	theme	Factor	71:76	arg1	Delivery					78:85	Controlled Growth Factor Delivery	53:85	Controlled Growth Factor Delivery	53:85	Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues.
29213126	8	60	theme	bioprinted	1400:1409	arg1	construct					1411:1419	the bioprinted construct	1396:1419	the bioprinted construct preferentially supporting osteogenesis over adipogenesis	1396:1476	Moreover, these spatially varying microenvironments were found to have a significant effect on the fate of MSCs within the alginate bioinks, with stiffer regions of the bioprinted construct preferentially supporting osteogenesis over adipogenesis.
29213126	6	61	theme	3D	982:983	arg1	hydrogels					996:1004	3D bioprinted hydrogels	982:1004	3D bioprinted hydrogels	982:1004	We next explored if spatially modulating the stiffness of 3D bioprinted hydrogels could be used to direct MSC fate inside printed tissues.
29213126	4	62	theme	such	658:661	arg1	bioinks					672:678	such alginate bioinks	658:678	such alginate bioinks	658:678	We demonstrate that the MW of such alginate bioinks, as well as the choice of ionic crosslinker, can be tuned to control the mechanical properties (Young's Modulus, Degradation Rate) of 3D printed constructs.
29213126	0	63	theme	Growth	64:69	arg1	Factor					71:76	Controlled Growth Factor	53:76	Controlled Growth Factor Delivery	53:85	Tuning Alginate Bioink Stiffness and Composition for Controlled Growth Factor Delivery and to Spatially Direct MSC Fate within Bioprinted Tissues.
29213126	3	64	theme	ionic	591:595	arg1	crosslinker					597:607	ionic crosslinker	591:607	ionic crosslinker within the bioink	591:625	In this study, we define a printability window for alginate of differing molecular weight (MW) by systematically varying the ratio of alginate to ionic crosslinker within the bioink.
29213126	7	65	theme	mechanical	1201:1210	arg1	microenvironments					1212:1228	spatially varying mechanical microenvironments	1183:1228	spatially varying mechanical microenvironments	1183:1228	Using the same alginate and crosslinker, but varying the crosslinking ratio, it is possible to bioprint constructs with spatially varying mechanical microenvironments.
29213126	8	66	contain	have	1297:1300	arg2	effect					1316:1321	a significant effect	1302:1321	a significant effect	1302:1321	Moreover, these spatially varying microenvironments were found to have a significant effect on the fate of MSCs within the alginate bioinks, with stiffer regions of the bioprinted construct preferentially supporting osteogenesis over adipogenesis.
29213126	8	66	contain	have	1297:1300	arg1	microenvironments					1265:1281	these spatially varying microenvironments	1241:1281	these spatially varying microenvironments	1241:1281	Moreover, these spatially varying microenvironments were found to have a significant effect on the fate of MSCs within the alginate bioinks, with stiffer regions of the bioprinted construct preferentially supporting osteogenesis over adipogenesis.
26572341	5	0	theme	phosphate	772:780	arg1	crystals					789:796	bioactive calcium phosphate (Ca-P) crystals	754:796	bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution	754:835	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	1	1	theme	based	151:155	arg1	scaffolds					157:165	cellulose based scaffolds	141:165	cellulose based scaffolds	141:165	In this study, cellulose based scaffolds were produced by electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h.
26572341	4	2	theme	porous	560:565	arg1	mesh					594:597	an open porous and loosely packed fibrous mesh	552:597	an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane	552:694	SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane.
26572341	7	3	theme	better	1078:1083	arg1	infiltration					1090:1101	the better cell infiltration	1074:1101	the better cell infiltration	1074:1101	3D cellulose sponge exhibited the better cell infiltration, spreading and proliferation compared to 2D cellulose mat.
26572341	8	4	theme	improved	1222:1229	arg1	mineralization					1231:1244	improved mineralization	1222:1244	improved mineralization	1222:1244	Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.
26572341	3	5	theme	three-dimensional	487:503	arg1	sponge					520:525	a three-dimensional (3D) cellulose sponge	485:525	a three-dimensional (3D) cellulose sponge	485:525	In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge.
26572341	4	6	theme	open	555:558	arg1	mesh					594:597	an open porous and loosely packed fibrous mesh	552:597	an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane	552:694	SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane.
26572341	5	7	theme	admirable	724:732	arg1	ability					734:740	admirable ability	724:740	admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution	724:835	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	6	8	theme	X-ray	850:854	arg1	studies					868:874	X-ray diffraction studies	850:874	X-ray diffraction studies	850:874	SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone.
26572341	0	9	theme	in	98:99	arg1	infiltration					112:123	in vitro cell infiltration	98:123	in vitro cell infiltration	98:123	Three-dimensional cellulose sponge: Fabrication, characterization, biomimetic mineralization, and in vitro cell infiltration.
26572341	5	10	theme	body	811:814	arg1	SBF					823:825	SBF	823:825	SBF	823:825	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	5	10	theme	body	811:814	arg1	fluid					816:820	simulated body fluid	801:820	simulated body fluid (SBF) solution	801:835	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	2	11	theme	sodium	354:359	arg1	SB					374:375	SB	374:375	SB	374:375	The resulting nonwoven cellulose mat was treated with sodium borohydride (SB) solution.
26572341	2	11	theme	sodium	354:359	arg1	borohydride					361:371	sodium borohydride	354:371	sodium borohydride (SB) solution	354:385	The resulting nonwoven cellulose mat was treated with sodium borohydride (SB) solution.
26572341	2	12	theme	cellulose	323:331	arg1	mat					333:335	The resulting nonwoven cellulose mat	300:335	The resulting nonwoven cellulose mat	300:335	The resulting nonwoven cellulose mat was treated with sodium borohydride (SB) solution.
26572341	8	13	theme	facile	1175:1180	arg1	fabrication					1182:1192	a facile fabrication	1173:1192	a facile fabrication of 3D cellulose sponge with improved mineralization	1173:1244	Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.
26572341	3	14	dep	In	388:389	arg1	situ					391:394	situ	391:394	situ	391:394	In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge.
26572341	3	15	theme	membrane	444:451	arg1	pores					431:435	the pores	427:435	the pores of the membrane	427:451	In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge.
26572341	0	16	theme	cell	107:110	arg1	infiltration					112:123	in vitro cell infiltration	98:123	in vitro cell infiltration	98:123	Three-dimensional cellulose sponge: Fabrication, characterization, biomimetic mineralization, and in vitro cell infiltration.
26572341	7	17	theme	2D	1144:1145	arg1	mat					1157:1159	2D cellulose mat	1144:1159	2D cellulose mat	1144:1159	3D cellulose sponge exhibited the better cell infiltration, spreading and proliferation compared to 2D cellulose mat.
26572341	0	18	dep	in	98:99	arg1	vitro					101:105	vitro	101:105	vitro	101:105	Three-dimensional cellulose sponge: Fabrication, characterization, biomimetic mineralization, and in vitro cell infiltration.
26572341	4	19	theme	single-layered	630:643	arg1	structure					645:653	the tightly packed single-layered structure	611:653	the tightly packed single-layered structure of the conventional electrospun membrane	611:694	SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane.
26572341	5	20	theme	simulated	801:809	arg1	SBF					823:825	SBF	823:825	SBF	823:825	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	5	20	theme	simulated	801:809	arg1	fluid					816:820	simulated body fluid	801:820	simulated body fluid (SBF) solution	801:835	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	7	21	theme	cell	1085:1088	arg1	infiltration					1090:1101	the better cell infiltration	1074:1101	the better cell infiltration	1074:1101	3D cellulose sponge exhibited the better cell infiltration, spreading and proliferation compared to 2D cellulose mat.
26572341	2	22	theme	nonwoven	314:321	arg1	mat					333:335	The resulting nonwoven cellulose mat	300:335	The resulting nonwoven cellulose mat	300:335	The resulting nonwoven cellulose mat was treated with sodium borohydride (SB) solution.
26572341	3	23	theme	SB	410:411	arg1	solution					413:420	SB solution	410:420	SB solution	410:420	In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge.
26572341	0	24	theme	Three-dimensional	0:16	arg1	cellulose					18:26	Three-dimensional cellulose	0:26	Three-dimensional cellulose	0:26	Three-dimensional cellulose sponge: Fabrication, characterization, biomimetic mineralization, and in vitro cell infiltration.
26572341	1	25	theme	NaOH/ethanol	271:282	arg1	system					284:289	NaOH/ethanol system	271:289	NaOH/ethanol system for 24h	271:297	In this study, cellulose based scaffolds were produced by electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h.
26572341	2	26	theme	resulting	304:312	arg1	mat					333:335	The resulting nonwoven cellulose mat	300:335	The resulting nonwoven cellulose mat	300:335	The resulting nonwoven cellulose mat was treated with sodium borohydride (SB) solution.
26572341	3	27	theme	solution	413:420	arg1	hydrolysis					396:405	In situ hydrolysis	388:405	In situ hydrolysis of SB solution into the pores of the membrane	388:451	In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge.
26572341	5	28	theme	fluid	816:820	arg1	solution					828:835	simulated body fluid (SBF) solution	801:835	simulated body fluid (SBF) solution	801:835	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	4	29	theme	SEM	528:530	arg1	images					532:537	SEM images	528:537	SEM images	528:537	SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane.
26572341	5	30	theme	3D	697:698	arg1	sponge					710:715	3D cellulose sponge	697:715	3D cellulose sponge	697:715	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	3	31	dep	three-dimensional	487:503	arg1	3D					506:507	3D	506:507	3D	506:507	In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge.
26572341	6	32	theme	similar	970:976	arg1	composition					958:968	the nonstoichiometric composition	936:968	the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone	936:1041	SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone.
26572341	5	33	theme	cellulose	700:708	arg1	sponge					710:715	3D cellulose sponge	697:715	3D cellulose sponge	697:715	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	6	34	theme	diffraction	856:866	arg1	studies					868:874	X-ray diffraction studies	850:874	X-ray diffraction studies	850:874	SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone.
26572341	4	35	theme	membrane	687:694	arg1	structure					645:653	the tightly packed single-layered structure	611:653	the tightly packed single-layered structure of the conventional electrospun membrane	611:694	SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane.
26572341	1	36	theme	cellulose	203:211	arg1	solution					226:233	cellulose acetate (CA) solution	203:233	cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h	203:297	In this study, cellulose based scaffolds were produced by electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h.
26572341	5	37	theme	Ca-P	783:786	arg1	crystals					789:796	bioactive calcium phosphate (Ca-P) crystals	754:796	bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution	754:835	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	4	38	theme	packed	623:628	arg1	structure					645:653	the tightly packed single-layered structure	611:653	the tightly packed single-layered structure of the conventional electrospun membrane	611:694	SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane.
26572341	8	39	theme	innovative	1260:1269	arg1	strategy					1271:1278	an innovative strategy	1257:1278	an innovative strategy for the bone tissue engineering applications	1257:1323	Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.
26572341	1	40	theme	acetate	213:219	arg1	solution					226:233	cellulose acetate (CA) solution	203:233	cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h	203:297	In this study, cellulose based scaffolds were produced by electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h.
26572341	8	41	theme	sponge	1210:1215	arg1	fabrication					1182:1192	a facile fabrication	1173:1192	a facile fabrication of 3D cellulose sponge with improved mineralization	1173:1244	Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.
26572341	6	42	theme	nonstoichiometric	940:956	arg1	composition					958:968	the nonstoichiometric composition	936:968	the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone	936:1041	SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone.
26572341	4	43	dep	porous	560:565	arg1	fibrous					586:592	fibrous	586:592	fibrous	586:592	SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane.
26572341	8	44	theme	cellulose	1200:1208	arg1	sponge					1210:1215	3D cellulose sponge	1197:1215	3D cellulose sponge	1197:1215	Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.
26572341	1	45	with	saponification	251:264	arg1	system					284:289	NaOH/ethanol system	271:289	NaOH/ethanol system for 24h	271:297	In this study, cellulose based scaffolds were produced by electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h.
26572341	6	46	theme	bone	1038:1041	arg1	component					1021:1029	the mineralized component	1005:1029	the mineralized component of the bone	1005:1041	SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone.
26572341	6	46	theme	bone	1038:1041	arg1	hydroxyapatite					989:1002	hydroxyapatite	989:1002	hydroxyapatite	989:1002	SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone.
26572341	3	47	theme	In	388:389	arg1	hydrolysis					396:405	In situ hydrolysis	388:405	In situ hydrolysis of SB solution into the pores of the membrane	388:451	In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge.
26572341	7	48	theme	3D	1044:1045	arg1	sponge					1057:1062	3D cellulose sponge	1044:1062	3D cellulose sponge	1044:1062	3D cellulose sponge exhibited the better cell infiltration, spreading and proliferation compared to 2D cellulose mat.
26572341	5	49	theme	calcium	764:770	arg1	crystals					789:796	bioactive calcium phosphate (Ca-P) crystals	754:796	bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution	754:835	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	7	50	theme	cellulose	1147:1155	arg1	mat					1157:1159	2D cellulose mat	1144:1159	2D cellulose mat	1144:1159	3D cellulose sponge exhibited the better cell infiltration, spreading and proliferation compared to 2D cellulose mat.
26572341	7	51	theme	cellulose	1047:1055	arg1	sponge					1057:1062	3D cellulose sponge	1044:1062	3D cellulose sponge	1044:1062	3D cellulose sponge exhibited the better cell infiltration, spreading and proliferation compared to 2D cellulose mat.
26572341	1	52	theme	cellulose	141:149	arg1	scaffolds					157:165	cellulose based scaffolds	141:165	cellulose based scaffolds	141:165	In this study, cellulose based scaffolds were produced by electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h.
26572341	8	53	theme	engineering	1300:1310	arg1	applications					1312:1323	the bone tissue engineering applications	1284:1323	the bone tissue engineering applications	1284:1323	Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.
26572341	8	54	theme	tissue	1293:1298	arg1	applications					1312:1323	the bone tissue engineering applications	1284:1323	the bone tissue engineering applications	1284:1323	Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.
26572341	4	55	theme	conventional	662:673	arg1	membrane					687:694	the conventional electrospun membrane	658:694	the conventional electrospun membrane	658:694	SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane.
26572341	1	56	theme	CA	222:223	arg1	solution					226:233	cellulose acetate (CA) solution	203:233	cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h	203:297	In this study, cellulose based scaffolds were produced by electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h.
26572341	3	57	theme	cellulose	510:518	arg1	sponge					520:525	a three-dimensional (3D) cellulose sponge	485:525	a three-dimensional (3D) cellulose sponge	485:525	In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge.
26572341	6	58	contain	have	931:934	arg2	composition					958:968	the nonstoichiometric composition	936:968	the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone	936:1041	SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone.
26572341	6	58	contain	have	931:934	arg1	minerals					894:901	the minerals	890:901	the minerals deposited on the nanofibers	890:929	SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone.
26572341	3	59	theme	hydrogen	462:469	arg1	gas					471:473	hydrogen gas	462:473	hydrogen gas resulting a three-dimensional (3D) cellulose sponge	462:525	In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge.
26572341	4	60	theme	packed	579:584	arg1	mesh					594:597	an open porous and loosely packed fibrous mesh	552:597	an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane	552:694	SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane.
26572341	5	61	theme	bioactive	754:762	arg1	crystals					789:796	bioactive calcium phosphate (Ca-P) crystals	754:796	bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution	754:835	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	8	62	theme	bone	1288:1291	arg1	applications					1312:1323	the bone tissue engineering applications	1284:1323	the bone tissue engineering applications	1284:1323	Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.
26572341	4	63	theme	electrospun	675:685	arg1	membrane					687:694	the conventional electrospun membrane	658:694	the conventional electrospun membrane	658:694	SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane.
26572341	1	64	theme	solution	226:233	arg1	electrospinning					184:198	electrospinning	184:198	electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h	184:297	In this study, cellulose based scaffolds were produced by electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h.
26572341	0	65	theme	biomimetic	67:76	arg1	mineralization					78:91	biomimetic mineralization	67:91	biomimetic mineralization	67:91	Three-dimensional cellulose sponge: Fabrication, characterization, biomimetic mineralization, and in vitro cell infiltration.
26572341	5	66	from	crystals	789:796	arg1	solution					828:835	simulated body fluid (SBF) solution	801:835	simulated body fluid (SBF) solution	801:835	3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution.
26572341	8	67	theme	3D	1197:1198	arg1	sponge					1210:1215	3D cellulose sponge	1197:1215	3D cellulose sponge	1197:1215	Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.
26572341	2	68	theme	borohydride	361:371	arg1	solution					378:385	sodium borohydride (SB) solution	354:385	sodium borohydride (SB) solution	354:385	The resulting nonwoven cellulose mat was treated with sodium borohydride (SB) solution.
26572341	8	69	with	fabrication	1182:1192	arg1	mineralization					1231:1244	improved mineralization	1222:1244	improved mineralization	1222:1244	Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.
26572341	6	70	theme	mineralized	1009:1019	arg1	component					1021:1029	the mineralized component	1005:1029	the mineralized component of the bone	1005:1041	SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone.
26572341	6	70	theme	mineralized	1009:1019	arg1	hydroxyapatite					989:1002	hydroxyapatite	989:1002	hydroxyapatite	989:1002	SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone.
25465553	0	0	theme	red	90:92	arg1	extract					102:108	red ginseng extract	90:108	red ginseng extract	90:108	Short communication: Physicochemical and antioxidant properties of milk supplemented with red ginseng extract.
25465553	5	1	theme	total	688:692	arg1	solids					694:699	total solids	688:699	total solids	688:699	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	1	2	theme	ginseng	153:159	arg1	RGE					170:172	RGE	170:172	RGE	170:172	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	1	2	theme	ginseng	153:159	arg1	extract					161:167	red ginseng extract	149:167	red ginseng extract (RGE)	149:173	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	1	3	theme	antioxidant	228:238	arg1	activity					240:247	antioxidant activity	228:247	antioxidant activity	228:247	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	5	4	theme	solids	694:699	arg1	increase					664:671	an increase	661:671	an increase of lactose and total solids content, titratable acidity, and a* and b* values	661:749	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	9	5	with	RGE	1133:1135	arg1	oligosaccharide					1142:1156	oligosaccharide	1142:1156	oligosaccharide	1142:1156	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	9	5	with	RGE	1133:1135	arg1	cyclodextrin					1195:1206	cyclodextrin	1195:1206	cyclodextrin	1195:1206	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	9	5	with	RGE	1133:1135	arg1	oligosaccharide					1175:1189	oligosaccharide	1175:1189	oligosaccharide	1175:1189	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	1	6	theme	extract	161:167	arg1	effect					139:144	the effect	135:144	the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk	135:255	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	10	7	theme	RGE	1336:1338	arg1	flavor					1326:1331	flavor	1326:1331	flavor	1326:1331	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	10	7	theme	RGE	1336:1338	arg1	bitterness					1311:1320	bitterness	1311:1320	bitterness	1311:1320	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	10	8	theme	cyclodextrin	1245:1256	arg1	addition					1213:1220	The addition	1209:1220	The addition of oligosaccharide and cyclodextrin	1209:1256	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	7	9	theme	antioxidant	842:852	arg1	activity					854:861	The antioxidant activity	838:861	The antioxidant activity of milk samples supplemented with RGE	838:899	The antioxidant activity of milk samples supplemented with RGE was higher than that of the control sample.
25465553	7	9	theme	antioxidant	842:852	arg1	higher					905:910	higher	905:910	higher	905:910	The antioxidant activity of milk samples supplemented with RGE was higher than that of the control sample.
25465553	9	10	dep	milk	1068:1071	arg1	1					1054:1054	1	1054:1054	1	1054:1054	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	0	11	theme	ginseng	94:100	arg1	extract					102:108	red ginseng extract	90:108	red ginseng extract	90:108	Short communication: Physicochemical and antioxidant properties of milk supplemented with red ginseng extract.
25465553	9	12	theme	%	1123:1123	arg1	RGE					1125:1127	(2) 2% RGE	1118:1127	(2) 2% RGE	1118:1127	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	9	13	theme	%	1131:1131	arg1	RGE					1133:1135	2% RGE	1130:1135	2% RGE with oligosaccharide	1130:1156	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	2	14	theme	milk	262:265	arg1	samples					267:273	The milk samples	258:273	The milk samples with RGE added at 0.5, 1, 1.5, and 2%	258:311	The milk samples with RGE added at 0.5, 1, 1.5, and 2% were analyzed during storage at 4°C.
25465553	5	15	from	increase	611:618	arg1	amount					627:632	the amount	623:632	the amount of RGE in milk	623:647	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	5	15	from	increase	611:618	arg1	RGE					637:639	RGE	637:639	RGE	637:639	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	1	16	theme	milk	252:255	arg1	activity					240:247	antioxidant activity	228:247	antioxidant activity	228:247	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	1	16	theme	milk	252:255	arg1	properties					198:207	the physicochemical properties	178:207	the physicochemical properties	178:207	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	1	16	theme	milk	252:255	arg1	test					218:221	sensory test	210:221	sensory test	210:221	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	10	17	theme	flavor	1326:1331	arg1	aftertaste					1345:1354	aftertaste	1345:1354	aftertaste	1345:1354	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	10	17	theme	flavor	1326:1331	arg1	decrease					1299:1306	a decrease	1297:1306	a decrease of bitterness and flavor of RGE	1297:1338	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	10	17	theme	flavor	1326:1331	arg1	increase					1274:1281	an increase	1271:1281	an increase of sweetness	1271:1294	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	11	18	theme	functional	1416:1425	arg1	food					1427:1430	a functional food	1414:1430	a functional food	1414:1430	Therefore, milk supplemented with RGE could be useful as a functional food.
25465553	5	19	theme	b	741:741	arg1	*					742:742	b*	741:742	b*	741:742	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	4	20	theme	2,2-diphenyl-1-picrylhydrazyl	512:540	arg1	method					542:547	the 2,2-diphenyl-1-picrylhydrazyl method	508:547	the 2,2-diphenyl-1-picrylhydrazyl method	508:547	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	4	20	theme	2,2-diphenyl-1-picrylhydrazyl	512:540	arg1	assay					601:605	ferric thiocyanate assay	582:605	ferric thiocyanate assay	582:605	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	4	20	theme	2,2-diphenyl-1-picrylhydrazyl	512:540	arg1	assay					571:575	β-carotene bleaching assay	550:575	β-carotene bleaching assay	550:575	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	5	21	theme	content	701:707	arg1	values					744:749	content, titratable acidity, and a* and b* values	701:749	content, titratable acidity, and a* and b* values	701:749	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	9	22	theme	sterilized	1057:1066	arg1	milk					1068:1071	(1) sterilized milk	1053:1071	(1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin	1053:1206	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	10	23	theme	bitterness	1311:1320	arg1	aftertaste					1345:1354	aftertaste	1345:1354	aftertaste	1345:1354	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	10	23	theme	bitterness	1311:1320	arg1	decrease					1299:1306	a decrease	1297:1306	a decrease of bitterness and flavor of RGE	1297:1338	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	10	23	theme	bitterness	1311:1320	arg1	increase					1274:1281	an increase	1271:1281	an increase of sweetness	1271:1294	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	1	24	from	effect	139:144	arg1	activity					240:247	antioxidant activity	228:247	antioxidant activity	228:247	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	1	24	from	effect	139:144	arg1	properties					198:207	the physicochemical properties	178:207	the physicochemical properties	178:207	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	1	24	from	effect	139:144	arg1	test					218:221	sensory test	210:221	sensory test	210:221	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	5	25	theme	fat	760:762	arg1	contents					776:783	fat and protein contents	760:783	fat and protein contents	760:783	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	0	26	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication	0:18	Short communication: Physicochemical and antioxidant properties of milk supplemented with red ginseng extract.
25465553	4	27	theme	samples	479:485	arg1	activity					462:469	The antioxidant activity	446:469	The antioxidant activity of milk samples	446:485	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	3	28	theme	physicochemical	354:368	arg1	properties					370:379	The physicochemical properties	350:379	The physicochemical properties	350:379	The physicochemical properties included composition of milk, pH, titratable acidity, and color.
25465553	0	29	theme	Physicochemical	21:35	arg1	properties					53:62	Physicochemical and antioxidant properties	21:62	Physicochemical and antioxidant properties of milk supplemented with red ginseng extract	21:108	Short communication: Physicochemical and antioxidant properties of milk supplemented with red ginseng extract.
25465553	4	30	theme	milk	474:477	arg1	samples					479:485	milk samples	474:485	milk samples	474:485	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	5	31	theme	RGE	637:639	arg1	amount					627:632	the amount	623:632	the amount of RGE in milk	623:647	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	5	31	theme	RGE	637:639	arg1	RGE					637:639	RGE	637:639	RGE	637:639	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	9	32	used	used	1047:1050	arg2	types					1025:1029	Two types	1021:1029	Two types of samples	1021:1040	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	3	33	theme	milk	405:408	arg1	composition					390:400	composition	390:400	composition of milk, pH, titratable acidity, and color	390:443	The physicochemical properties included composition of milk, pH, titratable acidity, and color.
25465553	5	34	theme	*	735:735	arg1	values					744:749	content, titratable acidity, and a* and b* values	701:749	content, titratable acidity, and a* and b* values	701:749	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	4	35	theme	β-carotene	550:559	arg1	method					542:547	the 2,2-diphenyl-1-picrylhydrazyl method	508:547	the 2,2-diphenyl-1-picrylhydrazyl method	508:547	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	4	35	theme	β-carotene	550:559	arg1	assay					571:575	β-carotene bleaching assay	550:575	β-carotene bleaching assay	550:575	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	9	36	with	RGE	1088:1090	arg1	oligosaccharide					1142:1156	oligosaccharide	1142:1156	oligosaccharide	1142:1156	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	9	36	with	RGE	1088:1090	arg1	cyclodextrin					1195:1206	cyclodextrin	1195:1206	cyclodextrin	1195:1206	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	9	36	with	RGE	1088:1090	arg1	oligosaccharide					1175:1189	oligosaccharide	1175:1189	oligosaccharide	1175:1189	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	1	37	theme	physicochemical	182:196	arg1	properties					198:207	the physicochemical properties	178:207	the physicochemical properties	178:207	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	0	38	theme	antioxidant	41:51	arg1	properties					53:62	Physicochemical and antioxidant properties	21:62	Physicochemical and antioxidant properties of milk supplemented with red ginseng extract	21:108	Short communication: Physicochemical and antioxidant properties of milk supplemented with red ginseng extract.
25465553	5	39	dep	lactose	676:682	arg1	values					744:749	content, titratable acidity, and a* and b* values	701:749	content, titratable acidity, and a* and b* values	701:749	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	8	40	theme	Sensory	945:951	arg1	evaluation					953:962	Sensory evaluation	945:962	Sensory evaluation	945:962	Sensory evaluation was performed using a quantitative descriptive analysis.
25465553	4	41	theme	thiocyanate	589:599	arg1	method					542:547	the 2,2-diphenyl-1-picrylhydrazyl method	508:547	the 2,2-diphenyl-1-picrylhydrazyl method	508:547	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	4	41	theme	thiocyanate	589:599	arg1	assay					601:605	ferric thiocyanate assay	582:605	ferric thiocyanate assay	582:605	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	4	42	theme	antioxidant	450:460	arg1	activity					462:469	The antioxidant activity	446:469	The antioxidant activity of milk samples	446:485	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	7	43	theme	control	929:935	arg1	sample					937:942	the control sample	925:942	the control sample	925:942	The antioxidant activity of milk samples supplemented with RGE was higher than that of the control sample.
25465553	0	44	dep	properties	53:62	arg1	communication					6:18	Short communication	0:18	Short communication	0:18	Short communication: Physicochemical and antioxidant properties of milk supplemented with red ginseng extract.
25465553	9	45	theme	%	1164:1164	arg1	RGE					1166:1168	2% RGE	1163:1168	2% RGE with oligosaccharide and cyclodextrin	1163:1206	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	4	46	theme	ferric	582:587	arg1	method					542:547	the 2,2-diphenyl-1-picrylhydrazyl method	508:547	the 2,2-diphenyl-1-picrylhydrazyl method	508:547	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	4	46	theme	ferric	582:587	arg1	assay					601:605	ferric thiocyanate assay	582:605	ferric thiocyanate assay	582:605	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	5	47	theme	titratable	710:719	arg1	acidity					721:727	titratable acidity	710:727	titratable acidity	710:727	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	5	48	theme	protein	768:774	arg1	contents					776:783	fat and protein contents	760:783	fat and protein contents	760:783	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	5	49	dep	resulted	649:656	arg1	whereas					752:758	whereas	752:758	whereas	752:758	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	3	50	theme	titratable	415:424	arg1	acidity					426:432	titratable acidity	415:432	titratable acidity	415:432	The physicochemical properties included composition of milk, pH, titratable acidity, and color.
25465553	5	51	theme	acidity	721:727	arg1	values					744:749	content, titratable acidity, and a* and b* values	701:749	content, titratable acidity, and a* and b* values	701:749	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	6	52	theme	L	818:818	arg1	value					821:825	L* value	818:825	L* value	818:825	Also, pH and L* value decreased.
25465553	10	53	theme	sweetness	1286:1294	arg1	aftertaste					1345:1354	aftertaste	1345:1354	aftertaste	1345:1354	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	10	53	theme	sweetness	1286:1294	arg1	decrease					1299:1306	a decrease	1297:1306	a decrease of bitterness and flavor of RGE	1297:1338	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	10	53	theme	sweetness	1286:1294	arg1	increase					1274:1281	an increase	1271:1281	an increase of sweetness	1271:1294	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	10	54	theme	oligosaccharide	1225:1239	arg1	addition					1213:1220	The addition	1209:1220	The addition of oligosaccharide and cyclodextrin	1209:1256	The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste.
25465553	3	55	theme	acidity	426:432	arg1	composition					390:400	composition	390:400	composition of milk, pH, titratable acidity, and color	390:443	The physicochemical properties included composition of milk, pH, titratable acidity, and color.
25465553	8	56	theme	descriptive	999:1009	arg1	analysis					1011:1018	a quantitative descriptive analysis	984:1018	a quantitative descriptive analysis	984:1018	Sensory evaluation was performed using a quantitative descriptive analysis.
25465553	9	57	with	RGE	1125:1127	arg1	oligosaccharide					1142:1156	oligosaccharide	1142:1156	oligosaccharide	1142:1156	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	9	57	with	RGE	1125:1127	arg1	cyclodextrin					1195:1206	cyclodextrin	1195:1206	cyclodextrin	1195:1206	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	9	57	with	RGE	1125:1127	arg1	oligosaccharide					1175:1189	oligosaccharide	1175:1189	oligosaccharide	1175:1189	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	9	58	with	RGE	1166:1168	arg1	oligosaccharide					1142:1156	oligosaccharide	1142:1156	oligosaccharide	1142:1156	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	9	58	with	RGE	1166:1168	arg1	cyclodextrin					1195:1206	cyclodextrin	1195:1206	cyclodextrin	1195:1206	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	9	58	with	RGE	1166:1168	arg1	oligosaccharide					1175:1189	oligosaccharide	1175:1189	oligosaccharide	1175:1189	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	3	59	theme	pH	411:412	arg1	composition					390:400	composition	390:400	composition of milk, pH, titratable acidity, and color	390:443	The physicochemical properties included composition of milk, pH, titratable acidity, and color.
25465553	5	60	from	amount	627:632	arg1	milk					644:647	milk	644:647	milk	644:647	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	1	61	theme	sensory	210:216	arg1	test					218:221	sensory test	210:221	sensory test	210:221	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	8	62	theme	quantitative	986:997	arg1	analysis					1011:1018	a quantitative descriptive analysis	984:1018	a quantitative descriptive analysis	984:1018	Sensory evaluation was performed using a quantitative descriptive analysis.
25465553	9	63	theme	samples	1034:1040	arg1	types					1025:1029	Two types	1021:1029	Two types of samples	1021:1040	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	7	64	theme	milk	866:869	arg1	samples					871:877	milk samples	866:877	milk samples supplemented with RGE	866:899	The antioxidant activity of milk samples supplemented with RGE was higher than that of the control sample.
25465553	9	65	theme	2	1130:1130	arg1	%					1131:1131	%	1131:1131	%	1131:1131	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	5	66	theme	lactose	676:682	arg1	increase					664:671	an increase	661:671	an increase of lactose and total solids content, titratable acidity, and a* and b* values	661:749	An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged.
25465553	7	67	theme	samples	871:877	arg1	activity					854:861	The antioxidant activity	838:861	The antioxidant activity of milk samples supplemented with RGE	838:899	The antioxidant activity of milk samples supplemented with RGE was higher than that of the control sample.
25465553	7	67	theme	samples	871:877	arg1	higher					905:910	higher	905:910	higher	905:910	The antioxidant activity of milk samples supplemented with RGE was higher than that of the control sample.
25465553	3	68	theme	color	439:443	arg1	composition					390:400	composition	390:400	composition of milk, pH, titratable acidity, and color	390:443	The physicochemical properties included composition of milk, pH, titratable acidity, and color.
25465553	2	69	with	samples	267:273	arg1	RGE					280:282	RGE	280:282	RGE added at 0.5, 1, 1.5, and 2%	280:311	The milk samples with RGE added at 0.5, 1, 1.5, and 2% were analyzed during storage at 4°C.
25465553	0	70	theme	milk	67:70	arg1	properties					53:62	Physicochemical and antioxidant properties	21:62	Physicochemical and antioxidant properties of milk supplemented with red ginseng extract	21:108	Short communication: Physicochemical and antioxidant properties of milk supplemented with red ginseng extract.
25465553	9	71	theme	2	1163:1163	arg1	%					1164:1164	%	1164:1164	%	1164:1164	Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin.
25465553	1	72	theme	red	149:151	arg1	RGE					170:172	RGE	170:172	RGE	170:172	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	1	72	theme	red	149:151	arg1	extract					161:167	red ginseng extract	149:167	red ginseng extract (RGE)	149:173	This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk.
25465553	6	73	theme	*	819:819	arg1	value					821:825	L* value	818:825	L* value	818:825	Also, pH and L* value decreased.
25465553	4	74	theme	bleaching	561:569	arg1	method					542:547	the 2,2-diphenyl-1-picrylhydrazyl method	508:547	the 2,2-diphenyl-1-picrylhydrazyl method	508:547	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
25465553	4	74	theme	bleaching	561:569	arg1	assay					571:575	β-carotene bleaching assay	550:575	β-carotene bleaching assay	550:575	The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, β-carotene bleaching assay, and ferric thiocyanate assay.
24608968	0	0	theme	strain	86:91	arg1	O16					108:110	Aeromonas veronii strain Bs19, serotype O16	68:110	O16	108:110	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	5	1	theme	R-form	1057:1062	arg1	species					1068:1074	SR- and R-form LPS species	1049:1074	SR- and R-form LPS species	1049:1074	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	2	2	theme	abundant	329:336	arg1	glycoforms					342:351	the most abundant LPS glycoforms	320:351	the most abundant LPS glycoforms	320:351	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	3	3	theme	1→3	847:849	arg1	-α-D-GalpNAc-					881:893	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	2	4	theme	AraN	467:470	arg1	residue					472:478	an AraN residue	464:478	an AraN residue as a non-stoichiometric substituent	464:514	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	0	5	from	studies	11:17	arg1	pathogen					59:66	the fish pathogen	50:66	the fish pathogen	50:66	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	5	6	dep	=	1097:1097	arg1	u					1106:1106	698.27 u	1099:1106	698.27 u	1099:1106	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	3	7	theme	¹³C	639:641	arg1	spectroscopy					647:658	¹³C NMR spectroscopy	639:658	¹³C NMR spectroscopy	639:658	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	5	8	theme	species	1068:1074	arg1	peaks					1040:1044	mass peaks	1035:1044	mass peaks	1035:1044	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	6	9	theme	biological	1392:1401	arg1	unit					1413:1416	the biological repeating unit	1388:1416	the biological repeating unit	1388:1416	Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
24608968	0	10	dep	O16	108:110	arg1	serotype					99:106	Aeromonas veronii strain Bs19, serotype O16	68:110	serotype	99:106	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	0	10	dep	O16	108:110	arg1	studies					11:17	Structural studies	0:17	Structural studies of the lipopolysaccharide from the fish pathogen	0:66	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	3	11	theme	-α-D-Quip3NAc-	832:845	arg1	-α-D-GalpNAc-					881:893	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	12	theme	tetrasaccharide	777:791	arg1	units					803:807	tetrasaccharide repeating units	777:807	tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	777:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	0	13	theme	Bs19	93:96	arg1	O16					108:110	Aeromonas veronii strain Bs19, serotype O16	68:110	O16	108:110	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	5	14	theme	mass	1035:1038	arg1	peaks					1040:1044	mass peaks	1035:1044	mass peaks	1035:1044	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	5	15	theme	molecular	1149:1157	arg1	mass					1159:1162	the calculated molecular mass	1134:1162	the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O)	1134:1217	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	6	16	from	sequence	1277:1284	arg1	OPS					1315:1317	the OPS	1311:1317	the OPS	1311:1317	Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
24608968	6	17	theme	fragmentation	1241:1253	arg1	spectra					1255:1261	unspecific fragmentation spectra	1230:1261	unspecific fragmentation spectra	1230:1261	Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
24608968	3	18	theme	repeating	793:801	arg1	units					803:807	tetrasaccharide repeating units	777:807	tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	777:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	19	theme	O-specific	710:719	arg1	OPS					737:739	OPS	737:739	OPS	737:739	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	19	theme	O-specific	710:719	arg1	polysaccharide					721:734	the O-specific polysaccharide	706:734	the O-specific polysaccharide (OPS) of A. veronii Bs19	706:759	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	20	dep	-α-D-GalpNAc-	881:893	arg1	1→3					877:879	1→3	877:879	1→3	877:879	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	1	21	theme	NMR	155:157	arg1	spectroscopy					159:170	NMR spectroscopy	155:170	NMR spectroscopy	155:170	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	2	22	theme	non-stoichiometric	485:502	arg1	substituent					504:514	a non-stoichiometric substituent	483:514	a non-stoichiometric substituent	483:514	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	6	23	from	OPS	1315:1317	arg1	sequence					1277:1284	the sequence	1273:1284	the sequence of the sugar residues in the OPS	1273:1317	Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
24608968	0	24	theme	Structural	0:9	arg1	studies					11:17	Structural studies	0:17	Structural studies of the lipopolysaccharide from the fish pathogen	0:66	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	3	25	dep	A.	745:746	arg1	veronii					748:754	veronii	748:754	veronii	748:754	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	2	26	theme	bisphosphorylated	423:439	arg1	disaccharide					446:457	a bisphosphorylated GlcN disaccharide	421:457	a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent	421:514	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	5	27	theme	SR-	1049:1051	arg1	species					1068:1074	SR- and R-form LPS species	1049:1074	SR- and R-form LPS species	1049:1074	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	3	28	dep	methods	674:680	arg1	used					682:685	used	682:685	used	682:685	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	28	dep	methods	674:680	arg1	revealed					692:699	revealed	692:699	revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	692:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	29	theme	-α-L-Rhap-	851:860	arg1	-α-D-GalpNAc-					881:893	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	2	30	theme	tetra-acylated	358:371	arg1	species					398:404	tetra-acylated or hexa-acylated lipid A species	358:404	species	398:404	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	1	31	dep	Aeromonas	254:262	arg1	veronii					264:270	veronii	264:270	veronii	264:270	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	1	32	theme	Aeromonas	254:262	arg1	Bs19					279:282	Aeromonas veronii strain Bs19	254:282	Aeromonas veronii strain Bs19	254:282	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	1	32	theme	Aeromonas	254:262	arg1	O16					294:296	serotype O16	285:296	serotype O16	285:296	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	3	33	theme	structure	818:826	arg1	-α-D-GalpNAc-					881:893	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	5	34	theme	LPS	1007:1009	arg1	preparations					1011:1022	the LPS preparations	1003:1022	the LPS preparations	1003:1022	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	5	35	theme	=	1097:1097	arg1	value					1111:1115	a value	1109:1115	a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O)	1109:1217	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	5	35	theme	=	1097:1097	arg1	Δm					1094:1095	Δm	1094:1095	Δm = 698.27 u	1094:1106	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	5	36	theme	ESI	976:978	arg1	MS					987:988	The charge-deconvoluted ESI FT-ICR MS	952:988	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations	952:1022	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	0	37	dep	Aeromonas	68:76	arg1	veronii					78:84	veronii	78:84	veronii	78:84	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	6	38	theme	elucidated	1350:1359	arg1	structure					1361:1369	the elucidated structure	1346:1369	the elucidated structure	1346:1369	Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
24608968	1	39	theme	strain	272:277	arg1	Bs19					279:282	Aeromonas veronii strain Bs19	254:282	Aeromonas veronii strain Bs19	254:282	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	1	39	theme	strain	272:277	arg1	O16					294:296	serotype O16	285:296	serotype O16	285:296	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	2	40	gly	glycoforms	342:351	arg1	LPS					338:340	the most abundant LPS glycoforms	320:351	the most abundant LPS glycoforms	320:351	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	2	41	contain	have	353:356	arg2	species					398:404	tetra-acylated or hexa-acylated lipid A species	358:404	species	398:404	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	2	41	contain	have	353:356	arg1	glycoforms					342:351	the most abundant LPS glycoforms	320:351	the most abundant LPS glycoforms	320:351	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	2	42	theme	core	523:526	arg1	oligosaccharide					528:542	a core oligosaccharide	521:542	a core oligosaccharide	521:542	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	3	43	theme	NMR	643:645	arg1	spectroscopy					647:658	¹³C NMR spectroscopy	639:658	¹³C NMR spectroscopy	639:658	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	44	theme	-β-D-Galp-	866:875	arg1	-α-D-GalpNAc-					881:893	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	2	45	theme	A	396:396	arg1	species					398:404	tetra-acylated or hexa-acylated lipid A species	358:404	species	398:404	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	0	46	theme	lipopolysaccharide	26:43	arg1	studies					11:17	Structural studies	0:17	Structural studies of the lipopolysaccharide from the fish pathogen	0:66	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	4	47	theme	mass	933:936	arg1	spectrometry					938:949	mass spectrometry	933:949	mass spectrometry	933:949	This composition was confirmed by mass spectrometry.
24608968	6	48	theme	unspecific	1230:1239	arg1	spectra					1255:1261	unspecific fragmentation spectra	1230:1261	unspecific fragmentation spectra	1230:1261	Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
24608968	5	49	theme	unit	1185:1188	arg1	mass					1159:1162	the calculated molecular mass	1134:1162	the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O)	1134:1217	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	5	50	theme	calculated	1138:1147	arg1	mass					1159:1162	the calculated molecular mass	1134:1162	the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O)	1134:1217	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	2	51	theme	lipid	390:394	arg1	species					398:404	tetra-acylated or hexa-acylated lipid A species	358:404	species	398:404	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	0	52	theme	fish	54:57	arg1	pathogen					59:66	the fish pathogen	50:66	the fish pathogen	50:66	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	1	53	theme	serotype	285:292	arg1	Bs19					279:282	Aeromonas veronii strain Bs19	254:282	Aeromonas veronii strain Bs19	254:282	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	1	53	theme	serotype	285:292	arg1	O16					294:296	serotype O16	285:296	serotype O16	285:296	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	0	54	from	pathogen	59:66	arg1	studies					11:17	Structural studies	0:17	Structural studies of the lipopolysaccharide from the fish pathogen	0:66	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	0	54	from	pathogen	59:66	arg1	lipopolysaccharide					26:43	the lipopolysaccharide	22:43	the lipopolysaccharide from the fish pathogen	22:66	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	5	55	theme	charge-deconvoluted	956:974	arg1	MS					987:988	The charge-deconvoluted ESI FT-ICR MS	952:988	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations	952:1022	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	5	56	theme	LPS	1064:1066	arg1	species					1068:1074	SR- and R-form LPS species	1049:1074	SR- and R-form LPS species	1049:1074	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	2	57	theme	GlcN	441:444	arg1	disaccharide					446:457	a bisphosphorylated GlcN disaccharide	421:457	a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent	421:514	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	2	58	theme	hexa-acylated	376:388	arg1	species					398:404	tetra-acylated or hexa-acylated lipid A species	358:404	species	398:404	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	6	59	theme	residues	1299:1306	arg1	sequence					1277:1284	the sequence	1273:1284	the sequence of the sugar residues in the OPS	1273:1317	Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
24608968	5	60	theme	FT-ICR	980:985	arg1	MS					987:988	The charge-deconvoluted ESI FT-ICR MS	952:988	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations	952:1022	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	3	61	theme	methylation	587:597	arg1	analysis					599:606	methylation analysis	587:606	methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy	587:658	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	61	theme	methylation	587:597	arg1	methods					674:680	the main methods	665:680	the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	665:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	61	theme	methylation	587:597	arg1	Sugar					577:581	Sugar	577:581	Sugar	577:581	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	62	with	units	803:807	arg1	-α-D-GalpNAc-					881:893	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	1	63	theme	Chemical	113:120	arg1	analyses					122:129	Chemical analyses	113:129	Chemical analyses	113:129	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	5	64	theme	repeating	1175:1183	arg1	unit					1185:1188	one OPS repeating unit	1167:1188	one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O)	1167:1217	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	5	64	theme	repeating	1175:1183	arg1	6dHexNAc6dHexHexHexNAc-H₂O					1191:1216	6dHexNAc6dHexHexHexNAc-H₂O	1191:1216	6dHexNAc6dHexHexHexNAc-H₂O	1191:1216	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	3	65	theme	1D	622:623	arg1	¹H					632:633	1D and 2D ¹H	622:633	¹H	632:633	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	66	theme	main	669:672	arg1	analysis					599:606	methylation analysis	587:606	methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy	587:658	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	66	theme	main	669:672	arg1	methods					674:680	the main methods	665:680	the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	665:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	66	theme	main	669:672	arg1	Sugar					577:581	Sugar	577:581	Sugar	577:581	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	67	theme	→4	829:830	arg1	-α-D-GalpNAc-					881:893	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→	814:896	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	68	theme	A.	745:746	arg1	Bs19					756:759	A. veronii Bs19	745:759	A. veronii Bs19	745:759	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	69	theme	2D	629:630	arg1	¹H					632:633	1D and 2D ¹H	622:633	¹H	632:633	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	2	70	with	disaccharide	446:457	arg1	residue					472:478	an AraN residue	464:478	an AraN residue as a non-stoichiometric substituent	464:514	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	6	71	theme	repeating	1403:1411	arg1	unit					1413:1416	the biological repeating unit	1388:1416	the biological repeating unit	1388:1416	Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
24608968	0	72	theme	Aeromonas	68:76	arg1	O16					108:110	Aeromonas veronii strain Bs19, serotype O16	68:110	O16	108:110	Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16.
24608968	5	73	theme	OPS	1171:1173	arg1	unit					1185:1188	one OPS repeating unit	1167:1188	one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O)	1167:1217	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	5	73	theme	OPS	1171:1173	arg1	6dHexNAc6dHexHexHexNAc-H₂O					1191:1216	6dHexNAc6dHexHexHexNAc-H₂O	1191:1216	6dHexNAc6dHexHexHexNAc-H₂O	1191:1216	The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O).
24608968	6	74	theme	sugar	1293:1297	arg1	residues					1299:1306	the sugar residues	1289:1306	the sugar residues in the OPS	1289:1317	Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
24608968	1	75	theme	mass	132:135	arg1	spectrometry					137:148	mass spectrometry	132:148	mass spectrometry	132:148	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	2	76	with	oligosaccharide	528:542	arg1	residue					472:478	an AraN residue	464:478	an AraN residue as a non-stoichiometric substituent	464:514	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	1	77	theme	lipopolysaccharide	215:232	arg1	structure					198:206	the structure	194:206	the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16	194:296	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	6	78	from	residues	1299:1306	arg1	OPS					1315:1317	the OPS	1311:1317	the OPS	1311:1317	Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.
24608968	1	79	attach	isolated	240:247	arg1	Bs19					279:282	Aeromonas veronii strain Bs19	254:282	Aeromonas veronii strain Bs19	254:282	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	1	79	attach	isolated	240:247	arg1	O16					294:296	serotype O16	285:296	serotype O16	285:296	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	1	79	attach	isolated	240:247	arg2	LPS					235:237	LPS	235:237	LPS	235:237	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	1	79	attach	isolated	240:247	arg2	lipopolysaccharide					215:232	the lipopolysaccharide	211:232	the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16	211:296	Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16.
24608968	2	80	theme	LPS	338:340	arg1	glycoforms					342:351	the most abundant LPS glycoforms	320:351	the most abundant LPS glycoforms	320:351	ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁.
24608968	3	81	theme	Bs19	756:759	arg1	OPS					737:739	OPS	737:739	OPS	737:739	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
24608968	3	81	theme	Bs19	756:759	arg1	polysaccharide					721:734	the O-specific polysaccharide	706:734	the O-specific polysaccharide (OPS) of A. veronii Bs19	706:759	Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→.
27498308	8	0	theme	PEDOT-HA/PLLA	1206:1218	arg1	films					1220:1224	PEDOT-HA/PLLA films	1206:1224	PEDOT-HA/PLLA films modified with laminin (LN)	1206:1251	PEDOT-HA/PLLA films modified with laminin (LN) also exhibited an efficiently elongated cell morphology observed by fluorescent microscope and metallographic microscope.
27498308	10	1	theme	other	1773:1777	arg1	groups					1782:1787	other ES groups	1773:1787	other ES groups	1773:1787	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	10	2	from	expression	1668:1677	arg1	cells					1748:1752	PC12 cells	1743:1752	PC12 cells	1743:1752	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	6	3	theme	PEDOT-HA/PLLA	856:868	arg1	films					870:874	PEDOT-HA/PLLA films	856:874	PEDOT-HA/PLLA films	856:874	PEDOT-HA/PLLA films showed lower surface contact angle and faster degradation degree compared with PLLA films.
27498308	11	4	theme	conductive	1920:1929	arg1	candidate					1971:1979	an attractive candidate	1957:1979	an attractive candidate with ES for enhancing nerve regeneration	1957:2020	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	11	4	theme	conductive	1920:1929	arg1	film					1945:1948	the conductive PEDOT-HA/PLLA film	1916:1948	the conductive PEDOT-HA/PLLA film	1916:1948	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	9	5	theme	neurite	1512:1518	arg1	outgrowth					1520:1528	neurite outgrowth	1512:1528	neurite outgrowth of PC12 cells	1512:1542	Furthermore, PEDOT-HA/PLLA films were subjected to different current intensity to elucidate the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells.
27498308	6	6	theme	PLLA	955:958	arg1	films					960:964	PLLA films	955:964	PLLA films	955:964	PEDOT-HA/PLLA films showed lower surface contact angle and faster degradation degree compared with PLLA films.
27498308	4	7	theme	Raman	676:680	arg1	analysis					690:697	FTIR, Raman and EDX analysis	670:697	FTIR, Raman and EDX analysis	670:697	FTIR, Raman and EDX analysis demonstrated that HA was successfully doped into PEDOT particles.
27498308	4	8	theme	EDX	686:688	arg1	analysis					690:697	FTIR, Raman and EDX analysis	670:697	FTIR, Raman and EDX analysis	670:697	FTIR, Raman and EDX analysis demonstrated that HA was successfully doped into PEDOT particles.
27498308	7	9	theme	viability	1147:1155	arg1	surface					1107:1113	the surface	1103:1113	the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay	1103:1176	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	9	10	theme	cells	1538:1542	arg1	outgrowth					1520:1528	neurite outgrowth	1512:1528	neurite outgrowth of PC12 cells	1512:1542	Furthermore, PEDOT-HA/PLLA films were subjected to different current intensity to elucidate the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells.
27498308	2	11	theme	composite	546:554	arg1	films					556:560	conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films	499:560	conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films	499:560	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	11	12	theme	PEDOT-HA/PLLA	1931:1943	arg1	candidate					1971:1979	an attractive candidate	1957:1979	an attractive candidate with ES for enhancing nerve regeneration	1957:2020	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	11	12	theme	PEDOT-HA/PLLA	1931:1943	arg1	film					1945:1948	the conductive PEDOT-HA/PLLA film	1916:1948	the conductive PEDOT-HA/PLLA film	1916:1948	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	4	13	theme	FTIR	670:673	arg1	analysis					690:697	FTIR, Raman and EDX analysis	670:697	FTIR, Raman and EDX analysis	670:697	FTIR, Raman and EDX analysis demonstrated that HA was successfully doped into PEDOT particles.
27498308	7	14	theme	PEDOT-HA/PLLA	1118:1130	arg1	films					1132:1136	PEDOT-HA/PLLA films	1118:1136	PEDOT-HA/PLLA films	1118:1136	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	11	15	theme	nerve	2025:2029	arg1	engineering					2038:2048	nerve tissue engineering	2025:2048	nerve tissue engineering	2025:2048	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	8	16	theme	elongated	1283:1291	arg1	morphology					1298:1307	an efficiently elongated cell morphology	1268:1307	an efficiently elongated cell morphology observed by fluorescent microscope and metallographic microscope	1268:1372	PEDOT-HA/PLLA films modified with laminin (LN) also exhibited an efficiently elongated cell morphology observed by fluorescent microscope and metallographic microscope.
27498308	1	17	mod	modified	263:270	arg1	PEDOT					205:209	PEDOT	205:209	PEDOT	205:209	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	1	17	mod	modified	263:270	arg1	3,4-ethylenedioxythiophene					177:202	Poly 3,4-ethylenedioxythiophene	172:202	Poly 3,4-ethylenedioxythiophene (PEDOT)	172:210	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	1	17	mod	modified	263:270	arg3	process					284:290	chemical process	275:290	chemical process as biocompatible conductive polymer for biomedical applications	275:354	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	1	17	mod	modified	263:270	arg1	derivative					229:238	a polythiophene derivative	213:238	a polythiophene derivative	213:238	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	10	18	theme	growth-associated	1682:1698	arg1	protein					1700:1706	growth-associated protein	1682:1706	growth-associated protein (GAP43)	1682:1714	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	10	18	theme	growth-associated	1682:1698	arg1	GAP43					1709:1713	GAP43	1709:1713	GAP43	1709:1713	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	11	19	theme	cell	1870:1873	arg1	growth					1875:1880	cell growth	1870:1880	cell growth	1870:1880	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	0	20	theme	biomedical	148:157	arg1	application					159:169	biomedical application	148:169	biomedical application	148:169	Fabrication and characterization of conductive poly (3,4-ethylenedioxythiophene) doped with hyaluronic acid/poly (l-lactic acid) composite film for biomedical application.
27498308	3	21	theme	physicochemical	581:595	arg1	characteristics					597:611	The physicochemical characteristics	577:611	The physicochemical characteristics	577:611	The physicochemical characteristics and biocompatibility of films were further investigated.
27498308	11	22	with	candidate	1971:1979	arg1	ES					1986:1987	ES	1986:1987	ES	1986:1987	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	7	23	theme	PC12	1064:1067	arg1	cells					1070:1074	neuron-like pheochromocytoma (PC12) cells	1034:1074	neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay	1034:1176	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	2	24	theme	PEDOT-HA/poly	510:522	arg1	films					556:560	conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films	499:560	conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films	499:560	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	5	25	contain	had	815:817	arg2	stability					845:853	favorable electrochemical stability	819:853	favorable electrochemical stability	819:853	Cyclic voltammograms indicated PEDOT-HA particles had favorable electrochemical stability.
27498308	5	25	contain	had	815:817	arg1	voltammograms					772:784	Cyclic voltammograms	765:784	Cyclic voltammograms indicated PEDOT-HA particles	765:813	Cyclic voltammograms indicated PEDOT-HA particles had favorable electrochemical stability.
27498308	7	26	theme	pheochromocytoma	1046:1061	arg1	cells					1070:1074	neuron-like pheochromocytoma (PC12) cells	1034:1074	neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay	1034:1176	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	11	27	from	candidate	1971:1979	arg1	engineering					2038:2048	nerve tissue engineering	2025:2048	nerve tissue engineering	2025:2048	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	9	28	from	effect	1471:1476	arg1	outgrowth					1520:1528	neurite outgrowth	1512:1528	neurite outgrowth of PC12 cells	1512:1542	Furthermore, PEDOT-HA/PLLA films were subjected to different current intensity to elucidate the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells.
27498308	1	29	theme	Poly	172:175	arg1	PEDOT					205:209	PEDOT	205:209	PEDOT	205:209	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	1	29	theme	Poly	172:175	arg1	3,4-ethylenedioxythiophene					177:202	Poly 3,4-ethylenedioxythiophene	172:202	Poly 3,4-ethylenedioxythiophene (PEDOT)	172:210	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	1	29	theme	Poly	172:175	arg1	derivative					229:238	a polythiophene derivative	213:238	a polythiophene derivative	213:238	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	7	30	contain	had	1178:1180	arg2	increase					1196:1203	a significant increase	1182:1203	a significant increase	1182:1203	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	7	30	contain	had	1178:1180	arg1	cells					1070:1074	neuron-like pheochromocytoma (PC12) cells	1034:1074	neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay	1034:1176	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	11	31	theme	PEDOT-HA/PLLA	1817:1829	arg1	film					1831:1834	PEDOT-HA/PLLA film	1817:1834	PEDOT-HA/PLLA film combined with ES	1817:1851	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	0	32	theme	acid/poly	103:111	arg1	film					139:142	hyaluronic acid/poly (l-lactic acid) composite film	92:142	hyaluronic acid/poly (l-lactic acid) composite film for biomedical application	92:169	Fabrication and characterization of conductive poly (3,4-ethylenedioxythiophene) doped with hyaluronic acid/poly (l-lactic acid) composite film for biomedical application.
27498308	9	33	theme	PEDOT-HA/PLLA	1388:1400	arg1	films					1402:1406	PEDOT-HA/PLLA films	1388:1406	PEDOT-HA/PLLA films	1388:1406	Furthermore, PEDOT-HA/PLLA films were subjected to different current intensity to elucidate the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells.
27498308	7	34	theme	films	1016:1020	arg1	test					994:997	the cytotoxicity test	977:997	the cytotoxicity test of PEDOT-HA/PLLA films	977:1020	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	2	35	theme	l-lactic	524:531	arg1	PEDOT-HA/poly					510:522	conductive PEDOT-HA/poly	499:522	conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films	499:560	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	2	35	theme	l-lactic	524:531	arg1	acid					533:536	l-lactic acid	524:536	l-lactic acid	524:536	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	11	36	theme	attractive	1960:1969	arg1	candidate					1971:1979	an attractive candidate	1957:1979	an attractive candidate with ES for enhancing nerve regeneration	1957:2020	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	11	36	theme	attractive	1960:1969	arg1	film					1945:1948	the conductive PEDOT-HA/PLLA film	1916:1948	the conductive PEDOT-HA/PLLA film	1916:1948	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	5	37	theme	Cyclic	765:770	arg1	voltammograms					772:784	Cyclic voltammograms	765:784	Cyclic voltammograms indicated PEDOT-HA particles	765:813	Cyclic voltammograms indicated PEDOT-HA particles had favorable electrochemical stability.
27498308	1	38	theme	chemical	275:282	arg1	process					284:290	chemical process	275:290	chemical process as biocompatible conductive polymer for biomedical applications	275:354	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	7	39	theme	cytotoxicity	981:992	arg1	test					994:997	the cytotoxicity test	977:997	the cytotoxicity test of PEDOT-HA/PLLA films	977:1020	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	10	40	theme	PC12	1743:1746	arg1	cells					1748:1752	PC12 cells	1743:1752	PC12 cells	1743:1752	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	9	41	theme	current	1436:1442	arg1	intensity					1444:1452	different current intensity	1426:1452	different current intensity	1426:1452	Furthermore, PEDOT-HA/PLLA films were subjected to different current intensity to elucidate the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells.
27498308	2	42	theme	polymerization	478:491	arg1	method					449:454	the method	445:454	the method of chemical oxidative polymerization	445:491	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	2	43	theme	PEDOT	405:409	arg1	nanoparticles					411:423	novel hyaluronic acid (HA)-doped PEDOT nanoparticles	372:423	novel hyaluronic acid (HA)-doped PEDOT nanoparticles	372:423	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	0	44	theme	conductive	36:45	arg1	poly					47:50	conductive poly	36:50	conductive poly (3,4-ethylenedioxythiophene)	36:79	Fabrication and characterization of conductive poly (3,4-ethylenedioxythiophene) doped with hyaluronic acid/poly (l-lactic acid) composite film for biomedical application.
27498308	0	44	theme	conductive	36:45	arg1	3,4-ethylenedioxythiophene					53:78	3,4-ethylenedioxythiophene	53:78	3,4-ethylenedioxythiophene	53:78	Fabrication and characterization of conductive poly (3,4-ethylenedioxythiophene) doped with hyaluronic acid/poly (l-lactic acid) composite film for biomedical application.
27498308	1	45	theme	conductive	309:318	arg1	polymer					320:326	biocompatible conductive polymer	295:326	biocompatible conductive polymer for biomedical applications	295:354	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	5	46	theme	favorable	819:827	arg1	stability					845:853	favorable electrochemical stability	819:853	favorable electrochemical stability	819:853	Cyclic voltammograms indicated PEDOT-HA particles had favorable electrochemical stability.
27498308	6	47	theme	faster	915:920	arg1	degradation					922:932	faster degradation	915:932	faster degradation	915:932	PEDOT-HA/PLLA films showed lower surface contact angle and faster degradation degree compared with PLLA films.
27498308	2	48	theme	chemical	459:466	arg1	polymerization					478:491	chemical oxidative polymerization	459:491	chemical oxidative polymerization	459:491	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	6	49	theme	angle	905:909	arg1	degree					934:939	lower surface contact angle and faster degradation degree	883:939	lower surface contact angle and faster degradation degree	883:939	PEDOT-HA/PLLA films showed lower surface contact angle and faster degradation degree compared with PLLA films.
27498308	10	50	theme	neurite	1585:1591	arg1	outgrowth					1593:1601	neurite outgrowth	1585:1601	neurite outgrowth	1585:1601	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	6	51	theme	surface	889:895	arg1	angle					905:909	surface contact angle	889:909	surface contact angle	889:909	PEDOT-HA/PLLA films showed lower surface contact angle and faster degradation degree compared with PLLA films.
27498308	2	52	theme	-doped	398:403	arg1	nanoparticles					411:423	novel hyaluronic acid (HA)-doped PEDOT nanoparticles	372:423	novel hyaluronic acid (HA)-doped PEDOT nanoparticles	372:423	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	2	53	theme	hyaluronic	378:387	arg1	HA					395:396	HA	395:396	HA	395:396	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	2	53	theme	hyaluronic	378:387	arg1	acid					389:392	novel hyaluronic acid	372:392	novel hyaluronic acid (HA)-doped PEDOT nanoparticles	372:423	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	9	54	theme	stimulation	1492:1502	arg1	effect					1471:1476	the effect	1467:1476	the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells	1467:1542	Furthermore, PEDOT-HA/PLLA films were subjected to different current intensity to elucidate the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells.
27498308	5	55	theme	electrochemical	829:843	arg1	stability					845:853	favorable electrochemical stability	819:853	favorable electrochemical stability	819:853	Cyclic voltammograms indicated PEDOT-HA particles had favorable electrochemical stability.
27498308	10	56	theme	average	1611:1617	arg1	length					1625:1630	an average value length	1608:1630	an average value length of 122 ± 5 μm	1608:1644	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	5	57	theme	PEDOT-HA	796:803	arg1	particles					805:813	PEDOT-HA particles	796:813	PEDOT-HA particles	796:813	Cyclic voltammograms indicated PEDOT-HA particles had favorable electrochemical stability.
27498308	10	58	theme	ES	1779:1780	arg1	groups					1782:1787	other ES groups	1773:1787	other ES groups	1773:1787	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	7	59	theme	MTT	1168:1170	arg1	assay					1172:1176	MTT assay	1168:1176	MTT assay	1168:1176	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	10	60	theme	122 ± 5 μm	1635:1644	arg1	length					1625:1630	an average value length	1608:1630	an average value length of 122 ± 5 μm	1608:1644	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	8	61	theme	cell	1293:1296	arg1	morphology					1298:1307	an efficiently elongated cell morphology	1268:1307	an efficiently elongated cell morphology observed by fluorescent microscope and metallographic microscope	1268:1372	PEDOT-HA/PLLA films modified with laminin (LN) also exhibited an efficiently elongated cell morphology observed by fluorescent microscope and metallographic microscope.
27498308	7	62	theme	cell	1142:1145	arg1	viability					1147:1155	cell viability	1142:1155	cell viability	1142:1155	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	9	63	theme	PC12	1533:1536	arg1	cells					1538:1542	PC12 cells	1533:1542	PC12 cells	1533:1542	Furthermore, PEDOT-HA/PLLA films were subjected to different current intensity to elucidate the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells.
27498308	10	64	theme	mRNA	1663:1666	arg1	expression					1668:1677	the mRNA expression	1659:1677	the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells	1659:1752	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	7	65	theme	films	1132:1136	arg1	surface					1107:1113	the surface	1103:1113	the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay	1103:1176	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	8	66	theme	fluorescent	1321:1331	arg1	microscope					1333:1342	fluorescent microscope	1321:1342	fluorescent microscope	1321:1342	PEDOT-HA/PLLA films modified with laminin (LN) also exhibited an efficiently elongated cell morphology observed by fluorescent microscope and metallographic microscope.
27498308	1	67	theme	polythiophene	215:227	arg1	3,4-ethylenedioxythiophene					177:202	Poly 3,4-ethylenedioxythiophene	172:202	Poly 3,4-ethylenedioxythiophene (PEDOT)	172:210	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	1	67	theme	polythiophene	215:227	arg1	derivative					229:238	a polythiophene derivative	213:238	a polythiophene derivative	213:238	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	8	68	theme	metallographic	1348:1361	arg1	microscope					1363:1372	metallographic microscope	1348:1372	metallographic microscope	1348:1372	PEDOT-HA/PLLA films modified with laminin (LN) also exhibited an efficiently elongated cell morphology observed by fluorescent microscope and metallographic microscope.
27498308	10	69	theme	protein	1700:1706	arg1	expression					1668:1677	the mRNA expression	1659:1677	the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells	1659:1752	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	11	70	theme	neurite	1886:1892	arg1	outgrowth					1894:1902	neurite outgrowth	1886:1902	neurite outgrowth	1886:1902	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	11	71	theme	nerve	2003:2007	arg1	regeneration					2009:2020	nerve regeneration	2003:2020	nerve regeneration	2003:2020	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	0	72	theme	composite	129:137	arg1	film					139:142	hyaluronic acid/poly (l-lactic acid) composite film	92:142	hyaluronic acid/poly (l-lactic acid) composite film for biomedical application	92:169	Fabrication and characterization of conductive poly (3,4-ethylenedioxythiophene) doped with hyaluronic acid/poly (l-lactic acid) composite film for biomedical application.
27498308	3	73	theme	films	637:641	arg1	characteristics					597:611	The physicochemical characteristics	577:611	The physicochemical characteristics	577:611	The physicochemical characteristics and biocompatibility of films were further investigated.
27498308	3	73	theme	films	637:641	arg1	biocompatibility					617:632	biocompatibility	617:632	biocompatibility of films	617:641	The physicochemical characteristics and biocompatibility of films were further investigated.
27498308	11	74	theme	tissue	2031:2036	arg1	engineering					2038:2048	nerve tissue engineering	2025:2048	nerve tissue engineering	2025:2048	These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering.
27498308	2	75	theme	conductive	499:508	arg1	PEDOT-HA/poly					510:522	conductive PEDOT-HA/poly	499:522	conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films	499:560	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	2	75	theme	conductive	499:508	arg1	acid					533:536	l-lactic acid	524:536	l-lactic acid	524:536	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	2	75	theme	conductive	499:508	arg1	PLLA					540:543	PLLA	540:543	PLLA	540:543	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	0	76	theme	hyaluronic	92:101	arg1	film					139:142	hyaluronic acid/poly (l-lactic acid) composite film	92:142	hyaluronic acid/poly (l-lactic acid) composite film for biomedical application	92:169	Fabrication and characterization of conductive poly (3,4-ethylenedioxythiophene) doped with hyaluronic acid/poly (l-lactic acid) composite film for biomedical application.
27498308	7	77	theme	neuron-like	1034:1044	arg1	cells					1070:1074	neuron-like pheochromocytoma (PC12) cells	1034:1074	neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay	1034:1176	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	7	78	dep	cells	1070:1074	arg1	adhered					1076:1082	adhered	1076:1082	adhered	1076:1082	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	7	78	dep	cells	1070:1074	arg1	spread					1088:1093	spread	1088:1093	spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay	1088:1176	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	0	79	theme	acid	123:126	arg1	film					139:142	hyaluronic acid/poly (l-lactic acid) composite film	92:142	hyaluronic acid/poly (l-lactic acid) composite film for biomedical application	92:169	Fabrication and characterization of conductive poly (3,4-ethylenedioxythiophene) doped with hyaluronic acid/poly (l-lactic acid) composite film for biomedical application.
27498308	10	80	theme	synaptophysin	1720:1732	arg1	expression					1668:1677	the mRNA expression	1659:1677	the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells	1659:1752	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	10	81	dep	ES	1545:1546	arg1	2 h					1557:1559	2 h	1557:1559	2 h	1557:1559	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	10	81	dep	ES	1545:1546	arg1	0.5 mA					1549:1554	0.5 mA	1549:1554	0.5 mA	1549:1554	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	7	82	theme	PEDOT-HA/PLLA	1002:1014	arg1	films					1016:1020	PEDOT-HA/PLLA films	1002:1020	PEDOT-HA/PLLA films	1002:1020	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	4	83	theme	PEDOT	748:752	arg1	particles					754:762	PEDOT particles	748:762	PEDOT particles	748:762	FTIR, Raman and EDX analysis demonstrated that HA was successfully doped into PEDOT particles.
27498308	2	84	theme	acid	389:392	arg1	nanoparticles					411:423	novel hyaluronic acid (HA)-doped PEDOT nanoparticles	372:423	novel hyaluronic acid (HA)-doped PEDOT nanoparticles	372:423	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	9	85	theme	different	1426:1434	arg1	intensity					1444:1452	different current intensity	1426:1452	different current intensity	1426:1452	Furthermore, PEDOT-HA/PLLA films were subjected to different current intensity to elucidate the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells.
27498308	0	86	theme	poly	47:50	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of conductive poly (3,4-ethylenedioxythiophene) doped with hyaluronic acid/poly (l-lactic acid) composite film for biomedical application.
27498308	0	86	theme	poly	47:50	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of conductive poly (3,4-ethylenedioxythiophene) doped with hyaluronic acid/poly (l-lactic acid) composite film for biomedical application.
27498308	1	87	theme	biocompatible	295:307	arg1	polymer					320:326	biocompatible conductive polymer	295:326	biocompatible conductive polymer for biomedical applications	295:354	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	6	88	theme	degradation	922:932	arg1	degree					934:939	lower surface contact angle and faster degradation degree	883:939	lower surface contact angle and faster degradation degree	883:939	PEDOT-HA/PLLA films showed lower surface contact angle and faster degradation degree compared with PLLA films.
27498308	2	89	theme	oxidative	468:476	arg1	polymerization					478:491	chemical oxidative polymerization	459:491	chemical oxidative polymerization	459:491	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	0	90	theme	l-lactic	114:121	arg1	acid					123:126	l-lactic acid	114:126	hyaluronic acid/poly (l-lactic acid) composite film for biomedical application	92:169	Fabrication and characterization of conductive poly (3,4-ethylenedioxythiophene) doped with hyaluronic acid/poly (l-lactic acid) composite film for biomedical application.
27498308	7	91	theme	significant	1184:1194	arg1	increase					1196:1203	a significant increase	1182:1203	a significant increase	1182:1203	Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by MTT assay had a significant increase.
27498308	1	92	theme	biomedical	332:341	arg1	applications					343:354	biomedical applications	332:354	biomedical applications	332:354	Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications.
27498308	6	93	theme	contact	897:903	arg1	angle					905:909	surface contact angle	889:909	surface contact angle	889:909	PEDOT-HA/PLLA films showed lower surface contact angle and faster degradation degree compared with PLLA films.
27498308	9	94	theme	electrical	1481:1490	arg1	stimulation					1492:1502	electrical stimulation	1481:1502	electrical stimulation (ES)	1481:1507	Furthermore, PEDOT-HA/PLLA films were subjected to different current intensity to elucidate the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells.
27498308	9	94	theme	electrical	1481:1490	arg1	ES					1505:1506	ES	1505:1506	ES	1505:1506	Furthermore, PEDOT-HA/PLLA films were subjected to different current intensity to elucidate the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells.
27498308	6	95	theme	lower	883:887	arg1	degree					934:939	lower surface contact angle and faster degradation degree	883:939	lower surface contact angle and faster degradation degree	883:939	PEDOT-HA/PLLA films showed lower surface contact angle and faster degradation degree compared with PLLA films.
27498308	10	96	theme	value	1619:1623	arg1	length					1625:1630	an average value length	1608:1630	an average value length of 122 ± 5 μm	1608:1644	ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 ± 5 μm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups.
27498308	2	97	theme	novel	372:376	arg1	HA					395:396	HA	395:396	HA	395:396	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27498308	2	97	theme	novel	372:376	arg1	acid					389:392	novel hyaluronic acid	372:392	novel hyaluronic acid (HA)-doped PEDOT nanoparticles	372:423	In this study, novel hyaluronic acid (HA)-doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(l-lactic acid) (PLLA) composite films were prepared.
27810279	3	0	theme	affinity	338:345	arg1	procedure					347:355	an affinity procedure	335:355	an affinity procedure based on a magnetic Dacron-HEP composite	335:396	In this study, an affinity procedure based on a magnetic Dacron-HEP composite is proposed.
27810279	0	1	theme	affinity	96:103	arg1	purification					105:116	antithrombin affinity purification	83:116	antithrombin affinity purification	83:116	Synthesis and characterisation of magnetised Dacron-heparin composite employed for antithrombin affinity purification.
27810279	3	2	theme	Dacron-HEP	377:386	arg1	composite					388:396	a magnetic Dacron-HEP composite	366:396	a magnetic Dacron-HEP composite	366:396	In this study, an affinity procedure based on a magnetic Dacron-HEP composite is proposed.
27810279	12	3	dep	easy	1298:1301	arg1	low-cost					1304:1311	low-cost	1304:1311	low-cost	1304:1311	This method presents the following advantages: easy, low-cost synthesis of the composite, magnet-based affinity purification steps, and reusability.
27810279	8	4	theme	NaCl	906:909	arg1	concentrations					888:901	increasing concentrations	877:901	increasing concentrations of NaCl	877:909	Human plasma was incubated with mDAC-HEP (fresh and stored over a long period) and washed with phosphate buffer containing increasing concentrations of NaCl.
27810279	10	5	theme	eluates	1131:1137	arg1	Electrophoresis					1108:1122	Electrophoresis	1108:1122	Electrophoresis of the eluates	1108:1137	Electrophoresis of the eluates revealed bands corresponding to the expected size of antithrombin (58kDa).
27810279	9	6	theme	coagulation	1059:1069	arg1	aPTT					1077:1080	aPTT	1077:1080	aPTT	1077:1080	Human plasma antithrombin activity was reduced by approximately 20% in the presence of the 1.0M NaCl fraction, and this eluate was able to prolong coagulation time (aPTT) using both preparations.
27810279	9	6	theme	coagulation	1059:1069	arg1	time					1071:1074	coagulation time	1059:1074	coagulation time (aPTT) using both preparations	1059:1105	Human plasma antithrombin activity was reduced by approximately 20% in the presence of the 1.0M NaCl fraction, and this eluate was able to prolong coagulation time (aPTT) using both preparations.
27810279	3	7	theme	magnetic	368:375	arg1	composite					388:396	a magnetic Dacron-HEP composite	366:396	a magnetic Dacron-HEP composite	366:396	In this study, an affinity procedure based on a magnetic Dacron-HEP composite is proposed.
27810279	2	8	theme	antithrombin	293:304	arg1	purification					306:317	antithrombin purification	293:317	antithrombin purification	293:317	Affinity chromatography using heparin (HEP) derivatives is usually used for antithrombin purification.
27810279	2	9	used	used	284:287	arg2	chromatography					226:239	Affinity chromatography	217:239	Affinity chromatography using heparin (HEP) derivatives	217:271	Affinity chromatography using heparin (HEP) derivatives is usually used for antithrombin purification.
27810279	1	10	theme	coagulation	192:202	arg1	dysfunction					204:214	coagulation dysfunction	192:214	coagulation dysfunction	192:214	Human antithrombin is a blood derivative widely used in the treatment of coagulation dysfunction.
27810279	6	11	theme	spectra	639:645	arg1	analyses					647:654	EDX and infrared spectra analyses	622:654	EDX and infrared spectra analyses	622:654	EDX and infrared spectra analyses confirmed each synthesis step of mDAC-HEP.
27810279	8	12	theme	Human	754:758	arg1	plasma					760:765	Human plasma	754:765	Human plasma	754:765	Human plasma was incubated with mDAC-HEP (fresh and stored over a long period) and washed with phosphate buffer containing increasing concentrations of NaCl.
27810279	12	13	theme	composite	1330:1338	arg1	steps					1376:1380	the composite, magnet-based affinity purification steps	1326:1380	the composite, magnet-based affinity purification steps	1326:1380	This method presents the following advantages: easy, low-cost synthesis of the composite, magnet-based affinity purification steps, and reusability.
27810279	5	14	attach	linked	595:600	arg2	HEP					521:523	HEP	521:523	HEP	521:523	HEP was activated by carbodiimide and N-hydroxysuccinimide and covalently linked to mDAC (mDAC-HEP).
27810279	5	14	attach	linked	595:600	arg1	mDAC-HEP					611:618	mDAC-HEP	611:618	mDAC-HEP	611:618	HEP was activated by carbodiimide and N-hydroxysuccinimide and covalently linked to mDAC (mDAC-HEP).
27810279	5	14	attach	linked	595:600	arg1	mDAC					605:608	mDAC	605:608	mDAC (mDAC-HEP)	605:619	HEP was activated by carbodiimide and N-hydroxysuccinimide and covalently linked to mDAC (mDAC-HEP).
27810279	1	15	theme	dysfunction	204:214	arg1	treatment					179:187	the treatment	175:187	the treatment of coagulation dysfunction	175:214	Human antithrombin is a blood derivative widely used in the treatment of coagulation dysfunction.
27810279	6	16	theme	infrared	630:637	arg1	spectra					639:645	infrared spectra	630:645	infrared spectra	630:645	EDX and infrared spectra analyses confirmed each synthesis step of mDAC-HEP.
27810279	1	17	theme	Human	119:123	arg1	derivative					149:158	a blood derivative	141:158	a blood derivative widely used in the treatment of coagulation dysfunction	141:214	Human antithrombin is a blood derivative widely used in the treatment of coagulation dysfunction.
27810279	1	17	theme	Human	119:123	arg1	antithrombin					125:136	Human antithrombin	119:136	Human antithrombin	119:136	Human antithrombin is a blood derivative widely used in the treatment of coagulation dysfunction.
27810279	4	18	with	co-precipitation	478:493	arg1	mDAC					514:517	mDAC	514:517	mDAC	514:517	Dacron was firstly converted to Dacron-hydrazide and magnetised by co-precipitation with of Fe2+/Fe3+ (mDAC).
27810279	4	18	with	co-precipitation	478:493	arg1	Fe2+/Fe3+					503:511	of Fe2+/Fe3+	500:511	of Fe2+/Fe3+ (mDAC)	500:518	Dacron was firstly converted to Dacron-hydrazide and magnetised by co-precipitation with of Fe2+/Fe3+ (mDAC).
27810279	9	19	theme	Human	912:916	arg1	activity					938:945	Human plasma antithrombin activity	912:945	Human plasma antithrombin activity	912:945	Human plasma antithrombin activity was reduced by approximately 20% in the presence of the 1.0M NaCl fraction, and this eluate was able to prolong coagulation time (aPTT) using both preparations.
27810279	12	20	theme	easy	1298:1301	arg1	synthesis					1313:1321	easy, low-cost synthesis	1298:1321	easy, low-cost synthesis of the composite, magnet-based affinity purification steps	1298:1380	This method presents the following advantages: easy, low-cost synthesis of the composite, magnet-based affinity purification steps, and reusability.
27810279	6	21	theme	EDX	622:624	arg1	analyses					647:654	EDX and infrared spectra analyses	622:654	EDX and infrared spectra analyses	622:654	EDX and infrared spectra analyses confirmed each synthesis step of mDAC-HEP.
27810279	9	22	theme	plasma	918:923	arg1	activity					938:945	Human plasma antithrombin activity	912:945	Human plasma antithrombin activity	912:945	Human plasma antithrombin activity was reduced by approximately 20% in the presence of the 1.0M NaCl fraction, and this eluate was able to prolong coagulation time (aPTT) using both preparations.
27810279	11	23	theme	mDAC-HEP	1218:1225	arg1	reusable					1241:1248	reusable	1241:1248	reusable	1241:1248	The mDAC-HEP particles are reusable.
27810279	11	23	theme	mDAC-HEP	1218:1225	arg1	particles					1227:1235	The mDAC-HEP particles	1214:1235	The mDAC-HEP particles	1214:1235	The mDAC-HEP particles are reusable.
27810279	0	24	theme	Dacron-heparin	45:58	arg1	composite					60:68	magnetised Dacron-heparin composite	34:68	magnetised Dacron-heparin composite	34:68	Synthesis and characterisation of magnetised Dacron-heparin composite employed for antithrombin affinity purification.
27810279	9	25	theme	antithrombin	925:936	arg1	activity					938:945	Human plasma antithrombin activity	912:945	Human plasma antithrombin activity	912:945	Human plasma antithrombin activity was reduced by approximately 20% in the presence of the 1.0M NaCl fraction, and this eluate was able to prolong coagulation time (aPTT) using both preparations.
27810279	0	26	theme	magnetised	34:43	arg1	composite					60:68	magnetised Dacron-heparin composite	34:68	magnetised Dacron-heparin composite	34:68	Synthesis and characterisation of magnetised Dacron-heparin composite employed for antithrombin affinity purification.
27810279	10	27	theme	antithrombin	1192:1203	arg1	size					1184:1187	the expected size	1171:1187	the expected size of antithrombin (58kDa)	1171:1211	Electrophoresis of the eluates revealed bands corresponding to the expected size of antithrombin (58kDa).
27810279	8	28	dep	mDAC-HEP	786:793	arg1	fresh					796:800	fresh	796:800	fresh	796:800	Human plasma was incubated with mDAC-HEP (fresh and stored over a long period) and washed with phosphate buffer containing increasing concentrations of NaCl.
27810279	8	28	dep	mDAC-HEP	786:793	arg1	stored					806:811	stored	806:811	stored	806:811	Human plasma was incubated with mDAC-HEP (fresh and stored over a long period) and washed with phosphate buffer containing increasing concentrations of NaCl.
27810279	12	29	theme	steps	1376:1380	arg1	reusability					1387:1397	reusability	1387:1397	reusability	1387:1397	This method presents the following advantages: easy, low-cost synthesis of the composite, magnet-based affinity purification steps, and reusability.
27810279	12	29	theme	steps	1376:1380	arg1	synthesis					1313:1321	easy, low-cost synthesis	1298:1321	easy, low-cost synthesis of the composite, magnet-based affinity purification steps	1298:1380	This method presents the following advantages: easy, low-cost synthesis of the composite, magnet-based affinity purification steps, and reusability.
27810279	12	29	theme	steps	1376:1380	arg1	advantages					1286:1295	the following advantages	1272:1295	the following advantages	1272:1295	This method presents the following advantages: easy, low-cost synthesis of the composite, magnet-based affinity purification steps, and reusability.
27810279	9	30	theme	1.0M	1003:1006	arg1	fraction					1013:1020	the 1.0M NaCl fraction	999:1020	the 1.0M NaCl fraction	999:1020	Human plasma antithrombin activity was reduced by approximately 20% in the presence of the 1.0M NaCl fraction, and this eluate was able to prolong coagulation time (aPTT) using both preparations.
27810279	4	31	theme	of	500:501	arg1	mDAC					514:517	mDAC	514:517	mDAC	514:517	Dacron was firstly converted to Dacron-hydrazide and magnetised by co-precipitation with of Fe2+/Fe3+ (mDAC).
27810279	4	31	theme	of	500:501	arg1	Fe2+/Fe3+					503:511	of Fe2+/Fe3+	500:511	of Fe2+/Fe3+ (mDAC)	500:518	Dacron was firstly converted to Dacron-hydrazide and magnetised by co-precipitation with of Fe2+/Fe3+ (mDAC).
27810279	2	32	theme	HEP	256:258	arg1	derivatives					261:271	heparin (HEP) derivatives	247:271	heparin (HEP) derivatives	247:271	Affinity chromatography using heparin (HEP) derivatives is usually used for antithrombin purification.
27810279	0	33	theme	composite	60:68	arg1	characterisation					14:29	characterisation	14:29	characterisation	14:29	Synthesis and characterisation of magnetised Dacron-heparin composite employed for antithrombin affinity purification.
27810279	0	33	theme	composite	60:68	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterisation of magnetised Dacron-heparin composite employed for antithrombin affinity purification.
27810279	2	34	theme	heparin	247:253	arg1	derivatives					261:271	heparin (HEP) derivatives	247:271	heparin (HEP) derivatives	247:271	Affinity chromatography using heparin (HEP) derivatives is usually used for antithrombin purification.
27810279	1	35	theme	blood	143:147	arg1	derivative					149:158	a blood derivative	141:158	a blood derivative widely used in the treatment of coagulation dysfunction	141:214	Human antithrombin is a blood derivative widely used in the treatment of coagulation dysfunction.
27810279	1	35	theme	blood	143:147	arg1	antithrombin					125:136	Human antithrombin	119:136	Human antithrombin	119:136	Human antithrombin is a blood derivative widely used in the treatment of coagulation dysfunction.
27810279	10	36	theme	expected	1175:1182	arg1	size					1184:1187	the expected size	1171:1187	the expected size of antithrombin (58kDa)	1171:1211	Electrophoresis of the eluates revealed bands corresponding to the expected size of antithrombin (58kDa).
27810279	7	37	theme	superparamagnetism	724:741	arg1	behaviour					743:751	superparamagnetism behaviour	724:751	superparamagnetism behaviour	724:751	This composite exhibited superparamagnetism behaviour.
27810279	12	38	dep	composite	1330:1338	arg1	magnet-based					1341:1352	magnet-based	1341:1352	magnet-based	1341:1352	This method presents the following advantages: easy, low-cost synthesis of the composite, magnet-based affinity purification steps, and reusability.
27810279	6	39	theme	mDAC-HEP	689:696	arg1	step					681:684	each synthesis step	666:684	each synthesis step of mDAC-HEP	666:696	EDX and infrared spectra analyses confirmed each synthesis step of mDAC-HEP.
27810279	12	40	theme	following	1276:1284	arg1	advantages					1286:1295	the following advantages	1272:1295	the following advantages	1272:1295	This method presents the following advantages: easy, low-cost synthesis of the composite, magnet-based affinity purification steps, and reusability.
27810279	8	41	theme	increasing	877:886	arg1	concentrations					888:901	increasing concentrations	877:901	increasing concentrations of NaCl	877:909	Human plasma was incubated with mDAC-HEP (fresh and stored over a long period) and washed with phosphate buffer containing increasing concentrations of NaCl.
27810279	0	42	theme	antithrombin	83:94	arg1	purification					105:116	antithrombin affinity purification	83:116	antithrombin affinity purification	83:116	Synthesis and characterisation of magnetised Dacron-heparin composite employed for antithrombin affinity purification.
27810279	2	43	theme	Affinity	217:224	arg1	chromatography					226:239	Affinity chromatography	217:239	Affinity chromatography using heparin (HEP) derivatives	217:271	Affinity chromatography using heparin (HEP) derivatives is usually used for antithrombin purification.
27810279	12	44	theme	purification	1363:1374	arg1	steps					1376:1380	the composite, magnet-based affinity purification steps	1326:1380	the composite, magnet-based affinity purification steps	1326:1380	This method presents the following advantages: easy, low-cost synthesis of the composite, magnet-based affinity purification steps, and reusability.
27810279	9	45	theme	fraction	1013:1020	arg1	presence					987:994	the presence	983:994	the presence of the 1.0M NaCl fraction	983:1020	Human plasma antithrombin activity was reduced by approximately 20% in the presence of the 1.0M NaCl fraction, and this eluate was able to prolong coagulation time (aPTT) using both preparations.
27810279	8	46	theme	phosphate	849:857	arg1	buffer					859:864	phosphate buffer	849:864	phosphate buffer containing increasing concentrations of NaCl	849:909	Human plasma was incubated with mDAC-HEP (fresh and stored over a long period) and washed with phosphate buffer containing increasing concentrations of NaCl.
27810279	8	47	contain	containing	866:875	arg1	buffer					859:864	phosphate buffer	849:864	phosphate buffer containing increasing concentrations of NaCl	849:909	Human plasma was incubated with mDAC-HEP (fresh and stored over a long period) and washed with phosphate buffer containing increasing concentrations of NaCl.
27810279	8	47	contain	containing	866:875	arg2	concentrations					888:901	increasing concentrations	877:901	increasing concentrations of NaCl	877:909	Human plasma was incubated with mDAC-HEP (fresh and stored over a long period) and washed with phosphate buffer containing increasing concentrations of NaCl.
27810279	12	48	theme	affinity	1354:1361	arg1	steps					1376:1380	the composite, magnet-based affinity purification steps	1326:1380	the composite, magnet-based affinity purification steps	1326:1380	This method presents the following advantages: easy, low-cost synthesis of the composite, magnet-based affinity purification steps, and reusability.
27810279	9	49	theme	NaCl	1008:1011	arg1	fraction					1013:1020	the 1.0M NaCl fraction	999:1020	the 1.0M NaCl fraction	999:1020	Human plasma antithrombin activity was reduced by approximately 20% in the presence of the 1.0M NaCl fraction, and this eluate was able to prolong coagulation time (aPTT) using both preparations.
27810279	6	50	theme	synthesis	671:679	arg1	step					681:684	each synthesis step	666:684	each synthesis step of mDAC-HEP	666:696	EDX and infrared spectra analyses confirmed each synthesis step of mDAC-HEP.
27810279	8	51	theme	long	820:823	arg1	period					825:830	a long period	818:830	a long period	818:830	Human plasma was incubated with mDAC-HEP (fresh and stored over a long period) and washed with phosphate buffer containing increasing concentrations of NaCl.
25453286	6	0	theme	10B	812:814	arg1	kinetics					827:834	Amido Black 10B adsorption kinetics	800:834	Amido Black 10B adsorption kinetics	800:834	Amido Black 10B adsorption kinetics followed a pseudo-second-order kinetic model.
25453286	7	1	theme	thermodynamic	897:909	arg1	parameters					911:920	The calculated thermodynamic parameters	882:920	The calculated thermodynamic parameters	882:920	The calculated thermodynamic parameters showed that the adsorption of Amido Black 10B by CCS/BT composite was spontaneous and endothermic in nature.
25453286	1	2	theme	cross-linking	214:226	arg1	reaction					228:235	the cross-linking reaction	210:235	the cross-linking reaction between chitosan and glutaraldehyde	210:271	In this study, cross-linked chitosan (CCS)/bentonite (BT) composite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde.
25453286	6	3	theme	Black	806:810	arg1	kinetics					827:834	Amido Black 10B adsorption kinetics	800:834	Amido Black 10B adsorption kinetics	800:834	Amido Black 10B adsorption kinetics followed a pseudo-second-order kinetic model.
25453286	6	4	theme	Amido	800:804	arg1	kinetics					827:834	Amido Black 10B adsorption kinetics	800:834	Amido Black 10B adsorption kinetics	800:834	Amido Black 10B adsorption kinetics followed a pseudo-second-order kinetic model.
25453286	2	5	theme	CCS/BT	274:279	arg1	composite					281:289	CCS/BT composite	274:289	CCS/BT composite	274:289	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	2	6	dep	transform	326:334	arg1	infrared					336:343	infrared	336:343	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA)	326:463	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	7	7	from	spontaneous	992:1002	arg1	nature					1023:1028	nature	1023:1028	nature	1023:1028	The calculated thermodynamic parameters showed that the adsorption of Amido Black 10B by CCS/BT composite was spontaneous and endothermic in nature.
25453286	5	8	theme	adsorption	747:756	arg1	capacity					758:765	the maximum adsorption capacity	735:765	the maximum adsorption capacity	735:765	The adsorption isotherm was well described by the Langmuir model and the maximum adsorption capacity was 323.6 mg/g at 293K and pH 2.
25453286	5	8	theme	adsorption	747:756	arg1	mg/g					777:780	323.6 mg/g	771:780	323.6 mg/g	771:780	The adsorption isotherm was well described by the Langmuir model and the maximum adsorption capacity was 323.6 mg/g at 293K and pH 2.
25453286	1	9	theme	composite	137:145	arg1	CCS					117:119	CCS	117:119	CCS	117:119	In this study, cross-linked chitosan (CCS)/bentonite (BT) composite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde.
25453286	1	9	theme	composite	137:145	arg1	chitosan					107:114	cross-linked chitosan	94:114	cross-linked chitosan (CCS)/bentonite (BT) composite	94:145	In this study, cross-linked chitosan (CCS)/bentonite (BT) composite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde.
25453286	0	10	theme	dye	29:31	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of an anionic azo dye by cross-linked chitosan/bentonite composite.	0:77	Adsorption of an anionic azo dye by cross-linked chitosan/bentonite composite.
25453286	0	11	link	cross-linked	36:47	arg1	composite					68:76	cross-linked chitosan/bentonite composite	36:76	cross-linked chitosan/bentonite composite	36:76	Adsorption of an anionic azo dye by cross-linked chitosan/bentonite composite.
25453286	2	12	theme	electron	375:382	arg1	SEM					396:398	SEM	396:398	SEM	396:398	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	2	12	theme	electron	375:382	arg1	microscopy					384:393	scanning electron microscopy	366:393	scanning electron microscopy (SEM)	366:399	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	5	13	theme	Langmuir	716:723	arg1	model					725:729	the Langmuir model	712:729	the Langmuir model	712:729	The adsorption isotherm was well described by the Langmuir model and the maximum adsorption capacity was 323.6 mg/g at 293K and pH 2.
25453286	7	14	from	nature	1023:1028	arg1	adsorption					938:947	the adsorption	934:947	the adsorption of Amido Black 10B by CCS/BT composite	934:986	The calculated thermodynamic parameters showed that the adsorption of Amido Black 10B by CCS/BT composite was spontaneous and endothermic in nature.
25453286	7	14	from	nature	1023:1028	arg1	spontaneous					992:1002	spontaneous	992:1002	spontaneous	992:1002	The calculated thermodynamic parameters showed that the adsorption of Amido Black 10B by CCS/BT composite was spontaneous and endothermic in nature.
25453286	2	15	dep	Fourier	318:324	arg1	transform					326:334	transform	326:334	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA)	326:463	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	2	16	theme	scanning	366:373	arg1	SEM					396:398	SEM	396:398	SEM	396:398	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	2	16	theme	scanning	366:373	arg1	microscopy					384:393	scanning electron microscopy	366:393	scanning electron microscopy (SEM)	366:399	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	3	17	theme	azo	525:527	arg1	10B					546:548	Amido Black 10B	534:548	Amido Black 10B	534:548	Their adsorption characteristics were assessed by using an azo dye (Amido Black 10B) as a model adsorbate.
25453286	3	17	theme	azo	525:527	arg1	dye					529:531	an azo dye	522:531	an azo dye (Amido Black 10B)	522:549	Their adsorption characteristics were assessed by using an azo dye (Amido Black 10B) as a model adsorbate.
25453286	3	17	theme	azo	525:527	arg1	adsorbate					562:570	a model adsorbate	554:570	a model adsorbate	554:570	Their adsorption characteristics were assessed by using an azo dye (Amido Black 10B) as a model adsorbate.
25453286	4	18	theme	Black	597:601	arg1	10B					603:605	Amido Black 10B	591:605	Amido Black 10B	591:605	The adsorption of Amido Black 10B onto the CCS/BT composite was found to be optimal at pH 2.
25453286	4	19	theme	10B	603:605	arg1	adsorption					577:586	The adsorption	573:586	The adsorption of Amido Black 10B onto the CCS/BT composite	573:631	The adsorption of Amido Black 10B onto the CCS/BT composite was found to be optimal at pH 2.
25453286	4	19	theme	10B	603:605	arg1	optimal					649:655	optimal	649:655	optimal	649:655	The adsorption of Amido Black 10B onto the CCS/BT composite was found to be optimal at pH 2.
25453286	0	20	theme	anionic	17:23	arg1	dye					29:31	an anionic azo dye	14:31	an anionic azo dye	14:31	Adsorption of an anionic azo dye by cross-linked chitosan/bentonite composite.
25453286	4	21	from	pH	660:661	arg1	adsorption					577:586	The adsorption	573:586	The adsorption of Amido Black 10B onto the CCS/BT composite	573:631	The adsorption of Amido Black 10B onto the CCS/BT composite was found to be optimal at pH 2.
25453286	4	21	from	pH	660:661	arg1	optimal					649:655	optimal	649:655	optimal	649:655	The adsorption of Amido Black 10B onto the CCS/BT composite was found to be optimal at pH 2.
25453286	5	22	from	pH	794:795	arg1	capacity					758:765	the maximum adsorption capacity	735:765	the maximum adsorption capacity	735:765	The adsorption isotherm was well described by the Langmuir model and the maximum adsorption capacity was 323.6 mg/g at 293K and pH 2.
25453286	5	22	from	pH	794:795	arg1	mg/g					777:780	323.6 mg/g	771:780	323.6 mg/g	771:780	The adsorption isotherm was well described by the Langmuir model and the maximum adsorption capacity was 323.6 mg/g at 293K and pH 2.
25453286	5	23	theme	maximum	739:745	arg1	capacity					758:765	the maximum adsorption capacity	735:765	the maximum adsorption capacity	735:765	The adsorption isotherm was well described by the Langmuir model and the maximum adsorption capacity was 323.6 mg/g at 293K and pH 2.
25453286	5	23	theme	maximum	739:745	arg1	mg/g					777:780	323.6 mg/g	771:780	323.6 mg/g	771:780	The adsorption isotherm was well described by the Langmuir model and the maximum adsorption capacity was 323.6 mg/g at 293K and pH 2.
25453286	2	24	theme	X-ray	402:406	arg1	XRD					421:423	XRD	421:423	XRD	421:423	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	2	24	theme	X-ray	402:406	arg1	diffraction					408:418	X-ray diffraction	402:418	X-ray diffraction (XRD)	402:424	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	1	25	theme	cross-linked	94:105	arg1	CCS					117:119	CCS	117:119	CCS	117:119	In this study, cross-linked chitosan (CCS)/bentonite (BT) composite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde.
25453286	1	25	theme	cross-linked	94:105	arg1	chitosan					107:114	cross-linked chitosan	94:114	cross-linked chitosan (CCS)/bentonite (BT) composite	94:145	In this study, cross-linked chitosan (CCS)/bentonite (BT) composite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde.
25453286	4	26	theme	CCS/BT	616:621	arg1	composite					623:631	the CCS/BT composite	612:631	the CCS/BT composite	612:631	The adsorption of Amido Black 10B onto the CCS/BT composite was found to be optimal at pH 2.
25453286	2	27	theme	gravimetric	438:448	arg1	TGA					460:462	TGA	460:462	TGA	460:462	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	2	27	theme	gravimetric	438:448	arg1	analyses					450:457	thermal gravimetric analyses	430:457	thermal gravimetric analyses (TGA)	430:463	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	6	28	theme	kinetic	867:873	arg1	model					875:879	a pseudo-second-order kinetic model	845:879	a pseudo-second-order kinetic model	845:879	Amido Black 10B adsorption kinetics followed a pseudo-second-order kinetic model.
25453286	7	29	theme	CCS/BT	971:976	arg1	composite					978:986	CCS/BT composite	971:986	CCS/BT composite	971:986	The calculated thermodynamic parameters showed that the adsorption of Amido Black 10B by CCS/BT composite was spontaneous and endothermic in nature.
25453286	0	30	theme	azo	25:27	arg1	dye					29:31	an anionic azo dye	14:31	an anionic azo dye	14:31	Adsorption of an anionic azo dye by cross-linked chitosan/bentonite composite.
25453286	6	31	theme	pseudo-second-order	847:865	arg1	model					875:879	a pseudo-second-order kinetic model	845:879	a pseudo-second-order kinetic model	845:879	Amido Black 10B adsorption kinetics followed a pseudo-second-order kinetic model.
25453286	2	32	theme	thermal	430:436	arg1	TGA					460:462	TGA	460:462	TGA	460:462	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	2	32	theme	thermal	430:436	arg1	analyses					450:457	thermal gravimetric analyses	430:457	thermal gravimetric analyses (TGA)	430:463	CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA).
25453286	1	33	theme	chitosan	184:191	arg1	intercalation					167:179	the intercalation	163:179	the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde	163:271	In this study, cross-linked chitosan (CCS)/bentonite (BT) composite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde.
25453286	0	34	theme	cross-linked	36:47	arg1	composite					68:76	cross-linked chitosan/bentonite composite	36:76	cross-linked chitosan/bentonite composite	36:76	Adsorption of an anionic azo dye by cross-linked chitosan/bentonite composite.
25453286	7	35	theme	10B	964:966	arg1	adsorption					938:947	the adsorption	934:947	the adsorption of Amido Black 10B by CCS/BT composite	934:986	The calculated thermodynamic parameters showed that the adsorption of Amido Black 10B by CCS/BT composite was spontaneous and endothermic in nature.
25453286	7	35	theme	10B	964:966	arg1	spontaneous					992:1002	spontaneous	992:1002	spontaneous	992:1002	The calculated thermodynamic parameters showed that the adsorption of Amido Black 10B by CCS/BT composite was spontaneous and endothermic in nature.
25453286	0	36	theme	chitosan/bentonite	49:66	arg1	composite					68:76	cross-linked chitosan/bentonite composite	36:76	cross-linked chitosan/bentonite composite	36:76	Adsorption of an anionic azo dye by cross-linked chitosan/bentonite composite.
25453286	3	37	theme	Amido	534:538	arg1	10B					546:548	Amido Black 10B	534:548	Amido Black 10B	534:548	Their adsorption characteristics were assessed by using an azo dye (Amido Black 10B) as a model adsorbate.
25453286	3	37	theme	Amido	534:538	arg1	dye					529:531	an azo dye	522:531	an azo dye (Amido Black 10B)	522:549	Their adsorption characteristics were assessed by using an azo dye (Amido Black 10B) as a model adsorbate.
25453286	3	38	theme	Black	540:544	arg1	10B					546:548	Amido Black 10B	534:548	Amido Black 10B	534:548	Their adsorption characteristics were assessed by using an azo dye (Amido Black 10B) as a model adsorbate.
25453286	3	38	theme	Black	540:544	arg1	dye					529:531	an azo dye	522:531	an azo dye (Amido Black 10B)	522:549	Their adsorption characteristics were assessed by using an azo dye (Amido Black 10B) as a model adsorbate.
25453286	7	39	theme	Amido	952:956	arg1	10B					964:966	Amido Black 10B	952:966	Amido Black 10B	952:966	The calculated thermodynamic parameters showed that the adsorption of Amido Black 10B by CCS/BT composite was spontaneous and endothermic in nature.
25453286	3	40	theme	model	556:560	arg1	dye					529:531	an azo dye	522:531	an azo dye (Amido Black 10B)	522:549	Their adsorption characteristics were assessed by using an azo dye (Amido Black 10B) as a model adsorbate.
25453286	3	40	theme	model	556:560	arg1	adsorbate					562:570	a model adsorbate	554:570	a model adsorbate	554:570	Their adsorption characteristics were assessed by using an azo dye (Amido Black 10B) as a model adsorbate.
25453286	1	41	from	intercalation	167:179	arg1	reaction					228:235	the cross-linking reaction	210:235	the cross-linking reaction between chitosan and glutaraldehyde	210:271	In this study, cross-linked chitosan (CCS)/bentonite (BT) composite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde.
25453286	1	41	from	intercalation	167:179	arg1	bentonite					196:204	bentonite	196:204	bentonite	196:204	In this study, cross-linked chitosan (CCS)/bentonite (BT) composite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde.
25453286	7	42	theme	Black	958:962	arg1	10B					964:966	Amido Black 10B	952:966	Amido Black 10B	952:966	The calculated thermodynamic parameters showed that the adsorption of Amido Black 10B by CCS/BT composite was spontaneous and endothermic in nature.
25453286	5	43	theme	adsorption	670:679	arg1	isotherm					681:688	The adsorption isotherm	666:688	The adsorption isotherm	666:688	The adsorption isotherm was well described by the Langmuir model and the maximum adsorption capacity was 323.6 mg/g at 293K and pH 2.
25453286	5	44	from	293K	785:788	arg1	capacity					758:765	the maximum adsorption capacity	735:765	the maximum adsorption capacity	735:765	The adsorption isotherm was well described by the Langmuir model and the maximum adsorption capacity was 323.6 mg/g at 293K and pH 2.
25453286	5	44	from	293K	785:788	arg1	mg/g					777:780	323.6 mg/g	771:780	323.6 mg/g	771:780	The adsorption isotherm was well described by the Langmuir model and the maximum adsorption capacity was 323.6 mg/g at 293K and pH 2.
25453286	3	45	theme	adsorption	472:481	arg1	characteristics					483:497	Their adsorption characteristics	466:497	Their adsorption characteristics	466:497	Their adsorption characteristics were assessed by using an azo dye (Amido Black 10B) as a model adsorbate.
25453286	4	46	theme	Amido	591:595	arg1	10B					603:605	Amido Black 10B	591:605	Amido Black 10B	591:605	The adsorption of Amido Black 10B onto the CCS/BT composite was found to be optimal at pH 2.
25453286	1	47	link	cross-linked	94:105	arg1	CCS					117:119	CCS	117:119	CCS	117:119	In this study, cross-linked chitosan (CCS)/bentonite (BT) composite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde.
25453286	1	47	link	cross-linked	94:105	arg1	chitosan					107:114	cross-linked chitosan	94:114	cross-linked chitosan (CCS)/bentonite (BT) composite	94:145	In this study, cross-linked chitosan (CCS)/bentonite (BT) composite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde.
25453286	6	48	theme	adsorption	816:825	arg1	kinetics					827:834	Amido Black 10B adsorption kinetics	800:834	Amido Black 10B adsorption kinetics	800:834	Amido Black 10B adsorption kinetics followed a pseudo-second-order kinetic model.
25453286	7	49	theme	calculated	886:895	arg1	parameters					911:920	The calculated thermodynamic parameters	882:920	The calculated thermodynamic parameters	882:920	The calculated thermodynamic parameters showed that the adsorption of Amido Black 10B by CCS/BT composite was spontaneous and endothermic in nature.
26485926	7	0	theme	excellent	1004:1012	arg1	activity					1028:1035	excellent antibacterial activity	1004:1035	excellent antibacterial activity	1004:1035	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	1	1	theme	ideal	163:167	arg1	candidates					169:178	faujasites ideal candidates	152:178	faujasites ideal candidates for tissue engineering applications	152:214	The biocompatibility and excellent ion exchange capacity make faujasites ideal candidates for tissue engineering applications.
26485926	0	2	theme	Composite	69:77	arg1	Membranes					79:87	Faujasite Composite Membranes	59:87	Faujasite Composite Membranes	59:87	In Vitro and In Vivo Evaluation of Pectin/Copper Exchanged Faujasite Composite Membranes.
26485926	4	3	theme	elemental	551:559	arg1	composition					561:571	their elemental composition	545:571	their elemental composition	545:571	The morphology of composite membranes was characterized by SEM and their elemental composition was determined using EDX.
26485926	5	4	theme	native	659:664	arg1	pectin					666:671	native pectin	659:671	native pectin	659:671	The higher contact angle of P (1%) when compared to that of native pectin figured out an enhanced hydrophobicity of hybrid material.
26485926	8	5	theme	fibroblast	1129:1138	arg1	lines					1145:1149	NIH3T3 fibroblast cell lines	1122:1149	NIH3T3 fibroblast cell lines	1122:1149	Finally, it displayed cell viability of 89% on NIH3T3 fibroblast cell lines and aided in improving wound healing and re-epithelialisation in Sprague Dawley rats.
26485926	3	6	theme	calcium	425:431	arg1	chains					453:458	calcium cross-linked pectin chains	425:458	calcium cross-linked pectin chains used as a matrix	425:475	AFM images revealed the egg-box model organization of calcium cross-linked pectin chains used as a matrix.
26485926	7	7	theme	composite	897:905	arg1	membrane					907:914	The composite membrane	893:914	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%),	893:966	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	3	8	theme	cross-linked	433:444	arg1	chains					453:458	calcium cross-linked pectin chains	425:458	calcium cross-linked pectin chains used as a matrix	425:475	AFM images revealed the egg-box model organization of calcium cross-linked pectin chains used as a matrix.
26485926	3	9	theme	egg-box	395:401	arg1	organization					409:420	the egg-box model organization	391:420	the egg-box model organization of calcium cross-linked pectin chains used as a matrix	391:475	AFM images revealed the egg-box model organization of calcium cross-linked pectin chains used as a matrix.
26485926	9	10	theme	ideal	1284:1288	arg1	matrices					1290:1297	ideal matrices	1284:1297	ideal matrices	1284:1297	The obtained data suggested their potential as ideal matrices for efficient treatment of burn wounds.
26485926	7	11	theme	faujasite	950:958	arg1	%					922:922	1%	921:922	1% (w/w) of copper exchanged faujasite, P(1%),	921:966	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	7	11	theme	faujasite	950:958	arg1	P					961:961	P	961:961	P(1%)	961:965	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	7	11	theme	faujasite	950:958	arg1	faujasite					950:958	copper exchanged faujasite	933:958	copper exchanged faujasite	933:958	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	7	11	theme	faujasite	950:958	arg1	w/w					925:927	w/w	925:927	w/w	925:927	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	4	12	theme	membranes	506:514	arg1	morphology					482:491	The morphology	478:491	The morphology of composite membranes	478:514	The morphology of composite membranes was characterized by SEM and their elemental composition was determined using EDX.
26485926	7	13	with	membrane	907:914	arg1	%					922:922	1%	921:922	1% (w/w) of copper exchanged faujasite, P(1%),	921:966	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	7	13	with	membrane	907:914	arg1	P					961:961	P	961:961	P(1%)	961:965	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	7	13	with	membrane	907:914	arg1	faujasite					950:958	copper exchanged faujasite	933:958	copper exchanged faujasite	933:958	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	7	13	with	membrane	907:914	arg1	w/w					925:927	w/w	925:927	w/w	925:927	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	8	14	theme	cell	1140:1143	arg1	lines					1145:1149	NIH3T3 fibroblast cell lines	1122:1149	NIH3T3 fibroblast cell lines	1122:1149	Finally, it displayed cell viability of 89% on NIH3T3 fibroblast cell lines and aided in improving wound healing and re-epithelialisation in Sprague Dawley rats.
26485926	5	15	theme	contact	610:616	arg1	angle					618:622	The higher contact angle	599:622	The higher contact angle of P (1%)	599:632	The higher contact angle of P (1%) when compared to that of native pectin figured out an enhanced hydrophobicity of hybrid material.
26485926	2	16	theme	casting	302:308	arg1	technique					310:318	solvent casting technique	294:318	solvent casting technique	294:318	A novel pectin/copper exchanged faujasite hybrid membrane was synthesized by solvent casting technique, using calcium chloride as the crosslinking agent.
26485926	1	17	theme	tissue	184:189	arg1	applications					203:214	tissue engineering applications	184:214	tissue engineering applications	184:214	The biocompatibility and excellent ion exchange capacity make faujasites ideal candidates for tissue engineering applications.
26485926	3	18	theme	model	403:407	arg1	organization					409:420	the egg-box model organization	391:420	the egg-box model organization of calcium cross-linked pectin chains used as a matrix	391:475	AFM images revealed the egg-box model organization of calcium cross-linked pectin chains used as a matrix.
26485926	7	19	theme	exchanged	940:948	arg1	P					961:961	P	961:961	P(1%)	961:965	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	7	19	theme	exchanged	940:948	arg1	faujasite					950:958	copper exchanged faujasite	933:958	copper exchanged faujasite	933:958	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	1	20	theme	engineering	191:201	arg1	applications					203:214	tissue engineering applications	184:214	tissue engineering applications	184:214	The biocompatibility and excellent ion exchange capacity make faujasites ideal candidates for tissue engineering applications.
26485926	0	21	theme	In	0:1	arg1	Evaluation					21:30	In Vitro and In Vivo Evaluation	0:30	In Vitro and In Vivo Evaluation of Pectin/Copper	0:47	In Vitro and In Vivo Evaluation of Pectin/Copper Exchanged Faujasite Composite Membranes.
26485926	9	22	theme	obtained	1241:1248	arg1	data					1250:1253	The obtained data	1237:1253	The obtained data	1237:1253	The obtained data suggested their potential as ideal matrices for efficient treatment of burn wounds.
26485926	8	23	theme	Dawley	1224:1229	arg1	rats					1231:1234	Sprague Dawley rats	1216:1234	Sprague Dawley rats	1216:1234	Finally, it displayed cell viability of 89% on NIH3T3 fibroblast cell lines and aided in improving wound healing and re-epithelialisation in Sprague Dawley rats.
26485926	0	24	theme	In	13:14	arg1	Evaluation					21:30	In Vitro and In Vivo Evaluation	0:30	In Vitro and In Vivo Evaluation of Pectin/Copper	0:47	In Vitro and In Vivo Evaluation of Pectin/Copper Exchanged Faujasite Composite Membranes.
26485926	2	25	theme	exchanged	239:247	arg1	membrane					266:273	A novel pectin/copper exchanged faujasite hybrid membrane	217:273	A novel pectin/copper exchanged faujasite hybrid membrane	217:273	A novel pectin/copper exchanged faujasite hybrid membrane was synthesized by solvent casting technique, using calcium chloride as the crosslinking agent.
26485926	6	26	with	interactions	833:844	arg1	matrix					863:868	the polymer matrix	851:868	the polymer matrix	851:868	The embedded faujasite particles maintained their crystalline structure as revealed by XRD and their interactions with the polymer matrix was evaluated by FTIR.
26485926	9	27	theme	burn	1326:1329	arg1	wounds					1331:1336	burn wounds	1326:1336	burn wounds	1326:1336	The obtained data suggested their potential as ideal matrices for efficient treatment of burn wounds.
26485926	5	28	theme	hybrid	715:720	arg1	material					722:729	hybrid material	715:729	hybrid material	715:729	The higher contact angle of P (1%) when compared to that of native pectin figured out an enhanced hydrophobicity of hybrid material.
26485926	2	29	theme	calcium	327:333	arg1	chloride					335:342	calcium chloride	327:342	calcium chloride	327:342	A novel pectin/copper exchanged faujasite hybrid membrane was synthesized by solvent casting technique, using calcium chloride as the crosslinking agent.
26485926	2	29	theme	calcium	327:333	arg1	agent					364:368	the crosslinking agent	347:368	the crosslinking agent	347:368	A novel pectin/copper exchanged faujasite hybrid membrane was synthesized by solvent casting technique, using calcium chloride as the crosslinking agent.
26485926	8	30	theme	%	1117:1117	arg1	viability					1102:1110	cell viability	1097:1110	cell viability of 89%	1097:1117	Finally, it displayed cell viability of 89% on NIH3T3 fibroblast cell lines and aided in improving wound healing and re-epithelialisation in Sprague Dawley rats.
26485926	7	31	theme	copper	933:938	arg1	P					961:961	P	961:961	P(1%)	961:965	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	7	31	theme	copper	933:938	arg1	faujasite					950:958	copper exchanged faujasite	933:958	copper exchanged faujasite	933:958	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	3	32	theme	chains	453:458	arg1	organization					409:420	the egg-box model organization	391:420	the egg-box model organization of calcium cross-linked pectin chains used as a matrix	391:475	AFM images revealed the egg-box model organization of calcium cross-linked pectin chains used as a matrix.
26485926	9	33	theme	wounds	1331:1336	arg1	treatment					1313:1321	efficient treatment	1303:1321	efficient treatment of burn wounds	1303:1336	The obtained data suggested their potential as ideal matrices for efficient treatment of burn wounds.
26485926	5	34	theme	material	722:729	arg1	hydrophobicity					697:710	an enhanced hydrophobicity	685:710	an enhanced hydrophobicity of hybrid material	685:729	The higher contact angle of P (1%) when compared to that of native pectin figured out an enhanced hydrophobicity of hybrid material.
26485926	3	35	link	cross-linked	433:444	arg1	chains					453:458	calcium cross-linked pectin chains	425:458	calcium cross-linked pectin chains used as a matrix	425:475	AFM images revealed the egg-box model organization of calcium cross-linked pectin chains used as a matrix.
26485926	8	36	theme	wound	1174:1178	arg1	healing					1180:1186	wound healing	1174:1186	wound healing	1174:1186	Finally, it displayed cell viability of 89% on NIH3T3 fibroblast cell lines and aided in improving wound healing and re-epithelialisation in Sprague Dawley rats.
26485926	7	37	theme	controlled	1038:1047	arg1	swelling					1049:1056	controlled swelling	1038:1056	controlled swelling	1038:1056	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	4	38	theme	composite	496:504	arg1	membranes					506:514	composite membranes	496:514	composite membranes	496:514	The morphology of composite membranes was characterized by SEM and their elemental composition was determined using EDX.
26485926	2	39	theme	hybrid	259:264	arg1	membrane					266:273	A novel pectin/copper exchanged faujasite hybrid membrane	217:273	A novel pectin/copper exchanged faujasite hybrid membrane	217:273	A novel pectin/copper exchanged faujasite hybrid membrane was synthesized by solvent casting technique, using calcium chloride as the crosslinking agent.
26485926	7	40	theme	thermal	985:991	arg1	stability					993:1001	better thermal stability	978:1001	better thermal stability	978:1001	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	0	41	theme	Pectin/Copper	35:47	arg1	Evaluation					21:30	In Vitro and In Vivo Evaluation	0:30	In Vitro and In Vivo Evaluation of Pectin/Copper	0:47	In Vitro and In Vivo Evaluation of Pectin/Copper Exchanged Faujasite Composite Membranes.
26485926	7	42	theme	antibacterial	1014:1026	arg1	activity					1028:1035	excellent antibacterial activity	1004:1035	excellent antibacterial activity	1004:1035	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	2	43	theme	faujasite	249:257	arg1	membrane					266:273	A novel pectin/copper exchanged faujasite hybrid membrane	217:273	A novel pectin/copper exchanged faujasite hybrid membrane	217:273	A novel pectin/copper exchanged faujasite hybrid membrane was synthesized by solvent casting technique, using calcium chloride as the crosslinking agent.
26485926	6	44	theme	crystalline	782:792	arg1	structure					794:802	their crystalline structure	776:802	their crystalline structure	776:802	The embedded faujasite particles maintained their crystalline structure as revealed by XRD and their interactions with the polymer matrix was evaluated by FTIR.
26485926	1	45	theme	excellent	115:123	arg1	capacity					138:145	excellent ion exchange capacity	115:145	excellent ion exchange capacity	115:145	The biocompatibility and excellent ion exchange capacity make faujasites ideal candidates for tissue engineering applications.
26485926	3	46	theme	pectin	446:451	arg1	chains					453:458	calcium cross-linked pectin chains	425:458	calcium cross-linked pectin chains used as a matrix	425:475	AFM images revealed the egg-box model organization of calcium cross-linked pectin chains used as a matrix.
26485926	2	47	theme	pectin/copper	225:237	arg1	membrane					266:273	A novel pectin/copper exchanged faujasite hybrid membrane	217:273	A novel pectin/copper exchanged faujasite hybrid membrane	217:273	A novel pectin/copper exchanged faujasite hybrid membrane was synthesized by solvent casting technique, using calcium chloride as the crosslinking agent.
26485926	2	48	theme	novel	219:223	arg1	membrane					266:273	A novel pectin/copper exchanged faujasite hybrid membrane	217:273	A novel pectin/copper exchanged faujasite hybrid membrane	217:273	A novel pectin/copper exchanged faujasite hybrid membrane was synthesized by solvent casting technique, using calcium chloride as the crosslinking agent.
26485926	1	49	theme	ion	125:127	arg1	capacity					138:145	excellent ion exchange capacity	115:145	excellent ion exchange capacity	115:145	The biocompatibility and excellent ion exchange capacity make faujasites ideal candidates for tissue engineering applications.
26485926	5	50	theme	P	627:627	arg1	angle					618:622	The higher contact angle	599:622	The higher contact angle of P (1%)	599:632	The higher contact angle of P (1%) when compared to that of native pectin figured out an enhanced hydrophobicity of hybrid material.
26485926	5	51	theme	enhanced	688:695	arg1	hydrophobicity					697:710	an enhanced hydrophobicity	685:710	an enhanced hydrophobicity of hybrid material	685:729	The higher contact angle of P (1%) when compared to that of native pectin figured out an enhanced hydrophobicity of hybrid material.
26485926	0	52	dep	In	13:14	arg1	Vivo					16:19	Vivo	16:19	Vivo	16:19	In Vitro and In Vivo Evaluation of Pectin/Copper Exchanged Faujasite Composite Membranes.
26485926	2	53	theme	solvent	294:300	arg1	technique					310:318	solvent casting technique	294:318	solvent casting technique	294:318	A novel pectin/copper exchanged faujasite hybrid membrane was synthesized by solvent casting technique, using calcium chloride as the crosslinking agent.
26485926	5	54	theme	higher	603:608	arg1	angle					618:622	The higher contact angle	599:622	The higher contact angle of P (1%)	599:632	The higher contact angle of P (1%) when compared to that of native pectin figured out an enhanced hydrophobicity of hybrid material.
26485926	1	55	theme	exchange	129:136	arg1	capacity					138:145	excellent ion exchange capacity	115:145	excellent ion exchange capacity	115:145	The biocompatibility and excellent ion exchange capacity make faujasites ideal candidates for tissue engineering applications.
26485926	7	56	theme	better	978:983	arg1	stability					993:1001	better thermal stability	978:1001	better thermal stability	978:1001	The composite membrane with 1% (w/w) of copper exchanged faujasite, P(1%), exhibited better thermal stability, excellent antibacterial activity, controlled swelling and degradation.
26485926	6	57	theme	polymer	855:861	arg1	matrix					863:868	the polymer matrix	851:868	the polymer matrix	851:868	The embedded faujasite particles maintained their crystalline structure as revealed by XRD and their interactions with the polymer matrix was evaluated by FTIR.
26485926	2	58	theme	crosslinking	351:362	arg1	chloride					335:342	calcium chloride	327:342	calcium chloride	327:342	A novel pectin/copper exchanged faujasite hybrid membrane was synthesized by solvent casting technique, using calcium chloride as the crosslinking agent.
26485926	2	58	theme	crosslinking	351:362	arg1	agent					364:368	the crosslinking agent	347:368	the crosslinking agent	347:368	A novel pectin/copper exchanged faujasite hybrid membrane was synthesized by solvent casting technique, using calcium chloride as the crosslinking agent.
26485926	0	59	theme	Faujasite	59:67	arg1	Membranes					79:87	Faujasite Composite Membranes	59:87	Faujasite Composite Membranes	59:87	In Vitro and In Vivo Evaluation of Pectin/Copper Exchanged Faujasite Composite Membranes.
26485926	9	60	theme	efficient	1303:1311	arg1	treatment					1313:1321	efficient treatment	1303:1321	efficient treatment of burn wounds	1303:1336	The obtained data suggested their potential as ideal matrices for efficient treatment of burn wounds.
26485926	6	61	theme	faujasite	745:753	arg1	particles					755:763	The embedded faujasite particles	732:763	The embedded faujasite particles	732:763	The embedded faujasite particles maintained their crystalline structure as revealed by XRD and their interactions with the polymer matrix was evaluated by FTIR.
26485926	3	62	theme	AFM	371:373	arg1	images					375:380	AFM images	371:380	AFM images	371:380	AFM images revealed the egg-box model organization of calcium cross-linked pectin chains used as a matrix.
26485926	0	63	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro and In Vivo Evaluation of Pectin/Copper Exchanged Faujasite Composite Membranes.
26485926	6	64	theme	embedded	736:743	arg1	particles					755:763	The embedded faujasite particles	732:763	The embedded faujasite particles	732:763	The embedded faujasite particles maintained their crystalline structure as revealed by XRD and their interactions with the polymer matrix was evaluated by FTIR.
26485926	1	65	theme	faujasites	152:161	arg1	candidates					169:178	faujasites ideal candidates	152:178	faujasites ideal candidates for tissue engineering applications	152:214	The biocompatibility and excellent ion exchange capacity make faujasites ideal candidates for tissue engineering applications.
26485926	8	66	theme	cell	1097:1100	arg1	viability					1102:1110	cell viability	1097:1110	cell viability of 89%	1097:1117	Finally, it displayed cell viability of 89% on NIH3T3 fibroblast cell lines and aided in improving wound healing and re-epithelialisation in Sprague Dawley rats.
26485926	8	67	theme	NIH3T3	1122:1127	arg1	lines					1145:1149	NIH3T3 fibroblast cell lines	1122:1149	NIH3T3 fibroblast cell lines	1122:1149	Finally, it displayed cell viability of 89% on NIH3T3 fibroblast cell lines and aided in improving wound healing and re-epithelialisation in Sprague Dawley rats.
25620795	4	0	theme	encapsulated	536:547	arg1	cells					554:558	encapsulated beta cells	536:558	encapsulated beta cells	536:558	The potential of this system to enhance the protection of encapsulated beta cells was evaluated.
25620795	3	1	theme	enzyme-mimetic	343:356	arg1	material					358:365	This enzyme-mimetic material	338:365	This enzyme-mimetic material	338:365	This enzyme-mimetic material ubiquitously scavenges ambient free radicals, with the potential to provide indefinite antioxidant protection.
25620795	5	2	theme	potent	644:649	arg1	cytoprotection					651:664	potent cytoprotection	644:664	potent cytoprotection from superoxide exposure	644:689	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	1	3	theme	cellular	176:183	arg1	transplantation					185:199	cellular transplantation	176:199	cellular transplantation	176:199	Oxidative stress and the resulting radical by-products cause significant toxicity and graft loss in cellular transplantation.
25620795	3	4	theme	free	398:401	arg1	radicals					403:410	ambient free radicals	390:410	ambient free radicals	390:410	This enzyme-mimetic material ubiquitously scavenges ambient free radicals, with the potential to provide indefinite antioxidant protection.
25620795	4	5	theme	cells	554:558	arg1	protection					522:531	the protection	518:531	the protection of encapsulated beta cells	518:558	The potential of this system to enhance the protection of encapsulated beta cells was evaluated.
25620795	7	6	theme	sustained	1172:1180	arg1	scavenging					1193:1202	sustained, long-term scavenging	1172:1202	sustained, long-term scavenging	1172:1202	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	7	7	theme	wide	1025:1028	arg1	applicability					1030:1042	wide applicability	1025:1042	wide applicability in cellular transplantation	1025:1070	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	2	8	theme	-composite	305:314	arg1	hydrogel					316:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	Here, the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel is reported.
25620795	5	9	theme	CONPs	721:725	arg1	phagocytosis					701:712	phagocytosis	701:712	phagocytosis of the CONPs by the beta cells	701:743	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	7	10	theme	nanocomposite	998:1010	arg1	hydrogel					1012:1019	This nanocomposite hydrogel	993:1019	This nanocomposite hydrogel	993:1019	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	1	11	theme	Oxidative	76:84	arg1	stress					86:91	Oxidative stress	76:91	Oxidative stress	76:91	Oxidative stress and the resulting radical by-products cause significant toxicity and graft loss in cellular transplantation.
25620795	5	12	theme	low	791:793	arg1	concentrations					773:786	concentrations	773:786	concentrations as low as 1mM	773:800	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	7	13	theme	unique	1082:1087	arg1	advantage					1089:1097	the unique advantage	1078:1097	the unique advantage of localization of these potent antioxidant CONPs	1078:1147	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	5	14	theme	superoxide	671:680	arg1	exposure					682:689	superoxide exposure	671:689	superoxide exposure	671:689	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	2	15	theme	CONP	300:303	arg1	hydrogel					316:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	Here, the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel is reported.
25620795	7	16	from	applicability	1030:1042	arg1	transplantation					1056:1070	cellular transplantation	1047:1070	cellular transplantation	1047:1070	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	3	17	theme	ambient	390:396	arg1	radicals					403:410	ambient free radicals	390:410	ambient free radicals	390:410	This enzyme-mimetic material ubiquitously scavenges ambient free radicals, with the potential to provide indefinite antioxidant protection.
25620795	6	18	theme	radical	939:945	arg1	attack					947:952	free radical attack	934:952	free radical attack	934:952	When CONPs were embedded within alginate hydrogels, the composite hydrogel provided cytoprotection to encapsulated beta cells from free radical attack without cytotoxicity, even up to 10mM.
25620795	6	19	from	attack	947:952	arg1	cells					923:927	encapsulated beta cells	905:927	encapsulated beta cells from free radical attack	905:952	When CONPs were embedded within alginate hydrogels, the composite hydrogel provided cytoprotection to encapsulated beta cells from free radical attack without cytotoxicity, even up to 10mM.
25620795	6	20	theme	encapsulated	905:916	arg1	cells					923:927	encapsulated beta cells	905:927	encapsulated beta cells from free radical attack	905:952	When CONPs were embedded within alginate hydrogels, the composite hydrogel provided cytoprotection to encapsulated beta cells from free radical attack without cytotoxicity, even up to 10mM.
25620795	0	21	theme	cerium	12:17	arg1	nanoparticle					25:36	Antioxidant cerium oxide nanoparticle	0:36	Antioxidant cerium oxide nanoparticle	0:36	Antioxidant cerium oxide nanoparticle hydrogels for cellular encapsulation.
25620795	6	22	theme	free	934:937	arg1	attack					947:952	free radical attack	934:952	free radical attack	934:952	When CONPs were embedded within alginate hydrogels, the composite hydrogel provided cytoprotection to encapsulated beta cells from free radical attack without cytotoxicity, even up to 10mM.
25620795	5	23	theme	beta	620:623	arg1	cells					625:629	beta cells	620:629	beta cells	620:629	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	0	24	theme	Antioxidant	0:10	arg1	nanoparticle					25:36	Antioxidant cerium oxide nanoparticle	0:36	Antioxidant cerium oxide nanoparticle	0:36	Antioxidant cerium oxide nanoparticle hydrogels for cellular encapsulation.
25620795	1	25	theme	resulting	101:109	arg1	by-products					119:129	the resulting radical by-products	97:129	the resulting radical by-products	97:129	Oxidative stress and the resulting radical by-products cause significant toxicity and graft loss in cellular transplantation.
25620795	7	26	theme	antioxidant	1131:1141	arg1	CONPs					1143:1147	these potent antioxidant CONPs	1118:1147	these potent antioxidant CONPs	1118:1147	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	1	27	theme	radical	111:117	arg1	by-products					119:129	the resulting radical by-products	97:129	the resulting radical by-products	97:129	Oxidative stress and the resulting radical by-products cause significant toxicity and graft loss in cellular transplantation.
25620795	6	28	theme	composite	859:867	arg1	hydrogel					869:876	the composite hydrogel	855:876	the composite hydrogel	855:876	When CONPs were embedded within alginate hydrogels, the composite hydrogel provided cytoprotection to encapsulated beta cells from free radical attack without cytotoxicity, even up to 10mM.
25620795	5	29	from	solution	606:613	arg1	free					598:601	free	598:601	free	598:601	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	0	30	theme	oxide	19:23	arg1	nanoparticle					25:36	Antioxidant cerium oxide nanoparticle	0:36	Antioxidant cerium oxide nanoparticle	0:36	Antioxidant cerium oxide nanoparticle hydrogels for cellular encapsulation.
25620795	5	31	from	exposure	682:689	arg1	cytoprotection					651:664	potent cytoprotection	644:664	potent cytoprotection from superoxide exposure	644:689	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	7	32	theme	CONPs	1143:1147	arg1	localization					1102:1113	localization	1102:1113	localization of these potent antioxidant CONPs	1102:1147	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	6	33	dep	10mM	987:990	arg1	up					981:982	up	981:982	up	981:982	When CONPs were embedded within alginate hydrogels, the composite hydrogel provided cytoprotection to encapsulated beta cells from free radical attack without cytotoxicity, even up to 10mM.
25620795	2	34	theme	hydrogel	316:323	arg1	engineering					212:222	the engineering	208:222	the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	208:323	Here, the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel is reported.
25620795	2	35	dep	auto-catalytic	230:243	arg1	antioxidant					246:256	antioxidant	246:256	antioxidant	246:256	Here, the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel is reported.
25620795	2	35	dep	auto-catalytic	230:243	arg1	self-renewing					259:271	self-renewing	259:271	self-renewing	259:271	Here, the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel is reported.
25620795	3	36	theme	indefinite	443:452	arg1	protection					466:475	indefinite antioxidant protection	443:475	indefinite antioxidant protection	443:475	This enzyme-mimetic material ubiquitously scavenges ambient free radicals, with the potential to provide indefinite antioxidant protection.
25620795	7	37	contain	has	1021:1023	arg1	hydrogel					1012:1019	This nanocomposite hydrogel	993:1019	This nanocomposite hydrogel	993:1019	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	7	37	contain	has	1021:1023	arg2	applicability					1030:1042	wide applicability	1025:1042	wide applicability in cellular transplantation	1025:1070	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	3	38	theme	antioxidant	454:464	arg1	protection					466:475	indefinite antioxidant protection	443:475	indefinite antioxidant protection	443:475	This enzyme-mimetic material ubiquitously scavenges ambient free radicals, with the potential to provide indefinite antioxidant protection.
25620795	7	39	theme	potent	1124:1129	arg1	CONPs					1143:1147	these potent antioxidant CONPs	1118:1147	these potent antioxidant CONPs	1118:1147	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	6	40	theme	beta	918:921	arg1	cells					923:927	encapsulated beta cells	905:927	encapsulated beta cells from free radical attack	905:952	When CONPs were embedded within alginate hydrogels, the composite hydrogel provided cytoprotection to encapsulated beta cells from free radical attack without cytotoxicity, even up to 10mM.
25620795	5	41	theme	CONPs	592:596	arg1	Co-incubation					575:587	Co-incubation	575:587	Co-incubation of CONPs free in solution with beta cells	575:629	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	5	42	from	free	598:601	arg1	solution					606:613	solution	606:613	solution	606:613	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	7	43	theme	localization	1102:1113	arg1	capacity					1159:1166	their capacity	1153:1166	their capacity for sustained, long-term scavenging	1153:1202	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	7	43	theme	localization	1102:1113	arg1	advantage					1089:1097	the unique advantage	1078:1097	the unique advantage of localization of these potent antioxidant CONPs	1078:1147	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	4	44	theme	system	500:505	arg1	potential					482:490	The potential	478:490	The potential of this system to enhance the protection of encapsulated beta cells	478:558	The potential of this system to enhance the protection of encapsulated beta cells was evaluated.
25620795	5	45	theme	free	598:601	arg1	CONPs					592:596	CONPs	592:596	CONPs free in solution	592:613	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	0	46	theme	cellular	52:59	arg1	encapsulation					61:73	cellular encapsulation	52:73	cellular encapsulation	52:73	Antioxidant cerium oxide nanoparticle hydrogels for cellular encapsulation.
25620795	2	47	theme	auto-catalytic	230:243	arg1	hydrogel					316:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	Here, the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel is reported.
25620795	1	48	theme	significant	137:147	arg1	toxicity					149:156	significant toxicity	137:156	significant toxicity	137:156	Oxidative stress and the resulting radical by-products cause significant toxicity and graft loss in cellular transplantation.
25620795	5	49	theme	beta	734:737	arg1	cells					739:743	the beta cells	730:743	the beta cells	730:743	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	6	50	theme	alginate	835:842	arg1	hydrogels					844:852	alginate hydrogels	835:852	alginate hydrogels	835:852	When CONPs were embedded within alginate hydrogels, the composite hydrogel provided cytoprotection to encapsulated beta cells from free radical attack without cytotoxicity, even up to 10mM.
25620795	2	51	theme	nanoparticle	286:297	arg1	hydrogel					316:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	Here, the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel is reported.
25620795	5	52	with	Co-incubation	575:587	arg1	cells					625:629	beta cells	620:629	beta cells	620:629	Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1mM.
25620795	2	53	theme	oxide	280:284	arg1	hydrogel					316:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	Here, the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel is reported.
25620795	7	54	theme	cellular	1047:1054	arg1	transplantation					1056:1070	cellular transplantation	1047:1070	cellular transplantation	1047:1070	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
25620795	2	55	theme	cerium	273:278	arg1	hydrogel					316:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel	227:323	Here, the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel is reported.
25620795	1	56	theme	graft	162:166	arg1	loss					168:171	graft loss	162:171	graft loss	162:171	Oxidative stress and the resulting radical by-products cause significant toxicity and graft loss in cellular transplantation.
25620795	4	57	theme	beta	549:552	arg1	cells					554:558	encapsulated beta cells	536:558	encapsulated beta cells	536:558	The potential of this system to enhance the protection of encapsulated beta cells was evaluated.
25620795	7	58	dep	sustained	1172:1180	arg1	long-term					1183:1191	long-term	1183:1191	long-term	1183:1191	This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.
24300948	3	0	theme	chondrogenic	999:1010	arg1	maturities					1012:1021	corresponding chondrogenic maturities	985:1021	corresponding chondrogenic maturities	985:1021	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	8	1	from	cells	2089:2093	arg1	composite					2123:2131	a bilayered OPF hydrogel composite	2098:2131	a bilayered OPF hydrogel composite	2098:2131	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	9	2	theme	chondrogenic	2258:2269	arg1	phenotypes					2286:2295	the respective chondrogenic and osteogenic phenotypes	2243:2295	the respective chondrogenic and osteogenic phenotypes	2243:2295	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	8	3	theme	chondrogenic	1962:1973	arg1	predifferentiation					1975:1992	chondrogenic predifferentiation	1962:1992	chondrogenic predifferentiation	1962:1992	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	2	4	theme	respective	676:685	arg1	phenotypes					687:696	their respective phenotypes	670:696	their respective phenotypes	670:696	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	5	5	theme	higher	1488:1493	arg1	ratios					1531:1536	higher glycosaminoglycan-to-hydroxyproline ratios	1488:1536	higher glycosaminoglycan-to-hydroxyproline ratios	1488:1536	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	3	6	theme	collagen/type	1071:1083	arg1	collagen					1087:1094	type II collagen/type I collagen	1063:1094	type II collagen/type I collagen	1063:1094	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	6	7	theme	higher	1633:1638	arg1	cellularity					1640:1650	higher cellularity	1633:1650	higher cellularity	1633:1650	Osteogenic cells that were co-cultured with chondrogenic cells (in the chondral layer) showed higher cellularity over time, suggesting that chondrogenic cells stimulated the proliferation of osteogenic cells.
24300948	1	8	theme	predifferentiation	232:249	arg1	ability					193:199	the ability	189:199	the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites	189:442	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	2	9	theme	controlled	587:596	arg1	manner					598:603	a spatially controlled manner	575:603	a spatially controlled manner within bilayered constructs	575:631	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	3	10	theme	type	1063:1066	arg1	collagen					1087:1094	type II collagen/type I collagen	1063:1094	type II collagen/type I collagen	1063:1094	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	8	11	theme	native	2034:2039	arg1	cartilage					2049:2057	native hyaline cartilage	2034:2057	native hyaline cartilage	2034:2057	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	1	12	theme	mesenchymal	254:264	arg1	MSCs					278:281	MSCs	278:281	MSCs	278:281	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	1	12	theme	mesenchymal	254:264	arg1	cells					271:275	mesenchymal stem cells	254:275	mesenchymal stem cells (MSCs)	254:282	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	7	13	theme	osteogenic	1760:1769	arg1	cells					1771:1775	osteogenic cells	1760:1775	osteogenic cells	1760:1775	Groups with osteogenic cells displayed mineralization in the subchondral layer, confirming the effect of osteogenic predifferentiation.
24300948	8	14	theme	cartilage	2049:2057	arg1	phenotype					2021:2029	the phenotype	2017:2029	the phenotype of native hyaline cartilage	2017:2057	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	1	15	theme	cells	271:275	arg1	predifferentiation					232:249	chondrogenic and osteogenic predifferentiation	204:249	chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs)	204:282	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	4	16	theme	hydrogel	1232:1239	arg1	composites					1241:1250	bilayered hydrogel composites	1222:1250	bilayered hydrogel composites	1222:1250	Chondrogenic and osteogenic cells were then encapsulated within their respective (chondral/subchondral) layers in bilayered hydrogel composites to include four experimental groups.
24300948	5	17	theme	chondrogenic	1357:1368	arg1	phenotype					1370:1378	enhanced chondrogenic phenotype	1348:1378	enhanced chondrogenic phenotype	1348:1378	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	9	18	theme	external	2355:2362	arg1	factors					2371:2377	external growth factors	2355:2377	external growth factors	2355:2377	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	8	19	theme	osteogenic	2078:2087	arg1	cells					2089:2093	osteogenic cells	2078:2093	osteogenic cells in a bilayered OPF hydrogel composite	2078:2131	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	2	20	theme	factors	784:790	arg1	influence					755:763	the influence	751:763	the influence of external growth factors	751:790	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	1	21	theme	bilayered	370:378	arg1	composites					433:442	injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites	359:442	injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites	359:442	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	6	22	theme	Osteogenic	1539:1548	arg1	cells					1550:1554	Osteogenic cells	1539:1554	Osteogenic cells that were co-cultured with chondrogenic cells (in the chondral layer)	1539:1624	Osteogenic cells that were co-cultured with chondrogenic cells (in the chondral layer) showed higher cellularity over time, suggesting that chondrogenic cells stimulated the proliferation of osteogenic cells.
24300948	7	23	theme	osteogenic	1853:1862	arg1	predifferentiation					1864:1881	osteogenic predifferentiation	1853:1881	osteogenic predifferentiation	1853:1881	Groups with osteogenic cells displayed mineralization in the subchondral layer, confirming the effect of osteogenic predifferentiation.
24300948	2	24	theme	external	768:775	arg1	factors					784:790	external growth factors	768:790	external growth factors	768:790	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	5	25	theme	chondral	1323:1330	arg1	layer					1332:1336	the chondral layer	1319:1336	the chondral layer	1319:1336	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	0	26	theme	mesenchymal	105:115	arg1	cells					122:126	chondrogenically and osteogenically predifferentiated mesenchymal stem cells	51:126	chondrogenically and osteogenically predifferentiated mesenchymal stem cells	51:126	Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels.
24300948	2	27	theme	lineages	563:570	arg1	populations					515:525	cell populations	510:525	cell populations of both chondrogenic and osteogenic lineages	510:570	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	3	28	theme	7	871:871	arg1	days					893:896	7 (CG7) and 14 (CG14) days	871:896	7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3	871:922	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	8	29	theme	hydrogel	2114:2121	arg1	composite					2123:2131	a bilayered OPF hydrogel composite	2098:2131	a bilayered OPF hydrogel composite	2098:2131	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	3	30	theme	exposure	905:912	arg1	days					893:896	7 (CG7) and 14 (CG14) days	871:896	7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3	871:922	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	8	31	theme	important	2201:2209	arg1	duration					2154:2161	the duration	2150:2161	the duration of chondrogenic preconditioning	2150:2193	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	8	31	theme	important	2201:2209	arg1	factor					2211:2216	an important factor	2198:2216	an important factor	2198:2216	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	2	32	theme	factors	730:736	arg1	exchange					706:713	the exchange	702:713	the exchange of biochemical factors	702:736	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	5	33	theme	stronger	1429:1436	arg1	expression					1473:1482	stronger type II collagen and aggrecan gene expression	1429:1482	stronger type II collagen and aggrecan gene expression	1429:1482	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	9	34	theme	novel	2425:2429	arg1	strategy					2431:2438	this novel strategy	2420:2438	this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications	2420:2531	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	6	35	theme	cells	1741:1745	arg1	proliferation					1713:1725	the proliferation	1709:1725	the proliferation of osteogenic cells	1709:1745	Osteogenic cells that were co-cultured with chondrogenic cells (in the chondral layer) showed higher cellularity over time, suggesting that chondrogenic cells stimulated the proliferation of osteogenic cells.
24300948	0	36	theme	osteochondral	14:26	arg1	constructs					35:44	osteochondral tissue constructs	14:44	osteochondral tissue constructs	14:44	Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels.
24300948	1	37	dep	oligo	380:384	arg1	fumarate					408:415	poly(ethylene glycol) fumarate	386:415	poly(ethylene glycol) fumarate	386:415	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	1	37	dep	oligo	380:384	arg1	OPF					419:421	OPF	419:421	OPF	419:421	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	5	38	theme	cell	1403:1406	arg1	populations					1408:1418	other cell populations	1397:1418	other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios	1397:1536	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	0	39	theme	constructs	35:44	arg1	Generation					0:9	Generation	0:9	Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells	0:126	Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels.
24300948	1	40	theme	tissue	335:340	arg1	constructs					342:351	osteochondral tissue constructs	321:351	osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites	321:442	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	8	41	theme	chondrogenic	2166:2177	arg1	preconditioning					2179:2193	chondrogenic preconditioning	2166:2193	chondrogenic preconditioning	2166:2193	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	3	42	theme	populations	968:978	arg1	generation					940:949	the generation	936:949	the generation of distinct cell populations	936:978	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	5	43	theme	gene	1468:1471	arg1	expression					1473:1482	stronger type II collagen and aggrecan gene expression	1429:1482	stronger type II collagen and aggrecan gene expression	1429:1482	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	9	44	theme	osteochondral	2462:2474	arg1	constructs					2483:2492	osteochondral tissue constructs	2462:2492	osteochondral tissue constructs for cartilage engineering applications	2462:2531	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	3	45	theme	distinct	954:961	arg1	populations					968:978	distinct cell populations	954:978	distinct cell populations	954:978	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	0	46	with	Generation	0:9	arg1	cells					122:126	chondrogenically and osteogenically predifferentiated mesenchymal stem cells	51:126	chondrogenically and osteogenically predifferentiated mesenchymal stem cells	51:126	Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels.
24300948	9	47	theme	constructs	2483:2492	arg1	generation					2448:2457	the generation	2444:2457	the generation of osteochondral tissue constructs for cartilage engineering applications	2444:2531	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	2	48	theme	growth	777:782	arg1	factors					784:790	external growth factors	768:790	external growth factors	768:790	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	5	49	theme	Encapsulated	1289:1300	arg1	cells					1306:1310	Encapsulated CG7 cells	1289:1310	Encapsulated CG7 cells within the chondral layer	1289:1336	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	9	50	theme	cartilage	2498:2506	arg1	applications					2520:2531	cartilage engineering applications	2498:2531	cartilage engineering applications	2498:2531	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	3	51	dep	aggrecan	1050:1057	arg1	expression					1096:1105	expression	1096:1105	expression	1096:1105	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	3	52	theme	corresponding	985:997	arg1	maturities					1012:1021	corresponding chondrogenic maturities	985:1021	corresponding chondrogenic maturities	985:1021	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	8	53	theme	OPF	2110:2112	arg1	composite					2123:2131	a bilayered OPF hydrogel composite	2098:2131	a bilayered OPF hydrogel composite	2098:2131	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	8	54	theme	predifferentiation	1975:1992	arg1	MSCs					1914:1917	MSCs	1914:1917	MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation	1914:1992	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	6	55	theme	chondrogenic	1583:1594	arg1	cells					1596:1600	chondrogenic cells	1583:1600	chondrogenic cells (in the chondral layer)	1583:1624	Osteogenic cells that were co-cultured with chondrogenic cells (in the chondral layer) showed higher cellularity over time, suggesting that chondrogenic cells stimulated the proliferation of osteogenic cells.
24300948	9	56	theme	osteogenic	2275:2284	arg1	phenotypes					2286:2295	the respective chondrogenic and osteogenic phenotypes	2243:2295	the respective chondrogenic and osteogenic phenotypes	2243:2295	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	4	57	theme	respective	1178:1187	arg1	layers					1212:1217	their respective (chondral/subchondral) layers	1172:1217	their respective (chondral/subchondral) layers in bilayered hydrogel composites	1172:1250	Chondrogenic and osteogenic cells were then encapsulated within their respective (chondral/subchondral) layers in bilayered hydrogel composites to include four experimental groups.
24300948	7	58	theme	predifferentiation	1864:1881	arg1	effect					1843:1848	the effect	1839:1848	the effect of osteogenic predifferentiation	1839:1881	Groups with osteogenic cells displayed mineralization in the subchondral layer, confirming the effect of osteogenic predifferentiation.
24300948	1	59	theme	chondrogenic	204:215	arg1	predifferentiation					232:249	chondrogenic and osteogenic predifferentiation	204:249	chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs)	204:282	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	4	60	theme	chondral/subchondral	1190:1209	arg1	layers					1212:1217	their respective (chondral/subchondral) layers	1172:1217	their respective (chondral/subchondral) layers in bilayered hydrogel composites	1172:1250	Chondrogenic and osteogenic cells were then encapsulated within their respective (chondral/subchondral) layers in bilayered hydrogel composites to include four experimental groups.
24300948	8	61	theme	bilayered	2100:2108	arg1	composite					2123:2131	a bilayered OPF hydrogel composite	2098:2131	a bilayered OPF hydrogel composite	2098:2131	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	5	62	theme	glycosaminoglycan-to-hydroxyproline	1495:1529	arg1	ratios					1531:1536	higher glycosaminoglycan-to-hydroxyproline ratios	1488:1536	higher glycosaminoglycan-to-hydroxyproline ratios	1488:1536	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	1	63	theme	osteogenic	221:230	arg1	predifferentiation					232:249	chondrogenic and osteogenic predifferentiation	204:249	chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs)	204:282	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	3	64	theme	prior	820:824	arg1	expansion					810:818	monolayer expansion	800:818	monolayer expansion prior to hydrogel encapsulation	800:850	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	8	65	theme	hyaline	2041:2047	arg1	cartilage					2049:2057	native hyaline cartilage	2034:2057	native hyaline cartilage	2034:2057	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	8	66	located	found	1903:1907	arg2	it					1896:1897	it	1896:1897	it	1896:1897	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	8	66	located	found	1903:1907	arg1	summary					1887:1893	summary	1887:1893	summary	1887:1893	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	3	67	theme	monolayer	800:808	arg1	expansion					810:818	monolayer expansion	800:818	monolayer expansion prior to hydrogel encapsulation	800:850	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	1	68	theme	stem	266:269	arg1	MSCs					278:281	MSCs	278:281	MSCs	278:281	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	1	68	theme	stem	266:269	arg1	cells					271:275	mesenchymal stem cells	254:275	mesenchymal stem cells (MSCs)	254:282	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	2	69	theme	bilayered	612:620	arg1	constructs					622:631	bilayered constructs	612:631	bilayered constructs	612:631	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	5	70	theme	enhanced	1348:1355	arg1	phenotype					1370:1378	enhanced chondrogenic phenotype	1348:1378	enhanced chondrogenic phenotype	1348:1378	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	3	71	theme	hydrogel	829:836	arg1	encapsulation					838:850	hydrogel encapsulation	829:850	hydrogel encapsulation	829:850	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	1	72	theme	hydrogel	424:431	arg1	composites					433:442	injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites	359:442	injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites	359:442	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	9	73	theme	growth	2364:2369	arg1	factors					2371:2377	external growth factors	2355:2377	external growth factors	2355:2377	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	0	74	theme	predifferentiated	87:103	arg1	cells					122:126	chondrogenically and osteogenically predifferentiated mesenchymal stem cells	51:126	chondrogenically and osteogenically predifferentiated mesenchymal stem cells	51:126	Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels.
24300948	6	75	theme	chondrogenic	1679:1690	arg1	cells					1692:1696	chondrogenic cells	1679:1696	chondrogenic cells	1679:1696	Osteogenic cells that were co-cultured with chondrogenic cells (in the chondral layer) showed higher cellularity over time, suggesting that chondrogenic cells stimulated the proliferation of osteogenic cells.
24300948	3	76	theme	14	883:884	arg1	days					893:896	7 (CG7) and 14 (CG14) days	871:896	7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3	871:922	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	9	77	theme	exciting	2398:2405	arg1	potential					2407:2415	the exciting potential	2394:2415	the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications	2394:2531	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	4	78	theme	bilayered	1222:1230	arg1	composites					1241:1250	bilayered hydrogel composites	1222:1250	bilayered hydrogel composites	1222:1250	Chondrogenic and osteogenic cells were then encapsulated within their respective (chondral/subchondral) layers in bilayered hydrogel composites to include four experimental groups.
24300948	0	79	theme	stem	117:120	arg1	cells					122:126	chondrogenically and osteogenically predifferentiated mesenchymal stem cells	51:126	chondrogenically and osteogenically predifferentiated mesenchymal stem cells	51:126	Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels.
24300948	3	80	theme	MSC	901:903	arg1	exposure					905:912	MSC exposure	901:912	MSC exposure to TGF-β3	901:922	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	2	81	theme	cell	510:513	arg1	populations					515:525	cell populations	510:525	cell populations of both chondrogenic and osteogenic lineages	510:570	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	1	82	theme	poly	386:389	arg1	fumarate					408:415	poly(ethylene glycol) fumarate	386:415	poly(ethylene glycol) fumarate	386:415	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	2	83	theme	osteogenic	552:561	arg1	lineages					563:570	both chondrogenic and osteogenic lineages	530:570	both chondrogenic and osteogenic lineages	530:570	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	0	84	theme	bilayered	144:152	arg1	hydrogels					154:162	bilayered hydrogels	144:162	bilayered hydrogels	144:162	Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels.
24300948	1	85	theme	ethylene	391:398	arg1	poly					386:389	poly	386:389	poly(ethylene glycol) fumarate	386:415	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	1	85	theme	ethylene	391:398	arg1	glycol					400:405	ethylene glycol	391:405	ethylene glycol	391:405	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	7	86	with	Groups	1748:1753	arg1	cells					1771:1775	osteogenic cells	1760:1775	osteogenic cells	1760:1775	Groups with osteogenic cells displayed mineralization in the subchondral layer, confirming the effect of osteogenic predifferentiation.
24300948	2	87	theme	chondrogenic	535:546	arg1	lineages					563:570	both chondrogenic and osteogenic lineages	530:570	both chondrogenic and osteogenic lineages	530:570	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	4	88	theme	experimental	1268:1279	arg1	groups					1281:1286	four experimental groups	1263:1286	four experimental groups	1263:1286	Chondrogenic and osteogenic cells were then encapsulated within their respective (chondral/subchondral) layers in bilayered hydrogel composites to include four experimental groups.
24300948	2	89	theme	biochemical	718:728	arg1	factors					730:736	biochemical factors	718:736	biochemical factors	718:736	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	9	90	theme	strategy	2431:2438	arg1	potential					2407:2415	the exciting potential	2394:2415	the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications	2394:2531	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	0	91	theme	tissue	28:33	arg1	constructs					35:44	osteochondral tissue constructs	14:44	osteochondral tissue constructs	14:44	Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels.
24300948	4	92	from	layers	1212:1217	arg1	composites					1241:1250	bilayered hydrogel composites	1222:1250	bilayered hydrogel composites	1222:1250	Chondrogenic and osteogenic cells were then encapsulated within their respective (chondral/subchondral) layers in bilayered hydrogel composites to include four experimental groups.
24300948	6	93	theme	osteogenic	1730:1739	arg1	cells					1741:1745	osteogenic cells	1730:1745	osteogenic cells	1730:1745	Osteogenic cells that were co-cultured with chondrogenic cells (in the chondral layer) showed higher cellularity over time, suggesting that chondrogenic cells stimulated the proliferation of osteogenic cells.
24300948	7	94	theme	subchondral	1809:1819	arg1	layer					1821:1825	the subchondral layer	1805:1825	the subchondral layer	1805:1825	Groups with osteogenic cells displayed mineralization in the subchondral layer, confirming the effect of osteogenic predifferentiation.
24300948	1	95	dep	bilayered	370:378	arg1	oligo					380:384	oligo	380:384	oligo	380:384	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	5	96	theme	other	1397:1401	arg1	populations					1408:1418	other cell populations	1397:1418	other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios	1397:1536	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	8	97	theme	preconditioning	2179:2193	arg1	duration					2154:2161	the duration	2150:2161	the duration of chondrogenic preconditioning	2150:2193	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	8	97	theme	preconditioning	2179:2193	arg1	factor					2211:2216	an important factor	2198:2216	an important factor	2198:2216	In summary, it was found that MSCs that underwent 7 days, but not 14 days, of chondrogenic predifferentiation most closely resembled the phenotype of native hyaline cartilage when combined with osteogenic cells in a bilayered OPF hydrogel composite, indicating that the duration of chondrogenic preconditioning is an important factor to control.
24300948	1	98	theme	osteochondral	321:333	arg1	constructs					342:351	osteochondral tissue constructs	321:351	osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites	321:442	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	5	99	theme	aggrecan	1459:1466	arg1	expression					1473:1482	stronger type II collagen and aggrecan gene expression	1429:1482	stronger type II collagen and aggrecan gene expression	1429:1482	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	1	100	theme	constructs	342:351	arg1	development					306:316	the development	302:316	the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites	302:442	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	2	101	theme	combinatorial	470:482	arg1	approach					484:491	the combinatorial approach	466:491	the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs	466:631	We hypothesized that the combinatorial approach of encapsulating cell populations of both chondrogenic and osteogenic lineages in a spatially controlled manner within bilayered constructs would enable these cells to maintain their respective phenotypes via the exchange of biochemical factors even without the influence of external growth factors.
24300948	9	102	theme	tissue	2476:2481	arg1	constructs					2483:2492	osteochondral tissue constructs	2462:2492	osteochondral tissue constructs for cartilage engineering applications	2462:2531	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	6	103	theme	chondral	1610:1617	arg1	layer					1619:1623	the chondral layer	1606:1623	the chondral layer	1606:1623	Osteogenic cells that were co-cultured with chondrogenic cells (in the chondral layer) showed higher cellularity over time, suggesting that chondrogenic cells stimulated the proliferation of osteogenic cells.
24300948	1	104	theme	injectable	359:368	arg1	composites					433:442	injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites	359:442	injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites	359:442	This study investigated the ability of chondrogenic and osteogenic predifferentiation of mesenchymal stem cells (MSCs) to play a role in the development of osteochondral tissue constructs using injectable bilayered oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites.
24300948	3	105	theme	cell	963:966	arg1	populations					968:978	distinct cell populations	954:978	distinct cell populations	954:978	During monolayer expansion prior to hydrogel encapsulation, it was found that 7 (CG7) and 14 (CG14) days of MSC exposure to TGF-β3 allowed for the generation of distinct cell populations with corresponding chondrogenic maturities as indicated by increasing aggrecan and type II collagen/type I collagen expression.
24300948	4	106	theme	Chondrogenic	1108:1119	arg1	cells					1136:1140	Chondrogenic and osteogenic cells	1108:1140	Chondrogenic and osteogenic cells	1108:1140	Chondrogenic and osteogenic cells were then encapsulated within their respective (chondral/subchondral) layers in bilayered hydrogel composites to include four experimental groups.
24300948	5	107	theme	type	1438:1441	arg1	collagen					1446:1453	type II collagen	1438:1453	type II collagen	1438:1453	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	5	108	theme	CG7	1302:1304	arg1	cells					1306:1310	Encapsulated CG7 cells	1289:1310	Encapsulated CG7 cells within the chondral layer	1289:1336	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	6	109	from	cells	1596:1600	arg1	layer					1619:1623	the chondral layer	1606:1623	the chondral layer	1606:1623	Osteogenic cells that were co-cultured with chondrogenic cells (in the chondral layer) showed higher cellularity over time, suggesting that chondrogenic cells stimulated the proliferation of osteogenic cells.
24300948	4	110	theme	osteogenic	1125:1134	arg1	cells					1136:1140	Chondrogenic and osteogenic cells	1108:1140	Chondrogenic and osteogenic cells	1108:1140	Chondrogenic and osteogenic cells were then encapsulated within their respective (chondral/subchondral) layers in bilayered hydrogel composites to include four experimental groups.
24300948	9	111	theme	respective	2247:2256	arg1	phenotypes					2286:2295	the respective chondrogenic and osteogenic phenotypes	2243:2295	the respective chondrogenic and osteogenic phenotypes	2243:2295	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
24300948	5	112	theme	collagen	1446:1453	arg1	expression					1473:1482	stronger type II collagen and aggrecan gene expression	1429:1482	stronger type II collagen and aggrecan gene expression	1429:1482	Encapsulated CG7 cells within the chondral layer exhibited enhanced chondrogenic phenotype when compared to other cell populations based on stronger type II collagen and aggrecan gene expression and higher glycosaminoglycan-to-hydroxyproline ratios.
24300948	9	113	theme	engineering	2508:2518	arg1	applications					2520:2531	cartilage engineering applications	2498:2531	cartilage engineering applications	2498:2531	Furthermore, the respective chondrogenic and osteogenic phenotypes were maintained for 28 days in vitro without the need for external growth factors, demonstrating the exciting potential of this novel strategy for the generation of osteochondral tissue constructs for cartilage engineering applications.
26478335	0	0	theme	composite	96:104	arg1	hydrogel					106:113	Fmoc-FF composite hydrogel	88:113	Fmoc-FF composite hydrogel	88:113	Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel.
26478335	7	1	theme	Cell	1097:1100	arg1	assay					1112:1116	Cell viability assay	1097:1116	Cell viability assay	1097:1116	Cell viability assay revealed that the Fmoc-FF and Fmoc-FF/SA hydrogels are both beneficial for cell proliferation in-vitro.
26478335	0	2	theme	Fmoc-FF	88:94	arg1	hydrogel					106:113	Fmoc-FF composite hydrogel	88:113	Fmoc-FF composite hydrogel	88:113	Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel.
26478335	5	3	theme	chemical	932:939	arg1	stability					941:949	stability	941:949	physical and chemical stability as well as possessing good biocompatibility	919:993	Our results demonstrated that the formed hydrogel showed physical and chemical stability as well as possessing good biocompatibility.
26478335	6	4	theme	SA	1049:1050	arg1	addition					1037:1044	the addition	1033:1044	the addition of SA	1033:1050	Rheological measurements showed that the addition of SA could improve the stability of the hydrogel.
26478335	3	5	dep	-diphenylalanine	537:552	arg1	Phe-Phe					555:561	Phe-Phe	555:561	Phe-Phe	555:561	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	5	6	theme	formed	896:901	arg1	hydrogel					903:910	the formed hydrogel	892:910	the formed hydrogel	892:910	Our results demonstrated that the formed hydrogel showed physical and chemical stability as well as possessing good biocompatibility.
26478335	7	7	theme	Fmoc-FF/SA	1148:1157	arg1	hydrogels					1159:1167	the Fmoc-FF and Fmoc-FF/SA hydrogels	1132:1167	the Fmoc-FF and Fmoc-FF/SA hydrogels	1132:1167	Cell viability assay revealed that the Fmoc-FF and Fmoc-FF/SA hydrogels are both beneficial for cell proliferation in-vitro.
26478335	7	7	theme	Fmoc-FF/SA	1148:1157	arg1	beneficial					1178:1187	beneficial	1178:1187	beneficial	1178:1187	Cell viability assay revealed that the Fmoc-FF and Fmoc-FF/SA hydrogels are both beneficial for cell proliferation in-vitro.
26478335	3	8	theme	rigid	587:591	arg1	hydrogels					593:601	rigid hydrogels	587:601	rigid hydrogels with structures of nanowires, layered thin films or honeycombs	587:664	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	2	9	theme	composite	445:453	arg1	hydrogel					455:462	a rigid and structure controllable Fmoc-FF/SA composite hydrogel	399:462	a rigid and structure controllable Fmoc-FF/SA composite hydrogel	399:462	In the present study, we dedicate to fabricate a rigid and structure controllable Fmoc-FF/SA composite hydrogel.
26478335	7	10	dep	proliferation	1198:1210	arg1	in-vitro					1212:1219	cell proliferation in-vitro	1193:1219	cell proliferation in-vitro	1193:1219	Cell viability assay revealed that the Fmoc-FF and Fmoc-FF/SA hydrogels are both beneficial for cell proliferation in-vitro.
26478335	1	11	theme	excellent	201:209	arg1	abilities					211:219	their excellent abilities	195:219	their excellent abilities of self-assemble	195:236	Dipeptides and their derivatives have attracted tremendous attention owning to their excellent abilities of self-assemble assembling into various structures which have great potentials for applications in biology and/or nanotechnology.
26478335	8	12	theme	fabricated	1253:1262	arg1	hydrogels					1285:1293	the fabricated Fmoc-FF/SA composite hydrogels	1249:1293	the fabricated Fmoc-FF/SA composite hydrogels	1249:1293	Our results indicated that the fabricated Fmoc-FF/SA composite hydrogels could be used in tissue engineering and drug delivery in the future.
26478335	7	13	theme	Fmoc-FF	1136:1142	arg1	hydrogels					1159:1167	the Fmoc-FF and Fmoc-FF/SA hydrogels	1132:1167	the Fmoc-FF and Fmoc-FF/SA hydrogels	1132:1167	Cell viability assay revealed that the Fmoc-FF and Fmoc-FF/SA hydrogels are both beneficial for cell proliferation in-vitro.
26478335	7	13	theme	Fmoc-FF	1136:1142	arg1	beneficial					1178:1187	beneficial	1178:1187	beneficial	1178:1187	Cell viability assay revealed that the Fmoc-FF and Fmoc-FF/SA hydrogels are both beneficial for cell proliferation in-vitro.
26478335	8	14	used	used	1304:1307	arg2	hydrogels					1285:1293	the fabricated Fmoc-FF/SA composite hydrogels	1249:1293	the fabricated Fmoc-FF/SA composite hydrogels	1249:1293	Our results indicated that the fabricated Fmoc-FF/SA composite hydrogels could be used in tissue engineering and drug delivery in the future.
26478335	2	15	theme	controllable	421:432	arg1	hydrogel					455:462	a rigid and structure controllable Fmoc-FF/SA composite hydrogel	399:462	a rigid and structure controllable Fmoc-FF/SA composite hydrogel	399:462	In the present study, we dedicate to fabricate a rigid and structure controllable Fmoc-FF/SA composite hydrogel.
26478335	8	16	theme	tissue	1312:1317	arg1	engineering					1319:1329	tissue engineering	1312:1329	tissue engineering	1312:1329	Our results indicated that the fabricated Fmoc-FF/SA composite hydrogels could be used in tissue engineering and drug delivery in the future.
26478335	2	17	theme	present	359:365	arg1	study					367:371	the present study	355:371	the present study	355:371	In the present study, we dedicate to fabricate a rigid and structure controllable Fmoc-FF/SA composite hydrogel.
26478335	5	18	theme	physical	919:926	arg1	stability					941:949	stability	941:949	physical and chemical stability as well as possessing good biocompatibility	919:993	Our results demonstrated that the formed hydrogel showed physical and chemical stability as well as possessing good biocompatibility.
26478335	8	19	theme	composite	1275:1283	arg1	hydrogels					1285:1293	the fabricated Fmoc-FF/SA composite hydrogels	1249:1293	the fabricated Fmoc-FF/SA composite hydrogels	1249:1293	Our results indicated that the fabricated Fmoc-FF/SA composite hydrogels could be used in tissue engineering and drug delivery in the future.
26478335	5	20	theme	good	973:976	arg1	biocompatibility					978:993	possessing good biocompatibility	962:993	physical and chemical stability as well as possessing good biocompatibility	919:993	Our results demonstrated that the formed hydrogel showed physical and chemical stability as well as possessing good biocompatibility.
26478335	1	21	theme	self-assemble	224:236	arg1	abilities					211:219	their excellent abilities	195:219	their excellent abilities of self-assemble	195:236	Dipeptides and their derivatives have attracted tremendous attention owning to their excellent abilities of self-assemble assembling into various structures which have great potentials for applications in biology and/or nanotechnology.
26478335	3	22	theme	layered	633:639	arg1	films					646:650	layered thin films	633:650	layered thin films	633:650	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	3	23	theme	nanowires	622:630	arg1	structures					608:617	structures	608:617	structures of nanowires, layered thin films or honeycombs	608:664	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	8	24	theme	drug	1335:1338	arg1	delivery					1340:1347	drug delivery	1335:1347	drug delivery	1335:1347	Our results indicated that the fabricated Fmoc-FF/SA composite hydrogels could be used in tissue engineering and drug delivery in the future.
26478335	0	25	theme	novel	38:42	arg1	alginate					51:58	a novel sodium alginate	36:58	a novel sodium alginate incorporated	36:71	Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel.
26478335	3	26	theme	SA	700:701	arg1	concentration					704:716	sodium alginate (SA) concentration	683:716	sodium alginate (SA) concentration	683:716	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	4	27	theme	peptide	853:859	arg1	arrangement					826:836	the arrangement	822:836	the arrangement of the Fmoc-FF peptide	822:859	Meanwhile, CD-spectroscopy demonstrated that SA appeared to control the process, but it did not change the arrangement of the Fmoc-FF peptide.
26478335	3	28	theme	modified	483:490	arg1	dipeptide					492:500	the modified dipeptide	479:500	the modified dipeptide	479:500	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	3	29	with	hydrogels	593:601	arg1	structures					608:617	structures	608:617	structures of nanowires, layered thin films or honeycombs	608:664	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	3	30	theme	sodium	683:688	arg1	concentration					704:716	sodium alginate (SA) concentration	683:716	sodium alginate (SA) concentration	683:716	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	1	31	contain	have	279:282	arg1	structures					262:271	various structures	254:271	various structures which have great potentials for applications in biology and/or nanotechnology	254:349	Dipeptides and their derivatives have attracted tremendous attention owning to their excellent abilities of self-assemble assembling into various structures which have great potentials for applications in biology and/or nanotechnology.
26478335	1	31	contain	have	279:282	arg2	potentials					290:299	great potentials	284:299	great potentials for applications	284:316	Dipeptides and their derivatives have attracted tremendous attention owning to their excellent abilities of self-assemble assembling into various structures which have great potentials for applications in biology and/or nanotechnology.
26478335	1	32	theme	various	254:260	arg1	structures					262:271	various structures	254:271	various structures which have great potentials for applications in biology and/or nanotechnology	254:349	Dipeptides and their derivatives have attracted tremendous attention owning to their excellent abilities of self-assemble assembling into various structures which have great potentials for applications in biology and/or nanotechnology.
26478335	0	33	theme	alginate	51:58	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel.
26478335	0	33	theme	alginate	51:58	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel.
26478335	6	34	theme	Rheological	996:1006	arg1	measurements					1008:1019	Rheological measurements	996:1019	Rheological measurements	996:1019	Rheological measurements showed that the addition of SA could improve the stability of the hydrogel.
26478335	6	35	theme	hydrogel	1087:1094	arg1	stability					1070:1078	the stability	1066:1078	the stability of the hydrogel	1066:1094	Rheological measurements showed that the addition of SA could improve the stability of the hydrogel.
26478335	3	36	theme	alginate	690:697	arg1	concentration					704:716	sodium alginate (SA) concentration	683:716	sodium alginate (SA) concentration	683:716	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	3	37	theme	thin	641:644	arg1	films					646:650	layered thin films	633:650	layered thin films	633:650	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	0	38	theme	sodium	44:49	arg1	alginate					51:58	a novel sodium alginate	36:58	a novel sodium alginate incorporated	36:71	Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel.
26478335	7	39	theme	viability	1102:1110	arg1	assay					1112:1116	Cell viability assay	1097:1116	Cell viability assay	1097:1116	Cell viability assay revealed that the Fmoc-FF and Fmoc-FF/SA hydrogels are both beneficial for cell proliferation in-vitro.
26478335	3	40	theme	films	646:650	arg1	structures					608:617	structures	608:617	structures of nanowires, layered thin films or honeycombs	608:664	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	4	41	theme	Fmoc-FF	845:851	arg1	peptide					853:859	the Fmoc-FF peptide	841:859	the Fmoc-FF peptide	841:859	Meanwhile, CD-spectroscopy demonstrated that SA appeared to control the process, but it did not change the arrangement of the Fmoc-FF peptide.
26478335	3	42	theme	honeycombs	655:664	arg1	structures					608:617	structures	608:617	structures of nanowires, layered thin films or honeycombs	608:664	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	5	43	theme	possessing	962:971	arg1	biocompatibility					978:993	possessing good biocompatibility	962:993	physical and chemical stability as well as possessing good biocompatibility	919:993	Our results demonstrated that the formed hydrogel showed physical and chemical stability as well as possessing good biocompatibility.
26478335	3	44	theme	-diphenylalanine	537:552	arg1	dipeptide					492:500	the modified dipeptide	479:500	the modified dipeptide	479:500	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	3	45	theme	concentration	704:716	arg1	change					673:678	the change	669:678	the change of sodium alginate (SA) concentration	669:716	We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration.
26478335	1	46	theme	tremendous	164:173	arg1	attention					175:183	tremendous attention	164:183	tremendous attention owning to their excellent abilities of self-assemble	164:236	Dipeptides and their derivatives have attracted tremendous attention owning to their excellent abilities of self-assemble assembling into various structures which have great potentials for applications in biology and/or nanotechnology.
26478335	2	47	theme	Fmoc-FF/SA	434:443	arg1	hydrogel					455:462	a rigid and structure controllable Fmoc-FF/SA composite hydrogel	399:462	a rigid and structure controllable Fmoc-FF/SA composite hydrogel	399:462	In the present study, we dedicate to fabricate a rigid and structure controllable Fmoc-FF/SA composite hydrogel.
26478335	7	48	theme	cell	1193:1196	arg1	proliferation					1198:1210	cell proliferation in-vitro	1193:1219	cell proliferation in-vitro	1193:1219	Cell viability assay revealed that the Fmoc-FF and Fmoc-FF/SA hydrogels are both beneficial for cell proliferation in-vitro.
26478335	8	49	theme	Fmoc-FF/SA	1264:1273	arg1	hydrogels					1285:1293	the fabricated Fmoc-FF/SA composite hydrogels	1249:1293	the fabricated Fmoc-FF/SA composite hydrogels	1249:1293	Our results indicated that the fabricated Fmoc-FF/SA composite hydrogels could be used in tissue engineering and drug delivery in the future.
26478335	2	50	theme	rigid	401:405	arg1	hydrogel					455:462	a rigid and structure controllable Fmoc-FF/SA composite hydrogel	399:462	a rigid and structure controllable Fmoc-FF/SA composite hydrogel	399:462	In the present study, we dedicate to fabricate a rigid and structure controllable Fmoc-FF/SA composite hydrogel.
26478335	1	51	theme	great	284:288	arg1	potentials					290:299	great potentials	284:299	great potentials for applications	284:316	Dipeptides and their derivatives have attracted tremendous attention owning to their excellent abilities of self-assemble assembling into various structures which have great potentials for applications in biology and/or nanotechnology.
25464506	8	0	theme	loaded	1646:1651	arg1	lysostaphin					1653:1663	the loaded lysostaphin	1642:1663	the loaded lysostaphin	1642:1663	Finally the antibacterial activity assay indicated that the loaded lysostaphin had good release ability and strong antibacterial enzymatic activity against methicillin-resistant Staphylococcus aureus.
25464506	7	1	theme	methylthiazol	1406:1418	arg1	assay					1432:1436	the methylthiazol tetrazolium assay	1402:1436	the methylthiazol tetrazolium assay	1402:1436	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	1	2	theme	setting	290:296	arg1	method					298:303	a novel setting method	282:303	a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold	282:360	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	6	3	dep	in	1218:1219	arg1	vivo					1221:1224	vivo	1221:1224	vivo	1221:1224	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	6	4	theme	extract	1111:1117	arg1	experiment					1119:1128	the in vitro cement extract experiment	1091:1128	the in vitro cement extract experiment	1091:1128	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	8	5	theme	release	1674:1680	arg1	ability					1682:1688	good release ability	1669:1688	good release ability	1669:1688	Finally the antibacterial activity assay indicated that the loaded lysostaphin had good release ability and strong antibacterial enzymatic activity against methicillin-resistant Staphylococcus aureus.
25464506	4	6	theme	setting	814:820	arg1	process					822:828	the setting process	810:828	the setting process	810:828	The results showed that the final setting time was 96.6±5.2 min and the pH value increased from approximately 6.2 to nearly 10 during the setting process and the porosity was 34% at the end.
25464506	10	7	theme	artificial	2107:2116	arg1	bone					2118:2121	artificial bone substitute	2107:2132	artificial bone substitute	2107:2132	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	1	8	theme	porous	215:220	arg1	cement					227:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement	147:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery	147:264	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	8	9	theme	antibacterial	1701:1713	arg1	activity					1725:1732	strong antibacterial enzymatic activity	1694:1732	strong antibacterial enzymatic activity	1694:1732	Finally the antibacterial activity assay indicated that the loaded lysostaphin had good release ability and strong antibacterial enzymatic activity against methicillin-resistant Staphylococcus aureus.
25464506	7	10	theme	mice	1580:1583	arg1	cells					1546:1550	MC 3T3-E1 cells	1536:1550	MC 3T3-E1 cells	1536:1550	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	7	10	theme	mice	1580:1583	arg1	tissues					1569:1575	subcutaneous tissues	1556:1575	subcutaneous tissues	1556:1575	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	6	11	theme	protein	1176:1182	arg1	%					1160:1160	about 94.2±10.9%	1145:1160	about 94.2±10.9% of the loaded protein	1145:1182	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	6	11	theme	protein	1176:1182	arg1	protein					1176:1182	the loaded protein	1165:1182	the loaded protein	1165:1182	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	10	12	theme	controlled-release	2138:2155	arg1	system					2157:2162	controlled-release system	2138:2162	controlled-release system for delivery of lysostaphin	2138:2190	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	10	12	theme	controlled-release	2138:2155	arg1	cement					2053:2058	this bone cement	2043:2058	this bone cement	2043:2058	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	6	13	theme	tracking	1248:1255	arg1	experiment					1257:1266	the in vivo Qdot 625 fluorescence tracking experiment	1214:1266	the in vivo Qdot 625 fluorescence tracking experiment	1214:1266	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	4	14	from	end	862:864	arg1	%					853:853	34%	851:853	34%	851:853	The results showed that the final setting time was 96.6±5.2 min and the pH value increased from approximately 6.2 to nearly 10 during the setting process and the porosity was 34% at the end.
25464506	4	14	from	end	862:864	arg1	porosity					838:845	the porosity	834:845	the porosity	834:845	The results showed that the final setting time was 96.6±5.2 min and the pH value increased from approximately 6.2 to nearly 10 during the setting process and the porosity was 34% at the end.
25464506	1	15	theme	efficient	239:247	arg1	delivery					257:264	efficient protein delivery	239:264	efficient protein delivery	239:264	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	2	16	theme	citric	558:563	arg1	acid					565:568	citric acid	558:568	citric acid	558:568	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	5	17	theme	transform-infrared	989:1006	arg1	spectroscopy					1008:1019	Fourier transform-infrared spectroscopy	981:1019	Fourier transform-infrared spectroscopy	981:1019	And the microstructure and composition were detected by scanning electron microscopy (SEM), x-ray diffraction and Fourier transform-infrared spectroscopy.
25464506	7	18	with	samples	1523:1529	arg1	cells					1546:1550	MC 3T3-E1 cells	1536:1550	MC 3T3-E1 cells	1536:1550	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	7	18	with	samples	1523:1529	arg1	tissues					1569:1575	subcutaneous tissues	1556:1575	subcutaneous tissues	1556:1575	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	7	19	theme	3T3-E1	1539:1544	arg1	cells					1546:1550	MC 3T3-E1 cells	1536:1550	MC 3T3-E1 cells	1536:1550	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	4	20	dep	10	800:801	arg1	to					790:791	to	790:791	to	790:791	The results showed that the final setting time was 96.6±5.2 min and the pH value increased from approximately 6.2 to nearly 10 during the setting process and the porosity was 34% at the end.
25464506	10	21	theme	bone	2201:2204	arg1	defects					2206:2212	bone defects	2201:2212	bone defects	2201:2212	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	0	22	theme	hydroxyapatite/chitosan	86:108	arg1	cement					125:130	hydroxyapatite/chitosan composite bone cement	86:130	hydroxyapatite/chitosan composite bone cement	86:130	A novel controlled-release system for antibacterial enzyme lysostaphin delivery using hydroxyapatite/chitosan composite bone cement.
25464506	7	23	theme	cement	1516:1521	arg1	samples					1523:1529	cement samples	1516:1529	cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice	1516:1583	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	0	24	theme	bone	120:123	arg1	cement					125:130	hydroxyapatite/chitosan composite bone cement	86:130	hydroxyapatite/chitosan composite bone cement	86:130	A novel controlled-release system for antibacterial enzyme lysostaphin delivery using hydroxyapatite/chitosan composite bone cement.
25464506	2	25	theme	solid	433:437	arg1	CaCO3					509:513	CaCO3	509:513	CaCO3	509:513	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	25	theme	solid	433:437	arg1	NaHCO3					519:524	NaHCO3	519:524	NaHCO3	519:524	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	25	theme	solid	433:437	arg1	OH					503:504	Ca(OH)2	500:506	Ca(OH)2	500:506	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	25	theme	solid	433:437	arg1	powder					439:444	solid powder	433:444	solid powder	433:444	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	25	theme	solid	433:437	arg1	mixture					449:455	a mixture	447:455	a mixture of HA/CS composite particles	447:484	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	25	theme	solid	433:437	arg1	lysostaphin					487:497	lysostaphin	487:497	lysostaphin	487:497	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	5	26	theme	scanning	923:930	arg1	SEM					953:955	SEM	953:955	SEM	953:955	And the microstructure and composition were detected by scanning electron microscopy (SEM), x-ray diffraction and Fourier transform-infrared spectroscopy.
25464506	5	26	theme	scanning	923:930	arg1	microscopy					941:950	scanning electron microscopy	923:950	scanning electron microscopy (SEM)	923:956	And the microstructure and composition were detected by scanning electron microscopy (SEM), x-ray diffraction and Fourier transform-infrared spectroscopy.
25464506	2	27	theme	particles	476:484	arg1	powder					439:444	solid powder	433:444	solid powder	433:444	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	27	theme	particles	476:484	arg1	mixture					449:455	a mixture	447:455	a mixture of HA/CS composite particles	447:484	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	4	28	theme	setting	710:716	arg1	time					718:721	the final setting time	700:721	the final setting time	700:721	The results showed that the final setting time was 96.6±5.2 min and the pH value increased from approximately 6.2 to nearly 10 during the setting process and the porosity was 34% at the end.
25464506	4	28	theme	setting	710:716	arg1	min					736:738	96.6±5.2 min	727:738	96.6±5.2 min	727:738	The results showed that the final setting time was 96.6±5.2 min and the pH value increased from approximately 6.2 to nearly 10 during the setting process and the porosity was 34% at the end.
25464506	2	29	theme	HA/CS	460:464	arg1	particles					476:484	HA/CS composite particles	460:484	HA/CS composite particles	460:484	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	0	30	theme	controlled-release	8:25	arg1	system					27:32	A novel controlled-release system	0:32	A novel controlled-release system for antibacterial enzyme lysostaphin delivery	0:78	A novel controlled-release system for antibacterial enzyme lysostaphin delivery using hydroxyapatite/chitosan composite bone cement.
25464506	2	31	theme	Ca	500:501	arg1	OH					503:504	Ca(OH)2	500:506	Ca(OH)2	500:506	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	31	theme	Ca	500:501	arg1	powder					439:444	solid powder	433:444	solid powder	433:444	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	1	32	with	cement	227:232	arg1	delivery					257:264	efficient protein delivery	239:264	efficient protein delivery	239:264	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	0	33	theme	enzyme	52:57	arg1	delivery					71:78	antibacterial enzyme lysostaphin delivery	38:78	antibacterial enzyme lysostaphin delivery	38:78	A novel controlled-release system for antibacterial enzyme lysostaphin delivery using hydroxyapatite/chitosan composite bone cement.
25464506	1	34	theme	composite	343:351	arg1	scaffold					353:360	hydroxyapatite/chitosan (HA/CS) composite scaffold	311:360	hydroxyapatite/chitosan (HA/CS) composite scaffold	311:360	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	6	35	theme	cement	1076:1081	arg1	sample					1083:1088	the cement sample	1072:1088	the cement sample	1072:1088	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	9	36	theme	self-setting	1855:1866	arg1	cement					1891:1896	the lysostaphin-loaded self-setting injectable porous bone cement	1832:1896	the lysostaphin-loaded self-setting injectable porous bone cement	1832:1896	Collectively, all the results suggested that the lysostaphin-loaded self-setting injectable porous bone cement released the protein in a controlled and effective way and the protein activity was well retained during the setting and releasing process.
25464506	3	37	theme	samples	651:657	arg1	parameters					626:635	The setting parameters	614:635	The setting parameters of the cement samples	614:657	The setting parameters of the cement samples were determined.
25464506	1	38	theme	protein	249:255	arg1	delivery					257:264	efficient protein delivery	239:264	efficient protein delivery	239:264	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	10	39	dep	bone	2118:2121	arg1	substitute					2123:2132	substitute	2123:2132	substitute	2123:2132	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	9	40	theme	porous	1879:1884	arg1	cement					1891:1896	the lysostaphin-loaded self-setting injectable porous bone cement	1832:1896	the lysostaphin-loaded self-setting injectable porous bone cement	1832:1896	Collectively, all the results suggested that the lysostaphin-loaded self-setting injectable porous bone cement released the protein in a controlled and effective way and the protein activity was well retained during the setting and releasing process.
25464506	10	41	theme	bone	2118:2121	arg1	system					2157:2162	controlled-release system	2138:2162	controlled-release system for delivery of lysostaphin	2138:2190	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	10	41	theme	bone	2118:2121	arg1	combination					2092:2102	a combination	2090:2102	a combination of artificial bone substitute	2090:2132	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	10	41	theme	bone	2118:2121	arg1	cement					2053:2058	this bone cement	2043:2058	this bone cement	2043:2058	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	2	42	theme	cementitious	400:411	arg1	reactions					413:421	cementitious reactions	400:421	cementitious reactions	400:421	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	8	43	theme	activity	1612:1619	arg1	assay					1621:1625	the antibacterial activity assay	1594:1625	the antibacterial activity assay	1594:1625	Finally the antibacterial activity assay indicated that the loaded lysostaphin had good release ability and strong antibacterial enzymatic activity against methicillin-resistant Staphylococcus aureus.
25464506	7	44	theme	tetrazolium	1420:1430	arg1	assay					1432:1436	the methylthiazol tetrazolium assay	1402:1436	the methylthiazol tetrazolium assay	1402:1436	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	6	45	theme	lysostaphin	1050:1060	arg1	behavior					1038:1045	the release behavior	1026:1045	the release behavior of lysostaphin loaded in the cement sample	1026:1088	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	6	46	theme	in	1218:1219	arg1	experiment					1257:1266	the in vivo Qdot 625 fluorescence tracking experiment	1214:1266	the in vivo Qdot 625 fluorescence tracking experiment	1214:1266	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	1	47	theme	novel	284:288	arg1	method					298:303	a novel setting method	282:303	a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold	282:360	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	2	48	theme	acetic	571:576	arg1	acid					578:581	acetic acid	571:581	acetic acid	571:581	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	7	49	theme	cement	1367:1372	arg1	samples					1374:1380	the cement samples	1363:1380	the cement samples	1363:1380	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	1	50	theme	self-setting	187:198	arg1	cement					227:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement	147:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery	147:264	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	7	51	theme	samples	1374:1380	arg1	biocompatibility					1343:1358	Then the biocompatibility	1334:1358	Then the biocompatibility of the cement samples	1334:1380	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	6	52	theme	cement	1104:1109	arg1	experiment					1119:1128	the in vitro cement extract experiment	1091:1128	the in vitro cement extract experiment	1091:1128	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	9	53	theme	controlled	1924:1933	arg1	way					1949:1951	a controlled and effective way	1922:1951	a controlled and effective way	1922:1951	Collectively, all the results suggested that the lysostaphin-loaded self-setting injectable porous bone cement released the protein in a controlled and effective way and the protein activity was well retained during the setting and releasing process.
25464506	8	54	theme	good	1669:1672	arg1	ability					1682:1688	good release ability	1669:1688	good release ability	1669:1688	Finally the antibacterial activity assay indicated that the loaded lysostaphin had good release ability and strong antibacterial enzymatic activity against methicillin-resistant Staphylococcus aureus.
25464506	1	55	theme	injectable	204:213	arg1	cement					227:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement	147:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery	147:264	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	6	56	theme	in	1095:1096	arg1	experiment					1119:1128	the in vitro cement extract experiment	1091:1128	the in vitro cement extract experiment	1091:1128	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	9	57	theme	effective	1939:1947	arg1	way					1949:1951	a controlled and effective way	1922:1951	a controlled and effective way	1922:1951	Collectively, all the results suggested that the lysostaphin-loaded self-setting injectable porous bone cement released the protein in a controlled and effective way and the protein activity was well retained during the setting and releasing process.
25464506	6	58	dep	in	1095:1096	arg1	vitro					1098:1102	vitro	1098:1102	vitro	1098:1102	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	1	59	theme	bone	222:225	arg1	cement					227:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement	147:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery	147:264	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	8	60	theme	strong	1694:1699	arg1	activity					1725:1732	strong antibacterial enzymatic activity	1694:1732	strong antibacterial enzymatic activity	1694:1732	Finally the antibacterial activity assay indicated that the loaded lysostaphin had good release ability and strong antibacterial enzymatic activity against methicillin-resistant Staphylococcus aureus.
25464506	7	61	theme	subcutaneous	1556:1567	arg1	tissues					1569:1575	subcutaneous tissues	1556:1575	subcutaneous tissues	1556:1575	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	6	62	theme	loaded	1169:1174	arg1	protein					1176:1182	the loaded protein	1165:1182	the loaded protein	1165:1182	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	9	63	theme	protein	1961:1967	arg1	activity					1969:1976	the protein activity	1957:1976	the protein activity	1957:1976	Collectively, all the results suggested that the lysostaphin-loaded self-setting injectable porous bone cement released the protein in a controlled and effective way and the protein activity was well retained during the setting and releasing process.
25464506	6	64	theme	fluorescence	1235:1246	arg1	experiment					1257:1266	the in vivo Qdot 625 fluorescence tracking experiment	1214:1266	the in vivo Qdot 625 fluorescence tracking experiment	1214:1266	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	8	65	theme	enzymatic	1715:1723	arg1	activity					1725:1732	strong antibacterial enzymatic activity	1694:1732	strong antibacterial enzymatic activity	1694:1732	Finally the antibacterial activity assay indicated that the loaded lysostaphin had good release ability and strong antibacterial enzymatic activity against methicillin-resistant Staphylococcus aureus.
25464506	10	66	theme	lysostaphin	2180:2190	arg1	delivery					2168:2175	delivery	2168:2175	delivery of lysostaphin	2168:2190	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	6	67	theme	Qdot	1226:1229	arg1	experiment					1257:1266	the in vivo Qdot 625 fluorescence tracking experiment	1214:1266	the in vivo Qdot 625 fluorescence tracking experiment	1214:1266	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	5	68	theme	Fourier	981:987	arg1	spectroscopy					1008:1019	Fourier transform-infrared spectroscopy	981:1019	Fourier transform-infrared spectroscopy	981:1019	And the microstructure and composition were detected by scanning electron microscopy (SEM), x-ray diffraction and Fourier transform-infrared spectroscopy.
25464506	7	69	theme	MC	1536:1537	arg1	cells					1546:1550	MC 3T3-E1 cells	1536:1550	MC 3T3-E1 cells	1536:1550	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	6	70	theme	free	1324:1327	arg1	one					1329:1331	the free one	1320:1331	the free one	1320:1331	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	1	71	theme	lysostaphin-loaded	149:166	arg1	cement					227:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement	147:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery	147:264	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	7	72	theme	samples	1523:1529	arg1	biocompatibility					1496:1511	good biocompatibility	1491:1511	good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice	1491:1583	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	0	73	theme	composite	110:118	arg1	cement					125:130	hydroxyapatite/chitosan composite bone cement	86:130	hydroxyapatite/chitosan composite bone cement	86:130	A novel controlled-release system for antibacterial enzyme lysostaphin delivery using hydroxyapatite/chitosan composite bone cement.
25464506	1	74	theme	control-released	169:184	arg1	cement					227:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement	147:232	a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery	147:264	In this work, a lysostaphin-loaded, control-released, self-setting and injectable porous bone cement with efficient protein delivery was prepared by a novel setting method using hydroxyapatite/chitosan (HA/CS) composite scaffold.
25464506	10	75	theme	bone	2048:2051	arg1	system					2157:2162	controlled-release system	2138:2162	controlled-release system for delivery of lysostaphin	2138:2190	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	10	75	theme	bone	2048:2051	arg1	combination					2092:2102	a combination	2090:2102	a combination of artificial bone substitute	2090:2132	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	10	75	theme	bone	2048:2051	arg1	cement					2053:2058	this bone cement	2043:2058	this bone cement	2043:2058	Thus this bone cement can be potentially applied as a combination of artificial bone substitute and controlled-release system for delivery of lysostaphin to treat bone defects and infections.
25464506	7	76	theme	good	1491:1494	arg1	biocompatibility					1496:1511	good biocompatibility	1491:1511	good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice	1491:1583	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	6	77	theme	loaded	1284:1289	arg1	protein					1291:1297	the loaded protein	1280:1297	the loaded protein	1280:1297	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	6	78	theme	release	1030:1036	arg1	behavior					1038:1045	the release behavior	1026:1045	the release behavior of lysostaphin loaded in the cement sample	1026:1088	For the release behavior of lysostaphin loaded in the cement sample, the in vitro cement extract experiment indicated that about 94.2±10.9% of the loaded protein was released before day 8 and the in vivo Qdot 625 fluorescence tracking experiment showed that the loaded protein released slower than the free one.
25464506	0	79	theme	novel	2:6	arg1	system					27:32	A novel controlled-release system	0:32	A novel controlled-release system for antibacterial enzyme lysostaphin delivery	0:78	A novel controlled-release system for antibacterial enzyme lysostaphin delivery using hydroxyapatite/chitosan composite bone cement.
25464506	8	80	contain	had	1665:1667	arg1	lysostaphin					1653:1663	the loaded lysostaphin	1642:1663	the loaded lysostaphin	1642:1663	Finally the antibacterial activity assay indicated that the loaded lysostaphin had good release ability and strong antibacterial enzymatic activity against methicillin-resistant Staphylococcus aureus.
25464506	8	80	contain	had	1665:1667	arg2	ability					1682:1688	good release ability	1669:1688	good release ability	1669:1688	Finally the antibacterial activity assay indicated that the loaded lysostaphin had good release ability and strong antibacterial enzymatic activity against methicillin-resistant Staphylococcus aureus.
25464506	8	80	contain	had	1665:1667	arg2	activity					1725:1732	strong antibacterial enzymatic activity	1694:1732	strong antibacterial enzymatic activity	1694:1732	Finally the antibacterial activity assay indicated that the loaded lysostaphin had good release ability and strong antibacterial enzymatic activity against methicillin-resistant Staphylococcus aureus.
25464506	2	81	theme	composite	466:474	arg1	particles					476:484	HA/CS composite particles	460:484	HA/CS composite particles	460:484	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	4	82	theme	final	704:708	arg1	time					718:721	the final setting time	700:721	the final setting time	700:721	The results showed that the final setting time was 96.6±5.2 min and the pH value increased from approximately 6.2 to nearly 10 during the setting process and the porosity was 34% at the end.
25464506	4	82	theme	final	704:708	arg1	min					736:738	96.6±5.2 min	727:738	96.6±5.2 min	727:738	The results showed that the final setting time was 96.6±5.2 min and the pH value increased from approximately 6.2 to nearly 10 during the setting process and the porosity was 34% at the end.
25464506	2	83	contain	containing	547:556	arg2	poloxamer					603:611	poloxamer	603:611	poloxamer	603:611	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	83	contain	containing	547:556	arg2	acid					565:568	citric acid	558:568	citric acid	558:568	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	83	contain	containing	547:556	arg2	acid					578:581	acetic acid	571:581	acetic acid	571:581	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	83	contain	containing	547:556	arg2	NaH2PO4					584:590	NaH2PO4	584:590	NaH2PO4	584:590	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	83	contain	containing	547:556	arg2	CaCl2					593:597	CaCl2	593:597	CaCl2	593:597	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	2	83	contain	containing	547:556	arg1	liquid					540:545	liquid	540:545	liquid	540:545	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	3	84	theme	setting	618:624	arg1	parameters					626:635	The setting parameters	614:635	The setting parameters of the cement samples	614:657	The setting parameters of the cement samples were determined.
25464506	0	85	theme	antibacterial	38:50	arg1	delivery					71:78	antibacterial enzyme lysostaphin delivery	38:78	antibacterial enzyme lysostaphin delivery	38:78	A novel controlled-release system for antibacterial enzyme lysostaphin delivery using hydroxyapatite/chitosan composite bone cement.
25464506	0	86	theme	lysostaphin	59:69	arg1	delivery					71:78	antibacterial enzyme lysostaphin delivery	38:78	antibacterial enzyme lysostaphin delivery	38:78	A novel controlled-release system for antibacterial enzyme lysostaphin delivery using hydroxyapatite/chitosan composite bone cement.
25464506	5	87	theme	x-ray	959:963	arg1	diffraction					965:975	x-ray diffraction	959:975	x-ray diffraction	959:975	And the microstructure and composition were detected by scanning electron microscopy (SEM), x-ray diffraction and Fourier transform-infrared spectroscopy.
25464506	9	88	theme	lysostaphin-loaded	1836:1853	arg1	cement					1891:1896	the lysostaphin-loaded self-setting injectable porous bone cement	1832:1896	the lysostaphin-loaded self-setting injectable porous bone cement	1832:1896	Collectively, all the results suggested that the lysostaphin-loaded self-setting injectable porous bone cement released the protein in a controlled and effective way and the protein activity was well retained during the setting and releasing process.
25464506	3	89	theme	cement	644:649	arg1	samples					651:657	the cement samples	640:657	the cement samples	640:657	The setting parameters of the cement samples were determined.
25464506	2	90	theme	cement	367:372	arg1	samples					374:380	The cement samples	363:380	The cement samples	363:380	The cement samples were made through cementitious reactions by mixing solid powder, a mixture of HA/CS composite particles, lysostaphin, Ca(OH)2, CaCO3 and NaHCO3, with setting liquid containing citric acid, acetic acid, NaH2PO4, CaCl2 and poloxamer.
25464506	8	91	theme	antibacterial	1598:1610	arg1	assay					1621:1625	the antibacterial activity assay	1594:1625	the antibacterial activity assay	1594:1625	Finally the antibacterial activity assay indicated that the loaded lysostaphin had good release ability and strong antibacterial enzymatic activity against methicillin-resistant Staphylococcus aureus.
25464506	7	92	theme	hematoxylin-eosin	1447:1463	arg1	staining					1465:1472	hematoxylin-eosin staining	1447:1472	hematoxylin-eosin staining	1447:1472	Then the biocompatibility of the cement samples was evaluated using the methylthiazol tetrazolium assay, SEM and hematoxylin-eosin staining, which suggested good biocompatibility of cement samples with MC 3T3-E1 cells and subcutaneous tissues of mice.
25464506	5	93	theme	electron	932:939	arg1	SEM					953:955	SEM	953:955	SEM	953:955	And the microstructure and composition were detected by scanning electron microscopy (SEM), x-ray diffraction and Fourier transform-infrared spectroscopy.
25464506	5	93	theme	electron	932:939	arg1	microscopy					941:950	scanning electron microscopy	923:950	scanning electron microscopy (SEM)	923:956	And the microstructure and composition were detected by scanning electron microscopy (SEM), x-ray diffraction and Fourier transform-infrared spectroscopy.
25464506	9	94	theme	injectable	1868:1877	arg1	cement					1891:1896	the lysostaphin-loaded self-setting injectable porous bone cement	1832:1896	the lysostaphin-loaded self-setting injectable porous bone cement	1832:1896	Collectively, all the results suggested that the lysostaphin-loaded self-setting injectable porous bone cement released the protein in a controlled and effective way and the protein activity was well retained during the setting and releasing process.
25464506	4	95	theme	pH	748:749	arg1	value					751:755	the pH value	744:755	the pH value	744:755	The results showed that the final setting time was 96.6±5.2 min and the pH value increased from approximately 6.2 to nearly 10 during the setting process and the porosity was 34% at the end.
25464506	9	96	theme	bone	1886:1889	arg1	cement					1891:1896	the lysostaphin-loaded self-setting injectable porous bone cement	1832:1896	the lysostaphin-loaded self-setting injectable porous bone cement	1832:1896	Collectively, all the results suggested that the lysostaphin-loaded self-setting injectable porous bone cement released the protein in a controlled and effective way and the protein activity was well retained during the setting and releasing process.
26855011	6	0	theme	recombinant	1026:1036	arg1	protein					1038:1044	the recombinant protein	1022:1044	the recombinant protein	1022:1044	From the amino acid sequence, immunoreactivity, and properties of the recombinant protein, Intl-3 was considered the intestinal lectin identified by the anti-XEEL antibody.
26855011	2	1	theme	family	311:316	arg1	lectins					318:324	six Intl family lectins	302:324	six Intl family lectins	302:324	Although six Intl family lectins have so far been reported in Xenopus laevis, none have been identified in the intestine.
26855011	11	2	theme	digestive	1895:1903	arg1	tract					1905:1909	the digestive tract	1891:1909	the digestive tract	1891:1909	The results of this study suggest that Xenopus Intl-3 is involved in the innate immune protection of the digestive tract against bacterial infections.
26855011	1	3	theme	intelectin	104:113	arg1	lectins					153:159	secretory lectins	143:159	secretory lectins in chordates that serve multiple functions, including innate immunity	143:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	1	3	theme	intelectin	104:113	arg1	family					122:127	The intelectin (Intl) family	100:127	The intelectin (Intl) family	100:127	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	1	3	theme	intelectin	104:113	arg1	group					134:138	a group	132:138	a group of secretory lectins in chordates that serve multiple functions, including innate immunity	132:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	6	4	theme	anti-XEEL	1109:1117	arg1	antibody					1119:1126	the anti-XEEL antibody	1105:1126	the anti-XEEL antibody	1105:1126	From the amino acid sequence, immunoreactivity, and properties of the recombinant protein, Intl-3 was considered the intestinal lectin identified by the anti-XEEL antibody.
26855011	4	5	from	chromatography	598:611	arg1	column					638:643	a galactose-Sepharose column	616:643	a galactose-Sepharose column	616:643	The proteins were purified by affinity chromatography on a galactose-Sepharose column and found to be oligomers consisting of N-glycosylated 39 kDa and 40.5 kDa subunit peptides.
26855011	1	6	theme	secretory	143:151	arg1	lectins					153:159	secretory lectins	143:159	secretory lectins in chordates that serve multiple functions, including innate immunity	143:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	5	7	theme	novel	831:835	arg1	Intls					837:841	two novel Intls	827:841	two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins	827:953	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	3	8	theme	cross-reactive	516:529	arg1	proteins					531:538	cross-reactive proteins	516:538	cross-reactive proteins	516:538	Using a monoclonal antibody to the Xenopus embryonic epidermal lectin (XEEL or Intl-1), I identified cross-reactive proteins in the intestines.
26855011	9	9	theme	Intl-3	1476:1481	arg1	proteins					1515:1522	Intl-3 and XCL-1 (or 35K serum lectin) proteins	1476:1522	Intl-3 and XCL-1 (or 35K serum lectin) proteins	1476:1522	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	3	10	theme	Xenopus	450:456	arg1	lectin					478:483	the Xenopus embryonic epidermal lectin	446:483	the Xenopus embryonic epidermal lectin (XEEL or Intl-1)	446:500	Using a monoclonal antibody to the Xenopus embryonic epidermal lectin (XEEL or Intl-1), I identified cross-reactive proteins in the intestines.
26855011	0	11	from	tract	75:79	arg1	characterization					19:34	characterization	19:34	characterization	19:34	Identification and characterization of a novel intelectin in the digestive tract of Xenopus laevis.
26855011	0	11	from	tract	75:79	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and characterization of a novel intelectin in the digestive tract of Xenopus laevis.
26855011	6	12	theme	amino	965:969	arg1	sequence					976:983	the amino acid sequence	961:983	the amino acid sequence	961:983	From the amino acid sequence, immunoreactivity, and properties of the recombinant protein, Intl-3 was considered the intestinal lectin identified by the anti-XEEL antibody.
26855011	1	13	theme	multiple	185:192	arg1	functions					194:202	multiple functions	185:202	multiple functions	185:202	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	1	13	theme	multiple	185:192	arg1	immunity					222:229	innate immunity	215:229	innate immunity	215:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	10	14	theme	Immunohistochemical	1661:1679	arg1	analyses					1681:1688	Immunohistochemical analyses	1661:1688	Immunohistochemical analyses	1661:1688	Immunohistochemical analyses revealed accumulation of the Intl-3 protein in mucus secretory granules of intestinal goblet cells.
26855011	1	15	from	chordates	164:172	arg1	lectins					153:159	secretory lectins	143:159	secretory lectins in chordates that serve multiple functions, including innate immunity	143:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	1	15	from	chordates	164:172	arg1	family					122:127	The intelectin (Intl) family	100:127	The intelectin (Intl) family	100:127	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	1	15	from	chordates	164:172	arg1	group					134:138	a group	132:138	a group of secretory lectins in chordates that serve multiple functions, including innate immunity	132:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	11	16	theme	innate	1863:1868	arg1	protection					1877:1886	the innate immune protection	1859:1886	the innate immune protection of the digestive tract against bacterial infections	1859:1938	The results of this study suggest that Xenopus Intl-3 is involved in the innate immune protection of the digestive tract against bacterial infections.
26855011	10	17	theme	Intl-3	1719:1724	arg1	protein					1726:1732	the Intl-3 protein	1715:1732	the Intl-3 protein	1715:1732	Immunohistochemical analyses revealed accumulation of the Intl-3 protein in mucus secretory granules of intestinal goblet cells.
26855011	1	18	from	lectins	153:159	arg1	chordates					164:172	chordates	164:172	chordates that serve multiple functions, including innate immunity	164:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	9	19	theme	Intraperitoneal	1395:1409	arg1	injection					1411:1419	Intraperitoneal injection	1395:1419	Intraperitoneal injection of LPS	1395:1426	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	9	20	theme	LPS	1424:1426	arg1	injection					1411:1419	Intraperitoneal injection	1395:1419	Intraperitoneal injection of LPS	1395:1426	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	0	21	from	characterization	19:34	arg1	tract					75:79	the digestive tract	61:79	the digestive tract of Xenopus laevis	61:97	Identification and characterization of a novel intelectin in the digestive tract of Xenopus laevis.
26855011	0	22	from	Identification	0:13	arg1	tract					75:79	the digestive tract	61:79	the digestive tract of Xenopus laevis	61:97	Identification and characterization of a novel intelectin in the digestive tract of Xenopus laevis.
26855011	4	23	theme	subunit	720:726	arg1	peptides					728:735	N-glycosylated 39 kDa and 40.5 kDa subunit peptides	685:735	N-glycosylated 39 kDa and 40.5 kDa subunit peptides	685:735	The proteins were purified by affinity chromatography on a galactose-Sepharose column and found to be oligomers consisting of N-glycosylated 39 kDa and 40.5 kDa subunit peptides.
26855011	9	24	theme	XCL-1	1487:1491	arg1	proteins					1515:1522	Intl-3 and XCL-1 (or 35K serum lectin) proteins	1476:1522	Intl-3 and XCL-1 (or 35K serum lectin) proteins	1476:1522	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	0	25	theme	Xenopus	84:90	arg1	laevis					92:97	Xenopus laevis	84:97	Xenopus laevis	84:97	Identification and characterization of a novel intelectin in the digestive tract of Xenopus laevis.
26855011	0	26	from	intelectin	47:56	arg1	tract					75:79	the digestive tract	61:79	the digestive tract of Xenopus laevis	61:97	Identification and characterization of a novel intelectin in the digestive tract of Xenopus laevis.
26855011	7	27	theme	purified	1133:1140	arg1	protein					1149:1155	The purified Intl-3 protein	1129:1155	The purified Intl-3 protein	1129:1155	The purified Intl-3 protein could potentially bind to Escherichia coli and its lipopolysaccharides (LPS), and to Staphylococcus aureus and its peptidoglycans, depending on Ca(2+).
26855011	0	28	theme	novel	41:45	arg1	intelectin					47:56	a novel intelectin	39:56	a novel intelectin in the digestive tract of Xenopus laevis	39:97	Identification and characterization of a novel intelectin in the digestive tract of Xenopus laevis.
26855011	5	29	theme	known	920:924	arg1	proteins					946:953	known Xenopus Intl family proteins	920:953	known Xenopus Intl family proteins	920:953	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	10	30	from	accumulation	1699:1710	arg1	granules					1753:1760	mucus secretory granules	1737:1760	mucus secretory granules of intestinal goblet cells	1737:1787	Immunohistochemical analyses revealed accumulation of the Intl-3 protein in mucus secretory granules of intestinal goblet cells.
26855011	4	31	theme	N-glycosylated	685:698	arg1	peptides					728:735	N-glycosylated 39 kDa and 40.5 kDa subunit peptides	685:735	N-glycosylated 39 kDa and 40.5 kDa subunit peptides	685:735	The proteins were purified by affinity chromatography on a galactose-Sepharose column and found to be oligomers consisting of N-glycosylated 39 kDa and 40.5 kDa subunit peptides.
26855011	5	32	theme	59-79	886:890	arg1	%					891:891	%	891:891	%	891:891	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	5	33	dep	Intls	837:841	arg1	designated					844:853	designated	844:853	designated Intl-3 and Intl-4	844:871	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	5	34	theme	amino	893:897	arg1	identities					904:913	59-79% amino acid identities	886:913	59-79% amino acid identities	886:913	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	10	35	theme	secretory	1743:1751	arg1	granules					1753:1760	mucus secretory granules	1737:1760	mucus secretory granules of intestinal goblet cells	1737:1787	Immunohistochemical analyses revealed accumulation of the Intl-3 protein in mucus secretory granules of intestinal goblet cells.
26855011	5	36	theme	amino	749:753	arg1	sequencing					760:769	N-terminal amino acid sequencing	738:769	N-terminal amino acid sequencing	738:769	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	6	37	theme	acid	971:974	arg1	sequence					976:983	the amino acid sequence	961:983	the amino acid sequence	961:983	From the amino acid sequence, immunoreactivity, and properties of the recombinant protein, Intl-3 was considered the intestinal lectin identified by the anti-XEEL antibody.
26855011	2	38	theme	Intl	306:309	arg1	lectins					318:324	six Intl family lectins	302:324	six Intl family lectins	302:324	Although six Intl family lectins have so far been reported in Xenopus laevis, none have been identified in the intestine.
26855011	0	39	theme	intelectin	47:56	arg1	characterization					19:34	characterization	19:34	characterization	19:34	Identification and characterization of a novel intelectin in the digestive tract of Xenopus laevis.
26855011	0	39	theme	intelectin	47:56	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and characterization of a novel intelectin in the digestive tract of Xenopus laevis.
26855011	1	40	theme	carbohydrate	272:283	arg1	chains					285:290	carbohydrate chains	272:290	carbohydrate chains	272:290	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	9	41	from	tissues	1622:1628	arg1	detectable					1577:1586	detectable	1577:1586	detectable	1577:1586	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	9	42	theme	serum	1501:1505	arg1	Intl-3					1476:1481	Intl-3	1476:1481	Intl-3	1476:1481	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	9	42	theme	serum	1501:1505	arg1	lectin					1507:1512	35K serum lectin	1497:1512	35K serum lectin	1497:1512	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	10	43	theme	goblet	1776:1781	arg1	cells					1783:1787	intestinal goblet cells	1765:1787	intestinal goblet cells	1765:1787	Immunohistochemical analyses revealed accumulation of the Intl-3 protein in mucus secretory granules of intestinal goblet cells.
26855011	5	44	theme	Intl	934:937	arg1	proteins					946:953	known Xenopus Intl family proteins	920:953	known Xenopus Intl family proteins	920:953	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	11	45	theme	study	1810:1814	arg1	results					1794:1800	The results	1790:1800	The results of this study	1790:1814	The results of this study suggest that Xenopus Intl-3 is involved in the innate immune protection of the digestive tract against bacterial infections.
26855011	8	46	theme	E. coli	1354:1360	arg1	cells					1362:1366	E. coli cells	1354:1366	E. coli cells	1354:1366	In addition, the Intl-3 protein agglutinated E. coli cells in the presence of Ca(2+).
26855011	3	47	theme	embryonic	458:466	arg1	lectin					478:483	the Xenopus embryonic epidermal lectin	446:483	the Xenopus embryonic epidermal lectin (XEEL or Intl-1)	446:500	Using a monoclonal antibody to the Xenopus embryonic epidermal lectin (XEEL or Intl-1), I identified cross-reactive proteins in the intestines.
26855011	6	48	theme	protein	1038:1044	arg1	immunoreactivity					986:1001	immunoreactivity	986:1001	immunoreactivity	986:1001	From the amino acid sequence, immunoreactivity, and properties of the recombinant protein, Intl-3 was considered the intestinal lectin identified by the anti-XEEL antibody.
26855011	6	48	theme	protein	1038:1044	arg1	properties					1008:1017	properties	1008:1017	properties of the recombinant protein	1008:1044	From the amino acid sequence, immunoreactivity, and properties of the recombinant protein, Intl-3 was considered the intestinal lectin identified by the anti-XEEL antibody.
26855011	6	48	theme	protein	1038:1044	arg1	sequence					976:983	the amino acid sequence	961:983	the amino acid sequence	961:983	From the amino acid sequence, immunoreactivity, and properties of the recombinant protein, Intl-3 was considered the intestinal lectin identified by the anti-XEEL antibody.
26855011	4	49	theme	affinity	589:596	arg1	chromatography					598:611	affinity chromatography	589:611	affinity chromatography on a galactose-Sepharose column	589:643	The proteins were purified by affinity chromatography on a galactose-Sepharose column and found to be oligomers consisting of N-glycosylated 39 kDa and 40.5 kDa subunit peptides.
26855011	11	50	theme	bacterial	1919:1927	arg1	infections					1929:1938	bacterial infections	1919:1938	bacterial infections	1919:1938	The results of this study suggest that Xenopus Intl-3 is involved in the innate immune protection of the digestive tract against bacterial infections.
26855011	1	51	theme	lectins	153:159	arg1	lectins					153:159	secretory lectins	143:159	secretory lectins in chordates that serve multiple functions, including innate immunity	143:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	1	51	theme	lectins	153:159	arg1	family					122:127	The intelectin (Intl) family	100:127	The intelectin (Intl) family	100:127	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	1	51	theme	lectins	153:159	arg1	group					134:138	a group	132:138	a group of secretory lectins in chordates that serve multiple functions, including innate immunity	132:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	11	52	theme	tract	1905:1909	arg1	protection					1877:1886	the innate immune protection	1859:1886	the innate immune protection of the digestive tract against bacterial infections	1859:1938	The results of this study suggest that Xenopus Intl-3 is involved in the innate immune protection of the digestive tract against bacterial infections.
26855011	6	53	theme	intestinal	1073:1082	arg1	lectin					1084:1089	the intestinal lectin	1069:1089	the intestinal lectin identified by the anti-XEEL antibody	1069:1126	From the amino acid sequence, immunoreactivity, and properties of the recombinant protein, Intl-3 was considered the intestinal lectin identified by the anti-XEEL antibody.
26855011	5	54	theme	cDNA	802:805	arg1	cloning					807:813	cDNA cloning	802:813	cDNA cloning	802:813	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	7	55	theme	Intl-3	1142:1147	arg1	protein					1149:1155	The purified Intl-3 protein	1129:1155	The purified Intl-3 protein	1129:1155	The purified Intl-3 protein could potentially bind to Escherichia coli and its lipopolysaccharides (LPS), and to Staphylococcus aureus and its peptidoglycans, depending on Ca(2+).
26855011	11	56	theme	Xenopus	1829:1835	arg1	Intl-3					1837:1842	Xenopus Intl-3	1829:1842	Xenopus Intl-3	1829:1842	The results of this study suggest that Xenopus Intl-3 is involved in the innate immune protection of the digestive tract against bacterial infections.
26855011	3	57	dep	lectin	478:483	arg1	XEEL					486:489	XEEL	486:489	XEEL	486:489	Using a monoclonal antibody to the Xenopus embryonic epidermal lectin (XEEL or Intl-1), I identified cross-reactive proteins in the intestines.
26855011	3	57	dep	lectin	478:483	arg1	Intl-1					494:499	Intl-1	494:499	Intl-1	494:499	Using a monoclonal antibody to the Xenopus embryonic epidermal lectin (XEEL or Intl-1), I identified cross-reactive proteins in the intestines.
26855011	8	58	theme	Ca	1387:1388	arg1	presence					1375:1382	the presence	1371:1382	the presence of Ca(2+)	1371:1392	In addition, the Intl-3 protein agglutinated E. coli cells in the presence of Ca(2+).
26855011	10	59	theme	protein	1726:1732	arg1	accumulation					1699:1710	accumulation	1699:1710	accumulation of the Intl-3 protein in mucus secretory granules of intestinal goblet cells	1699:1787	Immunohistochemical analyses revealed accumulation of the Intl-3 protein in mucus secretory granules of intestinal goblet cells.
26855011	1	60	theme	Intl	116:119	arg1	lectins					153:159	secretory lectins	143:159	secretory lectins in chordates that serve multiple functions, including innate immunity	143:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	1	60	theme	Intl	116:119	arg1	family					122:127	The intelectin (Intl) family	100:127	The intelectin (Intl) family	100:127	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	1	60	theme	Intl	116:119	arg1	group					134:138	a group	132:138	a group of secretory lectins in chordates that serve multiple functions, including innate immunity	132:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	9	61	from	sera	1603:1606	arg1	detectable					1577:1586	detectable	1577:1586	detectable	1577:1586	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	5	62	theme	acid	899:902	arg1	identities					904:913	59-79% amino acid identities	886:913	59-79% amino acid identities	886:913	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	0	63	theme	digestive	65:73	arg1	tract					75:79	the digestive tract	61:79	the digestive tract of Xenopus laevis	61:97	Identification and characterization of a novel intelectin in the digestive tract of Xenopus laevis.
26855011	11	64	theme	immune	1870:1875	arg1	protection					1877:1886	the innate immune protection	1859:1886	the innate immune protection of the digestive tract against bacterial infections	1859:1938	The results of this study suggest that Xenopus Intl-3 is involved in the innate immune protection of the digestive tract against bacterial infections.
26855011	9	65	theme	other	1616:1620	arg1	tissues					1622:1628	the other tissues	1612:1628	the other tissues regardless of LPS stimulation	1612:1658	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	4	66	theme	galactose-Sepharose	618:636	arg1	column					638:643	a galactose-Sepharose column	616:643	a galactose-Sepharose column	616:643	The proteins were purified by affinity chromatography on a galactose-Sepharose column and found to be oligomers consisting of N-glycosylated 39 kDa and 40.5 kDa subunit peptides.
26855011	4	67	gly	N-glycosylated	685:698	arg1	peptides					728:735	N-glycosylated 39 kDa and 40.5 kDa subunit peptides	685:735	N-glycosylated 39 kDa and 40.5 kDa subunit peptides	685:735	The proteins were purified by affinity chromatography on a galactose-Sepharose column and found to be oligomers consisting of N-glycosylated 39 kDa and 40.5 kDa subunit peptides.
26855011	9	68	from	detectable	1577:1586	arg1	tissues					1622:1628	the other tissues	1612:1628	the other tissues regardless of LPS stimulation	1612:1658	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	9	68	from	detectable	1577:1586	arg1	sera					1603:1606	the sera	1599:1606	the sera	1599:1606	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	0	69	theme	laevis	92:97	arg1	tract					75:79	the digestive tract	61:79	the digestive tract of Xenopus laevis	61:97	Identification and characterization of a novel intelectin in the digestive tract of Xenopus laevis.
26855011	1	70	from	group	134:138	arg1	chordates					164:172	chordates	164:172	chordates that serve multiple functions, including innate immunity	164:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	9	71	theme	intestinal	1442:1451	arg1	contents					1464:1471	the intestinal and rectal contents	1438:1471	the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins	1438:1522	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	9	72	theme	LPS	1644:1646	arg1	stimulation					1648:1658	LPS stimulation	1644:1658	LPS stimulation	1644:1658	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	4	73	theme	40.5 kDa	711:718	arg1	peptides					728:735	N-glycosylated 39 kDa and 40.5 kDa subunit peptides	685:735	N-glycosylated 39 kDa and 40.5 kDa subunit peptides	685:735	The proteins were purified by affinity chromatography on a galactose-Sepharose column and found to be oligomers consisting of N-glycosylated 39 kDa and 40.5 kDa subunit peptides.
26855011	9	74	theme	rectal	1457:1462	arg1	contents					1464:1471	the intestinal and rectal contents	1438:1471	the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins	1438:1522	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	5	75	theme	peptides	780:787	arg1	sequencing					760:769	N-terminal amino acid sequencing	738:769	N-terminal amino acid sequencing	738:769	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	5	76	theme	family	939:944	arg1	proteins					946:953	known Xenopus Intl family proteins	920:953	known Xenopus Intl family proteins	920:953	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	5	77	theme	%	891:891	arg1	identities					904:913	59-79% amino acid identities	886:913	59-79% amino acid identities	886:913	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	4	78	theme	39 kDa	700:705	arg1	peptides					728:735	N-glycosylated 39 kDa and 40.5 kDa subunit peptides	685:735	N-glycosylated 39 kDa and 40.5 kDa subunit peptides	685:735	The proteins were purified by affinity chromatography on a galactose-Sepharose column and found to be oligomers consisting of N-glycosylated 39 kDa and 40.5 kDa subunit peptides.
26855011	5	79	theme	N-terminal	738:747	arg1	sequencing					760:769	N-terminal amino acid sequencing	738:769	N-terminal amino acid sequencing	738:769	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	1	80	theme	-dependent	246:255	arg1	recognition					257:267	Ca(2+)-dependent recognition	240:267	Ca(2+)-dependent recognition of carbohydrate chains	240:290	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	10	81	theme	mucus	1737:1741	arg1	granules					1753:1760	mucus secretory granules	1737:1760	mucus secretory granules of intestinal goblet cells	1737:1787	Immunohistochemical analyses revealed accumulation of the Intl-3 protein in mucus secretory granules of intestinal goblet cells.
26855011	2	82	theme	Xenopus	355:361	arg1	laevis					363:368	Xenopus laevis	355:368	Xenopus laevis	355:368	Although six Intl family lectins have so far been reported in Xenopus laevis, none have been identified in the intestine.
26855011	5	83	theme	acid	755:758	arg1	sequencing					760:769	N-terminal amino acid sequencing	738:769	N-terminal amino acid sequencing	738:769	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	9	84	theme	35K	1497:1499	arg1	Intl-3					1476:1481	Intl-3	1476:1481	Intl-3	1476:1481	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	9	84	theme	35K	1497:1499	arg1	lectin					1507:1512	35K serum lectin	1497:1512	35K serum lectin	1497:1512	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	10	85	theme	cells	1783:1787	arg1	granules					1753:1760	mucus secretory granules	1737:1760	mucus secretory granules of intestinal goblet cells	1737:1787	Immunohistochemical analyses revealed accumulation of the Intl-3 protein in mucus secretory granules of intestinal goblet cells.
26855011	1	86	theme	chains	285:290	arg1	recognition					257:267	Ca(2+)-dependent recognition	240:267	Ca(2+)-dependent recognition of carbohydrate chains	240:290	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
26855011	5	87	theme	Xenopus	926:932	arg1	proteins					946:953	known Xenopus Intl family proteins	920:953	known Xenopus Intl family proteins	920:953	N-terminal amino acid sequencing of these peptides, followed by cDNA cloning, identified two novel Intls (designated Intl-3 and Intl-4) that showed 59-79% amino acid identities with known Xenopus Intl family proteins.
26855011	10	88	theme	intestinal	1765:1774	arg1	cells					1783:1787	intestinal goblet cells	1765:1787	intestinal goblet cells	1765:1787	Immunohistochemical analyses revealed accumulation of the Intl-3 protein in mucus secretory granules of intestinal goblet cells.
26855011	8	89	theme	Intl-3	1326:1331	arg1	protein					1333:1339	the Intl-3 protein	1322:1339	the Intl-3 protein	1322:1339	In addition, the Intl-3 protein agglutinated E. coli cells in the presence of Ca(2+).
26855011	3	90	theme	monoclonal	423:432	arg1	antibody					434:441	a monoclonal antibody	421:441	a monoclonal antibody to the Xenopus embryonic epidermal lectin (XEEL or Intl-1)	421:500	Using a monoclonal antibody to the Xenopus embryonic epidermal lectin (XEEL or Intl-1), I identified cross-reactive proteins in the intestines.
26855011	9	91	theme	proteins	1515:1522	arg1	contents					1464:1471	the intestinal and rectal contents	1438:1471	the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins	1438:1522	Intraperitoneal injection of LPS increased the intestinal and rectal contents of Intl-3 and XCL-1 (or 35K serum lectin) proteins within three days; however, unlike XCL-1, Intl-3 was detectable in neither the sera nor the other tissues regardless of LPS stimulation.
26855011	3	92	theme	epidermal	468:476	arg1	lectin					478:483	the Xenopus embryonic epidermal lectin	446:483	the Xenopus embryonic epidermal lectin (XEEL or Intl-1)	446:500	Using a monoclonal antibody to the Xenopus embryonic epidermal lectin (XEEL or Intl-1), I identified cross-reactive proteins in the intestines.
26855011	1	93	theme	innate	215:220	arg1	immunity					222:229	innate immunity	215:229	innate immunity	215:229	The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains.
25435398	9	0	theme	microwave	1482:1490	arg1	extraction					1501:1510	conventional and microwave assisted extraction	1465:1510	conventional and microwave assisted extraction	1465:1510	Pectin was extracted by conventional and microwave assisted extraction.
25435398	1	1	dep	in	387:388	arg1	situ					390:393	situ	390:393	situ	390:393	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	8	2	from	increase	1411:1418	arg1	yield					1434:1438	TPC yield	1430:1438	TPC yield	1430:1438	Compared with the conventional extraction (CE), the UAE gave an increase of 30% in TPC yield.
25435398	1	3	theme	"	394:394	arg1	solvent					433:439	solvent	433:439	solvent	433:439	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	1	3	theme	"	394:394	arg1	water					396:400	"in situ" water	386:400	"in situ" water which was recycled and used as solvent	386:439	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	10	4	theme	%	1557:1557	arg1	yield					1544:1548	a maximal yield	1534:1548	a maximal yield of 24.2% for microwave power of 500 W	1534:1586	This technique gives a maximal yield of 24.2% for microwave power of 500 W in only 3 min whereas conventional extraction gives 18.32% in 120 min.
25435398	1	5	from	peel	284:287	arg1	extraction					220:229	extraction	220:229	extraction of essential oil, polyphenols and pectin from orange peel	220:287	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	4	6	theme	MHG	773:775	arg1	extraction					777:786	MHG extraction	773:786	MHG extraction	773:786	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	10	7	theme	W	1586:1586	arg1	power					1573:1577	microwave power	1563:1577	microwave power of 500 W	1563:1586	This technique gives a maximal yield of 24.2% for microwave power of 500 W in only 3 min whereas conventional extraction gives 18.32% in 120 min.
25435398	1	8	theme	oil	244:246	arg1	extraction					220:229	extraction	220:229	extraction of essential oil, polyphenols and pectin from orange peel	220:287	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	7	9	theme	dm	1343:1344	arg1	yield					1317:1321	a polyphenol yield	1304:1321	a polyphenol yield of 50.02 mgGA/100 g dm	1304:1344	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	4	10	theme	residual	734:741	arg1	water					743:747	residual water	734:747	residual water of plant obtained after MHG extraction	734:786	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	4	10	theme	residual	734:741	arg1	solvent					800:806	solvent	800:806	solvent	800:806	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	3	11	theme	±	644:644	arg1	%					650:650	4.22 ± 0.03%	639:650	4.22 ± 0.03%	639:650	No significant changes in yield were noticed: 4.22 ± 0.03% and 4.16 ± 0.05% for MHG and SD, respectively.
25435398	2	12	theme	distillation	563:574	arg1	SD					588:589	SD	588:589	SD	588:589	The essential oil extraction performed by Microwave Hydrodiffusion and Gravity (MHG) was optimized and compared to steam distillation extraction (SD).
25435398	2	12	theme	distillation	563:574	arg1	extraction					576:585	steam distillation extraction	557:585	steam distillation extraction (SD)	557:590	The essential oil extraction performed by Microwave Hydrodiffusion and Gravity (MHG) was optimized and compared to steam distillation extraction (SD).
25435398	6	13	theme	ultrasound-assisted	1128:1146	arg1	UAE					1160:1162	UAE	1160:1162	UAE	1160:1162	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	6	13	theme	ultrasound-assisted	1128:1146	arg1	extraction					1148:1157	the ultrasound-assisted extraction	1124:1157	the ultrasound-assisted extraction (UAE)	1124:1163	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	4	14	theme	plant	752:756	arg1	water					743:747	residual water	734:747	residual water of plant obtained after MHG extraction	734:786	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	4	14	theme	plant	752:756	arg1	solvent					800:806	solvent	800:806	solvent	800:806	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	0	15	theme	extraction	93:102	arg1	processes					104:112	solvent free extraction processes	80:112	solvent free extraction processes	80:112	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.
25435398	11	16	theme	ultrasound	1685:1694	arg1	Combination					1659:1669	Combination	1659:1669	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels	1659:1743	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	6	17	theme	variables	1111:1119	arg1	influence					1090:1098	the influence	1086:1098	the influence of process variables on the ultrasound-assisted extraction (UAE)	1086:1163	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	10	18	theme	conventional	1610:1621	arg1	extraction					1623:1632	conventional extraction	1610:1632	conventional extraction	1610:1632	This technique gives a maximal yield of 24.2% for microwave power of 500 W in only 3 min whereas conventional extraction gives 18.32% in 120 min.
25435398	7	19	theme	temperature	1254:1264	arg1	conditions					1219:1228	the optimized conditions	1205:1228	the optimized conditions of ultrasound power and temperature	1205:1264	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	7	19	theme	temperature	1254:1264	arg1	W/cm					1277:1280	0.956 W/cm	1271:1280	0.956 W/cm(2)	1271:1283	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	1	20	theme	in	387:388	arg1	solvent					433:439	solvent	433:439	solvent	433:439	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	1	20	theme	in	387:388	arg1	water					396:400	"in situ" water	386:400	"in situ" water which was recycled and used as solvent	386:439	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	11	21	theme	values	1775:1780	arg1	compounds					1782:1790	high added values compounds	1764:1790	high added values compounds in shorter time	1764:1806	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	2	22	theme	essential	446:454	arg1	extraction					460:469	The essential oil extraction	442:469	The essential oil extraction performed by Microwave Hydrodiffusion and Gravity (MHG)	442:525	The essential oil extraction performed by Microwave Hydrodiffusion and Gravity (MHG) was optimized and compared to steam distillation extraction (SD).
25435398	7	23	theme	power	1244:1248	arg1	conditions					1219:1228	the optimized conditions	1205:1228	the optimized conditions of ultrasound power and temperature	1205:1264	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	7	23	theme	power	1244:1248	arg1	W/cm					1277:1280	0.956 W/cm	1271:1280	0.956 W/cm(2)	1271:1283	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	11	24	theme	high	1764:1767	arg1	compounds					1782:1790	high added values compounds	1764:1790	high added values compounds in shorter time	1764:1806	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	8	25	theme	conventional	1365:1376	arg1	CE					1390:1391	CE	1390:1391	CE	1390:1391	Compared with the conventional extraction (CE), the UAE gave an increase of 30% in TPC yield.
25435398	8	25	theme	conventional	1365:1376	arg1	extraction					1378:1387	the conventional extraction	1361:1387	the conventional extraction (CE)	1361:1392	Compared with the conventional extraction (CE), the UAE gave an increase of 30% in TPC yield.
25435398	6	26	theme	central	1017:1023	arg1	CCD					1044:1046	CCD	1044:1046	CCD	1044:1046	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	6	26	theme	central	1017:1023	arg1	designs					1035:1041	central composite designs	1017:1041	central composite designs (CCD) approach	1017:1056	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	6	27	from	influence	1090:1098	arg1	UAE					1160:1162	UAE	1160:1162	UAE	1160:1162	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	6	27	from	influence	1090:1098	arg1	extraction					1148:1157	the ultrasound-assisted extraction	1124:1157	the ultrasound-assisted extraction (UAE)	1124:1163	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	4	28	theme	MHG	851:853	arg1	residues					855:862	MHG residues	851:862	MHG residues	851:862	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	11	29	theme	in	1714:1715	arg1	water					1723:1727	the recycled "in situ" water	1700:1727	the recycled "in situ" water of citrus peels	1700:1743	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	0	30	theme	solvent	80:86	arg1	processes					104:112	solvent free extraction processes	80:112	solvent free extraction processes	80:112	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.
25435398	7	31	theme	ultrasound	1233:1242	arg1	power					1244:1248	ultrasound power	1233:1248	ultrasound power	1233:1248	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	11	32	theme	recycled	1704:1711	arg1	water					1723:1727	the recycled "in situ" water	1700:1727	the recycled "in situ" water of citrus peels	1700:1743	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	11	33	theme	whole	1909:1913	arg1	process					1915:1921	the whole process	1905:1921	the whole process intensified in term of time and energy saving, cleanliness and reduced waste water	1905:2004	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	0	34	theme	orange	16:21	arg1	waste					29:33	orange peels waste	16:33	orange peels waste	16:33	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.
25435398	11	35	theme	energy	1955:1960	arg1	saving					1962:1967	energy saving	1955:1967	energy saving	1955:1967	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	11	36	theme	"	1721:1721	arg1	water					1723:1727	the recycled "in situ" water	1700:1727	the recycled "in situ" water of citrus peels	1700:1743	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	0	37	theme	waste	29:33	arg1	Bio-refinery					0:11	Bio-refinery	0:11	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.	0:203	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.
25435398	11	38	theme	time	1946:1949	arg1	term					1938:1941	term	1938:1941	term of time and energy saving, cleanliness and reduced waste water	1938:2004	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	4	39	theme	oil	729:731	arg1	extraction					705:714	extraction	705:714	extraction of essential oil	705:731	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	7	40	theme	statistical	1170:1180	arg1	analysis					1182:1189	The statistical analysis	1166:1189	The statistical analysis	1166:1189	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	1	41	used	used	425:428	arg2	solvent					433:439	solvent	433:439	solvent	433:439	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	1	41	used	used	425:428	arg2	water					396:400	"in situ" water	386:400	"in situ" water which was recycled and used as solvent	386:439	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	5	42	theme	ultrasound-assisted	905:923	arg1	UAE					937:939	UAE	937:939	UAE	937:939	Polyphenols extraction was performed by ultrasound-assisted extraction (UAE) and conventional extraction (CE).
25435398	5	42	theme	ultrasound-assisted	905:923	arg1	extraction					925:934	ultrasound-assisted extraction	905:934	ultrasound-assisted extraction (UAE)	905:940	Polyphenols extraction was performed by ultrasound-assisted extraction (UAE) and conventional extraction (CE).
25435398	11	43	from	compounds	1782:1790	arg1	time					1803:1806	shorter time	1795:1806	shorter time	1795:1806	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	0	44	theme	essential	166:174	arg1	oil					176:178	essential oil	166:178	essential oil	166:178	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.
25435398	4	45	used	used	792:795	arg2	solvent					800:806	solvent	800:806	solvent	800:806	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	4	45	used	used	792:795	arg2	water					743:747	residual water	734:747	residual water of plant obtained after MHG extraction	734:786	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	9	46	theme	assisted	1492:1499	arg1	extraction					1501:1510	conventional and microwave assisted extraction	1465:1510	conventional and microwave assisted extraction	1465:1510	Pectin was extracted by conventional and microwave assisted extraction.
25435398	6	47	theme	surface	985:991	arg1	RSM					1006:1008	RSM	1006:1008	RSM	1006:1008	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	6	47	theme	surface	985:991	arg1	methodology					993:1003	Response surface methodology	976:1003	Response surface methodology (RSM) using central composite designs (CCD) approach	976:1056	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	11	48	theme	shorter	1795:1801	arg1	time					1803:1806	shorter time	1795:1806	shorter time	1795:1806	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	1	49	theme	essential	234:242	arg1	oil					244:246	essential oil	234:246	essential oil	234:246	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	7	50	theme	g	1341:1341	arg1	dm					1343:1344	50.02 mgGA/100 g dm	1326:1344	50.02 mgGA/100 g dm	1326:1344	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	7	51	theme	50.02	1326:1330	arg1	dm					1343:1344	50.02 mgGA/100 g dm	1326:1344	50.02 mgGA/100 g dm	1326:1344	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	7	52	theme	polyphenol	1306:1315	arg1	yield					1317:1321	a polyphenol yield	1304:1321	a polyphenol yield of 50.02 mgGA/100 g dm	1304:1344	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	2	53	theme	steam	557:561	arg1	SD					588:589	SD	588:589	SD	588:589	The essential oil extraction performed by Microwave Hydrodiffusion and Gravity (MHG) was optimized and compared to steam distillation extraction (SD).
25435398	2	53	theme	steam	557:561	arg1	extraction					576:585	steam distillation extraction	557:585	steam distillation extraction (SD)	557:590	The essential oil extraction performed by Microwave Hydrodiffusion and Gravity (MHG) was optimized and compared to steam distillation extraction (SD).
25435398	6	54	theme	designs	1035:1041	arg1	approach					1049:1056	central composite designs (CCD) approach	1017:1056	central composite designs (CCD) approach	1017:1056	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	3	55	theme	±	661:661	arg1	%					667:667	4.16 ± 0.05%	656:667	4.16 ± 0.05% for MHG and SD	656:682	No significant changes in yield were noticed: 4.22 ± 0.03% and 4.16 ± 0.05% for MHG and SD, respectively.
25435398	2	56	theme	Microwave	484:492	arg1	Hydrodiffusion					494:507	Microwave Hydrodiffusion	484:507	Microwave Hydrodiffusion	484:507	The essential oil extraction performed by Microwave Hydrodiffusion and Gravity (MHG) was optimized and compared to steam distillation extraction (SD).
25435398	0	57	theme	microwave	135:143	arg1	techniques					145:154	microwave techniques	135:154	microwave techniques	135:154	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.
25435398	11	58	theme	peels	1739:1743	arg1	ultrasound					1685:1694	ultrasound	1685:1694	ultrasound	1685:1694	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	11	58	theme	peels	1739:1743	arg1	microwave					1674:1682	microwave	1674:1682	microwave	1674:1682	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	11	58	theme	peels	1739:1743	arg1	water					1723:1727	the recycled "in situ" water	1700:1727	the recycled "in situ" water of citrus peels	1700:1743	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	6	59	theme	process	1103:1109	arg1	variables					1111:1119	process variables	1103:1119	process variables	1103:1119	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	5	60	theme	conventional	946:957	arg1	extraction					959:968	conventional extraction	946:968	conventional extraction (CE)	946:973	Polyphenols extraction was performed by ultrasound-assisted extraction (UAE) and conventional extraction (CE).
25435398	5	60	theme	conventional	946:957	arg1	CE					971:972	CE	971:972	CE	971:972	Polyphenols extraction was performed by ultrasound-assisted extraction (UAE) and conventional extraction (CE).
25435398	11	61	theme	added	1769:1773	arg1	compounds					1782:1790	high added values compounds	1764:1790	high added values compounds in shorter time	1764:1806	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	11	62	theme	natural	1853:1859	arg1	resources					1861:1869	only natural resources	1848:1869	only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water	1848:2004	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	11	63	theme	reduced	1986:1992	arg1	water					2000:2004	reduced waste water	1986:2004	reduced waste water	1986:2004	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	11	63	theme	reduced	1986:1992	arg1	time					1946:1949	time	1946:1949	time	1946:1949	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	0	64	theme	integrated	59:68	arg1	green					70:74	integrated green	59:74	integrated green	59:74	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.
25435398	1	65	theme	microwave	314:322	arg1	technology					339:348	microwave and ultrasound technology	314:348	technology	339:348	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	4	66	theme	pectin	828:833	arg1	extraction					835:844	pectin extraction	828:844	pectin extraction from MHG residues	828:862	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	0	67	theme	free	88:91	arg1	processes					104:112	solvent free extraction processes	80:112	solvent free extraction processes	80:112	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.
25435398	1	68	theme	ultrasound	328:337	arg1	technology					339:348	microwave and ultrasound technology	314:348	technology	339:348	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	5	69	theme	Polyphenols	865:875	arg1	extraction					877:886	Polyphenols extraction	865:886	Polyphenols extraction	865:886	Polyphenols extraction was performed by ultrasound-assisted extraction (UAE) and conventional extraction (CE).
25435398	6	70	theme	Response	976:983	arg1	RSM					1006:1008	RSM	1006:1008	RSM	1006:1008	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	6	70	theme	Response	976:983	arg1	methodology					993:1003	Response surface methodology	976:1003	Response surface methodology (RSM) using central composite designs (CCD) approach	976:1056	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	2	71	theme	oil	456:458	arg1	extraction					460:469	The essential oil extraction	442:469	The essential oil extraction performed by Microwave Hydrodiffusion and Gravity (MHG)	442:525	The essential oil extraction performed by Microwave Hydrodiffusion and Gravity (MHG) was optimized and compared to steam distillation extraction (SD).
25435398	11	72	theme	waste	1994:1998	arg1	water					2000:2004	reduced waste water	1986:2004	reduced waste water	1986:2004	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	11	72	theme	waste	1994:1998	arg1	time					1946:1949	time	1946:1949	time	1946:1949	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	7	73	theme	optimized	1209:1217	arg1	conditions					1219:1228	the optimized conditions	1205:1228	the optimized conditions of ultrasound power and temperature	1205:1264	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	7	73	theme	optimized	1209:1217	arg1	W/cm					1277:1280	0.956 W/cm	1271:1280	0.956 W/cm(2)	1271:1283	The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm.
25435398	0	74	theme	peels	23:27	arg1	waste					29:33	orange peels waste	16:33	orange peels waste	16:33	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.
25435398	1	75	theme	polyphenols	249:259	arg1	extraction					220:229	extraction	220:229	extraction of essential oil, polyphenols and pectin from orange peel	220:287	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	11	76	theme	citrus	1732:1737	arg1	peels					1739:1743	citrus peels	1732:1743	citrus peels	1732:1743	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	1	77	theme	pectin	265:270	arg1	extraction					220:229	extraction	220:229	extraction of essential oil, polyphenols and pectin from orange peel	220:287	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	11	78	theme	saving	1962:1967	arg1	term					1938:1941	term	1938:1941	term of time and energy saving, cleanliness and reduced waste water	1938:2004	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	8	79	theme	%	1425:1425	arg1	increase					1411:1418	an increase	1408:1418	an increase of 30% in TPC yield	1408:1438	Compared with the conventional extraction (CE), the UAE gave an increase of 30% in TPC yield.
25435398	11	80	theme	water	1723:1727	arg1	Combination					1659:1669	Combination	1659:1669	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels	1659:1743	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	0	81	theme	new	38:40	arg1	concept					42:48	a new concept	36:48	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.	0:203	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.
25435398	1	82	theme	orange	277:282	arg1	peel					284:287	orange peel	277:287	orange peel	277:287	In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent.
25435398	11	83	dep	in	1714:1715	arg1	situ					1717:1720	situ	1717:1720	situ	1717:1720	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	6	84	theme	composite	1025:1033	arg1	CCD					1044:1046	CCD	1044:1046	CCD	1044:1046	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	6	84	theme	composite	1025:1033	arg1	designs					1035:1041	central composite designs	1017:1041	central composite designs (CCD) approach	1017:1056	Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE).
25435398	11	85	theme	closed	1830:1835	arg1	loop					1837:1840	a closed loop	1828:1840	a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water	1828:2004	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	8	86	theme	TPC	1430:1432	arg1	yield					1434:1438	TPC yield	1430:1438	TPC yield	1430:1438	Compared with the conventional extraction (CE), the UAE gave an increase of 30% in TPC yield.
25435398	4	87	theme	essential	719:727	arg1	oil					729:731	essential oil	719:731	essential oil	719:731	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	11	88	theme	microwave	1674:1682	arg1	Combination					1659:1669	Combination	1659:1669	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels	1659:1743	Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water.
25435398	0	89	dep	Bio-refinery	0:11	arg1	concept					42:48	a new concept	36:48	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.	0:203	Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.
25435398	3	90	theme	significant	596:606	arg1	changes					608:614	No significant changes	593:614	No significant changes in yield	593:623	No significant changes in yield were noticed: 4.22 ± 0.03% and 4.16 ± 0.05% for MHG and SD, respectively.
25435398	10	91	theme	maximal	1536:1542	arg1	yield					1544:1548	a maximal yield	1534:1548	a maximal yield of 24.2% for microwave power of 500 W	1534:1586	This technique gives a maximal yield of 24.2% for microwave power of 500 W in only 3 min whereas conventional extraction gives 18.32% in 120 min.
25435398	9	92	theme	conventional	1465:1476	arg1	extraction					1501:1510	conventional and microwave assisted extraction	1465:1510	conventional and microwave assisted extraction	1465:1510	Pectin was extracted by conventional and microwave assisted extraction.
25435398	3	93	from	changes	608:614	arg1	yield					619:623	yield	619:623	yield	619:623	No significant changes in yield were noticed: 4.22 ± 0.03% and 4.16 ± 0.05% for MHG and SD, respectively.
25435398	10	94	theme	microwave	1563:1571	arg1	power					1573:1577	microwave power	1563:1577	microwave power of 500 W	1563:1586	This technique gives a maximal yield of 24.2% for microwave power of 500 W in only 3 min whereas conventional extraction gives 18.32% in 120 min.
25435398	4	95	from	residues	855:862	arg1	polyphenols					812:822	polyphenols	812:822	polyphenols	812:822	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
25435398	4	95	from	residues	855:862	arg1	extraction					835:844	pectin extraction	828:844	pectin extraction from MHG residues	828:862	After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues.
26772799	12	0	theme	cartilage	1852:1860	arg1	efforts					1865:1871	cartilage TE efforts	1852:1871	cartilage TE efforts	1852:1871	In future, this data can be used in AUR cartilage TE efforts to support reproduction of tissue-specific mechanical properties.
26772799	10	1	theme	mechanical	1621:1630	arg1	integrity					1632:1640	all compressive mechanical integrity	1605:1640	all compressive mechanical integrity	1605:1640	Auricular cartilage without elastin lost all compressive mechanical integrity, whereas in articular cartilage this was provided by collagen.
26772799	2	2	theme	AUR	354:356	arg1	cartilage					359:367	elastin-rich auricular (AUR) cartilage	330:367	elastin-rich auricular (AUR) cartilage	330:367	On the other hand, very little information about other cartilage tissues is available, such as elastin-rich auricular (AUR) cartilage.
26772799	8	3	theme	viscous	1232:1238	arg1	dissipation					1240:1250	limited viscous dissipation	1224:1250	limited viscous dissipation	1224:1250	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	7	4	theme	elastic	1172:1178	arg1	behavior					1180:1187	a more elastic behavior	1165:1187	a more elastic behavior	1165:1187	ART and AUR cartilages showed different viscoelastic behaviors, with AUR cartilage exhibiting a more elastic behavior.
26772799	3	5	theme	in-depth	547:554	arg1	analysis					556:563	in-depth analysis	547:563	in-depth analysis of AUR cartilage properties	547:591	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	8	6	theme	ART	1307:1309	arg1	cartilage					1311:1319	ART cartilage	1307:1319	ART cartilage	1307:1319	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	6	7	theme	Elastase	822:829	arg1	incubation					831:840	Elastase incubation	822:840	Elastase incubation	822:840	Elastase incubation was performed to simultaneously deplete elastin and sulfated glycosaminoglycans (sGAG), while hyaluronidase incubation was used to deplete sGAG-only, in order to systematically investigate matrix components in material behavior.
26772799	9	8	theme	GAG	1508:1510	arg1	loss					1512:1515	GAG loss	1508:1515	GAG loss	1508:1515	In conclusion, loss of sGAG had no effect on auricular mechanics in contrast to articular cartilage where GAG loss clearly correlated with mechanical properties.
26772799	8	9	theme	energy	1262:1267	arg1	properties					1209:1218	Higher equilibrium properties	1190:1218	Higher equilibrium properties	1190:1218	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	8	9	theme	energy	1262:1267	arg1	dissipation					1240:1250	limited viscous dissipation	1224:1250	limited viscous dissipation	1224:1250	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	3	10	theme	cartilage	572:580	arg1	properties					582:591	AUR cartilage properties	568:591	AUR cartilage properties	568:591	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	8	11	theme	rapid	1333:1337	arg1	response					1347:1354	a rapid viscous response	1331:1354	a rapid viscous response	1331:1354	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	5	12	theme	Stress-relaxation	726:742	arg1	indentation					744:754	Stress-relaxation indentation and tensile tests	726:772	indentation	744:754	Stress-relaxation indentation and tensile tests were performed on bovine ART and AUR cartilage.
26772799	1	13	theme	mechanical	212:221	arg1	properties					223:232	mechanical properties	212:232	mechanical properties	212:232	It is well-accepted that articular (ART) cartilage composition and tissue architecture are intimately related to mechanical properties.
26772799	8	14	theme	AUR	1286:1288	arg1	cartilage					1290:1298	AUR cartilage	1286:1298	AUR cartilage	1286:1298	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	4	15	theme	AUR	663:665	arg1	cartilage					667:675	AUR cartilage	663:675	AUR cartilage	663:675	This study aims to explore the constituent-function relationships of AUR cartilage, and how elastin influences mechanical behavior.
26772799	2	16	theme	other	242:246	arg1	hand					248:251	the other hand	238:251	the other hand	238:251	On the other hand, very little information about other cartilage tissues is available, such as elastin-rich auricular (AUR) cartilage.
26772799	7	17	theme	ART	1071:1073	arg1	cartilages					1083:1092	ART and AUR cartilages	1071:1092	ART and AUR cartilages	1071:1092	ART and AUR cartilages showed different viscoelastic behaviors, with AUR cartilage exhibiting a more elastic behavior.
26772799	8	18	theme	high	1360:1363	arg1	resistance					1365:1374	high resistance	1360:1374	high resistance to instantaneous loading	1360:1399	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	7	19	theme	different	1101:1109	arg1	behaviors					1124:1132	different viscoelastic behaviors	1101:1132	different viscoelastic behaviors	1101:1132	ART and AUR cartilages showed different viscoelastic behaviors, with AUR cartilage exhibiting a more elastic behavior.
26772799	9	20	dep	cartilage	1492:1500	arg1	contrast					1470:1477	contrast	1470:1477	contrast	1470:1477	In conclusion, loss of sGAG had no effect on auricular mechanics in contrast to articular cartilage where GAG loss clearly correlated with mechanical properties.
26772799	2	21	theme	little	259:264	arg1	information					266:276	very little information	254:276	very little information about other cartilage tissues	254:306	On the other hand, very little information about other cartilage tissues is available, such as elastin-rich auricular (AUR) cartilage.
26772799	5	22	theme	bovine	792:797	arg1	ART					799:801	bovine ART	792:801	bovine ART	792:801	Stress-relaxation indentation and tensile tests were performed on bovine ART and AUR cartilage.
26772799	9	23	from	effect	1437:1442	arg1	mechanics					1457:1465	auricular mechanics	1447:1465	auricular mechanics	1447:1465	In conclusion, loss of sGAG had no effect on auricular mechanics in contrast to articular cartilage where GAG loss clearly correlated with mechanical properties.
26772799	1	24	theme	tissue	166:171	arg1	architecture					173:184	tissue architecture	166:184	tissue architecture	166:184	It is well-accepted that articular (ART) cartilage composition and tissue architecture are intimately related to mechanical properties.
26772799	2	25	theme	cartilage	290:298	arg1	tissues					300:306	other cartilage tissues	284:306	other cartilage tissues	284:306	On the other hand, very little information about other cartilage tissues is available, such as elastin-rich auricular (AUR) cartilage.
26772799	6	26	theme	matrix	1031:1036	arg1	components					1038:1047	matrix components	1031:1047	matrix components in material behavior	1031:1068	Elastase incubation was performed to simultaneously deplete elastin and sulfated glycosaminoglycans (sGAG), while hyaluronidase incubation was used to deplete sGAG-only, in order to systematically investigate matrix components in material behavior.
26772799	6	27	from	components	1038:1047	arg1	behavior					1061:1068	material behavior	1052:1068	material behavior	1052:1068	Elastase incubation was performed to simultaneously deplete elastin and sulfated glycosaminoglycans (sGAG), while hyaluronidase incubation was used to deplete sGAG-only, in order to systematically investigate matrix components in material behavior.
26772799	3	28	theme	osteoarthritis	429:442	arg1	research					444:451	osteoarthritis research	429:451	osteoarthritis research	429:451	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	0	29	theme	Tissue	0:5	arg1	composition					7:17	Tissue composition	0:17	Tissue composition	0:17	Tissue composition regulates distinct viscoelastic responses in auricular and articular cartilage.
26772799	3	30	theme	similar	530:536	arg1	way					538:540	a similar way	528:540	a similar way	528:540	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	10	31	theme	Auricular	1564:1572	arg1	cartilage					1574:1582	Auricular cartilage	1564:1582	Auricular cartilage without elastin	1564:1598	Auricular cartilage without elastin lost all compressive mechanical integrity, whereas in articular cartilage this was provided by collagen.
26772799	3	32	theme	cartilage	458:466	arg1	reconstruction					468:481	ear cartilage reconstruction	454:481	ear cartilage reconstruction	454:481	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	0	33	theme	viscoelastic	38:49	arg1	responses					51:59	distinct viscoelastic responses	29:59	distinct viscoelastic responses	29:59	Tissue composition regulates distinct viscoelastic responses in auricular and articular cartilage.
26772799	9	34	theme	sGAG	1425:1428	arg1	loss					1417:1420	loss	1417:1420	loss of sGAG	1417:1428	In conclusion, loss of sGAG had no effect on auricular mechanics in contrast to articular cartilage where GAG loss clearly correlated with mechanical properties.
26772799	3	35	theme	ART	402:404	arg1	cartilage					406:414	ART cartilage	402:414	ART cartilage	402:414	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	10	36	theme	compressive	1609:1619	arg1	integrity					1632:1640	all compressive mechanical integrity	1605:1640	all compressive mechanical integrity	1605:1640	Auricular cartilage without elastin lost all compressive mechanical integrity, whereas in articular cartilage this was provided by collagen.
26772799	2	37	theme	elastin-rich	330:341	arg1	cartilage					359:367	elastin-rich auricular (AUR) cartilage	330:367	elastin-rich auricular (AUR) cartilage	330:367	On the other hand, very little information about other cartilage tissues is available, such as elastin-rich auricular (AUR) cartilage.
26772799	12	38	theme	mechanical	1916:1925	arg1	properties					1927:1936	tissue-specific mechanical properties	1900:1936	tissue-specific mechanical properties	1900:1936	In future, this data can be used in AUR cartilage TE efforts to support reproduction of tissue-specific mechanical properties.
26772799	11	39	theme	ear	1797:1799	arg1	cartilage					1801:1809	ear cartilage	1797:1809	ear cartilage	1797:1809	This work shows for the first time the involvement of elastin in the mechanical behavior of ear cartilage.
26772799	8	40	theme	Higher	1190:1195	arg1	properties					1209:1218	Higher equilibrium properties	1190:1218	Higher equilibrium properties	1190:1218	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	12	41	theme	TE	1862:1863	arg1	efforts					1865:1871	cartilage TE efforts	1852:1871	cartilage TE efforts	1852:1871	In future, this data can be used in AUR cartilage TE efforts to support reproduction of tissue-specific mechanical properties.
26772799	9	42	theme	articular	1482:1490	arg1	cartilage					1492:1500	articular cartilage	1482:1500	articular cartilage where GAG loss clearly correlated with mechanical properties	1482:1561	In conclusion, loss of sGAG had no effect on auricular mechanics in contrast to articular cartilage where GAG loss clearly correlated with mechanical properties.
26772799	8	43	theme	limited	1224:1230	arg1	dissipation					1240:1250	limited viscous dissipation	1224:1250	limited viscous dissipation	1224:1250	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	6	44	theme	hyaluronidase	936:948	arg1	incubation					950:959	hyaluronidase incubation	936:959	hyaluronidase incubation	936:959	Elastase incubation was performed to simultaneously deplete elastin and sulfated glycosaminoglycans (sGAG), while hyaluronidase incubation was used to deplete sGAG-only, in order to systematically investigate matrix components in material behavior.
26772799	10	45	theme	articular	1654:1662	arg1	cartilage					1664:1672	articular cartilage	1654:1672	articular cartilage	1654:1672	Auricular cartilage without elastin lost all compressive mechanical integrity, whereas in articular cartilage this was provided by collagen.
26772799	11	46	theme	elastin	1759:1765	arg1	involvement					1744:1754	the involvement	1740:1754	the involvement of elastin in the mechanical behavior of ear cartilage	1740:1809	This work shows for the first time the involvement of elastin in the mechanical behavior of ear cartilage.
26772799	2	47	theme	other	284:288	arg1	tissues					300:306	other cartilage tissues	284:306	other cartilage tissues	284:306	On the other hand, very little information about other cartilage tissues is available, such as elastin-rich auricular (AUR) cartilage.
26772799	12	48	used	used	1840:1843	arg2	data					1828:1831	this data	1823:1831	this data	1823:1831	In future, this data can be used in AUR cartilage TE efforts to support reproduction of tissue-specific mechanical properties.
26772799	4	49	theme	cartilage	667:675	arg1	relationships					646:658	the constituent-function relationships	621:658	the constituent-function relationships of AUR cartilage	621:675	This study aims to explore the constituent-function relationships of AUR cartilage, and how elastin influences mechanical behavior.
26772799	7	50	theme	AUR	1140:1142	arg1	cartilage					1144:1152	AUR cartilage	1140:1152	AUR cartilage exhibiting a more elastic behavior	1140:1187	ART and AUR cartilages showed different viscoelastic behaviors, with AUR cartilage exhibiting a more elastic behavior.
26772799	3	51	theme	AUR	568:570	arg1	properties					582:591	AUR cartilage properties	568:591	AUR cartilage properties	568:591	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	8	52	theme	strain	1255:1260	arg1	energy					1262:1267	strain energy	1255:1267	strain energy	1255:1267	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	4	53	theme	constituent-function	625:644	arg1	relationships					646:658	the constituent-function relationships	621:658	the constituent-function relationships of AUR cartilage	621:675	This study aims to explore the constituent-function relationships of AUR cartilage, and how elastin influences mechanical behavior.
26772799	7	54	theme	viscoelastic	1111:1122	arg1	behaviors					1124:1132	different viscoelastic behaviors	1101:1132	different viscoelastic behaviors	1101:1132	ART and AUR cartilages showed different viscoelastic behaviors, with AUR cartilage exhibiting a more elastic behavior.
26772799	9	55	theme	mechanical	1541:1550	arg1	properties					1552:1561	mechanical properties	1541:1561	mechanical properties	1541:1561	In conclusion, loss of sGAG had no effect on auricular mechanics in contrast to articular cartilage where GAG loss clearly correlated with mechanical properties.
26772799	8	56	theme	viscous	1339:1345	arg1	response					1347:1354	a rapid viscous response	1331:1354	a rapid viscous response	1331:1354	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	3	57	theme	properties	582:591	arg1	analysis					556:563	in-depth analysis	547:563	in-depth analysis of AUR cartilage properties	547:591	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	1	58	theme	articular	124:132	arg1	composition					150:160	articular (ART) cartilage composition	124:160	articular (ART) cartilage composition	124:160	It is well-accepted that articular (ART) cartilage composition and tissue architecture are intimately related to mechanical properties.
26772799	7	59	theme	AUR	1079:1081	arg1	cartilages					1083:1092	ART and AUR cartilages	1071:1092	ART and AUR cartilages	1071:1092	ART and AUR cartilages showed different viscoelastic behaviors, with AUR cartilage exhibiting a more elastic behavior.
26772799	1	60	theme	ART	135:137	arg1	composition					150:160	articular (ART) cartilage composition	124:160	articular (ART) cartilage composition	124:160	It is well-accepted that articular (ART) cartilage composition and tissue architecture are intimately related to mechanical properties.
26772799	0	61	theme	articular	78:86	arg1	cartilage					88:96	articular cartilage	78:96	articular cartilage	78:96	Tissue composition regulates distinct viscoelastic responses in auricular and articular cartilage.
26772799	4	62	dep	aims	605:608	arg1	influences					694:703	influences	694:703	influences mechanical behavior	694:723	This study aims to explore the constituent-function relationships of AUR cartilage, and how elastin influences mechanical behavior.
26772799	1	63	theme	cartilage	140:148	arg1	composition					150:160	articular (ART) cartilage composition	124:160	articular (ART) cartilage composition	124:160	It is well-accepted that articular (ART) cartilage composition and tissue architecture are intimately related to mechanical properties.
26772799	8	64	theme	instantaneous	1379:1391	arg1	loading					1393:1399	instantaneous loading	1379:1399	instantaneous loading	1379:1399	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	6	65	theme	material	1052:1059	arg1	behavior					1061:1068	material behavior	1052:1068	material behavior	1052:1068	Elastase incubation was performed to simultaneously deplete elastin and sulfated glycosaminoglycans (sGAG), while hyaluronidase incubation was used to deplete sGAG-only, in order to systematically investigate matrix components in material behavior.
26772799	4	66	theme	mechanical	705:714	arg1	behavior					716:723	mechanical behavior	705:723	mechanical behavior	705:723	This study aims to explore the constituent-function relationships of AUR cartilage, and how elastin influences mechanical behavior.
26772799	5	67	theme	tensile	760:766	arg1	tests					768:772	Stress-relaxation indentation and tensile tests	726:772	tests	768:772	Stress-relaxation indentation and tensile tests were performed on bovine ART and AUR cartilage.
26772799	6	68	used	used	965:968	arg2	incubation					950:959	hyaluronidase incubation	936:959	hyaluronidase incubation	936:959	Elastase incubation was performed to simultaneously deplete elastin and sulfated glycosaminoglycans (sGAG), while hyaluronidase incubation was used to deplete sGAG-only, in order to systematically investigate matrix components in material behavior.
26772799	3	69	theme	ear	454:456	arg1	reconstruction					468:481	ear cartilage reconstruction	454:481	ear cartilage reconstruction	454:481	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	11	70	from	involvement	1744:1754	arg1	behavior					1785:1792	the mechanical behavior	1770:1792	the mechanical behavior of ear cartilage	1770:1809	This work shows for the first time the involvement of elastin in the mechanical behavior of ear cartilage.
26772799	0	71	theme	distinct	29:36	arg1	responses					51:59	distinct viscoelastic responses	29:59	distinct viscoelastic responses	29:59	Tissue composition regulates distinct viscoelastic responses in auricular and articular cartilage.
26772799	3	72	theme	thorough	376:383	arg1	investigation					385:397	thorough investigation	376:397	thorough investigation of ART cartilage	376:414	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	11	73	theme	mechanical	1774:1783	arg1	behavior					1785:1792	the mechanical behavior	1770:1792	the mechanical behavior of ear cartilage	1770:1809	This work shows for the first time the involvement of elastin in the mechanical behavior of ear cartilage.
26772799	3	74	theme	tissue	487:492	arg1	TE					507:508	TE	507:508	TE	507:508	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	3	74	theme	tissue	487:492	arg1	engineering					494:504	tissue engineering	487:504	tissue engineering (TE)	487:509	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	5	75	theme	AUR	807:809	arg1	cartilage					811:819	AUR cartilage	807:819	AUR cartilage	807:819	Stress-relaxation indentation and tensile tests were performed on bovine ART and AUR cartilage.
26772799	12	76	theme	tissue-specific	1900:1914	arg1	properties					1927:1936	tissue-specific mechanical properties	1900:1936	tissue-specific mechanical properties	1900:1936	In future, this data can be used in AUR cartilage TE efforts to support reproduction of tissue-specific mechanical properties.
26772799	6	77	theme	sulfated	894:901	arg1	glycosaminoglycans					903:920	sulfated glycosaminoglycans	894:920	sulfated glycosaminoglycans (sGAG)	894:927	Elastase incubation was performed to simultaneously deplete elastin and sulfated glycosaminoglycans (sGAG), while hyaluronidase incubation was used to deplete sGAG-only, in order to systematically investigate matrix components in material behavior.
26772799	6	77	theme	sulfated	894:901	arg1	sGAG					923:926	sGAG	923:926	sGAG	923:926	Elastase incubation was performed to simultaneously deplete elastin and sulfated glycosaminoglycans (sGAG), while hyaluronidase incubation was used to deplete sGAG-only, in order to systematically investigate matrix components in material behavior.
26772799	3	78	theme	cartilage	406:414	arg1	investigation					385:397	thorough investigation	376:397	thorough investigation of ART cartilage	376:414	While thorough investigation of ART cartilage has enhanced osteoarthritis research, ear cartilage reconstruction and tissue engineering (TE) could benefit in a similar way from in-depth analysis of AUR cartilage properties.
26772799	11	79	theme	cartilage	1801:1809	arg1	behavior					1785:1792	the mechanical behavior	1770:1792	the mechanical behavior of ear cartilage	1770:1809	This work shows for the first time the involvement of elastin in the mechanical behavior of ear cartilage.
26772799	8	80	located	observed	1274:1281	arg2	dissipation					1240:1250	limited viscous dissipation	1224:1250	limited viscous dissipation	1224:1250	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	8	80	located	observed	1274:1281	arg1	cartilage					1290:1298	AUR cartilage	1286:1298	AUR cartilage	1286:1298	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	8	80	located	observed	1274:1281	arg2	properties					1209:1218	Higher equilibrium properties	1190:1218	Higher equilibrium properties	1190:1218	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	2	81	theme	auricular	343:351	arg1	cartilage					359:367	elastin-rich auricular (AUR) cartilage	330:367	elastin-rich auricular (AUR) cartilage	330:367	On the other hand, very little information about other cartilage tissues is available, such as elastin-rich auricular (AUR) cartilage.
26772799	12	82	theme	properties	1927:1936	arg1	reproduction					1884:1895	reproduction	1884:1895	reproduction of tissue-specific mechanical properties	1884:1936	In future, this data can be used in AUR cartilage TE efforts to support reproduction of tissue-specific mechanical properties.
26772799	9	83	theme	auricular	1447:1455	arg1	mechanics					1457:1465	auricular mechanics	1447:1465	auricular mechanics	1447:1465	In conclusion, loss of sGAG had no effect on auricular mechanics in contrast to articular cartilage where GAG loss clearly correlated with mechanical properties.
26772799	8	84	theme	equilibrium	1197:1207	arg1	properties					1209:1218	Higher equilibrium properties	1190:1218	Higher equilibrium properties	1190:1218	Higher equilibrium properties and limited viscous dissipation of strain energy were observed in AUR cartilage, while ART cartilage exhibited a rapid viscous response and high resistance to instantaneous loading.
26772799	11	85	theme	first	1729:1733	arg1	time					1735:1738	the first time	1725:1738	the first time	1725:1738	This work shows for the first time the involvement of elastin in the mechanical behavior of ear cartilage.
26772799	9	86	contain	had	1430:1432	arg1	loss					1417:1420	loss	1417:1420	loss of sGAG	1417:1428	In conclusion, loss of sGAG had no effect on auricular mechanics in contrast to articular cartilage where GAG loss clearly correlated with mechanical properties.
26772799	9	86	contain	had	1430:1432	arg2	effect					1437:1442	no effect	1434:1442	no effect on auricular mechanics	1434:1465	In conclusion, loss of sGAG had no effect on auricular mechanics in contrast to articular cartilage where GAG loss clearly correlated with mechanical properties.
26059413	4	0	theme	supersaturation	519:533	arg1	factor					562:567	a critical factor	551:567	a critical factor	551:567	For poorly water-soluble compounds, the supersaturation behavior may be a critical factor in determining the extent of oral absorption.
26059413	4	0	theme	supersaturation	519:533	arg1	behavior					535:542	the supersaturation behavior	515:542	the supersaturation behavior	515:542	For poorly water-soluble compounds, the supersaturation behavior may be a critical factor in determining the extent of oral absorption.
26059413	10	1	theme	other	1428:1432	arg1	formulations					1450:1461	other supersaturating formulations	1428:1461	other supersaturating formulations	1428:1461	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	5	2	theme	water-soluble	719:731	arg1	compounds					733:741	several poorly water-soluble compounds	704:741	several poorly water-soluble compounds	704:741	The goal of the current study was to evaluate the maximum achievable supersaturation for several poorly water-soluble compounds alone, and in combination.
26059413	10	3	theme	physicochemical	1525:1539	arg1	interactions					1541:1552	drug-drug physicochemical interactions	1515:1552	drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract	1515:1612	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	4	4	theme	absorption	603:612	arg1	extent					588:593	the extent	584:593	the extent of oral absorption	584:612	For poorly water-soluble compounds, the supersaturation behavior may be a critical factor in determining the extent of oral absorption.
26059413	8	5	theme	amorphous	1077:1085	arg1	"					1097:1097	"amorphous solubility"	1076:1097	"amorphous solubility" of each component in aqueous solution	1076:1135	The results showed that "amorphous solubility" of each component in aqueous solution is substantially decreased by the second component, as long as the two drugs are miscible in the amorphous state.
26059413	10	6	from	tract	1608:1612	arg1	understanding					1498:1510	a broader understanding	1488:1510	a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract	1488:1612	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	5	7	theme	maximum	665:671	arg1	supersaturation					684:698	the maximum achievable supersaturation	661:698	the maximum achievable supersaturation for several poorly water-soluble compounds	661:741	The goal of the current study was to evaluate the maximum achievable supersaturation for several poorly water-soluble compounds alone, and in combination.
26059413	7	8	theme	pure	892:895	arg1	drugs					897:901	the pure drugs	888:901	the pure drugs	888:901	The "amorphous solubility" for the pure drugs was determined using different techniques and the change in this solubility was then measured in the presence of differing amounts of a second drug.
26059413	4	9	theme	oral	598:601	arg1	absorption					603:612	oral absorption	598:612	oral absorption	598:612	For poorly water-soluble compounds, the supersaturation behavior may be a critical factor in determining the extent of oral absorption.
26059413	0	10	theme	Drug	107:110	arg1	Drugs-Impact					82:93	Poorly Water-Soluble Drugs-Impact	61:93	Poorly Water-Soluble Drugs-Impact of a Second Drug	61:110	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	0	11	from	Thermodynamics	0:13	arg1	Behavior					134:141	the Solution Phase Behavior	115:141	the Solution Phase Behavior	115:141	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	0	11	from	Thermodynamics	0:13	arg1	Implications					147:158	Implications	147:158	Implications for Combination Products	147:183	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	5	12	theme	achievable	673:682	arg1	supersaturation					684:698	the maximum achievable supersaturation	661:698	the maximum achievable supersaturation for several poorly water-soluble compounds	661:741	The goal of the current study was to evaluate the maximum achievable supersaturation for several poorly water-soluble compounds alone, and in combination.
26059413	8	13	from	state	1244:1248	arg1	miscible					1218:1225	miscible	1218:1225	miscible	1218:1225	The results showed that "amorphous solubility" of each component in aqueous solution is substantially decreased by the second component, as long as the two drugs are miscible in the amorphous state.
26059413	8	13	from	state	1244:1248	arg1	drugs					1208:1212	the two drugs	1200:1212	the two drugs	1200:1212	The results showed that "amorphous solubility" of each component in aqueous solution is substantially decreased by the second component, as long as the two drugs are miscible in the amorphous state.
26059413	0	14	theme	Second	100:105	arg1	Drug					107:110	a Second Drug	98:110	a Second Drug	98:110	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	9	15	used	used	1289:1292	arg2	model					1274:1278	A simple thermodynamic model	1251:1278	A simple thermodynamic model	1251:1278	A simple thermodynamic model could be used to predict the changes in solubility as a function of composition.
26059413	4	16	theme	water-soluble	490:502	arg1	compounds					504:512	poorly water-soluble compounds	483:512	poorly water-soluble compounds	483:512	For poorly water-soluble compounds, the supersaturation behavior may be a critical factor in determining the extent of oral absorption.
26059413	5	17	theme	study	639:643	arg1	goal					619:622	The goal	615:622	The goal of the current study	615:643	The goal of the current study was to evaluate the maximum achievable supersaturation for several poorly water-soluble compounds alone, and in combination.
26059413	5	18	theme	current	631:637	arg1	study					639:643	the current study	627:643	the current study	627:643	The goal of the current study was to evaluate the maximum achievable supersaturation for several poorly water-soluble compounds alone, and in combination.
26059413	10	19	theme	in	1557:1558	arg1	assays					1566:1571	in vitro assays	1557:1571	in vitro assays	1557:1571	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	8	20	theme	second	1171:1176	arg1	component					1178:1186	the second component	1167:1186	the second component	1167:1186	The results showed that "amorphous solubility" of each component in aqueous solution is substantially decreased by the second component, as long as the two drugs are miscible in the amorphous state.
26059413	0	21	theme	Solutions	48:56	arg1	Thermodynamics					0:13	Thermodynamics	0:13	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.	0:184	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	7	22	theme	solubility	872:881	arg1	"					882:882	The "amorphous solubility"	857:882	The "amorphous solubility" for the pure drugs	857:901	The "amorphous solubility" for the pure drugs was determined using different techniques and the change in this solubility was then measured in the presence of differing amounts of a second drug.
26059413	3	23	theme	formulation	454:464	arg1	performance					466:476	formulation performance	454:476	formulation performance	454:476	This raises the possibility of interactions between different drugs that may impact formulation performance.
26059413	10	24	theme	interactions	1541:1552	arg1	understanding					1498:1510	a broader understanding	1488:1510	a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract	1488:1612	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	9	25	from	changes	1309:1315	arg1	solubility					1320:1329	solubility	1320:1329	solubility	1320:1329	A simple thermodynamic model could be used to predict the changes in solubility as a function of composition.
26059413	2	26	theme	different	318:326	arg1	products					333:340	different drug products	318:340	different drug products	318:340	Furthermore, it is also common for different drug products to be taken simultaneously.
26059413	8	27	theme	solubility	1087:1096	arg1	"					1097:1097	"amorphous solubility"	1076:1097	"amorphous solubility" of each component in aqueous solution	1076:1135	The results showed that "amorphous solubility" of each component in aqueous solution is substantially decreased by the second component, as long as the two drugs are miscible in the amorphous state.
26059413	8	28	theme	amorphous	1234:1242	arg1	state					1244:1248	the amorphous state	1230:1248	the amorphous state	1230:1248	The results showed that "amorphous solubility" of each component in aqueous solution is substantially decreased by the second component, as long as the two drugs are miscible in the amorphous state.
26059413	10	29	theme	gastrointestinal	1591:1606	arg1	tract					1608:1612	the gastrointestinal tract	1587:1612	the gastrointestinal tract	1587:1612	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	10	30	theme	supersaturating	1434:1448	arg1	formulations					1450:1461	other supersaturating formulations	1428:1461	other supersaturating formulations	1428:1461	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	10	31	theme	great	1384:1388	arg1	value					1390:1394	great value	1384:1394	great value	1384:1394	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	1	32	theme	increasing	195:204	arg1	interest					206:213	increasing interest	195:213	increasing interest in formulating combination products that contain two or more drugs	195:280	There is increasing interest in formulating combination products that contain two or more drugs.
26059413	0	33	theme	Supersaturated	25:38	arg1	Solutions					48:56	Highly Supersaturated Aqueous Solutions	18:56	Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug	18:110	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	0	34	theme	Phase	128:132	arg1	Behavior					134:141	the Solution Phase Behavior	115:141	the Solution Phase Behavior	115:141	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	7	35	theme	amorphous	862:870	arg1	"					882:882	The "amorphous solubility"	857:882	The "amorphous solubility" for the pure drugs	857:901	The "amorphous solubility" for the pure drugs was determined using different techniques and the change in this solubility was then measured in the presence of differing amounts of a second drug.
26059413	0	36	theme	Solution	119:126	arg1	Behavior					134:141	the Solution Phase Behavior	115:141	the Solution Phase Behavior	115:141	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	7	37	theme	drug	1046:1049	arg1	amounts					1026:1032	differing amounts	1016:1032	differing amounts of a second drug	1016:1049	The "amorphous solubility" for the pure drugs was determined using different techniques and the change in this solubility was then measured in the presence of differing amounts of a second drug.
26059413	7	37	theme	drug	1046:1049	arg1	drug					1046:1049	a second drug	1037:1049	a second drug	1037:1049	The "amorphous solubility" for the pure drugs was determined using different techniques and the change in this solubility was then measured in the presence of differing amounts of a second drug.
26059413	3	38	theme	different	422:430	arg1	drugs					432:436	different drugs	422:436	different drugs that may impact formulation performance	422:476	This raises the possibility of interactions between different drugs that may impact formulation performance.
26059413	8	39	from	miscible	1218:1225	arg1	state					1244:1248	the amorphous state	1230:1248	the amorphous state	1230:1248	The results showed that "amorphous solubility" of each component in aqueous solution is substantially decreased by the second component, as long as the two drugs are miscible in the amorphous state.
26059413	10	40	from	interactions	1541:1552	arg1	tract					1608:1612	the gastrointestinal tract	1587:1612	the gastrointestinal tract	1587:1612	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	10	40	from	interactions	1541:1552	arg1	assays					1566:1571	in vitro assays	1557:1571	in vitro assays	1557:1571	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	0	41	theme	Aqueous	40:46	arg1	Solutions					48:56	Highly Supersaturated Aqueous Solutions	18:56	Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug	18:110	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	4	42	theme	critical	553:560	arg1	factor					562:567	a critical factor	551:567	a critical factor	551:567	For poorly water-soluble compounds, the supersaturation behavior may be a critical factor in determining the extent of oral absorption.
26059413	4	42	theme	critical	553:560	arg1	behavior					535:542	the supersaturation behavior	515:542	the supersaturation behavior	515:542	For poorly water-soluble compounds, the supersaturation behavior may be a critical factor in determining the extent of oral absorption.
26059413	10	43	from	understanding	1498:1510	arg1	tract					1608:1612	the gastrointestinal tract	1587:1612	the gastrointestinal tract	1587:1612	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	10	43	from	understanding	1498:1510	arg1	assays					1566:1571	in vitro assays	1557:1571	in vitro assays	1557:1571	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	1	44	contain	contain	256:262	arg1	products					242:249	combination products	230:249	combination products that contain two or more drugs	230:280	There is increasing interest in formulating combination products that contain two or more drugs.
26059413	1	44	contain	contain	256:262	arg2	drugs					276:280	two or more drugs	264:280	two or more drugs	264:280	There is increasing interest in formulating combination products that contain two or more drugs.
26059413	1	45	theme	combination	230:240	arg1	products					242:249	combination products	230:249	combination products that contain two or more drugs	230:280	There is increasing interest in formulating combination products that contain two or more drugs.
26059413	3	46	theme	interactions	401:412	arg1	possibility					386:396	the possibility	382:396	the possibility of interactions between different drugs that may impact formulation performance	382:476	This raises the possibility of interactions between different drugs that may impact formulation performance.
26059413	8	47	theme	component	1107:1115	arg1	"					1097:1097	"amorphous solubility"	1076:1097	"amorphous solubility" of each component in aqueous solution	1076:1135	The results showed that "amorphous solubility" of each component in aqueous solution is substantially decreased by the second component, as long as the two drugs are miscible in the amorphous state.
26059413	10	48	theme	broader	1490:1496	arg1	understanding					1498:1510	a broader understanding	1488:1510	a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract	1488:1612	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	8	49	theme	aqueous	1120:1126	arg1	solution					1128:1135	aqueous solution	1120:1135	aqueous solution	1120:1135	The results showed that "amorphous solubility" of each component in aqueous solution is substantially decreased by the second component, as long as the two drugs are miscible in the amorphous state.
26059413	10	50	theme	drug-drug	1515:1523	arg1	interactions					1541:1552	drug-drug physicochemical interactions	1515:1552	drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract	1515:1612	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	5	51	theme	several	704:710	arg1	compounds					733:741	several poorly water-soluble compounds	704:741	several poorly water-soluble compounds	704:741	The goal of the current study was to evaluate the maximum achievable supersaturation for several poorly water-soluble compounds alone, and in combination.
26059413	7	52	theme	differing	1016:1024	arg1	amounts					1026:1032	differing amounts	1016:1032	differing amounts of a second drug	1016:1049	The "amorphous solubility" for the pure drugs was determined using different techniques and the change in this solubility was then measured in the presence of differing amounts of a second drug.
26059413	7	52	theme	differing	1016:1024	arg1	drug					1046:1049	a second drug	1037:1049	a second drug	1037:1049	The "amorphous solubility" for the pure drugs was determined using different techniques and the change in this solubility was then measured in the presence of differing amounts of a second drug.
26059413	7	53	from	change	953:958	arg1	solubility					968:977	this solubility	963:977	this solubility	963:977	The "amorphous solubility" for the pure drugs was determined using different techniques and the change in this solubility was then measured in the presence of differing amounts of a second drug.
26059413	9	54	theme	composition	1348:1358	arg1	function					1336:1343	a function	1334:1343	a function of composition	1334:1358	A simple thermodynamic model could be used to predict the changes in solubility as a function of composition.
26059413	9	54	theme	composition	1348:1358	arg1	changes					1309:1315	the changes	1305:1315	the changes in solubility	1305:1329	A simple thermodynamic model could be used to predict the changes in solubility as a function of composition.
26059413	7	55	theme	second	1039:1044	arg1	drug					1046:1049	a second drug	1037:1049	a second drug	1037:1049	The "amorphous solubility" for the pure drugs was determined using different techniques and the change in this solubility was then measured in the presence of differing amounts of a second drug.
26059413	10	56	from	assays	1566:1571	arg1	understanding					1498:1510	a broader understanding	1488:1510	a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract	1488:1612	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26059413	7	57	theme	amounts	1026:1032	arg1	presence					1004:1011	the presence	1000:1011	the presence of differing amounts of a second drug	1000:1049	The "amorphous solubility" for the pure drugs was determined using different techniques and the change in this solubility was then measured in the presence of differing amounts of a second drug.
26059413	0	58	theme	Combination	164:174	arg1	Products					176:183	Combination Products	164:183	Combination Products	164:183	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	6	59	theme	Model	770:774	arg1	compounds					776:784	Model compounds	770:784	Model compounds	770:784	Model compounds included ritonavir, lopinavir, paclitaxel, felodipine, and diclofenac.
26059413	2	60	theme	drug	328:331	arg1	products					333:340	different drug products	318:340	different drug products	318:340	Furthermore, it is also common for different drug products to be taken simultaneously.
26059413	8	61	from	"	1097:1097	arg1	solution					1128:1135	aqueous solution	1120:1135	aqueous solution	1120:1135	The results showed that "amorphous solubility" of each component in aqueous solution is substantially decreased by the second component, as long as the two drugs are miscible in the amorphous state.
26059413	0	62	theme	Drugs-Impact	82:93	arg1	Solutions					48:56	Highly Supersaturated Aqueous Solutions	18:56	Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug	18:110	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	9	63	theme	simple	1253:1258	arg1	model					1274:1278	A simple thermodynamic model	1251:1278	A simple thermodynamic model	1251:1278	A simple thermodynamic model could be used to predict the changes in solubility as a function of composition.
26059413	7	64	theme	different	924:932	arg1	techniques					934:943	different techniques	924:943	different techniques	924:943	The "amorphous solubility" for the pure drugs was determined using different techniques and the change in this solubility was then measured in the presence of differing amounts of a second drug.
26059413	0	65	theme	Water-Soluble	68:80	arg1	Drugs-Impact					82:93	Poorly Water-Soluble Drugs-Impact	61:93	Poorly Water-Soluble Drugs-Impact of a Second Drug	61:110	Thermodynamics of Highly Supersaturated Aqueous Solutions of Poorly Water-Soluble Drugs-Impact of a Second Drug on the Solution Phase Behavior and Implications for Combination Products.
26059413	9	66	theme	thermodynamic	1260:1272	arg1	model					1274:1278	A simple thermodynamic model	1251:1278	A simple thermodynamic model	1251:1278	A simple thermodynamic model could be used to predict the changes in solubility as a function of composition.
26059413	10	67	dep	in	1557:1558	arg1	vitro					1560:1564	vitro	1560:1564	vitro	1560:1564	This information is of great value when developing co-amorphous or other supersaturating formulations and should contribute to a broader understanding of drug-drug physicochemical interactions in in vitro assays as well as in the gastrointestinal tract.
26681529	4	0	theme	r	801:801	arg1	linear					793:798	linear	793:798	linear	793:798	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	4	0	theme	r	801:801	arg1	≥					803:803	r ≥ 0.9996	801:810	r ≥ 0.9996	801:810	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	4	0	theme	r	801:801	arg1	curves					781:786	the calibration curves	765:786	the calibration curves	765:786	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	2	1	theme	monosaccharide	465:478	arg1	compositions					480:491	ten monosaccharide compositions	461:491	ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides	461:591	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	4	2	from	linear	793:798	arg1	range					820:824	the range	816:824	the range of 10-2000 μmol L(-1)	816:846	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	0	3	theme	liquid	134:139	arg1	chromatography					141:154	high-performance liquid chromatography	117:154	high-performance liquid chromatography	117:154	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.
26681529	0	4	with	microextraction	96:110	arg1	chromatography					141:154	high-performance liquid chromatography	117:154	high-performance liquid chromatography	117:154	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.
26681529	2	5	theme	liquid	398:403	arg1	chromatography					405:418	high-performance liquid chromatography	381:418	high-performance liquid chromatography	381:418	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	5	6	theme	%	967:967	arg1	range					949:953	the range	945:953	the range	945:953	The limits of detection were in the range of 0.04-1.58 μmol L(-1) , and the recoveries were in the range of 92.1-99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.
26681529	5	6	theme	%	967:967	arg1	range					886:890	the range	882:890	the range of 0.04-1.58 μmol L(-1)	882:914	The limits of detection were in the range of 0.04-1.58 μmol L(-1) , and the recoveries were in the range of 92.1-99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.
26681529	0	7	theme	high-performance	117:132	arg1	chromatography					141:154	high-performance liquid chromatography	117:154	high-performance liquid chromatography	117:154	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.
26681529	4	8	theme	μmol	837:840	arg1	L					842:842	10-2000 μmol L	829:842	10-2000 μmol L(-1)	829:846	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	4	8	theme	μmol	837:840	arg1	-1					844:845	-1	844:845	-1	844:845	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	2	9	theme	high-performance	381:396	arg1	chromatography					405:418	high-performance liquid chromatography	381:418	high-performance liquid chromatography	381:418	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	4	10	dep	linear	793:798	arg1	linear					793:798	linear	793:798	linear	793:798	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	4	10	dep	linear	793:798	arg1	≥					803:803	r ≥ 0.9996	801:810	r ≥ 0.9996	801:810	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	4	10	dep	linear	793:798	arg1	curves					781:786	the calibration curves	765:786	the calibration curves	765:786	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	5	11	theme	μmol	905:908	arg1	L					910:910	0.04-1.58 μmol L	895:910	0.04-1.58 μmol L(-1)	895:914	The limits of detection were in the range of 0.04-1.58 μmol L(-1) , and the recoveries were in the range of 92.1-99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.
26681529	5	11	theme	μmol	905:908	arg1	-1					912:913	-1	912:913	-1	912:913	The limits of detection were in the range of 0.04-1.58 μmol L(-1) , and the recoveries were in the range of 92.1-99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.
26681529	2	12	theme	polysaccharides	577:591	arg1	compositions					480:491	ten monosaccharide compositions	461:491	ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides	461:591	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	2	13	dep	compositions	480:491	arg1	sugars					545:550	six neutral sugars	533:550	six neutral sugars	533:550	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	2	13	dep	compositions	480:491	arg1	acids					505:509	two uronic acids	494:509	two uronic acids	494:509	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	2	13	dep	compositions	480:491	arg1	sugars					522:527	two amino sugars	512:527	two amino sugars	512:527	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	1	14	theme	structure	237:245	arg1	elucidation					247:257	structure elucidation	237:257	structure elucidation	237:257	The monosaccharide compositions of functional polysaccharides are essential for structure elucidation and biological activity determination.
26681529	0	15	theme	composition	15:25	arg1	analysis					27:34	Monosaccharide composition analysis	0:34	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.	0:155	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.
26681529	3	16	theme	fiber	667:671	arg1	lumen					649:653	the lumen	645:653	the lumen of a hollow fiber immersed in butyl ether	645:695	After derivatization, the sample was injected into the lumen of a hollow fiber immersed in butyl ether and separated by liquid chromatography.
26681529	2	17	theme	uronic	498:503	arg1	acids					505:509	two uronic acids	494:509	two uronic acids	494:509	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	0	18	theme	Monosaccharide	0:13	arg1	analysis					27:34	Monosaccharide composition analysis	0:34	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.	0:155	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.
26681529	4	19	from	range	820:824	arg1	linear					793:798	linear	793:798	linear	793:798	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	4	19	from	range	820:824	arg1	≥					803:803	r ≥ 0.9996	801:810	r ≥ 0.9996	801:810	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	4	19	from	range	820:824	arg1	curves					781:786	the calibration curves	765:786	the calibration curves	765:786	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	4	20	theme	L	842:842	arg1	range					820:824	the range	816:824	the range of 10-2000 μmol L(-1)	816:846	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	4	21	theme	calibration	769:779	arg1	linear					793:798	linear	793:798	linear	793:798	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	4	21	theme	calibration	769:779	arg1	≥					803:803	r ≥ 0.9996	801:810	r ≥ 0.9996	801:810	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	4	21	theme	calibration	769:779	arg1	curves					781:786	the calibration curves	765:786	the calibration curves	765:786	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	2	22	theme	neutral	537:543	arg1	sugars					545:550	six neutral sugars	533:550	six neutral sugars	533:550	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	5	23	theme	detection	864:872	arg1	limits					854:859	The limits	850:859	The limits of detection	850:872	The limits of detection were in the range of 0.04-1.58 μmol L(-1) , and the recoveries were in the range of 92.1-99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.
26681529	1	24	theme	biological	263:272	arg1	determination					283:295	biological activity determination	263:295	biological activity determination	263:295	The monosaccharide compositions of functional polysaccharides are essential for structure elucidation and biological activity determination.
26681529	5	25	theme	various	1065:1071	arg1	polysaccharides					1073:1087	various polysaccharides	1065:1087	various polysaccharides	1065:1087	The limits of detection were in the range of 0.04-1.58 μmol L(-1) , and the recoveries were in the range of 92.1-99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.
26681529	3	26	theme	hollow	660:665	arg1	fiber					667:671	a hollow fiber	658:671	a hollow fiber immersed in butyl ether	658:695	After derivatization, the sample was injected into the lumen of a hollow fiber immersed in butyl ether and separated by liquid chromatography.
26681529	1	27	theme	activity	274:281	arg1	determination					283:295	biological activity determination	263:295	biological activity determination	263:295	The monosaccharide compositions of functional polysaccharides are essential for structure elucidation and biological activity determination.
26681529	0	28	theme	polysaccharides	56:70	arg1	analysis					27:34	Monosaccharide composition analysis	0:34	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.	0:155	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.
26681529	5	29	theme	polysaccharides	1073:1087	arg1	composition					1050:1060	the monosaccharide composition	1031:1060	the monosaccharide composition of various polysaccharides	1031:1087	The limits of detection were in the range of 0.04-1.58 μmol L(-1) , and the recoveries were in the range of 92.1-99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.
26681529	2	30	theme	amino	516:520	arg1	sugars					522:527	two amino sugars	512:527	two amino sugars	512:527	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	0	31	theme	immunomodulatory	39:54	arg1	polysaccharides					56:70	immunomodulatory polysaccharides	39:70	immunomodulatory polysaccharides	39:70	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.
26681529	2	32	theme	immunomodulatory	560:575	arg1	polysaccharides					577:591	the immunomodulatory polysaccharides	556:591	the immunomodulatory polysaccharides	556:591	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	4	33	theme	optimized	743:751	arg1	conditions					753:762	optimized conditions	743:762	optimized conditions	743:762	Under optimized conditions, the calibration curves were linear (r ≥ 0.9996) in the range of 10-2000 μmol L(-1) .
26681529	0	34	theme	on-line	75:81	arg1	microextraction					96:110	on-line hollow fiber microextraction	75:110	on-line hollow fiber microextraction with high-performance liquid chromatography	75:154	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.
26681529	5	35	theme	monosaccharide	1035:1048	arg1	composition					1050:1060	the monosaccharide composition	1031:1060	the monosaccharide composition of various polysaccharides	1031:1087	The limits of detection were in the range of 0.04-1.58 μmol L(-1) , and the recoveries were in the range of 92.1-99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.
26681529	2	36	theme	sensitive	300:308	arg1	method					310:315	A sensitive method	298:315	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography	298:418	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	3	37	theme	butyl	685:689	arg1	ether					691:695	butyl ether	685:695	butyl ether	685:695	After derivatization, the sample was injected into the lumen of a hollow fiber immersed in butyl ether and separated by liquid chromatography.
26681529	1	38	theme	monosaccharide	161:174	arg1	compositions					176:187	The monosaccharide compositions	157:187	The monosaccharide compositions of functional polysaccharides	157:217	The monosaccharide compositions of functional polysaccharides are essential for structure elucidation and biological activity determination.
26681529	1	38	theme	monosaccharide	161:174	arg1	essential					223:231	essential	223:231	essential	223:231	The monosaccharide compositions of functional polysaccharides are essential for structure elucidation and biological activity determination.
26681529	3	39	theme	liquid	714:719	arg1	chromatography					721:734	liquid chromatography	714:734	liquid chromatography	714:734	After derivatization, the sample was injected into the lumen of a hollow fiber immersed in butyl ether and separated by liquid chromatography.
26681529	2	40	theme	liquid-phase	347:358	arg1	microextraction					360:374	on-line hollow-fiber liquid-phase microextraction	326:374	on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography	326:418	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	5	41	theme	composition	1050:1060	arg1	analysis					1019:1026	the analysis	1015:1026	the analysis of the monosaccharide composition of various polysaccharides	1015:1087	The limits of detection were in the range of 0.04-1.58 μmol L(-1) , and the recoveries were in the range of 92.1-99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.
26681529	2	42	theme	hollow-fiber	334:345	arg1	microextraction					360:374	on-line hollow-fiber liquid-phase microextraction	326:374	on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography	326:418	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	1	43	theme	polysaccharides	203:217	arg1	compositions					176:187	The monosaccharide compositions	157:187	The monosaccharide compositions of functional polysaccharides	157:217	The monosaccharide compositions of functional polysaccharides are essential for structure elucidation and biological activity determination.
26681529	1	43	theme	polysaccharides	203:217	arg1	essential					223:231	essential	223:231	essential	223:231	The monosaccharide compositions of functional polysaccharides are essential for structure elucidation and biological activity determination.
26681529	2	44	with	microextraction	360:374	arg1	chromatography					405:418	high-performance liquid chromatography	381:418	high-performance liquid chromatography	381:418	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	0	45	theme	fiber	90:94	arg1	microextraction					96:110	on-line hollow fiber microextraction	75:110	on-line hollow fiber microextraction with high-performance liquid chromatography	75:154	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.
26681529	2	46	theme	on-line	326:332	arg1	microextraction					360:374	on-line hollow-fiber liquid-phase microextraction	326:374	on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography	326:418	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
26681529	1	47	theme	functional	192:201	arg1	polysaccharides					203:217	functional polysaccharides	192:217	functional polysaccharides	192:217	The monosaccharide compositions of functional polysaccharides are essential for structure elucidation and biological activity determination.
26681529	5	48	theme	L	910:910	arg1	range					949:953	the range	945:953	the range	945:953	The limits of detection were in the range of 0.04-1.58 μmol L(-1) , and the recoveries were in the range of 92.1-99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.
26681529	5	48	theme	L	910:910	arg1	range					886:890	the range	882:890	the range of 0.04-1.58 μmol L(-1)	882:914	The limits of detection were in the range of 0.04-1.58 μmol L(-1) , and the recoveries were in the range of 92.1-99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.
26681529	0	49	theme	hollow	83:88	arg1	microextraction					96:110	on-line hollow fiber microextraction	75:110	on-line hollow fiber microextraction with high-performance liquid chromatography	75:154	Monosaccharide composition analysis of immunomodulatory polysaccharides by on-line hollow fiber microextraction with high-performance liquid chromatography.
26681529	2	50	theme	compositions	480:491	arg1	analysis					449:456	the analysis	445:456	the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides	445:591	A sensitive method based on on-line hollow-fiber liquid-phase microextraction with high-performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides.
24398151	5	0	theme	carbon	655:660	arg1	content					662:668	organic carbon content	647:668	organic carbon content of the initial process water	647:697	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	6	1	theme	product	930:936	arg1	composition					938:948	time-dependent carbonization product composition	901:948	time-dependent carbonization product composition	901:948	Results from the experiments conducted evaluating the influence of process water quality on carbonization indicate that changes in initial water quality do influence time-dependent carbonization product composition and yields.
24398151	1	2	theme	feedstocks	243:252	arg1	conversion					225:234	the conversion	221:234	the conversion of wet feedstocks	221:252	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24398151	4	3	theme	alternative	516:526	arg1	candidates					542:551	ideal alternative liquid source candidates	510:551	ideal alternative liquid source candidates	510:551	Liquid waste streams may be ideal alternative liquid source candidates.
24398151	6	4	theme	initial	866:872	arg1	quality					880:886	initial water quality	866:886	initial water quality	866:886	Results from the experiments conducted evaluating the influence of process water quality on carbonization indicate that changes in initial water quality do influence time-dependent carbonization product composition and yields.
24398151	6	5	from	experiments	752:762	arg1	Results					735:741	Results	735:741	Results from the experiments conducted evaluating the influence of process water quality on carbonization	735:839	Results from the experiments conducted evaluating the influence of process water quality on carbonization indicate that changes in initial water quality do influence time-dependent carbonization product composition and yields.
24398151	7	6	from	influence	1118:1126	arg1	products/yields					1151:1165	final carbonization products/yields	1131:1165	final carbonization products/yields	1131:1165	These results also suggest that using municipal and industrial wastewaters, with the exception of streams with high CaCl2 concentrations, may impart little influence on final carbonization products/yields.
24398151	0	7	from	Influence	0:8	arg1	carbonization					51:63	hydrothermal carbonization	38:63	hydrothermal carbonization of cellulose	38:76	Influence of process water quality on hydrothermal carbonization of cellulose.
24398151	4	8	theme	Liquid	482:487	arg1	streams					495:501	Liquid waste streams	482:501	Liquid waste streams	482:501	Liquid waste streams may be ideal alternative liquid source candidates.
24398151	6	9	from	influence	789:797	arg1	carbonization					827:839	carbonization	827:839	carbonization	827:839	Results from the experiments conducted evaluating the influence of process water quality on carbonization indicate that changes in initial water quality do influence time-dependent carbonization product composition and yields.
24398151	5	10	theme	ionic	627:631	arg1	strength					633:640	ionic strength	627:640	ionic strength	627:640	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	7	11	dep	impart	1104:1109	arg1	using					994:998	using	994:998	using municipal and industrial wastewaters	994:1035	These results also suggest that using municipal and industrial wastewaters, with the exception of streams with high CaCl2 concentrations, may impart little influence on final carbonization products/yields.
24398151	7	12	with	streams	1060:1066	arg1	concentrations					1084:1097	high CaCl2 concentrations	1073:1097	high CaCl2 concentrations	1073:1097	These results also suggest that using municipal and industrial wastewaters, with the exception of streams with high CaCl2 concentrations, may impart little influence on final carbonization products/yields.
24398151	1	13	theme	Hydrothermal	79:90	arg1	advantageous					204:215	advantageous	204:215	advantageous	204:215	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24398151	1	13	theme	Hydrothermal	79:90	arg1	HTC					107:109	HTC	107:109	HTC	107:109	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24398151	1	13	theme	Hydrothermal	79:90	arg1	process					136:142	a thermal conversion process	115:142	a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks	115:252	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24398151	1	13	theme	Hydrothermal	79:90	arg1	carbonization					92:104	Hydrothermal carbonization	79:104	Hydrothermal carbonization (HTC)	79:110	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24398151	4	14	theme	waste	489:493	arg1	streams					495:501	Liquid waste streams	482:501	Liquid waste streams	482:501	Liquid waste streams may be ideal alternative liquid source candidates.
24398151	7	15	theme	carbonization	1137:1149	arg1	products/yields					1151:1165	final carbonization products/yields	1131:1165	final carbonization products/yields	1131:1165	These results also suggest that using municipal and industrial wastewaters, with the exception of streams with high CaCl2 concentrations, may impart little influence on final carbonization products/yields.
24398151	6	16	theme	quality	816:822	arg1	influence					789:797	the influence	785:797	the influence of process water quality on carbonization	785:839	Results from the experiments conducted evaluating the influence of process water quality on carbonization indicate that changes in initial water quality do influence time-dependent carbonization product composition and yields.
24398151	2	17	theme	water	306:310	arg1	form					293:296	the form	289:296	the form of pure water	289:310	Supplemental moisture, usually in the form of pure water, is added during carbonization to achieve feedstock submersion.
24398151	6	18	from	changes	855:861	arg1	quality					880:886	initial water quality	866:886	initial water quality	866:886	Results from the experiments conducted evaluating the influence of process water quality on carbonization indicate that changes in initial water quality do influence time-dependent carbonization product composition and yields.
24398151	5	19	theme	organic	647:653	arg1	content					662:668	organic carbon content	647:668	organic carbon content of the initial process water	647:697	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	6	20	theme	water	810:814	arg1	quality					816:822	process water quality	802:822	process water quality	802:822	Results from the experiments conducted evaluating the influence of process water quality on carbonization indicate that changes in initial water quality do influence time-dependent carbonization product composition and yields.
24398151	2	21	theme	pure	301:304	arg1	water					306:310	pure water	301:310	pure water	301:310	Supplemental moisture, usually in the form of pure water, is added during carbonization to achieve feedstock submersion.
24398151	0	22	theme	water	21:25	arg1	quality					27:33	process water quality	13:33	process water quality	13:33	Influence of process water quality on hydrothermal carbonization of cellulose.
24398151	5	23	theme	cellulose	710:718	arg1	carbonization					720:732	cellulose carbonization	710:732	cellulose carbonization	710:732	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	6	24	theme	process	802:808	arg1	quality					816:822	process water quality	802:822	process water quality	802:822	Results from the experiments conducted evaluating the influence of process water quality on carbonization indicate that changes in initial water quality do influence time-dependent carbonization product composition and yields.
24398151	3	25	theme	moisture	464:471	arg1	sources					473:479	alternative supplemental moisture sources	439:479	alternative supplemental moisture sources	439:479	To improve process sustainability, it is important to consider alternative supplemental moisture sources.
24398151	0	26	theme	process	13:19	arg1	quality					27:33	process water quality	13:33	process water quality	13:33	Influence of process water quality on hydrothermal carbonization of cellulose.
24398151	7	27	theme	final	1131:1135	arg1	products/yields					1151:1165	final carbonization products/yields	1131:1165	final carbonization products/yields	1131:1165	These results also suggest that using municipal and industrial wastewaters, with the exception of streams with high CaCl2 concentrations, may impart little influence on final carbonization products/yields.
24398151	6	28	theme	carbonization	916:928	arg1	composition					938:948	time-dependent carbonization product composition	901:948	time-dependent carbonization product composition	901:948	Results from the experiments conducted evaluating the influence of process water quality on carbonization indicate that changes in initial water quality do influence time-dependent carbonization product composition and yields.
24398151	2	29	from	moisture	268:275	arg1	form					293:296	the form	289:296	the form of pure water	289:310	Supplemental moisture, usually in the form of pure water, is added during carbonization to achieve feedstock submersion.
24398151	7	30	theme	little	1111:1116	arg1	influence					1118:1126	little influence	1111:1126	little influence on final carbonization products/yields	1111:1165	These results also suggest that using municipal and industrial wastewaters, with the exception of streams with high CaCl2 concentrations, may impart little influence on final carbonization products/yields.
24398151	6	31	theme	time-dependent	901:914	arg1	composition					938:948	time-dependent carbonization product composition	901:948	time-dependent carbonization product composition	901:948	Results from the experiments conducted evaluating the influence of process water quality on carbonization indicate that changes in initial water quality do influence time-dependent carbonization product composition and yields.
24398151	3	32	theme	alternative	439:449	arg1	sources					473:479	alternative supplemental moisture sources	439:479	alternative supplemental moisture sources	439:479	To improve process sustainability, it is important to consider alternative supplemental moisture sources.
24398151	0	33	theme	quality	27:33	arg1	Influence					0:8	Influence	0:8	Influence of process water quality on hydrothermal carbonization of cellulose.	0:77	Influence of process water quality on hydrothermal carbonization of cellulose.
24398151	4	34	theme	source	535:540	arg1	candidates					542:551	ideal alternative liquid source candidates	510:551	ideal alternative liquid source candidates	510:551	Liquid waste streams may be ideal alternative liquid source candidates.
24398151	7	35	theme	high	1073:1076	arg1	concentrations					1084:1097	high CaCl2 concentrations	1073:1097	high CaCl2 concentrations	1073:1097	These results also suggest that using municipal and industrial wastewaters, with the exception of streams with high CaCl2 concentrations, may impart little influence on final carbonization products/yields.
24398151	3	36	theme	supplemental	451:462	arg1	sources					473:479	alternative supplemental moisture sources	439:479	alternative supplemental moisture sources	439:479	To improve process sustainability, it is important to consider alternative supplemental moisture sources.
24398151	3	37	theme	process	387:393	arg1	sustainability					395:408	process sustainability	387:408	process sustainability	387:408	To improve process sustainability, it is important to consider alternative supplemental moisture sources.
24398151	4	38	theme	liquid	528:533	arg1	candidates					542:551	ideal alternative liquid source candidates	510:551	ideal alternative liquid source candidates	510:551	Liquid waste streams may be ideal alternative liquid source candidates.
24398151	5	39	theme	initial	677:683	arg1	water					693:697	the initial process water	673:697	the initial process water	673:697	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	7	40	theme	CaCl2	1078:1082	arg1	concentrations					1084:1097	high CaCl2 concentrations	1073:1097	high CaCl2 concentrations	1073:1097	These results also suggest that using municipal and industrial wastewaters, with the exception of streams with high CaCl2 concentrations, may impart little influence on final carbonization products/yields.
24398151	0	41	theme	hydrothermal	38:49	arg1	carbonization					51:63	hydrothermal carbonization	38:63	hydrothermal carbonization of cellulose	38:76	Influence of process water quality on hydrothermal carbonization of cellulose.
24398151	5	42	theme	process	685:691	arg1	water					693:697	the initial process water	673:697	the initial process water	673:697	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	7	43	theme	streams	1060:1066	arg1	exception					1047:1055	the exception	1043:1055	the exception of streams with high CaCl2 concentrations	1043:1097	These results also suggest that using municipal and industrial wastewaters, with the exception of streams with high CaCl2 concentrations, may impart little influence on final carbonization products/yields.
24398151	5	44	from	changes	612:618	arg1	content					662:668	organic carbon content	647:668	organic carbon content of the initial process water	647:697	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	5	44	from	changes	612:618	arg1	strength					633:640	ionic strength	627:640	ionic strength	627:640	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	5	44	from	changes	612:618	arg1	pH					623:624	pH	623:624	pH	623:624	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	5	45	theme	water	693:697	arg1	pH					623:624	pH	623:624	pH	623:624	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	5	45	theme	water	693:697	arg1	strength					633:640	ionic strength	627:640	ionic strength	627:640	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	5	45	theme	water	693:697	arg1	content					662:668	organic carbon content	647:668	organic carbon content of the initial process water	647:697	Experiments were conducted to systematically evaluate how changes in pH, ionic strength, and organic carbon content of the initial process water influences cellulose carbonization.
24398151	6	46	theme	water	874:878	arg1	quality					880:886	initial water quality	866:886	initial water quality	866:886	Results from the experiments conducted evaluating the influence of process water quality on carbonization indicate that changes in initial water quality do influence time-dependent carbonization product composition and yields.
24398151	1	47	theme	thermal	117:123	arg1	advantageous					204:215	advantageous	204:215	advantageous	204:215	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24398151	1	47	theme	thermal	117:123	arg1	carbonization					92:104	Hydrothermal carbonization	79:104	Hydrothermal carbonization (HTC)	79:110	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24398151	1	47	theme	thermal	117:123	arg1	process					136:142	a thermal conversion process	115:142	a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks	115:252	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24398151	4	48	theme	ideal	510:514	arg1	candidates					542:551	ideal alternative liquid source candidates	510:551	ideal alternative liquid source candidates	510:551	Liquid waste streams may be ideal alternative liquid source candidates.
24398151	2	49	theme	Supplemental	255:266	arg1	moisture					268:275	Supplemental moisture	255:275	Supplemental moisture	255:275	Supplemental moisture, usually in the form of pure water, is added during carbonization to achieve feedstock submersion.
24398151	1	50	theme	conversion	125:134	arg1	advantageous					204:215	advantageous	204:215	advantageous	204:215	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24398151	1	50	theme	conversion	125:134	arg1	carbonization					92:104	Hydrothermal carbonization	79:104	Hydrothermal carbonization (HTC)	79:110	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24398151	1	50	theme	conversion	125:134	arg1	process					136:142	a thermal conversion process	115:142	a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks	115:252	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24398151	0	51	theme	cellulose	68:76	arg1	carbonization					51:63	hydrothermal carbonization	38:63	hydrothermal carbonization of cellulose	38:76	Influence of process water quality on hydrothermal carbonization of cellulose.
24398151	2	52	theme	feedstock	354:362	arg1	submersion					364:373	feedstock submersion	354:373	feedstock submersion	354:373	Supplemental moisture, usually in the form of pure water, is added during carbonization to achieve feedstock submersion.
24398151	7	53	theme	industrial	1014:1023	arg1	wastewaters					1025:1035	municipal and industrial wastewaters	1000:1035	municipal and industrial wastewaters	1000:1035	These results also suggest that using municipal and industrial wastewaters, with the exception of streams with high CaCl2 concentrations, may impart little influence on final carbonization products/yields.
24398151	7	54	theme	municipal	1000:1008	arg1	wastewaters					1025:1035	municipal and industrial wastewaters	1000:1035	municipal and industrial wastewaters	1000:1035	These results also suggest that using municipal and industrial wastewaters, with the exception of streams with high CaCl2 concentrations, may impart little influence on final carbonization products/yields.
24398151	1	55	theme	wet	239:241	arg1	feedstocks					243:252	wet feedstocks	239:252	wet feedstocks	239:252	Hydrothermal carbonization (HTC) is a thermal conversion process that has been shown to be environmentally and energetically advantageous for the conversion of wet feedstocks.
24400918	2	0	theme	maximum	457:463	arg1	ϕw					488:489	ϕw	488:489	ϕw	488:489	When using freeze-dried C6-BCN and C12-BCN, only a maximum water volume fraction (ϕw) of 60% could be stabilized, while no emulsion was obtained for C2-BCN.
24400918	2	0	theme	maximum	457:463	arg1	fraction					478:485	only a maximum water volume fraction	450:485	only a maximum water volume fraction (ϕw) of 60%	450:497	When using freeze-dried C6-BCN and C12-BCN, only a maximum water volume fraction (ϕw) of 60% could be stabilized, while no emulsion was obtained for C2-BCN.
24400918	4	1	theme	freeze-dried	883:894	arg1	bundles					909:915	freeze-dried modified BCN bundles	883:915	freeze-dried modified BCN bundles	883:915	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	4	2	with	consistent	829:838	arg1	dispersion					869:878	dispersion	869:878	dispersion	869:878	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	4	2	with	consistent	829:838	arg1	disentanglement					849:863	disentanglement	849:863	disentanglement	849:863	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	1	3	theme	organic	271:277	arg1	acids					279:283	organic acids	271:283	organic acids of various chain lengths	271:308	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	1	4	theme	bacterial	184:192	arg1	BCNs					216:219	BCNs	216:219	BCNs	216:219	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	1	4	theme	bacterial	184:192	arg1	nanofibers					204:213	bacterial cellulose nanofibers	184:213	bacterial cellulose nanofibers (BCNs)	184:220	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	3	5	theme	phase	736:740	arg1	emulsions					759:767	high internal phase water-in-toluene emulsions	722:767	high internal phase water-in-toluene emulsions	722:767	However, the maximum ϕw increased to 71%, 81%, and 77% for C2-BCN, C6-BCN, and C12-BCN, respectively, 150 h after the initial emulsification, thereby creating high internal phase water-in-toluene emulsions.
24400918	5	6	theme	catastrophic	981:992	arg1	separation					1000:1009	catastrophic phase separation	981:1009	catastrophic phase separation	981:1009	These emulsions exhibited catastrophic phase separation when ϕw was increased, as opposed to catastrophic phase inversion observed for other Pickering emulsions.
24400918	0	7	theme	hydrophobized	93:105	arg1	nanofibrils					127:137	hydrophobized bacterial cellulose nanofibrils	93:137	hydrophobized bacterial cellulose nanofibrils	93:137	Phase behavior of medium and high internal phase water-in-oil emulsions stabilized solely by hydrophobized bacterial cellulose nanofibrils.
24400918	5	8	theme	catastrophic	1048:1059	arg1	inversion					1067:1075	catastrophic phase inversion	1048:1075	catastrophic phase inversion observed for other Pickering emulsions	1048:1114	These emulsions exhibited catastrophic phase separation when ϕw was increased, as opposed to catastrophic phase inversion observed for other Pickering emulsions.
24400918	5	9	theme	Pickering	1096:1104	arg1	emulsions					1106:1114	other Pickering emulsions	1090:1114	other Pickering emulsions	1090:1114	These emulsions exhibited catastrophic phase separation when ϕw was increased, as opposed to catastrophic phase inversion observed for other Pickering emulsions.
24400918	3	10	theme	water-in-toluene	742:757	arg1	emulsions					759:767	high internal phase water-in-toluene emulsions	722:767	high internal phase water-in-toluene emulsions	722:767	However, the maximum ϕw increased to 71%, 81%, and 77% for C2-BCN, C6-BCN, and C12-BCN, respectively, 150 h after the initial emulsification, thereby creating high internal phase water-in-toluene emulsions.
24400918	4	11	with	nanofibers	933:942	arg1	time					949:952	time	949:952	time	949:952	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	1	12	theme	various	288:294	arg1	lengths					302:308	various chain lengths	288:308	various chain lengths	288:308	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	0	13	theme	cellulose	117:125	arg1	nanofibrils					127:137	hydrophobized bacterial cellulose nanofibrils	93:137	hydrophobized bacterial cellulose nanofibrils	93:137	Phase behavior of medium and high internal phase water-in-oil emulsions stabilized solely by hydrophobized bacterial cellulose nanofibrils.
24400918	1	14	theme	dodecanoic	349:358	arg1	acid					360:363	dodecanoic acid	349:363	dodecanoic acid	349:363	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	1	15	theme	cellulose	194:202	arg1	BCNs					216:219	BCNs	216:219	BCNs	216:219	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	1	15	theme	cellulose	194:202	arg1	nanofibers					204:213	bacterial cellulose nanofibers	184:213	bacterial cellulose nanofibers (BCNs)	184:220	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	0	16	theme	bacterial	107:115	arg1	nanofibrils					127:137	hydrophobized bacterial cellulose nanofibrils	93:137	hydrophobized bacterial cellulose nanofibrils	93:137	Phase behavior of medium and high internal phase water-in-oil emulsions stabilized solely by hydrophobized bacterial cellulose nanofibrils.
24400918	1	17	dep	acid	318:321	arg1	C2-					324:326	C2-	324:326	C2-	324:326	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	1	17	dep	acid	318:321	arg1	C12-					366:369	C12-	366:369	C12-	366:369	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	1	17	dep	acid	318:321	arg1	C6-					344:346	C6-	344:346	C6-	344:346	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	1	17	dep	acid	318:321	arg1	acid					360:363	dodecanoic acid	349:363	dodecanoic acid	349:363	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	1	17	dep	acid	318:321	arg1	acid					338:341	hexanoic acid	329:341	hexanoic acid	329:341	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	4	18	theme	BCN	905:907	arg1	bundles					909:915	freeze-dried modified BCN bundles	883:915	freeze-dried modified BCN bundles	883:915	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	2	19	theme	freeze-dried	417:428	arg1	C6-BCN					430:435	freeze-dried C6-BCN	417:435	freeze-dried C6-BCN	417:435	When using freeze-dried C6-BCN and C12-BCN, only a maximum water volume fraction (ϕw) of 60% could be stabilized, while no emulsion was obtained for C2-BCN.
24400918	2	20	theme	volume	471:476	arg1	ϕw					488:489	ϕw	488:489	ϕw	488:489	When using freeze-dried C6-BCN and C12-BCN, only a maximum water volume fraction (ϕw) of 60% could be stabilized, while no emulsion was obtained for C2-BCN.
24400918	2	20	theme	volume	471:476	arg1	fraction					478:485	only a maximum water volume fraction	450:485	only a maximum water volume fraction (ϕw) of 60%	450:497	When using freeze-dried C6-BCN and C12-BCN, only a maximum water volume fraction (ϕw) of 60% could be stabilized, while no emulsion was obtained for C2-BCN.
24400918	0	21	theme	Phase	0:4	arg1	behavior					6:13	Phase behavior	0:13	Phase behavior of medium and high internal phase water-in-oil emulsions	0:70	Phase behavior of medium and high internal phase water-in-oil emulsions stabilized solely by hydrophobized bacterial cellulose nanofibrils.
24400918	1	22	theme	chain	296:300	arg1	lengths					302:308	various chain lengths	288:308	various chain lengths	288:308	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	4	23	theme	individual	922:931	arg1	nanofibers					933:942	individual nanofibers	922:942	individual nanofibers with time	922:952	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	0	24	theme	medium	18:23	arg1	behavior					6:13	Phase behavior	0:13	Phase behavior of medium and high internal phase water-in-oil emulsions	0:70	Phase behavior of medium and high internal phase water-in-oil emulsions stabilized solely by hydrophobized bacterial cellulose nanofibrils.
24400918	1	25	theme	lengths	302:308	arg1	acids					279:283	organic acids	271:283	organic acids of various chain lengths	271:308	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	4	26	dep	disentanglement	849:863	arg1	the					845:847	the	845:847	the	845:847	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	3	27	theme	initial	681:687	arg1	emulsification					689:702	the initial emulsification	677:702	the initial emulsification	677:702	However, the maximum ϕw increased to 71%, 81%, and 77% for C2-BCN, C6-BCN, and C12-BCN, respectively, 150 h after the initial emulsification, thereby creating high internal phase water-in-toluene emulsions.
24400918	2	28	theme	%	497:497	arg1	ϕw					488:489	ϕw	488:489	ϕw	488:489	When using freeze-dried C6-BCN and C12-BCN, only a maximum water volume fraction (ϕw) of 60% could be stabilized, while no emulsion was obtained for C2-BCN.
24400918	2	28	theme	%	497:497	arg1	fraction					478:485	only a maximum water volume fraction	450:485	only a maximum water volume fraction (ϕw) of 60%	450:497	When using freeze-dried C6-BCN and C12-BCN, only a maximum water volume fraction (ϕw) of 60% could be stabilized, while no emulsion was obtained for C2-BCN.
24400918	3	29	theme	maximum	576:582	arg1	ϕw					584:585	the maximum ϕw	572:585	the maximum ϕw	572:585	However, the maximum ϕw increased to 71%, 81%, and 77% for C2-BCN, C6-BCN, and C12-BCN, respectively, 150 h after the initial emulsification, thereby creating high internal phase water-in-toluene emulsions.
24400918	0	30	theme	high	29:32	arg1	emulsions					62:70	high internal phase water-in-oil emulsions	29:70	high internal phase water-in-oil emulsions	29:70	Phase behavior of medium and high internal phase water-in-oil emulsions stabilized solely by hydrophobized bacterial cellulose nanofibrils.
24400918	1	31	theme	acetic	311:316	arg1	acid					318:321	acetic acid	311:321	acetic acid	311:321	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	5	32	theme	phase	994:998	arg1	separation					1000:1009	catastrophic phase separation	981:1009	catastrophic phase separation	981:1009	These emulsions exhibited catastrophic phase separation when ϕw was increased, as opposed to catastrophic phase inversion observed for other Pickering emulsions.
24400918	4	33	theme	modified	896:903	arg1	bundles					909:915	freeze-dried modified BCN bundles	883:915	freeze-dried modified BCN bundles	883:915	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	4	34	theme	bundles	909:915	arg1	dispersion					869:878	dispersion	869:878	dispersion	869:878	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	4	34	theme	bundles	909:915	arg1	disentanglement					849:863	disentanglement	849:863	disentanglement	849:863	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	4	35	theme	emulsions	816:824	arg1	consistent					829:838	consistent	829:838	consistent	829:838	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	4	35	theme	emulsions	816:824	arg1	behavior					798:805	The observed time-dependent behavior	770:805	The observed time-dependent behavior of these emulsions	770:824	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	1	36	dep	hydrophobized	234:246	arg1	acid					318:321	acetic acid	311:321	acetic acid	311:321	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	0	37	theme	phase	43:47	arg1	emulsions					62:70	high internal phase water-in-oil emulsions	29:70	high internal phase water-in-oil emulsions	29:70	Phase behavior of medium and high internal phase water-in-oil emulsions stabilized solely by hydrophobized bacterial cellulose nanofibrils.
24400918	5	38	theme	phase	1061:1065	arg1	inversion					1067:1075	catastrophic phase inversion	1048:1075	catastrophic phase inversion observed for other Pickering emulsions	1048:1114	These emulsions exhibited catastrophic phase separation when ϕw was increased, as opposed to catastrophic phase inversion observed for other Pickering emulsions.
24400918	4	39	theme	observed	774:781	arg1	consistent					829:838	consistent	829:838	consistent	829:838	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	4	39	theme	observed	774:781	arg1	behavior					798:805	The observed time-dependent behavior	770:805	The observed time-dependent behavior of these emulsions	770:824	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	2	40	theme	water	465:469	arg1	ϕw					488:489	ϕw	488:489	ϕw	488:489	When using freeze-dried C6-BCN and C12-BCN, only a maximum water volume fraction (ϕw) of 60% could be stabilized, while no emulsion was obtained for C2-BCN.
24400918	2	40	theme	water	465:469	arg1	fraction					478:485	only a maximum water volume fraction	450:485	only a maximum water volume fraction (ϕw) of 60%	450:497	When using freeze-dried C6-BCN and C12-BCN, only a maximum water volume fraction (ϕw) of 60% could be stabilized, while no emulsion was obtained for C2-BCN.
24400918	0	41	theme	internal	34:41	arg1	emulsions					62:70	high internal phase water-in-oil emulsions	29:70	high internal phase water-in-oil emulsions	29:70	Phase behavior of medium and high internal phase water-in-oil emulsions stabilized solely by hydrophobized bacterial cellulose nanofibrils.
24400918	3	42	theme	high	722:725	arg1	emulsions					759:767	high internal phase water-in-toluene emulsions	722:767	high internal phase water-in-toluene emulsions	722:767	However, the maximum ϕw increased to 71%, 81%, and 77% for C2-BCN, C6-BCN, and C12-BCN, respectively, 150 h after the initial emulsification, thereby creating high internal phase water-in-toluene emulsions.
24400918	1	43	theme	Water-in-oil	140:151	arg1	emulsions					153:161	Water-in-oil emulsions	140:161	Water-in-oil emulsions	140:161	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24400918	3	44	theme	internal	727:734	arg1	emulsions					759:767	high internal phase water-in-toluene emulsions	722:767	high internal phase water-in-toluene emulsions	722:767	However, the maximum ϕw increased to 71%, 81%, and 77% for C2-BCN, C6-BCN, and C12-BCN, respectively, 150 h after the initial emulsification, thereby creating high internal phase water-in-toluene emulsions.
24400918	4	45	theme	time-dependent	783:796	arg1	consistent					829:838	consistent	829:838	consistent	829:838	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	4	45	theme	time-dependent	783:796	arg1	behavior					798:805	The observed time-dependent behavior	770:805	The observed time-dependent behavior of these emulsions	770:824	The observed time-dependent behavior of these emulsions is consistent with the disentanglement and dispersion of freeze-dried modified BCN bundles into individual nanofibers with time.
24400918	0	46	theme	emulsions	62:70	arg1	behavior					6:13	Phase behavior	0:13	Phase behavior of medium and high internal phase water-in-oil emulsions	0:70	Phase behavior of medium and high internal phase water-in-oil emulsions stabilized solely by hydrophobized bacterial cellulose nanofibrils.
24400918	5	47	theme	other	1090:1094	arg1	emulsions					1106:1114	other Pickering emulsions	1090:1114	other Pickering emulsions	1090:1114	These emulsions exhibited catastrophic phase separation when ϕw was increased, as opposed to catastrophic phase inversion observed for other Pickering emulsions.
24400918	0	48	theme	water-in-oil	49:60	arg1	emulsions					62:70	high internal phase water-in-oil emulsions	29:70	high internal phase water-in-oil emulsions	29:70	Phase behavior of medium and high internal phase water-in-oil emulsions stabilized solely by hydrophobized bacterial cellulose nanofibrils.
24400918	1	49	theme	hexanoic	329:336	arg1	acid					338:341	hexanoic acid	329:341	hexanoic acid	329:341	Water-in-oil emulsions stabilized solely by bacterial cellulose nanofibers (BCNs), which were hydrophobized by esterification with organic acids of various chain lengths (acetic acid, C2-; hexanoic acid, C6-; dodecanoic acid, C12-), were produced and characterized.
24839262	0	0	theme	tissue	81:86	arg1	region					88:93	tissue region	81:93	tissue region	81:93	FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age.
24839262	8	1	from	cartilage	1464:1472	arg1	degree					1401:1406	a higher degree	1392:1406	a higher degree of collagen alignment in both the surface and deep zone cartilage	1392:1472	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	8	2	dep	increased	1366:1374	arg1	accompanied					1377:1387	accompanied	1377:1387	accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage	1377:1472	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	3	3	theme	quantitative	497:508	arg1	understanding					510:522	quantitative understanding	497:522	quantitative understanding of the matrix	497:536	Presently, there is a lack of quantitative understanding of the matrix and mineral distribution across this multitissue transition.
24839262	8	4	from	degree	1401:1406	arg1	surface					1442:1448	the surface	1438:1448	the surface	1438:1448	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	8	4	from	degree	1401:1406	arg1	cartilage					1464:1472	deep zone cartilage	1454:1472	deep zone cartilage	1454:1472	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	5	5	theme	cartilage-to-bone	774:790	arg1	transition					792:801	the cartilage-to-bone transition	770:801	the cartilage-to-bone transition	770:801	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	5	5	theme	cartilage-to-bone	774:790	arg1	function					808:815	a function	806:815	a function of age	806:822	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	10	6	theme	critical	1730:1737	arg1	parameters					1749:1758	critical benchmark parameters	1730:1758	critical benchmark parameters for current efforts in integrative cartilage repair	1730:1810	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
24839262	10	6	theme	critical	1730:1737	arg1	observations					1597:1608	These observations	1591:1608	These observations	1591:1608	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
24839262	3	7	theme	understanding	510:522	arg1	lack					489:492	a lack	487:492	a lack of quantitative understanding of the matrix	487:536	Presently, there is a lack of quantitative understanding of the matrix and mineral distribution across this multitissue transition.
24839262	3	7	theme	understanding	510:522	arg1	distribution					550:561	mineral distribution	542:561	mineral distribution across this multitissue transition	542:596	Presently, there is a lack of quantitative understanding of the matrix and mineral distribution across this multitissue transition.
24839262	6	8	theme	physiological	1113:1125	arg1	loading					1127:1133	postnatal physiological loading	1103:1133	postnatal physiological loading	1103:1133	Both tissue-dependent and age-related changes were observed, underscoring the role of postnatal physiological loading in matrix remodeling.
24839262	3	9	theme	mineral	542:548	arg1	distribution					550:561	mineral distribution	542:561	mineral distribution across this multitissue transition	542:596	Presently, there is a lack of quantitative understanding of the matrix and mineral distribution across this multitissue transition.
24839262	5	10	theme	mineral	961:967	arg1	distribution					969:980	mineral distribution	961:980	mineral distribution	961:980	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	1	11	theme	musculoskeletal	252:266	arg1	function					268:275	musculoskeletal function	252:275	musculoskeletal function	252:275	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	0	12	theme	region	88:93	arg1	function					69:76	a function	67:76	a function of tissue region and age	67:101	FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age.
24839262	10	13	from	efforts	1772:1778	arg1	repair					1805:1810	integrative cartilage repair	1783:1810	integrative cartilage repair	1783:1810	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
24839262	2	14	theme	osteochondral	282:294	arg1	interface					296:304	The osteochondral interface	278:304	The osteochondral interface	278:304	The osteochondral interface enables pressurization of articular cartilage, facilitates load transfer between cartilage and bone, and serves as a barrier between these two distinct tissues.
24839262	2	14	theme	osteochondral	282:294	arg1	barrier					423:429	a barrier	421:429	a barrier between these two distinct tissues	421:464	The osteochondral interface enables pressurization of articular cartilage, facilitates load transfer between cartilage and bone, and serves as a barrier between these two distinct tissues.
24839262	5	15	from	organization	1003:1014	arg1	proteoglycan					943:954	proteoglycan	943:954	proteoglycan	943:954	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	5	15	from	organization	1003:1014	arg1	collagen					933:940	collagen	933:940	collagen	933:940	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	5	15	from	organization	1003:1014	arg1	distribution					969:980	mineral distribution	961:980	mineral distribution	961:980	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	6	16	theme	matrix	1138:1143	arg1	remodeling					1145:1154	matrix remodeling	1138:1154	matrix remodeling	1138:1154	Both tissue-dependent and age-related changes were observed, underscoring the role of postnatal physiological loading in matrix remodeling.
24839262	5	17	theme	study	745:749	arg1	objective					727:735	the objective	723:735	the objective of this study	723:749	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	1	18	theme	Soft	104:107	arg1	transitions					124:134	Soft tissue-to-bone transitions	104:134	Soft tissue-to-bone transitions	104:134	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	1	18	theme	Soft	104:107	arg1	interface					163:171	the osteochondral interface	145:171	the osteochondral interface	145:171	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	1	18	theme	Soft	104:107	arg1	junctions					186:194	complex junctions	178:194	complex junctions that connect multiple tissue types and are critical for musculoskeletal function	178:275	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	6	19	from	role	1095:1098	arg1	remodeling					1145:1154	matrix remodeling	1138:1154	matrix remodeling	1138:1154	Both tissue-dependent and age-related changes were observed, underscoring the role of postnatal physiological loading in matrix remodeling.
24839262	3	20	theme	matrix	531:536	arg1	understanding					510:522	quantitative understanding	497:522	quantitative understanding of the matrix	497:536	Presently, there is a lack of quantitative understanding of the matrix and mineral distribution across this multitissue transition.
24839262	4	21	theme	maturity	673:680	arg1	onset					655:659	the onset	651:659	the onset of skeletal maturity	651:680	Moreover, age-related changes at the interface with the onset of skeletal maturity are also not well understood.
24839262	0	22	theme	age	99:101	arg1	function					69:76	a function	67:76	a function of tissue region and age	67:101	FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age.
24839262	5	23	theme	region-dependent	905:920	arg1	changes					922:928	region-dependent changes	905:928	region-dependent changes in collagen, proteoglycan, and mineral distribution	905:980	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	0	24	theme	FTIR-I	0:5	arg1	mapping					21:27	FTIR-I compositional mapping	0:27	FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age.	0:102	FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age.
24839262	6	25	theme	postnatal	1103:1111	arg1	loading					1127:1133	postnatal physiological loading	1103:1133	postnatal physiological loading	1103:1133	Both tissue-dependent and age-related changes were observed, underscoring the role of postnatal physiological loading in matrix remodeling.
24839262	4	26	theme	skeletal	664:671	arg1	maturity					673:680	skeletal maturity	664:680	skeletal maturity	664:680	Moreover, age-related changes at the interface with the onset of skeletal maturity are also not well understood.
24839262	0	27	theme	compositional	7:19	arg1	mapping					21:27	FTIR-I compositional mapping	0:27	FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age.	0:102	FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age.
24839262	10	28	theme	region-	1640:1646	arg1	changes					1666:1672	both region- and age-dependent changes	1635:1672	both region- and age-dependent changes across the cartilage-to-bone junction	1635:1710	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
24839262	7	29	theme	deep	1294:1297	arg1	zone					1299:1302	the deep zone	1290:1302	the deep zone of cartilage	1290:1315	It was observed that the relative collagen content increased continuously from cartilage to bone, whereas proteoglycan peaked within the deep zone of cartilage.
24839262	2	30	theme	articular	332:340	arg1	cartilage					342:350	articular cartilage	332:350	articular cartilage	332:350	The osteochondral interface enables pressurization of articular cartilage, facilitates load transfer between cartilage and bone, and serves as a barrier between these two distinct tissues.
24839262	6	31	theme	age-related	1043:1053	arg1	changes					1055:1061	Both tissue-dependent and age-related changes	1017:1061	Both tissue-dependent and age-related changes	1017:1061	Both tissue-dependent and age-related changes were observed, underscoring the role of postnatal physiological loading in matrix remodeling.
24839262	5	32	theme	FTIR-I	881:886	arg1	analysis					889:896	spectroscopic imaging (FTIR-I) analysis	858:896	spectroscopic imaging (FTIR-I) analysis	858:896	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	2	33	theme	distinct	449:456	arg1	tissues					458:464	these two distinct tissues	439:464	these two distinct tissues	439:464	The osteochondral interface enables pressurization of articular cartilage, facilitates load transfer between cartilage and bone, and serves as a barrier between these two distinct tissues.
24839262	10	34	theme	age-dependent	1652:1664	arg1	changes					1666:1672	both region- and age-dependent changes	1635:1672	both region- and age-dependent changes across the cartilage-to-bone junction	1635:1710	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
24839262	5	35	from	changes	922:928	arg1	proteoglycan					943:954	proteoglycan	943:954	proteoglycan	943:954	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	5	35	from	changes	922:928	arg1	collagen					933:940	collagen	933:940	collagen	933:940	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	5	35	from	changes	922:928	arg1	distribution					969:980	mineral distribution	961:980	mineral distribution	961:980	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	1	36	theme	complex	178:184	arg1	transitions					124:134	Soft tissue-to-bone transitions	104:134	Soft tissue-to-bone transitions	104:134	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	1	36	theme	complex	178:184	arg1	interface					163:171	the osteochondral interface	145:171	the osteochondral interface	145:171	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	1	36	theme	complex	178:184	arg1	junctions					186:194	complex junctions	178:194	complex junctions that connect multiple tissue types and are critical for musculoskeletal function	178:275	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	7	37	theme	relative	1182:1189	arg1	content					1200:1206	the relative collagen content	1178:1206	the relative collagen content	1178:1206	It was observed that the relative collagen content increased continuously from cartilage to bone, whereas proteoglycan peaked within the deep zone of cartilage.
24839262	5	38	theme	age	820:822	arg1	transition					792:801	the cartilage-to-bone transition	770:801	the cartilage-to-bone transition	770:801	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	5	38	theme	age	820:822	arg1	function					808:815	a function	806:815	a function of age	806:822	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	8	39	theme	alignment	1420:1428	arg1	degree					1401:1406	a higher degree	1392:1406	a higher degree of collagen alignment in both the surface and deep zone cartilage	1392:1472	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	10	40	theme	benchmark	1739:1747	arg1	parameters					1749:1758	critical benchmark parameters	1730:1758	critical benchmark parameters for current efforts in integrative cartilage repair	1730:1810	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
24839262	10	40	theme	benchmark	1739:1747	arg1	observations					1597:1608	These observations	1591:1608	These observations	1591:1608	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
24839262	7	41	theme	collagen	1191:1198	arg1	content					1200:1206	the relative collagen content	1178:1206	the relative collagen content	1178:1206	It was observed that the relative collagen content increased continuously from cartilage to bone, whereas proteoglycan peaked within the deep zone of cartilage.
24839262	1	42	theme	osteochondral	149:161	arg1	interface					163:171	the osteochondral interface	145:171	the osteochondral interface	145:171	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	8	43	from	surface	1442:1448	arg1	degree					1401:1406	a higher degree	1392:1406	a higher degree of collagen alignment in both the surface and deep zone cartilage	1392:1472	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	8	44	theme	deep	1454:1457	arg1	cartilage					1464:1472	deep zone cartilage	1454:1472	deep zone cartilage	1454:1472	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	0	45	theme	cartilage-to-bone	36:52	arg1	interface					54:62	the cartilage-to-bone interface	32:62	the cartilage-to-bone interface	32:62	FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age.
24839262	9	46	theme	mineral	1509:1515	arg1	content					1517:1523	mineral content	1509:1523	mineral content	1509:1523	Interestingly, regardless of age, mineral content increased exponentially across the calcified cartilage interface.
24839262	8	47	theme	zone	1459:1462	arg1	cartilage					1464:1472	deep zone cartilage	1454:1472	deep zone cartilage	1454:1472	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	4	48	with	interface	636:644	arg1	onset					655:659	the onset	651:659	the onset of skeletal maturity	651:680	Moreover, age-related changes at the interface with the onset of skeletal maturity are also not well understood.
24839262	10	49	theme	cartilage-to-bone	1685:1701	arg1	junction					1703:1710	the cartilage-to-bone junction	1681:1710	the cartilage-to-bone junction	1681:1710	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
24839262	5	50	theme	imaging	872:878	arg1	analysis					889:896	spectroscopic imaging (FTIR-I) analysis	858:896	spectroscopic imaging (FTIR-I) analysis	858:896	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	8	51	theme	collagen	1411:1418	arg1	alignment					1420:1428	collagen alignment	1411:1428	collagen alignment in both the surface and deep zone cartilage	1411:1472	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	1	52	theme	multiple	209:216	arg1	types					225:229	multiple tissue types	209:229	multiple tissue types	209:229	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	10	53	theme	cartilage	1795:1803	arg1	repair					1805:1810	integrative cartilage repair	1783:1810	integrative cartilage repair	1783:1810	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
24839262	8	54	theme	higher	1394:1399	arg1	degree					1401:1406	a higher degree	1392:1406	a higher degree of collagen alignment in both the surface and deep zone cartilage	1392:1472	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	2	55	theme	load	365:368	arg1	transfer					370:377	load transfer	365:377	load transfer between cartilage and bone	365:404	The osteochondral interface enables pressurization of articular cartilage, facilitates load transfer between cartilage and bone, and serves as a barrier between these two distinct tissues.
24839262	0	56	theme	interface	54:62	arg1	mapping					21:27	FTIR-I compositional mapping	0:27	FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age.	0:102	FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age.
24839262	9	57	theme	calcified	1560:1568	arg1	interface					1580:1588	the calcified cartilage interface	1556:1588	the calcified cartilage interface	1556:1588	Interestingly, regardless of age, mineral content increased exponentially across the calcified cartilage interface.
24839262	9	58	theme	cartilage	1570:1578	arg1	interface					1580:1588	the calcified cartilage interface	1556:1588	the calcified cartilage interface	1556:1588	Interestingly, regardless of age, mineral content increased exponentially across the calcified cartilage interface.
24839262	10	59	theme	current	1764:1770	arg1	efforts					1772:1778	current efforts	1764:1778	current efforts in integrative cartilage repair	1764:1810	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
24839262	7	60	theme	cartilage	1307:1315	arg1	zone					1299:1302	the deep zone	1290:1302	the deep zone of cartilage	1290:1315	It was observed that the relative collagen content increased continuously from cartilage to bone, whereas proteoglycan peaked within the deep zone of cartilage.
24839262	1	61	theme	tissue-to-bone	109:122	arg1	transitions					124:134	Soft tissue-to-bone transitions	104:134	Soft tissue-to-bone transitions	104:134	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	1	61	theme	tissue-to-bone	109:122	arg1	interface					163:171	the osteochondral interface	145:171	the osteochondral interface	145:171	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	1	61	theme	tissue-to-bone	109:122	arg1	junctions					186:194	complex junctions	178:194	complex junctions that connect multiple tissue types and are critical for musculoskeletal function	178:275	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	3	62	theme	multitissue	575:585	arg1	transition					587:596	this multitissue transition	570:596	this multitissue transition	570:596	Presently, there is a lack of quantitative understanding of the matrix and mineral distribution across this multitissue transition.
24839262	8	63	theme	collagen	1328:1335	arg1	content					1337:1343	collagen content	1328:1343	collagen content across the interface	1328:1364	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	1	64	theme	tissue	218:223	arg1	types					225:229	multiple tissue types	209:229	multiple tissue types	209:229	Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function.
24839262	5	65	theme	collagen	994:1001	arg1	organization					1003:1014	collagen organization	994:1014	collagen organization	994:1014	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	2	66	theme	cartilage	342:350	arg1	pressurization					314:327	pressurization	314:327	pressurization of articular cartilage	314:350	The osteochondral interface enables pressurization of articular cartilage, facilitates load transfer between cartilage and bone, and serves as a barrier between these two distinct tissues.
24839262	8	67	from	alignment	1420:1428	arg1	surface					1442:1448	the surface	1438:1448	the surface	1438:1448	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	8	67	from	alignment	1420:1428	arg1	cartilage					1464:1472	deep zone cartilage	1454:1472	deep zone cartilage	1454:1472	With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage.
24839262	4	68	from	interface	636:644	arg1	changes					621:627	age-related changes	609:627	age-related changes at the interface with the onset of skeletal maturity	609:680	Moreover, age-related changes at the interface with the onset of skeletal maturity are also not well understood.
24839262	5	69	theme	spectroscopic	858:870	arg1	analysis					889:896	spectroscopic imaging (FTIR-I) analysis	858:896	spectroscopic imaging (FTIR-I) analysis	858:896	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	5	70	dep	Fourier	831:837	arg1	transform					839:847	transform	839:847	transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization	839:1014	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	10	71	theme	integrative	1783:1793	arg1	repair					1805:1810	integrative cartilage repair	1783:1810	integrative cartilage repair	1783:1810	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
24839262	6	72	theme	tissue-dependent	1022:1037	arg1	changes					1055:1061	Both tissue-dependent and age-related changes	1017:1061	Both tissue-dependent and age-related changes	1017:1061	Both tissue-dependent and age-related changes were observed, underscoring the role of postnatal physiological loading in matrix remodeling.
24839262	4	73	theme	age-related	609:619	arg1	changes					621:627	age-related changes	609:627	age-related changes at the interface with the onset of skeletal maturity	609:680	Moreover, age-related changes at the interface with the onset of skeletal maturity are also not well understood.
24839262	5	74	dep	transform	839:847	arg1	infrared					849:856	infrared	849:856	transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization	839:1014	Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization.
24839262	6	75	theme	loading	1127:1133	arg1	role					1095:1098	the role	1091:1098	the role of postnatal physiological loading in matrix remodeling	1091:1154	Both tissue-dependent and age-related changes were observed, underscoring the role of postnatal physiological loading in matrix remodeling.
24839262	10	76	theme	new	1617:1619	arg1	insights					1621:1628	new insights	1617:1628	new insights into both region- and age-dependent changes across the cartilage-to-bone junction	1617:1710	These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.
25694539	4	0	theme	volume	867:872	arg1	fraction					874:881	a high mineral volume fraction	852:881	a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size	852:971	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	1	1	theme	goethite	160:167	arg1	nanofibres					169:178	reinforcing goethite nanofibres	148:178	reinforcing goethite nanofibres	148:178	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25694539	1	2	theme	rock	260:263	arg1	surfaces					265:272	rock surfaces	260:272	rock surfaces	260:272	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25694539	2	3	theme	size	472:475	arg1	independent					450:460	independent	450:460	independent	450:460	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	2	3	theme	size	472:475	arg1	strength					302:309	The tensile strength	290:309	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy	290:401	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	3	4	theme	strongest	679:687	arg1	material					697:704	the strongest natural material	675:704	the strongest natural material	675:704	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	4	5	theme	mineral	859:865	arg1	fraction					874:881	a high mineral volume fraction	852:881	a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size	852:971	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	1	6	theme	nanofibres	169:178	arg1	fractions					135:143	high volume fractions	123:143	high volume fractions of reinforcing goethite nanofibres	123:178	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25694539	2	7	from	GPa	438:440	arg1	range					416:420	range	416:420	range from 3.0 to 6.5 GPa	416:440	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	2	8	dep	in	371:372	arg1	situ					374:377	situ	374:377	situ	374:377	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	4	9	theme	critical	959:966	arg1	size					968:971	a defect-controlled critical size	939:971	a defect-controlled critical size	939:971	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	3	10	dep	highest	557:563	arg1	approaching					711:721	approaching	711:721	approaching values comparable to those of the strongest man-made fibres	711:781	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	3	10	dep	highest	557:563	arg1	outperforming					601:613	outperforming	601:613	outperforming the high strength of spider silk currently considered to be the strongest natural material	601:704	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	2	11	theme	tensile	294:300	arg1	independent					450:460	independent	450:460	independent	450:460	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	2	11	theme	tensile	294:300	arg1	strength					302:309	The tensile strength	290:309	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy	290:401	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	3	12	theme	comparable	730:739	arg1	values					723:728	values	723:728	values comparable to those of the strongest man-made fibres	723:781	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	2	13	dep	6.5	434:436	arg1	to					431:432	to	431:432	to	431:432	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	1	14	theme	limpets	56:62	arg1	teeth					47:51	The teeth	43:51	The teeth of limpets	43:62	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25694539	4	15	from	design	998:1003	arg1	teeth					1015:1019	limpet teeth	1008:1019	limpet teeth	1008:1019	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	4	16	theme	theoretical	1042:1052	arg1	limits					1063:1068	theoretical strength limits	1042:1068	theoretical strength limits	1042:1068	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	3	17	theme	material	519:526	arg1	highest					557:563	highest	557:563	highest	557:563	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	3	17	theme	material	519:526	arg1	strength					536:543	an absolute material tensile strength	507:543	an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres	507:781	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	4	18	theme	high	854:857	arg1	fraction					874:881	a high mineral volume fraction	852:881	a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size	852:971	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	1	19	theme	distinctive	72:82	arg1	nanostructures					94:107	distinctive composite nanostructures	72:107	distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres	72:178	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25694539	1	20	theme	softer	189:194	arg1	phase					204:208	a softer protein phase	187:208	a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding	187:287	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25694539	0	21	theme	Extreme	0:6	arg1	strength					8:15	Extreme strength	0:15	Extreme strength	0:15	Extreme strength observed in limpet teeth.
25694539	1	22	theme	composite	84:92	arg1	nanostructures					94:107	distinctive composite nanostructures	72:107	distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres	72:178	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25694539	2	23	theme	force	386:390	arg1	microscopy					392:401	in situ atomic force microscopy	371:401	in situ atomic force microscopy	371:401	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	4	24	theme	teeth	829:833	arg1	strength					810:817	This considerable tensile strength	784:817	This considerable tensile strength of limpet teeth	784:833	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	2	25	theme	discrete	314:321	arg1	volumes					323:329	discrete volumes	314:329	discrete volumes of limpet tooth material measured using in situ atomic force microscopy	314:401	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	4	26	theme	limpet	822:827	arg1	teeth					829:833	limpet teeth	822:833	limpet teeth	822:833	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	2	27	theme	volumes	323:329	arg1	independent					450:460	independent	450:460	independent	450:460	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	2	27	theme	volumes	323:329	arg1	strength					302:309	The tensile strength	290:309	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy	290:401	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	3	28	theme	tensile	528:534	arg1	highest					557:563	highest	557:563	highest	557:563	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	3	28	theme	tensile	528:534	arg1	strength					536:543	an absolute material tensile strength	507:543	an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres	507:781	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	3	29	theme	silk	643:646	arg1	strength					624:631	the high strength	615:631	the high strength of spider silk currently considered to be the strongest natural material	615:704	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	3	30	theme	absolute	510:517	arg1	highest					557:563	highest	557:563	highest	557:563	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	3	30	theme	absolute	510:517	arg1	strength					536:543	an absolute material tensile strength	507:543	an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres	507:781	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	2	31	theme	sample	465:470	arg1	size					472:475	sample size	465:475	sample size	465:475	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	2	32	theme	material	347:354	arg1	volumes					323:329	discrete volumes	314:329	discrete volumes of limpet tooth material measured using in situ atomic force microscopy	314:401	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	4	33	theme	defect-controlled	941:957	arg1	size					968:971	a defect-controlled critical size	939:971	a defect-controlled critical size	939:971	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	4	34	with	nanofibres	907:916	arg1	diameters					923:931	diameters	923:931	diameters below a defect-controlled critical size	923:971	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	4	35	theme	tensile	802:808	arg1	strength					810:817	This considerable tensile strength	784:817	This considerable tensile strength of limpet teeth	784:833	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	1	36	theme	protein	196:202	arg1	phase					204:208	a softer protein phase	187:208	a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding	187:287	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25694539	0	37	theme	limpet	29:34	arg1	teeth					36:40	limpet teeth	29:40	limpet teeth	29:40	Extreme strength observed in limpet teeth.
25694539	2	38	theme	in	371:372	arg1	microscopy					392:401	in situ atomic force microscopy	371:401	in situ atomic force microscopy	371:401	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	4	39	theme	limpet	1008:1013	arg1	teeth					1015:1019	limpet teeth	1008:1019	limpet teeth	1008:1019	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	1	40	theme	mechanical	221:230	arg1	integrity					232:240	mechanical integrity	221:240	mechanical integrity	221:240	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25694539	4	41	theme	goethite	898:905	arg1	nanofibres					907:916	reinforcing goethite nanofibres	886:916	reinforcing goethite nanofibres with diameters below a defect-controlled critical size	886:971	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	3	42	theme	spider	636:641	arg1	silk					643:646	spider silk	636:646	spider silk currently considered to be the strongest natural material	636:704	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	3	43	theme	natural	689:695	arg1	material					697:704	the strongest natural material	675:704	the strongest natural material	675:704	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	3	44	theme	strongest	757:765	arg1	fibres					776:781	the strongest man-made fibres	753:781	the strongest man-made fibres	753:781	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	4	45	theme	reinforcing	886:896	arg1	nanofibres					907:916	reinforcing goethite nanofibres	886:916	reinforcing goethite nanofibres with diameters below a defect-controlled critical size	886:971	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	3	46	theme	biological	580:589	arg1	material					591:598	a biological material	578:598	a biological material	578:598	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	1	47	theme	high	123:126	arg1	fractions					135:143	high volume fractions	123:143	high volume fractions of reinforcing goethite nanofibres	123:178	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25694539	3	48	theme	man-made	767:774	arg1	fibres					776:781	the strongest man-made fibres	753:781	the strongest man-made fibres	753:781	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	4	49	theme	considerable	789:800	arg1	strength					810:817	This considerable tensile strength	784:817	This considerable tensile strength of limpet teeth	784:833	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	3	50	theme	high	619:622	arg1	strength					624:631	the high strength	615:631	the high strength of spider silk currently considered to be the strongest natural material	615:704	These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres.
25694539	1	51	theme	volume	128:133	arg1	fractions					135:143	high volume fractions	123:143	high volume fractions of reinforcing goethite nanofibres	123:178	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25694539	4	52	theme	nanofibres	907:916	arg1	fraction					874:881	a high mineral volume fraction	852:881	a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size	852:971	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	2	53	theme	limpet	334:339	arg1	material					347:354	limpet tooth material	334:354	limpet tooth material	334:354	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	2	54	theme	tooth	341:345	arg1	material					347:354	limpet tooth material	334:354	limpet tooth material	334:354	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	4	55	theme	natural	990:996	arg1	design					998:1003	natural design	990:1003	natural design in limpet teeth	990:1019	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	2	56	theme	atomic	379:384	arg1	microscopy					392:401	in situ atomic force microscopy	371:401	in situ atomic force microscopy	371:401	The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size.
25694539	4	57	theme	strength	1054:1061	arg1	limits					1063:1068	theoretical strength limits	1042:1068	theoretical strength limits	1042:1068	This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.
25694539	1	58	theme	reinforcing	148:158	arg1	nanofibres					169:178	reinforcing goethite nanofibres	148:178	reinforcing goethite nanofibres	148:178	The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding.
25790730	6	0	theme	tagged	933:938	arg1	nanoparticles					940:952	These fluorescently tagged nanoparticles	913:952	These fluorescently tagged nanoparticles	913:952	These fluorescently tagged nanoparticles were both filtered and internalized by renal tubular epithelial cells in a dose- and time-dependent fashion.
25790730	11	1	theme	renal	1635:1639	arg1	effects					1641:1647	no major detrimental renal effects	1614:1647	no major detrimental renal effects of DNP on overall kidney function	1614:1681	We conclude that there are no major detrimental renal effects of DNP on overall kidney function, but changes in endocytosis-mediating protein expression do occur.
25790730	5	2	theme	dendrimer	863:871	arg1	nanoparticles					873:885	poly(amido amine) dendrimer nanoparticles	845:885	poly(amido amine) dendrimer nanoparticles	845:885	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	8	3	from	increase	1240:1247	arg1	output					1260:1265	urinary output	1252:1265	urinary output	1252:1265	DNP administration resulted in a dose-dependent increase in urinary output, while cellular albumin endocytosis was increased.
25790730	9	4	theme	AQP1	1408:1411	arg1	expression					1413:1422	AQP1 expression	1408:1422	AQP1 expression	1408:1422	The expression of megalin, a receptor involved in albumin uptake, was also increased, but AQP1 expression was unaffected.
25790730	5	5	theme	dextran-based	750:762	arg1	DNP					779:781	DNP	779:781	DNP	779:781	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	5	5	theme	dextran-based	750:762	arg1	nanoparticles					764:776	either 5 nm dextran-based nanoparticles	738:776	either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size	738:910	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	11	6	theme	DNP	1652:1654	arg1	effects					1641:1647	no major detrimental renal effects	1614:1647	no major detrimental renal effects of DNP on overall kidney function	1614:1681	We conclude that there are no major detrimental renal effects of DNP on overall kidney function, but changes in endocytosis-mediating protein expression do occur.
25790730	1	7	theme	novel	166:170	arg1	diagnostics					172:182	novel diagnostics	166:182	novel diagnostics	166:182	Nanotechnology approaches are actively being pursued for drug delivery, novel diagnostics, implantable devices, and consumer products.
25790730	12	8	theme	preparations	1827:1838	arg1	testing					1792:1798	the testing	1788:1798	the testing of additional nanoparticle preparations as they become available	1788:1863	These studies provide a framework for the testing of additional nanoparticle preparations as they become available.
25790730	5	9	from	similar	793:799	arg1	composition					804:814	composition	804:814	composition	804:814	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	11	10	theme	major	1617:1621	arg1	effects					1641:1647	no major detrimental renal effects	1614:1647	no major detrimental renal effects of DNP on overall kidney function	1614:1681	We conclude that there are no major detrimental renal effects of DNP on overall kidney function, but changes in endocytosis-mediating protein expression do occur.
25790730	4	11	theme	filtered	584:591	arg1	case					625:628	the case	621:628	the case for filtered nanoparticles	621:655	The active endocytic machinery of kidney proximal tubules avidly internalizes filtered proteins, which may also be the case for filtered nanoparticles.
25790730	4	11	theme	filtered	584:591	arg1	proteins					593:600	filtered proteins	584:600	filtered proteins	584:600	The active endocytic machinery of kidney proximal tubules avidly internalizes filtered proteins, which may also be the case for filtered nanoparticles.
25790730	11	12	theme	kidney	1667:1672	arg1	function					1674:1681	overall kidney function	1659:1681	overall kidney function	1659:1681	We conclude that there are no major detrimental renal effects of DNP on overall kidney function, but changes in endocytosis-mediating protein expression do occur.
25790730	5	13	theme	size	907:910	arg1	nanoparticles					873:885	poly(amido amine) dendrimer nanoparticles	845:885	poly(amido amine) dendrimer nanoparticles	845:885	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	5	13	theme	size	907:910	arg1	materials					832:840	FDA-approved materials	819:840	FDA-approved materials	819:840	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	5	13	theme	size	907:910	arg1	PNP					888:890	PNP	888:890	PNP	888:890	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	5	14	theme	poly	845:848	arg1	nanoparticles					873:885	poly(amido amine) dendrimer nanoparticles	845:885	poly(amido amine) dendrimer nanoparticles	845:885	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	6	15	theme	time-dependent	1039:1052	arg1	fashion					1054:1060	a dose- and time-dependent fashion	1027:1060	a dose- and time-dependent fashion	1027:1060	These fluorescently tagged nanoparticles were both filtered and internalized by renal tubular epithelial cells in a dose- and time-dependent fashion.
25790730	8	16	theme	urinary	1252:1258	arg1	output					1260:1265	urinary output	1252:1265	urinary output	1252:1265	DNP administration resulted in a dose-dependent increase in urinary output, while cellular albumin endocytosis was increased.
25790730	8	17	theme	dose-dependent	1225:1238	arg1	increase					1240:1247	a dose-dependent increase	1223:1247	a dose-dependent increase in urinary output	1223:1265	DNP administration resulted in a dose-dependent increase in urinary output, while cellular albumin endocytosis was increased.
25790730	9	18	theme	megalin	1336:1342	arg1	expression					1322:1331	The expression	1318:1331	The expression of megalin, a receptor involved in albumin uptake,	1318:1382	The expression of megalin, a receptor involved in albumin uptake, was also increased, but AQP1 expression was unaffected.
25790730	7	19	theme	injury	1144:1149	arg1	markers					1151:1157	kidney injury markers	1137:1157	kidney injury markers	1137:1157	The biological effects were quantitated by immunocytochemistry, measuring kidney injury markers and performing functional tests.
25790730	3	20	theme	supersmall	438:447	arg1	nanoparticles					449:461	supersmall nanoparticles	438:461	supersmall nanoparticles (<10 nm)	438:470	This becomes critical for supersmall nanoparticles (<10 nm), designed to be renally excreted.
25790730	3	20	theme	supersmall	438:447	arg1	nm					468:469	<10 nm	464:469	<10 nm	464:469	This becomes critical for supersmall nanoparticles (<10 nm), designed to be renally excreted.
25790730	7	21	theme	functional	1174:1183	arg1	tests					1185:1189	functional tests	1174:1189	functional tests	1174:1189	The biological effects were quantitated by immunocytochemistry, measuring kidney injury markers and performing functional tests.
25790730	10	22	theme	increased	1553:1561	arg1	endocytosis					1563:1573	increased endocytosis	1553:1573	increased endocytosis of dextran	1553:1584	The effects after PNP administration were similar but additionally resulted in increased clathrin expression and increased endocytosis of dextran.
25790730	5	23	dep	nanoparticles	764:776	arg1	nm					747:748	nm	747:748	nm	747:748	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	5	24	theme	comparable	896:905	arg1	size					907:910	comparable size	896:910	comparable size	896:910	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	11	25	from	effects	1641:1647	arg1	function					1674:1681	overall kidney function	1659:1681	overall kidney function	1659:1681	We conclude that there are no major detrimental renal effects of DNP on overall kidney function, but changes in endocytosis-mediating protein expression do occur.
25790730	1	26	theme	implantable	185:195	arg1	devices					197:203	implantable devices	185:203	implantable devices	185:203	Nanotechnology approaches are actively being pursued for drug delivery, novel diagnostics, implantable devices, and consumer products.
25790730	4	27	theme	kidney	540:545	arg1	tubules					556:562	kidney proximal tubules	540:562	kidney proximal tubules	540:562	The active endocytic machinery of kidney proximal tubules avidly internalizes filtered proteins, which may also be the case for filtered nanoparticles.
25790730	7	28	theme	biological	1067:1076	arg1	effects					1078:1084	The biological effects	1063:1084	The biological effects	1063:1084	The biological effects were quantitated by immunocytochemistry, measuring kidney injury markers and performing functional tests.
25790730	1	29	theme	Nanotechnology	94:107	arg1	approaches					109:118	Nanotechnology approaches	94:118	Nanotechnology approaches	94:118	Nanotechnology approaches are actively being pursued for drug delivery, novel diagnostics, implantable devices, and consumer products.
25790730	5	30	theme	amido	850:854	arg1	nanoparticles					873:885	poly(amido amine) dendrimer nanoparticles	845:885	poly(amido amine) dendrimer nanoparticles	845:885	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	5	31	dep	nm	747:748	arg1	5					745:745	5	745:745	5	745:745	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	10	32	theme	PNP	1458:1460	arg1	administration					1462:1475	PNP administration	1458:1475	PNP administration	1458:1475	The effects after PNP administration were similar but additionally resulted in increased clathrin expression and increased endocytosis of dextran.
25790730	6	33	theme	dose-	1029:1033	arg1	fashion					1054:1060	a dose- and time-dependent fashion	1027:1060	a dose- and time-dependent fashion	1027:1060	These fluorescently tagged nanoparticles were both filtered and internalized by renal tubular epithelial cells in a dose- and time-dependent fashion.
25790730	2	34	theme	targeted	313:320	arg1	tumor					322:326	targeted tumor or phagocytic cells	313:346	tumor	322:326	While considerable research has been performed on the effects of these materials on targeted tumor or phagocytic cells, relatively little is known about their effects on renal cells.
25790730	4	35	theme	tubules	556:562	arg1	machinery					527:535	The active endocytic machinery	506:535	The active endocytic machinery of kidney proximal tubules	506:562	The active endocytic machinery of kidney proximal tubules avidly internalizes filtered proteins, which may also be the case for filtered nanoparticles.
25790730	5	36	theme	kidney	699:704	arg1	function					706:713	kidney function	699:713	kidney function	699:713	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	11	37	theme	protein	1721:1727	arg1	expression					1729:1738	endocytosis-mediating protein expression	1699:1738	endocytosis-mediating protein expression	1699:1738	We conclude that there are no major detrimental renal effects of DNP on overall kidney function, but changes in endocytosis-mediating protein expression do occur.
25790730	2	38	theme	materials	300:308	arg1	effects					283:289	the effects	279:289	the effects of these materials on targeted tumor or phagocytic cells	279:346	While considerable research has been performed on the effects of these materials on targeted tumor or phagocytic cells, relatively little is known about their effects on renal cells.
25790730	5	39	theme	amine	856:860	arg1	nanoparticles					873:885	poly(amido amine) dendrimer nanoparticles	845:885	poly(amido amine) dendrimer nanoparticles	845:885	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	0	40	theme	nanoparticles	43:55	arg1	interactions					19:30	the interactions	15:30	the interactions of organic nanoparticles with renal epithelial cells	15:83	Characterizing the interactions of organic nanoparticles with renal epithelial cells in vivo.
25790730	4	41	theme	proximal	547:554	arg1	tubules					556:562	kidney proximal tubules	540:562	kidney proximal tubules	540:562	The active endocytic machinery of kidney proximal tubules avidly internalizes filtered proteins, which may also be the case for filtered nanoparticles.
25790730	5	42	theme	FDA-approved	819:830	arg1	PNP					888:890	PNP	888:890	PNP	888:890	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	5	42	theme	FDA-approved	819:830	arg1	materials					832:840	FDA-approved materials	819:840	FDA-approved materials	819:840	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	1	43	theme	consumer	210:217	arg1	products					219:226	consumer products	210:226	consumer products	210:226	Nanotechnology approaches are actively being pursued for drug delivery, novel diagnostics, implantable devices, and consumer products.
25790730	0	44	theme	organic	35:41	arg1	nanoparticles					43:55	organic nanoparticles	35:55	organic nanoparticles	35:55	Characterizing the interactions of organic nanoparticles with renal epithelial cells in vivo.
25790730	4	45	theme	active	510:515	arg1	machinery					527:535	The active endocytic machinery	506:535	The active endocytic machinery of kidney proximal tubules	506:562	The active endocytic machinery of kidney proximal tubules avidly internalizes filtered proteins, which may also be the case for filtered nanoparticles.
25790730	2	46	theme	considerable	235:246	arg1	research					248:255	considerable research	235:255	considerable research	235:255	While considerable research has been performed on the effects of these materials on targeted tumor or phagocytic cells, relatively little is known about their effects on renal cells.
25790730	2	47	theme	renal	399:403	arg1	cells					405:409	renal cells	399:409	renal cells	399:409	While considerable research has been performed on the effects of these materials on targeted tumor or phagocytic cells, relatively little is known about their effects on renal cells.
25790730	0	48	theme	renal	62:66	arg1	cells					79:83	renal epithelial cells	62:83	renal epithelial cells	62:83	Characterizing the interactions of organic nanoparticles with renal epithelial cells in vivo.
25790730	9	49	theme	albumin	1368:1374	arg1	uptake					1376:1381	albumin uptake	1368:1381	albumin uptake	1368:1381	The expression of megalin, a receptor involved in albumin uptake, was also increased, but AQP1 expression was unaffected.
25790730	11	50	theme	endocytosis-mediating	1699:1719	arg1	expression					1729:1738	endocytosis-mediating protein expression	1699:1738	endocytosis-mediating protein expression	1699:1738	We conclude that there are no major detrimental renal effects of DNP on overall kidney function, but changes in endocytosis-mediating protein expression do occur.
25790730	0	51	with	interactions	19:30	arg1	cells					79:83	renal epithelial cells	62:83	renal epithelial cells	62:83	Characterizing the interactions of organic nanoparticles with renal epithelial cells in vivo.
25790730	7	52	theme	kidney	1137:1142	arg1	markers					1151:1157	kidney injury markers	1137:1157	kidney injury markers	1137:1157	The biological effects were quantitated by immunocytochemistry, measuring kidney injury markers and performing functional tests.
25790730	5	53	from	composition	804:814	arg1	similar					793:799	similar	793:799	similar	793:799	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	2	54	from	effects	283:289	arg1	cells					342:346	targeted tumor or phagocytic cells	313:346	cells	342:346	While considerable research has been performed on the effects of these materials on targeted tumor or phagocytic cells, relatively little is known about their effects on renal cells.
25790730	2	54	from	effects	283:289	arg1	tumor					322:326	targeted tumor or phagocytic cells	313:346	tumor	322:326	While considerable research has been performed on the effects of these materials on targeted tumor or phagocytic cells, relatively little is known about their effects on renal cells.
25790730	4	55	theme	endocytic	517:525	arg1	machinery					527:535	The active endocytic machinery	506:535	The active endocytic machinery of kidney proximal tubules	506:562	The active endocytic machinery of kidney proximal tubules avidly internalizes filtered proteins, which may also be the case for filtered nanoparticles.
25790730	5	56	theme	such	674:677	arg1	interactions					679:690	such interactions	674:690	such interactions	674:690	To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size.
25790730	2	57	from	effects	388:394	arg1	cells					405:409	renal cells	399:409	renal cells	399:409	While considerable research has been performed on the effects of these materials on targeted tumor or phagocytic cells, relatively little is known about their effects on renal cells.
25790730	2	58	theme	phagocytic	331:340	arg1	cells					342:346	targeted tumor or phagocytic cells	313:346	cells	342:346	While considerable research has been performed on the effects of these materials on targeted tumor or phagocytic cells, relatively little is known about their effects on renal cells.
25790730	6	59	theme	epithelial	1007:1016	arg1	cells					1018:1022	renal tubular epithelial cells	993:1022	renal tubular epithelial cells	993:1022	These fluorescently tagged nanoparticles were both filtered and internalized by renal tubular epithelial cells in a dose- and time-dependent fashion.
25790730	10	60	theme	increased	1519:1527	arg1	expression					1538:1547	increased clathrin expression	1519:1547	increased clathrin expression	1519:1547	The effects after PNP administration were similar but additionally resulted in increased clathrin expression and increased endocytosis of dextran.
25790730	12	61	theme	nanoparticle	1814:1825	arg1	preparations					1827:1838	additional nanoparticle preparations	1803:1838	additional nanoparticle preparations as they become available	1803:1863	These studies provide a framework for the testing of additional nanoparticle preparations as they become available.
25790730	10	62	theme	clathrin	1529:1536	arg1	expression					1538:1547	increased clathrin expression	1519:1547	increased clathrin expression	1519:1547	The effects after PNP administration were similar but additionally resulted in increased clathrin expression and increased endocytosis of dextran.
25790730	12	63	theme	additional	1803:1812	arg1	preparations					1827:1838	additional nanoparticle preparations	1803:1838	additional nanoparticle preparations as they become available	1803:1863	These studies provide a framework for the testing of additional nanoparticle preparations as they become available.
25790730	6	64	theme	tubular	999:1005	arg1	cells					1018:1022	renal tubular epithelial cells	993:1022	renal tubular epithelial cells	993:1022	These fluorescently tagged nanoparticles were both filtered and internalized by renal tubular epithelial cells in a dose- and time-dependent fashion.
25790730	10	65	theme	dextran	1578:1584	arg1	expression					1538:1547	increased clathrin expression	1519:1547	increased clathrin expression	1519:1547	The effects after PNP administration were similar but additionally resulted in increased clathrin expression and increased endocytosis of dextran.
25790730	10	65	theme	dextran	1578:1584	arg1	endocytosis					1563:1573	increased endocytosis	1553:1573	increased endocytosis of dextran	1553:1584	The effects after PNP administration were similar but additionally resulted in increased clathrin expression and increased endocytosis of dextran.
25790730	8	66	theme	albumin	1283:1289	arg1	endocytosis					1291:1301	cellular albumin endocytosis	1274:1301	cellular albumin endocytosis	1274:1301	DNP administration resulted in a dose-dependent increase in urinary output, while cellular albumin endocytosis was increased.
25790730	8	67	theme	cellular	1274:1281	arg1	endocytosis					1291:1301	cellular albumin endocytosis	1274:1301	cellular albumin endocytosis	1274:1301	DNP administration resulted in a dose-dependent increase in urinary output, while cellular albumin endocytosis was increased.
25790730	1	68	theme	drug	151:154	arg1	delivery					156:163	drug delivery	151:163	drug delivery	151:163	Nanotechnology approaches are actively being pursued for drug delivery, novel diagnostics, implantable devices, and consumer products.
25790730	4	69	theme	filtered	634:641	arg1	nanoparticles					643:655	filtered nanoparticles	634:655	filtered nanoparticles	634:655	The active endocytic machinery of kidney proximal tubules avidly internalizes filtered proteins, which may also be the case for filtered nanoparticles.
25790730	6	70	theme	renal	993:997	arg1	cells					1018:1022	renal tubular epithelial cells	993:1022	renal tubular epithelial cells	993:1022	These fluorescently tagged nanoparticles were both filtered and internalized by renal tubular epithelial cells in a dose- and time-dependent fashion.
25790730	0	71	theme	epithelial	68:77	arg1	cells					79:83	renal epithelial cells	62:83	renal epithelial cells	62:83	Characterizing the interactions of organic nanoparticles with renal epithelial cells in vivo.
25790730	11	72	theme	overall	1659:1665	arg1	function					1674:1681	overall kidney function	1659:1681	overall kidney function	1659:1681	We conclude that there are no major detrimental renal effects of DNP on overall kidney function, but changes in endocytosis-mediating protein expression do occur.
25790730	11	73	from	changes	1688:1694	arg1	expression					1729:1738	endocytosis-mediating protein expression	1699:1738	endocytosis-mediating protein expression	1699:1738	We conclude that there are no major detrimental renal effects of DNP on overall kidney function, but changes in endocytosis-mediating protein expression do occur.
25790730	11	74	theme	detrimental	1623:1633	arg1	effects					1641:1647	no major detrimental renal effects	1614:1647	no major detrimental renal effects of DNP on overall kidney function	1614:1681	We conclude that there are no major detrimental renal effects of DNP on overall kidney function, but changes in endocytosis-mediating protein expression do occur.
25790730	8	75	theme	DNP	1192:1194	arg1	administration					1196:1209	DNP administration	1192:1209	DNP administration	1192:1209	DNP administration resulted in a dose-dependent increase in urinary output, while cellular albumin endocytosis was increased.
27387003	8	0	theme	rate	1178:1181	arg1	inhibition					1145:1154	no inhibition	1142:1154	no inhibition of the average growth rate and biomass production	1142:1204	CE(50) for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC(50) for P. reticulata was 23% (v v(-1)).
27387003	3	1	theme	Al	531:532	arg1	sulphate					521:528	aluminium sulphate	511:528	aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O)	511:556	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	3	1	theme	Al	531:532	arg1	O					555:555	Al(2) (SO(4))(3).14 H(2)O	531:555	Al(2) (SO(4))(3).14 H(2)O	531:555	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	10	2	from	alternative	1403:1413	arg1	post-treatment					1422:1435	the post-treatment	1418:1435	the post-treatment of landfill leachate	1418:1456	Therefore, chitosan as primary coagulant was not considered to be a viable alternative in the post-treatment of landfill leachate.
27387003	1	3	theme	organic	288:294	arg1	matter					296:301	removing recalcitrant organic matter	266:301	removing recalcitrant organic matter	266:301	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	0	4	theme	primary	91:97	arg1	coagulant					99:107	primary coagulant	91:107	primary coagulant	91:107	Post-treatment of sanitary landfill leachate by coagulation-flocculation using chitosan as primary coagulant.
27387003	6	5	theme	efficiency	833:842	arg1	values					803:808	Mean values	798:808	Mean values of true colour removal efficiency of 80%	798:849	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	6	5	theme	efficiency	833:842	arg1	efficiency					873:882	turbidity removal efficiency	855:882	turbidity removal efficiency of 91.4%	855:891	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	1	6	theme	complementary	171:183	arg1	treatment					210:218	a complementary coagulation-flocculation treatment	169:218	a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter	169:301	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	8	7	theme	minor	1099:1103	arg1	L.					1096:1097	L. minor	1096:1103	L. minor	1096:1103	CE(50) for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC(50) for P. reticulata was 23% (v v(-1)).
27387003	1	8	theme	matter	296:301	arg1	aim					259:261	the aim	255:261	the aim of removing recalcitrant organic matter	255:301	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	6	9	theme	removal	825:831	arg1	efficiency					833:842	true colour removal efficiency	813:842	true colour removal efficiency of 80%	813:849	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	8	10	theme	production	1195:1204	arg1	inhibition					1145:1154	no inhibition	1142:1154	no inhibition of the average growth rate and biomass production	1142:1204	CE(50) for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC(50) for P. reticulata was 23% (v v(-1)).
27387003	2	11	theme	process	329:335	arg1	conditions					337:346	the process conditions	325:346	the process conditions	325:346	In order to optimize the process conditions, central composite design and response surface methodology were applied.
27387003	3	12	dep	Al	531:532	arg1	4					541:541	4	541:541	4	541:541	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	3	12	dep	Al	531:532	arg1	SO					538:539	SO	538:539	SO	538:539	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	6	13	theme	colour	818:823	arg1	efficiency					833:842	true colour removal efficiency	813:842	true colour removal efficiency of 80%	813:849	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	8	14	theme	biomass	1187:1193	arg1	production					1195:1204	biomass production	1187:1204	biomass production	1187:1204	CE(50) for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC(50) for P. reticulata was 23% (v v(-1)).
27387003	1	15	from	coagulant	156:164	arg1	treatment					210:218	a complementary coagulation-flocculation treatment	169:218	a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter	169:301	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	4	16	theme	Poecilia	669:676	arg1	reticulata					678:687	the guppy fish Poecilia reticulata	654:687	the guppy fish Poecilia reticulata	654:687	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	16	theme	Poecilia	669:676	arg1	organisms					697:705	test organisms	692:705	test organisms	692:705	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	16	theme	Poecilia	669:676	arg1	Lemna					638:642	the duckweed Lemna	625:642	the duckweed Lemna minor	625:648	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	1	17	theme	primary	148:154	arg1	Chitosan					110:117	Chitosan	110:117	Chitosan	110:117	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	1	17	theme	primary	148:154	arg1	coagulant					156:164	an alternative primary coagulant	133:164	an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter	133:301	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	4	18	theme	duckweed	629:636	arg1	reticulata					678:687	the guppy fish Poecilia reticulata	654:687	the guppy fish Poecilia reticulata	654:687	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	18	theme	duckweed	629:636	arg1	organisms					697:705	test organisms	692:705	test organisms	692:705	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	18	theme	duckweed	629:636	arg1	Lemna					638:642	the duckweed Lemna	625:642	the duckweed Lemna minor	625:648	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	7	19	theme	toxicity	963:970	arg1	tests					972:976	The acute toxicity tests	953:976	The acute toxicity tests	953:976	The acute toxicity tests showed that organisms were sensitive to all samples, mainly after coagulation-flocculation using chitosan.
27387003	9	20	theme	Multi-criteria	1251:1264	arg1	analysis					1266:1273	Multi-criteria analysis	1251:1273	Multi-criteria analysis	1251:1273	Multi-criteria analysis showed that alum was the most appropriate coagulant.
27387003	4	21	theme	test	692:695	arg1	reticulata					678:687	the guppy fish Poecilia reticulata	654:687	the guppy fish Poecilia reticulata	654:687	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	21	theme	test	692:695	arg1	organisms					697:705	test organisms	692:705	test organisms	692:705	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	21	theme	test	692:695	arg1	Lemna					638:642	the duckweed Lemna	625:642	the duckweed Lemna minor	625:648	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	1	22	theme	coagulation-flocculation	185:208	arg1	treatment					210:218	a complementary coagulation-flocculation treatment	169:218	a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter	169:301	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	5	23	theme	analytic	712:719	arg1	process					731:737	The analytic hierarchy process	708:737	The analytic hierarchy process	708:737	The analytic hierarchy process was employed for selecting the most appropriate coagulant.
27387003	6	24	theme	chitosan	909:916	arg1	dosages					918:924	chitosan dosages	909:924	chitosan dosages of 960 mg L(-1)	909:940	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	10	25	theme	landfill	1440:1447	arg1	leachate					1449:1456	landfill leachate	1440:1456	landfill leachate	1440:1456	Therefore, chitosan as primary coagulant was not considered to be a viable alternative in the post-treatment of landfill leachate.
27387003	5	26	theme	hierarchy	721:729	arg1	process					731:737	The analytic hierarchy process	708:737	The analytic hierarchy process	708:737	The analytic hierarchy process was employed for selecting the most appropriate coagulant.
27387003	2	27	theme	central	349:355	arg1	design					367:372	central composite design	349:372	central composite design	349:372	In order to optimize the process conditions, central composite design and response surface methodology were applied.
27387003	6	28	theme	true	813:816	arg1	efficiency					833:842	true colour removal efficiency	813:842	true colour removal efficiency of 80%	813:849	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	4	29	theme	toxicity	591:598	arg1	tests					600:604	acute toxicity tests	585:604	acute toxicity tests	585:604	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	6	30	theme	%	891:891	arg1	values					803:808	Mean values	798:808	Mean values of true colour removal efficiency of 80%	798:849	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	6	30	theme	%	891:891	arg1	efficiency					873:882	turbidity removal efficiency	855:882	turbidity removal efficiency of 91.4%	855:891	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	7	31	theme	acute	957:961	arg1	tests					972:976	The acute toxicity tests	953:976	The acute toxicity tests	953:976	The acute toxicity tests showed that organisms were sensitive to all samples, mainly after coagulation-flocculation using chitosan.
27387003	5	32	theme	appropriate	775:785	arg1	coagulant					787:795	the most appropriate coagulant	766:795	the most appropriate coagulant	766:795	The analytic hierarchy process was employed for selecting the most appropriate coagulant.
27387003	1	33	theme	sanitary	223:230	arg1	leachate					241:248	sanitary landfill leachate	223:248	sanitary landfill leachate	223:248	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	0	34	theme	landfill	27:34	arg1	leachate					36:43	sanitary landfill leachate	18:43	sanitary landfill leachate	18:43	Post-treatment of sanitary landfill leachate by coagulation-flocculation using chitosan as primary coagulant.
27387003	10	35	theme	leachate	1449:1456	arg1	post-treatment					1422:1435	the post-treatment	1418:1435	the post-treatment of landfill leachate	1418:1456	Therefore, chitosan as primary coagulant was not considered to be a viable alternative in the post-treatment of landfill leachate.
27387003	4	36	theme	fish	664:667	arg1	reticulata					678:687	the guppy fish Poecilia reticulata	654:687	the guppy fish Poecilia reticulata	654:687	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	36	theme	fish	664:667	arg1	organisms					697:705	test organisms	692:705	test organisms	692:705	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	36	theme	fish	664:667	arg1	Lemna					638:642	the duckweed Lemna	625:642	the duckweed Lemna minor	625:648	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	1	37	theme	landfill	232:239	arg1	leachate					241:248	sanitary landfill leachate	223:248	sanitary landfill leachate	223:248	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	0	38	theme	sanitary	18:25	arg1	leachate					36:43	sanitary landfill leachate	18:43	sanitary landfill leachate	18:43	Post-treatment of sanitary landfill leachate by coagulation-flocculation using chitosan as primary coagulant.
27387003	3	39	contain	carried	488:494	arg2	tests					500:504	tests	500:504	tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O)	500:556	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	3	39	contain	carried	488:494	arg1	we					480:481	we	480:481	we	480:481	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	2	40	theme	surface	387:393	arg1	methodology					395:405	response surface methodology	378:405	response surface methodology	378:405	In order to optimize the process conditions, central composite design and response surface methodology were applied.
27387003	1	41	theme	leachate	241:248	arg1	treatment					210:218	a complementary coagulation-flocculation treatment	169:218	a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter	169:301	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	3	42	theme	H	551:551	arg1	sulphate					521:528	aluminium sulphate	511:528	aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O)	511:556	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	3	42	theme	H	551:551	arg1	O					555:555	Al(2) (SO(4))(3).14 H(2)O	531:555	Al(2) (SO(4))(3).14 H(2)O	531:555	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	4	43	theme	acute	585:589	arg1	tests					600:604	acute toxicity tests	585:604	acute toxicity tests	585:604	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	3	44	dep	O	555:555	arg1	2					553:553	2	553:553	2	553:553	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	0	45	theme	leachate	36:43	arg1	Post-treatment					0:13	Post-treatment	0:13	Post-treatment of sanitary landfill leachate by coagulation-flocculation using chitosan as primary coagulant.	0:108	Post-treatment of sanitary landfill leachate by coagulation-flocculation using chitosan as primary coagulant.
27387003	6	46	theme	removal	865:871	arg1	efficiency					873:882	turbidity removal efficiency	855:882	turbidity removal efficiency of 91.4%	855:891	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	3	47	theme	process	456:462	arg1	performance					437:447	the performance	433:447	the performance of the process using chitosan	433:477	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	8	48	theme	P.	1218:1219	arg1	reticulata					1221:1230	P. reticulata	1218:1230	P. reticulata	1218:1230	CE(50) for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC(50) for P. reticulata was 23% (v v(-1)).
27387003	6	49	theme	L	936:936	arg1	dosages					918:924	chitosan dosages	909:924	chitosan dosages of 960 mg L(-1)	909:940	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	8	50	dep	%	1238:1238	arg1	-1					1245:1246	-1	1245:1246	-1	1245:1246	CE(50) for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC(50) for P. reticulata was 23% (v v(-1)).
27387003	8	50	dep	%	1238:1238	arg1	v					1243:1243	v v	1241:1243	v v(-1)	1241:1247	CE(50) for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC(50) for P. reticulata was 23% (v v(-1)).
27387003	6	51	theme	turbidity	855:863	arg1	efficiency					873:882	turbidity removal efficiency	855:882	turbidity removal efficiency of 91.4%	855:891	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	6	52	theme	Mean	798:801	arg1	values					803:808	Mean values	798:808	Mean values of true colour removal efficiency of 80%	798:849	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	2	53	theme	response	378:385	arg1	methodology					395:405	response surface methodology	378:405	response surface methodology	378:405	In order to optimize the process conditions, central composite design and response surface methodology were applied.
27387003	3	54	with	tests	500:504	arg1	sulphate					521:528	aluminium sulphate	511:528	aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O)	511:556	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	3	54	with	tests	500:504	arg1	O					555:555	Al(2) (SO(4))(3).14 H(2)O	531:555	Al(2) (SO(4))(3).14 H(2)O	531:555	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	10	55	theme	primary	1351:1357	arg1	coagulant					1359:1367	primary coagulant	1351:1367	primary coagulant	1351:1367	Therefore, chitosan as primary coagulant was not considered to be a viable alternative in the post-treatment of landfill leachate.
27387003	6	56	theme	%	849:849	arg1	efficiency					833:842	true colour removal efficiency	813:842	true colour removal efficiency of 80%	813:849	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	9	57	theme	appropriate	1305:1315	arg1	coagulant					1317:1325	the most appropriate coagulant	1296:1325	the most appropriate coagulant	1296:1325	Multi-criteria analysis showed that alum was the most appropriate coagulant.
27387003	9	57	theme	appropriate	1305:1315	arg1	alum					1287:1290	alum	1287:1290	alum	1287:1290	Multi-criteria analysis showed that alum was the most appropriate coagulant.
27387003	1	58	theme	alternative	136:146	arg1	Chitosan					110:117	Chitosan	110:117	Chitosan	110:117	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	1	58	theme	alternative	136:146	arg1	coagulant					156:164	an alternative primary coagulant	133:164	an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter	133:301	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	6	59	theme	mg	933:934	arg1	-1					938:939	-1	938:939	-1	938:939	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	6	59	theme	mg	933:934	arg1	L					936:936	960 mg L	929:936	960 mg L(-1)	929:940	Mean values of true colour removal efficiency of 80% and turbidity removal efficiency of 91.4% were reached at chitosan dosages of 960 mg L(-1) at pH 8.5.
27387003	1	60	with	treatment	210:218	arg1	aim					259:261	the aim	255:261	the aim of removing recalcitrant organic matter	255:301	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	4	61	theme	guppy	658:662	arg1	reticulata					678:687	the guppy fish Poecilia reticulata	654:687	the guppy fish Poecilia reticulata	654:687	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	61	theme	guppy	658:662	arg1	organisms					697:705	test organisms	692:705	test organisms	692:705	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	61	theme	guppy	658:662	arg1	Lemna					638:642	the duckweed Lemna	625:642	the duckweed Lemna minor	625:648	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	2	62	theme	composite	357:365	arg1	design					367:372	central composite design	349:372	central composite design	349:372	In order to optimize the process conditions, central composite design and response surface methodology were applied.
27387003	4	63	theme	minor	644:648	arg1	reticulata					678:687	the guppy fish Poecilia reticulata	654:687	the guppy fish Poecilia reticulata	654:687	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	63	theme	minor	644:648	arg1	organisms					697:705	test organisms	692:705	test organisms	692:705	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	4	63	theme	minor	644:648	arg1	Lemna					638:642	the duckweed Lemna	625:642	the duckweed Lemna minor	625:648	In addition, acute toxicity tests were carried using the duckweed Lemna minor and the guppy fish Poecilia reticulata as test organisms.
27387003	10	64	theme	viable	1396:1401	arg1	chitosan					1339:1346	chitosan	1339:1346	chitosan as primary coagulant	1339:1367	Therefore, chitosan as primary coagulant was not considered to be a viable alternative in the post-treatment of landfill leachate.
27387003	10	64	theme	viable	1396:1401	arg1	alternative					1403:1413	a viable alternative	1394:1413	a viable alternative	1394:1413	Therefore, chitosan as primary coagulant was not considered to be a viable alternative in the post-treatment of landfill leachate.
27387003	8	65	theme	growth	1171:1176	arg1	rate					1178:1181	the average growth rate	1159:1181	the average growth rate	1159:1181	CE(50) for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC(50) for P. reticulata was 23% (v v(-1)).
27387003	3	66	theme	aluminium	511:519	arg1	sulphate					521:528	aluminium sulphate	511:528	aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O)	511:556	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	3	66	theme	aluminium	511:519	arg1	O					555:555	Al(2) (SO(4))(3).14 H(2)O	531:555	Al(2) (SO(4))(3).14 H(2)O	531:555	To evaluate the performance of the process using chitosan, we also carried out tests with aluminium sulphate (Al(2) (SO(4))(3).14 H(2)O) as coagulant.
27387003	1	67	theme	removing	266:273	arg1	matter					296:301	removing recalcitrant organic matter	266:301	removing recalcitrant organic matter	266:301	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
27387003	8	68	theme	v	1241:1241	arg1	-1					1245:1246	-1	1245:1246	-1	1245:1246	CE(50) for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC(50) for P. reticulata was 23% (v v(-1)).
27387003	8	68	theme	v	1241:1241	arg1	v					1243:1243	v v	1241:1243	v v(-1)	1241:1247	CE(50) for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC(50) for P. reticulata was 23% (v v(-1)).
27387003	8	69	theme	average	1163:1169	arg1	rate					1178:1181	the average growth rate	1159:1181	the average growth rate	1159:1181	CE(50) for L. minor was not determined because there was no inhibition of the average growth rate and biomass production; LC(50) for P. reticulata was 23% (v v(-1)).
27387003	1	70	theme	recalcitrant	275:286	arg1	matter					296:301	removing recalcitrant organic matter	266:301	removing recalcitrant organic matter	266:301	Chitosan was chosen as an alternative primary coagulant in a complementary coagulation-flocculation treatment of sanitary landfill leachate with the aim of removing recalcitrant organic matter.
26092170	4	0	theme	maximum	879:885	arg1	removal					896:902	maximum fluoride removal	879:902	maximum fluoride removal	879:902	There are various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for maximum fluoride removal.
26092170	3	1	with	SEM	695:697	arg1	techniques					709:718	EDAX techniques	704:718	EDAX techniques	704:718	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR, XRD and SEM with EDAX techniques.
26092170	2	2	theme	@	346:346	arg1	biocomposite					356:367	The synthesized Fe3O4@n-HApAlg biocomposite	325:367	The synthesized Fe3O4@n-HApAlg biocomposite	325:367	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	5	3	dep	Freundlich	946:955	arg1	isotherms					1006:1014	isotherms	1006:1014	isotherms	1006:1014	The equilibrium data was well modeled by Freundlich, Langmuir, Dubinin-Radushkevich (D-R) and Temkin isotherms.
26092170	8	4	theme	adsorbent	1235:1243	arg1	material					1245:1252	the adsorbent material	1231:1252	the adsorbent material	1231:1252	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	2	5	theme	Fe3O4	341:345	arg1	biocomposite					356:367	The synthesized Fe3O4@n-HApAlg biocomposite	325:367	The synthesized Fe3O4@n-HApAlg biocomposite	325:367	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	1	6	theme	composite	254:262	arg1	nano-hydroxyapatite					212:230	nano-hydroxyapatite	212:230	nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite)	212:289	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	1	6	theme	composite	254:262	arg1	n-HAp					232:236	n-HAp	232:236	n-HAp	232:236	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	2	7	theme	n-HAp	478:482	arg1	composite					484:492	Fe3O4@n-HAp composite	472:492	Fe3O4@n-HAp composite	472:492	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	3	8	theme	structural	592:601	arg1	changes					603:609	The structural changes	588:609	The structural changes of the sorbent, before and after fluoride sorption	588:660	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR, XRD and SEM with EDAX techniques.
26092170	4	9	theme	contact	775:781	arg1	time					783:786	contact time	775:786	contact time	775:786	There are various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for maximum fluoride removal.
26092170	1	10	from	fabrication	169:179	arg1	nano-hydroxyapatite					212:230	nano-hydroxyapatite	212:230	nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite)	212:289	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	1	10	from	fabrication	169:179	arg1	n-HAp					232:236	n-HAp	232:236	n-HAp	232:236	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	8	11	theme	water	1272:1276	arg1	samples					1278:1284	water samples	1272:1284	water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu	1272:1394	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	2	12	theme	@	477:477	arg1	composite					484:492	Fe3O4@n-HAp composite	472:492	Fe3O4@n-HAp composite	472:492	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	1	13	from	defluoridation	295:308	arg1	mode					319:322	batch mode	313:322	batch mode	313:322	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	2	14	theme	mgF	564:566	arg1	/kg					570:572	3870, 2469, 1296 and 1050 mgF(-)/kg	538:572	3870, 2469, 1296 and 1050 mgF(-)/kg	538:572	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	7	15	theme	endothermic	1156:1166	arg1	nature					1168:1173	endothermic nature	1156:1173	endothermic nature	1156:1173	The thermodynamic parameters reveals that the feasibility, spontaneity and endothermic nature of fluoride sorption.
26092170	1	16	theme	Fe3O4	265:269	arg1	composite					280:288	Fe3O4@n-HApAlg composite	265:288	Fe3O4@n-HApAlg composite	265:288	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	2	17	theme	Fe3O4	472:476	arg1	composite					484:492	Fe3O4@n-HAp composite	472:492	Fe3O4@n-HAp composite	472:492	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	2	18	theme	synthesized	329:339	arg1	biocomposite					356:367	The synthesized Fe3O4@n-HApAlg biocomposite	325:367	The synthesized Fe3O4@n-HApAlg biocomposite	325:367	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	3	19	with	FTIR	681:684	arg1	techniques					709:718	EDAX techniques	704:718	EDAX techniques	704:718	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR, XRD and SEM with EDAX techniques.
26092170	2	20	theme	defluoridation	389:402	arg1	DC					414:415	DC	414:415	DC	414:415	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	2	20	theme	defluoridation	389:402	arg1	capacity					404:411	an enhanced defluoridation capacity	377:411	an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively	377:585	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	3	21	theme	EDAX	704:707	arg1	techniques					709:718	EDAX techniques	704:718	EDAX techniques	704:718	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR, XRD and SEM with EDAX techniques.
26092170	7	22	theme	thermodynamic	1085:1097	arg1	parameters					1099:1108	The thermodynamic parameters	1081:1108	The thermodynamic parameters	1081:1108	The thermodynamic parameters reveals that the feasibility, spontaneity and endothermic nature of fluoride sorption.
26092170	1	23	theme	magnetic	184:191	arg1	particles					199:207	magnetic Fe3O4 particles	184:207	magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite)	184:289	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	3	24	theme	sorbent	618:624	arg1	changes					603:609	The structural changes	588:609	The structural changes of the sorbent, before and after fluoride sorption	588:660	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR, XRD and SEM with EDAX techniques.
26092170	4	25	theme	physico-chemical	739:754	arg1	parameters					756:765	various physico-chemical parameters	731:765	various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for maximum fluoride removal	731:902	There are various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for maximum fluoride removal.
26092170	3	26	theme	fluoride	644:651	arg1	sorption					653:660	fluoride sorption	644:660	fluoride sorption	644:660	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR, XRD and SEM with EDAX techniques.
26092170	1	27	theme	research	106:113	arg1	study					115:119	this research study	101:119	this research study	101:119	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	1	28	theme	Fe3O4	193:197	arg1	particles					199:207	magnetic Fe3O4 particles	184:207	magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite)	184:289	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	2	29	theme	mgF	426:428	arg1	/kg					432:434	4050 mgF(-)/kg	421:434	4050 mgF(-)/kg	421:434	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	1	30	theme	@	270:270	arg1	composite					280:288	Fe3O4@n-HApAlg composite	265:288	Fe3O4@n-HApAlg composite	265:288	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	4	31	theme	various	731:737	arg1	parameters					756:765	various physico-chemical parameters	731:765	various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for maximum fluoride removal	731:902	There are various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for maximum fluoride removal.
26092170	2	32	theme	/kg	570:572	arg1	DCs					531:533	the DCs	527:533	the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg	527:572	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	6	33	theme	Dubinin-Radushkevich	1044:1063	arg1	model					1074:1078	Dubinin-Radushkevich isotherm model	1044:1078	Dubinin-Radushkevich isotherm model	1044:1078	The present system follows Dubinin-Radushkevich isotherm model.
26092170	1	34	theme	particles	199:207	arg1	fabrication					169:179	the fabrication	165:179	the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite)	165:289	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	0	35	theme	facile	28:33	arg1	separation					35:44	facile separation	28:44	facile separation	28:44	Enhanced defluoridation and facile separation of magnetic nano-hydroxyapatite/alginate composite.
26092170	1	36	theme	n-HApAlg	271:278	arg1	composite					280:288	Fe3O4@n-HApAlg composite	265:288	Fe3O4@n-HApAlg composite	265:288	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	3	37	with	XRD	687:689	arg1	techniques					709:718	EDAX techniques	704:718	EDAX techniques	704:718	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR, XRD and SEM with EDAX techniques.
26092170	4	38	theme	co-existing	793:803	arg1	anions					805:810	co-existing anions	793:810	co-existing anions	793:810	There are various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for maximum fluoride removal.
26092170	2	39	contain	possess	369:375	arg2	DC					414:415	DC	414:415	DC	414:415	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	2	39	contain	possess	369:375	arg2	capacity					404:411	an enhanced defluoridation capacity	377:411	an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively	377:585	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	2	39	contain	possess	369:375	arg1	biocomposite					356:367	The synthesized Fe3O4@n-HApAlg biocomposite	325:367	The synthesized Fe3O4@n-HApAlg biocomposite	325:367	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	8	40	theme	endemic	1310:1316	arg1	Ammapatti					1351:1359	Ammapatti	1351:1359	Ammapatti	1351:1359	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	8	40	theme	endemic	1310:1316	arg1	Reddiyarchatram					1331:1345	Reddiyarchatram	1331:1345	Reddiyarchatram	1331:1345	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	8	40	theme	endemic	1310:1316	arg1	areas					1318:1322	fluoride endemic areas	1301:1322	fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu	1301:1394	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	2	41	contain	possesses	517:525	arg1	n-HAp					495:499	n-HAp	495:499	n-HAp	495:499	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	2	41	contain	possesses	517:525	arg2	DCs					531:533	the DCs	527:533	the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg	527:572	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	2	41	contain	possesses	517:525	arg1	composite					484:492	Fe3O4@n-HAp composite	472:492	Fe3O4@n-HAp composite	472:492	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	2	41	contain	possesses	517:525	arg1	composite					461:469	n-HApAlg composite	452:469	n-HApAlg composite	452:469	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	2	41	contain	possesses	517:525	arg1	Fe3O4					505:509	Fe3O4	505:509	Fe3O4	505:509	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	4	42	theme	initial	813:819	arg1	concentration					830:842	initial fluoride concentration	813:842	initial fluoride concentration	813:842	There are various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for maximum fluoride removal.
26092170	5	43	theme	equilibrium	909:919	arg1	data					921:924	The equilibrium data	905:924	The equilibrium data	905:924	The equilibrium data was well modeled by Freundlich, Langmuir, Dubinin-Radushkevich (D-R) and Temkin isotherms.
26092170	8	44	dep	areas	1318:1322	arg1	Ammapatti					1351:1359	Ammapatti	1351:1359	Ammapatti	1351:1359	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	8	44	dep	areas	1318:1322	arg1	Reddiyarchatram					1331:1345	Reddiyarchatram	1331:1345	Reddiyarchatram	1331:1345	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	8	44	dep	areas	1318:1322	arg1	areas					1318:1322	fluoride endemic areas	1301:1322	fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu	1301:1394	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	6	45	theme	isotherm	1065:1072	arg1	model					1074:1078	Dubinin-Radushkevich isotherm model	1044:1078	Dubinin-Radushkevich isotherm model	1044:1078	The present system follows Dubinin-Radushkevich isotherm model.
26092170	2	46	theme	/kg	432:434	arg1	DC					414:415	DC	414:415	DC	414:415	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	2	46	theme	/kg	432:434	arg1	capacity					404:411	an enhanced defluoridation capacity	377:411	an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively	377:585	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	4	47	theme	fluoride	887:894	arg1	removal					896:902	maximum fluoride removal	879:902	maximum fluoride removal	879:902	There are various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for maximum fluoride removal.
26092170	8	48	theme	standard	1402:1409	arg1	protocols					1411:1419	standard protocols	1402:1419	standard protocols	1402:1419	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	2	49	dep	capacity	404:411	arg1	compare					441:447	compare	441:447	compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively	441:585	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	0	50	theme	nano-hydroxyapatite/alginate	58:85	arg1	composite					87:95	magnetic nano-hydroxyapatite/alginate composite	49:95	magnetic nano-hydroxyapatite/alginate composite	49:95	Enhanced defluoridation and facile separation of magnetic nano-hydroxyapatite/alginate composite.
26092170	8	51	theme	fluoride	1301:1308	arg1	Ammapatti					1351:1359	Ammapatti	1351:1359	Ammapatti	1351:1359	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	8	51	theme	fluoride	1301:1308	arg1	Reddiyarchatram					1331:1345	Reddiyarchatram	1331:1345	Reddiyarchatram	1331:1345	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	8	51	theme	fluoride	1301:1308	arg1	areas					1318:1322	fluoride endemic areas	1301:1322	fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu	1301:1394	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	2	52	theme	n-HApAlg	452:459	arg1	composite					461:469	n-HApAlg composite	452:469	n-HApAlg composite	452:469	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	1	53	theme	new	124:126	arg1	biosorbent					137:146	a new magnetic biosorbent	122:146	a new magnetic biosorbent	122:146	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	0	54	theme	magnetic	49:56	arg1	composite					87:95	magnetic nano-hydroxyapatite/alginate composite	49:95	magnetic nano-hydroxyapatite/alginate composite	49:95	Enhanced defluoridation and facile separation of magnetic nano-hydroxyapatite/alginate composite.
26092170	1	55	theme	magnetic	128:135	arg1	biosorbent					137:146	a new magnetic biosorbent	122:146	a new magnetic biosorbent	122:146	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	1	56	theme	batch	313:317	arg1	mode					319:322	batch mode	313:322	batch mode	313:322	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	1	57	from	nano-hydroxyapatite	212:230	arg1	fabrication					169:179	the fabrication	165:179	the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite)	165:289	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	7	58	theme	sorption	1187:1194	arg1	spontaneity					1140:1150	spontaneity	1140:1150	spontaneity	1140:1150	The thermodynamic parameters reveals that the feasibility, spontaneity and endothermic nature of fluoride sorption.
26092170	7	58	theme	sorption	1187:1194	arg1	nature					1168:1173	endothermic nature	1156:1173	endothermic nature	1156:1173	The thermodynamic parameters reveals that the feasibility, spontaneity and endothermic nature of fluoride sorption.
26092170	7	58	theme	sorption	1187:1194	arg1	feasibility					1127:1137	the feasibility	1123:1137	the feasibility	1123:1137	The thermodynamic parameters reveals that the feasibility, spontaneity and endothermic nature of fluoride sorption.
26092170	8	59	theme	Nadu	1391:1394	arg1	Ammapatti					1351:1359	Ammapatti	1351:1359	Ammapatti	1351:1359	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	8	59	theme	Nadu	1391:1394	arg1	Reddiyarchatram					1331:1345	Reddiyarchatram	1331:1345	Reddiyarchatram	1331:1345	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	8	59	theme	Nadu	1391:1394	arg1	areas					1318:1322	fluoride endemic areas	1301:1322	fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu	1301:1394	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	8	60	dep	performance	1201:1211	arg1	The					1197:1199	The	1197:1199	The	1197:1199	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	7	61	theme	fluoride	1178:1185	arg1	sorption					1187:1194	fluoride sorption	1178:1194	fluoride sorption	1178:1194	The thermodynamic parameters reveals that the feasibility, spontaneity and endothermic nature of fluoride sorption.
26092170	6	62	theme	present	1021:1027	arg1	system					1029:1034	The present system	1017:1034	The present system	1017:1034	The present system follows Dubinin-Radushkevich isotherm model.
26092170	1	63	dep	composite	254:262	arg1	composite					280:288	Fe3O4@n-HApAlg composite	265:288	Fe3O4@n-HApAlg composite	265:288	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	1	64	from	particles	199:207	arg1	nano-hydroxyapatite					212:230	nano-hydroxyapatite	212:230	nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite)	212:289	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	1	64	from	particles	199:207	arg1	n-HAp					232:236	n-HAp	232:236	n-HAp	232:236	In this research study, a new magnetic biosorbent was developed by the fabrication of magnetic Fe3O4 particles on nano-hydroxyapatite(n-HAp)/alginate (Alg) composite (Fe3O4@n-HApAlg composite) for defluoridation in batch mode.
26092170	8	65	theme	material	1245:1252	arg1	efficiency					1217:1226	efficiency	1217:1226	efficiency	1217:1226	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	8	65	theme	material	1245:1252	arg1	performance					1201:1211	performance	1201:1211	performance	1201:1211	The performance and efficiency of the adsorbent material was examined with water samples collected from fluoride endemic areas namely Reddiyarchatram and Ammapatti in Dindigul District of Tamil Nadu using standard protocols.
26092170	2	66	theme	enhanced	380:387	arg1	DC					414:415	DC	414:415	DC	414:415	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	2	66	theme	enhanced	380:387	arg1	capacity					404:411	an enhanced defluoridation capacity	377:411	an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively	377:585	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	0	67	theme	composite	87:95	arg1	defluoridation					9:22	defluoridation	9:22	defluoridation	9:22	Enhanced defluoridation and facile separation of magnetic nano-hydroxyapatite/alginate composite.
26092170	0	67	theme	composite	87:95	arg1	separation					35:44	facile separation	28:44	facile separation	28:44	Enhanced defluoridation and facile separation of magnetic nano-hydroxyapatite/alginate composite.
26092170	2	68	theme	n-HApAlg	347:354	arg1	biocomposite					356:367	The synthesized Fe3O4@n-HApAlg biocomposite	325:367	The synthesized Fe3O4@n-HApAlg biocomposite	325:367	The synthesized Fe3O4@n-HApAlg biocomposite possess an enhanced defluoridation capacity (DC) of 4050 mgF(-)/kg when compare to n-HApAlg composite, Fe3O4@n-HAp composite, n-HAp and Fe3O4 which possesses the DCs of 3870, 2469, 1296 and 1050 mgF(-)/kg respectively.
26092170	4	69	theme	fluoride	821:828	arg1	concentration					830:842	initial fluoride concentration	813:842	initial fluoride concentration	813:842	There are various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for maximum fluoride removal.
25622232	7	0	theme	surface	1283:1289	arg1	coefficient					1300:1310	the surface friction coefficient	1279:1310	the surface friction coefficient of the polyorganosiloxane network	1279:1344	Moreover, starch granules considerably lowered the surface friction coefficient of the polyorganosiloxane network.
25622232	2	1	theme	dimensional	360:370	arg1	stability					372:380	its poor dimensional stability	351:380	its poor dimensional stability under humid and dry conditions	351:411	Although thermoplastic (destructured) starch has emerged as an alternative to petroleum-based polymers, its poor dimensional stability under humid and dry conditions extensively hinders its use as the biopolymer of choice in many applications.
25622232	4	2	theme	mechanically	895:906	arg1	bioelastomers					928:940	mechanically robust, hydrophobic bioelastomers	895:940	mechanically robust, hydrophobic bioelastomers	895:940	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	9	3	used	used	1694:1697	arg2	chemistry					1676:1684	addition-cured polysiloxane chemistry	1648:1684	addition-cured polysiloxane chemistry that is used extensively in microfabrication	1648:1729	Corn starch granules also have excellent compatibility with addition-cured polysiloxane chemistry that is used extensively in microfabrication.
25622232	6	4	theme	elastic	1208:1214	arg1	rates					1225:1229	high elastic recovery rates	1203:1229	high elastic recovery rates	1203:1229	Depending on the amount of starch granules, the mechanical properties of the bioelastomers can be easily tuned with high elastic recovery rates.
25622232	10	5	theme	water	1880:1884	arg1	capacity					1893:1900	much less water uptake capacity	1870:1900	much less water uptake capacity	1870:1900	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	1	6	theme	good	193:196	arg1	resistance					198:207	good resistance	193:207	good resistance to water	193:216	Designing starch-based biopolymers and biodegradable composites with durable mechanical properties and good resistance to water is still a challenging task.
25622232	10	7	contain	have	1807:1810	arg2	surfaces					1824:1831	hydrophobic surfaces	1812:1831	hydrophobic surfaces with lower friction coefficients and much less water uptake capacity	1812:1900	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	10	7	contain	have	1807:1810	arg1	all					1772:1774	all	1772:1774	all	1772:1774	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	10	7	contain	have	1807:1810	arg1	bioelastomers					1793:1805	the developed bioelastomers	1779:1805	the developed bioelastomers	1779:1805	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	3	8	from	granules	509:516	arg1	hand					532:535	the other hand	522:535	the other hand	522:535	Unmodified starch granules, on the other hand, suffer from incompatibility, poor dispersion, and phase separation issues when compounded into other thermoplastics above a concentration level of 5%.
25622232	10	9	theme	thermoplastic	1916:1928	arg1	starch					1930:1935	thermoplastic starch	1916:1935	thermoplastic starch	1916:1935	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	3	10	theme	poor	567:570	arg1	dispersion					572:581	poor dispersion	567:581	poor dispersion	567:581	Unmodified starch granules, on the other hand, suffer from incompatibility, poor dispersion, and phase separation issues when compounded into other thermoplastics above a concentration level of 5%.
25622232	10	11	theme	starch	1750:1755	arg1	concentration					1757:1769	the starch concentration	1746:1769	the starch concentration	1746:1769	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	8	12	theme	relaxation	1354:1363	arg1	measurements					1365:1376	Stress relaxation measurements	1347:1376	Stress relaxation measurements	1347:1376	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	3	13	theme	phase	588:592	arg1	separation					594:603	phase separation	588:603	phase separation	588:603	Unmodified starch granules, on the other hand, suffer from incompatibility, poor dispersion, and phase separation issues when compounded into other thermoplastics above a concentration level of 5%.
25622232	9	14	theme	Corn	1588:1591	arg1	granules					1600:1607	Corn starch granules	1588:1607	Corn starch granules	1588:1607	Corn starch granules also have excellent compatibility with addition-cured polysiloxane chemistry that is used extensively in microfabrication.
25622232	8	15	theme	plastic	1551:1557	arg1	dampeners					1577:1585	plastic mechanical energy dampeners	1551:1585	plastic mechanical energy dampeners	1551:1585	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	4	16	theme	biodegradable	717:729	arg1	method					753:758	a facile biodegradable elastomer preparation method	708:758	a facile biodegradable elastomer preparation method	708:758	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	3	17	theme	other	633:637	arg1	thermoplastics					639:652	other thermoplastics	633:652	other thermoplastics above a concentration level of 5%	633:686	Unmodified starch granules, on the other hand, suffer from incompatibility, poor dispersion, and phase separation issues when compounded into other thermoplastics above a concentration level of 5%.
25622232	3	18	dep	incompatibility	550:564	arg1	issues					605:610	issues	605:610	issues	605:610	Unmodified starch granules, on the other hand, suffer from incompatibility, poor dispersion, and phase separation issues when compounded into other thermoplastics above a concentration level of 5%.
25622232	1	19	theme	biodegradable	129:141	arg1	composites					143:152	biodegradable composites	129:152	biodegradable composites	129:152	Designing starch-based biopolymers and biodegradable composites with durable mechanical properties and good resistance to water is still a challenging task.
25622232	8	20	theme	energy	1570:1575	arg1	dampeners					1577:1585	plastic mechanical energy dampeners	1551:1585	plastic mechanical energy dampeners	1551:1585	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	2	21	theme	thermoplastic	256:268	arg1	starch					285:290	thermoplastic (destructured) starch	256:290	thermoplastic (destructured) starch	256:290	Although thermoplastic (destructured) starch has emerged as an alternative to petroleum-based polymers, its poor dimensional stability under humid and dry conditions extensively hinders its use as the biopolymer of choice in many applications.
25622232	1	22	theme	durable	159:165	arg1	properties					178:187	durable mechanical properties	159:187	durable mechanical properties	159:187	Designing starch-based biopolymers and biodegradable composites with durable mechanical properties and good resistance to water is still a challenging task.
25622232	4	23	dep	robust	908:913	arg1	hydrophobic					916:926	hydrophobic	916:926	hydrophobic	916:926	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	2	24	theme	destructured	271:282	arg1	starch					285:290	thermoplastic (destructured) starch	256:290	thermoplastic (destructured) starch	256:290	Although thermoplastic (destructured) starch has emerged as an alternative to petroleum-based polymers, its poor dimensional stability under humid and dry conditions extensively hinders its use as the biopolymer of choice in many applications.
25622232	4	25	theme	large	777:781	arg1	starch					810:815	unmodified corn starch	794:815	unmodified corn starch	794:815	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	4	25	theme	large	777:781	arg1	amounts					783:789	large amounts	777:789	large amounts of unmodified corn starch	777:815	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	7	26	theme	starch	1242:1247	arg1	granules					1249:1256	starch granules	1242:1256	starch granules	1242:1256	Moreover, starch granules considerably lowered the surface friction coefficient of the polyorganosiloxane network.
25622232	6	27	theme	recovery	1216:1223	arg1	rates					1225:1229	high elastic recovery rates	1203:1229	high elastic recovery rates	1203:1229	Depending on the amount of starch granules, the mechanical properties of the bioelastomers can be easily tuned with high elastic recovery rates.
25622232	5	28	theme	starch	993:998	arg1	surface					982:988	the surface	978:988	the surface of starch	978:998	The naturally adsorbed moisture on the surface of starch enables autocatalytic rapid hydrolysis of polyorganosiloxane to form Si-O-Si networks.
25622232	5	29	theme	adsorbed	957:964	arg1	moisture					966:973	The naturally adsorbed moisture	943:973	The naturally adsorbed moisture on the surface of starch	943:998	The naturally adsorbed moisture on the surface of starch enables autocatalytic rapid hydrolysis of polyorganosiloxane to form Si-O-Si networks.
25622232	5	30	theme	autocatalytic	1008:1020	arg1	hydrolysis					1028:1037	autocatalytic rapid hydrolysis	1008:1037	autocatalytic rapid hydrolysis of polyorganosiloxane	1008:1059	The naturally adsorbed moisture on the surface of starch enables autocatalytic rapid hydrolysis of polyorganosiloxane to form Si-O-Si networks.
25622232	6	31	theme	starch	1114:1119	arg1	granules					1121:1128	starch granules	1114:1128	starch granules	1114:1128	Depending on the amount of starch granules, the mechanical properties of the bioelastomers can be easily tuned with high elastic recovery rates.
25622232	2	32	theme	choice	462:467	arg1	biopolymer					448:457	the biopolymer	444:457	the biopolymer of choice in many applications	444:488	Although thermoplastic (destructured) starch has emerged as an alternative to petroleum-based polymers, its poor dimensional stability under humid and dry conditions extensively hinders its use as the biopolymer of choice in many applications.
25622232	4	33	theme	preparation	741:751	arg1	method					753:758	a facile biodegradable elastomer preparation method	708:758	a facile biodegradable elastomer preparation method	708:758	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	2	34	theme	dry	398:400	arg1	conditions					402:411	humid and dry conditions	388:411	humid and dry conditions	388:411	Although thermoplastic (destructured) starch has emerged as an alternative to petroleum-based polymers, its poor dimensional stability under humid and dry conditions extensively hinders its use as the biopolymer of choice in many applications.
25622232	0	35	theme	Robust	0:5	arg1	elastomers					25:34	Robust and biodegradable elastomers	0:34	Robust and biodegradable elastomers	0:34	Robust and biodegradable elastomers based on corn starch and polydimethylsiloxane (PDMS).
25622232	10	36	theme	hydrophobic	1812:1822	arg1	surfaces					1824:1831	hydrophobic surfaces	1812:1831	hydrophobic surfaces with lower friction coefficients and much less water uptake capacity	1812:1900	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	0	37	theme	biodegradable	11:23	arg1	elastomers					25:34	Robust and biodegradable elastomers	0:34	Robust and biodegradable elastomers	0:34	Robust and biodegradable elastomers based on corn starch and polydimethylsiloxane (PDMS).
25622232	1	38	theme	starch-based	100:111	arg1	biopolymers					113:123	starch-based biopolymers	100:123	starch-based biopolymers	100:123	Designing starch-based biopolymers and biodegradable composites with durable mechanical properties and good resistance to water is still a challenging task.
25622232	3	39	theme	concentration	662:674	arg1	level					676:680	a concentration level	660:680	a concentration level of 5%	660:686	Unmodified starch granules, on the other hand, suffer from incompatibility, poor dispersion, and phase separation issues when compounded into other thermoplastics above a concentration level of 5%.
25622232	4	40	dep	produce	887:893	arg1	acetoxy-polyorganosiloxane					846:871	acetoxy-polyorganosiloxane	846:871	acetoxy-polyorganosiloxane	846:871	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	2	41	theme	many	472:475	arg1	applications					477:488	many applications	472:488	many applications	472:488	Although thermoplastic (destructured) starch has emerged as an alternative to petroleum-based polymers, its poor dimensional stability under humid and dry conditions extensively hinders its use as the biopolymer of choice in many applications.
25622232	10	42	theme	friction	1844:1851	arg1	coefficients					1853:1864	lower friction coefficients	1838:1864	lower friction coefficients	1838:1864	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	0	43	theme	corn	45:48	arg1	starch					50:55	corn starch	45:55	corn starch	45:55	Robust and biodegradable elastomers based on corn starch and polydimethylsiloxane (PDMS).
25622232	8	44	dep	green	1529:1533	arg1	substitutes					1535:1545	substitutes	1535:1545	substitutes	1535:1545	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	6	45	theme	bioelastomers	1164:1176	arg1	properties					1146:1155	the mechanical properties	1131:1155	the mechanical properties of the bioelastomers	1131:1176	Depending on the amount of starch granules, the mechanical properties of the bioelastomers can be easily tuned with high elastic recovery rates.
25622232	9	46	contain	have	1614:1617	arg2	compatibility					1629:1641	excellent compatibility	1619:1641	excellent compatibility	1619:1641	Corn starch granules also have excellent compatibility with addition-cured polysiloxane chemistry that is used extensively in microfabrication.
25622232	9	46	contain	have	1614:1617	arg1	granules					1600:1607	Corn starch granules	1588:1607	Corn starch granules	1588:1607	Corn starch granules also have excellent compatibility with addition-cured polysiloxane chemistry that is used extensively in microfabrication.
25622232	3	47	theme	%	686:686	arg1	level					676:680	a concentration level	660:680	a concentration level of 5%	660:686	Unmodified starch granules, on the other hand, suffer from incompatibility, poor dispersion, and phase separation issues when compounded into other thermoplastics above a concentration level of 5%.
25622232	4	48	theme	corn	805:808	arg1	starch					810:815	unmodified corn starch	794:815	unmodified corn starch	794:815	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	3	49	theme	Unmodified	491:500	arg1	granules					509:516	Unmodified starch granules	491:516	Unmodified starch granules	491:516	Unmodified starch granules, on the other hand, suffer from incompatibility, poor dispersion, and phase separation issues when compounded into other thermoplastics above a concentration level of 5%.
25622232	1	50	theme	challenging	229:239	arg1	task					241:244	still a challenging task	221:244	still a challenging task	221:244	Designing starch-based biopolymers and biodegradable composites with durable mechanical properties and good resistance to water is still a challenging task.
25622232	7	51	theme	polyorganosiloxane	1319:1336	arg1	network					1338:1344	the polyorganosiloxane network	1315:1344	the polyorganosiloxane network	1315:1344	Moreover, starch granules considerably lowered the surface friction coefficient of the polyorganosiloxane network.
25622232	3	52	theme	other	526:530	arg1	hand					532:535	the other hand	522:535	the other hand	522:535	Unmodified starch granules, on the other hand, suffer from incompatibility, poor dispersion, and phase separation issues when compounded into other thermoplastics above a concentration level of 5%.
25622232	5	53	theme	polyorganosiloxane	1042:1059	arg1	hydrolysis					1028:1037	autocatalytic rapid hydrolysis	1008:1037	autocatalytic rapid hydrolysis of polyorganosiloxane	1008:1059	The naturally adsorbed moisture on the surface of starch enables autocatalytic rapid hydrolysis of polyorganosiloxane to form Si-O-Si networks.
25622232	6	54	theme	mechanical	1135:1144	arg1	properties					1146:1155	the mechanical properties	1131:1155	the mechanical properties of the bioelastomers	1131:1176	Depending on the amount of starch granules, the mechanical properties of the bioelastomers can be easily tuned with high elastic recovery rates.
25622232	7	55	theme	friction	1291:1298	arg1	coefficient					1300:1310	the surface friction coefficient	1279:1310	the surface friction coefficient of the polyorganosiloxane network	1279:1344	Moreover, starch granules considerably lowered the surface friction coefficient of the polyorganosiloxane network.
25622232	9	56	theme	excellent	1619:1627	arg1	compatibility					1629:1641	excellent compatibility	1619:1641	excellent compatibility	1619:1641	Corn starch granules also have excellent compatibility with addition-cured polysiloxane chemistry that is used extensively in microfabrication.
25622232	2	57	theme	poor	355:358	arg1	stability					372:380	its poor dimensional stability	351:380	its poor dimensional stability under humid and dry conditions	351:411	Although thermoplastic (destructured) starch has emerged as an alternative to petroleum-based polymers, its poor dimensional stability under humid and dry conditions extensively hinders its use as the biopolymer of choice in many applications.
25622232	10	58	theme	uptake	1886:1891	arg1	capacity					1893:1900	much less water uptake capacity	1870:1900	much less water uptake capacity	1870:1900	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	6	59	theme	high	1203:1206	arg1	rates					1225:1229	high elastic recovery rates	1203:1229	high elastic recovery rates	1203:1229	Depending on the amount of starch granules, the mechanical properties of the bioelastomers can be easily tuned with high elastic recovery rates.
25622232	2	60	theme	petroleum-based	325:339	arg1	polymers					341:348	petroleum-based polymers	325:348	petroleum-based polymers	325:348	Although thermoplastic (destructured) starch has emerged as an alternative to petroleum-based polymers, its poor dimensional stability under humid and dry conditions extensively hinders its use as the biopolymer of choice in many applications.
25622232	8	61	contain	have	1411:1414	arg2	factors					1442:1448	strain energy dissipation factors	1416:1448	strain energy dissipation factors that are lower than those of conventional rubbers	1416:1498	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	8	61	contain	have	1411:1414	arg1	bioelastomers					1397:1409	the bioelastomers	1393:1409	the bioelastomers	1393:1409	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	11	62	theme	Mediterranean	2009:2021	arg1	seawater					2023:2030	Mediterranean seawater	2009:2030	Mediterranean seawater	2009:2030	The bioelastomers are biocompatible and are estimated to biodegrade in Mediterranean seawater within three to six years.
25622232	4	63	theme	robust	908:913	arg1	bioelastomers					928:940	mechanically robust, hydrophobic bioelastomers	895:940	mechanically robust, hydrophobic bioelastomers	895:940	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	8	64	theme	mechanical	1559:1568	arg1	dampeners					1577:1585	plastic mechanical energy dampeners	1551:1585	plastic mechanical energy dampeners	1551:1585	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	8	65	theme	Stress	1347:1352	arg1	measurements					1365:1376	Stress relaxation measurements	1347:1376	Stress relaxation measurements	1347:1376	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	9	66	theme	starch	1593:1598	arg1	granules					1600:1607	Corn starch granules	1588:1607	Corn starch granules	1588:1607	Corn starch granules also have excellent compatibility with addition-cured polysiloxane chemistry that is used extensively in microfabrication.
25622232	8	67	theme	energy	1423:1428	arg1	factors					1442:1448	strain energy dissipation factors	1416:1448	strain energy dissipation factors that are lower than those of conventional rubbers	1416:1498	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	4	68	theme	facile	710:715	arg1	method					753:758	a facile biodegradable elastomer preparation method	708:758	a facile biodegradable elastomer preparation method	708:758	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	2	69	from	biopolymer	448:457	arg1	applications					477:488	many applications	472:488	many applications	472:488	Although thermoplastic (destructured) starch has emerged as an alternative to petroleum-based polymers, its poor dimensional stability under humid and dry conditions extensively hinders its use as the biopolymer of choice in many applications.
25622232	1	70	theme	mechanical	167:176	arg1	properties					178:187	durable mechanical properties	159:187	durable mechanical properties	159:187	Designing starch-based biopolymers and biodegradable composites with durable mechanical properties and good resistance to water is still a challenging task.
25622232	2	71	theme	humid	388:392	arg1	conditions					402:411	humid and dry conditions	388:411	humid and dry conditions	388:411	Although thermoplastic (destructured) starch has emerged as an alternative to petroleum-based polymers, its poor dimensional stability under humid and dry conditions extensively hinders its use as the biopolymer of choice in many applications.
25622232	9	72	theme	addition-cured	1648:1661	arg1	chemistry					1676:1684	addition-cured polysiloxane chemistry	1648:1684	addition-cured polysiloxane chemistry that is used extensively in microfabrication	1648:1729	Corn starch granules also have excellent compatibility with addition-cured polysiloxane chemistry that is used extensively in microfabrication.
25622232	8	73	theme	strain	1416:1421	arg1	factors					1442:1448	strain energy dissipation factors	1416:1448	strain energy dissipation factors that are lower than those of conventional rubbers	1416:1498	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	9	74	theme	polysiloxane	1663:1674	arg1	chemistry					1676:1684	addition-cured polysiloxane chemistry	1648:1684	addition-cured polysiloxane chemistry that is used extensively in microfabrication	1648:1729	Corn starch granules also have excellent compatibility with addition-cured polysiloxane chemistry that is used extensively in microfabrication.
25622232	6	75	theme	granules	1121:1128	arg1	amount					1104:1109	the amount	1100:1109	the amount of starch granules	1100:1128	Depending on the amount of starch granules, the mechanical properties of the bioelastomers can be easily tuned with high elastic recovery rates.
25622232	6	75	theme	granules	1121:1128	arg1	granules					1121:1128	starch granules	1114:1128	starch granules	1114:1128	Depending on the amount of starch granules, the mechanical properties of the bioelastomers can be easily tuned with high elastic recovery rates.
25622232	8	76	theme	dissipation	1430:1440	arg1	factors					1442:1448	strain energy dissipation factors	1416:1448	strain energy dissipation factors that are lower than those of conventional rubbers	1416:1498	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	4	77	theme	elastomer	731:739	arg1	method					753:758	a facile biodegradable elastomer preparation method	708:758	a facile biodegradable elastomer preparation method	708:758	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	10	78	theme	less	1875:1878	arg1	capacity					1893:1900	much less water uptake capacity	1870:1900	much less water uptake capacity	1870:1900	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	10	79	with	surfaces	1824:1831	arg1	capacity					1893:1900	much less water uptake capacity	1870:1900	much less water uptake capacity	1870:1900	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	10	79	with	surfaces	1824:1831	arg1	coefficients					1853:1864	lower friction coefficients	1838:1864	lower friction coefficients	1838:1864	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	11	80	dep	six	2048:2050	arg1	to					2045:2046	to	2045:2046	to	2045:2046	The bioelastomers are biocompatible and are estimated to biodegrade in Mediterranean seawater within three to six years.
25622232	10	81	theme	developed	1783:1791	arg1	bioelastomers					1793:1805	the developed bioelastomers	1779:1805	the developed bioelastomers	1779:1805	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	5	82	theme	Si-O-Si	1069:1075	arg1	networks					1077:1084	Si-O-Si networks	1069:1084	Si-O-Si networks	1069:1084	The naturally adsorbed moisture on the surface of starch enables autocatalytic rapid hydrolysis of polyorganosiloxane to form Si-O-Si networks.
25622232	7	83	theme	network	1338:1344	arg1	coefficient					1300:1310	the surface friction coefficient	1279:1310	the surface friction coefficient of the polyorganosiloxane network	1279:1344	Moreover, starch granules considerably lowered the surface friction coefficient of the polyorganosiloxane network.
25622232	8	84	theme	conventional	1479:1490	arg1	rubbers					1492:1498	conventional rubbers	1479:1498	conventional rubbers	1479:1498	Stress relaxation measurements indicated that the bioelastomers have strain energy dissipation factors that are lower than those of conventional rubbers, rendering them as promising green substitutes for plastic mechanical energy dampeners.
25622232	10	85	theme	lower	1838:1842	arg1	coefficients					1853:1864	lower friction coefficients	1838:1864	lower friction coefficients	1838:1864	Regardless of the starch concentration, all of the developed bioelastomers have hydrophobic surfaces with lower friction coefficients and much less water uptake capacity than those of thermoplastic starch.
25622232	5	86	theme	rapid	1022:1026	arg1	hydrolysis					1028:1037	autocatalytic rapid hydrolysis	1008:1037	autocatalytic rapid hydrolysis of polyorganosiloxane	1008:1059	The naturally adsorbed moisture on the surface of starch enables autocatalytic rapid hydrolysis of polyorganosiloxane to form Si-O-Si networks.
25622232	4	87	theme	unmodified	794:803	arg1	starch					810:815	unmodified corn starch	794:815	unmodified corn starch	794:815	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	3	88	theme	starch	502:507	arg1	granules					509:516	Unmodified starch granules	491:516	Unmodified starch granules	491:516	Unmodified starch granules, on the other hand, suffer from incompatibility, poor dispersion, and phase separation issues when compounded into other thermoplastics above a concentration level of 5%.
25622232	5	89	from	moisture	966:973	arg1	surface					982:988	the surface	978:988	the surface of starch	978:998	The naturally adsorbed moisture on the surface of starch enables autocatalytic rapid hydrolysis of polyorganosiloxane to form Si-O-Si networks.
25622232	4	90	theme	starch	810:815	arg1	starch					810:815	unmodified corn starch	794:815	unmodified corn starch	794:815	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
25622232	4	90	theme	starch	810:815	arg1	amounts					783:789	large amounts	777:789	large amounts of unmodified corn starch	777:815	Herein, we present a facile biodegradable elastomer preparation method by incorporating large amounts of unmodified corn starch, exceeding 80% by volume, in acetoxy-polyorganosiloxane thermosets to produce mechanically robust, hydrophobic bioelastomers.
24127303	0	0	theme	Ganoderma	94:102	arg1	lucidum					104:110	Ganoderma lucidum	94:110	Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes	94:165	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	4	1	theme	&	889:889	arg1	NMR					900:902	(1)H & (13)C 1D NMR	884:902	(1)H & (13)C 1D NMR	884:902	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	5	2	dep	PTP1B	1075:1079	arg1	potency					1103:1109	potency	1103:1109	potency	1103:1109	The chemical structure of FYGL-a was determined as following, which may play special role in the competitive inhibition of PTP1B and antihyperglycemia potency.
24127303	1	3	theme	water-soluble	170:182	arg1	inhibitor					190:198	A water-soluble PTP1B inhibitor	168:198	A water-soluble PTP1B inhibitor	168:198	A water-soluble PTP1B inhibitor, named FYGL-a, was fractionated for structure investigation and bioactivity evaluation.
24127303	0	4	from	characterization	11:26	arg1	bodies					121:126	Ganoderma lucidum fruiting bodies	94:126	Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes	94:165	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	4	5	theme	H	887:887	arg1	NMR					900:902	(1)H & (13)C 1D NMR	884:902	(1)H & (13)C 1D NMR	884:902	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	1	6	theme	PTP1B	184:188	arg1	inhibitor					190:198	A water-soluble PTP1B inhibitor	168:198	A water-soluble PTP1B inhibitor	168:198	A water-soluble PTP1B inhibitor, named FYGL-a, was fractionated for structure investigation and bioactivity evaluation.
24127303	0	7	theme	fruiting	112:119	arg1	bodies					121:126	Ganoderma lucidum fruiting bodies	94:126	Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes	94:165	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	3	8	theme	O-linkage	715:723	arg1	type					725:728	O-linkage type	715:728	O-linkage type	715:728	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	9	from	bonded	677:682	arg1	type					725:728	O-linkage type	715:728	O-linkage type	715:728	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	10	theme	100.2	441:445	arg1	kDa					447:449	kDa	447:449	kDa	447:449	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	0	11	theme	lucidum	104:110	arg1	bodies					121:126	Ganoderma lucidum fruiting bodies	94:126	Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes	94:165	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	2	12	from	bodies	384:389	arg1	FYGL-a					288:293	FYGL-a	288:293	FYGL-a	288:293	FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies.
24127303	2	12	from	bodies	384:389	arg1	extract					344:350	a reported antihyperglycemia extract	315:350	a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies	315:389	FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies.
24127303	2	12	from	bodies	384:389	arg1	ingredient					301:310	an ingredient	298:310	an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies	298:389	FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies.
24127303	4	13	theme	complete	758:765	arg1	sequence					767:774	The complete sequence	754:774	The complete sequence of FYGL-a	754:784	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	3	14	theme	acid	571:574	arg1	residues					576:583	glucuronic acid residues	560:583	glucuronic acid residues	560:583	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	15	theme	Composition	392:402	arg1	analysis					404:411	Composition analysis	392:411	Composition analysis	392:411	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	16	theme	threonine	734:742	arg1	residues					744:751	threonine residues	734:751	threonine residues	734:751	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	4	17	theme	&	942:942	arg1	TOCSY					944:948	& TOCSY	942:948	& TOCSY	942:948	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	4	17	theme	&	942:942	arg1	HMBC					923:926	HMBC	923:926	HMBC	923:926	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	3	18	theme	85 ± 2	486:491	arg1	%					492:492	%	492:492	%	492:492	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	0	19	from	evaluation	44:53	arg1	bodies					121:126	Ganoderma lucidum fruiting bodies	94:126	Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes	94:165	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	4	20	theme	FYGL-a	779:784	arg1	sequence					767:774	The complete sequence	754:774	The complete sequence of FYGL-a	754:784	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	0	21	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	2	22	theme	extract	344:350	arg1	FYGL-a					288:293	FYGL-a	288:293	FYGL-a	288:293	FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies.
24127303	2	22	theme	extract	344:350	arg1	ingredient					301:310	an ingredient	298:310	an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies	298:389	FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies.
24127303	0	23	theme	bioactivity	32:42	arg1	evaluation					44:53	bioactivity evaluation	32:53	bioactivity evaluation	32:53	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	4	24	theme	Smith	843:847	arg1	degradation					849:859	Smith degradation	843:859	Smith degradation	843:859	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	2	25	theme	antihyperglycemia	326:342	arg1	extract					344:350	a reported antihyperglycemia extract	315:350	a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies	315:389	FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies.
24127303	3	26	theme	1.0:3.7:3.9:2.0	604:618	arg1	ratio					595:599	a mole ratio	588:599	a mole ratio of 1.0:3.7:3.9:2.0	588:618	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	0	27	theme	PTP1B	132:136	arg1	inhibition					138:147	PTP1B inhibition	132:147	PTP1B inhibition	132:147	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	2	28	theme	reported	317:324	arg1	extract					344:350	a reported antihyperglycemia extract	315:350	a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies	315:389	FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies.
24127303	3	29	theme	acidic	451:456	arg1	FYGL-a					428:433	FYGL-a	428:433	FYGL-a	428:433	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	29	theme	acidic	451:456	arg1	proteoglycan					458:469	a 100.2 kDa acidic proteoglycan	439:469	a 100.2 kDa acidic proteoglycan	439:469	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	30	with	chain	515:519	arg1	galactose					536:544	galactose	536:544	galactose	536:544	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	30	with	chain	515:519	arg1	rhamnose					526:533	rhamnose	526:533	rhamnose	526:533	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	30	with	chain	515:519	arg1	residues					576:583	glucuronic acid residues	560:583	glucuronic acid residues	560:583	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	30	with	chain	515:519	arg1	glucose					547:553	glucose	547:553	glucose	547:553	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	31	theme	%	492:492	arg1	chain					515:519	85 ± 2% heteropolysaccharide chain	486:519	85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues	486:583	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	4	32	theme	2D	909:910	arg1	NMR					912:914	2D NMR	909:914	2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY)	909:949	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	3	33	theme	FYGL-a	655:660	arg1	bonded					677:682	bonded	677:682	bonded	677:682	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	33	theme	FYGL-a	655:660	arg1	moiety					645:650	the 15 ± 2% protein moiety	625:650	the 15 ± 2% protein moiety of FYGL-a	625:660	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	34	theme	15 ± 2	629:634	arg1	%					635:635	%	635:635	%	635:635	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	35	theme	heteropolysaccharide	494:513	arg1	chain					515:519	85 ± 2% heteropolysaccharide chain	486:519	85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues	486:583	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	0	36	theme	acidic	61:66	arg1	extract					81:87	an acidic proteoglycan extract	58:87	an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes	58:165	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	4	37	dep	NMR	912:914	arg1	HSQC					917:920	HSQC	917:920	HSQC	917:920	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	4	38	theme	C	895:895	arg1	NMR					900:902	(1)H & (13)C 1D NMR	884:902	(1)H & (13)C 1D NMR	884:902	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	5	39	theme	FYGL-a	978:983	arg1	following					1003:1011	following	1003:1011	following	1003:1011	The chemical structure of FYGL-a was determined as following, which may play special role in the competitive inhibition of PTP1B and antihyperglycemia potency.
24127303	5	39	theme	FYGL-a	978:983	arg1	structure					965:973	The chemical structure	952:973	The chemical structure of FYGL-a	952:983	The chemical structure of FYGL-a was determined as following, which may play special role in the competitive inhibition of PTP1B and antihyperglycemia potency.
24127303	5	40	theme	chemical	956:963	arg1	following					1003:1011	following	1003:1011	following	1003:1011	The chemical structure of FYGL-a was determined as following, which may play special role in the competitive inhibition of PTP1B and antihyperglycemia potency.
24127303	5	40	theme	chemical	956:963	arg1	structure					965:973	The chemical structure	952:973	The chemical structure of FYGL-a	952:983	The chemical structure of FYGL-a was determined as following, which may play special role in the competitive inhibition of PTP1B and antihyperglycemia potency.
24127303	3	41	from	type	725:728	arg1	bonded					677:682	bonded	677:682	bonded	677:682	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	41	from	type	725:728	arg1	moiety					645:650	the 15 ± 2% protein moiety	625:650	the 15 ± 2% protein moiety of FYGL-a	625:660	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	5	42	theme	competitive	1049:1059	arg1	inhibition					1061:1070	the competitive inhibition	1045:1070	the competitive inhibition of PTP1B and antihyperglycemia potency	1045:1109	The chemical structure of FYGL-a was determined as following, which may play special role in the competitive inhibition of PTP1B and antihyperglycemia potency.
24127303	3	43	theme	kDa	447:449	arg1	FYGL-a					428:433	FYGL-a	428:433	FYGL-a	428:433	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	43	theme	kDa	447:449	arg1	proteoglycan					458:469	a 100.2 kDa acidic proteoglycan	439:469	a 100.2 kDa acidic proteoglycan	439:469	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	44	theme	polysaccharide	691:704	arg1	chain					706:710	the polysaccharide chain	687:710	the polysaccharide chain	687:710	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	4	45	theme	methylation	862:872	arg1	analysis					874:881	methylation analysis	862:881	methylation analysis	862:881	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	2	46	theme	fruiting	375:382	arg1	bodies					384:389	Ganoderma Lucidum fruiting bodies	357:389	Ganoderma Lucidum fruiting bodies	357:389	FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies.
24127303	2	47	from	ingredient	301:310	arg1	bodies					384:389	Ganoderma Lucidum fruiting bodies	357:389	Ganoderma Lucidum fruiting bodies	357:389	FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies.
24127303	5	48	theme	antihyperglycemia	1085:1101	arg1	inhibition					1061:1070	the competitive inhibition	1045:1070	the competitive inhibition of PTP1B and antihyperglycemia potency	1045:1109	The chemical structure of FYGL-a was determined as following, which may play special role in the competitive inhibition of PTP1B and antihyperglycemia potency.
24127303	0	49	from	bodies	121:126	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	0	49	from	bodies	121:126	arg1	evaluation					44:53	bioactivity evaluation	32:53	bioactivity evaluation	32:53	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	0	49	from	bodies	121:126	arg1	extract					81:87	an acidic proteoglycan extract	58:87	an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes	58:165	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	3	50	from	proteoglycan	458:469	arg1	ratio					595:599	a mole ratio	588:599	a mole ratio of 1.0:3.7:3.9:2.0	588:618	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	2	51	theme	Lucidum	367:373	arg1	bodies					384:389	Ganoderma Lucidum fruiting bodies	357:389	Ganoderma Lucidum fruiting bodies	357:389	FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies.
24127303	3	52	theme	mole	590:593	arg1	ratio					595:599	a mole ratio	588:599	a mole ratio of 1.0:3.7:3.9:2.0	588:618	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	1	53	theme	structure	236:244	arg1	investigation					246:258	structure investigation	236:258	structure investigation	236:258	A water-soluble PTP1B inhibitor, named FYGL-a, was fractionated for structure investigation and bioactivity evaluation.
24127303	4	54	theme	1D	897:898	arg1	NMR					900:902	(1)H & (13)C 1D NMR	884:902	(1)H & (13)C 1D NMR	884:902	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	4	55	theme	periodate	822:830	arg1	oxidation					832:840	periodate oxidation	822:840	periodate oxidation	822:840	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	2	56	theme	Ganoderma	357:365	arg1	Lucidum					367:373	Ganoderma Lucidum	357:373	Ganoderma Lucidum fruiting bodies	357:389	FYGL-a is an ingredient of a reported antihyperglycemia extract from Ganoderma Lucidum fruiting bodies.
24127303	4	57	dep	HSQC	917:920	arg1	TOCSY					944:948	& TOCSY	942:948	& TOCSY	942:948	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	4	57	dep	HSQC	917:920	arg1	NOESY					929:933	NOESY	929:933	NOESY	929:933	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	4	57	dep	HSQC	917:920	arg1	HMBC					923:926	HMBC	923:926	HMBC	923:926	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	4	57	dep	HSQC	917:920	arg1	COSY					936:939	COSY	936:939	COSY	936:939	The complete sequence of FYGL-a was characterized systematically by periodate oxidation, Smith degradation, methylation analysis, (1)H & (13)C 1D NMR, and 2D NMR (HSQC, HMBC, NOESY, COSY, & TOCSY).
24127303	0	58	theme	extract	81:87	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	0	58	theme	extract	81:87	arg1	evaluation					44:53	bioactivity evaluation	32:53	bioactivity evaluation	32:53	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	3	59	theme	%	635:635	arg1	bonded					677:682	bonded	677:682	bonded	677:682	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	59	theme	%	635:635	arg1	moiety					645:650	the 15 ± 2% protein moiety	625:650	the 15 ± 2% protein moiety of FYGL-a	625:660	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	0	60	theme	proteoglycan	68:79	arg1	extract					81:87	an acidic proteoglycan extract	58:87	an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes	58:165	Structural characterization and bioactivity evaluation of an acidic proteoglycan extract from Ganoderma lucidum fruiting bodies for PTP1B inhibition and anti-diabetes.
24127303	5	61	theme	PTP1B	1075:1079	arg1	inhibition					1061:1070	the competitive inhibition	1045:1070	the competitive inhibition of PTP1B and antihyperglycemia potency	1045:1109	The chemical structure of FYGL-a was determined as following, which may play special role in the competitive inhibition of PTP1B and antihyperglycemia potency.
24127303	3	62	theme	protein	637:643	arg1	bonded					677:682	bonded	677:682	bonded	677:682	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	3	62	theme	protein	637:643	arg1	moiety					645:650	the 15 ± 2% protein moiety	625:650	the 15 ± 2% protein moiety of FYGL-a	625:660	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	5	63	theme	special	1029:1035	arg1	role					1037:1040	special role	1029:1040	special role	1029:1040	The chemical structure of FYGL-a was determined as following, which may play special role in the competitive inhibition of PTP1B and antihyperglycemia potency.
24127303	3	64	theme	glucuronic	560:569	arg1	residues					576:583	glucuronic acid residues	560:583	glucuronic acid residues	560:583	Composition analysis indicated that FYGL-a was a 100.2 kDa acidic proteoglycan, consisting of 85 ± 2% heteropolysaccharide chain with rhamnose, galactose, glucose, and glucuronic acid residues in a mole ratio of 1.0:3.7:3.9:2.0, and the 15 ± 2% protein moiety of FYGL-a was covalently bonded to the polysaccharide chain in O-linkage type via threonine residues.
24127303	1	65	theme	bioactivity	264:274	arg1	evaluation					276:285	bioactivity evaluation	264:285	bioactivity evaluation	264:285	A water-soluble PTP1B inhibitor, named FYGL-a, was fractionated for structure investigation and bioactivity evaluation.
27885831	4	0	theme	stable	667:672	arg1	network					714:720	a stable, ultraporous and hydrophillic nanofiber network	665:720	a stable, ultraporous and hydrophillic nanofiber network	665:720	To create the fibrillar component, cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network.
27885831	2	1	theme	embedded	354:361	arg1	cells					325:329	cells	325:329	cells	325:329	Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork.
27885831	5	2	theme	fiber	879:883	arg1	mesh					885:888	the fiber mesh	875:888	the fiber mesh	875:888	In this study, cell-laden, fiber-reinforced composite scaffolds thicker than 1.5 mm can be created by infiltrating a chondrocyte/alginate solution into the fiber mesh, which is then physically cross-linked.
27885831	5	3	theme	composite	767:775	arg1	scaffolds					777:785	fiber-reinforced composite scaffolds	750:785	fiber-reinforced composite scaffolds thicker than 1.5 mm	750:805	In this study, cell-laden, fiber-reinforced composite scaffolds thicker than 1.5 mm can be created by infiltrating a chondrocyte/alginate solution into the fiber mesh, which is then physically cross-linked.
27885831	5	3	theme	composite	767:775	arg1	cell-laden					738:747	cell-laden	738:747	cell-laden	738:747	In this study, cell-laden, fiber-reinforced composite scaffolds thicker than 1.5 mm can be created by infiltrating a chondrocyte/alginate solution into the fiber mesh, which is then physically cross-linked.
27885831	7	4	theme	II-rich	1121:1127	arg1	matrix					1129:1134	a glycosaminoglycan- and collagen type II-rich matrix	1082:1134	matrix	1129:1134	This allows the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite in vivo.
27885831	6	5	theme	chondroinductive	983:998	arg1	hydrogels					1041:1049	the chondroinductive, but mechanically weak sulfated alginate hydrogels	979:1049	the chondroinductive, but mechanically weak sulfated alginate hydrogels	979:1049	The fibrillar component significantly reinforces the chondroinductive, but mechanically weak sulfated alginate hydrogels.
27885831	7	6	theme	matrix	1129:1134	arg1	production					1068:1077	the production	1064:1077	the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite	1064:1191	This allows the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite in vivo.
27885831	7	6	theme	matrix	1129:1134	arg1	survival					1167:1174	survival	1167:1174	the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite	1064:1191	This allows the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite in vivo.
27885831	8	7	theme	inflammatory	1338:1349	arg1	insult					1351:1356	an IL-1β-mediated inflammatory insult	1320:1356	an IL-1β-mediated inflammatory insult	1320:1356	To further enhance the system, the electrospun component is loaded with dexamethasone, which protected the cells from an IL-1β-mediated inflammatory insult.
27885831	3	8	theme	ECM	507:509	arg1	component					483:491	the glycosaminoglycan component	461:491	the glycosaminoglycan component of the native ECM	461:509	Here, a polyanionic functionalized alginate is used to mimic the glycosaminoglycan component of the native ECM.
27885831	2	9	theme	fibrillar	335:343	arg1	proteins					345:352	fibrillar proteins	335:352	fibrillar proteins	335:352	Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork.
27885831	7	10	theme	collagen	1107:1114	arg1	matrix					1129:1134	a glycosaminoglycan- and collagen type II-rich matrix	1082:1134	matrix	1129:1134	This allows the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite in vivo.
27885831	2	11	theme	Articular	275:283	arg1	cartilage					285:293	Articular cartilage	275:293	Articular cartilage	275:293	Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork.
27885831	2	11	theme	Articular	275:283	arg1	tissue					310:315	a composite tissue	298:315	a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork	298:397	Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork.
27885831	3	12	theme	functionalized	420:433	arg1	alginate					435:442	a polyanionic functionalized alginate	406:442	a polyanionic functionalized alginate	406:442	Here, a polyanionic functionalized alginate is used to mimic the glycosaminoglycan component of the native ECM.
27885831	5	13	theme	fiber-reinforced	750:765	arg1	scaffolds					777:785	fiber-reinforced composite scaffolds	750:785	fiber-reinforced composite scaffolds thicker than 1.5 mm	750:805	In this study, cell-laden, fiber-reinforced composite scaffolds thicker than 1.5 mm can be created by infiltrating a chondrocyte/alginate solution into the fiber mesh, which is then physically cross-linked.
27885831	5	13	theme	fiber-reinforced	750:765	arg1	cell-laden					738:747	cell-laden	738:747	cell-laden	738:747	In this study, cell-laden, fiber-reinforced composite scaffolds thicker than 1.5 mm can be created by infiltrating a chondrocyte/alginate solution into the fiber mesh, which is then physically cross-linked.
27885831	7	14	theme	type	1116:1119	arg1	matrix					1129:1134	a glycosaminoglycan- and collagen type II-rich matrix	1082:1134	matrix	1129:1134	This allows the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite in vivo.
27885831	2	15	from	embedded	354:361	arg1	meshwork					390:397	a hydrophilic polymeric meshwork	366:397	a hydrophilic polymeric meshwork	366:397	Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork.
27885831	6	16	theme	sulfated	1023:1030	arg1	hydrogels					1041:1049	the chondroinductive, but mechanically weak sulfated alginate hydrogels	979:1049	the chondroinductive, but mechanically weak sulfated alginate hydrogels	979:1049	The fibrillar component significantly reinforces the chondroinductive, but mechanically weak sulfated alginate hydrogels.
27885831	0	17	theme	Bioinspired	2:12	arg1	Nanofiber-Hydrogel					26:43	A Bioinspired Ultraporous Nanofiber-Hydrogel	0:43	A Bioinspired Ultraporous Nanofiber-Hydrogel	0:43	A Bioinspired Ultraporous Nanofiber-Hydrogel Mimic of the Cartilage Extracellular Matrix.
27885831	4	18	theme	poly	570:573	arg1	cryoelectrospinning					547:565	cryoelectrospinning	547:565	cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma,	547:645	To create the fibrillar component, cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network.
27885831	7	19	theme	glycosaminoglycan-	1084:1101	arg1	production					1068:1077	the production	1064:1077	the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite	1064:1191	This allows the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite in vivo.
27885831	7	19	theme	glycosaminoglycan-	1084:1101	arg1	survival					1167:1174	survival	1167:1174	the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite	1064:1191	This allows the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite in vivo.
27885831	2	20	theme	polymeric	380:388	arg1	meshwork					390:397	a hydrophilic polymeric meshwork	366:397	a hydrophilic polymeric meshwork	366:397	Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork.
27885831	6	21	theme	alginate	1032:1039	arg1	hydrogels					1041:1049	the chondroinductive, but mechanically weak sulfated alginate hydrogels	979:1049	the chondroinductive, but mechanically weak sulfated alginate hydrogels	979:1049	The fibrillar component significantly reinforces the chondroinductive, but mechanically weak sulfated alginate hydrogels.
27885831	4	22	theme	nanofiber	704:712	arg1	network					714:720	a stable, ultraporous and hydrophillic nanofiber network	665:720	a stable, ultraporous and hydrophillic nanofiber network	665:720	To create the fibrillar component, cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network.
27885831	2	23	theme	hydrophilic	368:378	arg1	meshwork					390:397	a hydrophilic polymeric meshwork	366:397	a hydrophilic polymeric meshwork	366:397	Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork.
27885831	2	24	theme	composite	300:308	arg1	cartilage					285:293	Articular cartilage	275:293	Articular cartilage	275:293	Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork.
27885831	2	24	theme	composite	300:308	arg1	tissue					310:315	a composite tissue	298:315	a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork	298:397	Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork.
27885831	0	25	theme	Ultraporous	14:24	arg1	Nanofiber-Hydrogel					26:43	A Bioinspired Ultraporous Nanofiber-Hydrogel	0:43	A Bioinspired Ultraporous Nanofiber-Hydrogel	0:43	A Bioinspired Ultraporous Nanofiber-Hydrogel Mimic of the Cartilage Extracellular Matrix.
27885831	3	26	used	used	447:450	arg2	alginate					435:442	a polyanionic functionalized alginate	406:442	a polyanionic functionalized alginate	406:442	Here, a polyanionic functionalized alginate is used to mimic the glycosaminoglycan component of the native ECM.
27885831	3	27	theme	glycosaminoglycan	465:481	arg1	component					483:491	the glycosaminoglycan component	461:491	the glycosaminoglycan component of the native ECM	461:509	Here, a polyanionic functionalized alginate is used to mimic the glycosaminoglycan component of the native ECM.
27885831	3	28	theme	native	500:505	arg1	ECM					507:509	the native ECM	496:509	the native ECM	496:509	Here, a polyanionic functionalized alginate is used to mimic the glycosaminoglycan component of the native ECM.
27885831	1	29	dep	demanding	236:244	arg1	inflamed					253:260	inflamed	253:260	inflamed	253:260	A true biomimetic of the cartilage extracellular matrix (ECM) could greatly contribute to our ability to regenerate this tissue in a mechanically demanding, often inflamed environment.
27885831	6	30	theme	fibrillar	934:942	arg1	component					944:952	The fibrillar component	930:952	The fibrillar component	930:952	The fibrillar component significantly reinforces the chondroinductive, but mechanically weak sulfated alginate hydrogels.
27885831	8	31	theme	electrospun	1237:1247	arg1	component					1249:1257	the electrospun component	1233:1257	the electrospun component	1233:1257	To further enhance the system, the electrospun component is loaded with dexamethasone, which protected the cells from an IL-1β-mediated inflammatory insult.
27885831	3	32	theme	polyanionic	408:418	arg1	alginate					435:442	a polyanionic functionalized alginate	406:442	a polyanionic functionalized alginate	406:442	Here, a polyanionic functionalized alginate is used to mimic the glycosaminoglycan component of the native ECM.
27885831	4	33	theme	fibrillar	526:534	arg1	component					536:544	the fibrillar component	522:544	the fibrillar component	522:544	To create the fibrillar component, cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network.
27885831	5	34	theme	chondrocyte/alginate	840:859	arg1	solution					861:868	a chondrocyte/alginate solution	838:868	a chondrocyte/alginate solution	838:868	In this study, cell-laden, fiber-reinforced composite scaffolds thicker than 1.5 mm can be created by infiltrating a chondrocyte/alginate solution into the fiber mesh, which is then physically cross-linked.
27885831	0	35	theme	Cartilage	58:66	arg1	Matrix					82:87	the Cartilage Extracellular Matrix	54:87	the Cartilage Extracellular Matrix	54:87	A Bioinspired Ultraporous Nanofiber-Hydrogel Mimic of the Cartilage Extracellular Matrix.
27885831	4	36	used	used	650:653	arg2	mandrel					603:609	a -78 °C mandrel	594:609	a -78 °C mandrel	594:609	To create the fibrillar component, cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network.
27885831	4	36	used	used	650:653	arg2	cryoelectrospinning					547:565	cryoelectrospinning	547:565	cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma,	547:645	To create the fibrillar component, cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network.
27885831	1	37	theme	cartilage	115:123	arg1	matrix					139:144	the cartilage extracellular matrix	111:144	the cartilage extracellular matrix (ECM)	111:150	A true biomimetic of the cartilage extracellular matrix (ECM) could greatly contribute to our ability to regenerate this tissue in a mechanically demanding, often inflamed environment.
27885831	1	37	theme	cartilage	115:123	arg1	ECM					147:149	ECM	147:149	ECM	147:149	A true biomimetic of the cartilage extracellular matrix (ECM) could greatly contribute to our ability to regenerate this tissue in a mechanically demanding, often inflamed environment.
27885831	1	38	theme	extracellular	125:137	arg1	matrix					139:144	the cartilage extracellular matrix	111:144	the cartilage extracellular matrix (ECM)	111:150	A true biomimetic of the cartilage extracellular matrix (ECM) could greatly contribute to our ability to regenerate this tissue in a mechanically demanding, often inflamed environment.
27885831	1	38	theme	extracellular	125:137	arg1	ECM					147:149	ECM	147:149	ECM	147:149	A true biomimetic of the cartilage extracellular matrix (ECM) could greatly contribute to our ability to regenerate this tissue in a mechanically demanding, often inflamed environment.
27885831	4	39	theme	ultraporous	675:685	arg1	network					714:720	a stable, ultraporous and hydrophillic nanofiber network	665:720	a stable, ultraporous and hydrophillic nanofiber network	665:720	To create the fibrillar component, cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network.
27885831	2	40	from	meshwork	390:397	arg1	embedded					354:361	embedded	354:361	embedded	354:361	Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork.
27885831	1	41	theme	matrix	139:144	arg1	biomimetic					97:106	biomimetic	97:106	biomimetic	97:106	A true biomimetic of the cartilage extracellular matrix (ECM) could greatly contribute to our ability to regenerate this tissue in a mechanically demanding, often inflamed environment.
27885831	4	42	theme	°C	600:601	arg1	mandrel					603:609	a -78 °C mandrel	594:609	a -78 °C mandrel	594:609	To create the fibrillar component, cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network.
27885831	8	43	theme	IL-1β-mediated	1323:1336	arg1	insult					1351:1356	an IL-1β-mediated inflammatory insult	1320:1356	an IL-1β-mediated inflammatory insult	1320:1356	To further enhance the system, the electrospun component is loaded with dexamethasone, which protected the cells from an IL-1β-mediated inflammatory insult.
27885831	4	44	theme	hydrophillic	691:702	arg1	network					714:720	a stable, ultraporous and hydrophillic nanofiber network	665:720	a stable, ultraporous and hydrophillic nanofiber network	665:720	To create the fibrillar component, cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network.
27885831	6	45	theme	weak	1018:1021	arg1	hydrogels					1041:1049	the chondroinductive, but mechanically weak sulfated alginate hydrogels	979:1049	the chondroinductive, but mechanically weak sulfated alginate hydrogels	979:1049	The fibrillar component significantly reinforces the chondroinductive, but mechanically weak sulfated alginate hydrogels.
27885831	0	46	theme	Extracellular	68:80	arg1	Matrix					82:87	the Cartilage Extracellular Matrix	54:87	the Cartilage Extracellular Matrix	54:87	A Bioinspired Ultraporous Nanofiber-Hydrogel Mimic of the Cartilage Extracellular Matrix.
27885831	4	47	from	cryoelectrospinning	547:565	arg1	mandrel					603:609	a -78 °C mandrel	594:609	a -78 °C mandrel	594:609	To create the fibrillar component, cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network.
27885831	5	48	theme	thicker	787:793	arg1	scaffolds					777:785	fiber-reinforced composite scaffolds	750:785	fiber-reinforced composite scaffolds thicker than 1.5 mm	750:805	In this study, cell-laden, fiber-reinforced composite scaffolds thicker than 1.5 mm can be created by infiltrating a chondrocyte/alginate solution into the fiber mesh, which is then physically cross-linked.
27885831	5	48	theme	thicker	787:793	arg1	cell-laden					738:747	cell-laden	738:747	cell-laden	738:747	In this study, cell-laden, fiber-reinforced composite scaffolds thicker than 1.5 mm can be created by infiltrating a chondrocyte/alginate solution into the fiber mesh, which is then physically cross-linked.
27885831	7	49	theme	composite	1183:1191	arg1	production					1068:1077	the production	1064:1077	the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite	1064:1191	This allows the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite in vivo.
27885831	7	49	theme	composite	1183:1191	arg1	survival					1167:1174	survival	1167:1174	the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite	1064:1191	This allows the production of a glycosaminoglycan- and collagen type II-rich matrix by the chondrocytes as well as survival of the composite in vivo.
27885831	1	50	theme	demanding	236:244	arg1	environment					262:272	a mechanically demanding, often inflamed environment	221:272	a mechanically demanding, often inflamed environment	221:272	A true biomimetic of the cartilage extracellular matrix (ECM) could greatly contribute to our ability to regenerate this tissue in a mechanically demanding, often inflamed environment.
26375986	0	0	theme	Inner	67:71	arg1	Bark					73:76	the Inner Bark	63:76	the Inner Bark of Quillaja saponaria Molina	63:105	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	5	1	theme	potential	943:951	arg1	function					953:960	their potential function	937:960	their potential function as substrate for enzymatic browning reactions	937:1006	Besides their antioxidant activity and their potential function as substrate for enzymatic browning reactions, the new compounds are highly characteristic for both the inner bark of Q. saponaria and commercial extracts derived therefrom.
26375986	4	2	theme	putative	777:784	arg1	pathway					799:805	a putative biosynthetic pathway	775:805	a putative biosynthetic pathway of at least 20 structurally related p-coumaroyl sucrose esters	775:868	In addition, a putative biosynthetic pathway of at least 20 structurally related p-coumaroyl sucrose esters was tentatively identified.
26375986	1	3	theme	prepared	144:151	arg1	extracts					161:168	freshly prepared aqueous extracts	136:168	freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina	136:215	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	3	4	theme	4-O-	677:680	arg1	-α-d-glup-					697:706	(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-	542:712	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	512:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	4	theme	4-O-	677:680	arg1	-p-coumaroyl					684:695	4-O-(E)-p-coumaroyl	677:695	4-O-(E)-p-coumaroyl	677:695	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	6	5	from	detection	1199:1207	arg1	food					1233:1236	food	1233:1236	food	1233:1236	Consequently, they might serve as authenticity markers for the detection of Quillaja extracts in food and cosmetic formulations.
26375986	6	5	from	detection	1199:1207	arg1	cosmetic					1242:1249	cosmetic	1242:1249	cosmetic	1242:1249	Consequently, they might serve as authenticity markers for the detection of Quillaja extracts in food and cosmetic formulations.
26375986	6	6	theme	extracts	1221:1228	arg1	detection					1199:1207	the detection	1195:1207	the detection of Quillaja extracts in food and cosmetic formulations	1195:1262	Consequently, they might serve as authenticity markers for the detection of Quillaja extracts in food and cosmetic formulations.
26375986	3	7	theme	1→4	656:658	arg1	-α-l-rhap-					660:669	(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-	579:675	-α-l-rhap-	660:669	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	8	theme	-p-coumaroyl	587:598	arg1	A					624:624	(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-	579:675	A	624:624	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	9	theme	quillajaside	745:756	arg1	-β-d-fruf					734:742	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf	512:742	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	512:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	9	theme	quillajaside	745:756	arg1	B					758:758	quillajaside B	745:758	quillajaside B	745:758	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	10	theme	-α-d-glup-	697:706	arg1	-β-d-fruf					734:742	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf	512:742	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	512:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	10	theme	-α-d-glup-	697:706	arg1	B					758:758	quillajaside B	745:758	quillajaside B	745:758	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	5	11	theme	enzymatic	979:987	arg1	reactions					998:1006	enzymatic browning reactions	979:1006	enzymatic browning reactions	979:1006	Besides their antioxidant activity and their potential function as substrate for enzymatic browning reactions, the new compounds are highly characteristic for both the inner bark of Q. saponaria and commercial extracts derived therefrom.
26375986	0	12	theme	saponaria	90:98	arg1	Molina					100:105	Quillaja saponaria Molina	81:105	Quillaja saponaria Molina	81:105	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	3	13	dep	isolated	484:491	arg1	characterized					497:509	characterized	497:509	characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	497:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	13	dep	isolated	484:491	arg1	compounds					469:477	two new compounds	461:477	two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	461:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	5	14	theme	new	1013:1015	arg1	compounds					1017:1025	the new compounds	1009:1025	the new compounds	1009:1025	Besides their antioxidant activity and their potential function as substrate for enzymatic browning reactions, the new compounds are highly characteristic for both the inner bark of Q. saponaria and commercial extracts derived therefrom.
26375986	5	14	theme	new	1013:1015	arg1	characteristic					1038:1051	characteristic	1038:1051	characteristic	1038:1051	Besides their antioxidant activity and their potential function as substrate for enzymatic browning reactions, the new compounds are highly characteristic for both the inner bark of Q. saponaria and commercial extracts derived therefrom.
26375986	1	15	theme	aqueous	153:159	arg1	extracts					161:168	freshly prepared aqueous extracts	136:168	freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina	136:215	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	0	16	theme	Quillaja	81:88	arg1	saponaria					90:98	Quillaja saponaria	81:98	Quillaja saponaria Molina	81:105	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	2	17	dep	-piscidic	388:396	arg1	+					386:386	+	386:386	+	386:386	Major phenolics in both extracts were (+)-piscidic acid and several p-coumaroyl sucrose esters.
26375986	5	18	theme	commercial	1097:1106	arg1	extracts					1108:1115	commercial extracts	1097:1115	commercial extracts derived therefrom	1097:1133	Besides their antioxidant activity and their potential function as substrate for enzymatic browning reactions, the new compounds are highly characteristic for both the inner bark of Q. saponaria and commercial extracts derived therefrom.
26375986	1	19	theme	extracts	161:168	arg1	composition					121:131	The phenolic composition	108:131	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina	108:215	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	2	20	theme	several	407:413	arg1	esters					435:440	(+)-piscidic acid and several p-coumaroyl sucrose esters	385:440	esters	435:440	Major phenolics in both extracts were (+)-piscidic acid and several p-coumaroyl sucrose esters.
26375986	3	21	theme	quillajaside	611:622	arg1	A					624:624	(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-	579:675	A	624:624	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	22	theme	1→4	522:524	arg1	-β-d-fruf					734:742	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf	512:742	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	512:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	22	theme	1→4	522:524	arg1	B					758:758	quillajaside B	745:758	quillajaside B	745:758	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	6	23	theme	authenticity	1170:1181	arg1	markers					1183:1189	authenticity markers	1170:1189	authenticity markers for the detection of Quillaja extracts in food and cosmetic formulations	1170:1262	Consequently, they might serve as authenticity markers for the detection of Quillaja extracts in food and cosmetic formulations.
26375986	6	23	theme	authenticity	1170:1181	arg1	they					1150:1153	they	1150:1153	they	1150:1153	Consequently, they might serve as authenticity markers for the detection of Quillaja extracts in food and cosmetic formulations.
26375986	1	24	theme	available	254:262	arg1	extracts					273:280	commercially available Quillaja extracts	241:280	commercially available Quillaja extracts	241:280	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	1	24	theme	available	254:262	arg1	emulsifiers					311:321	emulsifiers	311:321	emulsifiers in foods and cosmetics	311:344	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	0	25	theme	Phenylpropanoid	27:41	arg1	Esters					51:56	New Phenylpropanoid Sucrose Esters	23:56	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.	0:106	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	1	26	theme	Quillaja	264:271	arg1	extracts					273:280	commercially available Quillaja extracts	241:280	commercially available Quillaja extracts	241:280	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	1	26	theme	Quillaja	264:271	arg1	emulsifiers					311:321	emulsifiers	311:321	emulsifiers in foods and cosmetics	311:344	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	3	27	theme	α-l-rhap-	512:520	arg1	-β-d-fruf					734:742	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf	512:742	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	512:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	27	theme	α-l-rhap-	512:520	arg1	B					758:758	quillajaside B	745:758	quillajaside B	745:758	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	4	28	theme	related	835:841	arg1	esters					863:868	at least 20 structurally related p-coumaroyl sucrose esters	810:868	at least 20 structurally related p-coumaroyl sucrose esters	810:868	In addition, a putative biosynthetic pathway of at least 20 structurally related p-coumaroyl sucrose esters was tentatively identified.
26375986	1	29	theme	inner	177:181	arg1	bark					183:186	the inner bark	173:186	the inner bark of Quillaja saponaria Molina	173:215	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	2	30	theme	-piscidic	388:396	arg1	acid					398:401	(+)-piscidic acid and several p-coumaroyl sucrose esters	385:440	acid	398:401	Major phenolics in both extracts were (+)-piscidic acid and several p-coumaroyl sucrose esters.
26375986	3	31	theme	-β-d-fruf	600:608	arg1	A					624:624	(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-	579:675	A	624:624	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	0	32	theme	Molina	100:105	arg1	Bark					73:76	the Inner Bark	63:76	the Inner Bark of Quillaja saponaria Molina	63:105	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	3	33	theme	-p-coumaroyl	550:561	arg1	-α-d-glup-					563:572	4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)	543:577	4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)	543:577	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	34	theme	new	465:467	arg1	characterized					497:509	characterized	497:509	characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	497:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	34	theme	new	465:467	arg1	compounds					469:477	two new compounds	461:477	two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	461:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	4	35	theme	esters	863:868	arg1	pathway					799:805	a putative biosynthetic pathway	775:805	a putative biosynthetic pathway of at least 20 structurally related p-coumaroyl sucrose esters	775:868	In addition, a putative biosynthetic pathway of at least 20 structurally related p-coumaroyl sucrose esters was tentatively identified.
26375986	1	36	theme	bark	183:186	arg1	extracts					161:168	freshly prepared aqueous extracts	136:168	freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina	136:215	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	0	37	theme	New	23:25	arg1	Esters					51:56	New Phenylpropanoid Sucrose Esters	23:56	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.	0:106	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	5	38	theme	inner	1066:1070	arg1	bark					1072:1075	the inner bark	1062:1075	the inner bark of Q. saponaria	1062:1091	Besides their antioxidant activity and their potential function as substrate for enzymatic browning reactions, the new compounds are highly characteristic for both the inner bark of Q. saponaria and commercial extracts derived therefrom.
26375986	5	39	theme	browning	989:996	arg1	reactions					998:1006	enzymatic browning reactions	979:1006	enzymatic browning reactions	979:1006	Besides their antioxidant activity and their potential function as substrate for enzymatic browning reactions, the new compounds are highly characteristic for both the inner bark of Q. saponaria and commercial extracts derived therefrom.
26375986	6	40	theme	Quillaja	1212:1219	arg1	extracts					1221:1228	Quillaja extracts	1212:1228	Quillaja extracts	1212:1228	Consequently, they might serve as authenticity markers for the detection of Quillaja extracts in food and cosmetic formulations.
26375986	4	41	theme	sucrose	855:861	arg1	esters					863:868	at least 20 structurally related p-coumaroyl sucrose esters	810:868	at least 20 structurally related p-coumaroyl sucrose esters	810:868	In addition, a putative biosynthetic pathway of at least 20 structurally related p-coumaroyl sucrose esters was tentatively identified.
26375986	3	42	theme	β-d-apif-	631:639	arg1	-α-l-rhap-					660:669	(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-	579:675	-α-l-rhap-	660:669	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	43	theme	E	548:548	arg1	-α-d-glup-					563:572	4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)	543:577	4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)	543:577	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	1	44	theme	Quillaja	191:198	arg1	saponaria					200:208	Quillaja saponaria	191:208	Quillaja saponaria Molina	191:215	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	0	45	theme	Sucrose	43:49	arg1	Esters					51:56	New Phenylpropanoid Sucrose Esters	23:56	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.	0:106	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	3	46	theme	A	624:624	arg1	-α-d-glup-					697:706	(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-	542:712	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	512:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	46	theme	A	624:624	arg1	-p-coumaroyl					684:695	4-O-(E)-p-coumaroyl	677:695	4-O-(E)-p-coumaroyl	677:695	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	46	theme	A	624:624	arg1	-p-coumaroyl					721:732	3-O-(E)-p-coumaroyl	714:732	3-O-(E)-p-coumaroyl	714:732	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	47	theme	-α-l-rhap-	526:535	arg1	-β-d-fruf					734:742	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf	512:742	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	512:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	47	theme	-α-l-rhap-	526:535	arg1	B					758:758	quillajaside B	745:758	quillajaside B	745:758	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	2	48	from	phenolics	353:361	arg1	extracts					371:378	both extracts	366:378	both extracts	366:378	Major phenolics in both extracts were (+)-piscidic acid and several p-coumaroyl sucrose esters.
26375986	3	49	theme	latter	453:458	arg1	characterized					497:509	characterized	497:509	characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	497:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	49	theme	latter	453:458	arg1	compounds					469:477	two new compounds	461:477	two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	461:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	1	50	from	emulsifiers	311:321	arg1	cosmetics					336:344	cosmetics	336:344	cosmetics	336:344	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	1	50	from	emulsifiers	311:321	arg1	foods					326:330	foods	326:330	foods	326:330	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	5	51	theme	antioxidant	912:922	arg1	activity					924:931	their antioxidant activity	906:931	their antioxidant activity	906:931	Besides their antioxidant activity and their potential function as substrate for enzymatic browning reactions, the new compounds are highly characteristic for both the inner bark of Q. saponaria and commercial extracts derived therefrom.
26375986	3	52	theme	3-O-	580:583	arg1	-p-coumaroyl					587:598	3-O-(E)-p-coumaroyl	580:598	3-O-(E)-p-coumaroyl	580:598	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	52	theme	3-O-	580:583	arg1	E					585:585	E	585:585	E	585:585	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	2	53	theme	p-coumaroyl	415:425	arg1	esters					435:440	(+)-piscidic acid and several p-coumaroyl sucrose esters	385:440	esters	435:440	Major phenolics in both extracts were (+)-piscidic acid and several p-coumaroyl sucrose esters.
26375986	5	54	theme	Q.	1080:1081	arg1	saponaria					1083:1091	Q. saponaria	1080:1091	Q. saponaria	1080:1091	Besides their antioxidant activity and their potential function as substrate for enzymatic browning reactions, the new compounds are highly characteristic for both the inner bark of Q. saponaria and commercial extracts derived therefrom.
26375986	3	55	theme	3-O-	714:717	arg1	E					719:719	E	719:719	E	719:719	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	55	theme	3-O-	714:717	arg1	-α-d-glup-					697:706	(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-	542:712	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	512:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	55	theme	3-O-	714:717	arg1	-p-coumaroyl					721:732	3-O-(E)-p-coumaroyl	714:732	3-O-(E)-p-coumaroyl	714:732	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	4	56	theme	p-coumaroyl	843:853	arg1	esters					863:868	at least 20 structurally related p-coumaroyl sucrose esters	810:868	at least 20 structurally related p-coumaroyl sucrose esters	810:868	In addition, a putative biosynthetic pathway of at least 20 structurally related p-coumaroyl sucrose esters was tentatively identified.
26375986	5	57	theme	saponaria	1083:1091	arg1	bark					1072:1075	the inner bark	1062:1075	the inner bark of Q. saponaria	1062:1091	Besides their antioxidant activity and their potential function as substrate for enzymatic browning reactions, the new compounds are highly characteristic for both the inner bark of Q. saponaria and commercial extracts derived therefrom.
26375986	5	57	theme	saponaria	1083:1091	arg1	extracts					1108:1115	commercial extracts	1097:1115	commercial extracts derived therefrom	1097:1133	Besides their antioxidant activity and their potential function as substrate for enzymatic browning reactions, the new compounds are highly characteristic for both the inner bark of Q. saponaria and commercial extracts derived therefrom.
26375986	3	58	theme	4-O-	543:546	arg1	-α-d-glup-					563:572	4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)	543:577	4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)	543:577	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	6	59	dep	food	1233:1236	arg1	formulations					1251:1262	formulations	1251:1262	formulations	1251:1262	Consequently, they might serve as authenticity markers for the detection of Quillaja extracts in food and cosmetic formulations.
26375986	1	60	theme	saponaria	200:208	arg1	Molina					210:215	Quillaja saponaria Molina	191:215	Quillaja saponaria Molina	191:215	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	3	61	theme	-α-d-glup-	563:572	arg1	-α-d-glup-					697:706	(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-	542:712	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	512:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	61	theme	-α-d-glup-	563:572	arg1	-p-coumaroyl					684:695	4-O-(E)-p-coumaroyl	677:695	4-O-(E)-p-coumaroyl	677:695	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	61	theme	-α-d-glup-	563:572	arg1	-p-coumaroyl					721:732	3-O-(E)-p-coumaroyl	714:732	3-O-(E)-p-coumaroyl	714:732	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	1	62	theme	phenolic	112:119	arg1	composition					121:131	The phenolic composition	108:131	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina	108:215	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	1	63	theme	Molina	210:215	arg1	bark					183:186	the inner bark	173:186	the inner bark of Quillaja saponaria Molina	173:215	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	0	64	from	Bark	73:76	arg1	Esters					51:56	New Phenylpropanoid Sucrose Esters	23:56	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.	0:106	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	4	65	theme	biosynthetic	786:797	arg1	pathway					799:805	a putative biosynthetic pathway	775:805	a putative biosynthetic pathway of at least 20 structurally related p-coumaroyl sucrose esters	775:868	In addition, a putative biosynthetic pathway of at least 20 structurally related p-coumaroyl sucrose esters was tentatively identified.
26375986	2	66	theme	Major	347:351	arg1	phenolics					353:361	Major phenolics	347:361	Major phenolics in both extracts	347:378	Major phenolics in both extracts were (+)-piscidic acid and several p-coumaroyl sucrose esters.
26375986	3	67	theme	1→4	641:643	arg1	-α-l-rhap-					660:669	(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-	579:675	-α-l-rhap-	660:669	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	2	68	theme	sucrose	427:433	arg1	esters					435:440	(+)-piscidic acid and several p-coumaroyl sucrose esters	385:440	esters	435:440	Major phenolics in both extracts were (+)-piscidic acid and several p-coumaroyl sucrose esters.
26375986	1	69	used	used	303:306	arg2	emulsifiers					311:321	emulsifiers	311:321	emulsifiers in foods and cosmetics	311:344	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	1	69	used	used	303:306	arg2	extracts					273:280	commercially available Quillaja extracts	241:280	commercially available Quillaja extracts	241:280	The phenolic composition of freshly prepared aqueous extracts of the inner bark of Quillaja saponaria Molina was compared to that of commercially available Quillaja extracts, which are currently used as emulsifiers in foods and cosmetics.
26375986	0	70	dep	Quillajasides	0:12	arg1	Esters					51:56	New Phenylpropanoid Sucrose Esters	23:56	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.	0:106	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	0	70	dep	Quillajasides	0:12	arg1	B					20:20	B	20:20	B	20:20	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	0	70	dep	Quillajasides	0:12	arg1	A					14:14	A	14:14	A	14:14	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	0	70	dep	Quillajasides	0:12	arg1	Quillajasides					0:12	Quillajasides A and B	0:20	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.	0:106	Quillajasides A and B: New Phenylpropanoid Sucrose Esters from the Inner Bark of Quillaja saponaria Molina.
26375986	3	71	theme	-α-l-rhap-	645:654	arg1	-α-l-rhap-					660:669	(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-	579:675	-α-l-rhap-	660:669	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	72	theme	E	682:682	arg1	-α-d-glup-					697:706	(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-	542:712	α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B)	512:759	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
26375986	3	72	theme	E	682:682	arg1	-p-coumaroyl					684:695	4-O-(E)-p-coumaroyl	677:695	4-O-(E)-p-coumaroyl	677:695	Among the latter, two new compounds were isolated and characterized: α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside A) and β-d-apif-(1→4)-α-l-rhap-(1→4)-α-l-rhap-(1→3)-(4-O-(E)-p-coumaroyl)-α-d-glup-(1→2)-(3-O-(E)-p-coumaroyl)-β-d-fruf (quillajaside B).
27722373	6	0	theme	galactan	1187:1194	arg1	amounts					1171:1177	higher amounts	1164:1177	higher amounts of RG-I galactan that interact strongly with cellulose	1164:1232	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
27722373	6	0	theme	galactan	1187:1194	arg1	galactan					1187:1194	RG-I galactan	1182:1194	RG-I galactan	1182:1194	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
27722373	1	1	dep	in	243:244	arg1	vitro					246:250	vitro	246:250	vitro	246:250	The digestibility of starchy foods, such as potatoes, can be characterized by the proportion of starch that is rapidly digestible by in vitro hydrolysis (rapidly digestible starch, RDS).
27722373	3	2	theme	isolated	628:635	arg1	starch					637:642	their isolated starch	622:642	their isolated starch	622:642	In Crop39, Crop71 and Crop85, the properties of their isolated starch did not differ significantly from that of five control lines with higher RDS contents.
27722373	4	3	theme	other	779:783	arg1	lines					785:789	other lines	779:789	other lines tested	779:796	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	4	4	theme	side-chains	900:910	arg1	side-chains					900:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	4	4	theme	side-chains	900:910	arg1	amount					853:858	the amount	849:858	at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction	829:990	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	3	5	theme	higher	710:715	arg1	contents					721:728	higher RDS contents	710:728	higher RDS contents	710:728	In Crop39, Crop71 and Crop85, the properties of their isolated starch did not differ significantly from that of five control lines with higher RDS contents.
27722373	2	6	theme	advanced	415:422	arg1	Crop71					439:444	Crop71	439:444	Crop71	439:444	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	2	6	theme	advanced	415:422	arg1	Crop39					431:436	Crop39	431:436	Crop39	431:436	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	2	6	theme	advanced	415:422	arg1	lines					424:428	three advanced lines	409:428	three advanced lines	409:428	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	2	6	theme	advanced	415:422	arg1	Crop85					450:455	Crop85	450:455	Crop85	450:455	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	4	7	attach	attached	934:941	arg2	side-chains					900:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	4	7	attach	attached	934:941	arg1	wall					950:953	the wall	946:953	the wall	946:953	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	4	7	attach	attached	934:941	arg2	amount					853:858	the amount	849:858	at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction	829:990	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	1	8	theme	starchy	131:137	arg1	foods					139:143	starchy foods	131:143	starchy foods	131:143	The digestibility of starchy foods, such as potatoes, can be characterized by the proportion of starch that is rapidly digestible by in vitro hydrolysis (rapidly digestible starch, RDS).
27722373	1	9	theme	starch	206:211	arg1	proportion					192:201	the proportion	188:201	the proportion of starch that is rapidly digestible by in vitro hydrolysis (rapidly digestible starch, RDS)	188:294	The digestibility of starchy foods, such as potatoes, can be characterized by the proportion of starch that is rapidly digestible by in vitro hydrolysis (rapidly digestible starch, RDS).
27722373	0	10	dep	tuberosum	95:103	arg1	L					105:105	L	105:105	Solanum tuberosum L.	87:106	Composition and structure of tuber cell walls affect in vitro digestibility of potato (Solanum tuberosum L.).
27722373	5	11	theme	other	1096:1100	arg1	lines					1102:1106	other lines	1096:1106	other lines tested	1096:1113	Pectin solubilization during cooking was also remarkably low (2-4%) in these three lines compared with other lines tested (7-19%).
27722373	0	12	theme	potato	79:84	arg1	digestibility					62:74	in vitro digestibility	53:74	in vitro digestibility of potato (Solanum tuberosum L.)	53:107	Composition and structure of tuber cell walls affect in vitro digestibility of potato (Solanum tuberosum L.).
27722373	2	13	theme	P	563:563	arg1	<					565:565	P < 0.05	563:570	P < 0.05	563:570	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	2	13	theme	P	563:563	arg1	starches					553:560	their respective isolated starches	527:560	their respective isolated starches (P < 0.05)	527:571	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	6	14	theme	starch	1369:1374	arg1	hydrolysis					1376:1385	starch hydrolysis	1369:1385	starch hydrolysis	1369:1385	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
27722373	6	15	theme	higher	1164:1169	arg1	amounts					1171:1177	higher amounts	1164:1177	higher amounts of RG-I galactan that interact strongly with cellulose	1164:1232	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
27722373	6	15	theme	higher	1164:1169	arg1	galactan					1187:1194	RG-I galactan	1182:1194	RG-I galactan	1182:1194	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
27722373	5	16	from	lines	1076:1080	arg1	low					1050:1052	low	1050:1052	low	1050:1052	Pectin solubilization during cooking was also remarkably low (2-4%) in these three lines compared with other lines tested (7-19%).
27722373	4	17	theme	M	971:971	arg1	KOH					973:975	4 M KOH	969:975	4 M KOH	969:975	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	1	18	theme	foods	139:143	arg1	digestibility					114:126	The digestibility	110:126	The digestibility of starchy foods	110:143	The digestibility of starchy foods, such as potatoes, can be characterized by the proportion of starch that is rapidly digestible by in vitro hydrolysis (rapidly digestible starch, RDS).
27722373	2	19	theme	isolated	544:551	arg1	<					565:565	P < 0.05	563:570	P < 0.05	563:570	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	2	19	theme	isolated	544:551	arg1	starches					553:560	their respective isolated starches	527:560	their respective isolated starches (P < 0.05)	527:571	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	0	20	dep	potato	79:84	arg1	tuberosum					95:103	Solanum tuberosum	87:103	Solanum tuberosum L.	87:106	Composition and structure of tuber cell walls affect in vitro digestibility of potato (Solanum tuberosum L.).
27722373	6	21	theme	enzymes	1357:1363	arg1	access					1337:1342	access	1337:1342	access of digestive enzymes for starch hydrolysis	1337:1385	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
27722373	2	22	theme	RDS	322:324	arg1	content					326:332	the RDS content	318:332	the RDS content in a potato germplasm collection consisting of 98 genotypes	318:392	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	4	23	theme	RG-I	885:888	arg1	side-chains					900:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	1	24	theme	in	243:244	arg1	hydrolysis					252:261	in vitro hydrolysis	243:261	in vitro hydrolysis (rapidly digestible starch, RDS)	243:294	The digestibility of starchy foods, such as potatoes, can be characterized by the proportion of starch that is rapidly digestible by in vitro hydrolysis (rapidly digestible starch, RDS).
27722373	1	24	theme	in	243:244	arg1	starch					283:288	rapidly digestible starch	264:288	rapidly digestible starch	264:288	The digestibility of starchy foods, such as potatoes, can be characterized by the proportion of starch that is rapidly digestible by in vitro hydrolysis (rapidly digestible starch, RDS).
27722373	2	25	theme	respective	533:542	arg1	<					565:565	P < 0.05	563:570	P < 0.05	563:570	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	2	25	theme	respective	533:542	arg1	starches					553:560	their respective isolated starches	527:560	their respective isolated starches (P < 0.05)	527:571	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	6	26	theme	digestive	1347:1355	arg1	enzymes					1357:1363	digestive enzymes	1347:1363	digestive enzymes	1347:1363	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
27722373	6	27	theme	sturdier	1248:1255	arg1	wall					1257:1260	a sturdier wall	1246:1260	a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model	1246:1406	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
27722373	3	28	theme	starch	637:642	arg1	properties					608:617	the properties	604:617	the properties of their isolated starch	604:642	In Crop39, Crop71 and Crop85, the properties of their isolated starch did not differ significantly from that of five control lines with higher RDS contents.
27722373	4	29	theme	Cell	731:734	arg1	analyses					741:748	Cell wall analyses	731:748	Cell wall analyses	731:748	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	4	30	theme	galactan	891:898	arg1	side-chains					900:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	2	31	theme	potato	471:476	arg1	content					482:488	cooked potato RDS content	464:488	cooked potato RDS content	464:488	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	6	32	theme	RG-I	1182:1185	arg1	galactan					1187:1194	RG-I galactan	1182:1194	RG-I galactan	1182:1194	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
27722373	2	33	theme	germplasm	346:354	arg1	collection					356:365	a potato germplasm collection	337:365	a potato germplasm collection consisting of 98 genotypes	337:392	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	4	34	theme	wall	736:739	arg1	analyses					741:748	Cell wall analyses	731:748	Cell wall analyses	731:748	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	2	35	theme	cooked	464:469	arg1	content					482:488	cooked potato RDS content	464:488	cooked potato RDS content	464:488	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	0	36	dep	in	53:54	arg1	vitro					56:60	vitro	56:60	vitro	56:60	Composition and structure of tuber cell walls affect in vitro digestibility of potato (Solanum tuberosum L.).
27722373	4	37	theme	4	969:969	arg1	M					971:971	M	971:971	M	971:971	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	2	38	theme	potato	339:344	arg1	collection					356:365	a potato germplasm collection	337:365	a potato germplasm collection consisting of 98 genotypes	337:392	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	0	39	theme	cell	35:38	arg1	walls					40:44	tuber cell walls	29:44	tuber cell walls	29:44	Composition and structure of tuber cell walls affect in vitro digestibility of potato (Solanum tuberosum L.).
27722373	1	40	dep	starch	283:288	arg1	RDS					291:293	RDS	291:293	RDS	291:293	The digestibility of starchy foods, such as potatoes, can be characterized by the proportion of starch that is rapidly digestible by in vitro hydrolysis (rapidly digestible starch, RDS).
27722373	5	41	from	low	1050:1052	arg1	lines					1076:1080	these three lines	1064:1080	these three lines	1064:1080	Pectin solubilization during cooking was also remarkably low (2-4%) in these three lines compared with other lines tested (7-19%).
27722373	1	42	dep	such	146:149	arg1	as					151:152	as	151:152	as	151:152	The digestibility of starchy foods, such as potatoes, can be characterized by the proportion of starch that is rapidly digestible by in vitro hydrolysis (rapidly digestible starch, RDS).
27722373	0	43	theme	tuber	29:33	arg1	walls					40:44	tuber cell walls	29:44	tuber cell walls	29:44	Composition and structure of tuber cell walls affect in vitro digestibility of potato (Solanum tuberosum L.).
27722373	3	44	theme	RDS	717:719	arg1	contents					721:728	higher RDS contents	710:728	higher RDS contents	710:728	In Crop39, Crop71 and Crop85, the properties of their isolated starch did not differ significantly from that of five control lines with higher RDS contents.
27722373	3	45	with	lines	699:703	arg1	contents					721:728	higher RDS contents	710:728	higher RDS contents	710:728	In Crop39, Crop71 and Crop85, the properties of their isolated starch did not differ significantly from that of five control lines with higher RDS contents.
27722373	4	46	theme	rhamnogalacturonan-I	863:882	arg1	side-chains					900:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	5	47	theme	Pectin	993:998	arg1	solubilization					1000:1013	Pectin solubilization	993:1013	Pectin solubilization during cooking	993:1028	Pectin solubilization during cooking was also remarkably low (2-4%) in these three lines compared with other lines tested (7-19%).
27722373	4	48	dep	times	843:847	arg1	side-chains					900:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	rhamnogalacturonan-I (RG-I) galactan side-chains	863:910	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	4	48	dep	times	843:847	arg1	amount					853:858	the amount	849:858	at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction	829:990	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	4	49	contain	had	825:827	arg1	Crop85					818:823	Crop85	818:823	Crop85	818:823	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	4	49	contain	had	825:827	arg2	times					843:847	at least four times	829:847	at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction	829:990	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	4	49	contain	had	825:827	arg1	Crop71					807:812	Crop71	807:812	Crop71	807:812	Cell wall analyses revealed that, compared with other lines tested, Crop39, Crop71 and Crop85 had at least four times the amount of rhamnogalacturonan-I (RG-I) galactan side-chains that were very firmly attached to the wall and requiring 4 M KOH for extraction.
27722373	0	50	theme	walls	40:44	arg1	structure					16:24	structure	16:24	structure	16:24	Composition and structure of tuber cell walls affect in vitro digestibility of potato (Solanum tuberosum L.).
27722373	0	50	theme	walls	40:44	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and structure of tuber cell walls affect in vitro digestibility of potato (Solanum tuberosum L.).
27722373	2	51	from	content	326:332	arg1	collection					356:365	a potato germplasm collection	337:365	a potato germplasm collection consisting of 98 genotypes	337:392	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	2	52	theme	RDS	478:480	arg1	content					482:488	cooked potato RDS content	464:488	cooked potato RDS content	464:488	This study evaluated the RDS content in a potato germplasm collection consisting of 98 genotypes and identified three advanced lines, Crop39, Crop71 and Crop85, where cooked potato RDS content was significantly lower than that of their respective isolated starches (P < 0.05).
27722373	5	53	dep	low	1050:1052	arg1	%					1120:1120	7-19%	1116:1120	7-19%	1116:1120	Pectin solubilization during cooking was also remarkably low (2-4%) in these three lines compared with other lines tested (7-19%).
27722373	5	53	dep	low	1050:1052	arg1	%					1058:1058	2-4%	1055:1058	2-4%	1055:1058	Pectin solubilization during cooking was also remarkably low (2-4%) in these three lines compared with other lines tested (7-19%).
27722373	6	54	theme	in	1393:1394	arg1	model					1402:1406	an in vitro model	1390:1406	an in vitro model	1390:1406	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
27722373	6	55	dep	in	1393:1394	arg1	vitro					1396:1400	vitro	1396:1400	vitro	1396:1400	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
27722373	3	56	theme	control	691:697	arg1	lines					699:703	five control lines	686:703	five control lines with higher RDS contents	686:728	In Crop39, Crop71 and Crop85, the properties of their isolated starch did not differ significantly from that of five control lines with higher RDS contents.
27722373	0	57	theme	in	53:54	arg1	digestibility					62:74	in vitro digestibility	53:74	in vitro digestibility of potato (Solanum tuberosum L.)	53:107	Composition and structure of tuber cell walls affect in vitro digestibility of potato (Solanum tuberosum L.).
27722373	1	58	theme	digestible	272:281	arg1	hydrolysis					252:261	in vitro hydrolysis	243:261	in vitro hydrolysis (rapidly digestible starch, RDS)	243:294	The digestibility of starchy foods, such as potatoes, can be characterized by the proportion of starch that is rapidly digestible by in vitro hydrolysis (rapidly digestible starch, RDS).
27722373	1	58	theme	digestible	272:281	arg1	starch					283:288	rapidly digestible starch	264:288	rapidly digestible starch	264:288	The digestibility of starchy foods, such as potatoes, can be characterized by the proportion of starch that is rapidly digestible by in vitro hydrolysis (rapidly digestible starch, RDS).
27722373	6	59	theme	amounts	1171:1177	arg1	possession					1150:1159	possession	1150:1159	possession of higher amounts of RG-I galactan that interact strongly with cellulose	1150:1232	The findings suggest that possession of higher amounts of RG-I galactan that interact strongly with cellulose may provide a sturdier wall that better resists solubilization during cooking, and effectively impedes access of digestive enzymes for starch hydrolysis in an in vitro model.
26118486	2	0	theme	present	113:119	arg1	study					121:125	the present study	109:125	the present study	109:125	The objective of the present study is to characterize the physicochemical properties and to determine the in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	4	1	theme	uronic	324:329	arg1	acid					331:334	uronic acid and protein contents	324:355	acid	331:334	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	11	2	attach	present	1279:1285	arg2	Arabinose					1213:1221	Arabinose	1213:1221	Arabinose	1213:1221	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	11	2	attach	present	1279:1285	arg2	monosaccharides					1263:1277	the main monosaccharides	1254:1277	the main monosaccharides present in the gum exudates	1254:1305	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	11	2	attach	present	1279:1285	arg2	galactose					1227:1235	galactose	1227:1235	galactose	1227:1235	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	11	2	attach	present	1279:1285	arg1	exudates					1298:1305	the gum exudates	1290:1305	the gum exudates	1290:1305	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	12	3	theme	hydroxyl	1411:1418	arg1	scavenging					1420:1429	hydroxyl scavenging	1411:1429	hydroxyl scavenging	1411:1429	Results from DPPH, hydroxyl scavenging and reducing power studies showed the gum possesses antioxidant properties.
26118486	12	4	theme	reducing	1435:1442	arg1	studies					1450:1456	reducing power studies	1435:1456	reducing power studies	1435:1456	Results from DPPH, hydroxyl scavenging and reducing power studies showed the gum possesses antioxidant properties.
26118486	8	5	theme	antioxidant	831:841	arg1	properties					843:852	The antioxidant properties	827:852	The antioxidant properties of the gum	827:863	The antioxidant properties of the gum were evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl scavenging activities and reducing power.
26118486	11	6	theme	gum	1294:1296	arg1	exudates					1298:1305	the gum exudates	1290:1305	the gum exudates	1290:1305	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	4	7	theme	weight	403:408	arg1	distribution					410:421	the molecular weight distribution	389:421	the molecular weight distribution	389:421	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	7	8	theme	TGA/DTA	751:757	arg1	analyses					760:767	thermogravimetry (TGA/DTA) analyses	733:767	thermogravimetry (TGA/DTA) analyses	733:767	Differential scanning calorimetry (DSC) and thermogravimetry (TGA/DTA) analyses were performed to study the thermal stability of the gum.
26118486	4	9	theme	molecular	393:401	arg1	distribution					410:421	the molecular weight distribution	389:421	the molecular weight distribution	389:421	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	5	10	theme	gum	549:551	arg1	exudates					553:560	the dried gum exudates	539:560	the dried gum exudates	539:560	The powder flow property and preliminary compressibility test were performed on the dried gum exudates.
26118486	12	11	from	scavenging	1420:1429	arg1	Results					1392:1398	Results	1392:1398	Results from DPPH, hydroxyl scavenging and reducing power studies	1392:1456	Results from DPPH, hydroxyl scavenging and reducing power studies showed the gum possesses antioxidant properties.
26118486	10	12	theme	%	1115:1115	arg1	solution					1125:1132	2% aqueous solution	1114:1132	2% aqueous solution of the gum	1114:1143	The viscosity of 2% aqueous solution of the gum exhibited non-Newtonian type of flow showing pH dependent swelling.
26118486	11	13	theme	present	1279:1285	arg1	monosaccharides					1263:1277	the main monosaccharides	1254:1277	the main monosaccharides present in the gum exudates	1254:1305	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	11	13	theme	present	1279:1285	arg1	galactose					1227:1235	galactose	1227:1235	galactose	1227:1235	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	11	13	theme	present	1279:1285	arg1	Arabinose					1213:1221	Arabinose	1213:1221	Arabinose	1213:1221	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	12	14	theme	power	1444:1448	arg1	studies					1450:1456	reducing power studies	1435:1456	reducing power studies	1435:1456	Results from DPPH, hydroxyl scavenging and reducing power studies showed the gum possesses antioxidant properties.
26118486	5	15	theme	compressibility	500:514	arg1	test					516:519	preliminary compressibility test	488:519	preliminary compressibility test	488:519	The powder flow property and preliminary compressibility test were performed on the dried gum exudates.
26118486	7	16	theme	thermogravimetry	733:748	arg1	analyses					760:767	thermogravimetry (TGA/DTA) analyses	733:767	thermogravimetry (TGA/DTA) analyses	733:767	Differential scanning calorimetry (DSC) and thermogravimetry (TGA/DTA) analyses were performed to study the thermal stability of the gum.
26118486	12	17	from	studies	1450:1456	arg1	Results					1392:1398	Results	1392:1398	Results from DPPH, hydroxyl scavenging and reducing power studies	1392:1456	Results from DPPH, hydroxyl scavenging and reducing power studies showed the gum possesses antioxidant properties.
26118486	12	18	from	DPPH	1405:1408	arg1	Results					1392:1398	Results	1392:1398	Results from DPPH, hydroxyl scavenging and reducing power studies	1392:1456	Results from DPPH, hydroxyl scavenging and reducing power studies showed the gum possesses antioxidant properties.
26118486	11	19	theme	gum	1352:1354	arg1	distribution					1332:1343	the molecular weight distribution	1311:1343	the molecular weight distribution of the gum	1311:1354	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	11	19	theme	gum	1352:1354	arg1	polydispersed					1377:1389	polydispersed	1377:1389	polydispersed	1377:1389	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	10	20	theme	dependent	1193:1201	arg1	swelling					1203:1210	pH dependent swelling	1190:1210	pH dependent swelling	1190:1210	The viscosity of 2% aqueous solution of the gum exhibited non-Newtonian type of flow showing pH dependent swelling.
26118486	3	21	theme	gum	255:257	arg1	exudates					259:266	gum exudates	255:266	gum exudates	255:266	gum exudates collected from Northeast India.
26118486	4	22	theme	total	304:308	arg1	carbohydrate					310:321	The total carbohydrate	300:321	The total carbohydrate	300:321	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	9	23	theme	protein	1011:1017	arg1	contents					1019:1026	The total carbohydrate and protein contents	984:1026	contents	1019:1026	The total carbohydrate and protein contents of the gum were found to be 75.17±3.21% and 2.60±1.05% respectively.
26118486	9	24	theme	total	988:992	arg1	%					1066:1066	75.17±3.21%	1056:1066	75.17±3.21%	1056:1066	The total carbohydrate and protein contents of the gum were found to be 75.17±3.21% and 2.60±1.05% respectively.
26118486	9	24	theme	total	988:992	arg1	carbohydrate					994:1005	The total carbohydrate and protein contents	984:1026	carbohydrate	994:1005	The total carbohydrate and protein contents of the gum were found to be 75.17±3.21% and 2.60±1.05% respectively.
26118486	6	25	dep	transform	571:579	arg1	infrared					581:588	infrared	581:588	transform infrared spectroscopy (FTIR) study	571:614	Fourier transform infrared spectroscopy (FTIR) study was performed to analyze the functional groups present in the structure.
26118486	4	26	theme	monosaccharide	358:371	arg1	composition					373:383	monosaccharide composition	358:383	monosaccharide composition	358:383	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	6	27	dep	Fourier	563:569	arg1	transform					571:579	transform	571:579	transform infrared spectroscopy (FTIR) study	571:614	Fourier transform infrared spectroscopy (FTIR) study was performed to analyze the functional groups present in the structure.
26118486	6	28	from	structure	678:686	arg1	present					663:669	present	663:669	present	663:669	Fourier transform infrared spectroscopy (FTIR) study was performed to analyze the functional groups present in the structure.
26118486	5	29	theme	flow	470:473	arg1	property					475:482	The powder flow property	459:482	The powder flow property	459:482	The powder flow property and preliminary compressibility test were performed on the dried gum exudates.
26118486	11	30	theme	molecular	1315:1323	arg1	distribution					1332:1343	the molecular weight distribution	1311:1343	the molecular weight distribution of the gum	1311:1354	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	11	30	theme	molecular	1315:1323	arg1	polydispersed					1377:1389	polydispersed	1377:1389	polydispersed	1377:1389	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	7	31	theme	gum	822:824	arg1	stability					805:813	the thermal stability	793:813	the thermal stability of the gum	793:824	Differential scanning calorimetry (DSC) and thermogravimetry (TGA/DTA) analyses were performed to study the thermal stability of the gum.
26118486	10	32	theme	non-Newtonian	1155:1167	arg1	type					1169:1172	non-Newtonian type	1155:1172	non-Newtonian type of flow showing pH dependent swelling	1155:1210	The viscosity of 2% aqueous solution of the gum exhibited non-Newtonian type of flow showing pH dependent swelling.
26118486	6	33	from	present	663:669	arg1	structure					678:686	the structure	674:686	the structure	674:686	Fourier transform infrared spectroscopy (FTIR) study was performed to analyze the functional groups present in the structure.
26118486	0	34	theme	in	21:22	arg1	activity					42:49	in vitro antioxidant activity	21:49	in vitro antioxidant activity	21:49	Characterization and in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	10	35	theme	solution	1125:1132	arg1	viscosity					1101:1109	The viscosity	1097:1109	The viscosity of 2% aqueous solution of the gum	1097:1143	The viscosity of 2% aqueous solution of the gum exhibited non-Newtonian type of flow showing pH dependent swelling.
26118486	3	36	theme	Northeast	283:291	arg1	India					293:297	Northeast India	283:297	Northeast India	283:297	gum exudates collected from Northeast India.
26118486	10	37	theme	aqueous	1117:1123	arg1	solution					1125:1132	2% aqueous solution	1114:1132	2% aqueous solution of the gum	1114:1143	The viscosity of 2% aqueous solution of the gum exhibited non-Newtonian type of flow showing pH dependent swelling.
26118486	8	38	theme	scavenging	942:951	arg1	activities					953:962	hydroxyl scavenging activities	933:962	hydroxyl scavenging activities	933:962	The antioxidant properties of the gum were evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl scavenging activities and reducing power.
26118486	2	39	theme	Albizia	231:237	arg1	Boiv					249:252	Albizia stipulata Boiv	231:252	Albizia stipulata Boiv	231:252	The objective of the present study is to characterize the physicochemical properties and to determine the in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	5	40	theme	preliminary	488:498	arg1	test					516:519	preliminary compressibility test	488:519	preliminary compressibility test	488:519	The powder flow property and preliminary compressibility test were performed on the dried gum exudates.
26118486	7	41	theme	Differential	689:700	arg1	DSC					724:726	DSC	724:726	DSC	724:726	Differential scanning calorimetry (DSC) and thermogravimetry (TGA/DTA) analyses were performed to study the thermal stability of the gum.
26118486	7	41	theme	Differential	689:700	arg1	calorimetry					711:721	Differential scanning calorimetry	689:721	Differential scanning calorimetry (DSC)	689:727	Differential scanning calorimetry (DSC) and thermogravimetry (TGA/DTA) analyses were performed to study the thermal stability of the gum.
26118486	0	42	theme	antioxidant	30:40	arg1	activity					42:49	in vitro antioxidant activity	21:49	in vitro antioxidant activity	21:49	Characterization and in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	10	43	theme	flow	1177:1180	arg1	type					1169:1172	non-Newtonian type	1155:1172	non-Newtonian type of flow showing pH dependent swelling	1155:1210	The viscosity of 2% aqueous solution of the gum exhibited non-Newtonian type of flow showing pH dependent swelling.
26118486	4	44	theme	protein	340:346	arg1	contents					348:355	uronic acid and protein contents	324:355	contents	348:355	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	0	45	theme	Albizia	54:60	arg1	Boiv					72:75	Albizia stipulata Boiv	54:75	Albizia stipulata Boiv	54:75	Characterization and in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	7	46	theme	thermal	797:803	arg1	stability					805:813	the thermal stability	793:813	the thermal stability of the gum	793:824	Differential scanning calorimetry (DSC) and thermogravimetry (TGA/DTA) analyses were performed to study the thermal stability of the gum.
26118486	2	47	theme	physicochemical	150:164	arg1	properties					166:175	the physicochemical properties	146:175	the physicochemical properties	146:175	The objective of the present study is to characterize the physicochemical properties and to determine the in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	8	48	theme	hydroxyl	933:940	arg1	activities					953:962	hydroxyl scavenging activities	933:962	hydroxyl scavenging activities	933:962	The antioxidant properties of the gum were evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl scavenging activities and reducing power.
26118486	11	49	from	present	1279:1285	arg1	exudates					1298:1305	the gum exudates	1290:1305	the gum exudates	1290:1305	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	9	50	theme	gum	1035:1037	arg1	%					1066:1066	75.17±3.21%	1056:1066	75.17±3.21%	1056:1066	The total carbohydrate and protein contents of the gum were found to be 75.17±3.21% and 2.60±1.05% respectively.
26118486	9	50	theme	gum	1035:1037	arg1	carbohydrate					994:1005	The total carbohydrate and protein contents	984:1026	carbohydrate	994:1005	The total carbohydrate and protein contents of the gum were found to be 75.17±3.21% and 2.60±1.05% respectively.
26118486	9	50	theme	gum	1035:1037	arg1	contents					1019:1026	The total carbohydrate and protein contents	984:1026	contents	1019:1026	The total carbohydrate and protein contents of the gum were found to be 75.17±3.21% and 2.60±1.05% respectively.
26118486	8	51	theme	gum	861:863	arg1	properties					843:852	The antioxidant properties	827:852	The antioxidant properties of the gum	827:863	The antioxidant properties of the gum were evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl scavenging activities and reducing power.
26118486	11	52	theme	main	1258:1261	arg1	monosaccharides					1263:1277	the main monosaccharides	1254:1277	the main monosaccharides present in the gum exudates	1254:1305	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	11	52	theme	main	1258:1261	arg1	galactose					1227:1235	galactose	1227:1235	galactose	1227:1235	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	11	52	theme	main	1258:1261	arg1	Arabinose					1213:1221	Arabinose	1213:1221	Arabinose	1213:1221	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	4	53	theme	purified	430:437	arg1	gum					439:441	the purified gum	426:441	the purified gum	426:441	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	2	54	theme	antioxidant	207:217	arg1	activity					219:226	the in vitro antioxidant activity	194:226	the in vitro antioxidant activity of Albizia stipulata Boiv	194:252	The objective of the present study is to characterize the physicochemical properties and to determine the in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	10	55	theme	gum	1141:1143	arg1	solution					1125:1132	2% aqueous solution	1114:1132	2% aqueous solution of the gum	1114:1143	The viscosity of 2% aqueous solution of the gum exhibited non-Newtonian type of flow showing pH dependent swelling.
26118486	2	56	theme	in	198:199	arg1	activity					219:226	the in vitro antioxidant activity	194:226	the in vitro antioxidant activity of Albizia stipulata Boiv	194:252	The objective of the present study is to characterize the physicochemical properties and to determine the in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	7	57	theme	scanning	702:709	arg1	DSC					724:726	DSC	724:726	DSC	724:726	Differential scanning calorimetry (DSC) and thermogravimetry (TGA/DTA) analyses were performed to study the thermal stability of the gum.
26118486	7	57	theme	scanning	702:709	arg1	calorimetry					711:721	Differential scanning calorimetry	689:721	Differential scanning calorimetry (DSC)	689:727	Differential scanning calorimetry (DSC) and thermogravimetry (TGA/DTA) analyses were performed to study the thermal stability of the gum.
26118486	12	58	dep	gum	1469:1471	arg1	possesses					1473:1481	possesses	1473:1481	possesses antioxidant properties	1473:1504	Results from DPPH, hydroxyl scavenging and reducing power studies showed the gum possesses antioxidant properties.
26118486	11	59	theme	weight	1325:1330	arg1	distribution					1332:1343	the molecular weight distribution	1311:1343	the molecular weight distribution of the gum	1311:1354	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	11	59	theme	weight	1325:1330	arg1	polydispersed					1377:1389	polydispersed	1377:1389	polydispersed	1377:1389	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	6	60	attach	present	663:669	arg1	structure					678:686	the structure	674:686	the structure	674:686	Fourier transform infrared spectroscopy (FTIR) study was performed to analyze the functional groups present in the structure.
26118486	6	60	attach	present	663:669	arg2	groups					656:661	the functional groups	641:661	the functional groups present in the structure	641:686	Fourier transform infrared spectroscopy (FTIR) study was performed to analyze the functional groups present in the structure.
26118486	12	61	theme	antioxidant	1483:1493	arg1	properties					1495:1504	antioxidant properties	1483:1504	antioxidant properties	1483:1504	Results from DPPH, hydroxyl scavenging and reducing power studies showed the gum possesses antioxidant properties.
26118486	0	62	dep	in	21:22	arg1	vitro					24:28	vitro	24:28	vitro	24:28	Characterization and in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	6	63	theme	present	663:669	arg1	groups					656:661	the functional groups	641:661	the functional groups present in the structure	641:686	Fourier transform infrared spectroscopy (FTIR) study was performed to analyze the functional groups present in the structure.
26118486	11	64	from	exudates	1298:1305	arg1	present					1279:1285	present	1279:1285	present	1279:1285	Arabinose and galactose were found to be the main monosaccharides present in the gum exudates and the molecular weight distribution of the gum was also found to be polydispersed.
26118486	0	65	theme	Boiv	72:75	arg1	activity					42:49	in vitro antioxidant activity	21:49	in vitro antioxidant activity	21:49	Characterization and in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	0	65	theme	Boiv	72:75	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	4	66	theme	gum	439:441	arg1	distribution					410:421	the molecular weight distribution	389:421	the molecular weight distribution	389:421	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	4	66	theme	gum	439:441	arg1	carbohydrate					310:321	The total carbohydrate	300:321	The total carbohydrate	300:321	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	4	66	theme	gum	439:441	arg1	acid					331:334	uronic acid and protein contents	324:355	acid	331:334	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	4	66	theme	gum	439:441	arg1	contents					348:355	uronic acid and protein contents	324:355	contents	348:355	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	4	66	theme	gum	439:441	arg1	composition					373:383	monosaccharide composition	358:383	monosaccharide composition	358:383	The total carbohydrate, uronic acid and protein contents, monosaccharide composition and the molecular weight distribution of the purified gum was determined.
26118486	2	67	theme	Boiv	249:252	arg1	activity					219:226	the in vitro antioxidant activity	194:226	the in vitro antioxidant activity of Albizia stipulata Boiv	194:252	The objective of the present study is to characterize the physicochemical properties and to determine the in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	8	68	theme	reducing	968:975	arg1	power					977:981	reducing power	968:981	reducing power	968:981	The antioxidant properties of the gum were evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl scavenging activities and reducing power.
26118486	5	69	theme	powder	463:468	arg1	property					475:482	The powder flow property	459:482	The powder flow property	459:482	The powder flow property and preliminary compressibility test were performed on the dried gum exudates.
26118486	6	70	theme	spectroscopy	590:601	arg1	study					610:614	spectroscopy (FTIR) study	590:614	spectroscopy (FTIR) study	590:614	Fourier transform infrared spectroscopy (FTIR) study was performed to analyze the functional groups present in the structure.
26118486	0	71	theme	stipulata	62:70	arg1	Boiv					72:75	Albizia stipulata Boiv	54:75	Albizia stipulata Boiv	54:75	Characterization and in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	2	72	theme	study	121:125	arg1	objective					96:104	The objective	92:104	The objective of the present study	92:125	The objective of the present study is to characterize the physicochemical properties and to determine the in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	2	73	theme	stipulata	239:247	arg1	Boiv					249:252	Albizia stipulata Boiv	231:252	Albizia stipulata Boiv	231:252	The objective of the present study is to characterize the physicochemical properties and to determine the in vitro antioxidant activity of Albizia stipulata Boiv.
26118486	5	74	theme	dried	543:547	arg1	exudates					553:560	the dried gum exudates	539:560	the dried gum exudates	539:560	The powder flow property and preliminary compressibility test were performed on the dried gum exudates.
26118486	6	75	theme	functional	645:654	arg1	groups					656:661	the functional groups	641:661	the functional groups present in the structure	641:686	Fourier transform infrared spectroscopy (FTIR) study was performed to analyze the functional groups present in the structure.
26118486	2	76	dep	in	198:199	arg1	vitro					201:205	vitro	201:205	vitro	201:205	The objective of the present study is to characterize the physicochemical properties and to determine the in vitro antioxidant activity of Albizia stipulata Boiv.
25828508	5	0	theme	high	869:872	arg1	sensitivity					874:884	high sensitivity	869:884	high sensitivity	869:884	Without any further purification steps, oligosaccharides were analyzed by an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed.
25828508	8	1	theme	oligosaccharide	1217:1231	arg1	compounds					1233:1241	66 oligosaccharide compounds	1214:1241	66 oligosaccharide compounds detected in 52 Epimedium herbs	1214:1272	A total of 66 oligosaccharide compounds detected in 52 Epimedium herbs were relatively quantified and statistically processed by principal component analysis (PCA).
25828508	8	2	theme	Epimedium	1258:1266	arg1	herbs					1268:1272	52 Epimedium herbs	1255:1272	52 Epimedium herbs	1255:1272	A total of 66 oligosaccharide compounds detected in 52 Epimedium herbs were relatively quantified and statistically processed by principal component analysis (PCA).
25828508	9	3	theme	different	1416:1424	arg1	species					1426:1432	different species	1416:1432	different species based on their oligosaccharide composition and content	1416:1487	The 52 Epimedium herbs could be classified into different species based on their oligosaccharide composition and content.
25828508	1	4	theme	pharmacological	168:182	arg1	activities					184:193	diverse important pharmacological activities	150:193	diverse important pharmacological activities	150:193	Oligosaccharides, which exist widely in herbs, present diverse important pharmacological activities.
25828508	2	5	theme	activity	314:321	arg1	profiling					267:275	their profiling	261:275	their profiling	261:275	However, the complexity of oligosaccharides seriously challenges their profiling, quality control, and elucidation of activity.
25828508	2	5	theme	activity	314:321	arg1	control					286:292	quality control	278:292	quality control	278:292	However, the complexity of oligosaccharides seriously challenges their profiling, quality control, and elucidation of activity.
25828508	2	5	theme	activity	314:321	arg1	elucidation					299:309	elucidation	299:309	elucidation of activity	299:321	However, the complexity of oligosaccharides seriously challenges their profiling, quality control, and elucidation of activity.
25828508	3	6	theme	Epimedium	614:622	arg1	oligosaccharides					594:609	the oligosaccharides	590:609	the oligosaccharides of Epimedium	590:622	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	6	7	theme	MS	1011:1012	arg1	orders					1018:1023	the MS two orders	1007:1023	the MS two orders of magnitude higher than that of the free oligosaccharide	1007:1081	Benefitting from the derivatization, the oligosaccharides generated a response in the MS two orders of magnitude higher than that of the free oligosaccharide.
25828508	3	8	theme	analytical	363:372	arg1	method					374:379	a novel oligosaccharide analytical method	339:379	a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure	339:542	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	10	9	theme	MS/MS	1654:1658	arg1	spectra					1660:1666	MS/MS spectra	1654:1666	MS/MS spectra	1654:1666	Thirteen oligosaccharide compounds demonstrated potential as markers for Epimedium species classification, and their structures were preliminarily identified using MS/MS spectra.
25828508	3	10	theme	high	481:484	arg1	UPLC-HRMS					523:531	UPLC-HRMS	523:531	UPLC-HRMS	523:531	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	3	10	theme	high	481:484	arg1	spectrometry					509:520	high resolution tandem mass spectrometry	481:520	high resolution tandem mass spectrometry (UPLC-HRMS) procedure	481:542	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	5	11	theme	UPLC-HRMS/MS	844:855	arg1	method					857:862	an established UPLC-HRMS/MS method	829:862	an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed	829:922	Without any further purification steps, oligosaccharides were analyzed by an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed.
25828508	3	12	theme	resolution	486:495	arg1	UPLC-HRMS					523:531	UPLC-HRMS	523:531	UPLC-HRMS	523:531	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	3	12	theme	resolution	486:495	arg1	spectrometry					509:520	high resolution tandem mass spectrometry	481:520	high resolution tandem mass spectrometry (UPLC-HRMS) procedure	481:542	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	3	13	theme	derivatization	396:409	arg1	pretreatment					411:422	a new derivatization pretreatment and ultra-performance liquid chromatography	390:466	pretreatment	411:422	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	9	14	theme	oligosaccharide	1449:1463	arg1	composition					1465:1475	their oligosaccharide composition	1443:1475	their oligosaccharide composition	1443:1475	The 52 Epimedium herbs could be classified into different species based on their oligosaccharide composition and content.
25828508	0	15	theme	oligosaccharide	68:82	arg1	profiling					84:92	UPLC-HRMS oligosaccharide profiling	58:92	UPLC-HRMS oligosaccharide profiling	58:92	Novel strategy for herbal species classification based on UPLC-HRMS oligosaccharide profiling.
25828508	0	16	theme	Novel	0:4	arg1	strategy					6:13	Novel strategy	0:13	Novel strategy for herbal species classification	0:47	Novel strategy for herbal species classification based on UPLC-HRMS oligosaccharide profiling.
25828508	10	17	theme	species	1573:1579	arg1	classification					1581:1594	Epimedium species classification	1563:1594	Epimedium species classification	1563:1594	Thirteen oligosaccharide compounds demonstrated potential as markers for Epimedium species classification, and their structures were preliminarily identified using MS/MS spectra.
25828508	9	18	theme	Epimedium	1375:1383	arg1	herbs					1385:1389	The 52 Epimedium herbs	1368:1389	The 52 Epimedium herbs	1368:1389	The 52 Epimedium herbs could be classified into different species based on their oligosaccharide composition and content.
25828508	0	19	theme	herbal	19:24	arg1	classification					34:47	herbal species classification	19:47	herbal species classification	19:47	Novel strategy for herbal species classification based on UPLC-HRMS oligosaccharide profiling.
25828508	5	20	with	method	857:862	arg1	speed					918:922	speed	918:922	speed	918:922	Without any further purification steps, oligosaccharides were analyzed by an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed.
25828508	5	20	with	method	857:862	arg1	efficiency					903:912	good separation efficiency	887:912	good separation efficiency	887:912	Without any further purification steps, oligosaccharides were analyzed by an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed.
25828508	5	20	with	method	857:862	arg1	sensitivity					874:884	high sensitivity	869:884	high sensitivity	869:884	Without any further purification steps, oligosaccharides were analyzed by an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed.
25828508	7	21	theme	spectral	1188:1195	arg1	data					1197:1200	the high-resolution mass spectral data	1163:1200	the high-resolution mass spectral data	1163:1200	Oligosaccharides of 52 Epimedium samples were profiled and identified based on the high-resolution mass spectral data.
25828508	10	22	theme	Epimedium	1563:1571	arg1	classification					1581:1594	Epimedium species classification	1563:1594	Epimedium species classification	1563:1594	Thirteen oligosaccharide compounds demonstrated potential as markers for Epimedium species classification, and their structures were preliminarily identified using MS/MS spectra.
25828508	8	23	located	detected	1243:1250	arg2	compounds					1233:1241	66 oligosaccharide compounds	1214:1241	66 oligosaccharide compounds detected in 52 Epimedium herbs	1214:1272	A total of 66 oligosaccharide compounds detected in 52 Epimedium herbs were relatively quantified and statistically processed by principal component analysis (PCA).
25828508	8	23	located	detected	1243:1250	arg1	herbs					1268:1272	52 Epimedium herbs	1255:1272	52 Epimedium herbs	1255:1272	A total of 66 oligosaccharide compounds detected in 52 Epimedium herbs were relatively quantified and statistically processed by principal component analysis (PCA).
25828508	3	24	theme	spectrometry	509:520	arg1	procedure					534:542	high resolution tandem mass spectrometry (UPLC-HRMS) procedure	481:542	high resolution tandem mass spectrometry (UPLC-HRMS) procedure	481:542	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	3	25	theme	new	392:394	arg1	pretreatment					411:422	a new derivatization pretreatment and ultra-performance liquid chromatography	390:466	pretreatment	411:422	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	6	26	theme	free	1062:1065	arg1	oligosaccharide					1067:1081	the free oligosaccharide	1058:1081	the free oligosaccharide	1058:1081	Benefitting from the derivatization, the oligosaccharides generated a response in the MS two orders of magnitude higher than that of the free oligosaccharide.
25828508	1	27	dep	diverse	150:156	arg1	important					158:166	important	158:166	important	158:166	Oligosaccharides, which exist widely in herbs, present diverse important pharmacological activities.
25828508	3	28	theme	liquid	446:451	arg1	chromatography					453:466	a new derivatization pretreatment and ultra-performance liquid chromatography	390:466	chromatography	453:466	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	7	29	theme	high-resolution	1167:1181	arg1	data					1197:1200	the high-resolution mass spectral data	1163:1200	the high-resolution mass spectral data	1163:1200	Oligosaccharides of 52 Epimedium samples were profiled and identified based on the high-resolution mass spectral data.
25828508	3	30	theme	tandem	497:502	arg1	UPLC-HRMS					523:531	UPLC-HRMS	523:531	UPLC-HRMS	523:531	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	3	30	theme	tandem	497:502	arg1	spectrometry					509:520	high resolution tandem mass spectrometry	481:520	high resolution tandem mass spectrometry (UPLC-HRMS) procedure	481:542	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	0	31	theme	species	26:32	arg1	classification					34:47	herbal species classification	19:47	herbal species classification	19:47	Novel strategy for herbal species classification based on UPLC-HRMS oligosaccharide profiling.
25828508	5	32	theme	good	887:890	arg1	efficiency					903:912	good separation efficiency	887:912	good separation efficiency	887:912	Without any further purification steps, oligosaccharides were analyzed by an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed.
25828508	8	33	theme	principal	1332:1340	arg1	analysis					1352:1359	principal component analysis	1332:1359	principal component analysis (PCA)	1332:1365	A total of 66 oligosaccharide compounds detected in 52 Epimedium herbs were relatively quantified and statistically processed by principal component analysis (PCA).
25828508	8	33	theme	principal	1332:1340	arg1	PCA					1362:1364	PCA	1362:1364	PCA	1362:1364	A total of 66 oligosaccharide compounds detected in 52 Epimedium herbs were relatively quantified and statistically processed by principal component analysis (PCA).
25828508	3	34	theme	mass	504:507	arg1	UPLC-HRMS					523:531	UPLC-HRMS	523:531	UPLC-HRMS	523:531	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	3	34	theme	mass	504:507	arg1	spectrometry					509:520	high resolution tandem mass spectrometry	481:520	high resolution tandem mass spectrometry (UPLC-HRMS) procedure	481:542	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	5	35	theme	separation	892:901	arg1	efficiency					903:912	good separation efficiency	887:912	good separation efficiency	887:912	Without any further purification steps, oligosaccharides were analyzed by an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed.
25828508	8	36	theme	component	1342:1350	arg1	analysis					1352:1359	principal component analysis	1332:1359	principal component analysis (PCA)	1332:1365	A total of 66 oligosaccharide compounds detected in 52 Epimedium herbs were relatively quantified and statistically processed by principal component analysis (PCA).
25828508	8	36	theme	component	1342:1350	arg1	PCA					1362:1364	PCA	1362:1364	PCA	1362:1364	A total of 66 oligosaccharide compounds detected in 52 Epimedium herbs were relatively quantified and statistically processed by principal component analysis (PCA).
25828508	0	37	theme	UPLC-HRMS	58:66	arg1	profiling					84:92	UPLC-HRMS oligosaccharide profiling	58:92	UPLC-HRMS oligosaccharide profiling	58:92	Novel strategy for herbal species classification based on UPLC-HRMS oligosaccharide profiling.
25828508	7	38	theme	mass	1183:1186	arg1	data					1197:1200	the high-resolution mass spectral data	1163:1200	the high-resolution mass spectral data	1163:1200	Oligosaccharides of 52 Epimedium samples were profiled and identified based on the high-resolution mass spectral data.
25828508	3	39	theme	ultra-performance	428:444	arg1	chromatography					453:466	a new derivatization pretreatment and ultra-performance liquid chromatography	390:466	chromatography	453:466	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	5	40	theme	further	767:773	arg1	steps					788:792	any further purification steps	763:792	any further purification steps	763:792	Without any further purification steps, oligosaccharides were analyzed by an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed.
25828508	2	41	theme	oligosaccharides	223:238	arg1	complexity					209:218	the complexity	205:218	the complexity of oligosaccharides	205:238	However, the complexity of oligosaccharides seriously challenges their profiling, quality control, and elucidation of activity.
25828508	7	42	theme	samples	1117:1123	arg1	Oligosaccharides					1084:1099	Oligosaccharides	1084:1099	Oligosaccharides of 52 Epimedium samples	1084:1123	Oligosaccharides of 52 Epimedium samples were profiled and identified based on the high-resolution mass spectral data.
25828508	6	43	theme	higher	1038:1043	arg1	orders					1018:1023	the MS two orders	1007:1023	the MS two orders of magnitude higher than that of the free oligosaccharide	1007:1081	Benefitting from the derivatization, the oligosaccharides generated a response in the MS two orders of magnitude higher than that of the free oligosaccharide.
25828508	5	44	theme	purification	775:786	arg1	steps					788:792	any further purification steps	763:792	any further purification steps	763:792	Without any further purification steps, oligosaccharides were analyzed by an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed.
25828508	5	45	theme	established	832:842	arg1	method					857:862	an established UPLC-HRMS/MS method	829:862	an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed	829:922	Without any further purification steps, oligosaccharides were analyzed by an established UPLC-HRMS/MS method with high sensitivity, good separation efficiency and speed.
25828508	8	46	theme	compounds	1233:1241	arg1	total					1205:1209	A total	1203:1209	A total of 66 oligosaccharide compounds detected in 52 Epimedium herbs	1203:1272	A total of 66 oligosaccharide compounds detected in 52 Epimedium herbs were relatively quantified and statistically processed by principal component analysis (PCA).
25828508	4	47	theme	2,4-bis	668:674	arg1	-6-hydrazino-1,3,5-triazine					689:715	2,4-bis(diethylamino)-6-hydrazino-1,3,5-triazine	668:715	2,4-bis(diethylamino)-6-hydrazino-1,3,5-triazine	668:715	Oligosaccharides are easily derivatized by 2,4-bis(diethylamino)-6-hydrazino-1,3,5-triazine under convenient and mild conditions.
25828508	3	48	theme	novel	341:345	arg1	method					374:379	a novel oligosaccharide analytical method	339:379	a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure	339:542	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	4	49	theme	convenient	723:732	arg1	conditions					743:752	convenient and mild conditions	723:752	convenient and mild conditions	723:752	Oligosaccharides are easily derivatized by 2,4-bis(diethylamino)-6-hydrazino-1,3,5-triazine under convenient and mild conditions.
25828508	2	50	theme	quality	278:284	arg1	control					286:292	quality control	278:292	quality control	278:292	However, the complexity of oligosaccharides seriously challenges their profiling, quality control, and elucidation of activity.
25828508	7	51	theme	Epimedium	1107:1115	arg1	samples					1117:1123	52 Epimedium samples	1104:1123	52 Epimedium samples	1104:1123	Oligosaccharides of 52 Epimedium samples were profiled and identified based on the high-resolution mass spectral data.
25828508	4	52	theme	mild	738:741	arg1	conditions					743:752	convenient and mild conditions	723:752	convenient and mild conditions	723:752	Oligosaccharides are easily derivatized by 2,4-bis(diethylamino)-6-hydrazino-1,3,5-triazine under convenient and mild conditions.
25828508	3	53	theme	oligosaccharide	347:361	arg1	method					374:379	a novel oligosaccharide analytical method	339:379	a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure	339:542	In this paper, a novel oligosaccharide analytical method based on a new derivatization pretreatment and ultra-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HRMS) procedure was developed to rapidly profile and identify the oligosaccharides of Epimedium.
25828508	6	54	theme	magnitude	1028:1036	arg1	orders					1018:1023	the MS two orders	1007:1023	the MS two orders of magnitude higher than that of the free oligosaccharide	1007:1081	Benefitting from the derivatization, the oligosaccharides generated a response in the MS two orders of magnitude higher than that of the free oligosaccharide.
25828508	10	55	theme	oligosaccharide	1499:1513	arg1	compounds					1515:1523	Thirteen oligosaccharide compounds	1490:1523	Thirteen oligosaccharide compounds	1490:1523	Thirteen oligosaccharide compounds demonstrated potential as markers for Epimedium species classification, and their structures were preliminarily identified using MS/MS spectra.
25828508	1	56	theme	diverse	150:156	arg1	activities					184:193	diverse important pharmacological activities	150:193	diverse important pharmacological activities	150:193	Oligosaccharides, which exist widely in herbs, present diverse important pharmacological activities.
25828508	4	57	theme	diethylamino	676:687	arg1	-6-hydrazino-1,3,5-triazine					689:715	2,4-bis(diethylamino)-6-hydrazino-1,3,5-triazine	668:715	2,4-bis(diethylamino)-6-hydrazino-1,3,5-triazine	668:715	Oligosaccharides are easily derivatized by 2,4-bis(diethylamino)-6-hydrazino-1,3,5-triazine under convenient and mild conditions.
27214784	3	0	theme	swelling	488:495	arg1	morphology					431:440	The morphology	427:440	The morphology	427:440	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	0	theme	swelling	488:495	arg1	behavior					497:504	thermal and swelling behavior	476:504	thermal and swelling behavior	476:504	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	1	1	theme	oil	259:261	arg1	emulsions					269:277	stabilized olive/silicone oil Janus emulsions	233:277	stabilized olive/silicone oil Janus emulsions	233:277	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	5	2	theme	pore	894:897	arg1	size					899:902	the pore size	890:902	the pore size	890:902	Besides, the incorporation of nanoporous calcium phosphate balls is also lowering the pore size and enhancing thermal stability.
27214784	1	3	theme	Janus	263:267	arg1	emulsions					269:277	stabilized olive/silicone oil Janus emulsions	233:277	stabilized olive/silicone oil Janus emulsions	233:277	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	3	4	theme	Electron	530:537	arg1	SEM					551:553	SEM	551:553	SEM	551:553	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	4	theme	Electron	530:537	arg1	Fourier					557:563	Fourier	557:563	Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC)	557:658	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	4	theme	Electron	530:537	arg1	Microscopy					539:548	Scanning Electron Microscopy	521:548	Scanning Electron Microscopy (SEM)	521:554	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	4	5	theme	sponges	727:733	arg1	size					706:709	the pore size	697:709	the pore size of the GC based sponges after freeze-drying	697:753	Microscopic analysis confirmed that the pore size of the GC based sponges after freeze-drying may be drastically reduced by using Janus emulsions.
27214784	2	6	dep	washed	353:358	arg1	out					360:362	out	360:362	out	360:362	The emulsions were freeze-dried, and the oil droplets were washed out in order to prepare porous scaffolds with larger surface area.
27214784	1	7	theme	dimensional	65:75	arg1	structure					96:104	A three dimensional biopolymer network structure	57:104	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls	57:164	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	5	8	theme	calcium	849:855	arg1	balls					867:871	nanoporous calcium phosphate balls	838:871	nanoporous calcium phosphate balls	838:871	Besides, the incorporation of nanoporous calcium phosphate balls is also lowering the pore size and enhancing thermal stability.
27214784	3	9	theme	thermal	476:482	arg1	morphology					431:440	The morphology	427:440	The morphology	427:440	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	9	theme	thermal	476:482	arg1	behavior					497:504	thermal and swelling behavior	476:504	thermal and swelling behavior	476:504	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	10	theme	chemical	454:461	arg1	morphology					431:440	The morphology	427:440	The morphology	427:440	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	10	theme	chemical	454:461	arg1	composition					463:473	chemical composition	454:473	chemical composition	454:473	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	1	11	theme	biopolymer	77:86	arg1	structure					96:104	A three dimensional biopolymer network structure	57:104	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls	57:164	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	4	12	theme	Microscopic	661:671	arg1	analysis					673:680	Microscopic analysis	661:680	Microscopic analysis	661:680	Microscopic analysis confirmed that the pore size of the GC based sponges after freeze-drying may be drastically reduced by using Janus emulsions.
27214784	3	13	dep	Fourier	557:563	arg1	Transform					565:573	Transform	565:573	Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC)	565:658	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	1	14	theme	network	88:94	arg1	structure					96:104	A three dimensional biopolymer network structure	57:104	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls	57:164	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	2	15	theme	porous	384:389	arg1	scaffolds					391:399	porous scaffolds	384:399	porous scaffolds	384:399	The emulsions were freeze-dried, and the oil droplets were washed out in order to prepare porous scaffolds with larger surface area.
27214784	4	16	theme	based	721:725	arg1	sponges					727:733	the GC based sponges	714:733	the GC based sponges after freeze-drying	714:753	Microscopic analysis confirmed that the pore size of the GC based sponges after freeze-drying may be drastically reduced by using Janus emulsions.
27214784	1	17	theme	gelatin-chitosan	190:205	arg1	blend					220:224	gelatin-chitosan (GC) polymer blend	190:224	gelatin-chitosan (GC) polymer blend	190:224	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	0	18	theme	Janus	0:4	arg1	emulsion					6:13	Janus emulsion	0:13	Janus emulsion	0:13	Janus emulsion mediated porous scaffold bio-fabrication.
27214784	4	19	theme	GC	718:719	arg1	sponges					727:733	the GC based sponges	714:733	the GC based sponges after freeze-drying	714:753	Microscopic analysis confirmed that the pore size of the GC based sponges after freeze-drying may be drastically reduced by using Janus emulsions.
27214784	1	20	with	structure	96:104	arg1	balls					160:164	incorporated nano-porous calcium phosphate (CaP) balls	111:164	incorporated nano-porous calcium phosphate (CaP) balls	111:164	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	4	21	theme	Janus	791:795	arg1	emulsions					797:805	Janus emulsions	791:805	Janus emulsions	791:805	Microscopic analysis confirmed that the pore size of the GC based sponges after freeze-drying may be drastically reduced by using Janus emulsions.
27214784	1	22	theme	incorporated	111:122	arg1	balls					160:164	incorporated nano-porous calcium phosphate (CaP) balls	111:164	incorporated nano-porous calcium phosphate (CaP) balls	111:164	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	1	23	theme	GC	208:209	arg1	blend					220:224	gelatin-chitosan (GC) polymer blend	190:224	gelatin-chitosan (GC) polymer blend	190:224	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	1	24	theme	nano-porous	124:134	arg1	CaP					155:157	CaP	155:157	CaP	155:157	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	1	24	theme	nano-porous	124:134	arg1	phosphate					144:152	nano-porous calcium phosphate	124:152	incorporated nano-porous calcium phosphate (CaP) balls	111:164	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	2	25	theme	surface	413:419	arg1	area					421:424	larger surface area	406:424	larger surface area	406:424	The emulsions were freeze-dried, and the oil droplets were washed out in order to prepare porous scaffolds with larger surface area.
27214784	4	26	theme	pore	701:704	arg1	size					706:709	the pore size	697:709	the pore size of the GC based sponges after freeze-drying	697:753	Microscopic analysis confirmed that the pore size of the GC based sponges after freeze-drying may be drastically reduced by using Janus emulsions.
27214784	2	27	theme	larger	406:411	arg1	area					421:424	larger surface area	406:424	larger surface area	406:424	The emulsions were freeze-dried, and the oil droplets were washed out in order to prepare porous scaffolds with larger surface area.
27214784	3	28	theme	Infrared	575:582	arg1	FTIR					598:601	FTIR	598:601	FTIR	598:601	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	28	theme	Infrared	575:582	arg1	Spectroscopy					584:595	Infrared Spectroscopy	575:595	Infrared Spectroscopy (FTIR)	575:602	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	1	29	theme	polymer	212:218	arg1	blend					220:224	gelatin-chitosan (GC) polymer blend	190:224	gelatin-chitosan (GC) polymer blend	190:224	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	0	30	theme	scaffold	31:38	arg1	bio-fabrication					40:54	porous scaffold bio-fabrication	24:54	porous scaffold bio-fabrication	24:54	Janus emulsion mediated porous scaffold bio-fabrication.
27214784	5	31	theme	balls	867:871	arg1	incorporation					821:833	the incorporation	817:833	the incorporation of nanoporous calcium phosphate balls	817:871	Besides, the incorporation of nanoporous calcium phosphate balls is also lowering the pore size and enhancing thermal stability.
27214784	3	32	theme	micro-Differential	608:625	arg1	Calorimetry					636:646	micro-Differential Scanning Calorimetry	608:646	micro-Differential Scanning Calorimetry (micro-DSC)	608:658	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	32	theme	micro-Differential	608:625	arg1	micro-DSC					649:657	micro-DSC	649:657	micro-DSC	649:657	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	0	33	theme	porous	24:29	arg1	bio-fabrication					40:54	porous scaffold bio-fabrication	24:54	porous scaffold bio-fabrication	24:54	Janus emulsion mediated porous scaffold bio-fabrication.
27214784	5	34	theme	nanoporous	838:847	arg1	balls					867:871	nanoporous calcium phosphate balls	838:871	nanoporous calcium phosphate balls	838:871	Besides, the incorporation of nanoporous calcium phosphate balls is also lowering the pore size and enhancing thermal stability.
27214784	3	35	theme	Scanning	627:634	arg1	Calorimetry					636:646	micro-Differential Scanning Calorimetry	608:646	micro-Differential Scanning Calorimetry (micro-DSC)	608:658	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	35	theme	Scanning	627:634	arg1	micro-DSC					649:657	micro-DSC	649:657	micro-DSC	649:657	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	1	36	theme	calcium	136:142	arg1	CaP					155:157	CaP	155:157	CaP	155:157	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	1	36	theme	calcium	136:142	arg1	phosphate					144:152	nano-porous calcium phosphate	124:152	incorporated nano-porous calcium phosphate (CaP) balls	111:164	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	1	37	theme	phosphate	144:152	arg1	balls					160:164	incorporated nano-porous calcium phosphate (CaP) balls	111:164	incorporated nano-porous calcium phosphate (CaP) balls	111:164	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	2	38	theme	oil	335:337	arg1	droplets					339:346	the oil droplets	331:346	the oil droplets	331:346	The emulsions were freeze-dried, and the oil droplets were washed out in order to prepare porous scaffolds with larger surface area.
27214784	3	39	theme	Scanning	521:528	arg1	SEM					551:553	SEM	551:553	SEM	551:553	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	39	theme	Scanning	521:528	arg1	Fourier					557:563	Fourier	557:563	Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC)	557:658	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	39	theme	Scanning	521:528	arg1	Microscopy					539:548	Scanning Electron Microscopy	521:548	Scanning Electron Microscopy (SEM)	521:554	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	1	40	dep	blend	220:224	arg1	emulsions					269:277	stabilized olive/silicone oil Janus emulsions	233:277	stabilized olive/silicone oil Janus emulsions	233:277	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	5	41	theme	thermal	918:924	arg1	stability					926:934	thermal stability	918:934	thermal stability	918:934	Besides, the incorporation of nanoporous calcium phosphate balls is also lowering the pore size and enhancing thermal stability.
27214784	3	42	theme	pore	443:446	arg1	morphology					431:440	The morphology	427:440	The morphology	427:440	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	3	42	theme	pore	443:446	arg1	size					448:451	pore size	443:451	pore size	443:451	The morphology, pore size, chemical composition, thermal and swelling behavior was studied by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and micro-Differential Scanning Calorimetry (micro-DSC).
27214784	5	43	theme	phosphate	857:865	arg1	balls					867:871	nanoporous calcium phosphate balls	838:871	nanoporous calcium phosphate balls	838:871	Besides, the incorporation of nanoporous calcium phosphate balls is also lowering the pore size and enhancing thermal stability.
27214784	1	44	theme	stabilized	233:242	arg1	emulsions					269:277	stabilized olive/silicone oil Janus emulsions	233:277	stabilized olive/silicone oil Janus emulsions	233:277	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
27214784	1	45	theme	olive/silicone	244:257	arg1	emulsions					269:277	stabilized olive/silicone oil Janus emulsions	233:277	stabilized olive/silicone oil Janus emulsions	233:277	A three dimensional biopolymer network structure with incorporated nano-porous calcium phosphate (CaP) balls was fabricated by using gelatin-chitosan (GC) polymer blend and GC stabilized olive/silicone oil Janus emulsions, respectively.
25902974	6	0	theme	lime	762:765	arg1	profile					779:785	the lime tree nectar profile	758:785	the lime tree nectar profile	758:785	Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences.
25902974	10	1	theme	production	1278:1287	arg1	stages					1289:1294	analyzed production stages	1269:1294	analyzed production stages of lime honey	1269:1308	Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
25902974	10	2	from	different	1256:1264	arg1	stages					1289:1294	analyzed production stages	1269:1294	analyzed production stages of lime honey	1269:1308	Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
25902974	8	3	theme	Union	1084:1088	arg1	legislation					1090:1100	European Union legislation	1075:1100	European Union legislation	1075:1100	Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation.
25902974	10	4	theme	analyzed	1269:1276	arg1	stages					1289:1294	analyzed production stages	1269:1294	analyzed production stages of lime honey	1269:1308	Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
25902974	10	5	with	gentiobiose	1217:1227	arg1	content					1243:1249	turanose content	1234:1249	turanose content	1234:1249	Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
25902974	8	6	theme	samples	1016:1022	arg1	Fructose					947:954	Fructose	947:954	Fructose	947:954	Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation.
25902974	8	6	theme	samples	1016:1022	arg1	constituents					983:994	the major constituents	973:994	the major constituents of all investigated samples	973:1022	Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation.
25902974	8	6	theme	samples	1016:1022	arg1	limits					1053:1058	the limits	1049:1058	the limits established by European Union legislation	1049:1100	Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation.
25902974	8	6	theme	samples	1016:1022	arg1	glucose					960:966	glucose	960:966	glucose	960:966	Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation.
25902974	3	7	theme	analyzed	393:400	arg1	samples					402:408	all analyzed samples	389:408	all analyzed samples	389:408	Melissopalynological analysis confirmed domination of Tilia nectar in all analyzed samples.
25902974	1	8	from	characterization	151:166	arg1	Serbia					187:192	Serbia	187:192	Serbia	187:192	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	2	9	theme	lime	286:289	arg1	honey					291:295	ripe and unripe lime honey and lime tree nectar	270:316	honey	291:295	Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar.
25902974	1	10	theme	sugar	104:108	arg1	basis					141:145	the basis	137:145	the basis for characterization of lime honey from Serbia	137:192	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	1	10	theme	sugar	104:108	arg1	content					110:116	the sugar content	100:116	the sugar content	100:116	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	1	10	theme	sugar	104:108	arg1	Composition					62:72	Composition	62:72	Composition of phenolic compounds	62:94	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	2	11	theme	tree	306:309	arg1	nectar					311:316	ripe and unripe lime honey and lime tree nectar	270:316	nectar	311:316	Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar.
25902974	5	12	theme	high-performance	611:626	arg1	chromatography					643:656	high-performance anion-exchange chromatography	611:656	high-performance anion-exchange chromatography with amperometric detection	611:684	Sugar content was determined using high-performance anion-exchange chromatography with amperometric detection.
25902974	2	13	theme	unripe	279:284	arg1	honey					291:295	ripe and unripe lime honey and lime tree nectar	270:316	honey	291:295	Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar.
25902974	7	14	theme	ripe	919:922	arg1	honey					940:944	both ripe and unripe lime honey	914:944	both ripe and unripe lime honey	914:944	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	9	15	theme	nectar	1126:1131	arg1	sample					1133:1138	the nectar sample	1122:1138	the nectar sample	1122:1138	Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples.
25902974	4	16	theme	Phenolic	411:418	arg1	acids					420:424	Phenolic acids	411:424	Phenolic acids	411:424	Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC-OrbiTrap).
25902974	8	17	theme	major	977:981	arg1	Fructose					947:954	Fructose	947:954	Fructose	947:954	Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation.
25902974	8	17	theme	major	977:981	arg1	constituents					983:994	the major constituents	973:994	the major constituents of all investigated samples	973:1022	Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation.
25902974	8	17	theme	major	977:981	arg1	glucose					960:966	glucose	960:966	glucose	960:966	Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation.
25902974	7	18	theme	galangin	888:895	arg1	chrysin					862:868	chrysin	862:868	chrysin	862:868	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	18	theme	galangin	888:895	arg1	amount					852:857	a high amount	845:857	a high amount of chrysin, pinocembrin, and galangin	845:895	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	18	theme	galangin	888:895	arg1	pinocembrin					871:881	pinocembrin	871:881	pinocembrin	871:881	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	18	theme	galangin	888:895	arg1	galangin					888:895	galangin	888:895	galangin	888:895	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	1	19	theme	phenolic	77:84	arg1	compounds					86:94	phenolic compounds	77:94	phenolic compounds	77:94	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	2	20	from	differences	229:239	arg1	profiles					258:265	phytochemical profiles	244:265	phytochemical profiles of ripe and unripe lime honey and lime tree nectar	244:316	Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar.
25902974	1	21	theme	lime	171:174	arg1	honey					176:180	lime honey	171:180	lime honey from Serbia	171:192	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	5	22	theme	amperometric	663:674	arg1	detection					676:684	amperometric detection	663:684	amperometric detection	663:684	Sugar content was determined using high-performance anion-exchange chromatography with amperometric detection.
25902974	5	23	theme	Sugar	576:580	arg1	content					582:588	Sugar content	576:588	Sugar content	576:588	Sugar content was determined using high-performance anion-exchange chromatography with amperometric detection.
25902974	1	24	theme	compounds	86:94	arg1	basis					141:145	the basis	137:145	the basis for characterization of lime honey from Serbia	137:192	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	1	24	theme	compounds	86:94	arg1	content					110:116	the sugar content	100:116	the sugar content	100:116	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	1	24	theme	compounds	86:94	arg1	Composition					62:72	Composition	62:72	Composition of phenolic compounds	62:94	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	9	25	theme	Sucrose	1103:1109	arg1	content					1111:1117	Sucrose content	1103:1117	Sucrose content in the nectar sample	1103:1138	Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples.
25902974	1	26	theme	honey	176:180	arg1	characterization					151:166	characterization	151:166	characterization of lime honey from Serbia	151:192	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	0	27	theme	Phytochemical	0:12	arg1	fingerprints					14:25	Phytochemical fingerprints	0:25	Phytochemical fingerprints of lime honey	0:39	Phytochemical fingerprints of lime honey collected in serbia.
25902974	8	28	theme	European	1075:1082	arg1	legislation					1090:1100	European Union legislation	1075:1100	European Union legislation	1075:1100	Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation.
25902974	2	29	theme	lime	301:304	arg1	nectar					311:316	ripe and unripe lime honey and lime tree nectar	270:316	nectar	311:316	Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar.
25902974	6	30	theme	ripe	739:742	arg1	honeys					744:749	unripe and ripe honeys	728:749	unripe and ripe honeys	728:749	Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences.
25902974	0	31	theme	lime	30:33	arg1	honey					35:39	lime honey	30:39	lime honey	30:39	Phytochemical fingerprints of lime honey collected in serbia.
25902974	7	32	theme	high	847:850	arg1	chrysin					862:868	chrysin	862:868	chrysin	862:868	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	32	theme	high	847:850	arg1	amount					852:857	a high amount	845:857	a high amount of chrysin, pinocembrin, and galangin	845:895	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	32	theme	high	847:850	arg1	pinocembrin					871:881	pinocembrin	871:881	pinocembrin	871:881	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	32	theme	high	847:850	arg1	galangin					888:895	galangin	888:895	galangin	888:895	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	10	33	theme	honey	1304:1308	arg1	stages					1289:1294	analyzed production stages	1269:1294	analyzed production stages of lime honey	1269:1308	Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
25902974	5	34	theme	anion-exchange	628:641	arg1	chromatography					643:656	high-performance anion-exchange chromatography	611:656	high-performance anion-exchange chromatography with amperometric detection	611:684	Sugar content was determined using high-performance anion-exchange chromatography with amperometric detection.
25902974	10	35	from	stages	1289:1294	arg1	different					1256:1264	different	1256:1264	different	1256:1264	Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
25902974	10	36	with	Isomaltose	1202:1211	arg1	content					1243:1249	turanose content	1234:1249	turanose content	1234:1249	Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
25902974	6	37	theme	notable	794:800	arg1	differences					802:812	notable differences	794:812	notable differences	794:812	Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences.
25902974	2	38	theme	honey	291:295	arg1	profiles					258:265	phytochemical profiles	244:265	phytochemical profiles of ripe and unripe lime honey and lime tree nectar	244:316	Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar.
25902974	6	39	theme	unripe	728:733	arg1	honeys					744:749	unripe and ripe honeys	728:749	unripe and ripe honeys	728:749	Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences.
25902974	7	40	theme	chrysin	862:868	arg1	chrysin					862:868	chrysin	862:868	chrysin	862:868	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	40	theme	chrysin	862:868	arg1	amount					852:857	a high amount	845:857	a high amount of chrysin, pinocembrin, and galangin	845:895	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	40	theme	chrysin	862:868	arg1	pinocembrin					871:881	pinocembrin	871:881	pinocembrin	871:881	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	40	theme	chrysin	862:868	arg1	galangin					888:895	galangin	888:895	galangin	888:895	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	9	41	dep	two-fold	1150:1157	arg1	to					1147:1148	to	1147:1148	to	1147:1148	Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples.
25902974	9	41	dep	two-fold	1150:1157	arg1	higher					1159:1164	higher	1159:1164	higher	1159:1164	Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples.
25902974	10	42	theme	turanose	1234:1241	arg1	content					1243:1249	turanose content	1234:1249	turanose content	1234:1249	Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
25902974	0	43	theme	honey	35:39	arg1	fingerprints					14:25	Phytochemical fingerprints	0:25	Phytochemical fingerprints of lime honey	0:39	Phytochemical fingerprints of lime honey collected in serbia.
25902974	7	44	theme	lime	827:830	arg1	nectar					837:842	lime tree nectar	827:842	lime tree nectar	827:842	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	4	45	theme	flavonoid	458:466	arg1	glycosides					468:477	flavonoid glycosides	458:477	flavonoid glycosides	458:477	Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC-OrbiTrap).
25902974	3	46	theme	nectar	379:384	arg1	domination					359:368	domination	359:368	domination of Tilia nectar	359:384	Melissopalynological analysis confirmed domination of Tilia nectar in all analyzed samples.
25902974	10	47	theme	lime	1299:1302	arg1	honey					1304:1308	lime honey	1299:1308	lime honey	1299:1308	Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
25902974	7	48	theme	tree	832:835	arg1	nectar					837:842	lime tree nectar	827:842	lime tree nectar	827:842	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	4	49	theme	mass	540:543	arg1	UHPLC-OrbiTrap					559:572	UHPLC-OrbiTrap	559:572	UHPLC-OrbiTrap	559:572	Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC-OrbiTrap).
25902974	4	49	theme	mass	540:543	arg1	spectrometer					545:556	a hybrid mass spectrometer	531:556	a hybrid mass spectrometer (UHPLC-OrbiTrap)	531:573	Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC-OrbiTrap).
25902974	4	50	theme	abscisic	427:434	arg1	acid					436:439	abscisic acid	427:439	abscisic acid	427:439	Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC-OrbiTrap).
25902974	1	51	from	Serbia	187:192	arg1	honey					176:180	lime honey	171:180	lime honey from Serbia	171:192	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	1	51	from	Serbia	187:192	arg1	characterization					151:166	characterization	151:166	characterization of lime honey from Serbia	151:192	Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia.
25902974	7	52	located	detected	902:909	arg2	galangin					888:895	galangin	888:895	galangin	888:895	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	52	located	detected	902:909	arg2	amount					852:857	a high amount	845:857	a high amount of chrysin, pinocembrin, and galangin	845:895	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	52	located	detected	902:909	arg1	honey					940:944	both ripe and unripe lime honey	914:944	both ripe and unripe lime honey	914:944	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	52	located	detected	902:909	arg2	pinocembrin					871:881	pinocembrin	871:881	pinocembrin	871:881	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	52	located	detected	902:909	arg2	chrysin					862:868	chrysin	862:868	chrysin	862:868	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	53	theme	unripe	928:933	arg1	honey					940:944	both ripe and unripe lime honey	914:944	both ripe and unripe lime honey	914:944	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	4	54	theme	hybrid	533:538	arg1	UHPLC-OrbiTrap					559:572	UHPLC-OrbiTrap	559:572	UHPLC-OrbiTrap	559:572	Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC-OrbiTrap).
25902974	4	54	theme	hybrid	533:538	arg1	spectrometer					545:556	a hybrid mass spectrometer	531:556	a hybrid mass spectrometer (UHPLC-OrbiTrap)	531:573	Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC-OrbiTrap).
25902974	2	55	theme	Particular	195:204	arg1	attention					206:214	Particular attention	195:214	Particular attention	195:214	Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar.
25902974	8	56	theme	investigated	1003:1014	arg1	samples					1016:1022	all investigated samples	999:1022	all investigated samples	999:1022	Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation.
25902974	9	57	theme	honey	1187:1191	arg1	samples					1193:1199	all honey samples	1183:1199	all honey samples	1183:1199	Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples.
25902974	5	58	with	chromatography	643:656	arg1	detection					676:684	amperometric detection	663:684	amperometric detection	663:684	Sugar content was determined using high-performance anion-exchange chromatography with amperometric detection.
25902974	6	59	theme	phenolic	695:702	arg1	compounds					704:712	Similar phenolic compounds	687:712	Similar phenolic compounds	687:712	Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences.
25902974	2	60	theme	ripe	270:273	arg1	honey					291:295	ripe and unripe lime honey and lime tree nectar	270:316	honey	291:295	Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar.
25902974	9	61	from	content	1111:1117	arg1	sample					1133:1138	the nectar sample	1122:1138	the nectar sample	1122:1138	Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples.
25902974	2	62	theme	nectar	311:316	arg1	profiles					258:265	phytochemical profiles	244:265	phytochemical profiles of ripe and unripe lime honey and lime tree nectar	244:316	Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar.
25902974	6	63	theme	Similar	687:693	arg1	compounds					704:712	Similar phenolic compounds	687:712	Similar phenolic compounds	687:712	Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences.
25902974	7	64	theme	pinocembrin	871:881	arg1	chrysin					862:868	chrysin	862:868	chrysin	862:868	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	64	theme	pinocembrin	871:881	arg1	amount					852:857	a high amount	845:857	a high amount of chrysin, pinocembrin, and galangin	845:895	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	64	theme	pinocembrin	871:881	arg1	pinocembrin					871:881	pinocembrin	871:881	pinocembrin	871:881	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	7	64	theme	pinocembrin	871:881	arg1	galangin					888:895	galangin	888:895	galangin	888:895	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	3	65	theme	Melissopalynological	319:338	arg1	analysis					340:347	Melissopalynological analysis	319:347	Melissopalynological analysis	319:347	Melissopalynological analysis confirmed domination of Tilia nectar in all analyzed samples.
25902974	2	66	theme	phytochemical	244:256	arg1	profiles					258:265	phytochemical profiles	244:265	phytochemical profiles of ripe and unripe lime honey and lime tree nectar	244:316	Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar.
25902974	6	67	theme	nectar	772:777	arg1	profile					779:785	the lime tree nectar profile	758:785	the lime tree nectar profile	758:785	Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences.
25902974	7	68	theme	lime	935:938	arg1	honey					940:944	both ripe and unripe lime honey	914:944	both ripe and unripe lime honey	914:944	Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey.
25902974	6	69	theme	tree	767:770	arg1	profile					779:785	the lime tree nectar profile	758:785	the lime tree nectar profile	758:785	Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences.
25902974	3	70	theme	Tilia	373:377	arg1	nectar					379:384	Tilia nectar	373:384	Tilia nectar	373:384	Melissopalynological analysis confirmed domination of Tilia nectar in all analyzed samples.
25435606	7	0	theme	protein	1337:1343	arg1	content					1345:1351	protein content	1337:1351	protein content	1337:1351	RESULTS Flour showed higher (P < 0.05), moisture content, fat, carbohydrate, amylopectine, and protein content than starch, while amylose content of this starch was higher (P<0.05).
25435606	8	1	dep	Phosphorus	1424:1433	arg1	minerals					1469:1476	minerals	1469:1476	minerals	1469:1476	Phosphorus, sodium, magnesium, and potassium minerals content were higher in flour than starch.
25435606	6	2	theme	test	1170:1173	arg1	results					1175:1181	test results	1170:1181	test results	1170:1181	Gelatinization characteristics and in vitro starch digestibility were also determined, test results were processed using one-way analysis of variance (ANOVA).
25435606	5	3	theme	extract	1074:1080	arg1	property					1045:1052	the crystalline property	1029:1052	the crystalline property of flour and starch extract	1029:1080	Pasting properties was determined and X-ray powder starch diffraction was used to examine the crystalline property of flour and starch extract.
25435606	4	4	theme	AOAC	920:923	arg1	method					910:915	the official method	897:915	the official method of AOAC	897:923	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	4	theme	AOAC	920:923	arg1	spectrocolorimeter					873:890	color flex spectrocolorimeter	862:890	color flex spectrocolorimeter	862:890	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	5	theme	different	546:554	arg1	methods					556:562	Three different methods	540:562	Three different methods	540:562	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	10	6	theme	final	1734:1738	arg1	viscosity					1740:1748	final viscosity	1734:1748	final viscosity	1734:1748	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	3	7	theme	extract	447:453	arg1	characteristics					385:399	mineral characteristics	377:399	mineral characteristics	377:399	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	3	7	theme	extract	447:453	arg1	properties					416:425	functional properties	405:425	functional properties	405:425	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	11	8	theme	native	1902:1907	arg1	biglobosa					1916:1924	native Parkia biglobosa	1902:1924	native Parkia biglobosa grown in Guinea under controlled environmental conditions	1902:1982	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	11	9	theme	extract	2041:2047	arg1	starch					2056:2061	the extract Parkia starch	2037:2061	the extract Parkia starch	2037:2061	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	11	9	theme	extract	2041:2047	arg1	material					2085:2092	an ideal SDS material	2072:2092	an ideal SDS material	2072:2092	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	10	10	theme	viscosity	1723:1731	arg1	decrease					1711:1718	a decrease	1709:1718	a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour	1709:1809	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	1	11	theme	African	166:172	arg1	locust					174:179	BACKGROUND African locust	155:179	BACKGROUND African locust bean tree	155:189	BACKGROUND African locust bean tree is an important food tree for both human and livestock such as husks and pods.
25435606	4	12	theme	Starch	616:621	arg1	%					630:630	%	630:630	%	630:630	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	12	theme	Starch	616:621	arg1	yield					623:627	the Starch yield	612:627	the Starch yield (%)	612:631	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	12	theme	Starch	616:621	arg1	analysis					645:652	composition analysis	633:652	composition analysis for	633:656	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	6	13	theme	starch	1127:1132	arg1	digestibility					1134:1146	in vitro starch digestibility	1118:1146	in vitro starch digestibility	1118:1146	Gelatinization characteristics and in vitro starch digestibility were also determined, test results were processed using one-way analysis of variance (ANOVA).
25435606	1	14	theme	bean	181:184	arg1	tree					212:215	an important food tree	194:215	an important food tree for both human and livestock such as husks and pods	194:267	BACKGROUND African locust bean tree is an important food tree for both human and livestock such as husks and pods.
25435606	1	14	theme	bean	181:184	arg1	tree					186:189	BACKGROUND African locust bean tree	155:189	BACKGROUND African locust bean tree	155:189	BACKGROUND African locust bean tree is an important food tree for both human and livestock such as husks and pods.
25435606	4	15	theme	AACC	795:798	arg1	method					800:805	AACC method	795:805	AACC method	795:805	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	7	16	theme	P	1271:1271	arg1	<					1273:1273	P < 0.05	1271:1278	P < 0.05	1271:1278	RESULTS Flour showed higher (P < 0.05), moisture content, fat, carbohydrate, amylopectine, and protein content than starch, while amylose content of this starch was higher (P<0.05).
25435606	6	17	theme	variance	1224:1231	arg1	analysis					1212:1219	one-way analysis	1204:1219	one-way analysis of variance (ANOVA)	1204:1239	Gelatinization characteristics and in vitro starch digestibility were also determined, test results were processed using one-way analysis of variance (ANOVA).
25435606	4	18	from	extraction	585:594	arg1	other					599:603	other	599:603	other	599:603	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	0	19	from	extract	76:82	arg1	methods					97:103	different methods	87:103	different methods	87:103	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	7	20	theme	higher	1263:1268	arg1	content					1291:1297	moisture content	1282:1297	moisture content	1282:1297	RESULTS Flour showed higher (P < 0.05), moisture content, fat, carbohydrate, amylopectine, and protein content than starch, while amylose content of this starch was higher (P<0.05).
25435606	8	21	theme	magnesium	1444:1452	arg1	content					1478:1484	Phosphorus, sodium, magnesium, and potassium minerals content	1424:1484	Phosphorus, sodium, magnesium, and potassium minerals content	1424:1484	Phosphorus, sodium, magnesium, and potassium minerals content were higher in flour than starch.
25435606	9	22	theme	absorption	1588:1597	arg1	capacity					1599:1606	oil absorption capacity	1584:1606	oil absorption capacity content of the flour	1584:1627	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	1	23	theme	important	197:205	arg1	tree					212:215	an important food tree	194:215	an important food tree for both human and livestock such as husks and pods	194:267	BACKGROUND African locust bean tree is an important food tree for both human and livestock such as husks and pods.
25435606	1	23	theme	important	197:205	arg1	tree					186:189	BACKGROUND African locust bean tree	155:189	BACKGROUND African locust bean tree	155:189	BACKGROUND African locust bean tree is an important food tree for both human and livestock such as husks and pods.
25435606	4	24	theme	composition	633:643	arg1	yield					623:627	the Starch yield	612:627	the Starch yield (%)	612:631	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	24	theme	composition	633:643	arg1	analysis					645:652	composition analysis	633:652	composition analysis for	633:656	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	6	25	theme	Gelatinization	1083:1096	arg1	characteristics					1098:1112	Gelatinization characteristics	1083:1112	Gelatinization characteristics	1083:1112	Gelatinization characteristics and in vitro starch digestibility were also determined, test results were processed using one-way analysis of variance (ANOVA).
25435606	9	26	theme	pH	1563:1564	arg1	properties					1528:1537	Pasting properties	1520:1537	Pasting properties	1520:1537	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	26	theme	pH	1563:1564	arg1	values					1566:1571	pH values	1563:1571	pH values	1563:1571	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	10	27	theme	pasting	1782:1788	arg1	temperature					1790:1800	pasting temperature	1782:1800	pasting temperature	1782:1800	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	8	28	theme	potassium	1459:1467	arg1	content					1478:1484	Phosphorus, sodium, magnesium, and potassium minerals content	1424:1484	Phosphorus, sodium, magnesium, and potassium minerals content	1424:1484	Phosphorus, sodium, magnesium, and potassium minerals content were higher in flour than starch.
25435606	5	29	theme	starch	990:995	arg1	diffraction					997:1007	X-ray powder starch diffraction	977:1007	X-ray powder starch diffraction	977:1007	Pasting properties was determined and X-ray powder starch diffraction was used to examine the crystalline property of flour and starch extract.
25435606	11	30	theme	ideal	2010:2014	arg1	flour					1891:1895	flour	1891:1895	flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions	1891:1982	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	11	30	theme	ideal	2010:2014	arg1	material					2019:2026	an ideal RS material	2007:2026	an ideal RS material	2007:2026	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	11	31	from	biglobosa	1916:1924	arg1	flour					1891:1895	flour	1891:1895	flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions	1891:1982	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	11	31	from	biglobosa	1916:1924	arg1	material					2019:2026	an ideal RS material	2007:2026	an ideal RS material	2007:2026	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	5	32	used	used	1013:1016	arg2	diffraction					997:1007	X-ray powder starch diffraction	977:1007	X-ray powder starch diffraction	977:1007	Pasting properties was determined and X-ray powder starch diffraction was used to examine the crystalline property of flour and starch extract.
25435606	0	33	theme	extract	76:82	arg1	properties					45:54	physicochemical and functional properties	14:54	physicochemical and functional properties of flour and starch extract in different methods	14:103	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	4	34	from	ash	683:685	arg1	biglobosa					747:755	Parkia biglobosa	740:755	Parkia biglobosa	740:755	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	11	35	theme	RS	2016:2017	arg1	flour					1891:1895	flour	1891:1895	flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions	1891:1982	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	11	35	theme	RS	2016:2017	arg1	material					2019:2026	an ideal RS material	2007:2026	an ideal RS material	2007:2026	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	0	36	theme	different	87:95	arg1	methods					97:103	different methods	87:103	different methods	87:103	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	4	37	dep	MATERIALS	518:526	arg1	methods					556:562	Three different methods	540:562	Three different methods	540:562	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	38	from	fat	678:680	arg1	biglobosa					747:755	Parkia biglobosa	740:755	Parkia biglobosa	740:755	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	7	39	theme	starch	1396:1401	arg1	content					1380:1386	amylose content	1372:1386	amylose content of this starch	1372:1401	RESULTS Flour showed higher (P < 0.05), moisture content, fat, carbohydrate, amylopectine, and protein content than starch, while amylose content of this starch was higher (P<0.05).
25435606	4	40	theme	value	821:825	arg1	measurements					827:838	PH value measurements	818:838	PH value measurements	818:838	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	5	41	theme	X-ray	977:981	arg1	diffraction					997:1007	X-ray powder starch diffraction	977:1007	X-ray powder starch diffraction	977:1007	Pasting properties was determined and X-ray powder starch diffraction was used to examine the crystalline property of flour and starch extract.
25435606	11	42	theme	environmental	1959:1971	arg1	conditions					1973:1982	controlled environmental conditions	1948:1982	controlled environmental conditions	1948:1982	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	4	43	from	protein	669:675	arg1	biglobosa					747:755	Parkia biglobosa	740:755	Parkia biglobosa	740:755	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	44	dep	yield	623:627	arg1	contents					697:704	fiber contents	691:704	fiber contents	691:704	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	44	dep	yield	623:627	arg1	fat					678:680	fat	678:680	fat	678:680	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	44	dep	yield	623:627	arg1	protein					669:675	protein	669:675	protein	669:675	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	44	dep	yield	623:627	arg1	moisture					659:666	moisture	659:666	moisture	659:666	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	44	dep	yield	623:627	arg1	ash					683:685	ash	683:685	ash	683:685	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	45	from	moisture	659:666	arg1	biglobosa					747:755	Parkia biglobosa	740:755	Parkia biglobosa	740:755	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	46	theme	official	901:908	arg1	method					910:915	the official method	897:915	the official method of AOAC	897:923	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	12	47	theme	interesting	2125:2135	arg1	alternative					2137:2147	an interesting alternative	2122:2147	an interesting alternative for food developers	2122:2167	Therefore, these may offer an interesting alternative for food developers, depending on their characteristics and functional properties.
25435606	3	48	theme	functional	405:414	arg1	properties					416:425	functional properties	405:425	functional properties	405:425	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	0	49	theme	physicochemical	14:28	arg1	properties					45:54	physicochemical and functional properties	14:54	physicochemical and functional properties of flour and starch extract in different methods	14:103	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	5	50	theme	flour	1057:1061	arg1	property					1045:1052	the crystalline property	1029:1052	the crystalline property of flour and starch extract	1029:1080	Pasting properties was determined and X-ray powder starch diffraction was used to examine the crystalline property of flour and starch extract.
25435606	4	51	theme	color	862:866	arg1	spectrocolorimeter					873:890	color flex spectrocolorimeter	862:890	color flex spectrocolorimeter	862:890	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	12	52	theme	functional	2209:2218	arg1	properties					2220:2229	functional properties	2209:2229	functional properties	2209:2229	Therefore, these may offer an interesting alternative for food developers, depending on their characteristics and functional properties.
25435606	11	53	dep	considered	1993:2002	arg1	whereas					2029:2035	whereas	2029:2035	whereas	2029:2035	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	0	54	theme	functional	34:43	arg1	properties					45:54	physicochemical and functional properties	14:54	physicochemical and functional properties of flour and starch extract in different methods	14:103	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	3	55	theme	study	333:337	arg1	aim					321:323	The aim	317:323	The aim of this study	317:337	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	4	56	theme	extracts	726:733	arg1	contents					697:704	fiber contents	691:704	fiber contents	691:704	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	56	theme	extracts	726:733	arg1	fat					678:680	fat	678:680	fat	678:680	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	56	theme	extracts	726:733	arg1	protein					669:675	protein	669:675	protein	669:675	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	56	theme	extracts	726:733	arg1	moisture					659:666	moisture	659:666	moisture	659:666	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	56	theme	extracts	726:733	arg1	ash					683:685	ash	683:685	ash	683:685	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	11	57	theme	SDS	2081:2083	arg1	starch					2056:2061	the extract Parkia starch	2037:2061	the extract Parkia starch	2037:2061	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	11	57	theme	SDS	2081:2083	arg1	material					2085:2092	an ideal SDS material	2072:2092	an ideal SDS material	2072:2092	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	4	58	theme	fiber	691:695	arg1	contents					697:704	fiber contents	691:704	fiber contents	691:704	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	2	59	theme	vital	286:290	arg1	role					292:295	a very vital role	279:295	a very vital role	279:295	It plays a very vital role in the rural areas.
25435606	1	60	theme	food	207:210	arg1	tree					212:215	an important food tree	194:215	an important food tree for both human and livestock such as husks and pods	194:267	BACKGROUND African locust bean tree is an important food tree for both human and livestock such as husks and pods.
25435606	1	60	theme	food	207:210	arg1	tree					186:189	BACKGROUND African locust bean tree	155:189	BACKGROUND African locust bean tree	155:189	BACKGROUND African locust bean tree is an important food tree for both human and livestock such as husks and pods.
25435606	8	61	theme	Phosphorus	1424:1433	arg1	content					1478:1484	Phosphorus, sodium, magnesium, and potassium minerals content	1424:1484	Phosphorus, sodium, magnesium, and potassium minerals content	1424:1484	Phosphorus, sodium, magnesium, and potassium minerals content were higher in flour than starch.
25435606	8	62	theme	sodium	1436:1441	arg1	content					1478:1484	Phosphorus, sodium, magnesium, and potassium minerals content	1424:1484	Phosphorus, sodium, magnesium, and potassium minerals content	1424:1484	Phosphorus, sodium, magnesium, and potassium minerals content were higher in flour than starch.
25435606	2	63	theme	rural	304:308	arg1	areas					310:314	the rural areas	300:314	the rural areas	300:314	It plays a very vital role in the rural areas.
25435606	5	64	theme	starch	1067:1072	arg1	extract					1074:1080	starch extract	1067:1080	starch extract	1067:1080	Pasting properties was determined and X-ray powder starch diffraction was used to examine the crystalline property of flour and starch extract.
25435606	0	65	from	seeds	148:152	arg1	Comparison					0:9	Comparison	0:9	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.	0:153	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	11	66	dep	CONCLUSION	1844:1853	arg1	results					1864:1870	our results	1860:1870	our results	1860:1870	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	4	67	theme	dry	776:778	arg1	db					787:788	db	787:788	db	787:788	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	67	theme	dry	776:778	arg1	basis					780:784	dry basis	776:784	dry basis (db)	776:789	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	3	68	theme	biglobosa	476:484	arg1	seeds					486:490	Parkia biglobosa seeds	469:490	Parkia biglobosa seeds	469:490	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	1	69	theme	locust	174:179	arg1	tree					212:215	an important food tree	194:215	an important food tree for both human and livestock such as husks and pods	194:267	BACKGROUND African locust bean tree is an important food tree for both human and livestock such as husks and pods.
25435606	1	69	theme	locust	174:179	arg1	tree					186:189	BACKGROUND African locust bean tree	155:189	BACKGROUND African locust bean tree	155:189	BACKGROUND African locust bean tree is an important food tree for both human and livestock such as husks and pods.
25435606	1	70	theme	BACKGROUND	155:164	arg1	locust					174:179	BACKGROUND African locust	155:179	BACKGROUND African locust bean tree	155:189	BACKGROUND African locust bean tree is an important food tree for both human and livestock such as husks and pods.
25435606	11	71	theme	controlled	1948:1957	arg1	conditions					1973:1982	controlled environmental conditions	1948:1982	controlled environmental conditions	1948:1982	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	11	72	theme	Parkia	1909:1914	arg1	biglobosa					1916:1924	native Parkia biglobosa	1902:1924	native Parkia biglobosa grown in Guinea under controlled environmental conditions	1902:1982	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	7	73	theme	moisture	1282:1289	arg1	content					1291:1297	moisture content	1282:1297	moisture content	1282:1297	RESULTS Flour showed higher (P < 0.05), moisture content, fat, carbohydrate, amylopectine, and protein content than starch, while amylose content of this starch was higher (P<0.05).
25435606	7	74	dep	higher	1263:1268	arg1	<					1273:1273	P < 0.05	1271:1278	P < 0.05	1271:1278	RESULTS Flour showed higher (P < 0.05), moisture content, fat, carbohydrate, amylopectine, and protein content than starch, while amylose content of this starch was higher (P<0.05).
25435606	10	75	theme	set	1751:1753	arg1	value					1760:1764	set back value	1751:1764	set back value	1751:1764	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	0	76	theme	bean	124:127	arg1	seeds					148:152	Africa locust bean (Parkia biglobosa) seeds	110:152	Africa locust bean (Parkia biglobosa) seeds	110:152	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	3	77	theme	different	499:507	arg1	methods					509:515	different methods	499:515	different methods	499:515	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	9	78	theme	Pasting	1520:1526	arg1	color					1556:1560	color	1556:1560	color	1556:1560	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	78	theme	Pasting	1520:1526	arg1	properties					1528:1537	Pasting properties	1520:1537	Pasting properties	1520:1537	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	78	theme	Pasting	1520:1526	arg1	values					1566:1571	pH values	1563:1571	pH values	1563:1571	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	78	theme	Pasting	1520:1526	arg1	content					1608:1614	oil absorption capacity content	1584:1614	oil absorption capacity content of the flour	1584:1627	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	78	theme	Pasting	1520:1526	arg1	water					1574:1578	water	1574:1578	water	1574:1578	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	78	theme	Pasting	1520:1526	arg1	gelatinisation					1540:1553	gelatinisation	1540:1553	gelatinisation	1540:1553	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	11	79	theme	Parkia	2049:2054	arg1	starch					2056:2061	the extract Parkia starch	2037:2061	the extract Parkia starch	2037:2061	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	11	79	theme	Parkia	2049:2054	arg1	material					2085:2092	an ideal SDS material	2072:2092	an ideal SDS material	2072:2092	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	4	80	theme	starch	578:583	arg1	extraction					585:594	starch extraction	578:594	starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa	578:755	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	10	81	theme	viscosity	1740:1748	arg1	decrease					1711:1718	a decrease	1709:1718	a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour	1709:1809	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	0	82	theme	Parkia	130:135	arg1	bean					124:127	Africa locust bean	110:127	Africa locust bean (Parkia biglobosa) seeds	110:152	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	0	82	theme	Parkia	130:135	arg1	biglobosa					137:145	Parkia biglobosa	130:145	Parkia biglobosa	130:145	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	5	83	theme	Pasting	939:945	arg1	properties					947:956	Pasting properties	939:956	Pasting properties	939:956	Pasting properties was determined and X-ray powder starch diffraction was used to examine the crystalline property of flour and starch extract.
25435606	6	84	theme	in	1118:1119	arg1	digestibility					1134:1146	in vitro starch digestibility	1118:1146	in vitro starch digestibility	1118:1146	Gelatinization characteristics and in vitro starch digestibility were also determined, test results were processed using one-way analysis of variance (ANOVA).
25435606	10	85	theme	breakdown	1767:1775	arg1	decrease					1711:1718	a decrease	1709:1718	a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour	1709:1809	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	6	86	theme	one-way	1204:1210	arg1	analysis					1212:1219	one-way analysis	1204:1219	one-way analysis of variance (ANOVA)	1204:1239	Gelatinization characteristics and in vitro starch digestibility were also determined, test results were processed using one-way analysis of variance (ANOVA).
25435606	10	87	theme	value	1760:1764	arg1	decrease					1711:1718	a decrease	1709:1718	a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour	1709:1809	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	0	88	from	methods	97:103	arg1	properties					45:54	physicochemical and functional properties	14:54	physicochemical and functional properties of flour and starch extract in different methods	14:103	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	7	89	theme	RESULTS	1242:1248	arg1	Flour					1250:1254	RESULTS Flour	1242:1254	RESULTS Flour	1242:1254	RESULTS Flour showed higher (P < 0.05), moisture content, fat, carbohydrate, amylopectine, and protein content than starch, while amylose content of this starch was higher (P<0.05).
25435606	10	90	theme	temperature	1790:1800	arg1	decrease					1711:1718	a decrease	1709:1718	a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour	1709:1809	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	0	91	theme	starch	69:74	arg1	extract					76:82	starch extract	69:82	starch extract	69:82	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	7	92	dep	higher	1407:1412	arg1	P<0.05					1415:1420	P<0.05	1415:1420	P<0.05	1415:1420	RESULTS Flour showed higher (P < 0.05), moisture content, fat, carbohydrate, amylopectine, and protein content than starch, while amylose content of this starch was higher (P<0.05).
25435606	4	93	from	contents	697:704	arg1	biglobosa					747:755	Parkia biglobosa	740:755	Parkia biglobosa	740:755	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	0	94	theme	locust	117:122	arg1	bean					124:127	Africa locust bean	110:127	Africa locust bean (Parkia biglobosa) seeds	110:152	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	0	94	theme	locust	117:122	arg1	biglobosa					137:145	Parkia biglobosa	130:145	Parkia biglobosa	130:145	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	6	95	dep	in	1118:1119	arg1	vitro					1121:1125	vitro	1121:1125	vitro	1121:1125	Gelatinization characteristics and in vitro starch digestibility were also determined, test results were processed using one-way analysis of variance (ANOVA).
25435606	9	96	theme	oil	1584:1586	arg1	capacity					1599:1606	oil absorption capacity	1584:1606	oil absorption capacity content of the flour	1584:1627	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	0	97	from	flour	59:63	arg1	methods					97:103	different methods	87:103	different methods	87:103	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	10	98	theme	flour	1805:1809	arg1	temperature					1790:1800	pasting temperature	1782:1800	pasting temperature	1782:1800	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	10	98	theme	flour	1805:1809	arg1	viscosity					1723:1731	viscosity	1723:1731	viscosity	1723:1731	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	10	98	theme	flour	1805:1809	arg1	viscosity					1740:1748	final viscosity	1734:1748	final viscosity	1734:1748	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	10	98	theme	flour	1805:1809	arg1	breakdown					1767:1775	breakdown	1767:1775	breakdown	1767:1775	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	10	98	theme	flour	1805:1809	arg1	value					1760:1764	set back value	1751:1764	set back value	1751:1764	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	0	99	theme	Africa	110:115	arg1	bean					124:127	Africa locust bean	110:127	Africa locust bean (Parkia biglobosa) seeds	110:152	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	0	99	theme	Africa	110:115	arg1	biglobosa					137:145	Parkia biglobosa	130:145	Parkia biglobosa	130:145	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	4	100	from	biglobosa	747:755	arg1	contents					697:704	fiber contents	691:704	fiber contents	691:704	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	100	from	biglobosa	747:755	arg1	fat					678:680	fat	678:680	fat	678:680	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	100	from	biglobosa	747:755	arg1	flour					709:713	flour	709:713	flour	709:713	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	100	from	biglobosa	747:755	arg1	ash					683:685	ash	683:685	ash	683:685	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	100	from	biglobosa	747:755	arg1	moisture					659:666	moisture	659:666	moisture	659:666	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	100	from	biglobosa	747:755	arg1	extracts					726:733	starch extracts	719:733	starch extracts from Parkia biglobosa	719:755	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	100	from	biglobosa	747:755	arg1	protein					669:675	protein	669:675	protein	669:675	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	101	theme	PH	818:819	arg1	measurements					827:838	PH value measurements	818:838	PH value measurements	818:838	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	0	102	from	properties	45:54	arg1	methods					97:103	different methods	87:103	different methods	87:103	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	3	103	theme	mineral	377:383	arg1	characteristics					385:399	mineral characteristics	377:399	mineral characteristics	377:399	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	5	104	theme	powder	983:988	arg1	diffraction					997:1007	X-ray powder starch diffraction	977:1007	X-ray powder starch diffraction	977:1007	Pasting properties was determined and X-ray powder starch diffraction was used to examine the crystalline property of flour and starch extract.
25435606	3	105	theme	physicochemical	360:374	arg1	characteristics					385:399	mineral characteristics	377:399	mineral characteristics	377:399	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	9	106	theme	flour	1623:1627	arg1	color					1556:1560	color	1556:1560	color	1556:1560	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	106	theme	flour	1623:1627	arg1	properties					1528:1537	Pasting properties	1520:1537	Pasting properties	1520:1537	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	106	theme	flour	1623:1627	arg1	values					1566:1571	pH values	1563:1571	pH values	1563:1571	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	106	theme	flour	1623:1627	arg1	content					1608:1614	oil absorption capacity content	1584:1614	oil absorption capacity content of the flour	1584:1627	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	106	theme	flour	1623:1627	arg1	water					1574:1578	water	1574:1578	water	1574:1578	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	106	theme	flour	1623:1627	arg1	gelatinisation					1540:1553	gelatinisation	1540:1553	gelatinisation	1540:1553	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	0	107	theme	properties	45:54	arg1	Comparison					0:9	Comparison	0:9	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.	0:153	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	4	108	theme	flour	709:713	arg1	contents					697:704	fiber contents	691:704	fiber contents	691:704	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	108	theme	flour	709:713	arg1	fat					678:680	fat	678:680	fat	678:680	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	108	theme	flour	709:713	arg1	protein					669:675	protein	669:675	protein	669:675	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	108	theme	flour	709:713	arg1	moisture					659:666	moisture	659:666	moisture	659:666	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	4	108	theme	flour	709:713	arg1	ash					683:685	ash	683:685	ash	683:685	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	9	109	theme	capacity	1599:1606	arg1	properties					1528:1537	Pasting properties	1520:1537	Pasting properties	1520:1537	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	9	109	theme	capacity	1599:1606	arg1	content					1608:1614	oil absorption capacity content	1584:1614	oil absorption capacity content of the flour	1584:1627	Pasting properties, gelatinisation, color, pH values, water and oil absorption capacity content of the flour were found to be higher than that of starch.
25435606	0	110	theme	flour	59:63	arg1	properties					45:54	physicochemical and functional properties	14:54	physicochemical and functional properties of flour and starch extract in different methods	14:103	Comparison of physicochemical and functional properties of flour and starch extract in different methods from Africa locust bean (Parkia biglobosa) seeds.
25435606	7	111	theme	amylose	1372:1378	arg1	content					1380:1386	amylose content	1372:1386	amylose content of this starch	1372:1401	RESULTS Flour showed higher (P < 0.05), moisture content, fat, carbohydrate, amylopectine, and protein content than starch, while amylose content of this starch was higher (P<0.05).
25435606	4	112	theme	flex	868:871	arg1	spectrocolorimeter					873:890	color flex spectrocolorimeter	862:890	color flex spectrocolorimeter	862:890	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	10	113	theme	pasting	1678:1684	arg1	characteristics					1686:1700	The pasting characteristics	1674:1700	The pasting characteristics	1674:1700	The pasting characteristics showed a decrease of viscosity, final viscosity, set back value, breakdown, and pasting temperature of flour when compared to that of starch.
25435606	4	114	theme	starch	719:724	arg1	extracts					726:733	starch extracts	719:733	starch extracts from Parkia biglobosa	719:755	MATERIALS AND METHODS Three different methods were used for starch extraction in other to get the Starch yield (%),composition analysis for; moisture, protein, fat, ash and fiber contents of flour and starch extracts from Parkia biglobosa were determined on dry basis (db), by AACC method, color and PH value measurements was carried out using color flex spectrocolorimeter, and the official method of AOAC respectively.
25435606	3	115	theme	starch	440:445	arg1	extract					447:453	starch extract	440:453	starch extract	440:453	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	5	116	theme	crystalline	1033:1043	arg1	property					1045:1052	the crystalline property	1029:1052	the crystalline property of flour and starch extract	1029:1080	Pasting properties was determined and X-ray powder starch diffraction was used to examine the crystalline property of flour and starch extract.
25435606	3	117	dep	characteristics	385:399	arg1	some					355:358	some	355:358	some	355:358	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	3	118	theme	flour	430:434	arg1	characteristics					385:399	mineral characteristics	377:399	mineral characteristics	377:399	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	3	118	theme	flour	430:434	arg1	properties					416:425	functional properties	405:425	functional properties	405:425	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25435606	11	119	theme	ideal	2075:2079	arg1	starch					2056:2061	the extract Parkia starch	2037:2061	the extract Parkia starch	2037:2061	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	11	119	theme	ideal	2075:2079	arg1	material					2085:2092	an ideal SDS material	2072:2092	an ideal SDS material	2072:2092	CONCLUSION From our results, we speculate that flour from native Parkia biglobosa grown in Guinea under controlled environmental conditions could be considered as an ideal RS material, whereas the extract Parkia starch could be an ideal SDS material.
25435606	12	120	theme	food	2153:2156	arg1	developers					2158:2167	food developers	2153:2167	food developers	2153:2167	Therefore, these may offer an interesting alternative for food developers, depending on their characteristics and functional properties.
25435606	3	121	theme	Parkia	469:474	arg1	seeds					486:490	Parkia biglobosa seeds	469:490	Parkia biglobosa seeds	469:490	The aim of this study was to evaluate some physicochemical, mineral characteristics and functional properties of flour and starch extract produced from Parkia biglobosa seeds, using different methods.
25118690	12	0	contain	has	2066:2068	arg1	IRX14					2055:2059	IRX14	2055:2059	IRX14	2055:2059	Most likely IRX14 also has primarily a structural role, but it cannot be excluded that the protein has an important enzymatic activity.
25118690	12	0	contain	has	2066:2068	arg2	role					2093:2096	a structural role	2080:2096	a structural role	2080:2096	Most likely IRX14 also has primarily a structural role, but it cannot be excluded that the protein has an important enzymatic activity.
25118690	8	1	theme	predicted	1601:1609	arg1	acid					1627:1630	the predicted catalytic amino acid	1597:1630	the predicted catalytic amino acid	1597:1630	Two out of three mutated IRX14 complemented the irx14 mutant, including a mutant in the predicted catalytic amino acid.
25118690	2	2	theme	xylan	360:364	arg1	backbone					366:373	the xylan backbone	356:373	the xylan backbone	356:373	Several genes predicted to encode glycosyltransferases are required for the synthesis of the xylan backbone even though it is a homopolymer consisting entirely of β-1,4-linked xylose residues.
25118690	1	3	theme	plants	259:264	arg1	walls					250:254	the secondary cell walls	231:254	the secondary cell walls of plants	231:264	Xylans constitute the main non-cellulosic polysaccharide in the secondary cell walls of plants.
25118690	7	4	theme	glycosyltransferases	1399:1418	arg1	function					1420:1427	glycosyltransferases function	1399:1427	glycosyltransferases function in homologous proteins	1399:1450	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	0	5	from	function	146:153	arg1	Arabidopsis					158:168	Arabidopsis	158:168	Arabidopsis	158:168	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.
25118690	6	6	theme	wall	1147:1150	arg1	composition					1152:1162	determining cell wall composition	1130:1162	determining cell wall composition	1130:1162	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	4	7	theme	IRX9	650:653	arg1	function					638:645	the function	634:645	the function of IRX9, IRX9L, and IRX14	634:671	To investigate the function of IRX9, IRX9L, and IRX14, we identified amino acid residues known to be essential for catalytic function in homologous mammalian proteins and generated modified cDNA clones encoding proteins where these residues would be mutated.
25118690	7	8	theme	homologous	1432:1441	arg1	proteins					1443:1450	homologous proteins	1432:1450	homologous proteins	1432:1450	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	6	9	theme	determining	1130:1140	arg1	composition					1152:1162	determining cell wall composition	1130:1162	determining cell wall composition	1130:1162	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	4	10	theme	IRX9L	656:660	arg1	function					638:645	the function	634:645	the function of IRX9, IRX9L, and IRX14	634:671	To investigate the function of IRX9, IRX9L, and IRX14, we identified amino acid residues known to be essential for catalytic function in homologous mammalian proteins and generated modified cDNA clones encoding proteins where these residues would be mutated.
25118690	6	11	theme	transgenic	1220:1229	arg1	plants					1231:1236	the transgenic plants	1216:1236	the transgenic plants	1216:1236	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	5	12	from	deficient	1000:1008	arg1	synthesis					1019:1027	xylan synthesis	1013:1027	xylan synthesis	1013:1027	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	9	13	theme	substrate-binding	1664:1680	arg1	motif					1686:1690	the substrate-binding DxD motif	1660:1690	the substrate-binding DxD motif	1660:1690	A IRX14 protein mutated in the substrate-binding DxD motif did not complement the irx14 mutant.
25118690	8	14	from	mutant	1587:1592	arg1	acid					1627:1630	the predicted catalytic amino acid	1597:1630	the predicted catalytic amino acid	1597:1630	Two out of three mutated IRX14 complemented the irx14 mutant, including a mutant in the predicted catalytic amino acid.
25118690	10	15	theme	substrate	1735:1743	arg1	binding					1745:1751	substrate binding	1735:1751	substrate binding	1735:1751	Thus, substrate binding is important for IRX14 function but catalytic activity may not be essential for the function of the protein.
25118690	1	16	theme	non-cellulosic	198:211	arg1	polysaccharide					213:226	the main non-cellulosic polysaccharide	189:226	the main non-cellulosic polysaccharide in the secondary cell walls of plants	189:264	Xylans constitute the main non-cellulosic polysaccharide in the secondary cell walls of plants.
25118690	3	17	theme	backbone	584:591	arg1	synthesis					593:601	xylan backbone synthesis	578:601	xylan backbone synthesis in Arabidopsis	578:616	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	1	18	from	polysaccharide	213:226	arg1	walls					250:254	the secondary cell walls	231:254	the secondary cell walls of plants	231:264	Xylans constitute the main non-cellulosic polysaccharide in the secondary cell walls of plants.
25118690	6	19	theme	cross-sections	1198:1211	arg1	analysis					1181:1188	microscopic analysis	1169:1188	microscopic analysis of stem cross-sections of the transgenic plants	1169:1236	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	6	19	theme	cross-sections	1198:1211	arg1	composition					1152:1162	determining cell wall composition	1130:1162	determining cell wall composition	1130:1162	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	6	19	theme	cross-sections	1198:1211	arg1	growth					1122:1127	growth	1122:1127	growth	1122:1127	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	0	20	theme	IRX9	141:144	arg1	function					146:153	IRX9 function	141:153	IRX9 function in Arabidopsis	141:168	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.
25118690	5	21	theme	xylan	1013:1017	arg1	synthesis					1019:1027	xylan synthesis	1013:1027	xylan synthesis	1013:1027	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	11	22	theme	structural	1914:1923	arg1	function					1925:1932	an essential structural function	1901:1932	an essential structural function	1901:1932	The data indicate that IRX9/IRX9L have an essential structural function, most likely by interacting with the IRX10/IRX10L proteins, but do not have an essential catalytic function.
25118690	2	23	theme	Several	267:273	arg1	genes					275:279	Several genes	267:279	Several genes predicted to encode glycosyltransferases	267:320	Several genes predicted to encode glycosyltransferases are required for the synthesis of the xylan backbone even though it is a homopolymer consisting entirely of β-1,4-linked xylose residues.
25118690	7	24	theme	irx9	1325:1328	arg1	phenotype					1337:1345	the irx9 mutant phenotype	1321:1345	the irx9 mutant phenotype	1321:1345	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	4	25	theme	acid	694:697	arg1	residues					699:706	amino acid residues	688:706	amino acid residues known to be essential for catalytic function in homologous mammalian proteins	688:784	To investigate the function of IRX9, IRX9L, and IRX14, we identified amino acid residues known to be essential for catalytic function in homologous mammalian proteins and generated modified cDNA clones encoding proteins where these residues would be mutated.
25118690	6	26	theme	microscopic	1169:1179	arg1	analysis					1181:1188	microscopic analysis	1169:1188	microscopic analysis of stem cross-sections of the transgenic plants	1169:1236	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	0	27	theme	proteins	51:58	arg1	mutagenesis					14:24	Site-directed mutagenesis	0:24	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.	0:169	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.
25118690	10	28	theme	catalytic	1789:1797	arg1	activity					1799:1806	catalytic activity	1789:1806	catalytic activity	1789:1806	Thus, substrate binding is important for IRX14 function but catalytic activity may not be essential for the function of the protein.
25118690	8	29	theme	amino	1621:1625	arg1	acid					1627:1630	the predicted catalytic amino acid	1597:1630	the predicted catalytic amino acid	1597:1630	Two out of three mutated IRX14 complemented the irx14 mutant, including a mutant in the predicted catalytic amino acid.
25118690	12	30	contain	has	2142:2144	arg2	activity					2169:2176	an important enzymatic activity	2146:2176	an important enzymatic activity	2146:2176	Most likely IRX14 also has primarily a structural role, but it cannot be excluded that the protein has an important enzymatic activity.
25118690	12	30	contain	has	2142:2144	arg1	protein					2134:2140	the protein	2130:2140	the protein	2130:2140	Most likely IRX14 also has primarily a structural role, but it cannot be excluded that the protein has an important enzymatic activity.
25118690	5	31	from	synthesis	1019:1027	arg1	mutants					981:987	the irx9 and irx14 mutants	962:987	the irx9 and irx14 mutants	962:987	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	5	31	from	synthesis	1019:1027	arg1	deficient					1000:1008	deficient	1000:1008	deficient	1000:1008	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	4	32	from	function	744:751	arg1	proteins					777:784	homologous mammalian proteins	756:784	homologous mammalian proteins	756:784	To investigate the function of IRX9, IRX9L, and IRX14, we identified amino acid residues known to be essential for catalytic function in homologous mammalian proteins and generated modified cDNA clones encoding proteins where these residues would be mutated.
25118690	5	33	theme	mutated	882:888	arg1	constructs					895:904	The mutated gene constructs	878:904	The mutated gene constructs	878:904	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	3	34	dep	paralogs	525:532	arg1	IRX14L					541:546	IRX14L	541:546	IRX14L	541:546	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	34	dep	paralogs	525:532	arg1	IRX10L					553:558	IRX10L	553:558	IRX10L	553:558	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	34	dep	paralogs	525:532	arg1	IRX9L					534:538	IRX9L	534:538	IRX9L	534:538	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	34	dep	paralogs	525:532	arg1	paralogs					525:532	the paralogs	521:532	the paralogs	521:532	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	11	35	contain	have	1896:1899	arg1	IRX9/IRX9L					1885:1894	IRX9/IRX9L	1885:1894	IRX9/IRX9L	1885:1894	The data indicate that IRX9/IRX9L have an essential structural function, most likely by interacting with the IRX10/IRX10L proteins, but do not have an essential catalytic function.
25118690	11	35	contain	have	1896:1899	arg2	function					1925:1932	an essential structural function	1901:1932	an essential structural function	1901:1932	The data indicate that IRX9/IRX9L have an essential structural function, most likely by interacting with the IRX10/IRX10L proteins, but do not have an essential catalytic function.
25118690	0	36	theme	glycosyltransferase	92:110	arg1	activity					112:119	glycosyltransferase activity	92:119	glycosyltransferase activity	92:119	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.
25118690	5	37	theme	irx9	966:969	arg1	mutants					981:987	the irx9 and irx14 mutants	962:987	the irx9 and irx14 mutants	962:987	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	5	37	theme	irx9	966:969	arg1	deficient					1000:1008	deficient	1000:1008	deficient	1000:1008	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	0	38	theme	Site-directed	0:12	arg1	mutagenesis					14:24	Site-directed mutagenesis	0:24	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.	0:169	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.
25118690	12	39	theme	enzymatic	2159:2167	arg1	activity					2169:2176	an important enzymatic activity	2146:2176	an important enzymatic activity	2146:2176	Most likely IRX14 also has primarily a structural role, but it cannot be excluded that the protein has an important enzymatic activity.
25118690	2	40	theme	β-1,4-linked	430:441	arg1	residues					450:457	β-1,4-linked xylose residues	430:457	β-1,4-linked xylose residues	430:457	Several genes predicted to encode glycosyltransferases are required for the synthesis of the xylan backbone even though it is a homopolymer consisting entirely of β-1,4-linked xylose residues.
25118690	5	41	theme	Arabidopsis	939:949	arg1	plants					951:956	wild-type Arabidopsis plants	929:956	wild-type Arabidopsis plants	929:956	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	7	42	theme	IRX9-L	1286:1291	arg1	able					1302:1305	able	1302:1305	able	1302:1305	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	7	42	theme	IRX9-L	1286:1291	arg1	versions					1265:1272	The six different mutated versions	1239:1272	The six different mutated versions of IRX9 and IRX9-L	1239:1291	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	3	43	theme	putative	464:471	arg1	glycosyltransferases					473:492	The putative glycosyltransferases IRX9, IRX14, and IRX10	460:515	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L)	460:559	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	43	theme	putative	464:471	arg1	IRX14					500:504	IRX14	500:504	IRX14	500:504	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	43	theme	putative	464:471	arg1	IRX10					511:515	IRX10	511:515	IRX10	511:515	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	43	theme	putative	464:471	arg1	IRX9					494:497	IRX9	494:497	IRX9	494:497	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	12	44	theme	structural	2082:2091	arg1	role					2093:2096	a structural role	2080:2096	a structural role	2080:2096	Most likely IRX14 also has primarily a structural role, but it cannot be excluded that the protein has an important enzymatic activity.
25118690	7	45	theme	IRX9	1277:1280	arg1	able					1302:1305	able	1302:1305	able	1302:1305	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	7	45	theme	IRX9	1277:1280	arg1	versions					1265:1272	The six different mutated versions	1239:1272	The six different mutated versions of IRX9 and IRX9-L	1239:1291	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	4	46	theme	cDNA	809:812	arg1	clones					814:819	modified cDNA clones	800:819	modified cDNA clones encoding proteins where these residues would be mutated	800:875	To investigate the function of IRX9, IRX9L, and IRX14, we identified amino acid residues known to be essential for catalytic function in homologous mammalian proteins and generated modified cDNA clones encoding proteins where these residues would be mutated.
25118690	4	47	theme	homologous	756:765	arg1	proteins					777:784	homologous mammalian proteins	756:784	homologous mammalian proteins	756:784	To investigate the function of IRX9, IRX9L, and IRX14, we identified amino acid residues known to be essential for catalytic function in homologous mammalian proteins and generated modified cDNA clones encoding proteins where these residues would be mutated.
25118690	7	48	theme	biological	1478:1487	arg1	function					1489:1496	the biological function	1474:1496	the biological function of IRX9/IRX9L	1474:1510	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	5	49	theme	irx14	975:979	arg1	mutants					981:987	the irx9 and irx14 mutants	962:987	the irx9 and irx14 mutants	962:987	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	5	49	theme	irx14	975:979	arg1	deficient					1000:1008	deficient	1000:1008	deficient	1000:1008	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	7	50	theme	different	1247:1255	arg1	able					1302:1305	able	1302:1305	able	1302:1305	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	7	50	theme	different	1247:1255	arg1	versions					1265:1272	The six different mutated versions	1239:1272	The six different mutated versions of IRX9 and IRX9-L	1239:1291	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	1	51	theme	cell	245:248	arg1	walls					250:254	the secondary cell walls	231:254	the secondary cell walls of plants	231:264	Xylans constitute the main non-cellulosic polysaccharide in the secondary cell walls of plants.
25118690	2	52	theme	backbone	366:373	arg1	synthesis					343:351	the synthesis	339:351	the synthesis of the xylan backbone	339:373	Several genes predicted to encode glycosyltransferases are required for the synthesis of the xylan backbone even though it is a homopolymer consisting entirely of β-1,4-linked xylose residues.
25118690	6	53	theme	proteins	1057:1064	arg1	ability					1034:1040	The ability	1030:1040	The ability of the mutated proteins to complement the mutants	1030:1090	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	11	54	theme	IRX10/IRX10L	1971:1982	arg1	proteins					1984:1991	the IRX10/IRX10L proteins	1967:1991	the IRX10/IRX10L proteins	1967:1991	The data indicate that IRX9/IRX9L have an essential structural function, most likely by interacting with the IRX10/IRX10L proteins, but do not have an essential catalytic function.
25118690	9	55	theme	IRX14	1635:1639	arg1	protein					1641:1647	A IRX14 protein	1633:1647	A IRX14 protein mutated in the substrate-binding DxD motif	1633:1690	A IRX14 protein mutated in the substrate-binding DxD motif did not complement the irx14 mutant.
25118690	8	56	theme	catalytic	1611:1619	arg1	acid					1627:1630	the predicted catalytic amino acid	1597:1630	the predicted catalytic amino acid	1597:1630	Two out of three mutated IRX14 complemented the irx14 mutant, including a mutant in the predicted catalytic amino acid.
25118690	6	57	theme	cell	1142:1145	arg1	composition					1152:1162	determining cell wall composition	1130:1162	determining cell wall composition	1130:1162	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	6	58	theme	plants	1231:1236	arg1	cross-sections					1198:1211	stem cross-sections	1193:1211	stem cross-sections of the transgenic plants	1193:1236	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	1	59	theme	main	193:196	arg1	polysaccharide					213:226	the main non-cellulosic polysaccharide	189:226	the main non-cellulosic polysaccharide in the secondary cell walls of plants	189:264	Xylans constitute the main non-cellulosic polysaccharide in the secondary cell walls of plants.
25118690	9	60	theme	DxD	1682:1684	arg1	motif					1686:1690	the substrate-binding DxD motif	1660:1690	the substrate-binding DxD motif	1660:1690	A IRX14 protein mutated in the substrate-binding DxD motif did not complement the irx14 mutant.
25118690	3	61	theme	xylan	578:582	arg1	synthesis					593:601	xylan backbone synthesis	578:601	xylan backbone synthesis in Arabidopsis	578:616	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	11	62	theme	essential	1904:1912	arg1	function					1925:1932	an essential structural function	1901:1932	an essential structural function	1901:1932	The data indicate that IRX9/IRX9L have an essential structural function, most likely by interacting with the IRX10/IRX10L proteins, but do not have an essential catalytic function.
25118690	5	63	theme	gene	890:893	arg1	constructs					895:904	The mutated gene constructs	878:904	The mutated gene constructs	878:904	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	11	64	theme	essential	2013:2021	arg1	function					2033:2040	an essential catalytic function	2010:2040	an essential catalytic function	2010:2040	The data indicate that IRX9/IRX9L have an essential structural function, most likely by interacting with the IRX10/IRX10L proteins, but do not have an essential catalytic function.
25118690	6	65	theme	stem	1193:1196	arg1	cross-sections					1198:1211	stem cross-sections	1193:1211	stem cross-sections of the transgenic plants	1193:1236	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	5	66	used	used	911:914	arg2	constructs					895:904	The mutated gene constructs	878:904	The mutated gene constructs	878:904	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	9	67	theme	irx14	1715:1719	arg1	mutant					1721:1726	the irx14 mutant	1711:1726	the irx14 mutant	1711:1726	A IRX14 protein mutated in the substrate-binding DxD motif did not complement the irx14 mutant.
25118690	7	68	theme	mutant	1330:1335	arg1	phenotype					1337:1345	the irx9 mutant phenotype	1321:1345	the irx9 mutant phenotype	1321:1345	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	4	69	theme	amino	688:692	arg1	residues					699:706	amino acid residues	688:706	amino acid residues known to be essential for catalytic function in homologous mammalian proteins	688:784	To investigate the function of IRX9, IRX9L, and IRX14, we identified amino acid residues known to be essential for catalytic function in homologous mammalian proteins and generated modified cDNA clones encoding proteins where these residues would be mutated.
25118690	11	70	contain	have	2005:2008	arg1	IRX9/IRX9L					1885:1894	IRX9/IRX9L	1885:1894	IRX9/IRX9L	1885:1894	The data indicate that IRX9/IRX9L have an essential structural function, most likely by interacting with the IRX10/IRX10L proteins, but do not have an essential catalytic function.
25118690	11	70	contain	have	2005:2008	arg2	function					2033:2040	an essential catalytic function	2010:2040	an essential catalytic function	2010:2040	The data indicate that IRX9/IRX9L have an essential structural function, most likely by interacting with the IRX10/IRX10L proteins, but do not have an essential catalytic function.
25118690	0	71	theme	xylan	72:76	arg1	biosynthesis					78:89	xylan biosynthesis	72:89	xylan biosynthesis	72:89	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.
25118690	11	72	theme	catalytic	2023:2031	arg1	function					2033:2040	an essential catalytic function	2010:2040	an essential catalytic function	2010:2040	The data indicate that IRX9/IRX9L have an essential structural function, most likely by interacting with the IRX10/IRX10L proteins, but do not have an essential catalytic function.
25118690	4	73	theme	IRX14	667:671	arg1	function					638:645	the function	634:645	the function of IRX9, IRX9L, and IRX14	634:671	To investigate the function of IRX9, IRX9L, and IRX14, we identified amino acid residues known to be essential for catalytic function in homologous mammalian proteins and generated modified cDNA clones encoding proteins where these residues would be mutated.
25118690	3	74	dep	glycosyltransferases	473:492	arg1	paralogs					525:532	the paralogs	521:532	the paralogs	521:532	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	74	dep	glycosyltransferases	473:492	arg1	IRX10L					553:558	IRX10L	553:558	IRX10L	553:558	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	74	dep	glycosyltransferases	473:492	arg1	IRX14					500:504	IRX14	500:504	IRX14	500:504	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	74	dep	glycosyltransferases	473:492	arg1	IRX9L					534:538	IRX9L	534:538	IRX9L	534:538	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	74	dep	glycosyltransferases	473:492	arg1	IRX10					511:515	IRX10	511:515	IRX10	511:515	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	74	dep	glycosyltransferases	473:492	arg1	IRX14L					541:546	IRX14L	541:546	IRX14L	541:546	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	74	dep	glycosyltransferases	473:492	arg1	glycosyltransferases					473:492	The putative glycosyltransferases IRX9, IRX14, and IRX10	460:515	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L)	460:559	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	3	74	dep	glycosyltransferases	473:492	arg1	IRX9					494:497	IRX9	494:497	IRX9	494:497	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	7	75	from	function	1420:1427	arg1	proteins					1443:1450	homologous proteins	1432:1450	homologous proteins	1432:1450	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	3	76	from	synthesis	593:601	arg1	Arabidopsis					606:616	Arabidopsis	606:616	Arabidopsis	606:616	The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
25118690	6	77	theme	mutated	1049:1055	arg1	proteins					1057:1064	the mutated proteins	1045:1064	the mutated proteins	1045:1064	The ability of the mutated proteins to complement the mutants was investigated by measuring growth, determining cell wall composition, and microscopic analysis of stem cross-sections of the transgenic plants.
25118690	4	78	theme	catalytic	734:742	arg1	function					744:751	catalytic function	734:751	catalytic function in homologous mammalian proteins	734:784	To investigate the function of IRX9, IRX9L, and IRX14, we identified amino acid residues known to be essential for catalytic function in homologous mammalian proteins and generated modified cDNA clones encoding proteins where these residues would be mutated.
25118690	8	79	theme	mutated	1530:1536	arg1	IRX14					1538:1542	Two out of three mutated IRX14	1513:1542	Two out of three mutated IRX14	1513:1542	Two out of three mutated IRX14 complemented the irx14 mutant, including a mutant in the predicted catalytic amino acid.
25118690	0	80	theme	IRX9	29:32	arg1	proteins					51:58	IRX9, IRX9L and IRX14 proteins	29:58	IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis	29:89	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.
25118690	5	81	theme	wild-type	929:937	arg1	plants					951:956	wild-type Arabidopsis plants	929:956	wild-type Arabidopsis plants	929:956	The mutated gene constructs were used to transform wild-type Arabidopsis plants and the irx9 and irx14 mutants, which are deficient in xylan synthesis.
25118690	2	82	theme	xylose	443:448	arg1	residues					450:457	β-1,4-linked xylose residues	430:457	β-1,4-linked xylose residues	430:457	Several genes predicted to encode glycosyltransferases are required for the synthesis of the xylan backbone even though it is a homopolymer consisting entirely of β-1,4-linked xylose residues.
25118690	12	83	theme	important	2149:2157	arg1	activity					2169:2176	an important enzymatic activity	2146:2176	an important enzymatic activity	2146:2176	Most likely IRX14 also has primarily a structural role, but it cannot be excluded that the protein has an important enzymatic activity.
25118690	0	84	theme	IRX9L	35:39	arg1	proteins					51:58	IRX9, IRX9L and IRX14 proteins	29:58	IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis	29:89	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.
25118690	8	85	theme	irx14	1561:1565	arg1	mutant					1567:1572	the irx14 mutant	1557:1572	the irx14 mutant	1557:1572	Two out of three mutated IRX14 complemented the irx14 mutant, including a mutant in the predicted catalytic amino acid.
25118690	0	86	theme	IRX14	45:49	arg1	proteins					51:58	IRX9, IRX9L and IRX14 proteins	29:58	IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis	29:89	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.
25118690	7	87	theme	mutated	1257:1263	arg1	able					1302:1305	able	1302:1305	able	1302:1305	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	7	87	theme	mutated	1257:1263	arg1	versions					1265:1272	The six different mutated versions	1239:1272	The six different mutated versions of IRX9 and IRX9-L	1239:1291	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	10	88	theme	protein	1853:1859	arg1	function					1837:1844	the function	1833:1844	the function of the protein	1833:1859	Thus, substrate binding is important for IRX14 function but catalytic activity may not be essential for the function of the protein.
25118690	4	89	theme	modified	800:807	arg1	clones					814:819	modified cDNA clones	800:819	modified cDNA clones encoding proteins where these residues would be mutated	800:875	To investigate the function of IRX9, IRX9L, and IRX14, we identified amino acid residues known to be essential for catalytic function in homologous mammalian proteins and generated modified cDNA clones encoding proteins where these residues would be mutated.
25118690	7	90	theme	IRX9/IRX9L	1501:1510	arg1	function					1489:1496	the biological function	1474:1496	the biological function of IRX9/IRX9L	1474:1510	The six different mutated versions of IRX9 and IRX9-L were all able to complement the irx9 mutant phenotype, indicating that residues known to be essential for glycosyltransferases function in homologous proteins are not essential for the biological function of IRX9/IRX9L.
25118690	2	91	link	β-1,4-linked	430:441	arg1	residues					450:457	β-1,4-linked xylose residues	430:457	β-1,4-linked xylose residues	430:457	Several genes predicted to encode glycosyltransferases are required for the synthesis of the xylan backbone even though it is a homopolymer consisting entirely of β-1,4-linked xylose residues.
25118690	1	92	theme	secondary	235:243	arg1	walls					250:254	the secondary cell walls	231:254	the secondary cell walls of plants	231:264	Xylans constitute the main non-cellulosic polysaccharide in the secondary cell walls of plants.
25118690	0	93	dep	mutagenesis	14:24	arg1	required					128:135	required	128:135	is not required for IRX9 function in Arabidopsis	121:168	Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.
25118690	4	94	theme	mammalian	767:775	arg1	proteins					777:784	homologous mammalian proteins	756:784	homologous mammalian proteins	756:784	To investigate the function of IRX9, IRX9L, and IRX14, we identified amino acid residues known to be essential for catalytic function in homologous mammalian proteins and generated modified cDNA clones encoding proteins where these residues would be mutated.
28867200	0	0	theme	Flow	76:79	arg1	Rate					81:84	Mass Flow Rate	71:84	Mass Flow Rate	71:84	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.
28867200	5	1	theme	free-fall	816:824	arg1	measurement					831:841	The free-fall flow measurement	812:841	The free-fall flow measurement of the powder and granule blends in a conduit	812:887	The free-fall flow measurement of the powder and granule blends in a conduit is linearly proportional to the X-ray attenuation.
28867200	5	1	theme	free-fall	816:824	arg1	proportional					901:912	proportional	901:912	proportional	901:912	The free-fall flow measurement of the powder and granule blends in a conduit is linearly proportional to the X-ray attenuation.
28867200	2	2	theme	mass	352:355	arg1	rate					362:365	the mass flow rate	348:365	the mass flow rate of the particulates, a critical process variable in continuous manufacturing	348:442	This study evaluates the feasibility of using a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing.
28867200	7	3	from	addition	1194:1201	arg1	composition					1181:1191	composition	1181:1191	composition	1181:1191	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	0	4	theme	Mass	71:74	arg1	Rate					81:84	Mass Flow Rate	71:84	Mass Flow Rate	71:84	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.
28867200	5	5	from	measurement	831:841	arg1	conduit					881:887	a conduit	879:887	a conduit	879:887	The free-fall flow measurement of the powder and granule blends in a conduit is linearly proportional to the X-ray attenuation.
28867200	7	6	theme	operational	1155:1165	arg1	variations					1167:1176	operational variations	1155:1176	operational variations in composition	1155:1191	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	8	7	theme	sensor	1363:1368	arg1	location					1370:1377	the sensor location	1359:1377	the sensor location	1359:1377	The measurement relative standard deviations depend on particulate flow dynamics at the sensor location.
28867200	4	8	theme	blends	592:597	arg1	rates					583:587	Steady-state flow rates	565:587	Steady-state flow rates of blends comprised primarily of acetaminophen and microcrystalline-cellulose	565:665	Steady-state flow rates of blends comprised primarily of acetaminophen and microcrystalline-cellulose are monitored using the sensor, with simultaneous weighing scale measurements, to calibrate the sensor and investigate the measurement accuracy.
28867200	2	9	from	manufacturing	430:442	arg1	variable					407:414	variable	407:414	variable	407:414	This study evaluates the feasibility of using a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing.
28867200	4	10	theme	scale	726:730	arg1	measurements					732:743	simultaneous weighing scale measurements	704:743	simultaneous weighing scale measurements	704:743	Steady-state flow rates of blends comprised primarily of acetaminophen and microcrystalline-cellulose are monitored using the sensor, with simultaneous weighing scale measurements, to calibrate the sensor and investigate the measurement accuracy.
28867200	7	11	theme	material	1240:1247	arg1	reuse					1231:1235	reuse	1231:1235	reuse of material for PAT tool calibration	1231:1272	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	7	11	theme	material	1240:1247	arg1	variations					1167:1176	operational variations	1155:1176	operational variations in composition	1155:1191	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	7	11	theme	material	1240:1247	arg1	addition					1194:1201	addition	1194:1201	addition of lubricant or glidant	1194:1225	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	6	12	theme	measurement	1017:1027	arg1	error					1029:1033	a measurement error	1015:1033	a measurement error of approximately 5%	1015:1053	Relative standard deviations of ∼3%-7% for 1 s monitoring are observed and a measurement error of approximately 5% suggests the usability of the sensor for real-time monitoring.
28867200	0	13	theme	Rate	81:84	arg1	In-line					33:39	In-line	33:39	In-line	33:39	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.
28867200	0	13	theme	Rate	81:84	arg1	Monitoring					57:66	Noninvasive Monitoring	45:66	Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing	45:119	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.
28867200	6	14	theme	standard	949:956	arg1	deviations					958:967	Relative standard deviations	940:967	Relative standard deviations of ∼3%-7% for 1 s monitoring	940:996	Relative standard deviations of ∼3%-7% for 1 s monitoring are observed and a measurement error of approximately 5% suggests the usability of the sensor for real-time monitoring.
28867200	1	15	theme	downstream	149:158	arg1	manufacturing					160:172	continuous downstream manufacturing	138:172	continuous downstream manufacturing of oral solid doses	138:192	The progress in continuous downstream manufacturing of oral solid doses demands effective real-time process management, with monitoring at its core.
28867200	2	16	theme	commercial	319:328	arg1	sensor					330:335	a commercial sensor	317:335	a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing	317:442	This study evaluates the feasibility of using a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing.
28867200	0	17	theme	Tablet	100:105	arg1	Manufacturing					107:119	Continuous Tablet Manufacturing	89:119	Continuous Tablet Manufacturing	89:119	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.
28867200	6	18	theme	Relative	940:947	arg1	deviations					958:967	Relative standard deviations	940:967	Relative standard deviations of ∼3%-7% for 1 s monitoring	940:996	Relative standard deviations of ∼3%-7% for 1 s monitoring are observed and a measurement error of approximately 5% suggests the usability of the sensor for real-time monitoring.
28867200	7	19	theme	sensor	1122:1127	arg1	robust					1144:1149	robust	1144:1149	robust	1144:1149	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	7	19	theme	sensor	1122:1127	arg1	measurement					1129:1139	The sensor measurement	1118:1139	The sensor measurement	1118:1139	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	8	20	theme	relative	1291:1298	arg1	deviations					1309:1318	The measurement relative standard deviations	1275:1318	The measurement relative standard deviations	1275:1318	The measurement relative standard deviations depend on particulate flow dynamics at the sensor location.
28867200	4	21	theme	weighing	717:724	arg1	measurements					732:743	simultaneous weighing scale measurements	704:743	simultaneous weighing scale measurements	704:743	Steady-state flow rates of blends comprised primarily of acetaminophen and microcrystalline-cellulose are monitored using the sensor, with simultaneous weighing scale measurements, to calibrate the sensor and investigate the measurement accuracy.
28867200	0	22	theme	Continuous	89:98	arg1	Manufacturing					107:119	Continuous Tablet Manufacturing	89:119	Continuous Tablet Manufacturing	89:119	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.
28867200	0	23	from	In-line	33:39	arg1	Manufacturing					107:119	Continuous Tablet Manufacturing	89:119	Continuous Tablet Manufacturing	89:119	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.
28867200	8	24	from	location	1370:1377	arg1	dynamics					1347:1354	particulate flow dynamics	1330:1354	particulate flow dynamics at the sensor location	1330:1377	The measurement relative standard deviations depend on particulate flow dynamics at the sensor location.
28867200	3	25	theme	real	554:557	arg1	time					559:562	real time	554:562	real time	554:562	The sensor independently measures X-ray attenuation and cross-correlation velocimetry of particulate flow in real time.
28867200	2	26	theme	particulates	374:385	arg1	rate					362:365	the mass flow rate	348:365	the mass flow rate of the particulates, a critical process variable in continuous manufacturing	348:442	This study evaluates the feasibility of using a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing.
28867200	7	27	theme	lubricant	1206:1214	arg1	reuse					1231:1235	reuse	1231:1235	reuse of material for PAT tool calibration	1231:1272	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	7	27	theme	lubricant	1206:1214	arg1	variations					1167:1176	operational variations	1155:1176	operational variations in composition	1155:1191	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	7	27	theme	lubricant	1206:1214	arg1	addition					1194:1201	addition	1194:1201	addition of lubricant or glidant	1194:1225	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	3	28	theme	flow	546:549	arg1	attenuation					485:495	X-ray attenuation	479:495	X-ray attenuation	479:495	The sensor independently measures X-ray attenuation and cross-correlation velocimetry of particulate flow in real time.
28867200	3	28	theme	flow	546:549	arg1	velocimetry					519:529	cross-correlation velocimetry	501:529	cross-correlation velocimetry	501:529	The sensor independently measures X-ray attenuation and cross-correlation velocimetry of particulate flow in real time.
28867200	1	29	from	progress	126:133	arg1	manufacturing					160:172	continuous downstream manufacturing	138:172	continuous downstream manufacturing of oral solid doses	138:192	The progress in continuous downstream manufacturing of oral solid doses demands effective real-time process management, with monitoring at its core.
28867200	1	30	theme	solid	182:186	arg1	doses					188:192	oral solid doses	177:192	oral solid doses	177:192	The progress in continuous downstream manufacturing of oral solid doses demands effective real-time process management, with monitoring at its core.
28867200	5	31	theme	granule	861:867	arg1	blends					869:874	the powder and granule blends	846:874	blends	869:874	The free-fall flow measurement of the powder and granule blends in a conduit is linearly proportional to the X-ray attenuation.
28867200	4	32	theme	flow	578:581	arg1	rates					583:587	Steady-state flow rates	565:587	Steady-state flow rates of blends comprised primarily of acetaminophen and microcrystalline-cellulose	565:665	Steady-state flow rates of blends comprised primarily of acetaminophen and microcrystalline-cellulose are monitored using the sensor, with simultaneous weighing scale measurements, to calibrate the sensor and investigate the measurement accuracy.
28867200	1	33	theme	doses	188:192	arg1	manufacturing					160:172	continuous downstream manufacturing	138:172	continuous downstream manufacturing of oral solid doses	138:192	The progress in continuous downstream manufacturing of oral solid doses demands effective real-time process management, with monitoring at its core.
28867200	0	34	theme	Sensors	21:27	arg1	Application					0:10	Application	0:10	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.	0:120	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.
28867200	4	35	theme	Steady-state	565:576	arg1	rates					583:587	Steady-state flow rates	565:587	Steady-state flow rates of blends comprised primarily of acetaminophen and microcrystalline-cellulose	565:665	Steady-state flow rates of blends comprised primarily of acetaminophen and microcrystalline-cellulose are monitored using the sensor, with simultaneous weighing scale measurements, to calibrate the sensor and investigate the measurement accuracy.
28867200	8	36	theme	flow	1342:1345	arg1	dynamics					1347:1354	particulate flow dynamics	1330:1354	particulate flow dynamics at the sensor location	1330:1377	The measurement relative standard deviations depend on particulate flow dynamics at the sensor location.
28867200	9	37	theme	particulate	1499:1509	arg1	stream					1511:1516	the particulate stream	1495:1516	the particulate stream	1495:1516	This requires experimental evaluation for a given material at the sensor location, to capture the flow dynamics of the particulate stream through the sensor.
28867200	6	38	theme	real-time	1096:1104	arg1	monitoring					1106:1115	real-time monitoring	1096:1115	real-time monitoring	1096:1115	Relative standard deviations of ∼3%-7% for 1 s monitoring are observed and a measurement error of approximately 5% suggests the usability of the sensor for real-time monitoring.
28867200	2	39	theme	variable	407:414	arg1	particulates					374:385	the particulates	370:385	the particulates	370:385	This study evaluates the feasibility of using a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing.
28867200	2	39	theme	variable	407:414	arg1	process					399:405	a critical process	388:405	a critical process variable in continuous manufacturing	388:442	This study evaluates the feasibility of using a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing.
28867200	0	40	theme	X-Ray	15:19	arg1	Sensors					21:27	X-Ray Sensors	15:27	X-Ray Sensors	15:27	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.
28867200	4	41	theme	measurement	790:800	arg1	accuracy					802:809	the measurement accuracy	786:809	the measurement accuracy	786:809	Steady-state flow rates of blends comprised primarily of acetaminophen and microcrystalline-cellulose are monitored using the sensor, with simultaneous weighing scale measurements, to calibrate the sensor and investigate the measurement accuracy.
28867200	6	42	theme	∼3	972:973	arg1	%					977:977	∼3%-7%	972:977	∼3%-7% for 1 s monitoring	972:996	Relative standard deviations of ∼3%-7% for 1 s monitoring are observed and a measurement error of approximately 5% suggests the usability of the sensor for real-time monitoring.
28867200	1	43	theme	effective	202:210	arg1	management					230:239	effective real-time process management	202:239	effective real-time process management	202:239	The progress in continuous downstream manufacturing of oral solid doses demands effective real-time process management, with monitoring at its core.
28867200	9	44	theme	experimental	1394:1405	arg1	evaluation					1407:1416	experimental evaluation	1394:1416	experimental evaluation for a given material	1394:1437	This requires experimental evaluation for a given material at the sensor location, to capture the flow dynamics of the particulate stream through the sensor.
28867200	7	45	theme	glidant	1219:1225	arg1	reuse					1231:1235	reuse	1231:1235	reuse of material for PAT tool calibration	1231:1272	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	7	45	theme	glidant	1219:1225	arg1	variations					1167:1176	operational variations	1155:1176	operational variations in composition	1155:1191	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	7	45	theme	glidant	1219:1225	arg1	addition					1194:1201	addition	1194:1201	addition of lubricant or glidant	1194:1225	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	2	46	theme	critical	390:397	arg1	particulates					374:385	the particulates	370:385	the particulates	370:385	This study evaluates the feasibility of using a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing.
28867200	2	46	theme	critical	390:397	arg1	process					399:405	a critical process	388:405	a critical process variable in continuous manufacturing	388:442	This study evaluates the feasibility of using a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing.
28867200	4	47	theme	simultaneous	704:715	arg1	measurements					732:743	simultaneous weighing scale measurements	704:743	simultaneous weighing scale measurements	704:743	Steady-state flow rates of blends comprised primarily of acetaminophen and microcrystalline-cellulose are monitored using the sensor, with simultaneous weighing scale measurements, to calibrate the sensor and investigate the measurement accuracy.
28867200	1	48	theme	real-time	212:220	arg1	management					230:239	effective real-time process management	202:239	effective real-time process management	202:239	The progress in continuous downstream manufacturing of oral solid doses demands effective real-time process management, with monitoring at its core.
28867200	6	49	theme	1 s	983:985	arg1	monitoring					987:996	1 s monitoring	983:996	1 s monitoring	983:996	Relative standard deviations of ∼3%-7% for 1 s monitoring are observed and a measurement error of approximately 5% suggests the usability of the sensor for real-time monitoring.
28867200	5	50	theme	flow	826:829	arg1	measurement					831:841	The free-fall flow measurement	812:841	The free-fall flow measurement of the powder and granule blends in a conduit	812:887	The free-fall flow measurement of the powder and granule blends in a conduit is linearly proportional to the X-ray attenuation.
28867200	5	50	theme	flow	826:829	arg1	proportional					901:912	proportional	901:912	proportional	901:912	The free-fall flow measurement of the powder and granule blends in a conduit is linearly proportional to the X-ray attenuation.
28867200	7	51	from	reuse	1231:1235	arg1	composition					1181:1191	composition	1181:1191	composition	1181:1191	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	2	52	theme	continuous	419:428	arg1	manufacturing					430:442	continuous manufacturing	419:442	continuous manufacturing	419:442	This study evaluates the feasibility of using a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing.
28867200	1	53	theme	process	222:228	arg1	management					230:239	effective real-time process management	202:239	effective real-time process management	202:239	The progress in continuous downstream manufacturing of oral solid doses demands effective real-time process management, with monitoring at its core.
28867200	0	54	theme	Noninvasive	45:55	arg1	Monitoring					57:66	Noninvasive Monitoring	45:66	Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing	45:119	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.
28867200	9	55	theme	flow	1478:1481	arg1	dynamics					1483:1490	the flow dynamics	1474:1490	the flow dynamics of the particulate stream	1474:1516	This requires experimental evaluation for a given material at the sensor location, to capture the flow dynamics of the particulate stream through the sensor.
28867200	0	56	from	Monitoring	57:66	arg1	Manufacturing					107:119	Continuous Tablet Manufacturing	89:119	Continuous Tablet Manufacturing	89:119	Application of X-Ray Sensors for In-line and Noninvasive Monitoring of Mass Flow Rate in Continuous Tablet Manufacturing.
28867200	9	57	theme	stream	1511:1516	arg1	dynamics					1483:1490	the flow dynamics	1474:1490	the flow dynamics of the particulate stream	1474:1516	This requires experimental evaluation for a given material at the sensor location, to capture the flow dynamics of the particulate stream through the sensor.
28867200	8	58	theme	particulate	1330:1340	arg1	dynamics					1347:1354	particulate flow dynamics	1330:1354	particulate flow dynamics at the sensor location	1330:1377	The measurement relative standard deviations depend on particulate flow dynamics at the sensor location.
28867200	5	59	theme	powder	850:855	arg1	blends					869:874	the powder and granule blends	846:874	blends	869:874	The free-fall flow measurement of the powder and granule blends in a conduit is linearly proportional to the X-ray attenuation.
28867200	6	60	theme	%	977:977	arg1	deviations					958:967	Relative standard deviations	940:967	Relative standard deviations of ∼3%-7% for 1 s monitoring	940:996	Relative standard deviations of ∼3%-7% for 1 s monitoring are observed and a measurement error of approximately 5% suggests the usability of the sensor for real-time monitoring.
28867200	7	61	from	variations	1167:1176	arg1	composition					1181:1191	composition	1181:1191	composition	1181:1191	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	5	62	theme	blends	869:874	arg1	measurement					831:841	The free-fall flow measurement	812:841	The free-fall flow measurement of the powder and granule blends in a conduit	812:887	The free-fall flow measurement of the powder and granule blends in a conduit is linearly proportional to the X-ray attenuation.
28867200	5	62	theme	blends	869:874	arg1	proportional					901:912	proportional	901:912	proportional	901:912	The free-fall flow measurement of the powder and granule blends in a conduit is linearly proportional to the X-ray attenuation.
28867200	8	63	theme	measurement	1279:1289	arg1	deviations					1309:1318	The measurement relative standard deviations	1275:1318	The measurement relative standard deviations	1275:1318	The measurement relative standard deviations depend on particulate flow dynamics at the sensor location.
28867200	3	64	theme	cross-correlation	501:517	arg1	velocimetry					519:529	cross-correlation velocimetry	501:529	cross-correlation velocimetry	501:529	The sensor independently measures X-ray attenuation and cross-correlation velocimetry of particulate flow in real time.
28867200	9	65	theme	given	1424:1428	arg1	material					1430:1437	a given material	1422:1437	a given material	1422:1437	This requires experimental evaluation for a given material at the sensor location, to capture the flow dynamics of the particulate stream through the sensor.
28867200	3	66	from	measures	470:477	arg1	time					559:562	real time	554:562	real time	554:562	The sensor independently measures X-ray attenuation and cross-correlation velocimetry of particulate flow in real time.
28867200	3	67	theme	X-ray	479:483	arg1	attenuation					485:495	X-ray attenuation	479:495	X-ray attenuation	479:495	The sensor independently measures X-ray attenuation and cross-correlation velocimetry of particulate flow in real time.
28867200	5	68	theme	X-ray	921:925	arg1	attenuation					927:937	the X-ray attenuation	917:937	the X-ray attenuation	917:937	The free-fall flow measurement of the powder and granule blends in a conduit is linearly proportional to the X-ray attenuation.
28867200	6	69	theme	sensor	1085:1090	arg1	usability					1068:1076	the usability	1064:1076	the usability of the sensor for real-time monitoring	1064:1115	Relative standard deviations of ∼3%-7% for 1 s monitoring are observed and a measurement error of approximately 5% suggests the usability of the sensor for real-time monitoring.
28867200	1	70	theme	continuous	138:147	arg1	manufacturing					160:172	continuous downstream manufacturing	138:172	continuous downstream manufacturing of oral solid doses	138:192	The progress in continuous downstream manufacturing of oral solid doses demands effective real-time process management, with monitoring at its core.
28867200	6	71	theme	%	1053:1053	arg1	error					1029:1033	a measurement error	1015:1033	a measurement error of approximately 5%	1015:1053	Relative standard deviations of ∼3%-7% for 1 s monitoring are observed and a measurement error of approximately 5% suggests the usability of the sensor for real-time monitoring.
28867200	7	72	theme	PAT	1253:1255	arg1	calibration					1262:1272	PAT tool calibration	1253:1272	PAT tool calibration	1253:1272	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	8	73	theme	standard	1300:1307	arg1	deviations					1309:1318	The measurement relative standard deviations	1275:1318	The measurement relative standard deviations	1275:1318	The measurement relative standard deviations depend on particulate flow dynamics at the sensor location.
28867200	3	74	theme	particulate	534:544	arg1	flow					546:549	particulate flow	534:549	particulate flow	534:549	The sensor independently measures X-ray attenuation and cross-correlation velocimetry of particulate flow in real time.
28867200	1	75	theme	oral	177:180	arg1	doses					188:192	oral solid doses	177:192	oral solid doses	177:192	The progress in continuous downstream manufacturing of oral solid doses demands effective real-time process management, with monitoring at its core.
28867200	7	76	theme	tool	1257:1260	arg1	calibration					1262:1272	PAT tool calibration	1253:1272	PAT tool calibration	1253:1272	The sensor measurement is robust for operational variations in composition, addition of lubricant or glidant and reuse of material for PAT tool calibration.
28867200	6	77	theme	%	974:974	arg1	%					977:977	∼3%-7%	972:977	∼3%-7% for 1 s monitoring	972:996	Relative standard deviations of ∼3%-7% for 1 s monitoring are observed and a measurement error of approximately 5% suggests the usability of the sensor for real-time monitoring.
28867200	2	78	from	variable	407:414	arg1	manufacturing					430:442	continuous manufacturing	419:442	continuous manufacturing	419:442	This study evaluates the feasibility of using a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing.
28867200	2	79	theme	flow	357:360	arg1	rate					362:365	the mass flow rate	348:365	the mass flow rate of the particulates, a critical process variable in continuous manufacturing	348:442	This study evaluates the feasibility of using a commercial sensor to measure the mass flow rate of the particulates, a critical process variable in continuous manufacturing.
28867200	9	80	theme	sensor	1446:1451	arg1	location					1453:1460	the sensor location	1442:1460	the sensor location	1442:1460	This requires experimental evaluation for a given material at the sensor location, to capture the flow dynamics of the particulate stream through the sensor.
26529192	10	0	theme	scar	1426:1429	arg1	prevention					1431:1440	prominent scar prevention	1416:1440	prominent scar prevention	1416:1440	Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention.
26529192	8	1	theme	excisional	1197:1206	arg1	model					1221:1225	an excisional splint wound model	1194:1225	an excisional splint wound model on C57BL/6J mice	1194:1242	In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice.
26529192	8	2	from	model	1221:1225	arg1	mice					1239:1242	C57BL/6J mice	1230:1242	C57BL/6J mice	1230:1242	In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice.
26529192	1	3	theme	wound	253:257	arg1	devices					264:270	composite wound care devices	243:270	composite wound care devices	243:270	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	8	4	theme	wound	1215:1219	arg1	model					1221:1225	an excisional splint wound model	1194:1225	an excisional splint wound model on C57BL/6J mice	1194:1242	In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice.
26529192	9	5	theme	treated	1257:1263	arg1	wounds					1265:1270	OP-Gel-Aloe treated wounds	1245:1270	OP-Gel-Aloe treated wounds	1245:1270	OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days.
26529192	3	6	theme	aloe	549:552	arg1	vera/curcumin					554:566	aloe vera/curcumin	549:566	aloe vera/curcumin	549:566	A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings.
26529192	12	7	theme	effective	1668:1676	arg1	management					1684:1693	effective wound management	1668:1693	effective wound management	1668:1693	Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.
26529192	2	8	theme	drug	277:280	arg1	characteristics					290:304	The drug release characteristics	273:304	The drug release characteristics of the biocomposite dressings	273:334	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	5	9	theme	fibroblast	870:879	arg1	cells					881:885	NIH3T3 mouse fibroblast cells	857:885	NIH3T3 mouse fibroblast cells	857:885	The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells.
26529192	6	10	theme	cell	996:999	arg1	attachment					1001:1010	good cell attachment	991:1010	good cell attachment	991:1010	OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation.
26529192	2	11	theme	matrix	518:523	arg1	leaching					492:499	leaching	492:499	leaching of the polymeric matrix	492:523	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	9	12	theme	rapid	1287:1291	arg1	healing					1293:1299	very rapid healing	1282:1299	very rapid healing	1282:1299	OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days.
26529192	4	13	theme	antimicrobial	709:721	arg1	activity					723:730	lesser antimicrobial activity	702:730	lesser antimicrobial activity	702:730	However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings.
26529192	12	14	theme	biocomposite	1608:1619	arg1	materials					1654:1662	viable materials	1647:1662	viable materials for effective wound management	1647:1693	Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.
26529192	12	14	theme	biocomposite	1608:1619	arg1	dressings					1621:1629	OP-Gel-Aloe biocomposite dressings	1596:1629	OP-Gel-Aloe biocomposite dressings	1596:1629	Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.
26529192	4	15	theme	Curcumin	673:680	arg1	dressings					682:690	OP-Gel-Aloe Curcumin dressings	661:690	OP-Gel-Aloe Curcumin dressings	661:690	However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings.
26529192	0	16	theme	aloe	53:56	arg1	vera					58:61	aloe vera	53:61	aloe vera	53:61	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.
26529192	3	17	theme	OP-Gel-Aloe	590:600	arg1	dressings					611:619	OP-Gel-Aloe Curcumin dressings	590:619	OP-Gel-Aloe Curcumin dressings	590:619	A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings.
26529192	1	18	theme	oxidized	127:134	arg1	OP-Gel					152:157	OP-Gel	152:157	OP-Gel	152:157	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	1	18	theme	oxidized	127:134	arg1	pectin-gelatin					136:149	oxidized pectin-gelatin	127:149	loaded oxidized pectin-gelatin (OP-Gel) matrices	120:167	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	11	19	theme	nuclei	1577:1582	arg1	migration					1564:1572	migration	1564:1572	migration of nuclei	1564:1582	Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei.
26529192	9	20	theme	healing	1323:1329	arg1	healing					1323:1329	the wound healing	1313:1329	the wound healing	1313:1329	OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days.
26529192	9	20	theme	healing	1323:1329	arg1	%					1308:1308	80%	1306:1308	80% of the wound healing	1306:1329	OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days.
26529192	3	21	used	used	572:575	arg2	composition					534:544	A 50/50 composition	526:544	A 50/50 composition of aloe vera/curcumin	526:566	A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings.
26529192	2	22	dep	released	363:370	arg1	followed					436:443	followed	436:443	followed	436:443	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	11	23	theme	ordered	1485:1491	arg1	formation					1502:1510	an ordered collagen formation	1482:1510	an ordered collagen formation	1482:1510	Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei.
26529192	4	24	theme	OP-Gel-Aloe	744:754	arg1	dressings					776:784	OP-Gel-Aloe and OP-Gel-Curcumin dressings	744:784	OP-Gel-Aloe and OP-Gel-Curcumin dressings	744:784	However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings.
26529192	0	25	theme	Composite	0:8	arg1	dressings					16:24	Composite wound dressings	0:24	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.	0:95	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.
26529192	1	26	theme	care	259:262	arg1	devices					264:270	composite wound care devices	243:270	composite wound care devices	243:270	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	2	27	theme	matrix	428:433	arg1	mechanism					391:399	biphasic mechanism	382:399	biphasic mechanism	382:399	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	4	28	theme	OP-Gel-Curcumin	760:774	arg1	dressings					776:784	OP-Gel-Aloe and OP-Gel-Curcumin dressings	744:784	OP-Gel-Aloe and OP-Gel-Curcumin dressings	744:784	However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings.
26529192	5	29	theme	NIH3T3	857:862	arg1	cells					881:885	NIH3T3 mouse fibroblast cells	857:885	NIH3T3 mouse fibroblast cells	857:885	The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells.
26529192	0	30	theme	pectin	29:34	arg1	dressings					16:24	Composite wound dressings	0:24	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.	0:95	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.
26529192	6	31	theme	treated	900:906	arg1	fibroblasts					908:918	OP-Gel-Aloe treated fibroblasts	888:918	OP-Gel-Aloe treated fibroblasts	888:918	OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation.
26529192	0	32	with	dressings	16:24	arg1	curcumin					67:74	curcumin	67:74	curcumin	67:74	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.
26529192	0	32	with	dressings	16:24	arg1	vera					58:61	aloe vera	53:61	aloe vera	53:61	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.
26529192	5	33	theme	dressings	827:835	arg1	cytocompatibility					791:807	The cytocompatibility	787:807	The cytocompatibility of the fabricated dressings	787:835	The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells.
26529192	2	34	theme	biocomposite	313:324	arg1	dressings					326:334	the biocomposite dressings	309:334	the biocomposite dressings	309:334	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	8	35	theme	healing	1155:1161	arg1	analysis					1163:1170	In vivo wound healing analysis	1141:1170	In vivo wound healing analysis	1141:1170	In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice.
26529192	2	36	dep	mechanism	391:399	arg1	a					380:380	a	380:380	a	380:380	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	10	37	theme	strong	1380:1385	arg1	effect					1405:1410	a strong anti-inflammatory effect	1378:1410	a strong anti-inflammatory effect	1378:1410	Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention.
26529192	1	38	theme	cotton	217:222	arg1	fabrics					224:230	nonwoven cotton fabrics	208:230	nonwoven cotton fabrics	208:230	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	11	39	theme	Histological	1443:1454	arg1	examination					1456:1466	Histological examination	1443:1466	Histological examination	1443:1466	Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei.
26529192	10	40	theme	prominent	1416:1424	arg1	prevention					1431:1440	prominent scar prevention	1416:1440	prominent scar prevention	1416:1440	Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention.
26529192	1	41	theme	composite	243:251	arg1	devices					264:270	composite wound care devices	243:270	composite wound care devices	243:270	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	10	42	theme	aloe	1360:1363	arg1	vera					1365:1368	aloe vera	1360:1368	aloe vera	1360:1368	Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention.
26529192	8	43	theme	splint	1208:1213	arg1	model					1221:1225	an excisional splint wound model	1194:1225	an excisional splint wound model on C57BL/6J mice	1194:1242	In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice.
26529192	6	44	theme	highest	928:934	arg1	viability					936:944	the highest viability	924:944	the highest viability	924:944	OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation.
26529192	7	45	theme	NIH3T3	1127:1132	arg1	cells					1134:1138	NIH3T3 cells	1127:1138	NIH3T3 cells	1127:1138	On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells.
26529192	9	46	theme	OP-Gel-Aloe	1245:1255	arg1	wounds					1265:1270	OP-Gel-Aloe treated wounds	1245:1270	OP-Gel-Aloe treated wounds	1245:1270	OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days.
26529192	12	47	theme	wound	1678:1682	arg1	management					1684:1693	effective wound management	1668:1693	effective wound management	1668:1693	Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.
26529192	0	48	theme	gelatin	40:46	arg1	dressings					16:24	Composite wound dressings	0:24	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.	0:95	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.
26529192	2	49	theme	release	282:288	arg1	characteristics					290:304	The drug release characteristics	273:304	The drug release characteristics of the biocomposite dressings	273:334	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	5	50	theme	mouse	864:868	arg1	cells					881:885	NIH3T3 mouse fibroblast cells	857:885	NIH3T3 mouse fibroblast cells	857:885	The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells.
26529192	8	51	theme	C57BL/6J	1230:1237	arg1	mice					1239:1242	C57BL/6J mice	1230:1242	C57BL/6J mice	1230:1242	In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice.
26529192	8	52	dep	In	1141:1142	arg1	vivo					1144:1147	vivo	1144:1147	vivo	1144:1147	In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice.
26529192	1	53	theme	antimicrobial	182:194	arg1	curcumin					111:118	curcumin	111:118	curcumin	111:118	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	1	53	theme	antimicrobial	182:194	arg1	vera					102:105	Aloe vera	97:105	Aloe vera	97:105	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	1	53	theme	antimicrobial	182:194	arg1	finishes					196:203	antimicrobial finishes	182:203	antimicrobial finishes on nonwoven cotton fabrics	182:230	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	6	54	theme	good	991:994	arg1	attachment					1001:1010	good cell attachment	991:1010	good cell attachment	991:1010	OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation.
26529192	2	55	theme	polymeric	508:516	arg1	matrix					518:523	the polymeric matrix	504:523	the polymeric matrix	504:523	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	3	56	theme	vera/curcumin	554:566	arg1	composition					534:544	A 50/50 composition	526:544	A 50/50 composition of aloe vera/curcumin	526:566	A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings.
26529192	1	57	from	finishes	196:203	arg1	fabrics					224:230	nonwoven cotton fabrics	208:230	nonwoven cotton fabrics	208:230	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	1	58	theme	Aloe	97:100	arg1	curcumin					111:118	curcumin	111:118	curcumin	111:118	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	1	58	theme	Aloe	97:100	arg1	vera					102:105	Aloe vera	97:105	Aloe vera	97:105	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	1	58	theme	Aloe	97:100	arg1	finishes					196:203	antimicrobial finishes	182:203	antimicrobial finishes on nonwoven cotton fabrics	182:230	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	1	59	used	used	174:177	arg2	finishes					196:203	antimicrobial finishes	182:203	antimicrobial finishes on nonwoven cotton fabrics	182:230	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	1	59	used	used	174:177	arg2	curcumin					111:118	curcumin	111:118	curcumin	111:118	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	1	59	used	used	174:177	arg2	vera					102:105	Aloe vera	97:105	Aloe vera	97:105	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	4	60	theme	lesser	702:707	arg1	activity					723:730	lesser antimicrobial activity	702:730	lesser antimicrobial activity	702:730	However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings.
26529192	3	61	theme	Curcumin	602:609	arg1	dressings					611:619	OP-Gel-Aloe Curcumin dressings	590:619	OP-Gel-Aloe Curcumin dressings	590:619	A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings.
26529192	3	62	theme	50/50	528:532	arg1	composition					534:544	A 50/50 composition	526:544	A 50/50 composition of aloe vera/curcumin	526:566	A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings.
26529192	6	63	contain	had	920:922	arg1	fibroblasts					908:918	OP-Gel-Aloe treated fibroblasts	888:918	OP-Gel-Aloe treated fibroblasts	888:918	OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation.
26529192	6	63	contain	had	920:922	arg2	viability					936:944	the highest viability	924:944	the highest viability	924:944	OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation.
26529192	7	64	theme	other	1038:1042	arg1	hand					1044:1047	the other hand	1034:1047	the other hand	1034:1047	On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells.
26529192	1	65	theme	loaded	120:125	arg1	matrices					160:167	loaded oxidized pectin-gelatin (OP-Gel) matrices	120:167	loaded oxidized pectin-gelatin (OP-Gel) matrices	120:167	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	4	66	theme	OP-Gel-Aloe	661:671	arg1	dressings					682:690	OP-Gel-Aloe Curcumin dressings	661:690	OP-Gel-Aloe Curcumin dressings	661:690	However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings.
26529192	9	67	theme	wound	1317:1321	arg1	healing					1323:1329	the wound healing	1313:1329	the wound healing	1313:1329	OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days.
26529192	1	68	theme	pectin-gelatin	136:149	arg1	matrices					160:167	loaded oxidized pectin-gelatin (OP-Gel) matrices	120:167	loaded oxidized pectin-gelatin (OP-Gel) matrices	120:167	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	2	69	theme	aloe	465:468	arg1	vera					470:473	aloe vera	465:473	aloe vera	465:473	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	0	70	theme	bioactive	79:87	arg1	agents					89:94	bioactive agents	79:94	bioactive agents	79:94	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.
26529192	12	71	theme	OP-Gel-Aloe	1596:1606	arg1	materials					1654:1662	viable materials	1647:1662	viable materials for effective wound management	1647:1693	Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.
26529192	12	71	theme	OP-Gel-Aloe	1596:1606	arg1	dressings					1621:1629	OP-Gel-Aloe biocomposite dressings	1596:1629	OP-Gel-Aloe biocomposite dressings	1596:1629	Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.
26529192	1	72	dep	vera	102:105	arg1	matrices					160:167	loaded oxidized pectin-gelatin (OP-Gel) matrices	120:167	loaded oxidized pectin-gelatin (OP-Gel) matrices	120:167	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
26529192	7	73	dep	OP-Gel-Curcumin	1050:1064	arg1	Curcumin					1082:1089	Curcumin	1082:1089	Curcumin	1082:1089	On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells.
26529192	2	74	theme	biphasic	382:389	arg1	mechanism					391:399	biphasic mechanism	382:399	biphasic mechanism	382:399	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	0	75	theme	wound	10:14	arg1	dressings					16:24	Composite wound dressings	0:24	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.	0:95	Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.
26529192	2	76	theme	polymeric	418:426	arg1	matrix					428:433	the polymeric matrix	414:433	the polymeric matrix	414:433	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	11	77	theme	collagen	1493:1500	arg1	formation					1502:1510	an ordered collagen formation	1482:1510	an ordered collagen formation	1482:1510	Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei.
26529192	8	78	theme	In	1141:1142	arg1	healing					1155:1161	In vivo wound healing	1141:1161	In vivo wound healing analysis	1141:1170	In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice.
26529192	5	79	theme	fabricated	816:825	arg1	dressings					827:835	the fabricated dressings	812:835	the fabricated dressings	812:835	The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells.
26529192	12	80	theme	viable	1647:1652	arg1	materials					1654:1662	viable materials	1647:1662	viable materials for effective wound management	1647:1693	Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.
26529192	12	80	theme	viable	1647:1652	arg1	dressings					1621:1629	OP-Gel-Aloe biocomposite dressings	1596:1629	OP-Gel-Aloe biocomposite dressings	1596:1629	Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.
26529192	8	81	theme	wound	1149:1153	arg1	healing					1155:1161	In vivo wound healing	1141:1161	In vivo wound healing analysis	1141:1170	In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice.
26529192	6	82	theme	OP-Gel-Aloe	888:898	arg1	fibroblasts					908:918	OP-Gel-Aloe treated fibroblasts	888:918	OP-Gel-Aloe treated fibroblasts	888:918	OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation.
26529192	2	83	theme	dressings	326:334	arg1	characteristics					290:304	The drug release characteristics	273:304	The drug release characteristics of the biocomposite dressings	273:334	The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix.
26529192	10	84	theme	anti-inflammatory	1387:1403	arg1	effect					1405:1410	a strong anti-inflammatory effect	1378:1410	a strong anti-inflammatory effect	1378:1410	Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention.
26529192	1	85	theme	nonwoven	208:215	arg1	fabrics					224:230	nonwoven cotton fabrics	208:230	nonwoven cotton fabrics	208:230	Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices.
28092950	2	0	theme	heterogeneity	460:472	arg1	area					411:414	the area	407:414	the area of bilayer patterning and phase/composition heterogeneity	407:472	However, some of its potential has yet to be realized, particularly in the area of bilayer patterning and phase/composition heterogeneity.
28092950	1	1	theme	plasma	319:324	arg1	membrane					326:333	the plasma membrane	315:333	the plasma membrane	315:333	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	9	2	theme	partitioning	1666:1677	arg1	studies					1679:1685	function and biomolecule partitioning studies	1641:1685	studies	1679:1685	These combined tools should be helpful to researchers investigating lipid raft dynamics and function and biomolecule partitioning studies.
28092950	8	3	theme	current	1463:1469	arg1	appeal					1471:1476	current appeal	1463:1476	current appeal	1463:1476	Contiguous patterning of supported bilayers as a model of lipid rafts expands the application of the SLB to an area with current appeal and brings with it a useful toolset for characterization and analysis.
28092950	7	4	theme	membrane	1238:1245	arg1	stability					1254:1262	membrane domain stability	1238:1262	membrane domain stability	1238:1262	We examine effectors of membrane domain stability including temperature and cholesterol content to investigate domain dynamics.
28092950	0	5	theme	Polymer	74:80	arg1	Blotting					82:89	Polymer Blotting	74:89	Polymer Blotting	74:89	Two-Phase Contiguous Supported Lipid Bilayer Model for Membrane Rafts via Polymer Blotting and Stenciling.
28092950	1	6	theme	membrane	326:333	arg1	features					303:310	the basic features	293:310	the basic features of the plasma membrane	293:333	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	10	7	theme	patterning	1707:1716	arg1	technique					1718:1726	this patterning technique	1702:1726	this patterning technique	1702:1726	Additionally, this patterning technique may be useful for applications such as bioseparations that exploit differences in lipid phase partitioning or creation of membranes that bind species like viruses preferentially at lipid phase boundaries, to name a few.
28092950	6	8	theme	lipid	1076:1080	arg1	phases					1082:1087	contiguous lipid phases	1065:1087	contiguous lipid phases	1065:1087	In this work, interfaces between contiguous lipid phases define the pattern shapes, with continuity between them allowing transfer of membrane-bound biomolecules between the phases.
28092950	10	9	theme	phase	1915:1919	arg1	boundaries					1921:1930	lipid phase boundaries	1909:1930	lipid phase boundaries	1909:1930	Additionally, this patterning technique may be useful for applications such as bioseparations that exploit differences in lipid phase partitioning or creation of membranes that bind species like viruses preferentially at lipid phase boundaries, to name a few.
28092950	8	10	theme	lipid	1400:1404	arg1	rafts					1406:1410	lipid rafts	1400:1410	lipid rafts	1400:1410	Contiguous patterning of supported bilayers as a model of lipid rafts expands the application of the SLB to an area with current appeal and brings with it a useful toolset for characterization and analysis.
28092950	9	11	theme	function	1641:1648	arg1	studies					1679:1685	function and biomolecule partitioning studies	1641:1685	studies	1679:1685	These combined tools should be helpful to researchers investigating lipid raft dynamics and function and biomolecule partitioning studies.
28092950	3	12	theme	bilayer	512:518	arg1	patterns					520:527	contiguous bilayer patterns	501:527	contiguous bilayer patterns	501:527	In this work, we generate contiguous bilayer patterns as a model system that captures the general features of membrane domains and lipid rafts.
28092950	3	12	theme	bilayer	512:518	arg1	system					540:545	a model system	532:545	a model system that captures the general features of membrane domains and lipid rafts	532:616	In this work, we generate contiguous bilayer patterns as a model system that captures the general features of membrane domains and lipid rafts.
28092950	4	13	theme	polymer	634:640	arg1	templates					642:650	Micropatterned polymer templates	619:650	Micropatterned polymer templates of two types	619:663	Micropatterned polymer templates of two types are investigated for generating patterned bilayer formation: polymer blotting and polymer lift-off stenciling.
28092950	10	14	theme	membranes	1850:1858	arg1	creation					1838:1845	creation	1838:1845	creation of membranes	1838:1858	Additionally, this patterning technique may be useful for applications such as bioseparations that exploit differences in lipid phase partitioning or creation of membranes that bind species like viruses preferentially at lipid phase boundaries, to name a few.
28092950	10	14	theme	membranes	1850:1858	arg1	partitioning					1822:1833	lipid phase partitioning	1810:1833	lipid phase partitioning	1810:1833	Additionally, this patterning technique may be useful for applications such as bioseparations that exploit differences in lipid phase partitioning or creation of membranes that bind species like viruses preferentially at lipid phase boundaries, to name a few.
28092950	8	15	theme	supported	1367:1375	arg1	bilayers					1377:1384	supported bilayers	1367:1384	supported bilayers	1367:1384	Contiguous patterning of supported bilayers as a model of lipid rafts expands the application of the SLB to an area with current appeal and brings with it a useful toolset for characterization and analysis.
28092950	7	16	theme	domain	1247:1252	arg1	stability					1254:1262	membrane domain stability	1238:1262	membrane domain stability	1238:1262	We examine effectors of membrane domain stability including temperature and cholesterol content to investigate domain dynamics.
28092950	6	17	theme	membrane-bound	1166:1179	arg1	biomolecules					1181:1192	membrane-bound biomolecules	1166:1192	membrane-bound biomolecules	1166:1192	In this work, interfaces between contiguous lipid phases define the pattern shapes, with continuity between them allowing transfer of membrane-bound biomolecules between the phases.
28092950	5	18	theme	bilayer	930:936	arg1	diffusion					938:946	bilayer diffusion	930:946	bilayer diffusion	930:946	While these approaches have been used previously to create bilayer arrays by corralling bilayers patches with various types of boundaries impenetrable to bilayer diffusion, unique to the methods presented here, there are no physical barriers to diffusion.
28092950	5	19	theme	boundaries	903:912	arg1	types					894:898	various types	886:898	various types of boundaries impenetrable to bilayer diffusion	886:946	While these approaches have been used previously to create bilayer arrays by corralling bilayers patches with various types of boundaries impenetrable to bilayer diffusion, unique to the methods presented here, there are no physical barriers to diffusion.
28092950	6	20	theme	contiguous	1065:1074	arg1	phases					1082:1087	contiguous lipid phases	1065:1087	contiguous lipid phases	1065:1087	In this work, interfaces between contiguous lipid phases define the pattern shapes, with continuity between them allowing transfer of membrane-bound biomolecules between the phases.
28092950	1	21	theme	useful	159:164	arg1	bilayer					127:133	The supported lipid bilayer	107:133	The supported lipid bilayer	107:133	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	1	21	theme	useful	159:164	arg1	model					166:170	a useful model	157:170	a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane	157:333	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	8	22	theme	Contiguous	1342:1351	arg1	patterning					1353:1362	Contiguous patterning	1342:1362	Contiguous patterning of supported bilayers as a model of lipid rafts	1342:1410	Contiguous patterning of supported bilayers as a model of lipid rafts expands the application of the SLB to an area with current appeal and brings with it a useful toolset for characterization and analysis.
28092950	3	23	theme	contiguous	501:510	arg1	patterns					520:527	contiguous bilayer patterns	501:527	contiguous bilayer patterns	501:527	In this work, we generate contiguous bilayer patterns as a model system that captures the general features of membrane domains and lipid rafts.
28092950	3	23	theme	contiguous	501:510	arg1	system					540:545	a model system	532:545	a model system that captures the general features of membrane domains and lipid rafts	532:616	In this work, we generate contiguous bilayer patterns as a model system that captures the general features of membrane domains and lipid rafts.
28092950	4	24	theme	Micropatterned	619:632	arg1	templates					642:650	Micropatterned polymer templates	619:650	Micropatterned polymer templates of two types	619:663	Micropatterned polymer templates of two types are investigated for generating patterned bilayer formation: polymer blotting and polymer lift-off stenciling.
28092950	2	25	theme	phase/composition	442:458	arg1	heterogeneity					460:472	phase/composition heterogeneity	442:472	phase/composition heterogeneity	442:472	However, some of its potential has yet to be realized, particularly in the area of bilayer patterning and phase/composition heterogeneity.
28092950	1	26	from	appeal	268:273	arg1	learning					278:285	learning	278:285	learning	278:285	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	3	27	theme	model	534:538	arg1	patterns					520:527	contiguous bilayer patterns	501:527	contiguous bilayer patterns	501:527	In this work, we generate contiguous bilayer patterns as a model system that captures the general features of membrane domains and lipid rafts.
28092950	3	27	theme	model	534:538	arg1	system					540:545	a model system	532:545	a model system that captures the general features of membrane domains and lipid rafts	532:616	In this work, we generate contiguous bilayer patterns as a model system that captures the general features of membrane domains and lipid rafts.
28092950	5	28	theme	bilayers	864:871	arg1	patches					873:879	bilayers patches	864:879	bilayers patches	864:879	While these approaches have been used previously to create bilayer arrays by corralling bilayers patches with various types of boundaries impenetrable to bilayer diffusion, unique to the methods presented here, there are no physical barriers to diffusion.
28092950	4	29	theme	polymer	747:753	arg1	stenciling					764:773	polymer lift-off stenciling	747:773	polymer lift-off stenciling	747:773	Micropatterned polymer templates of two types are investigated for generating patterned bilayer formation: polymer blotting and polymer lift-off stenciling.
28092950	10	30	theme	lipid	1909:1913	arg1	boundaries					1921:1930	lipid phase boundaries	1909:1930	lipid phase boundaries	1909:1930	Additionally, this patterning technique may be useful for applications such as bioseparations that exploit differences in lipid phase partitioning or creation of membranes that bind species like viruses preferentially at lipid phase boundaries, to name a few.
28092950	0	31	theme	Lipid	31:35	arg1	Model					45:49	Lipid Bilayer Model	31:49	Lipid Bilayer Model for Membrane Rafts	31:68	Two-Phase Contiguous Supported Lipid Bilayer Model for Membrane Rafts via Polymer Blotting and Stenciling.
28092950	1	32	with	compatible	193:202	arg1	tools					226:230	many biophysical tools	209:230	many biophysical tools	209:230	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	1	32	with	compatible	193:202	arg1	techniques					236:245	techniques	236:245	techniques	236:245	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	7	33	theme	stability	1254:1262	arg1	effectors					1225:1233	effectors	1225:1233	effectors of membrane domain stability including temperature and cholesterol content	1225:1308	We examine effectors of membrane domain stability including temperature and cholesterol content to investigate domain dynamics.
28092950	7	33	theme	stability	1254:1262	arg1	temperature					1274:1284	temperature	1274:1284	temperature	1274:1284	We examine effectors of membrane domain stability including temperature and cholesterol content to investigate domain dynamics.
28092950	7	33	theme	stability	1254:1262	arg1	content					1302:1308	cholesterol content	1290:1308	cholesterol content	1290:1308	We examine effectors of membrane domain stability including temperature and cholesterol content to investigate domain dynamics.
28092950	8	34	theme	rafts	1406:1410	arg1	model					1391:1395	a model	1389:1395	a model of lipid rafts	1389:1410	Contiguous patterning of supported bilayers as a model of lipid rafts expands the application of the SLB to an area with current appeal and brings with it a useful toolset for characterization and analysis.
28092950	1	35	theme	cell	179:182	arg1	membrane					184:191	the cell membrane	175:191	the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane	175:333	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	6	36	theme	pattern	1100:1106	arg1	shapes					1108:1113	the pattern shapes	1096:1113	the pattern shapes	1096:1113	In this work, interfaces between contiguous lipid phases define the pattern shapes, with continuity between them allowing transfer of membrane-bound biomolecules between the phases.
28092950	6	37	theme	biomolecules	1181:1192	arg1	transfer					1154:1161	transfer	1154:1161	transfer of membrane-bound biomolecules between the phases	1154:1211	In this work, interfaces between contiguous lipid phases define the pattern shapes, with continuity between them allowing transfer of membrane-bound biomolecules between the phases.
28092950	3	38	theme	rafts	612:616	arg1	features					573:580	the general features	561:580	the general features of membrane domains and lipid rafts	561:616	In this work, we generate contiguous bilayer patterns as a model system that captures the general features of membrane domains and lipid rafts.
28092950	4	39	theme	polymer	726:732	arg1	blotting					734:741	polymer blotting	726:741	polymer blotting	726:741	Micropatterned polymer templates of two types are investigated for generating patterned bilayer formation: polymer blotting and polymer lift-off stenciling.
28092950	1	40	theme	membrane	184:191	arg1	bilayer					127:133	The supported lipid bilayer	107:133	The supported lipid bilayer	107:133	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	1	40	theme	membrane	184:191	arg1	model					166:170	a useful model	157:170	a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane	157:333	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	5	41	theme	impenetrable	914:925	arg1	boundaries					903:912	boundaries	903:912	boundaries impenetrable to bilayer diffusion	903:946	While these approaches have been used previously to create bilayer arrays by corralling bilayers patches with various types of boundaries impenetrable to bilayer diffusion, unique to the methods presented here, there are no physical barriers to diffusion.
28092950	8	42	with	area	1453:1456	arg1	appeal					1471:1476	current appeal	1463:1476	current appeal	1463:1476	Contiguous patterning of supported bilayers as a model of lipid rafts expands the application of the SLB to an area with current appeal and brings with it a useful toolset for characterization and analysis.
28092950	5	43	theme	bilayer	835:841	arg1	arrays					843:848	bilayer arrays	835:848	bilayer arrays	835:848	While these approaches have been used previously to create bilayer arrays by corralling bilayers patches with various types of boundaries impenetrable to bilayer diffusion, unique to the methods presented here, there are no physical barriers to diffusion.
28092950	5	44	theme	physical	1000:1007	arg1	barriers					1009:1016	no physical barriers	997:1016	no physical barriers to diffusion	997:1029	While these approaches have been used previously to create bilayer arrays by corralling bilayers patches with various types of boundaries impenetrable to bilayer diffusion, unique to the methods presented here, there are no physical barriers to diffusion.
28092950	1	45	theme	compatible	193:202	arg1	membrane					184:191	the cell membrane	175:191	the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane	175:333	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	0	46	theme	Bilayer	37:43	arg1	Model					45:49	Lipid Bilayer Model	31:49	Lipid Bilayer Model for Membrane Rafts	31:68	Two-Phase Contiguous Supported Lipid Bilayer Model for Membrane Rafts via Polymer Blotting and Stenciling.
28092950	2	47	theme	patterning	427:436	arg1	area					411:414	the area	407:414	the area of bilayer patterning and phase/composition heterogeneity	407:472	However, some of its potential has yet to be realized, particularly in the area of bilayer patterning and phase/composition heterogeneity.
28092950	3	48	theme	general	565:571	arg1	features					573:580	the general features	561:580	the general features of membrane domains and lipid rafts	561:616	In this work, we generate contiguous bilayer patterns as a model system that captures the general features of membrane domains and lipid rafts.
28092950	0	49	theme	Membrane	55:62	arg1	Rafts					64:68	Membrane Rafts	55:68	Membrane Rafts	55:68	Two-Phase Contiguous Supported Lipid Bilayer Model for Membrane Rafts via Polymer Blotting and Stenciling.
28092950	1	50	theme	basic	297:301	arg1	features					303:310	the basic features	293:310	the basic features of the plasma membrane	293:333	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	8	51	theme	bilayers	1377:1384	arg1	patterning					1353:1362	Contiguous patterning	1342:1362	Contiguous patterning of supported bilayers as a model of lipid rafts	1342:1410	Contiguous patterning of supported bilayers as a model of lipid rafts expands the application of the SLB to an area with current appeal and brings with it a useful toolset for characterization and analysis.
28092950	3	52	theme	lipid	606:610	arg1	rafts					612:616	lipid rafts	606:616	lipid rafts	606:616	In this work, we generate contiguous bilayer patterns as a model system that captures the general features of membrane domains and lipid rafts.
28092950	9	53	theme	raft	1623:1626	arg1	dynamics					1628:1635	lipid raft dynamics	1617:1635	lipid raft dynamics	1617:1635	These combined tools should be helpful to researchers investigating lipid raft dynamics and function and biomolecule partitioning studies.
28092950	1	54	theme	supported	111:119	arg1	bilayer					127:133	The supported lipid bilayer	107:133	The supported lipid bilayer	107:133	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	1	54	theme	supported	111:119	arg1	model					166:170	a useful model	157:170	a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane	157:333	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	5	55	used	used	809:812	arg2	approaches					788:797	these approaches	782:797	these approaches	782:797	While these approaches have been used previously to create bilayer arrays by corralling bilayers patches with various types of boundaries impenetrable to bilayer diffusion, unique to the methods presented here, there are no physical barriers to diffusion.
28092950	7	56	theme	cholesterol	1290:1300	arg1	content					1302:1308	cholesterol content	1290:1308	cholesterol content	1290:1308	We examine effectors of membrane domain stability including temperature and cholesterol content to investigate domain dynamics.
28092950	1	57	theme	lipid	121:125	arg1	bilayer					127:133	The supported lipid bilayer	107:133	The supported lipid bilayer	107:133	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	1	57	theme	lipid	121:125	arg1	model					166:170	a useful model	157:170	a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane	157:333	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	10	58	theme	lipid	1810:1814	arg1	partitioning					1822:1833	lipid phase partitioning	1810:1833	lipid phase partitioning	1810:1833	Additionally, this patterning technique may be useful for applications such as bioseparations that exploit differences in lipid phase partitioning or creation of membranes that bind species like viruses preferentially at lipid phase boundaries, to name a few.
28092950	8	59	theme	SLB	1443:1445	arg1	application					1424:1434	the application	1420:1434	the application of the SLB to an area with current appeal	1420:1476	Contiguous patterning of supported bilayers as a model of lipid rafts expands the application of the SLB to an area with current appeal and brings with it a useful toolset for characterization and analysis.
28092950	3	60	theme	membrane	585:592	arg1	domains					594:600	membrane domains	585:600	membrane domains	585:600	In this work, we generate contiguous bilayer patterns as a model system that captures the general features of membrane domains and lipid rafts.
28092950	9	61	theme	lipid	1617:1621	arg1	dynamics					1628:1635	lipid raft dynamics	1617:1635	lipid raft dynamics	1617:1635	These combined tools should be helpful to researchers investigating lipid raft dynamics and function and biomolecule partitioning studies.
28092950	4	62	theme	bilayer	707:713	arg1	formation					715:723	patterned bilayer formation	697:723	patterned bilayer formation	697:723	Micropatterned polymer templates of two types are investigated for generating patterned bilayer formation: polymer blotting and polymer lift-off stenciling.
28092950	10	63	theme	phase	1816:1820	arg1	partitioning					1822:1833	lipid phase partitioning	1810:1833	lipid phase partitioning	1810:1833	Additionally, this patterning technique may be useful for applications such as bioseparations that exploit differences in lipid phase partitioning or creation of membranes that bind species like viruses preferentially at lipid phase boundaries, to name a few.
28092950	1	64	theme	many	209:212	arg1	tools					226:230	many biophysical tools	209:230	many biophysical tools	209:230	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	4	65	theme	lift-off	755:762	arg1	stenciling					764:773	polymer lift-off stenciling	747:773	polymer lift-off stenciling	747:773	Micropatterned polymer templates of two types are investigated for generating patterned bilayer formation: polymer blotting and polymer lift-off stenciling.
28092950	3	66	theme	domains	594:600	arg1	features					573:580	the general features	561:580	the general features of membrane domains and lipid rafts	561:616	In this work, we generate contiguous bilayer patterns as a model system that captures the general features of membrane domains and lipid rafts.
28092950	4	67	theme	patterned	697:705	arg1	formation					715:723	patterned bilayer formation	697:723	patterned bilayer formation	697:723	Micropatterned polymer templates of two types are investigated for generating patterned bilayer formation: polymer blotting and polymer lift-off stenciling.
28092950	1	68	theme	biophysical	214:224	arg1	tools					226:230	many biophysical tools	209:230	many biophysical tools	209:230	The supported lipid bilayer has been portrayed as a useful model of the cell membrane compatible with many biophysical tools and techniques that demonstrate its appeal in learning about the basic features of the plasma membrane.
28092950	7	69	theme	domain	1325:1330	arg1	dynamics					1332:1339	domain dynamics	1325:1339	domain dynamics	1325:1339	We examine effectors of membrane domain stability including temperature and cholesterol content to investigate domain dynamics.
28092950	4	70	theme	types	659:663	arg1	templates					642:650	Micropatterned polymer templates	619:650	Micropatterned polymer templates of two types	619:663	Micropatterned polymer templates of two types are investigated for generating patterned bilayer formation: polymer blotting and polymer lift-off stenciling.
28092950	10	71	from	differences	1795:1805	arg1	creation					1838:1845	creation	1838:1845	creation of membranes	1838:1858	Additionally, this patterning technique may be useful for applications such as bioseparations that exploit differences in lipid phase partitioning or creation of membranes that bind species like viruses preferentially at lipid phase boundaries, to name a few.
28092950	10	71	from	differences	1795:1805	arg1	partitioning					1822:1833	lipid phase partitioning	1810:1833	lipid phase partitioning	1810:1833	Additionally, this patterning technique may be useful for applications such as bioseparations that exploit differences in lipid phase partitioning or creation of membranes that bind species like viruses preferentially at lipid phase boundaries, to name a few.
28092950	9	72	theme	combined	1555:1562	arg1	tools					1564:1568	These combined tools	1549:1568	These combined tools	1549:1568	These combined tools should be helpful to researchers investigating lipid raft dynamics and function and biomolecule partitioning studies.
28092950	2	73	theme	bilayer	419:425	arg1	patterning					427:436	bilayer patterning	419:436	bilayer patterning	419:436	However, some of its potential has yet to be realized, particularly in the area of bilayer patterning and phase/composition heterogeneity.
28092950	8	74	theme	useful	1499:1504	arg1	toolset					1506:1512	a useful toolset	1497:1512	a useful toolset for characterization and analysis	1497:1546	Contiguous patterning of supported bilayers as a model of lipid rafts expands the application of the SLB to an area with current appeal and brings with it a useful toolset for characterization and analysis.
28092950	9	75	theme	biomolecule	1654:1664	arg1	studies					1679:1685	function and biomolecule partitioning studies	1641:1685	studies	1679:1685	These combined tools should be helpful to researchers investigating lipid raft dynamics and function and biomolecule partitioning studies.
28092950	5	76	theme	various	886:892	arg1	types					894:898	various types	886:898	various types of boundaries impenetrable to bilayer diffusion	886:946	While these approaches have been used previously to create bilayer arrays by corralling bilayers patches with various types of boundaries impenetrable to bilayer diffusion, unique to the methods presented here, there are no physical barriers to diffusion.
26230911	5	0	from	changes	831:837	arg1	composition					851:861	hydrogel composition	842:861	hydrogel composition	842:861	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	9	1	theme	tissue	1697:1702	arg1	repair					1704:1709	tissue repair	1697:1709	tissue repair	1697:1709	These findings suggest that it may be possible to fine tune capsule breakdown by means of simple changes in material formulation and regulate, and eventually optimize, cell release for tissue repair.
26230911	6	2	theme	microspheres	982:993	arg1	Degradation					953:963	Degradation	953:963	Degradation of pure alginate microspheres	953:993	Degradation of pure alginate microspheres was varied from a few days to several weeks by varying sodium alginate and calcium chloride concentrations.
26230911	2	3	dep	possible	177:184	arg1	deliver					283:289	deliver	283:289	to locally deliver cells that support repair or regeneration processes	272:341	They make it possible to fill tissue defects accurately with an undoubtedly minimally invasive approach and to locally deliver cells that support repair or regeneration processes.
26230911	2	3	dep	possible	177:184	arg1	fill					189:192	fill	189:192	to fill tissue defects accurately with an undoubtedly minimally invasive approach	186:266	They make it possible to fill tissue defects accurately with an undoubtedly minimally invasive approach and to locally deliver cells that support repair or regeneration processes.
26230911	5	4	theme	material	797:804	arg1	stability					806:814	material stability	797:814	material stability resulting from changes in hydrogel composition	797:861	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	3	5	from	diffusion	413:421	arg1	bulk					426:429	bulk	426:429	bulk	426:429	However, their use as a cell carrier is often hindered by inadequate diffusion in bulk.
26230911	6	6	theme	few	1013:1015	arg1	days					1017:1020	a few days	1011:1020	a few days to several weeks	1011:1037	Degradation of pure alginate microspheres was varied from a few days to several weeks by varying sodium alginate and calcium chloride concentrations.
26230911	5	7	from	microcapsules	938:950	arg1	viability					882:890	cell viability	877:890	cell viability after encapsulation	877:910	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	5	7	from	microcapsules	938:950	arg1	release					925:931	in vitro release	916:931	in vitro release from microcapsules	916:950	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	7	8	theme	poloxamer	1119:1127	arg1	addition					1107:1114	The addition	1103:1114	The addition of poloxamer	1103:1127	The addition of poloxamer was also found to accelerate degradation significantly, with capsule breakdown almost complete by two weeks, while chitosan was confirmed to strengthen alginate cross-linking.
26230911	4	9	theme	material	592:599	arg1	thickness					601:609	maximum material thickness	584:609	maximum material thickness	584:609	A possible strategy for overcoming this transport limitation might be represented by injection of rapidly degradable cell-loaded microcapsules, so that maximum material thickness is limited by sphere radius.
26230911	5	10	theme	programmable	675:686	arg1	release					688:694	programmable release	675:694	programmable release of viable cells from alginate-based microcapsules	675:744	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	3	11	theme	cell	368:371	arg1	carrier					373:379	a cell carrier	366:379	a cell carrier	366:379	However, their use as a cell carrier is often hindered by inadequate diffusion in bulk.
26230911	9	12	theme	simple	1602:1607	arg1	changes					1609:1615	simple changes	1602:1615	simple changes in material formulation	1602:1639	These findings suggest that it may be possible to fine tune capsule breakdown by means of simple changes in material formulation and regulate, and eventually optimize, cell release for tissue repair.
26230911	5	13	from	microcapsules	732:744	arg1	cells					706:710	viable cells	699:710	viable cells from alginate-based microcapsules	699:744	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	5	13	from	microcapsules	732:744	arg1	release					688:694	programmable release	675:694	programmable release of viable cells from alginate-based microcapsules	675:744	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	4	14	theme	transport	472:480	arg1	limitation					482:491	this transport limitation	467:491	this transport limitation	467:491	A possible strategy for overcoming this transport limitation might be represented by injection of rapidly degradable cell-loaded microcapsules, so that maximum material thickness is limited by sphere radius.
26230911	8	15	theme	viable	1321:1326	arg1	cells					1328:1332	viable cells	1321:1332	viable cells inside microspheres	1321:1352	The presence of viable cells inside microspheres was revealed after encapsulation, and released cells were observed for all the formulations tested after a time interval dependent on bead degradation speed.
26230911	6	16	theme	several	1025:1031	arg1	weeks					1033:1037	several weeks	1025:1037	several weeks	1025:1037	Degradation of pure alginate microspheres was varied from a few days to several weeks by varying sodium alginate and calcium chloride concentrations.
26230911	5	17	theme	hydrogel	842:849	arg1	composition					851:861	hydrogel composition	842:861	hydrogel composition	842:861	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	8	18	theme	time	1461:1464	arg1	interval					1466:1473	a time interval	1459:1473	a time interval dependent on bead degradation speed	1459:1509	The presence of viable cells inside microspheres was revealed after encapsulation, and released cells were observed for all the formulations tested after a time interval dependent on bead degradation speed.
26230911	0	19	theme	cell	11:14	arg1	delivery					16:23	Programmed cell delivery	0:23	Programmed cell delivery from biodegradable	0:42	Programmed cell delivery from biodegradable microcapsules for tissue repair.
26230911	0	20	from	biodegradable	30:42	arg1	delivery					16:23	Programmed cell delivery	0:23	Programmed cell delivery from biodegradable	0:42	Programmed cell delivery from biodegradable microcapsules for tissue repair.
26230911	9	21	theme	material	1620:1627	arg1	formulation					1629:1639	material formulation	1620:1639	material formulation	1620:1639	These findings suggest that it may be possible to fine tune capsule breakdown by means of simple changes in material formulation and regulate, and eventually optimize, cell release for tissue repair.
26230911	5	22	theme	in	916:917	arg1	release					925:931	in vitro release	916:931	in vitro release from microcapsules	916:950	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	0	23	theme	Programmed	0:9	arg1	delivery					16:23	Programmed cell delivery	0:23	Programmed cell delivery from biodegradable	0:42	Programmed cell delivery from biodegradable microcapsules for tissue repair.
26230911	5	24	from	variations	783:792	arg1	stability					806:814	material stability	797:814	material stability resulting from changes in hydrogel composition	797:861	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	2	25	theme	regeneration	320:331	arg1	processes					333:341	repair or regeneration processes	310:341	repair or regeneration processes	310:341	They make it possible to fill tissue defects accurately with an undoubtedly minimally invasive approach and to locally deliver cells that support repair or regeneration processes.
26230911	5	26	theme	alginate-based	717:730	arg1	microcapsules					732:744	alginate-based microcapsules	717:744	alginate-based microcapsules	717:744	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	8	27	theme	released	1392:1399	arg1	cells					1401:1405	released cells	1392:1405	released cells	1392:1405	The presence of viable cells inside microspheres was revealed after encapsulation, and released cells were observed for all the formulations tested after a time interval dependent on bead degradation speed.
26230911	5	28	dep	in	916:917	arg1	vitro					919:923	vitro	919:923	vitro	919:923	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	8	29	theme	cells	1328:1332	arg1	presence					1309:1316	The presence	1305:1316	The presence of viable cells inside microspheres	1305:1352	The presence of viable cells inside microspheres was revealed after encapsulation, and released cells were observed for all the formulations tested after a time interval dependent on bead degradation speed.
26230911	4	30	theme	maximum	584:590	arg1	thickness					601:609	maximum material thickness	584:609	maximum material thickness	584:609	A possible strategy for overcoming this transport limitation might be represented by injection of rapidly degradable cell-loaded microcapsules, so that maximum material thickness is limited by sphere radius.
26230911	2	31	theme	repair	310:315	arg1	processes					333:341	repair or regeneration processes	310:341	repair or regeneration processes	310:341	They make it possible to fill tissue defects accurately with an undoubtedly minimally invasive approach and to locally deliver cells that support repair or regeneration processes.
26230911	6	32	theme	pure	968:971	arg1	microspheres					982:993	pure alginate microspheres	968:993	pure alginate microspheres	968:993	Degradation of pure alginate microspheres was varied from a few days to several weeks by varying sodium alginate and calcium chloride concentrations.
26230911	5	33	theme	cell	877:880	arg1	viability					882:890	cell viability	877:890	cell viability after encapsulation	877:910	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	2	34	theme	invasive	250:257	arg1	approach					259:266	an undoubtedly minimally invasive approach	225:266	an undoubtedly minimally invasive approach	225:266	They make it possible to fill tissue defects accurately with an undoubtedly minimally invasive approach and to locally deliver cells that support repair or regeneration processes.
26230911	7	35	theme	complete	1215:1222	arg1	breakdown					1198:1206	capsule breakdown	1190:1206	capsule breakdown almost complete	1190:1222	The addition of poloxamer was also found to accelerate degradation significantly, with capsule breakdown almost complete by two weeks, while chitosan was confirmed to strengthen alginate cross-linking.
26230911	4	36	theme	degradable	538:547	arg1	microcapsules					561:573	rapidly degradable cell-loaded microcapsules	530:573	rapidly degradable cell-loaded microcapsules	530:573	A possible strategy for overcoming this transport limitation might be represented by injection of rapidly degradable cell-loaded microcapsules, so that maximum material thickness is limited by sphere radius.
26230911	6	37	theme	chloride	1078:1085	arg1	concentrations					1087:1100	sodium alginate and calcium chloride concentrations	1050:1100	concentrations	1087:1100	Degradation of pure alginate microspheres was varied from a few days to several weeks by varying sodium alginate and calcium chloride concentrations.
26230911	0	38	theme	tissue	62:67	arg1	repair					69:74	tissue repair	62:74	tissue repair	62:74	Programmed cell delivery from biodegradable microcapsules for tissue repair.
26230911	5	39	from	release	688:694	arg1	microcapsules					732:744	alginate-based microcapsules	717:744	alginate-based microcapsules	717:744	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	8	40	theme	dependent	1475:1483	arg1	interval					1466:1473	a time interval	1459:1473	a time interval dependent on bead degradation speed	1459:1509	The presence of viable cells inside microspheres was revealed after encapsulation, and released cells were observed for all the formulations tested after a time interval dependent on bead degradation speed.
26230911	1	41	dep	Injectable	77:86	arg1	hydrogels					103:111	hydrogels	103:111	hydrogels	103:111	Injectable and resorbable hydrogels are an extremely attractive class of biomaterials.
26230911	3	42	theme	inadequate	402:411	arg1	diffusion					413:421	inadequate diffusion	402:421	inadequate diffusion in bulk	402:429	However, their use as a cell carrier is often hindered by inadequate diffusion in bulk.
26230911	4	43	theme	microcapsules	561:573	arg1	injection					517:525	injection	517:525	injection of rapidly degradable cell-loaded microcapsules, so that maximum material thickness is limited by sphere radius	517:637	A possible strategy for overcoming this transport limitation might be represented by injection of rapidly degradable cell-loaded microcapsules, so that maximum material thickness is limited by sphere radius.
26230911	5	44	theme	viable	699:704	arg1	cells					706:710	viable cells	699:710	viable cells from alginate-based microcapsules	699:744	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	1	45	theme	attractive	130:139	arg1	class					141:145	an extremely attractive class	117:145	an extremely attractive class of biomaterials	117:161	Injectable and resorbable hydrogels are an extremely attractive class of biomaterials.
26230911	1	45	theme	attractive	130:139	arg1	Injectable					77:86	Injectable	77:86	Injectable	77:86	Injectable and resorbable hydrogels are an extremely attractive class of biomaterials.
26230911	1	45	theme	attractive	130:139	arg1	resorbable					92:101	resorbable	92:101	resorbable	92:101	Injectable and resorbable hydrogels are an extremely attractive class of biomaterials.
26230911	4	46	theme	cell-loaded	549:559	arg1	microcapsules					561:573	rapidly degradable cell-loaded microcapsules	530:573	rapidly degradable cell-loaded microcapsules	530:573	A possible strategy for overcoming this transport limitation might be represented by injection of rapidly degradable cell-loaded microcapsules, so that maximum material thickness is limited by sphere radius.
26230911	5	47	theme	cells	706:710	arg1	release					688:694	programmable release	675:694	programmable release of viable cells from alginate-based microcapsules	675:744	Here, the possibility of achieving programmable release of viable cells from alginate-based microcapsules was explored in vitro, by evaluating variations in material stability resulting from changes in hydrogel composition and assessing cell viability after encapsulation and in vitro release from microcapsules.
26230911	8	48	theme	bead	1488:1491	arg1	speed					1505:1509	bead degradation speed	1488:1509	bead degradation speed	1488:1509	The presence of viable cells inside microspheres was revealed after encapsulation, and released cells were observed for all the formulations tested after a time interval dependent on bead degradation speed.
26230911	9	49	theme	fine	1562:1565	arg1	breakdown					1580:1588	fine tune capsule breakdown	1562:1588	fine tune capsule breakdown by means of simple changes in material formulation	1562:1639	These findings suggest that it may be possible to fine tune capsule breakdown by means of simple changes in material formulation and regulate, and eventually optimize, cell release for tissue repair.
26230911	4	50	theme	sphere	625:630	arg1	radius					632:637	sphere radius	625:637	sphere radius	625:637	A possible strategy for overcoming this transport limitation might be represented by injection of rapidly degradable cell-loaded microcapsules, so that maximum material thickness is limited by sphere radius.
26230911	9	51	dep	release	1685:1691	arg1	cell					1680:1683	, cell release	1678:1691	cell	1680:1683	These findings suggest that it may be possible to fine tune capsule breakdown by means of simple changes in material formulation and regulate, and eventually optimize, cell release for tissue repair.
26230911	4	52	theme	possible	434:441	arg1	strategy					443:450	A possible strategy	432:450	A possible strategy for overcoming this transport limitation	432:491	A possible strategy for overcoming this transport limitation might be represented by injection of rapidly degradable cell-loaded microcapsules, so that maximum material thickness is limited by sphere radius.
26230911	9	53	theme	tune	1567:1570	arg1	breakdown					1580:1588	fine tune capsule breakdown	1562:1588	fine tune capsule breakdown by means of simple changes in material formulation	1562:1639	These findings suggest that it may be possible to fine tune capsule breakdown by means of simple changes in material formulation and regulate, and eventually optimize, cell release for tissue repair.
26230911	7	54	theme	alginate	1281:1288	arg1	cross-linking					1290:1302	alginate cross-linking	1281:1302	alginate cross-linking	1281:1302	The addition of poloxamer was also found to accelerate degradation significantly, with capsule breakdown almost complete by two weeks, while chitosan was confirmed to strengthen alginate cross-linking.
26230911	2	55	theme	tissue	194:199	arg1	defects					201:207	tissue defects	194:207	tissue defects	194:207	They make it possible to fill tissue defects accurately with an undoubtedly minimally invasive approach and to locally deliver cells that support repair or regeneration processes.
26230911	9	56	theme	capsule	1572:1578	arg1	breakdown					1580:1588	fine tune capsule breakdown	1562:1588	fine tune capsule breakdown by means of simple changes in material formulation	1562:1639	These findings suggest that it may be possible to fine tune capsule breakdown by means of simple changes in material formulation and regulate, and eventually optimize, cell release for tissue repair.
26230911	1	57	theme	biomaterials	150:161	arg1	class					141:145	an extremely attractive class	117:145	an extremely attractive class of biomaterials	117:161	Injectable and resorbable hydrogels are an extremely attractive class of biomaterials.
26230911	1	57	theme	biomaterials	150:161	arg1	Injectable					77:86	Injectable	77:86	Injectable	77:86	Injectable and resorbable hydrogels are an extremely attractive class of biomaterials.
26230911	1	57	theme	biomaterials	150:161	arg1	resorbable					92:101	resorbable	92:101	resorbable	92:101	Injectable and resorbable hydrogels are an extremely attractive class of biomaterials.
26230911	7	58	theme	capsule	1190:1196	arg1	breakdown					1198:1206	capsule breakdown	1190:1206	capsule breakdown almost complete	1190:1222	The addition of poloxamer was also found to accelerate degradation significantly, with capsule breakdown almost complete by two weeks, while chitosan was confirmed to strengthen alginate cross-linking.
26230911	6	59	theme	alginate	973:980	arg1	microspheres					982:993	pure alginate microspheres	968:993	pure alginate microspheres	968:993	Degradation of pure alginate microspheres was varied from a few days to several weeks by varying sodium alginate and calcium chloride concentrations.
26230911	8	60	theme	degradation	1493:1503	arg1	speed					1505:1509	bead degradation speed	1488:1509	bead degradation speed	1488:1509	The presence of viable cells inside microspheres was revealed after encapsulation, and released cells were observed for all the formulations tested after a time interval dependent on bead degradation speed.
26230911	6	61	theme	calcium	1070:1076	arg1	concentrations					1087:1100	sodium alginate and calcium chloride concentrations	1050:1100	concentrations	1087:1100	Degradation of pure alginate microspheres was varied from a few days to several weeks by varying sodium alginate and calcium chloride concentrations.
26230911	9	62	from	changes	1609:1615	arg1	formulation					1629:1639	material formulation	1620:1639	material formulation	1620:1639	These findings suggest that it may be possible to fine tune capsule breakdown by means of simple changes in material formulation and regulate, and eventually optimize, cell release for tissue repair.
26230911	6	63	theme	sodium	1050:1055	arg1	alginate					1057:1064	sodium alginate and calcium chloride concentrations	1050:1100	alginate	1057:1064	Degradation of pure alginate microspheres was varied from a few days to several weeks by varying sodium alginate and calcium chloride concentrations.
28064056	0	0	theme	bone	128:131	arg1	engineering					140:150	bone tissue engineering	128:150	bone tissue engineering	128:150	Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate scaffolds for bone tissue engineering.
28064056	4	1	theme	mechanical	934:943	arg1	properties					945:954	mechanical properties	934:954	mechanical properties	934:954	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	8	2	theme	prepared	1416:1423	arg1	scaffolds					1425:1433	the prepared scaffolds	1412:1433	the prepared scaffolds	1412:1433	Additionally, Preliminary cell electrical tests proved the efficiency of the prepared scaffolds for cell electrical applications.
28064056	2	3	theme	bone	401:404	arg1	tissue					406:411	the bone tissue	397:411	the bone tissue	397:411	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	4	4	from	decrease	922:929	arg1	properties					945:954	mechanical properties	934:954	mechanical properties	934:954	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	4	4	from	decrease	922:929	arg1	content					986:992	1w/v% content	980:992	1w/v% content of f-MWCNTs	980:1004	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	5	5	theme	cytotoxicity	1017:1028	arg1	assay					1030:1034	MTT cytotoxicity assay	1013:1034	MTT cytotoxicity assay	1013:1034	Also, MTT cytotoxicity assay was conducted for the scaffolds and no significant cytotoxicity was observed.
28064056	6	6	theme	Phosphatase	1164:1174	arg1	activity					1176:1183	higher alkaline Phosphatase activity	1148:1183	higher alkaline Phosphatase activity	1148:1183	Increasing f-MWCNT content led to higher alkaline Phosphatase activity.
28064056	7	7	theme	overall	1190:1196	arg1	results					1198:1204	The overall results	1186:1204	The overall results	1186:1204	The overall results show that composites with f-MWCNT content between 0.1w/v% and 0.5w/v% are the most suitable for bone tissue engineering application.
28064056	4	8	theme	mechanical	841:850	arg1	properties					852:861	electrical conductivity and mechanical properties	813:861	properties	852:861	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	4	9	theme	electrical	813:822	arg1	conductivity					824:835	electrical conductivity and mechanical properties	813:861	conductivity	824:835	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	0	10	theme	tissue	133:138	arg1	engineering					140:150	bone tissue engineering	128:150	bone tissue engineering	128:150	Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate scaffolds for bone tissue engineering.
28064056	2	11	from	component	488:496	arg1	years					508:512	recent years	501:512	recent years	501:512	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	3	12	theme	functionalized	570:583	arg1	f-MWCNT					615:621	f-MWCNT	615:621	f-MWCNT	615:621	In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized.
28064056	3	12	theme	functionalized	570:583	arg1	Nanotubes					604:612	functionalized Multiwalled Carbon Nanotubes	570:612	functionalized Multiwalled Carbon Nanotubes (f-MWCNT)	570:622	In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized.
28064056	7	13	theme	engineering	1314:1324	arg1	application					1326:1336	bone tissue engineering application	1302:1336	bone tissue engineering application	1302:1336	The overall results show that composites with f-MWCNT content between 0.1w/v% and 0.5w/v% are the most suitable for bone tissue engineering application.
28064056	2	14	theme	mechanical	328:337	arg1	strength					339:346	high mechanical strength	323:346	high mechanical strength	323:346	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	4	15	theme	f-MWCNT	785:791	arg1	content					793:799	increasing f-MWCNT content	774:799	increasing f-MWCNT content	774:799	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	4	16	theme	increasing	774:783	arg1	content					793:799	increasing f-MWCNT content	774:799	increasing f-MWCNT content	774:799	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	6	17	theme	f-MWCNT	1125:1131	arg1	content					1133:1139	Increasing f-MWCNT content	1114:1139	Increasing f-MWCNT content	1114:1139	Increasing f-MWCNT content led to higher alkaline Phosphatase activity.
28064056	2	18	theme	high	323:326	arg1	strength					339:346	high mechanical strength	323:346	high mechanical strength	323:346	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	6	19	theme	alkaline	1155:1162	arg1	Phosphatase					1164:1174	higher alkaline Phosphatase	1148:1174	higher alkaline Phosphatase activity	1148:1183	Increasing f-MWCNT content led to higher alkaline Phosphatase activity.
28064056	2	20	theme	scaffold	479:486	arg1	component					488:496	a bone tissue scaffold component	465:496	a bone tissue scaffold component in recent years	465:512	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	2	20	theme	scaffold	479:486	arg1	attention					452:460	attention	452:460	attention	452:460	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	2	20	theme	scaffold	479:486	arg1	lot					445:447	a lot	443:447	a lot of attention	443:460	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	2	21	theme	recent	501:506	arg1	years					508:512	recent years	501:512	recent years	501:512	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	8	22	theme	cell	1439:1442	arg1	applications					1455:1466	cell electrical applications	1439:1466	cell electrical applications	1439:1466	Additionally, Preliminary cell electrical tests proved the efficiency of the prepared scaffolds for cell electrical applications.
28064056	6	23	theme	higher	1148:1153	arg1	Phosphatase					1164:1174	higher alkaline Phosphatase	1148:1174	higher alkaline Phosphatase activity	1148:1183	Increasing f-MWCNT content led to higher alkaline Phosphatase activity.
28064056	2	24	theme	tissue	472:477	arg1	component					488:496	a bone tissue scaffold component	465:496	a bone tissue scaffold component in recent years	465:512	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	2	24	theme	tissue	472:477	arg1	attention					452:460	attention	452:460	attention	452:460	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	2	24	theme	tissue	472:477	arg1	lot					445:447	a lot	443:447	a lot of attention	443:460	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	3	25	theme	composite	537:545	arg1	scaffolds					547:555	composite scaffolds	537:555	composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate	537:679	In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized.
28064056	2	26	theme	bone	467:470	arg1	component					488:496	a bone tissue scaffold component	465:496	a bone tissue scaffold component in recent years	465:512	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	2	26	theme	bone	467:470	arg1	attention					452:460	attention	452:460	attention	452:460	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	2	26	theme	bone	467:470	arg1	lot					445:447	a lot	443:447	a lot of attention	443:460	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	7	27	theme	f-MWCNT	1232:1238	arg1	content					1240:1246	f-MWCNT content	1232:1246	f-MWCNT content	1232:1246	The overall results show that composites with f-MWCNT content between 0.1w/v% and 0.5w/v% are the most suitable for bone tissue engineering application.
28064056	2	28	theme	carbon	414:419	arg1	nanotubes					421:429	carbon nanotubes	414:429	carbon nanotubes	414:429	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	2	29	theme	tissue	406:411	arg1	characteristics					378:392	the most important characteristics	359:392	the most important characteristics of the bone tissue	359:411	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	1	30	from	limitation	161:170	arg1	scaffolds					202:210	current tissue engineering scaffolds	175:210	current tissue engineering scaffolds	175:210	A major limitation in current tissue engineering scaffolds is that some of the most important characteristics of the intended tissue are ignored.
28064056	3	31	theme	Multiwalled	585:595	arg1	f-MWCNT					615:621	f-MWCNT	615:621	f-MWCNT	615:621	In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized.
28064056	3	31	theme	Multiwalled	585:595	arg1	Nanotubes					604:612	functionalized Multiwalled Carbon Nanotubes	570:612	functionalized Multiwalled Carbon Nanotubes (f-MWCNT)	570:622	In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized.
28064056	2	32	theme	attention	452:460	arg1	component					488:496	a bone tissue scaffold component	465:496	a bone tissue scaffold component in recent years	465:512	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	2	32	theme	attention	452:460	arg1	lot					445:447	a lot	443:447	a lot of attention	443:460	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	2	32	theme	attention	452:460	arg1	attention					452:460	attention	452:460	attention	452:460	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	4	33	dep	such	912:915	arg1	as					917:918	as	917:918	as	917:918	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	8	34	theme	electrical	1444:1453	arg1	applications					1455:1466	cell electrical applications	1439:1466	cell electrical applications	1439:1466	Additionally, Preliminary cell electrical tests proved the efficiency of the prepared scaffolds for cell electrical applications.
28064056	3	35	theme	Carbon	597:602	arg1	f-MWCNT					615:621	f-MWCNT	615:621	f-MWCNT	615:621	In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized.
28064056	3	35	theme	Carbon	597:602	arg1	Nanotubes					604:612	functionalized Multiwalled Carbon Nanotubes	570:612	functionalized Multiwalled Carbon Nanotubes (f-MWCNT)	570:622	In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized.
28064056	1	36	theme	important	237:245	arg1	characteristics					247:261	the most important characteristics	228:261	the most important characteristics of the intended tissue	228:284	A major limitation in current tissue engineering scaffolds is that some of the most important characteristics of the intended tissue are ignored.
28064056	0	37	theme	functionalized	42:55	arg1	nanotubes/chitosan/β-Glycerophosphate					76:112	novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate	36:112	novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate	36:112	Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate scaffolds for bone tissue engineering.
28064056	8	38	theme	electrical	1370:1379	arg1	tests					1381:1385	Preliminary cell electrical tests	1353:1385	Preliminary cell electrical tests	1353:1385	Additionally, Preliminary cell electrical tests proved the efficiency of the prepared scaffolds for cell electrical applications.
28064056	8	39	theme	cell	1365:1368	arg1	tests					1381:1385	Preliminary cell electrical tests	1353:1385	Preliminary cell electrical tests	1353:1385	Additionally, Preliminary cell electrical tests proved the efficiency of the prepared scaffolds for cell electrical applications.
28064056	5	40	theme	significant	1075:1085	arg1	cytotoxicity					1087:1098	no significant cytotoxicity	1072:1098	no significant cytotoxicity	1072:1098	Also, MTT cytotoxicity assay was conducted for the scaffolds and no significant cytotoxicity was observed.
28064056	6	41	theme	Increasing	1114:1123	arg1	content					1133:1139	Increasing f-MWCNT content	1114:1139	Increasing f-MWCNT content	1114:1139	Increasing f-MWCNT content led to higher alkaline Phosphatase activity.
28064056	0	42	theme	novel	36:40	arg1	nanotubes/chitosan/β-Glycerophosphate					76:112	novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate	36:112	novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate	36:112	Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate scaffolds for bone tissue engineering.
28064056	4	43	theme	f-MWCNTs	997:1004	arg1	content					986:992	1w/v% content	980:992	1w/v% content of f-MWCNTs	980:1004	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	3	44	theme	medium	625:630	arg1	weight					642:647	medium molecular weight	625:647	medium molecular weight chitosan	625:656	In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized.
28064056	8	45	theme	Preliminary	1353:1363	arg1	tests					1381:1385	Preliminary cell electrical tests	1353:1385	Preliminary cell electrical tests	1353:1385	Additionally, Preliminary cell electrical tests proved the efficiency of the prepared scaffolds for cell electrical applications.
28064056	0	46	theme	carbon	69:74	arg1	nanotubes/chitosan/β-Glycerophosphate					76:112	novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate	36:112	novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate	36:112	Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate scaffolds for bone tissue engineering.
28064056	3	47	theme	present	522:528	arg1	study					530:534	the present study	518:534	the present study	518:534	In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized.
28064056	7	48	theme	bone	1302:1305	arg1	application					1326:1336	bone tissue engineering application	1302:1336	bone tissue engineering application	1302:1336	The overall results show that composites with f-MWCNT content between 0.1w/v% and 0.5w/v% are the most suitable for bone tissue engineering application.
28064056	1	49	theme	major	155:159	arg1	limitation					161:170	A major limitation	153:170	A major limitation in current tissue engineering scaffolds	153:210	A major limitation in current tissue engineering scaffolds is that some of the most important characteristics of the intended tissue are ignored.
28064056	0	50	theme	multiwalled	57:67	arg1	nanotubes/chitosan/β-Glycerophosphate					76:112	novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate	36:112	novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate	36:112	Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate scaffolds for bone tissue engineering.
28064056	2	51	theme	characteristics	378:392	arg1	characteristics					378:392	the most important characteristics	359:392	the most important characteristics of the bone tissue	359:411	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	2	51	theme	characteristics	378:392	arg1	two					352:354	two	352:354	two	352:354	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	4	52	theme	%	984:984	arg1	content					986:992	1w/v% content	980:992	1w/v% content of f-MWCNTs	980:1004	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	4	53	theme	mechanical	737:746	arg1	tests					748:752	mechanical tests	737:752	mechanical tests	737:752	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	2	54	theme	important	368:376	arg1	characteristics					378:392	the most important characteristics	359:392	the most important characteristics of the bone tissue	359:411	As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years.
28064056	5	55	theme	MTT	1013:1015	arg1	assay					1030:1034	MTT cytotoxicity assay	1013:1034	MTT cytotoxicity assay	1013:1034	Also, MTT cytotoxicity assay was conducted for the scaffolds and no significant cytotoxicity was observed.
28064056	4	56	theme	1w/v	980:983	arg1	%					984:984	%	984:984	%	984:984	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	7	57	with	composites	1216:1225	arg1	content					1240:1246	f-MWCNT content	1232:1246	f-MWCNT content	1232:1246	The overall results show that composites with f-MWCNT content between 0.1w/v% and 0.5w/v% are the most suitable for bone tissue engineering application.
28064056	1	58	theme	current	175:181	arg1	scaffolds					202:210	current tissue engineering scaffolds	175:210	current tissue engineering scaffolds	175:210	A major limitation in current tissue engineering scaffolds is that some of the most important characteristics of the intended tissue are ignored.
28064056	8	59	theme	scaffolds	1425:1433	arg1	efficiency					1398:1407	the efficiency	1394:1407	the efficiency of the prepared scaffolds for cell electrical applications	1394:1466	Additionally, Preliminary cell electrical tests proved the efficiency of the prepared scaffolds for cell electrical applications.
28064056	1	60	theme	intended	270:277	arg1	tissue					279:284	the intended tissue	266:284	the intended tissue	266:284	A major limitation in current tissue engineering scaffolds is that some of the most important characteristics of the intended tissue are ignored.
28064056	4	61	from	biodegradability	960:975	arg1	properties					945:954	mechanical properties	934:954	mechanical properties	934:954	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	4	61	from	biodegradability	960:975	arg1	content					986:992	1w/v% content	980:992	1w/v% content of f-MWCNTs	980:1004	Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs.
28064056	3	62	theme	molecular	632:640	arg1	weight					642:647	medium molecular weight	625:647	medium molecular weight chitosan	625:656	In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized.
28064056	1	63	theme	tissue	183:188	arg1	scaffolds					202:210	current tissue engineering scaffolds	175:210	current tissue engineering scaffolds	175:210	A major limitation in current tissue engineering scaffolds is that some of the most important characteristics of the intended tissue are ignored.
28064056	1	64	theme	tissue	279:284	arg1	characteristics					247:261	the most important characteristics	228:261	the most important characteristics of the intended tissue	228:284	A major limitation in current tissue engineering scaffolds is that some of the most important characteristics of the intended tissue are ignored.
28064056	0	65	theme	nanotubes/chitosan/β-Glycerophosphate	76:112	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate scaffolds for bone tissue engineering.
28064056	0	65	theme	nanotubes/chitosan/β-Glycerophosphate	76:112	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate scaffolds for bone tissue engineering.
28064056	3	66	theme	weight	642:647	arg1	chitosan					649:656	medium molecular weight chitosan	625:656	medium molecular weight chitosan	625:656	In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized.
28064056	1	67	theme	engineering	190:200	arg1	scaffolds					202:210	current tissue engineering scaffolds	175:210	current tissue engineering scaffolds	175:210	A major limitation in current tissue engineering scaffolds is that some of the most important characteristics of the intended tissue are ignored.
28064056	7	68	theme	tissue	1307:1312	arg1	application					1326:1336	bone tissue engineering application	1302:1336	bone tissue engineering application	1302:1336	The overall results show that composites with f-MWCNT content between 0.1w/v% and 0.5w/v% are the most suitable for bone tissue engineering application.
27987890	0	0	theme	acid	109:112	arg1	delivery					89:96	simultaneous delivery	76:96	simultaneous delivery of ferulic acid and resveratrol	76:128	Fabrication of chitosan-polycaprolactone composite nanofibrous scaffold for simultaneous delivery of ferulic acid and resveratrol.
27987890	2	1	theme	simultaneous	411:422	arg1	delivery					429:436	efficient simultaneous drug delivery	401:436	efficient simultaneous drug delivery	401:436	This study deals with the designing and development of coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings for efficient simultaneous drug delivery.
27987890	7	2	theme	complete	1010:1017	arg1	healing					1019:1025	complete healing	1010:1025	complete healing in 15days	1010:1035	The scaffold treated rats exhibited complete healing in 15days in comparison to the controls that healed in 20days.
27987890	0	3	theme	ferulic	101:107	arg1	acid					109:112	ferulic acid	101:112	ferulic acid	101:112	Fabrication of chitosan-polycaprolactone composite nanofibrous scaffold for simultaneous delivery of ferulic acid and resveratrol.
27987890	6	4	theme	in	935:936	arg1	biocompatibility					956:971	good in vitro and in vivo biocompatibility	930:971	good in vitro and in vivo biocompatibility	930:971	Functional characterization of the nanofibrous wound dressing exhibited good in vitro and in vivo biocompatibility.
27987890	1	5	theme	various	185:191	arg1	mechanisms					193:202	various mechanisms	185:202	various mechanisms making it intricate for designing a scaffold for therapeutic use	185:267	Wound healing is a complex cellular process involving various mechanisms making it intricate for designing a scaffold for therapeutic use.
27987890	7	6	theme	treated	987:993	arg1	rats					995:998	The scaffold treated rats	974:998	The scaffold treated rats	974:998	The scaffold treated rats exhibited complete healing in 15days in comparison to the controls that healed in 20days.
27987890	0	7	theme	resveratrol	118:128	arg1	delivery					89:96	simultaneous delivery	76:96	simultaneous delivery of ferulic acid and resveratrol	76:128	Fabrication of chitosan-polycaprolactone composite nanofibrous scaffold for simultaneous delivery of ferulic acid and resveratrol.
27987890	4	8	theme	Structural	609:618	arg1	characterization					641:656	Structural and physico-chemical characterization	609:656	Structural and physico-chemical characterization of the scaffold	609:672	Structural and physico-chemical characterization of the scaffold confirmed the encapsulation of both the molecules.
27987890	2	9	theme	efficient	401:409	arg1	delivery					429:436	efficient simultaneous drug delivery	401:436	efficient simultaneous drug delivery	401:436	This study deals with the designing and development of coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings for efficient simultaneous drug delivery.
27987890	6	10	theme	good	930:933	arg1	biocompatibility					956:971	good in vitro and in vivo biocompatibility	930:971	good in vitro and in vivo biocompatibility	930:971	Functional characterization of the nanofibrous wound dressing exhibited good in vitro and in vivo biocompatibility.
27987890	7	11	dep	controls	1058:1065	arg1	comparison					1040:1049	comparison	1040:1049	comparison	1040:1049	The scaffold treated rats exhibited complete healing in 15days in comparison to the controls that healed in 20days.
27987890	4	12	theme	molecules	714:722	arg1	encapsulation					688:700	the encapsulation	684:700	the encapsulation of both the molecules	684:722	Structural and physico-chemical characterization of the scaffold confirmed the encapsulation of both the molecules.
27987890	7	13	from	healing	1019:1025	arg1	15days					1030:1035	15days	1030:1035	15days	1030:1035	The scaffold treated rats exhibited complete healing in 15days in comparison to the controls that healed in 20days.
27987890	5	14	theme	resveratrol	787:797	arg1	release					771:777	sustained release	761:777	sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h	761:855	The in vitro release studies showed sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h.
27987890	5	15	theme	in	729:730	arg1	studies					746:752	The in vitro release studies	725:752	The in vitro release studies	725:752	The in vitro release studies showed sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h.
27987890	2	16	theme	nanofiber	371:379	arg1	dressings					387:395	coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings	325:395	coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings	325:395	This study deals with the designing and development of coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings for efficient simultaneous drug delivery.
27987890	5	17	theme	release	738:744	arg1	studies					746:752	The in vitro release studies	725:752	The in vitro release studies	725:752	The in vitro release studies showed sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h.
27987890	6	18	theme	dressing	911:918	arg1	characterization					869:884	Functional characterization	858:884	Functional characterization of the nanofibrous wound dressing	858:918	Functional characterization of the nanofibrous wound dressing exhibited good in vitro and in vivo biocompatibility.
27987890	7	19	theme	scaffold	978:985	arg1	rats					995:998	The scaffold treated rats	974:998	The scaffold treated rats	974:998	The scaffold treated rats exhibited complete healing in 15days in comparison to the controls that healed in 20days.
27987890	3	20	theme	core-shell	503:512	arg1	nanofibers					514:523	core-shell nanofibers	503:523	core-shell nanofibers	503:523	Ferulic acid and resveratrol were chosen for incorporation into core-shell nanofibers for their efficacy in anti-inflammatory and pro-angiogenic activities respectively.
27987890	5	21	theme	till	847:850	arg1	120h					852:855	till 120h	847:855	till 120h	847:855	The in vitro release studies showed sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h.
27987890	8	22	theme	sustained	1156:1164	arg1	systems					1180:1186	sequential and sustained drug delivery systems	1141:1186	sequential and sustained drug delivery systems	1141:1186	The study therefore, opens up venues for designing sequential and sustained drug delivery systems in wound therapeutics.
27987890	6	23	theme	wound	905:909	arg1	dressing					911:918	the nanofibrous wound dressing	889:918	the nanofibrous wound dressing	889:918	Functional characterization of the nanofibrous wound dressing exhibited good in vitro and in vivo biocompatibility.
27987890	8	24	theme	sequential	1141:1150	arg1	systems					1180:1186	sequential and sustained drug delivery systems	1141:1186	sequential and sustained drug delivery systems	1141:1186	The study therefore, opens up venues for designing sequential and sustained drug delivery systems in wound therapeutics.
27987890	6	25	theme	nanofibrous	893:903	arg1	dressing					911:918	the nanofibrous wound dressing	889:918	the nanofibrous wound dressing	889:918	Functional characterization of the nanofibrous wound dressing exhibited good in vitro and in vivo biocompatibility.
27987890	2	26	theme	Chitosan-Polycaprolactone	345:369	arg1	dressings					387:395	coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings	325:395	coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings	325:395	This study deals with the designing and development of coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings for efficient simultaneous drug delivery.
27987890	5	27	theme	ferulic	803:809	arg1	acid					811:814	ferulic acid	803:814	ferulic acid	803:814	The in vitro release studies showed sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h.
27987890	5	28	theme	sustained	761:769	arg1	release					771:777	sustained release	761:777	sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h	761:855	The in vitro release studies showed sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h.
27987890	2	29	theme	electrospun	333:343	arg1	dressings					387:395	coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings	325:395	coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings	325:395	This study deals with the designing and development of coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings for efficient simultaneous drug delivery.
27987890	0	30	theme	composite	41:49	arg1	scaffold					63:70	chitosan-polycaprolactone composite nanofibrous scaffold	15:70	chitosan-polycaprolactone composite nanofibrous scaffold	15:70	Fabrication of chitosan-polycaprolactone composite nanofibrous scaffold for simultaneous delivery of ferulic acid and resveratrol.
27987890	4	31	theme	scaffold	665:672	arg1	characterization					641:656	Structural and physico-chemical characterization	609:656	Structural and physico-chemical characterization of the scaffold	609:672	Structural and physico-chemical characterization of the scaffold confirmed the encapsulation of both the molecules.
27987890	8	32	theme	wound	1191:1195	arg1	therapeutics					1197:1208	wound therapeutics	1191:1208	wound therapeutics	1191:1208	The study therefore, opens up venues for designing sequential and sustained drug delivery systems in wound therapeutics.
27987890	2	33	theme	coaxial	325:331	arg1	dressings					387:395	coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings	325:395	coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings	325:395	This study deals with the designing and development of coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings for efficient simultaneous drug delivery.
27987890	0	34	theme	chitosan-polycaprolactone	15:39	arg1	scaffold					63:70	chitosan-polycaprolactone composite nanofibrous scaffold	15:70	chitosan-polycaprolactone composite nanofibrous scaffold	15:70	Fabrication of chitosan-polycaprolactone composite nanofibrous scaffold for simultaneous delivery of ferulic acid and resveratrol.
27987890	1	35	theme	Wound	131:135	arg1	process					167:173	a complex cellular process	148:173	a complex cellular process involving various mechanisms making it intricate for designing a scaffold for therapeutic use	148:267	Wound healing is a complex cellular process involving various mechanisms making it intricate for designing a scaffold for therapeutic use.
27987890	1	35	theme	Wound	131:135	arg1	healing					137:143	Wound healing	131:143	Wound healing	131:143	Wound healing is a complex cellular process involving various mechanisms making it intricate for designing a scaffold for therapeutic use.
27987890	6	36	dep	in	935:936	arg1	vitro					938:942	vitro	938:942	vitro	938:942	Functional characterization of the nanofibrous wound dressing exhibited good in vitro and in vivo biocompatibility.
27987890	1	37	theme	cellular	158:165	arg1	process					167:173	a complex cellular process	148:173	a complex cellular process involving various mechanisms making it intricate for designing a scaffold for therapeutic use	148:267	Wound healing is a complex cellular process involving various mechanisms making it intricate for designing a scaffold for therapeutic use.
27987890	1	37	theme	cellular	158:165	arg1	healing					137:143	Wound healing	131:143	Wound healing	131:143	Wound healing is a complex cellular process involving various mechanisms making it intricate for designing a scaffold for therapeutic use.
27987890	0	38	theme	scaffold	63:70	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of chitosan-polycaprolactone composite nanofibrous scaffold for simultaneous delivery of ferulic acid and resveratrol.	0:129	Fabrication of chitosan-polycaprolactone composite nanofibrous scaffold for simultaneous delivery of ferulic acid and resveratrol.
27987890	6	39	dep	in	948:949	arg1	vivo					951:954	vivo	951:954	vivo	951:954	Functional characterization of the nanofibrous wound dressing exhibited good in vitro and in vivo biocompatibility.
27987890	0	40	theme	nanofibrous	51:61	arg1	scaffold					63:70	chitosan-polycaprolactone composite nanofibrous scaffold	15:70	chitosan-polycaprolactone composite nanofibrous scaffold	15:70	Fabrication of chitosan-polycaprolactone composite nanofibrous scaffold for simultaneous delivery of ferulic acid and resveratrol.
27987890	8	41	theme	drug	1166:1169	arg1	systems					1180:1186	sequential and sustained drug delivery systems	1141:1186	sequential and sustained drug delivery systems	1141:1186	The study therefore, opens up venues for designing sequential and sustained drug delivery systems in wound therapeutics.
27987890	0	42	theme	simultaneous	76:87	arg1	delivery					89:96	simultaneous delivery	76:96	simultaneous delivery of ferulic acid and resveratrol	76:128	Fabrication of chitosan-polycaprolactone composite nanofibrous scaffold for simultaneous delivery of ferulic acid and resveratrol.
27987890	4	43	theme	physico-chemical	624:639	arg1	characterization					641:656	Structural and physico-chemical characterization	609:656	Structural and physico-chemical characterization of the scaffold	609:672	Structural and physico-chemical characterization of the scaffold confirmed the encapsulation of both the molecules.
27987890	6	44	theme	Functional	858:867	arg1	characterization					869:884	Functional characterization	858:884	Functional characterization of the nanofibrous wound dressing	858:918	Functional characterization of the nanofibrous wound dressing exhibited good in vitro and in vivo biocompatibility.
27987890	3	45	theme	pro-angiogenic	569:582	arg1	activities					584:593	anti-inflammatory and pro-angiogenic activities	547:593	anti-inflammatory and pro-angiogenic activities respectively	547:606	Ferulic acid and resveratrol were chosen for incorporation into core-shell nanofibers for their efficacy in anti-inflammatory and pro-angiogenic activities respectively.
27987890	3	46	theme	anti-inflammatory	547:563	arg1	activities					584:593	anti-inflammatory and pro-angiogenic activities	547:593	anti-inflammatory and pro-angiogenic activities respectively	547:606	Ferulic acid and resveratrol were chosen for incorporation into core-shell nanofibers for their efficacy in anti-inflammatory and pro-angiogenic activities respectively.
27987890	3	47	from	efficacy	535:542	arg1	activities					584:593	anti-inflammatory and pro-angiogenic activities	547:593	anti-inflammatory and pro-angiogenic activities respectively	547:606	Ferulic acid and resveratrol were chosen for incorporation into core-shell nanofibers for their efficacy in anti-inflammatory and pro-angiogenic activities respectively.
27987890	5	48	dep	in	729:730	arg1	vitro					732:736	vitro	732:736	vitro	732:736	The in vitro release studies showed sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h.
27987890	5	49	theme	acid	811:814	arg1	release					771:777	sustained release	761:777	sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h	761:855	The in vitro release studies showed sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h.
27987890	6	50	theme	in	948:949	arg1	biocompatibility					956:971	good in vitro and in vivo biocompatibility	930:971	good in vitro and in vivo biocompatibility	930:971	Functional characterization of the nanofibrous wound dressing exhibited good in vitro and in vivo biocompatibility.
27987890	2	51	theme	wound	381:385	arg1	dressings					387:395	coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings	325:395	coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings	325:395	This study deals with the designing and development of coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings for efficient simultaneous drug delivery.
27987890	1	52	theme	complex	150:156	arg1	process					167:173	a complex cellular process	148:173	a complex cellular process involving various mechanisms making it intricate for designing a scaffold for therapeutic use	148:267	Wound healing is a complex cellular process involving various mechanisms making it intricate for designing a scaffold for therapeutic use.
27987890	1	52	theme	complex	150:156	arg1	healing					137:143	Wound healing	131:143	Wound healing	131:143	Wound healing is a complex cellular process involving various mechanisms making it intricate for designing a scaffold for therapeutic use.
27987890	1	53	theme	therapeutic	253:263	arg1	use					265:267	therapeutic use	253:267	therapeutic use	253:267	Wound healing is a complex cellular process involving various mechanisms making it intricate for designing a scaffold for therapeutic use.
27987890	5	54	dep	%	824:824	arg1	up					816:817	up	816:817	up	816:817	The in vitro release studies showed sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h.
27987890	5	54	dep	%	824:824	arg1	120h					852:855	till 120h	847:855	till 120h	847:855	The in vitro release studies showed sustained release of both resveratrol and ferulic acid up to 48% and 55% respectively till 120h.
27987890	8	55	theme	delivery	1171:1178	arg1	systems					1180:1186	sequential and sustained drug delivery systems	1141:1186	sequential and sustained drug delivery systems	1141:1186	The study therefore, opens up venues for designing sequential and sustained drug delivery systems in wound therapeutics.
27987890	2	56	dep	designing	296:304	arg1	the					292:294	the	292:294	the	292:294	This study deals with the designing and development of coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings for efficient simultaneous drug delivery.
27987890	3	57	theme	Ferulic	439:445	arg1	acid					447:450	Ferulic acid	439:450	Ferulic acid	439:450	Ferulic acid and resveratrol were chosen for incorporation into core-shell nanofibers for their efficacy in anti-inflammatory and pro-angiogenic activities respectively.
27987890	2	58	theme	drug	424:427	arg1	delivery					429:436	efficient simultaneous drug delivery	401:436	efficient simultaneous drug delivery	401:436	This study deals with the designing and development of coaxial electrospun Chitosan-Polycaprolactone nanofiber wound dressings for efficient simultaneous drug delivery.
25051691	0	0	theme	Tor	85:87	arg1	strains					89:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	1	1	theme	Federation	318:327	arg1	territory					297:305	the territory	293:305	the territory of Russian Federation	293:327	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	3	2	theme	temperature	673:683	arg1	C					717:717	5, 25, 37 and 42 degrees C	692:717	C	717:717	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	3	2	theme	temperature	673:683	arg1	modes					685:689	various temperature modes	665:689	various temperature modes (5, 25, 37 and 42 degrees C)	665:718	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	6	3	theme	--exopolysaccharide	1305:1323	arg1	C					1303:1303	5 degrees C	1293:1303	5 degrees C	1293:1303	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	6	3	theme	--exopolysaccharide	1305:1323	arg1	temperatures					1279:1290	lower temperatures	1273:1290	lower temperatures (5 degrees C)--exopolysaccharide	1273:1323	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	0	4	theme	El	82:83	arg1	strains					89:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	3	5	theme	autoclaved	639:648	arg1	water					656:660	autoclaved river water	639:660	autoclaved river water	639:660	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	2	6	theme	altered	556:562	arg1	variants					564:571	16--genetically altered variants	540:571	16--genetically altered variants	540:571	MATERIALS AND METHODS 24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants.
25051691	5	7	theme	degrees	998:1004	arg1	C.					1006:1007	25 degrees C.	995:1007	25 degrees C.	995:1007	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	1	8	theme	biovar	248:253	arg1	Tor					258:260	V. cholerae biovar El Tor	236:260	V. cholerae biovar El Tor	236:260	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	3	9	theme	river	650:654	arg1	water					656:660	autoclaved river water	639:660	autoclaved river water	639:660	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	5	10	theme	C.	1006:1007	arg1	temperature					980:990	the temperature	976:990	the temperature of 25 degrees C.	976:1007	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	1	11	theme	El	255:256	arg1	Tor					258:260	V. cholerae biovar El Tor	236:260	V. cholerae biovar El Tor	236:260	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	0	12	theme	strains	89:95	arg1	resistance					13:22	[Comparative resistance	0:22	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors	0:146	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	1	13	contain	Carry	154:158	arg3	absence					337:343	the absence	333:343	the absence of nutrients	333:356	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	1	13	contain	Carry	154:158	arg1	AIM					150:152	AIM	150:152	AIM	150:152	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	1	13	contain	Carry	154:158	arg2	analysis					176:183	comparative analysis	164:183	comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years	164:288	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	1	14	theme	Tor	258:260	arg1	genovariants					220:231	genovariants	220:231	genovariants	220:231	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	1	14	theme	Tor	258:260	arg1	strains					208:214	typical strains	200:214	typical strains	200:214	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	6	15	theme	high	1133:1136	arg1	temperature					1138:1148	high temperature	1133:1148	high temperature (42 degrees C)	1133:1163	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	6	15	theme	high	1133:1136	arg1	C					1162:1162	42 degrees C	1151:1162	42 degrees C	1151:1162	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	7	16	theme	higher	1450:1455	arg1	survival					1457:1464	their higher survival	1444:1464	their higher survival	1444:1464	CONCLUSION V. cholerae biovar El Tor genovariants are able to adapt to temperature change better, that may facilitate their higher survival in the environment.
25051691	5	17	theme	typical	865:871	arg1	strains					873:879	typical strains	865:879	RESULTS Genovariants as well as typical strains	833:879	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	5	18	dep	5	935:935	arg1	to					932:933	to	932:933	to	932:933	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	2	19	theme	V.	431:432	arg1	strains					457:463	24 V. cholerae biovar El Tor strains	428:463	24 V. cholerae biovar El Tor strains isolated in 1970	428:480	MATERIALS AND METHODS 24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants.
25051691	2	20	theme	Tor	453:455	arg1	strains					457:463	24 V. cholerae biovar El Tor strains	428:463	24 V. cholerae biovar El Tor strains isolated in 1970	428:480	MATERIALS AND METHODS 24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants.
25051691	2	21	dep	MATERIALS	406:414	arg1	studied					494:500	studied	494:500	were studied	489:500	MATERIALS AND METHODS 24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants.
25051691	2	21	dep	MATERIALS	406:414	arg1	strains					457:463	24 V. cholerae biovar El Tor strains	428:463	24 V. cholerae biovar El Tor strains isolated in 1970	428:480	MATERIALS AND METHODS 24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants.
25051691	6	22	theme	lower	1273:1277	arg1	C					1303:1303	5 degrees C	1293:1303	5 degrees C	1293:1303	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	6	22	theme	lower	1273:1277	arg1	temperatures					1279:1290	lower temperatures	1273:1290	lower temperatures (5 degrees C)--exopolysaccharide	1273:1323	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	1	23	theme	comparative	164:174	arg1	analysis					176:183	comparative analysis	164:183	comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years	164:288	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	3	24	theme	%	608:608	arg1	solution					626:633	0.9% sodium chloride solution	605:633	0.9% sodium chloride solution	605:633	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	1	25	theme	nutrients	348:356	arg1	absence					337:343	the absence	333:343	the absence of nutrients	333:356	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	4	26	theme	electrophoresis	793:807	arg1	method					809:814	electrophoresis method	793:814	electrophoresis method	793:814	Protein composition and exopolysaccharide production were determined by electrophoresis method by U.K. Laemmli.
25051691	3	27	theme	sodium	610:615	arg1	solution					626:633	0.9% sodium chloride solution	605:633	0.9% sodium chloride solution	605:633	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	0	28	theme	[Comparative	0:11	arg1	resistance					13:22	[Comparative resistance	0:22	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors	0:146	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	6	29	theme	membrane	1205:1212	arg1	OmpU					1214:1217	outer membrane OmpU	1199:1217	outer membrane OmpU	1199:1217	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	6	30	theme	adaptation	1119:1128	arg1	result					1109:1114	a result	1107:1114	a result of adaptation to high temperature (42 degrees C)	1107:1163	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	6	30	theme	adaptation	1119:1128	arg1	biosynthesis					1165:1176	biosynthesis	1165:1176	biosynthesis of porin proteins of outer membrane OmpU and/or OmpT	1165:1229	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	7	31	theme	biovar	1349:1354	arg1	genovariants					1363:1374	CONCLUSION V. cholerae biovar El Tor genovariants	1326:1374	CONCLUSION V. cholerae biovar El Tor genovariants	1326:1374	CONCLUSION V. cholerae biovar El Tor genovariants are able to adapt to temperature change better, that may facilitate their higher survival in the environment.
25051691	2	32	dep	V.	431:432	arg1	cholerae					434:441	cholerae	434:441	cholerae	434:441	MATERIALS AND METHODS 24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants.
25051691	0	33	theme	typical	27:33	arg1	strains					89:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	7	34	dep	V.	1337:1338	arg1	cholerae					1340:1347	cholerae	1340:1347	cholerae	1340:1347	CONCLUSION V. cholerae biovar El Tor genovariants are able to adapt to temperature change better, that may facilitate their higher survival in the environment.
25051691	0	35	theme	unfavorable	114:124	arg1	factors					140:146	unfavorable environmental factors	114:146	unfavorable environmental factors	114:146	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	6	36	theme	OmpU	1214:1217	arg1	result					1109:1114	a result	1107:1114	a result of adaptation to high temperature (42 degrees C)	1107:1163	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	6	36	theme	OmpU	1214:1217	arg1	biosynthesis					1165:1176	biosynthesis	1165:1176	biosynthesis of porin proteins of outer membrane OmpU and/or OmpT	1165:1229	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	1	37	theme	survival	188:195	arg1	analysis					176:183	comparative analysis	164:183	comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years	164:288	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	1	38	theme	different	274:282	arg1	years					284:288	different years	274:288	different years	274:288	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	6	39	theme	outer	1199:1203	arg1	OmpU					1214:1217	outer membrane OmpU	1199:1217	outer membrane OmpU	1199:1217	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	3	40	theme	0.9	605:607	arg1	%					608:608	%	608:608	%	608:608	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	5	41	dep	RESULTS	833:839	arg1	Genovariants					841:852	Genovariants	841:852	Genovariants	841:852	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	5	41	dep	RESULTS	833:839	arg1	strains					873:879	typical strains	865:879	RESULTS Genovariants as well as typical strains	833:879	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	2	42	theme	El	450:451	arg1	strains					457:463	24 V. cholerae biovar El Tor strains	428:463	24 V. cholerae biovar El Tor strains isolated in 1970	428:480	MATERIALS AND METHODS 24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants.
25051691	0	43	theme	factors	140:146	arg1	effect					104:109	the effect	100:109	the effect of unfavorable environmental factors	100:146	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	1	44	dep	V.	236:237	arg1	cholerae					239:246	cholerae	239:246	cholerae	239:246	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	1	45	theme	typical	200:206	arg1	strains					208:214	typical strains	200:214	typical strains	200:214	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	2	46	theme	biovar	443:448	arg1	strains					457:463	24 V. cholerae biovar El Tor strains	428:463	24 V. cholerae biovar El Tor strains isolated in 1970	428:480	MATERIALS AND METHODS 24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants.
25051691	3	47	theme	42	706:707	arg1	C					717:717	5, 25, 37 and 42 degrees C	692:717	C	717:717	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	3	47	theme	42	706:707	arg1	modes					685:689	various temperature modes	665:689	various temperature modes (5, 25, 37 and 42 degrees C)	665:718	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	0	48	theme	environmental	126:138	arg1	factors					140:146	unfavorable environmental factors	114:146	unfavorable environmental factors	114:146	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	5	49	theme	temperature	1084:1094	arg1	stress					1096:1101	temperature stress	1084:1101	temperature stress	1084:1101	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	7	50	theme	CONCLUSION	1326:1335	arg1	genovariants					1363:1374	CONCLUSION V. cholerae biovar El Tor genovariants	1326:1374	CONCLUSION V. cholerae biovar El Tor genovariants	1326:1374	CONCLUSION V. cholerae biovar El Tor genovariants are able to adapt to temperature change better, that may facilitate their higher survival in the environment.
25051691	1	51	theme	strains	208:214	arg1	survival					188:195	survival	188:195	survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years	188:288	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	0	52	theme	Vibrio	59:64	arg1	strains					89:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	3	53	theme	degrees	709:715	arg1	C					717:717	5, 25, 37 and 42 degrees C	692:717	C	717:717	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	3	53	theme	degrees	709:715	arg1	modes					685:689	various temperature modes	665:689	various temperature modes (5, 25, 37 and 42 degrees C)	665:718	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	6	54	theme	porin	1181:1185	arg1	proteins					1187:1194	porin proteins	1181:1194	porin proteins	1181:1194	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	6	55	theme	genovariant	1247:1257	arg1	cells					1259:1263	genovariant cells	1247:1263	genovariant cells	1247:1263	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	3	56	theme	various	665:671	arg1	C					717:717	5, 25, 37 and 42 degrees C	692:717	C	717:717	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	3	56	theme	various	665:671	arg1	modes					685:689	various temperature modes	665:689	various temperature modes (5, 25, 37 and 42 degrees C)	665:718	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	7	57	theme	temperature	1397:1407	arg1	change					1409:1414	temperature change	1397:1414	temperature change	1397:1414	CONCLUSION V. cholerae biovar El Tor genovariants are able to adapt to temperature change better, that may facilitate their higher survival in the environment.
25051691	0	58	theme	altered	51:57	arg1	strains					89:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	7	59	theme	Tor	1359:1361	arg1	genovariants					1363:1374	CONCLUSION V. cholerae biovar El Tor genovariants	1326:1374	CONCLUSION V. cholerae biovar El Tor genovariants	1326:1374	CONCLUSION V. cholerae biovar El Tor genovariants are able to adapt to temperature change better, that may facilitate their higher survival in the environment.
25051691	2	60	theme	typical	519:525	arg1	isolates					527:534	typical isolates	519:534	typical isolates	519:534	MATERIALS AND METHODS 24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants.
25051691	4	61	theme	exopolysaccharide	745:761	arg1	production					763:772	exopolysaccharide production	745:772	exopolysaccharide production	745:772	Protein composition and exopolysaccharide production were determined by electrophoresis method by U.K. Laemmli.
25051691	5	62	theme	typical	1025:1031	arg1	vibrios					1039:1045	typical eltor vibrios	1025:1045	typical eltor vibrios	1025:1045	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	7	63	theme	V.	1337:1338	arg1	CONCLUSION					1326:1335	CONCLUSION	1326:1335	CONCLUSION V. cholerae biovar El Tor genovariants	1326:1374	CONCLUSION V. cholerae biovar El Tor genovariants are able to adapt to temperature change better, that may facilitate their higher survival in the environment.
25051691	6	64	theme	degrees	1154:1160	arg1	temperature					1138:1148	high temperature	1133:1148	high temperature (42 degrees C)	1133:1163	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	6	64	theme	degrees	1154:1160	arg1	C					1162:1162	42 degrees C	1151:1162	42 degrees C	1151:1162	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	1	65	theme	temperature	386:396	arg1	stress					398:403	temperature stress	386:403	temperature stress	386:403	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	3	66	theme	5	692:692	arg1	C					717:717	5, 25, 37 and 42 degrees C	692:717	C	717:717	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	3	66	theme	5	692:692	arg1	modes					685:689	various temperature modes	665:689	various temperature modes (5, 25, 37 and 42 degrees C)	665:718	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	6	67	theme	proteins	1187:1194	arg1	result					1109:1114	a result	1107:1114	a result of adaptation to high temperature (42 degrees C)	1107:1163	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	6	67	theme	proteins	1187:1194	arg1	biosynthesis					1165:1176	biosynthesis	1165:1176	biosynthesis of porin proteins of outer membrane OmpU and/or OmpT	1165:1229	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	2	68	theme	those	508:512	arg1	8					503:503	8	503:503	8	503:503	MATERIALS AND METHODS 24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants.
25051691	2	68	theme	those	508:512	arg1	those					508:512	those	508:512	those	508:512	MATERIALS AND METHODS 24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants.
25051691	1	69	theme	stress	398:403	arg1	conditions					372:381	the conditions	368:381	the conditions of temperature stress	368:403	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	5	70	theme	long	918:921	arg1	time					923:926	a long time	916:926	a long time (up to 5 months)	916:943	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	5	70	theme	long	918:921	arg1	months					937:942	up to 5 months	929:942	up to 5 months	929:942	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	5	71	theme	nutrients	963:971	arg1	absence					952:958	the absence	948:958	the absence of nutrients	948:971	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	6	72	theme	OmpT	1226:1229	arg1	result					1109:1114	a result	1107:1114	a result of adaptation to high temperature (42 degrees C)	1107:1163	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	6	72	theme	OmpT	1226:1229	arg1	biosynthesis					1165:1176	biosynthesis	1165:1176	biosynthesis of porin proteins of outer membrane OmpU and/or OmpT	1165:1229	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	1	73	theme	genovariants	220:231	arg1	survival					188:195	survival	188:195	survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years	188:288	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
25051691	0	74	theme	biovar	75:80	arg1	strains					89:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	3	75	theme	chloride	617:624	arg1	solution					626:633	0.9% sodium chloride solution	605:633	0.9% sodium chloride solution	605:633	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	4	76	theme	Protein	721:727	arg1	composition					729:739	Protein composition	721:739	Protein composition	721:739	Protein composition and exopolysaccharide production were determined by electrophoresis method by U.K. Laemmli.
25051691	0	77	theme	cholerae	66:73	arg1	strains					89:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	typical and genetically altered Vibrio cholerae biovar El Tor strains	27:95	[Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
25051691	5	78	theme	eltor	1033:1037	arg1	vibrios					1039:1045	typical eltor vibrios	1025:1045	typical eltor vibrios	1025:1045	RESULTS Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress.
25051691	3	79	theme	Strain	574:579	arg1	survival					581:588	Strain survival	574:588	Strain survival	574:588	Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C).
25051691	6	80	theme	degrees	1295:1301	arg1	C					1303:1303	5 degrees C	1293:1303	5 degrees C	1293:1303	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	6	80	theme	degrees	1295:1301	arg1	temperatures					1279:1290	lower temperatures	1273:1290	lower temperatures (5 degrees C)--exopolysaccharide	1273:1323	As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
25051691	1	81	theme	V.	236:237	arg1	Tor					258:260	V. cholerae biovar El Tor	236:260	V. cholerae biovar El Tor	236:260	AIM Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
24731218	0	0	theme	high	90:93	arg1	modulus					103:109	high storage modulus	90:109	high storage modulus	90:109	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	6	1	theme	other	1393:1397	arg1	membranes					1407:1415	other polymer membranes	1393:1415	other polymer membranes	1393:1415	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	2	theme	proton	1508:1513	arg1	elements					1526:1533	functional proton conducting elements	1497:1533	functional proton conducting elements	1497:1533	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	2	theme	proton	1508:1513	arg1	actuators					1556:1564	actuators	1556:1564	actuators	1556:1564	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	2	theme	proton	1508:1513	arg1	sensors					1544:1550	sensors	1544:1550	sensors	1544:1550	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	1	3	theme	bacterial	262:270	arg1	cellulose					272:280	bacterial cellulose	262:280	bacterial cellulose (BC)	262:285	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	1	3	theme	bacterial	262:270	arg1	BC					283:284	BC	283:284	BC	283:284	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	6	4	theme	redox	1448:1452	arg1	batteries					1459:1467	redox flow batteries	1448:1467	redox flow batteries	1448:1467	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	4	5	theme	through-plane	961:973	arg1	conductivity					984:995	The through-plane protonic conductivity	957:995	The through-plane protonic conductivity of the best membrane	957:1016	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	6	6	theme	functional	1497:1506	arg1	elements					1526:1533	functional proton conducting elements	1497:1533	functional proton conducting elements	1497:1533	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	6	theme	functional	1497:1506	arg1	actuators					1556:1564	actuators	1556:1564	actuators	1556:1564	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	6	theme	functional	1497:1506	arg1	sensors					1544:1550	sensors	1544:1550	sensors	1544:1550	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	4	7	theme	best	1004:1007	arg1	membrane					1009:1016	the best membrane	1000:1016	the best membrane	1000:1016	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	4	8	theme	cm	1040:1041	arg1	excess					1024:1029	excess	1024:1029	excess of 0.1 S cm(-1)	1024:1045	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	2	9	theme	nanofibrilar	545:556	arg1	network					558:564	The BC nanofibrilar network	538:564	The BC nanofibrilar network	538:564	The BC nanofibrilar network endows the composite membranes with excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis.
24731218	1	10	theme	ethylene	417:424	arg1	glycol					426:431	ethylene glycol	417:431	ethylene glycol	417:431	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	1	10	theme	ethylene	417:424	arg1	poly					412:415	poly	412:415	poly(ethylene glycol) diacrylate (PEGDA)	412:451	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	6	11	theme	nanocomposites	1348:1361	arg1	potential					1329:1337	the potential	1325:1337	the potential of these nanocomposites	1325:1361	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	11	theme	nanocomposites	1348:1361	arg1	alternative					1378:1388	a bio-based alternative	1366:1388	a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators	1366:1564	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	0	12	theme	storage	95:101	arg1	modulus					103:109	high storage modulus	90:109	high storage modulus	90:109	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	4	13	theme	60	1120:1121	arg1	%					1122:1122	%	1122:1122	%	1122:1122	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	2	14	theme	BC	542:543	arg1	network					558:564	The BC nanofibrilar network	538:564	The BC nanofibrilar network	538:564	The BC nanofibrilar network endows the composite membranes with excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis.
24731218	0	15	with	membranes	75:83	arg1	modulus					103:109	high storage modulus	90:109	high storage modulus	90:109	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	0	15	with	membranes	75:83	arg1	conductivity					124:135	protonic conductivity	115:135	protonic conductivity	115:135	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	3	16	theme	sulfonic	782:789	arg1	acid					791:794	sulfonic acid	782:794	sulfonic acid groups	782:801	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	1	17	theme	ensuing	500:506	arg1	polymer					508:514	the ensuing polymer	496:514	the ensuing polymer	496:514	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	3	18	theme	large	759:763	arg1	responsible					814:824	responsible	814:824	responsible	814:824	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	3	18	theme	large	759:763	arg1	concentration					765:777	The large concentration	755:777	The large concentration of sulfonic acid groups in PSSA	755:809	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	0	19	theme	protonic	115:122	arg1	conductivity					124:135	protonic conductivity	115:135	protonic conductivity	115:135	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	3	20	theme	ionic	839:843	arg1	capacity					854:861	the high ionic exchange capacity	830:861	the high ionic exchange capacity of the composite membranes	830:888	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	2	21	theme	excellent	602:610	arg1	properties					623:632	excellent mechanical properties	602:632	excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis	602:752	The BC nanofibrilar network endows the composite membranes with excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis.
24731218	4	22	theme	%	1062:1062	arg1	humidity					1073:1080	94 °C and 98% relative humidity	1050:1080	humidity	1073:1080	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	1	23	theme	polymer	508:514	arg1	conversion					482:491	conversion	482:491	conversion of the ensuing polymer into the acidic form	482:535	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	2	24	theme	mechanical	612:621	arg1	properties					623:632	excellent mechanical properties	602:632	excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis	602:752	The BC nanofibrilar network endows the composite membranes with excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis.
24731218	4	25	theme	membrane	1009:1016	arg1	conductivity					984:995	The through-plane protonic conductivity	957:995	The through-plane protonic conductivity of the best membrane	957:1016	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	1	26	theme	in	341:342	arg1	polymerization					362:375	in situ free radical polymerization	341:375	in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form	341:535	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	3	27	theme	wt	940:941	arg1	%					943:943	83 wt % PSSA/PEGDA	937:954	83 wt % PSSA/PEGDA	937:954	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	4	28	theme	S	1038:1038	arg1	-1					1043:1044	-1	1043:1044	-1	1043:1044	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	4	28	theme	S	1038:1038	arg1	cm					1040:1041	0.1 S cm	1034:1041	0.1 S cm(-1)	1034:1045	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	2	29	from	°C	653:654	arg1	properties					623:632	excellent mechanical properties	602:632	excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis	602:752	The BC nanofibrilar network endows the composite membranes with excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis.
24731218	0	30	theme	bacterial	15:23	arg1	cellulose-poly					25:38	Nanostructured bacterial cellulose-poly	0:38	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.	0:136	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	0	30	theme	bacterial	15:23	arg1	acid					59:62	4-styrene sulfonic acid	40:62	4-styrene sulfonic acid	40:62	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	4	31	theme	%	1122:1122	arg1	RH					1124:1125	60% RH	1120:1125	60% RH	1120:1125	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	2	32	theme	composite	577:585	arg1	membranes					587:595	the composite membranes	573:595	the composite membranes	573:595	The BC nanofibrilar network endows the composite membranes with excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis.
24731218	4	33	from	humidity	1073:1080	arg1	excess					1024:1029	excess	1024:1029	excess of 0.1 S cm(-1)	1024:1045	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	3	34	theme	high	834:837	arg1	capacity					854:861	the high ionic exchange capacity	830:861	the high ionic exchange capacity of the composite membranes	830:888	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	1	35	theme	new	185:187	arg1	generation					189:198	a new generation	183:198	a new generation	183:198	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	0	36	theme	Nanostructured	0:13	arg1	cellulose-poly					25:38	Nanostructured bacterial cellulose-poly	0:38	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.	0:136	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	0	36	theme	Nanostructured	0:13	arg1	acid					59:62	4-styrene sulfonic acid	40:62	4-styrene sulfonic acid	40:62	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	6	37	from	application	1421:1431	arg1	devices					1479:1485	other devices	1473:1485	other devices	1473:1485	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	37	from	application	1421:1431	arg1	batteries					1459:1467	redox flow batteries	1448:1467	redox flow batteries	1448:1467	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	37	from	application	1421:1431	arg1	cells					1441:1445	fuel cells	1436:1445	fuel cells	1436:1445	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	38	theme	flow	1454:1457	arg1	batteries					1459:1467	redox flow batteries	1448:1467	redox flow batteries	1448:1467	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	1	39	theme	acidic	525:530	arg1	form					532:535	the acidic form	521:535	the acidic form	521:535	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	1	40	theme	generation	189:198	arg1	development					168:178	the development	164:178	the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form	164:535	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	4	41	theme	relative	1064:1071	arg1	humidity					1073:1080	94 °C and 98% relative humidity	1050:1080	humidity	1073:1080	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	3	42	theme	composite	870:878	arg1	membranes					880:888	the composite membranes	866:888	the composite membranes	866:888	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	0	43	theme	cellulose-poly	25:38	arg1	membranes					75:83	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes	0:83	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.	0:136	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	1	44	theme	free	349:352	arg1	polymerization					362:375	in situ free radical polymerization	341:375	in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form	341:535	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	6	45	theme	low	1305:1307	arg1	cost					1309:1312	low cost	1305:1312	low cost	1305:1312	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	1	46	theme	bio-based	203:211	arg1	membranes					243:251	bio-based nanocomposite proton exchange membranes	203:251	bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA)	203:326	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	0	47	theme	sulfonic	50:57	arg1	cellulose-poly					25:38	Nanostructured bacterial cellulose-poly	0:38	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.	0:136	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	0	47	theme	sulfonic	50:57	arg1	acid					59:62	4-styrene sulfonic acid	40:62	4-styrene sulfonic acid	40:62	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	3	48	theme	exchange	845:852	arg1	capacity					854:861	the high ionic exchange capacity	830:861	the high ionic exchange capacity of the composite membranes	830:888	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	1	49	dep	in	341:342	arg1	situ					344:347	situ	344:347	situ	344:347	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	1	50	theme	radical	354:360	arg1	polymerization					362:375	in situ free radical polymerization	341:375	in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form	341:535	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	3	51	theme	acid	791:794	arg1	groups					796:801	sulfonic acid groups	782:801	sulfonic acid groups	782:801	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	1	52	theme	nanocomposite	213:225	arg1	membranes					243:251	bio-based nanocomposite proton exchange membranes	203:251	bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA)	203:326	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	0	53	theme	4-styrene	40:48	arg1	cellulose-poly					25:38	Nanostructured bacterial cellulose-poly	0:38	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.	0:136	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	0	53	theme	4-styrene	40:48	arg1	acid					59:62	4-styrene sulfonic acid	40:62	4-styrene sulfonic acid	40:62	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	1	54	theme	4-styrene	296:304	arg1	poly					291:294	poly	291:294	poly(4-styrene sulfonic acid) (PSSA)	291:326	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	1	54	theme	4-styrene	296:304	arg1	acid					315:318	4-styrene sulfonic acid	296:318	4-styrene sulfonic acid	296:318	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	4	55	from	°C	1053:1054	arg1	excess					1024:1029	excess	1024:1029	excess of 0.1 S cm(-1)	1024:1045	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	6	56	theme	polymer	1399:1405	arg1	membranes					1407:1415	other polymer membranes	1393:1415	other polymer membranes	1393:1415	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	1	57	theme	proton	227:232	arg1	membranes					243:251	bio-based nanocomposite proton exchange membranes	203:251	bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA)	203:326	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	6	58	theme	fuel	1436:1439	arg1	cells					1441:1445	fuel cells	1436:1445	fuel cells	1436:1445	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	1	59	theme	sulfonic	306:313	arg1	poly					291:294	poly	291:294	poly(4-styrene sulfonic acid) (PSSA)	291:326	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	1	59	theme	sulfonic	306:313	arg1	acid					315:318	4-styrene sulfonic acid	296:318	4-styrene sulfonic acid	296:318	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	6	60	theme	properties	1289:1298	arg1	combination					1248:1258	This combination	1243:1258	This combination of electric and viscoelastic properties with low cost	1243:1312	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	61	theme	other	1473:1477	arg1	devices					1479:1485	other devices	1473:1485	other devices	1473:1485	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	62	theme	conducting	1515:1524	arg1	elements					1526:1533	functional proton conducting elements	1497:1533	functional proton conducting elements	1497:1533	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	62	theme	conducting	1515:1524	arg1	actuators					1556:1564	actuators	1556:1564	actuators	1556:1564	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	62	theme	conducting	1515:1524	arg1	sensors					1544:1550	sensors	1544:1550	sensors	1544:1550	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	2	63	dep	140	649:651	arg1	to					646:647	to	646:647	to	646:647	The BC nanofibrilar network endows the composite membranes with excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis.
24731218	3	64	from	concentration	765:777	arg1	PSSA					806:809	PSSA	806:809	PSSA	806:809	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	1	65	theme	sodium	380:385	arg1	4-styrenesulfonate					387:404	sodium 4-styrenesulfonate	380:404	sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form	380:535	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	6	66	theme	electric	1263:1270	arg1	properties					1289:1298	electric and viscoelastic properties	1263:1298	properties	1289:1298	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	3	67	theme	membranes	880:888	arg1	capacity					854:861	the high ionic exchange capacity	830:861	the high ionic exchange capacity of the composite membranes	830:888	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	6	68	with	combination	1248:1258	arg1	cost					1309:1312	low cost	1305:1312	low cost	1305:1312	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	3	69	theme	groups	796:801	arg1	responsible					814:824	responsible	814:824	responsible	814:824	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	3	69	theme	groups	796:801	arg1	concentration					765:777	The large concentration	755:777	The large concentration of sulfonic acid groups in PSSA	755:809	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	3	70	with	composite	922:930	arg1	%					943:943	83 wt % PSSA/PEGDA	937:954	83 wt % PSSA/PEGDA	937:954	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	1	71	theme	4-styrenesulfonate	387:404	arg1	polymerization					362:375	in situ free radical polymerization	341:375	in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form	341:535	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	6	72	theme	viscoelastic	1276:1287	arg1	properties					1289:1298	electric and viscoelastic properties	1263:1298	properties	1289:1298	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	4	73	theme	protonic	975:982	arg1	conductivity					984:995	The through-plane protonic conductivity	957:995	The through-plane protonic conductivity of the best membrane	957:1016	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	1	74	theme	exchange	234:241	arg1	membranes					243:251	bio-based nanocomposite proton exchange membranes	203:251	bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA)	203:326	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	4	75	theme	S	1108:1108	arg1	-1					1113:1114	-1	1113:1114	-1	1113:1114	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	4	75	theme	S	1108:1108	arg1	cm					1110:1111	0.042 S cm	1102:1111	0.042 S cm(-1)	1102:1115	The through-plane protonic conductivity of the best membrane is in excess of 0.1 S cm(-1) at 94 °C and 98% relative humidity (RH), decreasing to 0.042 S cm(-1) at 60% RH.
24731218	6	76	theme	bio-based	1368:1376	arg1	potential					1329:1337	the potential	1325:1337	the potential of these nanocomposites	1325:1361	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	6	76	theme	bio-based	1368:1376	arg1	alternative					1378:1388	a bio-based alternative	1366:1388	a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators	1366:1564	This combination of electric and viscoelastic properties with low cost underlines the potential of these nanocomposites as a bio-based alternative to other polymer membranes for application in fuel cells, redox flow batteries, or other devices requiring functional proton conducting elements, such as sensors and actuators.
24731218	1	77	theme	present	142:148	arg1	study					150:154	The present study	138:154	The present study	138:154	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	2	78	theme	mechanical	734:743	arg1	analysis					745:752	dynamic mechanical analysis	726:752	dynamic mechanical analysis	726:752	The BC nanofibrilar network endows the composite membranes with excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis.
24731218	1	79	theme	membranes	243:251	arg1	generation					189:198	a new generation	183:198	a new generation	183:198	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	1	80	theme	poly	412:415	arg1	PEGDA					446:450	PEGDA	446:450	PEGDA	446:450	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	1	80	theme	poly	412:415	arg1	diacrylate					434:443	poly(ethylene glycol) diacrylate	412:443	poly(ethylene glycol) diacrylate (PEGDA)	412:451	The present study reports the development of a new generation of bio-based nanocomposite proton exchange membranes based on bacterial cellulose (BC) and poly(4-styrene sulfonic acid) (PSSA), produced by in situ free radical polymerization of sodium 4-styrenesulfonate using poly(ethylene glycol) diacrylate (PEGDA) as cross-linker, followed by conversion of the ensuing polymer into the acidic form.
24731218	2	81	theme	pure	684:687	arg1	PSSA					689:692	pure PSSA	684:692	pure PSSA	684:692	The BC nanofibrilar network endows the composite membranes with excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis.
24731218	2	82	theme	dynamic	726:732	arg1	analysis					745:752	dynamic mechanical analysis	726:752	dynamic mechanical analysis	726:752	The BC nanofibrilar network endows the composite membranes with excellent mechanical properties at least up to 140 °C, a temperature where either pure PSSA or Nafion are soft, as shown by dynamic mechanical analysis.
24731218	0	83	theme	composite	65:73	arg1	membranes					75:83	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes	0:83	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.	0:136	Nanostructured bacterial cellulose-poly(4-styrene sulfonic acid) composite membranes with high storage modulus and protonic conductivity.
24731218	3	84	theme	mmol	905:908	arg1	g					910:910	2.25 mmol g	900:910	2.25 mmol g(-1)	900:914	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
24731218	3	84	theme	mmol	905:908	arg1	-1					912:913	-1	912:913	-1	912:913	The large concentration of sulfonic acid groups in PSSA is responsible for the high ionic exchange capacity of the composite membranes, reaching 2.25 mmol g(-1) for a composite with 83 wt % PSSA/PEGDA.
28440301	0	0	theme	similar	102:108	arg1	properties					121:130	similar pathogenic properties	102:130	similar pathogenic properties	102:130	The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans.
28440301	10	1	theme	fungal	1520:1525	arg1	pathogens					1527:1535	new fungal pathogens	1516:1535	new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans	1516:1630	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	7	2	theme	immune	1076:1081	arg1	mediators					1083:1091	immune mediators	1076:1091	immune mediators	1076:1091	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	3	3	theme	polysaccharides	546:560	arg1	secretion					533:541	secretion	533:541	secretion	533:541	The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS).
28440301	3	3	theme	polysaccharides	546:560	arg1	factor					504:509	The main Cryptococcus virulence factor	472:509	The main Cryptococcus virulence factor	472:509	The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS).
28440301	3	3	theme	polysaccharides	546:560	arg1	production					518:527	production	518:527	production	518:527	The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS).
28440301	7	4	theme	mediators	1083:1091	arg1	mediators					1083:1091	immune mediators	1076:1091	immune mediators	1076:1091	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	7	4	theme	mediators	1083:1091	arg1	variety					1065:1071	a variety	1063:1071	a variety of immune mediators	1063:1091	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	5	5	from	liquefaciens	740:751	arg1	activity					695:702	biological activity	684:702	biological activity	684:702	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	5	5	from	liquefaciens	740:751	arg1	ultrastructure					665:678	ultrastructure	665:678	ultrastructure	665:678	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	5	5	from	liquefaciens	740:751	arg1	PS					729:730	capsular and secreted PS	707:730	capsular and secreted PS from C. liquefaciens, and yeast pathogenicity	707:776	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	5	6	theme	biological	684:693	arg1	activity					695:702	biological activity	684:702	biological activity	684:702	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	2	7	theme	environmental	395:407	arg1	species					409:415	environmental species	395:415	environmental species	395:415	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	10	8	theme	transitional	1583:1594	arg1	step					1596:1599	an important transitional step	1570:1599	an important transitional step towards pathogenesis in humans	1570:1630	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	10	8	theme	transitional	1583:1594	arg1	thermotolerance					1547:1561	thermotolerance	1547:1561	thermotolerance	1547:1561	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	6	9	theme	Ultrastructural	837:851	arg1	analysis					853:860	Ultrastructural analysis	837:860	Ultrastructural analysis by high-resolution microscopy	837:890	Ultrastructural analysis by high-resolution microscopy showed that both species produce large and complex capsules.
28440301	2	10	theme	main	330:333	arg1	neoformans					287:296	Cryptococcus neoformans	274:296	Cryptococcus neoformans	274:296	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	2	10	theme	main	330:333	arg1	gattii					315:320	Cryptococcus gattii	302:320	Cryptococcus gattii	302:320	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	2	10	theme	main	330:333	arg1	agents					335:340	the main agents	326:340	the main agents of human cryptococcosis	326:364	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	9	11	theme	liquefaciens	1211:1222	arg1	PS					1224:1225	C. liquefaciens PS	1208:1225	C. liquefaciens PS	1208:1225	Treatment with C. liquefaciens PS could not protect mice against infection with C. neoformans.
28440301	4	12	theme	similar	622:628	arg1	composition					630:640	similar composition	622:640	similar composition	622:640	Previously, we showed that both species produce PS of similar composition.
28440301	0	13	theme	pathogenic	110:119	arg1	properties					121:130	similar pathogenic properties	102:130	similar pathogenic properties	102:130	The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans.
28440301	10	14	theme	biological	1408:1417	arg1	properties					1419:1428	strikingly similar ultrastructural and biological properties	1369:1428	strikingly similar ultrastructural and biological properties	1369:1428	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	0	15	with	polysaccharides	81:95	arg1	properties					121:130	similar pathogenic properties	102:130	similar pathogenic properties	102:130	The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans.
28440301	10	16	theme	C.	1348:1349	arg1	liquefaciens					1351:1362	the environmental yeast C. liquefaciens	1324:1362	the environmental yeast C. liquefaciens	1324:1362	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	8	17	theme	G.	1171:1172	arg1	lethality					1158:1166	complete lethality	1149:1166	complete lethality of G. mellonella larvae	1149:1190	Challenge with C. liquefaciens or C. neoformans led to complete lethality of G. mellonella larvae.
28440301	6	18	theme	complex	935:941	arg1	capsules					943:950	large and complex capsules	925:950	large and complex capsules	925:950	Ultrastructural analysis by high-resolution microscopy showed that both species produce large and complex capsules.
28440301	7	19	theme	phagocytosis	1007:1018	arg1	levels					1020:1025	phagocytosis levels	1007:1025	phagocytosis levels	1007:1025	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	10	20	theme	yeast	1342:1346	arg1	liquefaciens					1351:1362	the environmental yeast C. liquefaciens	1324:1362	the environmental yeast C. liquefaciens	1324:1362	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	3	21	theme	main	476:479	arg1	factor					504:509	The main Cryptococcus virulence factor	472:509	The main Cryptococcus virulence factor	472:509	The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS).
28440301	3	21	theme	main	476:479	arg1	production					518:527	production	518:527	production	518:527	The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS).
28440301	10	22	from	step	1596:1599	arg1	humans					1625:1630	humans	1625:1630	humans	1625:1630	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	7	23	theme	indistinguishable	978:994	arg1	effects					996:1002	indistinguishable effects	978:1002	indistinguishable effects	978:1002	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	5	24	with	comparison	806:815	arg1	neoformans					825:834	C. neoformans	822:834	C. neoformans	822:834	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	10	25	theme	environmental	1328:1340	arg1	liquefaciens					1351:1362	the environmental yeast C. liquefaciens	1324:1362	the environmental yeast C. liquefaciens	1324:1362	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	3	26	theme	Cryptococcus	481:492	arg1	factor					504:509	The main Cryptococcus virulence factor	472:509	The main Cryptococcus virulence factor	472:509	The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS).
28440301	3	26	theme	Cryptococcus	481:492	arg1	production					518:527	production	518:527	production	518:527	The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS).
28440301	0	27	theme	environmental	4:16	arg1	liquefaciens					37:48	The environmental yeast Cryptococcus liquefaciens	0:48	The environmental yeast Cryptococcus liquefaciens	0:48	The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans.
28440301	6	28	theme	large	925:929	arg1	capsules					943:950	large and complex capsules	925:950	large and complex capsules	925:950	Ultrastructural analysis by high-resolution microscopy showed that both species produce large and complex capsules.
28440301	8	29	dep	G.	1171:1172	arg1	larvae					1185:1190	G. mellonella larvae	1171:1190	G. mellonella larvae	1171:1190	Challenge with C. liquefaciens or C. neoformans led to complete lethality of G. mellonella larvae.
28440301	2	30	theme	cryptococcosis	351:364	arg1	neoformans					287:296	Cryptococcus neoformans	274:296	Cryptococcus neoformans	274:296	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	2	30	theme	cryptococcosis	351:364	arg1	gattii					315:320	Cryptococcus gattii	302:320	Cryptococcus gattii	302:320	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	2	30	theme	cryptococcosis	351:364	arg1	agents					335:340	the main agents	326:340	the main agents of human cryptococcosis	326:364	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	10	31	contain	have	1364:1367	arg2	properties					1419:1428	strikingly similar ultrastructural and biological properties	1369:1428	strikingly similar ultrastructural and biological properties	1369:1428	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	10	31	contain	have	1364:1367	arg1	polysaccharides					1305:1319	polysaccharides	1305:1319	polysaccharides of the environmental yeast C. liquefaciens	1305:1362	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	2	32	theme	C.	426:427	arg1	liquefaciens					429:440	C. liquefaciens	426:440	C. liquefaciens	426:440	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	9	33	with	infection	1258:1266	arg1	neoformans					1276:1285	C. neoformans	1273:1285	C. neoformans	1273:1285	Treatment with C. liquefaciens PS could not protect mice against infection with C. neoformans.
28440301	8	34	theme	C.	1109:1110	arg1	liquefaciens					1112:1123	C. liquefaciens	1109:1123	C. liquefaciens	1109:1123	Challenge with C. liquefaciens or C. neoformans led to complete lethality of G. mellonella larvae.
28440301	2	35	theme	human	345:349	arg1	cryptococcosis					351:364	human cryptococcosis	345:364	human cryptococcosis	345:364	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	10	36	theme	similar	1380:1386	arg1	properties					1419:1428	strikingly similar ultrastructural and biological properties	1369:1428	strikingly similar ultrastructural and biological properties	1369:1428	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	10	37	theme	new	1516:1518	arg1	pathogens					1527:1535	new fungal pathogens	1516:1535	new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans	1516:1630	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	0	38	theme	Cryptococcus	24:35	arg1	liquefaciens					37:48	The environmental yeast Cryptococcus liquefaciens	0:48	The environmental yeast Cryptococcus liquefaciens	0:48	The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans.
28440301	5	39	from	ultrastructure	665:678	arg1	pathogenicity					764:776	yeast pathogenicity	758:776	yeast pathogenicity	758:776	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	5	39	from	ultrastructure	665:678	arg1	liquefaciens					740:751	C. liquefaciens	737:751	C. liquefaciens	737:751	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	10	40	theme	important	1573:1581	arg1	step					1596:1599	an important transitional step	1570:1599	an important transitional step towards pathogenesis in humans	1570:1630	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	10	40	theme	important	1573:1581	arg1	thermotolerance					1547:1561	thermotolerance	1547:1561	thermotolerance	1547:1561	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	10	41	theme	pathogens	1527:1535	arg1	emergence					1503:1511	the emergence	1499:1511	the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans	1499:1630	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	0	42	theme	yeast	18:22	arg1	liquefaciens					37:48	The environmental yeast Cryptococcus liquefaciens	0:48	The environmental yeast Cryptococcus liquefaciens	0:48	The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans.
28440301	5	43	theme	C.	737:738	arg1	liquefaciens					740:751	C. liquefaciens	737:751	C. liquefaciens	737:751	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	5	44	from	pathogenicity	764:776	arg1	activity					695:702	biological activity	684:702	biological activity	684:702	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	5	44	from	pathogenicity	764:776	arg1	ultrastructure					665:678	ultrastructure	665:678	ultrastructure	665:678	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	5	44	from	pathogenicity	764:776	arg1	PS					729:730	capsular and secreted PS	707:730	capsular and secreted PS from C. liquefaciens, and yeast pathogenicity	707:776	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	10	45	theme	liquefaciens	1351:1362	arg1	polysaccharides					1305:1319	polysaccharides	1305:1319	polysaccharides of the environmental yeast C. liquefaciens	1305:1362	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	1	46	theme	growing	219:225	arg1	infections					175:184	Invasive fungal infections	159:184	Invasive fungal infections	159:184	Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients.
28440301	1	46	theme	growing	219:225	arg1	threat					227:232	a growing threat	217:232	a growing threat to immunocompromised patients	217:262	Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients.
28440301	1	46	theme	growing	219:225	arg1	cryptococcosis					197:210	cryptococcosis	197:210	cryptococcosis	197:210	Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients.
28440301	8	47	theme	complete	1149:1156	arg1	lethality					1158:1166	complete lethality	1149:1166	complete lethality of G. mellonella larvae	1149:1190	Challenge with C. liquefaciens or C. neoformans led to complete lethality of G. mellonella larvae.
28440301	8	48	with	Challenge	1094:1102	arg1	neoformans					1131:1140	C. neoformans	1128:1140	C. neoformans	1128:1140	Challenge with C. liquefaciens or C. neoformans led to complete lethality of G. mellonella larvae.
28440301	8	48	with	Challenge	1094:1102	arg1	liquefaciens					1112:1123	C. liquefaciens	1109:1123	C. liquefaciens	1109:1123	Challenge with C. liquefaciens or C. neoformans led to complete lethality of G. mellonella larvae.
28440301	3	49	theme	virulence	494:502	arg1	factor					504:509	The main Cryptococcus virulence factor	472:509	The main Cryptococcus virulence factor	472:509	The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS).
28440301	3	49	theme	virulence	494:502	arg1	production					518:527	production	518:527	production	518:527	The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS).
28440301	5	50	dep	ultrastructure	665:678	arg1	the					661:663	the	661:663	the	661:663	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	5	51	from	activity	695:702	arg1	pathogenicity					764:776	yeast pathogenicity	758:776	yeast pathogenicity	758:776	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	5	51	from	activity	695:702	arg1	liquefaciens					740:751	C. liquefaciens	737:751	C. liquefaciens	737:751	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	7	52	contain	had	974:976	arg1	PS					953:954	PS	953:954	PS from both species	953:972	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	7	52	contain	had	974:976	arg2	effects					996:1002	indistinguishable effects	978:1002	indistinguishable effects	978:1002	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	5	53	theme	capsular	707:714	arg1	PS					729:730	capsular and secreted PS	707:730	capsular and secreted PS from C. liquefaciens, and yeast pathogenicity	707:776	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	5	54	theme	invertebrate	784:795	arg1	host					797:800	an invertebrate host	781:800	an invertebrate host	781:800	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	7	55	theme	variety	1065:1071	arg1	levels					1020:1025	phagocytosis levels	1007:1025	phagocytosis levels	1007:1025	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	7	55	theme	variety	1065:1071	arg1	production					1031:1040	NO production	1028:1040	NO production	1028:1040	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	7	55	theme	variety	1065:1071	arg1	secretion					1050:1058	the secretion	1046:1058	the secretion of a variety of immune mediators	1046:1091	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	7	56	from	species	966:972	arg1	PS					953:954	PS	953:954	PS from both species	953:972	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	0	57	theme	capsular	59:66	arg1	polysaccharides					81:95	capsular and secreted polysaccharides	59:95	capsular and secreted polysaccharides with similar pathogenic properties	59:130	The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans.
28440301	5	58	theme	secreted	720:727	arg1	PS					729:730	capsular and secreted PS	707:730	capsular and secreted PS from C. liquefaciens, and yeast pathogenicity	707:776	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	2	59	theme	Cryptococcus	302:313	arg1	neoformans					287:296	Cryptococcus neoformans	274:296	Cryptococcus neoformans	274:296	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	2	59	theme	Cryptococcus	302:313	arg1	gattii					315:320	Cryptococcus gattii	302:320	Cryptococcus gattii	302:320	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	2	59	theme	Cryptococcus	302:313	arg1	agents					335:340	the main agents	326:340	the main agents of human cryptococcosis	326:364	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	10	60	theme	ultrastructural	1388:1402	arg1	properties					1419:1428	strikingly similar ultrastructural and biological properties	1369:1428	strikingly similar ultrastructural and biological properties	1369:1428	We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.
28440301	2	61	theme	opportunistic	367:379	arg1	infections					381:390	opportunistic infections	367:390	opportunistic infections	367:390	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	2	61	theme	opportunistic	367:379	arg1	liquefaciens					429:440	C. liquefaciens	426:440	C. liquefaciens	426:440	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	9	62	theme	C.	1208:1209	arg1	PS					1224:1225	C. liquefaciens PS	1208:1225	C. liquefaciens PS	1208:1225	Treatment with C. liquefaciens PS could not protect mice against infection with C. neoformans.
28440301	1	63	theme	immunocompromised	237:253	arg1	patients					255:262	immunocompromised patients	237:262	immunocompromised patients	237:262	Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients.
28440301	5	64	theme	PS	729:730	arg1	ultrastructure					665:678	ultrastructure	665:678	ultrastructure	665:678	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	5	64	theme	PS	729:730	arg1	activity					695:702	biological activity	684:702	biological activity	684:702	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28440301	6	65	theme	high-resolution	865:879	arg1	microscopy					881:890	high-resolution microscopy	865:890	high-resolution microscopy	865:890	Ultrastructural analysis by high-resolution microscopy showed that both species produce large and complex capsules.
28440301	3	66	dep	production	518:527	arg1	the					514:516	the	514:516	the	514:516	The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS).
28440301	4	67	theme	composition	630:640	arg1	PS					616:617	PS	616:617	PS of similar composition	616:640	Previously, we showed that both species produce PS of similar composition.
28440301	1	68	theme	Invasive	159:166	arg1	threat					227:232	a growing threat	217:232	a growing threat to immunocompromised patients	217:262	Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients.
28440301	1	68	theme	Invasive	159:166	arg1	infections					175:184	Invasive fungal infections	159:184	Invasive fungal infections	159:184	Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients.
28440301	1	68	theme	Invasive	159:166	arg1	cryptococcosis					197:210	cryptococcosis	197:210	cryptococcosis	197:210	Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients.
28440301	9	69	with	Treatment	1193:1201	arg1	PS					1224:1225	C. liquefaciens PS	1208:1225	C. liquefaciens PS	1208:1225	Treatment with C. liquefaciens PS could not protect mice against infection with C. neoformans.
28440301	0	70	theme	secreted	72:79	arg1	polysaccharides					81:95	capsular and secreted polysaccharides	59:95	capsular and secreted polysaccharides with similar pathogenic properties	59:130	The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans.
28440301	2	71	theme	Cryptococcus	274:285	arg1	neoformans					287:296	Cryptococcus neoformans	274:296	Cryptococcus neoformans	274:296	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	2	71	theme	Cryptococcus	274:285	arg1	gattii					315:320	Cryptococcus gattii	302:320	Cryptococcus gattii	302:320	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	2	71	theme	Cryptococcus	274:285	arg1	agents					335:340	the main agents	326:340	the main agents of human cryptococcosis	326:364	Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently.
28440301	7	72	theme	NO	1028:1029	arg1	production					1031:1040	NO production	1028:1040	NO production	1028:1040	PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators.
28440301	1	73	theme	fungal	168:173	arg1	threat					227:232	a growing threat	217:232	a growing threat to immunocompromised patients	217:262	Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients.
28440301	1	73	theme	fungal	168:173	arg1	infections					175:184	Invasive fungal infections	159:184	Invasive fungal infections	159:184	Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients.
28440301	1	73	theme	fungal	168:173	arg1	cryptococcosis					197:210	cryptococcosis	197:210	cryptococcosis	197:210	Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients.
28440301	5	74	theme	yeast	758:762	arg1	pathogenicity					764:776	yeast pathogenicity	758:776	yeast pathogenicity	758:776	Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans.
28821138	3	0	theme	chitin	521:526	arg1	solution					528:535	chitin solution	521:535	chitin solution in 11wt% NaOH/4wt% urea aqueous system	521:574	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	7	1	theme	neurites	1474:1481	arg1	percentage					1445:1454	the percentage	1441:1454	the percentage	1441:1454	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	7	1	theme	neurites	1474:1481	arg1	length					1464:1469	the length	1460:1469	the length of neurites	1460:1481	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	5	2	theme	hydrogels	960:968	arg1	elongation					916:925	elongation	916:925	elongation	916:925	The tensile strength and elongation at break of the Ch/CNT composite hydrogels were obviously enhanced, and the swelling ratio decreased.
28821138	5	2	theme	hydrogels	960:968	arg1	strength					903:910	tensile strength	895:910	tensile strength	895:910	The tensile strength and elongation at break of the Ch/CNT composite hydrogels were obviously enhanced, and the swelling ratio decreased.
28821138	8	3	theme	growth	1590:1595	arg1	substrates					1597:1606	neuronal growth substrates	1581:1606	neuronal growth substrates for the potential application in nerve regeneration	1581:1658	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	8	3	theme	growth	1590:1595	arg1	hydrogels					1568:1576	the novel Ch/CNT hydrogels	1551:1576	the novel Ch/CNT hydrogels	1551:1576	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	7	4	theme	great	1418:1422	arg1	increase					1424:1431	a great increase	1416:1431	a great increase in both the percentage and the length of neurites	1416:1481	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	3	5	from	solution	528:535	arg1	system					569:574	11wt% NaOH/4wt% urea aqueous system	540:574	11wt% NaOH/4wt% urea aqueous system	540:574	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	2	6	theme	biological	296:305	arg1	systems					307:313	biological systems	296:313	biological systems	296:313	However, the potential cytotoxicity in biological systems severely restricted the utilization of CNTs as substrates for neural growth.
28821138	4	7	theme	nanofibrous	747:757	arg1	network					759:765	a compact and neat Ch/CNT nanofibrous network	721:765	a compact and neat Ch/CNT nanofibrous network	721:765	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	4	8	theme	Ch/CNT	740:745	arg1	network					759:765	a compact and neat Ch/CNT nanofibrous network	721:765	a compact and neat Ch/CNT nanofibrous network	721:765	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	8	9	theme	Ch/CNT	1561:1566	arg1	substrates					1597:1606	neuronal growth substrates	1581:1606	neuronal growth substrates for the potential application in nerve regeneration	1581:1658	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	8	9	theme	Ch/CNT	1561:1566	arg1	hydrogels					1568:1576	the novel Ch/CNT hydrogels	1551:1576	the novel Ch/CNT hydrogels	1551:1576	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	3	10	theme	urea	556:559	arg1	system					569:574	11wt% NaOH/4wt% urea aqueous system	540:574	11wt% NaOH/4wt% urea aqueous system	540:574	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	3	11	theme	modified	502:509	arg1	CNTs					511:514	modified CNTs	502:514	modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system	502:574	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	8	12	theme	novel	1555:1559	arg1	substrates					1597:1606	neuronal growth substrates	1581:1606	neuronal growth substrates for the potential application in nerve regeneration	1581:1658	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	8	12	theme	novel	1555:1559	arg1	hydrogels					1568:1576	the novel Ch/CNT hydrogels	1551:1576	the novel Ch/CNT hydrogels	1551:1576	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	6	13	theme	good	1073:1076	arg1	hemocompatibility					1078:1094	good hemocompatibility	1073:1094	good hemocompatibility	1073:1094	In addition, the Ch/CNT hydrogels exhibited good hemocompatibility, biodegradation in vitro and biocompatibility without cytotoxicity and neurotoxicity nature to neuronal and Schwann cells (PC12 cells and RSC96 cells).
28821138	3	14	theme	%	544:544	arg1	%					554:554	11wt% NaOH/4wt%	540:554	11wt% NaOH/4wt% urea aqueous system	540:574	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	6	15	dep	cells	1212:1216	arg1	cells					1224:1228	PC12 cells	1219:1228	PC12 cells	1219:1228	In addition, the Ch/CNT hydrogels exhibited good hemocompatibility, biodegradation in vitro and biocompatibility without cytotoxicity and neurotoxicity nature to neuronal and Schwann cells (PC12 cells and RSC96 cells).
28821138	6	15	dep	cells	1212:1216	arg1	cells					1240:1244	RSC96 cells	1234:1244	RSC96 cells	1234:1244	In addition, the Ch/CNT hydrogels exhibited good hemocompatibility, biodegradation in vitro and biocompatibility without cytotoxicity and neurotoxicity nature to neuronal and Schwann cells (PC12 cells and RSC96 cells).
28821138	7	16	theme	outgrowth	1383:1391	arg1	enhancement					1314:1324	significant enhancement	1302:1324	significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells	1302:1409	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	3	17	theme	biocompatible	407:419	arg1	hydrogels					464:472	biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels	407:472	biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels	407:472	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	4	18	theme	amphiphilic	861:871	arg1	etc					886:888	etc	886:888	etc	886:888	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	4	18	theme	amphiphilic	861:871	arg1	interaction					873:883	amphiphilic interaction	861:883	amphiphilic interaction	861:883	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	7	19	theme	proliferation	1357:1369	arg1	enhancement					1314:1324	significant enhancement	1302:1324	significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells	1302:1409	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	2	20	theme	potential	270:278	arg1	cytotoxicity					280:291	the potential cytotoxicity	266:291	the potential cytotoxicity in biological systems	266:313	However, the potential cytotoxicity in biological systems severely restricted the utilization of CNTs as substrates for neural growth.
28821138	3	21	theme	composite	454:462	arg1	hydrogels					464:472	biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels	407:472	biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels	407:472	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	3	22	with	CNTs	511:514	arg1	solution					528:535	chitin solution	521:535	chitin solution in 11wt% NaOH/4wt% urea aqueous system	521:574	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	7	23	theme	significant	1302:1312	arg1	enhancement					1314:1324	significant enhancement	1302:1324	significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells	1302:1409	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	1	24	theme	past	96:99	arg1	decades					101:107	the past decades	92:107	the past decades	92:107	In the past decades, extensive studies have demonstrated that carbon nanotubes (CNTs) could promote cell adhesion, proliferation and differentiation of neuronal cells.
28821138	5	25	theme	tensile	895:901	arg1	strength					903:910	tensile strength	895:910	tensile strength	895:910	The tensile strength and elongation at break of the Ch/CNT composite hydrogels were obviously enhanced, and the swelling ratio decreased.
28821138	0	26	theme	Biocompatible	0:12	arg1	chitin/carbon					14:26	Biocompatible chitin/carbon	0:26	Biocompatible chitin/carbon	0:26	Biocompatible chitin/carbon nanotubes composite hydrogels as neuronal growth substrates.
28821138	5	27	theme	swelling	1003:1010	arg1	ratio					1012:1016	the swelling ratio	999:1016	the swelling ratio	999:1016	The tensile strength and elongation at break of the Ch/CNT composite hydrogels were obviously enhanced, and the swelling ratio decreased.
28821138	4	28	theme	compact	723:729	arg1	network					759:765	a compact and neat Ch/CNT nanofibrous network	721:765	a compact and neat Ch/CNT nanofibrous network	721:765	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	6	29	theme	Schwann	1204:1210	arg1	cells					1212:1216	Schwann cells	1204:1216	Schwann cells (PC12 cells and RSC96 cells)	1204:1245	In addition, the Ch/CNT hydrogels exhibited good hemocompatibility, biodegradation in vitro and biocompatibility without cytotoxicity and neurotoxicity nature to neuronal and Schwann cells (PC12 cells and RSC96 cells).
28821138	4	30	theme	intermolecular	775:788	arg1	bonding					849:855	hydrogen bonding	840:855	hydrogen bonding	840:855	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	4	30	theme	intermolecular	775:788	arg1	interactions					826:837	electrostatic interactions	812:837	electrostatic interactions	812:837	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	4	30	theme	intermolecular	775:788	arg1	interactions					790:801	intermolecular interactions	775:801	intermolecular interactions	775:801	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	4	30	theme	intermolecular	775:788	arg1	interaction					873:883	amphiphilic interaction	861:883	amphiphilic interaction	861:883	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	1	31	theme	cell	189:192	arg1	adhesion					194:201	cell adhesion	189:201	cell adhesion	189:201	In the past decades, extensive studies have demonstrated that carbon nanotubes (CNTs) could promote cell adhesion, proliferation and differentiation of neuronal cells.
28821138	0	32	theme	composite	38:46	arg1	substrates					77:86	neuronal growth substrates	61:86	neuronal growth substrates	61:86	Biocompatible chitin/carbon nanotubes composite hydrogels as neuronal growth substrates.
28821138	0	32	theme	composite	38:46	arg1	hydrogels					48:56	composite hydrogels	38:56	composite hydrogels	38:56	Biocompatible chitin/carbon nanotubes composite hydrogels as neuronal growth substrates.
28821138	8	33	theme	neuronal	1581:1588	arg1	substrates					1597:1606	neuronal growth substrates	1581:1606	neuronal growth substrates for the potential application in nerve regeneration	1581:1658	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	8	33	theme	neuronal	1581:1588	arg1	hydrogels					1568:1576	the novel Ch/CNT hydrogels	1551:1576	the novel Ch/CNT hydrogels	1551:1576	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	5	34	theme	composite	950:958	arg1	hydrogels					960:968	the Ch/CNT composite hydrogels	939:968	the Ch/CNT composite hydrogels	939:968	The tensile strength and elongation at break of the Ch/CNT composite hydrogels were obviously enhanced, and the swelling ratio decreased.
28821138	3	35	theme	aqueous	561:567	arg1	system					569:574	11wt% NaOH/4wt% urea aqueous system	540:574	11wt% NaOH/4wt% urea aqueous system	540:574	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	7	36	from	increase	1424:1431	arg1	percentage					1445:1454	the percentage	1441:1454	the percentage	1441:1454	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	7	36	from	increase	1424:1431	arg1	length					1464:1469	the length	1460:1469	the length of neurites	1460:1481	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	6	37	theme	PC12	1219:1222	arg1	cells					1224:1228	PC12 cells	1219:1228	PC12 cells	1219:1228	In addition, the Ch/CNT hydrogels exhibited good hemocompatibility, biodegradation in vitro and biocompatibility without cytotoxicity and neurotoxicity nature to neuronal and Schwann cells (PC12 cells and RSC96 cells).
28821138	1	38	theme	extensive	110:118	arg1	studies					120:126	extensive studies	110:126	extensive studies	110:126	In the past decades, extensive studies have demonstrated that carbon nanotubes (CNTs) could promote cell adhesion, proliferation and differentiation of neuronal cells.
28821138	4	39	theme	chitin	695:700	arg1	nanofibers					702:711	chitin nanofibers	695:711	chitin nanofibers	695:711	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	3	40	theme	chitin/carbon	421:433	arg1	hydrogels					464:472	biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels	407:472	biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels	407:472	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	7	41	theme	neuronal	1333:1340	arg1	adhesion					1347:1354	the neuronal cell adhesion	1329:1354	the neuronal cell adhesion	1329:1354	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	4	42	theme	hydrogen	840:847	arg1	bonding					849:855	hydrogen bonding	840:855	hydrogen bonding	840:855	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	3	43	theme	Ch/CNT	446:451	arg1	hydrogels					464:472	biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels	407:472	biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels	407:472	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	7	44	theme	cell	1342:1345	arg1	adhesion					1347:1354	the neuronal cell adhesion	1329:1354	the neuronal cell adhesion	1329:1354	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	3	45	theme	nanotubes	435:443	arg1	hydrogels					464:472	biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels	407:472	biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels	407:472	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	8	46	theme	potential	1616:1624	arg1	application					1626:1636	the potential application	1612:1636	the potential application in nerve regeneration	1612:1658	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	5	47	dep	strength	903:910	arg1	The					891:893	The	891:893	The	891:893	The tensile strength and elongation at break of the Ch/CNT composite hydrogels were obviously enhanced, and the swelling ratio decreased.
28821138	7	48	theme	adhesion	1347:1354	arg1	enhancement					1314:1324	significant enhancement	1302:1324	significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells	1302:1409	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	0	49	theme	growth	70:75	arg1	substrates					77:86	neuronal growth substrates	61:86	neuronal growth substrates	61:86	Biocompatible chitin/carbon nanotubes composite hydrogels as neuronal growth substrates.
28821138	0	49	theme	growth	70:75	arg1	hydrogels					48:56	composite hydrogels	38:56	composite hydrogels	38:56	Biocompatible chitin/carbon nanotubes composite hydrogels as neuronal growth substrates.
28821138	6	50	dep	cytotoxicity	1150:1161	arg1	nature					1181:1186	nature	1181:1186	nature	1181:1186	In addition, the Ch/CNT hydrogels exhibited good hemocompatibility, biodegradation in vitro and biocompatibility without cytotoxicity and neurotoxicity nature to neuronal and Schwann cells (PC12 cells and RSC96 cells).
28821138	0	51	theme	neuronal	61:68	arg1	substrates					77:86	neuronal growth substrates	61:86	neuronal growth substrates	61:86	Biocompatible chitin/carbon nanotubes composite hydrogels as neuronal growth substrates.
28821138	0	51	theme	neuronal	61:68	arg1	hydrogels					48:56	composite hydrogels	38:56	composite hydrogels	38:56	Biocompatible chitin/carbon nanotubes composite hydrogels as neuronal growth substrates.
28821138	7	52	theme	neurite	1375:1381	arg1	outgrowth					1383:1391	neurite outgrowth	1375:1391	neurite outgrowth	1375:1391	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	8	53	theme	simple	1508:1513	arg1	approach					1529:1536	a simple and efficient approach	1506:1536	a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration	1506:1658	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	8	54	from	application	1626:1636	arg1	regeneration					1647:1658	nerve regeneration	1641:1658	nerve regeneration	1641:1658	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	2	55	theme	CNTs	354:357	arg1	utilization					339:349	the utilization	335:349	the utilization of CNTs as substrates for neural growth	335:389	However, the potential cytotoxicity in biological systems severely restricted the utilization of CNTs as substrates for neural growth.
28821138	2	56	from	cytotoxicity	280:291	arg1	systems					307:313	biological systems	296:313	biological systems	296:313	However, the potential cytotoxicity in biological systems severely restricted the utilization of CNTs as substrates for neural growth.
28821138	6	57	theme	RSC96	1234:1238	arg1	cells					1240:1244	RSC96 cells	1234:1244	RSC96 cells	1234:1244	In addition, the Ch/CNT hydrogels exhibited good hemocompatibility, biodegradation in vitro and biocompatibility without cytotoxicity and neurotoxicity nature to neuronal and Schwann cells (PC12 cells and RSC96 cells).
28821138	7	58	theme	cells	1405:1409	arg1	proliferation					1357:1369	proliferation	1357:1369	proliferation	1357:1369	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	7	58	theme	cells	1405:1409	arg1	outgrowth					1383:1391	neurite outgrowth	1375:1391	neurite outgrowth	1375:1391	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	7	58	theme	cells	1405:1409	arg1	adhesion					1347:1354	the neuronal cell adhesion	1329:1354	the neuronal cell adhesion	1329:1354	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	5	59	from	break	930:934	arg1	elongation					916:925	elongation	916:925	elongation	916:925	The tensile strength and elongation at break of the Ch/CNT composite hydrogels were obviously enhanced, and the swelling ratio decreased.
28821138	5	59	from	break	930:934	arg1	strength					903:910	tensile strength	895:910	tensile strength	895:910	The tensile strength and elongation at break of the Ch/CNT composite hydrogels were obviously enhanced, and the swelling ratio decreased.
28821138	5	60	theme	Ch/CNT	943:948	arg1	hydrogels					960:968	the Ch/CNT composite hydrogels	939:968	the Ch/CNT composite hydrogels	939:968	The tensile strength and elongation at break of the Ch/CNT composite hydrogels were obviously enhanced, and the swelling ratio decreased.
28821138	6	61	theme	Ch/CNT	1046:1051	arg1	hydrogels					1053:1061	the Ch/CNT hydrogels	1042:1061	the Ch/CNT hydrogels	1042:1061	In addition, the Ch/CNT hydrogels exhibited good hemocompatibility, biodegradation in vitro and biocompatibility without cytotoxicity and neurotoxicity nature to neuronal and Schwann cells (PC12 cells and RSC96 cells).
28821138	3	62	theme	NaOH/4wt	546:553	arg1	%					554:554	11wt% NaOH/4wt%	540:554	11wt% NaOH/4wt% urea aqueous system	540:574	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	8	63	theme	efficient	1519:1527	arg1	approach					1529:1536	a simple and efficient approach	1506:1536	a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration	1506:1658	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	3	64	theme	%	554:554	arg1	system					569:574	11wt% NaOH/4wt% urea aqueous system	540:574	11wt% NaOH/4wt% urea aqueous system	540:574	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	2	65	theme	neural	377:382	arg1	growth					384:389	neural growth	377:389	neural growth	377:389	However, the potential cytotoxicity in biological systems severely restricted the utilization of CNTs as substrates for neural growth.
28821138	8	66	theme	nerve	1641:1645	arg1	regeneration					1647:1658	nerve regeneration	1641:1658	nerve regeneration	1641:1658	Therefore, we provide a simple and efficient approach to construct the novel Ch/CNT hydrogels as neuronal growth substrates for the potential application in nerve regeneration.
28821138	7	67	theme	neuronal	1396:1403	arg1	cells					1405:1409	neuronal cells	1396:1409	neuronal cells	1396:1409	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	1	68	theme	carbon	151:156	arg1	nanotubes					158:166	carbon nanotubes	151:166	carbon nanotubes (CNTs)	151:173	In the past decades, extensive studies have demonstrated that carbon nanotubes (CNTs) could promote cell adhesion, proliferation and differentiation of neuronal cells.
28821138	1	68	theme	carbon	151:156	arg1	CNTs					169:172	CNTs	169:172	CNTs	169:172	In the past decades, extensive studies have demonstrated that carbon nanotubes (CNTs) could promote cell adhesion, proliferation and differentiation of neuronal cells.
28821138	4	69	theme	chitin	663:668	arg1	matrix					670:675	chitin matrix	663:675	chitin matrix	663:675	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	1	70	theme	neuronal	241:248	arg1	cells					250:254	neuronal cells	241:254	neuronal cells	241:254	In the past decades, extensive studies have demonstrated that carbon nanotubes (CNTs) could promote cell adhesion, proliferation and differentiation of neuronal cells.
28821138	7	71	theme	Ch/CNT3	1264:1270	arg1	hydrogels					1282:1290	the Ch/CNT3 composite hydrogels	1260:1290	the Ch/CNT3 composite hydrogels	1260:1290	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	4	72	theme	electrostatic	812:824	arg1	interactions					826:837	electrostatic interactions	812:837	electrostatic interactions	812:837	As the CNTs were dispersed homogeneously in chitin matrix and combined with chitin nanofibers to form a compact and neat Ch/CNT nanofibrous network through intermolecular interactions, such as electrostatic interactions, hydrogen bonding and amphiphilic interaction, etc.
28821138	3	73	theme	11wt	540:543	arg1	%					554:554	11wt% NaOH/4wt%	540:554	11wt% NaOH/4wt% urea aqueous system	540:574	In this study, biocompatible chitin/carbon nanotubes (Ch/CNT) composite hydrogels were developed via blending modified CNTs with chitin solution in 11wt% NaOH/4wt% urea aqueous system, and subsequently regenerating in ethanol.
28821138	7	74	theme	composite	1272:1280	arg1	hydrogels					1282:1290	the Ch/CNT3 composite hydrogels	1260:1290	the Ch/CNT3 composite hydrogels	1260:1290	Especially, the Ch/CNT3 composite hydrogels exhibited significant enhancement of the neuronal cell adhesion, proliferation and neurite outgrowth of neuronal cells with a great increase in both the percentage and the length of neurites.
28821138	1	75	theme	cells	250:254	arg1	differentiation					222:236	differentiation	222:236	differentiation	222:236	In the past decades, extensive studies have demonstrated that carbon nanotubes (CNTs) could promote cell adhesion, proliferation and differentiation of neuronal cells.
28821138	1	75	theme	cells	250:254	arg1	proliferation					204:216	proliferation	204:216	proliferation	204:216	In the past decades, extensive studies have demonstrated that carbon nanotubes (CNTs) could promote cell adhesion, proliferation and differentiation of neuronal cells.
28821138	1	75	theme	cells	250:254	arg1	adhesion					194:201	cell adhesion	189:201	cell adhesion	189:201	In the past decades, extensive studies have demonstrated that carbon nanotubes (CNTs) could promote cell adhesion, proliferation and differentiation of neuronal cells.
27083373	0	0	theme	growth	85:90	arg1	factor					92:97	vascular endothelial growth factor	64:97	vascular endothelial growth factor release	64:105	Facile method to prepare silk fibroin/hyaluronic acid films for vascular endothelial growth factor release.
27083373	4	1	theme	structural	641:650	arg1	integrity					652:660	good structural integrity	636:660	good structural integrity	636:660	After immersing in water for 24h, all of the films showed good structural integrity.
27083373	7	2	theme	drug	1035:1038	arg1	delivery					1040:1047	drug delivery	1035:1047	drug delivery	1035:1047	Overall, the 5% HA/SF films formed at 37°C with more rapid VEGF release exhibited great potential in drug delivery, especially when the rapid vascularization was needed.
27083373	1	3	theme	silk	150:153	arg1	SF					164:165	SF	164:165	SF	164:165	A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment.
27083373	1	3	theme	silk	150:153	arg1	fibroin					155:161	silk fibroin	150:161	silk fibroin (SF)	150:166	A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment.
27083373	1	4	theme	aqueous	214:220	arg1	solution					222:229	aqueous solution	214:229	aqueous solution	214:229	A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment.
27083373	0	5	theme	endothelial	73:83	arg1	factor					92:97	vascular endothelial growth factor	64:97	vascular endothelial growth factor release	64:105	Facile method to prepare silk fibroin/hyaluronic acid films for vascular endothelial growth factor release.
27083373	2	6	theme	film	314:317	arg1	formation					319:327	film formation	314:327	film formation	314:327	Only by controlling the HA content and film formation temperature during the film casting, the HA/SF films with different composition were prepared.
27083373	6	7	theme	rapid	853:857	arg1	release					859:865	the rapid release	849:865	the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films	849:931	Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films.
27083373	5	8	dep	formation	758:766	arg1	temperature					768:778	temperature	768:778	temperature	768:778	The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content.
27083373	0	9	theme	factor	92:97	arg1	release					99:105	vascular endothelial growth factor release	64:105	vascular endothelial growth factor release	64:105	Facile method to prepare silk fibroin/hyaluronic acid films for vascular endothelial growth factor release.
27083373	3	10	theme	water	520:524	arg1	characteristics					472:486	structural characteristics	461:486	structural characteristics	461:486	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	3	10	theme	water	520:524	arg1	stability					526:534	water stability	520:534	water stability	520:534	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	2	11	theme	formation	319:327	arg1	temperature					329:339	the HA content and film formation temperature	295:339	the HA content and film formation temperature during the film casting	295:363	Only by controlling the HA content and film formation temperature during the film casting, the HA/SF films with different composition were prepared.
27083373	6	12	theme	HA/SF	921:925	arg1	films					927:931	the HA/SF films	917:931	the HA/SF films	917:931	Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films.
27083373	5	13	theme	films	697:701	arg1	rate					679:682	The degradation rate	663:682	The degradation rate of the HA/SF films in protease XIV	663:717	The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content.
27083373	6	14	from	films	927:931	arg1	release					859:865	the rapid release	849:865	the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films	849:931	Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films.
27083373	7	15	with	37°C	972:975	arg1	release					998:1004	more rapid VEGF release	982:1004	more rapid VEGF release	982:1004	Overall, the 5% HA/SF films formed at 37°C with more rapid VEGF release exhibited great potential in drug delivery, especially when the rapid vascularization was needed.
27083373	5	16	from	rate	679:682	arg1	XIV					715:717	protease XIV	706:717	protease XIV	706:717	The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content.
27083373	7	17	theme	rapid	987:991	arg1	release					998:1004	more rapid VEGF release	982:1004	more rapid VEGF release	982:1004	Overall, the 5% HA/SF films formed at 37°C with more rapid VEGF release exhibited great potential in drug delivery, especially when the rapid vascularization was needed.
27083373	5	18	theme	film	753:756	arg1	formation					758:766	the film formation temperature and HA content	749:793	formation	758:766	The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content.
27083373	2	19	with	films	376:380	arg1	composition					397:407	different composition	387:407	different composition	387:407	Only by controlling the HA content and film formation temperature during the film casting, the HA/SF films with different composition were prepared.
27083373	7	20	theme	VEGF	993:996	arg1	release					998:1004	more rapid VEGF release	982:1004	more rapid VEGF release	982:1004	Overall, the 5% HA/SF films formed at 37°C with more rapid VEGF release exhibited great potential in drug delivery, especially when the rapid vascularization was needed.
27083373	5	21	theme	protease	706:713	arg1	XIV					715:717	protease XIV	706:717	protease XIV	706:717	The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content.
27083373	3	22	theme	water	537:541	arg1	characteristics					472:486	structural characteristics	461:486	structural characteristics	461:486	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	3	22	theme	water	537:541	arg1	absorption					543:552	water absorption	537:552	water absorption	537:552	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	2	23	theme	film	352:355	arg1	casting					357:363	the film casting	348:363	the film casting	348:363	Only by controlling the HA content and film formation temperature during the film casting, the HA/SF films with different composition were prepared.
27083373	0	24	theme	Facile	0:5	arg1	method					7:12	Facile method	0:12	Facile method	0:12	Facile method to prepare silk fibroin/hyaluronic acid films for vascular endothelial growth factor release.
27083373	7	25	theme	rapid	1070:1074	arg1	vascularization					1076:1090	the rapid vascularization	1066:1090	the rapid vascularization	1066:1090	Overall, the 5% HA/SF films formed at 37°C with more rapid VEGF release exhibited great potential in drug delivery, especially when the rapid vascularization was needed.
27083373	7	26	theme	great	1016:1020	arg1	potential					1022:1030	great potential	1016:1030	great potential	1016:1030	Overall, the 5% HA/SF films formed at 37°C with more rapid VEGF release exhibited great potential in drug delivery, especially when the rapid vascularization was needed.
27083373	1	27	theme	hyaluronic	172:181	arg1	acid					183:186	hyaluronic acid	172:186	hyaluronic acid (HA)	172:191	A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment.
27083373	1	27	theme	hyaluronic	172:181	arg1	HA					189:190	HA	189:190	HA	189:190	A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment.
27083373	2	28	theme	different	387:395	arg1	composition					397:407	different composition	387:407	different composition	387:407	Only by controlling the HA content and film formation temperature during the film casting, the HA/SF films with different composition were prepared.
27083373	1	29	theme	post	259:262	arg1	treatment					264:272	any post treatment	255:272	any post treatment	255:272	A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment.
27083373	5	30	theme	degradation	667:677	arg1	rate					679:682	The degradation rate	663:682	The degradation rate of the HA/SF films in protease XIV	663:717	The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content.
27083373	5	31	from	films	697:701	arg1	XIV					715:717	protease XIV	706:717	protease XIV	706:717	The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content.
27083373	1	32	theme	fibroin	155:161	arg1	films					203:207	silk fibroin (SF) and hyaluronic acid (HA) composite films	150:207	silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution	150:229	A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment.
27083373	1	33	theme	acid	183:186	arg1	films					203:207	silk fibroin (SF) and hyaluronic acid (HA) composite films	150:207	silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution	150:229	A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment.
27083373	0	34	theme	fibroin/hyaluronic	30:47	arg1	films					54:58	silk fibroin/hyaluronic acid films	25:58	silk fibroin/hyaluronic acid films for vascular endothelial growth factor release	25:105	Facile method to prepare silk fibroin/hyaluronic acid films for vascular endothelial growth factor release.
27083373	3	35	theme	structural	461:470	arg1	properties					566:575	mechanical properties	555:575	mechanical properties	555:575	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	3	35	theme	structural	461:470	arg1	stability					526:534	water stability	520:534	water stability	520:534	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	3	35	theme	structural	461:470	arg1	stability					497:505	thermal stability	489:505	thermal stability	489:505	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	3	35	theme	structural	461:470	arg1	absorption					543:552	water absorption	537:552	water absorption	537:552	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	3	35	theme	structural	461:470	arg1	morphology					508:517	morphology	508:517	morphology	508:517	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	3	35	theme	structural	461:470	arg1	characteristics					472:486	structural characteristics	461:486	structural characteristics	461:486	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	2	36	theme	HA/SF	370:374	arg1	films					376:380	the HA/SF films	366:380	the HA/SF films with different composition	366:407	Only by controlling the HA content and film formation temperature during the film casting, the HA/SF films with different composition were prepared.
27083373	0	37	theme	silk	25:28	arg1	films					54:58	silk fibroin/hyaluronic acid films	25:58	silk fibroin/hyaluronic acid films for vascular endothelial growth factor release	25:105	Facile method to prepare silk fibroin/hyaluronic acid films for vascular endothelial growth factor release.
27083373	6	38	theme	growth	897:902	arg1	factor					904:909	vascular endothelial growth factor	876:909	vascular endothelial growth factor	876:909	Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films.
27083373	6	38	theme	growth	897:902	arg1	VEGF					870:873	VEGF	870:873	VEGF (vascular endothelial growth factor)	870:910	Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films.
27083373	1	39	from	solution	222:229	arg1	films					203:207	silk fibroin (SF) and hyaluronic acid (HA) composite films	150:207	silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution	150:229	A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment.
27083373	7	40	theme	5	947:947	arg1	%					948:948	%	948:948	%	948:948	Overall, the 5% HA/SF films formed at 37°C with more rapid VEGF release exhibited great potential in drug delivery, especially when the rapid vascularization was needed.
27083373	6	41	theme	endothelial	885:895	arg1	factor					904:909	vascular endothelial growth factor	876:909	vascular endothelial growth factor	876:909	Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films.
27083373	6	41	theme	endothelial	885:895	arg1	VEGF					870:873	VEGF	870:873	VEGF (vascular endothelial growth factor)	870:910	Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films.
27083373	7	42	theme	%	948:948	arg1	films					956:960	the 5% HA/SF films	943:960	the 5% HA/SF films formed at 37°C with more rapid VEGF release	943:1004	Overall, the 5% HA/SF films formed at 37°C with more rapid VEGF release exhibited great potential in drug delivery, especially when the rapid vascularization was needed.
27083373	1	43	theme	facile	110:115	arg1	approach					117:124	A facile approach	108:124	A facile approach	108:124	A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment.
27083373	0	44	theme	acid	49:52	arg1	films					54:58	silk fibroin/hyaluronic acid films	25:58	silk fibroin/hyaluronic acid films for vascular endothelial growth factor release	25:105	Facile method to prepare silk fibroin/hyaluronic acid films for vascular endothelial growth factor release.
27083373	6	45	theme	vascular	876:883	arg1	factor					904:909	vascular endothelial growth factor	876:909	vascular endothelial growth factor	876:909	Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films.
27083373	6	45	theme	vascular	876:883	arg1	VEGF					870:873	VEGF	870:873	VEGF (vascular endothelial growth factor)	870:910	Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films.
27083373	3	46	theme	mechanical	555:564	arg1	properties					566:575	mechanical properties	555:575	mechanical properties	555:575	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	3	46	theme	mechanical	555:564	arg1	characteristics					472:486	structural characteristics	461:486	structural characteristics	461:486	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	2	47	theme	content	302:308	arg1	temperature					329:339	the HA content and film formation temperature	295:339	the HA content and film formation temperature during the film casting	295:363	Only by controlling the HA content and film formation temperature during the film casting, the HA/SF films with different composition were prepared.
27083373	7	48	theme	HA/SF	950:954	arg1	films					956:960	the 5% HA/SF films	943:960	the 5% HA/SF films formed at 37°C with more rapid VEGF release	943:1004	Overall, the 5% HA/SF films formed at 37°C with more rapid VEGF release exhibited great potential in drug delivery, especially when the rapid vascularization was needed.
27083373	2	49	theme	HA	299:300	arg1	content					302:308	HA content	299:308	HA content	299:308	Only by controlling the HA content and film formation temperature during the film casting, the HA/SF films with different composition were prepared.
27083373	1	50	theme	composite	193:201	arg1	films					203:207	silk fibroin (SF) and hyaluronic acid (HA) composite films	150:207	silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution	150:229	A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment.
27083373	0	51	theme	vascular	64:71	arg1	factor					92:97	vascular endothelial growth factor	64:97	vascular endothelial growth factor release	64:105	Facile method to prepare silk fibroin/hyaluronic acid films for vascular endothelial growth factor release.
27083373	5	52	from	XIV	715:717	arg1	rate					679:682	The degradation rate	663:682	The degradation rate of the HA/SF films in protease XIV	663:717	The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content.
27083373	5	53	theme	HA	784:785	arg1	content					787:793	HA content	784:793	HA content	784:793	The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content.
27083373	3	54	theme	thermal	489:495	arg1	characteristics					472:486	structural characteristics	461:486	structural characteristics	461:486	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	3	54	theme	thermal	489:495	arg1	stability					497:505	thermal stability	489:505	thermal stability	489:505	The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties.
27083373	6	55	theme	VEGF	870:873	arg1	release					859:865	the rapid release	849:865	the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films	849:931	Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films.
27083373	4	56	theme	good	636:639	arg1	integrity					652:660	good structural integrity	636:660	good structural integrity	636:660	After immersing in water for 24h, all of the films showed good structural integrity.
27083373	5	57	theme	HA/SF	691:695	arg1	films					697:701	the HA/SF films	687:701	the HA/SF films in protease XIV	687:717	The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content.
26407208	11	0	theme	CS-based	1694:1701	arg1	NPs					1703:1705	CS-based NPs	1694:1705	CS-based NPs	1694:1705	These results demonstrate the potential of CS-based NPs for the ocular delivery of KT.
26407208	8	1	theme	formulated	1345:1354	arg1	NPs					1356:1358	the formulated NPs	1341:1358	the formulated NPs	1341:1358	Furthermore, mucoadhesion studies revealed adhesive properties of the formulated NPs.
26407208	1	2	theme	-based	143:148	arg1	NPs					165:167	NPs	165:167	NPs	165:167	The aim of this work was to formulate chitosan (CS)-based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery.
26407208	1	2	theme	-based	143:148	arg1	nanoparticles					150:162	chitosan (CS)-based nanoparticles	130:162	chitosan (CS)-based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery	130:245	The aim of this work was to formulate chitosan (CS)-based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery.
26407208	10	3	from	drug	1597:1600	arg1	surface					1613:1619	the eye surface	1605:1619	the eye surface	1605:1619	The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time.
26407208	1	4	theme	topical	223:229	arg1	delivery					238:245	topical ocular delivery	223:245	topical ocular delivery	223:245	The aim of this work was to formulate chitosan (CS)-based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery.
26407208	10	5	theme	corneal	1485:1491	arg1	studies					1504:1510	The ex vivo corneal permeation studies	1473:1510	The ex vivo corneal permeation studies performed on excised porcine eye balls	1473:1549	The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time.
26407208	6	6	theme	CS	1157:1158	arg1	CS					1157:1158	CS	1157:1158	CS	1157:1158	FT-IR and DSC revealed that KT was dispersed within the NPs where the phosphate groups of TPP were associated with the ammonium groups of CS.
26407208	6	6	theme	CS	1157:1158	arg1	groups					1147:1152	the ammonium groups	1134:1152	the ammonium groups of CS	1134:1158	FT-IR and DSC revealed that KT was dispersed within the NPs where the phosphate groups of TPP were associated with the ammonium groups of CS.
26407208	3	7	theme	efficiency	480:489	arg1	terms					423:427	terms	423:427	terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC)	423:666	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	1	8	theme	ocular	231:236	arg1	delivery					238:245	topical ocular delivery	223:245	topical ocular delivery	223:245	The aim of this work was to formulate chitosan (CS)-based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery.
26407208	7	9	dep	in	1165:1166	arg1	vitro					1168:1172	vitro	1168:1172	vitro	1168:1172	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	3	10	theme	morphology	500:509	arg1	terms					423:427	terms	423:427	terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC)	423:666	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	5	11	theme	drug	871:874	arg1	concentration					876:888	the corresponding drug concentration	853:888	the corresponding drug concentration	853:888	A correlation between drug EE and the corresponding drug concentration added to the formulation was observed, where the EE of the NPs increased with increasing drug concentration, for up to 10 mg/mL.
26407208	11	12	theme	KT	1734:1735	arg1	delivery					1722:1729	the ocular delivery	1711:1729	the ocular delivery of KT	1711:1735	These results demonstrate the potential of CS-based NPs for the ocular delivery of KT.
26407208	10	13	theme	eye	1609:1611	arg1	surface					1613:1619	the eye surface	1605:1619	the eye surface	1605:1619	The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time.
26407208	9	14	theme	storage	1428:1434	arg1	conditions					1436:1445	different storage conditions	1418:1445	different storage conditions	1418:1445	The KT-loaded NPs were found to be stable when stored at different storage conditions for a period of 3 months.
26407208	4	15	theme	257.2	802:806	arg1	nm					796:797	nm	796:797	nm	796:797	The data suggested that the size of the NPs was affected by CS/TPP ratio where the diameter of the NPs ranged from 108.0 ± 2.4 nm to 257.2 ± 18.6 nm.
26407208	3	16	theme	entrapment	469:478	arg1	efficiency					480:489	entrapment efficiency	469:489	entrapment efficiency (EE)	469:494	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	3	16	theme	entrapment	469:478	arg1	EE					492:493	EE	492:493	EE	492:493	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	10	17	theme	excised	1525:1531	arg1	balls					1545:1549	excised porcine eye balls	1525:1549	excised porcine eye balls	1525:1549	The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time.
26407208	6	18	theme	ammonium	1138:1145	arg1	CS					1157:1158	CS	1157:1158	CS	1157:1158	FT-IR and DSC revealed that KT was dispersed within the NPs where the phosphate groups of TPP were associated with the ammonium groups of CS.
26407208	6	18	theme	ammonium	1138:1145	arg1	groups					1147:1152	the ammonium groups	1134:1152	the ammonium groups of CS	1134:1158	FT-IR and DSC revealed that KT was dispersed within the NPs where the phosphate groups of TPP were associated with the ammonium groups of CS.
26407208	3	19	theme	size	447:450	arg1	terms					423:427	terms	423:427	terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC)	423:666	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	4	20	theme	CS/TPP	729:734	arg1	ratio					736:740	CS/TPP ratio	729:740	CS/TPP ratio where the diameter of the NPs ranged from 108.0 ± 2.4 nm to 257.2 ± 18.6 nm	729:816	The data suggested that the size of the NPs was affected by CS/TPP ratio where the diameter of the NPs ranged from 108.0 ± 2.4 nm to 257.2 ± 18.6 nm.
26407208	7	21	from	NPs	1204:1206	arg1	KT					1193:1194	KT	1193:1194	KT from CS NPs	1193:1206	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	7	21	from	NPs	1204:1206	arg1	profile					1182:1188	The in vitro release profile	1161:1188	The in vitro release profile of KT from CS NPs	1161:1206	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	7	22	theme	KT	1193:1194	arg1	profile					1182:1188	The in vitro release profile	1161:1188	The in vitro release profile of KT from CS NPs	1161:1206	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	4	23	dep	nm	796:797	arg1	to					799:800	to	799:800	to	799:800	The data suggested that the size of the NPs was affected by CS/TPP ratio where the diameter of the NPs ranged from 108.0 ± 2.4 nm to 257.2 ± 18.6 nm.
26407208	10	24	theme	porcine	1533:1539	arg1	balls					1545:1549	excised porcine eye balls	1525:1549	excised porcine eye balls	1525:1549	The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time.
26407208	7	25	theme	release	1174:1180	arg1	profile					1182:1188	The in vitro release profile	1161:1188	The in vitro release profile of KT from CS NPs	1161:1206	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	10	26	theme	NPs	1576:1578	arg1	ability					1565:1571	the ability	1561:1571	the ability of NPs	1561:1578	The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time.
26407208	8	27	theme	adhesive	1318:1325	arg1	properties					1327:1336	adhesive properties	1318:1336	adhesive properties of the formulated NPs	1318:1358	Furthermore, mucoadhesion studies revealed adhesive properties of the formulated NPs.
26407208	4	28	theme	2.4	792:794	arg1	nm					796:797	nm	796:797	nm	796:797	The data suggested that the size of the NPs was affected by CS/TPP ratio where the diameter of the NPs ranged from 108.0 ± 2.4 nm to 257.2 ± 18.6 nm.
26407208	5	29	theme	corresponding	857:869	arg1	concentration					876:888	the corresponding drug concentration	853:888	the corresponding drug concentration	853:888	A correlation between drug EE and the corresponding drug concentration added to the formulation was observed, where the EE of the NPs increased with increasing drug concentration, for up to 10 mg/mL.
26407208	10	30	theme	ex	1477:1478	arg1	studies					1504:1510	The ex vivo corneal permeation studies	1473:1510	The ex vivo corneal permeation studies performed on excised porcine eye balls	1473:1549	The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time.
26407208	10	31	dep	ex	1477:1478	arg1	vivo					1480:1483	vivo	1480:1483	vivo	1480:1483	The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time.
26407208	10	32	theme	permeation	1493:1502	arg1	studies					1504:1510	The ex vivo corneal permeation studies	1473:1510	The ex vivo corneal permeation studies performed on excised porcine eye balls	1473:1549	The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time.
26407208	11	33	theme	ocular	1715:1720	arg1	delivery					1722:1729	the ocular delivery	1711:1729	the ocular delivery of KT	1711:1735	These results demonstrate the potential of CS-based NPs for the ocular delivery of KT.
26407208	9	34	theme	months	1465:1470	arg1	period					1453:1458	a period	1451:1458	a period of 3 months	1451:1470	The KT-loaded NPs were found to be stable when stored at different storage conditions for a period of 3 months.
26407208	4	35	theme	NPs	768:770	arg1	diameter					752:759	the diameter	748:759	the diameter of the NPs ranged from 108.0 ± 2.4 nm to 257.2	748:806	The data suggested that the size of the NPs was affected by CS/TPP ratio where the diameter of the NPs ranged from 108.0 ± 2.4 nm to 257.2 ± 18.6 nm.
26407208	2	36	theme	gelation	278:285	arg1	method					287:292	ionic gelation method	272:292	ionic gelation method incorporating tri-polyphosphate (TPP) as cross-linker	272:346	NPs were prepared using ionic gelation method incorporating tri-polyphosphate (TPP) as cross-linker.
26407208	3	37	theme	particle	438:445	arg1	size					447:450	their particle size	432:450	their particle size	432:450	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	0	38	theme	corneal	16:22	arg1	permeation					24:33	corneal permeation	16:33	corneal permeation	16:33	Formulation and corneal permeation of ketorolac tromethamine-loaded chitosan nanoparticles.
26407208	7	39	theme	KT	1262:1263	arg1	solution					1265:1272	KT solution	1262:1272	KT solution	1262:1272	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	8	40	theme	mucoadhesion	1288:1299	arg1	studies					1301:1307	mucoadhesion studies	1288:1307	mucoadhesion studies	1288:1307	Furthermore, mucoadhesion studies revealed adhesive properties of the formulated NPs.
26407208	2	41	theme	ionic	272:276	arg1	method					287:292	ionic gelation method	272:292	ionic gelation method incorporating tri-polyphosphate (TPP) as cross-linker	272:346	NPs were prepared using ionic gelation method incorporating tri-polyphosphate (TPP) as cross-linker.
26407208	1	42	theme	ketorolac	182:190	arg1	KT					206:207	KT	206:207	KT	206:207	The aim of this work was to formulate chitosan (CS)-based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery.
26407208	1	42	theme	ketorolac	182:190	arg1	tromethamine					192:203	ketorolac tromethamine	182:203	ketorolac tromethamine (KT) intended for topical ocular delivery	182:245	The aim of this work was to formulate chitosan (CS)-based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery.
26407208	0	43	theme	ketorolac	38:46	arg1	chitosan					68:75	ketorolac tromethamine-loaded chitosan	38:75	ketorolac tromethamine-loaded chitosan	38:75	Formulation and corneal permeation of ketorolac tromethamine-loaded chitosan nanoparticles.
26407208	7	44	theme	in	1165:1166	arg1	profile					1182:1188	The in vitro release profile	1161:1188	The in vitro release profile of KT from CS NPs	1161:1206	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	5	45	theme	drug	979:982	arg1	concentration					984:996	drug concentration	979:996	drug concentration	979:996	A correlation between drug EE and the corresponding drug concentration added to the formulation was observed, where the EE of the NPs increased with increasing drug concentration, for up to 10 mg/mL.
26407208	7	46	theme	p	1240:1240	arg1	differences					1227:1237	significant differences	1215:1237	significant differences (p < 0.05)	1215:1248	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	7	46	theme	p	1240:1240	arg1	<					1242:1242	p < 0.05	1240:1247	p < 0.05	1240:1247	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	11	47	theme	NPs	1703:1705	arg1	potential					1681:1689	the potential	1677:1689	the potential of CS-based NPs for the ocular delivery of KT	1677:1735	These results demonstrate the potential of CS-based NPs for the ocular delivery of KT.
26407208	3	48	theme	system	399:404	arg1	composition					380:390	the composition	376:390	the composition of the system	376:404	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	6	49	theme	TPP	1109:1111	arg1	TPP					1109:1111	TPP	1109:1111	TPP	1109:1111	FT-IR and DSC revealed that KT was dispersed within the NPs where the phosphate groups of TPP were associated with the ammonium groups of CS.
26407208	6	49	theme	TPP	1109:1111	arg1	groups					1099:1104	the phosphate groups	1085:1104	the phosphate groups of TPP	1085:1111	FT-IR and DSC revealed that KT was dispersed within the NPs where the phosphate groups of TPP were associated with the ammonium groups of CS.
26407208	4	50	theme	NPs	709:711	arg1	size					697:700	the size	693:700	the size of the NPs	693:711	The data suggested that the size of the NPs was affected by CS/TPP ratio where the diameter of the NPs ranged from 108.0 ± 2.4 nm to 257.2 ± 18.6 nm.
26407208	0	51	theme	chitosan	68:75	arg1	Formulation					0:10	Formulation	0:10	Formulation	0:10	Formulation and corneal permeation of ketorolac tromethamine-loaded chitosan nanoparticles.
26407208	0	51	theme	chitosan	68:75	arg1	permeation					24:33	corneal permeation	16:33	corneal permeation	16:33	Formulation and corneal permeation of ketorolac tromethamine-loaded chitosan nanoparticles.
26407208	5	52	dep	10	1009:1010	arg1	to					1006:1007	to	1006:1007	to	1006:1007	A correlation between drug EE and the corresponding drug concentration added to the formulation was observed, where the EE of the NPs increased with increasing drug concentration, for up to 10 mg/mL.
26407208	3	53	theme	structural	534:543	arg1	studies					562:568	structural characterization studies	534:568	structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC)	534:666	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	3	54	theme	potential	458:466	arg1	terms					423:427	terms	423:427	terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC)	423:666	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	0	55	theme	tromethamine-loaded	48:66	arg1	chitosan					68:75	ketorolac tromethamine-loaded chitosan	38:75	ketorolac tromethamine-loaded chitosan	38:75	Formulation and corneal permeation of ketorolac tromethamine-loaded chitosan nanoparticles.
26407208	3	56	theme	characterization	545:560	arg1	studies					562:568	structural characterization studies	534:568	structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC)	534:666	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	10	57	theme	eye	1541:1543	arg1	balls					1545:1549	excised porcine eye balls	1525:1549	excised porcine eye balls	1525:1549	The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time.
26407208	10	58	theme	longer	1638:1643	arg1	time					1645:1648	a relatively longer time	1625:1648	a relatively longer time	1625:1648	The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time.
26407208	5	59	theme	NPs	949:951	arg1	EE					939:940	the EE	935:940	the EE of the NPs	935:951	A correlation between drug EE and the corresponding drug concentration added to the formulation was observed, where the EE of the NPs increased with increasing drug concentration, for up to 10 mg/mL.
26407208	3	60	theme	infrared	594:601	arg1	FT-IR					617:621	FT-IR	617:621	FT-IR	617:621	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	3	60	theme	infrared	594:601	arg1	spectroscopy					603:614	infrared spectroscopy	594:614	infrared spectroscopy (FT-IR)	594:622	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	1	61	theme	chitosan	130:137	arg1	NPs					165:167	NPs	165:167	NPs	165:167	The aim of this work was to formulate chitosan (CS)-based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery.
26407208	1	61	theme	chitosan	130:137	arg1	nanoparticles					150:162	chitosan (CS)-based nanoparticles	130:162	chitosan (CS)-based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery	130:245	The aim of this work was to formulate chitosan (CS)-based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery.
26407208	1	62	theme	work	108:111	arg1	aim					96:98	The aim	92:98	The aim of this work	92:111	The aim of this work was to formulate chitosan (CS)-based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery.
26407208	3	63	theme	differential	628:639	arg1	DSC					663:665	DSC	663:665	DSC	663:665	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	3	63	theme	differential	628:639	arg1	calorimetry					650:660	differential scanning calorimetry	628:660	differential scanning calorimetry (DSC)	628:666	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	7	64	theme	significant	1215:1225	arg1	differences					1227:1237	significant differences	1215:1237	significant differences (p < 0.05)	1215:1248	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	7	64	theme	significant	1215:1225	arg1	<					1242:1242	p < 0.05	1240:1247	p < 0.05	1240:1247	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	7	65	from	profile	1182:1188	arg1	NPs					1204:1206	CS NPs	1201:1206	CS NPs	1201:1206	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	3	66	theme	zeta	453:456	arg1	potential					458:466	zeta potential	453:466	zeta potential	453:466	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	5	67	theme	drug	841:844	arg1	EE					846:847	drug EE	841:847	drug EE	841:847	A correlation between drug EE and the corresponding drug concentration added to the formulation was observed, where the EE of the NPs increased with increasing drug concentration, for up to 10 mg/mL.
26407208	3	68	theme	scanning	641:648	arg1	DSC					663:665	DSC	663:665	DSC	663:665	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	3	68	theme	scanning	641:648	arg1	calorimetry					650:660	differential scanning calorimetry	628:660	differential scanning calorimetry (DSC)	628:666	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	7	69	theme	CS	1201:1202	arg1	NPs					1204:1206	CS NPs	1201:1206	CS NPs	1201:1206	The in vitro release profile of KT from CS NPs showed significant differences (p < 0.05) compared to KT solution.
26407208	3	70	dep	Fourier	576:582	arg1	transform					584:592	transform	584:592	transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC)	584:666	Following the preparation, the composition of the system was optimized in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterization studies using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).
26407208	9	71	theme	KT-loaded	1365:1373	arg1	stable					1396:1401	stable	1396:1401	stable	1396:1401	The KT-loaded NPs were found to be stable when stored at different storage conditions for a period of 3 months.
26407208	9	71	theme	KT-loaded	1365:1373	arg1	NPs					1375:1377	The KT-loaded NPs	1361:1377	The KT-loaded NPs	1361:1377	The KT-loaded NPs were found to be stable when stored at different storage conditions for a period of 3 months.
26407208	8	72	theme	NPs	1356:1358	arg1	properties					1327:1336	adhesive properties	1318:1336	adhesive properties of the formulated NPs	1318:1358	Furthermore, mucoadhesion studies revealed adhesive properties of the formulated NPs.
26407208	9	73	theme	different	1418:1426	arg1	conditions					1436:1445	different storage conditions	1418:1445	different storage conditions	1418:1445	The KT-loaded NPs were found to be stable when stored at different storage conditions for a period of 3 months.
26407208	6	74	theme	phosphate	1089:1097	arg1	TPP					1109:1111	TPP	1109:1111	TPP	1109:1111	FT-IR and DSC revealed that KT was dispersed within the NPs where the phosphate groups of TPP were associated with the ammonium groups of CS.
26407208	6	74	theme	phosphate	1089:1097	arg1	groups					1099:1104	the phosphate groups	1085:1104	the phosphate groups of TPP	1085:1111	FT-IR and DSC revealed that KT was dispersed within the NPs where the phosphate groups of TPP were associated with the ammonium groups of CS.
28388835	13	0	theme	composite	1451:1459	arg1	events					1470:1475	composite endpoint events	1451:1475	composite endpoint events in either HIT group	1451:1495	Non-heparin anticoagulation was not associated with a reduction in composite endpoint events in either HIT group.
28388835	0	1	from	outcomes	9:16	arg1	cohort					23:28	a cohort	21:28	a cohort of patients with heparin-induced thrombocytopenia	21:78	Clinical outcomes in a cohort of patients with heparin-induced thrombocytopenia.
28388835	1	2	with	treatment	174:182	arg1	anticoagulants					201:214	non-heparin anticoagulants	189:214	non-heparin anticoagulants	189:214	BACKGROUND Heparin-induced thrombocytopenia (HIT) is a thrombotic disorder usually prompting treatment with non-heparin anticoagulants.
28388835	10	3	dep	2.48	1127:1130	arg1	P = .003					1145:1152	P = .003	1145:1152	P = .003	1145:1152	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	15	4	from	thrombosis	1749:1758	arg1	patients					1787:1794	individual patients	1776:1794	individual patients	1776:1794	Non-heparin anticoagulant treatment may not benefit all patients and should be considered only after careful assessment of the relative risks of thrombosis and bleeding in individual patients.
28388835	3	5	theme	antibody	375:382	arg1	test					384:387	a positive heparin-platelet factor 4 antibody test	338:387	a positive heparin-platelet factor 4 antibody test	338:387	METHODS We analyzed data for 442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure.
28388835	7	6	located	found	777:781	arg2	score					767:771	An intermediate or high "4T" score	738:771	An intermediate or high "4T" score	738:771	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	7	6	located	found	777:781	arg1	groups					827:832	the three respective groups	806:832	the three respective groups	806:832	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	7	6	located	found	777:781	arg1	%					801:801	8%	800:801	8%	800:801	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	7	6	located	found	777:781	arg1	%					788:788	85%	786:788	85%	786:788	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	7	6	located	found	777:781	arg1	%					793:793	58%	791:793	58%	791:793	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	7	7	theme	respective	816:825	arg1	groups					827:832	the three respective groups	806:832	the three respective groups	806:832	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	14	8	theme	HIT	1513:1515	arg1	patients					1517:1524	INTERPRETATION HIT patients	1498:1524	INTERPRETATION HIT patients	1498:1524	INTERPRETATION HIT patients have high risks of death, limb amputation/gangrene, thrombosis, and bleeding.
28388835	14	9	contain	have	1526:1529	arg1	patients					1517:1524	INTERPRETATION HIT patients	1498:1524	INTERPRETATION HIT patients	1498:1524	INTERPRETATION HIT patients have high risks of death, limb amputation/gangrene, thrombosis, and bleeding.
28388835	14	9	contain	have	1526:1529	arg2	risks					1536:1540	high risks	1531:1540	high risks of death	1531:1549	INTERPRETATION HIT patients have high risks of death, limb amputation/gangrene, thrombosis, and bleeding.
28388835	10	10	theme	HIT-T	1117:1121	arg1	1.35-4.55					1133:1141	1.35-4.55	1133:1141	1.35-4.55	1133:1141	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	10	10	theme	HIT-T	1117:1121	arg1	2.48					1127:1130	HIT-T [HR 2.48	1117:1130	HIT-T [HR 2.48 (1.35-4.55), P = .003)]	1117:1154	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	7	11	theme	"	765:765	arg1	score					767:771	An intermediate or high "4T" score	738:771	An intermediate or high "4T" score	738:771	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	7	12	theme	4T	763:764	arg1	score					767:771	An intermediate or high "4T" score	738:771	An intermediate or high "4T" score	738:771	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	3	13	theme	factor	366:371	arg1	test					384:387	a positive heparin-platelet factor 4 antibody test	338:387	a positive heparin-platelet factor 4 antibody test	338:387	METHODS We analyzed data for 442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure.
28388835	13	14	theme	HIT	1487:1489	arg1	group					1491:1495	either HIT group	1480:1495	either HIT group	1480:1495	Non-heparin anticoagulation was not associated with a reduction in composite endpoint events in either HIT group.
28388835	4	15	theme	primary	422:428	arg1	endpoint					454:461	a composite endpoint	442:461	a composite endpoint (death, limb amputation/gangrene, or new thrombosis)	442:514	The primary outcome was a composite endpoint (death, limb amputation/gangrene, or new thrombosis).
28388835	4	15	theme	primary	422:428	arg1	outcome					430:436	The primary outcome	418:436	The primary outcome	418:436	The primary outcome was a composite endpoint (death, limb amputation/gangrene, or new thrombosis).
28388835	10	16	theme	HIT	1050:1052	arg1	group					1054:1058	the no HIT group	1043:1058	the no HIT group	1043:1058	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	14	17	theme	INTERPRETATION	1498:1511	arg1	patients					1517:1524	INTERPRETATION HIT patients	1498:1524	INTERPRETATION HIT patients	1498:1524	INTERPRETATION HIT patients have high risks of death, limb amputation/gangrene, thrombosis, and bleeding.
28388835	4	18	theme	new	500:502	arg1	thrombosis					504:513	new thrombosis	500:513	new thrombosis	500:513	The primary outcome was a composite endpoint (death, limb amputation/gangrene, or new thrombosis).
28388835	12	19	theme	groups	1365:1370	arg1	%					1350:1350	16%	1348:1350	16%	1348:1350	Major bleeding occurred in 48%, 36%, and 16% of the three groups (P = .005).
28388835	12	19	theme	groups	1365:1370	arg1	P = .005					1373:1380	P = .005	1373:1380	P = .005	1373:1380	Major bleeding occurred in 48%, 36%, and 16% of the three groups (P = .005).
28388835	12	19	theme	groups	1365:1370	arg1	%					1336:1336	48%	1334:1336	48%	1334:1336	Major bleeding occurred in 48%, 36%, and 16% of the three groups (P = .005).
28388835	12	19	theme	groups	1365:1370	arg1	%					1341:1341	36%	1339:1341	36%	1339:1341	Major bleeding occurred in 48%, 36%, and 16% of the three groups (P = .005).
28388835	12	19	theme	groups	1365:1370	arg1	groups					1365:1370	the three groups	1355:1370	the three groups (P = .005)	1355:1381	Major bleeding occurred in 48%, 36%, and 16% of the three groups (P = .005).
28388835	1	20	theme	BACKGROUND	81:90	arg1	thrombocytopenia					108:123	BACKGROUND Heparin-induced thrombocytopenia	81:123	BACKGROUND Heparin-induced thrombocytopenia (HIT)	81:129	BACKGROUND Heparin-induced thrombocytopenia (HIT) is a thrombotic disorder usually prompting treatment with non-heparin anticoagulants.
28388835	1	20	theme	BACKGROUND	81:90	arg1	disorder					147:154	a thrombotic disorder	134:154	a thrombotic disorder usually prompting treatment with non-heparin anticoagulants	134:214	BACKGROUND Heparin-induced thrombocytopenia (HIT) is a thrombotic disorder usually prompting treatment with non-heparin anticoagulants.
28388835	1	20	theme	BACKGROUND	81:90	arg1	HIT					126:128	HIT	126:128	HIT	126:128	BACKGROUND Heparin-induced thrombocytopenia (HIT) is a thrombotic disorder usually prompting treatment with non-heparin anticoagulants.
28388835	9	21	theme	composite	899:907	arg1	endpoint					909:916	The composite endpoint	895:916	The composite endpoint	895:916	The composite endpoint occurred in 48%, 36%, and 17% (P = .01) of which 61%, 38%, and 40% were receiving non-heparin anticoagulation.
28388835	1	22	theme	non-heparin	189:199	arg1	anticoagulants					201:214	non-heparin anticoagulants	189:214	non-heparin anticoagulants	189:214	BACKGROUND Heparin-induced thrombocytopenia (HIT) is a thrombotic disorder usually prompting treatment with non-heparin anticoagulants.
28388835	3	23	theme	heparin	400:406	arg1	exposure					408:415	recent heparin exposure	393:415	recent heparin exposure	393:415	METHODS We analyzed data for 442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure.
28388835	10	24	from	[HR	1199:1201	arg1	likely					1176:1181	likely	1176:1181	likely	1176:1181	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	1	25	theme	Heparin-induced	92:106	arg1	thrombocytopenia					108:123	BACKGROUND Heparin-induced thrombocytopenia	81:123	BACKGROUND Heparin-induced thrombocytopenia (HIT)	81:129	BACKGROUND Heparin-induced thrombocytopenia (HIT) is a thrombotic disorder usually prompting treatment with non-heparin anticoagulants.
28388835	1	25	theme	Heparin-induced	92:106	arg1	disorder					147:154	a thrombotic disorder	134:154	a thrombotic disorder usually prompting treatment with non-heparin anticoagulants	134:214	BACKGROUND Heparin-induced thrombocytopenia (HIT) is a thrombotic disorder usually prompting treatment with non-heparin anticoagulants.
28388835	1	25	theme	Heparin-induced	92:106	arg1	HIT					126:128	HIT	126:128	HIT	126:128	BACKGROUND Heparin-induced thrombocytopenia (HIT) is a thrombotic disorder usually prompting treatment with non-heparin anticoagulants.
28388835	5	26	theme	Secondary	517:525	arg1	outcomes					527:534	Secondary outcomes	517:534	Secondary outcomes	517:534	Secondary outcomes included bleeding and the effect of anticoagulation.
28388835	0	27	theme	Clinical	0:7	arg1	outcomes					9:16	Clinical outcomes	0:16	Clinical outcomes in a cohort of patients with heparin-induced thrombocytopenia	0:78	Clinical outcomes in a cohort of patients with heparin-induced thrombocytopenia.
28388835	9	28	theme	non-heparin	1000:1010	arg1	anticoagulation					1012:1026	non-heparin anticoagulation	1000:1026	non-heparin anticoagulation	1000:1026	The composite endpoint occurred in 48%, 36%, and 17% (P = .01) of which 61%, 38%, and 40% were receiving non-heparin anticoagulation.
28388835	13	29	theme	endpoint	1461:1468	arg1	events					1470:1475	composite endpoint events	1451:1475	composite endpoint events in either HIT group	1451:1495	Non-heparin anticoagulation was not associated with a reduction in composite endpoint events in either HIT group.
28388835	6	30	contain	had	668:670	arg1	284					658:660	284	658:660	284	658:660	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	6	30	contain	had	668:670	arg2	HIT					672:674	HIT	672:674	HIT	672:674	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	6	30	contain	had	668:670	arg1	%					665:665	64%	663:665	64%	663:665	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	13	31	theme	Non-heparin	1384:1394	arg1	anticoagulation					1396:1410	Non-heparin anticoagulation	1384:1410	Non-heparin anticoagulation	1384:1410	Non-heparin anticoagulation was not associated with a reduction in composite endpoint events in either HIT group.
28388835	8	32	theme	Non-heparin	835:845	arg1	anticoagulation					847:861	Non-heparin anticoagulation	835:861	Non-heparin anticoagulation	835:861	Non-heparin anticoagulation was begun in 80%, 56%, and 45%.
28388835	13	33	from	reduction	1438:1446	arg1	events					1470:1475	composite endpoint events	1451:1475	composite endpoint events in either HIT group	1451:1495	Non-heparin anticoagulation was not associated with a reduction in composite endpoint events in either HIT group.
28388835	7	34	theme	high	757:760	arg1	score					767:771	An intermediate or high "4T" score	738:771	An intermediate or high "4T" score	738:771	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	14	35	theme	high	1531:1534	arg1	risks					1536:1540	high risks	1531:1540	high risks of death	1531:1549	INTERPRETATION HIT patients have high risks of death, limb amputation/gangrene, thrombosis, and bleeding.
28388835	3	36	theme	recent	393:398	arg1	exposure					408:415	recent heparin exposure	393:415	recent heparin exposure	393:415	METHODS We analyzed data for 442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure.
28388835	15	37	from	patients	1787:1794	arg1	risks					1740:1744	the relative risks	1727:1744	the relative risks of thrombosis and bleeding in individual patients	1727:1794	Non-heparin anticoagulant treatment may not benefit all patients and should be considered only after careful assessment of the relative risks of thrombosis and bleeding in individual patients.
28388835	15	38	theme	careful	1705:1711	arg1	assessment					1713:1722	careful assessment	1705:1722	careful assessment of the relative risks of thrombosis and bleeding in individual patients	1705:1794	Non-heparin anticoagulant treatment may not benefit all patients and should be considered only after careful assessment of the relative risks of thrombosis and bleeding in individual patients.
28388835	10	39	theme	no	1047:1048	arg1	group					1054:1058	the no HIT group	1043:1058	the no HIT group	1043:1058	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	0	40	theme	heparin-induced	47:61	arg1	thrombocytopenia					63:78	heparin-induced thrombocytopenia	47:78	heparin-induced thrombocytopenia	47:78	Clinical outcomes in a cohort of patients with heparin-induced thrombocytopenia.
28388835	10	41	theme	HIT	1195:1197	arg1	[HR					1199:1201	isolated HIT [HR	1186:1201	isolated HIT [HR	1186:1201	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	11	42	dep	increased	1250:1258	arg1	HR					1261:1262	HR 1.77	1261:1267	HR 1.77	1261:1267	Importantly, risk increased (HR 1.77, P = .02) after platelet transfusion.
28388835	11	42	dep	increased	1250:1258	arg1	P = .02					1270:1276	P = .02	1270:1276	P = .02	1270:1276	Importantly, risk increased (HR 1.77, P = .02) after platelet transfusion.
28388835	7	43	theme	intermediate	741:752	arg1	score					767:771	An intermediate or high "4T" score	738:771	An intermediate or high "4T" score	738:771	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	10	44	from	likely	1107:1112	arg1	1.35-4.55					1133:1141	1.35-4.55	1133:1141	1.35-4.55	1133:1141	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	10	44	from	likely	1107:1112	arg1	2.48					1127:1130	HIT-T [HR 2.48	1117:1130	HIT-T [HR 2.48 (1.35-4.55), P = .003)]	1117:1154	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	6	45	theme	Seventy-one	598:608	arg1	patients					610:617	FINDINGS Seventy-one patients	589:617	FINDINGS Seventy-one patients (16%)	589:623	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	6	45	theme	Seventy-one	598:608	arg1	%					622:622	16%	620:622	16%	620:622	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	10	46	theme	isolated	1186:1193	arg1	[HR					1199:1201	isolated HIT [HR	1186:1201	isolated HIT [HR	1186:1201	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	4	47	theme	limb	471:474	arg1	amputation/gangrene					476:494	limb amputation/gangrene	471:494	limb amputation/gangrene	471:494	The primary outcome was a composite endpoint (death, limb amputation/gangrene, or new thrombosis).
28388835	2	48	theme	treatments	248:257	arg1	benefits					221:228	benefits	221:228	benefits	221:228	The benefits and risks of such treatments have not been fully assessed.
28388835	2	48	theme	treatments	248:257	arg1	risks					234:238	risks	234:238	risks	234:238	The benefits and risks of such treatments have not been fully assessed.
28388835	15	49	theme	individual	1776:1785	arg1	patients					1787:1794	individual patients	1776:1794	individual patients	1776:1794	Non-heparin anticoagulant treatment may not benefit all patients and should be considered only after careful assessment of the relative risks of thrombosis and bleeding in individual patients.
28388835	6	50	theme	isolated	696:703	arg1	thrombosis					684:693	thrombosis	684:693	thrombosis (isolated HIT)	684:708	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	6	50	theme	isolated	696:703	arg1	HIT					705:707	isolated HIT	696:707	isolated HIT	696:707	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	6	51	theme	FINDINGS	589:596	arg1	patients					610:617	FINDINGS Seventy-one patients	589:617	FINDINGS Seventy-one patients (16%)	589:623	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	6	51	theme	FINDINGS	589:596	arg1	%					622:622	16%	620:622	16%	620:622	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	6	52	contain	had	625:627	arg1	patients					610:617	FINDINGS Seventy-one patients	589:617	FINDINGS Seventy-one patients (16%)	589:623	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	6	52	contain	had	625:627	arg1	%					622:622	16%	620:622	16%	620:622	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	6	52	contain	had	625:627	arg2	HIT					629:631	HIT	629:631	HIT	629:631	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	3	53	theme	heparin-platelet	349:364	arg1	test					384:387	a positive heparin-platelet factor 4 antibody test	338:387	a positive heparin-platelet factor 4 antibody test	338:387	METHODS We analyzed data for 442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure.
28388835	2	54	theme	such	243:246	arg1	treatments					248:257	such treatments	243:257	such treatments	243:257	The benefits and risks of such treatments have not been fully assessed.
28388835	10	55	theme	composite	1065:1073	arg1	1.66					1203:1206	1.66	1203:1206	1.66	1203:1206	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	10	55	theme	composite	1065:1073	arg1	endpoint					1075:1082	the composite endpoint	1061:1082	the composite endpoint	1061:1082	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	10	55	theme	composite	1065:1073	arg1	likely					1107:1112	likely	1107:1112	likely	1107:1112	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	10	56	dep	1.66	1203:1206	arg1	P = .071					1221:1228	P = .071	1221:1228	P = .071	1221:1228	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	0	57	theme	patients	33:40	arg1	cohort					23:28	a cohort	21:28	a cohort of patients with heparin-induced thrombocytopenia	21:78	Clinical outcomes in a cohort of patients with heparin-induced thrombocytopenia.
28388835	10	58	theme	[HR	1123:1125	arg1	1.35-4.55					1133:1141	1.35-4.55	1133:1141	1.35-4.55	1133:1141	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	10	58	theme	[HR	1123:1125	arg1	2.48					1127:1130	HIT-T [HR 2.48	1117:1130	HIT-T [HR 2.48 (1.35-4.55), P = .003)]	1117:1154	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	15	59	theme	thrombosis	1749:1758	arg1	risks					1740:1744	the relative risks	1727:1744	the relative risks of thrombosis and bleeding in individual patients	1727:1794	Non-heparin anticoagulant treatment may not benefit all patients and should be considered only after careful assessment of the relative risks of thrombosis and bleeding in individual patients.
28388835	13	60	from	events	1470:1475	arg1	group					1491:1495	either HIT group	1480:1495	either HIT group	1480:1495	Non-heparin anticoagulation was not associated with a reduction in composite endpoint events in either HIT group.
28388835	15	61	theme	bleeding	1764:1771	arg1	risks					1740:1744	the relative risks	1727:1744	the relative risks of thrombosis and bleeding in individual patients	1727:1794	Non-heparin anticoagulant treatment may not benefit all patients and should be considered only after careful assessment of the relative risks of thrombosis and bleeding in individual patients.
28388835	10	62	from	likely	1176:1181	arg1	[HR					1199:1201	isolated HIT [HR	1186:1201	isolated HIT [HR	1186:1201	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	15	63	theme	Non-heparin	1604:1614	arg1	treatment					1630:1638	Non-heparin anticoagulant treatment	1604:1638	Non-heparin anticoagulant treatment	1604:1638	Non-heparin anticoagulant treatment may not benefit all patients and should be considered only after careful assessment of the relative risks of thrombosis and bleeding in individual patients.
28388835	15	64	theme	relative	1731:1738	arg1	risks					1740:1744	the relative risks	1727:1744	the relative risks of thrombosis and bleeding in individual patients	1727:1794	Non-heparin anticoagulant treatment may not benefit all patients and should be considered only after careful assessment of the relative risks of thrombosis and bleeding in individual patients.
28388835	5	65	theme	anticoagulation	572:586	arg1	effect					562:567	the effect	558:567	the effect of anticoagulation	558:586	Secondary outcomes included bleeding and the effect of anticoagulation.
28388835	10	66	from	2.48	1127:1130	arg1	1.66					1203:1206	1.66	1203:1206	1.66	1203:1206	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	10	66	from	2.48	1127:1130	arg1	endpoint					1075:1082	the composite endpoint	1061:1082	the composite endpoint	1061:1082	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	10	66	from	2.48	1127:1130	arg1	likely					1107:1112	likely	1107:1112	likely	1107:1112	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	2	67	dep	benefits	221:228	arg1	The					217:219	The	217:219	The	217:219	The benefits and risks of such treatments have not been fully assessed.
28388835	15	68	theme	anticoagulant	1616:1628	arg1	treatment					1630:1638	Non-heparin anticoagulant treatment	1604:1638	Non-heparin anticoagulant treatment	1604:1638	Non-heparin anticoagulant treatment may not benefit all patients and should be considered only after careful assessment of the relative risks of thrombosis and bleeding in individual patients.
28388835	15	69	theme	risks	1740:1744	arg1	assessment					1713:1722	careful assessment	1705:1722	careful assessment of the relative risks of thrombosis and bleeding in individual patients	1705:1794	Non-heparin anticoagulant treatment may not benefit all patients and should be considered only after careful assessment of the relative risks of thrombosis and bleeding in individual patients.
28388835	11	70	theme	platelet	1285:1292	arg1	transfusion					1294:1304	platelet transfusion	1285:1304	platelet transfusion	1285:1304	Importantly, risk increased (HR 1.77, P = .02) after platelet transfusion.
28388835	1	71	theme	thrombotic	136:145	arg1	thrombocytopenia					108:123	BACKGROUND Heparin-induced thrombocytopenia	81:123	BACKGROUND Heparin-induced thrombocytopenia (HIT)	81:129	BACKGROUND Heparin-induced thrombocytopenia (HIT) is a thrombotic disorder usually prompting treatment with non-heparin anticoagulants.
28388835	1	71	theme	thrombotic	136:145	arg1	disorder					147:154	a thrombotic disorder	134:154	a thrombotic disorder usually prompting treatment with non-heparin anticoagulants	134:214	BACKGROUND Heparin-induced thrombocytopenia (HIT) is a thrombotic disorder usually prompting treatment with non-heparin anticoagulants.
28388835	10	72	dep	likely	1107:1112	arg1	0.96-2.85					1209:1217	0.96-2.85	1209:1217	0.96-2.85	1209:1217	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	10	72	dep	likely	1107:1112	arg1	1.66					1203:1206	1.66	1203:1206	1.66	1203:1206	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	10	72	dep	likely	1107:1112	arg1	endpoint					1075:1082	the composite endpoint	1061:1082	the composite endpoint	1061:1082	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	10	72	dep	likely	1107:1112	arg1	likely					1107:1112	likely	1107:1112	likely	1107:1112	Compared with the no HIT group, the composite endpoint was significantly more likely in HIT-T [HR 2.48 (1.35-4.55), P = .003)] and marginally more likely in isolated HIT [HR 1.66 (0.96-2.85), P = .071].
28388835	3	73	theme	positive	340:347	arg1	test					384:387	a positive heparin-platelet factor 4 antibody test	338:387	a positive heparin-platelet factor 4 antibody test	338:387	METHODS We analyzed data for 442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure.
28388835	4	74	theme	composite	444:452	arg1	endpoint					454:461	a composite endpoint	442:461	a composite endpoint (death, limb amputation/gangrene, or new thrombosis)	442:514	The primary outcome was a composite endpoint (death, limb amputation/gangrene, or new thrombosis).
28388835	4	74	theme	composite	444:452	arg1	outcome					430:436	The primary outcome	418:436	The primary outcome	418:436	The primary outcome was a composite endpoint (death, limb amputation/gangrene, or new thrombosis).
28388835	15	75	from	risks	1740:1744	arg1	patients					1787:1794	individual patients	1776:1794	individual patients	1776:1794	Non-heparin anticoagulant treatment may not benefit all patients and should be considered only after careful assessment of the relative risks of thrombosis and bleeding in individual patients.
28388835	0	76	with	patients	33:40	arg1	thrombocytopenia					63:78	heparin-induced thrombocytopenia	47:78	heparin-induced thrombocytopenia	47:78	Clinical outcomes in a cohort of patients with heparin-induced thrombocytopenia.
28388835	14	77	theme	death	1545:1549	arg1	risks					1536:1540	high risks	1531:1540	high risks of death	1531:1549	INTERPRETATION HIT patients have high risks of death, limb amputation/gangrene, thrombosis, and bleeding.
28388835	3	78	dep	METHODS	289:295	arg1	analyzed					300:307	analyzed	300:307	analyzed data for 442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure	300:415	METHODS We analyzed data for 442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure.
28388835	3	79	contain	having	331:336	arg2	test					384:387	a positive heparin-platelet factor 4 antibody test	338:387	a positive heparin-platelet factor 4 antibody test	338:387	METHODS We analyzed data for 442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure.
28388835	3	79	contain	having	331:336	arg2	exposure					408:415	recent heparin exposure	393:415	recent heparin exposure	393:415	METHODS We analyzed data for 442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure.
28388835	3	79	contain	having	331:336	arg1	patients					322:329	442 patients	318:329	442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure	318:415	METHODS We analyzed data for 442 patients having a positive heparin-platelet factor 4 antibody test and recent heparin exposure.
28388835	7	80	theme	groups	827:832	arg1	groups					827:832	the three respective groups	806:832	the three respective groups	806:832	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	7	80	theme	groups	827:832	arg1	%					801:801	8%	800:801	8%	800:801	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	7	80	theme	groups	827:832	arg1	%					788:788	85%	786:788	85%	786:788	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	7	80	theme	groups	827:832	arg1	%					793:793	58%	791:793	58%	791:793	An intermediate or high "4T" score was found in 85%, 58%, and 8% of the three respective groups.
28388835	4	81	dep	endpoint	454:461	arg1	death					464:468	death	464:468	death	464:468	The primary outcome was a composite endpoint (death, limb amputation/gangrene, or new thrombosis).
28388835	4	81	dep	endpoint	454:461	arg1	amputation/gangrene					476:494	limb amputation/gangrene	471:494	limb amputation/gangrene	471:494	The primary outcome was a composite endpoint (death, limb amputation/gangrene, or new thrombosis).
28388835	4	81	dep	endpoint	454:461	arg1	thrombosis					504:513	new thrombosis	500:513	new thrombosis	500:513	The primary outcome was a composite endpoint (death, limb amputation/gangrene, or new thrombosis).
28388835	6	82	contain	have	728:731	arg2	HIT					733:735	HIT	733:735	HIT	733:735	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	6	82	contain	have	728:731	arg1	87					711:712	87	711:712	87	711:712	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28388835	6	82	contain	have	728:731	arg1	%					717:717	20%	715:717	20%	715:717	FINDINGS Seventy-one patients (16%) had HIT with thrombosis (HIT-T); 284 (64%) had HIT without thrombosis (isolated HIT); 87 (20%) did not have HIT.
28578269	6	0	theme	procedure	1399:1407	arg1	effects					1357:1363	the effects	1353:1363	the effects of mixture composition and mixing procedure on the particle yield	1353:1429	Also investigated are the effects of mixture composition and mixing procedure on the particle yield.
28578269	5	1	theme	size	1271:1274	arg1	increase					1276:1283	the most uniform/gradual size increase	1246:1283	the most uniform/gradual size increase	1246:1283	Though the particle size increases sigmoidally with the NaCl concentration for all three mixing methods, the dilution method delivers the most uniform/gradual size increase - i.e., it provides the most precise control.
28578269	2	2	dep	ranges	454:459	arg1	size					489:492	the average particle size	468:492	the average particle size	468:492	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	2	2	dep	ranges	454:459	arg1	varied					502:507	varied	502:507	varied	502:507	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	7	3	theme	particle	1449:1456	arg1	yield					1458:1462	the particle yield	1445:1462	the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol	1445:1595	These reveal the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol; thus, at elevated NaCl concentrations, it may be beneficial to increase chitosan and TPP concentrations to ensure high particle yields.
28578269	7	4	theme	particle	1717:1724	arg1	yields					1726:1731	high particle yields	1712:1731	high particle yields	1712:1731	These reveal the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol; thus, at elevated NaCl concentrations, it may be beneficial to increase chitosan and TPP concentrations to ensure high particle yields.
28578269	9	5	theme	narrow	1977:1982	arg1	distributions					1989:2001	narrow size distributions	1977:2001	narrow size distributions	1977:2001	Taken together, these findings provide a simple and reliable method for extensively tuning chitosan/TPP particle size while maintaining narrow size distributions.
28578269	5	6	theme	precise	1314:1320	arg1	control					1322:1328	the most precise control	1305:1328	the most precise control	1305:1328	Though the particle size increases sigmoidally with the NaCl concentration for all three mixing methods, the dilution method delivers the most uniform/gradual size increase - i.e., it provides the most precise control.
28578269	4	7	dep	addition	923:930	arg1	2					911:911	2	911:911	2	911:911	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	7	8	theme	chitosan	1670:1677	arg1	concentrations					1687:1700	chitosan and TPP concentrations	1670:1700	chitosan and TPP concentrations	1670:1700	These reveal the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol; thus, at elevated NaCl concentrations, it may be beneficial to increase chitosan and TPP concentrations to ensure high particle yields.
28578269	7	9	theme	TPP	1683:1685	arg1	concentrations					1687:1700	chitosan and TPP concentrations	1670:1700	chitosan and TPP concentrations	1670:1700	These reveal the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol; thus, at elevated NaCl concentrations, it may be beneficial to increase chitosan and TPP concentrations to ensure high particle yields.
28578269	2	10	with	particles	326:334	arg1	distributions					353:365	narrow size distributions	341:365	narrow size distributions	341:365	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	9	11	theme	simple	1882:1887	arg1	method					1902:1907	a simple and reliable method	1880:1907	a simple and reliable method for extensively tuning chitosan/TPP particle size	1880:1957	Taken together, these findings provide a simple and reliable method for extensively tuning chitosan/TPP particle size while maintaining narrow size distributions.
28578269	2	12	theme	works	294:298	arg1	number					278:283	a number	276:283	a number of these works	276:298	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	4	13	theme	gelation-inhibiting	1013:1031	arg1	strength					1040:1047	a high (gelation-inhibiting) ionic strength	1005:1047	a high (gelation-inhibiting) ionic strength	1005:1047	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	4	14	theme	dilution	943:950	arg1	technique					952:960	a dilution technique	941:960	a dilution technique	941:960	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	3	15	with	particles	644:652	arg1	diameters					669:677	z-average diameters	659:677	z-average diameters ranging between roughly 100 and 900nm	659:715	Here we show how, by tuning the NaCl concentration in the parent chitosan and TPP solutions, low-polydispersity particles with z-average diameters ranging between roughly 100 and 900nm can be prepared.
28578269	4	16	theme	single-shot	890:900	arg1	mixing					902:907	(1) single-shot mixing	886:907	(1) single-shot mixing	886:907	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	9	17	theme	reliable	1893:1900	arg1	method					1902:1907	a simple and reliable method	1880:1907	a simple and reliable method for extensively tuning chitosan/TPP particle size	1880:1957	Taken together, these findings provide a simple and reliable method for extensively tuning chitosan/TPP particle size while maintaining narrow size distributions.
28578269	4	18	theme	ionic	1034:1038	arg1	strength					1040:1047	a high (gelation-inhibiting) ionic strength	1005:1047	a high (gelation-inhibiting) ionic strength	1005:1047	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	8	19	theme	solutions	1815:1823	arg1	pitfalls					1752:1759	possible pitfalls	1743:1759	possible pitfalls of the salt-assisted size control strategy (and their solutions)	1743:1824	Finally, possible pitfalls of the salt-assisted size control strategy (and their solutions) are discussed.
28578269	8	20	theme	salt-assisted	1768:1780	arg1	strategy					1795:1802	the salt-assisted size control strategy	1764:1802	the salt-assisted size control strategy	1764:1802	Finally, possible pitfalls of the salt-assisted size control strategy (and their solutions) are discussed.
28578269	8	21	theme	strategy	1795:1802	arg1	pitfalls					1752:1759	possible pitfalls	1743:1759	possible pitfalls of the salt-assisted size control strategy (and their solutions)	1743:1824	Finally, possible pitfalls of the salt-assisted size control strategy (and their solutions) are discussed.
28578269	8	22	theme	possible	1743:1750	arg1	pitfalls					1752:1759	possible pitfalls	1743:1759	possible pitfalls of the salt-assisted size control strategy (and their solutions)	1743:1824	Finally, possible pitfalls of the salt-assisted size control strategy (and their solutions) are discussed.
28578269	6	23	theme	particle	1416:1423	arg1	yield					1425:1429	the particle yield	1412:1429	the particle yield	1412:1429	Also investigated are the effects of mixture composition and mixing procedure on the particle yield.
28578269	7	24	theme	NaCl	1531:1534	arg1	concentration					1536:1548	the NaCl concentration	1527:1548	the NaCl concentration	1527:1548	These reveal the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol; thus, at elevated NaCl concentrations, it may be beneficial to increase chitosan and TPP concentrations to ensure high particle yields.
28578269	1	25	with	gelation	150:157	arg1	tripolyphosphate					176:191	tripolyphosphate	176:191	tripolyphosphate (TPP)	176:197	Nanoparticles prepared through the ionotropic gelation of chitosan with tripolyphosphate (TPP) have been extensively studied as vehicles for drug and gene delivery.
28578269	1	25	with	gelation	150:157	arg1	TPP					194:196	TPP	194:196	TPP	194:196	Nanoparticles prepared through the ionotropic gelation of chitosan with tripolyphosphate (TPP) have been extensively studied as vehicles for drug and gene delivery.
28578269	7	26	theme	NaCl	1616:1619	arg1	concentrations					1621:1634	elevated NaCl concentrations	1607:1634	elevated NaCl concentrations	1607:1634	These reveal the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol; thus, at elevated NaCl concentrations, it may be beneficial to increase chitosan and TPP concentrations to ensure high particle yields.
28578269	2	27	theme	preparing	316:324	arg1	particles					326:334	preparing particles	316:334	preparing particles with narrow size distributions	316:365	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	3	28	from	tuning	553:558	arg1	solutions					614:622	the parent chitosan and TPP solutions	586:622	the parent chitosan and TPP solutions	586:622	Here we show how, by tuning the NaCl concentration in the parent chitosan and TPP solutions, low-polydispersity particles with z-average diameters ranging between roughly 100 and 900nm can be prepared.
28578269	9	29	theme	chitosan/TPP	1932:1943	arg1	size					1954:1957	extensively tuning chitosan/TPP particle size	1913:1957	extensively tuning chitosan/TPP particle size	1913:1957	Taken together, these findings provide a simple and reliable method for extensively tuning chitosan/TPP particle size while maintaining narrow size distributions.
28578269	2	30	theme	size	449:452	arg1	ranges					454:459	narrow size ranges	442:459	narrow size ranges (where the average particle size was not varied by more than twofold)	442:529	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	4	31	dep	mixing	902:907	arg1	1					887:887	1	887:887	1	887:887	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	4	31	dep	mixing	902:907	arg1	addition					923:930	(2) dropwise addition	910:930	(2) dropwise addition	910:930	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	5	32	theme	mixing	1201:1206	arg1	methods					1208:1214	all three mixing methods	1191:1214	all three mixing methods	1191:1214	Though the particle size increases sigmoidally with the NaCl concentration for all three mixing methods, the dilution method delivers the most uniform/gradual size increase - i.e., it provides the most precise control.
28578269	2	33	theme	narrow	442:447	arg1	ranges					454:459	narrow size ranges	442:459	narrow size ranges (where the average particle size was not varied by more than twofold)	442:529	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	8	34	theme	size	1782:1785	arg1	strategy					1795:1802	the salt-assisted size control strategy	1764:1802	the salt-assisted size control strategy	1764:1802	Finally, possible pitfalls of the salt-assisted size control strategy (and their solutions) are discussed.
28578269	9	35	theme	tuning	1925:1930	arg1	size					1954:1957	extensively tuning chitosan/TPP particle size	1913:1957	extensively tuning chitosan/TPP particle size	1913:1957	Taken together, these findings provide a simple and reliable method for extensively tuning chitosan/TPP particle size while maintaining narrow size distributions.
28578269	7	36	from	concentrations	1621:1634	arg1	beneficial					1647:1656	beneficial	1647:1656	beneficial	1647:1656	These reveal the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol; thus, at elevated NaCl concentrations, it may be beneficial to increase chitosan and TPP concentrations to ensure high particle yields.
28578269	4	37	theme	high	1007:1010	arg1	strength					1040:1047	a high (gelation-inhibiting) ionic strength	1005:1047	a high (gelation-inhibiting) ionic strength	1005:1047	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	2	38	theme	particle	480:487	arg1	size					489:492	the average particle size	468:492	the average particle size	468:492	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	2	38	theme	particle	480:487	arg1	varied					502:507	varied	502:507	varied	502:507	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	5	39	theme	dilution	1221:1228	arg1	method					1230:1235	the dilution method	1217:1235	the dilution method	1217:1235	Though the particle size increases sigmoidally with the NaCl concentration for all three mixing methods, the dilution method delivers the most uniform/gradual size increase - i.e., it provides the most precise control.
28578269	5	40	theme	particle	1123:1130	arg1	size					1132:1135	the particle size	1119:1135	the particle size	1119:1135	Though the particle size increases sigmoidally with the NaCl concentration for all three mixing methods, the dilution method delivers the most uniform/gradual size increase - i.e., it provides the most precise control.
28578269	3	41	theme	NaCl	564:567	arg1	concentration					569:581	the NaCl concentration	560:581	the NaCl concentration	560:581	Here we show how, by tuning the NaCl concentration in the parent chitosan and TPP solutions, low-polydispersity particles with z-average diameters ranging between roughly 100 and 900nm can be prepared.
28578269	6	42	theme	mixing	1392:1397	arg1	procedure					1399:1407	mixing procedure	1392:1407	mixing procedure	1392:1407	Also investigated are the effects of mixture composition and mixing procedure on the particle yield.
28578269	4	43	dep	depends	786:792	arg1	comparing					875:883	comparing	875:883	comparing	875:883	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	2	44	theme	average	472:478	arg1	size					489:492	the average particle size	468:492	the average particle size	468:492	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	2	44	theme	average	472:478	arg1	varied					502:507	varied	502:507	varied	502:507	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	4	45	theme	dropwise	914:921	arg1	addition					923:930	(2) dropwise addition	910:930	(2) dropwise addition	910:930	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	9	46	theme	particle	1945:1952	arg1	size					1954:1957	extensively tuning chitosan/TPP particle size	1913:1957	extensively tuning chitosan/TPP particle size	1913:1957	Taken together, these findings provide a simple and reliable method for extensively tuning chitosan/TPP particle size while maintaining narrow size distributions.
28578269	4	47	theme	particles	776:784	arg1	size					762:765	the size	758:765	the size of these particles	758:784	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	4	48	theme	lower	1069:1073	arg1	strengths					1081:1089	lower ionic strengths	1069:1089	lower ionic strengths	1069:1089	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	4	49	theme	chitosan	843:850	arg1	solution					852:859	the chitosan solution	839:859	the chitosan solution	839:859	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	3	50	theme	TPP	610:612	arg1	solutions					614:622	the parent chitosan and TPP solutions	586:622	the parent chitosan and TPP solutions	586:622	Here we show how, by tuning the NaCl concentration in the parent chitosan and TPP solutions, low-polydispersity particles with z-average diameters ranging between roughly 100 and 900nm can be prepared.
28578269	1	51	theme	ionotropic	139:148	arg1	gelation					150:157	the ionotropic gelation	135:157	the ionotropic gelation of chitosan with tripolyphosphate (TPP)	135:197	Nanoparticles prepared through the ionotropic gelation of chitosan with tripolyphosphate (TPP) have been extensively studied as vehicles for drug and gene delivery.
28578269	0	52	theme	tunable	67:73	arg1	size					75:78	highly tunable size	60:78	highly tunable size	60:78	Preparation of chitosan/tripolyphosphate nanoparticles with highly tunable size and low polydispersity.
28578269	7	53	theme	chitosan/TPP	1485:1496	arg1	concentration					1498:1510	the chitosan/TPP concentration	1481:1510	the chitosan/TPP concentration	1481:1510	These reveal the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol; thus, at elevated NaCl concentrations, it may be beneficial to increase chitosan and TPP concentrations to ensure high particle yields.
28578269	8	54	theme	control	1787:1793	arg1	strategy					1795:1802	the salt-assisted size control strategy	1764:1802	the salt-assisted size control strategy	1764:1802	Finally, possible pitfalls of the salt-assisted size control strategy (and their solutions) are discussed.
28578269	6	55	dep	effects	1357:1363	arg1	investigated					1336:1347	investigated	1336:1347	investigated	1336:1347	Also investigated are the effects of mixture composition and mixing procedure on the particle yield.
28578269	2	56	theme	size	348:351	arg1	distributions					353:365	narrow size distributions	341:365	narrow size distributions	341:365	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	5	57	theme	NaCl	1168:1171	arg1	concentration					1173:1185	the NaCl concentration	1164:1185	the NaCl concentration for all three mixing methods	1164:1214	Though the particle size increases sigmoidally with the NaCl concentration for all three mixing methods, the dilution method delivers the most uniform/gradual size increase - i.e., it provides the most precise control.
28578269	7	58	theme	mixing	1581:1586	arg1	protocol					1588:1595	the mixing protocol	1577:1595	the mixing protocol	1577:1595	These reveal the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol; thus, at elevated NaCl concentrations, it may be beneficial to increase chitosan and TPP concentrations to ensure high particle yields.
28578269	3	59	theme	z-average	659:667	arg1	diameters					669:677	z-average diameters	659:677	z-average diameters ranging between roughly 100 and 900nm	659:715	Here we show how, by tuning the NaCl concentration in the parent chitosan and TPP solutions, low-polydispersity particles with z-average diameters ranging between roughly 100 and 900nm can be prepared.
28578269	3	60	theme	parent	590:595	arg1	chitosan					597:604	parent chitosan	590:604	parent chitosan	590:604	Here we show how, by tuning the NaCl concentration in the parent chitosan and TPP solutions, low-polydispersity particles with z-average diameters ranging between roughly 100 and 900nm can be prepared.
28578269	4	61	theme	ionic	1075:1079	arg1	strengths					1081:1089	lower ionic strengths	1069:1089	lower ionic strengths	1069:1089	Further, we explore how the size of these particles depends on the method by which the TPP is mixed into the chitosan solution, specifically comparing: (1) single-shot mixing; (2) dropwise addition; and (3) a dilution technique, where chitosan and TPP are codissolved at a high (gelation-inhibiting) ionic strength and then diluted to lower ionic strengths to trigger gelation.
28578269	2	62	theme	narrow	341:346	arg1	distributions					353:365	narrow size distributions	341:365	narrow size distributions	341:365	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
28578269	6	63	theme	composition	1376:1386	arg1	effects					1357:1363	the effects	1353:1363	the effects of mixture composition and mixing procedure on the particle yield	1353:1429	Also investigated are the effects of mixture composition and mixing procedure on the particle yield.
28578269	1	64	theme	drug	245:248	arg1	delivery					259:266	drug and gene delivery	245:266	delivery	259:266	Nanoparticles prepared through the ionotropic gelation of chitosan with tripolyphosphate (TPP) have been extensively studied as vehicles for drug and gene delivery.
28578269	7	65	theme	high	1712:1715	arg1	yields					1726:1731	high particle yields	1712:1731	high particle yields	1712:1731	These reveal the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol; thus, at elevated NaCl concentrations, it may be beneficial to increase chitosan and TPP concentrations to ensure high particle yields.
28578269	3	66	theme	low-polydispersity	625:642	arg1	particles					644:652	low-polydispersity particles	625:652	low-polydispersity particles with z-average diameters ranging between roughly 100 and 900nm	625:715	Here we show how, by tuning the NaCl concentration in the parent chitosan and TPP solutions, low-polydispersity particles with z-average diameters ranging between roughly 100 and 900nm can be prepared.
28578269	3	67	theme	chitosan	597:604	arg1	solutions					614:622	the parent chitosan and TPP solutions	586:622	the parent chitosan and TPP solutions	586:622	Here we show how, by tuning the NaCl concentration in the parent chitosan and TPP solutions, low-polydispersity particles with z-average diameters ranging between roughly 100 and 900nm can be prepared.
28578269	1	68	theme	chitosan	162:169	arg1	gelation					150:157	the ionotropic gelation	135:157	the ionotropic gelation of chitosan with tripolyphosphate (TPP)	135:197	Nanoparticles prepared through the ionotropic gelation of chitosan with tripolyphosphate (TPP) have been extensively studied as vehicles for drug and gene delivery.
28578269	6	69	theme	mixture	1368:1374	arg1	composition					1376:1386	mixture composition	1368:1386	mixture composition	1368:1386	Also investigated are the effects of mixture composition and mixing procedure on the particle yield.
28578269	0	70	theme	low	84:86	arg1	polydispersity					88:101	low polydispersity	84:101	low polydispersity	84:101	Preparation of chitosan/tripolyphosphate nanoparticles with highly tunable size and low polydispersity.
28578269	9	71	theme	size	1984:1987	arg1	distributions					1989:2001	narrow size distributions	1977:2001	narrow size distributions	1977:2001	Taken together, these findings provide a simple and reliable method for extensively tuning chitosan/TPP particle size while maintaining narrow size distributions.
28578269	6	72	from	effects	1357:1363	arg1	yield					1425:1429	the particle yield	1412:1429	the particle yield	1412:1429	Also investigated are the effects of mixture composition and mixing procedure on the particle yield.
28578269	7	73	theme	elevated	1607:1614	arg1	concentrations					1621:1634	elevated NaCl concentrations	1607:1634	elevated NaCl concentrations	1607:1634	These reveal the particle yield to increase with the chitosan/TPP concentration, decrease with the NaCl concentration, and vary only weakly with the mixing protocol; thus, at elevated NaCl concentrations, it may be beneficial to increase chitosan and TPP concentrations to ensure high particle yields.
28578269	1	74	theme	gene	254:257	arg1	delivery					259:266	drug and gene delivery	245:266	delivery	259:266	Nanoparticles prepared through the ionotropic gelation of chitosan with tripolyphosphate (TPP) have been extensively studied as vehicles for drug and gene delivery.
28578269	0	75	theme	chitosan/tripolyphosphate	15:39	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan/tripolyphosphate	0:39	Preparation of chitosan/tripolyphosphate nanoparticles with highly tunable size and low polydispersity.
28578269	5	76	theme	uniform/gradual	1255:1269	arg1	increase					1276:1283	the most uniform/gradual size increase	1246:1283	the most uniform/gradual size increase	1246:1283	Though the particle size increases sigmoidally with the NaCl concentration for all three mixing methods, the dilution method delivers the most uniform/gradual size increase - i.e., it provides the most precise control.
28578269	2	77	theme	monodisperse	372:383	arg1	particles					385:393	the monodisperse particles	368:393	the monodisperse particles produced by these methods	368:419	Though a number of these works have focused on preparing particles with narrow size distributions, the monodisperse particles produced by these methods have been limited to narrow size ranges (where the average particle size was not varied by more than twofold).
25633223	6	0	theme	biocomposite	1274:1285	arg1	material					1287:1294	1 wt % EPE/BC biocomposite material	1260:1294	1 wt % EPE/BC biocomposite material with the best properties	1260:1319	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	1	1	from	biosynthesis	325:336	arg1	culture					373:379	a static culture	364:379	a static culture from Gluconacetobacter xylinum	364:410	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	1	1	from	biosynthesis	325:336	arg1	xylinum					404:410	Gluconacetobacter xylinum	386:410	Gluconacetobacter xylinum	386:410	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	4	2	theme	EPE	870:872	arg1	copolymer					880:888	EPE block copolymer	870:888	EPE block copolymer	870:888	Structural properties of EPE/BC biocomposites showed a strong effect of EPE block copolymer on the morphology of the BC mats.
25633223	6	3	theme	EPE/BC	1166:1171	arg1	biocomposites					1173:1185	EPE/BC biocomposites	1166:1185	EPE/BC biocomposites	1166:1185	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	0	4	theme	Nano-	0:4	arg1	properties					47:56	Nano- and macroscale structural and mechanical properties	0:56	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	0:129	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites.
25633223	7	5	theme	based	1456:1460	arg1	biocomposites					1462:1474	BC mat based biocomposites	1449:1474	BC mat based biocomposites with tunable properties	1449:1498	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	4	6	theme	copolymer	880:888	arg1	effect					860:865	a strong effect	851:865	a strong effect of EPE block copolymer on the morphology of the BC mats	851:921	Structural properties of EPE/BC biocomposites showed a strong effect of EPE block copolymer on the morphology of the BC mats.
25633223	7	7	theme	copolymers	1416:1425	arg1	utilization					1381:1391	the utilization	1377:1391	the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties	1377:1498	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	6	8	theme	mat	1250:1252	arg1	stability					1204:1212	thermal stability	1196:1212	thermal stability	1196:1212	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	6	8	theme	mat	1250:1252	arg1	properties					1229:1238	mechanical properties	1218:1238	mechanical properties	1218:1238	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	2	9	theme	novel	568:572	arg1	biocomposites					574:586	the novel biocomposites	564:586	the novel biocomposites	564:586	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	7	10	theme	water-soluble	1396:1408	arg1	copolymers					1416:1425	water-soluble block copolymers	1396:1425	water-soluble block copolymers	1396:1425	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	3	11	theme	PEO	764:766	arg1	block					768:772	the PEO block	760:772	the PEO block of EPE block copolymer	760:795	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	6	12	theme	BC	1247:1248	arg1	mat					1250:1252	the BC mat	1243:1252	the BC mat	1243:1252	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	4	13	from	effect	860:865	arg1	morphology					897:906	the morphology	893:906	the morphology of the BC mats	893:921	Structural properties of EPE/BC biocomposites showed a strong effect of EPE block copolymer on the morphology of the BC mats.
25633223	1	14	theme	oxide-b-propylene	230:246	arg1	oxide					265:269	ethylene oxide-b-propylene oxide-b-ethylene oxide	221:269	ethylene oxide-b-propylene oxide-b-ethylene oxide	221:269	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	1	14	theme	oxide-b-propylene	230:246	arg1	poly					216:219	poly	216:219	poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE)	216:292	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	4	15	theme	BC	915:916	arg1	mats					918:921	the BC mats	911:921	the BC mats	911:921	Structural properties of EPE/BC biocomposites showed a strong effect of EPE block copolymer on the morphology of the BC mats.
25633223	3	16	theme	block	781:785	arg1	copolymer					787:795	EPE block copolymer	777:795	EPE block copolymer	777:795	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	6	17	theme	best	1305:1308	arg1	properties					1310:1319	the best properties	1301:1319	the best properties	1301:1319	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	6	18	theme	mechanical	1218:1227	arg1	properties					1229:1238	mechanical properties	1218:1238	mechanical properties	1218:1238	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	7	19	theme	new	1361:1363	arg1	strategy					1365:1372	new strategy	1361:1372	new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties	1361:1498	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	7	20	theme	composites	1345:1354	arg1	Biosynthesis					1322:1333	Biosynthesis	1322:1333	Biosynthesis of EPE/BC composites	1322:1354	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	0	21	theme	in	61:62	arg1	biocomposites					117:129	in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	61:129	in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	61:129	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites.
25633223	5	22	theme	final	1107:1111	arg1	biocomposites					1120:1132	the final EPE/BC biocomposites	1103:1132	the final EPE/BC biocomposites investigated by XRD	1103:1152	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	1	23	theme	cellulose	183:191	arg1	mat					198:200	bacterial cellulose (BC) mat	173:200	bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE)	173:292	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	2	24	theme	biocomposites	574:586	arg1	morphology					513:522	morphology	513:522	morphology	513:522	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	2	24	theme	biocomposites	574:586	arg1	structure					502:510	structure	502:510	structure	502:510	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	2	24	theme	biocomposites	574:586	arg1	properties					550:559	final properties	544:559	final properties of the novel biocomposites	544:586	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	2	24	theme	biocomposites	574:586	arg1	crystallinity					525:537	crystallinity	525:537	crystallinity	525:537	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	0	25	theme	synthesized	69:79	arg1	biocomposites					117:129	in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	61:129	in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	61:129	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites.
25633223	5	26	theme	biocomposites	1120:1132	arg1	crystallinity					1086:1098	crystallinity	1086:1098	crystallinity of the final EPE/BC biocomposites investigated by XRD	1086:1152	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	1	27	theme	BC	194:195	arg1	mat					198:200	bacterial cellulose (BC) mat	173:200	bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE)	173:292	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	2	28	theme	final	544:548	arg1	properties					550:559	final properties	544:559	final properties of the novel biocomposites	544:586	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	0	29	dep	in	61:62	arg1	situ					64:67	situ	64:67	situ	64:67	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites.
25633223	0	30	theme	cellulose/PEO-b-PPO-b-PEO	91:115	arg1	biocomposites					117:129	in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	61:129	in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	61:129	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites.
25633223	5	31	theme	content	970:976	arg1	increase					934:941	the increase	930:941	the increase of the EPE block copolymer content	930:976	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	4	32	theme	Structural	798:807	arg1	properties					809:818	Structural properties	798:818	Structural properties of EPE/BC biocomposites	798:842	Structural properties of EPE/BC biocomposites showed a strong effect of EPE block copolymer on the morphology of the BC mats.
25633223	1	33	theme	Gluconacetobacter	386:402	arg1	xylinum					404:410	Gluconacetobacter xylinum	386:410	Gluconacetobacter xylinum	386:410	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	7	34	theme	EPE/BC	1338:1343	arg1	composites					1345:1354	EPE/BC composites	1338:1354	EPE/BC composites	1338:1354	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	2	35	theme	water-soluble	465:477	arg1	copolymer					489:497	water-soluble EPE block copolymer	465:497	water-soluble EPE block copolymer	465:497	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	1	36	from	xylinum	404:410	arg1	biosynthesis					325:336	biosynthesis	325:336	biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum	325:410	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	1	36	from	xylinum	404:410	arg1	culture					373:379	a static culture	364:379	a static culture from Gluconacetobacter xylinum	364:410	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	0	37	theme	macroscale	10:19	arg1	properties					47:56	Nano- and macroscale structural and mechanical properties	0:56	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	0:129	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites.
25633223	5	38	theme	block	954:958	arg1	content					970:976	the EPE block copolymer content	946:976	the EPE block copolymer content	946:976	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	7	39	from	utilization	1381:1391	arg1	preparation					1434:1444	the preparation	1430:1444	the preparation of BC mat based biocomposites with tunable properties	1430:1498	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	2	40	theme	addition	431:438	arg1	effect					417:422	The effect	413:422	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites	413:586	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	5	41	theme	PEO	1019:1021	arg1	block					1023:1027	PEO block	1019:1027	PEO block	1019:1027	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	2	42	theme	block	483:487	arg1	copolymer					489:497	water-soluble EPE block copolymer	465:497	water-soluble EPE block copolymer	465:497	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	1	43	theme	cellulose	351:359	arg1	biosynthesis					325:336	biosynthesis	325:336	biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum	325:410	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	1	44	theme	oxide-b-ethylene	248:263	arg1	oxide					265:269	ethylene oxide-b-propylene oxide-b-ethylene oxide	221:269	ethylene oxide-b-propylene oxide-b-ethylene oxide	221:269	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	1	44	theme	oxide-b-ethylene	248:263	arg1	poly					216:219	poly	216:219	poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE)	216:292	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	3	45	theme	hydrogen	705:712	arg1	formation					719:727	hydrogen bond formation	705:727	hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer	705:795	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	4	46	theme	biocomposites	830:842	arg1	properties					809:818	Structural properties	798:818	Structural properties of EPE/BC biocomposites	798:842	Structural properties of EPE/BC biocomposites showed a strong effect of EPE block copolymer on the morphology of the BC mats.
25633223	5	47	theme	spherulites	1004:1014	arg1	generation					990:999	the generation	986:999	the generation of spherulites of PEO block	986:1027	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	7	48	theme	tunable	1481:1487	arg1	properties					1489:1498	tunable properties	1481:1498	tunable properties	1481:1498	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	6	49	theme	EPE/BC	1267:1272	arg1	material					1287:1294	1 wt % EPE/BC biocomposite material	1260:1294	1 wt % EPE/BC biocomposite material with the best properties	1260:1319	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	7	50	theme	biocomposites	1462:1474	arg1	preparation					1434:1444	the preparation	1430:1444	the preparation of BC mat based biocomposites with tunable properties	1430:1498	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	6	51	theme	%	1265:1265	arg1	material					1287:1294	1 wt % EPE/BC biocomposite material	1260:1294	1 wt % EPE/BC biocomposite material with the best properties	1260:1319	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	7	52	theme	BC	1449:1450	arg1	biocomposites					1462:1474	BC mat based biocomposites	1449:1474	BC mat based biocomposites with tunable properties	1449:1498	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	6	53	theme	wt	1262:1263	arg1	material					1287:1294	1 wt % EPE/BC biocomposite material	1260:1294	1 wt % EPE/BC biocomposite material with the best properties	1260:1319	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	7	54	theme	mat	1452:1454	arg1	biocomposites					1462:1474	BC mat based biocomposites	1449:1474	BC mat based biocomposites with tunable properties	1449:1498	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	3	55	theme	BC	753:754	arg1	BC					753:754	BC	753:754	BC	753:754	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	3	55	theme	BC	753:754	arg1	group					744:748	the OH group	737:748	the OH group of BC	737:754	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	3	55	theme	BC	753:754	arg1	block					768:772	the PEO block	760:772	the PEO block of EPE block copolymer	760:795	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	3	55	theme	BC	753:754	arg1	copolymer					787:795	EPE block copolymer	777:795	EPE block copolymer	777:795	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	4	56	theme	block	874:878	arg1	copolymer					880:888	EPE block copolymer	870:888	EPE block copolymer	870:888	Structural properties of EPE/BC biocomposites showed a strong effect of EPE block copolymer on the morphology of the BC mats.
25633223	3	57	theme	EPE	777:779	arg1	copolymer					787:795	EPE block copolymer	777:795	EPE block copolymer	777:795	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	7	58	theme	block	1410:1414	arg1	copolymers					1416:1425	water-soluble block copolymers	1396:1425	water-soluble block copolymers	1396:1425	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	5	59	theme	MO	1063:1064	arg1	technique					1066:1074	AFM and MO technique	1055:1074	AFM and MO technique	1055:1074	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	1	60	theme	ethylene	221:228	arg1	oxide					265:269	ethylene oxide-b-propylene oxide-b-ethylene oxide	221:269	ethylene oxide-b-propylene oxide-b-ethylene oxide	221:269	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	1	60	theme	ethylene	221:228	arg1	poly					216:219	poly	216:219	poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE)	216:292	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	1	61	theme	transparent	139:149	arg1	biocomposite					151:162	Highly transparent biocomposite	132:162	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE)	132:292	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	3	62	theme	copolymer	787:795	arg1	BC					753:754	BC	753:754	BC	753:754	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	3	62	theme	copolymer	787:795	arg1	group					744:748	the OH group	737:748	the OH group of BC	737:754	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	3	62	theme	copolymer	787:795	arg1	block					768:772	the PEO block	760:772	the PEO block of EPE block copolymer	760:795	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	3	62	theme	copolymer	787:795	arg1	copolymer					787:795	EPE block copolymer	777:795	EPE block copolymer	777:795	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	4	63	theme	mats	918:921	arg1	morphology					897:906	the morphology	893:906	the morphology of the BC mats	893:921	Structural properties of EPE/BC biocomposites showed a strong effect of EPE block copolymer on the morphology of the BC mats.
25633223	5	64	theme	AFM	1055:1057	arg1	technique					1066:1074	AFM and MO technique	1055:1074	AFM and MO technique	1055:1074	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	3	65	theme	High	630:633	arg1	compatibility					635:647	High compatibility	630:647	High compatibility between components	630:666	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	1	66	theme	bacterial	173:181	arg1	mat					198:200	bacterial cellulose (BC) mat	173:200	bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE)	173:292	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	6	67	theme	thermal	1196:1202	arg1	stability					1204:1212	thermal stability	1196:1212	thermal stability	1196:1212	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	0	68	theme	bacterial	81:89	arg1	biocomposites					117:129	in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	61:129	in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	61:129	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites.
25633223	5	69	theme	EPE/BC	1113:1118	arg1	biocomposites					1120:1132	the final EPE/BC biocomposites	1103:1132	the final EPE/BC biocomposites investigated by XRD	1103:1152	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	0	70	theme	biocomposites	117:129	arg1	properties					47:56	Nano- and macroscale structural and mechanical properties	0:56	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	0:129	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites.
25633223	1	71	theme	static	366:371	arg1	culture					373:379	a static culture	364:379	a static culture from Gluconacetobacter xylinum	364:410	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	5	72	theme	copolymer	960:968	arg1	content					970:976	the EPE block copolymer content	946:976	the EPE block copolymer content	946:976	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	7	73	with	biocomposites	1462:1474	arg1	properties					1489:1498	tunable properties	1481:1498	tunable properties	1481:1498	Biosynthesis of EPE/BC composites open new strategy to the utilization of water-soluble block copolymers in the preparation of BC mat based biocomposites with tunable properties.
25633223	0	74	theme	structural	21:30	arg1	properties					47:56	Nano- and macroscale structural and mechanical properties	0:56	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	0:129	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites.
25633223	1	75	dep	poly	216:219	arg1	copolymer					278:286	block copolymer	272:286	poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE)	216:292	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	5	76	theme	EPE	950:952	arg1	content					970:976	the EPE block copolymer content	946:976	the EPE block copolymer content	946:976	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	2	77	theme	culture	447:453	arg1	medium					455:460	the culture medium	443:460	the culture medium	443:460	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	0	78	theme	mechanical	36:45	arg1	properties					47:56	Nano- and macroscale structural and mechanical properties	0:56	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites	0:129	Nano- and macroscale structural and mechanical properties of in situ synthesized bacterial cellulose/PEO-b-PPO-b-PEO biocomposites.
25633223	2	79	theme	copolymer	489:497	arg1	addition					431:438	the addition	427:438	the addition to the culture medium of water-soluble EPE block copolymer	427:497	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	5	80	theme	block	1023:1027	arg1	spherulites					1004:1014	spherulites	1004:1014	spherulites of PEO block	1004:1027	Thus, the increase of the EPE block copolymer content lead to the generation of spherulites of PEO block, clearly visualized using AFM and MO technique, changing crystallinity of the final EPE/BC biocomposites investigated by XRD.
25633223	2	81	theme	EPE	479:481	arg1	copolymer					489:497	water-soluble EPE block copolymer	465:497	water-soluble EPE block copolymer	465:497	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	3	82	theme	bond	714:717	arg1	formation					719:727	hydrogen bond formation	705:727	hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer	705:795	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	4	83	theme	strong	853:858	arg1	effect					860:865	a strong effect	851:865	a strong effect of EPE block copolymer on the morphology of the BC mats	851:921	Structural properties of EPE/BC biocomposites showed a strong effect of EPE block copolymer on the morphology of the BC mats.
25633223	1	84	theme	bacterial	341:349	arg1	cellulose					351:359	bacterial cellulose	341:359	bacterial cellulose	341:359	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
25633223	2	85	from	effect	417:422	arg1	morphology					513:522	morphology	513:522	morphology	513:522	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	2	85	from	effect	417:422	arg1	structure					502:510	structure	502:510	structure	502:510	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	2	85	from	effect	417:422	arg1	properties					550:559	final properties	544:559	final properties of the novel biocomposites	544:586	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	2	85	from	effect	417:422	arg1	crystallinity					525:537	crystallinity	525:537	crystallinity	525:537	The effect of the addition to the culture medium of water-soluble EPE block copolymer on structure, morphology, crystallinity, and final properties of the novel biocomposites was investigated at nano- and macroscale.
25633223	6	86	with	material	1287:1294	arg1	properties					1310:1319	the best properties	1301:1319	the best properties	1301:1319	Generally, EPE/BC biocomposites maintain thermal stability and mechanical properties of the BC mat being 1 wt % EPE/BC biocomposite material with the best properties.
25633223	3	87	theme	OH	741:742	arg1	BC					753:754	BC	753:754	BC	753:754	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	3	87	theme	OH	741:742	arg1	group					744:748	the OH group	737:748	the OH group of BC	737:754	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	3	87	theme	OH	741:742	arg1	copolymer					787:795	EPE block copolymer	777:795	EPE block copolymer	777:795	High compatibility between components was confirmed by ATR-FTIR indicating hydrogen bond formation between the OH group of BC and the PEO block of EPE block copolymer.
25633223	4	88	theme	EPE/BC	823:828	arg1	biocomposites					830:842	EPE/BC biocomposites	823:842	EPE/BC biocomposites	823:842	Structural properties of EPE/BC biocomposites showed a strong effect of EPE block copolymer on the morphology of the BC mats.
25633223	1	89	theme	block	272:276	arg1	copolymer					278:286	block copolymer	272:286	poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE)	216:292	Highly transparent biocomposite based on bacterial cellulose (BC) mat modified with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block copolymer (EPE) were fabricated in situ during biosynthesis of bacterial cellulose in a static culture from Gluconacetobacter xylinum.
26076636	0	0	theme	agar/silver	87:97	arg1	nanoparticles					99:111	antimicrobial agar/silver nanoparticles	73:111	antimicrobial agar/silver nanoparticles	73:111	Amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films.
26076636	6	1	from	break	900:904	arg1	elongation					886:895	elongation	886:895	elongation at break	886:904	The water contact angle, tensile strength, and modulus decreased slightly, but elongation at break increased after AgNPs incorporation.
26076636	4	2	theme	Apparent	560:567	arg1	color					577:581	Apparent surface color	560:581	Apparent surface color	560:581	Apparent surface color and transmittance of agar films were greatly influenced by the AgNPs.
26076636	0	3	theme	antimicrobial	73:85	arg1	nanoparticles					99:111	antimicrobial agar/silver nanoparticles	73:111	antimicrobial agar/silver nanoparticles	73:111	Amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films.
26076636	6	4	theme	tensile	832:838	arg1	strength					840:847	tensile strength	832:847	tensile strength	832:847	The water contact angle, tensile strength, and modulus decreased slightly, but elongation at break increased after AgNPs incorporation.
26076636	0	5	theme	composite	113:121	arg1	films					123:127	composite films	113:127	composite films	113:127	Amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films.
26076636	7	6	theme	agar/AgNPs	947:956	arg1	films					972:976	The agar/AgNPs nanocomposite films	943:976	The agar/AgNPs nanocomposite films	943:976	The agar/AgNPs nanocomposite films possessed strong antibacterial activity against Listeria monocytogenes and Escherichia coli.
26076636	0	7	theme	nanoparticles	99:111	arg1	preparation					58:68	preparation	58:68	preparation of antimicrobial agar/silver nanoparticles	58:111	Amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films.
26076636	0	7	theme	nanoparticles	99:111	arg1	synthesis					20:28	synthesis	20:28	synthesis of silver nanoparticles	20:52	Amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films.
26076636	3	8	theme	dominant	538:545	arg1	111					548:550	111	548:550	111	548:550	XRD data demonstrated the crystalline structure of AgNPs with dominant (111) facet.
26076636	3	8	theme	dominant	538:545	arg1	facet					553:557	dominant (111) facet	538:557	dominant (111) facet	538:557	XRD data demonstrated the crystalline structure of AgNPs with dominant (111) facet.
26076636	4	9	theme	surface	569:575	arg1	color					577:581	Apparent surface color	560:581	Apparent surface color	560:581	Apparent surface color and transmittance of agar films were greatly influenced by the AgNPs.
26076636	1	10	theme	Silver	130:135	arg1	AgNPs					152:156	AgNPs	152:156	AgNPs	152:156	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	1	10	theme	Silver	130:135	arg1	nanoparticles					137:149	Silver nanoparticles	130:149	Silver nanoparticles (AgNPs)	130:157	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	4	11	theme	films	609:613	arg1	transmittance					587:599	transmittance	587:599	transmittance of agar films	587:613	Apparent surface color and transmittance of agar films were greatly influenced by the AgNPs.
26076636	4	11	theme	films	609:613	arg1	color					577:581	Apparent surface color	560:581	Apparent surface color	560:581	Apparent surface color and transmittance of agar films were greatly influenced by the AgNPs.
26076636	1	12	theme	amino	182:186	arg1	tryptophan					208:217	tryptophan	208:217	tryptophan	208:217	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	1	12	theme	amino	182:186	arg1	agents					244:249	reducing and capping agents	223:249	reducing and capping agents	223:249	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	1	12	theme	amino	182:186	arg1	acids					188:192	amino acids	182:192	amino acids (tyrosine and tryptophan)	182:218	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	1	12	theme	amino	182:186	arg1	tyrosine					195:202	tyrosine	195:202	tyrosine	195:202	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	3	13	theme	AgNPs	527:531	arg1	structure					514:522	the crystalline structure	498:522	the crystalline structure of AgNPs	498:531	XRD data demonstrated the crystalline structure of AgNPs with dominant (111) facet.
26076636	5	14	theme	thermal	740:746	arg1	stability					748:756	thermal stability	740:756	thermal stability	740:756	The incorporation of AgNPs into agar did not exhibit any change in chemical structure, thermal stability, moisture content, and water vapor permeability.
26076636	0	15	theme	Amino	0:4	arg1	acid					6:9	Amino acid	0:9	Amino acid	0:9	Amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films.
26076636	5	16	theme	water	781:785	arg1	permeability					793:804	water vapor permeability	781:804	water vapor permeability	781:804	The incorporation of AgNPs into agar did not exhibit any change in chemical structure, thermal stability, moisture content, and water vapor permeability.
26076636	2	17	theme	red	420:422	arg1	shift					424:428	a red shift	418:428	a red shift to ∼434 nm	418:439	The AgNPs solutions exhibited characteristic absorption peak at 420 nm that showed a red shift to ∼434 nm after forming composite with agar.
26076636	5	18	theme	vapor	787:791	arg1	permeability					793:804	water vapor permeability	781:804	water vapor permeability	781:804	The incorporation of AgNPs into agar did not exhibit any change in chemical structure, thermal stability, moisture content, and water vapor permeability.
26076636	6	19	theme	AgNPs	922:926	arg1	incorporation					928:940	AgNPs incorporation	922:940	AgNPs incorporation	922:940	The water contact angle, tensile strength, and modulus decreased slightly, but elongation at break increased after AgNPs incorporation.
26076636	8	20	theme	agar/AgNPs	1075:1084	arg1	film					1086:1089	The agar/AgNPs film	1071:1089	The agar/AgNPs film	1071:1089	The agar/AgNPs film could be applied to the active food packaging by controlling the food-borne pathogens.
26076636	5	21	from	change	710:715	arg1	structure					729:737	chemical structure	720:737	chemical structure	720:737	The incorporation of AgNPs into agar did not exhibit any change in chemical structure, thermal stability, moisture content, and water vapor permeability.
26076636	5	21	from	change	710:715	arg1	stability					748:756	thermal stability	740:756	thermal stability	740:756	The incorporation of AgNPs into agar did not exhibit any change in chemical structure, thermal stability, moisture content, and water vapor permeability.
26076636	5	21	from	change	710:715	arg1	content					768:774	moisture content	759:774	moisture content	759:774	The incorporation of AgNPs into agar did not exhibit any change in chemical structure, thermal stability, moisture content, and water vapor permeability.
26076636	5	21	from	change	710:715	arg1	permeability					793:804	water vapor permeability	781:804	water vapor permeability	781:804	The incorporation of AgNPs into agar did not exhibit any change in chemical structure, thermal stability, moisture content, and water vapor permeability.
26076636	7	22	theme	Listeria	1026:1033	arg1	monocytogenes					1035:1047	Listeria monocytogenes	1026:1047	Listeria monocytogenes	1026:1047	The agar/AgNPs nanocomposite films possessed strong antibacterial activity against Listeria monocytogenes and Escherichia coli.
26076636	2	23	theme	absorption	380:389	arg1	peak					391:394	characteristic absorption peak	365:394	characteristic absorption peak at 420 nm that showed a red shift to ∼434 nm after forming composite with agar	365:473	The AgNPs solutions exhibited characteristic absorption peak at 420 nm that showed a red shift to ∼434 nm after forming composite with agar.
26076636	0	24	theme	silver	33:38	arg1	nanoparticles					40:52	silver nanoparticles	33:52	silver nanoparticles	33:52	Amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films.
26076636	4	25	theme	agar	604:607	arg1	films					609:613	agar films	604:613	agar films	604:613	Apparent surface color and transmittance of agar films were greatly influenced by the AgNPs.
26076636	7	26	theme	antibacterial	995:1007	arg1	activity					1009:1016	strong antibacterial activity	988:1016	strong antibacterial activity	988:1016	The agar/AgNPs nanocomposite films possessed strong antibacterial activity against Listeria monocytogenes and Escherichia coli.
26076636	5	27	theme	moisture	759:766	arg1	content					768:774	moisture content	759:774	moisture content	759:774	The incorporation of AgNPs into agar did not exhibit any change in chemical structure, thermal stability, moisture content, and water vapor permeability.
26076636	8	28	theme	food-borne	1156:1165	arg1	pathogens					1167:1175	the food-borne pathogens	1152:1175	the food-borne pathogens	1152:1175	The agar/AgNPs film could be applied to the active food packaging by controlling the food-borne pathogens.
26076636	6	29	theme	contact	817:823	arg1	angle					825:829	The water contact angle	807:829	The water contact angle	807:829	The water contact angle, tensile strength, and modulus decreased slightly, but elongation at break increased after AgNPs incorporation.
26076636	2	30	theme	AgNPs	339:343	arg1	solutions					345:353	The AgNPs solutions	335:353	The AgNPs solutions	335:353	The AgNPs solutions exhibited characteristic absorption peak at 420 nm that showed a red shift to ∼434 nm after forming composite with agar.
26076636	3	31	theme	XRD	476:478	arg1	data					480:483	XRD data	476:483	XRD data	476:483	XRD data demonstrated the crystalline structure of AgNPs with dominant (111) facet.
26076636	0	32	theme	nanoparticles	40:52	arg1	preparation					58:68	preparation	58:68	preparation of antimicrobial agar/silver nanoparticles	58:111	Amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films.
26076636	0	32	theme	nanoparticles	40:52	arg1	synthesis					20:28	synthesis	20:28	synthesis of silver nanoparticles	20:52	Amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films.
26076636	1	33	theme	antimicrobial	304:316	arg1	films					328:332	antimicrobial composite films	304:332	antimicrobial composite films	304:332	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	6	34	theme	water	811:815	arg1	angle					825:829	The water contact angle	807:829	The water contact angle	807:829	The water contact angle, tensile strength, and modulus decreased slightly, but elongation at break increased after AgNPs incorporation.
26076636	1	35	dep	acids	188:192	arg1	tryptophan					208:217	tryptophan	208:217	tryptophan	208:217	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	1	35	dep	acids	188:192	arg1	tyrosine					195:202	tyrosine	195:202	tyrosine	195:202	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	1	35	dep	acids	188:192	arg1	acids					188:192	amino acids	182:192	amino acids (tyrosine and tryptophan)	182:218	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	2	36	from	nm	403:404	arg1	peak					391:394	characteristic absorption peak	365:394	characteristic absorption peak at 420 nm that showed a red shift to ∼434 nm after forming composite with agar	365:473	The AgNPs solutions exhibited characteristic absorption peak at 420 nm that showed a red shift to ∼434 nm after forming composite with agar.
26076636	1	37	theme	composite	318:326	arg1	films					328:332	antimicrobial composite films	304:332	antimicrobial composite films	304:332	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	8	38	theme	active	1115:1120	arg1	packaging					1127:1135	the active food packaging	1111:1135	the active food packaging	1111:1135	The agar/AgNPs film could be applied to the active food packaging by controlling the food-borne pathogens.
26076636	0	39	dep	synthesis	20:28	arg1	films					123:127	composite films	113:127	composite films	113:127	Amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films.
26076636	7	40	theme	strong	988:993	arg1	activity					1009:1016	strong antibacterial activity	988:1016	strong antibacterial activity	988:1016	The agar/AgNPs nanocomposite films possessed strong antibacterial activity against Listeria monocytogenes and Escherichia coli.
26076636	1	41	theme	reducing	223:230	arg1	agents					244:249	reducing and capping agents	223:249	reducing and capping agents	223:249	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	1	41	theme	reducing	223:230	arg1	acids					188:192	amino acids	182:192	amino acids (tyrosine and tryptophan)	182:218	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	5	42	theme	AgNPs	674:678	arg1	incorporation					657:669	The incorporation	653:669	The incorporation of AgNPs into agar	653:688	The incorporation of AgNPs into agar did not exhibit any change in chemical structure, thermal stability, moisture content, and water vapor permeability.
26076636	7	43	theme	nanocomposite	958:970	arg1	films					972:976	The agar/AgNPs nanocomposite films	943:976	The agar/AgNPs nanocomposite films	943:976	The agar/AgNPs nanocomposite films possessed strong antibacterial activity against Listeria monocytogenes and Escherichia coli.
26076636	5	44	theme	chemical	720:727	arg1	structure					729:737	chemical structure	720:737	chemical structure	720:737	The incorporation of AgNPs into agar did not exhibit any change in chemical structure, thermal stability, moisture content, and water vapor permeability.
26076636	3	45	theme	crystalline	502:512	arg1	structure					514:522	the crystalline structure	498:522	the crystalline structure of AgNPs	498:531	XRD data demonstrated the crystalline structure of AgNPs with dominant (111) facet.
26076636	1	46	theme	capping	236:242	arg1	agents					244:249	reducing and capping agents	223:249	reducing and capping agents	223:249	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	1	46	theme	capping	236:242	arg1	acids					188:192	amino acids	182:192	amino acids (tyrosine and tryptophan)	182:218	Silver nanoparticles (AgNPs) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films.
26076636	8	47	theme	food	1122:1125	arg1	packaging					1127:1135	the active food packaging	1111:1135	the active food packaging	1111:1135	The agar/AgNPs film could be applied to the active food packaging by controlling the food-borne pathogens.
26076636	2	48	theme	characteristic	365:378	arg1	peak					391:394	characteristic absorption peak	365:394	characteristic absorption peak at 420 nm that showed a red shift to ∼434 nm after forming composite with agar	365:473	The AgNPs solutions exhibited characteristic absorption peak at 420 nm that showed a red shift to ∼434 nm after forming composite with agar.
26076636	7	49	contain	possessed	978:986	arg2	activity					1009:1016	strong antibacterial activity	988:1016	strong antibacterial activity	988:1016	The agar/AgNPs nanocomposite films possessed strong antibacterial activity against Listeria monocytogenes and Escherichia coli.
26076636	7	49	contain	possessed	978:986	arg1	films					972:976	The agar/AgNPs nanocomposite films	943:976	The agar/AgNPs nanocomposite films	943:976	The agar/AgNPs nanocomposite films possessed strong antibacterial activity against Listeria monocytogenes and Escherichia coli.
28073678	9	0	theme	effective	1391:1399	arg1	compositions					1401:1412	the effective compositions	1387:1412	the effective compositions	1387:1412	Therefore, the effective compositions might be responsible for the significant neuroinflammation inhibitory activities exhibited by the herb.
28073678	9	0	theme	effective	1391:1399	arg1	responsible					1423:1433	responsible	1423:1433	responsible	1423:1433	Therefore, the effective compositions might be responsible for the significant neuroinflammation inhibitory activities exhibited by the herb.
28073678	7	1	theme	obvious	904:910	arg1	cytotoxicities					912:925	obvious cytotoxicities	904:925	obvious cytotoxicities	904:925	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	2	2	theme	previous	173:180	arg1	work					182:185	our previous work	169:185	our previous work on natural effective neuroinflammatory inhibitors	169:235	In our previous work on natural effective neuroinflammatory inhibitors, Alhagi sparsifolia Shap.
28073678	7	3	theme	inhibitory	874:883	arg1	activities					885:894	significant inhibitory activities	862:894	significant inhibitory activities	862:894	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	10	4	theme	therapeutic	1613:1623	arg1	agents					1625:1630	potential therapeutic agents	1603:1630	potential therapeutic agents against neurodegenerative diseases	1603:1665	Moreover, compounds 16 and 33 could be good leading compounds for the development of potential therapeutic agents against neurodegenerative diseases.
28073678	10	5	theme	neurodegenerative	1640:1656	arg1	diseases					1658:1665	neurodegenerative diseases	1640:1665	neurodegenerative diseases	1640:1665	Moreover, compounds 16 and 33 could be good leading compounds for the development of potential therapeutic agents against neurodegenerative diseases.
28073678	3	6	theme	anti-neuroinflammatory	377:398	arg1	effect					400:405	its significant anti-neuroinflammatory effect	361:405	its significant anti-neuroinflammatory effect	361:405	(Leguminosae), a folk medicine widely distributed in Xinjiang, attracted our attention because of its significant anti-neuroinflammatory effect.
28073678	6	7	theme	extract	667:673	arg1	effects					652:658	the anti-neuroinflammatory effects	625:658	the anti-neuroinflammatory effects of the extract	625:673	Furthermore, the anti-neuroinflammatory effects of the extract and purified constituents were evaluated in LPS-induced N9 cells in vitro.
28073678	6	7	theme	extract	667:673	arg1	constituents					688:699	purified constituents	679:699	purified constituents	679:699	Furthermore, the anti-neuroinflammatory effects of the extract and purified constituents were evaluated in LPS-induced N9 cells in vitro.
28073678	0	8	from	Shap	71:74	arg1	inhibitors					36:45	inhibitors	36:45	inhibitors	36:45	Natural potential neuroinflammatory inhibitors from Alhagi sparsifolia Shap.
28073678	4	9	theme	bioactive	448:456	arg1	basis					467:471	the bioactive material basis	444:471	the bioactive material basis	444:471	Therefore, further investigation of the bioactive material basis was carried out.
28073678	6	10	theme	purified	679:686	arg1	constituents					688:699	purified constituents	679:699	purified constituents	679:699	Furthermore, the anti-neuroinflammatory effects of the extract and purified constituents were evaluated in LPS-induced N9 cells in vitro.
28073678	3	11	theme	significant	365:375	arg1	effect					400:405	its significant anti-neuroinflammatory effect	361:405	its significant anti-neuroinflammatory effect	361:405	(Leguminosae), a folk medicine widely distributed in Xinjiang, attracted our attention because of its significant anti-neuroinflammatory effect.
28073678	8	12	theme	stronger	1292:1299	arg1	effect					1312:1317	much stronger inhibiting effect	1287:1317	much stronger inhibiting effect	1287:1317	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	8	13	theme	IC50	992:995	arg1	isorhamnetin					974:985	isorhamnetin (1)	974:989	isorhamnetin (1) (IC50 17.87μM)	974:1004	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	8	13	theme	IC50	992:995	arg1	17.87μM					997:1003	IC50 17.87μM	992:1003	IC50 17.87μM	992:1003	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	1	14	theme	key	100:102	arg1	contributor					104:114	a key contributor	98:114	a key contributor to neuronal damage in neurodegenerative diseases	98:163	Neuroinflammation is a key contributor to neuronal damage in neurodegenerative diseases.
28073678	1	14	theme	key	100:102	arg1	Neuroinflammation					77:93	Neuroinflammation	77:93	Neuroinflammation	77:93	Neuroinflammation is a key contributor to neuronal damage in neurodegenerative diseases.
28073678	9	15	theme	significant	1443:1453	arg1	activities					1484:1493	the significant neuroinflammation inhibitory activities	1439:1493	the significant neuroinflammation inhibitory activities exhibited by the herb	1439:1515	Therefore, the effective compositions might be responsible for the significant neuroinflammation inhibitory activities exhibited by the herb.
28073678	1	16	from	damage	128:133	arg1	diseases					156:163	neurodegenerative diseases	138:163	neurodegenerative diseases	138:163	Neuroinflammation is a key contributor to neuronal damage in neurodegenerative diseases.
28073678	8	17	theme	bombasinol	1173:1182	arg1	7.61μM					1192:1197	7.61μM	1192:1197	7.61μM	1192:1197	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	8	17	theme	bombasinol	1173:1182	arg1	A					1184:1184	bombasinol A	1173:1184	bombasinol A (17) (7.61μM)	1173:1198	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	8	17	theme	bombasinol	1173:1182	arg1	17					1187:1188	17	1187:1188	17	1187:1188	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	4	18	theme	further	419:425	arg1	investigation					427:439	further investigation	419:439	further investigation of the bioactive material basis	419:471	Therefore, further investigation of the bioactive material basis was carried out.
28073678	9	19	theme	neuroinflammation	1455:1471	arg1	activities					1484:1493	the significant neuroinflammation inhibitory activities	1439:1493	the significant neuroinflammation inhibitory activities exhibited by the herb	1439:1515	Therefore, the effective compositions might be responsible for the significant neuroinflammation inhibitory activities exhibited by the herb.
28073678	7	20	theme	significant	862:872	arg1	activities					885:894	significant inhibitory activities	862:894	significant inhibitory activities	862:894	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	2	21	dep	Shap	257:260	arg1	work					182:185	our previous work	169:185	our previous work on natural effective neuroinflammatory inhibitors	169:235	In our previous work on natural effective neuroinflammatory inhibitors, Alhagi sparsifolia Shap.
28073678	6	22	theme	anti-neuroinflammatory	629:650	arg1	effects					652:658	the anti-neuroinflammatory effects	625:658	the anti-neuroinflammatory effects of the extract	625:673	Furthermore, the anti-neuroinflammatory effects of the extract and purified constituents were evaluated in LPS-induced N9 cells in vitro.
28073678	9	23	theme	inhibitory	1473:1482	arg1	activities					1484:1493	the significant neuroinflammation inhibitory activities	1439:1493	the significant neuroinflammation inhibitory activities exhibited by the herb	1439:1515	Therefore, the effective compositions might be responsible for the significant neuroinflammation inhibitory activities exhibited by the herb.
28073678	2	24	theme	effective	198:206	arg1	inhibitors					226:235	natural effective neuroinflammatory inhibitors	190:235	natural effective neuroinflammatory inhibitors	190:235	In our previous work on natural effective neuroinflammatory inhibitors, Alhagi sparsifolia Shap.
28073678	1	25	theme	neuronal	119:126	arg1	damage					128:133	neuronal damage	119:133	neuronal damage in neurodegenerative diseases	119:163	Neuroinflammation is a key contributor to neuronal damage in neurodegenerative diseases.
28073678	3	26	theme	folk	280:283	arg1	medicine					285:292	a folk medicine	278:292	a folk medicine widely distributed in Xinjiang	278:323	(Leguminosae), a folk medicine widely distributed in Xinjiang, attracted our attention because of its significant anti-neuroinflammatory effect.
28073678	3	26	theme	folk	280:283	arg1	Leguminosae					264:274	(Leguminosae)	263:275	(Leguminosae)	263:275	(Leguminosae), a folk medicine widely distributed in Xinjiang, attracted our attention because of its significant anti-neuroinflammatory effect.
28073678	2	27	theme	natural	190:196	arg1	inhibitors					226:235	natural effective neuroinflammatory inhibitors	190:235	natural effective neuroinflammatory inhibitors	190:235	In our previous work on natural effective neuroinflammatory inhibitors, Alhagi sparsifolia Shap.
28073678	10	28	theme	good	1557:1560	arg1	compounds					1570:1578	good leading compounds	1557:1578	good leading compounds for the development of potential therapeutic agents against neurodegenerative diseases	1557:1665	Moreover, compounds 16 and 33 could be good leading compounds for the development of potential therapeutic agents against neurodegenerative diseases.
28073678	6	29	theme	N9	731:732	arg1	cells					734:738	LPS-induced N9 cells	719:738	LPS-induced N9 cells	719:738	Furthermore, the anti-neuroinflammatory effects of the extract and purified constituents were evaluated in LPS-induced N9 cells in vitro.
28073678	10	30	theme	agents	1625:1630	arg1	development					1588:1598	the development	1584:1598	the development of potential therapeutic agents against neurodegenerative diseases	1584:1665	Moreover, compounds 16 and 33 could be good leading compounds for the development of potential therapeutic agents against neurodegenerative diseases.
28073678	5	31	theme	spectral	580:587	arg1	methods					589:595	chromatographic and spectral methods	560:595	chromatographic and spectral methods	560:595	As a result, 33 major components were characterized and identified by chromatographic and spectral methods, respectively.
28073678	8	32	theme	1,3,3,4-tetramethyl	1233:1251	arg1	33					1267:1268	33	1267:1268	33	1267:1268	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	8	32	theme	1,3,3,4-tetramethyl	1233:1251	arg1	2.63μM					1272:1277	2.63μM	1272:1277	2.63μM	1272:1277	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	8	32	theme	1,3,3,4-tetramethyl	1233:1251	arg1	cyclopentene					1253:1264	1,3,3,4-tetramethyl cyclopentene	1233:1264	1,3,3,4-tetramethyl cyclopentene (33) (2.63μM)	1233:1278	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	8	33	theme	inhibiting	1301:1310	arg1	effect					1312:1317	much stronger inhibiting effect	1287:1317	much stronger inhibiting effect	1287:1317	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	10	34	theme	leading	1562:1568	arg1	compounds					1570:1578	good leading compounds	1557:1578	good leading compounds for the development of potential therapeutic agents against neurodegenerative diseases	1557:1665	Moreover, compounds 16 and 33 could be good leading compounds for the development of potential therapeutic agents against neurodegenerative diseases.
28073678	8	35	theme	control	1345:1351	arg1	19.89μM					1366:1372	19.89μM	1366:1372	19.89μM	1366:1372	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	8	35	theme	control	1345:1351	arg1	minocycline					1353:1363	the positive control minocycline	1332:1363	the positive control minocycline (19.89μM)	1332:1373	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	4	36	theme	basis	467:471	arg1	investigation					427:439	further investigation	419:439	further investigation of the bioactive material basis	419:471	Therefore, further investigation of the bioactive material basis was carried out.
28073678	7	37	dep	compounds	777:785	arg1	6					799:799	6	799:799	6	799:799	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	7	37	dep	compounds	777:785	arg1	15					809:810	15	809:810	15	809:810	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	7	37	dep	compounds	777:785	arg1	2					790:790	2	790:790	2	790:790	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	7	37	dep	compounds	777:785	arg1	3					793:793	3	793:793	3	793:793	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	7	37	dep	compounds	777:785	arg1	8					802:802	8	802:802	8	802:802	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	7	37	dep	compounds	777:785	arg1	11					805:806	11	805:806	11	805:806	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	7	37	dep	compounds	777:785	arg1	1					787:787	1	787:787	1	787:787	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	7	37	dep	compounds	777:785	arg1	5					796:796	5	796:796	5	796:796	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	6	38	theme	LPS-induced	719:729	arg1	cells					734:738	LPS-induced N9 cells	719:738	LPS-induced N9 cells	719:738	Furthermore, the anti-neuroinflammatory effects of the extract and purified constituents were evaluated in LPS-induced N9 cells in vitro.
28073678	8	39	theme	positive	1336:1343	arg1	19.89μM					1366:1372	19.89μM	1366:1372	19.89μM	1366:1372	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	8	39	theme	positive	1336:1343	arg1	minocycline					1353:1363	the positive control minocycline	1332:1363	the positive control minocycline (19.89μM)	1332:1373	Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) and 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than that of the positive control minocycline (19.89μM).
28073678	4	40	theme	material	458:465	arg1	basis					467:471	the bioactive material basis	444:471	the bioactive material basis	444:471	Therefore, further investigation of the bioactive material basis was carried out.
28073678	10	41	theme	potential	1603:1611	arg1	agents					1625:1630	potential therapeutic agents	1603:1630	potential therapeutic agents against neurodegenerative diseases	1603:1665	Moreover, compounds 16 and 33 could be good leading compounds for the development of potential therapeutic agents against neurodegenerative diseases.
28073678	7	42	theme	effective	936:944	arg1	concentrations					946:959	their effective concentrations	930:959	their effective concentrations	930:959	The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, 33 could exhibit significant inhibitory activities without obvious cytotoxicities at their effective concentrations.
28073678	1	43	theme	neurodegenerative	138:154	arg1	diseases					156:163	neurodegenerative diseases	138:163	neurodegenerative diseases	138:163	Neuroinflammation is a key contributor to neuronal damage in neurodegenerative diseases.
28073678	2	44	theme	neuroinflammatory	208:224	arg1	inhibitors					226:235	natural effective neuroinflammatory inhibitors	190:235	natural effective neuroinflammatory inhibitors	190:235	In our previous work on natural effective neuroinflammatory inhibitors, Alhagi sparsifolia Shap.
28073678	2	45	from	work	182:185	arg1	inhibitors					226:235	natural effective neuroinflammatory inhibitors	190:235	natural effective neuroinflammatory inhibitors	190:235	In our previous work on natural effective neuroinflammatory inhibitors, Alhagi sparsifolia Shap.
28073678	5	46	theme	major	506:510	arg1	result					495:500	a result	493:500	a result	493:500	As a result, 33 major components were characterized and identified by chromatographic and spectral methods, respectively.
28073678	5	46	theme	major	506:510	arg1	components					512:521	33 major components	503:521	33 major components	503:521	As a result, 33 major components were characterized and identified by chromatographic and spectral methods, respectively.
28073678	5	47	theme	chromatographic	560:574	arg1	methods					589:595	chromatographic and spectral methods	560:595	chromatographic and spectral methods	560:595	As a result, 33 major components were characterized and identified by chromatographic and spectral methods, respectively.
27711252	2	0	theme	carboxy-terminal	326:341	arg1	domain					343:348	a conserved carboxy-terminal domain	314:348	a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export	314:408	The secreted proteins have a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export.
27711252	10	1	theme	periplasmic	1734:1744	arg1	side					1746:1749	the periplasmic side	1730:1749	the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions	1730:1841	PG1058 is a predicted lipoprotein and was localised to the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions.
27711252	4	2	from	T9SS	650:653	arg1	gingivalis					661:670	P. gingivalis	658:670	P. gingivalis	658:670	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	1	3	theme	virulence	245:253	arg1	factors					255:261	virulence factors	245:261	virulence factors such as the gingipains	245:284	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	1	3	theme	virulence	245:253	arg1	gingipains					275:284	the gingipains	271:284	the gingipains	271:284	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	5	4	theme	structure	731:739	arg1	modelling					707:715	Computer modelling	698:715	Computer modelling of the PG1058 structure	698:739	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	7	5	theme	LC-MS/MS	1157:1164	arg1	analyses					1166:1173	Immunoblot and LC-MS/MS analyses	1142:1173	Immunoblot and LC-MS/MS analyses of subcellular fractions	1142:1198	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	9	6	theme	A-LPS	1668:1672	arg1	translocation					1651:1663	the translocation	1647:1663	the translocation of A-LPS	1647:1672	This indicated that PG1058 is crucial for export of T9SS substrates but not for the translocation of A-LPS.
27711252	4	7	theme	T9SS	650:653	arg1	components					632:641	ten putative novel components	613:641	ten putative novel components	613:641	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	10	8	theme	OM	1758:1759	arg1	side					1746:1749	the periplasmic side	1730:1749	the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions	1730:1841	PG1058 is a predicted lipoprotein and was localised to the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions.
27711252	11	9	theme	T9SS	2004:2007	arg1	components					1986:1995	the periplasmic and OM components	1963:1995	the periplasmic and OM components of the T9SS	1963:2007	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	5	10	theme	tetratricopeptide	802:818	arg1	repeat					820:825	a tetratricopeptide repeat	800:825	a tetratricopeptide repeat (TPR) domain	800:838	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	5	10	theme	tetratricopeptide	802:818	arg1	TPR					828:830	TPR	828:830	TPR	828:830	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	11	11	theme	PG1058	1890:1895	arg1	prediction					1859:1868	structural prediction	1848:1868	structural prediction	1848:1868	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	11	11	theme	PG1058	1890:1895	arg1	localisation					1874:1885	localisation	1874:1885	localisation	1874:1885	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	4	12	theme	novel	626:630	arg1	components					632:641	ten putative novel components	613:641	ten putative novel components	613:641	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	11	13	theme	essential	1936:1944	arg1	role					1925:1928	a role	1923:1928	a role	1923:1928	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	11	13	theme	essential	1936:1944	arg1	scaffold					1946:1953	an essential scaffold	1933:1953	an essential scaffold linking the periplasmic and OM components of the T9SS	1933:2007	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	7	14	theme	perturbed	1366:1374	arg1	function					1381:1388	perturbed T9SS function	1366:1388	perturbed T9SS function	1366:1388	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	10	15	theme	whole-cell	1767:1776	arg1	ELISA					1778:1782	whole-cell ELISA	1767:1782	whole-cell ELISA	1767:1782	PG1058 is a predicted lipoprotein and was localised to the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions.
27711252	5	16	theme	carboxypeptidase	865:880	arg1	domain					855:860	a β-propeller domain	841:860	a β-propeller domain	841:860	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	5	16	theme	carboxypeptidase	865:880	arg1	fold					905:908	a carboxypeptidase regulatory domain-like fold	863:908	a carboxypeptidase regulatory domain-like fold	863:908	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	11	17	theme	periplasmic	1967:1977	arg1	components					1986:1995	the periplasmic and OM components	1963:1995	the periplasmic and OM components of the T9SS	1963:2007	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	5	18	theme	domain-like	893:903	arg1	domain					855:860	a β-propeller domain	841:860	a β-propeller domain	841:860	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	5	18	theme	domain-like	893:903	arg1	fold					905:908	a carboxypeptidase regulatory domain-like fold	863:908	a carboxypeptidase regulatory domain-like fold	863:908	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	6	19	theme	pg1058	990:995	arg1	Inactivation					974:985	Inactivation	974:985	Inactivation of pg1058 in P. gingivalis	974:1012	Inactivation of pg1058 in P. gingivalis resulted in loss of both colonial pigmentation and surface-associated proteolytic activity; a phenotype common to T9SS mutants.
27711252	7	20	theme	cell	1341:1344	arg1	surface					1346:1352	the cell surface	1337:1352	the cell surface	1337:1352	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	4	21	theme	P.	658:659	arg1	gingivalis					661:670	P. gingivalis	658:670	P. gingivalis	658:670	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	5	22	theme	β-propeller	843:853	arg1	domain					855:860	a β-propeller domain	841:860	a β-propeller domain	841:860	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	5	22	theme	β-propeller	843:853	arg1	CRD					911:913	CRD	911:913	CRD	911:913	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	5	22	theme	β-propeller	843:853	arg1	fold					905:908	a carboxypeptidase regulatory domain-like fold	863:908	a carboxypeptidase regulatory domain-like fold	863:908	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	8	23	theme	mutant	1492:1497	arg1	surface					1504:1510	the pg1058 mutant cell surface	1481:1510	the pg1058 mutant cell surface	1481:1510	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	0	24	theme	Type	65:68	arg1	System					83:88	the Bacterial Type IX Secretion System	51:88	the Bacterial Type IX Secretion System	51:88	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	0	25	theme	Secretion	73:81	arg1	System					83:88	the Bacterial Type IX Secretion System	51:88	the Bacterial Type IX Secretion System	51:88	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	11	26	contain	have	1918:1921	arg2	role					1925:1928	a role	1923:1928	a role	1923:1928	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	11	26	contain	have	1918:1921	arg2	scaffold					1946:1953	an essential scaffold	1933:1953	an essential scaffold linking the periplasmic and OM components of the T9SS	1933:2007	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	11	26	contain	have	1918:1921	arg1	it					1911:1912	it	1911:1912	it	1911:1912	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	1	27	theme	Bacteroidetes-specific	129:150	arg1	T9SS					178:181	T9SS	178:181	T9SS	178:181	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	1	27	theme	Bacteroidetes-specific	129:150	arg1	system					170:175	the Bacteroidetes-specific type IX secretion system	125:175	the Bacteroidetes-specific type IX secretion system (T9SS)	125:182	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	3	28	theme	P.	414:415	arg1	gingivalis					417:426	P. gingivalis	414:426	P. gingivalis	414:426	In P. gingivalis the T9SS substrates undergo glycosylation with anionic lipopolysaccharide (A-LPS) and are attached to the OM.
27711252	5	29	theme	putative	935:942	arg1	domain					966:971	an OmpA_C-like putative peptidoglycan binding domain	920:971	an OmpA_C-like putative peptidoglycan binding domain	920:971	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	2	30	theme	IX	369:370	arg1	secretion					372:380	type IX secretion	364:380	type IX secretion that is cleaved upon export	364:408	The secreted proteins have a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export.
27711252	5	31	theme	C-terminal	765:774	arg1	architecture					776:787	C-terminal architecture	765:787	C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain	765:971	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	11	32	dep	prediction	1859:1868	arg1	The					1844:1846	The	1844:1846	The	1844:1846	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	7	33	theme	Immunoblot	1142:1151	arg1	analyses					1166:1173	Immunoblot and LC-MS/MS analyses	1142:1173	Immunoblot and LC-MS/MS analyses of subcellular fractions	1142:1198	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	1	34	theme	Porphyromonas	91:103	arg1	gingivalis					105:114	Porphyromonas gingivalis	91:114	Porphyromonas gingivalis	91:114	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	3	35	attach	attached	518:525	arg2	substrates					437:446	the T9SS substrates	428:446	the T9SS substrates	428:446	In P. gingivalis the T9SS substrates undergo glycosylation with anionic lipopolysaccharide (A-LPS) and are attached to the OM.
27711252	3	35	attach	attached	518:525	arg1	OM					534:535	the OM	530:535	the OM	530:535	In P. gingivalis the T9SS substrates undergo glycosylation with anionic lipopolysaccharide (A-LPS) and are attached to the OM.
27711252	8	36	theme	substrate	1547:1555	arg1	proteins					1557:1564	T9SS substrate proteins	1542:1564	T9SS substrate proteins	1542:1564	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	0	37	theme	Multidomain	18:28	arg1	PG1058					0:5	PG1058	0:5	PG1058	0:5	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	0	37	theme	Multidomain	18:28	arg1	Component					38:46	a Novel Multidomain Protein Component	10:46	a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System	10:88	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	6	38	theme	P.	1000:1001	arg1	gingivalis					1003:1012	P. gingivalis	1000:1012	P. gingivalis	1000:1012	Inactivation of pg1058 in P. gingivalis resulted in loss of both colonial pigmentation and surface-associated proteolytic activity; a phenotype common to T9SS mutants.
27711252	10	39	theme	subcellular	1821:1831	arg1	fractions					1833:1841	subcellular fractions	1821:1841	subcellular fractions	1821:1841	PG1058 is a predicted lipoprotein and was localised to the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions.
27711252	3	40	theme	anionic	475:481	arg1	A-LPS					503:507	A-LPS	503:507	A-LPS	503:507	In P. gingivalis the T9SS substrates undergo glycosylation with anionic lipopolysaccharide (A-LPS) and are attached to the OM.
27711252	3	40	theme	anionic	475:481	arg1	lipopolysaccharide					483:500	anionic lipopolysaccharide	475:500	anionic lipopolysaccharide (A-LPS)	475:508	In P. gingivalis the T9SS substrates undergo glycosylation with anionic lipopolysaccharide (A-LPS) and are attached to the OM.
27711252	8	41	theme	ELISA	1422:1426	arg1	analyses					1428:1435	Immunoblot, TEM and whole-cell ELISA analyses	1391:1435	Immunoblot, TEM and whole-cell ELISA analyses	1391:1435	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	1	42	theme	outer	214:218	arg1	OM					230:231	OM	230:231	OM	230:231	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	1	42	theme	outer	214:218	arg1	membrane					220:227	the outer membrane	210:227	the outer membrane (OM)	210:232	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	11	43	theme	OM	1983:1984	arg1	components					1986:1995	the periplasmic and OM components	1963:1995	the periplasmic and OM components of the T9SS	1963:2007	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	5	44	theme	binding	958:964	arg1	domain					966:971	an OmpA_C-like putative peptidoglycan binding domain	920:971	an OmpA_C-like putative peptidoglycan binding domain	920:971	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	6	45	theme	activity	1096:1103	arg1	loss					1026:1029	loss	1026:1029	loss of both colonial pigmentation and surface-associated proteolytic activity	1026:1103	Inactivation of pg1058 in P. gingivalis resulted in loss of both colonial pigmentation and surface-associated proteolytic activity; a phenotype common to T9SS mutants.
27711252	9	46	theme	substrates	1624:1633	arg1	export					1609:1614	export	1609:1614	export of T9SS substrates	1609:1633	This indicated that PG1058 is crucial for export of T9SS substrates but not for the translocation of A-LPS.
27711252	6	47	theme	surface-associated	1065:1082	arg1	activity					1096:1103	surface-associated proteolytic activity	1065:1103	surface-associated proteolytic activity	1065:1103	Inactivation of pg1058 in P. gingivalis resulted in loss of both colonial pigmentation and surface-associated proteolytic activity; a phenotype common to T9SS mutants.
27711252	4	48	theme	Bacteroidetes	580:592	arg1	genomes					594:600	24 Bacteroidetes genomes	577:600	24 Bacteroidetes genomes	577:600	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	5	49	theme	PG1058	724:729	arg1	structure					731:739	the PG1058 structure	720:739	the PG1058 structure	720:739	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	11	50	theme	structural	1848:1857	arg1	prediction					1859:1868	structural prediction	1848:1868	structural prediction	1848:1868	The structural prediction and localisation of PG1058 suggests that it may have a role as an essential scaffold linking the periplasmic and OM components of the T9SS.
27711252	4	51	from	gingivalis	661:670	arg1	components					632:641	ten putative novel components	613:641	ten putative novel components	613:641	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	6	52	theme	pigmentation	1048:1059	arg1	loss					1026:1029	loss	1026:1029	loss of both colonial pigmentation and surface-associated proteolytic activity	1026:1103	Inactivation of pg1058 in P. gingivalis resulted in loss of both colonial pigmentation and surface-associated proteolytic activity; a phenotype common to T9SS mutants.
27711252	4	53	theme	comparative	553:563	arg1	analyses					565:572	comparative analyses	553:572	comparative analyses of 24 Bacteroidetes genomes	553:600	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	2	54	theme	conserved	316:324	arg1	domain					343:348	a conserved carboxy-terminal domain	314:348	a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export	314:408	The secreted proteins have a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export.
27711252	3	55	with	glycosylation	456:468	arg1	A-LPS					503:507	A-LPS	503:507	A-LPS	503:507	In P. gingivalis the T9SS substrates undergo glycosylation with anionic lipopolysaccharide (A-LPS) and are attached to the OM.
27711252	3	55	with	glycosylation	456:468	arg1	lipopolysaccharide					483:500	anionic lipopolysaccharide	475:500	anionic lipopolysaccharide (A-LPS)	475:508	In P. gingivalis the T9SS substrates undergo glycosylation with anionic lipopolysaccharide (A-LPS) and are attached to the OM.
27711252	4	56	theme	components	632:641	arg1	components					632:641	ten putative novel components	613:641	ten putative novel components	613:641	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	4	56	theme	components	632:641	arg1	one					673:675	one	673:675	one	673:675	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	7	57	theme	T9SS	1209:1212	arg1	substrates					1214:1223	T9SS substrates	1209:1223	T9SS substrates accumulated within the pg1058 mutant periplasm	1209:1270	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	2	58	theme	secreted	291:298	arg1	proteins					300:307	The secreted proteins	287:307	The secreted proteins	287:307	The secreted proteins have a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export.
27711252	4	59	from	components	632:641	arg1	gingivalis					661:670	P. gingivalis	658:670	P. gingivalis	658:670	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	6	60	theme	T9SS	1128:1131	arg1	mutants					1133:1139	T9SS mutants	1128:1139	T9SS mutants	1128:1139	Inactivation of pg1058 in P. gingivalis resulted in loss of both colonial pigmentation and surface-associated proteolytic activity; a phenotype common to T9SS mutants.
27711252	8	61	theme	TEM	1403:1405	arg1	analyses					1428:1435	Immunoblot, TEM and whole-cell ELISA analyses	1391:1435	Immunoblot, TEM and whole-cell ELISA analyses	1391:1435	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	1	62	theme	export	187:192	arg1	proteins					194:201	export proteins	187:201	export proteins	187:201	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	1	62	theme	export	187:192	arg1	factors					255:261	virulence factors	245:261	virulence factors such as the gingipains	245:284	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	6	63	theme	common	1118:1123	arg1	phenotype					1108:1116	a phenotype	1106:1116	a phenotype common to T9SS mutants	1106:1139	Inactivation of pg1058 in P. gingivalis resulted in loss of both colonial pigmentation and surface-associated proteolytic activity; a phenotype common to T9SS mutants.
27711252	8	64	attach	linked	1532:1537	arg1	proteins					1557:1564	T9SS substrate proteins	1542:1564	T9SS substrate proteins	1542:1564	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	8	64	attach	linked	1532:1537	arg2	it					1521:1522	it	1521:1522	it	1521:1522	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	5	65	theme	repeat	820:825	arg1	domain					833:838	a tetratricopeptide repeat (TPR) domain	800:838	a tetratricopeptide repeat (TPR) domain	800:838	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	4	66	theme	putative	617:624	arg1	components					632:641	ten putative novel components	613:641	ten putative novel components	613:641	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	7	67	theme	subcellular	1178:1188	arg1	fractions					1190:1198	subcellular fractions	1178:1198	subcellular fractions	1178:1198	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	8	68	dep	indicated	1437:1445	arg1	produced					1457:1464	produced	1457:1464	was produced	1453:1464	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	8	68	dep	indicated	1437:1445	arg1	present					1470:1476	present	1470:1476	present	1470:1476	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	7	69	theme	T9SS	1376:1379	arg1	function					1381:1388	perturbed T9SS function	1366:1388	perturbed T9SS function	1366:1388	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	5	70	theme	peptidoglycan	944:956	arg1	domain					966:971	an OmpA_C-like putative peptidoglycan binding domain	920:971	an OmpA_C-like putative peptidoglycan binding domain	920:971	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	5	71	theme	regulatory	882:891	arg1	domain					855:860	a β-propeller domain	841:860	a β-propeller domain	841:860	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	5	71	theme	regulatory	882:891	arg1	fold					905:908	a carboxypeptidase regulatory domain-like fold	863:908	a carboxypeptidase regulatory domain-like fold	863:908	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	8	72	theme	pg1058	1485:1490	arg1	surface					1504:1510	the pg1058 mutant cell surface	1481:1510	the pg1058 mutant cell surface	1481:1510	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	0	73	theme	Bacterial	55:63	arg1	System					83:88	the Bacterial Type IX Secretion System	51:88	the Bacterial Type IX Secretion System	51:88	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	10	74	theme	LC-MS/MS	1800:1807	arg1	analyses					1809:1816	LC-MS/MS analyses	1800:1816	LC-MS/MS analyses	1800:1816	PG1058 is a predicted lipoprotein and was localised to the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions.
27711252	1	75	theme	type	152:155	arg1	T9SS					178:181	T9SS	178:181	T9SS	178:181	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	1	75	theme	type	152:155	arg1	system					170:175	the Bacteroidetes-specific type IX secretion system	125:175	the Bacteroidetes-specific type IX secretion system (T9SS)	125:182	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	8	76	theme	cell	1499:1502	arg1	surface					1504:1510	the pg1058 mutant cell surface	1481:1510	the pg1058 mutant cell surface	1481:1510	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	7	77	theme	fractions	1190:1198	arg1	analyses					1166:1173	Immunoblot and LC-MS/MS analyses	1142:1173	Immunoblot and LC-MS/MS analyses of subcellular fractions	1142:1198	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	0	78	theme	IX	70:71	arg1	System					83:88	the Bacterial Type IX Secretion System	51:88	the Bacterial Type IX Secretion System	51:88	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	2	79	contain	have	309:312	arg1	proteins					300:307	The secreted proteins	287:307	The secreted proteins	287:307	The secreted proteins have a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export.
27711252	2	79	contain	have	309:312	arg2	domain					343:348	a conserved carboxy-terminal domain	314:348	a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export	314:408	The secreted proteins have a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export.
27711252	1	80	theme	secretion	160:168	arg1	T9SS					178:181	T9SS	178:181	T9SS	178:181	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	1	80	theme	secretion	160:168	arg1	system					170:175	the Bacteroidetes-specific type IX secretion system	125:175	the Bacteroidetes-specific type IX secretion system (T9SS)	125:182	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	6	81	from	Inactivation	974:985	arg1	gingivalis					1003:1012	P. gingivalis	1000:1012	P. gingivalis	1000:1012	Inactivation of pg1058 in P. gingivalis resulted in loss of both colonial pigmentation and surface-associated proteolytic activity; a phenotype common to T9SS mutants.
27711252	0	82	theme	System	83:88	arg1	PG1058					0:5	PG1058	0:5	PG1058	0:5	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	0	82	theme	System	83:88	arg1	Component					38:46	a Novel Multidomain Protein Component	10:46	a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System	10:88	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	10	83	theme	predicted	1687:1695	arg1	PG1058					1675:1680	PG1058	1675:1680	PG1058	1675:1680	PG1058 is a predicted lipoprotein and was localised to the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions.
27711252	10	83	theme	predicted	1687:1695	arg1	lipoprotein					1697:1707	a predicted lipoprotein	1685:1707	a predicted lipoprotein	1685:1707	PG1058 is a predicted lipoprotein and was localised to the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions.
27711252	5	84	theme	Computer	698:705	arg1	modelling					707:715	Computer modelling	698:715	Computer modelling of the PG1058 structure	698:739	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	5	85	theme	OmpA_C-like	923:933	arg1	domain					966:971	an OmpA_C-like putative peptidoglycan binding domain	920:971	an OmpA_C-like putative peptidoglycan binding domain	920:971	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	7	86	from	surface	1346:1352	arg1	absent					1325:1330	absent	1325:1330	absent	1325:1330	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	6	87	theme	colonial	1039:1046	arg1	pigmentation					1048:1059	colonial pigmentation	1039:1059	colonial pigmentation	1039:1059	Inactivation of pg1058 in P. gingivalis resulted in loss of both colonial pigmentation and surface-associated proteolytic activity; a phenotype common to T9SS mutants.
27711252	6	88	dep	resulted	1014:1021	arg1	phenotype					1108:1116	a phenotype	1106:1116	a phenotype common to T9SS mutants	1106:1139	Inactivation of pg1058 in P. gingivalis resulted in loss of both colonial pigmentation and surface-associated proteolytic activity; a phenotype common to T9SS mutants.
27711252	8	89	theme	T9SS	1542:1545	arg1	proteins					1557:1564	T9SS substrate proteins	1542:1564	T9SS substrate proteins	1542:1564	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	2	90	theme	type	364:367	arg1	secretion					372:380	type IX secretion	364:380	type IX secretion that is cleaved upon export	364:408	The secreted proteins have a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export.
27711252	0	91	theme	Novel	12:16	arg1	PG1058					0:5	PG1058	0:5	PG1058	0:5	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	0	91	theme	Novel	12:16	arg1	Component					38:46	a Novel Multidomain Protein Component	10:46	a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System	10:88	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	7	92	theme	Kgp	1307:1309	arg1	gingipain					1311:1319	the Kgp gingipain	1303:1319	the Kgp gingipain	1303:1319	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	3	93	gly	glycosylation	456:468	arg1	gingivalis					417:426	P. gingivalis	414:426	P. gingivalis	414:426	In P. gingivalis the T9SS substrates undergo glycosylation with anionic lipopolysaccharide (A-LPS) and are attached to the OM.
27711252	3	93	gly	glycosylation	456:468	arg1	substrates					437:446	the T9SS substrates	428:446	the T9SS substrates	428:446	In P. gingivalis the T9SS substrates undergo glycosylation with anionic lipopolysaccharide (A-LPS) and are attached to the OM.
27711252	6	94	theme	proteolytic	1084:1094	arg1	activity					1096:1103	surface-associated proteolytic activity	1065:1103	surface-associated proteolytic activity	1065:1103	Inactivation of pg1058 in P. gingivalis resulted in loss of both colonial pigmentation and surface-associated proteolytic activity; a phenotype common to T9SS mutants.
27711252	0	95	theme	Protein	30:36	arg1	PG1058					0:5	PG1058	0:5	PG1058	0:5	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	0	95	theme	Protein	30:36	arg1	Component					38:46	a Novel Multidomain Protein Component	10:46	a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System	10:88	PG1058 Is a Novel Multidomain Protein Component of the Bacterial Type IX Secretion System.
27711252	10	96	theme	fractions	1833:1841	arg1	immunoblot					1785:1794	immunoblot	1785:1794	immunoblot	1785:1794	PG1058 is a predicted lipoprotein and was localised to the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions.
27711252	10	96	theme	fractions	1833:1841	arg1	analyses					1809:1816	LC-MS/MS analyses	1800:1816	LC-MS/MS analyses	1800:1816	PG1058 is a predicted lipoprotein and was localised to the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions.
27711252	10	96	theme	fractions	1833:1841	arg1	ELISA					1778:1782	whole-cell ELISA	1767:1782	whole-cell ELISA	1767:1782	PG1058 is a predicted lipoprotein and was localised to the periplasmic side of the OM using whole-cell ELISA, immunoblot and LC-MS/MS analyses of subcellular fractions.
27711252	8	97	theme	Immunoblot	1391:1400	arg1	analyses					1428:1435	Immunoblot, TEM and whole-cell ELISA analyses	1391:1435	Immunoblot, TEM and whole-cell ELISA analyses	1391:1435	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	3	98	theme	T9SS	432:435	arg1	substrates					437:446	the T9SS substrates	428:446	the T9SS substrates	428:446	In P. gingivalis the T9SS substrates undergo glycosylation with anionic lipopolysaccharide (A-LPS) and are attached to the OM.
27711252	5	99	theme	novel	753:757	arg1	N-					759:760	a novel N-	751:760	a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain	751:971	Computer modelling of the PG1058 structure predicted a novel N- to C-terminal architecture comprising a tetratricopeptide repeat (TPR) domain, a β-propeller domain, a carboxypeptidase regulatory domain-like fold (CRD) and an OmpA_C-like putative peptidoglycan binding domain.
27711252	7	100	theme	whole-cell	1279:1288	arg1	ELISA					1290:1294	whole-cell ELISA	1279:1294	whole-cell ELISA	1279:1294	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	1	101	theme	IX	157:158	arg1	T9SS					178:181	T9SS	178:181	T9SS	178:181	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	1	101	theme	IX	157:158	arg1	system					170:175	the Bacteroidetes-specific type IX secretion system	125:175	the Bacteroidetes-specific type IX secretion system (T9SS)	125:182	Porphyromonas gingivalis utilises the Bacteroidetes-specific type IX secretion system (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.
27711252	7	102	dep	showed	1296:1301	arg1	absent					1325:1330	absent	1325:1330	absent	1325:1330	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	8	103	theme	whole-cell	1411:1420	arg1	ELISA					1422:1426	whole-cell ELISA	1411:1426	whole-cell ELISA	1411:1426	Immunoblot, TEM and whole-cell ELISA analyses indicated A-LPS was produced and present on the pg1058 mutant cell surface although it was not linked to T9SS substrate proteins.
27711252	7	104	theme	mutant	1255:1260	arg1	periplasm					1262:1270	the pg1058 mutant periplasm	1244:1270	the pg1058 mutant periplasm	1244:1270	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	9	105	theme	T9SS	1619:1622	arg1	substrates					1624:1633	T9SS substrates	1619:1633	T9SS substrates	1619:1633	This indicated that PG1058 is crucial for export of T9SS substrates but not for the translocation of A-LPS.
27711252	7	106	theme	pg1058	1248:1253	arg1	periplasm					1262:1270	the pg1058 mutant periplasm	1244:1270	the pg1058 mutant periplasm	1244:1270	Immunoblot and LC-MS/MS analyses of subcellular fractions revealed T9SS substrates accumulated within the pg1058 mutant periplasm whilst whole-cell ELISA showed the Kgp gingipain was absent from the cell surface, confirming perturbed T9SS function.
27711252	4	107	theme	genomes	594:600	arg1	analyses					565:572	comparative analyses	553:572	comparative analyses of 24 Bacteroidetes genomes	553:600	In this study, comparative analyses of 24 Bacteroidetes genomes identified ten putative novel components of the T9SS in P. gingivalis, one of which was PG1058.
27711252	2	108	theme	essential	350:358	arg1	domain					343:348	a conserved carboxy-terminal domain	314:348	a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export	314:408	The secreted proteins have a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export.
26586776	6	0	theme	wt/vol	929:934	arg1	nHA					937:939	1 wt/vol% nHA	927:939	1 wt/vol% nHA	927:939	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	6	0	theme	wt/vol	929:934	arg1	HCG-111					918:924	HCG-111	918:924	HCG-111 (1 wt/vol% nHA)	918:940	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	12	1	from	hGFs	1809:1812	arg1	differentiation					1778:1792	osteogenic differentiation	1767:1792	osteogenic differentiation of hiPSCs from hGFs	1767:1812	In conclusion, our study has shown that osteogenic differentiation of hiPSCs from hGFs was improved by HCG-311.
26586776	12	1	from	hGFs	1809:1812	arg1	hiPSCs					1797:1802	hiPSCs	1797:1802	hiPSCs from hGFs	1797:1812	In conclusion, our study has shown that osteogenic differentiation of hiPSCs from hGFs was improved by HCG-311.
26586776	19	2	from	role	2652:2655	arg1	scaffolds					2688:2696	HCG scaffolds	2684:2696	HCG scaffolds	2684:2696	The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs.
26586776	6	3	theme	nHA/chitosan/gelatin	846:865	arg1	HCG-311					946:952	HCG-311	946:952	HCG-311 (3 wt/vol% nHA)	946:968	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	6	3	theme	nHA/chitosan/gelatin	846:865	arg1	HCG-111					918:924	HCG-111	918:924	HCG-111 (1 wt/vol% nHA)	918:940	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	6	3	theme	nHA/chitosan/gelatin	846:865	arg1	scaffolds					873:881	nHA/chitosan/gelatin (HCG) scaffolds	846:881	nHA/chitosan/gelatin (HCG) scaffolds	846:881	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	1	4	theme	bone	289:292	arg1	engineering					301:311	bone tissue engineering	289:311	bone tissue engineering	289:311	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	12	5	theme	osteogenic	1767:1776	arg1	differentiation					1778:1792	osteogenic differentiation	1767:1792	osteogenic differentiation of hiPSCs from hGFs	1767:1812	In conclusion, our study has shown that osteogenic differentiation of hiPSCs from hGFs was improved by HCG-311.
26586776	11	6	theme	osteogenic-related	1417:1434	arg1	expression					1441:1450	osteogenic-related gene expression	1417:1450	osteogenic-related gene expression of hiPSCs	1417:1460	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	6	7	link	patient-derived	812:826	arg1	hGFs					828:831	patient-derived hGFs	812:831	patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA)	812:968	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	16	8	theme	human	2273:2277	arg1	hGFs					2301:2304	hGFs	2301:2304	hGFs	2301:2304	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	16	8	theme	human	2273:2277	arg1	fibroblasts					2288:2298	human gingival fibroblasts	2273:2298	clinically easily derived human gingival fibroblasts (hGFs)	2247:2305	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	6	9	theme	patient-derived	812:826	arg1	hGFs					828:831	patient-derived hGFs	812:831	patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA)	812:968	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	18	10	theme	hiPSCs	2501:2506	arg1	differentiation					2482:2496	osteogenic differentiation	2471:2496	osteogenic differentiation of hiPSCs from hGFs	2471:2516	More interestingly, osteogenic differentiation of hiPSCs from hGFs was elevated significantly when composited with HCG-311 scaffolds in vitro and in vivo.
26586776	16	11	theme	Human	2179:2183	arg1	hiPSCs					2217:2222	hiPSCs	2217:2222	hiPSCs	2217:2222	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	16	11	theme	Human	2179:2183	arg1	cells					2210:2214	Human induced pluripotent stem cells	2179:2214	Human induced pluripotent stem cells (hiPSCs)	2179:2223	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	5	12	theme	human	668:672	arg1	fibroblasts					683:693	human gingival fibroblasts	668:693	human gingival fibroblasts (hGFs)	668:700	However, its regulation on osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs) is unknown.
26586776	5	12	theme	human	668:672	arg1	hGFs					696:699	hGFs	696:699	hGFs	696:699	However, its regulation on osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs) is unknown.
26586776	6	13	theme	hiPSCs	800:805	arg1	differentiation					781:795	the osteogenic differentiation	766:795	the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA)	766:968	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	1	14	theme	adult	235:239	arg1	cells					246:250	adult stem cells	235:250	adult stem cells	235:250	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	14	theme	adult	235:239	arg1	source					278:283	the cell source	269:283	the cell source for bone tissue engineering	269:311	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	14	theme	adult	235:239	arg1	cells					225:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	19	15	theme	hiPSCs	2727:2732	arg1	induction					2714:2722	osteogenesis induction	2701:2722	osteogenesis induction of hiPSCs derived from hGFs	2701:2750	The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs.
26586776	6	16	theme	wt/vol	957:962	arg1	nHA					965:967	3 wt/vol% nHA	955:967	3 wt/vol% nHA	955:967	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	6	16	theme	wt/vol	957:962	arg1	HCG-311					946:952	HCG-311	946:952	HCG-311 (3 wt/vol% nHA)	946:968	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	11	17	dep	imaging	1640:1646	arg1	staining					1717:1724	OCN immunohistochemistry staining	1692:1724	OCN immunohistochemistry staining	1692:1724	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	11	17	dep	imaging	1640:1646	arg1	staining					1653:1660	H&E staining	1649:1660	H&E staining	1649:1660	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	11	17	dep	imaging	1640:1646	arg1	staining					1670:1677	Masson staining	1663:1677	Masson staining	1663:1677	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	11	17	dep	imaging	1640:1646	arg1	RUNX-2					1684:1689	RUNX-2	1684:1689	RUNX-2	1684:1689	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	16	18	theme	pluripotent	2193:2203	arg1	hiPSCs					2217:2222	hiPSCs	2217:2222	hiPSCs	2217:2222	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	16	18	theme	pluripotent	2193:2203	arg1	cells					2210:2214	Human induced pluripotent stem cells	2179:2214	Human induced pluripotent stem cells (hiPSCs)	2179:2223	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	7	19	theme	differentiation	1045:1059	arg1	capability					1061:1070	enhanced osteogenic differentiation capability	1025:1070	enhanced osteogenic differentiation capability	1025:1070	First, hGFs were reprogrammed into hiPSCs, which have enhanced osteogenic differentiation capability.
26586776	9	20	theme	immunocompromised	1230:1246	arg1	mice					1248:1251	immunocompromised mice	1230:1251	immunocompromised mice	1230:1251	Finally, hiPSC/HCG complexes were cultured in vitro or subcutaneously transplanted into immunocompromised mice in vivo.
26586776	13	21	theme	hiPSCs	1927:1932	arg1	expression					1913:1922	osteogenic marker expression	1895:1922	osteogenic marker expression of hiPSCs from hGFs	1895:1942	The mechanism might be that the nHA addition stimulates osteogenic marker expression of hiPSCs from hGFs.
26586776	7	22	theme	enhanced	1025:1032	arg1	capability					1061:1070	enhanced osteogenic differentiation capability	1025:1070	enhanced osteogenic differentiation capability	1025:1070	First, hGFs were reprogrammed into hiPSCs, which have enhanced osteogenic differentiation capability.
26586776	20	23	theme	innovative	2795:2804	arg1	approach					2806:2813	a potential innovative approach	2783:2813	a potential innovative approach for bone tissue engineering, especially large bone regeneration	2783:2877	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	20	23	theme	innovative	2795:2804	arg1	technique					2758:2766	This technique	2753:2766	This technique	2753:2766	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	5	24	with	differentiation	635:649	arg1	hiPSCs					656:661	hiPSCs	656:661	hiPSCs	656:661	However, its regulation on osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs) is unknown.
26586776	0	25	from	Fibroblasts	67:77	arg1	Cells					47:51	Patient-Derived Human Induced Pluripotent Stem Cells	0:51	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts	0:77	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	20	26	theme	bone	2861:2864	arg1	regeneration					2866:2877	especially large bone regeneration	2844:2877	especially large bone regeneration	2844:2877	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	20	26	theme	bone	2861:2864	arg1	engineering					2831:2841	bone tissue engineering	2819:2841	bone tissue engineering	2819:2841	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	19	27	theme	important	2642:2650	arg1	role					2652:2655	the important role	2638:2655	the important role of different nHA ratios in HCG scaffolds	2638:2696	The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs.
26586776	11	28	dep	increased	1407:1415	arg1	proved					1471:1476	proved	1471:1476	increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction	1407:1528	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	11	29	theme	chain	1515:1519	arg1	reaction					1521:1528	quantitative real-time polymerase chain reaction	1481:1528	quantitative real-time polymerase chain reaction	1481:1528	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	14	30	with	approach	2027:2034	arg1	gingiva					2082:2088	clinically abundant gingiva	2062:2088	clinically abundant gingiva	2062:2088	Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
26586776	14	30	with	approach	2027:2034	arg1	tissues					2046:2052	soft tissues	2041:2052	soft tissues such as clinically abundant gingiva	2041:2088	Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
26586776	7	31	contain	have	1020:1023	arg2	capability					1061:1070	enhanced osteogenic differentiation capability	1025:1070	enhanced osteogenic differentiation capability	1025:1070	First, hGFs were reprogrammed into hiPSCs, which have enhanced osteogenic differentiation capability.
26586776	7	31	contain	have	1020:1023	arg1	hiPSCs					1006:1011	hiPSCs	1006:1011	hiPSCs	1006:1011	First, hGFs were reprogrammed into hiPSCs, which have enhanced osteogenic differentiation capability.
26586776	17	32	attach	derived	2379:2385	arg2	hiPSCs					2372:2377	hiPSCs	2372:2377	hiPSCs derived from hGFs	2372:2395	hiPSCs derived from hGFs had better osteogenesis capability than that of hGFs.
26586776	17	32	attach	derived	2379:2385	arg1	hGFs					2392:2395	hGFs	2392:2395	hGFs	2392:2395	hiPSCs derived from hGFs had better osteogenesis capability than that of hGFs.
26586776	11	33	theme	real-time	1494:1502	arg1	reaction					1521:1528	quantitative real-time polymerase chain reaction	1481:1528	quantitative real-time polymerase chain reaction	1481:1528	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	19	34	theme	nHA	2670:2672	arg1	ratios					2674:2679	different nHA ratios	2660:2679	different nHA ratios	2660:2679	The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs.
26586776	20	35	theme	potential	2785:2793	arg1	approach					2806:2813	a potential innovative approach	2783:2813	a potential innovative approach for bone tissue engineering, especially large bone regeneration	2783:2877	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	20	35	theme	potential	2785:2793	arg1	technique					2758:2766	This technique	2753:2766	This technique	2753:2766	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	0	36	dep	Graft	174:178	arg1	Substitutes					180:190	Substitutes	180:190	Substitutes	180:190	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	10	37	theme	osteogenic	1266:1275	arg1	effects					1293:1299	The osteogenic differentiation effects	1262:1299	The osteogenic differentiation effects of two types of HCG scaffolds on hiPSCs	1262:1339	The osteogenic differentiation effects of two types of HCG scaffolds on hiPSCs were assessed for up to 12 weeks.
26586776	1	38	theme	embryonic	210:218	arg1	cells					246:250	adult stem cells	235:250	adult stem cells	235:250	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	38	theme	embryonic	210:218	arg1	source					278:283	the cell source	269:283	the cell source for bone tissue engineering	269:311	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	38	theme	embryonic	210:218	arg1	cells					225:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	4	39	theme	important	522:530	arg1	Nanohydroxyapatite					491:508	Nanohydroxyapatite	491:508	Nanohydroxyapatite (nHA)	491:514	Nanohydroxyapatite (nHA) is an important component of natural bone and bone tissue engineering scaffolds.
26586776	4	39	theme	important	522:530	arg1	component					532:540	an important component	519:540	an important component of natural bone and bone tissue engineering scaffolds	519:594	Nanohydroxyapatite (nHA) is an important component of natural bone and bone tissue engineering scaffolds.
26586776	0	40	theme	Nanohydroxyapatite/Chitosan/Gelatin	103:137	arg1	Scaffolds					146:154	Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds	95:154	Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes	95:190	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	5	41	theme	osteogenic	624:633	arg1	differentiation					635:649	osteogenic differentiation	624:649	osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs)	624:700	However, its regulation on osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs) is unknown.
26586776	15	42	theme	present	2108:2114	arg1	study					2116:2120	The present study	2104:2120	The present study	2104:2120	SIGNIFICANCE The present study focused on patient-personalized bone tissue engineering.
26586776	11	43	theme	hiPSCs	1455:1460	arg1	expression					1441:1450	osteogenic-related gene expression	1417:1450	osteogenic-related gene expression of hiPSCs	1417:1460	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	6	44	from	hGFs	828:831	arg1	hiPSCs					800:805	hiPSCs	800:805	hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA)	800:968	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	6	44	from	hGFs	828:831	arg1	differentiation					781:795	the osteogenic differentiation	766:795	the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA)	766:968	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	2	45	dep	ethical	364:370	arg1	concerns					372:379	concerns	372:379	concerns	372:379	However, their limitations are obvious, including ethical concerns and/or a short lifespan.
26586776	0	46	theme	Patient-Derived	0:14	arg1	Cells					47:51	Patient-Derived Human Induced Pluripotent Stem Cells	0:51	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts	0:77	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	14	47	theme	engineering	2015:2025	arg1	approach					2027:2034	an innovative autologous cell-based bone tissue engineering approach	1967:2034	an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva	1967:2088	Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
26586776	4	48	theme	bone	562:565	arg1	scaffolds					586:594	natural bone and bone tissue engineering scaffolds	545:594	natural bone and bone tissue engineering scaffolds	545:594	Nanohydroxyapatite (nHA) is an important component of natural bone and bone tissue engineering scaffolds.
26586776	10	49	theme	scaffolds	1321:1329	arg1	types					1308:1312	two types	1304:1312	two types of HCG scaffolds	1304:1329	The osteogenic differentiation effects of two types of HCG scaffolds on hiPSCs were assessed for up to 12 weeks.
26586776	0	50	theme	Induced	22:28	arg1	Cells					47:51	Patient-Derived Human Induced Pluripotent Stem Cells	0:51	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts	0:77	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	14	51	theme	bone	2003:2006	arg1	approach					2027:2034	an innovative autologous cell-based bone tissue engineering approach	1967:2034	an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva	1967:2088	Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
26586776	15	52	theme	bone	2154:2157	arg1	engineering					2166:2176	patient-personalized bone tissue engineering	2133:2176	patient-personalized bone tissue engineering	2133:2176	SIGNIFICANCE The present study focused on patient-personalized bone tissue engineering.
26586776	3	53	theme	stem	443:446	arg1	hiPSCs					455:460	hiPSCs	455:460	hiPSCs	455:460	The use of human induced pluripotent stem cells (hiPSCs) could avoid these problems.
26586776	3	53	theme	stem	443:446	arg1	cells					448:452	human induced pluripotent stem cells	417:452	human induced pluripotent stem cells (hiPSCs)	417:461	The use of human induced pluripotent stem cells (hiPSCs) could avoid these problems.
26586776	4	54	theme	engineering	574:584	arg1	scaffolds					586:594	natural bone and bone tissue engineering scaffolds	545:594	natural bone and bone tissue engineering scaffolds	545:594	Nanohydroxyapatite (nHA) is an important component of natural bone and bone tissue engineering scaffolds.
26586776	16	55	theme	nanohydroxyapatite/chitosan/gelatin	2319:2353	arg1	scaffolds					2361:2369	defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds	2311:2369	defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds	2311:2369	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	0	56	theme	Stem	42:45	arg1	Cells					47:51	Patient-Derived Human Induced Pluripotent Stem Cells	0:51	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts	0:77	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	19	57	attach	derived	2734:2740	arg1	hGFs					2747:2750	hGFs	2747:2750	hGFs	2747:2750	The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs.
26586776	19	57	attach	derived	2734:2740	arg2	hiPSCs					2727:2732	hiPSCs	2727:2732	hiPSCs derived from hGFs	2727:2750	The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs.
26586776	6	58	theme	different	888:896	arg1	ratios					902:907	different nHA ratios	888:907	different nHA ratios	888:907	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	14	59	theme	autologous	1981:1990	arg1	approach					2027:2034	an innovative autologous cell-based bone tissue engineering approach	1967:2034	an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva	1967:2088	Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
26586776	19	60	theme	osteogenesis	2701:2712	arg1	induction					2714:2722	osteogenesis induction	2701:2722	osteogenesis induction of hiPSCs derived from hGFs	2701:2750	The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs.
26586776	3	61	theme	induced	423:429	arg1	hiPSCs					455:460	hiPSCs	455:460	hiPSCs	455:460	The use of human induced pluripotent stem cells (hiPSCs) could avoid these problems.
26586776	3	61	theme	induced	423:429	arg1	cells					448:452	human induced pluripotent stem cells	417:452	human induced pluripotent stem cells (hiPSCs)	417:461	The use of human induced pluripotent stem cells (hiPSCs) could avoid these problems.
26586776	11	62	theme	immunohistochemistry	1696:1715	arg1	staining					1717:1724	OCN immunohistochemistry staining	1692:1724	OCN immunohistochemistry staining	1692:1724	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	11	63	theme	computed	1620:1627	arg1	tomography					1629:1638	cone-beam computed tomography	1610:1638	cone-beam computed tomography imaging	1610:1646	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	8	64	theme	HCG-111	1081:1087	arg1	scaffolds					1101:1109	HCG-111 and HCG-311 scaffolds	1081:1109	HCG-111 and HCG-311 scaffolds	1081:1109	Second, HCG-111 and HCG-311 scaffolds were successfully synthesized.
26586776	15	65	theme	tissue	2159:2164	arg1	engineering					2166:2176	patient-personalized bone tissue engineering	2133:2176	patient-personalized bone tissue engineering	2133:2176	SIGNIFICANCE The present study focused on patient-personalized bone tissue engineering.
26586776	4	66	theme	bone	553:556	arg1	scaffolds					586:594	natural bone and bone tissue engineering scaffolds	545:594	natural bone and bone tissue engineering scaffolds	545:594	Nanohydroxyapatite (nHA) is an important component of natural bone and bone tissue engineering scaffolds.
26586776	18	67	from	hGFs	2513:2516	arg1	differentiation					2482:2496	osteogenic differentiation	2471:2496	osteogenic differentiation of hiPSCs from hGFs	2471:2516	More interestingly, osteogenic differentiation of hiPSCs from hGFs was elevated significantly when composited with HCG-311 scaffolds in vitro and in vivo.
26586776	18	67	from	hGFs	2513:2516	arg1	hiPSCs					2501:2506	hiPSCs	2501:2506	hiPSCs from hGFs	2501:2516	More interestingly, osteogenic differentiation of hiPSCs from hGFs was elevated significantly when composited with HCG-311 scaffolds in vitro and in vivo.
26586776	8	68	theme	HCG-311	1093:1099	arg1	scaffolds					1101:1109	HCG-111 and HCG-311 scaffolds	1081:1109	HCG-111 and HCG-311 scaffolds	1081:1109	Second, HCG-111 and HCG-311 scaffolds were successfully synthesized.
26586776	6	69	theme	study	741:745	arg1	purpose					718:724	The purpose	714:724	The purpose of the present study	714:745	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	1	70	theme	UNLABELLED	193:202	arg1	cells					246:250	adult stem cells	235:250	adult stem cells	235:250	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	70	theme	UNLABELLED	193:202	arg1	source					278:283	the cell source	269:283	the cell source for bone tissue engineering	269:311	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	70	theme	UNLABELLED	193:202	arg1	cells					225:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	17	71	theme	better	2401:2406	arg1	capability					2421:2430	better osteogenesis capability	2401:2430	better osteogenesis capability	2401:2430	hiPSCs derived from hGFs had better osteogenesis capability than that of hGFs.
26586776	11	72	theme	H&E	1649:1651	arg1	staining					1653:1660	H&E staining	1649:1660	H&E staining	1649:1660	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	1	73	theme	cell	273:276	arg1	cells					246:250	adult stem cells	235:250	adult stem cells	235:250	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	73	theme	cell	273:276	arg1	source					278:283	the cell source	269:283	the cell source for bone tissue engineering	269:311	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	73	theme	cell	273:276	arg1	cells					225:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	6	74	theme	%	935:935	arg1	nHA					937:939	1 wt/vol% nHA	927:939	1 wt/vol% nHA	927:939	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	6	74	theme	%	935:935	arg1	HCG-111					918:924	HCG-111	918:924	HCG-111 (1 wt/vol% nHA)	918:940	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	5	75	theme	gingival	674:681	arg1	fibroblasts					683:693	human gingival fibroblasts	668:693	human gingival fibroblasts (hGFs)	668:700	However, its regulation on osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs) is unknown.
26586776	5	75	theme	gingival	674:681	arg1	hGFs					696:699	hGFs	696:699	hGFs	696:699	However, its regulation on osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs) is unknown.
26586776	6	76	theme	present	733:739	arg1	study					741:745	the present study	729:745	the present study	729:745	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	2	77	theme	short	390:394	arg1	lifespan					396:403	a short lifespan	388:403	a short lifespan	388:403	However, their limitations are obvious, including ethical concerns and/or a short lifespan.
26586776	13	78	theme	osteogenic	1895:1904	arg1	expression					1913:1922	osteogenic marker expression	1895:1922	osteogenic marker expression of hiPSCs from hGFs	1895:1942	The mechanism might be that the nHA addition stimulates osteogenic marker expression of hiPSCs from hGFs.
26586776	16	79	theme	derived	2265:2271	arg1	hGFs					2301:2304	hGFs	2301:2304	hGFs	2301:2304	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	16	79	theme	derived	2265:2271	arg1	fibroblasts					2288:2298	human gingival fibroblasts	2273:2298	clinically easily derived human gingival fibroblasts (hGFs)	2247:2305	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	20	80	theme	large	2855:2859	arg1	regeneration					2866:2877	especially large bone regeneration	2844:2877	especially large bone regeneration	2844:2877	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	20	80	theme	large	2855:2859	arg1	engineering					2831:2841	bone tissue engineering	2819:2841	bone tissue engineering	2819:2841	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	11	81	theme	bone-like	1571:1579	arg1	tissue					1581:1586	much bone-like tissue	1566:1586	much bone-like tissue	1566:1586	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	1	82	theme	tissue	294:299	arg1	engineering					301:311	bone tissue engineering	289:311	bone tissue engineering	289:311	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	6	83	theme	osteogenic	770:779	arg1	differentiation					781:795	the osteogenic differentiation	766:795	the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA)	766:968	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	12	84	theme	hiPSCs	1797:1802	arg1	differentiation					1778:1792	osteogenic differentiation	1767:1792	osteogenic differentiation of hiPSCs from hGFs	1767:1812	In conclusion, our study has shown that osteogenic differentiation of hiPSCs from hGFs was improved by HCG-311.
26586776	5	85	from	regulation	610:619	arg1	differentiation					635:649	osteogenic differentiation	624:649	osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs)	624:700	However, its regulation on osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs) is unknown.
26586776	16	86	theme	gingival	2279:2286	arg1	hGFs					2301:2304	hGFs	2301:2304	hGFs	2301:2304	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	16	86	theme	gingival	2279:2286	arg1	fibroblasts					2288:2298	human gingival fibroblasts	2273:2298	clinically easily derived human gingival fibroblasts (hGFs)	2247:2305	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	16	87	link	derived	2265:2271	arg1	hGFs					2301:2304	hGFs	2301:2304	hGFs	2301:2304	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	16	87	link	derived	2265:2271	arg1	fibroblasts					2288:2298	human gingival fibroblasts	2273:2298	clinically easily derived human gingival fibroblasts (hGFs)	2247:2305	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	11	88	theme	hiPSC/HCG-311	1535:1547	arg1	complexes					1549:1557	hiPSC/HCG-311 complexes	1535:1557	hiPSC/HCG-311 complexes	1535:1557	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	1	89	theme	stem	220:223	arg1	cells					246:250	adult stem cells	235:250	adult stem cells	235:250	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	89	theme	stem	220:223	arg1	source					278:283	the cell source	269:283	the cell source for bone tissue engineering	269:311	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	89	theme	stem	220:223	arg1	cells					225:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	16	90	theme	induced	2185:2191	arg1	hiPSCs					2217:2222	hiPSCs	2217:2222	hiPSCs	2217:2222	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	16	90	theme	induced	2185:2191	arg1	cells					2210:2214	Human induced pluripotent stem cells	2179:2214	Human induced pluripotent stem cells (hiPSCs)	2179:2223	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	20	91	theme	tissue	2824:2829	arg1	regeneration					2866:2877	especially large bone regeneration	2844:2877	especially large bone regeneration	2844:2877	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	20	91	theme	tissue	2824:2829	arg1	engineering					2831:2841	bone tissue engineering	2819:2841	bone tissue engineering	2819:2841	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	10	92	dep	12	1365:1366	arg1	to					1362:1363	to	1362:1363	to	1362:1363	The osteogenic differentiation effects of two types of HCG scaffolds on hiPSCs were assessed for up to 12 weeks.
26586776	6	93	theme	%	963:963	arg1	nHA					965:967	3 wt/vol% nHA	955:967	3 wt/vol% nHA	955:967	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	6	93	theme	%	963:963	arg1	HCG-311					946:952	HCG-311	946:952	HCG-311 (3 wt/vol% nHA)	946:968	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	20	94	theme	bone	2819:2822	arg1	regeneration					2866:2877	especially large bone regeneration	2844:2877	especially large bone regeneration	2844:2877	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	20	94	theme	bone	2819:2822	arg1	engineering					2831:2841	bone tissue engineering	2819:2841	bone tissue engineering	2819:2841	This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
26586776	16	95	theme	stem	2205:2208	arg1	hiPSCs					2217:2222	hiPSCs	2217:2222	hiPSCs	2217:2222	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	16	95	theme	stem	2205:2208	arg1	cells					2210:2214	Human induced pluripotent stem cells	2179:2214	Human induced pluripotent stem cells (hiPSCs)	2179:2223	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	0	96	theme	Bone	169:172	arg1	Graft					174:178	Potential Bone Graft	159:178	Potential Bone Graft Substitutes	159:190	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	1	97	theme	stem	241:244	arg1	cells					246:250	adult stem cells	235:250	adult stem cells	235:250	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	97	theme	stem	241:244	arg1	source					278:283	the cell source	269:283	the cell source for bone tissue engineering	269:311	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	97	theme	stem	241:244	arg1	cells					225:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	18	98	theme	osteogenic	2471:2480	arg1	differentiation					2482:2496	osteogenic differentiation	2471:2496	osteogenic differentiation of hiPSCs from hGFs	2471:2516	More interestingly, osteogenic differentiation of hiPSCs from hGFs was elevated significantly when composited with HCG-311 scaffolds in vitro and in vivo.
26586776	10	99	from	effects	1293:1299	arg1	hiPSCs					1334:1339	hiPSCs	1334:1339	hiPSCs	1334:1339	The osteogenic differentiation effects of two types of HCG scaffolds on hiPSCs were assessed for up to 12 weeks.
26586776	19	100	theme	present	2610:2616	arg1	study					2618:2622	The present study	2606:2622	The present study	2606:2622	The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs.
26586776	19	101	theme	different	2660:2668	arg1	ratios					2674:2679	different nHA ratios	2660:2679	different nHA ratios	2660:2679	The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs.
26586776	7	102	theme	osteogenic	1034:1043	arg1	capability					1061:1070	enhanced osteogenic differentiation capability	1025:1070	enhanced osteogenic differentiation capability	1025:1070	First, hGFs were reprogrammed into hiPSCs, which have enhanced osteogenic differentiation capability.
26586776	11	103	theme	polymerase	1504:1513	arg1	reaction					1521:1528	quantitative real-time polymerase chain reaction	1481:1528	quantitative real-time polymerase chain reaction	1481:1528	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	11	104	theme	quantitative	1481:1492	arg1	reaction					1521:1528	quantitative real-time polymerase chain reaction	1481:1528	quantitative real-time polymerase chain reaction	1481:1528	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	10	105	theme	differentiation	1277:1291	arg1	effects					1293:1299	The osteogenic differentiation effects	1262:1299	The osteogenic differentiation effects of two types of HCG scaffolds on hiPSCs	1262:1339	The osteogenic differentiation effects of two types of HCG scaffolds on hiPSCs were assessed for up to 12 weeks.
26586776	0	106	theme	Defined	95:101	arg1	Scaffolds					146:154	Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds	95:154	Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes	95:190	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	19	107	theme	ratios	2674:2679	arg1	role					2652:2655	the important role	2638:2655	the important role of different nHA ratios in HCG scaffolds	2638:2696	The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs.
26586776	1	108	theme	Human	204:208	arg1	cells					246:250	adult stem cells	235:250	adult stem cells	235:250	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	108	theme	Human	204:208	arg1	source					278:283	the cell source	269:283	the cell source for bone tissue engineering	269:311	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	1	108	theme	Human	204:208	arg1	cells					225:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells	193:229	UNLABELLED Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering.
26586776	0	109	theme	Potential	159:167	arg1	Graft					174:178	Potential Bone Graft	159:178	Potential Bone Graft Substitutes	159:190	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	0	110	theme	Porous	139:144	arg1	Scaffolds					146:154	Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds	95:154	Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes	95:190	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	15	111	theme	patient-personalized	2133:2152	arg1	engineering					2166:2176	patient-personalized bone tissue engineering	2133:2176	patient-personalized bone tissue engineering	2133:2176	SIGNIFICANCE The present study focused on patient-personalized bone tissue engineering.
26586776	13	112	from	expression	1913:1922	arg1	hGFs					1939:1942	hGFs	1939:1942	hGFs	1939:1942	The mechanism might be that the nHA addition stimulates osteogenic marker expression of hiPSCs from hGFs.
26586776	18	113	theme	HCG-311	2566:2572	arg1	scaffolds					2574:2582	HCG-311 scaffolds	2566:2582	HCG-311 scaffolds	2566:2582	More interestingly, osteogenic differentiation of hiPSCs from hGFs was elevated significantly when composited with HCG-311 scaffolds in vitro and in vivo.
26586776	10	114	theme	types	1308:1312	arg1	effects					1293:1299	The osteogenic differentiation effects	1262:1299	The osteogenic differentiation effects of two types of HCG scaffolds on hiPSCs	1262:1339	The osteogenic differentiation effects of two types of HCG scaffolds on hiPSCs were assessed for up to 12 weeks.
26586776	13	115	theme	marker	1906:1911	arg1	expression					1913:1922	osteogenic marker expression	1895:1922	osteogenic marker expression of hiPSCs from hGFs	1895:1942	The mechanism might be that the nHA addition stimulates osteogenic marker expression of hiPSCs from hGFs.
26586776	11	116	theme	gene	1436:1439	arg1	expression					1441:1450	osteogenic-related gene expression	1417:1450	osteogenic-related gene expression of hiPSCs	1417:1460	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	0	117	theme	Human	16:20	arg1	Cells					47:51	Patient-Derived Human Induced Pluripotent Stem Cells	0:51	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts	0:77	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	19	118	theme	HCG	2684:2686	arg1	scaffolds					2688:2696	HCG scaffolds	2684:2696	HCG scaffolds	2684:2696	The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs.
26586776	13	119	from	hGFs	1939:1942	arg1	expression					1913:1922	osteogenic marker expression	1895:1922	osteogenic marker expression of hiPSCs from hGFs	1895:1942	The mechanism might be that the nHA addition stimulates osteogenic marker expression of hiPSCs from hGFs.
26586776	13	119	from	hGFs	1939:1942	arg1	hiPSCs					1927:1932	hiPSCs	1927:1932	hiPSCs from hGFs	1927:1942	The mechanism might be that the nHA addition stimulates osteogenic marker expression of hiPSCs from hGFs.
26586776	12	120	from	differentiation	1778:1792	arg1	hGFs					1809:1812	hGFs	1809:1812	hGFs	1809:1812	In conclusion, our study has shown that osteogenic differentiation of hiPSCs from hGFs was improved by HCG-311.
26586776	3	121	theme	human	417:421	arg1	hiPSCs					455:460	hiPSCs	455:460	hiPSCs	455:460	The use of human induced pluripotent stem cells (hiPSCs) could avoid these problems.
26586776	3	121	theme	human	417:421	arg1	cells					448:452	human induced pluripotent stem cells	417:452	human induced pluripotent stem cells (hiPSCs)	417:461	The use of human induced pluripotent stem cells (hiPSCs) could avoid these problems.
26586776	4	122	theme	tissue	567:572	arg1	scaffolds					586:594	natural bone and bone tissue engineering scaffolds	545:594	natural bone and bone tissue engineering scaffolds	545:594	Nanohydroxyapatite (nHA) is an important component of natural bone and bone tissue engineering scaffolds.
26586776	16	123	theme	defined	2311:2317	arg1	scaffolds					2361:2369	defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds	2311:2369	defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds	2311:2369	Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds.
26586776	0	124	theme	Pluripotent	30:40	arg1	Cells					47:51	Patient-Derived Human Induced Pluripotent Stem Cells	0:51	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts	0:77	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	14	125	theme	tissue	2008:2013	arg1	approach					2027:2034	an innovative autologous cell-based bone tissue engineering approach	1967:2034	an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva	1967:2088	Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
26586776	15	126	dep	study	2116:2120	arg1	SIGNIFICANCE					2091:2102	SIGNIFICANCE	2091:2102	SIGNIFICANCE	2091:2102	SIGNIFICANCE The present study focused on patient-personalized bone tissue engineering.
26586776	4	127	theme	scaffolds	586:594	arg1	Nanohydroxyapatite					491:508	Nanohydroxyapatite	491:508	Nanohydroxyapatite (nHA)	491:514	Nanohydroxyapatite (nHA) is an important component of natural bone and bone tissue engineering scaffolds.
26586776	4	127	theme	scaffolds	586:594	arg1	component					532:540	an important component	519:540	an important component of natural bone and bone tissue engineering scaffolds	519:594	Nanohydroxyapatite (nHA) is an important component of natural bone and bone tissue engineering scaffolds.
26586776	14	128	theme	soft	2041:2044	arg1	gingiva					2082:2088	clinically abundant gingiva	2062:2088	clinically abundant gingiva	2062:2088	Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
26586776	14	128	theme	soft	2041:2044	arg1	tissues					2046:2052	soft tissues	2041:2052	soft tissues such as clinically abundant gingiva	2041:2088	Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
26586776	10	129	theme	HCG	1317:1319	arg1	scaffolds					1321:1329	HCG scaffolds	1317:1329	HCG scaffolds	1317:1329	The osteogenic differentiation effects of two types of HCG scaffolds on hiPSCs were assessed for up to 12 weeks.
26586776	13	130	theme	nHA	1871:1873	arg1	addition					1875:1882	the nHA addition	1867:1882	the nHA addition	1867:1882	The mechanism might be that the nHA addition stimulates osteogenic marker expression of hiPSCs from hGFs.
26586776	6	131	theme	nHA	898:900	arg1	ratios					902:907	different nHA ratios	888:907	different nHA ratios	888:907	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	14	132	theme	cell-based	1992:2001	arg1	approach					2027:2034	an innovative autologous cell-based bone tissue engineering approach	1967:2034	an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva	1967:2088	Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
26586776	6	133	with	scaffolds	873:881	arg1	ratios					902:907	different nHA ratios	888:907	different nHA ratios	888:907	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	3	134	theme	cells	448:452	arg1	use					410:412	The use	406:412	The use of human induced pluripotent stem cells (hiPSCs)	406:461	The use of human induced pluripotent stem cells (hiPSCs) could avoid these problems.
26586776	4	135	theme	natural	545:551	arg1	scaffolds					586:594	natural bone and bone tissue engineering scaffolds	545:594	natural bone and bone tissue engineering scaffolds	545:594	Nanohydroxyapatite (nHA) is an important component of natural bone and bone tissue engineering scaffolds.
26586776	11	136	theme	OCN	1692:1694	arg1	staining					1717:1724	OCN immunohistochemistry staining	1692:1724	OCN immunohistochemistry staining	1692:1724	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	11	137	theme	cone-beam	1610:1618	arg1	tomography					1629:1638	cone-beam computed tomography	1610:1638	cone-beam computed tomography imaging	1610:1646	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	0	138	theme	Gingival	58:65	arg1	Fibroblasts					67:77	Gingival Fibroblasts	58:77	Gingival Fibroblasts	58:77	Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
26586776	14	139	theme	innovative	1970:1979	arg1	approach					2027:2034	an innovative autologous cell-based bone tissue engineering approach	1967:2034	an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva	1967:2088	Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
26586776	3	140	theme	pluripotent	431:441	arg1	hiPSCs					455:460	hiPSCs	455:460	hiPSCs	455:460	The use of human induced pluripotent stem cells (hiPSCs) could avoid these problems.
26586776	3	140	theme	pluripotent	431:441	arg1	cells					448:452	human induced pluripotent stem cells	417:452	human induced pluripotent stem cells (hiPSCs)	417:461	The use of human induced pluripotent stem cells (hiPSCs) could avoid these problems.
26586776	14	141	theme	abundant	2073:2080	arg1	gingiva					2082:2088	clinically abundant gingiva	2062:2088	clinically abundant gingiva	2062:2088	Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
26586776	17	142	contain	had	2397:2399	arg2	capability					2421:2430	better osteogenesis capability	2401:2430	better osteogenesis capability	2401:2430	hiPSCs derived from hGFs had better osteogenesis capability than that of hGFs.
26586776	17	142	contain	had	2397:2399	arg1	hiPSCs					2372:2377	hiPSCs	2372:2377	hiPSCs derived from hGFs	2372:2395	hiPSCs derived from hGFs had better osteogenesis capability than that of hGFs.
26586776	5	143	from	fibroblasts	683:693	arg1	differentiation					635:649	osteogenic differentiation	624:649	osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs)	624:700	However, its regulation on osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs) is unknown.
26586776	6	144	from	differentiation	781:795	arg1	hGFs					828:831	patient-derived hGFs	812:831	patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA)	812:968	The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA).
26586776	9	145	theme	hiPSC/HCG	1151:1159	arg1	complexes					1161:1169	hiPSC/HCG complexes	1151:1169	hiPSC/HCG complexes	1151:1169	Finally, hiPSC/HCG complexes were cultured in vitro or subcutaneously transplanted into immunocompromised mice in vivo.
26586776	17	146	theme	osteogenesis	2408:2419	arg1	capability					2421:2430	better osteogenesis capability	2401:2430	better osteogenesis capability	2401:2430	hiPSCs derived from hGFs had better osteogenesis capability than that of hGFs.
26586776	11	147	theme	Masson	1663:1668	arg1	staining					1670:1677	Masson staining	1663:1677	Masson staining	1663:1677	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	11	148	theme	tomography	1629:1638	arg1	imaging					1640:1646	cone-beam computed tomography imaging	1610:1646	cone-beam computed tomography imaging	1610:1646	The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining.
26586776	18	149	from	differentiation	2482:2496	arg1	hGFs					2513:2516	hGFs	2513:2516	hGFs	2513:2516	More interestingly, osteogenic differentiation of hiPSCs from hGFs was elevated significantly when composited with HCG-311 scaffolds in vitro and in vivo.
24709012	0	0	theme	edible	93:98	arg1	films					100:104	gelatin-chitosan composite edible films	66:104	gelatin-chitosan composite edible films	66:104	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films.
24709012	6	1	theme	composite	852:860	arg1	films					862:866	composite films	852:866	composite films	852:866	The morphology study of gelatin, chitosan and composite films showed a compact and homogenous structure.
24709012	0	2	theme	composite	83:91	arg1	films					100:104	gelatin-chitosan composite edible films	66:104	gelatin-chitosan composite edible films	66:104	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films.
24709012	7	3	theme	β-carotene	1006:1015	arg1	bleaching					1017:1025	β-carotene bleaching	1006:1025	β-carotene bleaching	1006:1025	In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
24709012	1	4	theme	composite	232:240	arg1	films					242:246	composite films	232:246	composite films (G75/C25, G50/C50, G25/C75)	232:274	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	7	5	theme	Gram-negative	1181:1193	arg1	bacteria					1195:1202	Gram-negative bacteria	1181:1202	Gram-negative bacteria	1181:1202	In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
24709012	6	6	theme	chitosan	839:846	arg1	study					821:825	The morphology study	806:825	The morphology study of gelatin, chitosan and composite films	806:866	The morphology study of gelatin, chitosan and composite films showed a compact and homogenous structure.
24709012	7	7	theme	power	1065:1069	arg1	activities					1071:1080	reducing power activities	1056:1080	reducing power activities	1056:1080	In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
24709012	0	8	theme	films	100:104	arg1	properties					52:61	Physical, structural, antioxidant and antimicrobial properties	0:61	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films	0:104	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films.
24709012	5	9	theme	films	799:803	arg1	temperature					760:770	the transition temperature	745:770	the transition temperature (Tg)	745:775	DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films.
24709012	5	9	theme	films	799:803	arg1	ΔHg					791:793	ΔHg	791:793	ΔHg	791:793	DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films.
24709012	5	9	theme	films	799:803	arg1	Tg					773:774	Tg	773:774	Tg	773:774	DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films.
24709012	5	9	theme	films	799:803	arg1	enthalpy					781:788	enthalpy	781:788	enthalpy (ΔHg) of films	781:803	DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films.
24709012	5	10	theme	content	704:710	arg1	increase					683:690	the increase	679:690	the increase of chitosan content in the composite films	679:733	DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films.
24709012	3	11	theme	Composite	456:464	arg1	films					466:470	Composite films	456:470	Composite films	456:470	Composite films show no significant difference in tensile strength (TS), thickness and transparency.
24709012	3	12	from	difference	492:501	arg1	strength					514:521	tensile strength	506:521	tensile strength (TS)	506:526	Composite films show no significant difference in tensile strength (TS), thickness and transparency.
24709012	3	12	from	difference	492:501	arg1	transparency					543:554	transparency	543:554	transparency	543:554	Composite films show no significant difference in tensile strength (TS), thickness and transparency.
24709012	3	12	from	difference	492:501	arg1	TS					524:525	TS	524:525	TS	524:525	Composite films show no significant difference in tensile strength (TS), thickness and transparency.
24709012	3	12	from	difference	492:501	arg1	thickness					529:537	thickness	529:537	thickness	529:537	Composite films show no significant difference in tensile strength (TS), thickness and transparency.
24709012	2	13	theme	higher	366:371	arg1	strength					381:388	higher tensile strength	366:388	higher tensile strength	366:388	The results indicated that chitosan film had higher tensile strength and lower elongation at break when compared with the other films.
24709012	7	14	theme	reducing	1056:1063	arg1	activities					1071:1080	reducing power activities	1056:1080	reducing power activities	1056:1080	In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
24709012	3	15	theme	tensile	506:512	arg1	strength					514:521	tensile strength	506:521	tensile strength (TS)	506:526	Composite films show no significant difference in tensile strength (TS), thickness and transparency.
24709012	3	15	theme	tensile	506:512	arg1	TS					524:525	TS	524:525	TS	524:525	Composite films show no significant difference in tensile strength (TS), thickness and transparency.
24709012	4	16	theme	total	616:620	arg1	miscibility					622:632	total miscibility	616:632	total miscibility between both polymers	616:654	The structural properties evaluated by FTIR and DSC showed total miscibility between both polymers.
24709012	7	17	theme	DPPH	1028:1031	arg1	radical-scavenging					1033:1050	DPPH radical-scavenging	1028:1050	DPPH radical-scavenging	1028:1050	In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
24709012	6	18	theme	gelatin	830:836	arg1	study					821:825	The morphology study	806:825	The morphology study of gelatin, chitosan and composite films	806:866	The morphology study of gelatin, chitosan and composite films showed a compact and homogenous structure.
24709012	0	19	theme	Physical	0:7	arg1	properties					52:61	Physical, structural, antioxidant and antimicrobial properties	0:61	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films	0:104	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films.
24709012	4	20	theme	structural	561:570	arg1	properties					572:581	The structural properties	557:581	The structural properties evaluated by FTIR and DSC	557:607	The structural properties evaluated by FTIR and DSC showed total miscibility between both polymers.
24709012	1	21	from	films	242:246	arg1	Physico-chemical					107:122	Physico-chemical	107:122	Physico-chemical	107:122	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	2	22	theme	lower	394:398	arg1	elongation					400:409	lower elongation	394:409	lower elongation	394:409	The results indicated that chitosan film had higher tensile strength and lower elongation at break when compared with the other films.
24709012	0	23	theme	structural	10:19	arg1	properties					52:61	Physical, structural, antioxidant and antimicrobial properties	0:61	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films	0:104	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films.
24709012	7	24	theme	high	965:968	arg1	activities					982:991	a high antioxidant activities	963:991	a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities	963:1080	In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
24709012	6	25	theme	morphology	810:819	arg1	study					821:825	The morphology study	806:825	The morphology study of gelatin, chitosan and composite films	806:866	The morphology study of gelatin, chitosan and composite films showed a compact and homogenous structure.
24709012	3	26	theme	significant	480:490	arg1	difference					492:501	no significant difference	477:501	no significant difference in tensile strength (TS), thickness and transparency	477:554	Composite films show no significant difference in tensile strength (TS), thickness and transparency.
24709012	2	27	theme	other	443:447	arg1	films					449:453	the other films	439:453	the other films	439:453	The results indicated that chitosan film had higher tensile strength and lower elongation at break when compared with the other films.
24709012	6	28	theme	homogenous	889:898	arg1	structure					900:908	a compact and homogenous structure	875:908	a compact and homogenous structure	875:908	The morphology study of gelatin, chitosan and composite films showed a compact and homogenous structure.
24709012	0	29	theme	antioxidant	22:32	arg1	properties					52:61	Physical, structural, antioxidant and antimicrobial properties	0:61	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films	0:104	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films.
24709012	5	30	theme	transition	749:758	arg1	temperature					760:770	the transition temperature	745:770	the transition temperature (Tg)	745:775	DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films.
24709012	5	30	theme	transition	749:758	arg1	Tg					773:774	Tg	773:774	Tg	773:774	DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films.
24709012	2	31	contain	had	362:364	arg2	elongation					400:409	lower elongation	394:409	lower elongation	394:409	The results indicated that chitosan film had higher tensile strength and lower elongation at break when compared with the other films.
24709012	2	31	contain	had	362:364	arg1	chitosan					348:355	chitosan film	348:360	chitosan film	348:360	The results indicated that chitosan film had higher tensile strength and lower elongation at break when compared with the other films.
24709012	2	31	contain	had	362:364	arg2	strength					381:388	higher tensile strength	366:388	higher tensile strength	366:388	The results indicated that chitosan film had higher tensile strength and lower elongation at break when compared with the other films.
24709012	7	32	theme	higher	1124:1129	arg1	activity					1145:1152	higher antimicrobial activity	1124:1152	higher antimicrobial activity against Gram-positive than Gram-negative bacteria	1124:1202	In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
24709012	2	33	theme	tensile	373:379	arg1	strength					381:388	higher tensile strength	366:388	higher tensile strength	366:388	The results indicated that chitosan film had higher tensile strength and lower elongation at break when compared with the other films.
24709012	6	34	theme	compact	877:883	arg1	structure					900:908	a compact and homogenous structure	875:908	a compact and homogenous structure	875:908	The morphology study of gelatin, chitosan and composite films showed a compact and homogenous structure.
24709012	1	35	from	shrimp	200:205	arg1	Physico-chemical					107:122	Physico-chemical	107:122	Physico-chemical	107:122	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	1	36	theme	mechanical	128:137	arg1	properties					139:148	mechanical properties	128:148	mechanical properties of cuttlefish skin gelatin (G), chitosan (C)	128:193	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	0	37	theme	antimicrobial	38:50	arg1	properties					52:61	Physical, structural, antioxidant and antimicrobial properties	0:61	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films	0:104	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films.
24709012	1	38	dep	films	242:246	arg1	G25/C75					267:273	G25/C75	267:273	G25/C75	267:273	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	7	39	theme	G75/C25	936:942	arg1	films					944:948	gelatin and G75/C25 films	924:948	gelatin and G75/C25 films	924:948	In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
24709012	5	40	theme	DSC	657:659	arg1	scans					661:665	DSC scans	657:665	DSC scans	657:665	DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films.
24709012	7	41	theme	antioxidant	970:980	arg1	activities					982:991	a high antioxidant activities	963:991	a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities	963:1080	In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
24709012	5	42	from	increase	683:690	arg1	films					729:733	the composite films	715:733	the composite films	715:733	DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films.
24709012	7	43	theme	gelatin	924:930	arg1	films					944:948	gelatin and G75/C25 films	924:948	gelatin and G75/C25 films	924:948	In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
24709012	1	44	theme	cuttlefish	153:162	arg1	chitosan					182:189	chitosan	182:189	chitosan (C)	182:193	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	1	44	theme	cuttlefish	153:162	arg1	gelatin					169:175	cuttlefish skin gelatin	153:175	cuttlefish skin gelatin (G)	153:179	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	1	44	theme	cuttlefish	153:162	arg1	G					178:178	G	178:178	G	178:178	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	1	45	theme	Penaeus	208:214	arg1	kerathurus					216:225	Penaeus kerathurus	208:225	Penaeus kerathurus	208:225	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	1	45	theme	Penaeus	208:214	arg1	shrimp					200:205	shrimp	200:205	shrimp (Penaeus kerathurus)	200:226	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	0	46	theme	gelatin-chitosan	66:81	arg1	films					100:104	gelatin-chitosan composite edible films	66:104	gelatin-chitosan composite edible films	66:104	Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films.
24709012	1	47	theme	skin	164:167	arg1	chitosan					182:189	chitosan	182:189	chitosan (C)	182:193	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	1	47	theme	skin	164:167	arg1	gelatin					169:175	cuttlefish skin gelatin	153:175	cuttlefish skin gelatin (G)	153:179	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	1	47	theme	skin	164:167	arg1	G					178:178	G	178:178	G	178:178	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	5	48	theme	composite	719:727	arg1	films					729:733	the composite films	715:733	the composite films	715:733	DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films.
24709012	6	49	theme	films	862:866	arg1	study					821:825	The morphology study	806:825	The morphology study of gelatin, chitosan and composite films	806:866	The morphology study of gelatin, chitosan and composite films showed a compact and homogenous structure.
24709012	7	50	theme	antimicrobial	1131:1143	arg1	activity					1145:1152	higher antimicrobial activity	1124:1152	higher antimicrobial activity against Gram-positive than Gram-negative bacteria	1124:1202	In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
24709012	1	51	theme	gelatin	169:175	arg1	properties					139:148	mechanical properties	128:148	mechanical properties of cuttlefish skin gelatin (G), chitosan (C)	128:193	Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated.
24709012	5	52	theme	chitosan	695:702	arg1	content					704:710	chitosan content	695:710	chitosan content	695:710	DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films.
24999513	6	0	theme	molecules	1067:1075	arg1	capability					1053:1062	high diffusion capability	1038:1062	high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold	1038:1155	In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
24999513	1	1	theme	microscale	153:162	arg1	spinning					170:177	microscale fiber spinning	153:177	microscale fiber spinning	153:177	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	6	2	theme	fibrous	1011:1017	arg1	scaffold					1019:1026	the proposed fibrous scaffold	998:1026	the proposed fibrous scaffold	998:1026	In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
24999513	2	3	theme	scaffolds	330:338	arg1	variety					311:317	a variety	309:317	a variety of fibrous scaffolds with diverse compositions	309:364	This platform was also used to fabricate a variety of fibrous scaffolds with diverse compositions without the use of complicated devices.
24999513	2	3	theme	scaffolds	330:338	arg1	scaffolds					330:338	fibrous scaffolds	322:338	fibrous scaffolds with diverse compositions	322:364	This platform was also used to fabricate a variety of fibrous scaffolds with diverse compositions without the use of complicated devices.
24999513	1	4	theme	single	238:243	arg1	platform					258:265	a single microfluidic platform	236:265	a single microfluidic platform	236:265	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	0	5	theme	microfluidic	97:108	arg1	platform					110:117	a single microfluidic platform	88:117	a single microfluidic platform	88:117	One-stop microfiber spinning and fabrication of a fibrous cell-encapsulated scaffold on a single microfluidic platform.
24999513	3	6	theme	primary	550:556	arg1	hepatocytes					558:568	primary hepatocytes	550:568	primary hepatocytes	550:568	We explored the potential utility of the fibrous scaffolds for tissue engineering applications by constructing a fibrous scaffold encapsulating primary hepatocytes.
24999513	6	7	theme	massive	1165:1171	arg1	culture					1178:1184	massive cell culture	1165:1184	massive cell culture	1165:1184	In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
24999513	1	8	theme	microfluidic	245:256	arg1	platform					258:265	a single microfluidic platform	236:265	a single microfluidic platform	236:265	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	0	9	theme	single	90:95	arg1	platform					110:117	a single microfluidic platform	88:117	a single microfluidic platform	88:117	One-stop microfiber spinning and fabrication of a fibrous cell-encapsulated scaffold on a single microfluidic platform.
24999513	6	10	theme	encapsulated	1112:1123	arg1	cells					1125:1129	encapsulated cells	1112:1129	encapsulated cells	1112:1129	In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
24999513	5	11	theme	encapsulated	866:877	arg1	cells					879:883	encapsulated cells	866:883	encapsulated cells	866:883	The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator.
24999513	5	12	theme	fibrous	749:755	arg1	structure					757:765	the fibrous structure	745:765	the fibrous structure used to form a scaffold	745:789	The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator.
24999513	3	13	theme	engineering	476:486	arg1	applications					488:499	tissue engineering applications	469:499	tissue engineering applications	469:499	We explored the potential utility of the fibrous scaffolds for tissue engineering applications by constructing a fibrous scaffold encapsulating primary hepatocytes.
24999513	5	14	theme	alginate	847:854	arg1	fibers					856:861	alginate fibers	847:861	alginate fibers	847:861	The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator.
24999513	4	15	theme	higher	638:643	arg1	viability					645:653	higher viability	638:653	higher viability comparing with 3D alginate non-fibrous block	638:698	The cells in scaffold were cultured over seven days and maintained higher viability comparing with 3D alginate non-fibrous block.
24999513	6	16	theme	diffusion	1043:1051	arg1	capability					1053:1062	high diffusion capability	1038:1062	high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold	1038:1155	In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
24999513	3	17	theme	potential	422:430	arg1	utility					432:438	the potential utility	418:438	the potential utility of the fibrous scaffolds for tissue engineering applications	418:499	We explored the potential utility of the fibrous scaffolds for tissue engineering applications by constructing a fibrous scaffold encapsulating primary hepatocytes.
24999513	6	18	theme	high	1038:1041	arg1	capability					1053:1062	high diffusion capability	1038:1062	high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold	1038:1155	In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
24999513	4	19	theme	3D	670:671	arg1	block					694:698	3D alginate non-fibrous block	670:698	3D alginate non-fibrous block	670:698	The cells in scaffold were cultured over seven days and maintained higher viability comparing with 3D alginate non-fibrous block.
24999513	3	20	theme	fibrous	519:525	arg1	scaffold					527:534	a fibrous scaffold	517:534	a fibrous scaffold encapsulating primary hepatocytes	517:568	We explored the potential utility of the fibrous scaffolds for tissue engineering applications by constructing a fibrous scaffold encapsulating primary hepatocytes.
24999513	4	21	from	cells	575:579	arg1	scaffold					584:591	scaffold	584:591	scaffold	584:591	The cells in scaffold were cultured over seven days and maintained higher viability comparing with 3D alginate non-fibrous block.
24999513	1	22	theme	in	187:188	arg1	construction					195:206	the in situ construction	183:206	the in situ construction of a 3D fibrous scaffold on a single microfluidic platform	183:265	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	6	23	theme	cell	1173:1176	arg1	culture					1178:1184	massive cell culture	1165:1184	massive cell culture	1165:1184	In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
24999513	3	24	theme	fibrous	447:453	arg1	scaffolds					455:463	the fibrous scaffolds	443:463	the fibrous scaffolds	443:463	We explored the potential utility of the fibrous scaffolds for tissue engineering applications by constructing a fibrous scaffold encapsulating primary hepatocytes.
24999513	6	25	theme	non-fibrous	1136:1146	arg1	scaffold					1148:1155	non-fibrous scaffold	1136:1155	non-fibrous scaffold	1136:1155	In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
24999513	1	26	from	platform	258:265	arg1	construction					195:206	the in situ construction	183:206	the in situ construction of a 3D fibrous scaffold on a single microfluidic platform	183:265	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	1	26	from	platform	258:265	arg1	spinning					170:177	microscale fiber spinning	153:177	microscale fiber spinning	153:177	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	1	27	theme	fiber	164:168	arg1	spinning					170:177	microscale fiber spinning	153:177	microscale fiber spinning	153:177	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	3	28	theme	scaffolds	455:463	arg1	utility					432:438	the potential utility	418:438	the potential utility of the fibrous scaffolds for tissue engineering applications	418:499	We explored the potential utility of the fibrous scaffolds for tissue engineering applications by constructing a fibrous scaffold encapsulating primary hepatocytes.
24999513	5	29	theme	platform	728:735	arg1	advantage					710:718	The main advantage	701:718	The main advantage of this platform	701:735	The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator.
24999513	4	30	theme	non-fibrous	682:692	arg1	block					694:698	3D alginate non-fibrous block	670:698	3D alginate non-fibrous block	670:698	The cells in scaffold were cultured over seven days and maintained higher viability comparing with 3D alginate non-fibrous block.
24999513	2	31	theme	devices	397:403	arg1	use					378:380	the use	374:380	the use of complicated devices	374:403	This platform was also used to fabricate a variety of fibrous scaffolds with diverse compositions without the use of complicated devices.
24999513	4	32	theme	alginate	673:680	arg1	block					694:698	3D alginate non-fibrous block	670:698	3D alginate non-fibrous block	670:698	The cells in scaffold were cultured over seven days and maintained higher viability comparing with 3D alginate non-fibrous block.
24999513	6	33	theme	proposed	1002:1009	arg1	scaffold					1019:1026	the proposed fibrous scaffold	998:1026	the proposed fibrous scaffold	998:1026	In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
24999513	1	34	dep	in	187:188	arg1	situ					190:193	situ	190:193	situ	190:193	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	2	35	theme	complicated	385:395	arg1	devices					397:403	complicated devices	385:403	complicated devices	385:403	This platform was also used to fabricate a variety of fibrous scaffolds with diverse compositions without the use of complicated devices.
24999513	0	36	theme	fibrous	50:56	arg1	scaffold					76:83	a fibrous cell-encapsulated scaffold	48:83	a fibrous cell-encapsulated scaffold	48:83	One-stop microfiber spinning and fabrication of a fibrous cell-encapsulated scaffold on a single microfluidic platform.
24999513	1	37	from	scaffold	224:231	arg1	platform					258:265	a single microfluidic platform	236:265	a single microfluidic platform	236:265	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	2	38	theme	fibrous	322:328	arg1	scaffolds					330:338	fibrous scaffolds	322:338	fibrous scaffolds with diverse compositions	322:364	This platform was also used to fabricate a variety of fibrous scaffolds with diverse compositions without the use of complicated devices.
24999513	5	39	theme	single	927:932	arg1	platform					934:941	a single platform	925:941	a single platform without the intervention of the operator	925:982	The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator.
24999513	5	40	theme	main	705:708	arg1	advantage					710:718	The main advantage	701:718	The main advantage of this platform	701:735	The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator.
24999513	2	41	with	scaffolds	330:338	arg1	compositions					353:364	diverse compositions	345:364	diverse compositions	345:364	This platform was also used to fabricate a variety of fibrous scaffolds with diverse compositions without the use of complicated devices.
24999513	5	42	dep	fibers	856:861	arg1	performed					912:920	performed	912:920	are performed in a single platform without the intervention of the operator	908:982	The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator.
24999513	5	42	dep	fibers	856:861	arg1	the					825:827	the	825:827	the	825:827	The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator.
24999513	1	43	from	construction	195:206	arg1	platform					258:265	a single microfluidic platform	236:265	a single microfluidic platform	236:265	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	2	44	used	used	291:294	arg2	platform					273:280	This platform	268:280	This platform	268:280	This platform was also used to fabricate a variety of fibrous scaffolds with diverse compositions without the use of complicated devices.
24999513	1	45	theme	3D	213:214	arg1	scaffold					224:231	a 3D fibrous scaffold	211:231	a 3D fibrous scaffold on a single microfluidic platform	211:265	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	5	46	theme	operator	975:982	arg1	intervention					955:966	the intervention	951:966	the intervention of the operator	951:982	The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator.
24999513	3	47	theme	tissue	469:474	arg1	applications					488:499	tissue engineering applications	469:499	tissue engineering applications	469:499	We explored the potential utility of the fibrous scaffolds for tissue engineering applications by constructing a fibrous scaffold encapsulating primary hepatocytes.
24999513	1	48	theme	fibrous	216:222	arg1	scaffold					224:231	a 3D fibrous scaffold	211:231	a 3D fibrous scaffold on a single microfluidic platform	211:265	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	0	49	theme	scaffold	76:83	arg1	spinning					20:27	spinning	20:27	spinning	20:27	One-stop microfiber spinning and fabrication of a fibrous cell-encapsulated scaffold on a single microfluidic platform.
24999513	0	49	theme	scaffold	76:83	arg1	fabrication					33:43	fabrication	33:43	fabrication	33:43	One-stop microfiber spinning and fabrication of a fibrous cell-encapsulated scaffold on a single microfluidic platform.
24999513	6	50	theme	cells	1125:1129	arg1	viability					1099:1107	better viability	1092:1107	better viability of encapsulated cells	1092:1129	In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
24999513	5	51	theme	weak	842:845	arg1	fibers					856:861	alginate fibers	847:861	alginate fibers	847:861	The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator.
24999513	6	52	theme	better	1092:1097	arg1	viability					1099:1107	better viability	1092:1107	better viability of encapsulated cells	1092:1129	In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
24999513	1	53	theme	scaffold	224:231	arg1	construction					195:206	the in situ construction	183:206	the in situ construction of a 3D fibrous scaffold on a single microfluidic platform	183:265	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	1	53	theme	scaffold	224:231	arg1	spinning					170:177	microscale fiber spinning	153:177	microscale fiber spinning	153:177	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	0	54	theme	cell-encapsulated	58:74	arg1	scaffold					76:83	a fibrous cell-encapsulated scaffold	48:83	a fibrous cell-encapsulated scaffold	48:83	One-stop microfiber spinning and fabrication of a fibrous cell-encapsulated scaffold on a single microfluidic platform.
24999513	1	55	from	spinning	170:177	arg1	platform					258:265	a single microfluidic platform	236:265	a single microfluidic platform	236:265	This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform.
24999513	2	56	theme	diverse	345:351	arg1	compositions					353:364	diverse compositions	345:364	diverse compositions	345:364	This platform was also used to fabricate a variety of fibrous scaffolds with diverse compositions without the use of complicated devices.
26017287	7	0	theme	continuous	1052:1061	arg1	production					1072:1081	a continuous filament production	1050:1081	a continuous filament production	1050:1081	The process used in this study has a good potential for upscaling providing a continuous filament production with well-controlled speed, but further work is required to increase the orientation and subsequently the mechanical properties.
26017287	5	1	theme	wt	790:791	arg1	%					793:793	6.5 wt %	786:793	6.5 wt %	786:793	A minimum concentration of 6.5 wt % was required for continuous filament spinning using the current setup.
26017287	5	2	theme	current	851:857	arg1	setup					859:863	the current setup	847:863	the current setup	847:863	A minimum concentration of 6.5 wt % was required for continuous filament spinning using the current setup.
26017287	6	3	theme	CNF	953:955	arg1	orientation					934:944	the orientation	930:944	the orientation of the CNF in the filament	930:971	However, this relatively high concentration is thought to limit the orientation of the CNF in the filament.
26017287	7	4	theme	filament	1063:1070	arg1	production					1072:1081	a continuous filament production	1050:1081	a continuous filament production	1050:1081	The process used in this study has a good potential for upscaling providing a continuous filament production with well-controlled speed, but further work is required to increase the orientation and subsequently the mechanical properties.
26017287	2	5	from	cultivation	330:340	arg1	rachis					297:302	banana rachis	290:302	banana rachis	290:302	The CNF were extracted from banana rachis, a bioresidue from banana cultivation.
26017287	2	5	from	cultivation	330:340	arg1	bioresidue					307:316	a bioresidue	305:316	a bioresidue from banana cultivation	305:340	The CNF were extracted from banana rachis, a bioresidue from banana cultivation.
26017287	0	6	from	Bioresidue	76:85	arg1	Nanofibers					60:69	Cellulose Nanofibers	50:69	Cellulose Nanofibers from Bioresidue	50:85	Dry-Spun Single-Filament Fibers Comprising Solely Cellulose Nanofibers from Bioresidue.
26017287	3	7	theme	spinning	368:375	arg1	rate					377:380	spinning rate	368:380	spinning rate	368:380	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	4	8	theme	increased	709:717	arg1	orientation					719:729	an increased orientation	706:729	an increased orientation of the CNF in the filament	706:756	This improvement is believed to be due to an increased orientation of the CNF in the filament.
26017287	6	9	theme	high	891:894	arg1	concentration					896:908	this relatively high concentration	875:908	this relatively high concentration	875:908	However, this relatively high concentration is thought to limit the orientation of the CNF in the filament.
26017287	2	10	theme	banana	290:295	arg1	rachis					297:302	banana rachis	290:302	banana rachis	290:302	The CNF were extracted from banana rachis, a bioresidue from banana cultivation.
26017287	2	10	theme	banana	290:295	arg1	bioresidue					307:316	a bioresidue	305:316	a bioresidue from banana cultivation	305:340	The CNF were extracted from banana rachis, a bioresidue from banana cultivation.
26017287	2	11	theme	banana	323:328	arg1	cultivation					330:340	banana cultivation	323:340	banana cultivation	323:340	The CNF were extracted from banana rachis, a bioresidue from banana cultivation.
26017287	5	12	theme	%	793:793	arg1	concentration					769:781	A minimum concentration	759:781	A minimum concentration of 6.5 wt %	759:793	A minimum concentration of 6.5 wt % was required for continuous filament spinning using the current setup.
26017287	3	13	dep	222	598:600	arg1	to					595:596	to	595:596	to	595:596	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	3	14	theme	filaments	439:447	arg1	concentration					387:399	CNF concentration	383:399	CNF concentration	383:399	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	3	14	theme	filaments	439:447	arg1	properties					421:430	the mechanical properties	406:430	the mechanical properties of the filaments	406:447	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	3	14	theme	filaments	439:447	arg1	rate					377:380	spinning rate	368:380	spinning rate	368:380	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	3	15	theme	mechanical	410:419	arg1	properties					421:430	the mechanical properties	406:430	the mechanical properties of the filaments	406:447	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	4	16	from	orientation	719:729	arg1	filament					749:756	the filament	745:756	the filament	745:756	This improvement is believed to be due to an increased orientation of the CNF in the filament.
26017287	4	17	theme	CNF	738:740	arg1	orientation					719:729	an increased orientation	706:729	an increased orientation of the CNF in the filament	706:756	This improvement is believed to be due to an increased orientation of the CNF in the filament.
26017287	5	18	theme	continuous	812:821	arg1	spinning					832:839	continuous filament spinning	812:839	continuous filament spinning using the current setup	812:863	A minimum concentration of 6.5 wt % was required for continuous filament spinning using the current setup.
26017287	0	19	theme	Single-Filament	9:23	arg1	Fibers					25:30	Dry-Spun Single-Filament Fibers	0:30	Dry-Spun Single-Filament Fibers	0:30	Dry-Spun Single-Filament Fibers Comprising Solely Cellulose Nanofibers from Bioresidue.
26017287	7	20	theme	good	1011:1014	arg1	potential					1016:1024	a good potential	1009:1024	a good potential for upscaling providing a continuous filament production with well-controlled speed	1009:1108	The process used in this study has a good potential for upscaling providing a continuous filament production with well-controlled speed, but further work is required to increase the orientation and subsequently the mechanical properties.
26017287	7	21	theme	further	1115:1121	arg1	work					1123:1126	further work	1115:1126	further work	1115:1126	The process used in this study has a good potential for upscaling providing a continuous filament production with well-controlled speed, but further work is required to increase the orientation and subsequently the mechanical properties.
26017287	5	22	theme	filament	823:830	arg1	spinning					832:839	continuous filament spinning	812:839	continuous filament spinning using the current setup	812:863	A minimum concentration of 6.5 wt % was required for continuous filament spinning using the current setup.
26017287	0	23	theme	Dry-Spun	0:7	arg1	Fibers					25:30	Dry-Spun Single-Filament Fibers	0:30	Dry-Spun Single-Filament Fibers	0:30	Dry-Spun Single-Filament Fibers Comprising Solely Cellulose Nanofibers from Bioresidue.
26017287	7	24	theme	well-controlled	1088:1102	arg1	speed					1104:1108	well-controlled speed	1088:1108	well-controlled speed	1088:1108	The process used in this study has a good potential for upscaling providing a continuous filament production with well-controlled speed, but further work is required to increase the orientation and subsequently the mechanical properties.
26017287	1	25	theme	low-cost	109:116	arg1	filaments					147:155	low-cost and environmentally friendly filaments	109:155	low-cost and environmentally friendly filaments of native cellulose	109:175	We demonstrated that low-cost and environmentally friendly filaments of native cellulose can be prepared by dry spinning an aqueous suspension of cellulose nanofibers (CNF).
26017287	1	26	theme	aqueous	212:218	arg1	suspension					220:229	an aqueous suspension	209:229	an aqueous suspension of cellulose nanofibers (CNF)	209:259	We demonstrated that low-cost and environmentally friendly filaments of native cellulose can be prepared by dry spinning an aqueous suspension of cellulose nanofibers (CNF).
26017287	3	27	theme	CNF	383:385	arg1	concentration					387:399	CNF concentration	383:399	CNF concentration	383:399	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	1	28	theme	environmentally	122:136	arg1	filaments					147:155	low-cost and environmentally friendly filaments	109:155	low-cost and environmentally friendly filaments of native cellulose	109:175	We demonstrated that low-cost and environmentally friendly filaments of native cellulose can be prepared by dry spinning an aqueous suspension of cellulose nanofibers (CNF).
26017287	1	29	theme	cellulose	167:175	arg1	filaments					147:155	low-cost and environmentally friendly filaments	109:155	low-cost and environmentally friendly filaments of native cellulose	109:175	We demonstrated that low-cost and environmentally friendly filaments of native cellulose can be prepared by dry spinning an aqueous suspension of cellulose nanofibers (CNF).
26017287	0	30	theme	Cellulose	50:58	arg1	Nanofibers					60:69	Cellulose Nanofibers	50:69	Cellulose Nanofibers from Bioresidue	50:85	Dry-Spun Single-Filament Fibers Comprising Solely Cellulose Nanofibers from Bioresidue.
26017287	7	31	contain	has	1005:1007	arg1	process					978:984	The process	974:984	The process used in this study	974:1003	The process used in this study has a good potential for upscaling providing a continuous filament production with well-controlled speed, but further work is required to increase the orientation and subsequently the mechanical properties.
26017287	7	31	contain	has	1005:1007	arg2	potential					1016:1024	a good potential	1009:1024	a good potential for upscaling providing a continuous filament production with well-controlled speed	1009:1108	The process used in this study has a good potential for upscaling providing a continuous filament production with well-controlled speed, but further work is required to increase the orientation and subsequently the mechanical properties.
26017287	1	32	theme	cellulose	234:242	arg1	CNF					256:258	CNF	256:258	CNF	256:258	We demonstrated that low-cost and environmentally friendly filaments of native cellulose can be prepared by dry spinning an aqueous suspension of cellulose nanofibers (CNF).
26017287	1	32	theme	cellulose	234:242	arg1	nanofibers					244:253	cellulose nanofibers	234:253	cellulose nanofibers (CNF)	234:259	We demonstrated that low-cost and environmentally friendly filaments of native cellulose can be prepared by dry spinning an aqueous suspension of cellulose nanofibers (CNF).
26017287	3	33	theme	lowest	615:620	arg1	concentration					622:634	the lowest concentration	611:634	the lowest concentration	611:634	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	1	34	theme	friendly	138:145	arg1	filaments					147:155	low-cost and environmentally friendly filaments	109:155	low-cost and environmentally friendly filaments of native cellulose	109:175	We demonstrated that low-cost and environmentally friendly filaments of native cellulose can be prepared by dry spinning an aqueous suspension of cellulose nanofibers (CNF).
26017287	3	35	used	used	658:661	arg2	speed					648:652	highest speed	640:652	highest speed	640:652	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	3	35	used	used	658:661	arg2	concentration					622:634	the lowest concentration	611:634	the lowest concentration	611:634	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	7	36	theme	mechanical	1189:1198	arg1	properties					1200:1209	the mechanical properties	1185:1209	the mechanical properties	1185:1209	The process used in this study has a good potential for upscaling providing a continuous filament production with well-controlled speed, but further work is required to increase the orientation and subsequently the mechanical properties.
26017287	6	37	from	orientation	934:944	arg1	filament					964:971	the filament	960:971	the filament	960:971	However, this relatively high concentration is thought to limit the orientation of the CNF in the filament.
26017287	1	38	theme	nanofibers	244:253	arg1	suspension					220:229	an aqueous suspension	209:229	an aqueous suspension of cellulose nanofibers (CNF)	209:259	We demonstrated that low-cost and environmentally friendly filaments of native cellulose can be prepared by dry spinning an aqueous suspension of cellulose nanofibers (CNF).
26017287	3	39	theme	highest	640:646	arg1	speed					648:652	highest speed	640:652	highest speed	640:652	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	1	40	theme	native	160:165	arg1	cellulose					167:175	native cellulose	160:175	native cellulose	160:175	We demonstrated that low-cost and environmentally friendly filaments of native cellulose can be prepared by dry spinning an aqueous suspension of cellulose nanofibers (CNF).
26017287	3	41	dep	12.6	550:553	arg1	to					547:548	to	547:548	to	547:548	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	3	42	theme	filaments	514:522	arg1	modulus					499:505	the modulus	495:505	the modulus of the filaments	495:522	The relationship between spinning rate, CNF concentration, and the mechanical properties of the filaments were investigated and the results showed that the modulus of the filaments was increased from 7.8 to 12.6 GPa and the strength increased from 131 to 222 MPa when the lowest concentration and highest speed was used.
26017287	5	43	theme	minimum	761:767	arg1	concentration					769:781	A minimum concentration	759:781	A minimum concentration of 6.5 wt %	759:793	A minimum concentration of 6.5 wt % was required for continuous filament spinning using the current setup.
28115095	6	0	theme	gravimetric	715:725	arg1	TGA					737:739	TGA	737:739	TGA	737:739	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	0	theme	gravimetric	715:725	arg1	analysis					727:734	thermo gravimetric analysis	708:734	thermo gravimetric analysis (TGA)	708:740	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	7	1	from	break	954:958	arg1	modulus					928:934	modulus	928:934	modulus	928:934	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	7	1	from	break	954:958	arg1	strength					918:925	The average tensile strength	898:925	The average tensile strength	898:925	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	7	1	from	break	954:958	arg1	111±19MPa					1012:1020	111±19MPa	1012:1020	111±19MPa	1012:1020	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	7	1	from	break	954:958	arg1	elongation					940:949	elongation	940:949	elongation	940:949	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	6	2	theme	fibers	837:842	arg1	characterization					813:828	structural characterization	802:828	structural characterization	802:828	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	8	3	theme	industrial	1158:1167	arg1	applications					1169:1180	various industrial applications	1150:1180	various industrial applications	1150:1180	The notable properties of regenerated cellulose films advocate their effectiveness for various industrial applications.
28115095	5	4	theme	cellulose	613:621	arg1	process					634:640	the cellulose extraction process	609:640	the cellulose extraction process	609:640	The results indicated that the content of the components other than cellulose was significantly decreased during the cellulose extraction process.
28115095	7	5	theme	regenerated	967:977	arg1	films					989:993	the regenerated cellulose films	963:993	the regenerated cellulose films	963:993	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	8	6	theme	various	1150:1156	arg1	applications					1169:1180	various industrial applications	1150:1180	various industrial applications	1150:1180	The notable properties of regenerated cellulose films advocate their effectiveness for various industrial applications.
28115095	4	7	theme	chemical	477:484	arg1	analysis					486:493	chemical analysis	477:493	chemical analysis	477:493	The raw fruit fibers and extracted cellulose were characterized by chemical analysis.
28115095	6	8	theme	scanning	746:753	arg1	SEM					776:778	SEM	776:778	SEM	776:778	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	8	theme	scanning	746:753	arg1	microscopy					764:773	scanning electron microscopy	746:773	scanning electron microscopy (SEM)	746:779	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	5	9	theme	extraction	623:632	arg1	process					634:640	the cellulose extraction process	609:640	the cellulose extraction process	609:640	The results indicated that the content of the components other than cellulose was significantly decreased during the cellulose extraction process.
28115095	6	10	theme	thermo	708:713	arg1	TGA					737:739	TGA	737:739	TGA	737:739	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	10	theme	thermo	708:713	arg1	analysis					727:734	thermo gravimetric analysis	708:734	thermo gravimetric analysis (TGA)	708:740	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	3	11	theme	cellulose	349:357	arg1	films					359:363	regenerated cellulose films	337:363	regenerated cellulose films	337:363	Using this solution, regenerated cellulose films were prepared with water as the non solvent.
28115095	3	11	theme	cellulose	349:357	arg1	solvent					401:407	the non solvent	393:407	the non solvent	393:407	Using this solution, regenerated cellulose films were prepared with water as the non solvent.
28115095	6	12	theme	cellulose	881:889	arg1	films					891:895	cellulose films	881:895	cellulose films	881:895	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	13	used	used	786:789	arg2	analysis					727:734	thermo gravimetric analysis	708:734	thermo gravimetric analysis (TGA)	708:740	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	13	used	used	786:789	arg2	diffraction					689:699	wide-angle X-ray diffraction	672:699	wide-angle X-ray diffraction (XRD)	672:705	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	13	used	used	786:789	arg2	NMR					667:669	13CP-MAS NMR	658:669	13CP-MAS NMR	658:669	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	13	used	used	786:789	arg2	TGA					737:739	TGA	737:739	TGA	737:739	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	13	used	used	786:789	arg2	XRD					702:704	XRD	702:704	XRD	702:704	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	13	used	used	786:789	arg2	microscopy					764:773	scanning electron microscopy	746:773	scanning electron microscopy (SEM)	746:779	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	13	used	used	786:789	arg2	SEM					776:778	SEM	776:778	SEM	776:778	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	13	used	used	786:789	arg2	FTIR					652:655	FTIR	652:655	FTIR	652:655	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	2	14	theme	1-allyl-3-methylimidazolium	269:295	arg1	chloride					297:304	an ionic liquid 1-allyl-3-methylimidazolium chloride	253:304	an ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl)	253:313	The extracted cellulose was dissolved in an ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl).
28115095	2	14	theme	1-allyl-3-methylimidazolium	269:295	arg1	AmimCl					307:312	AmimCl	307:312	AmimCl	307:312	The extracted cellulose was dissolved in an ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl).
28115095	2	15	theme	liquid	262:267	arg1	chloride					297:304	an ionic liquid 1-allyl-3-methylimidazolium chloride	253:304	an ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl)	253:313	The extracted cellulose was dissolved in an ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl).
28115095	2	15	theme	liquid	262:267	arg1	AmimCl					307:312	AmimCl	307:312	AmimCl	307:312	The extracted cellulose was dissolved in an ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl).
28115095	1	16	theme	borassus	169:176	arg1	fibers					184:189	borassus fruit fibers	169:189	borassus fruit fibers	169:189	In this study, we successfully extracted cellulose from borassus fruit fibers by chemical process.
28115095	1	17	theme	fruit	178:182	arg1	fibers					184:189	borassus fruit fibers	169:189	borassus fruit fibers	169:189	In this study, we successfully extracted cellulose from borassus fruit fibers by chemical process.
28115095	6	18	dep	characterization	813:828	arg1	extracted					845:853	extracted	845:853	extracted cellulose	845:863	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	18	dep	characterization	813:828	arg1	regenerated					869:879	regenerated	869:879	regenerated cellulose films	869:895	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	19	theme	raw	833:835	arg1	fibers					837:842	raw fibers	833:842	raw fibers	833:842	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	20	theme	X-ray	683:687	arg1	diffraction					689:699	wide-angle X-ray diffraction	672:699	wide-angle X-ray diffraction (XRD)	672:705	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	20	theme	X-ray	683:687	arg1	XRD					702:704	XRD	702:704	XRD	702:704	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	1	21	theme	chemical	194:201	arg1	process					203:209	chemical process	194:209	chemical process	194:209	In this study, we successfully extracted cellulose from borassus fruit fibers by chemical process.
28115095	0	22	theme	cellulose	48:56	arg1	films					58:62	regenerated cellulose films	36:62	regenerated cellulose films	36:62	Preparation and characterization of regenerated cellulose films using borassus fruit fibers and an ionic liquid.
28115095	4	23	theme	extracted	435:443	arg1	cellulose					445:453	extracted cellulose	435:453	extracted cellulose	435:453	The raw fruit fibers and extracted cellulose were characterized by chemical analysis.
28115095	6	24	theme	wide-angle	672:681	arg1	diffraction					689:699	wide-angle X-ray diffraction	672:699	wide-angle X-ray diffraction (XRD)	672:705	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	24	theme	wide-angle	672:681	arg1	XRD					702:704	XRD	702:704	XRD	702:704	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	0	25	theme	regenerated	36:46	arg1	films					58:62	regenerated cellulose films	36:62	regenerated cellulose films	36:62	Preparation and characterization of regenerated cellulose films using borassus fruit fibers and an ionic liquid.
28115095	8	26	theme	notable	1067:1073	arg1	properties					1075:1084	The notable properties	1063:1084	The notable properties of regenerated cellulose films	1063:1115	The notable properties of regenerated cellulose films advocate their effectiveness for various industrial applications.
28115095	5	27	theme	components	542:551	arg1	content					527:533	the content	523:533	the content of the components other than cellulose	523:572	The results indicated that the content of the components other than cellulose was significantly decreased during the cellulose extraction process.
28115095	3	28	theme	non	397:399	arg1	solvent					401:407	the non solvent	393:407	the non solvent	393:407	Using this solution, regenerated cellulose films were prepared with water as the non solvent.
28115095	3	28	theme	non	397:399	arg1	films					359:363	regenerated cellulose films	337:363	regenerated cellulose films	337:363	Using this solution, regenerated cellulose films were prepared with water as the non solvent.
28115095	8	29	theme	regenerated	1089:1099	arg1	films					1111:1115	regenerated cellulose films	1089:1115	regenerated cellulose films	1089:1115	The notable properties of regenerated cellulose films advocate their effectiveness for various industrial applications.
28115095	0	30	theme	borassus	70:77	arg1	fibers					85:90	borassus fruit fibers	70:90	borassus fruit fibers	70:90	Preparation and characterization of regenerated cellulose films using borassus fruit fibers and an ionic liquid.
28115095	2	31	theme	extracted	216:224	arg1	cellulose					226:234	The extracted cellulose	212:234	The extracted cellulose	212:234	The extracted cellulose was dissolved in an ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl).
28115095	5	32	theme	other	553:557	arg1	components					542:551	the components	538:551	the components other than cellulose	538:572	The results indicated that the content of the components other than cellulose was significantly decreased during the cellulose extraction process.
28115095	0	33	theme	films	58:62	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of regenerated cellulose films using borassus fruit fibers and an ionic liquid.
28115095	0	33	theme	films	58:62	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of regenerated cellulose films using borassus fruit fibers and an ionic liquid.
28115095	4	34	theme	fruit	418:422	arg1	fibers					424:429	The raw fruit fibers	410:429	The raw fruit fibers	410:429	The raw fruit fibers and extracted cellulose were characterized by chemical analysis.
28115095	7	35	theme	average	902:908	arg1	strength					918:925	The average tensile strength	898:925	The average tensile strength	898:925	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	7	35	theme	average	902:908	arg1	111±19MPa					1012:1020	111±19MPa	1012:1020	111±19MPa	1012:1020	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	8	36	theme	films	1111:1115	arg1	properties					1075:1084	The notable properties	1063:1084	The notable properties of regenerated cellulose films	1063:1115	The notable properties of regenerated cellulose films advocate their effectiveness for various industrial applications.
28115095	4	37	theme	raw	414:416	arg1	fibers					424:429	The raw fruit fibers	410:429	The raw fruit fibers	410:429	The raw fruit fibers and extracted cellulose were characterized by chemical analysis.
28115095	2	38	theme	ionic	256:260	arg1	chloride					297:304	an ionic liquid 1-allyl-3-methylimidazolium chloride	253:304	an ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl)	253:313	The extracted cellulose was dissolved in an ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl).
28115095	2	38	theme	ionic	256:260	arg1	AmimCl					307:312	AmimCl	307:312	AmimCl	307:312	The extracted cellulose was dissolved in an ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl).
28115095	7	39	theme	tensile	910:916	arg1	strength					918:925	The average tensile strength	898:925	The average tensile strength	898:925	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	7	39	theme	tensile	910:916	arg1	111±19MPa					1012:1020	111±19MPa	1012:1020	111±19MPa	1012:1020	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	8	40	theme	cellulose	1101:1109	arg1	films					1111:1115	regenerated cellulose films	1089:1115	regenerated cellulose films	1089:1115	The notable properties of regenerated cellulose films advocate their effectiveness for various industrial applications.
28115095	6	41	theme	electron	755:762	arg1	SEM					776:778	SEM	776:778	SEM	776:778	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	6	41	theme	electron	755:762	arg1	microscopy					764:773	scanning electron microscopy	746:773	scanning electron microscopy (SEM)	746:779	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	7	42	theme	cellulose	979:987	arg1	films					989:993	the regenerated cellulose films	963:993	the regenerated cellulose films	963:993	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	6	43	theme	13CP-MAS	658:665	arg1	NMR					667:669	13CP-MAS NMR	658:669	13CP-MAS NMR	658:669	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	0	44	theme	fruit	79:83	arg1	fibers					85:90	borassus fruit fibers	70:90	borassus fruit fibers	70:90	Preparation and characterization of regenerated cellulose films using borassus fruit fibers and an ionic liquid.
28115095	6	45	theme	structural	802:811	arg1	characterization					813:828	structural characterization	802:828	structural characterization	802:828	Further, FTIR, 13CP-MAS NMR, wide-angle X-ray diffraction (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM) were used to provide structural characterization of raw fibers, extracted cellulose and regenerated cellulose films.
28115095	7	46	theme	films	989:993	arg1	modulus					928:934	modulus	928:934	modulus	928:934	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	7	46	theme	films	989:993	arg1	strength					918:925	The average tensile strength	898:925	The average tensile strength	898:925	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	7	46	theme	films	989:993	arg1	111±19MPa					1012:1020	111±19MPa	1012:1020	111±19MPa	1012:1020	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	7	46	theme	films	989:993	arg1	elongation					940:949	elongation	940:949	elongation	940:949	The average tensile strength, modulus and elongation at break of the regenerated cellulose films were found to be 111±19MPa, 6149±603MPa and 3.1±0.8%, respectively.
28115095	3	47	theme	regenerated	337:347	arg1	films					359:363	regenerated cellulose films	337:363	regenerated cellulose films	337:363	Using this solution, regenerated cellulose films were prepared with water as the non solvent.
28115095	3	47	theme	regenerated	337:347	arg1	solvent					401:407	the non solvent	393:407	the non solvent	393:407	Using this solution, regenerated cellulose films were prepared with water as the non solvent.
26901738	0	0	theme	surface	97:103	arg1	methodology					105:115	response surface methodology	88:115	response surface methodology	88:115	Evaluation of trichloroethylene degradation by starch supported Fe/Ni nanoparticles via response surface methodology.
26901738	4	1	theme	surface	795:801	arg1	RSM					816:818	RSM	816:818	RSM	816:818	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	1	theme	surface	795:801	arg1	methodology					803:813	response surface methodology	786:813	response surface methodology (RSM)	786:819	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	0	2	theme	response	88:95	arg1	methodology					105:115	response surface methodology	88:115	response surface methodology	88:115	Evaluation of trichloroethylene degradation by starch supported Fe/Ni nanoparticles via response surface methodology.
26901738	7	3	theme	dechlorination	1037:1050	arg1	L					1149:1149	initial pH 5.77, Fe(0) dosage 1.67 g L	1112:1149	L	1149:1149	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	3	theme	dechlorination	1037:1050	arg1	conditions					1052:1061	The optimum dechlorination conditions	1025:1061	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1)	1025:1105	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	4	4	theme	response	786:793	arg1	RSM					816:818	RSM	816:818	RSM	816:818	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	4	theme	response	786:793	arg1	methodology					803:813	response surface methodology	786:813	response surface methodology (RSM)	786:819	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	2	5	theme	Fe/Ni	362:366	arg1	nanoparticles					368:380	the Fe/Ni nanoparticles	358:380	the Fe/Ni nanoparticles	358:380	The scanning electron microscope images showed applying water soluble starch as a stabilizer for the Fe/Ni nanoparticles tended to reduce agglomeration and discrete particle.
26901738	4	6	theme	parameters	585:594	arg1	Effects					542:548	Effects	542:548	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour	542:757	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	7	7	theme	initial	1112:1118	arg1	L					1149:1149	initial pH 5.77, Fe(0) dosage 1.67 g L	1112:1149	L	1149:1149	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	7	theme	initial	1112:1118	arg1	conditions					1052:1061	The optimum dechlorination conditions	1025:1061	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1)	1025:1105	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	7	theme	initial	1112:1118	arg1	-1					1151:1152	-1	1151:1152	-1	1151:1152	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	8	theme	optimum	1029:1035	arg1	L					1149:1149	initial pH 5.77, Fe(0) dosage 1.67 g L	1112:1149	L	1149:1149	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	8	theme	optimum	1029:1035	arg1	conditions					1052:1061	The optimum dechlorination conditions	1025:1061	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1)	1025:1105	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	2	9	theme	applying	308:315	arg1	starch					331:336	applying water soluble starch	308:336	applying water soluble starch	308:336	The scanning electron microscope images showed applying water soluble starch as a stabilizer for the Fe/Ni nanoparticles tended to reduce agglomeration and discrete particle.
26901738	4	10	theme	TCE	614:616	arg1	L					647:647	10.0-300.0 mg L(-1)	633:651	10.0-300.0 mg L(-1)	633:651	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	10	theme	TCE	614:616	arg1	concentration					618:630	initial TCE concentration	606:630	initial TCE concentration (10.0-300.0 mg L(-1))	606:652	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	7	11	theme	pH	1120:1121	arg1	L					1149:1149	initial pH 5.77, Fe(0) dosage 1.67 g L	1112:1149	L	1149:1149	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	11	theme	pH	1120:1121	arg1	conditions					1052:1061	The optimum dechlorination conditions	1025:1061	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1)	1025:1105	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	11	theme	pH	1120:1121	arg1	-1					1151:1152	-1	1151:1152	-1	1151:1152	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	5	12	theme	removal	887:893	arg1	efficiency					895:904	TCE removal efficiency	883:904	TCE removal efficiency	883:904	Based on a five-level three-factor central composite design, TCE removal efficiency was examined and optimized.
26901738	4	13	theme	g	708:708	arg1	L					710:710	Fe(0) dosage (0.10-2.00) g L	683:710	Fe(0) dosage (0.10-2.00) g L(-1)	683:714	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	13	theme	g	708:708	arg1	-1					712:713	-1	712:713	-1	712:713	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	14	theme	initial	606:612	arg1	L					647:647	10.0-300.0 mg L(-1)	633:651	10.0-300.0 mg L(-1)	633:651	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	14	theme	initial	606:612	arg1	concentration					618:630	initial TCE concentration	606:630	initial TCE concentration (10.0-300.0 mg L(-1))	606:652	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	8	15	theme	predicted	1252:1260	arg1	value					1262:1266	predicted value	1252:1266	predicted value	1252:1266	At these conditions TCE removal concentration reached 94.87%, which is in close acceptance with predicted value by the RSM model.
26901738	6	16	theme	order	998:1002	arg1	equation					1015:1022	a second order polynomial equation	989:1022	a second order polynomial equation	989:1022	The obtained RSM model fitted the experimental data to a second order polynomial equation.
26901738	1	17	theme	chlorinated	175:185	arg1	hydrocarbon					187:197	a chlorinated hydrocarbon	173:197	a chlorinated hydrocarbon	173:197	In this study, degradation of trichloroethylene (TCE), a chlorinated hydrocarbon, using starch supported Fe/Ni nanoparticles was investigated.
26901738	1	17	theme	chlorinated	175:185	arg1	trichloroethylene					148:164	trichloroethylene	148:164	trichloroethylene (TCE)	148:170	In this study, degradation of trichloroethylene (TCE), a chlorinated hydrocarbon, using starch supported Fe/Ni nanoparticles was investigated.
26901738	2	18	theme	discrete	417:424	arg1	particle					426:433	discrete particle	417:433	discrete particle	417:433	The scanning electron microscope images showed applying water soluble starch as a stabilizer for the Fe/Ni nanoparticles tended to reduce agglomeration and discrete particle.
26901738	2	19	theme	microscope	283:292	arg1	images					294:299	The scanning electron microscope images	261:299	The scanning electron microscope images	261:299	The scanning electron microscope images showed applying water soluble starch as a stabilizer for the Fe/Ni nanoparticles tended to reduce agglomeration and discrete particle.
26901738	7	20	dep	L	1149:1149	arg1	Fe					1129:1130	initial pH 5.77, Fe(0) dosage 1.67 g L	1112:1149	Fe	1129:1130	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	6	21	theme	second	991:996	arg1	equation					1015:1022	a second order polynomial equation	989:1022	a second order polynomial equation	989:1022	The obtained RSM model fitted the experimental data to a second order polynomial equation.
26901738	8	22	with	acceptance	1236:1245	arg1	value					1262:1266	predicted value	1252:1266	predicted value	1252:1266	At these conditions TCE removal concentration reached 94.87%, which is in close acceptance with predicted value by the RSM model.
26901738	7	23	dep	concentration	1078:1090	arg1	L					1101:1101	100.0 mg L	1092:1101	initial TCE concentration 100.0 mg L(-1)	1066:1105	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	23	dep	concentration	1078:1090	arg1	-1					1103:1104	-1	1103:1104	-1	1103:1104	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	5	24	theme	central	857:863	arg1	design					875:880	a five-level three-factor central composite design	831:880	a five-level three-factor central composite design	831:880	Based on a five-level three-factor central composite design, TCE removal efficiency was examined and optimized.
26901738	3	25	theme	mean	445:448	arg1	diameter					459:466	the mean particle diameter	441:466	the mean particle diameter	441:466	Also the mean particle diameter reduced from about 70 nm (unsupported Fe/Ni nanoparticle) to about 30 nm.
26901738	7	26	from	concentration	1078:1090	arg1	L					1149:1149	initial pH 5.77, Fe(0) dosage 1.67 g L	1112:1149	L	1149:1149	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	26	from	concentration	1078:1090	arg1	conditions					1052:1061	The optimum dechlorination conditions	1025:1061	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1)	1025:1105	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	4	27	theme	key	559:561	arg1	L					710:710	Fe(0) dosage (0.10-2.00) g L	683:710	Fe(0) dosage (0.10-2.00) g L(-1)	683:714	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	27	theme	key	559:561	arg1	parameters					585:594	three key independent operating parameters	553:594	three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1)	553:714	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	27	theme	key	559:561	arg1	concentration					618:630	initial TCE concentration	606:630	initial TCE concentration (10.0-300.0 mg L(-1))	606:652	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	27	theme	key	559:561	arg1	pH					663:664	initial pH	655:664	initial pH (4.00-10.00)	655:677	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	28	theme	dosage	689:694	arg1	L					710:710	Fe(0) dosage (0.10-2.00) g L	683:710	Fe(0) dosage (0.10-2.00) g L(-1)	683:714	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	28	theme	dosage	689:694	arg1	-1					712:713	-1	712:713	-1	712:713	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	3	29	theme	particle	450:457	arg1	diameter					459:466	the mean particle diameter	441:466	the mean particle diameter	441:466	Also the mean particle diameter reduced from about 70 nm (unsupported Fe/Ni nanoparticle) to about 30 nm.
26901738	0	30	theme	degradation	32:42	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of trichloroethylene degradation by starch	0:52	Evaluation of trichloroethylene degradation by starch supported Fe/Ni nanoparticles via response surface methodology.
26901738	5	31	theme	three-factor	844:855	arg1	design					875:880	a five-level three-factor central composite design	831:880	a five-level three-factor central composite design	831:880	Based on a five-level three-factor central composite design, TCE removal efficiency was examined and optimized.
26901738	2	32	theme	soluble	323:329	arg1	starch					331:336	applying water soluble starch	308:336	applying water soluble starch	308:336	The scanning electron microscope images showed applying water soluble starch as a stabilizer for the Fe/Ni nanoparticles tended to reduce agglomeration and discrete particle.
26901738	5	33	theme	TCE	883:885	arg1	efficiency					895:904	TCE removal efficiency	883:904	TCE removal efficiency	883:904	Based on a five-level three-factor central composite design, TCE removal efficiency was examined and optimized.
26901738	0	34	theme	trichloroethylene	14:30	arg1	degradation					32:42	trichloroethylene degradation	14:42	trichloroethylene degradation	14:42	Evaluation of trichloroethylene degradation by starch supported Fe/Ni nanoparticles via response surface methodology.
26901738	4	35	theme	operating	575:583	arg1	L					710:710	Fe(0) dosage (0.10-2.00) g L	683:710	Fe(0) dosage (0.10-2.00) g L(-1)	683:714	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	35	theme	operating	575:583	arg1	parameters					585:594	three key independent operating parameters	553:594	three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1)	553:714	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	35	theme	operating	575:583	arg1	concentration					618:630	initial TCE concentration	606:630	initial TCE concentration (10.0-300.0 mg L(-1))	606:652	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	35	theme	operating	575:583	arg1	pH					663:664	initial pH	655:664	initial pH (4.00-10.00)	655:677	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	5	36	theme	composite	865:873	arg1	design					875:880	a five-level three-factor central composite design	831:880	a five-level three-factor central composite design	831:880	Based on a five-level three-factor central composite design, TCE removal efficiency was examined and optimized.
26901738	6	37	theme	polynomial	1004:1013	arg1	equation					1015:1022	a second order polynomial equation	989:1022	a second order polynomial equation	989:1022	The obtained RSM model fitted the experimental data to a second order polynomial equation.
26901738	6	38	theme	experimental	968:979	arg1	data					981:984	the experimental data	964:984	the experimental data	964:984	The obtained RSM model fitted the experimental data to a second order polynomial equation.
26901738	4	39	theme	independent	563:573	arg1	L					710:710	Fe(0) dosage (0.10-2.00) g L	683:710	Fe(0) dosage (0.10-2.00) g L(-1)	683:714	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	39	theme	independent	563:573	arg1	parameters					585:594	three key independent operating parameters	553:594	three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1)	553:714	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	39	theme	independent	563:573	arg1	concentration					618:630	initial TCE concentration	606:630	initial TCE concentration (10.0-300.0 mg L(-1))	606:652	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	39	theme	independent	563:573	arg1	pH					663:664	initial pH	655:664	initial pH (4.00-10.00)	655:677	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	7	40	theme	dosage	1135:1140	arg1	L					1149:1149	initial pH 5.77, Fe(0) dosage 1.67 g L	1112:1149	L	1149:1149	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	40	theme	dosage	1135:1140	arg1	conditions					1052:1061	The optimum dechlorination conditions	1025:1061	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1)	1025:1105	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	40	theme	dosage	1135:1140	arg1	-1					1151:1152	-1	1151:1152	-1	1151:1152	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	8	41	theme	TCE	1176:1178	arg1	concentration					1188:1200	TCE removal concentration	1176:1200	TCE removal concentration	1176:1200	At these conditions TCE removal concentration reached 94.87%, which is in close acceptance with predicted value by the RSM model.
26901738	4	42	theme	initial	655:661	arg1	4.00-10.00					667:676	4.00-10.00	667:676	4.00-10.00	667:676	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	42	theme	initial	655:661	arg1	pH					663:664	initial pH	655:664	initial pH (4.00-10.00)	655:677	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	1	43	theme	starch	206:211	arg1	nanoparticles					229:241	starch supported Fe/Ni nanoparticles	206:241	starch supported Fe/Ni nanoparticles	206:241	In this study, degradation of trichloroethylene (TCE), a chlorinated hydrocarbon, using starch supported Fe/Ni nanoparticles was investigated.
26901738	4	44	from	Effects	542:548	arg1	efficiency					738:747	TCE dechlorination efficiency	719:747	TCE dechlorination efficiency in 1 hour	719:757	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	6	45	theme	obtained	938:945	arg1	model					951:955	The obtained RSM model	934:955	The obtained RSM model	934:955	The obtained RSM model fitted the experimental data to a second order polynomial equation.
26901738	1	46	theme	supported	213:221	arg1	nanoparticles					229:241	starch supported Fe/Ni nanoparticles	206:241	starch supported Fe/Ni nanoparticles	206:241	In this study, degradation of trichloroethylene (TCE), a chlorinated hydrocarbon, using starch supported Fe/Ni nanoparticles was investigated.
26901738	0	47	theme	Fe/Ni	64:68	arg1	nanoparticles					70:82	Fe/Ni nanoparticles	64:82	Fe/Ni nanoparticles	64:82	Evaluation of trichloroethylene degradation by starch supported Fe/Ni nanoparticles via response surface methodology.
26901738	3	48	theme	Fe/Ni	506:510	arg1	nanoparticle					512:523	unsupported Fe/Ni nanoparticle	494:523	unsupported Fe/Ni nanoparticle	494:523	Also the mean particle diameter reduced from about 70 nm (unsupported Fe/Ni nanoparticle) to about 30 nm.
26901738	3	48	theme	Fe/Ni	506:510	arg1	nm					490:491	about 70 nm	481:491	about 70 nm (unsupported Fe/Ni nanoparticle)	481:524	Also the mean particle diameter reduced from about 70 nm (unsupported Fe/Ni nanoparticle) to about 30 nm.
26901738	7	49	theme	TCE	1074:1076	arg1	concentration					1078:1090	initial TCE concentration	1066:1090	initial TCE concentration 100.0 mg L(-1)	1066:1105	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	8	50	theme	removal	1180:1186	arg1	concentration					1188:1200	TCE removal concentration	1176:1200	TCE removal concentration	1176:1200	At these conditions TCE removal concentration reached 94.87%, which is in close acceptance with predicted value by the RSM model.
26901738	8	51	theme	RSM	1275:1277	arg1	model					1279:1283	the RSM model	1271:1283	the RSM model	1271:1283	At these conditions TCE removal concentration reached 94.87%, which is in close acceptance with predicted value by the RSM model.
26901738	4	52	theme	Fe	683:684	arg1	L					710:710	Fe(0) dosage (0.10-2.00) g L	683:710	Fe(0) dosage (0.10-2.00) g L(-1)	683:714	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	52	theme	Fe	683:684	arg1	-1					712:713	-1	712:713	-1	712:713	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	7	53	theme	mg	1098:1099	arg1	L					1101:1101	100.0 mg L	1092:1101	initial TCE concentration 100.0 mg L(-1)	1066:1105	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	53	theme	mg	1098:1099	arg1	-1					1103:1104	-1	1103:1104	-1	1103:1104	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	2	54	theme	electron	274:281	arg1	images					294:299	The scanning electron microscope images	261:299	The scanning electron microscope images	261:299	The scanning electron microscope images showed applying water soluble starch as a stabilizer for the Fe/Ni nanoparticles tended to reduce agglomeration and discrete particle.
26901738	4	55	from	efficiency	738:747	arg1	hour					754:757	1 hour	752:757	1 hour	752:757	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	5	56	theme	five-level	833:842	arg1	design					875:880	a five-level three-factor central composite design	831:880	a five-level three-factor central composite design	831:880	Based on a five-level three-factor central composite design, TCE removal efficiency was examined and optimized.
26901738	8	57	theme	close	1230:1234	arg1	acceptance					1236:1245	close acceptance	1230:1245	close acceptance with predicted value	1230:1266	At these conditions TCE removal concentration reached 94.87%, which is in close acceptance with predicted value by the RSM model.
26901738	1	58	theme	Fe/Ni	223:227	arg1	nanoparticles					229:241	starch supported Fe/Ni nanoparticles	206:241	starch supported Fe/Ni nanoparticles	206:241	In this study, degradation of trichloroethylene (TCE), a chlorinated hydrocarbon, using starch supported Fe/Ni nanoparticles was investigated.
26901738	2	59	theme	scanning	265:272	arg1	images					294:299	The scanning electron microscope images	261:299	The scanning electron microscope images	261:299	The scanning electron microscope images showed applying water soluble starch as a stabilizer for the Fe/Ni nanoparticles tended to reduce agglomeration and discrete particle.
26901738	3	60	theme	unsupported	494:504	arg1	nanoparticle					512:523	unsupported Fe/Ni nanoparticle	494:523	unsupported Fe/Ni nanoparticle	494:523	Also the mean particle diameter reduced from about 70 nm (unsupported Fe/Ni nanoparticle) to about 30 nm.
26901738	3	60	theme	unsupported	494:504	arg1	nm					490:491	about 70 nm	481:491	about 70 nm (unsupported Fe/Ni nanoparticle)	481:524	Also the mean particle diameter reduced from about 70 nm (unsupported Fe/Ni nanoparticle) to about 30 nm.
26901738	6	61	theme	RSM	947:949	arg1	model					951:955	The obtained RSM model	934:955	The obtained RSM model	934:955	The obtained RSM model fitted the experimental data to a second order polynomial equation.
26901738	4	62	theme	dechlorination	723:736	arg1	efficiency					738:747	TCE dechlorination efficiency	719:747	TCE dechlorination efficiency in 1 hour	719:757	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	1	63	theme	trichloroethylene	148:164	arg1	degradation					133:143	degradation	133:143	degradation of trichloroethylene (TCE), a chlorinated hydrocarbon, using starch supported Fe/Ni nanoparticles	133:241	In this study, degradation of trichloroethylene (TCE), a chlorinated hydrocarbon, using starch supported Fe/Ni nanoparticles was investigated.
26901738	4	64	theme	mg	644:645	arg1	L					647:647	10.0-300.0 mg L(-1)	633:651	10.0-300.0 mg L(-1)	633:651	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	4	64	theme	mg	644:645	arg1	concentration					618:630	initial TCE concentration	606:630	initial TCE concentration (10.0-300.0 mg L(-1))	606:652	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	7	65	theme	initial	1066:1072	arg1	concentration					1078:1090	initial TCE concentration	1066:1090	initial TCE concentration 100.0 mg L(-1)	1066:1105	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	4	66	theme	TCE	719:721	arg1	efficiency					738:747	TCE dechlorination efficiency	719:747	TCE dechlorination efficiency in 1 hour	719:757	Effects of three key independent operating parameters including initial TCE concentration (10.0-300.0 mg L(-1)), initial pH (4.00-10.00) and Fe(0) dosage (0.10-2.00) g L(-1) on TCE dechlorination efficiency in 1 hour were analysed by employing response surface methodology (RSM).
26901738	7	67	theme	g	1147:1147	arg1	L					1149:1149	initial pH 5.77, Fe(0) dosage 1.67 g L	1112:1149	L	1149:1149	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	67	theme	g	1147:1147	arg1	conditions					1052:1061	The optimum dechlorination conditions	1025:1061	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1)	1025:1105	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
26901738	7	67	theme	g	1147:1147	arg1	-1					1151:1152	-1	1151:1152	-1	1151:1152	The optimum dechlorination conditions at initial TCE concentration 100.0 mg L(-1) were initial pH 5.77, Fe(0) dosage 1.67 g L(-1).
25063173	6	0	theme	carboxyl	892:899	arg1	chain					924:928	stearate side chain	910:928	stearate side chain after DHT	910:938	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	6	0	theme	carboxyl	892:899	arg1	chitosan					879:886	chitosan	879:886	chitosan	879:886	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	6	0	theme	carboxyl	892:899	arg1	group					901:905	carboxyl group	892:905	carboxyl group of stearate side chain after DHT	892:938	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	2	1	from	ion	404:406	arg1	acid					425:428	2% w/v lactic acid	411:428	2% w/v lactic acid	411:428	Alst was able to dissociate and to cleave stearate ion in 2% w/v lactic acid.
25063173	13	2	theme	power	1784:1788	arg1	law					1790:1792	power law	1784:1792	power law model	1784:1798	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	1	3	theme	chitosan	263:270	arg1	hydrophobicity					245:258	hydrophobicity	245:258	hydrophobicity of chitosan	245:270	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	6	4	theme	chitosan	879:886	arg1	group					870:874	amino group	864:874	amino group of chitosan	864:886	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	6	4	theme	chitosan	879:886	arg1	chain					924:928	stearate side chain	910:928	stearate side chain after DHT	910:938	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	6	4	theme	chitosan	879:886	arg1	chitosan					879:886	chitosan	879:886	chitosan	879:886	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	6	4	theme	chitosan	879:886	arg1	group					901:905	carboxyl group	892:905	carboxyl group of stearate side chain after DHT	892:938	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	4	5	theme	pH	546:547	arg1	value					549:553	The pH value	542:553	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration	542:675	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
25063173	13	6	theme	exponent	1762:1769	arg1	value					1771:1775	the exponent value	1758:1775	the exponent value (n) in power law model	1758:1798	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	13	6	theme	exponent	1762:1769	arg1	n					1778:1778	n	1778:1778	n	1778:1778	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	13	7	theme	law	1790:1792	arg1	model					1794:1798	power law model	1784:1798	power law model	1784:1798	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	8	8	theme	phosphate	1158:1166	arg1	pH7.4					1176:1180	pH7.4	1176:1180	pH7.4	1176:1180	Swelling behavior of the sponges was investigated in three different medium namely acetate buffer (pH4.0), phosphate buffer (pH7.4) and carbonate buffer (pH10.0).
25063173	8	8	theme	phosphate	1158:1166	arg1	buffer					1168:1173	phosphate buffer	1158:1173	phosphate buffer (pH7.4)	1158:1181	Swelling behavior of the sponges was investigated in three different medium namely acetate buffer (pH4.0), phosphate buffer (pH7.4) and carbonate buffer (pH10.0).
25063173	10	9	theme	DHT	1381:1383	arg1	addition					1360:1367	addition	1360:1367	addition of Alst and DHT	1360:1383	Contact angle measurement revealed that addition of Alst and DHT enhanced the hydrophobicity of the materials.
25063173	1	10	theme	chitosan	175:182	arg1	sponges					184:190	hydrophobic chitosan sponges	163:190	hydrophobic chitosan sponges	163:190	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	4	11	theme	complex	617:623	arg1	dispersions					566:576	CLA dispersions	562:576	CLA dispersions	562:576	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
25063173	4	11	theme	complex	617:623	arg1	size					591:594	particle size	582:594	particle size	582:594	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
25063173	10	12	theme	Alst	1372:1375	arg1	addition					1360:1367	addition	1360:1367	addition of Alst and DHT	1360:1383	Contact angle measurement revealed that addition of Alst and DHT enhanced the hydrophobicity of the materials.
25063173	3	13	theme	room	524:527	arg1	temperature					529:539	room temperature	524:539	room temperature	524:539	Composite dispersion of chitosan and Alst (CLA) could be easily prepared by simple mixing at room temperature.
25063173	12	14	theme	asiaticoside	1559:1570	arg1	release					1548:1554	the release	1544:1554	the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character	1544:1673	Swelling behavior of the sponges was coincident with the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character.
25063173	12	15	with	coincident	1528:1537	arg1	release					1548:1554	the release	1544:1554	the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character	1544:1673	Swelling behavior of the sponges was coincident with the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character.
25063173	13	16	from	value	1771:1775	arg1	model					1794:1798	power law model	1784:1798	power law model	1784:1798	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	14	17	dep	drug-sustained-release	1977:1998	arg1	porous					2001:2006	porous	2001:2006	porous	2001:2006	From this study, we found the potential of the prepared hydrophobic chitosan sponges for further application as drug-sustained-release, porous wound dressing.
25063173	1	18	theme	novel	201:205	arg1	technique					226:234	novel simple preparation technique	201:234	novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT)	201:350	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	8	19	theme	carbonate	1187:1195	arg1	pH10.0					1205:1210	pH10.0	1205:1210	pH10.0	1205:1210	Swelling behavior of the sponges was investigated in three different medium namely acetate buffer (pH4.0), phosphate buffer (pH7.4) and carbonate buffer (pH10.0).
25063173	8	19	theme	carbonate	1187:1195	arg1	buffer					1197:1202	carbonate buffer	1187:1202	carbonate buffer (pH10.0)	1187:1211	Swelling behavior of the sponges was investigated in three different medium namely acetate buffer (pH4.0), phosphate buffer (pH7.4) and carbonate buffer (pH10.0).
25063173	4	20	theme	size	591:594	arg1	value					549:553	The pH value	542:553	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration	542:675	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
25063173	7	21	theme	hydrophilic/hydrophobic	991:1013	arg1	properties					1015:1024	hydrophilic/hydrophobic properties	991:1024	hydrophilic/hydrophobic properties of the prepared sponges	991:1048	Contact angle measurement was applied to evaluate hydrophilic/hydrophobic properties of the prepared sponges.
25063173	1	22	theme	preparation	214:224	arg1	technique					226:234	novel simple preparation technique	201:234	novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT)	201:350	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	6	23	theme	side	919:922	arg1	chain					924:928	stearate side chain	910:928	stearate side chain after DHT	910:938	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	4	24	theme	chitosan	654:661	arg1	concentration					663:675	low chitosan concentration	650:675	low chitosan concentration	650:675	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
25063173	7	25	theme	sponges	1042:1048	arg1	properties					1015:1024	hydrophilic/hydrophobic properties	991:1024	hydrophilic/hydrophobic properties of the prepared sponges	991:1048	Contact angle measurement was applied to evaluate hydrophilic/hydrophobic properties of the prepared sponges.
25063173	9	26	theme	release	1219:1225	arg1	study					1227:1231	Drug release study	1214:1231	Drug release study	1214:1231	Drug release study was conducted in phosphate buffer pH7.4 at 37°C by using asiaticoside as a model drug.
25063173	0	27	theme	dehydrothermal	67:80	arg1	treatment					82:90	dehydrothermal treatment	67:90	dehydrothermal treatment	67:90	Hydrophobic chitosan sponges modified by aluminum monostearate and dehydrothermal treatment as sustained drug delivery system.
25063173	7	28	theme	angle	949:953	arg1	measurement					955:965	Contact angle measurement	941:965	Contact angle measurement	941:965	Contact angle measurement was applied to evaluate hydrophilic/hydrophobic properties of the prepared sponges.
25063173	12	29	theme	release	1657:1663	arg1	character					1665:1673	the sustained release character	1643:1673	the sustained release character	1643:1673	Swelling behavior of the sponges was coincident with the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character.
25063173	13	30	theme	Fickian	1846:1852	arg1	diffusion					1854:1862	Fickian diffusion	1846:1862	Fickian diffusion	1846:1862	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	13	30	theme	Fickian	1846:1852	arg1	mechanism					1832:1840	the main release mechanism	1815:1840	the main release mechanism	1815:1840	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	4	31	from	value	549:553	arg1	system					632:637	the system	628:637	the system comprising low chitosan concentration	628:675	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
25063173	13	32	theme	asiaticoside	1687:1698	arg1	Release					1676:1682	Release	1676:1682	Release of asiaticoside from CLA sponges	1676:1715	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	11	33	theme	Alst	1468:1471	arg1	amount					1473:1478	Alst amount	1468:1478	Alst amount	1468:1478	Swelling of the sponges decreased as Alst amount increased.
25063173	0	34	theme	drug	105:108	arg1	system					119:124	sustained drug delivery system	95:124	sustained drug delivery system	95:124	Hydrophobic chitosan sponges modified by aluminum monostearate and dehydrothermal treatment as sustained drug delivery system.
25063173	14	35	theme	drug-sustained-release	1977:1998	arg1	dressing					2014:2021	drug-sustained-release, porous wound dressing	1977:2021	drug-sustained-release, porous wound dressing	1977:2021	From this study, we found the potential of the prepared hydrophobic chitosan sponges for further application as drug-sustained-release, porous wound dressing.
25063173	2	36	dep	able	362:365	arg1	dissociate					370:379	dissociate	370:379	to dissociate	367:379	Alst was able to dissociate and to cleave stearate ion in 2% w/v lactic acid.
25063173	2	36	dep	able	362:365	arg1	cleave					388:393	cleave	388:393	to cleave stearate ion in 2% w/v lactic acid	385:428	Alst was able to dissociate and to cleave stearate ion in 2% w/v lactic acid.
25063173	13	37	theme	main	1819:1822	arg1	diffusion					1854:1862	Fickian diffusion	1846:1862	Fickian diffusion	1846:1862	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	13	37	theme	main	1819:1822	arg1	mechanism					1832:1840	the main release mechanism	1815:1840	the main release mechanism	1815:1840	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	2	38	theme	lactic	418:423	arg1	acid					425:428	2% w/v lactic acid	411:428	2% w/v lactic acid	411:428	Alst was able to dissociate and to cleave stearate ion in 2% w/v lactic acid.
25063173	10	39	theme	materials	1420:1428	arg1	hydrophobicity					1398:1411	the hydrophobicity	1394:1411	the hydrophobicity of the materials	1394:1428	Contact angle measurement revealed that addition of Alst and DHT enhanced the hydrophobicity of the materials.
25063173	0	40	theme	Hydrophobic	0:10	arg1	sponges					21:27	Hydrophobic chitosan sponges	0:27	Hydrophobic chitosan sponges	0:27	Hydrophobic chitosan sponges modified by aluminum monostearate and dehydrothermal treatment as sustained drug delivery system.
25063173	2	41	theme	%	412:412	arg1	acid					425:428	2% w/v lactic acid	411:428	2% w/v lactic acid	411:428	Alst was able to dissociate and to cleave stearate ion in 2% w/v lactic acid.
25063173	3	42	theme	Composite	431:439	arg1	dispersion					441:450	Composite dispersion	431:450	Composite dispersion of chitosan and Alst (CLA)	431:477	Composite dispersion of chitosan and Alst (CLA) could be easily prepared by simple mixing at room temperature.
25063173	14	43	theme	chitosan	1933:1940	arg1	sponges					1942:1948	the prepared hydrophobic chitosan sponges	1908:1948	the prepared hydrophobic chitosan sponges	1908:1948	From this study, we found the potential of the prepared hydrophobic chitosan sponges for further application as drug-sustained-release, porous wound dressing.
25063173	12	44	theme	Alst	1610:1613	arg1	amount					1615:1620	higher Alst amount	1603:1620	higher Alst amount	1603:1620	Swelling behavior of the sponges was coincident with the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character.
25063173	2	45	theme	stearate	395:402	arg1	ion					404:406	stearate ion	395:406	stearate ion in 2% w/v lactic acid	395:428	Alst was able to dissociate and to cleave stearate ion in 2% w/v lactic acid.
25063173	14	46	theme	prepared	1912:1919	arg1	sponges					1942:1948	the prepared hydrophobic chitosan sponges	1908:1948	the prepared hydrophobic chitosan sponges	1908:1948	From this study, we found the potential of the prepared hydrophobic chitosan sponges for further application as drug-sustained-release, porous wound dressing.
25063173	6	47	theme	FT-IR	813:817	arg1	spectra					819:825	FT-IR spectra	813:825	FT-IR spectra analysis	813:834	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	4	48	dep	dispersions	566:576	arg1	the					558:560	the	558:560	the	558:560	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
25063173	14	49	theme	wound	2008:2012	arg1	dressing					2014:2021	drug-sustained-release, porous wound dressing	1977:2021	drug-sustained-release, porous wound dressing	1977:2021	From this study, we found the potential of the prepared hydrophobic chitosan sponges for further application as drug-sustained-release, porous wound dressing.
25063173	8	50	theme	different	1110:1118	arg1	medium					1120:1125	three different medium	1104:1125	three different medium	1104:1125	Swelling behavior of the sponges was investigated in three different medium namely acetate buffer (pH4.0), phosphate buffer (pH7.4) and carbonate buffer (pH10.0).
25063173	10	51	theme	Contact	1320:1326	arg1	measurement					1334:1344	Contact angle measurement	1320:1344	Contact angle measurement	1320:1344	Contact angle measurement revealed that addition of Alst and DHT enhanced the hydrophobicity of the materials.
25063173	12	52	contain	containing	1592:1601	arg1	sponge					1585:1590	the sponge	1581:1590	the sponge containing higher Alst amount	1581:1620	Swelling behavior of the sponges was coincident with the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character.
25063173	12	52	contain	containing	1592:1601	arg2	amount					1615:1620	higher Alst amount	1603:1620	higher Alst amount	1603:1620	Swelling behavior of the sponges was coincident with the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character.
25063173	1	53	theme	hydrophobic	163:173	arg1	sponges					184:190	hydrophobic chitosan sponges	163:190	hydrophobic chitosan sponges	163:190	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	6	54	theme	amino	864:868	arg1	group					870:874	amino group	864:874	amino group of chitosan	864:886	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	6	54	theme	amino	864:868	arg1	chain					924:928	stearate side chain	910:928	stearate side chain after DHT	910:938	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	6	54	theme	amino	864:868	arg1	chitosan					879:886	chitosan	879:886	chitosan	879:886	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	8	55	dep	medium	1120:1125	arg1	buffer					1142:1147	acetate buffer	1134:1147	acetate buffer (pH4.0)	1134:1155	Swelling behavior of the sponges was investigated in three different medium namely acetate buffer (pH4.0), phosphate buffer (pH7.4) and carbonate buffer (pH10.0).
25063173	8	55	dep	medium	1120:1125	arg1	pH4.0					1150:1154	pH4.0	1150:1154	pH4.0	1150:1154	Swelling behavior of the sponges was investigated in three different medium namely acetate buffer (pH4.0), phosphate buffer (pH7.4) and carbonate buffer (pH10.0).
25063173	4	56	theme	chitosan-Alst	603:615	arg1	complex					617:623	the chitosan-Alst complex	599:623	the chitosan-Alst complex	599:623	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
25063173	3	57	theme	simple	507:512	arg1	mixing					514:519	simple mixing	507:519	simple mixing at room temperature	507:539	Composite dispersion of chitosan and Alst (CLA) could be easily prepared by simple mixing at room temperature.
25063173	9	58	from	37°C	1276:1279	arg1	buffer					1260:1265	phosphate buffer pH7.4	1250:1271	phosphate buffer pH7.4 at 37°C	1250:1279	Drug release study was conducted in phosphate buffer pH7.4 at 37°C by using asiaticoside as a model drug.
25063173	4	59	theme	dispersions	566:576	arg1	value					549:553	The pH value	542:553	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration	542:675	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
25063173	13	60	theme	CLA	1705:1707	arg1	sponges					1709:1715	CLA sponges	1705:1715	CLA sponges	1705:1715	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	1	61	theme	simple	207:212	arg1	technique					226:234	novel simple preparation technique	201:234	novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT)	201:350	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	1	62	mod	modified	276:283	arg3	treatment					336:344	dehydrothermal treatment	321:344	dehydrothermal treatment (DHT)	321:350	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	1	62	mod	modified	276:283	arg3	monostearate					297:308	aluminum monostearate	288:308	aluminum monostearate (Alst)	288:315	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	1	62	mod	modified	276:283	arg1	hydrophobicity					245:258	hydrophobicity	245:258	hydrophobicity of chitosan	245:270	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	1	62	mod	modified	276:283	arg3	Alst					311:314	Alst	311:314	Alst	311:314	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	1	62	mod	modified	276:283	arg3	DHT					347:349	DHT	347:349	DHT	347:349	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	4	63	theme	particle	582:589	arg1	size					591:594	particle size	582:594	particle size	582:594	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
25063173	7	64	theme	prepared	1033:1040	arg1	sponges					1042:1048	the prepared sponges	1029:1048	the prepared sponges	1029:1048	Contact angle measurement was applied to evaluate hydrophilic/hydrophobic properties of the prepared sponges.
25063173	9	65	theme	Drug	1214:1217	arg1	study					1227:1231	Drug release study	1214:1231	Drug release study	1214:1231	Drug release study was conducted in phosphate buffer pH7.4 at 37°C by using asiaticoside as a model drug.
25063173	6	66	theme	stearate	910:917	arg1	chain					924:928	stearate side chain	910:928	stearate side chain after DHT	910:938	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	4	67	theme	low	650:652	arg1	concentration					663:675	low chitosan concentration	650:675	low chitosan concentration	650:675	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
25063173	11	68	theme	sponges	1447:1453	arg1	Swelling					1431:1438	Swelling	1431:1438	Swelling of the sponges	1431:1453	Swelling of the sponges decreased as Alst amount increased.
25063173	12	69	theme	sponges	1516:1522	arg1	behavior					1500:1507	Swelling behavior	1491:1507	Swelling behavior of the sponges	1491:1522	Swelling behavior of the sponges was coincident with the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character.
25063173	1	70	theme	study	143:147	arg1	aim					131:133	The aim	127:133	The aim of this study	127:147	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	0	71	theme	sustained	95:103	arg1	system					119:124	sustained drug delivery system	95:124	sustained drug delivery system	95:124	Hydrophobic chitosan sponges modified by aluminum monostearate and dehydrothermal treatment as sustained drug delivery system.
25063173	9	72	theme	phosphate	1250:1258	arg1	buffer					1260:1265	phosphate buffer pH7.4	1250:1271	phosphate buffer pH7.4 at 37°C	1250:1279	Drug release study was conducted in phosphate buffer pH7.4 at 37°C by using asiaticoside as a model drug.
25063173	12	73	theme	sustained	1647:1655	arg1	character					1665:1673	the sustained release character	1643:1673	the sustained release character	1643:1673	Swelling behavior of the sponges was coincident with the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character.
25063173	12	74	theme	Swelling	1491:1498	arg1	behavior					1500:1507	Swelling behavior	1491:1507	Swelling behavior of the sponges	1491:1522	Swelling behavior of the sponges was coincident with the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character.
25063173	0	75	theme	delivery	110:117	arg1	system					119:124	sustained drug delivery system	95:124	sustained drug delivery system	95:124	Hydrophobic chitosan sponges modified by aluminum monostearate and dehydrothermal treatment as sustained drug delivery system.
25063173	13	76	from	sponges	1709:1715	arg1	Release					1676:1682	Release	1676:1682	Release of asiaticoside from CLA sponges	1676:1715	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	7	77	theme	Contact	941:947	arg1	measurement					955:965	Contact angle measurement	941:965	Contact angle measurement	941:965	Contact angle measurement was applied to evaluate hydrophilic/hydrophobic properties of the prepared sponges.
25063173	8	78	theme	Swelling	1051:1058	arg1	behavior					1060:1067	Swelling behavior	1051:1067	Swelling behavior of the sponges	1051:1082	Swelling behavior of the sponges was investigated in three different medium namely acetate buffer (pH4.0), phosphate buffer (pH7.4) and carbonate buffer (pH10.0).
25063173	13	79	theme	release	1824:1830	arg1	diffusion					1854:1862	Fickian diffusion	1846:1862	Fickian diffusion	1846:1862	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	13	79	theme	release	1824:1830	arg1	mechanism					1832:1840	the main release mechanism	1815:1840	the main release mechanism	1815:1840	Release of asiaticoside from CLA sponges fitted well with first-order kinetic and the exponent value (n) in power law model indicated that the main release mechanism was Fickian diffusion.
25063173	0	80	theme	chitosan	12:19	arg1	sponges					21:27	Hydrophobic chitosan sponges	0:27	Hydrophobic chitosan sponges	0:27	Hydrophobic chitosan sponges modified by aluminum monostearate and dehydrothermal treatment as sustained drug delivery system.
25063173	14	81	theme	further	1954:1960	arg1	application					1962:1972	further application	1954:1972	further application as drug-sustained-release, porous wound dressing	1954:2021	From this study, we found the potential of the prepared hydrophobic chitosan sponges for further application as drug-sustained-release, porous wound dressing.
25063173	2	82	theme	w/v	414:416	arg1	acid					425:428	2% w/v lactic acid	411:428	2% w/v lactic acid	411:428	Alst was able to dissociate and to cleave stearate ion in 2% w/v lactic acid.
25063173	3	83	from	temperature	529:539	arg1	mixing					514:519	simple mixing	507:519	simple mixing at room temperature	507:539	Composite dispersion of chitosan and Alst (CLA) could be easily prepared by simple mixing at room temperature.
25063173	5	84	theme	lyophilization	779:792	arg1	technique					794:802	lyophilization technique	779:802	lyophilization technique	779:802	The dispersions were further fabricated into sponges by using lyophilization technique and DHT.
25063173	1	85	theme	aluminum	288:295	arg1	monostearate					297:308	aluminum monostearate	288:308	aluminum monostearate (Alst)	288:315	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	1	85	theme	aluminum	288:295	arg1	Alst					311:314	Alst	311:314	Alst	311:314	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	8	86	theme	sponges	1076:1082	arg1	behavior					1060:1067	Swelling behavior	1051:1067	Swelling behavior of the sponges	1051:1082	Swelling behavior of the sponges was investigated in three different medium namely acetate buffer (pH4.0), phosphate buffer (pH7.4) and carbonate buffer (pH10.0).
25063173	14	87	theme	sponges	1942:1948	arg1	potential					1895:1903	the potential	1891:1903	the potential of the prepared hydrophobic chitosan sponges for further application as drug-sustained-release, porous wound dressing	1891:2021	From this study, we found the potential of the prepared hydrophobic chitosan sponges for further application as drug-sustained-release, porous wound dressing.
25063173	2	88	theme	2	411:411	arg1	%					412:412	%	412:412	%	412:412	Alst was able to dissociate and to cleave stearate ion in 2% w/v lactic acid.
25063173	0	89	theme	aluminum	41:48	arg1	monostearate					50:61	aluminum monostearate	41:61	aluminum monostearate	41:61	Hydrophobic chitosan sponges modified by aluminum monostearate and dehydrothermal treatment as sustained drug delivery system.
25063173	9	90	theme	model	1308:1312	arg1	asiaticoside					1290:1301	asiaticoside	1290:1301	asiaticoside	1290:1301	Drug release study was conducted in phosphate buffer pH7.4 at 37°C by using asiaticoside as a model drug.
25063173	9	90	theme	model	1308:1312	arg1	drug					1314:1317	a model drug	1306:1317	a model drug	1306:1317	Drug release study was conducted in phosphate buffer pH7.4 at 37°C by using asiaticoside as a model drug.
25063173	14	91	theme	hydrophobic	1921:1931	arg1	sponges					1942:1948	the prepared hydrophobic chitosan sponges	1908:1948	the prepared hydrophobic chitosan sponges	1908:1948	From this study, we found the potential of the prepared hydrophobic chitosan sponges for further application as drug-sustained-release, porous wound dressing.
25063173	12	92	theme	higher	1603:1608	arg1	amount					1615:1620	higher Alst amount	1603:1620	higher Alst amount	1603:1620	Swelling behavior of the sponges was coincident with the release of asiaticoside in which the sponge containing higher Alst amount apparently exhibited the sustained release character.
25063173	6	93	theme	chain	924:928	arg1	group					870:874	amino group	864:874	amino group of chitosan	864:886	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	6	93	theme	chain	924:928	arg1	chain					924:928	stearate side chain	910:928	stearate side chain after DHT	910:938	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	6	93	theme	chain	924:928	arg1	chitosan					879:886	chitosan	879:886	chitosan	879:886	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	6	93	theme	chain	924:928	arg1	group					901:905	carboxyl group	892:905	carboxyl group of stearate side chain after DHT	892:938	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	6	94	theme	spectra	819:825	arg1	analysis					827:834	FT-IR spectra analysis	813:834	FT-IR spectra analysis	813:834	FT-IR spectra analysis indicated amidation between amino group of chitosan and carboxyl group of stearate side chain after DHT.
25063173	3	95	theme	Alst	468:471	arg1	dispersion					441:450	Composite dispersion	431:450	Composite dispersion of chitosan and Alst (CLA)	431:477	Composite dispersion of chitosan and Alst (CLA) could be easily prepared by simple mixing at room temperature.
25063173	1	96	theme	dehydrothermal	321:334	arg1	DHT					347:349	DHT	347:349	DHT	347:349	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	1	96	theme	dehydrothermal	321:334	arg1	treatment					336:344	dehydrothermal treatment	321:344	dehydrothermal treatment (DHT)	321:350	The aim of this study is to develop hydrophobic chitosan sponges by using novel simple preparation technique in which hydrophobicity of chitosan was modified by aluminum monostearate (Alst) and dehydrothermal treatment (DHT).
25063173	10	97	theme	angle	1328:1332	arg1	measurement					1334:1344	Contact angle measurement	1320:1344	Contact angle measurement	1320:1344	Contact angle measurement revealed that addition of Alst and DHT enhanced the hydrophobicity of the materials.
25063173	3	98	theme	chitosan	455:462	arg1	dispersion					441:450	Composite dispersion	431:450	Composite dispersion of chitosan and Alst (CLA)	431:477	Composite dispersion of chitosan and Alst (CLA) could be easily prepared by simple mixing at room temperature.
25063173	8	99	theme	acetate	1134:1140	arg1	buffer					1142:1147	acetate buffer	1134:1147	acetate buffer (pH4.0)	1134:1155	Swelling behavior of the sponges was investigated in three different medium namely acetate buffer (pH4.0), phosphate buffer (pH7.4) and carbonate buffer (pH10.0).
25063173	8	99	theme	acetate	1134:1140	arg1	pH4.0					1150:1154	pH4.0	1150:1154	pH4.0	1150:1154	Swelling behavior of the sponges was investigated in three different medium namely acetate buffer (pH4.0), phosphate buffer (pH7.4) and carbonate buffer (pH10.0).
25063173	4	100	theme	CLA	562:564	arg1	dispersions					566:576	CLA dispersions	562:576	CLA dispersions	562:576	The pH value of the CLA dispersions and particle size of the chitosan-Alst complex in the system comprising low chitosan concentration significantly increased by mixing time.
29030944	0	0	theme	Raman	98:102	arg1	spectroscopy					104:115	surface-enhanced Raman spectroscopy	81:115	surface-enhanced Raman spectroscopy	81:115	Noninvasive detection of nasopharyngeal carcinoma based on saliva proteins using surface-enhanced Raman spectroscopy.
29030944	6	1	theme	Diagnostic	986:995	arg1	sensitivity					997:1007	Diagnostic sensitivity	986:1007	Diagnostic sensitivity of 70.7%	986:1016	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	1	2	theme	analysis	171:178	arg1	capability					150:159	the capability	146:159	the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC)	146:326	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	0	3	theme	surface-enhanced	81:96	arg1	spectroscopy					104:115	surface-enhanced Raman spectroscopy	81:115	surface-enhanced Raman spectroscopy	81:115	Noninvasive detection of nasopharyngeal carcinoma based on saliva proteins using surface-enhanced Raman spectroscopy.
29030944	5	4	theme	healthy	968:974	arg1	subjects					976:983	healthy subjects	968:983	healthy subjects	968:983	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	4	5	theme	Spectral	620:627	arg1	analysis					629:636	Spectral analysis	620:636	Spectral analysis of those saliva protein SERS spectra	620:673	Spectral analysis of those saliva protein SERS spectra revealed specific changes in some biochemical compositions, which were possibly associated with NPC transformation.
29030944	7	6	theme	rapid	1243:1247	arg1	tool					1287:1290	a rapid, noninvasive, and convenient clinical tool	1241:1290	a rapid, noninvasive, and convenient clinical tool for NPC screening	1241:1308	These results show that this assay based on saliva protein SERS analysis holds promising potential for developing a rapid, noninvasive, and convenient clinical tool for NPC screening.
29030944	3	7	with	patients	550:557	arg1	NPC					589:591	pathologically confirmed NPC	564:591	pathologically confirmed NPC	564:591	A total of 659 high-quality SERS spectra were acquired from purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers.
29030944	7	8	theme	noninvasive	1250:1260	arg1	tool					1287:1290	a rapid, noninvasive, and convenient clinical tool	1241:1290	a rapid, noninvasive, and convenient clinical tool for NPC screening	1241:1308	These results show that this assay based on saliva protein SERS analysis holds promising potential for developing a rapid, noninvasive, and convenient clinical tool for NPC screening.
29030944	3	9	theme	healthy	600:606	arg1	volunteers					608:617	71 healthy volunteers	597:617	71 healthy volunteers	597:617	A total of 659 high-quality SERS spectra were acquired from purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers.
29030944	1	10	theme	nasopharyngeal	297:310	arg1	NPC					323:325	NPC	323:325	NPC	323:325	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	1	10	theme	nasopharyngeal	297:310	arg1	carcinoma					312:320	nasopharyngeal carcinoma	297:320	nasopharyngeal carcinoma (NPC)	297:326	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	3	11	theme	volunteers	608:617	arg1	samples					535:541	the saliva samples	524:541	the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers	524:617	A total of 659 high-quality SERS spectra were acquired from purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers.
29030944	2	12	theme	cellulose	389:397	arg1	membrane					407:414	cellulose acetate membrane	389:414	cellulose acetate membrane	389:414	A rapid and convenient protein purification method based on cellulose acetate membrane was developed.
29030944	1	13	theme	carcinoma	312:320	arg1	detection					284:292	noninvasive detection	272:292	noninvasive detection of nasopharyngeal carcinoma (NPC)	272:326	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	5	14	theme	principal	804:812	arg1	analysis					824:831	principal component analysis	804:831	principal component analysis combined with linear discriminant analysis (PCA-LDA)	804:884	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	4	15	theme	NPC	771:773	arg1	transformation					775:788	NPC transformation	771:788	NPC transformation	771:788	Spectral analysis of those saliva protein SERS spectra revealed specific changes in some biochemical compositions, which were possibly associated with NPC transformation.
29030944	5	16	from	NPC	960:962	arg1	spectra					947:953	the saliva protein SERS spectra	923:953	the saliva protein SERS spectra from NPC and healthy subjects	923:983	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	5	17	theme	SERS	942:945	arg1	spectra					947:953	the saliva protein SERS spectra	923:953	the saliva protein SERS spectra from NPC and healthy subjects	923:983	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	1	18	theme	membrane	190:197	arg1	purification					207:218	membrane protein purification	190:218	membrane protein purification	190:218	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	5	19	theme	component	814:822	arg1	analysis					824:831	principal component analysis	804:831	principal component analysis combined with linear discriminant analysis (PCA-LDA)	804:884	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	7	20	theme	SERS	1186:1189	arg1	analysis					1191:1198	saliva protein SERS analysis	1171:1198	saliva protein SERS analysis	1171:1198	These results show that this assay based on saliva protein SERS analysis holds promising potential for developing a rapid, noninvasive, and convenient clinical tool for NPC screening.
29030944	3	21	theme	confirmed	579:587	arg1	NPC					589:591	pathologically confirmed NPC	564:591	pathologically confirmed NPC	564:591	A total of 659 high-quality SERS spectra were acquired from purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers.
29030944	1	22	theme	protein	199:205	arg1	purification					207:218	membrane protein purification	190:218	membrane protein purification	190:218	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	5	23	theme	linear	847:852	arg1	PCA-LDA					877:883	PCA-LDA	877:883	PCA-LDA	877:883	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	5	23	theme	linear	847:852	arg1	analysis					867:874	linear discriminant analysis	847:874	linear discriminant analysis (PCA-LDA)	847:884	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	2	24	theme	convenient	341:350	arg1	method					373:378	A rapid and convenient protein purification method	329:378	A rapid and convenient protein purification method based on cellulose acetate membrane	329:414	A rapid and convenient protein purification method based on cellulose acetate membrane was developed.
29030944	6	25	theme	NPC	1107:1109	arg1	identification					1111:1124	NPC identification	1107:1124	NPC identification	1107:1124	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	3	26	theme	SERS	459:462	arg1	spectra					464:470	659 high-quality SERS spectra	442:470	659 high-quality SERS spectra	442:470	A total of 659 high-quality SERS spectra were acquired from purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers.
29030944	0	27	theme	Noninvasive	0:10	arg1	detection					12:20	Noninvasive detection	0:20	Noninvasive detection of nasopharyngeal carcinoma	0:48	Noninvasive detection of nasopharyngeal carcinoma based on saliva proteins using surface-enhanced Raman spectroscopy.
29030944	6	28	theme	%	1038:1038	arg1	accuracy					1056:1063	diagnostic accuracy	1045:1063	diagnostic accuracy of 70.5%	1045:1072	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	6	28	theme	%	1038:1038	arg1	specificity					1019:1029	specificity	1019:1029	specificity of 70.3%	1019:1038	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	6	28	theme	%	1038:1038	arg1	sensitivity					997:1007	Diagnostic sensitivity	986:1007	Diagnostic sensitivity of 70.7%	986:1016	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	4	29	theme	biochemical	709:719	arg1	compositions					721:732	some biochemical compositions	704:732	some biochemical compositions	704:732	Spectral analysis of those saliva protein SERS spectra revealed specific changes in some biochemical compositions, which were possibly associated with NPC transformation.
29030944	3	30	theme	spectra	464:470	arg1	total					433:437	A total	431:437	A total of 659 high-quality SERS spectra	431:470	A total of 659 high-quality SERS spectra were acquired from purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers.
29030944	0	31	theme	nasopharyngeal	25:38	arg1	carcinoma					40:48	nasopharyngeal carcinoma	25:48	nasopharyngeal carcinoma	25:48	Noninvasive detection of nasopharyngeal carcinoma based on saliva proteins using surface-enhanced Raman spectroscopy.
29030944	5	32	from	subjects	976:983	arg1	spectra					947:953	the saliva protein SERS spectra	923:953	the saliva protein SERS spectra from NPC and healthy subjects	923:983	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	2	33	theme	rapid	331:335	arg1	method					373:378	A rapid and convenient protein purification method	329:378	A rapid and convenient protein purification method based on cellulose acetate membrane	329:414	A rapid and convenient protein purification method based on cellulose acetate membrane was developed.
29030944	4	34	from	changes	693:699	arg1	compositions					721:732	some biochemical compositions	704:732	some biochemical compositions	704:732	Spectral analysis of those saliva protein SERS spectra revealed specific changes in some biochemical compositions, which were possibly associated with NPC transformation.
29030944	1	35	theme	surface-enhanced	225:240	arg1	SERS					262:265	SERS	262:265	SERS	262:265	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	1	35	theme	surface-enhanced	225:240	arg1	spectroscopy					248:259	surface-enhanced Raman spectroscopy	225:259	surface-enhanced Raman spectroscopy (SERS)	225:266	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	7	36	theme	promising	1206:1214	arg1	potential					1216:1224	promising potential	1206:1224	promising potential for developing a rapid, noninvasive, and convenient clinical tool for NPC screening	1206:1308	These results show that this assay based on saliva protein SERS analysis holds promising potential for developing a rapid, noninvasive, and convenient clinical tool for NPC screening.
29030944	1	37	theme	present	122:128	arg1	study					130:134	The present study	118:134	The present study	118:134	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	1	38	theme	Raman	242:246	arg1	SERS					262:265	SERS	262:265	SERS	262:265	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	1	38	theme	Raman	242:246	arg1	spectroscopy					248:259	surface-enhanced Raman spectroscopy	225:259	surface-enhanced Raman spectroscopy (SERS)	225:266	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	5	39	theme	discriminant	854:865	arg1	PCA-LDA					877:883	PCA-LDA	877:883	PCA-LDA	877:883	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	5	39	theme	discriminant	854:865	arg1	analysis					867:874	linear discriminant analysis	847:874	linear discriminant analysis (PCA-LDA)	847:884	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	0	40	theme	carcinoma	40:48	arg1	detection					12:20	Noninvasive detection	0:20	Noninvasive detection of nasopharyngeal carcinoma	0:48	Noninvasive detection of nasopharyngeal carcinoma based on saliva proteins using surface-enhanced Raman spectroscopy.
29030944	3	41	theme	high-quality	446:457	arg1	spectra					464:470	659 high-quality SERS spectra	442:470	659 high-quality SERS spectra	442:470	A total of 659 high-quality SERS spectra were acquired from purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers.
29030944	6	42	theme	diagnostic	1045:1054	arg1	accuracy					1056:1063	diagnostic accuracy	1045:1063	diagnostic accuracy of 70.5%	1045:1072	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	3	43	with	volunteers	608:617	arg1	NPC					589:591	pathologically confirmed NPC	564:591	pathologically confirmed NPC	564:591	A total of 659 high-quality SERS spectra were acquired from purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers.
29030944	3	44	theme	purified	491:498	arg1	proteins					500:507	purified proteins	491:507	purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers	491:617	A total of 659 high-quality SERS spectra were acquired from purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers.
29030944	0	45	theme	saliva	59:64	arg1	proteins					66:73	saliva proteins	59:73	saliva proteins using surface-enhanced Raman spectroscopy	59:115	Noninvasive detection of nasopharyngeal carcinoma based on saliva proteins using surface-enhanced Raman spectroscopy.
29030944	6	46	theme	%	1016:1016	arg1	accuracy					1056:1063	diagnostic accuracy	1045:1063	diagnostic accuracy of 70.5%	1045:1072	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	6	46	theme	%	1016:1016	arg1	specificity					1019:1029	specificity	1019:1029	specificity of 70.3%	1019:1038	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	6	46	theme	%	1016:1016	arg1	sensitivity					997:1007	Diagnostic sensitivity	986:1007	Diagnostic sensitivity of 70.7%	986:1016	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	7	47	theme	convenient	1267:1276	arg1	tool					1287:1290	a rapid, noninvasive, and convenient clinical tool	1241:1290	a rapid, noninvasive, and convenient clinical tool for NPC screening	1241:1308	These results show that this assay based on saliva protein SERS analysis holds promising potential for developing a rapid, noninvasive, and convenient clinical tool for NPC screening.
29030944	3	48	theme	saliva	528:533	arg1	samples					535:541	the saliva samples	524:541	the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers	524:617	A total of 659 high-quality SERS spectra were acquired from purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers.
29030944	7	49	theme	protein	1178:1184	arg1	analysis					1191:1198	saliva protein SERS analysis	1171:1198	saliva protein SERS analysis	1171:1198	These results show that this assay based on saliva protein SERS analysis holds promising potential for developing a rapid, noninvasive, and convenient clinical tool for NPC screening.
29030944	5	50	used	utilized	890:897	arg2	analysis					824:831	principal component analysis	804:831	principal component analysis combined with linear discriminant analysis (PCA-LDA)	804:884	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	1	51	theme	saliva	164:169	arg1	analysis					171:178	saliva analysis	164:178	saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS)	164:266	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
29030944	7	52	theme	NPC	1296:1298	arg1	screening					1300:1308	NPC screening	1296:1308	NPC screening	1296:1308	These results show that this assay based on saliva protein SERS analysis holds promising potential for developing a rapid, noninvasive, and convenient clinical tool for NPC screening.
29030944	6	53	theme	%	1072:1072	arg1	accuracy					1056:1063	diagnostic accuracy	1045:1063	diagnostic accuracy of 70.5%	1045:1072	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	6	53	theme	%	1072:1072	arg1	specificity					1019:1029	specificity	1019:1029	specificity of 70.3%	1019:1038	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	6	53	theme	%	1072:1072	arg1	sensitivity					997:1007	Diagnostic sensitivity	986:1007	Diagnostic sensitivity of 70.7%	986:1016	Diagnostic sensitivity of 70.7%, specificity of 70.3%, and diagnostic accuracy of 70.5% could be achieved by PCA-LDA for NPC identification.
29030944	2	54	theme	purification	360:371	arg1	method					373:378	A rapid and convenient protein purification method	329:378	A rapid and convenient protein purification method based on cellulose acetate membrane	329:414	A rapid and convenient protein purification method based on cellulose acetate membrane was developed.
29030944	4	55	theme	protein	654:660	arg1	spectra					667:673	those saliva protein SERS spectra	641:673	those saliva protein SERS spectra	641:673	Spectral analysis of those saliva protein SERS spectra revealed specific changes in some biochemical compositions, which were possibly associated with NPC transformation.
29030944	5	56	theme	saliva	927:932	arg1	spectra					947:953	the saliva protein SERS spectra	923:953	the saliva protein SERS spectra from NPC and healthy subjects	923:983	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	2	57	theme	protein	352:358	arg1	method					373:378	A rapid and convenient protein purification method	329:378	A rapid and convenient protein purification method based on cellulose acetate membrane	329:414	A rapid and convenient protein purification method based on cellulose acetate membrane was developed.
29030944	4	58	theme	saliva	647:652	arg1	spectra					667:673	those saliva protein SERS spectra	641:673	those saliva protein SERS spectra	641:673	Spectral analysis of those saliva protein SERS spectra revealed specific changes in some biochemical compositions, which were possibly associated with NPC transformation.
29030944	4	59	theme	specific	684:691	arg1	changes					693:699	specific changes	684:699	specific changes	684:699	Spectral analysis of those saliva protein SERS spectra revealed specific changes in some biochemical compositions, which were possibly associated with NPC transformation.
29030944	3	60	theme	patients	550:557	arg1	samples					535:541	the saliva samples	524:541	the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers	524:617	A total of 659 high-quality SERS spectra were acquired from purified proteins extracted from the saliva samples of 170 patients with pathologically confirmed NPC and 71 healthy volunteers.
29030944	5	61	theme	protein	934:940	arg1	spectra					947:953	the saliva protein SERS spectra	923:953	the saliva protein SERS spectra from NPC and healthy subjects	923:983	Furthermore, principal component analysis combined with linear discriminant analysis (PCA-LDA) was utilized to analyze and classify the saliva protein SERS spectra from NPC and healthy subjects.
29030944	4	62	theme	spectra	667:673	arg1	analysis					629:636	Spectral analysis	620:636	Spectral analysis of those saliva protein SERS spectra	620:673	Spectral analysis of those saliva protein SERS spectra revealed specific changes in some biochemical compositions, which were possibly associated with NPC transformation.
29030944	7	63	theme	saliva	1171:1176	arg1	analysis					1191:1198	saliva protein SERS analysis	1171:1198	saliva protein SERS analysis	1171:1198	These results show that this assay based on saliva protein SERS analysis holds promising potential for developing a rapid, noninvasive, and convenient clinical tool for NPC screening.
29030944	4	64	theme	SERS	662:665	arg1	spectra					667:673	those saliva protein SERS spectra	641:673	those saliva protein SERS spectra	641:673	Spectral analysis of those saliva protein SERS spectra revealed specific changes in some biochemical compositions, which were possibly associated with NPC transformation.
29030944	2	65	theme	acetate	399:405	arg1	membrane					407:414	cellulose acetate membrane	389:414	cellulose acetate membrane	389:414	A rapid and convenient protein purification method based on cellulose acetate membrane was developed.
29030944	7	66	theme	clinical	1278:1285	arg1	tool					1287:1290	a rapid, noninvasive, and convenient clinical tool	1241:1290	a rapid, noninvasive, and convenient clinical tool for NPC screening	1241:1308	These results show that this assay based on saliva protein SERS analysis holds promising potential for developing a rapid, noninvasive, and convenient clinical tool for NPC screening.
29030944	1	67	theme	noninvasive	272:282	arg1	detection					284:292	noninvasive detection	272:292	noninvasive detection of nasopharyngeal carcinoma (NPC)	272:326	The present study evaluated the capability of saliva analysis combining membrane protein purification with surface-enhanced Raman spectroscopy (SERS) for noninvasive detection of nasopharyngeal carcinoma (NPC).
24832112	9	0	dep	CONCLUSIONS	1896:1906	arg1	offer					2039:2043	offer	2039:2043	may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs	2030:2131	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	9	0	dep	CONCLUSIONS	1896:1906	arg1	provide					1936:1942	provide	1936:1942	provide more insight into better understanding of the pharmacological mechanisms of FXST	1936:2023	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	6	1	dep	Chen	1365:1368	arg1	adjuvant					1388:1395	adjuvant	1388:1395	adjuvant	1388:1395	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	7	2	theme	pharmacology	1486:1497	arg1	analysis					1510:1517	our network pharmacology (NetPharm) analysis	1474:1517	our network pharmacology (NetPharm) analysis	1474:1517	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	9	3	theme	pharmacological	1990:2004	arg1	mechanisms					2006:2015	the pharmacological mechanisms	1986:2015	the pharmacological mechanisms of FXST	1986:2023	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	3	4	theme	effects	588:594	arg1	complexity					537:546	its complexity	533:546	its complexity of compositions and polypharmacological effects	533:594	MATERIALS AND METHODS Due to its complexity of compositions and polypharmacological effects, it often complicates understanding of the mechanisms of action.
24832112	8	5	with	congruent	1831:1839	arg1	prediction					1863:1872	the cross-target prediction	1846:1872	the cross-target prediction by NetPharm approach	1846:1893	The results showed that FXST could significantly ameliorate the activation of coagulation system, which is congruent with the cross-target prediction by NetPharm approach.
24832112	6	6	theme	Jun	1349:1351	arg1	"					-1:-1		-1:-1		-1:-1	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	8	7	theme	coagulation	1802:1812	arg1	system					1814:1819	coagulation system	1802:1819	coagulation system	1802:1819	The results showed that FXST could significantly ameliorate the activation of coagulation system, which is congruent with the cross-target prediction by NetPharm approach.
24832112	7	8	theme	rat	1713:1715	arg1	model					1717:1721	a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model	1639:1721	a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model	1639:1721	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	7	9	theme	polypharmacological	1433:1451	arg1	effects					1453:1459	the polypharmacological effects	1429:1459	the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis	1429:1517	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	0	10	theme	disseminated	141:152	arg1	coagulation					168:178	disseminated intravascular coagulation rats	141:183	disseminated intravascular coagulation rats	141:183	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	6	11	theme	"	-1:-1	arg1	emperor					1354:1360	emperor	1354:1360	emperor	1354:1360	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	6	11	theme	"	-1:-1	arg1	Jun					1349:1351		-1:1351		-1:1361	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	6	11	theme	"	-1:-1	arg1	courier					1405:1411	courier	1405:1411	courier	1405:1411	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	3	12	theme	action	653:658	arg1	mechanisms					639:648	the mechanisms	635:648	the mechanisms of action	635:658	MATERIALS AND METHODS Due to its complexity of compositions and polypharmacological effects, it often complicates understanding of the mechanisms of action.
24832112	7	13	theme	coagulation	1695:1705	arg1	model					1717:1721	a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model	1639:1721	a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model	1639:1721	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	9	14	theme	alternative	2048:2058	arg1	avenue					2060:2065	an alternative avenue	2045:2065	an alternative avenue to further explore the chemical and pharmacological basis of TCMs	2045:2131	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	3	15	theme	mechanisms	639:648	arg1	understanding					618:630	understanding	618:630	understanding of the mechanisms of action	618:658	MATERIALS AND METHODS Due to its complexity of compositions and polypharmacological effects, it often complicates understanding of the mechanisms of action.
24832112	5	16	from	ingredients	897:907	arg1	FXST					912:915	FXST	912:915	FXST	912:915	RESULTS The predicted results showed that 22 ingredients in FXST were closely associated with 41 protein targets related to blood coagulation, fibrinolysis and platelet aggregation.
24832112	7	17	theme	disseminated	1668:1679	arg1	coagulation					1695:1705	disseminated intravascular coagulation	1668:1705	a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model	1639:1721	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	8	18	theme	cross-target	1850:1861	arg1	prediction					1863:1872	the cross-target prediction	1846:1872	the cross-target prediction by NetPharm approach	1846:1893	The results showed that FXST could significantly ameliorate the activation of coagulation system, which is congruent with the cross-target prediction by NetPharm approach.
24832112	6	19	theme	Chinese	1319:1325	arg1	medicines					1327:1335	Traditional Chinese medicines	1307:1335	Traditional Chinese medicines (TCMs)	1307:1342	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	6	19	theme	Chinese	1319:1325	arg1	TCMs					1338:1341	TCMs	1338:1341	TCMs	1338:1341	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	4	20	theme	system	725:730	arg1	pharmacology					732:743	system pharmacology	725:743	system pharmacology	725:743	In the present work, we have constructed an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease.
24832112	0	21	dep	coagulation	168:178	arg1	rats					180:183	rats	180:183	disseminated intravascular coagulation rats	141:183	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	7	22	theme	system	1629:1634	arg1	disorders					1594:1602	the disorders	1590:1602	the disorders of the blood coagulation system	1590:1634	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	9	23	theme	chemical	2090:2097	arg1	basis					2119:2123	the chemical and pharmacological basis	2086:2123	the chemical and pharmacological basis of TCMs	2086:2131	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	0	24	theme	Xueshuantong	88:99	arg1	Capsule					101:107	Fufang Xueshuantong Capsule	81:107	Fufang Xueshuantong Capsule	81:107	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	1	25	theme	miltiorrhizae	378:390	arg1	formula					324:330	a four-herb formula	312:330	a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae	312:417	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	1	26	theme	Fufang	217:222	arg1	Capsule					244:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule	186:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule	186:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	6	27	theme	association	1082:1092	arg1	analysis					1042:1049	analysis	1042:1049	analysis of the compound-protein target association	1042:1092	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	9	28	theme	pharmacological	2103:2117	arg1	basis					2119:2123	the chemical and pharmacological basis	2086:2123	the chemical and pharmacological basis of TCMs	2086:2131	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	6	29	dep	indicated	1139:1147	arg1	interact					1196:1203	interact	1196:1203		1139:-1	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	1	30	theme	Radix	396:400	arg1	scrophulariaceae					402:417	Radix scrophulariaceae	396:417	Radix scrophulariaceae	396:417	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	6	31	theme	principle	1294:1302	arg1	mechanisms					1276:1285	the synergistic mechanisms	1260:1285		1260:-1	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	6	32	theme	compound-protein	1058:1073	arg1	association					1082:1092	the compound-protein target association	1054:1092	the compound-protein target association	1054:1092	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	4	33	theme	disease	843:849	arg1	treatment					819:827	treatment	819:827	treatment of thrombosis disease	819:849	In the present work, we have constructed an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease.
24832112	2	34	theme	many	492:495	arg1	years					497:501	many years	492:501	many years	492:501	It has been used for treatment of the clinic cardiovascular disease for many years.
24832112	4	35	theme	FXST	798:801	arg1	formulation					803:813	FXST formulation	798:813	FXST formulation	798:813	In the present work, we have constructed an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease.
24832112	0	36	theme	Network	0:6	arg1	analyses					21:28	Network pharmacology analyses	0:28	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.	0:184	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	1	37	theme	four-herb	314:322	arg1	formula					324:330	a four-herb formula	312:330	a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae	312:417	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	5	38	theme	platelet	1012:1019	arg1	aggregation					1021:1031	platelet aggregation	1012:1031	platelet aggregation	1012:1031	RESULTS The predicted results showed that 22 ingredients in FXST were closely associated with 41 protein targets related to blood coagulation, fibrinolysis and platelet aggregation.
24832112	6	39	theme	active	1162:1167	arg1	ingredients					1178:1188	the multiple active chemical ingredients	1149:1188	the multiple active chemical ingredients	1149:1188	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	2	40	theme	disease	480:486	arg1	treatment					441:449	treatment	441:449	treatment of the clinic cardiovascular disease for many years	441:501	It has been used for treatment of the clinic cardiovascular disease for many years.
24832112	2	41	theme	clinic	458:463	arg1	disease					480:486	the clinic cardiovascular disease	454:486	the clinic cardiovascular disease	454:486	It has been used for treatment of the clinic cardiovascular disease for many years.
24832112	7	42	dep	carried	1528:1534	arg1	out					1536:1538	out	1536:1538	out	1536:1538	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	1	43	theme	notoginseng	341:351	arg1	formula					324:330	a four-herb formula	312:330	a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae	312:417	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	0	44	with	analyses	21:28	arg1	support					127:133	experimental support	114:133	experimental support using disseminated intravascular coagulation rats	114:183	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	5	45	theme	predicted	864:872	arg1	results					874:880	The predicted results	860:880	The predicted results	860:880	RESULTS The predicted results showed that 22 ingredients in FXST were closely associated with 41 protein targets related to blood coagulation, fibrinolysis and platelet aggregation.
24832112	1	46	theme	Radix	354:358	arg1	astragali					360:368	Radix astragali	354:368	Radix astragali	354:368	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	9	47	theme	more	1944:1947	arg1	insight					1949:1955	more insight	1944:1955	more insight into better understanding of the pharmacological mechanisms of FXST	1944:2023	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	7	48	theme	experimental	1540:1551	arg1	investigation					1553:1565	experimental investigation	1540:1565	experimental investigation	1540:1565	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	1	49	theme	ETHNOPHARMACOLOGICAL	186:205	arg1	Capsule					244:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule	186:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule	186:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	3	50	theme	compositions	551:562	arg1	complexity					537:546	its complexity	533:546	its complexity of compositions and polypharmacological effects	533:594	MATERIALS AND METHODS Due to its complexity of compositions and polypharmacological effects, it often complicates understanding of the mechanisms of action.
24832112	1	51	theme	Chinese	282:288	arg1	medicine					290:297	a traditional Chinese medicine	268:297	a traditional Chinese medicine remedy	268:304	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	6	52	theme	significant	1095:1105	arg1	cross-targets					1107:1119	significant cross-targets	1095:1119	significant cross-targets between each herb	1095:1137	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	7	53	from	effects	1571:1577	arg1	disorders					1594:1602	the disorders	1590:1602	the disorders of the blood coagulation system	1590:1634	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	6	54	theme	Chen	1365:1368	arg1	"					-1:-1		-1:-1		-1:-1	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	5	55	theme	related	965:971	arg1	targets					957:963	41 protein targets	946:963	41 protein targets related to blood coagulation, fibrinolysis and platelet aggregation	946:1031	RESULTS The predicted results showed that 22 ingredients in FXST were closely associated with 41 protein targets related to blood coagulation, fibrinolysis and platelet aggregation.
24832112	9	56	theme	mechanisms	2006:2015	arg1	understanding					1969:1981	better understanding	1962:1981	better understanding of the pharmacological mechanisms of FXST	1962:2023	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	3	57	theme	polypharmacological	568:586	arg1	effects					588:594	polypharmacological effects	568:594	polypharmacological effects	568:594	MATERIALS AND METHODS Due to its complexity of compositions and polypharmacological effects, it often complicates understanding of the mechanisms of action.
24832112	1	58	theme	Xueshuantong	224:235	arg1	Capsule					244:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule	186:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule	186:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	0	59	theme	pharmacological	52:66	arg1	mechanism					68:76	the antithrombotic pharmacological mechanism	33:76	the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule	33:107	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	7	60	theme	NetPharm	1500:1507	arg1	analysis					1510:1517	our network pharmacology (NetPharm) analysis	1474:1517	our network pharmacology (NetPharm) analysis	1474:1517	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	7	61	theme	network	1478:1484	arg1	analysis					1510:1517	our network pharmacology (NetPharm) analysis	1474:1517	our network pharmacology (NetPharm) analysis	1474:1517	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	9	62	theme	FXST	2020:2023	arg1	mechanisms					2006:2015	the pharmacological mechanisms	1986:2015	the pharmacological mechanisms of FXST	1986:2023	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	1	63	theme	FXST	238:241	arg1	Capsule					244:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule	186:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule	186:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	5	64	dep	RESULTS	852:858	arg1	showed					882:887	showed	882:887	showed that 22 ingredients in FXST were closely associated with 41 protein targets related to blood coagulation, fibrinolysis and platelet aggregation	882:1031	RESULTS The predicted results showed that 22 ingredients in FXST were closely associated with 41 protein targets related to blood coagulation, fibrinolysis and platelet aggregation.
24832112	6	65	theme	synergistic	1264:1274	arg1	mechanisms					1276:1285	the synergistic mechanisms	1260:1285		1260:-1	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	4	66	theme	integrated	705:714	arg1	model					716:720	an integrated model	702:720	an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease	702:849	In the present work, we have constructed an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease.
24832112	0	67	theme	intravascular	154:166	arg1	coagulation					168:178	disseminated intravascular coagulation rats	141:183	disseminated intravascular coagulation rats	141:183	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	8	68	theme	system	1814:1819	arg1	congruent					1831:1839	congruent	1831:1839	congruent	1831:1839	The results showed that FXST could significantly ameliorate the activation of coagulation system, which is congruent with the cross-target prediction by NetPharm approach.
24832112	8	68	theme	system	1814:1819	arg1	activation					1788:1797	the activation	1784:1797	the activation	1784:1797	The results showed that FXST could significantly ameliorate the activation of coagulation system, which is congruent with the cross-target prediction by NetPharm approach.
24832112	7	69	theme	intravascular	1681:1693	arg1	coagulation					1695:1705	disseminated intravascular coagulation	1668:1705	a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model	1639:1721	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	7	70	theme	blood	1611:1615	arg1	system					1629:1634	the blood coagulation system	1607:1634	the blood coagulation system	1607:1634	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	5	71	theme	blood	976:980	arg1	coagulation					982:992	blood coagulation	976:992	blood coagulation	976:992	RESULTS The predicted results showed that 22 ingredients in FXST were closely associated with 41 protein targets related to blood coagulation, fibrinolysis and platelet aggregation.
24832112	7	72	theme	lipopolysaccharide-induced	1641:1666	arg1	model					1717:1721	a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model	1639:1721	a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model	1639:1721	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	6	73	theme	medicines	1327:1335	arg1	principle					1294:1302	the principle	1290:1302	the principle of Traditional Chinese medicines (TCMs)	1290:1342	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	4	74	theme	polypharmacological	764:782	arg1	mechanisms					784:793	the polypharmacological mechanisms	760:793	the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease	760:849	In the present work, we have constructed an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease.
24832112	0	75	theme	Fufang	81:86	arg1	Capsule					101:107	Fufang Xueshuantong Capsule	81:107	Fufang Xueshuantong Capsule	81:107	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	5	76	theme	protein	949:955	arg1	targets					957:963	41 protein targets	946:963	41 protein targets related to blood coagulation, fibrinolysis and platelet aggregation	946:1031	RESULTS The predicted results showed that 22 ingredients in FXST were closely associated with 41 protein targets related to blood coagulation, fibrinolysis and platelet aggregation.
24832112	7	77	theme	coagulation	1617:1627	arg1	system					1629:1634	the blood coagulation system	1607:1634	the blood coagulation system	1607:1634	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	6	78	theme	Traditional	1307:1317	arg1	medicines					1327:1335	Traditional Chinese medicines	1307:1335	Traditional Chinese medicines (TCMs)	1307:1342	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	6	78	theme	Traditional	1307:1317	arg1	TCMs					1338:1341	TCMs	1338:1341	TCMs	1338:1341	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	2	79	used	used	432:435	arg2	It					420:421	It	420:421	It	420:421	It has been used for treatment of the clinic cardiovascular disease for many years.
24832112	0	80	theme	Capsule	101:107	arg1	mechanism					68:76	the antithrombotic pharmacological mechanism	33:76	the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule	33:107	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	1	81	theme	Salvia	371:376	arg1	miltiorrhizae					378:390	Salvia miltiorrhizae	371:390	Salvia miltiorrhizae	371:390	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	1	82	theme	RELEVANCE	207:215	arg1	Capsule					244:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule	186:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule	186:250	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	4	83	theme	pharmacology	732:743	arg1	model					716:720	an integrated model	702:720	an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease	702:849	In the present work, we have constructed an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease.
24832112	0	84	theme	experimental	114:125	arg1	support					127:133	experimental support	114:133	experimental support using disseminated intravascular coagulation rats	114:183	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	8	85	theme	NetPharm	1877:1884	arg1	approach					1886:1893	NetPharm approach	1877:1893	NetPharm approach	1877:1893	The results showed that FXST could significantly ameliorate the activation of coagulation system, which is congruent with the cross-target prediction by NetPharm approach.
24832112	6	86	theme	target	1075:1080	arg1	association					1082:1092	the compound-protein target association	1054:1092	the compound-protein target association	1054:1092	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	1	87	theme	scrophulariaceae	402:417	arg1	formula					324:330	a four-herb formula	312:330	a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae	312:417	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	9	88	theme	TCMs	2128:2131	arg1	basis					2119:2123	the chemical and pharmacological basis	2086:2123	the chemical and pharmacological basis of TCMs	2086:2131	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	4	89	theme	thrombosis	832:841	arg1	disease					843:849	thrombosis disease	832:849	thrombosis disease	832:849	In the present work, we have constructed an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease.
24832112	0	90	theme	pharmacology	8:19	arg1	analyses					21:28	Network pharmacology analyses	0:28	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.	0:184	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	7	91	dep	coagulation	1695:1705	arg1	DIC					1708:1710	DIC	1708:1710	DIC	1708:1710	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	6	92	theme	chemical	1169:1176	arg1	ingredients					1178:1188	the multiple active chemical ingredients	1149:1188	the multiple active chemical ingredients	1149:1188	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	6	93	theme	multiple	1153:1160	arg1	ingredients					1178:1188	the multiple active chemical ingredients	1149:1188	the multiple active chemical ingredients	1149:1188	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	2	94	theme	cardiovascular	465:478	arg1	disease					480:486	the clinic cardiovascular disease	454:486	the clinic cardiovascular disease	454:486	It has been used for treatment of the clinic cardiovascular disease for many years.
24832112	4	95	theme	formulation	803:813	arg1	mechanisms					784:793	the polypharmacological mechanisms	760:793	the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease	760:849	In the present work, we have constructed an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease.
24832112	0	96	theme	antithrombotic	37:50	arg1	mechanism					68:76	the antithrombotic pharmacological mechanism	33:76	the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule	33:107	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	1	97	theme	Panax	335:339	arg1	notoginseng					341:351	Panax notoginseng	335:351	Panax notoginseng	335:351	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	9	98	theme	combined	1912:1919	arg1	investigations					1921:1934	The combined investigations	1908:1934	The combined investigations	1908:1934	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	0	99	theme	mechanism	68:76	arg1	analyses					21:28	Network pharmacology analyses	0:28	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.	0:184	Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats.
24832112	7	100	theme	FXST	1582:1585	arg1	effects					1571:1577	the effects	1567:1577	the effects of FXST on the disorders of the blood coagulation system	1567:1634	To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model.
24832112	1	101	theme	astragali	360:368	arg1	formula					324:330	a four-herb formula	312:330	a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae	312:417	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	3	102	theme	Due	526:528	arg1	MATERIALS					504:512	MATERIALS	504:512	MATERIALS	504:512	MATERIALS AND METHODS Due to its complexity of compositions and polypharmacological effects, it often complicates understanding of the mechanisms of action.
24832112	1	103	theme	traditional	270:280	arg1	medicine					290:297	a traditional Chinese medicine	268:297	a traditional Chinese medicine remedy	268:304	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24832112	6	104	theme	same	1214:1217	arg1	target					1219:1224	the same target	1210:1224	the same target	1210:1224	Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as ''Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)''.
24832112	9	105	theme	better	1962:1967	arg1	understanding					1969:1981	better understanding	1962:1981	better understanding of the pharmacological mechanisms of FXST	1962:2023	CONCLUSIONS The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs.
24832112	4	106	theme	present	668:674	arg1	work					676:679	the present work	664:679	the present work	664:679	In the present work, we have constructed an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease.
24832112	1	107	theme	medicine	290:297	arg1	remedy					299:304	a traditional Chinese medicine remedy	268:304	a traditional Chinese medicine remedy	268:304	ETHNOPHARMACOLOGICAL RELEVANCE Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae.
24504696	6	0	dep	components	1214:1223	arg1	components					1214:1223	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	0	dep	components	1214:1223	arg1	IRX10					1234:1238	IRX10	1234:1238	IRX10	1234:1238	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	0	dep	components	1214:1223	arg1	IRX9					1225:1228	IRX9	1225:1228	IRX9	1225:1228	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	1	theme	synthase	1205:1212	arg1	components					1214:1223	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	1	theme	synthase	1205:1212	arg1	IRX10					1234:1238	IRX10	1234:1238	IRX10	1234:1238	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	1	theme	synthase	1205:1212	arg1	IRX9					1225:1228	IRX9	1225:1228	IRX9	1225:1228	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	1	theme	synthase	1205:1212	arg1	GUX1					1273:1276	xylan glucuronyl transferase GUX1	1244:1276	xylan glucuronyl transferase GUX1	1244:1276	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	2	theme	parenchyma	1487:1496	arg1	cells					1498:1502	ray parenchyma cells	1483:1502	ray parenchyma cells	1483:1502	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	5	3	theme	fibre	1144:1148	arg1	cells					1150:1154	phloem fibre cells	1137:1154	phloem fibre cells	1137:1154	Histochemical staining and immunolabeling revealed that increased deposition of lignin and xylan was associated with xylem, xylem fibre cells and phloem fibre cells.
24504696	4	4	theme	big	784:786	arg1	increase					788:795	a big increase	782:795	a big increase in xylan content	782:812	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	7	5	from	information	1644:1654	arg1	relationship					1663:1674	the relationship	1659:1674	the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition	1659:1764	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	6	6	theme	encoding	1190:1197	arg1	components					1214:1223	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	6	theme	encoding	1190:1197	arg1	IRX10					1234:1238	IRX10	1234:1238	IRX10	1234:1238	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	6	theme	encoding	1190:1197	arg1	IRX9					1225:1228	IRX9	1225:1228	IRX9	1225:1228	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	6	theme	encoding	1190:1197	arg1	GUX1					1273:1276	xylan glucuronyl transferase GUX1	1244:1276	xylan glucuronyl transferase GUX1	1244:1276	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	3	7	theme	culture-derived	522:536	arg1	system					538:543	a tractable bud culture-derived system	506:543	a tractable bud culture-derived system	506:543	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	7	8	theme	temporal	1696:1703	arg1	variation					1705:1713	spatial and temporal variation	1684:1713	spatial and temporal variation in xylan-related transcripts	1684:1742	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	1	9	from	willow	158:163	arg1	properties					111:120	The properties	107:120	The properties of the secondary cell wall (SCW) in willow	107:163	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	3	10	theme	tractable	508:516	arg1	system					538:543	a tractable bud culture-derived system	506:543	a tractable bud culture-derived system	506:543	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	7	11	theme	close	1566:1570	arg1	similarity					1572:1581	a close similarity	1564:1581	a close similarity with SCW development in Populus species	1564:1621	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	7	12	dep	variation	1705:1713	arg1	composition					1754:1764	composition	1754:1764	composition	1754:1764	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	6	13	theme	orthologous	1169:1179	arg1	Transcripts					1157:1167	Transcripts	1157:1167	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1157:1291	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	14	theme	transferase	1261:1271	arg1	GUX1					1273:1276	xylan glucuronyl transferase GUX1	1244:1276	xylan glucuronyl transferase GUX1	1244:1276	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	14	theme	transferase	1261:1271	arg1	components					1214:1223	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	15	theme	xylan	1244:1248	arg1	GUX1					1273:1276	xylan glucuronyl transferase GUX1	1244:1276	xylan glucuronyl transferase GUX1	1244:1276	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	15	theme	xylan	1244:1248	arg1	components					1214:1223	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	16	theme	phloem	1527:1532	arg1	cells					1540:1544	phloem fibre cells	1527:1544	phloem fibre cells	1527:1544	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	5	17	theme	phloem	1137:1142	arg1	cells					1150:1154	phloem fibre cells	1137:1154	phloem fibre cells	1137:1154	Histochemical staining and immunolabeling revealed that increased deposition of lignin and xylan was associated with xylem, xylem fibre cells and phloem fibre cells.
24504696	4	18	theme	large	681:685	arg1	increase					687:694	A large increase	679:694	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil	679:759	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	4	19	from	change	822:827	arg1	frequency					836:844	the frequency	832:844	the frequency of substitution of xylan with glucuronic acid	832:890	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	6	20	theme	same	1327:1330	arg1	pattern					1340:1346	the same spatial pattern	1323:1346	the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells	1323:1544	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	1	21	theme	secondary	129:137	arg1	SCW					150:152	SCW	150:152	SCW	150:152	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	1	21	theme	secondary	129:137	arg1	wall					144:147	the secondary cell wall	125:147	the secondary cell wall (SCW) in willow	125:163	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	0	22	theme	willow	76:81	arg1	stems					100:104	developing willow (Salix purpurea) stems	65:104	developing willow (Salix purpurea) stems	65:104	Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems.
24504696	5	23	theme	lignin	1071:1076	arg1	deposition					1057:1066	increased deposition	1047:1066	increased deposition of lignin and xylan	1047:1086	Histochemical staining and immunolabeling revealed that increased deposition of lignin and xylan was associated with xylem, xylem fibre cells and phloem fibre cells.
24504696	1	24	theme	wall	144:147	arg1	properties					111:120	The properties	107:120	The properties of the secondary cell wall (SCW) in willow	107:163	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	0	25	theme	Salix	84:88	arg1	stems					100:104	developing willow (Salix purpurea) stems	65:104	developing willow (Salix purpurea) stems	65:104	Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems.
24504696	4	26	theme	0-2	728:730	arg1	weeks					732:736	the period 0-2 weeks	717:736	the period 0-2 weeks after transfer to soil	717:759	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	1	27	theme	bioenergy	241:249	arg1	applications					263:274	potential bioenergy and biofuel applications	231:274	potential bioenergy and biofuel applications	231:274	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	1	28	from	properties	111:120	arg1	willow					158:163	willow	158:163	willow	158:163	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	0	29	theme	developing	65:74	arg1	stems					100:104	developing willow (Salix purpurea) stems	65:104	developing willow (Salix purpurea) stems	65:104	Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems.
24504696	0	30	theme	Secondary	0:8	arg1	composition					20:30	Secondary cell wall composition	0:30	Secondary cell wall composition	0:30	Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems.
24504696	6	31	from	GUX1	1273:1276	arg1	Arabidopsis					1281:1291	Arabidopsis	1281:1291	Arabidopsis	1281:1291	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	7	32	with	similarity	1572:1581	arg1	development					1592:1602	SCW development	1588:1602	SCW development	1588:1602	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	4	33	theme	cellulose	962:970	arg1	synthesis					945:953	synthesis	945:953	synthesis of SCW cellulose, xylan and lignin	945:988	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	0	34	theme	wall	15:18	arg1	composition					20:30	Secondary cell wall composition	0:30	Secondary cell wall composition	0:30	Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems.
24504696	5	35	theme	xylem	1115:1119	arg1	cells					1127:1131	xylem fibre cells	1115:1131	xylem fibre cells	1115:1131	Histochemical staining and immunolabeling revealed that increased deposition of lignin and xylan was associated with xylem, xylem fibre cells and phloem fibre cells.
24504696	7	36	theme	SCW	1588:1590	arg1	development					1592:1602	SCW development	1588:1602	SCW development	1588:1602	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	4	37	theme	putative	920:927	arg1	transcripts					929:939	putative transcripts	920:939	putative transcripts for synthesis of SCW cellulose, xylan and lignin	920:988	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	2	38	theme	model	372:376	arg1	Populus					426:432	the closely related genus Populus	400:432	the closely related genus Populus	400:432	SCW development has been little studied in willow and it is not known how willow compares with model species, particularly the closely related genus Populus.
24504696	2	38	theme	model	372:376	arg1	species					378:384	model species	372:384	model species	372:384	SCW development has been little studied in willow and it is not known how willow compares with model species, particularly the closely related genus Populus.
24504696	6	39	theme	expression	1351:1360	arg1	pattern					1340:1346	the same spatial pattern	1323:1346	the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells	1323:1544	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	5	40	theme	xylan	1082:1086	arg1	deposition					1057:1066	increased deposition	1047:1066	increased deposition of lignin and xylan	1047:1086	Histochemical staining and immunolabeling revealed that increased deposition of lignin and xylan was associated with xylem, xylem fibre cells and phloem fibre cells.
24504696	3	41	theme	wall	587:590	arg1	composition					592:602	cell wall composition	582:602	cell wall composition	582:602	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	0	42	theme	gene	46:49	arg1	expression					51:60	candidate gene expression	36:60	candidate gene expression	36:60	Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems.
24504696	6	43	theme	abundant	1431:1438	arg1	expression					1440:1449	abundant expression	1431:1449	abundant expression in proto-xylem, xylem fibre and ray parenchyma cells	1431:1502	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	1	44	theme	willow	202:207	arg1	feedstock					217:225	willow biomass feedstock	202:225	willow biomass feedstock	202:225	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	4	45	theme	xylan	973:977	arg1	synthesis					945:953	synthesis	945:953	synthesis of SCW cellulose, xylan and lignin	945:988	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	3	46	theme	early	660:664	arg1	development					666:676	early development	660:676	early development	660:676	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	3	47	theme	SCW	462:464	arg1	synthesis					466:474	SCW synthesis	462:474	SCW synthesis	462:474	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	6	48	theme	developmental	1404:1416	arg1	stages					1418:1423	four developmental stages	1399:1423	four developmental stages	1399:1423	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	1	49	theme	feedstock	217:225	arg1	suitability					187:197	the suitability	183:197	the suitability of willow biomass feedstock for potential bioenergy and biofuel applications	183:274	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	4	50	theme	xylan	800:804	arg1	content					806:812	xylan content	800:812	xylan content	800:812	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	7	51	from	xylan	1748:1752	arg1	transcripts					1732:1742	xylan-related transcripts	1718:1742	xylan-related transcripts	1718:1742	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	4	52	theme	lignin	983:988	arg1	synthesis					945:953	synthesis	945:953	synthesis of SCW cellulose, xylan and lignin	945:988	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	1	53	theme	potential	231:239	arg1	applications					263:274	potential bioenergy and biofuel applications	231:274	potential bioenergy and biofuel applications	231:274	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	7	54	theme	xylan-related	1718:1730	arg1	transcripts					1732:1742	xylan-related transcripts	1718:1742	xylan-related transcripts	1718:1742	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	6	55	from	expression	1513:1522	arg1	cells					1498:1502	ray parenchyma cells	1483:1502	ray parenchyma cells	1483:1502	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	55	from	expression	1513:1522	arg1	xylem					1467:1471	xylem fibre	1467:1477	xylem fibre	1467:1477	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	55	from	expression	1513:1522	arg1	proto-xylem					1454:1464	proto-xylem	1454:1464	proto-xylem	1454:1464	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	55	from	expression	1513:1522	arg1	cells					1540:1544	phloem fibre cells	1527:1544	phloem fibre cells	1527:1544	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	56	theme	xylan	1199:1203	arg1	components					1214:1223	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	56	theme	xylan	1199:1203	arg1	IRX10					1234:1238	IRX10	1234:1238	IRX10	1234:1238	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	56	theme	xylan	1199:1203	arg1	IRX9					1225:1228	IRX9	1225:1228	IRX9	1225:1228	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	56	theme	xylan	1199:1203	arg1	GUX1					1273:1276	xylan glucuronyl transferase GUX1	1244:1276	xylan glucuronyl transferase GUX1	1244:1276	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	57	theme	ray	1483:1485	arg1	cells					1498:1502	ray parenchyma cells	1483:1502	ray parenchyma cells	1483:1502	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	4	58	theme	increased	897:905	arg1	abundance					907:915	increased abundance	897:915	increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin	897:988	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	3	59	theme	bud	518:520	arg1	system					538:543	a tractable bud culture-derived system	506:543	a tractable bud culture-derived system	506:543	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	6	60	theme	fibre	1473:1477	arg1	xylem					1467:1471	xylem fibre	1467:1477	xylem fibre	1467:1477	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	5	61	theme	Histochemical	991:1003	arg1	staining					1005:1012	Histochemical staining	991:1012	Histochemical staining	991:1012	Histochemical staining and immunolabeling revealed that increased deposition of lignin and xylan was associated with xylem, xylem fibre cells and phloem fibre cells.
24504696	4	62	theme	substitution	849:860	arg1	frequency					836:844	the frequency	832:844	the frequency of substitution of xylan with glucuronic acid	832:890	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	6	63	theme	glucuronyl	1250:1259	arg1	GUX1					1273:1276	xylan glucuronyl transferase GUX1	1244:1276	xylan glucuronyl transferase GUX1	1244:1276	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	63	theme	glucuronyl	1250:1259	arg1	components					1214:1223	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis	1184:1291	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	64	theme	fibre	1534:1538	arg1	cells					1540:1544	phloem fibre cells	1527:1544	phloem fibre cells	1527:1544	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	7	65	theme	spatial	1684:1690	arg1	variation					1705:1713	spatial and temporal variation	1684:1713	spatial and temporal variation in xylan-related transcripts	1684:1742	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	6	66	theme	spatial	1332:1338	arg1	pattern					1340:1346	the same spatial pattern	1323:1346	the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells	1323:1544	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	7	67	theme	novel	1638:1642	arg1	information					1644:1654	novel information	1638:1654	novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition	1638:1764	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	3	68	theme	Salix	562:566	arg1	purpurea					568:575	Salix purpurea	562:575	Salix purpurea	562:575	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	0	69	from	composition	20:30	arg1	stems					100:104	developing willow (Salix purpurea) stems	65:104	developing willow (Salix purpurea) stems	65:104	Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems.
24504696	3	70	from	genes	489:493	arg1	willow					498:503	willow	498:503	willow	498:503	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	1	71	theme	cell	139:142	arg1	SCW					150:152	SCW	150:152	SCW	150:152	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	1	71	theme	cell	139:142	arg1	wall					144:147	the secondary cell wall	125:147	the secondary cell wall (SCW) in willow	125:163	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	3	72	link	culture-derived	522:536	arg1	system					538:543	a tractable bud culture-derived system	506:543	a tractable bud culture-derived system	506:543	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	0	73	theme	purpurea	90:97	arg1	stems					100:104	developing willow (Salix purpurea) stems	65:104	developing willow (Salix purpurea) stems	65:104	Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems.
24504696	1	74	from	wall	144:147	arg1	willow					158:163	willow	158:163	willow	158:163	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	2	75	theme	SCW	277:279	arg1	development					281:291	SCW development	277:291	SCW development	277:291	SCW development has been little studied in willow and it is not known how willow compares with model species, particularly the closely related genus Populus.
24504696	4	76	theme	xylan	865:869	arg1	substitution					849:860	substitution	849:860	substitution of xylan with glucuronic acid	849:890	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	0	77	from	expression	51:60	arg1	stems					100:104	developing willow (Salix purpurea) stems	65:104	developing willow (Salix purpurea) stems	65:104	Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems.
24504696	4	78	from	increase	788:795	arg1	content					806:812	xylan content	800:812	xylan content	800:812	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	4	79	with	substitution	849:860	arg1	acid					887:890	glucuronic acid	876:890	glucuronic acid	876:890	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	4	80	theme	glucuronic	876:885	arg1	acid					887:890	glucuronic acid	876:890	glucuronic acid	876:890	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	4	81	theme	period	721:726	arg1	weeks					732:736	the period 0-2 weeks	717:736	the period 0-2 weeks after transfer to soil	717:759	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	0	82	theme	cell	10:13	arg1	composition					20:30	Secondary cell wall composition	0:30	Secondary cell wall composition	0:30	Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems.
24504696	5	83	theme	increased	1047:1055	arg1	deposition					1057:1066	increased deposition	1047:1066	increased deposition of lignin and xylan	1047:1086	Histochemical staining and immunolabeling revealed that increased deposition of lignin and xylan was associated with xylem, xylem fibre cells and phloem fibre cells.
24504696	7	84	from	similarity	1572:1581	arg1	species					1615:1621	Populus species	1607:1621	Populus species	1607:1621	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	6	85	theme	in	1374:1375	arg1	hybridisation					1382:1394	in situ hybridisation	1374:1394	in situ hybridisation at four developmental stages	1374:1423	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	1	86	theme	biofuel	255:261	arg1	applications					263:274	potential bioenergy and biofuel applications	231:274	potential bioenergy and biofuel applications	231:274	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24504696	4	87	from	increase	687:694	arg1	deposition					703:712	SCW deposition	699:712	SCW deposition in the period 0-2 weeks after transfer to soil	699:759	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	7	88	theme	Populus	1607:1613	arg1	species					1615:1621	Populus species	1607:1621	Populus species	1607:1621	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	4	89	theme	SCW	958:960	arg1	cellulose					962:970	SCW cellulose	958:970	SCW cellulose	958:970	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	4	90	theme	SCW	699:701	arg1	deposition					703:712	SCW deposition	699:712	SCW deposition in the period 0-2 weeks after transfer to soil	699:759	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	0	91	theme	candidate	36:44	arg1	expression					51:60	candidate gene expression	36:60	candidate gene expression	36:60	Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems.
24504696	5	92	theme	fibre	1121:1125	arg1	cells					1127:1131	xylem fibre cells	1115:1131	xylem fibre cells	1115:1131	Histochemical staining and immunolabeling revealed that increased deposition of lignin and xylan was associated with xylem, xylem fibre cells and phloem fibre cells.
24504696	3	93	theme	cell	582:585	arg1	composition					592:602	cell wall composition	582:602	cell wall composition	582:602	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	6	94	from	IRX10	1234:1238	arg1	Arabidopsis					1281:1291	Arabidopsis	1281:1291	Arabidopsis	1281:1291	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	95	dep	in	1374:1375	arg1	situ					1377:1380	situ	1377:1380	situ	1377:1380	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	96	from	expression	1440:1449	arg1	cells					1498:1502	ray parenchyma cells	1483:1502	ray parenchyma cells	1483:1502	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	96	from	expression	1440:1449	arg1	xylem					1467:1471	xylem fibre	1467:1477	xylem fibre	1467:1477	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	96	from	expression	1440:1449	arg1	proto-xylem					1454:1464	proto-xylem	1454:1464	proto-xylem	1454:1464	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	96	from	expression	1440:1449	arg1	cells					1540:1544	phloem fibre cells	1527:1544	phloem fibre cells	1527:1544	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	4	97	theme	transcripts	929:939	arg1	abundance					907:915	increased abundance	897:915	increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin	897:988	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	4	98	from	deposition	703:712	arg1	weeks					732:736	the period 0-2 weeks	717:736	the period 0-2 weeks after transfer to soil	717:759	A large increase in SCW deposition in the period 0-2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin.
24504696	7	99	from	variation	1705:1713	arg1	transcripts					1732:1742	xylan-related transcripts	1718:1742	xylan-related transcripts	1718:1742	The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.
24504696	3	100	theme	RNA-Seq	608:614	arg1	transcriptome					616:628	RNA-Seq transcriptome	608:628	RNA-Seq transcriptome	608:628	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	6	101	from	stages	1418:1423	arg1	hybridisation					1382:1394	in situ hybridisation	1374:1394	in situ hybridisation at four developmental stages	1374:1423	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	6	102	from	IRX9	1225:1228	arg1	Arabidopsis					1281:1291	Arabidopsis	1281:1291	Arabidopsis	1281:1291	Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells.
24504696	3	103	theme	candidate	479:487	arg1	genes					489:493	candidate genes	479:493	candidate genes in willow	479:503	To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development.
24504696	1	104	theme	biomass	209:215	arg1	feedstock					217:225	willow biomass feedstock	202:225	willow biomass feedstock	202:225	The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications.
24507345	4	0	dep	network	742:748	arg1	off					735:737	off	735:737	off	735:737	Such hybrid nanostructure prevented Ag nanoparticles from dropping off BC network and thus minimized the toxicity of nanoparticles.
24507345	1	1	theme	wound	187:191	arg1	attention					166:174	increasing attention	155:174	increasing attention	155:174	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	1	1	theme	wound	187:191	arg1	material					202:209	a novel wound dressing material	179:209	a novel wound dressing material	179:209	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	0	2	theme	wound	105:109	arg1	dressing					111:118	slow-released antimicrobial wound dressing	77:118	slow-released antimicrobial wound dressing	77:118	In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.
24507345	7	3	theme	wound	1170:1174	arg1	healing					1176:1182	wound healing	1170:1182	wound healing	1170:1182	The results demonstrated that AgNP-BC could reduce inflammation and promote wound healing.
24507345	1	4	theme	dressing	193:200	arg1	attention					166:174	increasing attention	155:174	increasing attention	155:174	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	1	4	theme	dressing	193:200	arg1	material					202:209	a novel wound dressing material	179:209	a novel wound dressing material	179:209	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	1	5	theme	critical	266:273	arg1	functions					288:296	critical skin-barrier functions	266:296	critical skin-barrier functions in wound healing	266:313	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	5	6	from	reductions	915:924	arg1	coli					941:944	Escherichia coli	929:944	Escherichia coli	929:944	Regardless the slow Ag(+) release, AgNP-BC still exhibited significant antibacterial activities with more than 99% reductions in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24507345	5	6	from	reductions	915:924	arg1	aureus					962:967	Staphylococcus aureus	947:967	Staphylococcus aureus	947:967	Regardless the slow Ag(+) release, AgNP-BC still exhibited significant antibacterial activities with more than 99% reductions in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24507345	5	6	from	reductions	915:924	arg1	aeruginosa					985:994	Pseudomonas aeruginosa	973:994	Pseudomonas aeruginosa	973:994	Regardless the slow Ag(+) release, AgNP-BC still exhibited significant antibacterial activities with more than 99% reductions in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24507345	4	7	theme	nanoparticles	785:797	arg1	toxicity					773:780	the toxicity	769:780	the toxicity of nanoparticles	769:797	Such hybrid nanostructure prevented Ag nanoparticles from dropping off BC network and thus minimized the toxicity of nanoparticles.
24507345	3	8	theme	BC	574:575	arg1	nano-fibers					577:587	BC nano-fibers	574:587	BC nano-fibers	574:587	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	1	9	theme	skin-barrier	275:286	arg1	functions					288:296	critical skin-barrier functions	266:296	critical skin-barrier functions in wound healing	266:313	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	5	10	dep	%	913:913	arg1	99					911:912	99	911:912	99	911:912	Regardless the slow Ag(+) release, AgNP-BC still exhibited significant antibacterial activities with more than 99% reductions in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24507345	1	11	theme	functions	288:296	arg1	one					259:261	one	259:261	one	259:261	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	1	11	theme	functions	288:296	arg1	functions					288:296	critical skin-barrier functions	266:296	critical skin-barrier functions in wound healing	266:313	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	3	12	theme	silver	489:494	arg1	nano-particles					496:509	Uniform spherical silver nano-particles	471:509	Uniform spherical silver nano-particles (10-30 nm)	471:520	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	3	12	theme	silver	489:494	arg1	nm					518:519	10-30 nm	512:519	10-30 nm	512:519	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	4	13	theme	hybrid	673:678	arg1	nanostructure					680:692	Such hybrid nanostructure	668:692	Such hybrid nanostructure	668:692	Such hybrid nanostructure prevented Ag nanoparticles from dropping off BC network and thus minimized the toxicity of nanoparticles.
24507345	2	14	theme	silver	402:407	arg1	nanoparticles					409:421	silver nanoparticles	402:421	silver nanoparticles	402:421	To overcome such deficiency, we developed a novel method to synthesize and impregnate silver nanoparticles on to bacterial cellulose nanofibres (AgNP-BC).
24507345	6	15	with	attachment	1023:1032	arg1	cytotoxicity					1072:1083	no cytotoxicity	1069:1083	no cytotoxicity emerged	1069:1091	Moreover, AgNP-BC allowed attachment and growth of epidermal cells with no cytotoxicity emerged.
24507345	3	16	theme	nano-fibers	577:587	arg1	surface					563:569	the surface	559:569	the surface of BC nano-fibers	559:587	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	0	17	theme	In	0:1	arg1	synthesis					8:16	In situ synthesis	0:16	In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.	0:119	In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.
24507345	5	18	theme	antibacterial	871:883	arg1	activities					885:894	significant antibacterial activities	859:894	significant antibacterial activities	859:894	Regardless the slow Ag(+) release, AgNP-BC still exhibited significant antibacterial activities with more than 99% reductions in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24507345	1	19	theme	Bacterial	121:129	arg1	cellulose					131:139	Bacterial cellulose	121:139	Bacterial cellulose	121:139	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	1	20	theme	wound	301:305	arg1	healing					307:313	wound healing	301:313	wound healing	301:313	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	2	21	theme	cellulose	439:447	arg1	AgNP-BC					461:467	AgNP-BC	461:467	AgNP-BC	461:467	To overcome such deficiency, we developed a novel method to synthesize and impregnate silver nanoparticles on to bacterial cellulose nanofibres (AgNP-BC).
24507345	2	21	theme	cellulose	439:447	arg1	nanofibres					449:458	bacterial cellulose nanofibres	429:458	bacterial cellulose nanofibres (AgNP-BC)	429:468	To overcome such deficiency, we developed a novel method to synthesize and impregnate silver nanoparticles on to bacterial cellulose nanofibres (AgNP-BC).
24507345	2	22	theme	such	328:331	arg1	deficiency					333:342	such deficiency	328:342	such deficiency	328:342	To overcome such deficiency, we developed a novel method to synthesize and impregnate silver nanoparticles on to bacterial cellulose nanofibres (AgNP-BC).
24507345	1	23	from	functions	288:296	arg1	healing					307:313	wound healing	301:313	wound healing	301:313	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	2	24	theme	novel	360:364	arg1	method					366:371	a novel method	358:371	a novel method to synthesize and impregnate silver nanoparticles on to bacterial cellulose nanofibres (AgNP-BC)	358:468	To overcome such deficiency, we developed a novel method to synthesize and impregnate silver nanoparticles on to bacterial cellulose nanofibres (AgNP-BC).
24507345	2	25	theme	bacterial	429:437	arg1	AgNP-BC					461:467	AgNP-BC	461:467	AgNP-BC	461:467	To overcome such deficiency, we developed a novel method to synthesize and impregnate silver nanoparticles on to bacterial cellulose nanofibres (AgNP-BC).
24507345	2	25	theme	bacterial	429:437	arg1	nanofibres					449:458	bacterial cellulose nanofibres	429:458	bacterial cellulose nanofibres (AgNP-BC)	429:468	To overcome such deficiency, we developed a novel method to synthesize and impregnate silver nanoparticles on to bacterial cellulose nanofibres (AgNP-BC).
24507345	0	26	theme	cellulose	52:60	arg1	composites					62:71	silver-nanoparticles/bacterial cellulose composites	21:71	silver-nanoparticles/bacterial cellulose composites	21:71	In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.
24507345	3	27	dep	Ag	630:631	arg1	nanoparticles					633:645	nanoparticles	633:645	nanoparticles coated BC nanofiber	633:665	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	3	28	theme	stable	600:605	arg1	Ag					630:631	a stable and evenly distributed Ag	598:631	a stable and evenly distributed Ag nanoparticles coated BC nanofiber	598:665	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	1	29	theme	antimicrobial	226:238	arg1	activity					240:247	no antimicrobial activity	223:247	no antimicrobial activity	223:247	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	0	30	theme	silver-nanoparticles/bacterial	21:50	arg1	composites					62:71	silver-nanoparticles/bacterial cellulose composites	21:71	silver-nanoparticles/bacterial cellulose composites	21:71	In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.
24507345	5	31	theme	slow	815:818	arg1	Ag					820:821	the slow Ag	811:821	the slow Ag	811:821	Regardless the slow Ag(+) release, AgNP-BC still exhibited significant antibacterial activities with more than 99% reductions in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24507345	3	32	theme	coated	647:652	arg1	nanofiber					657:665	coated BC nanofiber	647:665	coated BC nanofiber	647:665	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	6	33	with	growth	1038:1043	arg1	cytotoxicity					1072:1083	no cytotoxicity	1069:1083	no cytotoxicity emerged	1069:1091	Moreover, AgNP-BC allowed attachment and growth of epidermal cells with no cytotoxicity emerged.
24507345	5	34	theme	significant	859:869	arg1	activities					885:894	significant antibacterial activities	859:894	significant antibacterial activities	859:894	Regardless the slow Ag(+) release, AgNP-BC still exhibited significant antibacterial activities with more than 99% reductions in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24507345	3	35	theme	Uniform	471:477	arg1	nano-particles					496:509	Uniform spherical silver nano-particles	471:509	Uniform spherical silver nano-particles (10-30 nm)	471:520	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	3	35	theme	Uniform	471:477	arg1	nm					518:519	10-30 nm	512:519	10-30 nm	512:519	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	3	36	theme	BC	654:655	arg1	nanofiber					657:665	coated BC nanofiber	647:665	coated BC nanofiber	647:665	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	5	37	dep	reductions	915:924	arg1	%					913:913	%	913:913	%	913:913	Regardless the slow Ag(+) release, AgNP-BC still exhibited significant antibacterial activities with more than 99% reductions in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24507345	0	38	theme	composites	62:71	arg1	synthesis					8:16	In situ synthesis	0:16	In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.	0:119	In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.
24507345	1	39	theme	increasing	155:164	arg1	attention					166:174	increasing attention	155:174	increasing attention	155:174	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	1	39	theme	increasing	155:164	arg1	material					202:209	a novel wound dressing material	179:209	a novel wound dressing material	179:209	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	3	40	theme	distributed	618:628	arg1	Ag					630:631	a stable and evenly distributed Ag	598:631	a stable and evenly distributed Ag nanoparticles coated BC nanofiber	598:665	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	6	41	theme	cells	1058:1062	arg1	attachment					1023:1032	attachment	1023:1032	attachment	1023:1032	Moreover, AgNP-BC allowed attachment and growth of epidermal cells with no cytotoxicity emerged.
24507345	6	41	theme	cells	1058:1062	arg1	growth					1038:1043	growth	1038:1043	growth	1038:1043	Moreover, AgNP-BC allowed attachment and growth of epidermal cells with no cytotoxicity emerged.
24507345	4	42	theme	Ag	704:705	arg1	nanoparticles					707:719	Ag nanoparticles	704:719	Ag nanoparticles	704:719	Such hybrid nanostructure prevented Ag nanoparticles from dropping off BC network and thus minimized the toxicity of nanoparticles.
24507345	6	43	theme	epidermal	1048:1056	arg1	cells					1058:1062	epidermal cells	1048:1062	epidermal cells	1048:1062	Moreover, AgNP-BC allowed attachment and growth of epidermal cells with no cytotoxicity emerged.
24507345	0	44	theme	antimicrobial	91:103	arg1	dressing					111:118	slow-released antimicrobial wound dressing	77:118	slow-released antimicrobial wound dressing	77:118	In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.
24507345	1	45	contain	has	219:221	arg2	activity					240:247	no antimicrobial activity	223:247	no antimicrobial activity	223:247	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	1	45	contain	has	219:221	arg1	it					216:217	it	216:217	it	216:217	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	3	46	theme	spherical	479:487	arg1	nano-particles					496:509	Uniform spherical silver nano-particles	471:509	Uniform spherical silver nano-particles (10-30 nm)	471:520	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	3	46	theme	spherical	479:487	arg1	nm					518:519	10-30 nm	512:519	10-30 nm	512:519	Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber.
24507345	4	47	theme	Such	668:671	arg1	nanostructure					680:692	Such hybrid nanostructure	668:692	Such hybrid nanostructure	668:692	Such hybrid nanostructure prevented Ag nanoparticles from dropping off BC network and thus minimized the toxicity of nanoparticles.
24507345	0	48	theme	slow-released	77:89	arg1	dressing					111:118	slow-released antimicrobial wound dressing	77:118	slow-released antimicrobial wound dressing	77:118	In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.
24507345	0	49	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.
24507345	1	50	theme	novel	181:185	arg1	attention					166:174	increasing attention	155:174	increasing attention	155:174	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
24507345	1	50	theme	novel	181:185	arg1	material					202:209	a novel wound dressing material	179:209	a novel wound dressing material	179:209	Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing.
25274863	9	0	theme	sheath	1486:1491	arg1	size					1493:1496	the sheath size	1482:1496	the sheath size	1482:1496	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	10	1	theme	vascular	1718:1725	arg1	events					1727:1732	severe vascular events	1711:1732	severe vascular events	1711:1732	CONCLUSION BAV performed without heparin is associated with a reduction of severe vascular events or death without increased ischemic risk.
25274863	5	2	theme	primary	664:670	arg1	endpoint					681:688	Our primary combined endpoint	660:688	Our primary combined endpoint	660:688	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	8	3	dep	risk	1363:1366	arg1	0.34-9.61					1400:1408	0.34-9.61	1400:1408	0.34-9.61	1400:1408	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	8	3	dep	risk	1363:1366	arg1	%					1377:1377	1.81, 95%	1369:1377	1.81, 95% confidence interval	1369:1397	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	10	4	theme	ischemic	1761:1768	arg1	risk					1770:1773	increased ischemic risk	1751:1773	increased ischemic risk	1751:1773	CONCLUSION BAV performed without heparin is associated with a reduction of severe vascular events or death without increased ischemic risk.
25274863	7	5	theme	ischemic	1040:1047	arg1	events					1049:1054	Vascular, bleeding, or ischemic events	1017:1054	Vascular, bleeding, or ischemic events	1017:1054	Vascular, bleeding, or ischemic events were dramatically lower in the heparin-free group (6.5%) compared with the heparin group (18.8%; P=.01).
25274863	6	6	theme	composite	875:883	arg1	endpoint					885:892	The primary composite endpoint	863:892	The primary composite endpoint	863:892	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	6	6	theme	composite	875:883	arg1	lower					960:964	lower	960:964	lower	960:964	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	4	7	theme	IU/kg	581:585	arg1	bolus					587:591	unfractionated heparin (UH) 50 IU/kg bolus IV	550:594	unfractionated heparin (UH) 50 IU/kg bolus IV	550:594	Eighty-five patients had unfractionated heparin (UH) 50 IU/kg bolus IV during the procedure, whereas 77 patients did not have heparin.
25274863	1	8	theme	aortic	206:211	arg1	BAV					228:230	BAV	228:230	BAV	228:230	OBJECTIVES To determine whether vascular and bleeding complications may be reduced with balloon aortic valvuloplasty (BAV) performed without heparin.
25274863	1	8	theme	aortic	206:211	arg1	valvuloplasty					213:225	balloon aortic valvuloplasty	198:225	balloon aortic valvuloplasty (BAV) performed without heparin	198:257	OBJECTIVES To determine whether vascular and bleeding complications may be reduced with balloon aortic valvuloplasty (BAV) performed without heparin.
25274863	5	9	dep	events	774:779	arg1	ischemia					793:800	acute limb ischemia	782:800	acute limb ischemia	782:800	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	5	9	dep	events	774:779	arg1	embolism					814:821	systemic embolism	805:821	systemic embolism	805:821	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	5	9	dep	events	774:779	arg1	events					774:779	severe ischemic events	758:779	severe ischemic events (acute limb ischemia or systemic embolism)	758:822	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	2	10	theme	main	325:328	arg1	Vascular					271:278	Vascular	271:278	Vascular	271:278	BACKGROUND Vascular and bleeding complications are currently the main adverse events occurring after BAV.
25274863	2	10	theme	main	325:328	arg1	complications					293:305	BACKGROUND Vascular and bleeding complications	260:305	complications	293:305	BACKGROUND Vascular and bleeding complications are currently the main adverse events occurring after BAV.
25274863	2	10	theme	main	325:328	arg1	events					338:343	the main adverse events	321:343	the main adverse events occurring after BAV	321:363	BACKGROUND Vascular and bleeding complications are currently the main adverse events occurring after BAV.
25274863	9	11	dep	ratio	1585:1589	arg1	1.75-14.74					1623:1632	1.75-14.74	1623:1632	1.75-14.74	1623:1632	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	9	11	dep	ratio	1585:1589	arg1	interval					1613:1620	95% confidence interval	1598:1620	95% confidence interval	1598:1620	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	11	12	theme	marked	1781:1786	arg1	difference					1788:1797	This marked difference	1776:1797	This marked difference	1776:1797	This marked difference is difficult to explain by confounding factors, but should be confirmed in a randomized controlled trial.
25274863	9	13	theme	primary	1553:1559	arg1	endpoint					1561:1568	the primary endpoint	1549:1568	the primary endpoint	1549:1568	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	11	14	theme	controlled	1887:1896	arg1	trial					1898:1902	a randomized controlled trial	1874:1902	a randomized controlled trial	1874:1902	This marked difference is difficult to explain by confounding factors, but should be confirmed in a randomized controlled trial.
25274863	1	15	theme	vascular	142:149	arg1	complications					164:176	vascular and bleeding complications	142:176	complications	164:176	OBJECTIVES To determine whether vascular and bleeding complications may be reduced with balloon aortic valvuloplasty (BAV) performed without heparin.
25274863	9	16	theme	odds	1580:1583	arg1	2.94					1592:1595	2.94	1592:1595	2.94	1592:1595	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	9	16	theme	odds	1580:1583	arg1	ratio					1585:1589	adjusted odds ratio	1571:1589	adjusted odds ratio	1571:1589	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	8	17	theme	ischemic	1337:1344	arg1	events					1346:1351	ischemic events	1337:1351	ischemic events	1337:1351	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	8	18	theme	vascular	1209:1216	arg1	complications					1231:1243	vascular and bleeding complications	1209:1243	complications	1231:1243	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	8	18	theme	vascular	1209:1216	arg1	risk					1255:1258	relative risk	1246:1258	relative risk	1246:1258	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	0	19	theme	aortic	62:67	arg1	valvuloplasty					69:81	balloon aortic valvuloplasty	54:81	balloon aortic valvuloplasty	54:81	Decrease of vascular and bleeding complications after balloon aortic valvuloplasty performed without heparin.
25274863	4	20	theme	Eighty-five	525:535	arg1	patients					537:544	Eighty-five patients	525:544	Eighty-five patients	525:544	Eighty-five patients had unfractionated heparin (UH) 50 IU/kg bolus IV during the procedure, whereas 77 patients did not have heparin.
25274863	8	21	theme	bleeding	1222:1229	arg1	complications					1231:1243	vascular and bleeding complications	1209:1243	complications	1231:1243	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	8	21	theme	bleeding	1222:1229	arg1	risk					1255:1258	relative risk	1246:1258	relative risk	1246:1258	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	9	22	dep	predictor	1536:1544	arg1	2.94					1592:1595	2.94	1592:1595	2.94	1592:1595	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	9	22	dep	predictor	1536:1544	arg1	ratio					1585:1589	adjusted odds ratio	1571:1589	adjusted odds ratio	1571:1589	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	6	23	dep	lower	960:964	arg1	%					997:997	10.3%	993:997	10.3%	993:997	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	6	23	dep	lower	960:964	arg1	%					1006:1006	20.0%	1002:1006	20.0%	1002:1006	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	6	24	theme	heparin-free	973:984	arg1	group					986:990	the heparin-free group	969:990	the heparin-free group	969:990	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	7	25	theme	heparin	1131:1137	arg1	group					1139:1143	the heparin group	1127:1143	the heparin group (18.8%; P=.01)	1127:1158	Vascular, bleeding, or ischemic events were dramatically lower in the heparin-free group (6.5%) compared with the heparin group (18.8%; P=.01).
25274863	9	26	theme	%	1600:1600	arg1	interval					1613:1620	95% confidence interval	1598:1620	95% confidence interval	1598:1620	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	3	27	theme	femoral	471:477	arg1	approach					479:486	femoral approach	471:486	femoral approach in our center between 2008 and 2012	471:522	METHODS This registry included 162 consecutive patients with severe aortic stenosis who underwent BAV by femoral approach in our center between 2008 and 2012.
25274863	5	28	theme	acute	782:786	arg1	ischemia					793:800	acute limb ischemia	782:800	acute limb ischemia	782:800	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	5	28	theme	acute	782:786	arg1	events					774:779	severe ischemic events	758:779	severe ischemic events (acute limb ischemia or systemic embolism)	758:822	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	6	29	dep	%	997:997	arg1	P=.03					1009:1013	P=.03	1009:1013	P=.03	1009:1013	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	3	30	theme	consecutive	401:411	arg1	patients					413:420	162 consecutive patients	397:420	162 consecutive patients with severe aortic stenosis who underwent BAV by femoral approach in our center between 2008 and 2012	397:522	METHODS This registry included 162 consecutive patients with severe aortic stenosis who underwent BAV by femoral approach in our center between 2008 and 2012.
25274863	7	31	dep	group	1139:1143	arg1	%					1150:1150	18.8%	1146:1150	18.8%; P=.01	1146:1157	Vascular, bleeding, or ischemic events were dramatically lower in the heparin-free group (6.5%) compared with the heparin group (18.8%; P=.01).
25274863	0	32	theme	vascular	12:19	arg1	complications					34:46	vascular and bleeding complications	12:46	vascular and bleeding complications after balloon aortic valvuloplasty	12:81	Decrease of vascular and bleeding complications after balloon aortic valvuloplasty performed without heparin.
25274863	6	33	theme	primary	867:873	arg1	endpoint					885:892	The primary composite endpoint	863:892	The primary composite endpoint	863:892	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	6	33	theme	primary	867:873	arg1	lower					960:964	lower	960:964	lower	960:964	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	0	34	theme	bleeding	25:32	arg1	complications					34:46	vascular and bleeding complications	12:46	vascular and bleeding complications after balloon aortic valvuloplasty	12:81	Decrease of vascular and bleeding complications after balloon aortic valvuloplasty performed without heparin.
25274863	5	35	theme	limb	788:791	arg1	ischemia					793:800	acute limb ischemia	782:800	acute limb ischemia	782:800	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	5	35	theme	limb	788:791	arg1	events					774:779	severe ischemic events	758:779	severe ischemic events (acute limb ischemia or systemic embolism)	758:822	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	9	36	theme	patient	1433:1439	arg1	size					1493:1496	the sheath size	1482:1496	the sheath size	1482:1496	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	9	36	theme	patient	1433:1439	arg1	characteristics					1455:1469	patient and procedure characteristics	1433:1469	patient and procedure characteristics	1433:1469	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	3	37	theme	aortic	434:439	arg1	stenosis					441:448	severe aortic stenosis	427:448	severe aortic stenosis	427:448	METHODS This registry included 162 consecutive patients with severe aortic stenosis who underwent BAV by femoral approach in our center between 2008 and 2012.
25274863	4	38	theme	heparin	565:571	arg1	bolus					587:591	unfractionated heparin (UH) 50 IU/kg bolus IV	550:594	unfractionated heparin (UH) 50 IU/kg bolus IV	550:594	Eighty-five patients had unfractionated heparin (UH) 50 IU/kg bolus IV during the procedure, whereas 77 patients did not have heparin.
25274863	4	39	contain	have	646:649	arg1	patients					629:636	77 patients	626:636	77 patients	626:636	Eighty-five patients had unfractionated heparin (UH) 50 IU/kg bolus IV during the procedure, whereas 77 patients did not have heparin.
25274863	4	39	contain	have	646:649	arg2	heparin					651:657	heparin	651:657	heparin	651:657	Eighty-five patients had unfractionated heparin (UH) 50 IU/kg bolus IV during the procedure, whereas 77 patients did not have heparin.
25274863	3	40	dep	METHODS	366:372	arg1	included					388:395	included	388:395	included 162 consecutive patients with severe aortic stenosis who underwent BAV by femoral approach in our center between 2008 and 2012	388:522	METHODS This registry included 162 consecutive patients with severe aortic stenosis who underwent BAV by femoral approach in our center between 2008 and 2012.
25274863	9	41	theme	procedure	1445:1453	arg1	size					1493:1496	the sheath size	1482:1496	the sheath size	1482:1496	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	9	41	theme	procedure	1445:1453	arg1	characteristics					1455:1469	patient and procedure characteristics	1433:1469	patient and procedure characteristics	1433:1469	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	5	42	theme	ischemic	765:772	arg1	ischemia					793:800	acute limb ischemia	782:800	acute limb ischemia	782:800	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	5	42	theme	ischemic	765:772	arg1	embolism					814:821	systemic embolism	805:821	systemic embolism	805:821	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	5	42	theme	ischemic	765:772	arg1	events					774:779	severe ischemic events	758:779	severe ischemic events (acute limb ischemia or systemic embolism)	758:822	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	4	43	contain	had	546:548	arg1	patients					537:544	Eighty-five patients	525:544	Eighty-five patients	525:544	Eighty-five patients had unfractionated heparin (UH) 50 IU/kg bolus IV during the procedure, whereas 77 patients did not have heparin.
25274863	4	43	contain	had	546:548	arg2	bolus					587:591	unfractionated heparin (UH) 50 IU/kg bolus IV	550:594	unfractionated heparin (UH) 50 IU/kg bolus IV	550:594	Eighty-five patients had unfractionated heparin (UH) 50 IU/kg bolus IV during the procedure, whereas 77 patients did not have heparin.
25274863	6	44	theme	overall	922:928	arg1	%					935:935	15.4%	931:935	15.4%	931:935	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	6	44	theme	overall	922:928	arg1	patients					913:920	25 patients overall	910:928	25 patients overall (15.4%)	910:936	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	10	45	theme	events	1727:1732	arg1	reduction					1698:1706	a reduction	1696:1706	a reduction of severe vascular events or death without increased ischemic risk	1696:1773	CONCLUSION BAV performed without heparin is associated with a reduction of severe vascular events or death without increased ischemic risk.
25274863	3	46	from	approach	479:486	arg1	center					495:500	our center	491:500	our center between 2008 and 2012	491:522	METHODS This registry included 162 consecutive patients with severe aortic stenosis who underwent BAV by femoral approach in our center between 2008 and 2012.
25274863	10	47	theme	severe	1711:1716	arg1	events					1727:1732	severe vascular events	1711:1732	severe vascular events	1711:1732	CONCLUSION BAV performed without heparin is associated with a reduction of severe vascular events or death without increased ischemic risk.
25274863	5	48	theme	combined	672:679	arg1	endpoint					681:688	Our primary combined endpoint	660:688	Our primary combined endpoint	660:688	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	11	49	theme	confounding	1826:1836	arg1	factors					1838:1844	confounding factors	1826:1844	confounding factors	1826:1844	This marked difference is difficult to explain by confounding factors, but should be confirmed in a randomized controlled trial.
25274863	9	50	theme	heparin	1499:1505	arg1	predictor					1536:1544	the only significant predictor	1515:1544	the only significant predictor of the primary endpoint	1515:1568	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	9	50	theme	heparin	1499:1505	arg1	use					1507:1509	heparin use	1499:1509	heparin use	1499:1509	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	10	51	theme	increased	1751:1759	arg1	risk					1770:1773	increased ischemic risk	1751:1773	increased ischemic risk	1751:1773	CONCLUSION BAV performed without heparin is associated with a reduction of severe vascular events or death without increased ischemic risk.
25274863	10	52	theme	death	1737:1741	arg1	reduction					1698:1706	a reduction	1696:1706	a reduction of severe vascular events or death without increased ischemic risk	1696:1773	CONCLUSION BAV performed without heparin is associated with a reduction of severe vascular events or death without increased ischemic risk.
25274863	7	53	theme	bleeding	1027:1034	arg1	events					1049:1054	Vascular, bleeding, or ischemic events	1017:1054	Vascular, bleeding, or ischemic events	1017:1054	Vascular, bleeding, or ischemic events were dramatically lower in the heparin-free group (6.5%) compared with the heparin group (18.8%; P=.01).
25274863	1	54	theme	balloon	198:204	arg1	BAV					228:230	BAV	228:230	BAV	228:230	OBJECTIVES To determine whether vascular and bleeding complications may be reduced with balloon aortic valvuloplasty (BAV) performed without heparin.
25274863	1	54	theme	balloon	198:204	arg1	valvuloplasty					213:225	balloon aortic valvuloplasty	198:225	balloon aortic valvuloplasty (BAV) performed without heparin	198:257	OBJECTIVES To determine whether vascular and bleeding complications may be reduced with balloon aortic valvuloplasty (BAV) performed without heparin.
25274863	3	55	with	patients	413:420	arg1	stenosis					441:448	severe aortic stenosis	427:448	severe aortic stenosis	427:448	METHODS This registry included 162 consecutive patients with severe aortic stenosis who underwent BAV by femoral approach in our center between 2008 and 2012.
25274863	7	56	theme	Vascular	1017:1024	arg1	events					1049:1054	Vascular, bleeding, or ischemic events	1017:1054	Vascular, bleeding, or ischemic events	1017:1054	Vascular, bleeding, or ischemic events were dramatically lower in the heparin-free group (6.5%) compared with the heparin group (18.8%; P=.01).
25274863	2	57	theme	adverse	330:336	arg1	Vascular					271:278	Vascular	271:278	Vascular	271:278	BACKGROUND Vascular and bleeding complications are currently the main adverse events occurring after BAV.
25274863	2	57	theme	adverse	330:336	arg1	complications					293:305	BACKGROUND Vascular and bleeding complications	260:305	complications	293:305	BACKGROUND Vascular and bleeding complications are currently the main adverse events occurring after BAV.
25274863	2	57	theme	adverse	330:336	arg1	events					338:343	the main adverse events	321:343	the main adverse events occurring after BAV	321:363	BACKGROUND Vascular and bleeding complications are currently the main adverse events occurring after BAV.
25274863	8	58	dep	risk	1255:1258	arg1	%					1269:1269	2.92; 95%	1261:1269	2.92; 95% confidence interval	1261:1289	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	8	58	dep	risk	1255:1258	arg1	1.35-7.94					1292:1300	1.35-7.94	1292:1300	1.35-7.94	1292:1300	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	11	59	theme	randomized	1876:1885	arg1	trial					1898:1902	a randomized controlled trial	1874:1902	a randomized controlled trial	1874:1902	This marked difference is difficult to explain by confounding factors, but should be confirmed in a randomized controlled trial.
25274863	8	60	theme	UH	1161:1162	arg1	use					1164:1166	UH use	1161:1166	UH use	1161:1166	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	5	61	theme	1-month	836:842	arg1	follow-up					844:852	1-month follow-up	836:852	1-month follow-up	836:852	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	8	62	theme	decreased	1319:1327	arg1	risk					1329:1332	a decreased risk	1317:1332	a decreased risk of ischemic events	1317:1351	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	5	63	from	follow-up	844:852	arg1	death					827:831	death	827:831	death at 1-month follow-up	827:852	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	9	64	theme	endpoint	1561:1568	arg1	predictor					1536:1544	the only significant predictor	1515:1544	the only significant predictor of the primary endpoint	1515:1568	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	9	64	theme	endpoint	1561:1568	arg1	use					1507:1509	heparin use	1499:1509	heparin use	1499:1509	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	2	65	theme	bleeding	284:291	arg1	Vascular					271:278	Vascular	271:278	Vascular	271:278	BACKGROUND Vascular and bleeding complications are currently the main adverse events occurring after BAV.
25274863	2	65	theme	bleeding	284:291	arg1	complications					293:305	BACKGROUND Vascular and bleeding complications	260:305	complications	293:305	BACKGROUND Vascular and bleeding complications are currently the main adverse events occurring after BAV.
25274863	2	65	theme	bleeding	284:291	arg1	events					338:343	the main adverse events	321:343	the main adverse events occurring after BAV	321:363	BACKGROUND Vascular and bleeding complications are currently the main adverse events occurring after BAV.
25274863	0	66	theme	complications	34:46	arg1	Decrease					0:7	Decrease	0:7	Decrease of vascular and bleeding complications after balloon aortic valvuloplasty	0:81	Decrease of vascular and bleeding complications after balloon aortic valvuloplasty performed without heparin.
25274863	9	67	theme	adjusted	1571:1578	arg1	2.94					1592:1595	2.94	1592:1595	2.94	1592:1595	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	9	67	theme	adjusted	1571:1578	arg1	ratio					1585:1589	adjusted odds ratio	1571:1589	adjusted odds ratio	1571:1589	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	8	68	theme	events	1346:1351	arg1	risk					1329:1332	a decreased risk	1317:1332	a decreased risk of ischemic events	1317:1351	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	8	69	theme	increased	1191:1199	arg1	risk					1201:1204	an increased risk	1188:1204	an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94)	1188:1301	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	5	70	theme	severe	758:763	arg1	ischemia					793:800	acute limb ischemia	782:800	acute limb ischemia	782:800	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	5	70	theme	severe	758:763	arg1	embolism					814:821	systemic embolism	805:821	systemic embolism	805:821	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	5	70	theme	severe	758:763	arg1	events					774:779	severe ischemic events	758:779	severe ischemic events (acute limb ischemia or systemic embolism)	758:822	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	10	71	theme	CONCLUSION	1636:1645	arg1	BAV					1647:1649	CONCLUSION BAV	1636:1649	CONCLUSION BAV performed without heparin	1636:1675	CONCLUSION BAV performed without heparin is associated with a reduction of severe vascular events or death without increased ischemic risk.
25274863	1	72	theme	bleeding	155:162	arg1	complications					164:176	vascular and bleeding complications	142:176	complications	164:176	OBJECTIVES To determine whether vascular and bleeding complications may be reduced with balloon aortic valvuloplasty (BAV) performed without heparin.
25274863	8	73	theme	relative	1354:1361	arg1	risk					1363:1366	relative risk	1354:1366	relative risk	1354:1366	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	5	74	theme	bleeding	718:725	arg1	complications					727:739	severe vascular or bleeding complications	699:739	complications	727:739	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	4	75	theme	unfractionated	550:563	arg1	UH					574:575	UH	574:575	UH	574:575	Eighty-five patients had unfractionated heparin (UH) 50 IU/kg bolus IV during the procedure, whereas 77 patients did not have heparin.
25274863	4	75	theme	unfractionated	550:563	arg1	heparin					565:571	unfractionated heparin	550:571	unfractionated heparin (UH) 50 IU/kg bolus IV	550:594	Eighty-five patients had unfractionated heparin (UH) 50 IU/kg bolus IV during the procedure, whereas 77 patients did not have heparin.
25274863	8	76	dep	associated	1172:1181	arg1	risk					1363:1366	relative risk	1354:1366	relative risk	1354:1366	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	9	77	theme	95	1598:1599	arg1	%					1600:1600	%	1600:1600	%	1600:1600	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	8	78	theme	complications	1231:1243	arg1	risk					1201:1204	an increased risk	1188:1204	an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94)	1188:1301	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	9	79	theme	confidence	1602:1611	arg1	interval					1613:1620	95% confidence interval	1598:1620	95% confidence interval	1598:1620	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	8	80	theme	relative	1246:1253	arg1	complications					1231:1243	vascular and bleeding complications	1209:1243	complications	1231:1243	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	8	80	theme	relative	1246:1253	arg1	risk					1255:1258	relative risk	1246:1258	relative risk	1246:1258	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	5	81	theme	systemic	805:812	arg1	embolism					814:821	systemic embolism	805:821	systemic embolism	805:821	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	5	81	theme	systemic	805:812	arg1	events					774:779	severe ischemic events	758:779	severe ischemic events (acute limb ischemia or systemic embolism)	758:822	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	3	82	theme	severe	427:432	arg1	stenosis					441:448	severe aortic stenosis	427:448	severe aortic stenosis	427:448	METHODS This registry included 162 consecutive patients with severe aortic stenosis who underwent BAV by femoral approach in our center between 2008 and 2012.
25274863	8	83	theme	interval	1390:1397	arg1	%					1377:1377	1.81, 95%	1369:1377	1.81, 95% confidence interval	1369:1397	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	7	84	theme	heparin-free	1087:1098	arg1	group					1100:1104	the heparin-free group	1083:1104	the heparin-free group (6.5%)	1083:1111	Vascular, bleeding, or ischemic events were dramatically lower in the heparin-free group (6.5%) compared with the heparin group (18.8%; P=.01).
25274863	7	84	theme	heparin-free	1087:1098	arg1	%					1110:1110	6.5%	1107:1110	6.5%	1107:1110	Vascular, bleeding, or ischemic events were dramatically lower in the heparin-free group (6.5%) compared with the heparin group (18.8%; P=.01).
25274863	6	85	dep	RESULTS	855:861	arg1	achieved					898:905	achieved	898:905	was achieved in 25 patients overall (15.4%)	894:936	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	6	85	dep	RESULTS	855:861	arg1	endpoint					885:892	The primary composite endpoint	863:892	The primary composite endpoint	863:892	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	6	85	dep	RESULTS	855:861	arg1	lower					960:964	lower	960:964	lower	960:964	RESULTS The primary composite endpoint was achieved in 25 patients overall (15.4%) and was significantly lower in the heparin-free group (10.3% vs 20.0%; P=.03).
25274863	7	86	dep	%	1150:1150	arg1	P=.01					1153:1157	P=.01	1153:1157	18.8%; P=.01	1146:1157	Vascular, bleeding, or ischemic events were dramatically lower in the heparin-free group (6.5%) compared with the heparin group (18.8%; P=.01).
25274863	0	87	theme	balloon	54:60	arg1	valvuloplasty					69:81	balloon aortic valvuloplasty	54:81	balloon aortic valvuloplasty	54:81	Decrease of vascular and bleeding complications after balloon aortic valvuloplasty performed without heparin.
25274863	5	88	theme	BARC	742:745	arg1	vascular					706:713	vascular	706:713	vascular	706:713	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	5	88	theme	BARC	742:745	arg1	score					747:751	BARC score ≥3	742:754	BARC score ≥3	742:754	Our primary combined endpoint included severe vascular or bleeding complications (BARC score ≥3), severe ischemic events (acute limb ischemia or systemic embolism) or death at 1-month follow-up.
25274863	8	89	theme	interval	1282:1289	arg1	%					1269:1269	2.92; 95%	1261:1269	2.92; 95% confidence interval	1261:1289	UH use was associated with an increased risk of vascular and bleeding complications (relative risk, 2.92; 95% confidence interval, 1.35-7.94), but not with a decreased risk of ischemic events (relative risk, 1.81, 95% confidence interval, 0.34-9.61).
25274863	9	90	theme	significant	1524:1534	arg1	predictor					1536:1544	the only significant predictor	1515:1544	the only significant predictor of the primary endpoint	1515:1568	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
25274863	9	90	theme	significant	1524:1534	arg1	use					1507:1509	heparin use	1499:1509	heparin use	1499:1509	After adjustment for patient and procedure characteristics, including the sheath size, heparin use was the only significant predictor of the primary endpoint (adjusted odds ratio, 2.94; 95% confidence interval, 1.75-14.74).
26226692	0	0	theme	connective	80:89	arg1	tissue					91:96	connective tissue	80:96	connective tissue	80:96	[The effect of the biopolymer chondroitin sulfate on reparative regeneration of connective tissue].
26226692	1	1	theme	reparative	227:236	arg1	regeneration					238:249	reparative regeneration	227:249	reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis	227:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	2	2	theme	sulfate	396:402	arg1	maintenance					369:379	intra-articular maintenance	353:379	intra-articular maintenance of chondroitin sulfate (the preparation "mukosat")	353:430	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	2	theme	sulfate	396:402	arg1	stimulus					442:449	a stimulus	440:449	a stimulus for reparative regeneration of connective tissue	440:498	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	0	3	from	effect	5:10	arg1	regeneration					64:75	reparative regeneration	53:75	reparative regeneration of connective tissue	53:96	[The effect of the biopolymer chondroitin sulfate on reparative regeneration of connective tissue].
26226692	2	4	theme	joints	580:585	arg1	elements					568:575	connective tissue elements	550:575	connective tissue elements of joints	550:585	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	5	theme	preparation	409:419	arg1	sulfate					396:402	chondroitin sulfate	384:402	chondroitin sulfate (the preparation "mukosat")	384:430	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	5	theme	preparation	409:419	arg1	"					429:429	the preparation "mukosat"	405:429	the preparation "mukosat"	405:429	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	1	6	theme	intra-articular	140:154	arg1	method					156:161	an intra-articular method	137:161	an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis	137:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	2	7	theme	elements	568:575	arg1	status					540:545	the status	536:545	the status of connective tissue elements of joints	536:585	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	1	8	theme	regeneration	238:249	arg1	stimulation					212:222	stimulation	212:222	stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis	212:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	2	9	dep	ascertained	336:346	arg1	decrease					588:595	decrease	588:595	decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component	588:720	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	3	10	theme	knee	835:838	arg1	joints					840:845	animal knee joints	828:845	animal knee joints	828:845	It probably depends on stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints.
26226692	0	11	theme	tissue	91:96	arg1	regeneration					64:75	reparative regeneration	53:75	reparative regeneration of connective tissue	53:96	[The effect of the biopolymer chondroitin sulfate on reparative regeneration of connective tissue].
26226692	1	12	from	rabbits	290:296	arg1	tissue					265:270	connective tissue	254:270	connective tissue of knee joints in rabbits with an experimental arthritis	254:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	2	13	theme	blood	629:633	arg1	serum					635:639	blood serum	629:639	blood serum	629:639	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	1	14	theme	connective	254:263	arg1	tissue					265:270	connective tissue	254:270	connective tissue of knee joints in rabbits with an experimental arthritis	254:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	1	15	with	rabbits	290:296	arg1	arthritis					319:327	an experimental arthritis	303:327	an experimental arthritis	303:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	1	16	theme	method	156:161	arg1	objective					113:121	The research objective	100:121	The research objective	100:121	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	1	16	theme	method	156:161	arg1	study					128:132	a study	126:132	a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis	126:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	2	17	theme	tissue	493:498	arg1	regeneration					466:477	reparative regeneration	455:477	reparative regeneration of connective tissue	455:498	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	1	18	from	joints	280:285	arg1	rabbits					290:296	rabbits	290:296	rabbits with an experimental arthritis	290:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	2	19	theme	composition	666:676	arg1	serum					635:639	blood serum	629:639	blood serum	629:639	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	19	theme	composition	666:676	arg1	normalization					645:657	normalization	645:657	normalization of the composition of glycosaminoglycan carbohydrate component	645:720	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	20	theme	chondroitin	384:394	arg1	sulfate					396:402	chondroitin sulfate	384:402	chondroitin sulfate (the preparation "mukosat")	384:430	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	20	theme	chondroitin	384:394	arg1	"					429:429	the preparation "mukosat"	405:429	the preparation "mukosat"	405:429	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	21	theme	reparative	455:464	arg1	regeneration					466:477	reparative regeneration	455:477	reparative regeneration of connective tissue	455:498	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	1	22	from	tissue	265:270	arg1	rabbits					290:296	rabbits	290:296	rabbits with an experimental arthritis	290:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	3	23	from	tissues	817:823	arg1	stimulation					746:756	stimulation	746:756	stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints	746:845	It probably depends on stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints.
26226692	1	24	theme	introduction	166:177	arg1	method					156:161	an intra-articular method	137:161	an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis	137:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	0	25	theme	[The	0:3	arg1	effect					5:10	[The effect	0:10	[The effect of the biopolymer chondroitin sulfate on reparative regeneration of connective tissue	0:96	[The effect of the biopolymer chondroitin sulfate on reparative regeneration of connective tissue].
26226692	1	26	theme	tissue	265:270	arg1	regeneration					238:249	reparative regeneration	227:249	reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis	227:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	3	27	from	biosynthesis	761:772	arg1	tissues					817:823	tissues	817:823	tissues of animal knee joints	817:845	It probably depends on stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints.
26226692	3	28	theme	biosynthesis	761:772	arg1	stimulation					746:756	stimulation	746:756	stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints	746:845	It probably depends on stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints.
26226692	3	29	theme	joints	840:845	arg1	tissues					817:823	tissues	817:823	tissues of animal knee joints	817:845	It probably depends on stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints.
26226692	2	30	theme	mukosat	422:428	arg1	sulfate					396:402	chondroitin sulfate	384:402	chondroitin sulfate (the preparation "mukosat")	384:430	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	30	theme	mukosat	422:428	arg1	"					429:429	the preparation "mukosat"	405:429	the preparation "mukosat"	405:429	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	1	31	theme	knee	275:278	arg1	joints					280:285	knee joints	275:285	knee joints in rabbits with an experimental arthritis	275:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	1	32	theme	preparation	186:196	arg1	"					206:206	the preparation "mukosat"	182:206	the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis	182:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	0	33	theme	chondroitin	30:40	arg1	sulfate					42:48	the biopolymer chondroitin sulfate	15:48	the biopolymer chondroitin sulfate	15:48	[The effect of the biopolymer chondroitin sulfate on reparative regeneration of connective tissue].
26226692	1	34	theme	joints	280:285	arg1	tissue					265:270	connective tissue	254:270	connective tissue of knee joints in rabbits with an experimental arthritis	254:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	0	35	theme	biopolymer	19:28	arg1	sulfate					42:48	the biopolymer chondroitin sulfate	15:48	the biopolymer chondroitin sulfate	15:48	[The effect of the biopolymer chondroitin sulfate on reparative regeneration of connective tissue].
26226692	3	36	theme	normal	788:793	arg1	glycosaminoglycans					795:812	autologous normal glycosaminoglycans	777:812	autologous normal glycosaminoglycans	777:812	It probably depends on stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints.
26226692	2	37	theme	positive	516:523	arg1	changes					525:531	positive changes	516:531	positive changes in the status of connective tissue elements of joints	516:585	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	38	theme	component	712:720	arg1	composition					666:676	the composition	662:676	the composition of glycosaminoglycan carbohydrate component	662:720	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	1	39	theme	mukosat	199:205	arg1	"					206:206	the preparation "mukosat"	182:206	the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis	182:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	2	40	from	decrease	588:595	arg1	content					618:624	glycosaminoglycan content	600:624	glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component	600:720	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	41	theme	tissue	561:566	arg1	elements					568:575	connective tissue elements	550:575	connective tissue elements of joints	550:585	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	42	theme	carbohydrate	699:710	arg1	component					712:720	glycosaminoglycan carbohydrate component	681:720	glycosaminoglycan carbohydrate component	681:720	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	1	43	theme	research	104:111	arg1	objective					113:121	The research objective	100:121	The research objective	100:121	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	1	43	theme	research	104:111	arg1	study					128:132	a study	126:132	a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis	126:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	0	44	theme	sulfate	42:48	arg1	effect					5:10	[The effect	0:10	[The effect of the biopolymer chondroitin sulfate on reparative regeneration of connective tissue	0:96	[The effect of the biopolymer chondroitin sulfate on reparative regeneration of connective tissue].
26226692	2	45	from	content	618:624	arg1	serum					635:639	blood serum	629:639	blood serum	629:639	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	45	from	content	618:624	arg1	normalization					645:657	normalization	645:657	normalization of the composition of glycosaminoglycan carbohydrate component	645:720	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	46	theme	connective	550:559	arg1	elements					568:575	connective tissue elements	550:575	connective tissue elements of joints	550:585	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	47	theme	glycosaminoglycan	600:616	arg1	content					618:624	glycosaminoglycan content	600:624	glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component	600:720	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	48	theme	glycosaminoglycan	681:697	arg1	component					712:720	glycosaminoglycan carbohydrate component	681:720	glycosaminoglycan carbohydrate component	681:720	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	3	49	theme	autologous	777:786	arg1	glycosaminoglycans					795:812	autologous normal glycosaminoglycans	777:812	autologous normal glycosaminoglycans	777:812	It probably depends on stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints.
26226692	1	50	theme	"	206:206	arg1	introduction					166:177	introduction	166:177	introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis	166:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	3	51	theme	animal	828:833	arg1	joints					840:845	animal knee joints	828:845	animal knee joints	828:845	It probably depends on stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints.
26226692	2	52	theme	connective	482:491	arg1	tissue					493:498	connective tissue	482:498	connective tissue	482:498	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	3	53	from	stimulation	746:756	arg1	tissues					817:823	tissues	817:823	tissues of animal knee joints	817:845	It probably depends on stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints.
26226692	2	54	theme	intra-articular	353:367	arg1	maintenance					369:379	intra-articular maintenance	353:379	intra-articular maintenance of chondroitin sulfate (the preparation "mukosat")	353:430	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	2	54	theme	intra-articular	353:367	arg1	stimulus					442:449	a stimulus	440:449	a stimulus for reparative regeneration of connective tissue	440:498	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	0	55	theme	reparative	53:62	arg1	regeneration					64:75	reparative regeneration	53:75	reparative regeneration of connective tissue	53:96	[The effect of the biopolymer chondroitin sulfate on reparative regeneration of connective tissue].
26226692	1	56	theme	experimental	306:317	arg1	arthritis					319:327	an experimental arthritis	303:327	an experimental arthritis	303:327	The research objective is a study of an intra-articular method of introduction of the preparation "mukosat" for stimulation of reparative regeneration of connective tissue of knee joints in rabbits with an experimental arthritis.
26226692	2	57	from	changes	525:531	arg1	status					540:545	the status	536:545	the status of connective tissue elements of joints	536:585	It is ascertained that intra-articular maintenance of chondroitin sulfate (the preparation "mukosat") acts as a stimulus for reparative regeneration of connective tissue thus showing up positive changes in the status of connective tissue elements of joints: decrease in glycosaminoglycan content in blood serum and normalization of the composition of glycosaminoglycan carbohydrate component.
26226692	3	58	theme	glycosaminoglycans	795:812	arg1	biosynthesis					761:772	biosynthesis	761:772	biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints	761:845	It probably depends on stimulation of biosynthesis of autologous normal glycosaminoglycans in tissues of animal knee joints.
27109764	7	0	theme	basic	1330:1334	arg1	factor					1354:1359	basic fibroblast growth factor	1330:1359	basic fibroblast growth factor	1330:1359	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	10	1	theme	effective	2037:2045	arg1	agents					2059:2064	more effective therapeutic agents	2032:2064	more effective therapeutic agents	2032:2064	Our results indicate that our platform offers a simple and efficient means to isolate and enrich CSCs in vitro, which can help researchers better understand CSC biology and thus develop more effective therapeutic agents to treat cancer.
27109764	6	2	theme	CSC	1177:1179	arg1	proliferation					1181:1193	CSC proliferation	1177:1193	CSC proliferation	1177:1193	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	8	3	theme	increased	1516:1524	arg1	expression					1526:1535	increased expression	1516:1535	increased expression of the genes involved in breast CSC and NSC	1516:1579	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	1	4	theme	anti-cancer	220:230	arg1	therapies					232:240	novel anti-cancer therapies	214:240	novel anti-cancer therapies	214:240	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	7	5	theme	factor	1319:1324	arg1	immobilization					1361:1374	epidermal growth factor and basic fibroblast growth factor immobilization	1302:1374	epidermal growth factor and basic fibroblast growth factor immobilization	1302:1374	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	1	6	theme	primary	76:82	arg1	determinants					84:95	the primary determinants	72:95	the primary determinants of the clinical behaviors of human cancers	72:138	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	11	7	dep	STATEMENT	2083:2091	arg1	determinants					2124:2135	the primary determinants	2112:2135	the primary determinants of the clinical behaviors of human cancers	2112:2178	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	13	8	theme	Emerging	2427:2434	arg1	evidence					2436:2443	Emerging evidence	2427:2443	Emerging evidence	2427:2443	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	7	9	theme	epidermal	1302:1310	arg1	factor					1319:1324	epidermal growth factor	1302:1324	epidermal growth factor	1302:1324	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	4	10	theme	biology	747:753	arg1	discovery					730:738	the discovery	726:738	the discovery of CSC biology	726:753	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	11	11	theme	primary	2116:2122	arg1	determinants					2124:2135	the primary determinants	2112:2135	the primary determinants of the clinical behaviors of human cancers	2112:2178	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	6	12	theme	mechanical	1049:1058	arg1	properties					1060:1069	the material's mechanical properties	1034:1069	the material's mechanical properties	1034:1069	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	8	13	theme	genes	1544:1548	arg1	expression					1526:1535	increased expression	1516:1535	increased expression of the genes involved in breast CSC and NSC	1516:1579	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	1	14	theme	cancer	158:163	arg1	CSCs					177:180	CSCs	177:180	CSCs	177:180	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	1	14	theme	cancer	158:163	arg1	cells					170:174	cancer stem cells	158:174	cancer stem cells (CSCs)	158:181	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	7	15	theme	hyaluronic	1269:1278	arg1	HA					1286:1287	HA	1286:1287	HA	1286:1287	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	7	15	theme	hyaluronic	1269:1278	arg1	acid					1280:1283	hyaluronic acid	1269:1283	hyaluronic acid (HA)	1269:1288	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	6	16	theme	extracellular	1124:1136	arg1	matrix					1138:1143	the extracellular matrix	1120:1143	the extracellular matrix's (ECM's) molecular impact	1120:1170	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	1	17	theme	cells	170:174	arg1	discovery					145:153	the discovery	141:153	the discovery of cancer stem cells (CSCs)	141:181	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	11	18	theme	behaviors	2153:2161	arg1	determinants					2124:2135	the primary determinants	2112:2135	the primary determinants of the clinical behaviors of human cancers	2112:2178	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	8	19	contain	have	1662:1665	arg2	ability					1671:1677	the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo	1667:1748	the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo	1667:1748	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	8	19	contain	have	1662:1665	arg1	CSCs					1631:1634	4T1 CSCs	1627:1634	4T1 CSCs	1627:1634	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	15	20	theme	alginate-based	2796:2809	arg1	platform					2811:2818	a novel alginate-based platform	2788:2818	a novel alginate-based platform	2788:2818	In our study, a novel alginate-based platform were developed for mimicking the CSC niche to promote CSC proliferation and enrichment.
27109764	8	21	theme	subcutaneous	1697:1708	arg1	tumor					1710:1714	a subcutaneous tumor	1695:1714	a subcutaneous tumor in homologous Balb/c mice	1695:1740	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	7	22	theme	CSCs	1458:1461	arg1	niche					1464:1468	the 4T1 breast CSCs (4T1 CSCs) niche	1433:1468	the 4T1 breast CSCs (4T1 CSCs) niche	1433:1468	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	12	23	theme	therapeutic	2407:2417	arg1	agents					2419:2424	therapeutic agents	2407:2424	therapeutic agents	2407:2424	However, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	12	24	theme	biology	2376:2382	arg1	study					2363:2367	the study	2359:2367	the study of CSC biology	2359:2382	However, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	12	24	theme	biology	2376:2382	arg1	development					2392:2402	the development	2388:2402	the development of therapeutic agents	2388:2424	However, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	4	25	theme	three-dimensional	624:640	arg1	scaffolds					654:662	three-dimensional (3D) porous scaffolds	624:662	three-dimensional (3D) porous scaffolds	624:662	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	14	26	theme	in	2598:2599	arg1	microenvironment					2610:2625	the in vivo CSC microenvironment	2594:2625	the in vivo CSC microenvironment	2594:2625	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	1	27	theme	behaviors	113:121	arg1	determinants					84:95	the primary determinants	72:95	the primary determinants of the clinical behaviors of human cancers	72:138	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	5	28	dep	CSC	860:862	arg1	proliferation					864:876	proliferation	864:876	proliferation	864:876	This paper presents our study on a novel alginate-based platform for mimicking the CSC niche to promote CSC proliferation and enrichment.
27109764	8	29	theme	Balb/c	1730:1735	arg1	mice					1737:1740	homologous Balb/c mice	1719:1740	homologous Balb/c mice	1719:1740	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	1	30	theme	human	126:130	arg1	cancers					132:138	human cancers	126:138	human cancers	126:138	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	7	31	theme	breast	1441:1446	arg1	niche					1464:1468	the 4T1 breast CSCs (4T1 CSCs) niche	1433:1468	the 4T1 breast CSCs (4T1 CSCs) niche	1433:1468	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	3	32	theme	normal	538:543	arg1	NSCs					557:560	NSCs	557:560	NSCs	557:560	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	3	32	theme	normal	538:543	arg1	cells					550:554	normal stem cells	538:554	normal stem cells (NSCs)	538:561	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	8	33	theme	2D	1605:1606	arg1	culture					1614:1620	general 2D or 3D culture	1597:1620	general 2D or 3D culture	1597:1620	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	6	34	theme	impact	1165:1170	arg1	composition					1105:1115	the composition	1101:1115	the composition of the extracellular matrix's (ECM's) molecular impact	1101:1170	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	14	35	theme	CSC	2761:2763	arg1	biology					2765:2771	CSC biology	2761:2771	CSC biology	2761:2771	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	8	36	theme	3D	1611:1612	arg1	culture					1614:1620	general 2D or 3D culture	1597:1620	general 2D or 3D culture	1597:1620	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	5	37	theme	alginate-based	797:810	arg1	platform					812:819	a novel alginate-based platform	789:819	a novel alginate-based platform for mimicking the CSC niche to promote CSC proliferation and enrichment	789:891	This paper presents our study on a novel alginate-based platform for mimicking the CSC niche to promote CSC proliferation and enrichment.
27109764	11	38	theme	clinical	2144:2151	arg1	behaviors					2153:2161	the clinical behaviors	2140:2161	the clinical behaviors of human cancers	2140:2178	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	11	39	theme	novel	2254:2258	arg1	therapies					2272:2280	novel anti-cancer therapies	2254:2280	novel anti-cancer therapies	2254:2280	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	3	40	with	case	528:531	arg1	NSCs					557:560	NSCs	557:560	NSCs	557:560	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	3	40	with	case	528:531	arg1	cells					550:554	normal stem cells	538:554	normal stem cells (NSCs)	538:561	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	8	41	theme	4T1	1627:1629	arg1	CSCs					1631:1634	4T1 CSCs	1627:1634	4T1 CSCs	1627:1634	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	9	42	theme	different	1806:1814	arg1	parameters					1816:1825	different parameters	1806:1825	different parameters for CSC screening	1806:1843	In addition, the platform can be adjusted according to different parameters for CSC screening.
27109764	4	43	theme	porous	647:652	arg1	scaffolds					654:662	three-dimensional (3D) porous scaffolds	624:662	three-dimensional (3D) porous scaffolds	624:662	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	6	44	theme	cancer	946:951	arg1	model					953:957	a versatile mouse 4T1 breast cancer model	917:957	a versatile mouse 4T1 breast cancer model	917:957	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	2	45	theme	CSC	354:356	arg1	biology					358:364	CSC biology	354:364	CSC biology	354:364	Notably, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	13	46	theme	normal	2556:2561	arg1	NSCs					2575:2578	NSCs	2575:2578	NSCs	2575:2578	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	13	46	theme	normal	2556:2561	arg1	cells					2568:2572	normal stem cells	2556:2572	normal stem cells (NSCs)	2556:2579	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	13	47	theme	stem	2563:2566	arg1	NSCs					2575:2578	NSCs	2575:2578	NSCs	2575:2578	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	13	47	theme	stem	2563:2566	arg1	cells					2568:2572	normal stem cells	2556:2572	normal stem cells (NSCs)	2556:2579	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	6	48	theme	4T1	935:937	arg1	model					953:957	a versatile mouse 4T1 breast cancer model	917:957	a versatile mouse 4T1 breast cancer model	917:957	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	0	49	theme	cancer	31:36	arg1	research					48:55	cancer stem cell research	31:55	cancer stem cell research	31:55	An alginate-based platform for cancer stem cell research.
27109764	14	50	dep	in	2598:2599	arg1	vivo					2601:2604	vivo	2601:2604	vivo	2601:2604	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	6	51	theme	versatile	919:927	arg1	model					953:957	a versatile mouse 4T1 breast cancer model	917:957	a versatile mouse 4T1 breast cancer model	917:957	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	2	52	theme	therapeutic	389:399	arg1	agents					401:406	therapeutic agents	389:406	therapeutic agents	389:406	Notably, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	0	53	theme	cell	43:46	arg1	research					48:55	cancer stem cell research	31:55	cancer stem cell research	31:55	An alginate-based platform for cancer stem cell research.
27109764	7	54	theme	factor	1354:1359	arg1	immobilization					1361:1374	epidermal growth factor and basic fibroblast growth factor immobilization	1302:1374	epidermal growth factor and basic fibroblast growth factor immobilization	1302:1374	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	6	55	theme	CSC	1012:1014	arg1	niche					1016:1020	a CSC niche	1010:1020	a CSC niche	1010:1020	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	3	56	theme	Emerging	409:416	arg1	evidence					418:425	Emerging evidence	409:425	Emerging evidence	409:425	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	8	57	theme	4T1	1475:1477	arg1	CSCs					1479:1482	The 4T1 CSCs	1471:1482	The 4T1 CSCs obtained from the platform	1471:1509	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	7	58	theme	fibroblast	1336:1345	arg1	factor					1354:1359	basic fibroblast growth factor	1330:1359	basic fibroblast growth factor	1330:1359	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	4	59	dep	in	580:581	arg1	vivo					583:586	vivo	583:586	vivo	583:586	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	6	60	theme	matrix	989:994	arg1	parameters					996:1005	the matrix parameters	985:1005	the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment	985:1208	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	6	61	theme	molecular	1155:1163	arg1	impact					1165:1170	the extracellular matrix's (ECM's) molecular impact	1120:1170	the extracellular matrix's (ECM's) molecular impact	1120:1170	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	6	61	theme	molecular	1155:1163	arg1	ECM					1148:1150	ECM's	1148:1152	ECM's	1148:1152	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	1	62	theme	novel	214:218	arg1	therapies					232:240	novel anti-cancer therapies	214:240	novel anti-cancer therapies	214:240	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	7	63	theme	growth	1312:1317	arg1	factor					1319:1324	epidermal growth factor	1302:1324	epidermal growth factor	1302:1324	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	14	64	theme	three-dimensional	2642:2658	arg1	scaffolds					2672:2680	three-dimensional (3D) porous scaffolds	2642:2680	three-dimensional (3D) porous scaffolds	2642:2680	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	11	65	theme	SIGNIFICANCE	2096:2107	arg1	STATEMENT					2083:2091	STATEMENT	2083:2091	STATEMENT OF SIGNIFICANCE	2083:2107	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	14	66	theme	porous	2665:2670	arg1	scaffolds					2672:2680	three-dimensional (3D) porous scaffolds	2642:2680	three-dimensional (3D) porous scaffolds	2642:2680	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	10	67	theme	in	1948:1949	arg1	CSCs					1943:1946	CSCs	1943:1946	CSCs	1943:1946	Our results indicate that our platform offers a simple and efficient means to isolate and enrich CSCs in vitro, which can help researchers better understand CSC biology and thus develop more effective therapeutic agents to treat cancer.
27109764	10	68	theme	simple	1894:1899	arg1	means					1915:1919	a simple and efficient means	1892:1919	a simple and efficient means to isolate and enrich CSCs in vitro, which can help researchers better understand CSC biology and thus develop more effective therapeutic agents to treat cancer	1892:2080	Our results indicate that our platform offers a simple and efficient means to isolate and enrich CSCs in vitro, which can help researchers better understand CSC biology and thus develop more effective therapeutic agents to treat cancer.
27109764	4	69	theme	CSC	743:745	arg1	biology					747:753	CSC biology	743:753	CSC biology	743:753	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	6	70	used	used	912:915	arg2	we					909:910	we	909:910	we	909:910	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	1	71	theme	stem	165:168	arg1	CSCs					177:180	CSCs	177:180	CSCs	177:180	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	1	71	theme	stem	165:168	arg1	cells					170:174	cancer stem cells	158:174	cancer stem cells (CSCs)	158:181	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	10	72	theme	therapeutic	2047:2057	arg1	agents					2059:2064	more effective therapeutic agents	2032:2064	more effective therapeutic agents	2032:2064	Our results indicate that our platform offers a simple and efficient means to isolate and enrich CSCs in vitro, which can help researchers better understand CSC biology and thus develop more effective therapeutic agents to treat cancer.
27109764	7	73	theme	acid	1280:1283	arg1	concentration					1252:1264	concentration	1252:1264	concentration	1252:1264	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	7	73	theme	acid	1280:1283	arg1	stiffness					1238:1246	the optimal stiffness	1226:1246	the optimal stiffness	1226:1246	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	4	74	theme	in	580:581	arg1	microenvironment					592:607	the in vivo CSC microenvironment	576:607	the in vivo CSC microenvironment	576:607	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	15	75	theme	novel	2790:2794	arg1	platform					2811:2818	a novel alginate-based platform	2788:2818	a novel alginate-based platform	2788:2818	In our study, a novel alginate-based platform were developed for mimicking the CSC niche to promote CSC proliferation and enrichment.
27109764	10	76	theme	efficient	1905:1913	arg1	means					1915:1919	a simple and efficient means	1892:1919	a simple and efficient means to isolate and enrich CSCs in vitro, which can help researchers better understand CSC biology and thus develop more effective therapeutic agents to treat cancer	1892:2080	Our results indicate that our platform offers a simple and efficient means to isolate and enrich CSCs in vitro, which can help researchers better understand CSC biology and thus develop more effective therapeutic agents to treat cancer.
27109764	8	77	theme	breast	1562:1567	arg1	CSC					1569:1571	breast CSC	1562:1571	breast CSC	1562:1571	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	15	78	theme	CSC	2874:2876	arg1	proliferation					2878:2890	CSC proliferation	2874:2890	CSC proliferation	2874:2890	In our study, a novel alginate-based platform were developed for mimicking the CSC niche to promote CSC proliferation and enrichment.
27109764	7	79	theme	4T1	1454:1456	arg1	niche					1464:1468	the 4T1 breast CSCs (4T1 CSCs) niche	1433:1468	the 4T1 breast CSCs (4T1 CSCs) niche	1433:1468	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	11	80	theme	anti-cancer	2260:2270	arg1	therapies					2272:2280	novel anti-cancer therapies	2254:2280	novel anti-cancer therapies	2254:2280	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	13	81	with	case	2546:2549	arg1	NSCs					2575:2578	NSCs	2575:2578	NSCs	2575:2578	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	13	81	with	case	2546:2549	arg1	cells					2568:2572	normal stem cells	2556:2572	normal stem cells (NSCs)	2556:2579	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	8	82	theme	homologous	1719:1728	arg1	mice					1737:1740	homologous Balb/c mice	1719:1740	homologous Balb/c mice	1719:1740	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	1	83	theme	clinical	104:111	arg1	behaviors					113:121	the clinical behaviors	100:121	the clinical behaviors of human cancers	100:138	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	7	84	theme	CSCs	1448:1451	arg1	niche					1464:1468	the 4T1 breast CSCs (4T1 CSCs) niche	1433:1468	the 4T1 breast CSCs (4T1 CSCs) niche	1433:1468	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	7	85	theme	optimal	1230:1236	arg1	stiffness					1238:1246	the optimal stiffness	1226:1246	the optimal stiffness	1226:1246	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	11	86	theme	cancers	2172:2178	arg1	behaviors					2153:2161	the clinical behaviors	2140:2161	the clinical behaviors of human cancers	2140:2178	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	3	87	theme	stem	545:548	arg1	NSCs					557:560	NSCs	557:560	NSCs	557:560	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	3	87	theme	stem	545:548	arg1	cells					550:554	normal stem cells	538:554	normal stem cells (NSCs)	538:561	Emerging evidence indicates that CSCs rely on a niche that controls their differentiation and proliferation, as is the case with normal stem cells (NSCs).
27109764	7	88	theme	4T1	1437:1439	arg1	niche					1464:1468	the 4T1 breast CSCs (4T1 CSCs) niche	1433:1468	the 4T1 breast CSCs (4T1 CSCs) niche	1433:1468	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	12	89	theme	agents	2419:2424	arg1	study					2363:2367	the study	2359:2367	the study of CSC biology	2359:2382	However, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	12	89	theme	agents	2419:2424	arg1	development					2392:2402	the development	2388:2402	the development of therapeutic agents	2388:2424	However, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	11	90	theme	cells	2210:2214	arg1	discovery					2185:2193	the discovery	2181:2193	the discovery of cancer stem cells (CSCs)	2181:2221	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	5	91	from	study	780:784	arg1	platform					812:819	a novel alginate-based platform	789:819	a novel alginate-based platform for mimicking the CSC niche to promote CSC proliferation and enrichment	789:891	This paper presents our study on a novel alginate-based platform for mimicking the CSC niche to promote CSC proliferation and enrichment.
27109764	1	92	theme	cancers	132:138	arg1	behaviors					113:121	the clinical behaviors	100:121	the clinical behaviors of human cancers	100:138	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	15	93	theme	CSC	2853:2855	arg1	niche					2857:2861	the CSC niche	2849:2861	the CSC niche to promote CSC proliferation and enrichment	2849:2905	In our study, a novel alginate-based platform were developed for mimicking the CSC niche to promote CSC proliferation and enrichment.
27109764	6	94	theme	cytokine	1072:1079	arg1	immobilization					1081:1094	cytokine immobilization	1072:1094	cytokine immobilization	1072:1094	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	11	95	theme	cancer	2198:2203	arg1	CSCs					2217:2220	CSCs	2217:2220	CSCs	2217:2220	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	11	95	theme	cancer	2198:2203	arg1	cells					2210:2214	cancer stem cells	2198:2214	cancer stem cells (CSCs)	2198:2221	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	4	96	theme	CSC	588:590	arg1	microenvironment					592:607	the in vivo CSC microenvironment	576:607	the in vivo CSC microenvironment	576:607	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	14	97	theme	biology	2765:2771	arg1	discovery					2748:2756	the discovery	2744:2756	the discovery of CSC biology	2744:2771	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	14	98	dep	three-dimensional	2642:2658	arg1	3D					2661:2662	3D	2661:2662	3D	2661:2662	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	8	99	theme	general	1597:1603	arg1	culture					1614:1620	general 2D or 3D culture	1597:1620	general 2D or 3D culture	1597:1620	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	5	100	theme	novel	791:795	arg1	platform					812:819	a novel alginate-based platform	789:819	a novel alginate-based platform for mimicking the CSC niche to promote CSC proliferation and enrichment	789:891	This paper presents our study on a novel alginate-based platform for mimicking the CSC niche to promote CSC proliferation and enrichment.
27109764	1	101	dep	Kievit	243:248	arg1	al.					253:255	Kievit et al.	243:255	Kievit et al.	243:255	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	12	102	theme	CSC	2372:2374	arg1	biology					2376:2382	CSC biology	2372:2382	CSC biology	2372:2382	However, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	10	103	dep	in	1948:1949	arg1	vitro					1951:1955	vitro	1951:1955	vitro	1951:1955	Our results indicate that our platform offers a simple and efficient means to isolate and enrich CSCs in vitro, which can help researchers better understand CSC biology and thus develop more effective therapeutic agents to treat cancer.
27109764	11	104	theme	ideal	2237:2241	arg1	target					2243:2248	an ideal target	2234:2248	an ideal target for novel anti-cancer therapies	2234:2280	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	11	105	theme	human	2166:2170	arg1	cancers					2172:2178	human cancers	2166:2178	human cancers	2166:2178	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	0	106	theme	alginate-based	3:16	arg1	platform					18:25	An alginate-based platform	0:25	An alginate-based platform for cancer stem cell research	0:55	An alginate-based platform for cancer stem cell research.
27109764	5	107	theme	CSC	839:841	arg1	niche					843:847	the CSC niche	835:847	the CSC niche to promote CSC proliferation and enrichment	835:891	This paper presents our study on a novel alginate-based platform for mimicking the CSC niche to promote CSC proliferation and enrichment.
27109764	10	108	theme	CSC	2003:2005	arg1	biology					2007:2013	CSC biology	2003:2013	CSC biology	2003:2013	Our results indicate that our platform offers a simple and efficient means to isolate and enrich CSCs in vitro, which can help researchers better understand CSC biology and thus develop more effective therapeutic agents to treat cancer.
27109764	4	109	dep	three-dimensional	624:640	arg1	3D					643:644	3D	643:644	3D	643:644	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	6	110	theme	breast	939:944	arg1	model					953:957	a versatile mouse 4T1 breast cancer model	917:957	a versatile mouse 4T1 breast cancer model	917:957	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	2	111	theme	biology	358:364	arg1	study					345:349	the study	341:349	the study of CSC biology	341:364	Notably, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	2	111	theme	biology	358:364	arg1	development					374:384	the development	370:384	the development of therapeutic agents	370:406	Notably, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	9	112	theme	CSC	1831:1833	arg1	screening					1835:1843	CSC screening	1831:1843	CSC screening	1831:1843	In addition, the platform can be adjusted according to different parameters for CSC screening.
27109764	11	113	theme	stem	2205:2208	arg1	CSCs					2217:2220	CSCs	2217:2220	CSCs	2217:2220	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	11	113	theme	stem	2205:2208	arg1	cells					2210:2214	cancer stem cells	2198:2214	cancer stem cells (CSCs)	2198:2221	STATEMENT OF SIGNIFICANCE As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies.
27109764	0	114	theme	stem	38:41	arg1	research					48:55	cancer stem cell research	31:55	cancer stem cell research	31:55	An alginate-based platform for cancer stem cell research.
27109764	6	115	theme	mouse	929:933	arg1	model					953:957	a versatile mouse 4T1 breast cancer model	917:957	a versatile mouse 4T1 breast cancer model	917:957	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	2	116	theme	agents	401:406	arg1	study					345:349	the study	341:349	the study of CSC biology	341:364	Notably, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	2	116	theme	agents	401:406	arg1	development					374:384	the development	370:384	the development of therapeutic agents	370:406	Notably, CSCs are difficult to propagate in vitro, which severely restricts the study of CSC biology and the development of therapeutic agents.
27109764	6	117	theme	niche	1016:1020	arg1	parameters					996:1005	the matrix parameters	985:1005	the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment	985:1208	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	14	118	theme	CSC	2606:2608	arg1	microenvironment					2610:2625	the in vivo CSC microenvironment	2594:2625	the in vivo CSC microenvironment	2594:2625	Replicating the in vivo CSC microenvironment in vitro using three-dimensional (3D) porous scaffolds can provide means to effectively generate CSCs, thus enabling the discovery of CSC biology.
27109764	7	119	theme	immobilization	1361:1374	arg1	concentration					1252:1264	concentration	1252:1264	concentration	1252:1264	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	7	119	theme	immobilization	1361:1374	arg1	stiffness					1238:1246	the optimal stiffness	1226:1246	the optimal stiffness	1226:1246	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	8	120	from	tumor	1710:1714	arg1	mice					1737:1740	homologous Balb/c mice	1719:1740	homologous Balb/c mice	1719:1740	The 4T1 CSCs obtained from the platform show increased expression of the genes involved in breast CSC and NSC, as compared to general 2D or 3D culture, and 4T1 CSCs were also demonstrated to have the ability to quickly form a subcutaneous tumor in homologous Balb/c mice in vivo.
27109764	1	121	dep	represents	183:192	arg1	Kievit					243:248	Kievit	243:248	Kievit	243:248	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	1	122	theme	ideal	197:201	arg1	target					203:208	an ideal target	194:208	an ideal target for novel anti-cancer therapies	194:240	UNLABELLED As the primary determinants of the clinical behaviors of human cancers, the discovery of cancer stem cells (CSCs) represents an ideal target for novel anti-cancer therapies (Kievit et al., 2014).
27109764	7	123	theme	growth	1347:1352	arg1	factor					1354:1359	basic fibroblast growth factor	1330:1359	basic fibroblast growth factor	1330:1359	On this basis, the optimal stiffness and concentration of hyaluronic acid (HA), as well as epidermal growth factor and basic fibroblast growth factor immobilization, were identified to establish the platform for mimicking the 4T1 breast CSCs (4T1 CSCs) niche.
27109764	6	124	from	parameters	996:1005	arg1	enrichment					1199:1208	enrichment	1199:1208	enrichment	1199:1208	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
27109764	6	124	from	parameters	996:1005	arg1	proliferation					1181:1193	CSC proliferation	1177:1193	CSC proliferation	1177:1193	In this study, we used a versatile mouse 4T1 breast cancer model to independently evaluate the matrix parameters of a CSC niche - including the material's mechanical properties, cytokine immobilization, and the composition of the extracellular matrix's (ECM's) molecular impact - on CSC proliferation and enrichment.
24680284	8	0	theme	bone	1271:1274	arg1	thickness					1342:1350	cortical thickness	1333:1350	cortical thickness	1333:1350	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	0	theme	bone	1271:1274	arg1	properties					1257:1266	several compositional properties	1235:1266	several compositional properties	1235:1266	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	0	theme	bone	1271:1274	arg1	volume					1373:1378	trabecular bone volume	1357:1378	trabecular bone volume	1357:1378	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	0	theme	bone	1271:1274	arg1	matrix					1302:1307	increased bone matrix	1287:1307	increased bone matrix	1287:1307	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	0	theme	bone	1271:1274	arg1	concentration					1318:1330	mineral concentration	1310:1330	mineral concentration	1310:1330	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	2	1	theme	genes	325:329	arg1	expression					291:300	the expression	287:300	the expression of osteoblast specific genes at 1 and 2 weeks after surgery	287:360	Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery.
24680284	8	2	theme	compositional	1243:1255	arg1	thickness					1342:1350	cortical thickness	1333:1350	cortical thickness	1333:1350	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	2	theme	compositional	1243:1255	arg1	properties					1257:1266	several compositional properties	1235:1266	several compositional properties	1235:1266	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	2	theme	compositional	1243:1255	arg1	volume					1373:1378	trabecular bone volume	1357:1378	trabecular bone volume	1357:1378	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	2	theme	compositional	1243:1255	arg1	matrix					1302:1307	increased bone matrix	1287:1307	increased bone matrix	1287:1307	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	2	theme	compositional	1243:1255	arg1	concentration					1318:1330	mineral concentration	1310:1330	mineral concentration	1310:1330	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	6	3	theme	Immunohistochemical	877:895	arg1	examination					897:907	Immunohistochemical examination	877:907	Immunohistochemical examination	877:907	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	2	4	theme	specific	316:323	arg1	genes					325:329	osteoblast specific genes	305:329	osteoblast specific genes	305:329	Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery.
24680284	6	5	theme	genes	983:987	arg1	genes					983:987	osteoblast specific genes	963:987	osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	963:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	5	theme	genes	983:987	arg1	receptor					1012:1019	TGF-β type III receptor	997:1019	TGF-β type III receptor	997:1019	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	5	theme	genes	983:987	arg1	Runx2					1022:1026	Runx2	1022:1026	Runx2	1022:1026	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	5	theme	genes	983:987	arg1	set					956:958	a set	954:958	a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	954:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	5	theme	genes	983:987	arg1	osteocalcin					1049:1059	osteocalcin	1049:1059	osteocalcin	1049:1059	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	5	theme	genes	983:987	arg1	TGF-β					990:994	TGF-β	990:994	TGF-β	990:994	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	5	theme	genes	983:987	arg1	collagen					1036:1043	type I collagen	1029:1043	type I collagen	1029:1043	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	8	6	theme	mineral	1310:1316	arg1	concentration					1318:1330	mineral concentration	1310:1330	mineral concentration	1310:1330	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	6	7	dep	genes	983:987	arg1	genes					983:987	osteoblast specific genes	963:987	osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	963:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	7	dep	genes	983:987	arg1	receptor					1012:1019	TGF-β type III receptor	997:1019	TGF-β type III receptor	997:1019	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	7	dep	genes	983:987	arg1	Runx2					1022:1026	Runx2	1022:1026	Runx2	1022:1026	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	7	dep	genes	983:987	arg1	osteocalcin					1049:1059	osteocalcin	1049:1059	osteocalcin	1049:1059	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	7	dep	genes	983:987	arg1	TGF-β					990:994	TGF-β	990:994	TGF-β	990:994	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	7	dep	genes	983:987	arg1	collagen					1036:1043	type I collagen	1029:1043	type I collagen	1029:1043	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	8	8	theme	trabecular	1357:1366	arg1	volume					1373:1378	trabecular bone volume	1357:1378	trabecular bone volume	1357:1378	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	4	9	theme	hydrophobic	718:728	arg1	nature					730:735	the hydrophobic nature	714:735	the hydrophobic nature into hydrophilic	714:752	The contact angle measurements confirmed the introduction of plasma helped to change the hydrophobic nature into hydrophilic.
24680284	4	10	dep	confirmed	660:668	arg1	helped					697:702	helped	697:702	confirmed the introduction of plasma helped to change the hydrophobic nature into hydrophilic	660:752	The contact angle measurements confirmed the introduction of plasma helped to change the hydrophobic nature into hydrophilic.
24680284	6	11	theme	specific	974:981	arg1	genes					983:987	osteoblast specific genes	963:987	osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	963:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	11	theme	specific	974:981	arg1	receptor					1012:1019	TGF-β type III receptor	997:1019	TGF-β type III receptor	997:1019	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	11	theme	specific	974:981	arg1	Runx2					1022:1026	Runx2	1022:1026	Runx2	1022:1026	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	11	theme	specific	974:981	arg1	osteocalcin					1049:1059	osteocalcin	1049:1059	osteocalcin	1049:1059	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	11	theme	specific	974:981	arg1	TGF-β					990:994	TGF-β	990:994	TGF-β	990:994	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	11	theme	specific	974:981	arg1	collagen					1036:1043	type I collagen	1029:1043	type I collagen	1029:1043	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	12	theme	osteoblast	963:972	arg1	genes					983:987	osteoblast specific genes	963:987	osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	963:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	12	theme	osteoblast	963:972	arg1	receptor					1012:1019	TGF-β type III receptor	997:1019	TGF-β type III receptor	997:1019	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	12	theme	osteoblast	963:972	arg1	Runx2					1022:1026	Runx2	1022:1026	Runx2	1022:1026	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	12	theme	osteoblast	963:972	arg1	osteocalcin					1049:1059	osteocalcin	1049:1059	osteocalcin	1049:1059	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	12	theme	osteoblast	963:972	arg1	TGF-β					990:994	TGF-β	990:994	TGF-β	990:994	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	12	theme	osteoblast	963:972	arg1	collagen					1036:1043	type I collagen	1029:1043	type I collagen	1029:1043	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	5	13	attach	presence	795:802	arg1	bone					850:853	newly formed bone	837:853	newly formed bone	837:853	The histological analyses confirmed the presence of silk fibroin in scaffolds and newly formed bone around the scaffolds.
24680284	5	13	attach	presence	795:802	arg1	scaffolds					823:831	scaffolds	823:831	scaffolds	823:831	The histological analyses confirmed the presence of silk fibroin in scaffolds and newly formed bone around the scaffolds.
24680284	5	13	attach	presence	795:802	arg2	fibroin					812:818	silk fibroin	807:818	silk fibroin	807:818	The histological analyses confirmed the presence of silk fibroin in scaffolds and newly formed bone around the scaffolds.
24680284	3	14	theme	aqueous-based	501:513	arg1	scaffold					534:541	the aqueous-based silk fibroin (A-F) scaffold	497:541	the aqueous-based silk fibroin (A-F) scaffold	497:541	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	3	15	theme	scanning	433:440	arg1	microcopy					453:461	the scanning electronic microcopy	429:461	the scanning electronic microcopy	429:461	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	4	16	theme	angle	641:645	arg1	measurements					647:658	The contact angle measurements	629:658	The contact angle measurements	629:658	The contact angle measurements confirmed the introduction of plasma helped to change the hydrophobic nature into hydrophilic.
24680284	3	17	theme	electronic	442:451	arg1	microcopy					453:461	the scanning electronic microcopy	429:461	the scanning electronic microcopy	429:461	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	5	18	theme	silk	807:810	arg1	fibroin					812:818	silk fibroin	807:818	silk fibroin	807:818	The histological analyses confirmed the presence of silk fibroin in scaffolds and newly formed bone around the scaffolds.
24680284	8	19	theme	bone	1297:1300	arg1	matrix					1302:1307	increased bone matrix	1287:1307	increased bone matrix	1287:1307	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	6	20	theme	type	1029:1032	arg1	genes					983:987	osteoblast specific genes	963:987	osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	963:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	20	theme	type	1029:1032	arg1	collagen					1036:1043	type I collagen	1029:1043	type I collagen	1029:1043	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	1	21	theme	bone	151:154	arg1	properties					163:172	bone matrix properties	151:172	bone matrix properties	151:172	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	5	22	theme	histological	759:770	arg1	analyses					772:779	The histological analyses	755:779	The histological analyses	755:779	The histological analyses confirmed the presence of silk fibroin in scaffolds and newly formed bone around the scaffolds.
24680284	8	23	theme	increased	1287:1295	arg1	matrix					1302:1307	increased bone matrix	1287:1307	increased bone matrix	1287:1307	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	1	24	theme	matrix	156:161	arg1	properties					163:172	bone matrix properties	151:172	bone matrix properties	151:172	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	3	25	theme	sized	482:486	arg1	pores					488:492	the large sized pores	472:492	the large sized pores	472:492	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	5	26	from	presence	795:802	arg1	bone					850:853	newly formed bone	837:853	newly formed bone	837:853	The histological analyses confirmed the presence of silk fibroin in scaffolds and newly formed bone around the scaffolds.
24680284	5	26	from	presence	795:802	arg1	scaffolds					823:831	scaffolds	823:831	scaffolds	823:831	The histological analyses confirmed the presence of silk fibroin in scaffolds and newly formed bone around the scaffolds.
24680284	1	27	theme	properties	163:172	arg1	regulator					138:146	a key regulator	132:146	a key regulator of bone matrix properties and composition	132:188	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	1	27	theme	properties	163:172	arg1	fibroin					114:120	plasma-irradiated silk fibroin	91:120	plasma-irradiated silk fibroin (P-AF)	91:127	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	7	28	theme	bone	1120:1123	arg1	matrix					1125:1130	bone matrix	1120:1130	bone matrix	1120:1130	These data were the first to show that the properties of bone matrix are regulated, specifically through Runx2 pathway in P-AF group.
24680284	7	29	from	pathway	1174:1180	arg1	group					1190:1194	P-AF group	1185:1194	P-AF group	1185:1194	These data were the first to show that the properties of bone matrix are regulated, specifically through Runx2 pathway in P-AF group.
24680284	1	30	theme	plasma-irradiated	91:107	arg1	regulator					138:146	a key regulator	132:146	a key regulator of bone matrix properties and composition	132:188	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	1	30	theme	plasma-irradiated	91:107	arg1	P-AF					123:126	P-AF	123:126	P-AF	123:126	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	1	30	theme	plasma-irradiated	91:107	arg1	fibroin					114:120	plasma-irradiated silk fibroin	91:120	plasma-irradiated silk fibroin (P-AF)	91:127	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	5	31	theme	formed	843:848	arg1	bone					850:853	newly formed bone	837:853	newly formed bone	837:853	The histological analyses confirmed the presence of silk fibroin in scaffolds and newly formed bone around the scaffolds.
24680284	0	32	theme	silk	28:31	arg1	Effect					0:5	Effect	0:5	Effect of plasma-irradiated silk	0:31	Effect of plasma-irradiated silk fibroin in bone regeneration.
24680284	4	33	theme	plasma	690:695	arg1	introduction					674:685	the introduction	670:685	the introduction of plasma	670:695	The contact angle measurements confirmed the introduction of plasma helped to change the hydrophobic nature into hydrophilic.
24680284	3	34	theme	silk	515:518	arg1	scaffold					534:541	the aqueous-based silk fibroin (A-F) scaffold	497:541	the aqueous-based silk fibroin (A-F) scaffold	497:541	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	1	35	theme	composition	178:188	arg1	regulator					138:146	a key regulator	132:146	a key regulator of bone matrix properties and composition	132:188	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	1	35	theme	composition	178:188	arg1	fibroin					114:120	plasma-irradiated silk fibroin	91:120	plasma-irradiated silk fibroin (P-AF)	91:127	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	0	36	theme	plasma-irradiated	10:26	arg1	silk					28:31	plasma-irradiated silk	10:31	plasma-irradiated silk	10:31	Effect of plasma-irradiated silk fibroin in bone regeneration.
24680284	3	37	theme	fibroin	520:526	arg1	scaffold					534:541	the aqueous-based silk fibroin (A-F) scaffold	497:541	the aqueous-based silk fibroin (A-F) scaffold	497:541	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	4	38	theme	contact	633:639	arg1	measurements					647:658	The contact angle measurements	629:658	The contact angle measurements	629:658	The contact angle measurements confirmed the introduction of plasma helped to change the hydrophobic nature into hydrophilic.
24680284	5	39	theme	fibroin	812:818	arg1	presence					795:802	the presence	791:802	the presence of silk fibroin in scaffolds and newly formed bone	791:853	The histological analyses confirmed the presence of silk fibroin in scaffolds and newly formed bone around the scaffolds.
24680284	8	40	theme	cortical	1333:1340	arg1	thickness					1342:1350	cortical thickness	1333:1350	cortical thickness	1333:1350	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	3	41	theme	A-F	529:531	arg1	scaffold					534:541	the aqueous-based silk fibroin (A-F) scaffold	497:541	the aqueous-based silk fibroin (A-F) scaffold	497:541	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	7	42	theme	P-AF	1185:1188	arg1	group					1190:1194	P-AF group	1185:1194	P-AF group	1185:1194	These data were the first to show that the properties of bone matrix are regulated, specifically through Runx2 pathway in P-AF group.
24680284	8	43	theme	P-AF	1220:1223	arg1	employment					1206:1215	an employment	1203:1215	an employment of P-AF	1203:1223	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	6	44	theme	expression	932:941	arg1	pattern					943:949	the increased expression pattern	918:949	the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	918:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	2	45	theme	osteoblast	305:314	arg1	genes					325:329	osteoblast specific genes	305:329	osteoblast specific genes	305:329	Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery.
24680284	0	46	theme	bone	44:47	arg1	regeneration					49:60	bone regeneration	44:60	bone regeneration	44:60	Effect of plasma-irradiated silk fibroin in bone regeneration.
24680284	6	47	theme	increased	922:930	arg1	pattern					943:949	the increased expression pattern	918:949	the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	918:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	2	48	theme	matrix	196:201	arg1	properties					203:212	Bone matrix properties	191:212	Bone matrix properties	191:212	Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery.
24680284	6	49	from	pattern	943:949	arg1	genes					983:987	osteoblast specific genes	963:987	osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	963:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	49	from	pattern	943:949	arg1	receptor					1012:1019	TGF-β type III receptor	997:1019	TGF-β type III receptor	997:1019	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	49	from	pattern	943:949	arg1	Runx2					1022:1026	Runx2	1022:1026	Runx2	1022:1026	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	49	from	pattern	943:949	arg1	set					956:958	a set	954:958	a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	954:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	49	from	pattern	943:949	arg1	osteocalcin					1049:1059	osteocalcin	1049:1059	osteocalcin	1049:1059	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	49	from	pattern	943:949	arg1	TGF-β					990:994	TGF-β	990:994	TGF-β	990:994	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	49	from	pattern	943:949	arg1	collagen					1036:1043	type I collagen	1029:1043	type I collagen	1029:1043	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	2	50	theme	Bone	191:194	arg1	properties					203:212	Bone matrix properties	191:212	Bone matrix properties	191:212	Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery.
24680284	2	51	theme	size	247:250	arg1	defects					252:258	48 femur critical size defects	229:258	48 femur critical size defects (3.25 mm in diameter)	229:280	Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery.
24680284	2	52	from	weeks	342:346	arg1	expression					291:300	the expression	287:300	the expression of osteoblast specific genes at 1 and 2 weeks after surgery	287:360	Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery.
24680284	8	53	theme	several	1235:1241	arg1	thickness					1342:1350	cortical thickness	1333:1350	cortical thickness	1333:1350	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	53	theme	several	1235:1241	arg1	properties					1257:1266	several compositional properties	1235:1266	several compositional properties	1235:1266	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	53	theme	several	1235:1241	arg1	volume					1373:1378	trabecular bone volume	1357:1378	trabecular bone volume	1357:1378	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	53	theme	several	1235:1241	arg1	matrix					1302:1307	increased bone matrix	1287:1307	increased bone matrix	1287:1307	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	8	53	theme	several	1235:1241	arg1	concentration					1318:1330	mineral concentration	1310:1330	mineral concentration	1310:1330	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	1	54	theme	silk	109:112	arg1	regulator					138:146	a key regulator	132:146	a key regulator of bone matrix properties and composition	132:188	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	1	54	theme	silk	109:112	arg1	P-AF					123:126	P-AF	123:126	P-AF	123:126	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	1	54	theme	silk	109:112	arg1	fibroin					114:120	plasma-irradiated silk fibroin	91:120	plasma-irradiated silk fibroin (P-AF)	91:127	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	6	55	theme	type	1003:1006	arg1	genes					983:987	osteoblast specific genes	963:987	osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	963:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	55	theme	type	1003:1006	arg1	receptor					1012:1019	TGF-β type III receptor	997:1019	TGF-β type III receptor	997:1019	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	2	56	theme	critical	238:245	arg1	defects					252:258	48 femur critical size defects	229:258	48 femur critical size defects (3.25 mm in diameter)	229:280	Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery.
24680284	1	57	theme	key	134:136	arg1	regulator					138:146	a key regulator	132:146	a key regulator of bone matrix properties and composition	132:188	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	1	57	theme	key	134:136	arg1	fibroin					114:120	plasma-irradiated silk fibroin	91:120	plasma-irradiated silk fibroin (P-AF)	91:127	We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition.
24680284	2	58	from	mm	266:267	arg1	diameter					272:279	diameter	272:279	diameter	272:279	Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery.
24680284	6	59	theme	TGF-β	997:1001	arg1	genes					983:987	osteoblast specific genes	963:987	osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin)	963:1060	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	6	59	theme	TGF-β	997:1001	arg1	receptor					1012:1019	TGF-β type III receptor	997:1019	TGF-β type III receptor	997:1019	Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin).
24680284	7	60	theme	Runx2	1168:1172	arg1	pathway					1174:1180	Runx2 pathway	1168:1180	Runx2 pathway in P-AF group	1168:1194	These data were the first to show that the properties of bone matrix are regulated, specifically through Runx2 pathway in P-AF group.
24680284	3	61	theme	plasma	609:614	arg1	irradiation					616:626	plasma irradiation	609:626	plasma irradiation	609:626	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	2	62	theme	femur	232:236	arg1	defects					252:258	48 femur critical size defects	229:258	48 femur critical size defects (3.25 mm in diameter)	229:280	Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery.
24680284	3	63	theme	techniques	417:426	arg1	states					407:412	various states	399:412	various states of techniques	399:426	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	3	64	theme	large	476:480	arg1	pores					488:492	the large sized pores	472:492	the large sized pores	472:492	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	3	65	theme	various	399:405	arg1	states					407:412	various states	399:412	various states of techniques	399:426	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	3	66	theme	irradiation	616:626	arg1	addition					597:604	the addition	593:604	the addition of plasma irradiation	593:626	The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation.
24680284	7	67	theme	matrix	1125:1130	arg1	properties					1106:1115	the properties	1102:1115	the properties of bone matrix	1102:1130	These data were the first to show that the properties of bone matrix are regulated, specifically through Runx2 pathway in P-AF group.
24680284	8	68	theme	bone	1368:1371	arg1	volume					1373:1378	trabecular bone volume	1357:1378	trabecular bone volume	1357:1378	Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
24680284	2	69	dep	defects	252:258	arg1	mm					266:267	3.25 mm	261:267	3.25 mm	261:267	Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery.
27163789	8	0	theme	consistent	1636:1645	arg1	association					1647:1657	The consistent association	1632:1657	The consistent association between Calr and Dio2 expression	1632:1690	The consistent association between Calr and Dio2 expression suggests that enhanced expression of these genes facilitate detrimental effects on cartilage integrity.
27163789	3	1	theme	cartilage	737:745	arg1	deposition					747:756	cartilage deposition	737:756	cartilage deposition	737:756	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	3	2	theme	blot	565:568	arg1	analyses					570:577	RT-qPCR and western blot analyses	545:577	analyses	570:577	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	1	3	theme	tissue	274:279	arg1	homeostasis					281:291	prolonged healthy tissue homeostasis	256:291	prolonged healthy tissue homeostasis	256:291	OBJECTIVE To identify intrinsic differences in cartilage gene expression profiles between wild-type- and Dio2-/--mice, as a mechanism to investigate factors that contribute to prolonged healthy tissue homeostasis.
27163789	3	4	from	analyses	570:577	arg1	ATDC5					654:658	ATDC5	654:658	ATDC5	654:658	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	3	4	from	analyses	570:577	arg1	cells					647:651	mouse chondro-progenitor cells	622:651	mouse chondro-progenitor cells (ATDC5)	622:659	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	3	5	theme	western	557:563	arg1	analyses					570:577	RT-qPCR and western blot analyses	545:577	analyses	570:577	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	5	6	theme	proteoglycan	1170:1181	arg1	deposition					1183:1192	decreased proteoglycan deposition	1160:1192	decreased proteoglycan deposition	1160:1192	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	4	7	theme	cutoff	887:892	arg1	threshold					894:902	a cutoff threshold	885:902	|a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||)|	885:934	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	2	8	theme	MouseWG-6	349:357	arg1	microarray-data					323:337	microarray-data	323:337	microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice	323:411	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	2	8	theme	MouseWG-6	349:357	arg1	v2					359:360	Illumina MouseWG-6 v2	340:360	Illumina MouseWG-6 v2	340:360	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	4	9	theme	FC	924:925	arg1	|||1,5|||					929:933	|FC > ||1,5|||	924:933	|FC > ||1,5|||	924:933	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	8	10	theme	cartilage	1775:1783	arg1	integrity					1785:1793	cartilage integrity	1775:1793	cartilage integrity	1775:1793	The consistent association between Calr and Dio2 expression suggests that enhanced expression of these genes facilitate detrimental effects on cartilage integrity.
27163789	4	11	theme	expression	780:789	arg1	analyses					791:798	Differential expression analyses	767:798	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|	759:1070	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	4	12	dep	=	1062:1062	arg1	-1.731					1052:1057	-1.731	1052:1057	-1.731	1052:1057	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	1	13	theme	cartilage	127:135	arg1	profiles					153:160	cartilage gene expression profiles	127:160	cartilage gene expression profiles	127:160	OBJECTIVE To identify intrinsic differences in cartilage gene expression profiles between wild-type- and Dio2-/--mice, as a mechanism to investigate factors that contribute to prolonged healthy tissue homeostasis.
27163789	7	14	theme	cartilage	1609:1617	arg1	destruction					1619:1629	cartilage destruction	1609:1629	cartilage destruction	1609:1629	Functional analyses further showed that upregulation of Calr expression could act as an initiator of cartilage destruction.
27163789	5	15	theme	down	1256:1259	arg1	expression					1266:1275	knocking down Calr expression	1247:1275	knocking down Calr expression	1247:1275	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	1	16	theme	expression	142:151	arg1	profiles					153:160	cartilage gene expression profiles	127:160	cartilage gene expression profiles	127:160	OBJECTIVE To identify intrinsic differences in cartilage gene expression profiles between wild-type- and Dio2-/--mice, as a mechanism to investigate factors that contribute to prolonged healthy tissue homeostasis.
27163789	4	17	theme	located	956:962	arg1	probe					950:954	1 probe	948:954	1 probe located in Calreticulin (Calr)	948:985	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	0	18	theme	Deficient	64:72	arg1	Mice					74:77	Dio2 Deficient Mice	59:77	Dio2 Deficient Mice	59:77	Aberrant Calreticulin Expression in Articular Cartilage of Dio2 Deficient Mice.
27163789	0	19	from	Expression	22:31	arg1	Cartilage					46:54	Articular Cartilage	36:54	Articular Cartilage of Dio2 Deficient Mice	36:77	Aberrant Calreticulin Expression in Articular Cartilage of Dio2 Deficient Mice.
27163789	6	20	dep	CONCLUSION	1342:1351	arg1	demonstrate					1361:1371	demonstrate	1361:1371	demonstrate that the beneficial homeostatic state of articular cartilage in Dio2-/- mice is accompanied with significant lower expression of Calr	1361:1505	CONCLUSION We here demonstrate that the beneficial homeostatic state of articular cartilage in Dio2-/- mice is accompanied with significant lower expression of Calr.
27163789	7	21	theme	Calr	1564:1567	arg1	expression					1569:1578	Calr expression	1564:1578	Calr expression	1564:1578	Functional analyses further showed that upregulation of Calr expression could act as an initiator of cartilage destruction.
27163789	2	22	theme	mechanical	486:495	arg1	conditions					505:514	mechanical loading conditions	486:514	mechanical loading conditions	486:514	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	4	23	theme	cartilage	813:821	arg1	analyses					791:798	Differential expression analyses	767:798	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|	759:1070	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	5	24	theme	matrix	1322:1327	arg1	composition					1329:1339	matrix composition	1322:1339	matrix composition	1322:1339	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	2	25	theme	-mice	407:411	arg1	microarray-data					323:337	microarray-data	323:337	microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice	323:411	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	2	25	theme	-mice	407:411	arg1	v2					359:360	Illumina MouseWG-6 v2	340:360	Illumina MouseWG-6 v2	340:360	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	0	26	theme	Aberrant	0:7	arg1	Expression					22:31	Aberrant Calreticulin Expression	0:31	Aberrant Calreticulin Expression in Articular Cartilage of Dio2 Deficient Mice.	0:78	Aberrant Calreticulin Expression in Articular Cartilage of Dio2 Deficient Mice.
27163789	2	27	theme	independent	471:481	arg1	genes					465:469	differential expressed genes	442:469	differential expressed genes independent of mechanical loading conditions	442:514	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	4	28	theme	Dio2-/-	826:832	arg1	cartilage					813:821	articular cartilage	803:821	|articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr)|	803:985	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	2	29	theme	expressed	455:463	arg1	genes					465:469	differential expressed genes	442:469	differential expressed genes independent of mechanical loading conditions	442:514	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	6	30	theme	articular	1414:1422	arg1	cartilage					1424:1432	articular cartilage	1414:1432	articular cartilage	1414:1432	CONCLUSION We here demonstrate that the beneficial homeostatic state of articular cartilage in Dio2-/- mice is accompanied with significant lower expression of Calr.
27163789	5	31	theme	ATDC5	1139:1143	arg1	cells					1145:1149	ATDC5 cells	1139:1149	ATDC5 cells	1139:1149	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	2	32	theme	forced	519:524	arg1	treadmill-running					526:542	forced treadmill-running	519:542	forced treadmill-running	519:542	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	4	33	theme	=	864:864	arg1	wild-type-mice					846:859	wild-type-mice	846:859	wild-type-mice	846:859	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	4	33	theme	=	864:864	arg1	N					862:862	N = 11	862:867	N = 11	862:867	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	5	34	from	overexpression	1085:1098	arg1	cells					1145:1149	ATDC5 cells	1139:1149	ATDC5 cells	1139:1149	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	0	35	theme	Articular	36:44	arg1	Cartilage					46:54	Articular Cartilage	36:54	Articular Cartilage of Dio2 Deficient Mice	36:77	Aberrant Calreticulin Expression in Articular Cartilage of Dio2 Deficient Mice.
27163789	2	36	theme	conditions	505:514	arg1	independent					471:481	independent	471:481	independent	471:481	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	3	37	theme	direct	688:693	arg1	effect					695:700	the direct effect	684:700	the direct effect of differential Calr expression on cartilage deposition	684:756	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	3	38	theme	RT-qPCR	545:551	arg1	analyses					570:577	RT-qPCR and western blot analyses	545:577	analyses	570:577	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	4	39	dep	threshold	894:902	arg1	FDR					915:917	FDR	915:917	FDR	915:917	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	4	39	dep	threshold	894:902	arg1	<					907:907	P < 0.05	905:912	P < 0.05 (FDR)	905:918	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	4	39	dep	threshold	894:902	arg1	|||1,5|||					929:933	|FC > ||1,5|||	924:933	|FC > ||1,5|||	924:933	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	7	40	theme	Functional	1508:1517	arg1	analyses					1519:1526	Functional analyses	1508:1526	Functional analyses	1508:1526	Functional analyses further showed that upregulation of Calr expression could act as an initiator of cartilage destruction.
27163789	1	41	from	differences	112:122	arg1	profiles					153:160	cartilage gene expression profiles	127:160	cartilage gene expression profiles	127:160	OBJECTIVE To identify intrinsic differences in cartilage gene expression profiles between wild-type- and Dio2-/--mice, as a mechanism to investigate factors that contribute to prolonged healthy tissue homeostasis.
27163789	4	42	from	Calreticulin	967:978	arg1	located					956:962	located	956:962	located	956:962	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	3	43	theme	Calr	718:721	arg1	expression					723:732	differential Calr expression	705:732	differential Calr expression	705:732	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	8	44	from	effects	1764:1770	arg1	integrity					1785:1793	cartilage integrity	1775:1793	cartilage integrity	1775:1793	The consistent association between Calr and Dio2 expression suggests that enhanced expression of these genes facilitate detrimental effects on cartilage integrity.
27163789	3	45	from	overexpression	582:595	arg1	ATDC5					654:658	ATDC5	654:658	ATDC5	654:658	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	3	45	from	overexpression	582:595	arg1	cells					647:651	mouse chondro-progenitor cells	622:651	mouse chondro-progenitor cells (ATDC5)	622:659	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	6	46	theme	significant	1470:1480	arg1	expression					1488:1497	significant lower expression	1470:1497	significant lower expression of Calr	1470:1505	CONCLUSION We here demonstrate that the beneficial homeostatic state of articular cartilage in Dio2-/- mice is accompanied with significant lower expression of Calr.
27163789	4	47	from	located	956:962	arg1	Calr					981:984	Calr	981:984	Calr	981:984	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	4	47	from	located	956:962	arg1	Calreticulin					967:978	Calreticulin	967:978	Calreticulin (Calr)	967:985	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	6	48	theme	homeostatic	1393:1403	arg1	state					1405:1409	the beneficial homeostatic state	1378:1409	the beneficial homeostatic state of articular cartilage in Dio2-/- mice	1378:1448	CONCLUSION We here demonstrate that the beneficial homeostatic state of articular cartilage in Dio2-/- mice is accompanied with significant lower expression of Calr.
27163789	4	49	theme	Dio2-/-	1033:1039	arg1	mice					1041:1044	Dio2-/- mice	1033:1044	Dio2-/- mice (FC = -1.731; P = 0.044)	1033:1069	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	2	50	theme	wild-type	384:392	arg1	-mice					407:411	wild-type and Dio2 -/- -mice	384:411	wild-type and Dio2 -/- -mice	384:411	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	1	51	theme	healthy	266:272	arg1	homeostasis					281:291	prolonged healthy tissue homeostasis	256:291	prolonged healthy tissue homeostasis	256:291	OBJECTIVE To identify intrinsic differences in cartilage gene expression profiles between wild-type- and Dio2-/--mice, as a mechanism to investigate factors that contribute to prolonged healthy tissue homeostasis.
27163789	1	52	theme	prolonged	256:264	arg1	homeostasis					281:291	prolonged healthy tissue homeostasis	256:291	prolonged healthy tissue homeostasis	256:291	OBJECTIVE To identify intrinsic differences in cartilage gene expression profiles between wild-type- and Dio2-/--mice, as a mechanism to investigate factors that contribute to prolonged healthy tissue homeostasis.
27163789	3	53	theme	knockdown	601:609	arg1	analyses					570:577	RT-qPCR and western blot analyses	545:577	analyses	570:577	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	8	54	theme	enhanced	1706:1713	arg1	expression					1715:1724	enhanced expression	1706:1724	enhanced expression of these genes	1706:1739	The consistent association between Calr and Dio2 expression suggests that enhanced expression of these genes facilitate detrimental effects on cartilage integrity.
27163789	5	55	theme	decreased	1160:1168	arg1	deposition					1183:1192	decreased proteoglycan deposition	1160:1192	decreased proteoglycan deposition	1160:1192	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	4	56	theme	P	905:905	arg1	FDR					915:917	FDR	915:917	FDR	915:917	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	4	56	theme	P	905:905	arg1	<					907:907	P < 0.05	905:912	P < 0.05 (FDR)	905:918	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	3	57	from	effect	695:700	arg1	deposition					747:756	cartilage deposition	737:756	cartilage deposition	737:756	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	2	58	theme	cartilage	371:379	arg1	microarray-data					323:337	microarray-data	323:337	microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice	323:411	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	2	58	theme	cartilage	371:379	arg1	v2					359:360	Illumina MouseWG-6 v2	340:360	Illumina MouseWG-6 v2	340:360	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	3	59	theme	overexpression	582:595	arg1	analyses					570:577	RT-qPCR and western blot analyses	545:577	analyses	570:577	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	6	60	theme	beneficial	1382:1391	arg1	state					1405:1409	the beneficial homeostatic state	1378:1409	the beneficial homeostatic state of articular cartilage in Dio2-/- mice	1378:1448	CONCLUSION We here demonstrate that the beneficial homeostatic state of articular cartilage in Dio2-/- mice is accompanied with significant lower expression of Calr.
27163789	4	61	dep	found	996:1000	arg1	downregulated					1016:1028	downregulated	1016:1028	was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044)	992:1069	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	3	62	theme	mouse	622:626	arg1	ATDC5					654:658	ATDC5	654:658	ATDC5	654:658	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	3	62	theme	mouse	622:626	arg1	cells					647:651	mouse chondro-progenitor cells	622:651	mouse chondro-progenitor cells (ATDC5)	622:659	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	8	63	theme	genes	1735:1739	arg1	expression					1715:1724	enhanced expression	1706:1724	enhanced expression of these genes	1706:1739	The consistent association between Calr and Dio2 expression suggests that enhanced expression of these genes facilitate detrimental effects on cartilage integrity.
27163789	3	64	theme	Calr	614:617	arg1	knockdown					601:609	knockdown	601:609	knockdown	601:609	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	3	64	theme	Calr	614:617	arg1	overexpression					582:595	overexpression	582:595	overexpression	582:595	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	8	65	theme	detrimental	1752:1762	arg1	effects					1764:1770	detrimental effects	1752:1770	detrimental effects on cartilage integrity	1752:1793	The consistent association between Calr and Dio2 expression suggests that enhanced expression of these genes facilitate detrimental effects on cartilage integrity.
27163789	3	66	theme	chondro-progenitor	628:645	arg1	ATDC5					654:658	ATDC5	654:658	ATDC5	654:658	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	3	66	theme	chondro-progenitor	628:645	arg1	cells					647:651	mouse chondro-progenitor cells	622:651	mouse chondro-progenitor cells (ATDC5)	622:659	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	6	67	theme	Calr	1502:1505	arg1	expression					1488:1497	significant lower expression	1470:1497	significant lower expression of Calr	1470:1505	CONCLUSION We here demonstrate that the beneficial homeostatic state of articular cartilage in Dio2-/- mice is accompanied with significant lower expression of Calr.
27163789	7	68	theme	destruction	1619:1629	arg1	upregulation					1548:1559	upregulation	1548:1559	upregulation of Calr expression	1548:1578	Functional analyses further showed that upregulation of Calr expression could act as an initiator of cartilage destruction.
27163789	7	68	theme	destruction	1619:1629	arg1	initiator					1596:1604	an initiator	1593:1604	an initiator of cartilage destruction	1593:1629	Functional analyses further showed that upregulation of Calr expression could act as an initiator of cartilage destruction.
27163789	4	69	theme	>	927:927	arg1	|||1,5|||					929:933	|FC > ||1,5|||	924:933	|FC > ||1,5|||	924:933	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	4	70	dep	Dio2-/-	826:832	arg1	resulted					936:943	resulted	936:943	resulted in 1 probe located in Calreticulin (Calr)	936:985	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	4	71	theme	Differential	767:778	arg1	analyses					791:798	Differential expression analyses	767:798	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|	759:1070	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	5	72	theme	knocking	1247:1254	arg1	expression					1266:1275	knocking down Calr expression	1247:1275	knocking down Calr expression	1247:1275	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	1	73	theme	gene	137:140	arg1	profiles					153:160	cartilage gene expression profiles	127:160	cartilage gene expression profiles	127:160	OBJECTIVE To identify intrinsic differences in cartilage gene expression profiles between wild-type- and Dio2-/--mice, as a mechanism to investigate factors that contribute to prolonged healthy tissue homeostasis.
27163789	2	74	theme	Illumina	340:347	arg1	microarray-data					323:337	microarray-data	323:337	microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice	323:411	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	2	74	theme	Illumina	340:347	arg1	v2					359:360	Illumina MouseWG-6 v2	340:360	Illumina MouseWG-6 v2	340:360	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	5	75	theme	Calr	1261:1264	arg1	expression					1266:1275	knocking down Calr expression	1247:1275	knocking down Calr expression	1247:1275	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	0	76	theme	Mice	74:77	arg1	Cartilage					46:54	Articular Cartilage	36:54	Articular Cartilage of Dio2 Deficient Mice	36:77	Aberrant Calreticulin Expression in Articular Cartilage of Dio2 Deficient Mice.
27163789	6	77	from	state	1405:1409	arg1	mice					1445:1448	Dio2-/- mice	1437:1448	Dio2-/- mice	1437:1448	CONCLUSION We here demonstrate that the beneficial homeostatic state of articular cartilage in Dio2-/- mice is accompanied with significant lower expression of Calr.
27163789	5	78	theme	Aggrecan	1218:1225	arg1	expression					1227:1236	lower Aggrecan expression	1212:1236	lower Aggrecan expression	1212:1236	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	7	79	theme	expression	1569:1578	arg1	initiator					1596:1604	an initiator	1593:1604	an initiator of cartilage destruction	1593:1629	Functional analyses further showed that upregulation of Calr expression could act as an initiator of cartilage destruction.
27163789	7	79	theme	expression	1569:1578	arg1	upregulation					1548:1559	upregulation	1548:1559	upregulation of Calr expression	1548:1578	Functional analyses further showed that upregulation of Calr expression could act as an initiator of cartilage destruction.
27163789	3	80	from	knockdown	601:609	arg1	ATDC5					654:658	ATDC5	654:658	ATDC5	654:658	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	3	80	from	knockdown	601:609	arg1	cells					647:651	mouse chondro-progenitor cells	622:651	mouse chondro-progenitor cells (ATDC5)	622:659	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	4	81	dep	mice	1041:1044	arg1	=					1062:1062	=	1062:1062	=	1062:1062	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	4	82	dep	RESULTS	759:765	arg1	analyses					791:798	Differential expression analyses	767:798	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|	759:1070	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	5	83	theme	histological	1294:1305	arg1	differences					1307:1317	histological differences	1294:1317	histological differences of matrix composition	1294:1339	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	4	84	theme	=	837:837	arg1	N					835:835	N = 9	835:839	N = 9	835:839	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	4	84	theme	=	837:837	arg1	Dio2-/-					826:832	Dio2-/-	826:832	Dio2-/-	826:832	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	0	85	theme	Calreticulin	9:20	arg1	Expression					22:31	Aberrant Calreticulin Expression	0:31	Aberrant Calreticulin Expression in Articular Cartilage of Dio2 Deficient Mice.	0:78	Aberrant Calreticulin Expression in Articular Cartilage of Dio2 Deficient Mice.
27163789	4	86	theme	articular	803:811	arg1	cartilage					813:821	articular cartilage	803:821	|articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr)|	803:985	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	3	87	from	cells	647:651	arg1	analyses					570:577	RT-qPCR and western blot analyses	545:577	analyses	570:577	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	2	88	theme	-/-	403:405	arg1	-mice					407:411	wild-type and Dio2 -/- -mice	384:411	wild-type and Dio2 -/- -mice	384:411	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	6	89	theme	cartilage	1424:1432	arg1	state					1405:1409	the beneficial homeostatic state	1378:1409	the beneficial homeostatic state of articular cartilage in Dio2-/- mice	1378:1448	CONCLUSION We here demonstrate that the beneficial homeostatic state of articular cartilage in Dio2-/- mice is accompanied with significant lower expression of Calr.
27163789	1	90	theme	intrinsic	102:110	arg1	mechanism					204:212	a mechanism	202:212	a mechanism to investigate factors that contribute to prolonged healthy tissue homeostasis	202:291	OBJECTIVE To identify intrinsic differences in cartilage gene expression profiles between wild-type- and Dio2-/--mice, as a mechanism to investigate factors that contribute to prolonged healthy tissue homeostasis.
27163789	1	90	theme	intrinsic	102:110	arg1	differences					112:122	intrinsic differences	102:122	intrinsic differences in cartilage gene expression profiles between wild-type- and Dio2-/--mice	102:196	OBJECTIVE To identify intrinsic differences in cartilage gene expression profiles between wild-type- and Dio2-/--mice, as a mechanism to investigate factors that contribute to prolonged healthy tissue homeostasis.
27163789	2	91	theme	differential	442:453	arg1	genes					465:469	differential expressed genes	442:469	differential expressed genes independent of mechanical loading conditions	442:514	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	8	92	theme	Dio2	1676:1679	arg1	expression					1681:1690	Dio2 expression	1676:1690	Dio2 expression	1676:1690	The consistent association between Calr and Dio2 expression suggests that enhanced expression of these genes facilitate detrimental effects on cartilage integrity.
27163789	0	93	theme	Dio2	59:62	arg1	Mice					74:77	Dio2 Deficient Mice	59:77	Dio2 Deficient Mice	59:77	Aberrant Calreticulin Expression in Articular Cartilage of Dio2 Deficient Mice.
27163789	5	94	theme	Calr	1103:1106	arg1	overexpression					1085:1098	overexpression	1085:1098	overexpression of Calr during early chondrogenesis in ATDC5 cells	1085:1149	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	2	95	theme	loading	497:503	arg1	conditions					505:514	mechanical loading conditions	486:514	mechanical loading conditions	486:514	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	3	96	theme	expression	723:732	arg1	effect					695:700	the direct effect	684:700	the direct effect of differential Calr expression on cartilage deposition	684:756	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	2	97	theme	knee	366:369	arg1	cartilage					371:379	knee cartilage	366:379	knee cartilage	366:379	METHODS Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running.
27163789	5	98	theme	early	1115:1119	arg1	chondrogenesis					1121:1134	early chondrogenesis	1115:1134	early chondrogenesis	1115:1134	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	3	99	theme	differential	705:716	arg1	expression					723:732	differential Calr expression	705:732	differential Calr expression	705:732	RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.
27163789	5	100	theme	composition	1329:1339	arg1	differences					1307:1317	histological differences	1294:1317	histological differences of matrix composition	1294:1339	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
27163789	6	101	dep	significant	1470:1480	arg1	lower					1482:1486	lower	1482:1486	lower	1482:1486	CONCLUSION We here demonstrate that the beneficial homeostatic state of articular cartilage in Dio2-/- mice is accompanied with significant lower expression of Calr.
27163789	4	102	theme	wild-type-mice	846:859	arg1	cartilage					813:821	articular cartilage	803:821	|articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr)|	803:985	|RESULTS Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mice (N = 11) while applying a cutoff threshold (P < 0.05 (FDR) and FC > ||1,5||) resulted in 1 probe located in Calreticulin (Calr) that was found significantly downregulated in Dio2-/- mice (FC = -1.731; P = 0.044).|
27163789	6	103	theme	Dio2-/-	1437:1443	arg1	mice					1445:1448	Dio2-/- mice	1437:1448	Dio2-/- mice	1437:1448	CONCLUSION We here demonstrate that the beneficial homeostatic state of articular cartilage in Dio2-/- mice is accompanied with significant lower expression of Calr.
27163789	5	104	theme	lower	1212:1216	arg1	expression					1227:1236	lower Aggrecan expression	1212:1236	lower Aggrecan expression	1212:1236	Furthermore, overexpression of Calr during early chondrogenesis in ATDC5 cells leads to decreased proteoglycan deposition and corresponding lower Aggrecan expression, whereas knocking down Calr expression does not lead to histological differences of matrix composition.
28098142	1	0	theme	ecological	151:160	arg1	endophyte					174:182	an endophyte	171:182	an endophyte	171:182	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	1	0	theme	ecological	151:160	arg1	pathogen					198:205	an insect pathogen	188:205	an insect pathogen	188:205	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	1	0	theme	ecological	151:160	arg1	niche					162:166	a specialized ecological niche	137:166	a specialized ecological niche	137:166	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	5	1	theme	13C	756:758	arg1	amount					746:751	the amount	742:751	the amount of 13C present in root-associated fungal biomass	742:800	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	5	1	theme	13C	756:758	arg1	13C					756:758	13C	756:758	13C present in root-associated fungal biomass	756:800	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	5	2	from	present	760:766	arg1	biomass					794:800	root-associated fungal biomass	771:800	root-associated fungal biomass	771:800	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	5	3	theme	nuclear	896:902	arg1	NMR					924:926	NMR	924:926	NMR	924:926	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	5	3	theme	nuclear	896:902	arg1	resonance					913:921	nuclear magnetic resonance	896:921	nuclear magnetic resonance (NMR) spectroscopy	896:940	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	5	4	theme	magnetic	904:911	arg1	NMR					924:926	NMR	924:926	NMR	924:926	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	5	4	theme	magnetic	904:911	arg1	resonance					913:921	nuclear magnetic resonance	896:921	nuclear magnetic resonance (NMR) spectroscopy	896:940	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	6	5	theme	tripartite	1085:1094	arg1	interaction					1112:1122	a tripartite, and symbiotic, interaction	1083:1122	a tripartite, and symbiotic, interaction	1083:1122	These findings are evidence that the host plant is providing photosynthate to the fungus, likely in exchange for insect-derived nitrogen in a tripartite, and symbiotic, interaction.
28098142	2	6	theme	Metarhizium	289:299	arg1	pathogenicity					272:284	insect pathogenicity	265:284	insect pathogenicity	265:284	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
28098142	2	6	theme	Metarhizium	289:299	arg1	capability					250:259	endophytic capability	239:259	endophytic capability	239:259	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
28098142	2	7	link	insect-derived	344:357	arg1	transfer					368:375	insect-derived nitrogen transfer	344:375	insect-derived nitrogen transfer to a host plant via fungal mycelia	344:410	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
28098142	3	8	link	insect-derived	453:466	arg1	nitrogen					468:475	this insect-derived nitrogen	448:475	this insect-derived nitrogen	448:475	We speculated that in exchange for this insect-derived nitrogen, the plant would provide photosynthate to the fungus.
28098142	2	9	theme	insect-derived	344:357	arg1	transfer					368:375	insect-derived nitrogen transfer	344:375	insect-derived nitrogen transfer to a host plant via fungal mycelia	344:410	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
28098142	4	10	from	carbohydrates	660:672	arg1	complex					719:725	the root/endophyte complex	700:725	the root/endophyte complex	700:725	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	6	11	theme	host	980:983	arg1	plant					985:989	the host plant	976:989	the host plant	976:989	These findings are evidence that the host plant is providing photosynthate to the fungus, likely in exchange for insect-derived nitrogen in a tripartite, and symbiotic, interaction.
28098142	5	12	theme	resonance	913:921	arg1	spectroscopy					929:940	nuclear magnetic resonance (NMR) spectroscopy	896:940	nuclear magnetic resonance (NMR) spectroscopy	896:940	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	3	13	theme	insect-derived	453:466	arg1	nitrogen					468:475	this insect-derived nitrogen	448:475	this insect-derived nitrogen	448:475	We speculated that in exchange for this insect-derived nitrogen, the plant would provide photosynthate to the fungus.
28098142	2	14	theme	insect	265:270	arg1	pathogenicity					272:284	insect pathogenicity	265:284	insect pathogenicity	265:284	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
28098142	6	15	theme	insect-derived	1056:1069	arg1	nitrogen					1071:1078	insect-derived nitrogen	1056:1078	insect-derived nitrogen	1056:1078	These findings are evidence that the host plant is providing photosynthate to the fungus, likely in exchange for insect-derived nitrogen in a tripartite, and symbiotic, interaction.
28098142	5	16	from	biomass	794:800	arg1	present					760:766	present	760:766	present	760:766	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	2	17	theme	host	382:385	arg1	plant					387:391	a host plant	380:391	a host plant	380:391	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
28098142	0	18	from	plant	28:32	arg1	translocation					7:19	Carbon translocation	0:19	Carbon translocation from a plant to an insect-pathogenic endophytic fungus	0:74	Carbon translocation from a plant to an insect-pathogenic endophytic fungus.
28098142	5	19	attach	present	760:766	arg2	13C					756:758	13C	756:758	13C present in root-associated fungal biomass	756:800	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	5	19	attach	present	760:766	arg1	biomass					794:800	root-associated fungal biomass	771:800	root-associated fungal biomass	771:800	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	2	20	dep	capability	250:259	arg1	the					235:237	the	235:237	the	235:237	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
28098142	0	21	theme	Carbon	0:5	arg1	translocation					7:19	Carbon translocation	0:19	Carbon translocation from a plant to an insect-pathogenic endophytic fungus	0:74	Carbon translocation from a plant to an insect-pathogenic endophytic fungus.
28098142	6	22	from	exchange	1043:1050	arg1	interaction					1112:1122	a tripartite, and symbiotic, interaction	1083:1122	a tripartite, and symbiotic, interaction	1083:1122	These findings are evidence that the host plant is providing photosynthate to the fungus, likely in exchange for insect-derived nitrogen in a tripartite, and symbiotic, interaction.
28098142	4	23	theme	fungal-specific	644:658	arg1	chitin					689:694	chitin	689:694	chitin	689:694	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	4	23	theme	fungal-specific	644:658	arg1	trehalose					675:683	trehalose	675:683	trehalose	675:683	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	4	23	theme	fungal-specific	644:658	arg1	carbohydrates					660:672	fungal-specific carbohydrates	644:672	fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex	644:725	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	5	24	theme	root-associated	771:785	arg1	biomass					794:800	root-associated fungal biomass	771:800	root-associated fungal biomass	771:800	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	4	25	theme	subsequent	607:616	arg1	translocation					618:630	the subsequent translocation	603:630	the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex	603:725	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	5	26	theme	21-day	809:814	arg1	period					816:821	a 21-day period	807:821	a 21-day period	807:821	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	1	27	theme	common	104:109	arg1	fungus					116:121	a common soil fungus	102:121	a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen	102:205	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	1	27	theme	common	104:109	arg1	robertsii					89:97	Metarhizium robertsii	77:97	Metarhizium robertsii	77:97	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	1	28	theme	insect	191:196	arg1	pathogen					198:205	an insect pathogen	188:205	an insect pathogen	188:205	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	1	28	theme	insect	191:196	arg1	niche					162:166	a specialized ecological niche	137:166	a specialized ecological niche	137:166	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	5	29	theme	present	760:766	arg1	13C					756:758	13C	756:758	13C present in root-associated fungal biomass	756:800	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	2	30	theme	fungal	397:402	arg1	mycelia					404:410	fungal mycelia	397:410	fungal mycelia	397:410	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
28098142	5	31	theme	fungal	837:842	arg1	carbohydrates					844:856	fungal carbohydrates	837:856	fungal carbohydrates	837:856	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	0	32	theme	insect-pathogenic	40:56	arg1	fungus					69:74	an insect-pathogenic endophytic fungus	37:74	an insect-pathogenic endophytic fungus	37:74	Carbon translocation from a plant to an insect-pathogenic endophytic fungus.
28098142	1	33	theme	soil	111:114	arg1	fungus					116:121	a common soil fungus	102:121	a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen	102:205	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	1	33	theme	soil	111:114	arg1	robertsii					89:97	Metarhizium robertsii	77:97	Metarhizium robertsii	77:97	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	5	34	theme	fungal	787:792	arg1	biomass					794:800	root-associated fungal biomass	771:800	root-associated fungal biomass	771:800	We determined the amount of 13C present in root-associated fungal biomass over a 21-day period by extracting fungal carbohydrates and analysing their composition using nuclear magnetic resonance (NMR) spectroscopy.
28098142	2	35	theme	active	327:332	arg1	method					334:339	an active method	324:339	an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia	324:410	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
28098142	4	36	dep	carbohydrates	660:672	arg1	chitin					689:694	chitin	689:694	chitin	689:694	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	4	36	dep	carbohydrates	660:672	arg1	trehalose					675:683	trehalose	675:683	trehalose	675:683	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	4	36	dep	carbohydrates	660:672	arg1	carbohydrates					660:672	fungal-specific carbohydrates	644:672	fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex	644:725	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	4	37	theme	root/endophyte	704:717	arg1	complex					719:725	the root/endophyte complex	700:725	the root/endophyte complex	700:725	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	6	38	theme	symbiotic	1101:1109	arg1	interaction					1112:1122	a tripartite, and symbiotic, interaction	1083:1122	a tripartite, and symbiotic, interaction	1083:1122	These findings are evidence that the host plant is providing photosynthate to the fungus, likely in exchange for insect-derived nitrogen in a tripartite, and symbiotic, interaction.
28098142	2	39	theme	endophytic	239:248	arg1	capability					250:259	endophytic capability	239:259	endophytic capability	239:259	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
28098142	1	40	theme	specialized	139:149	arg1	endophyte					174:182	an endophyte	171:182	an endophyte	171:182	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	1	40	theme	specialized	139:149	arg1	pathogen					198:205	an insect pathogen	188:205	an insect pathogen	188:205	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	1	40	theme	specialized	139:149	arg1	niche					162:166	a specialized ecological niche	137:166	a specialized ecological niche	137:166	Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen.
28098142	4	41	theme	13C	576:578	arg1	incorporation					559:571	the incorporation	555:571	the incorporation of 13C into photosynthate	555:597	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	4	41	theme	13C	576:578	arg1	translocation					618:630	the subsequent translocation	603:630	the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex	603:725	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	2	42	theme	transfer	368:375	arg1	method					334:339	an active method	324:339	an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia	324:410	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
28098142	4	43	theme	13C	635:637	arg1	incorporation					559:571	the incorporation	555:571	the incorporation of 13C into photosynthate	555:597	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	4	43	theme	13C	635:637	arg1	translocation					618:630	the subsequent translocation	603:630	the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex	603:725	By using 13CO2, we show the incorporation of 13C into photosynthate and the subsequent translocation of 13C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex.
28098142	0	44	theme	endophytic	58:67	arg1	fungus					69:74	an insect-pathogenic endophytic fungus	37:74	an insect-pathogenic endophytic fungus	37:74	Carbon translocation from a plant to an insect-pathogenic endophytic fungus.
28098142	6	45	link	insect-derived	1056:1069	arg1	nitrogen					1071:1078	insect-derived nitrogen	1056:1078	insect-derived nitrogen	1056:1078	These findings are evidence that the host plant is providing photosynthate to the fungus, likely in exchange for insect-derived nitrogen in a tripartite, and symbiotic, interaction.
28098142	2	46	theme	nitrogen	359:366	arg1	transfer					368:375	insect-derived nitrogen transfer	344:375	insect-derived nitrogen transfer to a host plant via fungal mycelia	344:410	Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia.
26003303	0	0	theme	antimicrobial	79:91	arg1	films					93:97	chitosan/cellulose micro crystals (CMC) antimicrobial films	39:97	chitosan/cellulose micro crystals (CMC) antimicrobial films	39:97	Investigation of curcumin release from chitosan/cellulose micro crystals (CMC) antimicrobial films.
26003303	1	1	theme	X-ray	311:315	arg1	diffraction					317:327	X-ray diffraction	311:327	X-ray diffraction (XRD)	311:333	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	1	theme	X-ray	311:315	arg1	XRD					330:332	XRD	330:332	XRD	330:332	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	2	theme	composite	234:242	arg1	films					244:248	chitosan/cellulose micro crystals composite films	200:248	chitosan/cellulose micro crystals composite films	200:248	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	2	3	theme	different	458:466	arg1	RH					487:488	RH	487:488	RH	487:488	The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully.
26003303	2	3	theme	different	458:466	arg1	humidity					477:484	different relative humidity	458:484	different relative humidity (RH) environments	458:502	The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully.
26003303	3	4	theme	physiological	653:665	arg1	fluid					667:671	the physiological fluid	649:671	the physiological fluid (PF) at 37 °C	649:685	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	3	4	theme	physiological	653:665	arg1	PF					674:675	PF	674:675	PF	674:675	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	2	5	theme	isotherm	553:560	arg1	model					562:566	the GAB isotherm model	545:566	the GAB isotherm model	545:566	The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully.
26003303	2	6	theme	GAB	549:551	arg1	model					562:566	the GAB isotherm model	545:566	the GAB isotherm model	545:566	The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully.
26003303	3	7	from	°C	684:685	arg1	fluid					667:671	the physiological fluid	649:671	the physiological fluid (PF) at 37 °C	649:685	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	3	7	from	°C	684:685	arg1	behavior					637:644	their curcumin release behavior	614:644	their curcumin release behavior in the physiological fluid (PF) at 37 °C	614:685	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	3	7	from	°C	684:685	arg1	PF					674:675	PF	674:675	PF	674:675	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	1	8	theme	scanning	339:346	arg1	SEM					369:371	SEM	369:371	SEM	369:371	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	8	theme	scanning	339:346	arg1	microscopy					357:366	scanning electron microscopy	339:366	scanning electron microscopy (SEM)	339:372	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	4	9	theme	antimicrobial	787:799	arg1	action					801:806	fair antimicrobial action	782:806	fair antimicrobial action against bacteria and fungi	782:833	Finally, the films showed fair antimicrobial action against bacteria and fungi.
26003303	2	10	theme	humidity	477:484	arg1	environments					491:502	different relative humidity (RH) environments	458:502	different relative humidity (RH) environments	458:502	The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully.
26003303	3	11	from	behavior	637:644	arg1	fluid					667:671	the physiological fluid	649:671	the physiological fluid (PF) at 37 °C	649:685	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	3	11	from	behavior	637:644	arg1	°C					684:685	37 °C	681:685	37 °C	681:685	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	3	11	from	behavior	637:644	arg1	PF					674:675	PF	674:675	PF	674:675	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	4	12	theme	fair	782:785	arg1	action					801:806	fair antimicrobial action	782:806	fair antimicrobial action against bacteria and fungi	782:833	Finally, the films showed fair antimicrobial action against bacteria and fungi.
26003303	2	13	theme	absorption	400:409	arg1	behavior					411:418	The equilibrium moisture absorption behavior	375:418	The equilibrium moisture absorption behavior of these films	375:433	The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully.
26003303	3	14	theme	kinetic	695:701	arg1	data					703:706	the kinetic data	691:706	the kinetic data obtained	691:715	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	2	15	theme	equilibrium	379:389	arg1	behavior					411:418	The equilibrium moisture absorption behavior	375:418	The equilibrium moisture absorption behavior of these films	375:433	The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully.
26003303	0	16	theme	curcumin	17:24	arg1	Investigation					0:12	Investigation	0:12	Investigation of curcumin	0:24	Investigation of curcumin release from chitosan/cellulose micro crystals (CMC) antimicrobial films.
26003303	1	17	theme	thermo	276:281	arg1	TGA					305:307	TGA	305:307	TGA	305:307	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	17	theme	thermo	276:281	arg1	analysis					295:302	thermo gravimetric analysis	276:302	thermo gravimetric analysis (TGA)	276:308	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	18	theme	electron	348:355	arg1	SEM					369:371	SEM	369:371	SEM	369:371	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	18	theme	electron	348:355	arg1	microscopy					357:366	scanning electron microscopy	339:366	scanning electron microscopy (SEM)	339:372	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	0	19	theme	chitosan/cellulose	39:56	arg1	films					93:97	chitosan/cellulose micro crystals (CMC) antimicrobial films	39:97	chitosan/cellulose micro crystals (CMC) antimicrobial films	39:97	Investigation of curcumin release from chitosan/cellulose micro crystals (CMC) antimicrobial films.
26003303	1	20	theme	gravimetric	283:293	arg1	TGA					305:307	TGA	305:307	TGA	305:307	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	20	theme	gravimetric	283:293	arg1	analysis					295:302	thermo gravimetric analysis	276:302	thermo gravimetric analysis (TGA)	276:308	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	0	21	theme	crystals	64:71	arg1	films					93:97	chitosan/cellulose micro crystals (CMC) antimicrobial films	39:97	chitosan/cellulose micro crystals (CMC) antimicrobial films	39:97	Investigation of curcumin release from chitosan/cellulose micro crystals (CMC) antimicrobial films.
26003303	2	22	theme	relative	468:475	arg1	RH					487:488	RH	487:488	RH	487:488	The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully.
26003303	2	22	theme	relative	468:475	arg1	humidity					477:484	different relative humidity	458:484	different relative humidity (RH) environments	458:502	The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully.
26003303	1	23	theme	novel	114:118	arg1	method					159:164	the novel 'vapor induced phase inversion' (VIPI) method	110:164	the novel 'vapor induced phase inversion' (VIPI) method	110:164	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	0	24	theme	micro	58:62	arg1	films					93:97	chitosan/cellulose micro crystals (CMC) antimicrobial films	39:97	chitosan/cellulose micro crystals (CMC) antimicrobial films	39:97	Investigation of curcumin release from chitosan/cellulose micro crystals (CMC) antimicrobial films.
26003303	1	25	theme	vapor	121:125	arg1	method					159:164	the novel 'vapor induced phase inversion' (VIPI) method	110:164	the novel 'vapor induced phase inversion' (VIPI) method	110:164	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	3	26	theme	Higuchi	741:747	arg1	model					749:753	Higuchi model	741:753	Higuchi model	741:753	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	3	27	theme	curcumin	620:627	arg1	behavior					637:644	their curcumin release behavior	614:644	their curcumin release behavior in the physiological fluid (PF) at 37 °C	614:685	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	1	28	dep	curcumin	184:191	arg1	loaded					193:198	loaded	193:198	loaded chitosan/cellulose micro crystals composite films	193:248	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	28	dep	curcumin	184:191	arg1	characterized					254:266	characterized	254:266	characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM)	254:372	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	29	theme	induced	127:133	arg1	method					159:164	the novel 'vapor induced phase inversion' (VIPI) method	110:164	the novel 'vapor induced phase inversion' (VIPI) method	110:164	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	30	theme	chitosan/cellulose	200:217	arg1	films					244:248	chitosan/cellulose micro crystals composite films	200:248	chitosan/cellulose micro crystals composite films	200:248	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	0	31	theme	CMC	74:76	arg1	films					93:97	chitosan/cellulose micro crystals (CMC) antimicrobial films	39:97	chitosan/cellulose micro crystals (CMC) antimicrobial films	39:97	Investigation of curcumin release from chitosan/cellulose micro crystals (CMC) antimicrobial films.
26003303	3	32	theme	release	629:635	arg1	behavior					637:644	their curcumin release behavior	614:644	their curcumin release behavior in the physiological fluid (PF) at 37 °C	614:685	The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model.
26003303	1	33	theme	phase	135:139	arg1	inversion					141:149	phase inversion'	135:150	the novel 'vapor induced phase inversion' (VIPI) method	110:164	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	33	theme	phase	135:139	arg1	VIPI					153:156	VIPI	153:156	VIPI	153:156	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	34	theme	micro	219:223	arg1	films					244:248	chitosan/cellulose micro crystals composite films	200:248	chitosan/cellulose micro crystals composite films	200:248	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	2	35	theme	films	429:433	arg1	behavior					411:418	The equilibrium moisture absorption behavior	375:418	The equilibrium moisture absorption behavior of these films	375:433	The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully.
26003303	2	36	theme	moisture	391:398	arg1	behavior					411:418	The equilibrium moisture absorption behavior	375:418	The equilibrium moisture absorption behavior of these films	375:433	The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully.
26003303	1	37	theme	inversion	141:149	arg1	method					159:164	the novel 'vapor induced phase inversion' (VIPI) method	110:164	the novel 'vapor induced phase inversion' (VIPI) method	110:164	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
26003303	1	38	theme	crystals	225:232	arg1	films					244:248	chitosan/cellulose micro crystals composite films	200:248	chitosan/cellulose micro crystals composite films	200:248	Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
24376168	0	0	theme	cellulose	80:88	arg1	solvation					67:75	solvation	67:75	solvation of cellulose and enzymatic hydrolysis using response surface methodology	67:148	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.
24376168	2	1	theme	time	483:486	arg1	effects					463:469	the effects	459:469	the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields	459:622	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	5	2	theme	objective	1063:1071	arg1	functions					1073:1081	the selected objective functions	1050:1081	the selected objective functions	1050:1081	The significance of the correlations and adequacy of these models were statistically tested for the selected objective functions.
24376168	3	3	theme	°C	711:712	arg1	conditions					694:703	The optimum pretreatment conditions	669:703	The optimum pretreatment conditions of 50 °C and 40 min	669:723	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	1	4	theme	organic	239:245	arg1	approach					303:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	2	5	theme	reaction	474:481	arg1	time					483:486	reaction time	474:486	reaction time (20-60 min)	474:498	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	2	5	theme	reaction	474:481	arg1	min					495:497	20-60 min	489:497	20-60 min	489:497	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	1	6	theme	solvent-based	247:259	arg1	approach					303:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	0	7	theme	enzymatic	94:102	arg1	hydrolysis					104:113	enzymatic hydrolysis	94:113	enzymatic hydrolysis	94:113	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.
24376168	3	8	theme	optimum	673:679	arg1	conditions					694:703	The optimum pretreatment conditions	669:703	The optimum pretreatment conditions of 50 °C and 40 min	669:723	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	2	9	theme	sugar	562:566	arg1	yield					568:572	total reducing sugar yield	547:572	total reducing sugar yield	547:572	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	7	10	theme	large	1251:1255	arg1	biorefineries					1263:1275	large scale biorefineries	1251:1275	large scale biorefineries using sugars released from SSB for producing various biofuels	1251:1337	Results from this study can be applied to large scale biorefineries using sugars released from SSB for producing various biofuels.
24376168	1	11	theme	lignocellulose	261:274	arg1	approach					303:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	0	12	from	bagasse	49:55	arg1	Optimization					0:11	Optimization	0:11	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.	0:149	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.
24376168	2	13	theme	reducing	553:560	arg1	yield					568:572	total reducing sugar yield	547:572	total reducing sugar yield	547:572	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	2	14	theme	glucose	609:615	arg1	yields					617:622	overall glucose yields	601:622	overall glucose yields	601:622	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	1	15	theme	fractionation	276:288	arg1	approach					303:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	2	16	theme	overall	601:607	arg1	yields					617:622	overall glucose yields	601:622	overall glucose yields	601:622	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	2	17	theme	composite	389:397	arg1	design					399:404	RSM's central composite design	375:404	RSM's central composite design	375:404	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	0	18	theme	hydrolysis	104:113	arg1	solvation					67:75	solvation	67:75	solvation of cellulose and enzymatic hydrolysis using response surface methodology	67:148	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.
24376168	5	19	theme	selected	1054:1061	arg1	functions					1073:1081	the selected objective functions	1050:1081	the selected objective functions	1050:1081	The significance of the correlations and adequacy of these models were statistically tested for the selected objective functions.
24376168	3	20	theme	glucose	767:773	arg1	yield					775:779	86% overall glucose yield	755:779	86% overall glucose yield	755:779	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	1	21	theme	sweet	186:190	arg1	SSB					209:211	SSB	209:211	SSB	209:211	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	1	21	theme	sweet	186:190	arg1	bagasse					200:206	sweet sorghum bagasse	186:206	sweet sorghum bagasse (SSB)	186:212	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	2	22	from	effects	463:469	arg1	yield					568:572	total reducing sugar yield	547:572	total reducing sugar yield	547:572	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	2	22	from	effects	463:469	arg1	delignification					530:544	delignification	530:544	delignification	530:544	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	2	22	from	effects	463:469	arg1	yields					617:622	overall glucose yields	601:622	overall glucose yields	601:622	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	2	22	from	effects	463:469	arg1	digestibility					582:594	glucan digestibility	575:594	glucan digestibility	575:594	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	1	23	theme	sorghum	192:198	arg1	SSB					209:211	SSB	209:211	SSB	209:211	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	1	23	theme	sorghum	192:198	arg1	bagasse					200:206	sweet sorghum bagasse	186:206	sweet sorghum bagasse (SSB)	186:212	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	1	24	theme	pretreatment	290:301	arg1	approach					303:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	2	25	theme	glucan	575:580	arg1	digestibility					582:594	glucan digestibility	575:594	glucan digestibility	575:594	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	2	26	theme	pretreatment-hydrolysis	636:658	arg1	process					660:666	a pretreatment-hydrolysis process	634:666	a pretreatment-hydrolysis process	634:666	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	2	27	theme	central	381:387	arg1	design					399:404	RSM's central composite design	375:404	RSM's central composite design	375:404	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	0	28	theme	release	22:28	arg1	Optimization					0:11	Optimization	0:11	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.	0:149	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.
24376168	7	29	from	study	1227:1231	arg1	Results					1209:1215	Results	1209:1215	Results from this study	1209:1231	Results from this study can be applied to large scale biorefineries using sugars released from SSB for producing various biofuels.
24376168	0	30	theme	surface	130:136	arg1	methodology					138:148	response surface methodology	121:148	response surface methodology	121:148	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.
24376168	7	31	theme	various	1322:1328	arg1	biofuels					1330:1337	various biofuels	1322:1337	various biofuels	1322:1337	Results from this study can be applied to large scale biorefineries using sugars released from SSB for producing various biofuels.
24376168	2	32	theme	temperature	504:514	arg1	effects					463:469	the effects	459:469	the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields	459:622	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	0	33	theme	sugar	16:20	arg1	release					22:28	sugar release	16:28	sugar release	16:28	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.
24376168	0	34	theme	response	121:128	arg1	methodology					138:148	response surface methodology	121:148	response surface methodology	121:148	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.
24376168	5	35	theme	models	1013:1018	arg1	adequacy					995:1002	adequacy	995:1002	adequacy of these models	995:1018	The significance of the correlations and adequacy of these models were statistically tested for the selected objective functions.
24376168	5	35	theme	models	1013:1018	arg1	significance					958:969	The significance	954:969	The significance of the correlations	954:989	The significance of the correlations and adequacy of these models were statistically tested for the selected objective functions.
24376168	6	36	theme	pretreatment	1096:1107	arg1	min					1163:1165	49.1 °C and 39.2 min	1146:1165	49.1 °C and 39.2 min which matched the experimental data well	1146:1206	The optimum pretreatment condition predicted by the model was 49.1 °C and 39.2 min which matched the experimental data well.
24376168	6	36	theme	pretreatment	1096:1107	arg1	condition					1109:1117	The optimum pretreatment condition	1084:1117	The optimum pretreatment condition predicted by the model	1084:1140	The optimum pretreatment condition predicted by the model was 49.1 °C and 39.2 min which matched the experimental data well.
24376168	2	37	theme	experimental	415:426	arg1	matrix					428:433	a batch experimental matrix	407:433	a batch experimental matrix	407:433	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	0	38	theme	sweet	35:39	arg1	bagasse					49:55	sweet sorghum bagasse	35:55	sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology	35:148	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.
24376168	3	39	theme	min	721:723	arg1	conditions					694:703	The optimum pretreatment conditions	669:703	The optimum pretreatment conditions of 50 °C and 40 min	669:723	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	6	40	theme	experimental	1185:1196	arg1	data					1198:1201	the experimental data	1181:1201	the experimental data	1181:1201	The optimum pretreatment condition predicted by the model was 49.1 °C and 39.2 min which matched the experimental data well.
24376168	2	41	theme	batch	409:413	arg1	matrix					428:433	a batch experimental matrix	407:433	a batch experimental matrix	407:433	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	4	42	theme	dependent	923:931	arg1	variables					933:941	independent and dependent variables	907:941	independent and dependent variables using RSM	907:951	An effort has also been made to obtain predictive models to illustrate the correlation between independent and dependent variables using RSM.
24376168	3	43	theme	61	786:787	arg1	%					788:788	%	788:788	%	788:788	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	3	44	theme	51.4	732:735	arg1	%					736:736	%	736:736	%	736:736	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	3	45	theme	86	755:756	arg1	%					757:757	%	757:757	%	757:757	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	1	46	theme	response	330:337	arg1	RSM					360:362	RSM	360:362	RSM	360:362	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	1	46	theme	response	330:337	arg1	methodology					347:357	response surface methodology	330:357	response surface methodology (RSM)	330:363	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	2	47	theme	total	547:551	arg1	yield					568:572	total reducing sugar yield	547:572	total reducing sugar yield	547:572	Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process.
24376168	7	48	theme	scale	1257:1261	arg1	biorefineries					1263:1275	large scale biorefineries	1251:1275	large scale biorefineries using sugars released from SSB for producing various biofuels	1251:1337	Results from this study can be applied to large scale biorefineries using sugars released from SSB for producing various biofuels.
24376168	6	49	theme	optimum	1088:1094	arg1	min					1163:1165	49.1 °C and 39.2 min	1146:1165	49.1 °C and 39.2 min which matched the experimental data well	1146:1206	The optimum pretreatment condition predicted by the model was 49.1 °C and 39.2 min which matched the experimental data well.
24376168	6	49	theme	optimum	1088:1094	arg1	condition					1109:1117	The optimum pretreatment condition	1084:1117	The optimum pretreatment condition predicted by the model	1084:1140	The optimum pretreatment condition predicted by the model was 49.1 °C and 39.2 min which matched the experimental data well.
24376168	0	50	theme	sorghum	41:47	arg1	bagasse					49:55	sweet sorghum bagasse	35:55	sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology	35:148	Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.
24376168	1	51	theme	surface	339:345	arg1	RSM					360:362	RSM	360:362	RSM	360:362	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	1	51	theme	surface	339:345	arg1	methodology					347:357	response surface methodology	330:357	response surface methodology (RSM)	330:363	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	4	52	theme	predictive	851:860	arg1	models					862:867	predictive models	851:867	predictive models	851:867	An effort has also been made to obtain predictive models to illustrate the correlation between independent and dependent variables using RSM.
24376168	5	53	theme	correlations	978:989	arg1	adequacy					995:1002	adequacy	995:1002	adequacy of these models	995:1018	The significance of the correlations and adequacy of these models were statistically tested for the selected objective functions.
24376168	5	53	theme	correlations	978:989	arg1	significance					958:969	The significance	954:969	The significance of the correlations	954:989	The significance of the correlations and adequacy of these models were statistically tested for the selected objective functions.
24376168	3	54	theme	xylose	798:803	arg1	yield					805:809	61% overall xylose yield	786:809	61% overall xylose yield	786:809	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	3	55	theme	%	736:736	arg1	delignification					738:752	51.4% delignification	732:752	51.4% delignification	732:752	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	3	56	theme	overall	759:765	arg1	yield					775:779	86% overall glucose yield	755:779	86% overall glucose yield	755:779	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	3	57	theme	%	788:788	arg1	yield					805:809	61% overall xylose yield	786:809	61% overall xylose yield	786:809	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	1	58	theme	cellulose	217:225	arg1	approach					303:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	3	59	theme	overall	790:796	arg1	yield					805:809	61% overall xylose yield	786:809	61% overall xylose yield	786:809	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	1	60	theme	solvent	227:233	arg1	approach					303:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach	215:310	To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM).
24376168	4	61	theme	independent	907:917	arg1	variables					933:941	independent and dependent variables	907:941	independent and dependent variables using RSM	907:951	An effort has also been made to obtain predictive models to illustrate the correlation between independent and dependent variables using RSM.
24376168	3	62	theme	pretreatment	681:692	arg1	conditions					694:703	The optimum pretreatment conditions	669:703	The optimum pretreatment conditions of 50 °C and 40 min	669:723	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
24376168	7	63	attach	released	1290:1297	arg2	sugars					1283:1288	sugars	1283:1288	sugars released from SSB for producing various biofuels	1283:1337	Results from this study can be applied to large scale biorefineries using sugars released from SSB for producing various biofuels.
24376168	7	63	attach	released	1290:1297	arg1	SSB					1304:1306	SSB	1304:1306	SSB for producing various biofuels	1304:1337	Results from this study can be applied to large scale biorefineries using sugars released from SSB for producing various biofuels.
24376168	3	64	theme	%	757:757	arg1	yield					775:779	86% overall glucose yield	755:779	86% overall glucose yield	755:779	The optimum pretreatment conditions of 50 °C and 40 min led to 51.4% delignification, 86% overall glucose yield, and 61% overall xylose yield.
29115567	6	0	theme	mechanical	997:1006	arg1	properties					1008:1017	satisfactory mechanical properties	984:1017	satisfactory mechanical properties	984:1017	The decellularized ECM exhibited satisfactory mechanical properties.
29115567	4	1	theme	significant	748:758	arg1	disruption					760:769	significant disruption	748:769	significant disruption of the morphology or structure of the ECM	748:811	Cellular components, including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM.
29115567	9	2	theme	ECM	1539:1541	arg1	cues					1518:1521	biological and chemical cues	1494:1521	biological and chemical cues of native human ECM	1494:1541	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	3	3	theme	successive	509:518	arg1	treatments					542:551	successive physical and chemical treatments	509:551	successive physical and chemical treatments	509:551	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	9	4	theme	ideal	1554:1558	arg1	material					1569:1576	an ideal scaffold material	1551:1576	an ideal scaffold material for autologous and allograft tissue engineering	1551:1624	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	9	4	theme	ideal	1554:1558	arg1	ECM					1478:1480	the decellularized ECM	1459:1480	the decellularized ECM	1459:1480	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	1	5	theme	tissues	242:248	arg1	regeneration					218:229	the regeneration	214:229	the regeneration of adipose tissues	214:248	Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues.
29115567	6	6	theme	satisfactory	984:995	arg1	properties					1008:1017	satisfactory mechanical properties	984:1017	satisfactory mechanical properties	984:1017	The decellularized ECM exhibited satisfactory mechanical properties.
29115567	7	7	theme	adipose‑derived	1061:1075	arg1	cells					1082:1086	human adipose‑derived stem cells	1055:1086	human adipose‑derived stem cells	1055:1086	Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors.
29115567	2	8	theme	structural	342:351	arg1	components					368:377	the structural and functional components	338:377	the structural and functional components of the resultant scaffold material	338:412	The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material.
29115567	8	9	theme	collagen	1390:1397	arg1	type					1399:1402	collagen type I	1390:1404	collagen type I scaffolds	1390:1414	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	8	10	theme	induced	1319:1325	arg1	cells					1327:1331	induced cells	1319:1331	induced cells	1319:1331	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	9	11	contain	containing	1483:1492	arg2	cues					1518:1521	biological and chemical cues	1494:1521	biological and chemical cues of native human ECM	1494:1541	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	9	11	contain	containing	1483:1492	arg1	material					1569:1576	an ideal scaffold material	1551:1576	an ideal scaffold material for autologous and allograft tissue engineering	1551:1624	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	9	11	contain	containing	1483:1492	arg1	ECM					1478:1480	the decellularized ECM	1459:1480	the decellularized ECM	1459:1480	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	7	12	theme	stem	1077:1080	arg1	cells					1082:1086	human adipose‑derived stem cells	1055:1086	human adipose‑derived stem cells	1055:1086	Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors.
29115567	2	13	theme	adipose	279:285	arg1	scaffolds					298:306	adipose tissue ECM scaffolds	279:306	adipose tissue ECM scaffolds	279:306	The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material.
29115567	4	14	theme	nucleic	701:707	arg1	acids					709:713	nucleic acids	701:713	nucleic acids	701:713	Cellular components, including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM.
29115567	7	15	link	adipose‑derived	1061:1075	arg1	cells					1082:1086	human adipose‑derived stem cells	1055:1086	human adipose‑derived stem cells	1055:1086	Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors.
29115567	0	16	theme	physical	91:98	arg1	treatments					113:122	physical and chemical treatments	91:122	physical and chemical treatments	91:122	Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments.
29115567	8	17	from	cells	1381:1385	arg1	scaffolds					1406:1414	collagen type I scaffolds	1390:1414	collagen type I scaffolds	1390:1414	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	4	18	theme	ECM	809:811	arg1	structure					792:800	structure	792:800	structure	792:800	Cellular components, including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM.
29115567	4	18	theme	ECM	809:811	arg1	morphology					778:787	morphology	778:787	morphology	778:787	Cellular components, including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM.
29115567	3	19	theme	repeated	564:571	arg1	cycles					585:590	repeated freeze‑thaw cycles	564:590	repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion	564:667	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	9	20	theme	allograft	1597:1605	arg1	engineering					1614:1624	autologous and allograft tissue engineering	1582:1624	autologous and allograft tissue engineering	1582:1624	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	1	21	theme	extracellular	148:160	arg1	ECM					170:172	ECM	170:172	ECM	170:172	Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues.
29115567	1	21	theme	extracellular	148:160	arg1	matrix					162:167	Decellularized adipose extracellular matrix	125:167	Decellularized adipose extracellular matrix (ECM)	125:173	Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues.
29115567	0	22	theme	treatments	113:122	arg1	combination					76:86	a combination	74:86	a combination of physical and chemical treatments	74:122	Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments.
29115567	6	23	theme	decellularized	955:968	arg1	ECM					970:972	The decellularized ECM	951:972	The decellularized ECM	951:972	The decellularized ECM exhibited satisfactory mechanical properties.
29115567	5	24	theme	soluble	893:899	arg1	collagen					901:908	acid/pepsin soluble collagen	881:908	acid/pepsin soluble collagen	881:908	The compositions of major ECM components were evaluated, including acid/pepsin soluble collagen, sulfated glycosaminoglycan and laminin.
29115567	9	25	theme	scaffold	1560:1567	arg1	material					1569:1576	an ideal scaffold material	1551:1576	an ideal scaffold material for autologous and allograft tissue engineering	1551:1624	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	9	25	theme	scaffold	1560:1567	arg1	ECM					1478:1480	the decellularized ECM	1459:1480	the decellularized ECM	1459:1480	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	9	26	theme	autologous	1582:1591	arg1	engineering					1614:1624	autologous and allograft tissue engineering	1582:1624	autologous and allograft tissue engineering	1582:1624	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	2	27	theme	scaffold	396:403	arg1	material					405:412	the resultant scaffold material	382:412	the resultant scaffold material	382:412	The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material.
29115567	0	28	theme	chemical	104:111	arg1	treatments					113:122	physical and chemical treatments	91:122	physical and chemical treatments	91:122	Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments.
29115567	9	29	theme	decellularized	1463:1476	arg1	material					1569:1576	an ideal scaffold material	1551:1576	an ideal scaffold material for autologous and allograft tissue engineering	1551:1624	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	9	29	theme	decellularized	1463:1476	arg1	ECM					1478:1480	the decellularized ECM	1459:1480	the decellularized ECM	1459:1480	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	7	30	theme	seeding	1025:1031	arg1	experiments					1033:1043	Cell seeding experiments	1020:1043	Cell seeding experiments involving human adipose‑derived stem cells	1020:1086	Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors.
29115567	8	31	located	observed	1304:1311	arg1	scaffolds					1344:1352	the ECM scaffolds	1336:1352	the ECM scaffolds	1336:1352	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	8	31	located	observed	1304:1311	arg2	levels					1245:1250	Higher levels	1238:1250	Higher levels of glycerol‑3‑phosphate dehydrogenase activity	1238:1297	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	2	32	theme	resultant	386:394	arg1	material					405:412	the resultant scaffold material	382:412	the resultant scaffold material	382:412	The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material.
29115567	8	33	theme	ECM	1340:1342	arg1	scaffolds					1344:1352	the ECM scaffolds	1336:1352	the ECM scaffolds	1336:1352	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	3	34	theme	chemical	533:540	arg1	treatments					542:551	successive physical and chemical treatments	509:551	successive physical and chemical treatments	509:551	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	7	35	theme	exogenous	1203:1211	arg1	factors					1229:1235	exogenous differentiation factors	1203:1235	exogenous differentiation factors	1203:1235	Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors.
29115567	3	36	from	tissues	487:493	arg1	scaffold					459:466	an acellular ECM scaffold	442:466	an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion	442:667	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	4	37	theme	structure	792:800	arg1	disruption					760:769	significant disruption	748:769	significant disruption of the morphology or structure of the ECM	748:811	Cellular components, including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM.
29115567	9	38	theme	tissue	1607:1612	arg1	engineering					1614:1624	autologous and allograft tissue engineering	1582:1624	autologous and allograft tissue engineering	1582:1624	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	7	39	theme	differentiation	1213:1227	arg1	factors					1229:1235	exogenous differentiation factors	1203:1235	exogenous differentiation factors	1203:1235	Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors.
29115567	5	40	theme	sulfated	911:918	arg1	glycosaminoglycan					920:936	sulfated glycosaminoglycan	911:936	sulfated glycosaminoglycan	911:936	The compositions of major ECM components were evaluated, including acid/pepsin soluble collagen, sulfated glycosaminoglycan and laminin.
29115567	8	41	theme	type	1399:1402	arg1	scaffolds					1406:1414	collagen type I scaffolds	1390:1414	collagen type I scaffolds	1390:1414	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	8	42	theme	induced	1373:1379	arg1	cells					1381:1385	induced cells	1373:1385	induced cells in collagen type I scaffolds	1373:1414	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	7	43	theme	Cell	1020:1023	arg1	experiments					1033:1043	Cell seeding experiments	1020:1043	Cell seeding experiments involving human adipose‑derived stem cells	1020:1086	Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors.
29115567	2	44	theme	ECM	294:296	arg1	scaffolds					298:306	adipose tissue ECM scaffolds	279:306	adipose tissue ECM scaffolds	279:306	The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material.
29115567	4	45	theme	Cellular	670:677	arg1	components					679:688	Cellular components	670:688	Cellular components	670:688	Cellular components, including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM.
29115567	4	45	theme	Cellular	670:677	arg1	acids					709:713	nucleic acids	701:713	nucleic acids	701:713	Cellular components, including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM.
29115567	9	46	theme	biological	1494:1503	arg1	cues					1518:1521	biological and chemical cues	1494:1521	biological and chemical cues of native human ECM	1494:1541	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	7	47	theme	decellularized	1107:1120	arg1	ECM					1122:1124	the decellularized ECM	1103:1124	the decellularized ECM	1103:1124	Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors.
29115567	2	48	theme	tissue	287:292	arg1	scaffolds					298:306	adipose tissue ECM scaffolds	279:306	adipose tissue ECM scaffolds	279:306	The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material.
29115567	7	49	theme	human	1055:1059	arg1	cells					1082:1086	human adipose‑derived stem cells	1055:1086	human adipose‑derived stem cells	1055:1086	Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors.
29115567	3	50	theme	human	473:477	arg1	cycles					585:590	repeated freeze‑thaw cycles	564:590	repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion	564:667	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	3	50	theme	human	473:477	arg1	tissues					487:493	human adipose tissues	473:493	human adipose tissues	473:493	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	0	51	theme	adipose	46:52	arg1	matrix					61:66	acellular adipose tissue matrix	36:66	acellular adipose tissue matrix	36:66	Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments.
29115567	3	52	theme	polar	620:624	arg1	extraction					634:643	polar solvent extraction	620:643	polar solvent extraction	620:643	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	3	53	theme	ECM	455:457	arg1	scaffold					459:466	an acellular ECM scaffold	442:466	an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion	442:667	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	0	54	theme	acellular	36:44	arg1	matrix					61:66	acellular adipose tissue matrix	36:66	acellular adipose tissue matrix	36:66	Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments.
29115567	9	55	theme	chemical	1509:1516	arg1	cues					1518:1521	biological and chemical cues	1494:1521	biological and chemical cues of native human ECM	1494:1541	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	3	56	theme	current	419:425	arg1	study					427:431	The current study	415:431	The current study	415:431	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	3	57	theme	solvent	626:632	arg1	extraction					634:643	polar solvent extraction	620:643	polar solvent extraction	620:643	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	2	58	theme	material	405:412	arg1	components					368:377	the structural and functional components	338:377	the structural and functional components of the resultant scaffold material	338:412	The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material.
29115567	0	59	theme	matrix	61:66	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments.
29115567	0	59	theme	matrix	61:66	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments.
29115567	5	60	theme	components	844:853	arg1	compositions					818:829	The compositions	814:829	The compositions of major ECM components	814:853	The compositions of major ECM components were evaluated, including acid/pepsin soluble collagen, sulfated glycosaminoglycan and laminin.
29115567	8	61	theme	dehydrogenase	1276:1288	arg1	activity					1290:1297	glycerol‑3‑phosphate dehydrogenase activity	1255:1297	glycerol‑3‑phosphate dehydrogenase activity	1255:1297	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	8	62	theme	glycerol‑3‑phosphate	1255:1274	arg1	activity					1290:1297	glycerol‑3‑phosphate dehydrogenase activity	1255:1297	glycerol‑3‑phosphate dehydrogenase activity	1255:1297	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	0	63	theme	tissue	54:59	arg1	matrix					61:66	acellular adipose tissue matrix	36:66	acellular adipose tissue matrix	36:66	Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments.
29115567	8	64	theme	Higher	1238:1243	arg1	levels					1245:1250	Higher levels	1238:1250	Higher levels of glycerol‑3‑phosphate dehydrogenase activity	1238:1297	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	3	65	theme	physical	520:527	arg1	treatments					542:551	successive physical and chemical treatments	509:551	successive physical and chemical treatments	509:551	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	1	66	theme	Decellularized	125:138	arg1	ECM					170:172	ECM	170:172	ECM	170:172	Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues.
29115567	1	66	theme	Decellularized	125:138	arg1	matrix					162:167	Decellularized adipose extracellular matrix	125:167	Decellularized adipose extracellular matrix (ECM)	125:173	Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues.
29115567	4	67	theme	morphology	778:787	arg1	disruption					760:769	significant disruption	748:769	significant disruption of the morphology or structure of the ECM	748:811	Cellular components, including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM.
29115567	9	68	theme	native	1526:1531	arg1	ECM					1539:1541	native human ECM	1526:1541	native human ECM	1526:1541	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	7	69	theme	inductive	1138:1146	arg1	microenvironment					1148:1163	an inductive microenvironment	1135:1163	an inductive microenvironment for adipogenesis	1135:1180	Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors.
29115567	3	70	theme	enzymatic	649:657	arg1	digestion					659:667	enzymatic digestion	649:667	enzymatic digestion	649:667	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	4	71	dep	morphology	778:787	arg1	the					774:776	the	774:776	the	774:776	Cellular components, including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM.
29115567	1	72	theme	adipose	140:146	arg1	ECM					170:172	ECM	170:172	ECM	170:172	Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues.
29115567	1	72	theme	adipose	140:146	arg1	matrix					162:167	Decellularized adipose extracellular matrix	125:167	Decellularized adipose extracellular matrix (ECM)	125:173	Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues.
29115567	3	73	theme	freeze‑thaw	573:583	arg1	cycles					585:590	repeated freeze‑thaw cycles	564:590	repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion	564:667	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	9	74	theme	human	1533:1537	arg1	ECM					1539:1541	native human ECM	1526:1541	native human ECM	1526:1541	In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.
29115567	1	75	used	used	184:187	arg2	ECM					170:172	ECM	170:172	ECM	170:172	Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues.
29115567	1	75	used	used	184:187	arg2	matrix					162:167	Decellularized adipose extracellular matrix	125:167	Decellularized adipose extracellular matrix (ECM)	125:173	Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues.
29115567	2	76	theme	distinct	316:323	arg1	effects					325:331	distinct effects	316:331	distinct effects	316:331	The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material.
29115567	3	77	theme	adipose	479:485	arg1	cycles					585:590	repeated freeze‑thaw cycles	564:590	repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion	564:667	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	3	77	theme	adipose	479:485	arg1	tissues					487:493	human adipose tissues	473:493	human adipose tissues	473:493	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	8	78	theme	activity	1290:1297	arg1	levels					1245:1250	Higher levels	1238:1250	Higher levels of glycerol‑3‑phosphate dehydrogenase activity	1238:1297	Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds.
29115567	5	79	theme	acid/pepsin	881:891	arg1	collagen					901:908	acid/pepsin soluble collagen	881:908	acid/pepsin soluble collagen	881:908	The compositions of major ECM components were evaluated, including acid/pepsin soluble collagen, sulfated glycosaminoglycan and laminin.
29115567	3	80	theme	acellular	445:453	arg1	scaffold					459:466	an acellular ECM scaffold	442:466	an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion	442:667	The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion.
29115567	5	81	theme	ECM	840:842	arg1	components					844:853	major ECM components	834:853	major ECM components	834:853	The compositions of major ECM components were evaluated, including acid/pepsin soluble collagen, sulfated glycosaminoglycan and laminin.
29115567	5	82	theme	major	834:838	arg1	components					844:853	major ECM components	834:853	major ECM components	834:853	The compositions of major ECM components were evaluated, including acid/pepsin soluble collagen, sulfated glycosaminoglycan and laminin.
29115567	2	83	theme	functional	357:366	arg1	components					368:377	the structural and functional components	338:377	the structural and functional components of the resultant scaffold material	338:412	The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material.
29115567	1	84	theme	adipose	234:240	arg1	tissues					242:248	adipose tissues	234:248	adipose tissues	234:248	Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues.
28081233	3	0	from	effects	411:417	arg1	interactions					472:483	host-pathogen interactions	458:483	host-pathogen interactions	458:483	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	9	1	theme	NF-κB-mediated	1666:1679	arg1	pathways					1681:1688	p38MAPK- and NF-κB-mediated pathways	1653:1688	p38MAPK- and NF-κB-mediated pathways	1653:1688	Capsular composition may play a role in the differential inflammatory response induced by the ST258-KP strains since capsular polysaccharides purified from bacterial cells affect NLRP3 and pro-IL-1β gene expression through p38MAPK- and NF-κB-mediated pathways.
28081233	3	2	theme	clades	525:530	arg1	representatives					497:511	representatives	497:511	representatives of ST258-KP clades, KKBO-1 and KK207-1,	497:551	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	5	3	theme	IL-1β	920:924	arg1	production					926:935	IL-1β production	920:935	IL-1β production by monocytes and dendritic cells	920:968	Significant differences between the strains were found in their ability to induce the production of IL-1β: KK207-1/clade I was much less effective than KKBO-1/clade II in inducing IL-1β production by monocytes and dendritic cells.
28081233	0	4	theme	ST258	94:98	arg1	pneumoniae					111:120	the Pandemic ST258 Klebsiella pneumoniae	81:120	the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases	81:169	Differences in Inflammatory Response Induced by Two Representatives of Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases.
28081233	0	5	from	Differences	0:10	arg1	Response					28:35	Inflammatory Response	15:35	Inflammatory Response	15:35	Differences in Inflammatory Response Induced by Two Representatives of Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases.
28081233	9	6	theme	pro-IL-1β	1619:1627	arg1	expression					1634:1643	pro-IL-1β gene expression	1619:1643	pro-IL-1β gene expression	1619:1643	Capsular composition may play a role in the differential inflammatory response induced by the ST258-KP strains since capsular polysaccharides purified from bacterial cells affect NLRP3 and pro-IL-1β gene expression through p38MAPK- and NF-κB-mediated pathways.
28081233	6	7	theme	live	1019:1022	arg1	cells					1024:1028	live cells	1019:1028	live cells	1019:1028	The activation of NLRP3 inflammasome pathway by live cells and/or purified capsular polysaccharides was studied in monocytes and dendritic cells.
28081233	0	8	theme	Pandemic	85:92	arg1	pneumoniae					111:120	the Pandemic ST258 Klebsiella pneumoniae	81:120	the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases	81:169	Differences in Inflammatory Response Induced by Two Representatives of Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases.
28081233	7	9	theme	NLRP3	1148:1152	arg1	glibenclamide					1131:1143	glibenclamide	1131:1143	glibenclamide	1131:1143	We found that glibenclamide, a NLRP3 inhibitor, inhibits more than 90% of the production of mature IL-1β induced by KKBO1 and KK207-1.
28081233	7	9	theme	NLRP3	1148:1152	arg1	inhibitor					1154:1162	a NLRP3 inhibitor	1146:1162	a NLRP3 inhibitor	1146:1162	We found that glibenclamide, a NLRP3 inhibitor, inhibits more than 90% of the production of mature IL-1β induced by KKBO1 and KK207-1.
28081233	4	10	theme	inflammatory	716:727	arg1	cytokines					729:737	inflammatory cytokines	716:737	inflammatory cytokines	716:737	The two ST258-KP strains strongly induced the production of inflammatory cytokines.
28081233	5	11	theme	dendritic	954:962	arg1	cells					964:968	dendritic cells	954:968	dendritic cells	954:968	Significant differences between the strains were found in their ability to induce the production of IL-1β: KK207-1/clade I was much less effective than KKBO-1/clade II in inducing IL-1β production by monocytes and dendritic cells.
28081233	0	12	theme	pneumoniae	111:120	arg1	Lineage					129:135	the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage	81:135	the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases	81:169	Differences in Inflammatory Response Induced by Two Representatives of Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases.
28081233	8	13	theme	protein	1350:1356	arg1	expression					1358:1367	pro-IL-1β gene and protein expression	1331:1367	expression	1358:1367	KK207-1 was always less efficient compared to KKBO-1 in: a) inducing NLRP3 and pro-IL-1β gene and protein expression; b) in inducing caspase-1 activation and pro-IL-1β cleavage.
28081233	3	14	theme	phenotype	445:453	arg1	effects					411:417	the effects	407:417	the effects of the different capsular phenotype on host-pathogen interactions	407:483	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	0	15	theme	Klebsiella	100:109	arg1	pneumoniae					111:120	the Pandemic ST258 Klebsiella pneumoniae	81:120	the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases	81:169	Differences in Inflammatory Response Induced by Two Representatives of Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases.
28081233	11	16	theme	immune	1963:1968	arg1	recognition					1970:1980	innate immune recognition	1956:1980	innate immune recognition	1956:1980	On the whole, our data suggest that the change in capsular phenotype may help bacterial cells of clade I to partially escape innate immune recognition and IL-1β-mediated inflammation.
28081233	7	17	theme	production	1195:1204	arg1	%					1186:1186	more than 90%	1174:1186	more than 90% of the production of mature IL-1β induced by KKBO1 and KK207-1	1174:1249	We found that glibenclamide, a NLRP3 inhibitor, inhibits more than 90% of the production of mature IL-1β induced by KKBO1 and KK207-1.
28081233	7	17	theme	production	1195:1204	arg1	production					1195:1204	the production	1191:1204	the production of mature IL-1β induced by KKBO1 and KK207-1	1191:1249	We found that glibenclamide, a NLRP3 inhibitor, inhibits more than 90% of the production of mature IL-1β induced by KKBO1 and KK207-1.
28081233	4	18	theme	ST258-KP	664:671	arg1	strains					673:679	The two ST258-KP strains	656:679	The two ST258-KP strains	656:679	The two ST258-KP strains strongly induced the production of inflammatory cytokines.
28081233	9	19	theme	ST258-KP	1524:1531	arg1	strains					1533:1539	the ST258-KP strains	1520:1539	the ST258-KP strains	1520:1539	Capsular composition may play a role in the differential inflammatory response induced by the ST258-KP strains since capsular polysaccharides purified from bacterial cells affect NLRP3 and pro-IL-1β gene expression through p38MAPK- and NF-κB-mediated pathways.
28081233	6	20	theme	pathway	1008:1014	arg1	activation					975:984	The activation	971:984	The activation of NLRP3 inflammasome pathway by live cells and/or purified capsular polysaccharides	971:1069	The activation of NLRP3 inflammasome pathway by live cells and/or purified capsular polysaccharides was studied in monocytes and dendritic cells.
28081233	2	21	theme	Kb	330:331	arg1	region					341:346	a 215 Kb genomic region	324:346	a 215 Kb genomic region that includes the cluster of capsule genes	324:389	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	3	22	theme	different	426:434	arg1	phenotype					445:453	the different capsular phenotype	422:453	the different capsular phenotype	422:453	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	5	23	theme	IL-1β	840:844	arg1	production					826:835	the production	822:835	the production of IL-1β	822:844	Significant differences between the strains were found in their ability to induce the production of IL-1β: KK207-1/clade I was much less effective than KKBO-1/clade II in inducing IL-1β production by monocytes and dendritic cells.
28081233	5	24	theme	Significant	740:750	arg1	differences					752:762	Significant differences	740:762	Significant differences between the strains	740:782	Significant differences between the strains were found in their ability to induce the production of IL-1β: KK207-1/clade I was much less effective than KKBO-1/clade II in inducing IL-1β production by monocytes and dendritic cells.
28081233	9	25	theme	bacterial	1586:1594	arg1	cells					1596:1600	bacterial cells	1586:1600	bacterial cells	1586:1600	Capsular composition may play a role in the differential inflammatory response induced by the ST258-KP strains since capsular polysaccharides purified from bacterial cells affect NLRP3 and pro-IL-1β gene expression through p38MAPK- and NF-κB-mediated pathways.
28081233	3	26	theme	capsular	436:443	arg1	phenotype					445:453	the different capsular phenotype	422:453	the different capsular phenotype	422:453	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	6	27	theme	inflammasome	995:1006	arg1	pathway					1008:1014	NLRP3 inflammasome pathway	989:1014	NLRP3 inflammasome pathway	989:1014	The activation of NLRP3 inflammasome pathway by live cells and/or purified capsular polysaccharides was studied in monocytes and dendritic cells.
28081233	0	28	theme	Lineage	129:135	arg1	Clades					71:76	Clades	71:76	Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases	71:169	Differences in Inflammatory Response Induced by Two Representatives of Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases.
28081233	3	29	theme	myeloid	597:603	arg1	cells					615:619	myeloid dendritic cells	597:619	myeloid dendritic cells	597:619	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	9	30	theme	Capsular	1430:1437	arg1	composition					1439:1449	Capsular composition	1430:1449	Capsular composition	1430:1449	Capsular composition may play a role in the differential inflammatory response induced by the ST258-KP strains since capsular polysaccharides purified from bacterial cells affect NLRP3 and pro-IL-1β gene expression through p38MAPK- and NF-κB-mediated pathways.
28081233	0	31	theme	Inflammatory	15:26	arg1	Response					28:35	Inflammatory Response	15:35	Inflammatory Response	15:35	Differences in Inflammatory Response Induced by Two Representatives of Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases.
28081233	6	32	theme	NLRP3	989:993	arg1	pathway					1008:1014	NLRP3 inflammasome pathway	989:1014	NLRP3 inflammasome pathway	989:1014	The activation of NLRP3 inflammasome pathway by live cells and/or purified capsular polysaccharides was studied in monocytes and dendritic cells.
28081233	0	33	theme	Clonal	122:127	arg1	Lineage					129:135	the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage	81:135	the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases	81:169	Differences in Inflammatory Response Induced by Two Representatives of Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases.
28081233	8	34	dep	efficient	1276:1284	arg1	b					1370:1370	b	1370:1370	b	1370:1370	KK207-1 was always less efficient compared to KKBO-1 in: a) inducing NLRP3 and pro-IL-1β gene and protein expression; b) in inducing caspase-1 activation and pro-IL-1β cleavage.
28081233	8	34	dep	efficient	1276:1284	arg1	a					1309:1309	a	1309:1309	a	1309:1309	KK207-1 was always less efficient compared to KKBO-1 in: a) inducing NLRP3 and pro-IL-1β gene and protein expression; b) in inducing caspase-1 activation and pro-IL-1β cleavage.
28081233	8	34	dep	efficient	1276:1284	arg1	compared					1286:1293	compared	1286:1293	compared to KKBO-1 in	1286:1306	KK207-1 was always less efficient compared to KKBO-1 in: a) inducing NLRP3 and pro-IL-1β gene and protein expression; b) in inducing caspase-1 activation and pro-IL-1β cleavage.
28081233	8	34	dep	efficient	1276:1284	arg1	inducing					1312:1319	inducing	1312:1319	inducing NLRP3 and pro-IL-1β gene and protein expression	1312:1367	KK207-1 was always less efficient compared to KKBO-1 in: a) inducing NLRP3 and pro-IL-1β gene and protein expression; b) in inducing caspase-1 activation and pro-IL-1β cleavage.
28081233	11	35	theme	innate	1956:1961	arg1	recognition					1970:1980	innate immune recognition	1956:1980	innate immune recognition	1956:1980	On the whole, our data suggest that the change in capsular phenotype may help bacterial cells of clade I to partially escape innate immune recognition and IL-1β-mediated inflammation.
28081233	7	36	theme	IL-1β	1216:1220	arg1	production					1195:1204	the production	1191:1204	the production of mature IL-1β induced by KKBO1 and KK207-1	1191:1249	We found that glibenclamide, a NLRP3 inhibitor, inhibits more than 90% of the production of mature IL-1β induced by KKBO1 and KK207-1.
28081233	4	37	theme	cytokines	729:737	arg1	production					702:711	the production	698:711	the production of inflammatory cytokines	698:737	The two ST258-KP strains strongly induced the production of inflammatory cytokines.
28081233	0	38	theme	KPC-Type	147:154	arg1	Carbapenemases					156:169	KPC-Type Carbapenemases	147:169	KPC-Type Carbapenemases	147:169	Differences in Inflammatory Response Induced by Two Representatives of Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases.
28081233	2	39	theme	ST258-KP	193:200	arg1	pathogens					270:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	2	39	theme	ST258-KP	193:200	arg1	strains					203:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	3	40	theme	dendritic	605:613	arg1	cells					615:619	myeloid dendritic cells	597:619	myeloid dendritic cells	597:619	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	2	41	theme	genes	385:389	arg1	cluster					366:372	the cluster	362:372	the cluster of capsule genes	362:389	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	3	42	theme	host-pathogen	458:470	arg1	interactions					472:483	host-pathogen interactions	458:483	host-pathogen interactions	458:483	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	8	43	theme	caspase-1	1385:1393	arg1	activation					1395:1404	caspase-1 activation	1385:1404	caspase-1 activation	1385:1404	KK207-1 was always less efficient compared to KKBO-1 in: a) inducing NLRP3 and pro-IL-1β gene and protein expression; b) in inducing caspase-1 activation and pro-IL-1β cleavage.
28081233	2	44	theme	hospital-acquired	252:268	arg1	pathogens					270:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	2	44	theme	hospital-acquired	252:268	arg1	strains					203:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	11	45	from	change	1871:1876	arg1	phenotype					1890:1898	capsular phenotype	1881:1898	capsular phenotype	1881:1898	On the whole, our data suggest that the change in capsular phenotype may help bacterial cells of clade I to partially escape innate immune recognition and IL-1β-mediated inflammation.
28081233	7	46	theme	mature	1209:1214	arg1	IL-1β					1216:1220	mature IL-1β	1209:1220	mature IL-1β	1209:1220	We found that glibenclamide, a NLRP3 inhibitor, inhibits more than 90% of the production of mature IL-1β induced by KKBO1 and KK207-1.
28081233	3	47	from	hosts	649:653	arg1	cells					615:619	myeloid dendritic cells	597:619	myeloid dendritic cells	597:619	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	3	47	from	hosts	649:653	arg1	monocytes					583:591	monocytes	583:591	monocytes	583:591	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	2	48	theme	pneumoniae	181:190	arg1	pathogens					270:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	2	48	theme	pneumoniae	181:190	arg1	strains					203:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	2	49	theme	multidrug-resistant	232:250	arg1	pathogens					270:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	2	49	theme	multidrug-resistant	232:250	arg1	strains					203:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	5	50	located	found	789:793	arg2	differences					752:762	Significant differences	740:762	Significant differences between the strains	740:782	Significant differences between the strains were found in their ability to induce the production of IL-1β: KK207-1/clade I was much less effective than KKBO-1/clade II in inducing IL-1β production by monocytes and dendritic cells.
28081233	5	50	located	found	789:793	arg1	ability					804:810	their ability to induce the production of IL-1β	798:844	their ability to induce the production of IL-1β	798:844	Significant differences between the strains were found in their ability to induce the production of IL-1β: KK207-1/clade I was much less effective than KKBO-1/clade II in inducing IL-1β production by monocytes and dendritic cells.
28081233	9	51	theme	differential	1474:1485	arg1	response					1500:1507	the differential inflammatory response	1470:1507	the differential inflammatory response induced by the ST258-KP strains	1470:1539	Capsular composition may play a role in the differential inflammatory response induced by the ST258-KP strains since capsular polysaccharides purified from bacterial cells affect NLRP3 and pro-IL-1β gene expression through p38MAPK- and NF-κB-mediated pathways.
28081233	11	52	theme	capsular	1881:1888	arg1	phenotype					1890:1898	capsular phenotype	1881:1898	capsular phenotype	1881:1898	On the whole, our data suggest that the change in capsular phenotype may help bacterial cells of clade I to partially escape innate immune recognition and IL-1β-mediated inflammation.
28081233	6	53	theme	dendritic	1100:1108	arg1	cells					1110:1114	dendritic cells	1100:1114	dendritic cells	1100:1114	The activation of NLRP3 inflammasome pathway by live cells and/or purified capsular polysaccharides was studied in monocytes and dendritic cells.
28081233	2	54	theme	most	216:219	arg1	pathogens					270:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	2	54	theme	most	216:219	arg1	strains					203:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	2	55	theme	widespread	221:230	arg1	pathogens					270:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	the most widespread multidrug-resistant hospital-acquired pathogens	212:278	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	2	55	theme	widespread	221:230	arg1	strains					203:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains	181:209	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	3	56	theme	ST258-KP	516:523	arg1	KK207-1					544:550	KK207-1	544:550	KK207-1	544:550	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	3	56	theme	ST258-KP	516:523	arg1	KKBO-1					533:538	KKBO-1	533:538	KKBO-1	533:538	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	3	56	theme	ST258-KP	516:523	arg1	clades					525:530	ST258-KP clades	516:530	ST258-KP clades	516:530	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	10	57	from	KK207-1	1749:1755	arg1	polysaccharides					1728:1742	capsular polysaccharides	1719:1742	capsular polysaccharides from KK207-1	1719:1755	In each of these functions, capsular polysaccharides from KK207-1 were significantly less efficient compared to those purified from KKBO-1.
28081233	8	58	theme	pro-IL-1β	1410:1418	arg1	cleavage					1420:1427	pro-IL-1β cleavage	1410:1427	pro-IL-1β cleavage	1410:1427	KK207-1 was always less efficient compared to KKBO-1 in: a) inducing NLRP3 and pro-IL-1β gene and protein expression; b) in inducing caspase-1 activation and pro-IL-1β cleavage.
28081233	11	59	theme	IL-1β-mediated	1986:1999	arg1	inflammation					2001:2012	IL-1β-mediated inflammation	1986:2012	IL-1β-mediated inflammation	1986:2012	On the whole, our data suggest that the change in capsular phenotype may help bacterial cells of clade I to partially escape innate immune recognition and IL-1β-mediated inflammation.
28081233	10	60	theme	capsular	1719:1726	arg1	polysaccharides					1728:1742	capsular polysaccharides	1719:1742	capsular polysaccharides from KK207-1	1719:1755	In each of these functions, capsular polysaccharides from KK207-1 were significantly less efficient compared to those purified from KKBO-1.
28081233	10	61	from	efficient	1781:1789	arg1	each					1694:1697	each	1694:1697	each	1694:1697	In each of these functions, capsular polysaccharides from KK207-1 were significantly less efficient compared to those purified from KKBO-1.
28081233	10	61	from	efficient	1781:1789	arg1	functions					1708:1716	these functions	1702:1716	these functions	1702:1716	In each of these functions, capsular polysaccharides from KK207-1 were significantly less efficient compared to those purified from KKBO-1.
28081233	11	62	theme	bacterial	1909:1917	arg1	cells					1919:1923	bacterial cells	1909:1923	bacterial cells of clade I	1909:1934	On the whole, our data suggest that the change in capsular phenotype may help bacterial cells of clade I to partially escape innate immune recognition and IL-1β-mediated inflammation.
28081233	3	63	theme	competent	639:647	arg1	hosts					649:653	human immune competent hosts	626:653	human immune competent hosts	626:653	To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts.
28081233	2	64	theme	genomic	333:339	arg1	region					341:346	a 215 Kb genomic region	324:346	a 215 Kb genomic region that includes the cluster of capsule genes	324:389	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	8	65	theme	pro-IL-1β	1331:1339	arg1	gene					1341:1344	pro-IL-1β gene and protein expression	1331:1367	gene	1341:1344	KK207-1 was always less efficient compared to KKBO-1 in: a) inducing NLRP3 and pro-IL-1β gene and protein expression; b) in inducing caspase-1 activation and pro-IL-1β cleavage.
28081233	0	66	theme	Clades	71:76	arg1	Representatives					52:66	Two Representatives	48:66	Two Representatives of Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases	48:169	Differences in Inflammatory Response Induced by Two Representatives of Clades of the Pandemic ST258 Klebsiella pneumoniae Clonal Lineage Producing KPC-Type Carbapenemases.
28081233	9	67	theme	p38MAPK-	1653:1660	arg1	pathways					1681:1688	p38MAPK- and NF-κB-mediated pathways	1653:1688	p38MAPK- and NF-κB-mediated pathways	1653:1688	Capsular composition may play a role in the differential inflammatory response induced by the ST258-KP strains since capsular polysaccharides purified from bacterial cells affect NLRP3 and pro-IL-1β gene expression through p38MAPK- and NF-κB-mediated pathways.
28081233	10	68	from	each	1694:1697	arg1	efficient					1781:1789	efficient	1781:1789	efficient	1781:1789	In each of these functions, capsular polysaccharides from KK207-1 were significantly less efficient compared to those purified from KKBO-1.
28081233	2	69	theme	capsule	377:383	arg1	genes					385:389	capsule genes	377:389	capsule genes	377:389	pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes.
28081233	11	70	theme	clade	1928:1932	arg1	cells					1919:1923	bacterial cells	1909:1923	bacterial cells of clade I	1909:1934	On the whole, our data suggest that the change in capsular phenotype may help bacterial cells of clade I to partially escape innate immune recognition and IL-1β-mediated inflammation.
28081233	6	71	theme	capsular	1046:1053	arg1	polysaccharides					1055:1069	purified capsular polysaccharides	1037:1069	purified capsular polysaccharides	1037:1069	The activation of NLRP3 inflammasome pathway by live cells and/or purified capsular polysaccharides was studied in monocytes and dendritic cells.
28081233	9	72	theme	inflammatory	1487:1498	arg1	response					1500:1507	the differential inflammatory response	1470:1507	the differential inflammatory response induced by the ST258-KP strains	1470:1539	Capsular composition may play a role in the differential inflammatory response induced by the ST258-KP strains since capsular polysaccharides purified from bacterial cells affect NLRP3 and pro-IL-1β gene expression through p38MAPK- and NF-κB-mediated pathways.
28081233	9	73	theme	capsular	1547:1554	arg1	polysaccharides					1556:1570	capsular polysaccharides	1547:1570	capsular polysaccharides purified from bacterial cells	1547:1600	Capsular composition may play a role in the differential inflammatory response induced by the ST258-KP strains since capsular polysaccharides purified from bacterial cells affect NLRP3 and pro-IL-1β gene expression through p38MAPK- and NF-κB-mediated pathways.
28081233	9	74	theme	gene	1629:1632	arg1	expression					1634:1643	pro-IL-1β gene expression	1619:1643	pro-IL-1β gene expression	1619:1643	Capsular composition may play a role in the differential inflammatory response induced by the ST258-KP strains since capsular polysaccharides purified from bacterial cells affect NLRP3 and pro-IL-1β gene expression through p38MAPK- and NF-κB-mediated pathways.
28081233	6	75	theme	purified	1037:1044	arg1	polysaccharides					1055:1069	purified capsular polysaccharides	1037:1069	purified capsular polysaccharides	1037:1069	The activation of NLRP3 inflammasome pathway by live cells and/or purified capsular polysaccharides was studied in monocytes and dendritic cells.
26467229	8	0	theme	fragmentation	1538:1550	arg1	patterns					1552:1559	fragmentation patterns	1538:1559	fragmentation patterns	1538:1559	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	8	1	theme	mass	1618:1621	arg1	spectrometry					1623:1634	second-order mass spectrometry	1605:1634	second-order mass spectrometry	1605:1634	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	6	2	from	cleavage	1244:1251	arg1	RESULTS					1141:1147	RESULTS The main fragment ions	1141:1170	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1141:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	2	from	cleavage	1244:1251	arg1	spectra					1185:1191	the MS/MS spectra	1175:1191	the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1175:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	7	3	theme	cross-ring	1377:1386	arg1	cleavage					1388:1395	cross-ring cleavage	1377:1395	cross-ring cleavage	1377:1395	Surprisingly, cross-ring cleavage and dehydration of the precursor ions were rarely observed in both lithiated and sodiated sucrose.
26467229	11	4	theme	hydrogen	2032:2039	arg1	migration					2041:2049	The hydrogen migration	2028:2049	The hydrogen migration of the hydroxyl group at C3 of the fructose unit	2028:2098	The hydrogen migration of the hydroxyl group at C3 of the fructose unit caused the glycosidic bond decomposition of lithiated sucrose.
26467229	6	5	theme	MS/MS	1179:1183	arg1	spectra					1185:1191	the MS/MS spectra	1175:1191	the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1175:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	9	6	theme	sodium	1825:1830	arg1	adducts					1832:1838	sodium adducts	1825:1838	sodium adducts	1825:1838	CONCLUSIONS Glc-Fru isomers were discriminated in the PCA score plots for their lithium and sodium adducts by using different collision modes.
26467229	3	7	theme	sodium	717:722	arg1	adducts					724:730	sodium adducts	717:730	sodium adducts	717:730	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	9	8	theme	CONCLUSIONS	1733:1743	arg1	isomers					1753:1759	CONCLUSIONS Glc-Fru isomers	1733:1759	CONCLUSIONS Glc-Fru isomers	1733:1759	CONCLUSIONS Glc-Fru isomers were discriminated in the PCA score plots for their lithium and sodium adducts by using different collision modes.
26467229	11	9	theme	hydroxyl	2058:2065	arg1	group					2067:2071	the hydroxyl group	2054:2071	the hydroxyl group	2054:2071	The hydrogen migration of the hydroxyl group at C3 of the fructose unit caused the glycosidic bond decomposition of lithiated sucrose.
26467229	6	10	theme	fragment	1158:1165	arg1	ions					1167:1170	The main fragment ions	1149:1170	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1141:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	2	11	used	used	311:314	arg2	We					308:309	We	308:309	We	308:309	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	3	12	theme	glucopyranosyl	739:752	arg1	isomers					773:779	the glucopyranosyl fructose (Glc-Fru) isomers	735:779	the glucopyranosyl fructose (Glc-Fru) isomers	735:779	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	8	13	from	differences	1519:1529	arg1	patterns					1552:1559	fragmentation patterns	1538:1559	fragmentation patterns	1538:1559	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	8	13	from	differences	1519:1529	arg1	abundances					1574:1583	relative abundances	1565:1583	relative abundances	1565:1583	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	2	14	theme	component	343:351	arg1	analysis					353:360	principle component analysis	333:360	principle component analysis (PCA)	333:366	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	14	theme	component	343:351	arg1	PCA					363:365	PCA	363:365	PCA	363:365	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	1	15	theme	monosaccharide	241:254	arg1	units					256:260	monosaccharide units	241:260	monosaccharide units	241:260	RATIONALE The identification of the structure of carbohydrates is challenging because of their complex composition of monosaccharide units, linkage position and anomeric configuration.
26467229	6	16	dep	m/z	1304:1306	arg1	+					1320:1320	+	1320:1320	+	1320:1320	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	17	theme	cross-ring	1233:1242	arg1	cleavage					1244:1251	cross-ring cleavage	1233:1251	cross-ring cleavage (-90 Da)	1233:1260	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	17	theme	cross-ring	1233:1242	arg1	Da					1258:1259	-90 Da	1254:1259	-90 Da	1254:1259	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	9	18	theme	Glc-Fru	1745:1751	arg1	isomers					1753:1759	CONCLUSIONS Glc-Fru isomers	1733:1759	CONCLUSIONS Glc-Fru isomers	1733:1759	CONCLUSIONS Glc-Fru isomers were discriminated in the PCA score plots for their lithium and sodium adducts by using different collision modes.
26467229	3	19	dep	METHODS	642:648	arg1	recorded					786:793	recorded	786:793	were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap)	781:886	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	0	20	from	spectrometry	92:103	arg1	mode					117:120	positive mode	108:120	positive mode	108:120	Distinguishing isomeric aldohexose-ketohexose disaccharides by electrospray ionization mass spectrometry in positive mode.
26467229	6	21	theme	precursor	1286:1294	arg1	ions					1296:1299	the precursor ions	1282:1299	the precursor ions of m/z 349 ([M+Li](+))	1282:1322	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	8	22	theme	alkali	1697:1702	arg1	adducts					1704:1710	the alkali adducts	1693:1710	the alkali adducts	1693:1710	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	6	23	theme	glycosidic	1202:1211	arg1	decomposition					1218:1230	the glycosidic bond decomposition	1198:1230	the glycosidic bond decomposition	1198:1230	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	3	24	theme	independent	802:812	arg1	spectrometers					819:831	two independent mass spectrometers	798:831	two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap)	798:886	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	3	25	theme	MicroTOF	844:851	arg1	CID					839:841	CID	839:841	CID (MicroTOF QII)	839:856	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	3	25	theme	MicroTOF	844:851	arg1	QII					853:855	MicroTOF QII	844:855	MicroTOF QII	844:855	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	2	26	theme	aldohexose-ketohexose	521:541	arg1	maltulose					571:579	maltulose	571:579	maltulose	571:579	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	26	theme	aldohexose-ketohexose	521:541	arg1	palatinose					586:595	palatinose	586:595	palatinose	586:595	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	26	theme	aldohexose-ketohexose	521:541	arg1	turanose					561:568	turanose	561:568	turanose	561:568	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	26	theme	aldohexose-ketohexose	521:541	arg1	sucrose					552:558	sucrose	552:558	sucrose	552:558	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	26	theme	aldohexose-ketohexose	521:541	arg1	isomers					543:549	four aldohexose-ketohexose isomers	516:549	four aldohexose-ketohexose isomers	516:549	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	10	27	theme	sodium	2012:2017	arg1	adducts					2019:2025	sodium adducts	2012:2025	sodium adducts	2012:2025	The results showed that HCD-MS/MS is an ideal tool for differentiating lithium adducts, whereas, CID-MS/MS is better for discriminating sodium adducts.
26467229	5	28	theme	Gaussian	1127:1134	arg1	calculations					1113:1124	density functional theory calculations	1087:1124	density functional theory calculations (Gaussian 09)	1087:1138	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	5	28	theme	Gaussian	1127:1134	arg1	09					1136:1137	Gaussian 09	1127:1137	Gaussian 09	1127:1137	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	3	29	theme	Q-Exactive	867:876	arg1	HCD					862:864	HCD	862:864	HCD (Q-Exactive Orbitrap)	862:886	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	3	29	theme	Q-Exactive	867:876	arg1	Orbitrap					878:885	Q-Exactive Orbitrap	867:885	Q-Exactive Orbitrap	867:885	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	0	30	theme	positive	108:115	arg1	mode					117:120	positive mode	108:120	positive mode	108:120	Distinguishing isomeric aldohexose-ketohexose disaccharides by electrospray ionization mass spectrometry in positive mode.
26467229	6	31	from	m/z	1328:1330	arg1	RESULTS					1141:1147	RESULTS The main fragment ions	1141:1170	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1141:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	31	from	m/z	1328:1330	arg1	spectra					1185:1191	the MS/MS spectra	1175:1191	the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1175:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	5	32	theme	functional	1095:1104	arg1	calculations					1113:1124	density functional theory calculations	1087:1124	density functional theory calculations (Gaussian 09)	1087:1138	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	5	32	theme	functional	1095:1104	arg1	09					1136:1137	Gaussian 09	1127:1137	Gaussian 09	1127:1137	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	8	33	from	abundances	1574:1583	arg1	spectrometry					1623:1634	second-order mass spectrometry	1605:1634	second-order mass spectrometry	1605:1634	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	10	34	theme	lithium	1947:1953	arg1	adducts					1955:1961	lithium adducts	1947:1961	lithium adducts	1947:1961	The results showed that HCD-MS/MS is an ideal tool for differentiating lithium adducts, whereas, CID-MS/MS is better for discriminating sodium adducts.
26467229	11	35	theme	fructose	2086:2093	arg1	unit					2095:2098	the fructose unit	2082:2098	the fructose unit	2082:2098	The hydrogen migration of the hydroxyl group at C3 of the fructose unit caused the glycosidic bond decomposition of lithiated sucrose.
26467229	11	36	theme	unit	2095:2098	arg1	C3					2076:2077	C3	2076:2077	C3 of the fructose unit	2076:2098	The hydrogen migration of the hydroxyl group at C3 of the fructose unit caused the glycosidic bond decomposition of lithiated sucrose.
26467229	2	37	theme	energy	464:469	arg1	HCD					495:497	HCD	495:497	HCD	495:497	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	37	theme	energy	464:469	arg1	dissociation					481:492	higher energy collision dissociation	457:492	higher energy collision dissociation (HCD)	457:498	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	6	38	from	RESULTS	1141:1147	arg1	[M+Na					1337:1341	[M+Na	1337:1341	[M+Na	1337:1341	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	38	from	RESULTS	1141:1147	arg1	cleavage					1244:1251	cross-ring cleavage	1233:1251	cross-ring cleavage (-90 Da)	1233:1260	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	38	from	RESULTS	1141:1147	arg1	Da					1258:1259	-90 Da	1254:1259	-90 Da	1254:1259	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	38	from	RESULTS	1141:1147	arg1	m/z					1328:1330	m/z 365	1328:1334	m/z 365 ([M+Na](+))	1328:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	38	from	RESULTS	1141:1147	arg1	decomposition					1218:1230	the glycosidic bond decomposition	1198:1230	the glycosidic bond decomposition	1198:1230	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	38	from	RESULTS	1141:1147	arg1	spectra					1185:1191	the MS/MS spectra	1175:1191	the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1175:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	38	from	RESULTS	1141:1147	arg1	dehydration					1267:1277	dehydration	1267:1277	dehydration of the precursor ions of m/z 349 ([M+Li](+))	1267:1322	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	0	39	theme	aldohexose-ketohexose	24:44	arg1	disaccharides					46:58	isomeric aldohexose-ketohexose disaccharides	15:58	isomeric aldohexose-ketohexose disaccharides	15:58	Distinguishing isomeric aldohexose-ketohexose disaccharides by electrospray ionization mass spectrometry in positive mode.
26467229	3	40	dep	lithium	705:711	arg1	the					701:703	the	701:703	the	701:703	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	11	41	theme	glycosidic	2111:2120	arg1	decomposition					2127:2139	the glycosidic bond decomposition	2107:2139	the glycosidic bond decomposition of lithiated sucrose	2107:2160	The hydrogen migration of the hydroxyl group at C3 of the fructose unit caused the glycosidic bond decomposition of lithiated sucrose.
26467229	7	42	theme	ions	1430:1433	arg1	dehydration					1401:1411	dehydration	1401:1411	dehydration	1401:1411	Surprisingly, cross-ring cleavage and dehydration of the precursor ions were rarely observed in both lithiated and sodiated sucrose.
26467229	7	42	theme	ions	1430:1433	arg1	cleavage					1388:1395	cross-ring cleavage	1377:1395	cross-ring cleavage	1377:1395	Surprisingly, cross-ring cleavage and dehydration of the precursor ions were rarely observed in both lithiated and sodiated sucrose.
26467229	5	43	theme	lithiated	1007:1015	arg1	sucrose					1017:1023	lithiated sucrose	1007:1023	lithiated sucrose	1007:1023	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	3	44	theme	electrospray	654:665	arg1	ionization					667:676	electrospray ionization	654:676	The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers	650:779	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	3	44	theme	electrospray	654:665	arg1	ESI					679:681	ESI	679:681	ESI	679:681	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	2	45	theme	mass	379:382	arg1	MS/MS					398:402	MS/MS	398:402	MS/MS	398:402	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	45	theme	mass	379:382	arg1	spectrometry					384:395	tandem mass spectrometry	372:395	tandem mass spectrometry (MS/MS)	372:403	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	0	46	theme	ionization	76:85	arg1	spectrometry					92:103	electrospray ionization mass spectrometry	63:103	electrospray ionization mass spectrometry in positive mode	63:120	Distinguishing isomeric aldohexose-ketohexose disaccharides by electrospray ionization mass spectrometry in positive mode.
26467229	11	47	theme	sucrose	2154:2160	arg1	decomposition					2127:2139	the glycosidic bond decomposition	2107:2139	the glycosidic bond decomposition of lithiated sucrose	2107:2160	The hydrogen migration of the hydroxyl group at C3 of the fructose unit caused the glycosidic bond decomposition of lithiated sucrose.
26467229	9	48	theme	PCA	1787:1789	arg1	plots					1797:1801	the PCA score plots	1783:1801	the PCA score plots for their lithium and sodium adducts	1783:1838	CONCLUSIONS Glc-Fru isomers were discriminated in the PCA score plots for their lithium and sodium adducts by using different collision modes.
26467229	8	49	theme	relative	1565:1572	arg1	abundances					1574:1583	relative abundances	1565:1583	relative abundances	1565:1583	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	1	50	theme	linkage	263:269	arg1	position					271:278	linkage position	263:278	linkage position	263:278	RATIONALE The identification of the structure of carbohydrates is challenging because of their complex composition of monosaccharide units, linkage position and anomeric configuration.
26467229	5	51	theme	bond	980:983	arg1	mechanism					994:1002	The glycosidic bond cleavage mechanism	965:1002	The glycosidic bond cleavage mechanism of lithiated sucrose	965:1023	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	6	52	from	dehydration	1267:1277	arg1	RESULTS					1141:1147	RESULTS The main fragment ions	1141:1170	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1141:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	52	from	dehydration	1267:1277	arg1	spectra					1185:1191	the MS/MS spectra	1175:1191	the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1175:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	8	53	theme	ions	1597:1600	arg1	patterns					1552:1559	fragmentation patterns	1538:1559	fragmentation patterns	1538:1559	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	8	53	theme	ions	1597:1600	arg1	abundances					1574:1583	relative abundances	1565:1583	relative abundances	1565:1583	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	2	54	theme	principle	333:341	arg1	analysis					353:360	principle component analysis	333:360	principle component analysis (PCA)	333:366	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	54	theme	principle	333:341	arg1	PCA					363:365	PCA	363:365	PCA	363:365	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	8	55	theme	second-order	1605:1616	arg1	spectrometry					1623:1634	second-order mass spectrometry	1605:1634	second-order mass spectrometry	1605:1634	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	9	56	theme	different	1849:1857	arg1	modes					1869:1873	different collision modes	1849:1873	different collision modes	1849:1873	CONCLUSIONS Glc-Fru isomers were discriminated in the PCA score plots for their lithium and sodium adducts by using different collision modes.
26467229	6	57	dep	RESULTS	1141:1147	arg1	ions					1167:1170	The main fragment ions	1149:1170	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1141:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	3	58	theme	adducts	724:730	arg1	spectra					690:696	The electrospray ionization (ESI)-MS/MS spectra	650:696	The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers	650:779	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	1	59	theme	complex	218:224	arg1	composition					226:236	their complex composition	212:236	their complex composition of monosaccharide units	212:260	RATIONALE The identification of the structure of carbohydrates is challenging because of their complex composition of monosaccharide units, linkage position and anomeric configuration.
26467229	11	60	theme	group	2067:2071	arg1	migration					2041:2049	The hydrogen migration	2028:2049	The hydrogen migration of the hydroxyl group at C3 of the fructose unit	2028:2098	The hydrogen migration of the hydroxyl group at C3 of the fructose unit caused the glycosidic bond decomposition of lithiated sucrose.
26467229	6	61	theme	main	1153:1156	arg1	ions					1167:1170	The main fragment ions	1149:1170	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1141:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	9	62	theme	collision	1859:1867	arg1	modes					1869:1873	different collision modes	1849:1873	different collision modes	1849:1873	CONCLUSIONS Glc-Fru isomers were discriminated in the PCA score plots for their lithium and sodium adducts by using different collision modes.
26467229	3	63	theme	Glc-Fru	764:770	arg1	isomers					773:779	the glucopyranosyl fructose (Glc-Fru) isomers	735:779	the glucopyranosyl fructose (Glc-Fru) isomers	735:779	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	2	64	theme	analysis	353:360	arg1	MS/MS					398:402	MS/MS	398:402	MS/MS	398:402	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	64	theme	analysis	353:360	arg1	combination					318:328	a combination	316:328	a combination of principle component analysis (PCA)	316:366	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	64	theme	analysis	353:360	arg1	spectrometry					384:395	tandem mass spectrometry	372:395	tandem mass spectrometry (MS/MS)	372:403	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	8	65	theme	discriminant	1651:1662	arg1	models					1664:1669	discriminant models	1651:1669	discriminant models	1651:1669	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	7	66	located	observed	1447:1454	arg1	sucrose					1487:1493	both lithiated and sodiated sucrose	1459:1493	both lithiated and sodiated sucrose	1459:1493	Surprisingly, cross-ring cleavage and dehydration of the precursor ions were rarely observed in both lithiated and sodiated sucrose.
26467229	7	66	located	observed	1447:1454	arg2	cleavage					1388:1395	cross-ring cleavage	1377:1395	cross-ring cleavage	1377:1395	Surprisingly, cross-ring cleavage and dehydration of the precursor ions were rarely observed in both lithiated and sodiated sucrose.
26467229	7	66	located	observed	1447:1454	arg2	dehydration					1401:1411	dehydration	1401:1411	dehydration	1401:1411	Surprisingly, cross-ring cleavage and dehydration of the precursor ions were rarely observed in both lithiated and sodiated sucrose.
26467229	3	67	theme	fructose	754:761	arg1	isomers					773:779	the glucopyranosyl fructose (Glc-Fru) isomers	735:779	the glucopyranosyl fructose (Glc-Fru) isomers	735:779	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	6	68	theme	m/z	1304:1306	arg1	ions					1296:1299	the precursor ions	1282:1299	the precursor ions of m/z 349 ([M+Li](+))	1282:1322	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	1	69	theme	units	256:260	arg1	composition					226:236	their complex composition	212:236	their complex composition of monosaccharide units	212:260	RATIONALE The identification of the structure of carbohydrates is challenging because of their complex composition of monosaccharide units, linkage position and anomeric configuration.
26467229	1	69	theme	units	256:260	arg1	position					271:278	linkage position	263:278	linkage position	263:278	RATIONALE The identification of the structure of carbohydrates is challenging because of their complex composition of monosaccharide units, linkage position and anomeric configuration.
26467229	1	69	theme	units	256:260	arg1	configuration					293:305	anomeric configuration	284:305	anomeric configuration	284:305	RATIONALE The identification of the structure of carbohydrates is challenging because of their complex composition of monosaccharide units, linkage position and anomeric configuration.
26467229	4	70	theme	fragment	917:924	arg1	ions					926:929	the fragment ions	913:929	the fragment ions	913:929	The differences between the fragment ions were evaluated by the PCA models.
26467229	10	71	theme	ideal	1916:1920	arg1	HCD-MS/MS					1900:1908	HCD-MS/MS	1900:1908	HCD-MS/MS	1900:1908	The results showed that HCD-MS/MS is an ideal tool for differentiating lithium adducts, whereas, CID-MS/MS is better for discriminating sodium adducts.
26467229	10	71	theme	ideal	1916:1920	arg1	tool					1922:1925	an ideal tool	1913:1925	an ideal tool for differentiating lithium adducts	1913:1961	The results showed that HCD-MS/MS is an ideal tool for differentiating lithium adducts, whereas, CID-MS/MS is better for discriminating sodium adducts.
26467229	6	72	theme	ions	1296:1299	arg1	[M+Na					1337:1341	[M+Na	1337:1341	[M+Na	1337:1341	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	72	theme	ions	1296:1299	arg1	cleavage					1244:1251	cross-ring cleavage	1233:1251	cross-ring cleavage (-90 Da)	1233:1260	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	72	theme	ions	1296:1299	arg1	Da					1258:1259	-90 Da	1254:1259	-90 Da	1254:1259	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	72	theme	ions	1296:1299	arg1	m/z					1328:1330	m/z 365	1328:1334	m/z 365 ([M+Na](+))	1328:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	72	theme	ions	1296:1299	arg1	decomposition					1218:1230	the glycosidic bond decomposition	1198:1230	the glycosidic bond decomposition	1198:1230	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	72	theme	ions	1296:1299	arg1	dehydration					1267:1277	dehydration	1267:1277	dehydration of the precursor ions of m/z 349 ([M+Li](+))	1267:1322	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	5	73	theme	density	1087:1093	arg1	calculations					1113:1124	density functional theory calculations	1087:1124	density functional theory calculations (Gaussian 09)	1087:1138	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	5	73	theme	density	1087:1093	arg1	09					1136:1137	Gaussian 09	1127:1137	Gaussian 09	1127:1137	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	6	74	theme	bond	1213:1216	arg1	decomposition					1218:1230	the glycosidic bond decomposition	1198:1230	the glycosidic bond decomposition	1198:1230	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	75	from	decomposition	1218:1230	arg1	RESULTS					1141:1147	RESULTS The main fragment ions	1141:1170	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1141:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	6	75	from	decomposition	1218:1230	arg1	spectra					1185:1191	the MS/MS spectra	1175:1191	the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+))	1175:1346	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	3	76	theme	isomers	773:779	arg1	lithium					705:711	lithium	705:711	lithium	705:711	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	3	76	theme	isomers	773:779	arg1	adducts					724:730	sodium adducts	717:730	sodium adducts	717:730	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	1	77	theme	structure	159:167	arg1	identification					137:150	The identification	133:150	RATIONALE The identification of the structure of carbohydrates	123:184	RATIONALE The identification of the structure of carbohydrates is challenging because of their complex composition of monosaccharide units, linkage position and anomeric configuration.
26467229	3	78	theme	mass	814:817	arg1	spectrometers					819:831	two independent mass spectrometers	798:831	two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap)	798:886	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	5	79	theme	deuterium-labeling	1043:1060	arg1	experiment					1062:1071	a deuterium-labeling experiment	1041:1071	a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09)	1041:1138	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	1	80	theme	carbohydrates	172:184	arg1	structure					159:167	the structure	155:167	the structure of carbohydrates	155:184	RATIONALE The identification of the structure of carbohydrates is challenging because of their complex composition of monosaccharide units, linkage position and anomeric configuration.
26467229	7	81	theme	sodiated	1478:1485	arg1	sucrose					1487:1493	both lithiated and sodiated sucrose	1459:1493	both lithiated and sodiated sucrose	1459:1493	Surprisingly, cross-ring cleavage and dehydration of the precursor ions were rarely observed in both lithiated and sodiated sucrose.
26467229	4	82	theme	PCA	953:955	arg1	models					957:962	the PCA models	949:962	the PCA models	949:962	The differences between the fragment ions were evaluated by the PCA models.
26467229	8	83	dep	patterns	1552:1559	arg1	the					1534:1536	the	1534:1536	the	1534:1536	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	8	84	theme	collision	1716:1724	arg1	modes					1726:1730	collision modes	1716:1730	collision modes	1716:1730	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	7	85	theme	lithiated	1464:1472	arg1	sucrose					1487:1493	both lithiated and sodiated sucrose	1459:1493	both lithiated and sodiated sucrose	1459:1493	Surprisingly, cross-ring cleavage and dehydration of the precursor ions were rarely observed in both lithiated and sodiated sucrose.
26467229	1	86	dep	RATIONALE	123:131	arg1	identification					137:150	The identification	133:150	RATIONALE The identification of the structure of carbohydrates	123:184	RATIONALE The identification of the structure of carbohydrates is challenging because of their complex composition of monosaccharide units, linkage position and anomeric configuration.
26467229	11	87	theme	lithiated	2144:2152	arg1	sucrose					2154:2160	lithiated sucrose	2144:2160	lithiated sucrose	2144:2160	The hydrogen migration of the hydroxyl group at C3 of the fructose unit caused the glycosidic bond decomposition of lithiated sucrose.
26467229	5	88	theme	theory	1106:1111	arg1	calculations					1113:1124	density functional theory calculations	1087:1124	density functional theory calculations (Gaussian 09)	1087:1138	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	5	88	theme	theory	1106:1111	arg1	09					1136:1137	Gaussian 09	1127:1137	Gaussian 09	1127:1137	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	8	89	from	patterns	1552:1559	arg1	spectrometry					1623:1634	second-order mass spectrometry	1605:1634	second-order mass spectrometry	1605:1634	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	2	90	theme	collision-induced	416:432	arg1	CID					448:450	CID	448:450	CID	448:450	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	90	theme	collision-induced	416:432	arg1	dissociation					434:445	collision-induced dissociation	416:445	collision-induced dissociation (CID)	416:451	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	0	91	theme	isomeric	15:22	arg1	disaccharides					46:58	isomeric aldohexose-ketohexose disaccharides	15:58	isomeric aldohexose-ketohexose disaccharides	15:58	Distinguishing isomeric aldohexose-ketohexose disaccharides by electrospray ionization mass spectrometry in positive mode.
26467229	11	92	from	C3	2076:2077	arg1	migration					2041:2049	The hydrogen migration	2028:2049	The hydrogen migration of the hydroxyl group at C3 of the fructose unit	2028:2098	The hydrogen migration of the hydroxyl group at C3 of the fructose unit caused the glycosidic bond decomposition of lithiated sucrose.
26467229	6	93	dep	m/z	1328:1330	arg1	+					1344:1344	+	1344:1344	+	1344:1344	RESULTS The main fragment ions in the MS/MS spectra from the glycosidic bond decomposition, cross-ring cleavage (-90 Da), and dehydration of the precursor ions of m/z 349 ([M+Li](+)) and m/z 365 ([M+Na](+)) were observed.
26467229	2	94	theme	collision	471:479	arg1	HCD					495:497	HCD	495:497	HCD	495:497	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	94	theme	collision	471:479	arg1	dissociation					481:492	higher energy collision dissociation	457:492	higher energy collision dissociation (HCD)	457:498	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	95	theme	higher	457:462	arg1	HCD					495:497	HCD	495:497	HCD	495:497	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	95	theme	higher	457:462	arg1	dissociation					481:492	higher energy collision dissociation	457:492	higher energy collision dissociation (HCD)	457:498	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	3	96	theme	ionization	667:676	arg1	spectra					690:696	The electrospray ionization (ESI)-MS/MS spectra	650:696	The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers	650:779	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	8	97	from	ions	1597:1600	arg1	spectrometry					1623:1634	second-order mass spectrometry	1605:1634	second-order mass spectrometry	1605:1634	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	0	98	theme	electrospray	63:74	arg1	spectrometry					92:103	electrospray ionization mass spectrometry	63:103	electrospray ionization mass spectrometry in positive mode	63:120	Distinguishing isomeric aldohexose-ketohexose disaccharides by electrospray ionization mass spectrometry in positive mode.
26467229	11	99	theme	bond	2122:2125	arg1	decomposition					2127:2139	the glycosidic bond decomposition	2107:2139	the glycosidic bond decomposition of lithiated sucrose	2107:2160	The hydrogen migration of the hydroxyl group at C3 of the fructose unit caused the glycosidic bond decomposition of lithiated sucrose.
26467229	0	100	theme	mass	87:90	arg1	spectrometry					92:103	electrospray ionization mass spectrometry	63:103	electrospray ionization mass spectrometry in positive mode	63:120	Distinguishing isomeric aldohexose-ketohexose disaccharides by electrospray ionization mass spectrometry in positive mode.
26467229	5	101	theme	sucrose	1017:1023	arg1	mechanism					994:1002	The glycosidic bond cleavage mechanism	965:1002	The glycosidic bond cleavage mechanism of lithiated sucrose	965:1023	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	3	102	theme	-MS/MS	683:688	arg1	spectra					690:696	The electrospray ionization (ESI)-MS/MS spectra	650:696	The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers	650:779	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	8	103	from	spectrometry	1623:1634	arg1	patterns					1552:1559	fragmentation patterns	1538:1559	fragmentation patterns	1538:1559	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	8	103	from	spectrometry	1623:1634	arg1	abundances					1574:1583	relative abundances	1565:1583	relative abundances	1565:1583	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	2	104	theme	tandem	372:377	arg1	MS/MS					398:402	MS/MS	398:402	MS/MS	398:402	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	2	104	theme	tandem	372:377	arg1	spectrometry					384:395	tandem mass spectrometry	372:395	tandem mass spectrometry (MS/MS)	372:403	We used a combination of principle component analysis (PCA) and tandem mass spectrometry (MS/MS), including collision-induced dissociation (CID) and higher energy collision dissociation (HCD), to distinguish four aldohexose-ketohexose isomers, sucrose, turanose, maltulose, and palatinose, which are composed of glucose and fructose.
26467229	7	105	theme	precursor	1420:1428	arg1	ions					1430:1433	the precursor ions	1416:1433	the precursor ions	1416:1433	Surprisingly, cross-ring cleavage and dehydration of the precursor ions were rarely observed in both lithiated and sodiated sucrose.
26467229	5	106	theme	glycosidic	969:978	arg1	mechanism					994:1002	The glycosidic bond cleavage mechanism	965:1002	The glycosidic bond cleavage mechanism of lithiated sucrose	965:1023	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	9	107	theme	score	1791:1795	arg1	plots					1797:1801	the PCA score plots	1783:1801	the PCA score plots for their lithium and sodium adducts	1783:1838	CONCLUSIONS Glc-Fru isomers were discriminated in the PCA score plots for their lithium and sodium adducts by using different collision modes.
26467229	8	108	theme	significant	1507:1517	arg1	differences					1519:1529	significant differences	1507:1529	significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes	1507:1730	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	8	109	theme	fragment	1588:1595	arg1	ions					1597:1600	fragment ions	1588:1600	fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes	1588:1730	There were significant differences in the fragmentation patterns and relative abundances of fragment ions in second-order mass spectrometry, which allowed discriminant models to be constructed for the alkali adducts and collision modes.
26467229	5	110	theme	cleavage	985:992	arg1	mechanism					994:1002	The glycosidic bond cleavage mechanism	965:1002	The glycosidic bond cleavage mechanism of lithiated sucrose	965:1023	The glycosidic bond cleavage mechanism of lithiated sucrose was verified by a deuterium-labeling experiment combined with density functional theory calculations (Gaussian 09).
26467229	3	111	theme	lithium	705:711	arg1	spectra					690:696	The electrospray ionization (ESI)-MS/MS spectra	650:696	The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers	650:779	METHODS The electrospray ionization (ESI)-MS/MS spectra of the lithium and sodium adducts of the glucopyranosyl fructose (Glc-Fru) isomers were recorded on two independent mass spectrometers using CID (MicroTOF QII) and HCD (Q-Exactive Orbitrap).
26467229	1	112	theme	anomeric	284:291	arg1	configuration					293:305	anomeric configuration	284:305	anomeric configuration	284:305	RATIONALE The identification of the structure of carbohydrates is challenging because of their complex composition of monosaccharide units, linkage position and anomeric configuration.
28038742	0	0	theme	improved	80:87	arg1	healing					100:106	an improved skin wound healing	77:106	an improved skin wound healing	77:106	Ibuprofen loaded PVA/chitosan membranes: A highly efficient strategy towards an improved skin wound healing.
28038742	3	1	dep	/chitosan	618:626	arg1	alcohol					610:616	vinyl alcohol	604:616	vinyl alcohol	604:616	Herein, IBP-β-cyclodextrins carriers were designed to customise the release profile of IBP from poly(vinyl alcohol)/chitosan (PVA/CS) dressings in order to promote a faster skin regeneration.
28038742	3	1	dep	/chitosan	618:626	arg1	PVA/CS					629:634	PVA/CS	629:634	PVA/CS	629:634	Herein, IBP-β-cyclodextrins carriers were designed to customise the release profile of IBP from poly(vinyl alcohol)/chitosan (PVA/CS) dressings in order to promote a faster skin regeneration.
28038742	6	2	contain	containing	1032:1041	arg2	carriers					1063:1070	IBP-β-cyclodextrins carriers	1043:1070	IBP-β-cyclodextrins carriers	1043:1070	Moreover, the in vivo assays revealed that the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers avoided scab formation and an excessive inflammation, enabling an earlier skin healing.
28038742	6	2	contain	containing	1032:1041	arg1	membranes					1022:1030	PVA/CS membranes	1015:1030	PVA/CS membranes containing IBP-β-cyclodextrins carriers	1015:1070	Moreover, the in vivo assays revealed that the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers avoided scab formation and an excessive inflammation, enabling an earlier skin healing.
28038742	0	3	theme	wound	94:98	arg1	healing					100:106	an improved skin wound healing	77:106	an improved skin wound healing	77:106	Ibuprofen loaded PVA/chitosan membranes: A highly efficient strategy towards an improved skin wound healing.
28038742	5	4	from	hydrogels	886:894	arg1	release					869:875	a controlled drug release	851:875	a controlled drug release from the hydrogels which is crucial for their application in wound management	851:953	In vitro IBP release studies showed that β-cyclodextrins allowed a controlled drug release from the hydrogels which is crucial for their application in wound management.
28038742	0	5	theme	skin	89:92	arg1	healing					100:106	an improved skin wound healing	77:106	an improved skin wound healing	77:106	Ibuprofen loaded PVA/chitosan membranes: A highly efficient strategy towards an improved skin wound healing.
28038742	6	6	theme	membranes	1022:1030	arg1	presence					1003:1010	the presence	999:1010	the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers	999:1070	Moreover, the in vivo assays revealed that the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers avoided scab formation and an excessive inflammation, enabling an earlier skin healing.
28038742	2	7	theme	wound	447:451	arg1	healing					453:459	skin wound healing	442:459	skin wound healing	442:459	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	6	8	theme	excessive	1102:1110	arg1	inflammation					1112:1123	an excessive inflammation	1099:1123	an excessive inflammation	1099:1123	Moreover, the in vivo assays revealed that the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers avoided scab formation and an excessive inflammation, enabling an earlier skin healing.
28038742	5	9	from	application	923:933	arg1	management					944:953	wound management	938:953	wound management	938:953	In vitro IBP release studies showed that β-cyclodextrins allowed a controlled drug release from the hydrogels which is crucial for their application in wound management.
28038742	2	10	theme	skin	442:445	arg1	healing					453:459	skin wound healing	442:459	skin wound healing	442:459	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	5	11	theme	controlled	853:862	arg1	release					869:875	a controlled drug release	851:875	a controlled drug release from the hydrogels which is crucial for their application in wound management	851:953	In vitro IBP release studies showed that β-cyclodextrins allowed a controlled drug release from the hydrogels which is crucial for their application in wound management.
28038742	4	12	dep	-assisted	765:773	arg1	dioxide					750:756	supercritical carbon dioxide	729:756	supercritical carbon dioxide	729:756	The dressings were produced using supercritical carbon dioxide (scCO2)-assisted technique.
28038742	4	13	theme	-assisted	765:773	arg1	technique					775:783	supercritical carbon dioxide (scCO2)-assisted technique	729:783	supercritical carbon dioxide (scCO2)-assisted technique	729:783	The dressings were produced using supercritical carbon dioxide (scCO2)-assisted technique.
28038742	2	14	theme	S	298:298	arg1	IBP					312:314	IBP	312:314	IBP	312:314	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	2	14	theme	S	298:298	arg1	-ibuprofen					300:309	(S)-ibuprofen	297:309	(S)-ibuprofen (IBP)	297:315	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	2	14	theme	S	298:298	arg1	agent					352:356	a non-steroidal anti-inflammatory agent	318:356	a non-steroidal anti-inflammatory agent	318:356	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	5	15	theme	In	786:787	arg1	studies					807:813	In vitro IBP release studies	786:813	In vitro IBP release studies	786:813	In vitro IBP release studies showed that β-cyclodextrins allowed a controlled drug release from the hydrogels which is crucial for their application in wound management.
28038742	5	16	theme	drug	864:867	arg1	release					869:875	a controlled drug release	851:875	a controlled drug release from the hydrogels which is crucial for their application in wound management	851:953	In vitro IBP release studies showed that β-cyclodextrins allowed a controlled drug release from the hydrogels which is crucial for their application in wound management.
28038742	2	17	theme	anti-inflammatory	334:350	arg1	agent					352:356	a non-steroidal anti-inflammatory agent	318:356	a non-steroidal anti-inflammatory agent	318:356	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	2	17	theme	anti-inflammatory	334:350	arg1	-ibuprofen					300:309	(S)-ibuprofen	297:309	(S)-ibuprofen (IBP)	297:315	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	1	18	theme	disproportionate	250:265	arg1	response					280:287	a disproportionate inflammatory response	248:287	a disproportionate inflammatory response	248:287	During wound healing, an early inflammation can cause an increase of the wound size and the healing process can be considerably belated if a disproportionate inflammatory response occurs.
28038742	4	19	theme	carbon	743:748	arg1	dioxide					750:756	supercritical carbon dioxide	729:756	supercritical carbon dioxide	729:756	The dressings were produced using supercritical carbon dioxide (scCO2)-assisted technique.
28038742	6	20	theme	scab	1080:1083	arg1	formation					1085:1093	scab formation	1080:1093	scab formation	1080:1093	Moreover, the in vivo assays revealed that the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers avoided scab formation and an excessive inflammation, enabling an earlier skin healing.
28038742	5	21	dep	In	786:787	arg1	vitro					789:793	vitro	789:793	vitro	789:793	In vitro IBP release studies showed that β-cyclodextrins allowed a controlled drug release from the hydrogels which is crucial for their application in wound management.
28038742	2	22	theme	muscle	377:382	arg1	healing					384:390	muscle healing	377:390	muscle healing	377:390	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	0	23	theme	PVA/chitosan	17:28	arg1	membranes					30:38	PVA/chitosan membranes	17:38	PVA/chitosan membranes	17:38	Ibuprofen loaded PVA/chitosan membranes: A highly efficient strategy towards an improved skin wound healing.
28038742	1	24	theme	inflammatory	267:278	arg1	response					280:287	a disproportionate inflammatory response	248:287	a disproportionate inflammatory response	248:287	During wound healing, an early inflammation can cause an increase of the wound size and the healing process can be considerably belated if a disproportionate inflammatory response occurs.
28038742	6	25	dep	in	970:971	arg1	vivo					973:976	vivo	973:976	vivo	973:976	Moreover, the in vivo assays revealed that the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers avoided scab formation and an excessive inflammation, enabling an earlier skin healing.
28038742	1	26	theme	wound	182:186	arg1	size					188:191	the wound size	178:191	the wound size	178:191	During wound healing, an early inflammation can cause an increase of the wound size and the healing process can be considerably belated if a disproportionate inflammatory response occurs.
28038742	6	27	theme	PVA/CS	1015:1020	arg1	membranes					1022:1030	PVA/CS membranes	1015:1030	PVA/CS membranes containing IBP-β-cyclodextrins carriers	1015:1070	Moreover, the in vivo assays revealed that the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers avoided scab formation and an excessive inflammation, enabling an earlier skin healing.
28038742	6	28	theme	IBP-β-cyclodextrins	1043:1061	arg1	carriers					1063:1070	IBP-β-cyclodextrins carriers	1043:1070	IBP-β-cyclodextrins carriers	1043:1070	Moreover, the in vivo assays revealed that the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers avoided scab formation and an excessive inflammation, enabling an earlier skin healing.
28038742	3	29	theme	IBP	590:592	arg1	profile					579:585	the release profile	567:585	the release profile of IBP	567:592	Herein, IBP-β-cyclodextrins carriers were designed to customise the release profile of IBP from poly(vinyl alcohol)/chitosan (PVA/CS) dressings in order to promote a faster skin regeneration.
28038742	4	30	theme	supercritical	729:741	arg1	dioxide					750:756	supercritical carbon dioxide	729:756	supercritical carbon dioxide	729:756	The dressings were produced using supercritical carbon dioxide (scCO2)-assisted technique.
28038742	1	31	theme	size	188:191	arg1	increase					166:173	an increase	163:173	an increase of the wound size	163:191	During wound healing, an early inflammation can cause an increase of the wound size and the healing process can be considerably belated if a disproportionate inflammatory response occurs.
28038742	3	32	theme	release	571:577	arg1	profile					579:585	the release profile	567:585	the release profile of IBP	567:592	Herein, IBP-β-cyclodextrins carriers were designed to customise the release profile of IBP from poly(vinyl alcohol)/chitosan (PVA/CS) dressings in order to promote a faster skin regeneration.
28038742	5	33	theme	wound	938:942	arg1	management					944:953	wound management	938:953	wound management	938:953	In vitro IBP release studies showed that β-cyclodextrins allowed a controlled drug release from the hydrogels which is crucial for their application in wound management.
28038742	2	34	theme	leg	412:414	arg1	ulcers					416:421	venous leg ulcers	405:421	venous leg ulcers	405:421	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	5	35	theme	IBP	795:797	arg1	studies					807:813	In vitro IBP release studies	786:813	In vitro IBP release studies	786:813	In vitro IBP release studies showed that β-cyclodextrins allowed a controlled drug release from the hydrogels which is crucial for their application in wound management.
28038742	2	36	from	effect	432:437	arg1	healing					453:459	skin wound healing	442:459	skin wound healing	442:459	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	2	37	used	used	368:371	arg2	IBP					312:314	IBP	312:314	IBP	312:314	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	2	37	used	used	368:371	arg2	agent					352:356	a non-steroidal anti-inflammatory agent	318:356	a non-steroidal anti-inflammatory agent	318:356	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	2	37	used	used	368:371	arg2	-ibuprofen					300:309	(S)-ibuprofen	297:309	(S)-ibuprofen (IBP)	297:315	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	2	38	theme	venous	405:410	arg1	ulcers					416:421	venous leg ulcers	405:421	venous leg ulcers	405:421	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	3	39	theme	faster	669:674	arg1	regeneration					681:692	a faster skin regeneration	667:692	a faster skin regeneration	667:692	Herein, IBP-β-cyclodextrins carriers were designed to customise the release profile of IBP from poly(vinyl alcohol)/chitosan (PVA/CS) dressings in order to promote a faster skin regeneration.
28038742	3	40	theme	IBP-β-cyclodextrins	511:529	arg1	carriers					531:538	IBP-β-cyclodextrins carriers	511:538	IBP-β-cyclodextrins carriers	511:538	Herein, IBP-β-cyclodextrins carriers were designed to customise the release profile of IBP from poly(vinyl alcohol)/chitosan (PVA/CS) dressings in order to promote a faster skin regeneration.
28038742	1	41	theme	wound	116:120	arg1	healing					122:128	wound healing	116:128	wound healing	116:128	During wound healing, an early inflammation can cause an increase of the wound size and the healing process can be considerably belated if a disproportionate inflammatory response occurs.
28038742	3	42	theme	skin	676:679	arg1	regeneration					681:692	a faster skin regeneration	667:692	a faster skin regeneration	667:692	Herein, IBP-β-cyclodextrins carriers were designed to customise the release profile of IBP from poly(vinyl alcohol)/chitosan (PVA/CS) dressings in order to promote a faster skin regeneration.
28038742	3	43	theme	vinyl	604:608	arg1	alcohol					610:616	vinyl alcohol	604:616	vinyl alcohol	604:616	Herein, IBP-β-cyclodextrins carriers were designed to customise the release profile of IBP from poly(vinyl alcohol)/chitosan (PVA/CS) dressings in order to promote a faster skin regeneration.
28038742	6	44	theme	skin	1146:1149	arg1	healing					1151:1157	an earlier skin healing	1135:1157	an earlier skin healing	1135:1157	Moreover, the in vivo assays revealed that the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers avoided scab formation and an excessive inflammation, enabling an earlier skin healing.
28038742	6	45	theme	earlier	1138:1144	arg1	healing					1151:1157	an earlier skin healing	1135:1157	an earlier skin healing	1135:1157	Moreover, the in vivo assays revealed that the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers avoided scab formation and an excessive inflammation, enabling an earlier skin healing.
28038742	5	46	theme	release	799:805	arg1	studies					807:813	In vitro IBP release studies	786:813	In vitro IBP release studies	786:813	In vitro IBP release studies showed that β-cyclodextrins allowed a controlled drug release from the hydrogels which is crucial for their application in wound management.
28038742	1	47	theme	healing	201:207	arg1	process					209:215	the healing process	197:215	the healing process	197:215	During wound healing, an early inflammation can cause an increase of the wound size and the healing process can be considerably belated if a disproportionate inflammatory response occurs.
28038742	6	48	theme	in	970:971	arg1	assays					978:983	the in vivo assays	966:983	the in vivo assays	966:983	Moreover, the in vivo assays revealed that the presence of PVA/CS membranes containing IBP-β-cyclodextrins carriers avoided scab formation and an excessive inflammation, enabling an earlier skin healing.
28038742	2	49	theme	non-steroidal	320:332	arg1	agent					352:356	a non-steroidal anti-inflammatory agent	318:356	a non-steroidal anti-inflammatory agent	318:356	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	2	49	theme	non-steroidal	320:332	arg1	-ibuprofen					300:309	(S)-ibuprofen	297:309	(S)-ibuprofen (IBP)	297:315	(S)-ibuprofen (IBP), a non-steroidal anti-inflammatory agent, has been used for muscle healing and to treat venous leg ulcers, but its effect in skin wound healing has not been thoroughly studied thus far.
28038742	0	50	theme	efficient	50:58	arg1	strategy					60:67	A highly efficient strategy	41:67	A highly efficient strategy towards an improved skin wound healing	41:106	Ibuprofen loaded PVA/chitosan membranes: A highly efficient strategy towards an improved skin wound healing.
28038742	3	51	theme	/chitosan	618:626	arg1	dressings					637:645	poly(vinyl alcohol)/chitosan (PVA/CS) dressings	599:645	poly(vinyl alcohol)/chitosan (PVA/CS) dressings	599:645	Herein, IBP-β-cyclodextrins carriers were designed to customise the release profile of IBP from poly(vinyl alcohol)/chitosan (PVA/CS) dressings in order to promote a faster skin regeneration.
28038742	1	52	theme	early	134:138	arg1	inflammation					140:151	an early inflammation	131:151	an early inflammation	131:151	During wound healing, an early inflammation can cause an increase of the wound size and the healing process can be considerably belated if a disproportionate inflammatory response occurs.
26080104	0	0	theme	remained	87:94	arg1	complexes					127:135	the remained xylan and lignin-carbohydrates complexes	83:135	the remained xylan and lignin-carbohydrates complexes	83:135	Facilitating the enzymatic saccharification of pulped bamboo residues by degrading the remained xylan and lignin-carbohydrates complexes.
26080104	4	1	theme	FPU/g	693:697	arg1	cellulase					699:707	20 FPU/g cellulase	690:707	20 FPU/g cellulase	690:707	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	6	2	from	xylan	1065:1069	arg1	pulp					1087:1090	the pulp	1083:1090	the pulp	1083:1090	The results indicated that degrading the remained xylan and LCCs in the pulp could improve its enzymatic digestibility.
26080104	1	3	theme	bamboo	169:174	arg1	residues					176:183	bamboo residues	169:183	bamboo residues	169:183	Kraft pulping was performed on bamboo residues and its impact on the chemical compositions and the enzymatic digestibility of the samples were investigated.
26080104	2	4	theme	lignin-carbohydrates	361:380	arg1	complexes					382:390	the xylan and lignin-carbohydrates complexes	347:390	the xylan and lignin-carbohydrates complexes (LCCs)	347:397	To improve the digestibility of sample by degrading the xylan and lignin-carbohydrates complexes (LCCs), xylanase and α-L-arabinofuranosidase (AF) were supplemented with cellulase.
26080104	2	4	theme	lignin-carbohydrates	361:380	arg1	LCCs					393:396	LCCs	393:396	LCCs	393:396	To improve the digestibility of sample by degrading the xylan and lignin-carbohydrates complexes (LCCs), xylanase and α-L-arabinofuranosidase (AF) were supplemented with cellulase.
26080104	1	5	theme	samples	268:274	arg1	digestibility					247:259	the enzymatic digestibility	233:259	the enzymatic digestibility of the samples	233:274	Kraft pulping was performed on bamboo residues and its impact on the chemical compositions and the enzymatic digestibility of the samples were investigated.
26080104	5	6	with	saccharification	978:993	arg1	enzymes					1006:1012	these enzymes	1000:1012	these enzymes	1000:1012	The amount of LCCs in this sample decreased from 8.63/100C9 to 2.99/100C9 after saccharification with these enzymes.
26080104	4	7	theme	%	766:766	arg1	charge					771:776	12% EA charge	764:776	12% EA charge	764:776	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	4	8	theme	enzymatic	837:845	arg1	efficiency					864:873	an increased enzymatic saccharification efficiency	824:873	an increased enzymatic saccharification efficiency from 58.98% to 83.23%	824:895	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	1	9	from	residues	176:183	arg1	compositions					216:227	the chemical compositions	203:227	the chemical compositions	203:227	Kraft pulping was performed on bamboo residues and its impact on the chemical compositions and the enzymatic digestibility of the samples were investigated.
26080104	3	10	theme	effective	559:567	arg1	charge					581:586	low effective alkali (EA) charge	555:586	low effective alkali (EA) charge	555:586	The results showed more carbohydrates were remained in the samples pulped with low effective alkali (EA) charge, compared to conventional kraft pulping.
26080104	5	11	theme	LCCs	912:915	arg1	amount					902:907	The amount	898:907	The amount of LCCs in this sample	898:930	The amount of LCCs in this sample decreased from 8.63/100C9 to 2.99/100C9 after saccharification with these enzymes.
26080104	5	11	theme	LCCs	912:915	arg1	LCCs					912:915	LCCs	912:915	LCCs	912:915	The amount of LCCs in this sample decreased from 8.63/100C9 to 2.99/100C9 after saccharification with these enzymes.
26080104	0	12	theme	xylan	96:100	arg1	complexes					127:135	the remained xylan and lignin-carbohydrates complexes	83:135	the remained xylan and lignin-carbohydrates complexes	83:135	Facilitating the enzymatic saccharification of pulped bamboo residues by degrading the remained xylan and lignin-carbohydrates complexes.
26080104	4	13	theme	sample	745:750	arg1	yield					732:736	the xylan degradation yield	710:736	the xylan degradation yield of the sample pulped with 12% EA charge	710:776	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	3	14	theme	alkali	569:574	arg1	charge					581:586	low effective alkali (EA) charge	555:586	low effective alkali (EA) charge	555:586	The results showed more carbohydrates were remained in the samples pulped with low effective alkali (EA) charge, compared to conventional kraft pulping.
26080104	6	15	theme	remained	1056:1063	arg1	xylan					1065:1069	the remained xylan	1052:1069	the remained xylan	1052:1069	The results indicated that degrading the remained xylan and LCCs in the pulp could improve its enzymatic digestibility.
26080104	4	16	dep	%	798:798	arg1	to					800:801	to	800:801	to	800:801	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	4	17	theme	12	764:765	arg1	%					766:766	%	766:766	%	766:766	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	4	18	theme	IU/g	659:662	arg1	AF					664:665	15 IU/g AF	656:665	15 IU/g AF	656:665	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	0	19	theme	lignin-carbohydrates	106:125	arg1	complexes					127:135	the remained xylan and lignin-carbohydrates complexes	83:135	the remained xylan and lignin-carbohydrates complexes	83:135	Facilitating the enzymatic saccharification of pulped bamboo residues by degrading the remained xylan and lignin-carbohydrates complexes.
26080104	4	20	theme	88.35	803:807	arg1	%					798:798	%	798:798	%	798:798	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	3	21	theme	low	555:557	arg1	charge					581:586	low effective alkali (EA) charge	555:586	low effective alkali (EA) charge	555:586	The results showed more carbohydrates were remained in the samples pulped with low effective alkali (EA) charge, compared to conventional kraft pulping.
26080104	6	22	theme	enzymatic	1110:1118	arg1	digestibility					1120:1132	its enzymatic digestibility	1106:1132	its enzymatic digestibility	1106:1132	The results indicated that degrading the remained xylan and LCCs in the pulp could improve its enzymatic digestibility.
26080104	5	23	dep	2.99/100C9	961:970	arg1	to					958:959	to	958:959	to	958:959	The amount of LCCs in this sample decreased from 8.63/100C9 to 2.99/100C9 after saccharification with these enzymes.
26080104	2	24	theme	sample	327:332	arg1	digestibility					310:322	the digestibility	306:322	the digestibility of sample	306:332	To improve the digestibility of sample by degrading the xylan and lignin-carbohydrates complexes (LCCs), xylanase and α-L-arabinofuranosidase (AF) were supplemented with cellulase.
26080104	4	25	theme	degradation	720:730	arg1	yield					732:736	the xylan degradation yield	710:736	the xylan degradation yield of the sample pulped with 12% EA charge	710:776	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	4	26	theme	IU/g	638:641	arg1	xylanase					643:650	120 IU/g xylanase	634:650	120 IU/g xylanase	634:650	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	0	27	theme	enzymatic	17:25	arg1	saccharification					27:42	the enzymatic saccharification	13:42	the enzymatic saccharification of pulped bamboo residues	13:68	Facilitating the enzymatic saccharification of pulped bamboo residues by degrading the remained xylan and lignin-carbohydrates complexes.
26080104	4	28	from	%	895:895	arg1	efficiency					864:873	an increased enzymatic saccharification efficiency	824:873	an increased enzymatic saccharification efficiency from 58.98% to 83.23%	824:895	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	3	29	theme	EA	577:578	arg1	charge					581:586	low effective alkali (EA) charge	555:586	low effective alkali (EA) charge	555:586	The results showed more carbohydrates were remained in the samples pulped with low effective alkali (EA) charge, compared to conventional kraft pulping.
26080104	1	30	theme	chemical	207:214	arg1	compositions					216:227	the chemical compositions	203:227	the chemical compositions	203:227	Kraft pulping was performed on bamboo residues and its impact on the chemical compositions and the enzymatic digestibility of the samples were investigated.
26080104	0	31	theme	pulped	47:52	arg1	residues					61:68	pulped bamboo residues	47:68	pulped bamboo residues	47:68	Facilitating the enzymatic saccharification of pulped bamboo residues by degrading the remained xylan and lignin-carbohydrates complexes.
26080104	2	32	theme	xylan	351:355	arg1	complexes					382:390	the xylan and lignin-carbohydrates complexes	347:390	the xylan and lignin-carbohydrates complexes (LCCs)	347:397	To improve the digestibility of sample by degrading the xylan and lignin-carbohydrates complexes (LCCs), xylanase and α-L-arabinofuranosidase (AF) were supplemented with cellulase.
26080104	2	32	theme	xylan	351:355	arg1	LCCs					393:396	LCCs	393:396	LCCs	393:396	To improve the digestibility of sample by degrading the xylan and lignin-carbohydrates complexes (LCCs), xylanase and α-L-arabinofuranosidase (AF) were supplemented with cellulase.
26080104	4	33	theme	68.20	793:797	arg1	%					798:798	%	798:798	%	798:798	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	4	34	theme	83.23	890:894	arg1	%					885:885	%	885:885	%	885:885	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	3	35	theme	conventional	601:612	arg1	pulping					620:626	conventional kraft pulping	601:626	conventional kraft pulping	601:626	The results showed more carbohydrates were remained in the samples pulped with low effective alkali (EA) charge, compared to conventional kraft pulping.
26080104	4	36	dep	%	885:885	arg1	to					887:888	to	887:888	to	887:888	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	0	37	theme	residues	61:68	arg1	saccharification					27:42	the enzymatic saccharification	13:42	the enzymatic saccharification of pulped bamboo residues	13:68	Facilitating the enzymatic saccharification of pulped bamboo residues by degrading the remained xylan and lignin-carbohydrates complexes.
26080104	6	38	from	LCCs	1075:1078	arg1	pulp					1087:1090	the pulp	1083:1090	the pulp	1083:1090	The results indicated that degrading the remained xylan and LCCs in the pulp could improve its enzymatic digestibility.
26080104	0	39	theme	bamboo	54:59	arg1	residues					61:68	pulped bamboo residues	47:68	pulped bamboo residues	47:68	Facilitating the enzymatic saccharification of pulped bamboo residues by degrading the remained xylan and lignin-carbohydrates complexes.
26080104	4	40	theme	saccharification	847:862	arg1	efficiency					864:873	an increased enzymatic saccharification efficiency	824:873	an increased enzymatic saccharification efficiency from 58.98% to 83.23%	824:895	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	1	41	theme	Kraft	138:142	arg1	pulping					144:150	Kraft pulping	138:150	Kraft pulping	138:150	Kraft pulping was performed on bamboo residues and its impact on the chemical compositions and the enzymatic digestibility of the samples were investigated.
26080104	5	42	from	amount	902:907	arg1	sample					925:930	this sample	920:930	this sample	920:930	The amount of LCCs in this sample decreased from 8.63/100C9 to 2.99/100C9 after saccharification with these enzymes.
26080104	4	43	theme	58.98	880:884	arg1	%					885:885	%	885:885	%	885:885	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	4	44	theme	EA	768:769	arg1	charge					771:776	12% EA charge	764:776	12% EA charge	764:776	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	3	45	theme	more	495:498	arg1	carbohydrates					500:512	more carbohydrates	495:512	more carbohydrates	495:512	The results showed more carbohydrates were remained in the samples pulped with low effective alkali (EA) charge, compared to conventional kraft pulping.
26080104	3	46	dep	remained	519:526	arg1	compared					589:596	compared	589:596	compared to conventional kraft pulping	589:626	The results showed more carbohydrates were remained in the samples pulped with low effective alkali (EA) charge, compared to conventional kraft pulping.
26080104	4	47	theme	xylan	714:718	arg1	yield					732:736	the xylan degradation yield	710:736	the xylan degradation yield of the sample pulped with 12% EA charge	710:776	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	1	48	from	impact	193:198	arg1	compositions					216:227	the chemical compositions	203:227	the chemical compositions	203:227	Kraft pulping was performed on bamboo residues and its impact on the chemical compositions and the enzymatic digestibility of the samples were investigated.
26080104	4	49	theme	increased	827:835	arg1	efficiency					864:873	an increased enzymatic saccharification efficiency	824:873	an increased enzymatic saccharification efficiency from 58.98% to 83.23%	824:895	When 120 IU/g xylanase and 15 IU/g AF were supplemented with 20 FPU/g cellulase, the xylan degradation yield of the sample pulped with 12% EA charge increased from 68.20% to 88.35%, resulting in an increased enzymatic saccharification efficiency from 58.98% to 83.23%.
26080104	1	50	theme	enzymatic	237:245	arg1	digestibility					247:259	the enzymatic digestibility	233:259	the enzymatic digestibility of the samples	233:274	Kraft pulping was performed on bamboo residues and its impact on the chemical compositions and the enzymatic digestibility of the samples were investigated.
26080104	3	51	theme	kraft	614:618	arg1	pulping					620:626	conventional kraft pulping	601:626	conventional kraft pulping	601:626	The results showed more carbohydrates were remained in the samples pulped with low effective alkali (EA) charge, compared to conventional kraft pulping.
26080104	3	52	dep	showed	488:493	arg1	remained					519:526	remained	519:526	remained	519:526	The results showed more carbohydrates were remained in the samples pulped with low effective alkali (EA) charge, compared to conventional kraft pulping.
26700239	6	0	theme	type-I	833:838	arg1	staining					859:866	collagen type-I immunofluorescence staining	824:866	collagen type-I immunofluorescence staining	824:866	At 7, 14, 42, and 70 days after transplantation, frozen sections were stained with hematoxylin and eosin, followed by DAPI, β-actin, and collagen type-I immunofluorescence staining, and observed using laser confocal microscopy.
26700239	2	1	theme	calcium	310:316	arg1	alginate					318:325	calcium alginate	310:325	calcium alginate	310:325	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	10	2	theme	good	1195:1198	arg1	safety					1211:1216	good biological safety	1195:1216	good biological safety	1195:1216	The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples.
26700239	10	3	theme	subcutaneous	1244:1255	arg1	structure					1257:1265	the subcutaneous structure	1240:1265	the subcutaneous structure of the samples	1240:1280	The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples.
26700239	1	4	contain	carry	104:108	arg1	composite					81:89	A honeycomb composite	69:89	A honeycomb composite	69:89	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	1	4	contain	carry	104:108	arg1	useful					94:99	useful	94:99	useful	94:99	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	1	4	contain	carry	104:108	arg2	cells					110:114	cells	110:114	cells for application in bone, cartilage, skin, and soft tissue regenerative therapies	110:195	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	11	5	dep	control	1400:1406	arg1	rates					1431:1435	chemical reaction rates	1413:1435	chemical reaction rates	1413:1435	Freezing the gel mixture afforded control over chemical reaction rates.
26700239	6	6	theme	frozen	736:741	arg1	sections					743:750	frozen sections	736:750	frozen sections	736:750	At 7, 14, 42, and 70 days after transplantation, frozen sections were stained with hematoxylin and eosin, followed by DAPI, β-actin, and collagen type-I immunofluorescence staining, and observed using laser confocal microscopy.
26700239	3	7	theme	dilute	509:514	arg1	acid					529:532	dilute hydrochloric acid	509:532	dilute hydrochloric acid	509:532	The mixture was frozen and treated with dilute hydrochloric acid to generate a shell matrix/calcium alginate composite.
26700239	10	8	theme	control	1349:1355	arg1	samples					1357:1363	the control samples	1345:1363	the control samples	1345:1363	The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples.
26700239	1	9	from	application	120:130	arg1	skin					152:155	skin	152:155	skin	152:155	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	1	9	from	application	120:130	arg1	therapies					187:195	soft tissue regenerative therapies	162:195	soft tissue regenerative therapies	162:195	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	1	9	from	application	120:130	arg1	bone					135:138	bone	135:138	bone	135:138	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	1	9	from	application	120:130	arg1	cartilage					141:149	cartilage	141:149	cartilage	141:149	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	10	10	theme	70-day	1225:1230	arg1	period					1232:1237	a 70-day period	1223:1237	a 70-day period	1223:1237	The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples.
26700239	8	11	dep	control	965:971	arg1	The					961:963	The	961:963	The	961:963	The control and composite samples displayed significantly different mechanical properties.
26700239	8	11	dep	control	965:971	arg1	samples					987:993	samples	987:993	samples	987:993	The control and composite samples displayed significantly different mechanical properties.
26700239	2	12	theme	shell	377:381	arg1	powder					383:388	Anodonta woodiana shell powder	359:388	Anodonta woodiana shell powder	359:388	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	2	13	theme	gel	456:458	arg1	mixture					460:466	a gel mixture	454:466	a gel mixture	454:466	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	2	14	theme	mass	432:435	arg1	ratios					437:442	varying mass ratios	424:442	varying mass ratios	424:442	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	0	15	theme	honeycomb	2:10	arg1	composite					12:20	A honeycomb composite	0:20	A honeycomb composite of mollusca shell matrix and calcium alginate.	0:67	A honeycomb composite of mollusca shell matrix and calcium alginate.
26700239	0	15	theme	honeycomb	2:10	arg1	mollusca					25:32	mollusca	25:32	mollusca	25:32	A honeycomb composite of mollusca shell matrix and calcium alginate.
26700239	8	16	theme	different	1019:1027	arg1	properties					1040:1049	significantly different mechanical properties	1005:1049	significantly different mechanical properties	1005:1049	The control and composite samples displayed significantly different mechanical properties.
26700239	1	17	theme	honeycomb	71:79	arg1	composite					81:89	A honeycomb composite	69:89	A honeycomb composite	69:89	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	1	17	theme	honeycomb	71:79	arg1	useful					94:99	useful	94:99	useful	94:99	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	11	18	theme	chemical	1413:1420	arg1	rates					1431:1435	chemical reaction rates	1413:1435	chemical reaction rates	1413:1435	Freezing the gel mixture afforded control over chemical reaction rates.
26700239	2	19	theme	engineering	337:347	arg1	research					349:356	tissue engineering research	330:356	tissue engineering research	330:356	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	6	20	theme	collagen	824:831	arg1	staining					859:866	collagen type-I immunofluorescence staining	824:866	collagen type-I immunofluorescence staining	824:866	At 7, 14, 42, and 70 days after transplantation, frozen sections were stained with hematoxylin and eosin, followed by DAPI, β-actin, and collagen type-I immunofluorescence staining, and observed using laser confocal microscopy.
26700239	3	21	theme	hydrochloric	516:527	arg1	acid					529:532	dilute hydrochloric acid	509:532	dilute hydrochloric acid	509:532	The mixture was frozen and treated with dilute hydrochloric acid to generate a shell matrix/calcium alginate composite.
26700239	9	22	theme	water	1056:1060	arg1	%					1138:1138	205-496%	1131:1138	205-496%	1131:1138	The water absorption rate of the composite and control group were respectively 205-496% and 417-586%.
26700239	9	22	theme	water	1056:1060	arg1	rate					1073:1076	The water absorption rate	1052:1076	The water absorption rate of the composite and control group	1052:1111	The water absorption rate of the composite and control group were respectively 205-496% and 417-586%.
26700239	2	23	theme	tissue	330:335	arg1	engineering					337:347	tissue engineering	330:347	tissue engineering research	330:356	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	6	24	theme	confocal	894:901	arg1	microscopy					903:912	laser confocal microscopy	888:912	laser confocal microscopy	888:912	At 7, 14, 42, and 70 days after transplantation, frozen sections were stained with hematoxylin and eosin, followed by DAPI, β-actin, and collagen type-I immunofluorescence staining, and observed using laser confocal microscopy.
26700239	10	25	theme	degradation	1305:1315	arg1	lower					1326:1330	lower	1326:1330	lower	1326:1330	The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples.
26700239	10	25	theme	degradation	1305:1315	arg1	rate					1317:1320	the degradation rate	1301:1320	the degradation rate	1301:1320	The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples.
26700239	2	26	theme	shells	263:268	arg1	application					239:249	the application	235:249	the application of mollusca shells	235:268	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	9	27	theme	absorption	1062:1071	arg1	%					1138:1138	205-496%	1131:1138	205-496%	1131:1138	The water absorption rate of the composite and control group were respectively 205-496% and 417-586%.
26700239	9	27	theme	absorption	1062:1071	arg1	rate					1073:1076	The water absorption rate	1052:1076	The water absorption rate of the composite and control group	1052:1111	The water absorption rate of the composite and control group were respectively 205-496% and 417-586%.
26700239	4	28	theme	Calcium	589:595	arg1	carbonate					597:605	Calcium carbonate	589:605	Calcium carbonate	589:605	Calcium carbonate served as the control.
26700239	4	28	theme	Calcium	589:595	arg1	control					621:627	the control	617:627	the control	617:627	Calcium carbonate served as the control.
26700239	6	29	theme	laser	888:892	arg1	microscopy					903:912	laser confocal microscopy	888:912	laser confocal microscopy	888:912	At 7, 14, 42, and 70 days after transplantation, frozen sections were stained with hematoxylin and eosin, followed by DAPI, β-actin, and collagen type-I immunofluorescence staining, and observed using laser confocal microscopy.
26700239	8	30	theme	mechanical	1029:1038	arg1	properties					1040:1049	significantly different mechanical properties	1005:1049	significantly different mechanical properties	1005:1049	The control and composite samples displayed significantly different mechanical properties.
26700239	2	31	theme	mollusca	254:261	arg1	shells					263:268	mollusca shells	254:268	mollusca shells	254:268	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	1	32	theme	soft	162:165	arg1	therapies					187:195	soft tissue regenerative therapies	162:195	soft tissue regenerative therapies	162:195	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	0	33	theme	shell	34:38	arg1	matrix					40:45	shell matrix	34:45	shell matrix	34:45	A honeycomb composite of mollusca shell matrix and calcium alginate.
26700239	1	34	theme	tissue	167:172	arg1	therapies					187:195	soft tissue regenerative therapies	162:195	soft tissue regenerative therapies	162:195	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	11	35	theme	gel	1379:1381	arg1	mixture					1383:1389	the gel mixture	1375:1389	the gel mixture afforded control over chemical reaction rates	1375:1435	Freezing the gel mixture afforded control over chemical reaction rates.
26700239	2	36	theme	woodiana	368:375	arg1	powder					383:388	Anodonta woodiana shell powder	359:388	Anodonta woodiana shell powder	359:388	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	0	37	theme	mollusca	25:32	arg1	composite					12:20	A honeycomb composite	0:20	A honeycomb composite of mollusca shell matrix and calcium alginate.	0:67	A honeycomb composite of mollusca shell matrix and calcium alginate.
26700239	0	37	theme	mollusca	25:32	arg1	mollusca					25:32	mollusca	25:32	mollusca	25:32	A honeycomb composite of mollusca shell matrix and calcium alginate.
26700239	2	38	theme	varying	424:430	arg1	ratios					437:442	varying mass ratios	424:442	varying mass ratios	424:442	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	10	39	theme	samples	1274:1280	arg1	structure					1257:1265	the subcutaneous structure	1240:1265	the subcutaneous structure of the samples	1240:1280	The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples.
26700239	1	40	theme	regenerative	174:185	arg1	therapies					187:195	soft tissue regenerative therapies	162:195	soft tissue regenerative therapies	162:195	A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies.
26700239	10	41	dep	composite	1158:1166	arg1	ratio					1174:1178	mass ratio	1169:1178	mass ratio of 5:5	1169:1185	The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples.
26700239	9	42	theme	composite	1085:1093	arg1	group					1107:1111	the composite and control group	1081:1111	group	1107:1111	The water absorption rate of the composite and control group were respectively 205-496% and 417-586%.
26700239	2	43	theme	preparing	289:297	arg1	methods					299:305	preparing methods	289:305	preparing methods of calcium alginate	289:325	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	10	44	theme	5:5	1183:1185	arg1	ratio					1174:1178	mass ratio	1169:1178	mass ratio of 5:5	1169:1185	The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples.
26700239	12	45	theme	tissue	1511:1516	arg1	engineering					1518:1528	tissue engineering	1511:1528	tissue engineering	1511:1528	Given these results, the composite is a promising honeycomb scaffold for tissue engineering.
26700239	3	46	theme	alginate	569:576	arg1	composite					578:586	a shell matrix/calcium alginate composite	546:586	a shell matrix/calcium alginate composite	546:586	The mixture was frozen and treated with dilute hydrochloric acid to generate a shell matrix/calcium alginate composite.
26700239	7	47	theme	honeycomb	940:948	arg1	structure					950:958	a honeycomb structure	938:958	a honeycomb structure	938:958	The composite featured a honeycomb structure.
26700239	11	48	theme	reaction	1422:1429	arg1	rates					1431:1435	chemical reaction rates	1413:1435	chemical reaction rates	1413:1435	Freezing the gel mixture afforded control over chemical reaction rates.
26700239	9	49	theme	control	1099:1105	arg1	group					1107:1111	the composite and control group	1081:1111	group	1107:1111	The water absorption rate of the composite and control group were respectively 205-496% and 417-586%.
26700239	0	50	dep	composite	12:20	arg1	matrix					40:45	shell matrix	34:45	shell matrix	34:45	A honeycomb composite of mollusca shell matrix and calcium alginate.
26700239	0	50	dep	composite	12:20	arg1	alginate					59:66	calcium alginate	51:66	calcium alginate	51:66	A honeycomb composite of mollusca shell matrix and calcium alginate.
26700239	9	51	theme	group	1107:1111	arg1	%					1138:1138	205-496%	1131:1138	205-496%	1131:1138	The water absorption rate of the composite and control group were respectively 205-496% and 417-586%.
26700239	9	51	theme	group	1107:1111	arg1	rate					1073:1076	The water absorption rate	1052:1076	The water absorption rate of the composite and control group	1052:1111	The water absorption rate of the composite and control group were respectively 205-496% and 417-586%.
26700239	2	52	theme	alginate	318:325	arg1	methods					299:305	preparing methods	289:305	preparing methods of calcium alginate	289:325	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	2	53	theme	Anodonta	359:366	arg1	powder					383:388	Anodonta woodiana shell powder	359:388	Anodonta woodiana shell powder	359:388	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	3	54	theme	shell	548:552	arg1	composite					578:586	a shell matrix/calcium alginate composite	546:586	a shell matrix/calcium alginate composite	546:586	The mixture was frozen and treated with dilute hydrochloric acid to generate a shell matrix/calcium alginate composite.
26700239	10	55	theme	mass	1169:1172	arg1	ratio					1174:1178	mass ratio	1169:1178	mass ratio of 5:5	1169:1185	The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples.
26700239	12	56	theme	honeycomb	1488:1496	arg1	scaffold					1498:1505	a promising honeycomb scaffold	1476:1505	a promising honeycomb scaffold for tissue engineering	1476:1528	Given these results, the composite is a promising honeycomb scaffold for tissue engineering.
26700239	12	56	theme	honeycomb	1488:1496	arg1	composite					1463:1471	composite	1463:1471	composite	1463:1471	Given these results, the composite is a promising honeycomb scaffold for tissue engineering.
26700239	0	57	theme	calcium	51:57	arg1	alginate					59:66	calcium alginate	51:66	calcium alginate	51:66	A honeycomb composite of mollusca shell matrix and calcium alginate.
26700239	6	58	theme	immunofluorescence	840:857	arg1	staining					859:866	collagen type-I immunofluorescence staining	824:866	collagen type-I immunofluorescence staining	824:866	At 7, 14, 42, and 70 days after transplantation, frozen sections were stained with hematoxylin and eosin, followed by DAPI, β-actin, and collagen type-I immunofluorescence staining, and observed using laser confocal microscopy.
26700239	3	59	theme	matrix/calcium	554:567	arg1	composite					578:586	a shell matrix/calcium alginate composite	546:586	a shell matrix/calcium alginate composite	546:586	The mixture was frozen and treated with dilute hydrochloric acid to generate a shell matrix/calcium alginate composite.
26700239	2	60	theme	sodium	405:410	arg1	alginate					412:419	sodium alginate	405:419	sodium alginate	405:419	To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture.
26700239	10	61	theme	biological	1200:1209	arg1	safety					1211:1216	good biological safety	1195:1216	good biological safety	1195:1216	The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples.
26700239	12	62	theme	promising	1478:1486	arg1	scaffold					1498:1505	a promising honeycomb scaffold	1476:1505	a promising honeycomb scaffold for tissue engineering	1476:1528	Given these results, the composite is a promising honeycomb scaffold for tissue engineering.
26700239	12	62	theme	promising	1478:1486	arg1	composite					1463:1471	composite	1463:1471	composite	1463:1471	Given these results, the composite is a promising honeycomb scaffold for tissue engineering.
28547849	0	0	from	alterations	16:26	arg1	factor-2					85:92	fibroblast growth factor-2	67:92	fibroblast growth factor-2	67:92	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.
28547849	0	0	from	alterations	16:26	arg1	structures					52:61	extracellular matrix structures	31:61	extracellular matrix structures	31:61	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.
28547849	7	1	theme	FGF-2	997:1001	arg1	signaling					1003:1011	FGF-2 signaling	997:1011	FGF-2 signaling	997:1011	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	7	2	theme	kinase	1078:1083	arg1	phosphorylation					1028:1042	the phosphorylation	1024:1042	the phosphorylation of extracellular signal-regulated kinase (Erk1/2)	1024:1092	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	7	3	theme	receptor-HS	952:962	arg1	complex					964:970	the FGF-fibroblast growth factor receptor-HS complex	919:970	the FGF-fibroblast growth factor receptor-HS complex	919:970	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	5	4	dep	modified	749:756	arg1	based					758:762	based	758:762	are modified based on immunohistochemistry	745:786	The heparan sulfate (HS) chains in the fractones of the aged subventricular zone are modified based on immunohistochemistry.
28547849	4	5	theme	growth	548:553	arg1	factor					555:560	store fibroblast growth factor 2	531:562	store fibroblast growth factor 2 (FGF-2)	531:570	We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner.
28547849	4	5	theme	growth	548:553	arg1	FGF-2					565:569	FGF-2	565:569	FGF-2	565:569	We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner.
28547849	1	6	theme	subventricular	171:184	arg1	zone					186:189	the subventricular zone	167:189	the subventricular zone of the lateral ventricle	167:214	Adult neurogenesis in the subventricular zone of the lateral ventricle decreases with age.
28547849	10	7	theme	aged	1567:1570	arg1	zone					1587:1590	the aged subventricular zone	1563:1590	the aged subventricular zone	1563:1590	In conclusion, the data revealed that heparan sulfate 6-O-sulfation is reduced and FGF-2-dependent Erk1/2 signaling is impaired in the aged subventricular zone.
28547849	8	8	theme	phosphorylation	1237:1251	arg1	reduction					1124:1132	a reduction	1122:1132	a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction,	1122:1202	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	8	8	theme	phosphorylation	1237:1251	arg1	failure					1208:1214	failure	1208:1214	failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone	1208:1293	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	4	9	theme	fibroblast	537:546	arg1	factor					555:560	store fibroblast growth factor 2	531:562	store fibroblast growth factor 2 (FGF-2)	531:570	We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner.
28547849	4	9	theme	fibroblast	537:546	arg1	FGF-2					565:569	FGF-2	565:569	FGF-2	565:569	We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner.
28547849	7	10	theme	complex	964:970	arg1	formation					906:914	The formation	902:914	The formation of the FGF-fibroblast growth factor receptor-HS complex	902:970	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	7	10	theme	complex	964:970	arg1	necessary					975:983	necessary	975:983	necessary	975:983	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	11	11	theme	neurogenic	1656:1665	arg1	activity					1667:1674	the reduced neurogenic activity	1644:1674	the reduced neurogenic activity in aging brains	1644:1690	HS modifications in fractones might play a role in the reduced neurogenic activity in aging brains.
28547849	11	12	from	activity	1667:1674	arg1	brains					1685:1690	aging brains	1679:1690	aging brains	1679:1690	HS modifications in fractones might play a role in the reduced neurogenic activity in aging brains.
28547849	11	13	theme	aging	1679:1683	arg1	brains					1685:1690	aging brains	1679:1690	aging brains	1679:1690	HS modifications in fractones might play a role in the reduced neurogenic activity in aging brains.
28547849	1	14	from	neurogenesis	151:162	arg1	zone					186:189	the subventricular zone	167:189	the subventricular zone of the lateral ventricle	167:214	Adult neurogenesis in the subventricular zone of the lateral ventricle decreases with age.
28547849	2	15	theme	contact	333:339	arg1	cells					353:357	fractones, contact neural stem cells	322:357	cells	353:357	In the subventricular zone, the specialized extracellular matrix structures, known as fractones, contact neural stem cells and regulate neurogenesis.
28547849	0	16	theme	aged	122:125	arg1	niche					138:142	the aged neurogenic niche	118:142	the aged neurogenic niche	118:142	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.
28547849	8	17	theme	FGF-2	1177:1181	arg1	transduction					1190:1201	FGF-2 signal transduction	1177:1201	FGF-2 signal transduction	1177:1201	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	8	18	theme	HS	1137:1138	arg1	6-O-sulfation					1140:1152	HS 6-O-sulfation	1137:1152	HS 6-O-sulfation	1137:1152	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	2	19	theme	matrix	294:299	arg1	structures					301:310	the specialized extracellular matrix structures	264:310	the specialized extracellular matrix structures	264:310	In the subventricular zone, the specialized extracellular matrix structures, known as fractones, contact neural stem cells and regulate neurogenesis.
28547849	3	20	theme	heparan	453:459	arg1	proteoglycans					469:481	heparan sulfate proteoglycans	453:481	heparan sulfate proteoglycans	453:481	Fractones are composed of extracellular matrix components, such as heparan sulfate proteoglycans.
28547849	5	21	theme	aged	720:723	arg1	zone					740:743	the aged subventricular zone	716:743	the aged subventricular zone	716:743	The heparan sulfate (HS) chains in the fractones of the aged subventricular zone are modified based on immunohistochemistry.
28547849	9	22	theme	increased	1321:1329	arg1	6-O-endo-sulfatase					1334:1351	increased HS 6-O-endo-sulfatase	1321:1351	increased HS 6-O-endo-sulfatase	1321:1351	In addition, we observed increased HS 6-O-endo-sulfatase, an enzyme that may be responsible for the HS modifications in aged fractones.
28547849	9	22	theme	increased	1321:1329	arg1	enzyme					1357:1362	an enzyme	1354:1362	an enzyme that may be responsible for the HS modifications in aged fractones	1354:1429	In addition, we observed increased HS 6-O-endo-sulfatase, an enzyme that may be responsible for the HS modifications in aged fractones.
28547849	9	22	theme	increased	1321:1329	arg1	responsible					1376:1386	responsible	1376:1386	responsible	1376:1386	In addition, we observed increased HS 6-O-endo-sulfatase, an enzyme that may be responsible for the HS modifications in aged fractones.
28547849	11	23	theme	reduced	1648:1654	arg1	activity					1667:1674	the reduced neurogenic activity	1644:1674	the reduced neurogenic activity in aging brains	1644:1690	HS modifications in fractones might play a role in the reduced neurogenic activity in aging brains.
28547849	6	24	theme	subsequent	841:850	arg1	signaling					858:866	subsequent FGF-2 signaling	841:866	subsequent FGF-2 signaling	841:866	However, how aging affects fractone composition and subsequent FGF-2 signaling and neurogenesis remains unknown.
28547849	2	25	theme	extracellular	280:292	arg1	structures					301:310	the specialized extracellular matrix structures	264:310	the specialized extracellular matrix structures	264:310	In the subventricular zone, the specialized extracellular matrix structures, known as fractones, contact neural stem cells and regulate neurogenesis.
28547849	3	26	theme	sulfate	461:467	arg1	proteoglycans					469:481	heparan sulfate proteoglycans	453:481	heparan sulfate proteoglycans	453:481	Fractones are composed of extracellular matrix components, such as heparan sulfate proteoglycans.
28547849	0	27	theme	sulfate	8:14	arg1	alterations					16:26	Heparan sulfate alterations	0:26	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2	0:92	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.
28547849	8	28	from	failure	1208:1214	arg1	6-O-sulfation					1140:1152	HS 6-O-sulfation	1137:1152	HS 6-O-sulfation	1137:1152	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	8	28	from	failure	1208:1214	arg1	zone					1290:1293	the aged subventricular zone	1266:1293	the aged subventricular zone	1266:1293	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	5	29	theme	subventricular	725:738	arg1	zone					740:743	the aged subventricular zone	716:743	the aged subventricular zone	716:743	The heparan sulfate (HS) chains in the fractones of the aged subventricular zone are modified based on immunohistochemistry.
28547849	9	30	from	modifications	1399:1411	arg1	fractones					1421:1429	aged fractones	1416:1429	aged fractones	1416:1429	In addition, we observed increased HS 6-O-endo-sulfatase, an enzyme that may be responsible for the HS modifications in aged fractones.
28547849	0	31	theme	Heparan	0:6	arg1	sulfate					8:14	Heparan sulfate	0:14	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2	0:92	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.
28547849	7	32	theme	FGF-fibroblast	923:936	arg1	complex					964:970	the FGF-fibroblast growth factor receptor-HS complex	919:970	the FGF-fibroblast growth factor receptor-HS complex	919:970	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	2	33	theme	fractones	322:330	arg1	cells					353:357	fractones, contact neural stem cells	322:357	cells	353:357	In the subventricular zone, the specialized extracellular matrix structures, known as fractones, contact neural stem cells and regulate neurogenesis.
28547849	1	34	theme	lateral	198:204	arg1	ventricle					206:214	the lateral ventricle	194:214	the lateral ventricle	194:214	Adult neurogenesis in the subventricular zone of the lateral ventricle decreases with age.
28547849	10	35	theme	sulfate	1478:1484	arg1	6-O-sulfation					1486:1498	heparan sulfate 6-O-sulfation	1470:1498	heparan sulfate 6-O-sulfation	1470:1498	In conclusion, the data revealed that heparan sulfate 6-O-sulfation is reduced and FGF-2-dependent Erk1/2 signaling is impaired in the aged subventricular zone.
28547849	7	36	theme	growth	938:943	arg1	complex					964:970	the FGF-fibroblast growth factor receptor-HS complex	919:970	the FGF-fibroblast growth factor receptor-HS complex	919:970	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	1	37	theme	ventricle	206:214	arg1	zone					186:189	the subventricular zone	167:189	the subventricular zone of the lateral ventricle	167:214	Adult neurogenesis in the subventricular zone of the lateral ventricle decreases with age.
28547849	4	38	theme	sulfate	584:590	arg1	binding					592:598	heparan sulfate binding	576:598	heparan sulfate binding	576:598	We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner.
28547849	0	39	theme	neurogenic	127:136	arg1	niche					138:142	the aged neurogenic niche	118:142	the aged neurogenic niche	118:142	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.
28547849	9	40	theme	aged	1416:1419	arg1	fractones					1421:1429	aged fractones	1416:1429	aged fractones	1416:1429	In addition, we observed increased HS 6-O-endo-sulfatase, an enzyme that may be responsible for the HS modifications in aged fractones.
28547849	4	41	theme	stem	633:636	arg1	cells					638:642	neural stem cells	626:642	neural stem cells	626:642	We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner.
28547849	0	42	theme	matrix	45:50	arg1	structures					52:61	extracellular matrix structures	31:61	extracellular matrix structures	31:61	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.
28547849	4	43	theme	heparan	576:582	arg1	sulfate					584:590	heparan sulfate	576:590	heparan sulfate binding	576:598	We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner.
28547849	2	44	theme	subventricular	243:256	arg1	zone					258:261	the subventricular zone	239:261	the subventricular zone	239:261	In the subventricular zone, the specialized extracellular matrix structures, known as fractones, contact neural stem cells and regulate neurogenesis.
28547849	8	45	theme	Erk1/2	1256:1261	arg1	phosphorylation					1237:1251	the FGF-2-induced phosphorylation	1219:1251	the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone	1219:1293	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	0	46	theme	extracellular	31:43	arg1	structures					52:61	extracellular matrix structures	31:61	extracellular matrix structures	31:61	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.
28547849	8	47	from	reduction	1124:1132	arg1	6-O-sulfation					1140:1152	HS 6-O-sulfation	1137:1152	HS 6-O-sulfation	1137:1152	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	8	47	from	reduction	1124:1132	arg1	zone					1290:1293	the aged subventricular zone	1266:1293	the aged subventricular zone	1266:1293	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	6	48	theme	FGF-2	852:856	arg1	signaling					858:866	subsequent FGF-2 signaling	841:866	subsequent FGF-2 signaling	841:866	However, how aging affects fractone composition and subsequent FGF-2 signaling and neurogenesis remains unknown.
28547849	9	49	theme	HS	1396:1397	arg1	modifications					1399:1411	the HS modifications	1392:1411	the HS modifications in aged fractones	1392:1429	In addition, we observed increased HS 6-O-endo-sulfatase, an enzyme that may be responsible for the HS modifications in aged fractones.
28547849	8	50	theme	subventricular	1275:1288	arg1	zone					1290:1293	the aged subventricular zone	1266:1293	the aged subventricular zone	1266:1293	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	3	51	theme	extracellular	412:424	arg1	components					433:442	extracellular matrix components	412:442	extracellular matrix components	412:442	Fractones are composed of extracellular matrix components, such as heparan sulfate proteoglycans.
28547849	3	51	theme	extracellular	412:424	arg1	proteoglycans					469:481	heparan sulfate proteoglycans	453:481	heparan sulfate proteoglycans	453:481	Fractones are composed of extracellular matrix components, such as heparan sulfate proteoglycans.
28547849	5	52	theme	heparan	668:674	arg1	HS					685:686	HS	685:686	HS	685:686	The heparan sulfate (HS) chains in the fractones of the aged subventricular zone are modified based on immunohistochemistry.
28547849	5	52	theme	heparan	668:674	arg1	sulfate					676:682	heparan sulfate	668:682	The heparan sulfate (HS) chains in the fractones of the aged subventricular zone	664:743	The heparan sulfate (HS) chains in the fractones of the aged subventricular zone are modified based on immunohistochemistry.
28547849	5	53	theme	zone	740:743	arg1	fractones					703:711	the fractones	699:711	the fractones of the aged subventricular zone	699:743	The heparan sulfate (HS) chains in the fractones of the aged subventricular zone are modified based on immunohistochemistry.
28547849	10	54	theme	FGF-2-dependent	1515:1529	arg1	signaling					1538:1546	FGF-2-dependent Erk1/2 signaling	1515:1546	FGF-2-dependent Erk1/2 signaling	1515:1546	In conclusion, the data revealed that heparan sulfate 6-O-sulfation is reduced and FGF-2-dependent Erk1/2 signaling is impaired in the aged subventricular zone.
28547849	10	55	theme	Erk1/2	1531:1536	arg1	signaling					1538:1546	FGF-2-dependent Erk1/2 signaling	1515:1546	FGF-2-dependent Erk1/2 signaling	1515:1546	In conclusion, the data revealed that heparan sulfate 6-O-sulfation is reduced and FGF-2-dependent Erk1/2 signaling is impaired in the aged subventricular zone.
28547849	3	56	theme	matrix	426:431	arg1	components					433:442	extracellular matrix components	412:442	extracellular matrix components	412:442	Fractones are composed of extracellular matrix components, such as heparan sulfate proteoglycans.
28547849	3	56	theme	matrix	426:431	arg1	proteoglycans					469:481	heparan sulfate proteoglycans	453:481	heparan sulfate proteoglycans	453:481	Fractones are composed of extracellular matrix components, such as heparan sulfate proteoglycans.
28547849	5	57	from	chains	689:694	arg1	fractones					703:711	the fractones	699:711	the fractones of the aged subventricular zone	699:743	The heparan sulfate (HS) chains in the fractones of the aged subventricular zone are modified based on immunohistochemistry.
28547849	5	58	theme	sulfate	676:682	arg1	chains					689:694	The heparan sulfate (HS) chains	664:694	The heparan sulfate (HS) chains in the fractones of the aged subventricular zone	664:743	The heparan sulfate (HS) chains in the fractones of the aged subventricular zone are modified based on immunohistochemistry.
28547849	11	59	theme	HS	1593:1594	arg1	modifications					1596:1608	HS modifications	1593:1608	HS modifications in fractones	1593:1621	HS modifications in fractones might play a role in the reduced neurogenic activity in aging brains.
28547849	2	60	theme	stem	348:351	arg1	cells					353:357	fractones, contact neural stem cells	322:357	cells	353:357	In the subventricular zone, the specialized extracellular matrix structures, known as fractones, contact neural stem cells and regulate neurogenesis.
28547849	10	61	theme	heparan	1470:1476	arg1	sulfate					1478:1484	heparan sulfate	1470:1484	heparan sulfate 6-O-sulfation	1470:1498	In conclusion, the data revealed that heparan sulfate 6-O-sulfation is reduced and FGF-2-dependent Erk1/2 signaling is impaired in the aged subventricular zone.
28547849	7	62	theme	extracellular	1047:1059	arg1	kinase					1078:1083	extracellular signal-regulated kinase	1047:1083	extracellular signal-regulated kinase (Erk1/2)	1047:1092	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	7	62	theme	extracellular	1047:1059	arg1	Erk1/2					1086:1091	Erk1/2	1086:1091	Erk1/2	1086:1091	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	7	63	theme	factor	945:950	arg1	complex					964:970	the FGF-fibroblast growth factor receptor-HS complex	919:970	the FGF-fibroblast growth factor receptor-HS complex	919:970	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	2	64	theme	specialized	268:278	arg1	structures					301:310	the specialized extracellular matrix structures	264:310	the specialized extracellular matrix structures	264:310	In the subventricular zone, the specialized extracellular matrix structures, known as fractones, contact neural stem cells and regulate neurogenesis.
28547849	8	65	from	phosphorylation	1237:1251	arg1	zone					1290:1293	the aged subventricular zone	1266:1293	the aged subventricular zone	1266:1293	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	2	66	theme	neural	341:346	arg1	cells					353:357	fractones, contact neural stem cells	322:357	cells	353:357	In the subventricular zone, the specialized extracellular matrix structures, known as fractones, contact neural stem cells and regulate neurogenesis.
28547849	7	67	theme	signal-regulated	1061:1076	arg1	kinase					1078:1083	extracellular signal-regulated kinase	1047:1083	extracellular signal-regulated kinase (Erk1/2)	1047:1092	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	7	67	theme	signal-regulated	1061:1076	arg1	Erk1/2					1086:1091	Erk1/2	1086:1091	Erk1/2	1086:1091	The formation of the FGF-fibroblast growth factor receptor-HS complex is necessary to activate FGF-2 signaling and induce the phosphorylation of extracellular signal-regulated kinase (Erk1/2).
28547849	6	68	theme	fractone	816:823	arg1	composition					825:835	fractone composition	816:835	fractone composition	816:835	However, how aging affects fractone composition and subsequent FGF-2 signaling and neurogenesis remains unknown.
28547849	8	69	theme	signal	1183:1188	arg1	transduction					1190:1201	FGF-2 signal transduction	1177:1201	FGF-2 signal transduction	1177:1201	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	8	70	from	zone	1290:1293	arg1	reduction					1124:1132	a reduction	1122:1132	a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction,	1122:1202	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	8	70	from	zone	1290:1293	arg1	failure					1208:1214	failure	1208:1214	failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone	1208:1293	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	1	71	theme	Adult	145:149	arg1	neurogenesis					151:162	Adult neurogenesis	145:162	Adult neurogenesis in the subventricular zone of the lateral ventricle	145:214	Adult neurogenesis in the subventricular zone of the lateral ventricle decreases with age.
28547849	8	72	theme	FGF-2-induced	1223:1235	arg1	phosphorylation					1237:1251	the FGF-2-induced phosphorylation	1219:1251	the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone	1219:1293	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	0	73	theme	growth	78:83	arg1	factor-2					85:92	fibroblast growth factor-2	67:92	fibroblast growth factor-2	67:92	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.
28547849	0	74	from	impairment	104:113	arg1	niche					138:142	the aged neurogenic niche	118:142	the aged neurogenic niche	118:142	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.
28547849	8	75	theme	aged	1270:1273	arg1	zone					1290:1293	the aged subventricular zone	1266:1293	the aged subventricular zone	1266:1293	In this study, we observed a reduction in HS 6-O-sulfation, which is critical for FGF-2 signal transduction, and failure of the FGF-2-induced phosphorylation of Erk1/2 in the aged subventricular zone.
28547849	10	76	theme	subventricular	1572:1585	arg1	zone					1587:1590	the aged subventricular zone	1563:1590	the aged subventricular zone	1563:1590	In conclusion, the data revealed that heparan sulfate 6-O-sulfation is reduced and FGF-2-dependent Erk1/2 signaling is impaired in the aged subventricular zone.
28547849	4	77	theme	timely	649:654	arg1	manner					656:661	a timely manner	647:661	a timely manner	647:661	We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner.
28547849	4	78	theme	neural	626:631	arg1	cells					638:642	neural stem cells	626:642	neural stem cells	626:642	We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner.
28547849	0	79	theme	fibroblast	67:76	arg1	factor-2					85:92	fibroblast growth factor-2	67:92	fibroblast growth factor-2	67:92	Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.
28547849	4	80	theme	store	531:535	arg1	factor					555:560	store fibroblast growth factor 2	531:562	store fibroblast growth factor 2 (FGF-2)	531:570	We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner.
28547849	4	80	theme	store	531:535	arg1	FGF-2					565:569	FGF-2	565:569	FGF-2	565:569	We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner.
28547849	9	81	theme	HS	1331:1332	arg1	6-O-endo-sulfatase					1334:1351	increased HS 6-O-endo-sulfatase	1321:1351	increased HS 6-O-endo-sulfatase	1321:1351	In addition, we observed increased HS 6-O-endo-sulfatase, an enzyme that may be responsible for the HS modifications in aged fractones.
28547849	9	81	theme	HS	1331:1332	arg1	enzyme					1357:1362	an enzyme	1354:1362	an enzyme that may be responsible for the HS modifications in aged fractones	1354:1429	In addition, we observed increased HS 6-O-endo-sulfatase, an enzyme that may be responsible for the HS modifications in aged fractones.
28547849	9	81	theme	HS	1331:1332	arg1	responsible					1376:1386	responsible	1376:1386	responsible	1376:1386	In addition, we observed increased HS 6-O-endo-sulfatase, an enzyme that may be responsible for the HS modifications in aged fractones.
28547849	11	82	from	modifications	1596:1608	arg1	fractones					1613:1621	fractones	1613:1621	fractones	1613:1621	HS modifications in fractones might play a role in the reduced neurogenic activity in aging brains.
26117748	3	0	theme	coating	701:707	arg1	surfaces					709:716	coating surfaces	701:716	coating surfaces thereby imparting bioactivity	701:746	The presence of n-ZnO was confirmed to impart antibacterial properties to the coatings against gram-negative bacteria Escherichia coli, while the BG induced the formation of hydroxyapatite on coating surfaces thereby imparting bioactivity, making the coating suitable for bone replacement applications.
26117748	6	1	theme	corrosion	1229:1237	arg1	function					1250:1257	the corrosion protection function	1225:1257	the corrosion protection function of the novel organic/inorganic composite coatings	1225:1307	Polarization curves of the coated substrates made in cell culture media at 37 °C confirmed the corrosion protection function of the novel organic/inorganic composite coatings.
26117748	1	2	theme	organic	190:196	arg1	matrix					198:203	organic matrix	190:203	organic matrix	190:203	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	3	3	dep	Escherichia	627:637	arg1	coli					639:642	coli	639:642	coli	639:642	The presence of n-ZnO was confirmed to impart antibacterial properties to the coatings against gram-negative bacteria Escherichia coli, while the BG induced the formation of hydroxyapatite on coating surfaces thereby imparting bioactivity, making the coating suitable for bone replacement applications.
26117748	0	4	theme	antimicrobial	99:111	arg1	applications					113:124	antimicrobial applications	99:124	antimicrobial applications	99:124	Electrophoretic deposition of ZnO/alginate and ZnO-bioactive glass/alginate composite coatings for antimicrobial applications.
26117748	4	5	theme	thermogravimetric	848:864	arg1	Fourier					881:887	Fourier	881:887	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses	881:1007	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	4	5	theme	thermogravimetric	848:864	arg1	analysis					866:873	thermogravimetric analysis	848:873	thermogravimetric analysis (TG)	848:878	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	4	5	theme	thermogravimetric	848:864	arg1	TG					876:877	TG	876:877	TG	876:877	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	6	6	theme	Polarization	1134:1145	arg1	curves					1147:1152	Polarization curves	1134:1152	Polarization curves of the coated substrates made in cell culture media at 37 °C	1134:1213	Polarization curves of the coated substrates made in cell culture media at 37 °C confirmed the corrosion protection function of the novel organic/inorganic composite coatings.
26117748	3	7	theme	replacement	786:796	arg1	applications					798:809	bone replacement applications	781:809	bone replacement applications	781:809	The presence of n-ZnO was confirmed to impart antibacterial properties to the coatings against gram-negative bacteria Escherichia coli, while the BG induced the formation of hydroxyapatite on coating surfaces thereby imparting bioactivity, making the coating suitable for bone replacement applications.
26117748	3	8	theme	hydroxyapatite	683:696	arg1	formation					670:678	the formation	666:678	the formation of hydroxyapatite	666:696	The presence of n-ZnO was confirmed to impart antibacterial properties to the coatings against gram-negative bacteria Escherichia coli, while the BG induced the formation of hydroxyapatite on coating surfaces thereby imparting bioactivity, making the coating suitable for bone replacement applications.
26117748	2	9	theme	alginate	477:484	arg1	concentration					486:498	a fixed alginate concentration	469:498	a fixed alginate concentration (2 g/L)	469:506	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents were studied for a fixed alginate concentration (2 g/L).
26117748	2	9	theme	alginate	477:484	arg1	g/L					503:505	2 g/L	501:505	2 g/L	501:505	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents were studied for a fixed alginate concentration (2 g/L).
26117748	1	10	theme	electrophoretic	363:377	arg1	EPD					391:393	EPD	391:393	EPD	391:393	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	1	10	theme	electrophoretic	363:377	arg1	deposition					379:388	electrophoretic deposition	363:388	electrophoretic deposition (EPD)	363:394	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	2	11	theme	Different	397:405	arg1	n-ZnO					407:411	Different n-ZnO	397:411	Different n-ZnO (1-10 g/L)	397:422	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents were studied for a fixed alginate concentration (2 g/L).
26117748	2	11	theme	Different	397:405	arg1	g/L					419:421	1-10 g/L	414:421	1-10 g/L	414:421	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents were studied for a fixed alginate concentration (2 g/L).
26117748	2	12	theme	fixed	471:475	arg1	concentration					486:498	a fixed alginate concentration	469:498	a fixed alginate concentration (2 g/L)	469:506	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents were studied for a fixed alginate concentration (2 g/L).
26117748	2	12	theme	fixed	471:475	arg1	g/L					503:505	2 g/L	501:505	2 g/L	501:505	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents were studied for a fixed alginate concentration (2 g/L).
26117748	4	13	theme	dispersive	964:973	arg1	EDS					995:997	EDS	995:997	EDS	995:997	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	4	13	theme	dispersive	964:973	arg1	spectroscopy					981:992	energy dispersive X-ray spectroscopy	957:992	energy dispersive X-ray spectroscopy (EDS)	957:998	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	0	14	theme	Electrophoretic	0:14	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of ZnO/alginate and ZnO-bioactive	0:59	Electrophoretic deposition of ZnO/alginate and ZnO-bioactive glass/alginate composite coatings for antimicrobial applications.
26117748	4	15	dep	spectroscopy	908:919	arg1	analyses					1000:1007	analyses	1000:1007	analyses	1000:1007	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	1	16	theme	zinc	210:213	arg1	n-ZnO					236:240	n-ZnO	236:240	n-ZnO	236:240	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	1	16	theme	zinc	210:213	arg1	nanoparticles					221:233	zinc oxide nanoparticles	210:233	zinc oxide nanoparticles (n-ZnO)	210:241	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	2	17	theme	BG	428:429	arg1	contents					443:450	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents	397:450	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents	397:450	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents were studied for a fixed alginate concentration (2 g/L).
26117748	6	18	theme	culture	1192:1198	arg1	media					1200:1204	cell culture media	1187:1204	cell culture media	1187:1204	Polarization curves of the coated substrates made in cell culture media at 37 °C confirmed the corrosion protection function of the novel organic/inorganic composite coatings.
26117748	3	19	theme	n-ZnO	525:529	arg1	presence					513:520	The presence	509:520	The presence of n-ZnO	509:529	The presence of n-ZnO was confirmed to impart antibacterial properties to the coatings against gram-negative bacteria Escherichia coli, while the BG induced the formation of hydroxyapatite on coating surfaces thereby imparting bioactivity, making the coating suitable for bone replacement applications.
26117748	1	20	theme	oxide	215:219	arg1	n-ZnO					236:240	n-ZnO	236:240	n-ZnO	236:240	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	1	20	theme	oxide	215:219	arg1	nanoparticles					221:233	zinc oxide nanoparticles	210:233	zinc oxide nanoparticles (n-ZnO)	210:241	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	0	21	theme	ZnO/alginate	30:41	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of ZnO/alginate and ZnO-bioactive	0:59	Electrophoretic deposition of ZnO/alginate and ZnO-bioactive glass/alginate composite coatings for antimicrobial applications.
26117748	5	22	theme	coatings	1124:1131	arg1	morphology					1106:1115	the cross section morphology	1088:1115	the cross section morphology of the coatings	1088:1131	Scanning electron microscopy (SEM) was employed to study both the surface and the cross section morphology of the coatings.
26117748	5	22	theme	coatings	1124:1131	arg1	surface					1076:1082	the surface	1072:1082	the surface	1072:1082	Scanning electron microscopy (SEM) was employed to study both the surface and the cross section morphology of the coatings.
26117748	6	23	theme	coatings	1300:1307	arg1	function					1250:1257	the corrosion protection function	1225:1257	the corrosion protection function of the novel organic/inorganic composite coatings	1225:1307	Polarization curves of the coated substrates made in cell culture media at 37 °C confirmed the corrosion protection function of the novel organic/inorganic composite coatings.
26117748	1	24	theme	organic/inorganic	131:147	arg1	coatings					159:166	Two organic/inorganic composite coatings	127:166	Two organic/inorganic composite coatings	127:166	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	1	25	theme	inorganic	285:293	arg1	components					295:304	inorganic components	285:304	inorganic components	285:304	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	6	26	theme	composite	1290:1298	arg1	coatings					1300:1307	the novel organic/inorganic composite coatings	1262:1307	the novel organic/inorganic composite coatings	1262:1307	Polarization curves of the coated substrates made in cell culture media at 37 °C confirmed the corrosion protection function of the novel organic/inorganic composite coatings.
26117748	3	27	theme	gram-negative	604:616	arg1	bacteria					618:625	gram-negative bacteria	604:625	gram-negative bacteria Escherichia coli	604:642	The presence of n-ZnO was confirmed to impart antibacterial properties to the coatings against gram-negative bacteria Escherichia coli, while the BG induced the formation of hydroxyapatite on coating surfaces thereby imparting bioactivity, making the coating suitable for bone replacement applications.
26117748	1	28	theme	composite	149:157	arg1	coatings					159:166	Two organic/inorganic composite coatings	127:166	Two organic/inorganic composite coatings	127:166	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	0	29	theme	ZnO-bioactive	47:59	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of ZnO/alginate and ZnO-bioactive	0:59	Electrophoretic deposition of ZnO/alginate and ZnO-bioactive glass/alginate composite coatings for antimicrobial applications.
26117748	6	30	theme	organic/inorganic	1272:1288	arg1	coatings					1300:1307	the novel organic/inorganic composite coatings	1262:1307	the novel organic/inorganic composite coatings	1262:1307	Polarization curves of the coated substrates made in cell culture media at 37 °C confirmed the corrosion protection function of the novel organic/inorganic composite coatings.
26117748	6	31	theme	cell	1187:1190	arg1	media					1200:1204	cell culture media	1187:1204	cell culture media	1187:1204	Polarization curves of the coated substrates made in cell culture media at 37 °C confirmed the corrosion protection function of the novel organic/inorganic composite coatings.
26117748	4	32	theme	energy	957:962	arg1	EDS					995:997	EDS	995:997	EDS	995:997	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	4	32	theme	energy	957:962	arg1	spectroscopy					981:992	energy dispersive X-ray spectroscopy	957:992	energy dispersive X-ray spectroscopy (EDS)	957:998	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	5	33	theme	cross	1092:1096	arg1	morphology					1106:1115	the cross section morphology	1088:1115	the cross section morphology of the coatings	1088:1131	Scanning electron microscopy (SEM) was employed to study both the surface and the cross section morphology of the coatings.
26117748	6	34	theme	novel	1266:1270	arg1	coatings					1300:1307	the novel organic/inorganic composite coatings	1262:1307	the novel organic/inorganic composite coatings	1262:1307	Polarization curves of the coated substrates made in cell culture media at 37 °C confirmed the corrosion protection function of the novel organic/inorganic composite coatings.
26117748	2	35	theme	n-ZnO	407:411	arg1	contents					443:450	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents	397:450	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents	397:450	Different n-ZnO (1-10 g/L) and BG (1-1.5 g/L) contents were studied for a fixed alginate concentration (2 g/L).
26117748	0	36	theme	composite	76:84	arg1	coatings					86:93	composite coatings	76:93	composite coatings for antimicrobial applications	76:124	Electrophoretic deposition of ZnO/alginate and ZnO-bioactive glass/alginate composite coatings for antimicrobial applications.
26117748	5	37	theme	section	1098:1104	arg1	morphology					1106:1115	the cross section morphology	1088:1115	the cross section morphology of the coatings	1088:1131	Scanning electron microscopy (SEM) was employed to study both the surface and the cross section morphology of the coatings.
26117748	4	38	theme	Coating	812:818	arg1	composition					820:830	Coating composition	812:830	Coating composition	812:830	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	4	39	theme	X-ray	975:979	arg1	EDS					995:997	EDS	995:997	EDS	995:997	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	4	39	theme	X-ray	975:979	arg1	spectroscopy					981:992	energy dispersive X-ray spectroscopy	957:992	energy dispersive X-ray spectroscopy (EDS)	957:998	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	6	40	theme	substrates	1168:1177	arg1	curves					1147:1152	Polarization curves	1134:1152	Polarization curves of the coated substrates made in cell culture media at 37 °C	1134:1213	Polarization curves of the coated substrates made in cell culture media at 37 °C confirmed the corrosion protection function of the novel organic/inorganic composite coatings.
26117748	1	41	theme	biomedical	320:329	arg1	applications					331:342	biomedical applications	320:342	biomedical applications	320:342	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	5	42	theme	Scanning	1010:1017	arg1	SEM					1040:1042	SEM	1040:1042	SEM	1040:1042	Scanning electron microscopy (SEM) was employed to study both the surface and the cross section morphology of the coatings.
26117748	5	42	theme	Scanning	1010:1017	arg1	microscopy					1028:1037	Scanning electron microscopy	1010:1037	Scanning electron microscopy (SEM)	1010:1043	Scanning electron microscopy (SEM) was employed to study both the surface and the cross section morphology of the coatings.
26117748	6	43	theme	coated	1161:1166	arg1	substrates					1168:1177	the coated substrates	1157:1177	the coated substrates made in cell culture media at 37 °C	1157:1213	Polarization curves of the coated substrates made in cell culture media at 37 °C confirmed the corrosion protection function of the novel organic/inorganic composite coatings.
26117748	6	44	theme	protection	1239:1248	arg1	function					1250:1257	the corrosion protection function	1225:1257	the corrosion protection function of the novel organic/inorganic composite coatings	1225:1307	Polarization curves of the coated substrates made in cell culture media at 37 °C confirmed the corrosion protection function of the novel organic/inorganic composite coatings.
26117748	3	45	theme	Escherichia	627:637	arg1	bacteria					618:625	gram-negative bacteria	604:625	gram-negative bacteria Escherichia coli	604:642	The presence of n-ZnO was confirmed to impart antibacterial properties to the coatings against gram-negative bacteria Escherichia coli, while the BG induced the formation of hydroxyapatite on coating surfaces thereby imparting bioactivity, making the coating suitable for bone replacement applications.
26117748	5	46	theme	electron	1019:1026	arg1	SEM					1040:1042	SEM	1040:1042	SEM	1040:1042	Scanning electron microscopy (SEM) was employed to study both the surface and the cross section morphology of the coatings.
26117748	5	46	theme	electron	1019:1026	arg1	microscopy					1028:1037	Scanning electron microscopy	1010:1037	Scanning electron microscopy (SEM)	1010:1043	Scanning electron microscopy (SEM) was employed to study both the surface and the cross section morphology of the coatings.
26117748	3	47	theme	bone	781:784	arg1	applications					798:809	bone replacement applications	781:809	bone replacement applications	781:809	The presence of n-ZnO was confirmed to impart antibacterial properties to the coatings against gram-negative bacteria Escherichia coli, while the BG induced the formation of hydroxyapatite on coating surfaces thereby imparting bioactivity, making the coating suitable for bone replacement applications.
26117748	4	48	dep	transform	889:897	arg1	infrared					899:906	infrared	899:906	transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses	889:1007	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	3	49	theme	antibacterial	555:567	arg1	properties					569:578	antibacterial properties	555:578	antibacterial properties	555:578	The presence of n-ZnO was confirmed to impart antibacterial properties to the coatings against gram-negative bacteria Escherichia coli, while the BG induced the formation of hydroxyapatite on coating surfaces thereby imparting bioactivity, making the coating suitable for bone replacement applications.
26117748	4	50	dep	Fourier	881:887	arg1	transform					889:897	transform	889:897	transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses	889:1007	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	4	51	theme	X-ray	929:933	arg1	XRD					948:950	XRD	948:950	XRD	948:950	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	4	51	theme	X-ray	929:933	arg1	diffraction					935:945	X-ray diffraction	929:945	X-ray diffraction (XRD)	929:951	Coating composition was analyzed by thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analyses.
26117748	1	52	theme	bioactive	260:268	arg1	BG					277:278	BG	277:278	BG	277:278	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
26117748	1	52	theme	bioactive	260:268	arg1	glass					270:274	bioactive glass	260:274	bioactive glass (BG)	260:279	Two organic/inorganic composite coatings based on alginate, as organic matrix, and zinc oxide nanoparticles (n-ZnO) with and without bioactive glass (BG), as inorganic components, intended for biomedical applications, were developed by electrophoretic deposition (EPD).
27059718	2	0	theme	host	481:484	arg1	metabolism					486:495	host metabolism	481:495	host metabolism	481:495	Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota-dependent effects of oligofructose on body composition and host metabolism.
27059718	5	1	theme	ampicillin	1086:1095	arg1	combination					1071:1081	the combination	1067:1081	the combination of ampicillin and neomycin	1067:1108	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	6	2	dep	species	1342:1348	arg1	modified					1485:1492	modified	1485:1492	is modified by antibiotics in obese rats	1482:1521	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	3	3	theme	Diet-induced	498:509	arg1	rats					537:540	Diet-induced obese male Sprague Dawley rats	498:540	Diet-induced obese male Sprague Dawley rats	498:540	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	1	4	theme	gut	237:239	arg1	microbiota					241:250	gut microbiota	237:250	gut microbiota	237:250	Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health.
27059718	0	5	theme	obese	74:78	arg1	rats					80:83	obese rats	74:83	obese rats	74:83	Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	3	6	dep	ampicillin	688:697	arg1	3					685:685	3	685:685	3	685:685	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	3	7	theme	obese	511:515	arg1	rats					537:540	Diet-induced obese male Sprague Dawley rats	498:540	Diet-induced obese male Sprague Dawley rats	498:540	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	6	8	theme	B.	1406:1407	arg1	Improvement					1438:1448	M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement	1374:1448	M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose	1374:1480	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	2	9	from	effects	432:438	arg1	metabolism					486:495	host metabolism	481:495	host metabolism	481:495	Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota-dependent effects of oligofructose on body composition and host metabolism.
27059718	2	9	from	effects	432:438	arg1	composition					465:475	body composition	460:475	body composition	460:475	Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota-dependent effects of oligofructose on body composition and host metabolism.
27059718	3	10	theme	10	787:788	arg1	%					789:789	%	789:789	%	789:789	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	6	11	with	Improvement	1438:1448	arg1	oligofructose					1468:1480	oligofructose	1468:1480	oligofructose	1468:1480	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	3	12	dep	control	654:660	arg1	1					639:639	1	639:639	1	639:639	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	6	13	from	Improvement	1438:1448	arg1	adiposity					1453:1461	adiposity	1453:1461	adiposity	1453:1461	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	4	14	from	decrease	868:875	arg1	adiposity					880:888	adiposity	880:888	adiposity	880:888	Combining oligofructose with ampicillin treatment blunted the decrease in adiposity seen with oligofructose.
27059718	6	15	theme	species	1342:1348	arg1	presence					1311:1318	the presence	1307:1318	the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats	1307:1521	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	6	16	theme	metabolic	1256:1264	arg1	health					1266:1271	metabolic health	1256:1271	metabolic health	1256:1271	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	3	17	dep	ampicillin/neomycin	765:783	arg1	6					762:762	6	762:762	6	762:762	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	3	18	theme	Dawley	530:535	arg1	rats					537:540	Diet-induced obese male Sprague Dawley rats	498:540	Diet-induced obese male Sprague Dawley rats	498:540	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	1	19	theme	growing	163:169	arg1	interest					171:178	growing interest	163:178	growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health	163:285	Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health.
27059718	2	20	theme	oligofructose	443:455	arg1	effects					432:438	the gut microbiota-dependent effects	403:438	the gut microbiota-dependent effects of oligofructose on body composition and host metabolism	403:495	Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota-dependent effects of oligofructose on body composition and host metabolism.
27059718	3	21	theme	10	666:667	arg1	%					668:668	%	668:668	%	668:668	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	5	22	theme	oligofructose-induced	1158:1178	arg1	decrease					1180:1187	the oligofructose-induced decrease	1154:1187	the oligofructose-induced decrease in adiposity	1154:1200	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	2	23	theme	fat	381:383	arg1	mass					385:388	excess fat mass	374:388	excess fat mass	374:388	Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota-dependent effects of oligofructose on body composition and host metabolism.
27059718	3	24	theme	%	668:668	arg1	oligofructose					670:682	10% oligofructose	666:682	2) 10% oligofructose	663:682	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	0	25	mod	modified	47:54	arg3	antibiotics					59:69	antibiotics	59:69	antibiotics in obese rats	59:83	Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	0	25	mod	modified	47:54	arg1	Improvement					0:10	Improvement	0:10	Improvement in adiposity with oligofructose	0:42	Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	3	26	theme	high-fat/sucrose	560:575	arg1	diet					577:580	a background high-fat/sucrose diet	547:580	a background high-fat/sucrose diet	547:580	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	2	27	theme	excess	374:379	arg1	mass					385:388	excess fat mass	374:388	excess fat mass	374:388	Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota-dependent effects of oligofructose on body composition and host metabolism.
27059718	1	28	theme	human	264:268	arg1	health					280:285	human metabolic health	264:285	human metabolic health	264:285	Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health.
27059718	6	29	theme	host	1237:1240	arg1	adiposity					1242:1250	host adiposity	1237:1250	host adiposity	1237:1250	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	2	30	theme	microbiota-dependent	411:430	arg1	effects					432:438	the gut microbiota-dependent effects	403:438	the gut microbiota-dependent effects of oligofructose on body composition and host metabolism	403:495	Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota-dependent effects of oligofructose on body composition and host metabolism.
27059718	1	31	theme	metabolic	270:278	arg1	health					280:285	human metabolic health	264:285	human metabolic health	264:285	Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health.
27059718	6	32	theme	specific	1323:1330	arg1	species					1342:1348	specific microbial species	1323:1348	specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats	1323:1521	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	6	33	mod	modified	1485:1492	arg3	antibiotics					1497:1507	antibiotics	1497:1507	antibiotics in obese rats	1497:1521	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	6	33	mod	modified	1485:1492	arg1	Improvement					1438:1448	M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement	1374:1448	M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose	1374:1480	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	2	34	theme	gut	407:409	arg1	effects					432:438	the gut microbiota-dependent effects	403:438	the gut microbiota-dependent effects of oligofructose on body composition and host metabolism	403:495	Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota-dependent effects of oligofructose on body composition and host metabolism.
27059718	4	35	theme	ampicillin	835:844	arg1	treatment					846:854	ampicillin treatment	835:854	ampicillin treatment	835:854	Combining oligofructose with ampicillin treatment blunted the decrease in adiposity seen with oligofructose.
27059718	1	36	theme	intimate	96:103	arg1	link					105:108	the intimate link	92:108	the intimate link between gut microbiota and host physiology	92:151	Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health.
27059718	5	37	theme	total	950:954	arg1	bacteria					956:963	total bacteria	950:963	total bacteria	950:963	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	5	38	from	bacteria	956:963	arg1	contrast					1057:1064	contrast	1057:1064	contrast	1057:1064	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	5	38	from	bacteria	956:963	arg1	Bifidobacterium					1020:1034	Bifidobacterium	1020:1034	Bifidobacterium	1020:1034	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	2	39	theme	body	460:463	arg1	composition					465:475	body composition	460:475	body composition	460:475	Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota-dependent effects of oligofructose on body composition and host metabolism.
27059718	6	40	theme	obese	1512:1516	arg1	rats					1518:1521	obese rats	1512:1521	obese rats	1512:1521	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	5	41	from	decrease	1180:1187	arg1	adiposity					1192:1200	adiposity	1192:1200	adiposity	1192:1200	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	3	42	theme	diets	623:627	arg1	diets					623:627	the following diets	609:627	the following diets	609:627	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	3	42	theme	diets	623:627	arg1	one					602:604	one	602:604	one	602:604	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	6	43	dep	B.	1406:1407	arg1	H.					1387:1388	H.	1387:1388	H.	1387:1388	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	6	43	dep	B.	1406:1407	arg1	Paul					1381:1384	M. R., Paul, H. A., Geuking, M. B.	1374:1407	Paul	1381:1384	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	6	44	theme	microbial	1332:1340	arg1	species					1342:1348	specific microbial species	1323:1348	specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats	1323:1521	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	5	45	theme	total	1118:1122	arg1	bacteria					1124:1131	total bacteria	1118:1131	total bacteria	1118:1131	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	0	46	from	antibiotics	59:69	arg1	rats					80:83	obese rats	74:83	obese rats	74:83	Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	6	47	theme	Oligofructose-mediated	1203:1224	arg1	effects					1226:1232	Oligofructose-mediated effects	1203:1232	Oligofructose-mediated effects on host adiposity and metabolic health	1203:1271	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	3	48	dep	ampicillin	703:712	arg1	4					700:700	4	700:700	4	700:700	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	0	49	from	Improvement	0:10	arg1	adiposity					15:23	adiposity	15:23	adiposity	15:23	Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	3	50	dep	oligofructose	670:682	arg1	2					663:663	2	663:663	2	663:663	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	6	51	theme	dependent	1294:1302	arg1	part					1289:1292	part	1289:1292	part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats	1289:1521	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	5	52	theme	impeded	977:983	arg1	increases					1007:1015	ampicillin impeded oligofructose-induced increases	966:1015	ampicillin impeded oligofructose-induced increases in Bifidobacterium	966:1034	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	5	53	theme	oligofructose-induced	985:1005	arg1	increases					1007:1015	ampicillin impeded oligofructose-induced increases	966:1015	ampicillin impeded oligofructose-induced increases in Bifidobacterium	966:1034	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	6	54	from	antibiotics	1497:1507	arg1	rats					1518:1521	obese rats	1512:1521	obese rats	1512:1521	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	3	55	theme	high-energy	642:652	arg1	control					654:660	1) high-energy control	639:660	1) high-energy control	639:660	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	0	56	with	Improvement	0:10	arg1	oligofructose					30:42	oligofructose	30:42	oligofructose	30:42	Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	1	57	theme	gut	118:120	arg1	microbiota					122:131	gut microbiota	118:131	gut microbiota	118:131	Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health.
27059718	3	58	dep	ampicillin/neomycin	738:756	arg1	5					735:735	5	735:735	5	735:735	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	3	59	theme	following	613:621	arg1	diets					623:627	the following diets	609:627	the following diets	609:627	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	2	60	theme	prebiotic	314:322	arg1	oligofructose					324:336	the prebiotic oligofructose	310:336	the prebiotic oligofructose	310:336	Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota-dependent effects of oligofructose on body composition and host metabolism.
27059718	3	61	theme	background	549:558	arg1	diet					577:580	a background high-fat/sucrose diet	547:580	a background high-fat/sucrose diet	547:580	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	5	62	from	increases	1007:1015	arg1	contrast					1057:1064	contrast	1057:1064	contrast	1057:1064	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	5	62	from	increases	1007:1015	arg1	Bifidobacterium					1020:1034	Bifidobacterium	1020:1034	Bifidobacterium	1020:1034	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	5	63	theme	neomycin	1101:1108	arg1	combination					1071:1081	the combination	1067:1081	the combination of ampicillin and neomycin	1067:1108	Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose-induced increases in Bifidobacterium and Lactobacillus In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose-induced decrease in adiposity.
27059718	3	64	theme	male	517:520	arg1	rats					537:540	Diet-induced obese male Sprague Dawley rats	498:540	Diet-induced obese male Sprague Dawley rats	498:540	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	6	65	from	effects	1226:1232	arg1	adiposity					1242:1250	host adiposity	1237:1250	host adiposity	1237:1250	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	6	65	from	effects	1226:1232	arg1	health					1266:1271	metabolic health	1256:1271	metabolic health	1256:1271	Oligofructose-mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.-Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats.
27059718	3	66	theme	10	716:717	arg1	%					718:718	%	718:718	%	718:718	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	1	67	theme	host	137:140	arg1	physiology					142:151	host physiology	137:151	host physiology	137:151	Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health.
27059718	3	68	theme	%	789:789	arg1	oligofructose					791:803	10% oligofructose	787:803	10% oligofructose	787:803	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
27059718	3	69	theme	%	718:718	arg1	oligofructose					720:732	10% oligofructose	716:732	10% oligofructose	716:732	Diet-induced obese male Sprague Dawley rats, fed a background high-fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high-energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose.
24115568	7	0	theme	drug-loaded	1175:1185	arg1	CS-MSNs					1187:1193	the drug-loaded CS-MSNs	1171:1193	the drug-loaded CS-MSNs	1171:1193	In addition, the in vitro kill potency against MCF-7 breast-cancer cells was enhanced over time, as well as with increasing concentration of the drug-loaded CS-MSNs.
24115568	6	1	theme	perfect	906:912	arg1	biocompatibility					914:929	the perfect biocompatibility	902:929	the perfect biocompatibility of chitosan	902:941	Moreover, thanks to the perfect biocompatibility of chitosan, the CS-MSNs exhibited lower cytotoxicity than that of the naked MSNs in an MTT assay.
24115568	6	2	from	MSNs	1008:1011	arg1	assay					1023:1027	an MTT assay	1016:1027	an MTT assay	1016:1027	Moreover, thanks to the perfect biocompatibility of chitosan, the CS-MSNs exhibited lower cytotoxicity than that of the naked MSNs in an MTT assay.
24115568	1	3	theme	mesoporous	187:196	arg1	MSNs					220:223	MSNs	220:223	MSNs	220:223	Herein, we present a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs).
24115568	1	3	theme	mesoporous	187:196	arg1	nanoparticles					205:217	pH-responsive chitosan-capped mesoporous silica nanoparticles	157:217	pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs)	157:224	Herein, we present a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs).
24115568	7	4	theme	CS-MSNs	1187:1193	arg1	concentration					1154:1166	concentration	1154:1166	concentration of the drug-loaded CS-MSNs	1154:1193	In addition, the in vitro kill potency against MCF-7 breast-cancer cells was enhanced over time, as well as with increasing concentration of the drug-loaded CS-MSNs.
24115568	1	5	theme	silica	198:203	arg1	MSNs					220:223	MSNs	220:223	MSNs	220:223	Herein, we present a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs).
24115568	1	5	theme	silica	198:203	arg1	nanoparticles					205:217	pH-responsive chitosan-capped mesoporous silica nanoparticles	157:217	pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs)	157:224	Herein, we present a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs).
24115568	8	6	theme	cancer	1292:1297	arg1	therapy					1299:1305	cancer therapy	1292:1305	cancer therapy	1292:1305	These results indicate that CS-MSNs are promising candidates for pH-responsive drug delivery in cancer therapy.
24115568	7	7	theme	MCF-7	1077:1081	arg1	cells					1097:1101	MCF-7 breast-cancer cells	1077:1101	MCF-7 breast-cancer cells	1077:1101	In addition, the in vitro kill potency against MCF-7 breast-cancer cells was enhanced over time, as well as with increasing concentration of the drug-loaded CS-MSNs.
24115568	8	8	from	delivery	1280:1287	arg1	therapy					1299:1305	cancer therapy	1292:1305	cancer therapy	1292:1305	These results indicate that CS-MSNs are promising candidates for pH-responsive drug delivery in cancer therapy.
24115568	4	9	theme	environmental	764:776	arg1	response					778:785	excellent environmental response	754:785	excellent environmental response	754:785	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	3	10	theme	MSNs	400:403	arg1	surface					385:391	the surface	381:391	the surface of the MSNs, which served as a bridge to link between MSNs and chitosan, which is ubiquitous in nature and commercially available	381:521	Subsequently, (3-glycidyloxypropyl)trimethoxysilane was grafted onto the surface of the MSNs, which served as a bridge to link between MSNs and chitosan, which is ubiquitous in nature and commercially available.
24115568	7	11	theme	breast-cancer	1083:1095	arg1	cells					1097:1101	MCF-7 breast-cancer cells	1077:1101	MCF-7 breast-cancer cells	1077:1101	In addition, the in vitro kill potency against MCF-7 breast-cancer cells was enhanced over time, as well as with increasing concentration of the drug-loaded CS-MSNs.
24115568	6	12	theme	chitosan	934:941	arg1	biocompatibility					914:929	the perfect biocompatibility	902:929	the perfect biocompatibility of chitosan	902:941	Moreover, thanks to the perfect biocompatibility of chitosan, the CS-MSNs exhibited lower cytotoxicity than that of the naked MSNs in an MTT assay.
24115568	4	13	theme	excellent	754:762	arg1	response					778:785	excellent environmental response	754:785	excellent environmental response	754:785	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	8	14	theme	drug	1275:1278	arg1	delivery					1280:1287	pH-responsive drug delivery	1261:1287	pH-responsive drug delivery in cancer therapy	1261:1305	These results indicate that CS-MSNs are promising candidates for pH-responsive drug delivery in cancer therapy.
24115568	8	15	theme	pH-responsive	1261:1273	arg1	delivery					1280:1287	pH-responsive drug delivery	1261:1287	pH-responsive drug delivery in cancer therapy	1261:1305	These results indicate that CS-MSNs are promising candidates for pH-responsive drug delivery in cancer therapy.
24115568	1	16	theme	nanoparticles	205:217	arg1	synthesis					144:152	a straightforward synthesis	126:152	a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs)	126:224	Herein, we present a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs).
24115568	5	17	theme	release	847:853	arg1	degree					832:837	the degree	828:837	the degree of drug release	828:853	As the pH value of the media decreased, the degree of drug release correspondingly increased.
24115568	0	18	theme	mesoporous	16:25	arg1	silica					27:32	Chitosan-capped mesoporous silica	0:32	Chitosan-capped mesoporous silica	0:32	Chitosan-capped mesoporous silica nanoparticles as pH-responsive nanocarriers for controlled drug release.
24115568	7	19	dep	in	1047:1048	arg1	vitro					1050:1054	vitro	1050:1054	vitro	1050:1054	In addition, the in vitro kill potency against MCF-7 breast-cancer cells was enhanced over time, as well as with increasing concentration of the drug-loaded CS-MSNs.
24115568	7	19	dep	in	1047:1048	arg1	kill					1056:1059	kill	1056:1059	kill	1056:1059	In addition, the in vitro kill potency against MCF-7 breast-cancer cells was enhanced over time, as well as with increasing concentration of the drug-loaded CS-MSNs.
24115568	3	20	from	nature	489:494	arg1	bridge					424:429	a bridge	422:429	a bridge	422:429	Subsequently, (3-glycidyloxypropyl)trimethoxysilane was grafted onto the surface of the MSNs, which served as a bridge to link between MSNs and chitosan, which is ubiquitous in nature and commercially available.
24115568	3	20	from	nature	489:494	arg1	MSNs					400:403	the MSNs	396:403	the MSNs	396:403	Subsequently, (3-glycidyloxypropyl)trimethoxysilane was grafted onto the surface of the MSNs, which served as a bridge to link between MSNs and chitosan, which is ubiquitous in nature and commercially available.
24115568	3	20	from	nature	489:494	arg1	ubiquitous					475:484	ubiquitous	475:484	ubiquitous	475:484	Subsequently, (3-glycidyloxypropyl)trimethoxysilane was grafted onto the surface of the MSNs, which served as a bridge to link between MSNs and chitosan, which is ubiquitous in nature and commercially available.
24115568	0	21	theme	Chitosan-capped	0:14	arg1	silica					27:32	Chitosan-capped mesoporous silica	0:32	Chitosan-capped mesoporous silica	0:32	Chitosan-capped mesoporous silica nanoparticles as pH-responsive nanocarriers for controlled drug release.
24115568	5	22	theme	media	811:815	arg1	value					798:802	the pH value	791:802	the pH value of the media	791:815	As the pH value of the media decreased, the degree of drug release correspondingly increased.
24115568	6	23	theme	lower	966:970	arg1	cytotoxicity					972:983	lower cytotoxicity	966:983	lower cytotoxicity	966:983	Moreover, thanks to the perfect biocompatibility of chitosan, the CS-MSNs exhibited lower cytotoxicity than that of the naked MSNs in an MTT assay.
24115568	4	24	theme	doxorubicin	639:649	arg1	hydrochloride					651:663	doxorubicin hydrochloride	639:663	doxorubicin hydrochloride	639:663	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	4	24	theme	doxorubicin	639:649	arg1	drug					633:636	an anti-cancer drug	618:636	an anti-cancer drug	618:636	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	7	25	theme	in	1047:1048	arg1	potency					1061:1067	the in vitro kill potency	1043:1067	the in vitro kill potency against MCF-7 breast-cancer cells	1043:1101	In addition, the in vitro kill potency against MCF-7 breast-cancer cells was enhanced over time, as well as with increasing concentration of the drug-loaded CS-MSNs.
24115568	2	26	used	used	259:262	arg2	MSNs					245:248	These MCM-41-type MSNs	227:248	These MCM-41-type MSNs	227:248	These MCM-41-type MSNs could be used as nanocapsules to accommodate guest molecules.
24115568	2	26	used	used	259:262	arg2	nanocapsules					267:278	nanocapsules	267:278	nanocapsules	267:278	These MCM-41-type MSNs could be used as nanocapsules to accommodate guest molecules.
24115568	0	27	theme	pH-responsive	51:63	arg1	nanocarriers					65:76	pH-responsive nanocarriers	51:76	pH-responsive nanocarriers for controlled drug release	51:104	Chitosan-capped mesoporous silica nanoparticles as pH-responsive nanocarriers for controlled drug release.
24115568	4	28	theme	chitosan	581:588	arg1	features					569:576	the pH-responsive and biocompatible features	533:576	the pH-responsive and biocompatible features of chitosan	533:588	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	5	29	theme	drug	842:845	arg1	release					847:853	drug release	842:853	drug release	842:853	As the pH value of the media decreased, the degree of drug release correspondingly increased.
24115568	4	30	theme	pH-responsive	537:549	arg1	features					569:576	the pH-responsive and biocompatible features	533:576	the pH-responsive and biocompatible features of chitosan	533:588	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	4	31	dep	loading	595:601	arg1	the					591:593	the	591:593	the	591:593	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	6	32	theme	MTT	1019:1021	arg1	assay					1023:1027	an MTT assay	1016:1027	an MTT assay	1016:1027	Moreover, thanks to the perfect biocompatibility of chitosan, the CS-MSNs exhibited lower cytotoxicity than that of the naked MSNs in an MTT assay.
24115568	5	33	theme	pH	795:796	arg1	value					798:802	the pH value	791:802	the pH value of the media	791:815	As the pH value of the media decreased, the degree of drug release correspondingly increased.
24115568	4	34	theme	composite	706:714	arg1	CS-MSNs					738:744	CS-MSNs	738:744	CS-MSNs	738:744	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	4	34	theme	composite	706:714	arg1	MSNs					732:735	the composite chitosan-capped MSNs	702:735	the composite chitosan-capped MSNs (CS-MSNs)	702:745	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	4	35	theme	biocompatible	555:567	arg1	features					569:576	the pH-responsive and biocompatible features	533:576	the pH-responsive and biocompatible features of chitosan	533:588	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	1	36	theme	straightforward	128:142	arg1	synthesis					144:152	a straightforward synthesis	126:152	a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs)	126:224	Herein, we present a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs).
24115568	2	37	theme	MCM-41-type	233:243	arg1	nanocapsules					267:278	nanocapsules	267:278	nanocapsules	267:278	These MCM-41-type MSNs could be used as nanocapsules to accommodate guest molecules.
24115568	2	37	theme	MCM-41-type	233:243	arg1	MSNs					245:248	These MCM-41-type MSNs	227:248	These MCM-41-type MSNs	227:248	These MCM-41-type MSNs could be used as nanocapsules to accommodate guest molecules.
24115568	2	38	theme	guest	295:299	arg1	molecules					301:309	guest molecules	295:309	guest molecules	295:309	These MCM-41-type MSNs could be used as nanocapsules to accommodate guest molecules.
24115568	3	39	from	ubiquitous	475:484	arg1	nature					489:494	nature	489:494	nature	489:494	Subsequently, (3-glycidyloxypropyl)trimethoxysilane was grafted onto the surface of the MSNs, which served as a bridge to link between MSNs and chitosan, which is ubiquitous in nature and commercially available.
24115568	3	40	theme	3-glycidyloxypropyl	327:345	arg1	trimethoxysilane					347:362	(3-glycidyloxypropyl)trimethoxysilane	326:362	(3-glycidyloxypropyl)trimethoxysilane	326:362	Subsequently, (3-glycidyloxypropyl)trimethoxysilane was grafted onto the surface of the MSNs, which served as a bridge to link between MSNs and chitosan, which is ubiquitous in nature and commercially available.
24115568	4	41	theme	chitosan-capped	716:730	arg1	CS-MSNs					738:744	CS-MSNs	738:744	CS-MSNs	738:744	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	4	41	theme	chitosan-capped	716:730	arg1	MSNs					732:735	the composite chitosan-capped MSNs	702:735	the composite chitosan-capped MSNs (CS-MSNs)	702:745	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	0	42	theme	drug	93:96	arg1	release					98:104	controlled drug release	82:104	controlled drug release	82:104	Chitosan-capped mesoporous silica nanoparticles as pH-responsive nanocarriers for controlled drug release.
24115568	4	43	dep	carried	671:677	arg1	showed					747:752	showed	747:752	showed excellent environmental response	747:785	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	4	43	dep	carried	671:677	arg1	out					679:681	out	679:681	out	679:681	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	8	44	theme	promising	1236:1244	arg1	CS-MSNs					1224:1230	CS-MSNs	1224:1230	CS-MSNs	1224:1230	These results indicate that CS-MSNs are promising candidates for pH-responsive drug delivery in cancer therapy.
24115568	8	44	theme	promising	1236:1244	arg1	candidates					1246:1255	promising candidates	1236:1255	promising candidates for pH-responsive drug delivery in cancer therapy	1236:1305	These results indicate that CS-MSNs are promising candidates for pH-responsive drug delivery in cancer therapy.
24115568	1	45	theme	pH-responsive	157:169	arg1	MSNs					220:223	MSNs	220:223	MSNs	220:223	Herein, we present a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs).
24115568	1	45	theme	pH-responsive	157:169	arg1	nanoparticles					205:217	pH-responsive chitosan-capped mesoporous silica nanoparticles	157:217	pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs)	157:224	Herein, we present a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs).
24115568	4	46	theme	drug	633:636	arg1	release					607:613	release	607:613	release	607:613	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	4	46	theme	drug	633:636	arg1	loading					595:601	loading	595:601	loading	595:601	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	0	47	theme	controlled	82:91	arg1	release					98:104	controlled drug release	82:104	controlled drug release	82:104	Chitosan-capped mesoporous silica nanoparticles as pH-responsive nanocarriers for controlled drug release.
24115568	6	48	theme	naked	1002:1006	arg1	MSNs					1008:1011	the naked MSNs	998:1011	the naked MSNs in an MTT assay	998:1027	Moreover, thanks to the perfect biocompatibility of chitosan, the CS-MSNs exhibited lower cytotoxicity than that of the naked MSNs in an MTT assay.
24115568	1	49	theme	chitosan-capped	171:185	arg1	MSNs					220:223	MSNs	220:223	MSNs	220:223	Herein, we present a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs).
24115568	1	49	theme	chitosan-capped	171:185	arg1	nanoparticles					205:217	pH-responsive chitosan-capped mesoporous silica nanoparticles	157:217	pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs)	157:224	Herein, we present a straightforward synthesis of pH-responsive chitosan-capped mesoporous silica nanoparticles (MSNs).
24115568	4	50	theme	anti-cancer	621:631	arg1	hydrochloride					651:663	doxorubicin hydrochloride	639:663	doxorubicin hydrochloride	639:663	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
24115568	4	50	theme	anti-cancer	621:631	arg1	drug					633:636	an anti-cancer drug	618:636	an anti-cancer drug	618:636	Owing to the pH-responsive and biocompatible features of chitosan, the loading and release of an anti-cancer drug, doxorubicin hydrochloride, were carried out in vitro, in which the composite chitosan-capped MSNs (CS-MSNs) showed excellent environmental response.
27966943	1	0	theme	refractive	159:168	arg1	errors					170:175	uncorrected refractive errors	147:175	uncorrected refractive errors	147:175	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	0	1	theme	Ophthalmic	64:73	arg1	Applications					75:86	Ophthalmic Applications	64:86	Ophthalmic Applications	64:86	Poly(vinyl alcohol) Hydrogels Reinforced with Nanocellulose for Ophthalmic Applications: General Characteristics and Optical Properties.
27966943	2	2	theme	varying	327:333	arg1	properties					351:360	varying physicochemical properties	327:360	varying physicochemical properties	327:360	Several hydrogels with varying physicochemical properties are in use to manufacture soft contact lenses.
27966943	0	3	theme	General	89:95	arg1	Characteristics					97:111	General Characteristics	89:111	General Characteristics	89:111	Poly(vinyl alcohol) Hydrogels Reinforced with Nanocellulose for Ophthalmic Applications: General Characteristics and Optical Properties.
27966943	3	4	theme	mechanical	551:560	arg1	properties					562:571	mechanical properties	551:571	mechanical properties	551:571	Hydrogels are generally too soft and reinforcement with appropriate materials is desirable to achieve high water content without compromising mechanical properties.
27966943	2	5	theme	physicochemical	335:349	arg1	properties					351:360	varying physicochemical properties	327:360	varying physicochemical properties	327:360	Several hydrogels with varying physicochemical properties are in use to manufacture soft contact lenses.
27966943	1	6	theme	important	253:261	arg1	part					263:266	an important part	250:266	an important part of modern day eye care and culture	250:301	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	4	7	theme	macroporous	628:638	arg1	hydrogel					640:647	a highly transparent macroporous hydrogel	607:647	a highly transparent macroporous hydrogel with water content >90%	607:671	In this study, we have developed a highly transparent macroporous hydrogel with water content >90%, by combining poly(vinyl alcohol) with nanocellulose.
27966943	4	8	theme	>90	668:670	arg1	%					671:671	%	671:671	%	671:671	In this study, we have developed a highly transparent macroporous hydrogel with water content >90%, by combining poly(vinyl alcohol) with nanocellulose.
27966943	5	9	theme	good	838:841	arg1	properties					855:864	very good UV-blocking properties	833:864	very good UV-blocking properties	833:864	Furthermore, the results show that the composite hydrogel has refractive index close to that of water and very good UV-blocking properties.
27966943	5	10	theme	refractive	789:798	arg1	index					800:804	refractive index	789:804	refractive index close to that of water and very good UV-blocking properties	789:864	Furthermore, the results show that the composite hydrogel has refractive index close to that of water and very good UV-blocking properties.
27966943	5	11	theme	UV-blocking	843:853	arg1	properties					855:864	very good UV-blocking properties	833:864	very good UV-blocking properties	833:864	Furthermore, the results show that the composite hydrogel has refractive index close to that of water and very good UV-blocking properties.
27966943	0	12	theme	Poly	0:3	arg1	Hydrogels					20:28	Poly(vinyl alcohol) Hydrogels	0:28	Poly(vinyl alcohol) Hydrogels Reinforced with Nanocellulose for Ophthalmic Applications: General Characteristics and Optical Properties.	0:135	Poly(vinyl alcohol) Hydrogels Reinforced with Nanocellulose for Ophthalmic Applications: General Characteristics and Optical Properties.
27966943	4	13	theme	%	671:671	arg1	content					660:666	water content	654:666	water content >90%	654:671	In this study, we have developed a highly transparent macroporous hydrogel with water content >90%, by combining poly(vinyl alcohol) with nanocellulose.
27966943	1	14	theme	main	192:195	arg1	causes					197:202	the main causes	188:202	the main causes of visual impairment	188:223	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	0	15	theme	vinyl	5:9	arg1	Poly					0:3	Poly	0:3	Poly(vinyl alcohol) Hydrogels Reinforced with Nanocellulose for Ophthalmic Applications: General Characteristics and Optical Properties.	0:135	Poly(vinyl alcohol) Hydrogels Reinforced with Nanocellulose for Ophthalmic Applications: General Characteristics and Optical Properties.
27966943	0	15	theme	vinyl	5:9	arg1	alcohol					11:17	vinyl alcohol	5:17	vinyl alcohol	5:17	Poly(vinyl alcohol) Hydrogels Reinforced with Nanocellulose for Ophthalmic Applications: General Characteristics and Optical Properties.
27966943	1	16	theme	modern	271:276	arg1	care					286:289	modern day eye care	271:289	modern day eye care	271:289	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	0	17	theme	Optical	117:123	arg1	Properties					125:134	Optical Properties	117:134	Optical Properties	117:134	Poly(vinyl alcohol) Hydrogels Reinforced with Nanocellulose for Ophthalmic Applications: General Characteristics and Optical Properties.
27966943	1	18	theme	causes	197:202	arg1	causes					197:202	the main causes	188:202	the main causes of visual impairment	188:223	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	1	18	theme	causes	197:202	arg1	one					181:183	one	181:183	one	181:183	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	1	19	theme	day	278:280	arg1	care					286:289	modern day eye care	271:289	modern day eye care	271:289	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	3	20	with	reinforcement	446:458	arg1	materials					477:485	appropriate materials	465:485	appropriate materials	465:485	Hydrogels are generally too soft and reinforcement with appropriate materials is desirable to achieve high water content without compromising mechanical properties.
27966943	4	21	theme	water	654:658	arg1	content					660:666	water content	654:666	water content >90%	654:671	In this study, we have developed a highly transparent macroporous hydrogel with water content >90%, by combining poly(vinyl alcohol) with nanocellulose.
27966943	2	22	theme	contact	393:399	arg1	lenses					401:406	soft contact lenses	388:406	soft contact lenses	388:406	Several hydrogels with varying physicochemical properties are in use to manufacture soft contact lenses.
27966943	5	23	theme	composite	766:774	arg1	hydrogel					776:783	the composite hydrogel	762:783	the composite hydrogel	762:783	Furthermore, the results show that the composite hydrogel has refractive index close to that of water and very good UV-blocking properties.
27966943	1	24	theme	eye	282:284	arg1	care					286:289	modern day eye care	271:289	modern day eye care	271:289	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	2	25	theme	soft	388:391	arg1	lenses					401:406	soft contact lenses	388:406	soft contact lenses	388:406	Several hydrogels with varying physicochemical properties are in use to manufacture soft contact lenses.
27966943	1	26	theme	care	286:289	arg1	part					263:266	an important part	250:266	an important part of modern day eye care and culture	250:301	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	2	27	with	hydrogels	312:320	arg1	properties					351:360	varying physicochemical properties	327:360	varying physicochemical properties	327:360	Several hydrogels with varying physicochemical properties are in use to manufacture soft contact lenses.
27966943	0	28	dep	Hydrogels	20:28	arg1	Characteristics					97:111	General Characteristics	89:111	General Characteristics	89:111	Poly(vinyl alcohol) Hydrogels Reinforced with Nanocellulose for Ophthalmic Applications: General Characteristics and Optical Properties.
27966943	0	28	dep	Hydrogels	20:28	arg1	Properties					125:134	Optical Properties	117:134	Optical Properties	117:134	Poly(vinyl alcohol) Hydrogels Reinforced with Nanocellulose for Ophthalmic Applications: General Characteristics and Optical Properties.
27966943	5	29	contain	has	785:787	arg1	hydrogel					776:783	the composite hydrogel	762:783	the composite hydrogel	762:783	Furthermore, the results show that the composite hydrogel has refractive index close to that of water and very good UV-blocking properties.
27966943	5	29	contain	has	785:787	arg2	index					800:804	refractive index	789:804	refractive index close to that of water and very good UV-blocking properties	789:864	Furthermore, the results show that the composite hydrogel has refractive index close to that of water and very good UV-blocking properties.
27966943	4	30	theme	vinyl	692:696	arg1	poly					687:690	poly	687:690	poly(vinyl alcohol)	687:705	In this study, we have developed a highly transparent macroporous hydrogel with water content >90%, by combining poly(vinyl alcohol) with nanocellulose.
27966943	4	30	theme	vinyl	692:696	arg1	alcohol					698:704	vinyl alcohol	692:704	vinyl alcohol	692:704	In this study, we have developed a highly transparent macroporous hydrogel with water content >90%, by combining poly(vinyl alcohol) with nanocellulose.
27966943	1	31	theme	culture	295:301	arg1	part					263:266	an important part	250:266	an important part of modern day eye care and culture	250:301	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	2	32	theme	Several	304:310	arg1	hydrogels					312:320	Several hydrogels	304:320	Several hydrogels with varying physicochemical properties	304:360	Several hydrogels with varying physicochemical properties are in use to manufacture soft contact lenses.
27966943	1	33	theme	impairment	214:223	arg1	causes					197:202	the main causes	188:202	the main causes of visual impairment	188:223	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	4	34	with	hydrogel	640:647	arg1	content					660:666	water content	654:666	water content >90%	654:671	In this study, we have developed a highly transparent macroporous hydrogel with water content >90%, by combining poly(vinyl alcohol) with nanocellulose.
27966943	3	35	theme	water	516:520	arg1	content					522:528	high water content	511:528	high water content	511:528	Hydrogels are generally too soft and reinforcement with appropriate materials is desirable to achieve high water content without compromising mechanical properties.
27966943	3	36	theme	appropriate	465:475	arg1	materials					477:485	appropriate materials	465:485	appropriate materials	465:485	Hydrogels are generally too soft and reinforcement with appropriate materials is desirable to achieve high water content without compromising mechanical properties.
27966943	1	37	theme	visual	207:212	arg1	impairment					214:223	visual impairment	207:223	visual impairment	207:223	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	3	38	theme	high	511:514	arg1	content					522:528	high water content	511:528	high water content	511:528	Hydrogels are generally too soft and reinforcement with appropriate materials is desirable to achieve high water content without compromising mechanical properties.
27966943	4	39	theme	transparent	616:626	arg1	hydrogel					640:647	a highly transparent macroporous hydrogel	607:647	a highly transparent macroporous hydrogel with water content >90%	607:671	In this study, we have developed a highly transparent macroporous hydrogel with water content >90%, by combining poly(vinyl alcohol) with nanocellulose.
27966943	1	40	theme	uncorrected	147:157	arg1	errors					170:175	uncorrected refractive errors	147:175	uncorrected refractive errors	147:175	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
27966943	1	41	theme	contact	230:236	arg1	lenses					238:243	contact lenses	230:243	contact lenses	230:243	Globally, uncorrected refractive errors are one of the main causes of visual impairment, and contact lenses form an important part of modern day eye care and culture.
25498685	5	0	theme	Cu	772:773	arg1	ions					779:782	Cu(2+) ions	772:782	Cu(2+) ions	772:782	An experimentally maximum adsorption of 89 mg/g was achieved for Cu(2+) ions.
25498685	4	1	theme	Zel/Chi	692:698	arg1	ratio					700:704	the Zel/Chi ratio	688:704	the Zel/Chi ratio	688:704	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	4	2	theme	monoliths	602:610	arg1	capacity					578:585	The metal ion adsorption capacity	553:585	The metal ion adsorption capacity of the Zel/Chi monoliths	553:610	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	4	2	theme	monoliths	602:610	arg1	related					616:622	related	616:622	related	616:622	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	4	3	theme	Zel/Chi	594:600	arg1	monoliths					602:610	the Zel/Chi monoliths	590:610	the Zel/Chi monoliths	590:610	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	0	4	theme	metal	93:97	arg1	ions					99:102	metal ions	93:102	metal ions	93:102	Fabrication of porous zeolite/chitosan monoliths and their applications for drug release and metal ions adsorption.
25498685	6	5	theme	4-nitrophenol	872:884	arg1	reduction					859:867	the reduction	855:867	the reduction of 4-nitrophenol to 4-aminophenol	855:901	The Zel/Chi monoliths with adsorbed Cu(2+) ions effectively catalyzed the reduction of 4-nitrophenol to 4-aminophenol and had good recyclability.
25498685	3	6	dep	composition	502:512	arg1	the					498:500	the	498:500	the	498:500	The release rate of cefalexin from drug-loaded Zel/Chi monoliths depended on the composition and porous structure of the monoliths.
25498685	6	7	theme	Zel/Chi	789:795	arg1	monoliths					797:805	The Zel/Chi monoliths	785:805	The Zel/Chi monoliths with adsorbed Cu(2+) ions	785:831	The Zel/Chi monoliths with adsorbed Cu(2+) ions effectively catalyzed the reduction of 4-nitrophenol to 4-aminophenol and had good recyclability.
25498685	0	8	dep	adsorption	104:113	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of porous zeolite/chitosan monoliths and their applications for drug release and metal ions	0:102	Fabrication of porous zeolite/chitosan monoliths and their applications for drug release and metal ions adsorption.
25498685	1	9	theme	various	282:288	arg1	methods					303:309	various instrumental methods	282:309	various instrumental methods	282:309	Ordered porous zeolite/chitosan (Zel/Chi) monoliths were prepared by a unidirectional freeze-drying method, and their properties and structures were characterized by various instrumental methods.
25498685	5	10	theme	mg/g	750:753	arg1	adsorption					733:742	An experimentally maximum adsorption	707:742	An experimentally maximum adsorption of 89 mg/g	707:753	An experimentally maximum adsorption of 89 mg/g was achieved for Cu(2+) ions.
25498685	1	11	theme	Ordered	116:122	arg1	monoliths					158:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths	116:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths	116:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths were prepared by a unidirectional freeze-drying method, and their properties and structures were characterized by various instrumental methods.
25498685	7	12	with	rinsing	1011:1017	arg1	water					1029:1033	water	1029:1033	water	1029:1033	They were easily recovered by simply removing them from the reaction system and rinsing them with water.
25498685	4	13	theme	adsorption	668:677	arg1	time					679:682	the adsorption time	664:682	the adsorption time	664:682	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	3	14	theme	cefalexin	441:449	arg1	rate					433:436	The release rate	421:436	The release rate of cefalexin from drug-loaded Zel/Chi monoliths	421:484	The release rate of cefalexin from drug-loaded Zel/Chi monoliths depended on the composition and porous structure of the monoliths.
25498685	3	15	theme	drug-loaded	456:466	arg1	monoliths					476:484	drug-loaded Zel/Chi monoliths	456:484	drug-loaded Zel/Chi monoliths	456:484	The release rate of cefalexin from drug-loaded Zel/Chi monoliths depended on the composition and porous structure of the monoliths.
25498685	6	16	contain	had	907:909	arg1	monoliths					797:805	The Zel/Chi monoliths	785:805	The Zel/Chi monoliths with adsorbed Cu(2+) ions	785:831	The Zel/Chi monoliths with adsorbed Cu(2+) ions effectively catalyzed the reduction of 4-nitrophenol to 4-aminophenol and had good recyclability.
25498685	6	16	contain	had	907:909	arg2	recyclability					916:928	good recyclability	911:928	good recyclability	911:928	The Zel/Chi monoliths with adsorbed Cu(2+) ions effectively catalyzed the reduction of 4-nitrophenol to 4-aminophenol and had good recyclability.
25498685	1	17	theme	instrumental	290:301	arg1	methods					303:309	various instrumental methods	282:309	various instrumental methods	282:309	Ordered porous zeolite/chitosan (Zel/Chi) monoliths were prepared by a unidirectional freeze-drying method, and their properties and structures were characterized by various instrumental methods.
25498685	6	18	theme	good	911:914	arg1	recyclability					916:928	good recyclability	911:928	good recyclability	911:928	The Zel/Chi monoliths with adsorbed Cu(2+) ions effectively catalyzed the reduction of 4-nitrophenol to 4-aminophenol and had good recyclability.
25498685	2	19	theme	monoliths	392:400	arg1	ion					322:324	The metal ion adsorption and the drug release performance of the porous Zel/Chi monoliths	312:400	ion	322:324	The metal ion adsorption and the drug release performance of the porous Zel/Chi monoliths were also studied.
25498685	2	19	theme	monoliths	392:400	arg1	performance					358:368	the drug release performance	341:368	the drug release performance of the porous Zel/Chi monoliths	341:400	The metal ion adsorption and the drug release performance of the porous Zel/Chi monoliths were also studied.
25498685	0	20	theme	zeolite/chitosan	22:37	arg1	monoliths					39:47	porous zeolite/chitosan monoliths	15:47	porous zeolite/chitosan monoliths	15:47	Fabrication of porous zeolite/chitosan monoliths and their applications for drug release and metal ions adsorption.
25498685	4	21	theme	adsorption	567:576	arg1	capacity					578:585	The metal ion adsorption capacity	553:585	The metal ion adsorption capacity of the Zel/Chi monoliths	553:610	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	4	21	theme	adsorption	567:576	arg1	related					616:622	related	616:622	related	616:622	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	1	22	theme	freeze-drying	202:214	arg1	method					216:221	a unidirectional freeze-drying method	185:221	a unidirectional freeze-drying method	185:221	Ordered porous zeolite/chitosan (Zel/Chi) monoliths were prepared by a unidirectional freeze-drying method, and their properties and structures were characterized by various instrumental methods.
25498685	0	23	theme	porous	15:20	arg1	monoliths					39:47	porous zeolite/chitosan monoliths	15:47	porous zeolite/chitosan monoliths	15:47	Fabrication of porous zeolite/chitosan monoliths and their applications for drug release and metal ions adsorption.
25498685	2	24	theme	metal	316:320	arg1	ion					322:324	The metal ion adsorption and the drug release performance of the porous Zel/Chi monoliths	312:400	ion	322:324	The metal ion adsorption and the drug release performance of the porous Zel/Chi monoliths were also studied.
25498685	1	25	theme	Zel/Chi	149:155	arg1	monoliths					158:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths	116:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths	116:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths were prepared by a unidirectional freeze-drying method, and their properties and structures were characterized by various instrumental methods.
25498685	3	26	theme	release	425:431	arg1	rate					433:436	The release rate	421:436	The release rate of cefalexin from drug-loaded Zel/Chi monoliths	421:484	The release rate of cefalexin from drug-loaded Zel/Chi monoliths depended on the composition and porous structure of the monoliths.
25498685	2	27	theme	release	350:356	arg1	performance					358:368	the drug release performance	341:368	the drug release performance of the porous Zel/Chi monoliths	341:400	The metal ion adsorption and the drug release performance of the porous Zel/Chi monoliths were also studied.
25498685	5	28	theme	maximum	725:731	arg1	adsorption					733:742	An experimentally maximum adsorption	707:742	An experimentally maximum adsorption of 89 mg/g	707:753	An experimentally maximum adsorption of 89 mg/g was achieved for Cu(2+) ions.
25498685	1	29	theme	unidirectional	187:200	arg1	method					216:221	a unidirectional freeze-drying method	185:221	a unidirectional freeze-drying method	185:221	Ordered porous zeolite/chitosan (Zel/Chi) monoliths were prepared by a unidirectional freeze-drying method, and their properties and structures were characterized by various instrumental methods.
25498685	3	30	theme	Zel/Chi	468:474	arg1	monoliths					476:484	drug-loaded Zel/Chi monoliths	456:484	drug-loaded Zel/Chi monoliths	456:484	The release rate of cefalexin from drug-loaded Zel/Chi monoliths depended on the composition and porous structure of the monoliths.
25498685	0	31	theme	monoliths	39:47	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of porous zeolite/chitosan monoliths and their applications for drug release and metal ions	0:102	Fabrication of porous zeolite/chitosan monoliths and their applications for drug release and metal ions adsorption.
25498685	4	32	theme	ions	658:661	arg1	ratio					700:704	the Zel/Chi ratio	688:704	the Zel/Chi ratio	688:704	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	4	32	theme	ions	658:661	arg1	time					679:682	the adsorption time	664:682	the adsorption time	664:682	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	4	32	theme	ions	658:661	arg1	concentration					631:643	the concentration	627:643	the concentration of the metal ions	627:661	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	0	33	theme	applications	59:70	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of porous zeolite/chitosan monoliths and their applications for drug release and metal ions	0:102	Fabrication of porous zeolite/chitosan monoliths and their applications for drug release and metal ions adsorption.
25498685	3	34	from	monoliths	476:484	arg1	rate					433:436	The release rate	421:436	The release rate of cefalexin from drug-loaded Zel/Chi monoliths	421:484	The release rate of cefalexin from drug-loaded Zel/Chi monoliths depended on the composition and porous structure of the monoliths.
25498685	2	35	dep	ion	322:324	arg1	adsorption					326:335	adsorption	326:335	adsorption	326:335	The metal ion adsorption and the drug release performance of the porous Zel/Chi monoliths were also studied.
25498685	4	36	theme	ion	563:565	arg1	capacity					578:585	The metal ion adsorption capacity	553:585	The metal ion adsorption capacity of the Zel/Chi monoliths	553:610	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	4	36	theme	ion	563:565	arg1	related					616:622	related	616:622	related	616:622	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	1	37	theme	porous	124:129	arg1	monoliths					158:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths	116:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths	116:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths were prepared by a unidirectional freeze-drying method, and their properties and structures were characterized by various instrumental methods.
25498685	4	38	theme	metal	557:561	arg1	capacity					578:585	The metal ion adsorption capacity	553:585	The metal ion adsorption capacity of the Zel/Chi monoliths	553:610	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	4	38	theme	metal	557:561	arg1	related					616:622	related	616:622	related	616:622	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	7	39	theme	reaction	991:998	arg1	system					1000:1005	the reaction system	987:1005	the reaction system	987:1005	They were easily recovered by simply removing them from the reaction system and rinsing them with water.
25498685	1	40	theme	zeolite/chitosan	131:146	arg1	monoliths					158:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths	116:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths	116:166	Ordered porous zeolite/chitosan (Zel/Chi) monoliths were prepared by a unidirectional freeze-drying method, and their properties and structures were characterized by various instrumental methods.
25498685	6	41	theme	Cu	821:822	arg1	ions					828:831	adsorbed Cu(2+) ions	812:831	adsorbed Cu(2+) ions	812:831	The Zel/Chi monoliths with adsorbed Cu(2+) ions effectively catalyzed the reduction of 4-nitrophenol to 4-aminophenol and had good recyclability.
25498685	3	42	theme	monoliths	542:550	arg1	composition					502:512	composition	502:512	composition	502:512	The release rate of cefalexin from drug-loaded Zel/Chi monoliths depended on the composition and porous structure of the monoliths.
25498685	3	42	theme	monoliths	542:550	arg1	structure					525:533	porous structure	518:533	porous structure	518:533	The release rate of cefalexin from drug-loaded Zel/Chi monoliths depended on the composition and porous structure of the monoliths.
25498685	2	43	theme	drug	345:348	arg1	performance					358:368	the drug release performance	341:368	the drug release performance of the porous Zel/Chi monoliths	341:400	The metal ion adsorption and the drug release performance of the porous Zel/Chi monoliths were also studied.
25498685	0	44	theme	drug	76:79	arg1	release					81:87	drug release	76:87	drug release	76:87	Fabrication of porous zeolite/chitosan monoliths and their applications for drug release and metal ions adsorption.
25498685	6	45	theme	adsorbed	812:819	arg1	2+					824:825	2+	824:825	2+	824:825	The Zel/Chi monoliths with adsorbed Cu(2+) ions effectively catalyzed the reduction of 4-nitrophenol to 4-aminophenol and had good recyclability.
25498685	6	45	theme	adsorbed	812:819	arg1	Cu					821:822	adsorbed Cu	812:822	adsorbed Cu(2+) ions	812:831	The Zel/Chi monoliths with adsorbed Cu(2+) ions effectively catalyzed the reduction of 4-nitrophenol to 4-aminophenol and had good recyclability.
25498685	4	46	theme	metal	652:656	arg1	ions					658:661	the metal ions	648:661	the metal ions	648:661	The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio.
25498685	2	47	theme	Zel/Chi	384:390	arg1	monoliths					392:400	the porous Zel/Chi monoliths	373:400	the porous Zel/Chi monoliths	373:400	The metal ion adsorption and the drug release performance of the porous Zel/Chi monoliths were also studied.
25498685	6	48	with	monoliths	797:805	arg1	ions					828:831	adsorbed Cu(2+) ions	812:831	adsorbed Cu(2+) ions	812:831	The Zel/Chi monoliths with adsorbed Cu(2+) ions effectively catalyzed the reduction of 4-nitrophenol to 4-aminophenol and had good recyclability.
25498685	2	49	theme	porous	377:382	arg1	monoliths					392:400	the porous Zel/Chi monoliths	373:400	the porous Zel/Chi monoliths	373:400	The metal ion adsorption and the drug release performance of the porous Zel/Chi monoliths were also studied.
25498685	3	50	theme	porous	518:523	arg1	structure					525:533	porous structure	518:533	porous structure	518:533	The release rate of cefalexin from drug-loaded Zel/Chi monoliths depended on the composition and porous structure of the monoliths.
26320017	3	0	theme	cell	662:665	arg1	nature					646:651	the nature	642:651	the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry	642:728	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	3	0	theme	cell	662:665	arg1	function					771:778	a function	769:778	a function of time	769:786	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	1	1	theme	temperature	138:148	arg1	pretreatment					184:195	high temperature water-only continuous flowthrough pretreatment	133:195	high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR)	133:241	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	1	theme	temperature	138:148	arg1	tool					269:272	a promising analytical tool	246:272	a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass	246:458	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	4	2	theme	chemical	985:992	arg1	composition					994:1004	chemical composition	985:1004	chemical composition	985:1004	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	3	3	theme	time	783:786	arg1	nature					646:651	the nature	642:651	the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry	642:728	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	3	3	theme	time	783:786	arg1	function					771:778	a function	769:778	a function of time	769:786	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	0	4	theme	13	81:82	arg1	C					84:84	C	84:84	C	84:84	Recalcitrance and structural analysis by water-only flowthrough pretreatment of (13)C enriched corn stover stem.
26320017	4	5	theme	effluent	814:821	arg1	Fractions					789:797	Fractions	789:797	Fractions of the reactor effluent	789:821	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	2	6	theme	wall	605:608	arg1	fractionation					579:591	fractionation	579:591	fractionation of the cell wall	579:608	(13)C-enriched corn stover stems were pretreated at 170°C for 60min with a hot-water flow rate of 20mL/min to control fractionation of the cell wall.
26320017	1	7	theme	water-only	150:159	arg1	pretreatment					184:195	high temperature water-only continuous flowthrough pretreatment	133:195	high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR)	133:241	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	7	theme	water-only	150:159	arg1	tool					269:272	a promising analytical tool	246:272	a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass	246:458	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	4	8	theme	molecular	1007:1015	arg1	structure					1017:1025	molecular structure	1007:1025	molecular structure	1007:1025	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	1	9	theme	promising	248:256	arg1	pretreatment					184:195	high temperature water-only continuous flowthrough pretreatment	133:195	high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR)	133:241	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	9	theme	promising	248:256	arg1	tool					269:272	a promising analytical tool	246:272	a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass	246:458	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	0	10	theme	enriched	86:93	arg1	stem					107:110	(13)C enriched corn stover stem	80:110	(13)C enriched corn stover stem	80:110	Recalcitrance and structural analysis by water-only flowthrough pretreatment of (13)C enriched corn stover stem.
26320017	2	11	theme	flow	546:549	arg1	rate					551:554	a hot-water flow rate	534:554	a hot-water flow rate of 20mL/min to control fractionation of the cell wall	534:608	(13)C-enriched corn stover stems were pretreated at 170°C for 60min with a hot-water flow rate of 20mL/min to control fractionation of the cell wall.
26320017	2	12	theme	cell	600:603	arg1	wall					605:608	the cell wall	596:608	the cell wall	596:608	(13)C-enriched corn stover stems were pretreated at 170°C for 60min with a hot-water flow rate of 20mL/min to control fractionation of the cell wall.
26320017	1	13	theme	analytical	258:267	arg1	pretreatment					184:195	high temperature water-only continuous flowthrough pretreatment	133:195	high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR)	133:241	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	13	theme	analytical	258:267	arg1	tool					269:272	a promising analytical tool	246:272	a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass	246:458	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	0	14	theme	C	84:84	arg1	stem					107:110	(13)C enriched corn stover stem	80:110	(13)C enriched corn stover stem	80:110	Recalcitrance and structural analysis by water-only flowthrough pretreatment of (13)C enriched corn stover stem.
26320017	3	15	theme	wall	667:670	arg1	recalcitrance					681:693	wall chemical recalcitrance	667:693	wall chemical recalcitrance	667:693	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	2	16	theme	hot-water	536:544	arg1	rate					551:554	a hot-water flow rate	534:554	a hot-water flow rate of 20mL/min to control fractionation of the cell wall	534:608	(13)C-enriched corn stover stems were pretreated at 170°C for 60min with a hot-water flow rate of 20mL/min to control fractionation of the cell wall.
26320017	4	17	dep	composition	994:1004	arg1	the					981:983	the	981:983	the	981:983	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	3	18	dep	cell	662:665	arg1	recalcitrance					681:693	wall chemical recalcitrance	667:693	wall chemical recalcitrance	667:693	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	0	19	theme	stover	100:105	arg1	stem					107:110	(13)C enriched corn stover stem	80:110	(13)C enriched corn stover stem	80:110	Recalcitrance and structural analysis by water-only flowthrough pretreatment of (13)C enriched corn stover stem.
26320017	3	20	theme	chemical	672:679	arg1	recalcitrance					681:693	wall chemical recalcitrance	667:693	wall chemical recalcitrance	667:693	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	4	21	theme	released	1067:1074	arg1	fragments					1076:1084	those released fragments	1061:1084	those released fragments	1061:1084	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	1	22	theme	continuous	161:170	arg1	pretreatment					184:195	high temperature water-only continuous flowthrough pretreatment	133:195	high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR)	133:241	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	22	theme	continuous	161:170	arg1	tool					269:272	a promising analytical tool	246:272	a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass	246:458	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	0	23	theme	corn	95:98	arg1	stem					107:110	(13)C enriched corn stover stem	80:110	(13)C enriched corn stover stem	80:110	Recalcitrance and structural analysis by water-only flowthrough pretreatment of (13)C enriched corn stover stem.
26320017	1	24	theme	cell	345:348	arg1	resistance					355:364	cell wall resistance	345:364	cell wall resistance to deconstruction	345:382	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	25	theme	batch	430:434	arg1	pretreatment					436:447	batch pretreatment	430:447	batch pretreatment of biomass	430:458	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	26	theme	flowthrough	172:182	arg1	pretreatment					184:195	high temperature water-only continuous flowthrough pretreatment	133:195	high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR)	133:241	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	26	theme	flowthrough	172:182	arg1	tool					269:272	a promising analytical tool	246:272	a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass	246:458	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	27	theme	wall	350:353	arg1	resistance					355:364	cell wall resistance	345:364	cell wall resistance to deconstruction	345:382	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	4	28	theme	reactor	806:812	arg1	effluent					814:821	the reactor effluent	802:821	the reactor effluent	802:821	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	3	29	theme	biomass	699:705	arg1	chemistry					720:728	biomass pretreatment chemistry	699:728	biomass pretreatment chemistry	699:728	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	2	30	theme	20mL/min	559:566	arg1	rate					551:554	a hot-water flow rate	534:554	a hot-water flow rate of 20mL/min to control fractionation of the cell wall	534:608	(13)C-enriched corn stover stems were pretreated at 170°C for 60min with a hot-water flow rate of 20mL/min to control fractionation of the cell wall.
26320017	3	31	theme	cell	742:745	arg1	fragmentation					752:764	tracking cell wall fragmentation	733:764	tracking cell wall fragmentation	733:764	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	3	32	theme	pretreatment	707:718	arg1	chemistry					720:728	biomass pretreatment chemistry	699:728	biomass pretreatment chemistry	699:728	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	4	33	theme	fragments	1076:1084	arg1	composition					994:1004	chemical composition	985:1004	chemical composition	985:1004	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	4	33	theme	fragments	1076:1084	arg1	structure					1017:1025	molecular structure	1007:1025	molecular structure	1007:1025	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	4	33	theme	fragments	1076:1084	arg1	weight					1051:1056	relative molecular weight	1032:1056	relative molecular weight	1032:1056	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	1	34	theme	biomass	452:458	arg1	pretreatment					436:447	batch pretreatment	430:447	batch pretreatment of biomass	430:458	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	4	35	theme	molecular	1041:1049	arg1	weight					1051:1056	relative molecular weight	1032:1056	relative molecular weight	1032:1056	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	0	36	theme	structural	18:27	arg1	analysis					29:36	structural analysis	18:36	structural analysis	18:36	Recalcitrance and structural analysis by water-only flowthrough pretreatment of (13)C enriched corn stover stem.
26320017	0	37	theme	stem	107:110	arg1	pretreatment					64:75	water-only flowthrough pretreatment	41:75	water-only flowthrough pretreatment of (13)C enriched corn stover stem	41:110	Recalcitrance and structural analysis by water-only flowthrough pretreatment of (13)C enriched corn stover stem.
26320017	1	38	theme	nuclear	210:216	arg1	NMR					238:240	NMR	238:240	NMR	238:240	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	38	theme	nuclear	210:216	arg1	resonance					227:235	nuclear magnetic resonance	210:235	nuclear magnetic resonance (NMR)	210:241	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	0	39	theme	water-only	41:50	arg1	pretreatment					64:75	water-only flowthrough pretreatment	41:75	water-only flowthrough pretreatment of (13)C enriched corn stover stem	41:110	Recalcitrance and structural analysis by water-only flowthrough pretreatment of (13)C enriched corn stover stem.
26320017	1	40	theme	plant	289:293	arg1	wall					300:303	the plant cell wall	285:303	the plant cell wall	285:303	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	4	41	theme	NMR	894:896	arg1	techniques					898:907	various NMR techniques	886:907	various NMR techniques	886:907	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	1	42	theme	magnetic	218:225	arg1	NMR					238:240	NMR	238:240	NMR	238:240	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	42	theme	magnetic	218:225	arg1	resonance					227:235	nuclear magnetic resonance	210:235	nuclear magnetic resonance (NMR)	210:241	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	4	43	theme	fragments	949:957	arg1	sequence					937:944	sequence	937:944	sequence	937:944	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	4	43	theme	fragments	949:957	arg1	degree					926:931	the degree	922:931	the degree	922:931	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	1	44	theme	cell	295:298	arg1	wall					300:303	the plant cell wall	285:303	the plant cell wall	285:303	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	4	45	theme	various	886:892	arg1	techniques					898:907	various NMR techniques	886:907	various NMR techniques	886:907	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	3	46	theme	wall	747:750	arg1	fragmentation					752:764	tracking cell wall fragmentation	733:764	tracking cell wall fragmentation	733:764	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	2	47	theme	C-enriched	465:474	arg1	stems					488:492	(13)C-enriched corn stover stems	461:492	(13)C-enriched corn stover stems	461:492	(13)C-enriched corn stover stems were pretreated at 170°C for 60min with a hot-water flow rate of 20mL/min to control fractionation of the cell wall.
26320017	3	48	theme	tracking	733:740	arg1	fragmentation					752:764	tracking cell wall fragmentation	733:764	tracking cell wall fragmentation	733:764	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	4	49	theme	time-resolved	843:855	arg1	fashion					857:863	a time-resolved fashion	841:863	a time-resolved fashion	841:863	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	3	50	theme	plant	656:660	arg1	cell					662:665	plant cell wall chemical recalcitrance and biomass pretreatment chemistry	656:728	cell	662:665	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	0	51	theme	flowthrough	52:62	arg1	pretreatment					64:75	water-only flowthrough pretreatment	41:75	water-only flowthrough pretreatment of (13)C enriched corn stover stem	41:110	Recalcitrance and structural analysis by water-only flowthrough pretreatment of (13)C enriched corn stover stem.
26320017	3	52	theme	chemistry	720:728	arg1	nature					646:651	the nature	642:651	the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry	642:728	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	3	52	theme	chemistry	720:728	arg1	function					771:778	a function	769:778	a function of time	769:786	This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function of time.
26320017	1	53	dep	tool	269:272	arg1	understand					309:318	understand	309:318	to understand its recalcitrance (i.e., cell wall resistance to deconstruction)	306:383	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	53	dep	tool	269:272	arg1	examine					277:283	examine	277:283	to examine the plant cell wall	274:303	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	53	dep	tool	269:272	arg1	probe					393:397	probe	393:397	to probe the chemistry occurring during batch pretreatment of biomass	390:458	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	4	54	theme	relative	1032:1039	arg1	weight					1051:1056	relative molecular weight	1032:1056	relative molecular weight	1032:1056	Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.
26320017	2	55	theme	corn	476:479	arg1	stems					488:492	(13)C-enriched corn stover stems	461:492	(13)C-enriched corn stover stems	461:492	(13)C-enriched corn stover stems were pretreated at 170°C for 60min with a hot-water flow rate of 20mL/min to control fractionation of the cell wall.
26320017	1	56	dep	resistance	355:364	arg1	i.e.					339:342	i.e.	339:342	i.e.	339:342	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	57	theme	high	133:136	arg1	pretreatment					184:195	high temperature water-only continuous flowthrough pretreatment	133:195	high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR)	133:241	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	1	57	theme	high	133:136	arg1	tool					269:272	a promising analytical tool	246:272	a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass	246:458	This study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass.
26320017	2	58	theme	stover	481:486	arg1	stems					488:492	(13)C-enriched corn stover stems	461:492	(13)C-enriched corn stover stems	461:492	(13)C-enriched corn stover stems were pretreated at 170°C for 60min with a hot-water flow rate of 20mL/min to control fractionation of the cell wall.
28754911	0	0	theme	gut	84:86	arg1	microbiome					88:97	the murine gut microbiome	73:97	the murine gut microbiome	73:97	Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions.
28754911	6	1	theme	%	924:924	arg1	abundance					898:906	the abundance	894:906	the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively	894:1026	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	4	2	theme	fecal	591:595	arg1	acids					615:619	total fecal short-chain fatty acids	585:619	total fecal short-chain fatty acids from the level significantly repressed in mice under the stress	585:683	CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress.
28754911	0	3	theme	murine	77:82	arg1	microbiome					88:97	the murine gut microbiome	73:97	the murine gut microbiome	73:97	Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions.
28754911	6	4	theme	profound	838:845	arg1	impact					847:852	a profound impact	836:852	a profound impact	836:852	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	2	5	theme	bioactive	333:341	arg1	composition					379:389	gut microbial composition	365:389	gut microbial composition in mice	365:397	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	2	5	theme	bioactive	333:341	arg1	compound					343:350	a bioactive compound	331:350	a bioactive compound	331:350	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	2	5	theme	bioactive	333:341	arg1	disaccharides					306:318	CS disaccharides	303:318	CS disaccharides	303:318	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	4	6	theme	total	585:589	arg1	acids					615:619	total fecal short-chain fatty acids	585:619	total fecal short-chain fatty acids from the level significantly repressed in mice under the stress	585:683	CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress.
28754911	8	7	theme	intestinal	1175:1184	arg1	inflammation					1186:1197	stress-induced intestinal inflammation	1160:1197	stress-induced intestinal inflammation	1160:1197	Together, our findings demonstrated that CS may ameliorate stress-induced intestinal inflammation.
28754911	10	8	contain	had	1371:1373	arg1	disaccharides					1357:1369	CS disaccharides	1354:1369	CS disaccharides	1354:1369	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	10	8	contain	had	1371:1373	arg2	impact					1389:1394	a significant impact	1375:1394	a significant impact	1375:1394	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	9	9	theme	intestinal	1240:1249	arg1	population					1276:1285	intestinal Bacteroides acidifaciens population	1240:1285	intestinal Bacteroides acidifaciens population	1240:1285	Furthermore, CS significantly increased intestinal Bacteroides acidifaciens population, indirectly exerting its immunomodulatory effect on the intestine.
28754911	4	10	theme	fatty	609:613	arg1	acids					615:619	total fecal short-chain fatty acids	585:619	total fecal short-chain fatty acids from the level significantly repressed in mice under the stress	585:683	CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress.
28754911	0	11	theme	microbiome	88:97	arg1	structure					47:55	structure	47:55	structure	47:55	Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions.
28754911	0	11	theme	microbiome	88:97	arg1	function					61:68	function	61:68	function	61:68	Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions.
28754911	6	12	theme	%	915:915	arg1	abundance					898:906	the abundance	894:906	the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively	894:1026	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	9	13	theme	Bacteroides	1251:1261	arg1	population					1276:1285	intestinal Bacteroides acidifaciens population	1240:1285	intestinal Bacteroides acidifaciens population	1240:1285	Furthermore, CS significantly increased intestinal Bacteroides acidifaciens population, indirectly exerting its immunomodulatory effect on the intestine.
28754911	10	14	theme	significant	1377:1387	arg1	impact					1389:1394	a significant impact	1375:1394	a significant impact	1375:1394	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	1	15	theme	tissue	255:260	arg1	integrity					273:281	tissue structural integrity	255:281	tissue structural integrity	255:281	Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity.
28754911	9	16	theme	acidifaciens	1263:1274	arg1	population					1276:1285	intestinal Bacteroides acidifaciens population	1240:1285	intestinal Bacteroides acidifaciens population	1240:1285	Furthermore, CS significantly increased intestinal Bacteroides acidifaciens population, indirectly exerting its immunomodulatory effect on the intestine.
28754911	3	17	theme	exhaustive	521:530	arg1	stress					541:546	exhaustive exercise stress	521:546	exhaustive exercise stress	521:546	Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress.
28754911	3	18	theme	CS	419:420	arg1	supplementation					436:450	CS disaccharides supplementation	419:450	CS disaccharides supplementation for 16 days	419:462	Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress.
28754911	0	19	theme	healthy	105:111	arg1	conditions					126:135	healthy and stressed conditions	105:135	healthy and stressed conditions	105:135	Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions.
28754911	6	20	theme	CS	815:816	arg1	disaccharides					818:830	CS disaccharides	815:830	CS disaccharides	815:830	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	3	21	theme	exercise	532:539	arg1	stress					541:546	exhaustive exercise stress	521:546	exhaustive exercise stress	521:546	Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress.
28754911	3	22	theme	disaccharides	422:434	arg1	supplementation					436:450	CS disaccharides supplementation	419:450	CS disaccharides supplementation for 16 days	419:462	Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress.
28754911	5	23	theme	fecal	785:789	arg1	concentration					800:812	fecal butyrate concentration	785:812	fecal butyrate concentration	785:812	Our findings demonstrated that CS was likely butyrogenic and resulted in a significant increase in fecal butyrate concentration.
28754911	5	24	theme	significant	761:771	arg1	increase					773:780	a significant increase	759:780	a significant increase in fecal butyrate concentration	759:812	Our findings demonstrated that CS was likely butyrogenic and resulted in a significant increase in fecal butyrate concentration.
28754911	2	25	theme	microbial	369:377	arg1	composition					379:389	gut microbial composition	365:389	gut microbial composition in mice	365:397	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	2	25	theme	microbial	369:377	arg1	compound					343:350	a bioactive compound	331:350	a bioactive compound	331:350	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	2	25	theme	microbial	369:377	arg1	disaccharides					306:318	CS disaccharides	303:318	CS disaccharides	303:318	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	0	26	theme	Chondroitin	0:10	arg1	sulfate					12:18	Chondroitin sulfate	0:18	Chondroitin sulfate	0:18	Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions.
28754911	1	27	theme	structural	262:271	arg1	integrity					273:281	tissue structural integrity	255:281	tissue structural integrity	255:281	Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity.
28754911	10	28	theme	two-component	1488:1500	arg1	systems					1502:1508	two-component systems	1488:1508	two-component systems	1488:1508	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	2	29	theme	CS	303:304	arg1	composition					379:389	gut microbial composition	365:389	gut microbial composition in mice	365:397	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	2	29	theme	CS	303:304	arg1	compound					343:350	a bioactive compound	331:350	a bioactive compound	331:350	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	2	29	theme	CS	303:304	arg1	disaccharides					306:318	CS disaccharides	303:318	CS disaccharides	303:318	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	10	30	theme	broad	1401:1405	arg1	metabolism					1528:1537	carbohydrate metabolism	1515:1537	carbohydrate metabolism	1515:1537	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	10	30	theme	broad	1401:1405	arg1	range					1407:1411	a broad range	1399:1411	a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism	1399:1537	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	10	30	theme	broad	1401:1405	arg1	transporters					1474:1485	ABC transporters	1470:1485	ABC transporters	1470:1485	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	10	30	theme	broad	1401:1405	arg1	systems					1502:1508	two-component systems	1488:1508	two-component systems	1488:1508	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	2	31	theme	gut	365:367	arg1	composition					379:389	gut microbial composition	365:389	gut microbial composition in mice	365:397	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	2	31	theme	gut	365:367	arg1	compound					343:350	a bioactive compound	331:350	a bioactive compound	331:350	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	2	31	theme	gut	365:367	arg1	disaccharides					306:318	CS disaccharides	303:318	CS disaccharides	303:318	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	4	32	theme	CS	549:550	arg1	disaccharides					552:564	CS disaccharides	549:564	CS disaccharides	549:564	CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress.
28754911	6	33	from	communities	973:983	arg1	mice					1009:1012	healthy and stressed mice	988:1012	healthy and stressed mice	988:1012	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	0	34	theme	stressed	117:124	arg1	conditions					126:135	healthy and stressed conditions	105:135	healthy and stressed conditions	105:135	Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions.
28754911	1	35	theme	medical	188:194	arg1	purposes					214:221	medical and nutraceutical purposes	188:221	medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity	188:281	Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity.
28754911	5	36	theme	butyrate	791:798	arg1	concentration					800:812	fecal butyrate concentration	785:812	fecal butyrate concentration	785:812	Our findings demonstrated that CS was likely butyrogenic and resulted in a significant increase in fecal butyrate concentration.
28754911	6	37	theme	microbial	963:971	arg1	communities					973:983	fecal microbial communities	957:983	fecal microbial communities in healthy and stressed mice	957:1012	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	7	38	theme	inflammatory	1072:1083	arg1	Proteobacteria					1085:1098	inflammatory Proteobacteria	1072:1098	inflammatory Proteobacteria	1072:1098	CS disaccharides reduced the prevalence of inflammatory Proteobacteria.
28754911	9	39	theme	immunomodulatory	1312:1327	arg1	effect					1329:1334	its immunomodulatory effect	1308:1334	its immunomodulatory effect on the intestine	1308:1351	Furthermore, CS significantly increased intestinal Bacteroides acidifaciens population, indirectly exerting its immunomodulatory effect on the intestine.
28754911	6	40	theme	fecal	957:961	arg1	communities					973:983	fecal microbial communities	957:983	fecal microbial communities in healthy and stressed mice	957:1012	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	7	41	theme	Proteobacteria	1085:1098	arg1	prevalence					1058:1067	the prevalence	1054:1067	the prevalence of inflammatory Proteobacteria	1054:1098	CS disaccharides reduced the prevalence of inflammatory Proteobacteria.
28754911	7	42	theme	CS	1029:1030	arg1	disaccharides					1032:1044	CS disaccharides	1029:1044	CS disaccharides	1029:1044	CS disaccharides reduced the prevalence of inflammatory Proteobacteria.
28754911	1	43	theme	nutraceutical	200:212	arg1	purposes					214:221	medical and nutraceutical purposes	188:221	medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity	188:281	Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity.
28754911	9	44	from	effect	1329:1334	arg1	intestine					1343:1351	the intestine	1339:1351	the intestine	1339:1351	Furthermore, CS significantly increased intestinal Bacteroides acidifaciens population, indirectly exerting its immunomodulatory effect on the intestine.
28754911	6	45	from	Units	948:952	arg1	communities					973:983	fecal microbial communities	957:983	fecal microbial communities in healthy and stressed mice	957:1012	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	8	46	theme	stress-induced	1160:1173	arg1	inflammation					1186:1197	stress-induced intestinal inflammation	1160:1197	stress-induced intestinal inflammation	1160:1197	Together, our findings demonstrated that CS may ameliorate stress-induced intestinal inflammation.
28754911	0	47	dep	structure	47:55	arg1	the					43:45	the	43:45	the	43:45	Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions.
28754911	10	48	theme	biological	1416:1425	arg1	metabolism					1528:1537	carbohydrate metabolism	1515:1537	carbohydrate metabolism	1515:1537	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	10	48	theme	biological	1416:1425	arg1	pathways					1427:1434	biological pathways	1416:1434	biological pathways	1416:1434	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	10	48	theme	biological	1416:1425	arg1	systems					1502:1508	two-component systems	1488:1508	two-component systems	1488:1508	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	10	48	theme	biological	1416:1425	arg1	transporters					1474:1485	ABC transporters	1470:1485	ABC transporters	1470:1485	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	3	49	theme	blood	486:490	arg1	LPS					492:494	blood LPS	486:494	blood LPS	486:494	Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress.
28754911	10	50	theme	ABC	1470:1472	arg1	transporters					1474:1485	ABC transporters	1470:1485	ABC transporters	1470:1485	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	6	51	theme	microbial	861:869	arg1	composition					871:881	gut microbial composition	857:881	gut microbial composition	857:881	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	10	52	theme	carbohydrate	1515:1526	arg1	metabolism					1528:1537	carbohydrate metabolism	1515:1537	carbohydrate metabolism	1515:1537	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	6	53	theme	Taxonomic	938:946	arg1	Units					948:952	Operational Taxonomic Units	926:952	7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice	921:1012	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	6	54	theme	gut	857:859	arg1	composition					871:881	gut microbial composition	857:881	gut microbial composition	857:881	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	1	55	used	used	179:182	arg2	CS					159:160	CS	159:160	CS	159:160	Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity.
28754911	1	55	used	used	179:182	arg2	sulfate					150:156	Chondroitin sulfate	138:156	Chondroitin sulfate (CS)	138:161	Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity.
28754911	2	56	from	composition	379:389	arg1	mice					394:397	mice	394:397	mice	394:397	We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice.
28754911	6	57	dep	%	924:924	arg1	Units					948:952	Operational Taxonomic Units	926:952	7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice	921:1012	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	11	58	theme	CS	1587:1588	arg1	development					1572:1582	the development	1568:1582	the development of CS	1568:1588	Our results will facilitate the development of CS as a bioactive nutraceutical.
28754911	11	58	theme	CS	1587:1588	arg1	nutraceutical					1605:1617	a bioactive nutraceutical	1593:1617	a bioactive nutraceutical	1593:1617	Our results will facilitate the development of CS as a bioactive nutraceutical.
28754911	6	59	theme	stressed	1000:1007	arg1	mice					1009:1012	healthy and stressed mice	988:1012	healthy and stressed mice	988:1012	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	0	60	dep	disaccharides	20:32	arg1	modified					34:41	modified	34:41	disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions	20:135	Chondroitin sulfate disaccharides modified the structure and function of the murine gut microbiome under healthy and stressed conditions.
28754911	1	61	theme	Chondroitin	138:148	arg1	CS					159:160	CS	159:160	CS	159:160	Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity.
28754911	1	61	theme	Chondroitin	138:148	arg1	sulfate					150:156	Chondroitin sulfate	138:156	Chondroitin sulfate (CS)	138:161	Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity.
28754911	10	62	theme	pathways	1427:1434	arg1	metabolism					1528:1537	carbohydrate metabolism	1515:1537	carbohydrate metabolism	1515:1537	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	10	62	theme	pathways	1427:1434	arg1	range					1407:1411	a broad range	1399:1411	a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism	1399:1537	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	10	62	theme	pathways	1427:1434	arg1	transporters					1474:1485	ABC transporters	1470:1485	ABC transporters	1470:1485	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	10	62	theme	pathways	1427:1434	arg1	systems					1502:1508	two-component systems	1488:1508	two-component systems	1488:1508	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	1	63	theme	due	223:225	arg1	purposes					214:221	medical and nutraceutical purposes	188:221	medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity	188:281	Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity.
28754911	4	64	from	level	630:634	arg1	acids					615:619	total fecal short-chain fatty acids	585:619	total fecal short-chain fatty acids from the level significantly repressed in mice under the stress	585:683	CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress.
28754911	5	65	from	increase	773:780	arg1	concentration					800:812	fecal butyrate concentration	785:812	fecal butyrate concentration	785:812	Our findings demonstrated that CS was likely butyrogenic and resulted in a significant increase in fecal butyrate concentration.
28754911	11	66	theme	bioactive	1595:1603	arg1	development					1572:1582	the development	1568:1582	the development of CS	1568:1588	Our results will facilitate the development of CS as a bioactive nutraceutical.
28754911	11	66	theme	bioactive	1595:1603	arg1	nutraceutical					1605:1617	a bioactive nutraceutical	1593:1617	a bioactive nutraceutical	1593:1617	Our results will facilitate the development of CS as a bioactive nutraceutical.
28754911	4	67	theme	short-chain	597:607	arg1	acids					615:619	total fecal short-chain fatty acids	585:619	total fecal short-chain fatty acids from the level significantly repressed in mice under the stress	585:683	CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress.
28754911	6	68	contain	had	832:834	arg2	impact					847:852	a profound impact	836:852	a profound impact	836:852	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	6	68	contain	had	832:834	arg1	disaccharides					818:830	CS disaccharides	815:830	CS disaccharides	815:830	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	6	69	theme	Operational	926:936	arg1	Units					948:952	Operational Taxonomic Units	926:952	7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice	921:1012	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	6	70	theme	healthy	988:994	arg1	mice					1009:1012	healthy and stressed mice	988:1012	healthy and stressed mice	988:1012	CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively.
28754911	10	71	theme	stressed	1442:1449	arg1	condition					1451:1459	stressed condition	1442:1459	stressed condition	1442:1459	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
28754911	10	72	theme	CS	1354:1355	arg1	disaccharides					1357:1369	CS disaccharides	1354:1369	CS disaccharides	1354:1369	CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism.
27450125	0	0	theme	organic	84:90	arg1	acids					92:96	organic acids	84:96	organic acids	84:96	Highly efficient organosolv fractionation of cornstalk into cellulose and lignin in organic acids.
27450125	2	1	theme	lignin	434:439	arg1	products					441:448	crude cellulose and lignin products	414:448	crude cellulose and lignin products of relatively high purity	414:474	It was revealed that mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v) resulted in crude cellulose and lignin products of relatively high purity.
27450125	2	2	from	ratio	379:383	arg1	mixture					331:337	mixture	331:337	mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v)	331:400	It was revealed that mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v) resulted in crude cellulose and lignin products of relatively high purity.
27450125	5	3	theme	acid/water	855:864	arg1	solvents					824:831	mixed solvents	818:831	mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v)	818:879	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	1	4	theme	catalysts	149:157	arg1	effects					114:120	effects	114:120	effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products	114:249	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	5	5	theme	lignin	954:959	arg1	%					973:973	<4%	971:973	<4%	971:973	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	5	5	theme	lignin	954:959	arg1	contents					961:968	very low residual lignin contents	936:968	very low residual lignin contents (<4%)	936:974	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	1	6	theme	products	242:249	arg1	purity					203:208	purity	203:208	purity	203:208	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	1	6	theme	products	242:249	arg1	composition					223:233	chemical composition	214:233	chemical composition	214:233	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	1	6	theme	products	242:249	arg1	yields					195:200	yields	195:200	yields	195:200	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	2	7	theme	purity	469:474	arg1	products					441:448	crude cellulose and lignin products	414:448	crude cellulose and lignin products of relatively high purity	414:474	It was revealed that mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v) resulted in crude cellulose and lignin products of relatively high purity.
27450125	0	8	from	cellulose	60:68	arg1	acids					92:96	organic acids	84:96	organic acids	84:96	Highly efficient organosolv fractionation of cornstalk into cellulose and lignin in organic acids.
27450125	3	9	theme	HCl	488:490	arg1	catalyst					492:499	HCl catalyst	488:499	HCl catalyst	488:499	The use of HCl catalyst contributed to a high crude cellulose yield, while H2SO4 showed an adverse effect on cellulose yield.
27450125	2	10	theme	high	464:467	arg1	purity					469:474	relatively high purity	453:474	relatively high purity	453:474	It was revealed that mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v) resulted in crude cellulose and lignin products of relatively high purity.
27450125	4	11	theme	crude	708:712	arg1	products					724:731	the resulted crude cellulose products	695:731	the resulted crude cellulose products	695:731	However, both of these acidic catalysts contributed to much lower hemicellulose contents in the resulted crude cellulose products compared with those obtained without a catalyst.
27450125	2	12	theme	acid/water	361:370	arg1	mixture					331:337	mixture	331:337	mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v)	331:400	It was revealed that mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v) resulted in crude cellulose and lignin products of relatively high purity.
27450125	5	13	theme	acetic	836:841	arg1	acid/water					855:864	acetic acid/formic acid/water	836:864	acetic acid/formic acid/water (3:6:1, v/v/v)	836:879	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	2	14	theme	cellulose	420:428	arg1	products					441:448	crude cellulose and lignin products	414:448	crude cellulose and lignin products of relatively high purity	414:474	It was revealed that mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v) resulted in crude cellulose and lignin products of relatively high purity.
27450125	1	15	from	effects	114:120	arg1	purity					203:208	purity	203:208	purity	203:208	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	1	15	from	effects	114:120	arg1	composition					223:233	chemical composition	214:233	chemical composition	214:233	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	1	15	from	effects	114:120	arg1	yields					195:200	yields	195:200	yields	195:200	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	4	16	theme	resulted	699:706	arg1	products					724:731	the resulted crude cellulose products	695:731	the resulted crude cellulose products	695:731	However, both of these acidic catalysts contributed to much lower hemicellulose contents in the resulted crude cellulose products compared with those obtained without a catalyst.
27450125	1	17	theme	temperatures	160:171	arg1	effects					114:120	effects	114:120	effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products	114:249	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	0	18	theme	efficient	7:15	arg1	fractionation					28:40	Highly efficient organosolv fractionation	0:40	Highly efficient organosolv fractionation of cornstalk into cellulose and lignin in organic acids.	0:97	Highly efficient organosolv fractionation of cornstalk into cellulose and lignin in organic acids.
27450125	4	19	theme	lower	663:667	arg1	contents					683:690	much lower hemicellulose contents	658:690	much lower hemicellulose contents in the resulted crude cellulose products	658:731	However, both of these acidic catalysts contributed to much lower hemicellulose contents in the resulted crude cellulose products compared with those obtained without a catalyst.
27450125	2	20	theme	acetic	342:347	arg1	acid/water					361:370	acetic acid/formic acid/water	342:370	acetic acid/formic acid/water	342:370	It was revealed that mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v) resulted in crude cellulose and lignin products of relatively high purity.
27450125	1	21	theme	residence	177:185	arg1	time					187:190	residence time	177:190	residence time	177:190	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	3	22	theme	catalyst	492:499	arg1	use					481:483	The use	477:483	The use of HCl catalyst	477:499	The use of HCl catalyst contributed to a high crude cellulose yield, while H2SO4 showed an adverse effect on cellulose yield.
27450125	5	23	theme	low	941:943	arg1	%					973:973	<4%	971:973	<4%	971:973	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	5	23	theme	low	941:943	arg1	contents					961:968	very low residual lignin contents	936:968	very low residual lignin contents (<4%)	936:974	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	1	24	theme	time	187:190	arg1	effects					114:120	effects	114:120	effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products	114:249	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	2	25	theme	3:6:1	388:392	arg1	ratio					379:383	the ratio	375:383	the ratio of 3:6:1 (v/v/v)	375:400	It was revealed that mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v) resulted in crude cellulose and lignin products of relatively high purity.
27450125	4	26	theme	hemicellulose	669:681	arg1	contents					683:690	much lower hemicellulose contents	658:690	much lower hemicellulose contents in the resulted crude cellulose products	658:731	However, both of these acidic catalysts contributed to much lower hemicellulose contents in the resulted crude cellulose products compared with those obtained without a catalyst.
27450125	5	27	theme	mixed	818:822	arg1	solvents					824:831	mixed solvents	818:831	mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v)	818:879	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	0	28	theme	cornstalk	45:53	arg1	fractionation					28:40	Highly efficient organosolv fractionation	0:40	Highly efficient organosolv fractionation of cornstalk into cellulose and lignin in organic acids.	0:97	Highly efficient organosolv fractionation of cornstalk into cellulose and lignin in organic acids.
27450125	1	29	theme	solid/solvent	276:288	arg1	ratio					290:294	the solid/solvent ratio	272:294	the solid/solvent ratio of 1:5 (g/g)	272:307	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	3	30	theme	cellulose	529:537	arg1	yield					539:543	a high crude cellulose yield	516:543	a high crude cellulose yield	516:543	The use of HCl catalyst contributed to a high crude cellulose yield, while H2SO4 showed an adverse effect on cellulose yield.
27450125	3	31	from	effect	576:581	arg1	yield					596:600	cellulose yield	586:600	cellulose yield	586:600	The use of HCl catalyst contributed to a high crude cellulose yield, while H2SO4 showed an adverse effect on cellulose yield.
27450125	2	32	theme	crude	414:418	arg1	products					441:448	crude cellulose and lignin products	414:448	crude cellulose and lignin products of relatively high purity	414:474	It was revealed that mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v) resulted in crude cellulose and lignin products of relatively high purity.
27450125	0	33	from	lignin	74:79	arg1	acids					92:96	organic acids	84:96	organic acids	84:96	Highly efficient organosolv fractionation of cornstalk into cellulose and lignin in organic acids.
27450125	5	34	theme	residual	945:952	arg1	%					973:973	<4%	971:973	<4%	971:973	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	5	34	theme	residual	945:952	arg1	contents					961:968	very low residual lignin contents	936:968	very low residual lignin contents (<4%)	936:974	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	3	35	theme	adverse	568:574	arg1	effect					576:581	an adverse effect	565:581	an adverse effect on cellulose yield	565:600	The use of HCl catalyst contributed to a high crude cellulose yield, while H2SO4 showed an adverse effect on cellulose yield.
27450125	3	36	theme	high	518:521	arg1	yield					539:543	a high crude cellulose yield	516:543	a high crude cellulose yield	516:543	The use of HCl catalyst contributed to a high crude cellulose yield, while H2SO4 showed an adverse effect on cellulose yield.
27450125	5	37	theme	acid/formic	843:853	arg1	acid/water					855:864	acetic acid/formic acid/water	836:864	acetic acid/formic acid/water (3:6:1, v/v/v)	836:879	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	1	38	theme	1:5	299:301	arg1	ratio					290:294	the solid/solvent ratio	272:294	the solid/solvent ratio of 1:5 (g/g)	272:307	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	3	39	theme	crude	523:527	arg1	yield					539:543	a high crude cellulose yield	516:543	a high crude cellulose yield	516:543	The use of HCl catalyst contributed to a high crude cellulose yield, while H2SO4 showed an adverse effect on cellulose yield.
27450125	4	40	theme	acidic	626:631	arg1	catalysts					633:641	these acidic catalysts	620:641	these acidic catalysts	620:641	However, both of these acidic catalysts contributed to much lower hemicellulose contents in the resulted crude cellulose products compared with those obtained without a catalyst.
27450125	4	41	from	contents	683:690	arg1	products					724:731	the resulted crude cellulose products	695:731	the resulted crude cellulose products	695:731	However, both of these acidic catalysts contributed to much lower hemicellulose contents in the resulted crude cellulose products compared with those obtained without a catalyst.
27450125	5	42	theme	crude	915:919	arg1	cellulose					921:929	crude cellulose	915:929	crude cellulose	915:929	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	5	43	dep	acid/water	855:864	arg1	v/v/v					874:878	v/v/v	874:878	v/v/v	874:878	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	5	43	dep	acid/water	855:864	arg1	3:6:1					867:871	3:6:1	867:871	3:6:1	867:871	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	3	44	theme	cellulose	586:594	arg1	yield					596:600	cellulose yield	586:600	cellulose yield	586:600	The use of HCl catalyst contributed to a high crude cellulose yield, while H2SO4 showed an adverse effect on cellulose yield.
27450125	4	45	theme	cellulose	714:722	arg1	products					724:731	the resulted crude cellulose products	695:731	the resulted crude cellulose products	695:731	However, both of these acidic catalysts contributed to much lower hemicellulose contents in the resulted crude cellulose products compared with those obtained without a catalyst.
27450125	1	46	theme	fractionation	125:137	arg1	solvents					139:146	fractionation solvents	125:146	fractionation solvents	125:146	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	2	47	theme	acid/formic	349:359	arg1	acid/water					361:370	acetic acid/formic acid/water	342:370	acetic acid/formic acid/water	342:370	It was revealed that mixture of acetic acid/formic acid/water at the ratio of 3:6:1 (v/v/v) resulted in crude cellulose and lignin products of relatively high purity.
27450125	1	48	theme	chemical	214:221	arg1	composition					223:233	chemical composition	214:233	chemical composition	214:233	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	1	48	theme	chemical	214:221	arg1	yields					195:200	yields	195:200	yields	195:200	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
27450125	0	49	theme	organosolv	17:26	arg1	fractionation					28:40	Highly efficient organosolv fractionation	0:40	Highly efficient organosolv fractionation of cornstalk into cellulose and lignin in organic acids.	0:97	Highly efficient organosolv fractionation of cornstalk into cellulose and lignin in organic acids.
27450125	5	50	from	90°C	799:802	arg1	Fractionation					782:794	Fractionation	782:794	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst	782:904	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	5	50	from	90°C	799:802	arg1	solvents					824:831	mixed solvents	818:831	mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v)	818:879	Fractionation at 90°C for 180min in mixed solvents of acetic acid/formic acid/water (3:6:1, v/v/v) with or without catalyst produced crude cellulose with very low residual lignin contents (<4%).
27450125	1	51	theme	solvents	139:146	arg1	effects					114:120	effects	114:120	effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products	114:249	In this study, effects of fractionation solvents, catalysts, temperatures and residence time on yields, purity and chemical composition of the products were investigated at the solid/solvent ratio of 1:5 (g/g).
26271435	0	0	theme	structural	57:66	arg1	barrier					32:38	water vapor barrier	20:38	water vapor barrier	20:38	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	0	0	theme	structural	57:66	arg1	properties					68:77	mechanical, and structural properties	41:77	mechanical, and structural properties of agar/lignin composite films	41:108	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	9	1	contain	have	1043:1046	arg1	films					1037:1041	agar/lignin films	1025:1041	agar/lignin films	1025:1041	The results suggested that agar/lignin films have a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food.
26271435	9	1	contain	have	1043:1046	arg2	potential					1050:1058	a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food	1048:1187	a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food	1048:1187	The results suggested that agar/lignin films have a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food.
26271435	7	2	theme	lignin	873:878	arg1	content					880:886	lignin content	873:886	lignin content	873:886	The swelling ratio and moisture content of agar/lignin composite films were decreased with increase in lignin content.
26271435	5	3	theme	vapor	640:644	arg1	permeability					646:657	lower water vapor permeability	628:657	lower water vapor permeability compared to the neat agar film	628:688	The agar/lignin films exhibited higher mechanical and UV barrier properties along with lower water vapor permeability compared to the neat agar film.
26271435	5	4	theme	barrier	598:604	arg1	properties					606:615	higher mechanical and UV barrier properties	573:615	properties	606:615	The agar/lignin films exhibited higher mechanical and UV barrier properties along with lower water vapor permeability compared to the neat agar film.
26271435	6	5	theme	SEM	704:706	arg1	results					708:714	The FTIR and SEM results	691:714	The FTIR and SEM results	691:714	The FTIR and SEM results showed the compatibility of lignin with agar polymer.
26271435	7	6	from	increase	861:868	arg1	content					880:886	lignin content	873:886	lignin content	873:886	The swelling ratio and moisture content of agar/lignin composite films were decreased with increase in lignin content.
26271435	4	7	theme	barrier	477:483	arg1	properties					485:494	water vapor barrier, UV light barrier properties	447:494	properties	485:494	In addition, the mechanical, water vapor barrier, UV light barrier properties, FE-SEM, and TGA of the films were analyzed.
26271435	3	8	theme	concentration	328:340	arg1	effect					311:316	The effect	307:316	The effect of lignin concentration (1, 3, 5, and 10wt%) on the performance of the composite films	307:403	The effect of lignin concentration (1, 3, 5, and 10wt%) on the performance of the composite films was studied.
26271435	0	9	from	Effect	0:5	arg1	barrier					32:38	water vapor barrier	20:38	water vapor barrier	20:38	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	0	9	from	Effect	0:5	arg1	properties					68:77	mechanical, and structural properties	41:77	mechanical, and structural properties of agar/lignin composite films	41:108	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	5	10	theme	agar/lignin	545:555	arg1	films					557:561	The agar/lignin films	541:561	The agar/lignin films	541:561	The agar/lignin films exhibited higher mechanical and UV barrier properties along with lower water vapor permeability compared to the neat agar film.
26271435	9	11	theme	barrier	1079:1085	arg1	material					1102:1109	a UV barrier food packaging material	1074:1109	a UV barrier food packaging material	1074:1109	The results suggested that agar/lignin films have a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food.
26271435	8	12	theme	composite	941:949	arg1	films					951:955	agar/lignin composite films	929:955	agar/lignin composite films	929:955	The thermostability and char content of agar/lignin composite films increased with increased lignin content.
26271435	5	13	theme	lower	628:632	arg1	permeability					646:657	lower water vapor permeability	628:657	lower water vapor permeability compared to the neat agar film	628:688	The agar/lignin films exhibited higher mechanical and UV barrier properties along with lower water vapor permeability compared to the neat agar film.
26271435	8	14	theme	agar/lignin	929:939	arg1	films					951:955	agar/lignin composite films	929:955	agar/lignin composite films	929:955	The thermostability and char content of agar/lignin composite films increased with increased lignin content.
26271435	6	15	theme	FTIR	695:698	arg1	results					708:714	The FTIR and SEM results	691:714	The FTIR and SEM results	691:714	The FTIR and SEM results showed the compatibility of lignin with agar polymer.
26271435	0	16	theme	composite	94:102	arg1	films					104:108	agar/lignin composite films	82:108	agar/lignin composite films	82:108	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	7	17	theme	swelling	774:781	arg1	ratio					783:787	The swelling ratio and moisture content	770:808	ratio	783:787	The swelling ratio and moisture content of agar/lignin composite films were decreased with increase in lignin content.
26271435	2	18	theme	reinforcing	253:263	arg1	material					265:272	a reinforcing material	251:272	a reinforcing material	251:272	The lignin was used as a reinforcing material and agar as a biopolymer matrix.
26271435	2	18	theme	reinforcing	253:263	arg1	lignin					232:237	The lignin	228:237	The lignin	228:237	The lignin was used as a reinforcing material and agar as a biopolymer matrix.
26271435	0	19	theme	agar/lignin	82:92	arg1	films					104:108	agar/lignin composite films	82:108	agar/lignin composite films	82:108	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	5	20	theme	neat	675:678	arg1	film					685:688	the neat agar film	671:688	the neat agar film	671:688	The agar/lignin films exhibited higher mechanical and UV barrier properties along with lower water vapor permeability compared to the neat agar film.
26271435	7	21	theme	composite	825:833	arg1	films					835:839	agar/lignin composite films	813:839	agar/lignin composite films	813:839	The swelling ratio and moisture content of agar/lignin composite films were decreased with increase in lignin content.
26271435	2	22	used	used	243:246	arg2	matrix					299:304	a biopolymer matrix	286:304	a biopolymer matrix	286:304	The lignin was used as a reinforcing material and agar as a biopolymer matrix.
26271435	2	22	used	used	243:246	arg2	agar					278:281	agar	278:281	agar	278:281	The lignin was used as a reinforcing material and agar as a biopolymer matrix.
26271435	2	22	used	used	243:246	arg2	lignin					232:237	The lignin	228:237	The lignin	228:237	The lignin was used as a reinforcing material and agar as a biopolymer matrix.
26271435	2	22	used	used	243:246	arg2	material					265:272	a reinforcing material	251:272	a reinforcing material	251:272	The lignin was used as a reinforcing material and agar as a biopolymer matrix.
26271435	1	23	theme	renewable	165:173	arg1	agar					204:207	agar	204:207	agar	204:207	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	1	23	theme	renewable	165:173	arg1	alkali					220:225	lignin alkali	213:225	lignin alkali	213:225	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	1	23	theme	renewable	165:173	arg1	biopolymers					191:201	two renewable resources based biopolymers	161:201	two renewable resources based biopolymers	161:201	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	8	24	theme	increased	972:980	arg1	content					989:995	increased lignin content	972:995	increased lignin content	972:995	The thermostability and char content of agar/lignin composite films increased with increased lignin content.
26271435	7	25	theme	films	835:839	arg1	ratio					783:787	The swelling ratio and moisture content	770:808	ratio	783:787	The swelling ratio and moisture content of agar/lignin composite films were decreased with increase in lignin content.
26271435	7	25	theme	films	835:839	arg1	content					802:808	The swelling ratio and moisture content	770:808	content	802:808	The swelling ratio and moisture content of agar/lignin composite films were decreased with increase in lignin content.
26271435	2	26	theme	biopolymer	288:297	arg1	matrix					299:304	a biopolymer matrix	286:304	a biopolymer matrix	286:304	The lignin was used as a reinforcing material and agar as a biopolymer matrix.
26271435	2	26	theme	biopolymer	288:297	arg1	lignin					232:237	The lignin	228:237	The lignin	228:237	The lignin was used as a reinforcing material and agar as a biopolymer matrix.
26271435	1	27	theme	resources	175:183	arg1	agar					204:207	agar	204:207	agar	204:207	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	1	27	theme	resources	175:183	arg1	alkali					220:225	lignin alkali	213:225	lignin alkali	213:225	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	1	27	theme	resources	175:183	arg1	biopolymers					191:201	two renewable resources based biopolymers	161:201	two renewable resources based biopolymers	161:201	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	8	28	theme	char	913:916	arg1	content					918:924	char content	913:924	char content	913:924	The thermostability and char content of agar/lignin composite films increased with increased lignin content.
26271435	1	29	theme	based	185:189	arg1	agar					204:207	agar	204:207	agar	204:207	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	1	29	theme	based	185:189	arg1	alkali					220:225	lignin alkali	213:225	lignin alkali	213:225	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	1	29	theme	based	185:189	arg1	biopolymers					191:201	two renewable resources based biopolymers	161:201	two renewable resources based biopolymers	161:201	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	4	30	theme	barrier	459:465	arg1	properties					485:494	water vapor barrier, UV light barrier properties	447:494	properties	485:494	In addition, the mechanical, water vapor barrier, UV light barrier properties, FE-SEM, and TGA of the films were analyzed.
26271435	5	31	theme	UV	595:596	arg1	properties					606:615	higher mechanical and UV barrier properties	573:615	properties	606:615	The agar/lignin films exhibited higher mechanical and UV barrier properties along with lower water vapor permeability compared to the neat agar film.
26271435	7	32	theme	agar/lignin	813:823	arg1	films					835:839	agar/lignin composite films	813:839	agar/lignin composite films	813:839	The swelling ratio and moisture content of agar/lignin composite films were decreased with increase in lignin content.
26271435	4	33	theme	films	520:524	arg1	FE-SEM					497:502	FE-SEM	497:502	FE-SEM	497:502	In addition, the mechanical, water vapor barrier, UV light barrier properties, FE-SEM, and TGA of the films were analyzed.
26271435	4	33	theme	films	520:524	arg1	TGA					509:511	TGA	509:511	TGA	509:511	In addition, the mechanical, water vapor barrier, UV light barrier properties, FE-SEM, and TGA of the films were analyzed.
26271435	4	33	theme	films	520:524	arg1	properties					485:494	water vapor barrier, UV light barrier properties	447:494	properties	485:494	In addition, the mechanical, water vapor barrier, UV light barrier properties, FE-SEM, and TGA of the films were analyzed.
26271435	0	34	theme	lignin	10:15	arg1	Effect					0:5	Effect	0:5	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.	0:109	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	4	35	theme	light	471:475	arg1	properties					485:494	water vapor barrier, UV light barrier properties	447:494	properties	485:494	In addition, the mechanical, water vapor barrier, UV light barrier properties, FE-SEM, and TGA of the films were analyzed.
26271435	0	36	theme	films	104:108	arg1	barrier					32:38	water vapor barrier	20:38	water vapor barrier	20:38	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	0	36	theme	films	104:108	arg1	properties					68:77	mechanical, and structural properties	41:77	mechanical, and structural properties of agar/lignin composite films	41:108	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	6	37	theme	agar	756:759	arg1	polymer					761:767	agar polymer	756:767	agar polymer	756:767	The FTIR and SEM results showed the compatibility of lignin with agar polymer.
26271435	8	38	dep	thermostability	893:907	arg1	The					889:891	The	889:891	The	889:891	The thermostability and char content of agar/lignin composite films increased with increased lignin content.
26271435	3	39	theme	composite	389:397	arg1	films					399:403	the composite films	385:403	the composite films	385:403	The effect of lignin concentration (1, 3, 5, and 10wt%) on the performance of the composite films was studied.
26271435	0	40	theme	vapor	26:30	arg1	barrier					32:38	water vapor barrier	20:38	water vapor barrier	20:38	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	0	40	theme	vapor	26:30	arg1	properties					68:77	mechanical, and structural properties	41:77	mechanical, and structural properties of agar/lignin composite films	41:108	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	4	41	theme	UV	468:469	arg1	properties					485:494	water vapor barrier, UV light barrier properties	447:494	properties	485:494	In addition, the mechanical, water vapor barrier, UV light barrier properties, FE-SEM, and TGA of the films were analyzed.
26271435	8	42	theme	lignin	982:987	arg1	content					989:995	increased lignin content	972:995	increased lignin content	972:995	The thermostability and char content of agar/lignin composite films increased with increased lignin content.
26271435	9	43	theme	agar/lignin	1025:1035	arg1	films					1037:1041	agar/lignin films	1025:1041	agar/lignin films	1025:1041	The results suggested that agar/lignin films have a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food.
26271435	0	44	theme	water	20:24	arg1	barrier					32:38	water vapor barrier	20:38	water vapor barrier	20:38	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	0	44	theme	water	20:24	arg1	properties					68:77	mechanical, and structural properties	41:77	mechanical, and structural properties of agar/lignin composite films	41:108	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	5	45	dep	mechanical	580:589	arg1	higher					573:578	higher	573:578	higher	573:578	The agar/lignin films exhibited higher mechanical and UV barrier properties along with lower water vapor permeability compared to the neat agar film.
26271435	5	46	theme	agar	680:683	arg1	film					685:688	the neat agar film	671:688	the neat agar film	671:688	The agar/lignin films exhibited higher mechanical and UV barrier properties along with lower water vapor permeability compared to the neat agar film.
26271435	4	47	dep	properties	485:494	arg1	the					431:433	the	431:433	the	431:433	In addition, the mechanical, water vapor barrier, UV light barrier properties, FE-SEM, and TGA of the films were analyzed.
26271435	3	48	theme	lignin	321:326	arg1	%					360:360	1, 3, 5, and 10wt%	343:360	1, 3, 5, and 10wt%	343:360	The effect of lignin concentration (1, 3, 5, and 10wt%) on the performance of the composite films was studied.
26271435	3	48	theme	lignin	321:326	arg1	concentration					328:340	lignin concentration	321:340	lignin concentration (1, 3, 5, and 10wt%)	321:361	The effect of lignin concentration (1, 3, 5, and 10wt%) on the performance of the composite films was studied.
26271435	4	49	theme	mechanical	435:444	arg1	properties					485:494	water vapor barrier, UV light barrier properties	447:494	properties	485:494	In addition, the mechanical, water vapor barrier, UV light barrier properties, FE-SEM, and TGA of the films were analyzed.
26271435	4	50	theme	vapor	453:457	arg1	properties					485:494	water vapor barrier, UV light barrier properties	447:494	properties	485:494	In addition, the mechanical, water vapor barrier, UV light barrier properties, FE-SEM, and TGA of the films were analyzed.
26271435	8	51	theme	films	951:955	arg1	thermostability					893:907	thermostability	893:907	thermostability	893:907	The thermostability and char content of agar/lignin composite films increased with increased lignin content.
26271435	8	51	theme	films	951:955	arg1	content					918:924	char content	913:924	char content	913:924	The thermostability and char content of agar/lignin composite films increased with increased lignin content.
26271435	9	52	theme	food	1184:1187	arg1	shelf-life					1157:1166	the shelf-life	1153:1166	the shelf-life of the packaged food	1153:1187	The results suggested that agar/lignin films have a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food.
26271435	6	53	theme	lignin	744:749	arg1	compatibility					727:739	the compatibility	723:739	the compatibility of lignin	723:749	The FTIR and SEM results showed the compatibility of lignin with agar polymer.
26271435	7	54	theme	moisture	793:800	arg1	content					802:808	The swelling ratio and moisture content	770:808	content	802:808	The swelling ratio and moisture content of agar/lignin composite films were decreased with increase in lignin content.
26271435	4	55	theme	water	447:451	arg1	properties					485:494	water vapor barrier, UV light barrier properties	447:494	properties	485:494	In addition, the mechanical, water vapor barrier, UV light barrier properties, FE-SEM, and TGA of the films were analyzed.
26271435	9	56	theme	packaging	1092:1100	arg1	material					1102:1109	a UV barrier food packaging material	1074:1109	a UV barrier food packaging material	1074:1109	The results suggested that agar/lignin films have a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food.
26271435	9	57	theme	UV	1076:1077	arg1	material					1102:1109	a UV barrier food packaging material	1074:1109	a UV barrier food packaging material	1074:1109	The results suggested that agar/lignin films have a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food.
26271435	1	58	theme	Biodegradable	111:123	arg1	films					135:139	Biodegradable composite films	111:139	Biodegradable composite films	111:139	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	1	59	theme	lignin	213:218	arg1	alkali					220:225	lignin alkali	213:225	lignin alkali	213:225	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	1	59	theme	lignin	213:218	arg1	biopolymers					191:201	two renewable resources based biopolymers	161:201	two renewable resources based biopolymers	161:201	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	1	60	theme	composite	125:133	arg1	films					135:139	Biodegradable composite films	111:139	Biodegradable composite films	111:139	Biodegradable composite films were prepared using two renewable resources based biopolymers, agar and lignin alkali.
26271435	9	61	theme	food	1087:1090	arg1	material					1102:1109	a UV barrier food packaging material	1074:1109	a UV barrier food packaging material	1074:1109	The results suggested that agar/lignin films have a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food.
26271435	3	62	theme	films	399:403	arg1	performance					370:380	the performance	366:380	the performance of the composite films	366:403	The effect of lignin concentration (1, 3, 5, and 10wt%) on the performance of the composite films was studied.
26271435	3	63	from	effect	311:316	arg1	performance					370:380	the performance	366:380	the performance of the composite films	366:403	The effect of lignin concentration (1, 3, 5, and 10wt%) on the performance of the composite films was studied.
26271435	9	64	theme	food	1127:1130	arg1	safety					1132:1137	food safety	1127:1137	food safety	1127:1137	The results suggested that agar/lignin films have a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food.
26271435	0	65	theme	mechanical	41:50	arg1	barrier					32:38	water vapor barrier	20:38	water vapor barrier	20:38	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	0	65	theme	mechanical	41:50	arg1	properties					68:77	mechanical, and structural properties	41:77	mechanical, and structural properties of agar/lignin composite films	41:108	Effect of lignin on water vapor barrier, mechanical, and structural properties of agar/lignin composite films.
26271435	9	66	theme	packaged	1175:1182	arg1	food					1184:1187	the packaged food	1171:1187	the packaged food	1171:1187	The results suggested that agar/lignin films have a potential to be used as a UV barrier food packaging material for maintaining food safety and extending the shelf-life of the packaged food.
26271435	5	67	theme	water	634:638	arg1	permeability					646:657	lower water vapor permeability	628:657	lower water vapor permeability compared to the neat agar film	628:688	The agar/lignin films exhibited higher mechanical and UV barrier properties along with lower water vapor permeability compared to the neat agar film.
26343574	5	0	theme	continuous	808:817	arg1	reactors					829:836	continuous anaerobic reactors	808:836	continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process	808:906	Prospective research should evaluate enzymatic pretreatments in continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process.
26343574	2	1	theme	microalgae	360:369	arg1	composition					381:391	the microalgae cell wall composition	356:391	the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein)	356:443	Cellulase, glucohydrolase and an enzyme mix composed of cellulase, glucohydrolase and xylanase were selected based on the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein).
26343574	5	2	theme	economic	879:886	arg1	cost					888:891	economic cost	879:891	economic cost	879:891	Prospective research should evaluate enzymatic pretreatments in continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process.
26343574	3	3	theme	pretreatment	541:552	arg1	6h					535:536	6h	535:536	6h of pretreatment with an enzyme dose of 1% for cellulase and the enzyme mix	535:611	All of them increased organic matter solubilisation, obtaining high values already after 6h of pretreatment with an enzyme dose of 1% for cellulase and the enzyme mix.
26343574	5	4	dep	balance	867:873	arg1	the					856:858	the	856:858	the	856:858	Prospective research should evaluate enzymatic pretreatments in continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process.
26343574	4	5	theme	enzyme	718:723	arg1	mix					725:727	the enzyme mix	714:727	the enzyme mix	714:727	BMP tests with pretreated microalgae showed a methane yield increase of 8 and 15% for cellulase and the enzyme mix, respectively.
26343574	3	6	theme	high	509:512	arg1	values					514:519	high values	509:519	high values	509:519	All of them increased organic matter solubilisation, obtaining high values already after 6h of pretreatment with an enzyme dose of 1% for cellulase and the enzyme mix.
26343574	5	7	theme	process	900:906	arg1	balance					867:873	energy balance	860:873	energy balance	860:873	Prospective research should evaluate enzymatic pretreatments in continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process.
26343574	5	7	theme	process	900:906	arg1	cost					888:891	economic cost	879:891	economic cost	879:891	Prospective research should evaluate enzymatic pretreatments in continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process.
26343574	5	8	theme	anaerobic	819:827	arg1	reactors					829:836	continuous anaerobic reactors	808:836	continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process	808:906	Prospective research should evaluate enzymatic pretreatments in continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process.
26343574	4	9	with	tests	618:622	arg1	microalgae					640:649	pretreated microalgae	629:649	pretreated microalgae	629:649	BMP tests with pretreated microalgae showed a methane yield increase of 8 and 15% for cellulase and the enzyme mix, respectively.
26343574	4	10	theme	pretreated	629:638	arg1	microalgae					640:649	pretreated microalgae	629:649	pretreated microalgae	629:649	BMP tests with pretreated microalgae showed a methane yield increase of 8 and 15% for cellulase and the enzyme mix, respectively.
26343574	0	11	theme	biogas	10:15	arg1	production					17:26	biogas production	10:26	biogas production from microalgae	10:42	Improving biogas production from microalgae by enzymatic pretreatment.
26343574	0	12	from	microalgae	33:42	arg1	production					17:26	biogas production	10:26	biogas production from microalgae	10:42	Improving biogas production from microalgae by enzymatic pretreatment.
26343574	1	13	theme	biochemical	195:205	arg1	tests					231:235	biochemical methane potential (BMP) tests	195:235	biochemical methane potential (BMP) tests	195:235	In this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests.
26343574	1	13	theme	biochemical	195:205	arg1	BMP					226:228	BMP	226:228	BMP	226:228	In this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests.
26343574	5	14	theme	enzymatic	781:789	arg1	pretreatments					791:803	enzymatic pretreatments	781:803	enzymatic pretreatments	781:803	Prospective research should evaluate enzymatic pretreatments in continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process.
26343574	1	15	theme	enzymatic	86:94	arg1	pretreatment					96:107	enzymatic pretreatment	86:107	enzymatic pretreatment of microalgal biomass	86:129	In this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests.
26343574	1	16	theme	methane	207:213	arg1	tests					231:235	biochemical methane potential (BMP) tests	195:235	biochemical methane potential (BMP) tests	195:235	In this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests.
26343574	1	16	theme	methane	207:213	arg1	BMP					226:228	BMP	226:228	BMP	226:228	In this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests.
26343574	1	17	theme	potential	215:223	arg1	tests					231:235	biochemical methane potential (BMP) tests	195:235	biochemical methane potential (BMP) tests	195:235	In this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests.
26343574	1	17	theme	potential	215:223	arg1	BMP					226:228	BMP	226:228	BMP	226:228	In this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests.
26343574	3	18	theme	enzyme	562:567	arg1	dose					569:572	an enzyme dose	559:572	an enzyme dose of 1% for cellulase and the enzyme mix	559:611	All of them increased organic matter solubilisation, obtaining high values already after 6h of pretreatment with an enzyme dose of 1% for cellulase and the enzyme mix.
26343574	3	19	theme	organic	468:474	arg1	solubilisation					483:496	organic matter solubilisation	468:496	organic matter solubilisation	468:496	All of them increased organic matter solubilisation, obtaining high values already after 6h of pretreatment with an enzyme dose of 1% for cellulase and the enzyme mix.
26343574	4	20	theme	yield	668:672	arg1	increase					674:681	a methane yield increase	658:681	a methane yield increase of 8 and 15% for cellulase	658:708	BMP tests with pretreated microalgae showed a methane yield increase of 8 and 15% for cellulase and the enzyme mix, respectively.
26343574	5	21	theme	Prospective	744:754	arg1	research					756:763	Prospective research	744:763	Prospective research	744:763	Prospective research should evaluate enzymatic pretreatments in continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process.
26343574	3	22	with	pretreatment	541:552	arg1	dose					569:572	an enzyme dose	559:572	an enzyme dose of 1% for cellulase and the enzyme mix	559:611	All of them increased organic matter solubilisation, obtaining high values already after 6h of pretreatment with an enzyme dose of 1% for cellulase and the enzyme mix.
26343574	5	23	theme	energy	860:865	arg1	balance					867:873	energy balance	860:873	energy balance	860:873	Prospective research should evaluate enzymatic pretreatments in continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process.
26343574	1	24	theme	microalgal	112:121	arg1	biomass					123:129	microalgal biomass	112:129	microalgal biomass	112:129	In this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests.
26343574	0	25	theme	enzymatic	47:55	arg1	pretreatment					57:68	enzymatic pretreatment	47:68	enzymatic pretreatment	47:68	Improving biogas production from microalgae by enzymatic pretreatment.
26343574	3	26	theme	%	578:578	arg1	dose					569:572	an enzyme dose	559:572	an enzyme dose of 1% for cellulase and the enzyme mix	559:611	All of them increased organic matter solubilisation, obtaining high values already after 6h of pretreatment with an enzyme dose of 1% for cellulase and the enzyme mix.
26343574	1	27	theme	biomass	123:129	arg1	pretreatment					96:107	enzymatic pretreatment	86:107	enzymatic pretreatment of microalgal biomass	86:129	In this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests.
26343574	2	28	theme	enzyme	271:276	arg1	mix					278:280	an enzyme mix	268:280	an enzyme mix composed of cellulase, glucohydrolase and xylanase	268:331	Cellulase, glucohydrolase and an enzyme mix composed of cellulase, glucohydrolase and xylanase were selected based on the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein).
26343574	3	29	theme	enzyme	602:607	arg1	mix					609:611	the enzyme mix	598:611	the enzyme mix	598:611	All of them increased organic matter solubilisation, obtaining high values already after 6h of pretreatment with an enzyme dose of 1% for cellulase and the enzyme mix.
26343574	4	30	theme	methane	660:666	arg1	increase					674:681	a methane yield increase	658:681	a methane yield increase of 8 and 15% for cellulase	658:708	BMP tests with pretreated microalgae showed a methane yield increase of 8 and 15% for cellulase and the enzyme mix, respectively.
26343574	3	31	theme	matter	476:481	arg1	solubilisation					483:496	organic matter solubilisation	468:496	organic matter solubilisation	468:496	All of them increased organic matter solubilisation, obtaining high values already after 6h of pretreatment with an enzyme dose of 1% for cellulase and the enzyme mix.
26343574	2	32	dep	composition	381:391	arg1	cellulose					394:402	cellulose	394:402	cellulose	394:402	Cellulase, glucohydrolase and an enzyme mix composed of cellulase, glucohydrolase and xylanase were selected based on the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein).
26343574	2	32	dep	composition	381:391	arg1	glycoprotein					431:442	glycoprotein	431:442	glycoprotein	431:442	Cellulase, glucohydrolase and an enzyme mix composed of cellulase, glucohydrolase and xylanase were selected based on the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein).
26343574	2	32	dep	composition	381:391	arg1	pectin					420:425	pectin	420:425	pectin	420:425	Cellulase, glucohydrolase and an enzyme mix composed of cellulase, glucohydrolase and xylanase were selected based on the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein).
26343574	2	32	dep	composition	381:391	arg1	hemicellulose					405:417	hemicellulose	405:417	hemicellulose	405:417	Cellulase, glucohydrolase and an enzyme mix composed of cellulase, glucohydrolase and xylanase were selected based on the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein).
26343574	1	33	theme	different	154:162	arg1	conditions					164:173	different conditions	154:173	different conditions	154:173	In this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests.
26343574	4	34	theme	%	694:694	arg1	increase					674:681	a methane yield increase	658:681	a methane yield increase of 8 and 15% for cellulase	658:708	BMP tests with pretreated microalgae showed a methane yield increase of 8 and 15% for cellulase and the enzyme mix, respectively.
26343574	4	34	theme	%	694:694	arg1	mix					725:727	the enzyme mix	714:727	the enzyme mix	714:727	BMP tests with pretreated microalgae showed a methane yield increase of 8 and 15% for cellulase and the enzyme mix, respectively.
26343574	2	35	gly	glycoprotein	431:442	arg1	glycoprotein					431:442	glycoprotein	431:442	glycoprotein	431:442	Cellulase, glucohydrolase and an enzyme mix composed of cellulase, glucohydrolase and xylanase were selected based on the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein).
26343574	4	36	theme	BMP	614:616	arg1	tests					618:622	BMP tests	614:622	BMP tests with pretreated microalgae	614:649	BMP tests with pretreated microalgae showed a methane yield increase of 8 and 15% for cellulase and the enzyme mix, respectively.
26343574	2	37	theme	wall	376:379	arg1	composition					381:391	the microalgae cell wall composition	356:391	the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein)	356:443	Cellulase, glucohydrolase and an enzyme mix composed of cellulase, glucohydrolase and xylanase were selected based on the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein).
26343574	2	38	theme	cell	371:374	arg1	composition					381:391	the microalgae cell wall composition	356:391	the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein)	356:443	Cellulase, glucohydrolase and an enzyme mix composed of cellulase, glucohydrolase and xylanase were selected based on the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein).
26531259	5	0	theme	good	1017:1020	arg1	response					1022:1029	good response	1017:1029	good response towards the target (prion protein)	1017:1064	The developed aptasensor showed good response towards the target (prion protein) with a low detection limit of 160 fM.
26531259	1	1	theme	host-guest	161:170	arg1	strategy					172:179	A novel competitive host-guest strategy	141:179	A novel competitive host-guest strategy regulated by protein biogate	141:208	A novel competitive host-guest strategy regulated by protein biogate was developed for sensitive and selective analysis of prion protein.
26531259	1	2	theme	prion	264:268	arg1	protein					270:276	prion protein	264:276	prion protein	264:276	A novel competitive host-guest strategy regulated by protein biogate was developed for sensitive and selective analysis of prion protein.
26531259	4	3	theme	peak	916:919	arg1	current					921:927	the oxidation peak current	902:927	the oxidation peak current of MB (IMB)	902:939	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	1	4	theme	protein	270:276	arg1	analysis					252:259	sensitive and selective analysis	228:259	sensitive and selective analysis of prion protein	228:276	A novel competitive host-guest strategy regulated by protein biogate was developed for sensitive and selective analysis of prion protein.
26531259	0	5	theme	ratiometric	97:107	arg1	assay					125:129	sensitive ratiometric electrochemical assay	87:129	sensitive ratiometric electrochemical assay of prion	87:138	Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion.
26531259	3	6	theme	large	665:669	arg1	peak					681:684	a large oxidation peak	663:684	a large oxidation peak of FCA	663:691	In the absence of prion, MB-Apt could be displaced by ferrocenecarboxylic acid (FCA) due to its stronger binding affinity to β-CD, resulting in a large oxidation peak of FCA.
26531259	4	7	theme	protein	791:797	arg1	biogate					799:805	protein biogate	791:805	protein biogate	791:805	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	4	8	theme	prion	722:726	arg1	presence					710:717	the presence	706:717	the presence of prion	706:726	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	5	9	theme	160 fM	1096:1101	arg1	limit					1087:1091	a low detection limit	1071:1091	a low detection limit of 160 fM	1071:1101	The developed aptasensor showed good response towards the target (prion protein) with a low detection limit of 160 fM.
26531259	0	10	theme	sensitive	87:95	arg1	assay					125:129	sensitive ratiometric electrochemical assay	87:129	sensitive ratiometric electrochemical assay of prion	87:138	Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion.
26531259	5	11	theme	developed	989:997	arg1	aptasensor					999:1008	The developed aptasensor	985:1008	The developed aptasensor	985:1008	The developed aptasensor showed good response towards the target (prion protein) with a low detection limit of 160 fM.
26531259	4	12	theme	β-CD	829:832	arg1	cavity					819:824	seal the cavity	810:824	seal the cavity	810:824	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	6	13	theme	extensive	1236:1244	arg1	applications					1246:1257	extensive applications	1236:1257	extensive applications in bioanalysis	1236:1272	By changing the specific aptamers, this strategy could be easily extended to detect other proteins, showing promising potential for extensive applications in bioanalysis.
26531259	2	14	theme	blue	293:296	arg1	MB-Apt					325:330	MB-Apt	325:330	MB-Apt	325:330	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	2	14	theme	blue	293:296	arg1	aptamer					316:322	The methylene blue (MB)-tagged prion aptamer	279:322	The methylene blue (MB)-tagged prion aptamer (MB-Apt)	279:331	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	1	15	theme	protein	194:200	arg1	biogate					202:208	protein biogate	194:208	protein biogate	194:208	A novel competitive host-guest strategy regulated by protein biogate was developed for sensitive and selective analysis of prion protein.
26531259	2	16	theme	MWCNTs-β-CD	401:411	arg1	electrode					453:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	0	17	theme	electrochemical	109:123	arg1	assay					125:129	sensitive ratiometric electrochemical assay	87:129	sensitive ratiometric electrochemical assay of prion	87:138	Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion.
26531259	4	18	theme	biogate	799:805	arg1	formation					778:786	the formation	774:786	the formation of protein biogate	774:805	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	3	19	theme	prion	537:541	arg1	absence					526:532	the absence	522:532	the absence of prion	522:541	In the absence of prion, MB-Apt could be displaced by ferrocenecarboxylic acid (FCA) due to its stronger binding affinity to β-CD, resulting in a large oxidation peak of FCA.
26531259	3	20	theme	FCA	689:691	arg1	peak					681:684	a large oxidation peak	663:684	a large oxidation peak of FCA	663:691	In the absence of prion, MB-Apt could be displaced by ferrocenecarboxylic acid (FCA) due to its stronger binding affinity to β-CD, resulting in a large oxidation peak of FCA.
26531259	4	21	theme	oxidation	906:914	arg1	current					921:927	the oxidation peak current	902:927	the oxidation peak current of MB (IMB)	902:939	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	6	22	theme	other	1188:1192	arg1	proteins					1194:1201	other proteins	1188:1201	other proteins	1188:1201	By changing the specific aptamers, this strategy could be easily extended to detect other proteins, showing promising potential for extensive applications in bioanalysis.
26531259	2	23	theme	nanotubes-β-cyclodextrins	374:398	arg1	electrode					453:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	0	24	theme	Smart	0:4	arg1	protein					6:12	Smart protein	0:12	Smart protein	0:12	Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion.
26531259	4	25	theme	seal	810:813	arg1	cavity					819:824	seal the cavity	810:824	seal the cavity	810:824	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	2	26	theme	prion	310:314	arg1	MB-Apt					325:330	MB-Apt	325:330	MB-Apt	325:330	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	2	26	theme	prion	310:314	arg1	aptamer					316:322	The methylene blue (MB)-tagged prion aptamer	279:322	The methylene blue (MB)-tagged prion aptamer (MB-Apt)	279:331	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	6	27	theme	promising	1212:1220	arg1	potential					1222:1230	promising potential	1212:1230	promising potential for extensive applications in bioanalysis	1212:1272	By changing the specific aptamers, this strategy could be easily extended to detect other proteins, showing promising potential for extensive applications in bioanalysis.
26531259	2	28	theme	carbon	367:372	arg1	electrode					453:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	2	29	theme	GC	449:450	arg1	electrode					453:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	0	30	theme	prion	134:138	arg1	assay					125:129	sensitive ratiometric electrochemical assay	87:129	sensitive ratiometric electrochemical assay of prion	87:138	Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion.
26531259	2	31	theme	-tagged	302:308	arg1	MB-Apt					325:330	MB-Apt	325:330	MB-Apt	325:330	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	2	31	theme	-tagged	302:308	arg1	aptamer					316:322	The methylene blue (MB)-tagged prion aptamer	279:322	The methylene blue (MB)-tagged prion aptamer (MB-Apt)	279:331	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	6	32	from	applications	1246:1257	arg1	bioanalysis					1262:1272	bioanalysis	1262:1272	bioanalysis	1262:1272	By changing the specific aptamers, this strategy could be easily extended to detect other proteins, showing promising potential for extensive applications in bioanalysis.
26531259	2	33	theme	carbon	441:446	arg1	electrode					453:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	5	34	theme	low	1073:1075	arg1	limit					1087:1091	a low detection limit	1071:1091	a low detection limit of 160 fM	1071:1101	The developed aptasensor showed good response towards the target (prion protein) with a low detection limit of 160 fM.
26531259	2	35	theme	multiwalled	355:365	arg1	electrode					453:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	2	36	theme	glassy	434:439	arg1	electrode					453:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	4	37	theme	MB	932:933	arg1	current					921:927	the oxidation peak current	902:927	the oxidation peak current of MB (IMB)	902:939	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	5	38	theme	detection	1077:1085	arg1	limit					1087:1091	a low detection limit	1071:1091	a low detection limit of 160 fM	1071:1101	The developed aptasensor showed good response towards the target (prion protein) with a low detection limit of 160 fM.
26531259	6	39	theme	specific	1120:1127	arg1	aptamers					1129:1136	the specific aptamers	1116:1136	the specific aptamers	1116:1136	By changing the specific aptamers, this strategy could be easily extended to detect other proteins, showing promising potential for extensive applications in bioanalysis.
26531259	2	40	theme	composites-modified	414:432	arg1	electrode					453:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode	351:461	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	2	41	theme	host-guest	475:484	arg1	interaction					486:496	the host-guest interaction	471:496	the host-guest interaction between β-CD and MB	471:516	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	4	42	theme	MB	876:877	arg1	displacement					860:871	the guest displacement	850:871	the guest displacement of MB by FCA	850:884	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	3	43	theme	stronger	615:622	arg1	affinity					632:639	its stronger binding affinity	611:639	its stronger binding affinity to β-CD	611:647	In the absence of prion, MB-Apt could be displaced by ferrocenecarboxylic acid (FCA) due to its stronger binding affinity to β-CD, resulting in a large oxidation peak of FCA.
26531259	1	44	theme	sensitive	228:236	arg1	analysis					252:259	sensitive and selective analysis	228:259	sensitive and selective analysis of prion protein	228:276	A novel competitive host-guest strategy regulated by protein biogate was developed for sensitive and selective analysis of prion protein.
26531259	5	45	theme	prion	1051:1055	arg1	protein					1057:1063	the target (prion protein)	1039:1064	the target (prion protein)	1039:1064	The developed aptasensor showed good response towards the target (prion protein) with a low detection limit of 160 fM.
26531259	3	46	theme	binding	624:630	arg1	affinity					632:639	its stronger binding affinity	611:639	its stronger binding affinity to β-CD	611:647	In the absence of prion, MB-Apt could be displaced by ferrocenecarboxylic acid (FCA) due to its stronger binding affinity to β-CD, resulting in a large oxidation peak of FCA.
26531259	3	47	theme	ferrocenecarboxylic	573:591	arg1	FCA					599:601	FCA	599:601	FCA	599:601	In the absence of prion, MB-Apt could be displaced by ferrocenecarboxylic acid (FCA) due to its stronger binding affinity to β-CD, resulting in a large oxidation peak of FCA.
26531259	3	47	theme	ferrocenecarboxylic	573:591	arg1	acid					593:596	ferrocenecarboxylic acid	573:596	ferrocenecarboxylic acid (FCA)	573:602	In the absence of prion, MB-Apt could be displaced by ferrocenecarboxylic acid (FCA) due to its stronger binding affinity to β-CD, resulting in a large oxidation peak of FCA.
26531259	5	48	theme	target	1043:1048	arg1	protein					1057:1063	the target (prion protein)	1039:1064	the target (prion protein)	1039:1064	The developed aptasensor showed good response towards the target (prion protein) with a low detection limit of 160 fM.
26531259	2	49	dep	blue	293:296	arg1	MB					299:300	MB	299:300	MB	299:300	The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB.
26531259	4	50	theme	guest	854:858	arg1	displacement					860:871	the guest displacement	850:871	the guest displacement of MB by FCA	850:884	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	4	51	theme	prion-aptamer	742:754	arg1	interaction					756:766	the specific prion-aptamer interaction	729:766	the specific prion-aptamer interaction	729:766	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	4	52	theme	specific	733:740	arg1	interaction					756:766	the specific prion-aptamer interaction	729:766	the specific prion-aptamer interaction	729:766	However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased.
26531259	1	53	theme	selective	242:250	arg1	analysis					252:259	sensitive and selective analysis	228:259	sensitive and selective analysis of prion protein	228:276	A novel competitive host-guest strategy regulated by protein biogate was developed for sensitive and selective analysis of prion protein.
26531259	0	54	theme	host-guest	60:69	arg1	interaction					71:81	competitive host-guest interaction	48:81	competitive host-guest interaction	48:81	Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion.
26531259	1	55	theme	novel	143:147	arg1	strategy					172:179	A novel competitive host-guest strategy	141:179	A novel competitive host-guest strategy regulated by protein biogate	141:208	A novel competitive host-guest strategy regulated by protein biogate was developed for sensitive and selective analysis of prion protein.
26531259	0	56	theme	competitive	48:58	arg1	interaction					71:81	competitive host-guest interaction	48:81	competitive host-guest interaction	48:81	Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion.
26531259	3	57	theme	oxidation	671:679	arg1	peak					681:684	a large oxidation peak	663:684	a large oxidation peak of FCA	663:691	In the absence of prion, MB-Apt could be displaced by ferrocenecarboxylic acid (FCA) due to its stronger binding affinity to β-CD, resulting in a large oxidation peak of FCA.
26531259	1	58	theme	competitive	149:159	arg1	strategy					172:179	A novel competitive host-guest strategy	141:179	A novel competitive host-guest strategy regulated by protein biogate	141:208	A novel competitive host-guest strategy regulated by protein biogate was developed for sensitive and selective analysis of prion protein.
28365287	7	0	theme	control	1209:1215	arg1	film					1217:1220	gelatin control film	1201:1220	gelatin control film	1201:1220	Moreover, the calorimetric results were comparable to those of gelatin control film with Tg value 45°C and increased crystallinity percentage with increasing incorporation of microparticles.
28365287	4	1	theme	Composite	526:534	arg1	films					536:540	Composite films	526:540	Composite films	526:540	Composite films were prepared by incorporating chitosan-coated microparticles, at various concentrations (0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)) in the gelatin-based films.
28365287	6	2	theme	scanning	979:986	arg1	microscopes					997:1007	scanning electron microscopes	979:1007	scanning electron microscopes	979:1007	The microstructure studies done by scanning electron microscopes, revealed different micropores embedded with oil resulting from the incorporation of the microparticles into the gelatin matrix.
28365287	4	3	theme	powder	690:695	arg1	weight					668:673	the weight	664:673	the weight of the gelatin powder	664:695	Composite films were prepared by incorporating chitosan-coated microparticles, at various concentrations (0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)) in the gelatin-based films.
28365287	7	4	theme	calorimetric	1152:1163	arg1	results					1165:1171	the calorimetric results	1148:1171	the calorimetric results	1148:1171	Moreover, the calorimetric results were comparable to those of gelatin control film with Tg value 45°C and increased crystallinity percentage with increasing incorporation of microparticles.
28365287	7	4	theme	calorimetric	1152:1163	arg1	comparable					1178:1187	comparable	1178:1187	comparable	1178:1187	Moreover, the calorimetric results were comparable to those of gelatin control film with Tg value 45°C and increased crystallinity percentage with increasing incorporation of microparticles.
28365287	8	5	theme	active	1442:1447	arg1	compounds					1449:1457	liposoluble active compounds	1430:1457	liposoluble active compounds	1430:1457	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	8	6	theme	liposoluble	1430:1440	arg1	compounds					1449:1457	liposoluble active compounds	1430:1457	liposoluble active compounds	1430:1457	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	1	7	dep	development	129:139	arg1	film					151:154	an active film	141:154	development an active film based on gelatin incorporated with antioxidant, rutin carried into microparticles	129:236	The aim of this study was development an active film based on gelatin incorporated with antioxidant, rutin carried into microparticles.
28365287	3	8	theme	average	400:406	arg1	size					408:411	an average size	397:411	an average size of 520±4nm	397:422	The generated microparticles had an average size of 520±4nm and a span of 0.3 were formulated by a rotor-stator homogenize at the homogenization speed 10,000rpm.
28365287	8	9	theme	composite	1354:1362	arg1	films					1378:1382	composite biodegradable films	1354:1382	composite biodegradable films	1354:1382	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	5	10	theme	chitosan	903:910	arg1	concentration					912:924	chitosan concentration	903:924	chitosan concentration higher than 0.5%	903:941	For the prepared films, the results showed that obtained physicochemical, water vapor barrier, and mechanical were compared with native gelatin film with a slight decrease for chitosan concentration higher than 0.5%.
28365287	5	11	theme	slight	883:888	arg1	decrease					890:897	a slight decrease	881:897	a slight decrease for chitosan concentration higher than 0.5%	881:941	For the prepared films, the results showed that obtained physicochemical, water vapor barrier, and mechanical were compared with native gelatin film with a slight decrease for chitosan concentration higher than 0.5%.
28365287	0	12	with	charged	83:89	arg1	rutin					96:100	rutin	96:100	rutin	96:100	Properties of gelatin-based films incorporated with chitosan-coated microparticles charged with rutin.
28365287	6	13	theme	embedded	1040:1047	arg1	micropores					1029:1038	different micropores	1019:1038	different micropores embedded with oil resulting from the incorporation of the microparticles into the gelatin matrix	1019:1135	The microstructure studies done by scanning electron microscopes, revealed different micropores embedded with oil resulting from the incorporation of the microparticles into the gelatin matrix.
28365287	8	14	theme	films	1378:1382	arg1	alternative					1403:1413	a good alternative	1396:1413	a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties	1396:1508	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	8	14	theme	films	1378:1382	arg1	concept					1343:1349	This original concept	1329:1349	This original concept of composite biodegradable films	1329:1382	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	8	15	with	packaging	1479:1487	arg1	properties					1499:1508	good properties	1494:1508	good properties	1494:1508	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	3	16	theme	520±4nm	416:422	arg1	span					430:433	a span	428:433	a span of 0.3	428:440	The generated microparticles had an average size of 520±4nm and a span of 0.3 were formulated by a rotor-stator homogenize at the homogenization speed 10,000rpm.
28365287	3	16	theme	520±4nm	416:422	arg1	size					408:411	an average size	397:411	an average size of 520±4nm	397:422	The generated microparticles had an average size of 520±4nm and a span of 0.3 were formulated by a rotor-stator homogenize at the homogenization speed 10,000rpm.
28365287	2	17	theme	chitosan-coated	332:346	arg1	microparticles					348:361	the chitosan-coated microparticles	328:361	the chitosan-coated microparticles	328:361	The complexation between oppositely charged lecithin and chitosan was applied to prepare the chitosan-coated microparticles.
28365287	6	18	theme	microstructure	948:961	arg1	studies					963:969	The microstructure studies	944:969	The microstructure studies	944:969	The microstructure studies done by scanning electron microscopes, revealed different micropores embedded with oil resulting from the incorporation of the microparticles into the gelatin matrix.
28365287	3	19	from	10,000rpm	515:523	arg1	homogenize					476:485	a rotor-stator homogenize	461:485	a rotor-stator homogenize at the homogenization speed 10,000rpm	461:523	The generated microparticles had an average size of 520±4nm and a span of 0.3 were formulated by a rotor-stator homogenize at the homogenization speed 10,000rpm.
28365287	5	20	theme	higher	926:931	arg1	concentration					912:924	chitosan concentration	903:924	chitosan concentration higher than 0.5%	903:941	For the prepared films, the results showed that obtained physicochemical, water vapor barrier, and mechanical were compared with native gelatin film with a slight decrease for chitosan concentration higher than 0.5%.
28365287	5	21	theme	native	856:861	arg1	film					871:874	native gelatin film	856:874	native gelatin film	856:874	For the prepared films, the results showed that obtained physicochemical, water vapor barrier, and mechanical were compared with native gelatin film with a slight decrease for chitosan concentration higher than 0.5%.
28365287	0	22	theme	films	28:32	arg1	Properties					0:9	Properties	0:9	Properties of gelatin-based films	0:32	Properties of gelatin-based films incorporated with chitosan-coated microparticles charged with rutin.
28365287	8	23	theme	good	1398:1401	arg1	alternative					1403:1413	a good alternative	1396:1413	a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties	1396:1508	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	8	23	theme	good	1398:1401	arg1	concept					1343:1349	This original concept	1329:1349	This original concept of composite biodegradable films	1329:1382	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	7	24	theme	microparticles	1313:1326	arg1	incorporation					1296:1308	incorporation	1296:1308	incorporation of microparticles	1296:1326	Moreover, the calorimetric results were comparable to those of gelatin control film with Tg value 45°C and increased crystallinity percentage with increasing incorporation of microparticles.
28365287	0	25	theme	gelatin-based	14:26	arg1	films					28:32	gelatin-based films	14:32	gelatin-based films	14:32	Properties of gelatin-based films incorporated with chitosan-coated microparticles charged with rutin.
28365287	8	26	theme	active	1472:1477	arg1	packaging					1479:1487	an active packaging	1469:1487	an active packaging with good properties	1469:1508	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	7	27	with	comparable	1178:1187	arg1	value					1230:1234	Tg value 45°C	1227:1239	Tg value 45°C	1227:1239	Moreover, the calorimetric results were comparable to those of gelatin control film with Tg value 45°C and increased crystallinity percentage with increasing incorporation of microparticles.
28365287	1	28	theme	active	144:149	arg1	film					151:154	an active film	141:154	development an active film based on gelatin incorporated with antioxidant, rutin carried into microparticles	129:236	The aim of this study was development an active film based on gelatin incorporated with antioxidant, rutin carried into microparticles.
28365287	4	29	theme	various	608:614	arg1	%					652:652	0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)	632:696	0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)	632:696	Composite films were prepared by incorporating chitosan-coated microparticles, at various concentrations (0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)) in the gelatin-based films.
28365287	4	29	theme	various	608:614	arg1	concentrations					616:629	various concentrations	608:629	various concentrations (0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)) in the gelatin-based films	608:724	Composite films were prepared by incorporating chitosan-coated microparticles, at various concentrations (0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)) in the gelatin-based films.
28365287	8	30	theme	original	1334:1341	arg1	alternative					1403:1413	a good alternative	1396:1413	a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties	1396:1508	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	8	30	theme	original	1334:1341	arg1	concept					1343:1349	This original concept	1329:1349	This original concept of composite biodegradable films	1329:1382	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	3	31	theme	rotor-stator	463:474	arg1	homogenize					476:485	a rotor-stator homogenize	461:485	a rotor-stator homogenize at the homogenization speed 10,000rpm	461:523	The generated microparticles had an average size of 520±4nm and a span of 0.3 were formulated by a rotor-stator homogenize at the homogenization speed 10,000rpm.
28365287	3	32	theme	0.3	438:440	arg1	span					430:433	a span	428:433	a span of 0.3	428:440	The generated microparticles had an average size of 520±4nm and a span of 0.3 were formulated by a rotor-stator homogenize at the homogenization speed 10,000rpm.
28365287	3	32	theme	0.3	438:440	arg1	size					408:411	an average size	397:411	an average size of 520±4nm	397:422	The generated microparticles had an average size of 520±4nm and a span of 0.3 were formulated by a rotor-stator homogenize at the homogenization speed 10,000rpm.
28365287	5	33	theme	water	801:805	arg1	barrier					813:819	water vapor barrier	801:819	water vapor barrier	801:819	For the prepared films, the results showed that obtained physicochemical, water vapor barrier, and mechanical were compared with native gelatin film with a slight decrease for chitosan concentration higher than 0.5%.
28365287	6	34	theme	different	1019:1027	arg1	micropores					1029:1038	different micropores	1019:1038	different micropores embedded with oil resulting from the incorporation of the microparticles into the gelatin matrix	1019:1135	The microstructure studies done by scanning electron microscopes, revealed different micropores embedded with oil resulting from the incorporation of the microparticles into the gelatin matrix.
28365287	5	35	theme	gelatin	863:869	arg1	film					871:874	native gelatin film	856:874	native gelatin film	856:874	For the prepared films, the results showed that obtained physicochemical, water vapor barrier, and mechanical were compared with native gelatin film with a slight decrease for chitosan concentration higher than 0.5%.
28365287	8	36	theme	biodegradable	1364:1376	arg1	films					1378:1382	composite biodegradable films	1354:1382	composite biodegradable films	1354:1382	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	0	37	theme	charged	83:89	arg1	microparticles					68:81	chitosan-coated microparticles	52:81	chitosan-coated microparticles charged with rutin	52:100	Properties of gelatin-based films incorporated with chitosan-coated microparticles charged with rutin.
28365287	4	38	dep	%	652:652	arg1	based					655:659	based	655:659	based on the weight of the gelatin powder	655:695	Composite films were prepared by incorporating chitosan-coated microparticles, at various concentrations (0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)) in the gelatin-based films.
28365287	6	39	theme	microparticles	1098:1111	arg1	incorporation					1077:1089	the incorporation	1073:1089	the incorporation of the microparticles into the gelatin matrix	1073:1135	The microstructure studies done by scanning electron microscopes, revealed different micropores embedded with oil resulting from the incorporation of the microparticles into the gelatin matrix.
28365287	1	40	theme	study	119:123	arg1	development					129:139	development	129:139	development an active film based on gelatin incorporated with antioxidant, rutin carried into microparticles	129:236	The aim of this study was development an active film based on gelatin incorporated with antioxidant, rutin carried into microparticles.
28365287	1	40	theme	study	119:123	arg1	aim					107:109	The aim	103:109	The aim of this study	103:123	The aim of this study was development an active film based on gelatin incorporated with antioxidant, rutin carried into microparticles.
28365287	0	41	theme	chitosan-coated	52:66	arg1	microparticles					68:81	chitosan-coated microparticles	52:81	chitosan-coated microparticles charged with rutin	52:100	Properties of gelatin-based films incorporated with chitosan-coated microparticles charged with rutin.
28365287	7	42	theme	gelatin	1201:1207	arg1	film					1217:1220	gelatin control film	1201:1220	gelatin control film	1201:1220	Moreover, the calorimetric results were comparable to those of gelatin control film with Tg value 45°C and increased crystallinity percentage with increasing incorporation of microparticles.
28365287	6	43	theme	gelatin	1122:1128	arg1	matrix					1130:1135	the gelatin matrix	1118:1135	the gelatin matrix	1118:1135	The microstructure studies done by scanning electron microscopes, revealed different micropores embedded with oil resulting from the incorporation of the microparticles into the gelatin matrix.
28365287	4	44	theme	chitosan-coated	573:587	arg1	microparticles					589:602	chitosan-coated microparticles	573:602	chitosan-coated microparticles	573:602	Composite films were prepared by incorporating chitosan-coated microparticles, at various concentrations (0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)) in the gelatin-based films.
28365287	8	45	theme	good	1494:1497	arg1	properties					1499:1508	good properties	1494:1508	good properties	1494:1508	This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.
28365287	3	46	theme	homogenization	494:507	arg1	10,000rpm					515:523	the homogenization speed 10,000rpm	490:523	the homogenization speed 10,000rpm	490:523	The generated microparticles had an average size of 520±4nm and a span of 0.3 were formulated by a rotor-stator homogenize at the homogenization speed 10,000rpm.
28365287	2	47	theme	charged	275:281	arg1	lecithin					283:290	lecithin	283:290	lecithin	283:290	The complexation between oppositely charged lecithin and chitosan was applied to prepare the chitosan-coated microparticles.
28365287	3	48	theme	speed	509:513	arg1	10,000rpm					515:523	the homogenization speed 10,000rpm	490:523	the homogenization speed 10,000rpm	490:523	The generated microparticles had an average size of 520±4nm and a span of 0.3 were formulated by a rotor-stator homogenize at the homogenization speed 10,000rpm.
28365287	4	49	theme	gelatin-based	706:718	arg1	films					720:724	the gelatin-based films	702:724	the gelatin-based films	702:724	Composite films were prepared by incorporating chitosan-coated microparticles, at various concentrations (0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)) in the gelatin-based films.
28365287	5	50	theme	prepared	735:742	arg1	films					744:748	the prepared films	731:748	the prepared films	731:748	For the prepared films, the results showed that obtained physicochemical, water vapor barrier, and mechanical were compared with native gelatin film with a slight decrease for chitosan concentration higher than 0.5%.
28365287	6	51	with	embedded	1040:1047	arg1	oil					1054:1056	oil	1054:1056	oil resulting from the incorporation of the microparticles into the gelatin matrix	1054:1135	The microstructure studies done by scanning electron microscopes, revealed different micropores embedded with oil resulting from the incorporation of the microparticles into the gelatin matrix.
28365287	4	52	theme	gelatin	682:688	arg1	powder					690:695	the gelatin powder	678:695	the gelatin powder	678:695	Composite films were prepared by incorporating chitosan-coated microparticles, at various concentrations (0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)) in the gelatin-based films.
28365287	7	53	theme	Tg	1227:1228	arg1	value					1230:1234	Tg value 45°C	1227:1239	Tg value 45°C	1227:1239	Moreover, the calorimetric results were comparable to those of gelatin control film with Tg value 45°C and increased crystallinity percentage with increasing incorporation of microparticles.
28365287	3	54	theme	generated	368:376	arg1	microparticles					378:391	The generated microparticles	364:391	The generated microparticles had an average size of 520±4nm and a span of 0.3	364:440	The generated microparticles had an average size of 520±4nm and a span of 0.3 were formulated by a rotor-stator homogenize at the homogenization speed 10,000rpm.
28365287	5	55	theme	vapor	807:811	arg1	barrier					813:819	water vapor barrier	801:819	water vapor barrier	801:819	For the prepared films, the results showed that obtained physicochemical, water vapor barrier, and mechanical were compared with native gelatin film with a slight decrease for chitosan concentration higher than 0.5%.
28365287	7	56	theme	crystallinity	1255:1267	arg1	percentage					1269:1278	crystallinity percentage	1255:1278	crystallinity percentage	1255:1278	Moreover, the calorimetric results were comparable to those of gelatin control film with Tg value 45°C and increased crystallinity percentage with increasing incorporation of microparticles.
28365287	4	57	from	concentrations	616:629	arg1	films					720:724	the gelatin-based films	702:724	the gelatin-based films	702:724	Composite films were prepared by incorporating chitosan-coated microparticles, at various concentrations (0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)) in the gelatin-based films.
28365287	6	58	theme	electron	988:995	arg1	microscopes					997:1007	scanning electron microscopes	979:1007	scanning electron microscopes	979:1007	The microstructure studies done by scanning electron microscopes, revealed different micropores embedded with oil resulting from the incorporation of the microparticles into the gelatin matrix.
25046733	3	0	theme	antibiotic	676:685	arg1	poly					694:697	antibiotic loaded poly	676:697	antibiotic loaded poly(lactic-co-glycolic acid) (PLGA)	676:729	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	0	theme	antibiotic	676:685	arg1	acid					718:721	lactic-co-glycolic acid	699:721	lactic-co-glycolic acid	699:721	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	0	theme	antibiotic	676:685	arg1	PLGA					725:728	PLGA	725:728	PLGA	725:728	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	6	1	theme	3:2	1208:1210	arg1	PPF					1212:1214	3:2 PPF	1208:1214	3:2 PPF	1208:1214	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	3	2	theme	propylene	536:544	arg1	poly					531:534	poly	531:534	poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen,	531:670	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	2	theme	propylene	536:544	arg1	fumarate					546:553	propylene fumarate	536:553	propylene fumarate	536:553	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	3	with	poly	694:697	arg1	pyrrolidone					575:585	N-vinyl pyrrolidone	567:585	N-vinyl pyrrolidone (NVP)	567:591	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	3	with	poly	694:697	arg1	NVP					588:590	NVP	588:590	NVP	588:590	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	4	4	theme	PLGA	860:863	arg1	microparticles					865:878	PLGA microparticles	860:878	PLGA microparticles	860:878	CMC was incorporated at 40 wt % to impart rapid porosity while PLGA microparticles were incorporated at 30 or 40 wt % to release either clindamycin or colistin.
25046733	1	5	theme	tissue	261:266	arg1	healing					268:274	soft tissue healing	256:274	soft tissue healing over the defect	256:290	Space maintainers (SMs) used for craniofacial reconstruction function to preserve the void space created upon bone loss and promote soft tissue healing over the defect.
25046733	3	6	theme	lactic-co-glycolic	699:716	arg1	poly					694:697	antibiotic loaded poly	676:697	antibiotic loaded poly(lactic-co-glycolic acid) (PLGA)	676:729	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	6	theme	lactic-co-glycolic	699:716	arg1	acid					718:721	lactic-co-glycolic acid	699:721	lactic-co-glycolic acid	699:721	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	6	7	theme	%	1338:1338	arg1	content					1345:1351	30 and 40 wt % PLGA content	1325:1351	30 and 40 wt % PLGA content	1325:1351	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	3	8	with	poly	531:534	arg1	pyrrolidone					575:585	N-vinyl pyrrolidone	567:585	N-vinyl pyrrolidone (NVP)	567:591	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	8	with	poly	531:534	arg1	NVP					588:590	NVP	588:590	NVP	588:590	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	7	9	theme	degradation	1526:1536	arg1	conditions					1538:1547	accelerated degradation conditions	1514:1547	accelerated degradation conditions	1514:1547	In formulations with 3:2 PPF:NVP ratio, incorporation of 40 versus 30 wt % PLGA significantly increased the porosity at 8 weeks under accelerated degradation conditions.
25046733	6	10	theme	%	1237:1237	arg1	%					1255:1255	63.2 ± 0.8%	1245:1255	63.2 ± 0.8%	1245:1255	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	6	10	theme	%	1237:1237	arg1	PLGA					1239:1242	30 wt % PLGA	1231:1242	30 wt % PLGA (63.2 ± 0.8%)	1231:1256	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	7	11	theme	NVP	1409:1411	arg1	ratio					1413:1417	3:2 PPF:NVP ratio	1401:1417	3:2 PPF:NVP ratio	1401:1417	In formulations with 3:2 PPF:NVP ratio, incorporation of 40 versus 30 wt % PLGA significantly increased the porosity at 8 weeks under accelerated degradation conditions.
25046733	4	12	theme	wt	910:911	arg1	%					913:913	30 or 40 wt %	901:913	30 or 40 wt %	901:913	CMC was incorporated at 40 wt % to impart rapid porosity while PLGA microparticles were incorporated at 30 or 40 wt % to release either clindamycin or colistin.
25046733	6	13	theme	1.0	1300:1302	arg1	±					1298:1298	±	1298:1298	±	1298:1298	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	6	14	theme	Mass	1149:1152	arg1	loss					1154:1157	Mass loss	1149:1157	Mass loss	1149:1157	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	5	15	theme	PLGA	1023:1026	arg1	%					1031:1031	PLGA wt %	1023:1031	PLGA wt %	1023:1031	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	3	16	theme	crosslinking	600:611	arg1	hydrogel					649:656	carboxymethylcellulose (CMC) hydrogel	620:656	carboxymethylcellulose (CMC) hydrogel as a porogen	620:669	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	16	theme	crosslinking	600:611	arg1	agent					613:617	the crosslinking agent	596:617	the crosslinking agent	596:617	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	7	17	theme	PPF	1405:1407	arg1	ratio					1413:1417	3:2 PPF:NVP ratio	1401:1417	3:2 PPF:NVP ratio	1401:1417	In formulations with 3:2 PPF:NVP ratio, incorporation of 40 versus 30 wt % PLGA significantly increased the porosity at 8 weeks under accelerated degradation conditions.
25046733	3	18	theme	antibiotic	749:758	arg1	microparticles					731:744	poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles	531:744	poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles	531:744	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	18	theme	antibiotic	749:758	arg1	carriers					760:767	antibiotic carriers	749:767	antibiotic carriers	749:767	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	1	19	theme	Space	124:128	arg1	maintainers					130:140	Space maintainers	124:140	Space maintainers (SMs) used for craniofacial reconstruction	124:183	Space maintainers (SMs) used for craniofacial reconstruction function to preserve the void space created upon bone loss and promote soft tissue healing over the defect.
25046733	1	19	theme	Space	124:128	arg1	SMs					143:145	SMs	143:145	SMs	143:145	Space maintainers (SMs) used for craniofacial reconstruction function to preserve the void space created upon bone loss and promote soft tissue healing over the defect.
25046733	9	20	theme	constructs	1771:1780	arg1	composition					1743:1753	the composition	1739:1753	the composition of PPF/CMC/PLGA constructs	1739:1780	This study demonstrates that the composition of PPF/CMC/PLGA constructs can be modulated to achieve properties suitable for craniofacial degradable space maintenance applications.
25046733	5	21	theme	drug	1042:1045	arg1	dose					1047:1050	the drug dose	1038:1050	the drug dose	1038:1050	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	5	21	theme	drug	1042:1045	arg1	effects					997:1003	the effects	993:1003	the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct	993:1146	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	7	22	with	formulations	1383:1394	arg1	ratio					1413:1417	3:2 PPF:NVP ratio	1401:1417	3:2 PPF:NVP ratio	1401:1417	In formulations with 3:2 PPF:NVP ratio, incorporation of 40 versus 30 wt % PLGA significantly increased the porosity at 8 weeks under accelerated degradation conditions.
25046733	0	23	theme	craniofacial	79:90	arg1	applications					110:121	craniofacial space maintenance applications	79:121	craniofacial space maintenance applications	79:121	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.
25046733	7	24	theme	%	1453:1453	arg1	PLGA					1455:1458	40 versus 30 wt % PLGA	1437:1458	40 versus 30 wt % PLGA	1437:1458	In formulations with 3:2 PPF:NVP ratio, incorporation of 40 versus 30 wt % PLGA significantly increased the porosity at 8 weeks under accelerated degradation conditions.
25046733	2	25	theme	potential	413:421	arg1	contamination					433:445	potential bacterial contamination	413:445	potential bacterial contamination during implantation	413:465	Polymethylmethacrylate-based SMs present several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation.
25046733	0	26	theme	maintenance	98:108	arg1	applications					110:121	craniofacial space maintenance applications	79:121	craniofacial space maintenance applications	79:121	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.
25046733	8	27	theme	minimum	1622:1628	arg1	concentration					1641:1653	the minimum inhibitory concentration	1618:1653	the minimum inhibitory concentration for target pathogens for 45 and 77 days	1618:1693	Constructs released clindamycin or colistin at concentrations above the minimum inhibitory concentration for target pathogens for 45 and 77 days, respectively.
25046733	9	28	theme	degradable	1847:1856	arg1	applications					1876:1887	craniofacial degradable space maintenance applications	1834:1887	craniofacial degradable space maintenance applications	1834:1887	This study demonstrates that the composition of PPF/CMC/PLGA constructs can be modulated to achieve properties suitable for craniofacial degradable space maintenance applications.
25046733	0	29	theme	Degradable	0:9	arg1	constructs					64:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs	0:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.	0:122	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.
25046733	5	30	theme	porosity	1079:1086	arg1	change					1088:1093	temporal porosity change	1070:1093	temporal porosity change	1070:1093	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	9	31	theme	maintenance	1864:1874	arg1	applications					1876:1887	craniofacial degradable space maintenance applications	1834:1887	craniofacial degradable space maintenance applications	1834:1887	This study demonstrates that the composition of PPF/CMC/PLGA constructs can be modulated to achieve properties suitable for craniofacial degradable space maintenance applications.
25046733	6	32	theme	NVP	1282:1284	arg1	ratio					1286:1290	NVP ratio	1282:1290	constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio	1186:1290	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	0	33	theme	poly	33:36	arg1	constructs					64:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs	0:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.	0:122	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.
25046733	5	34	theme	mass	1059:1062	arg1	loss					1064:1067	mass loss	1059:1067	mass loss	1059:1067	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	2	35	theme	implant	362:368	arg1	exposure					370:377	implant exposure	362:377	implant exposure	362:377	Polymethylmethacrylate-based SMs present several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation.
25046733	0	36	theme	propylene	38:46	arg1	constructs					64:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs	0:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.	0:122	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.
25046733	3	37	theme	composite	501:509	arg1	material					511:518	a novel composite material	493:518	a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen	493:779	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	5	38	theme	composite	1128:1136	arg1	construct					1138:1146	the composite construct	1124:1146	the composite construct	1124:1146	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	2	39	theme	removal	390:396	arg1	surgeries					398:406	secondary removal surgeries	380:406	secondary removal surgeries	380:406	Polymethylmethacrylate-based SMs present several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation.
25046733	1	40	theme	bone	234:237	arg1	loss					239:242	bone loss	234:242	bone loss	234:242	Space maintainers (SMs) used for craniofacial reconstruction function to preserve the void space created upon bone loss and promote soft tissue healing over the defect.
25046733	6	41	theme	2:3	1274:1276	arg1	PPF					1278:1280	the 2:3 PPF	1270:1280	the 2:3 PPF	1270:1280	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	0	42	dep	Degradable	0:9	arg1	antibiotic					12:21	antibiotic	12:21	antibiotic	12:21	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.
25046733	8	43	theme	target	1659:1664	arg1	pathogens					1666:1674	target pathogens	1659:1674	target pathogens	1659:1674	Constructs released clindamycin or colistin at concentrations above the minimum inhibitory concentration for target pathogens for 45 and 77 days, respectively.
25046733	5	44	theme	release	1104:1110	arg1	kinetics					1112:1119	the mass loss, temporal porosity change and drug release kinetics	1055:1119	the mass loss, temporal porosity change and drug release kinetics of the composite construct	1055:1146	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	4	45	theme	rapid	839:843	arg1	porosity					845:852	rapid porosity	839:852	rapid porosity	839:852	CMC was incorporated at 40 wt % to impart rapid porosity while PLGA microparticles were incorporated at 30 or 40 wt % to release either clindamycin or colistin.
25046733	3	46	theme	loaded	687:692	arg1	poly					694:697	antibiotic loaded poly	676:697	antibiotic loaded poly(lactic-co-glycolic acid) (PLGA)	676:729	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	46	theme	loaded	687:692	arg1	acid					718:721	lactic-co-glycolic acid	699:721	lactic-co-glycolic acid	699:721	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	46	theme	loaded	687:692	arg1	PLGA					725:728	PLGA	725:728	PLGA	725:728	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	1	47	theme	soft	256:259	arg1	healing					268:274	soft tissue healing	256:274	soft tissue healing over the defect	256:290	Space maintainers (SMs) used for craniofacial reconstruction function to preserve the void space created upon bone loss and promote soft tissue healing over the defect.
25046733	6	48	dep	constructs	1186:1195	arg1	ratio					1220:1224	NVP ratio	1216:1224	constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio	1186:1290	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	6	48	dep	constructs	1186:1195	arg1	ratio					1286:1290	NVP ratio	1282:1290	constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio	1186:1290	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	6	49	theme	PLGA	1340:1343	arg1	content					1345:1351	30 and 40 wt % PLGA content	1325:1351	30 and 40 wt % PLGA content	1325:1351	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	1	50	theme	craniofacial	157:168	arg1	reconstruction					170:183	craniofacial reconstruction	157:183	craniofacial reconstruction	157:183	Space maintainers (SMs) used for craniofacial reconstruction function to preserve the void space created upon bone loss and promote soft tissue healing over the defect.
25046733	4	51	theme	wt	824:825	arg1	%					827:827	40 wt %	821:827	40 wt %	821:827	CMC was incorporated at 40 wt % to impart rapid porosity while PLGA microparticles were incorporated at 30 or 40 wt % to release either clindamycin or colistin.
25046733	5	52	dep	effects	997:1003	arg1	dose					1047:1050	the drug dose	1038:1050	the drug dose	1038:1050	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	5	52	dep	effects	997:1003	arg1	%					1031:1031	PLGA wt %	1023:1031	PLGA wt %	1023:1031	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	5	52	dep	effects	997:1003	arg1	ratio					1016:1020	NVP ratio	1012:1020	NVP ratio	1012:1020	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	5	52	dep	effects	997:1003	arg1	effects					997:1003	the effects	993:1003	the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct	993:1146	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	6	53	theme	wt	1335:1336	arg1	content					1345:1351	30 and 40 wt % PLGA content	1325:1351	30 and 40 wt % PLGA content	1325:1351	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	7	54	theme	accelerated	1514:1524	arg1	conditions					1538:1547	accelerated degradation conditions	1514:1547	accelerated degradation conditions	1514:1547	In formulations with 3:2 PPF:NVP ratio, incorporation of 40 versus 30 wt % PLGA significantly increased the porosity at 8 weeks under accelerated degradation conditions.
25046733	2	55	theme	Polymethylmethacrylate-based	293:320	arg1	SMs					322:324	Polymethylmethacrylate-based SMs	293:324	Polymethylmethacrylate-based SMs	293:324	Polymethylmethacrylate-based SMs present several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation.
25046733	5	56	from	effects	997:1003	arg1	kinetics					1112:1119	the mass loss, temporal porosity change and drug release kinetics	1055:1119	the mass loss, temporal porosity change and drug release kinetics of the composite construct	1055:1146	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	6	57	theme	wt	1234:1235	arg1	%					1255:1255	63.2 ± 0.8%	1245:1255	63.2 ± 0.8%	1245:1255	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	6	57	theme	wt	1234:1235	arg1	PLGA					1239:1242	30 wt % PLGA	1231:1242	30 wt % PLGA (63.2 ± 0.8%)	1231:1256	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	6	58	with	ratio	1220:1224	arg1	%					1255:1255	63.2 ± 0.8%	1245:1255	63.2 ± 0.8%	1245:1255	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	6	58	with	ratio	1220:1224	arg1	PLGA					1239:1242	30 wt % PLGA	1231:1242	30 wt % PLGA (63.2 ± 0.8%)	1231:1256	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	1	59	theme	void	210:213	arg1	space					215:219	the void space	206:219	the void space created upon bone loss	206:242	Space maintainers (SMs) used for craniofacial reconstruction function to preserve the void space created upon bone loss and promote soft tissue healing over the defect.
25046733	7	60	theme	3:2	1401:1403	arg1	ratio					1413:1417	3:2 PPF:NVP ratio	1401:1417	3:2 PPF:NVP ratio	1401:1417	In formulations with 3:2 PPF:NVP ratio, incorporation of 40 versus 30 wt % PLGA significantly increased the porosity at 8 weeks under accelerated degradation conditions.
25046733	7	61	theme	PLGA	1455:1458	arg1	incorporation					1420:1432	incorporation	1420:1432	incorporation of 40 versus 30 wt % PLGA	1420:1458	In formulations with 3:2 PPF:NVP ratio, incorporation of 40 versus 30 wt % PLGA significantly increased the porosity at 8 weeks under accelerated degradation conditions.
25046733	5	62	theme	wt	1028:1029	arg1	%					1031:1031	PLGA wt %	1023:1031	PLGA wt %	1023:1031	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	9	63	theme	PPF/CMC/PLGA	1758:1769	arg1	constructs					1771:1780	PPF/CMC/PLGA constructs	1758:1780	PPF/CMC/PLGA constructs	1758:1780	This study demonstrates that the composition of PPF/CMC/PLGA constructs can be modulated to achieve properties suitable for craniofacial degradable space maintenance applications.
25046733	6	64	theme	NVP	1216:1218	arg1	ratio					1220:1224	NVP ratio	1216:1224	constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio	1186:1290	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	5	65	theme	PPF	1008:1010	arg1	dose					1047:1050	the drug dose	1038:1050	the drug dose	1038:1050	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	5	65	theme	PPF	1008:1010	arg1	ratio					1016:1020	NVP ratio	1012:1020	NVP ratio	1012:1020	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	5	65	theme	PPF	1008:1010	arg1	effects					997:1003	the effects	993:1003	the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct	993:1146	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	3	66	theme	carboxymethylcellulose	620:641	arg1	hydrogel					649:656	carboxymethylcellulose (CMC) hydrogel	620:656	carboxymethylcellulose (CMC) hydrogel as a porogen	620:669	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	66	theme	carboxymethylcellulose	620:641	arg1	agent					613:617	the crosslinking agent	596:617	the crosslinking agent	596:617	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	67	theme	N-vinyl	567:573	arg1	pyrrolidone					575:585	N-vinyl pyrrolidone	567:585	N-vinyl pyrrolidone (NVP)	567:591	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	67	theme	N-vinyl	567:573	arg1	NVP					588:590	NVP	588:590	NVP	588:590	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	0	68	theme	-based	57:62	arg1	constructs					64:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs	0:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.	0:122	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.
25046733	5	69	theme	NVP	1012:1014	arg1	ratio					1016:1020	NVP ratio	1012:1020	NVP ratio	1012:1020	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	5	69	theme	NVP	1012:1014	arg1	effects					997:1003	the effects	993:1003	the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct	993:1146	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	6	70	theme	±	1250:1250	arg1	%					1255:1255	63.2 ± 0.8%	1245:1255	63.2 ± 0.8%	1245:1255	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	6	70	theme	±	1250:1250	arg1	PLGA					1239:1242	30 wt % PLGA	1231:1242	30 wt % PLGA (63.2 ± 0.8%)	1231:1256	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	0	71	theme	space	92:96	arg1	applications					110:121	craniofacial space maintenance applications	79:121	craniofacial space maintenance applications	79:121	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.
25046733	9	72	theme	suitable	1821:1828	arg1	properties					1810:1819	properties	1810:1819	properties suitable for craniofacial degradable space maintenance applications	1810:1887	This study demonstrates that the composition of PPF/CMC/PLGA constructs can be modulated to achieve properties suitable for craniofacial degradable space maintenance applications.
25046733	6	73	contain	containing	1197:1206	arg2	PPF					1212:1214	3:2 PPF	1208:1214	3:2 PPF	1208:1214	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	6	73	contain	containing	1197:1206	arg1	constructs					1186:1195	constructs	1186:1195	constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio	1186:1290	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	9	74	theme	craniofacial	1834:1845	arg1	applications					1876:1887	craniofacial degradable space maintenance applications	1834:1887	craniofacial degradable space maintenance applications	1834:1887	This study demonstrates that the composition of PPF/CMC/PLGA constructs can be modulated to achieve properties suitable for craniofacial degradable space maintenance applications.
25046733	6	75	theme	80.3	1293:1296	arg1	±					1298:1298	±	1298:1298	±	1298:1298	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	7	76	theme	wt	1450:1451	arg1	PLGA					1455:1458	40 versus 30 wt % PLGA	1437:1458	40 versus 30 wt % PLGA	1437:1458	In formulations with 3:2 PPF:NVP ratio, incorporation of 40 versus 30 wt % PLGA significantly increased the porosity at 8 weeks under accelerated degradation conditions.
25046733	9	77	theme	space	1858:1862	arg1	applications					1876:1887	craniofacial degradable space maintenance applications	1834:1887	craniofacial degradable space maintenance applications	1834:1887	This study demonstrates that the composition of PPF/CMC/PLGA constructs can be modulated to achieve properties suitable for craniofacial degradable space maintenance applications.
25046733	0	78	theme	releasing	23:31	arg1	constructs					64:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs	0:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.	0:122	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.
25046733	5	79	theme	change	1088:1093	arg1	kinetics					1112:1119	the mass loss, temporal porosity change and drug release kinetics	1055:1119	the mass loss, temporal porosity change and drug release kinetics of the composite construct	1055:1146	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	8	80	theme	inhibitory	1630:1639	arg1	concentration					1641:1653	the minimum inhibitory concentration	1618:1653	the minimum inhibitory concentration for target pathogens for 45 and 77 days	1618:1693	Constructs released clindamycin or colistin at concentrations above the minimum inhibitory concentration for target pathogens for 45 and 77 days, respectively.
25046733	3	81	theme	novel	495:499	arg1	material					511:518	a novel composite material	493:518	a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen	493:779	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	5	82	theme	drug	1099:1102	arg1	release					1104:1110	drug release	1099:1110	drug release	1099:1110	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	3	83	theme	CMC	644:646	arg1	hydrogel					649:656	carboxymethylcellulose (CMC) hydrogel	620:656	carboxymethylcellulose (CMC) hydrogel as a porogen	620:669	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	83	theme	CMC	644:646	arg1	agent					613:617	the crosslinking agent	596:617	the crosslinking agent	596:617	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	2	84	theme	bacterial	423:431	arg1	contamination					433:445	potential bacterial contamination	413:445	potential bacterial contamination during implantation	413:465	Polymethylmethacrylate-based SMs present several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation.
25046733	0	85	theme	fumarate	48:55	arg1	constructs					64:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs	0:73	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.	0:122	Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications.
25046733	5	86	theme	loss	1064:1067	arg1	kinetics					1112:1119	the mass loss, temporal porosity change and drug release kinetics	1055:1119	the mass loss, temporal porosity change and drug release kinetics of the composite construct	1055:1146	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	3	87	theme	poly	531:534	arg1	microparticles					731:744	poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles	531:744	poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles	531:744	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	87	theme	poly	531:534	arg1	carriers					760:767	antibiotic carriers	749:767	antibiotic carriers	749:767	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	5	88	theme	temporal	1070:1077	arg1	change					1088:1093	temporal porosity change	1070:1093	temporal porosity change	1070:1093	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	2	89	theme	several	334:340	arg1	contamination					433:445	potential bacterial contamination	413:445	potential bacterial contamination during implantation	413:465	Polymethylmethacrylate-based SMs present several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation.
25046733	2	89	theme	several	334:340	arg1	surgeries					398:406	secondary removal surgeries	380:406	secondary removal surgeries	380:406	Polymethylmethacrylate-based SMs present several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation.
25046733	2	89	theme	several	334:340	arg1	drawbacks					342:350	several drawbacks	334:350	several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation	334:465	Polymethylmethacrylate-based SMs present several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation.
25046733	2	89	theme	several	334:340	arg1	exposure					370:377	implant exposure	362:377	implant exposure	362:377	Polymethylmethacrylate-based SMs present several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation.
25046733	6	90	theme	±	1314:1314	arg1	%					1319:1319	85.3 ± 1.3%	1309:1319	85.3 ± 1.3% for 30 and 40 wt % PLGA content	1309:1351	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	5	91	theme	construct	1138:1146	arg1	kinetics					1112:1119	the mass loss, temporal porosity change and drug release kinetics	1055:1119	the mass loss, temporal porosity change and drug release kinetics of the composite construct	1055:1146	This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct.
25046733	6	92	dep	decreased	1159:1167	arg1	%					1303:1303	80.3 ± 1.0%	1293:1303	80.3 ± 1.0%	1293:1303	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	6	92	dep	decreased	1159:1167	arg1	%					1319:1319	85.3 ± 1.3%	1309:1319	85.3 ± 1.3% for 30 and 40 wt % PLGA content	1309:1351	Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks.
25046733	3	93	theme	poly	694:697	arg1	microparticles					731:744	poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles	531:744	poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles	531:744	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	3	93	theme	poly	694:697	arg1	carriers					760:767	antibiotic carriers	749:767	antibiotic carriers	749:767	To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated.
25046733	2	94	theme	secondary	380:388	arg1	surgeries					398:406	secondary removal surgeries	380:406	secondary removal surgeries	380:406	Polymethylmethacrylate-based SMs present several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation.
27720150	11	0	theme	water	1610:1614	arg1	secretion					1616:1624	greater colonic water secretion	1594:1624	greater colonic water secretion	1594:1624	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	11	1	theme	epithelial	1515:1524	arg1	structures					1526:1535	more developed intestinal epithelial structures	1489:1535	more developed intestinal epithelial structures	1489:1535	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	7	2	theme	commercial	871:880	arg1	replacer					887:894	commercial milk replacer	871:894	commercial milk replacer	871:894	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	3	3	theme	Digestive	200:208	arg1	disorders					210:218	Digestive disorders	200:218	Digestive disorders	200:218	Digestive disorders are common during the first few weeks of life of newborn calves.
27720150	4	4	theme	microbial	395:403	arg1	populations					405:415	beneficial microbial populations	384:415	beneficial microbial populations	384:415	Prebiotics are nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function.
27720150	13	5	theme	epithelium	1762:1771	arg1	resistance					1786:1795	epithelium paracellular resistance	1762:1795	epithelium paracellular resistance	1762:1795	Size of intestinal organs, intestinal nutrient transport, and epithelium paracellular resistance were not affected by treatment.
27720150	7	6	dep	GOS-rich	904:911	arg1	%					923:923	3.4%	920:923	3.4% of dry matter	920:937	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	7	6	dep	GOS-rich	904:911	arg1	matter					932:937	dry matter	928:937	dry matter	928:937	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	3	7	theme	first	242:246	arg1	weeks					252:256	the first few weeks	238:256	the first few weeks of life of newborn calves	238:282	Digestive disorders are common during the first few weeks of life of newborn calves.
27720150	10	8	theme	fecal	1369:1373	arg1	scores					1391:1396	fecal and respiratory scores	1369:1396	fecal and respiratory scores	1369:1396	The remaining 48 calves continued to wk 8 to measure body growth, nutrient intake, and fecal and respiratory scores.
27720150	7	9	theme	control	839:845	arg1	treatment					847:855	a control treatment	837:855	a control treatment consisting of commercial milk replacer	837:894	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	6	10	theme	bacterial	609:617	arg1	composition					629:639	intestinal bacterial community composition	598:639	intestinal bacterial community composition	598:639	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	6	11	theme	growth	777:782	arg1	rates					768:772	high rates	763:772	high rates of growth	763:782	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	3	12	theme	life	261:264	arg1	weeks					252:256	the first few weeks	238:256	the first few weeks of life of newborn calves	238:282	Digestive disorders are common during the first few weeks of life of newborn calves.
27720150	10	13	theme	nutrient	1348:1355	arg1	intake					1357:1362	nutrient intake	1348:1362	nutrient intake	1348:1362	The remaining 48 calves continued to wk 8 to measure body growth, nutrient intake, and fecal and respiratory scores.
27720150	0	14	theme	preweaned	109:117	arg1	calves					128:133	preweaned Holstein calves	109:133	preweaned Holstein calves	109:133	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	4	15	theme	gut	447:449	arg1	health					451:456	enhanced gut health	438:456	enhanced gut health	438:456	Prebiotics are nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function.
27720150	6	16	theme	GOS	575:577	arg1	supplementation					579:593	GOS supplementation	575:593	GOS supplementation	575:593	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	0	17	theme	calves	128:133	arg1	profile					70:76	short-chain fatty acid profile	47:76	short-chain fatty acid profile	47:76	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	0	17	theme	calves	128:133	arg1	community					36:44	the intestinal bacterial community	11:44	the intestinal bacterial community	11:44	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	0	17	theme	calves	128:133	arg1	development					94:104	intestinal development	83:104	intestinal development	83:104	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	9	18	theme	nutrient	1195:1202	arg1	transport					1204:1212	nutrient transport	1195:1212	nutrient transport	1195:1212	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	3	19	theme	newborn	269:275	arg1	calves					277:282	newborn calves	269:282	newborn calves	269:282	Digestive disorders are common during the first few weeks of life of newborn calves.
27720150	11	20	theme	Bifidobacterium	1450:1464	arg1	abundance					1475:1483	greater Lactobacillus and Bifidobacterium relative abundance	1424:1483	greater Lactobacillus and Bifidobacterium relative abundance	1424:1483	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	5	21	theme	prebiotic	525:533	arg1	potential					535:543	prebiotic potential	525:543	prebiotic potential	525:543	Galactooligosaccharides (GOS) have demonstrated such prebiotic potential.
27720150	11	22	theme	Lactobacillus	1432:1444	arg1	abundance					1475:1483	greater Lactobacillus and Bifidobacterium relative abundance	1424:1483	greater Lactobacillus and Bifidobacterium relative abundance	1424:1483	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	0	23	theme	fatty	59:63	arg1	profile					70:76	short-chain fatty acid profile	47:76	short-chain fatty acid profile	47:76	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	7	24	theme	milk	940:943	arg1	treatment					954:962	a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment	902:962	a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment	902:962	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	14	25	theme	Excessive	1829:1837	arg1	supplementation					1843:1857	Excessive GOS supplementation	1829:1857	Excessive GOS supplementation	1829:1857	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	9	26	dep	in	1171:1172	arg1	vitro					1174:1178	vitro	1174:1178	vitro	1174:1178	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	6	27	theme	fermentation	645:656	arg1	profiles					658:665	fermentation profiles	645:665	fermentation profiles	645:665	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	9	28	theme	gastrointestinal	1253:1268	arg1	size					1276:1279	gastrointestinal organ size	1253:1279	gastrointestinal organ size	1253:1279	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	7	29	theme	matter	932:937	arg1	%					923:923	3.4%	920:923	3.4% of dry matter	920:937	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	7	29	theme	matter	932:937	arg1	matter					932:937	dry matter	928:937	dry matter	928:937	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	12	30	theme	dry	1681:1683	arg1	intake					1692:1697	milk dry matter intake	1676:1697	milk dry matter intake	1676:1697	Control calves showed slightly better growth and milk dry matter intake.
27720150	0	31	from	Changes	0:6	arg1	profile					70:76	short-chain fatty acid profile	47:76	short-chain fatty acid profile	47:76	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	0	31	from	Changes	0:6	arg1	community					36:44	the intestinal bacterial community	11:44	the intestinal bacterial community	11:44	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	0	31	from	Changes	0:6	arg1	development					94:104	intestinal development	83:104	intestinal development	83:104	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	10	32	theme	respiratory	1379:1389	arg1	scores					1391:1396	fecal and respiratory scores	1369:1396	fecal and respiratory scores	1369:1396	The remaining 48 calves continued to wk 8 to measure body growth, nutrient intake, and fecal and respiratory scores.
27720150	9	33	theme	fatty	1144:1148	arg1	concentrations					1155:1168	short-chain fatty acid concentrations	1132:1168	short-chain fatty acid concentrations	1132:1168	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	7	34	dep	%	923:923	arg1	i.e.					914:917	i.e.	914:917	i.e.	914:917	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	6	35	theme	intestinal	668:677	arg1	health					679:684	intestinal health	668:684	intestinal health	668:684	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	12	36	theme	better	1658:1663	arg1	growth					1665:1670	slightly better growth	1649:1670	slightly better growth	1649:1670	Control calves showed slightly better growth and milk dry matter intake.
27720150	14	37	theme	intestinal	2005:2014	arg1	epithelium					2016:2025	greater intestinal epithelium	1997:2025	greater intestinal epithelium	1997:2025	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	9	38	theme	acid	1150:1153	arg1	concentrations					1155:1168	short-chain fatty acid concentrations	1132:1168	short-chain fatty acid concentrations	1132:1168	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	39	theme	intestinal	1046:1055	arg1	digesta					1057:1063	intestinal digesta	1046:1063	intestinal digesta	1046:1063	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	7	40	theme	male	792:795	arg1	calves					806:811	Eighty male Holstein calves	785:811	Eighty male Holstein calves	785:811	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	12	41	theme	Control	1627:1633	arg1	calves					1635:1640	Control calves	1627:1640	Control calves	1627:1640	Control calves showed slightly better growth and milk dry matter intake.
27720150	0	42	theme	bacterial	26:34	arg1	community					36:44	the intestinal bacterial community	11:44	the intestinal bacterial community	11:44	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	14	43	theme	bacteria	1973:1980	arg1	population					1982:1991	commensal bacteria population	1963:1991	commensal bacteria population	1963:1991	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	2	44	theme	prebiotic	150:158	arg1	supplementation					160:174	prebiotic supplementation	150:174	prebiotic supplementation	150:174	Effects of prebiotic supplementation depend on site and age.
27720150	6	45	theme	high	763:766	arg1	rates					768:772	high rates	763:772	high rates of growth	763:782	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	11	46	theme	developed	1494:1502	arg1	structures					1526:1535	more developed intestinal epithelial structures	1489:1535	more developed intestinal epithelial structures	1489:1535	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	11	47	theme	fecal	1559:1563	arg1	scores					1565:1570	greater fecal scores	1551:1570	greater fecal scores	1551:1570	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	4	48	theme	nondigestible	300:312	arg1	oligosaccharides					330:345	nondigestible but fermentable oligosaccharides	300:345	nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function	300:469	Prebiotics are nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function.
27720150	4	48	theme	nondigestible	300:312	arg1	Prebiotics					285:294	Prebiotics	285:294	Prebiotics	285:294	Prebiotics are nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function.
27720150	11	49	theme	colonic	1602:1608	arg1	secretion					1616:1624	greater colonic water secretion	1594:1624	greater colonic water secretion	1594:1624	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	14	50	theme	lower	1922:1926	arg1	performance					1935:1945	slightly lower growth performance	1913:1945	slightly lower growth performance	1913:1945	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	4	51	theme	fermentable	318:328	arg1	oligosaccharides					330:345	nondigestible but fermentable oligosaccharides	300:345	nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function	300:469	Prebiotics are nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function.
27720150	4	51	theme	fermentable	318:328	arg1	Prebiotics					285:294	Prebiotics	285:294	Prebiotics	285:294	Prebiotics are nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function.
27720150	13	52	theme	intestinal	1727:1736	arg1	transport					1747:1755	intestinal nutrient transport	1727:1755	intestinal nutrient transport	1727:1755	Size of intestinal organs, intestinal nutrient transport, and epithelium paracellular resistance were not affected by treatment.
27720150	9	53	theme	communities	1119:1129	arg1	measurement					1180:1190	in vitro measurement	1171:1190	in vitro measurement of nutrient transport and permeability	1171:1229	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	53	theme	communities	1119:1129	arg1	concentrations					1155:1168	short-chain fatty acid concentrations	1132:1168	short-chain fatty acid concentrations	1132:1168	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	53	theme	communities	1119:1129	arg1	size					1276:1279	gastrointestinal organ size	1253:1279	gastrointestinal organ size	1253:1279	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	53	theme	communities	1119:1129	arg1	assessment					1095:1104	assessment	1095:1104	assessment of bacterial communities	1095:1129	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	53	theme	communities	1119:1129	arg1	histomorphology					1232:1246	histomorphology	1232:1246	histomorphology	1232:1246	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	4	54	theme	populations	405:415	arg1	activity					372:379	activity	372:379	activity	372:379	Prebiotics are nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function.
27720150	4	54	theme	populations	405:415	arg1	growth					361:366	growth	361:366	growth	361:366	Prebiotics are nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function.
27720150	14	55	contain	had	1859:1861	arg1	supplementation					1843:1857	Excessive GOS supplementation	1829:1857	Excessive GOS supplementation	1829:1857	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	14	55	contain	had	1859:1861	arg2	effects					1891:1897	both prebiotic and laxative effects	1863:1897	both prebiotic and laxative effects	1863:1897	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	9	56	theme	short-chain	1132:1142	arg1	concentrations					1155:1168	short-chain fatty acid concentrations	1132:1168	short-chain fatty acid concentrations	1132:1168	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	7	57	theme	milk	882:885	arg1	replacer					887:894	commercial milk replacer	871:894	commercial milk replacer	871:894	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	13	58	theme	paracellular	1773:1784	arg1	resistance					1786:1795	epithelium paracellular resistance	1762:1795	epithelium paracellular resistance	1762:1795	Size of intestinal organs, intestinal nutrient transport, and epithelium paracellular resistance were not affected by treatment.
27720150	11	59	theme	intestinal	1504:1513	arg1	structures					1526:1535	more developed intestinal epithelial structures	1489:1535	more developed intestinal epithelial structures	1489:1535	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	3	60	theme	few	248:250	arg1	weeks					252:256	the first few weeks	238:256	the first few weeks of life of newborn calves	238:282	Digestive disorders are common during the first few weeks of life of newborn calves.
27720150	13	61	theme	nutrient	1738:1745	arg1	transport					1747:1755	intestinal nutrient transport	1727:1755	intestinal nutrient transport	1727:1755	Size of intestinal organs, intestinal nutrient transport, and epithelium paracellular resistance were not affected by treatment.
27720150	6	62	theme	community	619:627	arg1	composition					629:639	intestinal bacterial community composition	598:639	intestinal bacterial community composition	598:639	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	4	63	theme	beneficial	384:393	arg1	populations					405:415	beneficial microbial populations	384:415	beneficial microbial populations	384:415	Prebiotics are nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function.
27720150	6	64	theme	intestinal	598:607	arg1	composition					629:639	intestinal bacterial community composition	598:639	intestinal bacterial community composition	598:639	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	9	65	theme	in	1171:1172	arg1	measurement					1180:1190	in vitro measurement	1171:1190	in vitro measurement of nutrient transport and permeability	1171:1229	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	0	66	theme	Holstein	119:126	arg1	calves					128:133	preweaned Holstein calves	109:133	preweaned Holstein calves	109:133	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	6	67	theme	supplementation	579:593	arg1	effect					565:570	the effect	561:570	the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth	561:723	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	14	68	theme	laxative	1882:1889	arg1	effects					1891:1897	both prebiotic and laxative effects	1863:1897	both prebiotic and laxative effects	1863:1897	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	13	69	theme	organs	1719:1724	arg1	resistance					1786:1795	epithelium paracellular resistance	1762:1795	epithelium paracellular resistance	1762:1795	Size of intestinal organs, intestinal nutrient transport, and epithelium paracellular resistance were not affected by treatment.
27720150	13	69	theme	organs	1719:1724	arg1	transport					1747:1755	intestinal nutrient transport	1727:1755	intestinal nutrient transport	1727:1755	Size of intestinal organs, intestinal nutrient transport, and epithelium paracellular resistance were not affected by treatment.
27720150	13	69	theme	organs	1719:1724	arg1	Size					1700:1703	Size	1700:1703	Size of intestinal organs	1700:1724	Size of intestinal organs, intestinal nutrient transport, and epithelium paracellular resistance were not affected by treatment.
27720150	9	70	theme	transport	1204:1212	arg1	measurement					1180:1190	in vitro measurement	1171:1190	in vitro measurement of nutrient transport and permeability	1171:1229	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	70	theme	transport	1204:1212	arg1	concentrations					1155:1168	short-chain fatty acid concentrations	1132:1168	short-chain fatty acid concentrations	1132:1168	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	70	theme	transport	1204:1212	arg1	size					1276:1279	gastrointestinal organ size	1253:1279	gastrointestinal organ size	1253:1279	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	70	theme	transport	1204:1212	arg1	assessment					1095:1104	assessment	1095:1104	assessment of bacterial communities	1095:1129	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	70	theme	transport	1204:1212	arg1	histomorphology					1232:1246	histomorphology	1232:1246	histomorphology	1232:1246	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	14	71	theme	prebiotic	1868:1876	arg1	effects					1891:1897	both prebiotic and laxative effects	1863:1897	both prebiotic and laxative effects	1863:1897	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	9	72	theme	permeability	1218:1229	arg1	measurement					1180:1190	in vitro measurement	1171:1190	in vitro measurement of nutrient transport and permeability	1171:1229	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	72	theme	permeability	1218:1229	arg1	concentrations					1155:1168	short-chain fatty acid concentrations	1132:1168	short-chain fatty acid concentrations	1132:1168	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	72	theme	permeability	1218:1229	arg1	size					1276:1279	gastrointestinal organ size	1253:1279	gastrointestinal organ size	1253:1279	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	72	theme	permeability	1218:1229	arg1	assessment					1095:1104	assessment	1095:1104	assessment of bacterial communities	1095:1129	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	72	theme	permeability	1218:1229	arg1	histomorphology					1232:1246	histomorphology	1232:1246	histomorphology	1232:1246	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	12	73	theme	matter	1685:1690	arg1	intake					1692:1697	milk dry matter intake	1676:1697	milk dry matter intake	1676:1697	Control calves showed slightly better growth and milk dry matter intake.
27720150	3	74	theme	calves	277:282	arg1	life					261:264	life	261:264	life of newborn calves	261:282	Digestive disorders are common during the first few weeks of life of newborn calves.
27720150	4	75	theme	enhanced	438:445	arg1	health					451:456	enhanced gut health	438:456	enhanced gut health	438:456	Prebiotics are nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function.
27720150	11	76	theme	greater	1424:1430	arg1	abundance					1475:1483	greater Lactobacillus and Bifidobacterium relative abundance	1424:1483	greater Lactobacillus and Bifidobacterium relative abundance	1424:1483	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	0	77	theme	acid	65:68	arg1	profile					70:76	short-chain fatty acid profile	47:76	short-chain fatty acid profile	47:76	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	14	78	theme	GOS	1839:1841	arg1	supplementation					1843:1857	Excessive GOS supplementation	1829:1857	Excessive GOS supplementation	1829:1857	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	11	79	contain	had	1547:1549	arg1	Calves					1399:1404	Calves	1399:1404	Calves fed GOS	1399:1412	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	11	79	contain	had	1547:1549	arg2	scores					1565:1570	greater fecal scores	1551:1570	greater fecal scores	1551:1570	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	7	80	theme	replacer	945:952	arg1	treatment					954:962	a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment	902:962	a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment	902:962	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	11	81	theme	relative	1466:1473	arg1	abundance					1475:1483	greater Lactobacillus and Bifidobacterium relative abundance	1424:1483	greater Lactobacillus and Bifidobacterium relative abundance	1424:1483	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	0	82	theme	intestinal	83:92	arg1	development					94:104	intestinal development	83:104	intestinal development	83:104	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	9	83	theme	organ	1270:1274	arg1	size					1276:1279	gastrointestinal organ size	1253:1279	gastrointestinal organ size	1253:1279	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	12	84	theme	milk	1676:1679	arg1	intake					1692:1697	milk dry matter intake	1676:1697	milk dry matter intake	1676:1697	Control calves showed slightly better growth and milk dry matter intake.
27720150	7	85	theme	dry	928:930	arg1	matter					932:937	dry matter	928:937	dry matter	928:937	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	14	86	theme	epithelium	2016:2025	arg1	growth					2027:2032	commensal bacteria population and greater intestinal epithelium growth	1963:2032	commensal bacteria population and greater intestinal epithelium growth	1963:2032	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	10	87	theme	remaining	1286:1294	arg1	calves					1299:1304	The remaining 48 calves	1282:1304	The remaining 48 calves	1282:1304	The remaining 48 calves continued to wk 8 to measure body growth, nutrient intake, and fecal and respiratory scores.
27720150	0	88	theme	intestinal	15:24	arg1	community					36:44	the intestinal bacterial community	11:44	the intestinal bacterial community	11:44	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	14	89	theme	greater	1997:2003	arg1	epithelium					2016:2025	greater intestinal epithelium	1997:2025	greater intestinal epithelium	1997:2025	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	6	90	theme	dairy	742:746	arg1	calves					748:753	dairy calves	742:753	dairy calves fed for high rates of growth	742:782	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	9	91	theme	digesta	1057:1063	arg1	tissue					1069:1074	tissue	1069:1074	tissue	1069:1074	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	9	91	theme	digesta	1057:1063	arg1	Samples					1035:1041	Samples	1035:1041	Samples of intestinal digesta	1035:1063	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
27720150	7	92	theme	Holstein	797:804	arg1	calves					806:811	Eighty male Holstein calves	785:811	Eighty male Holstein calves	785:811	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	14	93	theme	population	1982:1991	arg1	growth					2027:2032	commensal bacteria population and greater intestinal epithelium growth	1963:2032	commensal bacteria population and greater intestinal epithelium growth	1963:2032	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	13	94	theme	intestinal	1708:1717	arg1	organs					1719:1724	intestinal organs	1708:1724	intestinal organs	1708:1724	Size of intestinal organs, intestinal nutrient transport, and epithelium paracellular resistance were not affected by treatment.
27720150	2	95	theme	supplementation	160:174	arg1	Effects					139:145	Effects	139:145	Effects of prebiotic supplementation	139:174	Effects of prebiotic supplementation depend on site and age.
27720150	0	96	theme	short-chain	47:57	arg1	profile					70:76	short-chain fatty acid profile	47:76	short-chain fatty acid profile	47:76	Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves.
27720150	7	97	theme	Eighty	785:790	arg1	calves					806:811	Eighty male Holstein calves	785:811	Eighty male Holstein calves	785:811	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	14	98	theme	commensal	1963:1971	arg1	bacteria					1973:1980	commensal bacteria	1963:1980	commensal bacteria population	1963:1991	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	11	99	theme	greater	1551:1557	arg1	scores					1565:1570	greater fecal scores	1551:1570	greater fecal scores	1551:1570	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	7	100	theme	GOS-rich	904:911	arg1	treatment					954:962	a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment	902:962	a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment	902:962	Eighty male Holstein calves were assigned either to a control treatment consisting of commercial milk replacer or to a GOS-rich (i.e., 3.4% of dry matter) milk replacer treatment.
27720150	6	101	from	effect	565:570	arg1	growth					718:723	growth	718:723	growth	718:723	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	6	101	from	effect	565:570	arg1	composition					629:639	intestinal bacterial community composition	598:639	intestinal bacterial community composition	598:639	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	6	101	from	effect	565:570	arg1	profiles					658:665	fermentation profiles	645:665	fermentation profiles	645:665	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	6	101	from	effect	565:570	arg1	health					679:684	intestinal health	668:684	intestinal health	668:684	In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, development, and function; and growth was evaluated in dairy calves fed for high rates of growth.
27720150	11	102	theme	greater	1594:1600	arg1	secretion					1616:1624	greater colonic water secretion	1594:1624	greater colonic water secretion	1594:1624	Calves fed GOS displayed greater Lactobacillus and Bifidobacterium relative abundance and more developed intestinal epithelial structures, but also had greater fecal scores presumably related to greater colonic water secretion.
27720150	14	103	theme	growth	1928:1933	arg1	performance					1935:1945	slightly lower growth performance	1913:1945	slightly lower growth performance	1913:1945	Excessive GOS supplementation had both prebiotic and laxative effects, which led to slightly lower growth performance while promoting commensal bacteria population and greater intestinal epithelium growth.
27720150	10	104	theme	body	1335:1338	arg1	growth					1340:1345	body growth	1335:1345	body growth	1335:1345	The remaining 48 calves continued to wk 8 to measure body growth, nutrient intake, and fecal and respiratory scores.
27720150	9	105	theme	bacterial	1109:1117	arg1	communities					1119:1129	bacterial communities	1109:1129	bacterial communities	1109:1129	Samples of intestinal digesta and tissue were collected for assessment of bacterial communities, short-chain fatty acid concentrations, in vitro measurement of nutrient transport and permeability, histomorphology, and gastrointestinal organ size.
25633939	2	0	theme	orange	364:369	arg1	removal					346:352	Adsorptive removal	335:352	Adsorptive removal of methyl orange (MO) from aqueous solutions	335:397	Adsorptive removal of methyl orange (MO) from aqueous solutions was investigated by batch method.
25633939	0	1	theme	orange	82:87	arg1	removal					64:70	the removal	60:70	the removal of methyl orange from aqueous solution	60:109	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.
25633939	6	2	theme	adsorption	789:798	arg1	capacity					800:807	the maximum adsorption capacity	777:807	the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model	777:860	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	6	2	theme	adsorption	789:798	arg1	mg/g					872:875	224.8 mg/g	866:875	224.8 mg/g	866:875	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	2	3	theme	methyl	357:362	arg1	orange					364:369	methyl orange	357:369	methyl orange (MO)	357:374	Adsorptive removal of methyl orange (MO) from aqueous solutions was investigated by batch method.
25633939	2	3	theme	methyl	357:362	arg1	MO					372:373	MO	372:373	MO	372:373	Adsorptive removal of methyl orange (MO) from aqueous solutions was investigated by batch method.
25633939	2	4	theme	Adsorptive	335:344	arg1	removal					346:352	Adsorptive removal	335:352	Adsorptive removal of methyl orange (MO) from aqueous solutions	335:397	Adsorptive removal of methyl orange (MO) from aqueous solutions was investigated by batch method.
25633939	0	5	from	Utilization	0:10	arg1	solution					102:109	aqueous solution	94:109	aqueous solution	94:109	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.
25633939	0	5	from	Utilization	0:10	arg1	removal					64:70	the removal	60:70	the removal of methyl orange from aqueous solution	60:109	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.
25633939	5	6	theme	Adsorption	608:617	arg1	kinetics					619:626	Adsorption kinetics	608:626	Adsorption kinetics	608:626	Adsorption kinetics was mainly controlled by the pseudo-second-order kinetic model.
25633939	0	7	link	cross-linked	15:26	arg1	composite					47:55	cross-linked chitosan/bentonite composite	15:55	cross-linked chitosan/bentonite composite	15:55	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.
25633939	4	8	theme	minor	558:562	arg1	impact					564:569	only a minor impact	551:569	only a minor impact	551:569	pH value had only a minor impact on MO adsorption in a wide pH range.
25633939	0	9	theme	aqueous	94:100	arg1	solution					102:109	aqueous solution	94:109	aqueous solution	94:109	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.
25633939	5	10	theme	pseudo-second-order	657:675	arg1	model					685:689	the pseudo-second-order kinetic model	653:689	the pseudo-second-order kinetic model	653:689	Adsorption kinetics was mainly controlled by the pseudo-second-order kinetic model.
25633939	3	11	theme	contact	524:530	arg1	time					532:535	contact time	524:535	contact time	524:535	The adsorption of MO onto CCS/BT composite was affected by the ratio of chitosan to BT and contact time.
25633939	6	12	theme	Langmuir	847:854	arg1	model					856:860	the Langmuir model	843:860	the Langmuir model	843:860	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	6	13	theme	Langmuir	748:755	arg1	model					766:770	the Langmuir isotherm model	744:770	the Langmuir isotherm model	744:770	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	6	14	theme	isotherm	757:764	arg1	model					766:770	the Langmuir isotherm model	744:770	the Langmuir isotherm model	744:770	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	1	15	theme	chitosan	172:179	arg1	intercalation					155:167	the intercalation	151:167	the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite	151:332	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	4	16	theme	pH	538:539	arg1	value					541:545	pH value	538:545	pH value	538:545	pH value had only a minor impact on MO adsorption in a wide pH range.
25633939	4	17	from	adsorption	577:586	arg1	range					601:605	a wide pH range	591:605	a wide pH range	591:605	pH value had only a minor impact on MO adsorption in a wide pH range.
25633939	7	18	theme	MO	964:965	arg1	removal					953:959	the removal	949:959	the removal of MO from aqueous solutions	949:988	Experimental results indicated that this adsorbent had a potential for the removal of MO from aqueous solutions.
25633939	6	19	theme	maximum	781:787	arg1	capacity					800:807	the maximum adsorption capacity	777:807	the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model	777:860	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	6	19	theme	maximum	781:787	arg1	mg/g					872:875	224.8 mg/g	866:875	224.8 mg/g	866:875	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	0	20	theme	chitosan/bentonite	28:45	arg1	composite					47:55	cross-linked chitosan/bentonite composite	15:55	cross-linked chitosan/bentonite composite	15:55	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.
25633939	2	21	theme	batch	419:423	arg1	method					425:430	batch method	419:430	batch method	419:430	Adsorptive removal of methyl orange (MO) from aqueous solutions was investigated by batch method.
25633939	0	22	theme	cross-linked	15:26	arg1	composite					47:55	cross-linked chitosan/bentonite composite	15:55	cross-linked chitosan/bentonite composite	15:55	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.
25633939	1	23	theme	cross-linked	283:294	arg1	glutaraldehyde					242:255	glutaraldehyde	242:255	glutaraldehyde	242:255	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	1	23	theme	cross-linked	283:294	arg1	composite					324:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	1	24	with	reaction	216:223	arg1	glutaraldehyde					242:255	glutaraldehyde	242:255	glutaraldehyde	242:255	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	1	24	with	reaction	216:223	arg1	composite					324:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	3	25	theme	CCS/BT	459:464	arg1	composite					466:474	CCS/BT composite	459:474	CCS/BT composite	459:474	The adsorption of MO onto CCS/BT composite was affected by the ratio of chitosan to BT and contact time.
25633939	7	26	contain	had	929:931	arg2	potential					935:943	a potential	933:943	a potential for the removal of MO from aqueous solutions	933:988	Experimental results indicated that this adsorbent had a potential for the removal of MO from aqueous solutions.
25633939	7	26	contain	had	929:931	arg1	adsorbent					919:927	adsorbent	919:927	adsorbent	919:927	Experimental results indicated that this adsorbent had a potential for the removal of MO from aqueous solutions.
25633939	1	27	theme	chitosan/bentonite	296:313	arg1	glutaraldehyde					242:255	glutaraldehyde	242:255	glutaraldehyde	242:255	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	1	27	theme	chitosan/bentonite	296:313	arg1	composite					324:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	5	28	theme	kinetic	677:683	arg1	model					685:689	the pseudo-second-order kinetic model	653:689	the pseudo-second-order kinetic model	653:689	Adsorption kinetics was mainly controlled by the pseudo-second-order kinetic model.
25633939	4	29	theme	pH	598:599	arg1	range					601:605	a wide pH range	591:605	a wide pH range	591:605	pH value had only a minor impact on MO adsorption in a wide pH range.
25633939	1	30	theme	cross-linking	202:214	arg1	reaction					216:223	the cross-linking reaction	198:223	the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite	198:332	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	0	31	theme	composite	47:55	arg1	Utilization					0:10	Utilization	0:10	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.	0:110	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.
25633939	6	32	theme	composite	819:827	arg1	capacity					800:807	the maximum adsorption capacity	777:807	the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model	777:860	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	6	32	theme	composite	819:827	arg1	mg/g					872:875	224.8 mg/g	866:875	224.8 mg/g	866:875	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	7	33	theme	aqueous	972:978	arg1	solutions					980:988	aqueous solutions	972:988	aqueous solutions	972:988	Experimental results indicated that this adsorbent had a potential for the removal of MO from aqueous solutions.
25633939	3	34	theme	MO	451:452	arg1	adsorption					437:446	The adsorption	433:446	The adsorption of MO onto CCS/BT composite	433:474	The adsorption of MO onto CCS/BT composite was affected by the ratio of chitosan to BT and contact time.
25633939	1	35	theme	CCS/BT	316:321	arg1	glutaraldehyde					242:255	glutaraldehyde	242:255	glutaraldehyde	242:255	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	1	35	theme	CCS/BT	316:321	arg1	composite					324:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	6	36	theme	CCS/BT	812:817	arg1	composite					819:827	CCS/BT composite	812:827	CCS/BT composite	812:827	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	4	37	contain	had	547:549	arg2	impact					564:569	only a minor impact	551:569	only a minor impact	551:569	pH value had only a minor impact on MO adsorption in a wide pH range.
25633939	4	37	contain	had	547:549	arg1	value					541:545	pH value	538:545	pH value	538:545	pH value had only a minor impact on MO adsorption in a wide pH range.
25633939	6	38	theme	CCS/BT	718:723	arg1	composite					725:733	CCS/BT composite	718:733	CCS/BT composite	718:733	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	1	39	with	bentonite	184:192	arg1	glutaraldehyde					242:255	glutaraldehyde	242:255	glutaraldehyde	242:255	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	1	39	with	bentonite	184:192	arg1	composite					324:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	2	40	from	solutions	389:397	arg1	removal					346:352	Adsorptive removal	335:352	Adsorptive removal of methyl orange (MO) from aqueous solutions	335:397	Adsorptive removal of methyl orange (MO) from aqueous solutions was investigated by batch method.
25633939	1	41	from	intercalation	155:167	arg1	bentonite					184:192	bentonite	184:192	bentonite	184:192	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	1	41	from	intercalation	155:167	arg1	reaction					216:223	the cross-linking reaction	198:223	the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite	198:332	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	1	42	theme	biocomposite	122:133	arg1	kind					114:117	A kind	112:117	A kind of biocomposite	112:133	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	7	43	theme	Experimental	878:889	arg1	results					891:897	Experimental results	878:897	Experimental results	878:897	Experimental results indicated that this adsorbent had a potential for the removal of MO from aqueous solutions.
25633939	6	44	theme	MO	710:711	arg1	adsorption					696:705	The adsorption	692:705	The adsorption of MO onto CCS/BT composite	692:733	The adsorption of MO onto CCS/BT composite followed the Langmuir isotherm model, and the maximum adsorption capacity of CCS/BT composite calculated by the Langmuir model was 224.8 mg/g.
25633939	0	45	from	solution	102:109	arg1	Utilization					0:10	Utilization	0:10	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.	0:110	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.
25633939	0	45	from	solution	102:109	arg1	removal					64:70	the removal	60:70	the removal of methyl orange from aqueous solution	60:109	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.
25633939	1	46	link	cross-linked	283:294	arg1	glutaraldehyde					242:255	glutaraldehyde	242:255	glutaraldehyde	242:255	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	1	46	link	cross-linked	283:294	arg1	composite					324:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	cross-linked chitosan/bentonite (CCS/BT) composite	283:332	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	1	47	theme	chitosan	228:235	arg1	bentonite					184:192	bentonite	184:192	bentonite	184:192	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	1	47	theme	chitosan	228:235	arg1	reaction					216:223	the cross-linking reaction	198:223	the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite	198:332	A kind of biocomposite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction of chitosan with glutaraldehyde, which was referred to as cross-linked chitosan/bentonite (CCS/BT) composite.
25633939	0	48	theme	methyl	75:80	arg1	orange					82:87	methyl orange	75:87	methyl orange	75:87	Utilization of cross-linked chitosan/bentonite composite in the removal of methyl orange from aqueous solution.
25633939	4	49	theme	wide	593:596	arg1	range					601:605	a wide pH range	591:605	a wide pH range	591:605	pH value had only a minor impact on MO adsorption in a wide pH range.
25633939	2	50	theme	aqueous	381:387	arg1	solutions					389:397	aqueous solutions	381:397	aqueous solutions	381:397	Adsorptive removal of methyl orange (MO) from aqueous solutions was investigated by batch method.
25633939	7	51	from	solutions	980:988	arg1	removal					953:959	the removal	949:959	the removal of MO from aqueous solutions	949:988	Experimental results indicated that this adsorbent had a potential for the removal of MO from aqueous solutions.
25633939	3	52	theme	chitosan	505:512	arg1	ratio					496:500	the ratio	492:500	the ratio of chitosan to BT and contact time	492:535	The adsorption of MO onto CCS/BT composite was affected by the ratio of chitosan to BT and contact time.
25633939	4	53	theme	MO	574:575	arg1	adsorption					577:586	MO adsorption	574:586	MO adsorption in a wide pH range	574:605	pH value had only a minor impact on MO adsorption in a wide pH range.
27387013	4	0	theme	XPS	979:981	arg1	analysis					983:990	XPS analysis	979:990	XPS analysis of the PCL/chitosan core-shell structures	979:1032	XPS analysis of the PCL/chitosan core-shell structures exhibited the characteristic peaks of PCL and chitosan polymers.
27387013	2	1	theme	mechanical	589:598	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	1	theme	mechanical	589:598	arg1	absorption					608:617	mechanical and PBS absorption	589:617	mechanical and PBS absorption	589:617	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	3	2	theme	PCL/chitosan	949:960	arg1	ones					973:976	PCL/chitosan core-shell ones	949:976	PCL/chitosan core-shell ones	949:976	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	3	3	theme	%	875:875	arg1	scaffolds					913:921	66.91% and 61.90% for the PCL and chitosan scaffolds	870:921	66.91% and 61.90% for the PCL and chitosan scaffolds	870:921	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	1	4	theme	core-shell	236:245	arg1	scaffolds					247:255	three dimensional (3D) PCL/chitosan core-shell scaffolds	200:255	three dimensional (3D) PCL/chitosan core-shell scaffolds for tissue engineering applications	200:291	This study was related to combining of synthetic Poly (ε-caprolactone) (PCL) and natural chitosan polymers to develop three dimensional (3D) PCL/chitosan core-shell scaffolds for tissue engineering applications.
27387013	6	5	theme	obtained	1323:1330	arg1	results					1332:1338	The obtained results	1319:1338	The obtained results	1319:1338	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	5	6	theme	CCL-1	1223:1227	arg1	line					1251:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	3	7	theme	inter-fiber	818:828	arg1	size					835:838	the average inter-fiber pore size	806:838	the average inter-fiber pore size values	806:845	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	5	8	theme	MTT	1125:1127	arg1	studies					1116:1122	The cell culture studies	1099:1122	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line	1099:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	8	theme	MTT	1125:1127	arg1	assay					1129:1133	MTT assay	1125:1133	MTT assay	1125:1133	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	9	theme	ATCC	1218:1221	arg1	line					1251:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	10	dep	proved	1256:1261	arg1	performance					1289:1299	the biocompatibility performance	1268:1299	the biocompatibility performance of the scaffolds	1268:1316	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	4	11	theme	characteristic	1048:1061	arg1	peaks					1063:1067	the characteristic peaks	1044:1067	the characteristic peaks of PCL and chitosan polymers	1044:1096	XPS analysis of the PCL/chitosan core-shell structures exhibited the characteristic peaks of PCL and chitosan polymers.
27387013	6	12	dep	viability	1467:1475	arg1	scaffolds					1505:1513	on/within scaffolds	1495:1513	on/within scaffolds	1495:1513	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	5	13	theme	biocompatibility	1272:1287	arg1	performance					1289:1299	the biocompatibility performance	1268:1299	the biocompatibility performance of the scaffolds	1268:1316	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	3	14	theme	%	886:886	arg1	scaffolds					913:921	66.91% and 61.90% for the PCL and chitosan scaffolds	870:921	66.91% and 61.90% for the PCL and chitosan scaffolds	870:921	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	1	15	theme	tissue	261:266	arg1	applications					280:291	tissue engineering applications	261:291	tissue engineering applications	261:291	This study was related to combining of synthetic Poly (ε-caprolactone) (PCL) and natural chitosan polymers to develop three dimensional (3D) PCL/chitosan core-shell scaffolds for tissue engineering applications.
27387013	2	16	theme	electron	513:520	arg1	TEM					534:536	TEM	534:536	TEM	534:536	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	16	theme	electron	513:520	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	16	theme	electron	513:520	arg1	microscopy					522:531	transmission electron microscopy	500:531	transmission electron microscopy (TEM)	500:537	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	17	theme	shrinkage	623:631	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	17	theme	shrinkage	623:631	arg1	tests					633:637	shrinkage tests	623:637	shrinkage tests	623:637	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	4	18	theme	chitosan	1080:1087	arg1	peaks					1063:1067	the characteristic peaks	1044:1067	the characteristic peaks of PCL and chitosan polymers	1044:1096	XPS analysis of the PCL/chitosan core-shell structures exhibited the characteristic peaks of PCL and chitosan polymers.
27387013	2	19	theme	transmission	500:511	arg1	TEM					534:536	TEM	534:536	TEM	534:536	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	19	theme	transmission	500:511	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	19	theme	transmission	500:511	arg1	microscopy					522:531	transmission electron microscopy	500:531	transmission electron microscopy (TEM)	500:537	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	5	20	dep	assay	1129:1133	arg1	analyses					1186:1193	analyses	1186:1193	analyses	1186:1193	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	3	21	theme	average	810:816	arg1	size					835:838	the average inter-fiber pore size	806:838	the average inter-fiber pore size values	806:845	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	1	22	dep	Poly	131:134	arg1	polymers					180:187	polymers	180:187	polymers	180:187	This study was related to combining of synthetic Poly (ε-caprolactone) (PCL) and natural chitosan polymers to develop three dimensional (3D) PCL/chitosan core-shell scaffolds for tissue engineering applications.
27387013	5	23	theme	scaffolds	1308:1316	arg1	performance					1289:1299	the biocompatibility performance	1268:1299	the biocompatibility performance of the scaffolds	1268:1316	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	1	24	theme	natural	163:169	arg1	chitosan					171:178	natural chitosan	163:178	natural chitosan	163:178	This study was related to combining of synthetic Poly (ε-caprolactone) (PCL) and natural chitosan polymers to develop three dimensional (3D) PCL/chitosan core-shell scaffolds for tissue engineering applications.
27387013	4	25	theme	PCL/chitosan	999:1010	arg1	structures					1023:1032	the PCL/chitosan core-shell structures	995:1032	the PCL/chitosan core-shell structures	995:1032	XPS analysis of the PCL/chitosan core-shell structures exhibited the characteristic peaks of PCL and chitosan polymers.
27387013	5	26	theme	cell	1103:1106	arg1	SEM					1182:1184	SEM	1182:1184	SEM	1182:1184	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	26	theme	cell	1103:1106	arg1	studies					1116:1122	The cell culture studies	1099:1122	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line	1099:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	26	theme	cell	1103:1106	arg1	assay					1129:1133	MTT assay	1125:1133	MTT assay	1125:1133	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	26	theme	cell	1103:1106	arg1	Microscope					1160:1169	Confocal Laser Scanning Microscope	1136:1169	Confocal Laser Scanning Microscope (CLSM)	1136:1176	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	2	27	theme	PBS	604:606	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	27	theme	PBS	604:606	arg1	absorption					608:617	mechanical and PBS absorption	589:617	mechanical and PBS absorption	589:617	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	5	28	theme	Scanning	1151:1158	arg1	studies					1116:1122	The cell culture studies	1099:1122	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line	1099:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	28	theme	Scanning	1151:1158	arg1	Microscope					1160:1169	Confocal Laser Scanning Microscope	1136:1169	Confocal Laser Scanning Microscope (CLSM)	1136:1176	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	28	theme	Scanning	1151:1158	arg1	CLSM					1172:1175	CLSM	1172:1175	CLSM	1172:1175	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	29	theme	Laser	1145:1149	arg1	studies					1116:1122	The cell culture studies	1099:1122	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line	1099:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	29	theme	Laser	1145:1149	arg1	Microscope					1160:1169	Confocal Laser Scanning Microscope	1136:1169	Confocal Laser Scanning Microscope (CLSM)	1136:1176	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	29	theme	Laser	1145:1149	arg1	CLSM					1172:1175	CLSM	1172:1175	CLSM	1172:1175	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	6	30	theme	scaffolds	1424:1432	arg1	structure					1383:1391	the created micro/nano fibrous structure	1352:1391	the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study	1352:1446	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	1	31	theme	engineering	268:278	arg1	applications					280:291	tissue engineering applications	261:291	tissue engineering applications	261:291	This study was related to combining of synthetic Poly (ε-caprolactone) (PCL) and natural chitosan polymers to develop three dimensional (3D) PCL/chitosan core-shell scaffolds for tissue engineering applications.
27387013	4	32	dep	PCL	1072:1074	arg1	polymers					1089:1096	polymers	1089:1096	polymers	1089:1096	XPS analysis of the PCL/chitosan core-shell structures exhibited the characteristic peaks of PCL and chitosan polymers.
27387013	6	33	from	structure	1383:1391	arg1	study					1442:1446	this study	1437:1446	this study	1437:1446	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	2	34	theme	samples	396:402	arg1	characterizations					371:387	the characterizations	367:387	the characterizations of the samples	367:402	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	35	theme	CA	446:447	arg1	microscopy					522:531	transmission electron microscopy	500:531	transmission electron microscopy (TEM)	500:537	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	35	theme	CA	446:447	arg1	tests					633:637	shrinkage tests	623:637	shrinkage tests	623:637	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	35	theme	CA	446:447	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	35	theme	CA	446:447	arg1	absorption					608:617	mechanical and PBS absorption	589:617	mechanical and PBS absorption	589:617	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	35	theme	CA	446:447	arg1	analyses					579:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	35	theme	CA	446:447	arg1	microscopy					482:491	scanning electron microscopy	464:491	scanning electron microscopy (SEM)	464:497	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	36	theme	X-Ray	540:544	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	36	theme	X-Ray	540:544	arg1	analyses					579:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	37	theme	angle	439:443	arg1	microscopy					522:531	transmission electron microscopy	500:531	transmission electron microscopy (TEM)	500:537	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	37	theme	angle	439:443	arg1	tests					633:637	shrinkage tests	623:637	shrinkage tests	623:637	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	37	theme	angle	439:443	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	37	theme	angle	439:443	arg1	absorption					608:617	mechanical and PBS absorption	589:617	mechanical and PBS absorption	589:617	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	37	theme	angle	439:443	arg1	analyses					579:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	37	theme	angle	439:443	arg1	microscopy					482:491	scanning electron microscopy	464:491	scanning electron microscopy (SEM)	464:497	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	0	38	theme	core-shell	15:24	arg1	Development					0:10	Development	0:10	Development of core-shell	0:24	Development of core-shell coaxially electrospun composite PCL/chitosan scaffolds.
27387013	6	39	theme	on/within	1495:1503	arg1	scaffolds					1505:1513	on/within scaffolds	1495:1513	on/within scaffolds	1495:1513	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	5	40	theme	mouse	1229:1233	arg1	line					1251:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	2	41	theme	contact	431:437	arg1	microscopy					522:531	transmission electron microscopy	500:531	transmission electron microscopy (TEM)	500:537	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	41	theme	contact	431:437	arg1	tests					633:637	shrinkage tests	623:637	shrinkage tests	623:637	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	41	theme	contact	431:437	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	41	theme	contact	431:437	arg1	absorption					608:617	mechanical and PBS absorption	589:617	mechanical and PBS absorption	589:617	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	41	theme	contact	431:437	arg1	analyses					579:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	41	theme	contact	431:437	arg1	microscopy					482:491	scanning electron microscopy	464:491	scanning electron microscopy (SEM)	464:497	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	0	42	theme	composite	48:56	arg1	scaffolds					71:79	electrospun composite PCL/chitosan scaffolds	36:79	electrospun composite PCL/chitosan scaffolds	36:79	Development of core-shell coaxially electrospun composite PCL/chitosan scaffolds.
27387013	5	43	dep	studies	1116:1122	arg1	SEM					1182:1184	SEM	1182:1184	SEM	1182:1184	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	43	dep	studies	1116:1122	arg1	Microscope					1160:1169	Confocal Laser Scanning Microscope	1136:1169	Confocal Laser Scanning Microscope (CLSM)	1136:1176	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	43	dep	studies	1116:1122	arg1	assay					1129:1133	MTT assay	1125:1133	MTT assay	1125:1133	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	43	dep	studies	1116:1122	arg1	studies					1116:1122	The cell culture studies	1099:1122	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line	1099:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	43	dep	studies	1116:1122	arg1	CLSM					1172:1175	CLSM	1172:1175	CLSM	1172:1175	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	44	theme	fibroblast	1235:1244	arg1	line					1251:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	6	45	theme	cell	1462:1465	arg1	viability					1467:1475	the cell viability	1458:1475	the cell viability	1458:1475	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	0	46	theme	electrospun	36:46	arg1	scaffolds					71:79	electrospun composite PCL/chitosan scaffolds	36:79	electrospun composite PCL/chitosan scaffolds	36:79	Development of core-shell coaxially electrospun composite PCL/chitosan scaffolds.
27387013	3	47	theme	core-shell	763:772	arg1	0.412±0.003μm					785:797	0.412±0.003μm	785:797	0.412±0.003μm	785:797	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	3	47	theme	core-shell	763:772	arg1	scaffolds					774:782	PCL/chitosan core-shell scaffolds	750:782	PCL/chitosan core-shell scaffolds (0.412±0.003μm)	750:798	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	2	48	theme	electron	473:480	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	48	theme	electron	473:480	arg1	SEM					494:496	SEM	494:496	SEM	494:496	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	48	theme	electron	473:480	arg1	microscopy					482:491	scanning electron microscopy	464:491	scanning electron microscopy (SEM)	464:497	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	4	49	theme	structures	1023:1032	arg1	analysis					983:990	XPS analysis	979:990	XPS analysis of the PCL/chitosan core-shell structures	979:1032	XPS analysis of the PCL/chitosan core-shell structures exhibited the characteristic peaks of PCL and chitosan polymers.
27387013	2	50	theme	thickness	417:425	arg1	microscopy					522:531	transmission electron microscopy	500:531	transmission electron microscopy (TEM)	500:537	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	50	theme	thickness	417:425	arg1	tests					633:637	shrinkage tests	623:637	shrinkage tests	623:637	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	50	theme	thickness	417:425	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	50	theme	thickness	417:425	arg1	absorption					608:617	mechanical and PBS absorption	589:617	mechanical and PBS absorption	589:617	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	50	theme	thickness	417:425	arg1	analyses					579:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	50	theme	thickness	417:425	arg1	microscopy					482:491	scanning electron microscopy	464:491	scanning electron microscopy (SEM)	464:497	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	51	theme	scanning	464:471	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	51	theme	scanning	464:471	arg1	SEM					494:496	SEM	494:496	SEM	494:496	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	51	theme	scanning	464:471	arg1	microscopy					482:491	scanning electron microscopy	464:491	scanning electron microscopy (SEM)	464:497	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	52	theme	XPS	574:576	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	52	theme	XPS	574:576	arg1	analyses					579:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	6	53	from	scaffolds	1424:1432	arg1	study					1442:1446	this study	1437:1446	this study	1437:1446	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	1	54	theme	synthetic	121:129	arg1	ε-caprolactone					137:150	ε-caprolactone	137:150	ε-caprolactone	137:150	This study was related to combining of synthetic Poly (ε-caprolactone) (PCL) and natural chitosan polymers to develop three dimensional (3D) PCL/chitosan core-shell scaffolds for tissue engineering applications.
27387013	1	54	theme	synthetic	121:129	arg1	PCL					154:156	PCL	154:156	PCL	154:156	This study was related to combining of synthetic Poly (ε-caprolactone) (PCL) and natural chitosan polymers to develop three dimensional (3D) PCL/chitosan core-shell scaffolds for tissue engineering applications.
27387013	1	54	theme	synthetic	121:129	arg1	Poly					131:134	synthetic Poly	121:134	synthetic Poly (ε-caprolactone) (PCL)	121:157	This study was related to combining of synthetic Poly (ε-caprolactone) (PCL) and natural chitosan polymers to develop three dimensional (3D) PCL/chitosan core-shell scaffolds for tissue engineering applications.
27387013	0	55	theme	PCL/chitosan	58:69	arg1	scaffolds					71:79	electrospun composite PCL/chitosan scaffolds	36:79	electrospun composite PCL/chitosan scaffolds	36:79	Development of core-shell coaxially electrospun composite PCL/chitosan scaffolds.
27387013	5	56	theme	Confocal	1136:1143	arg1	studies					1116:1122	The cell culture studies	1099:1122	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line	1099:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	56	theme	Confocal	1136:1143	arg1	Microscope					1160:1169	Confocal Laser Scanning Microscope	1136:1169	Confocal Laser Scanning Microscope (CLSM)	1136:1176	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	56	theme	Confocal	1136:1143	arg1	CLSM					1172:1175	CLSM	1172:1175	CLSM	1172:1175	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	3	57	theme	diameter	664:671	arg1	values					673:678	The average inter-fiber diameter values	640:678	The average inter-fiber diameter values	640:678	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	3	58	theme	scaffolds	913:921	arg1	decreases					857:865	decreases	857:865	decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds	857:921	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	4	59	theme	PCL	1072:1074	arg1	peaks					1063:1067	the characteristic peaks	1044:1067	the characteristic peaks of PCL and chitosan polymers	1044:1096	XPS analysis of the PCL/chitosan core-shell structures exhibited the characteristic peaks of PCL and chitosan polymers.
27387013	3	60	theme	PCL/chitosan	750:761	arg1	0.412±0.003μm					785:797	0.412±0.003μm	785:797	0.412±0.003μm	785:797	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	3	60	theme	PCL/chitosan	750:761	arg1	scaffolds					774:782	PCL/chitosan core-shell scaffolds	750:782	PCL/chitosan core-shell scaffolds (0.412±0.003μm)	750:798	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	2	61	theme	Spectroscopy	560:571	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	61	theme	Spectroscopy	560:571	arg1	analyses					579:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	6	62	from	study	1442:1446	arg1	structure					1383:1391	the created micro/nano fibrous structure	1352:1391	the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study	1352:1446	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	2	63	theme	electrospinning	337:351	arg1	technique					353:361	coaxial electrospinning technique	329:361	coaxial electrospinning technique	329:361	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	5	64	theme	cell	1246:1249	arg1	line					1251:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	6	65	theme	core-shell	1413:1422	arg1	scaffolds					1424:1432	the PCL/chitosan core-shell scaffolds	1396:1432	the PCL/chitosan core-shell scaffolds in this study	1396:1446	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	2	66	theme	Photoelectron	546:558	arg1	measurements					450:461	thickness and contact angle (CA) measurements	417:461	measurements	450:461	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	2	66	theme	Photoelectron	546:558	arg1	analyses					579:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	X-Ray Photoelectron Spectroscopy (XPS) analyses	540:586	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	3	67	theme	average	644:650	arg1	values					673:678	The average inter-fiber diameter values	640:678	The average inter-fiber diameter values	640:678	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	3	68	theme	core-shell	962:971	arg1	ones					973:976	PCL/chitosan core-shell ones	949:976	PCL/chitosan core-shell ones	949:976	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	2	69	theme	coaxial	329:335	arg1	technique					353:361	coaxial electrospinning technique	329:361	coaxial electrospinning technique	329:361	The scaffolds were fabricated with coaxial electrospinning technique and the characterizations of the samples were done by thickness and contact angle (CA) measurements, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) analyses, mechanical and PBS absorption and shrinkage tests.
27387013	3	70	theme	pore	830:833	arg1	size					835:838	the average inter-fiber pore size	806:838	the average inter-fiber pore size values	806:845	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	6	71	theme	PCL/chitosan	1400:1411	arg1	scaffolds					1424:1432	the PCL/chitosan core-shell scaffolds	1396:1432	the PCL/chitosan core-shell scaffolds in this study	1396:1446	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	6	72	theme	micro/nano	1364:1373	arg1	structure					1383:1391	the created micro/nano fibrous structure	1352:1391	the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study	1352:1446	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	3	73	theme	inter-fiber	652:662	arg1	values					673:678	The average inter-fiber diameter values	640:678	The average inter-fiber diameter values	640:678	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	3	74	dep	PCL	896:898	arg1	the					892:894	the	892:894	the	892:894	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	6	75	theme	fibrous	1375:1381	arg1	structure					1383:1391	the created micro/nano fibrous structure	1352:1391	the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study	1352:1446	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	3	76	theme	size	835:838	arg1	values					840:845	the average inter-fiber pore size values	806:845	the average inter-fiber pore size values	806:845	The average inter-fiber diameter values were calculated for PCL (0.717±0.001μm), chitosan (0.660±0.007μm) and PCL/chitosan core-shell scaffolds (0.412±0.003μm), also the average inter-fiber pore size values exhibited decreases of 66.91% and 61.90% for the PCL and chitosan scaffolds respectively, compared to PCL/chitosan core-shell ones.
27387013	6	77	theme	created	1356:1362	arg1	structure					1383:1391	the created micro/nano fibrous structure	1352:1391	the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study	1352:1446	The obtained results showed that the created micro/nano fibrous structure of the PCL/chitosan core-shell scaffolds in this study increased the cell viability and proliferation on/within scaffolds.
27387013	5	78	theme	culture	1108:1114	arg1	SEM					1182:1184	SEM	1182:1184	SEM	1182:1184	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	78	theme	culture	1108:1114	arg1	studies					1116:1122	The cell culture studies	1099:1122	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line	1099:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	78	theme	culture	1108:1114	arg1	assay					1129:1133	MTT assay	1125:1133	MTT assay	1125:1133	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	5	78	theme	culture	1108:1114	arg1	Microscope					1160:1169	Confocal Laser Scanning Microscope	1136:1169	Confocal Laser Scanning Microscope (CLSM)	1136:1176	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	4	79	theme	core-shell	1012:1021	arg1	structures					1023:1032	the PCL/chitosan core-shell structures	995:1032	the PCL/chitosan core-shell structures	995:1032	XPS analysis of the PCL/chitosan core-shell structures exhibited the characteristic peaks of PCL and chitosan polymers.
27387013	5	80	theme	L929	1213:1216	arg1	line					1251:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	L929 ATCC CCL-1 mouse fibroblast cell line	1213:1254	The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
27387013	1	81	theme	PCL/chitosan	223:234	arg1	scaffolds					247:255	three dimensional (3D) PCL/chitosan core-shell scaffolds	200:255	three dimensional (3D) PCL/chitosan core-shell scaffolds for tissue engineering applications	200:291	This study was related to combining of synthetic Poly (ε-caprolactone) (PCL) and natural chitosan polymers to develop three dimensional (3D) PCL/chitosan core-shell scaffolds for tissue engineering applications.
25497319	0	0	theme	emulsion	80:87	arg1	composition					89:99	emulsion composition	80:99	emulsion composition	80:99	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.
25497319	5	1	theme	AH-8	800:803	arg1	quantification					782:795	quantification	782:795	quantification of AH-8	782:803	By applying LC-MS/MS for quantification of AH-8, we demonstrated that a multiple water-in-oil-in-water (W/O/W) emulsion can significantly increase penetration of AH-8 into porcine skin compared to simple O/W and W/O emulsions.
25497319	3	2	theme	vehicle	488:494	arg1	type					496:499	the vehicle type	484:499	the vehicle type	484:499	Therefore, the aim of the present study was to investigate the influence of the vehicle type on the AH-8 delivery to the skin.
25497319	1	3	theme	topical	243:249	arg1	hexapeptide-8					132:144	Acetyl hexapeptide-8	125:144	Acetyl hexapeptide-8 (AH-8)	125:151	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	1	3	theme	topical	243:249	arg1	treatment					251:259	a promising topical treatment	231:259	a promising topical treatment of blepharospasm	231:276	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	6	4	theme	multiple	1024:1031	arg1	emulsion					1033:1040	the developed multiple emulsion	1010:1040	the developed multiple emulsion	1010:1040	The internal structure of the developed multiple emulsion was confirmed by electron microscopic investigations and NMR self diffusion studies.
25497319	3	5	theme	type	496:499	arg1	influence					471:479	the influence	467:479	the influence of the vehicle type on the AH-8 delivery to the skin	467:532	Therefore, the aim of the present study was to investigate the influence of the vehicle type on the AH-8 delivery to the skin.
25497319	8	6	theme	increased	1330:1338	arg1	absorption					1340:1349	an increased absorption	1327:1349	an increased absorption of the water-rich emulsions into the skin, confirmed by combined ATR-FTIR and tape stripping experiments	1327:1454	This enhanced delivery of AH-8 could be explained by an increased absorption of the water-rich emulsions into the skin, confirmed by combined ATR-FTIR and tape stripping experiments.
25497319	4	7	theme	permeation	548:557	arg1	experiments					559:569	skin permeation experiments	543:569	skin permeation experiments with Franz type diffusion cells	543:601	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	6	8	theme	microscopic	1068:1078	arg1	investigations					1080:1093	electron microscopic investigations	1059:1093	electron microscopic investigations	1059:1093	The internal structure of the developed multiple emulsion was confirmed by electron microscopic investigations and NMR self diffusion studies.
25497319	7	9	from	emulsion	1218:1225	arg1	terms					1230:1234	terms	1230:1234	terms of dermal delivery of AH-8	1230:1261	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	5	10	theme	porcine	929:935	arg1	skin					937:940	porcine skin	929:940	porcine skin	929:940	By applying LC-MS/MS for quantification of AH-8, we demonstrated that a multiple water-in-oil-in-water (W/O/W) emulsion can significantly increase penetration of AH-8 into porcine skin compared to simple O/W and W/O emulsions.
25497319	4	11	theme	Franz	576:580	arg1	cells					597:601	Franz type diffusion cells	576:601	Franz type diffusion cells	576:601	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	0	12	theme	composition	89:99	arg1	influence					67:75	influence	67:75	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.	0:123	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.
25497319	0	13	from	emulsions	56:64	arg1	delivery					8:15	Topical delivery	0:15	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.	0:123	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.
25497319	8	14	theme	AH-8	1300:1303	arg1	delivery					1288:1295	This enhanced delivery	1274:1295	This enhanced delivery of AH-8	1274:1303	This enhanced delivery of AH-8 could be explained by an increased absorption of the water-rich emulsions into the skin, confirmed by combined ATR-FTIR and tape stripping experiments.
25497319	4	15	theme	real	668:671	arg1	application					680:690	a real in-use application	666:690	a real in-use application	666:690	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	1	16	theme	blepharospasm	264:276	arg1	hexapeptide-8					132:144	Acetyl hexapeptide-8	125:144	Acetyl hexapeptide-8 (AH-8)	125:151	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	1	16	theme	blepharospasm	264:276	arg1	treatment					251:259	a promising topical treatment	231:259	a promising topical treatment of blepharospasm	231:276	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	0	17	theme	structure	114:122	arg1	influence					67:75	influence	67:75	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.	0:123	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.
25497319	3	18	theme	AH-8	508:511	arg1	delivery					513:520	the AH-8 delivery	504:520	the AH-8 delivery to the skin	504:532	Therefore, the aim of the present study was to investigate the influence of the vehicle type on the AH-8 delivery to the skin.
25497319	5	19	theme	multiple	829:836	arg1	emulsion					868:875	a multiple water-in-oil-in-water (W/O/W) emulsion	827:875	a multiple water-in-oil-in-water (W/O/W) emulsion	827:875	By applying LC-MS/MS for quantification of AH-8, we demonstrated that a multiple water-in-oil-in-water (W/O/W) emulsion can significantly increase penetration of AH-8 into porcine skin compared to simple O/W and W/O emulsions.
25497319	1	20	theme	well-known	158:167	arg1	hexapeptide-8					132:144	Acetyl hexapeptide-8	125:144	Acetyl hexapeptide-8 (AH-8)	125:151	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	1	20	theme	well-known	158:167	arg1	component					169:177	a well-known component	156:177	a well-known component of anti-aging products	156:200	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	0	21	theme	internal	105:112	arg1	structure					114:122	internal structure	105:122	internal structure	105:122	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.
25497319	6	22	theme	self	1103:1106	arg1	studies					1118:1124	NMR self diffusion studies	1099:1124	NMR self diffusion studies	1099:1124	The internal structure of the developed multiple emulsion was confirmed by electron microscopic investigations and NMR self diffusion studies.
25497319	4	23	theme	porcine	739:745	arg1	skin					751:754	porcine ear skin	739:754	porcine ear skin	739:754	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	6	24	theme	electron	1059:1066	arg1	investigations					1080:1093	electron microscopic investigations	1059:1093	electron microscopic investigations	1059:1093	The internal structure of the developed multiple emulsion was confirmed by electron microscopic investigations and NMR self diffusion studies.
25497319	3	25	theme	present	434:440	arg1	study					442:446	the present study	430:446	the present study	430:446	Therefore, the aim of the present study was to investigate the influence of the vehicle type on the AH-8 delivery to the skin.
25497319	7	26	theme	AH-8	1258:1261	arg1	delivery					1246:1253	dermal delivery	1239:1253	dermal delivery of AH-8	1239:1261	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	3	27	from	influence	471:479	arg1	delivery					513:520	the AH-8 delivery	504:520	the AH-8 delivery to the skin	504:532	Therefore, the aim of the present study was to investigate the influence of the vehicle type on the AH-8 delivery to the skin.
25497319	4	28	theme	in	712:713	arg1	stripping					726:734	in vitro tape stripping	712:734	in vitro tape stripping	712:734	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	7	29	theme	dermal	1239:1244	arg1	delivery					1246:1253	dermal delivery	1239:1253	dermal delivery of AH-8	1239:1261	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	4	30	theme	stratum	644:650	arg1	corneum					652:658	the stratum corneum	640:658	the stratum corneum after a real in-use application	640:690	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	0	31	theme	Topical	0:6	arg1	delivery					8:15	Topical delivery	0:15	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.	0:123	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.
25497319	4	32	dep	in	712:713	arg1	vitro					715:719	vitro	715:719	vitro	715:719	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	5	33	theme	AH-8	919:922	arg1	penetration					904:914	penetration	904:914	penetration of AH-8 into porcine skin	904:940	By applying LC-MS/MS for quantification of AH-8, we demonstrated that a multiple water-in-oil-in-water (W/O/W) emulsion can significantly increase penetration of AH-8 into porcine skin compared to simple O/W and W/O emulsions.
25497319	1	34	theme	anti-aging	182:191	arg1	products					193:200	anti-aging products	182:200	anti-aging products	182:200	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	0	35	theme	acetyl	20:25	arg1	hexapeptide-8					27:39	acetyl hexapeptide-8	20:39	acetyl hexapeptide-8	20:39	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.
25497319	4	36	with	experiments	559:569	arg1	cells					597:601	Franz type diffusion cells	576:601	Franz type diffusion cells	576:601	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	4	37	theme	tape	721:724	arg1	stripping					726:734	in vitro tape stripping	712:734	in vitro tape stripping	712:734	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	4	38	from	distribution	616:627	arg1	corneum					652:658	the stratum corneum	640:658	the stratum corneum after a real in-use application	640:690	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	1	39	theme	products	193:200	arg1	hexapeptide-8					132:144	Acetyl hexapeptide-8	125:144	Acetyl hexapeptide-8 (AH-8)	125:151	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	1	39	theme	products	193:200	arg1	component					169:177	a well-known component	156:177	a well-known component of anti-aging products	156:200	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	6	40	theme	NMR	1099:1101	arg1	studies					1118:1124	NMR self diffusion studies	1099:1124	NMR self diffusion studies	1099:1124	The internal structure of the developed multiple emulsion was confirmed by electron microscopic investigations and NMR self diffusion studies.
25497319	4	41	theme	skin	543:546	arg1	experiments					559:569	skin permeation experiments	543:569	skin permeation experiments with Franz type diffusion cells	543:601	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	4	42	theme	ear	747:749	arg1	skin					751:754	porcine ear skin	739:754	porcine ear skin	739:754	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	2	43	theme	skin	340:343	arg1	penetration					345:355	its skin penetration	336:355	its skin penetration	336:355	Although AH-8 appears in a variety of cosmetic products, its skin penetration is sparsely studied and controversially discussed.
25497319	6	44	theme	developed	1014:1022	arg1	emulsion					1033:1040	the developed multiple emulsion	1010:1040	the developed multiple emulsion	1010:1040	The internal structure of the developed multiple emulsion was confirmed by electron microscopic investigations and NMR self diffusion studies.
25497319	4	45	theme	in-use	673:678	arg1	application					680:690	a real in-use application	666:690	a real in-use application	666:690	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	5	46	dep	O/W	961:963	arg1	emulsions					973:981	emulsions	973:981	emulsions	973:981	By applying LC-MS/MS for quantification of AH-8, we demonstrated that a multiple water-in-oil-in-water (W/O/W) emulsion can significantly increase penetration of AH-8 into porcine skin compared to simple O/W and W/O emulsions.
25497319	5	47	theme	water-in-oil-in-water	838:858	arg1	emulsion					868:875	a multiple water-in-oil-in-water (W/O/W) emulsion	827:875	a multiple water-in-oil-in-water (W/O/W) emulsion	827:875	By applying LC-MS/MS for quantification of AH-8, we demonstrated that a multiple water-in-oil-in-water (W/O/W) emulsion can significantly increase penetration of AH-8 into porcine skin compared to simple O/W and W/O emulsions.
25497319	3	48	theme	study	442:446	arg1	aim					423:425	the aim	419:425	the aim of the present study	419:446	Therefore, the aim of the present study was to investigate the influence of the vehicle type on the AH-8 delivery to the skin.
25497319	0	49	theme	hexapeptide-8	27:39	arg1	delivery					8:15	Topical delivery	0:15	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.	0:123	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.
25497319	8	50	theme	ATR-FTIR	1416:1423	arg1	experiments					1444:1454	combined ATR-FTIR and tape stripping experiments	1407:1454	combined ATR-FTIR and tape stripping experiments	1407:1454	This enhanced delivery of AH-8 could be explained by an increased absorption of the water-rich emulsions into the skin, confirmed by combined ATR-FTIR and tape stripping experiments.
25497319	7	51	theme	W/O/W	1173:1177	arg1	superiority					1147:1157	a clear superiority	1139:1157	a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8	1139:1261	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	8	52	theme	combined	1407:1414	arg1	experiments					1444:1454	combined ATR-FTIR and tape stripping experiments	1407:1454	combined ATR-FTIR and tape stripping experiments	1407:1454	This enhanced delivery of AH-8 could be explained by an increased absorption of the water-rich emulsions into the skin, confirmed by combined ATR-FTIR and tape stripping experiments.
25497319	8	53	theme	emulsions	1369:1377	arg1	absorption					1340:1349	an increased absorption	1327:1349	an increased absorption of the water-rich emulsions into the skin, confirmed by combined ATR-FTIR and tape stripping experiments	1327:1454	This enhanced delivery of AH-8 could be explained by an increased absorption of the water-rich emulsions into the skin, confirmed by combined ATR-FTIR and tape stripping experiments.
25497319	6	54	theme	diffusion	1108:1116	arg1	studies					1118:1124	NMR self diffusion studies	1099:1124	NMR self diffusion studies	1099:1124	The internal structure of the developed multiple emulsion was confirmed by electron microscopic investigations and NMR self diffusion studies.
25497319	7	55	theme	delivery	1246:1253	arg1	terms					1230:1234	terms	1230:1234	terms of dermal delivery of AH-8	1230:1261	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	0	56	theme	different	46:54	arg1	emulsions					56:64	different emulsions	46:64	different emulsions	46:64	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.
25497319	7	57	dep	W/O/W	1173:1177	arg1	emulsions					1187:1195	emulsions	1187:1195	emulsions	1187:1195	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	8	58	theme	tape	1429:1432	arg1	stripping					1434:1442	tape stripping	1429:1442	tape stripping	1429:1442	This enhanced delivery of AH-8 could be explained by an increased absorption of the water-rich emulsions into the skin, confirmed by combined ATR-FTIR and tape stripping experiments.
25497319	8	59	theme	water-rich	1358:1367	arg1	emulsions					1369:1377	the water-rich emulsions	1354:1377	the water-rich emulsions	1354:1377	This enhanced delivery of AH-8 could be explained by an increased absorption of the water-rich emulsions into the skin, confirmed by combined ATR-FTIR and tape stripping experiments.
25497319	7	60	theme	O/W	1183:1185	arg1	superiority					1147:1157	a clear superiority	1139:1157	a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8	1139:1261	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	1	61	theme	Acetyl	125:130	arg1	hexapeptide-8					132:144	Acetyl hexapeptide-8	125:144	Acetyl hexapeptide-8 (AH-8)	125:151	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	1	61	theme	Acetyl	125:130	arg1	treatment					251:259	a promising topical treatment	231:259	a promising topical treatment of blepharospasm	231:276	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	1	61	theme	Acetyl	125:130	arg1	component					169:177	a well-known component	156:177	a well-known component of anti-aging products	156:200	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	1	61	theme	Acetyl	125:130	arg1	AH-8					147:150	AH-8	147:150	AH-8	147:150	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	6	62	theme	internal	988:995	arg1	structure					997:1005	The internal structure	984:1005	The internal structure of the developed multiple emulsion	984:1040	The internal structure of the developed multiple emulsion was confirmed by electron microscopic investigations and NMR self diffusion studies.
25497319	2	63	theme	products	326:333	arg1	variety					306:312	a variety	304:312	a variety of cosmetic products	304:333	Although AH-8 appears in a variety of cosmetic products, its skin penetration is sparsely studied and controversially discussed.
25497319	2	63	theme	products	326:333	arg1	products					326:333	cosmetic products	317:333	cosmetic products	317:333	Although AH-8 appears in a variety of cosmetic products, its skin penetration is sparsely studied and controversially discussed.
25497319	4	64	theme	diffusion	587:595	arg1	cells					597:601	Franz type diffusion cells	576:601	Franz type diffusion cells	576:601	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	6	65	theme	emulsion	1033:1040	arg1	structure					997:1005	The internal structure	984:1005	The internal structure of the developed multiple emulsion	984:1040	The internal structure of the developed multiple emulsion was confirmed by electron microscopic investigations and NMR self diffusion studies.
25497319	7	66	located	found	1267:1271	arg1	general					1130:1136	general	1130:1136	general	1130:1136	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	7	66	located	found	1267:1271	arg2	superiority					1147:1157	a clear superiority	1139:1157	a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8	1139:1261	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	2	67	theme	cosmetic	317:324	arg1	products					326:333	cosmetic products	317:333	cosmetic products	317:333	Although AH-8 appears in a variety of cosmetic products, its skin penetration is sparsely studied and controversially discussed.
25497319	4	68	theme	type	582:585	arg1	cells					597:601	Franz type diffusion cells	576:601	Franz type diffusion cells	576:601	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	8	69	theme	stripping	1434:1442	arg1	experiments					1444:1454	combined ATR-FTIR and tape stripping experiments	1407:1454	combined ATR-FTIR and tape stripping experiments	1407:1454	This enhanced delivery of AH-8 could be explained by an increased absorption of the water-rich emulsions into the skin, confirmed by combined ATR-FTIR and tape stripping experiments.
25497319	0	70	dep	delivery	8:15	arg1	influence					67:75	influence	67:75	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.	0:123	Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.
25497319	4	71	theme	spatial	608:614	arg1	distribution					616:627	the spatial distribution	604:627	the spatial distribution of AH-8 in the stratum corneum after a real in-use application	604:690	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	8	72	theme	enhanced	1279:1286	arg1	delivery					1288:1295	This enhanced delivery	1274:1295	This enhanced delivery of AH-8	1274:1303	This enhanced delivery of AH-8 could be explained by an increased absorption of the water-rich emulsions into the skin, confirmed by combined ATR-FTIR and tape stripping experiments.
25497319	4	73	theme	AH-8	632:635	arg1	distribution					616:627	the spatial distribution	604:627	the spatial distribution of AH-8 in the stratum corneum after a real in-use application	604:690	Besides skin permeation experiments with Franz type diffusion cells, the spatial distribution of AH-8 in the stratum corneum after a real in-use application was investigated by in vitro tape stripping on porcine ear skin.
25497319	7	74	theme	oil-rich	1205:1212	arg1	emulsion					1218:1225	an oil-rich W/O emulsion	1202:1225	an oil-rich W/O emulsion in terms of dermal delivery of AH-8	1202:1261	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	7	75	theme	clear	1141:1145	arg1	superiority					1147:1157	a clear superiority	1139:1157	a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8	1139:1261	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	5	76	theme	W/O/W	861:865	arg1	emulsion					868:875	a multiple water-in-oil-in-water (W/O/W) emulsion	827:875	a multiple water-in-oil-in-water (W/O/W) emulsion	827:875	By applying LC-MS/MS for quantification of AH-8, we demonstrated that a multiple water-in-oil-in-water (W/O/W) emulsion can significantly increase penetration of AH-8 into porcine skin compared to simple O/W and W/O emulsions.
25497319	7	77	theme	W/O	1214:1216	arg1	emulsion					1218:1225	an oil-rich W/O emulsion	1202:1225	an oil-rich W/O emulsion in terms of dermal delivery of AH-8	1202:1261	In general, a clear superiority of water-rich W/O/W and O/W emulsions over an oil-rich W/O emulsion in terms of dermal delivery of AH-8 was found.
25497319	1	78	theme	promising	233:241	arg1	hexapeptide-8					132:144	Acetyl hexapeptide-8	125:144	Acetyl hexapeptide-8 (AH-8)	125:151	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
25497319	1	78	theme	promising	233:241	arg1	treatment					251:259	a promising topical treatment	231:259	a promising topical treatment of blepharospasm	231:276	Acetyl hexapeptide-8 (AH-8) is a well-known component of anti-aging products and was recently explored as a promising topical treatment of blepharospasm.
26767243	1	0	theme	elastic	246:252	arg1	part					254:257	an elastic part	243:257	an elastic part that enables the fabrication of a restoration with proper occlusal anatomy	243:332	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	2	1	theme	laboratory	582:591	arg1	cost					593:596	laboratory cost	582:596	laboratory cost	582:596	The technique overcomes the disadvantages of the direct technique such as polymerization shrinkage stress and difficulty in achieving proper contours and, compared to the indirect technique, treatment is completed in a single appointment, without laboratory cost.
26767243	1	2	with	restoration	293:303	arg1	anatomy					326:332	proper occlusal anatomy	310:332	proper occlusal anatomy	310:332	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	1	3	theme	stone	173:177	arg1	model					179:183	a stone model	171:183	a stone model that is quickly mounted on an articulator	171:225	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	2	4	theme	indirect	506:513	arg1	technique					515:523	the indirect technique	502:523	the indirect technique	502:523	The technique overcomes the disadvantages of the direct technique such as polymerization shrinkage stress and difficulty in achieving proper contours and, compared to the indirect technique, treatment is completed in a single appointment, without laboratory cost.
26767243	1	5	theme	modified	76:83	arg1	technique					102:110	The modified semidirect onlay technique	72:110	The modified semidirect onlay technique with articulated elastic model	72:141	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	1	6	theme	model	179:183	arg1	fabrication					156:166	the fabrication	152:166	the fabrication of a stone model that is quickly mounted on an articulator	152:225	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	0	7	theme	Modified	4:11	arg1	Technique					30:38	The Modified Semidirect Onlay Technique	0:38	The Modified Semidirect Onlay Technique	0:38	The Modified Semidirect Onlay Technique With Articulated Elastic Model.
26767243	1	8	theme	semidirect	85:94	arg1	technique					102:110	The modified semidirect onlay technique	72:110	The modified semidirect onlay technique with articulated elastic model	72:141	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	1	9	theme	onlay	96:100	arg1	technique					102:110	The modified semidirect onlay technique	72:110	The modified semidirect onlay technique with articulated elastic model	72:141	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	0	10	theme	Onlay	24:28	arg1	Technique					30:38	The Modified Semidirect Onlay Technique	0:38	The Modified Semidirect Onlay Technique	0:38	The Modified Semidirect Onlay Technique With Articulated Elastic Model.
26767243	2	11	theme	shrinkage	424:432	arg1	stress					434:439	polymerization shrinkage stress	409:439	polymerization shrinkage stress	409:439	The technique overcomes the disadvantages of the direct technique such as polymerization shrinkage stress and difficulty in achieving proper contours and, compared to the indirect technique, treatment is completed in a single appointment, without laboratory cost.
26767243	0	12	theme	Semidirect	13:22	arg1	Technique					30:38	The Modified Semidirect Onlay Technique	0:38	The Modified Semidirect Onlay Technique	0:38	The Modified Semidirect Onlay Technique With Articulated Elastic Model.
26767243	3	13	theme	occlusal	720:727	arg1	adjustments					729:739	occlusal adjustments	720:739	occlusal adjustments	720:739	The novelty of the technique is that, since the restoration is fabricated on an articulated model it eliminates time for occlusal adjustments.
26767243	2	14	theme	polymerization	409:422	arg1	stress					434:439	polymerization shrinkage stress	409:439	polymerization shrinkage stress	409:439	The technique overcomes the disadvantages of the direct technique such as polymerization shrinkage stress and difficulty in achieving proper contours and, compared to the indirect technique, treatment is completed in a single appointment, without laboratory cost.
26767243	3	15	dep	model	691:695	arg1	eliminates					700:709	eliminates	700:709	eliminates time for occlusal adjustments	700:739	The novelty of the technique is that, since the restoration is fabricated on an articulated model it eliminates time for occlusal adjustments.
26767243	2	16	theme	technique	391:399	arg1	difficulty					445:454	difficulty	445:454	difficulty	445:454	The technique overcomes the disadvantages of the direct technique such as polymerization shrinkage stress and difficulty in achieving proper contours and, compared to the indirect technique, treatment is completed in a single appointment, without laboratory cost.
26767243	2	16	theme	technique	391:399	arg1	stress					434:439	polymerization shrinkage stress	409:439	polymerization shrinkage stress	409:439	The technique overcomes the disadvantages of the direct technique such as polymerization shrinkage stress and difficulty in achieving proper contours and, compared to the indirect technique, treatment is completed in a single appointment, without laboratory cost.
26767243	2	16	theme	technique	391:399	arg1	disadvantages					363:375	the disadvantages	359:375	the disadvantages of the direct technique such as polymerization shrinkage stress and difficulty	359:454	The technique overcomes the disadvantages of the direct technique such as polymerization shrinkage stress and difficulty in achieving proper contours and, compared to the indirect technique, treatment is completed in a single appointment, without laboratory cost.
26767243	0	17	theme	Elastic	57:63	arg1	Model					65:69	Articulated Elastic Model	45:69	Articulated Elastic Model	45:69	The Modified Semidirect Onlay Technique With Articulated Elastic Model.
26767243	1	18	theme	restoration	293:303	arg1	fabrication					276:286	the fabrication	272:286	the fabrication of a restoration with proper occlusal anatomy	272:332	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	1	19	theme	articulated	117:127	arg1	model					137:141	articulated elastic model	117:141	articulated elastic model	117:141	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	0	20	theme	Articulated	45:55	arg1	Model					65:69	Articulated Elastic Model	45:69	Articulated Elastic Model	45:69	The Modified Semidirect Onlay Technique With Articulated Elastic Model.
26767243	3	21	theme	articulated	679:689	arg1	model					691:695	an articulated model	676:695	an articulated model it eliminates time for occlusal adjustments	676:739	The novelty of the technique is that, since the restoration is fabricated on an articulated model it eliminates time for occlusal adjustments.
26767243	0	22	dep	Technique	30:38	arg1	Model					65:69	Articulated Elastic Model	45:69	Articulated Elastic Model	45:69	The Modified Semidirect Onlay Technique With Articulated Elastic Model.
26767243	1	23	theme	elastic	129:135	arg1	model					137:141	articulated elastic model	117:141	articulated elastic model	117:141	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	1	24	theme	proper	310:315	arg1	anatomy					326:332	proper occlusal anatomy	310:332	proper occlusal anatomy	310:332	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	2	25	theme	single	554:559	arg1	appointment					561:571	a single appointment	552:571	a single appointment	552:571	The technique overcomes the disadvantages of the direct technique such as polymerization shrinkage stress and difficulty in achieving proper contours and, compared to the indirect technique, treatment is completed in a single appointment, without laboratory cost.
26767243	3	26	theme	technique	618:626	arg1	that					631:634	that	631:634	that	631:634	The novelty of the technique is that, since the restoration is fabricated on an articulated model it eliminates time for occlusal adjustments.
26767243	3	26	theme	technique	618:626	arg1	novelty					603:609	The novelty	599:609	The novelty of the technique	599:626	The novelty of the technique is that, since the restoration is fabricated on an articulated model it eliminates time for occlusal adjustments.
26767243	2	27	theme	direct	384:389	arg1	technique					391:399	the direct technique	380:399	the direct technique	380:399	The technique overcomes the disadvantages of the direct technique such as polymerization shrinkage stress and difficulty in achieving proper contours and, compared to the indirect technique, treatment is completed in a single appointment, without laboratory cost.
26767243	2	28	theme	proper	469:474	arg1	contours					476:483	proper contours	469:483	proper contours	469:483	The technique overcomes the disadvantages of the direct technique such as polymerization shrinkage stress and difficulty in achieving proper contours and, compared to the indirect technique, treatment is completed in a single appointment, without laboratory cost.
26767243	1	29	theme	occlusal	317:324	arg1	anatomy					326:332	proper occlusal anatomy	310:332	proper occlusal anatomy	310:332	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
26767243	1	30	with	technique	102:110	arg1	model					137:141	articulated elastic model	117:141	articulated elastic model	117:141	The modified semidirect onlay technique with articulated elastic model involves the fabrication of a stone model that is quickly mounted on an articulator and it includes an elastic part that enables the fabrication of a restoration with proper occlusal anatomy.
25666830	2	0	theme	important	108:116	arg1	drug					118:121	an important drug	105:121	an important drug used for treating osteoporosis, which can be produced from vitamin D3	105:191	Calcitriol is an important drug used for treating osteoporosis, which can be produced from vitamin D3.
25666830	2	0	theme	important	108:116	arg1	Calcitriol					91:100	Calcitriol	91:100	Calcitriol	91:100	Calcitriol is an important drug used for treating osteoporosis, which can be produced from vitamin D3.
25666830	3	1	theme	current	198:204	arg1	method					206:211	The current method	194:211	The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells	194:289	The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs.
25666830	3	2	theme	low	302:304	arg1	yields					306:311	low yields	302:311	low yields of calcitriol and high purification costs	302:353	The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs.
25666830	8	3	theme	sodium	963:968	arg1	succinate					970:978	25 mM sodium succinate	957:978	25 mM sodium succinate	957:978	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	8	4	theme	15	938:939	arg1	mM					941:942	mM	941:942	mM	941:942	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	3	5	from	D3	250:251	arg1	calcitriol					226:235	calcitriol	226:235	calcitriol from vitamin D3	226:251	The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs.
25666830	3	6	theme	high	331:334	arg1	costs					349:353	high purification costs	331:353	high purification costs	331:353	The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs.
25666830	9	7	theme	vitamin	1165:1171	arg1	D3					1173:1174	vitamin D3	1165:1174	vitamin D3	1165:1174	Using this optimal bioconversion medium, 61.87 mg/L of calcitriol, corresponding to a 30.94% mass yield from vitamin D3, was produced in a 75-L fermentor after 9 days.
25666830	8	8	theme	2	981:981	arg1	mM					983:984	mM	983:984	mM	983:984	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	9	9	theme	30.94	1142:1146	arg1	%					1147:1147	%	1147:1147	%	1147:1147	Using this optimal bioconversion medium, 61.87 mg/L of calcitriol, corresponding to a 30.94% mass yield from vitamin D3, was produced in a 75-L fermentor after 9 days.
25666830	6	10	theme	high	566:569	arg1	level					571:575	a high level	564:575	a high level	564:575	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	7	11	theme	bioconversion	646:658	arg1	buffers					660:666	bioconversion buffers	646:666	bioconversion buffers	646:666	In addition, the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol were comparatively examined and selected for incorporation in the bioconversion medium, and their compositions were statistically optimized.
25666830	3	12	theme	purification	336:347	arg1	costs					349:353	high purification costs	331:353	high purification costs	331:353	The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs.
25666830	4	13	theme	D3	441:442	arg1	bioconversion					416:428	bioconversion	416:428	bioconversion of vitamin D3 to calcitriol	416:456	Therefore, in this study, the steps of cell cultivation and bioconversion of vitamin D3 to calcitriol were separated.
25666830	4	13	theme	D3	441:442	arg1	cultivation					400:410	cell cultivation	395:410	cell cultivation	395:410	Therefore, in this study, the steps of cell cultivation and bioconversion of vitamin D3 to calcitriol were separated.
25666830	8	14	theme	Trizma	944:949	arg1	base					951:954	15 mM Trizma base	938:954	15 mM Trizma base	938:954	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	7	15	theme	bioconversion	798:810	arg1	medium					812:817	the bioconversion medium	794:817	the bioconversion medium	794:817	In addition, the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol were comparatively examined and selected for incorporation in the bioconversion medium, and their compositions were statistically optimized.
25666830	7	16	theme	buffers	660:666	arg1	effects					635:641	the effects	631:641	the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol	631:730	In addition, the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol were comparatively examined and selected for incorporation in the bioconversion medium, and their compositions were statistically optimized.
25666830	3	17	theme	calcitriol	316:325	arg1	yields					306:311	low yields	302:311	low yields of calcitriol and high purification costs	302:353	The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs.
25666830	4	18	theme	vitamin	433:439	arg1	D3					441:442	vitamin D3	433:442	vitamin D3	433:442	Therefore, in this study, the steps of cell cultivation and bioconversion of vitamin D3 to calcitriol were separated.
25666830	8	19	theme	mM	941:942	arg1	base					951:954	15 mM Trizma base	938:954	15 mM Trizma base	938:954	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	12	20	theme	optimal	1453:1459	arg1	medium					1475:1480	the optimal bioconversion medium	1449:1480	the optimal bioconversion medium	1449:1480	KCTC 1029BP together with the optimal bioconversion medium markedly enhanced the production of calcitriol from vitamin D3.
25666830	6	21	theme	cell	539:542	arg1	1029BP					507:512	KCTC 1029BP	502:512	KCTC 1029BP	502:512	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	6	21	theme	cell	539:542	arg1	catalyst					544:551	a whole cell catalyst	531:551	a whole cell catalyst	531:551	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	12	22	theme	calcitriol	1518:1527	arg1	production					1504:1513	the production	1500:1513	the production of calcitriol from vitamin D3	1500:1543	KCTC 1029BP together with the optimal bioconversion medium markedly enhanced the production of calcitriol from vitamin D3.
25666830	7	23	from	effects	635:641	arg1	production					707:716	the production	703:716	the production of calcitriol	703:730	In addition, the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol were comparatively examined and selected for incorporation in the bioconversion medium, and their compositions were statistically optimized.
25666830	0	24	theme	cell	6:9	arg1	bioconversion					11:23	Whole cell bioconversion	0:23	Whole cell bioconversion of vitamin D3 to calcitriol	0:51	Whole cell bioconversion of vitamin D3 to calcitriol using Pseudonocardia sp.
25666830	6	25	used	utilized	519:526	arg2	1029BP					507:512	KCTC 1029BP	502:512	KCTC 1029BP	502:512	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	6	25	used	utilized	519:526	arg2	catalyst					544:551	a whole cell catalyst	531:551	a whole cell catalyst	531:551	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	8	26	theme	0.08	993:996	arg1	%					997:997	%	997:997	%	997:997	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	11	27	theme	Pseudonocardia	1404:1417	arg1	sp					1419:1420	Pseudonocardia sp	1404:1420	Pseudonocardia sp	1404:1420	In conclusion, utilizing whole cells of Pseudonocardia sp.
25666830	0	28	theme	Whole	0:4	arg1	bioconversion					11:23	Whole cell bioconversion	0:23	Whole cell bioconversion of vitamin D3 to calcitriol	0:51	Whole cell bioconversion of vitamin D3 to calcitriol using Pseudonocardia sp.
25666830	10	29	theme	bioconversion	1294:1306	arg1	medium					1308:1313	a bioconversion medium	1292:1313	a bioconversion medium lacking β-cyclodextrin, NaCl, K2HPO4, and MnCl2	1292:1361	This calcitriol yield was 3.6 times higher than that obtained using a bioconversion medium lacking β-cyclodextrin, NaCl, K2HPO4, and MnCl2.
25666830	8	30	theme	mM	960:961	arg1	succinate					970:978	25 mM sodium succinate	957:978	25 mM sodium succinate	957:978	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	6	31	from	D3	614:615	arg1	yield					581:585	yield	581:585	yield of calcitriol from vitamin D3	581:615	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	6	31	from	D3	614:615	arg1	level					571:575	a high level	564:575	a high level	564:575	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	9	32	theme	calcitriol	1111:1120	arg1	mg/L					1103:1106	61.87 mg/L	1097:1106	61.87 mg/L	1097:1106	Using this optimal bioconversion medium, 61.87 mg/L of calcitriol, corresponding to a 30.94% mass yield from vitamin D3, was produced in a 75-L fermentor after 9 days.
25666830	3	33	theme	costs	349:353	arg1	yields					306:311	low yields	302:311	low yields of calcitriol and high purification costs	302:353	The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs.
25666830	11	34	theme	whole	1389:1393	arg1	cells					1395:1399	whole cells	1389:1399	whole cells of Pseudonocardia sp	1389:1420	In conclusion, utilizing whole cells of Pseudonocardia sp.
25666830	8	35	theme	25	957:958	arg1	mM					960:961	mM	960:961	mM	960:961	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	12	36	theme	bioconversion	1461:1473	arg1	medium					1475:1480	the optimal bioconversion medium	1449:1480	the optimal bioconversion medium	1449:1480	KCTC 1029BP together with the optimal bioconversion medium markedly enhanced the production of calcitriol from vitamin D3.
25666830	7	37	theme	calcitriol	721:730	arg1	production					707:716	the production	703:716	the production of calcitriol	703:730	In addition, the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol were comparatively examined and selected for incorporation in the bioconversion medium, and their compositions were statistically optimized.
25666830	8	38	theme	%	997:997	arg1	β-cyclodextrin					999:1012	0.08% β-cyclodextrin	993:1012	0.08% β-cyclodextrin	993:1012	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	9	39	theme	optimal	1067:1073	arg1	medium					1089:1094	this optimal bioconversion medium	1062:1094	this optimal bioconversion medium	1062:1094	Using this optimal bioconversion medium, 61.87 mg/L of calcitriol, corresponding to a 30.94% mass yield from vitamin D3, was produced in a 75-L fermentor after 9 days.
25666830	0	40	theme	D3	36:37	arg1	bioconversion					11:23	Whole cell bioconversion	0:23	Whole cell bioconversion of vitamin D3 to calcitriol	0:51	Whole cell bioconversion of vitamin D3 to calcitriol using Pseudonocardia sp.
25666830	3	41	theme	vitamin	242:248	arg1	D3					250:251	vitamin D3	242:251	vitamin D3	242:251	The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs.
25666830	4	42	theme	cultivation	400:410	arg1	steps					386:390	the steps	382:390	the steps of cell cultivation and bioconversion of vitamin D3 to calcitriol	382:456	Therefore, in this study, the steps of cell cultivation and bioconversion of vitamin D3 to calcitriol were separated.
25666830	9	43	theme	75-L	1195:1198	arg1	fermentor					1200:1208	a 75-L fermentor	1193:1208	a 75-L fermentor	1193:1208	Using this optimal bioconversion medium, 61.87 mg/L of calcitriol, corresponding to a 30.94% mass yield from vitamin D3, was produced in a 75-L fermentor after 9 days.
25666830	0	44	theme	vitamin	28:34	arg1	D3					36:37	vitamin D3	28:37	vitamin D3	28:37	Whole cell bioconversion of vitamin D3 to calcitriol using Pseudonocardia sp.
25666830	6	45	theme	whole	533:537	arg1	1029BP					507:512	KCTC 1029BP	502:512	KCTC 1029BP	502:512	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	6	45	theme	whole	533:537	arg1	catalyst					544:551	a whole cell catalyst	531:551	a whole cell catalyst	531:551	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	9	46	from	D3	1173:1174	arg1	yield					1154:1158	a 30.94% mass yield	1140:1158	a 30.94% mass yield from vitamin D3	1140:1174	Using this optimal bioconversion medium, 61.87 mg/L of calcitriol, corresponding to a 30.94% mass yield from vitamin D3, was produced in a 75-L fermentor after 9 days.
25666830	8	47	theme	optimal	877:883	arg1	medium					899:904	The optimal bioconversion medium	873:904	The optimal bioconversion medium	873:904	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	9	48	theme	bioconversion	1075:1087	arg1	medium					1089:1094	this optimal bioconversion medium	1062:1094	this optimal bioconversion medium	1062:1094	Using this optimal bioconversion medium, 61.87 mg/L of calcitriol, corresponding to a 30.94% mass yield from vitamin D3, was produced in a 75-L fermentor after 9 days.
25666830	8	49	theme	%	1047:1047	arg1	MnCl2					1049:1053	0.03% MnCl2	1043:1053	0.03% MnCl2	1043:1053	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	3	50	theme	cells	285:289	arg1	cultivation					260:270	cultivation	260:270	cultivation of microbial cells	260:289	The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs.
25666830	4	51	theme	bioconversion	416:428	arg1	steps					386:390	the steps	382:390	the steps of cell cultivation and bioconversion of vitamin D3 to calcitriol	382:456	Therefore, in this study, the steps of cell cultivation and bioconversion of vitamin D3 to calcitriol were separated.
25666830	6	52	theme	KCTC	502:505	arg1	1029BP					507:512	KCTC 1029BP	502:512	KCTC 1029BP	502:512	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	6	52	theme	KCTC	502:505	arg1	catalyst					544:551	a whole cell catalyst	531:551	a whole cell catalyst	531:551	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	8	53	theme	0.2	1026:1028	arg1	%					1029:1029	%	1029:1029	%	1029:1029	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	7	54	theme	cyclodextrins	669:681	arg1	effects					635:641	the effects	631:641	the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol	631:730	In addition, the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol were comparatively examined and selected for incorporation in the bioconversion medium, and their compositions were statistically optimized.
25666830	8	55	theme	%	1018:1018	arg1	NaCl					1020:1023	0.1% NaCl	1015:1023	0.1% NaCl	1015:1023	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	7	56	from	incorporation	777:789	arg1	medium					812:817	the bioconversion medium	794:817	the bioconversion medium	794:817	In addition, the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol were comparatively examined and selected for incorporation in the bioconversion medium, and their compositions were statistically optimized.
25666830	11	57	theme	sp	1419:1420	arg1	cells					1395:1399	whole cells	1389:1399	whole cells of Pseudonocardia sp	1389:1420	In conclusion, utilizing whole cells of Pseudonocardia sp.
25666830	8	58	theme	mM	983:984	arg1	MgSO4					986:990	2 mM MgSO4	981:990	2 mM MgSO4	981:990	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	6	59	theme	vitamin	606:612	arg1	D3					614:615	vitamin D3	606:615	vitamin D3	606:615	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	12	60	theme	KCTC	1423:1426	arg1	1029BP					1428:1433	KCTC 1029BP	1423:1433	KCTC 1029BP together with the optimal bioconversion medium	1423:1480	KCTC 1029BP together with the optimal bioconversion medium markedly enhanced the production of calcitriol from vitamin D3.
25666830	8	61	theme	0.1	1015:1017	arg1	%					1018:1018	%	1018:1018	%	1018:1018	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	2	62	theme	vitamin	182:188	arg1	D3					190:191	vitamin D3	182:191	vitamin D3	182:191	Calcitriol is an important drug used for treating osteoporosis, which can be produced from vitamin D3.
25666830	8	63	theme	bioconversion	885:897	arg1	medium					899:904	The optimal bioconversion medium	873:904	The optimal bioconversion medium	873:904	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	7	64	theme	metal	688:692	arg1	salts					694:698	metal salts	688:698	metal salts	688:698	In addition, the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol were comparatively examined and selected for incorporation in the bioconversion medium, and their compositions were statistically optimized.
25666830	3	65	theme	microbial	275:283	arg1	cells					285:289	microbial cells	275:289	microbial cells	275:289	The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs.
25666830	4	66	theme	cell	395:398	arg1	cultivation					400:410	cell cultivation	395:410	cell cultivation	395:410	Therefore, in this study, the steps of cell cultivation and bioconversion of vitamin D3 to calcitriol were separated.
25666830	12	67	from	D3	1542:1543	arg1	production					1504:1513	the production	1500:1513	the production of calcitriol from vitamin D3	1500:1543	KCTC 1029BP together with the optimal bioconversion medium markedly enhanced the production of calcitriol from vitamin D3.
25666830	6	68	theme	calcitriol	590:599	arg1	yield					581:585	yield	581:585	yield of calcitriol from vitamin D3	581:615	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	6	68	theme	calcitriol	590:599	arg1	level					571:575	a high level	564:575	a high level	564:575	KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3.
25666830	7	69	theme	salts	694:698	arg1	effects					635:641	the effects	631:641	the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol	631:730	In addition, the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol were comparatively examined and selected for incorporation in the bioconversion medium, and their compositions were statistically optimized.
25666830	0	70	theme	Pseudonocardia	59:72	arg1	sp					74:75	Pseudonocardia sp	59:75	Pseudonocardia sp	59:75	Whole cell bioconversion of vitamin D3 to calcitriol using Pseudonocardia sp.
25666830	9	71	theme	%	1147:1147	arg1	yield					1154:1158	a 30.94% mass yield	1140:1158	a 30.94% mass yield from vitamin D3	1140:1174	Using this optimal bioconversion medium, 61.87 mg/L of calcitriol, corresponding to a 30.94% mass yield from vitamin D3, was produced in a 75-L fermentor after 9 days.
25666830	5	72	theme	Pseudonocardia	483:496	arg1	sp					498:499	Pseudonocardia sp	483:499	Pseudonocardia sp	483:499	Cells of Pseudonocardia sp.
25666830	10	73	theme	calcitriol	1229:1238	arg1	yield					1240:1244	This calcitriol yield	1224:1244	This calcitriol yield	1224:1244	This calcitriol yield was 3.6 times higher than that obtained using a bioconversion medium lacking β-cyclodextrin, NaCl, K2HPO4, and MnCl2.
25666830	8	74	theme	%	1029:1029	arg1	K2HPO4					1031:1036	0.2% K2HPO4	1026:1036	0.2% K2HPO4	1026:1036	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
25666830	9	75	theme	mass	1149:1152	arg1	yield					1154:1158	a 30.94% mass yield	1140:1158	a 30.94% mass yield from vitamin D3	1140:1174	Using this optimal bioconversion medium, 61.87 mg/L of calcitriol, corresponding to a 30.94% mass yield from vitamin D3, was produced in a 75-L fermentor after 9 days.
25666830	5	76	theme	sp	498:499	arg1	Cells					474:478	Cells	474:478	Cells of Pseudonocardia sp.	474:500	Cells of Pseudonocardia sp.
25666830	12	77	theme	vitamin	1534:1540	arg1	D3					1542:1543	vitamin D3	1534:1543	vitamin D3	1534:1543	KCTC 1029BP together with the optimal bioconversion medium markedly enhanced the production of calcitriol from vitamin D3.
25666830	8	78	theme	0.03	1043:1046	arg1	%					1047:1047	%	1047:1047	%	1047:1047	The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08% β-cyclodextrin, 0.1% NaCl, 0.2% K2HPO4, and 0.03% MnCl2.
24957293	5	0	theme	stable	732:737	arg1	intensity					751:759	stable fluorescent intensity	732:759	stable fluorescent intensity	732:759	The newly fabricated fluorescent DNG (FDNG) could be designed with optimum size and stable fluorescent intensity for specific SLN imaging.
24957293	6	1	theme	satisfactory	869:880	arg1	specificity					882:892	satisfactory specificity	869:892	satisfactory specificity	869:892	Furthermore, a long-term dynamic course in vivo (from 1 min to 72 h) revealed the satisfactory specificity, sensitivity, and stability for SLN mapping.
24957293	2	2	theme	SLN	319:321	arg1	agents					331:336	ideal, biosafe SLN imaging agents	304:336	ideal, biosafe SLN imaging agents	304:336	Due to many drawbacks of SLN tracers currently used, developing ideal, biosafe SLN imaging agents is always an urgent issue.
24957293	5	3	theme	optimum	715:721	arg1	size					723:726	optimum size	715:726	optimum size	715:726	The newly fabricated fluorescent DNG (FDNG) could be designed with optimum size and stable fluorescent intensity for specific SLN imaging.
24957293	9	4	theme	potential	1515:1523	arg1	application					1525:1535	a potential application	1513:1535	a potential application for specific therapies of various lymph-associated diseases	1513:1595	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	5	5	theme	fluorescent	739:749	arg1	intensity					751:759	stable fluorescent intensity	732:759	stable fluorescent intensity	732:759	The newly fabricated fluorescent DNG (FDNG) could be designed with optimum size and stable fluorescent intensity for specific SLN imaging.
24957293	8	6	theme	specific	1190:1197	arg1	mapping					1246:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	9	7	theme	controllable	1475:1486	arg1	carrier					1493:1499	a specific and controllable drug carrier	1460:1499	a specific and controllable drug carrier	1460:1499	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	9	7	theme	controllable	1475:1486	arg1	DNG					1436:1438	DNG	1436:1438	DNG	1436:1438	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	6	8	dep	revealed	856:863	arg1	72 h					850:853	72 h	850:853	72 h	850:853	Furthermore, a long-term dynamic course in vivo (from 1 min to 72 h) revealed the satisfactory specificity, sensitivity, and stability for SLN mapping.
24957293	9	9	theme	diseases	1588:1595	arg1	therapies					1550:1558	specific therapies	1541:1558	specific therapies of various lymph-associated diseases	1541:1595	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	9	10	theme	drug	1488:1491	arg1	carrier					1493:1499	a specific and controllable drug carrier	1460:1499	a specific and controllable drug carrier	1460:1499	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	9	10	theme	drug	1488:1491	arg1	DNG					1436:1438	DNG	1436:1438	DNG	1436:1438	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	2	11	dep	ideal	304:308	arg1	biosafe					311:317	biosafe	311:317	biosafe	311:317	Due to many drawbacks of SLN tracers currently used, developing ideal, biosafe SLN imaging agents is always an urgent issue.
24957293	6	12	dep	specificity	882:892	arg1	the					865:867	the	865:867	the	865:867	Furthermore, a long-term dynamic course in vivo (from 1 min to 72 h) revealed the satisfactory specificity, sensitivity, and stability for SLN mapping.
24957293	8	13	theme	accurate	1284:1291	arg1	location					1293:1300	efficient and accurate location	1270:1300	efficient and accurate location	1270:1300	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	8	14	theme	efficient	1151:1159	arg1	FDNG					1122:1125	FDNG	1122:1125	FDNG	1122:1125	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	8	14	theme	efficient	1151:1159	arg1	probes					1179:1184	highly efficient molecular imaging probes	1144:1184	highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies	1144:1379	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	7	15	theme	lymphatic	1060:1068	arg1	cells					1082:1086	lymphatic endothelial cells	1060:1086	lymphatic endothelial cells	1060:1086	Most importantly, both in vitro and in vivo evaluations indicated that FDNG had fine biosafety and biocompatibility with lymphatic endothelial cells.
24957293	8	16	theme	SLN	1242:1244	arg1	mapping					1246:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	2	17	theme	many	247:250	arg1	drawbacks					252:260	many drawbacks	247:260	many drawbacks of SLN tracers currently used	247:290	Due to many drawbacks of SLN tracers currently used, developing ideal, biosafe SLN imaging agents is always an urgent issue.
24957293	9	18	theme	recent	1395:1400	arg1	studies					1402:1408	our recent studies	1391:1408	our recent studies	1391:1408	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	2	19	theme	urgent	351:356	arg1	issue					358:362	an urgent issue	348:362	an urgent issue	348:362	Due to many drawbacks of SLN tracers currently used, developing ideal, biosafe SLN imaging agents is always an urgent issue.
24957293	5	20	theme	SLN	774:776	arg1	imaging					778:784	specific SLN imaging	765:784	specific SLN imaging	765:784	The newly fabricated fluorescent DNG (FDNG) could be designed with optimum size and stable fluorescent intensity for specific SLN imaging.
24957293	4	21	theme	Dextran-based	448:460	arg1	platform					581:588	a multi-functional platform	562:588	a multi-functional platform for conjugating wide spectra emitting fluorescent agents	562:645	Dextran-based nanogel (DNG) was prepared through a highly efficient self-assembly assisted approach and serves as a multi-functional platform for conjugating wide spectra emitting fluorescent agents.
24957293	4	21	theme	Dextran-based	448:460	arg1	DNG					471:473	DNG	471:473	DNG	471:473	Dextran-based nanogel (DNG) was prepared through a highly efficient self-assembly assisted approach and serves as a multi-functional platform for conjugating wide spectra emitting fluorescent agents.
24957293	4	21	theme	Dextran-based	448:460	arg1	nanogel					462:468	Dextran-based nanogel	448:468	Dextran-based nanogel (DNG)	448:474	Dextran-based nanogel (DNG) was prepared through a highly efficient self-assembly assisted approach and serves as a multi-functional platform for conjugating wide spectra emitting fluorescent agents.
24957293	7	22	theme	endothelial	1070:1080	arg1	cells					1082:1086	lymphatic endothelial cells	1060:1086	lymphatic endothelial cells	1060:1086	Most importantly, both in vitro and in vivo evaluations indicated that FDNG had fine biosafety and biocompatibility with lymphatic endothelial cells.
24957293	1	23	theme	sentinel	80:87	arg1	node					95:98	sentinel lymph node	80:98	sentinel lymph node (SLN)	80:104	Biopsy of sentinel lymph node (SLN) has become a common practice to predict whether tumor metastasis has occurred, so proper SLN positioning tracers are highly required.
24957293	1	23	theme	sentinel	80:87	arg1	SLN					101:103	SLN	101:103	SLN	101:103	Biopsy of sentinel lymph node (SLN) has become a common practice to predict whether tumor metastasis has occurred, so proper SLN positioning tracers are highly required.
24957293	1	24	theme	tumor	154:158	arg1	metastasis					160:169	tumor metastasis	154:169	tumor metastasis	154:169	Biopsy of sentinel lymph node (SLN) has become a common practice to predict whether tumor metastasis has occurred, so proper SLN positioning tracers are highly required.
24957293	0	25	theme	fluorescent	14:24	arg1	nanoprobes					26:35	Dextran-based fluorescent nanoprobes	0:35	Dextran-based fluorescent nanoprobes for sentinel lymph node mapping.	0:68	Dextran-based fluorescent nanoprobes for sentinel lymph node mapping.
24957293	4	26	theme	fluorescent	628:638	arg1	agents					640:645	fluorescent agents	628:645	fluorescent agents	628:645	Dextran-based nanogel (DNG) was prepared through a highly efficient self-assembly assisted approach and serves as a multi-functional platform for conjugating wide spectra emitting fluorescent agents.
24957293	1	27	theme	lymph	89:93	arg1	node					95:98	sentinel lymph node	80:98	sentinel lymph node (SLN)	80:104	Biopsy of sentinel lymph node (SLN) has become a common practice to predict whether tumor metastasis has occurred, so proper SLN positioning tracers are highly required.
24957293	1	27	theme	lymph	89:93	arg1	SLN					101:103	SLN	101:103	SLN	101:103	Biopsy of sentinel lymph node (SLN) has become a common practice to predict whether tumor metastasis has occurred, so proper SLN positioning tracers are highly required.
24957293	9	28	theme	specific	1541:1548	arg1	therapies					1550:1558	specific therapies	1541:1558	specific therapies of various lymph-associated diseases	1541:1595	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	0	29	theme	Dextran-based	0:12	arg1	nanoprobes					26:35	Dextran-based fluorescent nanoprobes	0:35	Dextran-based fluorescent nanoprobes for sentinel lymph node mapping.	0:68	Dextran-based fluorescent nanoprobes for sentinel lymph node mapping.
24957293	4	30	theme	efficient	506:514	arg1	approach					539:546	a highly efficient self-assembly assisted approach	497:546	a highly efficient self-assembly assisted approach	497:546	Dextran-based nanogel (DNG) was prepared through a highly efficient self-assembly assisted approach and serves as a multi-functional platform for conjugating wide spectra emitting fluorescent agents.
24957293	1	31	theme	node	95:98	arg1	Biopsy					70:75	Biopsy	70:75	Biopsy of sentinel lymph node (SLN)	70:104	Biopsy of sentinel lymph node (SLN) has become a common practice to predict whether tumor metastasis has occurred, so proper SLN positioning tracers are highly required.
24957293	8	32	theme	non-invasive	1219:1230	arg1	mapping					1246:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	8	33	theme	stable	1211:1216	arg1	mapping					1246:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	8	34	theme	imaging	1171:1177	arg1	FDNG					1122:1125	FDNG	1122:1125	FDNG	1122:1125	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	8	34	theme	imaging	1171:1177	arg1	probes					1179:1184	highly efficient molecular imaging probes	1144:1184	highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies	1144:1379	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	6	35	theme	SLN	926:928	arg1	mapping					930:936	SLN mapping	926:936	SLN mapping	926:936	Furthermore, a long-term dynamic course in vivo (from 1 min to 72 h) revealed the satisfactory specificity, sensitivity, and stability for SLN mapping.
24957293	2	36	theme	tracers	269:275	arg1	drawbacks					252:260	many drawbacks	247:260	many drawbacks of SLN tracers currently used	247:290	Due to many drawbacks of SLN tracers currently used, developing ideal, biosafe SLN imaging agents is always an urgent issue.
24957293	7	37	contain	had	1015:1017	arg2	biosafety					1024:1032	fine biosafety	1019:1032	fine biosafety	1019:1032	Most importantly, both in vitro and in vivo evaluations indicated that FDNG had fine biosafety and biocompatibility with lymphatic endothelial cells.
24957293	7	37	contain	had	1015:1017	arg2	biocompatibility					1038:1053	biocompatibility	1038:1053	biocompatibility	1038:1053	Most importantly, both in vitro and in vivo evaluations indicated that FDNG had fine biosafety and biocompatibility with lymphatic endothelial cells.
24957293	7	37	contain	had	1015:1017	arg1	FDNG					1010:1013	FDNG	1010:1013	FDNG	1010:1013	Most importantly, both in vitro and in vivo evaluations indicated that FDNG had fine biosafety and biocompatibility with lymphatic endothelial cells.
24957293	4	38	theme	wide	606:609	arg1	spectra					611:617	wide spectra	606:617	wide spectra emitting fluorescent agents	606:645	Dextran-based nanogel (DNG) was prepared through a highly efficient self-assembly assisted approach and serves as a multi-functional platform for conjugating wide spectra emitting fluorescent agents.
24957293	8	39	theme	molecular	1161:1169	arg1	FDNG					1122:1125	FDNG	1122:1125	FDNG	1122:1125	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	8	39	theme	molecular	1161:1169	arg1	probes					1179:1184	highly efficient molecular imaging probes	1144:1184	highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies	1144:1379	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	4	40	theme	assisted	530:537	arg1	approach					539:546	a highly efficient self-assembly assisted approach	497:546	a highly efficient self-assembly assisted approach	497:546	Dextran-based nanogel (DNG) was prepared through a highly efficient self-assembly assisted approach and serves as a multi-functional platform for conjugating wide spectra emitting fluorescent agents.
24957293	4	41	theme	multi-functional	564:579	arg1	platform					581:588	a multi-functional platform	562:588	a multi-functional platform for conjugating wide spectra emitting fluorescent agents	562:645	Dextran-based nanogel (DNG) was prepared through a highly efficient self-assembly assisted approach and serves as a multi-functional platform for conjugating wide spectra emitting fluorescent agents.
24957293	4	41	theme	multi-functional	564:579	arg1	nanogel					462:468	Dextran-based nanogel	448:468	Dextran-based nanogel (DNG)	448:474	Dextran-based nanogel (DNG) was prepared through a highly efficient self-assembly assisted approach and serves as a multi-functional platform for conjugating wide spectra emitting fluorescent agents.
24957293	2	42	theme	SLN	265:267	arg1	tracers					269:275	SLN tracers	265:275	SLN tracers currently used	265:290	Due to many drawbacks of SLN tracers currently used, developing ideal, biosafe SLN imaging agents is always an urgent issue.
24957293	3	43	theme	accurate	426:433	arg1	mapping					439:445	accurate SLN mapping	426:445	accurate SLN mapping	426:445	The current study designed a novel fluorescent nanoprobe for accurate SLN mapping.
24957293	0	44	theme	lymph	50:54	arg1	node					56:59	sentinel lymph node	41:59	sentinel lymph node mapping	41:67	Dextran-based fluorescent nanoprobes for sentinel lymph node mapping.
24957293	4	45	theme	self-assembly	516:528	arg1	approach					539:546	a highly efficient self-assembly assisted approach	497:546	a highly efficient self-assembly assisted approach	497:546	Dextran-based nanogel (DNG) was prepared through a highly efficient self-assembly assisted approach and serves as a multi-functional platform for conjugating wide spectra emitting fluorescent agents.
24957293	8	46	theme	safe	1237:1240	arg1	mapping					1246:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	8	47	used	used	1136:1139	arg2	FDNG					1122:1125	FDNG	1122:1125	FDNG	1122:1125	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	8	47	used	used	1136:1139	arg2	probes					1179:1184	highly efficient molecular imaging probes	1144:1184	highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies	1144:1379	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	3	48	theme	fluorescent	400:410	arg1	nanoprobe					412:420	a novel fluorescent nanoprobe	392:420	a novel fluorescent nanoprobe for accurate SLN mapping	392:445	The current study designed a novel fluorescent nanoprobe for accurate SLN mapping.
24957293	0	49	theme	sentinel	41:48	arg1	node					56:59	sentinel lymph node	41:59	sentinel lymph node mapping	41:67	Dextran-based fluorescent nanoprobes for sentinel lymph node mapping.
24957293	3	50	theme	current	369:375	arg1	study					377:381	The current study	365:381	The current study	365:381	The current study designed a novel fluorescent nanoprobe for accurate SLN mapping.
24957293	1	51	theme	proper	188:193	arg1	positioning					199:209	proper SLN positioning	188:209	proper SLN positioning	188:209	Biopsy of sentinel lymph node (SLN) has become a common practice to predict whether tumor metastasis has occurred, so proper SLN positioning tracers are highly required.
24957293	5	52	theme	specific	765:772	arg1	imaging					778:784	specific SLN imaging	765:784	specific SLN imaging	765:784	The newly fabricated fluorescent DNG (FDNG) could be designed with optimum size and stable fluorescent intensity for specific SLN imaging.
24957293	7	53	theme	in vivo	975:981	arg1	evaluations					983:993	both in vitro and in vivo evaluations	957:993	evaluations	983:993	Most importantly, both in vitro and in vivo evaluations indicated that FDNG had fine biosafety and biocompatibility with lymphatic endothelial cells.
24957293	7	54	theme	fine	1019:1022	arg1	biosafety					1024:1032	fine biosafety	1019:1032	fine biosafety	1019:1032	Most importantly, both in vitro and in vivo evaluations indicated that FDNG had fine biosafety and biocompatibility with lymphatic endothelial cells.
24957293	0	55	theme	node	56:59	arg1	mapping					61:67	sentinel lymph node mapping	41:67	sentinel lymph node mapping	41:67	Dextran-based fluorescent nanoprobes for sentinel lymph node mapping.
24957293	5	56	theme	fabricated	658:667	arg1	FDNG					686:689	FDNG	686:689	FDNG	686:689	The newly fabricated fluorescent DNG (FDNG) could be designed with optimum size and stable fluorescent intensity for specific SLN imaging.
24957293	5	56	theme	fabricated	658:667	arg1	DNG					681:683	The newly fabricated fluorescent DNG	648:683	The newly fabricated fluorescent DNG (FDNG)	648:690	The newly fabricated fluorescent DNG (FDNG) could be designed with optimum size and stable fluorescent intensity for specific SLN imaging.
24957293	9	57	theme	various	1563:1569	arg1	diseases					1588:1595	various lymph-associated diseases	1563:1595	various lymph-associated diseases	1563:1595	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	2	58	theme	ideal	304:308	arg1	agents					331:336	ideal, biosafe SLN imaging agents	304:336	ideal, biosafe SLN imaging agents	304:336	Due to many drawbacks of SLN tracers currently used, developing ideal, biosafe SLN imaging agents is always an urgent issue.
24957293	5	59	theme	fluorescent	669:679	arg1	FDNG					686:689	FDNG	686:689	FDNG	686:689	The newly fabricated fluorescent DNG (FDNG) could be designed with optimum size and stable fluorescent intensity for specific SLN imaging.
24957293	5	59	theme	fluorescent	669:679	arg1	DNG					681:683	The newly fabricated fluorescent DNG	648:683	The newly fabricated fluorescent DNG (FDNG)	648:690	The newly fabricated fluorescent DNG (FDNG) could be designed with optimum size and stable fluorescent intensity for specific SLN imaging.
24957293	6	60	theme	dynamic	812:818	arg1	course					820:825	a long-term dynamic course	800:825	a long-term dynamic course in vivo	800:833	Furthermore, a long-term dynamic course in vivo (from 1 min to 72 h) revealed the satisfactory specificity, sensitivity, and stability for SLN mapping.
24957293	9	61	theme	lymph-associated	1571:1586	arg1	diseases					1588:1595	various lymph-associated diseases	1563:1595	various lymph-associated diseases	1563:1595	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	7	62	theme	in vitro	962:969	arg1	evaluations					983:993	both in vitro and in vivo evaluations	957:993	evaluations	983:993	Most importantly, both in vitro and in vivo evaluations indicated that FDNG had fine biosafety and biocompatibility with lymphatic endothelial cells.
24957293	3	63	theme	SLN	435:437	arg1	mapping					439:445	accurate SLN mapping	426:445	accurate SLN mapping	426:445	The current study designed a novel fluorescent nanoprobe for accurate SLN mapping.
24957293	8	64	theme	tumor	1335:1339	arg1	metastasis					1341:1350	tumor metastasis	1335:1350	tumor metastasis	1335:1350	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	1	65	theme	SLN	195:197	arg1	positioning					199:209	proper SLN positioning	188:209	proper SLN positioning	188:209	Biopsy of sentinel lymph node (SLN) has become a common practice to predict whether tumor metastasis has occurred, so proper SLN positioning tracers are highly required.
24957293	8	66	theme	SLN	1306:1308	arg1	biopsy					1310:1315	SLN biopsy	1306:1315	SLN biopsy and thus predicts tumor metastasis as well as directs therapies	1306:1379	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	6	67	theme	long-term	802:810	arg1	course					820:825	a long-term dynamic course	800:825	a long-term dynamic course in vivo	800:833	Furthermore, a long-term dynamic course in vivo (from 1 min to 72 h) revealed the satisfactory specificity, sensitivity, and stability for SLN mapping.
24957293	8	68	theme	efficient	1270:1278	arg1	location					1293:1300	efficient and accurate location	1270:1300	efficient and accurate location	1270:1300	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	9	69	theme	specific	1462:1469	arg1	carrier					1493:1499	a specific and controllable drug carrier	1460:1499	a specific and controllable drug carrier	1460:1499	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	9	69	theme	specific	1462:1469	arg1	DNG					1436:1438	DNG	1436:1438	DNG	1436:1438	Besides, our recent studies further demonstrated that DNG could also serve as a specific and controllable drug carrier, indicating a potential application for specific therapies of various lymph-associated diseases.
24957293	8	70	dep	biopsy	1310:1315	arg1	predicts					1326:1333	predicts	1326:1333	and thus predicts tumor metastasis as well as directs therapies	1317:1379	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	8	70	dep	biopsy	1310:1315	arg1	directs					1363:1369	directs	1363:1369	and thus predicts tumor metastasis as well as directs therapies	1317:1379	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	1	71	theme	common	119:124	arg1	practice					126:133	a common practice	117:133	a common practice to predict whether tumor metastasis has occurred, so proper SLN positioning tracers are highly required	117:237	Biopsy of sentinel lymph node (SLN) has become a common practice to predict whether tumor metastasis has occurred, so proper SLN positioning tracers are highly required.
24957293	3	72	theme	novel	394:398	arg1	nanoprobe					412:420	a novel fluorescent nanoprobe	392:420	a novel fluorescent nanoprobe for accurate SLN mapping	392:445	The current study designed a novel fluorescent nanoprobe for accurate SLN mapping.
24957293	8	73	theme	sensitive	1200:1208	arg1	mapping					1246:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	specific, sensitive, stable, non-invasive, and safe SLN mapping	1190:1252	All these results supported that FDNG could be used as highly efficient molecular imaging probes for specific, sensitive, stable, non-invasive, and safe SLN mapping, which provides efficient and accurate location for SLN biopsy and thus predicts tumor metastasis as well as directs therapies.
24957293	2	74	theme	imaging	323:329	arg1	agents					331:336	ideal, biosafe SLN imaging agents	304:336	ideal, biosafe SLN imaging agents	304:336	Due to many drawbacks of SLN tracers currently used, developing ideal, biosafe SLN imaging agents is always an urgent issue.
24957293	6	75	dep	72 h	850:853	arg1	1 min					841:845	1 min	841:845	1 min	841:845	Furthermore, a long-term dynamic course in vivo (from 1 min to 72 h) revealed the satisfactory specificity, sensitivity, and stability for SLN mapping.
28192670	3	0	from	charges	692:698	arg1	surface					707:713	CNF surface	703:713	CNF surface	703:713	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	5	1	theme	pristine	1475:1482	arg1	gels					1484:1487	those pristine gels	1469:1487	those pristine gels	1469:1487	Moreover, the depth-sensing instrumentation by indentation test also demonstrates that the CNF ionic coordination contributes simultaneous improvement in hardness and elasticity by as much as 600% compared to those pristine gels.
28192670	1	2	theme	biological	286:295	arg1	materials					297:305	mechanically superior biological materials	264:305	mechanically superior biological materials	264:305	Utilization of reversible interactions as sacrificial bonds in biopolymers is critical for the integral synthesis of mechanically superior biological materials.
28192670	3	3	theme	mechanical	732:741	arg1	properties					743:752	mechanical properties	732:752	mechanical properties	732:752	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	6	4	theme	dynamic	1596:1602	arg1	properties					1604:1613	tunable dynamic properties	1588:1613	tunable dynamic properties	1588:1613	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	6	5	theme	bulk	1567:1570	arg1	elastomers					1572:1581	bulk elastomers	1567:1581	bulk elastomers with tunable dynamic properties	1567:1613	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	6	6	with	elastomers	1572:1581	arg1	properties					1604:1613	tunable dynamic properties	1588:1613	tunable dynamic properties	1588:1613	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	2	7	theme	cellulose	322:330	arg1	nanofibrils					332:342	cellulose nanofibrils	322:342	cellulose nanofibrils (CNFs)	322:349	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	2	7	theme	cellulose	322:330	arg1	CNFs					345:348	CNFs	345:348	CNFs	345:348	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	4	8	theme	multiple	1121:1128	arg1	mechanisms					1141:1150	the multiple toughening mechanisms	1117:1150	the multiple toughening mechanisms	1117:1150	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	3	9	with	hydrogels	844:852	arg1	kJ/m3					936:940	fracture toughness 386 kJ/m3	913:940	fracture toughness 386 kJ/m3	913:940	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	9	with	hydrogels	844:852	arg1	properties					901:910	tough properties	895:910	tough properties (fracture toughness 386 kJ/m3)	895:941	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	9	with	hydrogels	844:852	arg1	stiff					859:863	stiff	859:863	stiff (Young's modulus 257 kPa)	859:889	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	9	with	hydrogels	844:852	arg1	kPa					886:888	Young's modulus 257 kPa	866:888	Young's modulus 257 kPa	866:888	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	6	10	theme	new	1633:1635	arg1	prospect					1637:1644	a new prospect	1631:1644	a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed	1631:1753	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	3	11	theme	modulus	874:880	arg1	stiff					859:863	stiff	859:863	stiff (Young's modulus 257 kPa)	859:889	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	11	theme	modulus	874:880	arg1	kPa					886:888	Young's modulus 257 kPa	866:888	Young's modulus 257 kPa	866:888	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	2	12	theme	cross-linked	564:575	arg1	hydrogels					577:585	the ionic-covalent cross-linked hydrogels	545:585	the ionic-covalent cross-linked hydrogels	545:585	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	6	13	theme	high-bearing	1721:1732	arg1	capability					1734:1743	high-bearing capability	1721:1743	high-bearing capability	1721:1743	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	5	14	from	improvement	1399:1409	arg1	elasticity					1427:1436	elasticity	1427:1436	elasticity	1427:1436	Moreover, the depth-sensing instrumentation by indentation test also demonstrates that the CNF ionic coordination contributes simultaneous improvement in hardness and elasticity by as much as 600% compared to those pristine gels.
28192670	5	14	from	improvement	1399:1409	arg1	hardness					1414:1421	hardness	1414:1421	hardness	1414:1421	Moreover, the depth-sensing instrumentation by indentation test also demonstrates that the CNF ionic coordination contributes simultaneous improvement in hardness and elasticity by as much as 600% compared to those pristine gels.
28192670	4	15	theme	synergistic	1208:1218	arg1	combination					1220:1230	synergistic combination	1208:1230	synergistic combination of stiffness and toughness	1208:1257	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	3	16	theme	reinforced	827:836	arg1	hydrogels					844:852	mechanically reinforced ionic hydrogels	814:852	mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3)	814:941	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	17	theme	networks	632:639	arg1	formation					612:620	the formation	608:620	the formation of porous networks of nanofibrils	608:654	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	18	theme	Young	866:870	arg1	stiff					859:863	stiff	859:863	stiff (Young's modulus 257 kPa)	859:889	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	18	theme	Young	866:870	arg1	kPa					886:888	Young's modulus 257 kPa	866:888	Young's modulus 257 kPa	866:888	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	4	19	theme	balanced	1163:1170	arg1	manner					1191:1196	a balanced energy dissipation manner	1161:1196	a balanced energy dissipation manner	1161:1196	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	1	20	theme	reversible	162:171	arg1	interactions					173:184	reversible interactions	162:184	reversible interactions	162:184	Utilization of reversible interactions as sacrificial bonds in biopolymers is critical for the integral synthesis of mechanically superior biological materials.
28192670	4	21	theme	dissipation	1179:1189	arg1	manner					1191:1196	a balanced energy dissipation manner	1161:1196	a balanced energy dissipation manner	1161:1196	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	4	22	theme	in	948:949	arg1	spectroscopy					962:973	The in situ Raman spectroscopy	944:973	The in situ Raman spectroscopy during stretching	944:991	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	5	23	theme	indentation	1307:1317	arg1	test					1319:1322	indentation test	1307:1322	indentation test	1307:1322	Moreover, the depth-sensing instrumentation by indentation test also demonstrates that the CNF ionic coordination contributes simultaneous improvement in hardness and elasticity by as much as 600% compared to those pristine gels.
28192670	0	24	theme	Mechanistic	97:107	arg1	Insight					109:115	Mechanistic Insight	97:115	Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics	19:144	Metal Ion Mediated Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics.
28192670	6	25	theme	reinforced	1677:1686	arg1	hydrogels					1688:1696	CNF reinforced hydrogels	1673:1696	CNF reinforced hydrogels	1673:1696	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	3	26	theme	fracture	913:920	arg1	kJ/m3					936:940	fracture toughness 386 kJ/m3	913:940	fracture toughness 386 kJ/m3	913:940	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	26	theme	fracture	913:920	arg1	properties					901:910	tough properties	895:910	tough properties (fracture toughness 386 kJ/m3)	895:941	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	4	27	theme	Raman	956:960	arg1	spectroscopy					962:973	The in situ Raman spectroscopy	944:973	The in situ Raman spectroscopy during stretching	944:991	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	0	28	from	Network	51:57	arg1	Hydrogels					86:94	Covalently Cross-linked Hydrogels	62:94	Covalently Cross-linked Hydrogels	62:94	Metal Ion Mediated Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics.
28192670	5	29	theme	simultaneous	1386:1397	arg1	improvement					1399:1409	simultaneous improvement	1386:1409	simultaneous improvement in hardness and elasticity	1386:1436	Moreover, the depth-sensing instrumentation by indentation test also demonstrates that the CNF ionic coordination contributes simultaneous improvement in hardness and elasticity by as much as 600% compared to those pristine gels.
28192670	4	30	theme	CNF	1036:1038	arg1	medium					1026:1031	the stress transfer medium	1006:1031	the stress transfer medium of CNF	1006:1038	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	0	31	theme	Metal	0:4	arg1	Ion					6:8	Metal Ion	0:8	Metal Ion	0:8	Metal Ion Mediated Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics.
28192670	6	32	theme	rational	1654:1661	arg1	design					1663:1668	the rational design	1650:1668	the rational design of CNF reinforced hydrogels	1650:1696	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	5	33	theme	CNF	1351:1353	arg1	coordination					1361:1372	the CNF ionic coordination	1347:1372	the CNF ionic coordination	1347:1372	Moreover, the depth-sensing instrumentation by indentation test also demonstrates that the CNF ionic coordination contributes simultaneous improvement in hardness and elasticity by as much as 600% compared to those pristine gels.
28192670	4	34	theme	stress	1010:1015	arg1	medium					1026:1031	the stress transfer medium	1006:1031	the stress transfer medium of CNF	1006:1038	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	2	35	theme	cation	443:448	arg1	solution					487:494	multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution	431:494	multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution	431:494	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	3	36	theme	nanofibrils	644:654	arg1	networks					632:639	porous networks	625:639	porous networks of nanofibrils	625:654	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	2	37	theme	polyacrylamide	371:384	arg1	hydrogels					403:411	covalent polyacrylamide (PAAm) composite hydrogels	362:411	covalent polyacrylamide (PAAm) composite hydrogels	362:411	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	0	38	dep	Nanofibrils	29:39	arg1	Insight					109:115	Mechanistic Insight	97:115	Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics	19:144	Metal Ion Mediated Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics.
28192670	0	38	dep	Nanofibrils	29:39	arg1	Network					51:57	Transient Network	41:57	Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics	19:144	Metal Ion Mediated Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics.
28192670	0	39	link	Cross-linked	73:84	arg1	Hydrogels					86:94	Covalently Cross-linked Hydrogels	62:94	Covalently Cross-linked Hydrogels	62:94	Metal Ion Mediated Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics.
28192670	4	40	theme	crack	1084:1088	arg1	propagation					1090:1100	stable crack propagation	1077:1100	stable crack propagation	1077:1100	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	1	41	theme	integral	242:249	arg1	synthesis					251:259	the integral synthesis	238:259	the integral synthesis of mechanically superior biological materials	238:305	Utilization of reversible interactions as sacrificial bonds in biopolymers is critical for the integral synthesis of mechanically superior biological materials.
28192670	3	42	theme	repulsive	673:681	arg1	charges					692:698	the repulsive negative charges	669:698	the repulsive negative charges on CNF surface	669:713	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	2	43	theme	composite	393:401	arg1	hydrogels					403:411	covalent polyacrylamide (PAAm) composite hydrogels	362:411	covalent polyacrylamide (PAAm) composite hydrogels	362:411	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	4	44	theme	microscopic	1049:1059	arg1	morphologies					1061:1072	the microscopic morphologies	1045:1072	the microscopic morphologies of stable crack propagation	1045:1100	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	2	45	theme	PAAm	387:390	arg1	hydrogels					403:411	covalent polyacrylamide (PAAm) composite hydrogels	362:411	covalent polyacrylamide (PAAm) composite hydrogels	362:411	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	1	46	theme	superior	277:284	arg1	materials					297:305	mechanically superior biological materials	264:305	mechanically superior biological materials	264:305	Utilization of reversible interactions as sacrificial bonds in biopolymers is critical for the integral synthesis of mechanically superior biological materials.
28192670	3	47	theme	CNF	703:705	arg1	surface					707:713	CNF surface	703:713	CNF surface	703:713	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	1	48	theme	materials	297:305	arg1	synthesis					251:259	the integral synthesis	238:259	the integral synthesis of mechanically superior biological materials	238:305	Utilization of reversible interactions as sacrificial bonds in biopolymers is critical for the integral synthesis of mechanically superior biological materials.
28192670	6	49	theme	tunable	1588:1594	arg1	properties					1604:1613	tunable dynamic properties	1588:1613	tunable dynamic properties	1588:1613	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	1	50	theme	sacrificial	189:199	arg1	bonds					201:205	sacrificial bonds	189:205	sacrificial bonds in biopolymers	189:220	Utilization of reversible interactions as sacrificial bonds in biopolymers is critical for the integral synthesis of mechanically superior biological materials.
28192670	2	51	link	cross-linked	564:575	arg1	hydrogels					577:585	the ionic-covalent cross-linked hydrogels	545:585	the ionic-covalent cross-linked hydrogels	545:585	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	4	52	theme	toughening	1130:1139	arg1	mechanisms					1141:1150	the multiple toughening mechanisms	1117:1150	the multiple toughening mechanisms	1117:1150	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	3	53	theme	self-recovery	758:770	arg1	efficiency					772:781	self-recovery efficiency	758:781	self-recovery efficiency	758:781	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	4	54	dep	in	948:949	arg1	situ					951:954	situ	951:954	situ	951:954	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	6	55	theme	building	1548:1555	arg1	blocks					1557:1562	nanoscale building blocks	1538:1562	nanoscale building blocks	1538:1562	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	3	56	theme	hydrogels	790:798	arg1	efficiency					772:781	self-recovery efficiency	758:781	self-recovery efficiency	758:781	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	56	theme	hydrogels	790:798	arg1	properties					743:752	mechanical properties	732:752	mechanical properties	732:752	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	57	theme	porous	625:630	arg1	networks					632:639	porous networks	625:639	porous networks of nanofibrils	625:654	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	2	58	theme	ionic-covalent	549:562	arg1	hydrogels					577:585	the ionic-covalent cross-linked hydrogels	545:585	the ionic-covalent cross-linked hydrogels	545:585	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	4	59	theme	energy	1172:1177	arg1	manner					1191:1196	a balanced energy dissipation manner	1161:1196	a balanced energy dissipation manner	1161:1196	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	3	60	theme	ionic	838:842	arg1	hydrogels					844:852	mechanically reinforced ionic hydrogels	814:852	mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3)	814:941	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	0	61	theme	Cross-linked	73:84	arg1	Hydrogels					86:94	Covalently Cross-linked Hydrogels	62:94	Covalently Cross-linked Hydrogels	62:94	Metal Ion Mediated Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics.
28192670	5	62	theme	depth-sensing	1274:1286	arg1	instrumentation					1288:1302	the depth-sensing instrumentation	1270:1302	the depth-sensing instrumentation by indentation test	1270:1322	Moreover, the depth-sensing instrumentation by indentation test also demonstrates that the CNF ionic coordination contributes simultaneous improvement in hardness and elasticity by as much as 600% compared to those pristine gels.
28192670	1	63	from	bonds	201:205	arg1	biopolymers					210:220	biopolymers	210:220	biopolymers	210:220	Utilization of reversible interactions as sacrificial bonds in biopolymers is critical for the integral synthesis of mechanically superior biological materials.
28192670	1	64	theme	interactions	173:184	arg1	Utilization					147:157	Utilization	147:157	Utilization of reversible interactions as sacrificial bonds in biopolymers	147:220	Utilization of reversible interactions as sacrificial bonds in biopolymers is critical for the integral synthesis of mechanically superior biological materials.
28192670	3	65	theme	tough	895:899	arg1	kJ/m3					936:940	fracture toughness 386 kJ/m3	913:940	fracture toughness 386 kJ/m3	913:940	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	65	theme	tough	895:899	arg1	properties					901:910	tough properties	895:910	tough properties (fracture toughness 386 kJ/m3)	895:941	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	2	66	theme	aqueous	479:485	arg1	solution					487:494	multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution	431:494	multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution	431:494	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	6	67	theme	hydrogels	1688:1696	arg1	design					1663:1668	the rational design	1650:1668	the rational design of CNF reinforced hydrogels	1650:1696	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	6	68	theme	CNF	1673:1675	arg1	hydrogels					1688:1696	CNF reinforced hydrogels	1673:1696	CNF reinforced hydrogels	1673:1696	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	3	69	theme	toughness	922:930	arg1	kJ/m3					936:940	fracture toughness 386 kJ/m3	913:940	fracture toughness 386 kJ/m3	913:940	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	3	69	theme	toughness	922:930	arg1	properties					901:910	tough properties	895:910	tough properties (fracture toughness 386 kJ/m3)	895:941	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	2	70	theme	ionic	504:508	arg1	association					510:520	ionic association	504:520	ionic association among CNFs	504:531	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	4	71	theme	stiffness	1235:1243	arg1	combination					1220:1230	synergistic combination	1208:1230	synergistic combination of stiffness and toughness	1208:1257	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	4	72	theme	toughness	1249:1257	arg1	combination					1220:1230	synergistic combination	1208:1230	synergistic combination of stiffness and toughness	1208:1257	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	0	73	theme	Cellulose	19:27	arg1	Nanofibrils					29:39	Cellulose Nanofibrils	19:39	Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics	19:144	Metal Ion Mediated Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics.
28192670	5	74	theme	ionic	1355:1359	arg1	coordination					1361:1372	the CNF ionic coordination	1347:1372	the CNF ionic coordination	1347:1372	Moreover, the depth-sensing instrumentation by indentation test also demonstrates that the CNF ionic coordination contributes simultaneous improvement in hardness and elasticity by as much as 600% compared to those pristine gels.
28192670	0	75	theme	Transient	41:49	arg1	Network					51:57	Transient Network	41:57	Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics	19:144	Metal Ion Mediated Cellulose Nanofibrils Transient Network in Covalently Cross-linked Hydrogels: Mechanistic Insight into Morphology and Dynamics.
28192670	2	76	theme	multivalent	431:441	arg1	solution					487:494	multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution	431:494	multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution	431:494	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	2	77	dep	solution	487:494	arg1	Al3+					463:466	Al3+	463:466	Al3+	463:466	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	2	77	dep	solution	487:494	arg1	Ce3+					473:476	Ce3+	473:476	Ce3+	473:476	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	2	77	dep	solution	487:494	arg1	Zn2+					457:460	Zn2+	457:460	Zn2+	457:460	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	2	77	dep	solution	487:494	arg1	Ca2+					451:454	Ca2+	451:454	Ca2+	451:454	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	4	78	theme	transfer	1017:1024	arg1	medium					1026:1031	the stress transfer medium	1006:1031	the stress transfer medium of CNF	1006:1038	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	3	79	theme	negative	683:690	arg1	charges					692:698	the repulsive negative charges	669:698	the repulsive negative charges on CNF surface	669:713	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	2	80	theme	covalent	362:369	arg1	hydrogels					403:411	covalent polyacrylamide (PAAm) composite hydrogels	362:411	covalent polyacrylamide (PAAm) composite hydrogels	362:411	In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels.
28192670	6	81	theme	nanoscale	1538:1546	arg1	blocks					1557:1562	nanoscale building blocks	1538:1562	nanoscale building blocks	1538:1562	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
28192670	3	82	dep	properties	743:752	arg1	the					728:730	the	728:730	the	728:730	The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3).
28192670	4	83	theme	stable	1077:1082	arg1	propagation					1090:1100	stable crack propagation	1077:1100	stable crack propagation	1077:1100	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	4	84	theme	propagation	1090:1100	arg1	morphologies					1061:1072	the microscopic morphologies	1045:1072	the microscopic morphologies of stable crack propagation	1045:1100	The in situ Raman spectroscopy during stretching corroborates the stress transfer medium of CNF, and the microscopic morphologies of stable crack propagation validates that the multiple toughening mechanisms occur in a balanced energy dissipation manner, enabling synergistic combination of stiffness and toughness.
28192670	6	85	theme	facile	1515:1520	arg1	way					1522:1524	a facile way	1513:1524	a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties	1513:1613	This work demonstrates a facile way to transfer nanoscale building blocks to bulk elastomers with tunable dynamic properties and may provide a new prospect for the rational design of CNF reinforced hydrogels for applications where high-bearing capability is needed.
24368285	6	0	theme	Raman	1047:1051	arg1	detection					1053:1061	Raman detection	1047:1061	Raman detection	1047:1061	Furthermore, the gel could be cleaned with washing solution and recycling could be achieved for Raman detection.
24368285	0	1	theme	SERS	79:82	arg1	detection					84:92	SERS detection	79:92	SERS detection	79:92	Preparation of gold nanoparticles-agarose gel composite and its application in SERS detection.
24368285	5	2	from	loss	891:894	arg1	air					912:914	the air	908:914	the air	908:914	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.
24368285	0	3	from	Preparation	0:10	arg1	detection					84:92	SERS detection	79:92	SERS detection	79:92	Preparation of gold nanoparticles-agarose gel composite and its application in SERS detection.
24368285	2	4	theme	electron	312:319	arg1	SEM					333:335	SEM	333:335	SEM	333:335	Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy.
24368285	2	4	theme	electron	312:319	arg1	microscopy					321:330	scanning electron microscopy	303:330	scanning electron microscopy (SEM)	303:336	Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy.
24368285	3	5	theme	scattering	588:597	arg1	nanocomposites					537:550	the nanocomposites	533:550	the nanocomposites	533:550	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	5	theme	scattering	588:597	arg1	substrate					606:614	surface enhanced Raman scattering (SERS) substrate	565:614	surface enhanced Raman scattering (SERS) substrate	565:614	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	6	theme	enhanced	573:580	arg1	scattering					588:597	enhanced Raman scattering	573:597	surface enhanced Raman scattering (SERS) substrate	565:614	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	6	theme	enhanced	573:580	arg1	SERS					600:603	SERS	600:603	SERS	600:603	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	2	7	theme	scanning	303:310	arg1	SEM					333:335	SEM	333:335	SEM	333:335	Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy.
24368285	2	7	theme	scanning	303:310	arg1	microscopy					321:330	scanning electron microscopy	303:330	scanning electron microscopy (SEM)	303:336	Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy.
24368285	3	8	theme	gold	513:516	arg1	nanoparticles					518:530	the gold nanoparticles	509:530	the gold nanoparticles	509:530	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	5	9	theme	dynamic	826:832	arg1	effect					843:848	dynamic hot-spot effect	826:848	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air	803:914	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.
24368285	1	10	theme	preformed	178:186	arg1	gel					196:198	preformed agarose gel	178:198	preformed agarose gel	178:198	Agarose gel/gold nanoparticles hybrid was prepared by adding gold nanoparticles to preformed agarose gel.
24368285	1	11	theme	agarose	188:194	arg1	gel					196:198	preformed agarose gel	178:198	preformed agarose gel	178:198	Agarose gel/gold nanoparticles hybrid was prepared by adding gold nanoparticles to preformed agarose gel.
24368285	4	12	theme	gel	729:731	arg1	structure					704:712	the porous structure	693:712	the porous structure of the agarose gel	693:731	Results revealed that the porous structure of the agarose gel provided a good carrier for the enrichment of the gold nanoparticles.
24368285	3	13	theme	adjustable	452:461	arg1	LSPR					500:503	LSPR	500:503	LSPR	500:503	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	13	theme	adjustable	452:461	arg1	resonance					489:497	the adjustable localized surface plasmon resonance	448:497	the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles	448:530	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	1	14	theme	Agarose	95:101	arg1	hybrid					126:131	Agarose gel/gold nanoparticles hybrid	95:131	Agarose gel/gold nanoparticles hybrid	95:131	Agarose gel/gold nanoparticles hybrid was prepared by adding gold nanoparticles to preformed agarose gel.
24368285	5	15	theme	agarose	867:873	arg1	loss					891:894	the agarose gel contraction loss	863:894	the agarose gel contraction loss of water in the air	863:914	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.
24368285	0	16	theme	nanoparticles-agarose	20:40	arg1	composite					46:54	gold nanoparticles-agarose gel composite	15:54	gold nanoparticles-agarose gel composite	15:54	Preparation of gold nanoparticles-agarose gel composite and its application in SERS detection.
24368285	4	17	theme	agarose	721:727	arg1	gel					729:731	the agarose gel	717:731	the agarose gel	717:731	Results revealed that the porous structure of the agarose gel provided a good carrier for the enrichment of the gold nanoparticles.
24368285	1	18	theme	gel/gold	103:110	arg1	hybrid					126:131	Agarose gel/gold nanoparticles hybrid	95:131	Agarose gel/gold nanoparticles hybrid	95:131	Agarose gel/gold nanoparticles hybrid was prepared by adding gold nanoparticles to preformed agarose gel.
24368285	3	19	dep	adjustable	452:461	arg1	localized					463:471	localized	463:471	localized	463:471	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	0	20	theme	gold	15:18	arg1	composite					46:54	gold nanoparticles-agarose gel composite	15:54	gold nanoparticles-agarose gel composite	15:54	Preparation of gold nanoparticles-agarose gel composite and its application in SERS detection.
24368285	1	21	theme	nanoparticles	112:124	arg1	hybrid					126:131	Agarose gel/gold nanoparticles hybrid	95:131	Agarose gel/gold nanoparticles hybrid	95:131	Agarose gel/gold nanoparticles hybrid was prepared by adding gold nanoparticles to preformed agarose gel.
24368285	3	22	theme	swelling-contraction	392:411	arg1	characteristics					413:427	the swelling-contraction characteristics	388:427	the swelling-contraction characteristics of agarose gel	388:442	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	0	23	theme	composite	46:54	arg1	Preparation					0:10	Preparation	0:10	Preparation of gold nanoparticles-agarose gel composite	0:54	Preparation of gold nanoparticles-agarose gel composite and its application in SERS detection.
24368285	0	23	theme	composite	46:54	arg1	application					64:74	its application	60:74	its application in SERS detection	60:92	Preparation of gold nanoparticles-agarose gel composite and its application in SERS detection.
24368285	3	24	theme	nanoparticles	518:530	arg1	characteristics					413:427	the swelling-contraction characteristics	388:427	the swelling-contraction characteristics of agarose gel	388:442	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	24	theme	nanoparticles	518:530	arg1	LSPR					500:503	LSPR	500:503	LSPR	500:503	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	24	theme	nanoparticles	518:530	arg1	resonance					489:497	the adjustable localized surface plasmon resonance	448:497	the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles	448:530	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	2	25	theme	transmission	263:274	arg1	microscopy					285:294	transmission electron microscopy	263:294	transmission electron microscopy (TEM)	263:300	Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy.
24368285	2	25	theme	transmission	263:274	arg1	TEM					297:299	TEM	297:299	TEM	297:299	Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy.
24368285	5	26	theme	hot-spot	834:841	arg1	effect					843:848	dynamic hot-spot effect	826:848	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air	803:914	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.
24368285	0	27	theme	gel	42:44	arg1	composite					46:54	gold nanoparticles-agarose gel composite	15:54	gold nanoparticles-agarose gel composite	15:54	Preparation of gold nanoparticles-agarose gel composite and its application in SERS detection.
24368285	4	28	theme	good	744:747	arg1	carrier					749:755	a good carrier	742:755	a good carrier for the enrichment of the gold nanoparticles	742:800	Results revealed that the porous structure of the agarose gel provided a good carrier for the enrichment of the gold nanoparticles.
24368285	4	29	theme	nanoparticles	788:800	arg1	enrichment					765:774	the enrichment	761:774	the enrichment of the gold nanoparticles	761:800	Results revealed that the porous structure of the agarose gel provided a good carrier for the enrichment of the gold nanoparticles.
24368285	3	30	dep	molecules	643:651	arg1	1NAT					664:667	1NAT	664:667	1NAT	664:667	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	30	dep	molecules	643:651	arg1	MBA					659:661	MBA	659:661	MBA	659:661	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	30	dep	molecules	643:651	arg1	NBA					654:656	NBA	654:656	NBA	654:656	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	31	theme	agarose	432:438	arg1	gel					440:442	agarose gel	432:442	agarose gel	432:442	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	5	32	theme	gel	875:877	arg1	loss					891:894	the agarose gel contraction loss	863:894	the agarose gel contraction loss of water in the air	863:914	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.
24368285	5	33	theme	gold	807:810	arg1	nanoparticles					812:824	The gold nanoparticles	803:824	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air	803:914	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.
24368285	0	34	from	application	64:74	arg1	detection					84:92	SERS detection	79:92	SERS detection	79:92	Preparation of gold nanoparticles-agarose gel composite and its application in SERS detection.
24368285	2	35	theme	absorption	354:363	arg1	spectroscopy					365:376	UV-Vis-NIR absorption spectroscopy	343:376	UV-Vis-NIR absorption spectroscopy	343:376	Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy.
24368285	3	36	theme	gel	440:442	arg1	characteristics					413:427	the swelling-contraction characteristics	388:427	the swelling-contraction characteristics of agarose gel	388:442	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	36	theme	gel	440:442	arg1	LSPR					500:503	LSPR	500:503	LSPR	500:503	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	36	theme	gel	440:442	arg1	resonance					489:497	the adjustable localized surface plasmon resonance	448:497	the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles	448:530	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	5	37	dep	nanoparticles	812:824	arg1	effect					843:848	dynamic hot-spot effect	826:848	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air	803:914	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.
24368285	2	38	theme	UV-Vis-NIR	343:352	arg1	spectroscopy					365:376	UV-Vis-NIR absorption spectroscopy	343:376	UV-Vis-NIR absorption spectroscopy	343:376	Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy.
24368285	3	39	theme	surface	473:479	arg1	LSPR					500:503	LSPR	500:503	LSPR	500:503	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	39	theme	surface	473:479	arg1	resonance					489:497	the adjustable localized surface plasmon resonance	448:497	the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles	448:530	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	2	40	theme	electron	276:283	arg1	microscopy					285:294	transmission electron microscopy	263:294	transmission electron microscopy (TEM)	263:300	Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy.
24368285	2	40	theme	electron	276:283	arg1	TEM					297:299	TEM	297:299	TEM	297:299	Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy.
24368285	3	41	theme	signal	636:641	arg1	molecules					643:651	the Raman signal molecules	626:651	the Raman signal molecules (NBA, MBA, 1NAT)	626:668	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	42	theme	Raman	582:586	arg1	scattering					588:597	enhanced Raman scattering	573:597	surface enhanced Raman scattering (SERS) substrate	565:614	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	42	theme	Raman	582:586	arg1	SERS					600:603	SERS	600:603	SERS	600:603	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	5	43	theme	water	899:903	arg1	loss					891:894	the agarose gel contraction loss	863:894	the agarose gel contraction loss of water in the air	863:914	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.
24368285	3	44	theme	surface	565:571	arg1	nanocomposites					537:550	the nanocomposites	533:550	the nanocomposites	533:550	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	44	theme	surface	565:571	arg1	substrate					606:614	surface enhanced Raman scattering (SERS) substrate	565:614	surface enhanced Raman scattering (SERS) substrate	565:614	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	5	45	theme	contraction	879:889	arg1	loss					891:894	the agarose gel contraction loss	863:894	the agarose gel contraction loss of water in the air	863:914	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.
24368285	3	46	theme	plasmon	481:487	arg1	LSPR					500:503	LSPR	500:503	LSPR	500:503	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	46	theme	plasmon	481:487	arg1	resonance					489:497	the adjustable localized surface plasmon resonance	448:497	the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles	448:530	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	2	47	theme	Nanocomposite	201:213	arg1	structures					215:224	Nanocomposite structures	201:224	Nanocomposite structures	201:224	Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy.
24368285	4	48	theme	gold	783:786	arg1	nanoparticles					788:800	the gold nanoparticles	779:800	the gold nanoparticles	779:800	Results revealed that the porous structure of the agarose gel provided a good carrier for the enrichment of the gold nanoparticles.
24368285	5	49	theme	Raman	937:941	arg1	signal					943:948	the Raman signal	933:948	the Raman signal	933:948	The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.
24368285	3	50	used	used	557:560	arg2	nanocomposites					537:550	the nanocomposites	533:550	the nanocomposites	533:550	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	50	used	used	557:560	arg2	substrate					606:614	surface enhanced Raman scattering (SERS) substrate	565:614	surface enhanced Raman scattering (SERS) substrate	565:614	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	3	51	theme	Raman	630:634	arg1	molecules					643:651	the Raman signal molecules	626:651	the Raman signal molecules (NBA, MBA, 1NAT)	626:668	Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules (NBA, MBA, 1NAT).
24368285	1	52	theme	gold	156:159	arg1	nanoparticles					161:173	gold nanoparticles	156:173	gold nanoparticles	156:173	Agarose gel/gold nanoparticles hybrid was prepared by adding gold nanoparticles to preformed agarose gel.
24368285	4	53	theme	porous	697:702	arg1	structure					704:712	the porous structure	693:712	the porous structure of the agarose gel	693:731	Results revealed that the porous structure of the agarose gel provided a good carrier for the enrichment of the gold nanoparticles.
24815988	4	0	theme	adapted	793:799	arg1	Fourier					801:807	a specially adapted Fourier	781:807	a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method	781:873	Epimerization was also monitored by polarimetry and infrared spectroscopy using a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method.
24815988	6	1	theme	order	1054:1058	arg1	kinetics					1071:1078	first order reversible kinetics	1048:1078	first order reversible kinetics between 25 to 60 °C	1048:1098	The three different analytical approaches unambiguously showed that the epimerization of lactose in aqueous solution follows first order reversible kinetics between 25 to 60 °C.
24815988	11	2	theme	aqueous	1758:1764	arg1	solutions					1766:1774	aqueous solutions	1758:1774	aqueous solutions of lactose	1758:1785	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	11	3	dep	significantly	1918:1930	arg1	%					1947:1947	greater than 1%	1933:1947	greater than 1%	1933:1947	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	6	4	from	epimerization	995:1007	arg1	solution					1031:1038	aqueous solution	1023:1038	aqueous solution	1023:1038	The three different analytical approaches unambiguously showed that the epimerization of lactose in aqueous solution follows first order reversible kinetics between 25 to 60 °C.
24815988	12	5	theme	equilibration	2235:2247	arg1	time					2249:2252	the required equilibration time	2222:2252	the required equilibration time	2222:2252	In contrast a controlled anomeric composition (~38% α and ~62% β) will be achieved if an aqueous solution is left to equilibrate for over 4 h at 25 °C, while increasing the temperature up to 60 °C rapidly reduces the required equilibration time.
24815988	2	6	theme	aqueous	266:272	arg1	solutions					274:282	the aqueous solutions	262:282	the aqueous solutions	262:282	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	1	7	theme	stability	162:170	arg1	aspects					138:144	the stereochemical aspects	119:144	the stereochemical aspects of the chemical stability of lactose solutions stored between 25 and 60 °C	119:219	This article reports on the stereochemical aspects of the chemical stability of lactose solutions stored between 25 and 60 °C.
24815988	9	8	theme	lactose	1518:1524	arg1	epimerization					1526:1538	lactose epimerization	1518:1538	lactose epimerization	1518:1538	The rate of epimerization for lactose increased with temperature and an Arrhenius plot yielded an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures.
24815988	10	9	theme	main	1617:1620	arg1	epimerization					1663:1675	epimerization	1663:1675	epimerization	1663:1675	The main mechanism affecting lactose stability is epimerization, as no permanent hydrolysis or chemical degradation was observed.
24815988	10	9	theme	main	1617:1620	arg1	mechanism					1622:1630	The main mechanism	1613:1630	The main mechanism affecting lactose stability	1613:1658	The main mechanism affecting lactose stability is epimerization, as no permanent hydrolysis or chemical degradation was observed.
24815988	1	10	theme	lactose	175:181	arg1	solutions					183:191	lactose solutions	175:191	lactose solutions stored between 25 and 60 °C	175:219	This article reports on the stereochemical aspects of the chemical stability of lactose solutions stored between 25 and 60 °C.
24815988	11	11	theme	analysis	1871:1878	arg1	time					1880:1883	analysis time	1871:1883	analysis time	1871:1883	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	8	12	theme	equilibrium	1288:1298	arg1	constant					1300:1307	an equilibrium constant	1285:1307	an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C	1285:1335	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	7	13	theme	rate	1113:1116	arg1	constant					1118:1125	The overall rate constant	1101:1125	The overall rate constant	1101:1125	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	7	13	theme	rate	1113:1116	arg1	±					1150:1150	4.4 × 10(-4) s(-1) ± 0.9	1131:1154	4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD))	1131:1182	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	4	14	theme	attenuated	828:837	arg1	method					868:873	attenuated total reflectance (FTIR-ATR) method	828:873	attenuated total reflectance (FTIR-ATR) method	828:873	Epimerization was also monitored by polarimetry and infrared spectroscopy using a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method.
24815988	2	15	theme	magnetic	474:481	arg1	NMR					494:496	NMR	494:496	NMR	494:496	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	2	15	theme	magnetic	474:481	arg1	resonance					483:491	nuclear magnetic resonance	466:491	nuclear magnetic resonance (NMR) analysis	466:506	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	3	16	theme	subsequent	673:682	arg1	analysis					684:691	subsequent analysis	673:691	subsequent analysis by NMR	673:698	Aliquots of lactose solutions were collected at different time points after the solutions were prepared and freeze-dried to remove water and halt epimerization for subsequent analysis by NMR.
24815988	11	17	theme	ice	1812:1814	arg1	bath					1816:1819	an ice bath	1809:1819	an ice bath at 0 °C	1809:1827	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	12	18	dep	°C	2203:2204	arg1	up					2194:2195	up	2194:2195	up	2194:2195	In contrast a controlled anomeric composition (~38% α and ~62% β) will be achieved if an aqueous solution is left to equilibrate for over 4 h at 25 °C, while increasing the temperature up to 60 °C rapidly reduces the required equilibration time.
24815988	4	19	theme	reflectance	845:855	arg1	method					868:873	attenuated total reflectance (FTIR-ATR) method	828:873	attenuated total reflectance (FTIR-ATR) method	828:873	Epimerization was also monitored by polarimetry and infrared spectroscopy using a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method.
24815988	8	20	theme	±	1316:1316	arg1	constant					1300:1307	an equilibrium constant	1285:1307	an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C	1285:1335	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	12	21	from	°C	2157:2158	arg1	h					2149:2149	4 h	2147:2149	4 h at 25 °C	2147:2158	In contrast a controlled anomeric composition (~38% α and ~62% β) will be achieved if an aqueous solution is left to equilibrate for over 4 h at 25 °C, while increasing the temperature up to 60 °C rapidly reduces the required equilibration time.
24815988	10	22	theme	permanent	1684:1692	arg1	hydrolysis					1694:1703	no permanent hydrolysis	1681:1703	no permanent hydrolysis	1681:1703	The main mechanism affecting lactose stability is epimerization, as no permanent hydrolysis or chemical degradation was observed.
24815988	9	23	theme	intermediate	1588:1599	arg1	structures					1601:1610	extremely short-lived intermediate structures	1566:1610	extremely short-lived intermediate structures	1566:1610	The rate of epimerization for lactose increased with temperature and an Arrhenius plot yielded an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures.
24815988	2	24	theme	nuclear	466:472	arg1	NMR					494:496	NMR	494:496	NMR	494:496	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	2	24	theme	nuclear	466:472	arg1	resonance					483:491	nuclear magnetic resonance	466:491	nuclear magnetic resonance (NMR) analysis	466:506	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	2	25	theme	GC	405:406	arg1	protocol					419:426	a GC analytical protocol	403:426	a GC analytical protocol	403:426	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	9	26	theme	short-lived	1576:1586	arg1	structures					1601:1610	extremely short-lived intermediate structures	1566:1610	extremely short-lived intermediate structures	1566:1610	The rate of epimerization for lactose increased with temperature and an Arrhenius plot yielded an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures.
24815988	0	27	theme	epimerization	51:63	arg1	kinetics					65:72	the epimerization kinetics	47:72	the epimerization kinetics of lactose in water	47:92	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.
24815988	6	28	theme	different	933:941	arg1	approaches					954:963	The three different analytical approaches	923:963	The three different analytical approaches	923:963	The three different analytical approaches unambiguously showed that the epimerization of lactose in aqueous solution follows first order reversible kinetics between 25 to 60 °C.
24815988	3	29	theme	solutions	529:537	arg1	Aliquots					509:516	Aliquots	509:516	Aliquots of lactose solutions	509:537	Aliquots of lactose solutions were collected at different time points after the solutions were prepared and freeze-dried to remove water and halt epimerization for subsequent analysis by NMR.
24815988	8	30	theme	rate	1206:1209	arg1	constant					1211:1218	The forward rate constant	1194:1218	The forward rate constant	1194:1218	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	8	30	theme	rate	1206:1209	arg1	times					1228:1232	times	1228:1232	times	1228:1232	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	5	31	theme	ion	903:905	arg1	chromatography					907:920	ion chromatography	903:920	ion chromatography	903:920	Hydrolysis was analyzed by ion chromatography.
24815988	2	32	theme	%	337:337	arg1	α					339:339	96% α	335:339	96% α	335:339	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	7	33	dep	±	1150:1150	arg1	SD					1179:1180	SD	1179:1180	SD	1179:1180	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	7	33	dep	±	1150:1150	arg1	deviation					1168:1176	± standard deviation	1157:1176	± standard deviation (SD)	1157:1181	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	11	34	theme	solid	1959:1963	arg1	composition					1971:1981	the solid state composition	1955:1981	the solid state composition of the starting material	1955:2006	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	12	35	theme	controlled	2023:2032	arg1	composition					2043:2053	a controlled anomeric composition	2021:2053	a controlled anomeric composition (~38% α and ~62% β)	2021:2073	In contrast a controlled anomeric composition (~38% α and ~62% β) will be achieved if an aqueous solution is left to equilibrate for over 4 h at 25 °C, while increasing the temperature up to 60 °C rapidly reduces the required equilibration time.
24815988	0	36	theme	sugar	13:17	arg1	solutions					19:27	sugar solutions	13:27	sugar solutions	13:27	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.
24815988	11	37	theme	material	1999:2006	arg1	composition					1971:1981	the solid state composition	1955:1981	the solid state composition of the starting material	1955:2006	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	11	38	from	°C	1826:1827	arg1	bath					1816:1819	an ice bath	1809:1819	an ice bath at 0 °C	1809:1827	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	11	38	from	°C	1826:1827	arg1	storage					1798:1804	immediate storage	1788:1804	immediate storage in an ice bath at 0 °C	1788:1827	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	3	39	theme	different	557:565	arg1	points					572:577	different time points	557:577	different time points after the solutions were prepared and freeze-dried to remove water and halt epimerization for subsequent analysis by NMR	557:698	Aliquots of lactose solutions were collected at different time points after the solutions were prepared and freeze-dried to remove water and halt epimerization for subsequent analysis by NMR.
24815988	8	40	theme	reverse	1251:1257	arg1	constant					1264:1271	the reverse rate constant	1247:1271	the reverse rate constant	1247:1271	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	2	41	theme	%	346:346	arg1	β					348:348	4% β	345:348	4% β	345:348	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	4	42	dep	Fourier	801:807	arg1	transform					809:817	transform	809:817	transform infrared attenuated total reflectance (FTIR-ATR) method	809:873	Epimerization was also monitored by polarimetry and infrared spectroscopy using a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method.
24815988	7	43	theme	overall	1105:1111	arg1	constant					1118:1125	The overall rate constant	1101:1125	The overall rate constant	1101:1125	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	7	43	theme	overall	1105:1111	arg1	±					1150:1150	4.4 × 10(-4) s(-1) ± 0.9	1131:1154	4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD))	1131:1182	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	2	44	theme	analysis	387:394	arg1	certificate					372:382	the supplied certificate	359:382	the supplied certificate	359:382	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	2	45	theme	analytical	408:417	arg1	protocol					419:426	a GC analytical protocol	403:426	a GC analytical protocol	403:426	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	0	46	theme	novel	32:36	arg1	study					38:42	a novel study	30:42	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.	0:93	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.
24815988	2	47	theme	α-lactose	288:296	arg1	monohydrate					298:308	α-lactose monohydrate	288:308	α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis	288:506	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	2	47	theme	α-lactose	288:296	arg1	lactose					226:232	The lactose	222:232	The lactose used for the preparation of the aqueous solutions	222:282	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	9	48	theme	energy	1447:1452	arg1	kJ/mol					1463:1468	an activation energy of +52.3 kJ/mol	1433:1468	an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures	1433:1610	The rate of epimerization for lactose increased with temperature and an Arrhenius plot yielded an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures.
24815988	7	49	theme	standard	1159:1166	arg1	SD					1179:1180	SD	1179:1180	SD	1179:1180	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	7	49	theme	standard	1159:1166	arg1	deviation					1168:1176	± standard deviation	1157:1176	± standard deviation (SD)	1157:1181	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	11	50	theme	immediate	1788:1796	arg1	storage					1798:1804	immediate storage	1788:1804	immediate storage in an ice bath at 0 °C	1788:1827	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	6	51	dep	60	1094:1095	arg1	to					1091:1092	to	1091:1092	to	1091:1092	The three different analytical approaches unambiguously showed that the epimerization of lactose in aqueous solution follows first order reversible kinetics between 25 to 60 °C.
24815988	0	52	dep	Stability	0:8	arg1	study					38:42	a novel study	30:42	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.	0:93	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.
24815988	6	53	theme	reversible	1060:1069	arg1	kinetics					1071:1078	first order reversible kinetics	1048:1078	first order reversible kinetics between 25 to 60 °C	1048:1098	The three different analytical approaches unambiguously showed that the epimerization of lactose in aqueous solution follows first order reversible kinetics between 25 to 60 °C.
24815988	8	54	from	constant	1300:1307	arg1	°C					1334:1335	25 °C	1331:1335	25 °C	1331:1335	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	2	55	theme	anomer	318:323	arg1	purity					325:330	an anomer purity	315:330	an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis	315:506	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	6	56	theme	first	1048:1052	arg1	kinetics					1071:1078	first order reversible kinetics	1048:1078	first order reversible kinetics between 25 to 60 °C	1048:1098	The three different analytical approaches unambiguously showed that the epimerization of lactose in aqueous solution follows first order reversible kinetics between 25 to 60 °C.
24815988	11	57	theme	anomeric	1896:1903	arg1	ratio					1905:1909	the anomeric ratio	1892:1909	the anomeric ratio	1892:1909	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	2	58	theme	solutions	274:282	arg1	preparation					247:257	the preparation	243:257	the preparation of the aqueous solutions	243:282	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	12	59	dep	composition	2043:2053	arg1	%					2059:2059	~38% α	2056:2061	~38% α	2056:2061	In contrast a controlled anomeric composition (~38% α and ~62% β) will be achieved if an aqueous solution is left to equilibrate for over 4 h at 25 °C, while increasing the temperature up to 60 °C rapidly reduces the required equilibration time.
24815988	12	59	dep	composition	2043:2053	arg1	%					2070:2070	~62% β	2067:2072	~62% β	2067:2072	In contrast a controlled anomeric composition (~38% α and ~62% β) will be achieved if an aqueous solution is left to equilibrate for over 4 h at 25 °C, while increasing the temperature up to 60 °C rapidly reduces the required equilibration time.
24815988	12	60	theme	required	2226:2233	arg1	time					2249:2252	the required equilibration time	2222:2252	the required equilibration time	2222:2252	In contrast a controlled anomeric composition (~38% α and ~62% β) will be achieved if an aqueous solution is left to equilibrate for over 4 h at 25 °C, while increasing the temperature up to 60 °C rapidly reduces the required equilibration time.
24815988	8	61	dep	times	1228:1232	arg1	greater					1234:1240	greater	1234:1240	greater	1234:1240	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	4	62	theme	infrared	753:760	arg1	spectroscopy					762:773	polarimetry and infrared spectroscopy	737:773	polarimetry and infrared spectroscopy using a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method	737:873	Epimerization was also monitored by polarimetry and infrared spectroscopy using a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method.
24815988	7	63	theme	s	1144:1144	arg1	constant					1118:1125	The overall rate constant	1101:1125	The overall rate constant	1101:1125	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	7	63	theme	s	1144:1144	arg1	±					1150:1150	4.4 × 10(-4) s(-1) ± 0.9	1131:1154	4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD))	1131:1182	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	10	64	theme	lactose	1642:1648	arg1	stability					1650:1658	lactose stability	1642:1658	lactose stability	1642:1658	The main mechanism affecting lactose stability is epimerization, as no permanent hydrolysis or chemical degradation was observed.
24815988	1	65	theme	chemical	153:160	arg1	stability					162:170	the chemical stability	149:170	the chemical stability of lactose solutions stored between 25 and 60 °C	149:219	This article reports on the stereochemical aspects of the chemical stability of lactose solutions stored between 25 and 60 °C.
24815988	4	66	theme	polarimetry	737:747	arg1	spectroscopy					762:773	polarimetry and infrared spectroscopy	737:773	polarimetry and infrared spectroscopy using a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method	737:873	Epimerization was also monitored by polarimetry and infrared spectroscopy using a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method.
24815988	9	67	theme	epimerization	1526:1538	arg1	mechanism					1505:1513	the mechanism	1501:1513	the mechanism of lactose epimerization	1501:1538	The rate of epimerization for lactose increased with temperature and an Arrhenius plot yielded an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures.
24815988	0	68	from	kinetics	65:72	arg1	water					88:92	water	88:92	water	88:92	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.
24815988	12	69	theme	aqueous	2098:2104	arg1	solution					2106:2113	an aqueous solution	2095:2113	an aqueous solution	2095:2113	In contrast a controlled anomeric composition (~38% α and ~62% β) will be achieved if an aqueous solution is left to equilibrate for over 4 h at 25 °C, while increasing the temperature up to 60 °C rapidly reduces the required equilibration time.
24815988	12	69	theme	aqueous	2098:2104	arg1	left					2118:2121	left	2118:2121	left	2118:2121	In contrast a controlled anomeric composition (~38% α and ~62% β) will be achieved if an aqueous solution is left to equilibrate for over 4 h at 25 °C, while increasing the temperature up to 60 °C rapidly reduces the required equilibration time.
24815988	1	70	theme	solutions	183:191	arg1	stability					162:170	the chemical stability	149:170	the chemical stability of lactose solutions stored between 25 and 60 °C	149:219	This article reports on the stereochemical aspects of the chemical stability of lactose solutions stored between 25 and 60 °C.
24815988	11	71	theme	time	1880:1883	arg1	min					1856:1858	approximately 3 min	1840:1858	approximately 3 min (180 s) of analysis time	1840:1883	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	11	71	theme	time	1880:1883	arg1	s					1865:1865	180 s	1861:1865	180 s	1861:1865	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	2	72	theme	resonance	483:491	arg1	analysis					499:506	nuclear magnetic resonance (NMR) analysis	466:506	nuclear magnetic resonance (NMR) analysis	466:506	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	0	73	from	water	88:92	arg1	study					38:42	a novel study	30:42	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.	0:93	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.
24815988	11	74	from	storage	1798:1804	arg1	bath					1816:1819	an ice bath	1809:1819	an ice bath at 0 °C	1809:1827	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	11	74	from	storage	1798:1804	arg1	°C					1826:1827	0 °C	1824:1827	0 °C	1824:1827	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	3	75	theme	lactose	521:527	arg1	solutions					529:537	lactose solutions	521:537	lactose solutions	521:537	Aliquots of lactose solutions were collected at different time points after the solutions were prepared and freeze-dried to remove water and halt epimerization for subsequent analysis by NMR.
24815988	4	76	theme	total	839:843	arg1	FTIR-ATR					858:865	FTIR-ATR	858:865	FTIR-ATR	858:865	Epimerization was also monitored by polarimetry and infrared spectroscopy using a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method.
24815988	4	76	theme	total	839:843	arg1	reflectance					845:855	total reflectance	839:855	attenuated total reflectance (FTIR-ATR) method	828:873	Epimerization was also monitored by polarimetry and infrared spectroscopy using a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method.
24815988	9	77	theme	structures	1601:1610	arg1	formation					1553:1561	the formation	1549:1561	the formation of extremely short-lived intermediate structures	1549:1610	The rate of epimerization for lactose increased with temperature and an Arrhenius plot yielded an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures.
24815988	0	78	theme	kinetics	65:72	arg1	study					38:42	a novel study	30:42	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.	0:93	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.
24815988	1	79	theme	stereochemical	123:136	arg1	aspects					138:144	the stereochemical aspects	119:144	the stereochemical aspects of the chemical stability of lactose solutions stored between 25 and 60 °C	119:219	This article reports on the stereochemical aspects of the chemical stability of lactose solutions stored between 25 and 60 °C.
24815988	6	80	theme	analytical	943:952	arg1	approaches					954:963	The three different analytical approaches	923:963	The three different analytical approaches	923:963	The three different analytical approaches unambiguously showed that the epimerization of lactose in aqueous solution follows first order reversible kinetics between 25 to 60 °C.
24815988	0	81	theme	lactose	77:83	arg1	kinetics					65:72	the epimerization kinetics	47:72	the epimerization kinetics of lactose in water	47:92	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.
24815988	9	82	theme	epimerization	1350:1362	arg1	rate					1342:1345	The rate	1338:1345	The rate of epimerization for lactose	1338:1374	The rate of epimerization for lactose increased with temperature and an Arrhenius plot yielded an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures.
24815988	0	83	from	study	38:42	arg1	water					88:92	water	88:92	water	88:92	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.
24815988	12	84	theme	anomeric	2034:2041	arg1	composition					2043:2053	a controlled anomeric composition	2021:2053	a controlled anomeric composition (~38% α and ~62% β)	2021:2073	In contrast a controlled anomeric composition (~38% α and ~62% β) will be achieved if an aqueous solution is left to equilibrate for over 4 h at 25 °C, while increasing the temperature up to 60 °C rapidly reduces the required equilibration time.
24815988	8	85	theme	forward	1198:1204	arg1	constant					1211:1218	The forward rate constant	1194:1218	The forward rate constant	1194:1218	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	8	85	theme	forward	1198:1204	arg1	times					1228:1232	times	1228:1232	times	1228:1232	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	7	86	theme	×	1135:1135	arg1	s					1144:1144	4.4 × 10(-4) s	1131:1144	4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD))	1131:1182	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	7	86	theme	×	1135:1135	arg1	-1					1146:1147	-1	1146:1147	-1	1146:1147	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	2	87	theme	α	339:339	arg1	purity					325:330	an anomer purity	315:330	an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis	315:506	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	8	88	from	°C	1334:1335	arg1	constant					1300:1307	an equilibrium constant	1285:1307	an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C	1285:1335	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	8	88	from	°C	1334:1335	arg1	±					1316:1316	1.6 ± 0.1 (±SD)	1312:1326	1.6 ± 0.1 (±SD) at 25 °C	1312:1335	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	6	89	theme	aqueous	1023:1029	arg1	solution					1031:1038	aqueous solution	1023:1038	aqueous solution	1023:1038	The three different analytical approaches unambiguously showed that the epimerization of lactose in aqueous solution follows first order reversible kinetics between 25 to 60 °C.
24815988	2	90	theme	supplied	363:370	arg1	certificate					372:382	the supplied certificate	359:382	the supplied certificate	359:382	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	8	91	theme	1.6	1224:1226	arg1	constant					1211:1218	The forward rate constant	1194:1218	The forward rate constant	1194:1218	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	8	91	theme	1.6	1224:1226	arg1	times					1228:1232	times	1228:1232	times	1228:1232	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	2	92	theme	96	335:336	arg1	%					337:337	%	337:337	%	337:337	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	0	93	theme	solutions	19:27	arg1	Stability					0:8	Stability	0:8	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.	0:93	Stability of sugar solutions: a novel study of the epimerization kinetics of lactose in water.
24815988	6	94	theme	lactose	1012:1018	arg1	epimerization					995:1007	the epimerization	991:1007	the epimerization of lactose in aqueous solution	991:1038	The three different analytical approaches unambiguously showed that the epimerization of lactose in aqueous solution follows first order reversible kinetics between 25 to 60 °C.
24815988	11	95	theme	state	1965:1969	arg1	composition					1971:1981	the solid state composition	1955:1981	the solid state composition of the starting material	1955:2006	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	2	96	theme	β	348:348	arg1	purity					325:330	an anomer purity	315:330	an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis	315:506	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	4	97	dep	transform	809:817	arg1	infrared					819:826	infrared	819:826	transform infrared attenuated total reflectance (FTIR-ATR) method	809:873	Epimerization was also monitored by polarimetry and infrared spectroscopy using a specially adapted Fourier transform infrared attenuated total reflectance (FTIR-ATR) method.
24815988	9	98	theme	Arrhenius	1410:1418	arg1	plot					1420:1423	an Arrhenius plot	1407:1423	an Arrhenius plot	1407:1423	The rate of epimerization for lactose increased with temperature and an Arrhenius plot yielded an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures.
24815988	10	99	theme	chemical	1708:1715	arg1	degradation					1717:1727	chemical degradation	1708:1727	chemical degradation	1708:1727	The main mechanism affecting lactose stability is epimerization, as no permanent hydrolysis or chemical degradation was observed.
24815988	2	100	theme	4	345:345	arg1	%					346:346	%	346:346	%	346:346	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	2	101	with	monohydrate	298:308	arg1	purity					325:330	an anomer purity	315:330	an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis	315:506	The lactose used for the preparation of the aqueous solutions was α-lactose monohydrate with an anomer purity of 96% α and 4% β based on the supplied certificate of analysis (using a GC analytical protocol), which was further confirmed here by nuclear magnetic resonance (NMR) analysis.
24815988	11	102	theme	starting	1990:1997	arg1	material					1999:2006	the starting material	1986:2006	the starting material	1986:2006	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
24815988	8	103	theme	rate	1259:1262	arg1	constant					1264:1271	the reverse rate constant	1247:1271	the reverse rate constant	1247:1271	The forward rate constant was 1.6 times greater than the reverse rate constant, leading to an equilibrium constant of 1.6 ± 0.1 (±SD) at 25 °C.
24815988	3	104	dep	water	640:644	arg1	epimerization					655:667	epimerization	655:667	epimerization	655:667	Aliquots of lactose solutions were collected at different time points after the solutions were prepared and freeze-dried to remove water and halt epimerization for subsequent analysis by NMR.
24815988	9	105	theme	activation	1436:1445	arg1	kJ/mol					1463:1468	an activation energy of +52.3 kJ/mol	1433:1468	an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures	1433:1610	The rate of epimerization for lactose increased with temperature and an Arrhenius plot yielded an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures.
24815988	3	106	theme	time	567:570	arg1	points					572:577	different time points	557:577	different time points after the solutions were prepared and freeze-dried to remove water and halt epimerization for subsequent analysis by NMR	557:698	Aliquots of lactose solutions were collected at different time points after the solutions were prepared and freeze-dried to remove water and halt epimerization for subsequent analysis by NMR.
24815988	9	107	theme	of	1454:1455	arg1	kJ/mol					1463:1468	an activation energy of +52.3 kJ/mol	1433:1468	an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures	1433:1610	The rate of epimerization for lactose increased with temperature and an Arrhenius plot yielded an activation energy of +52.3 kJ/mol supporting the hypothesis that the mechanism of lactose epimerization involves the formation of extremely short-lived intermediate structures.
24815988	7	108	from	°C	1190:1191	arg1	constant					1118:1125	The overall rate constant	1101:1125	The overall rate constant	1101:1125	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	7	108	from	°C	1190:1191	arg1	±					1150:1150	4.4 × 10(-4) s(-1) ± 0.9	1131:1154	4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD))	1131:1182	The overall rate constant was 4.4 × 10(-4) s(-1) ± 0.9 (± standard deviation (SD)) at 25 °C.
24815988	11	109	theme	lactose	1779:1785	arg1	solutions					1766:1774	aqueous solutions	1758:1774	aqueous solutions of lactose	1758:1785	When preparing aqueous solutions of lactose, immediate storage in an ice bath at 0 °C will allow approximately 3 min (180 s) of analysis time before the anomeric ratio alters significantly (greater than 1%) from the solid state composition of the starting material.
27490566	4	0	theme	individual	811:820	arg1	doses					822:826	individual doses	811:826	individual doses (GOS: 76-151 mg; inulin 114 mg)	811:858	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	6	1	theme	inulin	1136:1141	arg1	combination					1143:1153	inulin combination	1136:1153	inulin combination	1136:1153	It was observed that inhibition of ACF formation (55.8%) was significantly (p ≤ 0.05) higher using the GOS and inulin combination than GOS (41.4%) and inulin (51.2%) treatments alone.
27490566	7	2	theme	bacterial	1293:1301	arg1	activities					1313:1322	bacterial enzymatic activities	1293:1322	bacterial enzymatic activities	1293:1322	This combination also rendered better results on short-chain fatty acids (SCFA) and bacterial enzymatic activities.
27490566	7	3	theme	short-chain	1258:1268	arg1	SCFA					1283:1286	SCFA	1283:1286	SCFA	1283:1286	This combination also rendered better results on short-chain fatty acids (SCFA) and bacterial enzymatic activities.
27490566	7	3	theme	short-chain	1258:1268	arg1	acids					1276:1280	short-chain fatty acids	1258:1280	short-chain fatty acids (SCFA)	1258:1287	This combination also rendered better results on short-chain fatty acids (SCFA) and bacterial enzymatic activities.
27490566	3	4	theme	GOS	550:552	arg1	role					536:539	the role	532:539	the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model	532:735	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	9	5	theme	GOS	1497:1499	arg1	combination					1482:1492	novel combination	1476:1492	novel combination of GOS and inulin	1476:1510	Thus, this study demonstrated that novel combination of GOS and inulin exhibited stronger preventive activity than their individual treatments alone, and can be a promising strategy for CRC chemoprevention.
27490566	6	6	theme	formation	1064:1072	arg1	inhibition					1046:1055	inhibition	1046:1055	inhibition of ACF formation (55.8%)	1046:1080	It was observed that inhibition of ACF formation (55.8%) was significantly (p ≤ 0.05) higher using the GOS and inulin combination than GOS (41.4%) and inulin (51.2%) treatments alone.
27490566	6	7	dep	GOS	1160:1162	arg1	treatments					1191:1200	treatments	1191:1200	treatments	1191:1200	It was observed that inhibition of ACF formation (55.8%) was significantly (p ≤ 0.05) higher using the GOS and inulin combination than GOS (41.4%) and inulin (51.2%) treatments alone.
27490566	9	8	theme	inulin	1505:1510	arg1	combination					1482:1492	novel combination	1476:1492	novel combination of GOS and inulin	1476:1510	Thus, this study demonstrated that novel combination of GOS and inulin exhibited stronger preventive activity than their individual treatments alone, and can be a promising strategy for CRC chemoprevention.
27490566	3	9	theme	rodent	724:729	arg1	model					731:735	a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model	669:735	a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model	669:735	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	2	10	theme	prebiotic	326:334	arg1	GOS					362:364	GOS	362:364	GOS	362:364	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	10	theme	prebiotic	326:334	arg1	galacto-oligosaccharides					336:359	prebiotic galacto-oligosaccharides	326:359	prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention	326:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	4	11	theme	inulin	845:850	arg1	mg					856:857	inulin 114 mg	845:857	76-151 mg; inulin 114 mg	834:857	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	5	12	theme	GOS	966:968	arg1	effect					952:957	the significant preventive effect	925:957	the significant preventive effect of the GOS and inulin combination against the development of CRC	925:1022	Our data reveal the significant preventive effect of the GOS and inulin combination against the development of CRC.
27490566	3	13	theme	inulin	558:563	arg1	role					536:539	the role	532:539	the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model	532:735	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	4	14	dep	mg	790:791	arg1	each					793:796	each	793:796	each	793:796	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	0	15	from	Formation	98:106	arg1	Rats					149:152	Wistar Rats	142:152	Wistar Rats	142:152	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	5	16	theme	inulin	974:979	arg1	combination					981:991	inulin combination	974:991	inulin combination	974:991	Our data reveal the significant preventive effect of the GOS and inulin combination against the development of CRC.
27490566	3	17	theme	cancer	606:611	arg1	biomarkers					581:590	various biomarkers	573:590	various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF)	573:664	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	5	18	theme	preventive	941:950	arg1	effect					952:957	the significant preventive effect	925:957	the significant preventive effect of the GOS and inulin combination against the development of CRC	925:1022	Our data reveal the significant preventive effect of the GOS and inulin combination against the development of CRC.
27490566	6	19	dep	significantly	1086:1098	arg1	p					1101:1101	p ≤ 0.05	1101:1108	p ≤ 0.05	1101:1108	It was observed that inhibition of ACF formation (55.8%) was significantly (p ≤ 0.05) higher using the GOS and inulin combination than GOS (41.4%) and inulin (51.2%) treatments alone.
27490566	4	20	theme	DMH-treated	875:885	arg1	animals					887:893	DMH-treated animals	875:893	DMH-treated animals	875:893	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	0	21	from	Biomarkers	112:121	arg1	Rats					149:152	Wistar Rats	142:152	Wistar Rats	142:152	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	0	22	theme	Crypt	87:91	arg1	Formation					98:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	5	23	theme	CRC	1020:1022	arg1	development					1005:1015	the development	1001:1015	the development of CRC	1001:1022	Our data reveal the significant preventive effect of the GOS and inulin combination against the development of CRC.
27490566	4	24	dep	GOS	829:831	arg1	mg					841:842	76-151 mg	834:842	76-151 mg; inulin 114 mg	834:857	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	1	25	theme	beneficial	175:184	arg1	effects					186:192	beneficial effects	175:192	beneficial effects of prebiotics	175:206	The selectivity and beneficial effects of prebiotics are mainly dependent on composition and glycosidic linkage among monosaccharide units.
27490566	4	26	theme	Prebiotic	738:746	arg1	treatments					748:757	Prebiotic treatments	738:757	Prebiotic treatments of combined GOS and inulin (57 mg each)	738:797	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	2	27	contain	contains	372:379	arg2	combination					431:441	the novel combination	421:441	the novel combination of GOS and inulin in cancer prevention	421:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	27	contain	contains	372:379	arg1	GOS					362:364	GOS	362:364	GOS	362:364	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	27	contain	contains	372:379	arg2	linkages					408:415	β-1,6 and β-1,3 glycosidic linkages	381:415	β-1,6 and β-1,3 glycosidic linkages	381:415	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	27	contain	contains	372:379	arg1	galacto-oligosaccharides					336:359	prebiotic galacto-oligosaccharides	326:359	prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention	326:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	28	theme	first	307:311	arg1	study					313:317	the first study	303:317	the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention	303:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	28	theme	first	307:311	arg1	This					295:298	This	295:298	This	295:298	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	29	from	prevention	471:480	arg1	combination					431:441	the novel combination	421:441	the novel combination of GOS and inulin in cancer prevention	421:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	29	from	prevention	471:480	arg1	linkages					408:415	β-1,6 and β-1,3 glycosidic linkages	381:415	β-1,6 and β-1,3 glycosidic linkages	381:415	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	0	30	theme	Colon	126:130	arg1	Cancer					132:137	Colon Cancer	126:137	Colon Cancer	126:137	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	2	31	theme	cancer	464:469	arg1	prevention					471:480	cancer prevention	464:480	cancer prevention	464:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	0	32	theme	Foci	93:96	arg1	Formation					98:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	0	33	theme	Novel	0:4	arg1	Combination					6:16	Novel Combination	0:16	Novel Combination of Prebiotics Galacto-Oligosaccharides	0:55	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	2	34	theme	inulin	454:459	arg1	combination					431:441	the novel combination	421:441	the novel combination of GOS and inulin in cancer prevention	421:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	34	theme	inulin	454:459	arg1	linkages					408:415	β-1,6 and β-1,3 glycosidic linkages	381:415	β-1,6 and β-1,3 glycosidic linkages	381:415	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	35	theme	GOS	446:448	arg1	combination					431:441	the novel combination	421:441	the novel combination of GOS and inulin in cancer prevention	421:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	35	theme	GOS	446:448	arg1	linkages					408:415	β-1,6 and β-1,3 glycosidic linkages	381:415	β-1,6 and β-1,3 glycosidic linkages	381:415	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	3	36	theme	aberrant	640:647	arg1	foci					655:658	aberrant crypt foci	640:658	aberrant crypt foci (ACF)	640:664	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	3	36	theme	aberrant	640:647	arg1	ACF					661:663	ACF	661:663	ACF	661:663	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	0	37	theme	Galacto-Oligosaccharides	32:55	arg1	Formation					98:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	0	37	theme	Galacto-Oligosaccharides	32:55	arg1	Combination					6:16	Novel Combination	0:16	Novel Combination of Prebiotics Galacto-Oligosaccharides	0:55	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	0	37	theme	Galacto-Oligosaccharides	32:55	arg1	Biomarkers					112:121	Biomarkers	112:121	Biomarkers of Colon Cancer	112:137	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	4	38	dep	doses	822:826	arg1	GOS					829:831	GOS	829:831	GOS	829:831	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	8	39	located	observed	1382:1389	arg1	cecum					1394:1398	cecum	1394:1398	cecum	1394:1398	Dose-dependent effects of prebiotic treatments were also observed on cecum and fecal bacterial enzymes and on SCFA.
27490566	8	39	located	observed	1382:1389	arg2	effects					1340:1346	Dose-dependent effects	1325:1346	Dose-dependent effects of prebiotic treatments	1325:1370	Dose-dependent effects of prebiotic treatments were also observed on cecum and fecal bacterial enzymes and on SCFA.
27490566	8	40	theme	fecal	1404:1408	arg1	enzymes					1420:1426	fecal bacterial enzymes	1404:1426	fecal bacterial enzymes	1404:1426	Dose-dependent effects of prebiotic treatments were also observed on cecum and fecal bacterial enzymes and on SCFA.
27490566	3	41	theme	incidence	627:635	arg1	biomarkers					581:590	various biomarkers	573:590	various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF)	573:664	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	3	42	theme	present	504:510	arg1	study					512:516	the present study	500:516	the present study	500:516	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	0	43	theme	Inulin-Inhibited	61:76	arg1	Formation					98:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	2	44	from	inulin	454:459	arg1	prevention					471:480	cancer prevention	464:480	cancer prevention	464:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	5	45	theme	significant	929:939	arg1	effect					952:957	the significant preventive effect	925:957	the significant preventive effect of the GOS and inulin combination against the development of CRC	925:1022	Our data reveal the significant preventive effect of the GOS and inulin combination against the development of CRC.
27490566	4	46	theme	GOS	771:773	arg1	doses					822:826	individual doses	811:826	individual doses (GOS: 76-151 mg; inulin 114 mg)	811:858	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	4	46	theme	GOS	771:773	arg1	treatments					748:757	Prebiotic treatments	738:757	Prebiotic treatments of combined GOS and inulin (57 mg each)	738:797	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	2	47	from	linkages	408:415	arg1	prevention					471:480	cancer prevention	464:480	cancer prevention	464:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	48	from	combination	431:441	arg1	prevention					471:480	cancer prevention	464:480	cancer prevention	464:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	3	49	theme	foci	655:658	arg1	CRC					614:616	CRC	614:616	CRC	614:616	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	3	49	theme	foci	655:658	arg1	cancer					606:611	colorectal cancer	595:611	colorectal cancer (CRC)	595:617	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	3	49	theme	foci	655:658	arg1	incidence					627:635	the incidence	623:635	the incidence of aberrant crypt foci (ACF)	623:664	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	4	50	theme	inulin	779:784	arg1	doses					822:826	individual doses	811:826	individual doses (GOS: 76-151 mg; inulin 114 mg)	811:858	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	4	50	theme	inulin	779:784	arg1	treatments					748:757	Prebiotic treatments	738:757	Prebiotic treatments of combined GOS and inulin (57 mg each)	738:797	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	2	51	theme	β-1,3	391:395	arg1	linkages					408:415	β-1,6 and β-1,3 glycosidic linkages	381:415	β-1,6 and β-1,3 glycosidic linkages	381:415	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	7	52	theme	enzymatic	1303:1311	arg1	activities					1313:1322	bacterial enzymatic activities	1293:1322	bacterial enzymatic activities	1293:1322	This combination also rendered better results on short-chain fatty acids (SCFA) and bacterial enzymatic activities.
27490566	2	53	from	GOS	446:448	arg1	prevention					471:480	cancer prevention	464:480	cancer prevention	464:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	2	54	theme	β-1,6	381:385	arg1	linkages					408:415	β-1,6 and β-1,3 glycosidic linkages	381:415	β-1,6 and β-1,3 glycosidic linkages	381:415	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	1	55	theme	monosaccharide	273:286	arg1	units					288:292	monosaccharide units	273:292	monosaccharide units	273:292	The selectivity and beneficial effects of prebiotics are mainly dependent on composition and glycosidic linkage among monosaccharide units.
27490566	9	56	theme	novel	1476:1480	arg1	combination					1482:1492	novel combination	1476:1492	novel combination of GOS and inulin	1476:1510	Thus, this study demonstrated that novel combination of GOS and inulin exhibited stronger preventive activity than their individual treatments alone, and can be a promising strategy for CRC chemoprevention.
27490566	3	57	theme	novel	544:548	arg1	GOS					550:552	novel GOS	544:552	novel GOS	544:552	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	7	58	theme	fatty	1270:1274	arg1	SCFA					1283:1286	SCFA	1283:1286	SCFA	1283:1286	This combination also rendered better results on short-chain fatty acids (SCFA) and bacterial enzymatic activities.
27490566	7	58	theme	fatty	1270:1274	arg1	acids					1276:1280	short-chain fatty acids	1258:1280	short-chain fatty acids (SCFA)	1258:1287	This combination also rendered better results on short-chain fatty acids (SCFA) and bacterial enzymatic activities.
27490566	4	59	dep	inulin	779:784	arg1	mg					790:791	57 mg	787:791	57 mg each	787:796	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	6	60	theme	ACF	1060:1062	arg1	formation					1064:1072	ACF formation	1060:1072	ACF formation (55.8%)	1060:1080	It was observed that inhibition of ACF formation (55.8%) was significantly (p ≤ 0.05) higher using the GOS and inulin combination than GOS (41.4%) and inulin (51.2%) treatments alone.
27490566	6	60	theme	ACF	1060:1062	arg1	%					1079:1079	55.8%	1075:1079	55.8%	1075:1079	It was observed that inhibition of ACF formation (55.8%) was significantly (p ≤ 0.05) higher using the GOS and inulin combination than GOS (41.4%) and inulin (51.2%) treatments alone.
27490566	1	61	theme	prebiotics	197:206	arg1	selectivity					159:169	The selectivity	155:169	The selectivity	155:169	The selectivity and beneficial effects of prebiotics are mainly dependent on composition and glycosidic linkage among monosaccharide units.
27490566	1	61	theme	prebiotics	197:206	arg1	effects					186:192	beneficial effects	175:192	beneficial effects of prebiotics	175:206	The selectivity and beneficial effects of prebiotics are mainly dependent on composition and glycosidic linkage among monosaccharide units.
27490566	1	61	theme	prebiotics	197:206	arg1	dependent					219:227	dependent	219:227	dependent	219:227	The selectivity and beneficial effects of prebiotics are mainly dependent on composition and glycosidic linkage among monosaccharide units.
27490566	7	62	theme	better	1240:1245	arg1	results					1247:1253	better results	1240:1253	better results	1240:1253	This combination also rendered better results on short-chain fatty acids (SCFA) and bacterial enzymatic activities.
27490566	0	63	theme	Wistar	142:147	arg1	Rats					149:152	Wistar Rats	142:152	Wistar Rats	142:152	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	3	64	theme	various	573:579	arg1	biomarkers					581:590	various biomarkers	573:590	various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF)	573:664	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	9	65	theme	preventive	1531:1540	arg1	activity					1542:1549	stronger preventive activity	1522:1549	stronger preventive activity	1522:1549	Thus, this study demonstrated that novel combination of GOS and inulin exhibited stronger preventive activity than their individual treatments alone, and can be a promising strategy for CRC chemoprevention.
27490566	3	66	theme	-induced	715:722	arg1	model					731:735	a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model	669:735	a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model	669:735	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	3	67	theme	colorectal	595:604	arg1	CRC					614:616	CRC	614:616	CRC	614:616	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	3	67	theme	colorectal	595:604	arg1	cancer					606:611	colorectal cancer	595:611	colorectal cancer (CRC)	595:617	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	6	68	theme	≤	1103:1103	arg1	p					1101:1101	p ≤ 0.05	1101:1108	p ≤ 0.05	1101:1108	It was observed that inhibition of ACF formation (55.8%) was significantly (p ≤ 0.05) higher using the GOS and inulin combination than GOS (41.4%) and inulin (51.2%) treatments alone.
27490566	5	69	theme	combination	981:991	arg1	effect					952:957	the significant preventive effect	925:957	the significant preventive effect of the GOS and inulin combination against the development of CRC	925:1022	Our data reveal the significant preventive effect of the GOS and inulin combination against the development of CRC.
27490566	9	70	theme	individual	1562:1571	arg1	treatments					1573:1582	their individual treatments	1556:1582	their individual treatments alone	1556:1588	Thus, this study demonstrated that novel combination of GOS and inulin exhibited stronger preventive activity than their individual treatments alone, and can be a promising strategy for CRC chemoprevention.
27490566	4	71	dep	mg	841:842	arg1	mg					856:857	inulin 114 mg	845:857	76-151 mg; inulin 114 mg	834:857	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	8	72	theme	Dose-dependent	1325:1338	arg1	effects					1340:1346	Dose-dependent effects	1325:1346	Dose-dependent effects of prebiotic treatments	1325:1370	Dose-dependent effects of prebiotic treatments were also observed on cecum and fecal bacterial enzymes and on SCFA.
27490566	3	73	from	role	536:539	arg1	model					731:735	a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model	669:735	a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model	669:735	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	0	74	from	Combination	6:16	arg1	Rats					149:152	Wistar Rats	142:152	Wistar Rats	142:152	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	9	75	theme	promising	1604:1612	arg1	strategy					1614:1621	a promising strategy	1602:1621	a promising strategy for CRC chemoprevention	1602:1645	Thus, this study demonstrated that novel combination of GOS and inulin exhibited stronger preventive activity than their individual treatments alone, and can be a promising strategy for CRC chemoprevention.
27490566	8	76	theme	treatments	1361:1370	arg1	effects					1340:1346	Dose-dependent effects	1325:1346	Dose-dependent effects of prebiotic treatments	1325:1370	Dose-dependent effects of prebiotic treatments were also observed on cecum and fecal bacterial enzymes and on SCFA.
27490566	0	77	theme	Cancer	132:137	arg1	Formation					98:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	0	77	theme	Cancer	132:137	arg1	Combination					6:16	Novel Combination	0:16	Novel Combination of Prebiotics Galacto-Oligosaccharides	0:55	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	0	77	theme	Cancer	132:137	arg1	Biomarkers					112:121	Biomarkers	112:121	Biomarkers of Colon Cancer	112:137	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	8	78	theme	prebiotic	1351:1359	arg1	treatments					1361:1370	prebiotic treatments	1351:1370	prebiotic treatments	1351:1370	Dose-dependent effects of prebiotic treatments were also observed on cecum and fecal bacterial enzymes and on SCFA.
27490566	2	79	theme	glycosidic	397:406	arg1	linkages					408:415	β-1,6 and β-1,3 glycosidic linkages	381:415	β-1,6 and β-1,3 glycosidic linkages	381:415	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	0	80	theme	Prebiotics	21:30	arg1	Galacto-Oligosaccharides					32:55	Prebiotics Galacto-Oligosaccharides	21:55	Prebiotics Galacto-Oligosaccharides	21:55	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	8	81	theme	bacterial	1410:1418	arg1	enzymes					1420:1426	fecal bacterial enzymes	1404:1426	fecal bacterial enzymes	1404:1426	Dose-dependent effects of prebiotic treatments were also observed on cecum and fecal bacterial enzymes and on SCFA.
27490566	2	82	theme	novel	425:429	arg1	combination					431:441	the novel combination	421:441	the novel combination of GOS and inulin in cancer prevention	421:480	This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention.
27490566	0	83	theme	Aberrant	78:85	arg1	Formation					98:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Inulin-Inhibited Aberrant Crypt Foci Formation	61:106	Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats.
27490566	3	84	theme	study	512:516	arg1	objective					487:495	The objective	483:495	The objective of the present study	483:516	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	9	85	theme	stronger	1522:1529	arg1	activity					1542:1549	stronger preventive activity	1522:1549	stronger preventive activity	1522:1549	Thus, this study demonstrated that novel combination of GOS and inulin exhibited stronger preventive activity than their individual treatments alone, and can be a promising strategy for CRC chemoprevention.
27490566	3	86	theme	crypt	649:653	arg1	foci					655:658	aberrant crypt foci	640:658	aberrant crypt foci (ACF)	640:664	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	3	86	theme	crypt	649:653	arg1	ACF					661:663	ACF	661:663	ACF	661:663	The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model.
27490566	4	87	theme	combined	762:769	arg1	GOS					771:773	combined GOS	762:773	combined GOS	762:773	Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76-151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks.
27490566	1	88	theme	glycosidic	248:257	arg1	linkage					259:265	glycosidic linkage	248:265	glycosidic linkage	248:265	The selectivity and beneficial effects of prebiotics are mainly dependent on composition and glycosidic linkage among monosaccharide units.
27490566	5	89	dep	GOS	966:968	arg1	the					962:964	the	962:964	the	962:964	Our data reveal the significant preventive effect of the GOS and inulin combination against the development of CRC.
27490566	9	90	theme	CRC	1627:1629	arg1	chemoprevention					1631:1645	CRC chemoprevention	1627:1645	CRC chemoprevention	1627:1645	Thus, this study demonstrated that novel combination of GOS and inulin exhibited stronger preventive activity than their individual treatments alone, and can be a promising strategy for CRC chemoprevention.
27474582	3	0	theme	composite	448:456	arg1	electrode					467:475	The composite modified electrode	444:475	The composite modified electrode	444:475	The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine.
27474582	2	1	theme	micromolar	425:434	arg1	levels					436:441	its sub micromolar levels	417:441	its sub micromolar levels	417:441	In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels.
27474582	5	2	theme	composite	822:830	arg1	electrode					841:849	the fabricated GR-CS composite modified electrode	801:849	the fabricated GR-CS composite modified electrode	801:849	Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06μM.
27474582	1	3	theme	levels	150:155	arg1	indicators					223:232	essential indicators	213:232	essential indicators in medical diagnostics	213:255	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	1	3	theme	levels	150:155	arg1	detection					123:131	The accurate detection	110:131	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine	110:207	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	1	4	theme	medical	237:243	arg1	diagnostics					245:255	medical diagnostics	237:255	medical diagnostics	237:255	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	2	5	from	detection	398:406	arg1	levels					436:441	its sub micromolar levels	417:441	its sub micromolar levels	417:441	In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels.
27474582	4	6	theme	DA	706:707	arg1	detection					693:701	the detection	689:701	the detection of DA	689:707	The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely.
27474582	0	7	theme	biological	90:99	arg1	samples					101:107	biological samples	90:107	biological samples	90:107	Preparation of chitosan grafted graphite composite for sensitive detection of dopamine in biological samples.
27474582	5	8	theme	linear	887:892	arg1	response					894:901	the linear response	883:901	the linear response ranging from 0.03 to 20.06μM	883:930	Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06μM.
27474582	5	9	theme	DPV	861:863	arg1	response					865:872	the DPV response	857:872	the DPV response of DA in the linear response ranging from 0.03 to 20.06μM	857:930	Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06μM.
27474582	4	10	theme	current	640:646	arg1	response					648:655	an enhanced oxidation peak current response	613:655	an enhanced oxidation peak current response	613:655	The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely.
27474582	4	11	theme	low	661:663	arg1	potential					675:683	low oxidation potential	661:683	low oxidation potential for the detection of DA	661:707	The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely.
27474582	4	12	theme	peak	635:638	arg1	response					648:655	an enhanced oxidation peak current response	613:655	an enhanced oxidation peak current response	613:655	The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely.
27474582	2	13	theme	sensitive	368:376	arg1	detection					398:406	the sensitive and lower potential detection	364:406	the sensitive and lower potential detection of DA in its sub micromolar levels	364:441	In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels.
27474582	2	14	theme	composite	350:358	arg1	CS					313:314	CS	313:314	CS	313:314	In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels.
27474582	2	14	theme	composite	350:358	arg1	chitosan					303:310	chitosan	303:310	chitosan (CS) biopolymer grafted graphite (GR) composite	303:358	In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels.
27474582	5	15	theme	modified	832:839	arg1	electrode					841:849	the fabricated GR-CS composite modified electrode	801:849	the fabricated GR-CS composite modified electrode	801:849	Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06μM.
27474582	6	16	theme	detection	937:945	arg1	0.0045μM					1001:1008	0.0045μM	1001:1008	0.0045μM	1001:1008	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	16	theme	detection	937:945	arg1	cm					1027:1028	6.06μA μM(-1)cm	1014:1028	6.06μA μM(-1)cm(-2)	1014:1032	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	16	theme	detection	937:945	arg1	sensitivity					957:967	sensitivity	957:967	sensitivity	957:967	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	16	theme	detection	937:945	arg1	limit					947:951	The detection limit	933:951	The detection limit	933:951	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	1	17	theme	biological	160:169	arg1	samples					171:177	biological samples	160:177	biological samples such as human serum and urine	160:207	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	1	17	theme	biological	160:169	arg1	urine					203:207	urine	203:207	urine	203:207	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	1	17	theme	biological	160:169	arg1	serum					193:197	human serum	187:197	human serum	187:197	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	0	18	theme	dopamine	78:85	arg1	detection					65:73	sensitive detection	55:73	sensitive detection of dopamine in biological samples	55:107	Preparation of chitosan grafted graphite composite for sensitive detection of dopamine in biological samples.
27474582	3	19	theme	DA	512:513	arg1	detection					499:507	the detection	495:507	the detection of DA in biological samples such as human serum and urine	495:565	The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine.
27474582	5	20	theme	optimum	781:787	arg1	conditions					789:798	optimum conditions	781:798	optimum conditions	781:798	Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06μM.
27474582	5	21	theme	GR-CS	816:820	arg1	electrode					841:849	the fabricated GR-CS composite modified electrode	801:849	the fabricated GR-CS composite modified electrode	801:849	Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06μM.
27474582	6	22	theme	sensor	976:981	arg1	0.0045μM					1001:1008	0.0045μM	1001:1008	0.0045μM	1001:1008	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	22	theme	sensor	976:981	arg1	cm					1027:1028	6.06μA μM(-1)cm	1014:1028	6.06μA μM(-1)cm(-2)	1014:1032	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	22	theme	sensor	976:981	arg1	sensitivity					957:967	sensitivity	957:967	sensitivity	957:967	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	22	theme	sensor	976:981	arg1	limit					947:951	The detection limit	933:951	The detection limit	933:951	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	2	23	theme	potential	388:396	arg1	detection					398:406	the sensitive and lower potential detection	364:406	the sensitive and lower potential detection of DA in its sub micromolar levels	364:441	In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels.
27474582	1	24	from	detection	123:131	arg1	samples					171:177	biological samples	160:177	biological samples such as human serum and urine	160:207	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	1	24	from	detection	123:131	arg1	urine					203:207	urine	203:207	urine	203:207	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	1	24	from	detection	123:131	arg1	serum					193:197	human serum	187:197	human serum	187:197	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	5	25	theme	DA	877:878	arg1	response					865:872	the DPV response	857:872	the DPV response of DA in the linear response ranging from 0.03 to 20.06μM	857:930	Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06μM.
27474582	0	26	theme	chitosan	15:22	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan	0:22	Preparation of chitosan grafted graphite composite for sensitive detection of dopamine in biological samples.
27474582	3	27	theme	modified	458:465	arg1	electrode					467:475	The composite modified electrode	444:475	The composite modified electrode	444:475	The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine.
27474582	1	28	theme	human	187:191	arg1	serum					193:197	human serum	187:197	human serum	187:197	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	2	29	theme	sub	421:423	arg1	levels					436:441	its sub micromolar levels	417:441	its sub micromolar levels	417:441	In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels.
27474582	4	30	theme	composite	578:586	arg1	electrode					597:605	The GR-CS composite modified electrode	568:605	The GR-CS composite modified electrode	568:605	The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely.
27474582	0	31	theme	graphite	32:39	arg1	composite					41:49	graphite composite	32:49	graphite composite	32:49	Preparation of chitosan grafted graphite composite for sensitive detection of dopamine in biological samples.
27474582	4	32	theme	GR-CS	572:576	arg1	electrode					597:605	The GR-CS composite modified electrode	568:605	The GR-CS composite modified electrode	568:605	The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely.
27474582	0	33	theme	sensitive	55:63	arg1	detection					65:73	sensitive detection	55:73	sensitive detection of dopamine in biological samples	55:107	Preparation of chitosan grafted graphite composite for sensitive detection of dopamine in biological samples.
27474582	5	34	theme	fabricated	805:814	arg1	electrode					841:849	the fabricated GR-CS composite modified electrode	801:849	the fabricated GR-CS composite modified electrode	801:849	Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06μM.
27474582	0	35	from	detection	65:73	arg1	samples					101:107	biological samples	90:107	biological samples	90:107	Preparation of chitosan grafted graphite composite for sensitive detection of dopamine in biological samples.
27474582	3	36	theme	biological	518:527	arg1	serum					551:555	human serum	545:555	human serum	545:555	The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine.
27474582	3	36	theme	biological	518:527	arg1	samples					529:535	biological samples	518:535	biological samples such as human serum and urine	518:565	The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine.
27474582	3	36	theme	biological	518:527	arg1	urine					561:565	urine	561:565	urine	561:565	The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine.
27474582	6	37	theme	6.06μA	1014:1019	arg1	limit					947:951	The detection limit	933:951	The detection limit	933:951	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	37	theme	6.06μA	1014:1019	arg1	-2					1030:1031	-2	1030:1031	-2	1030:1031	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	37	theme	6.06μA	1014:1019	arg1	sensitivity					957:967	sensitivity	957:967	sensitivity	957:967	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	37	theme	6.06μA	1014:1019	arg1	cm					1027:1028	6.06μA μM(-1)cm	1014:1028	6.06μA μM(-1)cm(-2)	1014:1032	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	1	38	theme	accurate	114:121	arg1	indicators					223:232	essential indicators	213:232	essential indicators in medical diagnostics	213:255	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	1	38	theme	accurate	114:121	arg1	detection					123:131	The accurate detection	110:131	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine	110:207	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	2	39	theme	DA	411:412	arg1	detection					398:406	the sensitive and lower potential detection	364:406	the sensitive and lower potential detection of DA in its sub micromolar levels	364:441	In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels.
27474582	1	40	from	indicators	223:232	arg1	diagnostics					245:255	medical diagnostics	237:255	medical diagnostics	237:255	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	3	41	from	detection	499:507	arg1	serum					551:555	human serum	545:555	human serum	545:555	The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine.
27474582	3	41	from	detection	499:507	arg1	samples					529:535	biological samples	518:535	biological samples such as human serum and urine	518:565	The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine.
27474582	3	41	from	detection	499:507	arg1	urine					561:565	urine	561:565	urine	561:565	The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine.
27474582	2	42	theme	chitosan	303:310	arg1	preparation					288:298	the preparation	284:298	the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels	284:441	In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels.
27474582	1	43	theme	dopamine	136:143	arg1	levels					150:155	dopamine (DA) levels	136:155	dopamine (DA) levels	136:155	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	5	44	from	response	865:872	arg1	response					894:901	the linear response	883:901	the linear response ranging from 0.03 to 20.06μM	883:930	Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06μM.
27474582	4	45	theme	oxidation	665:673	arg1	potential					675:683	low oxidation potential	661:683	low oxidation potential for the detection of DA	661:707	The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely.
27474582	3	46	used	used	486:489	arg2	electrode					467:475	The composite modified electrode	444:475	The composite modified electrode	444:475	The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine.
27474582	3	47	theme	human	545:549	arg1	serum					551:555	human serum	545:555	human serum	545:555	The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine.
27474582	4	48	theme	oxidation	625:633	arg1	response					648:655	an enhanced oxidation peak current response	613:655	an enhanced oxidation peak current response	613:655	The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely.
27474582	4	49	theme	modified	588:595	arg1	electrode					597:605	The GR-CS composite modified electrode	568:605	The GR-CS composite modified electrode	568:605	The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely.
27474582	1	50	theme	essential	213:221	arg1	indicators					223:232	essential indicators	213:232	essential indicators in medical diagnostics	213:255	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	1	50	theme	essential	213:221	arg1	detection					123:131	The accurate detection	110:131	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine	110:207	The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics.
27474582	6	51	theme	μM	1021:1022	arg1	limit					947:951	The detection limit	933:951	The detection limit	933:951	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	51	theme	μM	1021:1022	arg1	-2					1030:1031	-2	1030:1031	-2	1030:1031	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	51	theme	μM	1021:1022	arg1	sensitivity					957:967	sensitivity	957:967	sensitivity	957:967	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	6	51	theme	μM	1021:1022	arg1	cm					1027:1028	6.06μA μM(-1)cm	1014:1028	6.06μA μM(-1)cm(-2)	1014:1032	The detection limit and sensitivity of the sensor were estimated as 0.0045μM and 6.06μA μM(-1)cm(-2), respectively.
27474582	4	52	theme	enhanced	616:623	arg1	response					648:655	an enhanced oxidation peak current response	613:655	an enhanced oxidation peak current response	613:655	The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely.
25410797	6	0	theme	bare	1077:1080	arg1	QDs					1082:1084	bare QDs	1077:1084	bare QDs	1077:1084	Further, viability of NIH3T3 mouse fibroblast cells in vitro increased in presence of QDs-embedded CNPs as compared to bare QDs.
25410797	10	1	theme	QDs-embedded	1457:1468	arg1	CNPs					1470:1473	QDs-embedded CNPs	1457:1473	QDs-embedded CNPs	1457:1473	Moreover, QDs-embedded CNPs show enhanced internalization in NIH3T3 cells as compared to bare QDs.
25410797	3	2	theme	telluride	509:517	arg1	QDs					538:540	CdTe QDs	533:540	CdTe QDs	533:540	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	3	2	theme	telluride	509:517	arg1	dots					527:530	green fluorescent cadmium telluride quantum dots	483:530	green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs	483:563	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	7	3	from	48 h	1188:1191	arg1	viability					1168:1176	viability	1168:1176	viability at 24 and 48 h, respectively	1168:1205	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	7	3	from	48 h	1188:1191	arg1	increase					1156:1163	34 and 39% increase	1145:1163	34 and 39% increase in viability at 24 and 48 h, respectively	1145:1205	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	10	4	theme	NIH3T3	1508:1513	arg1	cells					1515:1519	NIH3T3 cells	1508:1519	NIH3T3 cells	1508:1519	Moreover, QDs-embedded CNPs show enhanced internalization in NIH3T3 cells as compared to bare QDs.
25410797	1	5	theme	high	259:262	arg1	cytotoxicity					264:275	high cytotoxicity	259:275	high cytotoxicity	259:275	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	2	6	theme	good	377:380	arg1	biocompatibility					382:397	good biocompatibility	377:397	good biocompatibility	377:397	Nanoparticles synthesized from chitosan, a natural biopolymer of β 1-4 linked glucosamine, display good biocompatibility and cellular uptake.
25410797	4	7	theme	electron	642:649	arg1	microscopy					651:660	High-resolution transmission electron microscopy	613:660	High-resolution transmission electron microscopy	613:660	High-resolution transmission electron microscopy showed the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition.
25410797	3	8	theme	CdTe	533:536	arg1	QDs					538:540	CdTe QDs	533:540	CdTe QDs	533:540	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	3	8	theme	CdTe	533:536	arg1	dots					527:530	green fluorescent cadmium telluride quantum dots	483:530	green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs	483:563	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	11	9	with	CNPs	1652:1655	arg1	profile					1683:1689	a favorable toxicity profile	1662:1689	a favorable toxicity profile	1662:1689	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	11	9	with	CNPs	1652:1655	arg1	uptake					1711:1716	better cellular uptake	1695:1716	better cellular uptake	1695:1716	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	9	10	theme	hydrolytic	1357:1366	arg1	enzymes					1368:1374	hydrolytic enzymes	1357:1374	hydrolytic enzymes such as lysozyme and chitinase	1357:1405	Furthermore, hydrolytic enzymes such as lysozyme and chitinase did not degrade chitosan nanoparticles.
25410797	9	10	theme	hydrolytic	1357:1366	arg1	lysozyme					1384:1391	lysozyme	1384:1391	lysozyme	1384:1391	Furthermore, hydrolytic enzymes such as lysozyme and chitinase did not degrade chitosan nanoparticles.
25410797	9	10	theme	hydrolytic	1357:1366	arg1	chitinase					1397:1405	chitinase	1397:1405	chitinase	1397:1405	Furthermore, hydrolytic enzymes such as lysozyme and chitinase did not degrade chitosan nanoparticles.
25410797	3	11	theme	biocompatible	546:558	arg1	CNPs					560:563	biocompatible CNPs	546:563	biocompatible CNPs	546:563	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	1	12	theme	attractive	200:209	arg1	dots					167:170	Fluorescent cadmium telluride quantum dots	129:170	Fluorescent cadmium telluride quantum dots (CdTe QDs)	129:181	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	1	12	theme	attractive	200:209	arg1	option					211:216	an optically attractive option	187:216	an optically attractive option for bioimaging	187:231	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	4	13	theme	High-resolution	613:627	arg1	microscopy					651:660	High-resolution transmission electron microscopy	613:660	High-resolution transmission electron microscopy	613:660	High-resolution transmission electron microscopy showed the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition.
25410797	7	14	from	increase	1156:1163	arg1	viability					1168:1176	viability	1168:1176	viability at 24 and 48 h, respectively	1168:1205	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	7	14	from	increase	1156:1163	arg1	48 h					1188:1191	48 h	1188:1191	48 h	1188:1191	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	7	14	from	increase	1156:1163	arg1	24					1181:1182	24	1181:1182	24	1181:1182	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	4	15	theme	CdTe	706:709	arg1	QDs					711:713	CdTe QDs	706:713	CdTe QDs embedded in CNPs	706:730	High-resolution transmission electron microscopy showed the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition.
25410797	1	16	theme	Fluorescent	129:139	arg1	dots					167:170	Fluorescent cadmium telluride quantum dots	129:170	Fluorescent cadmium telluride quantum dots (CdTe QDs)	129:181	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	1	16	theme	Fluorescent	129:139	arg1	QDs					178:180	CdTe QDs	173:180	CdTe QDs	173:180	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	1	16	theme	Fluorescent	129:139	arg1	option					211:216	an optically attractive option	187:216	an optically attractive option for bioimaging	187:231	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	5	17	dep	months	950:955	arg1	up					938:939	up	938:939	up	938:939	Electrostatic interactions between thiol-capped QDs (4 nm, -57 mV) and CNPs (~300 nm, +38 mV) generated CdTe QDs-embedded CNPs that were stable up to three months.
25410797	11	18	theme	toxicity	1674:1681	arg1	profile					1683:1689	a favorable toxicity profile	1662:1689	a favorable toxicity profile	1662:1689	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	9	19	theme	chitosan	1423:1430	arg1	nanoparticles					1432:1444	chitosan nanoparticles	1423:1444	chitosan nanoparticles	1423:1444	Furthermore, hydrolytic enzymes such as lysozyme and chitinase did not degrade chitosan nanoparticles.
25410797	4	20	theme	embedded	715:722	arg1	QDs					711:713	CdTe QDs	706:713	CdTe QDs embedded in CNPs	706:730	High-resolution transmission electron microscopy showed the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition.
25410797	7	21	from	24	1181:1182	arg1	viability					1168:1176	viability	1168:1176	viability at 24 and 48 h, respectively	1168:1205	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	7	21	from	24	1181:1182	arg1	increase					1156:1163	34 and 39% increase	1145:1163	34 and 39% increase in viability at 24 and 48 h, respectively	1145:1205	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	4	22	theme	crystal	673:679	arg1	lattice					681:687	the crystal lattice	669:687	the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition	669:791	High-resolution transmission electron microscopy showed the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition.
25410797	6	23	theme	fibroblast	993:1002	arg1	cells					1004:1008	NIH3T3 mouse fibroblast cells	980:1008	NIH3T3 mouse fibroblast cells	980:1008	Further, viability of NIH3T3 mouse fibroblast cells in vitro increased in presence of QDs-embedded CNPs as compared to bare QDs.
25410797	11	24	theme	cellular	1702:1709	arg1	uptake					1711:1716	better cellular uptake	1695:1716	better cellular uptake	1695:1716	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	0	25	theme	stable	78:83	arg1	preparation					100:110	a stable, biocompatible preparation	76:110	chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging	52:126	Fluorescent cadmium telluride quantum dots embedded chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging.
25410797	5	26	theme	CdTe	898:901	arg1	CNPs					916:919	CdTe QDs-embedded CNPs	898:919	CdTe QDs-embedded CNPs that were stable up to three months	898:955	Electrostatic interactions between thiol-capped QDs (4 nm, -57 mV) and CNPs (~300 nm, +38 mV) generated CdTe QDs-embedded CNPs that were stable up to three months.
25410797	6	27	theme	NIH3T3	980:985	arg1	cells					1004:1008	NIH3T3 mouse fibroblast cells	980:1008	NIH3T3 mouse fibroblast cells	980:1008	Further, viability of NIH3T3 mouse fibroblast cells in vitro increased in presence of QDs-embedded CNPs as compared to bare QDs.
25410797	1	28	theme	telluride	149:157	arg1	dots					167:170	Fluorescent cadmium telluride quantum dots	129:170	Fluorescent cadmium telluride quantum dots (CdTe QDs)	129:181	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	1	28	theme	telluride	149:157	arg1	QDs					178:180	CdTe QDs	173:180	CdTe QDs	173:180	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	1	28	theme	telluride	149:157	arg1	option					211:216	an optically attractive option	187:216	an optically attractive option for bioimaging	187:231	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	2	29	theme	natural	321:327	arg1	Nanoparticles					278:290	Nanoparticles	278:290	Nanoparticles synthesized from chitosan	278:316	Nanoparticles synthesized from chitosan, a natural biopolymer of β 1-4 linked glucosamine, display good biocompatibility and cellular uptake.
25410797	2	29	theme	natural	321:327	arg1	biopolymer					329:338	a natural biopolymer	319:338	a natural biopolymer of β 1-4 linked glucosamine	319:366	Nanoparticles synthesized from chitosan, a natural biopolymer of β 1-4 linked glucosamine, display good biocompatibility and cellular uptake.
25410797	3	30	theme	facile	422:427	arg1	strategy					446:453	A facile, green synthetic strategy	420:453	A facile, green synthetic strategy	420:453	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	0	31	theme	Fluorescent	0:10	arg1	dots					38:41	Fluorescent cadmium telluride quantum dots	0:41	Fluorescent cadmium telluride quantum dots	0:41	Fluorescent cadmium telluride quantum dots embedded chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging.
25410797	4	32	from	CNPs	727:730	arg1	embedded					715:722	embedded	715:722	embedded	715:722	High-resolution transmission electron microscopy showed the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition.
25410797	0	33	dep	stable	78:83	arg1	biocompatible					86:98	biocompatible	86:98	biocompatible	86:98	Fluorescent cadmium telluride quantum dots embedded chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging.
25410797	6	34	theme	QDs-embedded	1044:1055	arg1	CNPs					1057:1060	QDs-embedded CNPs	1044:1060	QDs-embedded CNPs	1044:1060	Further, viability of NIH3T3 mouse fibroblast cells in vitro increased in presence of QDs-embedded CNPs as compared to bare QDs.
25410797	0	35	dep	nanoparticles	61:73	arg1	preparation					100:110	a stable, biocompatible preparation	76:110	chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging	52:126	Fluorescent cadmium telluride quantum dots embedded chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging.
25410797	0	36	theme	telluride	20:28	arg1	dots					38:41	Fluorescent cadmium telluride quantum dots	0:41	Fluorescent cadmium telluride quantum dots	0:41	Fluorescent cadmium telluride quantum dots embedded chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging.
25410797	5	37	theme	Electrostatic	794:806	arg1	interactions					808:819	Electrostatic interactions	794:819	Electrostatic interactions between thiol-capped QDs (4 nm, -57 mV) and CNPs (~300 nm, +38 mV)	794:886	Electrostatic interactions between thiol-capped QDs (4 nm, -57 mV) and CNPs (~300 nm, +38 mV) generated CdTe QDs-embedded CNPs that were stable up to three months.
25410797	5	38	dep	CNPs	865:868	arg1	+38 mV					880:885	+38 mV	880:885	+38 mV	880:885	Electrostatic interactions between thiol-capped QDs (4 nm, -57 mV) and CNPs (~300 nm, +38 mV) generated CdTe QDs-embedded CNPs that were stable up to three months.
25410797	5	38	dep	CNPs	865:868	arg1	~300 nm					871:877	~300 nm	871:877	~300 nm	871:877	Electrostatic interactions between thiol-capped QDs (4 nm, -57 mV) and CNPs (~300 nm, +38 mV) generated CdTe QDs-embedded CNPs that were stable up to three months.
25410797	2	39	theme	β	343:343	arg1	Nanoparticles					278:290	Nanoparticles	278:290	Nanoparticles synthesized from chitosan	278:316	Nanoparticles synthesized from chitosan, a natural biopolymer of β 1-4 linked glucosamine, display good biocompatibility and cellular uptake.
25410797	2	39	theme	β	343:343	arg1	biopolymer					329:338	a natural biopolymer	319:338	a natural biopolymer of β 1-4 linked glucosamine	319:366	Nanoparticles synthesized from chitosan, a natural biopolymer of β 1-4 linked glucosamine, display good biocompatibility and cellular uptake.
25410797	0	40	theme	chitosan	52:59	arg1	nanoparticles					61:73	chitosan nanoparticles	52:73	chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging	52:126	Fluorescent cadmium telluride quantum dots embedded chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging.
25410797	7	41	dep	%	1154:1154	arg1	39					1152:1153	39	1152:1153	39	1152:1153	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	7	41	dep	%	1154:1154	arg1	34					1145:1146	34	1145:1146	34	1145:1146	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	11	42	theme	cellular	1774:1781	arg1	components					1783:1792	cellular components	1774:1792	cellular components	1774:1792	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	10	43	theme	enhanced	1480:1487	arg1	internalization					1489:1503	enhanced internalization	1480:1503	enhanced internalization in NIH3T3 cells	1480:1519	Moreover, QDs-embedded CNPs show enhanced internalization in NIH3T3 cells as compared to bare QDs.
25410797	11	44	theme	QDs-embedded	1639:1650	arg1	CNPs					1652:1655	stable, luminescent, biocompatible CdTe QDs-embedded CNPs	1599:1655	stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components	1599:1792	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	3	45	theme	quantum	519:525	arg1	QDs					538:540	CdTe QDs	533:540	CdTe QDs	533:540	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	3	45	theme	quantum	519:525	arg1	dots					527:530	green fluorescent cadmium telluride quantum dots	483:530	green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs	483:563	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	11	46	theme	synthesis	1573:1581	arg1	ease					1565:1568	ease	1565:1568	ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components	1565:1792	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	7	47	theme	highest	1094:1100	arg1	10 μg/ml					1117:1124	10 μg/ml	1117:1124	10 μg/ml	1117:1124	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	7	47	theme	highest	1094:1100	arg1	concentration					1102:1114	the highest concentration	1090:1114	the highest concentration (10 μg/ml)	1090:1125	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	3	48	theme	cadmium	501:507	arg1	QDs					538:540	CdTe QDs	533:540	CdTe QDs	533:540	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	3	48	theme	cadmium	501:507	arg1	dots					527:530	green fluorescent cadmium telluride quantum dots	483:530	green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs	483:563	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	5	49	dep	QDs	842:844	arg1	-57 mV					853:858	-57 mV	853:858	-57 mV	853:858	Electrostatic interactions between thiol-capped QDs (4 nm, -57 mV) and CNPs (~300 nm, +38 mV) generated CdTe QDs-embedded CNPs that were stable up to three months.
25410797	5	49	dep	QDs	842:844	arg1	4 nm					847:850	4 nm	847:850	4 nm	847:850	Electrostatic interactions between thiol-capped QDs (4 nm, -57 mV) and CNPs (~300 nm, +38 mV) generated CdTe QDs-embedded CNPs that were stable up to three months.
25410797	11	50	theme	stable	1599:1604	arg1	CNPs					1652:1655	stable, luminescent, biocompatible CdTe QDs-embedded CNPs	1599:1655	stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components	1599:1792	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	3	51	from	dots	527:530	arg1	CNPs					560:563	biocompatible CNPs	546:563	biocompatible CNPs	546:563	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	8	52	dep	48 h	1303:1306	arg1	up					1297:1298	up	1297:1298	up	1297:1298	This shows that chitosan nanoparticles do not release the QDs up to 48 h and do not cause extended toxicity.
25410797	4	53	theme	transmission	629:640	arg1	microscopy					651:660	High-resolution transmission electron microscopy	613:660	High-resolution transmission electron microscopy	613:660	High-resolution transmission electron microscopy showed the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition.
25410797	10	54	theme	bare	1536:1539	arg1	QDs					1541:1543	bare QDs	1536:1543	bare QDs	1536:1543	Moreover, QDs-embedded CNPs show enhanced internalization in NIH3T3 cells as compared to bare QDs.
25410797	11	55	theme	handling	1587:1594	arg1	ease					1565:1568	ease	1565:1568	ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components	1565:1792	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	11	56	theme	bioimaging	1737:1746	arg1	detection					1761:1769	bioimaging and targeted detection	1737:1769	bioimaging and targeted detection of cellular components	1737:1792	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	1	57	theme	CdTe	173:176	arg1	dots					167:170	Fluorescent cadmium telluride quantum dots	129:170	Fluorescent cadmium telluride quantum dots (CdTe QDs)	129:181	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	1	57	theme	CdTe	173:176	arg1	QDs					178:180	CdTe QDs	173:180	CdTe QDs	173:180	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	3	58	theme	safer	577:581	arg1	preparation					583:593	a safer preparation	575:593	a safer preparation than 'as is' QDs	575:610	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	5	59	theme	QDs-embedded	903:914	arg1	CNPs					916:919	CdTe QDs-embedded CNPs	898:919	CdTe QDs-embedded CNPs that were stable up to three months	898:955	Electrostatic interactions between thiol-capped QDs (4 nm, -57 mV) and CNPs (~300 nm, +38 mV) generated CdTe QDs-embedded CNPs that were stable up to three months.
25410797	11	60	theme	targeted	1752:1759	arg1	detection					1761:1769	bioimaging and targeted detection	1737:1769	bioimaging and targeted detection of cellular components	1737:1792	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	11	61	with	uptake	1711:1716	arg1	potential					1723:1731	potential	1723:1731	potential for bioimaging and targeted detection of cellular components	1723:1792	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	1	62	theme	cadmium	141:147	arg1	dots					167:170	Fluorescent cadmium telluride quantum dots	129:170	Fluorescent cadmium telluride quantum dots (CdTe QDs)	129:181	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	1	62	theme	cadmium	141:147	arg1	QDs					178:180	CdTe QDs	173:180	CdTe QDs	173:180	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	1	62	theme	cadmium	141:147	arg1	option					211:216	an optically attractive option	187:216	an optically attractive option for bioimaging	187:231	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	1	63	theme	quantum	159:165	arg1	dots					167:170	Fluorescent cadmium telluride quantum dots	129:170	Fluorescent cadmium telluride quantum dots (CdTe QDs)	129:181	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	1	63	theme	quantum	159:165	arg1	QDs					178:180	CdTe QDs	173:180	CdTe QDs	173:180	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	1	63	theme	quantum	159:165	arg1	option					211:216	an optically attractive option	187:216	an optically attractive option for bioimaging	187:231	Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity.
25410797	6	64	theme	cells	1004:1008	arg1	viability					967:975	viability	967:975	viability of NIH3T3 mouse fibroblast cells in vitro	967:1017	Further, viability of NIH3T3 mouse fibroblast cells in vitro increased in presence of QDs-embedded CNPs as compared to bare QDs.
25410797	11	65	theme	favorable	1664:1672	arg1	profile					1683:1689	a favorable toxicity profile	1662:1689	a favorable toxicity profile	1662:1689	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	11	66	with	profile	1683:1689	arg1	potential					1723:1731	potential	1723:1731	potential for bioimaging and targeted detection of cellular components	1723:1792	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	6	67	theme	mouse	987:991	arg1	cells					1004:1008	NIH3T3 mouse fibroblast cells	980:1008	NIH3T3 mouse fibroblast cells	980:1008	Further, viability of NIH3T3 mouse fibroblast cells in vitro increased in presence of QDs-embedded CNPs as compared to bare QDs.
25410797	8	68	theme	chitosan	1251:1258	arg1	nanoparticles					1260:1272	chitosan nanoparticles	1251:1272	chitosan nanoparticles	1251:1272	This shows that chitosan nanoparticles do not release the QDs up to 48 h and do not cause extended toxicity.
25410797	11	69	theme	better	1695:1700	arg1	uptake					1711:1716	better cellular uptake	1695:1716	better cellular uptake	1695:1716	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	4	70	theme	polymeric	771:779	arg1	composition					781:791	their polymeric composition	765:791	their polymeric composition	765:791	High-resolution transmission electron microscopy showed the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition.
25410797	3	71	theme	synthetic	436:444	arg1	strategy					446:453	A facile, green synthetic strategy	420:453	A facile, green synthetic strategy	420:453	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	0	72	theme	cadmium	12:18	arg1	dots					38:41	Fluorescent cadmium telluride quantum dots	0:41	Fluorescent cadmium telluride quantum dots	0:41	Fluorescent cadmium telluride quantum dots embedded chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging.
25410797	6	73	theme	CNPs	1057:1060	arg1	presence					1032:1039	presence	1032:1039	presence of QDs-embedded CNPs	1032:1060	Further, viability of NIH3T3 mouse fibroblast cells in vitro increased in presence of QDs-embedded CNPs as compared to bare QDs.
25410797	0	74	theme	quantum	30:36	arg1	dots					38:41	Fluorescent cadmium telluride quantum dots	0:41	Fluorescent cadmium telluride quantum dots	0:41	Fluorescent cadmium telluride quantum dots embedded chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging.
25410797	3	75	dep	facile	422:427	arg1	green					430:434	green	430:434	green	430:434	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	2	76	theme	cellular	403:410	arg1	uptake					412:417	cellular uptake	403:417	cellular uptake	403:417	Nanoparticles synthesized from chitosan, a natural biopolymer of β 1-4 linked glucosamine, display good biocompatibility and cellular uptake.
25410797	11	77	theme	CNPs	1652:1655	arg1	handling					1587:1594	handling	1587:1594	handling	1587:1594	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	11	77	theme	CNPs	1652:1655	arg1	synthesis					1573:1581	synthesis	1573:1581	synthesis	1573:1581	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	3	78	theme	fluorescent	489:499	arg1	QDs					538:540	CdTe QDs	533:540	CdTe QDs	533:540	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	3	78	theme	fluorescent	489:499	arg1	dots					527:530	green fluorescent cadmium telluride quantum dots	483:530	green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs	483:563	A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs.
25410797	4	79	from	embedded	715:722	arg1	CNPs					727:730	CNPs	727:730	CNPs	727:730	High-resolution transmission electron microscopy showed the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition.
25410797	11	80	dep	stable	1599:1604	arg1	biocompatible					1620:1632	biocompatible	1620:1632	biocompatible	1620:1632	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	11	80	dep	stable	1599:1604	arg1	luminescent					1607:1617	luminescent	1607:1617	luminescent	1607:1617	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	10	81	from	internalization	1489:1503	arg1	cells					1515:1519	NIH3T3 cells	1508:1519	NIH3T3 cells	1508:1519	Moreover, QDs-embedded CNPs show enhanced internalization in NIH3T3 cells as compared to bare QDs.
25410797	7	82	dep	increase	1156:1163	arg1	%					1154:1154	%	1154:1154	%	1154:1154	At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter.
25410797	11	83	theme	CdTe	1634:1637	arg1	CNPs					1652:1655	stable, luminescent, biocompatible CdTe QDs-embedded CNPs	1599:1655	stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components	1599:1792	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	8	84	theme	extended	1325:1332	arg1	toxicity					1334:1341	extended toxicity	1325:1341	extended toxicity	1325:1341	This shows that chitosan nanoparticles do not release the QDs up to 48 h and do not cause extended toxicity.
25410797	11	85	theme	components	1783:1792	arg1	detection					1761:1769	bioimaging and targeted detection	1737:1769	bioimaging and targeted detection of cellular components	1737:1792	This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
25410797	5	86	theme	thiol-capped	829:840	arg1	QDs					842:844	thiol-capped QDs	829:844	thiol-capped QDs (4 nm, -57 mV)	829:859	Electrostatic interactions between thiol-capped QDs (4 nm, -57 mV) and CNPs (~300 nm, +38 mV) generated CdTe QDs-embedded CNPs that were stable up to three months.
24030688	11	0	theme	rRNA	1197:1200	arg1	genes					1223:1227	the 23S rRNA, gyrB, rpoD and secA genes	1189:1227	the 23S rRNA, gyrB, rpoD and secA genes	1189:1227	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T).
24030688	8	1	theme	G+C	703:705	arg1	content					707:713	The DNA G+C content	695:713	The DNA G+C content of TYRC17(T)	695:726	The DNA G+C content of TYRC17(T) was 53.9 mol%.
24030688	8	1	theme	G+C	703:705	arg1	%					740:740	53.9 mol%	732:740	53.9 mol%	732:740	The DNA G+C content of TYRC17(T) was 53.9 mol%.
24030688	13	2	theme	strain	1607:1612	arg1	T					1621:1621	T	1621:1621	T	1621:1621	On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas.
24030688	13	2	theme	strain	1607:1612	arg1	TYRC17					1614:1619	strain TYRC17	1607:1619	strain TYRC17(T)	1607:1622	On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas.
24030688	9	3	theme	16S	768:770	arg1	sequences					782:790	16S rRNA gene sequences	768:790	16S rRNA gene sequences	768:790	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	2	4	theme	halophilic	118:127	arg1	bacterium					167:175	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium	105:175	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T)	105:204	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T) was isolated from olive-processing effluents.
24030688	9	5	theme	Phylogenetic	743:754	arg1	analyses					756:763	Phylogenetic analyses	743:763	Phylogenetic analyses of 16S rRNA gene sequences	743:790	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	10	6	from	identities	1070:1079	arg1	particular					1084:1093	particular	1084:1093	particular	1084:1093	TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %).
24030688	11	7	theme	phylogenetic	1252:1263	arg1	position					1265:1272	the phylogenetic position	1248:1272	the phylogenetic position of TYRC17(T)	1248:1285	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T).
24030688	8	8	theme	TYRC17	718:723	arg1	content					707:713	The DNA G+C content	695:713	The DNA G+C content of TYRC17(T)	695:726	The DNA G+C content of TYRC17(T) was 53.9 mol%.
24030688	8	8	theme	TYRC17	718:723	arg1	%					740:740	53.9 mol%	732:740	53.9 mol%	732:740	The DNA G+C content of TYRC17(T) was 53.9 mol%.
24030688	4	9	from	°C	487:488	arg1	°C					467:468	4-50 °C	462:468	4-50 °C (optimally at 35 °C)	462:489	Growth occurred with 0-25 % (w/v) NaCl (optimum, 7 %), at pH 5-11 (optimum, pH 7.0) and at 4-50 °C (optimally at 35 °C).
24030688	9	10	contain	containing	895:904	arg2	sulfidaeris					916:926	Halomonas sulfidaeris	906:926	Halomonas sulfidaeris	906:926	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	9	10	contain	containing	895:904	arg2	H.					929:930	H.	929:930	H.	929:930	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	9	10	contain	containing	895:904	arg2	H.					958:959	H.	958:959	H.	958:959	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	9	10	contain	containing	895:904	arg2	H.					998:999	H.	998:999	H.	998:999	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	9	10	contain	containing	895:904	arg2	H.					977:978	H.	977:978	H.	977:978	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	9	10	contain	containing	895:904	arg2	H.					1017:1018	H.	1017:1018	H.	1017:1018	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	9	10	contain	containing	895:904	arg1	subgroup					886:893	the subgroup	882:893	the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia	882:1027	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	12	11	theme	21.6 	1400:1404	arg1	%					1405:1405	%	1405:1405	%	1405:1405	This, combined with the level of DNA-DNA hybridization between TYRC17(T) and its closest relatives ranging from 21.6 % to 48.4 %, indicated that TYRC17(T) did not represent any of these species.
24030688	1	12	theme	halophilic	42:51	arg1	bacterium					53:61	a moderately halophilic bacterium	29:61	a moderately halophilic bacterium	29:61	nov., a moderately halophilic bacterium isolated from olive-processing effluents.
24030688	1	12	theme	halophilic	42:51	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., a moderately halophilic bacterium isolated from olive-processing effluents.
24030688	10	13	theme	16S-rRNA	1052:1059	arg1	identities					1070:1079	high 16S-rRNA sequence identities	1047:1079	high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %)	1047:1142	TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %).
24030688	9	14	theme	gene	777:780	arg1	sequences					782:790	16S rRNA gene sequences	768:790	16S rRNA gene sequences	768:790	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	6	15	dep	C18 	606:609	arg1	C16 					633:636	C16 	633:636	C16 	633:636	The predominant fatty acids were C18 : 1ω7c, C16 : 1ω7c and C16 : 0.
24030688	6	15	dep	C18 	606:609	arg1	 1ω7c					623:627	 1ω7c	623:627	 1ω7c	623:627	The predominant fatty acids were C18 : 1ω7c, C16 : 1ω7c and C16 : 0.
24030688	6	15	dep	C18 	606:609	arg1	C16 					618:621	C16 	618:621	C16 	618:621	The predominant fatty acids were C18 : 1ω7c, C16 : 1ω7c and C16 : 0.
24030688	14	16	theme	olivaria	1695:1702	arg1	sp					1704:1705	The name Halomonas olivaria sp	1676:1705	The name Halomonas olivaria sp.	1676:1706	The name Halomonas olivaria sp.
24030688	9	17	dep	H.	958:959	arg1	zhanjiangensis					961:974	H. zhanjiangensis	958:974	H. zhanjiangensis	958:974	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	4	18	dep	optimum	411:417	arg1	%					422:422	7 %	420:422	7 %	420:422	Growth occurred with 0-25 % (w/v) NaCl (optimum, 7 %), at pH 5-11 (optimum, pH 7.0) and at 4-50 °C (optimally at 35 °C).
24030688	9	19	theme	sequences	782:790	arg1	analyses					756:763	Phylogenetic analyses	743:763	Phylogenetic analyses of 16S rRNA gene sequences	743:790	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	11	20	theme	rpoD	1209:1212	arg1	genes					1223:1227	the 23S rRNA, gyrB, rpoD and secA genes	1189:1227	the 23S rRNA, gyrB, rpoD and secA genes	1189:1227	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T).
24030688	5	21	theme	poly-β-hydroxyalkanoate	507:529	arg1	granules					531:538	poly-β-hydroxyalkanoate granules	507:538	poly-β-hydroxyalkanoate granules	507:538	It accumulated poly-β-hydroxyalkanoate granules and produced exopolysaccharides.
24030688	4	22	theme	%	397:397	arg1	NaCl					405:408	0-25 % (w/v) NaCl	392:408	0-25 % (w/v) NaCl (optimum, 7 %)	392:423	Growth occurred with 0-25 % (w/v) NaCl (optimum, 7 %), at pH 5-11 (optimum, pH 7.0) and at 4-50 °C (optimally at 35 °C).
24030688	4	22	theme	%	397:397	arg1	optimum					411:417	optimum	411:417	optimum	411:417	Growth occurred with 0-25 % (w/v) NaCl (optimum, 7 %), at pH 5-11 (optimum, pH 7.0) and at 4-50 °C (optimally at 35 °C).
24030688	13	23	theme	genomic	1550:1556	arg1	evidence					1575:1582	genomic and phylogenetic evidence	1550:1582	genomic and phylogenetic evidence	1550:1582	On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas.
24030688	9	24	dep	H.	998:999	arg1	boliviensis					1001:1011	H. boliviensis	998:1011	H. boliviensis	998:1011	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	13	25	theme	phylogenetic	1562:1573	arg1	evidence					1575:1582	genomic and phylogenetic evidence	1550:1582	genomic and phylogenetic evidence	1550:1582	On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas.
24030688	11	26	theme	23S	1193:1195	arg1	rRNA					1197:1200	23S rRNA	1193:1200	23S rRNA	1193:1200	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T).
24030688	9	27	theme	rRNA	772:775	arg1	sequences					782:790	16S rRNA gene sequences	768:790	16S rRNA gene sequences	768:790	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	14	28	theme	name	1680:1683	arg1	sp					1704:1705	The name Halomonas olivaria sp	1676:1705	The name Halomonas olivaria sp.	1676:1706	The name Halomonas olivaria sp.
24030688	10	29	theme	high	1047:1050	arg1	identities					1070:1079	high 16S-rRNA sequence identities	1047:1079	high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %)	1047:1142	TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %).
24030688	2	30	attach	isolated	210:217	arg1	effluents					241:249	olive-processing effluents	224:249	olive-processing effluents	224:249	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T) was isolated from olive-processing effluents.
24030688	2	30	attach	isolated	210:217	arg2	bacterium					167:175	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium	105:175	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T)	105:204	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T) was isolated from olive-processing effluents.
24030688	0	31	theme	olivaria	10:17	arg1	sp					19:20	Halomonas olivaria sp	0:20	Halomonas olivaria sp.	0:21	Halomonas olivaria sp.
24030688	13	32	theme	genotypic	1514:1522	arg1	characteristics					1524:1538	phenotypic and genotypic characteristics	1499:1538	phenotypic and genotypic characteristics	1499:1538	On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas.
24030688	9	33	theme	genus	844:848	arg1	Halomonas					850:858	the genus Halomonas	840:858	the genus Halomonas	840:858	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	1	34	theme	olive-processing	77:92	arg1	effluents					94:102	olive-processing effluents	77:102	olive-processing effluents	77:102	nov., a moderately halophilic bacterium isolated from olive-processing effluents.
24030688	0	35	theme	Halomonas	0:8	arg1	sp					19:20	Halomonas olivaria sp	0:20	Halomonas olivaria sp.	0:21	Halomonas olivaria sp.
24030688	7	36	theme	only	669:672	arg1	quinone					686:692	the only respiratory quinone	665:692	the only respiratory quinone	665:692	Ubiquinone 9 (Q-9) was the only respiratory quinone.
24030688	7	36	theme	only	669:672	arg1	Ubiquinone					642:651	Ubiquinone 9	642:653	Ubiquinone 9 (Q-9)	642:659	Ubiquinone 9 (Q-9) was the only respiratory quinone.
24030688	15	37	theme	type	1782:1785	arg1	strain					1787:1792	the type strain	1778:1792	the type strain	1778:1792	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	15	37	theme	type	1782:1785	arg1	nov.					1708:1711	nov.	1708:1711	nov.	1708:1711	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	8	38	theme	mol	737:739	arg1	content					707:713	The DNA G+C content	695:713	The DNA G+C content of TYRC17(T)	695:726	The DNA G+C content of TYRC17(T) was 53.9 mol%.
24030688	8	38	theme	mol	737:739	arg1	%					740:740	53.9 mol%	732:740	53.9 mol%	732:740	The DNA G+C content of TYRC17(T) was 53.9 mol%.
24030688	10	39	with	particular	1084:1093	arg1	species					1115:1121	the three last species	1100:1121	the three last species listed (99.4-99.5 %)	1100:1142	TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %).
24030688	10	39	with	particular	1084:1093	arg1	%					1141:1141	99.4-99.5 %	1131:1141	99.4-99.5 %	1131:1141	TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %).
24030688	7	40	theme	respiratory	674:684	arg1	quinone					686:692	the only respiratory quinone	665:692	the only respiratory quinone	665:692	Ubiquinone 9 (Q-9) was the only respiratory quinone.
24030688	7	40	theme	respiratory	674:684	arg1	Ubiquinone					642:651	Ubiquinone 9	642:653	Ubiquinone 9 (Q-9)	642:659	Ubiquinone 9 (Q-9) was the only respiratory quinone.
24030688	13	41	dep	characteristics	1524:1538	arg1	the					1486:1488	the	1486:1488	the	1486:1488	On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas.
24030688	13	41	dep	characteristics	1524:1538	arg1	basis					1490:1494	basis	1490:1494	basis	1490:1494	On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas.
24030688	13	42	theme	novel	1638:1642	arg1	species					1644:1650	a novel species	1636:1650	a novel species of the genus Halomonas	1636:1673	On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas.
24030688	13	43	theme	Halomonas	1665:1673	arg1	species					1644:1650	a novel species	1636:1650	a novel species of the genus Halomonas	1636:1673	On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas.
24030688	3	44	theme	motile	285:290	arg1	organism					256:263	The organism	252:263	The organism	252:263	The organism was a straight rod, motile by means of peritrichous flagella and able to respire both oxygen and nitrate.
24030688	3	44	theme	motile	285:290	arg1	rod					280:282	a straight rod	269:282	a straight rod	269:282	The organism was a straight rod, motile by means of peritrichous flagella and able to respire both oxygen and nitrate.
24030688	4	45	dep	°C	487:488	arg1	optimally					471:479	optimally	471:479	optimally	471:479	Growth occurred with 0-25 % (w/v) NaCl (optimum, 7 %), at pH 5-11 (optimum, pH 7.0) and at 4-50 °C (optimally at 35 °C).
24030688	9	46	theme	H.	943:944	arg1	variabilis					946:955	H. variabilis	943:955	H. variabilis	943:955	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	11	47	theme	secA	1218:1221	arg1	genes					1223:1227	the 23S rRNA, gyrB, rpoD and secA genes	1189:1227	the 23S rRNA, gyrB, rpoD and secA genes	1189:1227	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T).
24030688	12	48	theme	closest	1369:1375	arg1	relatives					1377:1385	its closest relatives	1365:1385	its closest relatives ranging from 21.6 % to 48.4 %	1365:1415	This, combined with the level of DNA-DNA hybridization between TYRC17(T) and its closest relatives ranging from 21.6 % to 48.4 %, indicated that TYRC17(T) did not represent any of these species.
24030688	11	49	theme	sequence	1158:1165	arg1	MLSA					1177:1180	MLSA	1177:1180	MLSA	1177:1180	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T).
24030688	11	49	theme	sequence	1158:1165	arg1	analysis					1167:1174	A multilocus sequence analysis	1145:1174	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes	1145:1227	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T).
24030688	3	50	theme	straight	271:278	arg1	organism					256:263	The organism	252:263	The organism	252:263	The organism was a straight rod, motile by means of peritrichous flagella and able to respire both oxygen and nitrate.
24030688	3	50	theme	straight	271:278	arg1	rod					280:282	a straight rod	269:282	a straight rod	269:282	The organism was a straight rod, motile by means of peritrichous flagella and able to respire both oxygen and nitrate.
24030688	10	51	theme	sequence	1061:1068	arg1	identities					1070:1079	high 16S-rRNA sequence identities	1047:1079	high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %)	1047:1142	TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %).
24030688	6	52	theme	fatty	589:593	arg1	C18 					606:609	C18 	606:609	C18 	606:609	The predominant fatty acids were C18 : 1ω7c, C16 : 1ω7c and C16 : 0.
24030688	6	52	theme	fatty	589:593	arg1	acids					595:599	The predominant fatty acids	573:599	The predominant fatty acids	573:599	The predominant fatty acids were C18 : 1ω7c, C16 : 1ω7c and C16 : 0.
24030688	6	53	dep	 1ω7c	623:627	arg1	 0					638:639	 0	638:639	 0	638:639	The predominant fatty acids were C18 : 1ω7c, C16 : 1ω7c and C16 : 0.
24030688	2	54	dep	halophilic	118:127	arg1	non-sporulating					151:165	non-sporulating	151:165	non-sporulating	151:165	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T) was isolated from olive-processing effluents.
24030688	2	54	dep	halophilic	118:127	arg1	Gram-stain-negative					130:148	Gram-stain-negative	130:148	Gram-stain-negative	130:148	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T) was isolated from olive-processing effluents.
24030688	2	55	theme	strain	189:194	arg1	TYRC17					196:201	strain TYRC17	189:201	strain TYRC17(T)	189:204	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T) was isolated from olive-processing effluents.
24030688	2	55	theme	strain	189:194	arg1	T					203:203	T	203:203	T	203:203	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T) was isolated from olive-processing effluents.
24030688	9	56	dep	H.	977:978	arg1	alkaliantarctica					980:995	H. alkaliantarctica	977:995	H. alkaliantarctica	977:995	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	14	57	theme	Halomonas	1685:1693	arg1	sp					1704:1705	The name Halomonas olivaria sp	1676:1705	The name Halomonas olivaria sp.	1676:1706	The name Halomonas olivaria sp.
24030688	12	58	theme	hybridization	1329:1341	arg1	level					1312:1316	the level	1308:1316	the level of DNA-DNA hybridization between TYRC17(T) and its closest relatives ranging from 21.6 % to 48.4 %	1308:1415	This, combined with the level of DNA-DNA hybridization between TYRC17(T) and its closest relatives ranging from 21.6 % to 48.4 %, indicated that TYRC17(T) did not represent any of these species.
24030688	4	59	dep	optimum	438:444	arg1	pH					447:448	pH 7.0	447:452	pH 7.0	447:452	Growth occurred with 0-25 % (w/v) NaCl (optimum, 7 %), at pH 5-11 (optimum, pH 7.0) and at 4-50 °C (optimally at 35 °C).
24030688	10	60	theme	last	1110:1113	arg1	species					1115:1121	the three last species	1100:1121	the three last species listed (99.4-99.5 %)	1100:1142	TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %).
24030688	10	60	theme	last	1110:1113	arg1	%					1141:1141	99.4-99.5 %	1131:1141	99.4-99.5 %	1131:1141	TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %).
24030688	2	61	theme	olive-processing	224:239	arg1	effluents					241:249	olive-processing effluents	224:249	olive-processing effluents	224:249	A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T) was isolated from olive-processing effluents.
24030688	11	62	theme	TYRC17	1277:1282	arg1	position					1265:1272	the phylogenetic position	1248:1272	the phylogenetic position of TYRC17(T)	1248:1285	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T).
24030688	11	63	theme	multilocus	1147:1156	arg1	MLSA					1177:1180	MLSA	1177:1180	MLSA	1177:1180	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T).
24030688	11	63	theme	multilocus	1147:1156	arg1	analysis					1167:1174	A multilocus sequence analysis	1145:1174	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes	1145:1227	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T).
24030688	3	64	theme	peritrichous	304:315	arg1	flagella					317:324	peritrichous flagella	304:324	peritrichous flagella	304:324	The organism was a straight rod, motile by means of peritrichous flagella and able to respire both oxygen and nitrate.
24030688	15	65	theme	T	1754:1754	arg1	T					1771:1771	T	1771:1771	T	1771:1771	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	15	65	theme	T	1754:1754	arg1	53850B					1764:1769	 = DSM 19074(T) = CCUG 53850B	1741:1769	 = DSM 19074(T) = CCUG 53850B(T)	1741:1772	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	15	66	theme	 = CCUG	1756:1762	arg1	T					1771:1771	T	1771:1771	T	1771:1771	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	15	66	theme	 = CCUG	1756:1762	arg1	53850B					1764:1769	 = DSM 19074(T) = CCUG 53850B	1741:1769	 = DSM 19074(T) = CCUG 53850B(T)	1741:1772	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	15	67	dep	TYRC17	1730:1735	arg1	T					1771:1771	T	1771:1771	T	1771:1771	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	15	67	dep	TYRC17	1730:1735	arg1	53850B					1764:1769	 = DSM 19074(T) = CCUG 53850B	1741:1769	 = DSM 19074(T) = CCUG 53850B(T)	1741:1772	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	6	68	theme	predominant	577:587	arg1	C18 					606:609	C18 	606:609	C18 	606:609	The predominant fatty acids were C18 : 1ω7c, C16 : 1ω7c and C16 : 0.
24030688	6	68	theme	predominant	577:587	arg1	acids					595:599	The predominant fatty acids	573:599	The predominant fatty acids	573:599	The predominant fatty acids were C18 : 1ω7c, C16 : 1ω7c and C16 : 0.
24030688	4	69	theme	w/v	400:402	arg1	NaCl					405:408	0-25 % (w/v) NaCl	392:408	0-25 % (w/v) NaCl (optimum, 7 %)	392:423	Growth occurred with 0-25 % (w/v) NaCl (optimum, 7 %), at pH 5-11 (optimum, pH 7.0) and at 4-50 °C (optimally at 35 °C).
24030688	4	69	theme	w/v	400:402	arg1	optimum					411:417	optimum	411:417	optimum	411:417	Growth occurred with 0-25 % (w/v) NaCl (optimum, 7 %), at pH 5-11 (optimum, pH 7.0) and at 4-50 °C (optimally at 35 °C).
24030688	15	70	theme	 = DSM	1741:1746	arg1	T					1771:1771	T	1771:1771	T	1771:1771	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	15	70	theme	 = DSM	1741:1746	arg1	53850B					1764:1769	 = DSM 19074(T) = CCUG 53850B	1741:1769	 = DSM 19074(T) = CCUG 53850B(T)	1741:1772	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	10	71	dep	listed	1123:1128	arg1	species					1115:1121	the three last species	1100:1121	the three last species listed (99.4-99.5 %)	1100:1142	TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %).
24030688	10	71	dep	listed	1123:1128	arg1	%					1141:1141	99.4-99.5 %	1131:1141	99.4-99.5 %	1131:1141	TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5 %).
24030688	12	72	theme	48.4 	1410:1414	arg1	%					1405:1405	%	1405:1405	%	1405:1405	This, combined with the level of DNA-DNA hybridization between TYRC17(T) and its closest relatives ranging from 21.6 % to 48.4 %, indicated that TYRC17(T) did not represent any of these species.
24030688	9	73	theme	Halomonas	850:858	arg1	member					830:835	a member	828:835	a member of the genus Halomonas	828:858	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	15	74	theme	19074	1748:1752	arg1	T					1771:1771	T	1771:1771	T	1771:1771	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	15	74	theme	19074	1748:1752	arg1	53850B					1764:1769	 = DSM 19074(T) = CCUG 53850B	1741:1769	 = DSM 19074(T) = CCUG 53850B(T)	1741:1772	nov. is proposed with TYRC17(T) ( = DSM 19074(T) = CCUG 53850B(T)) as the type strain.
24030688	13	75	theme	genus	1659:1663	arg1	Halomonas					1665:1673	the genus Halomonas	1655:1673	the genus Halomonas	1655:1673	On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas.
24030688	8	76	theme	DNA	699:701	arg1	content					707:713	The DNA G+C content	695:713	The DNA G+C content of TYRC17(T)	695:726	The DNA G+C content of TYRC17(T) was 53.9 mol%.
24030688	8	76	theme	DNA	699:701	arg1	%					740:740	53.9 mol%	732:740	53.9 mol%	732:740	The DNA G+C content of TYRC17(T) was 53.9 mol%.
24030688	11	77	theme	gyrB	1203:1206	arg1	genes					1223:1227	the 23S rRNA, gyrB, rpoD and secA genes	1189:1227	the 23S rRNA, gyrB, rpoD and secA genes	1189:1227	A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T).
24030688	3	78	theme	able	330:333	arg1	organism					256:263	The organism	252:263	The organism	252:263	The organism was a straight rod, motile by means of peritrichous flagella and able to respire both oxygen and nitrate.
24030688	3	78	theme	able	330:333	arg1	rod					280:282	a straight rod	269:282	a straight rod	269:282	The organism was a straight rod, motile by means of peritrichous flagella and able to respire both oxygen and nitrate.
24030688	12	79	dep	%	1405:1405	arg1	to					1407:1408	to	1407:1408	to	1407:1408	This, combined with the level of DNA-DNA hybridization between TYRC17(T) and its closest relatives ranging from 21.6 % to 48.4 %, indicated that TYRC17(T) did not represent any of these species.
24030688	9	80	dep	H.	1017:1018	arg1	neptunia					1020:1027	H. neptunia	1017:1027	H. neptunia	1017:1027	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	13	81	theme	phenotypic	1499:1508	arg1	characteristics					1524:1538	phenotypic and genotypic characteristics	1499:1538	phenotypic and genotypic characteristics	1499:1538	On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas.
24030688	9	82	dep	H.	929:930	arg1	titanicae					932:940	H. titanicae, H. variabilis	929:955	titanicae	932:940	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	9	82	dep	H.	929:930	arg1	variabilis					946:955	H. variabilis	943:955	H. variabilis	943:955	Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia.
24030688	12	83	theme	DNA-DNA	1321:1327	arg1	hybridization					1329:1341	DNA-DNA hybridization	1321:1341	DNA-DNA hybridization between TYRC17(T) and its closest relatives ranging from 21.6 % to 48.4 %	1321:1415	This, combined with the level of DNA-DNA hybridization between TYRC17(T) and its closest relatives ranging from 21.6 % to 48.4 %, indicated that TYRC17(T) did not represent any of these species.
28390282	5	0	theme	Enzymatic	605:613	arg1	hydrolysis					615:624	Enzymatic hydrolysis	605:624	Enzymatic hydrolysis of pretreated biomass	605:646	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	3	1	theme	composition	424:434	arg1	studies					476:482	the chemical composition, pretreatments and enzymatic hydrolysis studies	411:482	the chemical composition, pretreatments and enzymatic hydrolysis studies	411:482	In this study, biomass of 2years old S. grandiflora was selected for the chemical composition, pretreatments and enzymatic hydrolysis studies.
28390282	4	2	theme	wood	509:512	arg1	density					514:520	a wood density	507:520	a wood density of 3.89±0.01gmcm-3	507:539	The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin.
28390282	5	3	theme	pretreated	686:695	arg1	samples					697:703	phosphoric acid (H3PO4) pretreated samples	662:703	phosphoric acid (H3PO4) pretreated samples	662:703	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	5	4	dep	loadings	729:736	arg1	FPU					761:763	FPU	761:763	FPU	761:763	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	5	4	dep	loadings	729:736	arg1	Units					754:758	[1 Filter Paper Units	738:758	lower cellulase loadings [1 Filter Paper Units (FPU)]	713:765	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	5	5	theme	86	800:801	arg1	%					802:802	%	802:802	%	802:802	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	6	6	dep	transform	1088:1096	arg1	infrared					1098:1105	infrared	1098:1105	transform infrared spectroscopy (FTIR) analysis	1088:1134	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	5	7	theme	60FPU	855:859	arg1	biomass					880:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	6	8	theme	pretreatment	963:974	arg1	effectiveness					930:942	The effectiveness	926:942	The effectiveness of phosphoric acid pretreatment	926:974	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	5	9	theme	alkali	862:867	arg1	biomass					880:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	0	10	theme	growing	165:171	arg1	legume					178:183	A fast growing tree legume	158:183	A fast growing tree legume	158:183	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	6	11	theme	field	1023:1027	arg1	FE-SEM					1068:1073	FE-SEM	1068:1073	FE-SEM	1068:1073	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	6	11	theme	field	1023:1027	arg1	microscopy					1056:1065	field emission scanning electron microscopy	1023:1065	field emission scanning electron microscopy (FE-SEM)	1023:1074	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	2	12	theme	growing	237:243	arg1	legumes					250:256	the fast growing tree legumes	228:256	the fast growing tree legumes	228:256	is one of the fast growing tree legumes having the efficiency to produce around 50tha-1 above ground dry matters in a year.
28390282	5	13	theme	higher	828:833	arg1	biomass					880:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	0	14	theme	pretreating	86:96	arg1	acid					78:81	phosphoric acid	67:81	phosphoric acid	67:81	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	0	14	theme	pretreating	86:96	arg1	agents					98:103	pretreating agents	86:103	pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.	86:155	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	0	14	theme	pretreating	86:96	arg1	hydroxide					53:61	sodium hydroxide	46:61	sodium hydroxide	46:61	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	0	15	theme	fast	160:163	arg1	legume					178:183	A fast growing tree legume	158:183	A fast growing tree legume	158:183	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	2	16	theme	fast	232:235	arg1	legumes					250:256	the fast growing tree legumes	228:256	the fast growing tree legumes	228:256	is one of the fast growing tree legumes having the efficiency to produce around 50tha-1 above ground dry matters in a year.
28390282	5	17	theme	phosphoric	662:671	arg1	H3PO4					679:683	H3PO4	679:683	H3PO4	679:683	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	5	17	theme	phosphoric	662:671	arg1	acid					673:676	phosphoric acid	662:676	phosphoric acid (H3PO4) pretreated samples	662:703	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	3	18	theme	enzymatic	455:463	arg1	hydrolysis					465:474	enzymatic hydrolysis	455:474	enzymatic hydrolysis	455:474	In this study, biomass of 2years old S. grandiflora was selected for the chemical composition, pretreatments and enzymatic hydrolysis studies.
28390282	2	19	dep	one	221:223	arg1	having					258:263	having	258:263	having the efficiency to produce around 50tha-1 above ground dry matters in a year	258:339	is one of the fast growing tree legumes having the efficiency to produce around 50tha-1 above ground dry matters in a year.
28390282	6	20	theme	phosphoric	947:956	arg1	pretreatment					963:974	phosphoric acid pretreatment	947:974	phosphoric acid pretreatment	947:974	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	5	21	theme	acid	673:676	arg1	samples					697:703	phosphoric acid (H3PO4) pretreated samples	662:703	phosphoric acid (H3PO4) pretreated samples	662:703	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	4	22	with	biomass	494:500	arg1	density					514:520	a wood density	507:520	a wood density of 3.89±0.01gmcm-3	507:539	The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin.
28390282	0	23	theme	tree	173:176	arg1	legume					178:183	A fast growing tree legume	158:183	A fast growing tree legume	158:183	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	3	24	theme	hydrolysis	465:474	arg1	studies					476:482	the chemical composition, pretreatments and enzymatic hydrolysis studies	411:482	the chemical composition, pretreatments and enzymatic hydrolysis studies	411:482	In this study, biomass of 2years old S. grandiflora was selected for the chemical composition, pretreatments and enzymatic hydrolysis studies.
28390282	6	25	theme	acid	958:961	arg1	pretreatment					963:974	phosphoric acid pretreatment	947:974	phosphoric acid pretreatment	947:974	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	3	26	theme	2years	368:373	arg1	biomass					357:363	biomass	357:363	biomass of 2years old S. grandiflora	357:392	In this study, biomass of 2years old S. grandiflora was selected for the chemical composition, pretreatments and enzymatic hydrolysis studies.
28390282	5	27	theme	cellulase	719:727	arg1	loadings					729:736	lower cellulase loadings	713:736	lower cellulase loadings [1 Filter Paper Units (FPU)]	713:765	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	0	28	dep	legume	178:183	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.	0:155	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	5	29	theme	pretreated	629:638	arg1	biomass					640:646	pretreated biomass	629:646	pretreated biomass	629:646	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	4	30	theme	stem	489:492	arg1	biomass					494:500	The stem biomass	485:500	The stem biomass with a wood density of 3.89±0.01gmcm-3	485:539	The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin.
28390282	6	31	theme	spectroscopy	1107:1118	arg1	analysis					1127:1134	spectroscopy (FTIR) analysis	1107:1134	spectroscopy (FTIR) analysis	1107:1134	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	0	32	theme	glucose	24:30	arg1	release					32:38	efficient glucose release	14:38	efficient glucose release	14:38	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	5	33	theme	pretreated	869:878	arg1	biomass					880:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	2	34	theme	legumes	250:256	arg1	legumes					250:256	the fast growing tree legumes	228:256	the fast growing tree legumes	228:256	is one of the fast growing tree legumes having the efficiency to produce around 50tha-1 above ground dry matters in a year.
28390282	2	34	theme	legumes	250:256	arg1	one					221:223	one	221:223	one	221:223	is one of the fast growing tree legumes having the efficiency to produce around 50tha-1 above ground dry matters in a year.
28390282	5	35	theme	Paper	748:752	arg1	FPU					761:763	FPU	761:763	FPU	761:763	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	5	35	theme	Paper	748:752	arg1	Units					754:758	[1 Filter Paper Units	738:758	lower cellulase loadings [1 Filter Paper Units (FPU)]	713:765	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	0	36	theme	efficient	14:22	arg1	release					32:38	efficient glucose release	14:38	efficient glucose release	14:38	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	6	37	theme	emission	1029:1036	arg1	FE-SEM					1068:1073	FE-SEM	1068:1073	FE-SEM	1068:1073	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	6	37	theme	emission	1029:1036	arg1	microscopy					1056:1065	field emission scanning electron microscopy	1023:1065	field emission scanning electron microscopy (FE-SEM)	1023:1074	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	6	38	theme	electron	1047:1054	arg1	FE-SEM					1068:1073	FE-SEM	1068:1073	FE-SEM	1068:1073	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	6	38	theme	electron	1047:1054	arg1	microscopy					1056:1065	field emission scanning electron microscopy	1023:1065	field emission scanning electron microscopy (FE-SEM)	1023:1074	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	2	39	theme	tree	245:248	arg1	legumes					250:256	the fast growing tree legumes	228:256	the fast growing tree legumes	228:256	is one of the fast growing tree legumes having the efficiency to produce around 50tha-1 above ground dry matters in a year.
28390282	5	40	theme	lower	713:717	arg1	loadings					729:736	lower cellulase loadings	713:736	lower cellulase loadings [1 Filter Paper Units (FPU)]	713:765	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	6	41	dep	Fourier	1080:1086	arg1	transform					1088:1096	transform	1088:1096	transform infrared spectroscopy (FTIR) analysis	1088:1134	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	6	42	theme	X-ray	998:1002	arg1	XRD					1017:1019	XRD	1017:1019	XRD	1017:1019	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	6	42	theme	X-ray	998:1002	arg1	diffraction					1004:1014	X-ray diffraction	998:1014	X-ray diffraction (XRD)	998:1020	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	0	43	theme	grandiflora	134:144	arg1	Pers.					151:155	Sesbania grandiflora (L.) Pers.	125:155	Sesbania grandiflora (L.) Pers.	125:155	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	5	44	theme	biomass	640:646	arg1	hydrolysis					615:624	Enzymatic hydrolysis	605:624	Enzymatic hydrolysis of pretreated biomass	605:646	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	3	45	theme	old	375:377	arg1	2years					368:373	2years	368:373	2years old S. grandiflora	368:392	In this study, biomass of 2years old S. grandiflora was selected for the chemical composition, pretreatments and enzymatic hydrolysis studies.
28390282	0	46	theme	release	32:38	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.	0:155	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	0	47	theme	Sesbania	125:132	arg1	Pers.					151:155	Sesbania grandiflora (L.) Pers.	125:155	Sesbania grandiflora (L.) Pers.	125:155	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	5	48	theme	[1	738:739	arg1	FPU					761:763	FPU	761:763	FPU	761:763	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	5	48	theme	[1	738:739	arg1	Units					754:758	[1 Filter Paper Units	738:758	lower cellulase loadings [1 Filter Paper Units (FPU)]	713:765	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	1	49	theme	Sesbania	186:193	arg1	L.					208:209	L.	208:209	L.	208:209	Sesbania grandiflora (L.) Pers.
28390282	1	49	theme	Sesbania	186:193	arg1	grandiflora					195:205	Sesbania grandiflora	186:205	Sesbania grandiflora (L.) Pers.	186:216	Sesbania grandiflora (L.) Pers.
28390282	0	50	theme	L.	147:148	arg1	Pers.					151:155	Sesbania grandiflora (L.) Pers.	125:155	Sesbania grandiflora (L.) Pers.	125:155	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	5	51	theme	loadings	845:852	arg1	biomass					880:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	2	52	from	matters	323:329	arg1	year					336:339	a year	334:339	a year	334:339	is one of the fast growing tree legumes having the efficiency to produce around 50tha-1 above ground dry matters in a year.
28390282	5	53	theme	%	802:802	arg1	glucose					804:810	86% glucose	800:810	86% glucose	800:810	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	0	54	theme	sodium	46:51	arg1	acid					78:81	phosphoric acid	67:81	phosphoric acid	67:81	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	0	54	theme	sodium	46:51	arg1	agents					98:103	pretreating agents	86:103	pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.	86:155	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	0	54	theme	sodium	46:51	arg1	hydroxide					53:61	sodium hydroxide	46:61	sodium hydroxide	46:61	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	6	55	theme	scanning	1038:1045	arg1	FE-SEM					1068:1073	FE-SEM	1068:1073	FE-SEM	1068:1073	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	6	55	theme	scanning	1038:1045	arg1	microscopy					1056:1065	field emission scanning electron microscopy	1023:1065	field emission scanning electron microscopy (FE-SEM)	1023:1074	The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
28390282	5	56	theme	58	913:914	arg1	%					915:915	%	915:915	%	915:915	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	4	57	dep	%	558:558	arg1	cellulose					560:568	cellulose	560:568	about 38% cellulose	550:568	The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin.
28390282	3	58	theme	chemical	415:422	arg1	composition					424:434	chemical composition	415:434	chemical composition	415:434	In this study, biomass of 2years old S. grandiflora was selected for the chemical composition, pretreatments and enzymatic hydrolysis studies.
28390282	4	59	dep	%	573:573	arg1	hemicellulose					575:587	hemicellulose	575:587	12% hemicellulose	571:587	The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin.
28390282	0	60	theme	Pers.	151:155	arg1	biomass					114:120	the biomass	110:120	the biomass of Sesbania grandiflora (L.) Pers.	110:155	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	5	61	theme	%	915:915	arg1	glucose					917:923	58% glucose	913:923	58% glucose	913:923	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	3	62	dep	old	375:377	arg1	grandiflora					382:392	S. grandiflora	379:392	S. grandiflora	379:392	In this study, biomass of 2years old S. grandiflora was selected for the chemical composition, pretreatments and enzymatic hydrolysis studies.
28390282	4	63	dep	%	595:595	arg1	lignin					597:602	lignin	597:602	28% lignin	593:602	The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin.
28390282	5	64	theme	cellulase	835:843	arg1	biomass					880:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	higher cellulase loadings (60FPU) alkali pretreated biomass	828:886	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	4	65	theme	3.89±0.01gmcm-3	525:539	arg1	density					514:520	a wood density	507:520	a wood density of 3.89±0.01gmcm-3	507:539	The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin.
28390282	0	66	theme	phosphoric	67:76	arg1	acid					78:81	phosphoric acid	67:81	phosphoric acid	67:81	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	0	66	theme	phosphoric	67:76	arg1	agents					98:103	pretreating agents	86:103	pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.	86:155	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	0	66	theme	phosphoric	67:76	arg1	hydroxide					53:61	sodium hydroxide	46:61	sodium hydroxide	46:61	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	3	67	theme	S.	379:380	arg1	grandiflora					382:392	S. grandiflora	379:392	S. grandiflora	379:392	In this study, biomass of 2years old S. grandiflora was selected for the chemical composition, pretreatments and enzymatic hydrolysis studies.
28390282	5	68	theme	Filter	741:746	arg1	FPU					761:763	FPU	761:763	FPU	761:763	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	5	68	theme	Filter	741:746	arg1	Units					754:758	[1 Filter Paper Units	738:758	lower cellulase loadings [1 Filter Paper Units (FPU)]	713:765	Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose.
28390282	3	69	theme	pretreatments	437:449	arg1	studies					476:482	the chemical composition, pretreatments and enzymatic hydrolysis studies	411:482	the chemical composition, pretreatments and enzymatic hydrolysis studies	411:482	In this study, biomass of 2years old S. grandiflora was selected for the chemical composition, pretreatments and enzymatic hydrolysis studies.
28390282	4	70	contain	contains	541:548	arg2	%					595:595	28%	593:595	28% lignin	593:602	The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin.
28390282	4	70	contain	contains	541:548	arg1	biomass					494:500	The stem biomass	485:500	The stem biomass with a wood density of 3.89±0.01gmcm-3	485:539	The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin.
28390282	4	70	contain	contains	541:548	arg2	%					573:573	12%	571:573	12% hemicellulose	571:587	The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin.
28390282	4	70	contain	contains	541:548	arg2	%					558:558	about 38%	550:558	about 38% cellulose	550:568	The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin.
28390282	0	71	from	biomass	114:120	arg1	acid					78:81	phosphoric acid	67:81	phosphoric acid	67:81	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	0	71	from	biomass	114:120	arg1	agents					98:103	pretreating agents	86:103	pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.	86:155	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
28390282	0	71	from	biomass	114:120	arg1	hydroxide					53:61	sodium hydroxide	46:61	sodium hydroxide	46:61	Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
29267210	2	0	from	bamboo	386:391	arg1	preparation					357:367	the LCC preparation	349:367	the LCC preparation from 2-month-old bamboo (L₂)	349:396	The results have shown that the LCC preparation from 2-month-old bamboo (L₂) exhibited a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24).
29267210	1	1	theme	bamboo	234:239	arg1	culms					266:270	24-month-old crude bamboo (Neosinocalamus affinis) culms	215:270	24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH)	215:295	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	7	2	theme	β-O-4	1251:1255	arg1	linkages					1263:1270	β-O-4 ether linkages	1251:1270	β-O-4 ether linkages	1251:1270	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	5	3	theme	ester	1056:1060	arg1	bonds					1066:1070	ester LCC bonds	1056:1070	ester LCC bonds	1056:1070	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	2	4	from	bamboo	489:494	arg1	preparation					455:465	the LCC preparation	447:465	the LCC preparation from the 24-month-old bamboo (L24)	447:500	The results have shown that the LCC preparation from 2-month-old bamboo (L₂) exhibited a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24).
29267210	4	5	from	content	752:758	arg1	bamboo					803:808	the mature bamboo	792:808	the mature bamboo	792:808	The content of the S lignin units of LCC in the mature bamboo was always higher than in the young bamboo.
29267210	3	6	theme	quantum	598:604	arg1	spectra					630:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	7	7	from	L₂	1310:1311	arg1	present					1299:1305	present	1299:1305	present	1299:1305	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	5	8	theme	LCC	1062:1064	arg1	bonds					1066:1070	ester LCC bonds	1056:1070	ester LCC bonds	1056:1070	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	7	9	theme	ether	1257:1261	arg1	linkages					1263:1270	β-O-4 ether linkages	1251:1270	β-O-4 ether linkages	1251:1270	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	6	10	theme	linkage	1125:1131	arg1	type					1113:1116	the main type	1104:1116	the main type of LCC linkage in bamboo LCCs	1104:1146	Lignin-xylan was the main type of LCC linkage in bamboo LCCs.
29267210	6	10	theme	linkage	1125:1131	arg1	Lignin-xylan					1087:1098	Lignin-xylan	1087:1098	Lignin-xylan	1087:1098	Lignin-xylan was the main type of LCC linkage in bamboo LCCs.
29267210	5	11	theme	linkages	939:946	arg1	contents					900:907	the contents	896:907	the contents of phenyl glycoside and ether linkages in the L24 preparation	896:969	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	5	11	theme	linkages	939:946	arg1	higher					976:981	higher	976:981	higher	976:981	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	1	12	theme	Neosinocalamus	242:255	arg1	culms					266:270	24-month-old crude bamboo (Neosinocalamus affinis) culms	215:270	24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH)	215:295	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	0	13	theme	24-Month-Old	84:95	arg1	Bamboo					97:102	24-Month-Old Bamboo	84:102	24-Month-Old Bamboo (Neosinocalamus affinis)	84:127	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).
29267210	0	13	theme	24-Month-Old	84:95	arg1	affinis					120:126	Neosinocalamus affinis	105:126	Neosinocalamus affinis	105:126	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).
29267210	5	14	theme	phenyl	912:917	arg1	glycoside					919:927	phenyl glycoside	912:927	phenyl glycoside	912:927	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	1	15	theme	affinis	257:263	arg1	culms					266:270	24-month-old crude bamboo (Neosinocalamus affinis) culms	215:270	24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH)	215:295	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	0	16	theme	Neosinocalamus	105:118	arg1	Bamboo					97:102	24-Month-Old Bamboo	84:102	24-Month-Old Bamboo (Neosinocalamus affinis)	84:127	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).
29267210	0	16	theme	Neosinocalamus	105:118	arg1	affinis					120:126	Neosinocalamus affinis	105:126	Neosinocalamus affinis	105:126	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).
29267210	7	17	theme	Lignin-lignin	1149:1161	arg1	linkages					1163:1170	Lignin-lignin linkages	1149:1170	Lignin-lignin linkages in the LCC preparations	1149:1194	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	4	18	theme	mature	796:801	arg1	bamboo					803:808	the mature bamboo	792:808	the mature bamboo	792:808	The content of the S lignin units of LCC in the mature bamboo was always higher than in the young bamboo.
29267210	7	19	from	linkages	1163:1170	arg1	preparations					1183:1194	the LCC preparations	1175:1194	the LCC preparations	1175:1194	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	5	20	theme	glycoside	919:927	arg1	contents					900:907	the contents	896:907	the contents of phenyl glycoside and ether linkages in the L24 preparation	896:969	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	5	20	theme	glycoside	919:927	arg1	higher					976:981	higher	976:981	higher	976:981	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	3	21	dep	transform	533:541	arg1	infrared					543:550	infrared	543:550	transform infrared spectroscopy (FT-IR)	533:571	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	3	22	theme	LCC	665:667	arg1	preparations					669:680	the LCC preparations	661:680	the LCC preparations	661:680	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	2	23	theme	LCC	353:355	arg1	preparation					357:367	the LCC preparation	349:367	the LCC preparation from 2-month-old bamboo (L₂)	349:396	The results have shown that the LCC preparation from 2-month-old bamboo (L₂) exhibited a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24).
29267210	3	24	theme	G-S-H	717:721	arg1	lignin-type					723:733	G-S-H lignin-type	717:733	G-S-H lignin-type	717:733	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	3	25	theme	2D-HSQC	617:623	arg1	spectra					630:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	0	26	theme	Structural	0:9	arg1	Differences					11:21	Structural Differences	0:21	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).	0:128	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).
29267210	6	27	theme	main	1108:1111	arg1	type					1113:1116	the main type	1104:1116	the main type of LCC linkage in bamboo LCCs	1104:1146	Lignin-xylan was the main type of LCC linkage in bamboo LCCs.
29267210	6	27	theme	main	1108:1111	arg1	Lignin-xylan					1087:1098	Lignin-xylan	1087:1098	Lignin-xylan	1087:1098	Lignin-xylan was the main type of LCC linkage in bamboo LCCs.
29267210	3	28	with	lignin-type	723:733	arg1	G>S>>H					740:745	G>S>>H	740:745	G>S>>H	740:745	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	7	29	theme	β-1	1218:1220	arg1	carbon-to-carbon					1222:1237	β-1 carbon-to-carbon	1218:1237	β-1 carbon-to-carbon	1218:1237	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	6	30	from	type	1113:1116	arg1	LCCs					1143:1146	bamboo LCCs	1136:1146	bamboo LCCs	1136:1146	Lignin-xylan was the main type of LCC linkage in bamboo LCCs.
29267210	1	31	theme	culms	266:270	arg1	lignin					198:203	milled wood lignin	186:203	milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH)	186:295	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	6	32	theme	LCC	1121:1123	arg1	linkage					1125:1131	LCC linkage	1121:1131	LCC linkage	1121:1131	Lignin-xylan was the main type of LCC linkage in bamboo LCCs.
29267210	3	33	theme	Further	503:509	arg1	studies					511:517	Further studies	503:517	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses	503:645	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	6	34	theme	bamboo	1136:1141	arg1	LCCs					1143:1146	bamboo LCCs	1136:1146	bamboo LCCs	1136:1146	Lignin-xylan was the main type of LCC linkage in bamboo LCCs.
29267210	1	35	theme	milled	186:191	arg1	lignin					198:203	milled wood lignin	186:203	milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH)	186:295	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	3	36	theme	coherence	606:614	arg1	spectra					630:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	3	37	dep	Fourier	525:531	arg1	transform					533:541	transform	533:541	transform infrared spectroscopy (FT-IR)	533:571	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	3	37	dep	Fourier	525:531	arg1	analyses					638:645	analyses	638:645	analyses	638:645	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	1	38	theme	wood	193:196	arg1	lignin					198:203	milled wood lignin	186:203	milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH)	186:295	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	1	39	theme	acetic	278:283	arg1	AcOH					291:294	AcOH	291:294	AcOH	291:294	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	1	39	theme	acetic	278:283	arg1	acid					285:288	acetic acid	278:288	acetic acid (AcOH)	278:295	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	0	40	from	2-	77:78	arg1	Complexes					55:63	the Lignin-Carbohydrate Complexes	31:63	the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis)	31:127	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).
29267210	0	40	from	2-	77:78	arg1	LCCs					66:69	LCCs	66:69	LCCs	66:69	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).
29267210	5	41	theme	L24	955:957	arg1	preparation					959:969	the L24 preparation	951:969	the L24 preparation	951:969	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	7	42	theme	β-1	1277:1279	arg1	linkages					1281:1288	β-1 linkages	1277:1288	β-1 linkages	1277:1288	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	2	43	theme	2-month-old	374:384	arg1	L₂					394:395	L₂	394:395	L₂	394:395	The results have shown that the LCC preparation from 2-month-old bamboo (L₂) exhibited a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24).
29267210	2	43	theme	2-month-old	374:384	arg1	bamboo					386:391	2-month-old bamboo	374:391	2-month-old bamboo (L₂)	374:396	The results have shown that the LCC preparation from 2-month-old bamboo (L₂) exhibited a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24).
29267210	0	44	theme	Lignin-Carbohydrate	35:53	arg1	Complexes					55:63	the Lignin-Carbohydrate Complexes	31:63	the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis)	31:127	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).
29267210	0	44	theme	Lignin-Carbohydrate	35:53	arg1	LCCs					66:69	LCCs	66:69	LCCs	66:69	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).
29267210	5	45	theme	bonds	1066:1070	arg1	relationship					1040:1051	a reverse relationship	1030:1051	a reverse relationship of ester LCC bonds	1030:1070	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	2	46	theme	molecular	425:433	arg1	weight					435:440	a slightly lower molecular weight	408:440	a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24)	408:500	The results have shown that the LCC preparation from 2-month-old bamboo (L₂) exhibited a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24).
29267210	3	47	theme	single	591:596	arg1	spectra					630:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	4	48	theme	units	776:780	arg1	higher					821:826	higher	821:826	higher	821:826	The content of the S lignin units of LCC in the mature bamboo was always higher than in the young bamboo.
29267210	4	48	theme	units	776:780	arg1	content					752:758	The content	748:758	The content of the S lignin units of LCC in the mature bamboo	748:808	The content of the S lignin units of LCC in the mature bamboo was always higher than in the young bamboo.
29267210	7	49	located	present	1299:1305	arg1	L₂					1310:1311	L₂	1310:1311	L₂	1310:1311	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	7	49	located	present	1299:1305	arg2	linkages					1281:1288	β-1 linkages	1277:1288	β-1 linkages	1277:1288	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	2	50	theme	lower	419:423	arg1	weight					435:440	a slightly lower molecular weight	408:440	a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24)	408:500	The results have shown that the LCC preparation from 2-month-old bamboo (L₂) exhibited a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24).
29267210	4	51	theme	young	840:844	arg1	bamboo					846:851	the young bamboo	836:851	the young bamboo	836:851	The content of the S lignin units of LCC in the mature bamboo was always higher than in the young bamboo.
29267210	1	52	attach	isolated	172:179	arg2	LCC					163:165	LCC	163:165	LCC	163:165	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	1	52	attach	isolated	172:179	arg2	complex					154:160	The lignin-carbohydrate complex	130:160	The lignin-carbohydrate complex (LCC)	130:166	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	1	52	attach	isolated	172:179	arg1	lignin					198:203	milled wood lignin	186:203	milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH)	186:295	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	2	53	theme	24-month-old	476:487	arg1	L24					497:499	L24	497:499	L24	497:499	The results have shown that the LCC preparation from 2-month-old bamboo (L₂) exhibited a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24).
29267210	2	53	theme	24-month-old	476:487	arg1	bamboo					489:494	the 24-month-old bamboo	472:494	the 24-month-old bamboo (L24)	472:500	The results have shown that the LCC preparation from 2-month-old bamboo (L₂) exhibited a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24).
29267210	7	54	theme	LCC	1179:1181	arg1	preparations					1183:1194	the LCC preparations	1175:1194	the LCC preparations	1175:1194	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	5	55	theme	reverse	1032:1038	arg1	relationship					1040:1051	a reverse relationship	1030:1051	a reverse relationship of ester LCC bonds	1030:1070	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	3	56	theme	NMR	626:628	arg1	spectra					630:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	4	57	theme	LCC	785:787	arg1	units					776:780	the S lignin units	763:780	the S lignin units of LCC	763:787	The content of the S lignin units of LCC in the mature bamboo was always higher than in the young bamboo.
29267210	7	58	attach	present	1299:1305	arg1	L₂					1310:1311	L₂	1310:1311	L₂	1310:1311	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	7	58	attach	present	1299:1305	arg2	linkages					1281:1288	β-1 linkages	1277:1288	β-1 linkages	1277:1288	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	5	59	theme	ether	933:937	arg1	linkages					939:946	ether linkages	933:946	ether linkages	933:946	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	5	60	theme	sugar	868:872	arg1	analysis					886:893	sugar composition analysis	868:893	sugar composition analysis	868:893	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	5	61	from	contents	900:907	arg1	preparation					959:969	the L24 preparation	951:969	the L24 preparation	951:969	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	1	62	theme	2-	208:209	arg1	lignin					198:203	milled wood lignin	186:203	milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH)	186:295	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	0	63	from	Bamboo	97:102	arg1	Complexes					55:63	the Lignin-Carbohydrate Complexes	31:63	the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis)	31:127	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).
29267210	0	63	from	Bamboo	97:102	arg1	LCCs					66:69	LCCs	66:69	LCCs	66:69	Structural Differences between the Lignin-Carbohydrate Complexes (LCCs) from 2- and 24-Month-Old Bamboo (Neosinocalamus affinis).
29267210	3	64	theme	heteronuclear	577:589	arg1	spectra					630:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	heteronuclear single quantum coherence (2D-HSQC) NMR spectra	577:636	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	5	65	theme	composition	874:884	arg1	analysis					886:893	sugar composition analysis	868:893	sugar composition analysis	868:893	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	5	66	theme	L₂	995:996	arg1	preparation					998:1008	the L₂ preparation	991:1008	the L₂ preparation	991:1008	Combined with sugar composition analysis, the contents of phenyl glycoside and ether linkages in the L24 preparation were higher than in the L₂ preparation; however, there was a reverse relationship of ester LCC bonds in L₂ and L24.
29267210	4	67	theme	lignin	769:774	arg1	units					776:780	the S lignin units	763:780	the S lignin units of LCC	763:787	The content of the S lignin units of LCC in the mature bamboo was always higher than in the young bamboo.
29267210	1	68	theme	lignin-carbohydrate	134:152	arg1	complex					154:160	The lignin-carbohydrate complex	130:160	The lignin-carbohydrate complex (LCC)	130:166	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	1	68	theme	lignin-carbohydrate	134:152	arg1	LCC					163:165	LCC	163:165	LCC	163:165	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	3	69	with	glucuronoarabinoxylan	691:711	arg1	G>S>>H					740:745	G>S>>H	740:745	G>S>>H	740:745	Further studies using Fourier transform infrared spectroscopy (FT-IR) and heteronuclear single quantum coherence (2D-HSQC) NMR spectra analyses indicate that the LCC preparations included glucuronoarabinoxylan and G-S-H lignin-type with G>S>>H.
29267210	1	70	theme	24-month-old	215:226	arg1	culms					266:270	24-month-old crude bamboo (Neosinocalamus affinis) culms	215:270	24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH)	215:295	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
29267210	2	71	theme	LCC	451:453	arg1	preparation					455:465	the LCC preparation	447:465	the LCC preparation from the 24-month-old bamboo (L24)	447:500	The results have shown that the LCC preparation from 2-month-old bamboo (L₂) exhibited a slightly lower molecular weight than the LCC preparation from the 24-month-old bamboo (L24).
29267210	4	72	theme	S	767:767	arg1	units					776:780	the S lignin units	763:780	the S lignin units of LCC	763:787	The content of the S lignin units of LCC in the mature bamboo was always higher than in the young bamboo.
29267210	7	73	from	present	1299:1305	arg1	L₂					1310:1311	L₂	1310:1311	L₂	1310:1311	Lignin-lignin linkages in the LCC preparations included β-β, β-5 and β-1 carbon-to-carbon, as well as β-O-4 ether linkages, but β-1 linkages were not present in L₂.
29267210	1	74	theme	crude	228:232	arg1	culms					266:270	24-month-old crude bamboo (Neosinocalamus affinis) culms	215:270	24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH)	215:295	The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized.
26951372	4	0	theme	ABA	581:583	arg1	excess					566:571	large excess	560:571	large excess of free ABA	560:583	Calculation of free versus antibody-bound ABA reveals large excess of free ABA, increasing signficantly in caryopses from 10 days after fertilization.
26951372	13	1	theme	high	2022:2025	arg1	amounts					2031:2037	unnaturally high ABA amounts	2010:2037	unnaturally high ABA amounts	2010:2037	Such compensation implicates the enormous physiological and metabolic flexibilities of barley grains to adjust effects of unnaturally high ABA amounts in order to ensure and maintain proper grain development.
26951372	13	2	theme	grains	1982:1987	arg1	flexibilities					1958:1970	the enormous physiological and metabolic flexibilities	1917:1970	the enormous physiological and metabolic flexibilities of barley grains	1917:1987	Such compensation implicates the enormous physiological and metabolic flexibilities of barley grains to adjust effects of unnaturally high ABA amounts in order to ensure and maintain proper grain development.
26951372	5	3	theme	transcript	672:681	arg1	profiling					683:691	transcript profiling	672:691	transcript profiling	672:691	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	13	4	theme	metabolic	1948:1956	arg1	flexibilities					1958:1970	the enormous physiological and metabolic flexibilities	1917:1970	the enormous physiological and metabolic flexibilities of barley grains	1917:1987	Such compensation implicates the enormous physiological and metabolic flexibilities of barley grains to adjust effects of unnaturally high ABA amounts in order to ensure and maintain proper grain development.
26951372	3	5	with	grains	370:375	arg1	caryopses					408:416	the resulting transgenic caryopses	383:416	the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA	383:503	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	4	6	from	days	631:634	arg1	caryopses					613:621	caryopses	613:621	caryopses from 10 days after fertilization	613:654	Calculation of free versus antibody-bound ABA reveals large excess of free ABA, increasing signficantly in caryopses from 10 days after fertilization.
26951372	3	7	with	expression	459:468	arg1	accumulation					485:496	increased accumulation	475:496	increased accumulation of ABA	475:503	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	9	8	theme	ABA	1365:1367	arg1	accumulation					1369:1380	precocious ABA accumulation	1354:1380	precocious ABA accumulation that generates an integrated response of stress and maturation	1354:1443	Conclusively, ABA immunomodulation results in precocious ABA accumulation that generates an integrated response of stress and maturation.
26951372	7	9	theme	abiotic	1099:1105	arg1	stresses					1107:1114	biotic and abiotic stresses	1088:1114	biotic and abiotic stresses	1088:1114	Anti-ABA grains display broad constitutive gene induction related to biotic and abiotic stresses.
26951372	1	10	theme	filling	210:216	arg1	phase					218:222	the seed filling phase	201:222	the seed filling phase	201:222	Abscisic acid (ABA) accumulates in seeds during the transition to the seed filling phase.
26951372	13	11	theme	physiological	1930:1942	arg1	flexibilities					1958:1970	the enormous physiological and metabolic flexibilities	1917:1970	the enormous physiological and metabolic flexibilities of barley grains	1917:1987	Such compensation implicates the enormous physiological and metabolic flexibilities of barley grains to adjust effects of unnaturally high ABA amounts in order to ensure and maintain proper grain development.
26951372	7	12	theme	biotic	1088:1093	arg1	stresses					1107:1114	biotic and abiotic stresses	1088:1114	biotic and abiotic stresses	1088:1114	Anti-ABA grains display broad constitutive gene induction related to biotic and abiotic stresses.
26951372	1	13	theme	Abscisic	135:142	arg1	acid					144:147	Abscisic acid	135:147	Abscisic acid (ABA)	135:153	Abscisic acid (ABA) accumulates in seeds during the transition to the seed filling phase.
26951372	1	13	theme	Abscisic	135:142	arg1	ABA					150:152	ABA	150:152	ABA	150:152	Abscisic acid (ABA) accumulates in seeds during the transition to the seed filling phase.
26951372	7	14	theme	related	1077:1083	arg1	induction					1067:1075	broad constitutive gene induction	1043:1075	broad constitutive gene induction related to biotic and abiotic stresses	1043:1114	Anti-ABA grains display broad constitutive gene induction related to biotic and abiotic stresses.
26951372	5	15	theme	transduction	876:887	arg1	up-regulation					780:792	transcriptional up-regulation	764:792	transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction	764:887	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	7	16	theme	gene	1062:1065	arg1	induction					1067:1075	broad constitutive gene induction	1043:1075	broad constitutive gene induction related to biotic and abiotic stresses	1043:1114	Anti-ABA grains display broad constitutive gene induction related to biotic and abiotic stresses.
26951372	12	17	theme	dry	1860:1862	arg1	weight					1864:1869	nearly unchanged dry weight	1843:1869	nearly unchanged dry weight	1843:1869	This indicates that anti-ABA caryopses induce specific mechanisms to desensitize ABA signalling efficiently, which finally yields mature grains with nearly unchanged dry weight and composition.
26951372	12	18	theme	anti-ABA	1714:1721	arg1	caryopses					1723:1731	anti-ABA caryopses	1714:1731	anti-ABA caryopses	1714:1731	This indicates that anti-ABA caryopses induce specific mechanisms to desensitize ABA signalling efficiently, which finally yields mature grains with nearly unchanged dry weight and composition.
26951372	5	19	dep	expose	712:717	arg1	triggered					719:727	triggered	719:727	triggered	719:727	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	5	19	dep	expose	712:717	arg1	enhanced					733:740	enhanced	733:740	enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction	733:887	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	13	20	theme	grain	2078:2082	arg1	development					2084:2094	proper grain development	2071:2094	proper grain development	2071:2094	Such compensation implicates the enormous physiological and metabolic flexibilities of barley grains to adjust effects of unnaturally high ABA amounts in order to ensure and maintain proper grain development.
26951372	0	21	theme	precocious	77:86	arg1	maturation					88:97	precocious maturation	77:97	precocious maturation	77:97	Increasing abscisic acid levels by immunomodulation in barley grains induces precocious maturation without changing grain composition.
26951372	9	22	theme	stress	1423:1428	arg1	response					1411:1418	an integrated response	1397:1418	an integrated response of stress and maturation	1397:1443	Conclusively, ABA immunomodulation results in precocious ABA accumulation that generates an integrated response of stress and maturation.
26951372	7	23	theme	broad	1043:1047	arg1	induction					1067:1075	broad constitutive gene induction	1043:1075	broad constitutive gene induction related to biotic and abiotic stresses	1043:1114	Anti-ABA grains display broad constitutive gene induction related to biotic and abiotic stresses.
26951372	3	24	theme	ABA	349:351	arg1	status					353:358	the ABA status	345:358	the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA	345:503	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	13	25	theme	Such	1888:1891	arg1	compensation					1893:1904	Such compensation	1888:1904	Such compensation	1888:1904	Such compensation implicates the enormous physiological and metabolic flexibilities of barley grains to adjust effects of unnaturally high ABA amounts in order to ensure and maintain proper grain development.
26951372	9	26	theme	maturation	1434:1443	arg1	response					1411:1418	an integrated response	1397:1418	an integrated response of stress and maturation	1397:1443	Conclusively, ABA immunomodulation results in precocious ABA accumulation that generates an integrated response of stress and maturation.
26951372	3	27	used	used	331:334	arg2	Immunomodulation					310:325	Immunomodulation	310:325	Immunomodulation	310:325	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	11	28	theme	grain	1589:1593	arg1	weight					1595:1600	mature grain weight	1582:1600	mature grain weight	1582:1600	Finally, mature grain weight and composition are unchanged in anti-ABA plants, although germination is somewhat delayed.
26951372	5	29	theme	transcriptional	764:778	arg1	up-regulation					780:792	transcriptional up-regulation	764:792	transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction	764:887	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	5	30	theme	ABA-related	857:867	arg1	transduction					876:887	ABA-related signal transduction	857:887	ABA-related signal transduction	857:887	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	2	31	theme	seed	238:241	arg1	maturation					243:252	seed maturation	238:252	seed maturation	238:252	ABA triggers seed maturation, storage activity, and stress signalling and tolerance.
26951372	13	32	theme	amounts	2031:2037	arg1	effects					1999:2005	effects	1999:2005	effects of unnaturally high ABA amounts	1999:2037	Such compensation implicates the enormous physiological and metabolic flexibilities of barley grains to adjust effects of unnaturally high ABA amounts in order to ensure and maintain proper grain development.
26951372	3	33	theme	gene	454:457	arg1	expression					459:468	the anti-ABA antibody gene expression	432:468	the anti-ABA antibody gene expression with increased accumulation of ABA	432:503	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	0	34	theme	acid	20:23	arg1	levels					25:30	abscisic acid levels	11:30	abscisic acid levels	11:30	Increasing abscisic acid levels by immunomodulation in barley grains induces precocious maturation without changing grain composition.
26951372	11	35	from	plants	1644:1649	arg1	unchanged					1622:1630	unchanged	1622:1630	unchanged	1622:1630	Finally, mature grain weight and composition are unchanged in anti-ABA plants, although germination is somewhat delayed.
26951372	3	36	theme	resulting	387:395	arg1	caryopses					408:416	the resulting transgenic caryopses	383:416	the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA	383:503	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	3	37	theme	anti-ABA	436:443	arg1	expression					459:468	the anti-ABA antibody gene expression	432:468	the anti-ABA antibody gene expression with increased accumulation of ABA	432:503	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	5	38	theme	storage	827:833	arg1	synthesis					843:851	storage protein synthesis	827:851	storage protein synthesis	827:851	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	4	39	theme	free	521:524	arg1	ABA					548:550	free versus antibody-bound ABA	521:550	free versus antibody-bound ABA	521:550	Calculation of free versus antibody-bound ABA reveals large excess of free ABA, increasing signficantly in caryopses from 10 days after fertilization.
26951372	5	40	theme	synthesis	843:851	arg1	up-regulation					780:792	transcriptional up-regulation	764:792	transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction	764:887	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	6	41	theme	enhanced	896:903	arg1	ABA					905:907	enhanced ABA	896:907	enhanced ABA during transition phases	896:932	Thus, enhanced ABA during transition phases induces precocious maturation but negatively interferes with growth and development.
26951372	4	42	theme	antibody-bound	533:546	arg1	ABA					548:550	free versus antibody-bound ABA	521:550	free versus antibody-bound ABA	521:550	Calculation of free versus antibody-bound ABA reveals large excess of free ABA, increasing signficantly in caryopses from 10 days after fertilization.
26951372	10	43	theme	ABA	1460:1462	arg1	signalling					1464:1473	ABA signalling	1460:1473	ABA signalling	1460:1473	Repression of ABA signalling, occurring in anti-ABA grains, potentially antagonizes effects caused by overshooting production.
26951372	3	44	theme	antibody	445:452	arg1	expression					459:468	the anti-ABA antibody gene expression	432:468	the anti-ABA antibody gene expression with increased accumulation of ABA	432:503	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	5	45	from	profiling	683:691	arg1	grains					705:710	anti-ABA grains	696:710	anti-ABA grains	696:710	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	5	46	theme	protein	835:841	arg1	synthesis					843:851	storage protein synthesis	827:851	storage protein synthesis	827:851	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	3	47	theme	ABA	501:503	arg1	accumulation					485:496	increased accumulation	475:496	increased accumulation of ABA	475:503	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	4	48	theme	free	576:579	arg1	ABA					581:583	free ABA	576:583	free ABA	576:583	Calculation of free versus antibody-bound ABA reveals large excess of free ABA, increasing signficantly in caryopses from 10 days after fertilization.
26951372	13	49	theme	barley	1975:1980	arg1	grains					1982:1987	barley grains	1975:1987	barley grains	1975:1987	Such compensation implicates the enormous physiological and metabolic flexibilities of barley grains to adjust effects of unnaturally high ABA amounts in order to ensure and maintain proper grain development.
26951372	12	50	dep	induce	1733:1738	arg1	yields					1817:1822	yields	1817:1822	yields mature grains with nearly unchanged dry weight and composition	1817:1885	This indicates that anti-ABA caryopses induce specific mechanisms to desensitize ABA signalling efficiently, which finally yields mature grains with nearly unchanged dry weight and composition.
26951372	2	51	theme	stress	277:282	arg1	signalling					284:293	stress signalling	277:293	stress signalling	277:293	ABA triggers seed maturation, storage activity, and stress signalling and tolerance.
26951372	6	52	theme	precocious	942:951	arg1	maturation					953:962	precocious maturation	942:962	precocious maturation	942:962	Thus, enhanced ABA during transition phases induces precocious maturation but negatively interferes with growth and development.
26951372	5	53	theme	metabolism	815:824	arg1	up-regulation					780:792	transcriptional up-regulation	764:792	transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction	764:887	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	9	54	theme	ABA	1322:1324	arg1	immunomodulation					1326:1341	ABA immunomodulation	1322:1341	ABA immunomodulation	1322:1341	Conclusively, ABA immunomodulation results in precocious ABA accumulation that generates an integrated response of stress and maturation.
26951372	8	55	theme	salt-inducible	1283:1296	arg1	proteins					1298:1305	salt-inducible proteins	1283:1305	salt-inducible proteins	1283:1305	Most of these genes are ABA- and/or stress-inducible, including alcohol and aldehyde dehydrogenases, peroxidases, chaperones, glutathione-S-transferase, drought- and salt-inducible proteins.
26951372	12	56	theme	ABA	1775:1777	arg1	signalling					1779:1788	ABA signalling	1775:1788	ABA signalling	1775:1788	This indicates that anti-ABA caryopses induce specific mechanisms to desensitize ABA signalling efficiently, which finally yields mature grains with nearly unchanged dry weight and composition.
26951372	9	57	theme	precocious	1354:1363	arg1	accumulation					1369:1380	precocious ABA accumulation	1354:1380	precocious ABA accumulation that generates an integrated response of stress and maturation	1354:1443	Conclusively, ABA immunomodulation results in precocious ABA accumulation that generates an integrated response of stress and maturation.
26951372	1	58	theme	seed	205:208	arg1	phase					218:222	the seed filling phase	201:222	the seed filling phase	201:222	Abscisic acid (ABA) accumulates in seeds during the transition to the seed filling phase.
26951372	12	59	theme	specific	1740:1747	arg1	mechanisms					1749:1758	specific mechanisms	1740:1758	specific mechanisms	1740:1758	This indicates that anti-ABA caryopses induce specific mechanisms to desensitize ABA signalling efficiently, which finally yields mature grains with nearly unchanged dry weight and composition.
26951372	13	60	theme	enormous	1921:1928	arg1	flexibilities					1958:1970	the enormous physiological and metabolic flexibilities	1917:1970	the enormous physiological and metabolic flexibilities of barley grains	1917:1987	Such compensation implicates the enormous physiological and metabolic flexibilities of barley grains to adjust effects of unnaturally high ABA amounts in order to ensure and maintain proper grain development.
26951372	3	61	from	status	353:358	arg1	grains					370:375	barley grains	363:375	barley grains	363:375	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	11	62	theme	anti-ABA	1635:1642	arg1	plants					1644:1649	anti-ABA plants	1635:1649	anti-ABA plants	1635:1649	Finally, mature grain weight and composition are unchanged in anti-ABA plants, although germination is somewhat delayed.
26951372	5	63	theme	anti-ABA	696:703	arg1	grains					705:710	anti-ABA grains	696:710	anti-ABA grains	696:710	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	2	64	theme	storage	255:261	arg1	activity					263:270	storage activity	255:270	storage activity	255:270	ABA triggers seed maturation, storage activity, and stress signalling and tolerance.
26951372	9	65	theme	integrated	1400:1409	arg1	response					1411:1418	an integrated response	1397:1418	an integrated response of stress and maturation	1397:1443	Conclusively, ABA immunomodulation results in precocious ABA accumulation that generates an integrated response of stress and maturation.
26951372	13	66	theme	proper	2071:2076	arg1	development					2084:2094	proper grain development	2071:2094	proper grain development	2071:2094	Such compensation implicates the enormous physiological and metabolic flexibilities of barley grains to adjust effects of unnaturally high ABA amounts in order to ensure and maintain proper grain development.
26951372	5	67	theme	sucrose-to-starch	797:813	arg1	metabolism					815:824	sucrose-to-starch metabolism	797:824	sucrose-to-starch metabolism	797:824	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	12	68	theme	unchanged	1850:1858	arg1	weight					1864:1869	nearly unchanged dry weight	1843:1869	nearly unchanged dry weight	1843:1869	This indicates that anti-ABA caryopses induce specific mechanisms to desensitize ABA signalling efficiently, which finally yields mature grains with nearly unchanged dry weight and composition.
26951372	7	69	theme	constitutive	1049:1060	arg1	induction					1067:1075	broad constitutive gene induction	1043:1075	broad constitutive gene induction related to biotic and abiotic stresses	1043:1114	Anti-ABA grains display broad constitutive gene induction related to biotic and abiotic stresses.
26951372	11	70	theme	mature	1582:1587	arg1	weight					1595:1600	mature grain weight	1582:1600	mature grain weight	1582:1600	Finally, mature grain weight and composition are unchanged in anti-ABA plants, although germination is somewhat delayed.
26951372	12	71	theme	mature	1824:1829	arg1	grains					1831:1836	mature grains	1824:1836	mature grains with nearly unchanged dry weight and composition	1824:1885	This indicates that anti-ABA caryopses induce specific mechanisms to desensitize ABA signalling efficiently, which finally yields mature grains with nearly unchanged dry weight and composition.
26951372	0	72	from	immunomodulation	35:50	arg1	grains					62:67	barley grains	55:67	barley grains	55:67	Increasing abscisic acid levels by immunomodulation in barley grains induces precocious maturation without changing grain composition.
26951372	0	73	theme	abscisic	11:18	arg1	levels					25:30	abscisic acid levels	11:30	abscisic acid levels	11:30	Increasing abscisic acid levels by immunomodulation in barley grains induces precocious maturation without changing grain composition.
26951372	7	74	theme	Anti-ABA	1019:1026	arg1	grains					1028:1033	Anti-ABA grains	1019:1033	Anti-ABA grains	1019:1033	Anti-ABA grains display broad constitutive gene induction related to biotic and abiotic stresses.
26951372	11	75	from	unchanged	1622:1630	arg1	plants					1644:1649	anti-ABA plants	1635:1649	anti-ABA plants	1635:1649	Finally, mature grain weight and composition are unchanged in anti-ABA plants, although germination is somewhat delayed.
26951372	4	76	theme	large	560:564	arg1	excess					566:571	large excess	560:571	large excess of free ABA	560:583	Calculation of free versus antibody-bound ABA reveals large excess of free ABA, increasing signficantly in caryopses from 10 days after fertilization.
26951372	13	77	theme	ABA	2027:2029	arg1	amounts					2031:2037	unnaturally high ABA amounts	2010:2037	unnaturally high ABA amounts	2010:2037	Such compensation implicates the enormous physiological and metabolic flexibilities of barley grains to adjust effects of unnaturally high ABA amounts in order to ensure and maintain proper grain development.
26951372	5	78	theme	signal	869:874	arg1	transduction					876:887	ABA-related signal transduction	857:887	ABA-related signal transduction	857:887	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	3	79	theme	barley	363:368	arg1	grains					370:375	barley grains	363:375	barley grains	363:375	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	8	80	theme	alcohol	1181:1187	arg1	dehydrogenases					1202:1215	alcohol and aldehyde dehydrogenases	1181:1215	dehydrogenases	1202:1215	Most of these genes are ABA- and/or stress-inducible, including alcohol and aldehyde dehydrogenases, peroxidases, chaperones, glutathione-S-transferase, drought- and salt-inducible proteins.
26951372	6	81	theme	transition	916:925	arg1	phases					927:932	transition phases	916:932	transition phases	916:932	Thus, enhanced ABA during transition phases induces precocious maturation but negatively interferes with growth and development.
26951372	3	82	theme	transgenic	397:406	arg1	caryopses					408:416	the resulting transgenic caryopses	383:416	the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA	383:503	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	0	83	theme	grain	116:120	arg1	composition					122:132	grain composition	116:132	grain composition	116:132	Increasing abscisic acid levels by immunomodulation in barley grains induces precocious maturation without changing grain composition.
26951372	0	84	theme	barley	55:60	arg1	grains					62:67	barley grains	55:67	barley grains	55:67	Increasing abscisic acid levels by immunomodulation in barley grains induces precocious maturation without changing grain composition.
26951372	8	85	theme	aldehyde	1193:1200	arg1	dehydrogenases					1202:1215	alcohol and aldehyde dehydrogenases	1181:1215	dehydrogenases	1202:1215	Most of these genes are ABA- and/or stress-inducible, including alcohol and aldehyde dehydrogenases, peroxidases, chaperones, glutathione-S-transferase, drought- and salt-inducible proteins.
26951372	12	86	with	grains	1831:1836	arg1	weight					1864:1869	nearly unchanged dry weight	1843:1869	nearly unchanged dry weight	1843:1869	This indicates that anti-ABA caryopses induce specific mechanisms to desensitize ABA signalling efficiently, which finally yields mature grains with nearly unchanged dry weight and composition.
26951372	12	86	with	grains	1831:1836	arg1	composition					1875:1885	composition	1875:1885	composition	1875:1885	This indicates that anti-ABA caryopses induce specific mechanisms to desensitize ABA signalling efficiently, which finally yields mature grains with nearly unchanged dry weight and composition.
26951372	4	87	theme	ABA	548:550	arg1	Calculation					506:516	Calculation	506:516	Calculation of free versus antibody-bound ABA	506:550	Calculation of free versus antibody-bound ABA reveals large excess of free ABA, increasing signficantly in caryopses from 10 days after fertilization.
26951372	10	88	theme	signalling	1464:1473	arg1	Repression					1446:1455	Repression	1446:1455	Repression	1446:1455	Repression of ABA signalling, occurring in anti-ABA grains, potentially antagonizes effects caused by overshooting production.
26951372	5	89	from	Metabolite	657:666	arg1	grains					705:710	anti-ABA grains	696:710	anti-ABA grains	696:710	Metabolite and transcript profiling in anti-ABA grains expose triggered and enhanced ABA-functions such as transcriptional up-regulation of sucrose-to-starch metabolism, storage protein synthesis and ABA-related signal transduction.
26951372	3	90	theme	increased	475:483	arg1	accumulation					485:496	increased accumulation	475:496	increased accumulation of ABA	475:503	Immunomodulation was used to alter the ABA status in barley grains, with the resulting transgenic caryopses responding to the anti-ABA antibody gene expression with increased accumulation of ABA.
26951372	10	91	theme	anti-ABA	1489:1496	arg1	grains					1498:1503	anti-ABA grains	1489:1503	anti-ABA grains	1489:1503	Repression of ABA signalling, occurring in anti-ABA grains, potentially antagonizes effects caused by overshooting production.
25069744	0	0	theme	high	96:99	arg1	chromatography					122:135	high performance affinity chromatography	96:135	high performance affinity chromatography	96:135	Multianalyte determination of the kinetic rate constants of drug-cyclodextrin supermolecules by high performance affinity chromatography.
25069744	3	1	theme	phase	717:721	arg1	composition					723:733	the mobile phase composition	706:733	the mobile phase composition	706:733	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	4	2	theme	extra	857:861	arg1	solvents					871:878	extra organic solvents	857:878	extra organic solvents	857:878	As a result, the kd,app values increased when decreasing the ion strength, increasing the ionization of drugs and adding extra organic solvents.
25069744	2	3	dep	constant	326:333	arg1	kd					336:337	kd	336:337	kd	336:337	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	2	3	dep	constant	326:333	arg1	app					339:341	app	339:341	app	339:341	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	6	4	theme	single	1313:1318	arg1	analysis					1329:1336	the single compound analysis	1309:1336	the single compound analysis for all of the three drugs (p>0.05)	1309:1372	For both of the single and multiple flow rate peak profiling methods, the results of the multianalyte approach were statistically equivalent with that of the single compound analysis for all of the three drugs (p>0.05).
25069744	2	5	theme	affinity	367:374	arg1	chromatography					376:389	high performance affinity chromatography	350:389	high performance affinity chromatography (HPAC)	350:396	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	2	5	theme	affinity	367:374	arg1	HPAC					392:395	HPAC	392:395	HPAC	392:395	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	0	6	theme	affinity	113:120	arg1	chromatography					122:135	high performance affinity chromatography	96:135	high performance affinity chromatography	96:135	Multianalyte determination of the kinetic rate constants of drug-cyclodextrin supermolecules by high performance affinity chromatography.
25069744	2	7	theme	constant	326:333	arg1	determination					278:290	The individual determination	263:290	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC)	263:396	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	2	7	theme	constant	326:333	arg1	process					411:417	a tedious process	401:417	a tedious process requiring numerous separate studies and massive data fitting	401:478	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	7	8	theme	less	1485:1488	arg1	variance					1490:1497	less variance	1485:1497	less variance caused by cyclodextrin column bleeding	1485:1536	The multianalyte approach can be employed for the efficient evaluation of the drug-cyclodextrin kinetics with less variance caused by cyclodextrin column bleeding.
25069744	0	9	theme	performance	101:111	arg1	chromatography					122:135	high performance affinity chromatography	96:135	high performance affinity chromatography	96:135	Multianalyte determination of the kinetic rate constants of drug-cyclodextrin supermolecules by high performance affinity chromatography.
25069744	7	10	theme	multianalyte	1379:1390	arg1	approach					1392:1399	The multianalyte approach	1375:1399	The multianalyte approach	1375:1399	The multianalyte approach can be employed for the efficient evaluation of the drug-cyclodextrin kinetics with less variance caused by cyclodextrin column bleeding.
25069744	4	11	theme	organic	863:869	arg1	solvents					871:878	extra organic solvents	857:878	extra organic solvents	857:878	As a result, the kd,app values increased when decreasing the ion strength, increasing the ionization of drugs and adding extra organic solvents.
25069744	2	12	theme	rate	321:324	arg1	constant					326:333	the apparent dissociation rate constant	295:333	the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC)	295:396	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	5	13	theme	kd	895:896	arg1	values					885:890	The values	881:890	The values of kd,app for acetaminophen, phenacetin and S-flurbiprofen estimated by the multianalyte approach	881:988	The values of kd,app for acetaminophen, phenacetin and S-flurbiprofen estimated by the multianalyte approach were 8.54±1.81, 5.36±0.94 and 0.17±0.02s(-1), respectively, which were in good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1)).
25069744	5	13	theme	kd	895:896	arg1	8.54±1.81					995:1003	8.54±1.81	995:1003	8.54±1.81	995:1003	The values of kd,app for acetaminophen, phenacetin and S-flurbiprofen estimated by the multianalyte approach were 8.54±1.81, 5.36±0.94 and 0.17±0.02s(-1), respectively, which were in good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1)).
25069744	6	14	theme	peak	1201:1204	arg1	rate					1196:1199	the single and multiple flow rate peak	1167:1204	the single and multiple flow rate peak profiling methods	1167:1222	For both of the single and multiple flow rate peak profiling methods, the results of the multianalyte approach were statistically equivalent with that of the single compound analysis for all of the three drugs (p>0.05).
25069744	5	15	dep	8.54±1.81	995:1003	arg1	agreement					1069:1077	good agreement	1064:1077	good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1))	1064:1152	The values of kd,app for acetaminophen, phenacetin and S-flurbiprofen estimated by the multianalyte approach were 8.54±1.81, 5.36±0.94 and 0.17±0.02s(-1), respectively, which were in good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1)).
25069744	2	16	theme	dissociation	308:319	arg1	constant					326:333	the apparent dissociation rate constant	295:333	the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC)	295:396	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	2	17	theme	tedious	403:409	arg1	determination					278:290	The individual determination	263:290	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC)	263:396	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	2	17	theme	tedious	403:409	arg1	process					411:417	a tedious process	401:417	a tedious process requiring numerous separate studies and massive data fitting	401:478	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	3	18	from	dependence	650:659	arg1	composition					723:733	the mobile phase composition	706:733	the mobile phase composition	706:733	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	2	19	theme	massive	459:465	arg1	fitting					472:478	massive data fitting	459:478	massive data fitting	459:478	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	6	20	with	equivalent	1285:1294	arg1	that					1301:1304	that	1301:1304	that	1301:1304	For both of the single and multiple flow rate peak profiling methods, the results of the multianalyte approach were statistically equivalent with that of the single compound analysis for all of the three drugs (p>0.05).
25069744	2	21	theme	data	467:470	arg1	fitting					472:478	massive data fitting	459:478	massive data fitting	459:478	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	5	22	dep	determined	1090:1099	arg1	5.01±0.42					1124:1132	5.01±0.42	1124:1132	5.01±0.42	1124:1132	The values of kd,app for acetaminophen, phenacetin and S-flurbiprofen estimated by the multianalyte approach were 8.54±1.81, 5.36±0.94 and 0.17±0.02s(-1), respectively, which were in good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1)).
25069744	5	22	dep	determined	1090:1099	arg1	8.31±0.58					1113:1121	8.31±0.58	1113:1121	8.31±0.58	1113:1121	The values of kd,app for acetaminophen, phenacetin and S-flurbiprofen estimated by the multianalyte approach were 8.54±1.81, 5.36±0.94 and 0.17±0.02s(-1), respectively, which were in good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1)).
25069744	5	22	dep	determined	1090:1099	arg1	-1					1149:1150	-1	1149:1150	-1	1149:1150	The values of kd,app for acetaminophen, phenacetin and S-flurbiprofen estimated by the multianalyte approach were 8.54±1.81, 5.36±0.94 and 0.17±0.02s(-1), respectively, which were in good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1)).
25069744	5	22	dep	determined	1090:1099	arg1	0.15±0.01s					1138:1147	0.15±0.01s	1138:1147	0.15±0.01s	1138:1147	The values of kd,app for acetaminophen, phenacetin and S-flurbiprofen estimated by the multianalyte approach were 8.54±1.81, 5.36±0.94 and 0.17±0.02s(-1), respectively, which were in good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1)).
25069744	6	23	theme	flow	1191:1194	arg1	rate					1196:1199	the single and multiple flow rate peak	1167:1204	the single and multiple flow rate peak profiling methods	1167:1222	For both of the single and multiple flow rate peak profiling methods, the results of the multianalyte approach were statistically equivalent with that of the single compound analysis for all of the three drugs (p>0.05).
25069744	6	24	theme	multiple	1182:1189	arg1	rate					1196:1199	the single and multiple flow rate peak	1167:1204	the single and multiple flow rate peak profiling methods	1167:1222	For both of the single and multiple flow rate peak profiling methods, the results of the multianalyte approach were statistically equivalent with that of the single compound analysis for all of the three drugs (p>0.05).
25069744	5	25	theme	good	1064:1067	arg1	agreement					1069:1077	good agreement	1064:1077	good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1))	1064:1152	The values of kd,app for acetaminophen, phenacetin and S-flurbiprofen estimated by the multianalyte approach were 8.54±1.81, 5.36±0.94 and 0.17±0.02s(-1), respectively, which were in good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1)).
25069744	0	26	theme	Multianalyte	0:11	arg1	determination					13:25	Multianalyte determination	0:25	Multianalyte determination of the kinetic rate constants of drug-cyclodextrin	0:76	Multianalyte determination of the kinetic rate constants of drug-cyclodextrin supermolecules by high performance affinity chromatography.
25069744	4	27	theme	drugs	840:844	arg1	ionization					826:835	the ionization	822:835	the ionization of drugs	822:844	As a result, the kd,app values increased when decreasing the ion strength, increasing the ionization of drugs and adding extra organic solvents.
25069744	4	28	theme	ion	797:799	arg1	strength					801:808	the ion strength	793:808	the ion strength	793:808	As a result, the kd,app values increased when decreasing the ion strength, increasing the ionization of drugs and adding extra organic solvents.
25069744	3	29	theme	drugs	588:592	arg1	kd					565:566	the kd	561:566	the kd	561:566	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	3	29	theme	drugs	588:592	arg1	values					572:577	app values	568:577	app values of three drugs	568:592	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	1	30	theme	in	211:212	arg1	performance					219:229	the in vivo performance	207:229	the in vivo performance	207:229	The kinetics of the dissociation is fundamental to the formation and the in vivo performance of cyclodextrin supramolecules.
25069744	7	31	theme	kinetics	1471:1478	arg1	evaluation					1435:1444	the efficient evaluation	1421:1444	the efficient evaluation of the drug-cyclodextrin kinetics with less variance caused by cyclodextrin column bleeding	1421:1536	The multianalyte approach can be employed for the efficient evaluation of the drug-cyclodextrin kinetics with less variance caused by cyclodextrin column bleeding.
25069744	3	32	theme	app	568:570	arg1	kd					565:566	the kd	561:566	the kd	561:566	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	3	32	theme	app	568:570	arg1	values					572:577	app values	568:577	app values of three drugs	568:592	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	7	33	with	kinetics	1471:1478	arg1	variance					1490:1497	less variance	1485:1497	less variance caused by cyclodextrin column bleeding	1485:1536	The multianalyte approach can be employed for the efficient evaluation of the drug-cyclodextrin kinetics with less variance caused by cyclodextrin column bleeding.
25069744	2	34	theme	numerous	429:436	arg1	studies					447:453	numerous separate studies	429:453	numerous separate studies	429:453	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	0	35	theme	rate	42:45	arg1	constants					47:55	the kinetic rate constants	30:55	the kinetic rate constants of drug-cyclodextrin	30:76	Multianalyte determination of the kinetic rate constants of drug-cyclodextrin supermolecules by high performance affinity chromatography.
25069744	3	36	theme	multianalyte	500:511	arg1	approach					513:520	the multianalyte approach	496:520	the multianalyte approach	496:520	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	3	37	theme	dependence	650:659	arg1	investigation					629:641	the investigation	625:641	the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition	625:733	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	0	38	theme	kinetic	34:40	arg1	constants					47:55	the kinetic rate constants	30:55	the kinetic rate constants of drug-cyclodextrin	30:76	Multianalyte determination of the kinetic rate constants of drug-cyclodextrin supermolecules by high performance affinity chromatography.
25069744	6	39	theme	approach	1257:1264	arg1	results					1229:1235	the results	1225:1235	the results of the multianalyte approach	1225:1264	For both of the single and multiple flow rate peak profiling methods, the results of the multianalyte approach were statistically equivalent with that of the single compound analysis for all of the three drugs (p>0.05).
25069744	6	39	theme	approach	1257:1264	arg1	equivalent					1285:1294	equivalent	1285:1294	equivalent	1285:1294	For both of the single and multiple flow rate peak profiling methods, the results of the multianalyte approach were statistically equivalent with that of the single compound analysis for all of the three drugs (p>0.05).
25069744	6	40	theme	single	1171:1176	arg1	rate					1196:1199	the single and multiple flow rate peak	1167:1204	the single and multiple flow rate peak profiling methods	1167:1222	For both of the single and multiple flow rate peak profiling methods, the results of the multianalyte approach were statistically equivalent with that of the single compound analysis for all of the three drugs (p>0.05).
25069744	3	41	from	investigation	629:641	arg1	composition					723:733	the mobile phase composition	706:733	the mobile phase composition	706:733	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	6	42	theme	multianalyte	1244:1255	arg1	approach					1257:1264	the multianalyte approach	1240:1264	the multianalyte approach	1240:1264	For both of the single and multiple flow rate peak profiling methods, the results of the multianalyte approach were statistically equivalent with that of the single compound analysis for all of the three drugs (p>0.05).
25069744	7	43	theme	drug-cyclodextrin	1453:1469	arg1	kinetics					1471:1478	the drug-cyclodextrin kinetics	1449:1478	the drug-cyclodextrin kinetics with less variance caused by cyclodextrin column bleeding	1449:1536	The multianalyte approach can be employed for the efficient evaluation of the drug-cyclodextrin kinetics with less variance caused by cyclodextrin column bleeding.
25069744	2	44	theme	performance	355:365	arg1	chromatography					376:389	high performance affinity chromatography	350:389	high performance affinity chromatography (HPAC)	350:396	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	2	44	theme	performance	355:365	arg1	HPAC					392:395	HPAC	392:395	HPAC	392:395	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	0	45	theme	constants	47:55	arg1	determination					13:25	Multianalyte determination	0:25	Multianalyte determination of the kinetic rate constants of drug-cyclodextrin	0:76	Multianalyte determination of the kinetic rate constants of drug-cyclodextrin supermolecules by high performance affinity chromatography.
25069744	3	46	theme	interaction	682:692	arg1	kinetics					694:701	drug-cyclodextrin interaction kinetics	664:701	drug-cyclodextrin interaction kinetics	664:701	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	1	47	dep	formation	193:201	arg1	the					189:191	the	189:191	the	189:191	The kinetics of the dissociation is fundamental to the formation and the in vivo performance of cyclodextrin supramolecules.
25069744	2	48	theme	high	350:353	arg1	chromatography					376:389	high performance affinity chromatography	350:389	high performance affinity chromatography (HPAC)	350:396	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	2	48	theme	high	350:353	arg1	HPAC					392:395	HPAC	392:395	HPAC	392:395	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	5	49	with	agreement	1069:1077	arg1	those					1084:1088	those	1084:1088	those	1084:1088	The values of kd,app for acetaminophen, phenacetin and S-flurbiprofen estimated by the multianalyte approach were 8.54±1.81, 5.36±0.94 and 0.17±0.02s(-1), respectively, which were in good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1)).
25069744	6	50	theme	compound	1320:1327	arg1	analysis					1329:1336	the single compound analysis	1309:1336	the single compound analysis for all of the three drugs (p>0.05)	1309:1372	For both of the single and multiple flow rate peak profiling methods, the results of the multianalyte approach were statistically equivalent with that of the single compound analysis for all of the three drugs (p>0.05).
25069744	3	51	theme	kinetics	694:701	arg1	dependence					650:659	the dependence	646:659	the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition	646:733	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	5	52	theme	multianalyte	968:979	arg1	approach					981:988	the multianalyte approach	964:988	the multianalyte approach	964:988	The values of kd,app for acetaminophen, phenacetin and S-flurbiprofen estimated by the multianalyte approach were 8.54±1.81, 5.36±0.94 and 0.17±0.02s(-1), respectively, which were in good agreement with those determined separately (8.31±0.58, 5.01±0.42 and 0.15±0.01s(-1)).
25069744	1	53	theme	cyclodextrin	234:245	arg1	supramolecules					247:260	cyclodextrin supramolecules	234:260	cyclodextrin supramolecules	234:260	The kinetics of the dissociation is fundamental to the formation and the in vivo performance of cyclodextrin supramolecules.
25069744	2	54	theme	apparent	299:306	arg1	constant					326:333	the apparent dissociation rate constant	295:333	the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC)	295:396	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	4	55	theme	app	756:758	arg1	values					760:765	app values	756:765	app values	756:765	As a result, the kd,app values increased when decreasing the ion strength, increasing the ionization of drugs and adding extra organic solvents.
25069744	1	56	theme	supramolecules	247:260	arg1	performance					219:229	the in vivo performance	207:229	the in vivo performance	207:229	The kinetics of the dissociation is fundamental to the formation and the in vivo performance of cyclodextrin supramolecules.
25069744	1	56	theme	supramolecules	247:260	arg1	formation					193:201	formation	193:201	formation	193:201	The kinetics of the dissociation is fundamental to the formation and the in vivo performance of cyclodextrin supramolecules.
25069744	3	57	theme	drug-cyclodextrin	664:680	arg1	kinetics					694:701	drug-cyclodextrin interaction kinetics	664:701	drug-cyclodextrin interaction kinetics	664:701	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	1	58	dep	in	211:212	arg1	vivo					214:217	vivo	214:217	vivo	214:217	The kinetics of the dissociation is fundamental to the formation and the in vivo performance of cyclodextrin supramolecules.
25069744	2	59	theme	separate	438:445	arg1	studies					447:453	numerous separate studies	429:453	numerous separate studies	429:453	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	1	60	theme	dissociation	158:169	arg1	fundamental					174:184	fundamental	174:184	fundamental	174:184	The kinetics of the dissociation is fundamental to the formation and the in vivo performance of cyclodextrin supramolecules.
25069744	1	60	theme	dissociation	158:169	arg1	kinetics					142:149	The kinetics	138:149	The kinetics of the dissociation	138:169	The kinetics of the dissociation is fundamental to the formation and the in vivo performance of cyclodextrin supramolecules.
25069744	0	61	theme	drug-cyclodextrin	60:76	arg1	constants					47:55	the kinetic rate constants	30:55	the kinetic rate constants of drug-cyclodextrin	30:76	Multianalyte determination of the kinetic rate constants of drug-cyclodextrin supermolecules by high performance affinity chromatography.
25069744	3	62	theme	mobile	710:715	arg1	composition					723:733	the mobile phase composition	706:733	the mobile phase composition	706:733	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	2	63	theme	individual	267:276	arg1	determination					278:290	The individual determination	263:290	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC)	263:396	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	2	63	theme	individual	267:276	arg1	process					411:417	a tedious process	401:417	a tedious process requiring numerous separate studies and massive data fitting	401:478	The individual determination of the apparent dissociation rate constant (kd,app) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate studies and massive data fitting.
25069744	3	64	from	composition	723:733	arg1	investigation					629:641	the investigation	625:641	the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition	625:733	In this study, the multianalyte approach was employed to simultaneously measure the kd,app values of three drugs through one injection based on the investigation of the dependence of drug-cyclodextrin interaction kinetics on the mobile phase composition.
25069744	7	65	theme	efficient	1425:1433	arg1	evaluation					1435:1444	the efficient evaluation	1421:1444	the efficient evaluation of the drug-cyclodextrin kinetics with less variance caused by cyclodextrin column bleeding	1421:1536	The multianalyte approach can be employed for the efficient evaluation of the drug-cyclodextrin kinetics with less variance caused by cyclodextrin column bleeding.
25563939	2	0	theme	fibres	483:488	arg1	isolation					460:468	the isolation	456:468	the isolation of cellulose fibres	456:488	This thermo-chemico-mechanical extraction leads to the isolation of cellulose fibres as observed by SEM images.
25563939	7	1	theme	steam	978:982	arg1	explosion					984:992	dilute-acid steam explosion	966:992	dilute-acid steam explosion	966:992	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	7	1	theme	steam	978:982	arg1	process					1042:1048	a valuable cellulose extraction process	1010:1048	a valuable cellulose extraction process that can provide a variety of fibre structures	1010:1095	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	1	2	theme	conditions	249:258	arg1	effect					228:233	the effect	224:233	the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites	224:402	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	2	3	theme	cellulose	473:481	arg1	fibres					483:488	cellulose fibres	473:488	cellulose fibres	473:488	This thermo-chemico-mechanical extraction leads to the isolation of cellulose fibres as observed by SEM images.
25563939	6	4	theme	severity	894:901	arg1	threshold					903:911	a severity threshold	892:911	a severity threshold	892:911	Above a severity threshold, a structural disorganization was observed.
25563939	4	5	theme	severity	676:683	arg1	effect					719:724	a tangible effect	708:724	a tangible effect of physical degradation	708:748	Likewise, fibre whiteness diminished with an increasing severity factor, which could be a tangible effect of physical degradation.
25563939	4	5	theme	severity	676:683	arg1	factor					685:690	an increasing severity factor	662:690	an increasing severity factor	662:690	Likewise, fibre whiteness diminished with an increasing severity factor, which could be a tangible effect of physical degradation.
25563939	1	6	theme	cellulose	323:331	arg1	structures					339:348	cellulose fibre structures	323:348	cellulose fibre structures in a perspective of using these in polymer composites	323:402	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	4	7	theme	degradation	738:748	arg1	effect					719:724	a tangible effect	708:724	a tangible effect of physical degradation	708:748	Likewise, fibre whiteness diminished with an increasing severity factor, which could be a tangible effect of physical degradation.
25563939	4	7	theme	degradation	738:748	arg1	factor					685:690	an increasing severity factor	662:690	an increasing severity factor	662:690	Likewise, fibre whiteness diminished with an increasing severity factor, which could be a tangible effect of physical degradation.
25563939	1	8	theme	fibre	333:337	arg1	structures					339:348	cellulose fibre structures	323:348	cellulose fibre structures in a perspective of using these in polymer composites	323:402	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	4	9	theme	physical	729:736	arg1	degradation					738:748	physical degradation	729:748	physical degradation	729:748	Likewise, fibre whiteness diminished with an increasing severity factor, which could be a tangible effect of physical degradation.
25563939	0	10	theme	temperature	110:120	arg1	impact					91:96	impact	91:96	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.	0:140	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	7	11	theme	fibre	1080:1084	arg1	structures					1086:1095	fibre structures	1080:1095	fibre structures	1080:1095	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	3	12	theme	Fibre	517:521	arg1	length					523:528	Fibre length	517:528	Fibre length as well as length to diameter aspect ratios	517:572	Fibre length as well as length to diameter aspect ratios decreased with the severity of the treatment.
25563939	0	13	theme	reaction	101:108	arg1	temperature					110:120	reaction temperature	101:120	reaction temperature	101:120	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	7	14	theme	structures	1086:1095	arg1	variety					1069:1075	a variety	1067:1075	a variety of fibre structures	1067:1095	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	7	14	theme	structures	1086:1095	arg1	structures					1086:1095	fibre structures	1080:1095	fibre structures	1080:1095	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	1	15	theme	miyabeana	179:187	arg1	explosion					160:168	Dilute-acid steam explosion	142:168	Dilute-acid steam explosion of Salix miyabeana	142:187	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	4	16	theme	tangible	710:717	arg1	effect					719:724	a tangible effect	708:724	a tangible effect of physical degradation	708:748	Likewise, fibre whiteness diminished with an increasing severity factor, which could be a tangible effect of physical degradation.
25563939	4	16	theme	tangible	710:717	arg1	factor					685:690	an increasing severity factor	662:690	an increasing severity factor	662:690	Likewise, fibre whiteness diminished with an increasing severity factor, which could be a tangible effect of physical degradation.
25563939	0	17	theme	Structural	0:9	arg1	changes					11:17	Structural changes	0:17	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.	0:140	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	3	18	theme	diameter	551:558	arg1	ratios					567:572	diameter aspect ratios	551:572	diameter aspect ratios	551:572	Fibre length as well as length to diameter aspect ratios decreased with the severity of the treatment.
25563939	0	19	theme	fibres	48:53	arg1	changes					11:17	Structural changes	0:17	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.	0:140	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	0	20	theme	Salix	22:26	arg1	fibres					48:53	Salix miyabeana cellulose fibres	22:53	Salix miyabeana cellulose fibres	22:53	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	1	21	theme	severity	281:288	arg1	factors					290:296	a severity factors	279:296	a severity factors (SFT)	279:302	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	1	21	theme	severity	281:288	arg1	SFT					299:301	SFT	299:301	SFT	299:301	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	0	22	theme	retention	126:134	arg1	time					136:139	retention time	126:139	retention time	126:139	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	5	23	theme	reaction	811:818	arg1	temperature					820:830	the reaction temperature	807:830	the reaction temperature	807:830	Variations in crystallinity seemed to be dependent upon the reaction temperature, generally decreasing with regards to retention time.
25563939	1	24	from	structures	339:348	arg1	perspective					355:365	a perspective	353:365	a perspective of using these in polymer composites	353:402	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	7	25	theme	cellulose	1021:1029	arg1	explosion					984:992	dilute-acid steam explosion	966:992	dilute-acid steam explosion	966:992	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	7	25	theme	cellulose	1021:1029	arg1	process					1042:1048	a valuable cellulose extraction process	1010:1048	a valuable cellulose extraction process that can provide a variety of fibre structures	1010:1095	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	4	26	theme	increasing	665:674	arg1	effect					719:724	a tangible effect	708:724	a tangible effect of physical degradation	708:748	Likewise, fibre whiteness diminished with an increasing severity factor, which could be a tangible effect of physical degradation.
25563939	4	26	theme	increasing	665:674	arg1	factor					685:690	an increasing severity factor	662:690	an increasing severity factor	662:690	Likewise, fibre whiteness diminished with an increasing severity factor, which could be a tangible effect of physical degradation.
25563939	0	27	theme	cellulose	38:46	arg1	fibres					48:53	Salix miyabeana cellulose fibres	22:53	Salix miyabeana cellulose fibres	22:53	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	0	28	theme	miyabeana	28:36	arg1	fibres					48:53	Salix miyabeana cellulose fibres	22:53	Salix miyabeana cellulose fibres	22:53	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	0	29	theme	time	136:139	arg1	impact					91:96	impact	91:96	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.	0:140	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	3	30	theme	treatment	609:617	arg1	severity					593:600	the severity	589:600	the severity of the treatment	589:617	Fibre length as well as length to diameter aspect ratios decreased with the severity of the treatment.
25563939	2	31	theme	thermo-chemico-mechanical	410:434	arg1	extraction					436:445	This thermo-chemico-mechanical extraction	405:445	This thermo-chemico-mechanical extraction	405:445	This thermo-chemico-mechanical extraction leads to the isolation of cellulose fibres as observed by SEM images.
25563939	2	32	theme	SEM	505:507	arg1	images					509:514	SEM images	505:514	SEM images	505:514	This thermo-chemico-mechanical extraction leads to the isolation of cellulose fibres as observed by SEM images.
25563939	5	33	theme	retention	870:878	arg1	time					880:883	retention time	870:883	retention time	870:883	Variations in crystallinity seemed to be dependent upon the reaction temperature, generally decreasing with regards to retention time.
25563939	7	34	theme	extraction	1031:1040	arg1	explosion					984:992	dilute-acid steam explosion	966:992	dilute-acid steam explosion	966:992	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	7	34	theme	extraction	1031:1040	arg1	process					1042:1048	a valuable cellulose extraction process	1010:1048	a valuable cellulose extraction process that can provide a variety of fibre structures	1010:1095	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	3	35	theme	aspect	560:565	arg1	ratios					567:572	diameter aspect ratios	551:572	diameter aspect ratios	551:572	Fibre length as well as length to diameter aspect ratios decreased with the severity of the treatment.
25563939	1	36	from	changes	312:318	arg1	structures					339:348	cellulose fibre structures	323:348	cellulose fibre structures in a perspective of using these in polymer composites	323:402	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	6	37	theme	structural	916:925	arg1	disorganization					927:941	a structural disorganization	914:941	a structural disorganization	914:941	Above a severity threshold, a structural disorganization was observed.
25563939	5	38	from	Variations	751:760	arg1	crystallinity					765:777	crystallinity	765:777	crystallinity	765:777	Variations in crystallinity seemed to be dependent upon the reaction temperature, generally decreasing with regards to retention time.
25563939	7	39	theme	valuable	1012:1019	arg1	explosion					984:992	dilute-acid steam explosion	966:992	dilute-acid steam explosion	966:992	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	7	39	theme	valuable	1012:1019	arg1	process					1042:1048	a valuable cellulose extraction process	1010:1048	a valuable cellulose extraction process that can provide a variety of fibre structures	1010:1095	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	1	40	theme	polymer	385:391	arg1	composites					393:402	polymer composites	385:402	polymer composites	385:402	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	1	41	from	effect	228:233	arg1	changes					312:318	the changes	308:318	the changes in cellulose fibre structures in a perspective of using these in polymer composites	308:402	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	0	42	theme	steam	74:78	arg1	explosion					80:88	dilute-acid steam explosion	62:88	dilute-acid steam explosion	62:88	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	0	43	dep	changes	11:17	arg1	impact					91:96	impact	91:96	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.	0:140	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	1	44	theme	Dilute-acid	142:152	arg1	explosion					160:168	Dilute-acid steam explosion	142:168	Dilute-acid steam explosion of Salix miyabeana	142:187	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	4	45	theme	fibre	630:634	arg1	whiteness					636:644	fibre whiteness	630:644	fibre whiteness	630:644	Likewise, fibre whiteness diminished with an increasing severity factor, which could be a tangible effect of physical degradation.
25563939	0	46	theme	dilute-acid	62:72	arg1	explosion					80:88	dilute-acid steam explosion	62:88	dilute-acid steam explosion	62:88	Structural changes of Salix miyabeana cellulose fibres during dilute-acid steam explosion: impact of reaction temperature and retention time.
25563939	7	47	theme	dilute-acid	966:976	arg1	explosion					984:992	dilute-acid steam explosion	966:992	dilute-acid steam explosion	966:992	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	7	47	theme	dilute-acid	966:976	arg1	process					1042:1048	a valuable cellulose extraction process	1010:1048	a valuable cellulose extraction process that can provide a variety of fibre structures	1010:1095	Overall, dilute-acid steam explosion was shown to be a valuable cellulose extraction process that can provide a variety of fibre structures.
25563939	1	48	theme	steam	154:158	arg1	explosion					160:168	Dilute-acid steam explosion	142:168	Dilute-acid steam explosion of Salix miyabeana	142:187	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
25563939	1	49	theme	processing	238:247	arg1	conditions					249:258	processing conditions	238:258	processing conditions	238:258	Dilute-acid steam explosion of Salix miyabeana has been carried out to understand the effect of processing conditions, expressed through a severity factors (SFT), on the changes in cellulose fibre structures in a perspective of using these in polymer composites.
26773010	4	0	theme	microbiota	474:483	arg1	composition					455:465	the complex composition	443:465	the complex composition of the microbiota	443:483	Given the complex composition of the microbiota, the demonstration of such beneficial effects is difficult.
26773010	7	1	theme	considerable	929:940	arg1	difference					942:951	the considerable difference	925:951	the considerable difference between recommended and actual fiber intakes by the average consumer	925:1020	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	7	1	theme	considerable	929:940	arg1	-that					915:919	the "fiber gap"-that	900:919	the "fiber gap"-that	900:919	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	5	2	theme	fermentation	635:646	arg1	result					621:626	a result	619:626	a result of its fermentation in the colon	619:659	In contrast, an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon offers better perspectives for providing mechanistic links between fiber intake and health benefits.
26773010	5	3	theme	health	745:750	arg1	benefits					752:759	health benefits	745:759	health benefits	745:759	In contrast, an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon offers better perspectives for providing mechanistic links between fiber intake and health benefits.
26773010	2	4	contain	have	217:220	arg2	effects					244:250	different physiologic effects	222:250	different physiologic effects	222:250	The collective term "dietary fiber" groups structures that have different physiologic effects.
26773010	2	4	contain	have	217:220	arg1	structures					201:210	structures	201:210	structures that have different physiologic effects	201:250	The collective term "dietary fiber" groups structures that have different physiologic effects.
26773010	7	5	theme	gap	911:913	arg1	difference					942:951	the considerable difference	925:951	the considerable difference between recommended and actual fiber intakes by the average consumer	925:1020	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	7	5	theme	gap	911:913	arg1	-that					915:919	the "fiber gap"-that	900:919	the "fiber gap"-that	900:919	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	6	6	theme	studies	788:794	arg1	outcomes					771:778	Positive outcomes	762:778	Positive outcomes of such studies	762:794	Positive outcomes of such studies hold the promise that claims describing specific health benefits can be granted.
26773010	5	7	theme	mechanistic	702:712	arg1	links					714:718	mechanistic links	702:718	mechanistic links between fiber intake and health benefits	702:759	In contrast, an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon offers better perspectives for providing mechanistic links between fiber intake and health benefits.
26773010	5	8	theme	metabolites	580:590	arg1	exploration					561:571	an exploration	558:571	an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon	558:659	In contrast, an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon offers better perspectives for providing mechanistic links between fiber intake and health benefits.
26773010	4	9	theme	complex	447:453	arg1	composition					455:465	the complex composition	443:465	the complex composition of the microbiota	443:483	Given the complex composition of the microbiota, the demonstration of such beneficial effects is difficult.
26773010	5	10	theme	dietary	595:601	arg1	fiber					603:607	dietary fiber	595:607	dietary fiber	595:607	In contrast, an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon offers better perspectives for providing mechanistic links between fiber intake and health benefits.
26773010	7	11	dep	bridge	893:898	arg1	difference					942:951	the considerable difference	925:951	the considerable difference between recommended and actual fiber intakes by the average consumer	925:1020	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	7	11	dep	bridge	893:898	arg1	-that					915:919	the "fiber gap"-that	900:919	the "fiber gap"-that	900:919	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	5	12	theme	fiber	603:607	arg1	metabolites					580:590	the metabolites	576:590	the metabolites of dietary fiber formed as a result of its fermentation in the colon	576:659	In contrast, an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon offers better perspectives for providing mechanistic links between fiber intake and health benefits.
26773010	7	13	theme	fiber	905:909	arg1	difference					942:951	the considerable difference	925:951	the considerable difference between recommended and actual fiber intakes by the average consumer	925:1020	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	7	13	theme	fiber	905:909	arg1	-that					915:919	the "fiber gap"-that	900:919	the "fiber gap"-that	900:919	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	3	14	theme	gastrointestinal	408:423	arg1	microbiota					425:434	the gastrointestinal microbiota	404:434	the gastrointestinal microbiota	404:434	Since 1995, some dietary fibers have been denoted as prebiotics, implying a beneficial physiologic effect related to increasing numbers or activity of the gastrointestinal microbiota.
26773010	6	15	theme	Positive	762:769	arg1	outcomes					771:778	Positive outcomes	762:778	Positive outcomes of such studies	762:794	Positive outcomes of such studies hold the promise that claims describing specific health benefits can be granted.
26773010	3	16	theme	related	359:365	arg1	effect					352:357	a beneficial physiologic effect	327:357	a beneficial physiologic effect related to increasing numbers or activity of the gastrointestinal microbiota	327:434	Since 1995, some dietary fibers have been denoted as prebiotics, implying a beneficial physiologic effect related to increasing numbers or activity of the gastrointestinal microbiota.
26773010	1	17	from	effects	88:94	arg1	health					116:121	health	116:121	health	116:121	Since the 1970s, the positive effects of dietary fiber on health have increasingly been recognized.
26773010	3	18	theme	beneficial	329:338	arg1	effect					352:357	a beneficial physiologic effect	327:357	a beneficial physiologic effect related to increasing numbers or activity of the gastrointestinal microbiota	327:434	Since 1995, some dietary fibers have been denoted as prebiotics, implying a beneficial physiologic effect related to increasing numbers or activity of the gastrointestinal microbiota.
26773010	3	19	theme	physiologic	340:350	arg1	effect					352:357	a beneficial physiologic effect	327:357	a beneficial physiologic effect related to increasing numbers or activity of the gastrointestinal microbiota	327:434	Since 1995, some dietary fibers have been denoted as prebiotics, implying a beneficial physiologic effect related to increasing numbers or activity of the gastrointestinal microbiota.
26773010	5	20	theme	better	668:673	arg1	perspectives					675:686	better perspectives	668:686	better perspectives for providing mechanistic links between fiber intake and health benefits	668:759	In contrast, an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon offers better perspectives for providing mechanistic links between fiber intake and health benefits.
26773010	1	21	theme	positive	79:86	arg1	effects					88:94	the positive effects	75:94	the positive effects of dietary fiber on health	75:121	Since the 1970s, the positive effects of dietary fiber on health have increasingly been recognized.
26773010	6	22	theme	such	783:786	arg1	studies					788:794	such studies	783:794	such studies	783:794	Positive outcomes of such studies hold the promise that claims describing specific health benefits can be granted.
26773010	6	23	theme	health	845:850	arg1	benefits					852:859	specific health benefits	836:859	specific health benefits	836:859	Positive outcomes of such studies hold the promise that claims describing specific health benefits can be granted.
26773010	3	24	theme	microbiota	425:434	arg1	activity					392:399	activity	392:399	activity	392:399	Since 1995, some dietary fibers have been denoted as prebiotics, implying a beneficial physiologic effect related to increasing numbers or activity of the gastrointestinal microbiota.
26773010	3	24	theme	microbiota	425:434	arg1	numbers					381:387	numbers	381:387	numbers	381:387	Since 1995, some dietary fibers have been denoted as prebiotics, implying a beneficial physiologic effect related to increasing numbers or activity of the gastrointestinal microbiota.
26773010	4	25	theme	such	507:510	arg1	effects					523:529	such beneficial effects	507:529	such beneficial effects	507:529	Given the complex composition of the microbiota, the demonstration of such beneficial effects is difficult.
26773010	6	26	theme	specific	836:843	arg1	benefits					852:859	specific health benefits	836:859	specific health benefits	836:859	Positive outcomes of such studies hold the promise that claims describing specific health benefits can be granted.
26773010	0	27	theme	Dietary	24:30	arg1	Fiber					32:36	Fermentable Dietary Fiber	12:36	Fermentable Dietary Fiber	12:36	Prebiotics, Fermentable Dietary Fiber, and Health Claims.
26773010	7	28	theme	actual	977:982	arg1	intakes					990:996	recommended and actual fiber intakes	961:996	recommended and actual fiber intakes by the average consumer	961:1020	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	5	29	from	result	621:626	arg1	colon					655:659	the colon	651:659	the colon	651:659	In contrast, an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon offers better perspectives for providing mechanistic links between fiber intake and health benefits.
26773010	0	30	theme	Health	43:48	arg1	Claims					50:55	Health Claims	43:55	Health Claims	43:55	Prebiotics, Fermentable Dietary Fiber, and Health Claims.
26773010	3	31	theme	dietary	270:276	arg1	prebiotics					306:315	prebiotics	306:315	prebiotics	306:315	Since 1995, some dietary fibers have been denoted as prebiotics, implying a beneficial physiologic effect related to increasing numbers or activity of the gastrointestinal microbiota.
26773010	3	31	theme	dietary	270:276	arg1	fibers					278:283	some dietary fibers	265:283	some dietary fibers	265:283	Since 1995, some dietary fibers have been denoted as prebiotics, implying a beneficial physiologic effect related to increasing numbers or activity of the gastrointestinal microbiota.
26773010	7	32	theme	fiber	984:988	arg1	intakes					990:996	recommended and actual fiber intakes	961:996	recommended and actual fiber intakes by the average consumer	961:1020	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	4	33	theme	effects	523:529	arg1	difficult					534:542	difficult	534:542	difficult	534:542	Given the complex composition of the microbiota, the demonstration of such beneficial effects is difficult.
26773010	4	33	theme	effects	523:529	arg1	demonstration					490:502	the demonstration	486:502	the demonstration of such beneficial effects	486:529	Given the complex composition of the microbiota, the demonstration of such beneficial effects is difficult.
26773010	7	34	theme	average	1005:1011	arg1	consumer					1013:1020	the average consumer	1001:1020	the average consumer	1001:1020	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	2	35	theme	dietary	179:185	arg1	"					192:192	The collective term "dietary fiber"	158:192	The collective term "dietary fiber"	158:192	The collective term "dietary fiber" groups structures that have different physiologic effects.
26773010	2	36	theme	fiber	187:191	arg1	"					192:192	The collective term "dietary fiber"	158:192	The collective term "dietary fiber"	158:192	The collective term "dietary fiber" groups structures that have different physiologic effects.
26773010	7	37	theme	recommended	961:971	arg1	intakes					990:996	recommended and actual fiber intakes	961:996	recommended and actual fiber intakes by the average consumer	961:1020	This would help bridge the "fiber gap"-that is, the considerable difference between recommended and actual fiber intakes by the average consumer.
26773010	4	38	theme	beneficial	512:521	arg1	effects					523:529	such beneficial effects	507:529	such beneficial effects	507:529	Given the complex composition of the microbiota, the demonstration of such beneficial effects is difficult.
26773010	2	39	theme	physiologic	232:242	arg1	effects					244:250	different physiologic effects	222:250	different physiologic effects	222:250	The collective term "dietary fiber" groups structures that have different physiologic effects.
26773010	1	40	theme	dietary	99:105	arg1	fiber					107:111	dietary fiber	99:111	dietary fiber	99:111	Since the 1970s, the positive effects of dietary fiber on health have increasingly been recognized.
26773010	2	41	theme	term	173:176	arg1	"					192:192	The collective term "dietary fiber"	158:192	The collective term "dietary fiber"	158:192	The collective term "dietary fiber" groups structures that have different physiologic effects.
26773010	2	42	theme	different	222:230	arg1	effects					244:250	different physiologic effects	222:250	different physiologic effects	222:250	The collective term "dietary fiber" groups structures that have different physiologic effects.
26773010	1	43	theme	fiber	107:111	arg1	effects					88:94	the positive effects	75:94	the positive effects of dietary fiber on health	75:121	Since the 1970s, the positive effects of dietary fiber on health have increasingly been recognized.
26773010	5	44	from	colon	655:659	arg1	result					621:626	a result	619:626	a result of its fermentation in the colon	619:659	In contrast, an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon offers better perspectives for providing mechanistic links between fiber intake and health benefits.
26773010	5	45	theme	fiber	728:732	arg1	intake					734:739	fiber intake	728:739	fiber intake	728:739	In contrast, an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon offers better perspectives for providing mechanistic links between fiber intake and health benefits.
26773010	2	46	theme	collective	162:171	arg1	"					192:192	The collective term "dietary fiber"	158:192	The collective term "dietary fiber"	158:192	The collective term "dietary fiber" groups structures that have different physiologic effects.
26773010	5	47	from	fermentation	635:646	arg1	colon					655:659	the colon	651:659	the colon	651:659	In contrast, an exploration of the metabolites of dietary fiber formed as a result of its fermentation in the colon offers better perspectives for providing mechanistic links between fiber intake and health benefits.
26773010	0	48	theme	Fermentable	12:22	arg1	Fiber					32:36	Fermentable Dietary Fiber	12:36	Fermentable Dietary Fiber	12:36	Prebiotics, Fermentable Dietary Fiber, and Health Claims.
26722809	4	0	theme	solid	649:653	arg1	loadings					655:662	elevated solid loadings	640:662	elevated solid loadings of PS	640:668	Water holding capacity and viscosity of the PS influenced ethanol production at elevated solid loadings of PS.
26722809	1	1	theme	Paper	120:124	arg1	PS					134:135	PS	134:135	PS	134:135	Paper sludge (PS) from the paper and pulp industry consists primarily of cellulose and ash and has significant potential for ethanol production.
26722809	1	1	theme	Paper	120:124	arg1	sludge					126:131	Paper sludge	120:131	Paper sludge (PS) from the paper and pulp industry	120:169	Paper sludge (PS) from the paper and pulp industry consists primarily of cellulose and ash and has significant potential for ethanol production.
26722809	5	2	theme	virgin	697:702	arg1	mills					709:713	virgin pulp mills	697:713	virgin pulp mills	697:713	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	0	3	theme	virgin	47:52	arg1	sludge					71:76	virgin and recycle mill sludge	47:76	virgin and recycle mill sludge	47:76	Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation.
26722809	3	4	theme	PS	472:473	arg1	SSF					464:466	SSF	464:466	SSF	464:466	Simultaneous saccharification and fermentation (SSF) of PS in fed-batch culture was investigated at high solid loadings and low enzyme dosages.
26722809	3	4	theme	PS	472:473	arg1	fermentation					450:461	fermentation	450:461	fermentation (SSF)	450:467	Simultaneous saccharification and fermentation (SSF) of PS in fed-batch culture was investigated at high solid loadings and low enzyme dosages.
26722809	3	4	theme	PS	472:473	arg1	saccharification					429:444	Simultaneous saccharification	416:444	Simultaneous saccharification	416:444	Simultaneous saccharification and fermentation (SSF) of PS in fed-batch culture was investigated at high solid loadings and low enzyme dosages.
26722809	5	5	theme	solid	826:830	arg1	loading					832:838	an optimal solid loading	815:838	an optimal solid loading of 27% (w/w)	815:851	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	0	6	theme	mill	66:69	arg1	sludge					71:76	virgin and recycle mill sludge	47:76	virgin and recycle mill sludge	47:76	Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation.
26722809	5	7	theme	%	845:845	arg1	loading					832:838	an optimal solid loading	815:838	an optimal solid loading of 27% (w/w)	815:851	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	0	8	theme	recycle	58:64	arg1	sludge					71:76	virgin and recycle mill sludge	47:76	virgin and recycle mill sludge	47:76	Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation.
26722809	0	9	theme	Paper	0:4	arg1	PS					14:15	PS	14:15	PS	14:15	Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation.
26722809	0	9	theme	Paper	0:4	arg1	sludge					6:11	Paper sludge	0:11	Paper sludge (PS) to bioethanol	0:30	Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation.
26722809	0	9	theme	Paper	0:4	arg1	fermentation					106:117	high-solids fermentation	94:117	high-solids fermentation	94:117	Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation.
26722809	5	10	theme	optimal	818:824	arg1	loading					832:838	an optimal solid loading	815:838	an optimal solid loading of 27% (w/w)	815:851	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	6	11	theme	pulp	956:959	arg1	yield					940:944	yield	940:944	yield	940:944	Ethanol concentration and yield of virgin pulp and corrugated recycle PS were 34.2g/L at 66.9% and 45.5 g/L at 78.2%, respectively.
26722809	6	11	theme	pulp	956:959	arg1	concentration					922:934	Ethanol concentration	914:934	Ethanol concentration	914:934	Ethanol concentration and yield of virgin pulp and corrugated recycle PS were 34.2g/L at 66.9% and 45.5 g/L at 78.2%, respectively.
26722809	2	12	theme	chemical	361:368	arg1	composition					370:380	chemical composition	361:380	chemical composition	361:380	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	2	13	theme	large	342:346	arg1	variation					348:356	large variation	342:356	large variation in chemical composition and resulting ethanol production	342:413	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	0	14	theme	sludge	71:76	arg1	Evaluation					33:42	Evaluation	33:42	Evaluation of virgin and recycle mill sludge for low enzyme	33:91	Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation.
26722809	3	15	from	fermentation	450:461	arg1	culture					488:494	fed-batch culture	478:494	fed-batch culture	478:494	Simultaneous saccharification and fermentation (SSF) of PS in fed-batch culture was investigated at high solid loadings and low enzyme dosages.
26722809	1	16	contain	has	215:217	arg2	potential					231:239	significant potential	219:239	significant potential for ethanol production	219:262	Paper sludge (PS) from the paper and pulp industry consists primarily of cellulose and ash and has significant potential for ethanol production.
26722809	1	16	contain	has	215:217	arg1	PS					134:135	PS	134:135	PS	134:135	Paper sludge (PS) from the paper and pulp industry consists primarily of cellulose and ash and has significant potential for ethanol production.
26722809	1	16	contain	has	215:217	arg1	sludge					126:131	Paper sludge	120:131	Paper sludge (PS) from the paper and pulp industry	120:169	Paper sludge (PS) from the paper and pulp industry consists primarily of cellulose and ash and has significant potential for ethanol production.
26722809	6	17	theme	virgin	949:954	arg1	pulp					956:959	virgin pulp	949:959	virgin pulp	949:959	Ethanol concentration and yield of virgin pulp and corrugated recycle PS were 34.2g/L at 66.9% and 45.5 g/L at 78.2%, respectively.
26722809	5	18	from	FPU/gdPS	904:911	arg1	PS					895:896	corrugated recycle mill PS	871:896	corrugated recycle mill PS at 11 FPU/gdPS	871:911	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	2	19	theme	Thirty-seven	265:276	arg1	samples					281:287	Thirty-seven PS samples	265:287	Thirty-seven PS samples from 11 South African paper and pulp mills	265:330	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	5	20	theme	FPU/gram	783:790	arg1	gdPS					800:803	gdPS	800:803	gdPS	800:803	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	5	20	theme	FPU/gram	783:790	arg1	PS					796:797	20 FPU/gram dry PS	780:797	20 FPU/gram dry PS (gdPS)	780:804	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	2	21	theme	ethanol	396:402	arg1	production					404:413	resulting ethanol production	386:413	resulting ethanol production	386:413	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	5	22	theme	High	671:674	arg1	viscosity					676:684	High viscosity	671:684	High viscosity of PS from virgin pulp mills	671:713	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	5	23	from	mills	709:713	arg1	PS					689:690	PS	689:690	PS from virgin pulp mills	689:713	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	5	23	from	mills	709:713	arg1	viscosity					676:684	High viscosity	671:684	High viscosity of PS from virgin pulp mills	671:713	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	2	24	theme	pulp	321:324	arg1	mills					326:330	11 South African paper and pulp mills	294:330	mills	326:330	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	2	25	theme	resulting	386:394	arg1	production					404:413	resulting ethanol production	386:413	resulting ethanol production	386:413	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	1	26	theme	significant	219:229	arg1	potential					231:239	significant potential	219:239	significant potential for ethanol production	219:262	Paper sludge (PS) from the paper and pulp industry consists primarily of cellulose and ash and has significant potential for ethanol production.
26722809	5	27	dep	restricted	715:724	arg1	whereas					807:813	whereas	807:813	whereas	807:813	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	2	28	from	paper	311:315	arg1	samples					281:287	Thirty-seven PS samples	265:287	Thirty-seven PS samples from 11 South African paper and pulp mills	265:330	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	3	29	theme	high	516:519	arg1	loadings					527:534	high solid loadings	516:534	high solid loadings	516:534	Simultaneous saccharification and fermentation (SSF) of PS in fed-batch culture was investigated at high solid loadings and low enzyme dosages.
26722809	6	30	theme	Ethanol	914:920	arg1	concentration					922:934	Ethanol concentration	914:934	Ethanol concentration	914:934	Ethanol concentration and yield of virgin pulp and corrugated recycle PS were 34.2g/L at 66.9% and 45.5 g/L at 78.2%, respectively.
26722809	6	31	theme	recycle	976:982	arg1	PS					984:985	corrugated recycle PS	965:985	corrugated recycle PS	965:985	Ethanol concentration and yield of virgin pulp and corrugated recycle PS were 34.2g/L at 66.9% and 45.5 g/L at 78.2%, respectively.
26722809	0	32	theme	low	82:84	arg1	enzyme					86:91	low enzyme	82:91	low enzyme	82:91	Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation.
26722809	3	33	theme	solid	521:525	arg1	loadings					527:534	high solid loadings	516:534	high solid loadings	516:534	Simultaneous saccharification and fermentation (SSF) of PS in fed-batch culture was investigated at high solid loadings and low enzyme dosages.
26722809	1	34	theme	paper	147:151	arg1	industry					162:169	the paper and pulp industry	143:169	industry	162:169	Paper sludge (PS) from the paper and pulp industry consists primarily of cellulose and ash and has significant potential for ethanol production.
26722809	5	35	theme	solid	730:734	arg1	loading					736:742	the solid loading	726:742	the solid loading	726:742	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	0	36	theme	high-solids	94:104	arg1	sludge					6:11	Paper sludge	0:11	Paper sludge (PS) to bioethanol	0:30	Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation.
26722809	0	36	theme	high-solids	94:104	arg1	fermentation					106:117	high-solids fermentation	94:117	high-solids fermentation	94:117	Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation.
26722809	5	37	theme	enzyme	763:768	arg1	dosage					770:775	an enzyme dosage	760:775	an enzyme dosage of 20 FPU/gram dry PS (gdPS)	760:804	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	5	38	theme	recycle	882:888	arg1	PS					895:896	corrugated recycle mill PS	871:896	corrugated recycle mill PS at 11 FPU/gdPS	871:911	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	5	39	theme	dry	792:794	arg1	gdPS					800:803	gdPS	800:803	gdPS	800:803	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	5	39	theme	dry	792:794	arg1	PS					796:797	20 FPU/gram dry PS	780:797	20 FPU/gram dry PS (gdPS)	780:804	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	4	40	theme	PS	604:605	arg1	viscosity					587:595	viscosity	587:595	viscosity	587:595	Water holding capacity and viscosity of the PS influenced ethanol production at elevated solid loadings of PS.
26722809	4	40	theme	PS	604:605	arg1	capacity					574:581	Water holding capacity	560:581	Water holding capacity	560:581	Water holding capacity and viscosity of the PS influenced ethanol production at elevated solid loadings of PS.
26722809	4	41	theme	ethanol	618:624	arg1	production					626:635	ethanol production	618:635	ethanol production	618:635	Water holding capacity and viscosity of the PS influenced ethanol production at elevated solid loadings of PS.
26722809	1	42	theme	ethanol	245:251	arg1	production					253:262	ethanol production	245:262	ethanol production	245:262	Paper sludge (PS) from the paper and pulp industry consists primarily of cellulose and ash and has significant potential for ethanol production.
26722809	5	43	from	viscosity	676:684	arg1	mills					709:713	virgin pulp mills	697:713	virgin pulp mills	697:713	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	5	44	theme	mill	890:893	arg1	PS					895:896	corrugated recycle mill PS	871:896	corrugated recycle mill PS at 11 FPU/gdPS	871:911	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	2	45	from	mills	326:330	arg1	samples					281:287	Thirty-seven PS samples	265:287	Thirty-seven PS samples from 11 South African paper and pulp mills	265:330	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	1	46	theme	pulp	157:160	arg1	industry					162:169	the paper and pulp industry	143:169	industry	162:169	Paper sludge (PS) from the paper and pulp industry consists primarily of cellulose and ash and has significant potential for ethanol production.
26722809	5	47	theme	PS	796:797	arg1	dosage					770:775	an enzyme dosage	760:775	an enzyme dosage of 20 FPU/gram dry PS (gdPS)	760:804	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	3	48	theme	low	540:542	arg1	dosages					551:557	low enzyme dosages	540:557	low enzyme dosages	540:557	Simultaneous saccharification and fermentation (SSF) of PS in fed-batch culture was investigated at high solid loadings and low enzyme dosages.
26722809	6	49	theme	corrugated	965:974	arg1	PS					984:985	corrugated recycle PS	965:985	corrugated recycle PS	965:985	Ethanol concentration and yield of virgin pulp and corrugated recycle PS were 34.2g/L at 66.9% and 45.5 g/L at 78.2%, respectively.
26722809	3	50	theme	enzyme	544:549	arg1	dosages					551:557	low enzyme dosages	540:557	low enzyme dosages	540:557	Simultaneous saccharification and fermentation (SSF) of PS in fed-batch culture was investigated at high solid loadings and low enzyme dosages.
26722809	3	51	from	saccharification	429:444	arg1	culture					488:494	fed-batch culture	478:494	fed-batch culture	478:494	Simultaneous saccharification and fermentation (SSF) of PS in fed-batch culture was investigated at high solid loadings and low enzyme dosages.
26722809	4	52	theme	PS	667:668	arg1	loadings					655:662	elevated solid loadings	640:662	elevated solid loadings of PS	640:668	Water holding capacity and viscosity of the PS influenced ethanol production at elevated solid loadings of PS.
26722809	1	53	from	industry	162:169	arg1	PS					134:135	PS	134:135	PS	134:135	Paper sludge (PS) from the paper and pulp industry consists primarily of cellulose and ash and has significant potential for ethanol production.
26722809	1	53	from	industry	162:169	arg1	sludge					126:131	Paper sludge	120:131	Paper sludge (PS) from the paper and pulp industry	120:169	Paper sludge (PS) from the paper and pulp industry consists primarily of cellulose and ash and has significant potential for ethanol production.
26722809	4	54	theme	elevated	640:647	arg1	loadings					655:662	elevated solid loadings	640:662	elevated solid loadings of PS	640:668	Water holding capacity and viscosity of the PS influenced ethanol production at elevated solid loadings of PS.
26722809	6	55	theme	PS	984:985	arg1	yield					940:944	yield	940:944	yield	940:944	Ethanol concentration and yield of virgin pulp and corrugated recycle PS were 34.2g/L at 66.9% and 45.5 g/L at 78.2%, respectively.
26722809	6	55	theme	PS	984:985	arg1	concentration					922:934	Ethanol concentration	914:934	Ethanol concentration	914:934	Ethanol concentration and yield of virgin pulp and corrugated recycle PS were 34.2g/L at 66.9% and 45.5 g/L at 78.2%, respectively.
26722809	4	56	theme	holding	566:572	arg1	capacity					574:581	Water holding capacity	560:581	Water holding capacity	560:581	Water holding capacity and viscosity of the PS influenced ethanol production at elevated solid loadings of PS.
26722809	2	57	theme	PS	278:279	arg1	samples					281:287	Thirty-seven PS samples	265:287	Thirty-seven PS samples from 11 South African paper and pulp mills	265:330	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	6	58	from	%	1029:1029	arg1	%					1007:1007	66.9%	1003:1007	66.9%	1003:1007	Ethanol concentration and yield of virgin pulp and corrugated recycle PS were 34.2g/L at 66.9% and 45.5 g/L at 78.2%, respectively.
26722809	6	58	from	%	1029:1029	arg1	g/L					1018:1020	45.5 g/L	1013:1020	45.5 g/L at 78.2%	1013:1029	Ethanol concentration and yield of virgin pulp and corrugated recycle PS were 34.2g/L at 66.9% and 45.5 g/L at 78.2%, respectively.
26722809	4	59	theme	Water	560:564	arg1	capacity					574:581	Water holding capacity	560:581	Water holding capacity	560:581	Water holding capacity and viscosity of the PS influenced ethanol production at elevated solid loadings of PS.
26722809	5	60	theme	pulp	704:707	arg1	mills					709:713	virgin pulp mills	697:713	virgin pulp mills	697:713	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	3	61	theme	Simultaneous	416:427	arg1	saccharification					429:444	Simultaneous saccharification	416:444	Simultaneous saccharification	416:444	Simultaneous saccharification and fermentation (SSF) of PS in fed-batch culture was investigated at high solid loadings and low enzyme dosages.
26722809	0	62	dep	sludge	6:11	arg1	Evaluation					33:42	Evaluation	33:42	Evaluation of virgin and recycle mill sludge for low enzyme	33:91	Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation.
26722809	2	63	from	variation	348:356	arg1	production					404:413	resulting ethanol production	386:413	resulting ethanol production	386:413	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	2	63	from	variation	348:356	arg1	composition					370:380	chemical composition	361:380	chemical composition	361:380	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	2	64	theme	African	303:309	arg1	paper					311:315	11 South African paper and pulp mills	294:330	paper	311:315	Thirty-seven PS samples from 11 South African paper and pulp mills exhibited large variation in chemical composition and resulting ethanol production.
26722809	5	65	theme	corrugated	871:880	arg1	PS					895:896	corrugated recycle mill PS	871:896	corrugated recycle mill PS at 11 FPU/gdPS	871:911	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
26722809	3	66	theme	fed-batch	478:486	arg1	culture					488:494	fed-batch culture	478:494	fed-batch culture	478:494	Simultaneous saccharification and fermentation (SSF) of PS in fed-batch culture was investigated at high solid loadings and low enzyme dosages.
26722809	5	67	theme	PS	689:690	arg1	viscosity					676:684	High viscosity	671:684	High viscosity of PS from virgin pulp mills	671:713	High viscosity of PS from virgin pulp mills restricted the solid loading to 18% (w/w) at an enzyme dosage of 20 FPU/gram dry PS (gdPS), whereas an optimal solid loading of 27% (w/w) was achieved with corrugated recycle mill PS at 11 FPU/gdPS.
29270873	5	0	dep	%	1718:1718	arg1	similarities					1720:1731	similarities	1720:1731	98.5 and 97.9% similarities of C10 to A. succiniciruminis	1705:1761	Comparative analyses using rpsA and rplB showed 98.5 and 97.9% similarities of C10 to A. succiniciruminis, respectively, and 97.5 and 97.6% similarities to A. glycerinitolerans, respectively.
29270873	5	0	dep	%	1718:1718	arg1	similarities					1797:1808	similarities	1797:1808	similarities	1797:1808	Comparative analyses using rpsA and rplB showed 98.5 and 97.9% similarities of C10 to A. succiniciruminis, respectively, and 97.5 and 97.6% similarities to A. glycerinitolerans, respectively.
29270873	1	1	dep	deer	422:425	arg1	elaphus					435:441	Cervus elaphus	428:441	Cervus elaphus	428:441	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	0	2	theme	described	142:150	arg1	ruminicola					219:228	ruminicola	219:228	ruminicola	219:228	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	0	2	theme	described	142:150	arg1	succiniciruminis					172:187	succiniciruminis	172:187	succiniciruminis	172:187	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	0	2	theme	described	142:150	arg1	glycerinitolerans					193:209	glycerinitolerans	193:209	glycerinitolerans	193:209	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	0	2	theme	described	142:150	arg1	species					152:158	the recently described species	129:158	the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola	129:228	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	4	3	theme	rplB	1478:1481	arg1	sequences					1497:1505	rplB (597 bp) gene sequences	1478:1505	rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains	1478:1654	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	3	4	dep	27982T	1198:1203	arg1	%					1210:1210	97.2%	1206:1210	97.2%	1206:1210	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	5	theme	ruminicola	1183:1192	arg1	27982T					1198:1203	the Actinomyces ruminicola DSM 27982T	1167:1203	the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb)	1167:1249	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	4	6	dep	species	1313:1319	arg1	succiniciruminis					1324:1339	A. succiniciruminis	1321:1339	A. succiniciruminis	1321:1339	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	4	6	dep	species	1313:1319	arg1	glycerinitolerans					1348:1364	A. glycerinitolerans	1345:1364	A. glycerinitolerans	1345:1364	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	2	7	theme	M10	623:625	arg1	medium					627:632	The anaerobic M10 medium	609:632	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon	609:727	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	1	8	with	C10	313:315	arg1	branching					333:341	occasional branching	322:341	occasional branching	322:341	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	5	9	theme	Comparative	1657:1667	arg1	analyses					1669:1676	Comparative analyses	1657:1676	Comparative analyses using rpsA and rplB	1657:1696	Comparative analyses using rpsA and rplB showed 98.5 and 97.9% similarities of C10 to A. succiniciruminis, respectively, and 97.5 and 97.6% similarities to A. glycerinitolerans, respectively.
29270873	3	10	theme	41468 pb	1241:1248	arg1	fragment					1229:1236	all compared fragment	1216:1236	all compared fragment of 41468 pb	1216:1248	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	4	11	from	phylogeny	1615:1623	arg1	applicable					1564:1573	applicable	1564:1573	applicable	1564:1573	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	3	12	from	%	1210:1210	arg1	fragment					1229:1236	all compared fragment	1216:1236	all compared fragment of 41468 pb	1216:1248	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	2	13	contain	containing	634:643	arg1	medium					627:632	The anaerobic M10 medium	609:632	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon	609:727	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	2	13	contain	containing	634:643	arg2	carboxymethylcellulose					668:689	carboxymethylcellulose	668:689	carboxymethylcellulose	668:689	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	2	13	contain	containing	634:643	arg2	fluid					658:662	fluid	658:662	fluid	658:662	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	2	13	contain	containing	634:643	arg2	source					704:709	a defined source	694:709	a defined source of organic carbon	694:727	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	3	14	theme	complete	1042:1049	arg1	genome					1051:1056	the complete genome	1038:1056	the complete genome	1038:1056	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	5	15	theme	C10	1736:1738	arg1	%					1718:1718	98.5 and 97.9%	1705:1718	98.5 and 97.9% similarities of C10 to A. succiniciruminis	1705:1761	Comparative analyses using rpsA and rplB showed 98.5 and 97.9% similarities of C10 to A. succiniciruminis, respectively, and 97.5 and 97.6% similarities to A. glycerinitolerans, respectively.
29270873	5	15	theme	C10	1736:1738	arg1	C10					1736:1738	C10	1736:1738	C10 to A. succiniciruminis	1736:1761	Comparative analyses using rpsA and rplB showed 98.5 and 97.9% similarities of C10 to A. succiniciruminis, respectively, and 97.5 and 97.6% similarities to A. glycerinitolerans, respectively.
29270873	5	15	theme	C10	1736:1738	arg1	%					1795:1795	97.5 and 97.6%	1782:1795	%	1795:1795	Comparative analyses using rpsA and rplB showed 98.5 and 97.9% similarities of C10 to A. succiniciruminis, respectively, and 97.5 and 97.6% similarities to A. glycerinitolerans, respectively.
29270873	4	16	dep	rpsA	1450:1453	arg1	length					1456:1461	length	1456:1461	length of 519 bp	1456:1471	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	2	17	theme	bacterial	756:764	arg1	isolation					766:774	bacterial isolation	756:774	bacterial isolation	756:774	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	10	18	theme	red	2478:2480	arg1	deer					2482:2485	a wild red deer	2471:2485	a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities	2471:2595	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	5	19	theme	97.6	1791:1794	arg1	C10					1736:1738	C10	1736:1738	C10 to A. succiniciruminis	1736:1761	Comparative analyses using rpsA and rplB showed 98.5 and 97.9% similarities of C10 to A. succiniciruminis, respectively, and 97.5 and 97.6% similarities to A. glycerinitolerans, respectively.
29270873	5	19	theme	97.6	1791:1794	arg1	%					1795:1795	97.5 and 97.6%	1782:1795	%	1795:1795	Comparative analyses using rpsA and rplB showed 98.5 and 97.9% similarities of C10 to A. succiniciruminis, respectively, and 97.5 and 97.6% similarities to A. glycerinitolerans, respectively.
29270873	1	20	from	tract	562:566	arg1	Republic					599:606	the Czech Republic	589:606	the Czech Republic	589:606	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	0	21	theme	related	118:124	arg1	deer					88:91	a red deer	82:91	a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola	82:228	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	6	22	theme	evaluated	1889:1897	arg1	100236 = LMG					1917:1928	=DSM 100236 = LMG 28777	1912:1934	=DSM 100236 = LMG 28777	1912:1934	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	6	22	theme	evaluated	1889:1897	arg1	little					1942:1947	little	1942:1947	little	1942:1947	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	6	22	theme	evaluated	1889:1897	arg1	C10					1907:1909	the evaluated isolate C10	1885:1909	the evaluated isolate C10 (=DSM 100236 = LMG 28777)	1885:1935	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	10	23	theme	xylanolytic	2574:2584	arg1	activities					2586:2595	noteworthy cellulolytic and xylanolytic activities	2546:2595	noteworthy cellulolytic and xylanolytic activities	2546:2595	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	3	24	theme	gene	790:793	arg1	similarity					795:804	The 16S rRNA gene similarity	777:804	The 16S rRNA gene similarity	777:804	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	1	25	theme	deer	422:425	arg1	fluid					407:411	the rumen fluid	397:411	the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic	397:606	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	8	26	theme	bacterial	2208:2216	arg1	strain					2218:2223	the bacterial strain	2204:2223	the bacterial strain	2204:2223	Unfortunately, they did not allow the bacterial strain to be classified into a particular species.
29270873	10	27	theme	Actinomyces	2525:2535	arg1	species					2537:2543	Actinomyces species	2525:2543	Actinomyces species	2525:2543	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	3	28	theme	16S	781:783	arg1	similarity					795:804	The 16S rRNA gene similarity	777:804	The 16S rRNA gene similarity	777:804	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	5	29	theme	97.5	1782:1785	arg1	C10					1736:1738	C10	1736:1738	C10 to A. succiniciruminis	1736:1761	Comparative analyses using rpsA and rplB showed 98.5 and 97.9% similarities of C10 to A. succiniciruminis, respectively, and 97.5 and 97.6% similarities to A. glycerinitolerans, respectively.
29270873	5	29	theme	97.5	1782:1785	arg1	%					1795:1795	97.5 and 97.6%	1782:1795	%	1795:1795	Comparative analyses using rpsA and rplB showed 98.5 and 97.9% similarities of C10 to A. succiniciruminis, respectively, and 97.5 and 97.6% similarities to A. glycerinitolerans, respectively.
29270873	3	30	theme	accession	893:901	arg1	Am4T					879:882	Am4T	879:882	Am4T	879:882	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	30	theme	accession	893:901	arg1	number					903:908	GenBank accession number	885:908	GenBank accession number of the gene retrieved from the complete genome	885:955	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	2	31	theme	organic	714:720	arg1	carbon					722:727	organic carbon	714:727	organic carbon	714:727	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	10	32	theme	view	2517:2520	arg1	point					2508:2512	the point	2504:2512	the point of view of Actinomyces species	2504:2543	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	3	33	theme	characterized	824:836	arg1	relatives					1090:1098	the closest relatives	1078:1098	the closest relatives (99.7 and 99.6% gene pairwise identity, respectively)	1078:1152	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	33	theme	characterized	824:836	arg1	species					842:848	recently characterized new species	815:848	recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017)	815:1073	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	33	theme	characterized	824:836	arg1	Am4T					879:882	Am4T	879:882	Am4T	879:882	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	33	theme	characterized	824:836	arg1	G10T					1002:1005	G10T	1002:1005	G10T	1002:1005	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	6	34	theme	cow	2044:2046	arg1	rumen					2015:2019	the rumen	2011:2019	the rumen of a Holstein-Friesian cow than A. glycerinitolerans	2011:2072	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	3	35	dep	species	842:848	arg1	number					1026:1031	GenBank accession number	1008:1031	GenBank accession number from the complete genome	1008:1056	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	35	dep	species	842:848	arg1	species					842:848	recently characterized new species	815:848	recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017)	815:1073	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	35	dep	species	842:848	arg1	Am4T					879:882	Am4T	879:882	Am4T	879:882	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	35	dep	species	842:848	arg1	G10T					1002:1005	G10T	1002:1005	G10T	1002:1005	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	35	dep	species	842:848	arg1	number					903:908	GenBank accession number	885:908	GenBank accession number of the gene retrieved from the complete genome	885:955	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	10	36	theme	detailed	2383:2390	arg1	characterization					2403:2418	detailed phenotypic characterization	2383:2418	detailed phenotypic characterization of bacterial strain C10	2383:2442	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	2	37	theme	bovine	645:650	arg1	source					704:709	a defined source	694:709	a defined source of organic carbon	694:727	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	2	37	theme	bovine	645:650	arg1	carboxymethylcellulose					668:689	carboxymethylcellulose	668:689	carboxymethylcellulose	668:689	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	2	37	theme	bovine	645:650	arg1	fluid					658:662	fluid	658:662	fluid	658:662	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	10	38	theme	cellulolytic	2557:2568	arg1	activities					2586:2595	noteworthy cellulolytic and xylanolytic activities	2546:2595	noteworthy cellulolytic and xylanolytic activities	2546:2595	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	0	39	dep	deer	88:91	arg1	elaphus					101:107	Cervus elaphus	94:107	Cervus elaphus	94:107	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	3	40	theme	gene	917:920	arg1	Am4T					879:882	Am4T	879:882	Am4T	879:882	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	40	theme	gene	917:920	arg1	number					903:908	GenBank accession number	885:908	GenBank accession number of the gene retrieved from the complete genome	885:955	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	0	41	theme	deer	88:91	arg1	rumen					73:77	the rumen	69:77	the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola	69:228	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	10	42	theme	bacterial	2423:2431	arg1	C10					2440:2442	bacterial strain C10	2423:2442	bacterial strain C10	2423:2442	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	4	43	theme	Actinomyces	1628:1638	arg1	species/strains					1640:1654	Actinomyces species/strains	1628:1654	Actinomyces species/strains	1628:1654	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	1	44	theme	digestive	552:560	arg1	tract					562:566	the digestive tract	548:566	the digestive tract of wild ruminants in the Czech Republic	548:606	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	4	45	theme	519 bp	1466:1471	arg1	length					1456:1461	length	1456:1461	length of 519 bp	1456:1471	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	1	46	theme	oxidase-negative	265:280	arg1	C10					313:315	oxidase-negative and short rod-shaped bacterium C10	265:315	oxidase-negative and short rod-shaped bacterium C10	265:315	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	3	47	theme	complete	941:948	arg1	genome					950:955	the complete genome	937:955	the complete genome	937:955	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	48	theme	accession	1016:1024	arg1	number					1026:1031	GenBank accession number	1008:1031	GenBank accession number from the complete genome	1008:1056	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	48	theme	accession	1016:1024	arg1	G10T					1002:1005	G10T	1002:1005	G10T	1002:1005	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	6	49	theme	=DSM	1912:1915	arg1	100236 = LMG					1917:1928	=DSM 100236 = LMG 28777	1912:1934	=DSM 100236 = LMG 28777	1912:1934	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	6	49	theme	=DSM	1912:1915	arg1	C10					1907:1909	the evaluated isolate C10	1885:1909	the evaluated isolate C10 (=DSM 100236 = LMG 28777)	1885:1935	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	1	50	theme	short	286:290	arg1	C10					313:315	oxidase-negative and short rod-shaped bacterium C10	265:315	oxidase-negative and short rod-shaped bacterium C10	265:315	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	3	51	theme	gene	1116:1119	arg1	identity					1130:1137	gene pairwise identity	1116:1137	99.7 and 99.6% gene pairwise identity	1101:1137	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	1	52	theme	ruminants	576:584	arg1	tract					562:566	the digestive tract	548:566	the digestive tract of wild ruminants in the Czech Republic	548:606	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	4	53	theme	rpsA	1450:1453	arg1	regions					1439:1445	variable regions	1430:1445	variable regions of rpsA (length of 519 bp)	1430:1472	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	4	53	theme	rpsA	1450:1453	arg1	sequences					1497:1505	rplB (597 bp) gene sequences	1478:1505	rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains	1478:1654	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	3	54	dep	relatives	1090:1098	arg1	%					1114:1114	99.7 and 99.6%	1101:1114	99.7 and 99.6% gene pairwise identity	1101:1137	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	1	55	theme	bacterium	303:311	arg1	C10					313:315	oxidase-negative and short rod-shaped bacterium C10	265:315	oxidase-negative and short rod-shaped bacterium C10	265:315	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	0	56	theme	bacterial	34:42	arg1	C10					51:53	a xylanolytic bacterial strain C10	20:53	a xylanolytic bacterial strain C10	20:53	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	1	57	with	catalase	252:259	arg1	branching					333:341	occasional branching	322:341	occasional branching	322:341	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	10	58	theme	C10	2440:2442	arg1	classification					2364:2377	classification	2364:2377	classification	2364:2377	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	10	58	theme	C10	2440:2442	arg1	characterization					2403:2418	detailed phenotypic characterization	2383:2418	detailed phenotypic characterization of bacterial strain C10	2383:2442	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	4	59	from	classification	1595:1608	arg1	applicable					1564:1573	applicable	1564:1573	applicable	1564:1573	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	0	60	theme	C10	51:53	arg1	Characterization					0:15	Characterization	0:15	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.	0:229	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	1	61	with	Gram-stain-positive	231:249	arg1	branching					333:341	occasional branching	322:341	occasional branching	322:341	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	4	62	from	differentiation	1578:1592	arg1	applicable					1564:1573	applicable	1564:1573	applicable	1564:1573	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	7	63	theme	strain	2119:2124	arg1	C10					2126:2128	strain C10	2119:2128	strain C10	2119:2128	Phylogenetic analyses confirmed affinity of strain C10 to both recently characterized species.
29270873	4	64	from	applicable	1564:1573	arg1	classification					1595:1608	classification	1595:1608	classification	1595:1608	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	4	64	from	applicable	1564:1573	arg1	phylogeny					1615:1623	phylogeny	1615:1623	phylogeny	1615:1623	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	4	64	from	applicable	1564:1573	arg1	differentiation					1578:1592	differentiation	1578:1592	differentiation	1578:1592	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	9	65	theme	similar	2307:2313	arg1	conclusions					2315:2325	similar conclusions	2307:2325	similar conclusions	2307:2325	Phenotypic characterization suggested similar conclusions.
29270873	4	66	theme	examined	1289:1296	arg1	strain					1298:1303	the examined strain	1285:1303	the examined strain	1285:1303	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	0	67	theme	species	152:158	arg1	deer					88:91	a red deer	82:91	a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola	82:228	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	1	68	theme	rumen	522:526	arg1	bacteria					528:535	new xylanolytic and cellulolytic rumen bacteria	489:535	bacteria	528:535	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	3	69	theme	DSM	1194:1196	arg1	27982T					1198:1203	the Actinomyces ruminicola DSM 27982T	1167:1203	the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb)	1167:1249	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	70	theme	GenBank	1008:1014	arg1	number					1026:1031	GenBank accession number	1008:1031	GenBank accession number from the complete genome	1008:1056	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	70	theme	GenBank	1008:1014	arg1	G10T					1002:1005	G10T	1002:1005	G10T	1002:1005	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	4	71	theme	taxonomic	1263:1271	arg1	affinity					1273:1280	the taxonomic affinity	1259:1280	the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans	1259:1364	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	3	72	theme	Actinomyces	1171:1181	arg1	27982T					1198:1203	the Actinomyces ruminicola DSM 27982T	1167:1203	the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb)	1167:1249	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	2	73	theme	anaerobic	613:621	arg1	medium					627:632	The anaerobic M10 medium	609:632	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon	609:727	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	1	74	theme	study	461:465	arg1	course					451:456	the course	447:456	the course of study	447:465	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	4	75	theme	specific	1526:1533	arg1	primers					1535:1541	specific primers	1526:1541	specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains	1526:1654	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	2	76	theme	isolation	766:774	arg1	process					745:751	the process	741:751	the process of bacterial isolation	741:774	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	7	77	theme	Phylogenetic	2075:2086	arg1	analyses					2088:2095	Phylogenetic analyses	2075:2095	Phylogenetic analyses	2075:2095	Phylogenetic analyses confirmed affinity of strain C10 to both recently characterized species.
29270873	9	78	theme	Phenotypic	2269:2278	arg1	characterization					2280:2295	Phenotypic characterization	2269:2295	Phenotypic characterization	2269:2295	Phenotypic characterization suggested similar conclusions.
29270873	1	79	theme	rumen	401:405	arg1	fluid					407:411	the rumen fluid	397:411	the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic	397:606	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	0	80	dep	species	152:158	arg1	ruminicola					219:228	ruminicola	219:228	ruminicola	219:228	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	0	80	dep	species	152:158	arg1	succiniciruminis					172:187	succiniciruminis	172:187	succiniciruminis	172:187	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	0	80	dep	species	152:158	arg1	glycerinitolerans					193:209	glycerinitolerans	193:209	glycerinitolerans	193:209	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	0	80	dep	species	152:158	arg1	species					152:158	the recently described species	129:158	the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola	129:228	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	3	81	theme	compared	1220:1227	arg1	fragment					1229:1236	all compared fragment	1216:1236	all compared fragment of 41468 pb	1216:1248	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	4	82	theme	taxonomic	1371:1379	arg1	status					1381:1386	its taxonomic status	1367:1386	its taxonomic status towards these species	1367:1408	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	10	83	theme	wild	2473:2476	arg1	deer					2482:2485	a wild red deer	2471:2485	a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities	2471:2595	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	2	84	theme	defined	696:702	arg1	source					704:709	a defined source	694:709	a defined source of organic carbon	694:727	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	2	84	theme	defined	696:702	arg1	carboxymethylcellulose					668:689	carboxymethylcellulose	668:689	carboxymethylcellulose	668:689	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	2	84	theme	defined	696:702	arg1	fluid					658:662	fluid	658:662	fluid	658:662	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	6	85	theme	isolate	1899:1905	arg1	100236 = LMG					1917:1928	=DSM 100236 = LMG 28777	1912:1934	=DSM 100236 = LMG 28777	1912:1934	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	6	85	theme	isolate	1899:1905	arg1	little					1942:1947	little	1942:1947	little	1942:1947	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	6	85	theme	isolate	1899:1905	arg1	C10					1907:1909	the evaluated isolate C10	1885:1909	the evaluated isolate C10 (=DSM 100236 = LMG 28777)	1885:1935	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	0	86	attach	isolated	55:62	arg1	rumen					73:77	the rumen	69:77	the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola	69:228	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	0	86	attach	isolated	55:62	arg2	Characterization					0:15	Characterization	0:15	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.	0:229	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	1	87	theme	red	418:420	arg1	deer					422:425	a red deer	416:425	a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic	416:606	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	1	88	theme	Czech	593:597	arg1	Republic					599:606	the Czech Republic	589:606	the Czech Republic	589:606	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	2	89	theme	carbon	722:727	arg1	source					704:709	a defined source	694:709	a defined source of organic carbon	694:727	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	2	89	theme	carbon	722:727	arg1	carboxymethylcellulose					668:689	carboxymethylcellulose	668:689	carboxymethylcellulose	668:689	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	2	89	theme	carbon	722:727	arg1	fluid					658:662	fluid	658:662	fluid	658:662	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	3	90	theme	rRNA	785:788	arg1	similarity					795:804	The 16S rRNA gene similarity	777:804	The 16S rRNA gene similarity	777:804	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	91	dep	%	1114:1114	arg1	identity					1130:1137	gene pairwise identity	1116:1137	99.7 and 99.6% gene pairwise identity	1101:1137	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	10	92	theme	brief	2333:2337	arg1	contribution					2339:2350	This brief contribution	2328:2350	This brief contribution	2328:2350	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	4	93	theme	species/strains	1640:1654	arg1	classification					1595:1608	classification	1595:1608	classification	1595:1608	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	4	93	theme	species/strains	1640:1654	arg1	phylogeny					1615:1623	phylogeny	1615:1623	phylogeny	1615:1623	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	4	93	theme	species/strains	1640:1654	arg1	differentiation					1578:1592	differentiation	1578:1592	differentiation	1578:1592	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	2	94	theme	rumen	652:656	arg1	source					704:709	a defined source	694:709	a defined source of organic carbon	694:727	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	2	94	theme	rumen	652:656	arg1	carboxymethylcellulose					668:689	carboxymethylcellulose	668:689	carboxymethylcellulose	668:689	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	2	94	theme	rumen	652:656	arg1	fluid					658:662	fluid	658:662	fluid	658:662	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	6	95	theme	gene	1855:1858	arg1	identities					1860:1869	gene identities	1855:1869	gene identities	1855:1869	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	3	96	theme	new	838:840	arg1	relatives					1090:1098	the closest relatives	1078:1098	the closest relatives (99.7 and 99.6% gene pairwise identity, respectively)	1078:1152	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	96	theme	new	838:840	arg1	species					842:848	recently characterized new species	815:848	recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017)	815:1073	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	96	theme	new	838:840	arg1	Am4T					879:882	Am4T	879:882	Am4T	879:882	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	96	theme	new	838:840	arg1	G10T					1002:1005	G10T	1002:1005	G10T	1002:1005	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	0	97	theme	red	84:86	arg1	deer					88:91	a red deer	82:91	a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola	82:228	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	6	98	theme	Holstein-Friesian	2026:2042	arg1	cow					2044:2046	a Holstein-Friesian cow	2024:2046	a Holstein-Friesian cow than A. glycerinitolerans	2024:2072	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	3	99	theme	GenBank	885:891	arg1	Am4T					879:882	Am4T	879:882	Am4T	879:882	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	99	theme	GenBank	885:891	arg1	number					903:908	GenBank accession number	885:908	GenBank accession number of the gene retrieved from the complete genome	885:955	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	10	100	theme	noteworthy	2546:2555	arg1	activities					2586:2595	noteworthy cellulolytic and xylanolytic activities	2546:2595	noteworthy cellulolytic and xylanolytic activities	2546:2595	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	1	101	attach	isolated	347:354	arg1	fluid					407:411	the rumen fluid	397:411	the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic	397:606	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	1	101	attach	isolated	347:354	arg2	C10					313:315	oxidase-negative and short rod-shaped bacterium C10	265:315	oxidase-negative and short rod-shaped bacterium C10	265:315	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	1	101	attach	isolated	347:354	arg2	catalase					252:259	catalase	252:259	catalase	252:259	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	1	101	attach	isolated	347:354	arg2	Gram-stain-positive					231:249	Gram-stain-positive	231:249	Gram-stain-positive	231:249	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	1	102	theme	anaerobic	371:379	arg1	conditions					381:390	strictly anaerobic conditions	362:390	strictly anaerobic conditions	362:390	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	10	103	theme	deer	2482:2485	arg1	rumen					2462:2466	the rumen	2458:2466	the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities	2458:2595	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	10	104	theme	species	2537:2543	arg1	view					2517:2520	view	2517:2520	view of Actinomyces species	2517:2543	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	3	105	theme	closest	1082:1088	arg1	relatives					1090:1098	the closest relatives	1078:1098	the closest relatives (99.7 and 99.6% gene pairwise identity, respectively)	1078:1152	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	105	theme	closest	1082:1088	arg1	species					842:848	recently characterized new species	815:848	recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017)	815:1073	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	2	106	used	used	733:736	arg2	medium					627:632	The anaerobic M10 medium	609:632	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon	609:727	The anaerobic M10 medium containing bovine rumen fluid and carboxymethylcellulose as a defined source of organic carbon was used in the process of bacterial isolation.
29270873	0	107	theme	xylanolytic	22:32	arg1	C10					51:53	a xylanolytic bacterial strain C10	20:53	a xylanolytic bacterial strain C10	20:53	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	3	108	dep	number	903:908	arg1	LK995506					958:965	LK995506	958:965	LK995506	958:965	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	10	109	theme	phenotypic	2392:2401	arg1	characterization					2403:2418	detailed phenotypic characterization	2383:2418	detailed phenotypic characterization of bacterial strain C10	2383:2442	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	1	110	theme	wild	571:574	arg1	ruminants					576:584	wild ruminants	571:584	wild ruminants	571:584	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	4	111	theme	variable	1430:1437	arg1	regions					1439:1445	variable regions	1430:1445	variable regions of rpsA (length of 519 bp)	1430:1472	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	0	112	theme	strain	44:49	arg1	C10					51:53	a xylanolytic bacterial strain C10	20:53	a xylanolytic bacterial strain C10	20:53	Characterization of a xylanolytic bacterial strain C10 isolated from the rumen of a red deer (Cervus elaphus) closely related of the recently described species Actinomyces succiniciruminis, A. glycerinitolerans, and A. ruminicola.
29270873	1	113	theme	rod-shaped	292:301	arg1	C10					313:315	oxidase-negative and short rod-shaped bacterium C10	265:315	oxidase-negative and short rod-shaped bacterium C10	265:315	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	10	114	attach	isolated	2444:2451	arg2	classification					2364:2377	classification	2364:2377	classification	2364:2377	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	10	114	attach	isolated	2444:2451	arg1	rumen					2462:2466	the rumen	2458:2466	the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities	2458:2595	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	6	115	attach	isolated	1997:2004	arg1	rumen					2015:2019	the rumen	2011:2019	the rumen of a Holstein-Friesian cow than A. glycerinitolerans	2011:2072	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	6	115	attach	isolated	1997:2004	arg2	species					1969:1975	the species	1965:1975	the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans	1965:2072	Thus, gene identities revealed that the evaluated isolate C10 (=DSM 100236 = LMG 28777) is a little more related to the species A. succiniciruminis isolated from the rumen of a Holstein-Friesian cow than A. glycerinitolerans.
29270873	10	116	theme	strain	2433:2438	arg1	C10					2440:2442	bacterial strain C10	2423:2442	bacterial strain C10	2423:2442	This brief contribution is aimed at classification and detailed phenotypic characterization of bacterial strain C10 isolated from the rumen of a wild red deer exhibiting, from the point of view of Actinomyces species, noteworthy cellulolytic and xylanolytic activities.
29270873	7	117	theme	characterized	2147:2159	arg1	species					2161:2167	both recently characterized species	2133:2167	both recently characterized species	2133:2167	Phylogenetic analyses confirmed affinity of strain C10 to both recently characterized species.
29270873	4	118	theme	597 bp	1484:1489	arg1	sequences					1497:1505	rplB (597 bp) gene sequences	1478:1505	rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains	1478:1654	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	1	119	theme	occasional	322:331	arg1	branching					333:341	occasional branching	322:341	occasional branching	322:341	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
29270873	3	120	theme	pairwise	1121:1128	arg1	identity					1130:1137	gene pairwise identity	1116:1137	99.7 and 99.6% gene pairwise identity	1101:1137	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	121	from	genome	1051:1056	arg1	number					1026:1031	GenBank accession number	1008:1031	GenBank accession number from the complete genome	1008:1056	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	3	121	from	genome	1051:1056	arg1	G10T					1002:1005	G10T	1002:1005	G10T	1002:1005	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	4	122	theme	gene	1492:1495	arg1	sequences					1497:1505	rplB (597 bp) gene sequences	1478:1505	rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains	1478:1654	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	3	123	dep	number	1026:1031	arg1	NZFQTT01000017					1059:1072	NZFQTT01000017	1059:1072	NZFQTT01000017	1059:1072	The 16S rRNA gene similarity revealed recently characterized new species Actinomyces succiniciruminis Am4T (GenBank accession number of the gene retrieved from the complete genome: LK995506) and Actinomyces glycerinitolerans G10T (GenBank accession number from the complete genome: NZFQTT01000017) as the closest relatives (99.7 and 99.6% gene pairwise identity, respectively), followed by the Actinomyces ruminicola DSM 27982T (97.2%, in all compared fragment of 41468 pb).
29270873	8	124	theme	particular	2249:2258	arg1	species					2260:2266	a particular species	2247:2266	a particular species	2247:2266	Unfortunately, they did not allow the bacterial strain to be classified into a particular species.
29270873	4	125	theme	strain	1298:1303	arg1	affinity					1273:1280	the taxonomic affinity	1259:1280	the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans	1259:1364	Due to the taxonomic affinity of the examined strain to both species A. succiniciruminis and A. glycerinitolerans, its taxonomic status towards these species was evaluated using variable regions of rpsA (length of 519 bp) and rplB (597 bp) gene sequences amplified based on specific primers designed so as to be applicable in differentiation, classification, and phylogeny of Actinomyces species/strains.
29270873	7	126	theme	C10	2126:2128	arg1	affinity					2107:2114	affinity	2107:2114	affinity of strain C10 to both recently characterized species	2107:2167	Phylogenetic analyses confirmed affinity of strain C10 to both recently characterized species.
29270873	1	127	theme	cellulolytic	509:520	arg1	bacteria					528:535	new xylanolytic and cellulolytic rumen bacteria	489:535	bacteria	528:535	Gram-stain-positive, catalase and oxidase-negative and short rod-shaped bacterium C10 with occasional branching was isolated under strictly anaerobic conditions from the rumen fluid of a red deer (Cervus elaphus) in the course of study attempting to uncover new xylanolytic and cellulolytic rumen bacteria inhabiting the digestive tract of wild ruminants in the Czech Republic.
25175227	4	0	theme	different	622:630	arg1	ratios					649:654	different chitosan/gelatin ratios	622:654	different chitosan/gelatin ratios	622:654	Samples consisting of different chitosan/gelatin ratios and type/amount of ionic crosslinker have been prepared and characterized.
25175227	3	1	theme	drug	540:543	arg1	release					545:551	prolonged drug release	530:551	prolonged drug release	530:551	Therefore, complex systems capable of prolonged drug release and controlled release kinetics were obtained.
25175227	11	2	theme	wound	2117:2121	arg1	management					2123:2132	wound management	2117:2132	wound management	2117:2132	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	1	3	theme	loaded	292:297	arg1	liposomes					299:307	drug loaded liposomes	287:307	drug loaded liposomes composed of phosphatidylcholine	287:339	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	5	4	theme	multilamellar	946:958	arg1	vesicles					960:967	multilamellar vesicles	946:967	multilamellar vesicles (MLVs)	946:974	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	5	4	theme	multilamellar	946:958	arg1	MLVs					970:973	MLVs	970:973	MLVs	970:973	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	6	5	theme	process	1214:1220	arg1	mechanism					1197:1205	the mechanism	1193:1205	the mechanism of the process	1193:1220	The calcein release kinetics of complex systems were compared to simple systems (control hydrogels) and important changes were observed thus proving that the mechanism of the process increases in complexity.
25175227	3	6	theme	release	568:574	arg1	kinetics					576:583	controlled release kinetics	557:583	controlled release kinetics	557:583	Therefore, complex systems capable of prolonged drug release and controlled release kinetics were obtained.
25175227	5	7	theme	small	908:912	arg1	SUVs					936:939	SUVs	936:939	SUVs	936:939	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	5	7	theme	small	908:912	arg1	vesicles					926:933	small unilamellar vesicles	908:933	small unilamellar vesicles (SUVs)	908:940	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	11	8	from	diseases	2143:2150	arg1	forward					2106:2112	forward	2106:2112	forward	2106:2112	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	2	9	theme	main	346:349	arg1	objective					351:359	The main objective	342:359	The main objective	342:359	The main objective was to create a protective layer to stabilize the liposomal surface and to prolong/control the release of drugs from such systems.
25175227	11	10	theme	time	2084:2087	arg1	periods					2073:2079	prolonged periods	2063:2079	prolonged periods of time	2063:2087	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	2	11	theme	protective	377:386	arg1	layer					388:392	a protective layer	375:392	a protective layer	375:392	The main objective was to create a protective layer to stabilize the liposomal surface and to prolong/control the release of drugs from such systems.
25175227	1	12	used	used	249:252	arg2	preparation					98:108	the preparation	94:108	the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine	94:339	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	1	12	used	used	249:252	arg2	matrices					257:264	matrices	257:264	matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine	257:339	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	9	13	attach	released	1659:1666	arg1	hydrogels					1673:1681	hydrogels	1673:1681	hydrogels	1673:1681	When small unilamellar liposomes were tested, calcein have been released from hydrogels predominantly in a free form (due to their unilamellarity related instability even inside the hydrogel) but in a sustained and controllable manner.
25175227	9	13	attach	released	1659:1666	arg2	calcein					1641:1647	calcein	1641:1647	calcein	1641:1647	When small unilamellar liposomes were tested, calcein have been released from hydrogels predominantly in a free form (due to their unilamellarity related instability even inside the hydrogel) but in a sustained and controllable manner.
25175227	11	14	theme	forward	2106:2112	arg1	step					2101:2104	a step	2099:2104	a step forward in wound management and many diseases that request long term and sustained delivery of drugs	2099:2205	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	9	15	theme	free	1702:1705	arg1	form					1707:1710	a free form	1700:1710	a free form (due to their unilamellarity related instability even inside the hydrogel)	1700:1785	When small unilamellar liposomes were tested, calcein have been released from hydrogels predominantly in a free form (due to their unilamellarity related instability even inside the hydrogel) but in a sustained and controllable manner.
25175227	1	16	dep	loaded	292:297	arg1	drug					287:290	drug	287:290	drug	287:290	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	11	17	theme	capable	2030:2036	arg1	Hydrogels					2020:2028	Hydrogels	2020:2028	Hydrogels capable of delivering drugs over prolonged periods of time	2020:2087	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	6	18	theme	complex	1071:1077	arg1	systems					1079:1085	complex systems	1071:1085	complex systems	1071:1085	The calcein release kinetics of complex systems were compared to simple systems (control hydrogels) and important changes were observed thus proving that the mechanism of the process increases in complexity.
25175227	7	19	theme	polymeric	1338:1346	arg1	matrices					1348:1355	polymeric matrices	1338:1355	polymeric matrices	1338:1355	Also, it is demonstrated that liposomes' stability can be greatly improved by inclusion in polymeric matrices.
25175227	8	20	theme	Multilamellar	1358:1370	arg1	liposomes					1372:1380	Multilamellar liposomes	1358:1380	Multilamellar liposomes	1358:1380	Multilamellar liposomes showed a better release behaviour, which indicates that these calcein loaded vesicles remained intact to some extent after release from the matrix, due to their improved stability provided by the multiple layers.
25175227	11	21	from	forward	2106:2112	arg1	management					2123:2132	wound management	2117:2132	wound management	2117:2132	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	11	21	from	forward	2106:2112	arg1	diseases					2143:2150	many diseases	2138:2150	many diseases	2138:2150	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	8	22	dep	loaded	1452:1457	arg1	calcein					1444:1450	calcein	1444:1450	calcein	1444:1450	Multilamellar liposomes showed a better release behaviour, which indicates that these calcein loaded vesicles remained intact to some extent after release from the matrix, due to their improved stability provided by the multiple layers.
25175227	12	23	theme	many	2291:2294	arg1	cancer					2325:2330	cancer	2325:2330	cancer	2325:2330	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	12	23	theme	many	2291:2294	arg1	diseases					2306:2313	many high risk diseases	2291:2313	many high risk diseases including cancer	2291:2330	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	5	24	theme	polymeric	816:824	arg1	hydrogels					826:834	polymeric hydrogels	816:834	polymeric hydrogels	816:834	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	5	25	from	hydrogels	826:834	arg1	release					803:809	calcein (used as a model hydrophilic drug) release	760:809	calcein (used as a model hydrophilic drug) release from polymeric hydrogels	760:834	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	6	26	theme	calcein	1043:1049	arg1	kinetics					1059:1066	The calcein release kinetics	1039:1066	The calcein release kinetics of complex systems	1039:1085	The calcein release kinetics of complex systems were compared to simple systems (control hydrogels) and important changes were observed thus proving that the mechanism of the process increases in complexity.
25175227	8	27	theme	improved	1543:1550	arg1	stability					1552:1560	their improved stability	1537:1560	their improved stability provided by the multiple layers	1537:1592	Multilamellar liposomes showed a better release behaviour, which indicates that these calcein loaded vesicles remained intact to some extent after release from the matrix, due to their improved stability provided by the multiple layers.
25175227	8	28	theme	better	1391:1396	arg1	behaviour					1406:1414	a better release behaviour	1389:1414	a better release behaviour	1389:1414	Multilamellar liposomes showed a better release behaviour, which indicates that these calcein loaded vesicles remained intact to some extent after release from the matrix, due to their improved stability provided by the multiple layers.
25175227	5	29	theme	model	779:783	arg1	drug					797:800	a model hydrophilic drug	777:800	a model hydrophilic drug	777:800	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	1	30	with	crosslinking	160:171	arg1	sodium					197:202	sodium	197:202	sodium	197:202	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	1	30	with	crosslinking	160:171	arg1	glutaraldehyde					178:191	glutaraldehyde	178:191	glutaraldehyde	178:191	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	2	31	from	systems	483:489	arg1	release					456:462	the release	452:462	the release of drugs from such systems	452:489	The main objective was to create a protective layer to stabilize the liposomal surface and to prolong/control the release of drugs from such systems.
25175227	9	32	theme	small	1600:1604	arg1	liposomes					1618:1626	small unilamellar liposomes	1600:1626	small unilamellar liposomes	1600:1626	When small unilamellar liposomes were tested, calcein have been released from hydrogels predominantly in a free form (due to their unilamellarity related instability even inside the hydrogel) but in a sustained and controllable manner.
25175227	6	33	theme	control	1120:1126	arg1	systems					1111:1117	simple systems	1104:1117	simple systems (control hydrogels)	1104:1137	The calcein release kinetics of complex systems were compared to simple systems (control hydrogels) and important changes were observed thus proving that the mechanism of the process increases in complexity.
25175227	6	33	theme	control	1120:1126	arg1	hydrogels					1128:1136	control hydrogels	1120:1136	control hydrogels	1120:1136	The calcein release kinetics of complex systems were compared to simple systems (control hydrogels) and important changes were observed thus proving that the mechanism of the process increases in complexity.
25175227	2	34	theme	such	478:481	arg1	systems					483:489	such systems	478:489	such systems	478:489	The main objective was to create a protective layer to stabilize the liposomal surface and to prolong/control the release of drugs from such systems.
25175227	2	35	theme	liposomal	411:419	arg1	surface					421:427	the liposomal surface	407:427	the liposomal surface	407:427	The main objective was to create a protective layer to stabilize the liposomal surface and to prolong/control the release of drugs from such systems.
25175227	0	36	theme	Modulated	0:8	arg1	release					10:16	Modulated release	0:16	Modulated release from liposomes	0:31	Modulated release from liposomes entrapped in chitosan/gelatin hydrogels.
25175227	1	37	theme	liposomes	299:307	arg1	inclusion					274:282	the inclusion	270:282	the inclusion of drug loaded liposomes composed of phosphatidylcholine	270:339	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	2	38	theme	drugs	467:471	arg1	release					456:462	the release	452:462	the release of drugs from such systems	452:489	The main objective was to create a protective layer to stabilize the liposomal surface and to prolong/control the release of drugs from such systems.
25175227	1	39	theme	chitosan/gelatin	113:128	arg1	hydrogels					130:138	chitosan/gelatin hydrogels	113:138	chitosan/gelatin hydrogels	113:138	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	12	40	used	used	2233:2236	arg2	hydrogels					2214:2222	These hydrogels	2208:2222	These hydrogels	2208:2222	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	12	40	used	used	2233:2236	arg2	replacement					2248:2258	tissue replacement	2241:2258	tissue replacement	2241:2258	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	12	40	used	used	2233:2236	arg2	systems					2280:2286	injectable depot systems	2263:2286	injectable depot systems	2263:2286	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	12	41	from	replacement	2248:2258	arg1	cancer					2325:2330	cancer	2325:2330	cancer	2325:2330	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	12	41	from	replacement	2248:2258	arg1	diseases					2306:2313	many high risk diseases	2291:2313	many high risk diseases including cancer	2291:2330	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	7	42	from	inclusion	1325:1333	arg1	matrices					1348:1355	polymeric matrices	1338:1355	polymeric matrices	1338:1355	Also, it is demonstrated that liposomes' stability can be greatly improved by inclusion in polymeric matrices.
25175227	3	43	theme	capable	519:525	arg1	systems					511:517	complex systems	503:517	complex systems capable of prolonged drug release and controlled release kinetics	503:583	Therefore, complex systems capable of prolonged drug release and controlled release kinetics were obtained.
25175227	0	44	theme	chitosan/gelatin	46:61	arg1	hydrogels					63:71	chitosan/gelatin hydrogels	46:71	chitosan/gelatin hydrogels	46:71	Modulated release from liposomes entrapped in chitosan/gelatin hydrogels.
25175227	12	45	theme	high	2296:2299	arg1	cancer					2325:2330	cancer	2325:2330	cancer	2325:2330	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	12	45	theme	high	2296:2299	arg1	diseases					2306:2313	many high risk diseases	2291:2313	many high risk diseases including cancer	2291:2330	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	3	46	theme	complex	503:509	arg1	systems					511:517	complex systems	503:517	complex systems capable of prolonged drug release and controlled release kinetics	503:583	Therefore, complex systems capable of prolonged drug release and controlled release kinetics were obtained.
25175227	10	47	theme	drug	1896:1899	arg1	release					1901:1907	drug release	1896:1907	drug release	1896:1907	The main applications of the systems obtained are in the area of drug release for tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example).
25175227	3	48	theme	release	545:551	arg1	capable					519:525	capable	519:525	capable	519:525	Therefore, complex systems capable of prolonged drug release and controlled release kinetics were obtained.
25175227	6	49	theme	important	1143:1151	arg1	changes					1153:1159	important changes	1143:1159	important changes	1143:1159	The calcein release kinetics of complex systems were compared to simple systems (control hydrogels) and important changes were observed thus proving that the mechanism of the process increases in complexity.
25175227	3	50	theme	prolonged	530:538	arg1	release					545:551	prolonged drug release	530:551	prolonged drug release	530:551	Therefore, complex systems capable of prolonged drug release and controlled release kinetics were obtained.
25175227	11	51	theme	many	2138:2141	arg1	diseases					2143:2150	many diseases	2138:2150	many diseases	2138:2150	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	10	52	theme	engineering/tissue	1920:1937	arg1	repair					1939:1944	tissue engineering/tissue repair	1913:1944	tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example)	1913:2017	The main applications of the systems obtained are in the area of drug release for tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example).
25175227	3	53	theme	kinetics	576:583	arg1	capable					519:525	capable	519:525	capable	519:525	Therefore, complex systems capable of prolonged drug release and controlled release kinetics were obtained.
25175227	4	54	theme	chitosan/gelatin	632:647	arg1	ratios					649:654	different chitosan/gelatin ratios	622:654	different chitosan/gelatin ratios	622:654	Samples consisting of different chitosan/gelatin ratios and type/amount of ionic crosslinker have been prepared and characterized.
25175227	10	55	theme	drugs	1973:1977	arg1	administration					1955:1968	topical administration	1947:1968	topical administration of drugs for wound therapy - burns, for example	1947:2016	The main applications of the systems obtained are in the area of drug release for tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example).
25175227	3	56	theme	controlled	557:566	arg1	kinetics					576:583	controlled release kinetics	557:583	controlled release kinetics	557:583	Therefore, complex systems capable of prolonged drug release and controlled release kinetics were obtained.
25175227	5	57	theme	unilamellar	914:924	arg1	SUVs					936:939	SUVs	936:939	SUVs	936:939	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	5	57	theme	unilamellar	914:924	arg1	vesicles					926:933	small unilamellar vesicles	908:933	small unilamellar vesicles (SUVs)	908:940	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	11	58	theme	long	2165:2168	arg1	term					2170:2173	long term	2165:2173	long term	2165:2173	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	1	59	theme	sulphate/sodium	204:218	arg1	tripolyphosphate					220:235	sulphate/sodium tripolyphosphate	204:235	sulphate/sodium tripolyphosphate	204:235	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	5	60	theme	calcein	760:766	arg1	release					803:809	calcein (used as a model hydrophilic drug) release	760:809	calcein (used as a model hydrophilic drug) release from polymeric hydrogels	760:834	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	11	61	theme	prolonged	2063:2071	arg1	periods					2073:2079	prolonged periods	2063:2079	prolonged periods of time	2063:2087	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	9	62	theme	related	1741:1747	arg1	instability					1749:1759	their unilamellarity related instability	1720:1759	their unilamellarity related instability even inside the hydrogel	1720:1784	When small unilamellar liposomes were tested, calcein have been released from hydrogels predominantly in a free form (due to their unilamellarity related instability even inside the hydrogel) but in a sustained and controllable manner.
25175227	4	63	theme	crosslinker	681:691	arg1	type/amount					660:670	type/amount	660:670	type/amount of ionic crosslinker	660:691	Samples consisting of different chitosan/gelatin ratios and type/amount of ionic crosslinker have been prepared and characterized.
25175227	4	63	theme	crosslinker	681:691	arg1	ratios					649:654	different chitosan/gelatin ratios	622:654	different chitosan/gelatin ratios	622:654	Samples consisting of different chitosan/gelatin ratios and type/amount of ionic crosslinker have been prepared and characterized.
25175227	10	64	theme	wound	1983:1987	arg1	therapy					1989:1995	wound therapy	1983:1995	wound therapy	1983:1995	The main applications of the systems obtained are in the area of drug release for tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example).
25175227	5	65	theme	calcein	887:893	arg1	inclusion					895:903	calcein inclusion	887:903	calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition	887:1036	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	5	66	dep	release	803:809	arg1	used					769:772	used	769:772	used as a model hydrophilic drug	769:800	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	5	67	with	hydrogels	1002:1010	arg1	composition					1026:1036	variable composition	1017:1036	variable composition	1017:1036	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	11	68	from	management	2123:2132	arg1	forward					2106:2112	forward	2106:2112	forward	2106:2112	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	5	69	theme	present	735:741	arg1	study					743:747	The present study	731:747	The present study	731:747	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	10	70	theme	main	1835:1838	arg1	applications					1840:1851	The main applications	1831:1851	The main applications of the systems obtained	1831:1875	The main applications of the systems obtained are in the area of drug release for tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example).
25175227	5	71	from	inclusion	895:903	arg1	vesicles					960:967	multilamellar vesicles	946:967	multilamellar vesicles (MLVs)	946:974	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	5	71	from	inclusion	895:903	arg1	MLVs					970:973	MLVs	970:973	MLVs	970:973	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	5	71	from	inclusion	895:903	arg1	SUVs					936:939	SUVs	936:939	SUVs	936:939	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	5	71	from	inclusion	895:903	arg1	vesicles					926:933	small unilamellar vesicles	908:933	small unilamellar vesicles (SUVs)	908:940	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	1	72	theme	hydrogels	130:138	arg1	preparation					98:108	the preparation	94:108	the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine	94:339	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	1	72	theme	hydrogels	130:138	arg1	matrices					257:264	matrices	257:264	matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine	257:339	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	12	73	theme	risk	2301:2304	arg1	cancer					2325:2330	cancer	2325:2330	cancer	2325:2330	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	12	73	theme	risk	2301:2304	arg1	diseases					2306:2313	many high risk diseases	2291:2313	many high risk diseases including cancer	2291:2330	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	9	74	theme	unilamellarity	1726:1739	arg1	instability					1749:1759	their unilamellarity related instability	1720:1759	their unilamellarity related instability even inside the hydrogel	1720:1784	When small unilamellar liposomes were tested, calcein have been released from hydrogels predominantly in a free form (due to their unilamellarity related instability even inside the hydrogel) but in a sustained and controllable manner.
25175227	6	75	theme	systems	1079:1085	arg1	kinetics					1059:1066	The calcein release kinetics	1039:1066	The calcein release kinetics of complex systems	1039:1085	The calcein release kinetics of complex systems were compared to simple systems (control hydrogels) and important changes were observed thus proving that the mechanism of the process increases in complexity.
25175227	12	76	theme	tissue	2241:2246	arg1	hydrogels					2214:2222	These hydrogels	2208:2222	These hydrogels	2208:2222	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	12	76	theme	tissue	2241:2246	arg1	replacement					2248:2258	tissue replacement	2241:2258	tissue replacement	2241:2258	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	10	77	theme	systems	1860:1866	arg1	applications					1840:1851	The main applications	1831:1851	The main applications of the systems obtained	1831:1875	The main applications of the systems obtained are in the area of drug release for tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example).
25175227	1	78	theme	double	153:158	arg1	crosslinking					160:171	double crosslinking	153:171	double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate	153:235	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	0	79	from	liposomes	23:31	arg1	release					10:16	Modulated release	0:16	Modulated release from liposomes	0:31	Modulated release from liposomes entrapped in chitosan/gelatin hydrogels.
25175227	12	80	from	systems	2280:2286	arg1	cancer					2325:2330	cancer	2325:2330	cancer	2325:2330	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	12	80	from	systems	2280:2286	arg1	diseases					2306:2313	many high risk diseases	2291:2313	many high risk diseases including cancer	2291:2330	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	6	81	theme	release	1051:1057	arg1	kinetics					1059:1066	The calcein release kinetics	1039:1066	The calcein release kinetics of complex systems	1039:1085	The calcein release kinetics of complex systems were compared to simple systems (control hydrogels) and important changes were observed thus proving that the mechanism of the process increases in complexity.
25175227	9	82	theme	sustained	1796:1804	arg1	manner					1823:1828	a sustained and controllable manner	1794:1828	a sustained and controllable manner	1794:1828	When small unilamellar liposomes were tested, calcein have been released from hydrogels predominantly in a free form (due to their unilamellarity related instability even inside the hydrogel) but in a sustained and controllable manner.
25175227	9	83	theme	controllable	1810:1821	arg1	manner					1823:1828	a sustained and controllable manner	1794:1828	a sustained and controllable manner	1794:1828	When small unilamellar liposomes were tested, calcein have been released from hydrogels predominantly in a free form (due to their unilamellarity related instability even inside the hydrogel) but in a sustained and controllable manner.
25175227	12	84	theme	depot	2274:2278	arg1	systems					2280:2286	injectable depot systems	2263:2286	injectable depot systems	2263:2286	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	12	84	theme	depot	2274:2278	arg1	hydrogels					2214:2222	These hydrogels	2208:2222	These hydrogels	2208:2222	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	8	85	from	matrix	1522:1527	arg1	release					1505:1511	release	1505:1511	release from the matrix	1505:1527	Multilamellar liposomes showed a better release behaviour, which indicates that these calcein loaded vesicles remained intact to some extent after release from the matrix, due to their improved stability provided by the multiple layers.
25175227	5	86	theme	variable	1017:1024	arg1	composition					1026:1036	variable composition	1017:1036	variable composition	1017:1036	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	8	87	theme	multiple	1578:1585	arg1	layers					1587:1592	the multiple layers	1574:1592	the multiple layers	1574:1592	Multilamellar liposomes showed a better release behaviour, which indicates that these calcein loaded vesicles remained intact to some extent after release from the matrix, due to their improved stability provided by the multiple layers.
25175227	8	88	theme	release	1398:1404	arg1	behaviour					1406:1414	a better release behaviour	1389:1414	a better release behaviour	1389:1414	Multilamellar liposomes showed a better release behaviour, which indicates that these calcein loaded vesicles remained intact to some extent after release from the matrix, due to their improved stability provided by the multiple layers.
25175227	5	89	theme	hydrophilic	785:795	arg1	drug					797:800	a model hydrophilic drug	777:800	a model hydrophilic drug	777:800	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	9	90	theme	unilamellar	1606:1616	arg1	liposomes					1618:1626	small unilamellar liposomes	1600:1626	small unilamellar liposomes	1600:1626	When small unilamellar liposomes were tested, calcein have been released from hydrogels predominantly in a free form (due to their unilamellarity related instability even inside the hydrogel) but in a sustained and controllable manner.
25175227	12	91	theme	injectable	2263:2272	arg1	systems					2280:2286	injectable depot systems	2263:2286	injectable depot systems	2263:2286	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	12	91	theme	injectable	2263:2272	arg1	hydrogels					2214:2222	These hydrogels	2208:2222	These hydrogels	2208:2222	These hydrogels could be used as tissue replacement or injectable depot systems in many high risk diseases including cancer.
25175227	1	92	dep	glutaraldehyde	178:191	arg1	tripolyphosphate					220:235	sulphate/sodium tripolyphosphate	204:235	sulphate/sodium tripolyphosphate	204:235	The paper describes the preparation of chitosan/gelatin hydrogels, obtained by double crosslinking with glutaraldehyde and sodium sulphate/sodium tripolyphosphate that may be used as matrices for the inclusion of drug loaded liposomes composed of phosphatidylcholine.
25175227	5	93	theme	several	859:865	arg1	days					867:870	several days	859:870	several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition	859:1036	The present study shows that calcein (used as a model hydrophilic drug) release from polymeric hydrogels has been retarded from several days to weeks after calcein inclusion in small unilamellar vesicles (SUVs) and multilamellar vesicles (MLVs) entrapped subsequently in hydrogels with variable composition.
25175227	6	94	theme	simple	1104:1109	arg1	systems					1111:1117	simple systems	1104:1117	simple systems (control hydrogels)	1104:1137	The calcein release kinetics of complex systems were compared to simple systems (control hydrogels) and important changes were observed thus proving that the mechanism of the process increases in complexity.
25175227	6	94	theme	simple	1104:1109	arg1	hydrogels					1128:1136	control hydrogels	1120:1136	control hydrogels	1120:1136	The calcein release kinetics of complex systems were compared to simple systems (control hydrogels) and important changes were observed thus proving that the mechanism of the process increases in complexity.
25175227	8	95	dep	showed	1382:1387	arg1	indicates					1423:1431	indicates	1423:1431	indicates that these calcein loaded vesicles remained intact to some extent after release from the matrix, due to their improved stability provided by the multiple layers	1423:1592	Multilamellar liposomes showed a better release behaviour, which indicates that these calcein loaded vesicles remained intact to some extent after release from the matrix, due to their improved stability provided by the multiple layers.
25175227	10	96	dep	repair	1939:1944	arg1	administration					1955:1968	topical administration	1947:1968	topical administration of drugs for wound therapy - burns, for example	1947:2016	The main applications of the systems obtained are in the area of drug release for tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example).
25175227	11	97	theme	drugs	2201:2205	arg1	term					2170:2173	long term	2165:2173	long term	2165:2173	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	11	97	theme	drugs	2201:2205	arg1	delivery					2189:2196	sustained delivery	2179:2196	sustained delivery	2179:2196	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
25175227	10	98	theme	tissue	1913:1918	arg1	repair					1939:1944	tissue engineering/tissue repair	1913:1944	tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example)	1913:2017	The main applications of the systems obtained are in the area of drug release for tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example).
25175227	8	99	theme	loaded	1452:1457	arg1	vesicles					1459:1466	these calcein loaded vesicles	1438:1466	these calcein loaded vesicles	1438:1466	Multilamellar liposomes showed a better release behaviour, which indicates that these calcein loaded vesicles remained intact to some extent after release from the matrix, due to their improved stability provided by the multiple layers.
25175227	10	100	theme	release	1901:1907	arg1	area					1888:1891	the area	1884:1891	the area of drug release	1884:1907	The main applications of the systems obtained are in the area of drug release for tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example).
25175227	4	101	theme	ionic	675:679	arg1	crosslinker					681:691	ionic crosslinker	675:691	ionic crosslinker	675:691	Samples consisting of different chitosan/gelatin ratios and type/amount of ionic crosslinker have been prepared and characterized.
25175227	10	102	theme	topical	1947:1953	arg1	administration					1955:1968	topical administration	1947:1968	topical administration of drugs for wound therapy - burns, for example	1947:2016	The main applications of the systems obtained are in the area of drug release for tissue engineering/tissue repair (topical administration of drugs for wound therapy - burns, for example).
25175227	11	103	theme	sustained	2179:2187	arg1	delivery					2189:2196	sustained delivery	2179:2196	sustained delivery	2179:2196	Hydrogels capable of delivering drugs over prolonged periods of time represent a step forward in wound management and many diseases that request long term and sustained delivery of drugs.
27987511	7	0	from	shape	911:915	arg1	micron-sized					870:881	micron-sized	870:881	micron-sized	870:881	The microspheres were micron-sized (5.982 μm) and spherical in shape with smooth surface morphology.
27987511	7	0	from	shape	911:915	arg1	μm					890:891	5.982 μm	884:891	5.982 μm	884:891	The microspheres were micron-sized (5.982 μm) and spherical in shape with smooth surface morphology.
27987511	7	0	from	shape	911:915	arg1	microspheres					852:863	The microspheres	848:863	The microspheres	848:863	The microspheres were micron-sized (5.982 μm) and spherical in shape with smooth surface morphology.
27987511	10	1	dep	in	1133:1134	arg1	vitro					1136:1140	vitro	1136:1140	vitro	1136:1140	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	5	2	theme	immersion	669:677	arg1	method					679:684	The dynamic immersion method	657:684	The dynamic immersion method	657:684	The dynamic immersion method was used to examine the in vitro release characteristic of BMP-2.
27987511	5	3	theme	in	710:711	arg1	characteristic					727:740	the in vitro release characteristic	706:740	the in vitro release characteristic of BMP-2	706:749	The dynamic immersion method was used to examine the in vitro release characteristic of BMP-2.
27987511	11	4	theme	delivery	1295:1302	arg1	system					1304:1309	The drug delivery system	1286:1309	The drug delivery system	1286:1309	CONCLUSION The drug delivery system achieves the biological function of BMP-2 and sustaining slow release in bone repair parts.
27987511	7	5	theme	smooth	922:927	arg1	morphology					937:946	smooth surface morphology	922:946	smooth surface morphology	922:946	The microspheres were micron-sized (5.982 μm) and spherical in shape with smooth surface morphology.
27987511	4	6	theme	loading	597:603	arg1	capacity					605:612	loading capacity	597:612	loading capacity	597:612	Characterization preserved the loading capacity and encapsulation efficiency of the BMP-2.
27987511	0	7	theme	composite	86:94	arg1	scaffold					96:103	bovine bone composite scaffold	74:103	bovine bone composite scaffold	74:103	[Synthesis and in vitro characterization of chitosan microspheres/ceramic bovine bone composite scaffold].
27987511	11	8	theme	slow	1368:1371	arg1	release					1373:1379	slow release	1368:1379	slow release in bone repair parts	1368:1400	CONCLUSION The drug delivery system achieves the biological function of BMP-2 and sustaining slow release in bone repair parts.
27987511	8	9	theme	mg	999:1000	arg1	chitosan/BMP-2					1002:1015	5 mg chitosan/BMP-2	997:1015	5 mg chitosan/BMP-2 soaked for 21 days with a gradual release of BMP-2	997:1066	From the release experiments, it was found that 5 mg chitosan/BMP-2 soaked for 21 days with a gradual release of BMP-2.
27987511	2	10	theme	delivery	346:353	arg1	system					355:360	the drug delivery system	337:360	the drug delivery system	337:360	Then the chitosan microspheres were loaded in the ceramic bovine bone (CBB) to achieve the drug delivery system.
27987511	8	11	theme	gradual	1043:1049	arg1	release					1051:1057	a gradual release	1041:1057	a gradual release of BMP-2	1041:1066	From the release experiments, it was found that 5 mg chitosan/BMP-2 soaked for 21 days with a gradual release of BMP-2.
27987511	5	12	used	used	690:693	arg2	method					679:684	The dynamic immersion method	657:684	The dynamic immersion method	657:684	The dynamic immersion method was used to examine the in vitro release characteristic of BMP-2.
27987511	3	13	theme	chemical	375:382	arg1	structure					384:392	chemical structure	375:392	chemical structure	375:392	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	3	13	theme	chemical	375:382	arg1	METHODS					363:369	METHODS The chemical structure and surface morphology	363:415	METHODS The chemical structure and surface morphology of the drug delivery system	363:443	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	3	14	dep	structure	384:392	arg1	The					371:373	The	371:373	The	371:373	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	8	15	theme	5	997:997	arg1	mg					999:1000	mg	999:1000	mg	999:1000	From the release experiments, it was found that 5 mg chitosan/BMP-2 soaked for 21 days with a gradual release of BMP-2.
27987511	2	16	theme	bovine	308:313	arg1	CBB					321:323	CBB	321:323	CBB	321:323	Then the chitosan microspheres were loaded in the ceramic bovine bone (CBB) to achieve the drug delivery system.
27987511	2	16	theme	bovine	308:313	arg1	bone					315:318	the ceramic bovine bone	296:318	the ceramic bovine bone (CBB)	296:324	Then the chitosan microspheres were loaded in the ceramic bovine bone (CBB) to achieve the drug delivery system.
27987511	10	17	theme	osteogenic	1247:1256	arg1	differentiation					1258:1272	osteogenic differentiation	1247:1272	osteogenic differentiation	1247:1272	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	10	17	theme	osteogenic	1247:1256	arg1	cells					1223:1227	MC3T3-E1 cells proliferation and osteogenic differentiation	1214:1272	MC3T3-E1 cells proliferation and osteogenic differentiation	1214:1272	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	7	18	with	micron-sized	870:881	arg1	morphology					937:946	smooth surface morphology	922:946	smooth surface morphology	922:946	The microspheres were micron-sized (5.982 μm) and spherical in shape with smooth surface morphology.
27987511	5	19	dep	in	710:711	arg1	vitro					713:717	vitro	713:717	vitro	713:717	The dynamic immersion method was used to examine the in vitro release characteristic of BMP-2.
27987511	3	20	dep	spectroscopy	493:504	arg1	observation					553:563	observation	553:563	observation	553:563	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	2	21	theme	ceramic	300:306	arg1	CBB					321:323	CBB	321:323	CBB	321:323	Then the chitosan microspheres were loaded in the ceramic bovine bone (CBB) to achieve the drug delivery system.
27987511	2	21	theme	ceramic	300:306	arg1	bone					315:318	the ceramic bovine bone	296:318	the ceramic bovine bone (CBB)	296:324	Then the chitosan microspheres were loaded in the ceramic bovine bone (CBB) to achieve the drug delivery system.
27987511	10	22	theme	delivery	1181:1188	arg1	system					1190:1195	this novel drug delivery system	1165:1195	this novel drug delivery system	1165:1195	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	1	23	theme	chitosan	121:128	arg1	microspheres					130:141	The chitosan microspheres	117:141	The chitosan microspheres encapsulated with bone morphogenetic protein-2 (BMP-2)	117:196	OBJECTIVE The chitosan microspheres encapsulated with bone morphogenetic protein-2 (BMP-2) were prepared by the emulsion cross-linking method.
27987511	11	24	theme	biological	1324:1333	arg1	function					1335:1342	the biological function	1320:1342	the biological function of BMP-2	1320:1351	CONCLUSION The drug delivery system achieves the biological function of BMP-2 and sustaining slow release in bone repair parts.
27987511	4	25	theme	BMP-2	650:654	arg1	capacity					605:612	loading capacity	597:612	loading capacity	597:612	Characterization preserved the loading capacity and encapsulation efficiency of the BMP-2.
27987511	4	25	theme	BMP-2	650:654	arg1	efficiency					632:641	encapsulation efficiency	618:641	encapsulation efficiency	618:641	Characterization preserved the loading capacity and encapsulation efficiency of the BMP-2.
27987511	11	26	from	release	1373:1379	arg1	parts					1396:1400	bone repair parts	1384:1400	bone repair parts	1384:1400	CONCLUSION The drug delivery system achieves the biological function of BMP-2 and sustaining slow release in bone repair parts.
27987511	9	27	theme	BMP-2	1090:1094	arg1	mg/L					1113:1116	(239.1±20.0) mg/L	1100:1116	(239.1±20.0) mg/L	1100:1116	The concentration of BMP-2 was (239.1±20.0) mg/L on Day 21.
27987511	9	27	theme	BMP-2	1090:1094	arg1	concentration					1073:1085	The concentration	1069:1085	The concentration of BMP-2	1069:1094	The concentration of BMP-2 was (239.1±20.0) mg/L on Day 21.
27987511	3	28	theme	system	438:443	arg1	structure					384:392	chemical structure	375:392	chemical structure	375:392	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	3	28	theme	system	438:443	arg1	morphology					406:415	surface morphology	398:415	surface morphology	398:415	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	3	28	theme	system	438:443	arg1	METHODS					363:369	METHODS The chemical structure and surface morphology	363:415	METHODS The chemical structure and surface morphology of the drug delivery system	363:443	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	11	29	dep	CONCLUSION	1275:1284	arg1	sustaining					1357:1366	sustaining	1357:1366	sustaining slow release in bone repair parts	1357:1400	CONCLUSION The drug delivery system achieves the biological function of BMP-2 and sustaining slow release in bone repair parts.
27987511	11	29	dep	CONCLUSION	1275:1284	arg1	achieves					1311:1318	achieves	1311:1318	achieves the biological function of BMP-2	1311:1351	CONCLUSION The drug delivery system achieves the biological function of BMP-2 and sustaining slow release in bone repair parts.
27987511	8	30	theme	BMP-2	1062:1066	arg1	release					1051:1057	a gradual release	1041:1057	a gradual release of BMP-2	1041:1066	From the release experiments, it was found that 5 mg chitosan/BMP-2 soaked for 21 days with a gradual release of BMP-2.
27987511	10	31	dep	cells	1223:1227	arg1	proliferation					1229:1241	proliferation	1229:1241	proliferation	1229:1241	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	10	31	dep	cells	1223:1227	arg1	differentiation					1258:1272	osteogenic differentiation	1247:1272	osteogenic differentiation	1247:1272	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	10	31	dep	cells	1223:1227	arg1	cells					1223:1227	MC3T3-E1 cells proliferation and osteogenic differentiation	1214:1272	MC3T3-E1 cells proliferation and osteogenic differentiation	1214:1272	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	10	32	theme	novel	1170:1174	arg1	system					1190:1195	this novel drug delivery system	1165:1195	this novel drug delivery system	1165:1195	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	12	33	theme	bone	1497:1500	arg1	scaffolds					1521:1529	bone tissue engineering scaffolds	1497:1529	bone tissue engineering scaffolds	1497:1529	Also it can provide the basis for repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds.
27987511	3	34	theme	drug	424:427	arg1	system					438:443	the drug delivery system	420:443	the drug delivery system	420:443	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	12	35	theme	treatment	1466:1474	arg1	repair					1437:1442	repair	1437:1442	repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds	1437:1529	Also it can provide the basis for repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds.
27987511	5	36	theme	dynamic	661:667	arg1	method					679:684	The dynamic immersion method	657:684	The dynamic immersion method	657:684	The dynamic immersion method was used to examine the in vitro release characteristic of BMP-2.
27987511	12	37	theme	bone	1447:1450	arg1	treatment					1466:1474	bone tissue defect treatment	1447:1474	bone tissue defect treatment	1447:1474	Also it can provide the basis for repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds.
27987511	3	38	theme	delivery	429:436	arg1	system					438:443	the drug delivery system	420:443	the drug delivery system	420:443	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	11	39	theme	BMP-2	1347:1351	arg1	function					1335:1342	the biological function	1320:1342	the biological function of BMP-2	1320:1351	CONCLUSION The drug delivery system achieves the biological function of BMP-2 and sustaining slow release in bone repair parts.
27987511	0	40	theme	in	15:16	arg1	characterization					24:39	in vitro characterization	15:39	in vitro characterization of chitosan	15:51	[Synthesis and in vitro characterization of chitosan microspheres/ceramic bovine bone composite scaffold].
27987511	5	41	theme	release	719:725	arg1	characteristic					727:740	the in vitro release characteristic	706:740	the in vitro release characteristic of BMP-2	706:749	The dynamic immersion method was used to examine the in vitro release characteristic of BMP-2.
27987511	12	42	theme	tissue	1502:1507	arg1	scaffolds					1521:1529	bone tissue engineering scaffolds	1497:1529	bone tissue engineering scaffolds	1497:1529	Also it can provide the basis for repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds.
27987511	11	43	theme	drug	1290:1293	arg1	system					1304:1309	The drug delivery system	1286:1309	The drug delivery system	1286:1309	CONCLUSION The drug delivery system achieves the biological function of BMP-2 and sustaining slow release in bone repair parts.
27987511	3	44	theme	scanning	518:525	arg1	SEM					548:550	SEM	548:550	SEM	548:550	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	3	44	theme	scanning	518:525	arg1	microscope					536:545	scanning electron microscope	518:545	scanning electron microscope (SEM)	518:551	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	4	45	dep	capacity	605:612	arg1	the					593:595	the	593:595	the	593:595	Characterization preserved the loading capacity and encapsulation efficiency of the BMP-2.
27987511	9	46	from	mg/L	1113:1116	arg1	Day					1121:1123	Day 21	1121:1126	Day 21	1121:1126	The concentration of BMP-2 was (239.1±20.0) mg/L on Day 21.
27987511	10	47	theme	MC3T3-E1	1214:1221	arg1	proliferation					1229:1241	proliferation	1229:1241	proliferation	1229:1241	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	10	47	theme	MC3T3-E1	1214:1221	arg1	differentiation					1258:1272	osteogenic differentiation	1247:1272	osteogenic differentiation	1247:1272	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	10	47	theme	MC3T3-E1	1214:1221	arg1	cells					1223:1227	MC3T3-E1 cells proliferation and osteogenic differentiation	1214:1272	MC3T3-E1 cells proliferation and osteogenic differentiation	1214:1272	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	1	48	theme	emulsion	219:226	arg1	method					242:247	the emulsion cross-linking method	215:247	the emulsion cross-linking method	215:247	OBJECTIVE The chitosan microspheres encapsulated with bone morphogenetic protein-2 (BMP-2) were prepared by the emulsion cross-linking method.
27987511	11	49	theme	bone	1384:1387	arg1	parts					1396:1400	bone repair parts	1384:1400	bone repair parts	1384:1400	CONCLUSION The drug delivery system achieves the biological function of BMP-2 and sustaining slow release in bone repair parts.
27987511	11	50	theme	repair	1389:1394	arg1	parts					1396:1400	bone repair parts	1384:1400	bone repair parts	1384:1400	CONCLUSION The drug delivery system achieves the biological function of BMP-2 and sustaining slow release in bone repair parts.
27987511	12	51	theme	scaffolds	1521:1529	arg1	selection					1484:1492	the selection	1480:1492	the selection of bone tissue engineering scaffolds	1480:1529	Also it can provide the basis for repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds.
27987511	12	51	theme	scaffolds	1521:1529	arg1	treatment					1466:1474	bone tissue defect treatment	1447:1474	bone tissue defect treatment	1447:1474	Also it can provide the basis for repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds.
27987511	7	52	from	micron-sized	870:881	arg1	shape					911:915	shape	911:915	shape	911:915	The microspheres were micron-sized (5.982 μm) and spherical in shape with smooth surface morphology.
27987511	8	53	theme	release	958:964	arg1	experiments					966:976	the release experiments	954:976	the release experiments	954:976	From the release experiments, it was found that 5 mg chitosan/BMP-2 soaked for 21 days with a gradual release of BMP-2.
27987511	1	54	theme	cross-linking	228:240	arg1	method					242:247	the emulsion cross-linking method	215:247	the emulsion cross-linking method	215:247	OBJECTIVE The chitosan microspheres encapsulated with bone morphogenetic protein-2 (BMP-2) were prepared by the emulsion cross-linking method.
27987511	3	55	theme	surface	398:404	arg1	morphology					406:415	surface morphology	398:415	surface morphology	398:415	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	3	55	theme	surface	398:404	arg1	METHODS					363:369	METHODS The chemical structure and surface morphology	363:415	METHODS The chemical structure and surface morphology of the drug delivery system	363:443	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	5	56	theme	BMP-2	745:749	arg1	characteristic					727:740	the in vitro release characteristic	706:740	the in vitro release characteristic of BMP-2	706:749	The dynamic immersion method was used to examine the in vitro release characteristic of BMP-2.
27987511	12	57	theme	engineering	1509:1519	arg1	scaffolds					1521:1529	bone tissue engineering scaffolds	1497:1529	bone tissue engineering scaffolds	1497:1529	Also it can provide the basis for repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds.
27987511	2	58	theme	drug	341:344	arg1	system					355:360	the drug delivery system	337:360	the drug delivery system	337:360	Then the chitosan microspheres were loaded in the ceramic bovine bone (CBB) to achieve the drug delivery system.
27987511	0	59	theme	chitosan	44:51	arg1	[Synthesis					0:9	[Synthesis	0:9	[Synthesis	0:9	[Synthesis and in vitro characterization of chitosan microspheres/ceramic bovine bone composite scaffold].
27987511	0	59	theme	chitosan	44:51	arg1	characterization					24:39	in vitro characterization	15:39	in vitro characterization of chitosan	15:51	[Synthesis and in vitro characterization of chitosan microspheres/ceramic bovine bone composite scaffold].
27987511	10	60	theme	in	1133:1134	arg1	experiment					1142:1151	The in vitro experiment	1129:1151	The in vitro experiment	1129:1151	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	3	61	dep	transform	474:482	arg1	infrared					484:491	infrared	484:491	transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation	474:563	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	1	62	theme	bone	161:164	arg1	BMP-2					191:195	BMP-2	191:195	BMP-2	191:195	OBJECTIVE The chitosan microspheres encapsulated with bone morphogenetic protein-2 (BMP-2) were prepared by the emulsion cross-linking method.
27987511	1	62	theme	bone	161:164	arg1	protein-2					180:188	bone morphogenetic protein-2	161:188	bone morphogenetic protein-2 (BMP-2)	161:196	OBJECTIVE The chitosan microspheres encapsulated with bone morphogenetic protein-2 (BMP-2) were prepared by the emulsion cross-linking method.
27987511	6	63	theme	chitosan	764:771	arg1	microspheres					773:784	The chitosan microspheres	760:784	The chitosan microspheres	760:784	RESULTS The chitosan microspheres were successfully encapsulated BMP-2 by cross-linking method.
27987511	12	64	theme	defect	1459:1464	arg1	treatment					1466:1474	bone tissue defect treatment	1447:1474	bone tissue defect treatment	1447:1474	Also it can provide the basis for repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds.
27987511	10	65	theme	drug	1176:1179	arg1	system					1190:1195	this novel drug delivery system	1165:1195	this novel drug delivery system	1165:1195	The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
27987511	3	66	dep	METHODS	363:369	arg1	structure					384:392	chemical structure	375:392	chemical structure	375:392	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	3	66	dep	METHODS	363:369	arg1	morphology					406:415	surface morphology	398:415	surface morphology	398:415	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	3	66	dep	METHODS	363:369	arg1	METHODS					363:369	METHODS The chemical structure and surface morphology	363:415	METHODS The chemical structure and surface morphology of the drug delivery system	363:443	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	0	67	dep	in	15:16	arg1	vitro					18:22	vitro	18:22	vitro	18:22	[Synthesis and in vitro characterization of chitosan microspheres/ceramic bovine bone composite scaffold].
27987511	1	68	theme	morphogenetic	166:178	arg1	BMP-2					191:195	BMP-2	191:195	BMP-2	191:195	OBJECTIVE The chitosan microspheres encapsulated with bone morphogenetic protein-2 (BMP-2) were prepared by the emulsion cross-linking method.
27987511	1	68	theme	morphogenetic	166:178	arg1	protein-2					180:188	bone morphogenetic protein-2	161:188	bone morphogenetic protein-2 (BMP-2)	161:196	OBJECTIVE The chitosan microspheres encapsulated with bone morphogenetic protein-2 (BMP-2) were prepared by the emulsion cross-linking method.
27987511	7	69	theme	surface	929:935	arg1	morphology					937:946	smooth surface morphology	922:946	smooth surface morphology	922:946	The microspheres were micron-sized (5.982 μm) and spherical in shape with smooth surface morphology.
27987511	4	70	theme	encapsulation	618:630	arg1	efficiency					632:641	encapsulation efficiency	618:641	encapsulation efficiency	618:641	Characterization preserved the loading capacity and encapsulation efficiency of the BMP-2.
27987511	0	71	theme	bone	81:84	arg1	scaffold					96:103	bovine bone composite scaffold	74:103	bovine bone composite scaffold	74:103	[Synthesis and in vitro characterization of chitosan microspheres/ceramic bovine bone composite scaffold].
27987511	12	72	theme	selection	1484:1492	arg1	repair					1437:1442	repair	1437:1442	repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds	1437:1529	Also it can provide the basis for repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds.
27987511	3	73	dep	Fourier	466:472	arg1	transform					474:482	transform	474:482	transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation	474:563	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	7	74	dep	micron-sized	870:881	arg1	micron-sized					870:881	micron-sized	870:881	micron-sized	870:881	The microspheres were micron-sized (5.982 μm) and spherical in shape with smooth surface morphology.
27987511	7	74	dep	micron-sized	870:881	arg1	μm					890:891	5.982 μm	884:891	5.982 μm	884:891	The microspheres were micron-sized (5.982 μm) and spherical in shape with smooth surface morphology.
27987511	7	74	dep	micron-sized	870:881	arg1	microspheres					852:863	The microspheres	848:863	The microspheres	848:863	The microspheres were micron-sized (5.982 μm) and spherical in shape with smooth surface morphology.
27987511	1	75	dep	OBJECTIVE	107:115	arg1	prepared					203:210	prepared	203:210	were prepared by the emulsion cross-linking method	198:247	OBJECTIVE The chitosan microspheres encapsulated with bone morphogenetic protein-2 (BMP-2) were prepared by the emulsion cross-linking method.
27987511	6	76	dep	RESULTS	752:758	arg1	encapsulated					804:815	encapsulated	804:815	were successfully encapsulated BMP-2 by cross-linking method	786:845	RESULTS The chitosan microspheres were successfully encapsulated BMP-2 by cross-linking method.
27987511	0	77	theme	bovine	74:79	arg1	scaffold					96:103	bovine bone composite scaffold	74:103	bovine bone composite scaffold	74:103	[Synthesis and in vitro characterization of chitosan microspheres/ceramic bovine bone composite scaffold].
27987511	12	78	theme	tissue	1452:1457	arg1	treatment					1466:1474	bone tissue defect treatment	1447:1474	bone tissue defect treatment	1447:1474	Also it can provide the basis for repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds.
27987511	2	79	theme	chitosan	259:266	arg1	microspheres					268:279	the chitosan microspheres	255:279	the chitosan microspheres	255:279	Then the chitosan microspheres were loaded in the ceramic bovine bone (CBB) to achieve the drug delivery system.
27987511	3	80	theme	electron	527:534	arg1	SEM					548:550	SEM	548:550	SEM	548:550	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
27987511	3	80	theme	electron	527:534	arg1	microscope					536:545	scanning electron microscope	518:545	scanning electron microscope (SEM)	518:551	METHODS The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation.
28419693	7	0	theme	expression	1311:1320	arg1	levels					1322:1327	the expression levels	1307:1327	the expression levels of osteogenic-related RUNX2 and BMP-2	1307:1365	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	8	1	from	nanoparticles	1467:1479	arg1	scaffold					1498:1505	the composite scaffold	1484:1505	the composite scaffold	1484:1505	Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus.
28419693	4	2	theme	nm	674:675	arg1	thicknesses					655:665	thicknesses	655:665	thicknesses of ∼50 nm	655:675	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	4	2	theme	nm	674:675	arg1	sizes					631:635	lateral sizes	623:635	lateral sizes of 200-400 nm	623:649	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	7	3	theme	composite	1173:1181	arg1	scaffold					1183:1190	the composite scaffold	1169:1190	the composite scaffold	1169:1190	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	5	4	link	marrow-derived	794:807	arg1	hBMSCs					833:838	hBMSCs	833:838	hBMSCs	833:838	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	4	link	marrow-derived	794:807	arg1	cells					826:830	Human bone marrow-derived mesenchymal stem cells	783:830	Human bone marrow-derived mesenchymal stem cells (hBMSCs)	783:839	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	1	5	theme	large	211:215	arg1	defects					222:228	large bone defects	211:228	large bone defects	211:228	Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection.
28419693	7	6	theme	Sr2+	1154:1157	arg1	ions					1159:1162	The released Sr2+ ions	1141:1162	The released Sr2+ ions from the composite scaffold	1141:1190	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	1	7	theme	defects	222:228	arg1	reconstruction					193:206	the reconstruction	189:206	the reconstruction of large bone defects	189:228	Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection.
28419693	8	8	contain	possesses	1414:1422	arg1	scaffold					1405:1412	the Ag-MgSrFe/CS composite scaffold	1378:1412	the Ag-MgSrFe/CS composite scaffold	1378:1412	Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus.
28419693	8	8	contain	possesses	1414:1422	arg2	property					1443:1450	good antibacterial property	1424:1450	good antibacterial property	1424:1450	Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus.
28419693	9	9	theme	Ag-MgSrFe/CS	1575:1586	arg1	scaffold					1598:1605	the Ag-MgSrFe/CS composite scaffold	1571:1605	the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property	1571:1664	Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering.
28419693	9	10	theme	excellent	1612:1620	arg1	osteoinductivity					1622:1637	excellent osteoinductivity	1612:1637	excellent osteoinductivity	1612:1637	Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering.
28419693	5	11	theme	Human	783:787	arg1	hBMSCs					833:838	hBMSCs	833:838	hBMSCs	833:838	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	11	theme	Human	783:787	arg1	cells					826:830	Human bone marrow-derived mesenchymal stem cells	783:830	Human bone marrow-derived mesenchymal stem cells (hBMSCs)	783:839	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	12	theme	composite	913:921	arg1	scaffold					923:930	the Ag-MgSrFe/CS composite scaffold	896:930	the Ag-MgSrFe/CS composite scaffold	896:930	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	1	13	theme	Bone	151:154	arg1	scaffolds					175:183	Bone tissue engineering scaffolds	151:183	Bone tissue engineering scaffolds for the reconstruction of large bone defects	151:228	Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection.
28419693	8	14	theme	Ag-MgSrFe/CS	1382:1393	arg1	scaffold					1405:1412	the Ag-MgSrFe/CS composite scaffold	1378:1412	the Ag-MgSrFe/CS composite scaffold	1378:1412	Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus.
28419693	1	15	theme	engineering	163:173	arg1	scaffolds					175:183	Bone tissue engineering scaffolds	151:183	Bone tissue engineering scaffolds for the reconstruction of large bone defects	151:228	Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection.
28419693	0	16	theme	osteogenic	84:93	arg1	property					113:120	enhanced osteogenic and antibacterial property	75:120	enhanced osteogenic and antibacterial property for bone engineering tissue	75:148	Ag-loaded MgSrFe-layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue.
28419693	7	17	theme	hBMSCs	1237:1242	arg1	activity					1225:1232	the alkaline phosphatase activity	1200:1232	the alkaline phosphatase activity of hBMSCs	1200:1242	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	5	18	theme	Ag	953:954	arg1	elements					963:970	the Ag and Sr elements	949:970	the Ag and Sr elements in the composite scaffold	949:996	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	4	19	theme	hydroxide	577:585	arg1	nanoplates					587:596	The layered double hydroxide nanoplates	558:596	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS	558:616	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	8	20	theme	good	1424:1427	arg1	property					1443:1450	good antibacterial property	1424:1450	good antibacterial property	1424:1450	Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus.
28419693	0	21	theme	antibacterial	99:111	arg1	property					113:120	enhanced osteogenic and antibacterial property	75:120	enhanced osteogenic and antibacterial property for bone engineering tissue	75:148	Ag-loaded MgSrFe-layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue.
28419693	5	22	theme	Sr	960:961	arg1	elements					963:970	the Ag and Sr elements	949:970	the Ag and Sr elements in the composite scaffold	949:996	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	6	23	theme	MgFe/CS	1045:1051	arg1	scaffold					1063:1070	MgFe/CS composite scaffold	1045:1070	MgFe/CS composite scaffold	1045:1070	When compared with MgFe/CS composite scaffold, the Ag-MgSrFe/CS composite scaffold has better osteogenic property.
28419693	5	24	theme	marrow-derived	794:807	arg1	hBMSCs					833:838	hBMSCs	833:838	hBMSCs	833:838	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	24	theme	marrow-derived	794:807	arg1	cells					826:830	Human bone marrow-derived mesenchymal stem cells	783:830	Human bone marrow-derived mesenchymal stem cells (hBMSCs)	783:839	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	25	theme	composite	979:987	arg1	scaffold					989:996	the composite scaffold	975:996	the composite scaffold	975:996	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	26	theme	stem	821:824	arg1	hBMSCs					833:838	hBMSCs	833:838	hBMSCs	833:838	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	26	theme	stem	821:824	arg1	cells					826:830	Human bone marrow-derived mesenchymal stem cells	783:830	Human bone marrow-derived mesenchymal stem cells (hBMSCs)	783:839	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	9	27	theme	bone	1692:1695	arg1	engineering					1704:1714	bone tissue engineering	1692:1714	bone tissue engineering	1692:1714	Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering.
28419693	4	28	theme	layered	562:568	arg1	nanoplates					587:596	The layered double hydroxide nanoplates	558:596	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS	558:616	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	11	29	dep	Res	1763:1765	arg1	B					1772:1772	Part B	1767:1772	J Biomed Mater Res Part B: Appl Biomater, 106B: 863-873, 2018.	1748:1809	J Biomed Mater Res Part B: Appl Biomater, 106B: 863-873, 2018.
28419693	11	29	dep	Res	1763:1765	arg1	Biomater					1780:1787	Biomater	1780:1787	Biomater	1780:1787	J Biomed Mater Res Part B: Appl Biomater, 106B: 863-873, 2018.
28419693	11	29	dep	Res	1763:1765	arg1	2018					1805:1808	2018	1805:1808	2018	1805:1808	J Biomed Mater Res Part B: Appl Biomater, 106B: 863-873, 2018.
28419693	11	29	dep	Res	1763:1765	arg1	106B					1790:1793	106B	1790:1793	106B	1790:1793	J Biomed Mater Res Part B: Appl Biomater, 106B: 863-873, 2018.
28419693	5	30	from	elements	963:970	arg1	scaffold					989:996	the composite scaffold	975:996	the composite scaffold	975:996	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	31	theme	bone	789:792	arg1	hBMSCs					833:838	hBMSCs	833:838	hBMSCs	833:838	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	31	theme	bone	789:792	arg1	cells					826:830	Human bone marrow-derived mesenchymal stem cells	783:830	Human bone marrow-derived mesenchymal stem cells (hBMSCs)	783:839	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	6	32	theme	osteogenic	1120:1129	arg1	property					1131:1138	better osteogenic property	1113:1138	better osteogenic property	1113:1138	When compared with MgFe/CS composite scaffold, the Ag-MgSrFe/CS composite scaffold has better osteogenic property.
28419693	0	33	theme	double	25:30	arg1	scaffold					61:68	MgSrFe-layered double hydroxide/chitosan composite scaffold	10:68	MgSrFe-layered double hydroxide/chitosan composite scaffold	10:68	Ag-loaded MgSrFe-layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue.
28419693	3	34	theme	interconnected	489:502	arg1	structure					516:524	three-dimensional interconnected macroporous structure	471:524	three-dimensional interconnected macroporous structure	471:524	This scaffold exhibits three-dimensional interconnected macroporous structure with a pore size of 100-300 μm.
28419693	4	35	theme	nm	648:649	arg1	thicknesses					655:665	thicknesses	655:665	thicknesses of ∼50 nm	655:675	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	4	35	theme	nm	648:649	arg1	sizes					631:635	lateral sizes	623:635	lateral sizes of 200-400 nm	623:649	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	5	36	theme	good	849:852	arg1	adhesion					854:861	good adhesion	849:861	good adhesion	849:861	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	0	37	theme	composite	51:59	arg1	scaffold					61:68	MgSrFe-layered double hydroxide/chitosan composite scaffold	10:68	MgSrFe-layered double hydroxide/chitosan composite scaffold	10:68	Ag-loaded MgSrFe-layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue.
28419693	6	38	theme	composite	1090:1098	arg1	scaffold					1100:1107	the Ag-MgSrFe/CS composite scaffold	1073:1107	the Ag-MgSrFe/CS composite scaffold	1073:1107	When compared with MgFe/CS composite scaffold, the Ag-MgSrFe/CS composite scaffold has better osteogenic property.
28419693	2	39	theme	hydroxide/chitosan	394:411	arg1	scaffold					438:445	Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold	362:445	Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold	362:445	Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold.
28419693	7	40	theme	alkaline	1204:1211	arg1	phosphatase					1213:1223	the alkaline phosphatase	1200:1223	the alkaline phosphatase activity of hBMSCs	1200:1242	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	4	41	theme	lateral	623:629	arg1	sizes					631:635	lateral sizes	623:635	lateral sizes of 200-400 nm	623:649	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	2	42	theme	MgSrFe-layered	372:385	arg1	Ag-MgSrFe/CS					414:425	Ag-MgSrFe/CS	414:425	Ag-MgSrFe/CS	414:425	Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold.
28419693	2	42	theme	MgSrFe-layered	372:385	arg1	hydroxide/chitosan					394:411	MgSrFe-layered double hydroxide/chitosan	372:411	Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold	362:445	Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold.
28419693	4	43	with	nanoparticles	689:701	arg1	sizes					717:721	particle sizes	708:721	particle sizes of ∼20 nm	708:731	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	2	44	theme	composite	428:436	arg1	scaffold					438:445	Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold	362:445	Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold	362:445	Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold.
28419693	7	45	theme	osteogenic-related	1332:1349	arg1	RUNX2					1351:1355	osteogenic-related RUNX2	1332:1355	osteogenic-related RUNX2	1332:1355	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	11	46	theme	Part	1767:1770	arg1	B					1772:1772	Part B	1767:1772	J Biomed Mater Res Part B: Appl Biomater, 106B: 863-873, 2018.	1748:1809	J Biomed Mater Res Part B: Appl Biomater, 106B: 863-873, 2018.
28419693	4	47	from	nanoplates	587:596	arg1	Ag-MgSrFe/CS					605:616	the Ag-MgSrFe/CS	601:616	the Ag-MgSrFe/CS	601:616	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	7	48	theme	released	1145:1152	arg1	ions					1159:1162	The released Sr2+ ions	1141:1162	The released Sr2+ ions from the composite scaffold	1141:1190	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	5	49	attach	present	841:847	arg2	hBMSCs					833:838	hBMSCs	833:838	hBMSCs	833:838	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	49	attach	present	841:847	arg2	cells					826:830	Human bone marrow-derived mesenchymal stem cells	783:830	Human bone marrow-derived mesenchymal stem cells (hBMSCs)	783:839	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	49	attach	present	841:847	arg1	scaffold					923:930	the Ag-MgSrFe/CS composite scaffold	896:930	the Ag-MgSrFe/CS composite scaffold	896:930	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	1	50	theme	bone	217:220	arg1	defects					222:228	large bone defects	211:228	large bone defects	211:228	Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection.
28419693	4	51	theme	nm	730:731	arg1	sizes					717:721	particle sizes	708:721	particle sizes of ∼20 nm	708:731	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	2	52	theme	first	350:354	arg1	time					356:359	the first time	346:359	the first time	346:359	Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold.
28419693	5	53	contain	have	998:1001	arg2	toxicity					1006:1013	no toxicity	1003:1013	no toxicity to hBMSCs	1003:1023	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	53	contain	have	998:1001	arg1	elements					963:970	the Ag and Sr elements	949:970	the Ag and Sr elements in the composite scaffold	949:996	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	10	54	dep	©	1717:1717	arg1	Inc.					1743:1746	Inc.	1743:1746	Inc.	1743:1746	© 2017 Wiley Periodicals, Inc.
28419693	5	55	theme	Ag-MgSrFe/CS	900:911	arg1	scaffold					923:930	the Ag-MgSrFe/CS composite scaffold	896:930	the Ag-MgSrFe/CS composite scaffold	896:930	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	4	56	theme	particle	708:715	arg1	sizes					717:721	particle sizes	708:721	particle sizes of ∼20 nm	708:731	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	7	57	theme	matrix	1271:1276	arg1	mineralization					1278:1291	the extracellular matrix mineralization	1253:1291	the extracellular matrix mineralization	1253:1291	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	4	58	theme	scaffold	764:771	arg1	surfaces					773:780	the scaffold surfaces	760:780	the scaffold surfaces	760:780	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	1	59	theme	tissue	156:161	arg1	scaffolds					175:183	Bone tissue engineering scaffolds	151:183	Bone tissue engineering scaffolds for the reconstruction of large bone defects	151:228	Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection.
28419693	6	60	theme	Ag-MgSrFe/CS	1077:1088	arg1	scaffold					1100:1107	the Ag-MgSrFe/CS composite scaffold	1073:1107	the Ag-MgSrFe/CS composite scaffold	1073:1107	When compared with MgFe/CS composite scaffold, the Ag-MgSrFe/CS composite scaffold has better osteogenic property.
28419693	9	61	theme	antibacterial	1643:1655	arg1	property					1657:1664	antibacterial property	1643:1664	antibacterial property	1643:1664	Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering.
28419693	8	62	theme	composite	1395:1403	arg1	scaffold					1405:1412	the Ag-MgSrFe/CS composite scaffold	1378:1412	the Ag-MgSrFe/CS composite scaffold	1378:1412	Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus.
28419693	6	63	theme	composite	1053:1061	arg1	scaffold					1063:1070	MgFe/CS composite scaffold	1045:1070	MgFe/CS composite scaffold	1045:1070	When compared with MgFe/CS composite scaffold, the Ag-MgSrFe/CS composite scaffold has better osteogenic property.
28419693	0	64	theme	bone	126:129	arg1	tissue					143:148	bone engineering tissue	126:148	bone engineering tissue	126:148	Ag-loaded MgSrFe-layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue.
28419693	9	65	theme	great	1672:1676	arg1	potential					1678:1686	a great potential	1670:1686	a great potential for bone tissue engineering	1670:1714	Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering.
28419693	7	66	theme	extracellular	1257:1269	arg1	mineralization					1278:1291	the extracellular matrix mineralization	1253:1291	the extracellular matrix mineralization	1253:1291	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	8	67	theme	antibacterial	1429:1441	arg1	property					1443:1450	good antibacterial property	1424:1450	good antibacterial property	1424:1450	Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus.
28419693	5	68	theme	mesenchymal	809:819	arg1	hBMSCs					833:838	hBMSCs	833:838	hBMSCs	833:838	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	5	68	theme	mesenchymal	809:819	arg1	cells					826:830	Human bone marrow-derived mesenchymal stem cells	783:830	Human bone marrow-derived mesenchymal stem cells (hBMSCs)	783:839	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	4	69	theme	double	570:575	arg1	nanoplates					587:596	The layered double hydroxide nanoplates	558:596	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS	558:616	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	3	70	theme	pore	533:536	arg1	size					538:541	a pore size	531:541	a pore size of 100-300 μm	531:555	This scaffold exhibits three-dimensional interconnected macroporous structure with a pore size of 100-300 μm.
28419693	0	71	theme	MgSrFe-layered	10:23	arg1	scaffold					61:68	MgSrFe-layered double hydroxide/chitosan composite scaffold	10:68	MgSrFe-layered double hydroxide/chitosan composite scaffold	10:68	Ag-loaded MgSrFe-layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue.
28419693	9	72	theme	tissue	1697:1702	arg1	engineering					1704:1714	bone tissue engineering	1692:1714	bone tissue engineering	1692:1714	Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering.
28419693	8	73	theme	Ag	1464:1465	arg1	nanoparticles					1467:1479	the Ag nanoparticles	1460:1479	the Ag nanoparticles in the composite scaffold	1460:1505	Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus.
28419693	0	74	theme	hydroxide/chitosan	32:49	arg1	scaffold					61:68	MgSrFe-layered double hydroxide/chitosan composite scaffold	10:68	MgSrFe-layered double hydroxide/chitosan composite scaffold	10:68	Ag-loaded MgSrFe-layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue.
28419693	6	75	theme	better	1113:1118	arg1	property					1131:1138	better osteogenic property	1113:1138	better osteogenic property	1113:1138	When compared with MgFe/CS composite scaffold, the Ag-MgSrFe/CS composite scaffold has better osteogenic property.
28419693	9	76	with	scaffold	1598:1605	arg1	osteoinductivity					1622:1637	excellent osteoinductivity	1612:1637	excellent osteoinductivity	1612:1637	Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering.
28419693	9	76	with	scaffold	1598:1605	arg1	property					1657:1664	antibacterial property	1643:1664	antibacterial property	1643:1664	Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering.
28419693	5	77	dep	Ag	953:954	arg1	the					949:951	the	949:951	the	949:951	Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs.
28419693	3	78	theme	μm	554:555	arg1	size					538:541	a pore size	531:541	a pore size of 100-300 μm	531:555	This scaffold exhibits three-dimensional interconnected macroporous structure with a pore size of 100-300 μm.
28419693	7	79	theme	phosphatase	1213:1223	arg1	activity					1225:1232	the alkaline phosphatase activity	1200:1232	the alkaline phosphatase activity of hBMSCs	1200:1242	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	9	80	theme	composite	1588:1596	arg1	scaffold					1598:1605	the Ag-MgSrFe/CS composite scaffold	1571:1605	the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property	1571:1664	Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering.
28419693	3	81	theme	macroporous	504:514	arg1	structure					516:524	three-dimensional interconnected macroporous structure	471:524	three-dimensional interconnected macroporous structure	471:524	This scaffold exhibits three-dimensional interconnected macroporous structure with a pore size of 100-300 μm.
28419693	8	82	theme	composite	1488:1496	arg1	scaffold					1498:1505	the composite scaffold	1484:1505	the composite scaffold	1484:1505	Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus.
28419693	0	83	theme	engineering	131:141	arg1	tissue					143:148	bone engineering tissue	126:148	bone engineering tissue	126:148	Ag-loaded MgSrFe-layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue.
28419693	0	84	theme	enhanced	75:82	arg1	property					113:120	enhanced osteogenic and antibacterial property	75:120	enhanced osteogenic and antibacterial property for bone engineering tissue	75:148	Ag-loaded MgSrFe-layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue.
28419693	11	85	dep	Biomater	1780:1787	arg1	863-873					1796:1802	863-873	1796:1802	863-873	1796:1802	J Biomed Mater Res Part B: Appl Biomater, 106B: 863-873, 2018.
28419693	6	86	contain	has	1109:1111	arg2	property					1131:1138	better osteogenic property	1113:1138	better osteogenic property	1113:1138	When compared with MgFe/CS composite scaffold, the Ag-MgSrFe/CS composite scaffold has better osteogenic property.
28419693	6	86	contain	has	1109:1111	arg1	scaffold					1100:1107	the Ag-MgSrFe/CS composite scaffold	1073:1107	the Ag-MgSrFe/CS composite scaffold	1073:1107	When compared with MgFe/CS composite scaffold, the Ag-MgSrFe/CS composite scaffold has better osteogenic property.
28419693	7	87	theme	BMP-2	1361:1365	arg1	levels					1322:1327	the expression levels	1307:1327	the expression levels of osteogenic-related RUNX2 and BMP-2	1307:1365	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	3	88	theme	three-dimensional	471:487	arg1	structure					516:524	three-dimensional interconnected macroporous structure	471:524	three-dimensional interconnected macroporous structure	471:524	This scaffold exhibits three-dimensional interconnected macroporous structure with a pore size of 100-300 μm.
28419693	7	89	from	scaffold	1183:1190	arg1	ions					1159:1162	The released Sr2+ ions	1141:1162	The released Sr2+ ions from the composite scaffold	1141:1190	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	9	90	contain	has	1666:1668	arg2	potential					1678:1686	a great potential	1670:1686	a great potential for bone tissue engineering	1670:1714	Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering.
28419693	9	90	contain	has	1666:1668	arg1	scaffold					1598:1605	the Ag-MgSrFe/CS composite scaffold	1571:1605	the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property	1571:1664	Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering.
28419693	2	91	theme	double	387:392	arg1	Ag-MgSrFe/CS					414:425	Ag-MgSrFe/CS	414:425	Ag-MgSrFe/CS	414:425	Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold.
28419693	2	91	theme	double	387:392	arg1	hydroxide/chitosan					394:411	MgSrFe-layered double hydroxide/chitosan	372:411	Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold	362:445	Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold.
28419693	7	92	theme	RUNX2	1351:1355	arg1	levels					1322:1327	the expression levels	1307:1327	the expression levels of osteogenic-related RUNX2 and BMP-2	1307:1365	The released Sr2+ ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2.
28419693	8	93	theme	biofilm	1527:1533	arg1	formation					1535:1543	biofilm formation	1527:1543	biofilm formation	1527:1543	Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus.
28419693	2	94	theme	Ag-loaded	362:370	arg1	scaffold					438:445	Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold	362:445	Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold	362:445	Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold.
28419693	1	95	theme	osteogenic	260:269	arg1	differentiation					271:285	osteogenic differentiation	260:285	osteogenic differentiation	260:285	Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection.
28419693	4	96	theme	Ag	686:687	arg1	nanoparticles					689:701	the Ag nanoparticles	682:701	the Ag nanoparticles with particle sizes of ∼20 nm	682:731	The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces.
28419693	1	97	theme	postoperative	297:309	arg1	infection					311:319	postoperative infection	297:319	postoperative infection	297:319	Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection.
24615298	10	0	theme	cytokine	1663:1670	arg1	release					1672:1678	cytokine release	1663:1678	cytokine release	1663:1678	CLINICAL RELEVANCE Attenuated cytokine release could have implications for the macrophage immune response and result in impaired bacterial clearance.
24615298	9	1	theme	degradation	1584:1594	arg1	products					1596:1603	dental degradation products	1577:1603	dental degradation products	1577:1603	CONCLUSION The present findings emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments.
24615298	3	2	theme	monomer	664:670	arg1	TEGDMA					706:711	TEGDMA	706:711	TEGDMA	706:711	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	2	theme	monomer	664:670	arg1	dimethacrylate					690:703	the methacrylate monomer triethyleneglycol dimethacrylate	647:703	the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA)	647:712	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	1	3	from	fillings	299:306	arg1	monomers					247:254	monomers	247:254	monomers	247:254	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	1	3	from	fillings	299:306	arg1	particles					267:275	filler particles	260:275	filler particles	260:275	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	11	4	theme	biofilms	1863:1870	arg1	formation					1843:1851	formation	1843:1851	formation of dental biofilms and caries development	1843:1893	Further studies are necessary to determine implications for formation of dental biofilms and caries development.
24615298	5	5	theme	cellular	1099:1106	arg1	Release					969:975	Release	969:975	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)	969:1068	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	5	theme	cellular	1099:1106	arg1	viability					1108:1116	the cellular viability	1095:1116	the cellular viability	1095:1116	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	7	6	theme	weak	1359:1362	arg1	trend					1376:1380	a weak synergistic trend	1357:1380	a weak synergistic trend	1357:1380	Moreover, co-exposure to TEGDMA and both types of filler particles also resulted in an additive attenuation, although with a weak synergistic trend.
24615298	9	7	theme	risk	1615:1618	arg1	assessments					1620:1630	future risk assessments	1608:1630	future risk assessments	1608:1630	CONCLUSION The present findings emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments.
24615298	4	8	theme	h	837:837	arg1	exposure					848:855	a subsequent 24 h combined exposure	821:855	a subsequent 24 h combined exposure to monomers and/or particles	821:884	RAW 264.7 macrophages were exposed to monomers and/or particles for 24 h, with a subsequent 24 h combined exposure to monomers and/or particles and the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response.
24615298	6	9	theme	Co-exposure	1127:1137	arg1	RESULTS					1119:1125	RESULTS	1119:1125	RESULTS Co-exposure to Nanosilica and Quartz	1119:1162	RESULTS Co-exposure to Nanosilica and Quartz resulted in an additive attenuation of the LPS-induced IL-1β release.
24615298	0	10	theme	line	126:129	arg1	RAW					131:133	macrophage cell line RAW 264.7	110:139	the macrophage cell line RAW 264.7	106:139	TEGDMA and filler particles from dental composites additively attenuate LPS-induced cytokine release from the macrophage cell line RAW 264.7.
24615298	10	11	theme	immune	1723:1728	arg1	response					1730:1737	the macrophage immune response	1708:1737	the macrophage immune response	1708:1737	CLINICAL RELEVANCE Attenuated cytokine release could have implications for the macrophage immune response and result in impaired bacterial clearance.
24615298	7	12	theme	particles	1291:1299	arg1	types					1275:1279	both types	1270:1279	both types of filler particles	1270:1299	Moreover, co-exposure to TEGDMA and both types of filler particles also resulted in an additive attenuation, although with a weak synergistic trend.
24615298	7	12	theme	particles	1291:1299	arg1	co-exposure					1244:1254	co-exposure	1244:1254	co-exposure to TEGDMA	1244:1264	Moreover, co-exposure to TEGDMA and both types of filler particles also resulted in an additive attenuation, although with a weak synergistic trend.
24615298	5	13	theme	cytokines	1001:1009	arg1	Release					969:975	Release	969:975	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)	969:1068	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	13	theme	cytokines	1001:1009	arg1	viability					1108:1116	the cellular viability	1095:1116	the cellular viability	1095:1116	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	10	14	theme	bacterial	1762:1770	arg1	clearance					1772:1780	impaired bacterial clearance	1753:1780	impaired bacterial clearance	1753:1780	CLINICAL RELEVANCE Attenuated cytokine release could have implications for the macrophage immune response and result in impaired bacterial clearance.
24615298	6	15	theme	release	1225:1231	arg1	attenuation					1188:1198	an additive attenuation	1176:1198	an additive attenuation of the LPS-induced IL-1β release	1176:1231	RESULTS Co-exposure to Nanosilica and Quartz resulted in an additive attenuation of the LPS-induced IL-1β release.
24615298	4	16	theme	subsequent	823:832	arg1	exposure					848:855	a subsequent 24 h combined exposure	821:855	a subsequent 24 h combined exposure to monomers and/or particles	821:884	RAW 264.7 macrophages were exposed to monomers and/or particles for 24 h, with a subsequent 24 h combined exposure to monomers and/or particles and the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response.
24615298	6	17	theme	LPS-induced	1207:1217	arg1	release					1225:1231	the LPS-induced IL-1β release	1203:1231	the LPS-induced IL-1β release	1203:1231	RESULTS Co-exposure to Nanosilica and Quartz resulted in an additive attenuation of the LPS-induced IL-1β release.
24615298	10	18	theme	macrophage	1712:1721	arg1	response					1730:1737	the macrophage immune response	1708:1737	the macrophage immune response	1708:1737	CLINICAL RELEVANCE Attenuated cytokine release could have implications for the macrophage immune response and result in impaired bacterial clearance.
24615298	2	19	theme	immune	496:501	arg1	response					503:510	the macrophage immune response	481:510	the macrophage immune response	481:510	The objective of the present study was to investigate if combined exposures to particles and a methacrylate monomer from composite fillings resulted in additive effects on the macrophage immune response.
24615298	8	20	theme	cellular	1387:1394	arg1	viability					1396:1404	The cellular viability	1383:1404	The cellular viability	1383:1404	The cellular viability and TNF-α release were not significantly affected by the exposures.
24615298	5	21	theme	tumor	1038:1042	arg1	cytokines					1001:1009	the pro-inflammatory cytokines	980:1009	the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)	980:1068	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	21	theme	tumor	1038:1042	arg1	TNF-α					1063:1067	TNF-α	1063:1067	TNF-α	1063:1067	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	21	theme	tumor	1038:1042	arg1	factor-α					1053:1060	tumor necrosis factor-α	1038:1060	tumor necrosis factor-α (TNF-α)	1038:1068	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	1	22	theme	Due	153:155	arg1	OBJECTIVES					142:151	OBJECTIVES	142:151	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity	142:227	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	4	23	theme	factor	904:909	arg1	LPS					931:933	LPS	931:933	LPS	931:933	RAW 264.7 macrophages were exposed to monomers and/or particles for 24 h, with a subsequent 24 h combined exposure to monomers and/or particles and the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response.
24615298	4	23	theme	factor	904:909	arg1	lipopolysaccharide					911:928	the bacterial factor lipopolysaccharide	890:928	the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response	890:966	RAW 264.7 macrophages were exposed to monomers and/or particles for 24 h, with a subsequent 24 h combined exposure to monomers and/or particles and the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response.
24615298	0	24	theme	cytokine	84:91	arg1	release					93:99	LPS-induced cytokine release	72:99	LPS-induced cytokine release from the macrophage cell line RAW 264.7	72:139	TEGDMA and filler particles from dental composites additively attenuate LPS-induced cytokine release from the macrophage cell line RAW 264.7.
24615298	1	25	theme	incomplete	160:169	arg1	cells					204:208	cells	204:208	cells in the oral cavity	204:227	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	1	25	theme	incomplete	160:169	arg1	degradation					191:201	incomplete curing and material degradation	160:201	incomplete curing and material degradation	160:201	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	0	26	from	composites	40:49	arg1	particles					18:26	TEGDMA and filler particles	0:26	TEGDMA and filler particles from dental composites	0:49	TEGDMA and filler particles from dental composites additively attenuate LPS-induced cytokine release from the macrophage cell line RAW 264.7.
24615298	11	27	theme	caries	1876:1881	arg1	development					1883:1893	caries development	1876:1893	caries development	1876:1893	Further studies are necessary to determine implications for formation of dental biofilms and caries development.
24615298	0	28	theme	macrophage	110:119	arg1	RAW					131:133	macrophage cell line RAW 264.7	110:139	the macrophage cell line RAW 264.7	106:139	TEGDMA and filler particles from dental composites additively attenuate LPS-induced cytokine release from the macrophage cell line RAW 264.7.
24615298	2	29	theme	combined	366:373	arg1	exposures					375:383	combined exposures	366:383	combined exposures to particles and a methacrylate monomer from composite fillings	366:447	The objective of the present study was to investigate if combined exposures to particles and a methacrylate monomer from composite fillings resulted in additive effects on the macrophage immune response.
24615298	0	30	theme	TEGDMA	0:5	arg1	particles					18:26	TEGDMA and filler particles	0:26	TEGDMA and filler particles from dental composites	0:49	TEGDMA and filler particles from dental composites additively attenuate LPS-induced cytokine release from the macrophage cell line RAW 264.7.
24615298	1	31	theme	filler	260:265	arg1	particles					267:275	filler particles	260:275	filler particles	260:275	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	9	32	theme	present	1489:1495	arg1	findings					1497:1504	The present findings	1485:1504	The present findings	1485:1504	CONCLUSION The present findings emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments.
24615298	4	33	theme	immune	952:957	arg1	response					959:966	an immune response	949:966	an immune response	949:966	RAW 264.7 macrophages were exposed to monomers and/or particles for 24 h, with a subsequent 24 h combined exposure to monomers and/or particles and the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response.
24615298	2	34	theme	methacrylate	404:415	arg1	monomer					417:423	a methacrylate monomer	402:423	a methacrylate monomer	402:423	The objective of the present study was to investigate if combined exposures to particles and a methacrylate monomer from composite fillings resulted in additive effects on the macrophage immune response.
24615298	0	35	theme	filler	11:16	arg1	particles					18:26	TEGDMA and filler particles	0:26	TEGDMA and filler particles from dental composites	0:49	TEGDMA and filler particles from dental composites additively attenuate LPS-induced cytokine release from the macrophage cell line RAW 264.7.
24615298	1	36	theme	composite	289:297	arg1	fillings					299:306	dental composite fillings	282:306	dental composite fillings	282:306	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	2	37	theme	present	330:336	arg1	study					338:342	the present study	326:342	the present study	326:342	The objective of the present study was to investigate if combined exposures to particles and a methacrylate monomer from composite fillings resulted in additive effects on the macrophage immune response.
24615298	9	38	theme	considering	1533:1543	arg1	effects					1545:1551	considering effects	1533:1551	considering effects of combined exposure to dental degradation products	1533:1603	CONCLUSION The present findings emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments.
24615298	10	39	theme	CLINICAL	1633:1640	arg1	RELEVANCE					1642:1650	CLINICAL RELEVANCE	1633:1650	CLINICAL RELEVANCE Attenuated cytokine release	1633:1678	CLINICAL RELEVANCE Attenuated cytokine release could have implications for the macrophage immune response and result in impaired bacterial clearance.
24615298	1	40	theme	oral	217:220	arg1	cavity					222:227	the oral cavity	213:227	the oral cavity	213:227	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	3	41	theme	0.5-4	625:629	arg1	2					637:637	2	637:637	2	637:637	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	41	theme	0.5-4	625:629	arg1	μg/cm					631:635	0.5-4 μg/cm	625:635	concentrations 0.5-4 μg/cm(2)	610:638	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	42	theme	MATERIAL	513:520	arg1	Quartz					579:584	Quartz	579:584	Quartz (1 μm)	579:591	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	42	theme	MATERIAL	513:520	arg1	Nanosilica					556:565	Nanosilica	556:565	Nanosilica (12 nm)	556:573	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	42	theme	MATERIAL	513:520	arg1	particles					545:553	MATERIAL AND METHODS Two filler particles	513:553	MATERIAL AND METHODS Two filler particles	513:553	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	9	43	theme	exposure	1565:1572	arg1	effects					1545:1551	considering effects	1533:1551	considering effects of combined exposure to dental degradation products	1533:1603	CONCLUSION The present findings emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments.
24615298	3	44	dep	concentrations	610:623	arg1	2					637:637	2	637:637	2	637:637	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	44	dep	concentrations	610:623	arg1	μg/cm					631:635	0.5-4 μg/cm	625:635	concentrations 0.5-4 μg/cm(2)	610:638	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	9	45	theme	dental	1577:1582	arg1	products					1596:1603	dental degradation products	1577:1603	dental degradation products	1577:1603	CONCLUSION The present findings emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments.
24615298	2	46	theme	study	338:342	arg1	objective					313:321	The objective	309:321	The objective of the present study	309:342	The objective of the present study was to investigate if combined exposures to particles and a methacrylate monomer from composite fillings resulted in additive effects on the macrophage immune response.
24615298	3	47	theme	filler	538:543	arg1	Quartz					579:584	Quartz	579:584	Quartz (1 μm)	579:591	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	47	theme	filler	538:543	arg1	Nanosilica					556:565	Nanosilica	556:565	Nanosilica (12 nm)	556:573	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	47	theme	filler	538:543	arg1	particles					545:553	MATERIAL AND METHODS Two filler particles	513:553	MATERIAL AND METHODS Two filler particles	513:553	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	7	48	theme	synergistic	1364:1374	arg1	trend					1376:1380	a weak synergistic trend	1357:1380	a weak synergistic trend	1357:1380	Moreover, co-exposure to TEGDMA and both types of filler particles also resulted in an additive attenuation, although with a weak synergistic trend.
24615298	6	49	theme	additive	1179:1186	arg1	attenuation					1188:1198	an additive attenuation	1176:1198	an additive attenuation of the LPS-induced IL-1β release	1176:1231	RESULTS Co-exposure to Nanosilica and Quartz resulted in an additive attenuation of the LPS-induced IL-1β release.
24615298	3	50	theme	triethyleneglycol	672:688	arg1	TEGDMA					706:711	TEGDMA	706:711	TEGDMA	706:711	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	50	theme	triethyleneglycol	672:688	arg1	dimethacrylate					690:703	the methacrylate monomer triethyleneglycol dimethacrylate	647:703	the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA)	647:712	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	51	theme	METHODS	526:532	arg1	Quartz					579:584	Quartz	579:584	Quartz (1 μm)	579:591	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	51	theme	METHODS	526:532	arg1	Nanosilica					556:565	Nanosilica	556:565	Nanosilica (12 nm)	556:573	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	51	theme	METHODS	526:532	arg1	particles					545:553	MATERIAL AND METHODS Two filler particles	513:553	MATERIAL AND METHODS Two filler particles	513:553	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	1	52	theme	curing	171:176	arg1	cells					204:208	cells	204:208	cells in the oral cavity	204:227	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	1	52	theme	curing	171:176	arg1	degradation					191:201	incomplete curing and material degradation	160:201	incomplete curing and material degradation	160:201	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	9	53	theme	future	1608:1613	arg1	assessments					1620:1630	future risk assessments	1608:1630	future risk assessments	1608:1630	CONCLUSION The present findings emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments.
24615298	3	54	theme	methacrylate	651:662	arg1	TEGDMA					706:711	TEGDMA	706:711	TEGDMA	706:711	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	3	54	theme	methacrylate	651:662	arg1	dimethacrylate					690:703	the methacrylate monomer triethyleneglycol dimethacrylate	647:703	the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA)	647:712	MATERIAL AND METHODS Two filler particles, Nanosilica (12 nm) and Quartz (1 μm), were studied at concentrations 0.5-4 μg/cm(2), while the methacrylate monomer triethyleneglycol dimethacrylate (TEGDMA) was applied at 5 and 50 μM.
24615298	1	55	theme	material	182:189	arg1	cells					204:208	cells	204:208	cells in the oral cavity	204:227	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	1	55	theme	material	182:189	arg1	degradation					191:201	incomplete curing and material degradation	160:201	incomplete curing and material degradation	160:201	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	7	56	theme	additive	1321:1328	arg1	attenuation					1330:1340	an additive attenuation	1318:1340	an additive attenuation	1318:1340	Moreover, co-exposure to TEGDMA and both types of filler particles also resulted in an additive attenuation, although with a weak synergistic trend.
24615298	4	57	theme	24	834:835	arg1	h					837:837	h	837:837	h	837:837	RAW 264.7 macrophages were exposed to monomers and/or particles for 24 h, with a subsequent 24 h combined exposure to monomers and/or particles and the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response.
24615298	5	58	theme	pro-inflammatory	984:999	arg1	factor-α					1053:1060	tumor necrosis factor-α	1038:1060	tumor necrosis factor-α (TNF-α)	1038:1068	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	58	theme	pro-inflammatory	984:999	arg1	cytokines					1001:1009	the pro-inflammatory cytokines	980:1009	the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)	980:1068	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	58	theme	pro-inflammatory	984:999	arg1	interleukin-1β					1011:1024	interleukin-1β	1011:1024	interleukin-1β (IL-1β)	1011:1032	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	4	59	theme	combined	839:846	arg1	exposure					848:855	a subsequent 24 h combined exposure	821:855	a subsequent 24 h combined exposure to monomers and/or particles	821:884	RAW 264.7 macrophages were exposed to monomers and/or particles for 24 h, with a subsequent 24 h combined exposure to monomers and/or particles and the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response.
24615298	10	60	theme	impaired	1753:1760	arg1	clearance					1772:1780	impaired bacterial clearance	1753:1780	impaired bacterial clearance	1753:1780	CLINICAL RELEVANCE Attenuated cytokine release could have implications for the macrophage immune response and result in impaired bacterial clearance.
24615298	7	61	theme	filler	1284:1289	arg1	particles					1291:1299	filler particles	1284:1299	filler particles	1284:1299	Moreover, co-exposure to TEGDMA and both types of filler particles also resulted in an additive attenuation, although with a weak synergistic trend.
24615298	9	62	dep	CONCLUSION	1474:1483	arg1	emphasize					1506:1514	emphasize	1506:1514	emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments	1506:1630	CONCLUSION The present findings emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments.
24615298	11	63	theme	Further	1783:1789	arg1	studies					1791:1797	Further studies	1783:1797	Further studies	1783:1797	Further studies are necessary to determine implications for formation of dental biofilms and caries development.
24615298	6	64	theme	IL-1β	1219:1223	arg1	release					1225:1231	the LPS-induced IL-1β release	1203:1231	the LPS-induced IL-1β release	1203:1231	RESULTS Co-exposure to Nanosilica and Quartz resulted in an additive attenuation of the LPS-induced IL-1β release.
24615298	1	65	from	cells	204:208	arg1	cavity					222:227	the oral cavity	213:227	the oral cavity	213:227	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	10	66	contain	have	1686:1689	arg1	RELEVANCE					1642:1650	CLINICAL RELEVANCE	1633:1650	CLINICAL RELEVANCE Attenuated cytokine release	1633:1678	CLINICAL RELEVANCE Attenuated cytokine release could have implications for the macrophage immune response and result in impaired bacterial clearance.
24615298	10	66	contain	have	1686:1689	arg2	implications					1691:1702	implications	1691:1702	implications for the macrophage immune response	1691:1737	CLINICAL RELEVANCE Attenuated cytokine release could have implications for the macrophage immune response and result in impaired bacterial clearance.
24615298	0	67	theme	LPS-induced	72:82	arg1	release					93:99	LPS-induced cytokine release	72:99	LPS-induced cytokine release from the macrophage cell line RAW 264.7	72:139	TEGDMA and filler particles from dental composites additively attenuate LPS-induced cytokine release from the macrophage cell line RAW 264.7.
24615298	2	68	theme	macrophage	485:494	arg1	response					503:510	the macrophage immune response	481:510	the macrophage immune response	481:510	The objective of the present study was to investigate if combined exposures to particles and a methacrylate monomer from composite fillings resulted in additive effects on the macrophage immune response.
24615298	8	69	theme	TNF-α	1410:1414	arg1	release					1416:1422	TNF-α release	1410:1422	TNF-α release	1410:1422	The cellular viability and TNF-α release were not significantly affected by the exposures.
24615298	5	70	theme	necrosis	1044:1051	arg1	cytokines					1001:1009	the pro-inflammatory cytokines	980:1009	the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)	980:1068	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	70	theme	necrosis	1044:1051	arg1	TNF-α					1063:1067	TNF-α	1063:1067	TNF-α	1063:1067	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	70	theme	necrosis	1044:1051	arg1	factor-α					1053:1060	tumor necrosis factor-α	1038:1060	tumor necrosis factor-α (TNF-α)	1038:1068	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	4	71	theme	bacterial	894:902	arg1	LPS					931:933	LPS	931:933	LPS	931:933	RAW 264.7 macrophages were exposed to monomers and/or particles for 24 h, with a subsequent 24 h combined exposure to monomers and/or particles and the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response.
24615298	4	71	theme	bacterial	894:902	arg1	lipopolysaccharide					911:928	the bacterial factor lipopolysaccharide	890:928	the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response	890:966	RAW 264.7 macrophages were exposed to monomers and/or particles for 24 h, with a subsequent 24 h combined exposure to monomers and/or particles and the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response.
24615298	2	72	from	fillings	440:447	arg1	exposures					375:383	combined exposures	366:383	combined exposures to particles and a methacrylate monomer from composite fillings	366:447	The objective of the present study was to investigate if combined exposures to particles and a methacrylate monomer from composite fillings resulted in additive effects on the macrophage immune response.
24615298	0	73	theme	cell	121:124	arg1	RAW					131:133	macrophage cell line RAW 264.7	110:139	the macrophage cell line RAW 264.7	106:139	TEGDMA and filler particles from dental composites additively attenuate LPS-induced cytokine release from the macrophage cell line RAW 264.7.
24615298	2	74	from	effects	470:476	arg1	response					503:510	the macrophage immune response	481:510	the macrophage immune response	481:510	The objective of the present study was to investigate if combined exposures to particles and a methacrylate monomer from composite fillings resulted in additive effects on the macrophage immune response.
24615298	11	75	theme	development	1883:1893	arg1	formation					1843:1851	formation	1843:1851	formation of dental biofilms and caries development	1843:1893	Further studies are necessary to determine implications for formation of dental biofilms and caries development.
24615298	2	76	theme	composite	430:438	arg1	fillings					440:447	composite fillings	430:447	composite fillings	430:447	The objective of the present study was to investigate if combined exposures to particles and a methacrylate monomer from composite fillings resulted in additive effects on the macrophage immune response.
24615298	0	77	theme	dental	33:38	arg1	composites					40:49	dental composites	33:49	dental composites	33:49	TEGDMA and filler particles from dental composites additively attenuate LPS-induced cytokine release from the macrophage cell line RAW 264.7.
24615298	1	78	theme	dental	282:287	arg1	fillings					299:306	dental composite fillings	282:306	dental composite fillings	282:306	OBJECTIVES Due to incomplete curing and material degradation, cells in the oral cavity may be exposed to monomers and filler particles from dental composite fillings.
24615298	9	79	from	necessity	1520:1528	arg1	assessments					1620:1630	future risk assessments	1608:1630	future risk assessments	1608:1630	CONCLUSION The present findings emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments.
24615298	2	80	theme	additive	461:468	arg1	effects					470:476	additive effects	461:476	additive effects on the macrophage immune response	461:510	The objective of the present study was to investigate if combined exposures to particles and a methacrylate monomer from composite fillings resulted in additive effects on the macrophage immune response.
24615298	4	81	theme	RAW	742:744	arg1	macrophages					752:762	RAW 264.7 macrophages	742:762	RAW 264.7 macrophages	742:762	RAW 264.7 macrophages were exposed to monomers and/or particles for 24 h, with a subsequent 24 h combined exposure to monomers and/or particles and the bacterial factor lipopolysaccharide (LPS) to stimulate an immune response.
24615298	9	82	theme	effects	1545:1551	arg1	necessity					1520:1528	the necessity	1516:1528	the necessity of considering effects of combined exposure to dental degradation products in future risk assessments	1516:1630	CONCLUSION The present findings emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments.
24615298	5	83	dep	cytokines	1001:1009	arg1	cytokines					1001:1009	the pro-inflammatory cytokines	980:1009	the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)	980:1068	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	83	dep	cytokines	1001:1009	arg1	TNF-α					1063:1067	TNF-α	1063:1067	TNF-α	1063:1067	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	83	dep	cytokines	1001:1009	arg1	factor-α					1053:1060	tumor necrosis factor-α	1038:1060	tumor necrosis factor-α (TNF-α)	1038:1068	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	83	dep	cytokines	1001:1009	arg1	IL-1β					1027:1031	IL-1β	1027:1031	IL-1β	1027:1031	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	5	83	dep	cytokines	1001:1009	arg1	interleukin-1β					1011:1024	interleukin-1β	1011:1024	interleukin-1β (IL-1β)	1011:1032	Release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as well as the cellular viability.
24615298	9	84	theme	combined	1556:1563	arg1	exposure					1565:1572	combined exposure	1556:1572	combined exposure to dental degradation products	1556:1603	CONCLUSION The present findings emphasize the necessity of considering effects of combined exposure to dental degradation products in future risk assessments.
24615298	0	85	from	RAW	131:133	arg1	release					93:99	LPS-induced cytokine release	72:99	LPS-induced cytokine release from the macrophage cell line RAW 264.7	72:139	TEGDMA and filler particles from dental composites additively attenuate LPS-induced cytokine release from the macrophage cell line RAW 264.7.
24615298	11	86	theme	dental	1856:1861	arg1	biofilms					1863:1870	dental biofilms	1856:1870	dental biofilms	1856:1870	Further studies are necessary to determine implications for formation of dental biofilms and caries development.
28157608	12	0	theme	bio-anode	1553:1561	arg1	type					1545:1548	new type	1541:1548	new type of bio-anode that have higher power density and can operate for long term	1541:1622	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	12	1	theme	higher	1573:1578	arg1	density					1586:1592	higher power density	1573:1592	higher power density	1573:1592	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	3	2	from	conductivity	485:496	arg1	gels					501:504	gels	501:504	gels	501:504	Increase of polyaniline concentration resulted an increase in the conductivity in gels.
28157608	3	3	from	increase	469:476	arg1	conductivity					485:496	the conductivity	481:496	the conductivity in gels	481:504	Increase of polyaniline concentration resulted an increase in the conductivity in gels.
28157608	0	4	theme	alginate/polyaniline/titanium-dioxide/graphite	74:119	arg1	composites					121:130	alginate/polyaniline/titanium-dioxide/graphite composites	74:130	alginate/polyaniline/titanium-dioxide/graphite composites	74:130	Formation of novel hydrogel bio-anode by immobilization of biocatalyst in alginate/polyaniline/titanium-dioxide/graphite composites and its electrical performance.
28157608	12	5	theme	fuel	1526:1529	arg1	cell					1531:1534	an improved microbial fuel cell	1504:1534	an improved microbial fuel cell	1504:1534	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	5	6	theme	power	715:719	arg1	density					721:727	4-fold higher power density	701:727	4-fold higher power density	701:727	Furthermore, addition of 0.05 g/mL graphite powder caused 10-fold higher conductivity and 4-fold higher power density, respectively.
28157608	11	7	theme	operation	1362:1370	arg1	mode					1372:1375	the continuous operation mode	1347:1375	the continuous operation mode of the microbial fuel cell	1347:1402	Similar power density was observed in the continuous operation mode of the microbial fuel cell, and it was operated stably for more than 7 days.
28157608	2	8	used	used	371:374	arg2	Alginate					310:317	Alginate	310:317	Alginate as dopant and template as well as entrapped gel	310:365	Alginate as dopant and template as well as entrapped gel was used for immobilization of microorganism cells.
28157608	11	9	theme	microbial	1384:1392	arg1	cell					1399:1402	the microbial fuel cell	1380:1402	the microbial fuel cell	1380:1402	Similar power density was observed in the continuous operation mode of the microbial fuel cell, and it was operated stably for more than 7 days.
28157608	4	10	theme	higher	576:581	arg1	conductivity					583:594	6-fold and 10-fold higher conductivity	557:594	6-fold and 10-fold higher conductivity	557:594	Addition of 0.01 and 0.02 g/mL polyaniline caused 6-fold and 10-fold higher conductivity, respectively.
28157608	8	11	theme	semi-	1072:1076	arg1	modes					1104:1108	semi- and continuous operations modes	1072:1108	semi- and continuous operations modes	1072:1108	Modified hydrogel anode was successfully used in microbial fuel cell systems both in semi- and continuous operations modes.
28157608	8	12	theme	microbial	1036:1044	arg1	systems					1056:1062	microbial fuel cell systems	1036:1062	microbial fuel cell systems	1036:1062	Modified hydrogel anode was successfully used in microbial fuel cell systems both in semi- and continuous operations modes.
28157608	7	13	theme	Optimized	866:874	arg1	concentrations					876:889	Optimized concentrations	866:889	Optimized concentrations of polyaniline and graphite powder	866:924	Optimized concentrations of polyaniline and graphite powder were determined to be 0.02 g/mL and 0.05 g/mL, respectively.
28157608	3	14	theme	polyaniline	431:441	arg1	concentration					443:455	polyaniline concentration	431:455	polyaniline concentration	431:455	Increase of polyaniline concentration resulted an increase in the conductivity in gels.
28157608	0	15	from	immobilization	41:54	arg1	composites					121:130	alginate/polyaniline/titanium-dioxide/graphite composites	74:130	alginate/polyaniline/titanium-dioxide/graphite composites	74:130	Formation of novel hydrogel bio-anode by immobilization of biocatalyst in alginate/polyaniline/titanium-dioxide/graphite composites and its electrical performance.
28157608	0	15	from	immobilization	41:54	arg1	performance					151:161	its electrical performance	136:161	its electrical performance	136:161	Formation of novel hydrogel bio-anode by immobilization of biocatalyst in alginate/polyaniline/titanium-dioxide/graphite composites and its electrical performance.
28157608	8	16	dep	systems	1056:1062	arg1	both					1064:1067	both	1064:1067	both	1064:1067	Modified hydrogel anode was successfully used in microbial fuel cell systems both in semi- and continuous operations modes.
28157608	6	17	theme	power-density	844:856	arg1	output					858:863	7-fold higher power-density output	830:863	7-fold higher power-density output	830:863	The combination of polyaniline and graphite resulted 105-fold higher conductivity and 7-fold higher power-density output.
28157608	1	18	theme	alginate/polyaniline/TiO2/graphite	217:250	arg1	composites					252:261	alginate/polyaniline/TiO2/graphite composites	217:261	alginate/polyaniline/TiO2/graphite composites	217:261	A new bio-anode containing gel-entrapped bacteria in alginate/polyaniline/TiO2/graphite composites was constructed and electrically investigated.
28157608	0	19	theme	electrical	140:149	arg1	performance					151:161	its electrical performance	136:161	its electrical performance	136:161	Formation of novel hydrogel bio-anode by immobilization of biocatalyst in alginate/polyaniline/titanium-dioxide/graphite composites and its electrical performance.
28157608	9	20	theme	semi-continuous	1114:1128	arg1	mode					1130:1133	semi-continuous mode	1114:1133	semi-continuous mode	1114:1133	In semi-continuous mode, about 7.88 W/m3 power density was obtained after 13 h of fermentation.
28157608	2	21	theme	entrapped	353:361	arg1	gel					363:365	entrapped gel	353:365	dopant and template as well as entrapped gel	322:365	Alginate as dopant and template as well as entrapped gel was used for immobilization of microorganism cells.
28157608	6	22	theme	higher	837:842	arg1	output					858:863	7-fold higher power-density output	830:863	7-fold higher power-density output	830:863	The combination of polyaniline and graphite resulted 105-fold higher conductivity and 7-fold higher power-density output.
28157608	9	23	theme	power	1152:1156	arg1	density					1158:1164	about 7.88 W/m3 power density	1136:1164	about 7.88 W/m3 power density	1136:1164	In semi-continuous mode, about 7.88 W/m3 power density was obtained after 13 h of fermentation.
28157608	1	24	contain	containing	180:189	arg2	bacteria					205:212	gel-entrapped bacteria	191:212	gel-entrapped bacteria	191:212	A new bio-anode containing gel-entrapped bacteria in alginate/polyaniline/TiO2/graphite composites was constructed and electrically investigated.
28157608	1	24	contain	containing	180:189	arg1	bio-anode					170:178	A new bio-anode	164:178	A new bio-anode containing gel-entrapped bacteria in alginate/polyaniline/TiO2/graphite composites	164:261	A new bio-anode containing gel-entrapped bacteria in alginate/polyaniline/TiO2/graphite composites was constructed and electrically investigated.
28157608	2	25	theme	microorganism	398:410	arg1	cells					412:416	microorganism cells	398:416	microorganism cells	398:416	Alginate as dopant and template as well as entrapped gel was used for immobilization of microorganism cells.
28157608	8	26	theme	fuel	1046:1049	arg1	systems					1056:1062	microbial fuel cell systems	1036:1062	microbial fuel cell systems	1036:1062	Modified hydrogel anode was successfully used in microbial fuel cell systems both in semi- and continuous operations modes.
28157608	12	27	contain	have	1568:1571	arg2	density					1586:1592	higher power density	1573:1592	higher power density	1573:1592	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	12	27	contain	have	1568:1571	arg1	bio-anode					1553:1561	bio-anode	1553:1561	bio-anode that have higher power density and can operate for long term	1553:1622	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	11	28	theme	fuel	1394:1397	arg1	cell					1399:1402	the microbial fuel cell	1380:1402	the microbial fuel cell	1380:1402	Similar power density was observed in the continuous operation mode of the microbial fuel cell, and it was operated stably for more than 7 days.
28157608	12	29	with	development	1489:1499	arg1	type					1545:1548	new type	1541:1548	new type of bio-anode that have higher power density and can operate for long term	1541:1622	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	12	30	theme	long	1614:1617	arg1	term					1619:1622	long term	1614:1622	long term	1614:1622	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	0	31	theme	hydrogel	19:26	arg1	bio-anode					28:36	novel hydrogel bio-anode	13:36	novel hydrogel bio-anode	13:36	Formation of novel hydrogel bio-anode by immobilization of biocatalyst in alginate/polyaniline/titanium-dioxide/graphite composites and its electrical performance.
28157608	5	32	theme	higher	708:713	arg1	density					721:727	4-fold higher power density	701:727	4-fold higher power density	701:727	Furthermore, addition of 0.05 g/mL graphite powder caused 10-fold higher conductivity and 4-fold higher power density, respectively.
28157608	12	33	theme	new	1541:1543	arg1	type					1545:1548	new type	1541:1548	new type of bio-anode that have higher power density and can operate for long term	1541:1622	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	11	34	theme	continuous	1351:1360	arg1	mode					1372:1375	the continuous operation mode	1347:1375	the continuous operation mode of the microbial fuel cell	1347:1402	Similar power density was observed in the continuous operation mode of the microbial fuel cell, and it was operated stably for more than 7 days.
28157608	0	35	theme	novel	13:17	arg1	bio-anode					28:36	novel hydrogel bio-anode	13:36	novel hydrogel bio-anode	13:36	Formation of novel hydrogel bio-anode by immobilization of biocatalyst in alginate/polyaniline/titanium-dioxide/graphite composites and its electrical performance.
28157608	6	36	theme	higher	806:811	arg1	conductivity					813:824	105-fold higher conductivity	797:824	105-fold higher conductivity	797:824	The combination of polyaniline and graphite resulted 105-fold higher conductivity and 7-fold higher power-density output.
28157608	11	37	theme	cell	1399:1402	arg1	mode					1372:1375	the continuous operation mode	1347:1375	the continuous operation mode of the microbial fuel cell	1347:1402	Similar power density was observed in the continuous operation mode of the microbial fuel cell, and it was operated stably for more than 7 days.
28157608	4	38	theme	polyaniline	538:548	arg1	Addition					507:514	Addition	507:514	Addition of 0.01 and 0.02 g/mL polyaniline	507:548	Addition of 0.01 and 0.02 g/mL polyaniline caused 6-fold and 10-fold higher conductivity, respectively.
28157608	11	39	theme	Similar	1309:1315	arg1	density					1323:1329	Similar power density	1309:1329	Similar power density	1309:1329	Similar power density was observed in the continuous operation mode of the microbial fuel cell, and it was operated stably for more than 7 days.
28157608	8	40	theme	Modified	987:994	arg1	anode					1005:1009	Modified hydrogel anode	987:1009	Modified hydrogel anode	987:1009	Modified hydrogel anode was successfully used in microbial fuel cell systems both in semi- and continuous operations modes.
28157608	5	41	theme	graphite	646:653	arg1	powder					655:660	0.05 g/mL graphite powder	636:660	0.05 g/mL graphite powder	636:660	Furthermore, addition of 0.05 g/mL graphite powder caused 10-fold higher conductivity and 4-fold higher power density, respectively.
28157608	0	42	theme	bio-anode	28:36	arg1	Formation					0:8	Formation	0:8	Formation of novel hydrogel bio-anode by immobilization of biocatalyst in alginate/polyaniline/titanium-dioxide/graphite composites and its electrical performance.	0:162	Formation of novel hydrogel bio-anode by immobilization of biocatalyst in alginate/polyaniline/titanium-dioxide/graphite composites and its electrical performance.
28157608	10	43	theme	immobilized	1290:1300	arg1	cells					1302:1306	immobilized cells	1290:1306	immobilized cells	1290:1306	The glucose consumption rate was calculated to be about 7 mg glucose/h/1.2·107 CFU immobilized cells.
28157608	11	44	theme	power	1317:1321	arg1	density					1323:1329	Similar power density	1309:1329	Similar power density	1309:1329	Similar power density was observed in the continuous operation mode of the microbial fuel cell, and it was operated stably for more than 7 days.
28157608	5	45	theme	powder	655:660	arg1	addition					624:631	addition	624:631	addition of 0.05 g/mL graphite powder	624:660	Furthermore, addition of 0.05 g/mL graphite powder caused 10-fold higher conductivity and 4-fold higher power density, respectively.
28157608	3	46	theme	concentration	443:455	arg1	Increase					419:426	Increase	419:426	Increase of polyaniline concentration	419:455	Increase of polyaniline concentration resulted an increase in the conductivity in gels.
28157608	11	47	located	observed	1335:1342	arg1	mode					1372:1375	the continuous operation mode	1347:1375	the continuous operation mode of the microbial fuel cell	1347:1402	Similar power density was observed in the continuous operation mode of the microbial fuel cell, and it was operated stably for more than 7 days.
28157608	11	47	located	observed	1335:1342	arg2	density					1323:1329	Similar power density	1309:1329	Similar power density	1309:1329	Similar power density was observed in the continuous operation mode of the microbial fuel cell, and it was operated stably for more than 7 days.
28157608	12	48	theme	microbial	1516:1524	arg1	cell					1531:1534	an improved microbial fuel cell	1504:1534	an improved microbial fuel cell	1504:1534	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	12	49	theme	cell	1531:1534	arg1	development					1489:1499	development	1489:1499	development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term	1489:1622	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	12	50	theme	improved	1507:1514	arg1	cell					1531:1534	an improved microbial fuel cell	1504:1534	an improved microbial fuel cell	1504:1534	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	9	51	theme	fermentation	1193:1204	arg1	13 h					1185:1188	13 h	1185:1188	13 h of fermentation	1185:1204	In semi-continuous mode, about 7.88 W/m3 power density was obtained after 13 h of fermentation.
28157608	12	52	theme	power	1580:1584	arg1	density					1586:1592	higher power density	1573:1592	higher power density	1573:1592	Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
28157608	10	53	theme	consumption	1219:1229	arg1	rate					1231:1234	The glucose consumption rate	1207:1234	The glucose consumption rate	1207:1234	The glucose consumption rate was calculated to be about 7 mg glucose/h/1.2·107 CFU immobilized cells.
28157608	5	54	theme	higher	677:682	arg1	conductivity					684:695	10-fold higher conductivity	669:695	10-fold higher conductivity	669:695	Furthermore, addition of 0.05 g/mL graphite powder caused 10-fold higher conductivity and 4-fold higher power density, respectively.
28157608	1	55	theme	new	166:168	arg1	bio-anode					170:178	A new bio-anode	164:178	A new bio-anode containing gel-entrapped bacteria in alginate/polyaniline/TiO2/graphite composites	164:261	A new bio-anode containing gel-entrapped bacteria in alginate/polyaniline/TiO2/graphite composites was constructed and electrically investigated.
28157608	6	56	theme	graphite	779:786	arg1	combination					748:758	The combination	744:758	The combination of polyaniline and graphite	744:786	The combination of polyaniline and graphite resulted 105-fold higher conductivity and 7-fold higher power-density output.
28157608	7	57	theme	graphite	910:917	arg1	powder					919:924	graphite powder	910:924	graphite powder	910:924	Optimized concentrations of polyaniline and graphite powder were determined to be 0.02 g/mL and 0.05 g/mL, respectively.
28157608	8	58	theme	operations	1093:1102	arg1	modes					1104:1108	semi- and continuous operations modes	1072:1108	semi- and continuous operations modes	1072:1108	Modified hydrogel anode was successfully used in microbial fuel cell systems both in semi- and continuous operations modes.
28157608	10	59	theme	glucose	1211:1217	arg1	rate					1231:1234	The glucose consumption rate	1207:1234	The glucose consumption rate	1207:1234	The glucose consumption rate was calculated to be about 7 mg glucose/h/1.2·107 CFU immobilized cells.
28157608	8	60	theme	continuous	1082:1091	arg1	modes					1104:1108	semi- and continuous operations modes	1072:1108	semi- and continuous operations modes	1072:1108	Modified hydrogel anode was successfully used in microbial fuel cell systems both in semi- and continuous operations modes.
28157608	2	61	theme	cells	412:416	arg1	immobilization					380:393	immobilization	380:393	immobilization of microorganism cells	380:416	Alginate as dopant and template as well as entrapped gel was used for immobilization of microorganism cells.
28157608	8	62	theme	hydrogel	996:1003	arg1	anode					1005:1009	Modified hydrogel anode	987:1009	Modified hydrogel anode	987:1009	Modified hydrogel anode was successfully used in microbial fuel cell systems both in semi- and continuous operations modes.
28157608	7	63	theme	powder	919:924	arg1	concentrations					876:889	Optimized concentrations	866:889	Optimized concentrations of polyaniline and graphite powder	866:924	Optimized concentrations of polyaniline and graphite powder were determined to be 0.02 g/mL and 0.05 g/mL, respectively.
28157608	8	64	used	used	1028:1031	arg2	anode					1005:1009	Modified hydrogel anode	987:1009	Modified hydrogel anode	987:1009	Modified hydrogel anode was successfully used in microbial fuel cell systems both in semi- and continuous operations modes.
28157608	6	65	theme	polyaniline	763:773	arg1	combination					748:758	The combination	744:758	The combination of polyaniline and graphite	744:786	The combination of polyaniline and graphite resulted 105-fold higher conductivity and 7-fold higher power-density output.
28157608	7	66	theme	polyaniline	894:904	arg1	concentrations					876:889	Optimized concentrations	866:889	Optimized concentrations of polyaniline and graphite powder	866:924	Optimized concentrations of polyaniline and graphite powder were determined to be 0.02 g/mL and 0.05 g/mL, respectively.
28157608	0	67	theme	biocatalyst	59:69	arg1	immobilization					41:54	immobilization	41:54	immobilization of biocatalyst in alginate/polyaniline/titanium-dioxide/graphite composites and its electrical performance	41:161	Formation of novel hydrogel bio-anode by immobilization of biocatalyst in alginate/polyaniline/titanium-dioxide/graphite composites and its electrical performance.
28157608	8	68	theme	cell	1051:1054	arg1	systems					1056:1062	microbial fuel cell systems	1036:1062	microbial fuel cell systems	1036:1062	Modified hydrogel anode was successfully used in microbial fuel cell systems both in semi- and continuous operations modes.
28157608	5	69	theme	0.05 g/mL	636:644	arg1	powder					655:660	0.05 g/mL graphite powder	636:660	0.05 g/mL graphite powder	636:660	Furthermore, addition of 0.05 g/mL graphite powder caused 10-fold higher conductivity and 4-fold higher power density, respectively.
28157608	1	70	theme	gel-entrapped	191:203	arg1	bacteria					205:212	gel-entrapped bacteria	191:212	gel-entrapped bacteria	191:212	A new bio-anode containing gel-entrapped bacteria in alginate/polyaniline/TiO2/graphite composites was constructed and electrically investigated.
26876841	7	0	theme	EtOH	1002:1005	arg1	concentration					985:997	the concentration	981:997	the concentration of EtOH in grafting solution	981:1026	The results confirm that the concentration of EtOH in grafting solution significantly affects grafting efficiency of GA on chitosan.
26876841	2	1	theme	-carbodiimide	466:478	arg1	NHS					508:510	NHS	508:510	NHS	508:510	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	1	theme	-carbodiimide	466:478	arg1	/N-hydroxysuccinimide					485:505	1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide	433:505	1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS)	433:511	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	2	from	%	340:340	arg1	water					364:368	water	364:368	water	364:368	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	1	3	theme	chitosan	132:139	arg1	activity					120:127	The primary antioxidant (AOX) activity	90:127	The primary antioxidant (AOX) activity of chitosan	90:139	The primary antioxidant (AOX) activity of chitosan can be introduced by grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups.
26876841	2	4	theme	grafting	388:395	arg1	GA					397:398	grafting GA	388:398	grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS)	388:511	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	5	theme	3-dimethylaminopropyl	444:464	arg1	EDC					481:483	EDC	481:483	EDC	481:483	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	5	theme	3-dimethylaminopropyl	444:464	arg1	-carbodiimide					466:478	1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide	433:478	1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS)	433:511	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	6	6	theme	intermediate	930:941	arg1	ester					949:953	the intermediate - NHS ester	926:953	the intermediate - NHS ester	926:953	NMR studies showed that EtOH inhibited grafting of GA by prohibiting the production of the intermediate - NHS ester.
26876841	4	7	theme	GA/g	733:736	arg1	extent					717:722	the largest extent	705:722	the largest extent (285.9mg GA/g chitosan)	705:746	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	4	7	theme	GA/g	733:736	arg1	chitosan					738:745	285.9mg GA/g chitosan	725:745	285.9mg GA/g chitosan	725:745	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	6	8	theme	GA	890:891	arg1	grafting					878:885	grafting	878:885	grafting of GA	878:891	NMR studies showed that EtOH inhibited grafting of GA by prohibiting the production of the intermediate - NHS ester.
26876841	7	9	theme	GA	1073:1074	arg1	efficiency					1059:1068	grafting efficiency	1050:1068	grafting efficiency of GA on chitosan	1050:1086	The results confirm that the concentration of EtOH in grafting solution significantly affects grafting efficiency of GA on chitosan.
26876841	2	10	from	%	350:350	arg1	water					364:368	water	364:368	water	364:368	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	11	theme	/N-hydroxysuccinimide	485:505	arg1	presence					421:428	the presence	417:428	the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS)	417:511	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	7	12	from	efficiency	1059:1068	arg1	chitosan					1079:1086	chitosan	1079:1086	chitosan	1079:1086	The results confirm that the concentration of EtOH in grafting solution significantly affects grafting efficiency of GA on chitosan.
26876841	4	13	theme	deionized	621:629	arg1	water					631:635	pure deionized water	616:635	pure deionized water	616:635	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	4	13	theme	deionized	621:629	arg1	solvent					656:662	a sole solvent	649:662	a sole solvent (0% EtOH)	649:672	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	2	14	theme	concentration	324:336	arg1	effect					299:304	the effect	295:304	the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS)	295:511	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	4	15	theme	0	665:665	arg1	%					666:666	%	666:666	%	666:666	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	2	16	from	effect	299:304	arg1	efficiency					374:383	efficiency	374:383	efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS)	374:511	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	4	17	theme	pure	616:619	arg1	water					631:635	pure deionized water	616:635	pure deionized water	616:635	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	4	17	theme	pure	616:619	arg1	solvent					656:662	a sole solvent	649:662	a sole solvent (0% EtOH)	649:672	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	6	18	theme	ester	949:953	arg1	production					912:921	the production	908:921	the production of the intermediate - NHS ester	908:953	NMR studies showed that EtOH inhibited grafting of GA by prohibiting the production of the intermediate - NHS ester.
26876841	3	19	theme	total	594:598	arg1	phenolics					600:608	Folin's total phenolics	586:608	Folin's total phenolics	586:608	The grafting was confirmed by FTIR and the efficiency was quantified as Folin's total phenolics.
26876841	4	20	theme	%	666:666	arg1	EtOH					668:671	0% EtOH	665:671	0% EtOH	665:671	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	4	20	theme	%	666:666	arg1	solvent					656:662	a sole solvent	649:662	a sole solvent (0% EtOH)	649:672	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	4	21	used	used	641:644	arg2	water					631:635	pure deionized water	616:635	pure deionized water	616:635	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	4	21	used	used	641:644	arg2	solvent					656:662	a sole solvent	649:662	a sole solvent (0% EtOH)	649:672	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	0	22	theme	solvent	14:20	arg1	composition					22:32	solvent composition	14:32	solvent composition	14:32	The effect of solvent composition on grafting gallic acid onto chitosan via carbodiimide.
26876841	1	23	theme	hydroxyl	231:238	arg1	groups					240:245	its amino and/or hydroxyl groups	214:245	groups	240:245	The primary antioxidant (AOX) activity of chitosan can be introduced by grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups.
26876841	7	24	theme	grafting	1050:1057	arg1	efficiency					1059:1068	grafting efficiency	1050:1068	grafting efficiency of GA on chitosan	1050:1086	The results confirm that the concentration of EtOH in grafting solution significantly affects grafting efficiency of GA on chitosan.
26876841	6	25	theme	NMR	839:841	arg1	studies					843:849	NMR studies	839:849	NMR studies	839:849	NMR studies showed that EtOH inhibited grafting of GA by prohibiting the production of the intermediate - NHS ester.
26876841	5	26	theme	grafting	793:800	arg1	efficiency					802:811	the grafting efficiency	789:811	the grafting efficiency	789:811	As the concentration of EtOH increased, the grafting efficiency proportionally decreased.
26876841	6	27	theme	NHS	945:947	arg1	ester					949:953	the intermediate - NHS ester	926:953	the intermediate - NHS ester	926:953	NMR studies showed that EtOH inhibited grafting of GA by prohibiting the production of the intermediate - NHS ester.
26876841	4	28	theme	largest	709:715	arg1	extent					717:722	the largest extent	705:722	the largest extent (285.9mg GA/g chitosan)	705:746	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	4	28	theme	largest	709:715	arg1	chitosan					738:745	285.9mg GA/g chitosan	725:745	285.9mg GA/g chitosan	725:745	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	1	29	theme	primary	94:100	arg1	AOX					115:117	AOX	115:117	AOX	115:117	The primary antioxidant (AOX) activity of chitosan can be introduced by grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups.
26876841	1	29	theme	primary	94:100	arg1	antioxidant					102:112	primary antioxidant	94:112	The primary antioxidant (AOX) activity of chitosan	90:139	The primary antioxidant (AOX) activity of chitosan can be introduced by grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups.
26876841	1	30	theme	phenolic	174:181	arg1	compound					183:190	phenolic compound	174:190	phenolic compound	174:190	The primary antioxidant (AOX) activity of chitosan can be introduced by grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups.
26876841	4	31	theme	285.9mg	725:731	arg1	extent					717:722	the largest extent	705:722	the largest extent (285.9mg GA/g chitosan)	705:746	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	4	31	theme	285.9mg	725:731	arg1	chitosan					738:745	285.9mg GA/g chitosan	725:745	285.9mg GA/g chitosan	725:745	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	2	32	from	%	359:359	arg1	water					364:368	water	364:368	water	364:368	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	4	33	theme	sole	651:654	arg1	EtOH					668:671	0% EtOH	665:671	0% EtOH	665:671	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	4	33	theme	sole	651:654	arg1	water					631:635	pure deionized water	616:635	pure deionized water	616:635	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	4	33	theme	sole	651:654	arg1	solvent					656:662	a sole solvent	649:662	a sole solvent (0% EtOH)	649:672	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	1	34	theme	compound	183:190	arg1	grafting					162:169	grafting	162:169	grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups	162:245	The primary antioxidant (AOX) activity of chitosan can be introduced by grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups.
26876841	0	35	theme	composition	22:32	arg1	effect					4:9	The effect	0:9	The effect of solvent composition on	0:35	The effect of solvent composition on grafting gallic acid onto chitosan via carbodiimide.
26876841	2	36	theme	GA	397:398	arg1	efficiency					374:383	efficiency	374:383	efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS)	374:511	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	4	37	from	extent	717:722	arg1	chitosan					693:700	chitosan	693:700	chitosan at the largest extent (285.9mg GA/g chitosan)	693:746	When pure deionized water was used as a sole solvent (0% EtOH), GA was grafted to chitosan at the largest extent (285.9mg GA/g chitosan).
26876841	0	38	theme	gallic	46:51	arg1	acid					53:56	gallic acid	46:56	gallic acid onto chitosan	46:70	The effect of solvent composition on grafting gallic acid onto chitosan via carbodiimide.
26876841	2	39	theme	1-ethyl-3-	433:442	arg1	EDC					481:483	EDC	481:483	EDC	481:483	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	39	theme	1-ethyl-3-	433:442	arg1	-carbodiimide					466:478	1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide	433:478	1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS)	433:511	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	1	40	theme	antioxidant	102:112	arg1	activity					120:127	The primary antioxidant (AOX) activity	90:127	The primary antioxidant (AOX) activity of chitosan	90:139	The primary antioxidant (AOX) activity of chitosan can be introduced by grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups.
26876841	7	41	from	concentration	985:997	arg1	solution					1019:1026	grafting solution	1010:1026	grafting solution	1010:1026	The results confirm that the concentration of EtOH in grafting solution significantly affects grafting efficiency of GA on chitosan.
26876841	5	42	theme	EtOH	773:776	arg1	concentration					756:768	the concentration	752:768	the concentration of EtOH	752:776	As the concentration of EtOH increased, the grafting efficiency proportionally decreased.
26876841	1	43	theme	gallic	194:199	arg1	GA					207:208	GA	207:208	GA	207:208	The primary antioxidant (AOX) activity of chitosan can be introduced by grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups.
26876841	1	43	theme	gallic	194:199	arg1	acid					201:204	gallic acid	194:204	gallic acid (GA)	194:209	The primary antioxidant (AOX) activity of chitosan can be introduced by grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups.
26876841	2	44	theme	study	270:274	arg1	objective					252:260	The objective	248:260	The objective of this study	248:274	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	1	45	theme	acid	201:204	arg1	grafting					162:169	grafting	162:169	grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups	162:245	The primary antioxidant (AOX) activity of chitosan can be introduced by grafting of phenolic compound - gallic acid (GA) to its amino and/or hydroxyl groups.
26876841	7	46	theme	grafting	1010:1017	arg1	solution					1019:1026	grafting solution	1010:1026	grafting solution	1010:1026	The results confirm that the concentration of EtOH in grafting solution significantly affects grafting efficiency of GA on chitosan.
26876841	2	47	dep	concentration	324:336	arg1	%					359:359	75%	357:359	75%	357:359	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	47	dep	concentration	324:336	arg1	%					340:340	0%	339:340	0%	339:340	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	47	dep	concentration	324:336	arg1	%					345:345	25%	343:345	25%	343:345	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	47	dep	concentration	324:336	arg1	%					350:350	50%	348:350	50%	348:350	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	48	theme	ethanol	309:315	arg1	concentration					324:336	ethanol (EtOH) concentration	309:336	ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water)	309:369	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	49	from	%	345:345	arg1	water					364:368	water	364:368	water	364:368	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
26876841	2	50	from	chitosan	405:412	arg1	presence					421:428	the presence	417:428	the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS)	417:511	The objective of this study was to investigate the effect of ethanol (EtOH) concentration (0%, 25%, 50%, and 75% in water) on efficiency of grafting GA onto chitosan in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS).
24708976	0	0	theme	binary	76:81	arg1	solvents					83:90	binary solvents	76:90	binary solvents with ionic liquid	76:108	Phase diagram, solubility limit and hydrodynamic properties of cellulose in binary solvents with ionic liquid.
24708976	5	1	theme	solvent	762:768	arg1	systems					770:776	two solvent systems	758:776	two solvent systems	758:776	Hydrodynamic properties of cellulose in two solvent systems were compared.
24708976	0	2	from	diagram	6:12	arg1	solvents					83:90	binary solvents	76:90	binary solvents with ionic liquid	76:108	Phase diagram, solubility limit and hydrodynamic properties of cellulose in binary solvents with ionic liquid.
24708976	6	3	theme	intrinsic	818:826	arg1	viscosity					828:836	cellulose intrinsic viscosity	808:836	cellulose intrinsic viscosity	808:836	In EmimAc-DMSO cellulose intrinsic viscosity practically does not depend on DMSO content as predicted by the phase diagram.
24708976	1	4	theme	binary	154:159	arg1	solvents					161:168	two binary solvents	150:168	two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO)	150:273	Cellulose solubility phase diagrams in two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO) were built.
24708976	3	5	from	%	541:541	arg1	EmimAc					514:519	EmimAc	514:519	EmimAc	514:519	This proportion allows calculation of the maximal cellulose concentration soluble in EmimAc-DMSO at any composition; in EmimAc it is around 25-27wt%.
24708976	6	6	theme	phase	902:906	arg1	diagram					908:914	the phase diagram	898:914	the phase diagram	898:914	In EmimAc-DMSO cellulose intrinsic viscosity practically does not depend on DMSO content as predicted by the phase diagram.
24708976	7	7	theme	water	978:982	arg1	content					984:990	water content	978:990	water content due to cellulose self-aggregation	978:1024	The intrinsic viscosity in EmimAc-water first increases with water content due to cellulose self-aggregation and then abruptly decreases due to coagulation.
24708976	6	8	theme	cellulose	808:816	arg1	viscosity					828:836	cellulose intrinsic viscosity	808:836	cellulose intrinsic viscosity	808:836	In EmimAc-DMSO cellulose intrinsic viscosity practically does not depend on DMSO content as predicted by the phase diagram.
24708976	4	9	with	bonds	565:569	arg1	EmimAc					576:581	EmimAc	576:581	EmimAc	576:581	Water forms hydrogen bonds with EmimAc and thus competes with cellulose for ionic liquid; the solubility of cellulose in EmimAc-water is much lower than that in EmimAc-DMSO.
24708976	5	10	theme	Hydrodynamic	718:729	arg1	properties					731:740	Hydrodynamic properties	718:740	Hydrodynamic properties of cellulose in two solvent systems	718:776	Hydrodynamic properties of cellulose in two solvent systems were compared.
24708976	0	11	from	properties	49:58	arg1	solvents					83:90	binary solvents	76:90	binary solvents with ionic liquid	76:108	Phase diagram, solubility limit and hydrodynamic properties of cellulose in binary solvents with ionic liquid.
24708976	1	12	from	diagrams	138:145	arg1	solvents					161:168	two binary solvents	150:168	two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO)	150:273	Cellulose solubility phase diagrams in two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO) were built.
24708976	1	13	theme	phase	132:136	arg1	diagrams					138:145	Cellulose solubility phase diagrams	111:145	Cellulose solubility phase diagrams in two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO)	111:273	Cellulose solubility phase diagrams in two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO) were built.
24708976	3	14	theme	maximal	436:442	arg1	concentration					454:466	the maximal cellulose concentration	432:466	the maximal cellulose concentration soluble in EmimAc-DMSO	432:489	This proportion allows calculation of the maximal cellulose concentration soluble in EmimAc-DMSO at any composition; in EmimAc it is around 25-27wt%.
24708976	3	15	theme	cellulose	444:452	arg1	concentration					454:466	the maximal cellulose concentration	432:466	the maximal cellulose concentration soluble in EmimAc-DMSO	432:489	This proportion allows calculation of the maximal cellulose concentration soluble in EmimAc-DMSO at any composition; in EmimAc it is around 25-27wt%.
24708976	0	16	from	limit	26:30	arg1	solvents					83:90	binary solvents	76:90	binary solvents with ionic liquid	76:108	Phase diagram, solubility limit and hydrodynamic properties of cellulose in binary solvents with ionic liquid.
24708976	5	17	theme	cellulose	745:753	arg1	properties					731:740	Hydrodynamic properties	718:740	Hydrodynamic properties of cellulose in two solvent systems	718:776	Hydrodynamic properties of cellulose in two solvent systems were compared.
24708976	4	18	from	solubility	638:647	arg1	EmimAc-water					665:676	EmimAc-water	665:676	EmimAc-water	665:676	Water forms hydrogen bonds with EmimAc and thus competes with cellulose for ionic liquid; the solubility of cellulose in EmimAc-water is much lower than that in EmimAc-DMSO.
24708976	2	19	theme	anhydroglucose	373:386	arg1	unit					388:391	anhydroglucose unit	373:391	anhydroglucose unit	373:391	The minimal amount of EmimAc molecules needed to dissolve cellulose is 2.5-3moles per anhydroglucose unit.
24708976	0	20	theme	Phase	0:4	arg1	diagram					6:12	Phase diagram	0:12	Phase diagram	0:12	Phase diagram, solubility limit and hydrodynamic properties of cellulose in binary solvents with ionic liquid.
24708976	7	21	theme	intrinsic	921:929	arg1	viscosity					931:939	The intrinsic viscosity	917:939	The intrinsic viscosity in EmimAc-water	917:955	The intrinsic viscosity in EmimAc-water first increases with water content due to cellulose self-aggregation and then abruptly decreases due to coagulation.
24708976	1	22	theme	1-ethyl-3-methylimidazolium	179:205	arg1	EmimAc					216:221	EmimAc	216:221	EmimAc	216:221	Cellulose solubility phase diagrams in two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO) were built.
24708976	1	22	theme	1-ethyl-3-methylimidazolium	179:205	arg1	acetate					207:213	1-ethyl-3-methylimidazolium acetate	179:213	1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO)	179:273	Cellulose solubility phase diagrams in two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO) were built.
24708976	0	23	theme	solubility	15:24	arg1	limit					26:30	solubility limit	15:30	solubility limit	15:30	Phase diagram, solubility limit and hydrodynamic properties of cellulose in binary solvents with ionic liquid.
24708976	0	24	with	solvents	83:90	arg1	liquid					103:108	liquid	103:108	liquid	103:108	Phase diagram, solubility limit and hydrodynamic properties of cellulose in binary solvents with ionic liquid.
24708976	2	25	theme	minimal	291:297	arg1	amount					299:304	The minimal amount	287:304	The minimal amount of EmimAc molecules needed to dissolve cellulose	287:353	The minimal amount of EmimAc molecules needed to dissolve cellulose is 2.5-3moles per anhydroglucose unit.
24708976	2	25	theme	minimal	291:297	arg1	molecules					316:324	EmimAc molecules	309:324	EmimAc molecules	309:324	The minimal amount of EmimAc molecules needed to dissolve cellulose is 2.5-3moles per anhydroglucose unit.
24708976	2	25	theme	minimal	291:297	arg1	2.5-3moles					358:367	2.5-3moles	358:367	2.5-3moles per anhydroglucose unit	358:391	The minimal amount of EmimAc molecules needed to dissolve cellulose is 2.5-3moles per anhydroglucose unit.
24708976	5	26	from	properties	731:740	arg1	systems					770:776	two solvent systems	758:776	two solvent systems	758:776	Hydrodynamic properties of cellulose in two solvent systems were compared.
24708976	4	27	theme	hydrogen	556:563	arg1	bonds					565:569	hydrogen bonds	556:569	hydrogen bonds with EmimAc	556:581	Water forms hydrogen bonds with EmimAc and thus competes with cellulose for ionic liquid; the solubility of cellulose in EmimAc-water is much lower than that in EmimAc-DMSO.
24708976	6	28	theme	DMSO	869:872	arg1	content					874:880	DMSO content	869:880	DMSO content	869:880	In EmimAc-DMSO cellulose intrinsic viscosity practically does not depend on DMSO content as predicted by the phase diagram.
24708976	4	29	theme	cellulose	652:660	arg1	lower					686:690	lower	686:690	lower	686:690	Water forms hydrogen bonds with EmimAc and thus competes with cellulose for ionic liquid; the solubility of cellulose in EmimAc-water is much lower than that in EmimAc-DMSO.
24708976	4	29	theme	cellulose	652:660	arg1	solubility					638:647	the solubility	634:647	the solubility of cellulose in EmimAc-water	634:676	Water forms hydrogen bonds with EmimAc and thus competes with cellulose for ionic liquid; the solubility of cellulose in EmimAc-water is much lower than that in EmimAc-DMSO.
24708976	3	30	theme	concentration	454:466	arg1	calculation					417:427	calculation	417:427	calculation of the maximal cellulose concentration soluble in EmimAc-DMSO	417:489	This proportion allows calculation of the maximal cellulose concentration soluble in EmimAc-DMSO at any composition; in EmimAc it is around 25-27wt%.
24708976	7	31	from	viscosity	931:939	arg1	EmimAc-water					944:955	EmimAc-water	944:955	EmimAc-water	944:955	The intrinsic viscosity in EmimAc-water first increases with water content due to cellulose self-aggregation and then abruptly decreases due to coagulation.
24708976	3	32	theme	soluble	468:474	arg1	concentration					454:466	the maximal cellulose concentration	432:466	the maximal cellulose concentration soluble in EmimAc-DMSO	432:489	This proportion allows calculation of the maximal cellulose concentration soluble in EmimAc-DMSO at any composition; in EmimAc it is around 25-27wt%.
24708976	3	33	from	soluble	468:474	arg1	EmimAc-DMSO					479:489	EmimAc-DMSO	479:489	EmimAc-DMSO	479:489	This proportion allows calculation of the maximal cellulose concentration soluble in EmimAc-DMSO at any composition; in EmimAc it is around 25-27wt%.
24708976	0	34	theme	hydrodynamic	36:47	arg1	properties					49:58	hydrodynamic properties	36:58	hydrodynamic properties	36:58	Phase diagram, solubility limit and hydrodynamic properties of cellulose in binary solvents with ionic liquid.
24708976	1	35	theme	Cellulose	111:119	arg1	diagrams					138:145	Cellulose solubility phase diagrams	111:145	Cellulose solubility phase diagrams in two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO)	111:273	Cellulose solubility phase diagrams in two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO) were built.
24708976	3	36	from	EmimAc-DMSO	479:489	arg1	soluble					468:474	soluble	468:474	soluble	468:474	This proportion allows calculation of the maximal cellulose concentration soluble in EmimAc-DMSO at any composition; in EmimAc it is around 25-27wt%.
24708976	1	37	theme	solubility	121:130	arg1	diagrams					138:145	Cellulose solubility phase diagrams	111:145	Cellulose solubility phase diagrams in two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO)	111:273	Cellulose solubility phase diagrams in two binary solvents based on 1-ethyl-3-methylimidazolium acetate (EmimAc) mixed with water and with dimethylsulfoxide (DMSO) were built.
24708976	7	38	dep	increases	963:971	arg1	decreases					1044:1052	decreases	1044:1052	decreases due to coagulation	1044:1071	The intrinsic viscosity in EmimAc-water first increases with water content due to cellulose self-aggregation and then abruptly decreases due to coagulation.
24708976	2	39	theme	EmimAc	309:314	arg1	molecules					316:324	EmimAc molecules	309:324	EmimAc molecules	309:324	The minimal amount of EmimAc molecules needed to dissolve cellulose is 2.5-3moles per anhydroglucose unit.
24708976	2	40	theme	molecules	316:324	arg1	amount					299:304	The minimal amount	287:304	The minimal amount of EmimAc molecules needed to dissolve cellulose	287:353	The minimal amount of EmimAc molecules needed to dissolve cellulose is 2.5-3moles per anhydroglucose unit.
24708976	2	40	theme	molecules	316:324	arg1	molecules					316:324	EmimAc molecules	309:324	EmimAc molecules	309:324	The minimal amount of EmimAc molecules needed to dissolve cellulose is 2.5-3moles per anhydroglucose unit.
24708976	2	40	theme	molecules	316:324	arg1	2.5-3moles					358:367	2.5-3moles	358:367	2.5-3moles per anhydroglucose unit	358:391	The minimal amount of EmimAc molecules needed to dissolve cellulose is 2.5-3moles per anhydroglucose unit.
24708976	0	41	theme	cellulose	63:71	arg1	diagram					6:12	Phase diagram	0:12	Phase diagram	0:12	Phase diagram, solubility limit and hydrodynamic properties of cellulose in binary solvents with ionic liquid.
24708976	0	41	theme	cellulose	63:71	arg1	limit					26:30	solubility limit	15:30	solubility limit	15:30	Phase diagram, solubility limit and hydrodynamic properties of cellulose in binary solvents with ionic liquid.
24708976	0	41	theme	cellulose	63:71	arg1	properties					49:58	hydrodynamic properties	36:58	hydrodynamic properties	36:58	Phase diagram, solubility limit and hydrodynamic properties of cellulose in binary solvents with ionic liquid.
24708976	7	42	theme	cellulose	999:1007	arg1	self-aggregation					1009:1024	cellulose self-aggregation	999:1024	cellulose self-aggregation	999:1024	The intrinsic viscosity in EmimAc-water first increases with water content due to cellulose self-aggregation and then abruptly decreases due to coagulation.
27242044	10	0	theme	Part	1261:1264	arg1	A					1266:1266	Part A	1261:1266	J Biomed Mater Res Part A: 104A: 2576-2584, 2016.	1242:1290	J Biomed Mater Res Part A: 104A: 2576-2584, 2016.
27242044	5	1	from	BC	727:728	arg1	concentration					739:751	small concentration	733:751	small concentration	733:751	BC in small concentration determined an increase in the mechanical properties due to a concerted action of PHA and filler.
27242044	0	2	theme	in	103:104	arg1	composites					56:65	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites	0:65	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.	0:122	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	2	theme	in	103:104	arg1	evaluation					112:121	in vitro evaluation	103:121	in vitro evaluation	103:121	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	10	3	dep	Res	1257:1259	arg1	104A					1269:1272	104A	1269:1272	104A	1269:1272	J Biomed Mater Res Part A: 104A: 2576-2584, 2016.
27242044	10	3	dep	Res	1257:1259	arg1	A					1266:1266	Part A	1261:1266	J Biomed Mater Res Part A: 104A: 2576-2584, 2016.	1242:1290	J Biomed Mater Res Part A: 104A: 2576-2584, 2016.
27242044	3	4	theme	polymer	525:531	arg1	matrix					533:538	the polymer matrix	521:538	the polymer matrix	521:538	BC addition increased the thermal stability of the polymer matrix as evidenced by thermogravimetric analysis.
27242044	9	5	dep	©	1211:1211	arg1	Inc.					1237:1240	Inc.	1237:1240	Inc.	1237:1240	© 2016 Wiley Periodicals, Inc.
27242044	4	6	theme	PHB	605:607	arg1	crystallinity					588:600	The crystallinity	584:600	The crystallinity of PHB	584:607	The crystallinity of PHB and the crystallization temperature decreased with the addition of BC and PHA, thus increasing the processing window.
27242044	4	6	theme	PHB	605:607	arg1	temperature					633:643	the crystallization temperature	613:643	the crystallization temperature	613:643	The crystallinity of PHB and the crystallization temperature decreased with the addition of BC and PHA, thus increasing the processing window.
27242044	2	7	theme	bacterial	298:306	arg1	BC					319:320	BC	319:320	BC	319:320	Novel biocomposites made from poly(3-hydroxybutyrate) (PHB), polyhydroxyalkanoate (PHA) and bacterial cellulose (BC) in different concentrations were prepared by solution casting and their thermal and mechanical behavior as well as biocompatibility was characterized.
27242044	2	7	theme	bacterial	298:306	arg1	cellulose					308:316	bacterial cellulose	298:316	bacterial cellulose (BC)	298:321	Novel biocomposites made from poly(3-hydroxybutyrate) (PHB), polyhydroxyalkanoate (PHA) and bacterial cellulose (BC) in different concentrations were prepared by solution casting and their thermal and mechanical behavior as well as biocompatibility was characterized.
27242044	4	8	theme	crystallization	617:631	arg1	temperature					633:643	the crystallization temperature	613:643	the crystallization temperature	613:643	The crystallinity of PHB and the crystallization temperature decreased with the addition of BC and PHA, thus increasing the processing window.
27242044	10	9	dep	104A	1269:1272	arg1	2576-2584					1275:1283	2576-2584	1275:1283	2576-2584	1275:1283	J Biomed Mater Res Part A: 104A: 2576-2584, 2016.
27242044	7	10	theme	hydrophobic	960:970	arg1	PHA					950:952	PHA	950:952	PHA (more hydrophobic than the matrix)	950:987	By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled.
27242044	8	11	theme	biomedical	1186:1195	arg1	applications					1197:1208	biomedical applications	1186:1208	biomedical applications	1186:1208	Cytocompatibility studies using L929 cell line revealed that this material is suitable for biomedical applications.
27242044	1	12	from	scaffolds	173:181	arg1	engineering					193:203	tissue engineering	186:203	tissue engineering	186:203	Biocompatible composites play a critical role as scaffolds in tissue engineering.
27242044	5	13	theme	PHA	834:836	arg1	action					824:829	a concerted action	812:829	a concerted action of PHA and filler	812:847	BC in small concentration determined an increase in the mechanical properties due to a concerted action of PHA and filler.
27242044	2	14	theme	thermal	395:401	arg1	behavior					418:425	their thermal and mechanical behavior	389:425	their thermal and mechanical behavior as well as biocompatibility	389:453	Novel biocomposites made from poly(3-hydroxybutyrate) (PHB), polyhydroxyalkanoate (PHA) and bacterial cellulose (BC) in different concentrations were prepared by solution casting and their thermal and mechanical behavior as well as biocompatibility was characterized.
27242044	0	15	theme	polyhydroxyalkanoates/bacterial	14:44	arg1	Preparation					68:78	Preparation	68:78	Preparation	68:78	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	15	theme	polyhydroxyalkanoates/bacterial	14:44	arg1	composites					56:65	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites	0:65	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.	0:122	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	15	theme	polyhydroxyalkanoates/bacterial	14:44	arg1	evaluation					112:121	in vitro evaluation	103:121	in vitro evaluation	103:121	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	15	theme	polyhydroxyalkanoates/bacterial	14:44	arg1	characterization					81:96	characterization	81:96	characterization	81:96	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	5	16	theme	due	805:807	arg1	properties					794:803	the mechanical properties	779:803	the mechanical properties due to a concerted action of PHA and filler	779:847	BC in small concentration determined an increase in the mechanical properties due to a concerted action of PHA and filler.
27242044	8	17	theme	Cytocompatibility	1095:1111	arg1	studies					1113:1119	Cytocompatibility studies	1095:1119	Cytocompatibility studies using L929 cell line	1095:1140	Cytocompatibility studies using L929 cell line revealed that this material is suitable for biomedical applications.
27242044	0	18	theme	Biocompatible	0:12	arg1	Preparation					68:78	Preparation	68:78	Preparation	68:78	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	18	theme	Biocompatible	0:12	arg1	composites					56:65	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites	0:65	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.	0:122	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	18	theme	Biocompatible	0:12	arg1	evaluation					112:121	in vitro evaluation	103:121	in vitro evaluation	103:121	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	18	theme	Biocompatible	0:12	arg1	characterization					81:96	characterization	81:96	characterization	81:96	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	19	dep	in	103:104	arg1	vitro					106:110	vitro	106:110	vitro	106:110	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	7	20	theme	cell	1060:1063	arg1	attachment					1065:1074	cell attachment	1060:1074	cell attachment	1060:1074	By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled.
27242044	7	21	theme	PHA	950:952	arg1	addition					938:945	the addition	934:945	the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic)	934:1034	By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled.
27242044	1	22	theme	Biocompatible	124:136	arg1	composites					138:147	Biocompatible composites	124:147	Biocompatible composites	124:147	Biocompatible composites play a critical role as scaffolds in tissue engineering.
27242044	0	23	theme	cellulose	46:54	arg1	Preparation					68:78	Preparation	68:78	Preparation	68:78	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	23	theme	cellulose	46:54	arg1	composites					56:65	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites	0:65	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.	0:122	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	23	theme	cellulose	46:54	arg1	evaluation					112:121	in vitro evaluation	103:121	in vitro evaluation	103:121	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	23	theme	cellulose	46:54	arg1	characterization					81:96	characterization	81:96	characterization	81:96	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	5	24	from	increase	767:774	arg1	properties					794:803	the mechanical properties	779:803	the mechanical properties due to a concerted action of PHA and filler	779:847	BC in small concentration determined an increase in the mechanical properties due to a concerted action of PHA and filler.
27242044	1	25	theme	tissue	186:191	arg1	engineering					193:203	tissue engineering	186:203	tissue engineering	186:203	Biocompatible composites play a critical role as scaffolds in tissue engineering.
27242044	3	26	theme	matrix	533:538	arg1	stability					508:516	the thermal stability	496:516	the thermal stability of the polymer matrix	496:538	BC addition increased the thermal stability of the polymer matrix as evidenced by thermogravimetric analysis.
27242044	6	27	dep	cells	855:859	arg1	cells					855:859	Good cells	850:859	Good cells attachment and proliferation	850:888	Good cells attachment and proliferation were observed for all the biocomposites.
27242044	6	27	dep	cells	855:859	arg1	proliferation					876:888	proliferation	876:888	proliferation	876:888	Good cells attachment and proliferation were observed for all the biocomposites.
27242044	6	27	dep	cells	855:859	arg1	attachment					861:870	attachment	861:870	attachment	861:870	Good cells attachment and proliferation were observed for all the biocomposites.
27242044	5	28	theme	small	733:737	arg1	concentration					739:751	small concentration	733:751	small concentration	733:751	BC in small concentration determined an increase in the mechanical properties due to a concerted action of PHA and filler.
27242044	2	29	theme	Novel	206:210	arg1	biocomposites					212:224	Novel biocomposites	206:224	Novel biocomposites made from poly(3-hydroxybutyrate) (PHB), polyhydroxyalkanoate (PHA) and bacterial cellulose (BC) in different concentrations	206:349	Novel biocomposites made from poly(3-hydroxybutyrate) (PHB), polyhydroxyalkanoate (PHA) and bacterial cellulose (BC) in different concentrations were prepared by solution casting and their thermal and mechanical behavior as well as biocompatibility was characterized.
27242044	4	30	theme	BC	676:677	arg1	addition					664:671	the addition	660:671	the addition of BC and PHA	660:685	The crystallinity of PHB and the crystallization temperature decreased with the addition of BC and PHA, thus increasing the processing window.
27242044	3	31	theme	BC	474:475	arg1	addition					477:484	BC addition	474:484	BC addition	474:484	BC addition increased the thermal stability of the polymer matrix as evidenced by thermogravimetric analysis.
27242044	5	32	theme	concerted	814:822	arg1	action					824:829	a concerted action	812:829	a concerted action of PHA and filler	812:847	BC in small concentration determined an increase in the mechanical properties due to a concerted action of PHA and filler.
27242044	8	33	theme	cell	1132:1135	arg1	line					1137:1140	L929 cell line	1127:1140	L929 cell line	1127:1140	Cytocompatibility studies using L929 cell line revealed that this material is suitable for biomedical applications.
27242044	8	34	theme	L929	1127:1130	arg1	line					1137:1140	L929 cell line	1127:1140	L929 cell line	1127:1140	Cytocompatibility studies using L929 cell line revealed that this material is suitable for biomedical applications.
27242044	7	35	theme	surface	1037:1043	arg1	properties					1045:1054	surface properties	1037:1054	surface properties	1037:1054	By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled.
27242044	7	36	theme	hydrophilic	1023:1033	arg1	PHA					950:952	PHA	950:952	PHA (more hydrophobic than the matrix)	950:987	By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled.
27242044	0	37	dep	composites	56:65	arg1	Preparation					68:78	Preparation	68:78	Preparation	68:78	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	37	dep	composites	56:65	arg1	composites					56:65	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites	0:65	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.	0:122	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	37	dep	composites	56:65	arg1	evaluation					112:121	in vitro evaluation	103:121	in vitro evaluation	103:121	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	0	37	dep	composites	56:65	arg1	characterization					81:96	characterization	81:96	characterization	81:96	Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
27242044	7	38	theme	BC	1012:1013	arg1	BC					1012:1013	BC	1012:1013	BC	1012:1013	By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled.
27242044	7	38	theme	BC	1012:1013	arg1	amounts					1001:1007	various amounts	993:1007	various amounts of BC	993:1013	By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled.
27242044	7	38	theme	BC	1012:1013	arg1	PHA					950:952	PHA	950:952	PHA (more hydrophobic than the matrix)	950:987	By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled.
27242044	1	39	theme	critical	156:163	arg1	scaffolds					173:181	scaffolds	173:181	scaffolds in tissue engineering	173:203	Biocompatible composites play a critical role as scaffolds in tissue engineering.
27242044	1	39	theme	critical	156:163	arg1	role					165:168	a critical role	154:168	a critical role	154:168	Biocompatible composites play a critical role as scaffolds in tissue engineering.
27242044	6	40	theme	Good	850:853	arg1	cells					855:859	Good cells	850:859	Good cells attachment and proliferation	850:888	Good cells attachment and proliferation were observed for all the biocomposites.
27242044	6	40	theme	Good	850:853	arg1	proliferation					876:888	proliferation	876:888	proliferation	876:888	Good cells attachment and proliferation were observed for all the biocomposites.
27242044	6	40	theme	Good	850:853	arg1	attachment					861:870	attachment	861:870	attachment	861:870	Good cells attachment and proliferation were observed for all the biocomposites.
27242044	3	41	theme	thermogravimetric	556:572	arg1	analysis					574:581	thermogravimetric analysis	556:581	thermogravimetric analysis	556:581	BC addition increased the thermal stability of the polymer matrix as evidenced by thermogravimetric analysis.
27242044	7	42	theme	various	993:999	arg1	BC					1012:1013	BC	1012:1013	BC	1012:1013	By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled.
27242044	7	42	theme	various	993:999	arg1	amounts					1001:1007	various amounts	993:1007	various amounts of BC	993:1013	By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled.
27242044	3	43	theme	thermal	500:506	arg1	stability					508:516	the thermal stability	496:516	the thermal stability of the polymer matrix	496:538	BC addition increased the thermal stability of the polymer matrix as evidenced by thermogravimetric analysis.
27242044	2	44	theme	different	326:334	arg1	concentrations					336:349	different concentrations	326:349	different concentrations	326:349	Novel biocomposites made from poly(3-hydroxybutyrate) (PHB), polyhydroxyalkanoate (PHA) and bacterial cellulose (BC) in different concentrations were prepared by solution casting and their thermal and mechanical behavior as well as biocompatibility was characterized.
27242044	2	45	theme	mechanical	407:416	arg1	behavior					418:425	their thermal and mechanical behavior	389:425	their thermal and mechanical behavior as well as biocompatibility	389:453	Novel biocomposites made from poly(3-hydroxybutyrate) (PHB), polyhydroxyalkanoate (PHA) and bacterial cellulose (BC) in different concentrations were prepared by solution casting and their thermal and mechanical behavior as well as biocompatibility was characterized.
27242044	7	46	theme	amounts	1001:1007	arg1	addition					938:945	the addition	934:945	the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic)	934:1034	By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled.
27242044	4	47	theme	processing	708:717	arg1	window					719:724	the processing window	704:724	the processing window	704:724	The crystallinity of PHB and the crystallization temperature decreased with the addition of BC and PHA, thus increasing the processing window.
27242044	4	48	theme	PHA	683:685	arg1	addition					664:671	the addition	660:671	the addition of BC and PHA	660:685	The crystallinity of PHB and the crystallization temperature decreased with the addition of BC and PHA, thus increasing the processing window.
27242044	5	49	theme	mechanical	783:792	arg1	properties					794:803	the mechanical properties	779:803	the mechanical properties due to a concerted action of PHA and filler	779:847	BC in small concentration determined an increase in the mechanical properties due to a concerted action of PHA and filler.
27242044	5	50	theme	filler	842:847	arg1	action					824:829	a concerted action	812:829	a concerted action of PHA and filler	812:847	BC in small concentration determined an increase in the mechanical properties due to a concerted action of PHA and filler.
27242044	2	51	theme	solution	368:375	arg1	casting					377:383	solution casting	368:383	solution casting	368:383	Novel biocomposites made from poly(3-hydroxybutyrate) (PHB), polyhydroxyalkanoate (PHA) and bacterial cellulose (BC) in different concentrations were prepared by solution casting and their thermal and mechanical behavior as well as biocompatibility was characterized.
27029113	5	0	theme	mass-spectrometric	1080:1097	arg1	analysis					1099:1106	mass-spectrometric analysis	1080:1106	mass-spectrometric analysis	1080:1106	CONCLUSION Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
27029113	1	1	theme	Brucella	361:368	arg1	agents					323:328	brucellosis causative agents	301:328	brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA)	301:382	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	1	theme	Brucella	361:368	arg1	19BA					378:381	Brucella abortus 19BA	361:381	Brucella abortus 19BA	361:381	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	2	dep	Brucella	331:338	arg1	melitensis					340:349	melitensis	340:349	melitensis	340:349	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	5	3	theme	data	975:978	arg1	analysis					959:966	the analysis	955:966	the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis	955:1106	CONCLUSION Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
27029113	0	4	theme	AGENT	85:89	arg1	STRAINS					52:58	STRAINS	52:58	STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE	52:119	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
27029113	4	5	theme	nutrient	913:920	arg1	medium					922:927	the nutrient medium	909:927	the nutrient medium used	909:932	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	5	6	theme	agar	1004:1007	arg1	data					975:978	the data	971:978	the data obtained	971:987	CONCLUSION Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
27029113	5	6	theme	agar	1004:1007	arg1	use					990:992	use	990:992	use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis	990:1106	CONCLUSION Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
27029113	5	7	theme	samples	1068:1074	arg1	preparation					1036:1046	preparation	1036:1046	preparation of brucella culture samples for mass-spectrometric analysis	1036:1106	CONCLUSION Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
27029113	0	8	from	[FEATURES	0:8	arg1	MEDIA					141:145	VARIOUS NUTRIENT MEDIA	124:145	VARIOUS NUTRIENT MEDIA	124:145	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
27029113	3	9	theme	Protein	559:565	arg1	profiling					567:575	Protein profiling	559:575	Protein profiling in linear mode on Microflex	559:603	Protein profiling in linear mode on Microflex "Bruker Daltonics" MALDI-TOF mass-spectrometer.
27029113	3	10	from	profiling	567:575	arg1	Microflex					595:603	Microflex	595:603	Microflex	595:603	Protein profiling in linear mode on Microflex "Bruker Daltonics" MALDI-TOF mass-spectrometer.
27029113	3	10	from	profiling	567:575	arg1	mode					587:590	linear mode	580:590	linear mode	580:590	Protein profiling in linear mode on Microflex "Bruker Daltonics" MALDI-TOF mass-spectrometer.
27029113	1	11	theme	protein	281:287	arg1	agar					430:433	Albimi agar	423:433	Albimi agar	423:433	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	11	theme	protein	281:287	arg1	brucellagar					436:446	brucellagar	436:446	brucellagar	436:446	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	11	theme	protein	281:287	arg1	erythrit-agar					452:464	erythrit-agar	452:464	erythrit-agar	452:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	11	theme	protein	281:287	arg1	profiles					289:296	protein profiles	281:296	protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	281:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	2	12	theme	Brucella	536:543	arg1	19BA					553:556	Brucella abortus 19BA	536:556	Brucella abortus 19BA	536:556	MATERIALS AND METHODS Vaccine,strains: Brucella melitensis Rev-1 and Brucella abortus 19BA.
27029113	2	13	dep	Vaccine	489:495	arg1	19BA					553:556	Brucella abortus 19BA	536:556	Brucella abortus 19BA	536:556	MATERIALS AND METHODS Vaccine,strains: Brucella melitensis Rev-1 and Brucella abortus 19BA.
27029113	2	13	dep	Vaccine	489:495	arg1	Rev-1					526:530	Brucella melitensis Rev-1	506:530	Brucella melitensis Rev-1	506:530	MATERIALS AND METHODS Vaccine,strains: Brucella melitensis Rev-1 and Brucella abortus 19BA.
27029113	4	14	theme	composition	789:799	arg1	differences					849:859	significant differences	837:859	significant differences in the intensity of separate peaks	837:894	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	4	14	theme	composition	789:799	arg1	preservation					751:762	preservation	751:762	preservation of the total qualitative composition of protein profiles of cultures	751:831	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	1	15	theme	profiles	289:296	arg1	desorption/ionization					241:261	matrix laser desorption/ionization	228:261	matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	228:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	15	theme	profiles	289:296	arg1	MS					274:275	MALDI-TOF MS	264:275	MALDI-TOF MS	264:275	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	16	contain	Carry	153:157	arg1	AIM					149:151	AIM	149:151	AIM	149:151	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	16	contain	Carry	153:157	arg2	analysis					175:182	comparative analysis	163:182	comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	163:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	4	17	theme	separate	881:888	arg1	peaks					890:894	separate peaks	881:894	separate peaks	881:894	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	4	18	theme	profiles	812:819	arg1	composition					789:799	the total qualitative composition	767:799	the total qualitative composition of protein profiles of cultures	767:831	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	1	19	theme	comparative	163:173	arg1	analysis					175:182	comparative analysis	163:182	comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	163:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	0	20	theme	CULTURE	113:119	arg1	PREPARATION					98:108	PREPARATION	98:108	PREPARATION OF CULTURE	98:119	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
27029113	4	21	theme	protein	804:810	arg1	profiles					812:819	protein profiles	804:819	protein profiles of cultures	804:831	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	1	22	dep	agents	323:328	arg1	agents					323:328	brucellosis causative agents	301:328	brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA)	301:382	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	22	dep	agents	323:328	arg1	19BA					378:381	Brucella abortus 19BA	361:381	Brucella abortus 19BA	361:381	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	22	dep	agents	323:328	arg1	Rev-1					351:355	Brucella melitensis Rev-1	331:355	Brucella melitensis Rev-1	331:355	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	4	23	theme	characteristic	673:686	arg1	features					688:695	characteristic features	673:695	characteristic features of brucella mass-spectra	673:720	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	1	24	dep	Brucella	361:368	arg1	abortus					370:376	abortus	370:376	abortus	370:376	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	2	25	dep	MATERIALS	467:475	arg1	strains					497:503	strains	497:503	strains	497:503	MATERIALS AND METHODS Vaccine,strains: Brucella melitensis Rev-1 and Brucella abortus 19BA.
27029113	2	25	dep	MATERIALS	467:475	arg1	Vaccine					489:495	Vaccine	489:495	Vaccine	489:495	MATERIALS AND METHODS Vaccine,strains: Brucella melitensis Rev-1 and Brucella abortus 19BA.
27029113	5	26	theme	nutrient	1016:1023	arg1	medium					1025:1030	the nutrient medium	1012:1030	the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis	1012:1106	CONCLUSION Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
27029113	4	27	theme	brucella	700:707	arg1	mass-spectra					709:720	brucella mass-spectra	700:720	brucella mass-spectra	700:720	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	4	28	theme	peaks	890:894	arg1	intensity					868:876	the intensity	864:876	the intensity of separate peaks	864:894	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	4	29	theme	features	688:695	arg1	number					663:668	A number	661:668	A number of characteristic features of brucella mass-spectra	661:720	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	5	30	theme	culture	1060:1066	arg1	samples					1068:1074	brucella culture samples	1051:1074	brucella culture samples	1051:1074	CONCLUSION Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
27029113	1	31	theme	time-of-flight	190:203	arg1	mass-spectrometry					205:221	time-of-flight mass-spectrometry	190:221	time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	190:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	0	32	theme	PROTEIN	32:38	arg1	PROFILES					40:47	MASS-SPECTROMETRIC PROTEIN PROFILES	13:47	MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE	13:119	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
27029113	1	33	theme	brucellosis	301:311	arg1	agents					323:328	brucellosis causative agents	301:328	brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA)	301:382	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	33	theme	brucellosis	301:311	arg1	19BA					378:381	Brucella abortus 19BA	361:381	Brucella abortus 19BA	361:381	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	33	theme	brucellosis	301:311	arg1	Rev-1					351:355	Brucella melitensis Rev-1	331:355	Brucella melitensis Rev-1	331:355	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	0	34	theme	NUTRIENT	132:139	arg1	MEDIA					141:145	VARIOUS NUTRIENT MEDIA	124:145	VARIOUS NUTRIENT MEDIA	124:145	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
27029113	5	35	theme	brucella	1051:1058	arg1	samples					1068:1074	brucella culture samples	1051:1074	brucella culture samples	1051:1074	CONCLUSION Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
27029113	4	36	theme	qualitative	777:787	arg1	composition					789:799	the total qualitative composition	767:799	the total qualitative composition of protein profiles of cultures	767:831	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	0	37	theme	MASS-SPECTROMETRIC	13:30	arg1	PROFILES					40:47	MASS-SPECTROMETRIC PROTEIN PROFILES	13:47	MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE	13:119	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
27029113	1	38	theme	causative	313:321	arg1	agents					323:328	brucellosis causative agents	301:328	brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA)	301:382	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	38	theme	causative	313:321	arg1	19BA					378:381	Brucella abortus 19BA	361:381	Brucella abortus 19BA	361:381	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	38	theme	causative	313:321	arg1	Rev-1					351:355	Brucella melitensis Rev-1	331:355	Brucella melitensis Rev-1	331:355	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	0	39	theme	VARIOUS	124:130	arg1	MEDIA					141:145	VARIOUS NUTRIENT MEDIA	124:145	VARIOUS NUTRIENT MEDIA	124:145	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
27029113	1	40	theme	various	399:405	arg1	media					416:420	various nutrient media	399:420	various nutrient media	399:420	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	3	41	theme	linear	580:585	arg1	mode					587:590	linear mode	580:590	linear mode	580:590	Protein profiling in linear mode on Microflex "Bruker Daltonics" MALDI-TOF mass-spectrometer.
27029113	1	42	theme	agents	323:328	arg1	agar					430:433	Albimi agar	423:433	Albimi agar	423:433	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	42	theme	agents	323:328	arg1	brucellagar					436:446	brucellagar	436:446	brucellagar	436:446	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	42	theme	agents	323:328	arg1	erythrit-agar					452:464	erythrit-agar	452:464	erythrit-agar	452:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	42	theme	agents	323:328	arg1	profiles					289:296	protein profiles	281:296	protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	281:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	43	with	mass-spectrometry	205:221	arg1	desorption/ionization					241:261	matrix laser desorption/ionization	228:261	matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	228:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	43	with	mass-spectrometry	205:221	arg1	MS					274:275	MALDI-TOF MS	264:275	MALDI-TOF MS	264:275	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	44	theme	nutrient	407:414	arg1	media					416:420	various nutrient media	399:420	various nutrient media	399:420	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	3	45	dep	Daltonics	613:621	arg1	profiling					567:575	Protein profiling	559:575	Protein profiling in linear mode on Microflex	559:603	Protein profiling in linear mode on Microflex "Bruker Daltonics" MALDI-TOF mass-spectrometer.
27029113	1	46	theme	matrix	228:233	arg1	desorption/ionization					241:261	matrix laser desorption/ionization	228:261	matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	228:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	46	theme	matrix	228:233	arg1	MS					274:275	MALDI-TOF MS	264:275	MALDI-TOF MS	264:275	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	0	47	theme	PROFILES	40:47	arg1	[FEATURES					0:8	[FEATURES	0:8	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA	0:145	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
27029113	2	48	dep	Brucella	536:543	arg1	abortus					545:551	abortus	545:551	abortus	545:551	MATERIALS AND METHODS Vaccine,strains: Brucella melitensis Rev-1 and Brucella abortus 19BA.
27029113	1	49	theme	laser	235:239	arg1	desorption/ionization					241:261	matrix laser desorption/ionization	228:261	matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	228:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	49	theme	laser	235:239	arg1	MS					274:275	MALDI-TOF MS	264:275	MALDI-TOF MS	264:275	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	50	theme	Brucella	331:338	arg1	agents					323:328	brucellosis causative agents	301:328	brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA)	301:382	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	50	theme	Brucella	331:338	arg1	Rev-1					351:355	Brucella melitensis Rev-1	331:355	Brucella melitensis Rev-1	331:355	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	2	51	theme	Brucella	506:513	arg1	Rev-1					526:530	Brucella melitensis Rev-1	506:530	Brucella melitensis Rev-1	506:530	MATERIALS AND METHODS Vaccine,strains: Brucella melitensis Rev-1 and Brucella abortus 19BA.
27029113	0	52	theme	STRAINS	52:58	arg1	PROFILES					40:47	MASS-SPECTROMETRIC PROTEIN PROFILES	13:47	MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE	13:119	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
27029113	1	53	theme	Albimi	423:428	arg1	agar					430:433	Albimi agar	423:433	Albimi agar	423:433	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	53	theme	Albimi	423:428	arg1	profiles					289:296	protein profiles	281:296	protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	281:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	4	54	dep	RESULTS	653:659	arg1	detected					726:733	detected	726:733	was detected	722:733	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	4	54	dep	RESULTS	653:659	arg1	depending					896:904	depending	896:904	depending on the nutrient medium used	896:932	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	4	55	from	preservation	751:762	arg1	intensity					868:876	the intensity	864:876	the intensity of separate peaks	864:894	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	1	56	dep	profiles	289:296	arg1	agar					430:433	Albimi agar	423:433	Albimi agar	423:433	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	56	dep	profiles	289:296	arg1	profiles					289:296	protein profiles	281:296	protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	281:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	56	dep	profiles	289:296	arg1	erythrit-agar					452:464	erythrit-agar	452:464	erythrit-agar	452:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	56	dep	profiles	289:296	arg1	brucellagar					436:446	brucellagar	436:446	brucellagar	436:446	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	4	57	theme	cultures	824:831	arg1	profiles					812:819	protein profiles	804:819	protein profiles of cultures	804:831	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	4	58	from	differences	849:859	arg1	intensity					868:876	the intensity	864:876	the intensity of separate peaks	864:894	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	2	59	dep	Brucella	506:513	arg1	melitensis					515:524	melitensis	515:524	melitensis	515:524	MATERIALS AND METHODS Vaccine,strains: Brucella melitensis Rev-1 and Brucella abortus 19BA.
27029113	4	60	theme	total	771:775	arg1	composition					789:799	the total qualitative composition	767:799	the total qualitative composition of protein profiles of cultures	767:831	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	0	61	theme	CAUSATIVE	75:83	arg1	AGENT					85:89	BRUCELLOSIS CAUSATIVE AGENT	63:89	BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE	63:119	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
27029113	5	62	theme	Albimi	997:1002	arg1	agar					1004:1007	Albimi agar	997:1007	Albimi agar	997:1007	CONCLUSION Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
27029113	4	63	theme	significant	837:847	arg1	differences					849:859	significant differences	837:859	significant differences in the intensity of separate peaks	837:894	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
27029113	1	64	theme	MALDI-TOF	264:272	arg1	desorption/ionization					241:261	matrix laser desorption/ionization	228:261	matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar	228:464	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	1	64	theme	MALDI-TOF	264:272	arg1	MS					274:275	MALDI-TOF MS	264:275	MALDI-TOF MS	264:275	AIM Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar.
27029113	0	65	theme	BRUCELLOSIS	63:73	arg1	AGENT					85:89	BRUCELLOSIS CAUSATIVE AGENT	63:89	BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE	63:119	[FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
27029113	3	66	theme	MALDI-TOF	624:632	arg1	mass-spectrometer					634:650	MALDI-TOF mass-spectrometer	624:650	MALDI-TOF mass-spectrometer	624:650	Protein profiling in linear mode on Microflex "Bruker Daltonics" MALDI-TOF mass-spectrometer.
27029113	4	67	theme	mass-spectra	709:720	arg1	features					688:695	characteristic features	673:695	characteristic features of brucella mass-spectra	673:720	RESULTS A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used.
24747380	9	0	theme	packaging	1273:1281	arg1	purposes					1283:1290	food packaging purposes	1268:1290	food packaging purposes	1268:1290	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	4	1	from	solubility	492:501	arg1	permeability					586:597	oxygen permeability	579:597	oxygen permeability	579:597	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	1	from	solubility	492:501	arg1	capacity					530:537	water absorption capacity	513:537	water absorption capacity (WAC)	513:543	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	1	from	solubility	492:501	arg1	permeability					559:570	water vapour permeability	546:570	water vapour permeability (WVP)	546:576	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	1	from	solubility	492:501	arg1	WAC					540:542	WAC	540:542	WAC	540:542	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	1	from	solubility	492:501	arg1	WVP					573:575	WVP	573:575	WVP	573:575	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	1	from	solubility	492:501	arg1	water					506:510	water	506:510	water	506:510	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	1	from	solubility	492:501	arg1	properties					615:624	mechanical properties	604:624	mechanical properties of the films	604:637	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	1	2	theme	starch	162:167	arg1	films					193:197	potato starch based bio-nanocomposite films	155:197	potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials	155:251	In this research casting method was used to prepare potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials.
24747380	5	3	theme	mechanical	719:728	arg1	properties					730:739	mechanical properties	719:739	mechanical properties of films	719:748	Results showed that by increasing the concentration of nanoclay, mechanical properties of films were improved.
24747380	1	4	theme	based	169:173	arg1	films					193:197	potato starch based bio-nanocomposite films	155:197	potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials	155:251	In this research casting method was used to prepare potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials.
24747380	8	5	theme	molecules	1081:1089	arg1	permeability					1053:1064	permeability	1053:1064	permeability of the gaseous molecules	1053:1089	Also incorporation of clay nanoparticles in the structure of biopolymer decreased permeability of the gaseous molecules.
24747380	9	6	theme	nanoclay	1127:1134	arg1	addition					1104:1111	addition	1104:1111	addition	1104:1111	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	6	7	theme	Tensile	765:771	arg1	strength					773:780	Tensile strength	765:780	Tensile strength	765:780	Tensile strength was increased from 7.33 to 9.82MPa, and elongation at break decreased from 68.0 to 44.0%.
24747380	4	8	theme	mechanical	604:613	arg1	properties					615:624	mechanical properties	604:624	mechanical properties of the films	604:637	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	2	9	with	mixture	313:319	arg1	nanoclay					386:393	nanoclay	386:393	nanoclay (0-5% w/w)	386:404	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	2	9	with	mixture	313:319	arg1	%					399:399	0-5% w/w	396:403	0-5% w/w	396:403	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	2	10	theme	mixture	313:319	arg1	w/w					303:305	w/w	303:305	w/w	303:305	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	2	10	theme	mixture	313:319	arg1	%					300:300	40%	298:300	40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w)	298:404	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	2	10	theme	mixture	313:319	arg1	mixture					313:319	a mixture	311:319	a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w)	311:404	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	2	11	dep	ratio	350:354	arg1	plasticizer					368:378	plasticizer	368:378	weight ratio of 3 to 1as plasticizer	343:378	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	4	12	theme	water	546:550	arg1	WVP					573:575	WVP	573:575	WVP	573:575	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	12	theme	water	546:550	arg1	permeability					559:570	water vapour permeability	546:570	water vapour permeability (WVP)	546:576	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	9	13	theme	high	1238:1241	arg1	potential					1243:1251	high potential	1238:1251	high potential to be used for food packaging purposes	1238:1290	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	1	14	theme	bio-nanocomposite	175:191	arg1	films					193:197	potato starch based bio-nanocomposite films	155:197	potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials	155:251	In this research casting method was used to prepare potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials.
24747380	6	15	from	break	836:840	arg1	elongation					822:831	elongation	822:831	elongation at break	822:840	Tensile strength was increased from 7.33 to 9.82MPa, and elongation at break decreased from 68.0 to 44.0%.
24747380	4	16	theme	Physicochemical	457:471	arg1	properties					473:482	Physicochemical properties	457:482	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films	457:637	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	16	theme	Physicochemical	457:471	arg1	solubility					492:501	solubility	492:501	solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films	492:637	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	9	17	theme	barrier	1149:1155	arg1	properties					1172:1181	the barrier and mechanical properties	1145:1181	properties	1172:1181	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	7	18	dep	580N/m	963:968	arg1	to					960:961	to	960:961	to	960:961	Solubility in water decreased from 35 to 23%, and heat seal strength increased from 375 to 580N/m.
24747380	2	19	dep	sorbitol/glycerol	324:340	arg1	ratio					350:354	weight ratio	343:354	weight ratio of 3 to 1as plasticizer	343:378	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	1	20	used	used	139:142	arg2	method					128:133	casting method	120:133	casting method	120:133	In this research casting method was used to prepare potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials.
24747380	1	21	theme	halloysite	204:213	arg1	nanoclay					215:222	halloysite nanoclay	204:222	halloysite nanoclay as the reinforcing materials	204:251	In this research casting method was used to prepare potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials.
24747380	4	22	theme	absorption	519:528	arg1	capacity					530:537	water absorption capacity	513:537	water absorption capacity (WAC)	513:543	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	22	theme	absorption	519:528	arg1	WAC					540:542	WAC	540:542	WAC	540:542	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	8	23	theme	clay	993:996	arg1	nanoparticles					998:1010	clay nanoparticles	993:1010	clay nanoparticles	993:1010	Also incorporation of clay nanoparticles in the structure of biopolymer decreased permeability of the gaseous molecules.
24747380	7	24	dep	23	913:914	arg1	to					910:911	to	910:911	to	910:911	Solubility in water decreased from 35 to 23%, and heat seal strength increased from 375 to 580N/m.
24747380	4	25	theme	oxygen	579:584	arg1	permeability					586:597	oxygen permeability	579:597	oxygen permeability	579:597	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	2	26	theme	w/w	401:403	arg1	nanoclay					386:393	nanoclay	386:393	nanoclay (0-5% w/w)	386:404	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	2	26	theme	w/w	401:403	arg1	%					399:399	0-5% w/w	396:403	0-5% w/w	396:403	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	9	27	theme	mechanical	1161:1170	arg1	properties					1172:1181	the barrier and mechanical properties	1145:1181	properties	1172:1181	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	1	28	theme	casting	120:126	arg1	method					128:133	casting method	120:133	casting method	120:133	In this research casting method was used to prepare potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials.
24747380	0	29	theme	films	53:57	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of bionanocomposite films based on potato starch/halloysite nanoclay.
24747380	0	29	theme	films	53:57	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of bionanocomposite films based on potato starch/halloysite nanoclay.
24747380	9	30	theme	food	1268:1271	arg1	purposes					1283:1290	food packaging purposes	1268:1290	food packaging purposes	1268:1290	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	7	31	from	Solubility	872:881	arg1	water					886:890	water	886:890	water	886:890	Solubility in water decreased from 35 to 23%, and heat seal strength increased from 375 to 580N/m.
24747380	9	32	contain	have	1233:1236	arg2	potential					1243:1251	high potential	1238:1251	high potential to be used for food packaging purposes	1238:1290	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	9	32	contain	have	1233:1236	arg1	bionanocomposites					1215:1231	this bionanocomposites	1210:1231	this bionanocomposites	1210:1231	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	0	33	theme	bionanocomposite	36:51	arg1	films					53:57	bionanocomposite films	36:57	bionanocomposite films	36:57	Preparation and characterization of bionanocomposite films based on potato starch/halloysite nanoclay.
24747380	6	34	dep	44.0	865:868	arg1	to					862:863	to	862:863	to	862:863	Tensile strength was increased from 7.33 to 9.82MPa, and elongation at break decreased from 68.0 to 44.0%.
24747380	8	35	theme	nanoparticles	998:1010	arg1	incorporation					976:988	incorporation	976:988	incorporation of clay nanoparticles in the structure of biopolymer	976:1041	Also incorporation of clay nanoparticles in the structure of biopolymer decreased permeability of the gaseous molecules.
24747380	7	36	theme	seal	927:930	arg1	strength					932:939	heat seal strength	922:939	heat seal strength	922:939	Solubility in water decreased from 35 to 23%, and heat seal strength increased from 375 to 580N/m.
24747380	1	37	theme	reinforcing	231:241	arg1	materials					243:251	the reinforcing materials	227:251	the reinforcing materials	227:251	In this research casting method was used to prepare potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials.
24747380	5	38	theme	films	744:748	arg1	properties					730:739	mechanical properties	719:739	mechanical properties of films	719:748	Results showed that by increasing the concentration of nanoclay, mechanical properties of films were improved.
24747380	8	39	from	incorporation	976:988	arg1	structure					1019:1027	the structure	1015:1027	the structure of biopolymer	1015:1041	Also incorporation of clay nanoparticles in the structure of biopolymer decreased permeability of the gaseous molecules.
24747380	5	40	theme	nanoclay	709:716	arg1	concentration					692:704	the concentration	688:704	the concentration of nanoclay	688:716	Results showed that by increasing the concentration of nanoclay, mechanical properties of films were improved.
24747380	2	41	theme	weight	343:348	arg1	ratio					350:354	weight ratio	343:354	weight ratio of 3 to 1as plasticizer	343:378	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	4	42	theme	water	513:517	arg1	capacity					530:537	water absorption capacity	513:537	water absorption capacity (WAC)	513:543	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	42	theme	water	513:517	arg1	WAC					540:542	WAC	540:542	WAC	540:542	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	43	theme	vapour	552:557	arg1	WVP					573:575	WVP	573:575	WVP	573:575	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	43	theme	vapour	552:557	arg1	permeability					559:570	water vapour permeability	546:570	water vapour permeability (WVP)	546:576	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	2	44	with	starch	286:291	arg1	w/w					303:305	w/w	303:305	w/w	303:305	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	2	44	with	starch	286:291	arg1	%					300:300	40%	298:300	40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w)	298:404	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	2	44	with	starch	286:291	arg1	mixture					313:319	a mixture	311:319	a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w)	311:404	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	8	45	theme	biopolymer	1032:1041	arg1	structure					1019:1027	the structure	1015:1027	the structure of biopolymer	1015:1041	Also incorporation of clay nanoparticles in the structure of biopolymer decreased permeability of the gaseous molecules.
24747380	9	46	theme	halloysite	1116:1125	arg1	nanoclay					1127:1134	halloysite nanoclay	1116:1134	halloysite nanoclay	1116:1134	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	7	47	theme	heat	922:925	arg1	strength					932:939	heat seal strength	922:939	heat seal strength	922:939	Solubility in water decreased from 35 to 23%, and heat seal strength increased from 375 to 580N/m.
24747380	2	48	theme	sorbitol/glycerol	324:340	arg1	mixture					313:319	a mixture	311:319	a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w)	311:404	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	1	49	with	films	193:197	arg1	nanoclay					215:222	halloysite nanoclay	204:222	halloysite nanoclay as the reinforcing materials	204:251	In this research casting method was used to prepare potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials.
24747380	9	50	theme	potato	1186:1191	arg1	films					1200:1204	potato starch films	1186:1204	potato starch films	1186:1204	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	1	51	theme	potato	155:160	arg1	films					193:197	potato starch based bio-nanocomposite films	155:197	potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials	155:251	In this research casting method was used to prepare potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials.
24747380	3	52	theme	controlled	434:443	arg1	conditions					445:454	controlled conditions	434:454	controlled conditions	434:454	The films were dried under controlled conditions.
24747380	0	53	theme	starch/halloysite	75:91	arg1	nanoclay					93:100	potato starch/halloysite nanoclay	68:100	potato starch/halloysite nanoclay	68:100	Preparation and characterization of bionanocomposite films based on potato starch/halloysite nanoclay.
24747380	9	54	theme	starch	1193:1198	arg1	films					1200:1204	potato starch films	1186:1204	potato starch films	1186:1204	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	2	55	theme	potato	279:284	arg1	starch					286:291	potato starch	279:291	potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w)	279:404	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	4	56	theme	films	633:637	arg1	permeability					586:597	oxygen permeability	579:597	oxygen permeability	579:597	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	56	theme	films	633:637	arg1	capacity					530:537	water absorption capacity	513:537	water absorption capacity (WAC)	513:543	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	56	theme	films	633:637	arg1	permeability					559:570	water vapour permeability	546:570	water vapour permeability (WVP)	546:576	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	56	theme	films	633:637	arg1	WAC					540:542	WAC	540:542	WAC	540:542	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	56	theme	films	633:637	arg1	WVP					573:575	WVP	573:575	WVP	573:575	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	56	theme	films	633:637	arg1	water					506:510	water	506:510	water	506:510	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	4	56	theme	films	633:637	arg1	properties					615:624	mechanical properties	604:624	mechanical properties of the films	604:637	Physicochemical properties such as solubility in water, water absorption capacity (WAC), water vapour permeability (WVP), oxygen permeability, and mechanical properties of the films were measured.
24747380	0	57	theme	potato	68:73	arg1	nanoclay					93:100	potato starch/halloysite nanoclay	68:100	potato starch/halloysite nanoclay	68:100	Preparation and characterization of bionanocomposite films based on potato starch/halloysite nanoclay.
24747380	9	58	theme	films	1200:1204	arg1	properties					1172:1181	the barrier and mechanical properties	1145:1181	properties	1172:1181	In summary, addition of halloysite nanoclay, improve the barrier and mechanical properties of potato starch films and this bionanocomposites have high potential to be used for food packaging purposes.
24747380	2	59	theme	3	359:359	arg1	ratio					350:354	weight ratio	343:354	weight ratio of 3 to 1as plasticizer	343:378	The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w).
24747380	8	60	theme	gaseous	1073:1079	arg1	molecules					1081:1089	the gaseous molecules	1069:1089	the gaseous molecules	1069:1089	Also incorporation of clay nanoparticles in the structure of biopolymer decreased permeability of the gaseous molecules.
26258708	0	0	theme	pigmented	83:91	arg1	rice					93:96	pigmented rice	83:96	pigmented rice	83:96	Polishing and parboiling effect on the nutritional and technological properties of pigmented rice.
26258708	1	1	theme	rice	318:321	arg1	lineage					323:329	MPB-10 red rice lineage	307:329	MPB-10 red rice lineage	307:329	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	7	2	from	decrease	1046:1053	arg1	time					1070:1073	the cooking time	1058:1073	the cooking time of black rice	1058:1087	Parboiling induced an increase in the cooking time of red rice, but a decrease in the cooking time of black rice.
26258708	1	3	theme	polishing	142:150	arg1	effects					131:137	the effects	127:137	the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage	127:329	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	5	4	from	crystallinity	694:706	arg1	black					716:720	black	716:720	black	716:720	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	5	4	from	crystallinity	694:706	arg1	rice					730:733	red rice	726:733	red rice	726:733	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	1	5	theme	lineage	323:329	arg1	compounds					213:221	phenolic compounds	204:221	phenolic compounds	204:221	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	5	theme	lineage	323:329	arg1	composition					180:190	proximate composition	170:190	proximate composition	170:190	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	5	theme	lineage	323:329	arg1	hardness					263:270	hardness	263:270	hardness	263:270	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	5	theme	lineage	323:329	arg1	activity					236:243	antioxidant activity	224:243	antioxidant activity	224:243	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	5	theme	lineage	323:329	arg1	structure					193:201	structure	193:201	structure	193:201	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	5	theme	lineage	323:329	arg1	time					254:257	cooking time	246:257	cooking time	246:257	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	2	6	theme	Proximate	332:340	arg1	analysis					342:349	Proximate analysis	332:349	Proximate analysis	332:349	Proximate analysis and light micrographs revealed higher migration of red rice proteins than black rice proteins to the endosperm as a result of parboiling.
26258708	1	7	theme	cooking	246:252	arg1	time					254:257	cooking time	246:257	cooking time	246:257	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	6	8	theme	polished	961:968	arg1	rice					970:973	polished rice	961:973	polished rice	961:973	Polishing removed more than 90% of free phenolics for both genotypes, while parboiling allowed the partial preservation of free phenolics content in polished rice.
26258708	0	9	theme	rice	93:96	arg1	properties					69:78	the nutritional and technological properties	35:78	the nutritional and technological properties of pigmented rice	35:96	Polishing and parboiling effect on the nutritional and technological properties of pigmented rice.
26258708	7	10	theme	cooking	1062:1068	arg1	time					1070:1073	the cooking time	1058:1073	the cooking time of black rice	1058:1087	Parboiling induced an increase in the cooking time of red rice, but a decrease in the cooking time of black rice.
26258708	1	11	from	effects	131:137	arg1	compounds					213:221	phenolic compounds	204:221	phenolic compounds	204:221	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	11	from	effects	131:137	arg1	composition					180:190	proximate composition	170:190	proximate composition	170:190	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	11	from	effects	131:137	arg1	hardness					263:270	hardness	263:270	hardness	263:270	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	11	from	effects	131:137	arg1	activity					236:243	antioxidant activity	224:243	antioxidant activity	224:243	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	11	from	effects	131:137	arg1	structure					193:201	structure	193:201	structure	193:201	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	11	from	effects	131:137	arg1	time					254:257	cooking time	246:257	cooking time	246:257	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	3	12	theme	red	527:529	arg1	rice					531:534	red rice	527:534	red rice	527:534	Parboiling reduced the ash content of red rice while no difference was determined in black rice.
26258708	3	13	theme	ash	512:514	arg1	content					516:522	the ash content	508:522	the ash content of red rice	508:534	Parboiling reduced the ash content of red rice while no difference was determined in black rice.
26258708	1	14	theme	parboiling	156:165	arg1	effects					131:137	the effects	127:137	the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage	127:329	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	5	15	theme	red	726:728	arg1	rice					730:733	red rice	726:733	red rice	726:733	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	6	16	theme	phenolics	852:860	arg1	%					842:842	more than 90%	830:842	more than 90% of free phenolics	830:860	Polishing removed more than 90% of free phenolics for both genotypes, while parboiling allowed the partial preservation of free phenolics content in polished rice.
26258708	6	16	theme	phenolics	852:860	arg1	phenolics					852:860	free phenolics	847:860	free phenolics	847:860	Polishing removed more than 90% of free phenolics for both genotypes, while parboiling allowed the partial preservation of free phenolics content in polished rice.
26258708	2	17	theme	higher	382:387	arg1	migration					389:397	higher migration	382:397	higher migration of red rice proteins	382:418	Proximate analysis and light micrographs revealed higher migration of red rice proteins than black rice proteins to the endosperm as a result of parboiling.
26258708	5	18	theme	indicative	773:782	arg1	decrease					673:680	a decrease	671:680	a decrease	671:680	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	5	18	theme	indicative	773:782	arg1	disruption					800:809	an indicative of crystallites disruption	770:809	an indicative of crystallites disruption	770:809	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	6	19	theme	free	847:850	arg1	phenolics					852:860	free phenolics	847:860	free phenolics	847:860	Polishing removed more than 90% of free phenolics for both genotypes, while parboiling allowed the partial preservation of free phenolics content in polished rice.
26258708	1	20	theme	proximate	170:178	arg1	composition					180:190	proximate composition	170:190	proximate composition	170:190	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	2	21	theme	rice	431:434	arg1	proteins					436:443	black rice proteins	425:443	black rice proteins	425:443	Proximate analysis and light micrographs revealed higher migration of red rice proteins than black rice proteins to the endosperm as a result of parboiling.
26258708	6	22	theme	content	950:956	arg1	preservation					919:930	the partial preservation	907:930	the partial preservation of free phenolics content in polished rice	907:973	Polishing removed more than 90% of free phenolics for both genotypes, while parboiling allowed the partial preservation of free phenolics content in polished rice.
26258708	6	23	from	rice	970:973	arg1	preservation					919:930	the partial preservation	907:930	the partial preservation of free phenolics content in polished rice	907:973	Polishing removed more than 90% of free phenolics for both genotypes, while parboiling allowed the partial preservation of free phenolics content in polished rice.
26258708	4	24	from	genotypes	624:632	arg1	granules					605:612	Gelatinized starch granules	586:612	Gelatinized starch granules from both genotypes	586:632	Gelatinized starch granules from both genotypes showed similar appearance.
26258708	2	25	theme	black	425:429	arg1	proteins					436:443	black rice proteins	425:443	black rice proteins	425:443	Proximate analysis and light micrographs revealed higher migration of red rice proteins than black rice proteins to the endosperm as a result of parboiling.
26258708	6	26	theme	phenolics	940:948	arg1	content					950:956	free phenolics content	935:956	free phenolics content in polished rice	935:973	Polishing removed more than 90% of free phenolics for both genotypes, while parboiling allowed the partial preservation of free phenolics content in polished rice.
26258708	7	27	theme	rice	1034:1037	arg1	time					1022:1025	the cooking time	1010:1025	the cooking time of red rice	1010:1037	Parboiling induced an increase in the cooking time of red rice, but a decrease in the cooking time of black rice.
26258708	3	28	theme	rice	531:534	arg1	content					516:522	the ash content	508:522	the ash content of red rice	508:534	Parboiling reduced the ash content of red rice while no difference was determined in black rice.
26258708	2	29	theme	proteins	411:418	arg1	migration					389:397	higher migration	382:397	higher migration of red rice proteins	382:418	Proximate analysis and light micrographs revealed higher migration of red rice proteins than black rice proteins to the endosperm as a result of parboiling.
26258708	6	30	theme	free	935:938	arg1	content					950:956	free phenolics content	935:956	free phenolics content in polished rice	935:973	Polishing removed more than 90% of free phenolics for both genotypes, while parboiling allowed the partial preservation of free phenolics content in polished rice.
26258708	5	31	theme	relative	685:692	arg1	crystallinity					694:706	relative crystallinity	685:706	relative crystallinity on both black and red rice subjected to parboiling	685:757	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	5	32	theme	crystallites	787:798	arg1	indicative					773:782	indicative	773:782	indicative	773:782	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	2	33	theme	rice	406:409	arg1	proteins					411:418	red rice proteins	402:418	red rice proteins	402:418	Proximate analysis and light micrographs revealed higher migration of red rice proteins than black rice proteins to the endosperm as a result of parboiling.
26258708	1	34	theme	black	283:287	arg1	cultivar					294:301	IAC-600 black rice cultivar	275:301	IAC-600 black rice cultivar	275:301	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	6	35	from	content	950:956	arg1	rice					970:973	polished rice	961:973	polished rice	961:973	Polishing removed more than 90% of free phenolics for both genotypes, while parboiling allowed the partial preservation of free phenolics content in polished rice.
26258708	2	36	theme	red	402:404	arg1	proteins					411:418	red rice proteins	402:418	red rice proteins	402:418	Proximate analysis and light micrographs revealed higher migration of red rice proteins than black rice proteins to the endosperm as a result of parboiling.
26258708	4	37	theme	similar	641:647	arg1	appearance					649:658	similar appearance	641:658	similar appearance	641:658	Gelatinized starch granules from both genotypes showed similar appearance.
26258708	1	38	theme	rice	289:292	arg1	cultivar					294:301	IAC-600 black rice cultivar	275:301	IAC-600 black rice cultivar	275:301	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	7	39	theme	red	1030:1032	arg1	rice					1034:1037	red rice	1030:1037	red rice	1030:1037	Parboiling induced an increase in the cooking time of red rice, but a decrease in the cooking time of black rice.
26258708	3	40	theme	black	574:578	arg1	rice					580:583	black rice	574:583	black rice	574:583	Parboiling reduced the ash content of red rice while no difference was determined in black rice.
26258708	1	41	theme	phenolic	204:211	arg1	compounds					213:221	phenolic compounds	204:221	phenolic compounds	204:221	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	7	42	theme	black	1078:1082	arg1	rice					1084:1087	black rice	1078:1087	black rice	1078:1087	Parboiling induced an increase in the cooking time of red rice, but a decrease in the cooking time of black rice.
26258708	1	43	theme	cultivar	294:301	arg1	compounds					213:221	phenolic compounds	204:221	phenolic compounds	204:221	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	43	theme	cultivar	294:301	arg1	composition					180:190	proximate composition	170:190	proximate composition	170:190	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	43	theme	cultivar	294:301	arg1	hardness					263:270	hardness	263:270	hardness	263:270	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	43	theme	cultivar	294:301	arg1	activity					236:243	antioxidant activity	224:243	antioxidant activity	224:243	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	43	theme	cultivar	294:301	arg1	structure					193:201	structure	193:201	structure	193:201	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	1	43	theme	cultivar	294:301	arg1	time					254:257	cooking time	246:257	cooking time	246:257	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	7	44	theme	cooking	1014:1020	arg1	time					1022:1025	the cooking time	1010:1025	the cooking time of red rice	1010:1037	Parboiling induced an increase in the cooking time of red rice, but a decrease in the cooking time of black rice.
26258708	5	45	from	black	716:720	arg1	decrease					673:680	a decrease	671:680	a decrease	671:680	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	5	45	from	black	716:720	arg1	disruption					800:809	an indicative of crystallites disruption	770:809	an indicative of crystallites disruption	770:809	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	0	46	theme	nutritional	39:49	arg1	properties					69:78	the nutritional and technological properties	35:78	the nutritional and technological properties of pigmented rice	35:96	Polishing and parboiling effect on the nutritional and technological properties of pigmented rice.
26258708	1	47	theme	red	314:316	arg1	lineage					323:329	MPB-10 red rice lineage	307:329	MPB-10 red rice lineage	307:329	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	6	48	from	preservation	919:930	arg1	rice					970:973	polished rice	961:973	polished rice	961:973	Polishing removed more than 90% of free phenolics for both genotypes, while parboiling allowed the partial preservation of free phenolics content in polished rice.
26258708	7	49	theme	rice	1084:1087	arg1	time					1070:1073	the cooking time	1058:1073	the cooking time of black rice	1058:1087	Parboiling induced an increase in the cooking time of red rice, but a decrease in the cooking time of black rice.
26258708	5	50	from	rice	730:733	arg1	decrease					673:680	a decrease	671:680	a decrease	671:680	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	5	50	from	rice	730:733	arg1	disruption					800:809	an indicative of crystallites disruption	770:809	an indicative of crystallites disruption	770:809	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	5	51	from	decrease	673:680	arg1	crystallinity					694:706	relative crystallinity	685:706	relative crystallinity on both black and red rice subjected to parboiling	685:757	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	5	51	from	decrease	673:680	arg1	black					716:720	black	716:720	black	716:720	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	5	51	from	decrease	673:680	arg1	rice					730:733	red rice	726:733	red rice	726:733	There was a decrease in relative crystallinity on both black and red rice subjected to parboiling, which was an indicative of crystallites disruption.
26258708	2	52	theme	parboiling	477:486	arg1	result					467:472	a result	465:472	a result of parboiling	465:486	Proximate analysis and light micrographs revealed higher migration of red rice proteins than black rice proteins to the endosperm as a result of parboiling.
26258708	7	53	from	increase	998:1005	arg1	time					1022:1025	the cooking time	1010:1025	the cooking time of red rice	1010:1037	Parboiling induced an increase in the cooking time of red rice, but a decrease in the cooking time of black rice.
26258708	4	54	theme	starch	598:603	arg1	granules					605:612	Gelatinized starch granules	586:612	Gelatinized starch granules from both genotypes	586:632	Gelatinized starch granules from both genotypes showed similar appearance.
26258708	2	55	theme	light	355:359	arg1	micrographs					361:371	light micrographs	355:371	light micrographs	355:371	Proximate analysis and light micrographs revealed higher migration of red rice proteins than black rice proteins to the endosperm as a result of parboiling.
26258708	1	56	theme	antioxidant	224:234	arg1	activity					236:243	antioxidant activity	224:243	antioxidant activity	224:243	This study aims to evaluate the effects of polishing and parboiling on proximate composition, structure, phenolic compounds, antioxidant activity, cooking time and hardness of IAC-600 black rice cultivar and MPB-10 red rice lineage.
26258708	0	57	theme	technological	55:67	arg1	properties					69:78	the nutritional and technological properties	35:78	the nutritional and technological properties of pigmented rice	35:96	Polishing and parboiling effect on the nutritional and technological properties of pigmented rice.
26258708	0	58	from	effect	25:30	arg1	properties					69:78	the nutritional and technological properties	35:78	the nutritional and technological properties of pigmented rice	35:96	Polishing and parboiling effect on the nutritional and technological properties of pigmented rice.
26258708	6	59	theme	partial	911:917	arg1	preservation					919:930	the partial preservation	907:930	the partial preservation of free phenolics content in polished rice	907:973	Polishing removed more than 90% of free phenolics for both genotypes, while parboiling allowed the partial preservation of free phenolics content in polished rice.
26258708	4	60	theme	Gelatinized	586:596	arg1	granules					605:612	Gelatinized starch granules	586:612	Gelatinized starch granules from both genotypes	586:632	Gelatinized starch granules from both genotypes showed similar appearance.
27455406	9	0	theme	down	1665:1668	arg1	chains					1670:1675	lain down chains	1660:1675	lain down chains	1660:1675	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	2	1	theme	coatings	384:391	arg1	nanostructure					351:363	the nanostructure	347:363	the nanostructure of these ultrathin coatings	347:391	Nevertheless, the nanostructure of these ultrathin coatings and its tailoring by the variation of the preparation conditions have not been profoundly characterized and understood.
27455406	2	1	theme	coatings	384:391	arg1	tailoring					401:409	its tailoring	397:409	its tailoring by the variation of the preparation conditions	397:456	Nevertheless, the nanostructure of these ultrathin coatings and its tailoring by the variation of the preparation conditions have not been profoundly characterized and understood.
27455406	1	2	theme	binding	292:298	arg1	suppression					264:274	the suppression	260:274	the suppression of non-specific binding	260:298	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	10	3	theme	surface	2025:2031	arg1	layers					2033:2038	highly hydrated surface layers	2009:2038	highly hydrated surface layers	2009:2038	The developed methodologies allowed to design and fabricate nanometer scale CMD layers with well-controlled surface structure, which are very difficult to characterize in aqueous environments using present instrumentations and highly hydrated surface layers.
27455406	5	4	theme	additional	927:936	arg1	reagents					938:945	any additional reagents	923:945	any additional reagents	923:945	The carboxyl moiety of the CMD was coupled to the aminated surface by EDC-NHS reagents, while CMD coupling through epoxysilane molecules was performed without any additional reagents.
27455406	4	5	theme	aminosilane-	672:683	arg1	surfaces					716:723	aminosilane- and epoxysilane-functionalized surfaces	672:723	aminosilane- and epoxysilane-functionalized surfaces	672:723	A grafting method based on covalent coupling to aminosilane- and epoxysilane-functionalized surfaces was applied to obtain thin CMD layers.
27455406	3	6	theme	ultrathin	596:604	arg1	coatings					614:621	fabricating ultrathin surface coatings	584:621	fabricating ultrathin surface coatings	584:621	In this work carboxymethylated dextran (CMD) was prepared and used for fabricating ultrathin surface coatings.
27455406	5	7	theme	epoxysilane	879:889	arg1	molecules					891:899	epoxysilane molecules	879:899	epoxysilane molecules	879:899	The carboxyl moiety of the CMD was coupled to the aminated surface by EDC-NHS reagents, while CMD coupling through epoxysilane molecules was performed without any additional reagents.
27455406	6	8	theme	photoelectron	1022:1034	arg1	spectroscopy					1036:1047	X-ray photoelectron spectroscopy	1016:1047	X-ray photoelectron spectroscopy (XPS)	1016:1053	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	6	8	theme	photoelectron	1022:1034	arg1	XPS					1050:1052	XPS	1050:1052	XPS	1050:1052	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	0	9	from	nanostructure	77:89	arg1	environments					102:113	aqueous environments	94:113	aqueous environments revealed by OWLS	94:130	Fabrication and characterization of ultrathin dextran layers: Time dependent nanostructure in aqueous environments revealed by OWLS.
27455406	4	10	theme	epoxysilane-functionalized	689:714	arg1	surfaces					716:723	aminosilane- and epoxysilane-functionalized surfaces	672:723	aminosilane- and epoxysilane-functionalized surfaces	672:723	A grafting method based on covalent coupling to aminosilane- and epoxysilane-functionalized surfaces was applied to obtain thin CMD layers.
27455406	9	11	theme	chemical	1536:1543	arg1	composition					1545:1555	the chemical composition	1532:1555	the chemical composition of the silane coverage	1532:1578	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	8	12	theme	OWLS	1344:1347	arg1	method					1349:1354	The OWLS method	1340:1354	The OWLS method	1340:1354	The OWLS method allowed devising the structure of the interfacial dextran layers by the evaluation of the optogeometrical parameters.
27455406	9	13	theme	epoxysilylated	1757:1770	arg1	surfaces					1772:1779	epoxysilylated surfaces	1757:1779	epoxysilylated surfaces	1757:1779	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	4	14	theme	thin	747:750	arg1	layers					756:761	thin CMD layers	747:761	thin CMD layers	747:761	A grafting method based on covalent coupling to aminosilane- and epoxysilane-functionalized surfaces was applied to obtain thin CMD layers.
27455406	10	15	theme	scale	1852:1856	arg1	layers					1862:1867	nanometer scale CMD layers	1842:1867	nanometer scale CMD layers	1842:1867	The developed methodologies allowed to design and fabricate nanometer scale CMD layers with well-controlled surface structure, which are very difficult to characterize in aqueous environments using present instrumentations and highly hydrated surface layers.
27455406	9	16	theme	silane	1564:1569	arg1	coverage					1571:1578	the silane coverage	1560:1578	the silane coverage	1560:1578	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	6	17	theme	reflection	1073:1082	arg1	ATR-IR					1107:1112	ATR-IR	1107:1112	ATR-IR	1107:1112	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	6	17	theme	reflection	1073:1082	arg1	spectroscopy					1093:1104	attenuated total reflection infrared spectroscopy	1056:1104	attenuated total reflection infrared spectroscopy (ATR-IR)	1056:1113	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	5	18	theme	aminated	814:821	arg1	surface					823:829	the aminated surface	810:829	the aminated surface	810:829	The carboxyl moiety of the CMD was coupled to the aminated surface by EDC-NHS reagents, while CMD coupling through epoxysilane molecules was performed without any additional reagents.
27455406	1	19	theme	dextran	172:178	arg1	ingredients					208:218	key ingredients	204:218	key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization	204:330	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	1	19	theme	dextran	172:178	arg1	coatings					141:148	Surface coatings	133:148	Surface coatings of the polysaccharide dextran and its derivatives	133:198	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	6	20	theme	attenuated	1056:1065	arg1	ATR-IR					1107:1112	ATR-IR	1107:1112	ATR-IR	1107:1112	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	6	20	theme	attenuated	1056:1065	arg1	spectroscopy					1093:1104	attenuated total reflection infrared spectroscopy	1056:1104	attenuated total reflection infrared spectroscopy (ATR-IR)	1056:1113	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	0	21	theme	aqueous	94:100	arg1	environments					102:113	aqueous environments	94:113	aqueous environments revealed by OWLS	94:130	Fabrication and characterization of ultrathin dextran layers: Time dependent nanostructure in aqueous environments revealed by OWLS.
27455406	8	22	theme	interfacial	1394:1404	arg1	layers					1414:1419	the interfacial dextran layers	1390:1419	the interfacial dextran layers	1390:1419	The OWLS method allowed devising the structure of the interfacial dextran layers by the evaluation of the optogeometrical parameters.
27455406	6	23	theme	atomic	1144:1149	arg1	AFM					1169:1171	AFM	1169:1171	AFM	1169:1171	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	6	23	theme	atomic	1144:1149	arg1	microscopy					1157:1166	atomic force microscopy	1144:1166	atomic force microscopy (AFM)	1144:1172	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	8	24	theme	layers	1414:1419	arg1	structure					1377:1385	the structure	1373:1385	the structure of the interfacial dextran layers	1373:1419	The OWLS method allowed devising the structure of the interfacial dextran layers by the evaluation of the optogeometrical parameters.
27455406	10	25	theme	present	1980:1986	arg1	instrumentations					1988:2003	present instrumentations	1980:2003	present instrumentations	1980:2003	The developed methodologies allowed to design and fabricate nanometer scale CMD layers with well-controlled surface structure, which are very difficult to characterize in aqueous environments using present instrumentations and highly hydrated surface layers.
27455406	9	26	theme	solution	1607:1614	arg1	pH					1588:1589	the pH	1584:1589	the pH of the grafting solution	1584:1614	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	9	26	theme	solution	1607:1614	arg1	alteration					1478:1487	The alteration	1474:1487	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage	1474:1578	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	1	27	theme	receptor	308:315	arg1	immobilization					317:330	receptor immobilization	308:330	receptor immobilization	308:330	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	9	28	from	alteration	1478:1487	arg1	nanostructure					1496:1508	the nanostructure	1492:1508	the nanostructure of the CMD layer with the chemical composition of the silane coverage	1492:1578	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	2	29	theme	preparation	435:445	arg1	conditions					447:456	the preparation conditions	431:456	the preparation conditions	431:456	Nevertheless, the nanostructure of these ultrathin coatings and its tailoring by the variation of the preparation conditions have not been profoundly characterized and understood.
27455406	0	30	theme	ultrathin	36:44	arg1	layers					54:59	ultrathin dextran layers	36:59	ultrathin dextran layers	36:59	Fabrication and characterization of ultrathin dextran layers: Time dependent nanostructure in aqueous environments revealed by OWLS.
27455406	9	31	theme	in	1632:1633	arg1	OWLS					1640:1643	in situ OWLS	1632:1643	in situ OWLS	1632:1643	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	9	32	with	nanostructure	1496:1508	arg1	composition					1545:1555	the chemical composition	1532:1555	the chemical composition of the silane coverage	1532:1578	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	10	33	theme	well-controlled	1874:1888	arg1	structure					1898:1906	well-controlled surface structure	1874:1906	well-controlled surface structure	1874:1906	The developed methodologies allowed to design and fabricate nanometer scale CMD layers with well-controlled surface structure, which are very difficult to characterize in aqueous environments using present instrumentations and highly hydrated surface layers.
27455406	8	34	theme	optogeometrical	1446:1460	arg1	parameters					1462:1471	the optogeometrical parameters	1442:1471	the optogeometrical parameters	1442:1471	The OWLS method allowed devising the structure of the interfacial dextran layers by the evaluation of the optogeometrical parameters.
27455406	3	35	theme	carboxymethylated	526:542	arg1	CMD					553:555	CMD	553:555	CMD	553:555	In this work carboxymethylated dextran (CMD) was prepared and used for fabricating ultrathin surface coatings.
27455406	3	35	theme	carboxymethylated	526:542	arg1	dextran					544:550	carboxymethylated dextran	526:550	carboxymethylated dextran (CMD)	526:556	In this work carboxymethylated dextran (CMD) was prepared and used for fabricating ultrathin surface coatings.
27455406	0	36	theme	layers	54:59	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of ultrathin dextran layers: Time dependent nanostructure in aqueous environments revealed by OWLS.
27455406	0	36	theme	layers	54:59	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of ultrathin dextran layers: Time dependent nanostructure in aqueous environments revealed by OWLS.
27455406	6	37	theme	waveguide	1186:1194	arg1	OWLS					1220:1223	OWLS	1220:1223	OWLS	1220:1223	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	6	37	theme	waveguide	1186:1194	arg1	spectroscopy					1206:1217	optical waveguide lightmode spectroscopy	1178:1217	optical waveguide lightmode spectroscopy (OWLS)	1178:1224	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	1	38	theme	label-free	234:243	arg1	biosensors					245:254	label-free biosensors	234:254	label-free biosensors	234:254	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	6	39	theme	grafting	983:990	arg1	procedures					992:1001	the grafting procedures	979:1001	the grafting procedures	979:1001	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	9	40	theme	lain	1660:1663	arg1	chains					1670:1675	lain down chains	1660:1675	lain down chains	1660:1675	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	7	41	theme	dextran	1305:1311	arg1	layer					1313:1317	a very thin dextran layer	1293:1317	a very thin dextran layer of a few nanometers	1293:1337	The XPS and AFM measurements showed that the grafting resulted in a very thin dextran layer of a few nanometers.
27455406	4	42	theme	covalent	651:658	arg1	coupling					660:667	covalent coupling	651:667	covalent coupling to aminosilane- and epoxysilane-functionalized surfaces	651:723	A grafting method based on covalent coupling to aminosilane- and epoxysilane-functionalized surfaces was applied to obtain thin CMD layers.
27455406	1	43	theme	non-specific	279:290	arg1	binding					292:298	non-specific binding	279:298	non-specific binding	279:298	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	6	44	theme	surface	952:958	arg1	analysis					960:967	The surface analysis	948:967	The surface analysis following the grafting procedures	948:1001	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	9	45	theme	coverage	1571:1578	arg1	composition					1545:1555	the chemical composition	1532:1555	the chemical composition of the silane coverage	1532:1578	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	4	46	theme	grafting	626:633	arg1	method					635:640	A grafting method	624:640	A grafting method based on covalent coupling to aminosilane- and epoxysilane-functionalized surfaces	624:723	A grafting method based on covalent coupling to aminosilane- and epoxysilane-functionalized surfaces was applied to obtain thin CMD layers.
27455406	2	47	theme	ultrathin	374:382	arg1	coatings					384:391	these ultrathin coatings	368:391	these ultrathin coatings	368:391	Nevertheless, the nanostructure of these ultrathin coatings and its tailoring by the variation of the preparation conditions have not been profoundly characterized and understood.
27455406	3	48	theme	surface	606:612	arg1	coatings					614:621	fabricating ultrathin surface coatings	584:621	fabricating ultrathin surface coatings	584:621	In this work carboxymethylated dextran (CMD) was prepared and used for fabricating ultrathin surface coatings.
27455406	1	49	theme	derivatives	188:198	arg1	ingredients					208:218	key ingredients	204:218	key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization	204:330	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	1	49	theme	derivatives	188:198	arg1	coatings					141:148	Surface coatings	133:148	Surface coatings of the polysaccharide dextran and its derivatives	133:198	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	3	50	used	used	575:578	arg2	CMD					553:555	CMD	553:555	CMD	553:555	In this work carboxymethylated dextran (CMD) was prepared and used for fabricating ultrathin surface coatings.
27455406	3	50	used	used	575:578	arg2	dextran					544:550	carboxymethylated dextran	526:550	carboxymethylated dextran (CMD)	526:556	In this work carboxymethylated dextran (CMD) was prepared and used for fabricating ultrathin surface coatings.
27455406	10	51	theme	hydrated	2016:2023	arg1	layers					2033:2038	highly hydrated surface layers	2009:2038	highly hydrated surface layers	2009:2038	The developed methodologies allowed to design and fabricate nanometer scale CMD layers with well-controlled surface structure, which are very difficult to characterize in aqueous environments using present instrumentations and highly hydrated surface layers.
27455406	3	52	theme	fabricating	584:594	arg1	coatings					614:621	fabricating ultrathin surface coatings	584:621	fabricating ultrathin surface coatings	584:621	In this work carboxymethylated dextran (CMD) was prepared and used for fabricating ultrathin surface coatings.
27455406	1	53	theme	key	204:206	arg1	ingredients					208:218	key ingredients	204:218	key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization	204:330	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	1	53	theme	key	204:206	arg1	coatings					141:148	Surface coatings	133:148	Surface coatings of the polysaccharide dextran and its derivatives	133:198	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	7	54	theme	few	1324:1326	arg1	nanometers					1328:1337	a few nanometers	1322:1337	a few nanometers	1322:1337	The XPS and AFM measurements showed that the grafting resulted in a very thin dextran layer of a few nanometers.
27455406	5	55	theme	carboxyl	768:775	arg1	moiety					777:782	The carboxyl moiety	764:782	The carboxyl moiety of the CMD	764:793	The carboxyl moiety of the CMD was coupled to the aminated surface by EDC-NHS reagents, while CMD coupling through epoxysilane molecules was performed without any additional reagents.
27455406	10	56	theme	nanometer	1842:1850	arg1	layers					1862:1867	nanometer scale CMD layers	1842:1867	nanometer scale CMD layers	1842:1867	The developed methodologies allowed to design and fabricate nanometer scale CMD layers with well-controlled surface structure, which are very difficult to characterize in aqueous environments using present instrumentations and highly hydrated surface layers.
27455406	9	57	dep	in	1632:1633	arg1	situ					1635:1638	situ	1635:1638	situ	1635:1638	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	9	58	theme	layer	1521:1525	arg1	nanostructure					1496:1508	the nanostructure	1492:1508	the nanostructure of the CMD layer with the chemical composition of the silane coverage	1492:1578	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	6	59	theme	X-ray	1016:1020	arg1	spectroscopy					1036:1047	X-ray photoelectron spectroscopy	1016:1047	X-ray photoelectron spectroscopy (XPS)	1016:1053	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	6	59	theme	X-ray	1016:1020	arg1	XPS					1050:1052	XPS	1050:1052	XPS	1050:1052	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	9	60	theme	neutral	1725:1731	arg1	conditions					1743:1752	neutral and basic conditions	1725:1752	neutral and basic conditions	1725:1752	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	9	61	theme	basic	1737:1741	arg1	conditions					1743:1752	neutral and basic conditions	1725:1752	neutral and basic conditions	1725:1752	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	10	62	theme	developed	1786:1794	arg1	methodologies					1796:1808	The developed methodologies	1782:1808	The developed methodologies	1782:1808	The developed methodologies allowed to design and fabricate nanometer scale CMD layers with well-controlled surface structure, which are very difficult to characterize in aqueous environments using present instrumentations and highly hydrated surface layers.
27455406	1	63	from	ingredients	208:218	arg1	biosensors					245:254	label-free biosensors	234:254	label-free biosensors	234:254	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	1	64	theme	Surface	133:139	arg1	ingredients					208:218	key ingredients	204:218	key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization	204:330	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	1	64	theme	Surface	133:139	arg1	coatings					141:148	Surface coatings	133:148	Surface coatings of the polysaccharide dextran and its derivatives	133:198	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	0	65	theme	dependent	67:75	arg1	nanostructure					77:89	Time dependent nanostructure	62:89	Fabrication and characterization of ultrathin dextran layers: Time dependent nanostructure in aqueous environments revealed by OWLS.	0:131	Fabrication and characterization of ultrathin dextran layers: Time dependent nanostructure in aqueous environments revealed by OWLS.
27455406	10	66	theme	CMD	1858:1860	arg1	layers					1862:1867	nanometer scale CMD layers	1842:1867	nanometer scale CMD layers	1842:1867	The developed methodologies allowed to design and fabricate nanometer scale CMD layers with well-controlled surface structure, which are very difficult to characterize in aqueous environments using present instrumentations and highly hydrated surface layers.
27455406	6	67	theme	infrared	1084:1091	arg1	ATR-IR					1107:1112	ATR-IR	1107:1112	ATR-IR	1107:1112	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	6	67	theme	infrared	1084:1091	arg1	spectroscopy					1093:1104	attenuated total reflection infrared spectroscopy	1056:1104	attenuated total reflection infrared spectroscopy (ATR-IR)	1056:1113	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	1	68	theme	polysaccharide	157:170	arg1	dextran					172:178	the polysaccharide dextran	153:178	the polysaccharide dextran	153:178	Surface coatings of the polysaccharide dextran and its derivatives are key ingredients especially in label-free biosensors for the suppression of non-specific binding and for receptor immobilization.
27455406	6	69	theme	total	1067:1071	arg1	ATR-IR					1107:1112	ATR-IR	1107:1112	ATR-IR	1107:1112	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	6	69	theme	total	1067:1071	arg1	spectroscopy					1093:1104	attenuated total reflection infrared spectroscopy	1056:1104	attenuated total reflection infrared spectroscopy (ATR-IR)	1056:1113	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	4	70	theme	CMD	752:754	arg1	layers					756:761	thin CMD layers	747:761	thin CMD layers	747:761	A grafting method based on covalent coupling to aminosilane- and epoxysilane-functionalized surfaces was applied to obtain thin CMD layers.
27455406	9	71	from	pH	1588:1589	arg1	nanostructure					1496:1508	the nanostructure	1492:1508	the nanostructure of the CMD layer with the chemical composition of the silane coverage	1492:1578	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	5	72	theme	CMD	791:793	arg1	moiety					777:782	The carboxyl moiety	764:782	The carboxyl moiety of the CMD	764:793	The carboxyl moiety of the CMD was coupled to the aminated surface by EDC-NHS reagents, while CMD coupling through epoxysilane molecules was performed without any additional reagents.
27455406	9	73	theme	CMD	1517:1519	arg1	layer					1521:1525	the CMD layer	1513:1525	the CMD layer	1513:1525	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	6	74	theme	force	1151:1155	arg1	AFM					1169:1171	AFM	1169:1171	AFM	1169:1171	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	6	74	theme	force	1151:1155	arg1	microscopy					1157:1166	atomic force microscopy	1144:1166	atomic force microscopy (AFM)	1144:1172	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	7	75	theme	AFM	1239:1241	arg1	measurements					1243:1254	The XPS and AFM measurements	1227:1254	The XPS and AFM measurements	1227:1254	The XPS and AFM measurements showed that the grafting resulted in a very thin dextran layer of a few nanometers.
27455406	7	76	theme	XPS	1231:1233	arg1	measurements					1243:1254	The XPS and AFM measurements	1227:1254	The XPS and AFM measurements	1227:1254	The XPS and AFM measurements showed that the grafting resulted in a very thin dextran layer of a few nanometers.
27455406	9	77	theme	grafting	1598:1605	arg1	solution					1607:1614	the grafting solution	1594:1614	the grafting solution	1594:1614	The alteration in the nanostructure of the CMD layer with the chemical composition of the silane coverage and the pH of the grafting solution was revealed by in situ OWLS, specifically, lain down chains were found to be prevalent on the surface under neutral and basic conditions on epoxysilylated surfaces.
27455406	8	78	theme	dextran	1406:1412	arg1	layers					1414:1419	the interfacial dextran layers	1390:1419	the interfacial dextran layers	1390:1419	The OWLS method allowed devising the structure of the interfacial dextran layers by the evaluation of the optogeometrical parameters.
27455406	5	79	theme	CMD	858:860	arg1	coupling					862:869	CMD coupling	858:869	CMD coupling through epoxysilane molecules	858:899	The carboxyl moiety of the CMD was coupled to the aminated surface by EDC-NHS reagents, while CMD coupling through epoxysilane molecules was performed without any additional reagents.
27455406	6	80	theme	spectroscopic	1116:1128	arg1	ellipsometry					1130:1141	spectroscopic ellipsometry	1116:1141	spectroscopic ellipsometry	1116:1141	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	2	81	theme	conditions	447:456	arg1	variation					418:426	the variation	414:426	the variation of the preparation conditions	414:456	Nevertheless, the nanostructure of these ultrathin coatings and its tailoring by the variation of the preparation conditions have not been profoundly characterized and understood.
27455406	0	82	theme	dextran	46:52	arg1	layers					54:59	ultrathin dextran layers	36:59	ultrathin dextran layers	36:59	Fabrication and characterization of ultrathin dextran layers: Time dependent nanostructure in aqueous environments revealed by OWLS.
27455406	7	83	theme	nanometers	1328:1337	arg1	layer					1313:1317	a very thin dextran layer	1293:1317	a very thin dextran layer of a few nanometers	1293:1337	The XPS and AFM measurements showed that the grafting resulted in a very thin dextran layer of a few nanometers.
27455406	10	84	theme	surface	1890:1896	arg1	structure					1898:1906	well-controlled surface structure	1874:1906	well-controlled surface structure	1874:1906	The developed methodologies allowed to design and fabricate nanometer scale CMD layers with well-controlled surface structure, which are very difficult to characterize in aqueous environments using present instrumentations and highly hydrated surface layers.
27455406	5	85	theme	EDC-NHS	834:840	arg1	reagents					842:849	EDC-NHS reagents	834:849	EDC-NHS reagents	834:849	The carboxyl moiety of the CMD was coupled to the aminated surface by EDC-NHS reagents, while CMD coupling through epoxysilane molecules was performed without any additional reagents.
27455406	6	86	theme	lightmode	1196:1204	arg1	OWLS					1220:1223	OWLS	1220:1223	OWLS	1220:1223	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	6	86	theme	lightmode	1196:1204	arg1	spectroscopy					1206:1217	optical waveguide lightmode spectroscopy	1178:1217	optical waveguide lightmode spectroscopy (OWLS)	1178:1224	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	0	87	dep	Fabrication	0:10	arg1	nanostructure					77:89	Time dependent nanostructure	62:89	Fabrication and characterization of ultrathin dextran layers: Time dependent nanostructure in aqueous environments revealed by OWLS.	0:131	Fabrication and characterization of ultrathin dextran layers: Time dependent nanostructure in aqueous environments revealed by OWLS.
27455406	6	88	theme	optical	1178:1184	arg1	OWLS					1220:1223	OWLS	1220:1223	OWLS	1220:1223	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	6	88	theme	optical	1178:1184	arg1	spectroscopy					1206:1217	optical waveguide lightmode spectroscopy	1178:1217	optical waveguide lightmode spectroscopy (OWLS)	1178:1224	The surface analysis following the grafting procedures consisted of X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared spectroscopy (ATR-IR), spectroscopic ellipsometry, atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS).
27455406	8	89	theme	parameters	1462:1471	arg1	evaluation					1428:1437	the evaluation	1424:1437	the evaluation of the optogeometrical parameters	1424:1471	The OWLS method allowed devising the structure of the interfacial dextran layers by the evaluation of the optogeometrical parameters.
27455406	7	90	theme	thin	1300:1303	arg1	layer					1313:1317	a very thin dextran layer	1293:1317	a very thin dextran layer of a few nanometers	1293:1337	The XPS and AFM measurements showed that the grafting resulted in a very thin dextran layer of a few nanometers.
27455406	10	91	theme	aqueous	1953:1959	arg1	environments					1961:1972	aqueous environments	1953:1972	aqueous environments using present instrumentations and highly hydrated surface layers	1953:2038	The developed methodologies allowed to design and fabricate nanometer scale CMD layers with well-controlled surface structure, which are very difficult to characterize in aqueous environments using present instrumentations and highly hydrated surface layers.
25492055	2	0	theme	hemolysis	602:610	arg1	%					612:612	hemolysis %<5	602:614	hemolysis %<5	602:614	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	5	1	theme	prolonged	1076:1084	arg1	release					1091:1097	its prolonged drug release	1072:1097	its prolonged drug release	1072:1097	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	4	2	theme	compressive	862:872	arg1	modulus					874:880	compressive modulus	862:880	compressive modulus than other fabricated scaffolds	862:912	Double-hybrid silk fibroin/chitosan-blended scaffold shows higher compressive strength and compressive modulus than other fabricated scaffolds.
25492055	2	3	theme	fabricated	437:446	arg1	systems					463:469	fabricated amenable model systems	437:469	fabricated amenable model systems	437:469	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	5	4	theme	comparative	917:927	arg1	profile					942:948	A comparative drug release profile	915:948	A comparative drug release profile of fabricated scaffolds	915:972	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	1	5	theme	silk	296:299	arg1	fibroin					301:307	double-hybrid silk fibroin	282:307	double-hybrid silk fibroin	282:307	In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds.
25492055	0	6	theme	drug	110:113	arg1	administration					115:128	drug administration	110:128	drug administration	110:128	Chitosan silk-based three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility.
25492055	5	7	theme	fabricated	953:962	arg1	scaffolds					964:972	fabricated scaffolds	953:972	fabricated scaffolds	953:972	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	0	8	theme	blood	134:138	arg1	compatibility					140:152	blood compatibility	134:152	blood compatibility	134:152	Chitosan silk-based three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility.
25492055	5	9	theme	drug	1086:1089	arg1	release					1091:1097	its prolonged drug release	1072:1097	its prolonged drug release	1072:1097	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	5	10	theme	pertinent	1038:1046	arg1	system					1054:1059	a pertinent model system	1036:1059	a pertinent model system	1036:1059	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	5	10	theme	pertinent	1038:1046	arg1	scaffold					1024:1031	double-hybrid silk fibroin/chitosan scaffold	988:1031	double-hybrid silk fibroin/chitosan scaffold	988:1031	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	5	11	theme	silk	1002:1005	arg1	system					1054:1059	a pertinent model system	1036:1059	a pertinent model system	1036:1059	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	5	11	theme	silk	1002:1005	arg1	scaffold					1024:1031	double-hybrid silk fibroin/chitosan scaffold	988:1031	double-hybrid silk fibroin/chitosan scaffold	988:1031	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	0	12	contain	containing	48:57	arg2	beads					100:104	gentamicin-encapsulated calcium alginate beads	59:104	gentamicin-encapsulated calcium alginate beads	59:104	Chitosan silk-based three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility.
25492055	0	12	contain	containing	48:57	arg1	scaffolds					38:46	three-dimensional scaffolds	20:46	three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility	20:152	Chitosan silk-based three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility.
25492055	3	13	theme	infrared	653:660	arg1	results					662:668	infrared results	653:668	infrared results	653:668	Fourier transform infrared results confirmed the blend formation and scanning electron microscope images showed good interconnectivity.
25492055	3	14	theme	blend	684:688	arg1	formation					690:698	the blend formation	680:698	the blend formation	680:698	Fourier transform infrared results confirmed the blend formation and scanning electron microscope images showed good interconnectivity.
25492055	2	15	theme	porosity	620:627	arg1	%					632:632	porosity >85%	620:632	porosity >85%	620:632	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	5	16	theme	release	934:940	arg1	profile					942:948	A comparative drug release profile	915:948	A comparative drug release profile of fabricated scaffolds	915:972	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	4	17	theme	higher	830:835	arg1	strength					849:856	higher compressive strength	830:856	higher compressive strength	830:856	Double-hybrid silk fibroin/chitosan-blended scaffold shows higher compressive strength and compressive modulus than other fabricated scaffolds.
25492055	1	18	theme	calcium	211:217	arg1	beads					228:232	calcium alginate beads	211:232	calcium alginate beads	211:232	In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds.
25492055	0	19	theme	calcium	83:89	arg1	beads					100:104	gentamicin-encapsulated calcium alginate beads	59:104	gentamicin-encapsulated calcium alginate beads	59:104	Chitosan silk-based three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility.
25492055	2	20	theme	scaffolds	589:597	arg1	nature					512:517	hemocompatible nature	497:517	hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%	497:632	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	3	21	dep	Fourier	635:641	arg1	transform					643:651	transform	643:651	transform infrared results	643:668	Fourier transform infrared results confirmed the blend formation and scanning electron microscope images showed good interconnectivity.
25492055	1	22	theme	alginate	219:226	arg1	beads					228:232	calcium alginate beads	211:232	calcium alginate beads	211:232	In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds.
25492055	5	23	theme	double-hybrid	988:1000	arg1	system					1054:1059	a pertinent model system	1036:1059	a pertinent model system	1036:1059	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	5	23	theme	double-hybrid	988:1000	arg1	scaffold					1024:1031	double-hybrid silk fibroin/chitosan scaffold	988:1031	double-hybrid silk fibroin/chitosan scaffold	988:1031	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	5	24	theme	compressive	1135:1145	arg1	modulus					1147:1153	high compressive modulus	1130:1153	high compressive modulus	1130:1153	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	4	25	theme	silk	785:788	arg1	scaffold					815:822	Double-hybrid silk fibroin/chitosan-blended scaffold	771:822	Double-hybrid silk fibroin/chitosan-blended scaffold	771:822	Double-hybrid silk fibroin/chitosan-blended scaffold shows higher compressive strength and compressive modulus than other fabricated scaffolds.
25492055	2	26	theme	fibroin/chitosan	541:556	arg1	nature					512:517	hemocompatible nature	497:517	hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%	497:632	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	4	27	theme	other	887:891	arg1	scaffolds					904:912	other fabricated scaffolds	887:912	other fabricated scaffolds	887:912	Double-hybrid silk fibroin/chitosan-blended scaffold shows higher compressive strength and compressive modulus than other fabricated scaffolds.
25492055	2	28	theme	Physiochemical	378:391	arg1	properties					423:432	Physiochemical, morphological and biological properties	378:432	properties	423:432	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	2	29	theme	systems	463:469	arg1	properties					423:432	Physiochemical, morphological and biological properties	378:432	properties	423:432	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	2	30	theme	silk	536:539	arg1	fibroin/chitosan					541:556	double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds	522:597	fibroin/chitosan	541:556	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	1	31	theme	double-hybrid	331:343	arg1	scaffolds					367:375	double-hybrid silk fibroin/chitosan scaffolds	331:375	double-hybrid silk fibroin/chitosan scaffolds	331:375	In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds.
25492055	5	32	theme	high	1130:1133	arg1	modulus					1147:1153	high compressive modulus	1130:1153	high compressive modulus	1130:1153	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	2	33	theme	double-hybrid	522:534	arg1	fibroin/chitosan					541:556	double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds	522:597	fibroin/chitosan	541:556	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	1	34	theme	silk	345:348	arg1	scaffolds					367:375	double-hybrid silk fibroin/chitosan scaffolds	331:375	double-hybrid silk fibroin/chitosan scaffolds	331:375	In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds.
25492055	5	35	theme	fibroin/chitosan	1007:1022	arg1	system					1054:1059	a pertinent model system	1036:1059	a pertinent model system	1036:1059	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	5	35	theme	fibroin/chitosan	1007:1022	arg1	scaffold					1024:1031	double-hybrid silk fibroin/chitosan scaffold	988:1031	double-hybrid silk fibroin/chitosan scaffold	988:1031	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	0	36	theme	gentamicin-encapsulated	59:81	arg1	beads					100:104	gentamicin-encapsulated calcium alginate beads	59:104	gentamicin-encapsulated calcium alginate beads	59:104	Chitosan silk-based three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility.
25492055	1	37	theme	fibroin/chitosan	350:365	arg1	scaffolds					367:375	double-hybrid silk fibroin/chitosan scaffolds	331:375	double-hybrid silk fibroin/chitosan scaffolds	331:375	In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds.
25492055	2	38	theme	hemocompatible	497:510	arg1	nature					512:517	hemocompatible nature	497:517	hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%	497:632	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	3	39	theme	scanning	704:711	arg1	images					733:738	scanning electron microscope images	704:738	scanning electron microscope images	704:738	Fourier transform infrared results confirmed the blend formation and scanning electron microscope images showed good interconnectivity.
25492055	1	40	theme	porous	256:261	arg1	chitosan					263:270	porous chitosan	256:270	porous chitosan	256:270	In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds.
25492055	2	41	theme	fibroin	581:587	arg1	scaffolds					589:597	double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds	522:597	scaffolds	589:597	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	5	42	theme	scaffolds	964:972	arg1	profile					942:948	A comparative drug release profile	915:948	A comparative drug release profile of fabricated scaffolds	915:972	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	3	43	theme	electron	713:720	arg1	images					733:738	scanning electron microscope images	704:738	scanning electron microscope images	704:738	Fourier transform infrared results confirmed the blend formation and scanning electron microscope images showed good interconnectivity.
25492055	4	44	theme	Double-hybrid	771:783	arg1	scaffold					815:822	Double-hybrid silk fibroin/chitosan-blended scaffold	771:822	Double-hybrid silk fibroin/chitosan-blended scaffold	771:822	Double-hybrid silk fibroin/chitosan-blended scaffold shows higher compressive strength and compressive modulus than other fabricated scaffolds.
25492055	2	45	theme	silk	576:579	arg1	scaffolds					589:597	double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds	522:597	scaffolds	589:597	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	2	46	theme	%	632:632	arg1	nature					512:517	hemocompatible nature	497:517	hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%	497:632	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	2	47	theme	%	612:612	arg1	nature					512:517	hemocompatible nature	497:517	hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%	497:632	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	5	48	theme	optimal	1100:1106	arg1	hemocompatability					1108:1124	optimal hemocompatability	1100:1124	optimal hemocompatability	1100:1124	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	2	49	theme	double-hybrid	562:574	arg1	scaffolds					589:597	double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds	522:597	scaffolds	589:597	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	2	50	theme	biological	412:421	arg1	properties					423:432	Physiochemical, morphological and biological properties	378:432	properties	423:432	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	4	51	theme	compressive	837:847	arg1	strength					849:856	higher compressive strength	830:856	higher compressive strength	830:856	Double-hybrid silk fibroin/chitosan-blended scaffold shows higher compressive strength and compressive modulus than other fabricated scaffolds.
25492055	1	52	theme	present	162:168	arg1	study					170:174	the present study	158:174	the present study	158:174	In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds.
25492055	3	53	theme	good	747:750	arg1	interconnectivity					752:768	good interconnectivity	747:768	good interconnectivity	747:768	Fourier transform infrared results confirmed the blend formation and scanning electron microscope images showed good interconnectivity.
25492055	2	54	theme	morphological	394:406	arg1	properties					423:432	Physiochemical, morphological and biological properties	378:432	properties	423:432	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	4	55	theme	fabricated	893:902	arg1	scaffolds					904:912	other fabricated scaffolds	887:912	other fabricated scaffolds	887:912	Double-hybrid silk fibroin/chitosan-blended scaffold shows higher compressive strength and compressive modulus than other fabricated scaffolds.
25492055	5	56	theme	drug	929:932	arg1	profile					942:948	A comparative drug release profile	915:948	A comparative drug release profile of fabricated scaffolds	915:972	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	5	57	theme	model	1048:1052	arg1	system					1054:1059	a pertinent model system	1036:1059	a pertinent model system	1036:1059	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	5	57	theme	model	1048:1052	arg1	scaffold					1024:1031	double-hybrid silk fibroin/chitosan scaffold	988:1031	double-hybrid silk fibroin/chitosan scaffold	988:1031	A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
25492055	3	58	theme	microscope	722:731	arg1	images					733:738	scanning electron microscope images	704:738	scanning electron microscope images	704:738	Fourier transform infrared results confirmed the blend formation and scanning electron microscope images showed good interconnectivity.
25492055	2	59	theme	model	457:461	arg1	systems					463:469	fabricated amenable model systems	437:469	fabricated amenable model systems	437:469	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	4	60	theme	fibroin/chitosan-blended	790:813	arg1	scaffold					815:822	Double-hybrid silk fibroin/chitosan-blended scaffold	771:822	Double-hybrid silk fibroin/chitosan-blended scaffold	771:822	Double-hybrid silk fibroin/chitosan-blended scaffold shows higher compressive strength and compressive modulus than other fabricated scaffolds.
25492055	0	61	theme	alginate	91:98	arg1	beads					100:104	gentamicin-encapsulated calcium alginate beads	59:104	gentamicin-encapsulated calcium alginate beads	59:104	Chitosan silk-based three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility.
25492055	0	62	theme	three-dimensional	20:36	arg1	scaffolds					38:46	three-dimensional scaffolds	20:46	three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility	20:152	Chitosan silk-based three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility.
25492055	2	63	theme	amenable	448:455	arg1	systems					463:469	fabricated amenable model systems	437:469	fabricated amenable model systems	437:469	Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%.
25492055	1	64	theme	double-hybrid	282:294	arg1	fibroin					301:307	double-hybrid silk fibroin	282:307	double-hybrid silk fibroin	282:307	In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds.
28104367	6	0	theme	antimicrobial	864:876	arg1	related					901:907	related	901:907	related	901:907	Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite.
28104367	6	0	theme	antimicrobial	864:876	arg1	efficiency					878:887	the antimicrobial efficiency	860:887	the antimicrobial efficiency	860:887	Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite.
28104367	5	1	theme	packed	709:714	arg1	meat					716:719	packed meat	709:719	packed meat	709:719	Two bacterial strains that commonly contaminate in packed meat were selected as target microbes to elucidate the antimicrobial activity of the prepared C-ZnO film.
28104367	7	2	theme	packaging	1044:1052	arg1	pouches					1054:1060	packaging pouches	1044:1060	packaging pouches for raw meat	1044:1073	The C-2 films exhibited excellent antimicrobial activity and was fabricated into packaging pouches for raw meat.
28104367	3	3	theme	C-ZnC	452:456	arg1	films					458:462	C-ZnC films	452:462	C-ZnC films	452:462	In order to tune the property of C-ZnC films, four different composite films were prepared by varying the concentration of ZnO.
28104367	1	4	theme	first	224:228	arg1	report					230:235	the first report	220:235	the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging	220:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	1	4	theme	first	224:228	arg1	breakthrough					114:125	a breakthrough	112:125	a breakthrough to open up the industrial use of novel environmentally benign packaging material	112:206	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	1	5	theme	smart	322:326	arg1	packaging					328:336	smart packaging	322:336	smart packaging	322:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	3	6	theme	composite	480:488	arg1	films					490:494	four different composite films	465:494	four different composite films	465:494	In order to tune the property of C-ZnC films, four different composite films were prepared by varying the concentration of ZnO.
28104367	9	7	theme	raw	1356:1358	arg1	meat					1360:1363	raw meat	1356:1363	raw meat	1356:1363	The C-2 pouches stand as a top-notch material when compared to polyethylene bag in extending the shelf life of raw meat.
28104367	0	8	theme	shelf	85:89	arg1	life					91:94	the shelf life	81:94	the shelf life of raw meat	81:106	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.
28104367	9	9	theme	C-2	1249:1251	arg1	pouches					1253:1259	The C-2 pouches	1245:1259	The C-2 pouches	1245:1259	The C-2 pouches stand as a top-notch material when compared to polyethylene bag in extending the shelf life of raw meat.
28104367	8	10	from	4°C	1240:1242	arg1	day					1222:1224	the sixth day	1212:1224	the sixth day of storage at 4°C	1212:1242	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	8	10	from	4°C	1240:1242	arg1	storage					1229:1235	storage	1229:1235	storage at 4°C	1229:1242	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	6	11	theme	nano-particles	930:943	arg1	amount					916:921	the amount	912:921	the amount of ZnO nano-particles in the composite	912:960	Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite.
28104367	6	11	theme	nano-particles	930:943	arg1	nano-particles					930:943	ZnO nano-particles	926:943	ZnO nano-particles in the composite	926:960	Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite.
28104367	0	12	theme	raw	99:101	arg1	meat					103:106	raw meat	99:106	raw meat	99:106	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.
28104367	5	13	theme	antimicrobial	771:783	arg1	activity					785:792	the antimicrobial activity	767:792	the antimicrobial activity of the prepared C-ZnO film	767:819	Two bacterial strains that commonly contaminate in packed meat were selected as target microbes to elucidate the antimicrobial activity of the prepared C-ZnO film.
28104367	5	14	theme	target	738:743	arg1	strains					672:678	Two bacterial strains	658:678	Two bacterial strains that commonly contaminate in packed meat	658:719	Two bacterial strains that commonly contaminate in packed meat were selected as target microbes to elucidate the antimicrobial activity of the prepared C-ZnO film.
28104367	5	14	theme	target	738:743	arg1	microbes					745:752	target microbes	738:752	target microbes	738:752	Two bacterial strains that commonly contaminate in packed meat were selected as target microbes to elucidate the antimicrobial activity of the prepared C-ZnO film.
28104367	9	15	theme	meat	1360:1363	arg1	life					1348:1351	the shelf life	1338:1351	the shelf life of raw meat	1338:1363	The C-2 pouches stand as a top-notch material when compared to polyethylene bag in extending the shelf life of raw meat.
28104367	8	16	theme	growth	1202:1207	arg1	inhibition					1178:1187	its complete inhibition	1165:1187	its complete inhibition of microbial growth	1165:1207	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	2	17	theme	films	412:416	arg1	preparation					387:397	the preparation	383:397	the preparation of the C-ZnC films	383:416	A facile, one pot procedure was adopted for the preparation of the C-ZnC films.
28104367	6	18	theme	Detailed	822:829	arg1	investigations					831:844	Detailed investigations	822:844	Detailed investigations	822:844	Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite.
28104367	6	19	from	nano-particles	930:943	arg1	composite					952:960	the composite	948:960	the composite	948:960	Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite.
28104367	3	20	theme	different	470:478	arg1	films					490:494	four different composite films	465:494	four different composite films	465:494	In order to tune the property of C-ZnC films, four different composite films were prepared by varying the concentration of ZnO.
28104367	1	21	theme	industrial	142:151	arg1	use					153:155	the industrial use	138:155	the industrial use of novel environmentally benign packaging material	138:206	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	0	22	theme	chitosan-nano	9:21	arg1	pouches					41:47	Flexible chitosan-nano ZnO antimicrobial pouches	0:47	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.	0:107	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.
28104367	8	23	theme	significant	1104:1114	arg1	action					1116:1121	significant action	1104:1121	significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C	1104:1242	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	6	24	from	composite	952:960	arg1	amount					916:921	the amount	912:921	the amount of ZnO nano-particles in the composite	912:960	Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite.
28104367	6	24	from	composite	952:960	arg1	nano-particles					930:943	ZnO nano-particles	926:943	ZnO nano-particles in the composite	926:960	Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite.
28104367	6	25	theme	ZnO	926:928	arg1	nano-particles					930:943	ZnO nano-particles	926:943	ZnO nano-particles in the composite	926:960	Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite.
28104367	5	26	theme	C-ZnO	810:814	arg1	film					816:819	the prepared C-ZnO film	797:819	the prepared C-ZnO film	797:819	Two bacterial strains that commonly contaminate in packed meat were selected as target microbes to elucidate the antimicrobial activity of the prepared C-ZnO film.
28104367	0	27	theme	Flexible	0:7	arg1	pouches					41:47	Flexible chitosan-nano ZnO antimicrobial pouches	0:47	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.	0:107	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.
28104367	1	28	theme	portable	240:247	arg1	pouches					277:283	portable chitosan-ZnO nano-composite pouches	240:283	portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging	240:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	1	28	theme	portable	240:247	arg1	entrants					310:317	elite entrants	304:317	elite entrants in smart packaging	304:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	5	29	theme	prepared	801:808	arg1	film					816:819	the prepared C-ZnO film	797:819	the prepared C-ZnO film	797:819	Two bacterial strains that commonly contaminate in packed meat were selected as target microbes to elucidate the antimicrobial activity of the prepared C-ZnO film.
28104367	8	30	theme	sixth	1216:1220	arg1	day					1222:1224	the sixth day	1212:1224	the sixth day of storage at 4°C	1212:1242	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	5	31	theme	film	816:819	arg1	activity					785:792	the antimicrobial activity	767:792	the antimicrobial activity of the prepared C-ZnO film	767:819	Two bacterial strains that commonly contaminate in packed meat were selected as target microbes to elucidate the antimicrobial activity of the prepared C-ZnO film.
28104367	0	32	theme	antimicrobial	27:39	arg1	pouches					41:47	Flexible chitosan-nano ZnO antimicrobial pouches	0:47	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.	0:107	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.
28104367	1	33	theme	chitosan-ZnO	249:260	arg1	pouches					277:283	portable chitosan-ZnO nano-composite pouches	240:283	portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging	240:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	1	33	theme	chitosan-ZnO	249:260	arg1	entrants					310:317	elite entrants	304:317	elite entrants in smart packaging	304:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	1	34	from	report	230:235	arg1	pouches					277:283	portable chitosan-ZnO nano-composite pouches	240:283	portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging	240:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	1	34	from	report	230:235	arg1	entrants					310:317	elite entrants	304:317	elite entrants in smart packaging	304:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	1	35	theme	novel	160:164	arg1	material					199:206	novel environmentally benign packaging material	160:206	novel environmentally benign packaging material	160:206	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	0	36	theme	ZnO	23:25	arg1	pouches					41:47	Flexible chitosan-nano ZnO antimicrobial pouches	0:47	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.	0:107	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.
28104367	1	37	theme	nano-composite	262:275	arg1	pouches					277:283	portable chitosan-ZnO nano-composite pouches	240:283	portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging	240:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	1	37	theme	nano-composite	262:275	arg1	entrants					310:317	elite entrants	304:317	elite entrants in smart packaging	304:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	0	38	theme	meat	103:106	arg1	life					91:94	the shelf life	81:94	the shelf life of raw meat	81:106	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.
28104367	8	39	from	action	1116:1121	arg1	day					1222:1224	the sixth day	1212:1224	the sixth day of storage at 4°C	1212:1242	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	3	40	theme	films	458:462	arg1	property					440:447	the property	436:447	the property of C-ZnC films	436:462	In order to tune the property of C-ZnC films, four different composite films were prepared by varying the concentration of ZnO.
28104367	8	41	theme	complete	1169:1176	arg1	inhibition					1178:1187	its complete inhibition	1165:1187	its complete inhibition of microbial growth	1165:1207	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	8	42	theme	storage	1229:1235	arg1	day					1222:1224	the sixth day	1212:1224	the sixth day of storage at 4°C	1212:1242	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	4	43	theme	bare	614:617	arg1	chitosan					619:626	chitosan	619:626	chitosan	619:626	The prepared films were found to be much superior when compared to bare chitosan and other conventional films.
28104367	5	44	theme	bacterial	662:670	arg1	strains					672:678	Two bacterial strains	658:678	Two bacterial strains that commonly contaminate in packed meat	658:719	Two bacterial strains that commonly contaminate in packed meat were selected as target microbes to elucidate the antimicrobial activity of the prepared C-ZnO film.
28104367	5	44	theme	bacterial	662:670	arg1	microbes					745:752	target microbes	738:752	target microbes	738:752	Two bacterial strains that commonly contaminate in packed meat were selected as target microbes to elucidate the antimicrobial activity of the prepared C-ZnO film.
28104367	3	45	theme	ZnO	542:544	arg1	concentration					525:537	the concentration	521:537	the concentration of ZnO	521:544	In order to tune the property of C-ZnC films, four different composite films were prepared by varying the concentration of ZnO.
28104367	7	46	theme	C-2	967:969	arg1	films					971:975	The C-2 films	963:975	The C-2 films	963:975	The C-2 films exhibited excellent antimicrobial activity and was fabricated into packaging pouches for raw meat.
28104367	1	47	theme	packaging	189:197	arg1	material					199:206	novel environmentally benign packaging material	160:206	novel environmentally benign packaging material	160:206	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	0	48	theme	new	54:56	arg1	material					58:65	a new material	52:65	a new material for extending the shelf life of raw meat	52:106	Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.
28104367	7	49	theme	antimicrobial	997:1009	arg1	activity					1011:1018	excellent antimicrobial activity	987:1018	excellent antimicrobial activity	987:1018	The C-2 films exhibited excellent antimicrobial activity and was fabricated into packaging pouches for raw meat.
28104367	9	50	theme	top-notch	1272:1280	arg1	material					1282:1289	a top-notch material	1270:1289	a top-notch material	1270:1289	The C-2 pouches stand as a top-notch material when compared to polyethylene bag in extending the shelf life of raw meat.
28104367	4	51	theme	conventional	638:649	arg1	films					651:655	other conventional films	632:655	other conventional films	632:655	The prepared films were found to be much superior when compared to bare chitosan and other conventional films.
28104367	1	52	from	entrants	310:317	arg1	packaging					328:336	smart packaging	322:336	smart packaging	322:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	9	53	theme	polyethylene	1308:1319	arg1	bag					1321:1323	polyethylene bag	1308:1323	polyethylene bag in extending the shelf life of raw meat	1308:1363	The C-2 pouches stand as a top-notch material when compared to polyethylene bag in extending the shelf life of raw meat.
28104367	6	54	from	amount	916:921	arg1	composite					952:960	the composite	948:960	the composite	948:960	Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite.
28104367	7	55	theme	raw	1066:1068	arg1	meat					1070:1073	raw meat	1066:1073	raw meat	1066:1073	The C-2 films exhibited excellent antimicrobial activity and was fabricated into packaging pouches for raw meat.
28104367	4	56	theme	other	632:636	arg1	films					651:655	other conventional films	632:655	other conventional films	632:655	The prepared films were found to be much superior when compared to bare chitosan and other conventional films.
28104367	8	57	theme	prepared	1080:1087	arg1	pouches					1089:1095	The prepared pouches	1076:1095	The prepared pouches	1076:1095	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	8	58	from	microbes	1135:1142	arg1	meat					1151:1154	raw meat	1147:1154	raw meat owing to its complete inhibition of microbial growth	1147:1207	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	1	59	theme	material	199:206	arg1	use					153:155	the industrial use	138:155	the industrial use of novel environmentally benign packaging material	138:206	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	7	60	theme	excellent	987:995	arg1	activity					1011:1018	excellent antimicrobial activity	987:1018	excellent antimicrobial activity	987:1018	The C-2 films exhibited excellent antimicrobial activity and was fabricated into packaging pouches for raw meat.
28104367	8	61	theme	microbial	1192:1200	arg1	growth					1202:1207	microbial growth	1192:1207	microbial growth	1192:1207	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	8	62	theme	raw	1147:1149	arg1	meat					1151:1154	raw meat	1147:1154	raw meat owing to its complete inhibition of microbial growth	1147:1207	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	1	63	dep	novel	160:164	arg1	benign					182:187	benign	182:187	benign	182:187	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	2	64	theme	facile	341:346	arg1	procedure					357:365	A facile, one pot procedure	339:365	procedure	357:365	A facile, one pot procedure was adopted for the preparation of the C-ZnC films.
28104367	2	65	theme	pot	353:355	arg1	procedure					357:365	A facile, one pot procedure	339:365	procedure	357:365	A facile, one pot procedure was adopted for the preparation of the C-ZnC films.
28104367	1	66	theme	elite	304:308	arg1	pouches					277:283	portable chitosan-ZnO nano-composite pouches	240:283	portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging	240:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	1	66	theme	elite	304:308	arg1	entrants					310:317	elite entrants	304:317	elite entrants in smart packaging	304:336	As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging.
28104367	9	67	theme	shelf	1342:1346	arg1	life					1348:1351	the shelf life	1338:1351	the shelf life of raw meat	1338:1363	The C-2 pouches stand as a top-notch material when compared to polyethylene bag in extending the shelf life of raw meat.
28104367	8	68	from	day	1222:1224	arg1	4°C					1240:1242	4°C	1240:1242	4°C	1240:1242	The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C.
28104367	2	69	theme	C-ZnC	406:410	arg1	films					412:416	the C-ZnC films	402:416	the C-ZnC films	402:416	A facile, one pot procedure was adopted for the preparation of the C-ZnC films.
28104367	4	70	theme	prepared	551:558	arg1	superior					588:595	superior	588:595	superior	588:595	The prepared films were found to be much superior when compared to bare chitosan and other conventional films.
28104367	4	70	theme	prepared	551:558	arg1	films					560:564	The prepared films	547:564	The prepared films	547:564	The prepared films were found to be much superior when compared to bare chitosan and other conventional films.
24642967	8	0	theme	S.	1272:1273	arg1	mechanisms					1290:1299	S. suis virulence mechanisms	1272:1299	S. suis virulence mechanisms	1272:1299	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	4	1	theme	profound	466:473	arg1	effect					475:480	a profound effect	464:480	a profound effect of carbohydrate availability on the expression of virulence genes	464:546	In the oropharyngeal cavity, where glucose is rapidly absorbed but dietary α-glucans persist, there is a profound effect of carbohydrate availability on the expression of virulence genes.
24642967	8	2	theme	strategies	1420:1429	arg1	development					1390:1400	the development	1386:1400	the development of anti-infective strategies	1386:1429	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	1	3	theme	economic	166:173	arg1	problems					175:182	worldwide economic problems	156:182	worldwide economic problems for the pig industry	156:203	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	4	4	theme	carbohydrate	485:496	arg1	availability					498:509	carbohydrate availability	485:509	carbohydrate availability	485:509	In the oropharyngeal cavity, where glucose is rapidly absorbed but dietary α-glucans persist, there is a profound effect of carbohydrate availability on the expression of virulence genes.
24642967	5	5	theme	S.	581:582	arg1	genes					599:603	S. suis virulence genes	581:603	S. suis virulence genes that promote adhesion to and invasion of epithelial cells	581:661	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	6	6	theme	suilysin	835:842	arg1	production					821:830	the production	817:830	the production of suilysin, a toxin that damages epithelial cells,	817:882	Additionally the production of suilysin, a toxin that damages epithelial cells, was increased more than ten-fold when glucose levels were low and S. suis was growing on pullulan.
24642967	5	7	dep	Nineteen	549:556	arg1	confirmed					571:579	confirmed	571:579	confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells	571:661	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	5	7	dep	Nineteen	549:556	arg1	predicted					558:566	predicted	558:566	predicted	558:566	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	8	8	theme	anti-infective	1405:1418	arg1	strategies					1420:1429	anti-infective strategies	1405:1429	anti-infective strategies	1405:1429	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	7	9	theme	repression	1150:1159	arg1	effect					1122:1127	the effect	1118:1127	the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood	1118:1224	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	4	10	from	effect	475:480	arg1	expression					518:527	the expression	514:527	the expression of virulence genes	514:546	In the oropharyngeal cavity, where glucose is rapidly absorbed but dietary α-glucans persist, there is a profound effect of carbohydrate availability on the expression of virulence genes.
24642967	4	11	theme	oropharyngeal	368:380	arg1	cavity					382:387	the oropharyngeal cavity	364:387	the oropharyngeal cavity	364:387	In the oropharyngeal cavity, where glucose is rapidly absorbed but dietary α-glucans persist, there is a profound effect of carbohydrate availability on the expression of virulence genes.
24642967	8	12	theme	mechanisms	1290:1299	arg1	understanding					1255:1267	our understanding	1251:1267	our understanding of S. suis virulence mechanisms	1251:1299	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	8	13	theme	animal	1445:1450	arg1	composition					1457:1467	animal feed composition	1445:1467	animal feed composition	1445:1467	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	3	14	theme	invasive	343:350	arg1	disease					352:358	invasive disease	343:358	invasive disease	343:358	Colonization of porcine oropharynx by S. suis is considered to be a high risk factor for invasive disease.
24642967	5	15	theme	epithelial	646:655	arg1	cells					657:661	epithelial cells	646:661	epithelial cells	646:661	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	5	16	theme	virulence	589:597	arg1	genes					599:603	S. suis virulence genes	581:603	S. suis virulence genes that promote adhesion to and invasion of epithelial cells	581:661	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	8	17	theme	virulence	1280:1288	arg1	mechanisms					1290:1299	S. suis virulence mechanisms	1272:1299	S. suis virulence mechanisms	1272:1299	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	3	18	theme	risk	327:330	arg1	factor					332:337	a high risk factor	320:337	a high risk factor for invasive disease	320:358	Colonization of porcine oropharynx by S. suis is considered to be a high risk factor for invasive disease.
24642967	3	18	theme	risk	327:330	arg1	Colonization					254:265	Colonization	254:265	Colonization of porcine oropharynx by S. suis	254:298	Colonization of porcine oropharynx by S. suis is considered to be a high risk factor for invasive disease.
24642967	6	19	theme	epithelial	866:875	arg1	cells					877:881	epithelial cells	866:881	epithelial cells	866:881	Additionally the production of suilysin, a toxin that damages epithelial cells, was increased more than ten-fold when glucose levels were low and S. suis was growing on pullulan.
24642967	7	20	theme	gene	1045:1048	arg1	studies					1061:1067	in vitro and in vivo gene expression studies	1024:1067	in vitro and in vivo gene expression studies	1024:1067	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	1	21	theme	Streptococcus	85:97	arg1	pathogen					125:132	a major bacterial pathogen	107:132	a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry	107:203	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	1	21	theme	Streptococcus	85:97	arg1	suis					99:102	Streptococcus suis	85:102	Streptococcus suis	85:102	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	7	22	theme	catabolite	1139:1148	arg1	repression					1150:1159	carbon catabolite repression	1132:1159	carbon catabolite repression	1132:1159	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	0	23	from	expression	51:60	arg1	suis					79:82	Streptococcus suis	65:82	Streptococcus suis	65:82	Carbohydrate availability regulates virulence gene expression in Streptococcus suis.
24642967	7	24	theme	expression	1050:1059	arg1	studies					1061:1067	in vitro and in vivo gene expression studies	1024:1067	in vitro and in vivo gene expression studies	1024:1067	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	5	25	theme	carbon	789:794	arg1	suis					703:706	S. suis	700:706	S. suis	700:706	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	5	25	theme	carbon	789:794	arg1	source					796:801	the single carbon source	778:801	the single carbon source	778:801	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	7	26	theme	carbon	1132:1137	arg1	repression					1150:1159	carbon catabolite repression	1132:1159	carbon catabolite repression	1132:1159	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	5	27	theme	single	782:787	arg1	suis					703:706	S. suis	700:706	S. suis	700:706	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	5	27	theme	single	782:787	arg1	source					796:801	the single carbon source	778:801	the single carbon source	778:801	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	7	28	theme	in	1037:1038	arg1	studies					1061:1067	in vitro and in vivo gene expression studies	1024:1067	in vitro and in vivo gene expression studies	1024:1067	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	1	29	theme	pig	192:194	arg1	industry					196:203	the pig industry	188:203	the pig industry	188:203	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	0	30	theme	Carbohydrate	0:11	arg1	availability					13:24	Carbohydrate availability	0:24	Carbohydrate availability	0:24	Carbohydrate availability regulates virulence gene expression in Streptococcus suis.
24642967	4	31	theme	virulence	532:540	arg1	genes					542:546	virulence genes	532:546	virulence genes	532:546	In the oropharyngeal cavity, where glucose is rapidly absorbed but dietary α-glucans persist, there is a profound effect of carbohydrate availability on the expression of virulence genes.
24642967	8	32	theme	important	1309:1317	arg1	implications					1319:1330	important implications	1309:1330	important implications for the design of future control strategies including the development of anti-infective strategies	1309:1429	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	0	33	theme	virulence	36:44	arg1	expression					51:60	virulence gene expression	36:60	virulence gene expression in Streptococcus suis	36:82	Carbohydrate availability regulates virulence gene expression in Streptococcus suis.
24642967	3	34	theme	oropharynx	278:287	arg1	factor					332:337	a high risk factor	320:337	a high risk factor for invasive disease	320:358	Colonization of porcine oropharynx by S. suis is considered to be a high risk factor for invasive disease.
24642967	3	34	theme	oropharynx	278:287	arg1	Colonization					254:265	Colonization	254:265	Colonization of porcine oropharynx by S. suis	254:298	Colonization of porcine oropharynx by S. suis is considered to be a high risk factor for invasive disease.
24642967	1	35	theme	major	109:113	arg1	pathogen					125:132	a major bacterial pathogen	107:132	a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry	107:203	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	1	35	theme	major	109:113	arg1	suis					99:102	Streptococcus suis	85:102	Streptococcus suis	85:102	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	4	36	theme	dietary	428:434	arg1	α-glucans					436:444	dietary α-glucans	428:444	dietary α-glucans	428:444	In the oropharyngeal cavity, where glucose is rapidly absorbed but dietary α-glucans persist, there is a profound effect of carbohydrate availability on the expression of virulence genes.
24642967	1	37	theme	bacterial	115:123	arg1	pathogen					125:132	a major bacterial pathogen	107:132	a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry	107:203	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	1	37	theme	bacterial	115:123	arg1	suis					99:102	Streptococcus suis	85:102	Streptococcus suis	85:102	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	7	38	dep	in	1037:1038	arg1	vivo					1040:1043	vivo	1040:1043	vivo	1040:1043	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	4	39	theme	genes	542:546	arg1	expression					518:527	the expression	514:527	the expression of virulence genes	514:546	In the oropharyngeal cavity, where glucose is rapidly absorbed but dietary α-glucans persist, there is a profound effect of carbohydrate availability on the expression of virulence genes.
24642967	0	40	theme	gene	46:49	arg1	expression					51:60	virulence gene expression	36:60	virulence gene expression in Streptococcus suis	36:82	Carbohydrate availability regulates virulence gene expression in Streptococcus suis.
24642967	6	41	theme	glucose	922:928	arg1	levels					930:935	glucose levels	922:935	glucose levels	922:935	Additionally the production of suilysin, a toxin that damages epithelial cells, was increased more than ten-fold when glucose levels were low and S. suis was growing on pullulan.
24642967	3	42	theme	porcine	270:276	arg1	oropharynx					278:287	porcine oropharynx	270:287	porcine oropharynx	270:287	Colonization of porcine oropharynx by S. suis is considered to be a high risk factor for invasive disease.
24642967	2	43	theme	emerging	225:232	arg1	pathogen					234:241	an emerging pathogen	222:241	an emerging pathogen of humans	222:251	S. suis is also an emerging pathogen of humans.
24642967	2	43	theme	emerging	225:232	arg1	suis					209:212	S. suis	206:212	S. suis	206:212	S. suis is also an emerging pathogen of humans.
24642967	7	44	dep	in	1024:1025	arg1	vitro					1027:1031	vitro	1027:1031	vitro	1027:1031	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	0	45	theme	Streptococcus	65:77	arg1	suis					79:82	Streptococcus suis	65:82	Streptococcus suis	65:82	Carbohydrate availability regulates virulence gene expression in Streptococcus suis.
24642967	4	46	theme	availability	498:509	arg1	effect					475:480	a profound effect	464:480	a profound effect of carbohydrate availability on the expression of virulence genes	464:546	In the oropharyngeal cavity, where glucose is rapidly absorbed but dietary α-glucans persist, there is a profound effect of carbohydrate availability on the expression of virulence genes.
24642967	5	47	theme	cells	657:661	arg1	adhesion					618:625	adhesion	618:625	adhesion to	618:628	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	5	47	theme	cells	657:661	arg1	invasion					634:641	invasion	634:641	invasion of epithelial cells	634:661	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	5	48	theme	α-glucan	730:737	arg1	starch/pullulan					739:753	the α-glucan starch/pullulan	726:753	the α-glucan starch/pullulan compared to glucose	726:773	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	8	49	theme	control	1357:1363	arg1	strategies					1365:1374	future control strategies	1350:1374	future control strategies including the development of anti-infective strategies	1350:1429	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	8	49	theme	control	1357:1363	arg1	development					1390:1400	the development	1386:1400	the development of anti-infective strategies	1386:1429	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	7	50	from	effect	1122:1127	arg1	expression					1164:1173	expression	1164:1173	expression of virulence genes in the mucosa, organs and blood	1164:1224	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	7	51	theme	in	1024:1025	arg1	studies					1061:1067	in vitro and in vivo gene expression studies	1024:1067	in vitro and in vivo gene expression studies	1024:1067	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	5	52	theme	higher	681:686	arg1	levels					688:693	higher levels	681:693	higher levels	681:693	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	7	53	from	expression	1164:1173	arg1	mucosa					1201:1206	the mucosa	1197:1206	the mucosa	1197:1206	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	7	53	from	expression	1164:1173	arg1	blood					1220:1224	blood	1220:1224	blood	1220:1224	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	7	53	from	expression	1164:1173	arg1	organs					1209:1214	organs	1209:1214	organs	1209:1214	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	8	54	theme	future	1350:1355	arg1	strategies					1365:1374	future control strategies	1350:1374	future control strategies including the development of anti-infective strategies	1350:1429	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	8	54	theme	future	1350:1355	arg1	development					1390:1400	the development	1386:1400	the development of anti-infective strategies	1386:1429	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	7	55	theme	biological	1085:1094	arg1	model					1096:1100	a biological model	1083:1100	a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood	1083:1224	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	8	56	dep	S.	1272:1273	arg1	suis					1275:1278	suis	1275:1278	suis	1275:1278	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	1	57	theme	young	137:141	arg1	pigs					143:146	young pigs	137:146	young pigs causing worldwide economic problems for the pig industry	137:203	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	8	58	theme	strategies	1365:1374	arg1	design					1340:1345	the design	1336:1345	the design of future control strategies including the development of anti-infective strategies	1336:1429	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	1	59	theme	pigs	143:146	arg1	pathogen					125:132	a major bacterial pathogen	107:132	a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry	107:203	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	1	59	theme	pigs	143:146	arg1	suis					99:102	Streptococcus suis	85:102	Streptococcus suis	85:102	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	5	60	dep	S.	581:582	arg1	suis					584:587	suis	584:587	suis	584:587	Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source.
24642967	7	61	theme	virulence	1178:1186	arg1	genes					1188:1192	virulence genes	1178:1192	virulence genes	1178:1192	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	8	62	theme	feed	1452:1455	arg1	composition					1457:1467	animal feed composition	1445:1467	animal feed composition	1445:1467	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	2	63	theme	humans	246:251	arg1	pathogen					234:241	an emerging pathogen	222:241	an emerging pathogen of humans	222:251	S. suis is also an emerging pathogen of humans.
24642967	2	63	theme	humans	246:251	arg1	suis					209:212	S. suis	206:212	S. suis	206:212	S. suis is also an emerging pathogen of humans.
24642967	7	64	theme	genes	1188:1192	arg1	expression					1164:1173	expression	1164:1173	expression of virulence genes in the mucosa, organs and blood	1164:1224	Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood.
24642967	3	65	theme	high	322:325	arg1	factor					332:337	a high risk factor	320:337	a high risk factor for invasive disease	320:358	Colonization of porcine oropharynx by S. suis is considered to be a high risk factor for invasive disease.
24642967	3	65	theme	high	322:325	arg1	Colonization					254:265	Colonization	254:265	Colonization of porcine oropharynx by S. suis	254:298	Colonization of porcine oropharynx by S. suis is considered to be a high risk factor for invasive disease.
24642967	1	66	theme	worldwide	156:164	arg1	problems					175:182	worldwide economic problems	156:182	worldwide economic problems for the pig industry	156:203	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry.
24642967	8	67	contain	has	1305:1307	arg2	implications					1319:1330	important implications	1309:1330	important implications for the design of future control strategies including the development of anti-infective strategies	1309:1429	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
24642967	8	67	contain	has	1305:1307	arg1	research					1232:1239	This research	1227:1239	This research	1227:1239	This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
26178692	6	0	with	materials	973:981	arg1	structures					1001:1010	hierarchical structures	988:1010	hierarchical structures toward extensive applications in science and technology	988:1066	This work sets up an efficient strategy for the fabrication of stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology.
26178692	6	1	theme	materials	973:981	arg1	fabrication					930:940	the fabrication	926:940	the fabrication of stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology	926:1066	This work sets up an efficient strategy for the fabrication of stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology.
26178692	5	2	theme	PNIPAM	844:849	arg1	change					834:839	the volume change	823:839	the volume change of PNIPAM	823:849	This unique phenomenon is caused by the refractive index change due to the volume change of PNIPAM during the temperature rising.
26178692	4	3	with	red-shift	617:625	arg1	increase					632:639	increase	632:639	increase in temperature-an opposite phenomenon	632:677	The corresponding reflection peaks red-shift with increase in temperature-an opposite phenomenon to previous studies, demonstrating a thermoresponsive photonic property.
26178692	1	4	theme	chemical	286:293	arg1	bonding					295:301	a chemical bonding	284:301	a chemical bonding	284:301	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	3	5	theme	NIPAM	415:419	arg1	monomers					421:428	NIPAM monomers	415:428	NIPAM monomers	415:428	Through reacting with both NIPAM monomers and the amino groups of chitosan in wing scales, glutaraldehyde workes as a bridge by creating chemical bonding between the biotemplate and the PNIPAM.
26178692	3	5	theme	NIPAM	415:419	arg1	chitosan					454:461	chitosan	454:461	chitosan	454:461	Through reacting with both NIPAM monomers and the amino groups of chitosan in wing scales, glutaraldehyde workes as a bridge by creating chemical bonding between the biotemplate and the PNIPAM.
26178692	3	6	from	monomers	421:428	arg1	scales					471:476	wing scales	466:476	wing scales	466:476	Through reacting with both NIPAM monomers and the amino groups of chitosan in wing scales, glutaraldehyde workes as a bridge by creating chemical bonding between the biotemplate and the PNIPAM.
26178692	1	7	theme	Thermoresponsive	105:120	arg1	materials					131:139	Thermoresponsive photonic materials	105:139	Thermoresponsive photonic materials having hierarchical structures	105:170	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	4	8	from	increase	632:639	arg1	phenomenon					668:677	temperature-an opposite phenomenon	644:677	temperature-an opposite phenomenon	644:677	The corresponding reflection peaks red-shift with increase in temperature-an opposite phenomenon to previous studies, demonstrating a thermoresponsive photonic property.
26178692	3	9	theme	chemical	525:532	arg1	bonding					534:540	chemical bonding	525:540	chemical bonding between the biotemplate and the PNIPAM	525:579	Through reacting with both NIPAM monomers and the amino groups of chitosan in wing scales, glutaraldehyde workes as a bridge by creating chemical bonding between the biotemplate and the PNIPAM.
26178692	1	10	theme	butterfly	218:226	arg1	wings					228:232	Morpho butterfly wings	211:232	Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM)	211:274	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	4	11	theme	photonic	733:740	arg1	property					742:749	a thermoresponsive photonic property	714:749	a thermoresponsive photonic property	714:749	The corresponding reflection peaks red-shift with increase in temperature-an opposite phenomenon to previous studies, demonstrating a thermoresponsive photonic property.
26178692	6	12	theme	stimuli-responsive	945:962	arg1	materials					973:981	stimuli-responsive photonic materials	945:981	stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology	945:1066	This work sets up an efficient strategy for the fabrication of stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology.
26178692	0	13	theme	Hierarchical	52:63	arg1	Structures					65:74	Hierarchical Structures	52:74	Hierarchical Structures	52:74	Bioinspired Thermoresponsive Photonic Polymers with Hierarchical Structures and Their Unique Properties.
26178692	1	14	theme	Morpho	211:216	arg1	wings					228:232	Morpho butterfly wings	211:232	Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM)	211:274	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	0	15	theme	Thermoresponsive	12:27	arg1	Polymers					38:45	Thermoresponsive Photonic Polymers	12:45	Thermoresponsive Photonic Polymers	12:45	Bioinspired Thermoresponsive Photonic Polymers with Hierarchical Structures and Their Unique Properties.
26178692	6	16	theme	photonic	964:971	arg1	materials					973:981	stimuli-responsive photonic materials	945:981	stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology	945:1066	This work sets up an efficient strategy for the fabrication of stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology.
26178692	2	17	theme	temperature-induced	350:368	arg1	tunability					376:385	temperature-induced color tunability	350:385	temperature-induced color tunability	350:385	These materials show temperature-induced color tunability.
26178692	5	18	theme	unique	757:762	arg1	phenomenon					764:773	This unique phenomenon	752:773	This unique phenomenon	752:773	This unique phenomenon is caused by the refractive index change due to the volume change of PNIPAM during the temperature rising.
26178692	3	19	theme	wing	466:469	arg1	scales					471:476	wing scales	466:476	wing scales	466:476	Through reacting with both NIPAM monomers and the amino groups of chitosan in wing scales, glutaraldehyde workes as a bridge by creating chemical bonding between the biotemplate and the PNIPAM.
26178692	3	20	theme	amino	438:442	arg1	chitosan					454:461	chitosan	454:461	chitosan	454:461	Through reacting with both NIPAM monomers and the amino groups of chitosan in wing scales, glutaraldehyde workes as a bridge by creating chemical bonding between the biotemplate and the PNIPAM.
26178692	3	20	theme	amino	438:442	arg1	groups					444:449	the amino groups	434:449	the amino groups of chitosan in wing scales	434:476	Through reacting with both NIPAM monomers and the amino groups of chitosan in wing scales, glutaraldehyde workes as a bridge by creating chemical bonding between the biotemplate and the PNIPAM.
26178692	1	21	theme	photonic	122:129	arg1	materials					131:139	Thermoresponsive photonic materials	105:139	Thermoresponsive photonic materials having hierarchical structures	105:170	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	1	22	theme	wings	228:232	arg1	template					199:206	a template	197:206	a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM)	197:274	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	1	23	theme	polymerization	307:320	arg1	route					322:326	polymerization route	307:326	polymerization route	307:326	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	0	24	theme	Photonic	29:36	arg1	Polymers					38:45	Thermoresponsive Photonic Polymers	12:45	Thermoresponsive Photonic Polymers	12:45	Bioinspired Thermoresponsive Photonic Polymers with Hierarchical Structures and Their Unique Properties.
26178692	3	25	theme	chitosan	454:461	arg1	monomers					421:428	NIPAM monomers	415:428	NIPAM monomers	415:428	Through reacting with both NIPAM monomers and the amino groups of chitosan in wing scales, glutaraldehyde workes as a bridge by creating chemical bonding between the biotemplate and the PNIPAM.
26178692	3	25	theme	chitosan	454:461	arg1	chitosan					454:461	chitosan	454:461	chitosan	454:461	Through reacting with both NIPAM monomers and the amino groups of chitosan in wing scales, glutaraldehyde workes as a bridge by creating chemical bonding between the biotemplate and the PNIPAM.
26178692	3	25	theme	chitosan	454:461	arg1	groups					444:449	the amino groups	434:449	the amino groups of chitosan in wing scales	434:476	Through reacting with both NIPAM monomers and the amino groups of chitosan in wing scales, glutaraldehyde workes as a bridge by creating chemical bonding between the biotemplate and the PNIPAM.
26178692	1	26	contain	having	141:146	arg1	materials					131:139	Thermoresponsive photonic materials	105:139	Thermoresponsive photonic materials having hierarchical structures	105:170	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	1	26	contain	having	141:146	arg2	structures					161:170	hierarchical structures	148:170	hierarchical structures	148:170	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	5	27	theme	refractive	792:801	arg1	index					803:807	the refractive index	788:807	the refractive index change due to the volume change of PNIPAM during the temperature rising	788:879	This unique phenomenon is caused by the refractive index change due to the volume change of PNIPAM during the temperature rising.
26178692	6	28	from	applications	1029:1040	arg1	technology					1057:1066	technology	1057:1066	technology	1057:1066	This work sets up an efficient strategy for the fabrication of stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology.
26178692	6	28	from	applications	1029:1040	arg1	science					1045:1051	science	1045:1051	science	1045:1051	This work sets up an efficient strategy for the fabrication of stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology.
26178692	4	29	theme	corresponding	586:598	arg1	reflection					600:609	The corresponding reflection	582:609	The corresponding reflection	582:609	The corresponding reflection peaks red-shift with increase in temperature-an opposite phenomenon to previous studies, demonstrating a thermoresponsive photonic property.
26178692	1	30	theme	hierarchical	148:159	arg1	structures					161:170	hierarchical structures	148:170	hierarchical structures	148:170	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	5	31	theme	temperature	862:872	arg1	rising					874:879	the temperature rising	858:879	the temperature rising	858:879	This unique phenomenon is caused by the refractive index change due to the volume change of PNIPAM during the temperature rising.
26178692	1	32	with	wings	228:232	arg1	PNIPAM					268:273	PNIPAM	268:273	PNIPAM	268:273	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	1	32	with	wings	228:232	arg1	poly					239:242	poly	239:242	poly(N-isopropylacrylamide) (PNIPAM)	239:274	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	1	32	with	wings	228:232	arg1	N-isopropylacrylamide					244:264	N-isopropylacrylamide	244:264	N-isopropylacrylamide	244:264	Thermoresponsive photonic materials having hierarchical structures are created by combining a template of Morpho butterfly wings with poly(N-isopropylacrylamide) (PNIPAM) through a chemical bonding and polymerization route.
26178692	6	33	theme	efficient	903:911	arg1	strategy					913:920	an efficient strategy	900:920	an efficient strategy for the fabrication of stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology	900:1066	This work sets up an efficient strategy for the fabrication of stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology.
26178692	6	34	theme	extensive	1019:1027	arg1	applications					1029:1040	extensive applications	1019:1040	extensive applications in science and technology	1019:1066	This work sets up an efficient strategy for the fabrication of stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology.
26178692	5	35	dep	index	803:807	arg1	change					809:814	change	809:814	change due to the volume change of PNIPAM during the temperature rising	809:879	This unique phenomenon is caused by the refractive index change due to the volume change of PNIPAM during the temperature rising.
26178692	5	36	theme	volume	827:832	arg1	change					834:839	the volume change	823:839	the volume change of PNIPAM	823:849	This unique phenomenon is caused by the refractive index change due to the volume change of PNIPAM during the temperature rising.
26178692	6	37	theme	hierarchical	988:999	arg1	structures					1001:1010	hierarchical structures	988:1010	hierarchical structures toward extensive applications in science and technology	988:1066	This work sets up an efficient strategy for the fabrication of stimuli-responsive photonic materials with hierarchical structures toward extensive applications in science and technology.
26178692	2	38	theme	color	370:374	arg1	tunability					376:385	temperature-induced color tunability	350:385	temperature-induced color tunability	350:385	These materials show temperature-induced color tunability.
26178692	4	39	theme	opposite	659:666	arg1	phenomenon					668:677	temperature-an opposite phenomenon	644:677	temperature-an opposite phenomenon	644:677	The corresponding reflection peaks red-shift with increase in temperature-an opposite phenomenon to previous studies, demonstrating a thermoresponsive photonic property.
26178692	0	40	theme	Unique	86:91	arg1	Properties					93:102	Their Unique Properties	80:102	Their Unique Properties	80:102	Bioinspired Thermoresponsive Photonic Polymers with Hierarchical Structures and Their Unique Properties.
26178692	4	41	theme	thermoresponsive	716:731	arg1	property					742:749	a thermoresponsive photonic property	714:749	a thermoresponsive photonic property	714:749	The corresponding reflection peaks red-shift with increase in temperature-an opposite phenomenon to previous studies, demonstrating a thermoresponsive photonic property.
26178692	4	42	theme	previous	682:689	arg1	studies					691:697	previous studies	682:697	previous studies	682:697	The corresponding reflection peaks red-shift with increase in temperature-an opposite phenomenon to previous studies, demonstrating a thermoresponsive photonic property.
26178692	4	43	theme	temperature-an	644:657	arg1	phenomenon					668:677	temperature-an opposite phenomenon	644:677	temperature-an opposite phenomenon	644:677	The corresponding reflection peaks red-shift with increase in temperature-an opposite phenomenon to previous studies, demonstrating a thermoresponsive photonic property.
26178692	3	44	from	groups	444:449	arg1	scales					471:476	wing scales	466:476	wing scales	466:476	Through reacting with both NIPAM monomers and the amino groups of chitosan in wing scales, glutaraldehyde workes as a bridge by creating chemical bonding between the biotemplate and the PNIPAM.
27987426	6	0	theme	menisci	981:987	arg1	sites					920:924	all the measurement sites	900:924	all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26)	900:994	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	6	0	theme	menisci	981:987	arg1	menisci					981:987	lateral and medial menisci	962:987	lateral and medial menisci (n=26)	962:994	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	8	1	theme	entire	1545:1550	arg1	depth					1559:1563	the entire tissue depth	1541:1563	the entire tissue depth	1541:1563	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	11	2	theme	higher	2330:2335	arg1	content					2346:2352	higher collagen content	2330:2352	higher collagen content at all tissue depths and superficial collagen fibril orientation	2330:2417	These nonlinear biomechanical properties are suggested to be further amplified by higher collagen content at all tissue depths and superficial collagen fibril orientation.
27987426	5	3	theme	orientation	732:742	arg1	contents					698:705	Proteoglycan (PG) and collagen contents	667:705	Proteoglycan (PG) and collagen contents	667:705	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	5	3	theme	orientation	732:742	arg1	angle					744:748	the collagen orientation angle	719:748	the collagen orientation angle	719:748	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	5	3	theme	orientation	732:742	arg1	function					772:779	a function	770:779	a function of tissue depth using microscopic and spectroscopic methods	770:839	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	2	4	from	depths	198:203	arg1	constituents					172:183	meniscus constituents	163:183	meniscus constituents at different depths and locations	163:217	However, it is not known which meniscus constituents at different depths and locations contribute to biomechanical properties obtained from indentation testing.
27987426	10	5	theme	fibril	2232:2237	arg1	tension					2239:2245	increased collagen fibril tension	2213:2245	increased collagen fibril tension	2213:2245	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	3	6	with	relationships	416:428	arg1	properties					470:479	tissue site-specific biomechanical properties	435:479	tissue site-specific biomechanical properties	435:479	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	2	7	from	locations	209:217	arg1	constituents					172:183	meniscus constituents	163:183	meniscus constituents at different depths and locations	163:217	However, it is not known which meniscus constituents at different depths and locations contribute to biomechanical properties obtained from indentation testing.
27987426	9	8	theme	PG	1883:1884	arg1	content					1886:1892	the PG content	1879:1892	the PG content	1879:1892	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	8	9	dep	lower	1570:1574	arg1	p<0.05					1577:1582	p<0.05	1577:1582	p<0.05	1577:1582	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	9	10	dep	higher	1791:1796	arg1	p<0.05					1799:1804	p<0.05	1799:1804	p<0.05	1799:1804	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	6	11	theme	collagen	1012:1019	arg1	angle					1033:1037	collagen orientation angle	1012:1037	collagen orientation angle	1012:1037	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	9	12	theme	other	1683:1687	arg1	hand					1689:1692	the other hand	1679:1692	the other hand	1679:1692	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	10	13	from	meniscus	2157:2164	arg1	swelling					2134:2141	tissue osmotic swelling	2119:2141	tissue osmotic swelling from the deep meniscus caused by the increased PG content	2119:2199	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	11	14	theme	collagen	2391:2398	arg1	orientation					2407:2417	superficial collagen fibril orientation	2379:2417	superficial collagen fibril orientation	2379:2417	These nonlinear biomechanical properties are suggested to be further amplified by higher collagen content at all tissue depths and superficial collagen fibril orientation.
27987426	11	15	theme	nonlinear	2254:2262	arg1	properties					2278:2287	These nonlinear biomechanical properties	2248:2287	These nonlinear biomechanical properties	2248:2287	These nonlinear biomechanical properties are suggested to be further amplified by higher collagen content at all tissue depths and superficial collagen fibril orientation.
27987426	5	16	theme	depth	791:795	arg1	angle					744:748	the collagen orientation angle	719:748	the collagen orientation angle	719:748	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	5	16	theme	depth	791:795	arg1	contents					698:705	Proteoglycan (PG) and collagen contents	667:705	Proteoglycan (PG) and collagen contents	667:705	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	5	16	theme	depth	791:795	arg1	function					772:779	a function	770:779	a function of tissue depth using microscopic and spectroscopic methods	770:839	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	2	17	theme	biomechanical	233:245	arg1	properties					247:256	biomechanical properties	233:256	biomechanical properties obtained from indentation testing	233:290	However, it is not known which meniscus constituents at different depths and locations contribute to biomechanical properties obtained from indentation testing.
27987426	9	18	theme	certain	1698:1704	arg1	comparisons					1706:1716	certain comparisons	1698:1716	certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle	1698:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	18	theme	certain	1698:1704	arg1	content					1816:1822	significantly higher (p<0.05) collagen content	1777:1822	significantly higher (p<0.05) collagen content	1777:1822	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	18	theme	certain	1698:1704	arg1	angle					1846:1850	lower orientation angle	1828:1850	lower orientation angle	1828:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	7	19	theme	medial	1416:1421	arg1	meniscus					1423:1430	the medial meniscus	1412:1430	the medial meniscus	1412:1430	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	9	20	theme	increased	1916:1924	arg1	modulus					1935:1941	increased meniscus modulus	1916:1941	increased meniscus modulus	1916:1941	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	3	21	from	structure	340:348	arg1	manner					399:404	a site- and depth-dependent manner	371:404	a site- and depth-dependent manner	371:404	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	10	22	theme	osmotic	2126:2132	arg1	swelling					2134:2141	tissue osmotic swelling	2119:2141	tissue osmotic swelling from the deep meniscus caused by the increased PG content	2119:2199	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	11	23	theme	fibril	2400:2405	arg1	orientation					2407:2417	superficial collagen fibril orientation	2379:2417	superficial collagen fibril orientation	2379:2417	These nonlinear biomechanical properties are suggested to be further amplified by higher collagen content at all tissue depths and superficial collagen fibril orientation.
27987426	7	24	located	observed	1379:1386	arg2	p<0.05					1200:1205	p<0.05	1200:1205	p<0.05	1200:1205	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	24	located	observed	1379:1386	arg2	values					1192:1197	The highest values	1180:1197	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability)	1180:1372	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	24	located	observed	1379:1386	arg1	horn					1404:1407	the anterior horn	1391:1407	the anterior horn of the medial meniscus	1391:1430	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	2	25	theme	indentation	272:282	arg1	testing					284:290	indentation testing	272:290	indentation testing	272:290	However, it is not known which meniscus constituents at different depths and locations contribute to biomechanical properties obtained from indentation testing.
27987426	6	26	theme	tissue	1066:1071	arg1	depth					1073:1077	tissue depth	1066:1077	tissue depth	1066:1077	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	3	27	theme	human	353:357	arg1	meniscus					359:366	human meniscus	353:366	human meniscus in a site- and depth-dependent manner	353:404	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	8	28	theme	higher	1505:1510	arg1	content					1530:1536	higher (p<0.05) collagen content	1505:1536	higher (p<0.05) collagen content in the entire tissue depth	1505:1563	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	7	29	theme	anterior	1395:1402	arg1	horn					1404:1407	the anterior horn	1391:1407	the anterior horn of the medial meniscus	1391:1430	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	9	30	theme	nonlinear	1950:1958	arg1	permeability					1960:1971	nonlinear permeability	1950:1971	nonlinear permeability	1950:1971	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	3	31	from	relationships	416:428	arg1	manner					399:404	a site- and depth-dependent manner	371:404	a site- and depth-dependent manner	371:404	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	9	32	from	comparisons	1706:1716	arg1	consistent					1900:1909	consistent	1900:1909	consistent	1900:1909	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	12	33	theme	structure-function	2435:2452	arg1	relationships					2454:2466	these structure-function relationships	2429:2466	these structure-function relationships	2429:2466	However, these structure-function relationships are suggested to be highly site-specific.
27987426	10	34	theme	deep	2152:2155	arg1	meniscus					2157:2164	the deep meniscus	2148:2164	the deep meniscus	2148:2164	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	7	35	theme	instantaneous	1247:1259	arg1	moduli					1261:1266	instantaneous moduli	1247:1266	instantaneous moduli	1247:1266	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	3	36	theme	site-	373:377	arg1	manner					399:404	a site- and depth-dependent manner	371:404	a site- and depth-dependent manner	371:404	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	8	37	theme	collagen	1521:1528	arg1	content					1530:1536	higher (p<0.05) collagen content	1505:1536	higher (p<0.05) collagen content in the entire tissue depth	1505:1563	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	10	38	theme	PG	2190:2191	arg1	content					2193:2199	the increased PG content	2176:2199	the increased PG content	2176:2199	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	10	39	theme	biomechanical	2009:2021	arg1	properties					2023:2032	nonlinear biomechanical properties	1999:2032	nonlinear biomechanical properties	1999:2032	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	5	40	theme	microscopic	803:813	arg1	methods					833:839	microscopic and spectroscopic methods	803:839	microscopic and spectroscopic methods	803:839	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	1	41	theme	human	80:84	arg1	meniscus					86:93	human meniscus	80:93	human meniscus	80:93	Biomechanical properties of human meniscus have been shown to be site-specific.
27987426	5	42	theme	spectroscopic	819:831	arg1	methods					833:839	microscopic and spectroscopic methods	803:839	microscopic and spectroscopic methods	803:839	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	5	43	theme	Proteoglycan	667:678	arg1	angle					744:748	the collagen orientation angle	719:748	the collagen orientation angle	719:748	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	5	43	theme	Proteoglycan	667:678	arg1	contents					698:705	Proteoglycan (PG) and collagen contents	667:705	Proteoglycan (PG) and collagen contents	667:705	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	5	43	theme	Proteoglycan	667:678	arg1	function					772:779	a function	770:779	a function of tissue depth using microscopic and spectroscopic methods	770:839	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	6	44	theme	initial	1113:1119	arg1	increase					1127:1134	an initial sharp increase	1110:1134	an initial sharp increase followed by a decrease across tissue depth	1110:1177	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	9	45	theme	meniscus	1767:1774	arg1	meniscus					1767:1774	the medial meniscus	1756:1774	the medial meniscus	1756:1774	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	45	theme	meniscus	1767:1774	arg1	sites					1747:1751	anterior vs. middle sites	1727:1751	anterior vs. middle sites of the medial meniscus	1727:1774	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	4	46	theme	experimental	536:547	arg1	stress-relaxation					549:565	experimental stress-relaxation and sinusoidal indentation measurements	536:605	stress-relaxation	549:565	Elastic and poroelastic properties were analyzed from experimental stress-relaxation and sinusoidal indentation measurements with fibril reinforced poroelastic finite element modeling.
27987426	11	47	theme	superficial	2379:2389	arg1	orientation					2407:2417	superficial collagen fibril orientation	2379:2417	superficial collagen fibril orientation	2379:2417	These nonlinear biomechanical properties are suggested to be further amplified by higher collagen content at all tissue depths and superficial collagen fibril orientation.
27987426	3	48	theme	biomechanical	456:468	arg1	properties					470:479	tissue site-specific biomechanical properties	435:479	tissue site-specific biomechanical properties	435:479	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	0	49	theme	Structure-function	0:17	arg1	relationships					19:31	Structure-function relationships	0:31	Structure-function relationships of human meniscus	0:49	Structure-function relationships of human meniscus.
27987426	3	50	theme	depth-dependent	383:397	arg1	manner					399:404	a site- and depth-dependent manner	371:404	a site- and depth-dependent manner	371:404	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	4	51	theme	finite	642:647	arg1	element					649:655	fibril reinforced poroelastic finite element	612:655	fibril reinforced poroelastic finite element modeling	612:664	Elastic and poroelastic properties were analyzed from experimental stress-relaxation and sinusoidal indentation measurements with fibril reinforced poroelastic finite element modeling.
27987426	7	52	dep	parameters	1304:1313	arg1	permeability					1360:1371	permeability	1360:1371	permeability	1360:1371	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	52	dep	parameters	1304:1313	arg1	modulus					1348:1354	strain-dependent fibril network modulus	1316:1354	strain-dependent fibril network modulus	1316:1354	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	52	dep	parameters	1304:1313	arg1	parameters					1304:1313	strain-dependent biomechanical parameters	1273:1313	strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability)	1273:1372	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	6	53	theme	collagen	1089:1096	arg1	content					1098:1104	the collagen content	1085:1104	the collagen content	1085:1104	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	3	54	theme	tissue	435:440	arg1	properties					470:479	tissue site-specific biomechanical properties	435:479	tissue site-specific biomechanical properties	435:479	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	6	55	dep	sites	920:924	arg1	posterior					948:956	posterior	948:956	posterior	948:956	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	6	55	dep	sites	920:924	arg1	middle					937:942	middle	937:942	middle	937:942	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	6	55	dep	sites	920:924	arg1	anterior					927:934	anterior	927:934	anterior	927:934	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	9	56	with	consistent	1900:1909	arg1	modulus					1935:1941	increased meniscus modulus	1916:1941	increased meniscus modulus	1916:1941	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	56	with	consistent	1900:1909	arg1	permeability					1960:1971	nonlinear permeability	1950:1971	nonlinear permeability	1950:1971	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	11	57	from	orientation	2407:2417	arg1	content					2346:2352	higher collagen content	2330:2352	higher collagen content at all tissue depths and superficial collagen fibril orientation	2330:2417	These nonlinear biomechanical properties are suggested to be further amplified by higher collagen content at all tissue depths and superficial collagen fibril orientation.
27987426	0	58	theme	meniscus	42:49	arg1	relationships					19:31	Structure-function relationships	0:31	Structure-function relationships of human meniscus	0:49	Structure-function relationships of human meniscus.
27987426	3	59	theme	relationships	416:428	arg1	structure					340:348	structure	340:348	structure	340:348	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	3	59	theme	relationships	416:428	arg1	composition					324:334	composition	324:334	composition	324:334	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	9	60	theme	anterior	1727:1734	arg1	meniscus					1767:1774	the medial meniscus	1756:1774	the medial meniscus	1756:1774	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	60	theme	anterior	1727:1734	arg1	sites					1747:1751	anterior vs. middle sites	1727:1751	anterior vs. middle sites of the medial meniscus	1727:1774	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	8	61	theme	higher	1456:1461	arg1	content					1475:1481	also higher (p<0.05) PG content	1451:1481	also higher (p<0.05) PG content in the deep meniscus	1451:1502	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	5	62	theme	biomechanical	870:882	arg1	properties					884:893	biomechanical properties	870:893	biomechanical properties	870:893	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	4	63	theme	sinusoidal	571:580	arg1	measurements					594:605	experimental stress-relaxation and sinusoidal indentation measurements	536:605	measurements	594:605	Elastic and poroelastic properties were analyzed from experimental stress-relaxation and sinusoidal indentation measurements with fibril reinforced poroelastic finite element modeling.
27987426	6	64	theme	tissue	1166:1171	arg1	depth					1173:1177	tissue depth	1166:1177	tissue depth	1166:1177	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	4	65	theme	reinforced	619:628	arg1	element					649:655	fibril reinforced poroelastic finite element	612:655	fibril reinforced poroelastic finite element modeling	612:664	Elastic and poroelastic properties were analyzed from experimental stress-relaxation and sinusoidal indentation measurements with fibril reinforced poroelastic finite element modeling.
27987426	6	66	theme	measurement	908:918	arg1	sites					920:924	all the measurement sites	900:924	all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26)	900:994	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	6	66	theme	measurement	908:918	arg1	menisci					981:987	lateral and medial menisci	962:987	lateral and medial menisci (n=26)	962:994	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	7	67	theme	strain-dependent	1316:1331	arg1	modulus					1348:1354	strain-dependent fibril network modulus	1316:1354	strain-dependent fibril network modulus	1316:1354	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	67	theme	strain-dependent	1316:1331	arg1	parameters					1304:1313	strain-dependent biomechanical parameters	1273:1313	strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability)	1273:1372	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	9	68	theme	lower	1828:1832	arg1	comparisons					1706:1716	certain comparisons	1698:1716	certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle	1698:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	68	theme	lower	1828:1832	arg1	angle					1846:1850	lower orientation angle	1828:1850	lower orientation angle	1828:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	8	69	theme	orientation	1594:1604	arg1	angle					1606:1610	lower (p<0.05) collagen orientation angle	1570:1610	lower (p<0.05) collagen orientation angle at the superficial tissue	1570:1636	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	2	70	theme	different	188:196	arg1	depths					198:203	different depths	188:203	different depths	188:203	However, it is not known which meniscus constituents at different depths and locations contribute to biomechanical properties obtained from indentation testing.
27987426	8	71	theme	PG	1472:1473	arg1	content					1475:1481	also higher (p<0.05) PG content	1451:1481	also higher (p<0.05) PG content in the deep meniscus	1451:1502	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	8	72	theme	many	1654:1657	arg1	locations					1665:1673	many other locations	1654:1673	many other locations	1654:1673	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	3	73	from	composition	324:334	arg1	manner					399:404	a site- and depth-dependent manner	371:404	a site- and depth-dependent manner	371:404	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	7	74	theme	parameters	1304:1313	arg1	p<0.05					1200:1205	p<0.05	1200:1205	p<0.05	1200:1205	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	74	theme	parameters	1304:1313	arg1	values					1192:1197	The highest values	1180:1197	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability)	1180:1372	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	5	75	theme	collagen	723:730	arg1	contents					698:705	Proteoglycan (PG) and collagen contents	667:705	Proteoglycan (PG) and collagen contents	667:705	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	5	75	theme	collagen	723:730	arg1	angle					744:748	the collagen orientation angle	719:748	the collagen orientation angle	719:748	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	5	75	theme	collagen	723:730	arg1	function					772:779	a function	770:779	a function of tissue depth using microscopic and spectroscopic methods	770:839	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	6	76	theme	medial	974:979	arg1	n=26					990:993	n=26	990:993	n=26	990:993	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	6	76	theme	medial	974:979	arg1	menisci					981:987	lateral and medial menisci	962:987	lateral and medial menisci (n=26)	962:994	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	7	77	theme	strain-dependent	1273:1288	arg1	permeability					1360:1371	permeability	1360:1371	permeability	1360:1371	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	77	theme	strain-dependent	1273:1288	arg1	modulus					1348:1354	strain-dependent fibril network modulus	1316:1354	strain-dependent fibril network modulus	1316:1354	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	77	theme	strain-dependent	1273:1288	arg1	parameters					1304:1313	strain-dependent biomechanical parameters	1273:1313	strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability)	1273:1372	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	10	78	theme	collagen	2061:2068	arg1	network					2070:2076	the collagen network	2057:2076	the collagen network	2057:2076	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	8	79	theme	superficial	1619:1629	arg1	tissue					1631:1636	the superficial tissue	1615:1636	the superficial tissue	1615:1636	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	6	80	theme	lateral	962:968	arg1	n=26					990:993	n=26	990:993	n=26	990:993	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	6	80	theme	lateral	962:968	arg1	menisci					981:987	lateral and medial menisci	962:987	lateral and medial menisci (n=26)	962:994	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	10	81	theme	collagen	2223:2230	arg1	tension					2239:2245	increased collagen fibril tension	2213:2245	increased collagen fibril tension	2213:2245	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	3	82	dep	composition	324:334	arg1	the					320:322	the	320:322	the	320:322	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	9	83	theme	collagen	1807:1814	arg1	comparisons					1706:1716	certain comparisons	1698:1716	certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle	1698:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	83	theme	collagen	1807:1814	arg1	content					1816:1822	significantly higher (p<0.05) collagen content	1777:1822	significantly higher (p<0.05) collagen content	1777:1822	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	4	84	theme	poroelastic	630:640	arg1	element					649:655	fibril reinforced poroelastic finite element	612:655	fibril reinforced poroelastic finite element modeling	612:664	Elastic and poroelastic properties were analyzed from experimental stress-relaxation and sinusoidal indentation measurements with fibril reinforced poroelastic finite element modeling.
27987426	8	85	from	content	1475:1481	arg1	depth					1559:1563	the entire tissue depth	1541:1563	the entire tissue depth	1541:1563	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	8	85	from	content	1475:1481	arg1	meniscus					1495:1502	the deep meniscus	1486:1502	the deep meniscus	1486:1502	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	6	86	theme	orientation	1021:1031	arg1	angle					1033:1037	collagen orientation angle	1012:1037	collagen orientation angle	1012:1037	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	10	87	theme	tissue	2119:2124	arg1	swelling					2134:2141	tissue osmotic swelling	2119:2141	tissue osmotic swelling from the deep meniscus caused by the increased PG content	2119:2199	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	9	88	from	difference	1865:1874	arg1	content					1886:1892	the PG content	1879:1892	the PG content	1879:1892	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	4	89	theme	poroelastic	494:504	arg1	properties					506:515	Elastic and poroelastic properties	482:515	Elastic and poroelastic properties	482:515	Elastic and poroelastic properties were analyzed from experimental stress-relaxation and sinusoidal indentation measurements with fibril reinforced poroelastic finite element modeling.
27987426	11	90	theme	biomechanical	2264:2276	arg1	properties					2278:2287	These nonlinear biomechanical properties	2248:2287	These nonlinear biomechanical properties	2248:2287	These nonlinear biomechanical properties are suggested to be further amplified by higher collagen content at all tissue depths and superficial collagen fibril orientation.
27987426	7	91	theme	network	1340:1346	arg1	modulus					1348:1354	strain-dependent fibril network modulus	1316:1354	strain-dependent fibril network modulus	1316:1354	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	91	theme	network	1340:1346	arg1	parameters					1304:1313	strain-dependent biomechanical parameters	1273:1313	strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability)	1273:1372	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	5	92	theme	tissue	784:789	arg1	depth					791:795	tissue depth	784:795	tissue depth using microscopic and spectroscopic methods	784:839	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	6	93	theme	PG	997:998	arg1	content					1000:1006	PG content	997:1006	PG content	997:1006	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	8	94	theme	deep	1490:1493	arg1	meniscus					1495:1502	the deep meniscus	1486:1502	the deep meniscus	1486:1502	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	9	95	from	consistent	1900:1909	arg1	comparisons					1706:1716	certain comparisons	1698:1716	certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle	1698:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	95	from	consistent	1900:1909	arg1	content					1816:1822	significantly higher (p<0.05) collagen content	1777:1822	significantly higher (p<0.05) collagen content	1777:1822	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	95	from	consistent	1900:1909	arg1	angle					1846:1850	lower orientation angle	1828:1850	lower orientation angle	1828:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	4	96	theme	indentation	582:592	arg1	measurements					594:605	experimental stress-relaxation and sinusoidal indentation measurements	536:605	measurements	594:605	Elastic and poroelastic properties were analyzed from experimental stress-relaxation and sinusoidal indentation measurements with fibril reinforced poroelastic finite element modeling.
27987426	7	97	theme	meniscus	1423:1430	arg1	horn					1404:1407	the anterior horn	1391:1407	the anterior horn of the medial meniscus	1391:1430	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	9	98	theme	meniscus	1926:1933	arg1	modulus					1935:1941	increased meniscus modulus	1916:1941	increased meniscus modulus	1916:1941	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	8	99	from	tissue	1631:1636	arg1	content					1475:1481	also higher (p<0.05) PG content	1451:1481	also higher (p<0.05) PG content in the deep meniscus	1451:1502	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	8	99	from	tissue	1631:1636	arg1	content					1530:1536	higher (p<0.05) collagen content	1505:1536	higher (p<0.05) collagen content in the entire tissue depth	1505:1563	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	8	99	from	tissue	1631:1636	arg1	angle					1606:1610	lower (p<0.05) collagen orientation angle	1570:1610	lower (p<0.05) collagen orientation angle at the superficial tissue	1570:1636	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	6	100	theme	depth	1073:1077	arg1	function					1054:1061	a function	1052:1061	a function of tissue depth	1052:1077	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	4	101	theme	Elastic	482:488	arg1	properties					506:515	Elastic and poroelastic properties	482:515	Elastic and poroelastic properties	482:515	Elastic and poroelastic properties were analyzed from experimental stress-relaxation and sinusoidal indentation measurements with fibril reinforced poroelastic finite element modeling.
27987426	3	102	from	meniscus	359:366	arg1	manner					399:404	a site- and depth-dependent manner	371:404	a site- and depth-dependent manner	371:404	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	8	103	dep	higher	1456:1461	arg1	p<0.05					1464:1469	p<0.05	1464:1469	p<0.05	1464:1469	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	3	104	theme	meniscus	359:366	arg1	structure					340:348	structure	340:348	structure	340:348	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	3	104	theme	meniscus	359:366	arg1	composition					324:334	composition	324:334	composition	324:334	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	6	105	contain	had	1106:1108	arg2	increase					1127:1134	an initial sharp increase	1110:1134	an initial sharp increase followed by a decrease across tissue depth	1110:1177	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	6	105	contain	had	1106:1108	arg1	content					1098:1104	the collagen content	1085:1104	the collagen content	1085:1104	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	3	106	with	meniscus	359:366	arg1	properties					470:479	tissue site-specific biomechanical properties	435:479	tissue site-specific biomechanical properties	435:479	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	9	107	theme	middle	1740:1745	arg1	meniscus					1767:1774	the medial meniscus	1756:1774	the medial meniscus	1756:1774	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	107	theme	middle	1740:1745	arg1	sites					1747:1751	anterior vs. middle sites	1727:1751	anterior vs. middle sites of the medial meniscus	1727:1774	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	5	108	theme	collagen	689:696	arg1	angle					744:748	the collagen orientation angle	719:748	the collagen orientation angle	719:748	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	5	108	theme	collagen	689:696	arg1	contents					698:705	Proteoglycan (PG) and collagen contents	667:705	Proteoglycan (PG) and collagen contents	667:705	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	5	108	theme	collagen	689:696	arg1	function					772:779	a function	770:779	a function of tissue depth using microscopic and spectroscopic methods	770:839	Proteoglycan (PG) and collagen contents, as well as the collagen orientation angle, were determined as a function of tissue depth using microscopic and spectroscopic methods, and they were compared with biomechanical properties.
27987426	11	109	theme	collagen	2337:2344	arg1	content					2346:2352	higher collagen content	2330:2352	higher collagen content at all tissue depths and superficial collagen fibril orientation	2330:2417	These nonlinear biomechanical properties are suggested to be further amplified by higher collagen content at all tissue depths and superficial collagen fibril orientation.
27987426	10	110	theme	increased	2180:2188	arg1	content					2193:2199	the increased PG content	2176:2199	the increased PG content	2176:2199	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	10	111	theme	nonlinear	1999:2007	arg1	properties					2023:2032	nonlinear biomechanical properties	1999:2032	nonlinear biomechanical properties	1999:2032	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	7	112	theme	elastic	1211:1217	arg1	parameters					1219:1228	elastic parameters	1211:1228	elastic parameters (equilibrium and instantaneous moduli)	1211:1267	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	3	113	from	manner	399:404	arg1	structure					340:348	structure	340:348	structure	340:348	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	3	113	from	manner	399:404	arg1	composition					324:334	composition	324:334	composition	324:334	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	1	114	theme	meniscus	86:93	arg1	properties					66:75	Biomechanical properties	52:75	Biomechanical properties of human meniscus	52:93	Biomechanical properties of human meniscus have been shown to be site-specific.
27987426	6	115	theme	sharp	1121:1125	arg1	increase					1127:1134	an initial sharp increase	1110:1134	an initial sharp increase followed by a decrease across tissue depth	1110:1177	For all the measurement sites (anterior, middle and posterior) of lateral and medial menisci (n=26), PG content and collagen orientation angle increased as a function of tissue depth while the collagen content had an initial sharp increase followed by a decrease across tissue depth.
27987426	9	116	theme	medial	1760:1765	arg1	meniscus					1767:1774	the medial meniscus	1756:1774	the medial meniscus	1756:1774	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	8	117	theme	tissue	1552:1557	arg1	depth					1559:1563	the entire tissue depth	1541:1563	the entire tissue depth	1541:1563	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	10	118	theme	increased	2213:2221	arg1	tension					2239:2245	increased collagen fibril tension	2213:2245	increased collagen fibril tension	2213:2245	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	8	119	from	content	1530:1536	arg1	depth					1559:1563	the entire tissue depth	1541:1563	the entire tissue depth	1541:1563	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	8	119	from	content	1530:1536	arg1	meniscus					1495:1502	the deep meniscus	1486:1502	the deep meniscus	1486:1502	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	8	120	from	angle	1606:1610	arg1	depth					1559:1563	the entire tissue depth	1541:1563	the entire tissue depth	1541:1563	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	8	120	from	angle	1606:1610	arg1	meniscus					1495:1502	the deep meniscus	1486:1502	the deep meniscus	1486:1502	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	4	121	theme	element	649:655	arg1	modeling					657:664	fibril reinforced poroelastic finite element modeling	612:664	fibril reinforced poroelastic finite element modeling	612:664	Elastic and poroelastic properties were analyzed from experimental stress-relaxation and sinusoidal indentation measurements with fibril reinforced poroelastic finite element modeling.
27987426	11	122	theme	tissue	2361:2366	arg1	depths					2368:2373	all tissue depths	2357:2373	all tissue depths	2357:2373	These nonlinear biomechanical properties are suggested to be further amplified by higher collagen content at all tissue depths and superficial collagen fibril orientation.
27987426	0	123	theme	human	36:40	arg1	meniscus					42:49	human meniscus	36:49	human meniscus	36:49	Structure-function relationships of human meniscus.
27987426	8	124	dep	higher	1505:1510	arg1	p<0.05					1513:1518	p<0.05	1513:1518	p<0.05	1513:1518	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	9	125	theme	higher	1791:1796	arg1	comparisons					1706:1716	certain comparisons	1698:1716	certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle	1698:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	125	theme	higher	1791:1796	arg1	content					1816:1822	significantly higher (p<0.05) collagen content	1777:1822	significantly higher (p<0.05) collagen content	1777:1822	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	8	126	theme	lower	1570:1574	arg1	angle					1606:1610	lower (p<0.05) collagen orientation angle	1570:1610	lower (p<0.05) collagen orientation angle at the superficial tissue	1570:1636	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	9	127	dep	comparisons	1706:1716	arg1	comparisons					1706:1716	certain comparisons	1698:1716	certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle	1698:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	127	dep	comparisons	1706:1716	arg1	content					1816:1822	significantly higher (p<0.05) collagen content	1777:1822	significantly higher (p<0.05) collagen content	1777:1822	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	127	dep	comparisons	1706:1716	arg1	angle					1846:1850	lower orientation angle	1828:1850	lower orientation angle	1828:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	127	dep	comparisons	1706:1716	arg1	such					1719:1722	such	1719:1722	such	1719:1722	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	7	128	theme	highest	1184:1190	arg1	p<0.05					1200:1205	p<0.05	1200:1205	p<0.05	1200:1205	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	128	theme	highest	1184:1190	arg1	values					1192:1197	The highest values	1180:1197	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability)	1180:1372	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	10	129	theme	meniscus	2037:2044	arg1	properties					2023:2032	nonlinear biomechanical properties	1999:2032	nonlinear biomechanical properties	1999:2032	This study suggests that nonlinear biomechanical properties of meniscus, caused by the collagen network and fluid, may be strongly influenced by tissue osmotic swelling from the deep meniscus caused by the increased PG content, leading to increased collagen fibril tension.
27987426	3	130	theme	site-specific	442:454	arg1	properties					470:479	tissue site-specific biomechanical properties	435:479	tissue site-specific biomechanical properties	435:479	Therefore, we investigated the composition and structure of human meniscus in a site- and depth-dependent manner and their relationships with tissue site-specific biomechanical properties.
27987426	4	131	theme	fibril	612:617	arg1	element					649:655	fibril reinforced poroelastic finite element	612:655	fibril reinforced poroelastic finite element modeling	612:664	Elastic and poroelastic properties were analyzed from experimental stress-relaxation and sinusoidal indentation measurements with fibril reinforced poroelastic finite element modeling.
27987426	2	132	theme	meniscus	163:170	arg1	constituents					172:183	meniscus constituents	163:183	meniscus constituents at different depths and locations	163:217	However, it is not known which meniscus constituents at different depths and locations contribute to biomechanical properties obtained from indentation testing.
27987426	7	133	theme	parameters	1219:1228	arg1	p<0.05					1200:1205	p<0.05	1200:1205	p<0.05	1200:1205	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	133	theme	parameters	1219:1228	arg1	values					1192:1197	The highest values	1180:1197	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability)	1180:1372	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	134	theme	fibril	1333:1338	arg1	modulus					1348:1354	strain-dependent fibril network modulus	1316:1354	strain-dependent fibril network modulus	1316:1354	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	134	theme	fibril	1333:1338	arg1	parameters					1304:1313	strain-dependent biomechanical parameters	1273:1313	strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability)	1273:1372	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	8	135	contain	had	1447:1449	arg2	content					1475:1481	also higher (p<0.05) PG content	1451:1481	also higher (p<0.05) PG content in the deep meniscus	1451:1502	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	8	135	contain	had	1447:1449	arg1	location					1438:1445	This location	1433:1445	This location	1433:1445	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	8	135	contain	had	1447:1449	arg2	content					1530:1536	higher (p<0.05) collagen content	1505:1536	higher (p<0.05) collagen content in the entire tissue depth	1505:1563	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	8	135	contain	had	1447:1449	arg2	angle					1606:1610	lower (p<0.05) collagen orientation angle	1570:1610	lower (p<0.05) collagen orientation angle at the superficial tissue	1570:1636	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	8	136	theme	collagen	1585:1592	arg1	angle					1606:1610	lower (p<0.05) collagen orientation angle	1570:1610	lower (p<0.05) collagen orientation angle at the superficial tissue	1570:1636	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	7	137	dep	parameters	1219:1228	arg1	equilibrium					1231:1241	equilibrium	1231:1241	equilibrium	1231:1241	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	137	dep	parameters	1219:1228	arg1	moduli					1261:1266	instantaneous moduli	1247:1266	instantaneous moduli	1247:1266	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	11	138	from	depths	2368:2373	arg1	content					2346:2352	higher collagen content	2330:2352	higher collagen content at all tissue depths and superficial collagen fibril orientation	2330:2417	These nonlinear biomechanical properties are suggested to be further amplified by higher collagen content at all tissue depths and superficial collagen fibril orientation.
27987426	8	139	theme	other	1659:1663	arg1	locations					1665:1673	many other locations	1654:1673	many other locations	1654:1673	This location had also higher (p<0.05) PG content in the deep meniscus, higher (p<0.05) collagen content in the entire tissue depth, and lower (p<0.05) collagen orientation angle at the superficial tissue, as compared to many other locations.
27987426	1	140	theme	Biomechanical	52:64	arg1	properties					66:75	Biomechanical properties	52:75	Biomechanical properties of human meniscus	52:93	Biomechanical properties of human meniscus have been shown to be site-specific.
27987426	7	141	theme	biomechanical	1290:1302	arg1	permeability					1360:1371	permeability	1360:1371	permeability	1360:1371	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	141	theme	biomechanical	1290:1302	arg1	modulus					1348:1354	strain-dependent fibril network modulus	1316:1354	strain-dependent fibril network modulus	1316:1354	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	7	141	theme	biomechanical	1290:1302	arg1	parameters					1304:1313	strain-dependent biomechanical parameters	1273:1313	strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability)	1273:1372	The highest values (p<0.05) of elastic parameters (equilibrium and instantaneous moduli) and strain-dependent biomechanical parameters (strain-dependent fibril network modulus and permeability) were observed in the anterior horn of the medial meniscus.
27987426	9	142	theme	orientation	1834:1844	arg1	comparisons					1706:1716	certain comparisons	1698:1716	certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle	1698:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
27987426	9	142	theme	orientation	1834:1844	arg1	angle					1846:1850	lower orientation angle	1828:1850	lower orientation angle	1828:1850	On the other hand, in certain comparisons (such as anterior vs. middle sites of the medial meniscus) significantly higher (p<0.05) collagen content and lower orientation angle, without any difference in the PG content, were consistent with increased meniscus modulus and/or nonlinear permeability.
25410683	2	0	theme	protein	533:539	arg1	characteristics					385:399	The main compositional characteristics	362:399	The main compositional characteristics of this cheese after 60 days of ripening	362:440	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	0	theme	protein	533:539	arg1	RESULTS					354:360	RESULTS	354:360	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).	354:638	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	0	theme	protein	533:539	arg1	proportion					456:465	its high proportion	447:465	its high proportion of total solids (TS; 752 g kg⁻¹ of cheese)	447:508	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	0	theme	protein	533:539	arg1	presence					593:600	the presence	589:600	the presence of residual lactose (12.5 g kg⁻¹ TS)	589:637	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	0	theme	protein	533:539	arg1	content					522:528	an average content	511:528	an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS)	511:583	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	6	1	theme	fat	1036:1038	arg1	kg⁻¹					1028:1031	50.1 mg KOH kg⁻¹	1016:1031	50.1 mg KOH kg⁻¹ of fat	1016:1038	Fat acidity increased during ripening, reaching final values of 50.1 mg KOH kg⁻¹ of fat.
25410683	9	2	theme	calcium	1245:1251	arg1	content					1253:1259	low calcium content	1241:1259	low calcium content	1241:1259	The low pH, low calcium content and limited proteolysis led to a crumbly texture in this cheese variety.
25410683	1	3	theme	traditional	239:249	arg1	variety					259:265	a traditional Spanish variety	237:265	a traditional Spanish variety	237:265	BACKGROUND Physicochemical characteristics, proteolysis and lipolysis were studied throughout the ripening of eight batches of a traditional Spanish variety made from raw cow's milk, in order to establish a basis for its industrial production.
25410683	0	4	theme	raw	69:71	arg1	cow					73:75	Spanish raw cow's	61:77	a Spanish raw cow's milk variety	59:90	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.
25410683	4	5	theme	g	696:696	arg1	TS					703:704	5.13 g kg⁻¹ TS	691:704	5.13 g kg⁻¹ TS	691:704	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	5	theme	g	696:696	arg1	Phosphorus					679:688	Phosphorus	679:688	Phosphorus (5.13 g kg⁻¹ TS)	679:705	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	2	6	theme	fat	563:565	arg1	characteristics					385:399	The main compositional characteristics	362:399	The main compositional characteristics of this cheese after 60 days of ripening	362:440	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	6	theme	fat	563:565	arg1	RESULTS					354:360	RESULTS	354:360	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).	354:638	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	6	theme	fat	563:565	arg1	proportion					456:465	its high proportion	447:465	its high proportion of total solids (TS; 752 g kg⁻¹ of cheese)	447:508	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	6	theme	fat	563:565	arg1	presence					593:600	the presence	589:600	the presence of residual lactose (12.5 g kg⁻¹ TS)	589:637	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	6	theme	fat	563:565	arg1	content					522:528	an average content	511:528	an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS)	511:583	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	6	7	theme	mg	1021:1022	arg1	kg⁻¹					1028:1031	50.1 mg KOH kg⁻¹	1016:1031	50.1 mg KOH kg⁻¹ of fat	1016:1038	Fat acidity increased during ripening, reaching final values of 50.1 mg KOH kg⁻¹ of fat.
25410683	1	8	theme	Spanish	251:257	arg1	variety					259:265	a traditional Spanish variety	237:265	a traditional Spanish variety	237:265	BACKGROUND Physicochemical characteristics, proteolysis and lipolysis were studied throughout the ripening of eight batches of a traditional Spanish variety made from raw cow's milk, in order to establish a basis for its industrial production.
25410683	0	9	theme	milk	79:82	arg1	cheese					51:56	Genestoso cheese	41:56	Genestoso cheese	41:56	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.
25410683	0	9	theme	milk	79:82	arg1	variety					84:90	a Spanish raw cow's milk variety	59:90	a Spanish raw cow's milk variety	59:90	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.
25410683	6	10	theme	KOH	1024:1026	arg1	kg⁻¹					1028:1031	50.1 mg KOH kg⁻¹	1016:1031	50.1 mg KOH kg⁻¹ of fat	1016:1038	Fat acidity increased during ripening, reaching final values of 50.1 mg KOH kg⁻¹ of fat.
25410683	1	11	theme	variety	259:265	arg1	batches					226:232	eight batches	220:232	eight batches of a traditional Spanish variety made from raw cow's milk	220:290	BACKGROUND Physicochemical characteristics, proteolysis and lipolysis were studied throughout the ripening of eight batches of a traditional Spanish variety made from raw cow's milk, in order to establish a basis for its industrial production.
25410683	2	12	theme	average	514:520	arg1	content					522:528	an average content	511:528	an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS)	511:583	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	12	theme	average	514:520	arg1	RESULTS					354:360	RESULTS	354:360	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).	354:638	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	9	13	theme	limited	1265:1271	arg1	proteolysis					1273:1283	limited proteolysis	1265:1283	limited proteolysis	1265:1283	The low pH, low calcium content and limited proteolysis led to a crumbly texture in this cheese variety.
25410683	2	14	theme	kg⁻¹	630:633	arg1	TS					635:636	12.5 g kg⁻¹ TS	623:636	12.5 g kg⁻¹ TS	623:636	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	14	theme	kg⁻¹	630:633	arg1	lactose					614:620	residual lactose	605:620	residual lactose (12.5 g kg⁻¹ TS)	605:637	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	4	15	theme	abundant	749:756	arg1	elements					766:773	the most abundant mineral elements	740:773	the most abundant mineral elements in cheese	740:783	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	15	theme	abundant	749:756	arg1	Phosphorus					679:688	Phosphorus	679:688	Phosphorus (5.13 g kg⁻¹ TS)	679:705	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	15	theme	abundant	749:756	arg1	sodium					711:716	sodium	711:716	sodium (8.29 g kg⁻¹ TS)	711:733	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	2	16	theme	kg⁻¹	550:553	arg1	TS					555:556	452.8 g kg⁻¹ TS	542:556	452.8 g kg⁻¹ TS	542:556	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	16	theme	kg⁻¹	550:553	arg1	protein					533:539	protein	533:539	protein (452.8 g kg⁻¹ TS)	533:557	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	17	theme	g	628:628	arg1	TS					635:636	12.5 g kg⁻¹ TS	623:636	12.5 g kg⁻¹ TS	623:636	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	17	theme	g	628:628	arg1	lactose					614:620	residual lactose	605:620	residual lactose (12.5 g kg⁻¹ TS)	605:637	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	18	theme	main	366:369	arg1	characteristics					385:399	The main compositional characteristics	362:399	The main compositional characteristics of this cheese after 60 days of ripening	362:440	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	18	theme	main	366:369	arg1	proportion					456:465	its high proportion	447:465	its high proportion of total solids (TS; 752 g kg⁻¹ of cheese)	447:508	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	19	theme	solids	476:481	arg1	characteristics					385:399	The main compositional characteristics	362:399	The main compositional characteristics of this cheese after 60 days of ripening	362:440	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	19	theme	solids	476:481	arg1	RESULTS					354:360	RESULTS	354:360	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).	354:638	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	19	theme	solids	476:481	arg1	proportion					456:465	its high proportion	447:465	its high proportion of total solids (TS; 752 g kg⁻¹ of cheese)	447:508	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	19	theme	solids	476:481	arg1	presence					593:600	the presence	589:600	the presence of residual lactose (12.5 g kg⁻¹ TS)	589:637	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	19	theme	solids	476:481	arg1	content					522:528	an average content	511:528	an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS)	511:583	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	1	20	theme	industrial	331:340	arg1	production					342:351	its industrial production	327:351	its industrial production	327:351	BACKGROUND Physicochemical characteristics, proteolysis and lipolysis were studied throughout the ripening of eight batches of a traditional Spanish variety made from raw cow's milk, in order to establish a basis for its industrial production.
25410683	2	21	theme	g	548:548	arg1	TS					555:556	452.8 g kg⁻¹ TS	542:556	452.8 g kg⁻¹ TS	542:556	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	21	theme	g	548:548	arg1	protein					533:539	protein	533:539	protein (452.8 g kg⁻¹ TS)	533:557	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	22	theme	kg⁻¹	576:579	arg1	TS					581:582	475.1 g kg⁻¹ TS	568:582	475.1 g kg⁻¹ TS	568:582	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	22	theme	kg⁻¹	576:579	arg1	fat					563:565	fat	563:565	fat (475.1 g kg⁻¹ TS)	563:583	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	23	theme	high	451:454	arg1	characteristics					385:399	The main compositional characteristics	362:399	The main compositional characteristics of this cheese after 60 days of ripening	362:440	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	23	theme	high	451:454	arg1	proportion					456:465	its high proportion	447:465	its high proportion of total solids (TS; 752 g kg⁻¹ of cheese)	447:508	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	23	theme	high	451:454	arg1	RESULTS					354:360	RESULTS	354:360	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).	354:638	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	4	24	theme	5.13	691:694	arg1	TS					703:704	5.13 g kg⁻¹ TS	691:704	5.13 g kg⁻¹ TS	691:704	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	24	theme	5.13	691:694	arg1	Phosphorus					679:688	Phosphorus	679:688	Phosphorus (5.13 g kg⁻¹ TS)	679:705	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	2	25	theme	g	574:574	arg1	TS					581:582	475.1 g kg⁻¹ TS	568:582	475.1 g kg⁻¹ TS	568:582	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	25	theme	g	574:574	arg1	fat					563:565	fat	563:565	fat (475.1 g kg⁻¹ TS)	563:583	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	7	26	theme	free	1050:1053	arg1	C16:0					1070:1074	C16:0	1070:1074	C16:0	1070:1074	The main free fatty acid was C16:0, followed by C18:1 and C14:0.
25410683	7	26	theme	free	1050:1053	arg1	acid					1061:1064	The main free fatty acid	1041:1064	The main free fatty acid	1041:1064	The main free fatty acid was C16:0, followed by C18:1 and C14:0.
25410683	0	27	theme	Compositional	0:12	arg1	changes					30:36	Compositional and biochemical changes	0:36	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.	0:108	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.
25410683	6	28	theme	final	1000:1004	arg1	values					1006:1011	final values	1000:1011	final values of 50.1 mg KOH kg⁻¹ of fat	1000:1038	Fat acidity increased during ripening, reaching final values of 50.1 mg KOH kg⁻¹ of fat.
25410683	6	29	theme	50.1	1016:1019	arg1	mg					1021:1022	mg	1021:1022	mg	1021:1022	Fat acidity increased during ripening, reaching final values of 50.1 mg KOH kg⁻¹ of fat.
25410683	6	30	theme	kg⁻¹	1028:1031	arg1	values					1006:1011	final values	1000:1011	final values of 50.1 mg KOH kg⁻¹ of fat	1000:1038	Fat acidity increased during ripening, reaching final values of 50.1 mg KOH kg⁻¹ of fat.
25410683	4	31	theme	kg⁻¹	817:820	arg1	TS					822:823	1.92 g kg⁻¹ TS	810:823	1.92 g kg⁻¹ TS	810:823	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	31	theme	kg⁻¹	817:820	arg1	levels					802:807	calcium levels	794:807	calcium levels (1.92 g kg⁻¹ TS)	794:824	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	1	32	theme	raw	277:279	arg1	cow					281:283	raw cow's	277:285	raw cow's milk	277:290	BACKGROUND Physicochemical characteristics, proteolysis and lipolysis were studied throughout the ripening of eight batches of a traditional Spanish variety made from raw cow's milk, in order to establish a basis for its industrial production.
25410683	4	33	theme	kg⁻¹	726:729	arg1	TS					731:732	8.29 g kg⁻¹ TS	719:732	8.29 g kg⁻¹ TS	719:732	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	33	theme	kg⁻¹	726:729	arg1	sodium					711:716	sodium	711:716	sodium (8.29 g kg⁻¹ TS)	711:733	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	9	34	theme	crumbly	1294:1300	arg1	texture					1302:1308	a crumbly texture	1292:1308	a crumbly texture in this cheese variety	1292:1331	The low pH, low calcium content and limited proteolysis led to a crumbly texture in this cheese variety.
25410683	0	35	theme	biochemical	18:28	arg1	changes					30:36	Compositional and biochemical changes	0:36	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.	0:108	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.
25410683	2	36	dep	solids	476:481	arg1	TS					484:485	TS	484:485	TS; 752 g kg⁻¹ of cheese	484:507	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	8	37	theme	limited	1169:1175	arg1	proteolysis					1177:1187	a limited proteolysis	1167:1187	a limited proteolysis	1167:1187	CONCLUSION These results suggest that this variety undergoes a limited proteolysis and moderate lipolysis during ripening.
25410683	8	38	dep	CONCLUSION	1106:1115	arg1	suggest					1131:1137	suggest	1131:1137	suggest that this variety undergoes a limited proteolysis and moderate lipolysis during ripening	1131:1226	CONCLUSION These results suggest that this variety undergoes a limited proteolysis and moderate lipolysis during ripening.
25410683	7	39	theme	main	1045:1048	arg1	C16:0					1070:1074	C16:0	1070:1074	C16:0	1070:1074	The main free fatty acid was C16:0, followed by C18:1 and C14:0.
25410683	7	39	theme	main	1045:1048	arg1	acid					1061:1064	The main free fatty acid	1041:1064	The main free fatty acid	1041:1064	The main free fatty acid was C16:0, followed by C18:1 and C14:0.
25410683	0	40	theme	Genestoso	41:49	arg1	variety					84:90	a Spanish raw cow's milk variety	59:90	a Spanish raw cow's milk variety	59:90	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.
25410683	0	40	theme	Genestoso	41:49	arg1	cheese					51:56	Genestoso cheese	41:56	Genestoso cheese	41:56	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.
25410683	5	41	theme	β-casein	942:949	arg1	degradation					918:928	almost zero degradation	906:928	almost zero degradation of αs1- and β-casein	906:949	Proteolysis extension and depth were very low, which resulted in almost zero degradation of αs1- and β-casein.
25410683	2	42	theme	g	492:492	arg1	kg⁻¹					494:497	752 g kg⁻¹	488:497	TS; 752 g kg⁻¹ of cheese	484:507	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	7	43	theme	fatty	1055:1059	arg1	C16:0					1070:1074	C16:0	1070:1074	C16:0	1070:1074	The main free fatty acid was C16:0, followed by C18:1 and C14:0.
25410683	7	43	theme	fatty	1055:1059	arg1	acid					1061:1064	The main free fatty acid	1041:1064	The main free fatty acid	1041:1064	The main free fatty acid was C16:0, followed by C18:1 and C14:0.
25410683	2	44	dep	TS	484:485	arg1	kg⁻¹					494:497	752 g kg⁻¹	488:497	TS; 752 g kg⁻¹ of cheese	484:507	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	5	45	theme	Proteolysis	841:851	arg1	extension					853:861	Proteolysis extension	841:861	Proteolysis extension	841:861	Proteolysis extension and depth were very low, which resulted in almost zero degradation of αs1- and β-casein.
25410683	4	46	from	elements	766:773	arg1	cheese					778:783	cheese	778:783	cheese	778:783	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	2	47	theme	compositional	371:383	arg1	characteristics					385:399	The main compositional characteristics	362:399	The main compositional characteristics of this cheese after 60 days of ripening	362:440	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	47	theme	compositional	371:383	arg1	proportion					456:465	its high proportion	447:465	its high proportion of total solids (TS; 752 g kg⁻¹ of cheese)	447:508	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	48	theme	ripening	433:440	arg1	days					425:428	60 days	422:428	60 days of ripening	422:440	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	49	theme	total	470:474	arg1	solids					476:481	total solids	470:481	total solids (TS; 752 g kg⁻¹ of cheese)	470:508	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	5	50	theme	αs1-	933:936	arg1	degradation					918:928	almost zero degradation	906:928	almost zero degradation of αs1- and β-casein	906:949	Proteolysis extension and depth were very low, which resulted in almost zero degradation of αs1- and β-casein.
25410683	9	51	theme	cheese	1318:1323	arg1	variety					1325:1331	this cheese variety	1313:1331	this cheese variety	1313:1331	The low pH, low calcium content and limited proteolysis led to a crumbly texture in this cheese variety.
25410683	4	52	dep	elements	766:773	arg1	whereas					786:792	whereas	786:792	whereas	786:792	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	53	theme	calcium	794:800	arg1	TS					822:823	1.92 g kg⁻¹ TS	810:823	1.92 g kg⁻¹ TS	810:823	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	53	theme	calcium	794:800	arg1	levels					802:807	calcium levels	794:807	calcium levels (1.92 g kg⁻¹ TS)	794:824	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	8	54	theme	moderate	1193:1200	arg1	lipolysis					1202:1210	moderate lipolysis	1193:1210	moderate lipolysis	1193:1210	CONCLUSION These results suggest that this variety undergoes a limited proteolysis and moderate lipolysis during ripening.
25410683	4	55	theme	8.29	719:722	arg1	TS					731:732	8.29 g kg⁻¹ TS	719:732	8.29 g kg⁻¹ TS	719:732	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	55	theme	8.29	719:722	arg1	sodium					711:716	sodium	711:716	sodium (8.29 g kg⁻¹ TS)	711:733	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	56	theme	g	815:815	arg1	TS					822:823	1.92 g kg⁻¹ TS	810:823	1.92 g kg⁻¹ TS	810:823	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	56	theme	g	815:815	arg1	levels					802:807	calcium levels	794:807	calcium levels (1.92 g kg⁻¹ TS)	794:824	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	2	57	dep	RESULTS	354:360	arg1	characteristics					385:399	The main compositional characteristics	362:399	The main compositional characteristics of this cheese after 60 days of ripening	362:440	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	57	dep	RESULTS	354:360	arg1	RESULTS					354:360	RESULTS	354:360	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).	354:638	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	57	dep	RESULTS	354:360	arg1	proportion					456:465	its high proportion	447:465	its high proportion of total solids (TS; 752 g kg⁻¹ of cheese)	447:508	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	57	dep	RESULTS	354:360	arg1	presence					593:600	the presence	589:600	the presence of residual lactose (12.5 g kg⁻¹ TS)	589:637	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	57	dep	RESULTS	354:360	arg1	content					522:528	an average content	511:528	an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS)	511:583	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	9	58	theme	low	1233:1235	arg1	pH					1237:1238	The low pH	1229:1238	The low pH	1229:1238	The low pH, low calcium content and limited proteolysis led to a crumbly texture in this cheese variety.
25410683	9	59	from	texture	1302:1308	arg1	variety					1325:1331	this cheese variety	1313:1331	this cheese variety	1313:1331	The low pH, low calcium content and limited proteolysis led to a crumbly texture in this cheese variety.
25410683	2	60	theme	cheese	502:507	arg1	kg⁻¹					494:497	752 g kg⁻¹	488:497	TS; 752 g kg⁻¹ of cheese	484:507	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	61	theme	lactose	614:620	arg1	characteristics					385:399	The main compositional characteristics	362:399	The main compositional characteristics of this cheese after 60 days of ripening	362:440	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	61	theme	lactose	614:620	arg1	RESULTS					354:360	RESULTS	354:360	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).	354:638	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	61	theme	lactose	614:620	arg1	proportion					456:465	its high proportion	447:465	its high proportion of total solids (TS; 752 g kg⁻¹ of cheese)	447:508	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	61	theme	lactose	614:620	arg1	presence					593:600	the presence	589:600	the presence of residual lactose (12.5 g kg⁻¹ TS)	589:637	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	61	theme	lactose	614:620	arg1	content					522:528	an average content	511:528	an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS)	511:583	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	1	62	theme	BACKGROUND	110:119	arg1	characteristics					137:151	BACKGROUND Physicochemical characteristics	110:151	BACKGROUND Physicochemical characteristics	110:151	BACKGROUND Physicochemical characteristics, proteolysis and lipolysis were studied throughout the ripening of eight batches of a traditional Spanish variety made from raw cow's milk, in order to establish a basis for its industrial production.
25410683	4	63	theme	g	724:724	arg1	TS					731:732	8.29 g kg⁻¹ TS	719:732	8.29 g kg⁻¹ TS	719:732	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	63	theme	g	724:724	arg1	sodium					711:716	sodium	711:716	sodium (8.29 g kg⁻¹ TS)	711:733	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	1	64	theme	batches	226:232	arg1	ripening					208:215	the ripening	204:215	the ripening of eight batches of a traditional Spanish variety made from raw cow's milk	204:290	BACKGROUND Physicochemical characteristics, proteolysis and lipolysis were studied throughout the ripening of eight batches of a traditional Spanish variety made from raw cow's milk, in order to establish a basis for its industrial production.
25410683	0	65	theme	Spanish	61:67	arg1	cow					73:75	Spanish raw cow's	61:77	a Spanish raw cow's milk variety	59:90	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.
25410683	6	66	theme	Fat	952:954	arg1	acidity					956:962	Fat acidity	952:962	Fat acidity	952:962	Fat acidity increased during ripening, reaching final values of 50.1 mg KOH kg⁻¹ of fat.
25410683	2	67	theme	residual	605:612	arg1	TS					635:636	12.5 g kg⁻¹ TS	623:636	12.5 g kg⁻¹ TS	623:636	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	67	theme	residual	605:612	arg1	lactose					614:620	residual lactose	605:620	residual lactose (12.5 g kg⁻¹ TS)	605:637	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	1	68	theme	Physicochemical	121:135	arg1	characteristics					137:151	BACKGROUND Physicochemical characteristics	110:151	BACKGROUND Physicochemical characteristics	110:151	BACKGROUND Physicochemical characteristics, proteolysis and lipolysis were studied throughout the ripening of eight batches of a traditional Spanish variety made from raw cow's milk, in order to establish a basis for its industrial production.
25410683	3	69	theme	pH	644:645	arg1	4.04					654:657	4.04	654:657	4.04	654:657	Its pH value (4.04) was extremely low.
25410683	3	69	theme	pH	644:645	arg1	value					647:651	Its pH value	640:651	Its pH value (4.04)	640:658	Its pH value (4.04) was extremely low.
25410683	4	70	theme	mineral	758:764	arg1	elements					766:773	the most abundant mineral elements	740:773	the most abundant mineral elements in cheese	740:783	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	70	theme	mineral	758:764	arg1	Phosphorus					679:688	Phosphorus	679:688	Phosphorus (5.13 g kg⁻¹ TS)	679:705	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	70	theme	mineral	758:764	arg1	sodium					711:716	sodium	711:716	sodium (8.29 g kg⁻¹ TS)	711:733	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	0	71	from	changes	30:36	arg1	variety					84:90	a Spanish raw cow's milk variety	59:90	a Spanish raw cow's milk variety	59:90	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.
25410683	0	71	from	changes	30:36	arg1	cheese					51:56	Genestoso cheese	41:56	Genestoso cheese	41:56	Compositional and biochemical changes in Genestoso cheese, a Spanish raw cow's milk variety, during ripening.
25410683	2	72	theme	cheese	409:414	arg1	characteristics					385:399	The main compositional characteristics	362:399	The main compositional characteristics of this cheese after 60 days of ripening	362:440	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	2	72	theme	cheese	409:414	arg1	proportion					456:465	its high proportion	447:465	its high proportion of total solids (TS; 752 g kg⁻¹ of cheese)	447:508	RESULTS The main compositional characteristics of this cheese after 60 days of ripening were its high proportion of total solids (TS; 752 g kg⁻¹ of cheese), an average content of protein (452.8 g kg⁻¹ TS) and fat (475.1 g kg⁻¹ TS) and the presence of residual lactose (12.5 g kg⁻¹ TS).
25410683	9	73	theme	low	1241:1243	arg1	content					1253:1259	low calcium content	1241:1259	low calcium content	1241:1259	The low pH, low calcium content and limited proteolysis led to a crumbly texture in this cheese variety.
25410683	4	74	theme	kg⁻¹	698:701	arg1	TS					703:704	5.13 g kg⁻¹ TS	691:704	5.13 g kg⁻¹ TS	691:704	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
25410683	4	74	theme	kg⁻¹	698:701	arg1	Phosphorus					679:688	Phosphorus	679:688	Phosphorus (5.13 g kg⁻¹ TS)	679:705	Phosphorus (5.13 g kg⁻¹ TS) and sodium (8.29 g kg⁻¹ TS) were the most abundant mineral elements in cheese, whereas calcium levels (1.92 g kg⁻¹ TS) were very low.
27312477	0	0	theme	hyaluronic	70:79	arg1	hydrogel					92:99	hyaluronic acid-based hydrogel	70:99	hyaluronic acid-based hydrogel	70:99	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	1	1	theme	low	346:348	arg1	level					350:354	a low level	344:354	a low level of cross-linker	344:370	Regardless of various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE), seeking new strategies that enhance cross-linking efficiency with a low level of cross-linker is essential.
27312477	1	2	theme	various	166:172	arg1	strategies					174:183	various strategies	166:183	various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE)	166:275	Regardless of various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE), seeking new strategies that enhance cross-linking efficiency with a low level of cross-linker is essential.
27312477	2	3	theme	reaction	825:832	arg1	mixture					834:840	one reaction mixture	821:840	one reaction mixture (hydrogel 2)	821:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	2	3	theme	reaction	825:832	arg1	hydrogel					843:850	hydrogel 2	843:852	hydrogel 2	843:852	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	6	4	theme	denser	1335:1340	arg1	structure					1350:1358	a denser network structure	1333:1358	a denser network structure	1333:1358	SEM images demonstrated that hydrogel 2 composite showed a denser network structure and smaller pore-size than hydrogel 1.
27312477	5	5	theme	swelling	1092:1099	arg1	higher					1139:1144	higher	1139:1144	higher	1139:1144	The swelling ratio of hydrogel 1 was significantly higher than that of hydrogel 2 in distilled water; however, in phosphate buffer saline, both hydrogels showed no significant difference.
27312477	5	5	theme	swelling	1092:1099	arg1	ratio					1101:1105	The swelling ratio	1088:1105	The swelling ratio of hydrogel 1	1088:1119	The swelling ratio of hydrogel 1 was significantly higher than that of hydrogel 2 in distilled water; however, in phosphate buffer saline, both hydrogels showed no significant difference.
27312477	7	6	theme	cross-linked	1474:1485	arg1	hydrogels					1487:1495	the cross-linked hydrogels	1470:1495	the cross-linked hydrogels	1470:1495	In comparison to native HA, the occurrence of chemical modification in the cross-linked hydrogels was confirmed by FTIR and NMR distinctive peaks.
27312477	5	7	theme	phosphate	1202:1210	arg1	buffer					1212:1217	phosphate buffer saline	1202:1224	phosphate buffer saline	1202:1224	The swelling ratio of hydrogel 1 was significantly higher than that of hydrogel 2 in distilled water; however, in phosphate buffer saline, both hydrogels showed no significant difference.
27312477	0	8	theme	hydrogel	92:99	arg1	efficiency					56:65	cross-linking efficiency	42:65	cross-linking efficiency of hyaluronic acid-based hydrogel	42:99	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	0	9	from	Study	0:4	arg1	efficiency					56:65	cross-linking efficiency	42:65	cross-linking efficiency of hyaluronic acid-based hydrogel	42:99	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	9	10	theme	HA	1855:1856	arg1	chains					1858:1863	HA chains	1855:1863	HA chains	1855:1863	In conclusion, the small-batches mixing approach proved to be more effective than large-batch mixing in promoting HA-HA entanglement and increasing the probability of BDDE molecules for binding with HA chains.
27312477	0	11	theme	acid-based	81:90	arg1	hydrogel					92:99	hyaluronic acid-based hydrogel	70:99	hyaluronic acid-based hydrogel	70:99	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	1	12	theme	cross-linker	359:370	arg1	level					350:354	a low level	344:354	a low level of cross-linker	344:370	Regardless of various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE), seeking new strategies that enhance cross-linking efficiency with a low level of cross-linker is essential.
27312477	7	13	from	occurrence	1431:1440	arg1	hydrogels					1487:1495	the cross-linked hydrogels	1470:1495	the cross-linked hydrogels	1470:1495	In comparison to native HA, the occurrence of chemical modification in the cross-linked hydrogels was confirmed by FTIR and NMR distinctive peaks.
27312477	4	14	theme	Degradation	936:946	arg1	tests					948:952	Degradation tests	936:952	Degradation tests	936:952	Degradation tests proved that, in relation to hydrogel 1, hydrogel 2 was more stable, and exhibited a higher resistance towards hyaluronidase activity.
27312477	7	15	theme	native	1416:1421	arg1	HA					1423:1424	native HA	1416:1424	native HA	1416:1424	In comparison to native HA, the occurrence of chemical modification in the cross-linked hydrogels was confirmed by FTIR and NMR distinctive peaks.
27312477	2	16	dep	approaches	515:524	arg1	approach					550:557	the large-batch mixing approach	527:557	two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	494:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	9	17	with	binding	1842:1848	arg1	chains					1858:1863	HA chains	1855:1863	HA chains	1855:1863	In conclusion, the small-batches mixing approach proved to be more effective than large-batch mixing in promoting HA-HA entanglement and increasing the probability of BDDE molecules for binding with HA chains.
27312477	9	18	theme	BDDE	1823:1826	arg1	molecules					1828:1836	BDDE molecules	1823:1836	BDDE molecules	1823:1836	In conclusion, the small-batches mixing approach proved to be more effective than large-batch mixing in promoting HA-HA entanglement and increasing the probability of BDDE molecules for binding with HA chains.
27312477	2	19	theme	mixing	508:513	arg1	approaches					515:524	two different mixing approaches	494:524	two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	494:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	9	20	theme	entanglement	1776:1787	arg1	HA-HA					1770:1774	HA-HA entanglement	1770:1787	HA-HA entanglement	1770:1787	In conclusion, the small-batches mixing approach proved to be more effective than large-batch mixing in promoting HA-HA entanglement and increasing the probability of BDDE molecules for binding with HA chains.
27312477	2	21	link	cross-linked	451:462	arg1	hydrogels					472:480	two cross-linked BDDE-HA hydrogels	447:480	two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	447:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	9	22	theme	molecules	1828:1836	arg1	probability					1808:1818	the probability	1804:1818	the probability of BDDE molecules for binding with HA chains	1804:1863	In conclusion, the small-batches mixing approach proved to be more effective than large-batch mixing in promoting HA-HA entanglement and increasing the probability of BDDE molecules for binding with HA chains.
27312477	6	23	theme	hydrogel	1305:1312	arg1	composite					1316:1324	hydrogel 2 composite	1305:1324	hydrogel 2 composite	1305:1324	SEM images demonstrated that hydrogel 2 composite showed a denser network structure and smaller pore-size than hydrogel 1.
27312477	2	24	theme	single	612:617	arg1	approach					684:691	approach	684:691	approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	684:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	2	24	theme	single	612:617	arg1	lump					619:622	a single lump	610:622	a single lump in one container (hydrogel 1)	610:652	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	2	24	theme	single	612:617	arg1	quantities					581:590	the hydrogel quantities	568:590	the hydrogel quantities	568:590	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	6	25	theme	network	1342:1348	arg1	structure					1350:1358	a denser network structure	1333:1358	a denser network structure	1333:1358	SEM images demonstrated that hydrogel 2 composite showed a denser network structure and smaller pore-size than hydrogel 1.
27312477	2	26	theme	approach	435:442	arg1	influence					415:423	the influence	411:423	the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	411:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	4	27	theme	hyaluronidase	1064:1076	arg1	activity					1078:1085	hyaluronidase activity	1064:1085	hyaluronidase activity	1064:1085	Degradation tests proved that, in relation to hydrogel 1, hydrogel 2 was more stable, and exhibited a higher resistance towards hyaluronidase activity.
27312477	1	28	theme	hyaluronic	212:221	arg1	HA					229:230	HA	229:230	HA	229:230	Regardless of various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE), seeking new strategies that enhance cross-linking efficiency with a low level of cross-linker is essential.
27312477	1	28	theme	hyaluronic	212:221	arg1	acid					223:226	hyaluronic acid	212:226	hyaluronic acid (HA)	212:231	Regardless of various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE), seeking new strategies that enhance cross-linking efficiency with a low level of cross-linker is essential.
27312477	2	29	theme	mixing	428:433	arg1	approach					435:442	mixing approach	428:442	mixing approach	428:442	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	1	30	theme	new	286:288	arg1	strategies					290:299	new strategies	286:299	new strategies that enhance cross-linking efficiency with a low level of cross-linker	286:370	Regardless of various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE), seeking new strategies that enhance cross-linking efficiency with a low level of cross-linker is essential.
27312477	0	31	theme	diglycidyl	134:143	arg1	ether					145:149	1,4-butanediol diglycidyl ether	119:149	1,4-butanediol diglycidyl ether	119:149	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	7	32	link	cross-linked	1474:1485	arg1	hydrogels					1487:1495	the cross-linked hydrogels	1470:1495	the cross-linked hydrogels	1470:1495	In comparison to native HA, the occurrence of chemical modification in the cross-linked hydrogels was confirmed by FTIR and NMR distinctive peaks.
27312477	0	33	theme	1,4-butanediol	119:132	arg1	ether					145:149	1,4-butanediol diglycidyl ether	119:149	1,4-butanediol diglycidyl ether	119:149	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	6	34	theme	smaller	1364:1370	arg1	pore-size					1372:1380	smaller pore-size	1364:1380	smaller pore-size	1364:1380	SEM images demonstrated that hydrogel 2 composite showed a denser network structure and smaller pore-size than hydrogel 1.
27312477	3	35	theme	cross-linking	883:895	arg1	reaction					897:904	the cross-linking reaction	879:904	the cross-linking reaction	879:904	The result showed that the cross-linking reaction was mixing process-dependent.
27312477	3	35	theme	cross-linking	883:895	arg1	process-dependent					917:933	process-dependent	917:933	process-dependent	917:933	The result showed that the cross-linking reaction was mixing process-dependent.
27312477	5	36	theme	significant	1252:1262	arg1	difference					1264:1273	no significant difference	1249:1273	no significant difference	1249:1273	The swelling ratio of hydrogel 1 was significantly higher than that of hydrogel 2 in distilled water; however, in phosphate buffer saline, both hydrogels showed no significant difference.
27312477	0	37	theme	mixing	23:28	arg1	approach					30:37	mixing approach	23:37	mixing approach	23:37	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	2	38	theme	mixing	543:548	arg1	approach					550:557	the large-batch mixing approach	527:557	two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	494:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	2	39	theme	smaller	744:750	arg1	batches					752:758	smaller batches	744:758	smaller batches	744:758	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	2	40	theme	BDDE-HA	464:470	arg1	hydrogels					472:480	two cross-linked BDDE-HA hydrogels	447:480	two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	447:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	2	41	theme	large-batch	531:541	arg1	approach					550:557	the large-batch mixing approach	527:557	two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	494:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	0	42	from	efficiency	56:65	arg1	Study					0:4	Study	0:4	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel	0:99	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	0	43	theme	effect	13:18	arg1	Study					0:4	Study	0:4	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel	0:99	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	9	44	theme	large-batch	1738:1748	arg1	mixing					1750:1755	large-batch mixing	1738:1755	large-batch mixing	1738:1755	In conclusion, the small-batches mixing approach proved to be more effective than large-batch mixing in promoting HA-HA entanglement and increasing the probability of BDDE molecules for binding with HA chains.
27312477	2	45	theme	cross-linked	451:462	arg1	hydrogels					472:480	two cross-linked BDDE-HA hydrogels	447:480	two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	447:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	1	46	theme	cross-linking	314:326	arg1	efficiency					328:337	cross-linking efficiency	314:337	cross-linking efficiency	314:337	Regardless of various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE), seeking new strategies that enhance cross-linking efficiency with a low level of cross-linker is essential.
27312477	6	47	theme	SEM	1276:1278	arg1	images					1280:1285	SEM images	1276:1285	SEM images	1276:1285	SEM images demonstrated that hydrogel 2 composite showed a denser network structure and smaller pore-size than hydrogel 1.
27312477	0	48	theme	approach	30:37	arg1	effect					13:18	the effect	9:18	the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel	9:99	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	4	49	from	relation	970:977	arg1	stable					1014:1019	stable	1014:1019	stable	1014:1019	Degradation tests proved that, in relation to hydrogel 1, hydrogel 2 was more stable, and exhibited a higher resistance towards hyaluronidase activity.
27312477	2	50	theme	hydrogel	572:579	arg1	approach					684:691	approach	684:691	approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	684:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	2	50	theme	hydrogel	572:579	arg1	lump					619:622	a single lump	610:622	a single lump in one container (hydrogel 1)	610:652	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	2	50	theme	hydrogel	572:579	arg1	quantities					581:590	the hydrogel quantities	568:590	the hydrogel quantities	568:590	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	7	51	theme	modification	1454:1465	arg1	occurrence					1431:1440	the occurrence	1427:1440	the occurrence of chemical modification in the cross-linked hydrogels	1427:1495	In comparison to native HA, the occurrence of chemical modification in the cross-linked hydrogels was confirmed by FTIR and NMR distinctive peaks.
27312477	2	52	theme	HA/BDDE	789:795	arg1	ratios					797:802	various HA/BDDE ratios	781:802	various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	781:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	2	53	from	lump	619:622	arg1	hydrogel					642:649	hydrogel 1	642:651	hydrogel 1	642:651	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	2	53	from	lump	619:622	arg1	container					631:639	one container	627:639	one container (hydrogel 1)	627:652	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	9	54	theme	small-batches	1675:1687	arg1	effective					1723:1731	effective	1723:1731	effective	1723:1731	In conclusion, the small-batches mixing approach proved to be more effective than large-batch mixing in promoting HA-HA entanglement and increasing the probability of BDDE molecules for binding with HA chains.
27312477	9	54	theme	small-batches	1675:1687	arg1	approach					1696:1703	the small-batches mixing approach	1671:1703	the small-batches mixing approach	1671:1703	In conclusion, the small-batches mixing approach proved to be more effective than large-batch mixing in promoting HA-HA entanglement and increasing the probability of BDDE molecules for binding with HA chains.
27312477	2	55	theme	various	781:787	arg1	ratios					797:802	various HA/BDDE ratios	781:802	various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	781:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	5	56	theme	distilled	1173:1181	arg1	water					1183:1187	distilled water	1173:1187	distilled water	1173:1187	The swelling ratio of hydrogel 1 was significantly higher than that of hydrogel 2 in distilled water; however, in phosphate buffer saline, both hydrogels showed no significant difference.
27312477	9	57	theme	mixing	1689:1694	arg1	effective					1723:1731	effective	1723:1731	effective	1723:1731	In conclusion, the small-batches mixing approach proved to be more effective than large-batch mixing in promoting HA-HA entanglement and increasing the probability of BDDE molecules for binding with HA chains.
27312477	9	57	theme	mixing	1689:1694	arg1	approach					1696:1703	the small-batches mixing approach	1671:1703	the small-batches mixing approach	1671:1703	In conclusion, the small-batches mixing approach proved to be more effective than large-batch mixing in promoting HA-HA entanglement and increasing the probability of BDDE molecules for binding with HA chains.
27312477	7	58	dep	FTIR	1514:1517	arg1	peaks					1539:1543	distinctive peaks	1527:1543	distinctive peaks	1527:1543	In comparison to native HA, the occurrence of chemical modification in the cross-linked hydrogels was confirmed by FTIR and NMR distinctive peaks.
27312477	2	59	theme	hydrogel	706:713	arg1	quantities					715:724	the hydrogel quantities	702:724	the hydrogel quantities	702:724	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	4	60	theme	higher	1038:1043	arg1	resistance					1045:1054	a higher resistance	1036:1054	a higher resistance towards hyaluronidase activity	1036:1085	Degradation tests proved that, in relation to hydrogel 1, hydrogel 2 was more stable, and exhibited a higher resistance towards hyaluronidase activity.
27312477	1	61	theme	1,4-butanediol	238:251	arg1	BDDE					271:274	BDDE	271:274	BDDE	271:274	Regardless of various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE), seeking new strategies that enhance cross-linking efficiency with a low level of cross-linker is essential.
27312477	1	61	theme	1,4-butanediol	238:251	arg1	ether					264:268	1,4-butanediol diglycidyl ether	238:268	1,4-butanediol diglycidyl ether (BDDE)	238:275	Regardless of various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE), seeking new strategies that enhance cross-linking efficiency with a low level of cross-linker is essential.
27312477	2	62	theme	different	498:506	arg1	approaches					515:524	two different mixing approaches	494:524	two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	494:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	7	63	theme	chemical	1445:1452	arg1	modification					1454:1465	chemical modification	1445:1465	chemical modification	1445:1465	In comparison to native HA, the occurrence of chemical modification in the cross-linked hydrogels was confirmed by FTIR and NMR distinctive peaks.
27312477	8	64	theme	higher	1609:1614	arg1	degree					1616:1621	a higher degree	1607:1621	a higher degree of modification than hydrogel 1	1607:1653	These peaks also provided evidence that hydrogel 2 exhibited a higher degree of modification than hydrogel 1.
27312477	1	65	theme	diglycidyl	253:262	arg1	BDDE					271:274	BDDE	271:274	BDDE	271:274	Regardless of various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE), seeking new strategies that enhance cross-linking efficiency with a low level of cross-linker is essential.
27312477	1	65	theme	diglycidyl	253:262	arg1	ether					264:268	1,4-butanediol diglycidyl ether	238:268	1,4-butanediol diglycidyl ether (BDDE)	238:275	Regardless of various strategies reported for cross-linking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE), seeking new strategies that enhance cross-linking efficiency with a low level of cross-linker is essential.
27312477	0	66	theme	cross-linking	42:54	arg1	efficiency					56:65	cross-linking efficiency	42:65	cross-linking efficiency of hyaluronic acid-based hydrogel	42:99	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	0	67	from	effect	13:18	arg1	efficiency					56:65	cross-linking efficiency	42:65	cross-linking efficiency of hyaluronic acid-based hydrogel	42:99	Study of the effect of mixing approach on cross-linking efficiency of hyaluronic acid-based hydrogel cross-linked with 1,4-butanediol diglycidyl ether.
27312477	7	68	theme	distinctive	1527:1537	arg1	peaks					1539:1543	distinctive peaks	1527:1543	distinctive peaks	1527:1543	In comparison to native HA, the occurrence of chemical modification in the cross-linked hydrogels was confirmed by FTIR and NMR distinctive peaks.
27312477	2	69	from	influence	415:423	arg1	hydrogels					472:480	two cross-linked BDDE-HA hydrogels	447:480	two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2)	447:853	In this work, we studied the influence of mixing approach on two cross-linked BDDE-HA hydrogels prepared by two different mixing approaches; the large-batch mixing approach in which the hydrogel quantities were all mixed as a single lump in one container (hydrogel 1), and the small-batches mixing approach in which the hydrogel quantities were divided into smaller batches, mixed separately at various HA/BDDE ratios then combined in one reaction mixture (hydrogel 2).
27312477	4	70	from	stable	1014:1019	arg1	relation					970:977	relation	970:977	relation to hydrogel 1	970:991	Degradation tests proved that, in relation to hydrogel 1, hydrogel 2 was more stable, and exhibited a higher resistance towards hyaluronidase activity.
27312477	8	71	theme	modification	1626:1637	arg1	degree					1616:1621	a higher degree	1607:1621	a higher degree of modification than hydrogel 1	1607:1653	These peaks also provided evidence that hydrogel 2 exhibited a higher degree of modification than hydrogel 1.
27312477	5	72	theme	hydrogel	1110:1117	arg1	higher					1139:1144	higher	1139:1144	higher	1139:1144	The swelling ratio of hydrogel 1 was significantly higher than that of hydrogel 2 in distilled water; however, in phosphate buffer saline, both hydrogels showed no significant difference.
27312477	5	72	theme	hydrogel	1110:1117	arg1	ratio					1101:1105	The swelling ratio	1088:1105	The swelling ratio of hydrogel 1	1088:1119	The swelling ratio of hydrogel 1 was significantly higher than that of hydrogel 2 in distilled water; however, in phosphate buffer saline, both hydrogels showed no significant difference.
25620425	5	0	theme	chemotactic	674:684	arg1	MCP-1					658:662	MCP-1	658:662	MCP-1 (monocyte chemotactic protein)	658:693	Caco spent medium enhanced the production of IL-12, IL-1Ra, IL-6, IL-8, TNF-α, MCP-1 (monocyte chemotactic protein), and MIP-1α but this was strongly attenuated by the dietary fibers.
25620425	5	0	theme	chemotactic	674:684	arg1	protein					686:692	monocyte chemotactic protein	665:692	monocyte chemotactic protein	665:692	Caco spent medium enhanced the production of IL-12, IL-1Ra, IL-6, IL-8, TNF-α, MCP-1 (monocyte chemotactic protein), and MIP-1α but this was strongly attenuated by the dietary fibers.
25620425	7	1	theme	T	1118:1118	arg1	cells					1120:1124	autologous T cells	1107:1124	autologous T cells	1107:1124	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	1	2	with	interaction	192:202	arg1	cells					261:265	dendritic cells	251:265	dendritic cells (DCs)	251:271	SCOPE In the present study, the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs) was studied.
25620425	1	2	with	interaction	192:202	arg1	DCs					268:270	DCs	268:270	DCs	268:270	SCOPE In the present study, the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs) was studied.
25620425	1	2	with	interaction	192:202	arg1	epithelial					237:246	epithelial	237:246	epithelial	237:246	SCOPE In the present study, the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs) was studied.
25620425	7	3	theme	chicory	1133:1139	arg1	inulin					1141:1146	chicory inulin	1133:1146	chicory inulin	1133:1146	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	1	4	theme	consumed	216:223	arg1	fibers					225:230	commonly consumed fibers	207:230	commonly consumed fibers	207:230	SCOPE In the present study, the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs) was studied.
25620425	0	5	from	impact	4:9	arg1	responses					47:55	dendritic cell responses	32:55	dendritic cell responses in vitro	32:64	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	9	6	theme	immune	1403:1408	arg1	system					1410:1415	the host immune system	1394:1415	the host immune system	1394:1415	CONCLUSIONS Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.
25620425	9	7	from	mechanism	1444:1452	arg1	microbiota					1468:1477	microbiota	1468:1477	microbiota	1468:1477	CONCLUSIONS Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.
25620425	7	8	with	interaction	927:937	arg1	cells					1036:1040	epithelial cells	1025:1040	epithelial cells	1025:1040	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	7	8	with	interaction	927:937	arg1	DCs					1046:1048	DCs	1046:1048	DCs	1046:1048	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	9	9	from	effects	1457:1463	arg1	microbiota					1468:1477	microbiota	1468:1477	microbiota	1468:1477	CONCLUSIONS Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.
25620425	1	10	theme	fibers	225:230	arg1	interaction					192:202	the direct interaction	181:202	the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs)	181:271	SCOPE In the present study, the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs) was studied.
25620425	4	11	from	cells	552:556	arg1	supernatants					517:528	supernatants	517:528	supernatants from human epithelial cells (Caco spent medium)	517:576	This was different when DCs and fibers were co-cultured together with supernatants from human epithelial cells (Caco spent medium).
25620425	7	12	theme	epithelial	1025:1034	arg1	cells					1036:1040	epithelial cells	1025:1040	epithelial cells	1025:1040	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	9	13	theme	effects	1457:1463	arg1	mechanism					1444:1452	the recognized mechanism	1429:1452	the recognized mechanism of effects on microbiota	1429:1477	CONCLUSIONS Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.
25620425	6	14	from	effect	780:785	arg1	cytokines					806:814	proinflammatory cytokines	790:814	proinflammatory cytokines	790:814	This attenuating effect on proinflammatory cytokines was dependent on the interaction of the fibers with Toll-like receptors as it was reduced by Pepinh-myd88.
25620425	4	15	theme	epithelial	541:550	arg1	cells					552:556	human epithelial cells	535:556	human epithelial cells (Caco spent medium)	535:576	This was different when DCs and fibers were co-cultured together with supernatants from human epithelial cells (Caco spent medium).
25620425	4	15	theme	epithelial	541:550	arg1	medium					570:575	Caco spent medium	559:575	Caco spent medium	559:575	This was different when DCs and fibers were co-cultured together with supernatants from human epithelial cells (Caco spent medium).
25620425	0	16	theme	differential	86:97	arg1	effects					99:105	the differential effects	82:105	the differential effects of the fibers on intestinal epithelial cells	82:150	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	6	17	theme	proinflammatory	790:804	arg1	cytokines					806:814	proinflammatory cytokines	790:814	proinflammatory cytokines	790:814	This attenuating effect on proinflammatory cytokines was dependent on the interaction of the fibers with Toll-like receptors as it was reduced by Pepinh-myd88.
25620425	10	18	theme	fiber	1565:1569	arg1	type					1571:1574	dietary fiber type	1557:1574	dietary fiber type	1557:1574	This modulation is dietary fiber type dependent.
25620425	7	19	theme	cytokines	1094:1102	arg1	production					1072:1081	the production	1068:1081	the production of the Th1 cytokines in autologous T cells	1068:1124	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	4	20	theme	human	535:539	arg1	cells					552:556	human epithelial cells	535:556	human epithelial cells (Caco spent medium)	535:576	This was different when DCs and fibers were co-cultured together with supernatants from human epithelial cells (Caco spent medium).
25620425	4	20	theme	human	535:539	arg1	medium					570:575	Caco spent medium	559:575	Caco spent medium	559:575	This was different when DCs and fibers were co-cultured together with supernatants from human epithelial cells (Caco spent medium).
25620425	9	21	with	interaction	1498:1508	arg1	mucosa					1530:1535	the consumer's mucosa	1515:1535	the consumer's mucosa	1515:1535	CONCLUSIONS Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.
25620425	2	22	theme	sugar	346:350	arg1	composition					352:362	their sugar composition	340:362	their sugar composition	340:362	METHODS AND RESULTS The fibers were characterized for their sugar composition and chain length profile.
25620425	5	23	theme	Caco	579:582	arg1	medium					590:595	Caco spent medium	579:595	Caco spent medium	579:595	Caco spent medium enhanced the production of IL-12, IL-1Ra, IL-6, IL-8, TNF-α, MCP-1 (monocyte chemotactic protein), and MIP-1α but this was strongly attenuated by the dietary fibers.
25620425	2	24	dep	METHODS	286:292	arg1	fibers					310:315	The fibers	306:315	METHODS AND RESULTS The fibers	286:315	METHODS AND RESULTS The fibers were characterized for their sugar composition and chain length profile.
25620425	7	25	theme	chicory	968:974	arg1	galacto-oligosaccharides					942:965	galacto-oligosaccharides	942:965	galacto-oligosaccharides	942:965	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	7	25	theme	chicory	968:974	arg1	inulin					976:981	chicory inulin	968:981	chicory inulin	968:981	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	10	26	theme	dietary	1557:1563	arg1	type					1571:1574	dietary fiber type	1557:1574	dietary fiber type	1557:1574	This modulation is dietary fiber type dependent.
25620425	7	27	from	changes	1057:1063	arg1	production					1072:1081	the production	1068:1081	the production of the Th1 cytokines in autologous T cells	1068:1124	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	5	28	theme	spent	584:588	arg1	medium					590:595	Caco spent medium	579:595	Caco spent medium	579:595	Caco spent medium enhanced the production of IL-12, IL-1Ra, IL-6, IL-8, TNF-α, MCP-1 (monocyte chemotactic protein), and MIP-1α but this was strongly attenuated by the dietary fibers.
25620425	6	29	with	interaction	837:847	arg1	receptors					878:886	Toll-like receptors	868:886	Toll-like receptors	868:886	This attenuating effect on proinflammatory cytokines was dependent on the interaction of the fibers with Toll-like receptors as it was reduced by Pepinh-myd88.
25620425	0	30	theme	dietary	14:20	arg1	fibers					22:27	dietary fibers	14:27	dietary fibers	14:27	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	1	31	theme	dendritic	251:259	arg1	cells					261:265	dendritic cells	251:265	dendritic cells (DCs)	251:271	SCOPE In the present study, the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs) was studied.
25620425	1	31	theme	dendritic	251:259	arg1	DCs					268:270	DCs	268:270	DCs	268:270	SCOPE In the present study, the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs) was studied.
25620425	7	32	theme	wheat	984:988	arg1	galacto-oligosaccharides					942:965	galacto-oligosaccharides	942:965	galacto-oligosaccharides	942:965	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	7	32	theme	wheat	984:988	arg1	arabinoxylan					990:1001	wheat arabinoxylan	984:1001	wheat arabinoxylan	984:1001	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	0	33	dep	in	57:58	arg1	vitro					60:64	vitro	60:64	vitro	60:64	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	1	34	theme	present	166:172	arg1	study					174:178	the present study	162:178	the present study	162:178	SCOPE In the present study, the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs) was studied.
25620425	8	35	theme	chicory	1282:1288	arg1	inulin					1290:1295	chicory inulin	1282:1295	chicory inulin	1282:1295	The Treg-promoting cytokine IL-10 was induced by galacto-oligosaccharides whereas chicory inulin decreased the IL-10 production.
25620425	6	36	theme	Toll-like	868:876	arg1	receptors					878:886	Toll-like receptors	868:886	Toll-like receptors	868:886	This attenuating effect on proinflammatory cytokines was dependent on the interaction of the fibers with Toll-like receptors as it was reduced by Pepinh-myd88.
25620425	6	37	theme	attenuating	768:778	arg1	effect					780:785	This attenuating effect	763:785	This attenuating effect on proinflammatory cytokines	763:814	This attenuating effect on proinflammatory cytokines was dependent on the interaction of the fibers with Toll-like receptors as it was reduced by Pepinh-myd88.
25620425	7	38	theme	galacto-oligosaccharides	942:965	arg1	interaction					927:937	The interaction	923:937	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs	923:1048	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	2	39	theme	length	374:379	arg1	profile					381:387	chain length profile	368:387	chain length profile	368:387	METHODS AND RESULTS The fibers were characterized for their sugar composition and chain length profile.
25620425	8	40	theme	cytokine	1219:1226	arg1	IL-10					1228:1232	The Treg-promoting cytokine IL-10	1200:1232	The Treg-promoting cytokine IL-10	1200:1232	The Treg-promoting cytokine IL-10 was induced by galacto-oligosaccharides whereas chicory inulin decreased the IL-10 production.
25620425	0	41	theme	fibers	114:119	arg1	effects					99:105	the differential effects	82:105	the differential effects of the fibers on intestinal epithelial cells	82:150	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	7	42	theme	Th2	1181:1183	arg1	IL-6					1194:1197	the Th2 cytokine IL-6	1177:1197	the Th2 cytokine IL-6	1177:1197	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	8	43	theme	IL-10	1311:1315	arg1	production					1317:1326	the IL-10 production	1307:1326	the IL-10 production	1307:1326	The Treg-promoting cytokine IL-10 was induced by galacto-oligosaccharides whereas chicory inulin decreased the IL-10 production.
25620425	2	44	theme	chain	368:372	arg1	profile					381:387	chain length profile	368:387	chain length profile	368:387	METHODS AND RESULTS The fibers were characterized for their sugar composition and chain length profile.
25620425	0	45	theme	fibers	22:27	arg1	impact					4:9	The impact	0:9	The impact of dietary fibers on dendritic cell responses in vitro	0:64	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	0	45	theme	fibers	22:27	arg1	dependent					69:77	dependent	69:77	dependent	69:77	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	6	46	theme	fibers	856:861	arg1	interaction					837:847	the interaction	833:847	the interaction of the fibers with Toll-like receptors	833:886	This attenuating effect on proinflammatory cytokines was dependent on the interaction of the fibers with Toll-like receptors as it was reduced by Pepinh-myd88.
25620425	7	47	from	production	1072:1081	arg1	cells					1120:1124	autologous T cells	1107:1124	autologous T cells	1107:1124	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	5	48	theme	dietary	747:753	arg1	fibers					755:760	the dietary fibers	743:760	the dietary fibers	743:760	Caco spent medium enhanced the production of IL-12, IL-1Ra, IL-6, IL-8, TNF-α, MCP-1 (monocyte chemotactic protein), and MIP-1α but this was strongly attenuated by the dietary fibers.
25620425	0	49	theme	cell	42:45	arg1	responses					47:55	dendritic cell responses	32:55	dendritic cell responses in vitro	32:64	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	9	50	theme	dietary	1366:1372	arg1	fibers					1374:1379	dietary fibers	1366:1379	dietary fibers	1366:1379	CONCLUSIONS Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.
25620425	0	51	theme	intestinal	124:133	arg1	cells					146:150	intestinal epithelial cells	124:150	intestinal epithelial cells	124:150	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	9	52	theme	host	1398:1401	arg1	system					1410:1415	the host immune system	1394:1415	the host immune system	1394:1415	CONCLUSIONS Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.
25620425	0	53	theme	dendritic	32:40	arg1	responses					47:55	dendritic cell responses	32:55	dendritic cell responses in vitro	32:64	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	5	54	theme	IL-12	624:628	arg1	production					610:619	the production	606:619	the production of IL-12, IL-1Ra, IL-6, IL-8, TNF-α, MCP-1 (monocyte chemotactic protein), and MIP-1α	606:705	Caco spent medium enhanced the production of IL-12, IL-1Ra, IL-6, IL-8, TNF-α, MCP-1 (monocyte chemotactic protein), and MIP-1α but this was strongly attenuated by the dietary fibers.
25620425	1	55	dep	SCOPE	153:157	arg1	study					174:178	the present study	162:178	the present study	162:178	SCOPE In the present study, the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs) was studied.
25620425	7	56	theme	autologous	1107:1116	arg1	cells					1120:1124	autologous T cells	1107:1124	autologous T cells	1107:1124	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	7	57	theme	barley	1153:1158	arg1	β-glucan					1160:1167	barley β-glucan	1153:1167	barley β-glucan	1153:1167	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	9	58	theme	direct	1491:1496	arg1	interaction					1498:1508	direct interaction	1491:1508	direct interaction with the consumer's mucosa	1491:1535	CONCLUSIONS Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.
25620425	1	59	theme	direct	185:190	arg1	interaction					192:202	the direct interaction	181:202	the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs)	181:271	SCOPE In the present study, the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DCs) was studied.
25620425	0	60	theme	epithelial	135:144	arg1	cells					146:150	intestinal epithelial cells	124:150	intestinal epithelial cells	124:150	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	9	61	dep	CONCLUSIONS	1329:1339	arg1	suggest					1353:1359	suggest	1353:1359	suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.	1353:1536	CONCLUSIONS Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.
25620425	9	62	theme	recognized	1433:1442	arg1	mechanism					1444:1452	the recognized mechanism	1429:1452	the recognized mechanism of effects on microbiota	1429:1477	CONCLUSIONS Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.
25620425	7	63	theme	barley	1004:1009	arg1	β-glucan					1011:1018	barley β-glucan	1004:1018	barley β-glucan	1004:1018	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	7	63	theme	barley	1004:1009	arg1	galacto-oligosaccharides					942:965	galacto-oligosaccharides	942:965	galacto-oligosaccharides	942:965	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	0	64	theme	in	57:58	arg1	responses					47:55	dendritic cell responses	32:55	dendritic cell responses in vitro	32:64	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25620425	7	65	theme	cytokine	1185:1192	arg1	IL-6					1194:1197	the Th2 cytokine IL-6	1177:1197	the Th2 cytokine IL-6	1177:1197	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	8	66	theme	Treg-promoting	1204:1217	arg1	IL-10					1228:1232	The Treg-promoting cytokine IL-10	1200:1232	The Treg-promoting cytokine IL-10	1200:1232	The Treg-promoting cytokine IL-10 was induced by galacto-oligosaccharides whereas chicory inulin decreased the IL-10 production.
25620425	3	67	theme	direct	398:403	arg1	contact					405:411	direct contact	398:411	direct contact	398:411	When in direct contact, fibers activate DCs only mildly.
25620425	5	68	theme	monocyte	665:672	arg1	MCP-1					658:662	MCP-1	658:662	MCP-1 (monocyte chemotactic protein)	658:693	Caco spent medium enhanced the production of IL-12, IL-1Ra, IL-6, IL-8, TNF-α, MCP-1 (monocyte chemotactic protein), and MIP-1α but this was strongly attenuated by the dietary fibers.
25620425	5	68	theme	monocyte	665:672	arg1	protein					686:692	monocyte chemotactic protein	665:692	monocyte chemotactic protein	665:692	Caco spent medium enhanced the production of IL-12, IL-1Ra, IL-6, IL-8, TNF-α, MCP-1 (monocyte chemotactic protein), and MIP-1α but this was strongly attenuated by the dietary fibers.
25620425	7	69	theme	Th1	1090:1092	arg1	cytokines					1094:1102	the Th1 cytokines	1086:1102	the Th1 cytokines	1086:1102	The interaction of galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokine IL-6.
25620425	4	70	theme	spent	564:568	arg1	cells					552:556	human epithelial cells	535:556	human epithelial cells (Caco spent medium)	535:576	This was different when DCs and fibers were co-cultured together with supernatants from human epithelial cells (Caco spent medium).
25620425	4	70	theme	spent	564:568	arg1	medium					570:575	Caco spent medium	559:575	Caco spent medium	559:575	This was different when DCs and fibers were co-cultured together with supernatants from human epithelial cells (Caco spent medium).
25620425	4	71	theme	Caco	559:562	arg1	cells					552:556	human epithelial cells	535:556	human epithelial cells (Caco spent medium)	535:576	This was different when DCs and fibers were co-cultured together with supernatants from human epithelial cells (Caco spent medium).
25620425	4	71	theme	Caco	559:562	arg1	medium					570:575	Caco spent medium	559:575	Caco spent medium	559:575	This was different when DCs and fibers were co-cultured together with supernatants from human epithelial cells (Caco spent medium).
25620425	9	72	from	microbiota	1468:1477	arg1	mechanism					1444:1452	the recognized mechanism	1429:1452	the recognized mechanism of effects on microbiota	1429:1477	CONCLUSIONS Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer's mucosa.
25620425	0	73	from	effects	99:105	arg1	cells					146:150	intestinal epithelial cells	124:150	intestinal epithelial cells	124:150	The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells.
25308166	8	0	dep	variation	1446:1454	arg1	3					1443:1443	3	1443:1443	3	1443:1443	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	1	1	theme	chemical	143:150	arg1	compositions					152:163	nearly identical chemical compositions	126:163	nearly identical chemical compositions	126:163	Although beet and cane sugar sources have nearly identical chemical compositions, the sugars differ in their volatile profiles, thermal behaviors, and minor chemical components.
25308166	4	2	from	sugars	669:674	arg1	regard					679:684	regard	679:684	regard to their aroma and flavor	679:710	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	0	3	theme	sugar	69:73	arg1	sources					75:81	cane sugar sources	64:81	cane sugar sources	64:81	Sensory differences between product matrices made with beet and cane sugar sources.
25308166	8	4	from	quantity	1401:1408	arg1	products					1428:1435	these products	1422:1435	these products	1422:1435	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	3	5	theme	cane	506:509	arg1	sources					517:523	cane sugar sources	506:523	cane sugar sources	506:523	The objective of this research was to determine whether panelists could identify a sensory difference between product matrices made with beet and cane sugar sources.
25308166	8	6	from	difference	1237:1246	arg1	products					1257:1264	these products	1251:1264	these products	1251:1264	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	1	7	theme	minor	235:239	arg1	components					250:259	minor chemical components	235:259	minor chemical components	235:259	Although beet and cane sugar sources have nearly identical chemical compositions, the sugars differ in their volatile profiles, thermal behaviors, and minor chemical components.
25308166	8	8	from	flavor	1331:1336	arg1	products					1428:1435	these products	1422:1435	these products	1422:1435	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	7	9	located	observed	1130:1137	arg1	tea					1194:1196	iced tea	1189:1196	iced tea	1189:1196	No difference was observed in the sugar cookies, pudding, whipped cream, and iced tea.
25308166	7	9	located	observed	1130:1137	arg2	difference					1115:1124	No difference	1112:1124	No difference	1112:1124	No difference was observed in the sugar cookies, pudding, whipped cream, and iced tea.
25308166	7	9	located	observed	1130:1137	arg1	pudding					1161:1167	pudding	1161:1167	pudding	1161:1167	No difference was observed in the sugar cookies, pudding, whipped cream, and iced tea.
25308166	7	9	located	observed	1130:1137	arg1	cream					1178:1182	whipped cream	1170:1182	whipped cream	1170:1182	No difference was observed in the sugar cookies, pudding, whipped cream, and iced tea.
25308166	7	9	located	observed	1130:1137	arg1	cookies					1152:1158	the sugar cookies	1142:1158	the sugar cookies	1142:1158	No difference was observed in the sugar cookies, pudding, whipped cream, and iced tea.
25308166	4	10	from	difference	726:735	arg1	pudding					778:784	pudding	778:784	pudding	778:784	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	4	10	from	difference	726:735	arg1	syrup					756:760	simple syrup	749:760	simple syrup	749:760	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	4	10	from	difference	726:735	arg1	cream					795:799	whipped cream	787:799	whipped cream	787:799	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	4	10	from	difference	726:735	arg1	cookies					769:775	sugar cookies	763:775	sugar cookies	763:775	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	4	10	from	difference	726:735	arg1	pavlova					740:746	pavlova	740:746	pavlova	740:746	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	4	10	from	difference	726:735	arg1	tea					811:813	iced tea	806:813	iced tea	806:813	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	5	11	theme	rank	877:880	arg1	tests					886:890	Friedman's rank sum tests	866:890	Friedman's rank sum tests	866:890	R-index values and Friedman's rank sum tests showed differences (P < 0.05) between beet and cane sugars in regard to their aroma and flavor.
25308166	1	12	theme	chemical	241:248	arg1	components					250:259	minor chemical components	235:259	minor chemical components	235:259	Although beet and cane sugar sources have nearly identical chemical compositions, the sugars differ in their volatile profiles, thermal behaviors, and minor chemical components.
25308166	8	13	theme	matrix	1368:1373	arg1	quantity					1401:1408	the relatively small quantity	1380:1408	(2) the relatively small quantity of sugar in these products	1376:1435	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	8	13	theme	matrix	1368:1373	arg1	variation					1446:1454	(3) variation	1442:1454	(3) variation	1442:1454	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	8	13	theme	matrix	1368:1373	arg1	complexity					1342:1351	complexity	1342:1351	complexity	1342:1351	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	8	13	theme	matrix	1368:1373	arg1	flavor					1331:1336	the flavor	1327:1336	the flavor	1327:1336	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	4	14	theme	iced	806:809	arg1	tea					811:813	iced tea	806:813	iced tea	806:813	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	5	15	theme	sum	882:884	arg1	tests					886:890	Friedman's rank sum tests	866:890	Friedman's rank sum tests	866:890	R-index values and Friedman's rank sum tests showed differences (P < 0.05) between beet and cane sugars in regard to their aroma and flavor.
25308166	4	16	from	brands	654:659	arg1	regard					679:684	regard	679:684	regard to their aroma and flavor	679:710	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	5	17	theme	R-index	847:853	arg1	values					855:860	R-index values	847:860	R-index values	847:860	R-index values and Friedman's rank sum tests showed differences (P < 0.05) between beet and cane sugars in regard to their aroma and flavor.
25308166	6	18	theme	sugar	1024:1028	arg1	sources					1030:1036	the sugar sources	1020:1036	the sugar sources	1020:1036	Significant differences between the sugar sources were also identified when incorporated into the pavlova and simple syrup.
25308166	8	19	dep	quantity	1401:1408	arg1	2					1377:1377	2	1377:1377	2	1377:1377	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	9	20	theme	cane	1677:1680	arg1	sugars					1682:1687	beet and cane sugars	1668:1687	sugars	1682:1687	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	2	21	theme	Scientific	262:271	arg1	evidence					273:280	Scientific evidence	262:280	Scientific evidence characterizing the impact of these differences on product quality	262:346	Scientific evidence characterizing the impact of these differences on product quality is lacking.
25308166	8	22	theme	Possible	1199:1206	arg1	explanations					1208:1219	Possible explanations	1199:1219	Possible explanations for the lack of difference in these products	1199:1264	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	9	23	from	matrices	1701:1708	arg1	interchangeable					1757:1771	interchangeable	1757:1771	interchangeable	1757:1771	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	4	24	used	used	546:549	arg2	panelists					536:544	Sixty-two panelists	526:544	Sixty-two panelists	526:544	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	0	25	theme	Sensory	0:6	arg1	differences					8:18	Sensory differences	0:18	Sensory differences between product matrices	0:43	Sensory differences between product matrices made with beet and cane sugar sources.
25308166	5	26	from	differences	899:909	arg1	regard					954:959	regard	954:959	regard to their aroma and flavor	954:985	R-index values and Friedman's rank sum tests showed differences (P < 0.05) between beet and cane sugars in regard to their aroma and flavor.
25308166	4	27	theme	beet	643:646	arg1	brands					633:638	2 brands	631:638	2 brands of beet	631:646	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	4	27	theme	beet	643:646	arg1	brands					654:659	2 brands	652:659	2 brands of cane sugars in regard to their aroma and flavor	652:710	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	9	28	theme	sugar	1572:1576	arg1	manufacturers					1578:1590	sugar manufacturers	1572:1590	sugar manufacturers	1572:1590	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	7	29	theme	whipped	1170:1176	arg1	cream					1178:1182	whipped cream	1170:1182	whipped cream	1170:1182	No difference was observed in the sugar cookies, pudding, whipped cream, and iced tea.
25308166	7	30	theme	sugar	1146:1150	arg1	cookies					1152:1158	the sugar cookies	1142:1158	the sugar cookies	1142:1158	No difference was observed in the sugar cookies, pudding, whipped cream, and iced tea.
25308166	8	31	theme	difference	1237:1246	arg1	lack					1229:1232	the lack	1225:1232	the lack of difference in these products	1225:1264	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	8	32	theme	product	1360:1366	arg1	matrix					1368:1373	the product matrix	1356:1373	the product matrix	1356:1373	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	7	33	theme	iced	1189:1192	arg1	tea					1194:1196	iced tea	1189:1196	iced tea	1189:1196	No difference was observed in the sugar cookies, pudding, whipped cream, and iced tea.
25308166	3	34	theme	research	382:389	arg1	objective					364:372	The objective	360:372	The objective of this research	360:389	The objective of this research was to determine whether panelists could identify a sensory difference between product matrices made with beet and cane sugar sources.
25308166	9	35	from	interchangeable	1757:1771	arg1	sugars					1682:1687	beet and cane sugars	1668:1687	sugars	1682:1687	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	9	35	from	interchangeable	1757:1771	arg1	matrices					1701:1708	product matrices	1693:1708	product matrices	1693:1708	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	4	36	theme	whipped	787:793	arg1	cream					795:799	whipped cream	787:799	whipped cream	787:799	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	8	37	dep	masking	1279:1285	arg1	1					1276:1276	1	1276:1276	1	1276:1276	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	9	38	theme	beet	1719:1722	arg1	sugars					1733:1738	beet and cane sugars	1719:1738	sugars	1733:1738	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	8	39	theme	beet	1290:1293	arg1	differences					1312:1322	beet and cane sensory differences	1290:1322	differences	1312:1322	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	0	40	theme	product	28:34	arg1	matrices					36:43	product matrices	28:43	product matrices	28:43	Sensory differences between product matrices made with beet and cane sugar sources.
25308166	1	41	theme	beet	93:96	arg1	sources					113:119	beet and cane sugar sources	93:119	sources	113:119	Although beet and cane sugar sources have nearly identical chemical compositions, the sugars differ in their volatile profiles, thermal behaviors, and minor chemical components.
25308166	4	42	from	brands	633:638	arg1	regard					679:684	regard	679:684	regard to their aroma and flavor	679:710	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	4	43	theme	cane	834:837	arg1	sugars					839:844	cane sugars	834:844	cane sugars	834:844	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	4	44	theme	sugars	669:674	arg1	brands					633:638	2 brands	631:638	2 brands of beet	631:646	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	4	44	theme	sugars	669:674	arg1	brands					654:659	2 brands	652:659	2 brands of cane sugars in regard to their aroma and flavor	652:710	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	9	45	theme	cane	1728:1731	arg1	sugars					1733:1738	beet and cane sugars	1719:1738	sugars	1733:1738	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	3	46	theme	product	470:476	arg1	matrices					478:485	product matrices	470:485	product matrices made with beet and cane sugar sources	470:523	The objective of this research was to determine whether panelists could identify a sensory difference between product matrices made with beet and cane sugar sources.
25308166	2	47	theme	product	332:338	arg1	quality					340:346	product quality	332:346	product quality	332:346	Scientific evidence characterizing the impact of these differences on product quality is lacking.
25308166	3	48	theme	sensory	443:449	arg1	difference					451:460	a sensory difference	441:460	a sensory difference between product matrices made with beet and cane sugar sources	441:523	The objective of this research was to determine whether panelists could identify a sensory difference between product matrices made with beet and cane sugar sources.
25308166	4	49	theme	sugar	763:767	arg1	cookies					769:775	sugar cookies	763:775	sugar cookies	763:775	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	9	50	theme	beet	1668:1671	arg1	sugars					1682:1687	beet and cane sugars	1668:1687	sugars	1682:1687	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	1	51	theme	volatile	193:200	arg1	profiles					202:209	their volatile profiles	187:209	their volatile profiles	187:209	Although beet and cane sugar sources have nearly identical chemical compositions, the sugars differ in their volatile profiles, thermal behaviors, and minor chemical components.
25308166	4	52	theme	cane	664:667	arg1	sugars					669:674	cane sugars	664:674	cane sugars in regard to their aroma and flavor	664:710	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	8	53	theme	sensory	1304:1310	arg1	differences					1312:1322	beet and cane sensory differences	1290:1322	differences	1312:1322	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	8	54	from	complexity	1342:1351	arg1	products					1428:1435	these products	1422:1435	these products	1422:1435	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	9	55	from	sugars	1682:1687	arg1	interchangeable					1757:1771	interchangeable	1757:1771	interchangeable	1757:1771	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	8	56	theme	small	1395:1399	arg1	quantity					1401:1408	the relatively small quantity	1380:1408	(2) the relatively small quantity of sugar in these products	1376:1435	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	8	57	theme	cane	1299:1302	arg1	differences					1312:1322	beet and cane sensory differences	1290:1322	differences	1312:1322	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	6	58	theme	Significant	988:998	arg1	differences					1000:1010	Significant differences	988:1010	Significant differences between the sugar sources	988:1036	Significant differences between the sugar sources were also identified when incorporated into the pavlova and simple syrup.
25308166	1	59	theme	cane	102:105	arg1	sources					113:119	beet and cane sugar sources	93:119	sources	113:119	Although beet and cane sugar sources have nearly identical chemical compositions, the sugars differ in their volatile profiles, thermal behaviors, and minor chemical components.
25308166	8	60	theme	sugar	1510:1514	arg1	source					1516:1521	the sugar source	1506:1521	the sugar source	1506:1521	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	8	61	theme	sugar	1413:1417	arg1	quantity					1401:1408	the relatively small quantity	1380:1408	(2) the relatively small quantity of sugar in these products	1376:1435	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	8	61	theme	sugar	1413:1417	arg1	variation					1446:1454	(3) variation	1442:1454	(3) variation	1442:1454	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	8	61	theme	sugar	1413:1417	arg1	complexity					1342:1351	complexity	1342:1351	complexity	1342:1351	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	8	61	theme	sugar	1413:1417	arg1	flavor					1331:1336	the flavor	1327:1336	the flavor	1327:1336	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	5	62	theme	beet	930:933	arg1	sugars					944:949	beet and cane sugars	930:949	sugars	944:949	R-index values and Friedman's rank sum tests showed differences (P < 0.05) between beet and cane sugars in regard to their aroma and flavor.
25308166	1	63	theme	sugar	107:111	arg1	sources					113:119	beet and cane sugar sources	93:119	sources	113:119	Although beet and cane sugar sources have nearly identical chemical compositions, the sugars differ in their volatile profiles, thermal behaviors, and minor chemical components.
25308166	8	64	from	lack	1229:1232	arg1	products					1257:1264	these products	1251:1264	these products	1251:1264	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	6	65	theme	pavlova	1086:1092	arg1	syrup					1105:1109	the pavlova and simple syrup	1082:1109	the pavlova and simple syrup	1082:1109	Significant differences between the sugar sources were also identified when incorporated into the pavlova and simple syrup.
25308166	8	66	theme	differences	1312:1322	arg1	masking					1279:1285	(1) masking	1275:1285	(1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation	1275:1454	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	5	67	theme	cane	939:942	arg1	sugars					944:949	beet and cane sugars	930:949	sugars	944:949	R-index values and Friedman's rank sum tests showed differences (P < 0.05) between beet and cane sugars in regard to their aroma and flavor.
25308166	8	68	from	products	1257:1264	arg1	lack					1229:1232	the lack	1225:1232	the lack of difference in these products	1225:1264	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	8	69	from	variation	1446:1454	arg1	products					1428:1435	these products	1422:1435	these products	1422:1435	Possible explanations for the lack of difference in these products include: (1) masking of beet and cane sensory differences by the flavor and complexity of the product matrix, (2) the relatively small quantity of sugar in these products, and (3) variation within these products being more influential than the sugar source.
25308166	9	70	from	research	1547:1554	arg1	relevant					1560:1567	relevant	1560:1567	relevant	1560:1567	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	9	70	from	research	1547:1554	arg1	findings					1528:1535	The findings	1524:1535	The findings from this research	1524:1554	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	6	71	theme	simple	1098:1103	arg1	syrup					1105:1109	the pavlova and simple syrup	1082:1109	the pavlova and simple syrup	1082:1109	Significant differences between the sugar sources were also identified when incorporated into the pavlova and simple syrup.
25308166	2	72	theme	differences	317:327	arg1	impact					301:306	the impact	297:306	the impact of these differences on product quality	297:346	Scientific evidence characterizing the impact of these differences on product quality is lacking.
25308166	9	73	theme	food	1600:1603	arg1	industry					1605:1612	the food industry	1596:1612	the food industry	1596:1612	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	1	74	theme	thermal	212:218	arg1	behaviors					220:228	thermal behaviors	212:228	thermal behaviors	212:228	Although beet and cane sugar sources have nearly identical chemical compositions, the sugars differ in their volatile profiles, thermal behaviors, and minor chemical components.
25308166	0	75	theme	cane	64:67	arg1	sources					75:81	cane sugar sources	64:81	cane sugar sources	64:81	Sensory differences between product matrices made with beet and cane sugar sources.
25308166	9	76	theme	product	1693:1699	arg1	matrices					1701:1708	product matrices	1693:1708	product matrices	1693:1708	The findings from this research are relevant to sugar manufacturers and the food industry as a whole, because it identifies differences between beet and cane sugars and product matrices in which beet and cane sugars are not directly interchangeable.
25308166	5	77	theme	P	912:912	arg1	differences					899:909	differences	899:909	differences (P < 0.05) between beet and cane sugars in regard to their aroma and flavor	899:985	R-index values and Friedman's rank sum tests showed differences (P < 0.05) between beet and cane sugars in regard to their aroma and flavor.
25308166	5	77	theme	P	912:912	arg1	<					914:914	P < 0.05	912:919	P < 0.05	912:919	R-index values and Friedman's rank sum tests showed differences (P < 0.05) between beet and cane sugars in regard to their aroma and flavor.
25308166	3	78	theme	sugar	511:515	arg1	sources					517:523	cane sugar sources	506:523	cane sugar sources	506:523	The objective of this research was to determine whether panelists could identify a sensory difference between product matrices made with beet and cane sugar sources.
25308166	4	79	from	regard	679:684	arg1	brands					633:638	2 brands	631:638	2 brands of beet	631:646	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	4	79	from	regard	679:684	arg1	brands					654:659	2 brands	652:659	2 brands of cane sugars in regard to their aroma and flavor	652:710	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	1	80	contain	have	121:124	arg1	sources					113:119	beet and cane sugar sources	93:119	sources	113:119	Although beet and cane sugar sources have nearly identical chemical compositions, the sugars differ in their volatile profiles, thermal behaviors, and minor chemical components.
25308166	1	80	contain	have	121:124	arg2	compositions					152:163	nearly identical chemical compositions	126:163	nearly identical chemical compositions	126:163	Although beet and cane sugar sources have nearly identical chemical compositions, the sugars differ in their volatile profiles, thermal behaviors, and minor chemical components.
25308166	4	81	theme	simple	749:754	arg1	syrup					756:760	simple syrup	749:760	simple syrup	749:760	Sixty-two panelists used the R-index by ranking method to discern whether there was a difference between 2 brands of beet and 2 brands of cane sugars in regard to their aroma and flavor, along with a difference in pavlova, simple syrup, sugar cookies, pudding, whipped cream, and iced tea made with beet and cane sugars.
25308166	1	82	theme	identical	133:141	arg1	compositions					152:163	nearly identical chemical compositions	126:163	nearly identical chemical compositions	126:163	Although beet and cane sugar sources have nearly identical chemical compositions, the sugars differ in their volatile profiles, thermal behaviors, and minor chemical components.
25308166	2	83	from	impact	301:306	arg1	quality					340:346	product quality	332:346	product quality	332:346	Scientific evidence characterizing the impact of these differences on product quality is lacking.
23789772	1	0	theme	chorda	170:175	arg1	Rhodophyta					193:202	Rhodophyta	193:202	Rhodophyta	193:202	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	0	theme	chorda	170:175	arg1	Gracilariopsis					155:168	Gracilariopsis chorda	155:175	Gracilariopsis chorda (Gracilariales, Rhodophyta)	155:203	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	1	dep	Abstract	96:103	arg1	sequenced					108:116	sequenced	108:116	sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific	108:295	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	2	2	from	bp	323:324	arg1	length					329:334	length	329:334	length	329:334	The mitogenome is 26,534 bp in length with 27.7% GC content that consists of 52 genes including 26 protein-coding, 2 rRNA and 24 tRNA genes.
23789772	2	3	theme	%	345:345	arg1	content					350:356	27.7% GC content	341:356	27.7% GC content that consists of 52 genes including 26 protein-coding, 2 rRNA and 24 tRNA genes	341:436	The mitogenome is 26,534 bp in length with 27.7% GC content that consists of 52 genes including 26 protein-coding, 2 rRNA and 24 tRNA genes.
23789772	2	4	with	bp	323:324	arg1	content					350:356	27.7% GC content	341:356	27.7% GC content that consists of 52 genes including 26 protein-coding, 2 rRNA and 24 tRNA genes	341:436	The mitogenome is 26,534 bp in length with 27.7% GC content that consists of 52 genes including 26 protein-coding, 2 rRNA and 24 tRNA genes.
23789772	2	5	theme	GC	347:348	arg1	content					350:356	27.7% GC content	341:356	27.7% GC content that consists of 52 genes including 26 protein-coding, 2 rRNA and 24 tRNA genes	341:436	The mitogenome is 26,534 bp in length with 27.7% GC content that consists of 52 genes including 26 protein-coding, 2 rRNA and 24 tRNA genes.
23789772	3	6	theme	chorda	454:459	arg1	mitogenome					461:470	G. chorda mitogenome	451:470	G. chorda mitogenome	451:470	We compared G. chorda mitogenome with that of recently published G. lemaneiformis.
23789772	3	7	theme	published	494:502	arg1	lemaneiformis					507:519	recently published G. lemaneiformis	485:519	recently published G. lemaneiformis	485:519	We compared G. chorda mitogenome with that of recently published G. lemaneiformis.
23789772	2	8	theme	tRNA	427:430	arg1	genes					432:436	26 protein-coding, 2 rRNA and 24 tRNA genes	394:436	genes	432:436	The mitogenome is 26,534 bp in length with 27.7% GC content that consists of 52 genes including 26 protein-coding, 2 rRNA and 24 tRNA genes.
23789772	3	9	theme	G.	504:505	arg1	lemaneiformis					507:519	recently published G. lemaneiformis	485:519	recently published G. lemaneiformis	485:519	We compared G. chorda mitogenome with that of recently published G. lemaneiformis.
23789772	0	10	theme	mitochondrial	9:21	arg1	genome					23:28	Complete mitochondrial genome	0:28	Complete mitochondrial genome of agar-producing red alga Gracilariopsis	0:70	Complete mitochondrial genome of agar-producing red alga Gracilariopsis chorda (Gracilariales).
23789772	4	11	theme	sequence	533:540	arg1	similarity					542:551	Nucleotide sequence similarity	522:551	Nucleotide sequence similarity between these two mitogenomes	522:581	Nucleotide sequence similarity between these two mitogenomes was 99.82%, however, there was significant difference in length caused by trnR trnS and trnY genes missing in G. lemaneiformis.
23789772	0	12	theme	Complete	0:7	arg1	genome					23:28	Complete mitochondrial genome	0:28	Complete mitochondrial genome of agar-producing red alga Gracilariopsis	0:70	Complete mitochondrial genome of agar-producing red alga Gracilariopsis chorda (Gracilariales).
23789772	1	13	theme	northwest	279:287	arg1	Pacific					289:295	the northwest Pacific	275:295	the northwest Pacific	275:295	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	4	14	theme	Nucleotide	522:531	arg1	similarity					542:551	Nucleotide sequence similarity	522:551	Nucleotide sequence similarity between these two mitogenomes	522:581	Nucleotide sequence similarity between these two mitogenomes was 99.82%, however, there was significant difference in length caused by trnR trnS and trnY genes missing in G. lemaneiformis.
23789772	0	15	dep	chorda	72:77	arg1	Gracilariales					80:92	Gracilariales	80:92	Gracilariales	80:92	Complete mitochondrial genome of agar-producing red alga Gracilariopsis chorda (Gracilariales).
23789772	2	16	theme	protein-coding	397:410	arg1	rRNA					415:418	26 protein-coding, 2 rRNA and 24 tRNA genes	394:436	rRNA	415:418	The mitogenome is 26,534 bp in length with 27.7% GC content that consists of 52 genes including 26 protein-coding, 2 rRNA and 24 tRNA genes.
23789772	0	17	theme	red	48:50	arg1	Gracilariopsis					57:70	agar-producing red alga Gracilariopsis	33:70	agar-producing red alga Gracilariopsis	33:70	Complete mitochondrial genome of agar-producing red alga Gracilariopsis chorda (Gracilariales).
23789772	4	18	theme	trnY	671:674	arg1	genes					676:680	trnY genes	671:680	trnY genes	671:680	Nucleotide sequence similarity between these two mitogenomes was 99.82%, however, there was significant difference in length caused by trnR trnS and trnY genes missing in G. lemaneiformis.
23789772	4	19	theme	significant	614:624	arg1	difference					626:635	significant difference	614:635	significant difference	614:635	Nucleotide sequence similarity between these two mitogenomes was 99.82%, however, there was significant difference in length caused by trnR trnS and trnY genes missing in G. lemaneiformis.
23789772	0	20	theme	agar-producing	33:46	arg1	Gracilariopsis					57:70	agar-producing red alga Gracilariopsis	33:70	agar-producing red alga Gracilariopsis	33:70	Complete mitochondrial genome of agar-producing red alga Gracilariopsis chorda (Gracilariales).
23789772	0	21	theme	Gracilariopsis	57:70	arg1	genome					23:28	Complete mitochondrial genome	0:28	Complete mitochondrial genome of agar-producing red alga Gracilariopsis	0:70	Complete mitochondrial genome of agar-producing red alga Gracilariopsis chorda (Gracilariales).
23789772	2	22	theme	27.7	341:344	arg1	%					345:345	%	345:345	%	345:345	The mitogenome is 26,534 bp in length with 27.7% GC content that consists of 52 genes including 26 protein-coding, 2 rRNA and 24 tRNA genes.
23789772	0	23	theme	alga	52:55	arg1	Gracilariopsis					57:70	agar-producing red alga Gracilariopsis	33:70	agar-producing red alga Gracilariopsis	33:70	Complete mitochondrial genome of agar-producing red alga Gracilariopsis chorda (Gracilariales).
23789772	1	24	theme	complete	122:129	arg1	genome					145:150	the complete mitochondrial genome	118:150	the complete mitochondrial genome	118:150	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	24	theme	complete	122:129	arg1	species					252:258	an agar-producing economic red algal species	215:258	an agar-producing economic red algal species distributed in the northwest Pacific	215:295	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	25	theme	mitochondrial	131:143	arg1	genome					145:150	the complete mitochondrial genome	118:150	the complete mitochondrial genome	118:150	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	25	theme	mitochondrial	131:143	arg1	species					252:258	an agar-producing economic red algal species	215:258	an agar-producing economic red algal species distributed in the northwest Pacific	215:295	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	26	theme	agar-producing	218:231	arg1	genome					145:150	the complete mitochondrial genome	118:150	the complete mitochondrial genome	118:150	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	26	theme	agar-producing	218:231	arg1	species					252:258	an agar-producing economic red algal species	215:258	an agar-producing economic red algal species distributed in the northwest Pacific	215:295	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	3	27	theme	G.	451:452	arg1	mitogenome					461:470	G. chorda mitogenome	451:470	G. chorda mitogenome	451:470	We compared G. chorda mitogenome with that of recently published G. lemaneiformis.
23789772	1	28	theme	economic	233:240	arg1	genome					145:150	the complete mitochondrial genome	118:150	the complete mitochondrial genome	118:150	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	28	theme	economic	233:240	arg1	species					252:258	an agar-producing economic red algal species	215:258	an agar-producing economic red algal species distributed in the northwest Pacific	215:295	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	4	29	theme	trnR	657:660	arg1	trnS					662:665	trnR trnS	657:665	trnR trnS	657:665	Nucleotide sequence similarity between these two mitogenomes was 99.82%, however, there was significant difference in length caused by trnR trnS and trnY genes missing in G. lemaneiformis.
23789772	1	30	theme	red	242:244	arg1	genome					145:150	the complete mitochondrial genome	118:150	the complete mitochondrial genome	118:150	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	30	theme	red	242:244	arg1	species					252:258	an agar-producing economic red algal species	215:258	an agar-producing economic red algal species distributed in the northwest Pacific	215:295	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	31	theme	Gracilariopsis	155:168	arg1	genome					145:150	the complete mitochondrial genome	118:150	the complete mitochondrial genome	118:150	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	31	theme	Gracilariopsis	155:168	arg1	species					252:258	an agar-producing economic red algal species	215:258	an agar-producing economic red algal species distributed in the northwest Pacific	215:295	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	32	theme	algal	246:250	arg1	genome					145:150	the complete mitochondrial genome	118:150	the complete mitochondrial genome	118:150	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
23789772	1	32	theme	algal	246:250	arg1	species					252:258	an agar-producing economic red algal species	215:258	an agar-producing economic red algal species distributed in the northwest Pacific	215:295	Abstract We sequenced the complete mitochondrial genome of Gracilariopsis chorda (Gracilariales, Rhodophyta), which is an agar-producing economic red algal species distributed in the northwest Pacific.
25148261	4	0	theme	reduction	876:884	arg1	PAGE					890:893	delayed reduction SDS PAGE	868:893	delayed reduction SDS PAGE	868:893	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	7	1	theme	higher	1261:1266	arg1	values					1305:1310	significantly higher total collagen and glycosaminoglycan values	1247:1310	significantly higher total collagen and glycosaminoglycan values (p<0.05)	1247:1319	Ovine vaginal tissue showed significantly higher total collagen and glycosaminoglycan values (p<0.05) nearest the cervix.
25148261	7	1	theme	higher	1261:1266	arg1	p<0.05					1313:1318	p<0.05	1313:1318	p<0.05	1313:1318	Ovine vaginal tissue showed significantly higher total collagen and glycosaminoglycan values (p<0.05) nearest the cervix.
25148261	11	2	theme	variation	1855:1863	arg1	Knowledge					1828:1836	Knowledge	1828:1836	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall	1828:1928	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall will assist future studies using sheep as a model for vaginal surgery.
25148261	6	3	theme	comparable	1188:1197	arg1	composition					1206:1216	comparable tissue composition	1188:1216	comparable tissue composition	1188:1216	RESULTS Both sheep and human vaginal tissue showed comparable tissue composition.
25148261	3	4	theme	DESIGN	565:570	arg1	tissue					580:585	STUDY DESIGN Vaginal tissue	559:585	STUDY DESIGN Vaginal tissue	559:585	STUDY DESIGN Vaginal tissue was collected from ovariectomised sheep (n = 6) and from postmenopausal women (n = 7) from the proximal, middle and distal thirds.
25148261	11	5	theme	vaginal	1984:1990	arg1	surgery					1992:1998	vaginal surgery	1984:1998	vaginal surgery	1984:1998	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall will assist future studies using sheep as a model for vaginal surgery.
25148261	4	6	theme	III/I+III	848:856	arg1	ratios					858:863	collagen III/I+III ratios	839:863	collagen III/I+III ratios by delayed reduction SDS PAGE	839:893	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	10	7	theme	vaginal	1729:1735	arg1	wall					1737:1740	the postmenopausal vaginal wall	1710:1740	the postmenopausal vaginal wall	1710:1740	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	1	8	theme	tissue	187:192	arg1	composition					194:204	human vaginal tissue composition	173:204	human vaginal tissue composition	173:204	OBJECTIVE There are increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results.
25148261	2	9	from	tissue	351:356	arg1	terms					361:365	terms	361:365	terms of histological and biochemical tissue composition	361:416	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	6	10	theme	human	1160:1164	arg1	tissue					1174:1179	human vaginal tissue	1160:1179	human vaginal tissue	1160:1179	RESULTS Both sheep and human vaginal tissue showed comparable tissue composition.
25148261	6	10	theme	human	1160:1164	arg1	RESULTS					1137:1143	RESULTS Both sheep and human vaginal tissue	1137:1179	RESULTS Both sheep and human vaginal tissue	1137:1179	RESULTS Both sheep and human vaginal tissue showed comparable tissue composition.
25148261	5	11	theme	permanent	1062:1070	arg1	strain					1072:1077	permanent strain	1062:1077	permanent strain	1062:1077	Young's modulus, maximum stress/strain, and permanent strain following cyclic loading were determined in ovine vagina.
25148261	10	12	theme	absolute	1785:1792	arg1	content					1794:1800	the absolute content	1781:1800	the absolute content of proteins	1781:1812	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	10	12	theme	absolute	1785:1792	arg1	similar					1819:1825	similar	1819:1825	similar	1819:1825	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	5	13	theme	maximum	1035:1041	arg1	stress/strain					1043:1055	maximum stress/strain	1035:1055	maximum stress/strain	1035:1055	Young's modulus, maximum stress/strain, and permanent strain following cyclic loading were determined in ovine vagina.
25148261	3	14	theme	ovariectomised	606:619	arg1	sheep					621:625	ovariectomised sheep	606:625	ovariectomised sheep (n = 6)	606:633	STUDY DESIGN Vaginal tissue was collected from ovariectomised sheep (n = 6) and from postmenopausal women (n = 7) from the proximal, middle and distal thirds.
25148261	3	14	theme	ovariectomised	606:619	arg1	n = 6					628:632	n = 6	628:632	n = 6	628:632	STUDY DESIGN Vaginal tissue was collected from ovariectomised sheep (n = 6) and from postmenopausal women (n = 7) from the proximal, middle and distal thirds.
25148261	4	15	theme	dimethylmethylene	918:934	arg1	assay					941:945	dimethylmethylene blue assay	918:945	dimethylmethylene blue assay	918:945	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	3	16	theme	STUDY	559:563	arg1	DESIGN					565:570	STUDY DESIGN	559:570	STUDY DESIGN Vaginal tissue	559:585	STUDY DESIGN Vaginal tissue was collected from ovariectomised sheep (n = 6) and from postmenopausal women (n = 7) from the proximal, middle and distal thirds.
25148261	11	17	theme	mechanical	1888:1897	arg1	properties					1899:1908	mechanical properties	1888:1908	mechanical properties	1888:1908	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall will assist future studies using sheep as a model for vaginal surgery.
25148261	5	18	theme	ovine	1123:1127	arg1	vagina					1129:1134	ovine vagina	1123:1134	ovine vagina	1123:1134	Young's modulus, maximum stress/strain, and permanent strain following cyclic loading were determined in ovine vagina.
25148261	1	19	theme	reports	154:160	arg1	numbers					143:149	increasing numbers	132:149	increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results	132:277	OBJECTIVE There are increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results.
25148261	0	20	theme	ovine	88:92	arg1	vagina					104:109	postmenopausal ovine and human vagina	73:109	postmenopausal ovine and human vagina	73:109	Regional variation in tissue composition and biomechanical properties of postmenopausal ovine and human vagina.
25148261	1	21	theme	human	173:177	arg1	composition					194:204	human vaginal tissue composition	173:204	human vaginal tissue composition	173:204	OBJECTIVE There are increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results.
25148261	4	22	theme	tissue	960:965	arg1	ETAP					988:991	ETAP	988:991	ETAP	988:991	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	4	22	theme	tissue	960:965	arg1	proteins					978:985	elastic tissue associated proteins	952:985	elastic tissue associated proteins (ETAP)	952:992	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	4	23	theme	Trichrome	763:771	arg1	staining					773:780	Masson's Trichrome staining	754:780	Masson's Trichrome staining	754:780	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	0	24	theme	human	98:102	arg1	vagina					104:109	postmenopausal ovine and human vagina	73:109	postmenopausal ovine and human vagina	73:109	Regional variation in tissue composition and biomechanical properties of postmenopausal ovine and human vagina.
25148261	9	25	theme	maximum	1526:1532	arg1	stress					1534:1539	maximum stress	1526:1539	maximum stress	1526:1539	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	9	25	theme	maximum	1526:1532	arg1	p<0.05					1542:1547	p<0.05	1542:1547	p<0.05	1542:1547	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	2	26	theme	pelvic	527:532	arg1	research					549:556	pelvic organ prolapse research	527:556	pelvic organ prolapse research	527:556	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	2	27	theme	tissue	399:404	arg1	composition					406:416	histological and biochemical tissue composition	370:416	histological and biochemical tissue composition	370:416	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	11	28	from	Knowledge	1828:1836	arg1	composition					1872:1882	composition	1872:1882	composition	1872:1882	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall will assist future studies using sheep as a model for vaginal surgery.
25148261	11	28	from	Knowledge	1828:1836	arg1	properties					1899:1908	mechanical properties	1888:1908	mechanical properties	1888:1908	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall will assist future studies using sheep as a model for vaginal surgery.
25148261	0	29	theme	Regional	0:7	arg1	variation					9:17	Regional variation	0:17	Regional variation in tissue composition and biomechanical properties of postmenopausal ovine and human vagina	0:109	Regional variation in tissue composition and biomechanical properties of postmenopausal ovine and human vagina.
25148261	11	30	dep	composition	1872:1882	arg1	the					1868:1870	the	1868:1870	the	1868:1870	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall will assist future studies using sheep as a model for vaginal surgery.
25148261	2	31	theme	biomechanical	440:452	arg1	properties					454:463	passive biomechanical properties	432:463	passive biomechanical properties of ovine vagina	432:479	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	8	32	theme	human	1403:1407	arg1	vagina					1409:1414	the human vagina	1399:1414	the human vagina	1399:1414	No significant differences were found along the length of the human vagina for collagen, GAG or ETAP content.
25148261	5	33	theme	Young	1018:1022	arg1	modulus					1026:1032	Young's modulus	1018:1032	Young's modulus	1018:1032	Young's modulus, maximum stress/strain, and permanent strain following cyclic loading were determined in ovine vagina.
25148261	11	34	theme	vaginal	1917:1923	arg1	wall					1925:1928	the vaginal wall	1913:1928	the vaginal wall	1913:1928	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall will assist future studies using sheep as a model for vaginal surgery.
25148261	4	35	theme	acid	1003:1006	arg1	analysis					1008:1015	amino acid analysis	997:1015	amino acid analysis	997:1015	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	7	36	dep	showed	1240:1245	arg1	nearest					1321:1327	nearest	1321:1327	showed significantly higher total collagen and glycosaminoglycan values (p<0.05) nearest the cervix	1240:1338	Ovine vaginal tissue showed significantly higher total collagen and glycosaminoglycan values (p<0.05) nearest the cervix.
25148261	4	37	theme	hydroxyproline	816:829	arg1	assays					831:836	hydroxyproline assays	816:836	hydroxyproline assays	816:836	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	10	38	theme	tissue	1631:1636	arg1	composition					1638:1648	CONCLUSION Sheep tissue composition	1614:1648	CONCLUSION Sheep tissue composition	1614:1648	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	2	39	theme	vagina	474:479	arg1	properties					454:463	passive biomechanical properties	432:463	passive biomechanical properties of ovine vagina	432:479	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	2	40	theme	organ	534:538	arg1	research					549:556	pelvic organ prolapse research	527:556	pelvic organ prolapse research	527:556	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	2	41	theme	vaginal	343:349	arg1	tissue					351:356	ovine and human posterior vaginal tissue	317:356	ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition	317:416	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	0	42	theme	biomechanical	45:57	arg1	properties					59:68	biomechanical properties	45:68	biomechanical properties	45:68	Regional variation in tissue composition and biomechanical properties of postmenopausal ovine and human vagina.
25148261	3	43	theme	proximal	682:689	arg1	thirds					710:715	the proximal, middle and distal thirds	678:715	the proximal, middle and distal thirds	678:715	STUDY DESIGN Vaginal tissue was collected from ovariectomised sheep (n = 6) and from postmenopausal women (n = 7) from the proximal, middle and distal thirds.
25148261	9	44	dep	stiffest	1479:1486	arg1	modulus					1497:1503	Young's modulus	1489:1503	Young's modulus	1489:1503	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	9	44	dep	stiffest	1479:1486	arg1	p<0.05					1506:1511	p<0.05	1506:1511	p<0.05	1506:1511	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	2	45	theme	human	327:331	arg1	tissue					351:356	ovine and human posterior vaginal tissue	317:356	ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition	317:416	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	1	46	theme	pelvic	232:237	arg1	prolapse					245:252	pelvic organ prolapse	232:252	pelvic organ prolapse with conflicting results	232:277	OBJECTIVE There are increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results.
25148261	1	47	with	prolapse	245:252	arg1	results					271:277	conflicting results	259:277	conflicting results	259:277	OBJECTIVE There are increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results.
25148261	10	48	theme	Sheep	1625:1629	arg1	composition					1638:1648	CONCLUSION Sheep tissue composition	1614:1648	CONCLUSION Sheep tissue composition	1614:1648	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	6	49	dep	RESULTS	1137:1143	arg1	tissue					1174:1179	human vaginal tissue	1160:1179	human vaginal tissue	1160:1179	RESULTS Both sheep and human vaginal tissue showed comparable tissue composition.
25148261	6	49	dep	RESULTS	1137:1143	arg1	sheep					1150:1154	sheep	1150:1154	sheep	1150:1154	RESULTS Both sheep and human vaginal tissue showed comparable tissue composition.
25148261	6	49	dep	RESULTS	1137:1143	arg1	RESULTS					1137:1143	RESULTS Both sheep and human vaginal tissue	1137:1179	RESULTS Both sheep and human vaginal tissue	1137:1179	RESULTS Both sheep and human vaginal tissue showed comparable tissue composition.
25148261	10	50	theme	postmenopausal	1714:1727	arg1	wall					1737:1740	the postmenopausal vaginal wall	1710:1740	the postmenopausal vaginal wall	1710:1740	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	9	51	theme	permanent	1595:1603	arg1	elastic					1586:1592	elastic	1586:1592	elastic	1586:1592	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	9	51	theme	permanent	1595:1603	arg1	strain					1605:1610	permanent strain	1595:1610	permanent strain	1595:1610	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	3	52	theme	distal	703:708	arg1	thirds					710:715	the proximal, middle and distal thirds	678:715	the proximal, middle and distal thirds	678:715	STUDY DESIGN Vaginal tissue was collected from ovariectomised sheep (n = 6) and from postmenopausal women (n = 7) from the proximal, middle and distal thirds.
25148261	11	53	theme	baseline	1846:1853	arg1	variation					1855:1863	this baseline variation	1841:1863	this baseline variation	1841:1863	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall will assist future studies using sheep as a model for vaginal surgery.
25148261	1	54	theme	conflicting	259:269	arg1	results					271:277	conflicting results	259:277	conflicting results	259:277	OBJECTIVE There are increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results.
25148261	4	55	theme	delayed	868:874	arg1	PAGE					890:893	delayed reduction SDS PAGE	868:893	delayed reduction SDS PAGE	868:893	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	7	56	theme	total	1268:1272	arg1	collagen					1274:1281	total collagen	1268:1281	total collagen	1268:1281	Ovine vaginal tissue showed significantly higher total collagen and glycosaminoglycan values (p<0.05) nearest the cervix.
25148261	3	57	theme	middle	692:697	arg1	thirds					710:715	the proximal, middle and distal thirds	678:715	the proximal, middle and distal thirds	678:715	STUDY DESIGN Vaginal tissue was collected from ovariectomised sheep (n = 6) and from postmenopausal women (n = 7) from the proximal, middle and distal thirds.
25148261	2	58	theme	study	296:300	arg1	aim					284:286	The aim	280:286	The aim of this study	280:300	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	4	59	theme	SDS	886:888	arg1	PAGE					890:893	delayed reduction SDS PAGE	868:893	delayed reduction SDS PAGE	868:893	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	10	60	theme	regional	1683:1690	arg1	differences					1692:1702	regional differences	1683:1702	regional differences along the postmenopausal vaginal wall not apparent	1683:1753	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	1	61	theme	vaginal	179:185	arg1	composition					194:204	human vaginal tissue composition	173:204	human vaginal tissue composition	173:204	OBJECTIVE There are increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results.
25148261	4	62	theme	collagen	839:846	arg1	ratios					858:863	collagen III/I+III ratios	839:863	collagen III/I+III ratios by delayed reduction SDS PAGE	839:893	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	6	63	theme	vaginal	1166:1172	arg1	tissue					1174:1179	human vaginal tissue	1160:1179	human vaginal tissue	1160:1179	RESULTS Both sheep and human vaginal tissue showed comparable tissue composition.
25148261	6	63	theme	vaginal	1166:1172	arg1	RESULTS					1137:1143	RESULTS Both sheep and human vaginal tissue	1137:1179	RESULTS Both sheep and human vaginal tissue	1137:1179	RESULTS Both sheep and human vaginal tissue showed comparable tissue composition.
25148261	10	64	theme	apparent	1746:1753	arg1	differences					1692:1702	regional differences	1683:1702	regional differences along the postmenopausal vaginal wall not apparent	1683:1753	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	2	65	theme	ovine	317:321	arg1	tissue					351:356	ovine and human posterior vaginal tissue	317:356	ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition	317:416	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	9	66	theme	proximal	1455:1462	arg1	region					1464:1469	The proximal region	1451:1469	The proximal region	1451:1469	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	9	66	theme	proximal	1455:1462	arg1	stiffest					1479:1486	the stiffest	1475:1486	the stiffest (Young's modulus, p<0.05)	1475:1512	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	3	67	theme	Vaginal	572:578	arg1	tissue					580:585	STUDY DESIGN Vaginal tissue	559:585	STUDY DESIGN Vaginal tissue	559:585	STUDY DESIGN Vaginal tissue was collected from ovariectomised sheep (n = 6) and from postmenopausal women (n = 7) from the proximal, middle and distal thirds.
25148261	4	68	theme	blue	936:939	arg1	assay					941:945	dimethylmethylene blue assay	918:945	dimethylmethylene blue assay	918:945	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	11	69	theme	future	1942:1947	arg1	studies					1949:1955	future studies	1942:1955	future studies using sheep as a model for vaginal surgery	1942:1998	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall will assist future studies using sheep as a model for vaginal surgery.
25148261	2	70	theme	prolapse	540:547	arg1	research					549:556	pelvic organ prolapse research	527:556	pelvic organ prolapse research	527:556	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	5	71	theme	cyclic	1089:1094	arg1	loading					1096:1102	cyclic loading	1089:1102	cyclic loading	1089:1102	Young's modulus, maximum stress/strain, and permanent strain following cyclic loading were determined in ovine vagina.
25148261	9	72	theme	Young	1489:1493	arg1	modulus					1497:1503	Young's modulus	1489:1503	Young's modulus	1489:1503	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	9	72	theme	Young	1489:1493	arg1	p<0.05					1506:1511	p<0.05	1506:1511	p<0.05	1506:1511	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	1	73	theme	increasing	132:141	arg1	numbers					143:149	increasing numbers	132:149	increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results	132:277	OBJECTIVE There are increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results.
25148261	10	74	theme	human	1758:1762	arg1	vagina					1764:1769	human vagina	1758:1769	human vagina	1758:1769	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	4	75	theme	Tissue	718:723	arg1	histology					725:733	Tissue histology	718:733	Tissue histology	718:733	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	0	76	theme	postmenopausal	73:86	arg1	vagina					104:109	postmenopausal ovine and human vagina	73:109	postmenopausal ovine and human vagina	73:109	Regional variation in tissue composition and biomechanical properties of postmenopausal ovine and human vagina.
25148261	10	77	theme	proteins	1805:1812	arg1	content					1794:1800	the absolute content	1781:1800	the absolute content of proteins	1781:1812	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	10	77	theme	proteins	1805:1812	arg1	similar					1819:1825	similar	1819:1825	similar	1819:1825	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	2	78	theme	animal	510:515	arg1	model					517:521	this animal model	505:521	this animal model for pelvic organ prolapse research	505:556	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	6	79	theme	tissue	1199:1204	arg1	composition					1206:1216	comparable tissue composition	1188:1216	comparable tissue composition	1188:1216	RESULTS Both sheep and human vaginal tissue showed comparable tissue composition.
25148261	1	80	dep	OBJECTIVE	112:120	arg1	are					128:130	are	128:130	are increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results	128:277	OBJECTIVE There are increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results.
25148261	7	81	theme	Ovine	1219:1223	arg1	tissue					1233:1238	Ovine vaginal tissue	1219:1238	Ovine vaginal tissue	1219:1238	Ovine vaginal tissue showed significantly higher total collagen and glycosaminoglycan values (p<0.05) nearest the cervix.
25148261	7	82	theme	vaginal	1225:1231	arg1	tissue					1233:1238	Ovine vaginal tissue	1219:1238	Ovine vaginal tissue	1219:1238	Ovine vaginal tissue showed significantly higher total collagen and glycosaminoglycan values (p<0.05) nearest the cervix.
25148261	10	83	theme	CONCLUSION	1614:1623	arg1	composition					1638:1648	CONCLUSION Sheep tissue composition	1614:1648	CONCLUSION Sheep tissue composition	1614:1648	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	0	84	from	variation	9:17	arg1	composition					29:39	tissue composition	22:39	tissue composition	22:39	Regional variation in tissue composition and biomechanical properties of postmenopausal ovine and human vagina.
25148261	0	84	from	variation	9:17	arg1	properties					59:68	biomechanical properties	45:68	biomechanical properties	45:68	Regional variation in tissue composition and biomechanical properties of postmenopausal ovine and human vagina.
25148261	8	85	theme	significant	1344:1354	arg1	differences					1356:1366	No significant differences	1341:1366	No significant differences	1341:1366	No significant differences were found along the length of the human vagina for collagen, GAG or ETAP content.
25148261	0	86	theme	vagina	104:109	arg1	composition					29:39	tissue composition	22:39	tissue composition	22:39	Regional variation in tissue composition and biomechanical properties of postmenopausal ovine and human vagina.
25148261	0	86	theme	vagina	104:109	arg1	properties					59:68	biomechanical properties	45:68	biomechanical properties	45:68	Regional variation in tissue composition and biomechanical properties of postmenopausal ovine and human vagina.
25148261	4	87	theme	elastic	952:958	arg1	ETAP					988:991	ETAP	988:991	ETAP	988:991	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	4	87	theme	elastic	952:958	arg1	proteins					978:985	elastic tissue associated proteins	952:985	elastic tissue associated proteins (ETAP)	952:992	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	4	88	theme	associated	967:976	arg1	ETAP					988:991	ETAP	988:991	ETAP	988:991	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	4	88	theme	associated	967:976	arg1	proteins					978:985	elastic tissue associated proteins	952:985	elastic tissue associated proteins (ETAP)	952:992	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	2	89	theme	biochemical	387:397	arg1	composition					406:416	histological and biochemical tissue composition	370:416	histological and biochemical tissue composition	370:416	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	9	90	dep	strongest	1515:1523	arg1	stress					1534:1539	maximum stress	1526:1539	maximum stress	1526:1539	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	9	90	dep	strongest	1515:1523	arg1	p<0.05					1542:1547	p<0.05	1542:1547	p<0.05	1542:1547	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	2	91	theme	histological	370:381	arg1	composition					406:416	histological and biochemical tissue composition	370:416	histological and biochemical tissue composition	370:416	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	0	92	theme	tissue	22:27	arg1	composition					29:39	tissue composition	22:39	tissue composition	22:39	Regional variation in tissue composition and biomechanical properties of postmenopausal ovine and human vagina.
25148261	2	93	theme	passive	432:438	arg1	properties					454:463	passive biomechanical properties	432:463	passive biomechanical properties of ovine vagina	432:479	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	11	94	theme	wall	1925:1928	arg1	composition					1872:1882	composition	1872:1882	composition	1872:1882	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall will assist future studies using sheep as a model for vaginal surgery.
25148261	11	94	theme	wall	1925:1928	arg1	properties					1899:1908	mechanical properties	1888:1908	mechanical properties	1888:1908	Knowledge of this baseline variation in the composition and mechanical properties of the vaginal wall will assist future studies using sheep as a model for vaginal surgery.
25148261	9	95	theme	distal	1562:1567	arg1	region					1569:1574	distal region	1562:1574	distal region	1562:1574	The proximal region was the stiffest (Young's modulus, p<0.05), strongest (maximum stress, p<0.05) compared to distal region, and most elastic (permanent strain).
25148261	4	96	theme	amino	997:1001	arg1	analysis					1008:1015	amino acid analysis	997:1015	amino acid analysis	997:1015	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	3	97	theme	postmenopausal	644:657	arg1	n = 7					666:670	n = 7	666:670	n = 7	666:670	STUDY DESIGN Vaginal tissue was collected from ovariectomised sheep (n = 6) and from postmenopausal women (n = 7) from the proximal, middle and distal thirds.
25148261	3	97	theme	postmenopausal	644:657	arg1	women					659:663	postmenopausal women	644:663	postmenopausal women (n = 7)	644:671	STUDY DESIGN Vaginal tissue was collected from ovariectomised sheep (n = 6) and from postmenopausal women (n = 7) from the proximal, middle and distal thirds.
25148261	8	98	theme	vagina	1409:1414	arg1	length					1389:1394	the length	1385:1394	the length of the human vagina for collagen, GAG or ETAP content	1385:1448	No significant differences were found along the length of the human vagina for collagen, GAG or ETAP content.
25148261	2	99	theme	composition	406:416	arg1	terms					361:365	terms	361:365	terms of histological and biochemical tissue composition	361:416	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	2	100	theme	ovine	468:472	arg1	vagina					474:479	ovine vagina	468:479	ovine vagina	468:479	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	2	101	theme	posterior	333:341	arg1	tissue					351:356	ovine and human posterior vaginal tissue	317:356	ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition	317:416	The aim of this study was to compare ovine and human posterior vaginal tissue in terms of histological and biochemical tissue composition and to assess passive biomechanical properties of ovine vagina to further characterise this animal model for pelvic organ prolapse research.
25148261	7	102	theme	glycosaminoglycan	1287:1303	arg1	values					1305:1310	significantly higher total collagen and glycosaminoglycan values	1247:1310	significantly higher total collagen and glycosaminoglycan values (p<0.05)	1247:1319	Ovine vaginal tissue showed significantly higher total collagen and glycosaminoglycan values (p<0.05) nearest the cervix.
25148261	7	102	theme	glycosaminoglycan	1287:1303	arg1	p<0.05					1313:1318	p<0.05	1313:1318	p<0.05	1313:1318	Ovine vaginal tissue showed significantly higher total collagen and glycosaminoglycan values (p<0.05) nearest the cervix.
25148261	4	103	theme	total	783:787	arg1	collagen					789:796	total collagen	783:796	total collagen	783:796	Tissue histology was analyzed using Masson's Trichrome staining; total collagen was quantified by hydroxyproline assays, collagen III/I+III ratios by delayed reduction SDS PAGE, glycosaminoglycans by dimethylmethylene blue assay, and elastic tissue associated proteins (ETAP) by amino acid analysis.
25148261	10	104	theme	mechanical	1654:1663	arg1	properties					1665:1674	mechanical properties	1654:1674	mechanical properties	1654:1674	CONCLUSION Sheep tissue composition and mechanical properties showed regional differences along the postmenopausal vaginal wall not apparent in human vagina, although the absolute content of proteins were similar.
25148261	1	105	theme	organ	239:243	arg1	prolapse					245:252	pelvic organ prolapse	232:252	pelvic organ prolapse with conflicting results	232:277	OBJECTIVE There are increasing numbers of reports describing human vaginal tissue composition in women with and without pelvic organ prolapse with conflicting results.
25148261	7	106	theme	collagen	1274:1281	arg1	values					1305:1310	significantly higher total collagen and glycosaminoglycan values	1247:1310	significantly higher total collagen and glycosaminoglycan values (p<0.05)	1247:1319	Ovine vaginal tissue showed significantly higher total collagen and glycosaminoglycan values (p<0.05) nearest the cervix.
25148261	7	106	theme	collagen	1274:1281	arg1	p<0.05					1313:1318	p<0.05	1313:1318	p<0.05	1313:1318	Ovine vaginal tissue showed significantly higher total collagen and glycosaminoglycan values (p<0.05) nearest the cervix.
25148261	8	107	theme	ETAP	1437:1440	arg1	content					1442:1448	ETAP content	1437:1448	ETAP content	1437:1448	No significant differences were found along the length of the human vagina for collagen, GAG or ETAP content.
27228950	2	0	from	pasta	394:398	arg1	rates					359:363	the rates	355:363	the rates of their liberation from the pasta under simulated gastric and small intestinal conditions	355:454	We showed that the rates of their liberation from the pasta under simulated gastric and small intestinal conditions largely depended upon the rate of digestion of the starchy matrix.
27228950	2	0	from	pasta	394:398	arg1	liberation					374:383	their liberation	368:383	their liberation from the pasta under simulated gastric and small intestinal conditions	368:454	We showed that the rates of their liberation from the pasta under simulated gastric and small intestinal conditions largely depended upon the rate of digestion of the starchy matrix.
27228950	6	1	with	pellets	1106:1112	arg1	values					1141:1146	their peak values	1130:1146	their peak values occurring synchronously during the first hour	1130:1192	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	0	2	theme	absorption	58:67	arg1	matrices					100:107	other food matrices	89:107	other food matrices in human subjects	89:125	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	0	2	theme	absorption	58:67	arg1	rates					33:37	real time rates	23:37	real time rates of dissolution and absorption of carbohydrate	23:83	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	6	3	from	patterns	988:995	arg1	insulin					1037:1043	insulin	1037:1043	insulin	1037:1043	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	6	3	from	patterns	988:995	arg1	glucose					1026:1032	serum glucose	1020:1032	serum glucose	1020:1032	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	8	4	theme	digestion	1592:1600	arg1	prolongation					1576:1587	the prolongation	1572:1587	the prolongation of digestion of larger particles	1572:1620	Further, the transit times calculated on a basis of the ratios of the two marker sugars could identify that the prolongation of digestion of larger particles was not accompanied by retention of digesta in particular segments of the gut.
27228950	2	5	theme	starchy	507:513	arg1	matrix					515:520	the starchy matrix	503:520	the starchy matrix	503:520	We showed that the rates of their liberation from the pasta under simulated gastric and small intestinal conditions largely depended upon the rate of digestion of the starchy matrix.
27228950	7	6	theme	reproducible	1278:1289	arg1	means					1305:1309	a reproducible and practical means	1276:1309	a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix	1276:1373	Hence feeding test foods impregnated with lactulose and mannitol probes provided a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix and showed that this was more protracted than suggested by post prandial glucose levels.
27228950	4	7	theme	solution	709:716	arg1	consumption					654:664	consumption	654:664	consumption of either the powdered pasta or the simple solution of probe sugars	654:732	Hence, after consumption of either the powdered pasta or the simple solution of probe sugars, the mass of mannitol excreted between 1 and 2½ hours was greater than that excreted between 2½ and 4 hours.
27228950	7	8	theme	carbohydrate	1355:1366	arg1	matrix					1368:1373	the carbohydrate matrix	1351:1373	the carbohydrate matrix	1351:1373	Hence feeding test foods impregnated with lactulose and mannitol probes provided a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix and showed that this was more protracted than suggested by post prandial glucose levels.
27228950	6	9	with	powder	1118:1123	arg1	values					1141:1146	their peak values	1130:1146	their peak values occurring synchronously during the first hour	1130:1192	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	0	10	theme	carbohydrate	72:83	arg1	absorption					58:67	absorption	58:67	absorption	58:67	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	0	10	theme	carbohydrate	72:83	arg1	dissolution					42:52	dissolution	42:52	dissolution	42:52	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	6	11	theme	first	1183:1187	arg1	hour					1189:1192	the first hour	1179:1192	the first hour	1179:1192	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	6	12	from	variation	1000:1008	arg1	insulin					1037:1043	insulin	1037:1043	insulin	1037:1043	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	6	12	from	variation	1000:1008	arg1	glucose					1026:1032	serum glucose	1020:1032	serum glucose	1020:1032	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	4	13	theme	simple	702:707	arg1	solution					709:716	the simple solution	698:716	the simple solution of probe sugars	698:732	Hence, after consumption of either the powdered pasta or the simple solution of probe sugars, the mass of mannitol excreted between 1 and 2½ hours was greater than that excreted between 2½ and 4 hours.
27228950	8	14	theme	larger	1605:1610	arg1	particles					1612:1620	larger particles	1605:1620	larger particles	1605:1620	Further, the transit times calculated on a basis of the ratios of the two marker sugars could identify that the prolongation of digestion of larger particles was not accompanied by retention of digesta in particular segments of the gut.
27228950	0	15	theme	food	95:98	arg1	matrices					100:107	other food matrices	89:107	other food matrices in human subjects	89:125	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	0	16	from	rates	33:37	arg1	subjects					118:125	human subjects	112:125	human subjects	112:125	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	6	17	theme	variation	1000:1008	arg1	patterns					988:995	the concurrent patterns	973:995	the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes	973:1066	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	4	18	theme	probe	721:725	arg1	sugars					727:732	probe sugars	721:732	probe sugars	721:732	Hence, after consumption of either the powdered pasta or the simple solution of probe sugars, the mass of mannitol excreted between 1 and 2½ hours was greater than that excreted between 2½ and 4 hours.
27228950	2	19	theme	liberation	374:383	arg1	rates					359:363	the rates	355:363	the rates of their liberation from the pasta under simulated gastric and small intestinal conditions	355:454	We showed that the rates of their liberation from the pasta under simulated gastric and small intestinal conditions largely depended upon the rate of digestion of the starchy matrix.
27228950	0	20	theme	other	89:93	arg1	matrices					100:107	other food matrices	89:107	other food matrices in human subjects	89:125	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	1	21	dep	absorbed	289:296	arg1	excreted					321:328	excreted	321:328	are absorbed passively and promptly excreted in urine	285:337	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	4	22	theme	pasta	689:693	arg1	consumption					654:664	consumption	654:664	consumption of either the powdered pasta or the simple solution of probe sugars	654:732	Hence, after consumption of either the powdered pasta or the simple solution of probe sugars, the mass of mannitol excreted between 1 and 2½ hours was greater than that excreted between 2½ and 4 hours.
27228950	8	23	theme	particles	1612:1620	arg1	digestion					1592:1600	digestion	1592:1600	digestion of larger particles	1592:1620	Further, the transit times calculated on a basis of the ratios of the two marker sugars could identify that the prolongation of digestion of larger particles was not accompanied by retention of digesta in particular segments of the gut.
27228950	1	24	theme	physical	159:166	arg1	dimensions					168:177	physical dimensions	159:177	physical dimensions	159:177	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	3	25	theme	particle	626:633	arg1	size					635:638	the larger particle size	615:638	the larger particle size	615:638	We showed, in 20 female subjects, that excretion of mannitol was slower from the pasta with the larger particle size.
27228950	7	26	theme	test	1209:1212	arg1	foods					1214:1218	Hence feeding test foods	1195:1218	Hence feeding test foods impregnated with lactulose and mannitol probes	1195:1265	Hence feeding test foods impregnated with lactulose and mannitol probes provided a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix and showed that this was more protracted than suggested by post prandial glucose levels.
27228950	6	27	theme	concurrent	977:986	arg1	patterns					988:995	the concurrent patterns	973:995	the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes	973:1066	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	2	28	theme	gastric	416:422	arg1	conditions					445:454	simulated gastric and small intestinal conditions	406:454	simulated gastric and small intestinal conditions	406:454	We showed that the rates of their liberation from the pasta under simulated gastric and small intestinal conditions largely depended upon the rate of digestion of the starchy matrix.
27228950	4	29	theme	powdered	680:687	arg1	pasta					689:693	the powdered pasta	676:693	the powdered pasta	676:693	Hence, after consumption of either the powdered pasta or the simple solution of probe sugars, the mass of mannitol excreted between 1 and 2½ hours was greater than that excreted between 2½ and 4 hours.
27228950	3	30	theme	female	540:545	arg1	subjects					547:554	20 female subjects	537:554	20 female subjects	537:554	We showed, in 20 female subjects, that excretion of mannitol was slower from the pasta with the larger particle size.
27228950	8	31	theme	transit	1477:1483	arg1	times					1485:1489	the transit times	1473:1489	the transit times calculated on a basis of the ratios of the two marker sugars	1473:1550	Further, the transit times calculated on a basis of the ratios of the two marker sugars could identify that the prolongation of digestion of larger particles was not accompanied by retention of digesta in particular segments of the gut.
27228950	7	32	theme	digestion	1338:1346	arg1	timing					1328:1333	the timing	1324:1333	the timing of digestion of the carbohydrate matrix	1324:1373	Hence feeding test foods impregnated with lactulose and mannitol probes provided a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix and showed that this was more protracted than suggested by post prandial glucose levels.
27228950	1	33	theme	identical	183:191	arg1	composition					202:212	identical chemical composition	183:212	identical chemical composition	183:212	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	0	34	theme	time	28:31	arg1	rates					33:37	real time rates	23:37	real time rates of dissolution and absorption of carbohydrate	23:83	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	5	35	theme	pellets	924:930	arg1	consumption					899:909	consumption	899:909	consumption of the pasta pellets	899:930	However these masses did not differ significantly after consumption of the pasta pellets.
27228950	7	36	theme	matrix	1368:1373	arg1	digestion					1338:1346	digestion	1338:1346	digestion of the carbohydrate matrix	1338:1373	Hence feeding test foods impregnated with lactulose and mannitol probes provided a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix and showed that this was more protracted than suggested by post prandial glucose levels.
27228950	7	37	theme	feeding	1201:1207	arg1	foods					1214:1218	Hence feeding test foods	1195:1218	Hence feeding test foods impregnated with lactulose and mannitol probes	1195:1265	Hence feeding test foods impregnated with lactulose and mannitol probes provided a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix and showed that this was more protracted than suggested by post prandial glucose levels.
27228950	4	38	theme	mannitol	747:754	arg1	greater					792:798	greater	792:798	greater	792:798	Hence, after consumption of either the powdered pasta or the simple solution of probe sugars, the mass of mannitol excreted between 1 and 2½ hours was greater than that excreted between 2½ and 4 hours.
27228950	4	38	theme	mannitol	747:754	arg1	mass					739:742	the mass	735:742	the mass of mannitol excreted between 1 and 2½ hours	735:786	Hence, after consumption of either the powdered pasta or the simple solution of probe sugars, the mass of mannitol excreted between 1 and 2½ hours was greater than that excreted between 2½ and 4 hours.
27228950	6	39	theme	pasta	1100:1104	arg1	pellets					1106:1112	pasta pellets	1100:1112	pasta pellets	1100:1112	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	1	40	theme	chemical	193:200	arg1	composition					202:212	identical chemical composition	183:212	identical chemical composition	183:212	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	0	41	theme	real	23:26	arg1	rates					33:37	real time rates	23:37	real time rates of dissolution and absorption of carbohydrate	23:83	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	0	42	theme	human	112:116	arg1	subjects					118:125	human subjects	112:125	human subjects	112:125	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	7	43	theme	prandial	1439:1446	arg1	levels					1456:1461	post prandial glucose levels	1434:1461	post prandial glucose levels	1434:1461	Hence feeding test foods impregnated with lactulose and mannitol probes provided a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix and showed that this was more protracted than suggested by post prandial glucose levels.
27228950	6	44	theme	peak	1136:1139	arg1	values					1141:1146	their peak values	1130:1146	their peak values occurring synchronously during the first hour	1130:1192	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	6	45	from	insulin	1037:1043	arg1	patterns					988:995	the concurrent patterns	973:995	the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes	973:1066	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	2	46	theme	digestion	490:498	arg1	rate					482:485	the rate	478:485	the rate of digestion of the starchy matrix	478:520	We showed that the rates of their liberation from the pasta under simulated gastric and small intestinal conditions largely depended upon the rate of digestion of the starchy matrix.
27228950	7	47	theme	glucose	1448:1454	arg1	levels					1456:1461	post prandial glucose levels	1434:1461	post prandial glucose levels	1434:1461	Hence feeding test foods impregnated with lactulose and mannitol probes provided a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix and showed that this was more protracted than suggested by post prandial glucose levels.
27228950	1	48	contain	contained	219:227	arg2	lactulose					256:264	lactulose	256:264	lactulose	256:264	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	1	48	contain	contained	219:227	arg1	dimensions					168:177	physical dimensions	159:177	physical dimensions	159:177	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	1	48	contain	contained	219:227	arg2	probes					248:253	two monosaccharide probes	229:253	two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine	229:337	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	1	48	contain	contained	219:227	arg2	mannitol					270:277	mannitol	270:277	mannitol	270:277	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	1	48	contain	contained	219:227	arg1	composition					202:212	identical chemical composition	183:212	identical chemical composition	183:212	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	2	49	from	rates	359:363	arg1	pasta					394:398	the pasta	390:398	the pasta	390:398	We showed that the rates of their liberation from the pasta under simulated gastric and small intestinal conditions largely depended upon the rate of digestion of the starchy matrix.
27228950	2	50	theme	matrix	515:520	arg1	digestion					490:498	digestion	490:498	digestion of the starchy matrix	490:520	We showed that the rates of their liberation from the pasta under simulated gastric and small intestinal conditions largely depended upon the rate of digestion of the starchy matrix.
27228950	2	51	theme	intestinal	434:443	arg1	conditions					445:454	simulated gastric and small intestinal conditions	406:454	simulated gastric and small intestinal conditions	406:454	We showed that the rates of their liberation from the pasta under simulated gastric and small intestinal conditions largely depended upon the rate of digestion of the starchy matrix.
27228950	7	52	theme	practical	1295:1303	arg1	means					1305:1309	a reproducible and practical means	1276:1309	a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix	1276:1373	Hence feeding test foods impregnated with lactulose and mannitol probes provided a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix and showed that this was more protracted than suggested by post prandial glucose levels.
27228950	8	53	theme	particular	1669:1678	arg1	segments					1680:1687	particular segments	1669:1687	particular segments of the gut	1669:1698	Further, the transit times calculated on a basis of the ratios of the two marker sugars could identify that the prolongation of digestion of larger particles was not accompanied by retention of digesta in particular segments of the gut.
27228950	3	54	theme	mannitol	575:582	arg1	excretion					562:570	excretion	562:570	excretion of mannitol	562:582	We showed, in 20 female subjects, that excretion of mannitol was slower from the pasta with the larger particle size.
27228950	8	55	theme	digesta	1658:1664	arg1	retention					1645:1653	retention	1645:1653	retention of digesta in particular segments of the gut	1645:1698	Further, the transit times calculated on a basis of the ratios of the two marker sugars could identify that the prolongation of digestion of larger particles was not accompanied by retention of digesta in particular segments of the gut.
27228950	7	56	theme	post	1434:1437	arg1	levels					1456:1461	post prandial glucose levels	1434:1461	post prandial glucose levels	1434:1461	Hence feeding test foods impregnated with lactulose and mannitol probes provided a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix and showed that this was more protracted than suggested by post prandial glucose levels.
27228950	8	57	from	retention	1645:1653	arg1	segments					1680:1687	particular segments	1669:1687	particular segments of the gut	1669:1698	Further, the transit times calculated on a basis of the ratios of the two marker sugars could identify that the prolongation of digestion of larger particles was not accompanied by retention of digesta in particular segments of the gut.
27228950	7	58	dep	lactulose	1237:1245	arg1	probes					1260:1265	probes	1260:1265	probes	1260:1265	Hence feeding test foods impregnated with lactulose and mannitol probes provided a reproducible and practical means of assessing the timing of digestion of the carbohydrate matrix and showed that this was more protracted than suggested by post prandial glucose levels.
27228950	8	59	theme	gut	1696:1698	arg1	segments					1680:1687	particular segments	1669:1687	particular segments of the gut	1669:1698	Further, the transit times calculated on a basis of the ratios of the two marker sugars could identify that the prolongation of digestion of larger particles was not accompanied by retention of digesta in particular segments of the gut.
27228950	1	60	dep	probes	248:253	arg1	mannitol					270:277	mannitol	270:277	mannitol	270:277	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	1	60	dep	probes	248:253	arg1	lactulose					256:264	lactulose	256:264	lactulose	256:264	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	1	60	dep	probes	248:253	arg1	probes					248:253	two monosaccharide probes	229:253	two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine	229:337	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	4	61	theme	sugars	727:732	arg1	solution					709:716	the simple solution	698:716	the simple solution of probe sugars	698:732	Hence, after consumption of either the powdered pasta or the simple solution of probe sugars, the mass of mannitol excreted between 1 and 2½ hours was greater than that excreted between 2½ and 4 hours.
27228950	4	61	theme	sugars	727:732	arg1	pasta					689:693	the powdered pasta	676:693	the powdered pasta	676:693	Hence, after consumption of either the powdered pasta or the simple solution of probe sugars, the mass of mannitol excreted between 1 and 2½ hours was greater than that excreted between 2½ and 4 hours.
27228950	3	62	theme	larger	619:624	arg1	size					635:638	the larger particle size	615:638	the larger particle size	615:638	We showed, in 20 female subjects, that excretion of mannitol was slower from the pasta with the larger particle size.
27228950	5	63	theme	pasta	918:922	arg1	pellets					924:930	the pasta pellets	914:930	the pasta pellets	914:930	However these masses did not differ significantly after consumption of the pasta pellets.
27228950	8	64	theme	sugars	1545:1550	arg1	ratios					1520:1525	the ratios	1516:1525	the ratios of the two marker sugars	1516:1550	Further, the transit times calculated on a basis of the ratios of the two marker sugars could identify that the prolongation of digestion of larger particles was not accompanied by retention of digesta in particular segments of the gut.
27228950	8	65	theme	ratios	1520:1525	arg1	basis					1507:1511	a basis	1505:1511	a basis of the ratios of the two marker sugars	1505:1550	Further, the transit times calculated on a basis of the ratios of the two marker sugars could identify that the prolongation of digestion of larger particles was not accompanied by retention of digesta in particular segments of the gut.
27228950	0	66	theme	dissolution	42:52	arg1	matrices					100:107	other food matrices	89:107	other food matrices in human subjects	89:125	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	0	66	theme	dissolution	42:52	arg1	rates					33:37	real time rates	23:37	real time rates of dissolution and absorption of carbohydrate	23:83	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	0	67	from	matrices	100:107	arg1	subjects					118:125	human subjects	112:125	human subjects	112:125	A method for assessing real time rates of dissolution and absorption of carbohydrate and other food matrices in human subjects.
27228950	6	68	from	glucose	1026:1032	arg1	patterns					988:995	the concurrent patterns	973:995	the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes	973:1066	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	6	69	theme	serum	1020:1024	arg1	glucose					1026:1032	serum glucose	1020:1032	serum glucose	1020:1032	These differences were not reflected in the concurrent patterns of variation in either serum glucose or insulin taken over 120 minutes, their levels being similar for pasta pellets and powder with their peak values occurring synchronously during the first hour.
27228950	8	70	theme	marker	1538:1543	arg1	sugars					1545:1550	the two marker sugars	1530:1550	the two marker sugars	1530:1550	Further, the transit times calculated on a basis of the ratios of the two marker sugars could identify that the prolongation of digestion of larger particles was not accompanied by retention of digesta in particular segments of the gut.
27228950	1	71	theme	monosaccharide	233:246	arg1	mannitol					270:277	mannitol	270:277	mannitol	270:277	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	1	71	theme	monosaccharide	233:246	arg1	lactulose					256:264	lactulose	256:264	lactulose	256:264	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
27228950	1	71	theme	monosaccharide	233:246	arg1	probes					248:253	two monosaccharide probes	229:253	two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine	229:337	We prepared pasta of differing physical dimensions but identical chemical composition that contained two monosaccharide probes (lactulose and mannitol) that are absorbed passively and promptly excreted in urine.
26144888	3	0	theme	non-stressed	952:963	arg1	condition					973:981	a non-stressed control condition	950:981	a non-stressed control condition	950:981	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	5	1	from	tests	1238:1242	arg1	mice					1255:1258	control mice	1247:1258	control mice	1247:1258	The stressor resulted in anxiety-like behavior in both the light/dark preference and open field tests in control mice.
26144888	1	2	theme	central	261:267	arg1	CNS					285:287	CNS	285:287	CNS	285:287	There are extensive bidirectional interactions between the gut microbiota and the central nervous system (CNS), and studies demonstrate that stressor exposure significantly alters gut microbiota community structure.
26144888	1	2	theme	central	261:267	arg1	system					277:282	the central nervous system	257:282	the central nervous system (CNS)	257:288	There are extensive bidirectional interactions between the gut microbiota and the central nervous system (CNS), and studies demonstrate that stressor exposure significantly alters gut microbiota community structure.
26144888	7	3	theme	stressor	1463:1470	arg1	exposure					1472:1479	stressor exposure	1463:1479	stressor exposure	1463:1479	These effects were not evident in mice fed milk oligosaccharides; stressor exposure did not significantly change microbial community structure in mice fed 3'SL or 6'SL.
26144888	2	4	theme	stressor-induced	600:615	arg1	alterations					617:627	stressor-induced alterations	600:627	stressor-induced alterations in gut microbial community composition	600:666	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	4	5	theme	mucosa-associated	1053:1069	arg1	microbiota					1071:1080	the colonic mucosa-associated microbiota	1041:1080	the colonic mucosa-associated microbiota	1041:1080	Stressor exposure significantly changed the structure of the colonic mucosa-associated microbiota in control mice, as indicated by changes in beta diversity.
26144888	3	6	theme	laboratory	765:774	arg1	diet					776:779	laboratory diet	765:779	laboratory diet	765:779	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	8	7	theme	behavior	1650:1657	arg1	tests					1628:1632	tests	1628:1632	tests of anxiety-like behavior and normal numbers of DCX+ immature neurons	1628:1701	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
26144888	7	8	from	mice	1431:1434	arg1	evident					1420:1426	evident	1420:1426	evident	1420:1426	These effects were not evident in mice fed milk oligosaccharides; stressor exposure did not significantly change microbial community structure in mice fed 3'SL or 6'SL.
26144888	2	9	theme	microbial	636:644	arg1	composition					656:666	gut microbial community composition	632:666	gut microbial community composition	632:666	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	9	10	theme	microbiota-brain	1881:1896	arg1	axis					1898:1901	the gut microbiota-brain axis	1873:1901	the gut microbiota-brain axis	1873:1901	These studies indicate that milk oligosaccharides support normal microbial communities and behavioral responses during stressor exposure, potentially through effects on the gut microbiota-brain axis.
26144888	3	11	theme	human	796:800	arg1	Sialyllactose					852:864	6'Sialyllactose	850:864	6'Sialyllactose (6'SL)	850:871	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	11	theme	human	796:800	arg1	oligosaccharides					807:822	the human milk oligosaccharides	792:822	the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL)	792:871	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	11	theme	human	796:800	arg1	Sialyllactose					826:838	3'Sialyllactose	824:838	3'Sialyllactose (3'SL)	824:845	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	5	12	from	behavior	1180:1187	arg1	preference					1212:1221	the light/dark preference	1197:1221	the light/dark preference	1197:1221	The stressor resulted in anxiety-like behavior in both the light/dark preference and open field tests in control mice.
26144888	5	12	from	behavior	1180:1187	arg1	tests					1238:1242	open field tests	1227:1242	open field tests in control mice	1227:1258	The stressor resulted in anxiety-like behavior in both the light/dark preference and open field tests in control mice.
26144888	8	13	theme	normal	1663:1668	arg1	numbers					1670:1676	normal numbers	1663:1676	normal numbers of DCX+ immature neurons	1663:1701	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
26144888	2	14	theme	beneficial	555:564	arg1	microbes					576:583	beneficial commensal microbes	555:583	beneficial commensal microbes	555:583	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	1	15	theme	bidirectional	199:211	arg1	interactions					213:224	extensive bidirectional interactions	189:224	extensive bidirectional interactions between the gut microbiota	189:251	There are extensive bidirectional interactions between the gut microbiota and the central nervous system (CNS), and studies demonstrate that stressor exposure significantly alters gut microbiota community structure.
26144888	5	16	theme	light/dark	1201:1210	arg1	preference					1212:1221	the light/dark preference	1197:1221	the light/dark preference	1197:1221	The stressor resulted in anxiety-like behavior in both the light/dark preference and open field tests in control mice.
26144888	8	17	theme	immature	1686:1693	arg1	neurons					1695:1701	DCX+ immature neurons	1681:1701	DCX+ immature neurons	1681:1701	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
26144888	4	18	from	structure	1028:1036	arg1	mice					1093:1096	control mice	1085:1096	control mice	1085:1096	Stressor exposure significantly changed the structure of the colonic mucosa-associated microbiota in control mice, as indicated by changes in beta diversity.
26144888	2	19	theme	microbes	576:583	arg1	growth					545:550	the growth	541:550	the growth of beneficial commensal microbes	541:583	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	2	20	theme	impact	504:509	arg1	development					517:527	impact brain development	504:527	impact brain development	504:527	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	1	21	theme	stressor	320:327	arg1	exposure					329:336	stressor exposure	320:336	stressor exposure	320:336	There are extensive bidirectional interactions between the gut microbiota and the central nervous system (CNS), and studies demonstrate that stressor exposure significantly alters gut microbiota community structure.
26144888	9	22	theme	normal	1762:1767	arg1	communities					1779:1789	normal microbial communities	1762:1789	normal microbial communities	1762:1789	These studies indicate that milk oligosaccharides support normal microbial communities and behavioral responses during stressor exposure, potentially through effects on the gut microbiota-brain axis.
26144888	0	23	theme	stressor-induced	60:75	arg1	behavior					90:97	stressor-induced anxiety-like behavior	60:97	stressor-induced anxiety-like behavior	60:97	The prebiotics 3'Sialyllactose and 6'Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis.
26144888	0	24	dep	alterations	122:132	arg1	Evidence					135:142	Evidence	135:142	stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis	60:176	The prebiotics 3'Sialyllactose and 6'Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis.
26144888	7	25	from	evident	1420:1426	arg1	mice					1431:1434	mice	1431:1434	mice fed milk oligosaccharides	1431:1460	These effects were not evident in mice fed milk oligosaccharides; stressor exposure did not significantly change microbial community structure in mice fed 3'SL or 6'SL.
26144888	2	26	theme	stressor-induced	682:697	arg1	behavior					712:719	stressor-induced anxiety-like behavior	682:719	stressor-induced anxiety-like behavior	682:719	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	0	27	theme	behavior	90:97	arg1	alterations					122:132	stressor-induced anxiety-like behavior and colonic microbiota alterations	60:132	stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis	60:176	The prebiotics 3'Sialyllactose and 6'Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis.
26144888	1	28	theme	gut	359:361	arg1	structure					384:392	gut microbiota community structure	359:392	gut microbiota community structure	359:392	There are extensive bidirectional interactions between the gut microbiota and the central nervous system (CNS), and studies demonstrate that stressor exposure significantly alters gut microbiota community structure.
26144888	0	29	theme	colonic	103:109	arg1	microbiota					111:120	colonic microbiota	103:120	colonic microbiota	103:120	The prebiotics 3'Sialyllactose and 6'Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis.
26144888	1	30	theme	community	374:382	arg1	structure					384:392	gut microbiota community structure	359:392	gut microbiota community structure	359:392	There are extensive bidirectional interactions between the gut microbiota and the central nervous system (CNS), and studies demonstrate that stressor exposure significantly alters gut microbiota community structure.
26144888	9	31	theme	microbial	1769:1777	arg1	communities					1779:1789	normal microbial communities	1762:1789	normal microbial communities	1762:1789	These studies indicate that milk oligosaccharides support normal microbial communities and behavioral responses during stressor exposure, potentially through effects on the gut microbiota-brain axis.
26144888	2	32	theme	human	464:468	arg1	milk					470:473	human milk	464:473	human milk	464:473	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	5	33	theme	anxiety-like	1167:1178	arg1	behavior					1180:1187	anxiety-like behavior	1167:1187	anxiety-like behavior in both the light/dark preference and open field tests in control mice	1167:1258	The stressor resulted in anxiety-like behavior in both the light/dark preference and open field tests in control mice.
26144888	4	34	theme	Stressor	984:991	arg1	exposure					993:1000	Stressor exposure	984:1000	Stressor exposure	984:1000	Stressor exposure significantly changed the structure of the colonic mucosa-associated microbiota in control mice, as indicated by changes in beta diversity.
26144888	2	35	from	alterations	617:627	arg1	composition					656:666	gut microbial community composition	632:666	gut microbial community composition	632:666	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	6	36	from	reduction	1295:1303	arg1	neurons					1317:1323	immature neurons	1308:1323	immature neurons in the dentate gyrus	1308:1344	This effect was associated with a reduction in immature neurons in the dentate gyrus as indicated by doublecortin (DCX) immunostaining.
26144888	7	37	theme	community	1520:1528	arg1	structure					1530:1538	microbial community structure	1510:1538	microbial community structure	1510:1538	These effects were not evident in mice fed milk oligosaccharides; stressor exposure did not significantly change microbial community structure in mice fed 3'SL or 6'SL.
26144888	3	38	theme	social	920:925	arg1	stressor					938:945	a social disruption stressor	918:945	a social disruption stressor	918:945	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	4	39	theme	control	1085:1091	arg1	mice					1093:1096	control mice	1085:1096	control mice	1085:1096	Stressor exposure significantly changed the structure of the colonic mucosa-associated microbiota in control mice, as indicated by changes in beta diversity.
26144888	5	40	theme	open	1227:1230	arg1	tests					1238:1242	open field tests	1227:1242	open field tests in control mice	1227:1258	The stressor resulted in anxiety-like behavior in both the light/dark preference and open field tests in control mice.
26144888	3	41	theme	control	965:971	arg1	condition					973:981	a non-stressed control condition	950:981	a non-stressed control condition	950:981	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	9	42	theme	stressor	1823:1830	arg1	exposure					1832:1839	stressor exposure	1823:1839	stressor exposure	1823:1839	These studies indicate that milk oligosaccharides support normal microbial communities and behavioral responses during stressor exposure, potentially through effects on the gut microbiota-brain axis.
26144888	4	43	theme	colonic	1045:1051	arg1	microbiota					1071:1080	the colonic mucosa-associated microbiota	1041:1080	the colonic mucosa-associated microbiota	1041:1080	Stressor exposure significantly changed the structure of the colonic mucosa-associated microbiota in control mice, as indicated by changes in beta diversity.
26144888	4	44	theme	microbiota	1071:1080	arg1	structure					1028:1036	the structure	1024:1036	the structure of the colonic mucosa-associated microbiota in control mice	1024:1096	Stressor exposure significantly changed the structure of the colonic mucosa-associated microbiota in control mice, as indicated by changes in beta diversity.
26144888	8	45	theme	3	1579:1579	arg1	SL					1590:1591	3'SL and 6'SL	1579:1591	3'SL and 6'SL	1579:1591	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
26144888	3	46	theme	standard	736:743	arg1	diet					756:759	standard laboratory diet	736:759	standard laboratory diet	736:759	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	1	47	theme	nervous	269:275	arg1	CNS					285:287	CNS	285:287	CNS	285:287	There are extensive bidirectional interactions between the gut microbiota and the central nervous system (CNS), and studies demonstrate that stressor exposure significantly alters gut microbiota community structure.
26144888	1	47	theme	nervous	269:275	arg1	system					277:282	the central nervous system	257:282	the central nervous system (CNS)	257:288	There are extensive bidirectional interactions between the gut microbiota and the central nervous system (CNS), and studies demonstrate that stressor exposure significantly alters gut microbiota community structure.
26144888	7	48	theme	milk	1440:1443	arg1	oligosaccharides					1445:1460	milk oligosaccharides	1440:1460	milk oligosaccharides	1440:1460	These effects were not evident in mice fed milk oligosaccharides; stressor exposure did not significantly change microbial community structure in mice fed 3'SL or 6'SL.
26144888	9	49	from	effects	1862:1868	arg1	axis					1898:1901	the gut microbiota-brain axis	1873:1901	the gut microbiota-brain axis	1873:1901	These studies indicate that milk oligosaccharides support normal microbial communities and behavioral responses during stressor exposure, potentially through effects on the gut microbiota-brain axis.
26144888	4	50	theme	beta	1126:1129	arg1	diversity					1131:1139	beta diversity	1126:1139	beta diversity	1126:1139	Stressor exposure significantly changed the structure of the colonic mucosa-associated microbiota in control mice, as indicated by changes in beta diversity.
26144888	0	51	from	effects	148:154	arg1	axis					173:176	the gut-brain axis	159:176	the gut-brain axis	159:176	The prebiotics 3'Sialyllactose and 6'Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis.
26144888	2	52	theme	community	646:654	arg1	composition					656:666	gut microbial community composition	632:666	gut microbial community composition	632:666	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	9	53	theme	gut	1877:1879	arg1	axis					1898:1901	the gut microbiota-brain axis	1873:1901	the gut microbiota-brain axis	1873:1901	These studies indicate that milk oligosaccharides support normal microbial communities and behavioral responses during stressor exposure, potentially through effects on the gut microbiota-brain axis.
26144888	6	54	theme	doublecortin	1362:1373	arg1	immunostaining					1381:1394	doublecortin (DCX) immunostaining	1362:1394	doublecortin (DCX) immunostaining	1362:1394	This effect was associated with a reduction in immature neurons in the dentate gyrus as indicated by doublecortin (DCX) immunostaining.
26144888	8	55	theme	anxiety-like	1637:1648	arg1	behavior					1650:1657	anxiety-like behavior	1637:1657	anxiety-like behavior	1637:1657	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
26144888	2	56	theme	gut	632:634	arg1	composition					656:666	gut microbial community composition	632:666	gut microbial community composition	632:666	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	3	57	theme	milk	802:805	arg1	Sialyllactose					852:864	6'Sialyllactose	850:864	6'Sialyllactose (6'SL)	850:871	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	57	theme	milk	802:805	arg1	oligosaccharides					807:822	the human milk oligosaccharides	792:822	the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL)	792:871	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	57	theme	milk	802:805	arg1	Sialyllactose					826:838	3'Sialyllactose	824:838	3'Sialyllactose (3'SL)	824:845	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	6	58	theme	dentate	1332:1338	arg1	gyrus					1340:1344	the dentate gyrus	1328:1344	the dentate gyrus	1328:1344	This effect was associated with a reduction in immature neurons in the dentate gyrus as indicated by doublecortin (DCX) immunostaining.
26144888	1	59	theme	extensive	189:197	arg1	interactions					213:224	extensive bidirectional interactions	189:224	extensive bidirectional interactions between the gut microbiota	189:251	There are extensive bidirectional interactions between the gut microbiota and the central nervous system (CNS), and studies demonstrate that stressor exposure significantly alters gut microbiota community structure.
26144888	2	60	located	found	440:444	arg1	levels					454:459	high levels	449:459	high levels in human milk	449:473	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	2	60	located	found	440:444	arg2	oligosaccharides					413:428	oligosaccharides	413:428	oligosaccharides	413:428	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	8	61	theme	numbers	1670:1676	arg1	tests					1628:1632	tests	1628:1632	tests of anxiety-like behavior and normal numbers of DCX+ immature neurons	1628:1701	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
26144888	6	62	theme	immature	1308:1315	arg1	neurons					1317:1323	immature neurons	1308:1323	immature neurons in the dentate gyrus	1308:1344	This effect was associated with a reduction in immature neurons in the dentate gyrus as indicated by doublecortin (DCX) immunostaining.
26144888	8	63	theme	DCX+	1681:1684	arg1	neurons					1695:1701	DCX+ immature neurons	1681:1701	DCX+ immature neurons	1681:1701	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
26144888	5	64	from	preference	1212:1221	arg1	mice					1255:1258	control mice	1247:1258	control mice	1247:1258	The stressor resulted in anxiety-like behavior in both the light/dark preference and open field tests in control mice.
26144888	0	65	theme	gut-brain	163:171	arg1	axis					173:176	the gut-brain axis	159:176	the gut-brain axis	159:176	The prebiotics 3'Sialyllactose and 6'Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis.
26144888	8	66	theme	neurons	1695:1701	arg1	behavior					1650:1657	anxiety-like behavior	1637:1657	anxiety-like behavior	1637:1657	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
26144888	8	66	theme	neurons	1695:1701	arg1	numbers					1670:1676	normal numbers	1663:1676	normal numbers of DCX+ immature neurons	1663:1701	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
26144888	2	67	theme	commensal	566:574	arg1	microbes					576:583	beneficial commensal microbes	555:583	beneficial commensal microbes	555:583	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	4	68	from	changes	1115:1121	arg1	diversity					1131:1139	beta diversity	1126:1139	beta diversity	1126:1139	Stressor exposure significantly changed the structure of the colonic mucosa-associated microbiota in control mice, as indicated by changes in beta diversity.
26144888	2	69	from	levels	454:459	arg1	milk					470:473	human milk	464:473	human milk	464:473	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	5	70	theme	control	1247:1253	arg1	mice					1255:1258	control mice	1247:1258	control mice	1247:1258	The stressor resulted in anxiety-like behavior in both the light/dark preference and open field tests in control mice.
26144888	0	71	theme	anxiety-like	77:88	arg1	behavior					90:97	stressor-induced anxiety-like behavior	60:97	stressor-induced anxiety-like behavior	60:97	The prebiotics 3'Sialyllactose and 6'Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis.
26144888	3	72	dep	oligosaccharides	807:822	arg1	Sialyllactose					852:864	6'Sialyllactose	850:864	6'Sialyllactose (6'SL)	850:871	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	72	dep	oligosaccharides	807:822	arg1	SL					843:844	3'SL	841:844	3'SL	841:844	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	72	dep	oligosaccharides	807:822	arg1	oligosaccharides					807:822	the human milk oligosaccharides	792:822	the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL)	792:871	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	72	dep	oligosaccharides	807:822	arg1	SL					869:870	6'SL	867:870	6'SL	867:870	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	72	dep	oligosaccharides	807:822	arg1	Sialyllactose					826:838	3'Sialyllactose	824:838	3'Sialyllactose (3'SL)	824:845	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	2	73	theme	anxiety-like	699:710	arg1	behavior					712:719	stressor-induced anxiety-like behavior	682:719	stressor-induced anxiety-like behavior	682:719	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	8	74	dep	3	1579:1579	arg1	6					1588:1588	6	1588:1588	6	1588:1588	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
26144888	8	74	dep	3	1579:1579	arg1	SL					1581:1582	SL	1581:1582	SL	1581:1582	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
26144888	0	75	theme	microbiota	111:120	arg1	alterations					122:132	stressor-induced anxiety-like behavior and colonic microbiota alterations	60:132	stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis	60:176	The prebiotics 3'Sialyllactose and 6'Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis.
26144888	1	76	theme	microbiota	363:372	arg1	structure					384:392	gut microbiota community structure	359:392	gut microbiota community structure	359:392	There are extensive bidirectional interactions between the gut microbiota and the central nervous system (CNS), and studies demonstrate that stressor exposure significantly alters gut microbiota community structure.
26144888	2	77	theme	brain	511:515	arg1	development					517:527	impact brain development	504:527	impact brain development	504:527	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	1	78	theme	gut	238:240	arg1	microbiota					242:251	the gut microbiota	234:251	the gut microbiota	234:251	There are extensive bidirectional interactions between the gut microbiota and the central nervous system (CNS), and studies demonstrate that stressor exposure significantly alters gut microbiota community structure.
26144888	3	79	theme	prior	885:889	arg1	weeks					879:883	2 weeks	877:883	2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition	877:981	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	80	contain	containing	781:790	arg2	Sialyllactose					826:838	3'Sialyllactose	824:838	3'Sialyllactose (3'SL)	824:845	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	80	contain	containing	781:790	arg2	Sialyllactose					852:864	6'Sialyllactose	850:864	6'Sialyllactose (6'SL)	850:871	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	80	contain	containing	781:790	arg1	diet					756:759	standard laboratory diet	736:759	standard laboratory diet	736:759	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	3	80	contain	containing	781:790	arg2	oligosaccharides					807:822	the human milk oligosaccharides	792:822	the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL)	792:871	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	6	81	from	neurons	1317:1323	arg1	gyrus					1340:1344	the dentate gyrus	1328:1344	the dentate gyrus	1328:1344	This effect was associated with a reduction in immature neurons in the dentate gyrus as indicated by doublecortin (DCX) immunostaining.
26144888	2	82	theme	high	449:452	arg1	levels					454:459	high levels	449:459	high levels in human milk	449:473	We tested whether oligosaccharides naturally found in high levels in human milk, which have been reported to impact brain development and enhance the growth of beneficial commensal microbes, would prevent stressor-induced alterations in gut microbial community composition and attenuate stressor-induced anxiety-like behavior.
26144888	9	83	theme	behavioral	1795:1804	arg1	responses					1806:1814	behavioral responses	1795:1814	behavioral responses	1795:1814	These studies indicate that milk oligosaccharides support normal microbial communities and behavioral responses during stressor exposure, potentially through effects on the gut microbiota-brain axis.
26144888	7	84	theme	microbial	1510:1518	arg1	structure					1530:1538	microbial community structure	1510:1538	microbial community structure	1510:1538	These effects were not evident in mice fed milk oligosaccharides; stressor exposure did not significantly change microbial community structure in mice fed 3'SL or 6'SL.
26144888	3	85	theme	disruption	927:936	arg1	stressor					938:945	a social disruption stressor	918:945	a social disruption stressor	918:945	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	5	86	theme	field	1232:1236	arg1	tests					1238:1242	open field tests	1227:1242	open field tests in control mice	1227:1258	The stressor resulted in anxiety-like behavior in both the light/dark preference and open field tests in control mice.
26144888	0	87	dep	prebiotics	4:13	arg1	Sialyllactose					37:49	6'Sialyllactose	35:49	6'Sialyllactose	35:49	The prebiotics 3'Sialyllactose and 6'Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis.
26144888	0	87	dep	prebiotics	4:13	arg1	prebiotics					4:13	The prebiotics 3'Sialyllactose and 6'Sialyllactose	0:49	The prebiotics 3'Sialyllactose and 6'Sialyllactose	0:49	The prebiotics 3'Sialyllactose and 6'Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis.
26144888	0	87	dep	prebiotics	4:13	arg1	Sialyllactose					17:29	3'Sialyllactose	15:29	3'Sialyllactose	15:29	The prebiotics 3'Sialyllactose and 6'Sialyllactose diminish stressor-induced anxiety-like behavior and colonic microbiota alterations: Evidence for effects on the gut-brain axis.
26144888	3	88	theme	laboratory	745:754	arg1	diet					756:759	standard laboratory diet	736:759	standard laboratory diet	736:759	Mice were fed standard laboratory diet, or laboratory diet containing the human milk oligosaccharides 3'Sialyllactose (3'SL) or 6'Sialyllactose (6'SL) for 2 weeks prior to being exposed to either a social disruption stressor or a non-stressed control condition.
26144888	9	89	theme	milk	1732:1735	arg1	oligosaccharides					1737:1752	milk oligosaccharides	1732:1752	milk oligosaccharides	1732:1752	These studies indicate that milk oligosaccharides support normal microbial communities and behavioral responses during stressor exposure, potentially through effects on the gut microbiota-brain axis.
26144888	8	90	theme	normal	1609:1614	arg1	behavior					1616:1623	normal behavior	1609:1623	normal behavior	1609:1623	In addition, 3'SL and 6'SL helped maintain normal behavior on tests of anxiety-like behavior and normal numbers of DCX+ immature neurons.
27287172	7	0	theme	high	1098:1101	arg1	stability					1103:1111	high stability	1098:1111	high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively	1098:1221	These suspensions had high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively, as well as nanoparticle diameters of about 140nm.
27287172	4	1	theme	concentration	651:663	arg1	combinations					621:632	different combinations	611:632	different combinations of chitosan (CH) concentration, CH:TPP ratio and GA	611:684	The nanoparticles were prepared by ionotropic gelation using tripolyphosphate (TPP), at different combinations of chitosan (CH) concentration, CH:TPP ratio and GA.
27287172	5	2	theme	5	805:805	arg1	%					780:780	CH 0.76%	773:780	CH 0.76% (w/w)	773:786	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	2	theme	5	805:805	arg1	%					851:851	82%	849:851	82% of PEE	849:858	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	2	theme	5	805:805	arg1	w/w					783:785	w/w	783:785	w/w	783:785	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	2	theme	5	805:805	arg1	ratio					796:800	CH:TPP ratio	789:800	CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV	789:843	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	2	theme	5	805:805	arg1	PEE					856:858	PEE	856:858	PEE	856:858	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	2	theme	5	805:805	arg1	5					805:805	5	805:805	5	805:805	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	2	theme	5	805:805	arg1	37mgGA/gCH					811:820	37mgGA/gCH	811:820	37mgGA/gCH	811:820	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	3	3	theme	chitosan-based	335:348	arg1	nanoparticles					350:362	chitosan-based nanoparticles	335:362	chitosan-based nanoparticles functionalized with gallic acid (GA)	335:399	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	4	4	theme	ionotropic	558:567	arg1	gelation					569:576	ionotropic gelation	558:576	ionotropic gelation using tripolyphosphate (TPP)	558:605	The nanoparticles were prepared by ionotropic gelation using tripolyphosphate (TPP), at different combinations of chitosan (CH) concentration, CH:TPP ratio and GA.
27287172	3	5	theme	nanoparticles	350:362	arg1	parameters					321:330	The design parameters	310:330	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA)	310:399	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	6	6	theme	chitosan	1003:1010	arg1	concentrations					985:998	concentrations	985:998	concentrations of chitosan ranging between 0.5 and 0.75%	985:1040	Analysis through QuickScan and turbidity demonstrated that the most stable nanoparticle suspensions were achieved combining concentrations of chitosan ranging between 0.5 and 0.75% with CH:TPP ratios higher than 3.
27287172	1	7	theme	Active	72:77	arg1	nanoparticles					79:91	Active nanoparticles	72:91	Active nanoparticles based on chitosan	72:109	Active nanoparticles based on chitosan could be applied as a support for the modulation of gallic acid delivery.
27287172	1	7	theme	Active	72:77	arg1	support					133:139	a support	131:139	a support for the modulation of gallic acid delivery	131:182	Active nanoparticles based on chitosan could be applied as a support for the modulation of gallic acid delivery.
27287172	3	8	theme	potential	461:469	arg1	analysis					444:451	the analysis	440:451	the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE)	440:520	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	5	9	theme	+50mV	839:843	arg1	ZP					833:834	ZP	833:834	ZP of +50mV	833:843	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	6	10	theme	nanoparticle	936:947	arg1	suspensions					949:959	the most stable nanoparticle suspensions	920:959	the most stable nanoparticle suspensions	920:959	Analysis through QuickScan and turbidity demonstrated that the most stable nanoparticle suspensions were achieved combining concentrations of chitosan ranging between 0.5 and 0.75% with CH:TPP ratios higher than 3.
27287172	3	11	theme	encapsulation	491:503	arg1	PEE					517:519	PEE	517:519	PEE	517:519	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	3	11	theme	encapsulation	491:503	arg1	efficiency					505:514	percentage encapsulation efficiency	480:514	percentage encapsulation efficiency (PEE)	480:520	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	1	12	theme	delivery	175:182	arg1	modulation					149:158	the modulation	145:158	the modulation of gallic acid delivery	145:182	Active nanoparticles based on chitosan could be applied as a support for the modulation of gallic acid delivery.
27287172	1	13	theme	gallic	163:168	arg1	delivery					175:182	gallic acid delivery	163:182	gallic acid delivery	163:182	Active nanoparticles based on chitosan could be applied as a support for the modulation of gallic acid delivery.
27287172	3	14	theme	efficiency	505:514	arg1	analysis					444:451	the analysis	440:451	the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE)	440:520	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	1	15	theme	acid	170:173	arg1	delivery					175:182	gallic acid delivery	163:182	gallic acid delivery	163:182	Active nanoparticles based on chitosan could be applied as a support for the modulation of gallic acid delivery.
27287172	3	16	theme	gallic	384:389	arg1	GA					397:398	GA	397:398	GA	397:398	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	3	16	theme	gallic	384:389	arg1	acid					391:394	gallic acid	384:394	gallic acid (GA)	384:399	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	6	17	theme	TPP	1050:1052	arg1	ratios					1054:1059	CH:TPP ratios	1047:1059	CH:TPP ratios higher than 3	1047:1073	Analysis through QuickScan and turbidity demonstrated that the most stable nanoparticle suspensions were achieved combining concentrations of chitosan ranging between 0.5 and 0.75% with CH:TPP ratios higher than 3.
27287172	2	18	theme	pharmaceutical	288:301	arg1	areas					303:307	pharmaceutical areas	288:307	pharmaceutical areas	288:307	In this sense, these nanostructures could be employed in different fields such as food, packaging, and pharmaceutical areas.
27287172	4	19	theme	chitosan	637:644	arg1	concentration					651:663	chitosan (CH) concentration	637:663	chitosan (CH) concentration	637:663	The nanoparticles were prepared by ionotropic gelation using tripolyphosphate (TPP), at different combinations of chitosan (CH) concentration, CH:TPP ratio and GA.
27287172	4	20	theme	ratio	673:677	arg1	combinations					621:632	different combinations	611:632	different combinations of chitosan (CH) concentration, CH:TPP ratio and GA	611:684	The nanoparticles were prepared by ionotropic gelation using tripolyphosphate (TPP), at different combinations of chitosan (CH) concentration, CH:TPP ratio and GA.
27287172	5	21	theme	TPP	792:794	arg1	ratio					796:800	CH:TPP ratio	789:800	CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV	789:843	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	0	22	theme	nanoparticles	25:37	arg1	Design					0:5	Design	0:5	Design of chitosan-based nanoparticles	0:37	Design of chitosan-based nanoparticles functionalized with gallic acid.
27287172	8	23	theme	stability	1417:1425	arg1	occurrence					1292:1301	the occurrence	1288:1301	the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation	1288:1388	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	8	23	theme	stability	1417:1425	arg1	improvement					1398:1408	the improvement	1394:1408	the improvement of the stability of the active agent	1394:1445	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	6	24	theme	CH	1047:1048	arg1	ratios					1054:1059	CH:TPP ratios	1047:1059	CH:TPP ratios higher than 3	1047:1073	Analysis through QuickScan and turbidity demonstrated that the most stable nanoparticle suspensions were achieved combining concentrations of chitosan ranging between 0.5 and 0.75% with CH:TPP ratios higher than 3.
27287172	0	25	theme	chitosan-based	10:23	arg1	nanoparticles					25:37	chitosan-based nanoparticles	10:37	chitosan-based nanoparticles	10:37	Design of chitosan-based nanoparticles functionalized with gallic acid.
27287172	8	26	theme	active	1434:1439	arg1	agent					1441:1445	the active agent	1430:1445	the active agent	1430:1445	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	5	27	theme	PEE	856:858	arg1	%					780:780	CH 0.76%	773:780	CH 0.76% (w/w)	773:786	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	27	theme	PEE	856:858	arg1	%					851:851	82%	849:851	82% of PEE	849:858	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	27	theme	PEE	856:858	arg1	w/w					783:785	w/w	783:785	w/w	783:785	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	27	theme	PEE	856:858	arg1	ratio					796:800	CH:TPP ratio	789:800	CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV	789:843	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	27	theme	PEE	856:858	arg1	PEE					856:858	PEE	856:858	PEE	856:858	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	27	theme	PEE	856:858	arg1	5					805:805	5	805:805	5	805:805	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	27	theme	PEE	856:858	arg1	37mgGA/gCH					811:820	37mgGA/gCH	811:820	37mgGA/gCH	811:820	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	8	28	theme	bond	1320:1323	arg1	occurrence					1292:1301	the occurrence	1288:1301	the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation	1288:1388	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	8	28	theme	bond	1320:1323	arg1	improvement					1398:1408	the improvement	1394:1408	the improvement of the stability of the active agent	1394:1445	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	4	29	theme	GA	683:684	arg1	combinations					621:632	different combinations	611:632	different combinations of chitosan (CH) concentration, CH:TPP ratio and GA	611:684	The nanoparticles were prepared by ionotropic gelation using tripolyphosphate (TPP), at different combinations of chitosan (CH) concentration, CH:TPP ratio and GA.
27287172	5	30	theme	CH	789:790	arg1	ratio					796:800	CH:TPP ratio	789:800	CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV	789:843	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	8	31	theme	hydrogen	1311:1318	arg1	bond					1320:1323	hydrogen bond	1311:1323	hydrogen bond	1311:1323	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	6	32	theme	stable	929:934	arg1	suspensions					949:959	the most stable nanoparticle suspensions	920:959	the most stable nanoparticle suspensions	920:959	Analysis through QuickScan and turbidity demonstrated that the most stable nanoparticle suspensions were achieved combining concentrations of chitosan ranging between 0.5 and 0.75% with CH:TPP ratios higher than 3.
27287172	7	33	dep	means	1126:1130	arg1	ZP					1132:1133	ZP	1132:1133	ZP	1132:1133	These suspensions had high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively, as well as nanoparticle diameters of about 140nm.
27287172	7	33	dep	means	1126:1130	arg1	values					1153:1158	transmittance values	1139:1158	transmittance values	1139:1158	These suspensions had high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively, as well as nanoparticle diameters of about 140nm.
27287172	5	34	theme	CH	773:774	arg1	5					805:805	5	805:805	5	805:805	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	34	theme	CH	773:774	arg1	w/w					783:785	w/w	783:785	w/w	783:785	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	34	theme	CH	773:774	arg1	%					780:780	CH 0.76%	773:780	CH 0.76% (w/w)	773:786	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	34	theme	CH	773:774	arg1	PEE					856:858	PEE	856:858	PEE	856:858	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	34	theme	CH	773:774	arg1	37mgGA/gCH					811:820	37mgGA/gCH	811:820	37mgGA/gCH	811:820	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	6	35	theme	most	924:927	arg1	suspensions					949:959	the most stable nanoparticle suspensions	920:959	the most stable nanoparticle suspensions	920:959	Analysis through QuickScan and turbidity demonstrated that the most stable nanoparticle suspensions were achieved combining concentrations of chitosan ranging between 0.5 and 0.75% with CH:TPP ratios higher than 3.
27287172	5	36	theme	optimum	739:745	arg1	formulation					747:757	the optimum formulation	735:757	the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE	735:858	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	8	37	theme	agent	1441:1445	arg1	stability					1417:1425	the stability	1413:1425	the stability of the active agent	1413:1445	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	5	38	theme	desirability	694:705	arg1	methodology					707:717	Global desirability methodology	687:717	Global desirability methodology	687:717	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	7	39	theme	nanoparticle	1235:1246	arg1	diameters					1248:1256	nanoparticle diameters	1235:1256	nanoparticle diameters of about 140nm	1235:1271	These suspensions had high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively, as well as nanoparticle diameters of about 140nm.
27287172	4	40	theme	CH	666:667	arg1	ratio					673:677	CH:TPP ratio	666:677	CH:TPP ratio	666:677	The nanoparticles were prepared by ionotropic gelation using tripolyphosphate (TPP), at different combinations of chitosan (CH) concentration, CH:TPP ratio and GA.
27287172	0	41	theme	gallic	59:64	arg1	acid					66:69	gallic acid	59:69	gallic acid	59:69	Design of chitosan-based nanoparticles functionalized with gallic acid.
27287172	8	42	theme	interactions	1335:1346	arg1	occurrence					1292:1301	the occurrence	1288:1301	the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation	1288:1388	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	8	42	theme	interactions	1335:1346	arg1	improvement					1398:1408	the improvement	1394:1408	the improvement of the stability of the active agent	1394:1445	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	7	43	contain	had	1094:1096	arg1	suspensions					1082:1092	These suspensions	1076:1092	These suspensions	1076:1092	These suspensions had high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively, as well as nanoparticle diameters of about 140nm.
27287172	7	43	contain	had	1094:1096	arg2	stability					1103:1111	high stability	1098:1111	high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively	1098:1221	These suspensions had high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively, as well as nanoparticle diameters of about 140nm.
27287172	7	43	contain	had	1094:1096	arg2	diameters					1248:1256	nanoparticle diameters	1235:1256	nanoparticle diameters of about 140nm	1235:1271	These suspensions had high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively, as well as nanoparticle diameters of about 140nm.
27287172	8	44	theme	ionic	1329:1333	arg1	interactions					1335:1346	ionic interactions	1329:1346	ionic interactions of CH-TPP which allowed the encapsulation	1329:1388	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	7	45	theme	transmittance	1139:1151	arg1	values					1153:1158	transmittance values	1139:1158	transmittance values	1139:1158	These suspensions had high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively, as well as nanoparticle diameters of about 140nm.
27287172	6	46	theme	higher	1061:1066	arg1	ratios					1054:1059	CH:TPP ratios	1047:1059	CH:TPP ratios higher than 3	1047:1073	Analysis through QuickScan and turbidity demonstrated that the most stable nanoparticle suspensions were achieved combining concentrations of chitosan ranging between 0.5 and 0.75% with CH:TPP ratios higher than 3.
27287172	4	47	theme	TPP	669:671	arg1	ratio					673:677	CH:TPP ratio	666:677	CH:TPP ratio	666:677	The nanoparticles were prepared by ionotropic gelation using tripolyphosphate (TPP), at different combinations of chitosan (CH) concentration, CH:TPP ratio and GA.
27287172	7	48	theme	140nm	1267:1271	arg1	stability					1103:1111	high stability	1098:1111	high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively	1098:1221	These suspensions had high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively, as well as nanoparticle diameters of about 140nm.
27287172	7	48	theme	140nm	1267:1271	arg1	diameters					1248:1256	nanoparticle diameters	1235:1256	nanoparticle diameters of about 140nm	1235:1271	These suspensions had high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively, as well as nanoparticle diameters of about 140nm.
27287172	5	49	theme	37mgGA/gCH	811:820	arg1	%					780:780	CH 0.76%	773:780	CH 0.76% (w/w)	773:786	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	49	theme	37mgGA/gCH	811:820	arg1	%					851:851	82%	849:851	82% of PEE	849:858	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	49	theme	37mgGA/gCH	811:820	arg1	w/w					783:785	w/w	783:785	w/w	783:785	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	49	theme	37mgGA/gCH	811:820	arg1	ratio					796:800	CH:TPP ratio	789:800	CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV	789:843	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	49	theme	37mgGA/gCH	811:820	arg1	PEE					856:858	PEE	856:858	PEE	856:858	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	49	theme	37mgGA/gCH	811:820	arg1	5					805:805	5	805:805	5	805:805	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	5	49	theme	37mgGA/gCH	811:820	arg1	37mgGA/gCH					811:820	37mgGA/gCH	811:820	37mgGA/gCH	811:820	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	2	50	theme	different	242:250	arg1	food					267:270	food	267:270	food	267:270	In this sense, these nanostructures could be employed in different fields such as food, packaging, and pharmaceutical areas.
27287172	2	50	theme	different	242:250	arg1	areas					303:307	pharmaceutical areas	288:307	pharmaceutical areas	288:307	In this sense, these nanostructures could be employed in different fields such as food, packaging, and pharmaceutical areas.
27287172	2	50	theme	different	242:250	arg1	packaging					273:281	packaging	273:281	packaging	273:281	In this sense, these nanostructures could be employed in different fields such as food, packaging, and pharmaceutical areas.
27287172	2	50	theme	different	242:250	arg1	fields					252:257	different fields	242:257	different fields such as food, packaging, and pharmaceutical areas	242:307	In this sense, these nanostructures could be employed in different fields such as food, packaging, and pharmaceutical areas.
27287172	3	51	theme	zeta	456:459	arg1	potential					461:469	zeta potential	456:469	zeta potential (ZP)	456:474	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	3	51	theme	zeta	456:459	arg1	ZP					472:473	ZP	472:473	ZP	472:473	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	5	52	theme	Global	687:692	arg1	methodology					707:717	Global desirability methodology	687:717	Global desirability methodology	687:717	Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w/w), CH:TPP ratio of 5 and 37mgGA/gCH leading to ZP of +50mV and 82% of PEE.
27287172	8	53	theme	CH-TPP	1351:1356	arg1	bond					1320:1323	hydrogen bond	1311:1323	hydrogen bond	1311:1323	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	8	53	theme	CH-TPP	1351:1356	arg1	interactions					1335:1346	ionic interactions	1329:1346	ionic interactions of CH-TPP which allowed the encapsulation	1329:1388	FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.
27287172	4	54	theme	different	611:619	arg1	combinations					621:632	different combinations	611:632	different combinations of chitosan (CH) concentration, CH:TPP ratio and GA	611:684	The nanoparticles were prepared by ionotropic gelation using tripolyphosphate (TPP), at different combinations of chitosan (CH) concentration, CH:TPP ratio and GA.
27287172	3	55	theme	design	314:319	arg1	parameters					321:330	The design parameters	310:330	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA)	310:399	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	3	56	dep	analysis	444:451	arg1	means					431:435	means	431:435	means	431:435	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	3	57	theme	percentage	480:489	arg1	PEE					517:519	PEE	517:519	PEE	517:519	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
27287172	3	57	theme	percentage	480:489	arg1	efficiency					505:514	percentage encapsulation efficiency	480:514	percentage encapsulation efficiency (PEE)	480:520	The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE).
24519746	0	0	theme	active	75:80	arg1	matrix					68:73	a carbon matrix	59:73	a carbon matrix active for the oxidation of aqueous contaminants with H₂O₂	59:132	Controlled formation of reactive Fe particles dispersed in a carbon matrix active for the oxidation of aqueous contaminants with H₂O₂.
24519746	6	1	theme	Fe	1166:1167	arg1	species					1181:1187	Fe(2+) surface species	1166:1187	Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction	1166:1245	These results are discussed in terms of Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction.
24519746	6	2	from	nanoparticles	1202:1214	arg1	terms					1157:1161	terms	1157:1161	terms of Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction	1157:1245	These results are discussed in terms of Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction.
24519746	6	3	theme	Fenton	1231:1236	arg1	reaction					1238:1245	the Fenton reaction	1227:1245	the Fenton reaction	1227:1245	These results are discussed in terms of Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction.
24519746	4	4	from	temperatures	829:840	arg1	treatments					808:817	treatments	808:817	treatments	808:817	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	1	5	from	decomposition	246:258	arg1	sucrose					278:284	sucrose	278:284	sucrose	278:284	In this work, reactive iron nanoparticles dispersed in a carbon matrix were produced by the controlled thermal decomposition of Fe(3+) ions in sucrose.
24519746	4	6	dep	°C	861:862	arg1	i.e.					843:846	i.e.	843:846	i.e.	843:846	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	7	theme	Mössbauer	556:564	arg1	Analyses					525:532	400, 600, and 800 °C. Analyses	503:532	400, 600, and 800 °C. Analyses by X-ray diffraction	503:553	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	7	theme	Mössbauer	556:564	arg1	spectroscopy					566:577	Mössbauer spectroscopy	556:577	Mössbauer spectroscopy	556:577	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	1	8	theme	controlled	227:236	arg1	decomposition					246:258	the controlled thermal decomposition	223:258	the controlled thermal decomposition of Fe(3+) ions in sucrose	223:284	In this work, reactive iron nanoparticles dispersed in a carbon matrix were produced by the controlled thermal decomposition of Fe(3+) ions in sucrose.
24519746	4	9	theme	Fe3O4	785:789	arg1	particles					791:799	Fe3O4 particles	785:799	Fe3O4 particles	785:799	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	5	10	theme	methylene	953:961	arg1	oxidation					940:948	the oxidation	936:948	the oxidation of methylene blue with H2O2	936:976	The composites were tested for the oxidation of methylene blue with H2O2 by a Fenton-type reaction and also H2O2 decomposition, showing better performance for the material containing 8 % of iron heated at 400 and 600 °C.
24519746	5	11	with	oxidation	940:948	arg1	H2O2					973:976	H2O2	973:976	H2O2	973:976	The composites were tested for the oxidation of methylene blue with H2O2 by a Fenton-type reaction and also H2O2 decomposition, showing better performance for the material containing 8 % of iron heated at 400 and 600 °C.
24519746	1	12	theme	thermal	238:244	arg1	decomposition					246:258	the controlled thermal decomposition	223:258	the controlled thermal decomposition of Fe(3+) ions in sucrose	223:284	In this work, reactive iron nanoparticles dispersed in a carbon matrix were produced by the controlled thermal decomposition of Fe(3+) ions in sucrose.
24519746	4	13	theme	magnetization	580:592	arg1	Analyses					525:532	400, 600, and 800 °C. Analyses	503:532	400, 600, and 800 °C. Analyses by X-ray diffraction	503:553	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	13	theme	magnetization	580:592	arg1	measurements					594:605	magnetization measurements	580:605	magnetization measurements	580:605	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	14	theme	surface	659:665	arg1	area					667:670	heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area	493:670	heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area	493:670	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	15	theme	X-ray	537:541	arg1	diffraction					543:553	X-ray diffraction	537:553	X-ray diffraction	537:553	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	16	theme	higher	822:827	arg1	temperatures					829:840	higher temperatures	822:840	higher temperatures	822:840	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	6	17	from	terms	1157:1161	arg1	nanoparticles					1202:1214	the Fe3O4 nanoparticles	1192:1214	the Fe3O4 nanoparticles active for the Fenton reaction	1192:1245	These results are discussed in terms of Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction.
24519746	2	18	theme	carbonaceous	401:412	arg1	matrix					414:419	a porous carbonaceous matrix	392:419	a porous carbonaceous matrix	392:419	During the sucrose decomposition, the Fe(3+) ions are reduced to form iron nanometric cores dispersed in a porous carbonaceous matrix.
24519746	5	19	theme	better	1041:1046	arg1	performance					1048:1058	better performance	1041:1058	better performance for the material containing 8 % of iron heated at 400 and 600 °C	1041:1123	The composites were tested for the oxidation of methylene blue with H2O2 by a Fenton-type reaction and also H2O2 decomposition, showing better performance for the material containing 8 % of iron heated at 400 and 600 °C.
24519746	4	20	from	Analyses	525:532	arg1	heated					493:498	heated	493:498	heated	493:498	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	1	21	theme	reactive	149:156	arg1	nanoparticles					163:175	reactive iron nanoparticles	149:175	reactive iron nanoparticles dispersed in a carbon matrix	149:204	In this work, reactive iron nanoparticles dispersed in a carbon matrix were produced by the controlled thermal decomposition of Fe(3+) ions in sucrose.
24519746	6	22	theme	active	1216:1221	arg1	nanoparticles					1202:1214	the Fe3O4 nanoparticles	1192:1214	the Fe3O4 nanoparticles active for the Fenton reaction	1192:1245	These results are discussed in terms of Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction.
24519746	1	23	theme	iron	158:161	arg1	nanoparticles					163:175	reactive iron nanoparticles	149:175	reactive iron nanoparticles dispersed in a carbon matrix	149:204	In this work, reactive iron nanoparticles dispersed in a carbon matrix were produced by the controlled thermal decomposition of Fe(3+) ions in sucrose.
24519746	0	24	theme	Controlled	0:9	arg1	formation					11:19	Controlled formation	0:19	Controlled formation of reactive Fe particles	0:44	Controlled formation of reactive Fe particles dispersed in a carbon matrix active for the oxidation of aqueous contaminants with H₂O₂.
24519746	6	25	from	species	1181:1187	arg1	nanoparticles					1202:1214	the Fe3O4 nanoparticles	1192:1214	the Fe3O4 nanoparticles active for the Fenton reaction	1192:1245	These results are discussed in terms of Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction.
24519746	4	26	theme	Raman	608:612	arg1	Analyses					525:532	400, 600, and 800 °C. Analyses	503:532	400, 600, and 800 °C. Analyses by X-ray diffraction	503:553	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	26	theme	Raman	608:612	arg1	spectroscopy					614:625	Raman spectroscopy	608:625	Raman spectroscopy	608:625	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	2	27	theme	nanometric	362:371	arg1	cores					373:377	iron nanometric cores	357:377	iron nanometric cores dispersed in a porous carbonaceous matrix	357:419	During the sucrose decomposition, the Fe(3+) ions are reduced to form iron nanometric cores dispersed in a porous carbonaceous matrix.
24519746	1	28	theme	Fe	263:264	arg1	ions					270:273	Fe(3+) ions	263:273	Fe(3+) ions	263:273	In this work, reactive iron nanoparticles dispersed in a carbon matrix were produced by the controlled thermal decomposition of Fe(3+) ions in sucrose.
24519746	0	29	theme	contaminants	111:122	arg1	oxidation					90:98	the oxidation	86:98	the oxidation of aqueous contaminants with H₂O₂	86:132	Controlled formation of reactive Fe particles dispersed in a carbon matrix active for the oxidation of aqueous contaminants with H₂O₂.
24519746	4	30	theme	termogravimetric	628:643	arg1	Analyses					525:532	400, 600, and 800 °C. Analyses	503:532	400, 600, and 800 °C. Analyses by X-ray diffraction	503:553	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	30	theme	termogravimetric	628:643	arg1	analyses					645:652	termogravimetric analyses	628:652	termogravimetric analyses	628:652	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	2	31	theme	iron	357:360	arg1	cores					373:377	iron nanometric cores	357:377	iron nanometric cores dispersed in a porous carbonaceous matrix	357:419	During the sucrose decomposition, the Fe(3+) ions are reduced to form iron nanometric cores dispersed in a porous carbonaceous matrix.
24519746	0	32	theme	aqueous	103:109	arg1	contaminants					111:122	aqueous contaminants	103:122	aqueous contaminants with H₂O₂	103:132	Controlled formation of reactive Fe particles dispersed in a carbon matrix active for the oxidation of aqueous contaminants with H₂O₂.
24519746	6	33	theme	species	1181:1187	arg1	terms					1157:1161	terms	1157:1161	terms of Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction	1157:1245	These results are discussed in terms of Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction.
24519746	0	34	theme	particles	36:44	arg1	formation					11:19	Controlled formation	0:19	Controlled formation of reactive Fe particles	0:44	Controlled formation of reactive Fe particles dispersed in a carbon matrix active for the oxidation of aqueous contaminants with H₂O₂.
24519746	4	35	theme	transmission	687:698	arg1	microscopy					709:718	transmission electron microscopy	687:718	transmission electron microscopy	687:718	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	5	36	theme	H2O2	1013:1016	arg1	decomposition					1018:1030	H2O2 decomposition	1013:1030	H2O2 decomposition	1013:1030	The composites were tested for the oxidation of methylene blue with H2O2 by a Fenton-type reaction and also H2O2 decomposition, showing better performance for the material containing 8 % of iron heated at 400 and 600 °C.
24519746	0	37	theme	Fe	33:34	arg1	particles					36:44	reactive Fe particles	24:44	reactive Fe particles	24:44	Controlled formation of reactive Fe particles dispersed in a carbon matrix active for the oxidation of aqueous contaminants with H₂O₂.
24519746	5	38	theme	blue	963:966	arg1	methylene					953:961	methylene blue	953:966	methylene blue	953:966	The composites were tested for the oxidation of methylene blue with H2O2 by a Fenton-type reaction and also H2O2 decomposition, showing better performance for the material containing 8 % of iron heated at 400 and 600 °C.
24519746	2	39	theme	porous	394:399	arg1	matrix					414:419	a porous carbonaceous matrix	392:419	a porous carbonaceous matrix	392:419	During the sucrose decomposition, the Fe(3+) ions are reduced to form iron nanometric cores dispersed in a porous carbonaceous matrix.
24519746	0	40	theme	reactive	24:31	arg1	particles					36:44	reactive Fe particles	24:44	reactive Fe particles	24:44	Controlled formation of reactive Fe particles dispersed in a carbon matrix active for the oxidation of aqueous contaminants with H₂O₂.
24519746	1	41	theme	carbon	192:197	arg1	matrix					199:204	a carbon matrix	190:204	a carbon matrix	190:204	In this work, reactive iron nanoparticles dispersed in a carbon matrix were produced by the controlled thermal decomposition of Fe(3+) ions in sucrose.
24519746	6	42	theme	surface	1173:1179	arg1	species					1181:1187	Fe(2+) surface species	1166:1187	Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction	1166:1245	These results are discussed in terms of Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction.
24519746	1	43	theme	ions	270:273	arg1	decomposition					246:258	the controlled thermal decomposition	223:258	the controlled thermal decomposition of Fe(3+) ions in sucrose	223:284	In this work, reactive iron nanoparticles dispersed in a carbon matrix were produced by the controlled thermal decomposition of Fe(3+) ions in sucrose.
24519746	5	44	theme	Fenton-type	983:993	arg1	reaction					995:1002	a Fenton-type reaction	981:1002	a Fenton-type reaction	981:1002	The composites were tested for the oxidation of methylene blue with H2O2 by a Fenton-type reaction and also H2O2 decomposition, showing better performance for the material containing 8 % of iron heated at 400 and 600 °C.
24519746	3	45	theme	wt	484:485	arg1	contents					460:467	iron contents	455:467	iron contents of 1, 4, and 8 wt	455:485	The materials were prepared with iron contents of 1, 4, and 8 wt.
24519746	2	46	theme	sucrose	298:304	arg1	decomposition					306:318	the sucrose decomposition	294:318	the sucrose decomposition	294:318	During the sucrose decomposition, the Fe(3+) ions are reduced to form iron nanometric cores dispersed in a porous carbonaceous matrix.
24519746	4	47	theme	electron	700:707	arg1	microscopy					709:718	transmission electron microscopy	687:718	transmission electron microscopy	687:718	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	5	48	contain	containing	1077:1086	arg2	iron					1095:1098	iron	1095:1098	iron heated at 400 and 600 °C	1095:1123	The composites were tested for the oxidation of methylene blue with H2O2 by a Fenton-type reaction and also H2O2 decomposition, showing better performance for the material containing 8 % of iron heated at 400 and 600 °C.
24519746	5	48	contain	containing	1077:1086	arg1	material					1068:1075	the material	1064:1075	the material containing 8 % of iron heated at 400 and 600 °C	1064:1123	The composites were tested for the oxidation of methylene blue with H2O2 by a Fenton-type reaction and also H2O2 decomposition, showing better performance for the material containing 8 % of iron heated at 400 and 600 °C.
24519746	5	48	contain	containing	1077:1086	arg2	%					1090:1090	8 %	1088:1090	8 % of iron heated at 400 and 600 °C	1088:1123	The composites were tested for the oxidation of methylene blue with H2O2 by a Fenton-type reaction and also H2O2 decomposition, showing better performance for the material containing 8 % of iron heated at 400 and 600 °C.
24519746	0	49	with	contaminants	111:122	arg1	H₂O₂					129:132	H₂O₂	129:132	H₂O₂	129:132	Controlled formation of reactive Fe particles dispersed in a carbon matrix active for the oxidation of aqueous contaminants with H₂O₂.
24519746	4	50	theme	heated	493:498	arg1	area					667:670	heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area	493:670	heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area	493:670	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	0	51	theme	carbon	61:66	arg1	matrix					68:73	a carbon matrix	59:73	a carbon matrix active for the oxidation of aqueous contaminants with H₂O₂	59:132	Controlled formation of reactive Fe particles dispersed in a carbon matrix active for the oxidation of aqueous contaminants with H₂O₂.
24519746	6	52	theme	Fe3O4	1196:1200	arg1	nanoparticles					1202:1214	the Fe3O4 nanoparticles	1192:1214	the Fe3O4 nanoparticles active for the Fenton reaction	1192:1245	These results are discussed in terms of Fe(2+) surface species in the Fe3O4 nanoparticles active for the Fenton reaction.
24519746	2	53	theme	Fe	325:326	arg1	ions					332:335	the Fe(3+) ions	321:335	the Fe(3+) ions	321:335	During the sucrose decomposition, the Fe(3+) ions are reduced to form iron nanometric cores dispersed in a porous carbonaceous matrix.
24519746	4	54	theme	°C.	521:523	arg1	measurements					594:605	magnetization measurements	580:605	magnetization measurements	580:605	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	54	theme	°C.	521:523	arg1	analyses					645:652	termogravimetric analyses	628:652	termogravimetric analyses	628:652	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	54	theme	°C.	521:523	arg1	BET					655:657	BET	655:657	BET	655:657	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	54	theme	°C.	521:523	arg1	spectroscopy					566:577	Mössbauer spectroscopy	556:577	Mössbauer spectroscopy	556:577	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	54	theme	°C.	521:523	arg1	spectroscopy					614:625	Raman spectroscopy	608:625	Raman spectroscopy	608:625	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	54	theme	°C.	521:523	arg1	Analyses					525:532	400, 600, and 800 °C. Analyses	503:532	400, 600, and 800 °C. Analyses by X-ray diffraction	503:553	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	4	55	dep	showed	720:725	arg1	while					802:806	while	802:806	while	802:806	% and heated at 400, 600, and 800 °C. Analyses by X-ray diffraction, Mössbauer spectroscopy, magnetization measurements, Raman spectroscopy, termogravimetric analyses, BET surface area, scanning, and transmission electron microscopy showed that at 400 °C, the materials are composed essentially of Fe3O4 particles, while treatments at higher temperatures, i.e., 600 and 800 °C, produced phases such as Fe(0) and Fe3C.
24519746	3	56	theme	iron	455:458	arg1	contents					460:467	iron contents	455:467	iron contents of 1, 4, and 8 wt	455:485	The materials were prepared with iron contents of 1, 4, and 8 wt.
24519746	5	57	theme	iron	1095:1098	arg1	iron					1095:1098	iron	1095:1098	iron heated at 400 and 600 °C	1095:1123	The composites were tested for the oxidation of methylene blue with H2O2 by a Fenton-type reaction and also H2O2 decomposition, showing better performance for the material containing 8 % of iron heated at 400 and 600 °C.
24519746	5	57	theme	iron	1095:1098	arg1	%					1090:1090	8 %	1088:1090	8 % of iron heated at 400 and 600 °C	1088:1123	The composites were tested for the oxidation of methylene blue with H2O2 by a Fenton-type reaction and also H2O2 decomposition, showing better performance for the material containing 8 % of iron heated at 400 and 600 °C.
25489959	2	0	attach	present	367:373	arg2	groups					360:365	numerous phosphate groups	341:365	numerous phosphate groups present in the lipid A and core oligosaccharide regions	341:421	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	2	0	attach	present	367:373	arg1	regions					415:421	the lipid A and core oligosaccharide regions	378:421	the lipid A and core oligosaccharide regions	378:421	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	2	1	theme	numerous	341:348	arg1	groups					360:365	numerous phosphate groups	341:365	numerous phosphate groups present in the lipid A and core oligosaccharide regions	341:421	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	10	2	theme	asymmetric	1744:1753	arg1	models					1755:1760	the asymmetric models	1740:1760	the asymmetric models	1740:1760	This confirms the relevance of the asymmetric models for future studies of outer membrane stability and antibiotic penetration.
25489959	3	3	theme	divalent	521:528	arg1	cations					530:536	the divalent cations	517:536	the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM	517:623	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	3	3	theme	divalent	521:528	arg1	crucial					579:585	crucial	579:585	crucial	579:585	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	3	3	theme	divalent	521:528	arg1	Ca					550:551	Ca	550:551	Ca(2+)	550:555	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	3	3	theme	divalent	521:528	arg1	Mg					539:540	Mg	539:540	Mg(2+)	539:544	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	8	4	theme	inner	1528:1532	arg1	leaflets					1552:1559	the inner and outer bilayer leaflets	1524:1559	the inner and outer bilayer leaflets	1524:1559	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	5	5	theme	neutron	781:787	arg1	reflectivity					789:800	neutron reflectivity	781:800	neutron reflectivity	781:800	Here, we use X-ray and neutron reflectivity (XRR and NR, respectively) techniques to examine the role of calcium ions in the stability of a model GNB-OM.
25489959	10	6	theme	outer	1784:1788	arg1	stability					1799:1807	outer membrane stability	1784:1807	outer membrane stability	1784:1807	This confirms the relevance of the asymmetric models for future studies of outer membrane stability and antibiotic penetration.
25489959	8	7	theme	outer	1538:1542	arg1	leaflets					1552:1559	the inner and outer bilayer leaflets	1524:1559	the inner and outer bilayer leaflets	1524:1559	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	1	8	theme	lipid	174:178	arg1	composition					180:190	its lipid composition	170:190	its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS)	170:302	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	7	9	theme	RaLPS	1217:1221	arg1	bilayers					1223:1230	DPPC:RaLPS bilayers	1212:1230	DPPC:RaLPS bilayers	1212:1230	Using recently developed solid-supported models of the GNB-OM, we study the effect of calcium removal on the asymmetry of DPPC:RaLPS bilayers.
25489959	8	10	theme	charge	1258:1263	arg1	effect					1275:1280	the charge screening effect	1254:1280	the charge screening effect of divalent cations	1254:1300	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	7	11	theme	DPPC	1212:1215	arg1	bilayers					1223:1230	DPPC:RaLPS bilayers	1212:1230	DPPC:RaLPS bilayers	1212:1230	Using recently developed solid-supported models of the GNB-OM, we study the effect of calcium removal on the asymmetry of DPPC:RaLPS bilayers.
25489959	10	12	theme	antibiotic	1813:1822	arg1	penetration					1824:1834	antibiotic penetration	1813:1834	antibiotic penetration	1813:1834	This confirms the relevance of the asymmetric models for future studies of outer membrane stability and antibiotic penetration.
25489959	1	13	theme	phospholipid-rich	199:215	arg1	leaflet					223:229	a phospholipid-rich inner leaflet	197:229	a phospholipid-rich inner leaflet	197:229	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	6	14	theme	divalent	1057:1064	arg1	monolayers					1078:1087	divalent cation free monolayers	1057:1087	divalent cation free monolayers	1057:1087	Using XRR we show that Ca(2+) binds to the core region of the rough mutant LPS (RaLPS) films, producing more ordered structures in comparison to divalent cation free monolayers.
25489959	10	15	theme	stability	1799:1807	arg1	studies					1773:1779	future studies	1766:1779	future studies of outer membrane stability and antibiotic penetration	1766:1834	This confirms the relevance of the asymmetric models for future studies of outer membrane stability and antibiotic penetration.
25489959	1	16	from	composition	180:190	arg1	asymmetric					156:165	asymmetric	156:165	asymmetric	156:165	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	1	16	from	composition	180:190	arg1	membrane					135:142	The Gram-negative bacterial outer membrane	101:142	The Gram-negative bacterial outer membrane (GNB-OM)	101:151	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	1	17	theme	bacterial	119:127	arg1	GNB-OM					145:150	GNB-OM	145:150	GNB-OM	145:150	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	1	17	theme	bacterial	119:127	arg1	asymmetric					156:165	asymmetric	156:165	asymmetric	156:165	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	1	17	theme	bacterial	119:127	arg1	membrane					135:142	The Gram-negative bacterial outer membrane	101:142	The Gram-negative bacterial outer membrane (GNB-OM)	101:151	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	8	18	theme	divalent	1285:1292	arg1	cations					1294:1300	divalent cations	1285:1300	divalent cations	1285:1300	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	8	19	dep	mixing	1493:1498	arg1	%					1491:1491	%	1491:1491	%	1491:1491	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	3	20	dep	cations	530:536	arg1	cations					530:536	the divalent cations	517:536	the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM	517:623	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	3	20	dep	cations	530:536	arg1	2+					553:554	2+	553:554	2+	553:554	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	3	20	dep	cations	530:536	arg1	2+					542:543	2+	542:543	2+	542:543	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	3	20	dep	cations	530:536	arg1	Ca					550:551	Ca	550:551	Ca(2+)	550:555	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	3	20	dep	cations	530:536	arg1	Mg					539:540	Mg	539:540	Mg(2+)	539:544	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	7	21	theme	calcium	1176:1182	arg1	removal					1184:1190	calcium removal	1176:1190	calcium removal	1176:1190	Using recently developed solid-supported models of the GNB-OM, we study the effect of calcium removal on the asymmetry of DPPC:RaLPS bilayers.
25489959	1	22	from	asymmetric	156:165	arg1	composition					180:190	its lipid composition	170:190	its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS)	170:302	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	0	23	theme	bacterial	68:76	arg1	models					93:98	gram-negative bacterial outer membrane models	54:98	gram-negative bacterial outer membrane models	54:98	Effect of divalent cation removal on the structure of gram-negative bacterial outer membrane models.
25489959	0	24	from	Effect	0:5	arg1	structure					41:49	the structure	37:49	the structure of gram-negative bacterial outer membrane models	37:98	Effect of divalent cation removal on the structure of gram-negative bacterial outer membrane models.
25489959	6	25	dep	show	925:928	arg1	Using					912:916	Using	912:916	Using XRR	912:920	Using XRR we show that Ca(2+) binds to the core region of the rough mutant LPS (RaLPS) films, producing more ordered structures in comparison to divalent cation free monolayers.
25489959	0	26	theme	membrane	84:91	arg1	models					93:98	gram-negative bacterial outer membrane models	54:98	gram-negative bacterial outer membrane models	54:98	Effect of divalent cation removal on the structure of gram-negative bacterial outer membrane models.
25489959	5	27	theme	ions	871:874	arg1	role					855:858	the role	851:858	the role of calcium ions in the stability of a model GNB-OM	851:909	Here, we use X-ray and neutron reflectivity (XRR and NR, respectively) techniques to examine the role of calcium ions in the stability of a model GNB-OM.
25489959	9	28	theme	first	1591:1595	arg1	time					1597:1600	the first time	1587:1600	the first time	1587:1600	These results reveal for the first time the molecular details behind the well-known mechanism of outer membrane stabilization by divalent cations.
25489959	2	29	theme	A	388:388	arg1	regions					415:421	the lipid A and core oligosaccharide regions	378:421	the lipid A and core oligosaccharide regions	378:421	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	4	30	theme	cations	656:662	arg1	method					686:691	a well-established method	667:691	a well-established method to permeabilize Gram-negative bacteria such as Escherichia coli	667:755	Indeed, chelation of divalent cations is a well-established method to permeabilize Gram-negative bacteria such as Escherichia coli.
25489959	4	30	theme	cations	656:662	arg1	chelation					634:642	chelation	634:642	chelation of divalent cations	634:662	Indeed, chelation of divalent cations is a well-established method to permeabilize Gram-negative bacteria such as Escherichia coli.
25489959	8	31	theme	energy	1367:1372	arg1	barrier					1374:1380	the thermodynamically unfavorable energy barrier	1333:1380	the thermodynamically unfavorable energy barrier	1333:1380	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	0	32	theme	divalent	10:17	arg1	removal					26:32	divalent cation removal	10:32	divalent cation removal	10:32	Effect of divalent cation removal on the structure of gram-negative bacterial outer membrane models.
25489959	2	33	with	molecules	325:333	arg1	groups					360:365	numerous phosphate groups	341:365	numerous phosphate groups present in the lipid A and core oligosaccharide regions	341:421	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	2	34	theme	core	394:397	arg1	oligosaccharide					399:413	core oligosaccharide	394:413	core oligosaccharide	394:413	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	3	35	theme	due	445:447	arg1	forces					438:443	The repulsive forces	424:443	The repulsive forces due to accumulation of the negative charges	424:487	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	0	36	theme	removal	26:32	arg1	Effect					0:5	Effect	0:5	Effect of divalent cation removal on the structure of gram-negative bacterial outer membrane models.	0:99	Effect of divalent cation removal on the structure of gram-negative bacterial outer membrane models.
25489959	9	37	theme	well-known	1635:1644	arg1	mechanism					1646:1654	the well-known mechanism	1631:1654	the well-known mechanism of outer membrane stabilization by divalent cations	1631:1706	These results reveal for the first time the molecular details behind the well-known mechanism of outer membrane stabilization by divalent cations.
25489959	7	38	theme	GNB-OM	1145:1150	arg1	models					1131:1136	recently developed solid-supported models	1096:1136	recently developed solid-supported models of the GNB-OM	1096:1150	Using recently developed solid-supported models of the GNB-OM, we study the effect of calcium removal on the asymmetry of DPPC:RaLPS bilayers.
25489959	3	39	theme	charges	481:487	arg1	accumulation					452:463	accumulation	452:463	accumulation of the negative charges	452:487	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	6	40	theme	rough	974:978	arg1	films					999:1003	the rough mutant LPS (RaLPS) films	970:1003	the rough mutant LPS (RaLPS) films	970:1003	Using XRR we show that Ca(2+) binds to the core region of the rough mutant LPS (RaLPS) films, producing more ordered structures in comparison to divalent cation free monolayers.
25489959	9	41	theme	outer	1659:1663	arg1	stabilization					1674:1686	outer membrane stabilization	1659:1686	outer membrane stabilization by divalent cations	1659:1706	These results reveal for the first time the molecular details behind the well-known mechanism of outer membrane stabilization by divalent cations.
25489959	8	42	theme	hydrophobic	1402:1412	arg1	bilayer					1414:1420	the hydrophobic bilayer	1398:1420	the hydrophobic bilayer	1398:1420	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	1	43	theme	inner	217:221	arg1	leaflet					223:229	a phospholipid-rich inner leaflet	197:229	a phospholipid-rich inner leaflet	197:229	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	7	44	theme	solid-supported	1115:1129	arg1	models					1131:1136	recently developed solid-supported models	1096:1136	recently developed solid-supported models of the GNB-OM	1096:1150	Using recently developed solid-supported models of the GNB-OM, we study the effect of calcium removal on the asymmetry of DPPC:RaLPS bilayers.
25489959	9	45	theme	stabilization	1674:1686	arg1	mechanism					1646:1654	the well-known mechanism	1631:1654	the well-known mechanism of outer membrane stabilization by divalent cations	1631:1706	These results reveal for the first time the molecular details behind the well-known mechanism of outer membrane stabilization by divalent cations.
25489959	2	46	theme	present	367:373	arg1	groups					360:365	numerous phosphate groups	341:365	numerous phosphate groups present in the lipid A and core oligosaccharide regions	341:421	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	5	47	theme	model	898:902	arg1	GNB-OM					904:909	a model GNB-OM	896:909	a model GNB-OM	896:909	Here, we use X-ray and neutron reflectivity (XRR and NR, respectively) techniques to examine the role of calcium ions in the stability of a model GNB-OM.
25489959	2	48	from	regions	415:421	arg1	present					367:373	present	367:373	present	367:373	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	6	49	theme	core	955:958	arg1	region					960:965	the core region	951:965	the core region of the rough mutant LPS (RaLPS) films	951:1003	Using XRR we show that Ca(2+) binds to the core region of the rough mutant LPS (RaLPS) films, producing more ordered structures in comparison to divalent cation free monolayers.
25489959	9	50	theme	divalent	1691:1698	arg1	cations					1700:1706	divalent cations	1691:1706	divalent cations	1691:1706	These results reveal for the first time the molecular details behind the well-known mechanism of outer membrane stabilization by divalent cations.
25489959	2	51	theme	phosphate	350:358	arg1	groups					360:365	numerous phosphate groups	341:365	numerous phosphate groups present in the lipid A and core oligosaccharide regions	341:421	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	1	52	theme	outer	238:242	arg1	leaflet					244:250	an outer leaflet	235:250	an outer leaflet	235:250	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	8	53	theme	electrostatic	1448:1460	arg1	forces					1462:1467	the repulsive electrostatic forces	1434:1467	the repulsive electrostatic forces	1434:1467	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	3	54	theme	bacterial	612:620	arg1	OM					622:623	the bacterial OM	608:623	the bacterial OM	608:623	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	8	55	theme	LPS	1503:1505	arg1	mixing					1493:1498	about 20% mixing	1483:1498	about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets	1483:1559	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	10	56	theme	models	1755:1760	arg1	relevance					1727:1735	the relevance	1723:1735	the relevance of the asymmetric models for future studies of outer membrane stability and antibiotic penetration	1723:1834	This confirms the relevance of the asymmetric models for future studies of outer membrane stability and antibiotic penetration.
25489959	8	57	theme	DPPC	1511:1514	arg1	mixing					1493:1498	about 20% mixing	1483:1498	about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets	1483:1559	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	6	58	theme	films	999:1003	arg1	region					960:965	the core region	951:965	the core region of the rough mutant LPS (RaLPS) films	951:1003	Using XRR we show that Ca(2+) binds to the core region of the rough mutant LPS (RaLPS) films, producing more ordered structures in comparison to divalent cation free monolayers.
25489959	5	59	theme	reflectivity	789:800	arg1	techniques					829:838	X-ray and neutron reflectivity (XRR and NR, respectively) techniques	771:838	X-ray and neutron reflectivity (XRR and NR, respectively) techniques	771:838	Here, we use X-ray and neutron reflectivity (XRR and NR, respectively) techniques to examine the role of calcium ions in the stability of a model GNB-OM.
25489959	2	60	from	present	367:373	arg1	regions					415:421	the lipid A and core oligosaccharide regions	378:421	the lipid A and core oligosaccharide regions	378:421	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	6	61	theme	RaLPS	992:996	arg1	films					999:1003	the rough mutant LPS (RaLPS) films	970:1003	the rough mutant LPS (RaLPS) films	970:1003	Using XRR we show that Ca(2+) binds to the core region of the rough mutant LPS (RaLPS) films, producing more ordered structures in comparison to divalent cation free monolayers.
25489959	8	62	theme	screening	1265:1273	arg1	effect					1275:1280	the charge screening effect	1254:1280	the charge screening effect of divalent cations	1254:1300	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	7	63	theme	bilayers	1223:1230	arg1	asymmetry					1199:1207	the asymmetry	1195:1207	the asymmetry of DPPC:RaLPS bilayers	1195:1230	Using recently developed solid-supported models of the GNB-OM, we study the effect of calcium removal on the asymmetry of DPPC:RaLPS bilayers.
25489959	10	64	theme	future	1766:1771	arg1	studies					1773:1779	future studies	1766:1779	future studies of outer membrane stability and antibiotic penetration	1766:1834	This confirms the relevance of the asymmetric models for future studies of outer membrane stability and antibiotic penetration.
25489959	8	65	theme	bilayer	1544:1550	arg1	leaflets					1552:1559	the inner and outer bilayer leaflets	1524:1559	the inner and outer bilayer leaflets	1524:1559	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	6	66	theme	cation	1066:1071	arg1	monolayers					1078:1087	divalent cation free monolayers	1057:1087	divalent cation free monolayers	1057:1087	Using XRR we show that Ca(2+) binds to the core region of the rough mutant LPS (RaLPS) films, producing more ordered structures in comparison to divalent cation free monolayers.
25489959	1	67	theme	Gram-negative	105:117	arg1	GNB-OM					145:150	GNB-OM	145:150	GNB-OM	145:150	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	1	67	theme	Gram-negative	105:117	arg1	asymmetric					156:165	asymmetric	156:165	asymmetric	156:165	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	1	67	theme	Gram-negative	105:117	arg1	membrane					135:142	The Gram-negative bacterial outer membrane	101:142	The Gram-negative bacterial outer membrane (GNB-OM)	101:151	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	8	68	theme	cations	1294:1300	arg1	effect					1275:1280	the charge screening effect	1254:1280	the charge screening effect of divalent cations	1254:1300	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	10	69	theme	membrane	1790:1797	arg1	stability					1799:1807	outer membrane stability	1784:1807	outer membrane stability	1784:1807	This confirms the relevance of the asymmetric models for future studies of outer membrane stability and antibiotic penetration.
25489959	1	70	theme	outer	129:133	arg1	GNB-OM					145:150	GNB-OM	145:150	GNB-OM	145:150	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	1	70	theme	outer	129:133	arg1	asymmetric					156:165	asymmetric	156:165	asymmetric	156:165	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	1	70	theme	outer	129:133	arg1	membrane					135:142	The Gram-negative bacterial outer membrane	101:142	The Gram-negative bacterial outer membrane (GNB-OM)	101:151	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	8	71	dep	%	1491:1491	arg1	20					1489:1490	20	1489:1490	20	1489:1490	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	0	72	theme	gram-negative	54:66	arg1	models					93:98	gram-negative bacterial outer membrane models	54:98	gram-negative bacterial outer membrane models	54:98	Effect of divalent cation removal on the structure of gram-negative bacterial outer membrane models.
25489959	9	73	theme	membrane	1665:1672	arg1	stabilization					1674:1686	outer membrane stabilization	1659:1686	outer membrane stabilization by divalent cations	1659:1706	These results reveal for the first time the molecular details behind the well-known mechanism of outer membrane stabilization by divalent cations.
25489959	7	74	theme	removal	1184:1190	arg1	effect					1166:1171	the effect	1162:1171	the effect of calcium removal on the asymmetry of DPPC:RaLPS bilayers	1162:1230	Using recently developed solid-supported models of the GNB-OM, we study the effect of calcium removal on the asymmetry of DPPC:RaLPS bilayers.
25489959	10	75	theme	penetration	1824:1834	arg1	studies					1773:1779	future studies	1766:1779	future studies of outer membrane stability and antibiotic penetration	1766:1834	This confirms the relevance of the asymmetric models for future studies of outer membrane stability and antibiotic penetration.
25489959	0	76	theme	outer	78:82	arg1	models					93:98	gram-negative bacterial outer membrane models	54:98	gram-negative bacterial outer membrane models	54:98	Effect of divalent cation removal on the structure of gram-negative bacterial outer membrane models.
25489959	0	77	theme	models	93:98	arg1	structure					41:49	the structure	37:49	the structure of gram-negative bacterial outer membrane models	37:98	Effect of divalent cation removal on the structure of gram-negative bacterial outer membrane models.
25489959	4	78	theme	Gram-negative	709:721	arg1	coli					752:755	Escherichia coli	740:755	Escherichia coli	740:755	Indeed, chelation of divalent cations is a well-established method to permeabilize Gram-negative bacteria such as Escherichia coli.
25489959	4	78	theme	Gram-negative	709:721	arg1	bacteria					723:730	Gram-negative bacteria	709:730	Gram-negative bacteria such as Escherichia coli	709:755	Indeed, chelation of divalent cations is a well-established method to permeabilize Gram-negative bacteria such as Escherichia coli.
25489959	5	79	theme	calcium	863:869	arg1	ions					871:874	calcium ions	863:874	calcium ions	863:874	Here, we use X-ray and neutron reflectivity (XRR and NR, respectively) techniques to examine the role of calcium ions in the stability of a model GNB-OM.
25489959	6	80	theme	LPS	987:989	arg1	films					999:1003	the rough mutant LPS (RaLPS) films	970:1003	the rough mutant LPS (RaLPS) films	970:1003	Using XRR we show that Ca(2+) binds to the core region of the rough mutant LPS (RaLPS) films, producing more ordered structures in comparison to divalent cation free monolayers.
25489959	4	81	theme	well-established	669:684	arg1	method					686:691	a well-established method	667:691	a well-established method to permeabilize Gram-negative bacteria such as Escherichia coli	667:755	Indeed, chelation of divalent cations is a well-established method to permeabilize Gram-negative bacteria such as Escherichia coli.
25489959	4	81	theme	well-established	669:684	arg1	chelation					634:642	chelation	634:642	chelation of divalent cations	634:662	Indeed, chelation of divalent cations is a well-established method to permeabilize Gram-negative bacteria such as Escherichia coli.
25489959	2	82	theme	polyanionic	313:323	arg1	LPS					305:307	LPS	305:307	LPS	305:307	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	2	82	theme	polyanionic	313:323	arg1	molecules					325:333	polyanionic molecules	313:333	polyanionic molecules	313:333	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	8	83	theme	unfavorable	1355:1365	arg1	barrier					1374:1380	the thermodynamically unfavorable energy barrier	1333:1380	the thermodynamically unfavorable energy barrier	1333:1380	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	2	84	theme	lipid	382:386	arg1	A					388:388	lipid A	382:388	lipid A	382:388	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	9	85	theme	molecular	1606:1614	arg1	details					1616:1622	the molecular details	1602:1622	the molecular details behind the well-known mechanism of outer membrane stabilization by divalent cations	1602:1706	These results reveal for the first time the molecular details behind the well-known mechanism of outer membrane stabilization by divalent cations.
25489959	4	86	theme	divalent	647:654	arg1	cations					656:662	divalent cations	647:662	divalent cations	647:662	Indeed, chelation of divalent cations is a well-established method to permeabilize Gram-negative bacteria such as Escherichia coli.
25489959	0	87	theme	cation	19:24	arg1	removal					26:32	divalent cation removal	10:32	divalent cation removal	10:32	Effect of divalent cation removal on the structure of gram-negative bacterial outer membrane models.
25489959	6	88	theme	free	1073:1076	arg1	monolayers					1078:1087	divalent cation free monolayers	1057:1087	divalent cation free monolayers	1057:1087	Using XRR we show that Ca(2+) binds to the core region of the rough mutant LPS (RaLPS) films, producing more ordered structures in comparison to divalent cation free monolayers.
25489959	2	89	theme	oligosaccharide	399:413	arg1	regions					415:421	the lipid A and core oligosaccharide regions	378:421	the lipid A and core oligosaccharide regions	378:421	LPS are polyanionic molecules, with numerous phosphate groups present in the lipid A and core oligosaccharide regions.
25489959	1	90	with	composition	180:190	arg1	leaflet					244:250	an outer leaflet	235:250	an outer leaflet	235:250	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	1	90	with	composition	180:190	arg1	leaflet					223:229	a phospholipid-rich inner leaflet	197:229	a phospholipid-rich inner leaflet	197:229	The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS).
25489959	5	91	theme	GNB-OM	904:909	arg1	stability					883:891	the stability	879:891	the stability of a model GNB-OM	879:909	Here, we use X-ray and neutron reflectivity (XRR and NR, respectively) techniques to examine the role of calcium ions in the stability of a model GNB-OM.
25489959	6	92	theme	ordered	1021:1027	arg1	structures					1029:1038	more ordered structures	1016:1038	more ordered structures	1016:1038	Using XRR we show that Ca(2+) binds to the core region of the rough mutant LPS (RaLPS) films, producing more ordered structures in comparison to divalent cation free monolayers.
25489959	3	93	theme	repulsive	428:436	arg1	forces					438:443	The repulsive forces	424:443	The repulsive forces due to accumulation of the negative charges	424:487	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	6	94	theme	mutant	980:985	arg1	films					999:1003	the rough mutant LPS (RaLPS) films	970:1003	the rough mutant LPS (RaLPS) films	970:1003	Using XRR we show that Ca(2+) binds to the core region of the rough mutant LPS (RaLPS) films, producing more ordered structures in comparison to divalent cation free monolayers.
25489959	5	95	from	role	855:858	arg1	stability					883:891	the stability	879:891	the stability of a model GNB-OM	879:909	Here, we use X-ray and neutron reflectivity (XRR and NR, respectively) techniques to examine the role of calcium ions in the stability of a model GNB-OM.
25489959	7	96	theme	developed	1105:1113	arg1	models					1131:1136	recently developed solid-supported models	1096:1136	recently developed solid-supported models of the GNB-OM	1096:1150	Using recently developed solid-supported models of the GNB-OM, we study the effect of calcium removal on the asymmetry of DPPC:RaLPS bilayers.
25489959	7	97	from	effect	1166:1171	arg1	asymmetry					1199:1207	the asymmetry	1195:1207	the asymmetry of DPPC:RaLPS bilayers	1195:1230	Using recently developed solid-supported models of the GNB-OM, we study the effect of calcium removal on the asymmetry of DPPC:RaLPS bilayers.
25489959	5	98	dep	X-ray	771:775	arg1	NR					811:812	NR	811:812	NR	811:812	Here, we use X-ray and neutron reflectivity (XRR and NR, respectively) techniques to examine the role of calcium ions in the stability of a model GNB-OM.
25489959	5	98	dep	X-ray	771:775	arg1	XRR					803:805	XRR	803:805	XRR	803:805	Here, we use X-ray and neutron reflectivity (XRR and NR, respectively) techniques to examine the role of calcium ions in the stability of a model GNB-OM.
25489959	3	99	theme	negative	472:479	arg1	charges					481:487	the negative charges	468:487	the negative charges	468:487	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
25489959	5	100	theme	X-ray	771:775	arg1	techniques					829:838	X-ray and neutron reflectivity (XRR and NR, respectively) techniques	771:838	X-ray and neutron reflectivity (XRR and NR, respectively) techniques	771:838	Here, we use X-ray and neutron reflectivity (XRR and NR, respectively) techniques to examine the role of calcium ions in the stability of a model GNB-OM.
25489959	8	101	theme	repulsive	1438:1446	arg1	forces					1462:1467	the repulsive electrostatic forces	1434:1467	the repulsive electrostatic forces	1434:1467	We show that without the charge screening effect of divalent cations, the LPS is forced to overcome the thermodynamically unfavorable energy barrier and flip across the hydrophobic bilayer to minimize the repulsive electrostatic forces, resulting in about 20% mixing of LPS and DPPC between the inner and outer bilayer leaflets.
25489959	3	102	theme	OM	622:623	arg1	integrity					595:603	the integrity	591:603	the integrity of the bacterial OM	591:623	The repulsive forces due to accumulation of the negative charges are screened and bridged by the divalent cations (Mg(2+) and Ca(2+)) that are known to be crucial for the integrity of the bacterial OM.
28776757	5	0	theme	IL-4	1161:1164	arg1	cytokines					1176:1184	the IFN-γ, IL-4, and IL-6 cytokines	1150:1184	the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls	1150:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	1	1	theme	response	157:164	arg1	polarization					134:145	Th1 polarization	130:145	Th1 polarization of immune response	130:164	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	3	2	theme	months	788:793	arg1	period					776:781	a period	774:781	a period of 6 months	774:793	METHODS The immune responses of the 45 patients with RAS, submitted to symbiotic or placebo for 120 days, in relation to 30 RAS-free controls, were evaluated over a period of 6 months.
28776757	7	3	theme	Th1	1711:1713	arg1	direction					1698:1706	the direction	1694:1706	the direction of Th1 and improved pain symptomatology	1694:1746	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	1	4	dep	BACKGROUND	92:101	arg1	are					109:111	are	109:111	are	109:111	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	6	5	from	increase	1444:1451	arg1	pain					1432:1435	pain	1432:1435	pain	1432:1435	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	5	from	increase	1444:1451	arg1	levels					1462:1467	IFN-γ levels	1456:1467	IFN-γ levels	1456:1467	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	5	6	with	comparison	1209:1218	arg1	controls					1229:1236	the controls	1225:1236	the controls	1225:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	7	7	theme	immune	1676:1681	arg1	profile					1683:1689	the Th2 serological immune profile	1656:1689	the Th2 serological immune profile	1656:1689	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	6	8	dep	clusters	1326:1333	arg1	clusters					1326:1333	2 distinct clusters	1315:1333	2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype	1315:1375	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	8	dep	clusters	1326:1333	arg1	subtype					1369:1375	a Mixed (Th1/Th2) subtype	1351:1375	a Mixed (Th1/Th2) subtype	1351:1375	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	8	dep	clusters	1326:1333	arg1	Th2					1343:1345	a pure Th2	1336:1345	a pure Th2	1336:1345	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	9	theme	IFN-γ	1456:1460	arg1	levels					1462:1467	IFN-γ levels	1456:1467	IFN-γ levels	1456:1467	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	10	from	improvement	1417:1427	arg1	pain					1432:1435	pain	1432:1435	pain	1432:1435	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	10	from	improvement	1417:1427	arg1	levels					1462:1467	IFN-γ levels	1456:1467	IFN-γ levels	1456:1467	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	7	11	from	alteration	1642:1651	arg1	profile					1683:1689	the Th2 serological immune profile	1656:1689	the Th2 serological immune profile	1656:1689	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	7	12	theme	Th2	1660:1662	arg1	profile					1683:1689	the Th2 serological immune profile	1656:1689	the Th2 serological immune profile	1656:1689	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	4	13	theme	clinical	1034:1041	arg1	parameters					1043:1052	clinical parameters	1034:1052	clinical parameters	1034:1052	Peripheral blood was collected from all patients at 0 (T0), 120 (T4), and 180 days (T6) after the start of treatment and Th1 (IL12-p70, IFN-γ), Th2 (IL-4), Treg (IL-10), Th17 (IL-17A), inflammatory (TNF-α, IL-6)-associated cytokines, and clinical parameters were quantified.
28776757	1	14	dep	indications	113:123	arg1	stimulate					285:293	stimulate	285:293	can stimulate immune regulatory activity	281:320	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	1	14	dep	indications	113:123	arg1	plays					166:170	plays	166:170	plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS)	166:247	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	5	15	theme	serological	1128:1138	arg1	levels					1140:1145	the serological levels	1124:1145	the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls	1124:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	2	16	theme	serological	473:483	arg1	response					485:492	clinical and serological response	460:492	clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium	460:608	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	6	17	theme	pure	1338:1341	arg1	clusters					1326:1333	2 distinct clusters	1315:1333	2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype	1315:1375	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	17	theme	pure	1338:1341	arg1	Th2					1343:1345	a pure Th2	1336:1345	a pure Th2	1336:1345	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	2	18	theme	patients	438:445	arg1	profile					423:429	the initial immune profile	404:429	the initial immune profile of RAS patients	404:445	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	6	19	theme	Th1/Th2	1360:1366	arg1	clusters					1326:1333	2 distinct clusters	1315:1333	2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype	1315:1375	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	19	theme	Th1/Th2	1360:1366	arg1	subtype					1369:1375	a Mixed (Th1/Th2) subtype	1351:1375	a Mixed (Th1/Th2) subtype	1351:1375	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	2	20	theme	initial	408:414	arg1	profile					423:429	the initial immune profile	404:429	the initial immune profile of RAS patients	404:445	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	1	21	theme	important	175:183	arg1	role					185:188	an important role	172:188	an important role	172:188	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	2	22	theme	immune	416:421	arg1	profile					423:429	the initial immune profile	404:429	the initial immune profile of RAS patients	404:445	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	1	23	theme	disease	353:359	arg1	course					339:344	the course	335:344	the course of the disease	335:359	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	7	24	dep	Th1	1711:1713	arg1	symptomatology					1733:1746	symptomatology	1733:1746	symptomatology	1733:1746	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	5	25	dep	RESULTS	1071:1077	arg1	found					1115:1119	found	1115:1119	were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls	1110:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	6	26	theme	group	1292:1296	arg1	profile					1273:1279	the cytokine profile	1260:1279	the cytokine profile of the RAS group	1260:1296	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	0	27	theme	Immune	0:5	arg1	response					7:14	Immune response	0:14	Immune response of patients with recurrent aphthous stomatitis	0:61	Immune response of patients with recurrent aphthous stomatitis challenged with a symbiotic.
28776757	5	28	theme	RAS	1193:1195	arg1	patients					1197:1204	the RAS patients	1189:1204	the RAS patients in comparison with the controls	1189:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	4	29	theme	treatment	903:911	arg1	start					894:898	the start	890:898	the start of treatment	890:911	Peripheral blood was collected from all patients at 0 (T0), 120 (T4), and 180 days (T6) after the start of treatment and Th1 (IL12-p70, IFN-γ), Th2 (IL-4), Treg (IL-10), Th17 (IL-17A), inflammatory (TNF-α, IL-6)-associated cytokines, and clinical parameters were quantified.
28776757	2	30	contain	containing	541:550	arg2	Lactobacillus					575:587	Lactobacillus	575:587	Lactobacillus	575:587	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	2	30	contain	containing	541:550	arg2	fructooligosaccharide					552:572	fructooligosaccharide	552:572	fructooligosaccharide	552:572	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	2	30	contain	containing	541:550	arg1	treatment					531:539	symbiotic treatment	521:539	symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium	521:608	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	2	30	contain	containing	541:550	arg2	Bifidobacterium					594:608	Bifidobacterium	594:608	Bifidobacterium	594:608	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	0	31	theme	patients	19:26	arg1	response					7:14	Immune response	0:14	Immune response of patients with recurrent aphthous stomatitis	0:61	Immune response of patients with recurrent aphthous stomatitis challenged with a symbiotic.
28776757	5	32	theme	patients	1197:1204	arg1	cytokines					1176:1184	the IFN-γ, IL-4, and IL-6 cytokines	1150:1184	the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls	1150:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	4	33	theme	-associated	1007:1017	arg1	cytokines					1019:1027	inflammatory (TNF-α, IL-6)-associated cytokines	981:1027	inflammatory (TNF-α, IL-6)-associated cytokines	981:1027	Peripheral blood was collected from all patients at 0 (T0), 120 (T4), and 180 days (T6) after the start of treatment and Th1 (IL12-p70, IFN-γ), Th2 (IL-4), Treg (IL-10), Th17 (IL-17A), inflammatory (TNF-α, IL-6)-associated cytokines, and clinical parameters were quantified.
28776757	4	33	theme	-associated	1007:1017	arg1	TNF-α					995:999	TNF-α	995:999	TNF-α	995:999	Peripheral blood was collected from all patients at 0 (T0), 120 (T4), and 180 days (T6) after the start of treatment and Th1 (IL12-p70, IFN-γ), Th2 (IL-4), Treg (IL-10), Th17 (IL-17A), inflammatory (TNF-α, IL-6)-associated cytokines, and clinical parameters were quantified.
28776757	4	34	dep	TNF-α	995:999	arg1	IL-6					1002:1005	IL-6	1002:1005	IL-6	1002:1005	Peripheral blood was collected from all patients at 0 (T0), 120 (T4), and 180 days (T6) after the start of treatment and Th1 (IL12-p70, IFN-γ), Th2 (IL-4), Treg (IL-10), Th17 (IL-17A), inflammatory (TNF-α, IL-6)-associated cytokines, and clinical parameters were quantified.
28776757	3	35	dep	METHODS	611:617	arg1	evaluated					759:767	evaluated	759:767	were evaluated over a period of 6 months	754:793	METHODS The immune responses of the 45 patients with RAS, submitted to symbiotic or placebo for 120 days, in relation to 30 RAS-free controls, were evaluated over a period of 6 months.
28776757	7	36	theme	pain	1728:1731	arg1	direction					1698:1706	the direction	1694:1706	the direction of Th1 and improved pain symptomatology	1694:1746	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	7	37	theme	symbiotic	1529:1537	arg1	treatment					1539:1547	symbiotic treatment	1529:1547	symbiotic treatment based on a fructooligosaccharide	1529:1580	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	2	38	theme	symbiotic	521:529	arg1	treatment					531:539	symbiotic treatment	521:539	symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium	521:608	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	5	39	from	comparison	1209:1218	arg1	cytokines					1176:1184	the IFN-γ, IL-4, and IL-6 cytokines	1150:1184	the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls	1150:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	5	40	from	cytokines	1176:1184	arg1	comparison					1209:1218	comparison	1209:1218	comparison with the controls	1209:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	0	41	theme	recurrent	33:41	arg1	stomatitis					52:61	recurrent aphthous stomatitis	33:61	recurrent aphthous stomatitis	33:61	Immune response of patients with recurrent aphthous stomatitis challenged with a symbiotic.
28776757	1	42	theme	probiotics	270:279	arg1	use					263:265	the use	259:265	the use of probiotics	259:279	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	3	43	theme	patients	650:657	arg1	responses					630:638	The immune responses	619:638	The immune responses of the 45 patients with RAS, submitted to symbiotic or placebo for 120 days	619:714	METHODS The immune responses of the 45 patients with RAS, submitted to symbiotic or placebo for 120 days, in relation to 30 RAS-free controls, were evaluated over a period of 6 months.
28776757	3	44	with	patients	650:657	arg1	RAS					664:666	RAS	664:666	RAS	664:666	METHODS The immune responses of the 45 patients with RAS, submitted to symbiotic or placebo for 120 days, in relation to 30 RAS-free controls, were evaluated over a period of 6 months.
28776757	2	45	theme	study	378:382	arg1	aim					366:368	The aim	362:368	The aim of this study	362:382	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	2	46	theme	RAS	434:436	arg1	patients					438:445	RAS patients	434:445	RAS patients	434:445	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	3	47	theme	RAS-free	735:742	arg1	controls					744:751	30 RAS-free controls	732:751	30 RAS-free controls	732:751	METHODS The immune responses of the 45 patients with RAS, submitted to symbiotic or placebo for 120 days, in relation to 30 RAS-free controls, were evaluated over a period of 6 months.
28776757	7	48	theme	improved	1719:1726	arg1	pain					1728:1731	improved pain	1719:1731	improved pain	1719:1731	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	0	49	with	patients	19:26	arg1	stomatitis					52:61	recurrent aphthous stomatitis	33:61	recurrent aphthous stomatitis	33:61	Immune response of patients with recurrent aphthous stomatitis challenged with a symbiotic.
28776757	1	50	theme	recurrent	213:221	arg1	RAS					244:246	RAS	244:246	RAS	244:246	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	1	50	theme	recurrent	213:221	arg1	stomatitis					232:241	recurrent aphthous stomatitis	213:241	recurrent aphthous stomatitis (RAS)	213:247	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	6	51	theme	Th2	1495:1497	arg1	response					1499:1506	Th2 response	1495:1506	Th2 response	1495:1506	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	4	52	theme	inflammatory	981:992	arg1	cytokines					1019:1027	inflammatory (TNF-α, IL-6)-associated cytokines	981:1027	inflammatory (TNF-α, IL-6)-associated cytokines	981:1027	Peripheral blood was collected from all patients at 0 (T0), 120 (T4), and 180 days (T6) after the start of treatment and Th1 (IL12-p70, IFN-γ), Th2 (IL-4), Treg (IL-10), Th17 (IL-17A), inflammatory (TNF-α, IL-6)-associated cytokines, and clinical parameters were quantified.
28776757	4	52	theme	inflammatory	981:992	arg1	TNF-α					995:999	TNF-α	995:999	TNF-α	995:999	Peripheral blood was collected from all patients at 0 (T0), 120 (T4), and 180 days (T6) after the start of treatment and Th1 (IL12-p70, IFN-γ), Th2 (IL-4), Treg (IL-10), Th17 (IL-17A), inflammatory (TNF-α, IL-6)-associated cytokines, and clinical parameters were quantified.
28776757	0	53	theme	aphthous	43:50	arg1	stomatitis					52:61	recurrent aphthous stomatitis	33:61	recurrent aphthous stomatitis	33:61	Immune response of patients with recurrent aphthous stomatitis challenged with a symbiotic.
28776757	1	54	theme	immune	295:300	arg1	activity					313:320	immune regulatory activity	295:320	immune regulatory activity	295:320	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	6	55	theme	RAS	1288:1290	arg1	group					1292:1296	the RAS group	1284:1296	the RAS group	1284:1296	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	4	56	theme	Peripheral	796:805	arg1	blood					807:811	Peripheral blood	796:811	Peripheral blood	796:811	Peripheral blood was collected from all patients at 0 (T0), 120 (T4), and 180 days (T6) after the start of treatment and Th1 (IL12-p70, IFN-γ), Th2 (IL-4), Treg (IL-10), Th17 (IL-17A), inflammatory (TNF-α, IL-6)-associated cytokines, and clinical parameters were quantified.
28776757	5	57	theme	IL-6	1171:1174	arg1	cytokines					1176:1184	the IFN-γ, IL-4, and IL-6 cytokines	1150:1184	the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls	1150:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	5	58	from	patients	1197:1204	arg1	comparison					1209:1218	comparison	1209:1218	comparison with the controls	1209:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	6	59	theme	cytokine	1264:1271	arg1	profile					1273:1279	the cytokine profile	1260:1279	the cytokine profile of the RAS group	1260:1296	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	1	60	theme	regulatory	302:311	arg1	activity					313:320	immune regulatory activity	295:320	immune regulatory activity	295:320	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	3	61	theme	immune	623:628	arg1	responses					630:638	The immune responses	619:638	The immune responses of the 45 patients with RAS, submitted to symbiotic or placebo for 120 days	619:714	METHODS The immune responses of the 45 patients with RAS, submitted to symbiotic or placebo for 120 days, in relation to 30 RAS-free controls, were evaluated over a period of 6 months.
28776757	5	62	theme	cytokines	1176:1184	arg1	levels					1140:1145	the serological levels	1124:1145	the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls	1124:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	1	63	theme	Th1	130:132	arg1	polarization					134:145	Th1 polarization	130:145	Th1 polarization of immune response	130:164	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	2	64	with	challenge	506:514	arg1	treatment					531:539	symbiotic treatment	521:539	symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium	521:608	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	1	65	theme	aphthous	223:230	arg1	RAS					244:246	RAS	244:246	RAS	244:246	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	1	65	theme	aphthous	223:230	arg1	stomatitis					232:241	recurrent aphthous stomatitis	213:241	recurrent aphthous stomatitis (RAS)	213:247	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	5	66	located	found	1115:1119	arg1	T0					1082:1083	T0	1082:1083	T0	1082:1083	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	5	66	located	found	1115:1119	arg1	levels					1140:1145	the serological levels	1124:1145	the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls	1124:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	5	66	located	found	1115:1119	arg2	differences					1098:1108	significant differences	1086:1108	significant differences	1086:1108	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	6	67	from	reduction	1482:1490	arg1	response					1499:1506	Th2 response	1495:1506	Th2 response	1495:1506	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	7	68	from	CONCLUSIONS	1509:1519	arg1	treatment					1539:1547	symbiotic treatment	1529:1547	symbiotic treatment based on a fructooligosaccharide	1529:1580	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	7	68	from	CONCLUSIONS	1509:1519	arg1	Lactobacillus					1583:1595	Lactobacillus	1583:1595	Lactobacillus	1583:1595	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	7	68	from	CONCLUSIONS	1509:1519	arg1	composition					1618:1628	Bifidobacterium composition	1602:1628	Bifidobacterium composition	1602:1628	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	7	68	from	CONCLUSIONS	1509:1519	arg1	RAS					1524:1526	RAS	1524:1526	RAS	1524:1526	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	1	69	theme	stomatitis	232:241	arg1	pathogenesis					197:208	the pathogenesis	193:208	the pathogenesis of recurrent aphthous stomatitis (RAS)	193:247	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
28776757	7	70	theme	Bifidobacterium	1602:1616	arg1	composition					1618:1628	Bifidobacterium composition	1602:1628	Bifidobacterium composition	1602:1628	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	6	71	theme	symbiotic	1386:1394	arg1	treatment					1396:1404	symbiotic treatment	1386:1404	symbiotic treatment	1386:1404	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	5	72	theme	IFN-γ	1154:1158	arg1	cytokines					1176:1184	the IFN-γ, IL-4, and IL-6 cytokines	1150:1184	the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls	1150:1236	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	5	73	theme	significant	1086:1096	arg1	differences					1098:1108	significant differences	1086:1108	significant differences	1086:1108	RESULTS At T0, significant differences were found in the serological levels of the IFN-γ, IL-4, and IL-6 cytokines of the RAS patients in comparison with the controls.
28776757	7	74	theme	serological	1664:1674	arg1	profile					1683:1689	the Th2 serological immune profile	1656:1689	the Th2 serological immune profile	1656:1689	CONCLUSIONS In RAS, symbiotic treatment based on a fructooligosaccharide, Lactobacillus, and Bifidobacterium composition produced an alteration in the Th2 serological immune profile in the direction of Th1 and improved pain symptomatology.
28776757	2	75	theme	clinical	460:467	arg1	response					485:492	clinical and serological response	460:492	clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium	460:608	The aim of this study was to characterize the initial immune profile of RAS patients and evaluate clinical and serological response following a challenge with symbiotic treatment containing fructooligosaccharide, Lactobacillus, and Bifidobacterium.
28776757	6	76	theme	Mixed	1353:1357	arg1	clusters					1326:1333	2 distinct clusters	1315:1333	2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype	1315:1375	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	76	theme	Mixed	1353:1357	arg1	subtype					1369:1375	a Mixed (Th1/Th2) subtype	1351:1375	a Mixed (Th1/Th2) subtype	1351:1375	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	77	theme	distinct	1317:1324	arg1	clusters					1326:1333	2 distinct clusters	1315:1333	2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype	1315:1375	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	77	theme	distinct	1317:1324	arg1	subtype					1369:1375	a Mixed (Th1/Th2) subtype	1351:1375	a Mixed (Th1/Th2) subtype	1351:1375	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	6	77	theme	distinct	1317:1324	arg1	Th2					1343:1345	a pure Th2	1336:1345	a pure Th2	1336:1345	It was observed that the cytokine profile of the RAS group was comprised of 2 distinct clusters: a pure Th2 and a Mixed (Th1/Th2) subtype and that symbiotic treatment induced an improvement in pain and an increase in IFN-γ levels, producing a reduction in Th2 response.
28776757	1	78	theme	immune	150:155	arg1	response					157:164	immune response	150:164	immune response	150:164	BACKGROUND There are indications that Th1 polarization of immune response plays an important role in the pathogenesis of recurrent aphthous stomatitis (RAS), and that the use of probiotics can stimulate immune regulatory activity, influencing the course of the disease.
24711342	0	0	theme	potential	90:98	arg1	application					100:110	their potential application	84:110	their potential application in nerve regeneration	84:132	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	0	1	from	synthesis	8:16	arg1	regeneration					121:132	nerve regeneration	115:132	nerve regeneration	115:132	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	6	2	theme	Electrical	1035:1044	arg1	stimulation					1046:1056	Electrical stimulation	1035:1056	Electrical stimulation	1035:1056	Electrical stimulation demonstrated that PC12 cells attached and extended longer neurites when cultured on NCG/PPy composite gels with DBSA dopant.
24711342	1	3	theme	analogous	180:188	arg1	environments					190:201	analogous environments	180:201	analogous environments for extracellular matrix	180:226	Nanostructured conductive polymers can offer analogous environments for extracellular matrix and induce cellular responses by electric stimulation, however, such materials often lack mechanical strength and tend to collapse under small stresses.
24711342	3	4	theme	high	795:798	arg1	strength					811:818	high mechanical strength	795:818	high mechanical strength	795:818	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	3	5	theme	nitrogen	734:741	arg1	0.41-0.53 g cm					714:727	0.41-0.53 g cm(-3)	714:731	0.41-0.53 g cm(-3)	714:731	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	3	5	theme	nitrogen	734:741	arg1	areas					762:766	nitrogen adsorption surface areas	734:766	nitrogen adsorption surface areas of 264-303 m(2) g(-1)	734:788	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	4	6	theme	electrical	866:875	arg1	conductivity					877:888	an electrical conductivity	863:888	an electrical conductivity of up to 0.08 S cm(-1)	863:911	The NCG/PPy composite hydrogels exhibited an electrical conductivity of up to 0.08 S cm(-1).
24711342	1	7	theme	such	292:295	arg1	materials					297:305	such materials	292:305	such materials	292:305	Nanostructured conductive polymers can offer analogous environments for extracellular matrix and induce cellular responses by electric stimulation, however, such materials often lack mechanical strength and tend to collapse under small stresses.
24711342	7	8	theme	promising	1203:1211	arg1	materials					1189:1197	These materials	1183:1197	These materials	1183:1197	These materials are promising candidates for applications in nerve regeneration, carbon capture, catalyst supports, and many others.
24711342	7	8	theme	promising	1203:1211	arg1	candidates					1213:1222	promising candidates	1203:1222	promising candidates for applications in nerve regeneration, carbon capture, catalyst supports, and many others	1203:1313	These materials are promising candidates for applications in nerve regeneration, carbon capture, catalyst supports, and many others.
24711342	6	9	theme	NCG/PPy	1142:1148	arg1	gels					1160:1163	NCG/PPy composite gels	1142:1163	NCG/PPy composite gels with DBSA dopant	1142:1180	Electrical stimulation demonstrated that PC12 cells attached and extended longer neurites when cultured on NCG/PPy composite gels with DBSA dopant.
24711342	7	10	dep	catalyst	1280:1287	arg1	supports					1289:1296	supports	1289:1296	supports	1289:1296	These materials are promising candidates for applications in nerve regeneration, carbon capture, catalyst supports, and many others.
24711342	5	11	theme	PPy	964:966	arg1	incorporation					947:959	the incorporation	943:959	the incorporation of PPy into an NCG	943:978	In vitro studies showed that the incorporation of PPy into an NCG enhances the adhesion and proliferation of PC12 cells.
24711342	2	12	theme	pyrrole	555:561	arg1	monomers					563:570	pyrrole monomers	555:570	pyrrole monomers supplied as vapor	555:588	We prepared electrically conductive nanoporous materials by coating nanoporous cellulose gels (NCG) with polypyrrole (PPy) nanoparticles, which were synthesized in situ from pyrrole monomers supplied as vapor.
24711342	5	13	dep	adhesion	993:1000	arg1	the					989:991	the	989:991	the	989:991	In vitro studies showed that the incorporation of PPy into an NCG enhances the adhesion and proliferation of PC12 cells.
24711342	5	14	theme	In vitro	914:921	arg1	studies					923:929	In vitro studies	914:929	In vitro studies	914:929	In vitro studies showed that the incorporation of PPy into an NCG enhances the adhesion and proliferation of PC12 cells.
24711342	2	15	theme	nanoporous	449:458	arg1	gels					470:473	nanoporous cellulose gels	449:473	nanoporous cellulose gels (NCG)	449:479	We prepared electrically conductive nanoporous materials by coating nanoporous cellulose gels (NCG) with polypyrrole (PPy) nanoparticles, which were synthesized in situ from pyrrole monomers supplied as vapor.
24711342	2	15	theme	nanoporous	449:458	arg1	NCG					476:478	NCG	476:478	NCG	476:478	We prepared electrically conductive nanoporous materials by coating nanoporous cellulose gels (NCG) with polypyrrole (PPy) nanoparticles, which were synthesized in situ from pyrrole monomers supplied as vapor.
24711342	0	16	theme	nerve	115:119	arg1	regeneration					121:132	nerve regeneration	115:132	nerve regeneration	115:132	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	3	17	theme	adsorption	743:752	arg1	0.41-0.53 g cm					714:727	0.41-0.53 g cm(-3)	714:731	0.41-0.53 g cm(-3)	714:731	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	3	17	theme	adsorption	743:752	arg1	areas					762:766	nitrogen adsorption surface areas	734:766	nitrogen adsorption surface areas of 264-303 m(2) g(-1)	734:788	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	4	18	theme	composite	833:841	arg1	hydrogels					843:851	The NCG/PPy composite hydrogels	821:851	The NCG/PPy composite hydrogels	821:851	The NCG/PPy composite hydrogels exhibited an electrical conductivity of up to 0.08 S cm(-1).
24711342	2	19	theme	polypyrrole	486:496	arg1	nanoparticles					504:516	polypyrrole (PPy) nanoparticles	486:516	polypyrrole (PPy) nanoparticles	486:516	We prepared electrically conductive nanoporous materials by coating nanoporous cellulose gels (NCG) with polypyrrole (PPy) nanoparticles, which were synthesized in situ from pyrrole monomers supplied as vapor.
24711342	1	20	theme	extracellular	207:219	arg1	matrix					221:226	extracellular matrix	207:226	extracellular matrix	207:226	Nanostructured conductive polymers can offer analogous environments for extracellular matrix and induce cellular responses by electric stimulation, however, such materials often lack mechanical strength and tend to collapse under small stresses.
24711342	3	21	theme	surface	754:760	arg1	0.41-0.53 g cm					714:727	0.41-0.53 g cm(-3)	714:731	0.41-0.53 g cm(-3)	714:731	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	3	21	theme	surface	754:760	arg1	areas					762:766	nitrogen adsorption surface areas	734:766	nitrogen adsorption surface areas of 264-303 m(2) g(-1)	734:788	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	3	22	theme	composite	613:621	arg1	hydrogels					623:631	The resulting NCG/PPy composite hydrogels	591:631	The resulting NCG/PPy composite hydrogels	591:631	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	0	23	theme	In	0:1	arg1	synthesis					8:16	In situ synthesis	0:16	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels	0:78	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	5	24	theme	cells	1028:1032	arg1	proliferation					1006:1018	proliferation	1006:1018	proliferation	1006:1018	In vitro studies showed that the incorporation of PPy into an NCG enhances the adhesion and proliferation of PC12 cells.
24711342	5	24	theme	cells	1028:1032	arg1	adhesion					993:1000	adhesion	993:1000	adhesion	993:1000	In vitro studies showed that the incorporation of PPy into an NCG enhances the adhesion and proliferation of PC12 cells.
24711342	1	25	theme	mechanical	318:327	arg1	strength					329:336	mechanical strength	318:336	mechanical strength	318:336	Nanostructured conductive polymers can offer analogous environments for extracellular matrix and induce cellular responses by electric stimulation, however, such materials often lack mechanical strength and tend to collapse under small stresses.
24711342	3	26	theme	resulting	595:603	arg1	hydrogels					623:631	The resulting NCG/PPy composite hydrogels	591:631	The resulting NCG/PPy composite hydrogels	591:631	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	4	27	dep	0.08 S cm	899:907	arg1	to					896:897	to	896:897	to	896:897	The NCG/PPy composite hydrogels exhibited an electrical conductivity of up to 0.08 S cm(-1).
24711342	3	28	theme	NCG/PPy	605:611	arg1	hydrogels					623:631	The resulting NCG/PPy composite hydrogels	591:631	The resulting NCG/PPy composite hydrogels	591:631	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	1	29	theme	cellular	239:246	arg1	responses					248:256	cellular responses	239:256	cellular responses	239:256	Nanostructured conductive polymers can offer analogous environments for extracellular matrix and induce cellular responses by electric stimulation, however, such materials often lack mechanical strength and tend to collapse under small stresses.
24711342	0	30	theme	conductive	28:37	arg1	aerogels					71:78	robust conductive cellulose/polypyrrole composite aerogels	21:78	robust conductive cellulose/polypyrrole composite aerogels	21:78	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	7	31	theme	many	1303:1306	arg1	others					1308:1313	many others	1303:1313	many others	1303:1313	These materials are promising candidates for applications in nerve regeneration, carbon capture, catalyst supports, and many others.
24711342	7	32	theme	carbon	1264:1269	arg1	capture					1271:1277	carbon capture	1264:1277	carbon capture	1264:1277	These materials are promising candidates for applications in nerve regeneration, carbon capture, catalyst supports, and many others.
24711342	0	33	theme	robust	21:26	arg1	aerogels					71:78	robust conductive cellulose/polypyrrole composite aerogels	21:78	robust conductive cellulose/polypyrrole composite aerogels	21:78	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	2	34	theme	cellulose	460:468	arg1	gels					470:473	nanoporous cellulose gels	449:473	nanoporous cellulose gels (NCG)	449:479	We prepared electrically conductive nanoporous materials by coating nanoporous cellulose gels (NCG) with polypyrrole (PPy) nanoparticles, which were synthesized in situ from pyrrole monomers supplied as vapor.
24711342	2	34	theme	cellulose	460:468	arg1	NCG					476:478	NCG	476:478	NCG	476:478	We prepared electrically conductive nanoporous materials by coating nanoporous cellulose gels (NCG) with polypyrrole (PPy) nanoparticles, which were synthesized in situ from pyrrole monomers supplied as vapor.
24711342	0	35	theme	composite	61:69	arg1	aerogels					71:78	robust conductive cellulose/polypyrrole composite aerogels	21:78	robust conductive cellulose/polypyrrole composite aerogels	21:78	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	4	36	theme	0.08 S cm	899:907	arg1	conductivity					877:888	an electrical conductivity	863:888	an electrical conductivity of up to 0.08 S cm(-1)	863:911	The NCG/PPy composite hydrogels exhibited an electrical conductivity of up to 0.08 S cm(-1).
24711342	3	37	with	drying	663:668	arg1	CO2					689:691	supercritical CO2	675:691	supercritical CO2	675:691	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	4	38	theme	NCG/PPy	825:831	arg1	hydrogels					843:851	The NCG/PPy composite hydrogels	821:851	The NCG/PPy composite hydrogels	821:851	The NCG/PPy composite hydrogels exhibited an electrical conductivity of up to 0.08 S cm(-1).
24711342	6	39	theme	longer	1109:1114	arg1	neurites					1116:1123	longer neurites	1109:1123	longer neurites	1109:1123	Electrical stimulation demonstrated that PC12 cells attached and extended longer neurites when cultured on NCG/PPy composite gels with DBSA dopant.
24711342	0	40	theme	cellulose/polypyrrole	39:59	arg1	aerogels					71:78	robust conductive cellulose/polypyrrole composite aerogels	21:78	robust conductive cellulose/polypyrrole composite aerogels	21:78	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	3	41	theme	g	784:784	arg1	0.41-0.53 g cm					714:727	0.41-0.53 g cm(-3)	714:731	0.41-0.53 g cm(-3)	714:731	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	3	41	theme	g	784:784	arg1	areas					762:766	nitrogen adsorption surface areas	734:766	nitrogen adsorption surface areas of 264-303 m(2) g(-1)	734:788	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	3	42	theme	0.41-0.53 g cm	714:727	arg1	strength					811:818	high mechanical strength	795:818	high mechanical strength	795:818	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	3	42	theme	0.41-0.53 g cm	714:727	arg1	density					703:709	a density	701:709	a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1),	701:789	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	5	43	theme	PC12	1023:1026	arg1	cells					1028:1032	PC12 cells	1023:1032	PC12 cells	1023:1032	In vitro studies showed that the incorporation of PPy into an NCG enhances the adhesion and proliferation of PC12 cells.
24711342	1	44	theme	Nanostructured	135:148	arg1	polymers					161:168	Nanostructured conductive polymers	135:168	Nanostructured conductive polymers	135:168	Nanostructured conductive polymers can offer analogous environments for extracellular matrix and induce cellular responses by electric stimulation, however, such materials often lack mechanical strength and tend to collapse under small stresses.
24711342	6	45	with	gels	1160:1163	arg1	dopant					1175:1180	DBSA dopant	1170:1180	DBSA dopant	1170:1180	Electrical stimulation demonstrated that PC12 cells attached and extended longer neurites when cultured on NCG/PPy composite gels with DBSA dopant.
24711342	7	46	from	applications	1228:1239	arg1	others					1308:1313	many others	1303:1313	many others	1303:1313	These materials are promising candidates for applications in nerve regeneration, carbon capture, catalyst supports, and many others.
24711342	7	46	from	applications	1228:1239	arg1	catalyst					1280:1287	catalyst supports	1280:1296	catalyst supports	1280:1296	These materials are promising candidates for applications in nerve regeneration, carbon capture, catalyst supports, and many others.
24711342	7	46	from	applications	1228:1239	arg1	regeneration					1250:1261	nerve regeneration	1244:1261	nerve regeneration	1244:1261	These materials are promising candidates for applications in nerve regeneration, carbon capture, catalyst supports, and many others.
24711342	7	46	from	applications	1228:1239	arg1	capture					1271:1277	carbon capture	1264:1277	carbon capture	1264:1277	These materials are promising candidates for applications in nerve regeneration, carbon capture, catalyst supports, and many others.
24711342	1	47	theme	conductive	150:159	arg1	polymers					161:168	Nanostructured conductive polymers	135:168	Nanostructured conductive polymers	135:168	Nanostructured conductive polymers can offer analogous environments for extracellular matrix and induce cellular responses by electric stimulation, however, such materials often lack mechanical strength and tend to collapse under small stresses.
24711342	6	48	theme	composite	1150:1158	arg1	gels					1160:1163	NCG/PPy composite gels	1142:1163	NCG/PPy composite gels with DBSA dopant	1142:1180	Electrical stimulation demonstrated that PC12 cells attached and extended longer neurites when cultured on NCG/PPy composite gels with DBSA dopant.
24711342	1	49	theme	electric	261:268	arg1	stimulation					270:280	electric stimulation	261:280	electric stimulation	261:280	Nanostructured conductive polymers can offer analogous environments for extracellular matrix and induce cellular responses by electric stimulation, however, such materials often lack mechanical strength and tend to collapse under small stresses.
24711342	6	50	theme	DBSA	1170:1173	arg1	dopant					1175:1180	DBSA dopant	1170:1180	DBSA dopant	1170:1180	Electrical stimulation demonstrated that PC12 cells attached and extended longer neurites when cultured on NCG/PPy composite gels with DBSA dopant.
24711342	3	51	theme	supercritical	675:687	arg1	CO2					689:691	supercritical CO2	675:691	supercritical CO2	675:691	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	3	52	theme	mechanical	800:809	arg1	strength					811:818	high mechanical strength	795:818	high mechanical strength	795:818	The resulting NCG/PPy composite hydrogels were converted to aerogels by drying with supercritical CO2, giving a density of 0.41-0.53 g cm(-3), nitrogen adsorption surface areas of 264-303 m(2) g(-1), and high mechanical strength.
24711342	7	53	theme	nerve	1244:1248	arg1	regeneration					1250:1261	nerve regeneration	1244:1261	nerve regeneration	1244:1261	These materials are promising candidates for applications in nerve regeneration, carbon capture, catalyst supports, and many others.
24711342	0	54	from	application	100:110	arg1	regeneration					121:132	nerve regeneration	115:132	nerve regeneration	115:132	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	2	55	theme	nanoporous	417:426	arg1	materials					428:436	electrically conductive nanoporous materials	393:436	electrically conductive nanoporous materials	393:436	We prepared electrically conductive nanoporous materials by coating nanoporous cellulose gels (NCG) with polypyrrole (PPy) nanoparticles, which were synthesized in situ from pyrrole monomers supplied as vapor.
24711342	6	56	theme	PC12	1076:1079	arg1	cells					1081:1085	PC12 cells	1076:1085	PC12 cells	1076:1085	Electrical stimulation demonstrated that PC12 cells attached and extended longer neurites when cultured on NCG/PPy composite gels with DBSA dopant.
24711342	0	57	theme	aerogels	71:78	arg1	synthesis					8:16	In situ synthesis	0:16	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels	0:78	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	0	57	theme	aerogels	71:78	arg1	application					100:110	their potential application	84:110	their potential application in nerve regeneration	84:132	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	1	58	theme	small	365:369	arg1	stresses					371:378	small stresses	365:378	small stresses	365:378	Nanostructured conductive polymers can offer analogous environments for extracellular matrix and induce cellular responses by electric stimulation, however, such materials often lack mechanical strength and tend to collapse under small stresses.
24711342	0	59	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration.
24711342	2	60	theme	conductive	406:415	arg1	materials					428:436	electrically conductive nanoporous materials	393:436	electrically conductive nanoporous materials	393:436	We prepared electrically conductive nanoporous materials by coating nanoporous cellulose gels (NCG) with polypyrrole (PPy) nanoparticles, which were synthesized in situ from pyrrole monomers supplied as vapor.
28320456	10	0	theme	varying	1799:1805	arg1	concentrations					1807:1820	varying concentrations	1799:1820	varying concentrations of both polymer and plasticizer	1799:1852	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	2	1	theme	thiol	330:334	arg1	modification					336:347	the thiol modification	326:347	the thiol modification of Arabinoxylan (ARX)	326:369	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	9	2	theme	mucoadhesive	1541:1552	arg1	11.53 ± 0.17 N					1563:1576	mucoadhesive strength 11.53 ± 0.17 N	1541:1576	mucoadhesive strength 11.53 ± 0.17 N	1541:1576	From the results, it was observed that the films were of satisfactory mechanical strength and mucoadhesiveness with folding endurance greater than 300 and mucoadhesive strength 11.53 ± 0.17 N, respectively.
28320456	10	3	contain	has	2022:2024	arg1	TARX					2017:2020	TARX	2017:2020	TARX	2017:2020	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	3	contain	has	2022:2024	arg2	impact					2035:2040	opposite impact	2026:2040	opposite impact	2026:2040	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	4	from	Effects	1788:1794	arg1	films					1884:1888	prepared mucoadhesive oral films	1857:1888	prepared mucoadhesive oral films	1857:1888	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	5	theme	polymer	1830:1836	arg1	concentrations					1807:1820	varying concentrations	1799:1820	varying concentrations of both polymer and plasticizer	1799:1852	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	3	6	theme	thiolated	496:504	arg1	arabinoxylan					506:517	thiolated arabinoxylan	496:517	thiolated arabinoxylan (TARX)	496:524	METHOD Synthesis of thiolated arabinoxylan (TARX) was accomplished by esterification of ARX with thioglycolic acid (TGA).
28320456	3	6	theme	thiolated	496:504	arg1	TARX					520:523	TARX	520:523	TARX	520:523	METHOD Synthesis of thiolated arabinoxylan (TARX) was accomplished by esterification of ARX with thioglycolic acid (TGA).
28320456	1	7	theme	drug	178:181	arg1	system					192:197	oral drug delivery system	173:197	oral drug delivery system	173:197	BACKGROUND Mucoadhesion is an important property that helps oral drug delivery system to remain attached with buccal mucosa and hence to improve the delivery of the drug.
28320456	10	8	dep	enhanced	1952:1959	arg1	can					1948:1950	can	1948:1950	can	1948:1950	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	9	theme	oral	1879:1882	arg1	films					1884:1888	prepared mucoadhesive oral films	1857:1888	prepared mucoadhesive oral films	1857:1888	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	6	10	theme	Prepared	871:878	arg1	formulations					885:896	Prepared film formulations	871:896	Prepared film formulations	871:896	Prepared film formulations were evaluated for mechanical strength, ex-vivo mucoadhesion, in-vitro drug release, ex-vivo drug permeation, surface morphology and drug contents.
28320456	11	11	theme	oral	2200:2203	arg1	films					2205:2209	controlled release mucoadhesive oral films	2168:2209	controlled release mucoadhesive oral films of TZN HCl	2168:2220	CONCLUSION In nutshell, TARX in combination with glycerolwas found to be suitable for the development of controlled release mucoadhesive oral films of TZN HCl.
28320456	10	12	dep	permeation	1705:1714	arg1	amount					1735:1740	78.90% cumulative amount	1717:1740	78.90% cumulative amount of permeated drug	1717:1758	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	12	dep	permeation	1705:1714	arg1	drug					1755:1758	permeated drug	1745:1758	permeated drug	1745:1758	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	13	theme	prepared	1857:1864	arg1	films					1884:1888	prepared mucoadhesive oral films	1857:1888	prepared mucoadhesive oral films	1857:1888	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	14	theme	drug	1679:1682	arg1	release					1684:1690	cumulative drug release	1668:1690	85.03% cumulative drug release	1661:1690	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	11	15	theme	release	2179:2185	arg1	films					2205:2209	controlled release mucoadhesive oral films	2168:2209	controlled release mucoadhesive oral films of TZN HCl	2168:2220	CONCLUSION In nutshell, TARX in combination with glycerolwas found to be suitable for the development of controlled release mucoadhesive oral films of TZN HCl.
28320456	3	16	theme	ARX	564:566	arg1	esterification					546:559	esterification	546:559	esterification of ARX with thioglycolic acid (TGA)	546:595	METHOD Synthesis of thiolated arabinoxylan (TARX) was accomplished by esterification of ARX with thioglycolic acid (TGA).
28320456	4	17	used	used	615:618	arg2	TARX					598:601	TARX	598:601	TARX	598:601	TARX was further used for the development of mucoadhesive oral films which were prepared by using a solvent casting technique.
28320456	5	18	theme	composite	794:802	arg1	TARX					829:832	selecting TARX	819:832	selecting TARX (X1)	819:837	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	18	theme	composite	794:802	arg1	CCRD					812:815	CCRD	812:815	CCRD	812:815	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	18	theme	composite	794:802	arg1	variables					860:868	variables	860:868	variables	860:868	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	18	theme	composite	794:802	arg1	design					804:809	central composite design	786:809	central composite design (CCRD)	786:816	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	18	theme	composite	794:802	arg1	glycerol					843:850	glycerol	843:850	glycerol (X2)	843:855	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	11	19	theme	controlled	2168:2177	arg1	films					2205:2209	controlled release mucoadhesive oral films	2168:2209	controlled release mucoadhesive oral films of TZN HCl	2168:2220	CONCLUSION In nutshell, TARX in combination with glycerolwas found to be suitable for the development of controlled release mucoadhesive oral films of TZN HCl.
28320456	4	20	theme	films	661:665	arg1	development					628:638	the development	624:638	the development of mucoadhesive oral films which were prepared by using a solvent casting technique	624:722	TARX was further used for the development of mucoadhesive oral films which were prepared by using a solvent casting technique.
28320456	10	21	theme	ex-vivo	1697:1703	arg1	permeation					1705:1714	ex-vivo permeation	1697:1714	ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films	1697:1888	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	22	theme	cumulative	1668:1677	arg1	release					1684:1690	cumulative drug release	1668:1690	85.03% cumulative drug release	1661:1690	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	8	23	theme	thiolation	1217:1226	arg1	claim					1197:1201	The claim	1193:1201	The claim of successful thiolation of ARX	1193:1233	The claim of successful thiolation of ARX was strengthened by the presence of 2809.003 ± 1.03 μmoles of thiol contents per gram of the polymer, which was determined by Ellman's reagent method.
28320456	3	24	theme	thioglycolic	573:584	arg1	TGA					592:594	TGA	592:594	TGA	592:594	METHOD Synthesis of thiolated arabinoxylan (TARX) was accomplished by esterification of ARX with thioglycolic acid (TGA).
28320456	3	24	theme	thioglycolic	573:584	arg1	acid					586:589	thioglycolic acid	573:589	thioglycolic acid (TGA)	573:595	METHOD Synthesis of thiolated arabinoxylan (TARX) was accomplished by esterification of ARX with thioglycolic acid (TGA).
28320456	5	25	theme	selecting	819:827	arg1	TARX					829:832	selecting TARX	819:832	selecting TARX (X1)	819:837	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	25	theme	selecting	819:827	arg1	design					804:809	central composite design	786:809	central composite design (CCRD)	786:816	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	25	theme	selecting	819:827	arg1	variables					860:868	variables	860:868	variables	860:868	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	25	theme	selecting	819:827	arg1	X1					835:836	X1	835:836	X1	835:836	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	25	theme	selecting	819:827	arg1	glycerol					843:850	glycerol	843:850	glycerol (X2)	843:855	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	6	26	theme	mechanical	917:926	arg1	strength					928:935	mechanical strength	917:935	mechanical strength	917:935	Prepared film formulations were evaluated for mechanical strength, ex-vivo mucoadhesion, in-vitro drug release, ex-vivo drug permeation, surface morphology and drug contents.
28320456	4	27	theme	mucoadhesive	643:654	arg1	films					661:665	mucoadhesive oral films	643:665	mucoadhesive oral films which were prepared by using a solvent casting technique	643:722	TARX was further used for the development of mucoadhesive oral films which were prepared by using a solvent casting technique.
28320456	10	28	theme	plasticizer	1842:1852	arg1	concentrations					1807:1820	varying concentrations	1799:1820	varying concentrations of both polymer and plasticizer	1799:1852	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	0	29	theme	controlled	69:78	arg1	films					106:110	controlled release mucoadhesive oral films	69:110	controlled release mucoadhesive oral films	69:110	Thiolation of arabinoxylan and its application in the fabrication of controlled release mucoadhesive oral films.
28320456	0	30	from	Thiolation	0:9	arg1	fabrication					54:64	the fabrication	50:64	the fabrication of controlled release mucoadhesive oral films	50:110	Thiolation of arabinoxylan and its application in the fabrication of controlled release mucoadhesive oral films.
28320456	4	31	theme	casting	706:712	arg1	technique					714:722	a solvent casting technique	696:722	a solvent casting technique	696:722	TARX was further used for the development of mucoadhesive oral films which were prepared by using a solvent casting technique.
28320456	0	32	theme	mucoadhesive	88:99	arg1	films					106:110	controlled release mucoadhesive oral films	69:110	controlled release mucoadhesive oral films	69:110	Thiolation of arabinoxylan and its application in the fabrication of controlled release mucoadhesive oral films.
28320456	7	33	dep	RESULTS	1046:1052	arg1	confirmed					1076:1084	confirmed	1076:1084	was confirmed by fourier transform infra-red spectroscopy (FTIR) as a peak related to thiol group appeared at 2516 cm-1	1072:1190	RESULTS Thiolation of ARX was confirmed by fourier transform infra-red spectroscopy (FTIR) as a peak related to thiol group appeared at 2516 cm-1.
28320456	0	34	theme	films	106:110	arg1	fabrication					54:64	the fabrication	50:64	the fabrication of controlled release mucoadhesive oral films	50:110	Thiolation of arabinoxylan and its application in the fabrication of controlled release mucoadhesive oral films.
28320456	6	35	theme	ex-vivo	983:989	arg1	permeation					996:1005	ex-vivo drug permeation	983:1005	ex-vivo drug permeation	983:1005	Prepared film formulations were evaluated for mechanical strength, ex-vivo mucoadhesion, in-vitro drug release, ex-vivo drug permeation, surface morphology and drug contents.
28320456	11	36	dep	CONCLUSION	2063:2072	arg1	nutshell					2077:2084	nutshell	2077:2084	nutshell	2077:2084	CONCLUSION In nutshell, TARX in combination with glycerolwas found to be suitable for the development of controlled release mucoadhesive oral films of TZN HCl.
28320456	8	37	theme	μmoles	1287:1292	arg1	presence					1259:1266	the presence	1255:1266	the presence of 2809.003 ± 1.03 μmoles of thiol contents per gram of the polymer, which was determined by Ellman's reagent method	1255:1383	The claim of successful thiolation of ARX was strengthened by the presence of 2809.003 ± 1.03 μmoles of thiol contents per gram of the polymer, which was determined by Ellman's reagent method.
28320456	2	38	theme	mucoadhesive	388:399	arg1	film					406:409	a mucoadhesive oral film	386:409	a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl)	386:473	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	0	39	theme	arabinoxylan	14:25	arg1	application					35:45	its application	31:45	its application in the fabrication of controlled release mucoadhesive oral films	31:110	Thiolation of arabinoxylan and its application in the fabrication of controlled release mucoadhesive oral films.
28320456	0	39	theme	arabinoxylan	14:25	arg1	Thiolation					0:9	Thiolation	0:9	Thiolation of arabinoxylan	0:25	Thiolation of arabinoxylan and its application in the fabrication of controlled release mucoadhesive oral films.
28320456	1	40	theme	drug	278:281	arg1	delivery					262:269	the delivery	258:269	the delivery of the drug	258:281	BACKGROUND Mucoadhesion is an important property that helps oral drug delivery system to remain attached with buccal mucosa and hence to improve the delivery of the drug.
28320456	9	41	theme	mechanical	1456:1465	arg1	strength					1467:1474	satisfactory mechanical strength	1443:1474	satisfactory mechanical strength	1443:1474	From the results, it was observed that the films were of satisfactory mechanical strength and mucoadhesiveness with folding endurance greater than 300 and mucoadhesive strength 11.53 ± 0.17 N, respectively.
28320456	6	42	theme	in-vitro	960:967	arg1	release					974:980	in-vitro drug release	960:980	in-vitro drug release	960:980	Prepared film formulations were evaluated for mechanical strength, ex-vivo mucoadhesion, in-vitro drug release, ex-vivo drug permeation, surface morphology and drug contents.
28320456	2	43	theme	tizanidine	440:449	arg1	hydrochloride					451:463	tizanidine hydrochloride	440:463	tizanidine hydrochloride (TZN HCl)	440:473	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	2	43	theme	tizanidine	440:449	arg1	HCl					470:472	TZN HCl	466:472	TZN HCl	466:472	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	12	44	theme	oral	2323:2326	arg1	film					2328:2331	fabricated oral film	2312:2331	fabricated oral film	2312:2331	Schematic diagram showing conversion of ARX to TARX, TARX to oral film and evaluation of fabricated oral film.
28320456	8	45	theme	thiol	1297:1301	arg1	contents					1303:1310	thiol contents	1297:1310	thiol contents per gram of the polymer, which was determined by Ellman's reagent method	1297:1383	The claim of successful thiolation of ARX was strengthened by the presence of 2809.003 ± 1.03 μmoles of thiol contents per gram of the polymer, which was determined by Ellman's reagent method.
28320456	7	46	theme	ARX	1068:1070	arg1	Thiolation					1054:1063	Thiolation	1054:1063	Thiolation of ARX	1054:1070	RESULTS Thiolation of ARX was confirmed by fourier transform infra-red spectroscopy (FTIR) as a peak related to thiol group appeared at 2516 cm-1.
28320456	12	47	theme	oral	2284:2287	arg1	film					2289:2292	oral film	2284:2292	oral film	2284:2292	Schematic diagram showing conversion of ARX to TARX, TARX to oral film and evaluation of fabricated oral film.
28320456	10	48	theme	permeated	1745:1753	arg1	drug					1755:1758	permeated drug	1745:1758	permeated drug	1745:1758	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	5	49	theme	films	744:748	arg1	Formulation					725:735	Formulation	725:735	Formulation of the films	725:748	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	10	50	theme	%	1722:1722	arg1	amount					1735:1740	78.90% cumulative amount	1717:1740	78.90% cumulative amount of permeated drug	1717:1758	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	50	theme	%	1722:1722	arg1	drug					1755:1758	permeated drug	1745:1758	permeated drug	1745:1758	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	51	theme	8 h.	1783:1786	arg1	Effects					1788:1794	8 h. Effects	1783:1794	8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films	1783:1888	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	52	theme	drug	1604:1607	arg1	retention					1609:1617	Reasonable drug retention	1593:1617	Reasonable drug retention	1593:1617	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	1	53	theme	buccal	223:228	arg1	mucosa					230:235	buccal mucosa	223:235	buccal mucosa	223:235	BACKGROUND Mucoadhesion is an important property that helps oral drug delivery system to remain attached with buccal mucosa and hence to improve the delivery of the drug.
28320456	11	54	theme	films	2205:2209	arg1	development					2153:2163	the development	2149:2163	the development of controlled release mucoadhesive oral films of TZN HCl	2149:2220	CONCLUSION In nutshell, TARX in combination with glycerolwas found to be suitable for the development of controlled release mucoadhesive oral films of TZN HCl.
28320456	8	55	theme	polymer	1328:1334	arg1	gram					1316:1319	gram	1316:1319	gram of the polymer, which was determined by Ellman's reagent method	1316:1383	The claim of successful thiolation of ARX was strengthened by the presence of 2809.003 ± 1.03 μmoles of thiol contents per gram of the polymer, which was determined by Ellman's reagent method.
28320456	3	56	theme	METHOD	476:481	arg1	Synthesis					483:491	METHOD Synthesis	476:491	METHOD Synthesis of thiolated arabinoxylan (TARX)	476:524	METHOD Synthesis of thiolated arabinoxylan (TARX) was accomplished by esterification of ARX with thioglycolic acid (TGA).
28320456	7	57	theme	thiol	1158:1162	arg1	group					1164:1168	thiol group	1158:1168	thiol group appeared at 2516 cm-1	1158:1190	RESULTS Thiolation of ARX was confirmed by fourier transform infra-red spectroscopy (FTIR) as a peak related to thiol group appeared at 2516 cm-1.
28320456	10	58	theme	concentrations	1807:1820	arg1	Effects					1788:1794	8 h. Effects	1783:1794	8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films	1783:1888	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	2	59	mod	modification	336:347	arg3	thiol					330:334	the thiol modification	326:347	the thiol modification of Arabinoxylan (ARX)	326:369	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	2	59	mod	modification	336:347	arg1	ARX					366:368	ARX	366:368	ARX	366:368	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	2	59	mod	modification	336:347	arg1	Arabinoxylan					352:363	Arabinoxylan	352:363	Arabinoxylan (ARX)	352:369	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	7	60	theme	related	1147:1153	arg1	spectroscopy					1117:1128	infra-red spectroscopy	1107:1128	infra-red spectroscopy (FTIR)	1107:1135	RESULTS Thiolation of ARX was confirmed by fourier transform infra-red spectroscopy (FTIR) as a peak related to thiol group appeared at 2516 cm-1.
28320456	7	60	theme	related	1147:1153	arg1	peak					1142:1145	a peak	1140:1145	a peak related to thiol group appeared at 2516 cm-1	1140:1190	RESULTS Thiolation of ARX was confirmed by fourier transform infra-red spectroscopy (FTIR) as a peak related to thiol group appeared at 2516 cm-1.
28320456	9	61	with	mucoadhesiveness	1480:1495	arg1	endurance					1510:1518	folding endurance	1502:1518	folding endurance greater than 300	1502:1535	From the results, it was observed that the films were of satisfactory mechanical strength and mucoadhesiveness with folding endurance greater than 300 and mucoadhesive strength 11.53 ± 0.17 N, respectively.
28320456	9	61	with	mucoadhesiveness	1480:1495	arg1	11.53 ± 0.17 N					1563:1576	mucoadhesive strength 11.53 ± 0.17 N	1541:1576	mucoadhesive strength 11.53 ± 0.17 N	1541:1576	From the results, it was observed that the films were of satisfactory mechanical strength and mucoadhesiveness with folding endurance greater than 300 and mucoadhesive strength 11.53 ± 0.17 N, respectively.
28320456	11	62	from	TARX	2087:2090	arg1	combination					2095:2105	combination	2095:2105	combination with glycerolwas	2095:2122	CONCLUSION In nutshell, TARX in combination with glycerolwas found to be suitable for the development of controlled release mucoadhesive oral films of TZN HCl.
28320456	3	63	theme	arabinoxylan	506:517	arg1	Synthesis					483:491	METHOD Synthesis	476:491	METHOD Synthesis of thiolated arabinoxylan (TARX)	476:524	METHOD Synthesis of thiolated arabinoxylan (TARX) was accomplished by esterification of ARX with thioglycolic acid (TGA).
28320456	9	64	theme	strength	1554:1561	arg1	11.53 ± 0.17 N					1563:1576	mucoadhesive strength 11.53 ± 0.17 N	1541:1576	mucoadhesive strength 11.53 ± 0.17 N	1541:1576	From the results, it was observed that the films were of satisfactory mechanical strength and mucoadhesiveness with folding endurance greater than 300 and mucoadhesive strength 11.53 ± 0.17 N, respectively.
28320456	8	65	theme	ARX	1231:1233	arg1	thiolation					1217:1226	successful thiolation	1206:1226	successful thiolation of ARX	1206:1233	The claim of successful thiolation of ARX was strengthened by the presence of 2809.003 ± 1.03 μmoles of thiol contents per gram of the polymer, which was determined by Ellman's reagent method.
28320456	12	66	theme	ARX	2263:2265	arg1	conversion					2249:2258	conversion	2249:2258	conversion of ARX to TARX, TARX to oral film and evaluation of fabricated oral film	2249:2331	Schematic diagram showing conversion of ARX to TARX, TARX to oral film and evaluation of fabricated oral film.
28320456	9	67	with	strength	1467:1474	arg1	endurance					1510:1518	folding endurance	1502:1518	folding endurance greater than 300	1502:1535	From the results, it was observed that the films were of satisfactory mechanical strength and mucoadhesiveness with folding endurance greater than 300 and mucoadhesive strength 11.53 ± 0.17 N, respectively.
28320456	9	67	with	strength	1467:1474	arg1	11.53 ± 0.17 N					1563:1576	mucoadhesive strength 11.53 ± 0.17 N	1541:1576	mucoadhesive strength 11.53 ± 0.17 N	1541:1576	From the results, it was observed that the films were of satisfactory mechanical strength and mucoadhesiveness with folding endurance greater than 300 and mucoadhesive strength 11.53 ± 0.17 N, respectively.
28320456	1	68	theme	oral	173:176	arg1	system					192:197	oral drug delivery system	173:197	oral drug delivery system	173:197	BACKGROUND Mucoadhesion is an important property that helps oral drug delivery system to remain attached with buccal mucosa and hence to improve the delivery of the drug.
28320456	10	69	theme	drug	2006:2009	arg1	dissolution					1965:1975	the dissolution	1961:1975	the dissolution as well as permeation of the drug	1961:2009	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	69	theme	drug	2006:2009	arg1	permeation					1988:1997	permeation	1988:1997	the dissolution as well as permeation of the drug	1961:2009	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	1	70	theme	delivery	183:190	arg1	system					192:197	oral drug delivery system	173:197	oral drug delivery system	173:197	BACKGROUND Mucoadhesion is an important property that helps oral drug delivery system to remain attached with buccal mucosa and hence to improve the delivery of the drug.
28320456	6	71	theme	film	880:883	arg1	formulations					885:896	Prepared film formulations	871:896	Prepared film formulations	871:896	Prepared film formulations were evaluated for mechanical strength, ex-vivo mucoadhesion, in-vitro drug release, ex-vivo drug permeation, surface morphology and drug contents.
28320456	11	72	theme	mucoadhesive	2187:2198	arg1	films					2205:2209	controlled release mucoadhesive oral films	2168:2209	controlled release mucoadhesive oral films of TZN HCl	2168:2220	CONCLUSION In nutshell, TARX in combination with glycerolwas found to be suitable for the development of controlled release mucoadhesive oral films of TZN HCl.
28320456	10	73	theme	mucoadhesive	1866:1877	arg1	films					1884:1888	prepared mucoadhesive oral films	1857:1888	prepared mucoadhesive oral films	1857:1888	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	2	74	theme	current	288:294	arg1	study					296:300	The current study	284:300	The current study	284:300	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	12	75	theme	Schematic	2223:2231	arg1	diagram					2233:2239	Schematic diagram	2223:2239	Schematic diagram	2223:2239	Schematic diagram showing conversion of ARX to TARX, TARX to oral film and evaluation of fabricated oral film.
28320456	10	76	theme	opposite	2026:2033	arg1	impact					2035:2040	opposite impact	2026:2040	opposite impact	2026:2040	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	77	theme	78.90	1717:1721	arg1	%					1722:1722	%	1722:1722	%	1722:1722	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	5	78	theme	central	786:792	arg1	TARX					829:832	selecting TARX	819:832	selecting TARX (X1)	819:837	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	78	theme	central	786:792	arg1	CCRD					812:815	CCRD	812:815	CCRD	812:815	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	78	theme	central	786:792	arg1	variables					860:868	variables	860:868	variables	860:868	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	78	theme	central	786:792	arg1	design					804:809	central composite design	786:809	central composite design (CCRD)	786:816	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	5	78	theme	central	786:792	arg1	glycerol					843:850	glycerol	843:850	glycerol (X2)	843:855	Formulation of the films was designed and optimized by using central composite design (CCRD), selecting TARX (X1) and glycerol (X2) as variables.
28320456	10	79	theme	permeation	1705:1714	arg1	studies					1761:1767	ex-vivo permeation (78.90% cumulative amount of permeated drug) studies	1697:1767	ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films	1697:1888	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	1	80	theme	BACKGROUND	113:122	arg1	property					153:160	an important property	140:160	an important property that helps oral drug delivery system to remain attached with buccal mucosa and hence to improve the delivery of the drug	140:281	BACKGROUND Mucoadhesion is an important property that helps oral drug delivery system to remain attached with buccal mucosa and hence to improve the delivery of the drug.
28320456	1	80	theme	BACKGROUND	113:122	arg1	Mucoadhesion					124:135	BACKGROUND Mucoadhesion	113:135	BACKGROUND Mucoadhesion	113:135	BACKGROUND Mucoadhesion is an important property that helps oral drug delivery system to remain attached with buccal mucosa and hence to improve the delivery of the drug.
28320456	4	81	theme	oral	656:659	arg1	films					661:665	mucoadhesive oral films	643:665	mucoadhesive oral films which were prepared by using a solvent casting technique	643:722	TARX was further used for the development of mucoadhesive oral films which were prepared by using a solvent casting technique.
28320456	6	82	theme	ex-vivo	938:944	arg1	mucoadhesion					946:957	ex-vivo mucoadhesion	938:957	ex-vivo mucoadhesion	938:957	Prepared film formulations were evaluated for mechanical strength, ex-vivo mucoadhesion, in-vitro drug release, ex-vivo drug permeation, surface morphology and drug contents.
28320456	8	83	theme	reagent	1370:1376	arg1	method					1378:1383	Ellman's reagent method	1361:1383	Ellman's reagent method	1361:1383	The claim of successful thiolation of ARX was strengthened by the presence of 2809.003 ± 1.03 μmoles of thiol contents per gram of the polymer, which was determined by Ellman's reagent method.
28320456	11	84	with	combination	2095:2105	arg1	glycerolwas					2112:2122	glycerolwas	2112:2122	glycerolwas	2112:2122	CONCLUSION In nutshell, TARX in combination with glycerolwas found to be suitable for the development of controlled release mucoadhesive oral films of TZN HCl.
28320456	10	85	theme	cumulative	1724:1733	arg1	amount					1735:1740	78.90% cumulative amount	1717:1740	78.90% cumulative amount of permeated drug	1717:1758	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	85	theme	cumulative	1724:1733	arg1	drug					1755:1758	permeated drug	1745:1758	permeated drug	1745:1758	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	1	86	theme	important	143:151	arg1	property					153:160	an important property	140:160	an important property that helps oral drug delivery system to remain attached with buccal mucosa and hence to improve the delivery of the drug	140:281	BACKGROUND Mucoadhesion is an important property that helps oral drug delivery system to remain attached with buccal mucosa and hence to improve the delivery of the drug.
28320456	1	86	theme	important	143:151	arg1	Mucoadhesion					124:135	BACKGROUND Mucoadhesion	113:135	BACKGROUND Mucoadhesion	113:135	BACKGROUND Mucoadhesion is an important property that helps oral drug delivery system to remain attached with buccal mucosa and hence to improve the delivery of the drug.
28320456	8	87	theme	successful	1206:1215	arg1	thiolation					1217:1226	successful thiolation	1206:1226	successful thiolation of ARX	1206:1233	The claim of successful thiolation of ARX was strengthened by the presence of 2809.003 ± 1.03 μmoles of thiol contents per gram of the polymer, which was determined by Ellman's reagent method.
28320456	3	88	with	esterification	546:559	arg1	TGA					592:594	TGA	592:594	TGA	592:594	METHOD Synthesis of thiolated arabinoxylan (TARX) was accomplished by esterification of ARX with thioglycolic acid (TGA).
28320456	3	88	with	esterification	546:559	arg1	acid					586:589	thioglycolic acid	573:589	thioglycolic acid (TGA)	573:595	METHOD Synthesis of thiolated arabinoxylan (TARX) was accomplished by esterification of ARX with thioglycolic acid (TGA).
28320456	0	89	theme	release	80:86	arg1	films					106:110	controlled release mucoadhesive oral films	69:110	controlled release mucoadhesive oral films	69:110	Thiolation of arabinoxylan and its application in the fabrication of controlled release mucoadhesive oral films.
28320456	2	90	theme	hydrochloride	451:463	arg1	delivery					428:435	the improved delivery	415:435	the improved delivery of tizanidine hydrochloride (TZN HCl)	415:473	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	10	91	dep	%	1666:1666	arg1	release					1684:1690	cumulative drug release	1668:1690	85.03% cumulative drug release	1661:1690	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	0	92	theme	oral	101:104	arg1	films					106:110	controlled release mucoadhesive oral films	69:110	controlled release mucoadhesive oral films	69:110	Thiolation of arabinoxylan and its application in the fabrication of controlled release mucoadhesive oral films.
28320456	7	93	dep	fourier	1089:1095	arg1	transform					1097:1105	transform	1097:1105	transform infra-red spectroscopy (FTIR) as a peak related to thiol group appeared at 2516 cm-1	1097:1190	RESULTS Thiolation of ARX was confirmed by fourier transform infra-red spectroscopy (FTIR) as a peak related to thiol group appeared at 2516 cm-1.
28320456	4	94	theme	solvent	698:704	arg1	technique					714:722	a solvent casting technique	696:722	a solvent casting technique	696:722	TARX was further used for the development of mucoadhesive oral films which were prepared by using a solvent casting technique.
28320456	10	95	theme	Reasonable	1593:1602	arg1	retention					1609:1617	Reasonable drug retention	1593:1617	Reasonable drug retention	1593:1617	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	6	96	theme	drug	991:994	arg1	permeation					996:1005	ex-vivo drug permeation	983:1005	ex-vivo drug permeation	983:1005	Prepared film formulations were evaluated for mechanical strength, ex-vivo mucoadhesion, in-vitro drug release, ex-vivo drug permeation, surface morphology and drug contents.
28320456	0	97	from	application	35:45	arg1	fabrication					54:64	the fabrication	50:64	the fabrication of controlled release mucoadhesive oral films	50:110	Thiolation of arabinoxylan and its application in the fabrication of controlled release mucoadhesive oral films.
28320456	12	98	theme	fabricated	2312:2321	arg1	film					2328:2331	fabricated oral film	2312:2331	fabricated oral film	2312:2331	Schematic diagram showing conversion of ARX to TARX, TARX to oral film and evaluation of fabricated oral film.
28320456	9	99	theme	satisfactory	1443:1454	arg1	strength					1467:1474	satisfactory mechanical strength	1443:1474	satisfactory mechanical strength	1443:1474	From the results, it was observed that the films were of satisfactory mechanical strength and mucoadhesiveness with folding endurance greater than 300 and mucoadhesive strength 11.53 ± 0.17 N, respectively.
28320456	6	100	theme	drug	969:972	arg1	release					974:980	in-vitro drug release	960:980	in-vitro drug release	960:980	Prepared film formulations were evaluated for mechanical strength, ex-vivo mucoadhesion, in-vitro drug release, ex-vivo drug permeation, surface morphology and drug contents.
28320456	2	101	theme	oral	401:404	arg1	film					406:409	a mucoadhesive oral film	386:409	a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl)	386:473	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	12	102	theme	film	2328:2331	arg1	evaluation					2298:2307	evaluation	2298:2307	evaluation of fabricated oral film	2298:2331	Schematic diagram showing conversion of ARX to TARX, TARX to oral film and evaluation of fabricated oral film.
28320456	12	102	theme	film	2328:2331	arg1	TARX					2276:2279	TARX	2276:2279	TARX to oral film	2276:2292	Schematic diagram showing conversion of ARX to TARX, TARX to oral film and evaluation of fabricated oral film.
28320456	12	102	theme	film	2328:2331	arg1	TARX					2270:2273	TARX	2270:2273	TARX	2270:2273	Schematic diagram showing conversion of ARX to TARX, TARX to oral film and evaluation of fabricated oral film.
28320456	2	103	theme	Arabinoxylan	352:363	arg1	modification					336:347	the thiol modification	326:347	the thiol modification of Arabinoxylan (ARX)	326:369	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	10	104	theme	drug	1755:1758	arg1	amount					1735:1740	78.90% cumulative amount	1717:1740	78.90% cumulative amount of permeated drug	1717:1758	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	104	theme	drug	1755:1758	arg1	drug					1755:1758	permeated drug	1745:1758	permeated drug	1745:1758	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	7	105	theme	infra-red	1107:1115	arg1	spectroscopy					1117:1128	infra-red spectroscopy	1107:1128	infra-red spectroscopy (FTIR)	1107:1135	RESULTS Thiolation of ARX was confirmed by fourier transform infra-red spectroscopy (FTIR) as a peak related to thiol group appeared at 2516 cm-1.
28320456	7	105	theme	infra-red	1107:1115	arg1	peak					1142:1145	a peak	1140:1145	a peak related to thiol group appeared at 2516 cm-1	1140:1190	RESULTS Thiolation of ARX was confirmed by fourier transform infra-red spectroscopy (FTIR) as a peak related to thiol group appeared at 2516 cm-1.
28320456	7	105	theme	infra-red	1107:1115	arg1	FTIR					1131:1134	FTIR	1131:1134	FTIR	1131:1134	RESULTS Thiolation of ARX was confirmed by fourier transform infra-red spectroscopy (FTIR) as a peak related to thiol group appeared at 2516 cm-1.
28320456	2	106	theme	improved	419:426	arg1	delivery					428:435	the improved delivery	415:435	the improved delivery of tizanidine hydrochloride (TZN HCl)	415:473	The current study was designed to achieve the thiol modification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine hydrochloride (TZN HCl).
28320456	8	107	theme	contents	1303:1310	arg1	μmoles					1287:1292	2809.003 ± 1.03 μmoles	1271:1292	2809.003 ± 1.03 μmoles of thiol contents per gram of the polymer, which was determined by Ellman's reagent method	1271:1383	The claim of successful thiolation of ARX was strengthened by the presence of 2809.003 ± 1.03 μmoles of thiol contents per gram of the polymer, which was determined by Ellman's reagent method.
28320456	9	108	theme	folding	1502:1508	arg1	endurance					1510:1518	folding endurance	1502:1518	folding endurance greater than 300	1502:1535	From the results, it was observed that the films were of satisfactory mechanical strength and mucoadhesiveness with folding endurance greater than 300 and mucoadhesive strength 11.53 ± 0.17 N, respectively.
28320456	6	109	theme	drug	1031:1034	arg1	contents					1036:1043	drug contents	1031:1043	drug contents	1031:1043	Prepared film formulations were evaluated for mechanical strength, ex-vivo mucoadhesion, in-vitro drug release, ex-vivo drug permeation, surface morphology and drug contents.
28320456	11	110	theme	HCl	2218:2220	arg1	films					2205:2209	controlled release mucoadhesive oral films	2168:2209	controlled release mucoadhesive oral films of TZN HCl	2168:2220	CONCLUSION In nutshell, TARX in combination with glycerolwas found to be suitable for the development of controlled release mucoadhesive oral films of TZN HCl.
28320456	6	111	theme	surface	1008:1014	arg1	morphology					1016:1025	surface morphology	1008:1025	surface morphology	1008:1025	Prepared film formulations were evaluated for mechanical strength, ex-vivo mucoadhesion, in-vitro drug release, ex-vivo drug permeation, surface morphology and drug contents.
28320456	10	112	theme	in-vitro	1639:1646	arg1	%					1666:1666	85.03% cumulative drug release	1661:1690	85.03% cumulative drug release	1661:1690	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
28320456	10	112	theme	in-vitro	1639:1646	arg1	dissolution					1648:1658	in-vitro dissolution	1639:1658	in-vitro dissolution (85.03% cumulative drug release)	1639:1691	Reasonable drug retention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90% cumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer and plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can enhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.
27522024	8	0	theme	consortia	1591:1599	arg1	composition					1572:1582	the composition	1568:1582	the composition of the consortia	1568:1599	According to Illumina amplicon sequencing of 16S rRNA genes, the composition of the consortia underwent distinct shifts in concert with HNC versus microcrystalline cellulose degradation.
27522024	3	1	theme	microbial	548:556	arg1	communities					558:568	microbial communities	548:568	microbial communities	548:568	Given its distinction in terms of size and chemical and physical properties, nanocellulose could potentially resist biodegradation and/or pose a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments.
27522024	6	2	theme	decay	1087:1091	arg1	constants					1093:1101	1st order decay constants	1077:1101	1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium	1077:1304	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	5	3	theme	glucose	995:1001	arg1	measurement					970:980	measurement	970:980	measurement of liberated glucose	970:1001	Equivalent cellulose masses were dosed and monitored with time by measurement of liberated glucose.
27522024	6	4	dep	cellulose	1066:1074	arg1	constants					1093:1101	1st order decay constants	1077:1101	1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium	1077:1304	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	6	5	theme	AD	1192:1193	arg1	consortium					1195:1204	the AD consortium	1188:1204	the AD consortium	1188:1204	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	8	6	theme	distinct	1611:1618	arg1	shifts					1620:1625	distinct shifts	1611:1625	distinct shifts	1611:1625	According to Illumina amplicon sequencing of 16S rRNA genes, the composition of the consortia underwent distinct shifts in concert with HNC versus microcrystalline cellulose degradation.
27522024	6	7	theme	order	1081:1085	arg1	constants					1093:1101	1st order decay constants	1077:1101	1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium	1077:1304	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	7	8	theme	total	1318:1322	arg1	rRNA					1312:1315	16S rRNA	1308:1315	16S rRNA	1308:1315	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	7	8	theme	total	1318:1322	arg1	bacteria					1324:1331	total bacteria	1318:1331	total bacteria	1318:1331	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	8	9	theme	Illumina	1520:1527	arg1	sequencing					1538:1547	Illumina amplicon sequencing	1520:1547	Illumina amplicon sequencing of 16S rRNA genes	1520:1565	According to Illumina amplicon sequencing of 16S rRNA genes, the composition of the consortia underwent distinct shifts in concert with HNC versus microcrystalline cellulose degradation.
27522024	9	10	theme	nano-size	1779:1787	arg1	range					1789:1793	the nano-size range	1775:1793	the nano-size range	1775:1793	This study demonstrates that the biodegradation of cellulose is not inhibited in the nano-size range, particularly in the crystalline form, though the microbes and pathways involved likely differ.
27522024	4	11	theme	digester	865:872	arg1	AD					875:876	anaerobic digester (AD) and wetland (W) inocula	855:901	AD	875:876	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	6	12	theme	1st	1077:1079	arg1	constants					1093:1101	1st order decay constants	1077:1101	1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium	1077:1304	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	2	13	theme	communities	308:318	arg1	void					196:199	a void	194:199	a void of knowledge regarding the environmental fate of nanocellulose	194:262	However, there is a void of knowledge regarding the environmental fate of nanocellulose and the response of environmental microbial communities that are historically adapted to non-nano cellulose forms.
27522024	2	13	theme	communities	308:318	arg1	response					272:279	the response	268:279	the response of environmental microbial communities that are historically adapted to non-nano cellulose forms	268:376	However, there is a void of knowledge regarding the environmental fate of nanocellulose and the response of environmental microbial communities that are historically adapted to non-nano cellulose forms.
27522024	3	14	theme	receiving	604:612	arg1	environments					614:625	receiving environments	604:625	receiving environments	604:625	Given its distinction in terms of size and chemical and physical properties, nanocellulose could potentially resist biodegradation and/or pose a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments.
27522024	3	15	theme	xenobiotic	524:533	arg1	influence					535:543	a xenobiotic influence	522:543	a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments	522:625	Given its distinction in terms of size and chemical and physical properties, nanocellulose could potentially resist biodegradation and/or pose a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments.
27522024	5	16	theme	liberated	985:993	arg1	glucose					995:1001	liberated glucose	985:1001	liberated glucose	985:1001	Equivalent cellulose masses were dosed and monitored with time by measurement of liberated glucose.
27522024	2	17	theme	microbial	298:306	arg1	communities					308:318	environmental microbial communities	284:318	environmental microbial communities that are historically adapted to non-nano cellulose forms	284:376	However, there is a void of knowledge regarding the environmental fate of nanocellulose and the response of environmental microbial communities that are historically adapted to non-nano cellulose forms.
27522024	9	18	dep	microbes	1845:1852	arg1	the					1841:1843	the	1841:1843	the	1841:1843	This study demonstrates that the biodegradation of cellulose is not inhibited in the nano-size range, particularly in the crystalline form, though the microbes and pathways involved likely differ.
27522024	8	19	with	concert	1630:1636	arg1	cellulose					1671:1679	microcrystalline cellulose	1654:1679	microcrystalline cellulose	1654:1679	According to Illumina amplicon sequencing of 16S rRNA genes, the composition of the consortia underwent distinct shifts in concert with HNC versus microcrystalline cellulose degradation.
27522024	8	19	with	concert	1630:1636	arg1	HNC					1643:1645	HNC	1643:1645	HNC	1643:1645	According to Illumina amplicon sequencing of 16S rRNA genes, the composition of the consortia underwent distinct shifts in concert with HNC versus microcrystalline cellulose degradation.
27522024	2	20	theme	environmental	284:296	arg1	communities					308:318	environmental microbial communities	284:318	environmental microbial communities that are historically adapted to non-nano cellulose forms	284:376	However, there is a void of knowledge regarding the environmental fate of nanocellulose and the response of environmental microbial communities that are historically adapted to non-nano cellulose forms.
27522024	3	21	theme	size	413:416	arg1	terms					404:408	terms	404:408	terms of size and chemical and physical properties	404:453	Given its distinction in terms of size and chemical and physical properties, nanocellulose could potentially resist biodegradation and/or pose a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments.
27522024	6	22	theme	W	1293:1293	arg1	consortium					1295:1304	the W consortium	1289:1304	the W consortium	1289:1304	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	6	23	dep	constants	1093:1101	arg1	0.58 ± 0.05 wk-1					1237:1252	0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1	1207:1252	1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium	1077:1304	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	6	23	dep	constants	1093:1101	arg1	0.62 ± 0.08 wk-1					1104:1119	0.62 ± 0.08 wk-1	1104:1119	1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium	1077:1304	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	7	24	theme	16S	1308:1310	arg1	rRNA					1312:1315	16S rRNA	1308:1315	16S rRNA	1308:1315	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	7	24	theme	16S	1308:1310	arg1	bacteria					1324:1331	total bacteria	1318:1331	total bacteria	1318:1331	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	8	25	theme	microcrystalline	1654:1669	arg1	cellulose					1671:1679	microcrystalline cellulose	1654:1679	microcrystalline cellulose	1654:1679	According to Illumina amplicon sequencing of 16S rRNA genes, the composition of the consortia underwent distinct shifts in concert with HNC versus microcrystalline cellulose degradation.
27522024	4	26	theme	cellulose-degrading	792:810	arg1	consortia					822:830	two distinct anaerobic cellulose-degrading microbial consortia	769:830	two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula	769:901	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	3	27	from	influence	535:543	arg1	communities					558:568	microbial communities	548:568	microbial communities	548:568	Given its distinction in terms of size and chemical and physical properties, nanocellulose could potentially resist biodegradation and/or pose a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments.
27522024	3	27	from	influence	535:543	arg1	environments					614:625	receiving environments	604:625	receiving environments	604:625	Given its distinction in terms of size and chemical and physical properties, nanocellulose could potentially resist biodegradation and/or pose a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments.
27522024	6	28	theme	microcrystalline	1157:1172	arg1	cellulose					1174:1182	microcrystalline cellulose	1157:1182	microcrystalline cellulose	1157:1182	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	0	29	theme	cellulose	34:42	arg1	Biodegradation					0:13	Biodegradation	0:13	Biodegradation of nanocrystalline cellulose by two environmentally-relevant consortia.	0:85	Biodegradation of nanocrystalline cellulose by two environmentally-relevant consortia.
27522024	6	30	theme	microcrystalline	1049:1064	arg1	cellulose					1066:1074	microcrystalline cellulose	1049:1074	microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium)	1049:1305	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	8	31	theme	genes	1561:1565	arg1	sequencing					1538:1547	Illumina amplicon sequencing	1520:1547	Illumina amplicon sequencing of 16S rRNA genes	1520:1565	According to Illumina amplicon sequencing of 16S rRNA genes, the composition of the consortia underwent distinct shifts in concert with HNC versus microcrystalline cellulose degradation.
27522024	5	32	theme	Equivalent	904:913	arg1	masses					925:930	Equivalent cellulose masses	904:930	Equivalent cellulose masses	904:930	Equivalent cellulose masses were dosed and monitored with time by measurement of liberated glucose.
27522024	0	33	theme	nanocrystalline	18:32	arg1	cellulose					34:42	nanocrystalline cellulose	18:42	nanocrystalline cellulose	18:42	Biodegradation of nanocrystalline cellulose by two environmentally-relevant consortia.
27522024	8	34	theme	rRNA	1556:1559	arg1	genes					1561:1565	16S rRNA genes	1552:1565	16S rRNA genes	1552:1565	According to Illumina amplicon sequencing of 16S rRNA genes, the composition of the consortia underwent distinct shifts in concert with HNC versus microcrystalline cellulose degradation.
27522024	7	35	theme	hydrolase	1355:1363	arg1	family					1370:1375	glycoside hydrolase gene family 48	1345:1378	glycoside hydrolase gene family 48	1345:1378	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	5	36	theme	cellulose	915:923	arg1	masses					925:930	Equivalent cellulose masses	904:930	Equivalent cellulose masses	904:930	Equivalent cellulose masses were dosed and monitored with time by measurement of liberated glucose.
27522024	3	37	theme	physical	435:442	arg1	properties					444:453	chemical and physical properties	422:453	chemical and physical properties	422:453	Given its distinction in terms of size and chemical and physical properties, nanocellulose could potentially resist biodegradation and/or pose a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments.
27522024	4	38	theme	distinct	773:780	arg1	consortia					822:830	two distinct anaerobic cellulose-degrading microbial consortia	769:830	two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula	769:901	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	7	39	theme	gene	1365:1368	arg1	family					1370:1375	glycoside hydrolase gene family 48	1345:1378	glycoside hydrolase gene family 48	1345:1378	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	2	40	theme	nanocellulose	250:262	arg1	fate					242:245	the environmental fate	224:245	the environmental fate of nanocellulose	224:262	However, there is a void of knowledge regarding the environmental fate of nanocellulose and the response of environmental microbial communities that are historically adapted to non-nano cellulose forms.
27522024	7	41	theme	cellulose-degrading	1395:1413	arg1	potential					1415:1423	cellulose-degrading potential	1395:1423	cellulose-degrading potential	1395:1423	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	3	42	theme	properties	444:453	arg1	terms					404:408	terms	404:408	terms of size and chemical and physical properties	404:453	Given its distinction in terms of size and chemical and physical properties, nanocellulose could potentially resist biodegradation and/or pose a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments.
27522024	6	43	theme	faster	1032:1037	arg1	rate					1039:1042	slightly faster rate	1023:1042	slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium)	1023:1305	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	3	44	from	distinction	389:399	arg1	terms					404:408	terms	404:408	terms of size and chemical and physical properties	404:453	Given its distinction in terms of size and chemical and physical properties, nanocellulose could potentially resist biodegradation and/or pose a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments.
27522024	4	45	theme	W	892:892	arg1	inocula					895:901	anaerobic digester (AD) and wetland (W) inocula	855:901	inocula	895:901	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	7	46	theme	potential	1415:1423	arg1	indicative					1381:1390	indicative	1381:1390	indicative	1381:1390	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	3	47	theme	chemical	422:429	arg1	properties					444:453	chemical and physical properties	422:453	chemical and physical properties	422:453	Given its distinction in terms of size and chemical and physical properties, nanocellulose could potentially resist biodegradation and/or pose a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments.
27522024	9	48	from	range	1789:1793	arg1	form					1828:1831	the crystalline form	1812:1831	the crystalline form	1812:1831	This study demonstrates that the biodegradation of cellulose is not inhibited in the nano-size range, particularly in the crystalline form, though the microbes and pathways involved likely differ.
27522024	4	49	theme	anaerobic	855:863	arg1	AD					875:876	anaerobic digester (AD) and wetland (W) inocula	855:901	AD	875:876	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	7	50	theme	HNC	1473:1475	arg1	condition					1477:1485	the HNC condition	1469:1485	the HNC condition for both consortia	1469:1504	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	4	51	theme	anaerobic	782:790	arg1	consortia					822:830	two distinct anaerobic cellulose-degrading microbial consortia	769:830	two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula	769:901	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	0	52	theme	environmentally-relevant	51:74	arg1	consortia					76:84	two environmentally-relevant consortia	47:84	two environmentally-relevant consortia	47:84	Biodegradation of nanocrystalline cellulose by two environmentally-relevant consortia.
27522024	9	53	theme	crystalline	1816:1826	arg1	form					1828:1831	the crystalline form	1812:1831	the crystalline form	1812:1831	This study demonstrates that the biodegradation of cellulose is not inhibited in the nano-size range, particularly in the crystalline form, though the microbes and pathways involved likely differ.
27522024	2	54	theme	environmental	228:240	arg1	fate					242:245	the environmental fate	224:245	the environmental fate of nanocellulose	224:262	However, there is a void of knowledge regarding the environmental fate of nanocellulose and the response of environmental microbial communities that are historically adapted to non-nano cellulose forms.
27522024	7	55	theme	indicative	1381:1390	arg1	family					1370:1375	glycoside hydrolase gene family 48	1345:1378	glycoside hydrolase gene family 48	1345:1378	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	1	56	theme	versatile	137:145	arg1	properties					147:156	its versatile properties	133:156	its versatile properties	133:156	Nanocellulose is growing in popularity due to its versatile properties and applications.
27522024	8	57	theme	16S	1552:1554	arg1	genes					1561:1565	16S rRNA genes	1552:1565	16S rRNA genes	1552:1565	According to Illumina amplicon sequencing of 16S rRNA genes, the composition of the consortia underwent distinct shifts in concert with HNC versus microcrystalline cellulose degradation.
27522024	7	58	dep	genes	1426:1430	arg1	family					1370:1375	glycoside hydrolase gene family 48	1345:1378	glycoside hydrolase gene family 48	1345:1378	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	9	59	theme	cellulose	1745:1753	arg1	biodegradation					1727:1740	the biodegradation	1723:1740	the biodegradation of cellulose	1723:1753	This study demonstrates that the biodegradation of cellulose is not inhibited in the nano-size range, particularly in the crystalline form, though the microbes and pathways involved likely differ.
27522024	8	60	theme	amplicon	1529:1536	arg1	sequencing					1538:1547	Illumina amplicon sequencing	1520:1547	Illumina amplicon sequencing of 16S rRNA genes	1520:1565	According to Illumina amplicon sequencing of 16S rRNA genes, the composition of the consortia underwent distinct shifts in concert with HNC versus microcrystalline cellulose degradation.
27522024	4	61	theme	microcrystalline	736:751	arg1	cellulose					753:761	microcrystalline cellulose	736:761	microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula	736:901	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	2	62	theme	cellulose	362:370	arg1	forms					372:376	non-nano cellulose forms	353:376	non-nano cellulose forms	353:376	However, there is a void of knowledge regarding the environmental fate of nanocellulose and the response of environmental microbial communities that are historically adapted to non-nano cellulose forms.
27522024	6	63	theme	HNCversus	1227:1235	arg1	0.58 ± 0.05 wk-1					1237:1252	0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1	1207:1252	1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium	1077:1304	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	4	64	theme	hydrolyzed	667:676	arg1	HNC					705:707	HNC	705:707	HNC	705:707	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	4	64	theme	hydrolyzed	667:676	arg1	cellulose					694:702	H2SO4 hydrolyzed nanocrystalline cellulose	661:702	H2SO4 hydrolyzed nanocrystalline cellulose (HNC)	661:708	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	2	65	theme	knowledge	204:212	arg1	void					196:199	a void	194:199	a void of knowledge regarding the environmental fate of nanocellulose	194:262	However, there is a void of knowledge regarding the environmental fate of nanocellulose and the response of environmental microbial communities that are historically adapted to non-nano cellulose forms.
27522024	2	65	theme	knowledge	204:212	arg1	response					272:279	the response	268:279	the response of environmental microbial communities that are historically adapted to non-nano cellulose forms	268:376	However, there is a void of knowledge regarding the environmental fate of nanocellulose and the response of environmental microbial communities that are historically adapted to non-nano cellulose forms.
27522024	4	66	theme	microbial	812:820	arg1	consortia					822:830	two distinct anaerobic cellulose-degrading microbial consortia	769:830	two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula	769:901	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	2	67	theme	non-nano	353:360	arg1	forms					372:376	non-nano cellulose forms	353:376	non-nano cellulose forms	353:376	However, there is a void of knowledge regarding the environmental fate of nanocellulose and the response of environmental microbial communities that are historically adapted to non-nano cellulose forms.
27522024	6	68	theme	microcrystalline	1258:1273	arg1	cellulose					1275:1283	microcrystalline cellulose	1258:1283	microcrystalline cellulose	1258:1283	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	4	69	theme	H2SO4	661:665	arg1	HNC					705:707	HNC	705:707	HNC	705:707	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	4	69	theme	H2SO4	661:665	arg1	cellulose					694:702	H2SO4 hydrolyzed nanocrystalline cellulose	661:702	H2SO4 hydrolyzed nanocrystalline cellulose (HNC)	661:708	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	7	70	theme	cel48	1338:1342	arg1	genes					1426:1430	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes	1308:1430	genes	1426:1430	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	6	71	theme	0.69 ± 0.04 wk-1for	1207:1225	arg1	0.58 ± 0.05 wk-1					1237:1252	0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1	1207:1252	1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium	1077:1304	HNC biodegraded at slightly faster rate than microcrystalline cellulose (1st order decay constants: 0.62 ± 0.08 wk-1 for HNC versus 0.39 ± 0.05 wk-1 for microcrystalline cellulose for the AD consortium; 0.69 ± 0.04 wk-1for HNCversus 0.58 ± 0.05 wk-1 for microcrystalline cellulose for the W consortium).
27522024	4	72	theme	cellulose	694:702	arg1	biodegradation					643:656	biodegradation	643:656	biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC)	643:708	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	8	73	dep	HNC	1643:1645	arg1	degradation					1681:1691	degradation	1681:1691	degradation	1681:1691	According to Illumina amplicon sequencing of 16S rRNA genes, the composition of the consortia underwent distinct shifts in concert with HNC versus microcrystalline cellulose degradation.
27522024	4	74	theme	wetland	883:889	arg1	inocula					895:901	anaerobic digester (AD) and wetland (W) inocula	855:901	inocula	895:901	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	3	75	theme	wastewater	577:586	arg1	treatment					588:596	wastewater treatment	577:596	wastewater treatment	577:596	Given its distinction in terms of size and chemical and physical properties, nanocellulose could potentially resist biodegradation and/or pose a xenobiotic influence on microbial communities during wastewater treatment or in receiving environments.
27522024	7	76	theme	glycoside	1345:1353	arg1	family					1370:1375	glycoside hydrolase gene family 48	1345:1378	glycoside hydrolase gene family 48	1345:1378	16S rRNA (total bacteria) and cel48 (glycoside hydrolase gene family 48, indicative of cellulose-degrading potential) genes were observed to be more enriched in the HNC condition for both consortia.
27522024	4	77	theme	nanocrystalline	678:692	arg1	HNC					705:707	HNC	705:707	HNC	705:707	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
27522024	4	77	theme	nanocrystalline	678:692	arg1	cellulose					694:702	H2SO4 hydrolyzed nanocrystalline cellulose	661:702	H2SO4 hydrolyzed nanocrystalline cellulose (HNC)	661:708	In this study, biodegradation of H2SO4 hydrolyzed nanocrystalline cellulose (HNC) was compared with that of microcrystalline cellulose using two distinct anaerobic cellulose-degrading microbial consortia initially sourced from anaerobic digester (AD) and wetland (W) inocula.
25372426	2	0	theme	analytical	576:585	arg1	technique					587:595	nondestructive analytical technique	561:595	nondestructive analytical technique for examining such NPs	561:618	Therefore, the availability of a multiscale (0.1-100 nm) and nondestructive analytical technique for examining such NPs is of great importance for nanotechnology.
25372426	1	1	theme	fundamental	379:389	arg1	knowledge					391:399	fundamental knowledge	379:399	fundamental knowledge for the precise design and efficient biological application of the NPs	379:470	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	8	2	theme	NMR	1592:1594	arg1	results					1596:1602	these NMR results	1586:1602	these NMR results	1586:1602	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	4	3	theme	developed	841:849	arg1	method					912:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	3	4	theme	multiscale	688:697	arg1	approach					715:722	a new multiscale solid-state NMR approach	682:722	a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs	682:820	Herein, we present a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs.
25372426	6	5	theme	coordinated	1232:1242	arg1	interaction					1244:1254	The interfacial coordinated interaction	1216:1254	The interfacial coordinated interaction between chitosan and phenylboronic acid	1216:1294	The interfacial coordinated interaction between chitosan and phenylboronic acid was revealed by one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR.
25372426	1	6	theme	efficient	428:436	arg1	application					449:459	efficient biological application	428:459	efficient biological application	428:459	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	1	7	theme	molecular-level	242:256	arg1	understanding					258:270	a molecular-level understanding	240:270	a molecular-level understanding	240:270	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	1	7	theme	molecular-level	242:256	arg1	critical					357:364	critical	357:364	critical	357:364	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	7	8	theme	C	1416:1416	arg1	MAS					1418:1420	dynamic-editing (13)C MAS	1396:1420	dynamic-editing (13)C MAS	1396:1420	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	2	9	theme	such	611:614	arg1	NPs					616:618	such NPs	611:618	such NPs	611:618	Therefore, the availability of a multiscale (0.1-100 nm) and nondestructive analytical technique for examining such NPs is of great importance for nanotechnology.
25372426	7	10	theme	swollen	1554:1560	arg1	states					1562:1567	the solid and swollen states	1540:1567	the solid and swollen states	1540:1567	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	4	11	theme	spinning	897:904	arg1	method					912:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	3	12	theme	NMR	711:713	arg1	approach					715:722	a new multiscale solid-state NMR approach	682:722	a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs	682:820	Herein, we present a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs.
25372426	6	13	theme	one-dimensional	1312:1326	arg1	MAS					1328:1330	one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR	1312:1384	MAS	1328:1330	The interfacial coordinated interaction between chitosan and phenylboronic acid was revealed by one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR.
25372426	8	14	theme	nanostructure	1627:1639	arg1	interaction					1654:1664	interfacial interaction	1642:1664	interfacial interaction	1642:1664	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	8	14	theme	nanostructure	1627:1639	arg1	model					1607:1611	a model	1605:1611	a model of the unique nanostructure	1605:1639	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	8	14	theme	nanostructure	1627:1639	arg1	dynamics					1684:1691	componential dynamics	1671:1691	componential dynamics of the NPs	1671:1702	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	7	15	theme	solid	1544:1548	arg1	states					1562:1567	the solid and swollen states	1540:1567	the solid and swollen states	1540:1567	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	4	16	theme	MAS	907:909	arg1	method					912:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	5	17	theme	internal	1141:1148	arg1	morphology					1150:1159	the internal morphology	1137:1159	the internal morphology	1137:1159	Then, using an improved (1)H spin-diffusion method with (13)C detection and theoretical simulations, the internal morphology and nanostructure size were quantitatively determined.
25372426	8	18	theme	interfacial	1642:1652	arg1	interaction					1654:1664	interfacial interaction	1642:1664	interfacial interaction	1642:1664	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	4	19	theme	C	855:855	arg1	method					912:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	1	20	theme	Chitosan-based	128:141	arg1	nanoparticles					143:155	Chitosan-based nanoparticles	128:155	Chitosan-based nanoparticles (NPs)	128:161	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	1	20	theme	Chitosan-based	128:141	arg1	NPs					158:160	NPs	158:160	NPs	158:160	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	4	21	theme	cross-polarization	866:883	arg1	method					912:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	3	22	theme	chitosan-poly	770:782	arg1	acid					812:815	N-3-acrylamidophenylboronic acid	784:815	N-3-acrylamidophenylboronic acid	784:815	Herein, we present a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs.
25372426	3	22	theme	chitosan-poly	770:782	arg1	NPs					818:820	chitosan-poly(N-3-acrylamidophenylboronic acid) NPs	770:820	chitosan-poly(N-3-acrylamidophenylboronic acid) NPs	770:820	Herein, we present a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs.
25372426	8	23	theme	componential	1671:1682	arg1	dynamics					1684:1691	componential dynamics	1671:1691	componential dynamics of the NPs	1671:1702	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	6	24	theme	MAS	1372:1374	arg1	NMR					1382:1384	one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR	1312:1384	NMR	1382:1384	The interfacial coordinated interaction between chitosan and phenylboronic acid was revealed by one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR.
25372426	5	25	theme	spin-diffusion	1065:1078	arg1	method					1080:1085	an improved (1)H spin-diffusion method	1048:1085	an improved (1)H spin-diffusion method	1048:1085	Then, using an improved (1)H spin-diffusion method with (13)C detection and theoretical simulations, the internal morphology and nanostructure size were quantitatively determined.
25372426	2	26	theme	multiscale	533:542	arg1	availability					515:526	the availability	511:526	the availability of a multiscale (0.1-100 nm) and nondestructive analytical technique for examining such NPs	511:618	Therefore, the availability of a multiscale (0.1-100 nm) and nondestructive analytical technique for examining such NPs is of great importance for nanotechnology.
25372426	0	27	theme	chitosan-based	68:81	arg1	nanoparticles					83:95	chitosan-based nanoparticles	68:95	chitosan-based nanoparticles	68:95	Probing the nanostructure, interfacial interaction, and dynamics of chitosan-based nanoparticles by multiscale solid-state NMR.
25372426	8	28	theme	NPs	1700:1702	arg1	interaction					1654:1664	interfacial interaction	1642:1664	interfacial interaction	1642:1664	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	8	28	theme	NPs	1700:1702	arg1	model					1607:1611	a model	1605:1611	a model of the unique nanostructure	1605:1639	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	8	28	theme	NPs	1700:1702	arg1	dynamics					1684:1691	componential dynamics	1671:1691	componential dynamics of the NPs	1671:1702	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	3	29	theme	N-3-acrylamidophenylboronic	784:810	arg1	acid					812:815	N-3-acrylamidophenylboronic acid	784:815	N-3-acrylamidophenylboronic acid	784:815	Herein, we present a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs.
25372426	3	29	theme	N-3-acrylamidophenylboronic	784:810	arg1	NPs					818:820	chitosan-poly(N-3-acrylamidophenylboronic acid) NPs	770:820	chitosan-poly(N-3-acrylamidophenylboronic acid) NPs	770:820	Herein, we present a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs.
25372426	1	30	theme	dynamics	338:345	arg1	understanding					258:270	a molecular-level understanding	240:270	a molecular-level understanding	240:270	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	1	30	theme	dynamics	338:345	arg1	critical					357:364	critical	357:364	critical	357:364	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	4	31	theme	fast	927:930	arg1	determination					945:957	fast quantitative determination	927:957	fast quantitative determination of the NPs' composition	927:981	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	0	32	theme	solid-state	111:121	arg1	NMR					123:125	multiscale solid-state NMR	100:125	multiscale solid-state NMR	100:125	Probing the nanostructure, interfacial interaction, and dynamics of chitosan-based nanoparticles by multiscale solid-state NMR.
25372426	1	33	theme	internal	279:286	arg1	morphology					288:297	the internal morphology	275:297	the internal morphology	275:297	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	4	34	from	changes	1015:1021	arg1	chitosan					1026:1033	chitosan	1026:1033	chitosan	1026:1033	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	4	35	theme	changes	1015:1021	arg1	determination					945:957	fast quantitative determination	927:957	fast quantitative determination of the NPs' composition	927:981	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	4	35	theme	changes	1015:1021	arg1	detection					987:995	detection	987:995	detection of conformational changes in chitosan	987:1033	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	8	36	dep	results	1596:1602	arg1	the					1573:1575	the	1573:1575	the	1573:1575	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	8	36	dep	results	1596:1602	arg1	basis					1577:1581	basis	1577:1581	basis	1577:1581	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	7	37	theme	H	1438:1438	arg1	wide-line					1440:1448	2D (13)C-(1)H wide-line	1426:1448	2D (13)C-(1)H wide-line	1426:1448	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	6	38	theme	B	1380:1380	arg1	NMR					1382:1384	one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR	1312:1384	NMR	1382:1384	The interfacial coordinated interaction between chitosan and phenylboronic acid was revealed by one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR.
25372426	1	39	theme	morphology	288:297	arg1	understanding					258:270	a molecular-level understanding	240:270	a molecular-level understanding	240:270	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	1	39	theme	morphology	288:297	arg1	critical					357:364	critical	357:364	critical	357:364	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	0	40	theme	interfacial	27:37	arg1	interaction					39:49	interfacial interaction	27:49	interfacial interaction	27:49	Probing the nanostructure, interfacial interaction, and dynamics of chitosan-based nanoparticles by multiscale solid-state NMR.
25372426	5	41	theme	13	1093:1094	arg1	C					1096:1096	C	1096:1096	C	1096:1096	Then, using an improved (1)H spin-diffusion method with (13)C detection and theoretical simulations, the internal morphology and nanostructure size were quantitatively determined.
25372426	5	42	theme	C	1096:1096	arg1	detection					1098:1106	(13)C detection	1092:1106	(13)C detection	1092:1106	Then, using an improved (1)H spin-diffusion method with (13)C detection and theoretical simulations, the internal morphology and nanostructure size were quantitatively determined.
25372426	7	43	theme	C-	1433:1434	arg1	wide-line					1440:1448	2D (13)C-(1)H wide-line	1426:1448	2D (13)C-(1)H wide-line	1426:1448	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	1	44	theme	great	483:487	arg1	challenge					489:497	a great challenge	481:497	a great challenge	481:497	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	5	45	theme	nanostructure	1165:1177	arg1	size					1179:1182	nanostructure size	1165:1182	nanostructure size	1165:1182	Then, using an improved (1)H spin-diffusion method with (13)C detection and theoretical simulations, the internal morphology and nanostructure size were quantitatively determined.
25372426	7	46	from	dynamics	1517:1524	arg1	states					1562:1567	the solid and swollen states	1540:1567	the solid and swollen states	1540:1567	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	0	47	dep	nanostructure	12:24	arg1	the					8:10	the	8:10	the	8:10	Probing the nanostructure, interfacial interaction, and dynamics of chitosan-based nanoparticles by multiscale solid-state NMR.
25372426	4	48	from	determination	945:957	arg1	chitosan					1026:1033	chitosan	1026:1033	chitosan	1026:1033	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	7	49	theme	2D	1426:1427	arg1	wide-line					1440:1448	2D (13)C-(1)H wide-line	1426:1448	2D (13)C-(1)H wide-line	1426:1448	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	6	50	theme	triple-quantum	1357:1370	arg1	NMR					1382:1384	one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR	1312:1384	NMR	1382:1384	The interfacial coordinated interaction between chitosan and phenylboronic acid was revealed by one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR.
25372426	7	51	theme	MAS	1418:1420	arg1	experiments					1461:1471	dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments	1396:1471	dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments	1396:1471	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	2	52	theme	nondestructive	561:574	arg1	technique					587:595	nondestructive analytical technique	561:595	nondestructive analytical technique for examining such NPs	561:618	Therefore, the availability of a multiscale (0.1-100 nm) and nondestructive analytical technique for examining such NPs is of great importance for nanotechnology.
25372426	7	53	theme	dynamic-editing	1396:1410	arg1	MAS					1418:1420	dynamic-editing (13)C MAS	1396:1420	dynamic-editing (13)C MAS	1396:1420	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	3	54	theme	solid-state	699:709	arg1	approach					715:722	a new multiscale solid-state NMR approach	682:722	a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs	682:820	Herein, we present a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs.
25372426	6	55	theme	interfacial	1220:1230	arg1	interaction					1244:1254	The interfacial coordinated interaction	1216:1254	The interfacial coordinated interaction between chitosan and phenylboronic acid	1216:1294	The interfacial coordinated interaction between chitosan and phenylboronic acid was revealed by one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR.
25372426	1	56	theme	biological	438:447	arg1	application					449:459	efficient biological application	428:459	efficient biological application	428:459	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	1	57	theme	nanostructure	303:315	arg1	size					317:320	nanostructure size	303:320	nanostructure size	303:320	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	3	58	theme	new	684:686	arg1	approach					715:722	a new multiscale solid-state NMR approach	682:722	a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs	682:820	Herein, we present a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs.
25372426	1	59	theme	drug	182:185	arg1	delivery					196:203	drug and gene delivery	182:203	delivery	196:203	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	2	60	theme	technique	587:595	arg1	availability					515:526	the availability	511:526	the availability of a multiscale (0.1-100 nm) and nondestructive analytical technique for examining such NPs	511:618	Therefore, the availability of a multiscale (0.1-100 nm) and nondestructive analytical technique for examining such NPs is of great importance for nanotechnology.
25372426	1	61	theme	NPs	468:470	arg1	design					417:422	the precise design	405:422	the precise design	405:422	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	1	61	theme	NPs	468:470	arg1	application					449:459	efficient biological application	428:459	efficient biological application	428:459	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	1	62	theme	gene	191:194	arg1	delivery					196:203	drug and gene delivery	182:203	delivery	196:203	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	4	63	theme	magic-angle	885:895	arg1	method					912:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	8	64	theme	unique	1620:1625	arg1	nanostructure					1627:1639	the unique nanostructure	1616:1639	the unique nanostructure	1616:1639	On the basis of these NMR results, a model of the unique nanostructure, interfacial interaction, and componential dynamics of the NPs was proposed.
25372426	7	65	theme	NPs	1533:1535	arg1	dynamics					1517:1524	the componential dynamics	1500:1524	the componential dynamics of the NPs in the solid and swollen states	1500:1567	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	6	66	theme	2D	1353:1354	arg1	NMR					1382:1384	one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR	1312:1384	NMR	1382:1384	The interfacial coordinated interaction between chitosan and phenylboronic acid was revealed by one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR.
25372426	5	67	theme	improved	1051:1058	arg1	method					1080:1085	an improved (1)H spin-diffusion method	1048:1085	an improved (1)H spin-diffusion method	1048:1085	Then, using an improved (1)H spin-diffusion method with (13)C detection and theoretical simulations, the internal morphology and nanostructure size were quantitatively determined.
25372426	6	68	theme	phenylboronic	1277:1289	arg1	acid					1291:1294	phenylboronic acid	1277:1294	phenylboronic acid	1277:1294	The interfacial coordinated interaction between chitosan and phenylboronic acid was revealed by one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR.
25372426	7	69	theme	componential	1504:1515	arg1	dynamics					1517:1524	the componential dynamics	1500:1524	the componential dynamics of the NPs in the solid and swollen states	1500:1567	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	4	70	theme	multiple	857:864	arg1	method					912:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method	830:917	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	1	71	theme	precise	409:415	arg1	design					417:422	the precise design	405:422	the precise design	405:422	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	4	72	theme	quantitative	932:943	arg1	determination					945:957	fast quantitative determination	927:957	fast quantitative determination of the NPs' composition	927:981	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	1	73	used	used	174:177	arg2	NPs					158:160	NPs	158:160	NPs	158:160	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	1	73	used	used	174:177	arg2	nanoparticles					143:155	Chitosan-based nanoparticles	128:155	Chitosan-based nanoparticles (NPs)	128:161	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	0	74	theme	nanoparticles	83:95	arg1	nanostructure					12:24	nanostructure	12:24	nanostructure	12:24	Probing the nanostructure, interfacial interaction, and dynamics of chitosan-based nanoparticles by multiscale solid-state NMR.
25372426	0	74	theme	nanoparticles	83:95	arg1	interaction					39:49	interfacial interaction	27:49	interfacial interaction	27:49	Probing the nanostructure, interfacial interaction, and dynamics of chitosan-based nanoparticles by multiscale solid-state NMR.
25372426	0	74	theme	nanoparticles	83:95	arg1	dynamics					56:63	dynamics	56:63	dynamics	56:63	Probing the nanostructure, interfacial interaction, and dynamics of chitosan-based nanoparticles by multiscale solid-state NMR.
25372426	1	75	theme	interface	323:331	arg1	understanding					258:270	a molecular-level understanding	240:270	a molecular-level understanding	240:270	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	1	75	theme	interface	323:331	arg1	critical					357:364	critical	357:364	critical	357:364	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	4	76	from	chitosan	1026:1033	arg1	determination					945:957	fast quantitative determination	927:957	fast quantitative determination of the NPs' composition	927:981	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	4	76	from	chitosan	1026:1033	arg1	detection					987:995	detection	987:995	detection of conformational changes in chitosan	987:1033	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	2	77	theme	great	626:630	arg1	importance					632:641	great importance	626:641	great importance	626:641	Therefore, the availability of a multiscale (0.1-100 nm) and nondestructive analytical technique for examining such NPs is of great importance for nanotechnology.
25372426	3	78	theme	NPs	818:820	arg1	investigation					753:765	the investigation	749:765	the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs	749:820	Herein, we present a new multiscale solid-state NMR approach to achieve this goal for the investigation of chitosan-poly(N-3-acrylamidophenylboronic acid) NPs.
25372426	0	79	theme	multiscale	100:109	arg1	NMR					123:125	multiscale solid-state NMR	100:125	multiscale solid-state NMR	100:125	Probing the nanostructure, interfacial interaction, and dynamics of chitosan-based nanoparticles by multiscale solid-state NMR.
25372426	7	80	theme	separation	1450:1459	arg1	experiments					1461:1471	dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments	1396:1471	dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments	1396:1471	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	6	81	theme	two-dimensional	1336:1350	arg1	NMR					1382:1384	one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR	1312:1384	NMR	1382:1384	The interfacial coordinated interaction between chitosan and phenylboronic acid was revealed by one-dimensional MAS and two-dimensional (2D) triple-quantum MAS (11)B NMR.
25372426	5	82	theme	H	1063:1063	arg1	method					1080:1085	an improved (1)H spin-diffusion method	1048:1085	an improved (1)H spin-diffusion method	1048:1085	Then, using an improved (1)H spin-diffusion method with (13)C detection and theoretical simulations, the internal morphology and nanostructure size were quantitatively determined.
25372426	5	83	theme	theoretical	1112:1122	arg1	simulations					1124:1134	theoretical simulations	1112:1134	theoretical simulations	1112:1134	Then, using an improved (1)H spin-diffusion method with (13)C detection and theoretical simulations, the internal morphology and nanostructure size were quantitatively determined.
25372426	4	84	theme	conformational	1000:1013	arg1	changes					1015:1021	conformational changes	1000:1021	conformational changes in chitosan	1000:1033	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	4	85	from	detection	987:995	arg1	chitosan					1026:1033	chitosan	1026:1033	chitosan	1026:1033	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	7	86	theme	wide-line	1440:1448	arg1	experiments					1461:1471	dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments	1396:1471	dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments	1396:1471	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
25372426	1	87	theme	medical	219:225	arg1	imaging					227:233	medical imaging	219:233	medical imaging	219:233	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	4	88	theme	composition	971:981	arg1	determination					945:957	fast quantitative determination	927:957	fast quantitative determination of the NPs' composition	927:981	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	4	88	theme	composition	971:981	arg1	detection					987:995	detection	987:995	detection of conformational changes in chitosan	987:1033	First, a recently developed (13)C multiple cross-polarization magic-angle spinning (MAS) method enabled fast quantitative determination of the NPs' composition and detection of conformational changes in chitosan.
25372426	1	89	theme	size	317:320	arg1	understanding					258:270	a molecular-level understanding	240:270	a molecular-level understanding	240:270	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	1	89	theme	size	317:320	arg1	critical					357:364	critical	357:364	critical	357:364	Chitosan-based nanoparticles (NPs) are widely used in drug and gene delivery, therapy, and medical imaging, but a molecular-level understanding of the internal morphology and nanostructure size, interface, and dynamics, which is critical for building fundamental knowledge for the precise design and efficient biological application of the NPs, remains a great challenge.
25372426	7	90	dep	wide-line	1440:1448	arg1	1					1436:1436	1	1436:1436	1	1436:1436	Finally, dynamic-editing (13)C MAS and 2D (13)C-(1)H wide-line separation experiments provided details regarding the componential dynamics of the NPs in the solid and swollen states.
24607421	3	0	theme	human	465:469	arg1	MSCs					471:474	human MSCs	465:474	human MSCs cultured within hydrogel matrices with tailored stiffness and composition	465:548	Here, the behaviour of human MSCs cultured within hydrogel matrices with tailored stiffness and composition was evaluated.
24607421	9	1	theme	matrices	1521:1528	arg1	design					1490:1495	the design	1486:1495	the design of cell-instructive 3-D matrices	1486:1528	These data provide further insights into the mechanisms by which MSCs sense their microenvironment to organize into tissues, and provide new clues to the design of cell-instructive 3-D matrices.
24607421	0	2	theme	alginate	91:98	arg1	hydrogels					100:108	soft alginate hydrogels	86:108	soft alginate hydrogels	86:108	Matrix-driven formation of mesenchymal stem cell-extracellular matrix microtissues on soft alginate hydrogels.
24607421	3	3	theme	MSCs	471:474	arg1	behaviour					452:460	the behaviour	448:460	the behaviour of human MSCs cultured within hydrogel matrices with tailored stiffness and composition	448:548	Here, the behaviour of human MSCs cultured within hydrogel matrices with tailored stiffness and composition was evaluated.
24607421	9	4	theme	new	1473:1475	arg1	clues					1477:1481	new clues	1473:1481	new clues to the design of cell-instructive 3-D matrices	1473:1528	These data provide further insights into the mechanisms by which MSCs sense their microenvironment to organize into tissues, and provide new clues to the design of cell-instructive 3-D matrices.
24607421	7	5	theme	cell	1043:1046	arg1	proliferation					1048:1060	cell proliferation	1043:1060	cell proliferation	1043:1060	Moreover, compliant matrices facilitated cell proliferation and provided an environment conducive for MSC osteogenic differentiation, even without RGD.
24607421	8	6	theme	fate	1330:1333	arg1	determinant					1310:1320	a more critical determinant	1294:1320	a more critical determinant of cell fate	1294:1333	Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate.
24607421	9	7	theme	3-D	1517:1519	arg1	matrices					1521:1528	cell-instructive 3-D matrices	1500:1528	cell-instructive 3-D matrices	1500:1528	These data provide further insights into the mechanisms by which MSCs sense their microenvironment to organize into tissues, and provide new clues to the design of cell-instructive 3-D matrices.
24607421	3	8	theme	tailored	515:522	arg1	stiffness					524:532	tailored stiffness	515:532	tailored stiffness	515:532	Here, the behaviour of human MSCs cultured within hydrogel matrices with tailored stiffness and composition was evaluated.
24607421	4	9	theme	MSC	565:567	arg1	aggregation					569:579	MSC aggregation	565:579	MSC aggregation	565:579	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	5	10	theme	cell-cell	834:842	arg1	contacts					844:851	cell-cell contacts	834:851	cell-cell contacts	834:851	Fibronectin assembly stabilized cell-cell contacts within aggregates, even in non-adhesive matrices.
24607421	2	11	dep	three-dimensional	409:425	arg1	3-D					428:430	3-D	428:430	3-D	428:430	The effect of such cues, and especially their interplay, is still not fully understood, particularly in three-dimensional (3-D) systems.
24607421	4	12	theme	compliant	603:611	arg1	matrices					613:620	more compliant matrices	598:620	more compliant matrices (G'≤ 120 Pa)	598:633	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	4	12	theme	compliant	603:611	arg1	Pa					631:632	G'≤ 120 Pa	623:632	G'≤ 120 Pa	623:632	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	3	13	theme	hydrogel	492:499	arg1	matrices					501:508	hydrogel matrices	492:508	hydrogel matrices with tailored stiffness and composition	492:548	Here, the behaviour of human MSCs cultured within hydrogel matrices with tailored stiffness and composition was evaluated.
24607421	4	14	theme	≤	625:625	arg1	matrices					613:620	more compliant matrices	598:620	more compliant matrices (G'≤ 120 Pa)	598:633	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	4	14	theme	≤	625:625	arg1	Pa					631:632	G'≤ 120 Pa	623:632	G'≤ 120 Pa	623:632	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	7	15	theme	conducive	1090:1098	arg1	environment					1078:1088	an environment	1075:1088	an environment conducive for MSC osteogenic differentiation	1075:1133	Moreover, compliant matrices facilitated cell proliferation and provided an environment conducive for MSC osteogenic differentiation, even without RGD.
24607421	8	16	theme	critical	1301:1308	arg1	determinant					1310:1320	a more critical determinant	1294:1320	a more critical determinant of cell fate	1294:1333	Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate.
24607421	8	17	theme	original	1181:1188	arg1	matrix					1190:1195	the original matrix	1177:1195	the original matrix	1177:1195	Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate.
24607421	8	18	theme	cell	1325:1328	arg1	fate					1330:1333	cell fate	1325:1333	cell fate	1325:1333	Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate.
24607421	0	19	theme	Matrix-driven	0:12	arg1	formation					14:22	Matrix-driven formation	0:22	Matrix-driven formation of mesenchymal stem cell-extracellular matrix	0:68	Matrix-driven formation of mesenchymal stem cell-extracellular matrix microtissues on soft alginate hydrogels.
24607421	7	20	theme	compliant	1012:1020	arg1	matrices					1022:1029	compliant matrices	1012:1029	compliant matrices	1012:1029	Moreover, compliant matrices facilitated cell proliferation and provided an environment conducive for MSC osteogenic differentiation, even without RGD.
24607421	1	21	theme	Mesenchymal	111:121	arg1	MSCs					135:138	MSCs	135:138	MSCs	135:138	Mesenchymal stem cells (MSCs) can be made to rearrange into microtissues in response to specific matrix cues, a process that depends on a balance between cell-matrix and cell-cell interactions.
24607421	1	21	theme	Mesenchymal	111:121	arg1	cells					128:132	Mesenchymal stem cells	111:132	Mesenchymal stem cells (MSCs)	111:139	Mesenchymal stem cells (MSCs) can be made to rearrange into microtissues in response to specific matrix cues, a process that depends on a balance between cell-matrix and cell-cell interactions.
24607421	0	22	theme	mesenchymal	27:37	arg1	matrix					63:68	mesenchymal stem cell-extracellular matrix	27:68	mesenchymal stem cell-extracellular matrix	27:68	Matrix-driven formation of mesenchymal stem cell-extracellular matrix microtissues on soft alginate hydrogels.
24607421	1	23	theme	stem	123:126	arg1	MSCs					135:138	MSCs	135:138	MSCs	135:138	Mesenchymal stem cells (MSCs) can be made to rearrange into microtissues in response to specific matrix cues, a process that depends on a balance between cell-matrix and cell-cell interactions.
24607421	1	23	theme	stem	123:126	arg1	cells					128:132	Mesenchymal stem cells	111:132	Mesenchymal stem cells (MSCs)	111:139	Mesenchymal stem cells (MSCs) can be made to rearrange into microtissues in response to specific matrix cues, a process that depends on a balance between cell-matrix and cell-cell interactions.
24607421	4	24	dep	RGD	777:779	arg1	μM					797:798	0, 100 and 200 μM	782:798	RGD; 0, 100 and 200 μM	777:798	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	7	25	theme	osteogenic	1108:1117	arg1	differentiation					1119:1133	MSC osteogenic differentiation	1104:1133	MSC osteogenic differentiation	1104:1133	Moreover, compliant matrices facilitated cell proliferation and provided an environment conducive for MSC osteogenic differentiation, even without RGD.
24607421	5	26	theme	non-adhesive	880:891	arg1	matrices					893:900	non-adhesive matrices	880:900	non-adhesive matrices	880:900	Fibronectin assembly stabilized cell-cell contacts within aggregates, even in non-adhesive matrices.
24607421	1	27	theme	specific	199:206	arg1	process					223:229	a process	221:229	a process that depends on a balance between cell-matrix and cell-cell interactions	221:302	Mesenchymal stem cells (MSCs) can be made to rearrange into microtissues in response to specific matrix cues, a process that depends on a balance between cell-matrix and cell-cell interactions.
24607421	1	27	theme	specific	199:206	arg1	cues					215:218	specific matrix cues	199:218	specific matrix cues	199:218	Mesenchymal stem cells (MSCs) can be made to rearrange into microtissues in response to specific matrix cues, a process that depends on a balance between cell-matrix and cell-cell interactions.
24607421	0	28	theme	cell-extracellular	44:61	arg1	matrix					63:68	mesenchymal stem cell-extracellular matrix	27:68	mesenchymal stem cell-extracellular matrix	27:68	Matrix-driven formation of mesenchymal stem cell-extracellular matrix microtissues on soft alginate hydrogels.
24607421	1	29	theme	cell-cell	281:289	arg1	interactions					291:302	cell-cell interactions	281:302	cell-cell interactions	281:302	Mesenchymal stem cells (MSCs) can be made to rearrange into microtissues in response to specific matrix cues, a process that depends on a balance between cell-matrix and cell-cell interactions.
24607421	4	30	dep	ligands	768:774	arg1	RGD					777:779	RGD	777:779	RGD; 0, 100 and 200 μM	777:798	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	4	31	theme	ligands	768:774	arg1	absence					699:705	the absence	695:705	the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM)	695:799	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	1	32	theme	matrix	208:213	arg1	process					223:229	a process	221:229	a process that depends on a balance between cell-matrix and cell-cell interactions	221:302	Mesenchymal stem cells (MSCs) can be made to rearrange into microtissues in response to specific matrix cues, a process that depends on a balance between cell-matrix and cell-cell interactions.
24607421	1	32	theme	matrix	208:213	arg1	cues					215:218	specific matrix cues	199:218	specific matrix cues	199:218	Mesenchymal stem cells (MSCs) can be made to rearrange into microtissues in response to specific matrix cues, a process that depends on a balance between cell-matrix and cell-cell interactions.
24607421	0	33	theme	stem	39:42	arg1	matrix					63:68	mesenchymal stem cell-extracellular matrix	27:68	mesenchymal stem cell-extracellular matrix	27:68	Matrix-driven formation of mesenchymal stem cell-extracellular matrix microtissues on soft alginate hydrogels.
24607421	3	34	with	matrices	501:508	arg1	composition					538:548	composition	538:548	composition	538:548	Here, the behaviour of human MSCs cultured within hydrogel matrices with tailored stiffness and composition was evaluated.
24607421	3	34	with	matrices	501:508	arg1	stiffness					524:532	tailored stiffness	515:532	tailored stiffness	515:532	Here, the behaviour of human MSCs cultured within hydrogel matrices with tailored stiffness and composition was evaluated.
24607421	2	35	theme	three-dimensional	409:425	arg1	systems					433:439	three-dimensional (3-D) systems	409:439	three-dimensional (3-D) systems	409:439	The effect of such cues, and especially their interplay, is still not fully understood, particularly in three-dimensional (3-D) systems.
24607421	8	36	theme	time	1222:1225	arg1	progressed					1227:1236	time progressed	1222:1236	time progressed	1222:1236	Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate.
24607421	8	37	theme	Cell	1154:1157	arg1	interactions					1159:1170	Cell interactions	1154:1170	Cell interactions with the original matrix	1154:1195	Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate.
24607421	4	38	theme	stiffer	653:659	arg1	ones					661:664	stiffer ones	653:664	stiffer ones	653:664	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	0	39	theme	matrix	63:68	arg1	formation					14:22	Matrix-driven formation	0:22	Matrix-driven formation of mesenchymal stem cell-extracellular matrix	0:68	Matrix-driven formation of mesenchymal stem cell-extracellular matrix microtissues on soft alginate hydrogels.
24607421	2	40	theme	cues	324:327	arg1	interplay					351:359	their interplay	345:359	their interplay	345:359	The effect of such cues, and especially their interplay, is still not fully understood, particularly in three-dimensional (3-D) systems.
24607421	2	40	theme	cues	324:327	arg1	effect					309:314	The effect	305:314	The effect of such cues	305:327	The effect of such cues, and especially their interplay, is still not fully understood, particularly in three-dimensional (3-D) systems.
24607421	7	41	theme	MSC	1104:1106	arg1	differentiation					1119:1133	MSC osteogenic differentiation	1104:1133	MSC osteogenic differentiation	1104:1133	Moreover, compliant matrices facilitated cell proliferation and provided an environment conducive for MSC osteogenic differentiation, even without RGD.
24607421	4	42	theme	matrix-bound	710:721	arg1	ligands					768:774	matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands	710:774	matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM)	710:799	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	4	43	theme	cell-adhesion	754:766	arg1	ligands					768:774	matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands	710:774	matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM)	710:799	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	2	44	theme	such	319:322	arg1	cues					324:327	such cues	319:327	such cues	319:327	The effect of such cues, and especially their interplay, is still not fully understood, particularly in three-dimensional (3-D) systems.
24607421	6	45	theme	artificial	957:966	arg1	matrix					968:973	the artificial matrix	953:973	the artificial matrix	953:973	However, MSCs were able to substantially contract the artificial matrix only when RGD was present.
24607421	9	46	theme	cell-instructive	1500:1515	arg1	matrices					1521:1528	cell-instructive 3-D matrices	1500:1528	cell-instructive 3-D matrices	1500:1528	These data provide further insights into the mechanisms by which MSCs sense their microenvironment to organize into tissues, and provide new clues to the design of cell-instructive 3-D matrices.
24607421	8	47	theme	novo-produced	1252:1264	arg1	matrix					1280:1285	the de novo-produced extracellular matrix	1245:1285	the de novo-produced extracellular matrix	1245:1285	Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate.
24607421	8	48	theme	de	1249:1250	arg1	matrix					1280:1285	the de novo-produced extracellular matrix	1245:1285	the de novo-produced extracellular matrix	1245:1285	Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate.
24607421	0	49	theme	soft	86:89	arg1	hydrogels					100:108	soft alginate hydrogels	86:108	soft alginate hydrogels	86:108	Matrix-driven formation of mesenchymal stem cell-extracellular matrix microtissues on soft alginate hydrogels.
24607421	4	50	theme	acid	749:752	arg1	ligands					768:774	matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands	710:774	matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM)	710:799	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	8	51	theme	extracellular	1266:1278	arg1	matrix					1280:1285	the de novo-produced extracellular matrix	1245:1285	the de novo-produced extracellular matrix	1245:1285	Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate.
24607421	4	52	theme	arginine-glycine-aspartic	723:747	arg1	ligands					768:774	matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands	710:774	matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM)	710:799	MSC aggregation occurred only in more compliant matrices (G'≤ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine-glycine-aspartic acid cell-adhesion ligands (RGD; 0, 100 and 200 μM).
24607421	8	53	with	interactions	1159:1170	arg1	matrix					1190:1195	the original matrix	1177:1195	the original matrix	1177:1195	Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate.
24607421	5	54	theme	Fibronectin	802:812	arg1	assembly					814:821	Fibronectin assembly	802:821	Fibronectin assembly	802:821	Fibronectin assembly stabilized cell-cell contacts within aggregates, even in non-adhesive matrices.
24607421	9	55	theme	further	1355:1361	arg1	insights					1363:1370	further insights	1355:1370	further insights into the mechanisms by which MSCs sense their microenvironment to organize into tissues	1355:1458	These data provide further insights into the mechanisms by which MSCs sense their microenvironment to organize into tissues, and provide new clues to the design of cell-instructive 3-D matrices.
25281067	7	0	theme	good	1358:1361	arg1	results					1363:1369	good results	1358:1369	good results	1358:1369	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	5	1	theme	different	863:871	arg1	manipulations					904:916	two different post-acquisition spectroscopic manipulations	859:916	two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration)	859:1008	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	5	2	theme	reference	726:734	arg1	data					736:739	different reference data	716:739	different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values)	716:852	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	1	3	theme	ATR-FTIR	247:254	arg1	analysis					256:263	ATR-FTIR analysis	247:263	ATR-FTIR analysis	247:263	This paper reports on the assessment of lignin and holocellulose by means of ATR-FTIR analysis and multivariate PLS regression.
25281067	2	4	theme	different	351:359	arg1	excavations					361:371	different excavations	351:371	different excavations where wood had been preserved in waterlogged conditions	351:427	The analysis was conducted on 59 samples coming from different excavations where wood had been preserved in waterlogged conditions.
25281067	7	5	theme	sample	1490:1495	arg1	extraction					1476:1485	the preliminary extraction	1460:1485	the preliminary extraction of sample	1460:1495	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	0	6	theme	chemical	96:103	arg1	composition					105:115	the chemical composition	92:115	the chemical composition of	92:118	Quantitative evaluation by attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of decayed wood preserved in waterlogged conditions.
25281067	7	7	theme	high	1277:1280	arg1	content					1286:1292	high ash content	1277:1292	high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction)	1277:1447	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	7	8	dep	content	1286:1292	arg1	had					1320:1322	had	1320:1322	had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction	1320:1446	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	5	9	theme	procedures	950:959	arg1	terms					927:931	terms	927:931	terms of normalisation procedures and of spectral ranges used for the calibration	927:1007	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	5	10	dep	samples	742:748	arg1	extracted					750:758	extracted	750:758	extracted	750:758	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	5	10	dep	samples	742:748	arg1	not-extracted					764:776	not-extracted	764:776	not-extracted	764:776	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	5	10	dep	samples	742:748	arg1	bases					805:809	and ash-rich and ash-free bases	779:809	and ash-rich and ash-free bases of calculation for the calibration values	779:851	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	7	11	theme	preliminary	1464:1474	arg1	extraction					1476:1485	the preliminary extraction	1460:1485	the preliminary extraction of sample	1460:1495	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	6	12	theme	data	1154:1157	arg1	set					1159:1161	data set	1154:1161	data set (softwood or hardwood)	1154:1184	Results showed that the best models were different depending on which considered component (lignin or holocellulose) was measured and to which data set (softwood or hardwood) the samples belonged.
25281067	8	13	theme	external	1618:1625	arg1	set					1627:1629	an external set	1615:1629	an external set of samples	1615:1640	Furthermore, the stability of best models is also demonstrated and a procedure of external validation carried out on an external set of samples confirmed the general validity of the identified models.
25281067	8	13	theme	external	1618:1625	arg1	samples					1634:1640	samples	1634:1640	samples	1634:1640	Furthermore, the stability of best models is also demonstrated and a procedure of external validation carried out on an external set of samples confirmed the general validity of the identified models.
25281067	1	14	theme	multivariate	269:280	arg1	regression					286:295	multivariate PLS regression	269:295	multivariate PLS regression	269:295	This paper reports on the assessment of lignin and holocellulose by means of ATR-FTIR analysis and multivariate PLS regression.
25281067	5	15	theme	different	716:724	arg1	data					736:739	different reference data	716:739	different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values)	716:852	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	7	16	theme	predictive	1229:1238	arg1	ability					1240:1246	the predictive ability	1225:1246	the predictive ability of the models	1225:1260	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	5	17	theme	calculation	814:824	arg1	bases					805:809	and ash-rich and ash-free bases	779:809	and ash-rich and ash-free bases of calculation for the calibration values	779:851	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	6	18	dep	set	1159:1161	arg1	hardwood					1176:1183	hardwood	1176:1183	hardwood	1176:1183	Results showed that the best models were different depending on which considered component (lignin or holocellulose) was measured and to which data set (softwood or hardwood) the samples belonged.
25281067	6	18	dep	set	1159:1161	arg1	softwood					1164:1171	softwood	1164:1171	softwood	1164:1171	Results showed that the best models were different depending on which considered component (lignin or holocellulose) was measured and to which data set (softwood or hardwood) the samples belonged.
25281067	4	19	theme	times	646:650	arg1	states					585:590	states	585:590	states	585:590	, states of preservation, waterlogged environments, and burial times are presented.
25281067	3	20	theme	results	441:447	arg1	range					432:436	A range	430:436	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)	430:582	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	0	21	theme	composition	105:115	arg1	spectroscopy					76:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy	27:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of	27:118	Quantitative evaluation by attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of decayed wood preserved in waterlogged conditions.
25281067	5	22	theme	post-acquisition	873:888	arg1	manipulations					904:916	two different post-acquisition spectroscopic manipulations	859:916	two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration)	859:1008	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	3	23	theme	Picea	530:534	arg1	abies					536:540	Picea abies	530:540	Picea abies	530:540	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	3	23	theme	Picea	530:534	arg1	sp.p.					484:488	Alnus sp.p.	478:488	Alnus sp.p.	478:488	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	7	24	theme	ash	1282:1284	arg1	content					1286:1292	high ash content	1277:1292	high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction)	1277:1447	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	5	25	theme	ash-rich	783:790	arg1	bases					805:809	and ash-rich and ash-free bases	779:809	and ash-rich and ash-free bases of calculation for the calibration values	779:851	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	4	26	theme	environments	621:632	arg1	states					585:590	states	585:590	states	585:590	, states of preservation, waterlogged environments, and burial times are presented.
25281067	0	27	theme	Quantitative	0:11	arg1	evaluation					13:22	Quantitative evaluation	0:22	Quantitative evaluation by attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of	0:118	Quantitative evaluation by attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of decayed wood preserved in waterlogged conditions.
25281067	1	28	theme	PLS	282:284	arg1	regression					286:295	multivariate PLS regression	269:295	multivariate PLS regression	269:295	This paper reports on the assessment of lignin and holocellulose by means of ATR-FTIR analysis and multivariate PLS regression.
25281067	7	29	theme	inorganic	1429:1437	arg1	fraction					1439:1446	the inorganic fraction	1425:1446	the inorganic fraction	1425:1446	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	5	30	theme	calibration	834:844	arg1	values					846:851	the calibration values	830:851	the calibration values	830:851	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	3	31	theme	Quercus	556:562	arg1	sp.p.					564:568	Quercus sp.p.	556:568	Quercus sp.p.	556:568	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	3	31	theme	Quercus	556:562	arg1	sp.p.					484:488	Alnus sp.p.	478:488	Alnus sp.p.	478:488	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	4	32	theme	burial	639:644	arg1	times					646:650	burial times	639:650	burial times	639:650	, states of preservation, waterlogged environments, and burial times are presented.
25281067	0	33	theme	attenuated	27:36	arg1	spectroscopy					76:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy	27:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of	27:118	Quantitative evaluation by attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of decayed wood preserved in waterlogged conditions.
25281067	5	34	theme	calibration	669:679	arg1	model					681:685	A calibration model	667:685	A calibration model	667:685	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	5	35	from	manipulations	904:916	arg1	terms					927:931	terms	927:931	terms of normalisation procedures and of spectral ranges used for the calibration	927:1007	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	5	36	dep	terms	927:931	arg1	both					919:922	both	919:922	both	919:922	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	8	37	theme	samples	1634:1640	arg1	set					1627:1629	an external set	1615:1629	an external set of samples	1615:1640	Furthermore, the stability of best models is also demonstrated and a procedure of external validation carried out on an external set of samples confirmed the general validity of the identified models.
25281067	8	37	theme	samples	1634:1640	arg1	samples					1634:1640	samples	1634:1640	samples	1634:1640	Furthermore, the stability of best models is also demonstrated and a procedure of external validation carried out on an external set of samples confirmed the general validity of the identified models.
25281067	6	38	theme	best	1035:1038	arg1	different					1052:1060	different	1052:1060	different	1052:1060	Results showed that the best models were different depending on which considered component (lignin or holocellulose) was measured and to which data set (softwood or hardwood) the samples belonged.
25281067	6	38	theme	best	1035:1038	arg1	models					1040:1045	the best models	1031:1045	the best models	1031:1045	Results showed that the best models were different depending on which considered component (lignin or holocellulose) was measured and to which data set (softwood or hardwood) the samples belonged.
25281067	8	39	theme	models	1691:1696	arg1	validity					1664:1671	the general validity	1652:1671	the general validity of the identified models	1652:1696	Furthermore, the stability of best models is also demonstrated and a procedure of external validation carried out on an external set of samples confirmed the general validity of the identified models.
25281067	8	40	theme	identified	1680:1689	arg1	models					1691:1696	the identified models	1676:1696	the identified models	1676:1696	Furthermore, the stability of best models is also demonstrated and a procedure of external validation carried out on an external set of samples confirmed the general validity of the identified models.
25281067	5	41	theme	normalisation	936:948	arg1	procedures					950:959	normalisation procedures	936:959	normalisation procedures	936:959	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	8	42	theme	validation	1589:1598	arg1	procedure					1567:1575	a procedure	1565:1575	a procedure of external validation carried out on an external set of samples	1565:1640	Furthermore, the stability of best models is also demonstrated and a procedure of external validation carried out on an external set of samples confirmed the general validity of the identified models.
25281067	4	43	theme	preservation	595:606	arg1	states					585:590	states	585:590	states	585:590	, states of preservation, waterlogged environments, and burial times are presented.
25281067	0	44	theme	reflectance	44:54	arg1	spectroscopy					76:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy	27:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of	27:118	Quantitative evaluation by attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of decayed wood preserved in waterlogged conditions.
25281067	5	45	dep	data	736:739	arg1	samples					742:748	samples	742:748	samples extracted and not-extracted	742:776	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	1	46	theme	lignin	210:215	arg1	assessment					196:205	the assessment	192:205	the assessment of lignin and holocellulose	192:233	This paper reports on the assessment of lignin and holocellulose by means of ATR-FTIR analysis and multivariate PLS regression.
25281067	4	47	theme	waterlogged	609:619	arg1	environments					621:632	waterlogged environments	609:632	waterlogged environments	609:632	, states of preservation, waterlogged environments, and burial times are presented.
25281067	6	48	dep	measured	1132:1139	arg1	depending					1062:1070	depending	1062:1070	depending	1062:1070	Results showed that the best models were different depending on which considered component (lignin or holocellulose) was measured and to which data set (softwood or hardwood) the samples belonged.
25281067	3	49	theme	different	454:462	arg1	species					469:475	different wood species	454:475	different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)	454:582	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	0	50	theme	total	38:42	arg1	spectroscopy					76:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy	27:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of	27:118	Quantitative evaluation by attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of decayed wood preserved in waterlogged conditions.
25281067	5	51	theme	ash-free	796:803	arg1	bases					805:809	and ash-rich and ash-free bases	779:809	and ash-rich and ash-free bases of calculation for the calibration values	779:851	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	2	52	theme	waterlogged	406:416	arg1	conditions					418:427	waterlogged conditions	406:427	waterlogged conditions	406:427	The analysis was conducted on 59 samples coming from different excavations where wood had been preserved in waterlogged conditions.
25281067	6	53	dep	component	1092:1100	arg1	lignin					1103:1108	lignin	1103:1108	lignin	1103:1108	Results showed that the best models were different depending on which considered component (lignin or holocellulose) was measured and to which data set (softwood or hardwood) the samples belonged.
25281067	6	53	dep	component	1092:1100	arg1	holocellulose					1113:1125	holocellulose	1113:1125	holocellulose	1113:1125	Results showed that the best models were different depending on which considered component (lignin or holocellulose) was measured and to which data set (softwood or hardwood) the samples belonged.
25281067	5	54	theme	spectroscopic	890:902	arg1	manipulations					904:916	two different post-acquisition spectroscopic manipulations	859:916	two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration)	859:1008	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	0	55	theme	infrared	56:63	arg1	spectroscopy					76:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy	27:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of	27:118	Quantitative evaluation by attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of decayed wood preserved in waterlogged conditions.
25281067	0	56	theme	waterlogged	146:156	arg1	conditions					158:167	waterlogged conditions	146:167	waterlogged conditions	146:167	Quantitative evaluation by attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of decayed wood preserved in waterlogged conditions.
25281067	3	57	from	species	469:475	arg1	results					441:447	results	441:447	results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)	441:582	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	3	57	from	species	469:475	arg1	range					432:436	A range	430:436	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)	430:582	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	8	58	theme	general	1656:1662	arg1	validity					1664:1671	the general validity	1652:1671	the general validity of the identified models	1652:1696	Furthermore, the stability of best models is also demonstrated and a procedure of external validation carried out on an external set of samples confirmed the general validity of the identified models.
25281067	5	59	theme	ranges	977:982	arg1	terms					927:931	terms	927:931	terms of normalisation procedures and of spectral ranges used for the calibration	927:1007	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	7	60	theme	bands	1408:1412	arg1	overlapping					1393:1403	overlapping	1393:1403	overlapping	1393:1403	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	1	61	theme	holocellulose	221:233	arg1	assessment					196:205	the assessment	192:205	the assessment of lignin and holocellulose	192:233	This paper reports on the assessment of lignin and holocellulose by means of ATR-FTIR analysis and multivariate PLS regression.
25281067	5	62	theme	spectral	968:975	arg1	ranges					977:982	spectral ranges	968:982	spectral ranges used for the calibration	968:1007	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	5	63	from	data	736:739	arg1	terms					927:931	terms	927:931	terms of normalisation procedures and of spectral ranges used for the calibration	927:1007	A calibration model was selected after comparing different reference data (samples extracted and not-extracted, and ash-rich and ash-free bases of calculation for the calibration values), and two different post-acquisition spectroscopic manipulations (both in terms of normalisation procedures and of spectral ranges used for the calibration).
25281067	7	64	theme	contaminated	1299:1310	arg1	samples					1312:1318	too contaminated samples	1295:1318	too contaminated samples	1295:1318	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	3	65	from	range	432:436	arg1	species					469:475	different wood species	454:475	different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)	454:582	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	8	66	theme	external	1580:1587	arg1	validation					1589:1598	external validation	1580:1598	external validation carried out on an external set of samples	1580:1640	Furthermore, the stability of best models is also demonstrated and a procedure of external validation carried out on an external set of samples confirmed the general validity of the identified models.
25281067	3	67	theme	wood	464:467	arg1	species					469:475	different wood species	454:475	different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)	454:582	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	3	68	dep	species	469:475	arg1	sp.p.					577:581	Ulmus sp.p.	571:581	Ulmus sp.p.	571:581	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	3	68	dep	species	469:475	arg1	decidua					521:527	Larix decidua	515:527	Larix decidua	515:527	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	3	68	dep	species	469:475	arg1	sp.p.					549:553	Pinus sp.p.	543:553	Pinus sp.p.	543:553	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	3	68	dep	species	469:475	arg1	sp.p.					484:488	Alnus sp.p.	478:488	Alnus sp.p.	478:488	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	3	68	dep	species	469:475	arg1	abies					536:540	Picea abies	530:540	Picea abies	530:540	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	3	68	dep	species	469:475	arg1	sp.p.					564:568	Quercus sp.p.	556:568	Quercus sp.p.	556:568	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	3	68	dep	species	469:475	arg1	sempervirens					501:512	Cupressus sempervirens	491:512	Cupressus sempervirens	491:512	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	7	69	theme	models	1255:1260	arg1	ability					1240:1246	the predictive ability	1225:1246	the predictive ability of the models	1225:1260	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	3	70	theme	Larix	515:519	arg1	sp.p.					484:488	Alnus sp.p.	478:488	Alnus sp.p.	478:488	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	3	70	theme	Larix	515:519	arg1	decidua					521:527	Larix decidua	515:527	Larix decidua	515:527	A range of results from different wood species (Alnus sp.p., Cupressus sempervirens, Larix decidua, Picea abies, Pinus sp.p., Quercus sp.p., Ulmus sp.p.)
25281067	8	71	theme	models	1533:1538	arg1	stability					1515:1523	the stability	1511:1523	the stability of best models	1511:1538	Furthermore, the stability of best models is also demonstrated and a procedure of external validation carried out on an external set of samples confirmed the general validity of the identified models.
25281067	0	72	theme	ATR-FTIR	66:73	arg1	spectroscopy					76:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy	27:87	attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of	27:118	Quantitative evaluation by attenuated total reflectance infrared (ATR-FTIR) spectroscopy of the chemical composition of decayed wood preserved in waterlogged conditions.
25281067	7	73	theme	related	1414:1420	arg1	bands					1408:1412	bands	1408:1412	bands related to the inorganic fraction	1408:1446	It is shown that the predictive ability of the models is affected by high ash content (too contaminated samples had to be excluded in order to attain good results, because of excessive overlapping of bands related to the inorganic fraction) but not by the preliminary extraction of sample.
25281067	6	74	theme	considered	1081:1090	arg1	component					1092:1100	considered component	1081:1100	considered component (lignin or holocellulose)	1081:1126	Results showed that the best models were different depending on which considered component (lignin or holocellulose) was measured and to which data set (softwood or hardwood) the samples belonged.
25281067	8	75	theme	best	1528:1531	arg1	models					1533:1538	best models	1528:1538	best models	1528:1538	Furthermore, the stability of best models is also demonstrated and a procedure of external validation carried out on an external set of samples confirmed the general validity of the identified models.
28315770	2	0	dep	differentiated	200:213	arg1	"					222:222	"Janus"	216:222	"Janus"	216:222	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	9	1	theme	reduction	1608:1616	arg1	assays					1618:1623	MTT reduction assays	1604:1623	MTT reduction assays	1604:1623	Some of the CDplexes herein studied showed moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities, as determined by FACS and MTT reduction assays.
28315770	9	2	from	levels	1491:1496	arg1	cells					1523:1527	HeLa and MCF-7 cancer cells	1501:1527	HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities	1501:1575	Some of the CDplexes herein studied showed moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities, as determined by FACS and MTT reduction assays.
28315770	4	3	theme	plasmid	606:612	arg1	DNA					614:616	pEGFP-C3 plasmid DNA	597:616	pEGFP-C3 plasmid DNA	597:616	All of them compact pEGFP-C3 plasmid DNA and promote transfection in HeLa and MCF-7 cells, both in absence and in presence of human serum.
28315770	10	4	theme	association	1693:1703	arg1	processes					1705:1713	the paCD-pDNA-PC association processes	1676:1713	the paCD-pDNA-PC association processes	1676:1713	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	2	5	theme	pluridisciplinary	331:347	arg1	approach					349:356	a pluridisciplinary approach	329:356	a pluridisciplinary approach	329:356	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	4	6	theme	MCF-7	655:659	arg1	cells					661:665	HeLa and MCF-7 cells	646:665	HeLa and MCF-7 cells	646:665	All of them compact pEGFP-C3 plasmid DNA and promote transfection in HeLa and MCF-7 cells, both in absence and in presence of human serum.
28315770	7	7	theme	liquid	1065:1070	arg1	phases					1080:1085	multilamellar lyotropic liquid crystal phases	1041:1085	multilamellar lyotropic liquid crystal phases	1041:1085	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
28315770	10	8	theme	rational	1721:1728	arg1	base					1730:1733	a rational base	1719:1733	a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications	1719:1821	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	5	9	theme	electrochemical	720:734	arg1	characteristics					751:765	The electrochemical and structural characteristics	716:765	The electrochemical and structural characteristics of the paCD-pDNA complexes (CDplexes)	716:803	The electrochemical and structural characteristics of the paCD-pDNA complexes (CDplexes) have been studied by using zeta potential, DLS, SAXS, and cryo-TEM.
28315770	7	10	theme	CD-based	1144:1151	arg1	vectors					1153:1159	the CD-based vectors	1140:1159	the CD-based vectors with anionic pDNA sandwiched among them	1140:1199	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
28315770	7	11	theme	multilamellar	1041:1053	arg1	phases					1080:1085	multilamellar lyotropic liquid crystal phases	1041:1085	multilamellar lyotropic liquid crystal phases	1041:1085	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
28315770	2	12	theme	amphiphilic	238:248	arg1	paCDs					265:269	paCDs	265:269	paCDs	265:269	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	2	12	theme	amphiphilic	238:248	arg1	cyclodextrins					250:262	facially differentiated ("Janus") polycationic amphiphilic cyclodextrins	191:262	facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers	191:301	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	7	13	theme	self-assembling	1000:1014	arg1	pattern					1016:1022	a self-assembling pattern	998:1022	a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them	998:1199	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
28315770	2	14	theme	non-viral	275:283	arg1	nanocarriers					290:301	non-viral gene nanocarriers	275:301	non-viral gene nanocarriers	275:301	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	3	15	theme	representative	365:378	arg1	paCDs					386:390	Three representative Janus paCDs	359:390	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side	359:545	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	5	16	theme	paCD-pDNA	774:782	arg1	CDplexes					795:802	CDplexes	795:802	CDplexes	795:802	The electrochemical and structural characteristics of the paCD-pDNA complexes (CDplexes) have been studied by using zeta potential, DLS, SAXS, and cryo-TEM.
28315770	5	16	theme	paCD-pDNA	774:782	arg1	complexes					784:792	the paCD-pDNA complexes	770:792	the paCD-pDNA complexes (CDplexes)	770:803	The electrochemical and structural characteristics of the paCD-pDNA complexes (CDplexes) have been studied by using zeta potential, DLS, SAXS, and cryo-TEM.
28315770	10	17	theme	targeted	1769:1776	arg1	delivery					1783:1790	targeted gene delivery	1769:1790	targeted gene delivery	1769:1790	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	9	18	theme	moderate-to-high	1461:1476	arg1	levels					1491:1496	moderate-to-high transfection levels	1461:1496	moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities	1461:1575	Some of the CDplexes herein studied showed moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities, as determined by FACS and MTT reduction assays.
28315770	4	19	theme	human	703:707	arg1	serum					709:713	human serum	703:713	human serum	703:713	All of them compact pEGFP-C3 plasmid DNA and promote transfection in HeLa and MCF-7 cells, both in absence and in presence of human serum.
28315770	6	20	theme	nominal	966:972	arg1	ones					974:977	the nominal ones	962:977	the nominal ones	962:977	paCDs and pDNA, when assembled in CDplexes, render effective charges that are lower than the nominal ones.
28315770	5	21	theme	structural	740:749	arg1	characteristics					751:765	The electrochemical and structural characteristics	716:765	The electrochemical and structural characteristics of the paCD-pDNA complexes (CDplexes)	716:803	The electrochemical and structural characteristics of the paCD-pDNA complexes (CDplexes) have been studied by using zeta potential, DLS, SAXS, and cryo-TEM.
28315770	9	22	theme	HeLa	1501:1504	arg1	cells					1523:1527	HeLa and MCF-7 cancer cells	1501:1527	HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities	1501:1575	Some of the CDplexes herein studied showed moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities, as determined by FACS and MTT reduction assays.
28315770	8	23	theme	CD-based	1309:1316	arg1	vector					1318:1323	the cationic CD-based vector	1296:1323	the cationic CD-based vector	1296:1323	When exposed to human serum, either in the absence or in the presence of pDNA, the surface of the cationic CD-based vector becomes coated by a protein corona (PC) whose composition has been analyzed by nanoLC-MS/MS.
28315770	7	24	theme	sandwiched	1179:1188	arg1	pDNA					1174:1177	anionic pDNA	1166:1177	anionic pDNA sandwiched among them	1166:1199	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
28315770	1	25	theme	cellular	90:97	arg1	transfection					99:110	Efficient cellular transfection	80:110	Efficient cellular transfection on cancer cells	80:126	Efficient cellular transfection on cancer cells.
28315770	7	26	theme	anionic	1166:1172	arg1	pDNA					1174:1177	anionic pDNA	1166:1177	anionic pDNA sandwiched among them	1166:1199	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
28315770	3	27	theme	tetradecahexanoyl	409:425	arg1	domain					437:442	a common tetradecahexanoyl multitail domain	400:442	a common tetradecahexanoyl multitail domain	400:442	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	3	28	theme	cluster	503:509	arg1	topology					487:494	the topology	483:494	the topology of the cluster of amino groups at the primary side	483:545	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	5	29	theme	zeta	832:835	arg1	potential					837:845	zeta potential	832:845	zeta potential	832:845	The electrochemical and structural characteristics of the paCD-pDNA complexes (CDplexes) have been studied by using zeta potential, DLS, SAXS, and cryo-TEM.
28315770	8	30	theme	protein	1345:1351	arg1	corona					1353:1358	a protein corona	1343:1358	a protein corona (PC) whose composition has been analyzed by nanoLC-MS/MS	1343:1415	When exposed to human serum, either in the absence or in the presence of pDNA, the surface of the cationic CD-based vector becomes coated by a protein corona (PC) whose composition has been analyzed by nanoLC-MS/MS.
28315770	8	30	theme	protein	1345:1351	arg1	PC					1361:1362	PC	1361:1362	PC	1361:1362	When exposed to human serum, either in the absence or in the presence of pDNA, the surface of the cationic CD-based vector becomes coated by a protein corona (PC) whose composition has been analyzed by nanoLC-MS/MS.
28315770	0	31	theme	protein	15:21	arg1	analysis					30:37	protein corona analysis	15:37	protein corona analysis	15:37	Biophysics and protein corona analysis of Janus cyclodextrin-DNA nanocomplexes.
28315770	9	32	theme	cell	1560:1563	arg1	viabilities					1565:1575	moderate-to-high cell viabilities	1543:1575	moderate-to-high cell viabilities	1543:1575	Some of the CDplexes herein studied showed moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities, as determined by FACS and MTT reduction assays.
28315770	3	33	dep	paCDs	386:390	arg1	bearing					392:398	bearing	392:398	bearing a common tetradecahexanoyl multitail domain at the secondary face	392:464	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	3	33	dep	paCDs	386:390	arg1	differing					470:478	differing	470:478	differing in the topology of the cluster of amino groups at the primary side	470:545	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	10	34	theme	future	1795:1800	arg1	applications					1810:1821	future in vivo applications	1795:1821	future in vivo applications	1795:1821	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	10	35	theme	detail	1658:1663	arg1	picture					1665:1671	a detail picture	1656:1671	a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications	1656:1821	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	0	36	theme	Janus	42:46	arg1	cyclodextrin-DNA					48:63	Janus cyclodextrin-DNA	42:63	Janus cyclodextrin-DNA	42:63	Biophysics and protein corona analysis of Janus cyclodextrin-DNA nanocomplexes.
28315770	2	37	theme	differentiated	200:213	arg1	paCDs					265:269	paCDs	265:269	paCDs	265:269	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	2	37	theme	differentiated	200:213	arg1	cyclodextrins					250:262	facially differentiated ("Janus") polycationic amphiphilic cyclodextrins	191:262	facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers	191:301	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	3	38	theme	amino	514:518	arg1	groups					520:525	amino groups	514:525	amino groups at the primary side	514:545	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	2	39	theme	Janus	217:221	arg1	"					222:222	"Janus"	216:222	"Janus"	216:222	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	10	40	theme	paCD-pDNA-PC	1680:1691	arg1	processes					1705:1713	the paCD-pDNA-PC association processes	1676:1713	the paCD-pDNA-PC association processes	1676:1713	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	2	41	theme	self-assembling	133:147	arg1	processes					149:157	The self-assembling processes	129:157	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers	129:301	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	7	42	theme	lamellar	1107:1114	arg1	stacking					1116:1123	a lamellar stacking	1105:1123	a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them	1105:1199	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
28315770	3	43	theme	primary	534:540	arg1	side					542:545	the primary side	530:545	the primary side	530:545	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	9	44	theme	MTT	1604:1606	arg1	assays					1618:1623	MTT reduction assays	1604:1623	MTT reduction assays	1604:1623	Some of the CDplexes herein studied showed moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities, as determined by FACS and MTT reduction assays.
28315770	4	45	theme	pEGFP-C3	597:604	arg1	DNA					614:616	pEGFP-C3 plasmid DNA	597:616	pEGFP-C3 plasmid DNA	597:616	All of them compact pEGFP-C3 plasmid DNA and promote transfection in HeLa and MCF-7 cells, both in absence and in presence of human serum.
28315770	3	46	from	side	542:545	arg1	cluster					503:509	the cluster	499:509	the cluster of amino groups at the primary side	499:545	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	3	46	from	side	542:545	arg1	groups					520:525	amino groups	514:525	amino groups at the primary side	514:545	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	10	47	theme	processes	1705:1713	arg1	picture					1665:1671	a detail picture	1656:1671	a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications	1656:1821	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	7	48	theme	bilayers	1128:1135	arg1	stacking					1116:1123	a lamellar stacking	1105:1123	a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them	1105:1199	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
28315770	8	49	theme	human	1218:1222	arg1	serum					1224:1228	human serum	1218:1228	human serum	1218:1228	When exposed to human serum, either in the absence or in the presence of pDNA, the surface of the cationic CD-based vector becomes coated by a protein corona (PC) whose composition has been analyzed by nanoLC-MS/MS.
28315770	7	50	theme	crystal	1072:1078	arg1	phases					1080:1085	multilamellar lyotropic liquid crystal phases	1041:1085	multilamellar lyotropic liquid crystal phases	1041:1085	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
28315770	7	51	with	vectors	1153:1159	arg1	pDNA					1174:1177	anionic pDNA	1166:1177	anionic pDNA sandwiched among them	1166:1199	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
28315770	7	52	theme	lyotropic	1055:1063	arg1	phases					1080:1085	multilamellar lyotropic liquid crystal phases	1041:1085	multilamellar lyotropic liquid crystal phases	1041:1085	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
28315770	10	53	theme	base	1730:1733	arg1	picture					1665:1671	a detail picture	1656:1671	a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications	1656:1821	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	6	54	theme	effective	924:932	arg1	charges					934:940	effective charges	924:940	effective charges that are lower than the nominal ones	924:977	paCDs and pDNA, when assembled in CDplexes, render effective charges that are lower than the nominal ones.
28315770	10	55	theme	protein	1750:1756	arg1	corona					1758:1763	the protein corona	1746:1763	the protein corona for targeted gene delivery on future in vivo applications	1746:1821	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	4	56	theme	serum	709:713	arg1	presence					691:698	presence	691:698	presence of human serum	691:713	All of them compact pEGFP-C3 plasmid DNA and promote transfection in HeLa and MCF-7 cells, both in absence and in presence of human serum.
28315770	2	57	theme	gene	285:288	arg1	nanocarriers					290:301	non-viral gene nanocarriers	275:301	non-viral gene nanocarriers	275:301	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	10	58	theme	gene	1778:1781	arg1	delivery					1783:1790	targeted gene delivery	1769:1790	targeted gene delivery	1769:1790	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	3	59	theme	Janus	380:384	arg1	paCDs					386:390	Three representative Janus paCDs	359:390	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side	359:545	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	8	60	theme	pDNA	1275:1278	arg1	presence					1263:1270	the presence	1259:1270	the presence of pDNA	1259:1278	When exposed to human serum, either in the absence or in the presence of pDNA, the surface of the cationic CD-based vector becomes coated by a protein corona (PC) whose composition has been analyzed by nanoLC-MS/MS.
28315770	4	61	from	transfection	630:641	arg1	cells					661:665	HeLa and MCF-7 cells	646:665	HeLa and MCF-7 cells	646:665	All of them compact pEGFP-C3 plasmid DNA and promote transfection in HeLa and MCF-7 cells, both in absence and in presence of human serum.
28315770	4	61	from	transfection	630:641	arg1	absence					676:682	absence	676:682	absence	676:682	All of them compact pEGFP-C3 plasmid DNA and promote transfection in HeLa and MCF-7 cells, both in absence and in presence of human serum.
28315770	4	61	from	transfection	630:641	arg1	presence					691:698	presence	691:698	presence of human serum	691:713	All of them compact pEGFP-C3 plasmid DNA and promote transfection in HeLa and MCF-7 cells, both in absence and in presence of human serum.
28315770	5	62	theme	complexes	784:792	arg1	characteristics					751:765	The electrochemical and structural characteristics	716:765	The electrochemical and structural characteristics of the paCD-pDNA complexes (CDplexes)	716:803	The electrochemical and structural characteristics of the paCD-pDNA complexes (CDplexes) have been studied by using zeta potential, DLS, SAXS, and cryo-TEM.
28315770	9	63	theme	transfection	1478:1489	arg1	levels					1491:1496	moderate-to-high transfection levels	1461:1496	moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities	1461:1575	Some of the CDplexes herein studied showed moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities, as determined by FACS and MTT reduction assays.
28315770	3	64	theme	common	402:407	arg1	domain					437:442	a common tetradecahexanoyl multitail domain	400:442	a common tetradecahexanoyl multitail domain	400:442	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	3	65	theme	groups	520:525	arg1	cluster					503:509	the cluster	499:509	the cluster of amino groups at the primary side	499:545	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	1	66	theme	Efficient	80:88	arg1	transfection					99:110	Efficient cellular transfection	80:110	Efficient cellular transfection on cancer cells	80:126	Efficient cellular transfection on cancer cells.
28315770	8	67	theme	cationic	1300:1307	arg1	vector					1318:1323	the cationic CD-based vector	1296:1323	the cationic CD-based vector	1296:1323	When exposed to human serum, either in the absence or in the presence of pDNA, the surface of the cationic CD-based vector becomes coated by a protein corona (PC) whose composition has been analyzed by nanoLC-MS/MS.
28315770	9	68	theme	cancer	1516:1521	arg1	cells					1523:1527	HeLa and MCF-7 cancer cells	1501:1527	HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities	1501:1575	Some of the CDplexes herein studied showed moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities, as determined by FACS and MTT reduction assays.
28315770	10	69	from	corona	1758:1763	arg1	applications					1810:1821	future in vivo applications	1795:1821	future in vivo applications	1795:1821	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	2	70	theme	cyclodextrins	250:262	arg1	capabilities					175:186	the capabilities	171:186	the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers	171:301	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	8	71	theme	vector	1318:1323	arg1	surface					1285:1291	the surface	1281:1291	the surface of the cationic CD-based vector	1281:1323	When exposed to human serum, either in the absence or in the presence of pDNA, the surface of the cationic CD-based vector becomes coated by a protein corona (PC) whose composition has been analyzed by nanoLC-MS/MS.
28315770	0	72	theme	corona	23:28	arg1	analysis					30:37	protein corona analysis	15:37	protein corona analysis	15:37	Biophysics and protein corona analysis of Janus cyclodextrin-DNA nanocomplexes.
28315770	1	73	theme	cancer	115:120	arg1	cells					122:126	cancer cells	115:126	cancer cells	115:126	Efficient cellular transfection on cancer cells.
28315770	3	74	theme	multitail	427:435	arg1	domain					437:442	a common tetradecahexanoyl multitail domain	400:442	a common tetradecahexanoyl multitail domain	400:442	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	9	75	theme	moderate-to-high	1543:1558	arg1	viabilities					1565:1575	moderate-to-high cell viabilities	1543:1575	moderate-to-high cell viabilities	1543:1575	Some of the CDplexes herein studied showed moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities, as determined by FACS and MTT reduction assays.
28315770	10	76	theme	in	1802:1803	arg1	applications					1810:1821	future in vivo applications	1795:1821	future in vivo applications	1795:1821	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	0	77	theme	cyclodextrin-DNA	48:63	arg1	Biophysics					0:9	Biophysics	0:9	Biophysics	0:9	Biophysics and protein corona analysis of Janus cyclodextrin-DNA nanocomplexes.
28315770	0	77	theme	cyclodextrin-DNA	48:63	arg1	analysis					30:37	protein corona analysis	15:37	protein corona analysis	15:37	Biophysics and protein corona analysis of Janus cyclodextrin-DNA nanocomplexes.
28315770	3	78	theme	secondary	451:459	arg1	face					461:464	the secondary face	447:464	the secondary face	447:464	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	2	79	theme	polycationic	225:236	arg1	paCDs					265:269	paCDs	265:269	paCDs	265:269	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	2	79	theme	polycationic	225:236	arg1	cyclodextrins					250:262	facially differentiated ("Janus") polycationic amphiphilic cyclodextrins	191:262	facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers	191:301	The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach.
28315770	3	80	from	cluster	503:509	arg1	side					542:545	the primary side	530:545	the primary side	530:545	Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study.
28315770	10	81	dep	in	1802:1803	arg1	vivo					1805:1808	vivo	1805:1808	vivo	1805:1808	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	9	82	theme	MCF-7	1510:1514	arg1	cells					1523:1527	HeLa and MCF-7 cancer cells	1501:1527	HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities	1501:1575	Some of the CDplexes herein studied showed moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities, as determined by FACS and MTT reduction assays.
28315770	10	83	theme	data	1642:1645	arg1	ensemble					1630:1637	The ensemble	1626:1637	The ensemble of data	1626:1645	The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications.
28315770	1	84	from	transfection	99:110	arg1	cells					122:126	cancer cells	115:126	cancer cells	115:126	Efficient cellular transfection on cancer cells.
28315770	4	85	theme	HeLa	646:649	arg1	cells					661:665	HeLa and MCF-7 cells	646:665	HeLa and MCF-7 cells	646:665	All of them compact pEGFP-C3 plasmid DNA and promote transfection in HeLa and MCF-7 cells, both in absence and in presence of human serum.
28315770	7	86	theme	vectors	1153:1159	arg1	bilayers					1128:1135	bilayers	1128:1135	bilayers of the CD-based vectors with anionic pDNA sandwiched among them	1128:1199	The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them.
23995918	10	0	theme	industrial	1522:1531	arg1	requirements					1533:1544	consumer and industrial requirements	1509:1544	consumer and industrial requirements	1509:1544	Consequently, targeted plant inoculation with beneficial microorganisms can lead to commodities fulfilling consumer and industrial requirements.
23995918	7	1	theme	fungi-enhanced	1049:1062	arg1	zein					1084:1087	zein	1084:1087	zein	1084:1087	Regarding grain composition, the bacterial strain increased grain starch content, especially the digestible components, whereas AM fungi-enhanced protein, especially zein, content.
23995918	7	1	theme	fungi-enhanced	1049:1062	arg1	protein					1064:1070	fungi-enhanced protein	1049:1070	fungi-enhanced protein	1049:1070	Regarding grain composition, the bacterial strain increased grain starch content, especially the digestible components, whereas AM fungi-enhanced protein, especially zein, content.
23995918	4	2	from	content	700:706	arg1	grains					711:716	grains	711:716	grains	711:716	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	3	3	from	growth	497:502	arg1	conditions					518:527	open-field conditions	507:527	open-field conditions	507:527	Plants were inoculated with AM fungi and/or the bacterial strain Pseudomonas fluorescens Pf4 and harvested after 7 months of growth in open-field conditions.
23995918	4	4	theme	grain	640:644	arg1	yield					646:650	grain yield	640:650	grain yield	640:650	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	4	theme	grain	640:644	arg1	biomass					569:575	biomass	569:575	biomass	569:575	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	0	5	theme	growth-promoting	91:106	arg1	pseudomonad					108:118	a growth-promoting pseudomonad	89:118	a growth-promoting pseudomonad in the field	89:131	Maize development and grain quality are differentially affected by mycorrhizal fungi and a growth-promoting pseudomonad in the field.
23995918	6	6	theme	inoculated	844:853	arg1	plants					855:860	inoculated plants	844:860	inoculated plants	844:860	Moreover, spikes and grains of inoculated plants were bigger than those produced by uninoculated plants.
23995918	9	7	theme	grain	1375:1379	arg1	content					1393:1399	the grain nutritional content	1371:1399	the grain nutritional content	1371:1399	Overall, results showed that the bacterial strain and the AM fungi promoted maize growth cultivated in field conditions and differentially affected the grain nutritional content.
23995918	3	8	dep	Pseudomonas	437:447	arg1	fluorescens					449:459	fluorescens	449:459	fluorescens	449:459	Plants were inoculated with AM fungi and/or the bacterial strain Pseudomonas fluorescens Pf4 and harvested after 7 months of growth in open-field conditions.
23995918	3	9	theme	open-field	507:516	arg1	conditions					518:527	open-field conditions	507:527	open-field conditions	507:527	Plants were inoculated with AM fungi and/or the bacterial strain Pseudomonas fluorescens Pf4 and harvested after 7 months of growth in open-field conditions.
23995918	1	10	theme	major	248:252	arg1	crops					254:258	major crops	248:258	major crops	248:258	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	9	11	theme	bacterial	1256:1264	arg1	strain					1266:1271	the bacterial strain	1252:1271	the bacterial strain	1252:1271	Overall, results showed that the bacterial strain and the AM fungi promoted maize growth cultivated in field conditions and differentially affected the grain nutritional content.
23995918	3	12	from	7 months	485:492	arg1	conditions					518:527	open-field conditions	507:527	open-field conditions	507:527	Plants were inoculated with AM fungi and/or the bacterial strain Pseudomonas fluorescens Pf4 and harvested after 7 months of growth in open-field conditions.
23995918	4	13	theme	starch	693:698	arg1	content					700:706	protein, lipid, and starch content	673:706	content	700:706	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	8	14	theme	additive	1184:1191	arg1	effects					1193:1199	additive effects	1184:1199	additive effects on grain composition	1184:1220	Plant inoculation with the fluorescent pseudomonad and mycorrhizal fungi resulted in additive effects on grain composition.
23995918	6	15	theme	uninoculated	897:908	arg1	plants					910:915	uninoculated plants	897:915	uninoculated plants	897:915	Moreover, spikes and grains of inoculated plants were bigger than those produced by uninoculated plants.
23995918	8	16	with	inoculation	1105:1115	arg1	pseudomonad					1138:1148	fluorescent pseudomonad	1126:1148	fluorescent pseudomonad	1126:1148	Plant inoculation with the fluorescent pseudomonad and mycorrhizal fungi resulted in additive effects on grain composition.
23995918	8	16	with	inoculation	1105:1115	arg1	fungi					1166:1170	mycorrhizal fungi	1154:1170	mycorrhizal fungi	1154:1170	Plant inoculation with the fluorescent pseudomonad and mycorrhizal fungi resulted in additive effects on grain composition.
23995918	3	17	theme	growth	497:502	arg1	7 months					485:492	7 months	485:492	7 months of growth in open-field conditions	485:527	Plants were inoculated with AM fungi and/or the bacterial strain Pseudomonas fluorescens Pf4 and harvested after 7 months of growth in open-field conditions.
23995918	3	18	theme	AM	400:401	arg1	fungi					403:407	AM fungi	400:407	AM fungi	400:407	Plants were inoculated with AM fungi and/or the bacterial strain Pseudomonas fluorescens Pf4 and harvested after 7 months of growth in open-field conditions.
23995918	10	19	theme	plant	1425:1429	arg1	inoculation					1431:1441	targeted plant inoculation	1416:1441	targeted plant inoculation with beneficial microorganisms	1416:1472	Consequently, targeted plant inoculation with beneficial microorganisms can lead to commodities fulfilling consumer and industrial requirements.
23995918	4	20	dep	measured	559:566	arg1	circumference					589:601	circumference	589:601	circumference	589:601	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	20	dep	measured	559:566	arg1	size					663:666	grain size	657:666	grain size	657:666	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	20	dep	measured	559:566	arg1	biomass					569:575	biomass	569:575	biomass	569:575	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	20	dep	measured	559:566	arg1	yield					646:650	grain yield	640:650	grain yield	640:650	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	20	dep	measured	559:566	arg1	length					578:583	length	578:583	length	578:583	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	20	dep	measured	559:566	arg1	number					614:619	number	614:619	number of grains per cob	614:637	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	1	21	theme	plant	172:176	arg1	PGPB					205:208	PGPB	205:208	PGPB	205:208	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	1	21	theme	plant	172:176	arg1	bacteria					195:202	plant growth-promoting bacteria	172:202	plant growth-promoting bacteria (PGPB)	172:209	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	0	22	theme	Maize	0:4	arg1	development					6:16	Maize development	0:16	Maize development	0:16	Maize development and grain quality are differentially affected by mycorrhizal fungi and a growth-promoting pseudomonad in the field.
23995918	1	23	theme	crops	254:258	arg1	yield					239:243	yield	239:243	yield	239:243	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	1	23	theme	crops	254:258	arg1	growth					228:233	the growth	224:233	the growth	224:233	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	4	24	theme	grain	657:661	arg1	biomass					569:575	biomass	569:575	biomass	569:575	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	24	theme	grain	657:661	arg1	size					663:666	grain size	657:666	grain size	657:666	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	8	25	theme	grain	1204:1208	arg1	composition					1210:1220	grain composition	1204:1220	grain composition	1204:1220	Plant inoculation with the fluorescent pseudomonad and mycorrhizal fungi resulted in additive effects on grain composition.
23995918	1	26	theme	growth-promoting	178:193	arg1	PGPB					205:208	PGPB	205:208	PGPB	205:208	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	1	26	theme	growth-promoting	178:193	arg1	bacteria					195:202	plant growth-promoting bacteria	172:202	plant growth-promoting bacteria (PGPB)	172:209	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	0	27	theme	grain	22:26	arg1	quality					28:34	grain quality	22:34	grain quality	22:34	Maize development and grain quality are differentially affected by mycorrhizal fungi and a growth-promoting pseudomonad in the field.
23995918	8	28	theme	Plant	1099:1103	arg1	inoculation					1105:1115	Plant inoculation	1099:1115	Plant inoculation with the fluorescent pseudomonad and mycorrhizal fungi	1099:1170	Plant inoculation with the fluorescent pseudomonad and mycorrhizal fungi resulted in additive effects on grain composition.
23995918	4	29	from	lipid	682:686	arg1	grains					711:716	grains	711:716	grains	711:716	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	10	30	theme	targeted	1416:1423	arg1	inoculation					1431:1441	targeted plant inoculation	1416:1441	targeted plant inoculation with beneficial microorganisms	1416:1472	Consequently, targeted plant inoculation with beneficial microorganisms can lead to commodities fulfilling consumer and industrial requirements.
23995918	8	31	theme	fluorescent	1126:1136	arg1	pseudomonad					1138:1148	fluorescent pseudomonad	1126:1148	fluorescent pseudomonad	1126:1148	Plant inoculation with the fluorescent pseudomonad and mycorrhizal fungi resulted in additive effects on grain composition.
23995918	4	32	theme	growth	536:541	arg1	parameters					543:552	Plant growth parameters	530:552	Plant growth parameters	530:552	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	33	theme	protein	673:679	arg1	lipid					682:686	protein, lipid, and starch content	673:706	lipid	682:686	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	9	34	theme	nutritional	1381:1391	arg1	content					1393:1399	the grain nutritional content	1371:1399	the grain nutritional content	1371:1399	Overall, results showed that the bacterial strain and the AM fungi promoted maize growth cultivated in field conditions and differentially affected the grain nutritional content.
23995918	3	35	theme	bacterial	420:428	arg1	strain					430:435	the bacterial strain	416:435	the bacterial strain	416:435	Plants were inoculated with AM fungi and/or the bacterial strain Pseudomonas fluorescens Pf4 and harvested after 7 months of growth in open-field conditions.
23995918	8	36	theme	mycorrhizal	1154:1164	arg1	fungi					1166:1170	mycorrhizal fungi	1154:1170	mycorrhizal fungi	1154:1170	Plant inoculation with the fluorescent pseudomonad and mycorrhizal fungi resulted in additive effects on grain composition.
23995918	4	37	theme	Plant	530:534	arg1	parameters					543:552	Plant growth parameters	530:552	Plant growth parameters	530:552	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	7	38	theme	starch	984:989	arg1	components					1026:1035	the digestible components	1011:1035	especially the digestible components	1000:1035	Regarding grain composition, the bacterial strain increased grain starch content, especially the digestible components, whereas AM fungi-enhanced protein, especially zein, content.
23995918	7	38	theme	starch	984:989	arg1	content					991:997	grain starch content	978:997	grain starch content	978:997	Regarding grain composition, the bacterial strain increased grain starch content, especially the digestible components, whereas AM fungi-enhanced protein, especially zein, content.
23995918	0	39	from	fungi	79:83	arg1	field					127:131	the field	123:131	the field	123:131	Maize development and grain quality are differentially affected by mycorrhizal fungi and a growth-promoting pseudomonad in the field.
23995918	10	40	theme	consumer	1509:1516	arg1	requirements					1533:1544	consumer and industrial requirements	1509:1544	consumer and industrial requirements	1509:1544	Consequently, targeted plant inoculation with beneficial microorganisms can lead to commodities fulfilling consumer and industrial requirements.
23995918	4	41	theme	grains	624:629	arg1	yield					646:650	grain yield	640:650	grain yield	640:650	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	41	theme	grains	624:629	arg1	biomass					569:575	biomass	569:575	biomass	569:575	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	41	theme	grains	624:629	arg1	number					614:619	number	614:619	number of grains per cob	614:637	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	41	theme	grains	624:629	arg1	size					663:666	grain size	657:666	grain size	657:666	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	9	42	theme	maize	1299:1303	arg1	growth					1305:1310	maize growth	1299:1310	maize growth cultivated in field conditions	1299:1341	Overall, results showed that the bacterial strain and the AM fungi promoted maize growth cultivated in field conditions and differentially affected the grain nutritional content.
23995918	10	43	theme	beneficial	1448:1457	arg1	microorganisms					1459:1472	beneficial microorganisms	1448:1472	beneficial microorganisms	1448:1472	Consequently, targeted plant inoculation with beneficial microorganisms can lead to commodities fulfilling consumer and industrial requirements.
23995918	5	44	theme	Plant	735:739	arg1	growth					741:746	Plant growth	735:746	Plant growth	735:746	Plant growth and yield were increased by inoculation with the microorganisms.
23995918	2	45	theme	seed	354:357	arg1	composition					359:369	seed composition	354:369	seed composition	354:369	However, little is known about their impact on seed composition.
23995918	8	46	dep	pseudomonad	1138:1148	arg1	the					1122:1124	the	1122:1124	the	1122:1124	Plant inoculation with the fluorescent pseudomonad and mycorrhizal fungi resulted in additive effects on grain composition.
23995918	9	47	theme	AM	1281:1282	arg1	fungi					1284:1288	the AM fungi	1277:1288	the AM fungi	1277:1288	Overall, results showed that the bacterial strain and the AM fungi promoted maize growth cultivated in field conditions and differentially affected the grain nutritional content.
23995918	8	48	from	effects	1193:1199	arg1	composition					1210:1220	grain composition	1204:1220	grain composition	1204:1220	Plant inoculation with the fluorescent pseudomonad and mycorrhizal fungi resulted in additive effects on grain composition.
23995918	6	49	theme	plants	855:860	arg1	grains					834:839	grains	834:839	grains	834:839	Moreover, spikes and grains of inoculated plants were bigger than those produced by uninoculated plants.
23995918	6	49	theme	plants	855:860	arg1	spikes					823:828	spikes	823:828	spikes	823:828	Moreover, spikes and grains of inoculated plants were bigger than those produced by uninoculated plants.
23995918	7	50	theme	grain	978:982	arg1	components					1026:1035	the digestible components	1011:1035	especially the digestible components	1000:1035	Regarding grain composition, the bacterial strain increased grain starch content, especially the digestible components, whereas AM fungi-enhanced protein, especially zein, content.
23995918	7	50	theme	grain	978:982	arg1	content					991:997	grain starch content	978:997	grain starch content	978:997	Regarding grain composition, the bacterial strain increased grain starch content, especially the digestible components, whereas AM fungi-enhanced protein, especially zein, content.
23995918	7	51	theme	grain	928:932	arg1	composition					934:944	grain composition	928:944	grain composition	928:944	Regarding grain composition, the bacterial strain increased grain starch content, especially the digestible components, whereas AM fungi-enhanced protein, especially zein, content.
23995918	3	52	from	conditions	518:527	arg1	7 months					485:492	7 months	485:492	7 months of growth in open-field conditions	485:527	Plants were inoculated with AM fungi and/or the bacterial strain Pseudomonas fluorescens Pf4 and harvested after 7 months of growth in open-field conditions.
23995918	1	53	theme	fruits	288:293	arg1	quality					277:283	the quality	273:283	the quality of fruits	273:293	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	7	54	theme	bacterial	951:959	arg1	strain					961:966	the bacterial strain	947:966	the bacterial strain	947:966	Regarding grain composition, the bacterial strain increased grain starch content, especially the digestible components, whereas AM fungi-enhanced protein, especially zein, content.
23995918	0	55	from	pseudomonad	108:118	arg1	field					127:131	the field	123:131	the field	123:131	Maize development and grain quality are differentially affected by mycorrhizal fungi and a growth-promoting pseudomonad in the field.
23995918	3	56	dep	fungi	403:407	arg1	Pf4					461:463	Pseudomonas fluorescens Pf4	437:463	Pseudomonas fluorescens Pf4	437:463	Plants were inoculated with AM fungi and/or the bacterial strain Pseudomonas fluorescens Pf4 and harvested after 7 months of growth in open-field conditions.
23995918	2	57	from	impact	344:349	arg1	composition					359:369	seed composition	354:369	seed composition	354:369	However, little is known about their impact on seed composition.
23995918	9	58	theme	field	1326:1330	arg1	conditions					1332:1341	field conditions	1326:1341	field conditions	1326:1341	Overall, results showed that the bacterial strain and the AM fungi promoted maize growth cultivated in field conditions and differentially affected the grain nutritional content.
23995918	5	59	with	inoculation	776:786	arg1	microorganisms					797:810	the microorganisms	793:810	the microorganisms	793:810	Plant growth and yield were increased by inoculation with the microorganisms.
23995918	10	60	with	inoculation	1431:1441	arg1	microorganisms					1459:1472	beneficial microorganisms	1448:1472	beneficial microorganisms	1448:1472	Consequently, targeted plant inoculation with beneficial microorganisms can lead to commodities fulfilling consumer and industrial requirements.
23995918	1	61	theme	Arbuscular	134:143	arg1	fungi					162:166	Arbuscular mycorrhizal (AM) fungi	134:166	Arbuscular mycorrhizal (AM) fungi	134:166	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	1	61	theme	Arbuscular	134:143	arg1	AM					158:159	AM	158:159	AM	158:159	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	7	62	theme	digestible	1015:1024	arg1	components					1026:1035	the digestible components	1011:1035	especially the digestible components	1000:1035	Regarding grain composition, the bacterial strain increased grain starch content, especially the digestible components, whereas AM fungi-enhanced protein, especially zein, content.
23995918	7	62	theme	digestible	1015:1024	arg1	content					991:997	grain starch content	978:997	grain starch content	978:997	Regarding grain composition, the bacterial strain increased grain starch content, especially the digestible components, whereas AM fungi-enhanced protein, especially zein, content.
23995918	0	63	theme	mycorrhizal	67:77	arg1	fungi					79:83	mycorrhizal fungi	67:83	mycorrhizal fungi	67:83	Maize development and grain quality are differentially affected by mycorrhizal fungi and a growth-promoting pseudomonad in the field.
23995918	4	64	theme	spikes	606:611	arg1	circumference					589:601	circumference	589:601	circumference	589:601	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	64	theme	spikes	606:611	arg1	size					663:666	grain size	657:666	grain size	657:666	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	64	theme	spikes	606:611	arg1	biomass					569:575	biomass	569:575	biomass	569:575	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	64	theme	spikes	606:611	arg1	yield					646:650	grain yield	640:650	grain yield	640:650	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	64	theme	spikes	606:611	arg1	length					578:583	length	578:583	length	578:583	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	4	64	theme	spikes	606:611	arg1	number					614:619	number	614:619	number of grains per cob	614:637	Plant growth parameters were measured (biomass, length and circumference of spikes, number of grains per cob, grain yield, and grain size) and protein, lipid, and starch content in grains were determined.
23995918	1	65	theme	mycorrhizal	145:155	arg1	fungi					162:166	Arbuscular mycorrhizal (AM) fungi	134:166	Arbuscular mycorrhizal (AM) fungi	134:166	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	1	65	theme	mycorrhizal	145:155	arg1	AM					158:159	AM	158:159	AM	158:159	Arbuscular mycorrhizal (AM) fungi and plant growth-promoting bacteria (PGPB) can increase the growth and yield of major crops, and improve the quality of fruits and leaves.
23995918	3	66	theme	Pseudomonas	437:447	arg1	Pf4					461:463	Pseudomonas fluorescens Pf4	437:463	Pseudomonas fluorescens Pf4	437:463	Plants were inoculated with AM fungi and/or the bacterial strain Pseudomonas fluorescens Pf4 and harvested after 7 months of growth in open-field conditions.
27922059	2	0	theme	microtubules	524:535	arg1	filaments					502:510	the filaments	498:510	the filaments of cellular microtubules	498:535	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	3	1	theme	hybrids	753:759	arg1	properties					670:679	The physical and chemical properties	644:679	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids	644:759	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	1	2	theme	increased	269:277	arg1	ability					279:285	increased ability	269:285	increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement	269:377	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
27922059	0	3	theme	hybrids	79:85	arg1	formation					45:53	formation	45:53	formation of conductive bio-based hybrids	45:85	Protein self-assembly onto nanodots leads to formation of conductive bio-based hybrids.
27922059	1	4	theme	functional	159:168	arg1	nanosystems					170:180	functional nanosystems	159:180	functional nanosystems	159:180	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
27922059	4	5	theme	functional	1144:1153	arg1	devices					1155:1161	nanometer-scale functional devices	1128:1161	nanometer-scale functional devices	1128:1161	Our results demonstrate the ability to create individually hybrid nanowires capable to reduce energy losses; such hybrids could possibly be used in the future for the advancement and implementation into nanometer-scale functional devices.
27922059	4	6	theme	capable	1001:1007	arg1	nanowires					991:999	hybrid nanowires	984:999	hybrid nanowires capable to reduce energy losses	984:1031	Our results demonstrate the ability to create individually hybrid nanowires capable to reduce energy losses; such hybrids could possibly be used in the future for the advancement and implementation into nanometer-scale functional devices.
27922059	3	7	theme	such	731:734	arg1	hybrids					753:759	such user-synthesized hybrids	731:759	such user-synthesized hybrids	731:759	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	1	8	from	interfaces	325:334	arg1	transfer					307:314	electron transfer	298:314	electron transfer at their interfaces	298:334	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
27922059	2	9	used	used	390:393	arg2	we					387:388	we	387:388	we	387:388	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	4	10	theme	nanometer-scale	1128:1142	arg1	devices					1155:1161	nanometer-scale functional devices	1128:1161	nanometer-scale functional devices	1128:1161	Our results demonstrate the ability to create individually hybrid nanowires capable to reduce energy losses; such hybrids could possibly be used in the future for the advancement and implementation into nanometer-scale functional devices.
27922059	3	11	theme	peak	875:878	arg1	currents					880:887	peak currents	875:887	peak currents formed during voltammetry scanning	875:922	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	4	12	theme	energy	1019:1024	arg1	losses					1026:1031	energy losses	1019:1031	energy losses	1019:1031	Our results demonstrate the ability to create individually hybrid nanowires capable to reduce energy losses; such hybrids could possibly be used in the future for the advancement and implementation into nanometer-scale functional devices.
27922059	2	13	theme	stable	548:553	arg1	standing					561:568	stable, free standing	548:568	stable, free standing	548:568	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	3	14	theme	atomic	785:790	arg1	techniques					810:819	atomic and spectroscopic techniques	785:819	atomic and spectroscopic techniques	785:819	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	2	15	dep	stable	548:553	arg1	free					556:559	free	556:559	free	556:559	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	1	16	theme	nanosystems	170:180	arg1	integration					144:154	the integration	140:154	the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices	140:247	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
27922059	4	17	used	used	1065:1068	arg2	hybrids					1039:1045	such hybrids	1034:1045	such hybrids	1034:1045	Our results demonstrate the ability to create individually hybrid nanowires capable to reduce energy losses; such hybrids could possibly be used in the future for the advancement and implementation into nanometer-scale functional devices.
27922059	4	18	dep	advancement	1092:1102	arg1	the					1088:1090	the	1088:1090	the	1088:1090	Our results demonstrate the ability to create individually hybrid nanowires capable to reduce energy losses; such hybrids could possibly be used in the future for the advancement and implementation into nanometer-scale functional devices.
27922059	4	19	theme	such	1034:1037	arg1	hybrids					1039:1045	such hybrids	1034:1045	such hybrids	1034:1045	Our results demonstrate the ability to create individually hybrid nanowires capable to reduce energy losses; such hybrids could possibly be used in the future for the advancement and implementation into nanometer-scale functional devices.
27922059	2	20	theme	cellular	515:522	arg1	microtubules					524:535	cellular microtubules	515:535	cellular microtubules	515:535	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	1	21	theme	synthetic	187:195	arg1	applications					197:208	synthetic applications	187:208	synthetic applications from photocatalysis to optical devices	187:247	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
27922059	0	22	theme	Protein	0:6	arg1	self-assembly					8:20	Protein self-assembly	0:20	Protein self-assembly onto nanodots	0:34	Protein self-assembly onto nanodots leads to formation of conductive bio-based hybrids.
27922059	1	23	theme	electron	298:305	arg1	transfer					307:314	electron transfer	298:314	electron transfer at their interfaces	298:334	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
27922059	1	24	theme	next	92:95	arg1	generation					97:106	The next generation	88:106	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices	88:247	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
27922059	2	25	theme	sulfur-doped	585:596	arg1	bio-hybrids					631:641	conductive sulfur-doped carbon nanodots-based conductive bio-hybrids	574:641	conductive sulfur-doped carbon nanodots-based conductive bio-hybrids	574:641	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	2	26	theme	conductive	574:583	arg1	bio-hybrids					631:641	conductive sulfur-doped carbon nanodots-based conductive bio-hybrids	574:641	conductive sulfur-doped carbon nanodots-based conductive bio-hybrids	574:641	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	2	27	theme	tubulin	458:464	arg1	recognition					410:420	the biological recognition	395:420	the biological recognition	395:420	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	2	27	theme	tubulin	458:464	arg1	properties					444:453	the self-assembly properties	426:453	the self-assembly properties	426:453	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	3	28	theme	spectroscopic	796:808	arg1	techniques					810:819	atomic and spectroscopic techniques	785:819	atomic and spectroscopic techniques	785:819	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	3	29	theme	physical	648:655	arg1	properties					670:679	The physical and chemical properties	644:679	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids	644:759	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	2	30	theme	self-assembly	430:442	arg1	properties					444:453	the self-assembly properties	426:453	the self-assembly properties	426:453	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	3	31	theme	electron	832:839	arg1	rate					850:853	the electron transfer rate	828:853	the electron transfer rate	828:853	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	1	32	theme	optical	233:239	arg1	devices					241:247	optical devices	233:247	optical devices	233:247	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
27922059	3	33	theme	transfer	841:848	arg1	rate					850:853	the electron transfer rate	828:853	the electron transfer rate	828:853	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	4	34	theme	hybrid	984:989	arg1	nanowires					991:999	hybrid nanowires	984:999	hybrid nanowires capable to reduce energy losses	984:1031	Our results demonstrate the ability to create individually hybrid nanowires capable to reduce energy losses; such hybrids could possibly be used in the future for the advancement and implementation into nanometer-scale functional devices.
27922059	1	35	theme	nanowires	111:119	arg1	generation					97:106	The next generation	88:106	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices	88:247	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
27922059	2	36	theme	conductive	620:629	arg1	bio-hybrids					631:641	conductive sulfur-doped carbon nanodots-based conductive bio-hybrids	574:641	conductive sulfur-doped carbon nanodots-based conductive bio-hybrids	574:641	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	3	37	theme	user-synthesized	736:751	arg1	hybrids					753:759	such user-synthesized hybrids	731:759	such user-synthesized hybrids	731:759	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	1	38	theme	optimum	351:357	arg1	confinement					367:377	optimum quantum confinement	351:377	optimum quantum confinement	351:377	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
27922059	2	39	theme	nanodots-based	605:618	arg1	bio-hybrids					631:641	conductive sulfur-doped carbon nanodots-based conductive bio-hybrids	574:641	conductive sulfur-doped carbon nanodots-based conductive bio-hybrids	574:641	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	1	40	from	photocatalysis	215:228	arg1	applications					197:208	synthetic applications	187:208	synthetic applications from photocatalysis to optical devices	187:247	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
27922059	2	41	theme	carbon	598:603	arg1	bio-hybrids					631:641	conductive sulfur-doped carbon nanodots-based conductive bio-hybrids	574:641	conductive sulfur-doped carbon nanodots-based conductive bio-hybrids	574:641	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	3	42	theme	voltammetry	903:913	arg1	scanning					915:922	voltammetry scanning	903:922	voltammetry scanning	903:922	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	3	43	dep	composition	688:698	arg1	e.g.					682:685	e.g.	682:685	e.g.	682:685	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	0	44	theme	bio-based	69:77	arg1	hybrids					79:85	conductive bio-based hybrids	58:85	conductive bio-based hybrids	58:85	Protein self-assembly onto nanodots leads to formation of conductive bio-based hybrids.
27922059	3	45	theme	diameter	713:720	arg1	etc.					722:725	diameter etc.	713:725	diameter etc.	713:725	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	3	45	theme	diameter	713:720	arg1	composition					688:698	composition	688:698	composition	688:698	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	3	46	theme	chemical	661:668	arg1	properties					670:679	The physical and chemical properties	644:679	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids	644:759	The physical and chemical properties (e.g., composition, morphology, diameter etc.) of such user-synthesized hybrids were investigated using atomic and spectroscopic techniques, while the electron transfer rate was estimated using peak currents formed during voltammetry scanning.
27922059	2	47	theme	biological	399:408	arg1	recognition					410:420	the biological recognition	395:420	the biological recognition	395:420	Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids.
27922059	0	48	theme	conductive	58:67	arg1	hybrids					79:85	conductive bio-based hybrids	58:85	conductive bio-based hybrids	58:85	Protein self-assembly onto nanodots leads to formation of conductive bio-based hybrids.
27922059	1	49	theme	quantum	359:365	arg1	confinement					367:377	optimum quantum confinement	351:377	optimum quantum confinement	351:377	The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement.
25215315	5	0	theme	copper	859:864	arg1	sulphate					889:896	zinc sulphate	884:896	zinc sulphate (ZnSO4)	884:904	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	0	theme	copper	859:864	arg1	Cu2O					873:876	Cu2O	873:876	Cu2O	873:876	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	0	theme	copper	859:864	arg1	oxide					866:870	copper oxide	859:870	copper oxide (Cu2O)	859:877	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	0	theme	copper	859:864	arg1	material					920:927	the target material	909:927	the target material	909:927	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	0	theme	copper	859:864	arg1	nanotubes					840:848	amorphous carbon nanotubes	823:848	amorphous carbon nanotubes (α-CNT)	823:856	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	1	theme	capped	767:772	arg1	AuNPs					774:778	chitosan capped AuNPs	758:778	chitosan capped AuNPs	758:778	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	0	2	with	nanoparticles	79:91	arg1	analytes					118:125	analytes	118:125	analytes	118:125	Synthesis and ultraviolet visible spectroscopy studies of chitosan capped gold nanoparticles and their reactions with analytes.
25215315	6	3	theme	absorption	934:943	arg1	spectra					945:951	The absorption spectra	930:951	The absorption spectra	930:951	The absorption spectra showed dramatic intensity increased and red shifted once the analyte was added to the chitosan capped AuNPs.
25215315	2	4	theme	resonance	527:535	arg1	peak					559:562	the surface plasmon resonance (SPR) characteristics peak	507:562	the surface plasmon resonance (SPR) characteristics peak	507:562	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	2	5	theme	microscopy	389:398	arg1	images					406:411	transmission electron microscopy (TEM) images	367:411	transmission electron microscopy (TEM) images	367:411	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	5	6	theme	carbon	833:838	arg1	sulphate					889:896	zinc sulphate	884:896	zinc sulphate (ZnSO4)	884:904	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	6	theme	carbon	833:838	arg1	nanotubes					840:848	amorphous carbon nanotubes	823:848	amorphous carbon nanotubes (α-CNT)	823:856	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	6	theme	carbon	833:838	arg1	oxide					866:870	copper oxide	859:870	copper oxide (Cu2O)	859:877	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	6	theme	carbon	833:838	arg1	material					920:927	the target material	909:927	the target material	909:927	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	6	theme	carbon	833:838	arg1	α-CNT					851:855	α-CNT	851:855	α-CNT	851:855	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	2	7	theme	plasmon	519:525	arg1	resonance					527:535	surface plasmon resonance	511:535	the surface plasmon resonance (SPR) characteristics peak	507:562	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	2	7	theme	plasmon	519:525	arg1	SPR					538:540	SPR	538:540	SPR	538:540	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	6	8	theme	capped	1048:1053	arg1	AuNPs					1055:1059	the chitosan capped AuNPs	1035:1059	the chitosan capped AuNPs	1035:1059	The absorption spectra showed dramatic intensity increased and red shifted once the analyte was added to the chitosan capped AuNPs.
25215315	5	9	theme	uptake	783:788	arg1	analyte					790:796	uptake analyte	783:796	uptake analyte	783:796	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	6	10	dep	showed	953:958	arg1	increased					979:987	increased	979:987	showed dramatic intensity increased	953:987	The absorption spectra showed dramatic intensity increased and red shifted once the analyte was added to the chitosan capped AuNPs.
25215315	0	11	theme	gold	74:77	arg1	nanoparticles					79:91	gold nanoparticles	74:91	gold nanoparticles	74:91	Synthesis and ultraviolet visible spectroscopy studies of chitosan capped gold nanoparticles and their reactions with analytes.
25215315	3	12	theme	stable	574:579	arg1	AuNPs					581:585	The most stable AuNPs	565:585	The most stable AuNPs	565:585	The most stable AuNPs occurred for composition with the lowest (1 g) weight of chitosan.
25215315	1	13	theme	various	181:187	arg1	molarities					189:198	various molarities	181:198	various molarities	181:198	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	0	14	theme	Synthesis	0:8	arg1	studies					47:53	Synthesis and ultraviolet visible spectroscopy studies	0:53	Synthesis and ultraviolet visible spectroscopy studies of chitosan	0:65	Synthesis and ultraviolet visible spectroscopy studies of chitosan capped gold nanoparticles and their reactions with analytes.
25215315	0	15	with	reactions	103:111	arg1	analytes					118:125	analytes	118:125	analytes	118:125	Synthesis and ultraviolet visible spectroscopy studies of chitosan capped gold nanoparticles and their reactions with analytes.
25215315	5	16	theme	zinc	884:887	arg1	sulphate					889:896	zinc sulphate	884:896	zinc sulphate (ZnSO4)	884:904	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	16	theme	zinc	884:887	arg1	nanotubes					840:848	amorphous carbon nanotubes	823:848	amorphous carbon nanotubes (α-CNT)	823:856	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	16	theme	zinc	884:887	arg1	oxide					866:870	copper oxide	859:870	copper oxide (Cu2O)	859:877	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	16	theme	zinc	884:887	arg1	material					920:927	the target material	909:927	the target material	909:927	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	16	theme	zinc	884:887	arg1	ZnSO4					899:903	ZnSO4	899:903	ZnSO4	899:903	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	2	17	theme	electron	380:387	arg1	TEM					401:403	TEM	401:403	TEM	401:403	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	2	17	theme	electron	380:387	arg1	microscopy					389:398	transmission electron microscopy	367:398	transmission electron microscopy (TEM) images	367:411	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	0	18	theme	visible	26:32	arg1	spectroscopy					34:45	ultraviolet visible spectroscopy	14:45	ultraviolet visible spectroscopy	14:45	Synthesis and ultraviolet visible spectroscopy studies of chitosan capped gold nanoparticles and their reactions with analytes.
25215315	1	19	theme	stabilizer	263:272	arg1	chitosan					274:281	stabilizer chitosan	263:281	stabilizer chitosan	263:281	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	4	20	theme	bare	731:734	arg1	AuNPs					736:740	bare AuNPs	731:740	bare AuNPs	731:740	AuNPs capped with chitosan size stayed small after 1 month aging compared to bare AuNPs.
25215315	2	21	theme	UV-Vis	417:422	arg1	spectra					435:441	UV-Vis absorption spectra	417:441	UV-Vis absorption spectra	417:441	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	2	22	theme	absorption	424:433	arg1	spectra					435:441	UV-Vis absorption spectra	417:441	UV-Vis absorption spectra	417:441	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	1	23	theme	chitosan	274:281	arg1	weights					204:210	weights	204:210	weights	204:210	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	1	23	theme	chitosan	274:281	arg1	molarities					189:198	various molarities	181:198	various molarities	181:198	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	4	24	theme	chitosan	672:679	arg1	size					681:684	chitosan size	672:684	chitosan size	672:684	AuNPs capped with chitosan size stayed small after 1 month aging compared to bare AuNPs.
25215315	2	25	theme	characteristics	543:557	arg1	peak					559:562	the surface plasmon resonance (SPR) characteristics peak	507:562	the surface plasmon resonance (SPR) characteristics peak	507:562	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	0	26	theme	spectroscopy	34:45	arg1	studies					47:53	Synthesis and ultraviolet visible spectroscopy studies	0:53	Synthesis and ultraviolet visible spectroscopy studies of chitosan	0:65	Synthesis and ultraviolet visible spectroscopy studies of chitosan capped gold nanoparticles and their reactions with analytes.
25215315	5	27	theme	chitosan	758:765	arg1	AuNPs					774:778	chitosan capped AuNPs	758:778	chitosan capped AuNPs	758:778	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	1	28	theme	reducing	215:222	arg1	agent					224:228	reducing agent	215:228	reducing agent	215:228	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	2	29	theme	interparticles	456:469	arg1	distance					471:478	the interparticles distance	452:478	the interparticles distance	452:478	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	5	30	theme	AuNPs	774:778	arg1	ability					747:753	The ability	743:753	The ability of chitosan capped AuNPs to uptake analyte	743:796	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	1	31	theme	Gold	128:131	arg1	nanoparticles					133:145	Gold nanoparticles	128:145	Gold nanoparticles (AuNPs)	128:153	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	1	31	theme	Gold	128:131	arg1	AuNPs					148:152	AuNPs	148:152	AuNPs	148:152	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	3	32	theme	lowest	621:626	arg1	weight					634:639	the lowest (1 g) weight	617:639	the lowest (1 g) weight of chitosan	617:651	The most stable AuNPs occurred for composition with the lowest (1 g) weight of chitosan.
25215315	3	32	theme	lowest	621:626	arg1	g					631:631	1 g	629:631	1 g	629:631	The most stable AuNPs occurred for composition with the lowest (1 g) weight of chitosan.
25215315	5	33	theme	target	913:918	arg1	sulphate					889:896	zinc sulphate	884:896	zinc sulphate (ZnSO4)	884:904	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	33	theme	target	913:918	arg1	nanotubes					840:848	amorphous carbon nanotubes	823:848	amorphous carbon nanotubes (α-CNT)	823:856	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	33	theme	target	913:918	arg1	oxide					866:870	copper oxide	859:870	copper oxide (Cu2O)	859:877	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	33	theme	target	913:918	arg1	material					920:927	the target material	909:927	the target material	909:927	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	2	34	theme	chitosan	318:325	arg1	significance					302:313	The significance	298:313	The significance of chitosan as stabilizer	298:339	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	1	35	theme	agent	224:228	arg1	weights					204:210	weights	204:210	weights	204:210	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	1	35	theme	agent	224:228	arg1	molarities					189:198	various molarities	181:198	various molarities	181:198	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	3	36	theme	chitosan	644:651	arg1	weight					634:639	the lowest (1 g) weight	617:639	the lowest (1 g) weight of chitosan	617:651	The most stable AuNPs occurred for composition with the lowest (1 g) weight of chitosan.
25215315	3	36	theme	chitosan	644:651	arg1	g					631:631	1 g	629:631	1 g	629:631	The most stable AuNPs occurred for composition with the lowest (1 g) weight of chitosan.
25215315	6	37	theme	chitosan	1039:1046	arg1	AuNPs					1055:1059	the chitosan capped AuNPs	1035:1059	the chitosan capped AuNPs	1035:1059	The absorption spectra showed dramatic intensity increased and red shifted once the analyte was added to the chitosan capped AuNPs.
25215315	4	38	theme	month	707:711	arg1	aging					713:717	1 month aging	705:717	1 month aging	705:717	AuNPs capped with chitosan size stayed small after 1 month aging compared to bare AuNPs.
25215315	2	39	theme	surface	511:517	arg1	resonance					527:535	surface plasmon resonance	511:535	the surface plasmon resonance (SPR) characteristics peak	507:562	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	2	39	theme	surface	511:517	arg1	SPR					538:540	SPR	538:540	SPR	538:540	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	5	40	theme	amorphous	823:831	arg1	sulphate					889:896	zinc sulphate	884:896	zinc sulphate (ZnSO4)	884:904	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	40	theme	amorphous	823:831	arg1	nanotubes					840:848	amorphous carbon nanotubes	823:848	amorphous carbon nanotubes (α-CNT)	823:856	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	40	theme	amorphous	823:831	arg1	oxide					866:870	copper oxide	859:870	copper oxide (Cu2O)	859:877	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	40	theme	amorphous	823:831	arg1	material					920:927	the target material	909:927	the target material	909:927	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	5	40	theme	amorphous	823:831	arg1	α-CNT					851:855	α-CNT	851:855	α-CNT	851:855	The ability of chitosan capped AuNPs to uptake analyte was studied by employing amorphous carbon nanotubes (α-CNT), copper oxide (Cu2O), and zinc sulphate (ZnSO4) as the target material.
25215315	2	41	theme	transmission	367:378	arg1	TEM					401:403	TEM	401:403	TEM	401:403	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	2	41	theme	transmission	367:378	arg1	microscopy					389:398	transmission electron microscopy	367:398	transmission electron microscopy (TEM) images	367:411	The significance of chitosan as stabilizer was distinguished through transmission electron microscopy (TEM) images and UV-Vis absorption spectra in which the interparticles distance increases whilst retaining the surface plasmon resonance (SPR) characteristics peak.
25215315	1	42	theme	monosodium	231:240	arg1	MSG					253:255	MSG	253:255	MSG	253:255	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	1	42	theme	monosodium	231:240	arg1	glutamate					242:250	monosodium glutamate	231:250	monosodium glutamate (MSG)	231:256	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	0	43	theme	chitosan	58:65	arg1	studies					47:53	Synthesis and ultraviolet visible spectroscopy studies	0:53	Synthesis and ultraviolet visible spectroscopy studies of chitosan	0:65	Synthesis and ultraviolet visible spectroscopy studies of chitosan capped gold nanoparticles and their reactions with analytes.
25215315	0	44	theme	ultraviolet	14:24	arg1	spectroscopy					34:45	ultraviolet visible spectroscopy	14:45	ultraviolet visible spectroscopy	14:45	Synthesis and ultraviolet visible spectroscopy studies of chitosan capped gold nanoparticles and their reactions with analytes.
25215315	6	45	theme	dramatic	960:967	arg1	intensity					969:977	dramatic intensity	960:977	dramatic intensity	960:977	The absorption spectra showed dramatic intensity increased and red shifted once the analyte was added to the chitosan capped AuNPs.
25215315	1	46	theme	glutamate	242:250	arg1	weights					204:210	weights	204:210	weights	204:210	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
25215315	1	46	theme	glutamate	242:250	arg1	molarities					189:198	various molarities	181:198	various molarities	181:198	Gold nanoparticles (AuNPs) had been synthesized with various molarities and weights of reducing agent, monosodium glutamate (MSG), and stabilizer chitosan, respectively.
28533304	8	0	from	inflammation	1333:1344	arg1	model					1370:1374	a mouse model	1362:1374	a mouse model of NAFLD	1362:1383	In conclusion, our data indicate a causal link between changes in gut microbiota and hepatic inflammation and fibrosis in a mouse model of NAFLD, possibly via alterations in bile acids.
28533304	4	1	with	concurrent	743:752	arg1	levels					806:811	markedly elevated plasma and hepatic bile acid levels	759:811	markedly elevated plasma and hepatic bile acid levels	759:811	Strikingly, despite reducing adipose tissue mass and inflammation, GG enhanced hepatic inflammation and fibrosis, concurrent with markedly elevated plasma and hepatic bile acid levels.
28533304	8	2	from	link	1282:1285	arg1	model					1370:1374	a mouse model	1362:1374	a mouse model of NAFLD	1362:1383	In conclusion, our data indicate a causal link between changes in gut microbiota and hepatic inflammation and fibrosis in a mouse model of NAFLD, possibly via alterations in bile acids.
28533304	2	3	theme	bacteria	295:302	arg1	role					279:282	the role	275:282	the role of the gut bacteria in NAFLD	275:311	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	1	4	theme	NAFLD	217:221	arg1	pathogenesis					201:212	the pathogenesis	197:212	the pathogenesis of NAFLD	197:221	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	8	5	theme	bile	1414:1417	arg1	acids					1419:1423	bile acids	1414:1423	bile acids	1414:1423	In conclusion, our data indicate a causal link between changes in gut microbiota and hepatic inflammation and fibrosis in a mouse model of NAFLD, possibly via alterations in bile acids.
28533304	2	6	from	role	279:282	arg1	NAFLD					307:311	NAFLD	307:311	NAFLD	307:311	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	6	7	theme	portal	1083:1088	arg1	levels					1110:1115	portal secondary bile acid levels	1083:1115	portal secondary bile acid levels	1083:1115	In contrast to GG, chronic oral administration of antibiotics effectively suppressed the gut bacteria, decreased portal secondary bile acid levels, and attenuated hepatic inflammation and fibrosis.
28533304	0	8	theme	potential	77:85	arg1	role					87:90	a potential role	75:90	a potential role for bile acids	75:105	Modulation of the gut microbiota impacts nonalcoholic fatty liver disease: a potential role for bile acids.
28533304	5	9	theme	mice	897:900	arg1	treatment					884:892	treatment	884:892	treatment of mice with taurocholic acid	884:922	Consistent with a role of elevated bile acids in the liver phenotype, treatment of mice with taurocholic acid stimulated hepatic inflammation and fibrosis.
28533304	4	10	theme	adipose	658:664	arg1	mass					673:676	adipose tissue mass	658:676	adipose tissue mass	658:676	Strikingly, despite reducing adipose tissue mass and inflammation, GG enhanced hepatic inflammation and fibrosis, concurrent with markedly elevated plasma and hepatic bile acid levels.
28533304	2	11	theme	gut	428:430	arg1	bacteria					432:439	the gut bacteria	424:439	the gut bacteria	424:439	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	5	12	theme	bile	849:852	arg1	acids					854:858	elevated bile acids	840:858	elevated bile acids	840:858	Consistent with a role of elevated bile acids in the liver phenotype, treatment of mice with taurocholic acid stimulated hepatic inflammation and fibrosis.
28533304	4	13	theme	hepatic	788:794	arg1	acid					801:804	hepatic bile acid	788:804	hepatic bile acid	788:804	Strikingly, despite reducing adipose tissue mass and inflammation, GG enhanced hepatic inflammation and fibrosis, concurrent with markedly elevated plasma and hepatic bile acid levels.
28533304	5	14	from	role	832:835	arg1	phenotype					873:881	the liver phenotype	863:881	the liver phenotype	863:881	Consistent with a role of elevated bile acids in the liver phenotype, treatment of mice with taurocholic acid stimulated hepatic inflammation and fibrosis.
28533304	2	15	theme	guar	393:396	arg1	fiber					386:390	the fermentable dietary fiber	362:390	the fermentable dietary fiber	362:390	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	2	15	theme	guar	393:396	arg1	gum					398:400	guar gum	393:400	guar gum (GG)	393:405	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	2	15	theme	guar	393:396	arg1	GG					403:404	GG	403:404	GG	403:404	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	8	16	theme	causal	1275:1280	arg1	link					1282:1285	a causal link	1273:1285	a causal link between changes in gut microbiota	1273:1319	In conclusion, our data indicate a causal link between changes in gut microbiota and hepatic inflammation and fibrosis in a mouse model of NAFLD, possibly via alterations in bile acids.
28533304	8	17	theme	gut	1306:1308	arg1	microbiota					1310:1319	gut microbiota	1306:1319	gut microbiota	1306:1319	In conclusion, our data indicate a causal link between changes in gut microbiota and hepatic inflammation and fibrosis in a mouse model of NAFLD, possibly via alterations in bile acids.
28533304	6	18	theme	secondary	1090:1098	arg1	levels					1110:1115	portal secondary bile acid levels	1083:1115	portal secondary bile acid levels	1083:1115	In contrast to GG, chronic oral administration of antibiotics effectively suppressed the gut bacteria, decreased portal secondary bile acid levels, and attenuated hepatic inflammation and fibrosis.
28533304	4	19	theme	hepatic	708:714	arg1	inflammation					716:727	hepatic inflammation	708:727	hepatic inflammation	708:727	Strikingly, despite reducing adipose tissue mass and inflammation, GG enhanced hepatic inflammation and fibrosis, concurrent with markedly elevated plasma and hepatic bile acid levels.
28533304	6	20	theme	antibiotics	1020:1030	arg1	administration					1002:1015	chronic oral administration	989:1015	chronic oral administration of antibiotics	989:1030	In contrast to GG, chronic oral administration of antibiotics effectively suppressed the gut bacteria, decreased portal secondary bile acid levels, and attenuated hepatic inflammation and fibrosis.
28533304	2	21	theme	gut	332:334	arg1	bacteria					336:343	the gut bacteria	328:343	the gut bacteria	328:343	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	6	22	theme	acid	1105:1108	arg1	levels					1110:1115	portal secondary bile acid levels	1083:1115	portal secondary bile acid levels	1083:1115	In contrast to GG, chronic oral administration of antibiotics effectively suppressed the gut bacteria, decreased portal secondary bile acid levels, and attenuated hepatic inflammation and fibrosis.
28533304	4	23	theme	acid	801:804	arg1	levels					806:811	markedly elevated plasma and hepatic bile acid levels	759:811	markedly elevated plasma and hepatic bile acid levels	759:811	Strikingly, despite reducing adipose tissue mass and inflammation, GG enhanced hepatic inflammation and fibrosis, concurrent with markedly elevated plasma and hepatic bile acid levels.
28533304	7	24	theme	plasma	1206:1211	arg1	levels					1232:1237	plasma lipopolysaccharide levels	1206:1237	plasma lipopolysaccharide levels	1206:1237	Neither GG nor antibiotics influenced plasma lipopolysaccharide levels.
28533304	3	25	theme	GG	489:490	arg1	feeding					492:498	GG feeding	489:498	GG feeding	489:498	GG feeding profoundly altered the gut microbiota composition, in parallel with reduced diet-induced obesity and improved glucose tolerance.
28533304	8	26	from	fibrosis	1350:1357	arg1	model					1370:1374	a mouse model	1362:1374	a mouse model of NAFLD	1362:1383	In conclusion, our data indicate a causal link between changes in gut microbiota and hepatic inflammation and fibrosis in a mouse model of NAFLD, possibly via alterations in bile acids.
28533304	6	27	theme	oral	997:1000	arg1	administration					1002:1015	chronic oral administration	989:1015	chronic oral administration of antibiotics	989:1030	In contrast to GG, chronic oral administration of antibiotics effectively suppressed the gut bacteria, decreased portal secondary bile acid levels, and attenuated hepatic inflammation and fibrosis.
28533304	5	28	with	treatment	884:892	arg1	acid					919:922	taurocholic acid	907:922	taurocholic acid	907:922	Consistent with a role of elevated bile acids in the liver phenotype, treatment of mice with taurocholic acid stimulated hepatic inflammation and fibrosis.
28533304	4	29	theme	bile	796:799	arg1	acid					801:804	hepatic bile acid	788:804	hepatic bile acid	788:804	Strikingly, despite reducing adipose tissue mass and inflammation, GG enhanced hepatic inflammation and fibrosis, concurrent with markedly elevated plasma and hepatic bile acid levels.
28533304	6	30	theme	bile	1100:1103	arg1	levels					1110:1115	portal secondary bile acid levels	1083:1115	portal secondary bile acid levels	1083:1115	In contrast to GG, chronic oral administration of antibiotics effectively suppressed the gut bacteria, decreased portal secondary bile acid levels, and attenuated hepatic inflammation and fibrosis.
28533304	3	31	with	parallel	554:561	arg1	tolerance					618:626	improved glucose tolerance	601:626	improved glucose tolerance	601:626	GG feeding profoundly altered the gut microbiota composition, in parallel with reduced diet-induced obesity and improved glucose tolerance.
28533304	3	31	with	parallel	554:561	arg1	obesity					589:595	reduced diet-induced obesity	568:595	reduced diet-induced obesity	568:595	GG feeding profoundly altered the gut microbiota composition, in parallel with reduced diet-induced obesity and improved glucose tolerance.
28533304	6	32	theme	chronic	989:995	arg1	administration					1002:1015	chronic oral administration	989:1015	chronic oral administration of antibiotics	989:1030	In contrast to GG, chronic oral administration of antibiotics effectively suppressed the gut bacteria, decreased portal secondary bile acid levels, and attenuated hepatic inflammation and fibrosis.
28533304	0	33	theme	gut	18:20	arg1	microbiota					22:31	the gut microbiota	14:31	the gut microbiota	14:31	Modulation of the gut microbiota impacts nonalcoholic fatty liver disease: a potential role for bile acids.
28533304	5	34	theme	hepatic	935:941	arg1	inflammation					943:954	hepatic inflammation	935:954	hepatic inflammation	935:954	Consistent with a role of elevated bile acids in the liver phenotype, treatment of mice with taurocholic acid stimulated hepatic inflammation and fibrosis.
28533304	4	35	theme	elevated	768:775	arg1	levels					806:811	markedly elevated plasma and hepatic bile acid levels	759:811	markedly elevated plasma and hepatic bile acid levels	759:811	Strikingly, despite reducing adipose tissue mass and inflammation, GG enhanced hepatic inflammation and fibrosis, concurrent with markedly elevated plasma and hepatic bile acid levels.
28533304	4	36	theme	plasma	777:782	arg1	levels					806:811	markedly elevated plasma and hepatic bile acid levels	759:811	markedly elevated plasma and hepatic bile acid levels	759:811	Strikingly, despite reducing adipose tissue mass and inflammation, GG enhanced hepatic inflammation and fibrosis, concurrent with markedly elevated plasma and hepatic bile acid levels.
28533304	1	37	theme	common	161:166	arg1	disease					133:139	Nonalcoholic fatty liver disease	108:139	Nonalcoholic fatty liver disease (NAFLD)	108:147	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	1	37	theme	common	161:166	arg1	worldwide					182:190	the most common liver disease worldwide	152:190	the most common liver disease worldwide	152:190	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	2	38	theme	fermentable	366:376	arg1	fiber					386:390	the fermentable dietary fiber	362:390	the fermentable dietary fiber	362:390	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	2	38	theme	fermentable	366:376	arg1	gum					398:400	guar gum	393:400	guar gum (GG)	393:405	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	3	39	theme	reduced	568:574	arg1	obesity					589:595	reduced diet-induced obesity	568:595	reduced diet-induced obesity	568:595	GG feeding profoundly altered the gut microbiota composition, in parallel with reduced diet-induced obesity and improved glucose tolerance.
28533304	5	40	theme	elevated	840:847	arg1	acids					854:858	elevated bile acids	840:858	elevated bile acids	840:858	Consistent with a role of elevated bile acids in the liver phenotype, treatment of mice with taurocholic acid stimulated hepatic inflammation and fibrosis.
28533304	8	41	theme	hepatic	1325:1331	arg1	inflammation					1333:1344	hepatic inflammation	1325:1344	hepatic inflammation	1325:1344	In conclusion, our data indicate a causal link between changes in gut microbiota and hepatic inflammation and fibrosis in a mouse model of NAFLD, possibly via alterations in bile acids.
28533304	1	42	theme	liver	168:172	arg1	disease					133:139	Nonalcoholic fatty liver disease	108:139	Nonalcoholic fatty liver disease (NAFLD)	108:147	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	1	42	theme	liver	168:172	arg1	worldwide					182:190	the most common liver disease worldwide	152:190	the most common liver disease worldwide	152:190	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	2	43	theme	oral	453:456	arg1	administration					458:471	chronic oral administration	445:471	chronic oral administration of antibiotics	445:486	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	3	44	theme	diet-induced	576:587	arg1	obesity					589:595	reduced diet-induced obesity	568:595	reduced diet-induced obesity	568:595	GG feeding profoundly altered the gut microbiota composition, in parallel with reduced diet-induced obesity and improved glucose tolerance.
28533304	0	45	theme	bile	96:99	arg1	acids					101:105	bile acids	96:105	bile acids	96:105	Modulation of the gut microbiota impacts nonalcoholic fatty liver disease: a potential role for bile acids.
28533304	1	46	theme	disease	174:180	arg1	disease					133:139	Nonalcoholic fatty liver disease	108:139	Nonalcoholic fatty liver disease (NAFLD)	108:147	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	1	46	theme	disease	174:180	arg1	worldwide					182:190	the most common liver disease worldwide	152:190	the most common liver disease worldwide	152:190	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	0	47	theme	microbiota	22:31	arg1	Modulation					0:9	Modulation	0:9	Modulation of the gut microbiota	0:31	Modulation of the gut microbiota impacts nonalcoholic fatty liver disease: a potential role for bile acids.
28533304	2	48	theme	chronic	445:451	arg1	administration					458:471	chronic oral administration	445:471	chronic oral administration of antibiotics	445:486	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	2	49	theme	dietary	378:384	arg1	fiber					386:390	the fermentable dietary fiber	362:390	the fermentable dietary fiber	362:390	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	2	49	theme	dietary	378:384	arg1	gum					398:400	guar gum	393:400	guar gum (GG)	393:405	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	0	50	theme	fatty	54:58	arg1	disease					66:72	nonalcoholic fatty liver disease	41:72	nonalcoholic fatty liver disease	41:72	Modulation of the gut microbiota impacts nonalcoholic fatty liver disease: a potential role for bile acids.
28533304	5	51	with	Consistent	814:823	arg1	role					832:835	a role	830:835	a role of elevated bile acids in the liver phenotype	830:881	Consistent with a role of elevated bile acids in the liver phenotype, treatment of mice with taurocholic acid stimulated hepatic inflammation and fibrosis.
28533304	8	52	from	alterations	1399:1409	arg1	acids					1419:1423	bile acids	1414:1423	bile acids	1414:1423	In conclusion, our data indicate a causal link between changes in gut microbiota and hepatic inflammation and fibrosis in a mouse model of NAFLD, possibly via alterations in bile acids.
28533304	0	53	dep	impacts	33:39	arg1	role					87:90	a potential role	75:90	a potential role for bile acids	75:105	Modulation of the gut microbiota impacts nonalcoholic fatty liver disease: a potential role for bile acids.
28533304	2	54	theme	gut	291:293	arg1	bacteria					295:302	the gut bacteria	287:302	the gut bacteria	287:302	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	0	55	theme	nonalcoholic	41:52	arg1	disease					66:72	nonalcoholic fatty liver disease	41:72	nonalcoholic fatty liver disease	41:72	Modulation of the gut microbiota impacts nonalcoholic fatty liver disease: a potential role for bile acids.
28533304	8	56	theme	mouse	1364:1368	arg1	model					1370:1374	a mouse model	1362:1374	a mouse model of NAFLD	1362:1383	In conclusion, our data indicate a causal link between changes in gut microbiota and hepatic inflammation and fibrosis in a mouse model of NAFLD, possibly via alterations in bile acids.
28533304	3	57	theme	improved	601:608	arg1	tolerance					618:626	improved glucose tolerance	601:626	improved glucose tolerance	601:626	GG feeding profoundly altered the gut microbiota composition, in parallel with reduced diet-induced obesity and improved glucose tolerance.
28533304	2	58	dep	mice	357:360	arg1	fiber					386:390	the fermentable dietary fiber	362:390	the fermentable dietary fiber	362:390	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	2	58	dep	mice	357:360	arg1	gum					398:400	guar gum	393:400	guar gum (GG)	393:405	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	5	59	theme	acids	854:858	arg1	role					832:835	a role	830:835	a role of elevated bile acids in the liver phenotype	830:881	Consistent with a role of elevated bile acids in the liver phenotype, treatment of mice with taurocholic acid stimulated hepatic inflammation and fibrosis.
28533304	7	60	theme	lipopolysaccharide	1213:1230	arg1	levels					1232:1237	plasma lipopolysaccharide levels	1206:1237	plasma lipopolysaccharide levels	1206:1237	Neither GG nor antibiotics influenced plasma lipopolysaccharide levels.
28533304	5	61	theme	liver	867:871	arg1	phenotype					873:881	the liver phenotype	863:881	the liver phenotype	863:881	Consistent with a role of elevated bile acids in the liver phenotype, treatment of mice with taurocholic acid stimulated hepatic inflammation and fibrosis.
28533304	3	62	theme	glucose	610:616	arg1	tolerance					618:626	improved glucose tolerance	601:626	improved glucose tolerance	601:626	GG feeding profoundly altered the gut microbiota composition, in parallel with reduced diet-induced obesity and improved glucose tolerance.
28533304	3	63	theme	gut	523:525	arg1	composition					538:548	the gut microbiota composition	519:548	the gut microbiota composition	519:548	GG feeding profoundly altered the gut microbiota composition, in parallel with reduced diet-induced obesity and improved glucose tolerance.
28533304	1	64	theme	Nonalcoholic	108:119	arg1	disease					133:139	Nonalcoholic fatty liver disease	108:139	Nonalcoholic fatty liver disease (NAFLD)	108:147	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	1	64	theme	Nonalcoholic	108:119	arg1	worldwide					182:190	the most common liver disease worldwide	152:190	the most common liver disease worldwide	152:190	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	1	64	theme	Nonalcoholic	108:119	arg1	NAFLD					142:146	NAFLD	142:146	NAFLD	142:146	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	6	65	theme	gut	1059:1061	arg1	bacteria					1063:1070	the gut bacteria	1055:1070	the gut bacteria	1055:1070	In contrast to GG, chronic oral administration of antibiotics effectively suppressed the gut bacteria, decreased portal secondary bile acid levels, and attenuated hepatic inflammation and fibrosis.
28533304	2	66	theme	antibiotics	476:486	arg1	administration					458:471	chronic oral administration	445:471	chronic oral administration of antibiotics	445:486	Here, we investigated the role of the gut bacteria in NAFLD by stimulating the gut bacteria via feeding mice the fermentable dietary fiber, guar gum (GG), and suppressing the gut bacteria via chronic oral administration of antibiotics.
28533304	8	67	theme	NAFLD	1379:1383	arg1	model					1370:1374	a mouse model	1362:1374	a mouse model of NAFLD	1362:1383	In conclusion, our data indicate a causal link between changes in gut microbiota and hepatic inflammation and fibrosis in a mouse model of NAFLD, possibly via alterations in bile acids.
28533304	1	68	theme	fatty	121:125	arg1	disease					133:139	Nonalcoholic fatty liver disease	108:139	Nonalcoholic fatty liver disease (NAFLD)	108:147	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	1	68	theme	fatty	121:125	arg1	worldwide					182:190	the most common liver disease worldwide	152:190	the most common liver disease worldwide	152:190	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	1	68	theme	fatty	121:125	arg1	NAFLD					142:146	NAFLD	142:146	NAFLD	142:146	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	4	69	theme	tissue	666:671	arg1	mass					673:676	adipose tissue mass	658:676	adipose tissue mass	658:676	Strikingly, despite reducing adipose tissue mass and inflammation, GG enhanced hepatic inflammation and fibrosis, concurrent with markedly elevated plasma and hepatic bile acid levels.
28533304	3	70	theme	microbiota	527:536	arg1	composition					538:548	the gut microbiota composition	519:548	the gut microbiota composition	519:548	GG feeding profoundly altered the gut microbiota composition, in parallel with reduced diet-induced obesity and improved glucose tolerance.
28533304	1	71	theme	liver	127:131	arg1	disease					133:139	Nonalcoholic fatty liver disease	108:139	Nonalcoholic fatty liver disease (NAFLD)	108:147	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	1	71	theme	liver	127:131	arg1	worldwide					182:190	the most common liver disease worldwide	152:190	the most common liver disease worldwide	152:190	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	1	71	theme	liver	127:131	arg1	NAFLD					142:146	NAFLD	142:146	NAFLD	142:146	Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, yet the pathogenesis of NAFLD is only partially understood.
28533304	8	72	from	changes	1295:1301	arg1	microbiota					1310:1319	gut microbiota	1306:1319	gut microbiota	1306:1319	In conclusion, our data indicate a causal link between changes in gut microbiota and hepatic inflammation and fibrosis in a mouse model of NAFLD, possibly via alterations in bile acids.
28533304	0	73	theme	liver	60:64	arg1	disease					66:72	nonalcoholic fatty liver disease	41:72	nonalcoholic fatty liver disease	41:72	Modulation of the gut microbiota impacts nonalcoholic fatty liver disease: a potential role for bile acids.
28533304	6	74	theme	hepatic	1133:1139	arg1	inflammation					1141:1152	hepatic inflammation	1133:1152	hepatic inflammation	1133:1152	In contrast to GG, chronic oral administration of antibiotics effectively suppressed the gut bacteria, decreased portal secondary bile acid levels, and attenuated hepatic inflammation and fibrosis.
28533304	5	75	theme	taurocholic	907:917	arg1	acid					919:922	taurocholic acid	907:922	taurocholic acid	907:922	Consistent with a role of elevated bile acids in the liver phenotype, treatment of mice with taurocholic acid stimulated hepatic inflammation and fibrosis.
28159280	4	0	dep	range	525:529	arg1	e.g.					518:521	e.g.	518:521	e.g.	518:521	Nuclear magnetic resonance spectroscopy revealed 33 components in eel, e.g. a range of organic acids, amino acids, alcohols, and sugars.
28159280	6	1	theme	flavour	996:1002	arg1	formation					1004:1012	the flavour formation	992:1012	the flavour formation of vinasse eel	992:1027	The abundant sucrose, glutamate, creatine, and lactate could take an active part in the flavour formation of vinasse eel.
28159280	4	2	theme	amino	549:553	arg1	acids					555:559	amino acids	549:559	amino acids	549:559	Nuclear magnetic resonance spectroscopy revealed 33 components in eel, e.g. a range of organic acids, amino acids, alcohols, and sugars.
28159280	4	3	from	components	499:508	arg1	eel					513:515	eel	513:515	eel	513:515	Nuclear magnetic resonance spectroscopy revealed 33 components in eel, e.g. a range of organic acids, amino acids, alcohols, and sugars.
28159280	1	4	theme	traditional	141:151	arg1	eel					110:112	Vinasse pike eel	97:112	Vinasse pike eel (Muraenesox cinereus)	97:134	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	4	theme	traditional	141:151	arg1	food					161:164	a traditional Chinese food	139:164	a traditional Chinese food with a characteristic flavour, taste, and nutritional composition	139:230	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	2	5	theme	molecular	271:279	arg1	composition					293:303	the molecular nutritional composition	267:303	the molecular nutritional composition of this food pickled product	267:332	Its flavour is closely related to the molecular nutritional composition of this food pickled product.
28159280	6	6	theme	abundant	912:919	arg1	sucrose					921:927	abundant sucrose	912:927	abundant sucrose	912:927	The abundant sucrose, glutamate, creatine, and lactate could take an active part in the flavour formation of vinasse eel.
28159280	7	7	theme	eel	1105:1107	arg1	characteristics					1078:1092	the nutritional characteristics	1062:1092	the nutritional characteristics of vinasse eel	1062:1107	This work provides insight into the nutritional characteristics of vinasse eel during pickling.
28159280	4	8	theme	magnetic	455:462	arg1	resonance					464:472	Nuclear magnetic resonance	447:472	Nuclear magnetic resonance spectroscopy	447:485	Nuclear magnetic resonance spectroscopy revealed 33 components in eel, e.g. a range of organic acids, amino acids, alcohols, and sugars.
28159280	5	9	theme	amino	895:899	arg1	acids					901:905	some amino acids	890:905	some amino acids	890:905	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	4	10	theme	acids	555:559	arg1	range					525:529	a range	523:529	a range of organic acids, amino acids, alcohols, and sugars	523:581	Nuclear magnetic resonance spectroscopy revealed 33 components in eel, e.g. a range of organic acids, amino acids, alcohols, and sugars.
28159280	7	11	theme	vinasse	1097:1103	arg1	eel					1105:1107	vinasse eel	1097:1107	vinasse eel	1097:1107	This work provides insight into the nutritional characteristics of vinasse eel during pickling.
28159280	4	12	theme	Nuclear	447:453	arg1	resonance					464:472	Nuclear magnetic resonance	447:472	Nuclear magnetic resonance spectroscopy	447:485	Nuclear magnetic resonance spectroscopy revealed 33 components in eel, e.g. a range of organic acids, amino acids, alcohols, and sugars.
28159280	5	13	theme	acids	901:905	arg1	fluctuation					875:885	the fluctuation	871:885	the fluctuation of some amino acids	871:905	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	13	theme	acids	901:905	arg1	creatinine					845:854	creatinine	845:854	creatinine	845:854	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	13	theme	acids	901:905	arg1	creatine					766:773	creatine	766:773	creatine	766:773	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	13	theme	acids	901:905	arg1	accumulation					780:791	the accumulation	776:791	the accumulation of a range of organic acids, alcohols, glucose	776:838	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	13	theme	acids	901:905	arg1	sucrose					754:760	sucrose	754:760	sucrose	754:760	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	1	14	theme	Chinese	153:159	arg1	eel					110:112	Vinasse pike eel	97:112	Vinasse pike eel (Muraenesox cinereus)	97:134	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	14	theme	Chinese	153:159	arg1	food					161:164	a traditional Chinese food	139:164	a traditional Chinese food with a characteristic flavour, taste, and nutritional composition	139:230	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	5	15	theme	creatine	766:773	arg1	consumption					739:749	the consumption	735:749	the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids	735:905	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	16	theme	Multivariate	584:595	arg1	analysis					602:609	Multivariate data analysis	584:609	Multivariate data analysis	584:609	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	4	17	theme	alcohols	562:569	arg1	range					525:529	a range	523:529	a range of organic acids, amino acids, alcohols, and sugars	523:581	Nuclear magnetic resonance spectroscopy revealed 33 components in eel, e.g. a range of organic acids, amino acids, alcohols, and sugars.
28159280	3	18	theme	eel	418:420	arg1	composition					398:408	the nutritional composition	382:408	the nutritional composition of pike eel	382:420	In this study, we characterised the changes in the nutritional composition of pike eel during vinasse pickling.
28159280	0	19	theme	nutritional	10:20	arg1	characteristics					22:36	Molecular nutritional characteristics	0:36	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus)	0:78	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus) during pickling.
28159280	4	20	theme	resonance	464:472	arg1	spectroscopy					474:485	Nuclear magnetic resonance spectroscopy	447:485	Nuclear magnetic resonance spectroscopy	447:485	Nuclear magnetic resonance spectroscopy revealed 33 components in eel, e.g. a range of organic acids, amino acids, alcohols, and sugars.
28159280	5	21	theme	data	597:600	arg1	analysis					602:609	Multivariate data analysis	584:609	Multivariate data analysis	584:609	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	6	22	theme	active	977:982	arg1	part					984:987	an active part	974:987	an active part	974:987	The abundant sucrose, glutamate, creatine, and lactate could take an active part in the flavour formation of vinasse eel.
28159280	0	23	theme	Molecular	0:8	arg1	characteristics					22:36	Molecular nutritional characteristics	0:36	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus)	0:78	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus) during pickling.
28159280	5	24	theme	eel	664:666	arg1	composition					649:659	the nutritional composition	633:659	the nutritional composition of eel	633:666	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	25	theme	acids	815:819	arg1	range					798:802	a range	796:802	a range of organic acids, alcohols, glucose	796:838	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	2	26	theme	nutritional	281:291	arg1	composition					293:303	the molecular nutritional composition	267:303	the molecular nutritional composition of this food pickled product	267:332	Its flavour is closely related to the molecular nutritional composition of this food pickled product.
28159280	1	27	theme	characteristic	173:186	arg1	flavour					188:194	a characteristic flavour	171:194	a characteristic flavour	171:194	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	7	28	theme	nutritional	1066:1076	arg1	characteristics					1078:1092	the nutritional characteristics	1062:1092	the nutritional characteristics of vinasse eel	1062:1107	This work provides insight into the nutritional characteristics of vinasse eel during pickling.
28159280	4	29	theme	acids	542:546	arg1	range					525:529	a range	523:529	a range of organic acids, amino acids, alcohols, and sugars	523:581	Nuclear magnetic resonance spectroscopy revealed 33 components in eel, e.g. a range of organic acids, amino acids, alcohols, and sugars.
28159280	5	30	theme	major	678:682	arg1	changes					684:690	major changes	678:690	major changes	678:690	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	0	31	dep	Muraenesox	59:68	arg1	cinereus					70:77	Muraenesox cinereus	59:77	Muraenesox cinereus	59:77	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus) during pickling.
28159280	5	32	theme	range	798:802	arg1	fluctuation					875:885	the fluctuation	871:885	the fluctuation of some amino acids	871:905	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	32	theme	range	798:802	arg1	creatinine					845:854	creatinine	845:854	creatinine	845:854	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	32	theme	range	798:802	arg1	creatine					766:773	creatine	766:773	creatine	766:773	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	32	theme	range	798:802	arg1	accumulation					780:791	the accumulation	776:791	the accumulation of a range of organic acids, alcohols, glucose	776:838	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	32	theme	range	798:802	arg1	sucrose					754:760	sucrose	754:760	sucrose	754:760	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	0	33	theme	pike	49:52	arg1	Muraenesox					59:68	Muraenesox	59:68	Muraenesox	59:68	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus) during pickling.
28159280	0	33	theme	pike	49:52	arg1	eel					54:56	vinasse pike eel	41:56	vinasse pike eel (Muraenesox cinereus)	41:78	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus) during pickling.
28159280	4	34	theme	organic	534:540	arg1	acids					542:546	organic acids	534:546	organic acids	534:546	Nuclear magnetic resonance spectroscopy revealed 33 components in eel, e.g. a range of organic acids, amino acids, alcohols, and sugars.
28159280	0	35	theme	vinasse	41:47	arg1	Muraenesox					59:68	Muraenesox	59:68	Muraenesox	59:68	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus) during pickling.
28159280	0	35	theme	vinasse	41:47	arg1	eel					54:56	vinasse pike eel	41:56	vinasse pike eel (Muraenesox cinereus)	41:78	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus) during pickling.
28159280	3	36	theme	nutritional	386:396	arg1	composition					398:408	the nutritional composition	382:408	the nutritional composition of pike eel	382:420	In this study, we characterised the changes in the nutritional composition of pike eel during vinasse pickling.
28159280	4	37	theme	sugars	576:581	arg1	range					525:529	a range	523:529	a range of organic acids, amino acids, alcohols, and sugars	523:581	Nuclear magnetic resonance spectroscopy revealed 33 components in eel, e.g. a range of organic acids, amino acids, alcohols, and sugars.
28159280	3	38	theme	vinasse	429:435	arg1	pickling					437:444	vinasse pickling	429:444	vinasse pickling	429:444	In this study, we characterised the changes in the nutritional composition of pike eel during vinasse pickling.
28159280	5	39	theme	nutritional	637:647	arg1	composition					649:659	the nutritional composition	633:659	the nutritional composition of eel	633:666	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	3	40	theme	pike	413:416	arg1	eel					418:420	pike eel	413:420	pike eel	413:420	In this study, we characterised the changes in the nutritional composition of pike eel during vinasse pickling.
28159280	6	41	theme	eel	1025:1027	arg1	formation					1004:1012	the flavour formation	992:1012	the flavour formation of vinasse eel	992:1027	The abundant sucrose, glutamate, creatine, and lactate could take an active part in the flavour formation of vinasse eel.
28159280	2	42	theme	product	326:332	arg1	composition					293:303	the molecular nutritional composition	267:303	the molecular nutritional composition of this food pickled product	267:332	Its flavour is closely related to the molecular nutritional composition of this food pickled product.
28159280	0	43	theme	eel	54:56	arg1	characteristics					22:36	Molecular nutritional characteristics	0:36	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus)	0:78	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus) during pickling.
28159280	2	44	theme	pickled	318:324	arg1	product					326:332	this food pickled product	308:332	this food pickled product	308:332	Its flavour is closely related to the molecular nutritional composition of this food pickled product.
28159280	1	45	theme	pike	105:108	arg1	eel					110:112	Vinasse pike eel	97:112	Vinasse pike eel (Muraenesox cinereus)	97:134	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	45	theme	pike	105:108	arg1	cinereus					126:133	Muraenesox cinereus	115:133	Muraenesox cinereus	115:133	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	45	theme	pike	105:108	arg1	food					161:164	a traditional Chinese food	139:164	a traditional Chinese food with a characteristic flavour, taste, and nutritional composition	139:230	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	46	theme	Muraenesox	115:124	arg1	eel					110:112	Vinasse pike eel	97:112	Vinasse pike eel (Muraenesox cinereus)	97:134	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	46	theme	Muraenesox	115:124	arg1	cinereus					126:133	Muraenesox cinereus	115:133	Muraenesox cinereus	115:133	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	47	theme	Vinasse	97:103	arg1	eel					110:112	Vinasse pike eel	97:112	Vinasse pike eel (Muraenesox cinereus)	97:134	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	47	theme	Vinasse	97:103	arg1	cinereus					126:133	Muraenesox cinereus	115:133	Muraenesox cinereus	115:133	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	47	theme	Vinasse	97:103	arg1	food					161:164	a traditional Chinese food	139:164	a traditional Chinese food with a characteristic flavour, taste, and nutritional composition	139:230	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	48	with	food	161:164	arg1	flavour					188:194	a characteristic flavour	171:194	a characteristic flavour	171:194	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	48	with	food	161:164	arg1	composition					220:230	nutritional composition	208:230	nutritional composition	208:230	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	1	48	with	food	161:164	arg1	taste					197:201	taste	197:201	taste	197:201	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	0	49	dep	characteristics	22:36	arg1	pickling					87:94	pickling	87:94	pickling	87:94	Molecular nutritional characteristics of vinasse pike eel (Muraenesox cinereus) during pickling.
28159280	6	50	theme	vinasse	1017:1023	arg1	eel					1025:1027	vinasse eel	1017:1027	vinasse eel	1017:1027	The abundant sucrose, glutamate, creatine, and lactate could take an active part in the flavour formation of vinasse eel.
28159280	2	51	theme	food	313:316	arg1	product					326:332	this food pickled product	308:332	this food pickled product	308:332	Its flavour is closely related to the molecular nutritional composition of this food pickled product.
28159280	5	52	theme	organic	807:813	arg1	glucose					832:838	glucose	832:838	glucose	832:838	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	52	theme	organic	807:813	arg1	alcohols					822:829	alcohols	822:829	alcohols	822:829	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	5	52	theme	organic	807:813	arg1	acids					815:819	organic acids	807:819	organic acids	807:819	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
28159280	1	53	theme	nutritional	208:218	arg1	composition					220:230	nutritional composition	208:230	nutritional composition	208:230	Vinasse pike eel (Muraenesox cinereus) is a traditional Chinese food with a characteristic flavour, taste, and nutritional composition.
28159280	3	54	from	changes	371:377	arg1	composition					398:408	the nutritional composition	382:408	the nutritional composition of pike eel	382:420	In this study, we characterised the changes in the nutritional composition of pike eel during vinasse pickling.
28159280	5	55	theme	sucrose	754:760	arg1	consumption					739:749	the consumption	735:749	the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids	735:905	Multivariate data analysis further revealed that the nutritional composition of eel undergoes major changes during pickling, which were highlighted by the consumption of sucrose and creatine, the accumulation of a range of organic acids, alcohols, glucose, and creatinine, as well as in the fluctuation of some amino acids.
25370022	12	0	theme	efficient	1741:1749	arg1	manner					1767:1772	an efficient and time-saving manner	1738:1772	an efficient and time-saving manner	1738:1772	PCA was able to differentiate the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner and provided an excellent visualisation of the data.
25370022	2	1	theme	detailed	344:351	arg1	comparison					353:362	a detailed comparison	342:362	a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation	342:488	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	2	1	theme	detailed	344:351	arg1	tedious					498:504	tedious	498:504	tedious	498:504	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	4	2	from	concentrations	849:862	arg1	K4M					769:771	K4M	769:771	K4M	769:771	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	methylcellulose					712:726	hydroxypropyl methylcellulose	698:726	hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations	698:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	LV					791:792	E15 LV	787:792	E15 LV	787:792	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	grades					752:757	ten different grades	738:757	ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations	738:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	LV					812:813	F50 LV	808:813	F50 LV	808:813	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	PEG					684:686	PEG	684:686	PEG	684:686	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	HPMC					729:732	HPMC	729:732	HPMC	729:732	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	F4M					816:818	F4M	816:818	F4M	816:818	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	K100M					780:784	K100M	780:784	K100M	780:784	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	LV					799:800	LV	799:800	LV	799:800	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	VLV					833:835	Methocel VLV	824:835	Methocel VLV	824:835	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	LV					765:766	LV	765:766	LV	765:766	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	E4M					803:805	E4M	803:805	E4M	803:805	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	2	from	concentrations	849:862	arg1	glycol					676:681	polyethylene glycol (PEG) 3350	663:692	polyethylene glycol (PEG) 3350	663:692	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	12	3	theme	different	1696:1704	arg1	formulations					1706:1717	different formulations	1696:1717	different formulations containing HPMC in an efficient and time-saving manner	1696:1772	PCA was able to differentiate the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner and provided an excellent visualisation of the data.
25370022	4	4	from	glycol	676:681	arg1	concentrations					849:862	various concentrations	841:862	various concentrations	841:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	6	5	theme	HPMC	1088:1091	arg1	size					1058:1061	Particle size	1049:1061	Particle size	1049:1061	Particle size and size distribution of HPMC played an important role in the viscosity of melt suspensions.
25370022	6	5	theme	HPMC	1088:1091	arg1	distribution					1072:1083	size distribution	1067:1083	size distribution	1067:1083	Particle size and size distribution of HPMC played an important role in the viscosity of melt suspensions.
25370022	9	6	theme	various	1326:1332	arg1	formulations					1334:1345	the various formulations	1322:1345	the various formulations containing HPMC	1322:1361	The complex viscosity profiles of the various formulations containing HPMC were successfully classified into three clusters of low, moderate and high viscosity.
25370022	12	7	theme	complex	1666:1672	arg1	profiles					1684:1691	the complex viscosity profiles	1662:1691	the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner	1662:1772	PCA was able to differentiate the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner and provided an excellent visualisation of the data.
25370022	1	8	theme	rheological	174:184	arg1	behaviour					186:194	the rheological behaviour	170:194	the rheological behaviour of polymer melt suspensions	170:222	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	4	9	theme	Methocel	824:831	arg1	grades					752:757	ten different grades	738:757	ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations	738:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	9	theme	Methocel	824:831	arg1	VLV					833:835	Methocel VLV	824:835	Methocel VLV	824:835	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	0	10	theme	surface	102:108	arg1	plots					110:114	surface plots	102:114	surface plots	102:114	A study on the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis.
25370022	1	11	theme	melt	207:210	arg1	suspensions					212:222	polymer melt suspensions	199:222	polymer melt suspensions	199:222	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	3	12	theme	principal	561:569	arg1	method					553:558	the statistical method	537:558	the statistical method	537:558	Therefore, the statistical method, principal component analysis (PCA), was explored in this study.
25370022	3	12	theme	principal	561:569	arg1	PCA					591:593	PCA	591:593	PCA	591:593	Therefore, the statistical method, principal component analysis (PCA), was explored in this study.
25370022	3	12	theme	principal	561:569	arg1	analysis					581:588	principal component analysis	561:588	principal component analysis (PCA)	561:594	Therefore, the statistical method, principal component analysis (PCA), was explored in this study.
25370022	2	13	from	formulation	478:488	arg1	grades					451:456	the various grades	439:456	the various grades of adjuvants in the formulation	439:488	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	6	14	theme	suspensions	1143:1153	arg1	viscosity					1125:1133	the viscosity	1121:1133	the viscosity of melt suspensions	1121:1153	Particle size and size distribution of HPMC played an important role in the viscosity of melt suspensions.
25370022	4	15	theme	composite	629:637	arg1	formulations					639:650	The composite formulations	625:650	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations	625:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	1	16	from	manufacturing	253:265	arg1	crucial					227:233	crucial	227:233	crucial	227:233	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	1	16	from	manufacturing	253:265	arg1	understanding					153:165	An understanding	150:165	An understanding of the rheological behaviour of polymer melt suspensions	150:222	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	5	17	theme	greater	992:998	arg1	effect					1000:1005	a greater effect	990:1005	a greater effect	990:1005	Surface plots showed that concentration of HPMC had a greater effect on the viscosity compared to temperature.
25370022	9	18	theme	low	1415:1417	arg1	viscosity					1438:1446	low, moderate and high viscosity	1415:1446	low, moderate and high viscosity	1415:1446	The complex viscosity profiles of the various formulations containing HPMC were successfully classified into three clusters of low, moderate and high viscosity.
25370022	4	19	theme	hydroxypropyl	698:710	arg1	HPMC					729:732	HPMC	729:732	HPMC	729:732	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	19	theme	hydroxypropyl	698:710	arg1	methylcellulose					712:726	hydroxypropyl methylcellulose	698:726	hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations	698:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	10	20	theme	HPMC	1556:1559	arg1	concentration					1539:1551	concentration	1539:1551	concentration	1539:1551	Formulations within each group showed similar viscosities despite differences in grade or concentration of HPMC.
25370022	10	20	theme	HPMC	1556:1559	arg1	grade					1530:1534	grade	1530:1534	grade	1530:1534	Formulations within each group showed similar viscosities despite differences in grade or concentration of HPMC.
25370022	0	21	theme	component	130:138	arg1	analysis					140:147	principal component analysis	120:147	principal component analysis	120:147	A study on the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis.
25370022	9	22	theme	moderate	1420:1427	arg1	viscosity					1438:1446	low, moderate and high viscosity	1415:1446	low, moderate and high viscosity	1415:1446	The complex viscosity profiles of the various formulations containing HPMC were successfully classified into three clusters of low, moderate and high viscosity.
25370022	9	23	theme	formulations	1334:1345	arg1	profiles					1310:1317	The complex viscosity profiles	1288:1317	The complex viscosity profiles of the various formulations containing HPMC	1288:1361	The complex viscosity profiles of the various formulations containing HPMC were successfully classified into three clusters of low, moderate and high viscosity.
25370022	9	24	theme	high	1433:1436	arg1	viscosity					1438:1446	low, moderate and high viscosity	1415:1446	low, moderate and high viscosity	1415:1446	The complex viscosity profiles of the various formulations containing HPMC were successfully classified into three clusters of low, moderate and high viscosity.
25370022	12	25	contain	containing	1719:1728	arg2	HPMC					1730:1733	HPMC	1730:1733	HPMC	1730:1733	PCA was able to differentiate the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner and provided an excellent visualisation of the data.
25370022	12	25	contain	containing	1719:1728	arg1	formulations					1706:1717	different formulations	1696:1717	different formulations containing HPMC in an efficient and time-saving manner	1696:1772	PCA was able to differentiate the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner and provided an excellent visualisation of the data.
25370022	4	26	theme	various	841:847	arg1	concentrations					849:862	various concentrations	841:862	various concentrations	841:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	27	theme	grades	752:757	arg1	HPMC					729:732	HPMC	729:732	HPMC	729:732	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	27	theme	grades	752:757	arg1	methylcellulose					712:726	hydroxypropyl methylcellulose	698:726	hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations	698:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	27	theme	grades	752:757	arg1	PEG					684:686	PEG	684:686	PEG	684:686	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	27	theme	grades	752:757	arg1	glycol					676:681	polyethylene glycol (PEG) 3350	663:692	polyethylene glycol (PEG) 3350	663:692	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	0	28	theme	PEG	75:77	arg1	suspensions					84:94	PEG melt suspensions	75:94	PEG melt suspensions using surface plots and principal component analysis	75:147	A study on the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis.
25370022	8	29	theme	different	1265:1273	arg1	viscosities					1275:1285	different viscosities	1265:1285	different viscosities	1265:1285	PCA was used to evaluate formulations of different viscosities.
25370022	0	30	theme	suspensions	84:94	arg1	viscosity					62:70	the viscosity	58:70	the viscosity of PEG melt suspensions using surface plots and principal component analysis	58:147	A study on the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis.
25370022	0	31	from	impact	15:20	arg1	viscosity					62:70	the viscosity	58:70	the viscosity of PEG melt suspensions using surface plots and principal component analysis	58:147	A study on the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis.
25370022	4	32	theme	different	903:911	arg1	temperatures					913:924	different temperatures	903:924	different temperatures	903:924	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	5	33	theme	Surface	938:944	arg1	plots					946:950	Surface plots	938:950	Surface plots	938:950	Surface plots showed that concentration of HPMC had a greater effect on the viscosity compared to temperature.
25370022	7	34	theme	larger	1206:1211	arg1	particles					1213:1221	larger particles	1206:1221	larger particles	1206:1221	Smaller particles led to a greater viscosity than larger particles.
25370022	2	35	theme	adjuvants	461:469	arg1	grades					451:456	the various grades	439:456	the various grades of adjuvants in the formulation	439:488	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	0	36	theme	hydroxypropyl	25:37	arg1	methylcellulose					39:53	hydroxypropyl methylcellulose	25:53	hydroxypropyl methylcellulose	25:53	A study on the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis.
25370022	12	37	theme	viscosity	1674:1682	arg1	profiles					1684:1691	the complex viscosity profiles	1662:1691	the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner	1662:1772	PCA was able to differentiate the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner and provided an excellent visualisation of the data.
25370022	4	38	from	methylcellulose	712:726	arg1	concentrations					849:862	various concentrations	841:862	various concentrations	841:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	1	39	theme	suspensions	212:222	arg1	behaviour					186:194	the rheological behaviour	170:194	the rheological behaviour of polymer melt suspensions	170:222	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	10	40	theme	similar	1487:1493	arg1	viscosities					1495:1505	similar viscosities	1487:1505	similar viscosities	1487:1505	Formulations within each group showed similar viscosities despite differences in grade or concentration of HPMC.
25370022	1	41	theme	pharmaceutical	238:251	arg1	manufacturing					253:265	pharmaceutical manufacturing	238:265	pharmaceutical manufacturing	238:265	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	12	42	theme	formulations	1706:1717	arg1	profiles					1684:1691	the complex viscosity profiles	1662:1691	the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner	1662:1772	PCA was able to differentiate the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner and provided an excellent visualisation of the data.
25370022	7	43	theme	Smaller	1156:1162	arg1	particles					1164:1172	Smaller particles	1156:1172	Smaller particles	1156:1172	Smaller particles led to a greater viscosity than larger particles.
25370022	11	44	theme	low	1582:1584	arg1	cluster					1596:1602	the low viscosity cluster	1578:1602	the low viscosity cluster	1578:1602	Formulations in the low viscosity cluster were found to be sprayable.
25370022	3	45	theme	statistical	541:551	arg1	method					553:558	the statistical method	537:558	the statistical method	537:558	Therefore, the statistical method, principal component analysis (PCA), was explored in this study.
25370022	3	45	theme	statistical	541:551	arg1	analysis					581:588	principal component analysis	561:588	principal component analysis (PCA)	561:594	Therefore, the statistical method, principal component analysis (PCA), was explored in this study.
25370022	7	46	theme	greater	1183:1189	arg1	viscosity					1191:1199	a greater viscosity	1181:1199	a greater viscosity than larger particles	1181:1221	Smaller particles led to a greater viscosity than larger particles.
25370022	2	47	from	comparison	353:362	arg1	each					386:389	each	386:389	each	386:389	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	2	47	from	comparison	353:362	arg1	every					395:399	every	395:399	every	395:399	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	9	48	contain	containing	1347:1356	arg2	HPMC					1358:1361	HPMC	1358:1361	HPMC	1358:1361	The complex viscosity profiles of the various formulations containing HPMC were successfully classified into three clusters of low, moderate and high viscosity.
25370022	9	48	contain	containing	1347:1356	arg1	formulations					1334:1345	the various formulations	1322:1345	the various formulations containing HPMC	1322:1361	The complex viscosity profiles of the various formulations containing HPMC were successfully classified into three clusters of low, moderate and high viscosity.
25370022	3	49	theme	component	571:579	arg1	method					553:558	the statistical method	537:558	the statistical method	537:558	Therefore, the statistical method, principal component analysis (PCA), was explored in this study.
25370022	3	49	theme	component	571:579	arg1	PCA					591:593	PCA	591:593	PCA	591:593	Therefore, the statistical method, principal component analysis (PCA), was explored in this study.
25370022	3	49	theme	component	571:579	arg1	analysis					581:588	principal component analysis	561:588	principal component analysis (PCA)	561:594	Therefore, the statistical method, principal component analysis (PCA), was explored in this study.
25370022	11	50	from	Formulations	1562:1573	arg1	cluster					1596:1602	the low viscosity cluster	1578:1602	the low viscosity cluster	1578:1602	Formulations in the low viscosity cluster were found to be sprayable.
25370022	2	51	from	grades	451:456	arg1	formulation					478:488	the formulation	474:488	the formulation	474:488	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	6	52	theme	size	1067:1070	arg1	distribution					1072:1083	size distribution	1067:1083	size distribution	1067:1083	Particle size and size distribution of HPMC played an important role in the viscosity of melt suspensions.
25370022	4	53	theme	polyethylene	663:674	arg1	PEG					684:686	PEG	684:686	PEG	684:686	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	53	theme	polyethylene	663:674	arg1	glycol					676:681	polyethylene glycol (PEG) 3350	663:692	polyethylene glycol (PEG) 3350	663:692	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	1	54	theme	behaviour	186:194	arg1	crucial					227:233	crucial	227:233	crucial	227:233	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	1	54	theme	behaviour	186:194	arg1	understanding					153:165	An understanding	150:165	An understanding of the rheological behaviour of polymer melt suspensions	150:222	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	0	55	theme	principal	120:128	arg1	analysis					140:147	principal component analysis	120:147	principal component analysis	120:147	A study on the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis.
25370022	1	56	theme	polymer	199:205	arg1	suspensions					212:222	polymer melt suspensions	199:222	polymer melt suspensions	199:222	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	6	57	theme	melt	1138:1141	arg1	suspensions					1143:1153	melt suspensions	1138:1153	melt suspensions	1138:1153	Particle size and size distribution of HPMC played an important role in the viscosity of melt suspensions.
25370022	1	58	theme	extruding	322:330	arg1	melt					317:320	melt extruding	317:330	melt extruding	317:330	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	1	59	from	crucial	227:233	arg1	manufacturing					253:265	pharmaceutical manufacturing	238:265	pharmaceutical manufacturing	238:265	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	4	60	theme	F50	808:810	arg1	grades					752:757	ten different grades	738:757	ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations	738:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	60	theme	F50	808:810	arg1	LV					812:813	F50 LV	808:813	F50 LV	808:813	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	8	61	used	used	1232:1235	arg2	PCA					1224:1226	PCA	1224:1226	PCA	1224:1226	PCA was used to evaluate formulations of different viscosities.
25370022	5	62	theme	HPMC	981:984	arg1	concentration					964:976	concentration	964:976	concentration of HPMC	964:984	Surface plots showed that concentration of HPMC had a greater effect on the viscosity compared to temperature.
25370022	9	63	theme	viscosity	1438:1446	arg1	clusters					1403:1410	three clusters	1397:1410	three clusters of low, moderate and high viscosity	1397:1446	The complex viscosity profiles of the various formulations containing HPMC were successfully classified into three clusters of low, moderate and high viscosity.
25370022	6	64	theme	important	1103:1111	arg1	role					1113:1116	an important role	1100:1116	an important role	1100:1116	Particle size and size distribution of HPMC played an important role in the viscosity of melt suspensions.
25370022	9	65	theme	viscosity	1300:1308	arg1	profiles					1310:1317	The complex viscosity profiles	1288:1317	The complex viscosity profiles of the various formulations containing HPMC	1288:1361	The complex viscosity profiles of the various formulations containing HPMC were successfully classified into three clusters of low, moderate and high viscosity.
25370022	4	66	dep	grades	752:757	arg1	K4M					769:771	K4M	769:771	K4M	769:771	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	66	dep	grades	752:757	arg1	VLV					833:835	Methocel VLV	824:835	Methocel VLV	824:835	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	66	dep	grades	752:757	arg1	LV					791:792	E15 LV	787:792	E15 LV	787:792	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	66	dep	grades	752:757	arg1	grades					752:757	ten different grades	738:757	ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations	738:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	66	dep	grades	752:757	arg1	LV					812:813	F50 LV	808:813	F50 LV	808:813	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	66	dep	grades	752:757	arg1	F4M					816:818	F4M	816:818	F4M	816:818	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	66	dep	grades	752:757	arg1	K100M					780:784	K100M	780:784	K100M	780:784	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	66	dep	grades	752:757	arg1	LV					799:800	LV	799:800	LV	799:800	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	66	dep	grades	752:757	arg1	LV					765:766	LV	765:766	LV	765:766	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	66	dep	grades	752:757	arg1	E4M					803:805	E4M	803:805	E4M	803:805	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	0	67	from	study	2:6	arg1	impact					15:20	the impact	11:20	the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis	11:147	A study on the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis.
25370022	0	68	theme	melt	79:82	arg1	suspensions					84:94	PEG melt suspensions	75:94	PEG melt suspensions using surface plots and principal component analysis	75:147	A study on the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis.
25370022	2	69	from	adjuvants	461:469	arg1	formulation					478:488	the formulation	474:488	the formulation	474:488	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	4	70	theme	different	742:750	arg1	K4M					769:771	K4M	769:771	K4M	769:771	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	70	theme	different	742:750	arg1	VLV					833:835	Methocel VLV	824:835	Methocel VLV	824:835	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	70	theme	different	742:750	arg1	LV					791:792	E15 LV	787:792	E15 LV	787:792	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	70	theme	different	742:750	arg1	grades					752:757	ten different grades	738:757	ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations	738:862	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	70	theme	different	742:750	arg1	LV					812:813	F50 LV	808:813	F50 LV	808:813	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	70	theme	different	742:750	arg1	F4M					816:818	F4M	816:818	F4M	816:818	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	70	theme	different	742:750	arg1	K100M					780:784	K100M	780:784	K100M	780:784	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	70	theme	different	742:750	arg1	LV					799:800	LV	799:800	LV	799:800	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	70	theme	different	742:750	arg1	LV					765:766	LV	765:766	LV	765:766	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	4	70	theme	different	742:750	arg1	E4M					803:805	E4M	803:805	E4M	803:805	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	2	71	theme	various	443:449	arg1	grades					451:456	the various grades	439:456	the various grades of adjuvants in the formulation	439:488	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	8	72	theme	viscosities	1275:1285	arg1	formulations					1249:1260	formulations	1249:1260	formulations of different viscosities	1249:1285	PCA was used to evaluate formulations of different viscosities.
25370022	2	73	theme	viscosities	371:381	arg1	comparison					353:362	a detailed comparison	342:362	a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation	342:488	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	2	73	theme	viscosities	371:381	arg1	tedious					498:504	tedious	498:504	tedious	498:504	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	12	74	theme	data	1821:1824	arg1	visualisation					1800:1812	an excellent visualisation	1787:1812	an excellent visualisation of the data	1787:1824	PCA was able to differentiate the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner and provided an excellent visualisation of the data.
25370022	5	75	contain	had	986:988	arg2	effect					1000:1005	a greater effect	990:1005	a greater effect	990:1005	Surface plots showed that concentration of HPMC had a greater effect on the viscosity compared to temperature.
25370022	5	75	contain	had	986:988	arg1	concentration					964:976	concentration	964:976	concentration of HPMC	964:984	Surface plots showed that concentration of HPMC had a greater effect on the viscosity compared to temperature.
25370022	1	76	theme	spray	297:301	arg1	congealing					303:312	spray congealing	297:312	spray congealing	297:312	An understanding of the rheological behaviour of polymer melt suspensions is crucial in pharmaceutical manufacturing, especially when processed by spray congealing or melt extruding.
25370022	0	77	theme	methylcellulose	39:53	arg1	impact					15:20	the impact	11:20	the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis	11:147	A study on the impact of hydroxypropyl methylcellulose on the viscosity of PEG melt suspensions using surface plots and principal component analysis.
25370022	4	78	from	temperatures	913:924	arg1	viscosities					888:898	their viscosities	882:898	their viscosities at different temperatures	882:924	The composite formulations comprising polyethylene glycol (PEG) 3350 and hydroxypropyl methylcellulose (HPMC) of ten different grades (K100 LV, K4M, K15M, K100M, E15 LV, E50 LV, E4M, F50 LV, F4M and Methocel VLV) at various concentrations were prepared and their viscosities at different temperatures determined.
25370022	12	79	theme	excellent	1790:1798	arg1	visualisation					1800:1812	an excellent visualisation	1787:1812	an excellent visualisation of the data	1787:1824	PCA was able to differentiate the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner and provided an excellent visualisation of the data.
25370022	12	80	theme	time-saving	1755:1765	arg1	manner					1767:1772	an efficient and time-saving manner	1738:1772	an efficient and time-saving manner	1738:1772	PCA was able to differentiate the complex viscosity profiles of different formulations containing HPMC in an efficient and time-saving manner and provided an excellent visualisation of the data.
25370022	6	81	theme	Particle	1049:1056	arg1	size					1058:1061	Particle size	1049:1061	Particle size	1049:1061	Particle size and size distribution of HPMC played an important role in the viscosity of melt suspensions.
25370022	9	82	theme	complex	1292:1298	arg1	profiles					1310:1317	The complex viscosity profiles	1288:1317	The complex viscosity profiles of the various formulations containing HPMC	1288:1361	The complex viscosity profiles of the various formulations containing HPMC were successfully classified into three clusters of low, moderate and high viscosity.
25370022	2	83	from	each	386:389	arg1	comparison					353:362	a detailed comparison	342:362	a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation	342:488	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	2	83	from	each	386:389	arg1	viscosities					371:381	the viscosities	367:381	the viscosities at each and every temperature and concentration	367:429	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	2	83	from	each	386:389	arg1	tedious					498:504	tedious	498:504	tedious	498:504	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	10	84	from	differences	1515:1525	arg1	concentration					1539:1551	concentration	1539:1551	concentration	1539:1551	Formulations within each group showed similar viscosities despite differences in grade or concentration of HPMC.
25370022	10	84	from	differences	1515:1525	arg1	grade					1530:1534	grade	1530:1534	grade	1530:1534	Formulations within each group showed similar viscosities despite differences in grade or concentration of HPMC.
25370022	2	85	from	every	395:399	arg1	comparison					353:362	a detailed comparison	342:362	a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation	342:488	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	2	85	from	every	395:399	arg1	viscosities					371:381	the viscosities	367:381	the viscosities at each and every temperature and concentration	367:429	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	2	85	from	every	395:399	arg1	tedious					498:504	tedious	498:504	tedious	498:504	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	11	86	theme	viscosity	1586:1594	arg1	cluster					1596:1602	the low viscosity cluster	1578:1602	the low viscosity cluster	1578:1602	Formulations in the low viscosity cluster were found to be sprayable.
25370022	2	87	dep	each	386:389	arg1	temperature					401:411	temperature	401:411	temperature	401:411	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
25370022	2	87	dep	each	386:389	arg1	concentration					417:429	concentration	417:429	concentration	417:429	However, a detailed comparison of the viscosities at each and every temperature and concentration between the various grades of adjuvants in the formulation will be tedious and time-consuming.
27178925	7	0	theme	mechanical	1202:1211	arg1	properties					1213:1222	mechanical properties	1202:1222	mechanical properties	1202:1222	Thus, TO-CNF appeared to be a "green" nanofiller that can simultaneously improve swelling and mechanical properties of PNIPAm hydrogel.
27178925	6	1	theme	compression	1052:1062	arg1	properties					1064:1073	much improved compression properties	1038:1073	much improved compression properties	1038:1073	Also, the composite hydrogels showed superior swelling behavior and much improved compression properties with respect to pure PNIPAm one.
27178925	2	2	theme	compressive	430:440	arg1	strength					442:449	compressive strength	430:449	compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%)	430:532	The microstructure, swelling behaviors, and compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%) were then examined, respectively.
27178925	1	3	theme	anionic	182:188	arg1	groups					202:207	anionic carboxylate groups	182:207	anionic carboxylate groups	182:207	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	6	4	theme	improved	1043:1050	arg1	properties					1064:1073	much improved compression properties	1038:1073	much improved compression properties	1038:1073	Also, the composite hydrogels showed superior swelling behavior and much improved compression properties with respect to pure PNIPAm one.
27178925	7	5	theme	green	1139:1143	arg1	TO-CNF					1114:1119	TO-CNF	1114:1119	TO-CNF	1114:1119	Thus, TO-CNF appeared to be a "green" nanofiller that can simultaneously improve swelling and mechanical properties of PNIPAm hydrogel.
27178925	7	5	theme	green	1139:1143	arg1	nanofiller					1146:1155	a "green" nanofiller	1136:1155	a "green" nanofiller that can simultaneously improve swelling and mechanical properties of PNIPAm hydrogel	1136:1241	Thus, TO-CNF appeared to be a "green" nanofiller that can simultaneously improve swelling and mechanical properties of PNIPAm hydrogel.
27178925	3	6	theme	similar	601:607	arg1	transparency					615:626	the similar light transparency	597:626	the similar light transparency to pure PNIPAm one	597:645	Modified hydrogels exhibited the similar light transparency to pure PNIPAm one due to the formation of semi-IPN structure between PNIPAm and TO-CNF.
27178925	7	7	theme	"	1144:1144	arg1	TO-CNF					1114:1119	TO-CNF	1114:1119	TO-CNF	1114:1119	Thus, TO-CNF appeared to be a "green" nanofiller that can simultaneously improve swelling and mechanical properties of PNIPAm hydrogel.
27178925	7	7	theme	"	1144:1144	arg1	nanofiller					1146:1155	a "green" nanofiller	1136:1155	a "green" nanofiller that can simultaneously improve swelling and mechanical properties of PNIPAm hydrogel	1136:1241	Thus, TO-CNF appeared to be a "green" nanofiller that can simultaneously improve swelling and mechanical properties of PNIPAm hydrogel.
27178925	5	8	theme	PNIPAm	895:900	arg1	hydrogels					902:910	PNIPAm hydrogels	895:910	PNIPAm hydrogels	895:910	SEM observation suggested that the pore size of PNIPAm hydrogels was markedly increased after the incorporation of TO-CNF.
27178925	7	9	theme	PNIPAm	1227:1232	arg1	hydrogel					1234:1241	PNIPAm hydrogel	1227:1241	PNIPAm hydrogel	1227:1241	Thus, TO-CNF appeared to be a "green" nanofiller that can simultaneously improve swelling and mechanical properties of PNIPAm hydrogel.
27178925	1	10	with	nanofibers	157:166	arg1	groups					202:207	anionic carboxylate groups	182:207	anionic carboxylate groups	182:207	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	5	11	theme	pore	882:885	arg1	size					887:890	the pore size	878:890	the pore size of PNIPAm hydrogels	878:910	SEM observation suggested that the pore size of PNIPAm hydrogels was markedly increased after the incorporation of TO-CNF.
27178925	4	12	theme	peaks	817:821	arg1	position					790:797	the position	786:797	the position of characteristic peaks associated with PNIPAm	786:844	FT-IR spectra demonstrated that the presence of TO-CNF did not alter the position of characteristic peaks associated with PNIPAm.
27178925	1	13	theme	carboxylate	190:200	arg1	groups					202:207	anionic carboxylate groups	182:207	anionic carboxylate groups	182:207	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	7	14	theme	hydrogel	1234:1241	arg1	swelling					1189:1196	swelling	1189:1196	swelling	1189:1196	Thus, TO-CNF appeared to be a "green" nanofiller that can simultaneously improve swelling and mechanical properties of PNIPAm hydrogel.
27178925	7	14	theme	hydrogel	1234:1241	arg1	properties					1213:1222	mechanical properties	1202:1222	mechanical properties	1202:1222	Thus, TO-CNF appeared to be a "green" nanofiller that can simultaneously improve swelling and mechanical properties of PNIPAm hydrogel.
27178925	5	15	theme	SEM	847:849	arg1	observation					851:861	SEM observation	847:861	SEM observation	847:861	SEM observation suggested that the pore size of PNIPAm hydrogels was markedly increased after the incorporation of TO-CNF.
27178925	1	16	from	nanofibers	157:166	arg1	surfaces					216:223	the surfaces	212:223	the surfaces	212:223	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	3	17	theme	Modified	568:575	arg1	hydrogels					577:585	Modified hydrogels	568:585	Modified hydrogels	568:585	Modified hydrogels exhibited the similar light transparency to pure PNIPAm one due to the formation of semi-IPN structure between PNIPAm and TO-CNF.
27178925	5	18	theme	hydrogels	902:910	arg1	size					887:890	the pore size	878:890	the pore size of PNIPAm hydrogels	878:910	SEM observation suggested that the pore size of PNIPAm hydrogels was markedly increased after the incorporation of TO-CNF.
27178925	2	19	theme	varying	496:502	arg1	contents					504:511	varying contents	496:511	varying contents of TO-CNFs (0-10wt%)	496:532	The microstructure, swelling behaviors, and compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%) were then examined, respectively.
27178925	3	20	theme	light	609:613	arg1	transparency					615:626	the similar light transparency	597:626	the similar light transparency to pure PNIPAm one	597:645	Modified hydrogels exhibited the similar light transparency to pure PNIPAm one due to the formation of semi-IPN structure between PNIPAm and TO-CNF.
27178925	0	21	theme	Thermo-responsive	0:16	arg1	properties					34:43	Thermo-responsive and compression properties	0:43	Thermo-responsive and compression properties of TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels.	0:108	Thermo-responsive and compression properties of TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels.
27178925	2	22	theme	hydrogels	481:489	arg1	microstructure					390:403	The microstructure	386:403	The microstructure	386:403	The microstructure, swelling behaviors, and compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%) were then examined, respectively.
27178925	2	22	theme	hydrogels	481:489	arg1	behaviors					415:423	swelling behaviors	406:423	swelling behaviors	406:423	The microstructure, swelling behaviors, and compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%) were then examined, respectively.
27178925	2	22	theme	hydrogels	481:489	arg1	strength					442:449	compressive strength	430:449	compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%)	430:532	The microstructure, swelling behaviors, and compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%) were then examined, respectively.
27178925	6	23	theme	swelling	1016:1023	arg1	behavior					1025:1032	superior swelling behavior	1007:1032	superior swelling behavior	1007:1032	Also, the composite hydrogels showed superior swelling behavior and much improved compression properties with respect to pure PNIPAm one.
27178925	2	24	theme	PNIPAm	464:469	arg1	hydrogels					481:489	resultant PNIPAm composite hydrogels	454:489	resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%)	454:532	The microstructure, swelling behaviors, and compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%) were then examined, respectively.
27178925	0	25	theme	compression	22:32	arg1	properties					34:43	Thermo-responsive and compression properties	0:43	Thermo-responsive and compression properties of TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels.	0:108	Thermo-responsive and compression properties of TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels.
27178925	6	26	theme	superior	1007:1014	arg1	behavior					1025:1032	superior swelling behavior	1007:1032	superior swelling behavior	1007:1032	Also, the composite hydrogels showed superior swelling behavior and much improved compression properties with respect to pure PNIPAm one.
27178925	3	27	theme	structure	680:688	arg1	formation					658:666	the formation	654:666	the formation of semi-IPN structure between PNIPAm and TO-CNF	654:714	Modified hydrogels exhibited the similar light transparency to pure PNIPAm one due to the formation of semi-IPN structure between PNIPAm and TO-CNF.
27178925	1	28	theme	TEMPO-oxidized	125:138	arg1	nanofibers					157:166	TEMPO-oxidized bamboo cellulose nanofibers	125:166	TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces	125:223	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	1	28	theme	TEMPO-oxidized	125:138	arg1	TO-CNF					169:174	TO-CNF	169:174	TO-CNF	169:174	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	2	29	theme	resultant	454:462	arg1	hydrogels					481:489	resultant PNIPAm composite hydrogels	454:489	resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%)	454:532	The microstructure, swelling behaviors, and compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%) were then examined, respectively.
27178925	0	30	theme	TEMPO-oxidized	48:61	arg1	hydrogels					99:107	TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels	48:107	TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels	48:107	Thermo-responsive and compression properties of TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels.
27178925	5	31	theme	TO-CNF	962:967	arg1	incorporation					945:957	the incorporation	941:957	the incorporation of TO-CNF	941:967	SEM observation suggested that the pore size of PNIPAm hydrogels was markedly increased after the incorporation of TO-CNF.
27178925	2	32	theme	TO-CNFs	516:522	arg1	contents					504:511	varying contents	496:511	varying contents of TO-CNFs (0-10wt%)	496:532	The microstructure, swelling behaviors, and compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%) were then examined, respectively.
27178925	1	33	theme	thermo-responsive	315:331	arg1	properties					348:357	its thermo-responsive and mechanical properties	311:357	its thermo-responsive and mechanical properties	311:357	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	2	34	with	hydrogels	481:489	arg1	contents					504:511	varying contents	496:511	varying contents of TO-CNFs (0-10wt%)	496:532	The microstructure, swelling behaviors, and compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%) were then examined, respectively.
27178925	6	35	theme	pure	1091:1094	arg1	PNIPAm					1096:1101	pure PNIPAm one	1091:1105	pure PNIPAm one	1091:1105	Also, the composite hydrogels showed superior swelling behavior and much improved compression properties with respect to pure PNIPAm one.
27178925	0	36	theme	nanofiber-modified	73:90	arg1	hydrogels					99:107	TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels	48:107	TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels	48:107	Thermo-responsive and compression properties of TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels.
27178925	6	37	theme	composite	980:988	arg1	hydrogels					990:998	the composite hydrogels	976:998	the composite hydrogels	976:998	Also, the composite hydrogels showed superior swelling behavior and much improved compression properties with respect to pure PNIPAm one.
27178925	3	38	theme	semi-IPN	671:678	arg1	structure					680:688	semi-IPN structure	671:688	semi-IPN structure	671:688	Modified hydrogels exhibited the similar light transparency to pure PNIPAm one due to the formation of semi-IPN structure between PNIPAm and TO-CNF.
27178925	1	39	theme	bamboo	140:145	arg1	nanofibers					157:166	TEMPO-oxidized bamboo cellulose nanofibers	125:166	TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces	125:223	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	1	39	theme	bamboo	140:145	arg1	TO-CNF					169:174	TO-CNF	169:174	TO-CNF	169:174	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	0	40	theme	cellulose	63:71	arg1	hydrogels					99:107	TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels	48:107	TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels	48:107	Thermo-responsive and compression properties of TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels.
27178925	1	41	theme	mechanical	337:346	arg1	properties					348:357	its thermo-responsive and mechanical properties	311:357	its thermo-responsive and mechanical properties	311:357	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	3	42	theme	pure	631:634	arg1	PNIPAm					636:641	pure PNIPAm one	631:645	pure PNIPAm one	631:645	Modified hydrogels exhibited the similar light transparency to pure PNIPAm one due to the formation of semi-IPN structure between PNIPAm and TO-CNF.
27178925	1	43	theme	cellulose	147:155	arg1	nanofibers					157:166	TEMPO-oxidized bamboo cellulose nanofibers	125:166	TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces	125:223	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	1	43	theme	cellulose	147:155	arg1	TO-CNF					169:174	TO-CNF	169:174	TO-CNF	169:174	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	4	44	theme	TO-CNF	765:770	arg1	presence					753:760	the presence	749:760	the presence of TO-CNF	749:770	FT-IR spectra demonstrated that the presence of TO-CNF did not alter the position of characteristic peaks associated with PNIPAm.
27178925	4	45	theme	characteristic	802:815	arg1	peaks					817:821	characteristic peaks	802:821	characteristic peaks associated with PNIPAm	802:844	FT-IR spectra demonstrated that the presence of TO-CNF did not alter the position of characteristic peaks associated with PNIPAm.
27178925	1	46	theme	poly	256:259	arg1	matrix					293:298	poly(N-isopropylacrylamide) (PNIPAm) matrix	256:298	poly(N-isopropylacrylamide) (PNIPAm) matrix	256:298	In this study, TEMPO-oxidized bamboo cellulose nanofibers (TO-CNF) with anionic carboxylate groups on the surfaces were in-situ incorporated into poly(N-isopropylacrylamide) (PNIPAm) matrix to improve its thermo-responsive and mechanical properties during the polymerization.
27178925	0	47	theme	hydrogels	99:107	arg1	properties					34:43	Thermo-responsive and compression properties	0:43	Thermo-responsive and compression properties of TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels.	0:108	Thermo-responsive and compression properties of TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels.
27178925	2	48	theme	swelling	406:413	arg1	behaviors					415:423	swelling behaviors	406:423	swelling behaviors	406:423	The microstructure, swelling behaviors, and compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%) were then examined, respectively.
27178925	0	49	theme	PNIPAm	92:97	arg1	hydrogels					99:107	TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels	48:107	TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels	48:107	Thermo-responsive and compression properties of TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels.
27178925	2	50	theme	composite	471:479	arg1	hydrogels					481:489	resultant PNIPAm composite hydrogels	454:489	resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%)	454:532	The microstructure, swelling behaviors, and compressive strength of resultant PNIPAm composite hydrogels with varying contents of TO-CNFs (0-10wt%) were then examined, respectively.
27178925	4	51	theme	FT-IR	717:721	arg1	spectra					723:729	FT-IR spectra	717:729	FT-IR spectra	717:729	FT-IR spectra demonstrated that the presence of TO-CNF did not alter the position of characteristic peaks associated with PNIPAm.
26256338	1	0	theme	sandwich	187:194	arg1	nanofibers					196:205	clotrimazole (CZ)-composite sandwich nanofibers	159:205	clotrimazole (CZ)-composite sandwich nanofibers using electrospinning	159:227	This study aims to fabricate clotrimazole (CZ)-composite sandwich nanofibers using electrospinning.
26256338	5	1	theme	good	808:811	arg1	properties					826:835	good mucoadhesive properties	808:835	good mucoadhesive properties	808:835	The fibers were in the nanoscale with good mucoadhesive properties.
26256338	8	2	theme	release	1056:1062	arg1	rates					1064:1068	slower release rates	1049:1068	slower release rates	1049:1068	Moreover, the sandwich nanofibers coated for longer times resulted in slower release rates compared with the shorter coating times.
26256338	6	3	theme	molecular	858:866	arg1	dispersion					868:877	a molecular dispersion	856:877	a molecular dispersion of amorphous CZ in the nanofibers	856:911	The XRPD revealed a molecular dispersion of amorphous CZ in the nanofibers.
26256338	2	4	from	nanofibers	476:485	arg1	release					447:453	a sustained release	435:453	a sustained release of the drug from the nanofibers	435:485	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	3	5	theme	X-ray	616:620	arg1	XRD					638:640	XRD	638:640	XRD	638:640	The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD).
26256338	3	5	theme	X-ray	616:620	arg1	diffractometry					622:635	X-ray diffractometry	616:635	X-ray diffractometry (XRD)	616:641	The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD).
26256338	2	6	theme	CS-SH	349:353	arg1	PVA					375:377	PVA	375:377	PVA	375:377	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	2	6	theme	CS-SH	349:353	arg1	alcohol					366:372	chitosan-cysteine (CS-SH)/polyvinyl alcohol	330:372	chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA)	330:378	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	7	7	theme	initial	918:924	arg1	release					931:937	The initial fast release	914:937	The initial fast release of CZ from the nanofibers	914:963	The initial fast release of CZ from the nanofibers was achieved.
26256338	7	8	from	nanofibers	954:963	arg1	release					931:937	The initial fast release	914:937	The initial fast release of CZ from the nanofibers	914:963	The initial fast release of CZ from the nanofibers was achieved.
26256338	8	9	theme	longer	1024:1029	arg1	times					1031:1035	longer times	1024:1035	longer times	1024:1035	Moreover, the sandwich nanofibers coated for longer times resulted in slower release rates compared with the shorter coating times.
26256338	2	10	theme	mucoadhesive	396:407	arg1	properties					409:418	the mucoadhesive properties	392:418	the mucoadhesive properties	392:418	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	10	11	theme	promising	1299:1307	arg1	candidates					1309:1318	promising candidates	1299:1318	promising candidates for the treatment of oral candidiasis	1299:1356	Therefore, these nanofibers may be promising candidates for the treatment of oral candidiasis.
26256338	3	12	dep	Fourier	564:570	arg1	transform					572:580	transform	572:580	transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD)	572:641	The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD).
26256338	8	13	theme	shorter	1088:1094	arg1	times					1104:1108	the shorter coating times	1084:1108	the shorter coating times	1084:1108	Moreover, the sandwich nanofibers coated for longer times resulted in slower release rates compared with the shorter coating times.
26256338	2	14	theme	chitosan-cysteine	330:346	arg1	PVA					375:377	PVA	375:377	PVA	375:377	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	2	14	theme	chitosan-cysteine	330:346	arg1	alcohol					366:372	chitosan-cysteine (CS-SH)/polyvinyl alcohol	330:372	chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA)	330:378	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	2	15	theme	sustained	437:445	arg1	release					447:453	a sustained release	435:453	a sustained release of the drug from the nanofibers	435:485	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	5	16	theme	mucoadhesive	813:824	arg1	properties					826:835	good mucoadhesive properties	808:835	good mucoadhesive properties	808:835	The fibers were in the nanoscale with good mucoadhesive properties.
26256338	8	17	dep	resulted	1037:1044	arg1	compared					1070:1077	compared	1070:1077	compared with the shorter coating times	1070:1108	Moreover, the sandwich nanofibers coated for longer times resulted in slower release rates compared with the shorter coating times.
26256338	8	18	theme	coating	1096:1102	arg1	times					1104:1108	the shorter coating times	1084:1108	the shorter coating times	1084:1108	Moreover, the sandwich nanofibers coated for longer times resulted in slower release rates compared with the shorter coating times.
26256338	8	19	theme	slower	1049:1054	arg1	rates					1064:1068	slower release rates	1049:1068	slower release rates	1049:1068	Moreover, the sandwich nanofibers coated for longer times resulted in slower release rates compared with the shorter coating times.
26256338	0	20	theme	chitosan	28:35	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of mucoadhesive chitosan	0:35	Fabrication of mucoadhesive chitosan coated polyvinylpyrrolidone/cyclodextrin/clotrimazole sandwich patches for oral candidiasis.
26256338	3	21	theme	FT-IR	592:596	arg1	spectroscopy					599:610	(FT-IR) spectroscopy	591:610	(FT-IR) spectroscopy	591:610	The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD).
26256338	2	22	theme	drug	462:465	arg1	release					447:453	a sustained release	435:453	a sustained release of the drug from the nanofibers	435:485	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	0	23	theme	mucoadhesive	15:26	arg1	chitosan					28:35	mucoadhesive chitosan	15:35	mucoadhesive chitosan	15:35	Fabrication of mucoadhesive chitosan coated polyvinylpyrrolidone/cyclodextrin/clotrimazole sandwich patches for oral candidiasis.
26256338	4	24	theme	mechanical	659:668	arg1	properties					687:696	The nanofibers mechanical and mucoadhesive properties	644:696	The nanofibers mechanical and mucoadhesive properties	644:696	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	4	24	theme	mechanical	659:668	arg1	release					704:710	drug release	699:710	drug release	699:710	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	4	24	theme	mechanical	659:668	arg1	activity					724:731	antifungal activity	713:731	antifungal activity	713:731	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	4	24	theme	mechanical	659:668	arg1	cytotoxicity					737:748	cytotoxicity	737:748	cytotoxicity	737:748	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	0	25	theme	polyvinylpyrrolidone/cyclodextrin/clotrimazole	44:89	arg1	patches					100:106	polyvinylpyrrolidone/cyclodextrin/clotrimazole sandwich patches	44:106	polyvinylpyrrolidone/cyclodextrin/clotrimazole sandwich patches	44:106	Fabrication of mucoadhesive chitosan coated polyvinylpyrrolidone/cyclodextrin/clotrimazole sandwich patches for oral candidiasis.
26256338	3	26	dep	transform	572:580	arg1	infrared					582:589	infrared	582:589	transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD)	572:641	The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD).
26256338	2	27	theme	HPβCD	301:305	arg1	fiber					308:312	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber	230:312	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber	230:312	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	4	28	theme	drug	699:702	arg1	properties					687:696	The nanofibers mechanical and mucoadhesive properties	644:696	The nanofibers mechanical and mucoadhesive properties	644:696	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	4	28	theme	drug	699:702	arg1	release					704:710	drug release	699:710	drug release	699:710	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	6	29	from	dispersion	868:877	arg1	nanofibers					902:911	the nanofibers	898:911	the nanofibers	898:911	The XRPD revealed a molecular dispersion of amorphous CZ in the nanofibers.
26256338	9	30	theme	2-h	1248:1250	arg1	incubation					1252:1261	a 2-h incubation	1246:1261	a 2-h incubation	1246:1261	The CZ-loaded nanofibers killed the Candida significantly faster than the commercial CZ lozenges at 5, 15 and 30 min and were safe for a 2-h incubation.
26256338	9	31	theme	faster	1169:1174	arg1	Candida					1147:1153	the Candida	1143:1153	the Candida significantly faster than the commercial CZ lozenges	1143:1206	The CZ-loaded nanofibers killed the Candida significantly faster than the commercial CZ lozenges at 5, 15 and 30 min and were safe for a 2-h incubation.
26256338	4	32	theme	mucoadhesive	674:685	arg1	properties					687:696	The nanofibers mechanical and mucoadhesive properties	644:696	The nanofibers mechanical and mucoadhesive properties	644:696	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	4	32	theme	mucoadhesive	674:685	arg1	release					704:710	drug release	699:710	drug release	699:710	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	4	32	theme	mucoadhesive	674:685	arg1	activity					724:731	antifungal activity	713:731	antifungal activity	713:731	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	4	32	theme	mucoadhesive	674:685	arg1	cytotoxicity					737:748	cytotoxicity	737:748	cytotoxicity	737:748	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	2	33	theme	/hydroxypropyl-β-cyclodextrin	270:298	arg1	fiber					308:312	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber	230:312	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber	230:312	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	10	34	theme	candidiasis	1346:1356	arg1	treatment					1328:1336	the treatment	1324:1336	the treatment of oral candidiasis	1324:1356	Therefore, these nanofibers may be promising candidates for the treatment of oral candidiasis.
26256338	3	35	theme	electron	537:544	arg1	SEM					558:560	SEM	558:560	SEM	558:560	The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD).
26256338	3	35	theme	electron	537:544	arg1	Fourier					564:570	Fourier	564:570	Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD)	564:641	The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD).
26256338	3	35	theme	electron	537:544	arg1	microscopy					546:555	scanning electron microscopy	528:555	scanning electron microscopy (SEM)	528:561	The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD).
26256338	0	36	theme	sandwich	91:98	arg1	patches					100:106	polyvinylpyrrolidone/cyclodextrin/clotrimazole sandwich patches	44:106	polyvinylpyrrolidone/cyclodextrin/clotrimazole sandwich patches	44:106	Fabrication of mucoadhesive chitosan coated polyvinylpyrrolidone/cyclodextrin/clotrimazole sandwich patches for oral candidiasis.
26256338	6	37	theme	CZ	892:893	arg1	dispersion					868:877	a molecular dispersion	856:877	a molecular dispersion of amorphous CZ in the nanofibers	856:911	The XRPD revealed a molecular dispersion of amorphous CZ in the nanofibers.
26256338	10	38	theme	oral	1341:1344	arg1	candidiasis					1346:1356	oral candidiasis	1341:1356	oral candidiasis	1341:1356	Therefore, these nanofibers may be promising candidates for the treatment of oral candidiasis.
26256338	1	39	theme	clotrimazole	159:170	arg1	nanofibers					196:205	clotrimazole (CZ)-composite sandwich nanofibers	159:205	clotrimazole (CZ)-composite sandwich nanofibers using electrospinning	159:227	This study aims to fabricate clotrimazole (CZ)-composite sandwich nanofibers using electrospinning.
26256338	4	40	theme	antifungal	713:722	arg1	properties					687:696	The nanofibers mechanical and mucoadhesive properties	644:696	The nanofibers mechanical and mucoadhesive properties	644:696	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	4	40	theme	antifungal	713:722	arg1	activity					724:731	antifungal activity	713:731	antifungal activity	713:731	The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed.
26256338	2	41	theme	/polyvinyl	355:364	arg1	PVA					375:377	PVA	375:377	PVA	375:377	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	2	41	theme	/polyvinyl	355:364	arg1	alcohol					366:372	chitosan-cysteine (CS-SH)/polyvinyl alcohol	330:372	chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA)	330:378	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	9	42	theme	commercial	1185:1194	arg1	lozenges					1199:1206	the commercial CZ lozenges	1181:1206	the commercial CZ lozenges	1181:1206	The CZ-loaded nanofibers killed the Candida significantly faster than the commercial CZ lozenges at 5, 15 and 30 min and were safe for a 2-h incubation.
26256338	2	43	theme	polyvinylpyrrolidone	244:263	arg1	fiber					308:312	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber	230:312	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber	230:312	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	9	44	theme	CZ	1196:1197	arg1	lozenges					1199:1206	the commercial CZ lozenges	1181:1206	the commercial CZ lozenges	1181:1206	The CZ-loaded nanofibers killed the Candida significantly faster than the commercial CZ lozenges at 5, 15 and 30 min and were safe for a 2-h incubation.
26256338	3	45	theme	scanning	528:535	arg1	SEM					558:560	SEM	558:560	SEM	558:560	The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD).
26256338	3	45	theme	scanning	528:535	arg1	Fourier					564:570	Fourier	564:570	Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD)	564:641	The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD).
26256338	3	45	theme	scanning	528:535	arg1	microscopy					546:555	scanning electron microscopy	528:555	scanning electron microscopy (SEM)	528:561	The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD).
26256338	9	46	theme	CZ-loaded	1115:1123	arg1	nanofibers					1125:1134	The CZ-loaded nanofibers	1111:1134	The CZ-loaded nanofibers	1111:1134	The CZ-loaded nanofibers killed the Candida significantly faster than the commercial CZ lozenges at 5, 15 and 30 min and were safe for a 2-h incubation.
26256338	9	46	theme	CZ-loaded	1115:1123	arg1	safe					1237:1240	safe	1237:1240	safe	1237:1240	The CZ-loaded nanofibers killed the Candida significantly faster than the commercial CZ lozenges at 5, 15 and 30 min and were safe for a 2-h incubation.
26256338	0	47	theme	oral	112:115	arg1	candidiasis					117:127	oral candidiasis	112:127	oral candidiasis	112:127	Fabrication of mucoadhesive chitosan coated polyvinylpyrrolidone/cyclodextrin/clotrimazole sandwich patches for oral candidiasis.
26256338	2	48	theme	CZ-loaded	234:242	arg1	polyvinylpyrrolidone					244:263	The CZ-loaded polyvinylpyrrolidone	230:263	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber	230:312	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	2	48	theme	CZ-loaded	234:242	arg1	PVP					266:268	PVP	266:268	PVP	266:268	The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers.
26256338	7	49	theme	CZ	942:943	arg1	release					931:937	The initial fast release	914:937	The initial fast release of CZ from the nanofibers	914:963	The initial fast release of CZ from the nanofibers was achieved.
26256338	5	50	with	nanoscale	793:801	arg1	properties					826:835	good mucoadhesive properties	808:835	good mucoadhesive properties	808:835	The fibers were in the nanoscale with good mucoadhesive properties.
26256338	8	51	theme	sandwich	993:1000	arg1	nanofibers					1002:1011	the sandwich nanofibers	989:1011	the sandwich nanofibers coated for longer times	989:1035	Moreover, the sandwich nanofibers coated for longer times resulted in slower release rates compared with the shorter coating times.
26256338	1	52	theme	-composite	176:185	arg1	nanofibers					196:205	clotrimazole (CZ)-composite sandwich nanofibers	159:205	clotrimazole (CZ)-composite sandwich nanofibers using electrospinning	159:227	This study aims to fabricate clotrimazole (CZ)-composite sandwich nanofibers using electrospinning.
26256338	7	53	theme	fast	926:929	arg1	release					931:937	The initial fast release	914:937	The initial fast release of CZ from the nanofibers	914:963	The initial fast release of CZ from the nanofibers was achieved.
28821301	6	0	theme	interactions	1097:1108	arg1	suggestive					1057:1066	suggestive	1057:1066	suggestive	1057:1066	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	3	1	theme	radiation	545:553	arg1	role					537:540	the role	533:540	the role of radiation in altering the microbiome during deep-space travel	533:605	Although the importance of the gut microbiome in the maintenance of human health is well established, little is known about the role of radiation in altering the microbiome during deep-space travel.
28821301	2	2	from	changes	357:363	arg1	microbiome					372:381	the microbiome and host gene expression	368:406	microbiome	372:381	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	2	2	from	changes	357:363	arg1	expression					397:406	the microbiome and host gene expression	368:406	expression	397:406	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	6	3	theme	repair	1180:1185	arg1	processes					1187:1195	the host's cellular damage repair processes	1153:1195	the host's cellular damage repair processes	1153:1195	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	4	4	theme	LET	657:659	arg1	radiation					661:669	high LET radiation	652:669	high LET radiation	652:669	RESULTS Using a mouse model for exposure to high LET radiation, we observed substantial changes in the composition and functional potential of the gut microbiome.
28821301	10	5	theme	potential	2063:2071	arg1	targets					2077:2083	potential new targets	2063:2083	potential new targets for intervention in adverse radiation effects	2063:2129	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	1	6	theme	high	174:177	arg1	radiation					208:216	high linear energy transfer (LET) radiation	174:216	high linear energy transfer (LET) radiation	174:216	BACKGROUND Space travel is associated with continuous low dose rate exposure to high linear energy transfer (LET) radiation.
28821301	10	7	from	effects	2005:2011	arg1	health					2041:2046	human health	2035:2046	human health	2035:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	9	8	theme	biologic	1810:1817	arg1	hazard					1819:1824	an unappreciated biologic hazard	1793:1824	an unappreciated biologic hazard of space travel	1793:1840	CONCLUSIONS The implication of microbiome-mediated pathophysiology after low dose ionizing radiation may be an unappreciated biologic hazard of space travel and deserves experimental validation.
28821301	6	9	theme	cellular	1164:1171	arg1	processes					1187:1195	the host's cellular damage repair processes	1153:1195	the host's cellular damage repair processes	1153:1195	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	10	10	theme	adverse	2105:2111	arg1	effects					2123:2129	adverse radiation effects	2105:2129	adverse radiation effects	2105:2129	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	2	11	theme	radiation	328:336	arg1	changes					357:363	changes	357:363	changes in the microbiome and host gene expression	357:406	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	2	11	theme	radiation	328:336	arg1	effects					338:344	non-cytocidal radiation effects	314:344	non-cytocidal radiation effects	314:344	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	8	12	attach	linked	1617:1622	arg1	changes					1642:1648	dose-dependent changes	1627:1648	dose-dependent changes in the abundance of specific taxa	1627:1682	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	8	12	attach	linked	1617:1622	arg2	activity					1524:1531	the activity	1520:1531	the activity in other radiation-responsive pathways like phosphatidylinositol signaling	1520:1606	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	1	13	theme	energy	186:191	arg1	radiation					208:216	high linear energy transfer (LET) radiation	174:216	high linear energy transfer (LET) radiation	174:216	BACKGROUND Space travel is associated with continuous low dose rate exposure to high linear energy transfer (LET) radiation.
28821301	4	14	theme	functional	727:736	arg1	potential					738:746	functional potential	727:746	functional potential	727:746	RESULTS Using a mouse model for exposure to high LET radiation, we observed substantial changes in the composition and functional potential of the gut microbiome.
28821301	5	15	theme	metabolic	932:940	arg1	modeling					950:957	metabolic network modeling	932:957	metabolic network modeling	932:957	These were accompanied by changes in the abundance of multiple metabolites, which were related to the enzymatic activity of the predicted metagenome by means of metabolic network modeling.
28821301	10	16	theme	conceptual	1902:1911	arg1	basis					1928:1932	a conceptual and analytical basis	1900:1932	a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health	1900:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	5	17	theme	metabolites	834:844	arg1	abundance					812:820	the abundance	808:820	the abundance	808:820	These were accompanied by changes in the abundance of multiple metabolites, which were related to the enzymatic activity of the predicted metagenome by means of metabolic network modeling.
28821301	9	18	theme	travel	1835:1840	arg1	hazard					1819:1824	an unappreciated biologic hazard	1793:1824	an unappreciated biologic hazard of space travel	1793:1840	CONCLUSIONS The implication of microbiome-mediated pathophysiology after low dose ionizing radiation may be an unappreciated biologic hazard of space travel and deserves experimental validation.
28821301	2	19	theme	gene	392:395	arg1	expression					397:406	the microbiome and host gene expression	368:406	expression	397:406	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	2	20	theme	Pathophysiological	219:236	arg1	manifestations					238:251	Pathophysiological manifestations	219:251	Pathophysiological manifestations after low dose radiation exposure	219:285	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	7	21	from	changes	1229:1235	arg1	composition					1265:1275	composition	1265:1275	composition	1265:1275	The observed radiation-induced changes in microbiota diversity and composition were analyzed at the functional level.
28821301	7	21	from	changes	1229:1235	arg1	diversity					1251:1259	microbiota diversity	1240:1259	microbiota diversity	1240:1259	The observed radiation-induced changes in microbiota diversity and composition were analyzed at the functional level.
28821301	8	22	theme	constitutive	1318:1329	arg1	change					1331:1336	A constitutive change	1316:1336	A constitutive change in activity	1316:1348	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	3	23	theme	deep-space	589:598	arg1	travel					600:605	deep-space travel	589:605	deep-space travel	589:605	Although the importance of the gut microbiome in the maintenance of human health is well established, little is known about the role of radiation in altering the microbiome during deep-space travel.
28821301	5	24	theme	metagenome	909:918	arg1	activity					883:890	the enzymatic activity	869:890	the enzymatic activity of the predicted metagenome	869:918	These were accompanied by changes in the abundance of multiple metabolites, which were related to the enzymatic activity of the predicted metagenome by means of metabolic network modeling.
28821301	8	25	theme	other	1536:1540	arg1	pathways					1563:1570	other radiation-responsive pathways	1536:1570	other radiation-responsive pathways like phosphatidylinositol signaling	1536:1606	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	1	26	theme	Space	105:109	arg1	travel					111:116	BACKGROUND Space travel	94:116	BACKGROUND Space travel	94:116	BACKGROUND Space travel is associated with continuous low dose rate exposure to high linear energy transfer (LET) radiation.
28821301	2	27	theme	dose	263:266	arg1	exposure					278:285	low dose radiation exposure	259:285	low dose radiation exposure	259:285	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	1	28	theme	continuous	137:146	arg1	exposure					162:169	continuous low dose rate exposure	137:169	continuous low dose rate exposure to high linear energy transfer (LET) radiation	137:216	BACKGROUND Space travel is associated with continuous low dose rate exposure to high linear energy transfer (LET) radiation.
28821301	8	29	from	activity	1524:1531	arg1	pathways					1563:1570	other radiation-responsive pathways	1536:1570	other radiation-responsive pathways like phosphatidylinositol signaling	1536:1606	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	1	30	theme	dose	152:155	arg1	exposure					162:169	continuous low dose rate exposure	137:169	continuous low dose rate exposure to high linear energy transfer (LET) radiation	137:216	BACKGROUND Space travel is associated with continuous low dose rate exposure to high linear energy transfer (LET) radiation.
28821301	8	31	theme	phosphatidylinositol	1577:1596	arg1	signaling					1598:1606	phosphatidylinositol signaling	1577:1606	phosphatidylinositol signaling	1577:1606	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	7	32	theme	microbiota	1240:1249	arg1	diversity					1251:1259	microbiota diversity	1240:1259	microbiota diversity	1240:1259	The observed radiation-induced changes in microbiota diversity and composition were analyzed at the functional level.
28821301	4	33	theme	microbiome	759:768	arg1	composition					711:721	composition	711:721	composition	711:721	RESULTS Using a mouse model for exposure to high LET radiation, we observed substantial changes in the composition and functional potential of the gut microbiome.
28821301	4	33	theme	microbiome	759:768	arg1	potential					738:746	functional potential	727:746	functional potential	727:746	RESULTS Using a mouse model for exposure to high LET radiation, we observed substantial changes in the composition and functional potential of the gut microbiome.
28821301	6	34	from	dynamic	980:986	arg1	composition					1015:1025	microbial and metabolic composition	991:1025	microbial and metabolic composition	991:1025	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	7	35	theme	observed	1202:1209	arg1	changes					1229:1235	The observed radiation-induced changes	1198:1235	The observed radiation-induced changes in microbiota diversity and composition	1198:1275	The observed radiation-induced changes in microbiota diversity and composition were analyzed at the functional level.
28821301	6	36	theme	transient	1071:1079	arg1	interactions					1097:1108	transient, dose-dependent interactions	1071:1108	transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes	1071:1195	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	8	37	theme	dose-dependent	1627:1640	arg1	changes					1642:1648	dose-dependent changes	1627:1648	dose-dependent changes in the abundance of specific taxa	1627:1682	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	8	38	theme	enzymatic	1414:1422	arg1	reactions					1424:1432	microbiome-specific enzymatic reactions	1394:1432	microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis	1394:1511	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	3	39	theme	gut	440:442	arg1	microbiome					444:453	the gut microbiome	436:453	the gut microbiome	436:453	Although the importance of the gut microbiome in the maintenance of human health is well established, little is known about the role of radiation in altering the microbiome during deep-space travel.
28821301	0	40	theme	Space-type	0:9	arg1	radiation					11:19	Space-type radiation	0:19	Space-type radiation	0:19	Space-type radiation induces multimodal responses in the mouse gut microbiome and metabolome.
28821301	1	41	theme	BACKGROUND	94:103	arg1	travel					111:116	BACKGROUND Space travel	94:116	BACKGROUND Space travel	94:116	BACKGROUND Space travel is associated with continuous low dose rate exposure to high linear energy transfer (LET) radiation.
28821301	3	42	theme	human	477:481	arg1	health					483:488	human health	477:488	human health	477:488	Although the importance of the gut microbiome in the maintenance of human health is well established, little is known about the role of radiation in altering the microbiome during deep-space travel.
28821301	0	43	dep	gut	63:65	arg1	metabolome					82:91	metabolome	82:91	metabolome	82:91	Space-type radiation induces multimodal responses in the mouse gut microbiome and metabolome.
28821301	0	43	dep	gut	63:65	arg1	microbiome					67:76	microbiome	67:76	microbiome	67:76	Space-type radiation induces multimodal responses in the mouse gut microbiome and metabolome.
28821301	5	44	theme	enzymatic	873:881	arg1	activity					883:890	the enzymatic activity	869:890	the enzymatic activity of the predicted metagenome	869:918	These were accompanied by changes in the abundance of multiple metabolites, which were related to the enzymatic activity of the predicted metagenome by means of metabolic network modeling.
28821301	10	45	theme	effects	2005:2011	arg1	understanding					1976:1988	our understanding	1972:1988	our understanding of the chronic effects of space radiation on human health	1972:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	6	46	theme	different	1030:1038	arg1	doses					1050:1054	different radiation doses	1030:1054	different radiation doses	1030:1054	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	8	47	theme	specific	1670:1677	arg1	taxa					1679:1682	specific taxa	1670:1682	specific taxa	1670:1682	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	9	48	theme	microbiome-mediated	1716:1734	arg1	pathophysiology					1736:1750	microbiome-mediated pathophysiology	1716:1750	microbiome-mediated pathophysiology	1716:1750	CONCLUSIONS The implication of microbiome-mediated pathophysiology after low dose ionizing radiation may be an unappreciated biologic hazard of space travel and deserves experimental validation.
28821301	8	49	theme	lipopolysaccharide	1481:1498	arg1	biosynthesis					1500:1511	lipopolysaccharide biosynthesis	1481:1511	lipopolysaccharide biosynthesis	1481:1511	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	7	50	theme	functional	1298:1307	arg1	level					1309:1313	the functional level	1294:1313	the functional level	1294:1313	The observed radiation-induced changes in microbiota diversity and composition were analyzed at the functional level.
28821301	10	51	theme	space	2016:2020	arg1	radiation					2022:2030	space radiation	2016:2030	space radiation	2016:2030	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	2	52	theme	host	387:390	arg1	expression					397:406	the microbiome and host gene expression	368:406	expression	397:406	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	5	53	from	changes	797:803	arg1	abundance					812:820	the abundance	808:820	the abundance	808:820	These were accompanied by changes in the abundance of multiple metabolites, which were related to the enzymatic activity of the predicted metagenome by means of metabolic network modeling.
28821301	10	54	theme	new	2073:2075	arg1	targets					2077:2083	potential new targets	2063:2083	potential new targets for intervention in adverse radiation effects	2063:2129	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	9	55	theme	unappreciated	1796:1808	arg1	hazard					1819:1824	an unappreciated biologic hazard	1793:1824	an unappreciated biologic hazard of space travel	1793:1840	CONCLUSIONS The implication of microbiome-mediated pathophysiology after low dose ionizing radiation may be an unappreciated biologic hazard of space travel and deserves experimental validation.
28821301	6	56	theme	damage	1173:1178	arg1	processes					1187:1195	the host's cellular damage repair processes	1153:1195	the host's cellular damage repair processes	1153:1195	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	4	57	theme	high	652:655	arg1	radiation					661:669	high LET radiation	652:669	high LET radiation	652:669	RESULTS Using a mouse model for exposure to high LET radiation, we observed substantial changes in the composition and functional potential of the gut microbiome.
28821301	1	58	theme	linear	179:184	arg1	radiation					208:216	high linear energy transfer (LET) radiation	174:216	high linear energy transfer (LET) radiation	174:216	BACKGROUND Space travel is associated with continuous low dose rate exposure to high linear energy transfer (LET) radiation.
28821301	10	59	from	health	2041:2046	arg1	understanding					1976:1988	our understanding	1972:1988	our understanding of the chronic effects of space radiation on human health	1972:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	2	60	theme	non-cytocidal	314:326	arg1	changes					357:363	changes	357:363	changes in the microbiome and host gene expression	357:406	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	2	60	theme	non-cytocidal	314:326	arg1	effects					338:344	non-cytocidal radiation effects	314:344	non-cytocidal radiation effects	314:344	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	5	61	theme	multiple	825:832	arg1	metabolites					834:844	multiple metabolites	825:844	multiple metabolites	825:844	These were accompanied by changes in the abundance of multiple metabolites, which were related to the enzymatic activity of the predicted metagenome by means of metabolic network modeling.
28821301	10	62	theme	analytical	1917:1926	arg1	basis					1928:1932	a conceptual and analytical basis	1900:1932	a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health	1900:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	9	63	theme	space	1829:1833	arg1	travel					1835:1840	space travel	1829:1840	space travel	1829:1840	CONCLUSIONS The implication of microbiome-mediated pathophysiology after low dose ionizing radiation may be an unappreciated biologic hazard of space travel and deserves experimental validation.
28821301	1	64	theme	LET	203:205	arg1	radiation					208:216	high linear energy transfer (LET) radiation	174:216	high linear energy transfer (LET) radiation	174:216	BACKGROUND Space travel is associated with continuous low dose rate exposure to high linear energy transfer (LET) radiation.
28821301	10	65	dep	investigations	1945:1958	arg1	increase					1963:1970	increase	1963:1970	to increase our understanding of the chronic effects of space radiation on human health	1960:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	10	66	theme	further	1937:1943	arg1	investigations					1945:1958	further investigations	1937:1958	further investigations to increase our understanding of the chronic effects of space radiation on human health	1937:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	5	67	theme	predicted	899:907	arg1	metagenome					909:918	the predicted metagenome	895:918	the predicted metagenome	895:918	These were accompanied by changes in the abundance of multiple metabolites, which were related to the enzymatic activity of the predicted metagenome by means of metabolic network modeling.
28821301	9	68	theme	experimental	1855:1866	arg1	validation					1868:1877	experimental validation	1855:1877	experimental validation	1855:1877	CONCLUSIONS The implication of microbiome-mediated pathophysiology after low dose ionizing radiation may be an unappreciated biologic hazard of space travel and deserves experimental validation.
28821301	8	69	theme	radiation-responsive	1542:1561	arg1	pathways					1563:1570	other radiation-responsive pathways	1536:1570	other radiation-responsive pathways like phosphatidylinositol signaling	1536:1606	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	10	70	theme	radiation	2113:2121	arg1	effects					2123:2129	adverse radiation effects	2105:2129	adverse radiation effects	2105:2129	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	2	71	theme	radiation	268:276	arg1	exposure					278:285	low dose radiation exposure	259:285	low dose radiation exposure	259:285	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	2	72	theme	low	259:261	arg1	exposure					278:285	low dose radiation exposure	259:285	low dose radiation exposure	259:285	Pathophysiological manifestations after low dose radiation exposure are strongly influenced by non-cytocidal radiation effects, including changes in the microbiome and host gene expression.
28821301	1	73	theme	transfer	193:200	arg1	radiation					208:216	high linear energy transfer (LET) radiation	174:216	high linear energy transfer (LET) radiation	174:216	BACKGROUND Space travel is associated with continuous low dose rate exposure to high linear energy transfer (LET) radiation.
28821301	1	74	theme	low	148:150	arg1	exposure					162:169	continuous low dose rate exposure	137:169	continuous low dose rate exposure to high linear energy transfer (LET) radiation	137:216	BACKGROUND Space travel is associated with continuous low dose rate exposure to high linear energy transfer (LET) radiation.
28821301	6	75	theme	metabolic	1005:1013	arg1	composition					1015:1025	microbial and metabolic composition	991:1025	microbial and metabolic composition	991:1025	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	3	76	from	importance	422:431	arg1	maintenance					462:472	the maintenance	458:472	the maintenance of human health	458:488	Although the importance of the gut microbiome in the maintenance of human health is well established, little is known about the role of radiation in altering the microbiome during deep-space travel.
28821301	10	77	from	intervention	2089:2100	arg1	effects					2123:2129	adverse radiation effects	2105:2129	adverse radiation effects	2105:2129	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	1	78	theme	rate	157:160	arg1	exposure					162:169	continuous low dose rate exposure	137:169	continuous low dose rate exposure to high linear energy transfer (LET) radiation	137:216	BACKGROUND Space travel is associated with continuous low dose rate exposure to high linear energy transfer (LET) radiation.
28821301	6	79	theme	microbial	991:999	arg1	composition					1015:1025	microbial and metabolic composition	991:1025	microbial and metabolic composition	991:1025	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	4	80	theme	substantial	684:694	arg1	changes					696:702	substantial changes	684:702	substantial changes in the composition and functional potential of the gut microbiome	684:768	RESULTS Using a mouse model for exposure to high LET radiation, we observed substantial changes in the composition and functional potential of the gut microbiome.
28821301	7	81	theme	radiation-induced	1211:1227	arg1	changes					1229:1235	The observed radiation-induced changes	1198:1235	The observed radiation-induced changes in microbiota diversity and composition	1198:1275	The observed radiation-induced changes in microbiota diversity and composition were analyzed at the functional level.
28821301	8	82	from	change	1331:1336	arg1	activity					1341:1348	activity	1341:1348	activity	1341:1348	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	9	83	dep	ionizing	1767:1774	arg1	deserves					1846:1853	deserves	1846:1853	deserves experimental validation	1846:1877	CONCLUSIONS The implication of microbiome-mediated pathophysiology after low dose ionizing radiation may be an unappreciated biologic hazard of space travel and deserves experimental validation.
28821301	9	83	dep	ionizing	1767:1774	arg1	hazard					1819:1824	an unappreciated biologic hazard	1793:1824	an unappreciated biologic hazard of space travel	1793:1840	CONCLUSIONS The implication of microbiome-mediated pathophysiology after low dose ionizing radiation may be an unappreciated biologic hazard of space travel and deserves experimental validation.
28821301	6	84	theme	dynamic	980:986	arg1	complex					972:978	a complex	970:978	a complex dynamic in microbial and metabolic composition	970:1025	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	8	85	theme	several	1364:1370	arg1	pathways					1372:1379	several pathways	1364:1379	several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis	1364:1511	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	4	86	theme	gut	755:757	arg1	microbiome					759:768	the gut microbiome	751:768	the gut microbiome	751:768	RESULTS Using a mouse model for exposure to high LET radiation, we observed substantial changes in the composition and functional potential of the gut microbiome.
28821301	8	87	theme	microbiome-specific	1394:1412	arg1	reactions					1424:1432	microbiome-specific enzymatic reactions	1394:1432	microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis	1394:1511	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	3	88	theme	microbiome	444:453	arg1	importance					422:431	the importance	418:431	the importance of the gut microbiome in the maintenance of human health	418:488	Although the importance of the gut microbiome in the maintenance of human health is well established, little is known about the role of radiation in altering the microbiome during deep-space travel.
28821301	0	89	theme	multimodal	29:38	arg1	responses					40:48	multimodal responses	29:48	multimodal responses	29:48	Space-type radiation induces multimodal responses in the mouse gut microbiome and metabolome.
28821301	9	90	theme	low	1758:1760	arg1	dose					1762:1765	low dose	1758:1765	low dose ionizing radiation may be an unappreciated biologic hazard of space travel and deserves experimental validation	1758:1877	CONCLUSIONS The implication of microbiome-mediated pathophysiology after low dose ionizing radiation may be an unappreciated biologic hazard of space travel and deserves experimental validation.
28821301	5	91	theme	network	942:948	arg1	modeling					950:957	metabolic network modeling	932:957	metabolic network modeling	932:957	These were accompanied by changes in the abundance of multiple metabolites, which were related to the enzymatic activity of the predicted metagenome by means of metabolic network modeling.
28821301	6	92	dep	transient	1071:1079	arg1	dose-dependent					1082:1095	dose-dependent	1082:1095	dose-dependent	1082:1095	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	3	93	theme	health	483:488	arg1	maintenance					462:472	the maintenance	458:472	the maintenance of human health	458:488	Although the importance of the gut microbiome in the maintenance of human health is well established, little is known about the role of radiation in altering the microbiome during deep-space travel.
28821301	8	94	from	changes	1642:1648	arg1	abundance					1657:1665	the abundance	1653:1665	the abundance of specific taxa	1653:1682	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	10	95	from	understanding	1976:1988	arg1	health					2041:2046	human health	2035:2046	human health	2035:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	6	96	theme	radiation	1040:1048	arg1	doses					1050:1054	different radiation doses	1030:1054	different radiation doses	1030:1054	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	8	97	theme	taxa	1679:1682	arg1	abundance					1657:1665	the abundance	1653:1665	the abundance of specific taxa	1653:1682	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	10	98	theme	chronic	1997:2003	arg1	effects					2005:2011	the chronic effects	1993:2011	the chronic effects of space radiation on human health	1993:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	8	99	theme	carbohydrate	1439:1450	arg1	digestion					1452:1460	carbohydrate digestion	1439:1460	carbohydrate digestion	1439:1460	A constitutive change in activity was found for several pathways dominated by microbiome-specific enzymatic reactions like carbohydrate digestion and absorption and lipopolysaccharide biosynthesis, while the activity in other radiation-responsive pathways like phosphatidylinositol signaling could be linked to dose-dependent changes in the abundance of specific taxa.
28821301	0	100	theme	mouse	57:61	arg1	gut					63:65	the mouse gut microbiome and metabolome	53:91	the mouse gut microbiome and metabolome	53:91	Space-type radiation induces multimodal responses in the mouse gut microbiome and metabolome.
28821301	10	101	theme	human	2035:2039	arg1	health					2041:2046	human health	2035:2046	human health	2035:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	9	102	theme	pathophysiology	1736:1750	arg1	implication					1701:1711	The implication	1697:1711	The implication of microbiome-mediated pathophysiology	1697:1750	CONCLUSIONS The implication of microbiome-mediated pathophysiology after low dose ionizing radiation may be an unappreciated biologic hazard of space travel and deserves experimental validation.
28821301	10	103	theme	investigations	1945:1958	arg1	basis					1928:1932	a conceptual and analytical basis	1900:1932	a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health	1900:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	10	104	theme	radiation	2022:2030	arg1	effects					2005:2011	the chronic effects	1993:2011	the chronic effects of space radiation on human health	1993:2046	This study provides a conceptual and analytical basis of further investigations to increase our understanding of the chronic effects of space radiation on human health, and points to potential new targets for intervention in adverse radiation effects.
28821301	4	105	dep	composition	711:721	arg1	the					707:709	the	707:709	the	707:709	RESULTS Using a mouse model for exposure to high LET radiation, we observed substantial changes in the composition and functional potential of the gut microbiome.
28821301	6	106	from	composition	1015:1025	arg1	dynamic					980:986	dynamic	980:986	dynamic	980:986	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	6	107	theme	microbial	1118:1126	arg1	ecology					1128:1134	microbial ecology	1118:1134	microbial ecology	1118:1134	There was a complex dynamic in microbial and metabolic composition at different radiation doses, suggestive of transient, dose-dependent interactions between microbial ecology and signals from the host's cellular damage repair processes.
28821301	4	108	theme	mouse	624:628	arg1	model					630:634	a mouse model	622:634	a mouse model for exposure to high LET radiation	622:669	RESULTS Using a mouse model for exposure to high LET radiation, we observed substantial changes in the composition and functional potential of the gut microbiome.
28821301	4	109	from	changes	696:702	arg1	composition					711:721	composition	711:721	composition	711:721	RESULTS Using a mouse model for exposure to high LET radiation, we observed substantial changes in the composition and functional potential of the gut microbiome.
28821301	4	109	from	changes	696:702	arg1	potential					738:746	functional potential	727:746	functional potential	727:746	RESULTS Using a mouse model for exposure to high LET radiation, we observed substantial changes in the composition and functional potential of the gut microbiome.
28577980	6	0	theme	polysaccharide	851:864	arg1	esterification					829:842	esterification	829:842	esterification of the polysaccharide	829:864	The degree of esterification of the polysaccharide changed drastically (from 97% to 10%) depending on the temperature used during the extraction process.
28577980	8	1	theme	methyl	1278:1283	arg1	groups					1291:1296	the methyl ester groups	1274:1296	the methyl ester groups	1274:1296	The study showed that the gelation was divalent cation-mediated and probably facilitated by the low degree of esterification which reduced steric hindrance from the methyl ester groups.
28577980	5	2	dep	Fourier	657:663	arg1	Transform					665:673	Transform	665:673	Transform Infra-Red (FTIR)	665:690	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	4	3	theme	neutral	548:554	arg1	sugars					556:561	neutral sugars	548:561	neutral sugars (∼6.8% w/w)	548:573	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	4	3	theme	neutral	548:554	arg1	%					568:568	∼6.8% w/w	564:572	∼6.8% w/w	564:572	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	4	4	theme	w/w	539:541	arg1	acid					526:529	mainly galacturonic acid	506:529	mainly galacturonic acid (∼35.8% w/w)	506:542	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	4	4	theme	w/w	539:541	arg1	%					537:537	∼35.8% w/w	532:541	∼35.8% w/w	532:541	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	0	5	from	leaf	54:57	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of pectin from green jelly leaf (Cyclea barbata Miers).	0:81	Physicochemical properties of pectin from green jelly leaf (Cyclea barbata Miers).
28577980	1	6	dep	Jelly	110:114	arg1	leaves					116:121	leaves	116:121	leaves	116:121	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	3	7	theme	w/w	391:393	arg1	calcium					376:382	calcium	376:382	calcium (∼1.2% w/w)	376:394	The mineral portion consisted of mainly calcium (∼1.2% w/w), zinc (∼0.12% w/w) and magnesium (∼0.11% w/w).
28577980	3	7	theme	w/w	391:393	arg1	%					389:389	∼1.2% w/w	385:393	∼1.2% w/w	385:393	The mineral portion consisted of mainly calcium (∼1.2% w/w), zinc (∼0.12% w/w) and magnesium (∼0.11% w/w).
28577980	4	8	theme	molecular	598:606	arg1	weight					608:613	a weight-average molecular weight	581:613	a weight-average molecular weight of ∼4.4×105g/mol	581:630	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	5	9	theme	similar	745:751	arg1	pectin					801:806	the commercial low-methoxyl pectin	773:806	the commercial low-methoxyl pectin (LMP)	773:812	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	5	9	theme	similar	745:751	arg1	fingerprint					758:768	a fairly similar FTIR fingerprint	736:768	a fairly similar FTIR fingerprint	736:768	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	8	10	from	groups	1291:1296	arg1	hindrance					1259:1267	steric hindrance	1252:1267	steric hindrance from the methyl ester groups	1252:1296	The study showed that the gelation was divalent cation-mediated and probably facilitated by the low degree of esterification which reduced steric hindrance from the methyl ester groups.
28577980	6	11	theme	esterification	829:842	arg1	degree					819:824	The degree	815:824	The degree of esterification of the polysaccharide	815:864	The degree of esterification of the polysaccharide changed drastically (from 97% to 10%) depending on the temperature used during the extraction process.
28577980	2	12	theme	w/w	330:332	arg1	%					328:328	∼6% w/w	326:332	∼6% w/w	326:332	The composition of GJL consisted mainly of carbohydrate (∼70w/w), protein (∼13% w/w) and minerals (∼6% w/w).
28577980	2	12	theme	w/w	330:332	arg1	minerals					316:323	minerals	316:323	minerals (∼6% w/w)	316:333	The composition of GJL consisted mainly of carbohydrate (∼70w/w), protein (∼13% w/w) and minerals (∼6% w/w).
28577980	8	13	theme	ester	1285:1289	arg1	groups					1291:1296	the methyl ester groups	1274:1296	the methyl ester groups	1274:1296	The study showed that the gelation was divalent cation-mediated and probably facilitated by the low degree of esterification which reduced steric hindrance from the methyl ester groups.
28577980	4	14	theme	∼4.4×105g/mol	618:630	arg1	weight					608:613	a weight-average molecular weight	581:613	a weight-average molecular weight of ∼4.4×105g/mol	581:630	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	2	15	theme	GJL	246:248	arg1	composition					231:241	The composition	227:241	The composition of GJL	227:248	The composition of GJL consisted mainly of carbohydrate (∼70w/w), protein (∼13% w/w) and minerals (∼6% w/w).
28577980	5	16	contain	had	732:734	arg1	fraction					723:730	GJL polysaccharide fraction	704:730	GJL polysaccharide fraction	704:730	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	5	16	contain	had	732:734	arg2	pectin					801:806	the commercial low-methoxyl pectin	773:806	the commercial low-methoxyl pectin (LMP)	773:812	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	5	16	contain	had	732:734	arg2	fingerprint					758:768	a fairly similar FTIR fingerprint	736:768	a fairly similar FTIR fingerprint	736:768	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	0	17	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of pectin from green jelly leaf (Cyclea barbata Miers).	0:81	Physicochemical properties of pectin from green jelly leaf (Cyclea barbata Miers).
28577980	8	18	theme	esterification	1223:1236	arg1	degree					1213:1218	the low degree	1205:1218	the low degree of esterification which reduced steric hindrance from the methyl ester groups	1205:1296	The study showed that the gelation was divalent cation-mediated and probably facilitated by the low degree of esterification which reduced steric hindrance from the methyl ester groups.
28577980	3	19	theme	w/w	410:412	arg1	zinc					397:400	zinc	397:400	zinc (∼0.12% w/w)	397:413	The mineral portion consisted of mainly calcium (∼1.2% w/w), zinc (∼0.12% w/w) and magnesium (∼0.11% w/w).
28577980	3	19	theme	w/w	410:412	arg1	%					408:408	∼0.12% w/w	403:412	∼0.12% w/w	403:412	The mineral portion consisted of mainly calcium (∼1.2% w/w), zinc (∼0.12% w/w) and magnesium (∼0.11% w/w).
28577980	7	20	theme	commercial	1068:1077	arg1	LMP					1079:1081	the commercial LMP	1064:1081	the commercial LMP	1064:1081	The zeta potential of the extracted polysaccharide showed high negative charged as compared to the commercial LMP but close to sodium alginate.
28577980	0	21	theme	pectin	30:35	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of pectin from green jelly leaf (Cyclea barbata Miers).	0:81	Physicochemical properties of pectin from green jelly leaf (Cyclea barbata Miers).
28577980	1	22	theme	room	209:212	arg1	temperature					214:224	room temperature	209:224	room temperature	209:224	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	5	23	theme	polysaccharide	708:721	arg1	fraction					723:730	GJL polysaccharide fraction	704:730	GJL polysaccharide fraction	704:730	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	5	24	theme	commercial	777:786	arg1	LMP					809:811	LMP	809:811	LMP	809:811	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	5	24	theme	commercial	777:786	arg1	pectin					801:806	the commercial low-methoxyl pectin	773:806	the commercial low-methoxyl pectin (LMP)	773:812	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	5	24	theme	commercial	777:786	arg1	fingerprint					758:768	a fairly similar FTIR fingerprint	736:768	a fairly similar FTIR fingerprint	736:768	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	5	25	theme	GJL	704:706	arg1	fraction					723:730	GJL polysaccharide fraction	704:730	GJL polysaccharide fraction	704:730	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	1	26	theme	water	87:91	arg1	extract					93:99	The water extract	83:99	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40)	83:175	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	1	26	theme	water	87:91	arg1	capable					181:187	capable	181:187	capable	181:187	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	3	27	theme	mineral	340:346	arg1	portion					348:354	The mineral portion	336:354	The mineral portion	336:354	The mineral portion consisted of mainly calcium (∼1.2% w/w), zinc (∼0.12% w/w) and magnesium (∼0.11% w/w).
28577980	0	28	theme	green	42:46	arg1	Miers					75:79	Miers	75:79	Miers	75:79	Physicochemical properties of pectin from green jelly leaf (Cyclea barbata Miers).
28577980	0	28	theme	green	42:46	arg1	leaf					54:57	green jelly leaf	42:57	green jelly leaf (Cyclea barbata Miers)	42:80	Physicochemical properties of pectin from green jelly leaf (Cyclea barbata Miers).
28577980	1	29	dep	crushing	141:148	arg1	the					150:152	the	150:152	the	150:152	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	1	29	dep	crushing	141:148	arg1	leaves					154:159	leaves	154:159	leaves	154:159	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	4	30	theme	polysaccharide	456:469	arg1	fraction					471:478	The isolated polysaccharide fraction	443:478	The isolated polysaccharide fraction (∼42.6% w/w)	443:491	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	4	30	theme	polysaccharide	456:469	arg1	%					486:486	∼42.6% w/w	481:490	∼42.6% w/w	481:490	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	8	31	theme	low	1209:1211	arg1	degree					1213:1218	the low degree	1205:1218	the low degree of esterification which reduced steric hindrance from the methyl ester groups	1205:1296	The study showed that the gelation was divalent cation-mediated and probably facilitated by the low degree of esterification which reduced steric hindrance from the methyl ester groups.
28577980	5	32	theme	low-methoxyl	788:799	arg1	LMP					809:811	LMP	809:811	LMP	809:811	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	5	32	theme	low-methoxyl	788:799	arg1	pectin					801:806	the commercial low-methoxyl pectin	773:806	the commercial low-methoxyl pectin (LMP)	773:812	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	5	32	theme	low-methoxyl	788:799	arg1	fingerprint					758:768	a fairly similar FTIR fingerprint	736:768	a fairly similar FTIR fingerprint	736:768	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	1	33	from	water	164:168	arg1	crushing					141:148	crushing	141:148	crushing	141:148	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	2	34	theme	w/w	307:309	arg1	protein					293:299	protein	293:299	protein (∼13% w/w)	293:310	The composition of GJL consisted mainly of carbohydrate (∼70w/w), protein (∼13% w/w) and minerals (∼6% w/w).
28577980	2	34	theme	w/w	307:309	arg1	%					305:305	∼13% w/w	302:309	∼13% w/w	302:309	The composition of GJL consisted mainly of carbohydrate (∼70w/w), protein (∼13% w/w) and minerals (∼6% w/w).
28577980	4	35	theme	weight-average	583:596	arg1	weight					608:613	a weight-average molecular weight	581:613	a weight-average molecular weight of ∼4.4×105g/mol	581:630	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	5	36	dep	Infra-Red	675:683	arg1	FTIR					686:689	FTIR	686:689	FTIR	686:689	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	4	37	theme	galacturonic	513:524	arg1	acid					526:529	mainly galacturonic acid	506:529	mainly galacturonic acid (∼35.8% w/w)	506:542	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	4	37	theme	galacturonic	513:524	arg1	%					537:537	∼35.8% w/w	532:541	∼35.8% w/w	532:541	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	0	38	theme	jelly	48:52	arg1	Miers					75:79	Miers	75:79	Miers	75:79	Physicochemical properties of pectin from green jelly leaf (Cyclea barbata Miers).
28577980	0	38	theme	jelly	48:52	arg1	leaf					54:57	green jelly leaf	42:57	green jelly leaf (Cyclea barbata Miers)	42:80	Physicochemical properties of pectin from green jelly leaf (Cyclea barbata Miers).
28577980	6	39	dep	changed	866:872	arg1	%					901:901	97% to 10%	892:901	97% to 10%	892:901	The degree of esterification of the polysaccharide changed drastically (from 97% to 10%) depending on the temperature used during the extraction process.
28577980	6	40	theme	97	892:893	arg1	%					894:894	%	894:894	%	894:894	The degree of esterification of the polysaccharide changed drastically (from 97% to 10%) depending on the temperature used during the extraction process.
28577980	7	41	theme	extracted	995:1003	arg1	polysaccharide					1005:1018	the extracted polysaccharide	991:1018	the extracted polysaccharide	991:1018	The zeta potential of the extracted polysaccharide showed high negative charged as compared to the commercial LMP but close to sodium alginate.
28577980	5	42	theme	FTIR	753:756	arg1	pectin					801:806	the commercial low-methoxyl pectin	773:806	the commercial low-methoxyl pectin (LMP)	773:812	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	5	42	theme	FTIR	753:756	arg1	fingerprint					758:768	a fairly similar FTIR fingerprint	736:768	a fairly similar FTIR fingerprint	736:768	The results obtained by Fourier Transform Infra-Red (FTIR) showed that GJL polysaccharide fraction had a fairly similar FTIR fingerprint as the commercial low-methoxyl pectin (LMP).
28577980	3	43	theme	w/w	437:439	arg1	magnesium					419:427	magnesium	419:427	magnesium (∼0.11% w/w)	419:440	The mineral portion consisted of mainly calcium (∼1.2% w/w), zinc (∼0.12% w/w) and magnesium (∼0.11% w/w).
28577980	3	43	theme	w/w	437:439	arg1	%					435:435	∼0.11% w/w	430:439	∼0.11% w/w	430:439	The mineral portion consisted of mainly calcium (∼1.2% w/w), zinc (∼0.12% w/w) and magnesium (∼0.11% w/w).
28577980	1	44	theme	Green	104:108	arg1	Jelly					110:114	Green Jelly leaves	104:121	Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40)	104:175	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	1	44	theme	Green	104:108	arg1	GJL					124:126	GJL	124:126	GJL	124:126	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	6	45	theme	10	899:900	arg1	%					894:894	%	894:894	%	894:894	The degree of esterification of the polysaccharide changed drastically (from 97% to 10%) depending on the temperature used during the extraction process.
28577980	6	46	theme	extraction	949:958	arg1	process					960:966	the extraction process	945:966	the extraction process	945:966	The degree of esterification of the polysaccharide changed drastically (from 97% to 10%) depending on the temperature used during the extraction process.
28577980	7	47	theme	sodium	1096:1101	arg1	alginate					1103:1110	sodium alginate	1096:1110	sodium alginate	1096:1110	The zeta potential of the extracted polysaccharide showed high negative charged as compared to the commercial LMP but close to sodium alginate.
28577980	1	48	from	crushing	141:148	arg1	1:40					171:174	1:40	171:174	1:40	171:174	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	1	48	from	crushing	141:148	arg1	water					164:168	water	164:168	water (1:40)	164:175	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	1	49	theme	Jelly	110:114	arg1	extract					93:99	The water extract	83:99	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40)	83:175	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	1	49	theme	Jelly	110:114	arg1	capable					181:187	capable	181:187	capable	181:187	The water extract of Green Jelly leaves (GJL) obtained by crushing the leaves in water (1:40) was capable of forming a gel at room temperature.
28577980	7	50	theme	polysaccharide	1005:1018	arg1	potential					978:986	The zeta potential	969:986	The zeta potential of the extracted polysaccharide	969:1018	The zeta potential of the extracted polysaccharide showed high negative charged as compared to the commercial LMP but close to sodium alginate.
28577980	4	51	theme	isolated	447:454	arg1	fraction					471:478	The isolated polysaccharide fraction	443:478	The isolated polysaccharide fraction (∼42.6% w/w)	443:491	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	4	51	theme	isolated	447:454	arg1	%					486:486	∼42.6% w/w	481:490	∼42.6% w/w	481:490	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	6	52	dep	%	894:894	arg1	to					896:897	to	896:897	to	896:897	The degree of esterification of the polysaccharide changed drastically (from 97% to 10%) depending on the temperature used during the extraction process.
28577980	7	53	theme	zeta	973:976	arg1	potential					978:986	The zeta potential	969:986	The zeta potential of the extracted polysaccharide	969:1018	The zeta potential of the extracted polysaccharide showed high negative charged as compared to the commercial LMP but close to sodium alginate.
28577980	8	54	theme	steric	1252:1257	arg1	hindrance					1259:1267	steric hindrance	1252:1267	steric hindrance from the methyl ester groups	1252:1296	The study showed that the gelation was divalent cation-mediated and probably facilitated by the low degree of esterification which reduced steric hindrance from the methyl ester groups.
28577980	4	55	theme	w/w	570:572	arg1	sugars					556:561	neutral sugars	548:561	neutral sugars (∼6.8% w/w)	548:573	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	4	55	theme	w/w	570:572	arg1	%					568:568	∼6.8% w/w	564:572	∼6.8% w/w	564:572	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	4	56	theme	w/w	488:490	arg1	fraction					471:478	The isolated polysaccharide fraction	443:478	The isolated polysaccharide fraction (∼42.6% w/w)	443:491	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
28577980	4	56	theme	w/w	488:490	arg1	%					486:486	∼42.6% w/w	481:490	∼42.6% w/w	481:490	The isolated polysaccharide fraction (∼42.6% w/w) consisted of mainly galacturonic acid (∼35.8% w/w) and neutral sugars (∼6.8% w/w), with a weight-average molecular weight of ∼4.4×105g/mol.
27155797	7	0	theme	volatiles	1039:1047	arg1	types					1030:1034	the detailed types	1017:1034	the detailed types of volatiles and product distribution	1017:1072	Finally, the detailed types of volatiles and product distribution were measured by Py-GC-MS.
27155797	0	1	theme	polyhydric	71:80	arg1	alcohols					82:89	polyhydric alcohols	71:89	polyhydric alcohols	71:89	Kinetic and product composition study on the cellulose liquefaction in polyhydric alcohols.
27155797	6	2	theme	mechanism	802:810	arg1	functions					812:820	mechanism functions	802:820	mechanism functions	802:820	The kinetic parameters and mechanism functions between cellulose liquefaction in PEG400 and in glycerol were quite different, which verified that solvolysis behavior and reaction process were seriously influenced by solvent species.
27155797	6	3	from	parameters	787:796	arg1	PEG400					856:861	PEG400	856:861	PEG400	856:861	The kinetic parameters and mechanism functions between cellulose liquefaction in PEG400 and in glycerol were quite different, which verified that solvolysis behavior and reaction process were seriously influenced by solvent species.
27155797	6	3	from	parameters	787:796	arg1	glycerol					870:877	glycerol	870:877	glycerol	870:877	The kinetic parameters and mechanism functions between cellulose liquefaction in PEG400 and in glycerol were quite different, which verified that solvolysis behavior and reaction process were seriously influenced by solvent species.
27155797	2	4	theme	liquefaction	270:281	arg1	stage					283:287	the main liquefaction stage	261:287	the main liquefaction stage	261:287	Three stages were observed during the solvolysis process and the main liquefaction stage could be further divided into two zones.
27155797	6	5	theme	solvent	991:997	arg1	species					999:1005	solvent species	991:1005	solvent species	991:1005	The kinetic parameters and mechanism functions between cellulose liquefaction in PEG400 and in glycerol were quite different, which verified that solvolysis behavior and reaction process were seriously influenced by solvent species.
27155797	2	6	theme	main	265:268	arg1	stage					283:287	the main liquefaction stage	261:287	the main liquefaction stage	261:287	Three stages were observed during the solvolysis process and the main liquefaction stage could be further divided into two zones.
27155797	6	7	theme	kinetic	779:785	arg1	parameters					787:796	The kinetic parameters	775:796	The kinetic parameters	775:796	The kinetic parameters and mechanism functions between cellulose liquefaction in PEG400 and in glycerol were quite different, which verified that solvolysis behavior and reaction process were seriously influenced by solvent species.
27155797	6	7	theme	kinetic	779:785	arg1	different					890:898	different	890:898	different	890:898	The kinetic parameters and mechanism functions between cellulose liquefaction in PEG400 and in glycerol were quite different, which verified that solvolysis behavior and reaction process were seriously influenced by solvent species.
27155797	4	8	theme	stage	648:652	arg1	analysis					614:621	the kinetic and mechanism analysis	588:621	the kinetic and mechanism analysis of the main liquefaction stage	588:652	A step-wise procedure based on iso-conversional and Master-plots methods was used for the kinetic and mechanism analysis of the main liquefaction stage.
27155797	5	9	dep	results	671:677	arg1	based					679:683	based	679:683	results based on the kinetic model were in agreement with the experimental data of the conversion rate	671:772	The calculation results based on the kinetic model were in agreement with the experimental data of the conversion rate.
27155797	4	10	dep	iso-conversional	533:548	arg1	methods					567:573	methods	567:573	methods	567:573	A step-wise procedure based on iso-conversional and Master-plots methods was used for the kinetic and mechanism analysis of the main liquefaction stage.
27155797	4	11	theme	liquefaction	635:646	arg1	stage					648:652	the main liquefaction stage	626:652	the main liquefaction stage	626:652	A step-wise procedure based on iso-conversional and Master-plots methods was used for the kinetic and mechanism analysis of the main liquefaction stage.
27155797	3	12	theme	cellulose	374:382	arg1	behavior					362:369	liquefaction behavior	349:369	liquefaction behavior of cellulose	349:382	The differences of liquefaction behavior of cellulose in the two solvents were compared, and the functional groups of volatiles produced by solvolysis were also evaluated.
27155797	3	13	theme	liquefaction	349:360	arg1	behavior					362:369	liquefaction behavior	349:369	liquefaction behavior of cellulose	349:382	The differences of liquefaction behavior of cellulose in the two solvents were compared, and the functional groups of volatiles produced by solvolysis were also evaluated.
27155797	1	14	dep	alcohols	144:151	arg1	PEG					154:156	PEG 400	154:160	PEG 400	154:160	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol) was studied by TG-FTIR.
27155797	1	14	dep	alcohols	144:151	arg1	glycerol					166:173	glycerol	166:173	glycerol	166:173	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol) was studied by TG-FTIR.
27155797	1	14	dep	alcohols	144:151	arg1	alcohols					144:151	polyhydric alcohols	133:151	polyhydric alcohols (PEG 400 and glycerol)	133:174	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol) was studied by TG-FTIR.
27155797	3	15	theme	behavior	362:369	arg1	differences					334:344	The differences	330:344	The differences of liquefaction behavior of cellulose in the two solvents	330:402	The differences of liquefaction behavior of cellulose in the two solvents were compared, and the functional groups of volatiles produced by solvolysis were also evaluated.
27155797	5	16	theme	kinetic	692:698	arg1	model					700:704	the kinetic model	688:704	the kinetic model were in agreement with the experimental data of the conversion rate	688:772	The calculation results based on the kinetic model were in agreement with the experimental data of the conversion rate.
27155797	5	17	theme	conversion	758:767	arg1	rate					769:772	the conversion rate	754:772	the conversion rate	754:772	The calculation results based on the kinetic model were in agreement with the experimental data of the conversion rate.
27155797	7	18	theme	product	1053:1059	arg1	distribution					1061:1072	product distribution	1053:1072	product distribution	1053:1072	Finally, the detailed types of volatiles and product distribution were measured by Py-GC-MS.
27155797	1	19	theme	liquefaction	96:107	arg1	process					109:115	The liquefaction process	92:115	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol)	92:174	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol) was studied by TG-FTIR.
27155797	4	20	theme	main	630:633	arg1	stage					648:652	the main liquefaction stage	626:652	the main liquefaction stage	626:652	A step-wise procedure based on iso-conversional and Master-plots methods was used for the kinetic and mechanism analysis of the main liquefaction stage.
27155797	5	21	theme	rate	769:772	arg1	data					746:749	the experimental data	729:749	the experimental data of the conversion rate	729:772	The calculation results based on the kinetic model were in agreement with the experimental data of the conversion rate.
27155797	4	22	theme	step-wise	504:512	arg1	procedure					514:522	A step-wise procedure	502:522	A step-wise procedure based on iso-conversional and Master-plots methods	502:573	A step-wise procedure based on iso-conversional and Master-plots methods was used for the kinetic and mechanism analysis of the main liquefaction stage.
27155797	6	23	from	functions	812:820	arg1	PEG400					856:861	PEG400	856:861	PEG400	856:861	The kinetic parameters and mechanism functions between cellulose liquefaction in PEG400 and in glycerol were quite different, which verified that solvolysis behavior and reaction process were seriously influenced by solvent species.
27155797	6	23	from	functions	812:820	arg1	glycerol					870:877	glycerol	870:877	glycerol	870:877	The kinetic parameters and mechanism functions between cellulose liquefaction in PEG400 and in glycerol were quite different, which verified that solvolysis behavior and reaction process were seriously influenced by solvent species.
27155797	3	24	from	differences	334:344	arg1	solvents					395:402	the two solvents	387:402	the two solvents	387:402	The differences of liquefaction behavior of cellulose in the two solvents were compared, and the functional groups of volatiles produced by solvolysis were also evaluated.
27155797	3	25	theme	functional	427:436	arg1	volatiles					448:456	volatiles	448:456	volatiles produced by solvolysis	448:479	The differences of liquefaction behavior of cellulose in the two solvents were compared, and the functional groups of volatiles produced by solvolysis were also evaluated.
27155797	3	25	theme	functional	427:436	arg1	groups					438:443	the functional groups	423:443	the functional groups of volatiles produced by solvolysis	423:479	The differences of liquefaction behavior of cellulose in the two solvents were compared, and the functional groups of volatiles produced by solvolysis were also evaluated.
27155797	0	26	theme	cellulose	45:53	arg1	liquefaction					55:66	the cellulose liquefaction	41:66	the cellulose liquefaction in polyhydric alcohols	41:89	Kinetic and product composition study on the cellulose liquefaction in polyhydric alcohols.
27155797	6	27	theme	reaction	945:952	arg1	process					954:960	reaction process	945:960	reaction process	945:960	The kinetic parameters and mechanism functions between cellulose liquefaction in PEG400 and in glycerol were quite different, which verified that solvolysis behavior and reaction process were seriously influenced by solvent species.
27155797	4	28	used	used	579:582	arg2	procedure					514:522	A step-wise procedure	502:522	A step-wise procedure based on iso-conversional and Master-plots methods	502:573	A step-wise procedure based on iso-conversional and Master-plots methods was used for the kinetic and mechanism analysis of the main liquefaction stage.
27155797	0	29	from	liquefaction	55:66	arg1	alcohols					82:89	polyhydric alcohols	71:89	polyhydric alcohols	71:89	Kinetic and product composition study on the cellulose liquefaction in polyhydric alcohols.
27155797	7	30	theme	detailed	1021:1028	arg1	types					1030:1034	the detailed types	1017:1034	the detailed types of volatiles and product distribution	1017:1072	Finally, the detailed types of volatiles and product distribution were measured by Py-GC-MS.
27155797	7	31	theme	distribution	1061:1072	arg1	types					1030:1034	the detailed types	1017:1034	the detailed types of volatiles and product distribution	1017:1072	Finally, the detailed types of volatiles and product distribution were measured by Py-GC-MS.
27155797	5	32	with	agreement	714:722	arg1	data					746:749	the experimental data	729:749	the experimental data of the conversion rate	729:772	The calculation results based on the kinetic model were in agreement with the experimental data of the conversion rate.
27155797	1	33	theme	cellulose	120:128	arg1	process					109:115	The liquefaction process	92:115	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol)	92:174	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol) was studied by TG-FTIR.
27155797	6	34	theme	solvolysis	921:930	arg1	behavior					932:939	solvolysis behavior	921:939	solvolysis behavior	921:939	The kinetic parameters and mechanism functions between cellulose liquefaction in PEG400 and in glycerol were quite different, which verified that solvolysis behavior and reaction process were seriously influenced by solvent species.
27155797	6	35	theme	cellulose	830:838	arg1	liquefaction					840:851	cellulose liquefaction	830:851	cellulose liquefaction	830:851	The kinetic parameters and mechanism functions between cellulose liquefaction in PEG400 and in glycerol were quite different, which verified that solvolysis behavior and reaction process were seriously influenced by solvent species.
27155797	3	36	theme	volatiles	448:456	arg1	volatiles					448:456	volatiles	448:456	volatiles produced by solvolysis	448:479	The differences of liquefaction behavior of cellulose in the two solvents were compared, and the functional groups of volatiles produced by solvolysis were also evaluated.
27155797	3	36	theme	volatiles	448:456	arg1	groups					438:443	the functional groups	423:443	the functional groups of volatiles produced by solvolysis	423:479	The differences of liquefaction behavior of cellulose in the two solvents were compared, and the functional groups of volatiles produced by solvolysis were also evaluated.
27155797	1	37	from	process	109:115	arg1	PEG					154:156	PEG 400	154:160	PEG 400	154:160	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol) was studied by TG-FTIR.
27155797	1	37	from	process	109:115	arg1	glycerol					166:173	glycerol	166:173	glycerol	166:173	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol) was studied by TG-FTIR.
27155797	1	37	from	process	109:115	arg1	alcohols					144:151	polyhydric alcohols	133:151	polyhydric alcohols (PEG 400 and glycerol)	133:174	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol) was studied by TG-FTIR.
27155797	4	38	theme	kinetic	592:598	arg1	analysis					614:621	the kinetic and mechanism analysis	588:621	the kinetic and mechanism analysis of the main liquefaction stage	588:652	A step-wise procedure based on iso-conversional and Master-plots methods was used for the kinetic and mechanism analysis of the main liquefaction stage.
27155797	2	39	theme	solvolysis	238:247	arg1	process					249:255	the solvolysis process	234:255	the solvolysis process	234:255	Three stages were observed during the solvolysis process and the main liquefaction stage could be further divided into two zones.
27155797	1	40	theme	polyhydric	133:142	arg1	PEG					154:156	PEG 400	154:160	PEG 400	154:160	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol) was studied by TG-FTIR.
27155797	1	40	theme	polyhydric	133:142	arg1	glycerol					166:173	glycerol	166:173	glycerol	166:173	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol) was studied by TG-FTIR.
27155797	1	40	theme	polyhydric	133:142	arg1	alcohols					144:151	polyhydric alcohols	133:151	polyhydric alcohols (PEG 400 and glycerol)	133:174	The liquefaction process of cellulose in polyhydric alcohols (PEG 400 and glycerol) was studied by TG-FTIR.
27155797	5	41	theme	experimental	733:744	arg1	data					746:749	the experimental data	729:749	the experimental data of the conversion rate	729:772	The calculation results based on the kinetic model were in agreement with the experimental data of the conversion rate.
27155797	4	42	theme	mechanism	604:612	arg1	analysis					614:621	the kinetic and mechanism analysis	588:621	the kinetic and mechanism analysis of the main liquefaction stage	588:652	A step-wise procedure based on iso-conversional and Master-plots methods was used for the kinetic and mechanism analysis of the main liquefaction stage.
27155797	0	43	theme	product	12:18	arg1	composition					20:30	product composition	12:30	product composition	12:30	Kinetic and product composition study on the cellulose liquefaction in polyhydric alcohols.
26877017	4	0	theme	chemical	823:830	arg1	composition					832:842	chemical composition	823:842	chemical composition	823:842	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	5	1	theme	nanoparticles	956:968	arg1	dependent					986:994	dependent	986:994	dependent	986:994	The morphology of the ensuing Cu2O nanoparticles was shown to be dependent on the reduycing agent used.
26877017	5	1	theme	nanoparticles	956:968	arg1	morphology					925:934	The morphology	921:934	The morphology of the ensuing Cu2O nanoparticles	921:968	The morphology of the ensuing Cu2O nanoparticles was shown to be dependent on the reduycing agent used.
26877017	2	2	theme	surface	275:281	arg1	oxidation					283:291	a mild surface oxidation	268:291	a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination	268:446	The method is based on a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination.
26877017	4	3	theme	nanoparticles	891:903	arg1	morphology					811:820	morphology	811:820	morphology	811:820	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	3	theme	nanoparticles	891:903	arg1	composition					832:842	chemical composition	823:842	chemical composition	823:842	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	3	theme	nanoparticles	891:903	arg1	size					805:808	size	805:808	size	805:808	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	3	theme	nanoparticles	891:903	arg1	structure					864:872	the crystalline structure	848:872	the crystalline structure of the generated nanoparticles	848:903	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	3	4	dep	fibres	542:547	arg1	solution					564:571	a aqueous solution	554:571	a aqueous solution of a reducing agent	554:591	Then, the adsorbed Cu(2+) ions were readly converted into Cu2O by dipping the treated cotton fibres into a aqueous solution of a reducing agent.
26877017	2	5	theme	mild	270:273	arg1	oxidation					283:291	a mild surface oxidation	268:291	a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination	268:446	The method is based on a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination.
26877017	3	6	theme	aqueous	556:562	arg1	solution					564:571	a aqueous solution	554:571	a aqueous solution of a reducing agent	554:591	Then, the adsorbed Cu(2+) ions were readly converted into Cu2O by dipping the treated cotton fibres into a aqueous solution of a reducing agent.
26877017	6	7	theme	Antibacterial	1025:1037	arg1	properties					1039:1048	Antibacterial properties	1025:1048	Antibacterial properties of the modified fibres	1025:1071	Antibacterial properties of the modified fibres were also investigated.
26877017	4	8	theme	electron	618:625	arg1	XRD					710:712	XRD	710:712	XRD	710:712	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	8	theme	electron	618:625	arg1	FE-SEM					639:644	FE-SEM	639:644	FE-SEM	639:644	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	8	theme	electron	618:625	arg1	microscopy					627:636	Field-emission scanning electron microscopy	594:636	Field-emission scanning electron microscopy (FE-SEM)	594:645	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	1	9	theme	room	202:205	arg1	temperature					207:217	room temperature	202:217	room temperature using water as a solvent	202:242	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
26877017	4	10	theme	scanning	609:616	arg1	XRD					710:712	XRD	710:712	XRD	710:712	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	10	theme	scanning	609:616	arg1	FE-SEM					639:644	FE-SEM	639:644	FE-SEM	639:644	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	10	theme	scanning	609:616	arg1	microscopy					627:636	Field-emission scanning electron microscopy	594:636	Field-emission scanning electron microscopy (FE-SEM)	594:645	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	3	11	theme	agent	587:591	arg1	solution					564:571	a aqueous solution	554:571	a aqueous solution of a reducing agent	554:591	Then, the adsorbed Cu(2+) ions were readly converted into Cu2O by dipping the treated cotton fibres into a aqueous solution of a reducing agent.
26877017	5	12	theme	ensuing	943:949	arg1	nanoparticles					956:968	the ensuing Cu2O nanoparticles	939:968	the ensuing Cu2O nanoparticles	939:968	The morphology of the ensuing Cu2O nanoparticles was shown to be dependent on the reduycing agent used.
26877017	2	13	theme	controlled	330:339	arg1	way					341:343	a controlled way	328:343	a controlled way	328:343	The method is based on a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination.
26877017	1	14	theme	nanoparticles	136:148	arg1	generation					122:131	in situ generation	114:131	in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres	114:197	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
26877017	5	15	theme	Cu2O	951:954	arg1	nanoparticles					956:968	the ensuing Cu2O nanoparticles	939:968	the ensuing Cu2O nanoparticles	939:968	The morphology of the ensuing Cu2O nanoparticles was shown to be dependent on the reduycing agent used.
26877017	4	16	theme	diffraction	697:707	arg1	methods					772:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	1	17	theme	green	66:70	arg1	procedure					87:95	A green, safe and fast procedure	64:95	A green, safe and fast procedure	64:95	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
26877017	3	18	theme	reducing	578:585	arg1	agent					587:591	a reducing agent	576:591	a reducing agent	576:591	Then, the adsorbed Cu(2+) ions were readly converted into Cu2O by dipping the treated cotton fibres into a aqueous solution of a reducing agent.
26877017	3	19	theme	adsorbed	459:466	arg1	ions					475:478	the adsorbed Cu(2+) ions	455:478	the adsorbed Cu(2+) ions	455:478	Then, the adsorbed Cu(2+) ions were readly converted into Cu2O by dipping the treated cotton fibres into a aqueous solution of a reducing agent.
26877017	2	20	theme	fibres	306:311	arg1	oxidation					283:291	a mild surface oxidation	268:291	a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination	268:446	The method is based on a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination.
26877017	0	21	theme	Controlled	0:9	arg1	growth					11:16	Controlled growth	0:16	Controlled growth of Cu2O nanoparticles	0:38	Controlled growth of Cu2O nanoparticles bound to cotton fibres.
26877017	4	22	from	size	805:808	arg1	fabrics					912:918	the fabrics	908:918	the fabrics	908:918	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	23	theme	crystalline	852:862	arg1	structure					864:872	the crystalline structure	848:872	the crystalline structure of the generated nanoparticles	848:903	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	1	24	theme	safe	73:76	arg1	procedure					87:95	A green, safe and fast procedure	64:95	A green, safe and fast procedure	64:95	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
26877017	2	25	theme	cellulose	296:304	arg1	fibres					306:311	cellulose fibres	296:311	cellulose fibres	296:311	The method is based on a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination.
26877017	0	26	theme	Cu2O	21:24	arg1	nanoparticles					26:38	Cu2O nanoparticles	21:38	Cu2O nanoparticles	21:38	Controlled growth of Cu2O nanoparticles bound to cotton fibres.
26877017	4	27	theme	Field-emission	594:607	arg1	XRD					710:712	XRD	710:712	XRD	710:712	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	27	theme	Field-emission	594:607	arg1	FE-SEM					639:644	FE-SEM	639:644	FE-SEM	639:644	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	27	theme	Field-emission	594:607	arg1	microscopy					627:636	Field-emission scanning electron microscopy	594:636	Field-emission scanning electron microscopy (FE-SEM)	594:645	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	1	28	dep	in	114:115	arg1	situ					117:120	situ	117:120	situ	117:120	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
26877017	2	29	theme	carboxylic	345:354	arg1	groups					356:361	carboxylic groups	345:361	carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination	345:446	The method is based on a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination.
26877017	4	30	theme	spectroscopic	758:770	arg1	methods					772:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	1	31	theme	fast	82:85	arg1	procedure					87:95	A green, safe and fast procedure	64:95	A green, safe and fast procedure	64:95	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
26877017	1	32	theme	cuprous	159:165	arg1	Cu2O					174:177	Cu2O	174:177	Cu2O	174:177	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
26877017	1	32	theme	cuprous	159:165	arg1	oxide					167:171	cuprous oxide	159:171	cuprous oxide (Cu2O)	159:178	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
26877017	4	33	from	structure	864:872	arg1	fabrics					912:918	the fabrics	908:918	the fabrics	908:918	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	1	34	theme	oxide	167:171	arg1	NPs					151:153	NPs	151:153	NPs	151:153	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
26877017	1	34	theme	oxide	167:171	arg1	nanoparticles					136:148	nanoparticles	136:148	nanoparticles (NPs) of cuprous oxide (Cu2O)	136:178	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
26877017	0	35	theme	nanoparticles	26:38	arg1	growth					11:16	Controlled growth	0:16	Controlled growth of Cu2O nanoparticles	0:38	Controlled growth of Cu2O nanoparticles bound to cotton fibres.
26877017	2	36	theme	electrostatic	421:433	arg1	coordination					435:446	electrostatic coordination	421:446	electrostatic coordination	421:446	The method is based on a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination.
26877017	4	37	theme	generated	881:889	arg1	nanoparticles					891:903	the generated nanoparticles	877:903	the generated nanoparticles	877:903	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	5	38	theme	reduycing	1003:1011	arg1	agent					1013:1017	the reduycing agent	999:1017	the reduycing agent used	999:1022	The morphology of the ensuing Cu2O nanoparticles was shown to be dependent on the reduycing agent used.
26877017	2	39	theme	binding	375:381	arg1	site					383:386	a binding site	373:386	a binding site for the adsorption of Cu(2+) via electrostatic coordination	373:446	The method is based on a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination.
26877017	4	40	theme	absorption	734:743	arg1	methods					772:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	41	theme	X-ray	691:695	arg1	diffraction					697:707	X-ray diffraction	691:707	X-ray diffraction	691:707	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	42	theme	UV-vis	727:732	arg1	absorption					734:743	UV-vis absorption	727:743	UV-vis absorption	727:743	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	6	43	theme	fibres	1066:1071	arg1	properties					1039:1048	Antibacterial properties	1025:1048	Antibacterial properties of the modified fibres	1025:1071	Antibacterial properties of the modified fibres were also investigated.
26877017	4	44	from	composition	832:842	arg1	fabrics					912:918	the fabrics	908:918	the fabrics	908:918	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	45	theme	emission	749:756	arg1	methods					772:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	6	46	theme	modified	1057:1064	arg1	fibres					1066:1071	the modified fibres	1053:1071	the modified fibres	1053:1071	Antibacterial properties of the modified fibres were also investigated.
26877017	4	47	theme	photoelectron	654:666	arg1	spectroscopy					668:679	X-ray photoelectron spectroscopy	648:679	X-ray photoelectron spectroscopy (XPS)	648:685	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	47	theme	photoelectron	654:666	arg1	XPS					682:684	XPS	682:684	XPS	682:684	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	48	theme	microscopy	627:636	arg1	methods					772:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	3	49	theme	treated	527:533	arg1	fibres					542:547	the treated cotton fibres	523:547	the treated cotton fibres	523:547	Then, the adsorbed Cu(2+) ions were readly converted into Cu2O by dipping the treated cotton fibres into a aqueous solution of a reducing agent.
26877017	4	50	theme	X-ray	648:652	arg1	spectroscopy					668:679	X-ray photoelectron spectroscopy	648:679	X-ray photoelectron spectroscopy (XPS)	648:685	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	50	theme	X-ray	648:652	arg1	XPS					682:684	XPS	682:684	XPS	682:684	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	4	51	used	used	785:788	arg2	methods					772:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	2	52	theme	Cu	410:411	arg1	adsorption					396:405	the adsorption	392:405	the adsorption of Cu(2+) via electrostatic coordination	392:446	The method is based on a mild surface oxidation of cellulose fibres to generate in a controlled way carboxylic groups acting as a binding site for the adsorption of Cu(2+) via electrostatic coordination.
26877017	3	53	theme	cotton	535:540	arg1	fibres					542:547	the treated cotton fibres	523:547	the treated cotton fibres	523:547	Then, the adsorbed Cu(2+) ions were readly converted into Cu2O by dipping the treated cotton fibres into a aqueous solution of a reducing agent.
26877017	1	54	theme	in	114:115	arg1	generation					122:131	in situ generation	114:131	in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres	114:197	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
26877017	4	55	from	morphology	811:820	arg1	fabrics					912:918	the fabrics	908:918	the fabrics	908:918	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	0	56	theme	cotton	49:54	arg1	fibres					56:61	cotton fibres	49:61	cotton fibres	49:61	Controlled growth of Cu2O nanoparticles bound to cotton fibres.
26877017	4	57	theme	spectroscopy	668:679	arg1	methods					772:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods	594:778	Field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), as well as UV-vis absorption and emission spectroscopic methods were used to analyse the size, morphology, chemical composition and the crystalline structure of the generated nanoparticles on the fabrics.
26877017	3	58	theme	Cu	468:469	arg1	ions					475:478	the adsorbed Cu(2+) ions	455:478	the adsorbed Cu(2+) ions	455:478	Then, the adsorbed Cu(2+) ions were readly converted into Cu2O by dipping the treated cotton fibres into a aqueous solution of a reducing agent.
26877017	1	59	theme	cotton	185:190	arg1	fibres					192:197	cotton fibres	185:197	cotton fibres	185:197	A green, safe and fast procedure is presented for in situ generation of nanoparticles (NPs) of cuprous oxide (Cu2O) onto cotton fibres at room temperature using water as a solvent.
24799212	0	0	theme	major	75:79	arg1	Pagrosomus					64:73	Pagrosomus major	64:79	Pagrosomus major	64:79	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	0	0	theme	major	75:79	arg1	bream					57:61	red sea bream	49:61	red sea bream (Pagrosomus major)	49:80	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	1	1	theme	sea	310:312	arg1	bream					314:318	red sea bream	306:318	red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days	306:368	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	1	1	theme	sea	310:312	arg1	Pagrosomus					321:330	Pagrosomus major	321:336	Pagrosomus major	321:336	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	3	2	theme	microbial	784:792	arg1	growth					794:799	reduced microbial growth compare	776:807	reduced microbial growth compare	776:807	The results indicate that treatment with gum arabic and ergothioneine (GAER) retarded nucleotide breakdown, lipid oxidation, protein degradation, and reduced microbial growth compare with the control.
24799212	1	3	theme	ergothioneine	223:235	arg1	effects					184:190	The combined effects	171:190	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days	171:368	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	5	4	theme	storage	1004:1010	arg1	period					1012:1017	the storage period	1000:1017	the storage period	1000:1017	Sensory evaluation proved the efficacy of GAER coating by maintaining the overall quality of red sea bream during the storage period.
24799212	1	5	theme	bream	314:318	arg1	characteristics					287:301	the sensory and physicochemical characteristics	255:301	the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days	255:368	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	2	6	theme	TCA-soluble	502:512	arg1	peptides					514:521	TCA-soluble peptides	502:521	TCA-soluble peptides	502:521	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	0	7	dep	coating	96:102	arg1	enriched					104:111	enriched	104:111	arabic coating enriched with ergothioneine treatment during refrigerated storage	89:168	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	2	8	theme	basic	442:446	arg1	nitrogen					448:455	total volatile basic nitrogen	427:455	total volatile basic nitrogen (TVB-N)	427:463	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	2	8	theme	basic	442:446	arg1	TVB-N					458:462	TVB-N	458:462	TVB-N	458:462	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	7	9	contain	has	1132:1134	arg2	potential					1136:1144	potential	1136:1144	potential to improve the quality of red sea bream and extend its storage life	1136:1212	Our study suggests that GAER treatment has potential to improve the quality of red sea bream and extend its storage life.
24799212	7	9	contain	has	1132:1134	arg1	treatment					1122:1130	GAER treatment	1117:1130	GAER treatment	1117:1130	Our study suggests that GAER treatment has potential to improve the quality of red sea bream and extend its storage life.
24799212	6	10	theme	textural	1067:1074	arg1	characteristics					1076:1090	textural characteristics	1067:1090	textural characteristics	1067:1090	Furthermore, GAER maintained better colour and textural characteristics.
24799212	3	11	theme	nucleotide	712:721	arg1	breakdown					723:731	nucleotide breakdown	712:731	nucleotide breakdown	712:731	The results indicate that treatment with gum arabic and ergothioneine (GAER) retarded nucleotide breakdown, lipid oxidation, protein degradation, and reduced microbial growth compare with the control.
24799212	5	12	theme	red	979:981	arg1	bream					987:991	red sea bream	979:991	red sea bream	979:991	Sensory evaluation proved the efficacy of GAER coating by maintaining the overall quality of red sea bream during the storage period.
24799212	1	13	theme	major	332:336	arg1	bream					314:318	red sea bream	306:318	red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days	306:368	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	1	13	theme	major	332:336	arg1	Pagrosomus					321:330	Pagrosomus major	321:336	Pagrosomus major	321:336	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	2	14	theme	texture	532:538	arg1	TPA					558:560	TPA	558:560	TPA	558:560	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	2	14	theme	texture	532:538	arg1	analyses					548:555	texture profile analyses	532:555	texture profile analyses (TPA)	532:561	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	2	15	theme	profile	540:546	arg1	TPA					558:560	TPA	558:560	TPA	558:560	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	2	15	theme	profile	540:546	arg1	analyses					548:555	texture profile analyses	532:555	texture profile analyses (TPA)	532:561	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	2	16	theme	proximate	394:402	arg1	composition					404:414	Fish proximate composition	389:414	Fish proximate composition	389:414	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	5	17	theme	bream	987:991	arg1	quality					968:974	the overall quality	956:974	the overall quality of red sea bream	956:991	Sensory evaluation proved the efficacy of GAER coating by maintaining the overall quality of red sea bream during the storage period.
24799212	2	18	theme	Fish	389:392	arg1	composition					404:414	Fish proximate composition	389:414	Fish proximate composition	389:414	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	7	19	theme	sea	1176:1178	arg1	bream					1180:1184	red sea bream	1172:1184	red sea bream	1172:1184	Our study suggests that GAER treatment has potential to improve the quality of red sea bream and extend its storage life.
24799212	2	20	theme	microbiological	564:578	arg1	properties					580:589	microbiological properties	564:589	microbiological properties	564:589	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	5	21	theme	sea	983:985	arg1	bream					987:991	red sea bream	979:991	red sea bream	979:991	Sensory evaluation proved the efficacy of GAER coating by maintaining the overall quality of red sea bream during the storage period.
24799212	1	22	theme	combined	175:182	arg1	effects					184:190	The combined effects	171:190	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days	171:368	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	0	23	theme	Physicochemical	0:14	arg1	responses					16:24	Physicochemical responses	0:24	Physicochemical responses	0:24	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	6	24	dep	better	1049:1054	arg1	colour					1056:1061	colour	1056:1061	colour	1056:1061	Furthermore, GAER maintained better colour and textural characteristics.
24799212	6	24	dep	better	1049:1054	arg1	characteristics					1076:1090	textural characteristics	1067:1090	textural characteristics	1067:1090	Furthermore, GAER maintained better colour and textural characteristics.
24799212	3	25	with	treatment	652:660	arg1	arabic					671:676	gum arabic	667:676	gum arabic	667:676	The results indicate that treatment with gum arabic and ergothioneine (GAER) retarded nucleotide breakdown, lipid oxidation, protein degradation, and reduced microbial growth compare with the control.
24799212	3	25	with	treatment	652:660	arg1	GAER					697:700	GAER	697:700	GAER	697:700	The results indicate that treatment with gum arabic and ergothioneine (GAER) retarded nucleotide breakdown, lipid oxidation, protein degradation, and reduced microbial growth compare with the control.
24799212	3	25	with	treatment	652:660	arg1	ergothioneine					682:694	ergothioneine	682:694	ergothioneine (GAER)	682:701	The results indicate that treatment with gum arabic and ergothioneine (GAER) retarded nucleotide breakdown, lipid oxidation, protein degradation, and reduced microbial growth compare with the control.
24799212	7	26	theme	storage	1201:1207	arg1	life					1209:1212	its storage life	1197:1212	its storage life	1197:1212	Our study suggests that GAER treatment has potential to improve the quality of red sea bream and extend its storage life.
24799212	0	27	theme	quality	30:36	arg1	changes					38:44	quality changes	30:44	quality changes	30:44	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	2	28	theme	thiobarbituric	466:479	arg1	TBA					487:489	TBA	487:489	TBA	487:489	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	2	28	theme	thiobarbituric	466:479	arg1	acid					481:484	thiobarbituric acid	466:484	thiobarbituric acid (TBA)	466:490	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	1	29	theme	gum	195:197	arg1	GA					215:216	GA	215:216	GA	215:216	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	1	29	theme	gum	195:197	arg1	coating					206:212	gum arabic coating	195:212	gum arabic coating (GA)	195:217	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	2	30	theme	pH	417:418	arg1	value					420:424	pH value	417:424	pH value	417:424	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	1	31	theme	sensory	259:265	arg1	characteristics					287:301	the sensory and physicochemical characteristics	255:301	the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days	255:368	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	0	32	theme	ergothioneine	118:130	arg1	treatment					132:140	ergothioneine treatment	118:140	ergothioneine treatment	118:140	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	5	33	theme	overall	960:966	arg1	quality					968:974	the overall quality	956:974	the overall quality of red sea bream	956:991	Sensory evaluation proved the efficacy of GAER coating by maintaining the overall quality of red sea bream during the storage period.
24799212	1	34	theme	arabic	199:204	arg1	GA					215:216	GA	215:216	GA	215:216	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	1	34	theme	arabic	199:204	arg1	coating					206:212	gum arabic coating	195:212	gum arabic coating (GA)	195:217	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	3	35	theme	lipid	734:738	arg1	oxidation					740:748	lipid oxidation	734:748	lipid oxidation	734:748	The results indicate that treatment with gum arabic and ergothioneine (GAER) retarded nucleotide breakdown, lipid oxidation, protein degradation, and reduced microbial growth compare with the control.
24799212	1	36	theme	±	351:351	arg1	°C					355:356	4 ± 1 °C	349:356	4 ± 1 °C	349:356	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	3	37	dep	growth	794:799	arg1	compare					801:807	compare	801:807	compare	801:807	The results indicate that treatment with gum arabic and ergothioneine (GAER) retarded nucleotide breakdown, lipid oxidation, protein degradation, and reduced microbial growth compare with the control.
24799212	1	38	theme	coating	206:212	arg1	effects					184:190	The combined effects	171:190	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days	171:368	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	0	39	theme	sea	53:55	arg1	Pagrosomus					64:73	Pagrosomus major	64:79	Pagrosomus major	64:79	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	0	39	theme	sea	53:55	arg1	bream					57:61	red sea bream	49:61	red sea bream (Pagrosomus major)	49:80	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	1	40	theme	physicochemical	271:285	arg1	characteristics					287:301	the sensory and physicochemical characteristics	255:301	the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days	255:368	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	2	41	theme	total	427:431	arg1	nitrogen					448:455	total volatile basic nitrogen	427:455	total volatile basic nitrogen (TVB-N)	427:463	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	2	41	theme	total	427:431	arg1	TVB-N					458:462	TVB-N	458:462	TVB-N	458:462	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	0	42	theme	red	49:51	arg1	Pagrosomus					64:73	Pagrosomus major	64:79	Pagrosomus major	64:79	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	0	42	theme	red	49:51	arg1	bream					57:61	red sea bream	49:61	red sea bream (Pagrosomus major)	49:80	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	3	43	theme	gum	667:669	arg1	arabic					671:676	gum arabic	667:676	gum arabic	667:676	The results indicate that treatment with gum arabic and ergothioneine (GAER) retarded nucleotide breakdown, lipid oxidation, protein degradation, and reduced microbial growth compare with the control.
24799212	7	44	theme	GAER	1117:1120	arg1	treatment					1122:1130	GAER treatment	1117:1130	GAER treatment	1117:1130	Our study suggests that GAER treatment has potential to improve the quality of red sea bream and extend its storage life.
24799212	5	45	theme	GAER	928:931	arg1	coating					933:939	GAER coating	928:939	GAER coating	928:939	Sensory evaluation proved the efficacy of GAER coating by maintaining the overall quality of red sea bream during the storage period.
24799212	2	46	theme	sensory	595:601	arg1	quality					603:609	sensory quality	595:609	sensory quality	595:609	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	7	47	theme	red	1172:1174	arg1	bream					1180:1184	red sea bream	1172:1184	red sea bream	1172:1184	Our study suggests that GAER treatment has potential to improve the quality of red sea bream and extend its storage life.
24799212	3	48	theme	protein	751:757	arg1	degradation					759:769	protein degradation	751:769	protein degradation	751:769	The results indicate that treatment with gum arabic and ergothioneine (GAER) retarded nucleotide breakdown, lipid oxidation, protein degradation, and reduced microbial growth compare with the control.
24799212	4	49	dep	GA	866:867	arg1	treatment					875:883	treatment	875:883	treatment	875:883	The efficiency was better than that of GA or ER treatment.
24799212	5	50	theme	coating	933:939	arg1	efficacy					916:923	the efficacy	912:923	the efficacy of GAER coating	912:939	Sensory evaluation proved the efficacy of GAER coating by maintaining the overall quality of red sea bream during the storage period.
24799212	0	51	theme	refrigerated	149:160	arg1	storage					162:168	refrigerated storage	149:168	refrigerated storage	149:168	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	1	52	dep	coating	206:212	arg1	treatment					242:250	treatment	242:250	treatment	242:250	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	2	53	theme	volatile	433:440	arg1	nitrogen					448:455	total volatile basic nitrogen	427:455	total volatile basic nitrogen (TVB-N)	427:463	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	2	53	theme	volatile	433:440	arg1	TVB-N					458:462	TVB-N	458:462	TVB-N	458:462	Fish proximate composition, pH value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), K-value, TCA-soluble peptides, colour, texture profile analyses (TPA), microbiological properties and sensory quality were measured.
24799212	7	54	theme	bream	1180:1184	arg1	quality					1161:1167	the quality	1157:1167	the quality of red sea bream	1157:1184	Our study suggests that GAER treatment has potential to improve the quality of red sea bream and extend its storage life.
24799212	5	55	theme	Sensory	886:892	arg1	evaluation					894:903	Sensory evaluation	886:903	Sensory evaluation	886:903	Sensory evaluation proved the efficacy of GAER coating by maintaining the overall quality of red sea bream during the storage period.
24799212	1	56	from	effects	184:190	arg1	characteristics					287:301	the sensory and physicochemical characteristics	255:301	the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days	255:368	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	0	57	theme	bream	57:61	arg1	responses					16:24	Physicochemical responses	0:24	Physicochemical responses	0:24	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	0	57	theme	bream	57:61	arg1	changes					38:44	quality changes	30:44	quality changes	30:44	Physicochemical responses and quality changes of red sea bream (Pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage.
24799212	1	58	theme	red	306:308	arg1	bream					314:318	red sea bream	306:318	red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days	306:368	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	1	58	theme	red	306:308	arg1	Pagrosomus					321:330	Pagrosomus major	321:336	Pagrosomus major	321:336	The combined effects of gum arabic coating (GA) and ergothioneine (ER) treatment on the sensory and physicochemical characteristics of red sea bream (Pagrosomus major) stored at 4 ± 1 °C for 16 days were investigated.
24799212	3	59	theme	reduced	776:782	arg1	growth					794:799	reduced microbial growth compare	776:807	reduced microbial growth compare	776:807	The results indicate that treatment with gum arabic and ergothioneine (GAER) retarded nucleotide breakdown, lipid oxidation, protein degradation, and reduced microbial growth compare with the control.
24117143	1	0	theme	hybrid	338:343	arg1	scaffolds					345:353	poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds	284:353	poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds	284:353	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	6	1	theme	ECM	1046:1048	arg1	coatings					1050:1057	the different ECM coatings	1032:1057	the different ECM coatings	1032:1057	TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings.
24117143	2	2	theme	different	361:369	arg1	ratios					371:376	Five different ratios	356:376	Five different ratios of chondrocytes and MSCs	356:401	Five different ratios of chondrocytes and MSCs were cocultured to generate cartilage-like ECM within electrospun fibrous scaffolds for 7, 14, and 21 days.
24117143	6	3	theme	coatings	1050:1057	arg1	differences					1017:1027	the subtle differences	1006:1027	the subtle differences of the different ECM coatings	1006:1057	TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings.
24117143	9	4	theme	a	1537:1537	arg1	scaffold					1547:1554	coat a polymer scaffold	1532:1554	coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs	1532:1637	This study demonstrates that it is possible to utilize cocultures of chondrocytes and MSCs to coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs.
24117143	1	5	theme	poly	284:287	arg1	matrix					325:330	poly(ɛ-caprolactone) (PCL)/extracellular matrix	284:330	poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds	284:353	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	1	5	theme	poly	284:287	arg1	ECM					333:335	ECM	333:335	ECM	333:335	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	7	6	theme	expression	1201:1210	arg1	patterns					1212:1219	similar chondrogenic gene expression patterns	1175:1219	similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone	1175:1281	Without TGF-β3, the cartilaginous matrix generated by 1:1 cocultures of chondrocytes to MSCs for 14 days supported similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone.
24117143	4	7	attach	present	718:724	arg1	scaffolds					733:741	the scaffolds	729:741	the scaffolds	729:741	Devitalization was successful at removing cellular matter from the scaffolds, yet did reduce the amount of matrix present in the scaffolds.
24117143	4	7	attach	present	718:724	arg2	matrix					711:716	matrix	711:716	matrix	711:716	Devitalization was successful at removing cellular matter from the scaffolds, yet did reduce the amount of matrix present in the scaffolds.
24117143	4	7	attach	present	718:724	arg2	amount					701:706	the amount	697:706	the amount of matrix present in the scaffolds	697:741	Devitalization was successful at removing cellular matter from the scaffolds, yet did reduce the amount of matrix present in the scaffolds.
24117143	8	8	theme	collagen	1360:1367	arg1	type					1369:1372	collagen type II	1360:1375	collagen type II	1360:1375	These scaffold formulations had a positive chondrogenic effect on aggrecan, collagen type II, and collagen II/I expression when compared to PCL controls.
24117143	0	9	theme	construct	98:106	arg1	devitalization					108:121	construct devitalization	98:121	construct devitalization	98:121	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	9	theme	construct	98:106	arg1	scaffolds					52:60	Cell-derived polymer/extracellular matrix composite scaffolds	0:60	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.	0:169	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	6	10	theme	TGF-β3	892:897	arg1	culture					912:918	TGF-β3 supplemented culture	892:918	TGF-β3 supplemented culture	892:918	TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings.
24117143	7	11	theme	1:1	1114:1116	arg1	cocultures					1118:1127	1:1 cocultures	1114:1127	1:1 cocultures of chondrocytes to MSCs for 14 days	1114:1163	Without TGF-β3, the cartilaginous matrix generated by 1:1 cocultures of chondrocytes to MSCs for 14 days supported similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone.
24117143	9	12	theme	coat	1532:1535	arg1	scaffold					1547:1554	coat a polymer scaffold	1532:1554	coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs	1532:1637	This study demonstrates that it is possible to utilize cocultures of chondrocytes and MSCs to coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs.
24117143	7	13	theme	chondrogenic	1183:1194	arg1	patterns					1212:1219	similar chondrogenic gene expression patterns	1175:1219	similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone	1175:1281	Without TGF-β3, the cartilaginous matrix generated by 1:1 cocultures of chondrocytes to MSCs for 14 days supported similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone.
24117143	0	14	link	Cell-derived	0:11	arg1	devitalization					108:121	construct devitalization	98:121	construct devitalization	98:121	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	14	link	Cell-derived	0:11	arg1	scaffolds					52:60	Cell-derived polymer/extracellular matrix composite scaffolds	0:60	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.	0:169	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	14	link	Cell-derived	0:11	arg1	determination					127:139	determination	127:139	determination of chondroinductive capacity	127:168	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	1	15	theme	chondrogenic	194:205	arg1	potential					207:215	the chondrogenic potential	190:215	the chondrogenic potential of chondrocyte	190:230	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	7	16	theme	gene	1196:1199	arg1	patterns					1212:1219	similar chondrogenic gene expression patterns	1175:1219	similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone	1175:1281	Without TGF-β3, the cartilaginous matrix generated by 1:1 cocultures of chondrocytes to MSCs for 14 days supported similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone.
24117143	2	17	theme	electrospun	457:467	arg1	scaffolds					477:485	electrospun fibrous scaffolds	457:485	electrospun fibrous scaffolds for 7, 14, and 21 days	457:508	Five different ratios of chondrocytes and MSCs were cocultured to generate cartilage-like ECM within electrospun fibrous scaffolds for 7, 14, and 21 days.
24117143	2	18	theme	MSCs	398:401	arg1	ratios					371:376	Five different ratios	356:376	Five different ratios of chondrocytes and MSCs	356:401	Five different ratios of chondrocytes and MSCs were cocultured to generate cartilage-like ECM within electrospun fibrous scaffolds for 7, 14, and 21 days.
24117143	0	19	theme	polymer/extracellular	13:33	arg1	devitalization					108:121	construct devitalization	98:121	construct devitalization	98:121	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	19	theme	polymer/extracellular	13:33	arg1	scaffolds					52:60	Cell-derived polymer/extracellular matrix composite scaffolds	0:60	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.	0:169	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	19	theme	polymer/extracellular	13:33	arg1	determination					127:139	determination	127:139	determination of chondroinductive capacity	127:168	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	6	20	theme	supplemented	899:910	arg1	culture					912:918	TGF-β3 supplemented culture	892:918	TGF-β3 supplemented culture	892:918	TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings.
24117143	7	21	theme	MSCs	1224:1227	arg1	patterns					1212:1219	similar chondrogenic gene expression patterns	1175:1219	similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone	1175:1281	Without TGF-β3, the cartilaginous matrix generated by 1:1 cocultures of chondrocytes to MSCs for 14 days supported similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone.
24117143	4	22	from	scaffolds	733:741	arg1	present					718:724	present	718:724	present	718:724	Devitalization was successful at removing cellular matter from the scaffolds, yet did reduce the amount of matrix present in the scaffolds.
24117143	3	23	theme	chondrogenic	565:576	arg1	effects					578:584	the chondrogenic effects	561:584	the chondrogenic effects of the ECM alone	561:601	These constructs were then devitalized to isolate the chondrogenic effects of the ECM alone.
24117143	0	24	theme	Cell-derived	0:11	arg1	devitalization					108:121	construct devitalization	98:121	construct devitalization	98:121	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	24	theme	Cell-derived	0:11	arg1	scaffolds					52:60	Cell-derived polymer/extracellular matrix composite scaffolds	0:60	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.	0:169	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	24	theme	Cell-derived	0:11	arg1	determination					127:139	determination	127:139	determination of chondroinductive capacity	127:168	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	7	25	theme	cartilaginous	1080:1092	arg1	matrix					1094:1099	the cartilaginous matrix	1076:1099	the cartilaginous matrix generated by 1:1 cocultures of chondrocytes to MSCs for 14 days	1076:1163	Without TGF-β3, the cartilaginous matrix generated by 1:1 cocultures of chondrocytes to MSCs for 14 days supported similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone.
24117143	9	26	theme	capable	1580:1586	arg1	ECM					1576:1578	cartilage-like ECM	1561:1578	cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs	1561:1637	This study demonstrates that it is possible to utilize cocultures of chondrocytes and MSCs to coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs.
24117143	1	27	theme	chondrocyte	220:230	arg1	potential					207:215	the chondrogenic potential	190:215	the chondrogenic potential of chondrocyte	190:230	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	0	28	theme	composite	42:50	arg1	devitalization					108:121	construct devitalization	98:121	construct devitalization	98:121	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	28	theme	composite	42:50	arg1	scaffolds					52:60	Cell-derived polymer/extracellular matrix composite scaffolds	0:60	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.	0:169	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	28	theme	composite	42:50	arg1	determination					127:139	determination	127:139	determination of chondroinductive capacity	127:168	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	29	theme	chondroinductive	144:159	arg1	capacity					161:168	chondroinductive capacity	144:168	chondroinductive capacity	144:168	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	9	30	theme	MSCs	1634:1637	arg1	differentiation					1615:1629	chondrogenic differentiation	1602:1629	chondrogenic differentiation of MSCs	1602:1637	This study demonstrates that it is possible to utilize cocultures of chondrocytes and MSCs to coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs.
24117143	8	31	theme	II/I	1391:1394	arg1	expression					1396:1405	collagen II/I expression	1382:1405	collagen II/I expression	1382:1405	These scaffold formulations had a positive chondrogenic effect on aggrecan, collagen type II, and collagen II/I expression when compared to PCL controls.
24117143	9	32	theme	chondrogenic	1602:1613	arg1	differentiation					1615:1629	chondrogenic differentiation	1602:1629	chondrogenic differentiation of MSCs	1602:1637	This study demonstrates that it is possible to utilize cocultures of chondrocytes and MSCs to coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs.
24117143	2	33	theme	cartilage-like	431:444	arg1	ECM					446:448	cartilage-like ECM	431:448	cartilage-like ECM	431:448	Five different ratios of chondrocytes and MSCs were cocultured to generate cartilage-like ECM within electrospun fibrous scaffolds for 7, 14, and 21 days.
24117143	0	34	theme	matrix	35:40	arg1	devitalization					108:121	construct devitalization	98:121	construct devitalization	98:121	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	34	theme	matrix	35:40	arg1	scaffolds					52:60	Cell-derived polymer/extracellular matrix composite scaffolds	0:60	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.	0:169	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	34	theme	matrix	35:40	arg1	determination					127:139	determination	127:139	determination of chondroinductive capacity	127:168	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	6	35	theme	scaffold	966:973	arg1	type					975:978	each scaffold type	961:978	each scaffold type	961:978	TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings.
24117143	9	36	theme	chondrocytes	1507:1518	arg1	cocultures					1493:1502	cocultures	1493:1502	cocultures of chondrocytes and MSCs	1493:1527	This study demonstrates that it is possible to utilize cocultures of chondrocytes and MSCs to coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs.
24117143	8	37	theme	collagen	1382:1389	arg1	expression					1396:1405	collagen II/I expression	1382:1405	collagen II/I expression	1382:1405	These scaffold formulations had a positive chondrogenic effect on aggrecan, collagen type II, and collagen II/I expression when compared to PCL controls.
24117143	1	38	theme	mesenchymal	236:246	arg1	MSC					259:261	MSC	259:261	MSC	259:261	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	1	38	theme	mesenchymal	236:246	arg1	cell					253:256	mesenchymal stem cell	236:256	mesenchymal stem cell (MSC) coculture	236:272	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	5	39	theme	PCL/ECM	774:780	arg1	scaffolds					782:790	the PCL/ECM scaffolds	770:790	the PCL/ECM scaffolds	770:790	Following devitalization, the PCL/ECM scaffolds were then cultured with MSCs in serum-free conditions with or without TGF-β3 treatment for 21 days.
24117143	6	40	theme	different	1036:1044	arg1	coatings					1050:1057	the different ECM coatings	1032:1057	the different ECM coatings	1032:1057	TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings.
24117143	3	41	theme	ECM	593:595	arg1	effects					578:584	the chondrogenic effects	561:584	the chondrogenic effects of the ECM alone	561:601	These constructs were then devitalized to isolate the chondrogenic effects of the ECM alone.
24117143	2	42	theme	chondrocytes	381:392	arg1	ratios					371:376	Five different ratios	356:376	Five different ratios of chondrocytes and MSCs	356:401	Five different ratios of chondrocytes and MSCs were cocultured to generate cartilage-like ECM within electrospun fibrous scaffolds for 7, 14, and 21 days.
24117143	1	43	theme	stem	248:251	arg1	MSC					259:261	MSC	259:261	MSC	259:261	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	1	43	theme	stem	248:251	arg1	cell					253:256	mesenchymal stem cell	236:256	mesenchymal stem cell (MSC) coculture	236:272	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	1	44	theme	/extracellular	310:323	arg1	matrix					325:330	poly(ɛ-caprolactone) (PCL)/extracellular matrix	284:330	poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds	284:353	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	1	44	theme	/extracellular	310:323	arg1	ECM					333:335	ECM	333:335	ECM	333:335	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	0	45	theme	capacity	161:168	arg1	devitalization					108:121	construct devitalization	98:121	construct devitalization	98:121	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	45	theme	capacity	161:168	arg1	scaffolds					52:60	Cell-derived polymer/extracellular matrix composite scaffolds	0:60	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.	0:169	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	45	theme	capacity	161:168	arg1	determination					127:139	determination	127:139	determination of chondroinductive capacity	127:168	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	8	46	theme	scaffold	1290:1297	arg1	formulations					1299:1310	These scaffold formulations	1284:1310	These scaffold formulations	1284:1310	These scaffold formulations had a positive chondrogenic effect on aggrecan, collagen type II, and collagen II/I expression when compared to PCL controls.
24117143	1	47	theme	cell	253:256	arg1	coculture					264:272	mesenchymal stem cell (MSC) coculture	236:272	mesenchymal stem cell (MSC) coculture	236:272	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	7	48	theme	chondrocytes	1132:1143	arg1	cocultures					1118:1127	1:1 cocultures	1114:1127	1:1 cocultures of chondrocytes to MSCs for 14 days	1114:1163	Without TGF-β3, the cartilaginous matrix generated by 1:1 cocultures of chondrocytes to MSCs for 14 days supported similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone.
24117143	1	49	theme	matrix	325:330	arg1	scaffolds					345:353	poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds	284:353	poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds	284:353	This work examined the chondrogenic potential of chondrocyte and mesenchymal stem cell (MSC) coculture generated poly(ɛ-caprolactone) (PCL)/extracellular matrix (ECM) hybrid scaffolds.
24117143	8	50	theme	chondrogenic	1327:1338	arg1	effect					1340:1345	a positive chondrogenic effect	1316:1345	a positive chondrogenic effect	1316:1345	These scaffold formulations had a positive chondrogenic effect on aggrecan, collagen type II, and collagen II/I expression when compared to PCL controls.
24117143	0	51	theme	cartilage	66:74	arg1	Part					90:93	Part 2	90:95	Part 2	90:95	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	51	theme	cartilage	66:74	arg1	regeneration					76:87	cartilage regeneration	66:87	cartilage regeneration	66:87	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	6	52	from	induction	930:938	arg1	somewhat					990:997	somewhat	990:997	somewhat	990:997	TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings.
24117143	6	52	from	induction	930:938	arg1	masked					999:1004	masked	999:1004	masked the subtle differences of the different ECM coatings	999:1057	TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings.
24117143	6	52	from	induction	930:938	arg1	type					975:978	each scaffold type	961:978	each scaffold type	961:978	TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings.
24117143	9	53	theme	polymer	1539:1545	arg1	scaffold					1547:1554	coat a polymer scaffold	1532:1554	coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs	1532:1637	This study demonstrates that it is possible to utilize cocultures of chondrocytes and MSCs to coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs.
24117143	0	54	dep	scaffolds	52:60	arg1	devitalization					108:121	construct devitalization	98:121	construct devitalization	98:121	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	54	dep	scaffolds	52:60	arg1	scaffolds					52:60	Cell-derived polymer/extracellular matrix composite scaffolds	0:60	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.	0:169	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	0	54	dep	scaffolds	52:60	arg1	determination					127:139	determination	127:139	determination of chondroinductive capacity	127:168	Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.
24117143	4	55	from	present	718:724	arg1	scaffolds					733:741	the scaffolds	729:741	the scaffolds	729:741	Devitalization was successful at removing cellular matter from the scaffolds, yet did reduce the amount of matrix present in the scaffolds.
24117143	4	56	theme	present	718:724	arg1	matrix					711:716	matrix	711:716	matrix	711:716	Devitalization was successful at removing cellular matter from the scaffolds, yet did reduce the amount of matrix present in the scaffolds.
24117143	4	56	theme	present	718:724	arg1	amount					701:706	the amount	697:706	the amount of matrix present in the scaffolds	697:741	Devitalization was successful at removing cellular matter from the scaffolds, yet did reduce the amount of matrix present in the scaffolds.
24117143	8	57	contain	had	1312:1314	arg1	formulations					1299:1310	These scaffold formulations	1284:1310	These scaffold formulations	1284:1310	These scaffold formulations had a positive chondrogenic effect on aggrecan, collagen type II, and collagen II/I expression when compared to PCL controls.
24117143	8	57	contain	had	1312:1314	arg2	effect					1340:1345	a positive chondrogenic effect	1316:1345	a positive chondrogenic effect	1316:1345	These scaffold formulations had a positive chondrogenic effect on aggrecan, collagen type II, and collagen II/I expression when compared to PCL controls.
24117143	9	58	theme	cartilage-like	1561:1574	arg1	ECM					1576:1578	cartilage-like ECM	1561:1578	cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs	1561:1637	This study demonstrates that it is possible to utilize cocultures of chondrocytes and MSCs to coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs.
24117143	8	59	theme	PCL	1424:1426	arg1	controls					1428:1435	PCL controls	1424:1435	PCL controls	1424:1435	These scaffold formulations had a positive chondrogenic effect on aggrecan, collagen type II, and collagen II/I expression when compared to PCL controls.
24117143	4	60	theme	matrix	711:716	arg1	matrix					711:716	matrix	711:716	matrix	711:716	Devitalization was successful at removing cellular matter from the scaffolds, yet did reduce the amount of matrix present in the scaffolds.
24117143	4	60	theme	matrix	711:716	arg1	amount					701:706	the amount	697:706	the amount of matrix present in the scaffolds	697:741	Devitalization was successful at removing cellular matter from the scaffolds, yet did reduce the amount of matrix present in the scaffolds.
24117143	8	61	theme	positive	1318:1325	arg1	effect					1340:1345	a positive chondrogenic effect	1316:1345	a positive chondrogenic effect	1316:1345	These scaffold formulations had a positive chondrogenic effect on aggrecan, collagen type II, and collagen II/I expression when compared to PCL controls.
24117143	9	62	with	scaffold	1547:1554	arg1	ECM					1576:1578	cartilage-like ECM	1561:1578	cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs	1561:1637	This study demonstrates that it is possible to utilize cocultures of chondrocytes and MSCs to coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs.
24117143	4	63	theme	cellular	646:653	arg1	matter					655:660	cellular matter	646:660	cellular matter	646:660	Devitalization was successful at removing cellular matter from the scaffolds, yet did reduce the amount of matrix present in the scaffolds.
24117143	5	64	theme	serum-free	824:833	arg1	conditions					835:844	serum-free conditions	824:844	serum-free conditions	824:844	Following devitalization, the PCL/ECM scaffolds were then cultured with MSCs in serum-free conditions with or without TGF-β3 treatment for 21 days.
24117143	9	65	theme	MSCs	1524:1527	arg1	cocultures					1493:1502	cocultures	1493:1502	cocultures of chondrocytes and MSCs	1493:1527	This study demonstrates that it is possible to utilize cocultures of chondrocytes and MSCs to coat a polymer scaffold with cartilage-like ECM capable of supporting chondrogenic differentiation of MSCs.
24117143	5	66	theme	TGF-β3	862:867	arg1	treatment					869:877	TGF-β3 treatment	862:877	TGF-β3 treatment for 21 days	862:889	Following devitalization, the PCL/ECM scaffolds were then cultured with MSCs in serum-free conditions with or without TGF-β3 treatment for 21 days.
24117143	6	67	theme	subtle	1010:1015	arg1	differences					1017:1027	the subtle differences	1006:1027	the subtle differences of the different ECM coatings	1006:1057	TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings.
24117143	2	68	theme	fibrous	469:475	arg1	scaffolds					477:485	electrospun fibrous scaffolds	457:485	electrospun fibrous scaffolds for 7, 14, and 21 days	457:508	Five different ratios of chondrocytes and MSCs were cocultured to generate cartilage-like ECM within electrospun fibrous scaffolds for 7, 14, and 21 days.
24117143	7	69	theme	similar	1175:1181	arg1	patterns					1212:1219	similar chondrogenic gene expression patterns	1175:1219	similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone	1175:1281	Without TGF-β3, the cartilaginous matrix generated by 1:1 cocultures of chondrocytes to MSCs for 14 days supported similar chondrogenic gene expression patterns of MSCs cultured on scaffolds generated by chondrocytes alone.
24117143	6	70	theme	chondrogenesis	943:956	arg1	induction					930:938	an induction	927:938	an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings	927:1057	TGF-β3 supplemented culture caused an induction of chondrogenesis in each scaffold type, but also somewhat masked the subtle differences of the different ECM coatings.
28098414	6	0	theme	binding	1015:1021	arg1	capacity					1023:1030	higher binding capacity	1008:1030	higher binding capacity for melamine imprinted membranes	1008:1063	The results showed higher binding capacity for melamine imprinted membranes in comparison with the nonimprinted membranes.
28098414	2	1	theme	template	609:616	arg1	molecule					618:625	the template molecule	605:625	the template molecule	605:625	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	8	2	theme	quantification	1389:1402	arg1	limit					1366:1370	The limit	1362:1370	The limit of detection and quantification of melamine	1362:1414	The limit of detection and quantification of melamine was 0.007 and 0.020 μg/mL, respectively.
28098414	8	2	theme	quantification	1389:1402	arg1	0.007					1420:1424	0.007	1420:1424	0.007	1420:1424	The limit of detection and quantification of melamine was 0.007 and 0.020 μg/mL, respectively.
28098414	3	3	theme	transmission	700:711	arg1	spectroscopy					722:733	Fourier transmission infrared spectroscopy	692:733	Fourier transmission infrared spectroscopy	692:733	The characterization of the polymeric membrane was performed by Fourier transmission infrared spectroscopy and scanning electron microscopy.
28098414	7	4	theme	excellent	1332:1340	arg1	precision					1342:1350	an excellent precision	1329:1350	an excellent precision of 2.73%	1329:1359	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	3	5	theme	infrared	713:720	arg1	spectroscopy					722:733	Fourier transmission infrared spectroscopy	692:733	Fourier transmission infrared spectroscopy	692:733	The characterization of the polymeric membrane was performed by Fourier transmission infrared spectroscopy and scanning electron microscopy.
28098414	5	6	theme	extraction	935:944	arg1	efficiency					946:955	the extraction efficiency	931:955	the extraction efficiency of the membrane	931:971	Various parameters affecting the extraction efficiency of the membrane were evaluated.
28098414	7	7	from	curve	1285:1289	arg1	range					1298:1302	the range	1294:1302	the range of 0.02-11.80 μg/mL	1294:1322	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	6	8	theme	imprinted	1045:1053	arg1	membranes					1055:1063	melamine imprinted membranes	1036:1063	melamine imprinted membranes	1036:1063	The results showed higher binding capacity for melamine imprinted membranes in comparison with the nonimprinted membranes.
28098414	4	9	theme	integrated	774:783	arg1	medium					841:846	a solid-phase extraction medium	816:846	a solid-phase extraction medium	816:846	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	4	9	theme	integrated	774:783	arg1	membrane					795:802	This integrated composite membrane	769:802	This integrated composite membrane	769:802	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	4	10	from	samples	893:899	arg1	extraction					856:865	the extraction	852:865	the extraction of melamine from dry milk samples	852:899	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	7	11	theme	dry	1204:1206	arg1	milk					1208:1211	dry milk	1204:1211	dry milk	1204:1211	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	6	12	theme	melamine	1036:1043	arg1	membranes					1055:1063	melamine imprinted membranes	1036:1063	melamine imprinted membranes	1036:1063	The results showed higher binding capacity for melamine imprinted membranes in comparison with the nonimprinted membranes.
28098414	2	13	theme	ethylene	516:523	arg1	monomer					507:513	a functional monomer	494:513	a functional monomer	494:513	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	2	13	theme	ethylene	516:523	arg1	glycol					525:530	ethylene glycol	516:530	ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule	516:625	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	4	14	theme	dry	884:886	arg1	samples					893:899	dry milk samples	884:899	dry milk samples	884:899	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	6	15	theme	nonimprinted	1088:1099	arg1	membranes					1101:1109	the nonimprinted membranes	1084:1109	the nonimprinted membranes	1084:1109	The results showed higher binding capacity for melamine imprinted membranes in comparison with the nonimprinted membranes.
28098414	9	16	theme	%	1514:1514	arg1	range					1496:1500	the range	1492:1500	the range of 88.7-94.8%	1492:1514	The recoveries of melamine were in the range of 88.7-94.8%.
28098414	0	17	theme	melamine	111:118	arg1	extraction					97:106	the extraction	93:106	the extraction of melamine from dry milk before HPLC analysis	93:153	Integrated photografted molecularly imprinted polymers with a cellulose acetate membrane for the extraction of melamine from dry milk before HPLC analysis.
28098414	3	18	theme	scanning	739:746	arg1	microscopy					757:766	scanning electron microscopy	739:766	scanning electron microscopy	739:766	The characterization of the polymeric membrane was performed by Fourier transmission infrared spectroscopy and scanning electron microscopy.
28098414	7	19	theme	melamine	1190:1197	arg1	extraction					1176:1185	the extraction	1172:1185	the extraction of melamine from dry milk by the photografted cellulose acetate membrane	1172:1258	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	7	20	contain	had	1260:1262	arg1	extraction					1176:1185	the extraction	1172:1185	the extraction of melamine from dry milk by the photografted cellulose acetate membrane	1172:1258	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	7	20	contain	had	1260:1262	arg2	curve					1285:1289	a linear calibration curve	1264:1289	a linear calibration curve in the range of 0.02-11.80 μg/mL	1264:1322	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	7	21	theme	linear	1266:1271	arg1	curve					1285:1289	a linear calibration curve	1264:1289	a linear calibration curve in the range of 0.02-11.80 μg/mL	1264:1322	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	5	22	theme	Various	902:908	arg1	parameters					910:919	Various parameters	902:919	Various parameters affecting the extraction efficiency of the membrane	902:971	Various parameters affecting the extraction efficiency of the membrane were evaluated.
28098414	2	23	theme	polymerization	439:452	arg1	solution					454:461	the polymerization solution	435:461	the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule	435:625	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	3	24	theme	electron	748:755	arg1	microscopy					757:766	scanning electron microscopy	739:766	scanning electron microscopy	739:766	The characterization of the polymeric membrane was performed by Fourier transmission infrared spectroscopy and scanning electron microscopy.
28098414	2	25	contain	containing	463:472	arg2	acid					486:489	methacrylic acid	474:489	methacrylic acid	474:489	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	2	25	contain	containing	463:472	arg2	monomer					507:513	a functional monomer	494:513	a functional monomer	494:513	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	2	25	contain	containing	463:472	arg1	solution					454:461	the polymerization solution	435:461	the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule	435:625	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	2	26	theme	cellulose	308:316	arg1	membrane					326:333	The water-compatible cellulose acetate membrane	287:333	The water-compatible cellulose acetate membrane	287:333	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	7	27	theme	%	1359:1359	arg1	precision					1342:1350	an excellent precision	1329:1350	an excellent precision of 2.73%	1329:1359	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	7	28	theme	chromatography	1136:1149	arg1	analysis					1151:1158	High-performance liquid chromatography analysis	1112:1158	High-performance liquid chromatography analysis	1112:1158	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	1	29	theme	melamine	265:272	arg1	determination					248:260	the determination	244:260	the determination of melamine in dry milk	244:284	In this study, a new separation technique based on membrane extraction is described for the determination of melamine in dry milk.
28098414	4	30	theme	milk	888:891	arg1	samples					893:899	dry milk samples	884:899	dry milk samples	884:899	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	2	31	theme	water-compatible	291:306	arg1	membrane					326:333	The water-compatible cellulose acetate membrane	287:333	The water-compatible cellulose acetate membrane	287:333	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	1	32	theme	new	173:175	arg1	technique					188:196	a new separation technique	171:196	a new separation technique based on membrane extraction	171:225	In this study, a new separation technique based on membrane extraction is described for the determination of melamine in dry milk.
28098414	0	33	theme	imprinted	36:44	arg1	polymers					46:53	photografted molecularly imprinted polymers	11:53	photografted molecularly imprinted polymers	11:53	Integrated photografted molecularly imprinted polymers with a cellulose acetate membrane for the extraction of melamine from dry milk before HPLC analysis.
28098414	7	34	from	extraction	1176:1185	arg1	milk					1208:1211	dry milk	1204:1211	dry milk	1204:1211	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	1	35	from	determination	248:260	arg1	milk					281:284	dry milk	277:284	dry milk	277:284	In this study, a new separation technique based on membrane extraction is described for the determination of melamine in dry milk.
28098414	8	36	theme	melamine	1407:1414	arg1	detection					1375:1383	detection	1375:1383	detection	1375:1383	The limit of detection and quantification of melamine was 0.007 and 0.020 μg/mL, respectively.
28098414	8	36	theme	melamine	1407:1414	arg1	quantification					1389:1402	quantification	1389:1402	quantification	1389:1402	The limit of detection and quantification of melamine was 0.007 and 0.020 μg/mL, respectively.
28098414	1	37	theme	separation	177:186	arg1	technique					188:196	a new separation technique	171:196	a new separation technique based on membrane extraction	171:225	In this study, a new separation technique based on membrane extraction is described for the determination of melamine in dry milk.
28098414	1	38	theme	dry	277:279	arg1	milk					281:284	dry milk	277:284	dry milk	277:284	In this study, a new separation technique based on membrane extraction is described for the determination of melamine in dry milk.
28098414	0	39	theme	dry	125:127	arg1	milk					129:132	dry milk	125:132	dry milk before HPLC analysis	125:153	Integrated photografted molecularly imprinted polymers with a cellulose acetate membrane for the extraction of melamine from dry milk before HPLC analysis.
28098414	3	40	theme	polymeric	656:664	arg1	membrane					666:673	the polymeric membrane	652:673	the polymeric membrane	652:673	The characterization of the polymeric membrane was performed by Fourier transmission infrared spectroscopy and scanning electron microscopy.
28098414	4	41	theme	composite	785:793	arg1	medium					841:846	a solid-phase extraction medium	816:846	a solid-phase extraction medium	816:846	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	4	41	theme	composite	785:793	arg1	membrane					795:802	This integrated composite membrane	769:802	This integrated composite membrane	769:802	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	7	42	theme	acetate	1243:1249	arg1	membrane					1251:1258	the photografted cellulose acetate membrane	1216:1258	the photografted cellulose acetate membrane	1216:1258	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	7	43	theme	cellulose	1233:1241	arg1	membrane					1251:1258	the photografted cellulose acetate membrane	1216:1258	the photografted cellulose acetate membrane	1216:1258	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	6	44	theme	higher	1008:1013	arg1	capacity					1023:1030	higher binding capacity	1008:1030	higher binding capacity for melamine imprinted membranes	1008:1063	The results showed higher binding capacity for melamine imprinted membranes in comparison with the nonimprinted membranes.
28098414	0	45	theme	HPLC	141:144	arg1	analysis					146:153	HPLC analysis	141:153	HPLC analysis	141:153	Integrated photografted molecularly imprinted polymers with a cellulose acetate membrane for the extraction of melamine from dry milk before HPLC analysis.
28098414	3	46	theme	membrane	666:673	arg1	characterization					632:647	The characterization	628:647	The characterization of the polymeric membrane	628:673	The characterization of the polymeric membrane was performed by Fourier transmission infrared spectroscopy and scanning electron microscopy.
28098414	2	47	theme	imprinted	370:378	arg1	nanospheres					380:390	melamine imprinted nanospheres	361:390	melamine imprinted nanospheres	361:390	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	2	48	theme	methacrylic	474:484	arg1	monomer					507:513	a functional monomer	494:513	a functional monomer	494:513	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	2	48	theme	methacrylic	474:484	arg1	acid					486:489	methacrylic acid	474:489	methacrylic acid	474:489	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	2	49	dep	glycol	525:530	arg1	dimethacrylate					532:545	dimethacrylate	532:545	dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule	532:625	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	6	50	with	comparison	1068:1077	arg1	membranes					1101:1109	the nonimprinted membranes	1084:1109	the nonimprinted membranes	1084:1109	The results showed higher binding capacity for melamine imprinted membranes in comparison with the nonimprinted membranes.
28098414	8	51	theme	detection	1375:1383	arg1	limit					1366:1370	The limit	1362:1370	The limit of detection and quantification of melamine	1362:1414	The limit of detection and quantification of melamine was 0.007 and 0.020 μg/mL, respectively.
28098414	8	51	theme	detection	1375:1383	arg1	0.007					1420:1424	0.007	1420:1424	0.007	1420:1424	The limit of detection and quantification of melamine was 0.007 and 0.020 μg/mL, respectively.
28098414	0	52	theme	cellulose	62:70	arg1	membrane					80:87	a cellulose acetate membrane	60:87	a cellulose acetate membrane for the extraction of melamine from dry milk before HPLC analysis	60:153	Integrated photografted molecularly imprinted polymers with a cellulose acetate membrane for the extraction of melamine from dry milk before HPLC analysis.
28098414	7	53	theme	High-performance	1112:1127	arg1	chromatography					1136:1149	High-performance liquid chromatography	1112:1149	High-performance liquid chromatography analysis	1112:1158	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	2	54	theme	functional	496:505	arg1	monomer					507:513	a functional monomer	494:513	a functional monomer	494:513	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	2	54	theme	functional	496:505	arg1	glycol					525:530	ethylene glycol	516:530	ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule	516:625	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	2	54	theme	functional	496:505	arg1	acid					486:489	methacrylic acid	474:489	methacrylic acid	474:489	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	4	55	theme	melamine	870:877	arg1	extraction					856:865	the extraction	852:865	the extraction of melamine from dry milk samples	852:899	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	7	56	theme	photografted	1220:1231	arg1	membrane					1251:1258	the photografted cellulose acetate membrane	1216:1258	the photografted cellulose acetate membrane	1216:1258	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	7	57	from	milk	1208:1211	arg1	melamine					1190:1197	melamine	1190:1197	melamine from dry milk	1190:1211	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	7	57	from	milk	1208:1211	arg1	extraction					1176:1185	the extraction	1172:1185	the extraction of melamine from dry milk by the photografted cellulose acetate membrane	1172:1258	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	7	58	theme	liquid	1129:1134	arg1	chromatography					1136:1149	High-performance liquid chromatography	1112:1149	High-performance liquid chromatography analysis	1112:1158	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	5	59	theme	membrane	964:971	arg1	efficiency					946:955	the extraction efficiency	931:955	the extraction efficiency of the membrane	931:971	Various parameters affecting the extraction efficiency of the membrane were evaluated.
28098414	3	60	theme	Fourier	692:698	arg1	spectroscopy					722:733	Fourier transmission infrared spectroscopy	692:733	Fourier transmission infrared spectroscopy	692:733	The characterization of the polymeric membrane was performed by Fourier transmission infrared spectroscopy and scanning electron microscopy.
28098414	4	61	theme	extraction	830:839	arg1	medium					841:846	a solid-phase extraction medium	816:846	a solid-phase extraction medium	816:846	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	4	61	theme	extraction	830:839	arg1	membrane					795:802	This integrated composite membrane	769:802	This integrated composite membrane	769:802	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	1	62	theme	membrane	207:214	arg1	extraction					216:225	membrane extraction	207:225	membrane extraction	207:225	In this study, a new separation technique based on membrane extraction is described for the determination of melamine in dry milk.
28098414	4	63	theme	solid-phase	818:828	arg1	medium					841:846	a solid-phase extraction medium	816:846	a solid-phase extraction medium	816:846	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	4	63	theme	solid-phase	818:828	arg1	membrane					795:802	This integrated composite membrane	769:802	This integrated composite membrane	769:802	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	2	64	theme	acetate	318:324	arg1	membrane					326:333	The water-compatible cellulose acetate membrane	287:333	The water-compatible cellulose acetate membrane	287:333	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	9	65	theme	melamine	1475:1482	arg1	recoveries					1461:1470	The recoveries	1457:1470	The recoveries of melamine	1457:1482	The recoveries of melamine were in the range of 88.7-94.8%.
28098414	7	66	theme	0.02-11.80 μg/mL	1307:1322	arg1	range					1298:1302	the range	1294:1302	the range of 0.02-11.80 μg/mL	1294:1322	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	2	67	theme	melamine	361:368	arg1	nanospheres					380:390	melamine imprinted nanospheres	361:390	melamine imprinted nanospheres	361:390	The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule.
28098414	4	68	used	used	808:811	arg2	membrane					795:802	This integrated composite membrane	769:802	This integrated composite membrane	769:802	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	4	68	used	used	808:811	arg2	medium					841:846	a solid-phase extraction medium	816:846	a solid-phase extraction medium	816:846	This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples.
28098414	0	69	from	milk	129:132	arg1	extraction					97:106	the extraction	93:106	the extraction of melamine from dry milk before HPLC analysis	93:153	Integrated photografted molecularly imprinted polymers with a cellulose acetate membrane for the extraction of melamine from dry milk before HPLC analysis.
28098414	0	70	theme	acetate	72:78	arg1	membrane					80:87	a cellulose acetate membrane	60:87	a cellulose acetate membrane for the extraction of melamine from dry milk before HPLC analysis	60:153	Integrated photografted molecularly imprinted polymers with a cellulose acetate membrane for the extraction of melamine from dry milk before HPLC analysis.
28098414	7	71	theme	calibration	1273:1283	arg1	curve					1285:1289	a linear calibration curve	1264:1289	a linear calibration curve in the range of 0.02-11.80 μg/mL	1264:1322	High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 μg/mL with an excellent precision of 2.73%.
28098414	0	72	theme	photografted	11:22	arg1	polymers					46:53	photografted molecularly imprinted polymers	11:53	photografted molecularly imprinted polymers	11:53	Integrated photografted molecularly imprinted polymers with a cellulose acetate membrane for the extraction of melamine from dry milk before HPLC analysis.
26287313	7	0	theme	related	1491:1497	arg1	changes					1483:1489	cell wall developmental changes	1459:1489	cell wall developmental changes related to support or defence functions independent of their neighbours	1459:1561	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	4	1	theme	similar	843:849	arg1	type					851:854	a similar type	841:854	a similar type of cell wall	841:867	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	2	2	theme	cell	353:356	arg1	walls					358:362	Tracheid cell walls	344:362	Tracheid cell walls in compression wood	344:382	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	7	3	theme	developmental	1469:1481	arg1	changes					1483:1489	cell wall developmental changes	1459:1489	cell wall developmental changes related to support or defence functions independent of their neighbours	1459:1561	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	6	4	theme	fluorophores	1166:1177	arg1	microscopy					1142:1151	UV/visible fluorescence microscopy	1118:1151	UV/visible fluorescence microscopy of cell wall fluorophores	1118:1177	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	6	4	theme	fluorophores	1166:1177	arg1	immunocytochemistry					1183:1201	immunocytochemistry	1183:1201	immunocytochemistry of galactan and mannan epitopes	1183:1233	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	6	5	theme	parenchyma	1262:1271	arg1	walls					1278:1282	xylem parenchyma cell walls	1256:1282	xylem parenchyma cell walls	1256:1282	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	3	6	theme	parenchyma	549:558	arg1	walls					565:569	Xylem parenchyma cell walls	543:569	Xylem parenchyma cell walls	543:569	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	7	7	theme	cell	1459:1462	arg1	changes					1483:1489	cell wall developmental changes	1459:1489	cell wall developmental changes related to support or defence functions independent of their neighbours	1459:1561	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	6	8	theme	cell	1156:1159	arg1	fluorophores					1166:1177	cell wall fluorophores	1156:1177	cell wall fluorophores	1156:1177	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	5	9	from	species	948:954	arg1	normal					917:922	normal	917:922	normal	917:922	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	7	10	theme	types	1404:1408	arg1	cells					1385:1389	Adjacent cells	1376:1389	Adjacent cells of different types but with similar cell wall structure	1376:1445	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	6	11	theme	UV/visible	1118:1127	arg1	microscopy					1142:1151	UV/visible fluorescence microscopy	1118:1151	UV/visible fluorescence microscopy of cell wall fluorophores	1118:1177	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	5	12	theme	walls	908:912	arg1	comparison					872:881	A comparison	870:881	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls	870:1009	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	7	13	theme	Adjacent	1376:1383	arg1	cells					1385:1389	Adjacent cells	1376:1389	Adjacent cells of different types but with similar cell wall structure	1376:1445	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	4	14	theme	wall	864:867	arg1	type					851:854	a similar type	841:854	a similar type of cell wall	841:867	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	6	15	theme	mannan	1219:1224	arg1	epitopes					1226:1233	galactan and mannan epitopes	1206:1233	galactan and mannan epitopes	1206:1233	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	4	16	with	parenchyma	711:720	arg1	walls					752:756	lignified secondary cell walls	727:756	lignified secondary cell walls	727:756	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	6	17	theme	galactan	1206:1213	arg1	epitopes					1226:1233	galactan and mannan epitopes	1206:1233	galactan and mannan epitopes	1206:1233	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	5	18	theme	unique	1022:1027	arg1	opportunity					1029:1039	a unique opportunity	1020:1039	a unique opportunity to understand the process of reaction wood formation in conifers	1020:1104	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	7	19	theme	cell	1427:1430	arg1	structure					1437:1445	similar cell wall structure	1419:1445	similar cell wall structure	1419:1445	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	5	20	theme	parenchyma	892:901	arg1	walls					908:912	xylem parenchyma cell walls	886:912	xylem parenchyma cell walls	886:912	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	1	21	theme	cell	332:335	arg1	walls					337:341	secondary cell walls	322:341	secondary cell walls	322:341	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	3	22	theme	lignified	609:617	arg1	spruce					636:641	spruce	636:641	spruce	636:641	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	3	22	theme	lignified	609:617	arg1	walls					629:633	lignified secondary walls	609:633	lignified secondary walls (spruce)	609:642	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	1	23	theme	parenchyma	155:164	arg1	walls					171:175	xylem parenchyma cell walls	149:175	xylem parenchyma cell walls	149:175	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	0	24	theme	compression	67:77	arg1	wood					79:82	conifer compression wood	59:82	conifer compression wood	59:82	Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood.
26287313	2	25	theme	woody	529:533	arg1	plants					535:540	woody plants	529:540	woody plants	529:540	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	4	26	theme	cell	747:750	arg1	walls					752:756	lignified secondary cell walls	727:756	lignified secondary cell walls	727:756	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	5	27	from	process	1059:1065	arg1	conifers					1097:1104	conifers	1097:1104	conifers	1097:1104	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	7	28	theme	wall	1432:1435	arg1	structure					1437:1445	similar cell wall structure	1419:1445	similar cell wall structure	1419:1445	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	2	29	theme	response	517:524	arg1	part					493:496	part	493:496	part of the gravitropic response of woody plants	493:540	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	6	30	theme	typical	1337:1343	arg1	changes					1300:1306	the changes	1296:1306	the changes in composition and structure typical of compression wood tracheids	1296:1373	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	5	31	theme	wood	1079:1082	arg1	formation					1084:1092	reaction wood formation	1070:1092	reaction wood formation	1070:1092	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	0	32	theme	Xylem	0:4	arg1	walls					22:26	Xylem parenchyma cell walls	0:26	Xylem parenchyma cell walls	0:26	Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood.
26287313	5	33	from	comparison	872:881	arg1	compression					928:938	compression	928:938	compression	928:938	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	5	33	from	comparison	872:881	arg1	normal					917:922	normal	917:922	normal	917:922	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	1	34	theme	Cell	85:88	arg1	fluorescence					95:106	Cell wall fluorescence and immunocytochemistry	85:130	fluorescence	95:106	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	4	35	theme	lignified	727:735	arg1	walls					752:756	lignified secondary cell walls	727:756	lignified secondary cell walls	727:756	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	0	36	theme	cell	17:20	arg1	walls					22:26	Xylem parenchyma cell walls	0:26	Xylem parenchyma cell walls	0:26	Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood.
26287313	2	37	theme	lean	485:488	arg1	stem					480:483	stem	480:483	stem lean as part of the gravitropic response of woody plants	480:540	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	4	38	theme	comparable	798:807	arg1	features					769:776	features	769:776	features of compression wood comparable to those of tracheids that have a similar type of cell wall	769:867	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	5	39	theme	parenchyma	989:998	arg1	walls					1005:1009	lignified and non-lignified parenchyma cell walls	961:1009	lignified and non-lignified parenchyma cell walls	961:1009	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	2	40	theme	Tracheid	344:351	arg1	walls					358:362	Tracheid cell walls	344:362	Tracheid cell walls in compression wood	344:382	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	0	41	theme	gravitropic	35:45	arg1	response					47:54	a gravitropic response	33:54	a gravitropic response in conifer compression wood	33:82	Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood.
26287313	6	42	from	changes	1300:1306	arg1	composition					1311:1321	composition	1311:1321	composition	1311:1321	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	6	42	from	changes	1300:1306	arg1	structure					1327:1335	structure	1327:1335	structure	1327:1335	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	8	43	theme	gravitropic	1591:1601	arg1	signals					1603:1609	gravitropic signals	1591:1609	gravitropic signals	1591:1609	Tracheids are sensitive to gravitropic signals while xylem parenchyma cells are not.
26287313	5	44	from	normal	917:922	arg1	species					948:954	species	948:954	species with lignified and non-lignified parenchyma cell walls	948:1009	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	1	45	theme	related	226:232	arg1	structure					200:208	structure	200:208	structure	200:208	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	3	46	theme	primary	659:665	arg1	pine					674:677	pine	674:677	pine	674:677	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	3	46	theme	primary	659:665	arg1	walls					667:671	unlignified primary walls	647:671	unlignified primary walls (pine)	647:678	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	4	47	theme	compression	781:791	arg1	wood					793:796	compression wood	781:796	compression wood	781:796	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	2	48	theme	compression	367:377	arg1	wood					379:382	compression wood	367:382	compression wood	367:382	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	1	49	theme	gravitropic	237:247	arg1	response					249:256	gravitropic response	237:256	gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls	237:341	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	8	50	theme	parenchyma	1623:1632	arg1	cells					1634:1638	xylem parenchyma cells	1617:1638	xylem parenchyma cells	1617:1638	Tracheids are sensitive to gravitropic signals while xylem parenchyma cells are not.
26287313	5	51	with	species	948:954	arg1	walls					1005:1009	lignified and non-lignified parenchyma cell walls	961:1009	lignified and non-lignified parenchyma cell walls	961:1009	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	1	52	theme	comparable	258:267	arg1	response					249:256	gravitropic response	237:256	gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls	237:341	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	7	53	theme	wall	1464:1467	arg1	changes					1483:1489	cell wall developmental changes	1459:1489	cell wall developmental changes related to support or defence functions independent of their neighbours	1459:1561	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	6	54	theme	cell	1273:1276	arg1	walls					1278:1282	xylem parenchyma cell walls	1256:1282	xylem parenchyma cell walls	1256:1282	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	3	55	theme	Xylem	543:547	arg1	walls					565:569	Xylem parenchyma cell walls	543:569	Xylem parenchyma cell walls	543:569	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	6	56	theme	wall	1161:1164	arg1	fluorophores					1166:1177	cell wall fluorophores	1156:1177	cell wall fluorophores	1156:1177	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	6	57	theme	xylem	1256:1260	arg1	walls					1278:1282	xylem parenchyma cell walls	1256:1282	xylem parenchyma cell walls	1256:1282	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	2	58	from	walls	358:362	arg1	wood					379:382	compression wood	367:382	compression wood	367:382	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	7	59	theme	similar	1419:1425	arg1	structure					1437:1445	similar cell wall structure	1419:1445	similar cell wall structure	1419:1445	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	6	60	theme	tracheids	1365:1373	arg1	typical					1337:1343	typical	1337:1343	typical	1337:1343	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	6	61	theme	fluorescence	1129:1140	arg1	microscopy					1142:1151	UV/visible fluorescence microscopy	1118:1151	UV/visible fluorescence microscopy of cell wall fluorophores	1118:1177	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	3	62	theme	cell	560:563	arg1	walls					565:569	Xylem parenchyma cell walls	543:569	Xylem parenchyma cell walls	543:569	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	7	63	theme	different	1394:1402	arg1	types					1404:1408	different types	1394:1408	different types	1394:1408	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	2	64	contain	have	384:387	arg1	walls					358:362	Tracheid cell walls	344:362	Tracheid cell walls in compression wood	344:382	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	2	64	contain	have	384:387	arg2	structure					413:421	structure	413:421	structure	413:421	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	2	64	contain	have	384:387	arg2	composition					397:407	altered composition	389:407	altered composition	389:407	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	2	65	theme	plants	535:540	arg1	response					517:524	the gravitropic response	501:524	the gravitropic response of woody plants	501:540	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	6	66	theme	epitopes	1226:1233	arg1	microscopy					1142:1151	UV/visible fluorescence microscopy	1118:1151	UV/visible fluorescence microscopy of cell wall fluorophores	1118:1177	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	6	66	theme	epitopes	1226:1233	arg1	immunocytochemistry					1183:1201	immunocytochemistry	1183:1201	immunocytochemistry of galactan and mannan epitopes	1183:1233	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	3	67	theme	conifer	582:588	arg1	species					590:596	conifer species	582:596	conifer species	582:596	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	4	68	theme	cell	859:862	arg1	wall					864:867	cell wall	859:867	cell wall	859:867	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	3	69	theme	secondary	619:627	arg1	spruce					636:641	spruce	636:641	spruce	636:641	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	3	69	theme	secondary	619:627	arg1	walls					629:633	lignified secondary walls	609:633	lignified secondary walls (spruce)	609:642	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	5	70	theme	xylem	886:890	arg1	walls					908:912	xylem parenchyma cell walls	886:912	xylem parenchyma cell walls	886:912	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	4	71	contain	have	836:839	arg2	type					851:854	a similar type	841:854	a similar type of cell wall	841:867	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	4	71	contain	have	836:839	arg1	tracheids					821:829	tracheids	821:829	tracheids that have a similar type of cell wall	821:867	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	0	72	theme	conifer	59:65	arg1	wood					79:82	conifer compression wood	59:82	conifer compression wood	59:82	Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood.
26287313	1	73	from	changes	189:195	arg1	structure					200:208	structure	200:208	structure	200:208	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	1	73	from	changes	189:195	arg1	composition					214:224	composition	214:224	composition	214:224	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	1	74	theme	xylem	149:153	arg1	walls					171:175	xylem parenchyma cell walls	149:175	xylem parenchyma cell walls	149:175	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	5	75	dep	normal	917:922	arg1	wood					940:943	wood	940:943	wood	940:943	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	6	76	theme	compression	1348:1358	arg1	tracheids					1365:1373	compression wood tracheids	1348:1373	compression wood tracheids	1348:1373	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	1	77	theme	cell	166:169	arg1	walls					171:175	xylem parenchyma cell walls	149:175	xylem parenchyma cell walls	149:175	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	6	78	theme	wood	1360:1363	arg1	tracheids					1365:1373	compression wood tracheids	1348:1373	compression wood tracheids	1348:1373	Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids.
26287313	7	79	theme	neighbours	1552:1561	arg1	independent					1531:1541	independent	1531:1541	independent	1531:1541	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	0	80	from	response	47:54	arg1	wood					79:82	conifer compression wood	59:82	conifer compression wood	59:82	Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood.
26287313	4	81	theme	secondary	737:745	arg1	walls					752:756	lignified secondary cell walls	727:756	lignified secondary cell walls	727:756	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	5	82	theme	reaction	1070:1077	arg1	formation					1084:1092	reaction wood formation	1070:1092	reaction wood formation	1070:1092	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	2	83	theme	gravitropic	505:515	arg1	response					517:524	the gravitropic response	501:524	the gravitropic response of woody plants	501:540	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	2	84	theme	altered	389:395	arg1	composition					397:407	altered composition	389:407	altered composition	389:407	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	0	85	theme	parenchyma	6:15	arg1	walls					22:26	Xylem parenchyma cell walls	0:26	Xylem parenchyma cell walls	0:26	Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood.
26287313	5	86	theme	formation	1084:1092	arg1	process					1059:1065	the process	1055:1065	the process of reaction wood formation in conifers	1055:1104	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	5	87	theme	cell	903:906	arg1	walls					908:912	xylem parenchyma cell walls	886:912	xylem parenchyma cell walls	886:912	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	2	88	theme	responsible	450:460	arg1	strain					443:448	the strain	439:448	the strain responsible for correction of stem lean as part of the gravitropic response of woody plants	439:540	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	4	89	theme	xylem	705:709	arg1	parenchyma					711:720	xylem parenchyma	705:720	xylem parenchyma with lignified secondary cell walls	705:756	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	1	90	theme	wall	90:93	arg1	fluorescence					95:106	Cell wall fluorescence and immunocytochemistry	85:130	fluorescence	95:106	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	5	91	theme	non-lignified	975:987	arg1	walls					1005:1009	lignified and non-lignified parenchyma cell walls	961:1009	lignified and non-lignified parenchyma cell walls	961:1009	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	2	92	theme	stem	480:483	arg1	correction					466:475	correction	466:475	correction of stem lean as part of the gravitropic response of woody plants	466:540	Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants.
26287313	4	93	theme	wood	793:796	arg1	features					769:776	features	769:776	features of compression wood comparable to those of tracheids that have a similar type of cell wall	769:867	It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall.
26287313	7	94	with	cells	1385:1389	arg1	structure					1437:1445	similar cell wall structure	1419:1445	similar cell wall structure	1419:1445	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	7	95	theme	independent	1531:1541	arg1	functions					1521:1529	support or defence functions	1502:1529	functions	1521:1529	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	5	96	theme	cell	1000:1003	arg1	walls					1005:1009	lignified and non-lignified parenchyma cell walls	961:1009	lignified and non-lignified parenchyma cell walls	961:1009	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
26287313	1	97	theme	secondary	322:330	arg1	walls					337:341	secondary cell walls	322:341	secondary cell walls	322:341	Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls.
26287313	7	98	theme	defence	1513:1519	arg1	functions					1521:1529	support or defence functions	1502:1529	functions	1521:1529	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	8	99	theme	xylem	1617:1621	arg1	cells					1634:1638	xylem parenchyma cells	1617:1638	xylem parenchyma cells	1617:1638	Tracheids are sensitive to gravitropic signals while xylem parenchyma cells are not.
26287313	3	100	theme	unlignified	647:657	arg1	pine					674:677	pine	674:677	pine	674:677	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	3	100	theme	unlignified	647:657	arg1	walls					667:671	unlignified primary walls	647:671	unlignified primary walls (pine)	647:678	Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine).
26287313	7	101	theme	support	1502:1508	arg1	functions					1521:1529	support or defence functions	1502:1529	functions	1521:1529	Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours.
26287313	5	102	theme	lignified	961:969	arg1	walls					1005:1009	lignified and non-lignified parenchyma cell walls	961:1009	lignified and non-lignified parenchyma cell walls	961:1009	A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers.
25100321	6	0	theme	biological	948:957	arg1	characteristics					959:973	biological characteristics	948:973	biological characteristics	948:973	The specific functions, composition and biological characteristics of each cuticle layer are discussed with respect to adaptations of Collembola to their soil habitat.
25100321	7	1	theme	cuticle	1161:1167	arg1	chemistry					1177:1185	a rather typical arthropod cuticle surface chemistry	1134:1185	a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography	1134:1244	It was found that the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography.
25100321	3	2	theme	bielanensis	508:518	arg1	cuticle					481:487	the cuticle	477:487	the cuticle of Tetrodontophora bielanensis	477:518	In order to gain a more profound understanding of the cuticle characteristics, the chemical composition and architecture of the cuticle of Tetrodontophora bielanensis was studied.
25100321	4	3	theme	controlled	592:601	arg1	access					603:608	controlled access	592:608	controlled access to each layer that could be analysed separately by chemical spectrometry methods and electron microscopy	592:713	A stepwise removal of the different cuticle layers enabled controlled access to each layer that could be analysed separately by chemical spectrometry methods and electron microscopy.
25100321	7	4	theme	surface	1169:1175	arg1	chemistry					1177:1185	a rather typical arthropod cuticle surface chemistry	1134:1185	a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography	1134:1244	It was found that the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography.
25100321	7	5	theme	typical	1143:1149	arg1	chemistry					1177:1185	a rather typical arthropod cuticle surface chemistry	1134:1185	a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography	1134:1244	It was found that the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography.
25100321	2	6	with	repels	312:317	arg1	efficiency					341:350	remarkable efficiency	330:350	remarkable efficiency	330:350	To avoid suffocating in wet conditions, Collembola have evolved a complex, hierarchically nanostructured, cuticle surface that repels water with remarkable efficiency.
25100321	7	7	theme	arthropod	1151:1159	arg1	chemistry					1177:1185	a rather typical arthropod cuticle surface chemistry	1134:1185	a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography	1134:1244	It was found that the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography.
25100321	3	8	theme	cuticle	481:487	arg1	architecture					461:472	architecture	461:472	architecture	461:472	In order to gain a more profound understanding of the cuticle characteristics, the chemical composition and architecture of the cuticle of Tetrodontophora bielanensis was studied.
25100321	3	8	theme	cuticle	481:487	arg1	composition					445:455	chemical composition	436:455	chemical composition	436:455	In order to gain a more profound understanding of the cuticle characteristics, the chemical composition and architecture of the cuticle of Tetrodontophora bielanensis was studied.
25100321	7	9	theme	cuticle	1227:1233	arg1	topography					1235:1244	the cuticle topography	1223:1244	the cuticle topography	1223:1244	It was found that the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography.
25100321	5	10	theme	base	825:828	arg1	structure					830:838	a chitin-rich lamellar base structure	802:838	a chitin-rich lamellar base structure overlaid by protein-rich nanostructures	802:878	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	5	10	theme	base	825:828	arg1	layers					786:791	three characteristic layers	765:791	three characteristic layers	765:791	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	2	11	dep	complex	251:257	arg1	nanostructured					275:288	nanostructured	275:288	nanostructured	275:288	To avoid suffocating in wet conditions, Collembola have evolved a complex, hierarchically nanostructured, cuticle surface that repels water with remarkable efficiency.
25100321	2	11	dep	complex	251:257	arg1	cuticle					291:297	cuticle	291:297	cuticle	291:297	To avoid suffocating in wet conditions, Collembola have evolved a complex, hierarchically nanostructured, cuticle surface that repels water with remarkable efficiency.
25100321	4	12	theme	spectrometry	670:681	arg1	methods					683:689	chemical spectrometry methods	661:689	chemical spectrometry methods	661:689	A stepwise removal of the different cuticle layers enabled controlled access to each layer that could be analysed separately by chemical spectrometry methods and electron microscopy.
25100321	7	13	theme	topography	1235:1244	arg1	role					1215:1218	the decisive role	1202:1218	the decisive role of the cuticle topography	1202:1244	It was found that the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography.
25100321	6	14	theme	specific	912:919	arg1	functions					921:929	The specific functions	908:929	The specific functions	908:929	The specific functions, composition and biological characteristics of each cuticle layer are discussed with respect to adaptations of Collembola to their soil habitat.
25100321	6	15	theme	Collembola	1042:1051	arg1	adaptations					1027:1037	adaptations	1027:1037	adaptations of Collembola to their soil habitat	1027:1073	The specific functions, composition and biological characteristics of each cuticle layer are discussed with respect to adaptations of Collembola to their soil habitat.
25100321	3	16	theme	chemical	436:443	arg1	composition					445:455	chemical composition	436:455	chemical composition	436:455	In order to gain a more profound understanding of the cuticle characteristics, the chemical composition and architecture of the cuticle of Tetrodontophora bielanensis was studied.
25100321	0	17	theme	multi-layered	4:16	arg1	cuticle					29:35	The multi-layered protective cuticle	0:35	The multi-layered protective cuticle of Collembola	0:49	The multi-layered protective cuticle of Collembola: a chemical analysis.
25100321	4	18	theme	cuticle	569:575	arg1	layers					577:582	the different cuticle layers	555:582	the different cuticle layers	555:582	A stepwise removal of the different cuticle layers enabled controlled access to each layer that could be analysed separately by chemical spectrometry methods and electron microscopy.
25100321	7	19	theme	base	1126:1129	arg1	characteristics					1110:1124	the non-wetting characteristics	1094:1124	the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography	1094:1244	It was found that the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography.
25100321	3	20	theme	characteristics	415:429	arg1	understanding					386:398	a more profound understanding	370:398	a more profound understanding of the cuticle characteristics	370:429	In order to gain a more profound understanding of the cuticle characteristics, the chemical composition and architecture of the cuticle of Tetrodontophora bielanensis was studied.
25100321	3	21	theme	profound	377:384	arg1	understanding					386:398	a more profound understanding	370:398	a more profound understanding of the cuticle characteristics	370:429	In order to gain a more profound understanding of the cuticle characteristics, the chemical composition and architecture of the cuticle of Tetrodontophora bielanensis was studied.
25100321	4	22	theme	different	559:567	arg1	layers					577:582	the different cuticle layers	555:582	the different cuticle layers	555:582	A stepwise removal of the different cuticle layers enabled controlled access to each layer that could be analysed separately by chemical spectrometry methods and electron microscopy.
25100321	5	23	theme	lipid-rich	887:896	arg1	envelope					898:905	a lipid-rich envelope	885:905	a lipid-rich envelope	885:905	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	5	24	theme	overlaid	840:847	arg1	structure					830:838	a chitin-rich lamellar base structure	802:838	a chitin-rich lamellar base structure overlaid by protein-rich nanostructures	802:878	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	5	24	theme	overlaid	840:847	arg1	layers					786:791	three characteristic layers	765:791	three characteristic layers	765:791	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	7	25	theme	non-wetting	1098:1108	arg1	characteristics					1110:1124	the non-wetting characteristics	1094:1124	the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography	1094:1244	It was found that the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography.
25100321	0	26	dep	analysis	63:70	arg1	cuticle					29:35	The multi-layered protective cuticle	0:35	The multi-layered protective cuticle of Collembola	0:49	The multi-layered protective cuticle of Collembola: a chemical analysis.
25100321	5	27	theme	protein-rich	852:863	arg1	nanostructures					865:878	protein-rich nanostructures	852:878	protein-rich nanostructures	852:878	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	0	28	theme	protective	18:27	arg1	cuticle					29:35	The multi-layered protective cuticle	0:35	The multi-layered protective cuticle of Collembola	0:49	The multi-layered protective cuticle of Collembola: a chemical analysis.
25100321	4	29	theme	layers	577:582	arg1	removal					544:550	A stepwise removal	533:550	A stepwise removal of the different cuticle layers	533:582	A stepwise removal of the different cuticle layers enabled controlled access to each layer that could be analysed separately by chemical spectrometry methods and electron microscopy.
25100321	0	30	theme	Collembola	40:49	arg1	cuticle					29:35	The multi-layered protective cuticle	0:35	The multi-layered protective cuticle of Collembola	0:49	The multi-layered protective cuticle of Collembola: a chemical analysis.
25100321	3	31	theme	Tetrodontophora	492:506	arg1	bielanensis					508:518	Tetrodontophora bielanensis	492:518	Tetrodontophora bielanensis	492:518	In order to gain a more profound understanding of the cuticle characteristics, the chemical composition and architecture of the cuticle of Tetrodontophora bielanensis was studied.
25100321	5	32	theme	cuticle	727:733	arg1	composition					735:745	a cuticle composition	725:745	a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope	725:905	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	3	33	theme	cuticle	407:413	arg1	characteristics					415:429	the cuticle characteristics	403:429	the cuticle characteristics	403:429	In order to gain a more profound understanding of the cuticle characteristics, the chemical composition and architecture of the cuticle of Tetrodontophora bielanensis was studied.
25100321	4	34	theme	stepwise	535:542	arg1	removal					544:550	A stepwise removal	533:550	A stepwise removal of the different cuticle layers	533:582	A stepwise removal of the different cuticle layers enabled controlled access to each layer that could be analysed separately by chemical spectrometry methods and electron microscopy.
25100321	7	35	theme	decisive	1206:1213	arg1	role					1215:1218	the decisive role	1202:1218	the decisive role of the cuticle topography	1202:1244	It was found that the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography.
25100321	5	36	theme	chitin-rich	804:814	arg1	structure					830:838	a chitin-rich lamellar base structure	802:838	a chitin-rich lamellar base structure overlaid by protein-rich nanostructures	802:878	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	5	36	theme	chitin-rich	804:814	arg1	layers					786:791	three characteristic layers	765:791	three characteristic layers	765:791	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	6	37	theme	layer	991:995	arg1	functions					921:929	The specific functions	908:929	The specific functions	908:929	The specific functions, composition and biological characteristics of each cuticle layer are discussed with respect to adaptations of Collembola to their soil habitat.
25100321	6	37	theme	layer	991:995	arg1	composition					932:942	composition	932:942	composition	932:942	The specific functions, composition and biological characteristics of each cuticle layer are discussed with respect to adaptations of Collembola to their soil habitat.
25100321	6	37	theme	layer	991:995	arg1	characteristics					959:973	biological characteristics	948:973	biological characteristics	948:973	The specific functions, composition and biological characteristics of each cuticle layer are discussed with respect to adaptations of Collembola to their soil habitat.
25100321	5	38	theme	lamellar	816:823	arg1	structure					830:838	a chitin-rich lamellar base structure	802:838	a chitin-rich lamellar base structure overlaid by protein-rich nanostructures	802:878	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	5	38	theme	lamellar	816:823	arg1	layers					786:791	three characteristic layers	765:791	three characteristic layers	765:791	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	2	39	theme	wet	209:211	arg1	conditions					213:222	wet conditions	209:222	wet conditions	209:222	To avoid suffocating in wet conditions, Collembola have evolved a complex, hierarchically nanostructured, cuticle surface that repels water with remarkable efficiency.
25100321	2	40	theme	remarkable	330:339	arg1	efficiency					341:350	remarkable efficiency	330:350	remarkable efficiency	330:350	To avoid suffocating in wet conditions, Collembola have evolved a complex, hierarchically nanostructured, cuticle surface that repels water with remarkable efficiency.
25100321	3	41	dep	composition	445:455	arg1	the					432:434	the	432:434	the	432:434	In order to gain a more profound understanding of the cuticle characteristics, the chemical composition and architecture of the cuticle of Tetrodontophora bielanensis was studied.
25100321	2	42	theme	complex	251:257	arg1	surface					299:305	a complex, hierarchically nanostructured, cuticle surface	249:305	a complex, hierarchically nanostructured, cuticle surface	249:305	To avoid suffocating in wet conditions, Collembola have evolved a complex, hierarchically nanostructured, cuticle surface that repels water with remarkable efficiency.
25100321	1	43	theme	soil-dwelling	116:128	arg1	Collembola					73:82	Collembola	73:82	Collembola	73:82	Collembola, also known as springtails, are soil-dwelling arthropods that typically respire through the cuticle.
25100321	1	43	theme	soil-dwelling	116:128	arg1	arthropods					130:139	soil-dwelling arthropods	116:139	soil-dwelling arthropods that typically respire through the cuticle	116:182	Collembola, also known as springtails, are soil-dwelling arthropods that typically respire through the cuticle.
25100321	6	44	theme	soil	1062:1065	arg1	habitat					1067:1073	their soil habitat	1056:1073	their soil habitat	1056:1073	The specific functions, composition and biological characteristics of each cuticle layer are discussed with respect to adaptations of Collembola to their soil habitat.
25100321	5	45	theme	characteristic	771:784	arg1	layers					786:791	three characteristic layers	765:791	three characteristic layers	765:791	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	5	45	theme	characteristic	771:784	arg1	structure					830:838	a chitin-rich lamellar base structure	802:838	a chitin-rich lamellar base structure overlaid by protein-rich nanostructures	802:878	We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope.
25100321	4	46	theme	chemical	661:668	arg1	methods					683:689	chemical spectrometry methods	661:689	chemical spectrometry methods	661:689	A stepwise removal of the different cuticle layers enabled controlled access to each layer that could be analysed separately by chemical spectrometry methods and electron microscopy.
25100321	0	47	theme	chemical	54:61	arg1	analysis					63:70	a chemical analysis	52:70	a chemical analysis	52:70	The multi-layered protective cuticle of Collembola: a chemical analysis.
25100321	4	48	theme	electron	695:702	arg1	microscopy					704:713	electron microscopy	695:713	electron microscopy	695:713	A stepwise removal of the different cuticle layers enabled controlled access to each layer that could be analysed separately by chemical spectrometry methods and electron microscopy.
25100321	6	49	theme	cuticle	983:989	arg1	layer					991:995	each cuticle layer	978:995	each cuticle layer	978:995	The specific functions, composition and biological characteristics of each cuticle layer are discussed with respect to adaptations of Collembola to their soil habitat.
27281873	6	0	theme	right	865:869	arg1	condyle					879:885	the right femoral condyle	861:885	the right femoral condyle (control group)	861:901	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	6	0	theme	right	865:869	arg1	group					896:900	control group	888:900	control group	888:900	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	12	1	theme	suitable	2111:2118	arg1	porosity					2120:2127	suitable porosity	2111:2127	suitable porosity	2111:2127	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	10	2	theme	forming	1672:1678	arg1	area					1680:1683	the bone forming area	1663:1683	the bone forming area of the experimental group	1663:1709	At 4, 8, and 12 weeks after implantation, the bone forming area of the experimental group was significantly larger than that of the control group (P < 0.05).
27281873	10	2	theme	forming	1672:1678	arg1	larger					1729:1734	larger	1729:1734	larger	1729:1734	At 4, 8, and 12 weeks after implantation, the bone forming area of the experimental group was significantly larger than that of the control group (P < 0.05).
27281873	2	3	theme	allogeneic	369:378	arg1	powder					385:390	allogeneic bone powder	369:390	allogeneic bone powder	369:390	METHODS The composite porous scaffolds were prepared with chitosan and decalcified allogeneic bone powder at a ratio of 1 : 5 by vacuum freeze-drying technique.
27281873	11	4	theme	positive	1854:1861	arg1	expression					1863:1872	osteoprotegerin (OPG) positive expression	1832:1872	osteoprotegerin (OPG) positive expression	1832:1872	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	12	5	theme	formation	2248:2256	arg1	rate					2258:2261	bone formation rate	2243:2261	bone formation rate	2243:2261	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	7	6	theme	histological	1013:1024	arg1	observation					1026:1036	histological observation	1013:1036	histological observation	1013:1036	At 2, 4, 8, and 12 weeks after implantation, the tissues were harvested for gross observation, histological observation, and immunohistochemical staining.
27281873	8	7	theme	porous	1095:1100	arg1	scaffold					1102:1109	The composite porous scaffold	1081:1109	The composite porous scaffold prepared by vacuum freeze-drying technique	1081:1152	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	11	8	theme	experimental	1891:1902	arg1	group					1904:1908	the experimental group	1887:1908	the experimental group at different time points	1887:1933	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	9	9	dep	observation	1422:1432	arg1	The					1412:1414	The	1412:1414	The	1412:1414	The gross observation and histological observation showed that the defect area was filled with new bone with time, and new bone of the experimental group was significantly more than that of the control group.
27281873	10	10	theme	experimental	1692:1703	arg1	group					1705:1709	the experimental group	1688:1709	the experimental group	1688:1709	At 4, 8, and 12 weeks after implantation, the bone forming area of the experimental group was significantly larger than that of the control group (P < 0.05).
27281873	1	11	theme	powder	179:184	arg1	scaffold					203:210	chitosan/allogeneic bone powder composite porous scaffold	154:210	chitosan/allogeneic bone powder composite porous scaffold	154:210	OBJECTIVE To explore the feasibility of chitosan/allogeneic bone powder composite porous scaffold as scaffold material of bone tissue engineering in repairing bone defect.
27281873	9	12	theme	new	1507:1509	arg1	bone					1511:1514	new bone	1507:1514	new bone with time	1507:1524	The gross observation and histological observation showed that the defect area was filled with new bone with time, and new bone of the experimental group was significantly more than that of the control group.
27281873	11	13	theme	control	2012:2018	arg1	group					2020:2024	the control group	2008:2024	the control group (P < 0.05)	2008:2035	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	11	13	theme	control	2012:2018	arg1	<					2029:2029	P < 0.05	2027:2034	P < 0.05	2027:2034	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	5	14	theme	mm	622:623	arg1	hole					610:613	The hole	606:613	The hole of 3.5 mm in diameter	606:635	The hole of 3.5 mm in diameter was made on the bilateral femoral condyles of 40 adult Sprague Dawley rats.
27281873	6	15	theme	chitosan	748:755	arg1	scaffolds					757:765	chitosan scaffolds	748:765	chitosan scaffolds	748:765	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	11	16	theme	immunohistochemical	1783:1801	arg1	results					1812:1818	The immunohistochemical staining results	1779:1818	The immunohistochemical staining results	1779:1818	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	1	17	theme	porous	196:201	arg1	scaffold					203:210	chitosan/allogeneic bone powder composite porous scaffold	154:210	chitosan/allogeneic bone powder composite porous scaffold	154:210	OBJECTIVE To explore the feasibility of chitosan/allogeneic bone powder composite porous scaffold as scaffold material of bone tissue engineering in repairing bone defect.
27281873	8	18	theme	vacuum	1123:1128	arg1	technique					1144:1152	vacuum freeze-drying technique	1123:1152	vacuum freeze-drying technique	1123:1152	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	12	19	theme	bone	2198:2201	arg1	defect					2203:2208	bone defect	2198:2208	bone defect	2198:2208	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	2	20	theme	porous	308:313	arg1	scaffolds					315:323	The composite porous scaffolds	294:323	The composite porous scaffolds	294:323	METHODS The composite porous scaffolds were prepared with chitosan and decalcified allogeneic bone powder at a ratio of 1 : 5 by vacuum freeze-drying technique.
27281873	11	21	theme	staining	1803:1810	arg1	results					1812:1818	The immunohistochemical staining results	1779:1818	The immunohistochemical staining results	1779:1818	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	12	22	theme	powder	2074:2079	arg1	scaffold					2098:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	5	23	theme	adult	686:690	arg1	rats					707:710	40 adult Sprague Dawley rats	683:710	40 adult Sprague Dawley rats	683:710	The hole of 3.5 mm in diameter was made on the bilateral femoral condyles of 40 adult Sprague Dawley rats.
27281873	8	24	theme	yellowish	1158:1166	arg1	color					1168:1172	yellowish color	1158:1172	yellowish color	1158:1172	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	6	25	theme	control	888:894	arg1	condyle					879:885	the right femoral condyle	861:885	the right femoral condyle (control group)	861:901	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	6	25	theme	control	888:894	arg1	group					896:900	control group	888:900	control group	888:900	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	9	26	theme	new	1531:1533	arg1	bone					1535:1538	new bone	1531:1538	new bone of the experimental group	1531:1564	The gross observation and histological observation showed that the defect area was filled with new bone with time, and new bone of the experimental group was significantly more than that of the control group.
27281873	8	27	theme	=	1392:1392	arg1	scaffold					1365:1372	pure chitosan scaffold	1351:1372	pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09)	1351:1409	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	27	theme	=	1392:1392	arg1	-2.10					1394:1398	t = -2.10	1390:1398	t = -2.10	1390:1398	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	28	theme	pore	1214:1217	arg1	size					1219:1222	pore size	1214:1222	pore size	1214:1222	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	6	29	theme	condyle	879:885	arg1	hole					853:856	the hole	849:856	the hole of the right femoral condyle (control group)	849:901	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	6	29	theme	condyle	879:885	arg1	condyle					816:822	the left femoral condyle	799:822	the left femoral condyle (experimental group)	799:843	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	6	29	theme	condyle	879:885	arg1	group					838:842	experimental group	825:842	experimental group	825:842	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	8	30	theme	pure	1351:1354	arg1	scaffold					1365:1372	pure chitosan scaffold	1351:1372	pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09)	1351:1409	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	30	theme	pure	1351:1354	arg1	-2.10					1394:1398	t = -2.10	1390:1398	t = -2.10	1390:1398	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	30	theme	pure	1351:1354	arg1	%					1386:1386	78.4% ± 1.4%	1375:1386	78.4% ± 1.4%	1375:1386	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	5	31	theme	femoral	663:669	arg1	condyles					671:678	the bilateral femoral condyles	649:678	the bilateral femoral condyles of 40 adult Sprague Dawley rats	649:710	The hole of 3.5 mm in diameter was made on the bilateral femoral condyles of 40 adult Sprague Dawley rats.
27281873	8	32	dep	-2.10	1394:1398	arg1	P					1401:1401	P = 0.09	1401:1408	P = 0.09	1401:1408	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	9	33	theme	experimental	1547:1558	arg1	group					1560:1564	the experimental group	1543:1564	the experimental group	1543:1564	The gross observation and histological observation showed that the defect area was filled with new bone with time, and new bone of the experimental group was significantly more than that of the control group.
27281873	8	34	theme	scaffold	1365:1372	arg1	porosity					1339:1346	the porosity	1335:1346	the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09)	1335:1409	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	2	35	dep	METHODS	286:292	arg1	decalcified					357:367	decalcified	357:367	decalcified allogeneic bone powder at a ratio of 1 : 5 by vacuum freeze-drying technique	357:444	METHODS The composite porous scaffolds were prepared with chitosan and decalcified allogeneic bone powder at a ratio of 1 : 5 by vacuum freeze-drying technique.
27281873	2	35	dep	METHODS	286:292	arg1	prepared					330:337	prepared	330:337	prepared with chitosan	330:351	METHODS The composite porous scaffolds were prepared with chitosan and decalcified allogeneic bone powder at a ratio of 1 : 5 by vacuum freeze-drying technique.
27281873	10	36	theme	P	1768:1768	arg1	<					1770:1770	P < 0.05	1768:1775	P < 0.05	1768:1775	At 4, 8, and 12 weeks after implantation, the bone forming area of the experimental group was significantly larger than that of the control group (P < 0.05).
27281873	10	36	theme	P	1768:1768	arg1	group					1761:1765	the control group	1749:1765	the control group (P < 0.05)	1749:1776	At 4, 8, and 12 weeks after implantation, the bone forming area of the experimental group was significantly larger than that of the control group (P < 0.05).
27281873	12	37	theme	porous	2091:2096	arg1	scaffold					2098:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	11	38	theme	different	1913:1921	arg1	points					1928:1933	different time points	1913:1933	different time points	1913:1933	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	11	39	theme	expression	1953:1962	arg1	level					1964:1968	the positive expression level	1940:1968	the positive expression level	1940:1968	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	11	39	theme	expression	1953:1962	arg1	higher					1988:1993	higher	1988:1993	higher	1988:1993	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	0	40	theme	REPAIR	84:89	arg1	DEFECTS					96:102	REPAIR BONE DEFECTS	84:102	REPAIR BONE DEFECTS IN RATS	84:110	[EXPERIMENTAL STUDY ON CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD TO REPAIR BONE DEFECTS IN RATS].
27281873	1	41	theme	chitosan/allogeneic	154:172	arg1	scaffold					203:210	chitosan/allogeneic bone powder composite porous scaffold	154:210	chitosan/allogeneic bone powder composite porous scaffold	154:210	OBJECTIVE To explore the feasibility of chitosan/allogeneic bone powder composite porous scaffold as scaffold material of bone tissue engineering in repairing bone defect.
27281873	8	42	theme	±	1381:1381	arg1	scaffold					1365:1372	pure chitosan scaffold	1351:1372	pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09)	1351:1409	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	42	theme	±	1381:1381	arg1	%					1386:1386	78.4% ± 1.4%	1375:1386	78.4% ± 1.4%	1375:1386	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	5	43	theme	Dawley	700:705	arg1	rats					707:710	40 adult Sprague Dawley rats	683:710	40 adult Sprague Dawley rats	683:710	The hole of 3.5 mm in diameter was made on the bilateral femoral condyles of 40 adult Sprague Dawley rats.
27281873	11	44	theme	time	1923:1926	arg1	points					1928:1933	different time points	1913:1933	different time points	1913:1933	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	4	45	theme	alternative	492:502	arg1	method					504:509	Ethanol alternative method	484:509	Ethanol alternative method	484:509	Ethanol alternative method was used to measure its porosity, and scanning electron microscopy (SEM) to measure pore size.
27281873	8	46	theme	%	1272:1272	arg1	porosity					1255:1262	the porosity	1251:1262	the porosity	1251:1262	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	46	theme	%	1272:1272	arg1	%					1279:1279	76.8% ± 1.1%	1268:1279	76.8% ± 1.1%	1268:1279	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	0	47	theme	[EXPERIMENTAL	0:12	arg1	STUDY					14:18	[EXPERIMENTAL STUDY	0:18	[EXPERIMENTAL STUDY ON CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD TO REPAIR BONE DEFECTS IN RATS	0:110	[EXPERIMENTAL STUDY ON CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD TO REPAIR BONE DEFECTS IN RATS].
27281873	0	48	from	DEFECTS	96:102	arg1	RATS					107:110	RATS	107:110	RATS	107:110	[EXPERIMENTAL STUDY ON CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD TO REPAIR BONE DEFECTS IN RATS].
27281873	4	49	theme	scanning	549:556	arg1	SEM					579:581	SEM	579:581	SEM	579:581	Ethanol alternative method was used to measure its porosity, and scanning electron microscopy (SEM) to measure pore size.
27281873	4	49	theme	scanning	549:556	arg1	microscopy					567:576	scanning electron microscopy	549:576	scanning electron microscopy (SEM)	549:582	Ethanol alternative method was used to measure its porosity, and scanning electron microscopy (SEM) to measure pore size.
27281873	12	50	theme	chitosan	2293:2300	arg1	scaffold					2302:2309	pure chitosan scaffold	2288:2309	pure chitosan scaffold	2288:2309	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	2	51	theme	vacuum	415:420	arg1	technique					436:444	vacuum freeze-drying technique	415:444	vacuum freeze-drying technique	415:444	METHODS The composite porous scaffolds were prepared with chitosan and decalcified allogeneic bone powder at a ratio of 1 : 5 by vacuum freeze-drying technique.
27281873	4	52	used	used	515:518	arg2	method					504:509	Ethanol alternative method	484:509	Ethanol alternative method	484:509	Ethanol alternative method was used to measure its porosity, and scanning electron microscopy (SEM) to measure pore size.
27281873	5	53	from	hole	610:613	arg1	diameter					628:635	diameter	628:635	diameter	628:635	The hole of 3.5 mm in diameter was made on the bilateral femoral condyles of 40 adult Sprague Dawley rats.
27281873	2	54	theme	5	410:410	arg1	ratio					397:401	a ratio	395:401	a ratio of 1 : 5	395:410	METHODS The composite porous scaffolds were prepared with chitosan and decalcified allogeneic bone powder at a ratio of 1 : 5 by vacuum freeze-drying technique.
27281873	0	55	theme	BONE	43:46	arg1	SCAFFOLD					72:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	[EXPERIMENTAL STUDY ON CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD TO REPAIR BONE DEFECTS IN RATS].
27281873	9	56	theme	gross	1416:1420	arg1	observation					1422:1432	gross observation	1416:1432	gross observation	1416:1432	The gross observation and histological observation showed that the defect area was filled with new bone with time, and new bone of the experimental group was significantly more than that of the control group.
27281873	8	57	theme	=	1403:1403	arg1	P					1401:1401	P = 0.09	1401:1408	P = 0.09	1401:1408	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	6	58	theme	porous	727:732	arg1	scaffolds					734:742	The composite porous scaffolds	713:742	The composite porous scaffolds	713:742	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	7	59	theme	immunohistochemical	1043:1061	arg1	staining					1063:1070	immunohistochemical staining	1043:1070	immunohistochemical staining	1043:1070	At 2, 4, 8, and 12 weeks after implantation, the tissues were harvested for gross observation, histological observation, and immunohistochemical staining.
27281873	6	60	theme	femoral	808:814	arg1	condyle					816:822	the left femoral condyle	799:822	the left femoral condyle (experimental group)	799:843	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	6	60	theme	femoral	808:814	arg1	group					838:842	experimental group	825:842	experimental group	825:842	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	12	61	theme	Chitosan/allogeneic	2049:2067	arg1	scaffold					2098:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	12	62	theme	good	2170:2173	arg1	material					2175:2182	a good material	2168:2182	a good material for repairing bone defect	2168:2208	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	12	62	theme	good	2170:2173	arg1	it					2162:2163	it	2162:2163	it	2162:2163	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	4	63	theme	pore	595:598	arg1	size					600:603	pore size	595:603	pore size	595:603	Ethanol alternative method was used to measure its porosity, and scanning electron microscopy (SEM) to measure pore size.
27281873	1	64	theme	tissue	241:246	arg1	engineering					248:258	bone tissue engineering	236:258	bone tissue engineering	236:258	OBJECTIVE To explore the feasibility of chitosan/allogeneic bone powder composite porous scaffold as scaffold material of bone tissue engineering in repairing bone defect.
27281873	6	65	theme	femoral	871:877	arg1	condyle					879:885	the right femoral condyle	861:885	the right femoral condyle (control group)	861:901	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	6	65	theme	femoral	871:877	arg1	group					896:900	control group	888:900	control group	888:900	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	12	66	theme	bone	2243:2246	arg1	rate					2258:2261	bone formation rate	2243:2261	bone formation rate	2243:2261	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	2	67	theme	bone	380:383	arg1	powder					385:390	allogeneic bone powder	369:390	allogeneic bone powder	369:390	METHODS The composite porous scaffolds were prepared with chitosan and decalcified allogeneic bone powder at a ratio of 1 : 5 by vacuum freeze-drying technique.
27281873	9	68	theme	defect	1479:1484	arg1	area					1486:1489	the defect area	1475:1489	the defect area	1475:1489	The gross observation and histological observation showed that the defect area was filled with new bone with time, and new bone of the experimental group was significantly more than that of the control group.
27281873	10	69	theme	bone	1667:1670	arg1	area					1680:1683	the bone forming area	1663:1683	the bone forming area of the experimental group	1663:1709	At 4, 8, and 12 weeks after implantation, the bone forming area of the experimental group was significantly larger than that of the control group (P < 0.05).
27281873	10	69	theme	bone	1667:1670	arg1	larger					1729:1734	larger	1729:1734	larger	1729:1734	At 4, 8, and 12 weeks after implantation, the bone forming area of the experimental group was significantly larger than that of the control group (P < 0.05).
27281873	2	70	theme	composite	298:306	arg1	scaffolds					315:323	The composite porous scaffolds	294:323	The composite porous scaffolds	294:323	METHODS The composite porous scaffolds were prepared with chitosan and decalcified allogeneic bone powder at a ratio of 1 : 5 by vacuum freeze-drying technique.
27281873	1	71	theme	bone	273:276	arg1	defect					278:283	bone defect	273:283	bone defect	273:283	OBJECTIVE To explore the feasibility of chitosan/allogeneic bone powder composite porous scaffold as scaffold material of bone tissue engineering in repairing bone defect.
27281873	6	72	theme	hole	853:856	arg1	hole					791:794	the hole	787:794	the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group)	787:901	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	12	73	theme	bone	2219:2222	arg1	volume					2232:2237	bone forming volume	2219:2237	bone forming volume	2219:2237	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	12	74	theme	forming	2224:2230	arg1	volume					2232:2237	bone forming volume	2219:2237	bone forming volume	2219:2237	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	10	75	theme	group	1705:1709	arg1	area					1680:1683	the bone forming area	1663:1683	the bone forming area of the experimental group	1663:1709	At 4, 8, and 12 weeks after implantation, the bone forming area of the experimental group was significantly larger than that of the control group (P < 0.05).
27281873	10	75	theme	group	1705:1709	arg1	larger					1729:1734	larger	1729:1734	larger	1729:1734	At 4, 8, and 12 weeks after implantation, the bone forming area of the experimental group was significantly larger than that of the control group (P < 0.05).
27281873	7	76	theme	gross	994:998	arg1	observation					1000:1010	gross observation	994:1010	gross observation	994:1010	At 2, 4, 8, and 12 weeks after implantation, the tissues were harvested for gross observation, histological observation, and immunohistochemical staining.
27281873	1	77	theme	bone	174:177	arg1	scaffold					203:210	chitosan/allogeneic bone powder composite porous scaffold	154:210	chitosan/allogeneic bone powder composite porous scaffold	154:210	OBJECTIVE To explore the feasibility of chitosan/allogeneic bone powder composite porous scaffold as scaffold material of bone tissue engineering in repairing bone defect.
27281873	8	78	theme	composite	1085:1093	arg1	scaffold					1102:1109	The composite porous scaffold	1081:1109	The composite porous scaffold prepared by vacuum freeze-drying technique	1081:1152	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	11	79	from	points	1928:1933	arg1	group					1904:1908	the experimental group	1887:1908	the experimental group at different time points	1887:1933	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	1	80	theme	composite	186:194	arg1	scaffold					203:210	chitosan/allogeneic bone powder composite porous scaffold	154:210	chitosan/allogeneic bone powder composite porous scaffold	154:210	OBJECTIVE To explore the feasibility of chitosan/allogeneic bone powder composite porous scaffold as scaffold material of bone tissue engineering in repairing bone defect.
27281873	0	81	theme	COMPOSITE	55:63	arg1	SCAFFOLD					72:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	[EXPERIMENTAL STUDY ON CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD TO REPAIR BONE DEFECTS IN RATS].
27281873	1	82	theme	scaffold	203:210	arg1	feasibility					139:149	the feasibility	135:149	the feasibility of chitosan/allogeneic bone powder composite porous scaffold	135:210	OBJECTIVE To explore the feasibility of chitosan/allogeneic bone powder composite porous scaffold as scaffold material of bone tissue engineering in repairing bone defect.
27281873	12	83	theme	composite	2081:2089	arg1	scaffold					2098:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	11	84	theme	P	2027:2027	arg1	group					2020:2024	the control group	2008:2024	the control group (P < 0.05)	2008:2035	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	11	84	theme	P	2027:2027	arg1	<					2029:2029	P < 0.05	2027:2034	P < 0.05	2027:2034	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	8	85	contain	had	1154:1156	arg2	color					1168:1172	yellowish color	1158:1172	yellowish color	1158:1172	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	85	contain	had	1154:1156	arg1	scaffold					1102:1109	The composite porous scaffold	1081:1109	The composite porous scaffold prepared by vacuum freeze-drying technique	1081:1152	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	85	contain	had	1154:1156	arg2	brittle					1179:1185	brittle	1179:1185	brittle	1179:1185	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	1	86	theme	scaffold	215:222	arg1	material					224:231	scaffold material	215:231	scaffold material of bone tissue engineering	215:258	OBJECTIVE To explore the feasibility of chitosan/allogeneic bone powder composite porous scaffold as scaffold material of bone tissue engineering in repairing bone defect.
27281873	8	87	theme	freeze-drying	1130:1142	arg1	technique					1144:1152	vacuum freeze-drying technique	1123:1152	vacuum freeze-drying technique	1123:1152	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	88	theme	broken	1198:1203	arg1	texture					1205:1211	easily broken texture	1191:1211	easily broken texture	1191:1211	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	10	89	theme	control	1753:1759	arg1	<					1770:1770	P < 0.05	1768:1775	P < 0.05	1768:1775	At 4, 8, and 12 weeks after implantation, the bone forming area of the experimental group was significantly larger than that of the control group (P < 0.05).
27281873	10	89	theme	control	1753:1759	arg1	group					1761:1765	the control group	1749:1765	the control group (P < 0.05)	1749:1776	At 4, 8, and 12 weeks after implantation, the bone forming area of the experimental group was significantly larger than that of the control group (P < 0.05).
27281873	8	90	theme	chitosan	1356:1363	arg1	scaffold					1365:1372	pure chitosan scaffold	1351:1372	pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09)	1351:1409	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	90	theme	chitosan	1356:1363	arg1	-2.10					1394:1398	t = -2.10	1390:1398	t = -2.10	1390:1398	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	90	theme	chitosan	1356:1363	arg1	%					1386:1386	78.4% ± 1.4%	1375:1386	78.4% ± 1.4%	1375:1386	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	5	91	theme	bilateral	653:661	arg1	condyles					671:678	the bilateral femoral condyles	649:678	the bilateral femoral condyles of 40 adult Sprague Dawley rats	649:710	The hole of 3.5 mm in diameter was made on the bilateral femoral condyles of 40 adult Sprague Dawley rats.
27281873	12	92	theme	bone	2069:2072	arg1	scaffold					2098:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	11	93	located	found	1878:1882	arg2	expression					1863:1872	osteoprotegerin (OPG) positive expression	1832:1872	osteoprotegerin (OPG) positive expression	1832:1872	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	11	93	located	found	1878:1882	arg1	group					1904:1908	the experimental group	1887:1908	the experimental group at different time points	1887:1933	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	9	94	theme	group	1560:1564	arg1	bone					1535:1538	new bone	1531:1538	new bone of the experimental group	1531:1564	The gross observation and histological observation showed that the defect area was filled with new bone with time, and new bone of the experimental group was significantly more than that of the control group.
27281873	8	95	theme	%	1379:1379	arg1	scaffold					1365:1372	pure chitosan scaffold	1351:1372	pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09)	1351:1409	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	95	theme	%	1379:1379	arg1	%					1386:1386	78.4% ± 1.4%	1375:1386	78.4% ± 1.4%	1375:1386	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	0	96	theme	BONE	91:94	arg1	DEFECTS					96:102	REPAIR BONE DEFECTS	84:102	REPAIR BONE DEFECTS IN RATS	84:110	[EXPERIMENTAL STUDY ON CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD TO REPAIR BONE DEFECTS IN RATS].
27281873	0	97	from	STUDY	14:18	arg1	SCAFFOLD					72:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	[EXPERIMENTAL STUDY ON CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD TO REPAIR BONE DEFECTS IN RATS].
27281873	4	98	theme	Ethanol	484:490	arg1	method					504:509	Ethanol alternative method	484:509	Ethanol alternative method	484:509	Ethanol alternative method was used to measure its porosity, and scanning electron microscopy (SEM) to measure pore size.
27281873	8	99	dep	RESULTS	1073:1079	arg1	porosity					1255:1262	the porosity	1251:1262	the porosity	1251:1262	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	99	dep	RESULTS	1073:1079	arg1	μm					1243:1244	200-300 μm	1235:1244	200-300 μm	1235:1244	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	99	dep	RESULTS	1073:1079	arg1	%					1279:1279	76.8% ± 1.1%	1268:1279	76.8% ± 1.1%	1268:1279	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	99	dep	RESULTS	1073:1079	arg1	had					1154:1156	had	1154:1156	had yellowish color, and brittle and easily broken texture	1154:1211	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	12	100	contain	has	2107:2109	arg2	porosity					2120:2127	suitable porosity	2111:2127	suitable porosity	2111:2127	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	12	100	contain	has	2107:2109	arg2	activity					2149:2156	good osteogenic activity	2133:2156	good osteogenic activity	2133:2156	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	12	100	contain	has	2107:2109	arg1	scaffold					2098:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	5	101	theme	rats	707:710	arg1	condyles					671:678	the bilateral femoral condyles	649:678	the bilateral femoral condyles of 40 adult Sprague Dawley rats	649:710	The hole of 3.5 mm in diameter was made on the bilateral femoral condyles of 40 adult Sprague Dawley rats.
27281873	8	102	theme	t	1390:1390	arg1	scaffold					1365:1372	pure chitosan scaffold	1351:1372	pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09)	1351:1409	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	102	theme	t	1390:1390	arg1	-2.10					1394:1398	t = -2.10	1390:1398	t = -2.10	1390:1398	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	12	103	theme	pure	2288:2291	arg1	scaffold					2302:2309	pure chitosan scaffold	2288:2309	pure chitosan scaffold	2288:2309	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	12	104	theme	good	2133:2136	arg1	activity					2149:2156	good osteogenic activity	2133:2156	good osteogenic activity	2133:2156	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	3	105	theme	Chitosan	447:454	arg1	scaffold					456:463	Chitosan scaffold	447:463	Chitosan scaffold	447:463	Chitosan scaffold served as control.
27281873	9	106	theme	control	1606:1612	arg1	group					1614:1618	the control group	1602:1618	the control group	1602:1618	The gross observation and histological observation showed that the defect area was filled with new bone with time, and new bone of the experimental group was significantly more than that of the control group.
27281873	4	107	theme	electron	558:565	arg1	SEM					579:581	SEM	579:581	SEM	579:581	Ethanol alternative method was used to measure its porosity, and scanning electron microscopy (SEM) to measure pore size.
27281873	4	107	theme	electron	558:565	arg1	microscopy					567:576	scanning electron microscopy	549:576	scanning electron microscopy (SEM)	549:582	Ethanol alternative method was used to measure its porosity, and scanning electron microscopy (SEM) to measure pore size.
27281873	0	108	theme	CHITOSAN/ALLOGENEIC	23:41	arg1	SCAFFOLD					72:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	[EXPERIMENTAL STUDY ON CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD TO REPAIR BONE DEFECTS IN RATS].
27281873	2	109	theme	freeze-drying	422:434	arg1	technique					436:444	vacuum freeze-drying technique	415:444	vacuum freeze-drying technique	415:444	METHODS The composite porous scaffolds were prepared with chitosan and decalcified allogeneic bone powder at a ratio of 1 : 5 by vacuum freeze-drying technique.
27281873	8	110	theme	±	1274:1274	arg1	porosity					1255:1262	the porosity	1251:1262	the porosity	1251:1262	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	8	110	theme	±	1274:1274	arg1	%					1279:1279	76.8% ± 1.1%	1268:1279	76.8% ± 1.1%	1268:1279	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	0	111	theme	POWDER	48:53	arg1	SCAFFOLD					72:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	[EXPERIMENTAL STUDY ON CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD TO REPAIR BONE DEFECTS IN RATS].
27281873	6	112	theme	experimental	825:836	arg1	condyle					816:822	the left femoral condyle	799:822	the left femoral condyle (experimental group)	799:843	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	6	112	theme	experimental	825:836	arg1	group					838:842	experimental group	825:842	experimental group	825:842	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	11	113	theme	positive	1944:1951	arg1	level					1964:1968	the positive expression level	1940:1968	the positive expression level	1940:1968	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	11	113	theme	positive	1944:1951	arg1	higher					1988:1993	higher	1988:1993	higher	1988:1993	The immunohistochemical staining results showed that osteoprotegerin (OPG) positive expression was found in the experimental group at different time points, and the positive expression level was significantly higher than that in the control group (P < 0.05).
27281873	0	114	theme	POROUS	65:70	arg1	SCAFFOLD					72:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD	23:79	[EXPERIMENTAL STUDY ON CHITOSAN/ALLOGENEIC BONE POWDER COMPOSITE POROUS SCAFFOLD TO REPAIR BONE DEFECTS IN RATS].
27281873	6	115	theme	condyle	816:822	arg1	hole					791:794	the hole	787:794	the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group)	787:901	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	6	116	theme	composite	717:725	arg1	scaffolds					734:742	The composite porous scaffolds	713:742	The composite porous scaffolds	713:742	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	9	117	with	bone	1511:1514	arg1	time					1521:1524	time	1521:1524	time	1521:1524	The gross observation and histological observation showed that the defect area was filled with new bone with time, and new bone of the experimental group was significantly more than that of the control group.
27281873	6	118	theme	left	803:806	arg1	condyle					816:822	the left femoral condyle	799:822	the left femoral condyle (experimental group)	799:843	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	6	118	theme	left	803:806	arg1	group					838:842	experimental group	825:842	experimental group	825:842	The composite porous scaffolds and chitosan scaffolds were implanted into the hole of the left femoral condyle (experimental group) and the hole of the right femoral condyle (control group), respectively.
27281873	9	119	theme	histological	1438:1449	arg1	observation					1451:1461	histological observation	1438:1461	histological observation	1438:1461	The gross observation and histological observation showed that the defect area was filled with new bone with time, and new bone of the experimental group was significantly more than that of the control group.
27281873	12	120	theme	osteogenic	2138:2147	arg1	activity					2149:2156	good osteogenic activity	2133:2156	good osteogenic activity	2133:2156	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	8	121	theme	significant	1293:1303	arg1	difference					1305:1314	no significant difference	1290:1314	no significant difference	1290:1314	RESULTS The composite porous scaffold prepared by vacuum freeze-drying technique had yellowish color, and brittle and easily broken texture; pore size was mostly 200-300 μm; and the porosity was 76.8% ± 1.1%, showing no significant difference when compared with the porosity of pure chitosan scaffold (78.4% ± 1.4%) (t = -2.10, P = 0.09).
27281873	1	122	theme	bone	236:239	arg1	engineering					248:258	bone tissue engineering	236:258	bone tissue engineering	236:258	OBJECTIVE To explore the feasibility of chitosan/allogeneic bone powder composite porous scaffold as scaffold material of bone tissue engineering in repairing bone defect.
27281873	12	123	theme	CONCLUSION	2038:2047	arg1	scaffold					2098:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold	2038:2105	CONCLUSION Chitosan/allogeneic bone powder composite porous scaffold has suitable porosity and good osteogenic activity, so it is a good material for repairing bone defect, and its bone forming volume and bone formation rate are better than those of pure chitosan scaffold.
27281873	1	124	theme	engineering	248:258	arg1	material					224:231	scaffold material	215:231	scaffold material of bone tissue engineering	215:258	OBJECTIVE To explore the feasibility of chitosan/allogeneic bone powder composite porous scaffold as scaffold material of bone tissue engineering in repairing bone defect.
28843118	9	0	theme	significant	1696:1706	arg1	effect					1717:1722	significant negative effect	1696:1722	significant negative effect on polymer technical properties	1696:1754	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	6	1	theme	films	1158:1162	arg1	permeability					1128:1139	water vapor permeability	1116:1139	water vapor permeability of the resulting films	1116:1162	Moreover, according to physical analysis, incorporation of 0.5% v/v ZME to the composite primary solutions improved water vapor permeability of the resulting films.
28843118	7	2	theme	active	1183:1188	arg1	films					1190:1194	the active films	1179:1194	the active films	1179:1194	Almost all of the active films were effective against the tested bacteria except for PLA/PEE films, and maximum antibacterial effects recorded for the films containing both ZME and PEE.
28843118	9	3	theme	polymer	1727:1733	arg1	properties					1745:1754	polymer technical properties	1727:1754	polymer technical properties	1727:1754	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	4	4	theme	antimicrobial	684:696	arg1	application					698:708	their antimicrobial application	678:708	their antimicrobial application	678:708	The resulting films were characterized by mechanical and physical tests and their antimicrobial application was evaluated in-vitro against four common foodborne pathogens and in vacuum-packed cooked sausages during refrigerated storage.
28843118	1	5	theme	food	217:220	arg1	materials					232:240	food packaging materials	217:240	food packaging materials	217:240	Biodegradability and antimicrobial activity of food packaging materials are among the most attractive parameters in modern food industries.
28843118	1	6	theme	materials	232:240	arg1	Biodegradability					170:185	Biodegradability	170:185	Biodegradability	170:185	Biodegradability and antimicrobial activity of food packaging materials are among the most attractive parameters in modern food industries.
28843118	1	6	theme	materials	232:240	arg1	activity					205:212	antimicrobial activity	191:212	antimicrobial activity	191:212	Biodegradability and antimicrobial activity of food packaging materials are among the most attractive parameters in modern food industries.
28843118	4	7	theme	physical	659:666	arg1	tests					668:672	mechanical and physical tests	644:672	mechanical and physical tests	644:672	The resulting films were characterized by mechanical and physical tests and their antimicrobial application was evaluated in-vitro against four common foodborne pathogens and in vacuum-packed cooked sausages during refrigerated storage.
28843118	0	8	theme	vacuum-packed	139:151	arg1	sausages					160:167	refrigerated vacuum-packed cooked sausages	126:167	refrigerated vacuum-packed cooked sausages	126:167	Antimicrobial evaluation of novel poly-lactic acid based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages.
28843118	3	9	theme	solvent	578:584	arg1	method					594:599	solvent casting method	578:599	solvent casting method	578:599	essential oil (ZME), propolis ethanolic extract (PEE) and cellulose nanofiber (CNF) were incorporated to the polymer by solvent casting method.
28843118	5	10	theme	made	900:903	arg1	films					905:909	ZME and PEE made films	888:909	ZME and PEE made films more flexible	888:923	Mechanical examination revealed that addition of ZME and PEE made films more flexible and incorporation of CNF improved almost all mechanical parameters tested.
28843118	6	11	theme	physical	1023:1030	arg1	analysis					1032:1039	physical analysis	1023:1039	physical analysis	1023:1039	Moreover, according to physical analysis, incorporation of 0.5% v/v ZME to the composite primary solutions improved water vapor permeability of the resulting films.
28843118	5	12	theme	ZME	888:890	arg1	films					905:909	ZME and PEE made films	888:909	ZME and PEE made films more flexible	888:923	Mechanical examination revealed that addition of ZME and PEE made films more flexible and incorporation of CNF improved almost all mechanical parameters tested.
28843118	4	13	theme	foodborne	753:761	arg1	pathogens					763:771	four common foodborne pathogens	741:771	four common foodborne pathogens	741:771	The resulting films were characterized by mechanical and physical tests and their antimicrobial application was evaluated in-vitro against four common foodborne pathogens and in vacuum-packed cooked sausages during refrigerated storage.
28843118	9	14	theme	natural	1536:1542	arg1	PEE					1585:1587	PEE	1585:1587	PEE	1585:1587	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	9	14	theme	natural	1536:1542	arg1	substances					1558:1567	natural antimicrobial substances	1536:1567	natural antimicrobial substances such as ZME and PEE	1536:1587	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	9	14	theme	natural	1536:1542	arg1	ZME					1577:1579	ZME	1577:1579	ZME	1577:1579	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	1	15	from	parameters	272:281	arg1	industries					298:307	modern food industries	286:307	modern food industries	286:307	Biodegradability and antimicrobial activity of food packaging materials are among the most attractive parameters in modern food industries.
28843118	9	16	theme	substances	1558:1567	arg1	approach					1635:1642	an interesting approach	1620:1642	an interesting approach in development of active packaging material	1620:1686	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	9	16	theme	substances	1558:1567	arg1	incorporation					1519:1531	incorporation	1519:1531	incorporation of natural antimicrobial substances such as ZME and PEE to packaging material	1519:1609	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	8	17	theme	microbiological	1364:1378	arg1	evaluation					1392:1401	the microbiological and sensory evaluation	1360:1401	the microbiological and sensory evaluation of the sausages	1360:1417	Based on the microbiological and sensory evaluation of the sausages, all of the PLA/1%ZME/PEE composites increased the shelf life to >40days.
28843118	6	18	theme	ZME	1068:1070	arg1	incorporation					1042:1054	incorporation	1042:1054	incorporation of 0.5% v/v ZME to the composite primary solutions	1042:1105	Moreover, according to physical analysis, incorporation of 0.5% v/v ZME to the composite primary solutions improved water vapor permeability of the resulting films.
28843118	8	19	theme	sensory	1384:1390	arg1	evaluation					1392:1401	the microbiological and sensory evaluation	1360:1401	the microbiological and sensory evaluation of the sausages	1360:1417	Based on the microbiological and sensory evaluation of the sausages, all of the PLA/1%ZME/PEE composites increased the shelf life to >40days.
28843118	0	20	theme	bioactive	90:98	arg1	compounds					100:108	bioactive compounds	90:108	bioactive compounds in-vitro	90:117	Antimicrobial evaluation of novel poly-lactic acid based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages.
28843118	6	21	theme	%	1062:1062	arg1	v/v					1064:1066	0.5% v/v	1059:1066	0.5% v/v ZME	1059:1070	Moreover, according to physical analysis, incorporation of 0.5% v/v ZME to the composite primary solutions improved water vapor permeability of the resulting films.
28843118	4	22	theme	refrigerated	817:828	arg1	storage					830:836	refrigerated storage	817:836	refrigerated storage	817:836	The resulting films were characterized by mechanical and physical tests and their antimicrobial application was evaluated in-vitro against four common foodborne pathogens and in vacuum-packed cooked sausages during refrigerated storage.
28843118	0	23	theme	in-vitro	110:117	arg1	compounds					100:108	bioactive compounds	90:108	bioactive compounds in-vitro	90:117	Antimicrobial evaluation of novel poly-lactic acid based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages.
28843118	8	24	theme	sausages	1410:1417	arg1	evaluation					1392:1401	the microbiological and sensory evaluation	1360:1401	the microbiological and sensory evaluation of the sausages	1360:1417	Based on the microbiological and sensory evaluation of the sausages, all of the PLA/1%ZME/PEE composites increased the shelf life to >40days.
28843118	2	25	theme	Bioss	451:455	arg1	concentration					415:427	different concentration	405:427	different concentration of Zataria multiflora Bioss	405:455	In order to develop biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites, different concentration of Zataria multiflora Bioss.
28843118	7	26	theme	PLA/PEE	1250:1256	arg1	films					1258:1262	PLA/PEE films	1250:1262	PLA/PEE films	1250:1262	Almost all of the active films were effective against the tested bacteria except for PLA/PEE films, and maximum antibacterial effects recorded for the films containing both ZME and PEE.
28843118	0	27	theme	acid	46:49	arg1	based					51:55	novel poly-lactic acid based	28:55	novel poly-lactic acid based	28:55	Antimicrobial evaluation of novel poly-lactic acid based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages.
28843118	4	28	theme	vacuum-packed	780:792	arg1	sausages					801:808	vacuum-packed cooked sausages	780:808	vacuum-packed cooked sausages during refrigerated storage	780:836	The resulting films were characterized by mechanical and physical tests and their antimicrobial application was evaluated in-vitro against four common foodborne pathogens and in vacuum-packed cooked sausages during refrigerated storage.
28843118	0	29	theme	Antimicrobial	0:12	arg1	evaluation					14:23	Antimicrobial evaluation	0:23	Antimicrobial evaluation of novel poly-lactic acid based	0:55	Antimicrobial evaluation of novel poly-lactic acid based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages.
28843118	6	30	theme	vapor	1122:1126	arg1	permeability					1128:1139	water vapor permeability	1116:1139	water vapor permeability of the resulting films	1116:1162	Moreover, according to physical analysis, incorporation of 0.5% v/v ZME to the composite primary solutions improved water vapor permeability of the resulting films.
28843118	7	31	theme	tested	1223:1228	arg1	bacteria					1230:1237	the tested bacteria	1219:1237	the tested bacteria	1219:1237	Almost all of the active films were effective against the tested bacteria except for PLA/PEE films, and maximum antibacterial effects recorded for the films containing both ZME and PEE.
28843118	9	32	theme	interesting	1623:1633	arg1	approach					1635:1642	an interesting approach	1620:1642	an interesting approach in development of active packaging material	1620:1686	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	9	32	theme	interesting	1623:1633	arg1	incorporation					1519:1531	incorporation	1519:1531	incorporation of natural antimicrobial substances such as ZME and PEE to packaging material	1519:1609	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	9	33	from	approach	1635:1642	arg1	development					1647:1657	development	1647:1657	development of active packaging material	1647:1686	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	8	34	theme	%	1436:1436	arg1	composites					1445:1454	the PLA/1%ZME/PEE composites	1427:1454	the PLA/1%ZME/PEE composites	1427:1454	Based on the microbiological and sensory evaluation of the sausages, all of the PLA/1%ZME/PEE composites increased the shelf life to >40days.
28843118	3	35	theme	ethanolic	488:496	arg1	extract					498:504	propolis ethanolic extract	479:504	propolis ethanolic extract (PEE)	479:510	essential oil (ZME), propolis ethanolic extract (PEE) and cellulose nanofiber (CNF) were incorporated to the polymer by solvent casting method.
28843118	3	35	theme	ethanolic	488:496	arg1	PEE					507:509	PEE	507:509	PEE	507:509	essential oil (ZME), propolis ethanolic extract (PEE) and cellulose nanofiber (CNF) were incorporated to the polymer by solvent casting method.
28843118	0	36	theme	poly-lactic	34:44	arg1	based					51:55	novel poly-lactic acid based	28:55	novel poly-lactic acid based	28:55	Antimicrobial evaluation of novel poly-lactic acid based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages.
28843118	6	37	theme	primary	1089:1095	arg1	solutions					1097:1105	the composite primary solutions	1075:1105	the composite primary solutions	1075:1105	Moreover, according to physical analysis, incorporation of 0.5% v/v ZME to the composite primary solutions improved water vapor permeability of the resulting films.
28843118	3	38	theme	cellulose	516:524	arg1	CNF					537:539	CNF	537:539	CNF	537:539	essential oil (ZME), propolis ethanolic extract (PEE) and cellulose nanofiber (CNF) were incorporated to the polymer by solvent casting method.
28843118	3	38	theme	cellulose	516:524	arg1	nanofiber					526:534	cellulose nanofiber	516:534	cellulose nanofiber (CNF)	516:540	essential oil (ZME), propolis ethanolic extract (PEE) and cellulose nanofiber (CNF) were incorporated to the polymer by solvent casting method.
28843118	0	39	theme	based	51:55	arg1	evaluation					14:23	Antimicrobial evaluation	0:23	Antimicrobial evaluation of novel poly-lactic acid based	0:55	Antimicrobial evaluation of novel poly-lactic acid based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages.
28843118	1	40	theme	attractive	261:270	arg1	parameters					272:281	the most attractive parameters	252:281	the most attractive parameters in modern food industries	252:307	Biodegradability and antimicrobial activity of food packaging materials are among the most attractive parameters in modern food industries.
28843118	9	41	theme	packaging	1669:1677	arg1	material					1679:1686	active packaging material	1662:1686	active packaging material	1662:1686	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	1	42	theme	food	293:296	arg1	industries					298:307	modern food industries	286:307	modern food industries	286:307	Biodegradability and antimicrobial activity of food packaging materials are among the most attractive parameters in modern food industries.
28843118	9	43	theme	negative	1708:1715	arg1	effect					1717:1722	significant negative effect	1696:1722	significant negative effect on polymer technical properties	1696:1754	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	6	44	theme	resulting	1148:1156	arg1	films					1158:1162	the resulting films	1144:1162	the resulting films	1144:1162	Moreover, according to physical analysis, incorporation of 0.5% v/v ZME to the composite primary solutions improved water vapor permeability of the resulting films.
28843118	2	45	theme	biodegradable	330:342	arg1	film					367:370	biodegradable poly-lactic acid (PLA) film	330:370	biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites	330:402	In order to develop biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites, different concentration of Zataria multiflora Bioss.
28843118	4	46	theme	resulting	606:614	arg1	films					616:620	The resulting films	602:620	The resulting films	602:620	The resulting films were characterized by mechanical and physical tests and their antimicrobial application was evaluated in-vitro against four common foodborne pathogens and in vacuum-packed cooked sausages during refrigerated storage.
28843118	2	47	theme	antibacterial	375:387	arg1	nanocomposites					389:402	antibacterial nanocomposites	375:402	antibacterial nanocomposites	375:402	In order to develop biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites, different concentration of Zataria multiflora Bioss.
28843118	5	48	theme	flexible	916:923	arg1	films					905:909	ZME and PEE made films	888:909	ZME and PEE made films more flexible	888:923	Mechanical examination revealed that addition of ZME and PEE made films more flexible and incorporation of CNF improved almost all mechanical parameters tested.
28843118	9	49	theme	technical	1735:1743	arg1	properties					1745:1754	polymer technical properties	1727:1754	polymer technical properties	1727:1754	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	7	50	theme	maximum	1269:1275	arg1	effects					1291:1297	maximum antibacterial effects	1269:1297	maximum antibacterial effects	1269:1297	Almost all of the active films were effective against the tested bacteria except for PLA/PEE films, and maximum antibacterial effects recorded for the films containing both ZME and PEE.
28843118	3	51	theme	casting	586:592	arg1	method					594:599	solvent casting method	578:599	solvent casting method	578:599	essential oil (ZME), propolis ethanolic extract (PEE) and cellulose nanofiber (CNF) were incorporated to the polymer by solvent casting method.
28843118	1	52	theme	packaging	222:230	arg1	materials					232:240	food packaging materials	217:240	food packaging materials	217:240	Biodegradability and antimicrobial activity of food packaging materials are among the most attractive parameters in modern food industries.
28843118	0	53	theme	cooked	153:158	arg1	sausages					160:167	refrigerated vacuum-packed cooked sausages	126:167	refrigerated vacuum-packed cooked sausages	126:167	Antimicrobial evaluation of novel poly-lactic acid based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages.
28843118	2	54	theme	acid	356:359	arg1	film					367:370	biodegradable poly-lactic acid (PLA) film	330:370	biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites	330:402	In order to develop biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites, different concentration of Zataria multiflora Bioss.
28843118	5	55	theme	films	905:909	arg1	incorporation					929:941	incorporation	929:941	incorporation of CNF	929:948	Mechanical examination revealed that addition of ZME and PEE made films more flexible and incorporation of CNF improved almost all mechanical parameters tested.
28843118	5	55	theme	films	905:909	arg1	addition					876:883	addition	876:883	addition of ZME and PEE made films more flexible	876:923	Mechanical examination revealed that addition of ZME and PEE made films more flexible and incorporation of CNF improved almost all mechanical parameters tested.
28843118	9	56	from	effect	1717:1722	arg1	properties					1745:1754	polymer technical properties	1727:1754	polymer technical properties	1727:1754	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	5	57	theme	mechanical	970:979	arg1	parameters					981:990	almost all mechanical parameters	959:990	almost all mechanical parameters tested	959:997	Mechanical examination revealed that addition of ZME and PEE made films more flexible and incorporation of CNF improved almost all mechanical parameters tested.
28843118	4	58	theme	common	746:751	arg1	pathogens					763:771	four common foodborne pathogens	741:771	four common foodborne pathogens	741:771	The resulting films were characterized by mechanical and physical tests and their antimicrobial application was evaluated in-vitro against four common foodborne pathogens and in vacuum-packed cooked sausages during refrigerated storage.
28843118	9	59	theme	antimicrobial	1544:1556	arg1	PEE					1585:1587	PEE	1585:1587	PEE	1585:1587	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	9	59	theme	antimicrobial	1544:1556	arg1	substances					1558:1567	natural antimicrobial substances	1536:1567	natural antimicrobial substances such as ZME and PEE	1536:1587	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	9	59	theme	antimicrobial	1544:1556	arg1	ZME					1577:1579	ZME	1577:1579	ZME	1577:1579	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	6	60	theme	composite	1079:1087	arg1	solutions					1097:1105	the composite primary solutions	1075:1105	the composite primary solutions	1075:1105	Moreover, according to physical analysis, incorporation of 0.5% v/v ZME to the composite primary solutions improved water vapor permeability of the resulting films.
28843118	7	61	theme	antibacterial	1277:1289	arg1	effects					1291:1297	maximum antibacterial effects	1269:1297	maximum antibacterial effects	1269:1297	Almost all of the active films were effective against the tested bacteria except for PLA/PEE films, and maximum antibacterial effects recorded for the films containing both ZME and PEE.
28843118	5	62	theme	CNF	946:948	arg1	incorporation					929:941	incorporation	929:941	incorporation of CNF	929:948	Mechanical examination revealed that addition of ZME and PEE made films more flexible and incorporation of CNF improved almost all mechanical parameters tested.
28843118	5	62	theme	CNF	946:948	arg1	addition					876:883	addition	876:883	addition of ZME and PEE made films more flexible	876:923	Mechanical examination revealed that addition of ZME and PEE made films more flexible and incorporation of CNF improved almost all mechanical parameters tested.
28843118	1	63	theme	antimicrobial	191:203	arg1	activity					205:212	antimicrobial activity	191:212	antimicrobial activity	191:212	Biodegradability and antimicrobial activity of food packaging materials are among the most attractive parameters in modern food industries.
28843118	6	64	theme	v/v	1064:1066	arg1	ZME					1068:1070	0.5% v/v ZME	1059:1070	0.5% v/v ZME	1059:1070	Moreover, according to physical analysis, incorporation of 0.5% v/v ZME to the composite primary solutions improved water vapor permeability of the resulting films.
28843118	2	65	dep	develop	322:328	arg1	concentration					415:427	different concentration	405:427	different concentration of Zataria multiflora Bioss	405:455	In order to develop biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites, different concentration of Zataria multiflora Bioss.
28843118	2	66	theme	poly-lactic	344:354	arg1	acid					356:359	poly-lactic acid	344:359	biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites	330:402	In order to develop biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites, different concentration of Zataria multiflora Bioss.
28843118	2	66	theme	poly-lactic	344:354	arg1	PLA					362:364	PLA	362:364	PLA	362:364	In order to develop biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites, different concentration of Zataria multiflora Bioss.
28843118	6	67	theme	0.5	1059:1061	arg1	%					1062:1062	%	1062:1062	%	1062:1062	Moreover, according to physical analysis, incorporation of 0.5% v/v ZME to the composite primary solutions improved water vapor permeability of the resulting films.
28843118	0	68	theme	refrigerated	126:137	arg1	sausages					160:167	refrigerated vacuum-packed cooked sausages	126:167	refrigerated vacuum-packed cooked sausages	126:167	Antimicrobial evaluation of novel poly-lactic acid based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages.
28843118	7	69	contain	containing	1322:1331	arg2	PEE					1346:1348	PEE	1346:1348	PEE	1346:1348	Almost all of the active films were effective against the tested bacteria except for PLA/PEE films, and maximum antibacterial effects recorded for the films containing both ZME and PEE.
28843118	7	69	contain	containing	1322:1331	arg2	ZME					1338:1340	ZME	1338:1340	ZME	1338:1340	Almost all of the active films were effective against the tested bacteria except for PLA/PEE films, and maximum antibacterial effects recorded for the films containing both ZME and PEE.
28843118	7	69	contain	containing	1322:1331	arg1	films					1316:1320	the films	1312:1320	the films containing both ZME and PEE	1312:1348	Almost all of the active films were effective against the tested bacteria except for PLA/PEE films, and maximum antibacterial effects recorded for the films containing both ZME and PEE.
28843118	9	70	theme	packaging	1592:1600	arg1	material					1602:1609	packaging material	1592:1609	packaging material	1592:1609	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	3	71	theme	propolis	479:486	arg1	extract					498:504	propolis ethanolic extract	479:504	propolis ethanolic extract (PEE)	479:510	essential oil (ZME), propolis ethanolic extract (PEE) and cellulose nanofiber (CNF) were incorporated to the polymer by solvent casting method.
28843118	3	71	theme	propolis	479:486	arg1	PEE					507:509	PEE	507:509	PEE	507:509	essential oil (ZME), propolis ethanolic extract (PEE) and cellulose nanofiber (CNF) were incorporated to the polymer by solvent casting method.
28843118	0	72	theme	novel	28:32	arg1	based					51:55	novel poly-lactic acid based	28:55	novel poly-lactic acid based	28:55	Antimicrobial evaluation of novel poly-lactic acid based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages.
28843118	6	73	theme	water	1116:1120	arg1	permeability					1128:1139	water vapor permeability	1116:1139	water vapor permeability of the resulting films	1116:1162	Moreover, according to physical analysis, incorporation of 0.5% v/v ZME to the composite primary solutions improved water vapor permeability of the resulting films.
28843118	4	74	theme	cooked	794:799	arg1	sausages					801:808	vacuum-packed cooked sausages	780:808	vacuum-packed cooked sausages during refrigerated storage	780:836	The resulting films were characterized by mechanical and physical tests and their antimicrobial application was evaluated in-vitro against four common foodborne pathogens and in vacuum-packed cooked sausages during refrigerated storage.
28843118	5	75	theme	Mechanical	839:848	arg1	examination					850:860	Mechanical examination	839:860	Mechanical examination	839:860	Mechanical examination revealed that addition of ZME and PEE made films more flexible and incorporation of CNF improved almost all mechanical parameters tested.
28843118	0	76	dep	nanocomposites	57:70	arg1	incorporated					72:83	incorporated	72:83	nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages	57:167	Antimicrobial evaluation of novel poly-lactic acid based nanocomposites incorporated with bioactive compounds in-vitro and in refrigerated vacuum-packed cooked sausages.
28843118	8	77	theme	PLA/1	1431:1435	arg1	%					1436:1436	%	1436:1436	%	1436:1436	Based on the microbiological and sensory evaluation of the sausages, all of the PLA/1%ZME/PEE composites increased the shelf life to >40days.
28843118	3	78	theme	essential	458:466	arg1	ZME					473:475	ZME	473:475	ZME	473:475	essential oil (ZME), propolis ethanolic extract (PEE) and cellulose nanofiber (CNF) were incorporated to the polymer by solvent casting method.
28843118	3	78	theme	essential	458:466	arg1	oil					468:470	essential oil	458:470	essential oil (ZME)	458:476	essential oil (ZME), propolis ethanolic extract (PEE) and cellulose nanofiber (CNF) were incorporated to the polymer by solvent casting method.
28843118	8	79	theme	ZME/PEE	1437:1443	arg1	composites					1445:1454	the PLA/1%ZME/PEE composites	1427:1454	the PLA/1%ZME/PEE composites	1427:1454	Based on the microbiological and sensory evaluation of the sausages, all of the PLA/1%ZME/PEE composites increased the shelf life to >40days.
28843118	9	80	theme	active	1662:1667	arg1	material					1679:1686	active packaging material	1662:1686	active packaging material	1662:1686	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	9	81	theme	material	1679:1686	arg1	development					1647:1657	development	1647:1657	development of active packaging material	1647:1686	The results indicate that incorporation of natural antimicrobial substances such as ZME and PEE to packaging material could be an interesting approach in development of active packaging material without significant negative effect on polymer technical properties.
28843118	4	82	theme	mechanical	644:653	arg1	tests					668:672	mechanical and physical tests	644:672	mechanical and physical tests	644:672	The resulting films were characterized by mechanical and physical tests and their antimicrobial application was evaluated in-vitro against four common foodborne pathogens and in vacuum-packed cooked sausages during refrigerated storage.
28843118	8	83	theme	shelf	1470:1474	arg1	life					1476:1479	the shelf life	1466:1479	the shelf life	1466:1479	Based on the microbiological and sensory evaluation of the sausages, all of the PLA/1%ZME/PEE composites increased the shelf life to >40days.
28843118	2	84	theme	different	405:413	arg1	concentration					415:427	different concentration	405:427	different concentration of Zataria multiflora Bioss	405:455	In order to develop biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites, different concentration of Zataria multiflora Bioss.
28843118	1	85	theme	modern	286:291	arg1	industries					298:307	modern food industries	286:307	modern food industries	286:307	Biodegradability and antimicrobial activity of food packaging materials are among the most attractive parameters in modern food industries.
26687036	4	0	theme	glycosylated	540:551	arg1	proteins					553:560	glycosylated proteins	540:560	glycosylated proteins	540:560	Despite their prevalence, the complete inventory and biological significance of glycosylated proteins in Toxoplasma remain unknown.
26687036	1	1	from	types	200:204	arg1	organisms					230:238	diverse warm-blooded organisms	209:238	diverse warm-blooded organisms	209:238	Toxoplasma gondii is an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms.
26687036	10	2	theme	unique	1388:1393	arg1	proteins					1395:1402	89 unique proteins	1385:1402	89 unique proteins	1385:1402	Subsequent mass spectrometry analysis revealed 89 unique proteins.
26687036	4	3	dep	inventory	499:507	arg1	the					486:488	the	486:488	the	486:488	Despite their prevalence, the complete inventory and biological significance of glycosylated proteins in Toxoplasma remain unknown.
26687036	0	4	theme	cellular	93:100	arg1	proteins					102:109	cellular proteins	93:109	cellular proteins	93:109	Extracellular Toxoplasma gondii tachyzoites metabolize and incorporate unnatural sugars into cellular proteins.
26687036	6	5	with	labeling	866:873	arg1	probes					887:892	visual probes	880:892	visual probes	880:892	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	6	5	with	labeling	866:873	arg1	tags					906:909	affinity tags	897:909	affinity tags	897:909	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	5	6	theme	parasite	636:643	arg1	glycoproteins					645:657	parasite glycoproteins	636:657	parasite glycoproteins using a bioorthogonal chemical reporter strategy	636:706	In this study, we aimed to globally profile parasite glycoproteins using a bioorthogonal chemical reporter strategy.
26687036	1	7	theme	nucleated	185:193	arg1	types					200:204	all nucleated cell types	181:204	all nucleated cell types in diverse warm-blooded organisms	181:238	Toxoplasma gondii is an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms.
26687036	7	8	dep	visualization	946:958	arg1	the					942:944	the	942:944	the	942:944	The two-step approach enables the visualization and identification of newly biosynthesized glycoconjugates in the parasite.
26687036	9	9	theme	labeled	1318:1324	arg1	structures					1326:1335	these labeled structures	1312:1335	these labeled structures	1312:1335	Covalent chemistries were used to visualize and retrieve these labeled structures.
26687036	8	10	theme	intracellular	1063:1075	arg1	conditions					1077:1086	intracellular conditions	1063:1086	intracellular conditions	1063:1086	Using a buffer that mimics intracellular conditions, extracellular Toxoplasma tachyzoites were found to metabolize and incorporate unnatural sugars (equipped with bioorthogonal functional groups) into diverse proteins.
26687036	11	11	theme	novel	1428:1432	arg1	proteins					1434:1441	novel proteins	1428:1441	novel proteins as well as previously characterized proteins from lectin affinity analyses	1428:1516	This survey identified novel proteins as well as previously characterized proteins from lectin affinity analyses.
26687036	9	12	theme	Covalent	1255:1262	arg1	chemistries					1264:1274	Covalent chemistries	1255:1274	Covalent chemistries	1255:1274	Covalent chemistries were used to visualize and retrieve these labeled structures.
26687036	4	13	theme	biological	513:522	arg1	significance					524:535	biological significance	513:535	biological significance	513:535	Despite their prevalence, the complete inventory and biological significance of glycosylated proteins in Toxoplasma remain unknown.
26687036	6	14	theme	covalent	857:864	arg1	labeling					866:873	covalent labeling	857:873	covalent labeling with visual probes or affinity tags	857:909	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	11	15	from	analyses	1509:1516	arg1	proteins					1434:1441	novel proteins	1428:1441	novel proteins as well as previously characterized proteins from lectin affinity analyses	1428:1516	This survey identified novel proteins as well as previously characterized proteins from lectin affinity analyses.
26687036	11	15	from	analyses	1509:1516	arg1	proteins					1479:1486	previously characterized proteins	1454:1486	novel proteins as well as previously characterized proteins from lectin affinity analyses	1428:1516	This survey identified novel proteins as well as previously characterized proteins from lectin affinity analyses.
26687036	4	16	gly	glycosylated	540:551	arg1	proteins					553:560	glycosylated proteins	540:560	glycosylated proteins	540:560	Despite their prevalence, the complete inventory and biological significance of glycosylated proteins in Toxoplasma remain unknown.
26687036	5	17	theme	bioorthogonal	667:679	arg1	strategy					699:706	a bioorthogonal chemical reporter strategy	665:706	a bioorthogonal chemical reporter strategy	665:706	In this study, we aimed to globally profile parasite glycoproteins using a bioorthogonal chemical reporter strategy.
26687036	6	18	theme	groups	784:789	arg1	incorporation					746:758	the metabolic incorporation	732:758	the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans	732:842	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	1	19	theme	cell	195:198	arg1	types					200:204	all nucleated cell types	181:204	all nucleated cell types in diverse warm-blooded organisms	181:238	Toxoplasma gondii is an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms.
26687036	3	20	theme	Glycosylated	384:395	arg1	proteins					397:404	Glycosylated proteins	384:404	Glycosylated proteins in the nucleus and cytoplasm	384:433	Glycosylated proteins in the nucleus and cytoplasm have also been reported.
26687036	4	21	from	significance	524:535	arg1	Toxoplasma					565:574	Toxoplasma	565:574	Toxoplasma	565:574	Despite their prevalence, the complete inventory and biological significance of glycosylated proteins in Toxoplasma remain unknown.
26687036	7	22	from	visualization	946:958	arg1	parasite					1026:1033	the parasite	1022:1033	the parasite	1022:1033	The two-step approach enables the visualization and identification of newly biosynthesized glycoconjugates in the parasite.
26687036	6	23	theme	functional	773:782	arg1	groups					784:789	unnatural functional groups	763:789	unnatural functional groups (i.e., "chemical reporters")	763:818	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	0	24	theme	Toxoplasma	14:23	arg1	tachyzoites					32:42	Extracellular Toxoplasma gondii tachyzoites	0:42	Extracellular Toxoplasma gondii tachyzoites	0:42	Extracellular Toxoplasma gondii tachyzoites metabolize and incorporate unnatural sugars into cellular proteins.
26687036	5	25	theme	reporter	690:697	arg1	strategy					699:706	a bioorthogonal chemical reporter strategy	665:706	a bioorthogonal chemical reporter strategy	665:706	In this study, we aimed to globally profile parasite glycoproteins using a bioorthogonal chemical reporter strategy.
26687036	7	26	theme	glycoconjugates	1003:1017	arg1	identification					964:977	identification	964:977	identification	964:977	The two-step approach enables the visualization and identification of newly biosynthesized glycoconjugates in the parasite.
26687036	7	26	theme	glycoconjugates	1003:1017	arg1	visualization					946:958	visualization	946:958	visualization	946:958	The two-step approach enables the visualization and identification of newly biosynthesized glycoconjugates in the parasite.
26687036	6	27	theme	unnatural	763:771	arg1	groups					784:789	unnatural functional groups	763:789	unnatural functional groups (i.e., "chemical reporters")	763:818	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	11	28	theme	affinity	1500:1507	arg1	analyses					1509:1516	lectin affinity analyses	1493:1516	lectin affinity analyses	1493:1516	This survey identified novel proteins as well as previously characterized proteins from lectin affinity analyses.
26687036	0	29	theme	Extracellular	0:12	arg1	tachyzoites					32:42	Extracellular Toxoplasma gondii tachyzoites	0:42	Extracellular Toxoplasma gondii tachyzoites	0:42	Extracellular Toxoplasma gondii tachyzoites metabolize and incorporate unnatural sugars into cellular proteins.
26687036	7	30	theme	biosynthesized	988:1001	arg1	glycoconjugates					1003:1017	newly biosynthesized glycoconjugates	982:1017	newly biosynthesized glycoconjugates	982:1017	The two-step approach enables the visualization and identification of newly biosynthesized glycoconjugates in the parasite.
26687036	2	31	theme	intracellular	338:350	arg1	growth					352:357	intracellular growth	338:357	intracellular growth	338:357	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	1	32	theme	diverse	209:215	arg1	organisms					230:238	diverse warm-blooded organisms	209:238	diverse warm-blooded organisms	209:238	Toxoplasma gondii is an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms.
26687036	8	33	theme	Toxoplasma	1103:1112	arg1	tachyzoites					1114:1124	extracellular Toxoplasma tachyzoites	1089:1124	extracellular Toxoplasma tachyzoites	1089:1124	Using a buffer that mimics intracellular conditions, extracellular Toxoplasma tachyzoites were found to metabolize and incorporate unnatural sugars (equipped with bioorthogonal functional groups) into diverse proteins.
26687036	4	34	from	inventory	499:507	arg1	Toxoplasma					565:574	Toxoplasma	565:574	Toxoplasma	565:574	Despite their prevalence, the complete inventory and biological significance of glycosylated proteins in Toxoplasma remain unknown.
26687036	6	35	theme	Toxoplasma	825:834	arg1	glycans					836:842	Toxoplasma glycans	825:842	Toxoplasma glycans	825:842	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	8	36	theme	extracellular	1089:1101	arg1	tachyzoites					1114:1124	extracellular Toxoplasma tachyzoites	1089:1124	extracellular Toxoplasma tachyzoites	1089:1124	Using a buffer that mimics intracellular conditions, extracellular Toxoplasma tachyzoites were found to metabolize and incorporate unnatural sugars (equipped with bioorthogonal functional groups) into diverse proteins.
26687036	1	37	theme	warm-blooded	217:228	arg1	organisms					230:238	diverse warm-blooded organisms	209:238	diverse warm-blooded organisms	209:238	Toxoplasma gondii is an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms.
26687036	1	38	theme	Toxoplasma	112:121	arg1	parasite					159:166	an obligate intracellular parasite	133:166	an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms	133:238	Toxoplasma gondii is an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms.
26687036	1	38	theme	Toxoplasma	112:121	arg1	gondii					123:128	Toxoplasma gondii	112:128	Toxoplasma gondii	112:128	Toxoplasma gondii is an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms.
26687036	6	39	theme	metabolic	736:744	arg1	incorporation					746:758	the metabolic incorporation	732:758	the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans	732:842	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	7	40	from	identification	964:977	arg1	parasite					1026:1033	the parasite	1022:1033	the parasite	1022:1033	The two-step approach enables the visualization and identification of newly biosynthesized glycoconjugates in the parasite.
26687036	7	41	theme	two-step	916:923	arg1	approach					925:932	The two-step approach	912:932	The two-step approach	912:932	The two-step approach enables the visualization and identification of newly biosynthesized glycoconjugates in the parasite.
26687036	9	42	used	used	1281:1284	arg2	chemistries					1264:1274	Covalent chemistries	1255:1274	Covalent chemistries	1255:1274	Covalent chemistries were used to visualize and retrieve these labeled structures.
26687036	2	43	theme	antigens	261:268	arg1	antigens					261:268	surface antigens	253:268	surface antigens	253:268	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	2	43	theme	antigens	261:268	arg1	molecules					283:291	effector molecules	274:291	effector molecules	274:291	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	2	43	theme	antigens	261:268	arg1	Many					241:244	Many	241:244	Many	241:244	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	10	44	theme	Subsequent	1338:1347	arg1	analysis					1367:1374	Subsequent mass spectrometry analysis	1338:1374	Subsequent mass spectrometry analysis	1338:1374	Subsequent mass spectrometry analysis revealed 89 unique proteins.
26687036	2	45	dep	antigens	261:268	arg1	the					249:251	the	249:251	the	249:251	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	3	46	from	proteins	397:404	arg1	cytoplasm					425:433	cytoplasm	425:433	cytoplasm	425:433	Glycosylated proteins in the nucleus and cytoplasm have also been reported.
26687036	3	46	from	proteins	397:404	arg1	nucleus					413:419	nucleus	413:419	nucleus	413:419	Glycosylated proteins in the nucleus and cytoplasm have also been reported.
26687036	2	47	theme	surface	253:259	arg1	antigens					261:268	surface antigens	253:268	surface antigens	253:268	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	0	48	theme	unnatural	71:79	arg1	sugars					81:86	unnatural sugars	71:86	unnatural sugars	71:86	Extracellular Toxoplasma gondii tachyzoites metabolize and incorporate unnatural sugars into cellular proteins.
26687036	8	49	theme	functional	1213:1222	arg1	groups					1224:1229	bioorthogonal functional groups	1199:1229	bioorthogonal functional groups	1199:1229	Using a buffer that mimics intracellular conditions, extracellular Toxoplasma tachyzoites were found to metabolize and incorporate unnatural sugars (equipped with bioorthogonal functional groups) into diverse proteins.
26687036	4	50	theme	complete	490:497	arg1	inventory					499:507	complete inventory	490:507	complete inventory	490:507	Despite their prevalence, the complete inventory and biological significance of glycosylated proteins in Toxoplasma remain unknown.
26687036	11	51	theme	lectin	1493:1498	arg1	analyses					1509:1516	lectin affinity analyses	1493:1516	lectin affinity analyses	1493:1516	This survey identified novel proteins as well as previously characterized proteins from lectin affinity analyses.
26687036	8	52	theme	bioorthogonal	1199:1211	arg1	groups					1224:1229	bioorthogonal functional groups	1199:1229	bioorthogonal functional groups	1199:1229	Using a buffer that mimics intracellular conditions, extracellular Toxoplasma tachyzoites were found to metabolize and incorporate unnatural sugars (equipped with bioorthogonal functional groups) into diverse proteins.
26687036	6	53	theme	affinity	897:904	arg1	tags					906:909	affinity tags	897:909	affinity tags	897:909	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	5	54	theme	chemical	681:688	arg1	strategy					699:706	a bioorthogonal chemical reporter strategy	665:706	a bioorthogonal chemical reporter strategy	665:706	In this study, we aimed to globally profile parasite glycoproteins using a bioorthogonal chemical reporter strategy.
26687036	1	55	theme	obligate	136:143	arg1	parasite					159:166	an obligate intracellular parasite	133:166	an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms	133:238	Toxoplasma gondii is an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms.
26687036	1	55	theme	obligate	136:143	arg1	gondii					123:128	Toxoplasma gondii	112:128	Toxoplasma gondii	112:128	Toxoplasma gondii is an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms.
26687036	8	56	theme	unnatural	1167:1175	arg1	sugars					1177:1182	unnatural sugars	1167:1182	unnatural sugars (equipped with bioorthogonal functional groups)	1167:1230	Using a buffer that mimics intracellular conditions, extracellular Toxoplasma tachyzoites were found to metabolize and incorporate unnatural sugars (equipped with bioorthogonal functional groups) into diverse proteins.
26687036	6	57	dep	groups	784:789	arg1	"					817:817	chemical reporters"	799:817	"chemical reporters"	798:817	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	6	58	theme	reporters	808:816	arg1	"					817:817	chemical reporters"	799:817	"chemical reporters"	798:817	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	1	59	theme	intracellular	145:157	arg1	parasite					159:166	an obligate intracellular parasite	133:166	an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms	133:238	Toxoplasma gondii is an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms.
26687036	1	59	theme	intracellular	145:157	arg1	gondii					123:128	Toxoplasma gondii	112:128	Toxoplasma gondii	112:128	Toxoplasma gondii is an obligate intracellular parasite that infects all nucleated cell types in diverse warm-blooded organisms.
26687036	2	60	mod	modified	363:370	arg1	antigens					261:268	surface antigens	253:268	surface antigens	253:268	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	2	60	mod	modified	363:370	arg1	molecules					283:291	effector molecules	274:291	effector molecules	274:291	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	2	60	mod	modified	363:370	arg3	glycans					375:381	glycans	375:381	glycans	375:381	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	2	60	mod	modified	363:370	arg1	Many					241:244	Many	241:244	Many	241:244	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	5	61	gly	glycoproteins	645:657	arg1	glycoproteins					645:657	parasite glycoproteins	636:657	parasite glycoproteins using a bioorthogonal chemical reporter strategy	636:706	In this study, we aimed to globally profile parasite glycoproteins using a bioorthogonal chemical reporter strategy.
26687036	2	62	theme	molecules	283:291	arg1	antigens					261:268	surface antigens	253:268	surface antigens	253:268	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	2	62	theme	molecules	283:291	arg1	molecules					283:291	effector molecules	274:291	effector molecules	274:291	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	2	62	theme	molecules	283:291	arg1	Many					241:244	Many	241:244	Many	241:244	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	6	63	theme	chemical	799:806	arg1	"					817:817	chemical reporters"	799:817	"chemical reporters"	798:817	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	10	64	theme	mass	1349:1352	arg1	analysis					1367:1374	Subsequent mass spectrometry analysis	1338:1374	Subsequent mass spectrometry analysis	1338:1374	Subsequent mass spectrometry analysis revealed 89 unique proteins.
26687036	8	65	theme	diverse	1237:1243	arg1	proteins					1245:1252	diverse proteins	1237:1252	diverse proteins	1237:1252	Using a buffer that mimics intracellular conditions, extracellular Toxoplasma tachyzoites were found to metabolize and incorporate unnatural sugars (equipped with bioorthogonal functional groups) into diverse proteins.
26687036	6	66	theme	visual	880:885	arg1	probes					887:892	visual probes	880:892	visual probes	880:892	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
26687036	2	67	theme	effector	274:281	arg1	molecules					283:291	effector molecules	274:291	effector molecules	274:291	Many of the surface antigens and effector molecules secreted by the parasite during invasion and intracellular growth are modified by glycans.
26687036	10	68	theme	spectrometry	1354:1365	arg1	analysis					1367:1374	Subsequent mass spectrometry analysis	1338:1374	Subsequent mass spectrometry analysis	1338:1374	Subsequent mass spectrometry analysis revealed 89 unique proteins.
26687036	4	69	theme	proteins	553:560	arg1	significance					524:535	biological significance	513:535	biological significance	513:535	Despite their prevalence, the complete inventory and biological significance of glycosylated proteins in Toxoplasma remain unknown.
26687036	4	69	theme	proteins	553:560	arg1	inventory					499:507	complete inventory	490:507	complete inventory	490:507	Despite their prevalence, the complete inventory and biological significance of glycosylated proteins in Toxoplasma remain unknown.
26687036	0	70	theme	gondii	25:30	arg1	tachyzoites					32:42	Extracellular Toxoplasma gondii tachyzoites	0:42	Extracellular Toxoplasma gondii tachyzoites	0:42	Extracellular Toxoplasma gondii tachyzoites metabolize and incorporate unnatural sugars into cellular proteins.
26687036	11	71	theme	characterized	1465:1477	arg1	proteins					1479:1486	previously characterized proteins	1454:1486	novel proteins as well as previously characterized proteins from lectin affinity analyses	1428:1516	This survey identified novel proteins as well as previously characterized proteins from lectin affinity analyses.
26687036	6	72	dep	"	817:817	arg1	i.e.					792:795	i.e.	792:795	i.e.	792:795	This strategy involves the metabolic incorporation of unnatural functional groups (i.e., "chemical reporters") into Toxoplasma glycans, followed by covalent labeling with visual probes or affinity tags.
27474565	0	0	theme	drug	104:107	arg1	release					120:126	drug controlled release	104:126	drug controlled release	104:126	Multi-responsive magnetic microsphere of poly(N-isopropylacrylamide)/carboxymethylchitosan hydrogel for drug controlled release.
27474565	1	1	theme	nanoparticles	354:366	arg1	presence					300:307	the presence	296:307	the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs)	296:373	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	5	2	theme	controlled	1103:1112	arg1	applications					1122:1133	controlled release applications	1103:1133	controlled release applications	1103:1133	These responsive properties can be used as triggering mechanisms for releases of the entrapped drugs from the microspheres, indicating their great potentials for use in controlled release applications.
27474565	1	3	theme	in	193:194	arg1	polymerization					214:227	an in situ free radical polymerization	190:227	an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm))	190:291	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	2	4	theme	size	525:528	arg1	distributions					530:542	narrow size distributions	518:542	narrow size distributions	518:542	Formulation conditions of the composite microspheres were tuned such that spherical microspheres with narrow size distributions were obtained (30.0±1.0μm in diameter).
27474565	5	5	theme	great	1075:1079	arg1	potentials					1081:1090	their great potentials	1069:1090	their great potentials for use in controlled release applications	1069:1133	These responsive properties can be used as triggering mechanisms for releases of the entrapped drugs from the microspheres, indicating their great potentials for use in controlled release applications.
27474565	5	6	from	use	1096:1098	arg1	applications					1122:1133	controlled release applications	1103:1133	controlled release applications	1103:1133	These responsive properties can be used as triggering mechanisms for releases of the entrapped drugs from the microspheres, indicating their great potentials for use in controlled release applications.
27474565	5	7	theme	entrapped	1019:1027	arg1	drugs					1029:1033	the entrapped drugs	1015:1033	the entrapped drugs	1015:1033	These responsive properties can be used as triggering mechanisms for releases of the entrapped drugs from the microspheres, indicating their great potentials for use in controlled release applications.
27474565	0	8	theme	controlled	109:118	arg1	release					120:126	drug controlled release	104:126	drug controlled release	104:126	Multi-responsive magnetic microsphere of poly(N-isopropylacrylamide)/carboxymethylchitosan hydrogel for drug controlled release.
27474565	4	9	theme	solution	889:896	arg1	pH					898:899	the solution pH	885:899	the solution pH	885:899	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	1	10	dep	synthesized	174:184	arg1	followed					375:382	followed	375:382	followed by glutaraldehyde crosslinking	375:413	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	4	11	theme	indomethacin	743:754	arg1	drug					762:765	an entrapped indomethacin model drug	730:765	an entrapped indomethacin model drug	730:765	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	2	12	theme	composite	446:454	arg1	microspheres					456:467	the composite microspheres	442:467	the composite microspheres	442:467	Formulation conditions of the composite microspheres were tuned such that spherical microspheres with narrow size distributions were obtained (30.0±1.0μm in diameter).
27474565	1	13	theme	free	201:204	arg1	polymerization					214:227	an in situ free radical polymerization	190:227	an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm))	190:291	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	1	14	theme	radical	206:212	arg1	polymerization					214:227	an in situ free radical polymerization	190:227	an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm))	190:291	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	0	15	theme	magnetic	17:24	arg1	microsphere					26:36	Multi-responsive magnetic microsphere	0:36	Multi-responsive magnetic microsphere of poly(N-isopropylacrylamide)	0:67	Multi-responsive magnetic microsphere of poly(N-isopropylacrylamide)/carboxymethylchitosan hydrogel for drug controlled release.
27474565	1	16	theme	glutaraldehyde	387:400	arg1	crosslinking					402:413	glutaraldehyde crosslinking	387:413	glutaraldehyde crosslinking	387:413	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	1	17	dep	in	193:194	arg1	situ					196:199	situ	196:199	situ	196:199	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	0	18	theme	Multi-responsive	0:15	arg1	microsphere					26:36	Multi-responsive magnetic microsphere	0:36	Multi-responsive magnetic microsphere of poly(N-isopropylacrylamide)	0:67	Multi-responsive magnetic microsphere of poly(N-isopropylacrylamide)/carboxymethylchitosan hydrogel for drug controlled release.
27474565	3	19	theme	applied	610:616	arg1	field					627:631	an applied magnetic field	607:631	an applied magnetic field	607:631	They responded well to an applied magnetic field and showed water swelling responses to the change in solution pH and temperature.
27474565	4	20	theme	solution	842:849	arg1	LCST					864:867	LCST	864:867	LCST	864:867	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	4	20	theme	solution	842:849	arg1	50°C					871:874	50°C	871:874	50°C	871:874	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	4	20	theme	solution	842:849	arg1	temperature					851:861	its lower critical solution temperature	823:861	its lower critical solution temperature (LCST) (50°C)	823:875	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	5	21	theme	responsive	940:949	arg1	properties					951:960	These responsive properties	934:960	These responsive properties	934:960	These responsive properties can be used as triggering mechanisms for releases of the entrapped drugs from the microspheres, indicating their great potentials for use in controlled release applications.
27474565	2	22	theme	microspheres	456:467	arg1	conditions					428:437	Formulation conditions	416:437	Formulation conditions of the composite microspheres	416:467	Formulation conditions of the composite microspheres were tuned such that spherical microspheres with narrow size distributions were obtained (30.0±1.0μm in diameter).
27474565	4	23	theme	critical	833:840	arg1	LCST					864:867	LCST	864:867	LCST	864:867	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	4	23	theme	critical	833:840	arg1	50°C					871:874	50°C	871:874	50°C	871:874	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	4	23	theme	critical	833:840	arg1	temperature					851:861	its lower critical solution temperature	823:861	its lower critical solution temperature (LCST) (50°C)	823:875	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	1	24	theme	Multi-responsive	129:144	arg1	microspheres					156:167	Multi-responsive composite microspheres	129:167	Multi-responsive composite microspheres	129:167	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	1	25	theme	poly	250:253	arg1	polymerization					214:227	an in situ free radical polymerization	190:227	an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm))	190:291	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	2	26	theme	narrow	518:523	arg1	distributions					530:542	narrow size distributions	518:542	narrow size distributions	518:542	Formulation conditions of the composite microspheres were tuned such that spherical microspheres with narrow size distributions were obtained (30.0±1.0μm in diameter).
27474565	1	27	theme	carboxymethylchitosan	312:332	arg1	presence					300:307	the presence	296:307	the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs)	296:373	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	3	28	theme	solution	686:693	arg1	pH					695:696	solution pH	686:696	solution pH	686:696	They responded well to an applied magnetic field and showed water swelling responses to the change in solution pH and temperature.
27474565	5	29	used	used	969:972	arg2	properties					951:960	These responsive properties	934:960	These responsive properties	934:960	These responsive properties can be used as triggering mechanisms for releases of the entrapped drugs from the microspheres, indicating their great potentials for use in controlled release applications.
27474565	0	30	theme	poly	41:44	arg1	microsphere					26:36	Multi-responsive magnetic microsphere	0:36	Multi-responsive magnetic microsphere of poly(N-isopropylacrylamide)	0:67	Multi-responsive magnetic microsphere of poly(N-isopropylacrylamide)/carboxymethylchitosan hydrogel for drug controlled release.
27474565	4	31	theme	solution	792:799	arg1	temperature					801:811	the solution temperature	788:811	the solution temperature	788:811	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	3	32	theme	magnetic	618:625	arg1	field					627:631	an applied magnetic field	607:631	an applied magnetic field	607:631	They responded well to an applied magnetic field and showed water swelling responses to the change in solution pH and temperature.
27474565	1	33	theme	composite	146:154	arg1	microspheres					156:167	Multi-responsive composite microspheres	129:167	Multi-responsive composite microspheres	129:167	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	2	34	theme	spherical	490:498	arg1	microspheres					500:511	spherical microspheres	490:511	spherical microspheres with narrow size distributions	490:542	Formulation conditions of the composite microspheres were tuned such that spherical microspheres with narrow size distributions were obtained (30.0±1.0μm in diameter).
27474565	5	35	theme	release	1114:1120	arg1	applications					1122:1133	controlled release applications	1103:1133	controlled release applications	1103:1133	These responsive properties can be used as triggering mechanisms for releases of the entrapped drugs from the microspheres, indicating their great potentials for use in controlled release applications.
27474565	2	36	from	30.0±1.0μm	559:568	arg1	diameter					573:580	diameter	573:580	diameter	573:580	Formulation conditions of the composite microspheres were tuned such that spherical microspheres with narrow size distributions were obtained (30.0±1.0μm in diameter).
27474565	4	37	theme	entrapped	733:741	arg1	drug					762:765	an entrapped indomethacin model drug	730:765	an entrapped indomethacin model drug	730:765	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	3	38	theme	swelling	650:657	arg1	responses					659:667	water swelling responses	644:667	water swelling responses to the change in solution pH and temperature	644:712	They responded well to an applied magnetic field and showed water swelling responses to the change in solution pH and temperature.
27474565	2	39	dep	obtained	549:556	arg1	such					480:483	such	480:483	such	480:483	Formulation conditions of the composite microspheres were tuned such that spherical microspheres with narrow size distributions were obtained (30.0±1.0μm in diameter).
27474565	2	39	dep	obtained	549:556	arg1	30.0±1.0μm					559:568	30.0±1.0μm	559:568	30.0±1.0μm in diameter	559:580	Formulation conditions of the composite microspheres were tuned such that spherical microspheres with narrow size distributions were obtained (30.0±1.0μm in diameter).
27474565	4	40	theme	lower	827:831	arg1	LCST					864:867	LCST	864:867	LCST	864:867	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	4	40	theme	lower	827:831	arg1	50°C					871:874	50°C	871:874	50°C	871:874	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	4	40	theme	lower	827:831	arg1	temperature					851:861	its lower critical solution temperature	823:861	its lower critical solution temperature (LCST) (50°C)	823:875	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	4	41	theme	drug	762:765	arg1	release					719:725	The release	715:725	The release of an entrapped indomethacin model drug	715:765	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	4	42	theme	basic	908:912	arg1	pH					926:927	pH 11	926:930	pH 11	926:930	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	4	42	theme	basic	908:912	arg1	conditions					914:923	basic conditions	908:923	basic conditions (pH 11)	908:931	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	2	43	with	microspheres	500:511	arg1	distributions					530:542	narrow size distributions	518:542	narrow size distributions	518:542	Formulation conditions of the composite microspheres were tuned such that spherical microspheres with narrow size distributions were obtained (30.0±1.0μm in diameter).
27474565	4	44	theme	model	756:760	arg1	drug					762:765	an entrapped indomethacin model drug	730:765	an entrapped indomethacin model drug	730:765	The release of an entrapped indomethacin model drug was accelerated when the solution temperature was above its lower critical solution temperature (LCST) (50°C) or when the solution pH was in basic conditions (pH 11).
27474565	3	45	theme	water	644:648	arg1	responses					659:667	water swelling responses	644:667	water swelling responses to the change in solution pH and temperature	644:712	They responded well to an applied magnetic field and showed water swelling responses to the change in solution pH and temperature.
27474565	1	46	theme	thermo-responsive	232:248	arg1	poly					250:253	thermo-responsive poly	232:253	thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm))	232:291	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	1	46	theme	thermo-responsive	232:248	arg1	N-isopropylacrylamide					255:275	N-isopropylacrylamide	255:275	N-isopropylacrylamide	255:275	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	2	47	theme	Formulation	416:426	arg1	conditions					428:437	Formulation conditions	416:437	Formulation conditions of the composite microspheres	416:467	Formulation conditions of the composite microspheres were tuned such that spherical microspheres with narrow size distributions were obtained (30.0±1.0μm in diameter).
27474565	1	48	theme	magnetite	344:352	arg1	MNPs					369:372	MNPs	369:372	MNPs	369:372	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	1	48	theme	magnetite	344:352	arg1	nanoparticles					354:366	magnetite nanoparticles	344:366	magnetite nanoparticles (MNPs)	344:373	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	1	49	dep	poly	250:253	arg1	poly					279:282	poly	279:282	poly(NIPAAm)	279:290	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	1	49	dep	poly	250:253	arg1	NIPAAm					284:289	NIPAAm	284:289	NIPAAm	284:289	Multi-responsive composite microspheres were synthesized via an in situ free radical polymerization of thermo-responsive poly(N-isopropylacrylamide) (poly(NIPAAm)) in the presence of carboxymethylchitosan (CMC) and magnetite nanoparticles (MNPs) followed by glutaraldehyde crosslinking.
27474565	3	50	from	change	676:681	arg1	temperature					702:712	temperature	702:712	temperature	702:712	They responded well to an applied magnetic field and showed water swelling responses to the change in solution pH and temperature.
27474565	3	50	from	change	676:681	arg1	pH					695:696	solution pH	686:696	solution pH	686:696	They responded well to an applied magnetic field and showed water swelling responses to the change in solution pH and temperature.
27308199	0	0	theme	Use	93:95	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans	0:138	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	1	1	theme	synthesis	369:377	arg1	properties					397:406	the synthesis and glycosylation properties	365:406	properties	397:406	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	1	theme	synthesis	369:377	arg1	part					178:181	part	178:181	part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans	178:362	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	3	2	theme	approach	1177:1184	arg1	viability					1145:1153	the viability	1141:1153	the viability of the building block approach	1141:1184	The glycosylation reactions proceeded with high yields and α-selectivity, proving the viability of the building block approach also for construction of 4-O-xylosyl-containing C. neoformans CPS structures.
27308199	2	3	theme	2-naphthalenylmethyl	735:754	arg1	-1-thio-α-d-mannopyranoside					756:782	-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	673:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	606:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	1	4	theme	glycosylation	383:395	arg1	properties					397:406	the synthesis and glycosylation properties	365:406	properties	397:406	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	4	theme	glycosylation	383:395	arg1	part					178:181	part	178:181	part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans	178:362	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	3	5	theme	building	1162:1169	arg1	block					1171:1175	the building block	1158:1175	the building block approach	1158:1184	The glycosylation reactions proceeded with high yields and α-selectivity, proving the viability of the building block approach also for construction of 4-O-xylosyl-containing C. neoformans CPS structures.
27308199	1	6	theme	common	492:497	arg1	block					508:512	a common building block	490:512	a common building block for construction of serotype B and C capsular polysaccharide structures	490:584	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	6	theme	common	492:497	arg1	thioglycoside					475:487	a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside	411:487	a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside	411:487	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	2	7	theme	suitable	928:935	arg1	hexa-					891:895	hexa-	891:895	hexa-	891:895	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	1	8	dep	protected	451:459	arg1	NAP					433:435	NAP	433:435	NAP	433:435	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	8	dep	protected	451:459	arg1	naphthalenylmethyl					413:430	naphthalenylmethyl	413:430	naphthalenylmethyl	413:430	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	3	9	theme	high	1102:1105	arg1	yields					1107:1112	high yields	1102:1112	high yields	1102:1112	The glycosylation reactions proceeded with high yields and α-selectivity, proving the viability of the building block approach also for construction of 4-O-xylosyl-containing C. neoformans CPS structures.
27308199	3	10	theme	block	1171:1175	arg1	approach					1177:1184	the building block approach	1158:1184	the building block approach	1158:1184	The glycosylation reactions proceeded with high yields and α-selectivity, proving the viability of the building block approach also for construction of 4-O-xylosyl-containing C. neoformans CPS structures.
27308199	1	11	theme	building	499:506	arg1	block					508:512	a common building block	490:512	a common building block for construction of serotype B and C capsular polysaccharide structures	490:584	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	11	theme	building	499:506	arg1	thioglycoside					475:487	a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside	411:487	a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside	411:487	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	2	12	dep	donor	816:820	arg1	both					806:809	both	806:809	both	806:809	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	2	12	dep	donor	816:820	arg1	a					814:814	a	814:814	a	814:814	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	1	13	theme	vaccine	293:299	arg1	candidates					301:310	vaccine candidates	293:310	vaccine candidates	293:310	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	2	14	theme	glycosylation	841:853	arg1	reactions					855:863	glycosylation reactions	841:863	glycosylation reactions	841:863	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	2	15	theme	-6-O-benzyl-3-O-	718:733	arg1	-1-thio-α-d-mannopyranoside					756:782	-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	673:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	606:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	2	16	dep	Ethyl	606:610	arg1	-1-thio-α-d-mannopyranoside					756:782	-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	673:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	606:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	2	17	theme	1→4	713:715	arg1	-1-thio-α-d-mannopyranoside					756:782	-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	673:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	606:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	2	18	theme	glycan	1009:1014	arg1	array					1016:1020	a glycan array	1007:1020	a glycan array	1007:1020	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	1	19	theme	ongoing	189:195	arg1	project					197:203	an ongoing project	186:203	an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans	186:362	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	3	20	theme	C. neoformans	1234:1246	arg1	structures					1252:1261	4-O-xylosyl-containing C. neoformans CPS structures	1211:1261	4-O-xylosyl-containing C. neoformans CPS structures	1211:1261	The glycosylation reactions proceeded with high yields and α-selectivity, proving the viability of the building block approach also for construction of 4-O-xylosyl-containing C. neoformans CPS structures.
27308199	1	21	theme	candidates	301:310	arg1	development					278:288	the development	274:288	the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans	274:362	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	2	22	theme	-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-	673:711	arg1	-1-thio-α-d-mannopyranoside					756:782	-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	673:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	606:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	1	23	theme	project	197:203	arg1	properties					397:406	the synthesis and glycosylation properties	365:406	properties	397:406	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	23	theme	project	197:203	arg1	part					178:181	part	178:181	part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans	178:362	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	0	24	theme	Glucuronic	15:24	arg1	Block					79:83	a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block	13:83	a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block	13:83	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	0	25	theme	Neoformans	129:138	arg1	Assembly					104:111	the Assembly	100:111	the Assembly of Cryptococcus Neoformans	100:138	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	1	26	theme	serotype	534:541	arg1	B					543:543	serotype B	534:543	serotype B	534:543	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	2	27	from	donor	816:820	arg1	reactions					855:863	glycosylation reactions	841:863	glycosylation reactions	841:863	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	0	28	theme	Cryptococcus	116:127	arg1	Neoformans					129:138	Cryptococcus Neoformans	116:138	Cryptococcus Neoformans	116:138	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	0	29	from	Use	93:95	arg1	Assembly					104:111	the Assembly	100:111	the Assembly of Cryptococcus Neoformans	100:138	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	2	30	used	used	801:804	arg2	acceptor					829:836	an acceptor	826:836	an acceptor in glycosylation reactions	826:863	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	2	30	used	used	801:804	arg2	donor					816:820	donor	816:820	donor	816:820	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	2	30	used	used	801:804	arg2	uronate					659:665	benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate	613:665	benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate	613:665	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	2	30	used	used	801:804	arg2	Ethyl					606:610	Ethyl	606:610	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	606:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	0	31	theme	Thioglycoside	42:54	arg1	Block					79:83	a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block	13:83	a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block	13:83	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	1	32	theme	fungal	324:329	arg1	neoformans					353:362	the fungal pathogen Cryptococcus neoformans	320:362	the fungal pathogen Cryptococcus neoformans	320:362	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	0	33	theme	Polysaccharide	149:162	arg1	Fragments					164:172	Capsular Polysaccharide Fragments	140:172	Capsular Polysaccharide Fragments	140:172	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	3	34	theme	CPS	1248:1250	arg1	structures					1252:1261	4-O-xylosyl-containing C. neoformans CPS structures	1211:1261	4-O-xylosyl-containing C. neoformans CPS structures	1211:1261	The glycosylation reactions proceeded with high yields and α-selectivity, proving the viability of the building block approach also for construction of 4-O-xylosyl-containing C. neoformans CPS structures.
27308199	0	35	theme	Acid-Containing	26:40	arg1	Block					79:83	a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block	13:83	a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block	13:83	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	1	36	theme	pathogen	331:338	arg1	neoformans					353:362	the fungal pathogen Cryptococcus neoformans	320:362	the fungal pathogen Cryptococcus neoformans	320:362	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	0	37	theme	Capsular	140:147	arg1	Fragments					164:172	Capsular Polysaccharide Fragments	140:172	Capsular Polysaccharide Fragments	140:172	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	2	38	from	acceptor	829:836	arg1	reactions					855:863	glycosylation reactions	841:863	glycosylation reactions	841:863	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	1	39	theme	B	543:543	arg1	structures					575:584	serotype B and C capsular polysaccharide structures	534:584	serotype B and C capsular polysaccharide structures	534:584	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	40	theme	protective	226:235	arg1	epitopes					261:268	protective capsular polysaccharide epitopes	226:268	protective capsular polysaccharide epitopes	226:268	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	0	41	theme	Building	70:77	arg1	Block					79:83	a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block	13:83	a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block	13:83	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	1	42	theme	Cryptococcus	340:351	arg1	neoformans					353:362	the fungal pathogen Cryptococcus neoformans	320:362	the fungal pathogen Cryptococcus neoformans	320:362	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	0	43	from	Block	79:83	arg1	Assembly					104:111	the Assembly	100:111	the Assembly of Cryptococcus Neoformans	100:138	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	3	44	theme	4-O-xylosyl-containing	1211:1232	arg1	structures					1252:1261	4-O-xylosyl-containing C. neoformans CPS structures	1211:1261	4-O-xylosyl-containing C. neoformans CPS structures	1211:1261	The glycosylation reactions proceeded with high yields and α-selectivity, proving the viability of the building block approach also for construction of 4-O-xylosyl-containing C. neoformans CPS structures.
27308199	0	45	theme	Trisaccharide	56:68	arg1	Block					79:83	a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block	13:83	a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block	13:83	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	3	46	theme	structures	1252:1261	arg1	construction					1195:1206	construction	1195:1206	construction of 4-O-xylosyl-containing C. neoformans CPS structures	1195:1261	The glycosylation reactions proceeded with high yields and α-selectivity, proving the viability of the building block approach also for construction of 4-O-xylosyl-containing C. neoformans CPS structures.
27308199	1	47	theme	protected	451:459	arg1	block					508:512	a common building block	490:512	a common building block for construction of serotype B and C capsular polysaccharide structures	490:584	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	47	theme	protected	451:459	arg1	thioglycoside					475:487	a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside	411:487	a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside	411:487	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	48	theme	trisaccharide	461:473	arg1	block					508:512	a common building block	490:512	a common building block for construction of serotype B and C capsular polysaccharide structures	490:584	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	48	theme	trisaccharide	461:473	arg1	thioglycoside					475:487	a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside	411:487	a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside	411:487	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	49	theme	C	549:549	arg1	polysaccharide					560:573	C capsular polysaccharide	549:573	C capsular polysaccharide	549:573	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	2	50	theme	2,3,4-tri-O-benzyl-β-d-glucopyranosyl-	620:657	arg1	Ethyl					606:610	Ethyl	606:610	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	606:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	2	50	theme	2,3,4-tri-O-benzyl-β-d-glucopyranosyl-	620:657	arg1	uronate					659:665	benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate	613:665	benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate	613:665	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	0	51	from	Assembly	104:111	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans	0:138	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	1	52	theme	capsular	551:558	arg1	polysaccharide					560:573	C capsular polysaccharide	549:573	C capsular polysaccharide	549:573	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	2	53	theme	heptasaccharide	901:915	arg1	structures					917:926	heptasaccharide structures	901:926	heptasaccharide structures	901:926	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	1	54	theme	capsular	237:244	arg1	epitopes					261:268	protective capsular polysaccharide epitopes	226:268	protective capsular polysaccharide epitopes	226:268	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	2	55	theme	benzyl	613:618	arg1	Ethyl					606:610	Ethyl	606:610	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside	606:782	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	2	55	theme	benzyl	613:618	arg1	uronate					659:665	benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate	613:665	benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate	613:665	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	1	56	theme	thioglycoside	475:487	arg1	properties					397:406	the synthesis and glycosylation properties	365:406	properties	397:406	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	56	theme	thioglycoside	475:487	arg1	part					178:181	part	178:181	part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans	178:362	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	3	57	theme	glycosylation	1063:1075	arg1	reactions					1077:1085	The glycosylation reactions	1059:1085	The glycosylation reactions	1059:1085	The glycosylation reactions proceeded with high yields and α-selectivity, proving the viability of the building block approach also for construction of 4-O-xylosyl-containing C. neoformans CPS structures.
27308199	1	58	theme	polysaccharide	560:573	arg1	structures					575:584	serotype B and C capsular polysaccharide structures	534:584	serotype B and C capsular polysaccharide structures	534:584	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	0	59	dep	Fragments	164:172	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans	0:138	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	1	60	theme	polysaccharide	246:259	arg1	epitopes					261:268	protective capsular polysaccharide epitopes	226:268	protective capsular polysaccharide epitopes	226:268	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	1	61	theme	structures	575:584	arg1	construction					518:529	construction	518:529	construction of serotype B and C capsular polysaccharide structures	518:584	As part of an ongoing project aimed at identifying protective capsular polysaccharide epitopes for the development of vaccine candidates against the fungal pathogen Cryptococcus neoformans, the synthesis and glycosylation properties of a naphthalenylmethyl (NAP) orthogonally protected trisaccharide thioglycoside, a common building block for construction of serotype B and C capsular polysaccharide structures, were investigated.
27308199	0	62	theme	Block	79:83	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans	0:138	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	0	63	from	Synthesis	0:8	arg1	Assembly					104:111	the Assembly	100:111	the Assembly of Cryptococcus Neoformans	100:138	Synthesis of a Glucuronic Acid-Containing Thioglycoside Trisaccharide Building Block and Its Use in the Assembly of Cryptococcus Neoformans Capsular Polysaccharide Fragments.
27308199	2	64	theme	continued	948:956	arg1	elongation					958:967	continued elongation	948:967	continued elongation	948:967	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
27308199	2	65	theme	carrier	1042:1048	arg1	protein					1050:1056	a carrier protein	1040:1056	a carrier protein	1040:1056	Ethyl (benzyl 2,3,4-tri-O-benzyl-β-d-glucopyranosyl- uronate)-(1→2)-[2,3,4-tri-O-benzyl-β-d-xylopyranosyl-(1→4)]-6-O-benzyl-3-O-(2-naphthalenylmethyl)-1-thio-α-d-mannopyranoside was prepared and used both as a donor and an acceptor in glycosylation reactions to obtain spacer equipped hexa- and heptasaccharide structures suitable either for continued elongation or for deprotection and printing onto a glycan array or conjugation to a carrier protein.
28821155	2	0	theme	different	465:473	arg1	concentrations					475:488	different concentrations	465:488	(ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure	460:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	5	1	with	degree	984:989	arg1	structure					1008:1016	predominant structure	996:1016	predominant structure of cellulose Iβ	996:1032	The increasing concentration of xyloglucan to pectin also caused the drop down in microfibrils crystallinity degree with predominant structure of cellulose Iβ.
28821155	2	2	theme	simultaneous	347:358	arg1	influence					360:368	the simultaneous influence	343:368	the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure	343:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	1	3	theme	walls	251:255	arg1	properties					232:241	the mechanical properties	217:241	the mechanical properties of cell walls	217:255	The impact of the matrix polysaccharides on the cellulose microfibrils structure as well as on the mechanical properties of cell walls still remains an open question.
28821155	0	4	from	influence	13:21	arg1	properties					76:85	mechanical properties	65:85	mechanical properties	65:85	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites.
28821155	0	4	from	influence	13:21	arg1	structure					51:59	structure	51:59	structure	51:59	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites.
28821155	4	5	theme	cellulose	838:846	arg1	microfibrils					848:859	cellulose microfibrils	838:859	cellulose microfibrils were thinner	838:872	This investigation showed that the lower the ratio of xyloglucan to pectin was, the higher Young's modulus of BC composite was and also obtained cellulose microfibrils were thinner.
28821155	6	6	theme	cellulose	1068:1076	arg1	chains					1078:1083	cellulose chains	1068:1083	cellulose chains	1068:1083	In that case, also the length of cellulose chains was growing and reaching the highest value among all BC composites.
28821155	2	7	from	structure	556:564	arg1	concentration					518:530	constant concentration	509:530	constant concentration of pectins on cellulose structure	509:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	0	8	theme	cellulose	100:108	arg1	composites					110:119	bacterial cellulose composites	90:119	bacterial cellulose composites	90:119	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites.
28821155	3	9	theme	bacterial	581:589	arg1	BC					602:603	BC	602:603	BC	602:603	Composites of bacterial cellulose (BC) produced by Komagataeibacter xylinus are considered to mimic natural plant cell walls.
28821155	3	9	theme	bacterial	581:589	arg1	cellulose					591:599	bacterial cellulose	581:599	bacterial cellulose (BC) produced by Komagataeibacter xylinus	581:641	Composites of bacterial cellulose (BC) produced by Komagataeibacter xylinus are considered to mimic natural plant cell walls.
28821155	0	10	theme	bacterial	90:98	arg1	composites					110:119	bacterial cellulose composites	90:119	bacterial cellulose composites	90:119	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites.
28821155	5	11	theme	increasing	879:888	arg1	concentration					890:902	The increasing concentration	875:902	The increasing concentration of xyloglucan to pectin	875:926	The increasing concentration of xyloglucan to pectin also caused the drop down in microfibrils crystallinity degree with predominant structure of cellulose Iβ.
28821155	6	12	theme	chains	1078:1083	arg1	length					1058:1063	also the length	1049:1063	also the length of cellulose chains	1049:1083	In that case, also the length of cellulose chains was growing and reaching the highest value among all BC composites.
28821155	5	13	theme	microfibrils	957:968	arg1	degree					984:989	microfibrils crystallinity degree	957:989	microfibrils crystallinity degree with predominant structure of cellulose Iβ	957:1032	The increasing concentration of xyloglucan to pectin also caused the drop down in microfibrils crystallinity degree with predominant structure of cellulose Iβ.
28821155	1	14	theme	cellulose	170:178	arg1	microfibrils					180:191	the cellulose microfibrils	166:191	the cellulose microfibrils structure	166:201	The impact of the matrix polysaccharides on the cellulose microfibrils structure as well as on the mechanical properties of cell walls still remains an open question.
28821155	0	15	theme	composites	110:119	arg1	properties					76:85	mechanical properties	65:85	mechanical properties	65:85	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites.
28821155	0	15	theme	composites	110:119	arg1	structure					51:59	structure	51:59	structure	51:59	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites.
28821155	5	16	theme	crystallinity	970:982	arg1	degree					984:989	microfibrils crystallinity degree	957:989	microfibrils crystallinity degree with predominant structure of cellulose Iβ	957:1032	The increasing concentration of xyloglucan to pectin also caused the drop down in microfibrils crystallinity degree with predominant structure of cellulose Iβ.
28821155	1	17	theme	microfibrils	180:191	arg1	structure					193:201	the cellulose microfibrils structure	166:201	the cellulose microfibrils structure	166:201	The impact of the matrix polysaccharides on the cellulose microfibrils structure as well as on the mechanical properties of cell walls still remains an open question.
28821155	2	18	theme	xyloglucan	444:453	arg1	concentration					427:439	constant concentration	418:439	constant concentration of xyloglucan	418:453	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	2	19	with	pectins	405:411	arg1	concentration					427:439	constant concentration	418:439	constant concentration of xyloglucan	418:453	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	2	20	with	xyloglucan	493:502	arg1	concentration					518:530	constant concentration	509:530	constant concentration of pectins on cellulose structure	509:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	4	21	dep	lower	728:732	arg1	ratio					738:742	the ratio	734:742	the lower the ratio of xyloglucan to pectin	724:766	This investigation showed that the lower the ratio of xyloglucan to pectin was, the higher Young's modulus of BC composite was and also obtained cellulose microfibrils were thinner.
28821155	0	22	theme	Simultaneous	0:11	arg1	influence					13:21	Simultaneous influence	0:21	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites	0:119	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites.
28821155	1	23	theme	open	274:277	arg1	question					279:286	an open question	271:286	an open question	271:286	The impact of the matrix polysaccharides on the cellulose microfibrils structure as well as on the mechanical properties of cell walls still remains an open question.
28821155	3	24	theme	natural	667:673	arg1	walls					686:690	natural plant cell walls	667:690	natural plant cell walls	667:690	Composites of bacterial cellulose (BC) produced by Komagataeibacter xylinus are considered to mimic natural plant cell walls.
28821155	0	25	theme	pectin	26:31	arg1	influence					13:21	Simultaneous influence	0:21	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites	0:119	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites.
28821155	2	26	theme	cellulose	546:554	arg1	structure					556:564	cellulose structure	546:564	cellulose structure	546:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	1	27	from	impact	126:131	arg1	structure					193:201	the cellulose microfibrils structure	166:201	the cellulose microfibrils structure	166:201	The impact of the matrix polysaccharides on the cellulose microfibrils structure as well as on the mechanical properties of cell walls still remains an open question.
28821155	1	27	from	impact	126:131	arg1	properties					232:241	the mechanical properties	217:241	the mechanical properties of cell walls	217:255	The impact of the matrix polysaccharides on the cellulose microfibrils structure as well as on the mechanical properties of cell walls still remains an open question.
28821155	4	28	theme	composite	806:814	arg1	modulus					792:798	the higher Young's modulus	773:798	the higher Young's modulus of BC composite	773:814	This investigation showed that the lower the ratio of xyloglucan to pectin was, the higher Young's modulus of BC composite was and also obtained cellulose microfibrils were thinner.
28821155	2	29	theme	constant	418:425	arg1	concentration					427:439	constant concentration	418:439	constant concentration of xyloglucan	418:453	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	5	30	theme	Iβ	1031:1032	arg1	structure					1008:1016	predominant structure	996:1016	predominant structure of cellulose Iβ	996:1032	The increasing concentration of xyloglucan to pectin also caused the drop down in microfibrils crystallinity degree with predominant structure of cellulose Iβ.
28821155	2	31	theme	pectins	405:411	arg1	concentrations					475:488	different concentrations	465:488	(ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure	460:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	2	31	theme	pectins	405:411	arg1	concentrations					387:400	different concentrations	377:400	(i) different concentrations of pectins with constant concentration of xyloglucan	373:453	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	0	32	theme	xyloglucan	37:46	arg1	influence					13:21	Simultaneous influence	0:21	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites	0:119	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites.
28821155	5	33	theme	xyloglucan	907:916	arg1	concentration					890:902	The increasing concentration	875:902	The increasing concentration of xyloglucan to pectin	875:926	The increasing concentration of xyloglucan to pectin also caused the drop down in microfibrils crystallinity degree with predominant structure of cellulose Iβ.
28821155	2	34	theme	pectins	535:541	arg1	concentration					518:530	constant concentration	509:530	constant concentration of pectins on cellulose structure	509:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	6	35	theme	BC	1138:1139	arg1	composites					1141:1150	all BC composites	1134:1150	all BC composites	1134:1150	In that case, also the length of cellulose chains was growing and reaching the highest value among all BC composites.
28821155	2	36	theme	different	377:385	arg1	concentrations					387:400	different concentrations	377:400	(i) different concentrations of pectins with constant concentration of xyloglucan	373:453	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	2	37	dep	concentrations	387:400	arg1	i					374:374	i	374:374	i	374:374	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	2	38	theme	xyloglucan	493:502	arg1	concentrations					475:488	different concentrations	465:488	(ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure	460:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	2	38	theme	xyloglucan	493:502	arg1	concentrations					387:400	different concentrations	377:400	(i) different concentrations of pectins with constant concentration of xyloglucan	373:453	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	4	39	theme	BC	803:804	arg1	composite					806:814	BC composite	803:814	BC composite	803:814	This investigation showed that the lower the ratio of xyloglucan to pectin was, the higher Young's modulus of BC composite was and also obtained cellulose microfibrils were thinner.
28821155	2	40	theme	constant	509:516	arg1	concentration					518:530	constant concentration	509:530	constant concentration of pectins on cellulose structure	509:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	6	41	theme	highest	1114:1120	arg1	value					1122:1126	the highest value	1110:1126	the highest value among all BC composites	1110:1150	In that case, also the length of cellulose chains was growing and reaching the highest value among all BC composites.
28821155	3	42	theme	plant	675:679	arg1	walls					686:690	natural plant cell walls	667:690	natural plant cell walls	667:690	Composites of bacterial cellulose (BC) produced by Komagataeibacter xylinus are considered to mimic natural plant cell walls.
28821155	3	43	theme	cellulose	591:599	arg1	Composites					567:576	Composites	567:576	Composites of bacterial cellulose (BC) produced by Komagataeibacter xylinus	567:641	Composites of bacterial cellulose (BC) produced by Komagataeibacter xylinus are considered to mimic natural plant cell walls.
28821155	3	43	theme	cellulose	591:599	arg1	cellulose					591:599	bacterial cellulose	581:599	bacterial cellulose (BC) produced by Komagataeibacter xylinus	581:641	Composites of bacterial cellulose (BC) produced by Komagataeibacter xylinus are considered to mimic natural plant cell walls.
28821155	2	44	from	pectins	535:541	arg1	structure					556:564	cellulose structure	546:564	cellulose structure	546:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	3	45	theme	cell	681:684	arg1	walls					686:690	natural plant cell walls	667:690	natural plant cell walls	667:690	Composites of bacterial cellulose (BC) produced by Komagataeibacter xylinus are considered to mimic natural plant cell walls.
28821155	4	46	theme	higher	777:782	arg1	modulus					792:798	the higher Young's modulus	773:798	the higher Young's modulus of BC composite	773:814	This investigation showed that the lower the ratio of xyloglucan to pectin was, the higher Young's modulus of BC composite was and also obtained cellulose microfibrils were thinner.
28821155	5	47	theme	cellulose	1021:1029	arg1	Iβ					1031:1032	cellulose Iβ	1021:1032	cellulose Iβ	1021:1032	The increasing concentration of xyloglucan to pectin also caused the drop down in microfibrils crystallinity degree with predominant structure of cellulose Iβ.
28821155	1	48	theme	mechanical	221:230	arg1	properties					232:241	the mechanical properties	217:241	the mechanical properties of cell walls	217:255	The impact of the matrix polysaccharides on the cellulose microfibrils structure as well as on the mechanical properties of cell walls still remains an open question.
28821155	2	49	dep	concentrations	475:488	arg1	ii					461:462	ii	461:462	ii	461:462	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	2	50	from	concentration	518:530	arg1	structure					556:564	cellulose structure	546:564	cellulose structure	546:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	3	51	theme	Komagataeibacter	618:633	arg1	xylinus					635:641	Komagataeibacter xylinus	618:641	Komagataeibacter xylinus	618:641	Composites of bacterial cellulose (BC) produced by Komagataeibacter xylinus are considered to mimic natural plant cell walls.
28821155	2	52	theme	investigations	311:324	arg1	aim					304:306	the aim	300:306	the aim of investigations	300:324	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	0	53	theme	mechanical	65:74	arg1	properties					76:85	mechanical properties	65:85	mechanical properties	65:85	Simultaneous influence of pectin and xyloglucan on structure and mechanical properties of bacterial cellulose composites.
28821155	2	54	theme	concentrations	387:400	arg1	influence					360:368	the simultaneous influence	343:368	the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure	343:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	5	55	theme	predominant	996:1006	arg1	structure					1008:1016	predominant structure	996:1016	predominant structure of cellulose Iβ	996:1032	The increasing concentration of xyloglucan to pectin also caused the drop down in microfibrils crystallinity degree with predominant structure of cellulose Iβ.
28821155	1	56	theme	matrix	140:145	arg1	polysaccharides					147:161	the matrix polysaccharides	136:161	the matrix polysaccharides	136:161	The impact of the matrix polysaccharides on the cellulose microfibrils structure as well as on the mechanical properties of cell walls still remains an open question.
28821155	2	57	theme	concentrations	475:488	arg1	influence					360:368	the simultaneous influence	343:368	the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure	343:564	Therefore, the aim of investigations was to determine the simultaneous influence of (i) different concentrations of pectins with constant concentration of xyloglucan, and (ii) different concentrations of xyloglucan with constant concentration of pectins on cellulose structure.
28821155	4	58	theme	Young	784:788	arg1	modulus					792:798	the higher Young's modulus	773:798	the higher Young's modulus of BC composite	773:814	This investigation showed that the lower the ratio of xyloglucan to pectin was, the higher Young's modulus of BC composite was and also obtained cellulose microfibrils were thinner.
28821155	1	59	theme	polysaccharides	147:161	arg1	impact					126:131	The impact	122:131	The impact of the matrix polysaccharides on the cellulose microfibrils structure as well as on the mechanical properties of cell walls	122:255	The impact of the matrix polysaccharides on the cellulose microfibrils structure as well as on the mechanical properties of cell walls still remains an open question.
28821155	1	60	theme	cell	246:249	arg1	walls					251:255	cell walls	246:255	cell walls	246:255	The impact of the matrix polysaccharides on the cellulose microfibrils structure as well as on the mechanical properties of cell walls still remains an open question.
28944737	1	0	theme	able	161:164	arg1	AIM					120:122	AIM	120:122	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR)	120:235	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
28944737	4	1	theme	comparable	594:603	arg1	amount					605:610	comparable amount	594:610	comparable amount (i.e., 60/40 and 50/50)	594:634	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	4	2	from	RYR	684:686	arg1	release					657:663	a delayed release	647:663	a delayed release of lovastatin from RYR	647:686	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	4	2	from	RYR	684:686	arg1	activity					712:719	a prolonged inhibitory activity	689:719	a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase	689:774	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	4	2	from	RYR	684:686	arg1	synthesis					804:812	a decreased cholesterol synthesis	780:812	a decreased cholesterol synthesis	780:812	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	5	3	theme	compatible	973:982	arg1	profile					965:971	a lovastatin release profile	944:971	a lovastatin release profile compatible with cholesterol biosynthesis	944:1012	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	4	4	theme	decreased	782:790	arg1	synthesis					804:812	a decreased cholesterol synthesis	780:812	a decreased cholesterol synthesis	780:812	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	0	5	theme	hypercholesterolemia	90:109	arg1	therapy					111:117	hypercholesterolemia therapy	90:117	hypercholesterolemia therapy	90:117	Alginate-gelatin formulation to modify lovastatin release profile from red yeast rice for hypercholesterolemia therapy.
28944737	4	6	theme	inhibitory	701:710	arg1	activity					712:719	a prolonged inhibitory activity	689:719	a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase	689:774	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	5	7	theme	alginate	884:891	arg1	alginate					884:891	alginate	884:891	alginate	884:891	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	5	7	theme	alginate	884:891	arg1	gelatin					865:871	60% gelatin	861:871	60% gelatin	861:871	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	5	7	theme	alginate	884:891	arg1	%					879:879	40%	877:879	40% of alginate	877:891	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	4	8	theme	lovastatin	668:677	arg1	release					657:663	a delayed release	647:663	a delayed release of lovastatin from RYR	647:686	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	4	8	theme	lovastatin	668:677	arg1	activity					712:719	a prolonged inhibitory activity	689:719	a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase	689:774	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	4	8	theme	lovastatin	668:677	arg1	synthesis					804:812	a decreased cholesterol synthesis	780:812	a decreased cholesterol synthesis	780:812	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	4	9	theme	cholesterol	792:802	arg1	synthesis					804:812	a decreased cholesterol synthesis	780:812	a decreased cholesterol synthesis	780:812	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	2	10	theme	different	391:399	arg1	ratios					401:406	different ratios	391:406	different ratios	391:406	MATERIALS & METHODS Polymeric formulations were prepared mixing alginate and gelatin, in different ratios, with RYR.
28944737	3	11	theme	matrices	519:526	arg1	mesostructure					502:514	mesostructure	502:514	mesostructure	502:514	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices was analyzed.
28944737	3	11	theme	matrices	519:526	arg1	viscosity					469:477	viscosity	469:477	viscosity	469:477	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices was analyzed.
28944737	3	11	theme	matrices	519:526	arg1	behavior					489:496	swelling behavior	480:496	swelling behavior	480:496	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices was analyzed.
28944737	3	11	theme	matrices	519:526	arg1	stiffness					458:466	stiffness	458:466	stiffness	458:466	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices was analyzed.
28944737	4	12	theme	delayed	649:655	arg1	release					657:663	a delayed release	647:663	a delayed release of lovastatin from RYR	647:686	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	4	13	theme	A	764:764	arg1	reductase					766:774	3-hydroxy-3-methylglutaryl-coenzyme A reductase	728:774	3-hydroxy-3-methylglutaryl-coenzyme A reductase	728:774	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	1	14	theme	delayed	181:187	arg1	release					189:195	a delayed release	179:195	a delayed release of lovastatin from red yeast rice (RYR)	179:235	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
28944737	0	15	theme	Alginate-gelatin	0:15	arg1	formulation					17:27	Alginate-gelatin formulation	0:27	Alginate-gelatin formulation	0:27	Alginate-gelatin formulation to modify lovastatin release profile from red yeast rice for hypercholesterolemia therapy.
28944737	5	16	dep	CONCLUSION	815:824	arg1	showed					893:898	showed	893:898	showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis	893:1012	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	2	17	theme	&	312:312	arg1	METHODS					314:320	MATERIALS & METHODS Polymeric formulations	302:343	MATERIALS & METHODS Polymeric formulations	302:343	MATERIALS & METHODS Polymeric formulations were prepared mixing alginate and gelatin, in different ratios, with RYR.
28944737	4	18	dep	amount	605:610	arg1	50/50					629:633	50/50	629:633	50/50	629:633	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	4	18	dep	amount	605:610	arg1	60/40					619:623	60/40	619:623	60/40	619:623	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	5	19	theme	60	861:862	arg1	%					863:863	%	863:863	%	863:863	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	1	20	theme	cholesterol	264:274	arg1	biosynthesis					276:287	cholesterol biosynthesis	264:287	cholesterol biosynthesis	264:287	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
28944737	2	21	theme	MATERIALS	302:310	arg1	METHODS					314:320	MATERIALS & METHODS Polymeric formulations	302:343	MATERIALS & METHODS Polymeric formulations	302:343	MATERIALS & METHODS Polymeric formulations were prepared mixing alginate and gelatin, in different ratios, with RYR.
28944737	4	22	theme	3-hydroxy-3-methylglutaryl-coenzyme	728:762	arg1	reductase					766:774	3-hydroxy-3-methylglutaryl-coenzyme A reductase	728:774	3-hydroxy-3-methylglutaryl-coenzyme A reductase	728:774	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	1	23	theme	lovastatin	200:209	arg1	release					189:195	a delayed release	179:195	a delayed release of lovastatin from red yeast rice (RYR)	179:235	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
28944737	0	24	theme	release	50:56	arg1	profile					58:64	lovastatin release profile	39:64	lovastatin release profile from red yeast rice for hypercholesterolemia therapy	39:117	Alginate-gelatin formulation to modify lovastatin release profile from red yeast rice for hypercholesterolemia therapy.
28944737	5	25	theme	lovastatin	946:955	arg1	profile					965:971	a lovastatin release profile	944:971	a lovastatin release profile compatible with cholesterol biosynthesis	944:1012	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	0	26	from	rice	81:84	arg1	profile					58:64	lovastatin release profile	39:64	lovastatin release profile from red yeast rice for hypercholesterolemia therapy	39:117	Alginate-gelatin formulation to modify lovastatin release profile from red yeast rice for hypercholesterolemia therapy.
28944737	5	27	theme	physicochemical	900:914	arg1	properties					916:925	physicochemical properties	900:925	physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis	900:1012	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	3	28	theme	composition	443:453	arg1	effect					423:428	The effect	419:428	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices	419:526	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices was analyzed.
28944737	0	29	theme	lovastatin	39:48	arg1	profile					58:64	lovastatin release profile	39:64	lovastatin release profile from red yeast rice for hypercholesterolemia therapy	39:117	Alginate-gelatin formulation to modify lovastatin release profile from red yeast rice for hypercholesterolemia therapy.
28944737	5	30	with	compatible	973:982	arg1	biosynthesis					1001:1012	cholesterol biosynthesis	989:1012	cholesterol biosynthesis	989:1012	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	1	31	theme	red	216:218	arg1	RYR					232:234	RYR	232:234	RYR	232:234	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
28944737	1	31	theme	red	216:218	arg1	rice					226:229	red yeast rice	216:229	red yeast rice (RYR)	216:235	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
28944737	3	32	theme	swelling	480:487	arg1	behavior					489:496	swelling behavior	480:496	swelling behavior	480:496	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices was analyzed.
28944737	4	33	theme	RESULTS	542:548	arg1	Formulations					550:561	RESULTS Formulations	542:561	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50)	542:634	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	1	34	theme	system	154:159	arg1	preparation					128:138	The preparation	124:138	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR)	120:235	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
28944737	4	35	theme	prolonged	691:699	arg1	activity					712:719	a prolonged inhibitory activity	689:719	a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase	689:774	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	4	36	dep	60/40	619:623	arg1	i.e.					613:616	i.e.	613:616	i.e.	613:616	RESULTS Formulations obtained combining polymers in comparable amount (i.e., 60/40 and 50/50) guaranteed a delayed release of lovastatin from RYR, a prolonged inhibitory activity toward 3-hydroxy-3-methylglutaryl-coenzyme A reductase and a decreased cholesterol synthesis.
28944737	2	37	dep	METHODS	314:320	arg1	formulations					332:343	Polymeric formulations	322:343	MATERIALS & METHODS Polymeric formulations	302:343	MATERIALS & METHODS Polymeric formulations were prepared mixing alginate and gelatin, in different ratios, with RYR.
28944737	5	38	theme	cholesterol	989:999	arg1	biosynthesis					1001:1012	cholesterol biosynthesis	989:1012	cholesterol biosynthesis	989:1012	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	1	39	theme	yeast	220:224	arg1	RYR					232:234	RYR	232:234	RYR	232:234	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
28944737	1	39	theme	yeast	220:224	arg1	rice					226:229	red yeast rice	216:229	red yeast rice (RYR)	216:235	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
28944737	1	40	from	rice	226:229	arg1	release					189:195	a delayed release	179:195	a delayed release of lovastatin from red yeast rice (RYR)	179:235	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
28944737	2	41	theme	Polymeric	322:330	arg1	formulations					332:343	Polymeric formulations	322:343	MATERIALS & METHODS Polymeric formulations	302:343	MATERIALS & METHODS Polymeric formulations were prepared mixing alginate and gelatin, in different ratios, with RYR.
28944737	5	42	theme	release	957:963	arg1	profile					965:971	a lovastatin release profile	944:971	a lovastatin release profile compatible with cholesterol biosynthesis	944:1012	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	0	43	theme	yeast	75:79	arg1	rice					81:84	red yeast rice	71:84	red yeast rice	71:84	Alginate-gelatin formulation to modify lovastatin release profile from red yeast rice for hypercholesterolemia therapy.
28944737	5	44	theme	suitable	927:934	arg1	properties					916:925	physicochemical properties	900:925	physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis	900:1012	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	3	45	from	effect	423:428	arg1	mesostructure					502:514	mesostructure	502:514	mesostructure	502:514	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices was analyzed.
28944737	3	45	from	effect	423:428	arg1	viscosity					469:477	viscosity	469:477	viscosity	469:477	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices was analyzed.
28944737	3	45	from	effect	423:428	arg1	behavior					489:496	swelling behavior	480:496	swelling behavior	480:496	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices was analyzed.
28944737	3	45	from	effect	423:428	arg1	stiffness					458:466	stiffness	458:466	stiffness	458:466	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices was analyzed.
28944737	1	46	theme	delivery	145:152	arg1	system					154:159	a delivery system	143:159	a delivery system	143:159	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
28944737	3	47	theme	different	433:441	arg1	composition					443:453	different composition	433:453	different composition	433:453	The effect of different composition on stiffness, viscosity, swelling behavior and mesostructure of matrices was analyzed.
28944737	0	48	theme	red	71:73	arg1	rice					81:84	red yeast rice	71:84	red yeast rice	71:84	Alginate-gelatin formulation to modify lovastatin release profile from red yeast rice for hypercholesterolemia therapy.
28944737	5	49	theme	%	863:863	arg1	gelatin					865:871	60% gelatin	861:871	60% gelatin	861:871	CONCLUSION The formulation obtained combining 60% gelatin and 40% of alginate showed physicochemical properties suitable to lead a lovastatin release profile compatible with cholesterol biosynthesis.
28944737	1	50	dep	AIM	120:122	arg1	preparation					128:138	The preparation	124:138	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR)	120:235	AIM The preparation of a delivery system able to guarantee a delayed release of lovastatin from red yeast rice (RYR) is mandatory to counteract cholesterol biosynthesis effectively.
27979210	1	0	theme	enzymes	247:253	arg1	use					201:203	the use	197:203	the use of high temperature mashing and exogenous enzymes to ensure adequate starch conversion	197:290	Brewing lager beers from unmalted sorghum traditionally requires the use of high temperature mashing and exogenous enzymes to ensure adequate starch conversion.
27979210	3	1	theme	traditional	592:602	arg1	protocol					626:633	a traditional energy intensive mash protocol	590:633	a traditional energy intensive mash protocol	590:633	The low temperature mash generated worts of comparable quality to those resulting from a traditional energy intensive mash protocol.
27979210	2	2	dep	sorghums	453:460	arg1	brewing					465:471	2 brewing	463:471	2 brewing	463:471	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	2	2	dep	sorghums	453:460	arg1	varieties					491:499	3 non-brewing varieties	477:499	3 non-brewing varieties	477:499	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	3	3	theme	comparable	547:556	arg1	quality					558:564	comparable quality	547:564	comparable quality	547:564	The low temperature mash generated worts of comparable quality to those resulting from a traditional energy intensive mash protocol.
27979210	3	4	theme	low	507:509	arg1	mash					523:526	The low temperature mash	503:526	The low temperature mash	503:526	The low temperature mash generated worts of comparable quality to those resulting from a traditional energy intensive mash protocol.
27979210	7	5	theme	RVA	1082:1084	arg1	way					1110:1112	the easiest way	1098:1112	the easiest way of identifying this characteristic as potentially problematic	1098:1174	RVA profile was the easiest way of identifying this characteristic as potentially problematic.
27979210	7	5	theme	RVA	1082:1084	arg1	profile					1086:1092	RVA profile	1082:1092	RVA profile	1082:1092	RVA profile was the easiest way of identifying this characteristic as potentially problematic.
27979210	3	6	theme	temperature	511:521	arg1	mash					523:526	The low temperature mash	503:526	The low temperature mash	503:526	The low temperature mash generated worts of comparable quality to those resulting from a traditional energy intensive mash protocol.
27979210	1	7	theme	adequate	265:272	arg1	conversion					281:290	adequate starch conversion	265:290	adequate starch conversion	265:290	Brewing lager beers from unmalted sorghum traditionally requires the use of high temperature mashing and exogenous enzymes to ensure adequate starch conversion.
27979210	0	8	theme	grain	94:98	arg1	composition					100:110	sorghum grain composition	86:110	sorghum grain composition	86:110	Mashing with unmalted sorghum using a novel low temperature enzyme system: Impacts of sorghum grain composition and microstructure.
27979210	6	9	theme	interactions	1025:1036	arg1	strength					998:1005	particularly the strength	981:1005	particularly the strength of protein-starch interactions	981:1036	Rather, it was the way in which protein was structured (particularly the strength of protein-starch interactions) which most influenced brewing performance.
27979210	1	10	theme	unmalted	157:164	arg1	sorghum					166:172	unmalted sorghum	157:172	unmalted sorghum	157:172	Brewing lager beers from unmalted sorghum traditionally requires the use of high temperature mashing and exogenous enzymes to ensure adequate starch conversion.
27979210	0	11	theme	sorghum	86:92	arg1	composition					100:110	sorghum grain composition	86:110	sorghum grain composition	86:110	Mashing with unmalted sorghum using a novel low temperature enzyme system: Impacts of sorghum grain composition and microstructure.
27979210	2	12	theme	non-brewing	479:489	arg1	varieties					491:499	3 non-brewing varieties	477:499	3 non-brewing varieties	477:499	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	6	13	dep	structured	969:978	arg1	strength					998:1005	particularly the strength	981:1005	particularly the strength of protein-starch interactions	981:1036	Rather, it was the way in which protein was structured (particularly the strength of protein-starch interactions) which most influenced brewing performance.
27979210	2	14	theme	low-temperature	307:321	arg1	system					331:336	a novel low-temperature mashing system	299:336	a novel low-temperature mashing system	299:336	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	5	15	theme	lower	843:847	arg1	content					857:863	lower protein content	843:863	lower protein content	843:863	Whilst brewing sorghums were of lower protein content, protein per se did not correlate with mashing performance.
27979210	1	16	theme	starch	274:279	arg1	conversion					281:290	adequate starch conversion	265:290	adequate starch conversion	265:290	Brewing lager beers from unmalted sorghum traditionally requires the use of high temperature mashing and exogenous enzymes to ensure adequate starch conversion.
27979210	4	17	theme	raw	695:697	arg1	such					717:720	such	717:720	such	717:720	Furthermore, its performance was less dependant on sorghum raw material quality, such that it may facilitate the use of what were previously considered non-brewing varieties.
27979210	4	17	theme	raw	695:697	arg1	quality					708:714	sorghum raw material quality	687:714	sorghum raw material quality	687:714	Furthermore, its performance was less dependant on sorghum raw material quality, such that it may facilitate the use of what were previously considered non-brewing varieties.
27979210	2	18	theme	novel	301:305	arg1	system					331:336	a novel low-temperature mashing system	299:336	a novel low-temperature mashing system	299:336	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	5	19	theme	protein	849:855	arg1	content					857:863	lower protein content	843:863	lower protein content	843:863	Whilst brewing sorghums were of lower protein content, protein per se did not correlate with mashing performance.
27979210	2	20	theme	laboratory	416:425	arg1	scale					427:431	laboratory scale	416:431	laboratory scale	416:431	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	2	20	theme	laboratory	416:425	arg1	quality					398:404	the wort quality	389:404	the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties)	389:500	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	0	21	theme	unmalted	13:20	arg1	sorghum					22:28	unmalted sorghum	13:28	unmalted sorghum using a novel low temperature enzyme system	13:72	Mashing with unmalted sorghum using a novel low temperature enzyme system: Impacts of sorghum grain composition and microstructure.
27979210	0	22	theme	composition	100:110	arg1	Impacts					75:81	Impacts	75:81	Impacts of sorghum grain composition and microstructure	75:129	Mashing with unmalted sorghum using a novel low temperature enzyme system: Impacts of sorghum grain composition and microstructure.
27979210	3	23	theme	mash	621:624	arg1	protocol					626:633	a traditional energy intensive mash protocol	590:633	a traditional energy intensive mash protocol	590:633	The low temperature mash generated worts of comparable quality to those resulting from a traditional energy intensive mash protocol.
27979210	2	24	theme	quality	398:404	arg1	terms					380:384	terms	380:384	terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties)	380:500	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	5	25	theme	brewing	818:824	arg1	sorghums					826:833	brewing sorghums	818:833	brewing sorghums	818:833	Whilst brewing sorghums were of lower protein content, protein per se did not correlate with mashing performance.
27979210	6	26	theme	protein-starch	1010:1023	arg1	interactions					1025:1036	protein-starch interactions	1010:1036	protein-starch interactions	1010:1036	Rather, it was the way in which protein was structured (particularly the strength of protein-starch interactions) which most influenced brewing performance.
27979210	2	27	theme	wort	393:396	arg1	scale					427:431	laboratory scale	416:431	laboratory scale	416:431	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	2	27	theme	wort	393:396	arg1	quality					398:404	the wort quality	389:404	the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties)	389:500	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	2	28	theme	unmalted	444:451	arg1	sorghums					453:460	five unmalted sorghums	439:460	five unmalted sorghums (2 brewing and 3 non-brewing varieties)	439:500	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	0	29	theme	microstructure	116:129	arg1	Impacts					75:81	Impacts	75:81	Impacts of sorghum grain composition and microstructure	75:129	Mashing with unmalted sorghum using a novel low temperature enzyme system: Impacts of sorghum grain composition and microstructure.
27979210	3	30	theme	energy	604:609	arg1	protocol					626:633	a traditional energy intensive mash protocol	590:633	a traditional energy intensive mash protocol	590:633	The low temperature mash generated worts of comparable quality to those resulting from a traditional energy intensive mash protocol.
27979210	1	31	theme	high	208:211	arg1	mashing					225:231	high temperature mashing	208:231	high temperature mashing	208:231	Brewing lager beers from unmalted sorghum traditionally requires the use of high temperature mashing and exogenous enzymes to ensure adequate starch conversion.
27979210	0	32	theme	low	44:46	arg1	system					67:72	a novel low temperature enzyme system	36:72	a novel low temperature enzyme system	36:72	Mashing with unmalted sorghum using a novel low temperature enzyme system: Impacts of sorghum grain composition and microstructure.
27979210	3	33	theme	intensive	611:619	arg1	protocol					626:633	a traditional energy intensive mash protocol	590:633	a traditional energy intensive mash protocol	590:633	The low temperature mash generated worts of comparable quality to those resulting from a traditional energy intensive mash protocol.
27979210	2	34	dep	produced	406:413	arg1	scale					427:431	laboratory scale	416:431	laboratory scale	416:431	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	2	34	dep	produced	406:413	arg1	quality					398:404	the wort quality	389:404	the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties)	389:500	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	2	35	from	mash	372:375	arg1	terms					380:384	terms	380:384	terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties)	380:500	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	4	36	theme	sorghum	687:693	arg1	such					717:720	such	717:720	such	717:720	Furthermore, its performance was less dependant on sorghum raw material quality, such that it may facilitate the use of what were previously considered non-brewing varieties.
27979210	4	36	theme	sorghum	687:693	arg1	quality					708:714	sorghum raw material quality	687:714	sorghum raw material quality	687:714	Furthermore, its performance was less dependant on sorghum raw material quality, such that it may facilitate the use of what were previously considered non-brewing varieties.
27979210	0	37	theme	novel	38:42	arg1	system					67:72	a novel low temperature enzyme system	36:72	a novel low temperature enzyme system	36:72	Mashing with unmalted sorghum using a novel low temperature enzyme system: Impacts of sorghum grain composition and microstructure.
27979210	3	38	theme	quality	558:564	arg1	worts					538:542	worts	538:542	worts of comparable quality to those resulting from a traditional energy intensive mash protocol	538:633	The low temperature mash generated worts of comparable quality to those resulting from a traditional energy intensive mash protocol.
27979210	4	39	dep	such	717:720	arg1	facilitate					734:743	facilitate	734:743	may facilitate the use of what were previously considered non-brewing varieties	730:808	Furthermore, its performance was less dependant on sorghum raw material quality, such that it may facilitate the use of what were previously considered non-brewing varieties.
27979210	7	40	theme	easiest	1102:1108	arg1	way					1110:1112	the easiest way	1098:1112	the easiest way of identifying this characteristic as potentially problematic	1098:1174	RVA profile was the easiest way of identifying this characteristic as potentially problematic.
27979210	7	40	theme	easiest	1102:1108	arg1	profile					1086:1092	RVA profile	1082:1092	RVA profile	1082:1092	RVA profile was the easiest way of identifying this characteristic as potentially problematic.
27979210	4	41	theme	material	699:706	arg1	such					717:720	such	717:720	such	717:720	Furthermore, its performance was less dependant on sorghum raw material quality, such that it may facilitate the use of what were previously considered non-brewing varieties.
27979210	4	41	theme	material	699:706	arg1	quality					708:714	sorghum raw material quality	687:714	sorghum raw material quality	687:714	Furthermore, its performance was less dependant on sorghum raw material quality, such that it may facilitate the use of what were previously considered non-brewing varieties.
27979210	1	42	theme	temperature	213:223	arg1	mashing					225:231	high temperature mashing	208:231	high temperature mashing	208:231	Brewing lager beers from unmalted sorghum traditionally requires the use of high temperature mashing and exogenous enzymes to ensure adequate starch conversion.
27979210	2	43	dep	mash	372:375	arg1	is					338:339	is	338:339	is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties)	338:500	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	2	44	theme	mashing	323:329	arg1	system					331:336	a novel low-temperature mashing system	299:336	a novel low-temperature mashing system	299:336	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	1	45	theme	mashing	225:231	arg1	use					201:203	the use	197:203	the use of high temperature mashing and exogenous enzymes to ensure adequate starch conversion	197:290	Brewing lager beers from unmalted sorghum traditionally requires the use of high temperature mashing and exogenous enzymes to ensure adequate starch conversion.
27979210	0	46	theme	enzyme	60:65	arg1	system					67:72	a novel low temperature enzyme system	36:72	a novel low temperature enzyme system	36:72	Mashing with unmalted sorghum using a novel low temperature enzyme system: Impacts of sorghum grain composition and microstructure.
27979210	6	47	theme	brewing	1061:1067	arg1	performance					1069:1079	brewing performance	1061:1079	brewing performance	1061:1079	Rather, it was the way in which protein was structured (particularly the strength of protein-starch interactions) which most influenced brewing performance.
27979210	2	48	theme	traditional	360:370	arg1	mash					372:375	a more traditional mash	353:375	a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties)	353:500	Here, a novel low-temperature mashing system is compared to a more traditional mash in terms of the wort quality produced (laboratory scale) from five unmalted sorghums (2 brewing and 3 non-brewing varieties).
27979210	0	49	theme	temperature	48:58	arg1	system					67:72	a novel low temperature enzyme system	36:72	a novel low temperature enzyme system	36:72	Mashing with unmalted sorghum using a novel low temperature enzyme system: Impacts of sorghum grain composition and microstructure.
27979210	0	50	dep	Mashing	0:6	arg1	Impacts					75:81	Impacts	75:81	Impacts of sorghum grain composition and microstructure	75:129	Mashing with unmalted sorghum using a novel low temperature enzyme system: Impacts of sorghum grain composition and microstructure.
27979210	1	51	theme	lager	140:144	arg1	beers					146:150	lager beers	140:150	lager beers	140:150	Brewing lager beers from unmalted sorghum traditionally requires the use of high temperature mashing and exogenous enzymes to ensure adequate starch conversion.
27979210	4	52	from	dependant	674:682	arg1	such					717:720	such	717:720	such	717:720	Furthermore, its performance was less dependant on sorghum raw material quality, such that it may facilitate the use of what were previously considered non-brewing varieties.
27979210	4	52	from	dependant	674:682	arg1	quality					708:714	sorghum raw material quality	687:714	sorghum raw material quality	687:714	Furthermore, its performance was less dependant on sorghum raw material quality, such that it may facilitate the use of what were previously considered non-brewing varieties.
27979210	1	53	theme	exogenous	237:245	arg1	enzymes					247:253	exogenous enzymes	237:253	exogenous enzymes	237:253	Brewing lager beers from unmalted sorghum traditionally requires the use of high temperature mashing and exogenous enzymes to ensure adequate starch conversion.
28159225	0	0	theme	composite	73:81	arg1	scaffolds					83:91	composite scaffolds	73:91	composite scaffolds	73:91	Microwave-induced production of boron-doped HAp (B-HAp) and B-HAp coated composite scaffolds.
28159225	1	1	theme	B-HAp/Ch	289:296	arg1	scaffolds					299:307	B-HAp coated chitosan (B-HAp/Ch) scaffolds	266:307	B-HAp coated chitosan (B-HAp/Ch) scaffolds	266:307	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	10	2	attach	releasing	1568:1576	arg2	B					1566:1566	B	1566:1566	B releasing from scaffolds	1566:1591	The results of cell culture studies indicated that B releasing from scaffolds enhances proliferation and osteoblastic differentiation of MC3T3-E1 cells.
28159225	10	2	attach	releasing	1568:1576	arg1	scaffolds					1583:1591	scaffolds	1583:1591	scaffolds	1583:1591	The results of cell culture studies indicated that B releasing from scaffolds enhances proliferation and osteoblastic differentiation of MC3T3-E1 cells.
28159225	4	3	theme	exposure	646:653	arg1	times					655:659	exposure times	646:659	exposure times	646:659	Experiments were carried out with different microwave powers and exposure times, and optimum conditions for the production of B-HAp were determined.
28159225	1	4	theme	osteoinductive	190:203	arg1	property					205:212	an osteoinductive property	187:212	an osteoinductive property	187:212	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	8	5	theme	B-HAp	1397:1401	arg1	layer					1403:1407	B-HAp layer	1397:1407	B-HAp layer	1397:1407	Fourier Transform Infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) analysis also confirmed the presence of B-HAp layer.
28159225	5	6	contain	has	784:786	arg2	w/w					800:802	w/w	800:802	w/w	800:802	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	5	6	contain	has	784:786	arg2	structure					918:926	bone-like amorphous structure	898:926	bone-like amorphous structure	898:926	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	5	6	contain	has	784:786	arg2	%					840:840	4.30±0.07%	831:840	4.30±0.07% (w/w)	831:846	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	5	6	contain	has	784:786	arg2	ratio					824:828	1.40 (w/w) Ca/P ratio	808:828	1.40 (w/w) Ca/P ratio	808:828	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	5	6	contain	has	784:786	arg2	morphology					883:892	30±4nm rod-like morphology	867:892	30±4nm rod-like morphology	867:892	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	5	6	contain	has	784:786	arg2	%					797:797	1.15±0.11%	788:797	1.15±0.11% (w/w) B	788:805	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	5	6	contain	has	784:786	arg1	B-HAp					730:734	B-HAp	730:734	B-HAp precipitated from B-SBF by 600W microwave power	730:782	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	3	7	with	substitution	519:530	arg1	phosphate					552:560	phosphate	552:560	phosphate	552:560	Boron incorporation into HAp structure was performed by the substitution of borate ions with phosphate and hydroxyl ions.
28159225	3	7	with	substitution	519:530	arg1	ions					575:578	hydroxyl ions	566:578	hydroxyl ions	566:578	Boron incorporation into HAp structure was performed by the substitution of borate ions with phosphate and hydroxyl ions.
28159225	1	8	theme	scaffolds	299:307	arg1	potential					253:261	in-vitro osteogenesis potential	231:261	in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds	231:307	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	10	9	theme	osteoblastic	1620:1631	arg1	differentiation					1633:1647	osteoblastic differentiation	1620:1647	osteoblastic differentiation	1620:1647	The results of cell culture studies indicated that B releasing from scaffolds enhances proliferation and osteoblastic differentiation of MC3T3-E1 cells.
28159225	4	10	theme	microwave	625:633	arg1	powers					635:640	different microwave powers	615:640	different microwave powers	615:640	Experiments were carried out with different microwave powers and exposure times, and optimum conditions for the production of B-HAp were determined.
28159225	3	11	theme	HAp	484:486	arg1	structure					488:496	HAp structure	484:496	HAp structure	484:496	Boron incorporation into HAp structure was performed by the substitution of borate ions with phosphate and hydroxyl ions.
28159225	5	12	theme	rod-like	874:881	arg1	morphology					883:892	30±4nm rod-like morphology	867:892	30±4nm rod-like morphology	867:892	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	11	13	theme	in-vitro	1755:1762	arg1	applications					1788:1799	in-vitro bone tissue engineering applications	1755:1799	in-vitro bone tissue engineering applications	1755:1799	This work emphasized the importance of the use of B within the scaffolds for enhancing in-vitro bone tissue engineering applications.
28159225	5	14	theme	carbonate	848:856	arg1	content					858:864	carbonate content	848:864	carbonate content	848:864	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	11	15	theme	tissue	1769:1774	arg1	applications					1788:1799	in-vitro bone tissue engineering applications	1755:1799	in-vitro bone tissue engineering applications	1755:1799	This work emphasized the importance of the use of B within the scaffolds for enhancing in-vitro bone tissue engineering applications.
28159225	11	16	theme	bone	1764:1767	arg1	applications					1788:1799	in-vitro bone tissue engineering applications	1755:1799	in-vitro bone tissue engineering applications	1755:1799	This work emphasized the importance of the use of B within the scaffolds for enhancing in-vitro bone tissue engineering applications.
28159225	6	17	theme	scaffolds	1079:1087	arg1	presence					1067:1074	the presence	1063:1074	the presence of scaffolds to improve their bioactivities and mechanical properties	1063:1144	Then, chitosan scaffolds that were prepared by freeze-drying were coated with B-HAp by performing microwave-assisted precipitation in the presence of scaffolds to improve their bioactivities and mechanical properties.
28159225	8	18	dep	Fourier	1278:1284	arg1	Transform					1286:1294	Transform	1286:1294	Transform Infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) analysis	1286:1364	Fourier Transform Infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) analysis also confirmed the presence of B-HAp layer.
28159225	7	19	theme	scanning	1242:1249	arg1	SEM					1272:1274	SEM	1272:1274	SEM	1272:1274	The formation of apatite layer and the penetration of apatites into the pores were observed by scanning electron microscopy (SEM).
28159225	7	19	theme	scanning	1242:1249	arg1	microscopy					1260:1269	scanning electron microscopy	1242:1269	scanning electron microscopy (SEM)	1242:1275	The formation of apatite layer and the penetration of apatites into the pores were observed by scanning electron microscopy (SEM).
28159225	2	20	theme	microwave	441:449	arg1	energy					451:456	microwave energy	441:456	microwave energy	441:456	At first, B-HAp was produced by the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy.
28159225	0	21	theme	Microwave-induced	0:16	arg1	production					18:27	Microwave-induced production	0:27	Microwave-induced production of boron-doped HAp (B-HAp) and B-HAp	0:64	Microwave-induced production of boron-doped HAp (B-HAp) and B-HAp coated composite scaffolds.
28159225	7	22	theme	electron	1251:1258	arg1	SEM					1272:1274	SEM	1272:1274	SEM	1272:1274	The formation of apatite layer and the penetration of apatites into the pores were observed by scanning electron microscopy (SEM).
28159225	7	22	theme	electron	1251:1258	arg1	microscopy					1260:1269	scanning electron microscopy	1242:1269	scanning electron microscopy (SEM)	1242:1275	The formation of apatite layer and the penetration of apatites into the pores were observed by scanning electron microscopy (SEM).
28159225	2	23	theme	synthetic	390:398	arg1	fluid					405:409	the concentrated synthetic body fluid	373:409	the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy	373:456	At first, B-HAp was produced by the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy.
28159225	5	24	dep	%	797:797	arg1	content					858:864	carbonate content	848:864	carbonate content	848:864	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	5	24	dep	%	797:797	arg1	B					805:805	B	805:805	1.15±0.11% (w/w) B	788:805	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	4	25	theme	different	615:623	arg1	powers					635:640	different microwave powers	615:640	different microwave powers	615:640	Experiments were carried out with different microwave powers and exposure times, and optimum conditions for the production of B-HAp were determined.
28159225	2	26	theme	concentrated	377:388	arg1	fluid					405:409	the concentrated synthetic body fluid	373:409	the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy	373:456	At first, B-HAp was produced by the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy.
28159225	2	27	contain	containing	411:420	arg2	boron					422:426	boron	422:426	boron (B-SBF) with microwave energy	422:456	At first, B-HAp was produced by the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy.
28159225	2	27	contain	containing	411:420	arg1	fluid					405:409	the concentrated synthetic body fluid	373:409	the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy	373:456	At first, B-HAp was produced by the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy.
28159225	2	27	contain	containing	411:420	arg2	B-SBF					429:433	B-SBF	429:433	B-SBF	429:433	At first, B-HAp was produced by the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy.
28159225	1	28	dep	boron	137:141	arg1	B-HAp					169:173	B-HAp	169:173	B-HAp	169:173	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	1	28	dep	boron	137:141	arg1	B					144:144	B	144:144	B	144:144	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	1	28	dep	boron	137:141	arg1	hydroxyapatite					153:166	doped hydroxyapatite	147:166	doped hydroxyapatite (B-HAp)	147:174	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	4	29	theme	B-HAp	707:711	arg1	production					693:702	the production	689:702	the production of B-HAp	689:711	Experiments were carried out with different microwave powers and exposure times, and optimum conditions for the production of B-HAp were determined.
28159225	1	30	theme	doped	147:151	arg1	B-HAp					169:173	B-HAp	169:173	B-HAp	169:173	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	1	30	theme	doped	147:151	arg1	hydroxyapatite					153:166	doped hydroxyapatite	147:166	doped hydroxyapatite (B-HAp)	147:174	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	0	31	theme	boron-doped	32:42	arg1	B-HAp					49:53	B-HAp	49:53	B-HAp	49:53	Microwave-induced production of boron-doped HAp (B-HAp) and B-HAp coated composite scaffolds.
28159225	0	31	theme	boron-doped	32:42	arg1	HAp					44:46	boron-doped HAp	32:46	boron-doped HAp (B-HAp)	32:54	Microwave-induced production of boron-doped HAp (B-HAp) and B-HAp coated composite scaffolds.
28159225	1	32	theme	in-vitro	231:238	arg1	potential					253:261	in-vitro osteogenesis potential	231:261	in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds	231:307	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	10	33	theme	culture	1535:1541	arg1	studies					1543:1549	cell culture studies	1530:1549	cell culture studies	1530:1549	The results of cell culture studies indicated that B releasing from scaffolds enhances proliferation and osteoblastic differentiation of MC3T3-E1 cells.
28159225	8	34	theme	Infrared	1296:1303	arg1	spectroscopy					1305:1316	Infrared spectroscopy	1296:1316	Infrared spectroscopy (ATR-FTIR)	1296:1327	Fourier Transform Infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) analysis also confirmed the presence of B-HAp layer.
28159225	5	35	theme	bone-like	898:906	arg1	structure					918:926	bone-like amorphous structure	898:926	bone-like amorphous structure	898:926	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	2	36	theme	ions	361:364	arg1	interaction					346:356	the interaction	342:356	the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy	342:456	At first, B-HAp was produced by the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy.
28159225	5	37	theme	microwave	768:776	arg1	power					778:782	600W microwave power	763:782	600W microwave power	763:782	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	1	38	theme	osteogenesis	240:251	arg1	potential					253:261	in-vitro osteogenesis potential	231:261	in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds	231:307	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	10	39	theme	MC3T3-E1	1652:1659	arg1	cells					1661:1665	MC3T3-E1 cells	1652:1665	MC3T3-E1 cells	1652:1665	The results of cell culture studies indicated that B releasing from scaffolds enhances proliferation and osteoblastic differentiation of MC3T3-E1 cells.
28159225	7	40	theme	apatite	1164:1170	arg1	layer					1172:1176	apatite layer	1164:1176	apatite layer	1164:1176	The formation of apatite layer and the penetration of apatites into the pores were observed by scanning electron microscopy (SEM).
28159225	9	41	theme	coated	1437:1442	arg1	HAp/Ch					1464:1469	HAp/Ch	1464:1469	HAp/Ch	1464:1469	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	9	41	theme	coated	1437:1442	arg1	scaffolds					1453:1461	hydroxyapatite coated chitosan scaffolds	1422:1461	hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions	1422:1502	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	9	41	theme	coated	1437:1442	arg1	control					1413:1419	control	1413:1419	control	1413:1419	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	6	42	theme	chitosan	935:942	arg1	scaffolds					944:952	chitosan scaffolds	935:952	chitosan scaffolds that were prepared by freeze-drying	935:988	Then, chitosan scaffolds that were prepared by freeze-drying were coated with B-HAp by performing microwave-assisted precipitation in the presence of scaffolds to improve their bioactivities and mechanical properties.
28159225	7	43	theme	apatites	1201:1208	arg1	penetration					1186:1196	the penetration	1182:1196	the penetration of apatites into the pores	1182:1223	The formation of apatite layer and the penetration of apatites into the pores were observed by scanning electron microscopy (SEM).
28159225	7	43	theme	apatites	1201:1208	arg1	formation					1151:1159	The formation	1147:1159	The formation of apatite layer	1147:1176	The formation of apatite layer and the penetration of apatites into the pores were observed by scanning electron microscopy (SEM).
28159225	3	44	theme	borate	535:540	arg1	ions					542:545	borate ions	535:545	borate ions	535:545	Boron incorporation into HAp structure was performed by the substitution of borate ions with phosphate and hydroxyl ions.
28159225	10	45	theme	cell	1530:1533	arg1	studies					1543:1549	cell culture studies	1530:1549	cell culture studies	1530:1549	The results of cell culture studies indicated that B releasing from scaffolds enhances proliferation and osteoblastic differentiation of MC3T3-E1 cells.
28159225	9	46	theme	hydroxyapatite	1422:1435	arg1	HAp/Ch					1464:1469	HAp/Ch	1464:1469	HAp/Ch	1464:1469	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	9	46	theme	hydroxyapatite	1422:1435	arg1	scaffolds					1453:1461	hydroxyapatite coated chitosan scaffolds	1422:1461	hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions	1422:1502	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	9	46	theme	hydroxyapatite	1422:1435	arg1	control					1413:1419	control	1413:1419	control	1413:1419	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	0	47	theme	HAp	44:46	arg1	production					18:27	Microwave-induced production	0:27	Microwave-induced production of boron-doped HAp (B-HAp) and B-HAp	0:64	Microwave-induced production of boron-doped HAp (B-HAp) and B-HAp coated composite scaffolds.
28159225	11	48	theme	engineering	1776:1786	arg1	applications					1788:1799	in-vitro bone tissue engineering applications	1755:1799	in-vitro bone tissue engineering applications	1755:1799	This work emphasized the importance of the use of B within the scaffolds for enhancing in-vitro bone tissue engineering applications.
28159225	1	49	contain	has	183:185	arg2	property					205:212	an osteoinductive property	187:212	an osteoinductive property	187:212	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	1	49	contain	has	183:185	arg1	which					177:181	which	177:181	which	177:181	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	5	50	theme	600W	763:766	arg1	power					778:782	600W microwave power	763:782	600W microwave power	763:782	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	8	51	dep	spectroscopy	1305:1316	arg1	ATR-FTIR					1319:1326	ATR-FTIR	1319:1326	ATR-FTIR	1319:1326	Fourier Transform Infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) analysis also confirmed the presence of B-HAp layer.
28159225	1	52	theme	B-HAp	266:270	arg1	scaffolds					299:307	B-HAp coated chitosan (B-HAp/Ch) scaffolds	266:307	B-HAp coated chitosan (B-HAp/Ch) scaffolds	266:307	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	11	53	theme	B	1718:1718	arg1	use					1711:1713	the use	1707:1713	the use of B	1707:1718	This work emphasized the importance of the use of B within the scaffolds for enhancing in-vitro bone tissue engineering applications.
28159225	6	54	theme	microwave-assisted	1027:1044	arg1	precipitation					1046:1058	microwave-assisted precipitation	1027:1058	microwave-assisted precipitation	1027:1058	Then, chitosan scaffolds that were prepared by freeze-drying were coated with B-HAp by performing microwave-assisted precipitation in the presence of scaffolds to improve their bioactivities and mechanical properties.
28159225	2	55	theme	body	400:403	arg1	fluid					405:409	the concentrated synthetic body fluid	373:409	the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy	373:456	At first, B-HAp was produced by the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy.
28159225	1	56	theme	coated	272:277	arg1	scaffolds					299:307	B-HAp coated chitosan (B-HAp/Ch) scaffolds	266:307	B-HAp coated chitosan (B-HAp/Ch) scaffolds	266:307	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	9	57	theme	chitosan	1444:1451	arg1	HAp/Ch					1464:1469	HAp/Ch	1464:1469	HAp/Ch	1464:1469	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	9	57	theme	chitosan	1444:1451	arg1	scaffolds					1453:1461	hydroxyapatite coated chitosan scaffolds	1422:1461	hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions	1422:1502	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	9	57	theme	chitosan	1444:1451	arg1	control					1413:1419	control	1413:1419	control	1413:1419	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	5	58	theme	Ca/P	819:822	arg1	%					797:797	1.15±0.11%	788:797	1.15±0.11% (w/w) B	788:805	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	5	58	theme	Ca/P	819:822	arg1	ratio					824:828	1.40 (w/w) Ca/P ratio	808:828	1.40 (w/w) Ca/P ratio	808:828	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	8	59	theme	X-ray	1333:1337	arg1	XRD					1352:1354	XRD	1352:1354	XRD	1352:1354	Fourier Transform Infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) analysis also confirmed the presence of B-HAp layer.
28159225	8	59	theme	X-ray	1333:1337	arg1	diffraction					1339:1349	X-ray diffraction	1333:1349	X-ray diffraction (XRD) analysis	1333:1364	Fourier Transform Infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) analysis also confirmed the presence of B-HAp layer.
28159225	1	60	theme	chitosan	279:286	arg1	scaffolds					299:307	B-HAp coated chitosan (B-HAp/Ch) scaffolds	266:307	B-HAp coated chitosan (B-HAp/Ch) scaffolds	266:307	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	5	61	theme	amorphous	908:916	arg1	structure					918:926	bone-like amorphous structure	898:926	bone-like amorphous structure	898:926	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	6	62	theme	mechanical	1124:1133	arg1	properties					1135:1144	mechanical properties	1124:1144	mechanical properties	1124:1144	Then, chitosan scaffolds that were prepared by freeze-drying were coated with B-HAp by performing microwave-assisted precipitation in the presence of scaffolds to improve their bioactivities and mechanical properties.
28159225	5	63	theme	30±4nm	867:872	arg1	morphology					883:892	30±4nm rod-like morphology	867:892	30±4nm rod-like morphology	867:892	B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure.
28159225	3	64	theme	ions	542:545	arg1	substitution					519:530	the substitution	515:530	the substitution of borate ions with phosphate and hydroxyl ions	515:578	Boron incorporation into HAp structure was performed by the substitution of borate ions with phosphate and hydroxyl ions.
28159225	1	65	theme	present	109:115	arg1	study					117:121	the present study	105:121	the present study	105:121	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	10	66	theme	cells	1661:1665	arg1	proliferation					1602:1614	proliferation	1602:1614	proliferation	1602:1614	The results of cell culture studies indicated that B releasing from scaffolds enhances proliferation and osteoblastic differentiation of MC3T3-E1 cells.
28159225	10	66	theme	cells	1661:1665	arg1	differentiation					1633:1647	osteoblastic differentiation	1620:1647	osteoblastic differentiation	1620:1647	The results of cell culture studies indicated that B releasing from scaffolds enhances proliferation and osteoblastic differentiation of MC3T3-E1 cells.
28159225	11	67	theme	use	1711:1713	arg1	importance					1693:1702	the importance	1689:1702	the importance of the use of B	1689:1718	This work emphasized the importance of the use of B within the scaffolds for enhancing in-vitro bone tissue engineering applications.
28159225	3	68	theme	hydroxyl	566:573	arg1	ions					575:578	hydroxyl ions	566:578	hydroxyl ions	566:578	Boron incorporation into HAp structure was performed by the substitution of borate ions with phosphate and hydroxyl ions.
28159225	9	69	used	used	1509:1512	arg2	HAp/Ch					1464:1469	HAp/Ch	1464:1469	HAp/Ch	1464:1469	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	9	69	used	used	1509:1512	arg2	control					1413:1419	control	1413:1419	control	1413:1419	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	9	69	used	used	1509:1512	arg2	scaffolds					1453:1461	hydroxyapatite coated chitosan scaffolds	1422:1461	hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions	1422:1502	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	1	70	theme	study	117:121	arg1	aim					98:100	The aim	94:100	The aim of the present study	94:121	The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds.
28159225	4	71	theme	optimum	666:672	arg1	conditions					674:683	optimum conditions	666:683	optimum conditions for the production of B-HAp	666:711	Experiments were carried out with different microwave powers and exposure times, and optimum conditions for the production of B-HAp were determined.
28159225	0	72	theme	B-HAp	60:64	arg1	production					18:27	Microwave-induced production	0:27	Microwave-induced production of boron-doped HAp (B-HAp) and B-HAp	0:64	Microwave-induced production of boron-doped HAp (B-HAp) and B-HAp coated composite scaffolds.
28159225	7	73	theme	layer	1172:1176	arg1	penetration					1186:1196	the penetration	1182:1196	the penetration of apatites into the pores	1182:1223	The formation of apatite layer and the penetration of apatites into the pores were observed by scanning electron microscopy (SEM).
28159225	7	73	theme	layer	1172:1176	arg1	formation					1151:1159	The formation	1147:1159	The formation of apatite layer	1147:1176	The formation of apatite layer and the penetration of apatites into the pores were observed by scanning electron microscopy (SEM).
28159225	10	74	theme	studies	1543:1549	arg1	results					1519:1525	The results	1515:1525	The results of cell culture studies	1515:1549	The results of cell culture studies indicated that B releasing from scaffolds enhances proliferation and osteoblastic differentiation of MC3T3-E1 cells.
28159225	9	75	theme	same	1488:1491	arg1	conditions					1493:1502	the same conditions	1484:1502	the same conditions	1484:1502	As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used.
28159225	8	76	theme	layer	1403:1407	arg1	presence					1385:1392	the presence	1381:1392	the presence of B-HAp layer	1381:1407	Fourier Transform Infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) analysis also confirmed the presence of B-HAp layer.
28159225	8	77	theme	diffraction	1339:1349	arg1	analysis					1357:1364	X-ray diffraction (XRD) analysis	1333:1364	X-ray diffraction (XRD) analysis	1333:1364	Fourier Transform Infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) analysis also confirmed the presence of B-HAp layer.
28159225	2	78	with	boron	422:426	arg1	energy					451:456	microwave energy	441:456	microwave energy	441:456	At first, B-HAp was produced by the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy.
28159225	3	79	theme	Boron	459:463	arg1	incorporation					465:477	Boron incorporation	459:477	Boron incorporation into HAp structure	459:496	Boron incorporation into HAp structure was performed by the substitution of borate ions with phosphate and hydroxyl ions.
26271359	12	0	theme	microbial	1813:1821	arg1	acids					1796:1800	short chain fatty acids	1778:1800	short chain fatty acids (i.e., gut microbial metabolites)	1778:1834	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	12	0	theme	microbial	1813:1821	arg1	metabolites					1823:1833	gut microbial metabolites	1809:1833	gut microbial metabolites	1809:1833	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	12	0	theme	microbial	1813:1821	arg1	acids					1877:1881	acetic, propionic and butyric acids	1847:1881	acetic, propionic and butyric acids	1847:1881	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	14	1	theme	2.5	2104:2106	arg1	%					2107:2107	%	2107:2107	%	2107:2107	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	6	2	theme	rRNA	839:842	arg1	array					871:875	a 16S rRNA sequencing-based Phylochip array	833:875	a 16S rRNA sequencing-based Phylochip array	833:875	The colonic microbiome was profiled with a 16S rRNA sequencing-based Phylochip array.
26271359	7	3	theme	fatty	908:912	arg1	SCFAs					921:925	SCFAs	921:925	SCFAs	921:925	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	7	3	theme	fatty	908:912	arg1	acids					914:918	short chain fatty acids	896:918	short chain fatty acids (SCFAs)	896:926	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	1	4	theme	beneficial	275:284	arg1	groups					301:306	both beneficial and pathogenic groups	270:306	both beneficial and pathogenic groups	270:306	BACKGROUND Gastrointestinal microbial communities are diverse and are composed of both beneficial and pathogenic groups.
26271359	12	5	theme	fatty	1790:1794	arg1	acids					1796:1800	short chain fatty acids	1778:1800	short chain fatty acids (i.e., gut microbial metabolites)	1778:1834	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	12	5	theme	fatty	1790:1794	arg1	metabolites					1823:1833	gut microbial metabolites	1809:1833	gut microbial metabolites	1809:1833	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	12	5	theme	fatty	1790:1794	arg1	acids					1877:1881	acetic, propionic and butyric acids	1847:1881	acetic, propionic and butyric acids	1847:1881	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	13	6	theme	significant	2003:2013	arg1	improvements					2015:2026	significant improvements	2003:2026	significant improvements in proximal colon histo-morphology	2003:2061	Furthermore, both C. crispus and FOS supplemented rats showed significant improvements in proximal colon histo-morphology.
26271359	4	7	theme	Prebiotic	606:614	arg1	effects					616:622	Prebiotic effects	606:622	Prebiotic effects	606:622	METHODS Prebiotic effects were investigated with weaning rats fed a cultivated C. crispus-supplemented diet.
26271359	10	8	theme	2.5	1422:1424	arg1	%					1425:1425	%	1425:1425	%	1425:1425	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	7	9	theme	short	896:900	arg1	SCFAs					921:925	SCFAs	921:925	SCFAs	921:925	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	7	9	theme	short	896:900	arg1	acids					914:918	short chain fatty acids	896:918	short chain fatty acids (SCFAs)	896:926	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	1	10	theme	pathogenic	290:299	arg1	groups					301:306	both beneficial and pathogenic groups	270:306	both beneficial and pathogenic groups	270:306	BACKGROUND Gastrointestinal microbial communities are diverse and are composed of both beneficial and pathogenic groups.
26271359	11	11	dep	increased	1614:1622	arg1	p=0.001					1635:1641	p=0.001	1635:1641	p=0.001	1635:1641	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	11	11	dep	increased	1614:1622	arg1	4.9-fold					1625:1632	4.9-fold	1625:1632	4.9-fold	1625:1632	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	4	12	theme	C.	677:678	arg1	diet					701:704	a cultivated C. crispus-supplemented diet	664:704	a cultivated C. crispus-supplemented diet	664:704	METHODS Prebiotic effects were investigated with weaning rats fed a cultivated C. crispus-supplemented diet.
26271359	11	13	theme	pathogenic	1666:1675	arg1	species					1677:1683	pathogenic species	1666:1683	pathogenic species such as Clostridium septicum and Streptococcus pneumonia	1666:1740	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	11	13	theme	pathogenic	1666:1675	arg1	septicum					1705:1712	Clostridium septicum	1693:1712	Clostridium septicum	1693:1712	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	11	13	theme	pathogenic	1666:1675	arg1	pneumonia					1732:1740	Streptococcus pneumonia	1718:1740	Streptococcus pneumonia	1718:1740	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	3	14	from	rich	492:495	arg1	oligosaccharides					518:533	oligosaccharides	518:533	oligosaccharides	518:533	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	3	14	from	rich	492:495	arg1	fiber					508:512	dietary fiber	500:512	dietary fiber	500:512	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	3	15	theme	prebiotic	548:556	arg1	potential					558:566	its prebiotic potential	544:566	its prebiotic potential	544:566	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	11	16	theme	bacteria	1575:1582	arg1	population					1550:1559	the population	1546:1559	the population of beneficial bacteria such as Bifidobacterium breve	1546:1612	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	2	17	theme	digestion-resistant	329:347	arg1	fibers					349:354	digestion-resistant fibers	329:354	digestion-resistant fibers	329:354	Prebiotics, such as digestion-resistant fibers, influence the composition of gut microbiota, and can contribute to the improvement of host health.
26271359	1	18	theme	Gastrointestinal	199:214	arg1	communities					226:236	BACKGROUND Gastrointestinal microbial communities	188:236	BACKGROUND Gastrointestinal microbial communities	188:236	BACKGROUND Gastrointestinal microbial communities are diverse and are composed of both beneficial and pathogenic groups.
26271359	15	19	theme	cultivated	2464:2473	arg1	crispus					2478:2484	cultivated C. crispus	2464:2484	cultivated C. crispus	2464:2484	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	12	20	theme	short	1778:1782	arg1	acids					1796:1800	short chain fatty acids	1778:1800	short chain fatty acids (i.e., gut microbial metabolites)	1778:1834	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	12	20	theme	short	1778:1782	arg1	metabolites					1823:1833	gut microbial metabolites	1809:1833	gut microbial metabolites	1809:1833	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	12	20	theme	short	1778:1782	arg1	acids					1877:1881	acetic, propionic and butyric acids	1847:1881	acetic, propionic and butyric acids	1847:1881	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	13	21	theme	C.	1959:1960	arg1	crispus					1962:1968	C. crispus	1959:1968	C. crispus	1959:1968	Furthermore, both C. crispus and FOS supplemented rats showed significant improvements in proximal colon histo-morphology.
26271359	5	22	theme	fructo-oligo-saccharide	739:761	arg1	diet					769:772	a fructo-oligo-saccharide (FOS) diet	737:772	a fructo-oligo-saccharide (FOS) diet	737:772	Comparison standards included a fructo-oligo-saccharide (FOS) diet and a basal diet.
26271359	10	23	from	control	1479:1485	arg1	separation					1459:1468	larger separation	1452:1468	larger separation from the control than other treatment groups	1452:1513	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	11	24	theme	beneficial	1564:1573	arg1	breve					1608:1612	Bifidobacterium breve	1592:1612	Bifidobacterium breve	1592:1612	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	11	24	theme	beneficial	1564:1573	arg1	bacteria					1575:1582	beneficial bacteria	1564:1582	beneficial bacteria such as Bifidobacterium breve	1564:1612	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	14	25	theme	immunoglobulin	2147:2160	arg1	levels					2176:2181	elevated plasma immunoglobulin (IgA and IgG) levels	2131:2181	elevated plasma immunoglobulin (IgA and IgG) levels	2131:2181	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	5	26	theme	FOS	764:766	arg1	diet					769:772	a fructo-oligo-saccharide (FOS) diet	737:772	a fructo-oligo-saccharide (FOS) diet	737:772	Comparison standards included a fructo-oligo-saccharide (FOS) diet and a basal diet.
26271359	10	27	dep	diet-supplemented	1353:1369	arg1	groups					1387:1392	groups	1387:1392	groups	1387:1392	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	9	28	theme	proximal	1185:1192	arg1	tissue					1200:1205	the proximal colon tissue	1181:1205	the proximal colon tissue	1181:1205	Histo-morphological parameters of the proximal colon tissue were characterized by hematoxylin and eosin (H&E) staining.
26271359	7	29	with	chromatography	973:986	arg1	analysis					1030:1037	a flame ionization detector (GC-FID) analysis	993:1037	a flame ionization detector (GC-FID) analysis	993:1037	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	14	30	theme	elevated	2131:2138	arg1	levels					2176:2181	elevated plasma immunoglobulin (IgA and IgG) levels	2131:2181	elevated plasma immunoglobulin (IgA and IgG) levels	2131:2181	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	5	31	theme	Comparison	707:716	arg1	standards					718:726	Comparison standards	707:726	Comparison standards	707:726	Comparison standards included a fructo-oligo-saccharide (FOS) diet and a basal diet.
26271359	7	32	theme	GC-FID	1022:1027	arg1	analysis					1030:1037	a flame ionization detector (GC-FID) analysis	993:1037	a flame ionization detector (GC-FID) analysis	993:1037	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	0	33	from	fructo-oligo-saccharide	96:118	arg1	gut					161:163	gut	161:163	gut	161:163	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	0	33	from	fructo-oligo-saccharide	96:118	arg1	immunity					128:135	host immunity	123:135	host immunity	123:135	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	0	33	from	fructo-oligo-saccharide	96:118	arg1	microbiota					146:155	colonic microbiota	138:155	colonic microbiota	138:155	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	10	34	theme	%	1425:1425	arg1	supplementation					1427:1441	2.5% supplementation	1422:1441	2.5% supplementation	1422:1441	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	10	34	theme	%	1425:1425	arg1	group					1415:1419	the C. crispus group	1400:1419	the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups	1400:1513	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	11	35	theme	%	1526:1526	arg1	group					1539:1543	the 2.5% C. crispus group	1519:1543	the 2.5% C. crispus group	1519:1543	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	15	36	theme	prebiotic	2305:2313	arg1	effects					2315:2321	multiple prebiotic effects	2296:2321	multiple prebiotic effects	2296:2321	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	7	37	theme	detector	1012:1019	arg1	analysis					1030:1037	a flame ionization detector (GC-FID) analysis	993:1037	a flame ionization detector (GC-FID) analysis	993:1037	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	12	38	theme	Higher	1753:1758	arg1	concentrations					1760:1773	Higher concentrations	1753:1773	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids,	1753:1882	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	0	39	theme	red	59:61	arg1	crispus					80:86	the cultivated red seaweed Chondrus crispus	44:86	the cultivated red seaweed Chondrus crispus	44:86	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	14	40	dep	immunoglobulin	2147:2160	arg1	IgG					2171:2173	IgG	2171:2173	IgG	2171:2173	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	14	40	dep	immunoglobulin	2147:2160	arg1	IgA					2163:2165	IgA	2163:2165	IgA	2163:2165	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	7	41	theme	flame	995:999	arg1	analysis					1030:1037	a flame ionization detector (GC-FID) analysis	993:1037	a flame ionization detector (GC-FID) analysis	993:1037	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	0	42	theme	Chondrus	71:78	arg1	crispus					80:86	the cultivated red seaweed Chondrus crispus	44:86	the cultivated red seaweed Chondrus crispus	44:86	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	8	43	theme	Immunoglobulin	1040:1053	arg1	levels					1055:1060	Immunoglobulin levels	1040:1060	Immunoglobulin levels in the blood plasma	1040:1080	Immunoglobulin levels in the blood plasma were analyzed with an enzyme-linked immunosorbent assay (ELISA).
26271359	10	44	theme	Phylochip	1275:1283	arg1	analysis					1291:1298	Phylochip array analysis	1275:1298	Phylochip array analysis	1275:1298	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	15	45	theme	immune	2420:2425	arg1	modulation					2427:2436	immune modulation	2420:2436	immune modulation in rats supplemented with cultivated C. crispus	2420:2484	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	15	46	theme	microbial	2367:2375	arg1	communities					2377:2387	gut microbial communities	2363:2387	gut microbial communities	2363:2387	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	12	47	theme	crispus-fed	1923:1933	arg1	rats					1935:1938	the C. crispus-fed rats	1916:1938	the C. crispus-fed rats	1916:1938	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	7	48	theme	gas	969:971	arg1	chromatography					973:986	gas chromatography	969:986	gas chromatography with a flame ionization detector (GC-FID) analysis	969:1037	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	14	49	theme	C.	2109:2110	arg1	group					2120:2124	the 2.5% C. crispus group	2100:2124	the 2.5% C. crispus group	2100:2124	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	8	50	theme	blood	1069:1073	arg1	plasma					1075:1080	the blood plasma	1065:1080	the blood plasma	1065:1080	Immunoglobulin levels in the blood plasma were analyzed with an enzyme-linked immunosorbent assay (ELISA).
26271359	15	51	theme	health	2409:2414	arg1	improvement					2390:2400	improvement	2390:2400	improvement of gut health	2390:2414	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	15	51	theme	health	2409:2414	arg1	modulation					2427:2436	immune modulation	2420:2436	immune modulation in rats supplemented with cultivated C. crispus	2420:2484	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	15	51	theme	health	2409:2414	arg1	composition					2348:2358	the composition	2344:2358	the composition of gut microbial communities	2344:2387	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	10	52	theme	treatment	1498:1506	arg1	groups					1508:1513	other treatment groups	1492:1513	other treatment groups	1492:1513	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	0	53	theme	seaweed	63:69	arg1	crispus					80:86	the cultivated red seaweed Chondrus crispus	44:86	the cultivated red seaweed Chondrus crispus	44:86	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	10	54	theme	differing	1310:1318	arg1	composition					1331:1341	differing microbiome composition	1310:1341	differing microbiome composition among the diet-supplemented and the control groups	1310:1392	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	0	55	theme	Prebiotic	0:8	arg1	effects					10:16	Prebiotic effects	0:16	Prebiotic effects of diet	0:24	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	10	56	dep	RESULTS	1267:1273	arg1	indicated					1300:1308	indicated	1300:1308	indicated	1300:1308	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	11	57	theme	Bifidobacterium	1592:1606	arg1	breve					1608:1612	Bifidobacterium breve	1592:1612	Bifidobacterium breve	1592:1612	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	14	58	theme	feed	2252:2255	arg1	group					2257:2261	the basal feed group	2242:2261	the basal feed group	2242:2261	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	6	59	theme	16S	835:837	arg1	array					871:875	a 16S rRNA sequencing-based Phylochip array	833:875	a 16S rRNA sequencing-based Phylochip array	833:875	The colonic microbiome was profiled with a 16S rRNA sequencing-based Phylochip array.
26271359	2	60	theme	host	443:446	arg1	health					448:453	host health	443:453	host health	443:453	Prebiotics, such as digestion-resistant fibers, influence the composition of gut microbiota, and can contribute to the improvement of host health.
26271359	8	61	link	enzyme-linked	1104:1116	arg1	ELISA					1139:1143	ELISA	1139:1143	ELISA	1139:1143	Immunoglobulin levels in the blood plasma were analyzed with an enzyme-linked immunosorbent assay (ELISA).
26271359	8	61	link	enzyme-linked	1104:1116	arg1	assay					1132:1136	an enzyme-linked immunosorbent assay	1101:1136	an enzyme-linked immunosorbent assay (ELISA)	1101:1144	Immunoglobulin levels in the blood plasma were analyzed with an enzyme-linked immunosorbent assay (ELISA).
26271359	14	62	theme	crispus	2212:2218	arg1	group					2220:2224	the 0.5% C. crispus group	2200:2224	the 0.5% C. crispus group	2200:2224	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	3	63	theme	seaweed	464:470	arg1	Chondrus					472:479	The red seaweed Chondrus crispus	456:487	The red seaweed Chondrus crispus	456:487	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	3	63	theme	seaweed	464:470	arg1	rich					492:495	rich	492:495	rich	492:495	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	8	64	theme	enzyme-linked	1104:1116	arg1	ELISA					1139:1143	ELISA	1139:1143	ELISA	1139:1143	Immunoglobulin levels in the blood plasma were analyzed with an enzyme-linked immunosorbent assay (ELISA).
26271359	8	64	theme	enzyme-linked	1104:1116	arg1	assay					1132:1136	an enzyme-linked immunosorbent assay	1101:1136	an enzyme-linked immunosorbent assay (ELISA)	1101:1144	Immunoglobulin levels in the blood plasma were analyzed with an enzyme-linked immunosorbent assay (ELISA).
26271359	14	65	theme	faecal	2071:2076	arg1	moisture					2078:2085	Higher faecal moisture	2064:2085	Higher faecal moisture	2064:2085	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	9	66	theme	Histo-morphological	1147:1165	arg1	parameters					1167:1176	Histo-morphological parameters	1147:1176	Histo-morphological parameters of the proximal colon tissue	1147:1205	Histo-morphological parameters of the proximal colon tissue were characterized by hematoxylin and eosin (H&E) staining.
26271359	3	67	theme	dietary	500:506	arg1	fiber					508:512	dietary fiber	500:512	dietary fiber	500:512	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	12	68	theme	acetic	1847:1852	arg1	acids					1877:1881	acetic, propionic and butyric acids	1847:1881	acetic, propionic and butyric acids	1847:1881	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	7	69	from	Concentrations	878:891	arg1	samples					942:948	the feacal samples	931:948	the feacal samples	931:948	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	13	70	theme	colon	2040:2044	arg1	histo-morphology					2046:2061	proximal colon histo-morphology	2031:2061	proximal colon histo-morphology	2031:2061	Furthermore, both C. crispus and FOS supplemented rats showed significant improvements in proximal colon histo-morphology.
26271359	7	71	theme	feacal	935:940	arg1	samples					942:948	the feacal samples	931:948	the feacal samples	931:948	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	6	72	theme	Phylochip	861:869	arg1	array					871:875	a 16S rRNA sequencing-based Phylochip array	833:875	a 16S rRNA sequencing-based Phylochip array	833:875	The colonic microbiome was profiled with a 16S rRNA sequencing-based Phylochip array.
26271359	3	73	dep	Chondrus	472:479	arg1	crispus					481:487	crispus	481:487	The red seaweed Chondrus crispus	456:487	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	12	74	theme	gut	1809:1811	arg1	acids					1796:1800	short chain fatty acids	1778:1800	short chain fatty acids (i.e., gut microbial metabolites)	1778:1834	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	12	74	theme	gut	1809:1811	arg1	metabolites					1823:1833	gut microbial metabolites	1809:1833	gut microbial metabolites	1809:1833	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	12	74	theme	gut	1809:1811	arg1	acids					1877:1881	acetic, propionic and butyric acids	1847:1881	acetic, propionic and butyric acids	1847:1881	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	14	75	theme	C.	2209:2210	arg1	group					2220:2224	the 0.5% C. crispus group	2200:2224	the 0.5% C. crispus group	2200:2224	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	10	76	theme	crispus	1407:1413	arg1	supplementation					1427:1441	2.5% supplementation	1422:1441	2.5% supplementation	1422:1441	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	10	76	theme	crispus	1407:1413	arg1	group					1415:1419	the C. crispus group	1400:1419	the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups	1400:1513	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	14	77	theme	0.5	2204:2206	arg1	%					2207:2207	%	2207:2207	%	2207:2207	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	9	78	theme	tissue	1200:1205	arg1	parameters					1167:1176	Histo-morphological parameters	1147:1176	Histo-morphological parameters of the proximal colon tissue	1147:1205	Histo-morphological parameters of the proximal colon tissue were characterized by hematoxylin and eosin (H&E) staining.
26271359	2	79	theme	gut	386:388	arg1	microbiota					390:399	gut microbiota	386:399	gut microbiota	386:399	Prebiotics, such as digestion-resistant fibers, influence the composition of gut microbiota, and can contribute to the improvement of host health.
26271359	12	80	theme	acids	1796:1800	arg1	concentrations					1760:1773	Higher concentrations	1753:1773	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids,	1753:1882	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	10	81	theme	larger	1452:1457	arg1	separation					1459:1468	larger separation	1452:1468	larger separation from the control than other treatment groups	1452:1513	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	3	82	from	oligosaccharides	518:533	arg1	Chondrus					472:479	The red seaweed Chondrus crispus	456:487	The red seaweed Chondrus crispus	456:487	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	3	82	from	oligosaccharides	518:533	arg1	rich					492:495	rich	492:495	rich	492:495	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	7	83	theme	acids	914:918	arg1	Concentrations					878:891	Concentrations	878:891	Concentrations of short chain fatty acids (SCFAs) in the feacal samples	878:948	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	14	84	located	observed	2188:2195	arg2	levels					2176:2181	elevated plasma immunoglobulin (IgA and IgG) levels	2131:2181	elevated plasma immunoglobulin (IgA and IgG) levels	2131:2181	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	14	84	located	observed	2188:2195	arg1	group					2220:2224	the 0.5% C. crispus group	2200:2224	the 0.5% C. crispus group	2200:2224	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	9	85	theme	hematoxylin	1229:1239	arg1	staining					1257:1264	hematoxylin and eosin (H&E) staining	1229:1264	hematoxylin and eosin (H&E) staining	1229:1264	Histo-morphological parameters of the proximal colon tissue were characterized by hematoxylin and eosin (H&E) staining.
26271359	7	86	theme	chain	902:906	arg1	SCFAs					921:925	SCFAs	921:925	SCFAs	921:925	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	7	86	theme	chain	902:906	arg1	acids					914:918	short chain fatty acids	896:918	short chain fatty acids (SCFAs)	896:926	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	4	87	theme	cultivated	666:675	arg1	diet					701:704	a cultivated C. crispus-supplemented diet	664:704	a cultivated C. crispus-supplemented diet	664:704	METHODS Prebiotic effects were investigated with weaning rats fed a cultivated C. crispus-supplemented diet.
26271359	11	88	theme	species	1677:1683	arg1	abundance					1653:1661	the abundance	1649:1661	the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia	1649:1740	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	9	89	theme	eosin	1245:1249	arg1	staining					1257:1264	hematoxylin and eosin (H&E) staining	1229:1264	hematoxylin and eosin (H&E) staining	1229:1264	Histo-morphological parameters of the proximal colon tissue were characterized by hematoxylin and eosin (H&E) staining.
26271359	1	90	theme	BACKGROUND	188:197	arg1	communities					226:236	BACKGROUND Gastrointestinal microbial communities	188:236	BACKGROUND Gastrointestinal microbial communities	188:236	BACKGROUND Gastrointestinal microbial communities are diverse and are composed of both beneficial and pathogenic groups.
26271359	12	91	theme	chain	1784:1788	arg1	acids					1796:1800	short chain fatty acids	1778:1800	short chain fatty acids (i.e., gut microbial metabolites)	1778:1834	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	12	91	theme	chain	1784:1788	arg1	metabolites					1823:1833	gut microbial metabolites	1809:1833	gut microbial metabolites	1809:1833	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	12	91	theme	chain	1784:1788	arg1	acids					1877:1881	acetic, propionic and butyric acids	1847:1881	acetic, propionic and butyric acids	1847:1881	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	4	92	theme	crispus-supplemented	680:699	arg1	diet					701:704	a cultivated C. crispus-supplemented diet	664:704	a cultivated C. crispus-supplemented diet	664:704	METHODS Prebiotic effects were investigated with weaning rats fed a cultivated C. crispus-supplemented diet.
26271359	3	93	from	fiber	508:512	arg1	Chondrus					472:479	The red seaweed Chondrus crispus	456:487	The red seaweed Chondrus crispus	456:487	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	3	93	from	fiber	508:512	arg1	rich					492:495	rich	492:495	rich	492:495	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	1	94	theme	microbial	216:224	arg1	communities					226:236	BACKGROUND Gastrointestinal microbial communities	188:236	BACKGROUND Gastrointestinal microbial communities	188:236	BACKGROUND Gastrointestinal microbial communities are diverse and are composed of both beneficial and pathogenic groups.
26271359	8	95	from	levels	1055:1060	arg1	plasma					1075:1080	the blood plasma	1065:1080	the blood plasma	1065:1080	Immunoglobulin levels in the blood plasma were analyzed with an enzyme-linked immunosorbent assay (ELISA).
26271359	4	96	theme	weaning	647:653	arg1	rats					655:658	weaning rats	647:658	weaning rats fed a cultivated C. crispus-supplemented diet	647:704	METHODS Prebiotic effects were investigated with weaning rats fed a cultivated C. crispus-supplemented diet.
26271359	0	97	theme	colonic	138:144	arg1	microbiota					146:155	colonic microbiota	138:155	colonic microbiota	138:155	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	4	98	dep	METHODS	598:604	arg1	investigated					629:640	investigated	629:640	were investigated with weaning rats fed a cultivated C. crispus-supplemented diet	624:704	METHODS Prebiotic effects were investigated with weaning rats fed a cultivated C. crispus-supplemented diet.
26271359	15	99	from	modulation	2427:2436	arg1	rats					2441:2444	rats	2441:2444	rats supplemented with cultivated C. crispus	2441:2484	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	13	100	from	improvements	2015:2026	arg1	histo-morphology					2046:2061	proximal colon histo-morphology	2031:2061	proximal colon histo-morphology	2031:2061	Furthermore, both C. crispus and FOS supplemented rats showed significant improvements in proximal colon histo-morphology.
26271359	14	101	theme	plasma	2140:2145	arg1	immunoglobulin					2147:2160	plasma immunoglobulin	2140:2160	elevated plasma immunoglobulin (IgA and IgG) levels	2131:2181	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	11	102	theme	2.5	1523:1525	arg1	%					1526:1526	%	1526:1526	%	1526:1526	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	6	103	theme	colonic	796:802	arg1	microbiome					804:813	The colonic microbiome	792:813	The colonic microbiome	792:813	The colonic microbiome was profiled with a 16S rRNA sequencing-based Phylochip array.
26271359	0	104	theme	microbial	165:173	arg1	metabolites					175:185	microbial metabolites	165:185	microbial metabolites	165:185	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	12	105	located	found	1889:1893	arg1	samples					1905:1911	faecal samples	1898:1911	faecal samples of the C. crispus-fed rats	1898:1938	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	12	105	located	found	1889:1893	arg2	concentrations					1760:1773	Higher concentrations	1753:1773	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids,	1753:1882	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	5	106	theme	basal	780:784	arg1	diet					786:789	a basal diet	778:789	a basal diet	778:789	Comparison standards included a fructo-oligo-saccharide (FOS) diet and a basal diet.
26271359	11	107	theme	crispus	1531:1537	arg1	group					1539:1543	the 2.5% C. crispus group	1519:1543	the 2.5% C. crispus group	1519:1543	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	13	108	dep	crispus	1962:1968	arg1	rats					1991:1994	supplemented rats	1978:1994	supplemented rats	1978:1994	Furthermore, both C. crispus and FOS supplemented rats showed significant improvements in proximal colon histo-morphology.
26271359	15	109	theme	gut	2405:2407	arg1	health					2409:2414	gut health	2405:2414	gut health	2405:2414	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	12	110	theme	C.	1920:1921	arg1	rats					1935:1938	the C. crispus-fed rats	1916:1938	the C. crispus-fed rats	1916:1938	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	7	111	theme	ionization	1001:1010	arg1	analysis					1030:1037	a flame ionization detector (GC-FID) analysis	993:1037	a flame ionization detector (GC-FID) analysis	993:1037	Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis.
26271359	0	112	dep	immunity	128:135	arg1	metabolites					175:185	microbial metabolites	165:185	microbial metabolites	165:185	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	0	113	theme	host	123:126	arg1	immunity					128:135	host immunity	123:135	host immunity	123:135	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	11	114	theme	C.	1528:1529	arg1	group					1539:1543	the 2.5% C. crispus group	1519:1543	the 2.5% C. crispus group	1519:1543	In the 2.5% C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p=0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased.
26271359	12	115	theme	rats	1935:1938	arg1	samples					1905:1911	faecal samples	1898:1911	faecal samples of the C. crispus-fed rats	1898:1938	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	14	116	theme	crispus	2112:2118	arg1	group					2120:2124	the 2.5% C. crispus group	2100:2124	the 2.5% C. crispus group	2100:2124	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	12	117	dep	metabolites	1823:1833	arg1	i.e.					1803:1806	i.e.	1803:1806	i.e.	1803:1806	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	14	118	theme	basal	2246:2250	arg1	group					2257:2261	the basal feed group	2242:2261	the basal feed group	2242:2261	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	12	119	theme	faecal	1898:1903	arg1	samples					1905:1911	faecal samples	1898:1911	faecal samples of the C. crispus-fed rats	1898:1938	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	15	120	dep	CONCLUSIONS	2264:2274	arg1	suggest					2288:2294	suggest	2288:2294	suggest	2288:2294	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	15	121	from	composition	2348:2358	arg1	rats					2441:2444	rats	2441:2444	rats supplemented with cultivated C. crispus	2441:2484	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	14	122	theme	%	2107:2107	arg1	group					2120:2124	the 2.5% C. crispus group	2100:2124	the 2.5% C. crispus group	2100:2124	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	10	123	theme	microbiome	1320:1329	arg1	composition					1331:1341	differing microbiome composition	1310:1341	differing microbiome composition among the diet-supplemented and the control groups	1310:1392	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	3	124	theme	red	460:462	arg1	Chondrus					472:479	The red seaweed Chondrus crispus	456:487	The red seaweed Chondrus crispus	456:487	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	3	124	theme	red	460:462	arg1	rich					492:495	rich	492:495	rich	492:495	The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date.
26271359	15	125	theme	gut	2363:2365	arg1	communities					2377:2387	gut microbial communities	2363:2387	gut microbial communities	2363:2387	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	15	126	theme	communities	2377:2387	arg1	improvement					2390:2400	improvement	2390:2400	improvement of gut health	2390:2414	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	15	126	theme	communities	2377:2387	arg1	modulation					2427:2436	immune modulation	2420:2436	immune modulation in rats supplemented with cultivated C. crispus	2420:2484	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	15	126	theme	communities	2377:2387	arg1	composition					2348:2358	the composition	2344:2358	the composition of gut microbial communities	2344:2387	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	10	127	theme	other	1492:1496	arg1	groups					1508:1513	other treatment groups	1492:1513	other treatment groups	1492:1513	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	0	128	theme	diet	21:24	arg1	effects					10:16	Prebiotic effects	0:16	Prebiotic effects of diet	0:24	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	15	129	theme	C.	2475:2476	arg1	crispus					2478:2484	cultivated C. crispus	2464:2484	cultivated C. crispus	2464:2484	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	2	130	theme	health	448:453	arg1	improvement					428:438	the improvement	424:438	the improvement of host health	424:453	Prebiotics, such as digestion-resistant fibers, influence the composition of gut microbiota, and can contribute to the improvement of host health.
26271359	8	131	theme	immunosorbent	1118:1130	arg1	ELISA					1139:1143	ELISA	1139:1143	ELISA	1139:1143	Immunoglobulin levels in the blood plasma were analyzed with an enzyme-linked immunosorbent assay (ELISA).
26271359	8	131	theme	immunosorbent	1118:1130	arg1	assay					1132:1136	an enzyme-linked immunosorbent assay	1101:1136	an enzyme-linked immunosorbent assay (ELISA)	1101:1144	Immunoglobulin levels in the blood plasma were analyzed with an enzyme-linked immunosorbent assay (ELISA).
26271359	15	132	from	improvement	2390:2400	arg1	rats					2441:2444	rats	2441:2444	rats supplemented with cultivated C. crispus	2441:2484	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	0	133	theme	cultivated	48:57	arg1	crispus					80:86	the cultivated red seaweed Chondrus crispus	44:86	the cultivated red seaweed Chondrus crispus	44:86	Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites.
26271359	12	134	theme	propionic	1855:1863	arg1	acids					1877:1881	acetic, propionic and butyric acids	1847:1881	acetic, propionic and butyric acids	1847:1881	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	14	135	theme	Higher	2064:2069	arg1	moisture					2078:2085	Higher faecal moisture	2064:2085	Higher faecal moisture	2064:2085	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	13	136	theme	proximal	2031:2038	arg1	histo-morphology					2046:2061	proximal colon histo-morphology	2031:2061	proximal colon histo-morphology	2031:2061	Furthermore, both C. crispus and FOS supplemented rats showed significant improvements in proximal colon histo-morphology.
26271359	12	137	theme	butyric	1869:1875	arg1	acids					1877:1881	acetic, propionic and butyric acids	1847:1881	acetic, propionic and butyric acids	1847:1881	Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats.
26271359	9	138	theme	colon	1194:1198	arg1	tissue					1200:1205	the proximal colon tissue	1181:1205	the proximal colon tissue	1181:1205	Histo-morphological parameters of the proximal colon tissue were characterized by hematoxylin and eosin (H&E) staining.
26271359	10	139	theme	array	1285:1289	arg1	analysis					1291:1298	Phylochip array analysis	1275:1298	Phylochip array analysis	1275:1298	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	13	140	theme	supplemented	1978:1989	arg1	rats					1991:1994	supplemented rats	1978:1994	supplemented rats	1978:1994	Furthermore, both C. crispus and FOS supplemented rats showed significant improvements in proximal colon histo-morphology.
26271359	15	141	theme	multiple	2296:2303	arg1	effects					2315:2321	multiple prebiotic effects	2296:2321	multiple prebiotic effects	2296:2321	CONCLUSIONS The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
26271359	6	142	theme	sequencing-based	844:859	arg1	array					871:875	a 16S rRNA sequencing-based Phylochip array	833:875	a 16S rRNA sequencing-based Phylochip array	833:875	The colonic microbiome was profiled with a 16S rRNA sequencing-based Phylochip array.
26271359	14	143	theme	%	2207:2207	arg1	group					2220:2224	the 0.5% C. crispus group	2200:2224	the 0.5% C. crispus group	2200:2224	Higher faecal moisture was noted in the 2.5% C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5% C. crispus group, as compared to the basal feed group.
26271359	10	144	theme	C.	1404:1405	arg1	supplementation					1427:1441	2.5% supplementation	1422:1441	2.5% supplementation	1422:1441	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	10	144	theme	C.	1404:1405	arg1	group					1415:1419	the C. crispus group	1400:1419	the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups	1400:1513	RESULTS Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5% supplementation) showing larger separation from the control than other treatment groups.
26271359	2	145	theme	microbiota	390:399	arg1	composition					371:381	the composition	367:381	the composition of gut microbiota	367:399	Prebiotics, such as digestion-resistant fibers, influence the composition of gut microbiota, and can contribute to the improvement of host health.
28884590	10	0	theme	Bifidobacteriaceae	1565:1582	arg1	lower					1646:1650	lower	1646:1650	lower	1646:1650	The abundance of Bifidobacteriaceae and the diversity of intestinal microbiota were significantly lower in patients with diabetes than in healthy subjects.
28884590	10	0	theme	Bifidobacteriaceae	1565:1582	arg1	diversity					1592:1600	the diversity	1588:1600	the diversity of intestinal microbiota	1588:1625	The abundance of Bifidobacteriaceae and the diversity of intestinal microbiota were significantly lower in patients with diabetes than in healthy subjects.
28884590	10	0	theme	Bifidobacteriaceae	1565:1582	arg1	abundance					1552:1560	The abundance	1548:1560	The abundance of Bifidobacteriaceae	1548:1582	The abundance of Bifidobacteriaceae and the diversity of intestinal microbiota were significantly lower in patients with diabetes than in healthy subjects.
28884590	3	1	theme	gut	539:541	arg1	microbiota					543:552	the gut microbiota	535:552	the gut microbiota	535:552	Several studies have reported relationships between metabolic diseases and the gut microbiota; and prebiotics, such as oligosaccharides, are commonly consumed to regulate gut microbiotas in healthy individuals.
28884590	1	2	theme	type	261:264	arg1	diabetes					268:275	type 2 diabetes	261:275	type 2 diabetes	261:275	Gut microbiota affects the host's metabolism, and it is suggested that there are differences in gut microbiota composition between patients with type 2 diabetes and healthy individuals.
28884590	5	3	theme	metabolic	845:853	arg1	endotoxemia					855:865	metabolic endotoxemia	845:865	metabolic endotoxemia	845:865	Recent studies have reported that Bifidobacteriaceae improved metabolic endotoxemia or impaired glucose tolerance.
28884590	7	4	with	patients	1200:1207	arg1	diabetes					1214:1221	diabetes	1214:1221	diabetes	1214:1221	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	6	5	with	patients	961:968	arg1	diabetes					982:989	type 2 diabetes	975:989	type 2 diabetes	975:989	However, there are few studies reporting the effects of GOS on patients with type 2 diabetes.
28884590	12	6	theme	continuous	2002:2011	arg1	method					2046:2051	a promising method	2034:2051	a promising method for managing type 2 diabetes	2034:2080	In the present study, we demonstrated that GOS can ameliorate dysbiosis in patients with diabetes, and continuous intake of GOS may be a promising method for managing type 2 diabetes.
28884590	12	6	theme	continuous	2002:2011	arg1	intake					2013:2018	continuous intake	2002:2018	continuous intake of GOS	2002:2025	In the present study, we demonstrated that GOS can ameliorate dysbiosis in patients with diabetes, and continuous intake of GOS may be a promising method for managing type 2 diabetes.
28884590	7	7	theme	faecal	1047:1052	arg1	microbiota					1058:1067	faecal gut microbiota	1047:1067	faecal gut microbiota	1047:1067	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	5	8	theme	Recent	783:788	arg1	studies					790:796	Recent studies	783:796	Recent studies	783:796	Recent studies have reported that Bifidobacteriaceae improved metabolic endotoxemia or impaired glucose tolerance.
28884590	6	9	theme	type	975:978	arg1	diabetes					982:989	type 2 diabetes	975:989	type 2 diabetes	975:989	However, there are few studies reporting the effects of GOS on patients with type 2 diabetes.
28884590	12	10	theme	type	2066:2069	arg1	diabetes					2073:2080	type 2 diabetes	2066:2080	type 2 diabetes	2066:2080	In the present study, we demonstrated that GOS can ameliorate dysbiosis in patients with diabetes, and continuous intake of GOS may be a promising method for managing type 2 diabetes.
28884590	7	11	theme	clinical	1026:1033	arg1	parameters					1035:1044	clinical parameters	1026:1044	clinical parameters	1026:1044	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	7	11	theme	clinical	1026:1033	arg1	LPS					1135:1137	LPS	1135:1137	LPS	1135:1137	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	10	12	with	patients	1655:1662	arg1	diabetes					1669:1676	diabetes	1669:1676	diabetes	1669:1676	The abundance of Bifidobacteriaceae and the diversity of intestinal microbiota were significantly lower in patients with diabetes than in healthy subjects.
28884590	1	13	with	patients	247:254	arg1	diabetes					268:275	type 2 diabetes	261:275	type 2 diabetes	261:275	Gut microbiota affects the host's metabolism, and it is suggested that there are differences in gut microbiota composition between patients with type 2 diabetes and healthy individuals.
28884590	8	14	from	microbiotas	1311:1321	arg1	patients					1342:1349	patients	1342:1349	patients with type 2 diabetes	1342:1370	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	8	14	from	microbiotas	1311:1321	arg1	control					1294:1300	glycaemic control	1284:1300	glycaemic control	1284:1300	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	8	15	theme	type	1356:1359	arg1	diabetes					1363:1370	type 2 diabetes	1356:1370	type 2 diabetes	1356:1370	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	9	16	theme	healthy	1486:1492	arg1	subjects					1494:1501	healthy subjects	1486:1501	healthy subjects	1486:1501	LBP levels were significantly higher in patients with diabetes than those of healthy subjects, which was consistent with previous reports.
28884590	4	17	theme	Bifidobacteriaceae	753:770	arg1	abundance					772:780	Bifidobacteriaceae abundance	753:780	Bifidobacteriaceae abundance	753:780	Galacto-oligosaccharides (GOS) are a major prebiotic, which specifically increase Bifidobacteriaceae abundance.
28884590	7	18	theme	LPS-binding	1144:1154	arg1	LBP					1165:1167	LBP	1165:1167	LBP	1165:1167	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	7	18	theme	LPS-binding	1144:1154	arg1	protein					1156:1162	LPS-binding protein	1144:1162	LPS-binding protein (LBP) produced by the host	1144:1189	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	8	19	theme	glycaemic	1284:1292	arg1	control					1294:1300	glycaemic control	1284:1300	glycaemic control	1284:1300	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	4	20	theme	major	708:712	arg1	prebiotic					714:722	a major prebiotic	706:722	a major prebiotic	706:722	Galacto-oligosaccharides (GOS) are a major prebiotic, which specifically increase Bifidobacteriaceae abundance.
28884590	4	20	theme	major	708:712	arg1	Galacto-oligosaccharides					671:694	Galacto-oligosaccharides	671:694	Galacto-oligosaccharides (GOS)	671:700	Galacto-oligosaccharides (GOS) are a major prebiotic, which specifically increase Bifidobacteriaceae abundance.
28884590	3	21	theme	Several	460:466	arg1	studies					468:474	Several studies	460:474	Several studies	460:474	Several studies have reported relationships between metabolic diseases and the gut microbiota; and prebiotics, such as oligosaccharides, are commonly consumed to regulate gut microbiotas in healthy individuals.
28884590	5	22	theme	glucose	879:885	arg1	tolerance					887:895	impaired glucose tolerance	870:895	impaired glucose tolerance	870:895	Recent studies have reported that Bifidobacteriaceae improved metabolic endotoxemia or impaired glucose tolerance.
28884590	11	23	theme	glucose	1828:1834	arg1	tolerance					1836:1844	glucose tolerance	1828:1844	glucose tolerance	1828:1844	Interestingly, Bifidobacteriaceae was markedly restored in patients with diabetes after consumption of GOS, whereas LBP and glucose tolerance did not improve during this short-term trial period.
28884590	7	24	theme	current	999:1005	arg1	study					1007:1011	the current study	995:1011	the current study	995:1011	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	6	25	theme	GOS	954:956	arg1	effects					943:949	the effects	939:949	the effects of GOS on patients with type 2 diabetes	939:989	However, there are few studies reporting the effects of GOS on patients with type 2 diabetes.
28884590	12	26	theme	GOS	2023:2025	arg1	method					2046:2051	a promising method	2034:2051	a promising method for managing type 2 diabetes	2034:2080	In the present study, we demonstrated that GOS can ameliorate dysbiosis in patients with diabetes, and continuous intake of GOS may be a promising method for managing type 2 diabetes.
28884590	12	26	theme	GOS	2023:2025	arg1	intake					2013:2018	continuous intake	2002:2018	continuous intake of GOS	2002:2025	In the present study, we demonstrated that GOS can ameliorate dysbiosis in patients with diabetes, and continuous intake of GOS may be a promising method for managing type 2 diabetes.
28884590	8	27	theme	controlled	1390:1399	arg1	manner					1401:1406	a double-blind controlled manner	1375:1406	a double-blind controlled manner	1375:1406	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	2	28	theme	metabolic	395:403	arg1	endotoxemia					405:415	metabolic endotoxemia	395:415	metabolic endotoxemia	395:415	Additionally, dysbiosis may increase the concentration of lipopolysaccharides (LPS), causing metabolic endotoxemia, which induces impaired glucose tolerance.
28884590	1	29	theme	healthy	281:287	arg1	individuals					289:299	healthy individuals	281:299	healthy individuals	281:299	Gut microbiota affects the host's metabolism, and it is suggested that there are differences in gut microbiota composition between patients with type 2 diabetes and healthy individuals.
28884590	9	30	with	consistent	1514:1523	arg1	reports					1539:1545	previous reports	1530:1545	previous reports	1530:1545	LBP levels were significantly higher in patients with diabetes than those of healthy subjects, which was consistent with previous reports.
28884590	1	31	theme	Gut	116:118	arg1	microbiota					120:129	Gut microbiota	116:129	Gut microbiota	116:129	Gut microbiota affects the host's metabolism, and it is suggested that there are differences in gut microbiota composition between patients with type 2 diabetes and healthy individuals.
28884590	8	32	theme	GOS	1277:1279	arg1	effects					1266:1272	the effects	1262:1272	the effects of GOS on glycaemic control	1262:1300	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	8	32	theme	GOS	1277:1279	arg1	metabolites					1327:1337	metabolites	1327:1337	metabolites	1327:1337	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	8	32	theme	GOS	1277:1279	arg1	microbiotas					1311:1321	gut microbiotas	1307:1321	gut microbiotas	1307:1321	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	0	33	theme	Bifidobacteriaceae	46:63	arg1	decline					65:71	dysbiotic Bifidobacteriaceae decline	36:71	dysbiotic Bifidobacteriaceae decline	36:71	Galacto-oligosaccharides ameliorate dysbiotic Bifidobacteriaceae decline in Japanese patients with type 2 diabetes.
28884590	12	34	with	patients	1974:1981	arg1	diabetes					1988:1995	diabetes	1988:1995	diabetes	1988:1995	In the present study, we demonstrated that GOS can ameliorate dysbiosis in patients with diabetes, and continuous intake of GOS may be a promising method for managing type 2 diabetes.
28884590	1	35	with	individuals	289:299	arg1	diabetes					268:275	type 2 diabetes	261:275	type 2 diabetes	261:275	Gut microbiota affects the host's metabolism, and it is suggested that there are differences in gut microbiota composition between patients with type 2 diabetes and healthy individuals.
28884590	8	36	from	metabolites	1327:1337	arg1	patients					1342:1349	patients	1342:1349	patients with type 2 diabetes	1342:1370	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	8	36	from	metabolites	1327:1337	arg1	control					1294:1300	glycaemic control	1284:1300	glycaemic control	1284:1300	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	2	37	theme	glucose	441:447	arg1	tolerance					449:457	impaired glucose tolerance	432:457	impaired glucose tolerance	432:457	Additionally, dysbiosis may increase the concentration of lipopolysaccharides (LPS), causing metabolic endotoxemia, which induces impaired glucose tolerance.
28884590	0	38	theme	dysbiotic	36:44	arg1	decline					65:71	dysbiotic Bifidobacteriaceae decline	36:71	dysbiotic Bifidobacteriaceae decline	36:71	Galacto-oligosaccharides ameliorate dysbiotic Bifidobacteriaceae decline in Japanese patients with type 2 diabetes.
28884590	2	39	theme	impaired	432:439	arg1	tolerance					449:457	impaired glucose tolerance	432:457	impaired glucose tolerance	432:457	Additionally, dysbiosis may increase the concentration of lipopolysaccharides (LPS), causing metabolic endotoxemia, which induces impaired glucose tolerance.
28884590	11	40	with	patients	1763:1770	arg1	diabetes					1777:1784	diabetes	1777:1784	diabetes after consumption of GOS	1777:1809	Interestingly, Bifidobacteriaceae was markedly restored in patients with diabetes after consumption of GOS, whereas LBP and glucose tolerance did not improve during this short-term trial period.
28884590	1	41	theme	gut	212:214	arg1	composition					227:237	gut microbiota composition	212:237	gut microbiota composition	212:237	Gut microbiota affects the host's metabolism, and it is suggested that there are differences in gut microbiota composition between patients with type 2 diabetes and healthy individuals.
28884590	11	42	theme	trial	1885:1889	arg1	period					1891:1896	this short-term trial period	1869:1896	this short-term trial period	1869:1896	Interestingly, Bifidobacteriaceae was markedly restored in patients with diabetes after consumption of GOS, whereas LBP and glucose tolerance did not improve during this short-term trial period.
28884590	12	43	theme	present	1906:1912	arg1	study					1914:1918	the present study	1902:1918	the present study	1902:1918	In the present study, we demonstrated that GOS can ameliorate dysbiosis in patients with diabetes, and continuous intake of GOS may be a promising method for managing type 2 diabetes.
28884590	3	44	theme	gut	631:633	arg1	microbiotas					635:645	gut microbiotas	631:645	gut microbiotas	631:645	Several studies have reported relationships between metabolic diseases and the gut microbiota; and prebiotics, such as oligosaccharides, are commonly consumed to regulate gut microbiotas in healthy individuals.
28884590	9	45	with	patients	1449:1456	arg1	diabetes					1463:1470	diabetes	1463:1470	diabetes	1463:1470	LBP levels were significantly higher in patients with diabetes than those of healthy subjects, which was consistent with previous reports.
28884590	1	46	theme	microbiota	216:225	arg1	composition					227:237	gut microbiota composition	212:237	gut microbiota composition	212:237	Gut microbiota affects the host's metabolism, and it is suggested that there are differences in gut microbiota composition between patients with type 2 diabetes and healthy individuals.
28884590	5	47	theme	impaired	870:877	arg1	tolerance					887:895	impaired glucose tolerance	870:895	impaired glucose tolerance	870:895	Recent studies have reported that Bifidobacteriaceae improved metabolic endotoxemia or impaired glucose tolerance.
28884590	10	48	theme	microbiota	1616:1625	arg1	lower					1646:1650	lower	1646:1650	lower	1646:1650	The abundance of Bifidobacteriaceae and the diversity of intestinal microbiota were significantly lower in patients with diabetes than in healthy subjects.
28884590	10	48	theme	microbiota	1616:1625	arg1	diversity					1592:1600	the diversity	1588:1600	the diversity of intestinal microbiota	1588:1625	The abundance of Bifidobacteriaceae and the diversity of intestinal microbiota were significantly lower in patients with diabetes than in healthy subjects.
28884590	10	48	theme	microbiota	1616:1625	arg1	abundance					1552:1560	The abundance	1548:1560	The abundance of Bifidobacteriaceae	1548:1582	The abundance of Bifidobacteriaceae and the diversity of intestinal microbiota were significantly lower in patients with diabetes than in healthy subjects.
28884590	7	49	with	controls	1235:1242	arg1	diabetes					1214:1221	diabetes	1214:1221	diabetes	1214:1221	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	7	50	theme	associated	1076:1085	arg1	products					1097:1104	associated metabolic products	1076:1104	associated metabolic products	1076:1104	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	7	50	theme	associated	1076:1085	arg1	LPS					1135:1137	LPS	1135:1137	LPS	1135:1137	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	2	51	theme	lipopolysaccharides	360:378	arg1	concentration					343:355	the concentration	339:355	the concentration of lipopolysaccharides (LPS)	339:384	Additionally, dysbiosis may increase the concentration of lipopolysaccharides (LPS), causing metabolic endotoxemia, which induces impaired glucose tolerance.
28884590	0	52	theme	Japanese	76:83	arg1	patients					85:92	Japanese patients	76:92	Japanese patients with type 2 diabetes	76:113	Galacto-oligosaccharides ameliorate dysbiotic Bifidobacteriaceae decline in Japanese patients with type 2 diabetes.
28884590	12	53	from	dysbiosis	1961:1969	arg1	patients					1974:1981	patients	1974:1981	patients with diabetes	1974:1995	In the present study, we demonstrated that GOS can ameliorate dysbiosis in patients with diabetes, and continuous intake of GOS may be a promising method for managing type 2 diabetes.
28884590	7	54	theme	healthy	1227:1233	arg1	controls					1235:1242	healthy controls	1227:1242	healthy controls	1227:1242	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	1	55	from	differences	197:207	arg1	composition					227:237	gut microbiota composition	212:237	gut microbiota composition	212:237	Gut microbiota affects the host's metabolism, and it is suggested that there are differences in gut microbiota composition between patients with type 2 diabetes and healthy individuals.
28884590	8	56	theme	gut	1307:1309	arg1	microbiotas					1311:1321	gut microbiotas	1307:1321	gut microbiotas	1307:1321	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	11	57	theme	short-term	1874:1883	arg1	period					1891:1896	this short-term trial period	1869:1896	this short-term trial period	1869:1896	Interestingly, Bifidobacteriaceae was markedly restored in patients with diabetes after consumption of GOS, whereas LBP and glucose tolerance did not improve during this short-term trial period.
28884590	7	58	theme	metabolic	1087:1095	arg1	products					1097:1104	associated metabolic products	1076:1104	associated metabolic products	1076:1104	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	7	58	theme	metabolic	1087:1095	arg1	LPS					1135:1137	LPS	1135:1137	LPS	1135:1137	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	6	59	theme	few	917:919	arg1	studies					921:927	few studies	917:927	few studies reporting the effects of GOS on patients with type 2 diabetes	917:989	However, there are few studies reporting the effects of GOS on patients with type 2 diabetes.
28884590	3	60	theme	healthy	650:656	arg1	individuals					658:668	healthy individuals	650:668	healthy individuals	650:668	Several studies have reported relationships between metabolic diseases and the gut microbiota; and prebiotics, such as oligosaccharides, are commonly consumed to regulate gut microbiotas in healthy individuals.
28884590	8	61	from	effects	1266:1272	arg1	patients					1342:1349	patients	1342:1349	patients with type 2 diabetes	1342:1370	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	8	61	from	effects	1266:1272	arg1	control					1294:1300	glycaemic control	1284:1300	glycaemic control	1284:1300	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	3	62	theme	metabolic	512:520	arg1	diseases					522:529	metabolic diseases	512:529	metabolic diseases	512:529	Several studies have reported relationships between metabolic diseases and the gut microbiota; and prebiotics, such as oligosaccharides, are commonly consumed to regulate gut microbiotas in healthy individuals.
28884590	9	63	theme	LBP	1409:1411	arg1	levels					1413:1418	LBP levels	1409:1418	LBP levels	1409:1418	LBP levels were significantly higher in patients with diabetes than those of healthy subjects, which was consistent with previous reports.
28884590	8	64	with	patients	1342:1349	arg1	diabetes					1363:1370	type 2 diabetes	1356:1370	type 2 diabetes	1356:1370	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	0	65	with	patients	85:92	arg1	diabetes					106:113	type 2 diabetes	99:113	type 2 diabetes	99:113	Galacto-oligosaccharides ameliorate dysbiotic Bifidobacteriaceae decline in Japanese patients with type 2 diabetes.
28884590	9	66	theme	previous	1530:1537	arg1	reports					1539:1545	previous reports	1530:1545	previous reports	1530:1545	LBP levels were significantly higher in patients with diabetes than those of healthy subjects, which was consistent with previous reports.
28884590	8	67	theme	double-blind	1377:1388	arg1	manner					1401:1406	a double-blind controlled manner	1375:1406	a double-blind controlled manner	1375:1406	We then assessed the effects of GOS on glycaemic control, and gut microbiotas and metabolites in patients with type 2 diabetes in a double-blind controlled manner.
28884590	0	68	theme	type	99:102	arg1	diabetes					106:113	type 2 diabetes	99:113	type 2 diabetes	99:113	Galacto-oligosaccharides ameliorate dysbiotic Bifidobacteriaceae decline in Japanese patients with type 2 diabetes.
28884590	11	69	theme	GOS	1807:1809	arg1	consumption					1792:1802	consumption	1792:1802	consumption of GOS	1792:1809	Interestingly, Bifidobacteriaceae was markedly restored in patients with diabetes after consumption of GOS, whereas LBP and glucose tolerance did not improve during this short-term trial period.
28884590	10	70	theme	healthy	1686:1692	arg1	subjects					1694:1701	healthy subjects	1686:1701	healthy subjects	1686:1701	The abundance of Bifidobacteriaceae and the diversity of intestinal microbiota were significantly lower in patients with diabetes than in healthy subjects.
28884590	7	71	theme	gut	1054:1056	arg1	microbiota					1058:1067	faecal gut microbiota	1047:1067	faecal gut microbiota	1047:1067	In the current study, we compared clinical parameters, faecal gut microbiota, their associated metabolic products and their components such as LPS, and LPS-binding protein (LBP) produced by the host, between patients with diabetes and healthy controls.
28884590	12	72	theme	promising	2036:2044	arg1	method					2046:2051	a promising method	2034:2051	a promising method for managing type 2 diabetes	2034:2080	In the present study, we demonstrated that GOS can ameliorate dysbiosis in patients with diabetes, and continuous intake of GOS may be a promising method for managing type 2 diabetes.
28884590	12	72	theme	promising	2036:2044	arg1	intake					2013:2018	continuous intake	2002:2018	continuous intake of GOS	2002:2025	In the present study, we demonstrated that GOS can ameliorate dysbiosis in patients with diabetes, and continuous intake of GOS may be a promising method for managing type 2 diabetes.
28884590	6	73	from	effects	943:949	arg1	patients					961:968	patients	961:968	patients with type 2 diabetes	961:989	However, there are few studies reporting the effects of GOS on patients with type 2 diabetes.
28884590	10	74	theme	intestinal	1605:1614	arg1	microbiota					1616:1625	intestinal microbiota	1605:1625	intestinal microbiota	1605:1625	The abundance of Bifidobacteriaceae and the diversity of intestinal microbiota were significantly lower in patients with diabetes than in healthy subjects.
24931534	7	0	theme	higher	1681:1686	arg1	NPA					1688:1690	a higher NPA	1679:1690	a higher NPA of TAA with starch versus fiber diets	1679:1728	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	3	1	theme	blood	832:836	arg1	samples					838:844	6 hourly blood samples	823:844	6 hourly blood samples	823:844	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	10	2	from	rich	2313:2316	arg1	starch					2321:2326	starch	2321:2326	starch	2321:2326	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	5	3	theme	β-hydroxybutyrate	1229:1245	arg1	higher					1252:1257	higher	1252:1257	higher	1252:1257	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	5	3	theme	β-hydroxybutyrate	1229:1245	arg1	NPA					1180:1182	NPA	1180:1182	NPA	1180:1182	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	5	3	theme	β-hydroxybutyrate	1229:1245	arg1	appearance					1168:1177	the net portal appearance	1153:1177	the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate	1153:1245	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	3	4	theme	splanchnic	1019:1028	arg1	tissues					1030:1036	total splanchnic tissues	1013:1036	total splanchnic tissues	1013:1036	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	2	5	theme	rich	554:557	arg1	[diets					547:552	2 different carbohydrate compositions [diets	509:552	2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP)	509:783	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	3	6	theme	portal	870:875	arg1	veins					889:893	the portal and hepatic veins	866:893	the portal and hepatic veins as well as the mesenteric artery	866:926	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	10	7	theme	requirements	2439:2450	arg1	result					2416:2421	the result	2412:2421	the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements	2335:2450	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	0	8	from	levels	124:129	arg1	diets					92:96	diets	92:96	diets at low and normal protein levels	92:129	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	9	9	dep	TAA	2173:2175	arg1	NPA					2166:2168	NPA	2166:2168	NPA	2166:2168	After correcting for differences in NPA of TAA, the net hepatic uptake of TAA tended to be lower with starch versus fiber diets.
24931534	2	10	theme	starch	578:583	arg1	350g					570:573	350g	570:573	350g of starch and 310g of neutral detergent fiber/kg of dry matter	570:636	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	7	11	theme	TAA	1773:1775	arg1	AA					1805:1806	essential and nonessential AA	1778:1806	essential and nonessential AA	1778:1806	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	7	11	theme	TAA	1773:1775	arg1	flux					1765:1768	the net hepatic flux	1749:1768	the net hepatic flux of TAA	1749:1775	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	5	12	theme	fatty	1212:1216	arg1	acids					1218:1222	total volatile fatty acids	1197:1222	total volatile fatty acids	1197:1222	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	1	13	theme	multicatheterized	151:167	arg1	cows					176:179	Five mid-lactation multicatheterized Jersey cows	132:179	Five mid-lactation multicatheterized Jersey cows	132:179	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	10	14	theme	higher	2263:2268	arg1	consequence					2339:2349	the consequence	2335:2349	the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements	2335:2450	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	10	14	theme	higher	2263:2268	arg1	transfer					2270:2277	The higher transfer	2259:2277	The higher transfer of N from feed to milk with diets rich in starch	2259:2326	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	6	15	from	fiber	1498:1502	arg1	rich					1490:1493	rich	1490:1493	rich	1490:1493	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	2	16	from	rich	646:649	arg1	fiber					654:658	fiber	654:658	fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP)	654:783	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	16	from	rich	646:649	arg1	rich					554:557	rich	554:557	rich	554:557	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	1	17	theme	crude	430:434	arg1	content					449:455	the dietary crude protein (CP) content	418:455	the dietary crude protein (CP) content	418:455	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	5	18	from	fiber	1278:1282	arg1	rich					1270:1273	rich	1270:1273	rich	1270:1273	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	3	19	theme	hepatic	881:887	arg1	veins					889:893	the portal and hepatic veins	866:893	the portal and hepatic veins as well as the mesenteric artery	866:926	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	2	20	theme	detergent	695:703	arg1	fiber/kg					705:712	neutral detergent fiber/kg	687:712	neutral detergent fiber/kg of dry matter	687:726	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	21	from	fiber	654:658	arg1	rich					646:649	rich	646:649	rich	646:649	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	5	22	from	rich	1270:1273	arg1	starch					1291:1296	starch	1291:1296	starch	1291:1296	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	5	22	from	rich	1270:1273	arg1	fiber					1278:1282	fiber	1278:1282	fiber	1278:1282	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	7	23	theme	N	1914:1914	arg1	yield					1916:1920	a greater (+7%) milk N yield	1893:1920	a greater (+7%) milk N yield	1893:1920	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	2	24	dep	starch	562:567	arg1	350g					570:573	350g	570:573	350g of starch and 310g of neutral detergent fiber/kg of dry matter	570:636	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	8	25	theme	hepatic	1931:1937	arg1	flux					1939:1942	The net hepatic flux	1923:1942	The net hepatic flux	1923:1942	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	25	theme	hepatic	1931:1937	arg1	removal					1967:1973	hepatic fractional removal	1948:1973	hepatic fractional removal of none of the individual AA	1948:2002	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	25	theme	hepatic	1931:1937	arg1	carbohydrate					2029:2040	the main carbohydrate	2020:2040	the main carbohydrate changed from fiber to starch	2020:2069	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	2	26	theme	compositions	534:545	arg1	[diets					547:552	2 different carbohydrate compositions [diets	509:552	2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP)	509:783	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	1	27	used	used	186:189	arg2	cows					176:179	Five mid-lactation multicatheterized Jersey cows	132:179	Five mid-lactation multicatheterized Jersey cows	132:179	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	10	28	from	starch	2321:2326	arg1	rich					2313:2316	rich	2313:2316	rich	2313:2316	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	0	29	theme	protein	116:122	arg1	levels					124:129	low and normal protein levels	101:129	low and normal protein levels	101:129	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	1	30	theme	AA	369:370	arg1	metabolism					372:381	the splanchnic AA metabolism	354:381	the splanchnic AA metabolism	354:381	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	9	31	theme	TAA	2173:2175	arg1	differences					2151:2161	differences	2151:2161	differences in NPA of TAA	2151:2175	After correcting for differences in NPA of TAA, the net hepatic uptake of TAA tended to be lower with starch versus fiber diets.
24931534	5	32	from	starch	1291:1296	arg1	rich					1270:1273	rich	1270:1273	rich	1270:1273	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	2	33	theme	different	511:519	arg1	[diets					547:552	2 different carbohydrate compositions [diets	509:552	2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP)	509:783	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	34	theme	matter	631:636	arg1	fiber/kg					615:622	neutral detergent fiber/kg	597:622	neutral detergent fiber/kg of dry matter	597:636	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	11	35	theme	peripheral	2536:2545	arg1	tissues					2547:2553	peripheral tissues	2536:2553	peripheral tissues with starch versus fiber diets	2536:2584	A better AA use by peripheral tissues with starch versus fiber diets was also hypothesized but more studies are warranted to clarify this issue.
24931534	8	36	theme	fractional	1956:1965	arg1	carbohydrate					2029:2040	the main carbohydrate	2020:2040	the main carbohydrate changed from fiber to starch	2020:2069	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	36	theme	fractional	1956:1965	arg1	removal					1967:1973	hepatic fractional removal	1948:1973	hepatic fractional removal of none of the individual AA	1948:2002	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	36	theme	fractional	1956:1965	arg1	flux					1939:1942	The net hepatic flux	1923:1942	The net hepatic flux	1923:1942	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	7	37	theme	greater	1895:1901	arg1	yield					1916:1920	a greater (+7%) milk N yield	1893:1920	a greater (+7%) milk N yield	1893:1920	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	7	38	theme	yield	1916:1920	arg1	release					1867:1873	a higher (+22%) net splanchnic release	1836:1873	a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield	1836:1920	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	1	39	theme	rich	294:297	arg1	diets					288:292	diets	288:292	diets rich in starch versus fiber	288:320	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	9	40	theme	hepatic	2186:2192	arg1	uptake					2194:2199	the net hepatic uptake	2178:2199	the net hepatic uptake of TAA	2178:2206	After correcting for differences in NPA of TAA, the net hepatic uptake of TAA tended to be lower with starch versus fiber diets.
24931534	9	40	theme	hepatic	2186:2192	arg1	lower					2221:2225	lower	2221:2225	lower	2221:2225	After correcting for differences in NPA of TAA, the net hepatic uptake of TAA tended to be lower with starch versus fiber diets.
24931534	6	41	theme	rich	1490:1493	arg1	diets					1484:1488	diets	1484:1488	diets rich in fiber versus starch (42.3 vs. 51.4%, respectively)	1484:1547	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	6	42	theme	AA	1615:1616	arg1	NPA					1599:1601	the NPA	1595:1601	the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp)	1595:1668	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	8	43	theme	main	2024:2027	arg1	carbohydrate					2029:2040	the main carbohydrate	2020:2040	the main carbohydrate changed from fiber to starch	2020:2069	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	43	theme	main	2024:2027	arg1	removal					1967:1973	hepatic fractional removal	1948:1973	hepatic fractional removal of none of the individual AA	1948:2002	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	43	theme	main	2024:2027	arg1	flux					1939:1942	The net hepatic flux	1923:1942	The net hepatic flux	1923:1942	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	0	44	theme	acids	68:72	arg1	availability					46:57	the posthepatic availability	30:57	the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels	30:129	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	3	45	theme	mesenteric	910:919	arg1	artery					921:926	the mesenteric artery	906:926	the portal and hepatic veins as well as the mesenteric artery	866:926	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	4	46	theme	calculated	1054:1063	arg1	intake					1072:1077	calculated energy intake	1054:1077	calculated energy intake	1054:1077	Dry matter and calculated energy intake as well as total absorbed energy were similar across treatments.
24931534	0	47	theme	dairy	77:81	arg1	cows					83:86	dairy cows	77:86	dairy cows fed diets at low and normal protein levels	77:129	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	6	48	theme	N	1414:1414	arg1	intake					1416:1421	N intake	1414:1421	N intake recovered as total AA (TAA) in the portal vein	1414:1468	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	2	49	dep	fiber	654:658	arg1	45g					661:663	45g	661:663	45g of starch and 460g of neutral detergent fiber/kg of dry matter	661:726	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	8	50	theme	individual	1990:1999	arg1	AA					2001:2002	the individual AA	1986:2002	the individual AA	1986:2002	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	7	51	theme	AA	1878:1879	arg1	release					1867:1873	a higher (+22%) net splanchnic release	1836:1873	a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield	1836:1920	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	5	52	theme	net	1157:1159	arg1	higher					1252:1257	higher	1252:1257	higher	1252:1257	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	5	52	theme	net	1157:1159	arg1	NPA					1180:1182	NPA	1180:1182	NPA	1180:1182	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	5	52	theme	net	1157:1159	arg1	appearance					1168:1177	the net portal appearance	1153:1177	the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate	1153:1245	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	3	53	theme	net	941:943	arg1	fluxes					954:959	net nutrient fluxes	941:959	net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues	941:1036	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	10	54	with	milk	2297:2300	arg1	diets					2307:2311	diets	2307:2311	diets rich in starch	2307:2326	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	10	55	theme	effect	2374:2379	arg1	consequence					2339:2349	the consequence	2335:2349	the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements	2335:2450	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	10	55	theme	effect	2374:2379	arg1	transfer					2270:2277	The higher transfer	2259:2277	The higher transfer of N from feed to milk with diets rich in starch	2259:2326	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	6	56	dep	PDV	1646:1648	arg1	Glu					1651:1653	Glu	1651:1653	Glu	1651:1653	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	6	56	dep	PDV	1646:1648	arg1	Gln					1656:1658	Gln	1656:1658	Gln	1656:1658	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	6	56	dep	PDV	1646:1648	arg1	Asp					1665:1667	Asp	1665:1667	Asp	1665:1667	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	6	57	theme	portal	1458:1463	arg1	vein					1465:1468	the portal vein	1454:1468	the portal vein	1454:1468	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	7	58	theme	higher	1838:1843	arg1	release					1867:1873	a higher (+22%) net splanchnic release	1836:1873	a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield	1836:1920	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	2	59	theme	detergent	605:613	arg1	fiber/kg					615:622	neutral detergent fiber/kg	597:622	neutral detergent fiber/kg of dry matter	597:636	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	1	60	theme	milk	259:262	arg1	yield					266:270	milk N yield	259:270	milk N yield associated with diets rich in starch versus fiber	259:320	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	10	61	theme	diets	2395:2399	arg1	effect					2374:2379	a direct sparing AA effect	2354:2379	a direct sparing AA effect of glucogenic diets	2354:2399	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	11	62	theme	better	2519:2524	arg1	use					2529:2531	A better AA use	2517:2531	A better AA use by peripheral tissues with starch versus fiber diets	2517:2584	A better AA use by peripheral tissues with starch versus fiber diets was also hypothesized but more studies are warranted to clarify this issue.
24931534	2	63	theme	CP	781:782	arg1	contents					756:763	2 different CP contents	741:763	2 different CP contents (12.0 vs. 16.5% CP)	741:783	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	63	theme	CP	781:782	arg1	%					779:779	12.0 vs. 16.5% CP	766:782	%	779:779	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	8	64	theme	none	1978:1981	arg1	flux					1939:1942	The net hepatic flux	1923:1942	The net hepatic flux	1923:1942	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	64	theme	none	1978:1981	arg1	removal					1967:1973	hepatic fractional removal	1948:1973	hepatic fractional removal of none of the individual AA	1948:2002	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	64	theme	none	1978:1981	arg1	carbohydrate					2029:2040	the main carbohydrate	2020:2040	the main carbohydrate changed from fiber to starch	2020:2069	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	10	65	theme	energy	2432:2437	arg1	requirements					2439:2450	lower energy requirements	2426:2450	lower energy requirements	2426:2450	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	10	66	theme	N	2282:2282	arg1	consequence					2339:2349	the consequence	2335:2349	the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements	2335:2450	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	10	66	theme	N	2282:2282	arg1	transfer					2270:2277	The higher transfer	2259:2277	The higher transfer of N from feed to milk with diets rich in starch	2259:2326	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	11	67	with	tissues	2547:2553	arg1	starch					2560:2565	starch	2560:2565	starch	2560:2565	A better AA use by peripheral tissues with starch versus fiber diets was also hypothesized but more studies are warranted to clarify this issue.
24931534	11	67	with	tissues	2547:2553	arg1	diets					2580:2584	fiber diets	2574:2584	fiber diets	2574:2584	A better AA use by peripheral tissues with starch versus fiber diets was also hypothesized but more studies are warranted to clarify this issue.
24931534	1	68	theme	CP	445:446	arg1	content					449:455	the dietary crude protein (CP) content	418:455	the dietary crude protein (CP) content	418:455	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	0	69	theme	posthepatic	34:44	arg1	availability					46:57	the posthepatic availability	30:57	the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels	30:129	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	3	70	theme	treatment	805:813	arg1	period					815:820	each treatment period	800:820	each treatment period	800:820	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	7	71	theme	nonessential	1792:1803	arg1	AA					1805:1806	essential and nonessential AA	1778:1806	essential and nonessential AA	1778:1806	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	7	71	theme	nonessential	1792:1803	arg1	flux					1765:1768	the net hepatic flux	1749:1768	the net hepatic flux of TAA	1749:1775	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	5	72	theme	volatile	1203:1210	arg1	acids					1218:1222	total volatile fatty acids	1197:1222	total volatile fatty acids	1197:1222	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	1	73	theme	4×4	196:198	arg1	design					213:218	a 4×4 Latin square design	194:218	a 4×4 Latin square design	194:218	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	2	74	theme	dry	717:719	arg1	matter					721:726	dry matter	717:726	dry matter	717:726	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	4	75	theme	total	1090:1094	arg1	energy					1105:1110	total absorbed energy	1090:1110	total absorbed energy	1090:1110	Dry matter and calculated energy intake as well as total absorbed energy were similar across treatments.
24931534	7	76	theme	essential	1778:1786	arg1	AA					1805:1806	essential and nonessential AA	1778:1806	essential and nonessential AA	1778:1806	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	7	76	theme	essential	1778:1786	arg1	flux					1765:1768	the net hepatic flux	1749:1768	the net hepatic flux of TAA	1749:1775	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	10	77	theme	sparing	2363:2369	arg1	effect					2374:2379	a direct sparing AA effect	2354:2379	a direct sparing AA effect of glucogenic diets	2354:2399	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	1	78	theme	square	206:211	arg1	design					213:218	a 4×4 Latin square design	194:218	a 4×4 Latin square design	194:218	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	7	79	theme	TAA	1695:1697	arg1	NPA					1688:1690	a higher NPA	1679:1690	a higher NPA of TAA with starch versus fiber diets	1679:1728	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	6	80	located	observed	1583:1590	arg1	NPA					1599:1601	the NPA	1595:1601	the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp)	1595:1668	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	6	80	located	observed	1583:1590	arg2	difference					1572:1581	any difference	1568:1581	any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp)	1568:1668	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	3	81	theme	hourly	825:830	arg1	samples					838:844	6 hourly blood samples	823:844	6 hourly blood samples	823:844	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	2	82	theme	different	743:751	arg1	contents					756:763	2 different CP contents	741:763	2 different CP contents (12.0 vs. 16.5% CP)	741:783	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	82	theme	different	743:751	arg1	%					779:779	12.0 vs. 16.5% CP	766:782	%	779:779	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	7	83	dep	higher	1838:1843	arg1	%					1849:1849	+22%	1846:1849	+22%	1846:1849	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	10	84	theme	higher	2476:2481	arg1	flow					2495:2498	a higher microbial N flow	2474:2498	a higher microbial N flow to the duodenum	2474:2514	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	2	85	theme	16.5	775:778	arg1	contents					756:763	2 different CP contents	741:763	2 different CP contents (12.0 vs. 16.5% CP)	741:783	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	85	theme	16.5	775:778	arg1	%					779:779	12.0 vs. 16.5% CP	766:782	%	779:779	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	1	86	theme	dietary	422:428	arg1	content					449:455	the dietary crude protein (CP) content	418:455	the dietary crude protein (CP) content	418:455	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	2	87	theme	460g	679:682	arg1	45g					661:663	45g	661:663	45g of starch and 460g of neutral detergent fiber/kg of dry matter	661:726	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	88	theme	12.0	766:769	arg1	contents					756:763	2 different CP contents	741:763	2 different CP contents (12.0 vs. 16.5% CP)	741:783	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	88	theme	12.0	766:769	arg1	%					779:779	12.0 vs. 16.5% CP	766:782	%	779:779	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	1	89	theme	mid-lactation	137:149	arg1	cows					176:179	Five mid-lactation multicatheterized Jersey cows	132:179	Five mid-lactation multicatheterized Jersey cows	132:179	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	3	90	theme	total	1013:1017	arg1	tissues					1030:1036	total splanchnic tissues	1013:1036	total splanchnic tissues	1013:1036	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	10	91	from	transfer	2270:2277	arg1	feed					2289:2292	feed	2289:2292	feed	2289:2292	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	2	92	theme	starch	668:673	arg1	45g					661:663	45g	661:663	45g of starch and 460g of neutral detergent fiber/kg of dry matter	661:726	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	1	93	from	increase	247:254	arg1	yield					266:270	milk N yield	259:270	milk N yield associated with diets rich in starch versus fiber	259:320	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	5	94	theme	acids	1218:1222	arg1	higher					1252:1257	higher	1252:1257	higher	1252:1257	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	5	94	theme	acids	1218:1222	arg1	NPA					1180:1182	NPA	1180:1182	NPA	1180:1182	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	5	94	theme	acids	1218:1222	arg1	appearance					1168:1177	the net portal appearance	1153:1177	the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate	1153:1245	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	1	95	theme	Jersey	169:174	arg1	cows					176:179	Five mid-lactation multicatheterized Jersey cows	132:179	Five mid-lactation multicatheterized Jersey cows	132:179	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	10	96	theme	N	2493:2493	arg1	flow					2495:2498	a higher microbial N flow	2474:2498	a higher microbial N flow to the duodenum	2474:2514	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	2	97	theme	fiber/kg	705:712	arg1	460g					679:682	460g	679:682	460g	679:682	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	97	theme	fiber/kg	705:712	arg1	starch					668:673	starch	668:673	starch	668:673	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	0	98	theme	low	101:103	arg1	levels					124:129	low and normal protein levels	101:129	low and normal protein levels	101:129	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	1	99	theme	protein	436:442	arg1	content					449:455	the dietary crude protein (CP) content	418:455	the dietary crude protein (CP) content	418:455	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	4	100	theme	energy	1065:1070	arg1	intake					1072:1077	calculated energy intake	1054:1077	calculated energy intake	1054:1077	Dry matter and calculated energy intake as well as total absorbed energy were similar across treatments.
24931534	8	101	theme	net	1927:1929	arg1	flux					1939:1942	The net hepatic flux	1923:1942	The net hepatic flux	1923:1942	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	101	theme	net	1927:1929	arg1	removal					1967:1973	hepatic fractional removal	1948:1973	hepatic fractional removal of none of the individual AA	1948:2002	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	101	theme	net	1927:1929	arg1	carbohydrate					2029:2040	the main carbohydrate	2020:2040	the main carbohydrate changed from fiber to starch	2020:2069	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	10	102	from	feed	2289:2292	arg1	consequence					2339:2349	the consequence	2335:2349	the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements	2335:2450	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	10	102	from	feed	2289:2292	arg1	N					2282:2282	N	2282:2282	N from feed	2282:2292	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	10	102	from	feed	2289:2292	arg1	transfer					2270:2277	The higher transfer	2259:2277	The higher transfer of N from feed to milk with diets rich in starch	2259:2326	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	7	103	theme	milk	1909:1912	arg1	yield					1916:1920	a greater (+7%) milk N yield	1893:1920	a greater (+7%) milk N yield	1893:1920	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	2	104	theme	neutral	687:693	arg1	fiber/kg					705:712	neutral detergent fiber/kg	687:712	neutral detergent fiber/kg of dry matter	687:726	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	7	105	theme	hepatic	1757:1763	arg1	AA					1805:1806	essential and nonessential AA	1778:1806	essential and nonessential AA	1778:1806	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	7	105	theme	hepatic	1757:1763	arg1	flux					1765:1768	the net hepatic flux	1749:1768	the net hepatic flux of TAA	1749:1775	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	0	106	theme	normal	109:114	arg1	levels					124:129	low and normal protein levels	101:129	low and normal protein levels	101:129	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	1	107	theme	splanchnic	358:367	arg1	metabolism					372:381	the splanchnic AA metabolism	354:381	the splanchnic AA metabolism	354:381	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	8	108	theme	hepatic	1948:1954	arg1	carbohydrate					2029:2040	the main carbohydrate	2020:2040	the main carbohydrate changed from fiber to starch	2020:2069	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	108	theme	hepatic	1948:1954	arg1	removal					1967:1973	hepatic fractional removal	1948:1973	hepatic fractional removal of none of the individual AA	1948:2002	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	8	108	theme	hepatic	1948:1954	arg1	flux					1939:1942	The net hepatic flux	1923:1942	The net hepatic flux	1923:1942	The net hepatic flux and hepatic fractional removal of none of the individual AA was affected as the main carbohydrate changed from fiber to starch, except for Gly and Lys, which were higher for the latter.
24931534	2	109	theme	carbohydrate	521:532	arg1	[diets					547:552	2 different carbohydrate compositions [diets	509:552	2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP)	509:783	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	10	110	theme	microbial	2483:2491	arg1	flow					2495:2498	a higher microbial N flow	2474:2498	a higher microbial N flow to the duodenum	2474:2514	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	6	111	from	starch	1511:1516	arg1	rich					1490:1493	rich	1490:1493	rich	1490:1493	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	6	112	theme	total	1436:1440	arg1	AA					1442:1443	total AA	1436:1443	total AA (TAA)	1436:1449	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	6	112	theme	total	1436:1440	arg1	TAA					1446:1448	TAA	1446:1448	TAA	1446:1448	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	10	113	theme	rich	2313:2316	arg1	diets					2307:2311	diets	2307:2311	diets rich in starch	2307:2326	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	6	114	theme	energy	1626:1631	arg1	fuels					1633:1637	energy fuels	1626:1637	energy fuels	1626:1637	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	0	115	theme	amino	62:66	arg1	acids					68:72	amino acids	62:72	amino acids	62:72	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	11	116	theme	fiber	2574:2578	arg1	diets					2580:2584	fiber diets	2574:2584	fiber diets	2574:2584	A better AA use by peripheral tissues with starch versus fiber diets was also hypothesized but more studies are warranted to clarify this issue.
24931534	7	117	theme	net	1753:1755	arg1	AA					1805:1806	essential and nonessential AA	1778:1806	essential and nonessential AA	1778:1806	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	7	117	theme	net	1753:1755	arg1	flux					1765:1768	the net hepatic flux	1749:1768	the net hepatic flux of TAA	1749:1775	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	9	118	theme	net	2182:2184	arg1	uptake					2194:2199	the net hepatic uptake	2178:2199	the net hepatic uptake of TAA	2178:2206	After correcting for differences in NPA of TAA, the net hepatic uptake of TAA tended to be lower with starch versus fiber diets.
24931534	9	118	theme	net	2182:2184	arg1	lower					2221:2225	lower	2221:2225	lower	2221:2225	After correcting for differences in NPA of TAA, the net hepatic uptake of TAA tended to be lower with starch versus fiber diets.
24931534	6	119	dep	lower	1474:1478	arg1	Concomitant					1366:1376	Concomitant	1366:1376	Concomitant	1366:1376	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	6	119	dep	lower	1474:1478	arg1	difference					1572:1581	any difference	1568:1581	any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp)	1568:1668	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	6	119	dep	lower	1474:1478	arg1	without					1550:1556	without	1550:1556	without	1550:1556	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	0	120	from	starch	14:19	arg1	rich					6:9	rich	6:9	rich	6:9	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	7	121	theme	fiber	1718:1722	arg1	diets					1724:1728	fiber diets	1718:1728	fiber diets	1718:1728	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	10	122	theme	direct	2356:2361	arg1	effect					2374:2379	a direct sparing AA effect	2354:2379	a direct sparing AA effect of glucogenic diets	2354:2399	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	6	123	from	rich	1490:1493	arg1	starch					1511:1516	starch	1511:1516	starch	1511:1516	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	6	123	from	rich	1490:1493	arg1	fiber					1498:1502	fiber	1498:1502	fiber	1498:1502	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	0	124	from	rich	6:9	arg1	starch					14:19	starch	14:19	starch	14:19	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	2	125	theme	neutral	597:603	arg1	fiber/kg					615:622	neutral detergent fiber/kg	597:622	neutral detergent fiber/kg of dry matter	597:636	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	6	126	theme	main	1610:1613	arg1	AA					1615:1616	the main AA	1606:1616	the main AA used as energy fuels by the PDV (Glu, Gln, and Asp)	1606:1668	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	7	127	dep	greater	1895:1901	arg1	%					1906:1906	+7%	1904:1906	+7%	1904:1906	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	6	128	theme	intake	1416:1421	arg1	percentage					1400:1409	the percentage	1396:1409	the percentage of N intake recovered as total AA (TAA) in the portal vein	1396:1468	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	6	128	theme	intake	1416:1421	arg1	lower					1474:1478	lower	1474:1478	lower	1474:1478	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	9	129	theme	TAA	2204:2206	arg1	uptake					2194:2199	the net hepatic uptake	2178:2199	the net hepatic uptake of TAA	2178:2206	After correcting for differences in NPA of TAA, the net hepatic uptake of TAA tended to be lower with starch versus fiber diets.
24931534	9	129	theme	TAA	2204:2206	arg1	lower					2221:2225	lower	2221:2225	lower	2221:2225	After correcting for differences in NPA of TAA, the net hepatic uptake of TAA tended to be lower with starch versus fiber diets.
24931534	2	130	theme	isoenergetic	463:474	arg1	diets					476:480	Four isoenergetic diets	458:480	Four isoenergetic diets	458:480	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	0	131	from	availability	46:57	arg1	cows					83:86	dairy cows	77:86	dairy cows fed diets at low and normal protein levels	77:129	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	2	132	theme	310g	589:592	arg1	350g					570:573	350g	570:573	350g of starch and 310g of neutral detergent fiber/kg of dry matter	570:636	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	7	133	theme	net	1852:1854	arg1	release					1867:1873	a higher (+22%) net splanchnic release	1836:1873	a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield	1836:1920	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	1	134	from	changes	343:349	arg1	metabolism					372:381	the splanchnic AA metabolism	354:381	the splanchnic AA metabolism	354:381	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	10	135	theme	glucogenic	2384:2393	arg1	diets					2395:2399	glucogenic diets	2384:2399	glucogenic diets	2384:2399	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	7	136	theme	splanchnic	1856:1865	arg1	release					1867:1873	a higher (+22%) net splanchnic release	1836:1873	a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield	1836:1920	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	5	137	theme	rich	1270:1273	arg1	diets					1264:1268	diets	1264:1268	diets rich in fiber versus starch	1264:1296	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	2	138	theme	dry	627:629	arg1	matter					631:636	dry matter	627:636	dry matter	627:636	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	5	139	theme	portal	1161:1166	arg1	higher					1252:1257	higher	1252:1257	higher	1252:1257	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	5	139	theme	portal	1161:1166	arg1	NPA					1180:1182	NPA	1180:1182	NPA	1180:1182	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	5	139	theme	portal	1161:1166	arg1	appearance					1168:1177	the net portal appearance	1153:1177	the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate	1153:1245	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	0	140	theme	rich	6:9	arg1	Diets					0:4	Diets	0:4	Diets rich in starch	0:19	Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels.
24931534	10	141	theme	AA	2371:2372	arg1	effect					2374:2379	a direct sparing AA effect	2354:2379	a direct sparing AA effect of glucogenic diets	2354:2399	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	2	142	from	rich	554:557	arg1	starch					562:567	starch	562:567	starch	562:567	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	142	from	rich	554:557	arg1	rich					646:649	rich	646:649	rich	646:649	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	143	theme	fiber/kg	615:622	arg1	310g					589:592	310g	589:592	310g	589:592	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	143	theme	fiber/kg	615:622	arg1	starch					578:583	starch	578:583	starch	578:583	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	1	144	from	fiber	316:320	arg1	rich					294:297	rich	294:297	rich	294:297	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	7	145	from	differences	1734:1744	arg1	AA					1805:1806	essential and nonessential AA	1778:1806	essential and nonessential AA	1778:1806	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	7	145	from	differences	1734:1744	arg1	flux					1765:1768	the net hepatic flux	1749:1768	the net hepatic flux of TAA	1749:1775	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	2	146	from	starch	562:567	arg1	rich					554:557	rich	554:557	rich	554:557	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	11	147	theme	more	2612:2615	arg1	studies					2617:2623	more studies	2612:2623	more studies	2612:2623	A better AA use by peripheral tissues with starch versus fiber diets was also hypothesized but more studies are warranted to clarify this issue.
24931534	1	148	from	starch	302:307	arg1	rich					294:297	rich	294:297	rich	294:297	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	1	149	theme	N	264:264	arg1	yield					266:270	milk N yield	259:270	milk N yield associated with diets rich in starch versus fiber	259:320	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	3	150	theme	nutrient	945:952	arg1	fluxes					954:959	net nutrient fluxes	941:959	net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues	941:1036	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	6	151	dep	fiber	1498:1502	arg1	%					1532:1532	42.3 vs. 51.4%	1519:1532	42.3 vs. 51.4%	1519:1532	Concomitant to these changes, the percentage of N intake recovered as total AA (TAA) in the portal vein was lower for diets rich in fiber versus starch (42.3 vs. 51.4%, respectively), without, however, any difference observed in the NPA of the main AA used as energy fuels by the PDV (Glu, Gln, and Asp).
24931534	9	152	theme	fiber	2246:2250	arg1	diets					2252:2256	fiber diets	2246:2256	fiber diets	2246:2256	After correcting for differences in NPA of TAA, the net hepatic uptake of TAA tended to be lower with starch versus fiber diets.
24931534	5	153	theme	acetate	1188:1194	arg1	higher					1252:1257	higher	1252:1257	higher	1252:1257	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	5	153	theme	acetate	1188:1194	arg1	NPA					1180:1182	NPA	1180:1182	NPA	1180:1182	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	5	153	theme	acetate	1188:1194	arg1	appearance					1168:1177	the net portal appearance	1153:1177	the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate	1153:1245	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	1	154	from	rich	294:297	arg1	starch					302:307	starch	302:307	starch	302:307	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	1	154	from	rich	294:297	arg1	fiber					316:320	fiber	316:320	fiber	316:320	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	11	155	theme	AA	2526:2527	arg1	use					2529:2531	A better AA use	2517:2531	A better AA use by peripheral tissues with starch versus fiber diets	2517:2584	A better AA use by peripheral tissues with starch versus fiber diets was also hypothesized but more studies are warranted to clarify this issue.
24931534	10	156	theme	lower	2426:2430	arg1	requirements					2439:2450	lower energy requirements	2426:2450	lower energy requirements	2426:2450	The higher transfer of N from feed to milk with diets rich in starch is not the consequence of a direct sparing AA effect of glucogenic diets but rather the result of lower energy requirements by the PDV along with a higher microbial N flow to the duodenum.
24931534	5	157	theme	total	1197:1201	arg1	acids					1218:1222	total volatile fatty acids	1197:1222	total volatile fatty acids	1197:1222	However, the net portal appearance (NPA) of acetate, total volatile fatty acids, and β-hydroxybutyrate were higher with diets rich in fiber versus starch, whereas that of oxygen, glucose, butyrate, and insulin were lower.
24931534	3	158	theme	portal-drained	972:985	arg1	viscera					987:993	the portal-drained viscera	968:993	the portal-drained viscera (PDV)	968:999	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	3	158	theme	portal-drained	972:985	arg1	PDV					996:998	PDV	996:998	PDV	996:998	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	4	159	theme	Dry	1039:1041	arg1	matter					1043:1048	Dry matter	1039:1048	Dry matter	1039:1048	Dry matter and calculated energy intake as well as total absorbed energy were similar across treatments.
24931534	3	160	theme	period	815:820	arg1	end					793:795	the end	789:795	the end of each treatment period	789:820	At the end of each treatment period, 6 hourly blood samples were collected from the portal and hepatic veins as well as the mesenteric artery to determine net nutrient fluxes across the portal-drained viscera (PDV), liver, and total splanchnic tissues.
24931534	2	161	theme	matter	721:726	arg1	fiber/kg					705:712	neutral detergent fiber/kg	687:712	neutral detergent fiber/kg of dry matter	687:726	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	4	162	theme	absorbed	1096:1103	arg1	energy					1105:1110	total absorbed energy	1090:1110	total absorbed energy	1090:1110	Dry matter and calculated energy intake as well as total absorbed energy were similar across treatments.
24931534	7	163	with	NPA	1688:1690	arg1	starch					1704:1709	starch	1704:1709	starch	1704:1709	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	7	163	with	NPA	1688:1690	arg1	diets					1724:1728	fiber diets	1718:1728	fiber diets	1718:1728	Despite a higher NPA of TAA with starch versus fiber diets, no differences in the net hepatic flux of TAA, essential and nonessential AA were observed, resulting in a higher (+22%) net splanchnic release of AA and, hence, a greater (+7%) milk N yield.
24931534	1	164	theme	Latin	200:204	arg1	design					213:218	a 4×4 Latin square design	194:218	a 4×4 Latin square design	194:218	Five mid-lactation multicatheterized Jersey cows were used in a 4×4 Latin square design to investigate whether the increase in milk N yield associated with diets rich in starch versus fiber could originate from changes in the splanchnic AA metabolism and if these changes depended upon the dietary crude protein (CP) content.
24931534	2	165	theme	CP	753:754	arg1	contents					756:763	2 different CP contents	741:763	2 different CP contents (12.0 vs. 16.5% CP)	741:783	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
24931534	2	165	theme	CP	753:754	arg1	%					779:779	12.0 vs. 16.5% CP	766:782	%	779:779	Four isoenergetic diets were formulated to provide 2 different carbohydrate compositions [diets rich in starch (350g of starch and 310g of neutral detergent fiber/kg of dry matter) versus rich in fiber (45g of starch and 460g of neutral detergent fiber/kg of dry matter)] crossed by 2 different CP contents (12.0 vs. 16.5% CP).
27255605	7	0	theme	%	1241:1241	arg1	contents					1221:1228	GAG and collagen contents	1204:1228	GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively	1204:1292	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	7	0	theme	%	1241:1241	arg1	EY=344±113kPa					1186:1198	EY=344±113kPa	1186:1198	EY=344±113kPa	1186:1198	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	3	1	from	properties	488:497	arg1	constructs					502:511	constructs	502:511	constructs this large	502:522	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	8	2	with	Constructs	1295:1304	arg1	channels					1314:1321	27 channels	1311:1321	27 channels	1311:1321	Constructs with 27 channels had significantly less deposited GAG than other groups.
27255605	8	3	contain	had	1323:1325	arg2	GAG					1356:1358	significantly less deposited GAG	1327:1358	significantly less deposited GAG	1327:1358	Constructs with 27 channels had significantly less deposited GAG than other groups.
27255605	8	3	contain	had	1323:1325	arg1	Constructs					1295:1304	Constructs	1295:1304	Constructs with 27 channels	1295:1321	Constructs with 27 channels had significantly less deposited GAG than other groups.
27255605	0	4	theme	cartilage	80:88	arg1	constructs					90:99	large engineered cartilage constructs	63:99	large engineered cartilage constructs	63:99	Optimizing nutrient channel spacing and revisiting TGF-beta in large engineered cartilage constructs.
27255605	4	5	theme	Young׳s	626:632	arg1	EY					643:644	EY	643:644	EY	643:644	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	4	5	theme	Young׳s	626:632	arg1	modulus					634:640	native compressive Young׳s modulus	607:640	native compressive Young׳s modulus (EY)	607:645	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	7	6	theme	construct	1259:1267	arg1	weight					1273:1278	construct wet weight	1259:1278	construct wet weight	1259:1278	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	6	7	theme	10ng/mL	1024:1030	arg1	TGF-β					1032:1036	10ng/mL TGF-β	1024:1036	10ng/mL TGF-β	1024:1036	We cultured ∅10mm constructs with 0, 12, 19, or 27 nutrient channels (∅1mm) for 6-8 weeks with 0, 1, or 10ng/mL TGF-β; subsequently we analyzed them mechanically, biochemically, and histologically.
27255605	4	8	theme	compressive	614:624	arg1	EY					643:644	EY	643:644	EY	643:644	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	4	8	theme	compressive	614:624	arg1	modulus					634:640	native compressive Young׳s modulus	607:640	native compressive Young׳s modulus (EY)	607:645	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	8	9	theme	other	1365:1369	arg1	groups					1371:1376	other groups	1365:1376	other groups	1365:1376	Constructs with 27 channels had significantly less deposited GAG than other groups.
27255605	5	10	theme	channel	842:848	arg1	spacing					850:856	channel spacing	842:856	channel spacing	842:856	To ultimately produce articular layer-sized constructs, we must first optimize channel spacing and investigate the role of TGF-β in the utility of channels.
27255605	4	11	theme	TGF-β	567:571	arg1	treatment					573:581	Transient active TGF-β treatment	550:581	Transient active TGF-β treatment	550:581	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	10	12	with	constructs	1470:1479	arg1	channels					1494:1501	0 or 12 channels	1486:1501	0 or 12 channels	1486:1501	Without TGF-β, constructs with 0 or 12 channels exhibited properties that were indistinguishable, and lower than TGF-β-supplemented constructs.
27255605	3	13	theme	channel	409:415	arg1	spacings					417:424	optimal channel spacings	401:424	optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large	401:522	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	0	14	from	TGF-beta	51:58	arg1	constructs					90:99	large engineered cartilage constructs	63:99	large engineered cartilage constructs	63:99	Optimizing nutrient channel spacing and revisiting TGF-beta in large engineered cartilage constructs.
27255605	11	15	theme	TGF-β	1700:1704	arg1	presence					1688:1695	the presence	1684:1695	the presence of TGF-β	1684:1704	Taken together, these results emphasize that nutrient channels are effective only in the presence of TGF-β, and indicate that spacings equivalent to 12 channels in ∅10mm constructs can be employed in articular-layer-sized constructs with reduced dosages of TGF-β.
27255605	4	16	theme	active	560:565	arg1	treatment					573:581	Transient active TGF-β treatment	550:581	Transient active TGF-β treatment	550:581	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	11	17	from	channels	1751:1758	arg1	constructs					1769:1778	∅10mm constructs	1763:1778	∅10mm constructs	1763:1778	Taken together, these results emphasize that nutrient channels are effective only in the presence of TGF-β, and indicate that spacings equivalent to 12 channels in ∅10mm constructs can be employed in articular-layer-sized constructs with reduced dosages of TGF-β.
27255605	11	18	theme	articular-layer-sized	1799:1819	arg1	constructs					1821:1830	articular-layer-sized constructs	1799:1830	articular-layer-sized constructs with reduced dosages of TGF-β	1799:1860	Taken together, these results emphasize that nutrient channels are effective only in the presence of TGF-β, and indicate that spacings equivalent to 12 channels in ∅10mm constructs can be employed in articular-layer-sized constructs with reduced dosages of TGF-β.
27255605	3	19	from	compositional	459:471	arg1	constructs					502:511	constructs	502:511	constructs this large	502:522	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	9	20	theme	similar	1435:1441	arg1	properties					1443:1452	similar properties	1435:1452	similar properties	1435:1452	Channeled constructs given 1 or 10ng/mL TGF-β developed similar properties.
27255605	11	21	from	presence	1688:1695	arg1	effective					1666:1674	effective	1666:1674	effective	1666:1674	Taken together, these results emphasize that nutrient channels are effective only in the presence of TGF-β, and indicate that spacings equivalent to 12 channels in ∅10mm constructs can be employed in articular-layer-sized constructs with reduced dosages of TGF-β.
27255605	4	22	from	content	675:681	arg1	constructs					686:695	constructs	686:695	constructs	686:695	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	0	23	theme	nutrient	11:18	arg1	spacing					28:34	nutrient channel spacing	11:34	nutrient channel spacing	11:34	Optimizing nutrient channel spacing and revisiting TGF-beta in large engineered cartilage constructs.
27255605	3	24	dep	spacings	417:424	arg1	recapitulate					429:440	recapitulate	429:440	to recapitulate native cartilage compositional and mechanical properties in constructs this large	426:522	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	11	25	from	effective	1666:1674	arg1	presence					1688:1695	the presence	1684:1695	the presence of TGF-β	1684:1704	Taken together, these results emphasize that nutrient channels are effective only in the presence of TGF-β, and indicate that spacings equivalent to 12 channels in ∅10mm constructs can be employed in articular-layer-sized constructs with reduced dosages of TGF-β.
27255605	5	26	theme	channels	910:917	arg1	utility					899:905	the utility	895:905	the utility of channels	895:917	To ultimately produce articular layer-sized constructs, we must first optimize channel spacing and investigate the role of TGF-β in the utility of channels.
27255605	4	27	theme	glycosaminoglycan	651:667	arg1	content					675:681	glycosaminoglycan (GAG) content	651:681	glycosaminoglycan (GAG) content in constructs	651:695	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	3	28	theme	close-packed	271:282	arg1	channels					284:291	Increasingly close-packed channels	258:291	Increasingly close-packed channels	258:291	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	1	29	theme	Cartilage	102:110	arg1	approach					146:153	a promising approach	134:153	a promising approach to treat osteoarthritis	134:177	Cartilage tissue engineering is a promising approach to treat osteoarthritis.
27255605	1	29	theme	Cartilage	102:110	arg1	engineering					119:129	Cartilage tissue engineering	102:129	Cartilage tissue engineering	102:129	Cartilage tissue engineering is a promising approach to treat osteoarthritis.
27255605	3	30	dep	constructs	502:511	arg1	large					518:522	large	518:522	large	518:522	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	3	31	theme	native	442:447	arg1	compositional					459:471	native cartilage compositional	442:471	native cartilage compositional	442:471	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	11	32	theme	TGF-β	1856:1860	arg1	dosages					1845:1851	reduced dosages	1837:1851	reduced dosages of TGF-β	1837:1860	Taken together, these results emphasize that nutrient channels are effective only in the presence of TGF-β, and indicate that spacings equivalent to 12 channels in ∅10mm constructs can be employed in articular-layer-sized constructs with reduced dosages of TGF-β.
27255605	3	33	theme	nutrient	314:321	arg1	limitations					333:343	nutrient transport limitations	314:343	nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs	314:394	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	1	34	theme	tissue	112:117	arg1	approach					146:153	a promising approach	134:153	a promising approach to treat osteoarthritis	134:177	Cartilage tissue engineering is a promising approach to treat osteoarthritis.
27255605	1	34	theme	tissue	112:117	arg1	engineering					119:129	Cartilage tissue engineering	102:129	Cartilage tissue engineering	102:129	Cartilage tissue engineering is a promising approach to treat osteoarthritis.
27255605	4	35	theme	10ng/mL	722:728	arg1	dosages					711:717	standard dosages	702:717	standard dosages of 10ng/mL	702:728	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	3	36	theme	cartilage	449:457	arg1	compositional					459:471	native cartilage compositional	442:471	native cartilage compositional	442:471	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	11	37	theme	reduced	1837:1843	arg1	dosages					1845:1851	reduced dosages	1837:1851	reduced dosages of TGF-β	1837:1860	Taken together, these results emphasize that nutrient channels are effective only in the presence of TGF-β, and indicate that spacings equivalent to 12 channels in ∅10mm constructs can be employed in articular-layer-sized constructs with reduced dosages of TGF-β.
27255605	3	38	theme	transport	323:331	arg1	limitations					333:343	nutrient transport limitations	314:343	nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs	314:394	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	11	39	theme	equivalent	1734:1743	arg1	spacings					1725:1732	spacings	1725:1732	spacings equivalent to 12 channels in ∅10mm constructs	1725:1778	Taken together, these results emphasize that nutrient channels are effective only in the presence of TGF-β, and indicate that spacings equivalent to 12 channels in ∅10mm constructs can be employed in articular-layer-sized constructs with reduced dosages of TGF-β.
27255605	8	40	theme	deposited	1346:1354	arg1	GAG					1356:1358	significantly less deposited GAG	1327:1358	significantly less deposited GAG	1327:1358	Constructs with 27 channels had significantly less deposited GAG than other groups.
27255605	10	41	theme	TGF-β-supplemented	1568:1585	arg1	constructs					1587:1596	TGF-β-supplemented constructs	1568:1596	TGF-β-supplemented constructs	1568:1596	Without TGF-β, constructs with 0 or 12 channels exhibited properties that were indistinguishable, and lower than TGF-β-supplemented constructs.
27255605	5	42	theme	TGF-β	886:890	arg1	role					878:881	the role	874:881	the role of TGF-β in the utility of channels	874:917	To ultimately produce articular layer-sized constructs, we must first optimize channel spacing and investigate the role of TGF-β in the utility of channels.
27255605	7	43	theme	collagen	1212:1219	arg1	contents					1221:1228	GAG and collagen contents	1204:1228	GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively	1204:1292	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	2	44	theme	current	189:195	arg1	techniques					197:206	current techniques	189:206	current techniques	189:206	However, current techniques produce tissues too small for clinical relevance.
27255605	4	45	theme	matrix	741:746	arg1	heterogeneity					748:760	matrix heterogeneity	741:760	matrix heterogeneity	741:760	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	9	46	theme	Channeled	1379:1387	arg1	constructs					1389:1398	Channeled constructs	1379:1398	Channeled constructs given 1 or 10ng/mL TGF-β	1379:1423	Channeled constructs given 1 or 10ng/mL TGF-β developed similar properties.
27255605	4	47	theme	Transient	550:558	arg1	treatment					573:581	Transient active TGF-β treatment	550:581	Transient active TGF-β treatment	550:581	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	6	48	with	constructs	938:947	arg1	channels					980:987	0, 12, 19, or 27 nutrient channels	954:987	0, 12, 19, or 27 nutrient channels (∅1mm)	954:994	We cultured ∅10mm constructs with 0, 12, 19, or 27 nutrient channels (∅1mm) for 6-8 weeks with 0, 1, or 10ng/mL TGF-β; subsequently we analyzed them mechanically, biochemically, and histologically.
27255605	6	48	with	constructs	938:947	arg1	∅1mm					990:993	∅1mm	990:993	∅1mm	990:993	We cultured ∅10mm constructs with 0, 12, 19, or 27 nutrient channels (∅1mm) for 6-8 weeks with 0, 1, or 10ng/mL TGF-β; subsequently we analyzed them mechanically, biochemically, and histologically.
27255605	3	49	theme	mechanical	477:486	arg1	properties					488:497	mechanical properties	477:497	mechanical properties	477:497	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	3	50	theme	centimeter-sized	348:363	arg1	constructs					385:394	centimeter-sized chondrocyte-agarose constructs	348:394	centimeter-sized chondrocyte-agarose constructs	348:394	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	0	51	theme	large	63:67	arg1	constructs					90:99	large engineered cartilage constructs	63:99	large engineered cartilage constructs	63:99	Optimizing nutrient channel spacing and revisiting TGF-beta in large engineered cartilage constructs.
27255605	2	52	theme	clinical	238:245	arg1	relevance					247:255	clinical relevance	238:255	clinical relevance	238:255	However, current techniques produce tissues too small for clinical relevance.
27255605	11	53	theme	nutrient	1644:1651	arg1	channels					1653:1660	nutrient channels	1644:1660	nutrient channels	1644:1660	Taken together, these results emphasize that nutrient channels are effective only in the presence of TGF-β, and indicate that spacings equivalent to 12 channels in ∅10mm constructs can be employed in articular-layer-sized constructs with reduced dosages of TGF-β.
27255605	7	54	with	Constructs	1118:1127	arg1	channels					1143:1150	12 or 19 channels	1134:1150	12 or 19 channels	1134:1150	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	4	55	from	modulus	634:640	arg1	constructs					686:695	constructs	686:695	constructs	686:695	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	5	56	from	role	878:881	arg1	utility					899:905	the utility	895:905	the utility of channels	895:917	To ultimately produce articular layer-sized constructs, we must first optimize channel spacing and investigate the role of TGF-β in the utility of channels.
27255605	3	57	theme	chondrocyte-agarose	365:383	arg1	constructs					385:394	centimeter-sized chondrocyte-agarose constructs	348:394	centimeter-sized chondrocyte-agarose constructs	348:394	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	2	58	theme	small	228:232	arg1	tissues					216:222	tissues	216:222	tissues too small for clinical relevance	216:255	However, current techniques produce tissues too small for clinical relevance.
27255605	11	59	with	constructs	1821:1830	arg1	dosages					1845:1851	reduced dosages	1837:1851	reduced dosages of TGF-β	1837:1860	Taken together, these results emphasize that nutrient channels are effective only in the presence of TGF-β, and indicate that spacings equivalent to 12 channels in ∅10mm constructs can be employed in articular-layer-sized constructs with reduced dosages of TGF-β.
27255605	5	60	theme	layer-sized	795:805	arg1	constructs					807:816	articular layer-sized constructs	785:816	articular layer-sized constructs	785:816	To ultimately produce articular layer-sized constructs, we must first optimize channel spacing and investigate the role of TGF-β in the utility of channels.
27255605	11	61	theme	∅10mm	1763:1767	arg1	constructs					1769:1778	∅10mm constructs	1763:1778	∅10mm constructs	1763:1778	Taken together, these results emphasize that nutrient channels are effective only in the presence of TGF-β, and indicate that spacings equivalent to 12 channels in ∅10mm constructs can be employed in articular-layer-sized constructs with reduced dosages of TGF-β.
27255605	1	62	theme	promising	136:144	arg1	approach					146:153	a promising approach	134:153	a promising approach to treat osteoarthritis	134:177	Cartilage tissue engineering is a promising approach to treat osteoarthritis.
27255605	1	62	theme	promising	136:144	arg1	engineering					119:129	Cartilage tissue engineering	102:129	Cartilage tissue engineering	102:129	Cartilage tissue engineering is a promising approach to treat osteoarthritis.
27255605	3	63	from	limitations	333:343	arg1	constructs					385:394	centimeter-sized chondrocyte-agarose constructs	348:394	centimeter-sized chondrocyte-agarose constructs	348:394	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	6	64	theme	∅10mm	932:936	arg1	constructs					938:947	∅10mm constructs	932:947	∅10mm constructs with 0, 12, 19, or 27 nutrient channels (∅1mm)	932:994	We cultured ∅10mm constructs with 0, 12, 19, or 27 nutrient channels (∅1mm) for 6-8 weeks with 0, 1, or 10ng/mL TGF-β; subsequently we analyzed them mechanically, biochemically, and histologically.
27255605	7	65	theme	weight	1273:1278	arg1	weight					1273:1278	construct wet weight	1259:1278	construct wet weight	1259:1278	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	7	65	theme	weight	1273:1278	arg1	%					1241:1241	10.8±1.2%	1233:1241	10.8±1.2%	1233:1241	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	7	65	theme	weight	1273:1278	arg1	%					1254:1254	2.2±0.2%	1247:1254	2.2±0.2% of construct wet weight	1247:1278	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	0	66	theme	engineered	69:78	arg1	constructs					90:99	large engineered cartilage constructs	63:99	large engineered cartilage constructs	63:99	Optimizing nutrient channel spacing and revisiting TGF-beta in large engineered cartilage constructs.
27255605	7	67	theme	wet	1269:1271	arg1	weight					1273:1278	construct wet weight	1259:1278	construct wet weight	1259:1278	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	7	68	theme	%	1254:1254	arg1	contents					1221:1228	GAG and collagen contents	1204:1228	GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively	1204:1292	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	7	68	theme	%	1254:1254	arg1	EY=344±113kPa					1186:1198	EY=344±113kPa	1186:1198	EY=344±113kPa	1186:1198	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	6	69	theme	nutrient	971:978	arg1	channels					980:987	0, 12, 19, or 27 nutrient channels	954:987	0, 12, 19, or 27 nutrient channels (∅1mm)	954:994	We cultured ∅10mm constructs with 0, 12, 19, or 27 nutrient channels (∅1mm) for 6-8 weeks with 0, 1, or 10ng/mL TGF-β; subsequently we analyzed them mechanically, biochemically, and histologically.
27255605	6	69	theme	nutrient	971:978	arg1	∅1mm					990:993	∅1mm	990:993	∅1mm	990:993	We cultured ∅10mm constructs with 0, 12, 19, or 27 nutrient channels (∅1mm) for 6-8 weeks with 0, 1, or 10ng/mL TGF-β; subsequently we analyzed them mechanically, biochemically, and histologically.
27255605	3	70	theme	optimal	401:407	arg1	spacings					417:424	optimal channel spacings	401:424	optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large	401:522	Increasingly close-packed channels have helped overcome nutrient transport limitations in centimeter-sized chondrocyte-agarose constructs, yet optimal channel spacings to recapitulate native cartilage compositional and mechanical properties in constructs this large have not been identified.
27255605	4	71	theme	native	607:612	arg1	EY					643:644	EY	643:644	EY	643:644	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	4	71	theme	native	607:612	arg1	modulus					634:640	native compressive Young׳s modulus	607:640	native compressive Young׳s modulus (EY)	607:645	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
27255605	0	72	theme	channel	20:26	arg1	spacing					28:34	nutrient channel spacing	11:34	nutrient channel spacing	11:34	Optimizing nutrient channel spacing and revisiting TGF-beta in large engineered cartilage constructs.
27255605	7	73	theme	GAG	1204:1206	arg1	contents					1221:1228	GAG and collagen contents	1204:1228	GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively	1204:1292	Constructs with 12 or 19 channels grew the most favorably, reaching EY=344±113kPa and GAG and collagen contents of 10.8±1.2% and 2.2±0.2% of construct wet weight, respectively.
27255605	5	74	theme	articular	785:793	arg1	constructs					807:816	articular layer-sized constructs	785:816	articular layer-sized constructs	785:816	To ultimately produce articular layer-sized constructs, we must first optimize channel spacing and investigate the role of TGF-β in the utility of channels.
27255605	4	75	theme	standard	702:709	arg1	dosages					711:717	standard dosages	702:717	standard dosages of 10ng/mL	702:728	Transient active TGF-β treatment consistently reproduces native compressive Young׳s modulus (EY) and glycosaminoglycan (GAG) content in constructs, but standard dosages of 10ng/mL exacerbate matrix heterogeneity.
29965108	1	0	theme	phosphorus	353:362	arg1	denitrification					238:252	denitrification	238:252	denitrification	238:252	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	0	theme	phosphorus	353:362	arg1	removal					269:275	phosphorus removal	258:275	phosphorus removal	258:275	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	2	1	theme	average	817:823	arg1	rate					833:836	the average removal rate	813:836	the average removal rate of TP	813:842	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	1	2	theme	removal	364:370	arg1	system					382:387	removal composite system	364:387	removal composite system	364:387	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	0	3	from	Community	96:104	arg1	SCSC-S/Fe					109:117	SCSC-S/Fe	109:117	SCSC-S/Fe	109:117	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe].
29965108	1	4	theme	phosphorus	258:267	arg1	removal					269:275	phosphorus removal	258:275	phosphorus removal	258:275	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	0	5	from	[Effects	0:7	arg1	Characteristics					31:45	the Characteristics	27:45	the Characteristics of Nitrogen and Phosphorus Removal	27:80	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe].
29965108	0	5	from	[Effects	0:7	arg1	Community					96:104	Microbial Community	86:104	Microbial Community in SCSC-S/Fe	86:117	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe].
29965108	1	6	from	effect	149:154	arg1	bacteria					198:205	cellulose-degrading bacteria	178:205	cellulose-degrading bacteria	178:205	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	6	from	effect	149:154	arg1	bacteria					224:231	denitrifying bacteria	211:231	denitrifying bacteria	211:231	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	0	7	theme	Microbial	86:94	arg1	Community					96:104	Microbial Community	86:104	Microbial Community in SCSC-S/Fe	86:117	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe].
29965108	2	8	theme	spherical	961:969	arg1	microorganisms					986:999	spherical and rod-shaped microorganisms	961:999	spherical and rod-shaped microorganisms	961:999	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	1	9	dep	nitrogen	340:347	arg1	system					382:387	removal composite system	364:387	removal composite system	364:387	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	10	theme	surface	477:483	arg1	structure					485:493	the surface structure	473:493	the surface structure	473:493	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	11	theme	scanning	574:581	arg1	SEM					604:606	SEM	604:606	SEM	604:606	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	11	theme	scanning	574:581	arg1	microscope					592:601	scanning electron microscope	574:601	scanning electron microscope (SEM)	574:607	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	0	12	from	Characteristics	31:45	arg1	SCSC-S/Fe					109:117	SCSC-S/Fe	109:117	SCSC-S/Fe	109:117	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe].
29965108	2	13	dep	%	810:810	arg1	to					802:803	to	802:803	to	802:803	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	1	14	theme	electron	583:590	arg1	SEM					604:606	SEM	604:606	SEM	604:606	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	14	theme	electron	583:590	arg1	microscope					592:601	scanning electron microscope	574:601	scanning electron microscope (SEM)	574:607	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	15	theme	temperature	159:169	arg1	effect					149:154	the effect	145:154	the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions	145:466	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	2	16	theme	microbial	876:884	arg1	properties					886:895	microbial properties	876:895	microbial properties	876:895	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	2	17	theme	bacteria	1119:1126	arg1	proportion					1092:1101	the proportion	1088:1101	the proportion of denitrifying bacteria	1088:1126	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	2	18	theme	removal	753:759	arg1	15					719:720	15	719:720	15	719:720	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	2	18	theme	removal	753:759	arg1	rate					761:764	the average TN removal rate	738:764	the average TN removal rate of the system	738:778	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	3	19	theme	cellulose	1173:1181	arg1	bacteria					1194:1201	the cellulose -degrading bacteria	1169:1201	the cellulose -degrading bacteria	1169:1201	It can be seen that the cellulose -degrading bacteria were more sensitive to the temperature than the denitrification bacteria, and more obviously affected by the temperature.
29965108	1	20	theme	solid	280:284	arg1	source					293:298	solid carbon source	280:298	solid carbon source	280:298	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	21	theme	microbial	499:507	arg1	properties					509:518	microbial properties	499:518	microbial properties	499:518	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	2	22	theme	denitrifying	1106:1117	arg1	bacteria					1119:1126	denitrifying bacteria	1106:1126	denitrifying bacteria	1106:1126	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	1	23	theme	carbon	286:291	arg1	source					293:298	solid carbon source	280:298	solid carbon source	280:298	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	24	theme	cellulose-degrading	178:196	arg1	bacteria					198:205	cellulose-degrading bacteria	178:205	cellulose-degrading bacteria	178:205	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	0	25	theme	Temperature	12:22	arg1	[Effects					0:7	[Effects	0:7	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe	0:117	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe].
29965108	1	26	theme	source	293:298	arg1	denitrification					238:252	denitrification	238:252	denitrification	238:252	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	26	theme	source	293:298	arg1	removal					269:275	phosphorus removal	258:275	phosphorus removal	258:275	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	2	27	dep	30℃	733:735	arg1	to					730:731	to	730:731	to	730:731	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	3	28	theme	denitrification	1251:1265	arg1	bacteria					1267:1274	the denitrification bacteria	1247:1274	the denitrification bacteria	1247:1274	It can be seen that the cellulose -degrading bacteria were more sensitive to the temperature than the denitrification bacteria, and more obviously affected by the temperature.
29965108	1	29	dep	denitrification	238:252	arg1	the					234:236	the	234:236	the	234:236	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	0	30	dep	Nitrogen	50:57	arg1	Removal					74:80	Removal	74:80	Removal	74:80	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe].
29965108	2	31	theme	system	773:778	arg1	15					719:720	15	719:720	15	719:720	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	2	31	theme	system	773:778	arg1	rate					761:764	the average TN removal rate	738:764	the average TN removal rate of the system	738:778	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	1	32	theme	cellulose	303:311	arg1	nitrogen					340:347	cellulose corncob+sulfur/sponge iron nitrogen	303:347	cellulose corncob+sulfur/sponge iron nitrogen	303:347	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	2	33	theme	%	864:864	arg1	%					874:874	82.58% to 89.15%	859:874	82.58% to 89.15%	859:874	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	2	34	theme	rod-shaped	975:984	arg1	microorganisms					986:999	spherical and rod-shaped microorganisms	961:999	spherical and rod-shaped microorganisms	961:999	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	1	35	theme	denitrifying	211:222	arg1	bacteria					224:231	denitrifying bacteria	211:231	denitrifying bacteria	211:231	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	0	36	theme	Nitrogen	50:57	arg1	Characteristics					31:45	the Characteristics	27:45	the Characteristics of Nitrogen and Phosphorus Removal	27:80	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe].
29965108	0	36	theme	Nitrogen	50:57	arg1	Community					96:104	Microbial Community	86:104	Microbial Community in SCSC-S/Fe	86:117	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe].
29965108	1	37	theme	corncob+sulfur/sponge	313:333	arg1	nitrogen					340:347	cellulose corncob+sulfur/sponge iron nitrogen	303:347	cellulose corncob+sulfur/sponge iron nitrogen	303:347	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	38	theme	corncob	523:529	arg1	structure					485:493	the surface structure	473:493	the surface structure	473:493	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	38	theme	corncob	523:529	arg1	properties					509:518	microbial properties	499:518	microbial properties	499:518	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	39	theme	composite	372:380	arg1	system					382:387	removal composite system	364:387	removal composite system	364:387	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	40	theme	iron	335:338	arg1	nitrogen					340:347	cellulose corncob+sulfur/sponge iron nitrogen	303:347	cellulose corncob+sulfur/sponge iron nitrogen	303:347	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	2	41	theme	cellulose-degrading	1020:1038	arg1	bacteria					1040:1047	cellulose-degrading bacteria	1020:1047	cellulose-degrading bacteria	1020:1047	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	2	42	theme	bacteria	1040:1047	arg1	proportion					1006:1015	the proportion	1002:1015	the proportion of cellulose-degrading bacteria	1002:1047	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	2	42	theme	bacteria	1040:1047	arg1	higher					1060:1065	higher	1060:1065	higher	1060:1065	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	1	43	theme	MiSeq	613:617	arg1	technologies					646:657	MiSeq high-throughput sequencing technologies	613:657	MiSeq high-throughput sequencing technologies	613:657	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	44	theme	nitrogen	340:347	arg1	denitrification					238:252	denitrification	238:252	denitrification	238:252	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	44	theme	nitrogen	340:347	arg1	removal					269:275	phosphorus removal	258:275	phosphorus removal	258:275	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	45	theme	different	435:443	arg1	conditions					457:466	different temperature conditions	435:466	different temperature conditions	435:466	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	46	theme	high-throughput	619:633	arg1	technologies					646:657	MiSeq high-throughput sequencing technologies	613:657	MiSeq high-throughput sequencing technologies	613:657	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	47	theme	temperature	445:455	arg1	conditions					457:466	different temperature conditions	435:466	different temperature conditions	435:466	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	2	48	theme	TN	750:751	arg1	15					719:720	15	719:720	15	719:720	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	2	48	theme	TN	750:751	arg1	rate					761:764	the average TN removal rate	738:764	the average TN removal rate of the system	738:778	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	1	49	theme	sequencing	635:644	arg1	technologies					646:657	MiSeq high-throughput sequencing technologies	613:657	MiSeq high-throughput sequencing technologies	613:657	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	2	50	theme	TP	841:842	arg1	rate					833:836	the average removal rate	813:836	the average removal rate of TP	813:842	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	0	51	theme	Phosphorus	63:72	arg1	Characteristics					31:45	the Characteristics	27:45	the Characteristics of Nitrogen and Phosphorus Removal	27:80	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe].
29965108	0	51	theme	Phosphorus	63:72	arg1	Community					96:104	Microbial Community	86:104	Microbial Community in SCSC-S/Fe	86:117	[Effects of Temperature on the Characteristics of Nitrogen and Phosphorus Removal and Microbial Community in SCSC-S/Fe].
29965108	2	52	theme	average	742:748	arg1	15					719:720	15	719:720	15	719:720	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	2	52	theme	average	742:748	arg1	rate					761:764	the average TN removal rate	738:764	the average TN removal rate of the system	738:778	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	2	53	theme	%	800:800	arg1	%					810:810	78.88% to 92.70%	795:810	78.88% to 92.70%	795:810	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	3	54	theme	-degrading	1183:1192	arg1	bacteria					1194:1201	the cellulose -degrading bacteria	1169:1201	the cellulose -degrading bacteria	1169:1201	It can be seen that the cellulose -degrading bacteria were more sensitive to the temperature than the denitrification bacteria, and more obviously affected by the temperature.
29965108	2	55	dep	%	874:874	arg1	to					866:867	to	866:867	to	866:867	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	1	56	dep	bacteria	198:205	arg1	the					174:176	the	174:176	the	174:176	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	1	56	dep	bacteria	198:205	arg1	analyzed					420:427	analyzed	420:427	was analyzed under different temperature conditions	416:466	In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies.
29965108	2	57	theme	surface	913:919	arg1	reaction					921:928	the surface reaction	909:928	the surface reaction after corncob	909:942	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
29965108	2	58	theme	removal	825:831	arg1	rate					833:836	the average removal rate	813:836	the average removal rate of TP	813:842	The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%.
26046262	10	0	theme	dry	1030:1032	arg1	conditions					1034:1043	dry conditions	1030:1043	dry conditions	1030:1043	In dry conditions, the Young's modulus of cross-linked samples was slightly improved.
26046262	2	1	from	candidates	315:324	arg1	engineering					341:351	bone tissue engineering	329:351	bone tissue engineering	329:351	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	4	2	dep	first	501:505	arg1	mixed					557:561	mixed	557:561	then mixed with CaP matrix	552:577	For this purpose, CS was the first cross-linked with tripolyphosphate (TPP) and then mixed with CaP matrix.
26046262	4	2	dep	first	501:505	arg1	cross-linked					507:518	cross-linked	507:518	cross-linked with tripolyphosphate (TPP)	507:546	For this purpose, CS was the first cross-linked with tripolyphosphate (TPP) and then mixed with CaP matrix.
26046262	4	3	theme	CaP	568:570	arg1	matrix					572:577	CaP matrix	568:577	CaP matrix	568:577	For this purpose, CS was the first cross-linked with tripolyphosphate (TPP) and then mixed with CaP matrix.
26046262	3	4	theme	present	361:367	arg1	study					369:373	the present study	357:373	the present study	357:373	In the present study, some key features of CaP-CS were significantly improved by developing a novel CaP-CS composite.
26046262	11	5	theme	presented	1126:1134	arg1	results					1136:1142	the presented results	1122:1142	the presented results	1122:1142	Based on the presented results, cross-linking does not have a significant effect on porosity.
26046262	0	6	theme	Cross-linked	0:11	arg1	chitosan					13:20	Cross-linked chitosan	0:20	Cross-linked chitosan	0:20	Cross-linked chitosan improves the mechanical properties of calcium phosphate-chitosan cement.
26046262	8	7	theme	cross-linked	810:821	arg1	samples					849:855	Both cross-linked and uncross-linked CaP-CS samples	805:855	Both cross-linked and uncross-linked CaP-CS samples	805:855	Both cross-linked and uncross-linked CaP-CS samples showed excellent washout resistance.
26046262	0	8	link	Cross-linked	0:11	arg1	chitosan					13:20	Cross-linked chitosan	0:20	Cross-linked chitosan	0:20	Cross-linked chitosan improves the mechanical properties of calcium phosphate-chitosan cement.
26046262	5	9	theme	CaP-CS	591:596	arg1	samples					598:604	CaP-CS samples	591:604	CaP-CS samples	591:604	A group of CaP-CS samples without cross-linking was also prepared.
26046262	1	10	theme	Calcium	95:101	arg1	CaP					114:116	CaP	114:116	CaP	114:116	Calcium phosphate (CaP) cements are highly applicable and valuable materials for filling bone defects by minimally invasive procedures.
26046262	1	10	theme	Calcium	95:101	arg1	phosphate					103:111	Calcium phosphate	95:111	Calcium phosphate (CaP)	95:117	Calcium phosphate (CaP) cements are highly applicable and valuable materials for filling bone defects by minimally invasive procedures.
26046262	8	11	theme	uncross-linked	827:840	arg1	samples					849:855	Both cross-linked and uncross-linked CaP-CS samples	805:855	Both cross-linked and uncross-linked CaP-CS samples	805:855	Both cross-linked and uncross-linked CaP-CS samples showed excellent washout resistance.
26046262	1	12	theme	bone	184:187	arg1	defects					189:195	bone defects	184:195	bone defects	184:195	Calcium phosphate (CaP) cements are highly applicable and valuable materials for filling bone defects by minimally invasive procedures.
26046262	13	13	theme	mechanical	1333:1342	arg1	properties					1344:1353	mechanical properties	1333:1353	mechanical properties	1333:1353	However, in the most cases, mechanical properties were enhanced.
26046262	5	14	theme	samples	598:604	arg1	samples					598:604	CaP-CS samples	591:604	CaP-CS samples	591:604	A group of CaP-CS samples without cross-linking was also prepared.
26046262	5	14	theme	samples	598:604	arg1	group					582:586	A group	580:586	A group of CaP-CS samples without cross-linking	580:626	A group of CaP-CS samples without cross-linking was also prepared.
26046262	14	15	theme	CaP-CS	1453:1458	arg1	properties					1439:1448	the mechanical properties	1424:1448	the mechanical properties of CaP-CS	1424:1458	The results have shown that cross-linking can improve the mechanical properties of CaP-CS and hence it can be used for bone tissue engineering applications.
26046262	2	16	theme	candidates	315:324	arg1	one					282:284	one	282:284	one	282:284	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	2	16	theme	candidates	315:324	arg1	biopolymer					249:258	The chitosan (CS) biopolymer	231:258	The chitosan (CS) biopolymer	231:258	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	2	16	theme	candidates	315:324	arg1	candidates					315:324	the promising biomaterial candidates	289:324	the promising biomaterial candidates in bone tissue engineering	289:351	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	3	17	theme	key	381:383	arg1	features					385:392	some key features	376:392	some key features of CaP-CS	376:402	In the present study, some key features of CaP-CS were significantly improved by developing a novel CaP-CS composite.
26046262	10	18	theme	samples	1082:1088	arg1	modulus					1058:1064	the Young's modulus	1046:1064	the Young's modulus of cross-linked samples	1046:1088	In dry conditions, the Young's modulus of cross-linked samples was slightly improved.
26046262	2	19	theme	biomaterial	303:313	arg1	candidates					315:324	the promising biomaterial candidates	289:324	the promising biomaterial candidates in bone tissue engineering	289:351	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	12	20	theme	P/L	1238:1240	arg1	ratio					1242:1246	the P/L ratio	1234:1246	the P/L ratio of a sample	1234:1258	As expected, by increasing the P/L ratio of a sample, ductility and injectability were decreased.
26046262	9	21	theme	Young	940:944	arg1	modulus					948:954	Young's modulus	940:954	Young's modulus	940:954	The most significant effects were observed on Young's modulus and compressive strength in wet condition as well as surface hardness.
26046262	7	22	theme	liquid	764:769	arg1	ratios					775:780	liquid (L) ratios	764:780	liquid (L) ratios	764:780	Additionally, the effect of different powder (P) to liquid (L) ratios was also investigated.
26046262	8	23	link	uncross-linked	827:840	arg1	samples					849:855	Both cross-linked and uncross-linked CaP-CS samples	805:855	Both cross-linked and uncross-linked CaP-CS samples	805:855	Both cross-linked and uncross-linked CaP-CS samples showed excellent washout resistance.
26046262	2	24	theme	promising	293:301	arg1	candidates					315:324	the promising biomaterial candidates	289:324	the promising biomaterial candidates in bone tissue engineering	289:351	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	8	25	theme	CaP-CS	842:847	arg1	samples					849:855	Both cross-linked and uncross-linked CaP-CS samples	805:855	Both cross-linked and uncross-linked CaP-CS samples	805:855	Both cross-linked and uncross-linked CaP-CS samples showed excellent washout resistance.
26046262	3	26	theme	CaP-CS	454:459	arg1	composite					461:469	a novel CaP-CS composite	446:469	a novel CaP-CS composite	446:469	In the present study, some key features of CaP-CS were significantly improved by developing a novel CaP-CS composite.
26046262	13	27	theme	most	1321:1324	arg1	cases					1326:1330	the most cases	1317:1330	the most cases	1317:1330	However, in the most cases, mechanical properties were enhanced.
26046262	11	28	theme	significant	1175:1185	arg1	effect					1187:1192	a significant effect	1173:1192	a significant effect	1173:1192	Based on the presented results, cross-linking does not have a significant effect on porosity.
26046262	8	29	theme	excellent	864:872	arg1	resistance					882:891	excellent washout resistance	864:891	excellent washout resistance	864:891	Both cross-linked and uncross-linked CaP-CS samples showed excellent washout resistance.
26046262	1	30	theme	invasive	210:217	arg1	procedures					219:228	minimally invasive procedures	200:228	minimally invasive procedures	200:228	Calcium phosphate (CaP) cements are highly applicable and valuable materials for filling bone defects by minimally invasive procedures.
26046262	3	31	theme	CaP-CS	397:402	arg1	features					385:392	some key features	376:392	some key features of CaP-CS	376:402	In the present study, some key features of CaP-CS were significantly improved by developing a novel CaP-CS composite.
26046262	8	32	link	cross-linked	810:821	arg1	samples					849:855	Both cross-linked and uncross-linked CaP-CS samples	805:855	Both cross-linked and uncross-linked CaP-CS samples	805:855	Both cross-linked and uncross-linked CaP-CS samples showed excellent washout resistance.
26046262	9	33	theme	surface	1009:1015	arg1	hardness					1017:1024	surface hardness	1009:1024	wet condition as well as surface hardness	984:1024	The most significant effects were observed on Young's modulus and compressive strength in wet condition as well as surface hardness.
26046262	14	34	used	used	1480:1483	arg2	it					1470:1471	it	1470:1471	it	1470:1471	The results have shown that cross-linking can improve the mechanical properties of CaP-CS and hence it can be used for bone tissue engineering applications.
26046262	0	35	theme	mechanical	35:44	arg1	properties					46:55	the mechanical properties	31:55	the mechanical properties of calcium phosphate-chitosan cement	31:92	Cross-linked chitosan improves the mechanical properties of calcium phosphate-chitosan cement.
26046262	7	36	theme	powder	750:755	arg1	effect					730:735	the effect	726:735	the effect of different powder (P) to liquid (L) ratios	726:780	Additionally, the effect of different powder (P) to liquid (L) ratios was also investigated.
26046262	9	37	located	observed	928:935	arg1	condition					988:996	wet condition	984:996	wet condition as well as surface hardness	984:1024	The most significant effects were observed on Young's modulus and compressive strength in wet condition as well as surface hardness.
26046262	9	37	located	observed	928:935	arg2	effects					915:921	The most significant effects	894:921	The most significant effects	894:921	The most significant effects were observed on Young's modulus and compressive strength in wet condition as well as surface hardness.
26046262	9	37	located	observed	928:935	arg1	hardness					1017:1024	surface hardness	1009:1024	wet condition as well as surface hardness	984:1024	The most significant effects were observed on Young's modulus and compressive strength in wet condition as well as surface hardness.
26046262	3	38	theme	novel	448:452	arg1	composite					461:469	a novel CaP-CS composite	446:469	a novel CaP-CS composite	446:469	In the present study, some key features of CaP-CS were significantly improved by developing a novel CaP-CS composite.
26046262	0	39	theme	calcium	60:66	arg1	cement					87:92	calcium phosphate-chitosan cement	60:92	calcium phosphate-chitosan cement	60:92	Cross-linked chitosan improves the mechanical properties of calcium phosphate-chitosan cement.
26046262	10	40	theme	cross-linked	1069:1080	arg1	samples					1082:1088	cross-linked samples	1069:1088	cross-linked samples	1069:1088	In dry conditions, the Young's modulus of cross-linked samples was slightly improved.
26046262	2	41	theme	chitosan	235:242	arg1	one					282:284	one	282:284	one	282:284	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	2	41	theme	chitosan	235:242	arg1	biopolymer					249:258	The chitosan (CS) biopolymer	231:258	The chitosan (CS) biopolymer	231:258	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	2	41	theme	chitosan	235:242	arg1	CS					245:246	CS	245:246	CS	245:246	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	2	41	theme	chitosan	235:242	arg1	candidates					315:324	the promising biomaterial candidates	289:324	the promising biomaterial candidates in bone tissue engineering	289:351	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	8	42	theme	washout	874:880	arg1	resistance					882:891	excellent washout resistance	864:891	excellent washout resistance	864:891	Both cross-linked and uncross-linked CaP-CS samples showed excellent washout resistance.
26046262	7	43	dep	liquid	764:769	arg1	L					772:772	L	772:772	L	772:772	Additionally, the effect of different powder (P) to liquid (L) ratios was also investigated.
26046262	2	44	theme	tissue	334:339	arg1	engineering					341:351	bone tissue engineering	329:351	bone tissue engineering	329:351	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	14	45	theme	mechanical	1428:1437	arg1	properties					1439:1448	the mechanical properties	1424:1448	the mechanical properties of CaP-CS	1424:1458	The results have shown that cross-linking can improve the mechanical properties of CaP-CS and hence it can be used for bone tissue engineering applications.
26046262	7	46	theme	different	740:748	arg1	powder					750:755	different powder	740:755	different powder (P)	740:759	Additionally, the effect of different powder (P) to liquid (L) ratios was also investigated.
26046262	7	46	theme	different	740:748	arg1	P					758:758	P	758:758	P	758:758	Additionally, the effect of different powder (P) to liquid (L) ratios was also investigated.
26046262	14	47	theme	engineering	1501:1511	arg1	applications					1513:1524	bone tissue engineering applications	1489:1524	bone tissue engineering applications	1489:1524	The results have shown that cross-linking can improve the mechanical properties of CaP-CS and hence it can be used for bone tissue engineering applications.
26046262	2	48	theme	bone	329:332	arg1	engineering					341:351	bone tissue engineering	329:351	bone tissue engineering	329:351	The chitosan (CS) biopolymer is also considered as one of the promising biomaterial candidates in bone tissue engineering.
26046262	9	49	theme	wet	984:986	arg1	condition					988:996	wet condition	984:996	wet condition as well as surface hardness	984:1024	The most significant effects were observed on Young's modulus and compressive strength in wet condition as well as surface hardness.
26046262	10	50	link	cross-linked	1069:1080	arg1	samples					1082:1088	cross-linked samples	1069:1088	cross-linked samples	1069:1088	In dry conditions, the Young's modulus of cross-linked samples was slightly improved.
26046262	14	51	theme	tissue	1494:1499	arg1	applications					1513:1524	bone tissue engineering applications	1489:1524	bone tissue engineering applications	1489:1524	The results have shown that cross-linking can improve the mechanical properties of CaP-CS and hence it can be used for bone tissue engineering applications.
26046262	1	52	theme	applicable	138:147	arg1	materials					162:170	highly applicable and valuable materials	131:170	highly applicable and valuable materials for filling bone defects by minimally invasive procedures	131:228	Calcium phosphate (CaP) cements are highly applicable and valuable materials for filling bone defects by minimally invasive procedures.
26046262	0	53	theme	cement	87:92	arg1	properties					46:55	the mechanical properties	31:55	the mechanical properties of calcium phosphate-chitosan cement	31:92	Cross-linked chitosan improves the mechanical properties of calcium phosphate-chitosan cement.
26046262	14	54	theme	bone	1489:1492	arg1	applications					1513:1524	bone tissue engineering applications	1489:1524	bone tissue engineering applications	1489:1524	The results have shown that cross-linking can improve the mechanical properties of CaP-CS and hence it can be used for bone tissue engineering applications.
26046262	11	55	contain	have	1168:1171	arg1	cross-linking					1145:1157	cross-linking	1145:1157	cross-linking	1145:1157	Based on the presented results, cross-linking does not have a significant effect on porosity.
26046262	11	55	contain	have	1168:1171	arg2	effect					1187:1192	a significant effect	1173:1192	a significant effect	1173:1192	Based on the presented results, cross-linking does not have a significant effect on porosity.
26046262	9	56	theme	compressive	960:970	arg1	strength					972:979	compressive strength	960:979	compressive strength	960:979	The most significant effects were observed on Young's modulus and compressive strength in wet condition as well as surface hardness.
26046262	0	57	theme	phosphate-chitosan	68:85	arg1	cement					87:92	calcium phosphate-chitosan cement	60:92	calcium phosphate-chitosan cement	60:92	Cross-linked chitosan improves the mechanical properties of calcium phosphate-chitosan cement.
26046262	12	58	theme	sample	1253:1258	arg1	ratio					1242:1246	the P/L ratio	1234:1246	the P/L ratio of a sample	1234:1258	As expected, by increasing the P/L ratio of a sample, ductility and injectability were decreased.
26046262	6	59	theme	known	695:699	arg1	standards					701:709	the known standards	691:709	the known standards	691:709	Samples were fabricated and tested based on the known standards.
26046262	1	60	theme	valuable	153:160	arg1	materials					162:170	highly applicable and valuable materials	131:170	highly applicable and valuable materials for filling bone defects by minimally invasive procedures	131:228	Calcium phosphate (CaP) cements are highly applicable and valuable materials for filling bone defects by minimally invasive procedures.
26046262	9	61	theme	significant	903:913	arg1	effects					915:921	The most significant effects	894:921	The most significant effects	894:921	The most significant effects were observed on Young's modulus and compressive strength in wet condition as well as surface hardness.
24008178	12	0	theme	Biofilm	1481:1487	arg1	growth					1489:1494	Biofilm growth	1481:1494	Biofilm growth with sucrose pulsing	1481:1515	Biofilm growth with sucrose pulsing also caused preferential degradation of the composite-dentin interface, depending on the composite/adhesive system used.
24008178	11	1	theme	sucrose-pulsed	1339:1352	arg1	biofilms					1354:1361	sucrose-pulsed biofilms	1339:1361	sucrose-pulsed biofilms	1339:1361	Dentin demineralization was greater under sucrose-pulsed biofilms, as the pH dropped <5.5 during pulsing, with LS and Z100 specimens suffering similar degrees of surface mineral loss.
24008178	14	2	theme	dentin	1800:1805	arg1	coverage					1807:1814	the inferior dentin coverage	1787:1814	the inferior dentin coverage	1787:1814	This was attributed to the inferior dentin coverage by Z100 adhesive, which possibly led to a higher level of chemical and enzymatic degradation.
24008178	2	3	theme	interface	323:331	arg1	integrity					289:297	the mechanical integrity	274:297	the mechanical integrity of the dentin-composite interface challenged with multi-species oral biofilms	274:375	This study investigates the mechanical integrity of the dentin-composite interface challenged with multi-species oral biofilms.
24008178	10	4	theme	electron	1184:1191	arg1	spectroscopy					1228:1239	scanning electron microscopy/energy-dispersive X-ray spectroscopy	1175:1239	scanning electron microscopy/energy-dispersive X-ray spectroscopy	1175:1239	Fracture surfaces were examined using scanning electron microscopy/energy-dispersive X-ray spectroscopy to assess demineralization and interfacial degradation.
24008178	11	5	theme	similar	1440:1446	arg1	degrees					1448:1454	similar degrees	1440:1454	similar degrees of surface mineral loss	1440:1478	Dentin demineralization was greater under sucrose-pulsed biofilms, as the pH dropped <5.5 during pulsing, with LS and Z100 specimens suffering similar degrees of surface mineral loss.
24008178	13	6	theme	bond	1682:1685	arg1	reduction					1696:1704	greater bond strength reduction	1674:1704	greater bond strength reduction	1674:1704	Specifically, Z100 specimens showed greater bond strength reduction and more frequent cohesive failure in the adhesive layer.
24008178	1	7	theme	marginal	198:205	arg1	integrity					207:215	marginal integrity	198:215	marginal integrity	198:215	Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries.
24008178	2	8	theme	oral	363:366	arg1	biofilms					368:375	multi-species oral biofilms	349:375	multi-species oral biofilms	349:375	This study investigates the mechanical integrity of the dentin-composite interface challenged with multi-species oral biofilms.
24008178	7	9	with	run	899:901	arg1	pulsing					916:922	sucrose pulsing	908:922	sucrose pulsing	908:922	A sterile saliva-only control group was run with sucrose pulsing.
24008178	4	10	theme	Filtek	669:674	arg1	LS					680:681	Filtek(TM) LS	669:681	Filtek(TM) LS (3M ESPE)	669:691	Dentin-composite disks were made using bovine incisor roots filled with Z100(TM) or Filtek(TM) LS (3M ESPE).
24008178	4	10	theme	Filtek	669:674	arg1	ESPE					687:690	3M ESPE	684:690	3M ESPE	684:690	Dentin-composite disks were made using bovine incisor roots filled with Z100(TM) or Filtek(TM) LS (3M ESPE).
24008178	13	11	theme	Z100	1652:1655	arg1	specimens					1657:1665	Z100 specimens	1652:1665	Z100 specimens	1652:1665	Specifically, Z100 specimens showed greater bond strength reduction and more frequent cohesive failure in the adhesive layer.
24008178	10	12	theme	X-ray	1222:1226	arg1	spectroscopy					1228:1239	scanning electron microscopy/energy-dispersive X-ray spectroscopy	1175:1239	scanning electron microscopy/energy-dispersive X-ray spectroscopy	1175:1239	Fracture surfaces were examined using scanning electron microscopy/energy-dispersive X-ray spectroscopy to assess demineralization and interfacial degradation.
24008178	1	13	theme	resin-based	144:154	arg1	restorations					166:177	dental resin-based composite restorations	137:177	dental resin-based composite restorations	137:177	Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries.
24008178	3	14	theme	most	384:387	arg1	studies					389:395	most studies	384:395	most studies	384:395	While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries.
24008178	1	15	dep	degrade	111:117	arg1	compromising					185:196	compromising	185:196	compromising marginal integrity	185:215	Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries.
24008178	1	15	dep	degrade	111:117	arg1	leading					221:227	leading	221:227	leading to secondary caries	221:247	Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries.
24008178	5	16	theme	biofilm	742:748	arg1	reactors					750:757	paired CDC biofilm reactors	731:757	paired CDC biofilm reactors	731:757	The disks were incubated for 72 h in paired CDC biofilm reactors, using a previously published protocol.
24008178	14	17	theme	enzymatic	1887:1895	arg1	degradation					1897:1907	chemical and enzymatic degradation	1874:1907	chemical and enzymatic degradation	1874:1907	This was attributed to the inferior dentin coverage by Z100 adhesive, which possibly led to a higher level of chemical and enzymatic degradation.
24008178	9	18	theme	surface	1027:1033	arg1	deformation					1035:1045	The surface deformation	1023:1045	The surface deformation of the disks	1023:1058	The surface deformation of the disks was mapped using digital image correlation to ascertain the fracture origin.
24008178	8	19	theme	interfacial	996:1006	arg1	strength					1013:1020	their interfacial bond strength	990:1020	their interfacial bond strength	990:1020	The disks were fractured under diametral compression to evaluate their interfacial bond strength.
24008178	14	20	theme	chemical	1874:1881	arg1	degradation					1897:1907	chemical and enzymatic degradation	1874:1907	chemical and enzymatic degradation	1874:1907	This was attributed to the inferior dentin coverage by Z100 adhesive, which possibly led to a higher level of chemical and enzymatic degradation.
24008178	12	21	theme	interface	1578:1586	arg1	degradation					1542:1552	preferential degradation	1529:1552	preferential degradation of the composite-dentin interface	1529:1586	Biofilm growth with sucrose pulsing also caused preferential degradation of the composite-dentin interface, depending on the composite/adhesive system used.
24008178	0	22	from	Degradation	0:10	arg1	interface					36:44	the dentin-composite interface	15:44	the dentin-composite interface	15:44	Degradation in the dentin-composite interface subjected to multi-species biofilm challenges.
24008178	16	23	theme	interface	2185:2193	arg1	degradation					2149:2159	the degradation	2145:2159	the degradation of the dentin-composite interface subjected to multi-species biofilm challenge	2145:2238	A clinically relevant in vitro biofilm model was therefore developed, which would effectively allow assessment of the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.
24008178	10	24	theme	interfacial	1272:1282	arg1	degradation					1284:1294	interfacial degradation	1272:1294	interfacial degradation	1272:1294	Fracture surfaces were examined using scanning electron microscopy/energy-dispersive X-ray spectroscopy to assess demineralization and interfacial degradation.
24008178	16	25	theme	in	2053:2054	arg1	model					2070:2074	A clinically relevant in vitro biofilm model	2031:2074	A clinically relevant in vitro biofilm model	2031:2074	A clinically relevant in vitro biofilm model was therefore developed, which would effectively allow assessment of the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.
24008178	15	26	theme	interfacial	2006:2016	arg1	degradation					2018:2028	interfacial degradation	2006:2028	interfacial degradation	2006:2028	The results suggested that factors other than dentin demineralization were also responsible for interfacial degradation.
24008178	9	27	theme	disks	1054:1058	arg1	deformation					1035:1045	The surface deformation	1023:1045	The surface deformation of the disks	1023:1058	The surface deformation of the disks was mapped using digital image correlation to ascertain the fracture origin.
24008178	5	28	theme	paired	731:736	arg1	reactors					750:757	paired CDC biofilm reactors	731:757	paired CDC biofilm reactors	731:757	The disks were incubated for 72 h in paired CDC biofilm reactors, using a previously published protocol.
24008178	3	29	used	used	397:400	arg2	studies					389:395	most studies	384:395	most studies	384:395	While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries.
24008178	1	30	theme	Oral	93:96	arg1	biofilms					98:105	Oral biofilms	93:105	Oral biofilms	93:105	Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries.
24008178	3	31	dep	realistic	457:465	arg1	diverse					468:474	diverse	468:474	diverse	468:474	While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries.
24008178	11	32	theme	mineral	1467:1473	arg1	loss					1475:1478	surface mineral loss	1459:1478	surface mineral loss	1459:1478	Dentin demineralization was greater under sucrose-pulsed biofilms, as the pH dropped <5.5 during pulsing, with LS and Z100 specimens suffering similar degrees of surface mineral loss.
24008178	1	33	from	components	123:132	arg1	restorations					166:177	dental resin-based composite restorations	137:177	dental resin-based composite restorations	137:177	Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries.
24008178	12	34	with	growth	1489:1494	arg1	pulsing					1509:1515	sucrose pulsing	1501:1515	sucrose pulsing	1501:1515	Biofilm growth with sucrose pulsing also caused preferential degradation of the composite-dentin interface, depending on the composite/adhesive system used.
24008178	13	35	theme	frequent	1715:1722	arg1	failure					1733:1739	more frequent cohesive failure	1710:1739	more frequent cohesive failure	1710:1739	Specifically, Z100 specimens showed greater bond strength reduction and more frequent cohesive failure in the adhesive layer.
24008178	9	36	theme	digital	1077:1083	arg1	correlation					1091:1101	digital image correlation	1077:1101	digital image correlation	1077:1101	The surface deformation of the disks was mapped using digital image correlation to ascertain the fracture origin.
24008178	14	37	theme	higher	1858:1863	arg1	level					1865:1869	a higher level	1856:1869	a higher level of chemical and enzymatic degradation	1856:1907	This was attributed to the inferior dentin coverage by Z100 adhesive, which possibly led to a higher level of chemical and enzymatic degradation.
24008178	3	38	theme	caries	577:582	arg1	history					550:556	a history	548:556	a history of early childhood caries	548:582	While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries.
24008178	1	39	theme	composite	156:164	arg1	restorations					166:177	dental resin-based composite restorations	137:177	dental resin-based composite restorations	137:177	Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries.
24008178	3	40	theme	realistic	457:465	arg1	model					484:488	a more realistic, diverse biofilm model	450:488	a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries	450:582	While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries.
24008178	12	41	theme	composite-dentin	1561:1576	arg1	interface					1578:1586	the composite-dentin interface	1557:1586	the composite-dentin interface	1557:1586	Biofilm growth with sucrose pulsing also caused preferential degradation of the composite-dentin interface, depending on the composite/adhesive system used.
24008178	13	42	theme	strength	1687:1694	arg1	reduction					1696:1704	greater bond strength reduction	1674:1704	greater bond strength reduction	1674:1704	Specifically, Z100 specimens showed greater bond strength reduction and more frequent cohesive failure in the adhesive layer.
24008178	0	43	theme	biofilm	73:79	arg1	challenges					81:90	multi-species biofilm challenges	59:90	multi-species biofilm challenges	59:90	Degradation in the dentin-composite interface subjected to multi-species biofilm challenges.
24008178	5	44	theme	published	779:787	arg1	protocol					789:796	a previously published protocol	766:796	a previously published protocol	766:796	The disks were incubated for 72 h in paired CDC biofilm reactors, using a previously published protocol.
24008178	4	45	theme	Dentin-composite	585:600	arg1	disks					602:606	Dentin-composite disks	585:606	Dentin-composite disks	585:606	Dentin-composite disks were made using bovine incisor roots filled with Z100(TM) or Filtek(TM) LS (3M ESPE).
24008178	16	46	theme	degradation	2149:2159	arg1	assessment					2131:2140	assessment	2131:2140	assessment of the degradation of the dentin-composite interface subjected to multi-species biofilm challenge	2131:2238	A clinically relevant in vitro biofilm model was therefore developed, which would effectively allow assessment of the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.
24008178	9	47	theme	fracture	1120:1127	arg1	origin					1129:1134	the fracture origin	1116:1134	the fracture origin	1116:1134	The surface deformation of the disks was mapped using digital image correlation to ascertain the fracture origin.
24008178	10	48	theme	scanning	1175:1182	arg1	spectroscopy					1228:1239	scanning electron microscopy/energy-dispersive X-ray spectroscopy	1175:1239	scanning electron microscopy/energy-dispersive X-ray spectroscopy	1175:1239	Fracture surfaces were examined using scanning electron microscopy/energy-dispersive X-ray spectroscopy to assess demineralization and interfacial degradation.
24008178	12	49	theme	preferential	1529:1540	arg1	degradation					1542:1552	preferential degradation	1529:1552	preferential degradation of the composite-dentin interface	1529:1586	Biofilm growth with sucrose pulsing also caused preferential degradation of the composite-dentin interface, depending on the composite/adhesive system used.
24008178	11	50	theme	Z100	1415:1418	arg1	specimens					1420:1428	LS and Z100 specimens	1408:1428	LS and Z100 specimens suffering similar degrees of surface mineral loss	1408:1478	Dentin demineralization was greater under sucrose-pulsed biofilms, as the pH dropped <5.5 during pulsing, with LS and Z100 specimens suffering similar degrees of surface mineral loss.
24008178	3	51	theme	early	561:565	arg1	caries					577:582	early childhood caries	561:582	early childhood caries	561:582	While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries.
24008178	7	52	theme	saliva-only	869:879	arg1	run					899:901	run	899:901	run	899:901	A sterile saliva-only control group was run with sucrose pulsing.
24008178	7	52	theme	saliva-only	869:879	arg1	group					889:893	A sterile saliva-only control group	859:893	A sterile saliva-only control group	859:893	A sterile saliva-only control group was run with sucrose pulsing.
24008178	11	53	theme	LS	1408:1409	arg1	specimens					1420:1428	LS and Z100 specimens	1408:1428	LS and Z100 specimens suffering similar degrees of surface mineral loss	1408:1478	Dentin demineralization was greater under sucrose-pulsed biofilms, as the pH dropped <5.5 during pulsing, with LS and Z100 specimens suffering similar degrees of surface mineral loss.
24008178	12	54	theme	sucrose	1501:1507	arg1	pulsing					1509:1515	sucrose pulsing	1501:1515	sucrose pulsing	1501:1515	Biofilm growth with sucrose pulsing also caused preferential degradation of the composite-dentin interface, depending on the composite/adhesive system used.
24008178	4	55	theme	incisor	631:637	arg1	roots					639:643	bovine incisor roots	624:643	bovine incisor roots filled with Z100(TM) or Filtek(TM) LS (3M ESPE)	624:691	Dentin-composite disks were made using bovine incisor roots filled with Z100(TM) or Filtek(TM) LS (3M ESPE).
24008178	10	56	theme	microscopy/energy-dispersive	1193:1220	arg1	spectroscopy					1228:1239	scanning electron microscopy/energy-dispersive X-ray spectroscopy	1175:1239	scanning electron microscopy/energy-dispersive X-ray spectroscopy	1175:1239	Fracture surfaces were examined using scanning electron microscopy/energy-dispersive X-ray spectroscopy to assess demineralization and interfacial degradation.
24008178	8	57	theme	diametral	956:964	arg1	compression					966:976	diametral compression	956:976	diametral compression to evaluate their interfacial bond strength	956:1020	The disks were fractured under diametral compression to evaluate their interfacial bond strength.
24008178	2	58	theme	dentin-composite	306:321	arg1	interface					323:331	the dentin-composite interface	302:331	the dentin-composite interface challenged with multi-species oral biofilms	302:375	This study investigates the mechanical integrity of the dentin-composite interface challenged with multi-species oral biofilms.
24008178	14	59	theme	inferior	1791:1798	arg1	coverage					1807:1814	the inferior dentin coverage	1787:1814	the inferior dentin coverage	1787:1814	This was attributed to the inferior dentin coverage by Z100 adhesive, which possibly led to a higher level of chemical and enzymatic degradation.
24008178	15	60	theme	dentin	1956:1961	arg1	demineralization					1963:1978	dentin demineralization	1956:1978	dentin demineralization	1956:1978	The results suggested that factors other than dentin demineralization were also responsible for interfacial degradation.
24008178	2	61	theme	multi-species	349:361	arg1	biofilms					368:375	multi-species oral biofilms	349:375	multi-species oral biofilms	349:375	This study investigates the mechanical integrity of the dentin-composite interface challenged with multi-species oral biofilms.
24008178	16	62	dep	in	2053:2054	arg1	vitro					2056:2060	vitro	2056:2060	vitro	2056:2060	A clinically relevant in vitro biofilm model was therefore developed, which would effectively allow assessment of the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.
24008178	15	63	theme	other	1945:1949	arg1	factors					1937:1943	factors	1937:1943	factors other than dentin demineralization	1937:1978	The results suggested that factors other than dentin demineralization were also responsible for interfacial degradation.
24008178	1	64	theme	secondary	232:240	arg1	caries					242:247	secondary caries	232:247	secondary caries	232:247	Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries.
24008178	5	65	from	h	726:726	arg1	reactors					750:757	paired CDC biofilm reactors	731:757	paired CDC biofilm reactors	731:757	The disks were incubated for 72 h in paired CDC biofilm reactors, using a previously published protocol.
24008178	1	66	theme	dental	137:142	arg1	restorations					166:177	dental resin-based composite restorations	137:177	dental resin-based composite restorations	137:177	Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries.
24008178	12	67	theme	composite/adhesive	1606:1623	arg1	system					1625:1630	the composite/adhesive system	1602:1630	the composite/adhesive system used	1602:1635	Biofilm growth with sucrose pulsing also caused preferential degradation of the composite-dentin interface, depending on the composite/adhesive system used.
24008178	5	68	theme	CDC	738:740	arg1	reactors					750:757	paired CDC biofilm reactors	731:757	paired CDC biofilm reactors	731:757	The disks were incubated for 72 h in paired CDC biofilm reactors, using a previously published protocol.
24008178	16	69	theme	biofilm	2222:2228	arg1	challenge					2230:2238	multi-species biofilm challenge	2208:2238	multi-species biofilm challenge	2208:2238	A clinically relevant in vitro biofilm model was therefore developed, which would effectively allow assessment of the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.
24008178	2	70	theme	mechanical	278:287	arg1	integrity					289:297	the mechanical integrity	274:297	the mechanical integrity of the dentin-composite interface challenged with multi-species oral biofilms	274:375	This study investigates the mechanical integrity of the dentin-composite interface challenged with multi-species oral biofilms.
24008178	14	71	theme	degradation	1897:1907	arg1	level					1865:1869	a higher level	1856:1869	a higher level of chemical and enzymatic degradation	1856:1907	This was attributed to the inferior dentin coverage by Z100 adhesive, which possibly led to a higher level of chemical and enzymatic degradation.
24008178	8	72	theme	bond	1008:1011	arg1	strength					1013:1020	their interfacial bond strength	990:1020	their interfacial bond strength	990:1020	The disks were fractured under diametral compression to evaluate their interfacial bond strength.
24008178	16	73	theme	dentin-composite	2168:2183	arg1	interface					2185:2193	the dentin-composite interface	2164:2193	the dentin-composite interface subjected to multi-species biofilm challenge	2164:2238	A clinically relevant in vitro biofilm model was therefore developed, which would effectively allow assessment of the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.
24008178	11	74	theme	loss	1475:1478	arg1	degrees					1448:1454	similar degrees	1440:1454	similar degrees of surface mineral loss	1440:1478	Dentin demineralization was greater under sucrose-pulsed biofilms, as the pH dropped <5.5 during pulsing, with LS and Z100 specimens suffering similar degrees of surface mineral loss.
24008178	3	75	theme	single-species	402:415	arg1	biofilms					417:424	single-species biofilms	402:424	single-species biofilms	402:424	While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries.
24008178	13	76	theme	adhesive	1748:1755	arg1	layer					1757:1761	the adhesive layer	1744:1761	the adhesive layer	1744:1761	Specifically, Z100 specimens showed greater bond strength reduction and more frequent cohesive failure in the adhesive layer.
24008178	11	77	theme	surface	1459:1465	arg1	loss					1475:1478	surface mineral loss	1459:1478	surface mineral loss	1459:1478	Dentin demineralization was greater under sucrose-pulsed biofilms, as the pH dropped <5.5 during pulsing, with LS and Z100 specimens suffering similar degrees of surface mineral loss.
24008178	7	78	theme	sucrose	908:914	arg1	pulsing					916:922	sucrose pulsing	908:922	sucrose pulsing	908:922	A sterile saliva-only control group was run with sucrose pulsing.
24008178	16	79	theme	multi-species	2208:2220	arg1	challenge					2230:2238	multi-species biofilm challenge	2208:2238	multi-species biofilm challenge	2208:2238	A clinically relevant in vitro biofilm model was therefore developed, which would effectively allow assessment of the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.
24008178	3	80	theme	biofilm	476:482	arg1	model					484:488	a more realistic, diverse biofilm model	450:488	a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries	450:582	While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries.
24008178	0	81	theme	dentin-composite	19:34	arg1	interface					36:44	the dentin-composite interface	15:44	the dentin-composite interface	15:44	Degradation in the dentin-composite interface subjected to multi-species biofilm challenges.
24008178	3	82	theme	present	431:437	arg1	study					439:443	the present study	427:443	the present study	427:443	While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries.
24008178	4	83	theme	bovine	624:629	arg1	roots					639:643	bovine incisor roots	624:643	bovine incisor roots filled with Z100(TM) or Filtek(TM) LS (3M ESPE)	624:691	Dentin-composite disks were made using bovine incisor roots filled with Z100(TM) or Filtek(TM) LS (3M ESPE).
24008178	13	84	theme	cohesive	1724:1731	arg1	failure					1733:1739	more frequent cohesive failure	1710:1739	more frequent cohesive failure	1710:1739	Specifically, Z100 specimens showed greater bond strength reduction and more frequent cohesive failure in the adhesive layer.
24008178	9	85	theme	image	1085:1089	arg1	correlation					1091:1101	digital image correlation	1077:1101	digital image correlation	1077:1101	The surface deformation of the disks was mapped using digital image correlation to ascertain the fracture origin.
24008178	3	86	used	used	445:448	arg2	study					439:443	the present study	427:443	the present study	427:443	While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries.
24008178	13	87	theme	greater	1674:1680	arg1	reduction					1696:1704	greater bond strength reduction	1674:1704	greater bond strength reduction	1674:1704	Specifically, Z100 specimens showed greater bond strength reduction and more frequent cohesive failure in the adhesive layer.
24008178	0	88	theme	multi-species	59:71	arg1	challenges					81:90	multi-species biofilm challenges	59:90	multi-species biofilm challenges	59:90	Degradation in the dentin-composite interface subjected to multi-species biofilm challenges.
24008178	10	89	theme	Fracture	1137:1144	arg1	surfaces					1146:1153	Fracture surfaces	1137:1153	Fracture surfaces	1137:1153	Fracture surfaces were examined using scanning electron microscopy/energy-dispersive X-ray spectroscopy to assess demineralization and interfacial degradation.
24008178	11	90	theme	Dentin	1297:1302	arg1	demineralization					1304:1319	Dentin demineralization	1297:1319	Dentin demineralization	1297:1319	Dentin demineralization was greater under sucrose-pulsed biofilms, as the pH dropped <5.5 during pulsing, with LS and Z100 specimens suffering similar degrees of surface mineral loss.
24008178	7	91	theme	control	881:887	arg1	run					899:901	run	899:901	run	899:901	A sterile saliva-only control group was run with sucrose pulsing.
24008178	7	91	theme	control	881:887	arg1	group					889:893	A sterile saliva-only control group	859:893	A sterile saliva-only control group	859:893	A sterile saliva-only control group was run with sucrose pulsing.
24008178	16	92	theme	biofilm	2062:2068	arg1	model					2070:2074	A clinically relevant in vitro biofilm model	2031:2074	A clinically relevant in vitro biofilm model	2031:2074	A clinically relevant in vitro biofilm model was therefore developed, which would effectively allow assessment of the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.
24008178	7	93	theme	sterile	861:867	arg1	run					899:901	run	899:901	run	899:901	A sterile saliva-only control group was run with sucrose pulsing.
24008178	7	93	theme	sterile	861:867	arg1	group					889:893	A sterile saliva-only control group	859:893	A sterile saliva-only control group	859:893	A sterile saliva-only control group was run with sucrose pulsing.
24008178	14	94	theme	adhesive	1824:1831	arg1	Z100					1819:1822	Z100 adhesive	1819:1831	Z100 adhesive	1819:1831	This was attributed to the inferior dentin coverage by Z100 adhesive, which possibly led to a higher level of chemical and enzymatic degradation.
24008178	16	95	theme	relevant	2044:2051	arg1	model					2070:2074	A clinically relevant in vitro biofilm model	2031:2074	A clinically relevant in vitro biofilm model	2031:2074	A clinically relevant in vitro biofilm model was therefore developed, which would effectively allow assessment of the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.
24008178	3	96	theme	childhood	567:575	arg1	caries					577:582	early childhood caries	561:582	early childhood caries	561:582	While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries.
26930975	7	0	theme	respective	1380:1389	arg1	advantages					1391:1400	respective advantages	1380:1400	respective advantages	1380:1400	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	6	1	theme	relative	1157:1164	arg1	contents					1166:1173	the relative contents	1153:1173	the relative contents of sibiricaxanthone A,3,6'-disinapoly sucrose and senegin III	1153:1235	In addition, the relative contents of sibiricaxanthone A,3,6'-disinapoly sucrose and senegin III showed significant differences among FY, JY1 and FY2.
26930975	5	2	theme	Polygala	1087:1094	arg1	varieties					1107:1115	Polygala tenuifolia varieties	1087:1115	Polygala tenuifolia varieties in the PCA score plot	1087:1137	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	3	3	theme	obtained	504:511	arg1	data					513:516	The obtained data	500:516	The obtained data	500:516	The obtained data were analyzed using Principal Component Analysis (PCA) and other statistical analysis methods, and differential metabolites were further figured out.
26930975	4	4	from	varieties	872:880	arg1	plot					899:902	the PCA score plot	885:902	the PCA score plot	885:902	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	4	5	theme	tenuifoliose	783:794	arg1	K					796:796	tenuifoliose K	783:796	tenuifoliose K	783:796	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	5	6	from	plot	1134:1137	arg1	separation					1073:1082	the separation	1069:1082	the separation of Polygala tenuifolia varieties in the PCA score plot	1069:1137	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	5	7	from	separation	1073:1082	arg1	plot					1134:1137	the PCA score plot	1120:1137	the PCA score plot	1120:1137	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	1	8	theme	FenYuan	196:202	arg1	differences					134:144	The chemical differences	121:144	The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY)	121:239	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	5	9	from	varieties	1107:1115	arg1	plot					1134:1137	the PCA score plot	1120:1137	the PCA score plot	1120:1137	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	5	10	theme	tenuifoliside	970:982	arg1	A					984:984	tenuifoliside A	970:984	tenuifoliside A	970:984	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	4	11	theme	tenuifolia	861:870	arg1	varieties					872:880	Polygala tenuifolia varieties	852:880	Polygala tenuifolia varieties in the PCA score plot	852:902	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	7	12	theme	Polygala	1512:1519	arg1	tenuifolia					1521:1530	Polygala tenuifolia	1512:1530	Polygala tenuifolia	1512:1530	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	6	13	theme	A,3,6'-disinapoly	1195:1211	arg1	sucrose					1213:1219	sibiricaxanthone A,3,6'-disinapoly sucrose	1178:1219	sibiricaxanthone A,3,6'-disinapoly sucrose	1178:1219	In addition, the relative contents of sibiricaxanthone A,3,6'-disinapoly sucrose and senegin III showed significant differences among FY, JY1 and FY2.
26930975	3	14	theme	Component	548:556	arg1	Analysis					558:565	Principal Component Analysis	538:565	Principal Component Analysis (PCA)	538:571	The obtained data were analyzed using Principal Component Analysis (PCA) and other statistical analysis methods, and differential metabolites were further figured out.
26930975	3	14	theme	Component	548:556	arg1	PCA					568:570	PCA	568:570	PCA	568:570	The obtained data were analyzed using Principal Component Analysis (PCA) and other statistical analysis methods, and differential metabolites were further figured out.
26930975	4	15	from	oligosaccharides	757:772	arg1	JY1					802:804	JY1	802:804	JY1	802:804	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	4	15	from	oligosaccharides	757:772	arg1	FY2					810:812	FY2	810:812	FY2	810:812	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	4	16	dep	esters	701:706	arg1	such					709:712	such	709:712	such	709:712	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	3	17	theme	other	577:581	arg1	methods					604:610	other statistical analysis methods	577:610	other statistical analysis methods	577:610	The obtained data were analyzed using Principal Component Analysis (PCA) and other statistical analysis methods, and differential metabolites were further figured out.
26930975	2	18	theme	mass	461:464	arg1	spectrometry					466:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry	383:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	383:497	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	2	18	theme	mass	461:464	arg1	MS					486:487	Q-TOF MS	480:487	Q-TOF MS	480:487	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	2	19	theme	spectrometry	466:477	arg1	analysis					490:497	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	383:497	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	383:497	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	1	20	dep	OBJECTIVE	111:119	arg1	studied					246:252	studied	246:252	studied	246:252	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	0	21	theme	Different	69:77	arg1	Varieties					99:107	Different Polygala tenuifolia Varieties	69:107	Different Polygala tenuifolia Varieties	69:107	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties].
26930975	5	22	theme	tenuifoliose	1017:1028	arg1	A					1030:1030	tenuifoliose A	1017:1030	tenuifoliose A	1017:1030	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	4	23	dep	sibiricoses	717:727	arg1	B					750:750	tenuifoliside B	736:750	tenuifoliside B	736:750	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	4	23	dep	sibiricoses	717:727	arg1	sibiricoses					717:727	sibiricoses A5 and tenuifoliside B	717:750	sibiricoses A5 and tenuifoliside B	717:750	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	4	23	dep	sibiricoses	717:727	arg1	A5					729:730	A5	729:730	A5	729:730	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	2	24	theme	time-of-flight	446:459	arg1	spectrometry					466:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry	383:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	383:497	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	2	24	theme	time-of-flight	446:459	arg1	MS					486:487	Q-TOF MS	480:487	Q-TOF MS	480:487	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	0	25	theme	tenuifolia	88:97	arg1	Varieties					99:107	Different Polygala tenuifolia Varieties	69:107	Different Polygala tenuifolia Varieties	69:107	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties].
26930975	1	26	theme	Polygala	305:312	arg1	tenuifolia					314:323	Polygala tenuifolia	305:323	Polygala tenuifolia	305:323	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	2	27	dep	METHODS	326:332	arg1	subjected					370:378	subjected	370:378	were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	365:497	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	1	28	theme	Polygala	149:156	arg1	varieties-JinYuan					169:185	Polygala tenuifolia varieties-JinYuan 1	149:187	Polygala tenuifolia varieties-JinYuan 1 (JY1)	149:193	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	1	28	theme	Polygala	149:156	arg1	JY1					190:192	JY1	190:192	JY1	190:192	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	2	29	theme	Q-TOF	480:484	arg1	spectrometry					466:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry	383:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	383:497	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	2	29	theme	Q-TOF	480:484	arg1	MS					486:487	Q-TOF MS	480:487	Q-TOF MS	480:487	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	0	30	from	uiterences	55:64	arg1	Varieties					99:107	Different Polygala tenuifolia Varieties	69:107	Different Polygala tenuifolia Varieties	69:107	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties].
26930975	2	31	theme	FY2	354:356	arg1	samples					338:344	The samples	334:344	The samples of JY1, FY2 and FY	334:363	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	5	32	theme	varieties	1107:1115	arg1	separation					1073:1082	the separation	1069:1082	the separation of Polygala tenuifolia varieties in the PCA score plot	1069:1137	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	7	33	theme	compounds	1569:1577	arg1	contents					1545:1552	the contents	1541:1552	the contents of some active compounds	1541:1577	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	2	34	theme	liquid	406:411	arg1	spectrometry					466:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry	383:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	383:497	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	2	34	theme	liquid	406:411	arg1	MS					486:487	Q-TOF MS	480:487	Q-TOF MS	480:487	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	4	35	theme	sucrose	693:699	arg1	esters					701:706	sucrose esters	693:706	sucrose esters (such as sibiricoses A5 and tenuifoliside B)	693:751	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	4	35	theme	sucrose	693:699	arg1	FY					690:691	FY	690:691	FY	690:691	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	2	36	theme	JY1	349:351	arg1	samples					338:344	The samples	334:344	The samples of JY1, FY2 and FY	334:363	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	0	37	theme	[UPLC/Q-TOF	0:10	arg1	Analysis					34:41	[UPLC/Q-TOF MS-Based Metabolomics Analysis	0:41	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties	0:107	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties].
26930975	2	38	theme	ultra-high	383:392	arg1	spectrometry					466:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry	383:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	383:497	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	2	38	theme	ultra-high	383:392	arg1	MS					486:487	Q-TOF MS	480:487	Q-TOF MS	480:487	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	0	39	theme	Metabolomics	21:32	arg1	Analysis					34:41	[UPLC/Q-TOF MS-Based Metabolomics Analysis	0:41	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties	0:107	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties].
26930975	7	40	theme	Polygala	1309:1316	arg1	varieties					1329:1337	new Polygala tenuifolia varieties	1305:1337	new Polygala tenuifolia varieties	1305:1337	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	2	41	theme	FY	362:363	arg1	samples					338:344	The samples	334:344	The samples of JY1, FY2 and FY	334:363	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	3	42	theme	analysis	595:602	arg1	methods					604:610	other statistical analysis methods	577:610	other statistical analysis methods	577:610	The obtained data were analyzed using Principal Component Analysis (PCA) and other statistical analysis methods, and differential metabolites were further figured out.
26930975	0	43	theme	uiterences	55:64	arg1	Analysis					34:41	[UPLC/Q-TOF MS-Based Metabolomics Analysis	0:41	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties	0:107	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties].
26930975	4	44	theme	tenuifoliside	736:748	arg1	B					750:750	tenuifoliside B	736:750	tenuifoliside B	736:750	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	4	44	theme	tenuifoliside	736:748	arg1	sibiricoses					717:727	sibiricoses A5 and tenuifoliside B	717:750	sibiricoses A5 and tenuifoliside B	717:750	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	1	45	theme	varieties-JinYuan	169:185	arg1	differences					134:144	The chemical differences	121:144	The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY)	121:239	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	3	46	theme	differential	617:628	arg1	metabolites					630:640	differential metabolites	617:640	differential metabolites	617:640	The obtained data were analyzed using Principal Component Analysis (PCA) and other statistical analysis methods, and differential metabolites were further figured out.
26930975	5	47	theme	PCA	1124:1126	arg1	plot					1134:1137	the PCA score plot	1120:1137	the PCA score plot	1120:1137	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	7	48	theme	certain	1356:1362	arg1	differences					1364:1374	certain differences	1356:1374	certain differences	1356:1374	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	5	49	theme	tenuifolia	1096:1105	arg1	varieties					1107:1115	Polygala tenuifolia varieties	1087:1115	Polygala tenuifolia varieties in the PCA score plot	1087:1137	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	7	50	theme	tenuifolia	1318:1327	arg1	varieties					1329:1337	new Polygala tenuifolia varieties	1305:1337	new Polygala tenuifolia varieties	1305:1337	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	7	51	theme	new	1305:1307	arg1	varieties					1329:1337	new Polygala tenuifolia varieties	1305:1337	new Polygala tenuifolia varieties	1305:1337	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	5	52	dep	esters	934:939	arg1	such					942:945	such	942:945	such	942:945	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	7	53	dep	CONCLUSION	1291:1300	arg1	varieties					1329:1337	new Polygala tenuifolia varieties	1305:1337	new Polygala tenuifolia varieties	1305:1337	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	7	54	theme	tenuifolia	1521:1530	arg1	breeding					1500:1507	the directional breeding	1484:1507	the directional breeding of Polygala tenuifolia based on the contents of some active compounds	1484:1577	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	0	55	from	Varieties	99:107	arg1	Analysis					34:41	[UPLC/Q-TOF MS-Based Metabolomics Analysis	0:41	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties	0:107	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties].
26930975	6	56	theme	senegin	1225:1231	arg1	contents					1166:1173	the relative contents	1153:1173	the relative contents of sibiricaxanthone A,3,6'-disinapoly sucrose and senegin III	1153:1235	In addition, the relative contents of sibiricaxanthone A,3,6'-disinapoly sucrose and senegin III showed significant differences among FY, JY1 and FY2.
26930975	5	57	from	esters	934:939	arg1	FY2					1040:1042	the FY2	1036:1042	the FY2	1036:1042	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	4	58	dep	oligosaccharides	757:772	arg1	such					775:778	such	775:778	such	775:778	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	4	59	theme	Polygala	852:859	arg1	varieties					872:880	Polygala tenuifolia varieties	852:880	Polygala tenuifolia varieties in the PCA score plot	852:902	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	6	60	theme	sucrose	1213:1219	arg1	contents					1166:1173	the relative contents	1153:1173	the relative contents of sibiricaxanthone A,3,6'-disinapoly sucrose and senegin III	1153:1235	In addition, the relative contents of sibiricaxanthone A,3,6'-disinapoly sucrose and senegin III showed significant differences among FY, JY1 and FY2.
26930975	4	61	from	separation	838:847	arg1	plot					899:902	the PCA score plot	885:902	the PCA score plot	885:902	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	3	62	theme	Principal	538:546	arg1	Analysis					558:565	Principal Component Analysis	538:565	Principal Component Analysis (PCA)	538:571	The obtained data were analyzed using Principal Component Analysis (PCA) and other statistical analysis methods, and differential metabolites were further figured out.
26930975	3	62	theme	Principal	538:546	arg1	PCA					568:570	PCA	568:570	PCA	568:570	The obtained data were analyzed using Principal Component Analysis (PCA) and other statistical analysis methods, and differential metabolites were further figured out.
26930975	1	63	theme	chemical	125:132	arg1	differences					134:144	The chemical differences	121:144	The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY)	121:239	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	7	64	theme	directional	1488:1498	arg1	breeding					1500:1507	the directional breeding	1484:1507	the directional breeding of Polygala tenuifolia based on the contents of some active compounds	1484:1577	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	6	65	theme	sibiricaxanthone	1178:1193	arg1	sucrose					1213:1219	sibiricaxanthone A,3,6'-disinapoly sucrose	1178:1219	sibiricaxanthone A,3,6'-disinapoly sucrose	1178:1219	In addition, the relative contents of sibiricaxanthone A,3,6'-disinapoly sucrose and senegin III showed significant differences among FY, JY1 and FY2.
26930975	5	66	theme	tenuifoliside	950:962	arg1	B					964:964	tenuifoliside B	950:964	tenuifoliside B	950:964	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	7	67	contain	had	1352:1354	arg1	FY2					1348:1350	FY2	1348:1350	FY2	1348:1350	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	7	67	contain	had	1352:1354	arg2	advantages					1391:1400	respective advantages	1380:1400	respective advantages	1380:1400	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	7	67	contain	had	1352:1354	arg1	JY1					1340:1342	JY1	1340:1342	JY1	1340:1342	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	7	67	contain	had	1352:1354	arg2	differences					1364:1374	certain differences	1356:1374	certain differences	1356:1374	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	0	68	theme	Polygala	79:86	arg1	Varieties					99:107	Different Polygala tenuifolia Varieties	69:107	Different Polygala tenuifolia Varieties	69:107	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties].
26930975	1	69	theme	tenuifolia	158:167	arg1	varieties-JinYuan					169:185	Polygala tenuifolia varieties-JinYuan 1	149:187	Polygala tenuifolia varieties-JinYuan 1 (JY1)	149:193	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	1	69	theme	tenuifolia	158:167	arg1	JY1					190:192	JY1	190:192	JY1	190:192	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	4	70	theme	score	893:897	arg1	plot					899:902	the PCA score plot	885:902	the PCA score plot	885:902	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	0	71	from	Analysis	34:41	arg1	Varieties					99:107	Different Polygala tenuifolia Varieties	69:107	Different Polygala tenuifolia Varieties	69:107	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties].
26930975	2	72	theme	quadrupole	435:444	arg1	spectrometry					466:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry	383:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	383:497	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	2	72	theme	quadrupole	435:444	arg1	MS					486:487	Q-TOF MS	480:487	Q-TOF MS	480:487	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	1	73	theme	tenuifolia	314:323	arg1	breeding					293:300	the breeding	289:300	the breeding of Polygala tenuifolia	289:323	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	4	74	theme	varieties	872:880	arg1	separation					838:847	the separation	834:847	the separation of Polygala tenuifolia varieties in the PCA score plot	834:902	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	7	75	dep	breeding	1500:1507	arg1	support					1472:1478	support	1472:1478	support	1472:1478	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	4	76	theme	PCA	889:891	arg1	plot					899:902	the PCA score plot	885:902	the PCA score plot	885:902	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	0	77	theme	MS-Based	12:19	arg1	Analysis					34:41	[UPLC/Q-TOF MS-Based Metabolomics Analysis	0:41	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties	0:107	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties].
26930975	1	78	theme	FenYang	228:234	arg1	differences					134:144	The chemical differences	121:144	The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY)	121:239	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	2	79	theme	performance	394:404	arg1	spectrometry					466:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry	383:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	383:497	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	2	79	theme	performance	394:404	arg1	MS					486:487	Q-TOF MS	480:487	Q-TOF MS	480:487	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	5	80	from	oligosaccharides	991:1006	arg1	FY2					1040:1042	the FY2	1036:1042	the FY2	1036:1042	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	7	81	theme	active	1562:1567	arg1	compounds					1569:1577	some active compounds	1557:1577	some active compounds	1557:1577	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	0	82	theme	Chemical	46:53	arg1	uiterences					55:64	Chemical uiterences	46:64	Chemical uiterences in Different Polygala tenuifolia Varieties	46:107	[UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical uiterences in Different Polygala tenuifolia Varieties].
26930975	5	83	theme	sugar	928:932	arg1	esters					934:939	The sugar esters	924:939	The sugar esters (such as tenuifoliside B and tenuifoliside A)	924:985	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	2	84	theme	UPLC	429:432	arg1	spectrometry					466:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry	383:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	383:497	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	2	84	theme	UPLC	429:432	arg1	MS					486:487	Q-TOF MS	480:487	Q-TOF MS	480:487	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	3	85	theme	statistical	583:593	arg1	methods					604:610	other statistical analysis methods	577:610	other statistical analysis methods	577:610	The obtained data were analyzed using Principal Component Analysis (PCA) and other statistical analysis methods, and differential metabolites were further figured out.
26930975	4	86	from	plot	899:902	arg1	separation					838:847	the separation	834:847	the separation of Polygala tenuifolia varieties in the PCA score plot	834:902	RESULTS Compared with FY,sucrose esters (such as sibiricoses A5 and tenuifoliside B) and oligosaccharides (such as tenuifoliose K) in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	6	87	theme	significant	1244:1254	arg1	differences					1256:1266	significant differences	1244:1266	significant differences among FY, JY1 and FY2	1244:1288	In addition, the relative contents of sibiricaxanthone A,3,6'-disinapoly sucrose and senegin III showed significant differences among FY, JY1 and FY2.
26930975	2	88	theme	chromatography	413:426	arg1	spectrometry					466:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry	383:477	ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis	383:497	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	2	88	theme	chromatography	413:426	arg1	MS					486:487	Q-TOF MS	480:487	Q-TOF MS	480:487	METHODS The samples of JY1, FY2 and FY were subjected to ultra-high performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis.
26930975	5	89	dep	oligosaccharides	991:1006	arg1	such					1009:1012	such	1009:1012	such	1009:1012	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26930975	7	90	theme	chemical	1409:1416	arg1	composition					1418:1428	the chemical composition	1405:1428	the chemical composition	1405:1428	CONCLUSION As new Polygala tenuifolia varieties, JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.
26930975	1	91	theme	traditional	216:226	arg1	FenYang					228:234	traditional FenYang	216:234	traditional FenYang (FY)	216:239	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	1	91	theme	traditional	216:226	arg1	FY					237:238	FY	237:238	FY	237:238	OBJECTIVE The chemical differences of Polygala tenuifolia varieties-JinYuan 1 (JY1), FenYuan 2 (FY2) and traditional FenYang (FY) were studied, in order to provide reference for the breeding of Polygala tenuifolia.
26930975	5	92	theme	score	1128:1132	arg1	plot					1134:1137	the PCA score plot	1120:1137	the PCA score plot	1120:1137	Compared with JYl, The sugar esters (such as tenuifoliside B and tenuifoliside A) and oligosaccharides( such as tenuifoliose A) in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot.
26374493	0	0	theme	roasted	77:83	arg1	chickpeas					85:93	roasted chickpeas	77:93	roasted chickpeas	77:93	Chemical composition, nutritional value and in vitro starch digestibility of roasted chickpeas.
26374493	6	1	theme	P	885:885	arg1	<					887:887	P < 0.05	885:892	P < 0.05	885:892	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	9	2	contain	have	1289:1292	arg2	quality					1311:1317	good nutritional quality	1294:1317	good nutritional quality	1294:1317	CONCLUSION The results indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response.
26374493	9	2	contain	have	1289:1292	arg1	chickpea					1260:1267	heat-treated chickpea	1247:1267	heat-treated chickpea	1247:1267	CONCLUSION The results indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response.
26374493	9	2	contain	have	1289:1292	arg1	leblebi					1281:1287	roasted leblebi	1273:1287	roasted leblebi	1273:1287	CONCLUSION The results indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response.
26374493	9	2	contain	have	1289:1292	arg2	response					1336:1343	low glycemic response	1323:1343	low glycemic response	1323:1343	CONCLUSION The results indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response.
26374493	8	3	theme	resistant	1055:1063	arg1	starch					1065:1070	High resistant starch	1050:1070	High resistant starch content (28.28% to 30.20%)	1050:1097	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	4	4	theme	nutritional	542:552	arg1	value					554:558	the nutritional value	538:558	the nutritional value of snack foods	538:573	However, consumers are increasingly concerned with the nutritional value of snack foods.
26374493	8	5	theme	%	1086:1086	arg1	%					1096:1096	28.28% to 30.20%	1081:1096	28.28% to 30.20%	1081:1096	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	8	5	theme	%	1086:1086	arg1	content					1072:1078	High resistant starch content	1050:1078	High resistant starch content (28.28% to 30.20%)	1050:1097	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	10	6	theme	sodium	1380:1385	arg1	content					1387:1393	relatively high sodium content	1364:1393	relatively high sodium content	1364:1393	White leblebi had relatively high sodium content and glycemic response.
26374493	6	7	theme	fiber	793:797	arg1	content					799:805	soluble dietary fiber content	777:805	soluble dietary fiber content	777:805	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	1	8	theme	due	162:164	arg1	food					157:160	a wholesome and nutritious food	130:160	a wholesome and nutritious food due to its nutritional properties and glycemic response	130:216	BACKGROUND Chickpea is considered a wholesome and nutritious food due to its nutritional properties and glycemic response.
26374493	11	9	theme	Chemical	1436:1443	arg1	Industry					1445:1452	Chemical Industry	1436:1452	Chemical Industry	1436:1452	© 2015 Society of Chemical Industry.
26374493	5	10	theme	leblebi	706:712	arg1	properties					625:634	digestibility properties	611:634	digestibility properties	611:634	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	5	10	theme	leblebi	706:712	arg1	RESULTS					576:582	RESULTS Nutritional components and digestibility properties	576:634	RESULTS	576:582	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	6	11	dep	higher	895:900	arg1	<					887:887	P < 0.05	885:892	P < 0.05	885:892	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	5	12	theme	single	639:644	arg1	chickpea					670:677	single and double heat-treated chickpea	639:677	single and double heat-treated chickpea	639:677	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	6	13	theme	dietary	785:791	arg1	content					799:805	soluble dietary fiber content	777:805	soluble dietary fiber content	777:805	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	0	14	theme	chickpeas	85:93	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, nutritional value and in vitro starch digestibility of roasted chickpeas.
26374493	0	14	theme	chickpeas	85:93	arg1	value					34:38	nutritional value	22:38	nutritional value	22:38	Chemical composition, nutritional value and in vitro starch digestibility of roasted chickpeas.
26374493	0	14	theme	chickpeas	85:93	arg1	digestibility					60:72	in vitro starch digestibility	44:72	in vitro starch digestibility	44:72	Chemical composition, nutritional value and in vitro starch digestibility of roasted chickpeas.
26374493	9	15	theme	good	1294:1297	arg1	quality					1311:1317	good nutritional quality	1294:1317	good nutritional quality	1294:1317	CONCLUSION The results indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response.
26374493	6	16	theme	soluble	777:783	arg1	content					799:805	soluble dietary fiber content	777:805	soluble dietary fiber content	777:805	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	8	17	dep	%	1096:1096	arg1	to					1088:1089	to	1088:1089	to	1088:1089	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	7	18	dep	significantly	965:977	arg1	<					982:982	P < 0.05	980:987	P < 0.05	980:987	Heat treatment and processing significantly (P < 0.05) altered the viscosity and starch properties of the samples.
26374493	10	19	contain	had	1360:1362	arg2	content					1387:1393	relatively high sodium content	1364:1393	relatively high sodium content	1364:1393	White leblebi had relatively high sodium content and glycemic response.
26374493	10	19	contain	had	1360:1362	arg2	response					1408:1415	glycemic response	1399:1415	glycemic response	1399:1415	White leblebi had relatively high sodium content and glycemic response.
26374493	10	19	contain	had	1360:1362	arg1	leblebi					1352:1358	White leblebi	1346:1358	White leblebi	1346:1358	White leblebi had relatively high sodium content and glycemic response.
26374493	5	20	theme	white	718:722	arg1	leblebi					724:730	white leblebi	718:730	white leblebi	718:730	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	3	21	from	point	358:362	arg1	desirable					379:387	desirable	379:387	desirable	379:387	From the consumers' point of view, it is desirable to improve texture and palatability of the chickpea by the processing steps used to make leblebi.
26374493	5	22	theme	Nutritional	584:594	arg1	components					596:605	Nutritional components	584:605	Nutritional components	584:605	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	1	23	theme	glycemic	200:207	arg1	response					209:216	glycemic response	200:216	glycemic response	200:216	BACKGROUND Chickpea is considered a wholesome and nutritious food due to its nutritional properties and glycemic response.
26374493	5	24	theme	leblebi	724:730	arg1	properties					625:634	digestibility properties	611:634	digestibility properties	611:634	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	5	24	theme	leblebi	724:730	arg1	RESULTS					576:582	RESULTS Nutritional components and digestibility properties	576:634	RESULTS	576:582	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	6	25	theme	other	849:853	arg1	samples					855:861	the other samples	845:861	the other samples	845:861	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	8	26	from	content	1072:1078	arg1	chickpeas					1165:1173	heat-treated chickpeas	1152:1173	heat-treated chickpeas	1152:1173	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	8	26	from	content	1072:1078	arg1	leblebi					1187:1193	roasted leblebi	1179:1193	roasted leblebi	1179:1193	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	3	27	theme	view	367:370	arg1	point					358:362	the consumers' point	343:362	the consumers' point of view	343:370	From the consumers' point of view, it is desirable to improve texture and palatability of the chickpea by the processing steps used to make leblebi.
26374493	8	28	theme	estimated	1107:1115	arg1	index					1126:1130	low estimated glycemic index	1103:1130	low estimated glycemic index (38.67 to 41.28)	1103:1147	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	8	28	theme	estimated	1107:1115	arg1	41.28					1142:1146	41.28	1142:1146	41.28	1142:1146	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	8	29	theme	low	1103:1105	arg1	index					1126:1130	low estimated glycemic index	1103:1130	low estimated glycemic index (38.67 to 41.28)	1103:1147	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	8	29	theme	low	1103:1105	arg1	41.28					1142:1146	41.28	1142:1146	41.28	1142:1146	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	10	30	theme	glycemic	1399:1406	arg1	response					1408:1415	glycemic response	1399:1415	glycemic response	1399:1415	White leblebi had relatively high sodium content and glycemic response.
26374493	6	31	theme	starch	759:764	arg1	damage					766:771	starch damage	759:771	starch damage	759:771	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	8	32	theme	High	1050:1053	arg1	starch					1065:1070	High resistant starch	1050:1070	High resistant starch content (28.28% to 30.20%)	1050:1097	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	1	33	theme	nutritional	173:183	arg1	properties					185:194	its nutritional properties	169:194	its nutritional properties	169:194	BACKGROUND Chickpea is considered a wholesome and nutritious food due to its nutritional properties and glycemic response.
26374493	0	34	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, nutritional value and in vitro starch digestibility of roasted chickpeas.
26374493	9	35	dep	CONCLUSION	1210:1219	arg1	indicate					1233:1240	indicate	1233:1240	indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response	1233:1343	CONCLUSION The results indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response.
26374493	5	36	theme	digestibility	611:623	arg1	properties					625:634	digestibility properties	611:634	digestibility properties	611:634	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	10	37	theme	White	1346:1350	arg1	leblebi					1352:1358	White leblebi	1346:1358	White leblebi	1346:1358	White leblebi had relatively high sodium content and glycemic response.
26374493	7	38	theme	samples	1041:1047	arg1	properties					1023:1032	the viscosity and starch properties	998:1032	the viscosity and starch properties of the samples	998:1047	Heat treatment and processing significantly (P < 0.05) altered the viscosity and starch properties of the samples.
26374493	7	39	theme	starch	1016:1021	arg1	properties					1023:1032	the viscosity and starch properties	998:1032	the viscosity and starch properties of the samples	998:1047	Heat treatment and processing significantly (P < 0.05) altered the viscosity and starch properties of the samples.
26374493	8	40	theme	heat-treated	1152:1163	arg1	chickpeas					1165:1173	heat-treated chickpeas	1152:1173	heat-treated chickpeas	1152:1173	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	0	41	theme	nutritional	22:32	arg1	value					34:38	nutritional value	22:38	nutritional value	22:38	Chemical composition, nutritional value and in vitro starch digestibility of roasted chickpeas.
26374493	5	42	theme	single	680:685	arg1	leblebi					706:712	single and double roasted leblebi	680:712	single and double roasted leblebi	680:712	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	1	43	theme	BACKGROUND	96:105	arg1	Chickpea					107:114	BACKGROUND Chickpea	96:114	BACKGROUND Chickpea	96:114	BACKGROUND Chickpea is considered a wholesome and nutritious food due to its nutritional properties and glycemic response.
26374493	8	44	theme	roasted	1179:1185	arg1	leblebi					1187:1193	roasted leblebi	1179:1193	roasted leblebi	1179:1193	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	8	45	theme	glycemic	1117:1124	arg1	index					1126:1130	low estimated glycemic index	1103:1130	low estimated glycemic index (38.67 to 41.28)	1103:1147	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	8	45	theme	glycemic	1117:1124	arg1	41.28					1142:1146	41.28	1142:1146	41.28	1142:1146	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	6	46	theme	fiber	920:924	arg1	content					926:932	significantly (P < 0.05) higher insoluble dietary fiber content	870:932	significantly (P < 0.05) higher insoluble dietary fiber content	870:932	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	3	47	theme	chickpea	432:439	arg1	texture					400:406	texture	400:406	texture	400:406	From the consumers' point of view, it is desirable to improve texture and palatability of the chickpea by the processing steps used to make leblebi.
26374493	3	47	theme	chickpea	432:439	arg1	palatability					412:423	palatability	412:423	palatability	412:423	From the consumers' point of view, it is desirable to improve texture and palatability of the chickpea by the processing steps used to make leblebi.
26374493	6	48	theme	dietary	912:918	arg1	content					926:932	significantly (P < 0.05) higher insoluble dietary fiber content	870:932	significantly (P < 0.05) higher insoluble dietary fiber content	870:932	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	5	49	theme	double	691:696	arg1	leblebi					706:712	single and double roasted leblebi	680:712	single and double roasted leblebi	680:712	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	9	50	theme	roasted	1273:1279	arg1	leblebi					1281:1287	roasted leblebi	1273:1287	roasted leblebi	1273:1287	CONCLUSION The results indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response.
26374493	6	51	theme	insoluble	902:910	arg1	content					926:932	significantly (P < 0.05) higher insoluble dietary fiber content	870:932	significantly (P < 0.05) higher insoluble dietary fiber content	870:932	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	5	52	theme	roasted	698:704	arg1	leblebi					706:712	single and double roasted leblebi	680:712	single and double roasted leblebi	680:712	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	0	53	dep	in	44:45	arg1	vitro					47:51	vitro	47:51	vitro	47:51	Chemical composition, nutritional value and in vitro starch digestibility of roasted chickpeas.
26374493	6	54	theme	High	746:749	arg1	sodium					751:756	High sodium	746:756	High sodium	746:756	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	6	55	located	observed	812:819	arg1	leblebi					830:836	white leblebi	824:836	white leblebi	824:836	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	6	55	located	observed	812:819	arg2	damage					766:771	starch damage	759:771	starch damage	759:771	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	6	55	located	observed	812:819	arg2	content					799:805	soluble dietary fiber content	777:805	soluble dietary fiber content	777:805	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	6	55	located	observed	812:819	arg2	sodium					751:756	High sodium	746:756	High sodium	746:756	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	6	56	theme	higher	895:900	arg1	content					926:932	significantly (P < 0.05) higher insoluble dietary fiber content	870:932	significantly (P < 0.05) higher insoluble dietary fiber content	870:932	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	5	57	theme	double	650:655	arg1	chickpea					670:677	single and double heat-treated chickpea	639:677	single and double heat-treated chickpea	639:677	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	6	58	theme	white	824:828	arg1	leblebi					830:836	white leblebi	824:836	white leblebi	824:836	High sodium, starch damage and soluble dietary fiber content were observed in white leblebi; while the other samples showed significantly (P < 0.05) higher insoluble dietary fiber content.
26374493	3	59	theme	processing	448:457	arg1	steps					459:463	the processing steps	444:463	the processing steps used to make leblebi	444:484	From the consumers' point of view, it is desirable to improve texture and palatability of the chickpea by the processing steps used to make leblebi.
26374493	0	60	theme	in	44:45	arg1	digestibility					60:72	in vitro starch digestibility	44:72	in vitro starch digestibility	44:72	Chemical composition, nutritional value and in vitro starch digestibility of roasted chickpeas.
26374493	9	61	theme	nutritional	1299:1309	arg1	quality					1311:1317	good nutritional quality	1294:1317	good nutritional quality	1294:1317	CONCLUSION The results indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response.
26374493	5	62	theme	heat-treated	657:668	arg1	chickpea					670:677	single and double heat-treated chickpea	639:677	single and double heat-treated chickpea	639:677	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	2	63	theme	Such	219:222	arg1	properties					224:233	Such properties	219:233	Such properties	219:233	Such properties can be influenced by the thermal treatment used to cook this legume and produce a snack named leblebi.
26374493	2	64	theme	named	323:327	arg1	leblebi					329:335	a snack named leblebi	315:335	a snack named leblebi	315:335	Such properties can be influenced by the thermal treatment used to cook this legume and produce a snack named leblebi.
26374493	5	65	theme	chickpea	670:677	arg1	properties					625:634	digestibility properties	611:634	digestibility properties	611:634	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	5	65	theme	chickpea	670:677	arg1	RESULTS					576:582	RESULTS Nutritional components and digestibility properties	576:634	RESULTS	576:582	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	2	66	theme	thermal	260:266	arg1	treatment					268:276	the thermal treatment	256:276	the thermal treatment used to cook this legume and produce a snack named leblebi	256:335	Such properties can be influenced by the thermal treatment used to cook this legume and produce a snack named leblebi.
26374493	5	67	dep	RESULTS	576:582	arg1	components					596:605	Nutritional components	584:605	Nutritional components	584:605	RESULTS Nutritional components and digestibility properties of single and double heat-treated chickpea, single and double roasted leblebi and white leblebi were studied.
26374493	4	68	theme	foods	569:573	arg1	value					554:558	the nutritional value	538:558	the nutritional value of snack foods	538:573	However, consumers are increasingly concerned with the nutritional value of snack foods.
26374493	8	69	theme	starch	1065:1070	arg1	%					1096:1096	28.28% to 30.20%	1081:1096	28.28% to 30.20%	1081:1096	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	8	69	theme	starch	1065:1070	arg1	content					1072:1078	High resistant starch content	1050:1078	High resistant starch content (28.28% to 30.20%)	1050:1097	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	9	70	theme	low	1323:1325	arg1	response					1336:1343	low glycemic response	1323:1343	low glycemic response	1323:1343	CONCLUSION The results indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response.
26374493	7	71	theme	P	980:980	arg1	<					982:982	P < 0.05	980:987	P < 0.05	980:987	Heat treatment and processing significantly (P < 0.05) altered the viscosity and starch properties of the samples.
26374493	1	72	theme	wholesome	132:140	arg1	food					157:160	a wholesome and nutritious food	130:160	a wholesome and nutritious food due to its nutritional properties and glycemic response	130:216	BACKGROUND Chickpea is considered a wholesome and nutritious food due to its nutritional properties and glycemic response.
26374493	4	73	theme	snack	563:567	arg1	foods					569:573	snack foods	563:573	snack foods	563:573	However, consumers are increasingly concerned with the nutritional value of snack foods.
26374493	9	74	theme	heat-treated	1247:1258	arg1	chickpea					1260:1267	heat-treated chickpea	1247:1267	heat-treated chickpea	1247:1267	CONCLUSION The results indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response.
26374493	9	75	theme	glycemic	1327:1334	arg1	response					1336:1343	low glycemic response	1323:1343	low glycemic response	1323:1343	CONCLUSION The results indicate that heat-treated chickpea and roasted leblebi have good nutritional quality and low glycemic response.
26374493	0	76	theme	starch	53:58	arg1	digestibility					60:72	in vitro starch digestibility	44:72	in vitro starch digestibility	44:72	Chemical composition, nutritional value and in vitro starch digestibility of roasted chickpeas.
26374493	8	77	from	index	1126:1130	arg1	chickpeas					1165:1173	heat-treated chickpeas	1152:1173	heat-treated chickpeas	1152:1173	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	8	77	from	index	1126:1130	arg1	leblebi					1187:1193	roasted leblebi	1179:1193	roasted leblebi	1179:1193	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
26374493	7	78	theme	viscosity	1002:1010	arg1	properties					1023:1032	the viscosity and starch properties	998:1032	the viscosity and starch properties of the samples	998:1047	Heat treatment and processing significantly (P < 0.05) altered the viscosity and starch properties of the samples.
26374493	7	79	theme	Heat	935:938	arg1	treatment					940:948	Heat treatment	935:948	Heat treatment	935:948	Heat treatment and processing significantly (P < 0.05) altered the viscosity and starch properties of the samples.
26374493	1	80	theme	nutritious	146:155	arg1	food					157:160	a wholesome and nutritious food	130:160	a wholesome and nutritious food due to its nutritional properties and glycemic response	130:216	BACKGROUND Chickpea is considered a wholesome and nutritious food due to its nutritional properties and glycemic response.
26374493	10	81	theme	high	1375:1378	arg1	content					1387:1393	relatively high sodium content	1364:1393	relatively high sodium content	1364:1393	White leblebi had relatively high sodium content and glycemic response.
26374493	8	82	dep	41.28	1142:1146	arg1	to					1139:1140	to	1139:1140	to	1139:1140	High resistant starch content (28.28% to 30.20%) and low estimated glycemic index (38.67 to 41.28) in heat-treated chickpeas and roasted leblebi were observed.
25356914	3	0	from	production	619:628	arg1	hydrolysate					654:664	the hydrolysate	650:664	the hydrolysate of pretreated straw	650:684	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b has been investigated.
25356914	6	1	theme	sugar	1219:1223	arg1	g/L					1203:1205	only 1.6 g/L	1194:1205	only 1.6 g/L of reducing sugar	1194:1223	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	2	2	theme	optimal	545:551	arg1	capacity					565:572	the optimal ventilatory capacity	541:572	the optimal ventilatory capacity	541:572	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	2	2	theme	optimal	545:551	arg1	medium					591:596	10.0 L/min/L medium	578:596	10.0 L/min/L medium	578:596	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	5	3	theme	cultivation	1017:1027	arg1	time					1029:1032	the cultivation time	1013:1032	the cultivation time	1013:1032	In the three-cycle fed-batch cultivation, citric acid (CA) production was increased to 42.4 g/L and the cultivation time was extended to 240 hr.
25356914	2	4	theme	natural	348:354	arg1	components					356:365	natural components	348:365	natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+)	348:463	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	2	5	theme	acid	490:493	arg1	production					495:504	citric acid production	483:504	citric acid production by Yarrowia lipolytica SWJ-1b	483:534	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	3	6	theme	citric	633:638	arg1	acid					640:643	citric acid	633:643	citric acid from the hydrolysate of pretreated straw	633:684	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b has been investigated.
25356914	1	7	theme	crude	154:158	arg1	cellulase					160:168	crude cellulase	154:168	crude cellulase produced by Trichoderma reesei Rut-30	154:206	In this study, crude cellulase produced by Trichoderma reesei Rut-30 was used to hydrolyze pretreated straw.
25356914	3	8	theme	acid	640:643	arg1	production					619:628	Batch and fed-batch production	599:628	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b	599:714	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b has been investigated.
25356914	6	9	theme	cultivation	1272:1282	arg1	end					1255:1257	the end	1251:1257	the end of fed-batch cultivation	1251:1282	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	3	10	theme	pretreated	669:678	arg1	straw					680:684	pretreated straw	669:684	pretreated straw	669:684	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b has been investigated.
25356914	6	11	theme	acid	1078:1081	arg1	yield					1089:1093	iso-citric acid (ICA) yield	1067:1093	iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L)	1067:1128	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	2	12	theme	hydrolysate	278:288	arg1	compositions					258:269	the compositions	254:269	the compositions of the hydrolysate of pretreated straw	254:308	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	6	13	from	medium	1241:1246	arg1	left					1229:1232	left	1229:1232	left	1229:1232	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	6	14	theme	batch	1161:1165	arg1	cultivation					1167:1177	the batch cultivation	1157:1177	the batch cultivation (3.9 g/L)	1157:1187	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	6	14	theme	batch	1161:1165	arg1	g/L					1184:1186	3.9 g/L	1180:1186	3.9 g/L	1180:1186	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	3	15	theme	straw	680:684	arg1	hydrolysate					654:664	the hydrolysate	650:664	the hydrolysate of pretreated straw	650:684	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b has been investigated.
25356914	2	16	theme	10.0	578:581	arg1	L/min/L					583:589	L/min/L	583:589	L/min/L	583:589	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	2	17	theme	L/min/L	583:589	arg1	capacity					565:572	the optimal ventilatory capacity	541:572	the optimal ventilatory capacity	541:572	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	2	17	theme	L/min/L	583:589	arg1	medium					591:596	10.0 L/min/L medium	578:596	10.0 L/min/L medium	578:596	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	6	18	used	used	1330:1333	arg2	carbon					1319:1324	the added carbon	1309:1324	the added carbon	1309:1324	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	6	18	used	used	1330:1333	arg2	most					1301:1304	most	1301:1304	most	1301:1304	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	0	19	theme	batch	106:110	arg1	cultivation					126:136	batch and fed-batch cultivation	106:136	cultivation	126:136	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.
25356914	6	20	from	yield	1089:1093	arg1	cultivation					1108:1118	fed-batch cultivation	1098:1118	fed-batch cultivation (4.0 g/L)	1098:1128	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	6	20	from	yield	1089:1093	arg1	g/L					1125:1127	4.0 g/L	1121:1127	4.0 g/L	1121:1127	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	4	21	theme	26.7	778:781	arg1	g/L					783:785	g/L	783:785	g/L	783:785	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	0	22	theme	acid	7:10	arg1	production					12:21	Citric acid production	0:21	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.	0:137	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.
25356914	0	23	from	hydrolysate	28:38	arg1	production					12:21	Citric acid production	0:21	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.	0:137	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.
25356914	0	24	dep	lipolytica	82:91	arg1	SWJ-1b					93:98	SWJ-1b	93:98	Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation	73:136	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.
25356914	2	25	theme	straw	381:385	arg1	components					356:365	natural components	348:365	natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+)	348:463	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	0	26	theme	Citric	0:5	arg1	production					12:21	Citric acid production	0:21	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.	0:137	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.
25356914	3	27	theme	lipolytica	698:707	arg1	SWJ-1b					709:714	Yarrowia lipolytica SWJ-1b	689:714	Yarrowia lipolytica SWJ-1b	689:714	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b has been investigated.
25356914	2	28	theme	ventilatory	553:563	arg1	capacity					565:572	the optimal ventilatory capacity	541:572	the optimal ventilatory capacity	541:572	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	2	28	theme	ventilatory	553:563	arg1	medium					591:596	10.0 L/min/L medium	578:596	10.0 L/min/L medium	578:596	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	4	29	theme	g/L	783:785	arg1	acid					794:797	26.7 g/L citric acid	778:797	26.7 g/L citric acid	778:797	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	1	30	theme	Trichoderma	182:192	arg1	reesei					194:199	Trichoderma reesei Rut-30	182:206	Trichoderma reesei Rut-30	182:206	In this study, crude cellulase produced by Trichoderma reesei Rut-30 was used to hydrolyze pretreated straw.
25356914	6	31	from	left	1229:1232	arg1	medium					1241:1246	the medium	1237:1246	the medium	1237:1246	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	6	32	theme	fed-batch	1098:1106	arg1	cultivation					1108:1118	fed-batch cultivation	1098:1118	fed-batch cultivation (4.0 g/L)	1098:1128	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	6	32	theme	fed-batch	1098:1106	arg1	g/L					1125:1127	4.0 g/L	1121:1127	4.0 g/L	1121:1127	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	2	33	theme	straw	304:308	arg1	hydrolysate					278:288	the hydrolysate	274:288	the hydrolysate of pretreated straw	274:308	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	2	34	theme	components	417:426	arg1	addition					395:402	addition	395:402	addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+)	395:463	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	6	35	theme	fed-batch	1262:1270	arg1	cultivation					1272:1282	fed-batch cultivation	1262:1282	fed-batch cultivation	1262:1282	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	0	36	theme	fed-batch	116:124	arg1	cultivation					126:136	batch and fed-batch cultivation	106:136	cultivation	126:136	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.
25356914	6	37	from	end	1255:1257	arg1	left					1229:1232	left	1229:1232	left	1229:1232	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	2	38	theme	pretreated	293:302	arg1	straw					304:308	pretreated straw	293:308	pretreated straw	293:308	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	2	39	theme	other	411:415	arg1	Mg					458:459	Mg	458:459	Mg(2+)	458:463	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	2	39	theme	other	411:415	arg1	KH2PO4					447:452	KH2PO4	447:452	KH2PO4	447:452	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	2	39	theme	other	411:415	arg1	NH4					437:439	(NH4)2SO4	436:444	(NH4)2SO4	436:444	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	2	39	theme	other	411:415	arg1	components					417:426	any other components	407:426	any other components such as (NH4)2SO4, KH2PO4, or Mg(2+)	407:463	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	2	40	theme	citric	483:488	arg1	production					495:504	citric acid production	483:504	citric acid production by Yarrowia lipolytica SWJ-1b	483:534	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	3	41	theme	Batch	599:603	arg1	production					619:628	Batch and fed-batch production	599:628	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b	599:714	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b has been investigated.
25356914	1	42	used	used	212:215	arg2	cellulase					160:168	crude cellulase	154:168	crude cellulase produced by Trichoderma reesei Rut-30	154:206	In this study, crude cellulase produced by Trichoderma reesei Rut-30 was used to hydrolyze pretreated straw.
25356914	2	43	theme	pretreated	370:379	arg1	straw					381:385	pretreated straw	370:385	pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+)	370:463	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	4	44	theme	cultivation	884:894	arg1	time					896:899	the cultivation time	880:899	the cultivation time	880:899	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	4	44	theme	cultivation	884:894	arg1	hr					909:910	120 hr	905:910	120 hr	905:910	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	0	45	theme	straw	54:58	arg1	cellulose					60:68	pretreated straw cellulose	43:68	pretreated straw cellulose	43:68	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.
25356914	5	46	theme	citric	955:960	arg1	CA					968:969	CA	968:969	CA	968:969	In the three-cycle fed-batch cultivation, citric acid (CA) production was increased to 42.4 g/L and the cultivation time was extended to 240 hr.
25356914	5	46	theme	citric	955:960	arg1	acid					962:965	citric acid	955:965	citric acid (CA) production	955:981	In the three-cycle fed-batch cultivation, citric acid (CA) production was increased to 42.4 g/L and the cultivation time was extended to 240 hr.
25356914	4	47	theme	citric	787:792	arg1	acid					794:797	26.7 g/L citric acid	778:797	26.7 g/L citric acid	778:797	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	4	48	from	yields	804:809	arg1	cultivation					752:762	the batch cultivation	742:762	the batch cultivation	742:762	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	0	49	theme	pretreated	43:52	arg1	cellulose					60:68	pretreated straw cellulose	43:68	pretreated straw cellulose	43:68	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.
25356914	2	50	theme	lipolytica	518:527	arg1	SWJ-1b					529:534	Yarrowia lipolytica SWJ-1b	509:534	Yarrowia lipolytica SWJ-1b	509:534	After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium.
25356914	5	51	theme	acid	962:965	arg1	production					972:981	citric acid (CA) production	955:981	citric acid (CA) production	955:981	In the three-cycle fed-batch cultivation, citric acid (CA) production was increased to 42.4 g/L and the cultivation time was extended to 240 hr.
25356914	6	52	theme	added	1313:1317	arg1	carbon					1319:1324	the added carbon	1309:1324	the added carbon	1309:1324	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	5	53	theme	fed-batch	932:940	arg1	cultivation					942:952	the three-cycle fed-batch cultivation	916:952	the three-cycle fed-batch cultivation	916:952	In the three-cycle fed-batch cultivation, citric acid (CA) production was increased to 42.4 g/L and the cultivation time was extended to 240 hr.
25356914	4	54	theme	straw	843:847	arg1	yields					804:809	yields	804:809	yields from glucose	804:822	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	4	54	theme	straw	843:847	arg1	hydrolysate					828:838	hydrolysate	828:838	hydrolysate of straw	828:847	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	6	55	theme	reducing	1210:1217	arg1	sugar					1219:1223	reducing sugar	1210:1223	reducing sugar	1210:1223	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	4	56	from	hydrolysate	828:838	arg1	cultivation					752:762	the batch cultivation	742:762	the batch cultivation	742:762	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	3	57	theme	fed-batch	609:617	arg1	production					619:628	Batch and fed-batch production	599:628	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b	599:714	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b has been investigated.
25356914	5	58	theme	three-cycle	920:930	arg1	cultivation					942:952	the three-cycle fed-batch cultivation	916:952	the three-cycle fed-batch cultivation	916:952	In the three-cycle fed-batch cultivation, citric acid (CA) production was increased to 42.4 g/L and the cultivation time was extended to 240 hr.
25356914	6	59	theme	iso-citric	1067:1076	arg1	ICA					1084:1086	ICA	1084:1086	ICA	1084:1086	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	6	59	theme	iso-citric	1067:1076	arg1	acid					1078:1081	iso-citric acid	1067:1081	iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L)	1067:1128	However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.
25356914	1	60	theme	pretreated	230:239	arg1	straw					241:245	pretreated straw	230:245	pretreated straw	230:245	In this study, crude cellulase produced by Trichoderma reesei Rut-30 was used to hydrolyze pretreated straw.
25356914	0	61	theme	cellulose	60:68	arg1	hydrolysate					28:38	hydrolysate	28:38	hydrolysate of pretreated straw cellulose	28:68	Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.
25356914	4	62	from	glucose	816:822	arg1	yields					804:809	yields	804:809	yields from glucose	804:822	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	4	62	from	glucose	816:822	arg1	hydrolysate					828:838	hydrolysate	828:838	hydrolysate of straw	828:847	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	4	63	theme	batch	746:750	arg1	cultivation					752:762	the batch cultivation	742:762	the batch cultivation	742:762	In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr.
25356914	3	64	from	hydrolysate	654:664	arg1	production					619:628	Batch and fed-batch production	599:628	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b	599:714	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b has been investigated.
25356914	3	64	from	hydrolysate	654:664	arg1	acid					640:643	citric acid	633:643	citric acid from the hydrolysate of pretreated straw	633:684	Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b has been investigated.
27671019	1	0	from	acid	317:320	arg1	properties					210:219	antioxidant and antibacterial properties	180:219	antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA)	180:325	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	5	1	theme	PEC	843:845	arg1	mats					862:865	the PEC QCh/Car-coated mats	839:865	the PEC QCh/Car-coated mats	839:865	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	1	2	theme	antibacterial	196:208	arg1	properties					210:219	antioxidant and antibacterial properties	180:219	antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA)	180:325	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	4	3	theme	diffraction	570:580	arg1	XRD					592:594	XRD	592:594	XRD	592:594	X-ray diffraction analysis (XRD) and differential scanning calorimetry (DSC) revealed that CA incorporated in the fibers was in the amorphous state, whereas CA included in the coating was in the crystalline state.
27671019	4	3	theme	diffraction	570:580	arg1	analysis					582:589	X-ray diffraction analysis	564:589	X-ray diffraction analysis (XRD)	564:595	X-ray diffraction analysis (XRD) and differential scanning calorimetry (DSC) revealed that CA incorporated in the fibers was in the amorphous state, whereas CA included in the coating was in the crystalline state.
27671019	5	4	located	found	872:876	arg2	mats					830:833	the CA-containing mats	812:833	the CA-containing mats	812:833	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	5	4	located	found	872:876	arg2	effective					971:979	effective	971:979	effective	971:979	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	5	4	located	found	872:876	arg1	contrast					781:788	contrast	781:788	contrast to the neat PHB mats	781:809	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	5	4	located	found	872:876	arg2	mats					862:865	the PEC QCh/Car-coated mats	839:865	the PEC QCh/Car-coated mats	839:865	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	5	5	theme	S.	913:914	arg1	bacteria					904:911	the Gram-positive bacteria	886:911	the Gram-positive bacteria S. aureus	886:921	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	6	6	theme	fibrous	1090:1096	arg1	materials					1098:1106	the fibrous materials	1086:1106	the fibrous materials containing both CA and QCh/Car coating	1086:1145	Enhancement of the antioxidant activity of the fibrous materials containing both CA and QCh/Car coating was observed.
27671019	6	7	contain	containing	1108:1117	arg2	QCh/Car					1131:1137	QCh/Car	1131:1137	QCh/Car	1131:1137	Enhancement of the antioxidant activity of the fibrous materials containing both CA and QCh/Car coating was observed.
27671019	6	7	contain	containing	1108:1117	arg1	materials					1098:1106	the fibrous materials	1086:1106	the fibrous materials containing both CA and QCh/Car coating	1086:1145	Enhancement of the antioxidant activity of the fibrous materials containing both CA and QCh/Car coating was observed.
27671019	6	7	contain	containing	1108:1117	arg2	CA					1124:1125	CA	1124:1125	CA	1124:1125	Enhancement of the antioxidant activity of the fibrous materials containing both CA and QCh/Car coating was observed.
27671019	4	8	theme	amorphous	696:704	arg1	state					706:710	the amorphous state	692:710	the amorphous state	692:710	X-ray diffraction analysis (XRD) and differential scanning calorimetry (DSC) revealed that CA incorporated in the fibers was in the amorphous state, whereas CA included in the coating was in the crystalline state.
27671019	6	9	dep	CA	1124:1125	arg1	coating					1139:1145	coating	1139:1145	coating	1139:1145	Enhancement of the antioxidant activity of the fibrous materials containing both CA and QCh/Car coating was observed.
27671019	0	10	theme	antioxidant	129:139	arg1	materials					87:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials	0:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.	0:149	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	10	theme	antioxidant	129:139	arg1	activity					141:148	antioxidant activity	129:148	antioxidant activity	129:148	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	2	11	with	conjunction	422:432	arg1	formation					485:493	polyelectrolyte complex (PEC) formation	455:493	polyelectrolyte complex (PEC) formation	455:493	These materials were prepared by applying electrospinning or electrospinning in conjunction with dip-coating and polyelectrolyte complex (PEC) formation.
27671019	2	11	with	conjunction	422:432	arg1	dip-coating					439:449	dip-coating	439:449	dip-coating	439:449	These materials were prepared by applying electrospinning or electrospinning in conjunction with dip-coating and polyelectrolyte complex (PEC) formation.
27671019	1	12	with	materials	165:173	arg1	properties					210:219	antioxidant and antibacterial properties	180:219	antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA)	180:325	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	2	13	theme	polyelectrolyte	455:469	arg1	formation					485:493	polyelectrolyte complex (PEC) formation	455:493	polyelectrolyte complex (PEC) formation	455:493	These materials were prepared by applying electrospinning or electrospinning in conjunction with dip-coating and polyelectrolyte complex (PEC) formation.
27671019	3	14	theme	CA	518:519	arg1	release					521:527	the CA release	514:527	the CA release	514:527	It was found that the CA release depended on the fiber composition.
27671019	1	15	from	κ-carrageenan	285:297	arg1	properties					210:219	antioxidant and antibacterial properties	180:219	antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA)	180:325	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	5	16	theme	CA-containing	816:828	arg1	effective					971:979	effective	971:979	effective	971:979	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	5	16	theme	CA-containing	816:828	arg1	mats					830:833	the CA-containing mats	812:833	the CA-containing mats	812:833	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	0	17	theme	Quaternized	0:10	arg1	Preparation					98:108	Preparation	98:108	Preparation	98:108	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	17	theme	Quaternized	0:10	arg1	materials					87:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials	0:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.	0:149	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	17	theme	Quaternized	0:10	arg1	antibacterial					111:123	antibacterial	111:123	antibacterial	111:123	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	17	theme	Quaternized	0:10	arg1	activity					141:148	antioxidant activity	129:148	antioxidant activity	129:148	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	5	18	theme	QCh/Car-coated	847:860	arg1	mats					862:865	the PEC QCh/Car-coated mats	839:865	the PEC QCh/Car-coated mats	839:865	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	3	19	theme	fiber	545:549	arg1	composition					551:561	the fiber composition	541:561	the fiber composition	541:561	It was found that the CA release depended on the fiber composition.
27671019	5	20	dep	E.	954:955	arg1	coli					957:960	coli	957:960	coli	957:960	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	0	21	theme	acid-coated	43:53	arg1	Preparation					98:108	Preparation	98:108	Preparation	98:108	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	21	theme	acid-coated	43:53	arg1	materials					87:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials	0:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.	0:149	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	21	theme	acid-coated	43:53	arg1	antibacterial					111:123	antibacterial	111:123	antibacterial	111:123	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	21	theme	acid-coated	43:53	arg1	activity					141:148	antioxidant activity	129:148	antioxidant activity	129:148	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	4	22	theme	X-ray	564:568	arg1	XRD					592:594	XRD	592:594	XRD	592:594	X-ray diffraction analysis (XRD) and differential scanning calorimetry (DSC) revealed that CA incorporated in the fibers was in the amorphous state, whereas CA included in the coating was in the crystalline state.
27671019	4	22	theme	X-ray	564:568	arg1	analysis					582:589	X-ray diffraction analysis	564:589	X-ray diffraction analysis (XRD)	564:595	X-ray diffraction analysis (XRD) and differential scanning calorimetry (DSC) revealed that CA incorporated in the fibers was in the amorphous state, whereas CA included in the coating was in the crystalline state.
27671019	5	23	theme	pathogenic	1012:1021	arg1	bacteria					1023:1030	pathogenic bacteria	1012:1030	pathogenic bacteria	1012:1030	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	0	24	dep	materials	87:95	arg1	Preparation					98:108	Preparation	98:108	Preparation	98:108	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	24	dep	materials	87:95	arg1	materials					87:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials	0:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.	0:149	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	24	dep	materials	87:95	arg1	antibacterial					111:123	antibacterial	111:123	antibacterial	111:123	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	24	dep	materials	87:95	arg1	activity					141:148	antioxidant activity	129:148	antioxidant activity	129:148	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	5	25	theme	neat	797:800	arg1	mats					806:809	the neat PHB mats	793:809	the neat PHB mats	793:809	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	5	26	theme	bacteria	1023:1030	arg1	adhesion					1000:1007	the adhesion	996:1007	the adhesion of pathogenic bacteria	996:1030	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	5	27	theme	PHB	802:804	arg1	mats					806:809	the neat PHB mats	793:809	the neat PHB mats	793:809	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	2	28	theme	PEC	480:482	arg1	formation					485:493	polyelectrolyte complex (PEC) formation	455:493	polyelectrolyte complex (PEC) formation	455:493	These materials were prepared by applying electrospinning or electrospinning in conjunction with dip-coating and polyelectrolyte complex (PEC) formation.
27671019	4	29	theme	scanning	614:621	arg1	DSC					636:638	DSC	636:638	DSC	636:638	X-ray diffraction analysis (XRD) and differential scanning calorimetry (DSC) revealed that CA incorporated in the fibers was in the amorphous state, whereas CA included in the coating was in the crystalline state.
27671019	4	29	theme	scanning	614:621	arg1	calorimetry					623:633	differential scanning calorimetry	601:633	differential scanning calorimetry (DSC)	601:639	X-ray diffraction analysis (XRD) and differential scanning calorimetry (DSC) revealed that CA incorporated in the fibers was in the amorphous state, whereas CA included in the coating was in the crystalline state.
27671019	0	30	theme	fibrous	79:85	arg1	Preparation					98:108	Preparation	98:108	Preparation	98:108	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	30	theme	fibrous	79:85	arg1	materials					87:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials	0:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.	0:149	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	30	theme	fibrous	79:85	arg1	antibacterial					111:123	antibacterial	111:123	antibacterial	111:123	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	30	theme	fibrous	79:85	arg1	activity					141:148	antioxidant activity	129:148	antioxidant activity	129:148	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	1	31	theme	caffeic	309:315	arg1	CA					323:324	CA	323:324	CA	323:324	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	1	31	theme	caffeic	309:315	arg1	acid					317:320	caffeic acid	309:320	caffeic acid (CA)	309:325	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	6	32	theme	activity	1074:1081	arg1	Enhancement					1043:1053	Enhancement	1043:1053	Enhancement of the antioxidant activity of the fibrous materials containing both CA and QCh/Car coating	1043:1145	Enhancement of the antioxidant activity of the fibrous materials containing both CA and QCh/Car coating was observed.
27671019	2	33	theme	complex	471:477	arg1	formation					485:493	polyelectrolyte complex (PEC) formation	455:493	polyelectrolyte complex (PEC) formation	455:493	These materials were prepared by applying electrospinning or electrospinning in conjunction with dip-coating and polyelectrolyte complex (PEC) formation.
27671019	1	34	from	chitosan	269:276	arg1	properties					210:219	antioxidant and antibacterial properties	180:219	antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA)	180:325	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	6	35	theme	materials	1098:1106	arg1	activity					1074:1081	the antioxidant activity	1058:1081	the antioxidant activity of the fibrous materials containing both CA and QCh/Car coating	1058:1145	Enhancement of the antioxidant activity of the fibrous materials containing both CA and QCh/Car coating was observed.
27671019	1	36	theme	Novel	151:155	arg1	materials					165:173	Novel fibrous materials	151:173	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA)	151:325	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	5	37	theme	Gram-negative	931:943	arg1	bacteria					945:952	the Gram-negative bacteria	927:952	the Gram-negative bacteria E. coli	927:960	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	1	38	theme	fibrous	157:163	arg1	materials					165:173	Novel fibrous materials	151:173	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA)	151:325	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	5	39	theme	Gram-positive	890:902	arg1	bacteria					904:911	the Gram-positive bacteria	886:911	the Gram-positive bacteria S. aureus	886:921	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	6	40	theme	antioxidant	1062:1072	arg1	activity					1074:1081	the antioxidant activity	1058:1081	the antioxidant activity of the fibrous materials containing both CA and QCh/Car coating	1058:1145	Enhancement of the antioxidant activity of the fibrous materials containing both CA and QCh/Car coating was observed.
27671019	1	41	from	poly	226:229	arg1	properties					210:219	antioxidant and antibacterial properties	180:219	antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA)	180:325	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	1	42	theme	antioxidant	180:190	arg1	properties					210:219	antioxidant and antibacterial properties	180:219	antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA)	180:325	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	5	43	dep	S.	913:914	arg1	aureus					916:921	aureus	916:921	aureus	916:921	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	5	44	theme	E.	954:955	arg1	bacteria					945:952	the Gram-negative bacteria	927:952	the Gram-negative bacteria E. coli	927:960	In contrast to the neat PHB mats, the CA-containing mats and the PEC QCh/Car-coated mats were found to kill the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli and were effective in suppressing the adhesion of pathogenic bacteria S. aureus.
27671019	1	45	theme	quaternized	257:267	arg1	QCh					279:281	QCh	279:281	QCh	279:281	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	1	45	theme	quaternized	257:267	arg1	chitosan					269:276	quaternized chitosan	257:276	quaternized chitosan (QCh)	257:282	Novel fibrous materials with antioxidant and antibacterial properties from poly(3-hydroxybutyrate) (PHB), quaternized chitosan (QCh), κ-carrageenan (Car) and caffeic acid (CA) were obtained.
27671019	4	46	theme	crystalline	759:769	arg1	state					771:775	the crystalline state	755:775	the crystalline state	755:775	X-ray diffraction analysis (XRD) and differential scanning calorimetry (DSC) revealed that CA incorporated in the fibers was in the amorphous state, whereas CA included in the coating was in the crystalline state.
27671019	0	47	theme	chitosan/κ-carrageenan/caffeic	12:41	arg1	Preparation					98:108	Preparation	98:108	Preparation	98:108	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	47	theme	chitosan/κ-carrageenan/caffeic	12:41	arg1	materials					87:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials	0:95	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.	0:149	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	47	theme	chitosan/κ-carrageenan/caffeic	12:41	arg1	antibacterial					111:123	antibacterial	111:123	antibacterial	111:123	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	0	47	theme	chitosan/κ-carrageenan/caffeic	12:41	arg1	activity					141:148	antioxidant activity	129:148	antioxidant activity	129:148	Quaternized chitosan/κ-carrageenan/caffeic acid-coated poly(3-hydroxybutyrate) fibrous materials: Preparation, antibacterial and antioxidant activity.
27671019	4	48	theme	differential	601:612	arg1	DSC					636:638	DSC	636:638	DSC	636:638	X-ray diffraction analysis (XRD) and differential scanning calorimetry (DSC) revealed that CA incorporated in the fibers was in the amorphous state, whereas CA included in the coating was in the crystalline state.
27671019	4	48	theme	differential	601:612	arg1	calorimetry					623:633	differential scanning calorimetry	601:633	differential scanning calorimetry (DSC)	601:639	X-ray diffraction analysis (XRD) and differential scanning calorimetry (DSC) revealed that CA incorporated in the fibers was in the amorphous state, whereas CA included in the coating was in the crystalline state.
28987583	6	0	theme	particle	1038:1045	arg1	size					1047:1050	particle size	1038:1050	particle size	1038:1050	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	2	1	theme	dairy	361:365	arg1	cattle					367:372	dairy cattle	361:372	dairy cattle	361:372	The objective of this work was to leverage equations derived in a meta-analysis into an ensemble modeling system for estimating dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle.
28987583	6	2	theme	fiber	1133:1137	arg1	system					1139:1144	a physically adjusted fiber system	1111:1144	a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets	1111:1263	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	2	3	theme	work	150:153	arg1	objective					132:140	The objective	128:140	The objective of this work	128:153	The objective of this work was to leverage equations derived in a meta-analysis into an ensemble modeling system for estimating dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle.
28987583	12	4	theme	particle	2467:2474	arg1	size					2476:2479	diet particle size	2462:2479	diet particle size	2462:2479	Results of this study agreed with described interrelationships between the chemical and physical form of diets fed to dairy cows and quantified the links between NDF intake, diet particle size, and ruminal pH. Feeding recommendations can be interpolated from tables and figures included in this work.
28987583	2	5	theme	chemical	277:284	arg1	characteristics					286:300	dietary physical and chemical characteristics	256:300	dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle	256:372	The objective of this work was to leverage equations derived in a meta-analysis into an ensemble modeling system for estimating dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle.
28987583	7	6	theme	particle	1344:1351	arg1	separator					1353:1361	the Penn State particle separator	1329:1361	the Penn State particle separator (PSPS)	1329:1368	Particle size measures of TMR were limited to those found with the Penn State particle separator (PSPS).
28987583	7	6	theme	particle	1344:1351	arg1	PSPS					1364:1367	PSPS	1364:1367	PSPS	1364:1367	Particle size measures of TMR were limited to those found with the Penn State particle separator (PSPS).
28987583	9	7	theme	adjusted	1725:1732	arg1	system					1740:1745	the physically adjusted fiber system	1710:1745	the physically adjusted fiber system	1710:1745	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	2	8	theme	physical	264:271	arg1	characteristics					286:300	dietary physical and chemical characteristics	256:300	dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle	256:372	The objective of this work was to leverage equations derived in a meta-analysis into an ensemble modeling system for estimating dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle.
28987583	8	9	theme	8-mm	1637:1640	arg1	sieve					1642:1646	the 8-mm sieve	1633:1646	the 8-mm sieve of a PSPS	1633:1656	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	7	10	theme	Penn	1333:1336	arg1	separator					1353:1361	the Penn State particle separator	1329:1361	the Penn State particle separator (PSPS)	1329:1368	Particle size measures of TMR were limited to those found with the Penn State particle separator (PSPS).
28987583	7	10	theme	Penn	1333:1336	arg1	PSPS					1364:1367	PSPS	1364:1367	PSPS	1364:1367	Particle size measures of TMR were limited to those found with the Penn State particle separator (PSPS).
28987583	9	11	theme	8-mm	1825:1828	arg1	sieves					1830:1835	8-mm sieves	1825:1835	8-mm sieves	1825:1835	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	6	12	theme	particle	1183:1190	arg1	sizes					1192:1196	minimum particle sizes	1175:1196	minimum particle sizes of TMR	1175:1203	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	3	13	theme	primary	551:557	arg1	indicator					577:585	the primary rumen environment indicator	547:585	the primary rumen environment indicator	547:585	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	3	13	theme	primary	551:557	arg1	pH					530:531	mean ruminal pH	517:531	mean ruminal pH	517:531	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	8	14	theme	PSPS	1653:1656	arg1	sieve					1642:1646	the 8-mm sieve	1633:1646	the 8-mm sieve of a PSPS	1633:1656	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	6	15	theme	minimum	1175:1181	arg1	sizes					1192:1196	minimum particle sizes	1175:1196	minimum particle sizes of TMR	1175:1203	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	9	16	theme	PSPS	1844:1847	arg1	size					1874:1877	estimated mean particle size	1850:1877	estimated mean particle size	1850:1877	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	16	theme	PSPS	1844:1847	arg1	19-					1817:1819	the 19-	1813:1819	the 19-	1813:1819	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	16	theme	PSPS	1844:1847	arg1	NDF					1981:1983	NDF	1981:1983	NDF	1981:1983	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	16	theme	PSPS	1844:1847	arg1	concentrations					1892:1905	the dietary concentrations	1880:1905	the dietary concentrations of forage, forage NDF, starch, and NDF	1880:1944	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	16	theme	PSPS	1844:1847	arg1	sieves					1830:1835	8-mm sieves	1825:1835	8-mm sieves	1825:1835	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	16	theme	PSPS	1844:1847	arg1	starch					1970:1975	ruminally degraded starch	1951:1975	ruminally degraded starch	1951:1975	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	2	17	theme	ensemble	216:223	arg1	system					234:239	an ensemble modeling system	213:239	an ensemble modeling system for estimating dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle	213:372	The objective of this work was to leverage equations derived in a meta-analysis into an ensemble modeling system for estimating dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle.
28987583	9	18	theme	estimated	1850:1858	arg1	size					1874:1877	estimated mean particle size	1850:1877	estimated mean particle size	1850:1877	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	6	19	theme	ruminal	1246:1252	arg1	targets					1257:1263	ruminal pH targets	1246:1263	ruminal pH targets	1246:1263	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	3	20	theme	environment	565:575	arg1	indicator					577:585	the primary rumen environment indicator	547:585	the primary rumen environment indicator	547:585	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	3	20	theme	environment	565:575	arg1	pH					530:531	mean ruminal pH	517:531	mean ruminal pH	517:531	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	5	21	theme	particle	868:875	arg1	size					877:880	a particle size	866:880	a particle size measure	866:888	The peNDF of feedstuffs is typically estimated by multiplying the NDF content by a particle size measure, resulting in an estimated index of effectiveness.
28987583	8	22	theme	specific	1385:1392	arg1	characteristics					1399:1413	specific diet characteristics	1385:1413	specific diet characteristics	1385:1413	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	7	23	theme	TMR	1292:1294	arg1	measures					1280:1287	Particle size measures	1266:1287	Particle size measures of TMR	1266:1294	Particle size measures of TMR were limited to those found with the Penn State particle separator (PSPS).
28987583	9	24	theme	particle	1865:1872	arg1	size					1874:1877	estimated mean particle size	1850:1877	estimated mean particle size	1850:1877	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	10	25	theme	material	2048:2055	arg1	proportion					2034:2043	the minimum proportion	2022:2043	the minimum proportion of material (dry matter basis) retained on the 8-mm sieve	2022:2101	The system was able to predict that the minimum proportion of material (dry matter basis) retained on the 8-mm sieve should increase with decreasing forage NDF or dietary NDF.
28987583	4	26	theme	detergent	661:669	arg1	fiber					671:675	neutral detergent fiber	653:675	neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen	653:782	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	4	26	theme	detergent	661:669	arg1	NDF					678:680	NDF	678:680	NDF	678:680	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	0	27	theme	dairy	65:69	arg1	rations					75:81	lactating dairy cow rations	55:81	lactating dairy cow rations	55:81	Physically adjusted neutral detergent fiber system for lactating dairy cow rations.
28987583	5	28	theme	NDF	851:853	arg1	content					855:861	the NDF content	847:861	the NDF content	847:861	The peNDF of feedstuffs is typically estimated by multiplying the NDF content by a particle size measure, resulting in an estimated index of effectiveness.
28987583	9	29	theme	continuous	1670:1679	arg1	variables					1681:1689	Additional continuous variables	1659:1689	Additional continuous variables also integrated in the physically adjusted fiber system	1659:1745	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	30	theme	dietary	1884:1890	arg1	concentrations					1892:1905	the dietary concentrations	1880:1905	the dietary concentrations of forage, forage NDF, starch, and NDF	1880:1944	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	4	31	from	mat	748:750	arg1	rumen					778:782	the rumen	774:782	the rumen	774:782	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	11	32	theme	material	2213:2220	arg1	proportion					2188:2197	the minimum proportion	2176:2197	the minimum proportion of dry matter material on the 8-mm sieve	2176:2238	Additionally, the minimum proportion of dry matter material on the 8-mm sieve should increase with increasing dietary starch.
28987583	5	33	dep	size	877:880	arg1	measure					882:888	measure	882:888	measure	882:888	The peNDF of feedstuffs is typically estimated by multiplying the NDF content by a particle size measure, resulting in an estimated index of effectiveness.
28987583	10	34	theme	dry	2058:2060	arg1	basis					2069:2073	dry matter basis	2058:2073	dry matter basis	2058:2073	The system was able to predict that the minimum proportion of material (dry matter basis) retained on the 8-mm sieve should increase with decreasing forage NDF or dietary NDF.
28987583	10	34	theme	dry	2058:2060	arg1	material					2048:2055	material	2048:2055	material	2048:2055	The system was able to predict that the minimum proportion of material (dry matter basis) retained on the 8-mm sieve should increase with decreasing forage NDF or dietary NDF.
28987583	3	35	theme	animal	439:444	arg1	behaviors					446:454	animal behaviors	439:454	animal behaviors	439:454	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	5	36	theme	effectiveness	926:938	arg1	index					917:921	an estimated index	904:921	an estimated index of effectiveness	904:938	The peNDF of feedstuffs is typically estimated by multiplying the NDF content by a particle size measure, resulting in an estimated index of effectiveness.
28987583	7	37	theme	size	1275:1278	arg1	measures					1280:1287	Particle size measures	1266:1287	Particle size measures of TMR	1266:1294	Particle size measures of TMR were limited to those found with the Penn State particle separator (PSPS).
28987583	8	38	theme	models	1451:1456	arg1	ensemble					1439:1446	an ensemble	1436:1446	an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS	1436:1656	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	9	39	theme	NDF	1925:1927	arg1	size					1874:1877	estimated mean particle size	1850:1877	estimated mean particle size	1850:1877	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	39	theme	NDF	1925:1927	arg1	19-					1817:1819	the 19-	1813:1819	the 19-	1813:1819	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	39	theme	NDF	1925:1927	arg1	NDF					1981:1983	NDF	1981:1983	NDF	1981:1983	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	39	theme	NDF	1925:1927	arg1	concentrations					1892:1905	the dietary concentrations	1880:1905	the dietary concentrations of forage, forage NDF, starch, and NDF	1880:1944	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	39	theme	NDF	1925:1927	arg1	sieves					1830:1835	8-mm sieves	1825:1835	8-mm sieves	1825:1835	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	39	theme	NDF	1925:1927	arg1	starch					1970:1975	ruminally degraded starch	1951:1975	ruminally degraded starch	1951:1975	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	3	40	theme	pH	433:434	arg1	responsiveness					407:420	responsiveness	407:420	responsiveness of ruminal pH to animal behaviors	407:454	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	3	40	theme	pH	433:434	arg1	composition					474:484	chemical composition	465:484	chemical composition	465:484	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	3	40	theme	pH	433:434	arg1	data					401:404	data	401:404	data	401:404	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	3	40	theme	pH	433:434	arg1	form					499:502	physical form	490:502	physical form	490:502	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	9	41	theme	fiber	1734:1738	arg1	system					1740:1745	the physically adjusted fiber system	1710:1745	the physically adjusted fiber system	1710:1745	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	42	theme	starch	1930:1935	arg1	size					1874:1877	estimated mean particle size	1850:1877	estimated mean particle size	1850:1877	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	42	theme	starch	1930:1935	arg1	19-					1817:1819	the 19-	1813:1819	the 19-	1813:1819	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	42	theme	starch	1930:1935	arg1	NDF					1981:1983	NDF	1981:1983	NDF	1981:1983	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	42	theme	starch	1930:1935	arg1	concentrations					1892:1905	the dietary concentrations	1880:1905	the dietary concentrations of forage, forage NDF, starch, and NDF	1880:1944	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	42	theme	starch	1930:1935	arg1	sieves					1830:1835	8-mm sieves	1825:1835	8-mm sieves	1825:1835	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	42	theme	starch	1930:1935	arg1	starch					1970:1975	ruminally degraded starch	1951:1975	ruminally degraded starch	1951:1975	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	0	43	theme	neutral	20:26	arg1	system					44:49	neutral detergent fiber system	20:49	neutral detergent fiber system	20:49	Physically adjusted neutral detergent fiber system for lactating dairy cow rations.
28987583	0	44	theme	fiber	38:42	arg1	system					44:49	neutral detergent fiber system	20:49	neutral detergent fiber system	20:49	Physically adjusted neutral detergent fiber system for lactating dairy cow rations.
28987583	8	45	theme	variable	1487:1494	arg1	mixture					1496:1502	a variable mixture	1485:1502	a variable mixture of experts	1485:1513	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	12	46	dep	Feeding	2498:2504	arg1	pH.					2494:2496	pH.	2494:2496	pH.	2494:2496	Results of this study agreed with described interrelationships between the chemical and physical form of diets fed to dairy cows and quantified the links between NDF intake, diet particle size, and ruminal pH. Feeding recommendations can be interpolated from tables and figures included in this work.
28987583	3	47	theme	physical	490:497	arg1	form					499:502	physical form	490:502	physical form	490:502	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	6	48	theme	dietary	1074:1080	arg1	all					1091:1093	all	1091:1093	all	1091:1093	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	6	48	theme	dietary	1074:1080	arg1	factors					1082:1088	other dietary factors	1068:1088	other dietary factors	1068:1088	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	9	49	theme	material	1773:1780	arg1	proportion					1759:1768	the proportion	1755:1768	the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF	1755:1983	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	4	50	theme	large	755:759	arg1	particles					761:769	large particles	755:769	large particles	755:769	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	3	51	theme	composition	474:484	arg1	availability					385:396	the availability	381:396	the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet	381:514	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	12	52	theme	diet	2462:2465	arg1	size					2476:2479	diet particle size	2462:2479	diet particle size	2462:2479	Results of this study agreed with described interrelationships between the chemical and physical form of diets fed to dairy cows and quantified the links between NDF intake, diet particle size, and ruminal pH. Feeding recommendations can be interpolated from tables and figures included in this work.
28987583	3	53	theme	ruminal	522:528	arg1	pH					530:531	mean ruminal pH	517:531	mean ruminal pH	517:531	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	3	53	theme	ruminal	522:528	arg1	indicator					577:585	the primary rumen environment indicator	547:585	the primary rumen environment indicator	547:585	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	12	54	theme	NDF	2450:2452	arg1	intake					2454:2459	NDF intake	2450:2459	NDF intake	2450:2459	Results of this study agreed with described interrelationships between the chemical and physical form of diets fed to dairy cows and quantified the links between NDF intake, diet particle size, and ruminal pH. Feeding recommendations can be interpolated from tables and figures included in this work.
28987583	9	55	theme	dry	1783:1785	arg1	material					1773:1780	material	1773:1780	material (dry matter basis)	1773:1799	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	55	theme	dry	1783:1785	arg1	basis					1794:1798	dry matter basis	1783:1798	dry matter basis	1783:1798	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	3	56	theme	diet	511:514	arg1	responsiveness					407:420	responsiveness	407:420	responsiveness of ruminal pH to animal behaviors	407:454	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	3	56	theme	diet	511:514	arg1	composition					474:484	chemical composition	465:484	chemical composition	465:484	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	3	56	theme	diet	511:514	arg1	data					401:404	data	401:404	data	401:404	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	3	56	theme	diet	511:514	arg1	form					499:502	physical form	490:502	physical form	490:502	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	8	57	theme	dietary	1586:1592	arg1	material					1597:1604	dietary DM material	1586:1604	dietary DM material	1586:1604	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	11	58	theme	minimum	2180:2186	arg1	proportion					2188:2197	the minimum proportion	2176:2197	the minimum proportion of dry matter material on the 8-mm sieve	2176:2238	Additionally, the minimum proportion of dry matter material on the 8-mm sieve should increase with increasing dietary starch.
28987583	8	59	theme	material	1597:1604	arg1	percentage					1572:1581	the percentage	1568:1581	the percentage of dietary DM material	1568:1604	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	12	60	theme	physical	2376:2383	arg1	form					2385:2388	the chemical and physical form	2359:2388	the chemical and physical form of diets fed to dairy cows	2359:2415	Results of this study agreed with described interrelationships between the chemical and physical form of diets fed to dairy cows and quantified the links between NDF intake, diet particle size, and ruminal pH. Feeding recommendations can be interpolated from tables and figures included in this work.
28987583	8	61	theme	robust	1541:1546	arg1	recommendations					1548:1562	more robust recommendations	1536:1562	more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS	1536:1656	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	6	62	theme	diet	1209:1212	arg1	compositions					1214:1225	diet compositions	1209:1225	diet compositions	1209:1225	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	9	63	dep	NDF	1925:1927	arg1	forage					1918:1923	forage	1918:1923	forage	1918:1923	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	6	64	theme	adjusted	1124:1131	arg1	system					1139:1144	a physically adjusted fiber system	1111:1144	a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets	1111:1263	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	6	65	theme	TMR	1201:1203	arg1	compositions					1214:1225	diet compositions	1209:1225	diet compositions	1209:1225	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	6	65	theme	TMR	1201:1203	arg1	sizes					1192:1196	minimum particle sizes	1175:1196	minimum particle sizes of TMR	1175:1203	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	3	66	theme	mean	517:520	arg1	pH					530:531	mean ruminal pH	517:531	mean ruminal pH	517:531	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	3	66	theme	mean	517:520	arg1	indicator					577:585	the primary rumen environment indicator	547:585	the primary rumen environment indicator	547:585	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	12	67	theme	dairy	2406:2410	arg1	cows					2412:2415	dairy cows	2406:2415	dairy cows	2406:2415	Results of this study agreed with described interrelationships between the chemical and physical form of diets fed to dairy cows and quantified the links between NDF intake, diet particle size, and ruminal pH. Feeding recommendations can be interpolated from tables and figures included in this work.
28987583	2	68	theme	desired	323:329	arg1	conditions					337:346	desired rumen conditions	323:346	desired rumen conditions	323:346	The objective of this work was to leverage equations derived in a meta-analysis into an ensemble modeling system for estimating dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle.
28987583	11	69	from	proportion	2188:2197	arg1	sieve					2234:2238	the 8-mm sieve	2225:2238	the 8-mm sieve	2225:2238	Additionally, the minimum proportion of dry matter material on the 8-mm sieve should increase with increasing dietary starch.
28987583	7	70	theme	State	1338:1342	arg1	separator					1353:1361	the Penn State particle separator	1329:1361	the Penn State particle separator (PSPS)	1329:1368	Particle size measures of TMR were limited to those found with the Penn State particle separator (PSPS).
28987583	7	70	theme	State	1338:1342	arg1	PSPS					1364:1367	PSPS	1364:1367	PSPS	1364:1367	Particle size measures of TMR were limited to those found with the Penn State particle separator (PSPS).
28987583	2	71	theme	modeling	225:232	arg1	system					234:239	an ensemble modeling system	213:239	an ensemble modeling system for estimating dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle	213:372	The objective of this work was to leverage equations derived in a meta-analysis into an ensemble modeling system for estimating dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle.
28987583	8	72	theme	a	1651:1651	arg1	PSPS					1653:1656	a PSPS	1651:1656	a PSPS	1651:1656	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	5	73	theme	feedstuffs	798:807	arg1	peNDF					789:793	The peNDF	785:793	The peNDF of feedstuffs	785:807	The peNDF of feedstuffs is typically estimated by multiplying the NDF content by a particle size measure, resulting in an estimated index of effectiveness.
28987583	10	74	theme	minimum	2026:2032	arg1	proportion					2034:2043	the minimum proportion	2022:2043	the minimum proportion of material (dry matter basis) retained on the 8-mm sieve	2022:2101	The system was able to predict that the minimum proportion of material (dry matter basis) retained on the 8-mm sieve should increase with decreasing forage NDF or dietary NDF.
28987583	6	75	theme	pH	1254:1255	arg1	targets					1257:1263	ruminal pH targets	1246:1263	ruminal pH targets	1246:1263	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	3	76	theme	rumen	559:563	arg1	indicator					577:585	the primary rumen environment indicator	547:585	the primary rumen environment indicator	547:585	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	3	76	theme	rumen	559:563	arg1	pH					530:531	mean ruminal pH	517:531	mean ruminal pH	517:531	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	1	77	theme	feeding	103:109	arg1	Development					88:98	II: Development	84:98	II: Development of feeding	84:109	II: Development of feeding recommendations.
28987583	12	78	theme	ruminal	2486:2492	arg1	recommendations					2506:2520	ruminal pH. Feeding recommendations	2486:2520	ruminal pH. Feeding recommendations	2486:2520	Results of this study agreed with described interrelationships between the chemical and physical form of diets fed to dairy cows and quantified the links between NDF intake, diet particle size, and ruminal pH. Feeding recommendations can be interpolated from tables and figures included in this work.
28987583	8	79	theme	diet	1394:1397	arg1	characteristics					1399:1413	specific diet characteristics	1385:1413	specific diet characteristics	1385:1413	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	9	80	theme	mean	1860:1863	arg1	size					1874:1877	estimated mean particle size	1850:1877	estimated mean particle size	1850:1877	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	2	81	theme	dietary	256:262	arg1	characteristics					286:300	dietary physical and chemical characteristics	256:300	dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle	256:372	The objective of this work was to leverage equations derived in a meta-analysis into an ensemble modeling system for estimating dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle.
28987583	6	82	theme	peNDF	977:981	arg1	utility					966:972	the utility	962:972	the utility of peNDF	962:981	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	12	83	theme	Feeding	2498:2504	arg1	recommendations					2506:2520	ruminal pH. Feeding recommendations	2486:2520	ruminal pH. Feeding recommendations	2486:2520	Results of this study agreed with described interrelationships between the chemical and physical form of diets fed to dairy cows and quantified the links between NDF intake, diet particle size, and ruminal pH. Feeding recommendations can be interpolated from tables and figures included in this work.
28987583	5	84	theme	estimated	907:915	arg1	index					917:921	an estimated index	904:921	an estimated index of effectiveness	904:938	The peNDF of feedstuffs is typically estimated by multiplying the NDF content by a particle size measure, resulting in an estimated index of effectiveness.
28987583	0	85	theme	cow	71:73	arg1	rations					75:81	lactating dairy cow rations	55:81	lactating dairy cow rations	55:81	Physically adjusted neutral detergent fiber system for lactating dairy cow rations.
28987583	9	86	theme	Additional	1659:1668	arg1	variables					1681:1689	Additional continuous variables	1659:1689	Additional continuous variables also integrated in the physically adjusted fiber system	1659:1745	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	11	87	theme	dietary	2272:2278	arg1	starch					2280:2285	dietary starch	2272:2285	dietary starch	2272:2285	Additionally, the minimum proportion of dry matter material on the 8-mm sieve should increase with increasing dietary starch.
28987583	4	88	theme	neutral	653:659	arg1	fiber					671:675	neutral detergent fiber	653:675	neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen	653:782	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	4	88	theme	neutral	653:659	arg1	NDF					678:680	NDF	678:680	NDF	678:680	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	11	89	theme	matter	2206:2211	arg1	material					2213:2220	dry matter material	2202:2220	dry matter material	2202:2220	Additionally, the minimum proportion of dry matter material on the 8-mm sieve should increase with increasing dietary starch.
28987583	4	90	theme	fiber	671:675	arg1	fiber					609:613	Physically effective fiber	588:613	Physically effective fiber (peNDF)	588:621	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	4	90	theme	fiber	671:675	arg1	fraction					641:648	the fraction	637:648	the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen	637:782	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	10	91	theme	matter	2062:2067	arg1	basis					2069:2073	dry matter basis	2058:2073	dry matter basis	2058:2073	The system was able to predict that the minimum proportion of material (dry matter basis) retained on the 8-mm sieve should increase with decreasing forage NDF or dietary NDF.
28987583	10	91	theme	matter	2062:2067	arg1	material					2048:2055	material	2048:2055	material	2048:2055	The system was able to predict that the minimum proportion of material (dry matter basis) retained on the 8-mm sieve should increase with decreasing forage NDF or dietary NDF.
28987583	3	92	dep	composition	474:484	arg1	the					461:463	the	461:463	the	461:463	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	9	93	theme	forage	1910:1915	arg1	size					1874:1877	estimated mean particle size	1850:1877	estimated mean particle size	1850:1877	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	93	theme	forage	1910:1915	arg1	19-					1817:1819	the 19-	1813:1819	the 19-	1813:1819	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	93	theme	forage	1910:1915	arg1	NDF					1981:1983	NDF	1981:1983	NDF	1981:1983	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	93	theme	forage	1910:1915	arg1	concentrations					1892:1905	the dietary concentrations	1880:1905	the dietary concentrations of forage, forage NDF, starch, and NDF	1880:1944	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	93	theme	forage	1910:1915	arg1	sieves					1830:1835	8-mm sieves	1825:1835	8-mm sieves	1825:1835	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	93	theme	forage	1910:1915	arg1	starch					1970:1975	ruminally degraded starch	1951:1975	ruminally degraded starch	1951:1975	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	7	94	theme	Particle	1266:1273	arg1	measures					1280:1287	Particle size measures	1266:1287	Particle size measures of TMR	1266:1294	Particle size measures of TMR were limited to those found with the Penn State particle separator (PSPS).
28987583	10	95	theme	8-mm	2092:2095	arg1	sieve					2097:2101	the 8-mm sieve	2088:2101	the 8-mm sieve	2088:2101	The system was able to predict that the minimum proportion of material (dry matter basis) retained on the 8-mm sieve should increase with decreasing forage NDF or dietary NDF.
28987583	4	96	theme	floating	739:746	arg1	mat					748:750	the floating mat	735:750	the floating mat of large particles in the rumen	735:782	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	3	97	theme	data	401:404	arg1	availability					385:396	the availability	381:396	the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet	381:514	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	4	98	theme	chewing	699:705	arg1	activity					707:714	chewing activity	699:714	chewing activity	699:714	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	0	99	theme	detergent	28:36	arg1	system					44:49	neutral detergent fiber system	20:49	neutral detergent fiber system	20:49	Physically adjusted neutral detergent fiber system for lactating dairy cow rations.
28987583	11	100	theme	dry	2202:2204	arg1	material					2213:2220	dry matter material	2202:2220	dry matter material	2202:2220	Additionally, the minimum proportion of dry matter material on the 8-mm sieve should increase with increasing dietary starch.
28987583	12	101	theme	study	2304:2308	arg1	Results					2288:2294	Results	2288:2294	Results of this study	2288:2308	Results of this study agreed with described interrelationships between the chemical and physical form of diets fed to dairy cows and quantified the links between NDF intake, diet particle size, and ruminal pH. Feeding recommendations can be interpolated from tables and figures included in this work.
28987583	3	102	theme	chemical	465:472	arg1	composition					474:484	chemical composition	465:484	chemical composition	465:484	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	12	103	theme	diets	2393:2397	arg1	form					2385:2388	the chemical and physical form	2359:2388	the chemical and physical form of diets fed to dairy cows	2359:2415	Results of this study agreed with described interrelationships between the chemical and physical form of diets fed to dairy cows and quantified the links between NDF intake, diet particle size, and ruminal pH. Feeding recommendations can be interpolated from tables and figures included in this work.
28987583	3	104	theme	ruminal	425:431	arg1	pH					433:434	ruminal pH	425:434	ruminal pH	425:434	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	9	105	theme	NDF	1942:1944	arg1	size					1874:1877	estimated mean particle size	1850:1877	estimated mean particle size	1850:1877	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	105	theme	NDF	1942:1944	arg1	19-					1817:1819	the 19-	1813:1819	the 19-	1813:1819	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	105	theme	NDF	1942:1944	arg1	NDF					1981:1983	NDF	1981:1983	NDF	1981:1983	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	105	theme	NDF	1942:1944	arg1	concentrations					1892:1905	the dietary concentrations	1880:1905	the dietary concentrations of forage, forage NDF, starch, and NDF	1880:1944	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	105	theme	NDF	1942:1944	arg1	sieves					1830:1835	8-mm sieves	1825:1835	8-mm sieves	1825:1835	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	105	theme	NDF	1942:1944	arg1	starch					1970:1975	ruminally degraded starch	1951:1975	ruminally degraded starch	1951:1975	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	0	106	theme	lactating	55:63	arg1	rations					75:81	lactating dairy cow rations	55:81	lactating dairy cow rations	55:81	Physically adjusted neutral detergent fiber system for lactating dairy cow rations.
28987583	3	107	theme	responsiveness	407:420	arg1	availability					385:396	the availability	381:396	the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet	381:514	Given the availability of data, responsiveness of ruminal pH to animal behaviors, and the chemical composition and physical form of the diet, mean ruminal pH was chosen as the primary rumen environment indicator.
28987583	8	108	theme	experts	1507:1513	arg1	mixture					1496:1502	a variable mixture	1485:1502	a variable mixture of experts	1485:1513	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	11	109	theme	8-mm	2229:2232	arg1	sieve					2234:2238	the 8-mm sieve	2225:2238	the 8-mm sieve	2225:2238	Additionally, the minimum proportion of dry matter material on the 8-mm sieve should increase with increasing dietary starch.
28987583	6	110	theme	other	1068:1072	arg1	all					1091:1093	all	1091:1093	all	1091:1093	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	6	110	theme	other	1068:1072	arg1	factors					1082:1088	other dietary factors	1068:1088	other dietary factors	1068:1088	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
28987583	4	111	theme	effective	599:607	arg1	fraction					641:648	the fraction	637:648	the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen	637:782	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	4	111	theme	effective	599:607	arg1	peNDF					616:620	peNDF	616:620	peNDF	616:620	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	4	111	theme	effective	599:607	arg1	fiber					609:613	Physically effective fiber	588:613	Physically effective fiber (peNDF)	588:621	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	9	112	theme	degraded	1961:1968	arg1	starch					1970:1975	ruminally degraded starch	1951:1975	ruminally degraded starch	1951:1975	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	2	113	theme	rumen	331:335	arg1	conditions					337:346	desired rumen conditions	323:346	desired rumen conditions	323:346	The objective of this work was to leverage equations derived in a meta-analysis into an ensemble modeling system for estimating dietary physical and chemical characteristics required to maintain desired rumen conditions in lactating dairy cattle.
28987583	8	114	theme	DM	1594:1595	arg1	material					1597:1604	dietary DM material	1586:1604	dietary DM material	1586:1604	Starting with specific diet characteristics, the system employed an ensemble of models that were integrated using a variable mixture of experts approach to generate more robust recommendations for the percentage of dietary DM material that should be retained on the 8-mm sieve of a PSPS.
28987583	12	115	theme	chemical	2363:2370	arg1	form					2385:2388	the chemical and physical form	2359:2388	the chemical and physical form of diets fed to dairy cows	2359:2415	Results of this study agreed with described interrelationships between the chemical and physical form of diets fed to dairy cows and quantified the links between NDF intake, diet particle size, and ruminal pH. Feeding recommendations can be interpolated from tables and figures included in this work.
28987583	9	116	theme	matter	1787:1792	arg1	material					1773:1780	material	1773:1780	material (dry matter basis)	1773:1799	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	9	116	theme	matter	1787:1792	arg1	basis					1794:1798	dry matter basis	1783:1798	dry matter basis	1783:1798	Additional continuous variables also integrated in the physically adjusted fiber system include the proportion of material (dry matter basis) retained on the 19- and 8-mm sieves of the PSPS, estimated mean particle size, the dietary concentrations of forage, forage NDF, starch, and NDF, and ruminally degraded starch and NDF.
28987583	4	117	theme	particles	761:769	arg1	mat					748:750	the floating mat	735:750	the floating mat of large particles in the rumen	735:782	Physically effective fiber (peNDF) is defined as the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen.
28987583	6	118	used	used	1158:1161	arg2	system					1139:1144	a physically adjusted fiber system	1111:1144	a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets	1111:1263	We hypothesized that the utility of peNDF could be expanded and improved by dissociating NDF and particle size and considering other dietary factors, all integrated into a physically adjusted fiber system that can be used to estimate minimum particle sizes of TMR and diet compositions needed to maintain ruminal pH targets.
25093631	6	0	theme	locking	1157:1163	arg1	change					1174:1179	a change	1172:1179	a change much greater than in surrounding tissues	1172:1220	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	6	0	theme	locking	1157:1163	arg1	field					1165:1169	the locking field	1153:1169	the locking field	1153:1169	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	3	1	theme	different	411:419	arg1	durations					431:439	5 different spin-lock durations	409:439	5 different spin-lock durations for each of 12 different spin-lock amplitudes	409:485	Quantitative estimates of R1ρ (=1/T1ρ) were made using 5 different spin-lock durations for each of 12 different spin-lock amplitudes over the range 0 to 550Hz.
25093631	5	2	from	3T	853:854	arg1	dispersion					805:814	in vivo R1ρ dispersion	793:814	in vivo R1ρ dispersion of human knee articular cartilage at 3T	793:854	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	9	3	theme	R1ρ	1508:1510	arg1	analysis					1523:1530	R1ρ dispersion analysis	1508:1530	CONCLUSION R1ρ dispersion analysis	1497:1530	CONCLUSION R1ρ dispersion analysis may provide more specific information for studying cartilage biochemical composition and form the basis for quantitative evaluation of cartilage disorders.
25093631	6	4	theme	articular	1070:1078	arg1	cartilage					1080:1088	articular cartilage	1070:1088	articular cartilage	1070:1088	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	7	5	theme	theoretical	1294:1304	arg1	model					1306:1310	the theoretical model	1290:1310	the theoretical model	1290:1310	From the theoretical model, the exchange rates in cartilage were estimated to be in the range of 1.0-3.0kHz, and varied within the tissue.
25093631	6	6	theme	high	1138:1141	arg1	values					1143:1148	low and high values	1130:1148	low and high values of the locking field, a change much greater than in surrounding tissues	1130:1220	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	3	7	dep	range	496:500	arg1	to					504:505	to	504:505	to	504:505	Quantitative estimates of R1ρ (=1/T1ρ) were made using 5 different spin-lock durations for each of 12 different spin-lock amplitudes over the range 0 to 550Hz.
25093631	4	8	theme	tissue	759:764	arg1	composition					766:776	tissue composition	759:776	tissue composition	759:776	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	0	9	theme	1/T1ρ	53:57	arg1	dispersion					60:69	R1ρ (1/T1ρ) dispersion	48:69	R1ρ (1/T1ρ) dispersion at 3T	48:75	Chemical exchange in knee cartilage assessed by R1ρ (1/T1ρ) dispersion at 3T.
25093631	6	10	theme	low	1130:1132	arg1	values					1143:1148	low and high values	1130:1148	low and high values of the locking field, a change much greater than in surrounding tissues	1130:1220	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	9	11	theme	specific	1549:1556	arg1	information					1558:1568	more specific information	1544:1568	more specific information for studying cartilage biochemical composition	1544:1615	CONCLUSION R1ρ dispersion analysis may provide more specific information for studying cartilage biochemical composition and form the basis for quantitative evaluation of cartilage disorders.
25093631	6	12	theme	R1ρ	1031:1033	arg1	values					1035:1040	R1ρ values	1031:1040	RESULTS R1ρ values	1023:1040	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	3	13	theme	spin-lock	466:474	arg1	amplitudes					476:485	12 different spin-lock amplitudes	453:485	12 different spin-lock amplitudes	453:485	Quantitative estimates of R1ρ (=1/T1ρ) were made using 5 different spin-lock durations for each of 12 different spin-lock amplitudes over the range 0 to 550Hz.
25093631	6	14	theme	greater	1186:1192	arg1	change					1174:1179	a change	1172:1179	a change much greater than in surrounding tissues	1172:1220	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	6	14	theme	greater	1186:1192	arg1	field					1165:1169	the locking field	1153:1169	the locking field	1153:1169	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	1	15	theme	dispersion	179:188	arg1	analysis					190:197	R1ρ dispersion analysis	175:197	R1ρ dispersion analysis	175:197	PURPOSE To quantify the characteristics of proton chemical exchange in knee cartilage in vivo by R1ρ dispersion analysis.
25093631	9	16	theme	cartilage	1583:1591	arg1	composition					1605:1615	cartilage biochemical composition	1583:1615	cartilage biochemical composition	1583:1615	CONCLUSION R1ρ dispersion analysis may provide more specific information for studying cartilage biochemical composition and form the basis for quantitative evaluation of cartilage disorders.
25093631	5	17	theme	macromolecular	922:935	arg1	hydroxyls					937:945	macromolecular hydroxyls	922:945	macromolecular hydroxyls (mainly in glycosaminoglycans)	922:976	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	4	18	theme	exchange	615:622	arg1	contributions					624:636	chemical exchange contributions	606:636	chemical exchange contributions	606:636	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	2	19	dep	71	287:288	arg1	to					284:285	to	284:285	to	284:285	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	2	20	theme	healthy	226:232	arg1	subjects					234:241	Six healthy subjects	222:241	Six healthy subjects	222:241	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	1	21	from	cartilage	154:162	arg1	characteristics					102:116	the characteristics	98:116	the characteristics of proton chemical exchange in knee cartilage	98:162	PURPOSE To quantify the characteristics of proton chemical exchange in knee cartilage in vivo by R1ρ dispersion analysis.
25093631	5	22	theme	human	819:823	arg1	cartilage					840:848	human knee articular cartilage	819:848	human knee articular cartilage	819:848	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	1	23	theme	proton	121:126	arg1	exchange					137:144	proton chemical exchange	121:144	proton chemical exchange in knee cartilage	121:162	PURPOSE To quantify the characteristics of proton chemical exchange in knee cartilage in vivo by R1ρ dispersion analysis.
25093631	6	24	theme	greater	1239:1245	arg1	contributions					1247:1259	greater contributions	1239:1259	greater contributions from chemical exchange	1239:1282	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	1	25	theme	exchange	137:144	arg1	characteristics					102:116	the characteristics	98:116	the characteristics of proton chemical exchange in knee cartilage	98:162	PURPOSE To quantify the characteristics of proton chemical exchange in knee cartilage in vivo by R1ρ dispersion analysis.
25093631	9	26	theme	quantitative	1640:1651	arg1	evaluation					1653:1662	quantitative evaluation	1640:1662	quantitative evaluation of cartilage disorders	1640:1685	CONCLUSION R1ρ dispersion analysis may provide more specific information for studying cartilage biochemical composition and form the basis for quantitative evaluation of cartilage disorders.
25093631	2	27	theme	cartilage	323:331	arg1	imaging					307:313	T1ρ imaging	303:313	T1ρ imaging of knee cartilage	303:331	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	4	28	from	information	744:754	arg1	composition					766:776	tissue composition	759:776	tissue composition	759:776	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	1	29	theme	knee	149:152	arg1	cartilage					154:162	knee cartilage	149:162	knee cartilage	149:162	PURPOSE To quantify the characteristics of proton chemical exchange in knee cartilage in vivo by R1ρ dispersion analysis.
25093631	9	30	theme	disorders	1677:1685	arg1	evaluation					1653:1662	quantitative evaluation	1640:1662	quantitative evaluation of cartilage disorders	1640:1685	CONCLUSION R1ρ dispersion analysis may provide more specific information for studying cartilage biochemical composition and form the basis for quantitative evaluation of cartilage disorders.
25093631	5	31	from	hydroxyls	937:945	arg1	glycosaminoglycans					958:975	glycosaminoglycans	958:975	glycosaminoglycans	958:975	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	5	32	dep	in	793:794	arg1	vivo					796:799	vivo	796:799	vivo	796:799	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	0	33	theme	Chemical	0:7	arg1	exchange					9:16	Chemical exchange	0:16	Chemical exchange in knee cartilage	0:34	Chemical exchange in knee cartilage assessed by R1ρ (1/T1ρ) dispersion at 3T.
25093631	5	34	theme	articular	830:838	arg1	cartilage					840:848	human knee articular cartilage	819:848	human knee articular cartilage	819:848	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	4	35	theme	dispersion	643:652	arg1	curves					654:659	R1ρ dispersion curves	639:659	R1ρ dispersion curves	639:659	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	2	36	theme	MRI	341:343	arg1	scanner					345:351	a 3T MRI scanner	336:351	a 3T MRI scanner	336:351	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	9	37	dep	CONCLUSION	1497:1506	arg1	analysis					1523:1530	R1ρ dispersion analysis	1508:1530	CONCLUSION R1ρ dispersion analysis	1497:1530	CONCLUSION R1ρ dispersion analysis may provide more specific information for studying cartilage biochemical composition and form the basis for quantitative evaluation of cartilage disorders.
25093631	7	38	theme	1.0-3.0kHz	1382:1391	arg1	range					1373:1377	the range	1369:1377	the range of 1.0-3.0kHz	1369:1391	From the theoretical model, the exchange rates in cartilage were estimated to be in the range of 1.0-3.0kHz, and varied within the tissue.
25093631	5	39	theme	protons	896:902	arg1	rates					887:891	the exchange rates	874:891	the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans)	874:976	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	5	40	dep	estimated	983:991	arg1	based					993:997	based	993:997	were estimated based on a theoretical model	978:1020	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	4	41	with	R1ρ	537:539	arg1	strength					559:566	spin-locking strength	546:566	spin-locking strength (the R1ρ dispersion)	546:587	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	4	41	with	R1ρ	537:539	arg1	dispersion					577:586	the R1ρ dispersion	569:586	the R1ρ dispersion	569:586	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	3	42	theme	spin-lock	421:429	arg1	durations					431:439	5 different spin-lock durations	409:439	5 different spin-lock durations for each of 12 different spin-lock amplitudes	409:485	Quantitative estimates of R1ρ (=1/T1ρ) were made using 5 different spin-lock durations for each of 12 different spin-lock amplitudes over the range 0 to 550Hz.
25093631	6	43	dep	RESULTS	1023:1029	arg1	values					1035:1040	R1ρ values	1031:1040	RESULTS R1ρ values	1023:1040	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	6	44	theme	field	1165:1169	arg1	values					1143:1148	low and high values	1130:1148	low and high values of the locking field, a change much greater than in surrounding tissues	1130:1220	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	9	45	theme	dispersion	1512:1521	arg1	analysis					1523:1530	R1ρ dispersion analysis	1508:1530	CONCLUSION R1ρ dispersion analysis	1497:1530	CONCLUSION R1ρ dispersion analysis may provide more specific information for studying cartilage biochemical composition and form the basis for quantitative evaluation of cartilage disorders.
25093631	5	46	theme	in	793:794	arg1	dispersion					805:814	in vivo R1ρ dispersion	793:814	in vivo R1ρ dispersion of human knee articular cartilage at 3T	793:854	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	7	47	theme	exchange	1317:1324	arg1	rates					1326:1330	the exchange rates	1313:1330	the exchange rates in cartilage	1313:1343	From the theoretical model, the exchange rates in cartilage were estimated to be in the range of 1.0-3.0kHz, and varied within the tissue.
25093631	6	48	theme	marked	1049:1054	arg1	dispersion					1056:1065	marked dispersion	1049:1065	marked dispersion in articular cartilage	1049:1088	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	4	49	theme	spin-locking	546:557	arg1	strength					559:566	spin-locking strength	546:566	spin-locking strength (the R1ρ dispersion)	546:587	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	4	49	theme	spin-locking	546:557	arg1	dispersion					577:586	the R1ρ dispersion	569:586	the R1ρ dispersion	569:586	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	2	50	theme	age	271:273	arg1	range					275:279	age range	271:279	age range 24 to 71 y	271:290	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	7	51	from	rates	1326:1330	arg1	cartilage					1335:1343	cartilage	1335:1343	cartilage	1335:1343	From the theoretical model, the exchange rates in cartilage were estimated to be in the range of 1.0-3.0kHz, and varied within the tissue.
25093631	6	52	theme	surrounding	1202:1212	arg1	tissues					1214:1220	surrounding tissues	1202:1220	surrounding tissues	1202:1220	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	3	53	theme	different	456:464	arg1	amplitudes					476:485	12 different spin-lock amplitudes	453:485	12 different spin-lock amplitudes	453:485	Quantitative estimates of R1ρ (=1/T1ρ) were made using 5 different spin-lock durations for each of 12 different spin-lock amplitudes over the range 0 to 550Hz.
25093631	5	54	from	water	912:916	arg1	glycosaminoglycans					958:975	glycosaminoglycans	958:975	glycosaminoglycans	958:975	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	2	55	theme	T1ρ	303:305	arg1	imaging					307:313	T1ρ imaging	303:313	T1ρ imaging of knee cartilage	303:331	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	1	56	theme	R1ρ	175:177	arg1	analysis					190:197	R1ρ dispersion analysis	175:197	R1ρ dispersion analysis	175:197	PURPOSE To quantify the characteristics of proton chemical exchange in knee cartilage in vivo by R1ρ dispersion analysis.
25093631	4	57	theme	R1ρ	537:539	arg1	variations					523:532	the variations	519:532	the variations of R1ρ with spin-locking strength (the R1ρ dispersion)	519:587	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	5	58	theme	R1ρ	801:803	arg1	dispersion					805:814	in vivo R1ρ dispersion	793:814	in vivo R1ρ dispersion of human knee articular cartilage at 3T	793:854	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	9	59	theme	biochemical	1593:1603	arg1	composition					1605:1615	cartilage biochemical composition	1583:1615	cartilage biochemical composition	1583:1615	CONCLUSION R1ρ dispersion analysis may provide more specific information for studying cartilage biochemical composition and form the basis for quantitative evaluation of cartilage disorders.
25093631	6	60	from	exchange	1275:1282	arg1	contributions					1247:1259	greater contributions	1239:1259	greater contributions from chemical exchange	1239:1282	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	0	61	from	3T	74:75	arg1	dispersion					60:69	R1ρ (1/T1ρ) dispersion	48:69	R1ρ (1/T1ρ) dispersion at 3T	48:75	Chemical exchange in knee cartilage assessed by R1ρ (1/T1ρ) dispersion at 3T.
25093631	4	62	theme	chemical	606:613	arg1	contributions					624:636	chemical exchange contributions	606:636	chemical exchange contributions	606:636	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	6	63	theme	chemical	1266:1273	arg1	exchange					1275:1282	chemical exchange	1266:1282	chemical exchange	1266:1282	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	4	64	theme	R1ρ	573:575	arg1	strength					559:566	spin-locking strength	546:566	spin-locking strength (the R1ρ dispersion)	546:587	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	4	64	theme	R1ρ	573:575	arg1	dispersion					577:586	the R1ρ dispersion	569:586	the R1ρ dispersion	569:586	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	5	65	theme	theoretical	1004:1014	arg1	model					1016:1020	a theoretical model	1002:1020	a theoretical model	1002:1020	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	6	66	with	consistent	1223:1232	arg1	contributions					1247:1259	greater contributions	1239:1259	greater contributions from chemical exchange	1239:1282	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	1	67	theme	chemical	128:135	arg1	exchange					137:144	proton chemical exchange	121:144	proton chemical exchange in knee cartilage	121:162	PURPOSE To quantify the characteristics of proton chemical exchange in knee cartilage in vivo by R1ρ dispersion analysis.
25093631	0	68	from	exchange	9:16	arg1	cartilage					26:34	knee cartilage	21:34	knee cartilage	21:34	Chemical exchange in knee cartilage assessed by R1ρ (1/T1ρ) dispersion at 3T.
25093631	2	69	dep	female	248:253	arg1	range					275:279	age range	271:279	age range 24 to 71 y	271:290	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	1	70	from	exchange	137:144	arg1	cartilage					154:162	knee cartilage	149:162	knee cartilage	149:162	PURPOSE To quantify the characteristics of proton chemical exchange in knee cartilage in vivo by R1ρ dispersion analysis.
25093631	6	71	from	dispersion	1056:1065	arg1	cartilage					1080:1088	articular cartilage	1070:1088	articular cartilage	1070:1088	RESULTS R1ρ values showed marked dispersion in articular cartilage and varied by approximately 50% between low and high values of the locking field, a change much greater than in surrounding tissues, consistent with greater contributions from chemical exchange.
25093631	9	72	theme	cartilage	1667:1675	arg1	disorders					1677:1685	cartilage disorders	1667:1685	cartilage disorders	1667:1685	CONCLUSION R1ρ dispersion analysis may provide more specific information for studying cartilage biochemical composition and form the basis for quantitative evaluation of cartilage disorders.
25093631	2	73	theme	knee	318:321	arg1	cartilage					323:331	knee cartilage	318:331	knee cartilage	318:331	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	1	74	from	characteristics	102:116	arg1	cartilage					154:162	knee cartilage	149:162	knee cartilage	149:162	PURPOSE To quantify the characteristics of proton chemical exchange in knee cartilage in vivo by R1ρ dispersion analysis.
25093631	5	75	theme	knee	825:828	arg1	cartilage					840:848	human knee articular cartilage	819:848	human knee articular cartilage	819:848	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	2	76	dep	range	275:279	arg1	y					290:290	24 to 71 y	281:290	age range 24 to 71 y	271:290	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	0	77	theme	knee	21:24	arg1	cartilage					26:34	knee cartilage	21:34	knee cartilage	21:34	Chemical exchange in knee cartilage assessed by R1ρ (1/T1ρ) dispersion at 3T.
25093631	5	78	theme	cartilage	840:848	arg1	dispersion					805:814	in vivo R1ρ dispersion	793:814	in vivo R1ρ dispersion of human knee articular cartilage at 3T	793:854	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	4	79	theme	R1ρ	639:641	arg1	curves					654:659	R1ρ dispersion curves	639:659	R1ρ dispersion curves	639:659	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	2	80	theme	3T	338:339	arg1	scanner					345:351	a 3T MRI scanner	336:351	a 3T MRI scanner	336:351	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	3	81	theme	Quantitative	354:365	arg1	estimates					367:375	Quantitative estimates	354:375	Quantitative estimates of R1ρ (=1/T1ρ)	354:391	Quantitative estimates of R1ρ (=1/T1ρ) were made using 5 different spin-lock durations for each of 12 different spin-lock amplitudes over the range 0 to 550Hz.
25093631	3	82	theme	R1ρ	380:382	arg1	estimates					367:375	Quantitative estimates	354:375	Quantitative estimates of R1ρ (=1/T1ρ)	354:391	Quantitative estimates of R1ρ (=1/T1ρ) were made using 5 different spin-lock durations for each of 12 different spin-lock amplitudes over the range 0 to 550Hz.
25093631	0	83	theme	R1ρ	48:50	arg1	dispersion					60:69	R1ρ (1/T1ρ) dispersion	48:69	R1ρ (1/T1ρ) dispersion at 3T	48:75	Chemical exchange in knee cartilage assessed by R1ρ (1/T1ρ) dispersion at 3T.
25093631	8	84	theme	single	1444:1449	arg1	knee					1451:1454	a single knee	1442:1454	a single knee	1442:1454	Variations within a single knee appear to be larger with increasing age.
25093631	4	85	theme	exchange	723:730	arg1	characteristics					700:714	quantitative characteristics	687:714	quantitative characteristics of the exchange	687:730	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
25093631	5	86	dep	glycosaminoglycans	958:975	arg1	mainly					948:953	mainly	948:953	mainly	948:953	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	2	87	dep	MATERIALS	200:208	arg1	female					248:253	female	248:253	female	248:253	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	2	87	dep	MATERIALS	200:208	arg1	males					264:268	five males	259:268	five males	259:268	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	2	87	dep	MATERIALS	200:208	arg1	subjects					234:241	Six healthy subjects	222:241	Six healthy subjects	222:241	MATERIALS AND METHODS Six healthy subjects (one female and five males, age range 24 to 71 y) underwent T1ρ imaging of knee cartilage on a 3T MRI scanner.
25093631	5	88	theme	exchange	878:885	arg1	rates					887:891	the exchange rates	874:891	the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans)	874:976	In this work, in vivo R1ρ dispersion of human knee articular cartilage at 3T was analyzed, and the exchange rates of protons between water and macromolecular hydroxyls (mainly in glycosaminoglycans) were estimated based on a theoretical model.
25093631	4	89	theme	quantitative	687:698	arg1	characteristics					700:714	quantitative characteristics	687:714	quantitative characteristics of the exchange	687:730	When the variations of R1ρ with spin-locking strength (the R1ρ dispersion) are dominated by chemical exchange contributions, R1ρ dispersion curves can be analyzed to derive quantitative characteristics of the exchange and provide information on tissue composition.
27112251	0	0	theme	microbial	64:72	arg1	dysbiosis					74:82	gut microbial dysbiosis	60:82	gut microbial dysbiosis in mice	60:90	Acute dextran sulfate sodium (DSS)-induced colitis promotes gut microbial dysbiosis in mice.
27112251	6	1	theme	phylum	840:845	arg1	levels					857:862	phylum and genus levels	840:862	phylum and genus levels	840:862	Alpha- and beta-diversities, and compositional differences at phylum and genus levels were determined, and bacterial functional pathways were predicted.
27112251	9	2	from	pathways	1331:1338	arg1	mucosa					1356:1361	colonic mucosa	1348:1361	colonic mucosa	1348:1361	Also, DSS altered microbial functional pathways in both colonic mucosa and fecal samples.
27112251	9	2	from	pathways	1331:1338	arg1	samples					1373:1379	fecal samples	1367:1379	fecal samples	1367:1379	Also, DSS altered microbial functional pathways in both colonic mucosa and fecal samples.
27112251	1	3	theme	characterized	129:141	arg1	erosion					207:213	the epithelial erosion	192:213	the epithelial erosion	192:213	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	1	3	theme	characterized	129:141	arg1	model					156:160	UNLABELLED The most widely used and characterized experimental model	93:160	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC)	93:187	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	9	4	theme	colonic	1348:1354	arg1	mucosa					1356:1361	colonic mucosa	1348:1361	colonic mucosa	1348:1361	Also, DSS altered microbial functional pathways in both colonic mucosa and fecal samples.
27112251	0	5	theme	gut	60:62	arg1	dysbiosis					74:82	gut microbial dysbiosis	60:82	gut microbial dysbiosis in mice	60:90	Acute dextran sulfate sodium (DSS)-induced colitis promotes gut microbial dysbiosis in mice.
27112251	5	6	used	used	665:668	arg2	samples					652:658	fecal samples	646:658	fecal samples	646:658	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	5	6	used	used	665:668	arg2	mucosa					635:640	Colonic mucosa	627:640	Colonic mucosa	627:640	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	3	7	theme	%	460:460	arg1	DSS					462:464	5% DSS	459:464	5% DSS in drinking water for 5 days	459:493	C57Bl/6 mice received 5% DSS in drinking water for 5 days.
27112251	10	8	theme	taxonomical	1524:1534	arg1	levels					1536:1541	lower taxonomical levels	1518:1541	lower taxonomical levels	1518:1541	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	1	9	theme	DSS	302:304	arg1	administration					284:297	administration	284:297	administration of DSS in drinking water	284:322	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	7	10	theme	species	1027:1033	arg1	richness					1035:1042	bacterial species richness	1017:1042	bacterial species richness	1017:1042	DSS increased disease severity, serum CRP and cytokines IL-1β and IL-6, but decreased bacterial species richness, and shifted bacterial community composition.
27112251	10	11	theme	lower	1518:1522	arg1	levels					1536:1541	lower taxonomical levels	1518:1541	lower taxonomical levels	1518:1541	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	6	12	theme	compositional	811:823	arg1	differences					825:835	Alpha- and beta-diversities, and compositional differences	778:835	differences	825:835	Alpha- and beta-diversities, and compositional differences at phylum and genus levels were determined, and bacterial functional pathways were predicted.
27112251	3	13	from	DSS	462:464	arg1	water					478:482	drinking water	469:482	drinking water	469:482	C57Bl/6 mice received 5% DSS in drinking water for 5 days.
27112251	8	14	theme	colonic	1275:1281	arg1	samples					1283:1289	fecal and colonic samples	1265:1289	fecal and colonic samples	1265:1289	Bacteroides, Turicibacter, Escherichia, Clostridium, Enterobacteriaceae, Clostridiaceae, Bacteroidaceae, Bacteroidales, among other taxa were associated with DSS treatment in fecal and colonic samples.
27112251	9	15	theme	fecal	1367:1371	arg1	samples					1373:1379	fecal samples	1367:1379	fecal samples	1367:1379	Also, DSS altered microbial functional pathways in both colonic mucosa and fecal samples.
27112251	4	16	theme	cytokine	568:575	arg1	levels					577:582	colonic tissue cytokine levels	553:582	colonic tissue cytokine levels	553:582	Inflammation was evaluated clinically and by analysis of colonic tissue cytokine levels and C-reactive protein (CRP) in the serum.
27112251	3	17	theme	drinking	469:476	arg1	water					478:482	drinking water	469:482	drinking water	469:482	C57Bl/6 mice received 5% DSS in drinking water for 5 days.
27112251	5	18	theme	bacterial	710:718	arg1	gene					729:732	bacterial 16S rRNA gene	710:732	bacterial 16S rRNA gene	710:732	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	7	19	theme	community	1067:1075	arg1	composition					1077:1087	bacterial community composition	1057:1087	bacterial community composition	1057:1087	DSS increased disease severity, serum CRP and cytokines IL-1β and IL-6, but decreased bacterial species richness, and shifted bacterial community composition.
27112251	10	20	theme	DSS	1424:1426	arg1	model					1428:1432	DSS model	1424:1432	DSS model	1424:1432	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	10	21	theme	gut	1500:1502	arg1	microbiota					1504:1513	gut microbiota	1500:1513	gut microbiota	1500:1513	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	4	22	theme	protein	599:605	arg1	analysis					541:548	analysis	541:548	analysis of colonic tissue cytokine levels and C-reactive protein (CRP)	541:611	Inflammation was evaluated clinically and by analysis of colonic tissue cytokine levels and C-reactive protein (CRP) in the serum.
27112251	5	23	theme	16S	720:722	arg1	gene					729:732	bacterial 16S rRNA gene	710:732	bacterial 16S rRNA gene	710:732	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	7	24	dep	cytokines	977:985	arg1	IL-1β					987:991	IL-1β	987:991	IL-1β	987:991	DSS increased disease severity, serum CRP and cytokines IL-1β and IL-6, but decreased bacterial species richness, and shifted bacterial community composition.
27112251	7	24	dep	cytokines	977:985	arg1	cytokines					977:985	cytokines	977:985	cytokines IL-1β and IL-6	977:1000	DSS increased disease severity, serum CRP and cytokines IL-1β and IL-6, but decreased bacterial species richness, and shifted bacterial community composition.
27112251	7	24	dep	cytokines	977:985	arg1	IL-6					997:1000	IL-6	997:1000	IL-6	997:1000	DSS increased disease severity, serum CRP and cytokines IL-1β and IL-6, but decreased bacterial species richness, and shifted bacterial community composition.
27112251	1	25	theme	ulcerative	165:174	arg1	UC					185:186	UC	185:186	UC	185:186	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	1	25	theme	ulcerative	165:174	arg1	colitis					176:182	ulcerative colitis	165:182	ulcerative colitis (UC)	165:187	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	5	26	theme	rRNA	724:727	arg1	gene					729:732	bacterial 16S rRNA gene	710:732	bacterial 16S rRNA gene	710:732	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	6	27	theme	functional	895:904	arg1	pathways					906:913	bacterial functional pathways	885:913	bacterial functional pathways	885:913	Alpha- and beta-diversities, and compositional differences at phylum and genus levels were determined, and bacterial functional pathways were predicted.
27112251	1	28	theme	colitis	176:182	arg1	erosion					207:213	the epithelial erosion	192:213	the epithelial erosion	192:213	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	1	28	theme	colitis	176:182	arg1	model					156:160	UNLABELLED The most widely used and characterized experimental model	93:160	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC)	93:187	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	2	29	theme	functional	392:401	arg1	changes					403:409	functional changes	392:409	functional changes in mice treated with DSS	392:434	We investigated fecal and colonic mucosa microbial composition and functional changes in mice treated with DSS.
27112251	6	30	theme	bacterial	885:893	arg1	pathways					906:913	bacterial functional pathways	885:913	bacterial functional pathways	885:913	Alpha- and beta-diversities, and compositional differences at phylum and genus levels were determined, and bacterial functional pathways were predicted.
27112251	5	31	theme	Colonic	627:633	arg1	mucosa					635:640	Colonic mucosa	627:640	Colonic mucosa	627:640	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	8	32	from	treatment	1252:1260	arg1	samples					1283:1289	fecal and colonic samples	1265:1289	fecal and colonic samples	1265:1289	Bacteroides, Turicibacter, Escherichia, Clostridium, Enterobacteriaceae, Clostridiaceae, Bacteroidaceae, Bacteroidales, among other taxa were associated with DSS treatment in fecal and colonic samples.
27112251	8	33	theme	fecal	1265:1269	arg1	samples					1283:1289	fecal and colonic samples	1265:1289	fecal and colonic samples	1265:1289	Bacteroides, Turicibacter, Escherichia, Clostridium, Enterobacteriaceae, Clostridiaceae, Bacteroidaceae, Bacteroidales, among other taxa were associated with DSS treatment in fecal and colonic samples.
27112251	9	34	theme	microbial	1310:1318	arg1	pathways					1331:1338	microbial functional pathways	1310:1338	microbial functional pathways in both colonic mucosa and fecal samples	1310:1379	Also, DSS altered microbial functional pathways in both colonic mucosa and fecal samples.
27112251	4	35	theme	levels	577:582	arg1	analysis					541:548	analysis	541:548	analysis of colonic tissue cytokine levels and C-reactive protein (CRP)	541:611	Inflammation was evaluated clinically and by analysis of colonic tissue cytokine levels and C-reactive protein (CRP) in the serum.
27112251	10	36	from	levels	1536:1541	arg1	dysbiosis					1487:1495	dysbiosis	1487:1495	dysbiosis	1487:1495	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	10	36	from	levels	1536:1541	arg1	diversity					1473:1481	diversity	1473:1481	diversity	1473:1481	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	1	37	theme	-induced	244:251	arg1	erosion					207:213	the epithelial erosion	192:213	the epithelial erosion	192:213	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	1	37	theme	-induced	244:251	arg1	colitis					253:259	dextran sulfate sodium (DSS)-induced colitis	216:259	dextran sulfate sodium (DSS)-induced colitis	216:259	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	1	38	theme	experimental	143:154	arg1	erosion					207:213	the epithelial erosion	192:213	the epithelial erosion	192:213	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	1	38	theme	experimental	143:154	arg1	model					156:160	UNLABELLED The most widely used and characterized experimental model	93:160	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC)	93:187	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	2	39	theme	microbial	366:374	arg1	composition					376:386	microbial composition	366:386	microbial composition	366:386	We investigated fecal and colonic mucosa microbial composition and functional changes in mice treated with DSS.
27112251	5	40	theme	V4	697:698	arg1	region					700:705	the V4 region	693:705	the V4 region of bacterial 16S rRNA gene	693:732	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	5	41	theme	MiSeq	751:755	arg1	sequencing					766:775	MiSeq Illumina sequencing	751:775	MiSeq Illumina sequencing	751:775	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	4	42	theme	C-reactive	588:597	arg1	CRP					608:610	CRP	608:610	CRP	608:610	Inflammation was evaluated clinically and by analysis of colonic tissue cytokine levels and C-reactive protein (CRP) in the serum.
27112251	4	42	theme	C-reactive	588:597	arg1	protein					599:605	C-reactive protein	588:605	C-reactive protein (CRP)	588:611	Inflammation was evaluated clinically and by analysis of colonic tissue cytokine levels and C-reactive protein (CRP) in the serum.
27112251	5	43	theme	fecal	646:650	arg1	samples					652:658	fecal samples	646:658	fecal samples	646:658	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	10	44	dep	CONCLUSIONS	1382:1392	arg1	accompanied					1438:1448	accompanied	1438:1448	was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels	1434:1541	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	5	45	theme	gene	729:732	arg1	region					700:705	the V4 region	693:705	the V4 region of bacterial 16S rRNA gene	693:732	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	5	46	theme	Illumina	757:764	arg1	sequencing					766:775	MiSeq Illumina sequencing	751:775	MiSeq Illumina sequencing	751:775	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	2	47	theme	colonic	351:357	arg1	mucosa					359:364	colonic mucosa	351:364	colonic mucosa	351:364	We investigated fecal and colonic mucosa microbial composition and functional changes in mice treated with DSS.
27112251	0	48	theme	-induced	34:41	arg1	colitis					43:49	Acute dextran sulfate sodium (DSS)-induced colitis	0:49	Acute dextran sulfate sodium (DSS)-induced colitis	0:49	Acute dextran sulfate sodium (DSS)-induced colitis promotes gut microbial dysbiosis in mice.
27112251	10	49	from	development	1398:1408	arg1	model					1428:1432	DSS model	1424:1432	DSS model	1424:1432	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	6	50	from	levels	857:862	arg1	beta-diversities					789:804	beta-diversities	789:804	beta-diversities	789:804	Alpha- and beta-diversities, and compositional differences at phylum and genus levels were determined, and bacterial functional pathways were predicted.
27112251	6	50	from	levels	857:862	arg1	Alpha-					778:783	Alpha-	778:783	Alpha-	778:783	Alpha- and beta-diversities, and compositional differences at phylum and genus levels were determined, and bacterial functional pathways were predicted.
27112251	6	50	from	levels	857:862	arg1	differences					825:835	Alpha- and beta-diversities, and compositional differences	778:835	differences	825:835	Alpha- and beta-diversities, and compositional differences at phylum and genus levels were determined, and bacterial functional pathways were predicted.
27112251	1	51	theme	UNLABELLED	93:102	arg1	erosion					207:213	the epithelial erosion	192:213	the epithelial erosion	192:213	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	1	51	theme	UNLABELLED	93:102	arg1	model					156:160	UNLABELLED The most widely used and characterized experimental model	93:160	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC)	93:187	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	2	52	from	changes	403:409	arg1	mice					414:417	mice	414:417	mice treated with DSS	414:434	We investigated fecal and colonic mucosa microbial composition and functional changes in mice treated with DSS.
27112251	10	53	theme	microbial	1463:1471	arg1	diversity					1473:1481	diversity	1473:1481	diversity	1473:1481	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	7	54	theme	bacterial	1017:1025	arg1	richness					1035:1042	bacterial species richness	1017:1042	bacterial species richness	1017:1042	DSS increased disease severity, serum CRP and cytokines IL-1β and IL-6, but decreased bacterial species richness, and shifted bacterial community composition.
27112251	10	55	theme	microbiota	1504:1513	arg1	dysbiosis					1487:1495	dysbiosis	1487:1495	dysbiosis	1487:1495	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	10	55	theme	microbiota	1504:1513	arg1	diversity					1473:1481	diversity	1473:1481	diversity	1473:1481	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	10	56	theme	colitis	1413:1419	arg1	development					1398:1408	The development	1394:1408	The development of colitis in DSS model	1394:1432	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	6	57	theme	genus	851:855	arg1	levels					857:862	phylum and genus levels	840:862	phylum and genus levels	840:862	Alpha- and beta-diversities, and compositional differences at phylum and genus levels were determined, and bacterial functional pathways were predicted.
27112251	10	58	theme	reduced	1455:1461	arg1	diversity					1473:1481	diversity	1473:1481	diversity	1473:1481	CONCLUSIONS The development of colitis in DSS model was accompanied with reduced microbial diversity and dysbiosis of gut microbiota at lower taxonomical levels.
27112251	7	59	theme	bacterial	1057:1065	arg1	composition					1077:1087	bacterial community composition	1057:1087	bacterial community composition	1057:1087	DSS increased disease severity, serum CRP and cytokines IL-1β and IL-6, but decreased bacterial species richness, and shifted bacterial community composition.
27112251	2	60	from	fecal	341:345	arg1	mice					414:417	mice	414:417	mice treated with DSS	414:434	We investigated fecal and colonic mucosa microbial composition and functional changes in mice treated with DSS.
27112251	8	61	theme	DSS	1248:1250	arg1	treatment					1252:1260	DSS treatment	1248:1260	DSS treatment in fecal and colonic samples	1248:1289	Bacteroides, Turicibacter, Escherichia, Clostridium, Enterobacteriaceae, Clostridiaceae, Bacteroidaceae, Bacteroidales, among other taxa were associated with DSS treatment in fecal and colonic samples.
27112251	8	62	theme	other	1216:1220	arg1	taxa					1222:1225	other taxa	1216:1225	other taxa	1216:1225	Bacteroides, Turicibacter, Escherichia, Clostridium, Enterobacteriaceae, Clostridiaceae, Bacteroidaceae, Bacteroidales, among other taxa were associated with DSS treatment in fecal and colonic samples.
27112251	7	63	theme	serum	963:967	arg1	CRP					969:971	serum CRP	963:971	serum CRP	963:971	DSS increased disease severity, serum CRP and cytokines IL-1β and IL-6, but decreased bacterial species richness, and shifted bacterial community composition.
27112251	7	64	theme	disease	945:951	arg1	severity					953:960	disease severity	945:960	disease severity	945:960	DSS increased disease severity, serum CRP and cytokines IL-1β and IL-6, but decreased bacterial species richness, and shifted bacterial community composition.
27112251	2	65	from	mucosa	359:364	arg1	mice					414:417	mice	414:417	mice treated with DSS	414:434	We investigated fecal and colonic mucosa microbial composition and functional changes in mice treated with DSS.
27112251	3	66	theme	C57Bl/6	437:443	arg1	mice					445:448	C57Bl/6 mice	437:448	C57Bl/6 mice	437:448	C57Bl/6 mice received 5% DSS in drinking water for 5 days.
27112251	1	67	theme	epithelial	196:205	arg1	erosion					207:213	the epithelial erosion	192:213	the epithelial erosion	192:213	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	1	67	theme	epithelial	196:205	arg1	model					156:160	UNLABELLED The most widely used and characterized experimental model	93:160	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC)	93:187	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	1	67	theme	epithelial	196:205	arg1	colitis					253:259	dextran sulfate sodium (DSS)-induced colitis	216:259	dextran sulfate sodium (DSS)-induced colitis	216:259	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	5	68	theme	DNA	674:676	arg1	extraction					678:687	DNA extraction	674:687	DNA extraction	674:687	Colonic mucosa and fecal samples were used for DNA extraction and the V4 region of bacterial 16S rRNA gene was subjected to MiSeq Illumina sequencing.
27112251	0	69	from	dysbiosis	74:82	arg1	mice					87:90	mice	87:90	mice	87:90	Acute dextran sulfate sodium (DSS)-induced colitis promotes gut microbial dysbiosis in mice.
27112251	1	70	theme	used	120:123	arg1	erosion					207:213	the epithelial erosion	192:213	the epithelial erosion	192:213	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	1	70	theme	used	120:123	arg1	model					156:160	UNLABELLED The most widely used and characterized experimental model	93:160	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC)	93:187	UNLABELLED The most widely used and characterized experimental model of ulcerative colitis (UC) is the epithelial erosion, dextran sulfate sodium (DSS)-induced colitis, which is developed by administration of DSS in drinking water.
27112251	4	71	theme	tissue	561:566	arg1	levels					577:582	colonic tissue cytokine levels	553:582	colonic tissue cytokine levels	553:582	Inflammation was evaluated clinically and by analysis of colonic tissue cytokine levels and C-reactive protein (CRP) in the serum.
27112251	2	72	dep	fecal	341:345	arg1	composition					376:386	microbial composition	366:386	microbial composition	366:386	We investigated fecal and colonic mucosa microbial composition and functional changes in mice treated with DSS.
27112251	9	73	theme	functional	1320:1329	arg1	pathways					1331:1338	microbial functional pathways	1310:1338	microbial functional pathways in both colonic mucosa and fecal samples	1310:1379	Also, DSS altered microbial functional pathways in both colonic mucosa and fecal samples.
27112251	4	74	theme	colonic	553:559	arg1	levels					577:582	colonic tissue cytokine levels	553:582	colonic tissue cytokine levels	553:582	Inflammation was evaluated clinically and by analysis of colonic tissue cytokine levels and C-reactive protein (CRP) in the serum.
24959918	0	0	theme	seeded-growth	77:89	arg1	method					91:96	a surfactant-less seeded-growth method	59:96	a surfactant-less seeded-growth method	59:96	Simple control of surface topography of gold nanoshells by a surfactant-less seeded-growth method.
24959918	6	1	theme	cell	1103:1106	arg1	components					1108:1117	intracellular cell components	1089:1117	intracellular cell components than classical smoother nanoshells	1089:1152	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	6	2	theme	nanostars	992:1000	arg1	features					960:967	the features	956:967	the features of gold nanoshells and nanostars	956:1000	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	0	3	theme	surfactant-less	61:75	arg1	method					91:96	a surfactant-less seeded-growth method	59:96	a surfactant-less seeded-growth method	59:96	Simple control of surface topography of gold nanoshells by a surfactant-less seeded-growth method.
24959918	6	4	theme	intracellular	1089:1101	arg1	components					1108:1117	intracellular cell components	1089:1117	intracellular cell components than classical smoother nanoshells	1089:1152	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	4	5	dep	size	645:648	arg1	the					636:638	the	636:638	the	636:638	Chitosan and AA were crucial in determining the BGNS size and structure, acting both as coreductants and structure directing growth agents.
24959918	2	6	theme	acid	296:299	arg1	NPs					316:318	NPs	316:318	NPs	316:318	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	2	6	theme	acid	296:299	arg1	nanoparticles					301:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles	238:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs)	238:319	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	4	7	theme	BGNS	640:643	arg1	size					645:648	BGNS size	640:648	BGNS size	640:648	Chitosan and AA were crucial in determining the BGNS size and structure, acting both as coreductants and structure directing growth agents.
24959918	6	8	theme	nanoshells	977:986	arg1	features					960:967	the features	956:967	the features of gold nanoshells and nanostars	956:1000	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	6	9	theme	gold	972:975	arg1	nanoshells					977:986	gold nanoshells	972:986	gold nanoshells	972:986	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	6	10	theme	enhanced	1041:1048	arg1	probes					1069:1074	surface enhanced Raman spectroscopy probes	1033:1074	surface enhanced Raman spectroscopy probes	1033:1074	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	6	10	theme	enhanced	1041:1048	arg1	performance					1018:1028	a better performance	1009:1028	a better performance	1009:1028	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	2	11	theme	lactic-co-gycolic	277:293	arg1	NPs					316:318	NPs	316:318	NPs	316:318	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	2	11	theme	lactic-co-gycolic	277:293	arg1	nanoparticles					301:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles	238:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs)	238:319	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	2	12	theme	electrostatic	367:379	arg1	agent					397:401	an electrostatic self-assembling agent	364:401	an electrostatic self-assembling agent	364:401	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	2	12	theme	electrostatic	367:379	arg1	chitosan					352:359	chitosan	352:359	chitosan	352:359	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	6	13	theme	smoother	1134:1141	arg1	nanoshells					1143:1152	classical smoother nanoshells	1124:1152	classical smoother nanoshells	1124:1152	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	3	14	theme	growth	533:538	arg1	solution					540:547	HAuCl4/K2CO3 growth solution	520:547	HAuCl4/K2CO3 growth solution	520:547	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	3	14	theme	growth	533:538	arg1	acid					559:562	ascorbic acid	550:562	ascorbic acid (AA)	550:567	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	3	14	theme	growth	533:538	arg1	precursor					581:589	NP-seed precursor	573:589	NP-seed precursor	573:589	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	5	15	theme	biomedical	853:862	arg1	applications					864:875	biomedical applications	853:875	biomedical applications	853:875	Preliminary cytotoxicity experiments point to the biocompatibility of the obtained BGNS, allowing their potential use in biomedical applications.
24959918	4	16	theme	growth	717:722	arg1	agents					724:729	growth agents	717:729	growth agents	717:729	Chitosan and AA were crucial in determining the BGNS size and structure, acting both as coreductants and structure directing growth agents.
24959918	0	17	theme	Simple	0:5	arg1	control					7:13	Simple control	0:13	Simple control of surface topography of gold nanoshells by a surfactant-less seeded-growth method.	0:97	Simple control of surface topography of gold nanoshells by a surfactant-less seeded-growth method.
24959918	1	18	theme	seeded-growth	168:180	arg1	method					198:203	a seeded-growth surfactant-less method	166:203	a seeded-growth surfactant-less method	166:203	We report the synthesis of branched gold nanoshells (BGNS) through a seeded-growth surfactant-less method.
24959918	0	19	theme	surface	18:24	arg1	topography					26:35	surface topography	18:35	surface topography of gold nanoshells	18:54	Simple control of surface topography of gold nanoshells by a surfactant-less seeded-growth method.
24959918	5	20	theme	obtained	806:813	arg1	BGNS					815:818	the obtained BGNS	802:818	the obtained BGNS	802:818	Preliminary cytotoxicity experiments point to the biocompatibility of the obtained BGNS, allowing their potential use in biomedical applications.
24959918	3	21	theme	ascorbic	550:557	arg1	solution					540:547	HAuCl4/K2CO3 growth solution	520:547	HAuCl4/K2CO3 growth solution	520:547	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	3	21	theme	ascorbic	550:557	arg1	acid					559:562	ascorbic acid	550:562	ascorbic acid (AA)	550:567	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	3	21	theme	ascorbic	550:557	arg1	AA					565:566	AA	565:566	AA	565:566	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	1	22	theme	surfactant-less	182:196	arg1	method					198:203	a seeded-growth surfactant-less method	166:203	a seeded-growth surfactant-less method	166:203	We report the synthesis of branched gold nanoshells (BGNS) through a seeded-growth surfactant-less method.
24959918	6	23	theme	classical	1124:1132	arg1	nanoshells					1143:1152	classical smoother nanoshells	1124:1152	classical smoother nanoshells	1124:1152	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	3	24	theme	HAuCl4/K2CO3	520:531	arg1	solution					540:547	HAuCl4/K2CO3 growth solution	520:547	HAuCl4/K2CO3 growth solution	520:547	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	3	24	theme	HAuCl4/K2CO3	520:531	arg1	acid					559:562	ascorbic acid	550:562	ascorbic acid (AA)	550:567	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	3	24	theme	HAuCl4/K2CO3	520:531	arg1	precursor					581:589	NP-seed precursor	573:589	NP-seed precursor	573:589	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	2	25	dep	seeds	329:333	arg1	NP-seed					336:342	NP-seed	336:342	NP-seed	336:342	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	3	26	theme	optical	461:467	arg1	response					469:476	optical response	461:476	optical response	461:476	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	6	27	theme	surface	1033:1039	arg1	probes					1069:1074	surface enhanced Raman spectroscopy probes	1033:1074	surface enhanced Raman spectroscopy probes	1033:1074	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	6	27	theme	surface	1033:1039	arg1	performance					1018:1028	a better performance	1009:1028	a better performance	1009:1028	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	3	28	theme	NP-seed	573:579	arg1	solution					540:547	HAuCl4/K2CO3 growth solution	520:547	HAuCl4/K2CO3 growth solution	520:547	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	3	28	theme	NP-seed	573:579	arg1	precursor					581:589	NP-seed precursor	573:589	NP-seed precursor	573:589	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	6	29	theme	spectroscopy	1056:1067	arg1	probes					1069:1074	surface enhanced Raman spectroscopy probes	1033:1074	surface enhanced Raman spectroscopy probes	1033:1074	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	6	29	theme	spectroscopy	1056:1067	arg1	performance					1018:1028	a better performance	1009:1028	a better performance	1009:1028	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	5	30	theme	potential	836:844	arg1	use					846:848	their potential use	830:848	their potential use in biomedical applications	830:875	Preliminary cytotoxicity experiments point to the biocompatibility of the obtained BGNS, allowing their potential use in biomedical applications.
24959918	0	31	theme	topography	26:35	arg1	control					7:13	Simple control	0:13	Simple control of surface topography of gold nanoshells by a surfactant-less seeded-growth method.	0:97	Simple control of surface topography of gold nanoshells by a surfactant-less seeded-growth method.
24959918	3	32	theme	Branched	404:411	arg1	shells					413:418	Branched shells	404:418	Branched shells with different degrees of anisotropy and optical response	404:476	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	5	33	theme	BGNS	815:818	arg1	biocompatibility					782:797	the biocompatibility	778:797	the biocompatibility of the obtained BGNS	778:818	Preliminary cytotoxicity experiments point to the biocompatibility of the obtained BGNS, allowing their potential use in biomedical applications.
24959918	2	34	theme	self-assembling	381:395	arg1	agent					397:401	an electrostatic self-assembling agent	364:401	an electrostatic self-assembling agent	364:401	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	2	34	theme	self-assembling	381:395	arg1	chitosan					352:359	chitosan	352:359	chitosan	352:359	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	0	35	theme	nanoshells	45:54	arg1	topography					26:35	surface topography	18:35	surface topography of gold nanoshells	18:54	Simple control of surface topography of gold nanoshells by a surfactant-less seeded-growth method.
24959918	3	36	with	shells	413:418	arg1	degrees					435:441	different degrees	425:441	different degrees of anisotropy and optical response	425:476	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	3	37	theme	different	425:433	arg1	degrees					435:441	different degrees	425:441	different degrees of anisotropy and optical response	425:476	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	5	38	from	use	846:848	arg1	applications					864:875	biomedical applications	853:875	biomedical applications	853:875	Preliminary cytotoxicity experiments point to the biocompatibility of the obtained BGNS, allowing their potential use in biomedical applications.
24959918	0	39	theme	gold	40:43	arg1	nanoshells					45:54	gold nanoshells	40:54	gold nanoshells	40:54	Simple control of surface topography of gold nanoshells by a surfactant-less seeded-growth method.
24959918	6	40	with	nanostructures	899:912	arg1	compositions					928:939	"hybrid" compositions	919:939	"hybrid" compositions	919:939	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	6	41	theme	"	926:926	arg1	compositions					928:939	"hybrid" compositions	919:939	"hybrid" compositions	919:939	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	2	42	theme	poly	272:275	arg1	NPs					316:318	NPs	316:318	NPs	316:318	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	2	42	theme	poly	272:275	arg1	nanoparticles					301:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles	238:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs)	238:319	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	6	43	theme	better	1011:1016	arg1	probes					1069:1074	surface enhanced Raman spectroscopy probes	1033:1074	surface enhanced Raman spectroscopy probes	1033:1074	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	6	43	theme	better	1011:1016	arg1	performance					1018:1028	a better performance	1009:1028	a better performance	1009:1028	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	2	44	theme	Au	326:327	arg1	seeds					329:333	Au seeds	326:333	Au seeds (NP-seed)	326:343	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	4	45	dep	coreductants	680:691	arg1	both					672:675	both	672:675	both	672:675	Chitosan and AA were crucial in determining the BGNS size and structure, acting both as coreductants and structure directing growth agents.
24959918	2	46	theme	stabilized	261:270	arg1	NPs					316:318	NPs	316:318	NPs	316:318	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	2	46	theme	stabilized	261:270	arg1	nanoparticles					301:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles	238:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs)	238:319	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	1	47	theme	branched	126:133	arg1	nanoshells					140:149	branched gold nanoshells	126:149	branched gold nanoshells (BGNS)	126:156	We report the synthesis of branched gold nanoshells (BGNS) through a seeded-growth surfactant-less method.
24959918	1	47	theme	branched	126:133	arg1	BGNS					152:155	BGNS	152:155	BGNS	152:155	We report the synthesis of branched gold nanoshells (BGNS) through a seeded-growth surfactant-less method.
24959918	3	48	theme	response	469:476	arg1	degrees					435:441	different degrees	425:441	different degrees of anisotropy and optical response	425:476	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	5	49	theme	Preliminary	732:742	arg1	experiments					757:767	Preliminary cytotoxicity experiments	732:767	Preliminary cytotoxicity experiments	732:767	Preliminary cytotoxicity experiments point to the biocompatibility of the obtained BGNS, allowing their potential use in biomedical applications.
24959918	3	50	theme	anisotropy	446:455	arg1	degrees					435:441	different degrees	425:441	different degrees of anisotropy and optical response	425:476	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	2	51	theme	F127	256:259	arg1	NPs					316:318	NPs	316:318	NPs	316:318	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	2	51	theme	F127	256:259	arg1	nanoparticles					301:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles	238:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs)	238:319	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	6	52	theme	Raman	1050:1054	arg1	probes					1069:1074	surface enhanced Raman spectroscopy probes	1033:1074	surface enhanced Raman spectroscopy probes	1033:1074	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	6	52	theme	Raman	1050:1054	arg1	performance					1018:1028	a better performance	1009:1028	a better performance	1009:1028	In particular, these nanostructures with "hybrid" compositions, which combine the features of gold nanoshells and nanostars showed a better performance as surface enhanced Raman spectroscopy probes in detecting intracellular cell components than classical smoother nanoshells.
24959918	1	53	theme	gold	135:138	arg1	nanoshells					140:149	branched gold nanoshells	126:149	branched gold nanoshells (BGNS)	126:156	We report the synthesis of branched gold nanoshells (BGNS) through a seeded-growth surfactant-less method.
24959918	1	53	theme	gold	135:138	arg1	BGNS					152:155	BGNS	152:155	BGNS	152:155	We report the synthesis of branched gold nanoshells (BGNS) through a seeded-growth surfactant-less method.
24959918	5	54	theme	cytotoxicity	744:755	arg1	experiments					757:767	Preliminary cytotoxicity experiments	732:767	Preliminary cytotoxicity experiments	732:767	Preliminary cytotoxicity experiments point to the biocompatibility of the obtained BGNS, allowing their potential use in biomedical applications.
24959918	3	55	theme	solution	540:547	arg1	ratios					510:515	the ratios	506:515	the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor	506:589	Branched shells with different degrees of anisotropy and optical response were obtained by modulating the ratios of HAuCl4/K2CO3 growth solution, ascorbic acid (AA) and NP-seed precursor.
24959918	2	56	theme	chitosan-Pluronic	238:254	arg1	NPs					316:318	NPs	316:318	NPs	316:318	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	2	56	theme	chitosan-Pluronic	238:254	arg1	nanoparticles					301:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles	238:313	chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs)	238:319	This was achieved by decorating chitosan-Pluronic F127 stabilized poly(lactic-co-gycolic) acid nanoparticles (NPs) with Au seeds (NP-seed), using chitosan as an electrostatic self-assembling agent.
24959918	1	57	theme	nanoshells	140:149	arg1	synthesis					113:121	the synthesis	109:121	the synthesis of branched gold nanoshells (BGNS)	109:156	We report the synthesis of branched gold nanoshells (BGNS) through a seeded-growth surfactant-less method.
28920829	4	0	with	A4α	442:444	arg1	bridge					485:490	an l-Lys-l-Ser-d-Asp interpeptide bridge	451:490	an l-Lys-l-Ser-d-Asp interpeptide bridge	451:490	The peptidoglycan type of strain CPCC 204077T was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge.
28920829	12	1	theme	genus	1734:1738	arg1	species					1717:1723	a novel species	1709:1723	a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp	1709:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	2	2	theme	surface-sterilized	258:275	arg1	root					277:280	the surface-sterilized root	254:280	the surface-sterilized root of a medicinal plant Huperzia serrata	254:318	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	11	3	theme	signature	1529:1537	arg1	nucleotides					1539:1549	the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides	1486:1549	the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides	1486:1549	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	2	4	dep	isolated	240:247	arg1	Thunb					321:325	Thunb	321:325	Thunb	321:325	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	11	5	theme	16S	1520:1522	arg1	nucleotides					1539:1549	the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides	1486:1549	the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides	1486:1549	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	2	6	theme	medicinal	287:295	arg1	serrata					312:318	a medicinal plant Huperzia serrata	285:318	a medicinal plant Huperzia serrata	285:318	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	14	7	theme	110719T=DSM	1857:1867	arg1	29531T					1869:1874	=NBRC 110719T=DSM 29531T	1851:1874	=NBRC 110719T=DSM 29531T	1851:1874	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	14	7	theme	110719T=DSM	1857:1867	arg1	204077T					1842:1848	Strain CPCC 204077T	1830:1848	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T)	1830:1875	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	4	8	theme	204077T	418:424	arg1	type					398:401	The peptidoglycan type	380:401	The peptidoglycan type of strain CPCC 204077T	380:424	The peptidoglycan type of strain CPCC 204077T was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge.
28920829	4	8	theme	204077T	418:424	arg1	A4α					442:444	A4α	442:444	A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge	442:490	The peptidoglycan type of strain CPCC 204077T was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge.
28920829	1	9	attach	isolated	75:82	arg2	Thunb					137:141	Thunb	137:141	Thunb	137:141	nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)
28920829	1	9	attach	isolated	75:82	arg1	root					93:96	the root	89:96	the root of a medicinal plant Huperzia serrata	89:134	nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)
28920829	1	9	attach	isolated	75:82	arg2	Dermacoccaceae					60:73	Dermacoccaceae	60:73	Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)	60:143	nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)
28920829	12	10	with	Dermacoccaceae	1754:1767	arg1	sp					1821:1822	sp	1821:1822	sp	1821:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	12	10	with	Dermacoccaceae	1754:1767	arg1	nov.					1815:1818	the name Allobranchiibius huperziae gen. nov.	1774:1818	the name Allobranchiibius huperziae gen. nov.	1774:1818	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	7	11	theme	lipid	638:642	arg1	profile					644:650	The polar lipid profile	628:650	The polar lipid profile	628:650	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, one unidentified phospholipid and one unidentified glycolipid.
28920829	14	12	theme	CPCC	1837:1840	arg1	strain					1889:1894	the type strain	1880:1894	the type strain of the type species	1880:1914	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	14	12	theme	CPCC	1837:1840	arg1	29531T					1869:1874	=NBRC 110719T=DSM 29531T	1851:1874	=NBRC 110719T=DSM 29531T	1851:1874	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	14	12	theme	CPCC	1837:1840	arg1	204077T					1842:1848	Strain CPCC 204077T	1830:1848	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T)	1830:1875	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	10	13	theme	PC	1243:1244	arg1	IW02T					1246:1250	Calidifontibacter indicus PC IW02T	1217:1250	Calidifontibacter indicus PC IW02T (95.0 %)	1217:1259	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	13	theme	PC	1243:1244	arg1	%					1258:1258	95.0 %	1253:1258	95.0 %	1253:1258	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	5	14	from	compositions	548:559	arg1	hydrolysates					579:590	the whole-cell hydrolysates	564:590	the whole-cell hydrolysates	564:590	Galactose, glucose, rhamnose and ribose were the sugar compositions in the whole-cell hydrolysates.
28920829	12	15	theme	novel	1711:1715	arg1	species					1717:1723	a novel species	1709:1723	a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp	1709:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	2	16	attach	isolated	240:247	arg2	strain					203:208	A Gram-stain-positive, non-spore-forming actinobacterial strain	146:208	A Gram-stain-positive, non-spore-forming actinobacterial strain	146:208	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	2	16	attach	isolated	240:247	arg1	root					277:280	the surface-sterilized root	254:280	the surface-sterilized root of a medicinal plant Huperzia serrata	254:318	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	4	17	theme	strain	406:411	arg1	204077T					418:424	strain CPCC 204077T	406:424	strain CPCC 204077T	406:424	The peptidoglycan type of strain CPCC 204077T was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge.
28920829	11	18	theme	rRNA	1439:1442	arg1	sequence					1444:1451	the 16S rRNA sequence	1431:1451	the 16S rRNA sequence	1431:1451	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	1	19	theme	Huperzia	119:126	arg1	serrata					128:134	a medicinal plant Huperzia serrata	101:134	a medicinal plant Huperzia serrata	101:134	nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)
28920829	4	20	theme	interpeptide	472:483	arg1	bridge					485:490	an l-Lys-l-Ser-d-Asp interpeptide bridge	451:490	an l-Lys-l-Ser-d-Asp interpeptide bridge	451:490	The peptidoglycan type of strain CPCC 204077T was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge.
28920829	10	21	theme	other	1391:1395	arg1	species					1397:1403	all other species	1387:1403	all other species	1387:1403	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	5	22	theme	whole-cell	568:577	arg1	hydrolysates					579:590	the whole-cell hydrolysates	564:590	the whole-cell hydrolysates	564:590	Galactose, glucose, rhamnose and ribose were the sugar compositions in the whole-cell hydrolysates.
28920829	12	23	theme	CPCC	1685:1688	arg1	204077T					1690:1696	strain CPCC 204077T	1678:1696	strain CPCC 204077T	1678:1696	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	4	24	theme	peptidoglycan	384:396	arg1	type					398:401	The peptidoglycan type	380:401	The peptidoglycan type of strain CPCC 204077T	380:424	The peptidoglycan type of strain CPCC 204077T was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge.
28920829	4	24	theme	peptidoglycan	384:396	arg1	A4α					442:444	A4α	442:444	A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge	442:490	The peptidoglycan type of strain CPCC 204077T was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge.
28920829	8	25	theme	major	826:830	arg1	 0					856:857	 0	856:857	 0	856:857	The major fatty acid was iso-C16 : 0.
28920829	8	25	theme	major	826:830	arg1	acid					838:841	The major fatty acid	822:841	The major fatty acid	822:841	The major fatty acid was iso-C16 : 0.
28920829	1	26	theme	medicinal	103:111	arg1	serrata					128:134	a medicinal plant Huperzia serrata	101:134	a medicinal plant Huperzia serrata	101:134	nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)
28920829	6	27	theme	only	610:613	arg1	menaquinone					615:625	the only menaquinone	606:625	the only menaquinone	606:625	MK-8(H4) was the only menaquinone.
28920829	6	27	theme	only	610:613	arg1	MK-8					593:596	MK-8	593:596	MK-8(H4)	593:600	MK-8(H4) was the only menaquinone.
28920829	5	28	theme	sugar	542:546	arg1	ribose					526:531	ribose	526:531	ribose	526:531	Galactose, glucose, rhamnose and ribose were the sugar compositions in the whole-cell hydrolysates.
28920829	5	28	theme	sugar	542:546	arg1	rhamnose					513:520	rhamnose	513:520	rhamnose	513:520	Galactose, glucose, rhamnose and ribose were the sugar compositions in the whole-cell hydrolysates.
28920829	5	28	theme	sugar	542:546	arg1	glucose					504:510	glucose	504:510	glucose	504:510	Galactose, glucose, rhamnose and ribose were the sugar compositions in the whole-cell hydrolysates.
28920829	5	28	theme	sugar	542:546	arg1	compositions					548:559	the sugar compositions	538:559	the sugar compositions in the whole-cell hydrolysates	538:590	Galactose, glucose, rhamnose and ribose were the sugar compositions in the whole-cell hydrolysates.
28920829	5	28	theme	sugar	542:546	arg1	Galactose					493:501	Galactose	493:501	Galactose	493:501	Galactose, glucose, rhamnose and ribose were the sugar compositions in the whole-cell hydrolysates.
28920829	10	29	theme	phylogenetic	907:918	arg1	tree					920:923	The phylogenetic tree	903:923	The phylogenetic tree based on 16S rRNA gene sequences	903:956	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	14	30	theme	species	1908:1914	arg1	strain					1889:1894	the type strain	1880:1894	the type strain of the type species	1880:1914	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	14	30	theme	species	1908:1914	arg1	204077T					1842:1848	Strain CPCC 204077T	1830:1848	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T)	1830:1875	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	0	31	theme	Allobranchiibius	0:15	arg1	nov.					32:35	Allobranchiibius huperziae gen. nov.	0:35	Allobranchiibius huperziae gen. nov.	0:35	Allobranchiibius huperziae gen. nov., sp.
28920829	10	32	theme	gene	1144:1147	arg1	similarities					1158:1169	the highest 16S rRNA gene sequence similarities	1123:1169	the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species	1123:1403	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	33	theme	family	1030:1035	arg1	Dermacoccaceaealongside					1037:1059	the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas	1026:1115	the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas	1026:1115	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	34	theme	gene	943:946	arg1	sequences					948:956	16S rRNA gene sequences	934:956	16S rRNA gene sequences	934:956	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	12	35	from	genus	1734:1738	arg1	Dermacoccaceae					1754:1767	the family Dermacoccaceae	1743:1767	the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp	1743:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	3	36	theme	Sichuan	344:350	arg1	Province					352:359	Sichuan Province	344:359	Sichuan Province	344:359	collected from Sichuan Province, south-west China.
28920829	3	36	theme	Sichuan	344:350	arg1	China					373:377	south-west China	362:377	south-west China	362:377	collected from Sichuan Province, south-west China.
28920829	9	37	theme	genomic	864:870	arg1	G+C content					876:886	The genomic DNA G+C content	860:886	The genomic DNA G+C content	860:886	The genomic DNA G+C content was 71.0 mol%.
28920829	9	37	theme	genomic	864:870	arg1	%					900:900	71.0 mol%	892:900	71.0 mol%	892:900	The genomic DNA G+C content was 71.0 mol%.
28920829	0	38	theme	gen.	27:30	arg1	nov.					32:35	Allobranchiibius huperziae gen. nov.	0:35	Allobranchiibius huperziae gen. nov.	0:35	Allobranchiibius huperziae gen. nov., sp.
28920829	10	39	theme	16S	1135:1137	arg1	similarities					1158:1169	the highest 16S rRNA gene sequence similarities	1123:1169	the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species	1123:1403	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	40	theme	16S	934:936	arg1	sequences					948:956	16S rRNA gene sequences	934:956	16S rRNA gene sequences	934:956	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	41	theme	Calidifontibacter	1217:1233	arg1	IW02T					1246:1250	Calidifontibacter indicus PC IW02T	1217:1250	Calidifontibacter indicus PC IW02T (95.0 %)	1217:1259	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	41	theme	Calidifontibacter	1217:1233	arg1	%					1258:1258	95.0 %	1253:1258	95.0 %	1253:1258	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	42	dep	similarities	1371:1382	arg1	%					1360:1360	%	1360:1360	%	1360:1360	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	43	theme	Branchiibius	1174:1185	arg1	29717T					1200:1205	Branchiibius hedensis Mer 29717T	1174:1205	Branchiibius hedensis Mer 29717T (95.0 %)	1174:1214	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	43	theme	Branchiibius	1174:1185	arg1	%					1213:1213	95.0 %	1208:1213	95.0 %	1208:1213	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	44	theme	strain	970:975	arg1	stood					990:994	that strain CPCC 204077T stood	965:994	that strain CPCC 204077T stood for a distinct lineage	965:1017	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	12	45	theme	huperziae	1800:1808	arg1	sp					1821:1822	sp	1821:1822	sp	1821:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	12	45	theme	huperziae	1800:1808	arg1	nov.					1815:1818	the name Allobranchiibius huperziae gen. nov.	1774:1818	the name Allobranchiibius huperziae gen. nov.	1774:1818	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	12	46	from	species	1717:1723	arg1	Dermacoccaceae					1754:1767	the family Dermacoccaceae	1743:1767	the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp	1743:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	9	47	theme	71.0 mol	892:899	arg1	G+C content					876:886	The genomic DNA G+C content	860:886	The genomic DNA G+C content	860:886	The genomic DNA G+C content was 71.0 mol%.
28920829	9	47	theme	71.0 mol	892:899	arg1	%					900:900	71.0 mol%	892:900	71.0 mol%	892:900	The genomic DNA G+C content was 71.0 mol%.
28920829	11	48	theme	diagnostic	1572:1581	arg1	pattern					1604:1610	a genus-specific diagnostic nucleotide signature pattern	1555:1610	a genus-specific diagnostic nucleotide signature pattern	1555:1610	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	11	49	from	nucleotides	1416:1426	arg1	sequence					1444:1451	the 16S rRNA sequence	1431:1451	the 16S rRNA sequence	1431:1451	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	10	50	theme	sequence	1362:1369	arg1	similarities					1371:1382	less than 94.7 % sequence similarities	1345:1382	less than 94.7 % sequence similarities to all other species	1345:1403	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	2	51	theme	serrata	312:318	arg1	root					277:280	the surface-sterilized root	254:280	the surface-sterilized root of a medicinal plant Huperzia serrata	254:318	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	2	52	dep	Gram-stain-positive	148:166	arg1	non-spore-forming					169:185	non-spore-forming	169:185	non-spore-forming	169:185	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	2	53	theme	Gram-stain-positive	148:166	arg1	strain					203:208	A Gram-stain-positive, non-spore-forming actinobacterial strain	146:208	A Gram-stain-positive, non-spore-forming actinobacterial strain	146:208	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	10	54	dep	%	1360:1360	arg1	94.7 					1355:1359	94.7 	1355:1359	94.7 	1355:1359	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	55	theme	Mer	1196:1198	arg1	29717T					1200:1205	Branchiibius hedensis Mer 29717T	1174:1205	Branchiibius hedensis Mer 29717T (95.0 %)	1174:1214	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	55	theme	Mer	1196:1198	arg1	%					1213:1213	95.0 %	1208:1213	95.0 %	1208:1213	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	56	theme	204077T	982:988	arg1	stood					990:994	that strain CPCC 204077T stood	965:994	that strain CPCC 204077T stood for a distinct lineage	965:1017	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	12	57	theme	name	1778:1781	arg1	sp					1821:1822	sp	1821:1822	sp	1821:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	12	57	theme	name	1778:1781	arg1	nov.					1815:1818	the name Allobranchiibius huperziae gen. nov.	1774:1818	the name Allobranchiibius huperziae gen. nov.	1774:1818	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	11	58	theme	signature	1594:1602	arg1	pattern					1604:1610	a genus-specific diagnostic nucleotide signature pattern	1555:1610	a genus-specific diagnostic nucleotide signature pattern	1555:1610	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	10	59	dep	Branchiibius	1174:1185	arg1	hedensis					1187:1194	hedensis	1187:1194	hedensis	1187:1194	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	12	60	from	Dermacoccaceae	1754:1767	arg1	species					1717:1723	a novel species	1709:1723	a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp	1709:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	11	61	theme	Dermacoccaceaefamily-specific	1490:1518	arg1	nucleotides					1539:1549	the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides	1486:1549	the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides	1486:1549	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	12	62	theme	family	1747:1752	arg1	Dermacoccaceae					1754:1767	the family Dermacoccaceae	1743:1767	the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp	1743:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	14	63	theme	type	1884:1887	arg1	strain					1889:1894	the type strain	1880:1894	the type strain of the type species	1880:1914	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	14	63	theme	type	1884:1887	arg1	204077T					1842:1848	Strain CPCC 204077T	1830:1848	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T)	1830:1875	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	2	64	theme	plant	297:301	arg1	serrata					312:318	a medicinal plant Huperzia serrata	285:318	a medicinal plant Huperzia serrata	285:318	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	12	65	theme	new	1730:1732	arg1	genus					1734:1738	a new genus	1728:1738	a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp	1728:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	11	66	theme	rRNA	1524:1527	arg1	nucleotides					1539:1549	the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides	1486:1549	the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides	1486:1549	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	2	67	theme	CPCC	222:225	arg1	204077T					227:233	CPCC 204077T	222:233	CPCC 204077T	222:233	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	14	68	theme	=NBRC	1851:1855	arg1	29531T					1869:1874	=NBRC 110719T=DSM 29531T	1851:1874	=NBRC 110719T=DSM 29531T	1851:1874	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	14	68	theme	=NBRC	1851:1855	arg1	204077T					1842:1848	Strain CPCC 204077T	1830:1848	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T)	1830:1875	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	10	69	theme	distinct	1002:1009	arg1	lineage					1011:1017	a distinct lineage	1000:1017	a distinct lineage	1000:1017	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	11	70	theme	16S	1435:1437	arg1	rRNA					1439:1442	the 16S rRNA	1431:1442	the 16S rRNA sequence	1431:1451	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	14	71	theme	Strain	1830:1835	arg1	strain					1889:1894	the type strain	1880:1894	the type strain of the type species	1880:1914	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	14	71	theme	Strain	1830:1835	arg1	29531T					1869:1874	=NBRC 110719T=DSM 29531T	1851:1874	=NBRC 110719T=DSM 29531T	1851:1874	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	14	71	theme	Strain	1830:1835	arg1	204077T					1842:1848	Strain CPCC 204077T	1830:1848	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T)	1830:1875	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	8	72	theme	fatty	832:836	arg1	 0					856:857	 0	856:857	 0	856:857	The major fatty acid was iso-C16 : 0.
28920829	8	72	theme	fatty	832:836	arg1	acid					838:841	The major fatty acid	822:841	The major fatty acid	822:841	The major fatty acid was iso-C16 : 0.
28920829	10	73	dep	Dermacoccaceaealongside	1037:1059	arg1	Demetria					1086:1093	Demetria	1086:1093	Demetria	1086:1093	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	73	dep	Dermacoccaceaealongside	1037:1059	arg1	Barrientosiimonas					1099:1115	Barrientosiimonas	1099:1115	Barrientosiimonas	1099:1115	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	4	74	theme	CPCC	413:416	arg1	204077T					418:424	strain CPCC 204077T	406:424	strain CPCC 204077T	406:424	The peptidoglycan type of strain CPCC 204077T was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge.
28920829	10	75	theme	indicus	1235:1241	arg1	IW02T					1246:1250	Calidifontibacter indicus PC IW02T	1217:1250	Calidifontibacter indicus PC IW02T (95.0 %)	1217:1259	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	75	theme	indicus	1235:1241	arg1	%					1258:1258	95.0 %	1253:1258	95.0 %	1253:1258	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	7	76	theme	polar	632:636	arg1	profile					644:650	The polar lipid profile	628:650	The polar lipid profile	628:650	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, one unidentified phospholipid and one unidentified glycolipid.
28920829	1	77	theme	plant	113:117	arg1	serrata					128:134	a medicinal plant Huperzia serrata	101:134	a medicinal plant Huperzia serrata	101:134	nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)
28920829	1	78	theme	serrata	128:134	arg1	root					93:96	the root	89:96	the root of a medicinal plant Huperzia serrata	89:134	nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)
28920829	11	79	contain	contained	1476:1484	arg2	nucleotides					1539:1549	the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides	1486:1549	the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides	1486:1549	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	11	79	contain	contained	1476:1484	arg1	strain					1469:1474	the strain	1465:1474	the strain	1465:1474	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	11	79	contain	contained	1476:1484	arg2	pattern					1604:1610	a genus-specific diagnostic nucleotide signature pattern	1555:1610	a genus-specific diagnostic nucleotide signature pattern	1555:1610	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	1	80	dep	isolated	75:82	arg1	Thunb					137:141	Thunb	137:141	Thunb	137:141	nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)
28920829	1	80	dep	isolated	75:82	arg1	Dermacoccaceae					60:73	Dermacoccaceae	60:73	Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)	60:143	nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)
28920829	12	81	theme	strain	1678:1683	arg1	204077T					1690:1696	strain CPCC 204077T	1678:1696	strain CPCC 204077T	1678:1696	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	12	82	theme	phenotypic	1641:1650	arg1	analyses					1652:1659	the genotypic and phenotypic analyses	1623:1659	the genotypic and phenotypic analyses	1623:1659	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	4	83	theme	l-Lys-l-Ser-d-Asp	454:470	arg1	bridge					485:490	an l-Lys-l-Ser-d-Asp interpeptide bridge	451:490	an l-Lys-l-Ser-d-Asp interpeptide bridge	451:490	The peptidoglycan type of strain CPCC 204077T was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge.
28920829	0	84	theme	huperziae	17:25	arg1	nov.					32:35	Allobranchiibius huperziae gen. nov.	0:35	Allobranchiibius huperziae gen. nov.	0:35	Allobranchiibius huperziae gen. nov., sp.
28920829	1	85	theme	Dermacoccaceae	60:73	arg1	member					50:55	a member	48:55	a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)	48:143	nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)
28920829	1	85	theme	Dermacoccaceae	60:73	arg1	nov.					42:45	nov.	42:45	nov.	42:45	nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)
28920829	3	86	theme	south-west	362:371	arg1	Province					352:359	Sichuan Province	344:359	Sichuan Province	344:359	collected from Sichuan Province, south-west China.
28920829	3	86	theme	south-west	362:371	arg1	China					373:377	south-west China	362:377	south-west China	362:377	collected from Sichuan Province, south-west China.
28920829	11	87	theme	Signature	1406:1414	arg1	nucleotides					1416:1426	Signature nucleotides	1406:1426	Signature nucleotides in the 16S rRNA sequence	1406:1451	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	10	88	theme	rRNA	1139:1142	arg1	similarities					1158:1169	the highest 16S rRNA gene sequence similarities	1123:1169	the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species	1123:1403	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	89	theme	rRNA	938:941	arg1	sequences					948:956	16S rRNA gene sequences	934:956	16S rRNA gene sequences	934:956	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	9	90	theme	DNA	872:874	arg1	G+C content					876:886	The genomic DNA G+C content	860:886	The genomic DNA G+C content	860:886	The genomic DNA G+C content was 71.0 mol%.
28920829	9	90	theme	DNA	872:874	arg1	%					900:900	71.0 mol%	892:900	71.0 mol%	892:900	The genomic DNA G+C content was 71.0 mol%.
28920829	10	91	theme	highest	1127:1133	arg1	similarities					1158:1169	the highest 16S rRNA gene sequence similarities	1123:1169	the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species	1123:1403	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	10	92	theme	CPCC	977:980	arg1	stood					990:994	that strain CPCC 204077T stood	965:994	that strain CPCC 204077T stood for a distinct lineage	965:1017	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	12	93	theme	genotypic	1627:1635	arg1	analyses					1652:1659	the genotypic and phenotypic analyses	1623:1659	the genotypic and phenotypic analyses	1623:1659	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	11	94	theme	genus-specific	1557:1570	arg1	pattern					1604:1610	a genus-specific diagnostic nucleotide signature pattern	1555:1610	a genus-specific diagnostic nucleotide signature pattern	1555:1610	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
28920829	7	95	theme	unidentified	797:808	arg1	glycolipid					810:819	one unidentified glycolipid	793:819	one unidentified glycolipid	793:819	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, one unidentified phospholipid and one unidentified glycolipid.
28920829	0	96	dep	sp	38:39	arg1	nov.					32:35	Allobranchiibius huperziae gen. nov.	0:35	Allobranchiibius huperziae gen. nov.	0:35	Allobranchiibius huperziae gen. nov., sp.
28920829	12	97	theme	Allobranchiibius	1783:1798	arg1	sp					1821:1822	sp	1821:1822	sp	1821:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	12	97	theme	Allobranchiibius	1783:1798	arg1	nov.					1815:1818	the name Allobranchiibius huperziae gen. nov.	1774:1818	the name Allobranchiibius huperziae gen. nov.	1774:1818	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	2	98	theme	actinobacterial	187:201	arg1	strain					203:208	A Gram-stain-positive, non-spore-forming actinobacterial strain	146:208	A Gram-stain-positive, non-spore-forming actinobacterial strain	146:208	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	10	99	theme	sequence	1149:1156	arg1	similarities					1158:1169	the highest 16S rRNA gene sequence similarities	1123:1169	the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species	1123:1403	The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077T stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717T (95.0 %), Calidifontibacter indicus PC IW02T (95.0 %), Barrientosiimonas humi 39T (94.9 %) and Demetria terragena HKI 0089T (94.7 %), and less than 94.7 % sequence similarities to all other species.
28920829	14	100	theme	type	1903:1906	arg1	species					1908:1914	the type species	1899:1914	the type species	1899:1914	Strain CPCC 204077T (=NBRC 110719T=DSM 29531T) is the type strain of the type species.
28920829	12	101	theme	gen.	1810:1813	arg1	sp					1821:1822	sp	1821:1822	sp	1821:1822	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	12	101	theme	gen.	1810:1813	arg1	nov.					1815:1818	the name Allobranchiibius huperziae gen. nov.	1774:1818	the name Allobranchiibius huperziae gen. nov.	1774:1818	Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077T represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp.
28920829	2	102	theme	Huperzia	303:310	arg1	serrata					312:318	a medicinal plant Huperzia serrata	285:318	a medicinal plant Huperzia serrata	285:318	A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.)
28920829	7	103	theme	unidentified	763:774	arg1	phospholipid					776:787	one unidentified phospholipid	759:787	one unidentified phospholipid	759:787	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, one unidentified phospholipid and one unidentified glycolipid.
28920829	11	104	theme	nucleotide	1583:1592	arg1	pattern					1604:1610	a genus-specific diagnostic nucleotide signature pattern	1555:1610	a genus-specific diagnostic nucleotide signature pattern	1555:1610	Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern.
25439942	1	0	theme	nanoparticles	249:261	arg1	synthesis					229:237	in situ synthesis	221:237	in situ synthesis of silver nanoparticles (AgNPs)	221:269	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	4	1	theme	antibacterial	698:710	arg1	activities					712:721	the antibacterial activities	694:721	the antibacterial activities	694:721	The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods.
25439942	4	2	theme	flask	794:798	arg1	methods					800:806	dynamic shake flask methods	780:806	dynamic shake flask methods	780:806	The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods.
25439942	2	3	theme	reaction	283:290	arg1	conditions					292:301	reaction conditions	283:301	reaction conditions	283:301	Effects of reaction conditions of the BC/PAM composite were investigated on its microstructure, mechanical properties and thermal stabilities.
25439942	1	4	theme	bacterial	112:120	arg1	template					208:215	the template	204:215	the template for in situ synthesis of silver nanoparticles (AgNPs)	204:269	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	1	4	theme	bacterial	112:120	arg1	composite					171:179	a bacterial cellulose/polyacrylamide (BC/PAM) double network composite	110:179	a bacterial cellulose/polyacrylamide (BC/PAM) double network composite	110:179	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	3	5	used	utilized	458:465	arg2	BC					450:451	Both the BC/PAM composite and pure BC	415:451	Both the BC/PAM composite and pure BC	415:451	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	4	6	theme	inhibition	765:774	arg1	zone					757:760	the zone	753:760	the zone of inhibition	753:774	The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods.
25439942	4	6	theme	inhibition	765:774	arg1	methods					800:806	dynamic shake flask methods	780:806	dynamic shake flask methods	780:806	The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods.
25439942	3	7	theme	corresponding	482:494	arg1	BC-AgNPs					554:561	BC-AgNPs	554:561	BC-AgNPs	554:561	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	3	7	theme	corresponding	482:494	arg1	BC/PAM-AgNPs					537:548	BC/PAM-AgNPs	537:548	BC/PAM-AgNPs	537:548	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	3	7	theme	corresponding	482:494	arg1	nanocomposites					515:528	the corresponding silver impregnated nanocomposites	478:528	the corresponding silver impregnated nanocomposites	478:528	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	1	8	theme	cellulose/polyacrylamide	122:145	arg1	template					208:215	the template	204:215	the template for in situ synthesis of silver nanoparticles (AgNPs)	204:269	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	1	8	theme	cellulose/polyacrylamide	122:145	arg1	composite					171:179	a bacterial cellulose/polyacrylamide (BC/PAM) double network composite	110:179	a bacterial cellulose/polyacrylamide (BC/PAM) double network composite	110:179	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	7	9	theme	close	1105:1109	arg1	effect					1125:1130	a close antibacterial effect	1103:1130	a close antibacterial effect	1103:1130	For the antibacterial test, two nanocomposites exhibited a close antibacterial effect, with a high log reduction above 3 and killing ratio above 99.9%, respectively.
25439942	4	10	theme	shake	788:792	arg1	methods					800:806	dynamic shake flask methods	780:806	dynamic shake flask methods	780:806	The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods.
25439942	5	11	theme	denser	860:865	arg1	microstructure					867:880	a denser microstructure	858:880	a denser microstructure	858:880	It was shown that the BC/PAM composite displayed a denser microstructure and higher thermal stabilities than pure BC.
25439942	6	12	theme	mass	1001:1004	arg1	content					1006:1012	lower mass content	995:1012	lower mass content of AgNPs	995:1021	The BC/PAM-AgNPs nanocomposite exhibited a bigger particle size and lower mass content of AgNPs than the BC-AgNPs one.
25439942	5	13	theme	pure	918:921	arg1	BC					923:924	pure BC	918:924	pure BC	918:924	It was shown that the BC/PAM composite displayed a denser microstructure and higher thermal stabilities than pure BC.
25439942	3	14	dep	Both	415:418	arg1	composite					431:439	the BC/PAM composite	420:439	the BC/PAM composite	420:439	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	4	15	theme	dynamic	780:786	arg1	methods					800:806	dynamic shake flask methods	780:806	dynamic shake flask methods	780:806	The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods.
25439942	2	16	theme	thermal	394:400	arg1	stabilities					402:412	thermal stabilities	394:412	thermal stabilities	394:412	Effects of reaction conditions of the BC/PAM composite were investigated on its microstructure, mechanical properties and thermal stabilities.
25439942	7	17	theme	killing	1171:1177	arg1	ratio					1179:1183	killing ratio	1171:1183	killing ratio	1171:1183	For the antibacterial test, two nanocomposites exhibited a close antibacterial effect, with a high log reduction above 3 and killing ratio above 99.9%, respectively.
25439942	6	18	theme	particle	977:984	arg1	size					986:989	a bigger particle size	968:989	a bigger particle size	968:989	The BC/PAM-AgNPs nanocomposite exhibited a bigger particle size and lower mass content of AgNPs than the BC-AgNPs one.
25439942	2	19	theme	BC/PAM	310:315	arg1	composite					317:325	the BC/PAM composite	306:325	the BC/PAM composite	306:325	Effects of reaction conditions of the BC/PAM composite were investigated on its microstructure, mechanical properties and thermal stabilities.
25439942	6	20	theme	lower	995:999	arg1	content					1006:1012	lower mass content	995:1012	lower mass content of AgNPs	995:1021	The BC/PAM-AgNPs nanocomposite exhibited a bigger particle size and lower mass content of AgNPs than the BC-AgNPs one.
25439942	4	21	theme	nanocomposites	730:743	arg1	activities					712:721	the antibacterial activities	694:721	the antibacterial activities	694:721	The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods.
25439942	4	21	theme	nanocomposites	730:743	arg1	formation					680:688	AgNPs formation	674:688	AgNPs formation	674:688	The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods.
25439942	3	22	theme	friendly	584:591	arg1	method					593:598	an environmental friendly method	567:598	an environmental friendly method	567:598	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	6	23	theme	AgNPs	1017:1021	arg1	size					986:989	a bigger particle size	968:989	a bigger particle size	968:989	The BC/PAM-AgNPs nanocomposite exhibited a bigger particle size and lower mass content of AgNPs than the BC-AgNPs one.
25439942	6	23	theme	AgNPs	1017:1021	arg1	content					1006:1012	lower mass content	995:1012	lower mass content of AgNPs	995:1021	The BC/PAM-AgNPs nanocomposite exhibited a bigger particle size and lower mass content of AgNPs than the BC-AgNPs one.
25439942	4	24	theme	AgNPs	674:678	arg1	formation					680:688	AgNPs formation	674:688	AgNPs formation	674:688	The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods.
25439942	7	25	theme	antibacterial	1111:1123	arg1	effect					1125:1130	a close antibacterial effect	1103:1130	a close antibacterial effect	1103:1130	For the antibacterial test, two nanocomposites exhibited a close antibacterial effect, with a high log reduction above 3 and killing ratio above 99.9%, respectively.
25439942	4	26	theme	templates	639:647	arg1	influences					621:630	The influences	617:630	The influences of the templates	617:647	The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods.
25439942	2	27	theme	conditions	292:301	arg1	Effects					272:278	Effects	272:278	Effects of reaction conditions of the BC/PAM composite	272:325	Effects of reaction conditions of the BC/PAM composite were investigated on its microstructure, mechanical properties and thermal stabilities.
25439942	1	28	theme	double	156:161	arg1	template					208:215	the template	204:215	the template for in situ synthesis of silver nanoparticles (AgNPs)	204:269	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	1	28	theme	double	156:161	arg1	composite					171:179	a bacterial cellulose/polyacrylamide (BC/PAM) double network composite	110:179	a bacterial cellulose/polyacrylamide (BC/PAM) double network composite	110:179	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	3	29	dep	nanocomposites	515:528	arg1	i.e.					531:534	the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs	478:561	i.e.	531:534	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	3	30	theme	UV	601:602	arg1	irradiation					604:614	UV irradiation	601:614	UV irradiation	601:614	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	5	31	theme	higher	886:891	arg1	stabilities					901:911	higher thermal stabilities	886:911	higher thermal stabilities	886:911	It was shown that the BC/PAM composite displayed a denser microstructure and higher thermal stabilities than pure BC.
25439942	3	32	theme	BC/PAM	424:429	arg1	composite					431:439	the BC/PAM composite	420:439	the BC/PAM composite	420:439	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	6	33	theme	bigger	970:975	arg1	size					986:989	a bigger particle size	968:989	a bigger particle size	968:989	The BC/PAM-AgNPs nanocomposite exhibited a bigger particle size and lower mass content of AgNPs than the BC-AgNPs one.
25439942	0	34	theme	BC/PAM-AgNPs	36:47	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of BC/PAM-AgNPs nanocomposites for antibacterial applications.
25439942	0	34	theme	BC/PAM-AgNPs	36:47	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of BC/PAM-AgNPs nanocomposites for antibacterial applications.
25439942	1	35	theme	in	221:222	arg1	synthesis					229:237	in situ synthesis	221:237	in situ synthesis of silver nanoparticles (AgNPs)	221:269	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	0	36	theme	antibacterial	68:80	arg1	applications					82:93	antibacterial applications	68:93	antibacterial applications	68:93	Preparation and characterization of BC/PAM-AgNPs nanocomposites for antibacterial applications.
25439942	5	37	theme	thermal	893:899	arg1	stabilities					901:911	higher thermal stabilities	886:911	higher thermal stabilities	886:911	It was shown that the BC/PAM composite displayed a denser microstructure and higher thermal stabilities than pure BC.
25439942	3	38	theme	silver	496:501	arg1	BC-AgNPs					554:561	BC-AgNPs	554:561	BC-AgNPs	554:561	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	3	38	theme	silver	496:501	arg1	BC/PAM-AgNPs					537:548	BC/PAM-AgNPs	537:548	BC/PAM-AgNPs	537:548	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	3	38	theme	silver	496:501	arg1	nanocomposites					515:528	the corresponding silver impregnated nanocomposites	478:528	the corresponding silver impregnated nanocomposites	478:528	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	7	39	theme	log	1145:1147	arg1	reduction					1149:1157	a high log reduction	1138:1157	a high log reduction above 3	1138:1165	For the antibacterial test, two nanocomposites exhibited a close antibacterial effect, with a high log reduction above 3 and killing ratio above 99.9%, respectively.
25439942	7	40	theme	antibacterial	1054:1066	arg1	test					1068:1071	the antibacterial test	1050:1071	the antibacterial test	1050:1071	For the antibacterial test, two nanocomposites exhibited a close antibacterial effect, with a high log reduction above 3 and killing ratio above 99.9%, respectively.
25439942	3	41	dep	BC	450:451	arg1	pure					445:448	pure	445:448	pure	445:448	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	3	41	dep	BC	450:451	arg1	Both					415:418	Both	415:418	Both	415:418	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	3	42	theme	impregnated	503:513	arg1	BC-AgNPs					554:561	BC-AgNPs	554:561	BC-AgNPs	554:561	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	3	42	theme	impregnated	503:513	arg1	BC/PAM-AgNPs					537:548	BC/PAM-AgNPs	537:548	BC/PAM-AgNPs	537:548	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	3	42	theme	impregnated	503:513	arg1	nanocomposites					515:528	the corresponding silver impregnated nanocomposites	478:528	the corresponding silver impregnated nanocomposites	478:528	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	3	43	theme	environmental	570:582	arg1	method					593:598	an environmental friendly method	567:598	an environmental friendly method	567:598	Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation.
25439942	4	44	dep	formation	680:688	arg1	the					670:672	the	670:672	the	670:672	The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods.
25439942	1	45	theme	BC/PAM	148:153	arg1	template					208:215	the template	204:215	the template for in situ synthesis of silver nanoparticles (AgNPs)	204:269	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	1	45	theme	BC/PAM	148:153	arg1	composite					171:179	a bacterial cellulose/polyacrylamide (BC/PAM) double network composite	110:179	a bacterial cellulose/polyacrylamide (BC/PAM) double network composite	110:179	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	1	46	dep	in	221:222	arg1	situ					224:227	situ	224:227	situ	224:227	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	2	47	theme	composite	317:325	arg1	Effects					272:278	Effects	272:278	Effects of reaction conditions of the BC/PAM composite	272:325	Effects of reaction conditions of the BC/PAM composite were investigated on its microstructure, mechanical properties and thermal stabilities.
25439942	6	48	theme	BC-AgNPs	1032:1039	arg1	one					1041:1043	the BC-AgNPs one	1028:1043	the BC-AgNPs one	1028:1043	The BC/PAM-AgNPs nanocomposite exhibited a bigger particle size and lower mass content of AgNPs than the BC-AgNPs one.
25439942	5	49	theme	BC/PAM	831:836	arg1	composite					838:846	the BC/PAM composite	827:846	the BC/PAM composite	827:846	It was shown that the BC/PAM composite displayed a denser microstructure and higher thermal stabilities than pure BC.
25439942	2	50	theme	mechanical	368:377	arg1	properties					379:388	mechanical properties	368:388	mechanical properties	368:388	Effects of reaction conditions of the BC/PAM composite were investigated on its microstructure, mechanical properties and thermal stabilities.
25439942	7	51	theme	high	1140:1143	arg1	reduction					1149:1157	a high log reduction	1138:1157	a high log reduction above 3	1138:1165	For the antibacterial test, two nanocomposites exhibited a close antibacterial effect, with a high log reduction above 3 and killing ratio above 99.9%, respectively.
25439942	6	52	theme	BC/PAM-AgNPs	931:942	arg1	nanocomposite					944:956	The BC/PAM-AgNPs nanocomposite	927:956	The BC/PAM-AgNPs nanocomposite	927:956	The BC/PAM-AgNPs nanocomposite exhibited a bigger particle size and lower mass content of AgNPs than the BC-AgNPs one.
25439942	1	53	theme	network	163:169	arg1	template					208:215	the template	204:215	the template for in situ synthesis of silver nanoparticles (AgNPs)	204:269	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	1	53	theme	network	163:169	arg1	composite					171:179	a bacterial cellulose/polyacrylamide (BC/PAM) double network composite	110:179	a bacterial cellulose/polyacrylamide (BC/PAM) double network composite	110:179	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	1	54	theme	silver	242:247	arg1	AgNPs					264:268	AgNPs	264:268	AgNPs	264:268	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
25439942	1	54	theme	silver	242:247	arg1	nanoparticles					249:261	silver nanoparticles	242:261	silver nanoparticles (AgNPs)	242:269	In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs).
27349890	7	0	theme	high	1093:1096	arg1	level					1098:1102	high level	1093:1102	high level (7.5wt%)	1093:1111	At high level (7.5wt%), tensile strength, Young's modulus and solubility of the films decreased and elongation at break and moisture content increased due to agglomeration of the fillers.
27349890	7	0	theme	high	1093:1096	arg1	%					1110:1110	7.5wt%	1105:1110	7.5wt%	1105:1110	At high level (7.5wt%), tensile strength, Young's modulus and solubility of the films decreased and elongation at break and moisture content increased due to agglomeration of the fillers.
27349890	8	1	theme	ONC	1354:1356	arg1	incorporation					1358:1370	ONC incorporation	1354:1370	ONC incorporation	1354:1370	Nevertheless, film transparency and water vapor permeability decreased with ONC incorporation.
27349890	1	2	theme	composite	142:150	arg1	films					152:156	Whey protein isolate (WPI)-based composite films	109:156	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis	109:252	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	1	3	theme	acid	229:232	arg1	hydrolysis					243:252	acid sulfuric hydrolysis	229:252	acid sulfuric hydrolysis	229:252	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	4	4	from	husk	791:794	arg1	removal					741:747	the progressive removal	725:747	the progressive removal of non-cellulosic components from the oat husk	725:794	FTIR spectroscopy indicated the progressive removal of non-cellulosic components from the oat husk.
27349890	1	5	with	films	152:156	arg1	proportions					171:181	varying proportions	163:181	varying proportions of oat husk nanocellulose (ONC)	163:213	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	5	6	theme	mean	819:822	arg1	width					824:828	the mean width	815:828	the mean width of ONC	815:835	SEM images showed the mean width of ONC was about 76nm and XRD analysis revealed the crystallinity increased after acid hydrolysis.
27349890	1	7	theme	sulfuric	234:241	arg1	hydrolysis					243:252	acid sulfuric hydrolysis	229:252	acid sulfuric hydrolysis	229:252	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	3	8	theme	WPI	603:605	arg1	WPI					603:605	WPI	603:605	WPI	603:605	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite were then evaluated.
27349890	3	8	theme	WPI	603:605	arg1	%					598:598	0, 2.5, 5 and 7.5wt%	579:598	0, 2.5, 5 and 7.5wt% of WPI	579:605	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite were then evaluated.
27349890	6	9	dep	%	962:962	arg1	5wt					959:961	5wt	959:961	5wt	959:961	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	5	10	dep	showed	808:813	arg1	76nm					847:850	76nm	847:850	76nm	847:850	SEM images showed the mean width of ONC was about 76nm and XRD analysis revealed the crystallinity increased after acid hydrolysis.
27349890	0	11	theme	oat	99:101	arg1	husk					103:106	oat husk	99:106	oat husk	99:106	Properties of whey protein isolate nanocomposite films reinforced with nanocellulose isolated from oat husk.
27349890	6	12	theme	tensile	987:993	arg1	strength					995:1002	tensile strength	987:1002	tensile strength	987:1002	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	6	13	theme	Young	1005:1009	arg1	modulus					1013:1019	Young's modulus	1005:1019	Young's modulus	1005:1019	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	4	14	theme	components	767:776	arg1	removal					741:747	the progressive removal	725:747	the progressive removal of non-cellulosic components from the oat husk	725:794	FTIR spectroscopy indicated the progressive removal of non-cellulosic components from the oat husk.
27349890	6	15	dep	5wt	959:961	arg1	to					956:957	to	956:957	to	956:957	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	2	16	theme	electron	483:490	arg1	microscopy					492:501	Scanning electron microscopy	474:501	Scanning electron microscopy (SEM)	474:507	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	2	16	theme	electron	483:490	arg1	SEM					504:506	SEM	504:506	SEM	504:506	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	2	17	theme	FTIR	454:457	arg1	spectroscopy					460:471	(FTIR) spectroscopy	453:471	(FTIR) spectroscopy	453:471	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	6	18	theme	highest	979:985	arg1	strength					995:1002	tensile strength	987:1002	tensile strength	987:1002	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	1	19	theme	varying	163:169	arg1	proportions					171:181	varying proportions	163:181	varying proportions of oat husk nanocellulose (ONC)	163:213	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	2	20	dep	Fourier	426:432	arg1	transform					434:442	transform	434:442	transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively	434:549	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	7	21	theme	tensile	1114:1120	arg1	strength					1122:1129	tensile strength	1114:1129	tensile strength	1114:1129	At high level (7.5wt%), tensile strength, Young's modulus and solubility of the films decreased and elongation at break and moisture content increased due to agglomeration of the fillers.
27349890	6	22	theme	lowest	1041:1046	arg1	elongation					1048:1057	the lowest elongation	1037:1057	the lowest elongation at break and moisture content	1037:1087	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	6	23	from	break	1062:1066	arg1	strength					995:1002	tensile strength	987:1002	tensile strength	987:1002	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	6	23	from	break	1062:1066	arg1	solubility					1022:1031	solubility	1022:1031	solubility	1022:1031	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	6	23	from	break	1062:1066	arg1	modulus					1013:1019	Young's modulus	1005:1019	Young's modulus	1005:1019	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	6	23	from	break	1062:1066	arg1	elongation					1048:1057	the lowest elongation	1037:1057	the lowest elongation at break and moisture content	1037:1087	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	6	24	dep	ONC	964:966	arg1	%					962:962	%	962:962	%	962:962	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	2	25	theme	cellulose	356:364	arg1	step					334:337	each step	329:337	each step of the isolating cellulose	329:364	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	0	26	theme	protein	19:25	arg1	films					49:53	whey protein isolate nanocomposite films	14:53	whey protein isolate nanocomposite films	14:53	Properties of whey protein isolate nanocomposite films reinforced with nanocellulose isolated from oat husk.
27349890	6	27	dep	strength	995:1002	arg1	the					975:977	the	975:977	the	975:977	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	4	28	theme	oat	787:789	arg1	husk					791:794	the oat husk	783:794	the oat husk	783:794	FTIR spectroscopy indicated the progressive removal of non-cellulosic components from the oat husk.
27349890	7	29	from	content	1223:1229	arg1	elongation					1190:1199	elongation	1190:1199	elongation at break and moisture content	1190:1229	At high level (7.5wt%), tensile strength, Young's modulus and solubility of the films decreased and elongation at break and moisture content increased due to agglomeration of the fillers.
27349890	1	30	theme	oat	186:188	arg1	ONC					210:212	ONC	210:212	ONC	210:212	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	1	30	theme	oat	186:188	arg1	nanocellulose					195:207	oat husk nanocellulose	186:207	oat husk nanocellulose (ONC)	186:213	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	0	31	theme	whey	14:17	arg1	films					49:53	whey protein isolate nanocomposite films	14:53	whey protein isolate nanocomposite films	14:53	Properties of whey protein isolate nanocomposite films reinforced with nanocellulose isolated from oat husk.
27349890	3	32	theme	barrier	636:642	arg1	properties					644:653	barrier properties	636:653	barrier properties	636:653	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite were then evaluated.
27349890	2	33	theme	X-ray	513:517	arg1	XRD					532:534	XRD	532:534	XRD	532:534	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	2	33	theme	X-ray	513:517	arg1	diffraction					519:529	X-ray diffraction	513:529	X-ray diffraction (XRD)	513:535	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	5	34	theme	XRD	856:858	arg1	analysis					860:867	XRD analysis	856:867	XRD analysis	856:867	SEM images showed the mean width of ONC was about 76nm and XRD analysis revealed the crystallinity increased after acid hydrolysis.
27349890	1	35	theme	husk	190:193	arg1	ONC					210:212	ONC	210:212	ONC	210:212	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	1	35	theme	husk	190:193	arg1	nanocellulose					195:207	oat husk nanocellulose	186:207	oat husk nanocellulose (ONC)	186:213	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	0	36	theme	nanocomposite	35:47	arg1	films					49:53	whey protein isolate nanocomposite films	14:53	whey protein isolate nanocomposite films	14:53	Properties of whey protein isolate nanocomposite films reinforced with nanocellulose isolated from oat husk.
27349890	1	37	theme	solution	276:283	arg1	method					293:298	a solution casting method	274:298	a solution casting method	274:298	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	3	38	dep	content	570:576	arg1	WPI					603:605	WPI	603:605	WPI	603:605	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite were then evaluated.
27349890	3	38	dep	content	570:576	arg1	%					598:598	0, 2.5, 5 and 7.5wt%	579:598	0, 2.5, 5 and 7.5wt% of WPI	579:605	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite were then evaluated.
27349890	4	39	theme	progressive	729:739	arg1	removal					741:747	the progressive removal	725:747	the progressive removal of non-cellulosic components from the oat husk	725:794	FTIR spectroscopy indicated the progressive removal of non-cellulosic components from the oat husk.
27349890	3	40	from	effect	556:561	arg1	mechanical					621:630	mechanical	621:630	mechanical	621:630	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite were then evaluated.
27349890	3	40	from	effect	556:561	arg1	properties					644:653	barrier properties	636:653	barrier properties	636:653	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite were then evaluated.
27349890	3	40	from	effect	556:561	arg1	physical					611:618	physical	611:618	physical	611:618	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite were then evaluated.
27349890	1	41	theme	nanocellulose	195:207	arg1	proportions					171:181	varying proportions	163:181	varying proportions of oat husk nanocellulose (ONC)	163:213	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	0	42	theme	isolate	27:33	arg1	films					49:53	whey protein isolate nanocomposite films	14:53	whey protein isolate nanocomposite films	14:53	Properties of whey protein isolate nanocomposite films reinforced with nanocellulose isolated from oat husk.
27349890	1	43	theme	casting	285:291	arg1	method					293:298	a solution casting method	274:298	a solution casting method	274:298	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	8	44	theme	water	1314:1318	arg1	permeability					1326:1337	water vapor permeability	1314:1337	water vapor permeability	1314:1337	Nevertheless, film transparency and water vapor permeability decreased with ONC incorporation.
27349890	7	45	theme	moisture	1214:1221	arg1	content					1223:1229	moisture content	1214:1229	moisture content	1214:1229	At high level (7.5wt%), tensile strength, Young's modulus and solubility of the films decreased and elongation at break and moisture content increased due to agglomeration of the fillers.
27349890	7	46	theme	Young	1132:1136	arg1	modulus					1140:1146	Young's modulus	1132:1146	Young's modulus	1132:1146	At high level (7.5wt%), tensile strength, Young's modulus and solubility of the films decreased and elongation at break and moisture content increased due to agglomeration of the fillers.
27349890	0	47	theme	films	49:53	arg1	Properties					0:9	Properties	0:9	Properties of whey protein isolate nanocomposite films	0:53	Properties of whey protein isolate nanocomposite films reinforced with nanocellulose isolated from oat husk.
27349890	3	48	theme	nanocomposite	662:674	arg1	physical					611:618	physical	611:618	physical	611:618	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite were then evaluated.
27349890	7	49	theme	films	1170:1174	arg1	solubility					1152:1161	solubility	1152:1161	solubility	1152:1161	At high level (7.5wt%), tensile strength, Young's modulus and solubility of the films decreased and elongation at break and moisture content increased due to agglomeration of the fillers.
27349890	7	49	theme	films	1170:1174	arg1	modulus					1140:1146	Young's modulus	1132:1146	Young's modulus	1132:1146	At high level (7.5wt%), tensile strength, Young's modulus and solubility of the films decreased and elongation at break and moisture content increased due to agglomeration of the fillers.
27349890	7	49	theme	films	1170:1174	arg1	strength					1122:1129	tensile strength	1114:1129	tensile strength	1114:1129	At high level (7.5wt%), tensile strength, Young's modulus and solubility of the films decreased and elongation at break and moisture content increased due to agglomeration of the fillers.
27349890	4	50	theme	non-cellulosic	752:765	arg1	components					767:776	non-cellulosic components	752:776	non-cellulosic components	752:776	FTIR spectroscopy indicated the progressive removal of non-cellulosic components from the oat husk.
27349890	0	51	attach	isolated	85:92	arg1	husk					103:106	oat husk	99:106	oat husk	99:106	Properties of whey protein isolate nanocomposite films reinforced with nanocellulose isolated from oat husk.
27349890	0	51	attach	isolated	85:92	arg2	nanocellulose					71:83	nanocellulose	71:83	nanocellulose isolated from oat husk	71:106	Properties of whey protein isolate nanocomposite films reinforced with nanocellulose isolated from oat husk.
27349890	8	52	theme	film	1292:1295	arg1	transparency					1297:1308	film transparency	1292:1308	film transparency	1292:1308	Nevertheless, film transparency and water vapor permeability decreased with ONC incorporation.
27349890	6	53	theme	moisture	1072:1079	arg1	content					1081:1087	moisture content	1072:1087	moisture content	1072:1087	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	8	54	theme	vapor	1320:1324	arg1	permeability					1326:1337	water vapor permeability	1314:1337	water vapor permeability	1314:1337	Nevertheless, film transparency and water vapor permeability decreased with ONC incorporation.
27349890	4	55	theme	FTIR	697:700	arg1	spectroscopy					702:713	FTIR spectroscopy	697:713	FTIR spectroscopy	697:713	FTIR spectroscopy indicated the progressive removal of non-cellulosic components from the oat husk.
27349890	3	56	theme	content	570:576	arg1	effect					556:561	The effect	552:561	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite	552:674	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite were then evaluated.
27349890	7	57	theme	fillers	1269:1275	arg1	agglomeration					1248:1260	agglomeration	1248:1260	agglomeration of the fillers	1248:1275	At high level (7.5wt%), tensile strength, Young's modulus and solubility of the films decreased and elongation at break and moisture content increased due to agglomeration of the fillers.
27349890	2	58	dep	transform	434:442	arg1	infrared					444:451	infrared	444:451	infrared	444:451	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	5	59	theme	SEM	797:799	arg1	images					801:806	SEM images	797:806	SEM images	797:806	SEM images showed the mean width of ONC was about 76nm and XRD analysis revealed the crystallinity increased after acid hydrolysis.
27349890	2	60	theme	obtained	305:312	arg1	material					314:321	The obtained material	301:321	The obtained material	301:321	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	5	61	theme	ONC	833:835	arg1	width					824:828	the mean width	815:828	the mean width of ONC	815:835	SEM images showed the mean width of ONC was about 76nm and XRD analysis revealed the crystallinity increased after acid hydrolysis.
27349890	2	62	theme	Scanning	474:481	arg1	microscopy					492:501	Scanning electron microscopy	474:501	Scanning electron microscopy (SEM)	474:507	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	2	62	theme	Scanning	474:481	arg1	SEM					504:506	SEM	504:506	SEM	504:506	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	5	63	theme	acid	912:915	arg1	hydrolysis					917:926	acid hydrolysis	912:926	acid hydrolysis	912:926	SEM images showed the mean width of ONC was about 76nm and XRD analysis revealed the crystallinity increased after acid hydrolysis.
27349890	2	64	theme	ONC	406:408	arg1	morphological					367:379	morphological	367:379	morphological	367:379	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	2	65	theme	isolating	346:354	arg1	cellulose					356:364	the isolating cellulose	342:364	the isolating cellulose	342:364	The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively.
27349890	7	66	from	break	1204:1208	arg1	elongation					1190:1199	elongation	1190:1199	elongation at break and moisture content	1190:1229	At high level (7.5wt%), tensile strength, Young's modulus and solubility of the films decreased and elongation at break and moisture content increased due to agglomeration of the fillers.
27349890	3	67	theme	ONC	566:568	arg1	content					570:576	ONC content	566:576	ONC content (0, 2.5, 5 and 7.5wt% of WPI)	566:606	The effect of ONC content (0, 2.5, 5 and 7.5wt% of WPI) on physical, mechanical and barrier properties of the nanocomposite were then evaluated.
27349890	1	68	theme	-based	135:140	arg1	films					152:156	Whey protein isolate (WPI)-based composite films	109:156	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis	109:252	Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method.
27349890	6	69	from	content	1081:1087	arg1	strength					995:1002	tensile strength	987:1002	tensile strength	987:1002	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	6	69	from	content	1081:1087	arg1	solubility					1022:1031	solubility	1022:1031	solubility	1022:1031	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	6	69	from	content	1081:1087	arg1	modulus					1013:1019	Young's modulus	1005:1019	Young's modulus	1005:1019	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27349890	6	69	from	content	1081:1087	arg1	elongation					1048:1057	the lowest elongation	1037:1057	the lowest elongation at break and moisture content	1037:1087	The films prepared with up to 5wt% ONC showed the highest tensile strength, Young's modulus, solubility and the lowest elongation at break and moisture content.
27283689	7	0	theme	extruded	1089:1096	arg1	snacks					1098:1103	extruded snacks	1089:1103	extruded snacks	1089:1103	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	3	1	theme	axial	536:540	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	1	theme	axial	536:540	arg1	expansion					542:550	axial expansion	536:550	axial expansion	536:550	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	2	2	theme	pinto	334:338	arg1	flour					345:349	Brown rice: pinto bean flour	322:349	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour)	322:384	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	2	3	dep	extruded	391:398	arg1	%					465:465	20.1%	461:465	20.1%	461:465	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	2	3	dep	extruded	391:398	arg1	%					433:433	17.2%	429:433	17.2%	429:433	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	2	3	dep	extruded	391:398	arg1	%					440:440	18.1%	436:440	18.1%	436:440	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	2	3	dep	extruded	391:398	arg1	%					447:447	18.3%	443:447	18.3%	443:447	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	2	3	dep	extruded	391:398	arg1	%					454:454	19.5%	450:454	19.5%	450:454	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	3	4	theme	absorption	640:649	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	4	theme	absorption	640:649	arg1	index					651:655	water absorption index	634:655	water absorption index	634:655	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	1	5	dep	in	252:253	arg1	vitro					255:259	vitro	255:259	vitro	255:259	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	5	6	theme	bean	898:901	arg1	substitution					903:914	bean substitution	898:914	bean substitution	898:914	Rapidly digestible starch decreased and resistant starch increased as bean substitution and processing moisture increased.
27283689	7	7	theme	physical	1127:1134	arg1	attributes					1136:1145	physical attributes	1127:1145	physical attributes (hardness, density, and expansion)	1127:1180	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	7	7	theme	physical	1127:1134	arg1	density					1158:1164	density	1158:1164	density	1158:1164	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	7	7	theme	physical	1127:1134	arg1	expansion					1171:1179	expansion	1171:1179	expansion	1171:1179	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	7	7	theme	physical	1127:1134	arg1	hardness					1148:1155	hardness	1148:1155	hardness	1148:1155	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	1	8	theme	physical	228:235	arg1	properties					237:246	physical properties	228:246	physical properties	228:246	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	0	9	theme	starch	88:93	arg1	digestibility					71:83	in vitro digestibility	62:83	in vitro digestibility	62:83	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	9	theme	starch	88:93	arg1	properties					47:56	physical properties	38:56	physical properties	38:56	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	10	from	Effects	0:6	arg1	digestibility					71:83	in vitro digestibility	62:83	in vitro digestibility	62:83	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	10	from	Effects	0:6	arg1	properties					47:56	physical properties	38:56	physical properties	38:56	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	3	11	theme	[bulk	489:493	arg1	density					495:501	[bulk density	489:501	[bulk density	489:501	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	11	theme	[bulk	489:493	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	7	12	dep	in	1209:1210	arg1	vitro					1212:1216	vitro	1212:1216	vitro	1212:1216	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	7	13	theme	bean	1073:1076	arg1	flour					1078:1082	bean flour	1073:1082	bean flour	1073:1082	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	0	14	from	properties	47:56	arg1	flours					155:160	pinto bean composite flours	134:160	pinto bean composite flours	134:160	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	14	from	properties	47:56	arg1	rice					125:128	extruded brown rice	110:128	extruded brown rice	110:128	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	3	15	dep	in	662:663	arg1	vitro					665:669	vitro	665:669	vitro	665:669	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	6	16	theme	In	951:952	arg1	digestibility					968:980	In vitro protein digestibility	951:980	In vitro protein digestibility	951:980	In vitro protein digestibility increased with increasing bean flour or with decreasing processing moisture.
27283689	3	17	theme	water	634:638	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	17	theme	water	634:638	arg1	index					651:655	water absorption index	634:655	water absorption index	634:655	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	0	18	theme	protein	99:105	arg1	digestibility					71:83	in vitro digestibility	62:83	in vitro digestibility	62:83	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	18	theme	protein	99:105	arg1	properties					47:56	physical properties	38:56	physical properties	38:56	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	2	19	dep	flour	345:349	arg1	%					353:353	0%	352:353	0%	352:353	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	2	19	dep	flour	345:349	arg1	%					358:358	15%	356:358	15%	356:358	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	2	19	dep	flour	345:349	arg1	%					363:363	30%	361:363	30%	361:363	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	2	19	dep	flour	345:349	arg1	%					372:372	45%	370:372	45%	370:372	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	7	20	theme	in	1209:1210	arg1	decrease					1226:1233	decrease	1226:1233	decrease	1226:1233	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	7	20	theme	in	1209:1210	arg1	starch					1218:1223	in vitro starch (decrease) and protein (increase) digestibilities	1209:1273	starch	1218:1223	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	2	21	theme	moisture	408:415	arg1	conditions					417:426	5 moisture conditions	406:426	5 moisture conditions	406:426	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	3	22	dep	properties	478:487	arg1	index					623:627	water solubility index	606:627	water solubility index	606:627	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	22	dep	properties	478:487	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	22	dep	properties	478:487	arg1	hardness					589:596	hardness	589:596	hardness	589:596	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	22	dep	properties	478:487	arg1	expansion					561:569	overall expansion	553:569	overall expansion	553:569	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	22	dep	properties	478:487	arg1	density					495:501	[bulk density	489:501	[bulk density	489:501	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	22	dep	properties	478:487	arg1	color					599:603	color	599:603	color	599:603	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	22	dep	properties	478:487	arg1	expansion					525:533	radial expansion	518:533	radial expansion	518:533	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	22	dep	properties	478:487	arg1	index					651:655	water absorption index	634:655	water absorption index	634:655	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	22	dep	properties	478:487	arg1	volume					581:586	specific volume	572:586	specific volume	572:586	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	22	dep	properties	478:487	arg1	density					509:515	unit density	504:515	unit density	504:515	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	22	dep	properties	478:487	arg1	expansion					542:550	axial expansion	536:550	axial expansion	536:550	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	23	theme	specific	572:579	arg1	volume					581:586	specific volume	572:586	specific volume	572:586	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	23	theme	specific	572:579	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	24	theme	radial	518:523	arg1	expansion					525:533	radial expansion	518:533	radial expansion	518:533	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	24	theme	radial	518:523	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	1	25	theme	in	252:253	arg1	digestibility					261:273	in vitro digestibility	252:273	in vitro digestibility	252:273	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	1	26	dep	properties	237:246	arg1	the					224:226	the	224:226	the	224:226	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	0	27	theme	moisture	22:29	arg1	Effects					0:6	Effects	0:6	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.	0:161	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	28	theme	extruded	110:117	arg1	rice					125:128	extruded brown rice	110:128	extruded brown rice	110:128	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	29	dep	in	62:63	arg1	vitro					65:69	vitro	65:69	vitro	65:69	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	6	30	dep	In	951:952	arg1	vitro					954:958	vitro	954:958	vitro	954:958	In vitro protein digestibility increased with increasing bean flour or with decreasing processing moisture.
27283689	0	31	theme	processing	11:20	arg1	moisture					22:29	processing moisture	11:29	processing moisture	11:29	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	4	32	theme	bean	734:737	arg1	flour					739:743	bean flour	734:743	bean flour	734:743	Increasing bean flour and processing moisture increased density and hardness while decreasing expansion.
27283689	0	33	from	digestibility	71:83	arg1	flours					155:160	pinto bean composite flours	134:160	pinto bean composite flours	134:160	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	33	from	digestibility	71:83	arg1	rice					125:128	extruded brown rice	110:128	extruded brown rice	110:128	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	6	34	theme	bean	1008:1011	arg1	flour					1013:1017	bean flour	1008:1017	bean flour	1008:1017	In vitro protein digestibility increased with increasing bean flour or with decreasing processing moisture.
27283689	1	35	theme	pinto	180:184	arg1	flour					191:195	pinto bean flour	180:195	pinto bean flour	180:195	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	0	36	from	flours	155:160	arg1	digestibility					71:83	in vitro digestibility	62:83	in vitro digestibility	62:83	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	36	from	flours	155:160	arg1	properties					47:56	physical properties	38:56	physical properties	38:56	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	4	37	theme	processing	749:758	arg1	moisture					760:767	processing moisture	749:767	processing moisture	749:767	Increasing bean flour and processing moisture increased density and hardness while decreasing expansion.
27283689	5	38	theme	processing	920:929	arg1	moisture					931:938	processing moisture	920:938	processing moisture	920:938	Rapidly digestible starch decreased and resistant starch increased as bean substitution and processing moisture increased.
27283689	0	39	dep	properties	47:56	arg1	the					34:36	the	34:36	the	34:36	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	1	40	theme	bean	186:189	arg1	flour					191:195	pinto bean flour	180:195	pinto bean flour	180:195	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	1	41	theme	rice-bean	278:286	arg1	extrudates					288:297	rice-bean extrudates	278:297	rice-bean extrudates	278:297	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	0	42	theme	brown	119:123	arg1	rice					125:128	extruded brown rice	110:128	extruded brown rice	110:128	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	3	43	theme	Physical	469:476	arg1	index					623:627	water solubility index	606:627	water solubility index	606:627	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	43	theme	Physical	469:476	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	43	theme	Physical	469:476	arg1	hardness					589:596	hardness	589:596	hardness	589:596	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	43	theme	Physical	469:476	arg1	expansion					561:569	overall expansion	553:569	overall expansion	553:569	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	43	theme	Physical	469:476	arg1	density					495:501	[bulk density	489:501	[bulk density	489:501	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	43	theme	Physical	469:476	arg1	color					599:603	color	599:603	color	599:603	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	43	theme	Physical	469:476	arg1	expansion					525:533	radial expansion	518:533	radial expansion	518:533	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	43	theme	Physical	469:476	arg1	index					651:655	water absorption index	634:655	water absorption index	634:655	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	43	theme	Physical	469:476	arg1	volume					581:586	specific volume	572:586	specific volume	572:586	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	43	theme	Physical	469:476	arg1	density					509:515	unit density	504:515	unit density	504:515	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	43	theme	Physical	469:476	arg1	expansion					542:550	axial expansion	536:550	axial expansion	536:550	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	44	theme	water	606:610	arg1	index					623:627	water solubility index	606:627	water solubility index	606:627	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	44	theme	water	606:610	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	1	45	theme	flour	191:195	arg1	influence					167:175	The influence	163:175	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates	163:297	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	2	46	theme	bean	374:377	arg1	flour					379:383	bean flour	374:383	bean flour	374:383	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	1	47	theme	extrudates	288:297	arg1	digestibility					261:273	in vitro digestibility	252:273	in vitro digestibility	252:273	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	1	47	theme	extrudates	288:297	arg1	properties					237:246	physical properties	228:246	physical properties	228:246	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	0	48	theme	pinto	134:138	arg1	flours					155:160	pinto bean composite flours	134:160	pinto bean composite flours	134:160	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	5	49	theme	resistant	868:876	arg1	starch					878:883	resistant starch	868:883	resistant starch	868:883	Rapidly digestible starch decreased and resistant starch increased as bean substitution and processing moisture increased.
27283689	2	50	theme	rice	328:331	arg1	flour					345:349	Brown rice: pinto bean flour	322:349	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour)	322:384	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	0	51	theme	physical	38:45	arg1	properties					47:56	physical properties	38:56	physical properties	38:56	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	52	from	protein	99:105	arg1	flours					155:160	pinto bean composite flours	134:160	pinto bean composite flours	134:160	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	52	from	protein	99:105	arg1	rice					125:128	extruded brown rice	110:128	extruded brown rice	110:128	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	7	53	theme	protein	1240:1246	arg1	digestibilities					1259:1273	in vitro starch (decrease) and protein (increase) digestibilities	1209:1273	digestibilities	1259:1273	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	7	53	theme	protein	1240:1246	arg1	increase					1249:1256	increase	1249:1256	increase	1249:1256	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	2	54	theme	Brown	322:326	arg1	flour					345:349	Brown rice: pinto bean flour	322:349	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour)	322:384	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	3	55	theme	in	662:663	arg1	starch					671:676	in vitro starch and protein digestibilities	662:704	starch	671:676	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	0	56	theme	composite	145:153	arg1	flours					155:160	pinto bean composite flours	134:160	pinto bean composite flours	134:160	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	3	57	theme	overall	553:559	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	57	theme	overall	553:559	arg1	expansion					561:569	overall expansion	553:569	overall expansion	553:569	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	6	58	dep	increased	982:990	arg1	decreasing					1027:1036	decreasing	1027:1036	decreasing processing moisture	1027:1056	In vitro protein digestibility increased with increasing bean flour or with decreasing processing moisture.
27283689	6	58	dep	increased	982:990	arg1	increasing					997:1006	increasing	997:1006	increasing bean flour	997:1017	In vitro protein digestibility increased with increasing bean flour or with decreasing processing moisture.
27283689	7	59	dep	attributes	1136:1145	arg1	attributes					1136:1145	physical attributes	1127:1145	physical attributes (hardness, density, and expansion)	1127:1180	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	7	59	dep	attributes	1136:1145	arg1	density					1158:1164	density	1158:1164	density	1158:1164	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	7	59	dep	attributes	1136:1145	arg1	expansion					1171:1179	expansion	1171:1179	expansion	1171:1179	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	7	59	dep	attributes	1136:1145	arg1	hardness					1148:1155	hardness	1148:1155	hardness	1148:1155	Incorporating bean flour into extruded snacks can negatively affect physical attributes (hardness, density, and expansion) while positively affecting in vitro starch (decrease) and protein (increase) digestibilities.
27283689	0	60	from	rice	125:128	arg1	digestibility					71:83	in vitro digestibility	62:83	in vitro digestibility	62:83	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	60	from	rice	125:128	arg1	properties					47:56	physical properties	38:56	physical properties	38:56	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	61	theme	bean	140:143	arg1	flours					155:160	pinto bean composite flours	134:160	pinto bean composite flours	134:160	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	62	from	starch	88:93	arg1	flours					155:160	pinto bean composite flours	134:160	pinto bean composite flours	134:160	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	0	62	from	starch	88:93	arg1	rice					125:128	extruded brown rice	110:128	extruded brown rice	110:128	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	1	63	theme	processing	201:210	arg1	moisture					212:219	processing moisture	201:219	processing moisture	201:219	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	0	64	theme	in	62:63	arg1	digestibility					71:83	in vitro digestibility	62:83	in vitro digestibility	62:83	Effects of processing moisture on the physical properties and in vitro digestibility of starch and protein in extruded brown rice and pinto bean composite flours.
27283689	3	65	theme	unit	504:507	arg1	density					509:515	unit density	504:515	unit density	504:515	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	65	theme	unit	504:507	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	5	66	theme	digestible	836:845	arg1	starch					847:852	Rapidly digestible starch	828:852	Rapidly digestible starch	828:852	Rapidly digestible starch decreased and resistant starch increased as bean substitution and processing moisture increased.
27283689	1	67	from	influence	167:175	arg1	digestibility					261:273	in vitro digestibility	252:273	in vitro digestibility	252:273	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	1	67	from	influence	167:175	arg1	properties					237:246	physical properties	228:246	physical properties	228:246	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	6	68	theme	protein	960:966	arg1	digestibility					968:980	In vitro protein digestibility	951:980	In vitro protein digestibility	951:980	In vitro protein digestibility increased with increasing bean flour or with decreasing processing moisture.
27283689	6	69	theme	processing	1038:1047	arg1	moisture					1049:1056	processing moisture	1038:1056	processing moisture	1038:1056	In vitro protein digestibility increased with increasing bean flour or with decreasing processing moisture.
27283689	1	70	theme	moisture	212:219	arg1	influence					167:175	The influence	163:175	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates	163:297	The influence of pinto bean flour and processing moisture on the physical properties and in vitro digestibility of rice-bean extrudates has been investigated.
27283689	2	71	dep	%	353:353	arg1	flour					379:383	bean flour	374:383	bean flour	374:383	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	2	72	theme	bean	340:343	arg1	flour					345:349	Brown rice: pinto bean flour	322:349	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour)	322:384	Brown rice: pinto bean flour (0%, 15%, 30%, and 45% bean flour) were extruded under 5 moisture conditions (17.2%, 18.1%, 18.3%, 19.5%, and 20.1%).
27283689	3	73	theme	protein	682:688	arg1	digestibilities					690:704	in vitro starch and protein digestibilities	662:704	digestibilities	690:704	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	74	theme	solubility	612:621	arg1	index					623:627	water solubility index	606:627	water solubility index	606:627	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27283689	3	74	theme	solubility	612:621	arg1	properties					478:487	Physical properties	469:487	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index]	469:656	Physical properties [bulk density, unit density, radial expansion, axial expansion, overall expansion, specific volume, hardness, color, water solubility index, and water absorption index] and in vitro starch and protein digestibilities were determined.
27285857	7	0	theme	early	1195:1199	arg1	erosion					1201:1207	the early erosion	1191:1207	the early erosion	1191:1207	During the early erosion, a small amount of debris peeled off and became suspended in the fluid.
27285857	4	1	theme	surface	632:638	arg1	properties					640:649	surface properties	632:649	surface properties	632:649	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	9	2	theme	cell	1566:1569	arg1	adhesion					1571:1578	cell adhesion	1566:1578	cell adhesion	1566:1578	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	8	3	theme	doped	1354:1358	arg1	Ag					1360:1361	the doped Ag	1350:1361	the doped Ag	1350:1361	After that erosion, the composite film became relatively stable, and the doped Ag was slowly released into the fluid.
27285857	9	4	with	comparison	1402:1411	arg1	Ag					1431:1432	the released Ag	1418:1432	the released Ag (either in the peeled debris or dissolved in the fluid)	1418:1488	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	6	5	theme	CS	1140:1141	arg1	matrix					1143:1148	the CS matrix	1136:1148	the CS matrix with a polycrystalline structure	1136:1181	Using statistical analysis, the following conclusions can be made: the doped AgNPs dispersed in the CS matrix with a polycrystalline structure.
27285857	5	6	theme	human	949:953	arg1	HUVEC					989:993	HUVEC	989:993	HUVEC	989:993	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	5	6	theme	human	949:953	arg1	cells					982:986	human umbilical vein endothelial cells	949:986	human umbilical vein endothelial cells (HUVEC)	949:994	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	0	7	theme	higher	93:98	arg1	rate					111:114	a higher inhibition rate	91:114	a higher inhibition rate to cell proliferation	91:136	Cytotoxicity of AgNPs/CS composite films: AgNPs immobilized in chitosan matrix contributes a higher inhibition rate to cell proliferation.
27285857	10	8	theme	cell	1696:1699	arg1	rate					1684:1687	the survival rate	1671:1687	the survival rate of the cell	1671:1699	Film thickness and AgNP content show a synergistic effect on the survival rate of the cell, with the AgNPs content being the key factor.
27285857	7	9	theme	debris	1228:1233	arg1	amount					1218:1223	a small amount	1210:1223	a small amount of debris	1210:1233	During the early erosion, a small amount of debris peeled off and became suspended in the fluid.
27285857	7	9	theme	debris	1228:1233	arg1	debris					1228:1233	debris	1228:1233	debris	1228:1233	During the early erosion, a small amount of debris peeled off and became suspended in the fluid.
27285857	4	10	theme	behavior	701:708	arg1	stabilities					655:665	stabilities	655:665	stabilities	655:665	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	4	10	theme	behavior	701:708	arg1	properties					640:649	surface properties	632:649	surface properties	632:649	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	2	11	theme	interest	494:501	arg1	mechanism					478:486	the dominant mechanism is	465:489	the dominant mechanism is of interest	465:501	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	5	12	theme	cells	982:986	arg1	morphology					856:865	The morphology	852:865	The morphology of adhered cells	852:882	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	5	12	theme	cells	982:986	arg1	rate					901:904	the survival rate	888:904	the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC)	888:994	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	4	13	theme	Ag-releasing	688:699	arg1	behavior					701:708	the Ag-releasing behavior	684:708	the Ag-releasing behavior	684:708	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	2	14	theme	slow-release	271:282	arg1	system					284:289	this slow-release system	266:289	this slow-release system	266:289	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	9	15	theme	subsequent	1584:1593	arg1	proliferation					1595:1607	subsequent proliferation	1584:1607	subsequent proliferation	1584:1607	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	4	16	theme	scanning	760:767	arg1	SEM					790:792	SEM	790:792	SEM	790:792	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	4	16	theme	scanning	760:767	arg1	microscopy					778:787	scanning electron microscopy	760:787	scanning electron microscopy (SEM)	760:793	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	4	17	theme	films	674:678	arg1	stabilities					655:665	stabilities	655:665	stabilities	655:665	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	4	17	theme	films	674:678	arg1	properties					640:649	surface properties	632:649	surface properties	632:649	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	0	18	theme	inhibition	100:109	arg1	rate					111:114	a higher inhibition rate	91:114	a higher inhibition rate to cell proliferation	91:136	Cytotoxicity of AgNPs/CS composite films: AgNPs immobilized in chitosan matrix contributes a higher inhibition rate to cell proliferation.
27285857	6	19	theme	statistical	1046:1056	arg1	analysis					1058:1065	statistical analysis	1046:1065	statistical analysis	1046:1065	Using statistical analysis, the following conclusions can be made: the doped AgNPs dispersed in the CS matrix with a polycrystalline structure.
27285857	9	20	dep	either	1435:1440	arg1	debris					1456:1461	the peeled debris	1445:1461	the peeled debris	1445:1461	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	2	21	theme	surrounding	417:427	arg1	fluid					429:433	the surrounding fluid	413:433	the surrounding fluid	413:433	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	10	22	theme	AgNPs	1711:1715	arg1	content					1717:1723	the AgNPs content	1707:1723	the AgNPs content	1707:1723	Film thickness and AgNP content show a synergistic effect on the survival rate of the cell, with the AgNPs content being the key factor.
27285857	10	22	theme	AgNPs	1711:1715	arg1	factor					1739:1744	the key factor	1731:1744	the key factor	1731:1744	Film thickness and AgNP content show a synergistic effect on the survival rate of the cell, with the AgNPs content being the key factor.
27285857	9	23	theme	significant	1541:1551	arg1	influence					1553:1561	a more significant influence	1534:1561	a more significant influence on cell adhesion and subsequent proliferation	1534:1607	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	2	24	theme	silver	302:307	arg1	AgNPs					324:328	AgNPs	324:328	AgNPs	324:328	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	2	24	theme	silver	302:307	arg1	nanoparticles					309:321	the doped silver nanoparticles	292:321	the doped silver nanoparticles (AgNPs)	292:329	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	4	25	theme	UV-visible	796:805	arg1	spectrophotometry					807:823	UV-visible spectrophotometry	796:823	UV-visible spectrophotometry	796:823	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	10	26	theme	Film	1610:1613	arg1	thickness					1615:1623	Film thickness	1610:1623	Film thickness	1610:1623	Film thickness and AgNP content show a synergistic effect on the survival rate of the cell, with the AgNPs content being the key factor.
27285857	4	27	theme	electron	769:776	arg1	SEM					790:792	SEM	790:792	SEM	790:792	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	4	27	theme	electron	769:776	arg1	microscopy					778:787	scanning electron microscopy	760:787	scanning electron microscopy (SEM)	760:793	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	2	28	theme	doped	296:300	arg1	AgNPs					324:328	AgNPs	324:328	AgNPs	324:328	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	2	28	theme	doped	296:300	arg1	nanoparticles					309:321	the doped silver nanoparticles	292:321	the doped silver nanoparticles (AgNPs)	292:329	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	9	29	from	influence	1553:1561	arg1	adhesion					1571:1578	cell adhesion	1566:1578	cell adhesion	1566:1578	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	9	29	from	influence	1553:1561	arg1	proliferation					1595:1607	subsequent proliferation	1584:1607	subsequent proliferation	1584:1607	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	2	30	theme	surface	353:359	arg1	properties					361:370	the surface properties	349:370	the surface properties of the matrix	349:384	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	0	31	theme	composite	25:33	arg1	films					35:39	AgNPs/CS composite films	16:39	AgNPs/CS composite films: AgNPs immobilized in chitosan matrix	16:77	Cytotoxicity of AgNPs/CS composite films: AgNPs immobilized in chitosan matrix contributes a higher inhibition rate to cell proliferation.
27285857	4	32	theme	X-ray	735:739	arg1	XRD					754:756	XRD	754:756	XRD	754:756	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	4	32	theme	X-ray	735:739	arg1	diffraction					741:751	X-ray diffraction	735:751	X-ray diffraction (XRD)	735:757	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	0	33	theme	cell	119:122	arg1	proliferation					124:136	cell proliferation	119:136	cell proliferation	119:136	Cytotoxicity of AgNPs/CS composite films: AgNPs immobilized in chitosan matrix contributes a higher inhibition rate to cell proliferation.
27285857	3	34	theme	average	517:523	arg1	size					525:528	average size	517:528	average size	517:528	Here, AgNPs (average size is 25 nm) were doped into chitosan (CS) films by mechanical mixing to form a slow-release system.
27285857	9	35	theme	released	1422:1429	arg1	Ag					1431:1432	the released Ag	1418:1432	the released Ag (either in the peeled debris or dissolved in the fluid)	1418:1488	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	2	36	theme	ion	394:396	arg1	environment					398:408	the ion environment	390:408	the ion environment of the surrounding fluid	390:433	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	0	37	theme	AgNPs/CS	16:23	arg1	films					35:39	AgNPs/CS composite films	16:39	AgNPs/CS composite films: AgNPs immobilized in chitosan matrix	16:77	Cytotoxicity of AgNPs/CS composite films: AgNPs immobilized in chitosan matrix contributes a higher inhibition rate to cell proliferation.
27285857	3	38	theme	mechanical	579:588	arg1	mixing					590:595	mechanical mixing	579:595	mechanical mixing to form a slow-release system	579:625	Here, AgNPs (average size is 25 nm) were doped into chitosan (CS) films by mechanical mixing to form a slow-release system.
27285857	4	39	dep	properties	640:649	arg1	The					628:630	The	628:630	The	628:630	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	1	40	theme	chitosan	200:207	arg1	AgNPs/CS					226:233	AgNPs/CS	226:233	AgNPs/CS	226:233	In this study, the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS) was investigated in vitro.
27285857	1	40	theme	chitosan	200:207	arg1	films					219:223	sliver nanoparticle-doped chitosan composite films	174:223	sliver nanoparticle-doped chitosan composite films (AgNPs/CS)	174:234	In this study, the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS) was investigated in vitro.
27285857	10	41	theme	key	1735:1737	arg1	content					1717:1723	the AgNPs content	1707:1723	the AgNPs content	1707:1723	Film thickness and AgNP content show a synergistic effect on the survival rate of the cell, with the AgNPs content being the key factor.
27285857	10	41	theme	key	1735:1737	arg1	factor					1739:1744	the key factor	1731:1744	the key factor	1731:1744	Film thickness and AgNP content show a synergistic effect on the survival rate of the cell, with the AgNPs content being the key factor.
27285857	5	42	theme	umbilical	955:963	arg1	HUVEC					989:993	HUVEC	989:993	HUVEC	989:993	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	5	42	theme	umbilical	955:963	arg1	cells					982:986	human umbilical vein endothelial cells	949:986	human umbilical vein endothelial cells (HUVEC)	949:994	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	6	43	theme	doped	1111:1115	arg1	AgNPs					1117:1121	the doped AgNPs	1107:1121	the doped AgNPs dispersed in the CS matrix with a polycrystalline structure	1107:1181	Using statistical analysis, the following conclusions can be made: the doped AgNPs dispersed in the CS matrix with a polycrystalline structure.
27285857	1	44	theme	sliver	174:179	arg1	AgNPs/CS					226:233	AgNPs/CS	226:233	AgNPs/CS	226:233	In this study, the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS) was investigated in vitro.
27285857	1	44	theme	sliver	174:179	arg1	films					219:223	sliver nanoparticle-doped chitosan composite films	174:223	sliver nanoparticle-doped chitosan composite films (AgNPs/CS)	174:234	In this study, the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS) was investigated in vitro.
27285857	1	45	theme	composite	209:217	arg1	AgNPs/CS					226:233	AgNPs/CS	226:233	AgNPs/CS	226:233	In this study, the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS) was investigated in vitro.
27285857	1	45	theme	composite	209:217	arg1	films					219:223	sliver nanoparticle-doped chitosan composite films	174:223	sliver nanoparticle-doped chitosan composite films (AgNPs/CS)	174:234	In this study, the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS) was investigated in vitro.
27285857	0	46	theme	films	35:39	arg1	Cytotoxicity					0:11	Cytotoxicity	0:11	Cytotoxicity of AgNPs/CS composite films: AgNPs immobilized in chitosan matrix	0:77	Cytotoxicity of AgNPs/CS composite films: AgNPs immobilized in chitosan matrix contributes a higher inhibition rate to cell proliferation.
27285857	10	47	theme	synergistic	1649:1659	arg1	effect					1661:1666	a synergistic effect	1647:1666	a synergistic effect on the survival rate of the cell	1647:1699	Film thickness and AgNP content show a synergistic effect on the survival rate of the cell, with the AgNPs content being the key factor.
27285857	9	48	theme	AgNPs/CS	1513:1520	arg1	films					1522:1526	the AgNPs/CS films	1509:1526	the AgNPs/CS films	1509:1526	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	5	49	theme	vein	965:968	arg1	HUVEC					989:993	HUVEC	989:993	HUVEC	989:993	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	5	49	theme	vein	965:968	arg1	cells					982:986	human umbilical vein endothelial cells	949:986	human umbilical vein endothelial cells (HUVEC)	949:994	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	9	50	theme	peeled	1449:1454	arg1	debris					1456:1461	the peeled debris	1445:1461	the peeled debris	1445:1461	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	2	51	theme	matrix	379:384	arg1	environment					398:408	the ion environment	390:408	the ion environment of the surrounding fluid	390:433	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	2	51	theme	matrix	379:384	arg1	properties					361:370	the surface properties	349:370	the surface properties of the matrix	349:384	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	10	52	from	effect	1661:1666	arg1	rate					1684:1687	the survival rate	1671:1687	the survival rate of the cell	1671:1699	Film thickness and AgNP content show a synergistic effect on the survival rate of the cell, with the AgNPs content being the key factor.
27285857	5	53	theme	endothelial	970:980	arg1	HUVEC					989:993	HUVEC	989:993	HUVEC	989:993	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	5	53	theme	endothelial	970:980	arg1	cells					982:986	human umbilical vein endothelial cells	949:986	human umbilical vein endothelial cells (HUVEC)	949:994	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	5	54	theme	MTT	924:926	arg1	assays					938:943	both MTT and CCK-8 assays	919:943	assays	938:943	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	2	55	theme	fluid	429:433	arg1	environment					398:408	the ion environment	390:408	the ion environment of the surrounding fluid	390:433	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	2	55	theme	fluid	429:433	arg1	properties					361:370	the surface properties	349:370	the surface properties of the matrix	349:384	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	4	56	theme	weight	832:837	arg1	loss					839:842	a weight loss	830:842	a weight loss method	830:849	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
27285857	5	57	theme	CCK-8	932:936	arg1	assays					938:943	both MTT and CCK-8 assays	919:943	assays	938:943	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	0	58	dep	films	35:39	arg1	AgNPs					42:46	AgNPs	42:46	AgNPs/CS composite films: AgNPs immobilized in chitosan matrix	16:77	Cytotoxicity of AgNPs/CS composite films: AgNPs immobilized in chitosan matrix contributes a higher inhibition rate to cell proliferation.
27285857	8	59	theme	composite	1305:1313	arg1	film					1315:1318	the composite film	1301:1318	the composite film	1301:1318	After that erosion, the composite film became relatively stable, and the doped Ag was slowly released into the fluid.
27285857	6	60	with	matrix	1143:1148	arg1	structure					1173:1181	a polycrystalline structure	1155:1181	a polycrystalline structure	1155:1181	Using statistical analysis, the following conclusions can be made: the doped AgNPs dispersed in the CS matrix with a polycrystalline structure.
27285857	7	61	theme	small	1212:1216	arg1	amount					1218:1223	a small amount	1210:1223	a small amount of debris	1210:1233	During the early erosion, a small amount of debris peeled off and became suspended in the fluid.
27285857	7	61	theme	small	1212:1216	arg1	debris					1228:1233	debris	1228:1233	debris	1228:1233	During the early erosion, a small amount of debris peeled off and became suspended in the fluid.
27285857	2	62	dep	mechanism	478:486	arg1	is					488:489	is	488:489	is	488:489	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	3	63	theme	slow-release	607:618	arg1	system					620:625	a slow-release system	605:625	a slow-release system	605:625	Here, AgNPs (average size is 25 nm) were doped into chitosan (CS) films by mechanical mixing to form a slow-release system.
27285857	5	64	theme	survival	892:899	arg1	rate					901:904	the survival rate	888:904	the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC)	888:994	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	3	65	dep	AgNPs	510:514	arg1	25 nm					533:537	25 nm	533:537	25 nm	533:537	Here, AgNPs (average size is 25 nm) were doped into chitosan (CS) films by mechanical mixing to form a slow-release system.
27285857	5	66	dep	rate	901:904	arg1	obtained					907:914	obtained	907:914	obtained by both MTT and CCK-8 assays	907:943	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	6	67	theme	polycrystalline	1157:1171	arg1	structure					1173:1181	a polycrystalline structure	1155:1181	a polycrystalline structure	1155:1181	Using statistical analysis, the following conclusions can be made: the doped AgNPs dispersed in the CS matrix with a polycrystalline structure.
27285857	5	68	theme	adhered	870:876	arg1	cells					878:882	adhered cells	870:882	adhered cells	870:882	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	9	69	dep	Ag	1431:1432	arg1	either					1435:1440	either	1435:1440	either	1435:1440	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	9	69	dep	Ag	1431:1432	arg1	dissolved					1466:1474	dissolved	1466:1474	dissolved in the fluid	1466:1487	In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation.
27285857	0	70	theme	chitosan	63:70	arg1	matrix					72:77	chitosan matrix	63:77	chitosan matrix	63:77	Cytotoxicity of AgNPs/CS composite films: AgNPs immobilized in chitosan matrix contributes a higher inhibition rate to cell proliferation.
27285857	1	71	theme	films	219:223	arg1	cytotoxicity					158:169	the cytotoxicity	154:169	the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS)	154:234	In this study, the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS) was investigated in vitro.
27285857	3	72	theme	chitosan	556:563	arg1	films					570:574	chitosan (CS) films	556:574	chitosan (CS) films	556:574	Here, AgNPs (average size is 25 nm) were doped into chitosan (CS) films by mechanical mixing to form a slow-release system.
27285857	1	73	theme	nanoparticle-doped	181:198	arg1	AgNPs/CS					226:233	AgNPs/CS	226:233	AgNPs/CS	226:233	In this study, the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS) was investigated in vitro.
27285857	1	73	theme	nanoparticle-doped	181:198	arg1	films					219:223	sliver nanoparticle-doped chitosan composite films	174:223	sliver nanoparticle-doped chitosan composite films (AgNPs/CS)	174:234	In this study, the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS) was investigated in vitro.
27285857	10	74	theme	survival	1675:1682	arg1	rate					1684:1687	the survival rate	1671:1687	the survival rate of the cell	1671:1699	Film thickness and AgNP content show a synergistic effect on the survival rate of the cell, with the AgNPs content being the key factor.
27285857	2	75	theme	dominant	469:476	arg1	mechanism					478:486	the dominant mechanism is	465:489	the dominant mechanism is of interest	465:501	In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest.
27285857	5	76	theme	cells	878:882	arg1	morphology					856:865	The morphology	852:865	The morphology of adhered cells	852:882	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	5	76	theme	cells	878:882	arg1	rate					901:904	the survival rate	888:904	the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC)	888:994	The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity.
27285857	6	77	theme	following	1072:1080	arg1	conclusions					1082:1092	the following conclusions	1068:1092	the following conclusions	1068:1092	Using statistical analysis, the following conclusions can be made: the doped AgNPs dispersed in the CS matrix with a polycrystalline structure.
27285857	10	78	theme	AgNP	1629:1632	arg1	content					1634:1640	AgNP content	1629:1640	AgNP content	1629:1640	Film thickness and AgNP content show a synergistic effect on the survival rate of the cell, with the AgNPs content being the key factor.
27285857	4	79	dep	loss	839:842	arg1	method					844:849	method	844:849	a weight loss method	830:849	The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method.
25530638	2	0	theme	events	517:522	arg1	perturbation					480:491	perturbation	480:491	perturbation of specific recognition events	480:522	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	2	0	theme	events	517:522	arg1	studies					468:474	structure-function studies	449:474	structure-function studies	449:474	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	3	1	from	expression	703:712	arg1	surfaces					751:758	cell surfaces	746:758	cell surfaces	746:758	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	2	2	theme	recognition	505:515	arg1	events					517:522	specific recognition events	496:522	specific recognition events	496:522	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	3	3	theme	fluorinated	717:727	arg1	epitopes					734:741	fluorinated sLex epitopes	717:741	fluorinated sLex epitopes on cell surfaces	717:758	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	3	4	theme	molecular	916:924	arg1	basis					926:930	the molecular basis	912:930	the molecular basis of this reduced adhesion	912:955	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	1	5	theme	broad	248:252	arg1	metastasis					361:370	tumor metastasis	355:370	tumor metastasis	355:370	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	5	theme	broad	248:252	arg1	inflammation					294:305	inflammation	294:305	inflammation	294:305	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	5	theme	broad	248:252	arg1	range					254:258	a broad range	246:258	a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis	246:370	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	5	theme	broad	248:252	arg1	attachment					339:348	microbial attachment	329:348	microbial attachment	329:348	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	5	theme	broad	248:252	arg1	recognition					316:326	antigen recognition	308:326	antigen recognition	308:326	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	0	6	theme	[2+2	96:99	arg1	approach					102:109	a convergent [2+2] approach	83:109	a convergent [2+2] approach	83:109	Total synthesis of trifluorobutyryl-modified, globally protected sialyl Lewis x by a convergent [2+2] approach.
25530638	3	7	theme	sLex	729:732	arg1	epitopes					734:741	fluorinated sLex epitopes	717:741	fluorinated sLex epitopes on cell surfaces	717:758	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	1	8	from	expression	155:164	arg1	surface					197:203	the surface	193:203	the surface of eukaryotic cells	193:223	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	3	9	theme	trifluorobutyryl-modified	561:585	arg1	acid					594:597	a trifluorobutyryl-modified sialic acid	559:597	a trifluorobutyryl-modified sialic acid metabolite	559:608	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	5	10	theme	other	1382:1386	arg1	analogues					1404:1412	other N-modified sLex analogues	1382:1412	other N-modified sLex analogues	1382:1412	This strategy can, in principle, be used for preparation of other N-modified sLex analogues.
25530638	3	11	theme	cell	852:855	arg1	migration.1					857:867	tumor cell migration.1	846:867	tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial	846:1039	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	0	12	theme	convergent	85:94	arg1	approach					102:109	a convergent [2+2] approach	83:109	a convergent [2+2] approach	83:109	Total synthesis of trifluorobutyryl-modified, globally protected sialyl Lewis x by a convergent [2+2] approach.
25530638	1	13	theme	tumor	355:359	arg1	metastasis					361:370	tumor metastasis	355:370	tumor metastasis	355:370	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	14	from	changes	140:146	arg1	surface					197:203	the surface	193:203	the surface of eukaryotic cells	193:223	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	14	from	changes	140:146	arg1	expression					155:164	the expression	151:164	the expression of sialic acid residues on the surface of eukaryotic cells	151:223	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	3	15	theme	sialic	587:592	arg1	acid					594:597	a trifluorobutyryl-modified sialic acid	559:597	a trifluorobutyryl-modified sialic acid metabolite	559:608	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	4	16	theme	protective	1171:1180	arg1	groups					1182:1187	versatile protective groups	1161:1187	versatile protective groups	1161:1187	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	5	17	theme	N-modified	1388:1397	arg1	analogues					1404:1412	other N-modified sLex analogues	1382:1412	other N-modified sLex analogues	1382:1412	This strategy can, in principle, be used for preparation of other N-modified sLex analogues.
25530638	3	18	theme	tumor	846:850	arg1	migration.1					857:867	tumor cell migration.1	846:867	tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial	846:1039	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	3	19	theme	chemical	958:965	arg1	synthesis					967:975	chemical synthesis	958:975	chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex)	958:1027	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	2	20	theme	sialic	401:406	arg1	analogues					413:421	sialic acid analogues	401:421	sialic acid analogues	401:421	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	3	21	theme	cell	746:749	arg1	surfaces					751:758	cell surfaces	746:758	cell surfaces	746:758	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	2	22	theme	structure-function	449:466	arg1	studies					468:474	structure-function studies	449:474	structure-function studies	449:474	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	3	23	theme	reduced	940:946	arg1	adhesion					948:955	this reduced adhesion	935:955	this reduced adhesion	935:955	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	1	24	theme	sialic	169:174	arg1	residues					181:188	sialic acid residues	169:188	sialic acid residues	169:188	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	4	25	theme	efficient	1069:1077	arg1	approach					1085:1092	a highly efficient [2+2] approach	1060:1092	a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups	1060:1187	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	3	26	theme	adhesion	948:955	arg1	basis					926:930	the molecular basis	912:930	the molecular basis of this reduced adhesion	912:955	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	1	27	theme	acid	176:179	arg1	residues					181:188	sialic acid residues	169:188	sialic acid residues	169:188	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	0	28	theme	Total	0:4	arg1	synthesis					6:14	Total synthesis	0:14	Total synthesis of trifluorobutyryl-modified, globally protected sialyl Lewis x by a convergent [2+2] approach.	0:110	Total synthesis of trifluorobutyryl-modified, globally protected sialyl Lewis x by a convergent [2+2] approach.
25530638	1	29	theme	biological	263:272	arg1	metastasis					361:370	tumor metastasis	355:370	tumor metastasis	355:370	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	29	theme	biological	263:272	arg1	inflammation					294:305	inflammation	294:305	inflammation	294:305	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	29	theme	biological	263:272	arg1	recognition					316:326	antigen recognition	308:326	antigen recognition	308:326	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	29	theme	biological	263:272	arg1	processes					274:282	biological processes	263:282	biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis	263:370	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	29	theme	biological	263:272	arg1	attachment					339:348	microbial attachment	329:348	microbial attachment	329:348	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	2	30	from	Uptake	373:378	arg1	cells					436:440	mammalian cells	426:440	mammalian cells	426:440	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	3	31	theme	trifluorobutyrylated	980:999	arg1	C4F3--sLex					1017:1026	C4F3--sLex	1017:1026	C4F3--sLex	1017:1026	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	3	31	theme	trifluorobutyrylated	980:999	arg1	x					1014:1014	trifluorobutyrylated sialyl Lewis x	980:1014	trifluorobutyrylated sialyl Lewis x (C4F3--sLex)	980:1027	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	1	32	theme	residues	181:188	arg1	expression					155:164	the expression	151:164	the expression of sialic acid residues on the surface of eukaryotic cells	151:223	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	0	33	theme	trifluorobutyryl-modified	19:43	arg1	x					78:78	trifluorobutyryl-modified, globally protected sialyl Lewis x	19:78	trifluorobutyryl-modified, globally protected sialyl Lewis x	19:78	Total synthesis of trifluorobutyryl-modified, globally protected sialyl Lewis x by a convergent [2+2] approach.
25530638	1	34	theme	processes	274:282	arg1	metastasis					361:370	tumor metastasis	355:370	tumor metastasis	355:370	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	34	theme	processes	274:282	arg1	inflammation					294:305	inflammation	294:305	inflammation	294:305	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	34	theme	processes	274:282	arg1	range					254:258	a broad range	246:258	a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis	246:370	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	34	theme	processes	274:282	arg1	attachment					339:348	microbial attachment	329:348	microbial attachment	329:348	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	1	34	theme	processes	274:282	arg1	recognition					316:326	antigen recognition	308:326	antigen recognition	308:326	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	3	35	theme	acid	594:597	arg1	metabolite					599:608	a trifluorobutyryl-modified sialic acid metabolite	559:608	a trifluorobutyryl-modified sialic acid metabolite	559:608	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	3	36	theme	sialyl	1001:1006	arg1	C4F3--sLex					1017:1026	C4F3--sLex	1017:1026	C4F3--sLex	1017:1026	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	3	36	theme	sialyl	1001:1006	arg1	x					1014:1014	trifluorobutyrylated sialyl Lewis x	980:1014	trifluorobutyrylated sialyl Lewis x (C4F3--sLex)	980:1027	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	2	37	theme	mammalian	426:434	arg1	cells					436:440	mammalian cells	426:440	mammalian cells	426:440	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	4	38	theme	[2+2	1079:1082	arg1	approach					1085:1092	a highly efficient [2+2] approach	1060:1092	a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups	1060:1187	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	4	39	contain	contains	1152:1159	arg1	approach					1085:1092	a highly efficient [2+2] approach	1060:1092	a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups	1060:1187	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	4	39	contain	contains	1152:1159	arg2	groups					1182:1187	versatile protective groups	1161:1187	versatile protective groups	1161:1187	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	2	40	theme	specific	496:503	arg1	events					517:522	specific recognition events	496:522	specific recognition events	496:522	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	4	41	theme	versatile	1161:1169	arg1	groups					1182:1187	versatile protective groups	1161:1187	versatile protective groups	1161:1187	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	5	42	used	used	1358:1361	arg2	strategy					1327:1334	This strategy	1322:1334	This strategy	1322:1334	This strategy can, in principle, be used for preparation of other N-modified sLex analogues.
25530638	4	43	theme	preparative	1129:1139	arg1	scale					1141:1145	a preparative scale	1127:1145	a preparative scale	1127:1145	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	3	44	theme	mammalian	637:645	arg1	cells					647:651	mammalian cells	637:651	mammalian cells	637:651	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	4	45	dep	surface	1215:1221	arg1	immobilized					1223:1233	immobilized	1223:1233	immobilized	1223:1233	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	4	45	dep	surface	1215:1221	arg1	solubilized					1238:1248	solubilized	1238:1248	solubilized	1238:1248	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	2	46	theme	analogues	413:421	arg1	incorporation					384:396	incorporation	384:396	incorporation	384:396	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	2	46	theme	analogues	413:421	arg1	Uptake					373:378	Uptake	373:378	Uptake	373:378	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	3	47	theme	cells	647:651	arg1	adhesion					625:632	the adhesion	621:632	the adhesion of mammalian cells to E and P-selectin	621:671	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	1	48	from	surface	197:203	arg1	changes					140:146	Structural and quantitative changes	112:146	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells	112:223	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	2	49	theme	acid	408:411	arg1	analogues					413:421	sialic acid analogues	401:421	sialic acid analogues	401:421	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	0	50	theme	sialyl	65:70	arg1	x					78:78	trifluorobutyryl-modified, globally protected sialyl Lewis x	19:78	trifluorobutyryl-modified, globally protected sialyl Lewis x	19:78	Total synthesis of trifluorobutyryl-modified, globally protected sialyl Lewis x by a convergent [2+2] approach.
25530638	1	51	theme	antigen	308:314	arg1	recognition					316:326	antigen recognition	308:326	antigen recognition	308:326	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	3	52	theme	sLex-selectin	786:798	arg1	interactions					800:811	the sLex-selectin interactions	782:811	the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial	782:1039	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	3	53	theme	epitopes	734:741	arg1	expression					703:712	the expression	699:712	the expression of fluorinated sLex epitopes on cell surfaces	699:758	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	3	54	dep	migration.1	857:867	arg1	crucial					1033:1039	crucial	1033:1039	crucial	1033:1039	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	3	55	theme	Lewis	1008:1012	arg1	C4F3--sLex					1017:1026	C4F3--sLex	1017:1026	C4F3--sLex	1017:1026	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	3	55	theme	Lewis	1008:1012	arg1	x					1014:1014	trifluorobutyrylated sialyl Lewis x	980:1014	trifluorobutyrylated sialyl Lewis x (C4F3--sLex)	980:1027	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	1	56	theme	Structural	112:121	arg1	changes					140:146	Structural and quantitative changes	112:146	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells	112:223	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	4	57	theme	C4F3-sLex	1114:1122	arg1	assembly					1102:1109	the assembly	1098:1109	the assembly of C4F3-sLex on a preparative scale	1098:1145	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	3	58	from	surfaces	751:758	arg1	expression					703:712	the expression	699:712	the expression of fluorinated sLex epitopes on cell surfaces	699:758	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	0	59	dep	trifluorobutyryl-modified	19:43	arg1	protected					55:63	protected	55:63	protected	55:63	Total synthesis of trifluorobutyryl-modified, globally protected sialyl Lewis x by a convergent [2+2] approach.
25530638	3	60	theme	x	1014:1014	arg1	synthesis					967:975	chemical synthesis	958:975	chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex)	958:1027	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	3	61	from	epitopes	734:741	arg1	surfaces					751:758	cell surfaces	746:758	cell surfaces	746:758	Our group has recently shown that a trifluorobutyryl-modified sialic acid metabolite diminishes the adhesion of mammalian cells to E and P-selectin, presumably by leading to the expression of fluorinated sLex epitopes on cell surfaces, and interfering with the sLex-selectin interactions that are well known in mediating tumor cell migration.1 For studies directed towards understanding the molecular basis of this reduced adhesion, chemical synthesis of trifluorobutyrylated sialyl Lewis x (C4F3--sLex) was crucial.
25530638	1	62	theme	eukaryotic	208:217	arg1	cells					219:223	eukaryotic cells	208:223	eukaryotic cells	208:223	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	2	63	from	incorporation	384:396	arg1	cells					436:440	mammalian cells	426:440	mammalian cells	426:440	Uptake and incorporation of sialic acid analogues in mammalian cells enable structure-function studies and perturbation of specific recognition events.
25530638	5	64	theme	sLex	1399:1402	arg1	analogues					1404:1412	other N-modified sLex analogues	1382:1412	other N-modified sLex analogues	1382:1412	This strategy can, in principle, be used for preparation of other N-modified sLex analogues.
25530638	1	65	theme	quantitative	127:138	arg1	changes					140:146	Structural and quantitative changes	112:146	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells	112:223	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	4	66	theme	selectin	1299:1306	arg1	interactions					1308:1319	selectin interactions	1299:1319	selectin interactions	1299:1319	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	1	67	theme	cells	219:223	arg1	surface					197:203	the surface	193:203	the surface of eukaryotic cells	193:223	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	0	68	theme	x	78:78	arg1	synthesis					6:14	Total synthesis	0:14	Total synthesis of trifluorobutyryl-modified, globally protected sialyl Lewis x by a convergent [2+2] approach.	0:110	Total synthesis of trifluorobutyryl-modified, globally protected sialyl Lewis x by a convergent [2+2] approach.
25530638	5	69	theme	analogues	1404:1412	arg1	preparation					1367:1377	preparation	1367:1377	preparation of other N-modified sLex analogues	1367:1412	This strategy can, in principle, be used for preparation of other N-modified sLex analogues.
25530638	4	70	from	assembly	1102:1109	arg1	scale					1141:1145	a preparative scale	1127:1145	a preparative scale	1127:1145	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25530638	0	71	theme	Lewis	72:76	arg1	x					78:78	trifluorobutyryl-modified, globally protected sialyl Lewis x	19:78	trifluorobutyryl-modified, globally protected sialyl Lewis x	19:78	Total synthesis of trifluorobutyryl-modified, globally protected sialyl Lewis x by a convergent [2+2] approach.
25530638	1	72	theme	microbial	329:337	arg1	attachment					339:348	microbial attachment	329:348	microbial attachment	329:348	Structural and quantitative changes in the expression of sialic acid residues on the surface of eukaryotic cells profoundly influence a broad range of biological processes including inflammation, antigen recognition, microbial attachment, and tumor metastasis.
25530638	4	73	theme	biophysical	1264:1274	arg1	studies					1276:1282	biophysical studies to investigate selectin interactions	1264:1319	biophysical studies to investigate selectin interactions	1264:1319	We have developed a highly efficient [2+2] approach for the assembly of C4F3-sLex on a preparative scale that contains versatile protective groups allowing the glycan to be surface immobilized or solubilized as needed for biophysical studies to investigate selectin interactions.
25445196	9	0	theme	intensity	1857:1865	arg1	differences					1867:1877	intensity differences	1857:1877	intensity differences	1857:1877	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	8	1	dep	intensity	1667:1675	arg1	The					1654:1656	The	1654:1656	The	1654:1656	The staining intensity and distribution of ECM molecules revealed a number of differences between the functionally different subnuclei of IO.
25445196	8	2	theme	staining	1658:1665	arg1	intensity					1667:1675	staining intensity	1658:1675	staining intensity	1658:1675	The staining intensity and distribution of ECM molecules revealed a number of differences between the functionally different subnuclei of IO.
25445196	1	3	theme	diffuse	269:275	arg1	form					287:290	diffuse reticular form	269:290	diffuse reticular form throughout the neuropil	269:314	Extracellular matrix (ECM) accumulates around different neuronal compartments of the central nervous system (CNS) or appears in diffuse reticular form throughout the neuropil.
25445196	9	4	theme	reaction	1886:1893	arg1	composition					1841:1851	different molecular composition	1821:1851	different molecular composition	1821:1851	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	9	4	theme	reaction	1886:1893	arg1	differences					1867:1877	intensity differences	1857:1877	intensity differences	1857:1877	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	3	5	theme	ECM	683:685	arg1	composition					668:678	molecular composition	658:678	molecular composition	658:678	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	3	5	theme	ECM	683:685	arg1	distribution					641:652	heterogeneous distribution	627:652	heterogeneous distribution	627:652	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	1	6	theme	different	187:195	arg1	compartments					206:217	different neuronal compartments	187:217	different neuronal compartments of the central nervous system (CNS)	187:253	Extracellular matrix (ECM) accumulates around different neuronal compartments of the central nervous system (CNS) or appears in diffuse reticular form throughout the neuropil.
25445196	6	7	theme	glomeruli	1473:1481	arg1	terminals					1443:1451	the axon terminals	1434:1451	the axon terminals of olivary synaptic glomeruli	1434:1481	On the other hand, the darkly stained dots or ring-like structures in the neuropil might represent the periaxonal coats around the axon terminals of olivary synaptic glomeruli.
25445196	3	8	from	functions	832:840	arg1	mechanisms					901:910	the compensatory mechanisms	884:910	the compensatory mechanisms following vestibular lesion	884:938	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	3	8	from	functions	832:840	arg1	gaze					845:848	gaze	845:848	gaze	845:848	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	9	9	theme	different	1914:1922	arg1	mechanisms					1932:1941	different control mechanisms	1914:1941	different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO	1914:2058	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	8	10	theme	molecules	1701:1709	arg1	intensity					1667:1675	staining intensity	1658:1675	staining intensity	1658:1675	The staining intensity and distribution of ECM molecules revealed a number of differences between the functionally different subnuclei of IO.
25445196	8	10	theme	molecules	1701:1709	arg1	distribution					1681:1692	distribution	1681:1692	distribution	1681:1692	The staining intensity and distribution of ECM molecules revealed a number of differences between the functionally different subnuclei of IO.
25445196	4	11	theme	inferior	1023:1030	arg1	IO					1039:1040	IO	1039:1040	IO	1039:1040	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	4	11	theme	inferior	1023:1030	arg1	olive					1032:1036	the climbing fiber-generating inferior olive	993:1036	the climbing fiber-generating inferior olive (IO)	993:1041	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	4	11	theme	inferior	1023:1030	arg1	related					1066:1072	related	1066:1072	related	1066:1072	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	1	12	theme	nervous	234:240	arg1	CNS					250:252	CNS	250:252	CNS	250:252	Extracellular matrix (ECM) accumulates around different neuronal compartments of the central nervous system (CNS) or appears in diffuse reticular form throughout the neuropil.
25445196	1	12	theme	nervous	234:240	arg1	system					242:247	the central nervous system	222:247	the central nervous system (CNS)	222:253	Extracellular matrix (ECM) accumulates around different neuronal compartments of the central nervous system (CNS) or appears in diffuse reticular form throughout the neuropil.
25445196	7	13	theme	proteoglycan	1570:1581	arg1	protein					1588:1594	proteoglycan link protein 1	1570:1596	proteoglycan link protein 1	1570:1596	We have observed positive ECM reaction for the hyaluronan, tenascin-R, hyaluronan and proteoglycan link protein 1 (HAPLN1) and various chondroitin sulfate proteoglycans.
25445196	5	14	from	perikarya	1259:1267	arg1	absence					1232:1238	the absence	1228:1238	the absence of synapses on the perikarya and proximal dendrites of IO neurons	1228:1304	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	9	15	theme	gaze	1946:1949	arg1	mechanisms					1932:1941	different control mechanisms	1914:1941	different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO	1914:2058	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	3	16	theme	hodological	740:750	arg1	organization					752:763	hodological organization	740:763	hodological organization	740:763	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	9	17	theme	posture	1955:1961	arg1	mechanisms					1932:1941	different control mechanisms	1914:1941	different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO	1914:2058	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	5	18	theme	synapses	1243:1250	arg1	absence					1232:1238	the absence	1228:1238	the absence of synapses on the perikarya and proximal dendrites of IO neurons	1228:1304	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	6	19	from	structures	1363:1372	arg1	neuropil					1381:1388	the neuropil	1377:1388	the neuropil	1377:1388	On the other hand, the darkly stained dots or ring-like structures in the neuropil might represent the periaxonal coats around the axon terminals of olivary synaptic glomeruli.
25445196	3	20	theme	vestibular	772:781	arg1	nuclei					783:788	the vestibular nuclei	768:788	the vestibular nuclei	768:788	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	4	21	theme	fiber-generating	1006:1021	arg1	IO					1039:1040	IO	1039:1040	IO	1039:1040	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	4	21	theme	fiber-generating	1006:1021	arg1	olive					1032:1036	the climbing fiber-generating inferior olive	993:1036	the climbing fiber-generating inferior olive (IO)	993:1041	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	4	21	theme	fiber-generating	1006:1021	arg1	related					1066:1072	related	1066:1072	related	1066:1072	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	7	22	theme	sulfate	1631:1637	arg1	proteoglycans					1639:1651	various chondroitin sulfate proteoglycans	1611:1651	various chondroitin sulfate proteoglycans	1611:1651	We have observed positive ECM reaction for the hyaluronan, tenascin-R, hyaluronan and proteoglycan link protein 1 (HAPLN1) and various chondroitin sulfate proteoglycans.
25445196	0	23	theme	fiber-generating	81:96	arg1	olive					134:138	the inferior olive	121:138	the inferior olive	121:138	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	0	23	theme	fiber-generating	81:96	arg1	nucleus					112:118	the climbing fiber-generating precerebellar nucleus	68:118	the climbing fiber-generating precerebellar nucleus	68:118	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	3	24	dep	cytoarchitecture	719:734	arg1	the					706:708	the	706:708	the	706:708	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	6	25	theme	other	1314:1318	arg1	hand					1320:1323	the other hand	1310:1323	the other hand	1310:1323	On the other hand, the darkly stained dots or ring-like structures in the neuropil might represent the periaxonal coats around the axon terminals of olivary synaptic glomeruli.
25445196	8	26	theme	IO	1792:1793	arg1	subnuclei					1779:1787	the functionally different subnuclei	1752:1787	the functionally different subnuclei of IO	1752:1793	The staining intensity and distribution of ECM molecules revealed a number of differences between the functionally different subnuclei of IO.
25445196	7	27	theme	ECM	1510:1512	arg1	reaction					1514:1521	positive ECM reaction	1501:1521	positive ECM reaction for the hyaluronan, tenascin-R, hyaluronan and proteoglycan link protein 1 (HAPLN1) and various chondroitin sulfate proteoglycans	1501:1651	We have observed positive ECM reaction for the hyaluronan, tenascin-R, hyaluronan and proteoglycan link protein 1 (HAPLN1) and various chondroitin sulfate proteoglycans.
25445196	2	28	theme	individual	485:494	arg1	synapses					496:503	the individual synapses	481:503	the individual synapses	481:503	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	5	29	theme	IO	1295:1296	arg1	neurons					1298:1304	IO neurons	1295:1304	IO neurons	1295:1304	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	3	30	dep	distribution	641:652	arg1	the					623:625	the	623:625	the	623:625	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	3	31	dep	gaze	845:848	arg1	control					862:868	control	862:868	control	862:868	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	7	32	theme	chondroitin	1619:1629	arg1	proteoglycans					1639:1651	various chondroitin sulfate proteoglycans	1611:1651	various chondroitin sulfate proteoglycans	1611:1651	We have observed positive ECM reaction for the hyaluronan, tenascin-R, hyaluronan and proteoglycan link protein 1 (HAPLN1) and various chondroitin sulfate proteoglycans.
25445196	4	33	theme	climbing	997:1004	arg1	IO					1039:1040	IO	1039:1040	IO	1039:1040	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	4	33	theme	climbing	997:1004	arg1	olive					1032:1036	the climbing fiber-generating inferior olive	993:1036	the climbing fiber-generating inferior olive (IO)	993:1041	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	4	33	theme	climbing	997:1004	arg1	related					1066:1072	related	1066:1072	related	1066:1072	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	5	34	theme	characteristic	1165:1178	arg1	finding					1180:1186	the most characteristic finding	1156:1186	the most characteristic finding	1156:1186	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	5	34	theme	characteristic	1165:1178	arg1	lack					1196:1199	the lack	1192:1199	the lack of PNNs	1192:1207	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	0	35	theme	climbing	72:79	arg1	olive					134:138	the inferior olive	121:138	the inferior olive	121:138	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	0	35	theme	climbing	72:79	arg1	nucleus					112:118	the climbing fiber-generating precerebellar nucleus	68:118	the climbing fiber-generating precerebellar nucleus	68:118	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	0	36	theme	Extracellular	0:12	arg1	molecules					21:29	Extracellular matrix molecules	0:29	Extracellular matrix molecules	0:29	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	1	37	theme	reticular	277:285	arg1	form					287:290	diffuse reticular form	269:290	diffuse reticular form throughout the neuropil	269:314	Extracellular matrix (ECM) accumulates around different neuronal compartments of the central nervous system (CNS) or appears in diffuse reticular form throughout the neuropil.
25445196	2	38	theme	types	418:422	arg1	dendrites					390:398	dendrites	390:398	dendrites	390:398	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	2	38	theme	types	418:422	arg1	perikarya					376:384	perikarya	376:384	perikarya	376:384	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	2	39	theme	various	403:409	arg1	types					418:422	various neuron types	403:422	various neuron types	403:422	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	4	40	theme	expression	971:980	arg1	pattern					982:988	the ECM expression pattern	963:988	the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei	963:1097	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	9	41	theme	control	1924:1930	arg1	mechanisms					1932:1941	different control mechanisms	1914:1941	different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO	1914:2058	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	6	42	theme	ring-like	1353:1361	arg1	structures					1363:1372	ring-like structures	1353:1372	ring-like structures in the neuropil	1353:1388	On the other hand, the darkly stained dots or ring-like structures in the neuropil might represent the periaxonal coats around the axon terminals of olivary synaptic glomeruli.
25445196	5	43	theme	histochemical	1109:1121	arg1	methods					1147:1153	histochemical and immunohistochemical methods	1109:1153	histochemical and immunohistochemical methods	1109:1153	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	0	44	theme	unique	39:44	arg1	pattern					57:63	unique expression pattern	39:63	unique expression pattern	39:63	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	2	45	theme	axonal	437:442	arg1	aggregations					454:465	aggregations	454:465	aggregations of ECM around the individual synapses	454:503	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	2	45	theme	axonal	437:442	arg1	coats					444:448	the axonal coats	433:448	the axonal coats	433:448	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	5	46	theme	immunohistochemical	1127:1145	arg1	methods					1147:1153	histochemical and immunohistochemical methods	1109:1153	histochemical and immunohistochemical methods	1109:1153	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	3	47	theme	vestibular	922:931	arg1	lesion					933:938	vestibular lesion	922:938	vestibular lesion	922:938	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	6	48	theme	axon	1438:1441	arg1	terminals					1443:1451	the axon terminals	1434:1451	the axon terminals of olivary synaptic glomeruli	1434:1481	On the other hand, the darkly stained dots or ring-like structures in the neuropil might represent the periaxonal coats around the axon terminals of olivary synaptic glomeruli.
25445196	1	49	theme	system	242:247	arg1	compartments					206:217	different neuronal compartments	187:217	different neuronal compartments of the central nervous system (CNS)	187:253	Extracellular matrix (ECM) accumulates around different neuronal compartments of the central nervous system (CNS) or appears in diffuse reticular form throughout the neuropil.
25445196	9	50	theme	molecular	1831:1839	arg1	composition					1841:1851	different molecular composition	1821:1851	different molecular composition	1821:1851	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	2	51	dep	perikarya	376:384	arg1	the					372:374	the	372:374	the	372:374	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	5	52	dep	perikarya	1259:1267	arg1	the					1255:1257	the	1255:1257	the	1255:1257	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	6	53	theme	periaxonal	1410:1419	arg1	coats					1421:1425	the periaxonal coats	1406:1425	the periaxonal coats around the axon terminals of olivary synaptic glomeruli	1406:1481	On the other hand, the darkly stained dots or ring-like structures in the neuropil might represent the periaxonal coats around the axon terminals of olivary synaptic glomeruli.
25445196	2	54	theme	Ranvier	553:559	arg1	nodes					544:548	the nodes	540:548	the nodes of Ranvier	540:559	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	3	55	theme	molecular	658:666	arg1	composition					668:678	molecular composition	658:678	molecular composition	658:678	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	5	56	from	absence	1232:1238	arg1	perikarya					1259:1267	perikarya	1259:1267	perikarya	1259:1267	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	5	56	from	absence	1232:1238	arg1	dendrites					1282:1290	proximal dendrites	1273:1290	proximal dendrites	1273:1290	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	9	57	theme	ECM	1882:1884	arg1	reaction					1886:1893	ECM reaction	1882:1893	ECM reaction	1882:1893	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	9	58	dep	gaze	1946:1949	arg1	control					1963:1969	control	1963:1969	control	1963:1969	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	1	59	theme	neuronal	197:204	arg1	compartments					206:217	different neuronal compartments	187:217	different neuronal compartments of the central nervous system (CNS)	187:253	Extracellular matrix (ECM) accumulates around different neuronal compartments of the central nervous system (CNS) or appears in diffuse reticular form throughout the neuropil.
25445196	6	60	theme	synaptic	1464:1471	arg1	glomeruli					1473:1481	olivary synaptic glomeruli	1456:1481	olivary synaptic glomeruli	1456:1481	On the other hand, the darkly stained dots or ring-like structures in the neuropil might represent the periaxonal coats around the axon terminals of olivary synaptic glomeruli.
25445196	7	61	theme	link	1583:1586	arg1	protein					1588:1594	proteoglycan link protein 1	1570:1596	proteoglycan link protein 1	1570:1596	We have observed positive ECM reaction for the hyaluronan, tenascin-R, hyaluronan and proteoglycan link protein 1 (HAPLN1) and various chondroitin sulfate proteoglycans.
25445196	2	62	theme	nodal	514:518	arg1	ECM					520:522	the nodal ECM	510:522	the nodal ECM	510:522	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	5	63	from	synapses	1243:1250	arg1	perikarya					1259:1267	perikarya	1259:1267	perikarya	1259:1267	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	5	63	from	synapses	1243:1250	arg1	dendrites					1282:1290	proximal dendrites	1273:1290	proximal dendrites	1273:1290	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	4	64	theme	vestibular	1081:1090	arg1	nuclei					1092:1097	the vestibular nuclei	1077:1097	the vestibular nuclei	1077:1097	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	3	65	theme	variable	710:717	arg1	cytoarchitecture					719:734	variable cytoarchitecture	710:734	variable cytoarchitecture	710:734	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	8	66	theme	ECM	1697:1699	arg1	molecules					1701:1709	ECM molecules	1697:1709	ECM molecules	1697:1709	The staining intensity and distribution of ECM molecules revealed a number of differences between the functionally different subnuclei of IO.
25445196	1	67	theme	central	226:232	arg1	CNS					250:252	CNS	250:252	CNS	250:252	Extracellular matrix (ECM) accumulates around different neuronal compartments of the central nervous system (CNS) or appears in diffuse reticular form throughout the neuropil.
25445196	1	67	theme	central	226:232	arg1	system					242:247	the central nervous system	222:247	the central nervous system (CNS)	222:253	Extracellular matrix (ECM) accumulates around different neuronal compartments of the central nervous system (CNS) or appears in diffuse reticular form throughout the neuropil.
25445196	5	68	from	dendrites	1282:1290	arg1	absence					1232:1238	the absence	1228:1238	the absence of synapses on the perikarya and proximal dendrites of IO neurons	1228:1304	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	6	69	from	dots	1345:1348	arg1	neuropil					1381:1388	the neuropil	1377:1388	the neuropil	1377:1388	On the other hand, the darkly stained dots or ring-like structures in the neuropil might represent the periaxonal coats around the axon terminals of olivary synaptic glomeruli.
25445196	9	70	dep	composition	1841:1851	arg1	the					1817:1819	the	1817:1819	the	1817:1819	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	1	71	theme	Extracellular	141:153	arg1	ECM					163:165	ECM	163:165	ECM	163:165	Extracellular matrix (ECM) accumulates around different neuronal compartments of the central nervous system (CNS) or appears in diffuse reticular form throughout the neuropil.
25445196	1	71	theme	Extracellular	141:153	arg1	matrix					155:160	Extracellular matrix	141:160	Extracellular matrix (ECM)	141:166	Extracellular matrix (ECM) accumulates around different neuronal compartments of the central nervous system (CNS) or appears in diffuse reticular form throughout the neuropil.
25445196	6	72	theme	stained	1337:1343	arg1	dots					1345:1348	the darkly stained dots	1326:1348	the darkly stained dots	1326:1348	On the other hand, the darkly stained dots or ring-like structures in the neuropil might represent the periaxonal coats around the axon terminals of olivary synaptic glomeruli.
25445196	3	73	theme	Previous	562:569	arg1	studies					571:577	Previous studies	562:577	Previous studies in our laboratory	562:595	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	8	74	theme	differences	1732:1742	arg1	number					1722:1727	a number	1720:1727	a number of differences between the functionally different subnuclei of IO	1720:1793	The staining intensity and distribution of ECM molecules revealed a number of differences between the functionally different subnuclei of IO.
25445196	3	75	theme	specific	823:830	arg1	functions					832:840	their specific functions	817:840	their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion	817:938	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	2	76	theme	ECM	470:472	arg1	aggregations					454:465	aggregations	454:465	aggregations of ECM around the individual synapses	454:503	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	2	76	theme	ECM	470:472	arg1	coats					444:448	the axonal coats	433:448	the axonal coats	433:448	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	3	77	theme	nuclei	783:788	arg1	cytoarchitecture					719:734	variable cytoarchitecture	710:734	variable cytoarchitecture	710:734	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	3	77	theme	nuclei	783:788	arg1	organization					752:763	hodological organization	740:763	hodological organization	740:763	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	0	78	theme	precerebellar	98:110	arg1	olive					134:138	the inferior olive	121:138	the inferior olive	121:138	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	0	78	theme	precerebellar	98:110	arg1	nucleus					112:118	the climbing fiber-generating precerebellar nucleus	68:118	the climbing fiber-generating precerebellar nucleus	68:118	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	9	79	theme	visuomotor-vestibular	1987:2007	arg1	subnuclei					2040:2048	the visuomotor-vestibular, somatosensory and integrative subnuclei	1983:2048	the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO	1983:2058	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	8	80	theme	different	1769:1777	arg1	subnuclei					1779:1787	the functionally different subnuclei	1752:1787	the functionally different subnuclei of IO	1752:1793	The staining intensity and distribution of ECM molecules revealed a number of differences between the functionally different subnuclei of IO.
25445196	7	81	theme	positive	1501:1508	arg1	reaction					1514:1521	positive ECM reaction	1501:1521	positive ECM reaction for the hyaluronan, tenascin-R, hyaluronan and proteoglycan link protein 1 (HAPLN1) and various chondroitin sulfate proteoglycans	1501:1651	We have observed positive ECM reaction for the hyaluronan, tenascin-R, hyaluronan and proteoglycan link protein 1 (HAPLN1) and various chondroitin sulfate proteoglycans.
25445196	9	82	theme	somatosensory	2010:2022	arg1	subnuclei					2040:2048	the visuomotor-vestibular, somatosensory and integrative subnuclei	1983:2048	the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO	1983:2058	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	6	83	theme	olivary	1456:1462	arg1	glomeruli					1473:1481	olivary synaptic glomeruli	1456:1481	olivary synaptic glomeruli	1456:1481	On the other hand, the darkly stained dots or ring-like structures in the neuropil might represent the periaxonal coats around the axon terminals of olivary synaptic glomeruli.
25445196	0	84	theme	inferior	125:132	arg1	olive					134:138	the inferior olive	121:138	the inferior olive	121:138	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	0	84	theme	inferior	125:132	arg1	nucleus					112:118	the climbing fiber-generating precerebellar nucleus	68:118	the climbing fiber-generating precerebellar nucleus	68:118	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	9	85	theme	integrative	2028:2038	arg1	subnuclei					2040:2048	the visuomotor-vestibular, somatosensory and integrative subnuclei	1983:2048	the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO	1983:2058	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	5	86	theme	neurons	1298:1304	arg1	perikarya					1259:1267	perikarya	1259:1267	perikarya	1259:1267	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	5	86	theme	neurons	1298:1304	arg1	dendrites					1282:1290	proximal dendrites	1273:1290	proximal dendrites	1273:1290	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	0	87	theme	matrix	14:19	arg1	molecules					21:29	Extracellular matrix molecules	0:29	Extracellular matrix molecules	0:29	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	2	88	located	localized	527:535	arg1	nodes					544:548	the nodes	540:548	the nodes of Ranvier	540:559	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	2	88	located	localized	527:535	arg2	ECM					520:522	the nodal ECM	510:522	the nodal ECM	510:522	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	7	89	theme	various	1611:1617	arg1	proteoglycans					1639:1651	various chondroitin sulfate proteoglycans	1611:1651	various chondroitin sulfate proteoglycans	1611:1651	We have observed positive ECM reaction for the hyaluronan, tenascin-R, hyaluronan and proteoglycan link protein 1 (HAPLN1) and various chondroitin sulfate proteoglycans.
25445196	2	90	theme	neuron	411:416	arg1	types					418:422	various neuron types	403:422	various neuron types	403:422	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	9	91	theme	IO	2057:2058	arg1	subnuclei					2040:2048	the visuomotor-vestibular, somatosensory and integrative subnuclei	1983:2048	the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO	1983:2058	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	5	92	theme	proximal	1273:1280	arg1	dendrites					1282:1290	proximal dendrites	1273:1290	proximal dendrites	1273:1290	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	0	93	theme	expression	46:55	arg1	pattern					57:63	unique expression pattern	39:63	unique expression pattern	39:63	Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive.
25445196	3	94	from	studies	571:577	arg1	laboratory					586:595	our laboratory	582:595	our laboratory	582:595	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	3	95	theme	compensatory	888:899	arg1	mechanisms					901:910	the compensatory mechanisms	884:910	the compensatory mechanisms following vestibular lesion	884:938	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	4	96	theme	ECM	967:969	arg1	pattern					982:988	the ECM expression pattern	963:988	the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei	963:1097	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	4	97	from	pattern	982:988	arg1	IO					1039:1040	IO	1039:1040	IO	1039:1040	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	4	97	from	pattern	982:988	arg1	olive					1032:1036	the climbing fiber-generating inferior olive	993:1036	the climbing fiber-generating inferior olive (IO)	993:1041	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	4	97	from	pattern	982:988	arg1	related					1066:1072	related	1066:1072	related	1066:1072	Here, we investigated the ECM expression pattern in the climbing fiber-generating inferior olive (IO), which is functionally related to the vestibular nuclei.
25445196	3	98	theme	heterogeneous	627:639	arg1	distribution					641:652	heterogeneous distribution	627:652	heterogeneous distribution	627:652	Previous studies in our laboratory demonstrated on rats that the heterogeneous distribution and molecular composition of ECM is associated with the variable cytoarchitecture and hodological organization of the vestibular nuclei and may also be related to their specific functions in gaze and posture control as well as in the compensatory mechanisms following vestibular lesion.
25445196	9	99	theme	different	1821:1829	arg1	composition					1841:1851	different molecular composition	1821:1851	different molecular composition	1821:1851	We hypothesized that the different molecular composition and intensity differences of ECM reaction is associated with different control mechanisms of gaze and posture control executed by the visuomotor-vestibular, somatosensory and integrative subnuclei of the IO.
25445196	2	100	theme	adult	324:328	arg1	CNS					330:332	the adult CNS	320:332	the adult CNS	320:332	In the adult CNS, the perineuronal net (PNN) surrounds the perikarya and dendrites of various neuron types, whereas the axonal coats are aggregations of ECM around the individual synapses, and the nodal ECM is localized at the nodes of Ranvier.
25445196	5	101	theme	PNNs	1204:1207	arg1	finding					1180:1186	the most characteristic finding	1156:1186	the most characteristic finding	1156:1186	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25445196	5	101	theme	PNNs	1204:1207	arg1	lack					1196:1199	the lack	1192:1199	the lack of PNNs	1192:1207	By using histochemical and immunohistochemical methods, the most characteristic finding was the lack of PNNs, presumably due to the absence of synapses on the perikarya and proximal dendrites of IO neurons.
25590696	0	0	theme	wood	87:90	arg1	nanostructures					92:105	strong, self-healing, and transparent artificial wood nanostructures	38:105	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.	0:106	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.
25590696	3	1	theme	layer	676:680	arg1	pair					682:685	layer pair	676:685	layer pair	676:685	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	9	2	theme	water	1662:1666	arg1	effect					1652:1657	the plastifying effect	1636:1657	the plastifying effect of water	1636:1666	We attribute the switchability of the mechanical properties and the propensity for self-repair to changes in the polycation mobility that are brought about by the plastifying effect of water.
25590696	9	3	theme	properties	1526:1535	arg1	propensity					1545:1554	the propensity	1541:1554	the propensity for self-repair	1541:1570	We attribute the switchability of the mechanical properties and the propensity for self-repair to changes in the polycation mobility that are brought about by the plastifying effect of water.
25590696	9	3	theme	properties	1526:1535	arg1	switchability					1494:1506	the switchability	1490:1506	the switchability of the mechanical properties	1490:1535	We attribute the switchability of the mechanical properties and the propensity for self-repair to changes in the polycation mobility that are brought about by the plastifying effect of water.
25590696	9	4	theme	plastifying	1640:1650	arg1	effect					1652:1657	the plastifying effect	1636:1657	the plastifying effect of water	1636:1666	We attribute the switchability of the mechanical properties and the propensity for self-repair to changes in the polycation mobility that are brought about by the plastifying effect of water.
25590696	0	5	theme	artificial	76:85	arg1	nanostructures					92:105	strong, self-healing, and transparent artificial wood nanostructures	38:105	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.	0:106	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.
25590696	7	6	from	break	1377:1381	arg1	strain					1367:1372	strain	1367:1372	strain at break	1367:1381	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	9	7	theme	mechanical	1515:1524	arg1	properties					1526:1535	the mechanical properties	1511:1535	the mechanical properties	1511:1535	We attribute the switchability of the mechanical properties and the propensity for self-repair to changes in the polycation mobility that are brought about by the plastifying effect of water.
25590696	1	8	contain	possessing	128:137	arg2	biodegradability					241:256	partial biodegradability	233:256	partial biodegradability	233:256	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	8	contain	possessing	128:137	arg1	films					122:126	Nanocomposite films	108:126	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability)	108:257	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	8	contain	possessing	128:137	arg2	properties					160:169	multiple interesting properties	139:169	multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability)	139:257	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	8	contain	possessing	128:137	arg2	transparency					201:212	optical transparency	193:212	optical transparency	193:212	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	8	contain	possessing	128:137	arg2	self-healing					215:226	self-healing	215:226	self-healing	215:226	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	8	contain	possessing	128:137	arg2	strength					183:190	mechanical strength	172:190	mechanical strength	172:190	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	6	9	theme	water	1134:1138	arg1	effect					1124:1129	the plasticizing effect	1107:1129	the plasticizing effect of water in the films	1107:1151	Experiments at different humidities revealed the plasticizing effect of water in the films and allowed reversible switching of their mechanical properties.
25590696	4	10	from	11	715:716	arg1	range					699:703	the pH range	692:703	the pH range from 8 to 11	692:716	In the pH range from 8 to 11, micron thick and optically transparent LbL films are obtained by automated dipping when dried regularly in a stream of air.
25590696	2	11	theme	thick	324:328	arg1	films					344:348	micron thick wood-inspired films	317:348	micron thick wood-inspired films	317:348	We used Layer-by-Layer assembly to prepare micron thick wood-inspired films from anionic nanofibrillated cellulose and cationic poly(vinyl amine).
25590696	5	12	theme	cm	867:868	arg1	size					856:859	a size	854:859	a size of 10 cm(2) or more	854:879	Films with a size of 10 cm(2) or more can be peeled from flat surfaces; they show tensile strengths up to about 250 MPa and Young's moduli up to about 18 GPa as controlled by the polycation/polyanion ratio of the film.
25590696	5	13	theme	film	1056:1059	arg1	ratio					1043:1047	the polycation/polyanion ratio	1018:1047	the polycation/polyanion ratio of the film	1018:1059	Films with a size of 10 cm(2) or more can be peeled from flat surfaces; they show tensile strengths up to about 250 MPa and Young's moduli up to about 18 GPa as controlled by the polycation/polyanion ratio of the film.
25590696	4	14	theme	pH	696:697	arg1	range					699:703	the pH range	692:703	the pH range from 8 to 11	692:716	In the pH range from 8 to 11, micron thick and optically transparent LbL films are obtained by automated dipping when dried regularly in a stream of air.
25590696	9	15	from	changes	1575:1581	arg1	mobility					1601:1608	the polycation mobility	1586:1608	the polycation mobility	1586:1608	We attribute the switchability of the mechanical properties and the propensity for self-repair to changes in the polycation mobility that are brought about by the plastifying effect of water.
25590696	3	16	theme	pH	560:561	arg1	values					563:568	higher pH values	553:568	higher pH values	553:568	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	1	17	dep	properties	160:169	arg1	properties					160:169	multiple interesting properties	139:169	multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability)	139:257	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	17	dep	properties	160:169	arg1	self-healing					215:226	self-healing	215:226	self-healing	215:226	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	17	dep	properties	160:169	arg1	strength					183:190	mechanical strength	172:190	mechanical strength	172:190	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	17	dep	properties	160:169	arg1	transparency					201:212	optical transparency	193:212	optical transparency	193:212	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	17	dep	properties	160:169	arg1	biodegradability					241:256	partial biodegradability	233:256	partial biodegradability	233:256	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	8	18	theme	stable	1459:1464	arg1	junctions					1466:1474	mechanically stable junctions	1446:1474	mechanically stable junctions	1446:1474	Wet film surfaces even amalgamate upon contact to yield mechanically stable junctions.
25590696	3	19	theme	chemical	505:512	arg1	composition					514:524	different chemical composition	495:524	different chemical composition	495:524	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	3	20	theme	composition	514:524	arg1	films					486:490	films	486:490	films of different chemical composition	486:524	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	2	21	used	used	277:280	arg2	We					274:275	We	274:275	We	274:275	We used Layer-by-Layer assembly to prepare micron thick wood-inspired films from anionic nanofibrillated cellulose and cationic poly(vinyl amine).
25590696	1	22	theme	optical	193:199	arg1	properties					160:169	multiple interesting properties	139:169	multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability)	139:257	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	22	theme	optical	193:199	arg1	transparency					201:212	optical transparency	193:212	optical transparency	193:212	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	6	23	theme	properties	1206:1215	arg1	switching					1176:1184	reversible switching	1165:1184	reversible switching of their mechanical properties	1165:1215	Experiments at different humidities revealed the plasticizing effect of water in the films and allowed reversible switching of their mechanical properties.
25590696	5	24	theme	Young	967:971	arg1	moduli					975:980	Young's moduli	967:980	Young's moduli up to about 18 GPa	967:999	Films with a size of 10 cm(2) or more can be peeled from flat surfaces; they show tensile strengths up to about 250 MPa and Young's moduli up to about 18 GPa as controlled by the polycation/polyanion ratio of the film.
25590696	6	25	theme	plasticizing	1111:1122	arg1	effect					1124:1129	the plasticizing effect	1107:1129	the plasticizing effect of water in the films	1107:1151	Experiments at different humidities revealed the plasticizing effect of water in the films and allowed reversible switching of their mechanical properties.
25590696	3	26	theme	film	425:428	arg1	growth					430:435	The film growth	421:435	The film growth	421:435	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	7	27	theme	Young	1260:1264	arg1	modulus					1268:1274	Young's modulus	1260:1274	Young's modulus: 16 GPa, strain at break: 1.7%	1260:1305	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	0	28	theme	multiproperty	13:25	arg1	materials					27:35	Bio-inspired multiproperty materials	0:35	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.	0:106	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.
25590696	5	29	dep	GPa	997:999	arg1	up					982:983	up	982:983	up	982:983	Films with a size of 10 cm(2) or more can be peeled from flat surfaces; they show tensile strengths up to about 250 MPa and Young's moduli up to about 18 GPa as controlled by the polycation/polyanion ratio of the film.
25590696	2	30	theme	anionic	355:361	arg1	cellulose					379:387	anionic nanofibrillated cellulose	355:387	anionic nanofibrillated cellulose	355:387	We used Layer-by-Layer assembly to prepare micron thick wood-inspired films from anionic nanofibrillated cellulose and cationic poly(vinyl amine).
25590696	4	31	dep	11	715:716	arg1	to					712:713	to	712:713	to	712:713	In the pH range from 8 to 11, micron thick and optically transparent LbL films are obtained by automated dipping when dried regularly in a stream of air.
25590696	0	32	theme	Bio-inspired	0:11	arg1	materials					27:35	Bio-inspired multiproperty materials	0:35	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.	0:106	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.
25590696	3	33	dep	55	666:667	arg1	to					663:664	to	663:664	to	663:664	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	4	34	theme	automated	784:792	arg1	dipping					794:800	automated dipping	784:800	automated dipping	784:800	In the pH range from 8 to 11, micron thick and optically transparent LbL films are obtained by automated dipping when dried regularly in a stream of air.
25590696	3	35	theme	different	456:464	arg1	values					469:474	different pH values	456:474	different pH values	456:474	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	6	36	theme	mechanical	1195:1204	arg1	properties					1206:1215	their mechanical properties	1189:1215	their mechanical properties	1189:1215	Experiments at different humidities revealed the plasticizing effect of water in the films and allowed reversible switching of their mechanical properties.
25590696	3	37	theme	polycation	586:595	arg1	content					597:603	a higher polycation content	577:603	a higher polycation content	577:603	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	1	38	theme	Nanocomposite	108:120	arg1	films					122:126	Nanocomposite films	108:126	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability)	108:257	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	7	39	from	break	1295:1299	arg1	strain					1285:1290	strain	1285:1290	strain	1285:1290	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	7	39	from	break	1295:1299	arg1	GPa					1280:1282	16 GPa	1277:1282	16 GPa	1277:1282	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	5	40	theme	polycation/polyanion	1022:1041	arg1	ratio					1043:1047	the polycation/polyanion ratio	1018:1047	the polycation/polyanion ratio of the film	1018:1059	Films with a size of 10 cm(2) or more can be peeled from flat surfaces; they show tensile strengths up to about 250 MPa and Young's moduli up to about 18 GPa as controlled by the polycation/polyanion ratio of the film.
25590696	7	41	dep	modulus	1349:1355	arg1	GPa					1362:1364	0.1 GPa	1358:1364	0.1 GPa	1358:1364	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	7	41	dep	modulus	1349:1355	arg1	%					1386:1386	49%	1384:1386	49%	1384:1386	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	7	41	dep	modulus	1349:1355	arg1	strain					1367:1372	strain	1367:1372	strain at break	1367:1381	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	3	42	theme	growth	637:642	arg1	increments					644:653	6 times higher film growth increments	617:653	6 times higher film growth increments (from 9 to 55 nm per layer pair)	617:686	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	2	43	theme	wood-inspired	330:342	arg1	films					344:348	micron thick wood-inspired films	317:348	micron thick wood-inspired films	317:348	We used Layer-by-Layer assembly to prepare micron thick wood-inspired films from anionic nanofibrillated cellulose and cationic poly(vinyl amine).
25590696	7	44	theme	dry	1226:1228	arg1	films					1230:1234	dry films	1226:1234	dry films	1226:1234	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	6	45	theme	different	1077:1085	arg1	humidities					1087:1096	different humidities	1077:1096	different humidities	1077:1096	Experiments at different humidities revealed the plasticizing effect of water in the films and allowed reversible switching of their mechanical properties.
25590696	4	46	theme	LbL	758:760	arg1	films					762:766	micron thick and optically transparent LbL films	719:766	micron thick and optically transparent LbL films	719:766	In the pH range from 8 to 11, micron thick and optically transparent LbL films are obtained by automated dipping when dried regularly in a stream of air.
25590696	1	47	theme	partial	233:239	arg1	properties					160:169	multiple interesting properties	139:169	multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability)	139:257	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	47	theme	partial	233:239	arg1	biodegradability					241:256	partial biodegradability	233:256	partial biodegradability	233:256	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	0	48	theme	strong	38:43	arg1	nanostructures					92:105	strong, self-healing, and transparent artificial wood nanostructures	38:105	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.	0:106	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.
25590696	9	49	theme	polycation	1590:1599	arg1	mobility					1601:1608	the polycation mobility	1586:1608	the polycation mobility	1586:1608	We attribute the switchability of the mechanical properties and the propensity for self-repair to changes in the polycation mobility that are brought about by the plastifying effect of water.
25590696	3	50	theme	higher	625:630	arg1	increments					644:653	6 times higher film growth increments	617:653	6 times higher film growth increments (from 9 to 55 nm per layer pair)	617:686	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	4	51	theme	transparent	746:756	arg1	films					762:766	micron thick and optically transparent LbL films	719:766	micron thick and optically transparent LbL films	719:766	In the pH range from 8 to 11, micron thick and optically transparent LbL films are obtained by automated dipping when dried regularly in a stream of air.
25590696	6	52	theme	reversible	1165:1174	arg1	switching					1176:1184	reversible switching	1165:1184	reversible switching of their mechanical properties	1165:1215	Experiments at different humidities revealed the plasticizing effect of water in the films and allowed reversible switching of their mechanical properties.
25590696	3	53	theme	film	632:635	arg1	increments					644:653	6 times higher film growth increments	617:653	6 times higher film growth increments (from 9 to 55 nm per layer pair)	617:686	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	3	54	theme	higher	579:584	arg1	content					597:603	a higher polycation content	577:603	a higher polycation content	577:603	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	2	55	theme	cationic	393:400	arg1	poly					402:405	cationic poly	393:405	cationic poly(vinyl amine)	393:418	We used Layer-by-Layer assembly to prepare micron thick wood-inspired films from anionic nanofibrillated cellulose and cationic poly(vinyl amine).
25590696	2	55	theme	cationic	393:400	arg1	amine					413:417	vinyl amine	407:417	vinyl amine	407:417	We used Layer-by-Layer assembly to prepare micron thick wood-inspired films from anionic nanofibrillated cellulose and cationic poly(vinyl amine).
25590696	0	56	theme	self-healing	46:57	arg1	nanostructures					92:105	strong, self-healing, and transparent artificial wood nanostructures	38:105	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.	0:106	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.
25590696	5	57	dep	MPa	959:961	arg1	up					943:944	up	943:944	up	943:944	Films with a size of 10 cm(2) or more can be peeled from flat surfaces; they show tensile strengths up to about 250 MPa and Young's moduli up to about 18 GPa as controlled by the polycation/polyanion ratio of the film.
25590696	5	58	with	Films	843:847	arg1	size					856:859	a size	854:859	a size of 10 cm(2) or more	854:879	Films with a size of 10 cm(2) or more can be peeled from flat surfaces; they show tensile strengths up to about 250 MPa and Young's moduli up to about 18 GPa as controlled by the polycation/polyanion ratio of the film.
25590696	1	59	theme	mechanical	172:181	arg1	properties					160:169	multiple interesting properties	139:169	multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability)	139:257	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	59	theme	mechanical	172:181	arg1	strength					183:190	mechanical strength	172:190	mechanical strength	172:190	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	7	60	dep	modulus	1268:1274	arg1	%					1305:1305	1.7%	1302:1305	Young's modulus: 16 GPa, strain at break: 1.7%	1260:1305	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	7	60	dep	modulus	1268:1274	arg1	strain					1285:1290	strain	1285:1290	strain	1285:1290	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	7	60	dep	modulus	1268:1274	arg1	GPa					1280:1282	16 GPa	1277:1282	16 GPa	1277:1282	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	1	61	theme	multiple	139:146	arg1	self-healing					215:226	self-healing	215:226	self-healing	215:226	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	61	theme	multiple	139:146	arg1	properties					160:169	multiple interesting properties	139:169	multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability)	139:257	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	61	theme	multiple	139:146	arg1	strength					183:190	mechanical strength	172:190	mechanical strength	172:190	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	61	theme	multiple	139:146	arg1	transparency					201:212	optical transparency	193:212	optical transparency	193:212	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	61	theme	multiple	139:146	arg1	biodegradability					241:256	partial biodegradability	233:256	partial biodegradability	233:256	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	3	62	theme	pH	466:467	arg1	values					469:474	different pH values	456:474	different pH values	456:474	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	4	63	theme	air	838:840	arg1	stream					828:833	a stream	826:833	a stream of air	826:840	In the pH range from 8 to 11, micron thick and optically transparent LbL films are obtained by automated dipping when dried regularly in a stream of air.
25590696	5	64	theme	more	876:879	arg1	size					856:859	a size	854:859	a size of 10 cm(2) or more	854:879	Films with a size of 10 cm(2) or more can be peeled from flat surfaces; they show tensile strengths up to about 250 MPa and Young's moduli up to about 18 GPa as controlled by the polycation/polyanion ratio of the film.
25590696	4	65	theme	thick	726:730	arg1	films					762:766	micron thick and optically transparent LbL films	719:766	micron thick and optically transparent LbL films	719:766	In the pH range from 8 to 11, micron thick and optically transparent LbL films are obtained by automated dipping when dried regularly in a stream of air.
25590696	5	66	theme	flat	900:903	arg1	surfaces					905:912	flat surfaces	900:912	flat surfaces	900:912	Films with a size of 10 cm(2) or more can be peeled from flat surfaces; they show tensile strengths up to about 250 MPa and Young's moduli up to about 18 GPa as controlled by the polycation/polyanion ratio of the film.
25590696	1	67	theme	interesting	148:158	arg1	self-healing					215:226	self-healing	215:226	self-healing	215:226	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	67	theme	interesting	148:158	arg1	properties					160:169	multiple interesting properties	139:169	multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability)	139:257	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	67	theme	interesting	148:158	arg1	strength					183:190	mechanical strength	172:190	mechanical strength	172:190	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	67	theme	interesting	148:158	arg1	transparency					201:212	optical transparency	193:212	optical transparency	193:212	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	1	67	theme	interesting	148:158	arg1	biodegradability					241:256	partial biodegradability	233:256	partial biodegradability	233:256	Nanocomposite films possessing multiple interesting properties (mechanical strength, optical transparency, self-healing, and partial biodegradability) are discussed.
25590696	8	68	theme	film	1394:1397	arg1	surfaces					1399:1406	Wet film surfaces	1390:1406	Wet film surfaces	1390:1406	Wet film surfaces even amalgamate upon contact to yield mechanically stable junctions.
25590696	7	69	dep	soft	1323:1326	arg1	modulus					1349:1355	Young's modulus	1341:1355	Young's modulus	1341:1355	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	7	69	dep	soft	1323:1326	arg1	films					1313:1317	wet films	1309:1317	wet films	1309:1317	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	5	70	theme	tensile	925:931	arg1	strengths					933:941	tensile strengths	925:941	tensile strengths up to about 250 MPa	925:961	Films with a size of 10 cm(2) or more can be peeled from flat surfaces; they show tensile strengths up to about 250 MPa and Young's moduli up to about 18 GPa as controlled by the polycation/polyanion ratio of the film.
25590696	0	71	dep	materials	27:35	arg1	nanostructures					92:105	strong, self-healing, and transparent artificial wood nanostructures	38:105	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.	0:106	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.
25590696	7	72	theme	Young	1341:1345	arg1	modulus					1349:1355	Young's modulus	1341:1355	Young's modulus	1341:1355	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	7	72	theme	Young	1341:1345	arg1	films					1313:1317	wet films	1309:1317	wet films	1309:1317	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	2	73	theme	nanofibrillated	363:377	arg1	cellulose					379:387	anionic nanofibrillated cellulose	355:387	anionic nanofibrillated cellulose	355:387	We used Layer-by-Layer assembly to prepare micron thick wood-inspired films from anionic nanofibrillated cellulose and cationic poly(vinyl amine).
25590696	6	74	from	humidities	1087:1096	arg1	Experiments					1062:1072	Experiments	1062:1072	Experiments at different humidities	1062:1096	Experiments at different humidities revealed the plasticizing effect of water in the films and allowed reversible switching of their mechanical properties.
25590696	3	75	theme	different	495:503	arg1	composition					514:524	different chemical composition	495:524	different chemical composition	495:524	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	0	76	theme	transparent	64:74	arg1	nanostructures					92:105	strong, self-healing, and transparent artificial wood nanostructures	38:105	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.	0:106	Bio-inspired multiproperty materials: strong, self-healing, and transparent artificial wood nanostructures.
25590696	7	77	theme	wet	1309:1311	arg1	films					1313:1317	wet films	1309:1317	wet films	1309:1317	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	7	77	theme	wet	1309:1311	arg1	modulus					1349:1355	Young's modulus	1341:1355	Young's modulus	1341:1355	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	7	78	dep	brittle	1251:1257	arg1	modulus					1268:1274	Young's modulus	1260:1274	Young's modulus: 16 GPa, strain at break: 1.7%	1260:1305	Whereas dry films are strong and brittle (Young's modulus: 16 GPa, strain at break: 1.7%), wet films are soft and ductile (Young's modulus: 0.1 GPa, strain at break: 49%).
25590696	6	79	from	effect	1124:1129	arg1	films					1147:1151	the films	1143:1151	the films	1143:1151	Experiments at different humidities revealed the plasticizing effect of water in the films and allowed reversible switching of their mechanical properties.
25590696	3	80	theme	higher	553:558	arg1	values					563:568	higher pH values	553:568	higher pH values	553:568	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	3	81	dep	increments	644:653	arg1	nm					669:670	from 9 to 55 nm	656:670	from 9 to 55 nm per layer pair	656:685	The film growth was carried out at different pH values to obtain films of different chemical composition, whereby, and as expected, higher pH values led to a higher polycation content and also to 6 times higher film growth increments (from 9 to 55 nm per layer pair).
25590696	2	82	theme	vinyl	407:411	arg1	poly					402:405	cationic poly	393:405	cationic poly(vinyl amine)	393:418	We used Layer-by-Layer assembly to prepare micron thick wood-inspired films from anionic nanofibrillated cellulose and cationic poly(vinyl amine).
25590696	2	82	theme	vinyl	407:411	arg1	amine					413:417	vinyl amine	407:417	vinyl amine	407:417	We used Layer-by-Layer assembly to prepare micron thick wood-inspired films from anionic nanofibrillated cellulose and cationic poly(vinyl amine).
25590696	8	83	theme	Wet	1390:1392	arg1	surfaces					1399:1406	Wet film surfaces	1390:1406	Wet film surfaces	1390:1406	Wet film surfaces even amalgamate upon contact to yield mechanically stable junctions.
25590696	2	84	theme	Layer-by-Layer	282:295	arg1	assembly					297:304	Layer-by-Layer assembly	282:304	Layer-by-Layer assembly	282:304	We used Layer-by-Layer assembly to prepare micron thick wood-inspired films from anionic nanofibrillated cellulose and cationic poly(vinyl amine).
28976638	0	0	theme	starch	75:80	arg1	grains					82:87	maize starch grains	69:87	maize starch grains	69:87	Bivariate flow cytometric analysis and sorting of different types of maize starch grains.
28976638	2	1	theme	natural	381:387	arg1	particles					400:408	separating natural biological particles	370:408	separating natural biological particles	370:408	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	5	2	theme	high-amylose	1260:1271	arg1	starch					1279:1284	high-amylose maize starch	1260:1284	high-amylose maize starch	1260:1284	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	5	3	theme	pop	1209:1211	arg1	maize					1213:1217	normal maize, sweet maize, and pop maize	1178:1217	normal maize, sweet maize, and pop maize	1178:1217	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	0	4	theme	maize	69:73	arg1	grains					82:87	maize starch grains	69:87	maize starch grains	69:87	Bivariate flow cytometric analysis and sorting of different types of maize starch grains.
28976638	2	5	theme	separating	370:379	arg1	particles					400:408	separating natural biological particles	370:408	separating natural biological particles	370:408	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	4	6	theme	long	1081:1084	arg1	time					1086:1089	a relatively long time	1068:1089	a relatively long time	1068:1089	The results showed that a 0.25% Gelzan solution served as an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time.
28976638	3	7	theme	flow	836:839	arg1	sorting					841:847	flow sorting	836:847	flow sorting to observe their morphology and particle-size distribution	836:906	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	3	8	theme	high-amylose	694:705	arg1	maize					707:711	high-amylose maize	694:711	high-amylose maize	694:711	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	3	9	attach	isolated	655:662	arg1	maize					718:722	pop maize	714:722	pop maize	714:722	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	3	9	attach	isolated	655:662	arg1	maize					707:711	high-amylose maize	694:711	high-amylose maize	694:711	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	3	9	attach	isolated	655:662	arg1	maize					736:740	normal maize	729:740	normal maize	729:740	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	3	9	attach	isolated	655:662	arg1	maize					674:678	waxy maize	669:678	waxy maize	669:678	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	3	9	attach	isolated	655:662	arg2	starches					646:653	starches	646:653	starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize	646:740	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	3	9	attach	isolated	655:662	arg1	maize					687:691	sweet maize	681:691	sweet maize	681:691	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	0	10	theme	grains	82:87	arg1	types					60:64	different types	50:64	different types of maize starch grains	50:87	Bivariate flow cytometric analysis and sorting of different types of maize starch grains.
28976638	7	11	theme	granule	1464:1470	arg1	size					1472:1475	granule size	1464:1475	granule size	1464:1475	The subgroups sorted by flow cytometer were determined and verified in terms of morphology and granule size by scanning electron microscopy and laser particle distribution analyzer.
28976638	8	12	theme	starch	1648:1653	arg1	granules					1655:1662	sorting starch granules	1640:1662	sorting starch granules	1640:1662	Results showed that flow cytometry can be regarded as a novel method for classifying and sorting starch granules.
28976638	5	13	theme	cytometric	1111:1120	arg1	distributions					1133:1145	The bivariate flow cytometric population distributions	1092:1145	The bivariate flow cytometric population distributions	1092:1145	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	2	14	theme	light	538:542	arg1	beam					544:547	a light beam	536:547	a light beam	536:547	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	3	15	theme	pop	714:716	arg1	maize					718:722	pop maize	714:722	pop maize	714:722	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	4	16	theme	individual	998:1007	arg1	particles					1016:1024	individual starch particles	998:1024	individual starch particles homogeneously dispersed in suspension for a relatively long time	998:1089	The results showed that a 0.25% Gelzan solution served as an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time.
28976638	2	17	theme	flow	286:289	arg1	sorting					291:297	flow sorting	286:297	flow sorting	286:297	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	4	18	theme	%	939:939	arg1	reagent					978:984	an optimal reagent	967:984	an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time	967:1089	The results showed that a 0.25% Gelzan solution served as an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time.
28976638	4	18	theme	%	939:939	arg1	solution					948:955	a 0.25% Gelzan solution	933:955	a 0.25% Gelzan solution	933:955	The results showed that a 0.25% Gelzan solution served as an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time.
28976638	1	19	theme	physicochemical	179:193	arg1	properties					195:204	the physicochemical properties	175:204	the physicochemical properties	175:204	Particle-size distribution, granular structure, and composition significantly affect the physicochemical properties, rheological properties, and nutritional function of starch.
28976638	0	20	theme	flow	10:13	arg1	analysis					26:33	Bivariate flow cytometric analysis	0:33	Bivariate flow cytometric analysis	0:33	Bivariate flow cytometric analysis and sorting of different types of maize starch grains.
28976638	8	21	theme	flow	1571:1574	arg1	cytometry					1576:1584	flow cytometry	1571:1584	flow cytometry	1571:1584	Results showed that flow cytometry can be regarded as a novel method for classifying and sorting starch granules.
28976638	8	21	theme	flow	1571:1574	arg1	method					1613:1618	a novel method	1605:1618	a novel method for classifying and sorting starch granules	1605:1662	Results showed that flow cytometry can be regarded as a novel method for classifying and sorting starch granules.
28976638	2	22	theme	efficient	336:344	arg1	ways					346:349	convenient and efficient ways	321:349	convenient and efficient ways	321:349	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	7	23	theme	size	1472:1475	arg1	terms					1440:1444	terms	1440:1444	terms of morphology and granule size	1440:1475	The subgroups sorted by flow cytometer were determined and verified in terms of morphology and granule size by scanning electron microscopy and laser particle distribution analyzer.
28976638	2	24	theme	other	413:417	arg1	substances					419:428	other substances	413:428	other substances	413:428	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	0	25	theme	Bivariate	0:8	arg1	analysis					26:33	Bivariate flow cytometric analysis	0:33	Bivariate flow cytometric analysis	0:33	Bivariate flow cytometric analysis and sorting of different types of maize starch grains.
28976638	5	26	theme	sweet	1192:1196	arg1	maize					1198:1202	sweet maize	1192:1202	sweet maize	1192:1202	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	7	27	theme	particle	1519:1526	arg1	analyzer					1541:1548	laser particle distribution analyzer	1513:1548	laser particle distribution analyzer	1513:1548	The subgroups sorted by flow cytometer were determined and verified in terms of morphology and granule size by scanning electron microscopy and laser particle distribution analyzer.
28976638	7	28	theme	morphology	1449:1458	arg1	terms					1440:1444	terms	1440:1444	terms of morphology and granule size	1440:1475	The subgroups sorted by flow cytometer were determined and verified in terms of morphology and granule size by scanning electron microscopy and laser particle distribution analyzer.
28976638	5	29	theme	maize	1198:1202	arg1	maize					1213:1217	normal maize, sweet maize, and pop maize	1178:1217	normal maize, sweet maize, and pop maize	1178:1217	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	9	30	theme	Cytometry	1713:1721	arg1	Advancement					1698:1708	Advancement	1698:1708	Advancement of Cytometry	1698:1721	© 2017 International Society for Advancement of Cytometry.
28976638	2	31	theme	convenient	321:330	arg1	ways					346:349	convenient and efficient ways	321:349	convenient and efficient ways	321:349	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	1	32	theme	rheological	207:217	arg1	properties					219:228	rheological properties	207:228	rheological properties	207:228	Particle-size distribution, granular structure, and composition significantly affect the physicochemical properties, rheological properties, and nutritional function of starch.
28976638	0	33	theme	cytometric	15:24	arg1	analysis					26:33	Bivariate flow cytometric analysis	0:33	Bivariate flow cytometric analysis	0:33	Bivariate flow cytometric analysis and sorting of different types of maize starch grains.
28976638	3	34	theme	normal	729:734	arg1	maize					736:740	normal maize	729:740	normal maize	729:740	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	5	35	theme	maize	1273:1277	arg1	starch					1279:1284	high-amylose maize starch	1260:1284	high-amylose maize starch	1260:1284	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	2	36	theme	component	508:516	arg1	response					491:498	the differential response	474:498	the differential response of each component to stimulation by a light beam	474:547	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	2	37	theme	differential	478:489	arg1	response					491:498	the differential response	474:498	the differential response of each component to stimulation by a light beam	474:547	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	4	38	theme	Gelzan	941:946	arg1	reagent					978:984	an optimal reagent	967:984	an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time	967:1089	The results showed that a 0.25% Gelzan solution served as an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time.
28976638	4	38	theme	Gelzan	941:946	arg1	solution					948:955	a 0.25% Gelzan solution	933:955	a 0.25% Gelzan solution	933:955	The results showed that a 0.25% Gelzan solution served as an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time.
28976638	3	39	theme	waxy	669:672	arg1	maize					674:678	waxy maize	669:678	waxy maize	669:678	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	8	40	theme	sorting	1640:1646	arg1	granules					1655:1662	sorting starch granules	1640:1662	sorting starch granules	1640:1662	Results showed that flow cytometry can be regarded as a novel method for classifying and sorting starch granules.
28976638	5	41	theme	maize	1213:1217	arg1	starches					1166:1173	the starches	1162:1173	the starches of normal maize, sweet maize, and pop maize	1162:1217	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	3	42	theme	various	773:779	arg1	subgroups					781:789	various subgroups	773:789	various subgroups	773:789	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	4	43	theme	0.25	935:938	arg1	%					939:939	%	939:939	%	939:939	The results showed that a 0.25% Gelzan solution served as an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time.
28976638	3	44	theme	starches	646:653	arg1	types					637:641	different types	627:641	different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize	627:740	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	7	45	theme	flow	1393:1396	arg1	cytometer					1398:1406	flow cytometer	1393:1406	flow cytometer	1393:1406	The subgroups sorted by flow cytometer were determined and verified in terms of morphology and granule size by scanning electron microscopy and laser particle distribution analyzer.
28976638	0	46	theme	different	50:58	arg1	types					60:64	different types	50:64	different types of maize starch grains	50:87	Bivariate flow cytometric analysis and sorting of different types of maize starch grains.
28976638	5	47	theme	normal	1178:1183	arg1	maize					1213:1217	normal maize, sweet maize, and pop maize	1178:1217	normal maize, sweet maize, and pop maize	1178:1217	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	2	48	theme	parameters	600:609	arg1	analysis					588:595	the correlation analysis	572:595	the correlation analysis of parameters	572:609	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	5	49	theme	bivariate	1096:1104	arg1	distributions					1133:1145	The bivariate flow cytometric population distributions	1092:1145	The bivariate flow cytometric population distributions	1092:1145	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	5	50	theme	population	1122:1131	arg1	distributions					1133:1145	The bivariate flow cytometric population distributions	1092:1145	The bivariate flow cytometric population distributions	1092:1145	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	4	51	theme	starch	1009:1014	arg1	particles					1016:1024	individual starch particles	998:1024	individual starch particles homogeneously dispersed in suspension for a relatively long time	998:1089	The results showed that a 0.25% Gelzan solution served as an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time.
28976638	5	52	theme	maize	1185:1189	arg1	maize					1213:1217	normal maize, sweet maize, and pop maize	1178:1217	normal maize, sweet maize, and pop maize	1178:1217	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	6	53	theme	maize	1314:1318	arg1	starch					1320:1325	Waxy maize starch	1309:1325	Waxy maize starch	1309:1325	Waxy maize starch, conversely, showed three subpopulations.
28976638	3	54	theme	sweet	681:685	arg1	maize					687:691	sweet maize	681:691	sweet maize	681:691	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	5	55	contain	had	1286:1288	arg2	subgroup					1299:1306	only one subgroup	1290:1306	only one subgroup	1290:1306	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	5	55	contain	had	1286:1288	arg1	starch					1279:1284	high-amylose maize starch	1260:1284	high-amylose maize starch	1260:1284	The bivariate flow cytometric population distributions indicated that the starches of normal maize, sweet maize, and pop maize were divided into two subgroups, whereas high-amylose maize starch had only one subgroup.
28976638	1	56	theme	granular	118:125	arg1	structure					127:135	granular structure	118:135	granular structure	118:135	Particle-size distribution, granular structure, and composition significantly affect the physicochemical properties, rheological properties, and nutritional function of starch.
28976638	3	57	theme	different	627:635	arg1	types					637:641	different types	627:641	different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize	627:740	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	6	58	theme	Waxy	1309:1312	arg1	starch					1320:1325	Waxy maize starch	1309:1325	Waxy maize starch	1309:1325	Waxy maize starch, conversely, showed three subpopulations.
28976638	7	59	theme	distribution	1528:1539	arg1	analyzer					1541:1548	laser particle distribution analyzer	1513:1548	laser particle distribution analyzer	1513:1548	The subgroups sorted by flow cytometer were determined and verified in terms of morphology and granule size by scanning electron microscopy and laser particle distribution analyzer.
28976638	7	60	theme	scanning	1480:1487	arg1	microscopy					1498:1507	scanning electron microscopy	1480:1507	scanning electron microscopy	1480:1507	The subgroups sorted by flow cytometer were determined and verified in terms of morphology and granule size by scanning electron microscopy and laser particle distribution analyzer.
28976638	1	61	theme	Particle-size	90:102	arg1	distribution					104:115	Particle-size distribution	90:115	Particle-size distribution	90:115	Particle-size distribution, granular structure, and composition significantly affect the physicochemical properties, rheological properties, and nutritional function of starch.
28976638	1	62	theme	nutritional	235:245	arg1	function					247:254	nutritional function	235:254	nutritional function	235:254	Particle-size distribution, granular structure, and composition significantly affect the physicochemical properties, rheological properties, and nutritional function of starch.
28976638	2	63	theme	correlation	576:586	arg1	analysis					588:595	the correlation analysis	572:595	the correlation analysis of parameters	572:609	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	8	64	theme	novel	1607:1611	arg1	cytometry					1576:1584	flow cytometry	1571:1584	flow cytometry	1571:1584	Results showed that flow cytometry can be regarded as a novel method for classifying and sorting starch granules.
28976638	8	64	theme	novel	1607:1611	arg1	method					1613:1618	a novel method	1605:1618	a novel method for classifying and sorting starch granules	1605:1662	Results showed that flow cytometry can be regarded as a novel method for classifying and sorting starch granules.
28976638	4	65	theme	optimal	970:976	arg1	reagent					978:984	an optimal reagent	967:984	an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time	967:1089	The results showed that a 0.25% Gelzan solution served as an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time.
28976638	4	65	theme	optimal	970:976	arg1	solution					948:955	a 0.25% Gelzan solution	933:955	a 0.25% Gelzan solution	933:955	The results showed that a 0.25% Gelzan solution served as an optimal reagent for keeping individual starch particles homogeneously dispersed in suspension for a relatively long time.
28976638	7	66	theme	laser	1513:1517	arg1	analyzer					1541:1548	laser particle distribution analyzer	1513:1548	laser particle distribution analyzer	1513:1548	The subgroups sorted by flow cytometer were determined and verified in terms of morphology and granule size by scanning electron microscopy and laser particle distribution analyzer.
28976638	2	67	theme	Flow	267:270	arg1	cytometry					272:280	Flow cytometry	267:280	Flow cytometry	267:280	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	0	68	theme	types	60:64	arg1	sorting					39:45	sorting	39:45	sorting	39:45	Bivariate flow cytometric analysis and sorting of different types of maize starch grains.
28976638	0	68	theme	types	60:64	arg1	analysis					26:33	Bivariate flow cytometric analysis	0:33	Bivariate flow cytometric analysis	0:33	Bivariate flow cytometric analysis and sorting of different types of maize starch grains.
28976638	7	69	theme	electron	1489:1496	arg1	microscopy					1498:1507	scanning electron microscopy	1480:1507	scanning electron microscopy	1480:1507	The subgroups sorted by flow cytometer were determined and verified in terms of morphology and granule size by scanning electron microscopy and laser particle distribution analyzer.
28976638	3	70	theme	flow	794:797	arg1	cytometer					799:807	flow cytometer	794:807	flow cytometer	794:807	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	2	71	theme	biological	389:398	arg1	particles					400:408	separating natural biological particles	370:408	separating natural biological particles	370:408	Flow cytometry and flow sorting are widely considered convenient and efficient ways of classifying and separating natural biological particles or other substances into subpopulations, respectively, based on the differential response of each component to stimulation by a light beam; the results allow for the correlation analysis of parameters.
28976638	3	72	theme	particle-size	881:893	arg1	distribution					895:906	particle-size distribution	881:906	particle-size distribution	881:906	In this study, different types of starches isolated from waxy maize, sweet maize, high-amylose maize, pop maize, and normal maize were initially classified into various subgroups by flow cytometer and then collected through flow sorting to observe their morphology and particle-size distribution.
28976638	1	73	theme	starch	259:264	arg1	properties					195:204	the physicochemical properties	175:204	the physicochemical properties	175:204	Particle-size distribution, granular structure, and composition significantly affect the physicochemical properties, rheological properties, and nutritional function of starch.
28976638	1	73	theme	starch	259:264	arg1	properties					219:228	rheological properties	207:228	rheological properties	207:228	Particle-size distribution, granular structure, and composition significantly affect the physicochemical properties, rheological properties, and nutritional function of starch.
28976638	1	73	theme	starch	259:264	arg1	function					247:254	nutritional function	235:254	nutritional function	235:254	Particle-size distribution, granular structure, and composition significantly affect the physicochemical properties, rheological properties, and nutritional function of starch.
28322959	0	0	theme	alginate	93:100	arg1	beads					102:106	cross-linked alginate beads	80:106	cross-linked alginate beads	80:106	Synergistic effect of divalent cations in improving technological properties of cross-linked alginate beads.
28322959	5	1	theme	aqueous	740:746	arg1	solutions					753:761	the aqueous feed solutions	736:761	the aqueous feed solutions (sodium alginate and prednisolone concentration)	736:810	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	1	theme	aqueous	740:746	arg1	alginate					771:778	sodium alginate	764:778	sodium alginate	764:778	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	1	theme	aqueous	740:746	arg1	concentration					797:809	prednisolone concentration	784:809	prednisolone concentration	784:809	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	1	2	theme	important	158:166	arg1	variables					168:176	the most important variables	149:176	the most important variables in ionotropic gelation	149:199	Gelling solution parameters are some of the most important variables in ionotropic gelation and consequently influence the technological characteristics of the product.
28322959	4	3	theme	Alginate	559:566	arg1	beads					568:572	Alginate beads	559:572	Alginate beads containing prednisolone (P) as model drug	559:614	Alginate beads containing prednisolone (P) as model drug were prepared by prilling technique.
28322959	0	4	theme	cross-linked	80:91	arg1	beads					102:106	cross-linked alginate beads	80:106	cross-linked alginate beads	80:106	Synergistic effect of divalent cations in improving technological properties of cross-linked alginate beads.
28322959	2	5	theme	cations	355:361	arg1	use					339:341	the simultaneous use	322:341	the simultaneous use of multiple cations as gelling agents	322:379	To date, only a few studies have focused on the simultaneous use of multiple cations as gelling agents.
28322959	5	6	theme	gelation	699:706	arg1	process					708:714	the ionotropic gelation process	684:714	the ionotropic gelation process	684:714	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	1	7	from	variables	168:176	arg1	gelation					192:199	ionotropic gelation	181:199	ionotropic gelation	181:199	Gelling solution parameters are some of the most important variables in ionotropic gelation and consequently influence the technological characteristics of the product.
28322959	8	8	theme	alginate	1360:1367	arg1	alginate					1360:1367	alginate	1360:1367	alginate	1360:1367	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	8	8	theme	alginate	1360:1367	arg1	groups					1350:1355	guluronic groups	1340:1355	guluronic groups of alginate	1340:1367	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	0	9	theme	beads	102:106	arg1	properties					66:75	technological properties	52:75	technological properties of cross-linked alginate beads	52:106	Synergistic effect of divalent cations in improving technological properties of cross-linked alginate beads.
28322959	3	10	theme	alginate	518:525	arg1	beads					527:531	alginate beads formation and properties	518:556	alginate beads formation and properties	518:556	With the aim to deeply explore this possibility, in this research we investigated the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties.
28322959	3	11	theme	cations	491:497	arg1	effect					468:473	the effect	464:473	the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties	464:556	With the aim to deeply explore this possibility, in this research we investigated the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties.
28322959	7	12	theme	polymeric	1179:1187	arg1	matrix					1189:1194	the alginate polymeric matrix	1166:1194	the alginate polymeric matrix	1166:1194	Results evidenced a synergistic effect of the two cations, affecting positively both the encapsulation efficiency and the ability of the alginate polymeric matrix to control the drug release.
28322959	7	13	theme	cations	1083:1089	arg1	effect					1065:1070	a synergistic effect	1051:1070	a synergistic effect of the two cations	1051:1089	Results evidenced a synergistic effect of the two cations, affecting positively both the encapsulation efficiency and the ability of the alginate polymeric matrix to control the drug release.
28322959	8	14	theme	4:1	1246:1248	arg1	ratio					1237:1241	A Ca2+/Zn2+ ratio	1225:1241	A Ca2+/Zn2+ ratio of 4:1	1225:1248	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	8	15	theme	electrostatic	1308:1320	arg1	interactions					1322:1333	quicker electrostatic interactions	1300:1333	quicker electrostatic interactions with guluronic groups of alginate	1300:1367	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	8	16	theme	Ca2+/Zn2+	1227:1235	arg1	ratio					1237:1241	A Ca2+/Zn2+ ratio	1225:1241	A Ca2+/Zn2+ ratio of 4:1	1225:1248	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	7	17	theme	matrix	1189:1194	arg1	efficiency					1136:1145	the encapsulation efficiency	1118:1145	the encapsulation efficiency	1118:1145	Results evidenced a synergistic effect of the two cations, affecting positively both the encapsulation efficiency and the ability of the alginate polymeric matrix to control the drug release.
28322959	7	17	theme	matrix	1189:1194	arg1	ability					1155:1161	the ability	1151:1161	the ability of the alginate polymeric matrix to control the drug release	1151:1222	Results evidenced a synergistic effect of the two cations, affecting positively both the encapsulation efficiency and the ability of the alginate polymeric matrix to control the drug release.
28322959	3	18	from	effect	468:473	arg1	beads					527:531	alginate beads formation and properties	518:556	alginate beads formation and properties	518:556	With the aim to deeply explore this possibility, in this research we investigated the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties.
28322959	5	19	theme	prednisolone	784:795	arg1	solutions					753:761	the aqueous feed solutions	736:761	the aqueous feed solutions (sodium alginate and prednisolone concentration)	736:810	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	19	theme	prednisolone	784:795	arg1	concentration					797:809	prednisolone concentration	784:809	prednisolone concentration	784:809	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	1	20	theme	ionotropic	181:190	arg1	gelation					192:199	ionotropic gelation	181:199	ionotropic gelation	181:199	Gelling solution parameters are some of the most important variables in ionotropic gelation and consequently influence the technological characteristics of the product.
28322959	5	21	theme	feed	748:751	arg1	solutions					753:761	the aqueous feed solutions	736:761	the aqueous feed solutions (sodium alginate and prednisolone concentration)	736:810	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	21	theme	feed	748:751	arg1	alginate					771:778	sodium alginate	764:778	sodium alginate	764:778	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	21	theme	feed	748:751	arg1	concentration					797:809	prednisolone concentration	784:809	prednisolone concentration	784:809	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	22	dep	solutions	753:761	arg1	solutions					753:761	the aqueous feed solutions	736:761	the aqueous feed solutions (sodium alginate and prednisolone concentration)	736:810	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	22	dep	solutions	753:761	arg1	alginate					771:778	sodium alginate	764:778	sodium alginate	764:778	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	22	dep	solutions	753:761	arg1	concentration					797:809	prednisolone concentration	784:809	prednisolone concentration	784:809	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	3	23	dep	cations	491:497	arg1	Zn2+					509:512	Zn2+	509:512	Zn2+	509:512	With the aim to deeply explore this possibility, in this research we investigated the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties.
28322959	3	23	dep	cations	491:497	arg1	Ca2+					500:503	Ca2+	500:503	Ca2+	500:503	With the aim to deeply explore this possibility, in this research we investigated the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties.
28322959	3	23	dep	cations	491:497	arg1	cations					491:497	two divalent cations	478:497	two divalent cations (Ca2+ and Zn2+)	478:513	With the aim to deeply explore this possibility, in this research we investigated the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties.
28322959	0	24	theme	Synergistic	0:10	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of divalent cations in improving technological properties of cross-linked alginate beads	0:106	Synergistic effect of divalent cations in improving technological properties of cross-linked alginate beads.
28322959	6	25	theme	release	939:945	arg1	performances					947:958	their in vitro release performances	924:958	their in vitro release performances	924:958	The obtained beads were characterized and their in vitro release performances were assessed in conditions simulating the gastrointestinal environment.
28322959	8	26	theme	carboxylate	1428:1438	arg1	groups					1440:1445	carboxylate groups	1428:1445	carboxylate groups of both guluronic and mannuronic moieties of alginate	1428:1499	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	8	26	theme	carboxylate	1428:1438	arg1	moieties					1480:1487	both guluronic and mannuronic moieties	1450:1487	both guluronic and mannuronic moieties of alginate	1450:1499	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	0	27	theme	divalent	22:29	arg1	cations					31:37	divalent cations	22:37	divalent cations	22:37	Synergistic effect of divalent cations in improving technological properties of cross-linked alginate beads.
28322959	8	28	with	ability	1382:1388	arg1	alginate					1360:1367	alginate	1360:1367	alginate	1360:1367	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	8	28	with	ability	1382:1388	arg1	groups					1350:1355	guluronic groups	1340:1355	guluronic groups of alginate	1340:1367	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	1	29	theme	Gelling	109:115	arg1	parameters					126:135	Gelling solution parameters	109:135	Gelling solution parameters	109:135	Gelling solution parameters are some of the most important variables in ionotropic gelation and consequently influence the technological characteristics of the product.
28322959	8	30	theme	alginate	1492:1499	arg1	moieties					1480:1487	both guluronic and mannuronic moieties	1450:1487	both guluronic and mannuronic moieties of alginate	1450:1499	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	4	31	theme	model	605:609	arg1	drug					611:614	model drug	605:614	model drug	605:614	Alginate beads containing prednisolone (P) as model drug were prepared by prilling technique.
28322959	6	32	theme	in	930:931	arg1	performances					947:958	their in vitro release performances	924:958	their in vitro release performances	924:958	The obtained beads were characterized and their in vitro release performances were assessed in conditions simulating the gastrointestinal environment.
28322959	8	33	theme	guluronic	1340:1348	arg1	alginate					1360:1367	alginate	1360:1367	alginate	1360:1367	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	8	33	theme	guluronic	1340:1348	arg1	groups					1350:1355	guluronic groups	1340:1355	guluronic groups of alginate	1340:1367	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	1	34	theme	solution	117:124	arg1	parameters					126:135	Gelling solution parameters	109:135	Gelling solution parameters	109:135	Gelling solution parameters are some of the most important variables in ionotropic gelation and consequently influence the technological characteristics of the product.
28322959	5	35	theme	cross-linking	816:828	arg1	conditions					830:839	cross-linking conditions	816:839	cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+)	816:865	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	35	theme	cross-linking	816:828	arg1	Ca2++Zn2+					856:864	Ca2++Zn2+	856:864	Ca2++Zn2+	856:864	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	35	theme	cross-linking	816:828	arg1	Zn2+					848:851	Zn2+	848:851	Zn2+	848:851	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	35	theme	cross-linking	816:828	arg1	Ca2+					842:845	Ca2+	842:845	Ca2+	842:845	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	36	theme	solutions	753:761	arg1	conditions					830:839	cross-linking conditions	816:839	cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+)	816:865	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	36	theme	solutions	753:761	arg1	Ca2++Zn2+					856:864	Ca2++Zn2+	856:864	Ca2++Zn2+	856:864	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	36	theme	solutions	753:761	arg1	composition					721:731	composition	721:731	composition of the aqueous feed solutions (sodium alginate and prednisolone concentration)	721:810	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	36	theme	solutions	753:761	arg1	Zn2+					848:851	Zn2+	848:851	Zn2+	848:851	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	36	theme	solutions	753:761	arg1	Ca2+					842:845	Ca2+	842:845	Ca2+	842:845	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	7	37	theme	alginate	1170:1177	arg1	matrix					1189:1194	the alginate polymeric matrix	1166:1194	the alginate polymeric matrix	1166:1194	Results evidenced a synergistic effect of the two cations, affecting positively both the encapsulation efficiency and the ability of the alginate polymeric matrix to control the drug release.
28322959	8	38	theme	Zn2+	1377:1380	arg1	ability					1382:1388	the Zn2+ ability	1373:1388	the Zn2+ ability	1373:1388	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	7	39	theme	encapsulation	1122:1134	arg1	efficiency					1136:1145	the encapsulation efficiency	1118:1145	the encapsulation efficiency	1118:1145	Results evidenced a synergistic effect of the two cations, affecting positively both the encapsulation efficiency and the ability of the alginate polymeric matrix to control the drug release.
28322959	0	40	theme	cations	31:37	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of divalent cations in improving technological properties of cross-linked alginate beads	0:106	Synergistic effect of divalent cations in improving technological properties of cross-linked alginate beads.
28322959	7	41	theme	drug	1211:1214	arg1	release					1216:1222	the drug release	1207:1222	the drug release	1207:1222	Results evidenced a synergistic effect of the two cations, affecting positively both the encapsulation efficiency and the ability of the alginate polymeric matrix to control the drug release.
28322959	5	42	theme	ionotropic	688:697	arg1	process					708:714	the ionotropic gelation process	684:714	the ionotropic gelation process	684:714	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	43	theme	process	708:714	arg1	variables					671:679	The main critical variables	653:679	The main critical variables	653:679	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	4	44	contain	containing	574:583	arg1	beads					568:572	Alginate beads	559:572	Alginate beads containing prednisolone (P) as model drug	559:614	Alginate beads containing prednisolone (P) as model drug were prepared by prilling technique.
28322959	4	44	contain	containing	574:583	arg2	P					599:599	P	599:599	P	599:599	Alginate beads containing prednisolone (P) as model drug were prepared by prilling technique.
28322959	4	44	contain	containing	574:583	arg2	prednisolone					585:596	prednisolone	585:596	prednisolone (P)	585:600	Alginate beads containing prednisolone (P) as model drug were prepared by prilling technique.
28322959	1	45	theme	technological	232:244	arg1	characteristics					246:260	the technological characteristics	228:260	the technological characteristics of the product	228:275	Gelling solution parameters are some of the most important variables in ionotropic gelation and consequently influence the technological characteristics of the product.
28322959	0	46	theme	technological	52:64	arg1	properties					66:75	technological properties	52:75	technological properties of cross-linked alginate beads	52:106	Synergistic effect of divalent cations in improving technological properties of cross-linked alginate beads.
28322959	0	47	link	cross-linked	80:91	arg1	beads					102:106	cross-linked alginate beads	80:106	cross-linked alginate beads	80:106	Synergistic effect of divalent cations in improving technological properties of cross-linked alginate beads.
28322959	2	48	theme	multiple	346:353	arg1	cations					355:361	multiple cations	346:361	multiple cations	346:361	To date, only a few studies have focused on the simultaneous use of multiple cations as gelling agents.
28322959	7	49	theme	synergistic	1053:1063	arg1	effect					1065:1070	a synergistic effect	1051:1070	a synergistic effect of the two cations	1051:1089	Results evidenced a synergistic effect of the two cations, affecting positively both the encapsulation efficiency and the ability of the alginate polymeric matrix to control the drug release.
28322959	5	50	dep	composition	721:731	arg1	i.e.					716:719	i.e.	716:719	i.e.	716:719	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	6	51	dep	in	930:931	arg1	vitro					933:937	vitro	933:937	vitro	933:937	The obtained beads were characterized and their in vitro release performances were assessed in conditions simulating the gastrointestinal environment.
28322959	8	52	theme	guluronic	1455:1463	arg1	moieties					1480:1487	both guluronic and mannuronic moieties	1450:1487	both guluronic and mannuronic moieties of alginate	1450:1499	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	3	53	theme	divalent	482:489	arg1	Zn2+					509:512	Zn2+	509:512	Zn2+	509:512	With the aim to deeply explore this possibility, in this research we investigated the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties.
28322959	3	53	theme	divalent	482:489	arg1	Ca2+					500:503	Ca2+	500:503	Ca2+	500:503	With the aim to deeply explore this possibility, in this research we investigated the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties.
28322959	3	53	theme	divalent	482:489	arg1	cations					491:497	two divalent cations	478:497	two divalent cations (Ca2+ and Zn2+)	478:513	With the aim to deeply explore this possibility, in this research we investigated the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties.
28322959	5	54	theme	main	657:660	arg1	variables					671:679	The main critical variables	653:679	The main critical variables	653:679	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	8	55	theme	covalent-like	1403:1415	arg1	bonds					1417:1421	covalent-like bonds	1403:1421	covalent-like bonds	1403:1421	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	5	56	dep	conditions	830:839	arg1	conditions					830:839	cross-linking conditions	816:839	cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+)	816:865	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	56	dep	conditions	830:839	arg1	Ca2++Zn2+					856:864	Ca2++Zn2+	856:864	Ca2++Zn2+	856:864	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	56	dep	conditions	830:839	arg1	Zn2+					848:851	Zn2+	848:851	Zn2+	848:851	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	56	dep	conditions	830:839	arg1	Ca2+					842:845	Ca2+	842:845	Ca2+	842:845	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	8	57	theme	Ca2+	1274:1277	arg1	ability					1279:1285	the Ca2+ ability	1270:1285	the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate	1270:1499	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	6	58	theme	gastrointestinal	1003:1018	arg1	environment					1020:1030	the gastrointestinal environment	999:1030	the gastrointestinal environment	999:1030	The obtained beads were characterized and their in vitro release performances were assessed in conditions simulating the gastrointestinal environment.
28322959	5	59	theme	critical	662:669	arg1	variables					671:679	The main critical variables	653:679	The main critical variables	653:679	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	8	60	theme	moieties	1480:1487	arg1	groups					1440:1445	carboxylate groups	1428:1445	carboxylate groups of both guluronic and mannuronic moieties of alginate	1428:1499	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	8	60	theme	moieties	1480:1487	arg1	moieties					1480:1487	both guluronic and mannuronic moieties	1450:1487	both guluronic and mannuronic moieties of alginate	1450:1499	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	2	61	theme	simultaneous	326:337	arg1	use					339:341	the simultaneous use	322:341	the simultaneous use of multiple cations as gelling agents	322:379	To date, only a few studies have focused on the simultaneous use of multiple cations as gelling agents.
28322959	8	62	theme	quicker	1300:1306	arg1	interactions					1322:1333	quicker electrostatic interactions	1300:1333	quicker electrostatic interactions with guluronic groups of alginate	1300:1367	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	8	63	theme	mannuronic	1469:1478	arg1	moieties					1480:1487	both guluronic and mannuronic moieties	1450:1487	both guluronic and mannuronic moieties of alginate	1450:1499	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	3	64	dep	beads	527:531	arg1	formation					533:541	formation	533:541	formation	533:541	With the aim to deeply explore this possibility, in this research we investigated the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties.
28322959	3	64	dep	beads	527:531	arg1	properties					547:556	properties	547:556	properties	547:556	With the aim to deeply explore this possibility, in this research we investigated the effect of two divalent cations (Ca2+ and Zn2+) on alginate beads formation and properties.
28322959	8	65	with	interactions	1322:1333	arg1	alginate					1360:1367	alginate	1360:1367	alginate	1360:1367	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	8	65	with	interactions	1322:1333	arg1	groups					1350:1355	guluronic groups	1340:1355	guluronic groups of alginate	1340:1367	A Ca2+/Zn2+ ratio of 4:1, in fact, exploited the Ca2+ ability of establish quicker electrostatic interactions with guluronic groups of alginate and the Zn2+ ability to establish covalent-like bonds with carboxylate groups of both guluronic and mannuronic moieties of alginate.
28322959	5	66	theme	sodium	764:769	arg1	solutions					753:761	the aqueous feed solutions	736:761	the aqueous feed solutions (sodium alginate and prednisolone concentration)	736:810	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	5	66	theme	sodium	764:769	arg1	alginate					771:778	sodium alginate	764:778	sodium alginate	764:778	The main critical variables of the ionotropic gelation process i.e. composition of the aqueous feed solutions (sodium alginate and prednisolone concentration) and cross-linking conditions (Ca2+, Zn2+ or Ca2++Zn2+), were studied.
28322959	6	67	theme	obtained	886:893	arg1	beads					895:899	The obtained beads	882:899	The obtained beads	882:899	The obtained beads were characterized and their in vitro release performances were assessed in conditions simulating the gastrointestinal environment.
28322959	2	68	theme	gelling	366:372	arg1	agents					374:379	gelling agents	366:379	gelling agents	366:379	To date, only a few studies have focused on the simultaneous use of multiple cations as gelling agents.
28322959	1	69	theme	product	269:275	arg1	characteristics					246:260	the technological characteristics	228:260	the technological characteristics of the product	228:275	Gelling solution parameters are some of the most important variables in ionotropic gelation and consequently influence the technological characteristics of the product.
25029999	0	0	theme	enhanced	90:97	arg1	activity					121:128	enhanced surface antibacterial activity	90:128	enhanced surface antibacterial activity	90:128	Hybrid nanostructured Ag/ZnO decorated powder cellulose fillers for medical plastics with enhanced surface antibacterial activity.
25029999	5	1	theme	powder	724:729	arg1	diffractometry					737:750	powder X-ray diffractometry	724:750	powder X-ray diffractometry	724:750	Scanning electron microscopy and powder X-ray diffractometry were used to investigate the morphology and crystalline phase structure of fillers.
25029999	1	2	theme	silver/zinc	188:198	arg1	decorations					206:216	nanostructured silver/zinc oxide decorations	173:216	nanostructured silver/zinc oxide decorations	173:216	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	7	3	theme	electrical	988:997	arg1	properties					1017:1026	electrical and antibacterial properties	988:1026	electrical and antibacterial properties	988:1026	With respect to prospective application, the composites were tested on electrical and antibacterial properties.
25029999	4	4	theme	poly	662:665	arg1	matrix					683:688	medical grade poly(vinyl chloride) matrix	648:688	medical grade poly(vinyl chloride) matrix	648:688	Prepared particulate fillers were compounded to medical grade poly(vinyl chloride) matrix.
25029999	5	5	theme	X-ray	731:735	arg1	diffractometry					737:750	powder X-ray diffractometry	724:750	powder X-ray diffractometry	724:750	Scanning electron microscopy and powder X-ray diffractometry were used to investigate the morphology and crystalline phase structure of fillers.
25029999	9	6	theme	water	1291:1295	arg1	soaking					1297:1303	water soaking	1291:1303	water soaking of composites	1291:1317	Electrical conductivity of fillers and composites was measured and no influence of water soaking of composites was found at all.
25029999	1	7	theme	oxide	200:204	arg1	decorations					206:216	nanostructured silver/zinc oxide decorations	173:216	nanostructured silver/zinc oxide decorations	173:216	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	4	8	theme	grade	656:660	arg1	poly					662:665	grade poly	656:665	medical grade poly(vinyl chloride) matrix	648:688	Prepared particulate fillers were compounded to medical grade poly(vinyl chloride) matrix.
25029999	4	8	theme	grade	656:660	arg1	chloride					673:680	vinyl chloride	667:680	vinyl chloride	667:680	Prepared particulate fillers were compounded to medical grade poly(vinyl chloride) matrix.
25029999	0	9	theme	antibacterial	107:119	arg1	activity					121:128	enhanced surface antibacterial activity	90:128	enhanced surface antibacterial activity	90:128	Hybrid nanostructured Ag/ZnO decorated powder cellulose fillers for medical plastics with enhanced surface antibacterial activity.
25029999	6	10	theme	composites	905:914	arg1	study					896:900	morphological study	882:900	morphological study of composites	882:914	The scanning electron microscopy was used for morphological study of composites.
25029999	1	11	theme	soluble	329:335	arg1	precursors					346:355	precursors	346:355	precursors of silver and zinc oxide	346:380	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	1	11	theme	soluble	329:335	arg1	salts					337:341	soluble salts	329:341	soluble salts	329:341	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	0	12	theme	surface	99:105	arg1	activity					121:128	enhanced surface antibacterial activity	90:128	enhanced surface antibacterial activity	90:128	Hybrid nanostructured Ag/ZnO decorated powder cellulose fillers for medical plastics with enhanced surface antibacterial activity.
25029999	9	13	theme	composites	1247:1256	arg1	conductivity					1219:1230	Electrical conductivity	1208:1230	Electrical conductivity of fillers and composites	1208:1256	Electrical conductivity of fillers and composites was measured and no influence of water soaking of composites was found at all.
25029999	0	14	with	plastics	76:83	arg1	activity					121:128	enhanced surface antibacterial activity	90:128	enhanced surface antibacterial activity	90:128	Hybrid nanostructured Ag/ZnO decorated powder cellulose fillers for medical plastics with enhanced surface antibacterial activity.
25029999	5	15	theme	fillers	827:833	arg1	structure					814:822	the morphology and crystalline phase structure	777:822	the morphology and crystalline phase structure of fillers	777:833	Scanning electron microscopy and powder X-ray diffractometry were used to investigate the morphology and crystalline phase structure of fillers.
25029999	5	16	theme	Scanning	691:698	arg1	microscopy					709:718	Scanning electron microscopy	691:718	Scanning electron microscopy	691:718	Scanning electron microscopy and powder X-ray diffractometry were used to investigate the morphology and crystalline phase structure of fillers.
25029999	3	17	theme	cellulose	537:545	arg1	particles					547:555	the cellulose particles	533:555	the cellulose particles	533:555	The inorganics covered all available surfaces of the cellulose particles with a morphology resembling a coral reef.
25029999	10	18	theme	ISO	1435:1437	arg1	22196					1439:1443	the standard ISO 22196	1422:1443	the standard ISO 22196	1422:1443	The surface antibacterial activity of prepared composites was evaluated according to the standard ISO 22196.
25029999	5	19	theme	electron	700:707	arg1	microscopy					709:718	Scanning electron microscopy	691:718	Scanning electron microscopy	691:718	Scanning electron microscopy and powder X-ray diffractometry were used to investigate the morphology and crystalline phase structure of fillers.
25029999	6	20	theme	morphological	882:894	arg1	study					896:900	morphological study	882:900	morphological study of composites	882:914	The scanning electron microscopy was used for morphological study of composites.
25029999	9	21	located	found	1323:1327	arg2	influence					1278:1286	no influence	1275:1286	no influence of water soaking of composites	1275:1317	Electrical conductivity of fillers and composites was measured and no influence of water soaking of composites was found at all.
25029999	9	21	located	found	1323:1327	arg1	all					1332:1334	all	1332:1334	all	1332:1334	Electrical conductivity of fillers and composites was measured and no influence of water soaking of composites was found at all.
25029999	3	22	theme	available	511:519	arg1	surfaces					521:528	all available surfaces	507:528	all available surfaces of the cellulose particles	507:555	The inorganics covered all available surfaces of the cellulose particles with a morphology resembling a coral reef.
25029999	0	23	theme	nanostructured	7:20	arg1	Ag/ZnO					22:27	Hybrid nanostructured Ag/ZnO	0:27	Hybrid nanostructured Ag/ZnO	0:27	Hybrid nanostructured Ag/ZnO decorated powder cellulose fillers for medical plastics with enhanced surface antibacterial activity.
25029999	8	24	theme	prepared	1173:1180	arg1	materials					1182:1190	prepared materials	1173:1190	prepared materials	1173:1190	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	1	25	theme	micro-cellulose	221:235	arg1	particles					245:253	micro-cellulose carrier particles	221:253	micro-cellulose carrier particles	221:253	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	0	26	theme	Hybrid	0:5	arg1	Ag/ZnO					22:27	Hybrid nanostructured Ag/ZnO	0:27	Hybrid nanostructured Ag/ZnO	0:27	Hybrid nanostructured Ag/ZnO decorated powder cellulose fillers for medical plastics with enhanced surface antibacterial activity.
25029999	2	27	used	used	410:413	arg2	Hexamethylenetetramine					383:404	Hexamethylenetetramine	383:404	Hexamethylenetetramine	383:404	Hexamethylenetetramine was used as precipitating agent for zinc oxide and reducing agent for silver.
25029999	8	28	theme	exposure	1161:1168	arg1	effect					1145:1150	no adverse effect	1134:1150	no adverse effect of water exposure on prepared materials	1134:1190	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	9	29	theme	Electrical	1208:1217	arg1	conductivity					1219:1230	Electrical conductivity	1208:1230	Electrical conductivity of fillers and composites	1208:1256	Electrical conductivity of fillers and composites was measured and no influence of water soaking of composites was found at all.
25029999	1	30	theme	carrier	237:243	arg1	particles					245:253	micro-cellulose carrier particles	221:253	micro-cellulose carrier particles	221:253	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	8	31	from	effect	1037:1042	arg1	composites					1075:1084	polymer composites	1067:1084	polymer composites	1067:1084	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	8	31	from	effect	1037:1042	arg1	properties					1106:1115	their dielectric properties	1089:1115	their dielectric properties	1089:1115	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	5	32	theme	morphology	781:790	arg1	structure					814:822	the morphology and crystalline phase structure	777:822	the morphology and crystalline phase structure of fillers	777:833	Scanning electron microscopy and powder X-ray diffractometry were used to investigate the morphology and crystalline phase structure of fillers.
25029999	9	33	theme	composites	1308:1317	arg1	soaking					1297:1303	water soaking	1291:1303	water soaking of composites	1291:1317	Electrical conductivity of fillers and composites was measured and no influence of water soaking of composites was found at all.
25029999	7	34	theme	prospective	933:943	arg1	application					945:955	prospective application	933:955	prospective application	933:955	With respect to prospective application, the composites were tested on electrical and antibacterial properties.
25029999	5	35	used	used	757:760	arg2	diffractometry					737:750	powder X-ray diffractometry	724:750	powder X-ray diffractometry	724:750	Scanning electron microscopy and powder X-ray diffractometry were used to investigate the morphology and crystalline phase structure of fillers.
25029999	5	35	used	used	757:760	arg2	microscopy					709:718	Scanning electron microscopy	691:718	Scanning electron microscopy	691:718	Scanning electron microscopy and powder X-ray diffractometry were used to investigate the morphology and crystalline phase structure of fillers.
25029999	4	36	theme	medical	648:654	arg1	matrix					683:688	medical grade poly(vinyl chloride) matrix	648:688	medical grade poly(vinyl chloride) matrix	648:688	Prepared particulate fillers were compounded to medical grade poly(vinyl chloride) matrix.
25029999	9	37	theme	fillers	1235:1241	arg1	conductivity					1219:1230	Electrical conductivity	1208:1230	Electrical conductivity of fillers and composites	1208:1256	Electrical conductivity of fillers and composites was measured and no influence of water soaking of composites was found at all.
25029999	10	38	theme	standard	1426:1433	arg1	22196					1439:1443	the standard ISO 22196	1422:1443	the standard ISO 22196	1422:1443	The surface antibacterial activity of prepared composites was evaluated according to the standard ISO 22196.
25029999	3	39	theme	coral	588:592	arg1	reef					594:597	a coral reef	586:597	a coral reef	586:597	The inorganics covered all available surfaces of the cellulose particles with a morphology resembling a coral reef.
25029999	0	40	theme	cellulose	46:54	arg1	fillers					56:62	powder cellulose fillers	39:62	powder cellulose fillers for medical plastics with enhanced surface antibacterial activity	39:128	Hybrid nanostructured Ag/ZnO decorated powder cellulose fillers for medical plastics with enhanced surface antibacterial activity.
25029999	1	41	theme	silver	360:365	arg1	precursors					346:355	precursors	346:355	precursors of silver and zinc oxide	346:380	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	1	41	theme	silver	360:365	arg1	salts					337:341	soluble salts	329:341	soluble salts	329:341	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	5	42	theme	crystalline	796:806	arg1	structure					814:822	the morphology and crystalline phase structure	777:822	the morphology and crystalline phase structure of fillers	777:833	Scanning electron microscopy and powder X-ray diffractometry were used to investigate the morphology and crystalline phase structure of fillers.
25029999	7	43	theme	antibacterial	1003:1015	arg1	properties					1017:1026	electrical and antibacterial properties	988:1026	electrical and antibacterial properties	988:1026	With respect to prospective application, the composites were tested on electrical and antibacterial properties.
25029999	8	44	from	absorption	1053:1062	arg1	composites					1075:1084	polymer composites	1067:1084	polymer composites	1067:1084	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	0	45	theme	powder	39:44	arg1	fillers					56:62	powder cellulose fillers	39:62	powder cellulose fillers for medical plastics with enhanced surface antibacterial activity	39:128	Hybrid nanostructured Ag/ZnO decorated powder cellulose fillers for medical plastics with enhanced surface antibacterial activity.
25029999	4	46	theme	Prepared	600:607	arg1	fillers					621:627	Prepared particulate fillers	600:627	Prepared particulate fillers	600:627	Prepared particulate fillers were compounded to medical grade poly(vinyl chloride) matrix.
25029999	5	47	theme	phase	808:812	arg1	structure					814:822	the morphology and crystalline phase structure	777:822	the morphology and crystalline phase structure of fillers	777:833	Scanning electron microscopy and powder X-ray diffractometry were used to investigate the morphology and crystalline phase structure of fillers.
25029999	10	48	theme	prepared	1375:1382	arg1	composites					1384:1393	prepared composites	1375:1393	prepared composites	1375:1393	The surface antibacterial activity of prepared composites was evaluated according to the standard ISO 22196.
25029999	7	49	dep	application	945:955	arg1	respect					922:928	respect	922:928	respect	922:928	With respect to prospective application, the composites were tested on electrical and antibacterial properties.
25029999	11	50	theme	Excellent	1446:1454	arg1	performance					1456:1466	Excellent performance	1446:1466	Excellent performance against Escherichia coli and very high against Staphylococcus aureus	1446:1535	Excellent performance against Escherichia coli and very high against Staphylococcus aureus was achieved.
25029999	1	51	theme	zinc	371:374	arg1	oxide					376:380	zinc oxide	371:380	zinc oxide	371:380	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	8	52	from	effect	1145:1150	arg1	materials					1182:1190	prepared materials	1173:1190	prepared materials	1173:1190	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	6	53	theme	electron	849:856	arg1	microscopy					858:867	The scanning electron microscopy	836:867	The scanning electron microscopy	836:867	The scanning electron microscopy was used for morphological study of composites.
25029999	1	54	theme	Hybrid	131:136	arg1	fillers					156:162	Hybrid inorganic-organic fillers	131:162	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles	131:253	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	1	55	theme	oxide	376:380	arg1	precursors					346:355	precursors	346:355	precursors of silver and zinc oxide	346:380	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	1	55	theme	oxide	376:380	arg1	salts					337:341	soluble salts	329:341	soluble salts	329:341	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	4	56	theme	vinyl	667:671	arg1	poly					662:665	grade poly	656:665	medical grade poly(vinyl chloride) matrix	648:688	Prepared particulate fillers were compounded to medical grade poly(vinyl chloride) matrix.
25029999	4	56	theme	vinyl	667:671	arg1	chloride					673:680	vinyl chloride	667:680	vinyl chloride	667:680	Prepared particulate fillers were compounded to medical grade poly(vinyl chloride) matrix.
25029999	8	57	theme	water	1155:1159	arg1	exposure					1161:1168	water exposure	1155:1168	water exposure	1155:1168	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	6	58	theme	scanning	840:847	arg1	microscopy					858:867	The scanning electron microscopy	836:867	The scanning electron microscopy	836:867	The scanning electron microscopy was used for morphological study of composites.
25029999	1	59	theme	inorganic-organic	138:154	arg1	fillers					156:162	Hybrid inorganic-organic fillers	131:162	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles	131:253	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	10	60	theme	antibacterial	1349:1361	arg1	activity					1363:1370	The surface antibacterial activity	1337:1370	The surface antibacterial activity of prepared composites	1337:1393	The surface antibacterial activity of prepared composites was evaluated according to the standard ISO 22196.
25029999	4	61	theme	particulate	609:619	arg1	fillers					621:627	Prepared particulate fillers	600:627	Prepared particulate fillers	600:627	Prepared particulate fillers were compounded to medical grade poly(vinyl chloride) matrix.
25029999	8	62	theme	dielectric	1095:1104	arg1	properties					1106:1115	their dielectric properties	1089:1115	their dielectric properties	1089:1115	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	9	63	theme	soaking	1297:1303	arg1	influence					1278:1286	no influence	1275:1286	no influence of water soaking of composites	1275:1317	Electrical conductivity of fillers and composites was measured and no influence of water soaking of composites was found at all.
25029999	3	64	theme	particles	547:555	arg1	surfaces					521:528	all available surfaces	507:528	all available surfaces of the cellulose particles	507:555	The inorganics covered all available surfaces of the cellulose particles with a morphology resembling a coral reef.
25029999	10	65	theme	surface	1341:1347	arg1	activity					1363:1370	The surface antibacterial activity	1337:1370	The surface antibacterial activity of prepared composites	1337:1393	The surface antibacterial activity of prepared composites was evaluated according to the standard ISO 22196.
25029999	1	66	theme	stepwise	272:279	arg1	synthesis					313:321	stepwise microwave assisted hydrothermal synthesis	272:321	stepwise microwave assisted hydrothermal synthesis	272:321	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	8	67	theme	small	1031:1035	arg1	effect					1037:1042	A small effect	1029:1042	A small effect of water absorption in polymer composites on their dielectric properties	1029:1115	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	8	68	theme	absorption	1053:1062	arg1	effect					1037:1042	A small effect	1029:1042	A small effect of water absorption in polymer composites on their dielectric properties	1029:1115	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	8	69	from	composites	1075:1084	arg1	effect					1037:1042	A small effect	1029:1042	A small effect of water absorption in polymer composites on their dielectric properties	1029:1115	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	1	70	theme	microwave	281:289	arg1	synthesis					313:321	stepwise microwave assisted hydrothermal synthesis	272:321	stepwise microwave assisted hydrothermal synthesis	272:321	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	8	71	theme	water	1047:1051	arg1	absorption					1053:1062	water absorption	1047:1062	water absorption in polymer composites	1047:1084	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	2	72	theme	zinc	442:445	arg1	oxide					447:451	zinc oxide	442:451	zinc oxide	442:451	Hexamethylenetetramine was used as precipitating agent for zinc oxide and reducing agent for silver.
25029999	6	73	used	used	873:876	arg2	microscopy					858:867	The scanning electron microscopy	836:867	The scanning electron microscopy	836:867	The scanning electron microscopy was used for morphological study of composites.
25029999	1	74	theme	assisted	291:298	arg1	synthesis					313:321	stepwise microwave assisted hydrothermal synthesis	272:321	stepwise microwave assisted hydrothermal synthesis	272:321	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	0	75	theme	medical	68:74	arg1	plastics					76:83	medical plastics	68:83	medical plastics with enhanced surface antibacterial activity	68:128	Hybrid nanostructured Ag/ZnO decorated powder cellulose fillers for medical plastics with enhanced surface antibacterial activity.
25029999	8	76	theme	adverse	1137:1143	arg1	effect					1145:1150	no adverse effect	1134:1150	no adverse effect of water exposure on prepared materials	1134:1190	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	8	77	theme	polymer	1067:1073	arg1	composites					1075:1084	polymer composites	1067:1084	polymer composites	1067:1084	A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested.
25029999	1	78	theme	nanostructured	173:186	arg1	decorations					206:216	nanostructured silver/zinc oxide decorations	173:216	nanostructured silver/zinc oxide decorations	173:216	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
25029999	10	79	theme	composites	1384:1393	arg1	activity					1363:1370	The surface antibacterial activity	1337:1370	The surface antibacterial activity of prepared composites	1337:1393	The surface antibacterial activity of prepared composites was evaluated according to the standard ISO 22196.
25029999	1	80	theme	hydrothermal	300:311	arg1	synthesis					313:321	stepwise microwave assisted hydrothermal synthesis	272:321	stepwise microwave assisted hydrothermal synthesis	272:321	Hybrid inorganic-organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide.
29130419	11	0	theme	many	1520:1523	arg1	similarities					1525:1536	many similarities	1520:1536	many similarities	1520:1536	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	11	0	theme	many	1520:1523	arg1	proliferation					1614:1626	epidermal basal layer proliferation	1592:1626	epidermal basal layer proliferation	1592:1626	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	11	0	theme	many	1520:1523	arg1	formation					1581:1589	BM formation	1578:1589	BM formation	1578:1589	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	11	0	theme	many	1520:1523	arg1	structure					1567:1575	the dermal matrix structure	1549:1575	the dermal matrix structure	1549:1575	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	11	0	theme	many	1520:1523	arg1	execution					1633:1641	execution	1633:1641	execution of differentiation programs	1633:1669	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	12	1	contain	contains	1679:1686	arg2	number					1698:1703	a similar number	1688:1703	a similar number of corneocyte layers	1688:1724	The SC contains a similar number of corneocyte layers and the same level of lipids.
29130419	12	1	contain	contains	1679:1686	arg1	SC					1676:1677	The SC	1672:1677	The SC	1672:1677	The SC contains a similar number of corneocyte layers and the same level of lipids.
29130419	12	1	contain	contains	1679:1686	arg2	level					1739:1743	the same level	1730:1743	the same level of lipids	1730:1753	The SC contains a similar number of corneocyte layers and the same level of lipids.
29130419	6	2	theme	human	993:997	arg1	dermis					1009:1014	human abdominal dermis	993:1014	human abdominal dermis	993:1014	MATERIALS AND METHODS An isolation procedure to obtain soluble collagen from human abdominal dermis was developed.
29130419	11	3	theme	matrix	1560:1565	arg1	structure					1567:1575	the dermal matrix structure	1549:1575	the dermal matrix structure	1549:1575	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	5	4	theme	collagen	744:751	arg1	replacement					714:724	replacement	714:724	replacement of animal-derived collagen for human collagen	714:770	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	9	5	theme	corneum	1317:1323	arg1	composition					1336:1346	The stratum corneum (SC) lipid composition	1305:1346	The stratum corneum (SC) lipid composition	1305:1346	The stratum corneum (SC) lipid composition was studied with liquid chromatography-mass spectrometry.
29130419	15	6	theme	abdominal	2023:2031	arg1	dermis					2033:2038	human abdominal dermis	2017:2038	human abdominal dermis	2017:2038	CONCLUSION The animal material-free hC-FTM is generated successfully using collagens isolated from human abdominal dermis.
29130419	15	7	dep	CONCLUSION	1918:1927	arg1	generated					1964:1972	generated	1964:1972	is generated successfully using collagens isolated from human abdominal dermis	1961:2038	CONCLUSION The animal material-free hC-FTM is generated successfully using collagens isolated from human abdominal dermis.
29130419	8	8	with	analyses	1138:1145	arg1	biomarkers					1152:1161	biomarkers	1152:1161	biomarkers for the dermal matrix composition	1152:1195	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	8	8	with	analyses	1138:1145	arg1	activation					1278:1287	activation	1278:1287	activation	1278:1287	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	8	8	with	analyses	1138:1145	arg1	differentiation					1257:1271	differentiation	1257:1271	differentiation	1257:1271	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	8	8	with	analyses	1138:1145	arg1	formation					1221:1229	basement membrane (BM) formation	1198:1229	basement membrane (BM) formation	1198:1229	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	8	8	with	analyses	1138:1145	arg1	proliferation					1242:1254	epidermal proliferation	1232:1254	epidermal proliferation	1232:1254	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	1	9	theme	animal-derived	195:208	arg1	matrix					219:224	an animal-derived collagen matrix	192:224	an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes	192:301	OBJECTIVE Full-thickness skin models comprise a three-dimensional dermal equivalent based on an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes.
29130419	9	10	theme	lipid	1330:1334	arg1	composition					1336:1346	The stratum corneum (SC) lipid composition	1305:1346	The stratum corneum (SC) lipid composition	1305:1346	The stratum corneum (SC) lipid composition was studied with liquid chromatography-mass spectrometry.
29130419	11	11	theme	basal	1602:1606	arg1	proliferation					1614:1626	epidermal basal layer proliferation	1592:1626	epidermal basal layer proliferation	1592:1626	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	12	12	theme	lipids	1748:1753	arg1	number					1698:1703	a similar number	1688:1703	a similar number of corneocyte layers	1688:1724	The SC contains a similar number of corneocyte layers and the same level of lipids.
29130419	12	12	theme	lipids	1748:1753	arg1	level					1739:1743	the same level	1730:1743	the same level of lipids	1730:1753	The SC contains a similar number of corneocyte layers and the same level of lipids.
29130419	5	13	theme	dermal	901:906	arg1	tissue					908:913	native dermal tissue	894:913	native dermal tissue	894:913	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	12	14	theme	layers	1719:1724	arg1	number					1698:1703	a similar number	1688:1703	a similar number of corneocyte layers	1688:1724	The SC contains a similar number of corneocyte layers and the same level of lipids.
29130419	12	14	theme	layers	1719:1724	arg1	level					1739:1743	the same level	1730:1743	the same level of lipids	1730:1753	The SC contains a similar number of corneocyte layers and the same level of lipids.
29130419	5	15	theme	human	757:761	arg1	collagen					763:770	human collagen	757:770	human collagen	757:770	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	1	16	theme	skin	124:127	arg1	models					129:134	OBJECTIVE Full-thickness skin models	99:134	OBJECTIVE Full-thickness skin models	99:134	OBJECTIVE Full-thickness skin models comprise a three-dimensional dermal equivalent based on an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes.
29130419	9	17	theme	chromatography-mass	1372:1390	arg1	spectrometry					1392:1403	liquid chromatography-mass spectrometry	1365:1403	liquid chromatography-mass spectrometry	1365:1403	The stratum corneum (SC) lipid composition was studied with liquid chromatography-mass spectrometry.
29130419	7	18	theme	human	1081:1085	arg1	fibroblasts					1087:1097	primary human fibroblasts	1073:1097	primary human fibroblasts	1073:1097	Both FTMs and hC-FTMs were generated with primary human fibroblasts and keratinocytes.
29130419	15	19	theme	material-free	1940:1952	arg1	hC-FTM					1954:1959	The animal material-free hC-FTM	1929:1959	The animal material-free hC-FTM	1929:1959	CONCLUSION The animal material-free hC-FTM is generated successfully using collagens isolated from human abdominal dermis.
29130419	12	20	theme	similar	1690:1696	arg1	number					1698:1703	a similar number	1688:1703	a similar number of corneocyte layers	1688:1724	The SC contains a similar number of corneocyte layers and the same level of lipids.
29130419	5	21	theme	full-thickness	844:857	arg1	hC-FTMs					867:873	hC-FTMs	867:873	hC-FTMs	867:873	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	5	21	theme	full-thickness	844:857	arg1	models					859:864	the animal material-free human collagen full-thickness models	804:864	the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue	804:913	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	1	22	theme	three-dimensional	147:163	arg1	equivalent					172:181	a three-dimensional dermal equivalent	145:181	a three-dimensional dermal equivalent based on an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes	145:301	OBJECTIVE Full-thickness skin models comprise a three-dimensional dermal equivalent based on an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes.
29130419	18	23	theme	in	2288:2289	arg1	models					2302:2307	Therefore these in vitro skin models	2272:2307	Therefore these in vitro skin models	2272:2307	Therefore these in vitro skin models can be used as promising tool for research purposes that contribute to animal-free experimentation.
29130419	18	23	theme	in	2288:2289	arg1	tool					2334:2337	promising tool	2324:2337	promising tool for research purposes that contribute to animal-free experimentation	2324:2406	Therefore these in vitro skin models can be used as promising tool for research purposes that contribute to animal-free experimentation.
29130419	18	24	theme	skin	2297:2300	arg1	models					2302:2307	Therefore these in vitro skin models	2272:2307	Therefore these in vitro skin models	2272:2307	Therefore these in vitro skin models can be used as promising tool for research purposes that contribute to animal-free experimentation.
29130419	18	24	theme	skin	2297:2300	arg1	tool					2334:2337	promising tool	2324:2337	promising tool for research purposes that contribute to animal-free experimentation	2324:2406	Therefore these in vitro skin models can be used as promising tool for research purposes that contribute to animal-free experimentation.
29130419	17	25	theme	native	2230:2235	arg1	tissue					2245:2250	the native stromal tissue	2226:2250	the native stromal tissue	2226:2250	The hC-FTMs contain a dermal equivalent that mimics the native stromal tissue to a higher extent.
29130419	0	26	from	Recapitulation	0:13	arg1	Model					70:74	a Full-Thickness Human Skin Model	42:74	a Full-Thickness Human Skin Model	42:74	Recapitulation of Native Dermal Tissue in a Full-Thickness Human Skin Model Using Human Collagens.
29130419	11	27	theme	differentiation	1646:1660	arg1	programs					1662:1669	differentiation programs	1646:1669	differentiation programs	1646:1669	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	0	28	theme	Human	82:86	arg1	Collagens					88:96	Human Collagens	82:96	Human Collagens	82:96	Recapitulation of Native Dermal Tissue in a Full-Thickness Human Skin Model Using Human Collagens.
29130419	10	29	theme	lamellar	1412:1419	arg1	organization					1421:1432	Lipid lamellar organization	1406:1432	Lipid lamellar organization	1406:1432	Lipid lamellar organization was determined by small-angle X-ray diffraction.
29130419	3	30	theme	acid	481:484	arg1	composition					486:496	amino acid composition	475:496	amino acid composition	475:496	Animal-derived collagens differ in amino acid composition and physicochemical properties from human collagens.
29130419	5	31	theme	human	829:833	arg1	hC-FTMs					867:873	hC-FTMs	867:873	hC-FTMs	867:873	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	5	31	theme	human	829:833	arg1	models					859:864	the animal material-free human collagen full-thickness models	804:864	the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue	804:913	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	16	32	theme	original	2159:2166	arg1	FTMs					2168:2171	original FTMs	2159:2171	original FTMs	2159:2171	Utilization of human collagens revealed that (epi-)dermal morphogenesis and lipid barrier formation resembled that of original FTMs.
29130419	0	33	theme	Skin	65:68	arg1	Model					70:74	a Full-Thickness Human Skin Model	42:74	a Full-Thickness Human Skin Model	42:74	Recapitulation of Native Dermal Tissue in a Full-Thickness Human Skin Model Using Human Collagens.
29130419	2	34	theme	resident	420:427	arg1	type					434:437	resident cell type	420:437	resident cell type	420:437	The functionality of both equivalents is influenced by many factors, including extracellular matrix composition and resident cell type.
29130419	5	35	theme	native	894:899	arg1	tissue					908:913	native dermal tissue	894:913	native dermal tissue	894:913	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	8	36	theme	matrix	1178:1183	arg1	composition					1185:1195	the dermal matrix composition	1167:1195	the dermal matrix composition	1167:1195	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	4	37	theme	equivalent	612:621	arg1	functionality					584:596	the functionality	580:596	the functionality of the dermal equivalent	580:621	This composition could alter the functionality of the dermal equivalent and epidermal morphogenesis with the barrier formation in full-thickness models (FTMs).
29130419	4	37	theme	equivalent	612:621	arg1	morphogenesis					637:649	epidermal morphogenesis	627:649	epidermal morphogenesis	627:649	This composition could alter the functionality of the dermal equivalent and epidermal morphogenesis with the barrier formation in full-thickness models (FTMs).
29130419	11	38	theme	layer	1608:1612	arg1	proliferation					1614:1626	epidermal basal layer proliferation	1592:1626	epidermal basal layer proliferation	1592:1626	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	5	39	link	animal-derived	729:742	arg1	collagen					744:751	animal-derived collagen	729:751	animal-derived collagen	729:751	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	0	40	theme	Native	18:23	arg1	Tissue					32:37	Native Dermal Tissue	18:37	Native Dermal Tissue	18:37	Recapitulation of Native Dermal Tissue in a Full-Thickness Human Skin Model Using Human Collagens.
29130419	1	41	theme	OBJECTIVE	99:107	arg1	models					129:134	OBJECTIVE Full-thickness skin models	99:134	OBJECTIVE Full-thickness skin models	99:134	OBJECTIVE Full-thickness skin models comprise a three-dimensional dermal equivalent based on an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes.
29130419	4	42	theme	epidermal	627:635	arg1	morphogenesis					637:649	epidermal morphogenesis	627:649	epidermal morphogenesis	627:649	This composition could alter the functionality of the dermal equivalent and epidermal morphogenesis with the barrier formation in full-thickness models (FTMs).
29130419	13	43	theme	ceramide	1799:1806	arg1	profile					1817:1823	ceramide subclass profile	1799:1823	ceramide subclass profile	1799:1823	The ceramide chain length distribution and ceramide subclass profile showed only minor differences.
29130419	11	44	theme	epidermal	1592:1600	arg1	proliferation					1614:1626	epidermal basal layer proliferation	1592:1626	epidermal basal layer proliferation	1592:1626	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	0	45	theme	Tissue	32:37	arg1	Recapitulation					0:13	Recapitulation	0:13	Recapitulation of Native Dermal Tissue in a Full-Thickness Human Skin Model	0:74	Recapitulation of Native Dermal Tissue in a Full-Thickness Human Skin Model Using Human Collagens.
29130419	8	46	theme	membrane	1207:1214	arg1	biomarkers					1152:1161	biomarkers	1152:1161	biomarkers for the dermal matrix composition	1152:1195	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	8	46	theme	membrane	1207:1214	arg1	formation					1221:1229	basement membrane (BM) formation	1198:1229	basement membrane (BM) formation	1198:1229	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	6	47	theme	isolation	941:949	arg1	procedure					951:959	An isolation procedure	938:959	An isolation procedure	938:959	MATERIALS AND METHODS An isolation procedure to obtain soluble collagen from human abdominal dermis was developed.
29130419	0	48	theme	Human	59:63	arg1	Model					70:74	a Full-Thickness Human Skin Model	42:74	a Full-Thickness Human Skin Model	42:74	Recapitulation of Native Dermal Tissue in a Full-Thickness Human Skin Model Using Human Collagens.
29130419	13	49	theme	chain	1769:1773	arg1	distribution					1782:1793	The ceramide chain length distribution	1756:1793	The ceramide chain length distribution	1756:1793	The ceramide chain length distribution and ceramide subclass profile showed only minor differences.
29130419	18	50	dep	in	2288:2289	arg1	vitro					2291:2295	vitro	2291:2295	vitro	2291:2295	Therefore these in vitro skin models can be used as promising tool for research purposes that contribute to animal-free experimentation.
29130419	10	51	theme	X-ray	1464:1468	arg1	diffraction					1470:1480	small-angle X-ray diffraction	1452:1480	small-angle X-ray diffraction	1452:1480	Lipid lamellar organization was determined by small-angle X-ray diffraction.
29130419	2	52	theme	many	359:362	arg1	factors					364:370	many factors	359:370	many factors	359:370	The functionality of both equivalents is influenced by many factors, including extracellular matrix composition and resident cell type.
29130419	2	52	theme	many	359:362	arg1	type					434:437	resident cell type	420:437	resident cell type	420:437	The functionality of both equivalents is influenced by many factors, including extracellular matrix composition and resident cell type.
29130419	2	52	theme	many	359:362	arg1	composition					404:414	extracellular matrix composition	383:414	extracellular matrix composition	383:414	The functionality of both equivalents is influenced by many factors, including extracellular matrix composition and resident cell type.
29130419	16	53	theme	lipid	2117:2121	arg1	formation					2131:2139	lipid barrier formation	2117:2139	lipid barrier formation	2117:2139	Utilization of human collagens revealed that (epi-)dermal morphogenesis and lipid barrier formation resembled that of original FTMs.
29130419	17	54	contain	contain	2186:2192	arg1	hC-FTMs					2178:2184	The hC-FTMs	2174:2184	The hC-FTMs	2174:2184	The hC-FTMs contain a dermal equivalent that mimics the native stromal tissue to a higher extent.
29130419	17	54	contain	contain	2186:2192	arg2	equivalent					2203:2212	a dermal equivalent	2194:2212	a dermal equivalent that mimics the native stromal tissue to a higher extent	2194:2269	The hC-FTMs contain a dermal equivalent that mimics the native stromal tissue to a higher extent.
29130419	18	55	theme	animal-free	2380:2390	arg1	experimentation					2392:2406	animal-free experimentation	2380:2406	animal-free experimentation	2380:2406	Therefore these in vitro skin models can be used as promising tool for research purposes that contribute to animal-free experimentation.
29130419	14	56	theme	lamellar	1895:1902	arg1	organization					1904:1915	an unaltered lamellar organization	1882:1915	an unaltered lamellar organization	1882:1915	Subsequently, this led to an unaltered lamellar organization.
29130419	8	57	theme	epidermal	1232:1240	arg1	biomarkers					1152:1161	biomarkers	1152:1161	biomarkers for the dermal matrix composition	1152:1195	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	8	57	theme	epidermal	1232:1240	arg1	proliferation					1242:1254	epidermal proliferation	1232:1254	epidermal proliferation	1232:1254	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	15	58	theme	human	2017:2021	arg1	dermis					2033:2038	human abdominal dermis	2017:2038	human abdominal dermis	2017:2038	CONCLUSION The animal material-free hC-FTM is generated successfully using collagens isolated from human abdominal dermis.
29130419	2	59	theme	extracellular	383:395	arg1	composition					404:414	extracellular matrix composition	383:414	extracellular matrix composition	383:414	The functionality of both equivalents is influenced by many factors, including extracellular matrix composition and resident cell type.
29130419	6	60	theme	abdominal	999:1007	arg1	dermis					1009:1014	human abdominal dermis	993:1014	human abdominal dermis	993:1014	MATERIALS AND METHODS An isolation procedure to obtain soluble collagen from human abdominal dermis was developed.
29130419	11	61	theme	dermal	1553:1558	arg1	structure					1567:1575	the dermal matrix structure	1549:1575	the dermal matrix structure	1549:1575	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	5	62	theme	animal-derived	729:742	arg1	collagen					744:751	animal-derived collagen	729:751	animal-derived collagen	729:751	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	11	63	dep	RESULTS	1483:1489	arg1	FTMs					1495:1498	FTMs	1495:1498	FTMs	1495:1498	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	11	63	dep	RESULTS	1483:1489	arg1	hC-FTMs					1504:1510	hC-FTMs	1504:1510	hC-FTMs	1504:1510	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	4	64	theme	barrier	660:666	arg1	formation					668:676	the barrier formation	656:676	the barrier formation in full-thickness models (FTMs)	656:708	This composition could alter the functionality of the dermal equivalent and epidermal morphogenesis with the barrier formation in full-thickness models (FTMs).
29130419	9	65	theme	stratum	1309:1315	arg1	SC					1326:1327	SC	1326:1327	SC	1326:1327	The stratum corneum (SC) lipid composition was studied with liquid chromatography-mass spectrometry.
29130419	9	65	theme	stratum	1309:1315	arg1	corneum					1317:1323	stratum corneum	1309:1323	The stratum corneum (SC) lipid composition	1305:1346	The stratum corneum (SC) lipid composition was studied with liquid chromatography-mass spectrometry.
29130419	6	66	theme	soluble	971:977	arg1	collagen					979:986	soluble collagen	971:986	soluble collagen	971:986	MATERIALS AND METHODS An isolation procedure to obtain soluble collagen from human abdominal dermis was developed.
29130419	11	67	dep	FTMs	1495:1498	arg1	The					1491:1493	The	1491:1493	The	1491:1493	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	3	68	from	collagens	540:548	arg1	composition					486:496	amino acid composition	475:496	amino acid composition	475:496	Animal-derived collagens differ in amino acid composition and physicochemical properties from human collagens.
29130419	3	68	from	collagens	540:548	arg1	properties					518:527	physicochemical properties	502:527	physicochemical properties	502:527	Animal-derived collagens differ in amino acid composition and physicochemical properties from human collagens.
29130419	16	69	theme	collagens	2062:2070	arg1	Utilization					2041:2051	Utilization	2041:2051	Utilization of human collagens	2041:2070	Utilization of human collagens revealed that (epi-)dermal morphogenesis and lipid barrier formation resembled that of original FTMs.
29130419	18	70	theme	research	2343:2350	arg1	purposes					2352:2359	research purposes	2343:2359	research purposes that contribute to animal-free experimentation	2343:2406	Therefore these in vitro skin models can be used as promising tool for research purposes that contribute to animal-free experimentation.
29130419	8	71	theme	Immunohistochemical	1118:1136	arg1	analyses					1138:1145	Immunohistochemical analyses	1118:1145	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation	1118:1287	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	17	72	theme	higher	2257:2262	arg1	extent					2264:2269	a higher extent	2255:2269	a higher extent	2255:2269	The hC-FTMs contain a dermal equivalent that mimics the native stromal tissue to a higher extent.
29130419	2	73	theme	equivalents	330:340	arg1	functionality					308:320	The functionality	304:320	The functionality of both equivalents	304:340	The functionality of both equivalents is influenced by many factors, including extracellular matrix composition and resident cell type.
29130419	1	74	theme	collagen	210:217	arg1	matrix					219:224	an animal-derived collagen matrix	192:224	an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes	192:301	OBJECTIVE Full-thickness skin models comprise a three-dimensional dermal equivalent based on an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes.
29130419	18	75	used	used	2316:2319	arg2	models					2302:2307	Therefore these in vitro skin models	2272:2307	Therefore these in vitro skin models	2272:2307	Therefore these in vitro skin models can be used as promising tool for research purposes that contribute to animal-free experimentation.
29130419	18	75	used	used	2316:2319	arg2	tool					2334:2337	promising tool	2324:2337	promising tool for research purposes that contribute to animal-free experimentation	2324:2406	Therefore these in vitro skin models can be used as promising tool for research purposes that contribute to animal-free experimentation.
29130419	16	76	theme	human	2056:2060	arg1	collagens					2062:2070	human collagens	2056:2070	human collagens	2056:2070	Utilization of human collagens revealed that (epi-)dermal morphogenesis and lipid barrier formation resembled that of original FTMs.
29130419	16	77	theme	epi-	2087:2090	arg1	morphogenesis					2099:2111	(epi-)dermal morphogenesis	2086:2111	(epi-)dermal morphogenesis	2086:2111	Utilization of human collagens revealed that (epi-)dermal morphogenesis and lipid barrier formation resembled that of original FTMs.
29130419	1	78	theme	Full-thickness	109:122	arg1	models					129:134	OBJECTIVE Full-thickness skin models	99:134	OBJECTIVE Full-thickness skin models	99:134	OBJECTIVE Full-thickness skin models comprise a three-dimensional dermal equivalent based on an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes.
29130419	12	79	theme	same	1734:1737	arg1	level					1739:1743	the same level	1730:1743	the same level of lipids	1730:1753	The SC contains a similar number of corneocyte layers and the same level of lipids.
29130419	17	80	theme	dermal	2196:2201	arg1	equivalent					2203:2212	a dermal equivalent	2194:2212	a dermal equivalent that mimics the native stromal tissue to a higher extent	2194:2269	The hC-FTMs contain a dermal equivalent that mimics the native stromal tissue to a higher extent.
29130419	9	81	theme	liquid	1365:1370	arg1	spectrometry					1392:1403	liquid chromatography-mass spectrometry	1365:1403	liquid chromatography-mass spectrometry	1365:1403	The stratum corneum (SC) lipid composition was studied with liquid chromatography-mass spectrometry.
29130419	3	82	theme	human	534:538	arg1	collagens					540:548	human collagens	534:548	human collagens	534:548	Animal-derived collagens differ in amino acid composition and physicochemical properties from human collagens.
29130419	5	83	theme	collagen	835:842	arg1	hC-FTMs					867:873	hC-FTMs	867:873	hC-FTMs	867:873	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	5	83	theme	collagen	835:842	arg1	models					859:864	the animal material-free human collagen full-thickness models	804:864	the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue	804:913	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	12	84	theme	corneocyte	1708:1717	arg1	layers					1719:1724	corneocyte layers	1708:1724	corneocyte layers	1708:1724	The SC contains a similar number of corneocyte layers and the same level of lipids.
29130419	11	85	theme	programs	1662:1669	arg1	formation					1581:1589	BM formation	1578:1589	BM formation	1578:1589	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	11	85	theme	programs	1662:1669	arg1	structure					1567:1575	the dermal matrix structure	1549:1575	the dermal matrix structure	1549:1575	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	11	85	theme	programs	1662:1669	arg1	execution					1633:1641	execution	1633:1641	execution of differentiation programs	1633:1669	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	11	85	theme	programs	1662:1669	arg1	proliferation					1614:1626	epidermal basal layer proliferation	1592:1626	epidermal basal layer proliferation	1592:1626	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	5	86	theme	animal	808:813	arg1	hC-FTMs					867:873	hC-FTMs	867:873	hC-FTMs	867:873	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	5	86	theme	animal	808:813	arg1	models					859:864	the animal material-free human collagen full-thickness models	804:864	the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue	804:913	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	7	87	theme	primary	1073:1079	arg1	fibroblasts					1087:1097	primary human fibroblasts	1073:1097	primary human fibroblasts	1073:1097	Both FTMs and hC-FTMs were generated with primary human fibroblasts and keratinocytes.
29130419	1	88	theme	dermal	165:170	arg1	equivalent					172:181	a three-dimensional dermal equivalent	145:181	a three-dimensional dermal equivalent based on an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes	145:301	OBJECTIVE Full-thickness skin models comprise a three-dimensional dermal equivalent based on an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes.
29130419	17	89	theme	stromal	2237:2243	arg1	tissue					2245:2250	the native stromal tissue	2226:2250	the native stromal tissue	2226:2250	The hC-FTMs contain a dermal equivalent that mimics the native stromal tissue to a higher extent.
29130419	4	90	from	formation	668:676	arg1	FTMs					704:707	FTMs	704:707	FTMs	704:707	This composition could alter the functionality of the dermal equivalent and epidermal morphogenesis with the barrier formation in full-thickness models (FTMs).
29130419	4	90	from	formation	668:676	arg1	models					696:701	full-thickness models	681:701	full-thickness models (FTMs)	681:708	This composition could alter the functionality of the dermal equivalent and epidermal morphogenesis with the barrier formation in full-thickness models (FTMs).
29130419	11	91	theme	BM	1578:1579	arg1	formation					1581:1589	BM formation	1578:1589	BM formation	1578:1589	RESULTS The FTMs and hC-FTMs exhibit many similarities, including the dermal matrix structure, BM formation, epidermal basal layer proliferation, and execution of differentiation programs.
29130419	5	92	theme	material-free	815:827	arg1	hC-FTMs					867:873	hC-FTMs	867:873	hC-FTMs	867:873	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	5	92	theme	material-free	815:827	arg1	models					859:864	the animal material-free human collagen full-thickness models	804:864	the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue	804:913	By replacement of animal-derived collagen for human collagen, we generated and characterized the animal material-free human collagen full-thickness models (hC-FTMs) that better mimic native dermal tissue.
29130419	8	93	theme	dermal	1171:1176	arg1	composition					1185:1195	the dermal matrix composition	1167:1195	the dermal matrix composition	1167:1195	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	1	94	link	animal-derived	195:208	arg1	matrix					219:224	an animal-derived collagen matrix	192:224	an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes	192:301	OBJECTIVE Full-thickness skin models comprise a three-dimensional dermal equivalent based on an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes.
29130419	10	95	theme	Lipid	1406:1410	arg1	organization					1421:1432	Lipid lamellar organization	1406:1432	Lipid lamellar organization	1406:1432	Lipid lamellar organization was determined by small-angle X-ray diffraction.
29130419	2	96	theme	cell	429:432	arg1	type					434:437	resident cell type	420:437	resident cell type	420:437	The functionality of both equivalents is influenced by many factors, including extracellular matrix composition and resident cell type.
29130419	18	97	theme	promising	2324:2332	arg1	models					2302:2307	Therefore these in vitro skin models	2272:2307	Therefore these in vitro skin models	2272:2307	Therefore these in vitro skin models can be used as promising tool for research purposes that contribute to animal-free experimentation.
29130419	18	97	theme	promising	2324:2332	arg1	tool					2334:2337	promising tool	2324:2337	promising tool for research purposes that contribute to animal-free experimentation	2324:2406	Therefore these in vitro skin models can be used as promising tool for research purposes that contribute to animal-free experimentation.
29130419	13	98	theme	minor	1837:1841	arg1	differences					1843:1853	only minor differences	1832:1853	only minor differences	1832:1853	The ceramide chain length distribution and ceramide subclass profile showed only minor differences.
29130419	10	99	theme	small-angle	1452:1462	arg1	diffraction					1470:1480	small-angle X-ray diffraction	1452:1480	small-angle X-ray diffraction	1452:1480	Lipid lamellar organization was determined by small-angle X-ray diffraction.
29130419	3	100	theme	amino	475:479	arg1	composition					486:496	amino acid composition	475:496	amino acid composition	475:496	Animal-derived collagens differ in amino acid composition and physicochemical properties from human collagens.
29130419	0	101	theme	Dermal	25:30	arg1	Tissue					32:37	Native Dermal Tissue	18:37	Native Dermal Tissue	18:37	Recapitulation of Native Dermal Tissue in a Full-Thickness Human Skin Model Using Human Collagens.
29130419	8	102	theme	basement	1198:1205	arg1	membrane					1207:1214	basement membrane	1198:1214	basement membrane (BM) formation	1198:1229	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	8	102	theme	basement	1198:1205	arg1	BM					1217:1218	BM	1217:1218	BM	1217:1218	Immunohistochemical analyses with biomarkers for the dermal matrix composition, basement membrane (BM) formation, epidermal proliferation, differentiation, and activation were performed.
29130419	15	103	theme	animal	1933:1938	arg1	hC-FTM					1954:1959	The animal material-free hC-FTM	1929:1959	The animal material-free hC-FTM	1929:1959	CONCLUSION The animal material-free hC-FTM is generated successfully using collagens isolated from human abdominal dermis.
29130419	16	104	theme	dermal	2092:2097	arg1	morphogenesis					2099:2111	(epi-)dermal morphogenesis	2086:2111	(epi-)dermal morphogenesis	2086:2111	Utilization of human collagens revealed that (epi-)dermal morphogenesis and lipid barrier formation resembled that of original FTMs.
29130419	13	105	theme	subclass	1808:1815	arg1	profile					1817:1823	ceramide subclass profile	1799:1823	ceramide subclass profile	1799:1823	The ceramide chain length distribution and ceramide subclass profile showed only minor differences.
29130419	3	106	theme	physicochemical	502:516	arg1	properties					518:527	physicochemical properties	502:527	physicochemical properties	502:527	Animal-derived collagens differ in amino acid composition and physicochemical properties from human collagens.
29130419	0	107	theme	Full-Thickness	44:57	arg1	Model					70:74	a Full-Thickness Human Skin Model	42:74	a Full-Thickness Human Skin Model	42:74	Recapitulation of Native Dermal Tissue in a Full-Thickness Human Skin Model Using Human Collagens.
29130419	15	108	attach	isolated	2003:2010	arg1	dermis					2033:2038	human abdominal dermis	2017:2038	human abdominal dermis	2017:2038	CONCLUSION The animal material-free hC-FTM is generated successfully using collagens isolated from human abdominal dermis.
29130419	15	108	attach	isolated	2003:2010	arg2	collagens					1993:2001	collagens	1993:2001	collagens isolated from human abdominal dermis	1993:2038	CONCLUSION The animal material-free hC-FTM is generated successfully using collagens isolated from human abdominal dermis.
29130419	3	109	theme	Animal-derived	440:453	arg1	collagens					455:463	Animal-derived collagens	440:463	Animal-derived collagens	440:463	Animal-derived collagens differ in amino acid composition and physicochemical properties from human collagens.
29130419	4	110	theme	dermal	605:610	arg1	equivalent					612:621	the dermal equivalent	601:621	the dermal equivalent	601:621	This composition could alter the functionality of the dermal equivalent and epidermal morphogenesis with the barrier formation in full-thickness models (FTMs).
29130419	13	111	theme	ceramide	1760:1767	arg1	distribution					1782:1793	The ceramide chain length distribution	1756:1793	The ceramide chain length distribution	1756:1793	The ceramide chain length distribution and ceramide subclass profile showed only minor differences.
29130419	16	112	theme	barrier	2123:2129	arg1	formation					2131:2139	lipid barrier formation	2117:2139	lipid barrier formation	2117:2139	Utilization of human collagens revealed that (epi-)dermal morphogenesis and lipid barrier formation resembled that of original FTMs.
29130419	3	113	link	Animal-derived	440:453	arg1	collagens					455:463	Animal-derived collagens	440:463	Animal-derived collagens	440:463	Animal-derived collagens differ in amino acid composition and physicochemical properties from human collagens.
29130419	4	114	theme	full-thickness	681:694	arg1	FTMs					704:707	FTMs	704:707	FTMs	704:707	This composition could alter the functionality of the dermal equivalent and epidermal morphogenesis with the barrier formation in full-thickness models (FTMs).
29130419	4	114	theme	full-thickness	681:694	arg1	models					696:701	full-thickness models	681:701	full-thickness models (FTMs)	681:708	This composition could alter the functionality of the dermal equivalent and epidermal morphogenesis with the barrier formation in full-thickness models (FTMs).
29130419	13	115	theme	length	1775:1780	arg1	distribution					1782:1793	The ceramide chain length distribution	1756:1793	The ceramide chain length distribution	1756:1793	The ceramide chain length distribution and ceramide subclass profile showed only minor differences.
29130419	14	116	theme	unaltered	1885:1893	arg1	organization					1904:1915	an unaltered lamellar organization	1882:1915	an unaltered lamellar organization	1882:1915	Subsequently, this led to an unaltered lamellar organization.
29130419	2	117	theme	matrix	397:402	arg1	composition					404:414	extracellular matrix composition	383:414	extracellular matrix composition	383:414	The functionality of both equivalents is influenced by many factors, including extracellular matrix composition and resident cell type.
29130419	1	118	theme	epidermal	258:266	arg1	equivalent					268:277	an epidermal equivalent	255:277	an epidermal equivalent formed by keratinocytes	255:301	OBJECTIVE Full-thickness skin models comprise a three-dimensional dermal equivalent based on an animal-derived collagen matrix that harbors fibroblasts and an epidermal equivalent formed by keratinocytes.
26348386	10	0	theme	surface	1264:1270	arg1	roughness					1272:1280	surface roughness	1264:1280	surface roughness	1264:1280	The conductivity and surface roughness of the coating decayed during the 20-day incubation.
26348386	3	1	theme	electrically	328:339	arg1	scaffolds					352:360	electrically conducting scaffolds	328:360	electrically conducting scaffolds	328:360	Furthermore, electrically conducting scaffolds are required to deliver electrical stimulation to cells.
26348386	8	2	theme	polymerization	1016:1029	arg1	process					1031:1037	the short polymerization process	1006:1037	the short polymerization process we used	1006:1045	Our experiments showed that the molecular weight of CS decreases under oxidizing conditions but that the decay is not significant with the short polymerization process we used.
26348386	9	3	theme	in	1164:1165	arg1	incubation					1173:1182	in vitro incubation	1164:1182	in vitro incubation in phosphate buffer solution at physiological temperature	1164:1240	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	9	4	theme	nonwoven	1087:1094	arg1	fabrics					1100:1106	nonwoven PLA fabrics	1087:1106	nonwoven PLA fabrics	1087:1106	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	9	5	theme	coating	1052:1058	arg1	process					1060:1066	The coating process	1048:1066	The coating process	1048:1066	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	9	6	theme	physiological	1216:1228	arg1	temperature					1230:1240	physiological temperature	1216:1240	physiological temperature	1216:1240	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	4	7	theme	PLA	527:529	arg1	fibers					531:536	biodegradable PLA fibers	513:536	biodegradable PLA fibers	513:536	In this study, uniform, electrically conducting polypyrrole (PPy) coatings were fabricated on biodegradable PLA fibers.
26348386	10	8	dep	conductivity	1247:1258	arg1	The					1243:1245	The	1243:1245	The	1243:1245	The conductivity and surface roughness of the coating decayed during the 20-day incubation.
26348386	2	9	theme	tissue	286:291	arg1	purposes					305:312	tissue engineering purposes	286:312	tissue engineering purposes	286:312	However, the surface properties of PLA scaffolds are not ideal for tissue engineering purposes.
26348386	14	10	theme	Part	1587:1590	arg1	B					1592:1592	Part B	1587:1592	J Biomed Mater Res Part B: Appl Biomater, 104B: 1721-1729, 2016.	1568:1631	J Biomed Mater Res Part B: Appl Biomater, 104B: 1721-1729, 2016.
26348386	11	11	theme	initial	1385:1391	arg1	level					1393:1397	the initial level	1381:1397	the initial level	1381:1397	The mechanical strength, however, remained at the initial level.
26348386	7	12	theme	polymerization	839:852	arg1	degree					829:834	its degree	825:834	its degree of polymerization	825:852	Furthermore, the initial molecular weight of CS and its degree of polymerization were determined.
26348386	7	12	theme	polymerization	839:852	arg1	weight					808:813	the initial molecular weight	786:813	the initial molecular weight of CS	786:819	Furthermore, the initial molecular weight of CS and its degree of polymerization were determined.
26348386	8	13	dep	process	1031:1037	arg1	we					1039:1040	we	1039:1040	we	1039:1040	Our experiments showed that the molecular weight of CS decreases under oxidizing conditions but that the decay is not significant with the short polymerization process we used.
26348386	12	14	theme	fabricated	1410:1419	arg1	suitable					1436:1443	suitable	1436:1443	suitable	1436:1443	Thus, the fabricated structures are suitable for short-term electrical stimulation adequate to promote cell functions in specific cases.
26348386	12	14	theme	fabricated	1410:1419	arg1	structures					1421:1430	the fabricated structures	1406:1430	the fabricated structures	1406:1430	Thus, the fabricated structures are suitable for short-term electrical stimulation adequate to promote cell functions in specific cases.
26348386	6	15	theme	conditions	713:722	arg1	effect					674:679	The effect	670:679	The effect of the oxidative polymerization conditions on the PLA fibers and CS counterion	670:758	The effect of the oxidative polymerization conditions on the PLA fibers and CS counterion was studied.
26348386	8	16	with	significant	989:999	arg1	process					1031:1037	the short polymerization process	1006:1037	the short polymerization process we used	1006:1045	Our experiments showed that the molecular weight of CS decreases under oxidizing conditions but that the decay is not significant with the short polymerization process we used.
26348386	14	17	dep	Res	1583:1585	arg1	1721-1729					1616:1624	1721-1729	1616:1624	1721-1729	1616:1624	J Biomed Mater Res Part B: Appl Biomater, 104B: 1721-1729, 2016.
26348386	14	17	dep	Res	1583:1585	arg1	104B					1610:1613	104B	1610:1613	104B	1610:1613	J Biomed Mater Res Part B: Appl Biomater, 104B: 1721-1729, 2016.
26348386	14	17	dep	Res	1583:1585	arg1	B					1592:1592	Part B	1587:1592	J Biomed Mater Res Part B: Appl Biomater, 104B: 1721-1729, 2016.	1568:1631	J Biomed Mater Res Part B: Appl Biomater, 104B: 1721-1729, 2016.
26348386	14	17	dep	Res	1583:1585	arg1	Biomater					1600:1607	Biomater	1600:1607	Biomater	1600:1607	J Biomed Mater Res Part B: Appl Biomater, 104B: 1721-1729, 2016.
26348386	5	18	theme	chondroitin	583:593	arg1	CS					604:605	CS	604:605	CS	604:605	Biopolymer dopants-hyaluronic acid (HA) and chondroitin sulfate (CS)-were compared, and a PPy/CS composition was analyzed further.
26348386	5	18	theme	chondroitin	583:593	arg1	sulfate					595:601	chondroitin sulfate	583:601	chondroitin sulfate (CS)	583:606	Biopolymer dopants-hyaluronic acid (HA) and chondroitin sulfate (CS)-were compared, and a PPy/CS composition was analyzed further.
26348386	1	19	theme	Three-dimensional	90:106	arg1	scaffolds					117:125	Three-dimensional, fibrous scaffolds	90:125	Three-dimensional, fibrous scaffolds	90:125	Three-dimensional, fibrous scaffolds can be easily fabricated from polylactide (PLA) using melt spinning and textile techniques.
26348386	6	20	theme	polymerization	698:711	arg1	conditions					713:722	the oxidative polymerization conditions	684:722	the oxidative polymerization conditions	684:722	The effect of the oxidative polymerization conditions on the PLA fibers and CS counterion was studied.
26348386	1	21	theme	melt	181:184	arg1	techniques					207:216	melt spinning and textile techniques	181:216	melt spinning and textile techniques	181:216	Three-dimensional, fibrous scaffolds can be easily fabricated from polylactide (PLA) using melt spinning and textile techniques.
26348386	11	22	theme	mechanical	1339:1348	arg1	strength					1350:1357	The mechanical strength	1335:1357	The mechanical strength	1335:1357	The mechanical strength, however, remained at the initial level.
26348386	5	23	theme	PPy/CS	629:634	arg1	composition					636:646	a PPy/CS composition	627:646	a PPy/CS composition	627:646	Biopolymer dopants-hyaluronic acid (HA) and chondroitin sulfate (CS)-were compared, and a PPy/CS composition was analyzed further.
26348386	9	24	theme	buffer	1197:1202	arg1	solution					1204:1211	phosphate buffer solution	1187:1211	phosphate buffer solution	1187:1211	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	6	25	from	effect	674:679	arg1	fibers					735:740	the PLA fibers	727:740	the PLA fibers	727:740	The effect of the oxidative polymerization conditions on the PLA fibers and CS counterion was studied.
26348386	6	25	from	effect	674:679	arg1	counterion					749:758	CS counterion	746:758	CS counterion	746:758	The effect of the oxidative polymerization conditions on the PLA fibers and CS counterion was studied.
26348386	6	26	theme	oxidative	688:696	arg1	conditions					713:722	the oxidative polymerization conditions	684:722	the oxidative polymerization conditions	684:722	The effect of the oxidative polymerization conditions on the PLA fibers and CS counterion was studied.
26348386	1	27	theme	spinning	186:193	arg1	techniques					207:216	melt spinning and textile techniques	181:216	melt spinning and textile techniques	181:216	Three-dimensional, fibrous scaffolds can be easily fabricated from polylactide (PLA) using melt spinning and textile techniques.
26348386	0	28	theme	conductive	25:34	arg1	polypyrrole					53:63	electrically conductive biopolymer-doped polypyrrole	12:63	electrically conductive biopolymer-doped polypyrrole	12:63	Uniform and electrically conductive biopolymer-doped polypyrrole coating for fibrous PLA.
26348386	8	29	theme	short	1010:1014	arg1	process					1031:1037	the short polymerization process	1006:1037	the short polymerization process we used	1006:1045	Our experiments showed that the molecular weight of CS decreases under oxidizing conditions but that the decay is not significant with the short polymerization process we used.
26348386	9	30	theme	PLA	1096:1098	arg1	fabrics					1100:1106	nonwoven PLA fabrics	1087:1106	nonwoven PLA fabrics	1087:1106	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	3	31	theme	electrical	386:395	arg1	stimulation					397:407	electrical stimulation	386:407	electrical stimulation	386:407	Furthermore, electrically conducting scaffolds are required to deliver electrical stimulation to cells.
26348386	10	32	theme	coating	1289:1295	arg1	conductivity					1247:1258	conductivity	1247:1258	conductivity	1247:1258	The conductivity and surface roughness of the coating decayed during the 20-day incubation.
26348386	10	32	theme	coating	1289:1295	arg1	roughness					1272:1280	surface roughness	1264:1280	surface roughness	1264:1280	The conductivity and surface roughness of the coating decayed during the 20-day incubation.
26348386	10	33	theme	20-day	1316:1321	arg1	incubation					1323:1332	the 20-day incubation	1312:1332	the 20-day incubation	1312:1332	The conductivity and surface roughness of the coating decayed during the 20-day incubation.
26348386	1	34	theme	textile	199:205	arg1	techniques					207:216	melt spinning and textile techniques	181:216	melt spinning and textile techniques	181:216	Three-dimensional, fibrous scaffolds can be easily fabricated from polylactide (PLA) using melt spinning and textile techniques.
26348386	8	35	theme	oxidizing	942:950	arg1	conditions					952:961	oxidizing conditions	942:961	oxidizing conditions	942:961	Our experiments showed that the molecular weight of CS decreases under oxidizing conditions but that the decay is not significant with the short polymerization process we used.
26348386	7	36	theme	initial	790:796	arg1	weight					808:813	the initial molecular weight	786:813	the initial molecular weight of CS	786:819	Furthermore, the initial molecular weight of CS and its degree of polymerization were determined.
26348386	4	37	theme	polypyrrole	467:477	arg1	coatings					485:492	uniform, electrically conducting polypyrrole (PPy) coatings	434:492	uniform, electrically conducting polypyrrole (PPy) coatings	434:492	In this study, uniform, electrically conducting polypyrrole (PPy) coatings were fabricated on biodegradable PLA fibers.
26348386	2	38	theme	engineering	293:303	arg1	purposes					305:312	tissue engineering purposes	286:312	tissue engineering purposes	286:312	However, the surface properties of PLA scaffolds are not ideal for tissue engineering purposes.
26348386	7	39	theme	CS	818:819	arg1	degree					829:834	its degree	825:834	its degree of polymerization	825:852	Furthermore, the initial molecular weight of CS and its degree of polymerization were determined.
26348386	7	39	theme	CS	818:819	arg1	weight					808:813	the initial molecular weight	786:813	the initial molecular weight of CS	786:819	Furthermore, the initial molecular weight of CS and its degree of polymerization were determined.
26348386	7	40	theme	molecular	798:806	arg1	weight					808:813	the initial molecular weight	786:813	the initial molecular weight of CS	786:819	Furthermore, the initial molecular weight of CS and its degree of polymerization were determined.
26348386	8	41	theme	molecular	903:911	arg1	weight					913:918	the molecular weight	899:918	the molecular weight of CS	899:924	Our experiments showed that the molecular weight of CS decreases under oxidizing conditions but that the decay is not significant with the short polymerization process we used.
26348386	0	42	theme	biopolymer-doped	36:51	arg1	polypyrrole					53:63	electrically conductive biopolymer-doped polypyrrole	12:63	electrically conductive biopolymer-doped polypyrrole	12:63	Uniform and electrically conductive biopolymer-doped polypyrrole coating for fibrous PLA.
26348386	4	43	theme	PPy	480:482	arg1	coatings					485:492	uniform, electrically conducting polypyrrole (PPy) coatings	434:492	uniform, electrically conducting polypyrrole (PPy) coatings	434:492	In this study, uniform, electrically conducting polypyrrole (PPy) coatings were fabricated on biodegradable PLA fibers.
26348386	2	44	theme	surface	232:238	arg1	properties					240:249	the surface properties	228:249	the surface properties of PLA scaffolds	228:266	However, the surface properties of PLA scaffolds are not ideal for tissue engineering purposes.
26348386	2	44	theme	surface	232:238	arg1	ideal					276:280	ideal	276:280	ideal	276:280	However, the surface properties of PLA scaffolds are not ideal for tissue engineering purposes.
26348386	8	45	theme	CS	923:924	arg1	weight					913:918	the molecular weight	899:918	the molecular weight of CS	899:924	Our experiments showed that the molecular weight of CS decreases under oxidizing conditions but that the decay is not significant with the short polymerization process we used.
26348386	9	46	from	temperature	1230:1240	arg1	incubation					1173:1182	in vitro incubation	1164:1182	in vitro incubation in phosphate buffer solution at physiological temperature	1164:1240	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	12	47	theme	specific	1521:1528	arg1	cases					1530:1534	specific cases	1521:1534	specific cases	1521:1534	Thus, the fabricated structures are suitable for short-term electrical stimulation adequate to promote cell functions in specific cases.
26348386	12	48	theme	electrical	1460:1469	arg1	stimulation					1471:1481	short-term electrical stimulation	1449:1481	short-term electrical stimulation adequate to promote cell functions in specific cases	1449:1534	Thus, the fabricated structures are suitable for short-term electrical stimulation adequate to promote cell functions in specific cases.
26348386	12	49	theme	short-term	1449:1458	arg1	stimulation					1471:1481	short-term electrical stimulation	1449:1481	short-term electrical stimulation adequate to promote cell functions in specific cases	1449:1534	Thus, the fabricated structures are suitable for short-term electrical stimulation adequate to promote cell functions in specific cases.
26348386	4	50	theme	uniform	434:440	arg1	coatings					485:492	uniform, electrically conducting polypyrrole (PPy) coatings	434:492	uniform, electrically conducting polypyrrole (PPy) coatings	434:492	In this study, uniform, electrically conducting polypyrrole (PPy) coatings were fabricated on biodegradable PLA fibers.
26348386	12	51	theme	adequate	1483:1490	arg1	stimulation					1471:1481	short-term electrical stimulation	1449:1481	short-term electrical stimulation adequate to promote cell functions in specific cases	1449:1534	Thus, the fabricated structures are suitable for short-term electrical stimulation adequate to promote cell functions in specific cases.
26348386	1	52	dep	Three-dimensional	90:106	arg1	fibrous					109:115	fibrous	109:115	fibrous	109:115	Three-dimensional, fibrous scaffolds can be easily fabricated from polylactide (PLA) using melt spinning and textile techniques.
26348386	9	53	theme	phosphate	1187:1195	arg1	buffer					1197:1202	phosphate buffer	1187:1202	phosphate buffer solution	1187:1211	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	6	54	theme	CS	746:747	arg1	counterion					749:758	CS counterion	746:758	CS counterion	746:758	The effect of the oxidative polymerization conditions on the PLA fibers and CS counterion was studied.
26348386	6	55	theme	PLA	731:733	arg1	fibers					735:740	the PLA fibers	727:740	the PLA fibers	727:740	The effect of the oxidative polymerization conditions on the PLA fibers and CS counterion was studied.
26348386	9	56	theme	PPy/CS	1130:1135	arg1	coating					1137:1143	PPy/CS coating	1130:1143	PPy/CS coating	1130:1143	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	12	57	theme	cell	1503:1506	arg1	functions					1508:1516	cell functions	1503:1516	cell functions	1503:1516	Thus, the fabricated structures are suitable for short-term electrical stimulation adequate to promote cell functions in specific cases.
26348386	2	58	theme	scaffolds	258:266	arg1	properties					240:249	the surface properties	228:249	the surface properties of PLA scaffolds	228:266	However, the surface properties of PLA scaffolds are not ideal for tissue engineering purposes.
26348386	2	58	theme	scaffolds	258:266	arg1	ideal					276:280	ideal	276:280	ideal	276:280	However, the surface properties of PLA scaffolds are not ideal for tissue engineering purposes.
26348386	9	59	theme	coating	1137:1143	arg1	stability					1117:1125	the stability	1113:1125	the stability of PPy/CS coating	1113:1143	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	9	60	dep	in	1164:1165	arg1	vitro					1167:1171	vitro	1167:1171	vitro	1167:1171	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	3	61	theme	conducting	341:350	arg1	scaffolds					352:360	electrically conducting scaffolds	328:360	electrically conducting scaffolds	328:360	Furthermore, electrically conducting scaffolds are required to deliver electrical stimulation to cells.
26348386	13	62	dep	©	1537:1537	arg1	Inc.					1563:1566	Inc.	1563:1566	Inc.	1563:1566	© 2015 Wiley Periodicals, Inc.
26348386	2	63	theme	PLA	254:256	arg1	scaffolds					258:266	PLA scaffolds	254:266	PLA scaffolds	254:266	However, the surface properties of PLA scaffolds are not ideal for tissue engineering purposes.
26348386	5	64	theme	Biopolymer	539:548	arg1	HA					575:576	HA	575:576	HA	575:576	Biopolymer dopants-hyaluronic acid (HA) and chondroitin sulfate (CS)-were compared, and a PPy/CS composition was analyzed further.
26348386	5	64	theme	Biopolymer	539:548	arg1	acid					569:572	Biopolymer dopants-hyaluronic acid	539:572	Biopolymer dopants-hyaluronic acid (HA)	539:577	Biopolymer dopants-hyaluronic acid (HA) and chondroitin sulfate (CS)-were compared, and a PPy/CS composition was analyzed further.
26348386	9	65	from	incubation	1173:1182	arg1	solution					1204:1211	phosphate buffer solution	1187:1211	phosphate buffer solution	1187:1211	The coating process was transferred to nonwoven PLA fabrics, and the stability of PPy/CS coating was studied during in vitro incubation in phosphate buffer solution at physiological temperature.
26348386	5	66	dep	-were	607:611	arg1	compared					613:620	compared	613:620	-were compared	607:620	Biopolymer dopants-hyaluronic acid (HA) and chondroitin sulfate (CS)-were compared, and a PPy/CS composition was analyzed further.
26348386	4	67	dep	uniform	434:440	arg1	conducting					456:465	conducting	456:465	conducting	456:465	In this study, uniform, electrically conducting polypyrrole (PPy) coatings were fabricated on biodegradable PLA fibers.
26348386	0	68	theme	fibrous	77:83	arg1	PLA					85:87	fibrous PLA	77:87	fibrous PLA	77:87	Uniform and electrically conductive biopolymer-doped polypyrrole coating for fibrous PLA.
26348386	4	69	theme	biodegradable	513:525	arg1	fibers					531:536	biodegradable PLA fibers	513:536	biodegradable PLA fibers	513:536	In this study, uniform, electrically conducting polypyrrole (PPy) coatings were fabricated on biodegradable PLA fibers.
26348386	5	70	theme	dopants-hyaluronic	550:567	arg1	HA					575:576	HA	575:576	HA	575:576	Biopolymer dopants-hyaluronic acid (HA) and chondroitin sulfate (CS)-were compared, and a PPy/CS composition was analyzed further.
26348386	5	70	theme	dopants-hyaluronic	550:567	arg1	acid					569:572	Biopolymer dopants-hyaluronic acid	539:572	Biopolymer dopants-hyaluronic acid (HA)	539:577	Biopolymer dopants-hyaluronic acid (HA) and chondroitin sulfate (CS)-were compared, and a PPy/CS composition was analyzed further.
27217083	8	0	theme	content	1045:1051	arg1	loss					1026:1029	no loss	1023:1029	no loss of amlodipine content	1023:1051	A formulation containing amlodipine besylate, sucrose syrup and methyl paraben remained physically stable for 12months at 4°C with no loss of amlodipine content.
27217083	13	1	theme	commercial	1465:1474	arg1	forms					1483:1487	manipulated commercial dosage forms	1453:1487	manipulated commercial dosage forms	1453:1487	This liquid formulation is preferred over manipulated commercial dosage forms or non-standardized extemporaneously compounded formulations.
27217083	0	2	theme	besylate	61:68	arg1	solution					38:45	an oral solution	30:45	an oral solution of amlodipine besylate	30:68	Design and stability study of an oral solution of amlodipine besylate for pediatric patients.
27217083	8	3	contain	containing	906:915	arg1	formulation					894:904	A formulation	892:904	A formulation containing amlodipine besylate, sucrose syrup and methyl paraben	892:969	A formulation containing amlodipine besylate, sucrose syrup and methyl paraben remained physically stable for 12months at 4°C with no loss of amlodipine content.
27217083	8	3	contain	containing	906:915	arg2	paraben					963:969	methyl paraben	956:969	methyl paraben	956:969	A formulation containing amlodipine besylate, sucrose syrup and methyl paraben remained physically stable for 12months at 4°C with no loss of amlodipine content.
27217083	8	3	contain	containing	906:915	arg2	syrup					946:950	sucrose syrup	938:950	sucrose syrup	938:950	A formulation containing amlodipine besylate, sucrose syrup and methyl paraben remained physically stable for 12months at 4°C with no loss of amlodipine content.
27217083	8	3	contain	containing	906:915	arg2	besylate					928:935	amlodipine besylate	917:935	amlodipine besylate	917:935	A formulation containing amlodipine besylate, sucrose syrup and methyl paraben remained physically stable for 12months at 4°C with no loss of amlodipine content.
27217083	8	4	theme	amlodipine	1034:1043	arg1	content					1045:1051	amlodipine content	1034:1051	amlodipine content	1034:1051	A formulation containing amlodipine besylate, sucrose syrup and methyl paraben remained physically stable for 12months at 4°C with no loss of amlodipine content.
27217083	13	5	theme	dosage	1476:1481	arg1	forms					1483:1487	manipulated commercial dosage forms	1453:1487	manipulated commercial dosage forms	1453:1487	This liquid formulation is preferred over manipulated commercial dosage forms or non-standardized extemporaneously compounded formulations.
27217083	4	6	dep	parameters	520:529	arg1	pH					596:597	pH	596:597	pH	596:597	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	4	6	dep	parameters	520:529	arg1	content					552:558	preservative content	539:558	preservative content	539:558	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	4	6	dep	parameters	520:529	arg1	substances					569:578	related substances	561:578	related substances	561:578	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	4	6	dep	parameters	520:529	arg1	parameters					520:529	The parameters API and preservative content, related substances, appearance and pH	516:597	METHODS The parameters API and preservative content, related substances, appearance and pH	508:597	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	4	6	dep	parameters	520:529	arg1	API					531:533	API	531:533	API	531:533	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	4	6	dep	parameters	520:529	arg1	appearance					581:590	appearance	581:590	appearance	581:590	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	11	7	theme	refrigerated	1193:1204	arg1	conditions					1206:1215	refrigerated conditions	1193:1215	refrigerated conditions	1193:1215	DISCUSSION Storage under refrigerated conditions was necessary to prevent precipitation and to obtain an acceptable shelf-life.
27217083	1	8	theme	INTRODUCTION	94:105	arg1	Amlodipine					107:116	INTRODUCTION Amlodipine	94:116	INTRODUCTION Amlodipine	94:116	INTRODUCTION Amlodipine is an antihypertensive agent recommended for the management of hypertension in children and adolescents.
27217083	1	8	theme	INTRODUCTION	94:105	arg1	agent					141:145	an antihypertensive agent	121:145	an antihypertensive agent recommended for the management of hypertension in children and adolescents	121:220	INTRODUCTION Amlodipine is an antihypertensive agent recommended for the management of hypertension in children and adolescents.
27217083	0	9	theme	pediatric	74:82	arg1	patients					84:91	pediatric patients	74:91	pediatric patients	74:91	Design and stability study of an oral solution of amlodipine besylate for pediatric patients.
27217083	10	10	theme	In-use	1125:1130	arg1	stability					1132:1140	In-use stability	1125:1140	In-use stability	1125:1140	In-use stability was proven up to 18weeks.
27217083	5	11	dep	12months	681:688	arg1	up					675:676	up	675:676	up	675:676	Samples were analyzed up to 12months.
27217083	3	12	theme	robust	426:431	arg1	process					447:453	a robust manufacturing process	424:453	a robust manufacturing process suitable for ex-tempora and larger scale production	424:505	Our goal was to develop a pediatric oral solution of amlodipine, using a robust manufacturing process suitable for ex-tempora and larger scale production.
27217083	13	13	theme	manipulated	1453:1463	arg1	forms					1483:1487	manipulated commercial dosage forms	1453:1487	manipulated commercial dosage forms	1453:1487	This liquid formulation is preferred over manipulated commercial dosage forms or non-standardized extemporaneously compounded formulations.
27217083	7	14	theme	storage	856:862	arg1	conditions					864:873	storage conditions	856:873	storage conditions	856:873	RESULTS The stability of the formulation was influenced by storage conditions and composition.
27217083	1	15	theme	hypertension	181:192	arg1	management					167:176	the management	163:176	the management of hypertension in children and adolescents	163:220	INTRODUCTION Amlodipine is an antihypertensive agent recommended for the management of hypertension in children and adolescents.
27217083	3	16	theme	pediatric	379:387	arg1	solution					394:401	a pediatric oral solution	377:401	a pediatric oral solution of amlodipine	377:415	Our goal was to develop a pediatric oral solution of amlodipine, using a robust manufacturing process suitable for ex-tempora and larger scale production.
27217083	13	17	theme	liquid	1416:1421	arg1	formulation					1423:1433	This liquid formulation	1411:1433	This liquid formulation	1411:1433	This liquid formulation is preferred over manipulated commercial dosage forms or non-standardized extemporaneously compounded formulations.
27217083	6	18	theme	dosing	780:785	arg1	schedule					787:794	a two-times daily dosing schedule	762:794	a two-times daily dosing schedule	762:794	Microbiological quality was studied in an 18-week in-use test based on a two-times daily dosing schedule.
27217083	12	19	theme	pediatric	1389:1397	arg1	population					1399:1408	the pediatric population	1385:1408	the pediatric population	1385:1408	In conclusion, we have developed and validated an amlodipine oral solution, suitable for the pediatric population.
27217083	6	20	theme	Microbiological	691:705	arg1	quality					707:713	Microbiological quality	691:713	Microbiological quality	691:713	Microbiological quality was studied in an 18-week in-use test based on a two-times daily dosing schedule.
27217083	13	21	theme	compounded	1526:1535	arg1	formulations					1537:1548	non-standardized extemporaneously compounded formulations	1492:1548	non-standardized extemporaneously compounded formulations	1492:1548	This liquid formulation is preferred over manipulated commercial dosage forms or non-standardized extemporaneously compounded formulations.
27217083	6	22	theme	daily	774:778	arg1	schedule					787:794	a two-times daily dosing schedule	762:794	a two-times daily dosing schedule	762:794	Microbiological quality was studied in an 18-week in-use test based on a two-times daily dosing schedule.
27217083	3	23	theme	suitable	455:462	arg1	process					447:453	a robust manufacturing process	424:453	a robust manufacturing process suitable for ex-tempora and larger scale production	424:505	Our goal was to develop a pediatric oral solution of amlodipine, using a robust manufacturing process suitable for ex-tempora and larger scale production.
27217083	6	24	theme	in-use	741:746	arg1	test					748:751	an 18-week in-use test	730:751	an 18-week in-use test based on a two-times daily dosing schedule	730:794	Microbiological quality was studied in an 18-week in-use test based on a two-times daily dosing schedule.
27217083	2	25	theme	necessary	291:299	arg1	flexibility					301:311	the necessary flexibility	287:311	the necessary flexibility in dosing needed for treating children	287:350	The commercially available tablets of 5 and 10mg do not provide the necessary flexibility in dosing needed for treating children.
27217083	4	26	theme	different	623:631	arg1	conditions					641:650	four different storage conditions	618:650	four different storage conditions	618:650	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	4	27	theme	related	561:567	arg1	substances					569:578	related substances	561:578	related substances	561:578	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	12	28	theme	suitable	1372:1379	arg1	solution					1362:1369	an amlodipine oral solution	1343:1369	an amlodipine oral solution	1343:1369	In conclusion, we have developed and validated an amlodipine oral solution, suitable for the pediatric population.
27217083	7	29	theme	formulation	826:836	arg1	stability					809:817	The stability	805:817	The stability of the formulation	805:836	RESULTS The stability of the formulation was influenced by storage conditions and composition.
27217083	3	30	theme	manufacturing	433:445	arg1	process					447:453	a robust manufacturing process	424:453	a robust manufacturing process suitable for ex-tempora and larger scale production	424:505	Our goal was to develop a pediatric oral solution of amlodipine, using a robust manufacturing process suitable for ex-tempora and larger scale production.
27217083	3	31	theme	amlodipine	406:415	arg1	solution					394:401	a pediatric oral solution	377:401	a pediatric oral solution of amlodipine	377:415	Our goal was to develop a pediatric oral solution of amlodipine, using a robust manufacturing process suitable for ex-tempora and larger scale production.
27217083	2	32	theme	available	240:248	arg1	tablets					250:256	The commercially available tablets	223:256	The commercially available tablets of 5 and 10mg	223:270	The commercially available tablets of 5 and 10mg do not provide the necessary flexibility in dosing needed for treating children.
27217083	6	33	theme	two-times	764:772	arg1	schedule					787:794	a two-times daily dosing schedule	762:794	a two-times daily dosing schedule	762:794	Microbiological quality was studied in an 18-week in-use test based on a two-times daily dosing schedule.
27217083	0	34	theme	solution	38:45	arg1	stability					11:19	stability	11:19	stability	11:19	Design and stability study of an oral solution of amlodipine besylate for pediatric patients.
27217083	0	34	theme	solution	38:45	arg1	Design					0:5	Design	0:5	Design	0:5	Design and stability study of an oral solution of amlodipine besylate for pediatric patients.
27217083	4	35	dep	METHODS	508:514	arg1	parameters					520:529	The parameters API and preservative content, related substances, appearance and pH	516:597	METHODS The parameters API and preservative content, related substances, appearance and pH	508:597	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	4	35	dep	METHODS	508:514	arg1	pH					596:597	pH	596:597	pH	596:597	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	4	35	dep	METHODS	508:514	arg1	API					531:533	API	531:533	API	531:533	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	4	35	dep	METHODS	508:514	arg1	content					552:558	preservative content	539:558	preservative content	539:558	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	4	35	dep	METHODS	508:514	arg1	appearance					581:590	appearance	581:590	appearance	581:590	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	8	36	theme	sucrose	938:944	arg1	syrup					946:950	sucrose syrup	938:950	sucrose syrup	938:950	A formulation containing amlodipine besylate, sucrose syrup and methyl paraben remained physically stable for 12months at 4°C with no loss of amlodipine content.
27217083	3	37	theme	larger	483:488	arg1	production					496:505	larger scale production	483:505	larger scale production	483:505	Our goal was to develop a pediatric oral solution of amlodipine, using a robust manufacturing process suitable for ex-tempora and larger scale production.
27217083	0	38	theme	oral	33:36	arg1	solution					38:45	an oral solution	30:45	an oral solution of amlodipine besylate	30:68	Design and stability study of an oral solution of amlodipine besylate for pediatric patients.
27217083	4	39	theme	preservative	539:550	arg1	parameters					520:529	The parameters API and preservative content, related substances, appearance and pH	516:597	METHODS The parameters API and preservative content, related substances, appearance and pH	508:597	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	4	39	theme	preservative	539:550	arg1	content					552:558	preservative content	539:558	preservative content	539:558	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	10	40	dep	18weeks	1159:1165	arg1	up					1153:1154	up	1153:1154	up	1153:1154	In-use stability was proven up to 18weeks.
27217083	8	41	theme	amlodipine	917:926	arg1	besylate					928:935	amlodipine besylate	917:935	amlodipine besylate	917:935	A formulation containing amlodipine besylate, sucrose syrup and methyl paraben remained physically stable for 12months at 4°C with no loss of amlodipine content.
27217083	8	42	theme	methyl	956:961	arg1	paraben					963:969	methyl paraben	956:969	methyl paraben	956:969	A formulation containing amlodipine besylate, sucrose syrup and methyl paraben remained physically stable for 12months at 4°C with no loss of amlodipine content.
27217083	3	43	theme	scale	490:494	arg1	production					496:505	larger scale production	483:505	larger scale production	483:505	Our goal was to develop a pediatric oral solution of amlodipine, using a robust manufacturing process suitable for ex-tempora and larger scale production.
27217083	12	44	theme	oral	1357:1360	arg1	solution					1362:1369	an amlodipine oral solution	1343:1369	an amlodipine oral solution	1343:1369	In conclusion, we have developed and validated an amlodipine oral solution, suitable for the pediatric population.
27217083	12	45	theme	amlodipine	1346:1355	arg1	solution					1362:1369	an amlodipine oral solution	1343:1369	an amlodipine oral solution	1343:1369	In conclusion, we have developed and validated an amlodipine oral solution, suitable for the pediatric population.
27217083	2	46	theme	10mg	267:270	arg1	tablets					250:256	The commercially available tablets	223:256	The commercially available tablets of 5 and 10mg	223:270	The commercially available tablets of 5 and 10mg do not provide the necessary flexibility in dosing needed for treating children.
27217083	13	47	theme	non-standardized	1492:1507	arg1	formulations					1537:1548	non-standardized extemporaneously compounded formulations	1492:1548	non-standardized extemporaneously compounded formulations	1492:1548	This liquid formulation is preferred over manipulated commercial dosage forms or non-standardized extemporaneously compounded formulations.
27217083	6	48	theme	18-week	733:739	arg1	test					748:751	an 18-week in-use test	730:751	an 18-week in-use test based on a two-times daily dosing schedule	730:794	Microbiological quality was studied in an 18-week in-use test based on a two-times daily dosing schedule.
27217083	11	49	theme	acceptable	1273:1282	arg1	shelf-life					1284:1293	an acceptable shelf-life	1270:1293	an acceptable shelf-life	1270:1293	DISCUSSION Storage under refrigerated conditions was necessary to prevent precipitation and to obtain an acceptable shelf-life.
27217083	11	50	theme	DISCUSSION	1168:1177	arg1	Storage					1179:1185	DISCUSSION Storage	1168:1185	DISCUSSION Storage under refrigerated conditions	1168:1215	DISCUSSION Storage under refrigerated conditions was necessary to prevent precipitation and to obtain an acceptable shelf-life.
27217083	1	51	theme	antihypertensive	124:139	arg1	Amlodipine					107:116	INTRODUCTION Amlodipine	94:116	INTRODUCTION Amlodipine	94:116	INTRODUCTION Amlodipine is an antihypertensive agent recommended for the management of hypertension in children and adolescents.
27217083	1	51	theme	antihypertensive	124:139	arg1	agent					141:145	an antihypertensive agent	121:145	an antihypertensive agent recommended for the management of hypertension in children and adolescents	121:220	INTRODUCTION Amlodipine is an antihypertensive agent recommended for the management of hypertension in children and adolescents.
27217083	0	52	theme	amlodipine	50:59	arg1	besylate					61:68	amlodipine besylate	50:68	amlodipine besylate	50:68	Design and stability study of an oral solution of amlodipine besylate for pediatric patients.
27217083	2	53	theme	5	261:261	arg1	tablets					250:256	The commercially available tablets	223:256	The commercially available tablets of 5 and 10mg	223:270	The commercially available tablets of 5 and 10mg do not provide the necessary flexibility in dosing needed for treating children.
27217083	4	54	theme	storage	633:639	arg1	conditions					641:650	four different storage conditions	618:650	four different storage conditions	618:650	METHODS The parameters API and preservative content, related substances, appearance and pH were studied under four different storage conditions.
27217083	9	55	theme	Related	1054:1060	arg1	substances					1062:1071	Related substances	1054:1071	Related substances	1054:1071	Related substances increased during the study but remained below 0.5%.
27217083	3	56	theme	oral	389:392	arg1	solution					394:401	a pediatric oral solution	377:401	a pediatric oral solution of amlodipine	377:415	Our goal was to develop a pediatric oral solution of amlodipine, using a robust manufacturing process suitable for ex-tempora and larger scale production.
27217083	7	57	dep	RESULTS	797:803	arg1	influenced					842:851	influenced	842:851	was influenced by storage conditions and composition	838:889	RESULTS The stability of the formulation was influenced by storage conditions and composition.
27217083	0	58	dep	Design	0:5	arg1	study					21:25	study	21:25	study	21:25	Design and stability study of an oral solution of amlodipine besylate for pediatric patients.
27217083	1	59	from	management	167:176	arg1	adolescents					210:220	adolescents	210:220	adolescents	210:220	INTRODUCTION Amlodipine is an antihypertensive agent recommended for the management of hypertension in children and adolescents.
27217083	1	59	from	management	167:176	arg1	children					197:204	children	197:204	children	197:204	INTRODUCTION Amlodipine is an antihypertensive agent recommended for the management of hypertension in children and adolescents.
27217083	11	60	dep	necessary	1221:1229	arg1	obtain					1263:1268	obtain	1263:1268	to obtain an acceptable shelf-life	1260:1293	DISCUSSION Storage under refrigerated conditions was necessary to prevent precipitation and to obtain an acceptable shelf-life.
27217083	11	60	dep	necessary	1221:1229	arg1	prevent					1234:1240	prevent	1234:1240	to prevent precipitation	1231:1254	DISCUSSION Storage under refrigerated conditions was necessary to prevent precipitation and to obtain an acceptable shelf-life.
27217083	2	61	from	flexibility	301:311	arg1	dosing					316:321	dosing	316:321	dosing	316:321	The commercially available tablets of 5 and 10mg do not provide the necessary flexibility in dosing needed for treating children.
29168768	6	0	theme	composite	919:927	arg1	films					929:933	organic-inorganic composite films	901:933	organic-inorganic composite films	901:933	Possible applications of the cinnamic anhydride-acylated xylan derivatives include wet-end papermaking, organic-inorganic composite films, and hydrogels.
29168768	6	1	theme	cinnamic	826:833	arg1	derivatives					860:870	the cinnamic anhydride-acylated xylan derivatives	822:870	the cinnamic anhydride-acylated xylan derivatives	822:870	Possible applications of the cinnamic anhydride-acylated xylan derivatives include wet-end papermaking, organic-inorganic composite films, and hydrogels.
29168768	3	2	theme	cinnamic	489:496	arg1	ratio					520:524	cinnamic anhydride/xylan molar ratio	489:524	cinnamic anhydride/xylan molar ratio	489:524	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	4	3	theme	derivatives	583:593	arg1	stability					566:574	the thermal stability	554:574	the thermal stability	554:574	The chemical structure and the thermal stability of the derivatives were characterized by Fourier transform infrared spectroscopy (FT-IR), 13C-NMR spectroscopy, and thermogravimetry.
29168768	4	3	theme	derivatives	583:593	arg1	structure					540:548	The chemical structure	527:548	The chemical structure	527:548	The chemical structure and the thermal stability of the derivatives were characterized by Fourier transform infrared spectroscopy (FT-IR), 13C-NMR spectroscopy, and thermogravimetry.
29168768	3	4	theme	reaction	463:470	arg1	temperature					472:482	reaction temperature	463:482	reaction temperature	463:482	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	5	5	theme	derivatives	739:749	arg1	stability					722:730	The thermal stability	710:730	The thermal stability of the derivatives	710:749	The thermal stability of the derivatives was reduced compared with the original xylan.
29168768	1	6	theme	anhydride	192:200	arg1	grafting					171:178	an ionic liquid-mediated homogeneous grafting	134:178	an ionic liquid-mediated homogeneous grafting of cinnamic anhydride to xylans	134:210	A new functional biopolymer was synthesized through an ionic liquid-mediated homogeneous grafting of cinnamic anhydride to xylans.
29168768	5	7	theme	original	781:788	arg1	xylan					790:794	the original xylan	777:794	the original xylan	777:794	The thermal stability of the derivatives was reduced compared with the original xylan.
29168768	6	8	theme	wet-end	880:886	arg1	papermaking					888:898	wet-end papermaking	880:898	wet-end papermaking	880:898	Possible applications of the cinnamic anhydride-acylated xylan derivatives include wet-end papermaking, organic-inorganic composite films, and hydrogels.
29168768	1	9	theme	new	84:86	arg1	biopolymer					99:108	A new functional biopolymer	82:108	A new functional biopolymer	82:108	A new functional biopolymer was synthesized through an ionic liquid-mediated homogeneous grafting of cinnamic anhydride to xylans.
29168768	0	10	theme	Liquid-Mediated	6:20	arg1	Esterification					34:47	Liquid-Mediated Homogeneous Esterification	6:47	Liquid-Mediated Homogeneous Esterification of Cinnamic Anhydride to Xylans	6:79	Ionic Liquid-Mediated Homogeneous Esterification of Cinnamic Anhydride to Xylans.
29168768	3	11	from	system	406:411	arg1	accessible					367:376	accessible	367:376	accessible	367:376	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	3	12	dep	catalysts	425:433	arg1	KOH					442:444	KOH	442:444	KOH	442:444	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	3	12	dep	catalysts	425:433	arg1	NaOH					436:439	NaOH	436:439	NaOH	436:439	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	3	12	dep	catalysts	425:433	arg1	catalysts					425:433	catalysts	425:433	catalysts (NaOH, KOH and LiOH)	425:454	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	3	12	dep	catalysts	425:433	arg1	LiOH					450:453	LiOH	450:453	LiOH	450:453	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	1	13	theme	functional	88:97	arg1	biopolymer					99:108	A new functional biopolymer	82:108	A new functional biopolymer	82:108	A new functional biopolymer was synthesized through an ionic liquid-mediated homogeneous grafting of cinnamic anhydride to xylans.
29168768	4	14	theme	13C-NMR	666:672	arg1	spectroscopy					674:685	13C-NMR spectroscopy	666:685	13C-NMR spectroscopy	666:685	The chemical structure and the thermal stability of the derivatives were characterized by Fourier transform infrared spectroscopy (FT-IR), 13C-NMR spectroscopy, and thermogravimetry.
29168768	6	15	theme	Possible	797:804	arg1	applications					806:817	Possible applications	797:817	Possible applications of the cinnamic anhydride-acylated xylan derivatives	797:870	Possible applications of the cinnamic anhydride-acylated xylan derivatives include wet-end papermaking, organic-inorganic composite films, and hydrogels.
29168768	2	16	dep	ionic	285:289	arg1	ionic					285:289	ionic	285:289	ionic	285:289	The ionic liquid used was 1-allyl-3-methylimidazolium chloride (AMIMCl) ionic liquid.
29168768	2	16	dep	ionic	285:289	arg1	liquid					223:228	liquid	223:228	liquid	223:228	The ionic liquid used was 1-allyl-3-methylimidazolium chloride (AMIMCl) ionic liquid.
29168768	2	16	dep	ionic	285:289	arg1	liquid					291:296	liquid	291:296	liquid	291:296	The ionic liquid used was 1-allyl-3-methylimidazolium chloride (AMIMCl) ionic liquid.
29168768	3	17	theme	molar	514:518	arg1	ratio					520:524	cinnamic anhydride/xylan molar ratio	489:524	cinnamic anhydride/xylan molar ratio	489:524	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	4	18	theme	thermal	558:564	arg1	stability					566:574	the thermal stability	554:574	the thermal stability	554:574	The chemical structure and the thermal stability of the derivatives were characterized by Fourier transform infrared spectroscopy (FT-IR), 13C-NMR spectroscopy, and thermogravimetry.
29168768	6	19	theme	organic-inorganic	901:917	arg1	films					929:933	organic-inorganic composite films	901:933	organic-inorganic composite films	901:933	Possible applications of the cinnamic anhydride-acylated xylan derivatives include wet-end papermaking, organic-inorganic composite films, and hydrogels.
29168768	1	20	theme	cinnamic	183:190	arg1	anhydride					192:200	cinnamic anhydride	183:200	cinnamic anhydride to xylans	183:210	A new functional biopolymer was synthesized through an ionic liquid-mediated homogeneous grafting of cinnamic anhydride to xylans.
29168768	3	21	with	Xylans	299:304	arg1	degrees					311:317	degrees	311:317	degrees of substitution (DS)	311:338	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	0	22	theme	Cinnamic	52:59	arg1	Anhydride					61:69	Cinnamic Anhydride	52:69	Cinnamic Anhydride to Xylans	52:79	Ionic Liquid-Mediated Homogeneous Esterification of Cinnamic Anhydride to Xylans.
29168768	5	23	theme	thermal	714:720	arg1	stability					722:730	The thermal stability	710:730	The thermal stability of the derivatives	710:749	The thermal stability of the derivatives was reduced compared with the original xylan.
29168768	3	24	from	accessible	367:376	arg1	system					406:411	a completely homogeneous system	381:411	a completely homogeneous system	381:411	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	6	25	theme	derivatives	860:870	arg1	applications					806:817	Possible applications	797:817	Possible applications of the cinnamic anhydride-acylated xylan derivatives	797:870	Possible applications of the cinnamic anhydride-acylated xylan derivatives include wet-end papermaking, organic-inorganic composite films, and hydrogels.
29168768	0	26	theme	Anhydride	61:69	arg1	Esterification					34:47	Liquid-Mediated Homogeneous Esterification	6:47	Liquid-Mediated Homogeneous Esterification of Cinnamic Anhydride to Xylans	6:79	Ionic Liquid-Mediated Homogeneous Esterification of Cinnamic Anhydride to Xylans.
29168768	4	27	theme	chemical	531:538	arg1	structure					540:548	The chemical structure	527:548	The chemical structure	527:548	The chemical structure and the thermal stability of the derivatives were characterized by Fourier transform infrared spectroscopy (FT-IR), 13C-NMR spectroscopy, and thermogravimetry.
29168768	4	28	dep	Fourier	617:623	arg1	transform					625:633	transform	625:633	transform infrared spectroscopy (FT-IR), 13C-NMR spectroscopy, and thermogravimetry	625:707	The chemical structure and the thermal stability of the derivatives were characterized by Fourier transform infrared spectroscopy (FT-IR), 13C-NMR spectroscopy, and thermogravimetry.
29168768	4	29	dep	transform	625:633	arg1	infrared					635:642	infrared	635:642	transform infrared spectroscopy (FT-IR), 13C-NMR spectroscopy, and thermogravimetry	625:707	The chemical structure and the thermal stability of the derivatives were characterized by Fourier transform infrared spectroscopy (FT-IR), 13C-NMR spectroscopy, and thermogravimetry.
29168768	1	30	theme	homogeneous	159:169	arg1	grafting					171:178	an ionic liquid-mediated homogeneous grafting	134:178	an ionic liquid-mediated homogeneous grafting of cinnamic anhydride to xylans	134:210	A new functional biopolymer was synthesized through an ionic liquid-mediated homogeneous grafting of cinnamic anhydride to xylans.
29168768	1	31	theme	ionic	137:141	arg1	grafting					171:178	an ionic liquid-mediated homogeneous grafting	134:178	an ionic liquid-mediated homogeneous grafting of cinnamic anhydride to xylans	134:210	A new functional biopolymer was synthesized through an ionic liquid-mediated homogeneous grafting of cinnamic anhydride to xylans.
29168768	3	32	theme	substitution	322:333	arg1	degrees					311:317	degrees	311:317	degrees of substitution (DS)	311:338	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	6	33	theme	xylan	854:858	arg1	derivatives					860:870	the cinnamic anhydride-acylated xylan derivatives	822:870	the cinnamic anhydride-acylated xylan derivatives	822:870	Possible applications of the cinnamic anhydride-acylated xylan derivatives include wet-end papermaking, organic-inorganic composite films, and hydrogels.
29168768	1	34	theme	liquid-mediated	143:157	arg1	grafting					171:178	an ionic liquid-mediated homogeneous grafting	134:178	an ionic liquid-mediated homogeneous grafting of cinnamic anhydride to xylans	134:210	A new functional biopolymer was synthesized through an ionic liquid-mediated homogeneous grafting of cinnamic anhydride to xylans.
29168768	6	35	theme	anhydride-acylated	835:852	arg1	derivatives					860:870	the cinnamic anhydride-acylated xylan derivatives	822:870	the cinnamic anhydride-acylated xylan derivatives	822:870	Possible applications of the cinnamic anhydride-acylated xylan derivatives include wet-end papermaking, organic-inorganic composite films, and hydrogels.
29168768	3	36	theme	anhydride/xylan	498:512	arg1	ratio					520:524	cinnamic anhydride/xylan molar ratio	489:524	cinnamic anhydride/xylan molar ratio	489:524	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
29168768	0	37	theme	Homogeneous	22:32	arg1	Esterification					34:47	Liquid-Mediated Homogeneous Esterification	6:47	Liquid-Mediated Homogeneous Esterification of Cinnamic Anhydride to Xylans	6:79	Ionic Liquid-Mediated Homogeneous Esterification of Cinnamic Anhydride to Xylans.
29168768	3	38	theme	homogeneous	394:404	arg1	system					406:411	a completely homogeneous system	381:411	a completely homogeneous system	381:411	Xylans with degrees of substitution (DS) between 0.11 and 0.57 were accessible in a completely homogeneous system by changing catalysts (NaOH, KOH and LiOH), time, reaction temperature, and cinnamic anhydride/xylan molar ratio.
26043371	3	0	theme	%	867:867	arg1	rejection					850:858	an oil rejection	843:858	an oil rejection of 99.9%	843:867	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	8	1	theme	low	1652:1654	arg1	behaviors					1664:1672	low fouling behaviors	1652:1672	low fouling behaviors	1652:1672	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	6	2	theme	outer	1268:1272	arg1	surface					1274:1280	the fiber outer surface	1258:1280	the fiber outer surface	1258:1280	The water flux of the fouled membrane can be effectively restored to 97% of the original value by water rinses on the fiber outer surface without using any chemicals.
26043371	2	3	dep	salt	686:689	arg1	rejection					699:707	rejection	699:707	rejection	699:707	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	3	4	theme	soybean	886:892	arg1	emulsion					904:911	a 2000 ppm soybean oil/water emulsion	875:911	a 2000 ppm soybean oil/water emulsion	875:911	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	3	4	theme	soybean	886:892	arg1	NaCl					933:936	the feed and 1 M NaCl	916:936	the feed and 1 M NaCl as the draw solution	916:957	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	8	5	theme	oily	1678:1681	arg1	wastewater					1683:1692	oily wastewater	1678:1692	oily wastewater	1678:1692	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	8	6	theme	purification	1794:1805	arg1	purposes					1807:1814	water purification purposes	1788:1814	water purification purposes	1788:1814	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	2	7	theme	propensity	726:735	arg1	characteristics					638:652	characteristics	638:652	characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity	638:735	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	6	8	theme	fouled	1166:1171	arg1	membrane					1173:1180	the fouled membrane	1162:1180	the fouled membrane	1162:1180	The water flux of the fouled membrane can be effectively restored to 97% of the original value by water rinses on the fiber outer surface without using any chemicals.
26043371	6	9	from	rinses	1248:1253	arg1	surface					1274:1280	the fiber outer surface	1258:1280	the fiber outer surface	1258:1280	The water flux of the fouled membrane can be effectively restored to 97% of the original value by water rinses on the fiber outer surface without using any chemicals.
26043371	7	10	theme	emulsion	1419:1426	arg1	recovery					1378:1385	the water recovery	1368:1385	the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl	1368:1493	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	2	11	theme	low	714:716	arg1	propensity					726:735	low fouling propensity	714:735	low fouling propensity	714:735	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	1	12	theme	membrane	192:199	arg1	material					201:208	the membrane material	188:208	the membrane material for the hollow fiber substrate	188:239	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	12	theme	membrane	192:199	arg1	butyrate					170:177	a novel hydrophilic cellulose acetate butyrate	132:177	a novel hydrophilic cellulose acetate butyrate (CAB)	132:183	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	7	13	theme	soybean	1401:1407	arg1	emulsion					1419:1426	a 2000 ppm soybean oil/water emulsion	1390:1426	a 2000 ppm soybean oil/water emulsion	1390:1426	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	5	14	theme	water	1043:1047	arg1	%					1072:1072	only 10%	1065:1072	only 10% of the initial value	1065:1093	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	5	14	theme	water	1043:1047	arg1	value					1089:1093	the initial value	1077:1093	the initial value	1077:1093	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	5	14	theme	water	1043:1047	arg1	decline					1054:1060	the water flux decline	1039:1060	the water flux decline	1039:1060	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	2	15	theme	water	662:666	arg1	flux					668:671	high water flux	657:671	high water flux	657:671	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	6	16	theme	water	1148:1152	arg1	flux					1154:1157	The water flux	1144:1157	The water flux of the fouled membrane	1144:1180	The water flux of the fouled membrane can be effectively restored to 97% of the original value by water rinses on the fiber outer surface without using any chemicals.
26043371	8	17	theme	insightful	1554:1563	arg1	guidelines					1565:1574	insightful guidelines	1554:1574	insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors	1554:1672	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	1	18	theme	osmosis	563:569	arg1	mode					577:580	osmosis (PRO) mode	563:580	osmosis (PRO) mode	563:580	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	3	19	theme	PRO	748:750	arg1	mode					752:755	the PRO mode	744:755	the PRO mode	744:755	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	8	20	theme	alternative	1729:1739	arg1	perspective					1741:1751	an alternative perspective	1726:1751	an alternative perspective	1726:1751	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	1	21	theme	composite	392:400	arg1	membranes					408:416	the thin-film composite (TFC) membranes	378:416	the thin-film composite (TFC) membranes	378:416	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	21	theme	composite	392:400	arg1	TFC					403:405	TFC	403:405	TFC	403:405	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	3	22	theme	TFC-FO	778:783	arg1	membrane					785:792	the newly developed TFC-FO membrane	758:792	the newly developed TFC-FO membrane	758:792	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	2	23	theme	salt	686:689	arg1	characteristics					638:652	characteristics	638:652	characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity	638:735	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	1	24	theme	PRO	572:574	arg1	mode					577:580	osmosis (PRO) mode	563:580	osmosis (PRO) mode	563:580	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	0	25	theme	forward	64:70	arg1	membranes					93:101	effective forward osmosis hollow fiber membranes	54:101	effective forward osmosis hollow fiber membranes	54:101	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.
26043371	1	26	theme	novel	134:138	arg1	material					201:208	the membrane material	188:208	the membrane material for the hollow fiber substrate	188:239	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	26	theme	novel	134:138	arg1	CAB					180:182	CAB	180:182	CAB	180:182	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	26	theme	novel	134:138	arg1	butyrate					170:177	a novel hydrophilic cellulose acetate butyrate	132:177	a novel hydrophilic cellulose acetate butyrate (CAB)	132:183	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	0	27	theme	hollow	80:85	arg1	membranes					93:101	effective forward osmosis hollow fiber membranes	54:101	effective forward osmosis hollow fiber membranes	54:101	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.
26043371	1	28	theme	inner	307:311	arg1	surface					313:319	inner surface	307:319	inner surface	307:319	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	8	29	theme	effective	1599:1607	arg1	membranes					1616:1624	effective TFC-FO membranes	1599:1624	effective TFC-FO membranes	1599:1624	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	1	30	theme	cellulose	152:160	arg1	material					201:208	the membrane material	188:208	the membrane material for the hollow fiber substrate	188:239	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	30	theme	cellulose	152:160	arg1	CAB					180:182	CAB	180:182	CAB	180:182	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	30	theme	cellulose	152:160	arg1	butyrate					170:177	a novel hydrophilic cellulose acetate butyrate	132:177	a novel hydrophilic cellulose acetate butyrate (CAB)	132:183	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	3	31	theme	water	805:809	arg1	flux					811:814	a water flux	803:814	a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution	803:957	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	2	32	theme	TFC-FO	603:608	arg1	membrane					623:630	The newly developed TFC-FO hollow fiber membrane	583:630	The newly developed TFC-FO hollow fiber membrane	583:630	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	5	33	theme	12 h	1108:1111	arg1	test					1113:1116	a 12 h test	1106:1116	a 12 h test for oil/water separation	1106:1141	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	8	34	theme	new	1770:1772	arg1	materials					1774:1782	new materials	1770:1782	new materials for water purification purposes	1770:1814	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	8	35	theme	membranes	1616:1624	arg1	fabrication					1584:1594	the fabrication	1580:1594	the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors	1580:1672	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	7	36	dep	flux	1328:1331	arg1	declines					1333:1340	declines	1333:1340	declines	1333:1340	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	8	37	theme	high	1631:1634	arg1	performance					1636:1646	high performance	1631:1646	high performance	1631:1646	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	0	38	theme	Water	0:4	arg1	reclamation					6:16	Water reclamation	0:16	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.	0:121	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.
26043371	7	39	theme	oil/water	1453:1461	arg1	emulsion					1463:1470	a 2000 ppm petroleum oil/water emulsion	1432:1470	a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl	1432:1493	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	2	40	theme	fiber	617:621	arg1	membrane					623:630	The newly developed TFC-FO hollow fiber membrane	583:630	The newly developed TFC-FO hollow fiber membrane	583:630	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	6	41	theme	water	1242:1246	arg1	rinses					1248:1253	water rinses	1242:1253	water rinses on the fiber outer surface	1242:1280	The water flux of the fouled membrane can be effectively restored to 97% of the original value by water rinses on the fiber outer surface without using any chemicals.
26043371	5	42	theme	flux	1049:1052	arg1	%					1072:1072	only 10%	1065:1072	only 10% of the initial value	1065:1093	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	5	42	theme	flux	1049:1052	arg1	value					1089:1093	the initial value	1077:1093	the initial value	1077:1093	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	5	42	theme	flux	1049:1052	arg1	decline					1054:1060	the water flux decline	1039:1060	the water flux decline	1039:1060	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	7	43	theme	2000 ppm	1434:1441	arg1	emulsion					1463:1470	a 2000 ppm petroleum oil/water emulsion	1432:1470	a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl	1432:1493	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	0	44	theme	oily	34:37	arg1	wastewater					39:48	emulsified oily wastewater	23:48	emulsified oily wastewater via effective forward osmosis hollow fiber membranes	23:101	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.
26043371	1	45	theme	polydopamine	276:287	arg1	coating					295:301	polydopamine (PDA) coating	276:301	polydopamine (PDA) coating	276:301	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	6	46	theme	value	1233:1237	arg1	value					1233:1237	the original value	1220:1237	the original value	1220:1237	The water flux of the fouled membrane can be effectively restored to 97% of the original value by water rinses on the fiber outer surface without using any chemicals.
26043371	6	46	theme	value	1233:1237	arg1	%					1215:1215	97%	1213:1215	97% of the original value	1213:1237	The water flux of the fouled membrane can be effectively restored to 97% of the original value by water rinses on the fiber outer surface without using any chemicals.
26043371	1	47	theme	water	458:462	arg1	reclamation					464:474	sustainable water reclamation	446:474	sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO)	446:533	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	48	theme	PDA	290:292	arg1	coating					295:301	polydopamine (PDA) coating	276:301	polydopamine (PDA) coating	276:301	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	49	used	used	437:440	arg2	TFC					403:405	TFC	403:405	TFC	403:405	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	49	used	used	437:440	arg2	membranes					408:416	the thin-film composite (TFC) membranes	378:416	the thin-film composite (TFC) membranes	378:416	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	4	50	theme	Remarkable	960:969	arg1	behaviors					984:992	Remarkable anti-fouling behaviors	960:992	Remarkable anti-fouling behaviors	960:992	Remarkable anti-fouling behaviors have also been observed.
26043371	1	51	theme	oil/water	492:500	arg1	streams					502:508	emulsified oil/water streams	481:508	emulsified oil/water streams via forward osmosis (FO)	481:533	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	5	52	theme	initial	1081:1087	arg1	value					1089:1093	the initial value	1077:1093	the initial value	1077:1093	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	0	53	theme	effective	54:62	arg1	membranes					93:101	effective forward osmosis hollow fiber membranes	54:101	effective forward osmosis hollow fiber membranes	54:101	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.
26043371	8	54	theme	fouling	1656:1662	arg1	behaviors					1664:1672	low fouling behaviors	1652:1672	low fouling behaviors	1652:1672	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	1	55	theme	fiber	225:229	arg1	substrate					231:239	the hollow fiber substrate	214:239	the hollow fiber substrate	214:239	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	3	56	theme	oil/water	894:902	arg1	emulsion					904:911	a 2000 ppm soybean oil/water emulsion	875:911	a 2000 ppm soybean oil/water emulsion	875:911	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	3	56	theme	oil/water	894:902	arg1	NaCl					933:936	the feed and 1 M NaCl	916:936	the feed and 1 M NaCl as the draw solution	916:957	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	6	57	theme	fiber	1262:1266	arg1	surface					1274:1280	the fiber outer surface	1258:1280	the fiber outer surface	1258:1280	The water flux of the fouled membrane can be effectively restored to 97% of the original value by water rinses on the fiber outer surface without using any chemicals.
26043371	3	58	theme	2000 ppm	877:884	arg1	emulsion					904:911	a 2000 ppm soybean oil/water emulsion	875:911	a 2000 ppm soybean oil/water emulsion	875:911	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	3	58	theme	2000 ppm	877:884	arg1	NaCl					933:936	the feed and 1 M NaCl	916:936	the feed and 1 M NaCl as the draw solution	916:957	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	1	59	theme	acetate	162:168	arg1	material					201:208	the membrane material	188:208	the membrane material for the hollow fiber substrate	188:239	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	59	theme	acetate	162:168	arg1	CAB					180:182	CAB	180:182	CAB	180:182	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	59	theme	acetate	162:168	arg1	butyrate					170:177	a novel hydrophilic cellulose acetate butyrate	132:177	a novel hydrophilic cellulose acetate butyrate (CAB)	132:183	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	3	60	theme	feed	920:923	arg1	emulsion					904:911	a 2000 ppm soybean oil/water emulsion	875:911	a 2000 ppm soybean oil/water emulsion	875:911	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	3	60	theme	feed	920:923	arg1	NaCl					933:936	the feed and 1 M NaCl	916:936	the feed and 1 M NaCl as the draw solution	916:957	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	6	61	theme	membrane	1173:1180	arg1	flux					1154:1157	The water flux	1144:1157	The water flux of the fouled membrane	1144:1180	The water flux of the fouled membrane can be effectively restored to 97% of the original value by water rinses on the fiber outer surface without using any chemicals.
26043371	7	62	theme	oil/water	1409:1417	arg1	emulsion					1419:1426	a 2000 ppm soybean oil/water emulsion	1390:1426	a 2000 ppm soybean oil/water emulsion	1390:1426	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	2	63	theme	fouling	718:724	arg1	propensity					726:735	low fouling propensity	714:735	low fouling propensity	714:735	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	8	64	with	fabrication	1584:1594	arg1	behaviors					1664:1672	low fouling behaviors	1652:1672	low fouling behaviors	1652:1672	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	8	64	with	fabrication	1584:1594	arg1	performance					1636:1646	high performance	1631:1646	high performance	1631:1646	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	7	65	theme	2000 ppm	1392:1399	arg1	emulsion					1419:1426	a 2000 ppm soybean oil/water emulsion	1390:1426	a 2000 ppm soybean oil/water emulsion	1390:1426	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	2	66	theme	flux	668:671	arg1	characteristics					638:652	characteristics	638:652	characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity	638:735	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	7	67	contain	containing	1472:1481	arg2	NaCl					1490:1493	0.04 M NaCl	1483:1493	0.04 M NaCl	1483:1493	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	7	67	contain	containing	1472:1481	arg1	emulsion					1463:1470	a 2000 ppm petroleum oil/water emulsion	1432:1470	a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl	1432:1493	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	2	68	theme	high	657:660	arg1	flux					668:671	high water flux	657:671	high water flux	657:671	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	1	69	theme	thin-film	382:390	arg1	membranes					408:416	the thin-film composite (TFC) membranes	378:416	the thin-film composite (TFC) membranes	378:416	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	69	theme	thin-film	382:390	arg1	TFC					403:405	TFC	403:405	TFC	403:405	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	7	70	theme	water	1372:1376	arg1	recovery					1378:1385	the water recovery	1368:1385	the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl	1368:1493	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	4	71	theme	anti-fouling	971:982	arg1	behaviors					984:992	Remarkable anti-fouling behaviors	960:992	Remarkable anti-fouling behaviors	960:992	Remarkable anti-fouling behaviors have also been observed.
26043371	5	72	theme	oil/water	1122:1130	arg1	separation					1132:1141	oil/water separation	1122:1141	oil/water separation	1122:1141	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	0	73	theme	osmosis	72:78	arg1	membranes					93:101	effective forward osmosis hollow fiber membranes	54:101	effective forward osmosis hollow fiber membranes	54:101	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.
26043371	3	74	with	 h	831:832	arg1	rejection					850:858	an oil rejection	843:858	an oil rejection of 99.9%	843:867	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	2	75	theme	outstanding	674:684	arg1	salt					686:689	outstanding salt	674:689	outstanding salt	674:689	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	0	76	theme	fiber	87:91	arg1	membranes					93:101	effective forward osmosis hollow fiber membranes	54:101	effective forward osmosis hollow fiber membranes	54:101	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.
26043371	1	77	theme	hydrophilic	140:150	arg1	material					201:208	the membrane material	188:208	the membrane material for the hollow fiber substrate	188:239	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	77	theme	hydrophilic	140:150	arg1	CAB					180:182	CAB	180:182	CAB	180:182	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	77	theme	hydrophilic	140:150	arg1	butyrate					170:177	a novel hydrophilic cellulose acetate butyrate	132:177	a novel hydrophilic cellulose acetate butyrate (CAB)	132:183	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	3	78	theme	37.1 L m	819:826	arg1	 h					831:832	37.1 L m(-2) h(-1)	819:836	37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution	819:957	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	8	79	theme	PRO	1704:1706	arg1	mode					1708:1711	the PRO mode	1700:1711	the PRO mode	1700:1711	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	2	80	theme	hollow	610:615	arg1	membrane					623:630	The newly developed TFC-FO hollow fiber membrane	583:630	The newly developed TFC-FO hollow fiber membrane	583:630	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	3	81	theme	developed	768:776	arg1	membrane					785:792	the newly developed TFC-FO membrane	758:792	the newly developed TFC-FO membrane	758:792	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	8	82	theme	materials	1774:1782	arg1	design					1760:1765	the design	1756:1765	the design of new materials for water purification purposes	1756:1814	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	3	83	theme	1 M	929:931	arg1	emulsion					904:911	a 2000 ppm soybean oil/water emulsion	875:911	a 2000 ppm soybean oil/water emulsion	875:911	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	3	83	theme	1 M	929:931	arg1	NaCl					933:936	the feed and 1 M NaCl	916:936	the feed and 1 M NaCl as the draw solution	916:957	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	2	84	theme	developed	593:601	arg1	membrane					623:630	The newly developed TFC-FO hollow fiber membrane	583:630	The newly developed TFC-FO hollow fiber membrane	583:630	The newly developed TFC-FO hollow fiber membrane shows characteristics of high water flux, outstanding salt and oil rejection, and low fouling propensity.
26043371	1	85	theme	forward	514:520	arg1	FO					531:532	FO	531:532	FO	531:532	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	85	theme	forward	514:520	arg1	osmosis					522:528	forward osmosis	514:528	forward osmosis (FO)	514:533	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	0	86	theme	PRO	113:115	arg1	mode					117:120	the PRO mode	109:120	the PRO mode	109:120	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.
26043371	1	87	theme	interfacial	324:334	arg1	polymerization					336:349	interfacial polymerization	324:349	interfacial polymerization	324:349	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	8	88	theme	TFC-FO	1609:1614	arg1	membranes					1616:1624	effective TFC-FO membranes	1599:1624	effective TFC-FO membranes	1599:1624	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	3	89	theme	 h	831:832	arg1	flux					811:814	a water flux	803:814	a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution	803:957	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	1	90	from	streams	502:508	arg1	reclamation					464:474	sustainable water reclamation	446:474	sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO)	446:533	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	0	91	from	wastewater	39:48	arg1	reclamation					6:16	Water reclamation	0:16	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.	0:121	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.
26043371	8	92	theme	water	1788:1792	arg1	purposes					1807:1814	water purification purposes	1788:1814	water purification purposes	1788:1814	This study may not only provide insightful guidelines for the fabrication of effective TFC-FO membranes with high performance and low fouling behaviors for oily wastewater under the PRO mode but also add an alternative perspective to the design of new materials for water purification purposes.
26043371	3	93	theme	draw	945:948	arg1	solution					950:957	the draw solution	941:957	the draw solution	941:957	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	7	94	theme	0.04 M	1483:1488	arg1	NaCl					1490:1493	0.04 M NaCl	1483:1493	0.04 M NaCl	1483:1493	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	0	95	theme	emulsified	23:32	arg1	wastewater					39:48	emulsified oily wastewater	23:48	emulsified oily wastewater via effective forward osmosis hollow fiber membranes	23:101	Water reclamation from emulsified oily wastewater via effective forward osmosis hollow fiber membranes under the PRO mode.
26043371	1	96	theme	outer	259:263	arg1	surface					265:271	its outer surface	255:271	its outer surface	255:271	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	7	97	theme	emulsion	1463:1470	arg1	recovery					1378:1385	the water recovery	1368:1385	the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl	1368:1493	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	7	98	theme	petroleum	1443:1451	arg1	emulsion					1463:1470	a 2000 ppm petroleum oil/water emulsion	1432:1470	a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl	1432:1493	Furthermore, the flux declines are only 25% and 52% when the water recovery of a 2000 ppm soybean oil/water emulsion and a 2000 ppm petroleum oil/water emulsion containing 0.04 M NaCl reaches 82%, respectively.
26043371	1	99	theme	sustainable	446:456	arg1	reclamation					464:474	sustainable water reclamation	446:474	sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO)	446:533	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	6	100	theme	original	1224:1231	arg1	value					1233:1237	the original value	1220:1237	the original value	1220:1237	The water flux of the fouled membrane can be effectively restored to 97% of the original value by water rinses on the fiber outer surface without using any chemicals.
26043371	5	101	theme	PRO	1029:1031	arg1	mode					1033:1036	the PRO mode	1025:1036	the PRO mode	1025:1036	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	3	102	theme	oil	846:848	arg1	rejection					850:858	an oil rejection	843:858	an oil rejection of 99.9%	843:867	Under the PRO mode, the newly developed TFC-FO membrane exhibits a water flux of 37.1 L m(-2) h(-1) with an oil rejection of 99.9% using a 2000 ppm soybean oil/water emulsion as the feed and 1 M NaCl as the draw solution.
26043371	1	103	theme	emulsified	481:490	arg1	streams					502:508	emulsified oil/water streams	481:508	emulsified oil/water streams via forward osmosis (FO)	481:533	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	1	104	theme	hollow	218:223	arg1	substrate					231:239	the hollow fiber substrate	214:239	the hollow fiber substrate	214:239	By using a novel hydrophilic cellulose acetate butyrate (CAB) as the membrane material for the hollow fiber substrate and modifying its outer surface by polydopamine (PDA) coating and inner surface by interfacial polymerization, we have demonstrated that the thin-film composite (TFC) membranes can be effectively used for sustainable water reclamation from emulsified oil/water streams via forward osmosis (FO) under the pressure retarded osmosis (PRO) mode.
26043371	5	105	theme	value	1089:1093	arg1	%					1072:1072	only 10%	1065:1072	only 10% of the initial value	1065:1093	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	5	105	theme	value	1089:1093	arg1	decline					1054:1060	the water flux decline	1039:1060	the water flux decline	1039:1060	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
26043371	5	105	theme	value	1089:1093	arg1	value					1089:1093	the initial value	1077:1093	the initial value	1077:1093	Under the PRO mode, the water flux decline is only 10% of the initial value even after a 12 h test for oil/water separation.
24752938	2	0	theme	maximal	535:541	arg1	synergy					550:556	the maximal CBH-EG synergy	531:556	the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw	531:615	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	5	1	theme	bagasse	1161:1167	arg1	hydrolysis					1129:1138	hydrolysis	1129:1138	hydrolysis of alkaline oxidised bagasse	1129:1167	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	4	2	theme	Cel7B	909:913	arg1	proportion					895:904	the high proportion	886:904	the high proportion of Cel7B	886:913	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	4	3	theme	required	939:946	arg1	activity					957:964	the required xylanase activity	935:964	the required xylanase activity	935:964	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	1	4	theme	enzyme	164:169	arg1	components					171:180	five thermostable enzyme components	146:180	five thermostable enzyme components	146:180	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	1	5	theme	oxidised	267:274	arg1	bagasse					287:293	alkaline oxidised sugar cane bagasse	258:293	alkaline oxidised sugar cane bagasse	258:293	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	5	6	theme	optimized	1107:1115	arg1	mixture					1117:1123	the optimized mixture	1103:1123	the optimized mixture	1103:1123	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	1	7	theme	components	171:180	arg1	mixtures					99:106	Optimal enzyme mixtures	84:106	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components	84:180	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	1	8	theme	sugar	276:280	arg1	bagasse					287:293	alkaline oxidised sugar cane bagasse	258:293	alkaline oxidised sugar cane bagasse	258:293	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	5	9	theme	steam-exploded	1174:1187	arg1	bagasse					1189:1195	steam-exploded bagasse	1174:1195	steam-exploded bagasse	1174:1195	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	4	10	theme	enzymes	772:778	arg1	mixture					748:754	The optimal enzyme mixture	729:754	The optimal enzyme mixture of thermostable enzymes	729:778	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	5	11	theme	method	1003:1008	arg1	effect					976:981	The main effect	967:981	The main effect of the pretreatment method	967:1008	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	1	12	theme	cane	282:285	arg1	bagasse					287:293	alkaline oxidised sugar cane bagasse	258:293	alkaline oxidised sugar cane bagasse	258:293	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	4	13	dep	dominated	784:792	arg1	whereas					854:860	whereas	854:860	whereas	854:860	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	2	14	theme	Preliminary	361:371	arg1	studies					373:379	Preliminary studies	361:379	Preliminary studies to narrow down the optimization parameters	361:422	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	2	15	dep	down	391:394	arg1	parameters					413:422	the optimization parameters	396:422	the optimization parameters	396:422	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	1	16	theme	bagasse	287:293	arg1	hydrolysis					205:214	the hydrolysis	201:214	the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments	201:358	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	0	17	from	performance	43:53	arg1	saccharification					66:81	biomass saccharification	58:81	biomass saccharification	58:81	Mixtures of thermostable enzymes show high performance in biomass saccharification.
24752938	5	18	theme	main	971:974	arg1	effect					976:981	The main effect	967:981	The main effect of the pretreatment method	967:1008	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	2	19	theme	cellobiohydrolase/endoglucanase	438:468	arg1	ratio					479:483	a cellobiohydrolase/endoglucanase (CBH/EG) ratio	436:483	a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes	436:524	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	1	20	theme	Optimal	84:90	arg1	mixtures					99:106	Optimal enzyme mixtures	84:106	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components	84:180	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	2	21	theme	higher	495:500	arg1	enzymes					518:524	higher of thermostable enzymes	495:524	higher of thermostable enzymes	495:524	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	5	22	theme	alkaline	1143:1150	arg1	bagasse					1161:1167	alkaline oxidised bagasse	1143:1167	alkaline oxidised bagasse	1143:1167	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	1	23	theme	enzyme	92:97	arg1	mixtures					99:106	Optimal enzyme mixtures	84:106	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components	84:180	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	7	24	theme	commercial	1377:1386	arg1	mixtures					1395:1402	commercial enzyme mixtures	1377:1402	commercial enzyme mixtures	1377:1402	Five-component mixtures of thermostable enzymes showed comparable hydrolysis yields to those of commercial enzyme mixtures.
24752938	7	25	theme	hydrolysis	1347:1356	arg1	yields					1358:1363	comparable hydrolysis yields	1336:1363	comparable hydrolysis yields	1336:1363	Five-component mixtures of thermostable enzymes showed comparable hydrolysis yields to those of commercial enzyme mixtures.
24752938	4	26	theme	high	890:893	arg1	proportion					895:904	the high proportion	886:904	the high proportion of Cel7B	886:913	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	6	27	theme	prolonged	1201:1209	arg1	hydrolyses					1211:1220	prolonged hydrolyses	1201:1220	prolonged hydrolyses	1201:1220	In prolonged hydrolyses, less Cel7A was generally required in the optimal mixture.
24752938	7	28	theme	enzyme	1388:1393	arg1	mixtures					1395:1402	commercial enzyme mixtures	1377:1402	commercial enzyme mixtures	1377:1402	Five-component mixtures of thermostable enzymes showed comparable hydrolysis yields to those of commercial enzyme mixtures.
24752938	0	29	theme	enzymes	25:31	arg1	Mixtures					0:7	Mixtures	0:7	Mixtures of thermostable enzymes	0:31	Mixtures of thermostable enzymes show high performance in biomass saccharification.
24752938	1	30	theme	steam-exploded	299:312	arg1	bagasse					314:320	steam-exploded bagasse	299:320	steam-exploded bagasse	299:320	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	2	31	theme	straw	611:615	arg1	hydrolysis					565:574	the hydrolysis	561:574	the hydrolysis of hydrothermally pretreated wheat straw	561:615	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	4	32	theme	reesei	870:875	arg1	enzymes					877:883	T. reesei enzymes	867:883	T. reesei enzymes	867:883	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	4	33	theme	T.	867:868	arg1	enzymes					877:883	T. reesei enzymes	867:883	T. reesei enzymes	867:883	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	0	34	theme	thermostable	12:23	arg1	enzymes					25:31	thermostable enzymes	12:31	thermostable enzymes	12:31	Mixtures of thermostable enzymes show high performance in biomass saccharification.
24752938	1	35	theme	bagasse	314:320	arg1	hydrolysis					205:214	the hydrolysis	201:214	the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments	201:358	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	2	36	theme	wheat	605:609	arg1	straw					611:615	hydrothermally pretreated wheat straw	579:615	hydrothermally pretreated wheat straw	579:615	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	5	37	theme	pretreatment	990:1001	arg1	method					1003:1008	the pretreatment method	986:1008	the pretreatment method	986:1008	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	0	38	theme	high	38:41	arg1	performance					43:53	high performance	38:53	high performance in biomass saccharification	38:81	Mixtures of thermostable enzymes show high performance in biomass saccharification.
24752938	4	39	theme	thermostable	759:770	arg1	enzymes					772:778	thermostable enzymes	759:778	thermostable enzymes	759:778	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	2	40	theme	pretreated	594:603	arg1	straw					611:615	hydrothermally pretreated wheat straw	579:615	hydrothermally pretreated wheat straw	579:615	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	3	41	theme	enzyme	706:711	arg1	system					713:718	the enzyme system	702:718	the enzyme system studied	702:726	The composition of optimal enzyme mixtures depended clearly on the substrate and on the enzyme system studied.
24752938	3	42	theme	mixtures	652:659	arg1	composition					622:632	The composition	618:632	The composition of optimal enzyme mixtures	618:659	The composition of optimal enzyme mixtures depended clearly on the substrate and on the enzyme system studied.
24752938	2	43	theme	CBH/EG	471:476	arg1	ratio					479:483	a cellobiohydrolase/endoglucanase (CBH/EG) ratio	436:483	a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes	436:524	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	2	44	theme	enzymes	518:524	arg1	ratio					479:483	a cellobiohydrolase/endoglucanase (CBH/EG) ratio	436:483	a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes	436:524	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	2	45	from	synergy	550:556	arg1	hydrolysis					565:574	the hydrolysis	561:574	the hydrolysis of hydrothermally pretreated wheat straw	561:615	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	2	46	theme	4:1	488:490	arg1	ratio					479:483	a cellobiohydrolase/endoglucanase (CBH/EG) ratio	436:483	a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes	436:524	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	1	47	theme	pretreated	234:243	arg1	straw					251:255	hydrothermally pretreated wheat straw	219:255	hydrothermally pretreated wheat straw	219:255	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	2	48	theme	thermostable	505:516	arg1	enzymes					518:524	higher of thermostable enzymes	495:524	higher of thermostable enzymes	495:524	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	1	49	theme	designed	339:346	arg1	experiments					348:358	statistically designed experiments	325:358	statistically designed experiments	325:358	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	2	50	theme	CBH-EG	543:548	arg1	synergy					550:556	the maximal CBH-EG synergy	531:556	the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw	531:615	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	3	51	theme	optimal	637:643	arg1	mixtures					652:659	optimal enzyme mixtures	637:659	optimal enzyme mixtures	637:659	The composition of optimal enzyme mixtures depended clearly on the substrate and on the enzyme system studied.
24752938	2	52	theme	narrow	384:389	arg1	down					391:394	narrow down	384:394	narrow down	384:394	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	2	53	theme	of	502:503	arg1	enzymes					518:524	higher of thermostable enzymes	495:524	higher of thermostable enzymes	495:524	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	3	54	theme	enzyme	645:650	arg1	mixtures					652:659	optimal enzyme mixtures	637:659	optimal enzyme mixtures	637:659	The composition of optimal enzyme mixtures depended clearly on the substrate and on the enzyme system studied.
24752938	7	55	theme	comparable	1336:1345	arg1	yields					1358:1363	comparable hydrolysis yields	1336:1363	comparable hydrolysis yields	1336:1363	Five-component mixtures of thermostable enzymes showed comparable hydrolysis yields to those of commercial enzyme mixtures.
24752938	1	56	theme	reesei	127:132	arg1	enzymes					134:140	six Trichoderma reesei enzymes	111:140	six Trichoderma reesei enzymes	111:140	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	7	57	theme	thermostable	1308:1319	arg1	enzymes					1321:1327	thermostable enzymes	1308:1327	thermostable enzymes	1308:1327	Five-component mixtures of thermostable enzymes showed comparable hydrolysis yields to those of commercial enzyme mixtures.
24752938	4	58	theme	xylanase	948:955	arg1	activity					957:964	the required xylanase activity	935:964	the required xylanase activity	935:964	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	5	59	theme	required	1023:1030	arg1	higher					1059:1064	higher	1059:1064	higher	1059:1064	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	5	59	theme	required	1023:1030	arg1	proportion					1032:1041	the required proportion	1019:1041	the required proportion of xylanase	1019:1053	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	4	60	theme	xylanase	844:851	arg1	xylanase					844:851	xylanase	844:851	xylanase	844:851	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	4	60	theme	xylanase	844:851	arg1	amount					834:839	a relatively high amount	816:839	a relatively high amount of xylanase	816:851	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	4	61	theme	optimal	733:739	arg1	mixture					748:754	The optimal enzyme mixture	729:754	The optimal enzyme mixture of thermostable enzymes	729:778	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	1	62	theme	enzymes	134:140	arg1	mixtures					99:106	Optimal enzyme mixtures	84:106	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components	84:180	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	1	63	theme	wheat	245:249	arg1	straw					251:255	hydrothermally pretreated wheat straw	219:255	hydrothermally pretreated wheat straw	219:255	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	4	64	theme	high	829:832	arg1	xylanase					844:851	xylanase	844:851	xylanase	844:851	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	4	64	theme	high	829:832	arg1	amount					834:839	a relatively high amount	816:839	a relatively high amount of xylanase	816:851	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	5	65	theme	Cel7A	1088:1092	arg1	proportion					1074:1083	the proportion	1070:1083	the proportion of Cel7A	1070:1092	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	2	66	theme	optimization	400:411	arg1	parameters					413:422	the optimization parameters	396:422	the optimization parameters	396:422	Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ratio of 4:1 or higher of thermostable enzymes gave the maximal CBH-EG synergy in the hydrolysis of hydrothermally pretreated wheat straw.
24752938	1	67	theme	straw	251:255	arg1	hydrolysis					205:214	the hydrolysis	201:214	the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments	201:358	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	6	68	theme	optimal	1264:1270	arg1	mixture					1272:1278	the optimal mixture	1260:1278	the optimal mixture	1260:1278	In prolonged hydrolyses, less Cel7A was generally required in the optimal mixture.
24752938	4	69	theme	enzyme	741:746	arg1	mixture					748:754	The optimal enzyme mixture	729:754	The optimal enzyme mixture of thermostable enzymes	729:778	The optimal enzyme mixture of thermostable enzymes was dominated by Cel7A and required a relatively high amount of xylanase, whereas with T. reesei enzymes, the high proportion of Cel7B appeared to provide the required xylanase activity.
24752938	0	70	theme	biomass	58:64	arg1	saccharification					66:81	biomass saccharification	58:81	biomass saccharification	58:81	Mixtures of thermostable enzymes show high performance in biomass saccharification.
24752938	5	71	theme	oxidised	1152:1159	arg1	bagasse					1161:1167	alkaline oxidised bagasse	1143:1167	alkaline oxidised bagasse	1143:1167	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	5	72	theme	xylanase	1046:1053	arg1	higher					1059:1064	higher	1059:1064	higher	1059:1064	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	5	72	theme	xylanase	1046:1053	arg1	proportion					1032:1041	the required proportion	1019:1041	the required proportion of xylanase	1019:1053	The main effect of the pretreatment method was that the required proportion of xylanase was higher and the proportion of Cel7A lower in the optimized mixture for hydrolysis of alkaline oxidised bagasse than steam-exploded bagasse.
24752938	7	73	theme	enzymes	1321:1327	arg1	mixtures					1296:1303	Five-component mixtures	1281:1303	Five-component mixtures of thermostable enzymes	1281:1327	Five-component mixtures of thermostable enzymes showed comparable hydrolysis yields to those of commercial enzyme mixtures.
24752938	1	74	theme	thermostable	151:162	arg1	components					171:180	five thermostable enzyme components	146:180	five thermostable enzyme components	146:180	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
24752938	7	75	theme	Five-component	1281:1294	arg1	mixtures					1296:1303	Five-component mixtures	1281:1303	Five-component mixtures of thermostable enzymes	1281:1327	Five-component mixtures of thermostable enzymes showed comparable hydrolysis yields to those of commercial enzyme mixtures.
24752938	1	76	theme	alkaline	258:265	arg1	bagasse					287:293	alkaline oxidised sugar cane bagasse	258:293	alkaline oxidised sugar cane bagasse	258:293	Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments.
25723913	5	0	theme	MTM	797:799	arg1	materials					802:810	CNF/montmorrilonite (MTM) materials	776:810	CNF/montmorrilonite (MTM) materials	776:810	These two tests provide a wide spectrum characterization of fire protection properties in CNF/montmorrilonite (MTM) materials.
25723913	8	1	theme	barrier	1188:1194	arg1	nanostructure					1131:1143	unique nanostructure	1124:1143	unique nanostructure	1124:1143	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	8	1	theme	barrier	1188:1194	arg1	properties					1196:1205	high gas barrier properties	1179:1205	high gas barrier properties	1179:1205	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	8	2	theme	chemical	1070:1077	arg1	mechanisms					1079:1088	Physical and chemical mechanisms	1057:1088	Physical and chemical mechanisms	1057:1088	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	7	3	theme	oxidative	1028:1036	arg1	atmospheres					1044:1054	inert (nitrogen) and oxidative (air) atmospheres	1007:1054	inert (nitrogen) and oxidative (air) atmospheres	1007:1054	In addition, thermal and thermo-oxidative stability are evaluated by thermogravimetric analyses performed in inert (nitrogen) and oxidative (air) atmospheres.
25723913	7	3	theme	oxidative	1028:1036	arg1	air					1039:1041	air	1039:1041	air	1039:1041	In addition, thermal and thermo-oxidative stability are evaluated by thermogravimetric analyses performed in inert (nitrogen) and oxidative (air) atmospheres.
25723913	7	4	theme	thermo-oxidative	923:938	arg1	stability					940:948	thermo-oxidative stability	923:948	thermo-oxidative stability	923:948	In addition, thermal and thermo-oxidative stability are evaluated by thermogravimetric analyses performed in inert (nitrogen) and oxidative (air) atmospheres.
25723913	5	5	from	characterization	726:741	arg1	materials					802:810	CNF/montmorrilonite (MTM) materials	776:810	CNF/montmorrilonite (MTM) materials	776:810	These two tests provide a wide spectrum characterization of fire protection properties in CNF/montmorrilonite (MTM) materials.
25723913	8	6	theme	gas	1184:1186	arg1	nanostructure					1131:1143	unique nanostructure	1124:1143	unique nanostructure	1124:1143	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	8	6	theme	gas	1184:1186	arg1	properties					1196:1205	high gas barrier properties	1179:1205	high gas barrier properties	1179:1205	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	4	7	theme	corresponding	554:566	arg1	mechanisms					568:577	The corresponding mechanisms	550:577	The corresponding mechanisms	550:577	The corresponding mechanisms are evaluated in terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux).
25723913	6	8	theme	flammability	860:871	arg1	testing					873:879	flammability testing	860:879	flammability testing	860:879	The morphology of the collected residues after flammability testing is investigated.
25723913	6	9	theme	residues	845:852	arg1	morphology					817:826	The morphology	813:826	The morphology of the collected residues after flammability testing	813:879	The morphology of the collected residues after flammability testing is investigated.
25723913	1	10	theme	major	167:171	arg1	toxicity					106:113	The toxicity	102:113	The toxicity of the most efficient fire retardant additives	102:160	The toxicity of the most efficient fire retardant additives is a major problem for polymeric materials.
25723913	1	10	theme	major	167:171	arg1	problem					173:179	a major problem	165:179	a major problem for polymeric materials	165:203	The toxicity of the most efficient fire retardant additives is a major problem for polymeric materials.
25723913	4	11	theme	reaction	619:626	arg1	flame					633:637	reaction to a flame	619:637	reaction to a flame	619:637	The corresponding mechanisms are evaluated in terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux).
25723913	4	11	theme	reaction	619:626	arg1	flammability					605:616	flammability	605:616	flammability (reaction to a flame)	605:638	The corresponding mechanisms are evaluated in terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux).
25723913	5	12	theme	fire	746:749	arg1	properties					762:771	fire protection properties	746:771	fire protection properties	746:771	These two tests provide a wide spectrum characterization of fire protection properties in CNF/montmorrilonite (MTM) materials.
25723913	6	13	theme	collected	835:843	arg1	residues					845:852	the collected residues	831:852	the collected residues	831:852	The morphology of the collected residues after flammability testing is investigated.
25723913	5	14	theme	protection	751:760	arg1	properties					762:771	fire protection properties	746:771	fire protection properties	746:771	These two tests provide a wide spectrum characterization of fire protection properties in CNF/montmorrilonite (MTM) materials.
25723913	7	15	theme	inert	1007:1011	arg1	atmospheres					1044:1054	inert (nitrogen) and oxidative (air) atmospheres	1007:1054	inert (nitrogen) and oxidative (air) atmospheres	1007:1054	In addition, thermal and thermo-oxidative stability are evaluated by thermogravimetric analyses performed in inert (nitrogen) and oxidative (air) atmospheres.
25723913	7	15	theme	inert	1007:1011	arg1	air					1039:1041	air	1039:1041	air	1039:1041	In addition, thermal and thermo-oxidative stability are evaluated by thermogravimetric analyses performed in inert (nitrogen) and oxidative (air) atmospheres.
25723913	4	16	theme	a	631:631	arg1	flame					633:637	reaction to a flame	619:637	reaction to a flame	619:637	The corresponding mechanisms are evaluated in terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux).
25723913	4	16	theme	a	631:631	arg1	flammability					605:616	flammability	605:616	flammability (reaction to a flame)	605:638	The corresponding mechanisms are evaluated in terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux).
25723913	5	17	theme	properties	762:771	arg1	characterization					726:741	a wide spectrum characterization	710:741	a wide spectrum characterization of fire protection properties in CNF/montmorrilonite (MTM) materials	710:810	These two tests provide a wide spectrum characterization of fire protection properties in CNF/montmorrilonite (MTM) materials.
25723913	2	18	theme	electron	394:401	arg1	microscopy					403:412	scanning electron microscopy	385:412	scanning electron microscopy	385:412	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	3	19	theme	superior	463:470	arg1	properties					488:497	superior fire protection properties	463:497	superior fire protection properties	463:497	These nanocomposites have superior fire protection properties to other clay nanocomposites and fiber composites.
25723913	8	20	theme	CNF/MTM	1211:1217	arg1	nanostructure					1131:1143	unique nanostructure	1124:1143	unique nanostructure	1124:1143	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	8	20	theme	CNF/MTM	1211:1217	arg1	interactions					1219:1230	CNF/MTM interactions	1211:1230	CNF/MTM interactions for char formation	1211:1249	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	3	21	theme	fire	472:475	arg1	properties					488:497	superior fire protection properties	463:497	superior fire protection properties	463:497	These nanocomposites have superior fire protection properties to other clay nanocomposites and fiber composites.
25723913	8	22	theme	char	1236:1239	arg1	formation					1241:1249	char formation	1236:1249	char formation	1236:1249	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	4	23	theme	flammability	605:616	arg1	terms					596:600	terms	596:600	terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux)	596:683	The corresponding mechanisms are evaluated in terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux).
25723913	5	24	theme	CNF/montmorrilonite	776:794	arg1	materials					802:810	CNF/montmorrilonite (MTM) materials	776:810	CNF/montmorrilonite (MTM) materials	776:810	These two tests provide a wide spectrum characterization of fire protection properties in CNF/montmorrilonite (MTM) materials.
25723913	1	25	theme	polymeric	185:193	arg1	materials					195:203	polymeric materials	185:203	polymeric materials	185:203	The toxicity of the most efficient fire retardant additives is a major problem for polymeric materials.
25723913	0	26	theme	Oriented	0:7	arg1	clay					9:12	Oriented clay	0:12	Oriented clay	0:12	Oriented clay nanopaper from biobased components--mechanisms for superior fire protection properties.
25723913	7	27	theme	thermogravimetric	967:983	arg1	analyses					985:992	thermogravimetric analyses	967:992	thermogravimetric analyses performed in inert (nitrogen) and oxidative (air) atmospheres	967:1054	In addition, thermal and thermo-oxidative stability are evaluated by thermogravimetric analyses performed in inert (nitrogen) and oxidative (air) atmospheres.
25723913	4	28	dep	calorimetry	649:659	arg1	exposure					662:669	exposure	662:669	exposure to heat flux	662:682	The corresponding mechanisms are evaluated in terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux).
25723913	2	29	theme	scanning	385:392	arg1	microscopy					403:412	scanning electron microscopy	385:412	scanning electron microscopy	385:412	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	8	30	theme	Physical	1057:1064	arg1	mechanisms					1079:1088	Physical and chemical mechanisms	1057:1088	Physical and chemical mechanisms	1057:1088	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	5	31	theme	wide	712:715	arg1	spectrum					717:724	a wide spectrum	710:724	a wide spectrum characterization of fire protection properties in CNF/montmorrilonite (MTM) materials	710:810	These two tests provide a wide spectrum characterization of fire protection properties in CNF/montmorrilonite (MTM) materials.
25723913	8	32	theme	high	1179:1182	arg1	nanostructure					1131:1143	unique nanostructure	1124:1143	unique nanostructure	1124:1143	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	8	32	theme	high	1179:1182	arg1	properties					1196:1205	high gas barrier properties	1179:1205	high gas barrier properties	1179:1205	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	2	33	with	nanocomposites	237:250	arg1	prepared					296:303	prepared	296:303	prepared by simple filtration	296:324	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	2	33	with	nanocomposites	237:250	arg1	structure					282:290	unique brick-and-mortar structure	258:290	unique brick-and-mortar structure	258:290	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	5	34	theme	spectrum	717:724	arg1	characterization					726:741	a wide spectrum characterization	710:741	a wide spectrum characterization of fire protection properties in CNF/montmorrilonite (MTM) materials	710:810	These two tests provide a wide spectrum characterization of fire protection properties in CNF/montmorrilonite (MTM) materials.
25723913	4	35	dep	flame	633:637	arg1	to					628:629	to	628:629	to	628:629	The corresponding mechanisms are evaluated in terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux).
25723913	2	36	theme	simple	308:313	arg1	filtration					315:324	simple filtration	308:324	simple filtration	308:324	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	3	37	theme	other	502:506	arg1	nanocomposites					513:526	other clay nanocomposites	502:526	other clay nanocomposites	502:526	These nanocomposites have superior fire protection properties to other clay nanocomposites and fiber composites.
25723913	3	38	theme	fiber	532:536	arg1	composites					538:547	fiber composites	532:547	fiber composites	532:547	These nanocomposites have superior fire protection properties to other clay nanocomposites and fiber composites.
25723913	4	39	theme	heat	674:677	arg1	flux					679:682	heat flux	674:682	heat flux	674:682	The corresponding mechanisms are evaluated in terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux).
25723913	3	40	theme	protection	477:486	arg1	properties					488:497	superior fire protection properties	463:497	superior fire protection properties	463:497	These nanocomposites have superior fire protection properties to other clay nanocomposites and fiber composites.
25723913	0	41	theme	biobased	29:36	arg1	components--mechanisms					38:59	biobased components--mechanisms	29:59	biobased components--mechanisms for superior fire protection properties	29:99	Oriented clay nanopaper from biobased components--mechanisms for superior fire protection properties.
25723913	2	42	theme	/clay	231:235	arg1	nanocomposites					237:250	Cellulose nanofiber (CNF)/clay nanocomposites	206:250	Cellulose nanofiber (CNF)/clay nanocomposites	206:250	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	2	43	theme	X-ray	418:422	arg1	diffraction					424:434	X-ray diffraction	418:434	X-ray diffraction	418:434	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	0	44	theme	superior	65:72	arg1	properties					90:99	superior fire protection properties	65:99	superior fire protection properties	65:99	Oriented clay nanopaper from biobased components--mechanisms for superior fire protection properties.
25723913	8	45	theme	low	1153:1155	arg1	conductivity					1165:1176	its low thermal conductivity	1149:1176	its low thermal conductivity	1149:1176	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	2	46	theme	morphological	354:366	arg1	point					368:372	the morphological point	350:372	the morphological point of view	350:380	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	8	47	theme	unique	1124:1129	arg1	nanostructure					1131:1143	unique nanostructure	1124:1143	unique nanostructure	1124:1143	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	8	47	theme	unique	1124:1129	arg1	properties					1196:1205	high gas barrier properties	1179:1205	high gas barrier properties	1179:1205	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	8	47	theme	unique	1124:1129	arg1	interactions					1219:1230	CNF/MTM interactions	1211:1230	CNF/MTM interactions for char formation	1211:1249	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	3	48	contain	have	458:461	arg1	nanocomposites					443:456	These nanocomposites	437:456	These nanocomposites	437:456	These nanocomposites have superior fire protection properties to other clay nanocomposites and fiber composites.
25723913	3	48	contain	have	458:461	arg2	properties					488:497	superior fire protection properties	463:497	superior fire protection properties	463:497	These nanocomposites have superior fire protection properties to other clay nanocomposites and fiber composites.
25723913	1	49	theme	efficient	127:135	arg1	additives					152:160	the most efficient fire retardant additives	118:160	the most efficient fire retardant additives	118:160	The toxicity of the most efficient fire retardant additives is a major problem for polymeric materials.
25723913	7	50	dep	inert	1007:1011	arg1	nitrogen					1014:1021	nitrogen	1014:1021	nitrogen	1014:1021	In addition, thermal and thermo-oxidative stability are evaluated by thermogravimetric analyses performed in inert (nitrogen) and oxidative (air) atmospheres.
25723913	2	51	theme	nanofiber	216:224	arg1	nanocomposites					237:250	Cellulose nanofiber (CNF)/clay nanocomposites	206:250	Cellulose nanofiber (CNF)/clay nanocomposites	206:250	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	2	52	theme	brick-and-mortar	265:280	arg1	structure					282:290	unique brick-and-mortar structure	258:290	unique brick-and-mortar structure	258:290	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	1	53	theme	fire	137:140	arg1	additives					152:160	the most efficient fire retardant additives	118:160	the most efficient fire retardant additives	118:160	The toxicity of the most efficient fire retardant additives is a major problem for polymeric materials.
25723913	8	54	theme	thermal	1157:1163	arg1	conductivity					1165:1176	its low thermal conductivity	1149:1176	its low thermal conductivity	1149:1176	Physical and chemical mechanisms are identified and related to the unique nanostructure and its low thermal conductivity, high gas barrier properties and CNF/MTM interactions for char formation.
25723913	2	55	theme	Cellulose	206:214	arg1	CNF					227:229	CNF	227:229	CNF	227:229	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	2	55	theme	Cellulose	206:214	arg1	nanofiber					216:224	Cellulose nanofiber	206:224	Cellulose nanofiber (CNF)/clay nanocomposites	206:250	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	2	56	theme	unique	258:263	arg1	structure					282:290	unique brick-and-mortar structure	258:290	unique brick-and-mortar structure	258:290	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	1	57	theme	retardant	142:150	arg1	additives					152:160	the most efficient fire retardant additives	118:160	the most efficient fire retardant additives	118:160	The toxicity of the most efficient fire retardant additives is a major problem for polymeric materials.
25723913	3	58	theme	clay	508:511	arg1	nanocomposites					513:526	other clay nanocomposites	502:526	other clay nanocomposites	502:526	These nanocomposites have superior fire protection properties to other clay nanocomposites and fiber composites.
25723913	0	59	theme	protection	79:88	arg1	properties					90:99	superior fire protection properties	65:99	superior fire protection properties	65:99	Oriented clay nanopaper from biobased components--mechanisms for superior fire protection properties.
25723913	1	60	theme	additives	152:160	arg1	toxicity					106:113	The toxicity	102:113	The toxicity of the most efficient fire retardant additives	102:160	The toxicity of the most efficient fire retardant additives is a major problem for polymeric materials.
25723913	1	60	theme	additives	152:160	arg1	problem					173:179	a major problem	165:179	a major problem for polymeric materials	165:203	The toxicity of the most efficient fire retardant additives is a major problem for polymeric materials.
25723913	4	61	theme	calorimetry	649:659	arg1	terms					596:600	terms	596:600	terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux)	596:683	The corresponding mechanisms are evaluated in terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux).
25723913	0	62	theme	fire	74:77	arg1	properties					90:99	superior fire protection properties	65:99	superior fire protection properties	65:99	Oriented clay nanopaper from biobased components--mechanisms for superior fire protection properties.
25723913	2	63	theme	view	377:380	arg1	point					368:372	the morphological point	350:372	the morphological point of view	350:380	Cellulose nanofiber (CNF)/clay nanocomposites, with unique brick-and-mortar structure and prepared by simple filtration, are characterized from the morphological point of view by scanning electron microscopy and X-ray diffraction.
25723913	4	64	theme	cone	644:647	arg1	calorimetry					649:659	cone calorimetry	644:659	cone calorimetry (exposure to heat flux)	644:683	The corresponding mechanisms are evaluated in terms of flammability (reaction to a flame) and cone calorimetry (exposure to heat flux).
27474660	3	0	theme	@	519:519	arg1	biocomposites					503:515	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	0	theme	@	519:519	arg1	AlgBent					520:526	Ca@AlgBent	517:526	Ca@AlgBent	517:526	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	1	1	from	clay	158:161	arg1	biopolymer					168:177	a biopolymer	166:177	a biopolymer	166:177	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	0	2	theme	Cr	79:80	arg1	sorption					86:93	Cr(VI) sorption	79:93	Cr(VI) sorption	79:93	Synthesis of assorted metal ions anchored alginate bentonite biocomposites for Cr(VI) sorption.
27474660	4	3	dep	concentration	746:758	arg1	VI					742:743	VI	742:743	VI	742:743	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	1	4	theme	Ca	291:292	arg1	AlgBent					294:300	Ca@AlgBent	291:300	Ca@AlgBent	291:300	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	1	5	theme	Bent	152:155	arg1	clay					158:161	bentonite (Bent) clay	141:161	bentonite (Bent) clay in a biopolymer namely alginate (Alg)	141:199	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	8	6	from	conditions	1231:1240	arg1	application					1189:1199	The application	1185:1199	The application of the biocomposites at field conditions	1185:1240	The application of the biocomposites at field conditions was also examined by testing it with industrial water.
27474660	2	7	theme	synthesized	358:368	arg1	biocomposites					370:382	The synthesized biocomposites	354:382	The synthesized biocomposites	354:382	The synthesized biocomposites were characterized by various instrumental techniques like FTIR, SEM and EDAX.
27474660	3	8	theme	biocomposites	503:515	arg1	Cr					463:464	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	463:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	463:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	4	9	theme	sorbent	660:666	arg1	concentration					746:758	initial Cr(VI) concentration	731:758	initial Cr(VI) concentration	731:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	9	theme	sorbent	660:666	arg1	temperature					687:697	temperature	687:697	temperature	687:697	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	9	theme	sorbent	660:666	arg1	time					640:643	contact time	632:643	contact time	632:643	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	9	theme	sorbent	660:666	arg1	pH					669:670	pH	669:670	pH of the medium	669:684	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	9	theme	sorbent	660:666	arg1	presence					700:707	presence	700:707	presence of common co-ions	700:725	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	9	theme	sorbent	660:666	arg1	factors					618:624	Various adsorption influencing factors	587:624	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration	587:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	9	theme	sorbent	660:666	arg1	dosage					646:651	dosage	646:651	dosage of the sorbent	646:666	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	9	theme	sorbent	660:666	arg1	viz.					626:629	viz.	626:629	viz.	626:629	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	1	10	theme	@	293:293	arg1	AlgBent					294:300	Ca@AlgBent	291:300	Ca@AlgBent	291:300	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	8	11	theme	biocomposites	1208:1220	arg1	application					1189:1199	The application	1185:1199	The application of the biocomposites at field conditions	1185:1240	The application of the biocomposites at field conditions was also examined by testing it with industrial water.
27474660	9	12	theme	regeneration	1301:1312	arg1	studies					1314:1320	The regeneration studies	1297:1320	The regeneration studies	1297:1320	The regeneration studies were carried to know about the reusability of the biocomposites.
27474660	3	13	theme	Ce	529:530	arg1	AlgBent					532:538	Ce@AlgBent	529:538	Ce@AlgBent	529:538	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	13	theme	Ce	529:530	arg1	biocomposites					503:515	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	0	14	dep	sorption	86:93	arg1	VI					82:83	VI	82:83	VI	82:83	Synthesis of assorted metal ions anchored alginate bentonite biocomposites for Cr(VI) sorption.
27474660	4	15	theme	co-ions	719:725	arg1	concentration					746:758	initial Cr(VI) concentration	731:758	initial Cr(VI) concentration	731:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	15	theme	co-ions	719:725	arg1	temperature					687:697	temperature	687:697	temperature	687:697	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	15	theme	co-ions	719:725	arg1	time					640:643	contact time	632:643	contact time	632:643	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	15	theme	co-ions	719:725	arg1	pH					669:670	pH	669:670	pH of the medium	669:684	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	15	theme	co-ions	719:725	arg1	presence					700:707	presence	700:707	presence of common co-ions	700:725	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	15	theme	co-ions	719:725	arg1	factors					618:624	Various adsorption influencing factors	587:624	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration	587:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	15	theme	co-ions	719:725	arg1	dosage					646:651	dosage	646:651	dosage of the sorbent	646:666	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	15	theme	co-ions	719:725	arg1	viz.					626:629	viz.	626:629	viz.	626:629	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	3	16	theme	@	531:531	arg1	AlgBent					532:538	Ce@AlgBent	529:538	Ce@AlgBent	529:538	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	16	theme	@	531:531	arg1	biocomposites					503:515	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	4	17	theme	contact	632:638	arg1	time					640:643	contact time	632:643	contact time	632:643	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	17	theme	contact	632:638	arg1	factors					618:624	Various adsorption influencing factors	587:624	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration	587:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	18	theme	common	712:717	arg1	co-ions					719:725	common co-ions	712:725	common co-ions	712:725	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	1	19	theme	Ce	303:304	arg1	AlgBent					306:312	Ce@AlgBent	303:312	Ce@AlgBent	303:312	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	6	20	theme	process	919:925	arg1	Kinetics					894:901	Kinetics	894:901	Kinetics of the sorption process	894:925	Kinetics of the sorption process was carried out by pseudo-first-order and pseudo-second-order models.
27474660	8	21	theme	industrial	1279:1288	arg1	water					1290:1294	industrial water	1279:1294	industrial water	1279:1294	The application of the biocomposites at field conditions was also examined by testing it with industrial water.
27474660	4	22	theme	Cr	739:740	arg1	concentration					746:758	initial Cr(VI) concentration	731:758	initial Cr(VI) concentration	731:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	22	theme	Cr	739:740	arg1	factors					618:624	Various adsorption influencing factors	587:624	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration	587:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	1	23	theme	@	305:305	arg1	AlgBent					306:312	Ce@AlgBent	303:312	Ce@AlgBent	303:312	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	6	24	theme	sorption	910:917	arg1	process					919:925	the sorption process	906:925	the sorption process	906:925	Kinetics of the sorption process was carried out by pseudo-first-order and pseudo-second-order models.
27474660	6	25	theme	pseudo-first-order	946:963	arg1	models					989:994	pseudo-first-order and pseudo-second-order models	946:994	pseudo-first-order and pseudo-second-order models	946:994	Kinetics of the sorption process was carried out by pseudo-first-order and pseudo-second-order models.
27474660	3	26	theme	Zr	544:545	arg1	biocomposites					503:515	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	26	theme	Zr	544:545	arg1	AlgBent					547:553	Zr@AlgBent	544:553	Zr@AlgBent	544:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	8	27	theme	field	1225:1229	arg1	conditions					1231:1240	field conditions	1225:1240	field conditions	1225:1240	The application of the biocomposites at field conditions was also examined by testing it with industrial water.
27474660	5	28	theme	Langmuir	786:793	arg1	models					835:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models	774:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models	774:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models were adopted to examine the adsorption equilibrium.
27474660	7	29	dep	Cr	1144:1145	arg1	VI					1147:1148	VI	1147:1148	VI	1147:1148	The nature of the sorption process was explained using thermodynamic parameters like ΔS°, ΔG° and ΔH° and a possible mechanism for the sorption of Cr(VI) onto the biocomposites was given.
27474660	0	30	theme	metal	22:26	arg1	ions					28:31	assorted metal ions	13:31	assorted metal ions	13:31	Synthesis of assorted metal ions anchored alginate bentonite biocomposites for Cr(VI) sorption.
27474660	3	31	theme	@	546:546	arg1	biocomposites					503:515	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	31	theme	@	546:546	arg1	AlgBent					547:553	Zr@AlgBent	544:553	Zr@AlgBent	544:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	0	32	theme	assorted	13:20	arg1	ions					28:31	assorted metal ions	13:31	assorted metal ions	13:31	Synthesis of assorted metal ions anchored alginate bentonite biocomposites for Cr(VI) sorption.
27474660	5	33	theme	adsorption	870:879	arg1	equilibrium					881:891	the adsorption equilibrium	866:891	the adsorption equilibrium	866:891	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models were adopted to examine the adsorption equilibrium.
27474660	4	34	theme	medium	679:684	arg1	concentration					746:758	initial Cr(VI) concentration	731:758	initial Cr(VI) concentration	731:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	34	theme	medium	679:684	arg1	temperature					687:697	temperature	687:697	temperature	687:697	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	34	theme	medium	679:684	arg1	time					640:643	contact time	632:643	contact time	632:643	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	34	theme	medium	679:684	arg1	pH					669:670	pH	669:670	pH of the medium	669:684	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	34	theme	medium	679:684	arg1	presence					700:707	presence	700:707	presence of common co-ions	700:725	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	34	theme	medium	679:684	arg1	factors					618:624	Various adsorption influencing factors	587:624	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration	587:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	34	theme	medium	679:684	arg1	dosage					646:651	dosage	646:651	dosage of the sorbent	646:666	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	34	theme	medium	679:684	arg1	viz.					626:629	viz.	626:629	viz.	626:629	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	3	35	theme	sorption	470:477	arg1	SCs					491:493	SCs	491:493	SCs	491:493	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	35	theme	sorption	470:477	arg1	capacities					479:488	sorption capacities	470:488	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	463:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	4	36	dep	factors	618:624	arg1	concentration					746:758	initial Cr(VI) concentration	731:758	initial Cr(VI) concentration	731:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	36	dep	factors	618:624	arg1	temperature					687:697	temperature	687:697	temperature	687:697	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	36	dep	factors	618:624	arg1	time					640:643	contact time	632:643	contact time	632:643	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	36	dep	factors	618:624	arg1	pH					669:670	pH	669:670	pH of the medium	669:684	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	36	dep	factors	618:624	arg1	presence					700:707	presence	700:707	presence of common co-ions	700:725	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	36	dep	factors	618:624	arg1	factors					618:624	Various adsorption influencing factors	587:624	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration	587:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	36	dep	factors	618:624	arg1	dosage					646:651	dosage	646:651	dosage of the sorbent	646:666	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	36	dep	factors	618:624	arg1	viz.					626:629	viz.	626:629	viz.	626:629	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	37	theme	influencing	606:616	arg1	concentration					746:758	initial Cr(VI) concentration	731:758	initial Cr(VI) concentration	731:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	37	theme	influencing	606:616	arg1	temperature					687:697	temperature	687:697	temperature	687:697	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	37	theme	influencing	606:616	arg1	time					640:643	contact time	632:643	contact time	632:643	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	37	theme	influencing	606:616	arg1	pH					669:670	pH	669:670	pH of the medium	669:684	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	37	theme	influencing	606:616	arg1	presence					700:707	presence	700:707	presence of common co-ions	700:725	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	37	theme	influencing	606:616	arg1	factors					618:624	Various adsorption influencing factors	587:624	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration	587:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	37	theme	influencing	606:616	arg1	dosage					646:651	dosage	646:651	dosage of the sorbent	646:666	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	37	theme	influencing	606:616	arg1	viz.					626:629	viz.	626:629	viz.	626:629	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	3	38	dep	Cr	463:464	arg1	SCs					491:493	SCs	491:493	SCs	491:493	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	38	dep	Cr	463:464	arg1	VI					466:467	VI	466:467	VI	466:467	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	38	dep	Cr	463:464	arg1	capacities					479:488	sorption capacities	470:488	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	463:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	0	39	theme	ions	28:31	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of assorted metal ions	0:31	Synthesis of assorted metal ions anchored alginate bentonite biocomposites for Cr(VI) sorption.
27474660	5	40	theme	isotherm	826:833	arg1	models					835:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models	774:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models	774:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models were adopted to examine the adsorption equilibrium.
27474660	4	41	theme	adsorption	595:604	arg1	concentration					746:758	initial Cr(VI) concentration	731:758	initial Cr(VI) concentration	731:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	41	theme	adsorption	595:604	arg1	temperature					687:697	temperature	687:697	temperature	687:697	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	41	theme	adsorption	595:604	arg1	time					640:643	contact time	632:643	contact time	632:643	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	41	theme	adsorption	595:604	arg1	pH					669:670	pH	669:670	pH of the medium	669:684	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	41	theme	adsorption	595:604	arg1	presence					700:707	presence	700:707	presence of common co-ions	700:725	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	41	theme	adsorption	595:604	arg1	factors					618:624	Various adsorption influencing factors	587:624	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration	587:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	41	theme	adsorption	595:604	arg1	dosage					646:651	dosage	646:651	dosage of the sorbent	646:666	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	41	theme	adsorption	595:604	arg1	viz.					626:629	viz.	626:629	viz.	626:629	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	1	42	theme	Zr	318:319	arg1	composites					329:338	Zr@AlgBent composites	318:338	Zr@AlgBent composites	318:338	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	0	43	theme	bentonite	51:59	arg1	biocomposites					61:73	alginate bentonite biocomposites	42:73	alginate bentonite biocomposites for Cr(VI) sorption	42:93	Synthesis of assorted metal ions anchored alginate bentonite biocomposites for Cr(VI) sorption.
27474660	3	44	dep	biocomposites	503:515	arg1	AlgBent					532:538	Ce@AlgBent	529:538	Ce@AlgBent	529:538	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	44	dep	biocomposites	503:515	arg1	AlgBent					520:526	Ca@AlgBent	517:526	Ca@AlgBent	517:526	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	44	dep	biocomposites	503:515	arg1	AlgBent					547:553	Zr@AlgBent	544:553	Zr@AlgBent	544:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	44	dep	biocomposites	503:515	arg1	biocomposites					503:515	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	5	45	theme	Freundlich	774:783	arg1	models					835:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models	774:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models	774:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models were adopted to examine the adsorption equilibrium.
27474660	1	46	theme	@	320:320	arg1	composites					329:338	Zr@AlgBent composites	318:338	Zr@AlgBent composites	318:338	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	0	47	theme	alginate	42:49	arg1	biocomposites					61:73	alginate bentonite biocomposites	42:73	alginate bentonite biocomposites for Cr(VI) sorption	42:93	Synthesis of assorted metal ions anchored alginate bentonite biocomposites for Cr(VI) sorption.
27474660	1	48	theme	metal	274:278	arg1	ions					280:283	metal ions	274:283	metal ions	274:283	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	1	49	dep	clay	158:161	arg1	Alg					196:198	Alg	196:198	Alg	196:198	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	1	49	dep	clay	158:161	arg1	alginate					186:193	alginate	186:193	bentonite (Bent) clay in a biopolymer namely alginate (Alg)	141:199	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	1	50	theme	AlgBent	321:327	arg1	composites					329:338	Zr@AlgBent composites	318:338	Zr@AlgBent composites	318:338	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	7	51	theme	thermodynamic	1052:1064	arg1	parameters					1066:1075	thermodynamic parameters	1052:1075	thermodynamic parameters like ΔS°, ΔG° and ΔH°	1052:1097	The nature of the sorption process was explained using thermodynamic parameters like ΔS°, ΔG° and ΔH° and a possible mechanism for the sorption of Cr(VI) onto the biocomposites was given.
27474660	1	52	dep	tetravalent	253:263	arg1	4+					269:270	4+	269:270	4+	269:270	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	1	52	dep	tetravalent	253:263	arg1	Zr					266:267	Zr	266:267	Zr(4+)	266:271	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	2	53	theme	instrumental	414:425	arg1	techniques					427:436	various instrumental techniques	406:436	various instrumental techniques like FTIR, SEM and EDAX	406:460	The synthesized biocomposites were characterized by various instrumental techniques like FTIR, SEM and EDAX.
27474660	6	54	theme	pseudo-second-order	969:987	arg1	models					989:994	pseudo-first-order and pseudo-second-order models	946:994	pseudo-first-order and pseudo-second-order models	946:994	Kinetics of the sorption process was carried out by pseudo-first-order and pseudo-second-order models.
27474660	7	55	theme	Cr	1144:1145	arg1	sorption					1132:1139	the sorption	1128:1139	the sorption of Cr(VI) onto the biocomposites	1128:1172	The nature of the sorption process was explained using thermodynamic parameters like ΔS°, ΔG° and ΔH° and a possible mechanism for the sorption of Cr(VI) onto the biocomposites was given.
27474660	2	56	theme	various	406:412	arg1	techniques					427:436	various instrumental techniques	406:436	various instrumental techniques like FTIR, SEM and EDAX	406:460	The synthesized biocomposites were characterized by various instrumental techniques like FTIR, SEM and EDAX.
27474660	5	57	theme	Dubinin-Radushkevich	799:818	arg1	models					835:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models	774:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models	774:840	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherm models were adopted to examine the adsorption equilibrium.
27474660	7	58	theme	process	1024:1030	arg1	nature					1001:1006	The nature	997:1006	The nature of the sorption process	997:1030	The nature of the sorption process was explained using thermodynamic parameters like ΔS°, ΔG° and ΔH° and a possible mechanism for the sorption of Cr(VI) onto the biocomposites was given.
27474660	4	59	theme	initial	731:737	arg1	concentration					746:758	initial Cr(VI) concentration	731:758	initial Cr(VI) concentration	731:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	59	theme	initial	731:737	arg1	factors					618:624	Various adsorption influencing factors	587:624	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration	587:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	1	60	dep	bi	223:224	arg1	ions					280:283	metal ions	274:283	metal ions	274:283	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	1	60	dep	bi	223:224	arg1	2+					230:231	2+	230:231	2+	230:231	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	1	60	dep	bi	223:224	arg1	Ca					227:228	Ca	227:228	Ca(2+)	227:232	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	4	61	theme	Various	587:593	arg1	concentration					746:758	initial Cr(VI) concentration	731:758	initial Cr(VI) concentration	731:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	61	theme	Various	587:593	arg1	temperature					687:697	temperature	687:697	temperature	687:697	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	61	theme	Various	587:593	arg1	time					640:643	contact time	632:643	contact time	632:643	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	61	theme	Various	587:593	arg1	pH					669:670	pH	669:670	pH of the medium	669:684	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	61	theme	Various	587:593	arg1	presence					700:707	presence	700:707	presence of common co-ions	700:725	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	61	theme	Various	587:593	arg1	factors					618:624	Various adsorption influencing factors	587:624	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration	587:758	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	61	theme	Various	587:593	arg1	dosage					646:651	dosage	646:651	dosage of the sorbent	646:666	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	4	61	theme	Various	587:593	arg1	viz.					626:629	viz.	626:629	viz.	626:629	Various adsorption influencing factors viz., contact time, dosage of the sorbent, pH of the medium, temperature, presence of common co-ions and initial Cr(VI) concentration were studied.
27474660	7	62	theme	sorption	1015:1022	arg1	process					1024:1030	the sorption process	1011:1030	the sorption process	1011:1030	The nature of the sorption process was explained using thermodynamic parameters like ΔS°, ΔG° and ΔH° and a possible mechanism for the sorption of Cr(VI) onto the biocomposites was given.
27474660	3	63	theme	batch	572:576	arg1	process					578:584	batch process	572:584	batch process	572:584	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	64	theme	Ca	517:518	arg1	biocomposites					503:515	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent	499:553	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	3	64	theme	Ca	517:518	arg1	AlgBent					520:526	Ca@AlgBent	517:526	Ca@AlgBent	517:526	Cr(VI) sorption capacities (SCs) of the biocomposites Ca@AlgBent, Ce@AlgBent and Zr@AlgBent were examined by batch process.
27474660	7	65	theme	possible	1105:1112	arg1	mechanism					1114:1122	a possible mechanism	1103:1122	a possible mechanism for the sorption of Cr(VI) onto the biocomposites was given	1103:1182	The nature of the sorption process was explained using thermodynamic parameters like ΔS°, ΔG° and ΔH° and a possible mechanism for the sorption of Cr(VI) onto the biocomposites was given.
27474660	1	66	theme	bentonite	141:149	arg1	clay					158:161	bentonite (Bent) clay	141:161	bentonite (Bent) clay in a biopolymer namely alginate (Alg)	141:199	Biocomposites were synthesized by dispersing bentonite (Bent) clay in a biopolymer namely alginate (Alg) and cross-linked with bi (Ca(2+)), tri (Ce(3+)) and tetravalent (Zr(4+)) metal ions viz., Ca@AlgBent, Ce@AlgBent and Zr@AlgBent composites respectively.
27474660	9	67	theme	biocomposites	1372:1384	arg1	reusability					1353:1363	the reusability	1349:1363	the reusability of the biocomposites	1349:1384	The regeneration studies were carried to know about the reusability of the biocomposites.
27014229	9	0	theme	O-linked	1393:1400	arg1	mannans					1402:1408	C. parapsilosis N- and O-linked mannans	1370:1408	C. parapsilosis N- and O-linked mannans	1370:1408	Therefore, our data are the first to demonstrate that C. parapsilosis N- and O-linked mannans have different roles in host interactions than those reported for C. albicans.
27014229	5	1	theme	morphogenesis	649:661	arg1	failure					638:644	failure	638:644	failure of morphogenesis	638:661	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	5	1	theme	morphogenesis	649:661	arg1	aggregation					625:635	cellular aggregation	616:635	cellular aggregation	616:635	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	5	2	from	wall	696:699	arg1	composition					739:749	wall composition	734:749	wall composition	734:749	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	6	3	link	O-linked	777:784	arg1	mannans					786:792	the cell wall O-linked mannans	763:792	the cell wall O-linked mannans	763:792	We removed the cell wall O-linked mannans by β-elimination, and assessed the relevance of mannans during interaction with human monocytes.
27014229	9	4	link	O-linked	1393:1400	arg1	mannans					1402:1408	C. parapsilosis N- and O-linked mannans	1370:1408	C. parapsilosis N- and O-linked mannans	1370:1408	Therefore, our data are the first to demonstrate that C. parapsilosis N- and O-linked mannans have different roles in host interactions than those reported for C. albicans.
27014229	7	5	theme	IL-6	1090:1093	arg1	stimulation					1095:1105	TNFα and IL-6 stimulation	1081:1105	stimulation	1095:1105	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	6	6	theme	O-linked	777:784	arg1	mannans					786:792	the cell wall O-linked mannans	763:792	the cell wall O-linked mannans	763:792	We removed the cell wall O-linked mannans by β-elimination, and assessed the relevance of mannans during interaction with human monocytes.
27014229	3	7	theme	Golgi	304:308	arg1	α1,6-mannosyltransferase					310:333	a Golgi α1,6-mannosyltransferase	302:333	a Golgi α1,6-mannosyltransferase that plays a key role in the elaboration of the N-linked mannan outer chain	302:409	In Candida albicans, Och1 is a Golgi α1,6-mannosyltransferase that plays a key role in the elaboration of the N-linked mannan outer chain.
27014229	3	7	theme	Golgi	304:308	arg1	Och1					294:297	Och1	294:297	Och1	294:297	In Candida albicans, Och1 is a Golgi α1,6-mannosyltransferase that plays a key role in the elaboration of the N-linked mannan outer chain.
27014229	8	8	theme	lower	1243:1247	arg1	burdens					1256:1262	significantly lower fungal burdens	1229:1262	significantly lower fungal burdens	1229:1262	Furthermore, mice infected with C. parapsilosis och1Δ null mutant cells had significantly lower fungal burdens compared to wild-type (WT)-challenged counterparts.
27014229	7	9	theme	TNFα	1081:1084	arg1	stimulation					1095:1105	TNFα and IL-6 stimulation	1081:1105	stimulation	1095:1105	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	6	10	theme	cell	767:770	arg1	mannans					786:792	the cell wall O-linked mannans	763:792	the cell wall O-linked mannans	763:792	We removed the cell wall O-linked mannans by β-elimination, and assessed the relevance of mannans during interaction with human monocytes.
27014229	9	11	theme	host	1434:1437	arg1	interactions					1439:1450	host interactions	1434:1450	host interactions	1434:1450	Therefore, our data are the first to demonstrate that C. parapsilosis N- and O-linked mannans have different roles in host interactions than those reported for C. albicans.
27014229	8	12	theme	C.	1185:1186	arg1	parapsilosis					1188:1199	C. parapsilosis	1185:1199	C. parapsilosis och1Δ null mutant cells	1185:1223	Furthermore, mice infected with C. parapsilosis och1Δ null mutant cells had significantly lower fungal burdens compared to wild-type (WT)-challenged counterparts.
27014229	4	13	link	N-linked	494:501	arg1	mannosylation					503:515	N-linked mannosylation	494:515	N-linked mannosylation	494:515	Here, we disrupted C. parapsilosis OCH1 to gain insights into the contribution of N-linked mannosylation to cell fitness and to interactions with immune cells.
27014229	2	14	with	points	240:245	arg1	systems					264:270	host immune systems	252:270	host immune systems	252:270	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	4	15	theme	C.	431:432	arg1	OCH1					447:450	C. parapsilosis OCH1	431:450	C. parapsilosis OCH1	431:450	Here, we disrupted C. parapsilosis OCH1 to gain insights into the contribution of N-linked mannosylation to cell fitness and to interactions with immune cells.
27014229	8	16	theme	mutant	1212:1217	arg1	cells					1219:1223	null mutant cells	1207:1223	null mutant cells	1207:1223	Furthermore, mice infected with C. parapsilosis och1Δ null mutant cells had significantly lower fungal burdens compared to wild-type (WT)-challenged counterparts.
27014229	5	17	theme	enhanced	664:671	arg1	susceptibility					673:686	enhanced susceptibility	664:686	enhanced susceptibility to cell wall perturbing agents and defects in wall composition	664:749	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	5	17	theme	enhanced	664:671	arg1	aggregation					625:635	cellular aggregation	616:635	cellular aggregation	616:635	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	3	18	from	α1,6-mannosyltransferase	310:333	arg1	albicans					284:291	Candida albicans	276:291	Candida albicans	276:291	In Candida albicans, Och1 is a Golgi α1,6-mannosyltransferase that plays a key role in the elaboration of the N-linked mannan outer chain.
27014229	2	19	theme	initial	224:230	arg1	points					240:245	initial contact points	224:245	initial contact points with host immune systems	224:270	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	2	19	theme	initial	224:230	arg1	proteins					211:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	7	20	theme	TLR4-dependent	985:998	arg1	pathway					1000:1006	a dectin-1 and TLR4-dependent pathway	970:1006	pathway	1000:1006	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	0	21	theme	Wall	59:62	arg1	Integrity					64:72	Candida parapsilosis Cell Wall Integrity	33:72	Candida parapsilosis Cell Wall Integrity	33:72	Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction.
27014229	3	22	link	N-linked	383:390	arg1	chain					405:409	the N-linked mannan outer chain	379:409	the N-linked mannan outer chain	379:409	In Candida albicans, Och1 is a Golgi α1,6-mannosyltransferase that plays a key role in the elaboration of the N-linked mannan outer chain.
27014229	7	23	theme	dectin-1	972:979	arg1	pathway					1000:1006	a dectin-1 and TLR4-dependent pathway	970:1006	pathway	1000:1006	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	1	24	theme	opportunistic	143:155	arg1	parapsilosis					104:115	Candida parapsilosis	96:115	Candida parapsilosis	96:115	Candida parapsilosis is an important, emerging opportunistic fungal pathogen.
27014229	1	24	theme	opportunistic	143:155	arg1	pathogen					164:171	emerging opportunistic fungal pathogen	134:171	emerging opportunistic fungal pathogen	134:171	Candida parapsilosis is an important, emerging opportunistic fungal pathogen.
27014229	5	25	theme	Och1	580:583	arg1	parapsilosis					591:602	Och1 in C. parapsilosis	580:602	Och1 in C. parapsilosis	580:602	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	0	26	from	Role	0:3	arg1	Integrity					64:72	Candida parapsilosis Cell Wall Integrity	33:72	Candida parapsilosis Cell Wall Integrity	33:72	Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction.
27014229	0	26	from	Role	0:3	arg1	Interaction					83:93	Host Interaction	78:93	Host Interaction	78:93	Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction.
27014229	7	27	theme	IL-1β	949:953	arg1	stimulation					955:965	IL-1β stimulation	949:965	IL-1β stimulation in a dectin-1 and TLR4-dependent pathway	949:1006	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	3	28	theme	key	348:350	arg1	role					352:355	a key role	346:355	a key role	346:355	In Candida albicans, Och1 is a Golgi α1,6-mannosyltransferase that plays a key role in the elaboration of the N-linked mannan outer chain.
27014229	5	29	theme	wall	734:737	arg1	composition					739:749	wall composition	734:749	wall composition	734:749	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	0	30	theme	Protein	8:14	arg1	Glycosylation					16:28	Protein Glycosylation	8:28	Protein Glycosylation	8:28	Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction.
27014229	3	31	theme	chain	405:409	arg1	elaboration					364:374	the elaboration	360:374	the elaboration of the N-linked mannan outer chain	360:409	In Candida albicans, Och1 is a Golgi α1,6-mannosyltransferase that plays a key role in the elaboration of the N-linked mannan outer chain.
27014229	2	32	theme	mannosylated	181:192	arg1	points					240:245	initial contact points	224:245	initial contact points with host immune systems	224:270	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	2	32	theme	mannosylated	181:192	arg1	proteins					211:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	7	33	theme	IL-10	1135:1139	arg1	production					1141:1150	IL-10 production	1135:1150	IL-10 production	1135:1150	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	6	34	theme	mannans	842:848	arg1	relevance					829:837	the relevance	825:837	the relevance of mannans	825:848	We removed the cell wall O-linked mannans by β-elimination, and assessed the relevance of mannans during interaction with human monocytes.
27014229	3	35	theme	mannan	392:397	arg1	chain					405:409	the N-linked mannan outer chain	379:409	the N-linked mannan outer chain	379:409	In Candida albicans, Och1 is a Golgi α1,6-mannosyltransferase that plays a key role in the elaboration of the N-linked mannan outer chain.
27014229	0	36	theme	parapsilosis	41:52	arg1	Integrity					64:72	Candida parapsilosis Cell Wall Integrity	33:72	Candida parapsilosis Cell Wall Integrity	33:72	Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction.
27014229	2	37	theme	host	252:255	arg1	systems					264:270	host immune systems	252:270	host immune systems	252:270	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	9	38	contain	have	1410:1413	arg1	mannans					1402:1408	C. parapsilosis N- and O-linked mannans	1370:1408	C. parapsilosis N- and O-linked mannans	1370:1408	Therefore, our data are the first to demonstrate that C. parapsilosis N- and O-linked mannans have different roles in host interactions than those reported for C. albicans.
27014229	9	38	contain	have	1410:1413	arg2	roles					1425:1429	different roles	1415:1429	different roles	1415:1429	Therefore, our data are the first to demonstrate that C. parapsilosis N- and O-linked mannans have different roles in host interactions than those reported for C. albicans.
27014229	7	39	link	O-linked	1030:1037	arg1	mannans					1039:1045	N- and O-linked mannans	1023:1045	N- and O-linked mannans	1023:1045	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	7	39	link	O-linked	1030:1037	arg1	ligands					1069:1075	equally important ligands	1051:1075	equally important ligands for TNFα and IL-6 stimulation	1051:1105	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	7	40	theme	O-linked	914:921	arg1	mannans					923:929	O-linked mannans	914:929	O-linked mannans	914:929	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	7	41	from	stimulation	955:965	arg1	pathway					1000:1006	a dectin-1 and TLR4-dependent pathway	970:1006	pathway	1000:1006	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	7	42	link	O-linked	914:921	arg1	mannans					923:929	O-linked mannans	914:929	O-linked mannans	914:929	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	8	43	dep	wild-type	1276:1284	arg1	WT					1287:1288	WT	1287:1288	WT	1287:1288	Furthermore, mice infected with C. parapsilosis och1Δ null mutant cells had significantly lower fungal burdens compared to wild-type (WT)-challenged counterparts.
27014229	4	44	with	interactions	540:551	arg1	cells					565:569	immune cells	558:569	immune cells	558:569	Here, we disrupted C. parapsilosis OCH1 to gain insights into the contribution of N-linked mannosylation to cell fitness and to interactions with immune cells.
27014229	9	45	theme	different	1415:1423	arg1	roles					1425:1429	different roles	1415:1429	different roles	1415:1429	Therefore, our data are the first to demonstrate that C. parapsilosis N- and O-linked mannans have different roles in host interactions than those reported for C. albicans.
27014229	6	46	theme	human	874:878	arg1	monocytes					880:888	human monocytes	874:888	human monocytes	874:888	We removed the cell wall O-linked mannans by β-elimination, and assessed the relevance of mannans during interaction with human monocytes.
27014229	5	47	theme	cellular	616:623	arg1	susceptibility					673:686	enhanced susceptibility	664:686	enhanced susceptibility to cell wall perturbing agents and defects in wall composition	664:749	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	5	47	theme	cellular	616:623	arg1	failure					638:644	failure	638:644	failure of morphogenesis	638:661	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	5	47	theme	cellular	616:623	arg1	aggregation					625:635	cellular aggregation	616:635	cellular aggregation	616:635	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	6	48	theme	wall	772:775	arg1	mannans					786:792	the cell wall O-linked mannans	763:792	the cell wall O-linked mannans	763:792	We removed the cell wall O-linked mannans by β-elimination, and assessed the relevance of mannans during interaction with human monocytes.
27014229	8	49	contain	had	1225:1227	arg1	mice					1166:1169	mice	1166:1169	mice infected with C. parapsilosis och1Δ null mutant cells	1166:1223	Furthermore, mice infected with C. parapsilosis och1Δ null mutant cells had significantly lower fungal burdens compared to wild-type (WT)-challenged counterparts.
27014229	8	49	contain	had	1225:1227	arg2	burdens					1256:1262	significantly lower fungal burdens	1229:1262	significantly lower fungal burdens	1229:1262	Furthermore, mice infected with C. parapsilosis och1Δ null mutant cells had significantly lower fungal burdens compared to wild-type (WT)-challenged counterparts.
27014229	7	50	theme	important	1059:1067	arg1	mannans					1039:1045	N- and O-linked mannans	1023:1045	N- and O-linked mannans	1023:1045	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	7	50	theme	important	1059:1067	arg1	ligands					1069:1075	equally important ligands	1051:1075	equally important ligands for TNFα and IL-6 stimulation	1051:1105	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	4	51	theme	parapsilosis	434:445	arg1	OCH1					447:450	C. parapsilosis OCH1	431:450	C. parapsilosis OCH1	431:450	Here, we disrupted C. parapsilosis OCH1 to gain insights into the contribution of N-linked mannosylation to cell fitness and to interactions with immune cells.
27014229	8	52	theme	fungal	1249:1254	arg1	burdens					1256:1262	significantly lower fungal burdens	1229:1262	significantly lower fungal burdens	1229:1262	Furthermore, mice infected with C. parapsilosis och1Δ null mutant cells had significantly lower fungal burdens compared to wild-type (WT)-challenged counterparts.
27014229	5	53	theme	perturbing	701:710	arg1	agents					712:717	perturbing agents	701:717	perturbing agents	701:717	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	3	54	theme	Candida	276:282	arg1	albicans					284:291	Candida albicans	276:291	Candida albicans	276:291	In Candida albicans, Och1 is a Golgi α1,6-mannosyltransferase that plays a key role in the elaboration of the N-linked mannan outer chain.
27014229	7	55	theme	O-linked	1030:1037	arg1	mannans					1039:1045	N- and O-linked mannans	1023:1045	N- and O-linked mannans	1023:1045	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	7	55	theme	O-linked	1030:1037	arg1	ligands					1069:1075	equally important ligands	1051:1075	equally important ligands for TNFα and IL-6 stimulation	1051:1105	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	5	56	from	defects	723:729	arg1	composition					739:749	wall composition	734:749	wall composition	734:749	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	2	57	theme	contact	232:238	arg1	points					240:245	initial contact points	224:245	initial contact points with host immune systems	224:270	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	2	57	theme	contact	232:238	arg1	proteins					211:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	8	58	theme	null	1207:1210	arg1	cells					1219:1223	null mutant cells	1207:1223	null mutant cells	1207:1223	Furthermore, mice infected with C. parapsilosis och1Δ null mutant cells had significantly lower fungal burdens compared to wild-type (WT)-challenged counterparts.
27014229	5	59	theme	cell	691:694	arg1	wall					696:699	cell wall perturbing agents and defects in wall composition	691:749	wall	696:699	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	8	60	theme	wild-type	1276:1284	arg1	counterparts					1302:1313	wild-type (WT)-challenged counterparts	1276:1313	wild-type (WT)-challenged counterparts	1276:1313	Furthermore, mice infected with C. parapsilosis och1Δ null mutant cells had significantly lower fungal burdens compared to wild-type (WT)-challenged counterparts.
27014229	7	61	theme	N-	1023:1024	arg1	mannans					1039:1045	N- and O-linked mannans	1023:1045	N- and O-linked mannans	1023:1045	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	7	61	theme	N-	1023:1024	arg1	ligands					1069:1075	equally important ligands	1051:1075	equally important ligands for TNFα and IL-6 stimulation	1051:1105	Results indicated that O-linked mannans are important for IL-1β stimulation in a dectin-1 and TLR4-dependent pathway; whereas both, N- and O-linked mannans are equally important ligands for TNFα and IL-6 stimulation, but neither is involved in IL-10 production.
27014229	1	62	theme	important	123:131	arg1	parapsilosis					104:115	Candida parapsilosis	96:115	Candida parapsilosis	96:115	Candida parapsilosis is an important, emerging opportunistic fungal pathogen.
27014229	1	62	theme	important	123:131	arg1	pathogen					164:171	emerging opportunistic fungal pathogen	134:171	emerging opportunistic fungal pathogen	134:171	Candida parapsilosis is an important, emerging opportunistic fungal pathogen.
27014229	2	63	theme	immune	257:262	arg1	systems					264:270	host immune systems	252:270	host immune systems	252:270	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	1	64	theme	emerging	134:141	arg1	parapsilosis					104:115	Candida parapsilosis	96:115	Candida parapsilosis	96:115	Candida parapsilosis is an important, emerging opportunistic fungal pathogen.
27014229	1	64	theme	emerging	134:141	arg1	pathogen					164:171	emerging opportunistic fungal pathogen	134:171	emerging opportunistic fungal pathogen	134:171	Candida parapsilosis is an important, emerging opportunistic fungal pathogen.
27014229	8	65	theme	-challenged	1290:1300	arg1	counterparts					1302:1313	wild-type (WT)-challenged counterparts	1276:1313	wild-type (WT)-challenged counterparts	1276:1313	Furthermore, mice infected with C. parapsilosis och1Δ null mutant cells had significantly lower fungal burdens compared to wild-type (WT)-challenged counterparts.
27014229	2	66	gly	mannosylated	181:192	arg1	points					240:245	initial contact points	224:245	initial contact points with host immune systems	224:270	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	2	66	gly	mannosylated	181:192	arg1	proteins					211:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	0	67	theme	Host	78:81	arg1	Interaction					83:93	Host Interaction	78:93	Host Interaction	78:93	Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction.
27014229	1	68	theme	fungal	157:162	arg1	parapsilosis					104:115	Candida parapsilosis	96:115	Candida parapsilosis	96:115	Candida parapsilosis is an important, emerging opportunistic fungal pathogen.
27014229	1	68	theme	fungal	157:162	arg1	pathogen					164:171	emerging opportunistic fungal pathogen	134:171	emerging opportunistic fungal pathogen	134:171	Candida parapsilosis is an important, emerging opportunistic fungal pathogen.
27014229	5	69	dep	wall	696:699	arg1	agents					712:717	perturbing agents	701:717	perturbing agents	701:717	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	4	70	theme	mannosylation	503:515	arg1	contribution					478:489	the contribution	474:489	the contribution of N-linked mannosylation to cell fitness and to interactions with immune cells	474:569	Here, we disrupted C. parapsilosis OCH1 to gain insights into the contribution of N-linked mannosylation to cell fitness and to interactions with immune cells.
27014229	2	71	theme	wall	206:209	arg1	points					240:245	initial contact points	224:245	initial contact points with host immune systems	224:270	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	2	71	theme	wall	206:209	arg1	proteins					211:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	5	72	theme	in	585:586	arg1	parapsilosis					591:602	Och1 in C. parapsilosis	580:602	Och1 in C. parapsilosis	580:602	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	5	73	theme	parapsilosis	591:602	arg1	Loss					572:575	Loss	572:575	Loss of Och1 in C. parapsilosis	572:602	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	6	74	with	interaction	857:867	arg1	monocytes					880:888	human monocytes	874:888	human monocytes	874:888	We removed the cell wall O-linked mannans by β-elimination, and assessed the relevance of mannans during interaction with human monocytes.
27014229	3	75	theme	N-linked	383:390	arg1	chain					405:409	the N-linked mannan outer chain	379:409	the N-linked mannan outer chain	379:409	In Candida albicans, Och1 is a Golgi α1,6-mannosyltransferase that plays a key role in the elaboration of the N-linked mannan outer chain.
27014229	2	76	theme	cell	201:204	arg1	points					240:245	initial contact points	224:245	initial contact points with host immune systems	224:270	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	2	76	theme	cell	201:204	arg1	proteins					211:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	9	77	dep	N-	1386:1387	arg1	parapsilosis					1373:1384	parapsilosis	1373:1384	parapsilosis	1373:1384	Therefore, our data are the first to demonstrate that C. parapsilosis N- and O-linked mannans have different roles in host interactions than those reported for C. albicans.
27014229	9	77	dep	N-	1386:1387	arg1	C.					1370:1371	C.	1370:1371	C.	1370:1371	Therefore, our data are the first to demonstrate that C. parapsilosis N- and O-linked mannans have different roles in host interactions than those reported for C. albicans.
27014229	0	78	theme	Glycosylation	16:28	arg1	Role					0:3	Role	0:3	Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction.	0:94	Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction.
27014229	1	79	theme	Candida	96:102	arg1	pathogen					164:171	emerging opportunistic fungal pathogen	134:171	emerging opportunistic fungal pathogen	134:171	Candida parapsilosis is an important, emerging opportunistic fungal pathogen.
27014229	1	79	theme	Candida	96:102	arg1	parapsilosis					104:115	Candida parapsilosis	96:115	Candida parapsilosis	96:115	Candida parapsilosis is an important, emerging opportunistic fungal pathogen.
27014229	4	80	theme	immune	558:563	arg1	cells					565:569	immune cells	558:569	immune cells	558:569	Here, we disrupted C. parapsilosis OCH1 to gain insights into the contribution of N-linked mannosylation to cell fitness and to interactions with immune cells.
27014229	2	81	theme	fungal	194:199	arg1	points					240:245	initial contact points	224:245	initial contact points with host immune systems	224:270	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	2	81	theme	fungal	194:199	arg1	proteins					211:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins	174:218	Highly mannosylated fungal cell wall proteins are initial contact points with host immune systems.
27014229	0	82	theme	Candida	33:39	arg1	Integrity					64:72	Candida parapsilosis Cell Wall Integrity	33:72	Candida parapsilosis Cell Wall Integrity	33:72	Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction.
27014229	4	83	theme	cell	520:523	arg1	fitness					525:531	cell fitness	520:531	cell fitness	520:531	Here, we disrupted C. parapsilosis OCH1 to gain insights into the contribution of N-linked mannosylation to cell fitness and to interactions with immune cells.
27014229	0	84	theme	Cell	54:57	arg1	Integrity					64:72	Candida parapsilosis Cell Wall Integrity	33:72	Candida parapsilosis Cell Wall Integrity	33:72	Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction.
27014229	3	85	theme	outer	399:403	arg1	chain					405:409	the N-linked mannan outer chain	379:409	the N-linked mannan outer chain	379:409	In Candida albicans, Och1 is a Golgi α1,6-mannosyltransferase that plays a key role in the elaboration of the N-linked mannan outer chain.
27014229	4	86	theme	N-linked	494:501	arg1	mannosylation					503:515	N-linked mannosylation	494:515	N-linked mannosylation	494:515	Here, we disrupted C. parapsilosis OCH1 to gain insights into the contribution of N-linked mannosylation to cell fitness and to interactions with immune cells.
27014229	5	87	dep	in	585:586	arg1	C.					588:589	C.	588:589	C.	588:589	Loss of Och1 in C. parapsilosis resulted in cellular aggregation, failure of morphogenesis, enhanced susceptibility to cell wall perturbing agents and defects in wall composition.
27014229	9	88	theme	N-	1386:1387	arg1	mannans					1402:1408	C. parapsilosis N- and O-linked mannans	1370:1408	C. parapsilosis N- and O-linked mannans	1370:1408	Therefore, our data are the first to demonstrate that C. parapsilosis N- and O-linked mannans have different roles in host interactions than those reported for C. albicans.
26004575	2	0	theme	lipophilic	397:406	arg1	molecule					430:437	lipophilic and fluorescent model molecule	397:437	lipophilic and fluorescent model molecule	397:437	Zinc(II) phthalocyanine (ZnPc), selected as lipophilic and fluorescent model molecule, was loaded inside NPs by a melting/sonication procedure.
26004575	8	1	with	line	1421:1424	arg1	dosage					1436:1441	ZnPc dosage	1431:1441	ZnPc dosage in the skin	1431:1453	Fluorescence images of skin samples after 24h of permeation were in line with ZnPc dosage in the skin and demonstrated the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces.
26004575	8	2	theme	ZnPc	1431:1434	arg1	dosage					1436:1441	ZnPc dosage	1431:1441	ZnPc dosage in the skin	1431:1453	Fluorescence images of skin samples after 24h of permeation were in line with ZnPc dosage in the skin and demonstrated the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces.
26004575	7	3	theme	other	1162:1166	arg1	hand					1168:1171	the other hand	1158:1171	the other hand	1158:1171	On the other hand, ZnPc was found in the skin in large amount and also in the viable epidermis when delivered through NPs associated with HPβCD, especially in conditions limiting water evaporation.
26004575	9	4	theme	confocal	1654:1661	arg1	spectroscopy					1669:1680	confocal Raman spectroscopy	1654:1680	confocal Raman spectroscopy	1654:1680	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	1	5	dep	-poly	223:227	arg1	copolymer					265:273	-amphiphilic diblock copolymer	244:273	a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer	200:273	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	9	6	theme	water	1730:1734	arg1	profile					1736:1742	water profile	1730:1742	water profile	1730:1742	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	5	7	theme	Franz-type	836:845	arg1	cells					857:861	Franz-type diffusion cells	836:861	Franz-type diffusion cells	836:861	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	5	8	contain	containing	925:934	arg1	vehicles					894:901	different vehicles	884:901	different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer	884:1044	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	5	8	contain	containing	925:934	arg2	NPs					961:963	ZnPc-loaded NPs	949:963	ZnPc-loaded NPs	949:963	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	5	8	contain	containing	925:934	arg2	ZnPc					941:944	free ZnPc	936:944	free ZnPc	936:944	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	5	8	contain	containing	925:934	arg1	0.4kDa					917:922	water or PEG 0.4kDa	904:922	0.4kDa	917:922	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	1	9	theme	ethylene	207:214	arg1	-poly					223:227	a poly(ethylene glycol)-poly	200:227	a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer	200:273	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	1	9	theme	ethylene	207:214	arg1	glycol					216:221	ethylene glycol	207:221	ethylene glycol	207:221	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	9	10	theme	Raman	1663:1667	arg1	spectroscopy					1669:1680	confocal Raman spectroscopy	1654:1680	confocal Raman spectroscopy	1654:1680	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	5	11	theme	water	904:908	arg1	vehicles					894:901	different vehicles	884:901	different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer	884:1044	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	5	11	theme	water	904:908	arg1	0.4kDa					917:922	water or PEG 0.4kDa	904:922	0.4kDa	917:922	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	8	12	theme	intercellular	1567:1579	arg1	spaces					1581:1586	the intercellular spaces	1563:1586	the intercellular spaces	1563:1586	Fluorescence images of skin samples after 24h of permeation were in line with ZnPc dosage in the skin and demonstrated the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces.
26004575	0	13	theme	2-hydroxypropyl	76:90	arg1	-β-cyclodextrin					92:106	(2-hydroxypropyl)-β-cyclodextrin	75:106	(2-hydroxypropyl)-β-cyclodextrin	75:106	Skin transport of PEGylated poly(ε-caprolactone) nanoparticles assisted by (2-hydroxypropyl)-β-cyclodextrin.
26004575	3	14	theme	ZnPc	591:594	arg1	entrapment					596:605	a ZnPc entrapment	589:605	a ZnPc entrapment dependent on polymer/ZnPc ratio	589:637	Loaded NPs with a hydrodynamic diameter around 60nm, a slightly negative zeta potential and a ZnPc entrapment dependent on polymer/ZnPc ratio were obtained.
26004575	9	15	theme	NP	1861:1862	arg1	transport					1864:1872	NP transport	1861:1872	NP transport	1861:1872	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	9	16	theme	hydration	1787:1795	arg1	degree					1777:1782	the degree	1773:1782	the degree of hydration	1773:1795	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	9	17	from	alteration	1716:1725	arg1	skin					1751:1754	the skin	1747:1754	the skin	1747:1754	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	8	18	dep	ability	1476:1482	arg1	locate					1553:1558	locate	1553:1558	to locate in the intercellular spaces	1550:1586	Fluorescence images of skin samples after 24h of permeation were in line with ZnPc dosage in the skin and demonstrated the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces.
26004575	8	18	dep	ability	1476:1482	arg1	penetrate					1527:1535	penetrate	1527:1535	to penetrate the skin	1524:1544	Fluorescence images of skin samples after 24h of permeation were in line with ZnPc dosage in the skin and demonstrated the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces.
26004575	5	19	theme	PEG	913:915	arg1	vehicles					894:901	different vehicles	884:901	different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer	884:1044	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	5	19	theme	PEG	913:915	arg1	0.4kDa					917:922	water or PEG 0.4kDa	904:922	0.4kDa	917:922	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	7	20	theme	viable	1233:1238	arg1	epidermis					1240:1248	the viable epidermis	1229:1248	the viable epidermis	1229:1248	On the other hand, ZnPc was found in the skin in large amount and also in the viable epidermis when delivered through NPs associated with HPβCD, especially in conditions limiting water evaporation.
26004575	2	21	theme	model	424:428	arg1	molecule					430:437	lipophilic and fluorescent model molecule	397:437	lipophilic and fluorescent model molecule	397:437	Zinc(II) phthalocyanine (ZnPc), selected as lipophilic and fluorescent model molecule, was loaded inside NPs by a melting/sonication procedure.
26004575	3	22	theme	polymer/ZnPc	620:631	arg1	ratio					633:637	polymer/ZnPc ratio	620:637	polymer/ZnPc ratio	620:637	Loaded NPs with a hydrodynamic diameter around 60nm, a slightly negative zeta potential and a ZnPc entrapment dependent on polymer/ZnPc ratio were obtained.
26004575	5	23	with	treatment	869:877	arg1	vehicles					894:901	different vehicles	884:901	different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer	884:1044	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	5	23	with	treatment	869:877	arg1	0.4kDa					917:922	water or PEG 0.4kDa	904:922	0.4kDa	917:922	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	10	24	theme	PEG-b-PCL	1915:1923	arg1	vehicle					1959:1965	a novel vehicle	1951:1965	a novel vehicle for the skin delivery of highly lipophilic compounds paving the way to several applications	1951:2057	Taken together, these results highlight PEG-b-PCL NPs coupled with HPβCD as a novel vehicle for the skin delivery of highly lipophilic compounds paving the way to several applications.
26004575	10	24	theme	PEG-b-PCL	1915:1923	arg1	NPs					1925:1927	PEG-b-PCL NPs	1915:1927	PEG-b-PCL NPs coupled with HPβCD	1915:1946	Taken together, these results highlight PEG-b-PCL NPs coupled with HPβCD as a novel vehicle for the skin delivery of highly lipophilic compounds paving the way to several applications.
26004575	9	25	theme	stratum	1800:1806	arg1	interface					1833:1841	stratum corneum/viable epidermis interface	1800:1841	stratum corneum/viable epidermis interface which can promote NP transport	1800:1872	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	5	26	theme	porcine	802:808	arg1	skin					814:817	porcine ear skin	802:817	porcine ear skin	802:817	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	1	27	theme	drug	315:318	arg1	system					328:333	drug carrier system	315:333	drug carrier system	315:333	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	4	28	theme	Spectroscopic	654:666	arg1	investigations					668:681	Spectroscopic investigations	654:681	Spectroscopic investigations	654:681	Spectroscopic investigations evidenced that ZnPc was entrapped in monomeric form maintaining its emission properties.
26004575	9	29	theme	corneum/viable	1808:1821	arg1	interface					1833:1841	stratum corneum/viable epidermis interface	1800:1841	stratum corneum/viable epidermis interface which can promote NP transport	1800:1872	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	5	30	theme	ear	810:812	arg1	skin					814:817	porcine ear skin	802:817	porcine ear skin	802:817	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	0	31	theme	Skin	0:3	arg1	transport					5:13	Skin transport	0:13	Skin transport of PEGylated poly(ε-caprolactone)	0:47	Skin transport of PEGylated poly(ε-caprolactone) nanoparticles assisted by (2-hydroxypropyl)-β-cyclodextrin.
26004575	10	32	theme	several	2038:2044	arg1	applications					2046:2057	several applications	2038:2057	several applications	2038:2057	Taken together, these results highlight PEG-b-PCL NPs coupled with HPβCD as a novel vehicle for the skin delivery of highly lipophilic compounds paving the way to several applications.
26004575	1	33	dep	investigate	137:147	arg1	PEG-b-PCL					276:284	PEG-b-PCL	276:284	PEG-b-PCL	276:284	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	9	34	theme	epidermis	1823:1831	arg1	interface					1833:1841	stratum corneum/viable epidermis interface	1800:1841	stratum corneum/viable epidermis interface which can promote NP transport	1800:1872	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	1	35	theme	small	166:170	arg1	NPs					187:189	NPs	187:189	NPs	187:189	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	1	35	theme	small	166:170	arg1	nanoparticles					172:184	small nanoparticles	166:184	small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer	166:273	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	0	36	theme	PEGylated	18:26	arg1	ε-caprolactone					33:46	ε-caprolactone	33:46	ε-caprolactone	33:46	Skin transport of PEGylated poly(ε-caprolactone) nanoparticles assisted by (2-hydroxypropyl)-β-cyclodextrin.
26004575	0	36	theme	PEGylated	18:26	arg1	poly					28:31	PEGylated poly	18:31	PEGylated poly(ε-caprolactone)	18:47	Skin transport of PEGylated poly(ε-caprolactone) nanoparticles assisted by (2-hydroxypropyl)-β-cyclodextrin.
26004575	5	37	theme	free	936:939	arg1	ZnPc					941:944	free ZnPc	936:944	free ZnPc	936:944	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	1	38	theme	nanoparticles	172:184	arg1	potential					153:161	the potential	149:161	the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer	149:273	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	5	39	theme	different	884:892	arg1	vehicles					894:901	different vehicles	884:901	different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer	884:1044	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	5	39	theme	different	884:892	arg1	0.4kDa					917:922	water or PEG 0.4kDa	904:922	0.4kDa	917:922	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	1	40	dep	PEG-b-PCL	276:284	arg1	PEG=2kDa					287:294	PEG=2kDa	287:294	PEG=2kDa	287:294	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	1	40	dep	PEG-b-PCL	276:284	arg1	PCL=4.2kDa					300:309	PCL=4.2kDa	300:309	PCL=4.2kDa	300:309	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	10	41	theme	lipophilic	1999:2008	arg1	compounds					2010:2018	highly lipophilic compounds	1992:2018	highly lipophilic compounds paving the way to several applications	1992:2057	Taken together, these results highlight PEG-b-PCL NPs coupled with HPβCD as a novel vehicle for the skin delivery of highly lipophilic compounds paving the way to several applications.
26004575	1	42	theme	carrier	320:326	arg1	system					328:333	drug carrier system	315:333	drug carrier system	315:333	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	7	43	located	found	1183:1187	arg1	skin					1196:1199	the skin	1192:1199	the skin	1192:1199	On the other hand, ZnPc was found in the skin in large amount and also in the viable epidermis when delivered through NPs associated with HPβCD, especially in conditions limiting water evaporation.
26004575	7	43	located	found	1183:1187	arg1	amount					1210:1215	large amount	1204:1215	large amount	1204:1215	On the other hand, ZnPc was found in the skin in large amount and also in the viable epidermis when delivered through NPs associated with HPβCD, especially in conditions limiting water evaporation.
26004575	7	43	located	found	1183:1187	arg2	ZnPc					1174:1177	ZnPc	1174:1177	ZnPc	1174:1177	On the other hand, ZnPc was found in the skin in large amount and also in the viable epidermis when delivered through NPs associated with HPβCD, especially in conditions limiting water evaporation.
26004575	7	43	located	found	1183:1187	arg1	hand					1168:1171	the other hand	1158:1171	the other hand	1158:1171	On the other hand, ZnPc was found in the skin in large amount and also in the viable epidermis when delivered through NPs associated with HPβCD, especially in conditions limiting water evaporation.
26004575	7	43	located	found	1183:1187	arg1	conditions					1314:1323	conditions	1314:1323	conditions limiting water evaporation	1314:1350	On the other hand, ZnPc was found in the skin in large amount and also in the viable epidermis when delivered through NPs associated with HPβCD, especially in conditions limiting water evaporation.
26004575	2	44	theme	Zinc	353:356	arg1	phthalocyanine					362:375	Zinc(II) phthalocyanine	353:375	Zinc(II) phthalocyanine (ZnPc)	353:382	Zinc(II) phthalocyanine (ZnPc), selected as lipophilic and fluorescent model molecule, was loaded inside NPs by a melting/sonication procedure.
26004575	2	44	theme	Zinc	353:356	arg1	ZnPc					378:381	ZnPc	378:381	ZnPc	378:381	Zinc(II) phthalocyanine (ZnPc), selected as lipophilic and fluorescent model molecule, was loaded inside NPs by a melting/sonication procedure.
26004575	8	45	from	dosage	1436:1441	arg1	skin					1450:1453	the skin	1446:1453	the skin	1446:1453	Fluorescence images of skin samples after 24h of permeation were in line with ZnPc dosage in the skin and demonstrated the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces.
26004575	10	46	theme	compounds	2010:2018	arg1	delivery					1980:1987	the skin delivery	1971:1987	the skin delivery of highly lipophilic compounds paving the way to several applications	1971:2057	Taken together, these results highlight PEG-b-PCL NPs coupled with HPβCD as a novel vehicle for the skin delivery of highly lipophilic compounds paving the way to several applications.
26004575	3	47	theme	negative	561:568	arg1	potential					575:583	a slightly negative zeta potential	550:583	a slightly negative zeta potential	550:583	Loaded NPs with a hydrodynamic diameter around 60nm, a slightly negative zeta potential and a ZnPc entrapment dependent on polymer/ZnPc ratio were obtained.
26004575	4	48	theme	emission	751:758	arg1	properties					760:769	its emission properties	747:769	its emission properties	747:769	Spectroscopic investigations evidenced that ZnPc was entrapped in monomeric form maintaining its emission properties.
26004575	0	49	theme	poly	28:31	arg1	transport					5:13	Skin transport	0:13	Skin transport of PEGylated poly(ε-caprolactone)	0:47	Skin transport of PEGylated poly(ε-caprolactone) nanoparticles assisted by (2-hydroxypropyl)-β-cyclodextrin.
26004575	1	50	theme	-amphiphilic	244:255	arg1	copolymer					265:273	-amphiphilic diblock copolymer	244:273	a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer	200:273	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	9	51	theme	skin	1602:1605	arg1	properties					1616:1625	skin chemical properties	1602:1625	skin chemical properties upon application of NPs by confocal Raman spectroscopy	1602:1680	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	10	52	theme	skin	1975:1978	arg1	delivery					1980:1987	the skin delivery	1971:1987	the skin delivery of highly lipophilic compounds paving the way to several applications	1971:2057	Taken together, these results highlight PEG-b-PCL NPs coupled with HPβCD as a novel vehicle for the skin delivery of highly lipophilic compounds paving the way to several applications.
26004575	5	53	theme	ZnPc-loaded	949:959	arg1	NPs					961:963	ZnPc-loaded NPs	949:963	ZnPc-loaded NPs	949:963	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	8	54	theme	Fluorescence	1353:1364	arg1	images					1366:1371	Fluorescence images	1353:1371	Fluorescence images of skin samples after 24h of permeation	1353:1411	Fluorescence images of skin samples after 24h of permeation were in line with ZnPc dosage in the skin and demonstrated the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces.
26004575	5	55	theme	ZnPc	789:792	arg1	transport					776:784	The transport	772:784	The transport of ZnPc through porcine ear skin	772:817	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	9	56	theme	profile	1736:1742	arg1	alteration					1716:1725	an alteration	1713:1725	an alteration of water profile in the skin	1713:1754	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	1	57	theme	diblock	257:263	arg1	copolymer					265:273	-amphiphilic diblock copolymer	244:273	a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer	200:273	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	9	58	theme	chemical	1607:1614	arg1	properties					1616:1625	skin chemical properties	1602:1625	skin chemical properties upon application of NPs by confocal Raman spectroscopy	1602:1680	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	3	59	theme	Loaded	497:502	arg1	NPs					504:506	Loaded NPs	497:506	Loaded NPs with a hydrodynamic diameter around 60nm, a slightly negative zeta potential and a ZnPc entrapment dependent on polymer/ZnPc ratio	497:637	Loaded NPs with a hydrodynamic diameter around 60nm, a slightly negative zeta potential and a ZnPc entrapment dependent on polymer/ZnPc ratio were obtained.
26004575	5	60	theme	permeation	1026:1035	arg1	enhancer					1037:1044	permeation enhancer	1026:1044	permeation enhancer	1026:1044	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	5	61	theme	diffusion	847:855	arg1	cells					857:861	Franz-type diffusion cells	836:861	Franz-type diffusion cells	836:861	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	8	62	theme	permeation	1402:1411	arg1	24h					1395:1397	24h	1395:1397	24h of permeation	1395:1411	Fluorescence images of skin samples after 24h of permeation were in line with ZnPc dosage in the skin and demonstrated the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces.
26004575	8	63	theme	samples	1381:1387	arg1	images					1366:1371	Fluorescence images	1353:1371	Fluorescence images of skin samples after 24h of permeation	1353:1411	Fluorescence images of skin samples after 24h of permeation were in line with ZnPc dosage in the skin and demonstrated the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces.
26004575	8	64	theme	skin	1376:1379	arg1	samples					1381:1387	skin samples	1376:1387	skin samples after 24h of permeation	1376:1411	Fluorescence images of skin samples after 24h of permeation were in line with ZnPc dosage in the skin and demonstrated the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces.
26004575	3	65	with	NPs	504:506	arg1	diameter					528:535	a hydrodynamic diameter	513:535	a hydrodynamic diameter around 60nm, a slightly negative zeta potential and a ZnPc entrapment dependent on polymer/ZnPc ratio	513:637	Loaded NPs with a hydrodynamic diameter around 60nm, a slightly negative zeta potential and a ZnPc entrapment dependent on polymer/ZnPc ratio were obtained.
26004575	2	66	theme	melting/sonication	467:484	arg1	procedure					486:494	a melting/sonication procedure	465:494	a melting/sonication procedure	465:494	Zinc(II) phthalocyanine (ZnPc), selected as lipophilic and fluorescent model molecule, was loaded inside NPs by a melting/sonication procedure.
26004575	6	67	theme	receptor	1133:1140	arg1	compartment					1142:1152	receptor compartment	1133:1152	receptor compartment	1133:1152	Independently of the sample tested, ZnPc was transported in the skin without reaching receptor compartment.
26004575	4	68	theme	monomeric	720:728	arg1	form					730:733	monomeric form	720:733	monomeric form maintaining its emission properties	720:769	Spectroscopic investigations evidenced that ZnPc was entrapped in monomeric form maintaining its emission properties.
26004575	3	69	theme	zeta	570:573	arg1	potential					575:583	a slightly negative zeta potential	550:583	a slightly negative zeta potential	550:583	Loaded NPs with a hydrodynamic diameter around 60nm, a slightly negative zeta potential and a ZnPc entrapment dependent on polymer/ZnPc ratio were obtained.
26004575	7	70	theme	water	1334:1338	arg1	evaporation					1340:1350	water evaporation	1334:1350	water evaporation	1334:1350	On the other hand, ZnPc was found in the skin in large amount and also in the viable epidermis when delivered through NPs associated with HPβCD, especially in conditions limiting water evaporation.
26004575	10	71	theme	novel	1953:1957	arg1	vehicle					1959:1965	a novel vehicle	1951:1965	a novel vehicle for the skin delivery of highly lipophilic compounds paving the way to several applications	1951:2057	Taken together, these results highlight PEG-b-PCL NPs coupled with HPβCD as a novel vehicle for the skin delivery of highly lipophilic compounds paving the way to several applications.
26004575	10	71	theme	novel	1953:1957	arg1	NPs					1925:1927	PEG-b-PCL NPs	1915:1927	PEG-b-PCL NPs coupled with HPβCD	1915:1946	Taken together, these results highlight PEG-b-PCL NPs coupled with HPβCD as a novel vehicle for the skin delivery of highly lipophilic compounds paving the way to several applications.
26004575	8	72	theme	NPs	1487:1489	arg1	ability					1476:1482	the ability	1472:1482	the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces	1472:1586	Fluorescence images of skin samples after 24h of permeation were in line with ZnPc dosage in the skin and demonstrated the ability of NPs covalently tagged with rhodamine to penetrate the skin and to locate in the intercellular spaces.
26004575	2	73	theme	fluorescent	412:422	arg1	molecule					430:437	lipophilic and fluorescent model molecule	397:437	lipophilic and fluorescent model molecule	397:437	Zinc(II) phthalocyanine (ZnPc), selected as lipophilic and fluorescent model molecule, was loaded inside NPs by a melting/sonication procedure.
26004575	5	74	theme	2-hydroxypropyl	983:997	arg1	-β-cyclodextrin					999:1013	(2-hydroxypropyl)-β-cyclodextrin	982:1013	(2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer	982:1044	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	5	74	theme	2-hydroxypropyl	983:997	arg1	HPβCD					1016:1020	HPβCD	1016:1020	HPβCD	1016:1020	The transport of ZnPc through porcine ear skin was evaluated on Franz-type diffusion cells after treatment with different vehicles (water or PEG 0.4kDa) containing free ZnPc or ZnPc-loaded NPs without and with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) as permeation enhancer.
26004575	3	75	theme	hydrodynamic	515:526	arg1	diameter					528:535	a hydrodynamic diameter	513:535	a hydrodynamic diameter around 60nm, a slightly negative zeta potential and a ZnPc entrapment dependent on polymer/ZnPc ratio	513:637	Loaded NPs with a hydrodynamic diameter around 60nm, a slightly negative zeta potential and a ZnPc entrapment dependent on polymer/ZnPc ratio were obtained.
26004575	7	76	theme	large	1204:1208	arg1	amount					1210:1215	large amount	1204:1215	large amount	1204:1215	On the other hand, ZnPc was found in the skin in large amount and also in the viable epidermis when delivered through NPs associated with HPβCD, especially in conditions limiting water evaporation.
26004575	1	77	theme	work	125:128	arg1	aim					113:115	The aim	109:115	The aim of this work	109:128	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	9	78	theme	NPs	1647:1649	arg1	application					1632:1642	application	1632:1642	application of NPs by confocal Raman spectroscopy	1632:1680	Insight into skin chemical properties upon application of NPs by confocal Raman spectroscopy demonstrated that HPβCD caused an alteration of water profile in the skin, highly reducing the degree of hydration at stratum corneum/viable epidermis interface which can promote NP transport.
26004575	3	79	theme	dependent	607:615	arg1	entrapment					596:605	a ZnPc entrapment	589:605	a ZnPc entrapment dependent on polymer/ZnPc ratio	589:637	Loaded NPs with a hydrodynamic diameter around 60nm, a slightly negative zeta potential and a ZnPc entrapment dependent on polymer/ZnPc ratio were obtained.
26004575	1	80	theme	poly	202:205	arg1	ε-caprolactone					229:242	ε-caprolactone	229:242	ε-caprolactone	229:242	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	1	80	theme	poly	202:205	arg1	-poly					223:227	a poly(ethylene glycol)-poly	200:227	a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer	200:273	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
26004575	1	80	theme	poly	202:205	arg1	glycol					216:221	ethylene glycol	207:221	ethylene glycol	207:221	The aim of this work was to investigate the potential of small nanoparticles (NPs) made of a poly(ethylene glycol)-poly(ε-caprolactone)-amphiphilic diblock copolymer (PEG-b-PCL, PEG=2kDa and PCL=4.2kDa) as drug carrier system through the skin.
27657813	7	0	theme	nanofiber	1180:1188	arg1	membranes					1190:1198	the nanofiber membranes	1176:1198	the nanofiber membranes	1176:1198	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	7	0	theme	nanofiber	1180:1188	arg1	beneficial					1204:1213	beneficial	1204:1213	beneficial	1204:1213	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	6	1	theme	replacement	920:930	arg1	after-treatment					952:966	an after-treatment	949:966	an after-treatment for SA/PLA nanofiber membranes	949:997	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	6	1	theme	replacement	920:930	arg1	step					932:935	A calcium ion replacement step	906:935	A calcium ion replacement step	906:935	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	7	2	theme	tensile	1095:1101	arg1	test					1103:1106	The single fiber tensile test	1078:1106	The single fiber tensile test	1078:1106	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	3	3	theme	property	542:549	arg1	biocompatibility					571:586	biocompatibility	571:586	biocompatibility	571:586	Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility.
27657813	3	3	theme	property	542:549	arg1	hydrophilicity					552:565	hydrophilicity	552:565	hydrophilicity	552:565	Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility.
27657813	3	3	theme	property	542:549	arg1	areas					506:510	biomedical areas	495:510	biomedical areas because of its anti-bacterial property	495:549	Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility.
27657813	6	4	theme	ion	916:918	arg1	after-treatment					952:966	an after-treatment	949:966	an after-treatment for SA/PLA nanofiber membranes	949:997	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	6	4	theme	ion	916:918	arg1	step					932:935	A calcium ion replacement step	906:935	A calcium ion replacement step	906:935	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	6	5	from	anchor	1011:1016	arg1	form					1039:1042	a form	1037:1042	a form of gelated calcium alginate (CA)	1037:1075	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	7	6	theme	cell	1219:1222	arg1	proliferation					1224:1236	cell proliferation	1219:1236	cell proliferation	1219:1236	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	2	7	with	membranes	328:336	arg1	strength					390:397	good mechanical strength	374:397	good mechanical strength	374:397	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	2	7	with	membranes	328:336	arg1	biocompatibility					353:368	favorable biocompatibility	343:368	favorable biocompatibility	343:368	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	6	8	theme	calcium	908:914	arg1	after-treatment					952:966	an after-treatment	949:966	an after-treatment for SA/PLA nanofiber membranes	949:997	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	6	8	theme	calcium	908:914	arg1	step					932:935	A calcium ion replacement step	906:935	A calcium ion replacement step	906:935	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	7	9	theme	single	1082:1087	arg1	test					1103:1106	The single fiber tensile test	1078:1106	The single fiber tensile test	1078:1106	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	5	10	located	located	820:826	arg2	existence					800:808	the existence	796:808	the existence of SA	796:814	The CLSM images illustrated that the existence of SA was located on the surface of composite fibers and the FTIR results confirmed the result.
27657813	5	10	located	located	820:826	arg2	located					820:826	located	820:826	located	820:826	The CLSM images illustrated that the existence of SA was located on the surface of composite fibers and the FTIR results confirmed the result.
27657813	5	10	located	located	820:826	arg1	surface					835:841	the surface	831:841	the surface of composite fibers	831:861	The CLSM images illustrated that the existence of SA was located on the surface of composite fibers and the FTIR results confirmed the result.
27657813	2	11	theme	nanofiber	318:326	arg1	membranes					328:336	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes	264:336	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength	264:397	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	0	12	theme	emulsion	106:113	arg1	eletrospinning					115:128	emulsion eletrospinning	106:128	emulsion eletrospinning	106:128	Preparation and characterization of electrospun alginate/PLA nanofibers as tissue engineering material by emulsion eletrospinning.
27657813	7	13	theme	fiber	1089:1093	arg1	test					1103:1106	The single fiber tensile test	1078:1106	The single fiber tensile test	1078:1106	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	4	14	theme	continuous	622:631	arg1	phase					633:637	continuous phase	622:637	continuous phase	622:637	In this article, we chose PLA as continuous phase and SA as dispersion phase to prepare a W/O emulsion and then electrospun it to get a SA/PLA composite nanofiber membranes.
27657813	7	15	theme	membranes	1161:1169	arg1	property					1132:1139	a good mechanical property	1114:1139	a good mechanical property of CA/PLA nanofiber membranes	1114:1169	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	5	16	theme	composite	846:854	arg1	fibers					856:861	composite fibers	846:861	composite fibers	846:861	The CLSM images illustrated that the existence of SA was located on the surface of composite fibers and the FTIR results confirmed the result.
27657813	6	17	theme	calcium	1055:1061	arg1	CA					1073:1074	CA	1073:1074	CA	1073:1074	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	6	17	theme	calcium	1055:1061	arg1	alginate					1063:1070	gelated calcium alginate	1047:1070	gelated calcium alginate (CA)	1047:1075	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	7	18	theme	S	1301:1301	arg1	staining					1309:1316	Alizarin red S (ARS) staining	1288:1316	MTT array as well as Alizarin red S (ARS) staining test	1267:1321	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	5	19	theme	CLSM	767:770	arg1	images					772:777	The CLSM images	763:777	The CLSM images	763:777	The CLSM images illustrated that the existence of SA was located on the surface of composite fibers and the FTIR results confirmed the result.
27657813	7	20	theme	good	1116:1119	arg1	property					1132:1139	a good mechanical property	1114:1139	a good mechanical property of CA/PLA nanofiber membranes	1114:1169	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	6	21	theme	alginic	1022:1028	arg1	ion					1030:1032	the alginic ion	1018:1032	the alginic ion	1018:1032	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	2	22	theme	engineering	435:445	arg1	scaffold					447:454	an innovative tissue engineering scaffold	414:454	an innovative tissue engineering scaffold	414:454	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	7	23	theme	CA/PLA	1144:1149	arg1	membranes					1161:1169	CA/PLA nanofiber membranes	1144:1169	CA/PLA nanofiber membranes	1144:1169	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	2	24	theme	tissue	428:433	arg1	engineering					435:445	an innovative tissue engineering	414:445	an innovative tissue engineering scaffold	414:454	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	7	25	theme	nanofiber	1151:1159	arg1	membranes					1161:1169	CA/PLA nanofiber membranes	1144:1169	CA/PLA nanofiber membranes	1144:1169	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	6	26	theme	nanofiber	979:987	arg1	membranes					989:997	SA/PLA nanofiber membranes	972:997	SA/PLA nanofiber membranes	972:997	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	2	27	theme	favorable	343:351	arg1	biocompatibility					353:368	favorable biocompatibility	343:368	favorable biocompatibility	343:368	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	2	28	theme	innovative	417:426	arg1	engineering					435:445	an innovative tissue engineering	414:445	an innovative tissue engineering scaffold	414:454	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	6	29	theme	SA/PLA	972:977	arg1	membranes					989:997	SA/PLA nanofiber membranes	972:997	SA/PLA nanofiber membranes	972:997	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	4	30	dep	SA/PLA	725:730	arg1	membranes					752:760	composite nanofiber membranes	732:760	a SA/PLA composite nanofiber membranes	723:760	In this article, we chose PLA as continuous phase and SA as dispersion phase to prepare a W/O emulsion and then electrospun it to get a SA/PLA composite nanofiber membranes.
27657813	6	31	theme	alginate	1063:1070	arg1	form					1039:1042	a form	1037:1042	a form of gelated calcium alginate (CA)	1037:1075	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	1	32	theme	wide	214:217	arg1	application					229:239	a wide potential application	212:239	a wide potential application in tissue engineering	212:261	Scaffolds made by biomaterials offer favorite environment for cell grow and show a wide potential application in tissue engineering.
27657813	3	33	used	used	487:490	arg2	SA					474:475	SA	474:475	SA	474:475	Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility.
27657813	3	33	used	used	487:490	arg2	alginate					464:471	Sodium alginate	457:471	Sodium alginate (SA)	457:476	Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility.
27657813	6	34	used	used	941:944	arg2	step					932:935	A calcium ion replacement step	906:935	A calcium ion replacement step	906:935	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	6	34	used	used	941:944	arg2	after-treatment					952:966	an after-treatment	949:966	an after-treatment for SA/PLA nanofiber membranes	949:997	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	1	35	dep	environment	177:187	arg1	show					207:210	show	207:210	show a wide potential application in tissue engineering	207:261	Scaffolds made by biomaterials offer favorite environment for cell grow and show a wide potential application in tissue engineering.
27657813	1	35	dep	environment	177:187	arg1	grow					198:201	grow	198:201	grow	198:201	Scaffolds made by biomaterials offer favorite environment for cell grow and show a wide potential application in tissue engineering.
27657813	1	36	theme	potential	219:227	arg1	application					229:239	a wide potential application	212:239	a wide potential application in tissue engineering	212:261	Scaffolds made by biomaterials offer favorite environment for cell grow and show a wide potential application in tissue engineering.
27657813	0	37	theme	alginate/PLA	48:59	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of electrospun alginate/PLA nanofibers as tissue engineering material by emulsion eletrospinning.
27657813	0	37	theme	alginate/PLA	48:59	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of electrospun alginate/PLA nanofibers as tissue engineering material by emulsion eletrospinning.
27657813	3	38	theme	anti-bacterial	527:540	arg1	property					542:549	its anti-bacterial property	523:549	its anti-bacterial property	523:549	Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility.
27657813	2	39	theme	Novel	264:268	arg1	materials					287:295	Novel biocompatibility materials	264:295	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength	264:397	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	6	40	theme	gelated	1047:1053	arg1	CA					1073:1074	CA	1073:1074	CA	1073:1074	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	6	40	theme	gelated	1047:1053	arg1	alginate					1063:1070	gelated calcium alginate	1047:1070	gelated calcium alginate (CA)	1047:1075	A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA).
27657813	5	41	theme	fibers	856:861	arg1	surface					835:841	the surface	831:841	the surface of composite fibers	831:861	The CLSM images illustrated that the existence of SA was located on the surface of composite fibers and the FTIR results confirmed the result.
27657813	0	42	theme	electrospun	36:46	arg1	alginate/PLA					48:59	electrospun alginate/PLA	36:59	electrospun alginate/PLA	36:59	Preparation and characterization of electrospun alginate/PLA nanofibers as tissue engineering material by emulsion eletrospinning.
27657813	5	43	theme	FTIR	871:874	arg1	results					876:882	the FTIR results	867:882	the FTIR results	867:882	The CLSM images illustrated that the existence of SA was located on the surface of composite fibers and the FTIR results confirmed the result.
27657813	7	44	theme	mechanical	1121:1130	arg1	property					1132:1139	a good mechanical property	1114:1139	a good mechanical property of CA/PLA nanofiber membranes	1114:1169	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	1	45	from	application	229:239	arg1	engineering					251:261	tissue engineering	244:261	tissue engineering	244:261	Scaffolds made by biomaterials offer favorite environment for cell grow and show a wide potential application in tissue engineering.
27657813	5	46	theme	SA	813:814	arg1	existence					800:808	the existence	796:808	the existence of SA	796:814	The CLSM images illustrated that the existence of SA was located on the surface of composite fibers and the FTIR results confirmed the result.
27657813	5	46	theme	SA	813:814	arg1	located					820:826	located	820:826	located	820:826	The CLSM images illustrated that the existence of SA was located on the surface of composite fibers and the FTIR results confirmed the result.
27657813	7	47	theme	ARS	1304:1306	arg1	staining					1309:1316	Alizarin red S (ARS) staining	1288:1316	MTT array as well as Alizarin red S (ARS) staining test	1267:1321	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	3	48	theme	Sodium	457:462	arg1	SA					474:475	SA	474:475	SA	474:475	Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility.
27657813	3	48	theme	Sodium	457:462	arg1	alginate					464:471	Sodium alginate	457:471	Sodium alginate (SA)	457:476	Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility.
27657813	7	49	theme	red	1297:1299	arg1	staining					1309:1316	Alizarin red S (ARS) staining	1288:1316	MTT array as well as Alizarin red S (ARS) staining test	1267:1321	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	2	50	theme	mechanical	379:388	arg1	strength					390:397	good mechanical strength	374:397	good mechanical strength	374:397	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	7	51	theme	staining	1309:1316	arg1	test					1318:1321	Alizarin red S (ARS) staining test	1288:1321	MTT array as well as Alizarin red S (ARS) staining test	1267:1321	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	2	52	theme	acid	307:310	arg1	membranes					328:336	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes	264:336	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength	264:397	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	2	53	theme	good	374:377	arg1	strength					390:397	good mechanical strength	374:397	good mechanical strength	374:397	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	1	54	theme	tissue	244:249	arg1	engineering					251:261	tissue engineering	244:261	tissue engineering	244:261	Scaffolds made by biomaterials offer favorite environment for cell grow and show a wide potential application in tissue engineering.
27657813	7	55	theme	MTT	1267:1269	arg1	array					1271:1275	MTT array	1267:1275	MTT array as well as Alizarin red S (ARS) staining test	1267:1321	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	2	56	theme	polylatic	297:305	arg1	PLA					313:315	PLA	313:315	PLA	313:315	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	2	56	theme	polylatic	297:305	arg1	acid					307:310	polylatic acid	297:310	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength	264:397	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	4	57	theme	composite	732:740	arg1	membranes					752:760	composite nanofiber membranes	732:760	a SA/PLA composite nanofiber membranes	723:760	In this article, we chose PLA as continuous phase and SA as dispersion phase to prepare a W/O emulsion and then electrospun it to get a SA/PLA composite nanofiber membranes.
27657813	7	58	theme	Alizarin	1288:1295	arg1	staining					1309:1316	Alizarin red S (ARS) staining	1288:1316	MTT array as well as Alizarin red S (ARS) staining test	1267:1321	The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
27657813	4	59	theme	nanofiber	742:750	arg1	membranes					752:760	composite nanofiber membranes	732:760	a SA/PLA composite nanofiber membranes	723:760	In this article, we chose PLA as continuous phase and SA as dispersion phase to prepare a W/O emulsion and then electrospun it to get a SA/PLA composite nanofiber membranes.
27657813	0	60	theme	engineering	82:92	arg1	material					94:101	tissue engineering material	75:101	tissue engineering material by emulsion eletrospinning	75:128	Preparation and characterization of electrospun alginate/PLA nanofibers as tissue engineering material by emulsion eletrospinning.
27657813	2	61	theme	materials	287:295	arg1	membranes					328:336	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes	264:336	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength	264:397	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	3	62	theme	biomedical	495:504	arg1	biocompatibility					571:586	biocompatibility	571:586	biocompatibility	571:586	Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility.
27657813	3	62	theme	biomedical	495:504	arg1	hydrophilicity					552:565	hydrophilicity	552:565	hydrophilicity	552:565	Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility.
27657813	3	62	theme	biomedical	495:504	arg1	areas					506:510	biomedical areas	495:510	biomedical areas because of its anti-bacterial property	495:549	Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility.
27657813	1	63	theme	favorite	168:175	arg1	environment					177:187	favorite environment	168:187	favorite environment for cell grow and show a wide potential application in tissue engineering	168:261	Scaffolds made by biomaterials offer favorite environment for cell grow and show a wide potential application in tissue engineering.
27657813	4	64	theme	W/O	679:681	arg1	emulsion					683:690	a W/O emulsion	677:690	a W/O emulsion	677:690	In this article, we chose PLA as continuous phase and SA as dispersion phase to prepare a W/O emulsion and then electrospun it to get a SA/PLA composite nanofiber membranes.
27657813	4	65	theme	dispersion	649:658	arg1	phase					660:664	SA as dispersion phase	643:664	SA as dispersion phase	643:664	In this article, we chose PLA as continuous phase and SA as dispersion phase to prepare a W/O emulsion and then electrospun it to get a SA/PLA composite nanofiber membranes.
27657813	0	66	theme	tissue	75:80	arg1	material					94:101	tissue engineering material	75:101	tissue engineering material by emulsion eletrospinning	75:128	Preparation and characterization of electrospun alginate/PLA nanofibers as tissue engineering material by emulsion eletrospinning.
27657813	2	67	theme	biocompatibility	270:285	arg1	materials					287:295	Novel biocompatibility materials	264:295	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength	264:397	Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold.
27657813	4	68	theme	SA	643:644	arg1	phase					660:664	SA as dispersion phase	643:664	SA as dispersion phase	643:664	In this article, we chose PLA as continuous phase and SA as dispersion phase to prepare a W/O emulsion and then electrospun it to get a SA/PLA composite nanofiber membranes.
27657813	4	69	theme	as	646:647	arg1	phase					660:664	SA as dispersion phase	643:664	SA as dispersion phase	643:664	In this article, we chose PLA as continuous phase and SA as dispersion phase to prepare a W/O emulsion and then electrospun it to get a SA/PLA composite nanofiber membranes.
25277411	0	0	theme	steroid-transforming	102:121	arg1	simplex					136:142	steroid-transforming Arthrobacter simplex	102:142	steroid-transforming Arthrobacter simplex	102:142	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	6	1	theme	total	1014:1018	arg1	leakage					1020:1026	The total leakage	1010:1026	The total leakage of protein level	1010:1043	The total leakage of protein level increased to 8%.
25277411	2	2	theme	comparative	432:442	arg1	investigation					444:456	a comparative investigation	430:456	a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD	430:577	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	0	3	from	alterations	38:48	arg1	permeability					58:69	cell permeability	53:69	cell permeability	53:69	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	8	4	from	change	1221:1226	arg1	level					1261:1265	the molecular level	1247:1265	the molecular level under HP-β-CD treatment	1247:1289	These alterations can explain the change of permeability on the molecular level under HP-β-CD treatment.
25277411	0	5	theme	simplex	136:142	arg1	profiles					90:97	lipid and protein profiles	72:97	profiles	90:97	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	0	5	theme	simplex	136:142	arg1	alterations					38:48	Hydroxypropyl-β-cyclodextrin-mediated alterations	0:48	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability	0:69	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	9	6	theme	conversion	1411:1420	arg1	rate					1422:1425	the conversion rate	1407:1425	the conversion rate in addition to cyclodextrins	1407:1454	Results showed the material basis and mechanisms underlying the cellular changes, thus most likely contributing to the conversion rate in addition to cyclodextrins known effects on substrate solubility.
25277411	3	7	theme	transmission	608:619	arg1	microscopy					630:639	transmission electron microscopy	608:639	transmission electron microscopy	608:639	Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope.
25277411	2	8	theme	positive	367:374	arg1	effects					376:382	positive effects	367:382	positive effects	367:382	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	0	9	theme	Arthrobacter	123:134	arg1	simplex					136:142	steroid-transforming Arthrobacter simplex	102:142	steroid-transforming Arthrobacter simplex	102:142	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	3	10	theme	microscopy	593:602	arg1	micrographs					641:651	Atomic force microscopy and transmission electron microscopy micrographs	580:651	Atomic force microscopy and transmission electron microscopy micrographs	580:651	Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope.
25277411	4	11	theme	lipids	824:829	arg1	proportion					798:807	the proportion	794:807	the proportion of extractable lipids	794:829	The analysis of lipid composition revealed that the proportion of extractable lipids decreased and the fatty acids profile was considerably altered.
25277411	4	12	theme	extractable	812:822	arg1	lipids					824:829	extractable lipids	812:829	extractable lipids	812:829	The analysis of lipid composition revealed that the proportion of extractable lipids decreased and the fatty acids profile was considerably altered.
25277411	5	13	theme	fatty	950:954	arg1	acids					956:960	long-chain fatty acids	939:960	long-chain fatty acids	939:960	The contents of unsaturated fatty acids and long-chain fatty acids were reduced by 11.77 and 14.98%, respectively.
25277411	5	14	theme	unsaturated	911:921	arg1	acids					929:933	unsaturated fatty acids	911:933	unsaturated fatty acids	911:933	The contents of unsaturated fatty acids and long-chain fatty acids were reduced by 11.77 and 14.98%, respectively.
25277411	3	15	theme	microscopy	630:639	arg1	micrographs					641:651	Atomic force microscopy and transmission electron microscopy micrographs	580:651	Atomic force microscopy and transmission electron microscopy micrographs	580:651	Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope.
25277411	1	16	theme	Arthrobacter	240:251	arg1	simplex					253:259	Arthrobacter simplex	240:259	Arthrobacter simplex	240:259	Hydroxypropyl-β-cyclodextrin (HP-β-CD) enhances steroid 1-dehydrogenation biotransformation by Arthrobacter simplex.
25277411	8	17	theme	HP-β-CD	1273:1279	arg1	treatment					1281:1289	HP-β-CD treatment	1273:1289	HP-β-CD treatment	1273:1289	These alterations can explain the change of permeability on the molecular level under HP-β-CD treatment.
25277411	9	18	theme	material	1311:1318	arg1	basis					1320:1324	the material basis	1307:1324	the material basis	1307:1324	Results showed the material basis and mechanisms underlying the cellular changes, thus most likely contributing to the conversion rate in addition to cyclodextrins known effects on substrate solubility.
25277411	2	19	theme	permeability	344:355	arg1	improvement					292:302	HP-β-CD-induced improvement	276:302	HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion	276:411	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	7	20	dep	superfamily	1109:1119	arg1	the					1084:1086	the	1084:1086	the	1084:1086	Proteins belonging to the ATP-binding cassette superfamily and major facilitator superfamily were observed outside the cell.
25277411	2	21	theme	lower	473:477	arg1	activity					493:500	a lower dehydrogenase activity	471:500	a lower dehydrogenase activity	471:500	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	2	22	theme	cells	538:542	arg1	permeability					518:529	higher cell permeability	506:529	higher cell permeability	506:529	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	2	22	theme	cells	538:542	arg1	activity					493:500	a lower dehydrogenase activity	471:500	a lower dehydrogenase activity	471:500	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	2	23	theme	steroid	391:397	arg1	bioconversion					399:411	the steroid bioconversion	387:411	the steroid bioconversion	387:411	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	9	24	from	effects	1462:1468	arg1	solubility					1483:1492	substrate solubility	1473:1492	substrate solubility	1473:1492	Results showed the material basis and mechanisms underlying the cellular changes, thus most likely contributing to the conversion rate in addition to cyclodextrins known effects on substrate solubility.
25277411	4	25	theme	composition	768:778	arg1	analysis					750:757	The analysis	746:757	The analysis of lipid composition	746:778	The analysis of lipid composition revealed that the proportion of extractable lipids decreased and the fatty acids profile was considerably altered.
25277411	0	26	theme	Hydroxypropyl-β-cyclodextrin-mediated	0:36	arg1	alterations					38:48	Hydroxypropyl-β-cyclodextrin-mediated alterations	0:48	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability	0:69	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	0	26	theme	Hydroxypropyl-β-cyclodextrin-mediated	0:36	arg1	profiles					90:97	lipid and protein profiles	72:97	profiles	90:97	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	5	27	theme	fatty	923:927	arg1	acids					929:933	unsaturated fatty acids	911:933	unsaturated fatty acids	911:933	The contents of unsaturated fatty acids and long-chain fatty acids were reduced by 11.77 and 14.98%, respectively.
25277411	8	28	theme	molecular	1251:1259	arg1	level					1261:1265	the molecular level	1247:1265	the molecular level under HP-β-CD treatment	1247:1289	These alterations can explain the change of permeability on the molecular level under HP-β-CD treatment.
25277411	4	29	theme	lipid	762:766	arg1	composition					768:778	lipid composition	762:778	lipid composition	762:778	The analysis of lipid composition revealed that the proportion of extractable lipids decreased and the fatty acids profile was considerably altered.
25277411	0	30	theme	cell	53:56	arg1	permeability					58:69	cell permeability	53:69	cell permeability	53:69	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	3	31	theme	surface	706:712	arg1	structure					714:722	surface structure	706:722	surface structure	706:722	Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope.
25277411	4	32	theme	acids	855:859	arg1	profile					861:867	the fatty acids profile	845:867	the fatty acids profile	845:867	The analysis of lipid composition revealed that the proportion of extractable lipids decreased and the fatty acids profile was considerably altered.
25277411	2	33	theme	cell	513:516	arg1	permeability					518:529	higher cell permeability	506:529	higher cell permeability	506:529	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	2	34	contain	had	363:365	arg1	permeability					344:355	A. simplex CPCC 140451 cell envelope permeability	307:355	A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion	307:411	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	2	34	contain	had	363:365	arg2	effects					376:382	positive effects	367:382	positive effects	367:382	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	4	35	theme	fatty	849:853	arg1	profile					861:867	the fatty acids profile	845:867	the fatty acids profile	845:867	The analysis of lipid composition revealed that the proportion of extractable lipids decreased and the fatty acids profile was considerably altered.
25277411	2	36	theme	higher	506:511	arg1	permeability					518:529	higher cell permeability	506:529	higher cell permeability	506:529	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	2	37	theme	simplex	310:316	arg1	permeability					344:355	A. simplex CPCC 140451 cell envelope permeability	307:355	A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion	307:411	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	5	38	theme	acids	956:960	arg1	contents					899:906	The contents	895:906	The contents of unsaturated fatty acids and long-chain fatty acids	895:960	The contents of unsaturated fatty acids and long-chain fatty acids were reduced by 11.77 and 14.98%, respectively.
25277411	2	39	theme	dehydrogenase	479:491	arg1	activity					493:500	a lower dehydrogenase activity	471:500	a lower dehydrogenase activity	471:500	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	9	40	theme	substrate	1473:1481	arg1	solubility					1483:1492	substrate solubility	1473:1492	substrate solubility	1473:1492	Results showed the material basis and mechanisms underlying the cellular changes, thus most likely contributing to the conversion rate in addition to cyclodextrins known effects on substrate solubility.
25277411	9	41	theme	cellular	1356:1363	arg1	changes					1365:1371	the cellular changes	1352:1371	the cellular changes	1352:1371	Results showed the material basis and mechanisms underlying the cellular changes, thus most likely contributing to the conversion rate in addition to cyclodextrins known effects on substrate solubility.
25277411	3	42	theme	cell	731:734	arg1	envelope					736:743	the cell envelope	727:743	the cell envelope	727:743	Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope.
25277411	2	43	theme	A.	307:308	arg1	permeability					344:355	A. simplex CPCC 140451 cell envelope permeability	307:355	A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion	307:411	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	5	44	theme	acids	929:933	arg1	contents					899:906	The contents	895:906	The contents of unsaturated fatty acids and long-chain fatty acids	895:960	The contents of unsaturated fatty acids and long-chain fatty acids were reduced by 11.77 and 14.98%, respectively.
25277411	6	45	theme	level	1039:1043	arg1	leakage					1020:1026	The total leakage	1010:1026	The total leakage of protein level	1010:1043	The total leakage of protein level increased to 8%.
25277411	3	46	theme	envelope	736:743	arg1	structure					714:722	surface structure	706:722	surface structure	706:722	Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope.
25277411	3	46	theme	envelope	736:743	arg1	sharpness					691:699	sharpness	691:699	sharpness	691:699	Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope.
25277411	3	46	theme	envelope	736:743	arg1	size					685:688	size	685:688	size	685:688	Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope.
25277411	3	47	theme	Atomic	580:585	arg1	microscopy					593:602	Atomic force microscopy	580:602	Atomic force microscopy	580:602	Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope.
25277411	0	48	theme	lipid	72:76	arg1	profiles					90:97	lipid and protein profiles	72:97	profiles	90:97	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	0	48	theme	lipid	72:76	arg1	alterations					38:48	Hydroxypropyl-β-cyclodextrin-mediated alterations	0:48	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability	0:69	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	6	49	theme	protein	1031:1037	arg1	level					1039:1043	protein level	1031:1043	protein level	1031:1043	The total leakage of protein level increased to 8%.
25277411	2	50	theme	HP-β-CD-induced	276:290	arg1	improvement					292:302	HP-β-CD-induced improvement	276:302	HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion	276:411	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	7	51	theme	ATP-binding	1088:1098	arg1	superfamily					1109:1119	ATP-binding cassette superfamily	1088:1119	ATP-binding cassette superfamily	1088:1119	Proteins belonging to the ATP-binding cassette superfamily and major facilitator superfamily were observed outside the cell.
25277411	3	52	theme	force	587:591	arg1	microscopy					593:602	Atomic force microscopy	580:602	Atomic force microscopy	580:602	Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope.
25277411	9	53	dep	cyclodextrins	1442:1454	arg1	addition					1430:1437	addition	1430:1437	addition	1430:1437	Results showed the material basis and mechanisms underlying the cellular changes, thus most likely contributing to the conversion rate in addition to cyclodextrins known effects on substrate solubility.
25277411	7	54	theme	major	1125:1129	arg1	superfamily					1143:1153	major facilitator superfamily	1125:1153	major facilitator superfamily	1125:1153	Proteins belonging to the ATP-binding cassette superfamily and major facilitator superfamily were observed outside the cell.
25277411	2	55	theme	envelope	335:342	arg1	permeability					344:355	A. simplex CPCC 140451 cell envelope permeability	307:355	A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion	307:411	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	7	56	theme	cassette	1100:1107	arg1	superfamily					1109:1119	ATP-binding cassette superfamily	1088:1119	ATP-binding cassette superfamily	1088:1119	Proteins belonging to the ATP-binding cassette superfamily and major facilitator superfamily were observed outside the cell.
25277411	7	57	theme	facilitator	1131:1141	arg1	superfamily					1143:1153	major facilitator superfamily	1125:1153	major facilitator superfamily	1125:1153	Proteins belonging to the ATP-binding cassette superfamily and major facilitator superfamily were observed outside the cell.
25277411	2	58	theme	cell	330:333	arg1	permeability					344:355	A. simplex CPCC 140451 cell envelope permeability	307:355	A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion	307:411	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	9	59	theme	known	1456:1460	arg1	effects					1462:1468	known effects	1456:1468	known effects on substrate solubility	1456:1492	Results showed the material basis and mechanisms underlying the cellular changes, thus most likely contributing to the conversion rate in addition to cyclodextrins known effects on substrate solubility.
25277411	3	60	theme	electron	621:628	arg1	microscopy					630:639	transmission electron microscopy	608:639	transmission electron microscopy	608:639	Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope.
25277411	8	61	theme	permeability	1231:1242	arg1	change					1221:1226	the change	1217:1226	the change of permeability on the molecular level under HP-β-CD treatment	1217:1289	These alterations can explain the change of permeability on the molecular level under HP-β-CD treatment.
25277411	2	62	theme	140451	323:328	arg1	permeability					344:355	A. simplex CPCC 140451 cell envelope permeability	307:355	A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion	307:411	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	5	63	theme	long-chain	939:948	arg1	acids					956:960	long-chain fatty acids	939:960	long-chain fatty acids	939:960	The contents of unsaturated fatty acids and long-chain fatty acids were reduced by 11.77 and 14.98%, respectively.
25277411	1	64	theme	steroid	193:199	arg1	biotransformation					219:235	steroid 1-dehydrogenation biotransformation	193:235	steroid 1-dehydrogenation biotransformation	193:235	Hydroxypropyl-β-cyclodextrin (HP-β-CD) enhances steroid 1-dehydrogenation biotransformation by Arthrobacter simplex.
25277411	0	65	theme	protein	82:88	arg1	profiles					90:97	lipid and protein profiles	72:97	profiles	90:97	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	0	65	theme	protein	82:88	arg1	alterations					38:48	Hydroxypropyl-β-cyclodextrin-mediated alterations	0:48	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability	0:69	Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex.
25277411	2	66	theme	CPCC	318:321	arg1	permeability					344:355	A. simplex CPCC 140451 cell envelope permeability	307:355	A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion	307:411	In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.
25277411	1	67	theme	1-dehydrogenation	201:217	arg1	biotransformation					219:235	steroid 1-dehydrogenation biotransformation	193:235	steroid 1-dehydrogenation biotransformation	193:235	Hydroxypropyl-β-cyclodextrin (HP-β-CD) enhances steroid 1-dehydrogenation biotransformation by Arthrobacter simplex.
24458309	1	0	from	absence	270:276	arg1	effect					208:213	the effect	204:213	the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality	204:345	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	4	1	theme	volume	783:788	arg1	solutions					802:810	volume replacement solutions	783:810	volume replacement solutions	783:810	RESULTS There was not any influence of volume replacement solutions on the release of the enzymes from resting neutrophils.
24458309	1	2	theme	hydroxyethyl	293:304	arg1	HES					314:316	HES	314:316	HES	314:316	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	1	2	theme	hydroxyethyl	293:304	arg1	starch					306:311	hydroxyethyl starch	293:311	hydroxyethyl starch (HES)	293:317	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	1	3	theme	metalloproteinase	381:397	arg1	release					363:369	the release	359:369	the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8	359:439	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	8	4	theme	Volume	1275:1280	arg1	solutions					1294:1302	Volume replacement solutions	1275:1302	CONCLUSION Volume replacement solutions	1264:1302	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	1	5	dep	balanced	218:225	arg1	BS					228:229	BS	228:229	BS	228:229	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	1	6	theme	starch	306:311	arg1	presence					281:288	presence	281:288	presence	281:288	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	1	6	theme	starch	306:311	arg1	absence					270:276	the absence	266:276	the absence	266:276	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	4	7	theme	replacement	790:800	arg1	solutions					802:810	volume replacement solutions	783:810	volume replacement solutions	783:810	RESULTS There was not any influence of volume replacement solutions on the release of the enzymes from resting neutrophils.
24458309	8	8	theme	unbalanced	1426:1435	arg1	solutions					1437:1445	balanced or unbalanced solutions	1414:1445	balanced or unbalanced solutions	1414:1445	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	8	9	theme	MMP-9	1343:1347	arg1	release					1332:1338	the release	1328:1338	the release of MMP-9	1328:1347	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	2	10	theme	dextran	521:527	arg1	sedimentation					529:541	dextran sedimentation	521:541	dextran sedimentation	521:541	MATERIALS AND METHODS Neutrophils were isolated by gradient centrifugation and dextran sedimentation and incubated in BS or UBS without or with HES, in the absence or presence of Interleukin-8 (IL-8) or Lipopolysaccharide (LPS).
24458309	1	11	theme	unbalanced	236:245	arg1	solutions					253:261	balanced (BS) and unbalanced (UBS) solutions	218:261	balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES)	218:317	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	3	12	theme	ELISA	732:736	arg1	kits					738:741	commercially available ELISA kits	709:741	commercially available ELISA kits	709:741	MMP-9, MPO, and MMP-8 were assayed by commercially available ELISA kits.
24458309	4	13	theme	enzymes	834:840	arg1	release					819:825	the release	815:825	the release of the enzymes from resting neutrophils	815:865	RESULTS There was not any influence of volume replacement solutions on the release of the enzymes from resting neutrophils.
24458309	1	14	from	solutions	253:261	arg1	presence					281:288	presence	281:288	presence	281:288	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	1	14	from	solutions	253:261	arg1	absence					270:276	the absence	266:276	the absence	266:276	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	6	15	theme	LPS	1026:1028	arg1	stimulation					1030:1040	LPS stimulation	1026:1040	LPS stimulation	1026:1040	After LPS stimulation, the release of MMP-9 was higher in both UBS and BS than RPMI-1640, but HES brought its release back to physiological conditions.
24458309	4	16	dep	RESULTS	744:750	arg1	was					758:760	was	758:760	was not any influence of volume replacement solutions on the release of the enzymes from resting neutrophils	758:865	RESULTS There was not any influence of volume replacement solutions on the release of the enzymes from resting neutrophils.
24458309	3	17	theme	available	722:730	arg1	kits					738:741	commercially available ELISA kits	709:741	commercially available ELISA kits	709:741	MMP-9, MPO, and MMP-8 were assayed by commercially available ELISA kits.
24458309	2	18	theme	Lipopolysaccharide	645:662	arg1	presence					609:616	presence	609:616	presence	609:616	MATERIALS AND METHODS Neutrophils were isolated by gradient centrifugation and dextran sedimentation and incubated in BS or UBS without or with HES, in the absence or presence of Interleukin-8 (IL-8) or Lipopolysaccharide (LPS).
24458309	2	18	theme	Lipopolysaccharide	645:662	arg1	absence					598:604	absence	598:604	absence	598:604	MATERIALS AND METHODS Neutrophils were isolated by gradient centrifugation and dextran sedimentation and incubated in BS or UBS without or with HES, in the absence or presence of Interleukin-8 (IL-8) or Lipopolysaccharide (LPS).
24458309	0	19	theme	Balanced	0:7	arg1	solutions					24:32	Balanced and unbalanced solutions	0:32	Balanced and unbalanced solutions	0:32	Balanced and unbalanced solutions modulate the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils in response to inflammatory stimuli: an in vitro study.
24458309	8	20	theme	inflammatory	1366:1377	arg1	milieu					1379:1384	the inflammatory milieu	1362:1384	the inflammatory milieu	1362:1384	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	4	21	from	influence	770:778	arg1	release					819:825	the release	815:825	the release of the enzymes from resting neutrophils	815:865	RESULTS There was not any influence of volume replacement solutions on the release of the enzymes from resting neutrophils.
24458309	1	22	theme	myeloperoxidase	408:422	arg1	release					363:369	the release	359:369	the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8	359:439	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	0	23	theme	unbalanced	13:22	arg1	solutions					24:32	Balanced and unbalanced solutions	0:32	Balanced and unbalanced solutions	0:32	Balanced and unbalanced solutions modulate the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils in response to inflammatory stimuli: an in vitro study.
24458309	4	24	theme	solutions	802:810	arg1	influence					770:778	any influence	766:778	any influence of volume replacement solutions on the release of the enzymes from resting neutrophils	766:865	RESULTS There was not any influence of volume replacement solutions on the release of the enzymes from resting neutrophils.
24458309	1	25	theme	neutrophil	322:331	arg1	functionality					333:345	neutrophil functionality	322:345	neutrophil functionality	322:345	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	7	26	theme	MMP-8	1222:1226	arg1	release					1203:1209	the release	1199:1209	the release of MPO and MMP-8 after stimulation with IL-8 or LPS	1199:1261	No difference was found in the release of MPO and MMP-8 after stimulation with IL-8 or LPS.
24458309	1	27	theme	solutions	253:261	arg1	effect					208:213	the effect	204:213	the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality	204:345	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	1	28	dep	OBJECTIVES	166:175	arg1	investigated					191:202	investigated	191:202	investigated	191:202	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	0	29	theme	inflammatory	125:136	arg1	stimuli					138:144	inflammatory stimuli	125:144	inflammatory stimuli: an in vitro study	125:163	Balanced and unbalanced solutions modulate the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils in response to inflammatory stimuli: an in vitro study.
24458309	8	30	theme	replacement	1282:1292	arg1	solutions					1294:1302	Volume replacement solutions	1275:1302	CONCLUSION Volume replacement solutions	1264:1302	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	4	31	from	neutrophils	855:865	arg1	release					819:825	the release	815:825	the release of the enzymes from resting neutrophils	815:865	RESULTS There was not any influence of volume replacement solutions on the release of the enzymes from resting neutrophils.
24458309	2	32	theme	Interleukin-8	621:633	arg1	presence					609:616	presence	609:616	presence	609:616	MATERIALS AND METHODS Neutrophils were isolated by gradient centrifugation and dextran sedimentation and incubated in BS or UBS without or with HES, in the absence or presence of Interleukin-8 (IL-8) or Lipopolysaccharide (LPS).
24458309	2	32	theme	Interleukin-8	621:633	arg1	absence					598:604	absence	598:604	absence	598:604	MATERIALS AND METHODS Neutrophils were isolated by gradient centrifugation and dextran sedimentation and incubated in BS or UBS without or with HES, in the absence or presence of Interleukin-8 (IL-8) or Lipopolysaccharide (LPS).
24458309	1	33	from	presence	281:288	arg1	effect					208:213	the effect	204:213	the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality	204:345	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	4	34	theme	resting	847:853	arg1	neutrophils					855:865	resting neutrophils	847:865	resting neutrophils	847:865	RESULTS There was not any influence of volume replacement solutions on the release of the enzymes from resting neutrophils.
24458309	5	35	theme	MMP-9	907:911	arg1	higher					917:922	higher	917:922	higher	917:922	After IL-8 stimulation, the release of MMP-9 was higher in BS than in UBS or RPMI-1640, whereas HES enhanced its release regardless of the composition.
24458309	5	35	theme	MMP-9	907:911	arg1	release					896:902	the release	892:902	the release of MMP-9	892:911	After IL-8 stimulation, the release of MMP-9 was higher in BS than in UBS or RPMI-1640, whereas HES enhanced its release regardless of the composition.
24458309	2	36	theme	gradient	493:500	arg1	centrifugation					502:515	gradient centrifugation	493:515	gradient centrifugation	493:515	MATERIALS AND METHODS Neutrophils were isolated by gradient centrifugation and dextran sedimentation and incubated in BS or UBS without or with HES, in the absence or presence of Interleukin-8 (IL-8) or Lipopolysaccharide (LPS).
24458309	2	37	dep	MATERIALS	442:450	arg1	Neutrophils					464:474	Neutrophils	464:474	Neutrophils	464:474	MATERIALS AND METHODS Neutrophils were isolated by gradient centrifugation and dextran sedimentation and incubated in BS or UBS without or with HES, in the absence or presence of Interleukin-8 (IL-8) or Lipopolysaccharide (LPS).
24458309	8	38	dep	CONCLUSION	1264:1273	arg1	solutions					1294:1302	Volume replacement solutions	1275:1302	CONCLUSION Volume replacement solutions	1264:1302	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	1	39	dep	unbalanced	236:245	arg1	UBS					248:250	UBS	248:250	UBS	248:250	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	0	40	dep	in	150:151	arg1	vitro					153:157	vitro	153:157	vitro	153:157	Balanced and unbalanced solutions modulate the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils in response to inflammatory stimuli: an in vitro study.
24458309	8	41	contain	have	1310:1313	arg2	impact					1318:1323	an impact	1315:1323	an impact on the release of MMP-9	1315:1347	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	8	41	contain	have	1310:1313	arg1	CONCLUSION					1264:1273	CONCLUSION	1264:1273	CONCLUSION Volume replacement solutions	1264:1302	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	1	42	from	effect	208:213	arg1	presence					281:288	presence	281:288	presence	281:288	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	1	42	from	effect	208:213	arg1	absence					270:276	the absence	266:276	the absence	266:276	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	1	42	from	effect	208:213	arg1	functionality					333:345	neutrophil functionality	322:345	neutrophil functionality	322:345	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	0	43	from	neutrophils	98:108	arg1	release					47:53	the release	43:53	the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils	43:108	Balanced and unbalanced solutions modulate the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils in response to inflammatory stimuli: an in vitro study.
24458309	5	44	theme	IL-8	874:877	arg1	stimulation					879:889	IL-8 stimulation	874:889	IL-8 stimulation	874:889	After IL-8 stimulation, the release of MMP-9 was higher in BS than in UBS or RPMI-1640, whereas HES enhanced its release regardless of the composition.
24458309	7	45	theme	MPO	1214:1216	arg1	release					1203:1209	the release	1199:1209	the release of MPO and MMP-8 after stimulation with IL-8 or LPS	1199:1261	No difference was found in the release of MPO and MMP-8 after stimulation with IL-8 or LPS.
24458309	8	46	theme	neutral	1456:1462	arg1	choice					1464:1469	a neutral choice	1454:1469	a neutral choice	1454:1469	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	8	46	theme	neutral	1456:1462	arg1	use					1407:1409	the use	1403:1409	the use of balanced or unbalanced solutions	1403:1445	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	6	47	theme	physiological	1146:1158	arg1	conditions					1160:1169	physiological conditions	1146:1169	physiological conditions	1146:1169	After LPS stimulation, the release of MMP-9 was higher in both UBS and BS than RPMI-1640, but HES brought its release back to physiological conditions.
24458309	0	48	theme	in	150:151	arg1	study					159:163	an in vitro study	147:163	inflammatory stimuli: an in vitro study	125:163	Balanced and unbalanced solutions modulate the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils in response to inflammatory stimuli: an in vitro study.
24458309	6	49	theme	MMP-9	1058:1062	arg1	release					1047:1053	the release	1043:1053	the release of MMP-9	1043:1062	After LPS stimulation, the release of MMP-9 was higher in both UBS and BS than RPMI-1640, but HES brought its release back to physiological conditions.
24458309	6	49	theme	MMP-9	1058:1062	arg1	higher					1068:1073	higher	1068:1073	higher	1068:1073	After LPS stimulation, the release of MMP-9 was higher in both UBS and BS than RPMI-1640, but HES brought its release back to physiological conditions.
24458309	1	50	theme	MMP-8	435:439	arg1	release					363:369	the release	359:369	the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8	359:439	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	8	51	theme	solutions	1437:1445	arg1	choice					1464:1469	a neutral choice	1454:1469	a neutral choice	1454:1469	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	8	51	theme	solutions	1437:1445	arg1	use					1407:1409	the use	1403:1409	the use of balanced or unbalanced solutions	1403:1445	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	2	52	dep	absence	598:604	arg1	the					594:596	the	594:596	the	594:596	MATERIALS AND METHODS Neutrophils were isolated by gradient centrifugation and dextran sedimentation and incubated in BS or UBS without or with HES, in the absence or presence of Interleukin-8 (IL-8) or Lipopolysaccharide (LPS).
24458309	7	53	located	found	1190:1194	arg1	release					1203:1209	the release	1199:1209	the release of MPO and MMP-8 after stimulation with IL-8 or LPS	1199:1261	No difference was found in the release of MPO and MMP-8 after stimulation with IL-8 or LPS.
24458309	7	53	located	found	1190:1194	arg2	difference					1175:1184	No difference	1172:1184	No difference	1172:1184	No difference was found in the release of MPO and MMP-8 after stimulation with IL-8 or LPS.
24458309	0	54	theme	Metalloproteinase-9	65:83	arg1	release					47:53	the release	43:53	the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils	43:108	Balanced and unbalanced solutions modulate the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils in response to inflammatory stimuli: an in vitro study.
24458309	1	55	theme	matrix	374:379	arg1	MMP					400:402	MMP	400:402	MMP	400:402	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	1	55	theme	matrix	374:379	arg1	metalloproteinase					381:397	matrix metalloproteinase (MMP)-9	374:405	matrix metalloproteinase (MMP)-9	374:405	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	0	56	dep	stimuli	138:144	arg1	study					159:163	an in vitro study	147:163	inflammatory stimuli: an in vitro study	125:163	Balanced and unbalanced solutions modulate the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils in response to inflammatory stimuli: an in vitro study.
24458309	1	57	theme	balanced	218:225	arg1	solutions					253:261	balanced (BS) and unbalanced (UBS) solutions	218:261	balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES)	218:317	OBJECTIVES AND DESIGN We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in the absence or presence of hydroxyethyl starch (HES) on neutrophil functionality, evaluating the release of matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), and MMP-8.
24458309	0	58	theme	Matrix	58:63	arg1	MMP-9					86:90	MMP-9	86:90	MMP-9	86:90	Balanced and unbalanced solutions modulate the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils in response to inflammatory stimuli: an in vitro study.
24458309	0	58	theme	Matrix	58:63	arg1	Metalloproteinase-9					65:83	Matrix Metalloproteinase-9	58:83	Matrix Metalloproteinase-9 (MMP-9)	58:91	Balanced and unbalanced solutions modulate the release of Matrix Metalloproteinase-9 (MMP-9) from neutrophils in response to inflammatory stimuli: an in vitro study.
24458309	7	59	with	stimulation	1234:1244	arg1	IL-8					1251:1254	IL-8	1251:1254	IL-8	1251:1254	No difference was found in the release of MPO and MMP-8 after stimulation with IL-8 or LPS.
24458309	7	59	with	stimulation	1234:1244	arg1	LPS					1259:1261	LPS	1259:1261	LPS	1259:1261	No difference was found in the release of MPO and MMP-8 after stimulation with IL-8 or LPS.
24458309	8	60	theme	balanced	1414:1421	arg1	solutions					1437:1445	balanced or unbalanced solutions	1414:1445	balanced or unbalanced solutions	1414:1445	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24458309	8	61	from	impact	1318:1323	arg1	release					1332:1338	the release	1328:1338	the release of MMP-9	1328:1347	CONCLUSION Volume replacement solutions might have an impact on the release of MMP-9 depending on the inflammatory milieu, suggesting that the use of balanced or unbalanced solutions is not a neutral choice.
24527488	4	0	used	used	568:571	arg2	model					630:634	a model	628:634	a model of target analyte	628:652	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	4	0	used	used	568:571	arg2	gene					620:623	p53 tumor suppressor gene	599:623	p53 tumor suppressor gene	599:623	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	4	0	used	used	568:571	arg2	Oxalate					557:563	Oxalate	557:563	Oxalate	557:563	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	4	0	used	used	568:571	arg2	co-reactant					583:593	an ECL co-reactant	576:593	an ECL co-reactant	576:593	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	3	1	theme	DNA	508:510	arg1	probe					512:516	DNA probe	508:516	DNA probe	508:516	Here, a simple and label-free electrochemiluminescence (ECL) method for sensing the recognition event of DNA probe to sequence-specific DNA is developed.
24527488	12	2	theme	×	1798:1798	arg1	L					1811:1811	1.0 × 10(-9) mol L(-1)	1794:1815	1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1)	1794:1862	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	6	3	theme	DNA	830:832	arg1	probes					834:839	DNA probes	830:839	DNA probes	830:839	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	9	4	theme	duplex	1491:1496	arg1	DNA					1498:1500	the duplex DNA	1487:1500	the duplex DNA	1487:1500	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	4	5	theme	target	639:644	arg1	analyte					646:652	target analyte	639:652	target analyte	639:652	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	1	6	theme	DNA	186:188	arg1	probes					190:195	the functional DNA probes	171:195	the functional DNA probes	171:195	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	1	6	theme	DNA	186:188	arg1	elements					161:168	the powerful molecular recognition elements	126:168	the powerful molecular recognition elements	126:168	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	1	6	theme	DNA	186:188	arg1	one					119:121	one	119:121	one	119:121	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	12	7	theme	L	1858:1858	arg1	limit					1831:1835	limit	1831:1835	a detect limit of 2.7 × 10(-12) mol L(-1)	1822:1862	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	6	8	from	embedded	987:994	arg1	film					1017:1020	the ECL composite film	999:1020	the ECL composite film	999:1020	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	2	9	theme	DNA	320:322	arg1	probe					324:328	DNA probe	320:328	DNA probe binding to the specific target	320:359	However, the accurate readout of the recognition event of DNA probe binding to the specific target by label-free means is still challenging.
24527488	9	10	theme	target	1318:1323	arg1	sequences					1325:1333	target sequences	1318:1333	target sequences	1318:1333	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	6	11	theme	composite	896:904	arg1	electrode					911:919	the nanochannel-based composite film electrode	874:919	the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film	874:1020	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	12	12	theme	×	1844:1844	arg1	-1					1860:1861	-1	1860:1861	-1	1860:1861	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	12	12	theme	×	1844:1844	arg1	L					1858:1858	2.7 × 10(-12) mol L	1840:1858	2.7 × 10(-12) mol L(-1)	1840:1862	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	0	13	dep	capped	91:96	arg1	ion					98:100	ion	98:100	ion	98:100	Label-free electrochemiluminescence detection of specific-sequence DNA based on DNA probes capped ion nanochannels.
24527488	0	13	dep	capped	91:96	arg1	probes					84:89	DNA probes	80:89	DNA probes capped ion nanochannels	80:113	Label-free electrochemiluminescence detection of specific-sequence DNA based on DNA probes capped ion nanochannels.
24527488	0	13	dep	capped	91:96	arg1	nanochannels					102:113	nanochannels	102:113	nanochannels	102:113	Label-free electrochemiluminescence detection of specific-sequence DNA based on DNA probes capped ion nanochannels.
24527488	11	14	theme	ECL	1609:1611	arg1	method					1613:1618	this ECL method	1604:1618	this ECL method	1604:1618	Our results show that this ECL method could effectively discriminate complementary from single-base mismatch DNA sequences.
24527488	12	15	with	L	1811:1811	arg1	limit					1831:1835	limit	1831:1835	a detect limit of 2.7 × 10(-12) mol L(-1)	1822:1862	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	2	16	theme	event	311:315	arg1	readout					284:290	the accurate readout	271:290	the accurate readout of the recognition event of DNA probe binding to the specific target by label-free means	271:379	However, the accurate readout of the recognition event of DNA probe binding to the specific target by label-free means is still challenging.
24527488	8	17	theme	nanochannel-based	1153:1169	arg1	film					1181:1184	the nanochannel-based composite film	1149:1184	the nanochannel-based composite film	1149:1184	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	5	18	theme	nanochannel	688:698	arg1	film					711:714	the nanochannel structural film	684:714	the nanochannel structural film	684:714	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	9	19	theme	hybridizing	1340:1350	arg1	reaction					1352:1359	the hybridizing reaction	1336:1359	the hybridizing reaction of targets with DNA probes	1336:1386	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	5	20	theme	sensing	666:672	arg1	platform					674:681	the ECL sensing platform	658:681	the ECL sensing platform	658:681	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	1	21	theme	molecular	139:147	arg1	elements					161:168	the powerful molecular recognition elements	126:168	the powerful molecular recognition elements	126:168	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	1	22	theme	powerful	130:137	arg1	elements					161:168	the powerful molecular recognition elements	126:168	the powerful molecular recognition elements	126:168	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	0	23	theme	DNA	80:82	arg1	ion					98:100	ion	98:100	ion	98:100	Label-free electrochemiluminescence detection of specific-sequence DNA based on DNA probes capped ion nanochannels.
24527488	0	23	theme	DNA	80:82	arg1	probes					84:89	DNA probes	80:89	DNA probes capped ion nanochannels	80:113	Label-free electrochemiluminescence detection of specific-sequence DNA based on DNA probes capped ion nanochannels.
24527488	0	23	theme	DNA	80:82	arg1	nanochannels					102:113	nanochannels	102:113	nanochannels	102:113	Label-free electrochemiluminescence detection of specific-sequence DNA based on DNA probes capped ion nanochannels.
24527488	1	24	theme	elements	161:168	arg1	probes					190:195	the functional DNA probes	171:195	the functional DNA probes	171:195	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	1	24	theme	elements	161:168	arg1	elements					161:168	the powerful molecular recognition elements	126:168	the powerful molecular recognition elements	126:168	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	1	24	theme	elements	161:168	arg1	one					119:121	one	119:121	one	119:121	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	6	25	theme	stronger	934:941	arg1	interaction					943:953	the stronger interaction	930:953	the stronger interaction between DNA probes and chitosan embedded in the ECL composite film	930:1020	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	5	26	contain	contains	723:730	arg2	chitosan					744:751	chitosan	744:751	chitosan	744:751	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	5	26	contain	contains	723:730	arg2	2+					766:767	2+	766:767	2+	766:767	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	5	26	contain	contains	723:730	arg2	silica-sol					732:741	silica-sol	732:741	silica-sol	732:741	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	5	26	contain	contains	723:730	arg2	bpy					760:762	Ru(bpy)3	757:764	Ru(bpy)3(2+)	757:768	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	5	26	contain	contains	723:730	arg1	film					711:714	the nanochannel structural film	684:714	the nanochannel structural film	684:714	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	8	27	from	film	1181:1184	arg1	solution					1222:1229	the bulk solution	1213:1229	the bulk solution	1213:1229	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	8	27	from	film	1181:1184	arg1	embedded					1137:1144	embedded	1137:1144	embedded	1137:1144	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	6	28	theme	electrode	911:919	arg1	surface					863:869	the surface	859:869	the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film	859:1020	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	3	29	theme	electrochemiluminescence	433:456	arg1	method					464:469	a simple and label-free electrochemiluminescence (ECL) method	409:469	a simple and label-free electrochemiluminescence (ECL) method for sensing the recognition event of DNA probe to sequence-specific DNA	409:541	Here, a simple and label-free electrochemiluminescence (ECL) method for sensing the recognition event of DNA probe to sequence-specific DNA is developed.
24527488	6	30	theme	ECL	1003:1005	arg1	film					1017:1020	the ECL composite film	999:1020	the ECL composite film	999:1020	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	9	31	with	reaction	1352:1359	arg1	probes					1381:1386	DNA probes	1377:1386	DNA probes	1377:1386	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	0	32	theme	Label-free	0:9	arg1	detection					36:44	Label-free electrochemiluminescence detection	0:44	Label-free electrochemiluminescence detection of specific-sequence DNA	0:69	Label-free electrochemiluminescence detection of specific-sequence DNA based on DNA probes capped ion nanochannels.
24527488	13	33	theme	silica-chitosan	1918:1932	arg1	film					1944:1947	the silica-chitosan composite film	1914:1947	the silica-chitosan composite film	1914:1947	This work demonstrates that porous structures on the silica-chitosan composite film can provide a label-free and general platform to measure the change of DNA configuration.
24527488	12	34	theme	linear	1740:1745	arg1	range					1747:1751	the linear range	1736:1751	the linear range for target DNA	1736:1766	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	8	35	from	co-reactant	1198:1208	arg1	solution					1222:1229	the bulk solution	1213:1229	the bulk solution	1213:1229	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	8	35	from	co-reactant	1198:1208	arg1	embedded					1137:1144	embedded	1137:1144	embedded	1137:1144	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	9	36	theme	DNA	1422:1424	arg1	probes					1426:1431	the DNA probes	1418:1431	the DNA probes	1418:1431	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	9	37	from	film	1452:1455	arg1	escape					1408:1413	the escape	1404:1413	the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA	1404:1500	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	8	38	theme	weak	1257:1260	arg1	signal					1266:1271	a weak ECL signal	1255:1271	a weak ECL signal	1255:1271	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	1	39	theme	recognition	149:159	arg1	elements					161:168	the powerful molecular recognition elements	126:168	the powerful molecular recognition elements	126:168	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	0	40	theme	specific-sequence	49:65	arg1	DNA					67:69	specific-sequence DNA	49:69	specific-sequence DNA	49:69	Label-free electrochemiluminescence detection of specific-sequence DNA based on DNA probes capped ion nanochannels.
24527488	13	41	theme	porous	1893:1898	arg1	structures					1900:1909	porous structures	1893:1909	porous structures on the silica-chitosan composite film	1893:1947	This work demonstrates that porous structures on the silica-chitosan composite film can provide a label-free and general platform to measure the change of DNA configuration.
24527488	5	42	theme	Ru	757:758	arg1	bpy					760:762	Ru(bpy)3	757:764	Ru(bpy)3(2+)	757:768	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	5	42	theme	Ru	757:758	arg1	2+					766:767	2+	766:767	2+	766:767	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	9	43	theme	composite	1442:1450	arg1	film					1452:1455	the composite film	1438:1455	the composite film due to the rigid structure of the duplex DNA	1438:1500	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	3	44	theme	recognition	487:497	arg1	event					499:503	the recognition event	483:503	the recognition event of DNA probe to sequence-specific DNA	483:541	Here, a simple and label-free electrochemiluminescence (ECL) method for sensing the recognition event of DNA probe to sequence-specific DNA is developed.
24527488	12	45	theme	optimal	1716:1722	arg1	conditions					1724:1733	the optimal conditions	1712:1733	the optimal conditions	1712:1733	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	9	46	theme	due	1457:1459	arg1	film					1452:1455	the composite film	1438:1455	the composite film due to the rigid structure of the duplex DNA	1438:1500	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	11	47	theme	mismatch	1682:1689	arg1	sequences					1695:1703	single-base mismatch DNA sequences	1670:1703	single-base mismatch DNA sequences	1670:1703	Our results show that this ECL method could effectively discriminate complementary from single-base mismatch DNA sequences.
24527488	9	48	dep	sequences	1325:1333	arg1	the					1302:1304	the	1302:1304	the	1302:1304	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	9	48	dep	sequences	1325:1333	arg1	presence					1306:1313	presence	1306:1313	presence	1306:1313	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	6	49	from	film	1017:1020	arg1	embedded					987:994	embedded	987:994	embedded	987:994	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	3	50	theme	probe	512:516	arg1	event					499:503	the recognition event	483:503	the recognition event of DNA probe to sequence-specific DNA	483:541	Here, a simple and label-free electrochemiluminescence (ECL) method for sensing the recognition event of DNA probe to sequence-specific DNA is developed.
24527488	4	51	theme	tumor	603:607	arg1	Oxalate					557:563	Oxalate	557:563	Oxalate	557:563	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	4	51	theme	tumor	603:607	arg1	gene					620:623	p53 tumor suppressor gene	599:623	p53 tumor suppressor gene	599:623	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	10	52	theme	stronger	1548:1555	arg1	signal					1561:1566	a stronger ECL signal	1546:1566	a stronger ECL signal	1546:1566	Thus, these nanochannels were uncapped and a stronger ECL signal was detected.
24527488	12	53	theme	mol	1807:1809	arg1	L					1811:1811	1.0 × 10(-9) mol L(-1)	1794:1815	1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1)	1794:1862	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	2	54	theme	recognition	299:309	arg1	event					311:315	the recognition event	295:315	the recognition event of DNA probe binding to the specific target	295:359	However, the accurate readout of the recognition event of DNA probe binding to the specific target by label-free means is still challenging.
24527488	9	55	theme	DNA	1498:1500	arg1	structure					1474:1482	the rigid structure	1464:1482	the rigid structure of the duplex DNA	1464:1500	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	4	56	theme	ECL	579:581	arg1	Oxalate					557:563	Oxalate	557:563	Oxalate	557:563	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	4	56	theme	ECL	579:581	arg1	co-reactant					583:593	an ECL co-reactant	576:593	an ECL co-reactant	576:593	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	2	57	theme	specific	345:352	arg1	target					354:359	the specific target	341:359	the specific target	341:359	However, the accurate readout of the recognition event of DNA probe binding to the specific target by label-free means is still challenging.
24527488	3	58	theme	sequence-specific	521:537	arg1	DNA					539:541	sequence-specific DNA	521:541	sequence-specific DNA	521:541	Here, a simple and label-free electrochemiluminescence (ECL) method for sensing the recognition event of DNA probe to sequence-specific DNA is developed.
24527488	1	59	theme	functional	175:184	arg1	probes					190:195	the functional DNA probes	171:195	the functional DNA probes	171:195	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	1	59	theme	functional	175:184	arg1	elements					161:168	the powerful molecular recognition elements	126:168	the powerful molecular recognition elements	126:168	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	1	59	theme	functional	175:184	arg1	one					119:121	one	119:121	one	119:121	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	13	60	theme	configuration	2024:2036	arg1	change					2010:2015	the change	2006:2015	the change of DNA configuration	2006:2036	This work demonstrates that porous structures on the silica-chitosan composite film can provide a label-free and general platform to measure the change of DNA configuration.
24527488	2	61	theme	probe	324:328	arg1	event					311:315	the recognition event	295:315	the recognition event of DNA probe binding to the specific target	295:359	However, the accurate readout of the recognition event of DNA probe binding to the specific target by label-free means is still challenging.
24527488	5	62	theme	electrochemical	789:803	arg1	method					816:821	an electrochemical deposition method	786:821	an electrochemical deposition method	786:821	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	8	63	theme	mass-transfer	1090:1102	arg1	result					1078:1083	a result	1076:1083	a result	1076:1083	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	8	63	theme	mass-transfer	1090:1102	arg1	channel					1104:1110	the mass-transfer channel	1086:1110	the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution	1086:1229	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	6	64	theme	film	906:909	arg1	electrode					911:919	the nanochannel-based composite film electrode	874:919	the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film	874:1020	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	4	65	theme	analyte	646:652	arg1	Oxalate					557:563	Oxalate	557:563	Oxalate	557:563	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	4	65	theme	analyte	646:652	arg1	model					630:634	a model	628:634	a model of target analyte	628:652	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	6	66	theme	nanochannel-based	878:894	arg1	electrode					911:919	the nanochannel-based composite film electrode	874:919	the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film	874:1020	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	8	67	theme	embedded	1137:1144	arg1	2+					1133:1134	2+	1133:1134	2+	1133:1134	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	8	67	theme	embedded	1137:1144	arg1	bpy					1127:1129	the Ru(bpy)3	1120:1131	the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution	1120:1229	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	5	68	theme	deposition	805:814	arg1	method					816:821	an electrochemical deposition method	786:821	an electrochemical deposition method	786:821	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	8	69	theme	Ru	1124:1125	arg1	2+					1133:1134	2+	1133:1134	2+	1133:1134	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	8	69	theme	Ru	1124:1125	arg1	bpy					1127:1129	the Ru(bpy)3	1120:1131	the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution	1120:1229	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	1	70	used	utilized	220:227	arg2	one					119:121	one	119:121	one	119:121	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	1	70	used	utilized	220:227	arg2	elements					161:168	the powerful molecular recognition elements	126:168	the powerful molecular recognition elements	126:168	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	1	70	used	utilized	220:227	arg2	probes					190:195	the functional DNA probes	171:195	the functional DNA probes	171:195	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	11	71	theme	single-base	1670:1680	arg1	sequences					1695:1703	single-base mismatch DNA sequences	1670:1703	single-base mismatch DNA sequences	1670:1703	Our results show that this ECL method could effectively discriminate complementary from single-base mismatch DNA sequences.
24527488	13	72	theme	general	1978:1984	arg1	platform					1986:1993	a label-free and general platform	1961:1993	a label-free and general platform to measure the change of DNA configuration	1961:2036	This work demonstrates that porous structures on the silica-chitosan composite film can provide a label-free and general platform to measure the change of DNA configuration.
24527488	8	73	theme	composite	1171:1179	arg1	film					1181:1184	the nanochannel-based composite film	1149:1184	the nanochannel-based composite film	1149:1184	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	5	74	theme	ECL	662:664	arg1	platform					674:681	the ECL sensing platform	658:681	the ECL sensing platform	658:681	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	2	75	theme	accurate	275:282	arg1	readout					284:290	the accurate readout	271:290	the accurate readout of the recognition event of DNA probe binding to the specific target by label-free means	271:379	However, the accurate readout of the recognition event of DNA probe binding to the specific target by label-free means is still challenging.
24527488	9	76	theme	targets	1364:1370	arg1	reaction					1352:1359	the hybridizing reaction	1336:1359	the hybridizing reaction of targets with DNA probes	1336:1386	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	6	77	theme	DNA	963:965	arg1	probes					967:972	DNA probes	963:972	DNA probes	963:972	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	9	78	theme	DNA	1377:1379	arg1	probes					1381:1386	DNA probes	1377:1386	DNA probes	1377:1386	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	8	79	theme	ECL	1194:1196	arg1	co-reactant					1198:1208	the ECL co-reactant	1190:1208	the ECL co-reactant in the bulk solution	1190:1229	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	12	80	dep	limit	1831:1835	arg1	detect					1824:1829	detect	1824:1829	detect	1824:1829	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	12	81	theme	target	1757:1762	arg1	DNA					1764:1766	target DNA	1757:1766	target DNA	1757:1766	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	8	82	from	embedded	1137:1144	arg1	co-reactant					1198:1208	the ECL co-reactant	1190:1208	the ECL co-reactant in the bulk solution	1190:1229	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	8	82	from	embedded	1137:1144	arg1	film					1181:1184	the nanochannel-based composite film	1149:1184	the nanochannel-based composite film	1149:1184	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	13	83	theme	label-free	1963:1972	arg1	platform					1986:1993	a label-free and general platform	1961:1993	a label-free and general platform to measure the change of DNA configuration	1961:2036	This work demonstrates that porous structures on the silica-chitosan composite film can provide a label-free and general platform to measure the change of DNA configuration.
24527488	5	84	theme	structural	700:709	arg1	film					711:714	the nanochannel structural film	684:714	the nanochannel structural film	684:714	In the ECL sensing platform, the nanochannel structural film, which contains silica-sol, chitosan and Ru(bpy)3(2+), is prepared by an electrochemical deposition method.
24527488	13	85	from	structures	1900:1909	arg1	film					1944:1947	the silica-chitosan composite film	1914:1947	the silica-chitosan composite film	1914:1947	This work demonstrates that porous structures on the silica-chitosan composite film can provide a label-free and general platform to measure the change of DNA configuration.
24527488	8	86	theme	bulk	1217:1220	arg1	solution					1222:1229	the bulk solution	1213:1229	the bulk solution	1213:1229	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	11	87	theme	DNA	1691:1693	arg1	sequences					1695:1703	single-base mismatch DNA sequences	1670:1703	single-base mismatch DNA sequences	1670:1703	Our results show that this ECL method could effectively discriminate complementary from single-base mismatch DNA sequences.
24527488	6	88	theme	composite	1007:1015	arg1	film					1017:1020	the ECL composite film	999:1020	the ECL composite film	999:1020	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	0	89	theme	electrochemiluminescence	11:34	arg1	detection					36:44	Label-free electrochemiluminescence detection	0:44	Label-free electrochemiluminescence detection of specific-sequence DNA	0:69	Label-free electrochemiluminescence detection of specific-sequence DNA based on DNA probes capped ion nanochannels.
24527488	3	90	theme	label-free	422:431	arg1	method					464:469	a simple and label-free electrochemiluminescence (ECL) method	409:469	a simple and label-free electrochemiluminescence (ECL) method for sensing the recognition event of DNA probe to sequence-specific DNA	409:541	Here, a simple and label-free electrochemiluminescence (ECL) method for sensing the recognition event of DNA probe to sequence-specific DNA is developed.
24527488	13	91	theme	composite	1934:1942	arg1	film					1944:1947	the silica-chitosan composite film	1914:1947	the silica-chitosan composite film	1914:1947	This work demonstrates that porous structures on the silica-chitosan composite film can provide a label-free and general platform to measure the change of DNA configuration.
24527488	9	92	theme	probes	1426:1431	arg1	escape					1408:1413	the escape	1404:1413	the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA	1404:1500	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	6	93	theme	embedded	987:994	arg1	probes					967:972	DNA probes	963:972	DNA probes	963:972	Then, DNA probes are attached onto the surface of the nanochannel-based composite film electrode based on the stronger interaction between DNA probes and chitosan embedded in the ECL composite film.
24527488	12	94	theme	mol	1854:1856	arg1	-1					1860:1861	-1	1860:1861	-1	1860:1861	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	12	94	theme	mol	1854:1856	arg1	L					1858:1858	2.7 × 10(-12) mol L	1840:1858	2.7 × 10(-12) mol L(-1)	1840:1862	Under the optimal conditions, the linear range for target DNA was from 1.0 × 10(-11) to 1.0 × 10(-9) mol L(-1) with a detect limit of 2.7 × 10(-12) mol L(-1).
24527488	0	95	theme	DNA	67:69	arg1	detection					36:44	Label-free electrochemiluminescence detection	0:44	Label-free electrochemiluminescence detection of specific-sequence DNA	0:69	Label-free electrochemiluminescence detection of specific-sequence DNA based on DNA probes capped ion nanochannels.
24527488	3	96	theme	simple	411:416	arg1	method					464:469	a simple and label-free electrochemiluminescence (ECL) method	409:469	a simple and label-free electrochemiluminescence (ECL) method for sensing the recognition event of DNA probe to sequence-specific DNA	409:541	Here, a simple and label-free electrochemiluminescence (ECL) method for sensing the recognition event of DNA probe to sequence-specific DNA is developed.
24527488	8	97	theme	ECL	1262:1264	arg1	signal					1266:1271	a weak ECL signal	1255:1271	a weak ECL signal	1255:1271	As a result, the mass-transfer channel between the Ru(bpy)3(2+) embedded in the nanochannel-based composite film and the ECL co-reactant in the bulk solution was greatly blocked and a weak ECL signal was observed.
24527488	7	98	theme	DNA	1061:1063	arg1	probes					1065:1070	the DNA probes	1057:1070	the DNA probes	1057:1070	These nanochannels were capped by the DNA probes.
24527488	4	99	theme	p53	599:601	arg1	Oxalate					557:563	Oxalate	557:563	Oxalate	557:563	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	4	99	theme	p53	599:601	arg1	gene					620:623	p53 tumor suppressor gene	599:623	p53 tumor suppressor gene	599:623	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	2	100	theme	label-free	364:373	arg1	means					375:379	label-free means	364:379	label-free means	364:379	However, the accurate readout of the recognition event of DNA probe binding to the specific target by label-free means is still challenging.
24527488	1	101	theme	various	242:248	arg1	biosensors					250:259	various biosensors	242:259	various biosensors	242:259	As one of the powerful molecular recognition elements, the functional DNA probes have been successfully utilized to construct various biosensors.
24527488	13	102	theme	DNA	2020:2022	arg1	configuration					2024:2036	DNA configuration	2020:2036	DNA configuration	2020:2036	This work demonstrates that porous structures on the silica-chitosan composite film can provide a label-free and general platform to measure the change of DNA configuration.
24527488	9	103	theme	rigid	1468:1472	arg1	structure					1474:1482	the rigid structure	1464:1482	the rigid structure of the duplex DNA	1464:1500	Conversely, in the presence of target sequences, the hybridizing reaction of targets with DNA probes could result in the escape of the DNA probes from the composite film due to the rigid structure of the duplex DNA.
24527488	4	104	theme	suppressor	609:618	arg1	Oxalate					557:563	Oxalate	557:563	Oxalate	557:563	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	4	104	theme	suppressor	609:618	arg1	gene					620:623	p53 tumor suppressor gene	599:623	p53 tumor suppressor gene	599:623	Oxalate is used as an ECL co-reactant and p53 tumor suppressor gene as a model of target analyte.
24527488	10	105	theme	ECL	1557:1559	arg1	signal					1561:1566	a stronger ECL signal	1546:1566	a stronger ECL signal	1546:1566	Thus, these nanochannels were uncapped and a stronger ECL signal was detected.
27217558	4	0	from	present	692:698	arg1	spaces					721:726	the extracellular spaces	703:726	the extracellular spaces of plant cells	703:741	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	6	1	theme	mechanical	1201:1210	arg1	interlocking					1212:1223	mechanical interlocking	1201:1223	mechanical interlocking between the adventitious roots of English ivy and the surface of substrates	1201:1299	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	1	2	theme	yellowish	241:249	arg1	mucilage					251:258	a yellowish mucilage	239:258	a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces	239:326	Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces.
27217558	7	3	theme	calcium	1482:1488	arg1	ions					1490:1493	calcium ions	1482:1493	calcium ions	1482:1493	Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions.
27217558	7	4	theme	AGP-rich	1423:1430	arg1	nanoparticles					1436:1448	purified AGP-rich ivy nanoparticles	1414:1448	purified AGP-rich ivy nanoparticles	1414:1448	Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions.
27217558	3	5	theme	viscous	523:529	arg1	exudate					531:537	the viscous exudate	519:537	the viscous exudate	519:537	In the previous studies, spherical nanoparticles were observed in the viscous exudate.
27217558	4	6	theme	hydroxyproline-rich	658:676	arg1	glycoproteins					678:690	hydroxyproline-rich glycoproteins	658:690	hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells	658:741	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	5	7	theme	favorable	858:866	arg1	behavior					876:883	the ivy adhesive, and thus a favorable wetting behavior	829:883	behavior	876:883	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	6	8	theme	carboxyl	974:981	arg1	AGPs					997:1000	the AGPs	993:1000	the AGPs	993:1000	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	6	8	theme	carboxyl	974:981	arg1	groups					983:988	carboxyl groups	974:988	carboxyl groups of the AGPs	974:1000	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	9	9	theme	other	1730:1734	arg1	plants					1745:1750	other climbing plants	1730:1750	other climbing plants	1730:1750	Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
27217558	7	10	theme	adhesive	1366:1373	arg1	composite					1375:1383	a reconstructed ivy-mimetic adhesive composite	1338:1383	a reconstructed ivy-mimetic adhesive composite	1338:1383	Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions.
27217558	5	11	theme	ivy	833:835	arg1	viscosity					816:824	a low viscosity	810:824	a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates	810:912	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	1	12	theme	English	206:212	arg1	ivy					214:216	English ivy	206:216	English ivy (Hedera helix)	206:231	Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces.
27217558	1	12	theme	English	206:212	arg1	helix					226:230	Hedera helix	219:230	Hedera helix	219:230	Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces.
27217558	6	13	theme	calcium-driven	926:939	arg1	interactions					955:966	calcium-driven electrostatic interactions	926:966	calcium-driven electrostatic interactions among carboxyl groups of the AGPs	926:1000	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	7	14	theme	ivy-mimetic	1354:1364	arg1	composite					1375:1383	a reconstructed ivy-mimetic adhesive composite	1338:1383	a reconstructed ivy-mimetic adhesive composite	1338:1383	Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions.
27217558	6	15	theme	substrates	1290:1299	arg1	roots					1250:1254	the adventitious roots	1233:1254	the adventitious roots of English ivy	1233:1269	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	6	15	theme	substrates	1290:1299	arg1	surface					1279:1285	the surface	1275:1285	the surface of substrates	1275:1299	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	4	16	theme	plant	731:735	arg1	cells					737:741	plant cells	731:741	plant cells	731:741	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	4	17	theme	present	692:698	arg1	glycoproteins					678:690	hydroxyproline-rich glycoproteins	658:690	hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells	658:741	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	5	18	theme	behavior	876:883	arg1	viscosity					816:824	a low viscosity	810:824	a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates	810:912	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	0	19	theme	adhesive	80:87	arg1	proteins					30:37	Nanospherical arabinogalactan proteins	0:37	Nanospherical arabinogalactan proteins	0:37	Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy.
27217558	0	19	theme	adhesive	80:87	arg1	component					49:57	a key component	43:57	a key component of the high-strength adhesive secreted by English ivy	43:111	Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy.
27217558	1	20	theme	vertical	310:317	arg1	surfaces					319:326	climb vertical surfaces	304:326	climb vertical surfaces	304:326	Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces.
27217558	6	21	theme	AGPs	997:1000	arg1	AGPs					997:1000	the AGPs	993:1000	the AGPs	993:1000	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	6	21	theme	AGPs	997:1000	arg1	groups					983:988	carboxyl groups	974:988	carboxyl groups of the AGPs	974:1000	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	8	22	theme	subsequent	1524:1533	arg1	tests					1543:1547	the subsequent tensile tests	1520:1547	the subsequent tensile tests	1520:1547	Information gained from the subsequent tensile tests, in turn, substantiated the proposed adhesion mechanisms underlying the ivy-derived adhesive.
27217558	5	23	from	viscosity	816:824	arg1	surface					892:898	the surface	888:898	the surface of substrates	888:912	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	5	24	theme	spheroidal	748:757	arg1	shape					759:763	The spheroidal shape	744:763	The spheroidal shape of the AGP-rich ivy nanoparticles	744:797	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	0	25	theme	key	45:47	arg1	proteins					30:37	Nanospherical arabinogalactan proteins	0:37	Nanospherical arabinogalactan proteins	0:37	Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy.
27217558	0	25	theme	key	45:47	arg1	component					49:57	a key component	43:57	a key component of the high-strength adhesive secreted by English ivy	43:111	Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy.
27217558	6	26	theme	exuded	1061:1066	arg1	substances					1077:1086	the exuded adhesive substances	1057:1086	the exuded adhesive substances	1057:1086	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	0	27	theme	Nanospherical	0:12	arg1	proteins					30:37	Nanospherical arabinogalactan proteins	0:37	Nanospherical arabinogalactan proteins	0:37	Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy.
27217558	0	27	theme	Nanospherical	0:12	arg1	component					49:57	a key component	43:57	a key component of the high-strength adhesive secreted by English ivy	43:111	Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy.
27217558	5	28	from	surface	892:898	arg1	viscosity					816:824	a low viscosity	810:824	a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates	810:912	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	9	29	theme	general	1851:1857	arg1	principles					1859:1868	the general principles	1847:1868	the general principles underlying diverse botanic adhesives	1847:1905	Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
27217558	2	30	theme	adhesion	393:400	arg1	mechanisms					402:411	the adhesion mechanisms	389:411	the adhesion mechanisms underlying this high-strength adhesive	389:450	Unfortunately, little progress has been made in elucidating the adhesion mechanisms underlying this high-strength adhesive.
27217558	6	31	theme	film	1154:1157	arg1	formation					1129:1137	formation	1129:1137	formation of an adhesive film	1129:1157	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	5	32	theme	nanoparticles	785:797	arg1	shape					759:763	The spheroidal shape	744:763	The spheroidal shape of the AGP-rich ivy nanoparticles	744:797	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	9	33	theme	botanic	1889:1895	arg1	adhesives					1897:1905	diverse botanic adhesives	1881:1905	diverse botanic adhesives	1881:1905	Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
27217558	8	34	theme	adhesion	1586:1593	arg1	mechanisms					1595:1604	the proposed adhesion mechanisms	1573:1604	the proposed adhesion mechanisms underlying the ivy-derived adhesive	1573:1640	Information gained from the subsequent tensile tests, in turn, substantiated the proposed adhesion mechanisms underlying the ivy-derived adhesive.
27217558	8	35	from	tests	1543:1547	arg1	turn					1553:1556	turn	1553:1556	turn	1553:1556	Information gained from the subsequent tensile tests, in turn, substantiated the proposed adhesion mechanisms underlying the ivy-derived adhesive.
27217558	3	36	theme	previous	460:467	arg1	studies					469:475	the previous studies	456:475	the previous studies	456:475	In the previous studies, spherical nanoparticles were observed in the viscous exudate.
27217558	1	37	theme	ivy	214:216	arg1	roots					197:201	the adventitious roots	180:201	the adventitious roots of English ivy (Hedera helix)	180:231	Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces.
27217558	6	38	theme	adhesive	1145:1152	arg1	film					1154:1157	an adhesive film	1142:1157	an adhesive film	1142:1157	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	2	39	theme	little	344:349	arg1	progress					351:358	little progress	344:358	little progress	344:358	Unfortunately, little progress has been made in elucidating the adhesion mechanisms underlying this high-strength adhesive.
27217558	1	40	theme	Hedera	219:224	arg1	ivy					214:216	English ivy	206:216	English ivy (Hedera helix)	206:231	Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces.
27217558	1	40	theme	Hedera	219:224	arg1	helix					226:230	Hedera helix	219:230	Hedera helix	219:230	Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces.
27217558	9	41	theme	pectic	1663:1668	arg1	polysaccharides					1670:1684	pectic polysaccharides	1663:1684	pectic polysaccharides	1663:1684	Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
27217558	4	42	theme	arabinogalactan	608:622	arg1	superfamily					643:653	a superfamily	641:653	a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells	641:741	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	4	42	theme	arabinogalactan	608:622	arg1	AGPs					634:637	AGPs	634:637	AGPs	634:637	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	4	42	theme	arabinogalactan	608:622	arg1	proteins					624:631	arabinogalactan proteins	608:631	arabinogalactan proteins (AGPs)	608:638	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	6	43	theme	substances	1077:1086	arg1	cross-linking					1040:1052	the cross-linking	1036:1052	the cross-linking of the exuded adhesive substances	1036:1086	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	6	44	theme	ivy	1267:1269	arg1	roots					1250:1254	the adventitious roots	1233:1254	the adventitious roots of English ivy	1233:1269	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	6	44	theme	ivy	1267:1269	arg1	surface					1279:1285	the surface	1275:1285	the surface of substrates	1275:1299	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	4	45	attach	present	692:698	arg2	glycoproteins					678:690	hydroxyproline-rich glycoproteins	658:690	hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells	658:741	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	4	45	attach	present	692:698	arg1	spaces					721:726	the extracellular spaces	703:726	the extracellular spaces of plant cells	703:741	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	7	46	theme	pectic	1455:1460	arg1	polysaccharides					1462:1476	pectic polysaccharides	1455:1476	pectic polysaccharides	1455:1476	Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions.
27217558	7	47	theme	ivy	1432:1434	arg1	nanoparticles					1436:1448	purified AGP-rich ivy nanoparticles	1414:1448	purified AGP-rich ivy nanoparticles	1414:1448	Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions.
27217558	5	48	theme	AGP-rich	772:779	arg1	nanoparticles					785:797	the AGP-rich ivy nanoparticles	768:797	the AGP-rich ivy nanoparticles	768:797	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	6	49	theme	adventitious	1237:1248	arg1	roots					1250:1254	the adventitious roots	1233:1254	the adventitious roots of English ivy	1233:1269	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	9	50	located	observed	1695:1702	arg1	bioadhesives					1707:1718	bioadhesives	1707:1718	bioadhesives exuded by other climbing plants	1707:1750	Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
27217558	9	50	located	observed	1695:1702	arg2	AGPs					1654:1657	AGPs	1654:1657	AGPs	1654:1657	Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
27217558	9	50	located	observed	1695:1702	arg2	polysaccharides					1670:1684	pectic polysaccharides	1663:1684	pectic polysaccharides	1663:1684	Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
27217558	7	51	theme	purified	1414:1421	arg1	nanoparticles					1436:1448	purified AGP-rich ivy nanoparticles	1414:1448	purified AGP-rich ivy nanoparticles	1414:1448	Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions.
27217558	1	52	theme	adventitious	184:195	arg1	roots					197:201	the adventitious roots	180:201	the adventitious roots of English ivy (Hedera helix)	180:231	Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces.
27217558	9	53	theme	climbing	1736:1743	arg1	plants					1745:1750	other climbing plants	1730:1750	other climbing plants	1730:1750	Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
27217558	5	54	theme	adhesive	837:844	arg1	ivy					833:835	the ivy adhesive, and thus a favorable wetting behavior	829:883	ivy	833:835	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	6	55	theme	electrostatic	941:953	arg1	interactions					955:966	calcium-driven electrostatic interactions	926:966	calcium-driven electrostatic interactions among carboxyl groups of the AGPs	926:1000	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	6	56	theme	interlocking	1212:1223	arg1	generation					1187:1196	the generation	1183:1196	the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates	1183:1299	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	4	57	gly	glycoproteins	678:690	arg1	glycoproteins					678:690	hydroxyproline-rich glycoproteins	658:690	hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells	658:741	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	3	58	located	observed	507:514	arg1	exudate					531:537	the viscous exudate	519:537	the viscous exudate	519:537	In the previous studies, spherical nanoparticles were observed in the viscous exudate.
27217558	3	58	located	observed	507:514	arg1	studies					469:475	the previous studies	456:475	the previous studies	456:475	In the previous studies, spherical nanoparticles were observed in the viscous exudate.
27217558	3	58	located	observed	507:514	arg2	nanoparticles					488:500	spherical nanoparticles	478:500	spherical nanoparticles	478:500	In the previous studies, spherical nanoparticles were observed in the viscous exudate.
27217558	9	59	theme	adhesion	1757:1764	arg1	mechanisms					1766:1775	the adhesion mechanisms	1753:1775	the adhesion mechanisms revealed by English ivy	1753:1799	Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
27217558	8	60	link	ivy-derived	1621:1631	arg1	adhesive					1633:1640	adhesive	1633:1640	adhesive	1633:1640	Information gained from the subsequent tensile tests, in turn, substantiated the proposed adhesion mechanisms underlying the ivy-derived adhesive.
27217558	4	61	theme	extracellular	707:719	arg1	spaces					721:726	the extracellular spaces	703:726	the extracellular spaces of plant cells	703:741	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	5	62	from	ivy	833:835	arg1	surface					892:898	the surface	888:898	the surface of substrates	888:912	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	5	63	theme	substrates	903:912	arg1	surface					892:898	the surface	888:898	the surface of substrates	888:912	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	7	64	theme	reconstructed	1340:1352	arg1	composite					1375:1383	a reconstructed ivy-mimetic adhesive composite	1338:1383	a reconstructed ivy-mimetic adhesive composite	1338:1383	Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions.
27217558	5	65	theme	wetting	868:874	arg1	behavior					876:883	the ivy adhesive, and thus a favorable wetting behavior	829:883	behavior	876:883	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	6	66	theme	pectic	1010:1015	arg1	acids					1017:1021	the pectic acids	1006:1021	the pectic acids	1006:1021	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	9	67	theme	English	1789:1795	arg1	ivy					1797:1799	English ivy	1789:1799	English ivy	1789:1799	Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
27217558	4	68	theme	glycoproteins	678:690	arg1	superfamily					643:653	a superfamily	641:653	a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells	641:741	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	4	68	theme	glycoproteins	678:690	arg1	proteins					624:631	arabinogalactan proteins	608:631	arabinogalactan proteins (AGPs)	608:638	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	1	69	theme	climb	304:308	arg1	surfaces					319:326	climb vertical surfaces	304:326	climb vertical surfaces	304:326	Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces.
27217558	1	70	theme	Charles	143:149	arg1	Darwin					151:156	Charles Darwin	143:156	Charles Darwin	143:156	Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces.
27217558	8	71	theme	tensile	1535:1541	arg1	tests					1543:1547	the subsequent tensile tests	1520:1547	the subsequent tensile tests	1520:1547	Information gained from the subsequent tensile tests, in turn, substantiated the proposed adhesion mechanisms underlying the ivy-derived adhesive.
27217558	0	72	theme	English	101:107	arg1	ivy					109:111	English ivy	101:111	English ivy	101:111	Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy.
27217558	5	73	from	behavior	876:883	arg1	surface					892:898	the surface	888:898	the surface of substrates	888:912	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	6	74	theme	adhesive	1068:1075	arg1	substances					1077:1086	the exuded adhesive substances	1057:1086	the exuded adhesive substances	1057:1086	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	0	75	theme	arabinogalactan	14:28	arg1	proteins					30:37	Nanospherical arabinogalactan proteins	0:37	Nanospherical arabinogalactan proteins	0:37	Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy.
27217558	0	75	theme	arabinogalactan	14:28	arg1	component					49:57	a key component	43:57	a key component of the high-strength adhesive secreted by English ivy	43:111	Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy.
27217558	4	76	theme	cells	737:741	arg1	spaces					721:726	the extracellular spaces	703:726	the extracellular spaces of plant cells	703:741	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	5	77	theme	low	812:814	arg1	viscosity					816:824	a low viscosity	810:824	a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates	810:912	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	8	78	theme	proposed	1577:1584	arg1	mechanisms					1595:1604	the proposed adhesion mechanisms	1573:1604	the proposed adhesion mechanisms underlying the ivy-derived adhesive	1573:1640	Information gained from the subsequent tensile tests, in turn, substantiated the proposed adhesion mechanisms underlying the ivy-derived adhesive.
27217558	5	79	theme	ivy	781:783	arg1	nanoparticles					785:797	the AGP-rich ivy nanoparticles	768:797	the AGP-rich ivy nanoparticles	768:797	The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates.
27217558	9	80	theme	diverse	1881:1887	arg1	adhesives					1897:1905	diverse botanic adhesives	1881:1905	diverse botanic adhesives	1881:1905	Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
27217558	2	81	theme	adhesive	443:450	arg1	high-strength					429:441	this high-strength adhesive	424:450	this high-strength adhesive	424:450	Unfortunately, little progress has been made in elucidating the adhesion mechanisms underlying this high-strength adhesive.
27217558	3	82	theme	spherical	478:486	arg1	nanoparticles					488:500	spherical nanoparticles	478:500	spherical nanoparticles	478:500	In the previous studies, spherical nanoparticles were observed in the viscous exudate.
27217558	1	83	theme	plant	295:299	arg1	capacity					278:285	the capacity	274:285	the capacity of this plant to climb vertical surfaces	274:326	Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces.
27217558	4	84	from	spaces	721:726	arg1	present					692:698	present	692:698	present	692:698	Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells.
27217558	6	85	theme	subsequent	1095:1104	arg1	hardening					1114:1122	hardening	1114:1122	hardening	1114:1122	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	6	85	theme	subsequent	1095:1104	arg1	curing					1106:1111	subsequent curing	1095:1111	subsequent curing (hardening)	1095:1123	Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates.
27217558	7	86	theme	molecular	1320:1328	arg1	events					1330:1335	these molecular events	1314:1335	these molecular events	1314:1335	Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions.
28655659	9	0	theme	sensory	1367:1373	arg1	attributes					1387:1396	sensory and storage attributes	1367:1396	sensory and storage attributes of preserved fish	1367:1414	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
28655659	8	1	theme	Ch	1145:1146	arg1	action					1135:1140	the antibacterial action	1117:1140	the antibacterial action of Ch against L. monocytogenes	1117:1171	Scanning micrographs elucidated the antibacterial action of Ch against L. monocytogenes.
28655659	0	2	from	chitosan	7:14	arg1	catfish					97:103	minced catfish	90:103	minced catfish	90:103	Fungal chitosan and Lycium barbarum extract as anti-Listeria and quality preservatives in minced catfish.
28655659	5	3	theme	fish	650:653	arg1	mince					655:659	fish mince	650:659	fish mince	650:659	Chitosan could utterly control L. monocytogenes survival in fish mince and its efficacy was strengthened with added LBE at 0.2 and 0.4%.
28655659	7	4	theme	minces	944:949	arg1	assessment					922:931	The sensorial assessment	908:931	The sensorial assessment of treated minces	908:949	The sensorial assessment of treated minces indicated panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation.
28655659	9	5	theme	fungal	1213:1218	arg1	composites					1227:1236	fungal Ch/LBE composites	1213:1236	fungal Ch/LBE composites	1213:1236	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
28655659	6	6	theme	increased	881:889	arg1	addition					891:898	increased addition	881:898	increased addition of LBE	881:905	Blending of fish mince with Ch could effectively reduce the progress of chemical spoilage parameters and this protective effect was greatly enhanced with increased addition of LBE.
28655659	0	7	theme	minced	90:95	arg1	catfish					97:103	minced catfish	90:103	minced catfish	90:103	Fungal chitosan and Lycium barbarum extract as anti-Listeria and quality preservatives in minced catfish.
28655659	7	8	theme	blended	1012:1018	arg1	samples					1020:1026	blended samples	1012:1026	blended samples	1012:1026	The sensorial assessment of treated minces indicated panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation.
28655659	2	9	theme	foodstuffs	272:281	arg1	biopreservation					283:297	foodstuffs biopreservation	272:297	foodstuffs biopreservation	272:297	Chitosan (Ch) is a bioactive polymer that could be effectively applied for foodstuffs biopreservation.
28655659	3	10	theme	ethnopharmaceutical	332:350	arg1	fruit					352:356	ethnopharmaceutical fruit	332:356	ethnopharmaceutical fruit that have diverse health protecting benefits	332:401	Lycium barbarum (Goji berry) is ethnopharmaceutical fruit that have diverse health protecting benefits.
28655659	7	11	theme	treated	936:942	arg1	minces					944:949	treated minces	936:949	treated minces	936:949	The sensorial assessment of treated minces indicated panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation.
28655659	7	12	with	samples	1020:1026	arg1	Ch					1033:1034	Ch	1033:1034	Ch	1033:1034	The sensorial assessment of treated minces indicated panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation.
28655659	7	12	with	samples	1020:1026	arg1	prolongation					1071:1082	storage prolongation	1063:1082	storage prolongation	1063:1082	The sensorial assessment of treated minces indicated panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation.
28655659	7	12	with	samples	1020:1026	arg1	LBE					1040:1042	LBE	1040:1042	LBE	1040:1042	The sensorial assessment of treated minces indicated panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation.
28655659	9	13	theme	bacterial	1341:1349	arg1	growth					1351:1356	bacterial growth	1341:1356	bacterial growth	1341:1356	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
28655659	1	14	theme	serious	175:181	arg1	risks					190:194	serious health risks	175:194	serious health risks	175:194	Listeria monocytogenes is a foodborne bacterial pathogen that causes serious health risks.
28655659	0	15	from	extract	36:42	arg1	catfish					97:103	minced catfish	90:103	minced catfish	90:103	Fungal chitosan and Lycium barbarum extract as anti-Listeria and quality preservatives in minced catfish.
28655659	5	16	theme	L.	621:622	arg1	survival					638:645	L. monocytogenes survival	621:645	L. monocytogenes survival in fish mince	621:659	Chitosan could utterly control L. monocytogenes survival in fish mince and its efficacy was strengthened with added LBE at 0.2 and 0.4%.
28655659	7	17	theme	sensorial	912:920	arg1	assessment					922:931	The sensorial assessment	908:931	The sensorial assessment of treated minces	908:949	The sensorial assessment of treated minces indicated panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation.
28655659	1	18	theme	health	183:188	arg1	risks					190:194	serious health risks	175:194	serious health risks	175:194	Listeria monocytogenes is a foodborne bacterial pathogen that causes serious health risks.
28655659	3	19	theme	protecting	383:392	arg1	benefits					394:401	diverse health protecting benefits	368:401	diverse health protecting benefits	368:401	Lycium barbarum (Goji berry) is ethnopharmaceutical fruit that have diverse health protecting benefits.
28655659	3	20	theme	diverse	368:374	arg1	benefits					394:401	diverse health protecting benefits	368:401	diverse health protecting benefits	368:401	Lycium barbarum (Goji berry) is ethnopharmaceutical fruit that have diverse health protecting benefits.
28655659	7	21	theme	storage	1063:1069	arg1	prolongation					1071:1082	storage prolongation	1063:1082	storage prolongation	1063:1082	The sensorial assessment of treated minces indicated panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation.
28655659	6	22	with	Blending	727:734	arg1	Ch					755:756	Ch	755:756	Ch	755:756	Blending of fish mince with Ch could effectively reduce the progress of chemical spoilage parameters and this protective effect was greatly enhanced with increased addition of LBE.
28655659	0	23	theme	Fungal	0:5	arg1	chitosan					7:14	Fungal chitosan	0:14	Fungal chitosan	0:14	Fungal chitosan and Lycium barbarum extract as anti-Listeria and quality preservatives in minced catfish.
28655659	4	24	theme	African	546:552	arg1	catfish					554:560	African catfish	546:560	African catfish mince (Clarias gariepinus)	546:587	Chitosan was produced from A. niger and employed with L. barbarum extract (LBE) as blends for Listeria control and quality biopreservation of African catfish mince (Clarias gariepinus).
28655659	9	25	theme	composites	1227:1236	arg1	application					1198:1208	the application	1194:1208	the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents	1194:1282	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
28655659	6	26	theme	protective	837:846	arg1	effect					848:853	this protective effect	832:853	this protective effect	832:853	Blending of fish mince with Ch could effectively reduce the progress of chemical spoilage parameters and this protective effect was greatly enhanced with increased addition of LBE.
28655659	0	27	theme	Lycium	20:25	arg1	extract					36:42	Lycium barbarum extract	20:42	Lycium barbarum extract	20:42	Fungal chitosan and Lycium barbarum extract as anti-Listeria and quality preservatives in minced catfish.
28655659	5	28	from	survival	638:645	arg1	mince					655:659	fish mince	650:659	fish mince	650:659	Chitosan could utterly control L. monocytogenes survival in fish mince and its efficacy was strengthened with added LBE at 0.2 and 0.4%.
28655659	6	29	theme	mince	744:748	arg1	Blending					727:734	Blending	727:734	Blending of fish mince with Ch	727:756	Blending of fish mince with Ch could effectively reduce the progress of chemical spoilage parameters and this protective effect was greatly enhanced with increased addition of LBE.
28655659	3	30	theme	health	376:381	arg1	benefits					394:401	diverse health protecting benefits	368:401	diverse health protecting benefits	368:401	Lycium barbarum (Goji berry) is ethnopharmaceutical fruit that have diverse health protecting benefits.
28655659	4	31	theme	mince	562:566	arg1	control					507:513	Listeria control	498:513	Listeria control	498:513	Chitosan was produced from A. niger and employed with L. barbarum extract (LBE) as blends for Listeria control and quality biopreservation of African catfish mince (Clarias gariepinus).
28655659	4	31	theme	mince	562:566	arg1	biopreservation					527:541	quality biopreservation	519:541	quality biopreservation	519:541	Chitosan was produced from A. niger and employed with L. barbarum extract (LBE) as blends for Listeria control and quality biopreservation of African catfish mince (Clarias gariepinus).
28655659	5	32	theme	added	700:704	arg1	LBE					706:708	added LBE	700:708	added LBE at 0.2 and 0.4%	700:724	Chitosan could utterly control L. monocytogenes survival in fish mince and its efficacy was strengthened with added LBE at 0.2 and 0.4%.
28655659	1	33	theme	Listeria	106:113	arg1	pathogen					154:161	a foodborne bacterial pathogen	132:161	a foodborne bacterial pathogen that causes serious health risks	132:194	Listeria monocytogenes is a foodborne bacterial pathogen that causes serious health risks.
28655659	1	33	theme	Listeria	106:113	arg1	monocytogenes					115:127	Listeria monocytogenes	106:127	Listeria monocytogenes	106:127	Listeria monocytogenes is a foodborne bacterial pathogen that causes serious health risks.
28655659	4	34	theme	catfish	554:560	arg1	mince					562:566	African catfish mince	546:566	African catfish mince (Clarias gariepinus)	546:587	Chitosan was produced from A. niger and employed with L. barbarum extract (LBE) as blends for Listeria control and quality biopreservation of African catfish mince (Clarias gariepinus).
28655659	8	35	theme	Scanning	1085:1092	arg1	micrographs					1094:1104	Scanning micrographs	1085:1104	Scanning micrographs	1085:1104	Scanning micrographs elucidated the antibacterial action of Ch against L. monocytogenes.
28655659	5	36	from	%	724:724	arg1	LBE					706:708	added LBE	700:708	added LBE at 0.2 and 0.4%	700:724	Chitosan could utterly control L. monocytogenes survival in fish mince and its efficacy was strengthened with added LBE at 0.2 and 0.4%.
28655659	9	37	theme	catfish	1313:1319	arg1	mince					1321:1325	catfish mince	1313:1325	catfish mince	1313:1325	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
28655659	0	38	theme	barbarum	27:34	arg1	extract					36:42	Lycium barbarum extract	20:42	Lycium barbarum extract	20:42	Fungal chitosan and Lycium barbarum extract as anti-Listeria and quality preservatives in minced catfish.
28655659	9	39	theme	storage	1379:1385	arg1	attributes					1387:1396	sensory and storage attributes	1367:1396	sensory and storage attributes of preserved fish	1367:1414	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
28655659	4	40	theme	barbarum	461:468	arg1	LBE					479:481	LBE	479:481	LBE	479:481	Chitosan was produced from A. niger and employed with L. barbarum extract (LBE) as blends for Listeria control and quality biopreservation of African catfish mince (Clarias gariepinus).
28655659	4	40	theme	barbarum	461:468	arg1	extract					470:476	L. barbarum extract	458:476	L. barbarum extract (LBE)	458:482	Chitosan was produced from A. niger and employed with L. barbarum extract (LBE) as blends for Listeria control and quality biopreservation of African catfish mince (Clarias gariepinus).
28655659	2	41	theme	bioactive	216:224	arg1	polymer					226:232	a bioactive polymer	214:232	a bioactive polymer that could be effectively applied for foodstuffs biopreservation	214:297	Chitosan (Ch) is a bioactive polymer that could be effectively applied for foodstuffs biopreservation.
28655659	2	41	theme	bioactive	216:224	arg1	Chitosan					197:204	Chitosan	197:204	Chitosan (Ch)	197:209	Chitosan (Ch) is a bioactive polymer that could be effectively applied for foodstuffs biopreservation.
28655659	4	42	theme	Listeria	498:505	arg1	control					507:513	Listeria control	498:513	Listeria control	498:513	Chitosan was produced from A. niger and employed with L. barbarum extract (LBE) as blends for Listeria control and quality biopreservation of African catfish mince (Clarias gariepinus).
28655659	9	43	theme	preserved	1401:1409	arg1	fish					1411:1414	preserved fish	1401:1414	preserved fish	1401:1414	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
28655659	0	44	theme	anti-Listeria	47:59	arg1	preservatives					73:85	anti-Listeria and quality preservatives	47:85	anti-Listeria and quality preservatives	47:85	Fungal chitosan and Lycium barbarum extract as anti-Listeria and quality preservatives in minced catfish.
28655659	4	45	theme	L.	458:459	arg1	LBE					479:481	LBE	479:481	LBE	479:481	Chitosan was produced from A. niger and employed with L. barbarum extract (LBE) as blends for Listeria control and quality biopreservation of African catfish mince (Clarias gariepinus).
28655659	4	45	theme	L.	458:459	arg1	extract					470:476	L. barbarum extract	458:476	L. barbarum extract (LBE)	458:482	Chitosan was produced from A. niger and employed with L. barbarum extract (LBE) as blends for Listeria control and quality biopreservation of African catfish mince (Clarias gariepinus).
28655659	7	46	theme	entire	991:996	arg1	attributes					998:1007	the entire attributes	987:1007	the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation	987:1082	The sensorial assessment of treated minces indicated panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation.
28655659	5	47	dep	L.	621:622	arg1	monocytogenes					624:636	monocytogenes	624:636	monocytogenes	624:636	Chitosan could utterly control L. monocytogenes survival in fish mince and its efficacy was strengthened with added LBE at 0.2 and 0.4%.
28655659	6	48	theme	parameters	817:826	arg1	progress					787:794	the progress	783:794	the progress of chemical spoilage parameters	783:826	Blending of fish mince with Ch could effectively reduce the progress of chemical spoilage parameters and this protective effect was greatly enhanced with increased addition of LBE.
28655659	3	49	contain	have	363:366	arg1	fruit					352:356	ethnopharmaceutical fruit	332:356	ethnopharmaceutical fruit that have diverse health protecting benefits	332:401	Lycium barbarum (Goji berry) is ethnopharmaceutical fruit that have diverse health protecting benefits.
28655659	3	49	contain	have	363:366	arg2	benefits					394:401	diverse health protecting benefits	368:401	diverse health protecting benefits	368:401	Lycium barbarum (Goji berry) is ethnopharmaceutical fruit that have diverse health protecting benefits.
28655659	9	50	theme	anti-listerial	1262:1275	arg1	agents					1277:1282	anti-listerial agents	1262:1282	anti-listerial agents	1262:1282	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
28655659	4	51	theme	quality	519:525	arg1	biopreservation					527:541	quality biopreservation	519:541	quality biopreservation	519:541	Chitosan was produced from A. niger and employed with L. barbarum extract (LBE) as blends for Listeria control and quality biopreservation of African catfish mince (Clarias gariepinus).
28655659	7	52	theme	samples	1020:1026	arg1	attributes					998:1007	the entire attributes	987:1007	the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation	987:1082	The sensorial assessment of treated minces indicated panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation.
28655659	6	53	theme	spoilage	808:815	arg1	parameters					817:826	chemical spoilage parameters	799:826	chemical spoilage parameters	799:826	Blending of fish mince with Ch could effectively reduce the progress of chemical spoilage parameters and this protective effect was greatly enhanced with increased addition of LBE.
28655659	9	54	theme	fish	1411:1414	arg1	attributes					1387:1396	sensory and storage attributes	1367:1396	sensory and storage attributes of preserved fish	1367:1414	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
28655659	3	55	theme	Lycium	300:305	arg1	barbarum					307:314	Lycium barbarum	300:314	Lycium barbarum (Goji berry)	300:327	Lycium barbarum (Goji berry) is ethnopharmaceutical fruit that have diverse health protecting benefits.
28655659	3	55	theme	Lycium	300:305	arg1	berry					322:326	berry	322:326	berry	322:326	Lycium barbarum (Goji berry) is ethnopharmaceutical fruit that have diverse health protecting benefits.
28655659	6	56	theme	chemical	799:806	arg1	parameters					817:826	chemical spoilage parameters	799:826	chemical spoilage parameters	799:826	Blending of fish mince with Ch could effectively reduce the progress of chemical spoilage parameters and this protective effect was greatly enhanced with increased addition of LBE.
28655659	6	57	theme	fish	739:742	arg1	mince					744:748	fish mince	739:748	fish mince	739:748	Blending of fish mince with Ch could effectively reduce the progress of chemical spoilage parameters and this protective effect was greatly enhanced with increased addition of LBE.
28655659	8	58	theme	antibacterial	1121:1133	arg1	action					1135:1140	the antibacterial action	1117:1140	the antibacterial action of Ch against L. monocytogenes	1117:1171	Scanning micrographs elucidated the antibacterial action of Ch against L. monocytogenes.
28655659	1	59	theme	foodborne	134:142	arg1	pathogen					154:161	a foodborne bacterial pathogen	132:161	a foodborne bacterial pathogen that causes serious health risks	132:194	Listeria monocytogenes is a foodborne bacterial pathogen that causes serious health risks.
28655659	1	59	theme	foodborne	134:142	arg1	monocytogenes					115:127	Listeria monocytogenes	106:127	Listeria monocytogenes	106:127	Listeria monocytogenes is a foodborne bacterial pathogen that causes serious health risks.
28655659	0	60	theme	quality	65:71	arg1	preservatives					73:85	anti-Listeria and quality preservatives	47:85	anti-Listeria and quality preservatives	47:85	Fungal chitosan and Lycium barbarum extract as anti-Listeria and quality preservatives in minced catfish.
28655659	1	61	theme	bacterial	144:152	arg1	pathogen					154:161	a foodborne bacterial pathogen	132:161	a foodborne bacterial pathogen that causes serious health risks	132:194	Listeria monocytogenes is a foodborne bacterial pathogen that causes serious health risks.
28655659	1	61	theme	bacterial	144:152	arg1	monocytogenes					115:127	Listeria monocytogenes	106:127	Listeria monocytogenes	106:127	Listeria monocytogenes is a foodborne bacterial pathogen that causes serious health risks.
28655659	9	62	theme	Ch/LBE	1220:1225	arg1	composites					1227:1236	fungal Ch/LBE composites	1213:1236	fungal Ch/LBE composites	1213:1236	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
28655659	9	63	with	blending	1299:1306	arg1	mince					1321:1325	catfish mince	1313:1325	catfish mince	1313:1325	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
28655659	6	64	theme	LBE	903:905	arg1	addition					891:898	increased addition	881:898	increased addition of LBE	881:905	Blending of fish mince with Ch could effectively reduce the progress of chemical spoilage parameters and this protective effect was greatly enhanced with increased addition of LBE.
28655659	7	65	theme	panelists	961:969	arg1	preferences					971:981	panelists preferences	961:981	panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation	961:1082	The sensorial assessment of treated minces indicated panelists preferences for the entire attributes of blended samples with Ch and LBE, particularly with storage prolongation.
28655659	9	66	dep	eliminate	1331:1339	arg1	enhance					1359:1365	enhance	1359:1365	enhance sensory and storage attributes of preserved fish	1359:1414	Results recommended the application of fungal Ch/LBE composites as biopreservatives and anti-listerial agents, through their blending with catfish mince, to eliminate bacterial growth, enhance sensory and storage attributes of preserved fish.
26295368	5	0	from	embedded	988:995	arg1	basophils					1000:1008	basophils	1000:1008	basophils	1000:1008	However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments.
26295368	5	1	theme	nonaqueous	1228:1237	arg1	environments					1239:1250	nonaqueous environments	1228:1250	nonaqueous environments	1228:1250	However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments.
26295368	4	2	theme	dipolar	779:785	arg1	arrangements					787:798	parallel dipolar arrangements	770:798	parallel dipolar arrangements	770:798	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	5	3	from	nm	1113:1114	arg1	peaks					1091:1095	two peaks	1087:1095	two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments	1087:1250	However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments.
26295368	2	4	theme	variables	256:264	arg1	influence					243:251	influence	243:251	influence of variables such as pH, viscosity, salt composition, and various proteins on the absorption spectrum of BO21	243:361	Results obtained with solutions indicate that influence of variables such as pH, viscosity, salt composition, and various proteins on the absorption spectrum of BO21 is negligible.
26295368	5	5	theme	intracellular	1037:1049	arg1	BO21					1051:1054	intracellular BO21	1037:1054	intracellular BO21	1037:1054	However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments.
26295368	4	6	theme	parallel	770:777	arg1	arrangements					787:798	parallel dipolar arrangements	770:798	parallel dipolar arrangements	770:798	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	7	from	solutions	583:591	arg1	favorable					650:658	favorable	650:658	favorable	650:658	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	7	from	solutions	583:591	arg1	a					594:594	a	594:594	a	594:594	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	7	from	solutions	583:591	arg1	formation					601:609	the formation	597:609	the formation of BO21 oligomers	597:627	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	5	8	theme	BO21	1051:1054	arg1	spectra					1026:1032	the absorption spectra	1011:1032	the absorption spectra of intracellular BO21	1011:1054	However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments.
26295368	5	8	theme	BO21	1051:1054	arg1	red-shifted					1069:1079	red-shifted	1069:1079	red-shifted	1069:1079	However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments.
26295368	4	9	dep	association	805:815	arg1	b					802:802	b	802:802	b	802:802	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	3	10	theme	blue	417:420	arg1	shift					422:426	a blue shift	415:426	a blue shift (484-465 nm)	415:439	However, in the presence of heparin, a blue shift (484-465 nm) is observed, which is attributed to the aggregation of BO21 on the polyanion.
26295368	3	10	theme	blue	417:420	arg1	nm					437:438	484-465 nm	429:438	484-465 nm	429:438	However, in the presence of heparin, a blue shift (484-465 nm) is observed, which is attributed to the aggregation of BO21 on the polyanion.
26295368	4	11	dep	due	900:902	arg1	shift					891:895	the blue shift	882:895	the blue shift	882:895	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	11	dep	due	900:902	arg1	c					879:879	c	879:879	c	879:879	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	11	dep	due	900:902	arg1	due					900:902	due	900:902	due	900:902	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	12	theme	molecule	955:962	arg1	stacking					964:971	BO21 molecule stacking	950:971	BO21 molecule stacking	950:971	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	13	dep	favorable	650:658	arg1	favorable					650:658	favorable	650:658	favorable	650:658	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	13	dep	favorable	650:658	arg1	a					594:594	a	594:594	a	594:594	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	13	dep	favorable	650:658	arg1	formation					601:609	the formation	597:609	the formation of BO21 oligomers	597:627	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	2	14	theme	absorption	335:344	arg1	spectrum					346:353	the absorption spectrum	331:353	the absorption spectrum of BO21	331:361	Results obtained with solutions indicate that influence of variables such as pH, viscosity, salt composition, and various proteins on the absorption spectrum of BO21 is negligible.
26295368	6	15	theme	Initial	1253:1259	arg1	evidence					1261:1268	Initial evidence	1253:1268	Initial evidence of the ability to differentiate between leukocyte types by BO21	1253:1332	Initial evidence of the ability to differentiate between leukocyte types by BO21 is presented.
26295368	5	16	theme	absorption	1015:1024	arg1	spectra					1026:1032	the absorption spectra	1011:1032	the absorption spectra of intracellular BO21	1011:1054	However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments.
26295368	5	16	theme	absorption	1015:1024	arg1	red-shifted					1069:1079	red-shifted	1069:1079	red-shifted	1069:1079	However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments.
26295368	5	17	from	basophils	1000:1008	arg1	embedded					988:995	embedded	988:995	embedded	988:995	However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments.
26295368	4	18	from	favorable	650:658	arg1	solutions					583:591	aqueous solutions	575:591	aqueous solutions	575:591	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	1	19	theme	Spectroscopic	71:83	arg1	properties					85:94	Spectroscopic properties	71:94	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes	71:180	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes were examined.
26295368	4	20	theme	blue	886:889	arg1	shift					891:895	the blue shift	882:895	the blue shift	882:895	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	20	theme	blue	886:889	arg1	c					879:879	c	879:879	c	879:879	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	20	theme	blue	886:889	arg1	due					900:902	due	900:902	due	900:902	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	3	21	from	aggregation	481:491	arg1	polyanion					508:516	the polyanion	504:516	the polyanion	504:516	However, in the presence of heparin, a blue shift (484-465 nm) is observed, which is attributed to the aggregation of BO21 on the polyanion.
26295368	4	22	theme	aqueous	575:581	arg1	solutions					583:591	aqueous solutions	575:591	aqueous solutions	575:591	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	0	23	theme	Spectroscopic	16:28	arg1	Aspects					30:36	the Spectroscopic Aspects	12:36	the Spectroscopic Aspects of Cationic Dye Basic Orange 21	12:68	Analysis of the Spectroscopic Aspects of Cationic Dye Basic Orange 21.
26295368	4	24	theme	transitions	928:938	arg1	mixing					911:916	the mixing	907:916	the mixing of π → π* transitions caused by BO21 molecule stacking	907:971	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	25	theme	functional	536:545	arg1	theory					547:552	density functional theory	528:552	density functional theory	528:552	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	1	26	theme	cationic	99:106	arg1	orange					118:123	cationic dye basic orange 21	99:126	cationic dye basic orange 21 (BO21)	99:133	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes were examined.
26295368	1	26	theme	cationic	99:106	arg1	BO21					129:132	BO21	129:132	BO21	129:132	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes were examined.
26295368	4	27	theme	BO21	950:953	arg1	stacking					964:971	BO21 molecule stacking	950:971	BO21 molecule stacking	950:971	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	28	theme	density	528:534	arg1	theory					547:552	density functional theory	528:552	density functional theory	528:552	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	2	29	theme	salt	289:292	arg1	composition					294:304	salt composition	289:304	salt composition	289:304	Results obtained with solutions indicate that influence of variables such as pH, viscosity, salt composition, and various proteins on the absorption spectrum of BO21 is negligible.
26295368	4	30	theme	BO21	825:828	arg1	aggregates					830:839	BO21 aggregates	825:839	BO21 aggregates	825:839	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	3	31	theme	BO21	496:499	arg1	aggregation					481:491	the aggregation	477:491	the aggregation of BO21 on the polyanion	477:516	However, in the presence of heparin, a blue shift (484-465 nm) is observed, which is attributed to the aggregation of BO21 on the polyanion.
26295368	4	32	theme	π	925:925	arg1	transitions					928:938	π → π* transitions	921:938	π → π* transitions caused by BO21 molecule stacking	921:971	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	2	33	theme	BO21	358:361	arg1	spectrum					346:353	the absorption spectrum	331:353	the absorption spectrum of BO21	331:361	Results obtained with solutions indicate that influence of variables such as pH, viscosity, salt composition, and various proteins on the absorption spectrum of BO21 is negligible.
26295368	0	34	theme	Aspects	30:36	arg1	Analysis					0:7	Analysis	0:7	Analysis of the Spectroscopic Aspects of Cationic Dye Basic Orange 21.	0:69	Analysis of the Spectroscopic Aspects of Cationic Dye Basic Orange 21.
26295368	0	35	theme	Dye	50:52	arg1	Orange					60:65	Cationic Dye Basic Orange 21	41:68	Cationic Dye Basic Orange 21	41:68	Analysis of the Spectroscopic Aspects of Cationic Dye Basic Orange 21.
26295368	6	36	theme	leukocyte	1310:1318	arg1	types					1320:1324	leukocyte types	1310:1324	leukocyte types	1310:1324	Initial evidence of the ability to differentiate between leukocyte types by BO21 is presented.
26295368	4	37	theme	binding	729:735	arg1	energies					737:744	their binding energies	723:744	their binding energies	723:744	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	1	38	theme	dye	108:110	arg1	orange					118:123	cationic dye basic orange 21	99:126	cationic dye basic orange 21 (BO21)	99:133	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes were examined.
26295368	1	38	theme	dye	108:110	arg1	BO21					129:132	BO21	129:132	BO21	129:132	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes were examined.
26295368	0	39	theme	Cationic	41:48	arg1	Orange					60:65	Cationic Dye Basic Orange 21	41:68	Cationic Dye Basic Orange 21	41:68	Analysis of the Spectroscopic Aspects of Cationic Dye Basic Orange 21.
26295368	1	40	theme	basic	112:116	arg1	orange					118:123	cationic dye basic orange 21	99:126	cationic dye basic orange 21 (BO21)	99:133	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes were examined.
26295368	1	40	theme	basic	112:116	arg1	BO21					129:132	BO21	129:132	BO21	129:132	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes were examined.
26295368	2	41	from	influence	243:251	arg1	spectrum					346:353	the absorption spectrum	331:353	the absorption spectrum of BO21	331:361	Results obtained with solutions indicate that influence of variables such as pH, viscosity, salt composition, and various proteins on the absorption spectrum of BO21 is negligible.
26295368	4	42	theme	dipolar	698:704	arg1	arrangement					706:716	an antiparallel dipolar arrangement	682:716	an antiparallel dipolar arrangement	682:716	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	43	theme	antiparallel	685:696	arg1	arrangement					706:716	an antiparallel dipolar arrangement	682:716	an antiparallel dipolar arrangement	682:716	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	1	44	theme	orange	118:123	arg1	properties					85:94	Spectroscopic properties	71:94	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes	71:180	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes were examined.
26295368	4	45	theme	→	923:923	arg1	transitions					928:938	π → π* transitions	921:938	π → π* transitions caused by BO21 molecule stacking	921:971	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	5	46	with	red-shifted	1069:1079	arg1	peaks					1091:1095	two peaks	1087:1095	two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments	1087:1250	However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments.
26295368	4	47	theme	BO21	614:617	arg1	oligomers					619:627	BO21 oligomers	614:627	BO21 oligomers	614:627	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	48	theme	*	926:926	arg1	transitions					928:938	π → π* transitions	921:938	π → π* transitions caused by BO21 molecule stacking	921:971	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	49	theme	oligomers	619:627	arg1	favorable					650:658	favorable	650:658	favorable	650:658	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	49	theme	oligomers	619:627	arg1	a					594:594	a	594:594	a	594:594	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	4	49	theme	oligomers	619:627	arg1	formation					601:609	the formation	597:609	the formation of BO21 oligomers	597:627	Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of π → π* transitions caused by BO21 molecule stacking.
26295368	0	50	theme	Orange	60:65	arg1	Aspects					30:36	the Spectroscopic Aspects	12:36	the Spectroscopic Aspects of Cationic Dye Basic Orange 21	12:68	Analysis of the Spectroscopic Aspects of Cationic Dye Basic Orange 21.
26295368	1	51	from	properties	85:94	arg1	solutions					138:146	solutions	138:146	solutions	138:146	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes were examined.
26295368	1	51	from	properties	85:94	arg1	solids					152:157	solids	152:157	solids	152:157	Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes were examined.
26295368	0	52	theme	Basic	54:58	arg1	Orange					60:65	Cationic Dye Basic Orange 21	41:68	Cationic Dye Basic Orange 21	41:68	Analysis of the Spectroscopic Aspects of Cationic Dye Basic Orange 21.
26295368	3	53	dep	heparin	406:412	arg1	presence					394:401	presence	394:401	presence	394:401	However, in the presence of heparin, a blue shift (484-465 nm) is observed, which is attributed to the aggregation of BO21 on the polyanion.
26295368	3	53	dep	heparin	406:412	arg1	the					390:392	the	390:392	the	390:392	However, in the presence of heparin, a blue shift (484-465 nm) is observed, which is attributed to the aggregation of BO21 on the polyanion.
26295368	5	54	theme	BO21-heparin	1156:1167	arg1	complex					1169:1175	the BO21-heparin complex	1152:1175	the BO21-heparin complex	1152:1175	However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21-heparin complex, respectively, which are intracellularly hosted in nonaqueous environments.
26295368	2	55	theme	various	311:317	arg1	proteins					319:326	various proteins	311:326	various proteins	311:326	Results obtained with solutions indicate that influence of variables such as pH, viscosity, salt composition, and various proteins on the absorption spectrum of BO21 is negligible.
26295368	6	56	theme	ability	1277:1283	arg1	evidence					1261:1268	Initial evidence	1253:1268	Initial evidence of the ability to differentiate between leukocyte types by BO21	1253:1332	Initial evidence of the ability to differentiate between leukocyte types by BO21 is presented.
25311120	8	0	theme	protein	1032:1038	arg1	composition					1040:1050	the protein composition	1028:1050	the protein composition of nucleoli at the specified time point	1028:1090	As a result, this method can capture rapid biochemical changes in nucleoli by freezing the cells at a precise time, hence faithfully reflecting the protein composition of nucleoli at the specified time point.
25311120	9	1	from	studies	1136:1142	arg1	nucleolus					1169:1177	the nucleolus	1165:1177	the nucleolus	1165:1177	This protocol will be useful for proteomic studies of dynamic events in the nucleolus and for better understanding of the biology of mammalian cells.
25311120	5	2	theme	new	582:584	arg1	method					619:624	a new and improved nucleolar isolation method	580:624	a new and improved nucleolar isolation method for cultured adherent cells	580:652	In this chapter we describe a new and improved nucleolar isolation method for cultured adherent cells.
25311120	4	3	theme	cells	522:526	arg1	number					512:517	a large number	504:517	a large number of cells and tedious procedures	504:549	In particular, these methods require the use of a large number of cells and tedious procedures.
25311120	9	4	theme	dynamic	1147:1153	arg1	events					1155:1160	dynamic events	1147:1160	dynamic events in the nucleolus	1147:1177	This protocol will be useful for proteomic studies of dynamic events in the nucleolus and for better understanding of the biology of mammalian cells.
25311120	7	5	theme	starting	865:872	arg1	material					874:881	less starting material	860:881	less starting material	860:881	The nucleoli can be obtained within a time as short as 20 min, and the high yield allows the use of less starting material.
25311120	8	6	theme	biochemical	927:937	arg1	changes					939:945	rapid biochemical changes	921:945	rapid biochemical changes in nucleoli	921:957	As a result, this method can capture rapid biochemical changes in nucleoli by freezing the cells at a precise time, hence faithfully reflecting the protein composition of nucleoli at the specified time point.
25311120	8	6	theme	biochemical	927:937	arg1	result					889:894	a result	887:894	a result	887:894	As a result, this method can capture rapid biochemical changes in nucleoli by freezing the cells at a precise time, hence faithfully reflecting the protein composition of nucleoli at the specified time point.
25311120	7	7	theme	short	806:810	arg1	time					798:801	a time	796:801	a time as short as 20 min	796:820	The nucleoli can be obtained within a time as short as 20 min, and the high yield allows the use of less starting material.
25311120	8	8	theme	rapid	921:925	arg1	changes					939:945	rapid biochemical changes	921:945	rapid biochemical changes in nucleoli	921:957	As a result, this method can capture rapid biochemical changes in nucleoli by freezing the cells at a precise time, hence faithfully reflecting the protein composition of nucleoli at the specified time point.
25311120	8	8	theme	rapid	921:925	arg1	result					889:894	a result	887:894	a result	887:894	As a result, this method can capture rapid biochemical changes in nucleoli by freezing the cells at a precise time, hence faithfully reflecting the protein composition of nucleoli at the specified time point.
25311120	5	9	theme	improved	590:597	arg1	method					619:624	a new and improved nucleolar isolation method	580:624	a new and improved nucleolar isolation method for cultured adherent cells	580:652	In this chapter we describe a new and improved nucleolar isolation method for cultured adherent cells.
25311120	7	10	theme	material	874:881	arg1	use					853:855	the use	849:855	the use of less starting material	849:881	The nucleoli can be obtained within a time as short as 20 min, and the high yield allows the use of less starting material.
25311120	2	11	theme	live	256:259	arg1	technology					274:283	live cell imaging technology	256:283	live cell imaging technology	256:283	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	4	12	theme	tedious	532:538	arg1	procedures					540:549	tedious procedures	532:549	tedious procedures	532:549	In particular, these methods require the use of a large number of cells and tedious procedures.
25311120	5	13	theme	nucleolar	599:607	arg1	method					619:624	a new and improved nucleolar isolation method	580:624	a new and improved nucleolar isolation method for cultured adherent cells	580:652	In this chapter we describe a new and improved nucleolar isolation method for cultured adherent cells.
25311120	1	14	theme	organelles	90:99	arg1	organelles					90:99	the first subcellular organelles	68:99	the first subcellular organelles	68:99	The nucleolus was one of the first subcellular organelles to be isolated from the cell.
25311120	1	14	theme	organelles	90:99	arg1	one					61:63	one	61:63	one	61:63	The nucleolus was one of the first subcellular organelles to be isolated from the cell.
25311120	1	14	theme	organelles	90:99	arg1	nucleolus					47:55	The nucleolus	43:55	The nucleolus	43:55	The nucleolus was one of the first subcellular organelles to be isolated from the cell.
25311120	6	15	theme	sucrose	743:749	arg1	cushion					751:757	a sucrose cushion	741:757	a sucrose cushion	741:757	In this method cells are snap-frozen before direct sonication and centrifugation onto a sucrose cushion.
25311120	3	16	theme	current	370:376	arg1	methods					398:404	current nucleolar isolation methods	370:404	current nucleolar isolation methods	370:404	However, the limitations of current nucleolar isolation methods hinder the further exploration of this structure.
25311120	3	17	theme	isolation	388:396	arg1	methods					398:404	current nucleolar isolation methods	370:404	current nucleolar isolation methods	370:404	However, the limitations of current nucleolar isolation methods hinder the further exploration of this structure.
25311120	8	18	theme	nucleoli	1055:1062	arg1	composition					1040:1050	the protein composition	1028:1050	the protein composition of nucleoli at the specified time point	1028:1090	As a result, this method can capture rapid biochemical changes in nucleoli by freezing the cells at a precise time, hence faithfully reflecting the protein composition of nucleoli at the specified time point.
25311120	3	19	theme	methods	398:404	arg1	limitations					355:365	the limitations	351:365	the limitations of current nucleolar isolation methods	351:404	However, the limitations of current nucleolar isolation methods hinder the further exploration of this structure.
25311120	0	20	theme	new	2:4	arg1	method					12:17	A new rapid method	0:17	A new rapid method for isolating nucleoli.	0:41	A new rapid method for isolating nucleoli.
25311120	9	21	theme	mammalian	1226:1234	arg1	cells					1236:1240	mammalian cells	1226:1240	mammalian cells	1226:1240	This protocol will be useful for proteomic studies of dynamic events in the nucleolus and for better understanding of the biology of mammalian cells.
25311120	9	22	theme	better	1187:1192	arg1	understanding					1194:1206	better understanding	1187:1206	better understanding of the biology of mammalian cells	1187:1240	This protocol will be useful for proteomic studies of dynamic events in the nucleolus and for better understanding of the biology of mammalian cells.
25311120	2	23	theme	proteins	332:339	arg1	dynamics					314:321	the dynamics	310:321	the dynamics of these proteins	310:339	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	8	24	from	changes	939:945	arg1	nucleoli					950:957	nucleoli	950:957	nucleoli	950:957	As a result, this method can capture rapid biochemical changes in nucleoli by freezing the cells at a precise time, hence faithfully reflecting the protein composition of nucleoli at the specified time point.
25311120	9	25	theme	events	1155:1160	arg1	studies					1136:1142	proteomic studies	1126:1142	proteomic studies of dynamic events in the nucleolus	1126:1177	This protocol will be useful for proteomic studies of dynamic events in the nucleolus and for better understanding of the biology of mammalian cells.
25311120	2	26	theme	imaging	266:272	arg1	technology					274:283	live cell imaging technology	256:283	live cell imaging technology	256:283	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	3	27	theme	nucleolar	378:386	arg1	methods					398:404	current nucleolar isolation methods	370:404	current nucleolar isolation methods	370:404	However, the limitations of current nucleolar isolation methods hinder the further exploration of this structure.
25311120	9	28	theme	biology	1215:1221	arg1	understanding					1194:1206	better understanding	1187:1206	better understanding of the biology of mammalian cells	1187:1240	This protocol will be useful for proteomic studies of dynamic events in the nucleolus and for better understanding of the biology of mammalian cells.
25311120	5	29	theme	adherent	639:646	arg1	cells					648:652	cultured adherent cells	630:652	cultured adherent cells	630:652	In this chapter we describe a new and improved nucleolar isolation method for cultured adherent cells.
25311120	7	30	theme	less	860:863	arg1	material					874:881	less starting material	860:881	less starting material	860:881	The nucleoli can be obtained within a time as short as 20 min, and the high yield allows the use of less starting material.
25311120	1	31	theme	first	72:76	arg1	organelles					90:99	the first subcellular organelles	68:99	the first subcellular organelles	68:99	The nucleolus was one of the first subcellular organelles to be isolated from the cell.
25311120	2	32	from	thousands	211:219	arg1	organelle					241:249	this organelle	236:249	this organelle	236:249	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	3	33	theme	further	417:423	arg1	exploration					425:435	the further exploration	413:435	the further exploration of this structure	413:453	However, the limitations of current nucleolar isolation methods hinder the further exploration of this structure.
25311120	0	34	theme	rapid	6:10	arg1	method					12:17	A new rapid method	0:17	A new rapid method for isolating nucleoli.	0:41	A new rapid method for isolating nucleoli.
25311120	4	35	theme	number	512:517	arg1	use					497:499	the use	493:499	the use of a large number of cells and tedious procedures	493:549	In particular, these methods require the use of a large number of cells and tedious procedures.
25311120	2	36	theme	modern	145:150	arg1	techniques					162:171	modern proteomic techniques	145:171	modern proteomic techniques	145:171	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	1	37	theme	subcellular	78:88	arg1	organelles					90:99	the first subcellular organelles	68:99	the first subcellular organelles	68:99	The nucleolus was one of the first subcellular organelles to be isolated from the cell.
25311120	9	38	from	nucleolus	1169:1177	arg1	studies					1136:1142	proteomic studies	1126:1142	proteomic studies of dynamic events in the nucleolus	1126:1177	This protocol will be useful for proteomic studies of dynamic events in the nucleolus and for better understanding of the biology of mammalian cells.
25311120	4	39	theme	large	506:510	arg1	number					512:517	a large number	504:517	a large number of cells and tedious procedures	504:549	In particular, these methods require the use of a large number of cells and tedious procedures.
25311120	2	40	from	proteins	224:231	arg1	organelle					241:249	this organelle	236:249	this organelle	236:249	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	9	41	theme	cells	1236:1240	arg1	biology					1215:1221	the biology	1211:1221	the biology of mammalian cells	1211:1240	This protocol will be useful for proteomic studies of dynamic events in the nucleolus and for better understanding of the biology of mammalian cells.
25311120	8	42	theme	specified	1071:1079	arg1	point					1086:1090	the specified time point	1067:1090	the specified time point	1067:1090	As a result, this method can capture rapid biochemical changes in nucleoli by freezing the cells at a precise time, hence faithfully reflecting the protein composition of nucleoli at the specified time point.
25311120	5	43	theme	isolation	609:617	arg1	method					619:624	a new and improved nucleolar isolation method	580:624	a new and improved nucleolar isolation method for cultured adherent cells	580:652	In this chapter we describe a new and improved nucleolar isolation method for cultured adherent cells.
25311120	9	44	theme	proteomic	1126:1134	arg1	studies					1136:1142	proteomic studies	1126:1142	proteomic studies of dynamic events in the nucleolus	1126:1177	This protocol will be useful for proteomic studies of dynamic events in the nucleolus and for better understanding of the biology of mammalian cells.
25311120	6	45	theme	direct	699:704	arg1	sonication					706:715	sonication	706:715	sonication	706:715	In this method cells are snap-frozen before direct sonication and centrifugation onto a sucrose cushion.
25311120	3	46	theme	structure	445:453	arg1	exploration					425:435	the further exploration	413:435	the further exploration of this structure	413:453	However, the limitations of current nucleolar isolation methods hinder the further exploration of this structure.
25311120	8	47	theme	precise	986:992	arg1	time					994:997	a precise time	984:997	a precise time	984:997	As a result, this method can capture rapid biochemical changes in nucleoli by freezing the cells at a precise time, hence faithfully reflecting the protein composition of nucleoli at the specified time point.
25311120	6	48	from	snap-frozen	680:690	arg1	method					663:668	this method	658:668	this method	658:668	In this method cells are snap-frozen before direct sonication and centrifugation onto a sucrose cushion.
25311120	2	49	from	organelle	241:249	arg1	thousands					211:219	thousands	211:219	thousands of proteins in this organelle	211:249	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	8	50	theme	time	1081:1084	arg1	point					1086:1090	the specified time point	1067:1090	the specified time point	1067:1090	As a result, this method can capture rapid biochemical changes in nucleoli by freezing the cells at a precise time, hence faithfully reflecting the protein composition of nucleoli at the specified time point.
25311120	2	51	theme	cell	261:264	arg1	technology					274:283	live cell imaging technology	256:283	live cell imaging technology	256:283	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	5	52	theme	cultured	630:637	arg1	cells					648:652	cultured adherent cells	630:652	cultured adherent cells	630:652	In this chapter we describe a new and improved nucleolar isolation method for cultured adherent cells.
25311120	2	53	theme	techniques	162:171	arg1	advent					135:140	The advent	131:140	The advent of modern proteomic techniques	131:171	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	2	54	theme	proteins	224:231	arg1	thousands					211:219	thousands	211:219	thousands of proteins in this organelle	211:249	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	4	55	theme	procedures	540:549	arg1	number					512:517	a large number	504:517	a large number of cells and tedious procedures	504:549	In particular, these methods require the use of a large number of cells and tedious procedures.
25311120	2	56	theme	proteomic	152:160	arg1	techniques					162:171	modern proteomic techniques	145:171	modern proteomic techniques	145:171	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	6	57	from	method	663:668	arg1	snap-frozen					680:690	snap-frozen	680:690	snap-frozen	680:690	In this method cells are snap-frozen before direct sonication and centrifugation onto a sucrose cushion.
25311120	7	58	theme	high	831:834	arg1	yield					836:840	the high yield	827:840	the high yield	827:840	The nucleoli can be obtained within a time as short as 20 min, and the high yield allows the use of less starting material.
25311120	9	59	from	events	1155:1160	arg1	nucleolus					1169:1177	the nucleolus	1165:1177	the nucleolus	1165:1177	This protocol will be useful for proteomic studies of dynamic events in the nucleolus and for better understanding of the biology of mammalian cells.
25311120	8	60	from	point	1086:1090	arg1	composition					1040:1050	the protein composition	1028:1050	the protein composition of nucleoli at the specified time point	1028:1090	As a result, this method can capture rapid biochemical changes in nucleoli by freezing the cells at a precise time, hence faithfully reflecting the protein composition of nucleoli at the specified time point.
25311120	2	61	theme	thousands	211:219	arg1	identification					193:206	the identification	189:206	the identification of thousands of proteins in this organelle	189:249	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25311120	2	62	theme	dynamics	314:321	arg1	study					301:305	the study	297:305	the study of the dynamics of these proteins	297:339	The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins.
25857965	0	0	theme	antibacterial	81:93	arg1	activity					95:102	intrinsic antibacterial activity	71:102	intrinsic antibacterial activity	71:102	Novel zinc alginate hydrogels prepared by internal setting method with intrinsic antibacterial activity.
25857965	5	1	theme	cytotoxicity	753:764	arg1	level					766:770	the cytotoxicity level	749:770	the cytotoxicity level	749:770	Release of zinc in physiological environments showed that concentration of zinc released in solution lies below the cytotoxicity level.
25857965	3	2	from	stability	371:379	arg1	solution					398:405	normal saline solution	384:405	normal saline solution	384:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	1	3	theme	alginate	145:152	arg1	gelation					133:140	a controlled gelation	120:140	a controlled gelation of alginate	120:152	In this paper, a controlled gelation of alginate was performed for the first time using ZnCO3 and GDL.
25857965	0	4	theme	intrinsic	71:79	arg1	activity					95:102	intrinsic antibacterial activity	71:102	intrinsic antibacterial activity	71:102	Novel zinc alginate hydrogels prepared by internal setting method with intrinsic antibacterial activity.
25857965	4	5	theme	Gelation	508:515	arg1	rate					517:520	Gelation rate	508:520	Gelation rate	508:520	Gelation rate increased at increasing zinc content, while a decrease in water uptake and an improvement of stability were found.
25857965	8	6	theme	antibacterial	1000:1012	arg1	activity					1014:1021	antibacterial activity	1000:1021	antibacterial activity	1000:1021	The resulting hydrogel preserved homogeneity and antibacterial activity.
25857965	3	7	theme	concentration	493:505	arg1	stability					371:379	stability	371:379	stability in normal saline solution	371:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	7	theme	concentration	493:505	arg1	Homogeneity					298:308	Homogeneity	298:308	Homogeneity	298:308	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	7	theme	concentration	493:505	arg1	properties					419:428	mechanical properties	408:428	mechanical properties	408:428	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	7	theme	concentration	493:505	arg1	capability					335:344	swelling capability	326:344	swelling capability	326:344	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	7	theme	concentration	493:505	arg1	function					476:483	a function	474:483	a function of zinc concentration	474:505	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	7	theme	concentration	493:505	arg1	content					317:323	water content	311:323	water content	311:323	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	7	theme	concentration	493:505	arg1	activity					448:455	antibacterial activity	434:455	antibacterial activity	434:455	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	7	theme	concentration	493:505	arg1	rate					365:368	water evaporation rate	347:368	water evaporation rate	347:368	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	9	8	theme	mechanical	1084:1093	arg1	properties					1095:1104	mechanical properties	1084:1104	mechanical properties	1084:1104	Furthermore, it showed even an improvement of stability and mechanical properties, which makes it suitable as long-lasting wound dressing.
25857965	2	9	theme	potential	271:279	arg1	dressings					287:295	potential wound dressings	271:295	potential wound dressings	271:295	Uniform and transparent gels were obtained and investigated as potential wound dressings.
25857965	2	9	theme	potential	271:279	arg1	gels					232:235	Uniform and transparent gels	208:235	Uniform and transparent gels	208:235	Uniform and transparent gels were obtained and investigated as potential wound dressings.
25857965	9	10	theme	properties	1095:1104	arg1	improvement					1055:1065	an improvement	1052:1065	an improvement	1052:1065	Furthermore, it showed even an improvement of stability and mechanical properties, which makes it suitable as long-lasting wound dressing.
25857965	4	11	theme	water	580:584	arg1	uptake					586:591	water uptake	580:591	water uptake	580:591	Gelation rate increased at increasing zinc content, while a decrease in water uptake and an improvement of stability were found.
25857965	3	12	theme	water	347:351	arg1	stability					371:379	stability	371:379	stability in normal saline solution	371:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	12	theme	water	347:351	arg1	Homogeneity					298:308	Homogeneity	298:308	Homogeneity	298:308	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	12	theme	water	347:351	arg1	properties					419:428	mechanical properties	408:428	mechanical properties	408:428	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	12	theme	water	347:351	arg1	capability					335:344	swelling capability	326:344	swelling capability	326:344	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	12	theme	water	347:351	arg1	function					476:483	a function	474:483	a function of zinc concentration	474:505	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	12	theme	water	347:351	arg1	content					317:323	water content	311:323	water content	311:323	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	12	theme	water	347:351	arg1	activity					448:455	antibacterial activity	434:455	antibacterial activity	434:455	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	12	theme	water	347:351	arg1	rate					365:368	water evaporation rate	347:368	water evaporation rate	347:368	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	5	13	theme	zinc	648:651	arg1	Release					637:643	Release	637:643	Release of zinc in physiological environments	637:681	Release of zinc in physiological environments showed that concentration of zinc released in solution lies below the cytotoxicity level.
25857965	3	14	theme	zinc	488:491	arg1	concentration					493:505	zinc concentration	488:505	zinc concentration	488:505	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	15	theme	swelling	326:333	arg1	stability					371:379	stability	371:379	stability in normal saline solution	371:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	15	theme	swelling	326:333	arg1	Homogeneity					298:308	Homogeneity	298:308	Homogeneity	298:308	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	15	theme	swelling	326:333	arg1	properties					419:428	mechanical properties	408:428	mechanical properties	408:428	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	15	theme	swelling	326:333	arg1	capability					335:344	swelling capability	326:344	swelling capability	326:344	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	15	theme	swelling	326:333	arg1	function					476:483	a function	474:483	a function of zinc concentration	474:505	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	15	theme	swelling	326:333	arg1	content					317:323	water content	311:323	water content	311:323	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	15	theme	swelling	326:333	arg1	activity					448:455	antibacterial activity	434:455	antibacterial activity	434:455	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	15	theme	swelling	326:333	arg1	rate					365:368	water evaporation rate	347:368	water evaporation rate	347:368	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	16	from	rate	365:368	arg1	solution					398:405	normal saline solution	384:405	normal saline solution	384:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	0	17	with	method	59:64	arg1	activity					95:102	intrinsic antibacterial activity	71:102	intrinsic antibacterial activity	71:102	Novel zinc alginate hydrogels prepared by internal setting method with intrinsic antibacterial activity.
25857965	3	18	theme	normal	384:389	arg1	solution					398:405	normal saline solution	384:405	normal saline solution	384:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	6	19	theme	antimicrobial	790:802	arg1	activity					804:811	antimicrobial activity	790:811	antimicrobial activity against Escherichia coli	790:836	Hydrogels showed antimicrobial activity against Escherichia coli.
25857965	7	20	theme	highest	857:863	arg1	content					870:876	highest zinc content	857:876	highest zinc content	857:876	The hydrogel with highest zinc content was stabilized with calcium by immersion in a calcium chloride solution.
25857965	4	21	from	decrease	568:575	arg1	uptake					586:591	water uptake	580:591	water uptake	580:591	Gelation rate increased at increasing zinc content, while a decrease in water uptake and an improvement of stability were found.
25857965	0	22	theme	zinc	6:9	arg1	hydrogels					20:28	Novel zinc alginate hydrogels	0:28	Novel zinc alginate hydrogels	0:28	Novel zinc alginate hydrogels prepared by internal setting method with intrinsic antibacterial activity.
25857965	3	23	theme	water	311:315	arg1	stability					371:379	stability	371:379	stability in normal saline solution	371:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	23	theme	water	311:315	arg1	Homogeneity					298:308	Homogeneity	298:308	Homogeneity	298:308	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	23	theme	water	311:315	arg1	properties					419:428	mechanical properties	408:428	mechanical properties	408:428	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	23	theme	water	311:315	arg1	capability					335:344	swelling capability	326:344	swelling capability	326:344	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	23	theme	water	311:315	arg1	function					476:483	a function	474:483	a function of zinc concentration	474:505	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	23	theme	water	311:315	arg1	content					317:323	water content	311:323	water content	311:323	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	23	theme	water	311:315	arg1	activity					448:455	antibacterial activity	434:455	antibacterial activity	434:455	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	23	theme	water	311:315	arg1	rate					365:368	water evaporation rate	347:368	water evaporation rate	347:368	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	7	24	theme	zinc	865:868	arg1	content					870:876	highest zinc content	857:876	highest zinc content	857:876	The hydrogel with highest zinc content was stabilized with calcium by immersion in a calcium chloride solution.
25857965	0	25	theme	Novel	0:4	arg1	hydrogels					20:28	Novel zinc alginate hydrogels	0:28	Novel zinc alginate hydrogels	0:28	Novel zinc alginate hydrogels prepared by internal setting method with intrinsic antibacterial activity.
25857965	7	26	theme	chloride	932:939	arg1	solution					941:948	a calcium chloride solution	922:948	a calcium chloride solution	922:948	The hydrogel with highest zinc content was stabilized with calcium by immersion in a calcium chloride solution.
25857965	9	27	theme	wound	1147:1151	arg1	dressing					1153:1160	long-lasting wound dressing	1134:1160	long-lasting wound dressing	1134:1160	Furthermore, it showed even an improvement of stability and mechanical properties, which makes it suitable as long-lasting wound dressing.
25857965	1	28	theme	first	176:180	arg1	time					182:185	the first time	172:185	the first time using ZnCO3 and GDL	172:205	In this paper, a controlled gelation of alginate was performed for the first time using ZnCO3 and GDL.
25857965	3	29	theme	evaporation	353:363	arg1	stability					371:379	stability	371:379	stability in normal saline solution	371:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	29	theme	evaporation	353:363	arg1	Homogeneity					298:308	Homogeneity	298:308	Homogeneity	298:308	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	29	theme	evaporation	353:363	arg1	properties					419:428	mechanical properties	408:428	mechanical properties	408:428	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	29	theme	evaporation	353:363	arg1	capability					335:344	swelling capability	326:344	swelling capability	326:344	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	29	theme	evaporation	353:363	arg1	function					476:483	a function	474:483	a function of zinc concentration	474:505	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	29	theme	evaporation	353:363	arg1	content					317:323	water content	311:323	water content	311:323	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	29	theme	evaporation	353:363	arg1	activity					448:455	antibacterial activity	434:455	antibacterial activity	434:455	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	29	theme	evaporation	353:363	arg1	rate					365:368	water evaporation rate	347:368	water evaporation rate	347:368	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	4	30	theme	zinc	546:549	arg1	content					551:557	zinc content	546:557	zinc content	546:557	Gelation rate increased at increasing zinc content, while a decrease in water uptake and an improvement of stability were found.
25857965	4	31	theme	stability	615:623	arg1	decrease					568:575	a decrease	566:575	a decrease in water uptake	566:591	Gelation rate increased at increasing zinc content, while a decrease in water uptake and an improvement of stability were found.
25857965	4	31	theme	stability	615:623	arg1	improvement					600:610	an improvement	597:610	an improvement of stability	597:623	Gelation rate increased at increasing zinc content, while a decrease in water uptake and an improvement of stability were found.
25857965	0	32	theme	alginate	11:18	arg1	hydrogels					20:28	Novel zinc alginate hydrogels	0:28	Novel zinc alginate hydrogels	0:28	Novel zinc alginate hydrogels prepared by internal setting method with intrinsic antibacterial activity.
25857965	2	33	theme	wound	281:285	arg1	dressings					287:295	potential wound dressings	271:295	potential wound dressings	271:295	Uniform and transparent gels were obtained and investigated as potential wound dressings.
25857965	2	33	theme	wound	281:285	arg1	gels					232:235	Uniform and transparent gels	208:235	Uniform and transparent gels	208:235	Uniform and transparent gels were obtained and investigated as potential wound dressings.
25857965	9	34	theme	long-lasting	1134:1145	arg1	dressing					1153:1160	long-lasting wound dressing	1134:1160	long-lasting wound dressing	1134:1160	Furthermore, it showed even an improvement of stability and mechanical properties, which makes it suitable as long-lasting wound dressing.
25857965	3	35	theme	saline	391:396	arg1	solution					398:405	normal saline solution	384:405	normal saline solution	384:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	36	from	capability	335:344	arg1	solution					398:405	normal saline solution	384:405	normal saline solution	384:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	37	theme	mechanical	408:417	arg1	stability					371:379	stability	371:379	stability in normal saline solution	371:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	37	theme	mechanical	408:417	arg1	Homogeneity					298:308	Homogeneity	298:308	Homogeneity	298:308	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	37	theme	mechanical	408:417	arg1	properties					419:428	mechanical properties	408:428	mechanical properties	408:428	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	37	theme	mechanical	408:417	arg1	capability					335:344	swelling capability	326:344	swelling capability	326:344	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	37	theme	mechanical	408:417	arg1	function					476:483	a function	474:483	a function of zinc concentration	474:505	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	37	theme	mechanical	408:417	arg1	content					317:323	water content	311:323	water content	311:323	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	37	theme	mechanical	408:417	arg1	activity					448:455	antibacterial activity	434:455	antibacterial activity	434:455	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	37	theme	mechanical	408:417	arg1	rate					365:368	water evaporation rate	347:368	water evaporation rate	347:368	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	38	theme	antibacterial	434:446	arg1	stability					371:379	stability	371:379	stability in normal saline solution	371:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	38	theme	antibacterial	434:446	arg1	Homogeneity					298:308	Homogeneity	298:308	Homogeneity	298:308	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	38	theme	antibacterial	434:446	arg1	properties					419:428	mechanical properties	408:428	mechanical properties	408:428	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	38	theme	antibacterial	434:446	arg1	capability					335:344	swelling capability	326:344	swelling capability	326:344	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	38	theme	antibacterial	434:446	arg1	function					476:483	a function	474:483	a function of zinc concentration	474:505	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	38	theme	antibacterial	434:446	arg1	content					317:323	water content	311:323	water content	311:323	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	38	theme	antibacterial	434:446	arg1	activity					448:455	antibacterial activity	434:455	antibacterial activity	434:455	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	3	38	theme	antibacterial	434:446	arg1	rate					365:368	water evaporation rate	347:368	water evaporation rate	347:368	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	5	39	theme	zinc	712:715	arg1	concentration					695:707	concentration	695:707	concentration of zinc released in solution	695:736	Release of zinc in physiological environments showed that concentration of zinc released in solution lies below the cytotoxicity level.
25857965	7	40	with	hydrogel	843:850	arg1	content					870:876	highest zinc content	857:876	highest zinc content	857:876	The hydrogel with highest zinc content was stabilized with calcium by immersion in a calcium chloride solution.
25857965	2	41	theme	Uniform	208:214	arg1	gels					232:235	Uniform and transparent gels	208:235	Uniform and transparent gels	208:235	Uniform and transparent gels were obtained and investigated as potential wound dressings.
25857965	2	41	theme	Uniform	208:214	arg1	dressings					287:295	potential wound dressings	271:295	potential wound dressings	271:295	Uniform and transparent gels were obtained and investigated as potential wound dressings.
25857965	0	42	theme	setting	51:57	arg1	method					59:64	internal setting method	42:64	internal setting method with intrinsic antibacterial activity	42:102	Novel zinc alginate hydrogels prepared by internal setting method with intrinsic antibacterial activity.
25857965	3	43	from	activity	448:455	arg1	solution					398:405	normal saline solution	384:405	normal saline solution	384:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	0	44	theme	internal	42:49	arg1	method					59:64	internal setting method	42:64	internal setting method with intrinsic antibacterial activity	42:102	Novel zinc alginate hydrogels prepared by internal setting method with intrinsic antibacterial activity.
25857965	5	45	theme	physiological	656:668	arg1	environments					670:681	physiological environments	656:681	physiological environments	656:681	Release of zinc in physiological environments showed that concentration of zinc released in solution lies below the cytotoxicity level.
25857965	5	46	from	Release	637:643	arg1	environments					670:681	physiological environments	656:681	physiological environments	656:681	Release of zinc in physiological environments showed that concentration of zinc released in solution lies below the cytotoxicity level.
25857965	7	47	theme	calcium	924:930	arg1	solution					941:948	a calcium chloride solution	922:948	a calcium chloride solution	922:948	The hydrogel with highest zinc content was stabilized with calcium by immersion in a calcium chloride solution.
25857965	3	48	from	content	317:323	arg1	solution					398:405	normal saline solution	384:405	normal saline solution	384:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	1	49	theme	controlled	122:131	arg1	gelation					133:140	a controlled gelation	120:140	a controlled gelation of alginate	120:152	In this paper, a controlled gelation of alginate was performed for the first time using ZnCO3 and GDL.
25857965	4	50	from	improvement	600:610	arg1	uptake					586:591	water uptake	580:591	water uptake	580:591	Gelation rate increased at increasing zinc content, while a decrease in water uptake and an improvement of stability were found.
25857965	8	51	theme	resulting	955:963	arg1	hydrogel					965:972	The resulting hydrogel	951:972	The resulting hydrogel	951:972	The resulting hydrogel preserved homogeneity and antibacterial activity.
25857965	2	52	theme	transparent	220:230	arg1	gels					232:235	Uniform and transparent gels	208:235	Uniform and transparent gels	208:235	Uniform and transparent gels were obtained and investigated as potential wound dressings.
25857965	2	52	theme	transparent	220:230	arg1	dressings					287:295	potential wound dressings	271:295	potential wound dressings	271:295	Uniform and transparent gels were obtained and investigated as potential wound dressings.
25857965	3	53	from	properties	419:428	arg1	solution					398:405	normal saline solution	384:405	normal saline solution	384:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
25857965	9	54	theme	stability	1070:1078	arg1	improvement					1055:1065	an improvement	1052:1065	an improvement	1052:1065	Furthermore, it showed even an improvement of stability and mechanical properties, which makes it suitable as long-lasting wound dressing.
25857965	3	55	from	Homogeneity	298:308	arg1	solution					398:405	normal saline solution	384:405	normal saline solution	384:405	Homogeneity, water content, swelling capability, water evaporation rate, stability in normal saline solution, mechanical properties and antibacterial activity were assessed as a function of zinc concentration.
27612793	2	0	theme	angle	458:462	arg1	measurements					464:475	contact angle measurements	450:475	contact angle measurements	450:475	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	5	1	theme	surface	1006:1012	arg1	energy					1014:1019	surface energy	1006:1019	surface energy	1006:1019	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	6	2	theme	Gram-negative	1183:1195	arg1	bacteria					1197:1204	Gram-negative bacteria	1183:1204	Gram-negative bacteria	1183:1204	The nanocomposites were also evaluated for antibacterial activity against bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli).
27612793	2	3	theme	contact	450:456	arg1	measurements					464:475	contact angle measurements	450:475	contact angle measurements	450:475	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	7	4	theme	5.2logcolonyformingunits	1357:1380	arg1	decrease					1345:1352	the decrease	1341:1352	the decrease of 5.2logcolonyformingunits (CFU) for B. subtilis	1341:1402	The results indicate that the nanocomposites are effective against all of the bacteria studied as shown by the decrease of 5.2logcolonyformingunits (CFU) for B. subtilis and 6.5logCFU for E. coli.
27612793	7	4	theme	5.2logcolonyformingunits	1357:1380	arg1	6.5logCFU					1408:1416	6.5logCFU	1408:1416	6.5logCFU for E. coli	1408:1428	The results indicate that the nanocomposites are effective against all of the bacteria studied as shown by the decrease of 5.2logcolonyformingunits (CFU) for B. subtilis and 6.5logCFU for E. coli.
27612793	9	5	theme	inhibition	1531:1540	arg1	zone					1542:1545	the resulting inhibition zone	1517:1545	the resulting inhibition zone	1517:1545	The perfect match between the resulting inhibition zone and the composite surface area has demonstrated that our composite was contact active with a slight leaching of PPy.
27612793	9	6	theme	slight	1640:1645	arg1	leaching					1647:1654	a slight leaching	1638:1654	a slight leaching of PPy	1638:1661	The perfect match between the resulting inhibition zone and the composite surface area has demonstrated that our composite was contact active with a slight leaching of PPy.
27612793	3	7	contain	has	564:566	arg2	stress					639:644	tensile stress	631:644	tensile stress	631:644	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	3	7	contain	has	564:566	arg2	3.4GPa					600:605	3.4GPa	600:605	3.4GPa	600:605	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	3	7	contain	has	564:566	arg2	%					624:624	2.6%	621:624	2.6%	621:624	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	3	7	contain	has	564:566	arg1	composite					554:562	The developed composite	540:562	The developed composite	540:562	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	3	7	contain	has	564:566	arg2	elongation					609:618	elongation	609:618	elongation (2.6%)	609:625	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	3	7	contain	has	564:566	arg2	modulus					591:597	nearly identical Young modulus	568:597	nearly identical Young modulus (3.4GPa)	568:606	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	4	8	theme	composite	843:851	arg1	surface					853:859	the composite surface	839:859	the composite surface	839:859	From the energy-dispersive X-ray spectroscopy (EDX) results, it was shown that PPy is mainly located on the composite surface.
27612793	1	9	theme	PPy	317:319	arg1	absorption					303:312	the induced absorption	291:312	the induced absorption of PPy	291:319	In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN.
27612793	11	10	theme	active	1923:1928	arg1	packaging					1938:1946	an active sterile packaging	1920:1946	an active sterile packaging for surgical instruments	1920:1971	While it has not been tested on bacteria found in medicine, TOCN/PVA-PPy film may be able to act as an active sterile packaging for surgical instruments.
27612793	11	10	theme	active	1923:1928	arg1	film					1893:1896	TOCN/PVA-PPy film	1880:1896	TOCN/PVA-PPy film	1880:1896	While it has not been tested on bacteria found in medicine, TOCN/PVA-PPy film may be able to act as an active sterile packaging for surgical instruments.
27612793	5	11	theme	contact	915:921	arg1	angle					923:927	contact angle	915:927	contact angle	915:927	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	9	12	theme	resulting	1521:1529	arg1	zone					1542:1545	the resulting inhibition zone	1517:1545	the resulting inhibition zone	1517:1545	The perfect match between the resulting inhibition zone and the composite surface area has demonstrated that our composite was contact active with a slight leaching of PPy.
27612793	9	13	theme	PPy	1659:1661	arg1	leaching					1647:1654	a slight leaching	1638:1654	a slight leaching of PPy	1638:1661	The perfect match between the resulting inhibition zone and the composite surface area has demonstrated that our composite was contact active with a slight leaching of PPy.
27612793	11	14	theme	sterile	1930:1936	arg1	packaging					1938:1946	an active sterile packaging	1920:1946	an active sterile packaging for surgical instruments	1920:1971	While it has not been tested on bacteria found in medicine, TOCN/PVA-PPy film may be able to act as an active sterile packaging for surgical instruments.
27612793	11	14	theme	sterile	1930:1936	arg1	film					1893:1896	TOCN/PVA-PPy film	1880:1896	TOCN/PVA-PPy film	1880:1896	While it has not been tested on bacteria found in medicine, TOCN/PVA-PPy film may be able to act as an active sterile packaging for surgical instruments.
27612793	10	15	theme	diseases	1810:1817	arg1	spread					1791:1796	the spread	1787:1796	the spread of possible diseases	1787:1817	Our composite was successful as an active packaging on meat (liver) as bacteria were killed by contact, thereby preventing the spread of possible diseases.
27612793	6	16	theme	antibacterial	1065:1077	arg1	activity					1079:1086	antibacterial activity	1065:1086	antibacterial activity against bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli)	1065:1231	The nanocomposites were also evaluated for antibacterial activity against bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli).
27612793	4	17	theme	spectroscopy	768:779	arg1	results					787:793	the energy-dispersive X-ray spectroscopy (EDX) results	740:793	the energy-dispersive X-ray spectroscopy (EDX) results	740:793	From the energy-dispersive X-ray spectroscopy (EDX) results, it was shown that PPy is mainly located on the composite surface.
27612793	5	18	dep	83°	908:910	arg1	to					905:906	to	905:906	to	905:906	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	3	19	dep	has	564:566	arg1	incorporated					721:732	incorporated	721:732	was incorporated	717:732	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	2	20	theme	mechanical	478:487	arg1	tests					489:493	mechanical tests	478:493	mechanical tests	478:493	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	4	21	theme	X-ray	762:766	arg1	EDX					782:784	EDX	782:784	EDX	782:784	From the energy-dispersive X-ray spectroscopy (EDX) results, it was shown that PPy is mainly located on the composite surface.
27612793	4	21	theme	X-ray	762:766	arg1	spectroscopy					768:779	energy-dispersive X-ray spectroscopy	744:779	the energy-dispersive X-ray spectroscopy (EDX) results	740:793	From the energy-dispersive X-ray spectroscopy (EDX) results, it was shown that PPy is mainly located on the composite surface.
27612793	0	22	theme	Mechanical	0:9	arg1	properties					29:38	Mechanical and antibacterial properties	0:38	Mechanical and antibacterial properties of a nanocellulose-polypyrrole multilayer composite	0:90	Mechanical and antibacterial properties of a nanocellulose-polypyrrole multilayer composite.
27612793	4	23	located	located	828:834	arg1	surface					853:859	the composite surface	839:859	the composite surface	839:859	From the energy-dispersive X-ray spectroscopy (EDX) results, it was shown that PPy is mainly located on the composite surface.
27612793	4	23	located	located	828:834	arg2	PPy					814:816	PPy	814:816	PPy	814:816	From the energy-dispersive X-ray spectroscopy (EDX) results, it was shown that PPy is mainly located on the composite surface.
27612793	9	24	with	active	1626:1631	arg1	leaching					1647:1654	a slight leaching	1638:1654	a slight leaching of PPy	1638:1661	The perfect match between the resulting inhibition zone and the composite surface area has demonstrated that our composite was contact active with a slight leaching of PPy.
27612793	9	25	theme	surface	1565:1571	arg1	area					1573:1576	the composite surface area	1551:1576	the composite surface area	1551:1576	The perfect match between the resulting inhibition zone and the composite surface area has demonstrated that our composite was contact active with a slight leaching of PPy.
27612793	3	26	theme	developed	544:552	arg1	composite					554:562	The developed composite	540:562	The developed composite	540:562	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	1	27	theme	polyvinyl	178:186	arg1	PVA					197:199	PVA	197:199	PVA	197:199	In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN.
27612793	1	27	theme	polyvinyl	178:186	arg1	alcohol					188:194	polyvinyl alcohol	178:194	polyvinyl alcohol (PVA)	178:200	In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN.
27612793	2	28	theme	electron	401:408	arg1	microscopy					410:419	scanning electron microscopy	392:419	scanning electron microscopy	392:419	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	11	29	theme	TOCN/PVA-PPy	1880:1891	arg1	packaging					1938:1946	an active sterile packaging	1920:1946	an active sterile packaging for surgical instruments	1920:1971	While it has not been tested on bacteria found in medicine, TOCN/PVA-PPy film may be able to act as an active sterile packaging for surgical instruments.
27612793	11	29	theme	TOCN/PVA-PPy	1880:1891	arg1	film					1893:1896	TOCN/PVA-PPy film	1880:1896	TOCN/PVA-PPy film	1880:1896	While it has not been tested on bacteria found in medicine, TOCN/PVA-PPy film may be able to act as an active sterile packaging for surgical instruments.
27612793	2	30	theme	scanning	392:399	arg1	microscopy					410:419	scanning electron microscopy	392:419	scanning electron microscopy	392:419	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	6	31	located	found	1118:1122	arg1	food					1127:1130	food	1127:1130	food	1127:1130	The nanocomposites were also evaluated for antibacterial activity against bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli).
27612793	6	31	located	found	1118:1122	arg2	bacteria					1096:1103	bacteria	1096:1103	bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli)	1096:1231	The nanocomposites were also evaluated for antibacterial activity against bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli).
27612793	2	32	theme	properties	528:537	arg1	measurements					464:475	contact angle measurements	450:475	contact angle measurements	450:475	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	2	32	theme	properties	528:537	arg1	tests					489:493	mechanical tests	478:493	mechanical tests	478:493	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	2	32	theme	properties	528:537	arg1	evaluation					500:509	evaluation	500:509	evaluation of antibacterial properties	500:537	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	2	32	theme	properties	528:537	arg1	microscopy					410:419	scanning electron microscopy	392:419	scanning electron microscopy	392:419	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	2	32	theme	properties	528:537	arg1	analysis					440:447	thermogravimetric analysis	422:447	thermogravimetric analysis	422:447	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	0	33	theme	antibacterial	15:27	arg1	properties					29:38	Mechanical and antibacterial properties	0:38	Mechanical and antibacterial properties of a nanocellulose-polypyrrole multilayer composite	0:90	Mechanical and antibacterial properties of a nanocellulose-polypyrrole multilayer composite.
27612793	1	34	theme	chemical	253:260	arg1	polymerization					262:275	a chemical polymerization	251:275	a chemical polymerization	251:275	In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN.
27612793	2	35	theme	antibacterial	514:526	arg1	properties					528:537	antibacterial properties	514:537	antibacterial properties	514:537	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	1	36	theme	composite	110:118	arg1	film					120:123	a composite film	108:123	a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy)	108:222	In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN.
27612793	5	37	from	83°	908:910	arg1	increase					886:893	an increase	883:893	an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis)	883:986	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	5	37	from	83°	908:910	arg1	decrease					994:1001	a decrease	992:1001	a decrease in surface energy	992:1019	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	5	37	from	83°	908:910	arg1	angle					923:927	contact angle	915:927	contact angle	915:927	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	11	38	located	found	1861:1865	arg1	medicine					1870:1877	medicine	1870:1877	medicine	1870:1877	While it has not been tested on bacteria found in medicine, TOCN/PVA-PPy film may be able to act as an active sterile packaging for surgical instruments.
27612793	11	38	located	found	1861:1865	arg2	bacteria					1852:1859	bacteria	1852:1859	bacteria found in medicine	1852:1877	While it has not been tested on bacteria found in medicine, TOCN/PVA-PPy film may be able to act as an active sterile packaging for surgical instruments.
27612793	10	39	theme	possible	1801:1808	arg1	diseases					1810:1817	possible diseases	1801:1817	possible diseases	1801:1817	Our composite was successful as an active packaging on meat (liver) as bacteria were killed by contact, thereby preventing the spread of possible diseases.
27612793	1	40	theme	TOCN	343:346	arg1	surface					328:334	the surface	324:334	the surface of the TOCN	324:346	In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN.
27612793	3	41	theme	identical	575:583	arg1	3.4GPa					600:605	3.4GPa	600:605	3.4GPa	600:605	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	3	41	theme	identical	575:583	arg1	modulus					591:597	nearly identical Young modulus	568:597	nearly identical Young modulus (3.4GPa)	568:606	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	10	42	from	packaging	1706:1714	arg1	liver					1725:1729	liver	1725:1729	liver	1725:1729	Our composite was successful as an active packaging on meat (liver) as bacteria were killed by contact, thereby preventing the spread of possible diseases.
27612793	10	42	from	packaging	1706:1714	arg1	meat					1719:1722	meat	1719:1722	meat (liver)	1719:1730	Our composite was successful as an active packaging on meat (liver) as bacteria were killed by contact, thereby preventing the spread of possible diseases.
27612793	3	43	dep	even	668:671	arg1	if					673:674	if	673:674	if	673:674	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	5	44	theme	Thermogravimetric	960:976	arg1	protection					948:957	an increased heat protection	930:957	an increased heat protection (Thermogravimetric analysis)	930:986	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	5	44	theme	Thermogravimetric	960:976	arg1	analysis					978:985	Thermogravimetric analysis	960:985	Thermogravimetric analysis	960:985	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	3	45	theme	Young	585:589	arg1	3.4GPa					600:605	3.4GPa	600:605	3.4GPa	600:605	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	3	45	theme	Young	585:589	arg1	modulus					591:597	nearly identical Young modulus	568:597	nearly identical Young modulus (3.4GPa)	568:606	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	2	46	theme	composite	353:361	arg1	films					363:367	The composite films	349:367	The composite films	349:367	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	2	47	theme	thermogravimetric	422:438	arg1	analysis					440:447	thermogravimetric analysis	422:447	thermogravimetric analysis	422:447	The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties.
27612793	8	48	theme	total	1448:1452	arg1	destruction					1454:1464	the total destruction	1444:1464	the total destruction of the studied bacteria	1444:1488	Resulting in the total destruction of the studied bacteria.
27612793	0	49	theme	composite	82:90	arg1	properties					29:38	Mechanical and antibacterial properties	0:38	Mechanical and antibacterial properties of a nanocellulose-polypyrrole multilayer composite	0:90	Mechanical and antibacterial properties of a nanocellulose-polypyrrole multilayer composite.
27612793	4	50	theme	energy-dispersive	744:760	arg1	EDX					782:784	EDX	782:784	EDX	782:784	From the energy-dispersive X-ray spectroscopy (EDX) results, it was shown that PPy is mainly located on the composite surface.
27612793	4	50	theme	energy-dispersive	744:760	arg1	spectroscopy					768:779	energy-dispersive X-ray spectroscopy	744:779	the energy-dispersive X-ray spectroscopy (EDX) results	740:793	From the energy-dispersive X-ray spectroscopy (EDX) results, it was shown that PPy is mainly located on the composite surface.
27612793	5	51	from	decrease	994:1001	arg1	energy					1014:1019	surface energy	1006:1019	surface energy	1006:1019	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	6	52	dep	bacteria	1096:1103	arg1	bacteria					1197:1204	Gram-negative bacteria	1183:1204	Gram-negative bacteria	1183:1204	The nanocomposites were also evaluated for antibacterial activity against bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli).
27612793	6	52	dep	bacteria	1096:1103	arg1	subtilis					1156:1163	Bacillus subtilis	1147:1163	Bacillus subtilis (B. subtilis)	1147:1177	The nanocomposites were also evaluated for antibacterial activity against bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli).
27612793	0	53	theme	nanocellulose-polypyrrole	45:69	arg1	composite					82:90	a nanocellulose-polypyrrole multilayer composite	43:90	a nanocellulose-polypyrrole multilayer composite	43:90	Mechanical and antibacterial properties of a nanocellulose-polypyrrole multilayer composite.
27612793	9	54	theme	composite	1555:1563	arg1	area					1573:1576	the composite surface area	1551:1576	the composite surface area	1551:1576	The perfect match between the resulting inhibition zone and the composite surface area has demonstrated that our composite was contact active with a slight leaching of PPy.
27612793	3	55	theme	poor	690:693	arg1	which					681:685	which	681:685	which	681:685	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	3	55	theme	poor	690:693	arg1	properties					695:704	poor properties	690:704	poor properties by itself	690:714	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	11	56	theme	surgical	1952:1959	arg1	instruments					1961:1971	surgical instruments	1952:1971	surgical instruments	1952:1971	While it has not been tested on bacteria found in medicine, TOCN/PVA-PPy film may be able to act as an active sterile packaging for surgical instruments.
27612793	3	57	theme	tensile	631:637	arg1	stress					639:644	tensile stress	631:644	tensile stress	631:644	The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated.
27612793	8	58	theme	bacteria	1481:1488	arg1	destruction					1454:1464	the total destruction	1444:1464	the total destruction of the studied bacteria	1444:1488	Resulting in the total destruction of the studied bacteria.
27612793	5	59	theme	increased	933:941	arg1	83°					908:910	83°	908:910	83°	908:910	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	5	59	theme	increased	933:941	arg1	protection					948:957	an increased heat protection	930:957	an increased heat protection (Thermogravimetric analysis)	930:986	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	5	59	theme	increased	933:941	arg1	analysis					978:985	Thermogravimetric analysis	960:985	Thermogravimetric analysis	960:985	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	1	60	theme	TEMPO-oxidized	134:147	arg1	nanofibers					159:168	TEMPO-oxidized cellulose nanofibers	134:168	TEMPO-oxidized cellulose nanofibers (TOCN)	134:175	In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN.
27612793	1	60	theme	TEMPO-oxidized	134:147	arg1	TOCN					171:174	TOCN	171:174	TOCN	171:174	In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN.
27612793	5	61	from	increase	886:893	arg1	energy					1014:1019	surface energy	1006:1019	surface energy	1006:1019	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	6	62	dep	subtilis	1156:1163	arg1	coli					1218:1221	Escherichia coli	1206:1221	Escherichia coli	1206:1221	The nanocomposites were also evaluated for antibacterial activity against bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli).
27612793	6	62	dep	subtilis	1156:1163	arg1	coli					1227:1230	E. coli	1224:1230	E. coli	1224:1230	The nanocomposites were also evaluated for antibacterial activity against bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli).
27612793	6	62	dep	subtilis	1156:1163	arg1	subtilis					1169:1176	B. subtilis	1166:1176	B. subtilis	1166:1176	The nanocomposites were also evaluated for antibacterial activity against bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli).
27612793	5	63	theme	heat	943:946	arg1	83°					908:910	83°	908:910	83°	908:910	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	5	63	theme	heat	943:946	arg1	protection					948:957	an increased heat protection	930:957	an increased heat protection (Thermogravimetric analysis)	930:986	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	5	63	theme	heat	943:946	arg1	analysis					978:985	Thermogravimetric analysis	960:985	Thermogravimetric analysis	960:985	Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy.
27612793	10	64	theme	active	1699:1704	arg1	packaging					1706:1714	an active packaging	1696:1714	an active packaging on meat (liver)	1696:1730	Our composite was successful as an active packaging on meat (liver) as bacteria were killed by contact, thereby preventing the spread of possible diseases.
27612793	1	65	theme	cellulose	149:157	arg1	nanofibers					159:168	TEMPO-oxidized cellulose nanofibers	134:168	TEMPO-oxidized cellulose nanofibers (TOCN)	134:175	In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN.
27612793	1	65	theme	cellulose	149:157	arg1	TOCN					171:174	TOCN	171:174	TOCN	171:174	In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN.
27612793	0	66	theme	multilayer	71:80	arg1	composite					82:90	a nanocellulose-polypyrrole multilayer composite	43:90	a nanocellulose-polypyrrole multilayer composite	43:90	Mechanical and antibacterial properties of a nanocellulose-polypyrrole multilayer composite.
27612793	1	67	theme	induced	295:301	arg1	absorption					303:312	the induced absorption	291:312	the induced absorption of PPy	291:319	In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN.
27612793	8	68	theme	studied	1473:1479	arg1	bacteria					1481:1488	the studied bacteria	1469:1488	the studied bacteria	1469:1488	Resulting in the total destruction of the studied bacteria.
26966997	0	0	theme	3D	81:82	arg1	model					96:100	an in vitro 3D oral mucosa model	69:100	an in vitro 3D oral mucosa model	69:100	Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
26966997	7	1	theme	epithelium	1232:1241	arg1	quality					1204:1210	the quality	1200:1210	the quality of the oral mucosal epithelium	1200:1241	Moreover, upregulation of those markers may play a role in increasing the quality of the oral mucosal epithelium.
26966997	6	2	theme	XPP	1059:1061	arg1	supplementation					1063:1077	XPP supplementation	1059:1077	XPP supplementation	1059:1077	These findings suggest that XPP supplementation contributes to consistent epithelial regeneration.
26966997	5	3	theme	GAG	914:916	arg1	amount					918:923	GAG amount	914:923	GAG amount	914:923	It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates.
26966997	1	4	theme	regenerative	258:269	arg1	medicine					271:278	regenerative medicine	258:278	regenerative medicine	258:278	Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting.
26966997	5	5	theme	composition	932:942	arg1	change					904:909	change	904:909	change of GAG amount and/or composition	904:942	It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates.
26966997	7	6	theme	markers	1162:1168	arg1	upregulation					1140:1151	upregulation	1140:1151	upregulation of those markers	1140:1168	Moreover, upregulation of those markers may play a role in increasing the quality of the oral mucosal epithelium.
26966997	2	7	from	effects	341:347	arg1	regeneration					454:465	oral epithelial regeneration	438:465	oral epithelial regeneration	438:465	This study investigated the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration.
26966997	5	8	theme	amount	918:923	arg1	change					904:909	change	904:909	change of GAG amount and/or composition	904:942	It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates.
26966997	0	9	theme	mucosa	89:94	arg1	model					96:100	an in vitro 3D oral mucosa model	69:100	an in vitro 3D oral mucosa model	69:100	Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
26966997	0	10	from	Effects	0:6	arg1	regeneration					53:64	epithelial regeneration	42:64	epithelial regeneration	42:64	Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
26966997	3	11	theme	glycosaminoglycan	562:578	arg1	synthesis					586:594	glycosaminoglycan (GAG) synthesis	562:594	glycosaminoglycan (GAG) synthesis	562:594	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	5	12	theme	integrin	977:984	arg1	α6					986:987	integrin α6	977:987	integrin α6	977:987	It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates.
26966997	0	13	theme	oral	84:87	arg1	model					96:100	an in vitro 3D oral mucosa model	69:100	an in vitro 3D oral mucosa model	69:100	Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
26966997	5	14	theme	α6	986:987	arg1	expressions					962:972	the expressions	958:972	the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates	958:1028	It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates.
26966997	4	15	theme	epithelial	769:778	arg1	thickness					780:788	the mean and minimal epithelial thickness	748:788	the mean and minimal epithelial thickness	748:788	Compared with the control, 2 mM XPP treatment increased the mean and minimal epithelial thickness, and reduced the variation of epithelial thickness.
26966997	5	16	theme	decorin	876:882	arg1	expressions					861:871	expressions	861:871	expressions of decorin and syndecan-1	861:897	It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates.
26966997	4	17	theme	XPP	724:726	arg1	treatment					728:736	2 mM XPP treatment	719:736	2 mM XPP treatment	719:736	Compared with the control, 2 mM XPP treatment increased the mean and minimal epithelial thickness, and reduced the variation of epithelial thickness.
26966997	3	18	theme	mucosa	499:504	arg1	model					506:510	a three-dimensional oral mucosa model	474:510	a three-dimensional oral mucosa model	474:510	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	19	theme	structure	551:559	arg1	levels					612:617	the expression levels	597:617	the expression levels of basement membrane zone markers	597:651	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	19	theme	structure	551:559	arg1	substrates					658:667	substrates	658:667	substrates of Akt/mTOR signaling	658:689	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	19	theme	structure	551:559	arg1	synthesis					586:594	glycosaminoglycan (GAG) synthesis	562:594	glycosaminoglycan (GAG) synthesis	562:594	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	19	theme	structure	551:559	arg1	changes					525:531	changes	525:531	changes of the epithelial structure	525:559	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	1	20	theme	poor	171:174	arg1	epithelium					176:185	a poor epithelium	169:185	a poor epithelium before clinical use	169:205	Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting.
26966997	5	21	theme	CD44	990:993	arg1	expressions					962:972	the expressions	958:972	the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates	958:1028	It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates.
26966997	6	22	theme	consistent	1094:1103	arg1	regeneration					1116:1127	consistent epithelial regeneration	1094:1127	consistent epithelial regeneration	1094:1127	These findings suggest that XPP supplementation contributes to consistent epithelial regeneration.
26966997	3	23	theme	epithelial	540:549	arg1	structure					551:559	the epithelial structure	536:559	the epithelial structure	536:559	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	6	24	theme	epithelial	1105:1114	arg1	regeneration					1116:1127	consistent epithelial regeneration	1094:1127	consistent epithelial regeneration	1094:1127	These findings suggest that XPP supplementation contributes to consistent epithelial regeneration.
26966997	4	25	theme	mean	752:755	arg1	thickness					780:788	the mean and minimal epithelial thickness	748:788	the mean and minimal epithelial thickness	748:788	Compared with the control, 2 mM XPP treatment increased the mean and minimal epithelial thickness, and reduced the variation of epithelial thickness.
26966997	5	26	theme	syndecan-1	888:897	arg1	expressions					861:871	expressions	861:871	expressions of decorin and syndecan-1	861:897	It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates.
26966997	1	27	theme	clinical	194:201	arg1	use					203:205	clinical use	194:205	clinical use	194:205	Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting.
26966997	0	28	theme	derivative	28:37	arg1	Effects					0:6	Effects	0:6	Effects of C-xylopyranoside derivative on epithelial regeneration	0:64	Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
26966997	5	29	theme	Akt/mTOR	1000:1007	arg1	substrates					1019:1028	Akt/mTOR signaling substrates	1000:1028	Akt/mTOR signaling substrates	1000:1028	It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates.
26966997	0	30	dep	in	72:73	arg1	vitro					75:79	vitro	75:79	vitro	75:79	Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
26966997	5	31	theme	substrates	1019:1028	arg1	expressions					962:972	the expressions	958:972	the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates	958:1028	It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates.
26966997	2	32	theme	oral	438:441	arg1	regeneration					454:465	oral epithelial regeneration	438:465	oral epithelial regeneration	438:465	This study investigated the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration.
26966997	2	33	theme	epithelial	443:452	arg1	regeneration					454:465	oral epithelial regeneration	438:465	oral epithelial regeneration	438:465	This study investigated the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration.
26966997	1	34	theme	mucosa	150:155	arg1	grafts					157:162	substandard tissue-engineered oral mucosa grafts	115:162	substandard tissue-engineered oral mucosa grafts	115:162	Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting.
26966997	0	35	theme	epithelial	42:51	arg1	regeneration					53:64	epithelial regeneration	42:64	epithelial regeneration	42:64	Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
26966997	1	36	theme	grafting	303:310	arg1	success					292:298	success	292:298	success of grafting	292:310	Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting.
26966997	4	37	theme	thickness	831:839	arg1	variation					807:815	the variation	803:815	the variation of epithelial thickness	803:839	Compared with the control, 2 mM XPP treatment increased the mean and minimal epithelial thickness, and reduced the variation of epithelial thickness.
26966997	0	38	theme	C-xylopyranoside	11:26	arg1	derivative					28:37	C-xylopyranoside derivative	11:37	C-xylopyranoside derivative	11:37	Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
26966997	4	39	theme	epithelial	820:829	arg1	thickness					831:839	epithelial thickness	820:839	epithelial thickness	820:839	Compared with the control, 2 mM XPP treatment increased the mean and minimal epithelial thickness, and reduced the variation of epithelial thickness.
26966997	3	40	theme	three-dimensional	476:492	arg1	model					506:510	a three-dimensional oral mucosa model	474:510	a three-dimensional oral mucosa model	474:510	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	7	41	theme	oral	1219:1222	arg1	epithelium					1232:1241	the oral mucosal epithelium	1215:1241	the oral mucosal epithelium	1215:1241	Moreover, upregulation of those markers may play a role in increasing the quality of the oral mucosal epithelium.
26966997	1	42	from	assurance/control	237:253	arg1	medicine					271:278	regenerative medicine	258:278	regenerative medicine	258:278	Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting.
26966997	5	43	theme	signaling	1009:1017	arg1	substrates					1019:1028	Akt/mTOR signaling substrates	1000:1028	Akt/mTOR signaling substrates	1000:1028	It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates.
26966997	2	44	theme	one	352:354	arg1	effects					341:347	the effects	337:347	the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration	337:465	This study investigated the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration.
26966997	3	45	theme	expression	601:610	arg1	levels					612:617	the expression levels	597:617	the expression levels of basement membrane zone markers	597:651	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	4	46	theme	2 mM	719:722	arg1	treatment					728:736	2 mM XPP treatment	719:736	2 mM XPP treatment	719:736	Compared with the control, 2 mM XPP treatment increased the mean and minimal epithelial thickness, and reduced the variation of epithelial thickness.
26966997	3	47	theme	membrane	631:638	arg1	markers					645:651	basement membrane zone markers	622:651	basement membrane zone markers	622:651	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	48	theme	zone	640:643	arg1	markers					645:651	basement membrane zone markers	622:651	basement membrane zone markers	622:651	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	1	49	theme	tissue-engineered	127:143	arg1	grafts					157:162	substandard tissue-engineered oral mucosa grafts	115:162	substandard tissue-engineered oral mucosa grafts	115:162	Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting.
26966997	4	50	theme	minimal	761:767	arg1	thickness					780:788	the mean and minimal epithelial thickness	748:788	the mean and minimal epithelial thickness	748:788	Compared with the control, 2 mM XPP treatment increased the mean and minimal epithelial thickness, and reduced the variation of epithelial thickness.
26966997	3	51	theme	Akt/mTOR	672:679	arg1	signaling					681:689	Akt/mTOR signaling	672:689	Akt/mTOR signaling	672:689	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	52	theme	markers	645:651	arg1	levels					612:617	the expression levels	597:617	the expression levels of basement membrane zone markers	597:651	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	52	theme	markers	645:651	arg1	substrates					658:667	substrates	658:667	substrates of Akt/mTOR signaling	658:689	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	52	theme	markers	645:651	arg1	synthesis					586:594	glycosaminoglycan (GAG) synthesis	562:594	glycosaminoglycan (GAG) synthesis	562:594	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	52	theme	markers	645:651	arg1	changes					525:531	changes	525:531	changes of the epithelial structure	525:559	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	1	53	theme	oral	145:148	arg1	grafts					157:162	substandard tissue-engineered oral mucosa grafts	115:162	substandard tissue-engineered oral mucosa grafts	115:162	Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting.
26966997	1	54	theme	quality	229:235	arg1	assurance/control					237:253	quality assurance/control	229:253	quality assurance/control in regenerative medicine	229:278	Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting.
26966997	0	55	theme	in	72:73	arg1	model					96:100	an in vitro 3D oral mucosa model	69:100	an in vitro 3D oral mucosa model	69:100	Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
26966997	3	56	theme	signaling	681:689	arg1	levels					612:617	the expression levels	597:617	the expression levels of basement membrane zone markers	597:651	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	56	theme	signaling	681:689	arg1	substrates					658:667	substrates	658:667	substrates of Akt/mTOR signaling	658:689	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	56	theme	signaling	681:689	arg1	synthesis					586:594	glycosaminoglycan (GAG) synthesis	562:594	glycosaminoglycan (GAG) synthesis	562:594	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	56	theme	signaling	681:689	arg1	changes					525:531	changes	525:531	changes of the epithelial structure	525:559	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	57	theme	basement	622:629	arg1	markers					645:651	basement membrane zone markers	622:651	basement membrane zone markers	622:651	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	3	58	theme	oral	494:497	arg1	model					506:510	a three-dimensional oral mucosa model	474:510	a three-dimensional oral mucosa model	474:510	Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling.
26966997	2	59	theme	derivatives	380:390	arg1	one					352:354	one	352:354	one	352:354	This study investigated the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration.
26966997	2	59	theme	derivatives	380:390	arg1	β-D-xylopyranoside-n-propane-2-one					393:426	β-D-xylopyranoside-n-propane-2-one	393:426	β-D-xylopyranoside-n-propane-2-one (XPP)	393:432	This study investigated the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration.
26966997	2	59	theme	derivatives	380:390	arg1	derivatives					380:390	the C-xylopyranoside derivatives	359:390	the C-xylopyranoside derivatives	359:390	This study investigated the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration.
26966997	0	60	dep	Effects	0:6	arg1	model					96:100	an in vitro 3D oral mucosa model	69:100	an in vitro 3D oral mucosa model	69:100	Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
26966997	2	61	theme	C-xylopyranoside	363:378	arg1	derivatives					380:390	the C-xylopyranoside derivatives	359:390	the C-xylopyranoside derivatives	359:390	This study investigated the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration.
26966997	7	62	theme	mucosal	1224:1230	arg1	epithelium					1232:1241	the oral mucosal epithelium	1215:1241	the oral mucosal epithelium	1215:1241	Moreover, upregulation of those markers may play a role in increasing the quality of the oral mucosal epithelium.
25466047	0	0	theme	casein	79:84	arg1	micelles					86:93	casein micelles	79:93	casein micelles	79:93	The pressure-induced, lactose-dependent changes in the composition and size of casein micelles.
25466047	5	1	theme	hydrogen	858:865	arg1	bonds					867:871	their hydrogen bonds	852:871	their hydrogen bonds around the micellar fragments	852:901	Lactose inhibits the hydrophobic interactions between the micellar fragments during or after pressure release, through the hydrophilic layer formed by their hydrogen bonds around the micellar fragments.
25466047	5	2	theme	hydrophobic	722:732	arg1	interactions					734:745	the hydrophobic interactions	718:745	the hydrophobic interactions between the micellar fragments during or after pressure release	718:809	Lactose inhibits the hydrophobic interactions between the micellar fragments during or after pressure release, through the hydrophilic layer formed by their hydrogen bonds around the micellar fragments.
25466047	7	3	theme	larger	1086:1091	arg1	micelles					1093:1100	larger micelles	1086:1100	larger micelles	1086:1100	Due to these two functions, lactose inhibited the formation of larger micelles after pressure treatment.
25466047	2	4	from	°C	399:400	arg1	pressure					374:381	high pressure	369:381	high pressure (400 MPa) at 20 °C for 40 min	369:411	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	2	4	from	°C	399:400	arg1	MPa					388:390	400 MPa	384:390	400 MPa	384:390	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	2	5	dep	%	335:335	arg1	w/v					338:340	w/v	338:340	w/v	338:340	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	1	6	theme	action	245:250	arg1	mechanism					232:240	the related mechanism	220:240	the related mechanism of action	220:250	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	1	6	theme	action	245:250	arg1	changes					126:132	the changes	122:132	the changes in the composition and size of casein micelles induced by high-pressure treatment	122:214	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	0	7	theme	micelles	86:93	arg1	size					71:74	size	71:74	size	71:74	The pressure-induced, lactose-dependent changes in the composition and size of casein micelles.
25466047	0	7	theme	micelles	86:93	arg1	composition					55:65	composition	55:65	composition	55:65	The pressure-induced, lactose-dependent changes in the composition and size of casein micelles.
25466047	1	8	from	changes	126:132	arg1	size					157:160	size	157:160	size	157:160	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	1	8	from	changes	126:132	arg1	composition					141:151	composition	141:151	composition	141:151	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	5	9	theme	hydrophilic	824:834	arg1	layer					836:840	the hydrophilic layer	820:840	the hydrophilic layer formed by their hydrogen bonds around the micellar fragments	820:901	Lactose inhibits the hydrophobic interactions between the micellar fragments during or after pressure release, through the hydrophilic layer formed by their hydrogen bonds around the micellar fragments.
25466047	4	10	dep	pressure-induced	621:636	arg1	lactose-dependent					639:655	lactose-dependent	639:655	lactose-dependent	639:655	A mechanism for the pressure-induced, lactose-dependent changes in the casein micelles is proposed.
25466047	5	11	theme	micellar	884:891	arg1	fragments					893:901	the micellar fragments	880:901	the micellar fragments	880:901	Lactose inhibits the hydrophobic interactions between the micellar fragments during or after pressure release, through the hydrophilic layer formed by their hydrogen bonds around the micellar fragments.
25466047	6	12	theme	casein	981:986	arg1	aggregates					988:997	the casein aggregates	977:997	the casein aggregates	977:997	In addition, lactose does not favour the association between calcium and the casein aggregates after pressure release.
25466047	2	13	theme	high	369:372	arg1	pressure					374:381	high pressure	369:381	high pressure (400 MPa) at 20 °C for 40 min	369:411	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	2	13	theme	high	369:372	arg1	MPa					388:390	400 MPa	384:390	400 MPa	384:390	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	0	14	theme	pressure-induced	4:19	arg1	changes					40:46	The pressure-induced, lactose-dependent changes	0:46	The pressure-induced, lactose-dependent changes in the composition and size of casein micelles	0:93	The pressure-induced, lactose-dependent changes in the composition and size of casein micelles.
25466047	0	15	from	changes	40:46	arg1	size					71:74	size	71:74	size	71:74	The pressure-induced, lactose-dependent changes in the composition and size of casein micelles.
25466047	0	15	from	changes	40:46	arg1	composition					55:65	composition	55:65	composition	55:65	The pressure-induced, lactose-dependent changes in the composition and size of casein micelles.
25466047	2	16	theme	pellets	318:324	arg1	Dispersions					271:281	Dispersions	271:281	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose	271:349	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	2	17	theme	micelle	310:316	arg1	pellets					318:324	ultracentrifuged casein micelle pellets	286:324	ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose	286:349	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	1	18	theme	casein	165:170	arg1	micelles					172:179	casein micelles	165:179	casein micelles induced by high-pressure treatment	165:214	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	2	19	theme	casein	303:308	arg1	pellets					318:324	ultracentrifuged casein micelle pellets	286:324	ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose	286:349	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	1	20	theme	micelles	172:179	arg1	size					157:160	size	157:160	size	157:160	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	1	20	theme	micelles	172:179	arg1	composition					141:151	composition	141:151	composition	141:151	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	1	21	dep	composition	141:151	arg1	the					137:139	the	137:139	the	137:139	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	2	22	theme	ultracentrifuged	286:301	arg1	pellets					318:324	ultracentrifuged casein micelle pellets	286:324	ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose	286:349	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	4	23	theme	pressure-induced	621:636	arg1	changes					657:663	the pressure-induced, lactose-dependent changes	617:663	the pressure-induced, lactose-dependent changes in the casein micelles	617:686	A mechanism for the pressure-induced, lactose-dependent changes in the casein micelles is proposed.
25466047	7	24	theme	pressure	1108:1115	arg1	treatment					1117:1125	pressure treatment	1108:1125	pressure treatment	1108:1125	Due to these two functions, lactose inhibited the formation of larger micelles after pressure treatment.
25466047	2	25	theme	%	335:335	arg1	lactose					343:349	0-10% (w/v) lactose	331:349	0-10% (w/v) lactose	331:349	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	1	26	from	mechanism	232:240	arg1	size					157:160	size	157:160	size	157:160	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	1	26	from	mechanism	232:240	arg1	composition					141:151	composition	141:151	composition	141:151	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	0	27	dep	composition	55:65	arg1	the					51:53	the	51:53	the	51:53	The pressure-induced, lactose-dependent changes in the composition and size of casein micelles.
25466047	6	28	theme	pressure	1005:1012	arg1	release					1014:1020	pressure release	1005:1020	pressure release	1005:1020	In addition, lactose does not favour the association between calcium and the casein aggregates after pressure release.
25466047	3	29	theme	pressure	542:549	arg1	treatment					551:559	pressure treatment	542:559	pressure treatment	542:559	The results indicated that the level of non-sedimentable caseins was positively related to the amount of lactose added prior to pressure treatment, and negatively correlated to the size.
25466047	1	30	theme	high-pressure	192:204	arg1	treatment					206:214	high-pressure treatment	192:214	high-pressure treatment	192:214	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	7	31	theme	micelles	1093:1100	arg1	formation					1073:1081	the formation	1069:1081	the formation of larger micelles	1069:1100	Due to these two functions, lactose inhibited the formation of larger micelles after pressure treatment.
25466047	5	32	theme	micellar	759:766	arg1	fragments					768:776	the micellar fragments	755:776	the micellar fragments	755:776	Lactose inhibits the hydrophobic interactions between the micellar fragments during or after pressure release, through the hydrophilic layer formed by their hydrogen bonds around the micellar fragments.
25466047	1	33	theme	lactose	111:117	arg1	effects					100:106	The effects	96:106	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action	96:250	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	2	34	theme	0-10	331:334	arg1	%					335:335	%	335:335	%	335:335	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	1	35	from	effects	100:106	arg1	mechanism					232:240	the related mechanism	220:240	the related mechanism of action	220:250	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	1	35	from	effects	100:106	arg1	changes					126:132	the changes	122:132	the changes in the composition and size of casein micelles induced by high-pressure treatment	122:214	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
25466047	3	36	theme	caseins	471:477	arg1	related					494:500	related	494:500	related	494:500	The results indicated that the level of non-sedimentable caseins was positively related to the amount of lactose added prior to pressure treatment, and negatively correlated to the size.
25466047	3	36	theme	caseins	471:477	arg1	level					445:449	the level	441:449	the level of non-sedimentable caseins	441:477	The results indicated that the level of non-sedimentable caseins was positively related to the amount of lactose added prior to pressure treatment, and negatively correlated to the size.
25466047	4	37	theme	casein	672:677	arg1	micelles					679:686	the casein micelles	668:686	the casein micelles	668:686	A mechanism for the pressure-induced, lactose-dependent changes in the casein micelles is proposed.
25466047	0	38	dep	pressure-induced	4:19	arg1	lactose-dependent					22:38	lactose-dependent	22:38	lactose-dependent	22:38	The pressure-induced, lactose-dependent changes in the composition and size of casein micelles.
25466047	5	39	theme	pressure	794:801	arg1	release					803:809	pressure release	794:809	pressure release	794:809	Lactose inhibits the hydrophobic interactions between the micellar fragments during or after pressure release, through the hydrophilic layer formed by their hydrogen bonds around the micellar fragments.
25466047	3	40	theme	non-sedimentable	454:469	arg1	caseins					471:477	non-sedimentable caseins	454:477	non-sedimentable caseins	454:477	The results indicated that the level of non-sedimentable caseins was positively related to the amount of lactose added prior to pressure treatment, and negatively correlated to the size.
25466047	4	41	from	changes	657:663	arg1	micelles					679:686	the casein micelles	668:686	the casein micelles	668:686	A mechanism for the pressure-induced, lactose-dependent changes in the casein micelles is proposed.
25466047	2	42	with	pellets	318:324	arg1	lactose					343:349	0-10% (w/v) lactose	331:349	0-10% (w/v) lactose	331:349	Dispersions of ultracentrifuged casein micelle pellets with 0-10% (w/v) lactose were subjected to high pressure (400 MPa) at 20 °C for 40 min.
25466047	3	43	theme	lactose	519:525	arg1	amount					509:514	the amount	505:514	the amount of lactose added prior to pressure treatment	505:559	The results indicated that the level of non-sedimentable caseins was positively related to the amount of lactose added prior to pressure treatment, and negatively correlated to the size.
25466047	3	43	theme	lactose	519:525	arg1	lactose					519:525	lactose	519:525	lactose added prior to pressure treatment	519:559	The results indicated that the level of non-sedimentable caseins was positively related to the amount of lactose added prior to pressure treatment, and negatively correlated to the size.
25466047	1	44	theme	related	224:230	arg1	mechanism					232:240	the related mechanism	220:240	the related mechanism of action	220:250	The effects of lactose on the changes in the composition and size of casein micelles induced by high-pressure treatment and the related mechanism of action were investigated.
26822513	0	0	theme	Instar	70:75	arg1	Larvae					77:82	Third Instar Larvae	64:82	Third Instar Larvae	64:82	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
26822513	6	1	theme	identified	1241:1250	arg1	proteins					1252:1259	the identified proteins	1237:1259	the identified proteins	1237:1259	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	1	2	theme	molecular	137:145	arg1	structure					147:155	molecular structure	137:155	molecular structure	137:155	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	4	3	from	RESULTS	639:645	arg1	points					746:751	isoelectric points	734:751	isoelectric points ranging from 3.83 to 11.24	734:778	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	4	3	from	RESULTS	639:645	arg1	total					650:654	total	650:654	total	650:654	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	4	3	from	RESULTS	639:645	arg1	proteins					661:668	374 proteins	657:668	374 proteins	657:668	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	0	4	theme	Third	64:68	arg1	Larvae					77:82	Third Instar Larvae	64:82	Third Instar Larvae	64:82	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
26822513	0	5	from	Analysis	10:17	arg1	Midgut					54:59	the Midgut	50:59	the Midgut of Third Instar Larvae	50:82	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
26822513	5	6	theme	pattern	987:993	arg1	binding					995:1001	pattern binding	987:1001	pattern binding	987:1001	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	3	7	theme	liquid	579:584	arg1	spectrometry					614:625	shotgun liquid chromatography- tandem mass spectrometry	571:625	shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS)	571:636	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	3	7	theme	liquid	579:584	arg1	LC-MS/MS					628:635	LC-MS/MS	628:635	LC-MS/MS	628:635	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	6	8	theme	immune	1319:1324	arg1	system					1326:1331	the housefly larval gut immune system	1295:1331	the housefly larval gut immune system	1295:1331	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	3	9	contain	containing	465:474	arg2	bands					488:492	all protein bands	476:492	all protein bands	476:492	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	3	9	contain	containing	465:474	arg1	1D-PAGE					452:458	A 1D-PAGE lane	450:463	A 1D-PAGE lane containing all protein bands	450:492	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	4	10	theme	most	810:813	arg1	proteins					826:833	most identified proteins	810:833	most identified proteins	810:833	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	0	11	dep	domestica	91:99	arg1	Analysis					10:17	Proteomic Analysis	0:17	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae	0:82	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
26822513	4	12	theme	molecular	676:684	arg1	weights					686:692	molecular weights	676:692	molecular weights varying from 8.225 kD to 996.065 kD	676:728	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	1	13	theme	mass	234:237	arg1	approach					252:259	a mass spectrometry approach	232:259	a mass spectrometry approach	232:259	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	0	14	theme	Larvae	77:82	arg1	Midgut					54:59	the Midgut	50:59	the Midgut of Third Instar Larvae	50:82	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
26822513	5	15	theme	structural	1028:1037	arg1	constituent					1039:1049	structural constituent	1028:1049	structural constituent of peritrophic membrane	1028:1073	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	6	16	dep	CONCLUSION	1134:1143	arg1	composition					1160:1170	The PM protein composition	1145:1170	The PM protein composition	1145:1170	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	6	16	dep	CONCLUSION	1134:1143	arg1	useful					1270:1275	useful	1270:1275	useful	1270:1275	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	6	16	dep	CONCLUSION	1134:1143	arg1	help					1341:1344	help	1341:1344	may help to identify potential targets and exploit new bioinsecticides	1337:1406	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	1	17	theme	spectrometry	239:250	arg1	approach					252:259	a mass spectrometry approach	232:259	a mass spectrometry approach	232:259	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	6	18	theme	larval	1308:1313	arg1	system					1326:1331	the housefly larval gut immune system	1295:1331	the housefly larval gut immune system	1295:1331	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	1	19	used	used	265:268	arg2	approach					252:259	a mass spectrometry approach	232:259	a mass spectrometry approach	232:259	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	6	20	theme	functional	1208:1217	arg1	investigations					1219:1232	further functional investigations	1200:1232	further functional investigations of the identified proteins	1200:1259	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	2	21	theme	larvae	378:383	arg1	midgut					351:356	the midgut	347:356	the midgut of the third instar larvae	347:383	METHODS The PM was dissected from the midgut of the third instar larvae, and protein extracted from the PM was evaluated using SDS-PAGE.
26822513	3	22	theme	protein	480:486	arg1	bands					488:492	all protein bands	476:492	all protein bands	476:492	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	0	23	theme	Musca	85:89	arg1	domestica					91:99	Musca domestica	85:99	Musca domestica	85:99	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
26822513	5	24	theme	membrane	1066:1073	arg1	binding					995:1001	pattern binding	987:1001	pattern binding	987:1001	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	5	24	theme	membrane	1066:1073	arg1	binding					1019:1025	polysaccharide binding	1004:1025	polysaccharide binding	1004:1025	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	5	24	theme	membrane	1066:1073	arg1	constituent					1039:1049	structural constituent	1028:1049	structural constituent of peritrophic membrane	1028:1073	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	5	24	theme	membrane	1066:1073	arg1	binding					1086:1092	chitin binding	1079:1092	chitin binding	1079:1092	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	4	25	dep	11.24	774:778	arg1	to					771:772	to	771:772	to	771:772	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	1	26	theme	physiological	161:173	arg1	function					175:182	physiological function	161:182	physiological function	161:182	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	5	27	theme	peritrophic	1054:1064	arg1	membrane					1066:1073	peritrophic membrane	1054:1073	peritrophic membrane	1054:1073	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	3	28	dep	1D-PAGE	452:458	arg1	lane					460:463	lane	460:463	lane	460:463	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	5	29	theme	Gene	1108:1111	arg1	annotation					1122:1131	Gene Ontology annotation	1108:1131	Gene Ontology annotation	1108:1131	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	0	30	theme	Proteomic	0:8	arg1	Analysis					10:17	Proteomic Analysis	0:17	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae	0:82	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
26822513	5	31	theme	Ontology	1113:1120	arg1	annotation					1122:1131	Gene Ontology annotation	1108:1131	Gene Ontology annotation	1108:1131	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	1	32	dep	structure	147:155	arg1	the					133:135	the	133:135	the	133:135	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	3	33	theme	tandem	602:607	arg1	spectrometry					614:625	shotgun liquid chromatography- tandem mass spectrometry	571:625	shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS)	571:636	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	3	33	theme	tandem	602:607	arg1	LC-MS/MS					628:635	LC-MS/MS	628:635	LC-MS/MS	628:635	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	6	34	theme	PM	1149:1150	arg1	composition					1160:1170	The PM protein composition	1145:1170	The PM protein composition	1145:1170	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	6	34	theme	PM	1149:1150	arg1	useful					1270:1275	useful	1270:1275	useful	1270:1275	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	6	35	theme	housefly	1299:1306	arg1	system					1326:1331	the housefly larval gut immune system	1295:1331	the housefly larval gut immune system	1295:1331	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	3	36	theme	chromatography-	586:600	arg1	spectrometry					614:625	shotgun liquid chromatography- tandem mass spectrometry	571:625	shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS)	571:636	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	3	36	theme	chromatography-	586:600	arg1	LC-MS/MS					628:635	LC-MS/MS	628:635	LC-MS/MS	628:635	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	2	37	dep	METHODS	313:319	arg1	PM					325:326	The PM	321:326	METHODS The PM	313:326	METHODS The PM was dissected from the midgut of the third instar larvae, and protein extracted from the PM was evaluated using SDS-PAGE.
26822513	3	38	dep	bottom	514:519	arg1	top					507:509	top	507:509	top	507:509	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	1	39	theme	housefly	191:198	arg1	PM					227:228	PM	227:228	PM	227:228	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	1	39	theme	housefly	191:198	arg1	matrix					219:224	the housefly larval peritrophic matrix	187:224	the housefly larval peritrophic matrix (PM)	187:229	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	0	40	theme	Matrix	38:43	arg1	Analysis					10:17	Proteomic Analysis	0:17	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae	0:82	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
26822513	2	41	theme	instar	371:376	arg1	larvae					378:383	the third instar larvae	361:383	the third instar larvae	361:383	METHODS The PM was dissected from the midgut of the third instar larvae, and protein extracted from the PM was evaluated using SDS-PAGE.
26822513	1	42	theme	larval	200:205	arg1	PM					227:228	PM	227:228	PM	227:228	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	1	42	theme	larval	200:205	arg1	matrix					219:224	the housefly larval peritrophic matrix	187:224	the housefly larval peritrophic matrix (PM)	187:229	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	0	43	theme	Peritrophic	26:36	arg1	Matrix					38:43	the Peritrophic Matrix	22:43	the Peritrophic Matrix from the Midgut of Third Instar Larvae	22:82	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
26822513	6	44	theme	potential	1358:1366	arg1	targets					1368:1374	potential targets	1358:1374	potential targets	1358:1374	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	3	45	theme	shotgun	571:577	arg1	spectrometry					614:625	shotgun liquid chromatography- tandem mass spectrometry	571:625	shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS)	571:636	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	3	45	theme	shotgun	571:577	arg1	LC-MS/MS					628:635	LC-MS/MS	628:635	LC-MS/MS	628:635	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	4	46	theme	isoelectric	734:744	arg1	points					746:751	isoelectric points	734:751	isoelectric points ranging from 3.83 to 11.24	734:778	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	1	47	theme	peritrophic	207:217	arg1	PM					227:228	PM	227:228	PM	227:228	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	1	47	theme	peritrophic	207:217	arg1	matrix					219:224	the housefly larval peritrophic matrix	187:224	the housefly larval peritrophic matrix (PM)	187:229	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	0	48	from	Midgut	54:59	arg1	Matrix					38:43	the Peritrophic Matrix	22:43	the Peritrophic Matrix from the Midgut of Third Instar Larvae	22:82	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
26822513	0	48	from	Midgut	54:59	arg1	Analysis					10:17	Proteomic Analysis	0:17	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae	0:82	Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae, Musca domestica.
26822513	6	49	theme	new	1388:1390	arg1	bioinsecticides					1392:1406	new bioinsecticides	1388:1406	new bioinsecticides	1388:1406	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	5	50	theme	chitin	1079:1084	arg1	binding					1086:1092	chitin binding	1079:1092	chitin binding	1079:1092	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	4	51	with	total	650:654	arg1	weights					686:692	molecular weights	676:692	molecular weights varying from 8.225 kD to 996.065 kD	676:728	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	1	52	theme	PM	289:290	arg1	composition					300:310	the PM protein composition	285:310	the PM protein composition	285:310	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	4	53	theme	identified	815:824	arg1	proteins					826:833	most identified proteins	810:833	most identified proteins	810:833	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	6	54	theme	further	1200:1206	arg1	investigations					1219:1232	further functional investigations	1200:1232	further functional investigations of the identified proteins	1200:1259	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	1	55	theme	protein	292:298	arg1	composition					300:310	the PM protein composition	285:310	the PM protein composition	285:310	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	5	56	theme	proteins	944:951	arg1	many					930:933	many	930:933	many	930:933	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	5	56	theme	proteins	944:951	arg1	proteins					944:951	these proteins	938:951	these proteins	938:951	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	1	57	theme	matrix	219:224	arg1	function					175:182	physiological function	161:182	physiological function	161:182	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	1	57	theme	matrix	219:224	arg1	structure					147:155	molecular structure	137:155	molecular structure	137:155	OBJECTIVE To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix (PM), a mass spectrometry approach was used to investigate the PM protein composition.
26822513	6	58	theme	proteins	1252:1259	arg1	investigations					1219:1232	further functional investigations	1200:1232	further functional investigations of the identified proteins	1200:1259	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	3	59	theme	mass	609:612	arg1	spectrometry					614:625	shotgun liquid chromatography- tandem mass spectrometry	571:625	shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS)	571:636	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	3	59	theme	mass	609:612	arg1	LC-MS/MS					628:635	LC-MS/MS	628:635	LC-MS/MS	628:635	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	6	60	theme	protein	1152:1158	arg1	composition					1160:1170	The PM protein composition	1145:1170	The PM protein composition	1145:1170	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	6	60	theme	protein	1152:1158	arg1	useful					1270:1275	useful	1270:1275	useful	1270:1275	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26822513	5	61	theme	polysaccharide	1004:1017	arg1	binding					1019:1025	polysaccharide binding	1004:1025	polysaccharide binding	1004:1025	Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation.
26822513	4	62	theme	374	657:659	arg1	proteins					661:668	374 proteins	657:668	374 proteins	657:668	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	4	63	theme	PM	903:904	arg1	structure					906:914	PM structure	903:914	PM structure	903:914	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	2	64	theme	third	365:369	arg1	larvae					378:383	the third instar larvae	361:383	the third instar larvae	361:383	METHODS The PM was dissected from the midgut of the third instar larvae, and protein extracted from the PM was evaluated using SDS-PAGE.
26822513	3	65	theme	trypsinised	542:552	arg1	proteins					526:533	the proteins	522:533	the proteins in-gel trypsinised	522:552	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	3	65	theme	trypsinised	542:552	arg1	bottom					514:519	bottom	514:519	bottom	514:519	A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry (LC-MS/MS).
26822513	4	66	with	points	746:751	arg1	weights					686:692	molecular weights	676:692	molecular weights varying from 8.225 kD to 996.065 kD	676:728	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	4	67	with	proteins	661:668	arg1	weights					686:692	molecular weights	676:692	molecular weights varying from 8.225 kD to 996.065 kD	676:728	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	4	68	theme	nutrient	879:886	arg1	metabolism					888:897	nutrient metabolism	879:897	nutrient metabolism	879:897	RESULTS In total, 374 proteins, with molecular weights varying from 8.225 kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure.
26822513	6	69	theme	gut	1315:1317	arg1	system					1326:1331	the housefly larval gut immune system	1295:1331	the housefly larval gut immune system	1295:1331	CONCLUSION The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.
26838881	7	0	theme	biocompatibility	1139:1154	arg1	studies					1156:1162	In vitro and in vivo biocompatibility studies	1118:1162	In vitro and in vivo biocompatibility studies	1118:1162	In vitro and in vivo biocompatibility studies proved that the gels were biocompatible.
26838881	1	1	theme	articular	314:322	arg1	depot					338:342	visco-supplement and intra articular drug delivery depot	287:342	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis	271:397	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	9	2	theme	In	1319:1320	arg1	results					1348:1354	In vivo fluorescence imaging results	1319:1354	In vivo fluorescence imaging results	1319:1354	In vivo fluorescence imaging results confirmed that CICGs could stay longer in the joint region when compared to BCGs and MPs.
26838881	1	3	theme	Chitosan	247:254	arg1	CICGs					261:265	composite injectable Chitosan gel (CICGs)	226:266	composite injectable Chitosan gel (CICGs)	226:266	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	1	4	theme	drug	324:327	arg1	depot					338:342	visco-supplement and intra articular drug delivery depot	287:342	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis	271:397	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	5	5	theme	Rheology	861:868	arg1	studies					870:876	Rheology studies	861:876	Rheology studies	861:876	CICGs were prepared by dispersing MPs in the chitosan-Ammonium hydrogen phosphate solution and incubated at 37 °C. Rheology studies proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements.
26838881	1	6	theme	gel	256:258	arg1	CICGs					261:265	composite injectable Chitosan gel (CICGs)	226:266	composite injectable Chitosan gel (CICGs)	226:266	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	7	7	theme	in	1131:1132	arg1	studies					1156:1162	In vitro and in vivo biocompatibility studies	1118:1162	In vitro and in vivo biocompatibility studies	1118:1162	In vitro and in vivo biocompatibility studies proved that the gels were biocompatible.
26838881	1	8	theme	delivery	329:336	arg1	depot					338:342	visco-supplement and intra articular drug delivery depot	287:342	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis	271:397	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	5	9	theme	visco-elastic	915:927	arg1	properties					929:938	visco-elastic properties	915:938	visco-elastic properties comparable to that of existing visco-supplements	915:987	CICGs were prepared by dispersing MPs in the chitosan-Ammonium hydrogen phosphate solution and incubated at 37 °C. Rheology studies proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements.
26838881	0	10	theme	etoricoxib	91:100	arg1	formulation					76:86	injectable formulation	65:86	injectable formulation of etoricoxib for the potential treatment of osteoarthritis	65:146	Poly (caprolactone) microparticles and chitosan thermogels based injectable formulation of etoricoxib for the potential treatment of osteoarthritis.
26838881	1	11	theme	depot	338:342	arg1	microparticles					198:211	Poly (caprolactone) microparticles	178:211	Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs)	178:266	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	1	11	theme	depot	338:342	arg1	agent					357:361	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent	271:361	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis	271:397	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	7	12	dep	In	1118:1119	arg1	vitro					1121:1125	vitro	1121:1125	vitro	1121:1125	In vitro and in vivo biocompatibility studies proved that the gels were biocompatible.
26838881	8	13	theme	better	1269:1274	arg1	capacity					1291:1298	a better drug retention capacity	1267:1298	a better drug retention capacity	1267:1298	In vivo synovial drug clearance studies proved that CICGs had a better drug retention capacity than BCGs and MPs.
26838881	0	14	theme	potential	110:118	arg1	treatment					120:128	the potential treatment	106:128	the potential treatment of osteoarthritis	106:146	Poly (caprolactone) microparticles and chitosan thermogels based injectable formulation of etoricoxib for the potential treatment of osteoarthritis.
26838881	5	15	from	MPs	780:782	arg1	solution					828:835	the chitosan-Ammonium hydrogen phosphate solution	787:835	the chitosan-Ammonium hydrogen phosphate solution	787:835	CICGs were prepared by dispersing MPs in the chitosan-Ammonium hydrogen phosphate solution and incubated at 37 °C. Rheology studies proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements.
26838881	4	16	theme	toxicity	729:736	arg1	issues					738:743	these toxicity issues	723:743	these toxicity issues	723:743	So, we have attempted to deliver this drug intra-articularly, which could retain the drug longer in the joint region and thus could ameliorate these toxicity issues.
26838881	5	17	theme	phosphate	818:826	arg1	solution					828:835	the chitosan-Ammonium hydrogen phosphate solution	787:835	the chitosan-Ammonium hydrogen phosphate solution	787:835	CICGs were prepared by dispersing MPs in the chitosan-Ammonium hydrogen phosphate solution and incubated at 37 °C. Rheology studies proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements.
26838881	2	18	theme	model	472:476	arg1	drug					478:481	a model drug	470:481	a model drug for the study	470:495	Etoricoxib (COX-2 inhibitor), a highly hydrophobic drug was chosen as a model drug for the study.
26838881	2	18	theme	model	472:476	arg1	Etoricoxib					400:409	Etoricoxib	400:409	Etoricoxib (COX-2 inhibitor)	400:427	Etoricoxib (COX-2 inhibitor), a highly hydrophobic drug was chosen as a model drug for the study.
26838881	1	19	theme	therapeutic	345:355	arg1	microparticles					198:211	Poly (caprolactone) microparticles	178:211	Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs)	178:266	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	1	19	theme	therapeutic	345:355	arg1	agent					357:361	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent	271:361	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis	271:397	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	9	20	theme	imaging	1340:1346	arg1	results					1348:1354	In vivo fluorescence imaging results	1319:1354	In vivo fluorescence imaging results	1319:1354	In vivo fluorescence imaging results confirmed that CICGs could stay longer in the joint region when compared to BCGs and MPs.
26838881	7	21	theme	In	1118:1119	arg1	studies					1156:1162	In vitro and in vivo biocompatibility studies	1118:1162	In vitro and in vivo biocompatibility studies	1118:1162	In vitro and in vivo biocompatibility studies proved that the gels were biocompatible.
26838881	1	22	theme	Poly	178:181	arg1	microparticles					198:211	Poly (caprolactone) microparticles	178:211	Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs)	178:266	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	1	22	theme	Poly	178:181	arg1	MPs					214:216	MPs	214:216	MPs	214:216	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	1	22	theme	Poly	178:181	arg1	agent					357:361	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent	271:361	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis	271:397	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	6	23	dep	in	994:995	arg1	vitro					997:1001	vitro	997:1001	vitro	997:1001	The in vitro drug release profiles of CICGs were found to be more controlled when compared to MPs and bare chitosan gel (BCGs).
26838881	10	24	theme	potential	1479:1487	arg1	CICGs					1462:1466	this novel CICGs	1451:1466	this novel CICGs	1451:1466	Thus this novel CICGs could be a potential dual purpose gel for the treatment of diseased joint regions especially for Osteoarthritis.
26838881	10	24	theme	potential	1479:1487	arg1	gel					1502:1504	a potential dual purpose gel	1477:1504	a potential dual purpose gel for the treatment of diseased joint regions	1477:1548	Thus this novel CICGs could be a potential dual purpose gel for the treatment of diseased joint regions especially for Osteoarthritis.
26838881	5	25	dep	°C.	857:859	arg1	proved					878:883	proved	878:883	proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements	878:987	CICGs were prepared by dispersing MPs in the chitosan-Ammonium hydrogen phosphate solution and incubated at 37 °C. Rheology studies proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements.
26838881	0	26	theme	Poly	0:3	arg1	microparticles					20:33	Poly (caprolactone) microparticles	0:33	Poly (caprolactone) microparticles	0:33	Poly (caprolactone) microparticles and chitosan thermogels based injectable formulation of etoricoxib for the potential treatment of osteoarthritis.
26838881	8	27	dep	In	1205:1206	arg1	vivo					1208:1211	vivo	1208:1211	vivo	1208:1211	In vivo synovial drug clearance studies proved that CICGs had a better drug retention capacity than BCGs and MPs.
26838881	4	28	theme	joint	684:688	arg1	region					690:695	the joint region	680:695	the joint region	680:695	So, we have attempted to deliver this drug intra-articularly, which could retain the drug longer in the joint region and thus could ameliorate these toxicity issues.
26838881	5	29	contain	had	911:913	arg2	properties					929:938	visco-elastic properties	915:938	visco-elastic properties comparable to that of existing visco-supplements	915:987	CICGs were prepared by dispersing MPs in the chitosan-Ammonium hydrogen phosphate solution and incubated at 37 °C. Rheology studies proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements.
26838881	5	29	contain	had	911:913	arg1	gels					890:893	gels	890:893	gels	890:893	CICGs were prepared by dispersing MPs in the chitosan-Ammonium hydrogen phosphate solution and incubated at 37 °C. Rheology studies proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements.
26838881	6	30	theme	CICGs	1028:1032	arg1	profiles					1016:1023	The in vitro drug release profiles	990:1023	The in vitro drug release profiles of CICGs	990:1032	The in vitro drug release profiles of CICGs were found to be more controlled when compared to MPs and bare chitosan gel (BCGs).
26838881	8	31	contain	had	1263:1265	arg1	CICGs					1257:1261	CICGs	1257:1261	CICGs	1257:1261	In vivo synovial drug clearance studies proved that CICGs had a better drug retention capacity than BCGs and MPs.
26838881	8	31	contain	had	1263:1265	arg2	capacity					1291:1298	a better drug retention capacity	1267:1298	a better drug retention capacity	1267:1298	In vivo synovial drug clearance studies proved that CICGs had a better drug retention capacity than BCGs and MPs.
26838881	6	32	theme	chitosan	1097:1104	arg1	BCGs					1111:1114	bare chitosan gel (BCGs)	1092:1115	bare chitosan gel (BCGs)	1092:1115	The in vitro drug release profiles of CICGs were found to be more controlled when compared to MPs and bare chitosan gel (BCGs).
26838881	8	33	theme	In	1205:1206	arg1	studies					1237:1243	In vivo synovial drug clearance studies	1205:1243	In vivo synovial drug clearance studies	1205:1243	In vivo synovial drug clearance studies proved that CICGs had a better drug retention capacity than BCGs and MPs.
26838881	2	34	theme	COX-2	412:416	arg1	Etoricoxib					400:409	Etoricoxib	400:409	Etoricoxib (COX-2 inhibitor)	400:427	Etoricoxib (COX-2 inhibitor), a highly hydrophobic drug was chosen as a model drug for the study.
26838881	2	34	theme	COX-2	412:416	arg1	inhibitor					418:426	COX-2 inhibitor	412:426	COX-2 inhibitor	412:426	Etoricoxib (COX-2 inhibitor), a highly hydrophobic drug was chosen as a model drug for the study.
26838881	9	35	theme	joint	1402:1406	arg1	region					1408:1413	the joint region	1398:1413	the joint region	1398:1413	In vivo fluorescence imaging results confirmed that CICGs could stay longer in the joint region when compared to BCGs and MPs.
26838881	5	36	theme	comparable	940:949	arg1	properties					929:938	visco-elastic properties	915:938	visco-elastic properties comparable to that of existing visco-supplements	915:987	CICGs were prepared by dispersing MPs in the chitosan-Ammonium hydrogen phosphate solution and incubated at 37 °C. Rheology studies proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements.
26838881	6	37	theme	bare	1092:1095	arg1	BCGs					1111:1114	bare chitosan gel (BCGs)	1092:1115	bare chitosan gel (BCGs)	1092:1115	The in vitro drug release profiles of CICGs were found to be more controlled when compared to MPs and bare chitosan gel (BCGs).
26838881	10	38	theme	novel	1456:1460	arg1	CICGs					1462:1466	this novel CICGs	1451:1466	this novel CICGs	1451:1466	Thus this novel CICGs could be a potential dual purpose gel for the treatment of diseased joint regions especially for Osteoarthritis.
26838881	10	38	theme	novel	1456:1460	arg1	gel					1502:1504	a potential dual purpose gel	1477:1504	a potential dual purpose gel for the treatment of diseased joint regions	1477:1548	Thus this novel CICGs could be a potential dual purpose gel for the treatment of diseased joint regions especially for Osteoarthritis.
26838881	6	39	theme	gel	1106:1108	arg1	BCGs					1111:1114	bare chitosan gel (BCGs)	1092:1115	bare chitosan gel (BCGs)	1092:1115	The in vitro drug release profiles of CICGs were found to be more controlled when compared to MPs and bare chitosan gel (BCGs).
26838881	10	40	theme	joint	1536:1540	arg1	regions					1542:1548	diseased joint regions	1527:1548	diseased joint regions	1527:1548	Thus this novel CICGs could be a potential dual purpose gel for the treatment of diseased joint regions especially for Osteoarthritis.
26838881	1	41	theme	dual	273:276	arg1	purpose					278:284	dual purpose	273:284	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis	271:397	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	6	42	theme	release	1008:1014	arg1	profiles					1016:1023	The in vitro drug release profiles	990:1023	The in vitro drug release profiles of CICGs	990:1032	The in vitro drug release profiles of CICGs were found to be more controlled when compared to MPs and bare chitosan gel (BCGs).
26838881	3	43	theme	toxicity	563:570	arg1	issues					572:577	severe cardiovascular toxicity issues	541:577	severe cardiovascular toxicity issues	541:577	When administered orally, Etoricoxib poses severe cardiovascular toxicity issues.
26838881	10	44	theme	regions	1542:1548	arg1	treatment					1514:1522	the treatment	1510:1522	the treatment of diseased joint regions	1510:1548	Thus this novel CICGs could be a potential dual purpose gel for the treatment of diseased joint regions especially for Osteoarthritis.
26838881	5	45	theme	chitosan-Ammonium	791:807	arg1	solution					828:835	the chitosan-Ammonium hydrogen phosphate solution	787:835	the chitosan-Ammonium hydrogen phosphate solution	787:835	CICGs were prepared by dispersing MPs in the chitosan-Ammonium hydrogen phosphate solution and incubated at 37 °C. Rheology studies proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements.
26838881	2	46	theme	hydrophobic	439:449	arg1	Etoricoxib					400:409	Etoricoxib	400:409	Etoricoxib (COX-2 inhibitor)	400:427	Etoricoxib (COX-2 inhibitor), a highly hydrophobic drug was chosen as a model drug for the study.
26838881	2	46	theme	hydrophobic	439:449	arg1	drug					451:454	a highly hydrophobic drug	430:454	a highly hydrophobic drug	430:454	Etoricoxib (COX-2 inhibitor), a highly hydrophobic drug was chosen as a model drug for the study.
26838881	1	47	theme	purpose	278:284	arg1	microparticles					198:211	Poly (caprolactone) microparticles	178:211	Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs)	178:266	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	1	47	theme	purpose	278:284	arg1	agent					357:361	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent	271:361	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis	271:397	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	0	48	theme	osteoarthritis	133:146	arg1	treatment					120:128	the potential treatment	106:128	the potential treatment of osteoarthritis	106:146	Poly (caprolactone) microparticles and chitosan thermogels based injectable formulation of etoricoxib for the potential treatment of osteoarthritis.
26838881	8	49	theme	synovial	1213:1220	arg1	studies					1237:1243	In vivo synovial drug clearance studies	1205:1243	In vivo synovial drug clearance studies	1205:1243	In vivo synovial drug clearance studies proved that CICGs had a better drug retention capacity than BCGs and MPs.
26838881	5	50	theme	hydrogen	809:816	arg1	solution					828:835	the chitosan-Ammonium hydrogen phosphate solution	787:835	the chitosan-Ammonium hydrogen phosphate solution	787:835	CICGs were prepared by dispersing MPs in the chitosan-Ammonium hydrogen phosphate solution and incubated at 37 °C. Rheology studies proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements.
26838881	10	51	theme	dual	1489:1492	arg1	CICGs					1462:1466	this novel CICGs	1451:1466	this novel CICGs	1451:1466	Thus this novel CICGs could be a potential dual purpose gel for the treatment of diseased joint regions especially for Osteoarthritis.
26838881	10	51	theme	dual	1489:1492	arg1	gel					1502:1504	a potential dual purpose gel	1477:1504	a potential dual purpose gel for the treatment of diseased joint regions	1477:1548	Thus this novel CICGs could be a potential dual purpose gel for the treatment of diseased joint regions especially for Osteoarthritis.
26838881	9	52	dep	In	1319:1320	arg1	vivo					1322:1325	vivo	1322:1325	vivo	1322:1325	In vivo fluorescence imaging results confirmed that CICGs could stay longer in the joint region when compared to BCGs and MPs.
26838881	3	53	theme	severe	541:546	arg1	issues					572:577	severe cardiovascular toxicity issues	541:577	severe cardiovascular toxicity issues	541:577	When administered orally, Etoricoxib poses severe cardiovascular toxicity issues.
26838881	8	54	theme	clearance	1227:1235	arg1	studies					1237:1243	In vivo synovial drug clearance studies	1205:1243	In vivo synovial drug clearance studies	1205:1243	In vivo synovial drug clearance studies proved that CICGs had a better drug retention capacity than BCGs and MPs.
26838881	0	55	theme	chitosan	39:46	arg1	thermogels					48:57	chitosan thermogels	39:57	chitosan thermogels	39:57	Poly (caprolactone) microparticles and chitosan thermogels based injectable formulation of etoricoxib for the potential treatment of osteoarthritis.
26838881	1	56	theme	visco-supplement	287:302	arg1	depot					338:342	visco-supplement and intra articular drug delivery depot	287:342	a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis	271:397	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	1	57	theme	Osteoarthritis	384:397	arg1	treatment					371:379	the treatment	367:379	the treatment of Osteoarthritis	367:397	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	3	58	theme	cardiovascular	548:561	arg1	issues					572:577	severe cardiovascular toxicity issues	541:577	severe cardiovascular toxicity issues	541:577	When administered orally, Etoricoxib poses severe cardiovascular toxicity issues.
26838881	8	59	theme	drug	1222:1225	arg1	studies					1237:1243	In vivo synovial drug clearance studies	1205:1243	In vivo synovial drug clearance studies	1205:1243	In vivo synovial drug clearance studies proved that CICGs had a better drug retention capacity than BCGs and MPs.
26838881	7	60	dep	in	1131:1132	arg1	vivo					1134:1137	vivo	1134:1137	vivo	1134:1137	In vitro and in vivo biocompatibility studies proved that the gels were biocompatible.
26838881	8	61	theme	retention	1281:1289	arg1	capacity					1291:1298	a better drug retention capacity	1267:1298	a better drug retention capacity	1267:1298	In vivo synovial drug clearance studies proved that CICGs had a better drug retention capacity than BCGs and MPs.
26838881	8	62	theme	drug	1276:1279	arg1	capacity					1291:1298	a better drug retention capacity	1267:1298	a better drug retention capacity	1267:1298	In vivo synovial drug clearance studies proved that CICGs had a better drug retention capacity than BCGs and MPs.
26838881	6	63	theme	in	994:995	arg1	profiles					1016:1023	The in vitro drug release profiles	990:1023	The in vitro drug release profiles of CICGs	990:1032	The in vitro drug release profiles of CICGs were found to be more controlled when compared to MPs and bare chitosan gel (BCGs).
26838881	0	64	theme	injectable	65:74	arg1	formulation					76:86	injectable formulation	65:86	injectable formulation of etoricoxib for the potential treatment of osteoarthritis	65:146	Poly (caprolactone) microparticles and chitosan thermogels based injectable formulation of etoricoxib for the potential treatment of osteoarthritis.
26838881	6	65	theme	drug	1003:1006	arg1	profiles					1016:1023	The in vitro drug release profiles	990:1023	The in vitro drug release profiles of CICGs	990:1032	The in vitro drug release profiles of CICGs were found to be more controlled when compared to MPs and bare chitosan gel (BCGs).
26838881	5	66	theme	existing	962:969	arg1	visco-supplements					971:987	existing visco-supplements	962:987	existing visco-supplements	962:987	CICGs were prepared by dispersing MPs in the chitosan-Ammonium hydrogen phosphate solution and incubated at 37 °C. Rheology studies proved that gels were stable and had visco-elastic properties comparable to that of existing visco-supplements.
26838881	1	67	theme	composite	226:234	arg1	CICGs					261:265	composite injectable Chitosan gel (CICGs)	226:266	composite injectable Chitosan gel (CICGs)	226:266	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
26838881	9	68	theme	fluorescence	1327:1338	arg1	results					1348:1354	In vivo fluorescence imaging results	1319:1354	In vivo fluorescence imaging results	1319:1354	In vivo fluorescence imaging results confirmed that CICGs could stay longer in the joint region when compared to BCGs and MPs.
26838881	10	69	theme	purpose	1494:1500	arg1	CICGs					1462:1466	this novel CICGs	1451:1466	this novel CICGs	1451:1466	Thus this novel CICGs could be a potential dual purpose gel for the treatment of diseased joint regions especially for Osteoarthritis.
26838881	10	69	theme	purpose	1494:1500	arg1	gel					1502:1504	a potential dual purpose gel	1477:1504	a potential dual purpose gel for the treatment of diseased joint regions	1477:1548	Thus this novel CICGs could be a potential dual purpose gel for the treatment of diseased joint regions especially for Osteoarthritis.
26838881	10	70	theme	diseased	1527:1534	arg1	regions					1542:1548	diseased joint regions	1527:1548	diseased joint regions	1527:1548	Thus this novel CICGs could be a potential dual purpose gel for the treatment of diseased joint regions especially for Osteoarthritis.
26838881	1	71	theme	injectable	236:245	arg1	CICGs					261:265	composite injectable Chitosan gel (CICGs)	226:266	composite injectable Chitosan gel (CICGs)	226:266	This study aimed to evaluate Poly (caprolactone) microparticles (MPs) loaded composite injectable Chitosan gel (CICGs) as a dual purpose (visco-supplement and intra articular drug delivery depot) therapeutic agent for the treatment of Osteoarthritis.
28148266	8	0	theme	UCB-MSCs	1244:1251	arg1	hydrogel					1260:1267	HA hydrogel	1257:1267	HA hydrogel	1257:1267	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	8	0	theme	UCB-MSCs	1244:1251	arg1	composite					1220:1228	the composite	1216:1228	the composite of allogeneic UCB-MSCs and HA hydrogel	1216:1267	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	8	0	theme	UCB-MSCs	1244:1251	arg1	UCB-MSCs					1244:1251	allogeneic UCB-MSCs	1233:1251	allogeneic UCB-MSCs	1233:1251	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	0	1	theme	case	161:164	arg1	report					166:171	a case report	159:171	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.	0:196	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	8	2	theme	effective	1298:1306	arg1	option					1318:1323	a safe and effective treatment option	1287:1323	a safe and effective treatment option for large osteochondral defects of the knee	1287:1367	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	1	3	theme	articular	226:234	arg1	defects					246:252	articular cartilage defects	226:252	articular cartilage defects	226:252	BACKGROUND The treatment of articular cartilage defects is a therapeutic challenge for orthopaedic surgeons.
28148266	5	4	theme	normal	914:919	arg1	cartilage					921:929	the surrounding normal cartilage	898:929	the surrounding normal cartilage	898:929	A hyaline-like cartilage completely filled the defect and was congruent with the surrounding normal cartilage as revealed by magnetic resonance imaging (MRI), a second-look arthroscopy and histological assessment.
28148266	7	5	theme	restored	1133:1140	arg1	tissues					1165:1171	the restored bony and cartilaginous tissues	1129:1171	the restored bony and cartilaginous tissues	1129:1171	MRI also showed the maintenance of the restored bony and cartilaginous tissues.
28148266	8	6	theme	osteochondral	1335:1347	arg1	defects					1349:1355	large osteochondral defects	1329:1355	large osteochondral defects of the knee	1329:1367	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	1	7	theme	cartilage	236:244	arg1	defects					246:252	articular cartilage defects	226:252	articular cartilage defects	226:252	BACKGROUND The treatment of articular cartilage defects is a therapeutic challenge for orthopaedic surgeons.
28148266	0	8	theme	umbilical	77:85	arg1	cells					123:127	umbilical cord blood-derived mesenchymal stem cells	77:127	umbilical cord blood-derived mesenchymal stem cells	77:127	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	8	9	dep	CONCLUSION	1174:1183	arg1	suggests					1202:1209	suggests	1202:1209	suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee	1202:1367	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	4	10	theme	joint	753:757	arg1	function					732:739	function	732:739	function	732:739	Significant improvements in pain and function of the knee joint were identified by the evaluation at 12 months after surgery.
28148266	4	10	theme	joint	753:757	arg1	pain					723:726	pain	723:726	pain	723:726	Significant improvements in pain and function of the knee joint were identified by the evaluation at 12 months after surgery.
28148266	1	11	theme	defects	246:252	arg1	treatment					213:221	The treatment	209:221	The treatment of articular cartilage defects	209:252	BACKGROUND The treatment of articular cartilage defects is a therapeutic challenge for orthopaedic surgeons.
28148266	1	11	theme	defects	246:252	arg1	challenge					271:279	a therapeutic challenge	257:279	a therapeutic challenge for orthopaedic surgeons	257:304	BACKGROUND The treatment of articular cartilage defects is a therapeutic challenge for orthopaedic surgeons.
28148266	5	12	with	congruent	883:891	arg1	cartilage					921:929	the surrounding normal cartilage	898:929	the surrounding normal cartilage	898:929	A hyaline-like cartilage completely filled the defect and was congruent with the surrounding normal cartilage as revealed by magnetic resonance imaging (MRI), a second-look arthroscopy and histological assessment.
28148266	7	13	theme	cartilaginous	1151:1163	arg1	tissues					1165:1171	the restored bony and cartilaginous tissues	1129:1171	the restored bony and cartilaginous tissues	1129:1171	MRI also showed the maintenance of the restored bony and cartilaginous tissues.
28148266	4	14	theme	knee	748:751	arg1	joint					753:757	the knee joint	744:757	the knee joint	744:757	Significant improvements in pain and function of the knee joint were identified by the evaluation at 12 months after surgery.
28148266	8	15	theme	treatment	1308:1316	arg1	option					1318:1323	a safe and effective treatment option	1287:1323	a safe and effective treatment option for large osteochondral defects of the knee	1287:1367	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	0	16	theme	blood-derived	92:104	arg1	cells					123:127	umbilical cord blood-derived mesenchymal stem cells	77:127	umbilical cord blood-derived mesenchymal stem cells	77:127	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	5	17	theme	surrounding	902:912	arg1	cartilage					921:929	the surrounding normal cartilage	898:929	the surrounding normal cartilage	898:929	A hyaline-like cartilage completely filled the defect and was congruent with the surrounding normal cartilage as revealed by magnetic resonance imaging (MRI), a second-look arthroscopy and histological assessment.
28148266	0	18	theme	5-year	180:185	arg1	follow-up					187:195	a 5-year follow-up	178:195	a 5-year follow-up	178:195	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	6	19	theme	clinical	1048:1055	arg1	outcomes					1057:1064	The improved clinical outcomes	1035:1064	The improved clinical outcomes	1035:1064	The improved clinical outcomes maintained until 5.5 years.
28148266	0	20	with	report	166:171	arg1	follow-up					187:195	a 5-year follow-up	178:195	a 5-year follow-up	178:195	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	2	21	theme	sufficient	393:402	arg1	characteristics					418:432	sufficient biomechanical characteristics	393:432	sufficient biomechanical characteristics as well as the overlying cartilage	393:467	Furthermore, large osteochondral defects needs restoration of the underlying bone for sufficient biomechanical characteristics as well as the overlying cartilage.
28148266	0	22	theme	cord	87:90	arg1	cells					123:127	umbilical cord blood-derived mesenchymal stem cells	77:127	umbilical cord blood-derived mesenchymal stem cells	77:127	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	3	23	link	blood-derived	596:608	arg1	hydrogel					685:692	4% hyaluronic acid (HA) hydrogel	661:692	4% hyaluronic acid (HA) hydrogel	661:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	23	link	blood-derived	596:608	arg1	UCB-MSCs					634:641	UCB-MSCs	634:641	UCB-MSCs	634:641	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	23	link	blood-derived	596:608	arg1	cells					627:631	umbilical cord blood-derived mesenchymal stem cells	581:631	umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	581:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	23	link	blood-derived	596:608	arg1	107/ml					650:655	0.5 x 107/ml	644:655	0.5 x 107/ml	644:655	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	8	24	theme	knee	1364:1367	arg1	defects					1349:1355	large osteochondral defects	1329:1355	large osteochondral defects of the knee	1329:1367	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	8	25	theme	case	1190:1193	arg1	report					1195:1200	This case report	1185:1200	This case report	1185:1200	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	5	26	theme	histological	1010:1021	arg1	assessment					1023:1032	histological assessment	1010:1032	histological assessment	1010:1032	A hyaline-like cartilage completely filled the defect and was congruent with the surrounding normal cartilage as revealed by magnetic resonance imaging (MRI), a second-look arthroscopy and histological assessment.
28148266	3	27	theme	umbilical	581:589	arg1	hydrogel					685:692	4% hyaluronic acid (HA) hydrogel	661:692	4% hyaluronic acid (HA) hydrogel	661:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	27	theme	umbilical	581:589	arg1	UCB-MSCs					634:641	UCB-MSCs	634:641	UCB-MSCs	634:641	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	27	theme	umbilical	581:589	arg1	cells					627:631	umbilical cord blood-derived mesenchymal stem cells	581:631	umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	581:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	27	theme	umbilical	581:589	arg1	107/ml					650:655	0.5 x 107/ml	644:655	0.5 x 107/ml	644:655	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	2	28	theme	bone	384:387	arg1	restoration					354:364	restoration	354:364	restoration of the underlying bone for sufficient biomechanical characteristics as well as the overlying cartilage	354:467	Furthermore, large osteochondral defects needs restoration of the underlying bone for sufficient biomechanical characteristics as well as the overlying cartilage.
28148266	2	29	theme	overlying	449:457	arg1	cartilage					459:467	the overlying cartilage	445:467	sufficient biomechanical characteristics as well as the overlying cartilage	393:467	Furthermore, large osteochondral defects needs restoration of the underlying bone for sufficient biomechanical characteristics as well as the overlying cartilage.
28148266	7	30	theme	tissues	1165:1171	arg1	maintenance					1114:1124	the maintenance	1110:1124	the maintenance of the restored bony and cartilaginous tissues	1110:1171	MRI also showed the maintenance of the restored bony and cartilaginous tissues.
28148266	3	31	theme	hyaluronic	664:673	arg1	HA					681:682	HA	681:682	HA	681:682	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	31	theme	hyaluronic	664:673	arg1	acid					675:678	hyaluronic acid	664:678	4% hyaluronic acid (HA) hydrogel	661:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	32	theme	cord	591:594	arg1	hydrogel					685:692	4% hyaluronic acid (HA) hydrogel	661:692	4% hyaluronic acid (HA) hydrogel	661:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	32	theme	cord	591:594	arg1	UCB-MSCs					634:641	UCB-MSCs	634:641	UCB-MSCs	634:641	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	32	theme	cord	591:594	arg1	cells					627:631	umbilical cord blood-derived mesenchymal stem cells	581:631	umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	581:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	32	theme	cord	591:594	arg1	107/ml					650:655	0.5 x 107/ml	644:655	0.5 x 107/ml	644:655	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	2	33	theme	underlying	373:382	arg1	bone					384:387	the underlying bone	369:387	the underlying bone	369:387	Furthermore, large osteochondral defects needs restoration of the underlying bone for sufficient biomechanical characteristics as well as the overlying cartilage.
28148266	0	34	theme	large	17:21	arg1	defect					37:42	a large osteochondral defect	15:42	a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel	15:156	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	1	35	theme	therapeutic	259:269	arg1	challenge					271:279	a therapeutic challenge	257:279	a therapeutic challenge for orthopaedic surgeons	257:304	BACKGROUND The treatment of articular cartilage defects is a therapeutic challenge for orthopaedic surgeons.
28148266	1	35	theme	therapeutic	259:269	arg1	treatment					213:221	The treatment	209:221	The treatment of articular cartilage defects	209:252	BACKGROUND The treatment of articular cartilage defects is a therapeutic challenge for orthopaedic surgeons.
28148266	0	36	theme	stem	118:121	arg1	cells					123:127	umbilical cord blood-derived mesenchymal stem cells	77:127	umbilical cord blood-derived mesenchymal stem cells	77:127	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	3	37	from	defect	522:527	arg1	joint					541:545	the knee joint	532:545	the knee joint	532:545	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	5	38	theme	magnetic	946:953	arg1	MRI					974:976	MRI	974:976	MRI	974:976	A hyaline-like cartilage completely filled the defect and was congruent with the surrounding normal cartilage as revealed by magnetic resonance imaging (MRI), a second-look arthroscopy and histological assessment.
28148266	5	38	theme	magnetic	946:953	arg1	imaging					965:971	magnetic resonance imaging	946:971	magnetic resonance imaging (MRI)	946:977	A hyaline-like cartilage completely filled the defect and was congruent with the surrounding normal cartilage as revealed by magnetic resonance imaging (MRI), a second-look arthroscopy and histological assessment.
28148266	8	39	theme	hydrogel	1260:1267	arg1	hydrogel					1260:1267	HA hydrogel	1257:1267	HA hydrogel	1257:1267	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	8	39	theme	hydrogel	1260:1267	arg1	composite					1220:1228	the composite	1216:1228	the composite of allogeneic UCB-MSCs and HA hydrogel	1216:1267	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	8	39	theme	hydrogel	1260:1267	arg1	UCB-MSCs					1244:1251	allogeneic UCB-MSCs	1233:1251	allogeneic UCB-MSCs	1233:1251	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	0	40	theme	mesenchymal	106:116	arg1	cells					123:127	umbilical cord blood-derived mesenchymal stem cells	77:127	umbilical cord blood-derived mesenchymal stem cells	77:127	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	5	41	theme	resonance	955:963	arg1	MRI					974:976	MRI	974:976	MRI	974:976	A hyaline-like cartilage completely filled the defect and was congruent with the surrounding normal cartilage as revealed by magnetic resonance imaging (MRI), a second-look arthroscopy and histological assessment.
28148266	5	41	theme	resonance	955:963	arg1	imaging					965:971	magnetic resonance imaging	946:971	magnetic resonance imaging (MRI)	946:977	A hyaline-like cartilage completely filled the defect and was congruent with the surrounding normal cartilage as revealed by magnetic resonance imaging (MRI), a second-look arthroscopy and histological assessment.
28148266	3	42	theme	x	648:648	arg1	cells					627:631	umbilical cord blood-derived mesenchymal stem cells	581:631	umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	581:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	42	theme	x	648:648	arg1	107/ml					650:655	0.5 x 107/ml	644:655	0.5 x 107/ml	644:655	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	5	43	theme	hyaline-like	823:834	arg1	cartilage					836:844	A hyaline-like cartilage	821:844	A hyaline-like cartilage	821:844	A hyaline-like cartilage completely filled the defect and was congruent with the surrounding normal cartilage as revealed by magnetic resonance imaging (MRI), a second-look arthroscopy and histological assessment.
28148266	5	43	theme	hyaline-like	823:834	arg1	congruent					883:891	congruent	883:891	congruent	883:891	A hyaline-like cartilage completely filled the defect and was congruent with the surrounding normal cartilage as revealed by magnetic resonance imaging (MRI), a second-look arthroscopy and histological assessment.
28148266	0	44	theme	defect	37:42	arg1	Restoration					0:10	Restoration	0:10	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.	0:196	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	3	45	dep	restored	551:558	arg1	PRESENTATION					475:486	CASE PRESENTATION	470:486	CASE PRESENTATION	470:486	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	46	theme	4	661:661	arg1	%					662:662	%	662:662	%	662:662	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	47	theme	stem	622:625	arg1	hydrogel					685:692	4% hyaluronic acid (HA) hydrogel	661:692	4% hyaluronic acid (HA) hydrogel	661:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	47	theme	stem	622:625	arg1	UCB-MSCs					634:641	UCB-MSCs	634:641	UCB-MSCs	634:641	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	47	theme	stem	622:625	arg1	cells					627:631	umbilical cord blood-derived mesenchymal stem cells	581:631	umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	581:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	47	theme	stem	622:625	arg1	107/ml					650:655	0.5 x 107/ml	644:655	0.5 x 107/ml	644:655	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	48	dep	cells	627:631	arg1	hydrogel					685:692	4% hyaluronic acid (HA) hydrogel	661:692	4% hyaluronic acid (HA) hydrogel	661:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	48	dep	cells	627:631	arg1	cells					627:631	umbilical cord blood-derived mesenchymal stem cells	581:631	umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	581:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	48	dep	cells	627:631	arg1	107/ml					650:655	0.5 x 107/ml	644:655	0.5 x 107/ml	644:655	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	6	49	theme	improved	1039:1046	arg1	outcomes					1057:1064	The improved clinical outcomes	1035:1064	The improved clinical outcomes	1035:1064	The improved clinical outcomes maintained until 5.5 years.
28148266	0	50	theme	osteochondral	23:35	arg1	defect					37:42	a large osteochondral defect	15:42	a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel	15:156	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	1	51	theme	orthopaedic	285:295	arg1	surgeons					297:304	orthopaedic surgeons	285:304	orthopaedic surgeons	285:304	BACKGROUND The treatment of articular cartilage defects is a therapeutic challenge for orthopaedic surgeons.
28148266	0	52	theme	cells	123:127	arg1	cells					123:127	umbilical cord blood-derived mesenchymal stem cells	77:127	umbilical cord blood-derived mesenchymal stem cells	77:127	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	0	52	theme	cells	123:127	arg1	composite					64:72	a composite	62:72	a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel	62:156	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	0	52	theme	cells	123:127	arg1	hydrogel					149:156	hyaluronic acid hydrogel	133:156	hyaluronic acid hydrogel	133:156	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	1	53	dep	BACKGROUND	198:207	arg1	challenge					271:279	a therapeutic challenge	257:279	a therapeutic challenge for orthopaedic surgeons	257:304	BACKGROUND The treatment of articular cartilage defects is a therapeutic challenge for orthopaedic surgeons.
28148266	1	53	dep	BACKGROUND	198:207	arg1	treatment					213:221	The treatment	209:221	The treatment of articular cartilage defects	209:252	BACKGROUND The treatment of articular cartilage defects is a therapeutic challenge for orthopaedic surgeons.
28148266	3	54	theme	cells	627:631	arg1	cells					627:631	umbilical cord blood-derived mesenchymal stem cells	581:631	umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	581:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	54	theme	cells	627:631	arg1	composite					568:576	a composite	566:576	a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	566:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	2	55	theme	biomechanical	404:416	arg1	characteristics					418:432	sufficient biomechanical characteristics	393:432	sufficient biomechanical characteristics as well as the overlying cartilage	393:467	Furthermore, large osteochondral defects needs restoration of the underlying bone for sufficient biomechanical characteristics as well as the overlying cartilage.
28148266	7	56	theme	bony	1142:1145	arg1	tissues					1165:1171	the restored bony and cartilaginous tissues	1129:1171	the restored bony and cartilaginous tissues	1129:1171	MRI also showed the maintenance of the restored bony and cartilaginous tissues.
28148266	0	57	theme	acid	144:147	arg1	hydrogel					149:156	hyaluronic acid hydrogel	133:156	hyaluronic acid hydrogel	133:156	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	3	58	theme	blood-derived	596:608	arg1	hydrogel					685:692	4% hyaluronic acid (HA) hydrogel	661:692	4% hyaluronic acid (HA) hydrogel	661:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	58	theme	blood-derived	596:608	arg1	UCB-MSCs					634:641	UCB-MSCs	634:641	UCB-MSCs	634:641	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	58	theme	blood-derived	596:608	arg1	cells					627:631	umbilical cord blood-derived mesenchymal stem cells	581:631	umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	581:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	58	theme	blood-derived	596:608	arg1	107/ml					650:655	0.5 x 107/ml	644:655	0.5 x 107/ml	644:655	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	59	theme	osteochondral	508:520	arg1	defect					522:527	A symptomatic large osteochondral defect	488:527	A symptomatic large osteochondral defect in the knee joint	488:545	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	8	60	theme	allogeneic	1233:1242	arg1	UCB-MSCs					1244:1251	allogeneic UCB-MSCs	1233:1251	allogeneic UCB-MSCs	1233:1251	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	0	61	theme	hyaluronic	133:142	arg1	hydrogel					149:156	hyaluronic acid hydrogel	133:156	hyaluronic acid hydrogel	133:156	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	3	62	theme	acid	675:678	arg1	hydrogel					685:692	4% hyaluronic acid (HA) hydrogel	661:692	4% hyaluronic acid (HA) hydrogel	661:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	62	theme	acid	675:678	arg1	cells					627:631	umbilical cord blood-derived mesenchymal stem cells	581:631	umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	581:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	63	theme	mesenchymal	610:620	arg1	hydrogel					685:692	4% hyaluronic acid (HA) hydrogel	661:692	4% hyaluronic acid (HA) hydrogel	661:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	63	theme	mesenchymal	610:620	arg1	UCB-MSCs					634:641	UCB-MSCs	634:641	UCB-MSCs	634:641	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	63	theme	mesenchymal	610:620	arg1	cells					627:631	umbilical cord blood-derived mesenchymal stem cells	581:631	umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	581:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	63	theme	mesenchymal	610:620	arg1	107/ml					650:655	0.5 x 107/ml	644:655	0.5 x 107/ml	644:655	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	2	64	theme	osteochondral	326:338	arg1	defects					340:346	large osteochondral defects	320:346	large osteochondral defects	320:346	Furthermore, large osteochondral defects needs restoration of the underlying bone for sufficient biomechanical characteristics as well as the overlying cartilage.
28148266	8	65	theme	safe	1289:1292	arg1	option					1318:1323	a safe and effective treatment option	1287:1323	a safe and effective treatment option for large osteochondral defects of the knee	1287:1367	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	4	66	from	12 months	796:804	arg1	evaluation					782:791	the evaluation	778:791	the evaluation at 12 months after surgery	778:818	Significant improvements in pain and function of the knee joint were identified by the evaluation at 12 months after surgery.
28148266	2	67	theme	large	320:324	arg1	defects					340:346	large osteochondral defects	320:346	large osteochondral defects	320:346	Furthermore, large osteochondral defects needs restoration of the underlying bone for sufficient biomechanical characteristics as well as the overlying cartilage.
28148266	0	68	theme	hydrogel	149:156	arg1	cells					123:127	umbilical cord blood-derived mesenchymal stem cells	77:127	umbilical cord blood-derived mesenchymal stem cells	77:127	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	0	68	theme	hydrogel	149:156	arg1	composite					64:72	a composite	62:72	a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel	62:156	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	0	68	theme	hydrogel	149:156	arg1	hydrogel					149:156	hyaluronic acid hydrogel	133:156	hyaluronic acid hydrogel	133:156	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	3	69	theme	%	662:662	arg1	hydrogel					685:692	4% hyaluronic acid (HA) hydrogel	661:692	4% hyaluronic acid (HA) hydrogel	661:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	69	theme	%	662:662	arg1	cells					627:631	umbilical cord blood-derived mesenchymal stem cells	581:631	umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel	581:692	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	8	70	theme	large	1329:1333	arg1	defects					1349:1355	large osteochondral defects	1329:1355	large osteochondral defects of the knee	1329:1367	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	0	71	link	blood-derived	92:104	arg1	cells					123:127	umbilical cord blood-derived mesenchymal stem cells	77:127	umbilical cord blood-derived mesenchymal stem cells	77:127	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	3	72	theme	large	502:506	arg1	defect					522:527	A symptomatic large osteochondral defect	488:527	A symptomatic large osteochondral defect in the knee joint	488:545	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	4	73	from	improvements	707:718	arg1	function					732:739	function	732:739	function	732:739	Significant improvements in pain and function of the knee joint were identified by the evaluation at 12 months after surgery.
28148266	4	73	from	improvements	707:718	arg1	pain					723:726	pain	723:726	pain	723:726	Significant improvements in pain and function of the knee joint were identified by the evaluation at 12 months after surgery.
28148266	3	74	theme	symptomatic	490:500	arg1	defect					522:527	A symptomatic large osteochondral defect	488:527	A symptomatic large osteochondral defect in the knee joint	488:545	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	3	75	theme	CASE	470:473	arg1	PRESENTATION					475:486	CASE PRESENTATION	470:486	CASE PRESENTATION	470:486	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	5	76	theme	second-look	982:992	arg1	arthroscopy					994:1004	a second-look arthroscopy	980:1004	a second-look arthroscopy	980:1004	A hyaline-like cartilage completely filled the defect and was congruent with the surrounding normal cartilage as revealed by magnetic resonance imaging (MRI), a second-look arthroscopy and histological assessment.
28148266	3	77	theme	knee	536:539	arg1	joint					541:545	the knee joint	532:545	the knee joint	532:545	CASE PRESENTATION A symptomatic large osteochondral defect in the knee joint was restored using a composite of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) 0.5 x 107/ml and 4% hyaluronic acid (HA) hydrogel.
28148266	0	78	theme	knee	51:54	arg1	defect					37:42	a large osteochondral defect	15:42	a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel	15:156	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	0	79	dep	Restoration	0:10	arg1	report					166:171	a case report	159:171	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.	0:196	Restoration of a large osteochondral defect of the knee using a composite of umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel: a case report with a 5-year follow-up.
28148266	8	80	theme	HA	1257:1258	arg1	hydrogel					1260:1267	HA hydrogel	1257:1267	HA hydrogel	1257:1267	CONCLUSION This case report suggests that the composite of allogeneic UCB-MSCs and HA hydrogel can be considered a safe and effective treatment option for large osteochondral defects of the knee.
28148266	4	81	theme	Significant	695:705	arg1	improvements					707:718	Significant improvements	695:718	Significant improvements in pain and function of the knee joint	695:757	Significant improvements in pain and function of the knee joint were identified by the evaluation at 12 months after surgery.
28183630	9	0	theme	drug	1336:1339	arg1	conjugation					1317:1327	conjugation	1317:1327	conjugation of the drug to the polymer backbone	1317:1363	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	9	0	theme	drug	1336:1339	arg1	way					1381:1383	an effective way	1368:1383	an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF	1368:1470	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	7	1	theme	reduced	936:942	arg1	diameter					950:957	a reduced fibre diameter	934:957	a reduced fibre diameter	934:957	Scanning electron microscopy (SEM) showed that the blended CS-PVA nanofibres had a reduced fibre diameter compared to the controls.
28183630	1	2	theme	drug	190:193	arg1	range					114:118	A range	112:118	A range of biodegradable drug-nanofibres composite mats	112:166	A range of biodegradable drug-nanofibres composite mats have been reported as drug delivery systems.
28183630	1	2	theme	drug	190:193	arg1	systems					204:210	drug delivery systems	190:210	drug delivery systems	190:210	A range of biodegradable drug-nanofibres composite mats have been reported as drug delivery systems.
28183630	6	3	theme	1H	845:846	arg1	NMR					848:850	1H NMR	845:850	1H NMR	845:850	The conjugated CS-LVF was characterized by FTIR, DSC, TGA and 1H NMR.
28183630	3	4	theme	drug	378:381	arg1	conjugated-chitosan					383:401	drug conjugated-chitosan	378:401	drug conjugated-chitosan	378:401	This paper reports an improved system based on the incorporation of drug conjugated-chitosan into polyvinyl alcohol (PVA) nanofibers.
28183630	8	5	theme	burst	1019:1023	arg1	release					1025:1031	burst release	1019:1031	burst release	1019:1031	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	7	6	theme	fibre	944:948	arg1	diameter					950:957	a reduced fibre diameter	934:957	a reduced fibre diameter	934:957	Scanning electron microscopy (SEM) showed that the blended CS-PVA nanofibres had a reduced fibre diameter compared to the controls.
28183630	9	7	theme	drug	1462:1465	arg1	LVF					1468:1470	LVF	1468:1470	LVF	1468:1470	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	9	7	theme	drug	1462:1465	arg1	release					1447:1453	sustained release	1437:1453	sustained release of the drug	1437:1465	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	7	8	theme	electron	862:869	arg1	microscopy					871:880	Scanning electron microscopy	853:880	Scanning electron microscopy (SEM)	853:886	Scanning electron microscopy (SEM) showed that the blended CS-PVA nanofibres had a reduced fibre diameter compared to the controls.
28183630	7	8	theme	electron	862:869	arg1	SEM					883:885	SEM	883:885	SEM	883:885	Scanning electron microscopy (SEM) showed that the blended CS-PVA nanofibres had a reduced fibre diameter compared to the controls.
28183630	8	9	theme	PVA/conjugated	1110:1123	arg1	mats					1132:1135	the PVA/conjugated CS-LVF mats	1106:1135	the PVA/conjugated CS-LVF mats	1106:1135	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	3	10	theme	conjugated-chitosan	383:401	arg1	incorporation					361:373	the incorporation	357:373	the incorporation of drug conjugated-chitosan into polyvinyl alcohol (PVA) nanofibers	357:441	This paper reports an improved system based on the incorporation of drug conjugated-chitosan into polyvinyl alcohol (PVA) nanofibers.
28183630	9	11	theme	polymer	1348:1354	arg1	backbone					1356:1363	the polymer backbone	1344:1363	the polymer backbone	1344:1363	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	5	12	theme	PVA-CS/LVF	732:741	arg1	nanofibres					743:752	PVA/LVF and PVA-CS/LVF nanofibres	720:752	PVA/LVF and PVA-CS/LVF nanofibres	720:752	PVA/LVF and PVA-CS/LVF nanofibres were fabricated as controls.
28183630	5	12	theme	PVA-CS/LVF	732:741	arg1	controls					773:780	controls	773:780	controls	773:780	PVA/LVF and PVA-CS/LVF nanofibres were fabricated as controls.
28183630	8	13	from	37°C	1169:1172	arg1	buffer					1159:1164	phosphate buffer	1149:1164	phosphate buffer at 37°C	1149:1172	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	7	14	theme	Scanning	853:860	arg1	microscopy					871:880	Scanning electron microscopy	853:880	Scanning electron microscopy (SEM)	853:886	Scanning electron microscopy (SEM) showed that the blended CS-PVA nanofibres had a reduced fibre diameter compared to the controls.
28183630	7	14	theme	Scanning	853:860	arg1	SEM					883:885	SEM	883:885	SEM	883:885	Scanning electron microscopy (SEM) showed that the blended CS-PVA nanofibres had a reduced fibre diameter compared to the controls.
28183630	1	15	theme	delivery	195:202	arg1	range					114:118	A range	112:118	A range of biodegradable drug-nanofibres composite mats	112:166	A range of biodegradable drug-nanofibres composite mats have been reported as drug delivery systems.
28183630	1	15	theme	delivery	195:202	arg1	systems					204:210	drug delivery systems	190:210	drug delivery systems	190:210	A range of biodegradable drug-nanofibres composite mats have been reported as drug delivery systems.
28183630	8	16	theme	CS-LVF	1125:1130	arg1	mats					1132:1135	the PVA/conjugated CS-LVF mats	1106:1135	the PVA/conjugated CS-LVF mats	1106:1135	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	9	17	dep	pH.	1287:1289	arg1	indicate					1303:1310	indicate	1303:1310	indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF	1303:1470	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	7	18	theme	CS-PVA	912:917	arg1	nanofibres					919:928	the blended CS-PVA nanofibres	900:928	the blended CS-PVA nanofibres	900:928	Scanning electron microscopy (SEM) showed that the blended CS-PVA nanofibres had a reduced fibre diameter compared to the controls.
28183630	0	19	theme	alcohol	10:16	arg1	nanofibres					28:37	Polyvinyl alcohol composite nanofibres	0:37	Polyvinyl alcohol composite nanofibres	0:37	Polyvinyl alcohol composite nanofibres containing conjugated levofloxacin-chitosan for controlled drug release.
28183630	6	20	theme	conjugated	787:796	arg1	CS-LVF					798:803	The conjugated CS-LVF	783:803	The conjugated CS-LVF	783:803	The conjugated CS-LVF was characterized by FTIR, DSC, TGA and 1H NMR.
28183630	8	21	theme	control	1063:1069	arg1	mats					1091:1094	the control PVA/LVF electrospun mats	1059:1094	the control PVA/LVF electrospun mats	1059:1094	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	4	22	theme	alcohol	669:675	arg1	nanofibres					683:692	polyvinyl alcohol (PVA) nanofibres	659:692	polyvinyl alcohol (PVA) nanofibres	659:692	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	0	23	theme	Polyvinyl	0:8	arg1	nanofibres					28:37	Polyvinyl alcohol composite nanofibres	0:37	Polyvinyl alcohol composite nanofibres	0:37	Polyvinyl alcohol composite nanofibres containing conjugated levofloxacin-chitosan for controlled drug release.
28183630	4	24	theme	weight	570:575	arg1	CS					587:588	CS	587:588	CS	587:588	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	4	24	theme	weight	570:575	arg1	chitosan					577:584	low molecular weight chitosan	556:584	low molecular weight chitosan (CS) via a cleavable amide bond	556:616	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	8	25	theme	release	990:996	arg1	profiles					998:1005	Drug release profiles	985:1005	Drug release profiles	985:1005	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	4	26	theme	polyvinyl	659:667	arg1	PVA					678:680	PVA	678:680	PVA	678:680	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	4	26	theme	polyvinyl	659:667	arg1	alcohol					669:675	polyvinyl alcohol	659:675	polyvinyl alcohol (PVA) nanofibres	659:692	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	8	27	theme	phosphate	1149:1157	arg1	buffer					1159:1164	phosphate buffer	1149:1164	phosphate buffer at 37°C	1149:1172	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	4	28	theme	molecular	560:568	arg1	weight					570:575	low molecular weight	556:575	low molecular weight chitosan (CS) via a cleavable amide bond	556:616	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	7	29	theme	blended	904:910	arg1	nanofibres					919:928	the blended CS-PVA nanofibres	900:928	the blended CS-PVA nanofibres	900:928	Scanning electron microscopy (SEM) showed that the blended CS-PVA nanofibres had a reduced fibre diameter compared to the controls.
28183630	9	30	theme	slower	1180:1185	arg1	release					1187:1193	This slower release	1175:1193	This slower release	1175:1193	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	3	31	theme	polyvinyl	408:416	arg1	alcohol					418:424	polyvinyl alcohol	408:424	polyvinyl alcohol (PVA) nanofibers	408:441	This paper reports an improved system based on the incorporation of drug conjugated-chitosan into polyvinyl alcohol (PVA) nanofibers.
28183630	3	31	theme	polyvinyl	408:416	arg1	PVA					427:429	PVA	427:429	PVA	427:429	This paper reports an improved system based on the incorporation of drug conjugated-chitosan into polyvinyl alcohol (PVA) nanofibers.
28183630	1	32	theme	biodegradable	123:135	arg1	mats					163:166	biodegradable drug-nanofibres composite mats	123:166	biodegradable drug-nanofibres composite mats	123:166	A range of biodegradable drug-nanofibres composite mats have been reported as drug delivery systems.
28183630	0	33	theme	conjugated	50:59	arg1	levofloxacin-chitosan					61:81	conjugated levofloxacin-chitosan	50:81	conjugated levofloxacin-chitosan for controlled drug release	50:109	Polyvinyl alcohol composite nanofibres containing conjugated levofloxacin-chitosan for controlled drug release.
28183630	4	34	theme	controlled	468:477	arg1	release					479:485	controlled release	468:485	controlled release of levofloxacin (LVF)	468:507	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	9	35	theme	effective	1371:1379	arg1	conjugation					1317:1327	conjugation	1317:1327	conjugation of the drug to the polymer backbone	1317:1363	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	9	35	theme	effective	1371:1379	arg1	way					1381:1383	an effective way	1368:1383	an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF	1368:1470	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	4	36	theme	conjugated	640:649	arg1	CS					651:652	the conjugated CS	636:652	the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning	636:717	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	7	37	contain	had	930:932	arg2	diameter					950:957	a reduced fibre diameter	934:957	a reduced fibre diameter	934:957	Scanning electron microscopy (SEM) showed that the blended CS-PVA nanofibres had a reduced fibre diameter compared to the controls.
28183630	7	37	contain	had	930:932	arg1	nanofibres					919:928	the blended CS-PVA nanofibres	900:928	the blended CS-PVA nanofibres	900:928	Scanning electron microscopy (SEM) showed that the blended CS-PVA nanofibres had a reduced fibre diameter compared to the controls.
28183630	4	38	theme	levofloxacin	490:501	arg1	release					479:485	controlled release	468:485	controlled release of levofloxacin (LVF)	468:507	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	2	39	theme	rapid	253:257	arg1	release					259:265	the rapid release	249:265	the rapid release of the drug	249:277	However, their main disadvantage is the rapid release of the drug immediately after application.
28183630	2	39	theme	rapid	253:257	arg1	disadvantage					233:244	their main disadvantage	222:244	their main disadvantage	222:244	However, their main disadvantage is the rapid release of the drug immediately after application.
28183630	9	40	theme	burst	1399:1403	arg1	behaviour					1413:1421	burst release behaviour	1399:1421	burst release behaviour	1399:1421	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	5	41	theme	PVA/LVF	720:726	arg1	nanofibres					743:752	PVA/LVF and PVA-CS/LVF nanofibres	720:752	PVA/LVF and PVA-CS/LVF nanofibres	720:752	PVA/LVF and PVA-CS/LVF nanofibres were fabricated as controls.
28183630	5	41	theme	PVA/LVF	720:726	arg1	controls					773:780	controls	773:780	controls	773:780	PVA/LVF and PVA-CS/LVF nanofibres were fabricated as controls.
28183630	8	42	theme	PVA/LVF	1071:1077	arg1	mats					1091:1094	the control PVA/LVF electrospun mats	1059:1094	the control PVA/LVF electrospun mats	1059:1094	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	4	43	with	CS	651:652	arg1	nanofibres					683:692	polyvinyl alcohol (PVA) nanofibres	659:692	polyvinyl alcohol (PVA) nanofibres	659:692	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	1	44	theme	drug-nanofibres	137:151	arg1	mats					163:166	biodegradable drug-nanofibres composite mats	123:166	biodegradable drug-nanofibres composite mats	123:166	A range of biodegradable drug-nanofibres composite mats have been reported as drug delivery systems.
28183630	4	45	theme	low	556:558	arg1	weight					570:575	low molecular weight	556:575	low molecular weight chitosan (CS) via a cleavable amide bond	556:616	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	3	46	theme	improved	332:339	arg1	system					341:346	an improved system	329:346	an improved system based on the incorporation of drug conjugated-chitosan into polyvinyl alcohol (PVA) nanofibers	329:441	This paper reports an improved system based on the incorporation of drug conjugated-chitosan into polyvinyl alcohol (PVA) nanofibers.
28183630	9	47	theme	release	1405:1411	arg1	behaviour					1413:1421	burst release behaviour	1399:1421	burst release behaviour	1399:1421	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	1	48	theme	composite	153:161	arg1	mats					163:166	biodegradable drug-nanofibres composite mats	123:166	biodegradable drug-nanofibres composite mats	123:166	A range of biodegradable drug-nanofibres composite mats have been reported as drug delivery systems.
28183630	8	49	from	%	1101:1101	arg1	mats					1132:1135	the PVA/conjugated CS-LVF mats	1106:1135	the PVA/conjugated CS-LVF mats	1106:1135	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	8	50	theme	Drug	985:988	arg1	profiles					998:1005	Drug release profiles	985:1005	Drug release profiles	985:1005	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	2	51	theme	main	228:231	arg1	release					259:265	the rapid release	249:265	the rapid release of the drug	249:277	However, their main disadvantage is the rapid release of the drug immediately after application.
28183630	2	51	theme	main	228:231	arg1	disadvantage					233:244	their main disadvantage	222:244	their main disadvantage	222:244	However, their main disadvantage is the rapid release of the drug immediately after application.
28183630	1	52	theme	mats	163:166	arg1	range					114:118	A range	112:118	A range of biodegradable drug-nanofibres composite mats	112:166	A range of biodegradable drug-nanofibres composite mats have been reported as drug delivery systems.
28183630	1	52	theme	mats	163:166	arg1	systems					204:210	drug delivery systems	190:210	drug delivery systems	190:210	A range of biodegradable drug-nanofibres composite mats have been reported as drug delivery systems.
28183630	4	53	theme	amide	607:611	arg1	bond					613:616	a cleavable amide bond	595:616	a cleavable amide bond	595:616	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	8	54	theme	electrospun	1079:1089	arg1	mats					1091:1094	the control PVA/LVF electrospun mats	1059:1094	the control PVA/LVF electrospun mats	1059:1094	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	2	55	theme	drug	274:277	arg1	release					259:265	the rapid release	249:265	the rapid release of the drug	249:277	However, their main disadvantage is the rapid release of the drug immediately after application.
28183630	2	55	theme	drug	274:277	arg1	disadvantage					233:244	their main disadvantage	222:244	their main disadvantage	222:244	However, their main disadvantage is the rapid release of the drug immediately after application.
28183630	4	56	theme	cleavable	597:605	arg1	bond					613:616	a cleavable amide bond	595:616	a cleavable amide bond	595:616	The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning.
28183630	0	57	theme	drug	98:101	arg1	release					103:109	controlled drug release	87:109	controlled drug release	87:109	Polyvinyl alcohol composite nanofibres containing conjugated levofloxacin-chitosan for controlled drug release.
28183630	9	58	theme	cleavable	1209:1217	arg1	bond					1219:1222	the cleavable bond	1205:1222	the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF	1205:1470	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	9	59	theme	sustained	1437:1445	arg1	LVF					1468:1470	LVF	1468:1470	LVF	1468:1470	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	9	59	theme	sustained	1437:1445	arg1	release					1447:1453	sustained release	1437:1453	sustained release of the drug	1437:1465	This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
28183630	8	60	from	%	1054:1054	arg1	mats					1091:1094	the control PVA/LVF electrospun mats	1059:1094	the control PVA/LVF electrospun mats	1059:1094	Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C.
28183630	0	61	theme	controlled	87:96	arg1	release					103:109	controlled drug release	87:109	controlled drug release	87:109	Polyvinyl alcohol composite nanofibres containing conjugated levofloxacin-chitosan for controlled drug release.
28183630	0	62	theme	composite	18:26	arg1	nanofibres					28:37	Polyvinyl alcohol composite nanofibres	0:37	Polyvinyl alcohol composite nanofibres	0:37	Polyvinyl alcohol composite nanofibres containing conjugated levofloxacin-chitosan for controlled drug release.
28183630	3	63	theme	alcohol	418:424	arg1	nanofibers					432:441	polyvinyl alcohol (PVA) nanofibers	408:441	polyvinyl alcohol (PVA) nanofibers	408:441	This paper reports an improved system based on the incorporation of drug conjugated-chitosan into polyvinyl alcohol (PVA) nanofibers.
26598045	9	0	theme	g	1505:1505	arg1	extract					1511:1517	0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract	1448:1517	0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract	1448:1517	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	7	1	theme	essential	1054:1062	arg1	acids					1070:1074	all essential amino acids	1050:1074	all essential amino acids	1050:1074	The amino acid composition of the protein contains all essential amino acids, with a protein score of 47.
26598045	11	2	theme	E	1675:1675	arg1	sclerotium					1677:1686	L. tigris E sclerotium	1665:1686	L. tigris E sclerotium	1665:1686	In conclusion, L. tigris E sclerotium has the potential to be developed into a functional food and nutraceutical.
26598045	10	3	theme	L.	1575:1576	arg1	powder					1598:1603	L. tigris E sclerotial powder	1575:1603	L. tigris E sclerotial powder	1575:1603	Acute toxicity assessment indicated that L. tigris E sclerotial powder was not toxic at the dose of 2000 mg kg(-1).
26598045	4	4	theme	antioxidant	603:613	arg1	activities					615:624	antioxidant activities	603:624	antioxidant activities of the sclerotial extracts	603:651	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	10	5	theme	sclerotial	1587:1596	arg1	powder					1598:1603	L. tigris E sclerotial powder	1575:1603	L. tigris E sclerotial powder	1575:1603	Acute toxicity assessment indicated that L. tigris E sclerotial powder was not toxic at the dose of 2000 mg kg(-1).
26598045	8	6	theme	sclerotial	1109:1118	arg1	extracts					1120:1127	The sclerotial extracts	1105:1127	The sclerotial extracts	1105:1127	The sclerotial extracts contain phenolics, terpenoids, and glucan.
26598045	2	7	theme	important	190:198	arg1	mushroom					210:217	an important medicinal mushroom	187:217	an important medicinal mushroom	187:217	is an important medicinal mushroom in Southeast Asia and has been consumed frequently by the natives as a cure for a variety of illnesses.
26598045	4	8	theme	sclerotium	573:582	arg1	chemical					529:536	chemical	529:536	chemical	529:536	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	7	9	theme	amino	1003:1007	arg1	composition					1014:1024	The amino acid composition	999:1024	The amino acid composition of the protein	999:1039	The amino acid composition of the protein contains all essential amino acids, with a protein score of 47.
26598045	5	10	theme	tigris	812:817	arg1	sclerotium					821:830	the L. tigris E sclerotium	805:830	the L. tigris E sclerotium	805:830	Acute toxicity of the L. tigris E sclerotium was assessed using a rat model study.
26598045	9	11	dep	9.613	1475:1479	arg1	to					1472:1473	to	1472:1473	to	1472:1473	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	0	12	theme	cultivar	132:139	arg1	E					141:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	10	13	theme	mg	1639:1640	arg1	-1					1645:1646	-1	1645:1646	-1	1645:1646	Acute toxicity assessment indicated that L. tigris E sclerotial powder was not toxic at the dose of 2000 mg kg(-1).
26598045	10	13	theme	mg	1639:1640	arg1	kg					1642:1643	2000 mg kg	1634:1643	2000 mg kg(-1)	1634:1647	Acute toxicity assessment indicated that L. tigris E sclerotial powder was not toxic at the dose of 2000 mg kg(-1).
26598045	5	14	theme	sclerotium	821:830	arg1	toxicity					793:800	Acute toxicity	787:800	Acute toxicity of the L. tigris E sclerotium	787:830	Acute toxicity of the L. tigris E sclerotium was assessed using a rat model study.
26598045	3	15	contain	contain	403:409	arg2	value					428:432	high nutritional value	411:432	high nutritional value	411:432	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	3	15	contain	contain	403:409	arg1	sclerotium					388:397	Lignosus tigris (cultivar E) sclerotium	359:397	Lignosus tigris (cultivar E) sclerotium	359:397	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	3	15	contain	contain	403:409	arg2	properties					450:459	antioxidant properties	438:459	antioxidant properties	438:459	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	8	16	contain	contain	1129:1135	arg2	terpenoids					1148:1157	terpenoids	1148:1157	terpenoids	1148:1157	The sclerotial extracts contain phenolics, terpenoids, and glucan.
26598045	8	16	contain	contain	1129:1135	arg2	phenolics					1137:1145	phenolics	1137:1145	phenolics	1137:1145	The sclerotial extracts contain phenolics, terpenoids, and glucan.
26598045	8	16	contain	contain	1129:1135	arg2	glucan					1164:1169	glucan	1164:1169	glucan	1164:1169	The sclerotial extracts contain phenolics, terpenoids, and glucan.
26598045	8	16	contain	contain	1129:1135	arg1	extracts					1120:1127	The sclerotial extracts	1105:1127	The sclerotial extracts	1105:1127	The sclerotial extracts contain phenolics, terpenoids, and glucan.
26598045	9	17	dep	mmol	1317:1320	arg1	0.015					1311:1315	0.015	1311:1315	0.015	1311:1315	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	4	18	theme	reducing	680:687	arg1	power					701:705	ferric reducing antioxidant power	673:705	ferric reducing antioxidant power	673:705	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	9	19	theme	ferric	1176:1181	arg1	values					1210:1215	The ferric reducing antioxidant power values	1172:1215	The ferric reducing antioxidant power values of the various sclerotial extracts	1172:1250	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	4	20	theme	sclerotial	633:642	arg1	extracts					644:651	the sclerotial extracts	629:651	the sclerotial extracts	629:651	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	9	21	theme	antioxidant	1192:1202	arg1	values					1210:1215	The ferric reducing antioxidant power values	1172:1215	The ferric reducing antioxidant power values of the various sclerotial extracts	1172:1250	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	3	22	dep	contain	403:409	arg1	candidate					490:498	a potential candidate	478:498	a potential candidate as a dietary supplement	478:522	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	3	22	dep	contain	403:409	arg1	nontoxic					465:472	nontoxic	465:472	nontoxic	465:472	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	6	23	theme	small	956:960	arg1	amounts					962:968	small amounts	956:968	small amounts of fat, calories, and sugar	956:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	23	theme	small	956:960	arg1	calories					978:985	calories	978:985	calories	978:985	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	23	theme	small	956:960	arg1	sugar					992:996	sugar	992:996	sugar	992:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	23	theme	small	956:960	arg1	fat					973:975	fat	973:975	fat	973:975	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	1	24	theme	Milk	154:157	arg1	Mushroom					159:166	The Tiger Milk Mushroom	144:166	The Tiger Milk Mushroom (Lignosus spp.)	144:182	The Tiger Milk Mushroom (Lignosus spp.)
26598045	1	24	theme	Milk	154:157	arg1	spp					178:180	spp	178:180	spp	178:180	The Tiger Milk Mushroom (Lignosus spp.)
26598045	10	25	from	dose	1626:1629	arg1	toxic					1613:1617	toxic	1613:1617	toxic	1613:1617	Acute toxicity assessment indicated that L. tigris E sclerotial powder was not toxic at the dose of 2000 mg kg(-1).
26598045	10	26	dep	L.	1575:1576	arg1	tigris					1578:1583	tigris	1578:1583	tigris	1578:1583	Acute toxicity assessment indicated that L. tigris E sclerotial powder was not toxic at the dose of 2000 mg kg(-1).
26598045	9	27	theme	scavenging	1414:1423	arg1	activities					1425:1434	superoxide anion radical scavenging activities	1389:1434	superoxide anion radical scavenging activities	1389:1434	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	11	28	theme	functional	1729:1738	arg1	food					1740:1743	a functional food	1727:1743	a functional food	1727:1743	In conclusion, L. tigris E sclerotium has the potential to be developed into a functional food and nutraceutical.
26598045	9	29	dep	extract	1336:1342	arg1	mmol					1317:1320	mmol	1317:1320	mmol	1317:1320	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	3	30	theme	E	385:385	arg1	sclerotium					388:397	Lignosus tigris (cultivar E) sclerotium	359:397	Lignosus tigris (cultivar E) sclerotium	359:397	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	0	31	theme	Milk	102:105	arg1	E					141:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	9	32	theme	sclerotial	1232:1241	arg1	extracts					1243:1250	the various sclerotial extracts	1220:1250	the various sclerotial extracts	1220:1250	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	9	33	theme	superoxide	1389:1398	arg1	activities					1425:1434	superoxide anion radical scavenging activities	1389:1434	superoxide anion radical scavenging activities	1389:1434	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	0	34	theme	Nutritional	0:10	arg1	composition					12:22	Nutritional composition	0:22	Nutritional composition	0:22	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	6	35	theme	sugar	992:996	arg1	amounts					962:968	small amounts	956:968	small amounts of fat, calories, and sugar	956:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	35	theme	sugar	992:996	arg1	calories					978:985	calories	978:985	calories	978:985	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	35	theme	sugar	992:996	arg1	sugar					992:996	sugar	992:996	sugar	992:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	35	theme	sugar	992:996	arg1	fat					973:975	fat	973:975	fat	973:975	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	7	36	theme	protein	1084:1090	arg1	score					1092:1096	a protein score	1082:1096	a protein score of 47	1082:1102	The amino acid composition of the protein contains all essential amino acids, with a protein score of 47.
26598045	3	37	theme	Lignosus	359:366	arg1	sclerotium					388:397	Lignosus tigris (cultivar E) sclerotium	359:397	Lignosus tigris (cultivar E) sclerotium	359:397	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	9	38	theme	cold	1264:1267	arg1	water					1269:1273	cold water	1264:1273	cold water	1264:1273	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	4	39	theme	scavenging	768:777	arg1	assays					779:784	radical scavenging assays	760:784	radical scavenging assays	760:784	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	3	40	theme	nutritional	416:426	arg1	value					428:432	high nutritional value	411:432	high nutritional value	411:432	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	9	41	theme	mmol	1481:1484	arg1	equivalents					1493:1503	0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents	1448:1503	0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract	1448:1517	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	4	42	theme	amino	542:546	arg1	compositions					553:564	The chemical and amino acid compositions	525:564	compositions	553:564	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	11	43	theme	tigris	1668:1673	arg1	sclerotium					1677:1686	L. tigris E sclerotium	1665:1686	L. tigris E sclerotium	1665:1686	In conclusion, L. tigris E sclerotium has the potential to be developed into a functional food and nutraceutical.
26598045	10	44	theme	E	1585:1585	arg1	powder					1598:1603	L. tigris E sclerotial powder	1575:1603	L. tigris E sclerotial powder	1575:1603	Acute toxicity assessment indicated that L. tigris E sclerotial powder was not toxic at the dose of 2000 mg kg(-1).
26598045	7	45	theme	amino	1064:1068	arg1	acids					1070:1074	all essential amino acids	1050:1074	all essential amino acids	1050:1074	The amino acid composition of the protein contains all essential amino acids, with a protein score of 47.
26598045	9	46	theme	equivalents	1493:1503	arg1	-1					1507:1508	-1	1507:1508	-1	1507:1508	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	9	46	theme	equivalents	1493:1503	arg1	g					1505:1505	0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g	1448:1505	0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract	1448:1517	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	2	47	theme	illnesses	312:320	arg1	illnesses					312:320	illnesses	312:320	illnesses	312:320	is an important medicinal mushroom in Southeast Asia and has been consumed frequently by the natives as a cure for a variety of illnesses.
26598045	2	47	theme	illnesses	312:320	arg1	variety					301:307	a variety	299:307	a variety of illnesses	299:320	is an important medicinal mushroom in Southeast Asia and has been consumed frequently by the natives as a cure for a variety of illnesses.
26598045	7	48	theme	protein	1033:1039	arg1	composition					1014:1024	The amino acid composition	999:1024	The amino acid composition of the protein	999:1039	The amino acid composition of the protein contains all essential amino acids, with a protein score of 47.
26598045	3	49	theme	potential	480:488	arg1	candidate					490:498	a potential candidate	478:498	a potential candidate as a dietary supplement	478:522	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	6	50	with	carbohydrate	909:920	arg1	amounts					962:968	small amounts	956:968	small amounts of fat, calories, and sugar	956:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	50	with	carbohydrate	909:920	arg1	calories					978:985	calories	978:985	calories	978:985	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	50	with	carbohydrate	909:920	arg1	sugar					992:996	sugar	992:996	sugar	992:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	50	with	carbohydrate	909:920	arg1	fat					973:975	fat	973:975	fat	973:975	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	9	51	theme	min	1322:1324	arg1	extract					1336:1342	0.008 to 0.015 mmol min(-1) g(-1) extract	1302:1342	0.008 to 0.015 mmol min(-1) g(-1) extract	1302:1342	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	6	52	with	protein	923:929	arg1	amounts					962:968	small amounts	956:968	small amounts of fat, calories, and sugar	956:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	52	with	protein	923:929	arg1	calories					978:985	calories	978:985	calories	978:985	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	52	with	protein	923:929	arg1	sugar					992:996	sugar	992:996	sugar	992:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	52	with	protein	923:929	arg1	fat					973:975	fat	973:975	fat	973:975	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	7	53	theme	acid	1009:1012	arg1	composition					1014:1024	The amino acid composition	999:1024	The amino acid composition of the protein	999:1039	The amino acid composition of the protein contains all essential amino acids, with a protein score of 47.
26598045	3	54	theme	dietary	505:511	arg1	supplement					513:522	a dietary supplement	503:522	a dietary supplement	503:522	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	0	55	theme	tigris	125:130	arg1	E					141:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	9	56	theme	g	1330:1330	arg1	extract					1336:1342	0.008 to 0.015 mmol min(-1) g(-1) extract	1302:1342	0.008 to 0.015 mmol min(-1) g(-1) extract	1302:1342	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	9	57	dep	ranged	1290:1295	arg1	water					1257:1261	hot water	1253:1261	hot water	1253:1261	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	9	57	dep	ranged	1290:1295	arg1	water					1269:1273	cold water	1264:1273	cold water	1264:1273	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	9	57	dep	ranged	1290:1295	arg1	methanol					1280:1287	methanol	1280:1287	methanol	1280:1287	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	9	58	theme	anion	1400:1404	arg1	activities					1425:1434	superoxide anion radical scavenging activities	1389:1434	superoxide anion radical scavenging activities	1389:1434	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	11	59	theme	L.	1665:1666	arg1	sclerotium					1677:1686	L. tigris E sclerotium	1665:1686	L. tigris E sclerotium	1665:1686	In conclusion, L. tigris E sclerotium has the potential to be developed into a functional food and nutraceutical.
26598045	0	60	theme	E	141:141	arg1	sclerotium					82:91	the sclerotium	78:91	the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E	78:141	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	10	61	theme	kg	1642:1643	arg1	dose					1626:1629	the dose	1622:1629	the dose of 2000 mg kg(-1)	1622:1647	Acute toxicity assessment indicated that L. tigris E sclerotial powder was not toxic at the dose of 2000 mg kg(-1).
26598045	5	62	theme	E	819:819	arg1	sclerotium					821:830	the L. tigris E sclerotium	805:830	the L. tigris E sclerotium	805:830	Acute toxicity of the L. tigris E sclerotium was assessed using a rat model study.
26598045	2	63	theme	medicinal	200:208	arg1	mushroom					210:217	an important medicinal mushroom	187:217	an important medicinal mushroom	187:217	is an important medicinal mushroom in Southeast Asia and has been consumed frequently by the natives as a cure for a variety of illnesses.
26598045	9	64	dep	0.015	1311:1315	arg1	to					1308:1309	to	1308:1309	to	1308:1309	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	4	65	dep	power	701:705	arg1	assays					779:784	radical scavenging assays	760:784	radical scavenging assays	760:784	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	6	66	theme	fat	973:975	arg1	amounts					962:968	small amounts	956:968	small amounts of fat, calories, and sugar	956:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	66	theme	fat	973:975	arg1	calories					978:985	calories	978:985	calories	978:985	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	66	theme	fat	973:975	arg1	sugar					992:996	sugar	992:996	sugar	992:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	66	theme	fat	973:975	arg1	fat					973:975	fat	973:975	fat	973:975	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	4	67	theme	ferric	673:678	arg1	power					701:705	ferric reducing antioxidant power	673:705	ferric reducing antioxidant power	673:705	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	10	68	theme	toxicity	1540:1547	arg1	assessment					1549:1558	Acute toxicity assessment	1534:1558	Acute toxicity assessment	1534:1558	Acute toxicity assessment indicated that L. tigris E sclerotial powder was not toxic at the dose of 2000 mg kg(-1).
26598045	9	69	theme	reducing	1183:1190	arg1	values					1210:1215	The ferric reducing antioxidant power values	1172:1215	The ferric reducing antioxidant power values of the various sclerotial extracts	1172:1250	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	5	70	theme	L.	809:810	arg1	sclerotium					821:830	the L. tigris E sclerotium	805:830	the L. tigris E sclerotium	805:830	Acute toxicity of the L. tigris E sclerotium was assessed using a rat model study.
26598045	5	71	theme	rat	853:855	arg1	model					857:861	a rat model	851:861	a rat model study	851:867	Acute toxicity of the L. tigris E sclerotium was assessed using a rat model study.
26598045	6	72	with	fibers	944:949	arg1	amounts					962:968	small amounts	956:968	small amounts of fat, calories, and sugar	956:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	72	with	fibers	944:949	arg1	calories					978:985	calories	978:985	calories	978:985	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	72	with	fibers	944:949	arg1	sugar					992:996	sugar	992:996	sugar	992:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	72	with	fibers	944:949	arg1	fat					973:975	fat	973:975	fat	973:975	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	9	73	dep	0.13	1456:1459	arg1	to					1453:1454	to	1453:1454	to	1453:1454	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	7	74	contain	contains	1041:1048	arg1	composition					1014:1024	The amino acid composition	999:1024	The amino acid composition of the protein	999:1039	The amino acid composition of the protein contains all essential amino acids, with a protein score of 47.
26598045	7	74	contain	contains	1041:1048	arg2	acids					1070:1074	all essential amino acids	1050:1074	all essential amino acids	1050:1074	The amino acid composition of the protein contains all essential amino acids, with a protein score of 47.
26598045	0	75	theme	sclerotium	82:91	arg1	evaluation					64:73	toxicology evaluation	53:73	toxicology evaluation	53:73	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	0	75	theme	sclerotium	82:91	arg1	composition					12:22	Nutritional composition	0:22	Nutritional composition	0:22	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	0	75	theme	sclerotium	82:91	arg1	properties					37:46	antioxidant properties	25:46	antioxidant properties	25:46	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	9	76	theme	power	1204:1208	arg1	values					1210:1215	The ferric reducing antioxidant power values	1172:1215	The ferric reducing antioxidant power values of the various sclerotial extracts	1172:1250	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	1	77	theme	Tiger	148:152	arg1	Mushroom					159:166	The Tiger Milk Mushroom	144:166	The Tiger Milk Mushroom (Lignosus spp.)	144:182	The Tiger Milk Mushroom (Lignosus spp.)
26598045	1	77	theme	Tiger	148:152	arg1	spp					178:180	spp	178:180	spp	178:180	The Tiger Milk Mushroom (Lignosus spp.)
26598045	0	78	theme	Lignosus	116:123	arg1	E					141:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	4	79	theme	extracts	644:651	arg1	activities					615:624	antioxidant activities	603:624	antioxidant activities of the sclerotial extracts	603:651	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	0	80	theme	Tiger	96:100	arg1	E					141:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	9	81	theme	radical	1406:1412	arg1	activities					1425:1434	superoxide anion radical scavenging activities	1389:1434	superoxide anion radical scavenging activities	1389:1434	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	6	82	theme	dietary	936:942	arg1	fibers					944:949	dietary fibers	936:949	dietary fibers	936:949	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	0	83	theme	Mushroom	107:114	arg1	E					141:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Tiger Milk Mushroom Lignosus tigris cultivar E	96:141	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	9	84	theme	various	1224:1230	arg1	extracts					1243:1250	the various sclerotial extracts	1220:1250	the various sclerotial extracts	1220:1250	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	4	85	theme	superoxide	743:752	arg1	anion					754:758	superoxide anion	743:758	superoxide anion	743:758	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	7	86	theme	47	1101:1102	arg1	score					1092:1096	a protein score	1082:1096	a protein score of 47	1082:1102	The amino acid composition of the protein contains all essential amino acids, with a protein score of 47.
26598045	5	87	theme	Acute	787:791	arg1	toxicity					793:800	Acute toxicity	787:800	Acute toxicity of the L. tigris E sclerotium	787:830	Acute toxicity of the L. tigris E sclerotium was assessed using a rat model study.
26598045	9	88	theme	extracts	1243:1250	arg1	values					1210:1215	The ferric reducing antioxidant power values	1172:1215	The ferric reducing antioxidant power values of the various sclerotial extracts	1172:1250	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	2	89	from	mushroom	210:217	arg1	Asia					232:235	Asia	232:235	Asia	232:235	is an important medicinal mushroom in Southeast Asia and has been consumed frequently by the natives as a cure for a variety of illnesses.
26598045	6	90	from	fibers	944:949	arg1	rich					901:904	rich	901:904	rich	901:904	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	90	from	fibers	944:949	arg1	sclerotium					874:883	The sclerotium	870:883	The sclerotium	870:883	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	4	91	theme	antioxidant	689:699	arg1	power					701:705	ferric reducing antioxidant power	673:705	ferric reducing antioxidant power	673:705	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	3	92	theme	cultivar	376:383	arg1	sclerotium					388:397	Lignosus tigris (cultivar E) sclerotium	359:397	Lignosus tigris (cultivar E) sclerotium	359:397	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	0	93	theme	antioxidant	25:35	arg1	properties					37:46	antioxidant properties	25:46	antioxidant properties	25:46	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	9	94	theme	hot	1253:1255	arg1	water					1257:1261	hot water	1253:1261	hot water	1253:1261	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
26598045	6	95	from	carbohydrate	909:920	arg1	rich					901:904	rich	901:904	rich	901:904	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	95	from	carbohydrate	909:920	arg1	sclerotium					874:883	The sclerotium	870:883	The sclerotium	870:883	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	5	96	dep	model	857:861	arg1	study					863:867	study	863:867	study	863:867	Acute toxicity of the L. tigris E sclerotium was assessed using a rat model study.
26598045	3	97	theme	high	411:414	arg1	value					428:432	high nutritional value	411:432	high nutritional value	411:432	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	11	98	contain	has	1688:1690	arg1	sclerotium					1677:1686	L. tigris E sclerotium	1665:1686	L. tigris E sclerotium	1665:1686	In conclusion, L. tigris E sclerotium has the potential to be developed into a functional food and nutraceutical.
26598045	11	98	contain	has	1688:1690	arg2	potential					1696:1704	the potential to be developed into a functional food and nutraceutical	1692:1761	the potential to be developed into a functional food and nutraceutical	1692:1761	In conclusion, L. tigris E sclerotium has the potential to be developed into a functional food and nutraceutical.
26598045	6	99	theme	calories	978:985	arg1	amounts					962:968	small amounts	956:968	small amounts of fat, calories, and sugar	956:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	99	theme	calories	978:985	arg1	calories					978:985	calories	978:985	calories	978:985	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	99	theme	calories	978:985	arg1	sugar					992:996	sugar	992:996	sugar	992:996	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	99	theme	calories	978:985	arg1	fat					973:975	fat	973:975	fat	973:975	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	3	100	theme	tigris	368:373	arg1	sclerotium					388:397	Lignosus tigris (cultivar E) sclerotium	359:397	Lignosus tigris (cultivar E) sclerotium	359:397	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	6	101	from	protein	923:929	arg1	rich					901:904	rich	901:904	rich	901:904	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	101	from	protein	923:929	arg1	sclerotium					874:883	The sclerotium	870:883	The sclerotium	870:883	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	0	102	theme	toxicology	53:62	arg1	evaluation					64:73	toxicology evaluation	53:73	toxicology evaluation	53:73	Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E.
26598045	3	103	theme	antioxidant	438:448	arg1	properties					450:459	antioxidant properties	438:459	antioxidant properties	438:459	In this study, we hypothesized that Lignosus tigris (cultivar E) sclerotium may contain high nutritional value and antioxidant properties, is nontoxic and a potential candidate as a dietary supplement.
26598045	4	104	theme	acid	548:551	arg1	compositions					553:564	The chemical and amino acid compositions	525:564	compositions	553:564	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	6	105	from	rich	901:904	arg1	fibers					944:949	dietary fibers	936:949	dietary fibers	936:949	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	105	from	rich	901:904	arg1	protein					923:929	protein	923:929	protein	923:929	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	6	105	from	rich	901:904	arg1	carbohydrate					909:920	carbohydrate	909:920	carbohydrate	909:920	The sclerotium was found to be rich in carbohydrate, protein, and dietary fibers with small amounts of fat, calories, and sugar.
26598045	4	106	theme	radical	760:766	arg1	assays					779:784	radical scavenging assays	760:784	radical scavenging assays	760:784	The chemical and amino acid compositions of the sclerotium were evaluated and antioxidant activities of the sclerotial extracts were assessed using ferric reducing antioxidant power; 1,1-diphenyl-2-picrylhydrazyl; and superoxide anion radical scavenging assays.
26598045	10	107	theme	Acute	1534:1538	arg1	assessment					1549:1558	Acute toxicity assessment	1534:1558	Acute toxicity assessment	1534:1558	Acute toxicity assessment indicated that L. tigris E sclerotial powder was not toxic at the dose of 2000 mg kg(-1).
26598045	9	108	theme	Trolox	1486:1491	arg1	equivalents					1493:1503	0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents	1448:1503	0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract	1448:1517	The ferric reducing antioxidant power values of the various sclerotial extracts (hot water, cold water, and methanol) ranged from 0.008 to 0.015 mmol min(-1) g(-1) extract, while the 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radical scavenging activities ranged from 0.11 to 0.13, and -2.81 to 9.613 mmol Trolox equivalents g(-1) extract, respectively.
28956650	8	0	from	time	1248:1251	arg1	blood					1266:1270	the blood	1262:1270	the blood	1262:1270	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	8	1	theme	P4	1230:1231	arg1	time					1248:1251	∼2-fold higher P4 mean residence time	1215:1251	∼2-fold higher P4 mean residence time (MRT) in the blood	1215:1270	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	8	1	theme	P4	1230:1231	arg1	MRT					1254:1256	MRT	1254:1256	MRT	1254:1256	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	0	2	theme	Mucoadhesive	0:11	arg1	tablets					13:19	Mucoadhesive tablets	0:19	Mucoadhesive tablets for the vaginal delivery of progesterone: in vitro evaluation and pharmacokinetics/pharmacodynamics in female rabbits.	0:138	Mucoadhesive tablets for the vaginal delivery of progesterone: in vitro evaluation and pharmacokinetics/pharmacodynamics in female rabbits.
28956650	1	3	theme	vaginal	190:196	arg1	delivery					198:205	the vaginal delivery	186:205	the vaginal delivery of progesterone (P4)	186:226	OBJECTIVE To develop mucoadhesive tablets for the vaginal delivery of progesterone (P4) to overcome its low oral bioavailability resulting from drug hydrophobicity and extensive hepatic metabolism.
28956650	8	4	theme	residence	1238:1246	arg1	time					1248:1251	∼2-fold higher P4 mean residence time	1215:1251	∼2-fold higher P4 mean residence time (MRT) in the blood	1215:1270	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	8	4	theme	residence	1238:1246	arg1	MRT					1254:1256	MRT	1254:1256	MRT	1254:1256	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	8	5	theme	higher	1283:1288	arg1	bioavailability					1290:1304	5-fold higher bioavailability	1276:1304	5-fold higher bioavailability	1276:1304	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	5	6	dep	in	826:827	arg1	vitro					829:833	vitro	829:833	vitro	829:833	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	8	7	dep	In	1146:1147	arg1	vivo					1149:1152	vivo	1149:1152	vivo	1149:1152	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	7	8	theme	drug	1122:1125	arg1	release					1127:1133	the drug release	1118:1133	the drug release	1118:1133	Most tablets exhibited burst release (∼25%) during the first 2 h but sustained the drug release for ∼48 h.
28956650	6	9	theme	alginate	1021:1028	arg1	mixture					1030:1036	20% chitosan-10% alginate mixture	1004:1036	20% chitosan-10% alginate mixture	1004:1036	Highest swelling index and mucoadhesion time were detected for tablets containing 20% chitosan-10% alginate mixture.
28956650	3	10	theme	tablets	467:473	arg1	mucoadhesion					512:523	mucoadhesion	512:523	mucoadhesion	512:523	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	3	10	theme	tablets	467:473	arg1	properties					484:493	The tablets physical properties	463:493	The tablets physical properties	463:493	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	3	10	theme	tablets	467:473	arg1	index					505:509	swelling index	496:509	swelling index	496:509	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	3	10	theme	tablets	467:473	arg1	kinetics					542:549	drug release kinetics	529:549	drug release kinetics	529:549	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	8	11	theme	oral	1320:1323	arg1	P4					1325:1326	oral P4	1320:1326	oral P4	1320:1326	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	5	12	theme	satisfactory	773:784	arg1	properties					795:804	satisfactory physical properties	773:804	satisfactory physical properties	773:804	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	6	13	theme	chitosan-10	1008:1018	arg1	%					1006:1006	%	1006:1006	%	1006:1006	Highest swelling index and mucoadhesion time were detected for tablets containing 20% chitosan-10% alginate mixture.
28956650	6	14	theme	20	1004:1005	arg1	%					1006:1006	%	1006:1006	%	1006:1006	Highest swelling index and mucoadhesion time were detected for tablets containing 20% chitosan-10% alginate mixture.
28956650	2	15	dep	METHODS	338:344	arg1	prepared					363:370	prepared	363:370	were prepared using mixtures of P4/Pluronic® F-127 solid dispersion and different mucoadhesive polymers	358:460	METHODS The tablets were prepared using mixtures of P4/Pluronic® F-127 solid dispersion and different mucoadhesive polymers.
28956650	7	16	theme	burst	1062:1066	arg1	release					1068:1074	burst release	1062:1074	burst release (∼25%)	1062:1081	Most tablets exhibited burst release (∼25%) during the first 2 h but sustained the drug release for ∼48 h.
28956650	7	16	theme	burst	1062:1066	arg1	%					1080:1080	∼25%	1077:1080	∼25%	1077:1080	Most tablets exhibited burst release (∼25%) during the first 2 h but sustained the drug release for ∼48 h.
28956650	1	17	theme	mucoadhesive	161:172	arg1	tablets					174:180	mucoadhesive tablets	161:180	mucoadhesive tablets for the vaginal delivery of progesterone (P4)	161:226	OBJECTIVE To develop mucoadhesive tablets for the vaginal delivery of progesterone (P4) to overcome its low oral bioavailability resulting from drug hydrophobicity and extensive hepatic metabolism.
28956650	9	18	theme	same	1338:1341	arg1	tablets					1343:1349	same tablets	1338:1349	same tablets	1338:1349	Further, same tablets showed 2-fold higher myometrium thickness in rabbit uterus compared with IM P4 injection.
28956650	3	19	theme	physical	475:482	arg1	mucoadhesion					512:523	mucoadhesion	512:523	mucoadhesion	512:523	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	3	19	theme	physical	475:482	arg1	properties					484:493	The tablets physical properties	463:493	The tablets physical properties	463:493	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	3	19	theme	physical	475:482	arg1	index					505:509	swelling index	496:509	swelling index	496:509	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	3	19	theme	physical	475:482	arg1	kinetics					542:549	drug release kinetics	529:549	drug release kinetics	529:549	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	10	20	theme	vaginal	1510:1516	arg1	tablets					1518:1524	these mucoadhesive vaginal tablets	1491:1524	these mucoadhesive vaginal tablets	1491:1524	CONCLUSION These results confirm the potential of these mucoadhesive vaginal tablets to enhance P4 efficacy and avoid the side effects associated with IM injection.
28956650	2	21	theme	solid	409:413	arg1	dispersion					415:424	P4/Pluronic® F-127 solid dispersion	390:424	P4/Pluronic® F-127 solid dispersion	390:424	METHODS The tablets were prepared using mixtures of P4/Pluronic® F-127 solid dispersion and different mucoadhesive polymers.
28956650	5	22	theme	mucoadhesion	866:877	arg1	time					879:882	ex vivo mucoadhesion time	858:882	ex vivo mucoadhesion time	858:882	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	9	23	theme	higher	1365:1370	arg1	thickness					1383:1391	2-fold higher myometrium thickness	1358:1391	2-fold higher myometrium thickness	1358:1391	Further, same tablets showed 2-fold higher myometrium thickness in rabbit uterus compared with IM P4 injection.
28956650	5	24	contain	had	769:771	arg1	tablets					761:767	The tablets	757:767	The tablets	757:767	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	5	24	contain	had	769:771	arg2	properties					795:804	satisfactory physical properties	773:804	satisfactory physical properties	773:804	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	10	25	theme	P4	1537:1538	arg1	efficacy					1540:1547	P4 efficacy	1537:1547	P4 efficacy	1537:1547	CONCLUSION These results confirm the potential of these mucoadhesive vaginal tablets to enhance P4 efficacy and avoid the side effects associated with IM injection.
28956650	2	26	theme	mucoadhesive	440:451	arg1	polymers					453:460	different mucoadhesive polymers	430:460	different mucoadhesive polymers	430:460	METHODS The tablets were prepared using mixtures of P4/Pluronic® F-127 solid dispersion and different mucoadhesive polymers.
28956650	0	27	theme	vaginal	29:35	arg1	delivery					37:44	the vaginal delivery	25:44	the vaginal delivery of progesterone	25:60	Mucoadhesive tablets for the vaginal delivery of progesterone: in vitro evaluation and pharmacokinetics/pharmacodynamics in female rabbits.
28956650	9	28	theme	rabbit	1396:1401	arg1	uterus					1403:1408	rabbit uterus	1396:1408	rabbit uterus	1396:1408	Further, same tablets showed 2-fold higher myometrium thickness in rabbit uterus compared with IM P4 injection.
28956650	6	29	theme	Highest	922:928	arg1	index					939:943	Highest swelling index	922:943	Highest swelling index	922:943	Highest swelling index and mucoadhesion time were detected for tablets containing 20% chitosan-10% alginate mixture.
28956650	1	30	theme	hepatic	318:324	arg1	metabolism					326:335	extensive hepatic metabolism	308:335	extensive hepatic metabolism	308:335	OBJECTIVE To develop mucoadhesive tablets for the vaginal delivery of progesterone (P4) to overcome its low oral bioavailability resulting from drug hydrophobicity and extensive hepatic metabolism.
28956650	3	31	theme	drug	529:532	arg1	properties					484:493	The tablets physical properties	463:493	The tablets physical properties	463:493	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	3	31	theme	drug	529:532	arg1	kinetics					542:549	drug release kinetics	529:549	drug release kinetics	529:549	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	5	32	theme	ex	858:859	arg1	time					879:882	ex vivo mucoadhesion time	858:882	ex vivo mucoadhesion time	858:882	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	8	33	theme	In	1146:1147	arg1	study					1154:1158	In vivo study	1146:1158	In vivo study	1146:1158	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	10	34	dep	CONCLUSION	1441:1450	arg1	confirm					1466:1472	confirm	1466:1472	confirm the potential of these mucoadhesive vaginal tablets to enhance P4 efficacy and avoid the side effects associated with IM injection	1466:1603	CONCLUSION These results confirm the potential of these mucoadhesive vaginal tablets to enhance P4 efficacy and avoid the side effects associated with IM injection.
28956650	9	35	theme	IM	1424:1425	arg1	injection					1430:1438	IM P4 injection	1424:1438	IM P4 injection	1424:1438	Further, same tablets showed 2-fold higher myometrium thickness in rabbit uterus compared with IM P4 injection.
28956650	3	36	theme	swelling	496:503	arg1	properties					484:493	The tablets physical properties	463:493	The tablets physical properties	463:493	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	3	36	theme	swelling	496:503	arg1	index					505:509	swelling index	496:509	swelling index	496:509	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	2	37	theme	P4/Pluronic®	390:401	arg1	dispersion					415:424	P4/Pluronic® F-127 solid dispersion	390:424	P4/Pluronic® F-127 solid dispersion	390:424	METHODS The tablets were prepared using mixtures of P4/Pluronic® F-127 solid dispersion and different mucoadhesive polymers.
28956650	4	38	theme	P4	567:568	arg1	properties					606:615	P4 pharmacokinetic and pharmacodynamic properties	567:615	P4 pharmacokinetic and pharmacodynamic properties	567:615	P4 pharmacokinetic and pharmacodynamic properties were evaluated in female rabbits and compared with vaginal micronized P4 tablets and intramuscular (IM) P4 injection, respectively.
28956650	1	39	theme	oral	248:251	arg1	bioavailability					253:267	its low oral bioavailability	240:267	its low oral bioavailability resulting from drug hydrophobicity and extensive hepatic metabolism	240:335	OBJECTIVE To develop mucoadhesive tablets for the vaginal delivery of progesterone (P4) to overcome its low oral bioavailability resulting from drug hydrophobicity and extensive hepatic metabolism.
28956650	8	40	theme	mucoadhesive	1190:1201	arg1	tablets					1203:1209	chitosan-alginate mucoadhesive tablets	1172:1209	chitosan-alginate mucoadhesive tablets	1172:1209	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	4	41	theme	female	635:640	arg1	rabbits					642:648	female rabbits	635:648	female rabbits	635:648	P4 pharmacokinetic and pharmacodynamic properties were evaluated in female rabbits and compared with vaginal micronized P4 tablets and intramuscular (IM) P4 injection, respectively.
28956650	8	42	contain	had	1211:1213	arg2	MRT					1254:1256	MRT	1254:1256	MRT	1254:1256	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	8	42	contain	had	1211:1213	arg1	tablets					1203:1209	chitosan-alginate mucoadhesive tablets	1172:1209	chitosan-alginate mucoadhesive tablets	1172:1209	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	8	42	contain	had	1211:1213	arg2	bioavailability					1290:1304	5-fold higher bioavailability	1276:1304	5-fold higher bioavailability	1276:1304	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	8	42	contain	had	1211:1213	arg2	time					1248:1251	∼2-fold higher P4 mean residence time	1215:1251	∼2-fold higher P4 mean residence time (MRT) in the blood	1215:1270	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	8	43	theme	higher	1223:1228	arg1	time					1248:1251	∼2-fold higher P4 mean residence time	1215:1251	∼2-fold higher P4 mean residence time (MRT) in the blood	1215:1270	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	8	43	theme	higher	1223:1228	arg1	MRT					1254:1256	MRT	1254:1256	MRT	1254:1256	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	6	44	theme	mucoadhesion	949:960	arg1	time					962:965	mucoadhesion time	949:965	mucoadhesion time	949:965	Highest swelling index and mucoadhesion time were detected for tablets containing 20% chitosan-10% alginate mixture.
28956650	5	45	dep	ex	858:859	arg1	vivo					861:864	vivo	861:864	vivo	861:864	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	4	46	theme	IM	717:718	arg1	injection					724:732	intramuscular (IM) P4 injection	702:732	intramuscular (IM) P4 injection	702:732	P4 pharmacokinetic and pharmacodynamic properties were evaluated in female rabbits and compared with vaginal micronized P4 tablets and intramuscular (IM) P4 injection, respectively.
28956650	8	47	theme	mean	1233:1236	arg1	time					1248:1251	∼2-fold higher P4 mean residence time	1215:1251	∼2-fold higher P4 mean residence time (MRT) in the blood	1215:1270	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	8	47	theme	mean	1233:1236	arg1	MRT					1254:1256	MRT	1254:1256	MRT	1254:1256	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	6	48	contain	containing	993:1002	arg2	mixture					1030:1036	20% chitosan-10% alginate mixture	1004:1036	20% chitosan-10% alginate mixture	1004:1036	Highest swelling index and mucoadhesion time were detected for tablets containing 20% chitosan-10% alginate mixture.
28956650	6	48	contain	containing	993:1002	arg1	tablets					985:991	tablets	985:991	tablets containing 20% chitosan-10% alginate mixture	985:1036	Highest swelling index and mucoadhesion time were detected for tablets containing 20% chitosan-10% alginate mixture.
28956650	1	49	theme	progesterone	210:221	arg1	delivery					198:205	the vaginal delivery	186:205	the vaginal delivery of progesterone (P4)	186:226	OBJECTIVE To develop mucoadhesive tablets for the vaginal delivery of progesterone (P4) to overcome its low oral bioavailability resulting from drug hydrophobicity and extensive hepatic metabolism.
28956650	0	50	from	evaluation	72:81	arg1	rabbits					131:137	female rabbits	124:137	female rabbits	124:137	Mucoadhesive tablets for the vaginal delivery of progesterone: in vitro evaluation and pharmacokinetics/pharmacodynamics in female rabbits.
28956650	4	51	theme	P4	721:722	arg1	injection					724:732	intramuscular (IM) P4 injection	702:732	intramuscular (IM) P4 injection	702:732	P4 pharmacokinetic and pharmacodynamic properties were evaluated in female rabbits and compared with vaginal micronized P4 tablets and intramuscular (IM) P4 injection, respectively.
28956650	5	52	theme	tablet	902:907	arg1	composition					909:919	tablet composition	902:919	tablet composition	902:919	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	6	53	dep	%	1019:1019	arg1	%					1006:1006	%	1006:1006	%	1006:1006	Highest swelling index and mucoadhesion time were detected for tablets containing 20% chitosan-10% alginate mixture.
28956650	4	54	theme	intramuscular	702:714	arg1	injection					724:732	intramuscular (IM) P4 injection	702:732	intramuscular (IM) P4 injection	702:732	P4 pharmacokinetic and pharmacodynamic properties were evaluated in female rabbits and compared with vaginal micronized P4 tablets and intramuscular (IM) P4 injection, respectively.
28956650	7	55	theme	first	1094:1098	arg1	2 h					1100:1102	the first 2 h	1090:1102	the first 2 h	1090:1102	Most tablets exhibited burst release (∼25%) during the first 2 h but sustained the drug release for ∼48 h.
28956650	6	56	dep	mixture	1030:1036	arg1	%					1019:1019	%	1019:1019	%	1019:1019	Highest swelling index and mucoadhesion time were detected for tablets containing 20% chitosan-10% alginate mixture.
28956650	0	57	from	pharmacokinetics/pharmacodynamics	87:119	arg1	rabbits					131:137	female rabbits	124:137	female rabbits	124:137	Mucoadhesive tablets for the vaginal delivery of progesterone: in vitro evaluation and pharmacokinetics/pharmacodynamics in female rabbits.
28956650	0	58	theme	in	63:64	arg1	evaluation					72:81	in vitro evaluation	63:81	in vitro evaluation	63:81	Mucoadhesive tablets for the vaginal delivery of progesterone: in vitro evaluation and pharmacokinetics/pharmacodynamics in female rabbits.
28956650	5	59	theme	in	826:827	arg1	force					848:852	in vitro mucoadhesion force	826:852	in vitro mucoadhesion force	826:852	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	8	60	from	bioavailability	1290:1304	arg1	blood					1266:1270	the blood	1262:1270	the blood	1262:1270	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	1	61	dep	drug	284:287	arg1	hydrophobicity					289:302	hydrophobicity	289:302	hydrophobicity	289:302	OBJECTIVE To develop mucoadhesive tablets for the vaginal delivery of progesterone (P4) to overcome its low oral bioavailability resulting from drug hydrophobicity and extensive hepatic metabolism.
28956650	5	62	theme	physical	786:793	arg1	properties					795:804	satisfactory physical properties	773:804	satisfactory physical properties	773:804	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	7	63	theme	Most	1039:1042	arg1	tablets					1044:1050	Most tablets	1039:1050	Most tablets	1039:1050	Most tablets exhibited burst release (∼25%) during the first 2 h but sustained the drug release for ∼48 h.
28956650	5	64	dep	RESULTS	749:755	arg1	dependent					889:897	dependent	889:897	dependent	889:897	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	5	64	dep	RESULTS	749:755	arg1	had					769:771	had	769:771	had satisfactory physical properties	769:804	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	0	65	theme	female	124:129	arg1	rabbits					131:137	female rabbits	124:137	female rabbits	124:137	Mucoadhesive tablets for the vaginal delivery of progesterone: in vitro evaluation and pharmacokinetics/pharmacodynamics in female rabbits.
28956650	10	66	theme	tablets	1518:1524	arg1	potential					1478:1486	the potential	1474:1486	the potential of these mucoadhesive vaginal tablets to enhance P4 efficacy and avoid the side effects associated with IM injection	1474:1603	CONCLUSION These results confirm the potential of these mucoadhesive vaginal tablets to enhance P4 efficacy and avoid the side effects associated with IM injection.
28956650	2	67	theme	dispersion	415:424	arg1	mixtures					378:385	mixtures	378:385	mixtures of P4/Pluronic® F-127 solid dispersion and different mucoadhesive polymers	378:460	METHODS The tablets were prepared using mixtures of P4/Pluronic® F-127 solid dispersion and different mucoadhesive polymers.
28956650	10	68	theme	mucoadhesive	1497:1508	arg1	tablets					1518:1524	these mucoadhesive vaginal tablets	1491:1524	these mucoadhesive vaginal tablets	1491:1524	CONCLUSION These results confirm the potential of these mucoadhesive vaginal tablets to enhance P4 efficacy and avoid the side effects associated with IM injection.
28956650	2	69	theme	F-127	403:407	arg1	dispersion					415:424	P4/Pluronic® F-127 solid dispersion	390:424	P4/Pluronic® F-127 solid dispersion	390:424	METHODS The tablets were prepared using mixtures of P4/Pluronic® F-127 solid dispersion and different mucoadhesive polymers.
28956650	9	70	theme	myometrium	1372:1381	arg1	thickness					1383:1391	2-fold higher myometrium thickness	1358:1391	2-fold higher myometrium thickness	1358:1391	Further, same tablets showed 2-fold higher myometrium thickness in rabbit uterus compared with IM P4 injection.
28956650	0	71	dep	in	63:64	arg1	vitro					66:70	vitro	66:70	vitro	66:70	Mucoadhesive tablets for the vaginal delivery of progesterone: in vitro evaluation and pharmacokinetics/pharmacodynamics in female rabbits.
28956650	2	72	theme	polymers	453:460	arg1	mixtures					378:385	mixtures	378:385	mixtures of P4/Pluronic® F-127 solid dispersion and different mucoadhesive polymers	378:460	METHODS The tablets were prepared using mixtures of P4/Pluronic® F-127 solid dispersion and different mucoadhesive polymers.
28956650	5	73	theme	mucoadhesion	835:846	arg1	force					848:852	in vitro mucoadhesion force	826:852	in vitro mucoadhesion force	826:852	RESULTS The tablets had satisfactory physical properties and their swelling, in vitro mucoadhesion force and ex vivo mucoadhesion time were dependent on tablet composition.
28956650	1	74	theme	extensive	308:316	arg1	metabolism					326:335	extensive hepatic metabolism	308:335	extensive hepatic metabolism	308:335	OBJECTIVE To develop mucoadhesive tablets for the vaginal delivery of progesterone (P4) to overcome its low oral bioavailability resulting from drug hydrophobicity and extensive hepatic metabolism.
28956650	6	75	theme	swelling	930:937	arg1	index					939:943	Highest swelling index	922:943	Highest swelling index	922:943	Highest swelling index and mucoadhesion time were detected for tablets containing 20% chitosan-10% alginate mixture.
28956650	4	76	theme	pharmacokinetic	570:584	arg1	properties					606:615	P4 pharmacokinetic and pharmacodynamic properties	567:615	P4 pharmacokinetic and pharmacodynamic properties	567:615	P4 pharmacokinetic and pharmacodynamic properties were evaluated in female rabbits and compared with vaginal micronized P4 tablets and intramuscular (IM) P4 injection, respectively.
28956650	2	77	theme	different	430:438	arg1	polymers					453:460	different mucoadhesive polymers	430:460	different mucoadhesive polymers	430:460	METHODS The tablets were prepared using mixtures of P4/Pluronic® F-127 solid dispersion and different mucoadhesive polymers.
28956650	0	78	theme	progesterone	49:60	arg1	delivery					37:44	the vaginal delivery	25:44	the vaginal delivery of progesterone	25:60	Mucoadhesive tablets for the vaginal delivery of progesterone: in vitro evaluation and pharmacokinetics/pharmacodynamics in female rabbits.
28956650	4	79	theme	pharmacodynamic	590:604	arg1	properties					606:615	P4 pharmacokinetic and pharmacodynamic properties	567:615	P4 pharmacokinetic and pharmacodynamic properties	567:615	P4 pharmacokinetic and pharmacodynamic properties were evaluated in female rabbits and compared with vaginal micronized P4 tablets and intramuscular (IM) P4 injection, respectively.
28956650	10	80	theme	side	1563:1566	arg1	effects					1568:1574	the side effects	1559:1574	the side effects associated with IM injection	1559:1603	CONCLUSION These results confirm the potential of these mucoadhesive vaginal tablets to enhance P4 efficacy and avoid the side effects associated with IM injection.
28956650	3	81	theme	release	534:540	arg1	properties					484:493	The tablets physical properties	463:493	The tablets physical properties	463:493	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	3	81	theme	release	534:540	arg1	kinetics					542:549	drug release kinetics	529:549	drug release kinetics	529:549	The tablets physical properties, swelling index, mucoadhesion and drug release kinetics were evaluated.
28956650	4	82	theme	vaginal	668:674	arg1	tablets					690:696	vaginal micronized P4 tablets	668:696	vaginal micronized P4 tablets	668:696	P4 pharmacokinetic and pharmacodynamic properties were evaluated in female rabbits and compared with vaginal micronized P4 tablets and intramuscular (IM) P4 injection, respectively.
28956650	9	83	theme	P4	1427:1428	arg1	injection					1430:1438	IM P4 injection	1424:1438	IM P4 injection	1424:1438	Further, same tablets showed 2-fold higher myometrium thickness in rabbit uterus compared with IM P4 injection.
28956650	8	84	theme	chitosan-alginate	1172:1188	arg1	tablets					1203:1209	chitosan-alginate mucoadhesive tablets	1172:1209	chitosan-alginate mucoadhesive tablets	1172:1209	In vivo study showed that chitosan-alginate mucoadhesive tablets had ∼2-fold higher P4 mean residence time (MRT) in the blood and 5-fold higher bioavailability compared with oral P4.
28956650	0	85	dep	tablets	13:19	arg1	pharmacokinetics/pharmacodynamics					87:119	pharmacokinetics/pharmacodynamics	87:119	pharmacokinetics/pharmacodynamics in female rabbits	87:137	Mucoadhesive tablets for the vaginal delivery of progesterone: in vitro evaluation and pharmacokinetics/pharmacodynamics in female rabbits.
28956650	0	85	dep	tablets	13:19	arg1	evaluation					72:81	in vitro evaluation	63:81	in vitro evaluation	63:81	Mucoadhesive tablets for the vaginal delivery of progesterone: in vitro evaluation and pharmacokinetics/pharmacodynamics in female rabbits.
28956650	4	86	theme	P4	687:688	arg1	tablets					690:696	vaginal micronized P4 tablets	668:696	vaginal micronized P4 tablets	668:696	P4 pharmacokinetic and pharmacodynamic properties were evaluated in female rabbits and compared with vaginal micronized P4 tablets and intramuscular (IM) P4 injection, respectively.
28956650	10	87	theme	IM	1592:1593	arg1	injection					1595:1603	IM injection	1592:1603	IM injection	1592:1603	CONCLUSION These results confirm the potential of these mucoadhesive vaginal tablets to enhance P4 efficacy and avoid the side effects associated with IM injection.
28956650	4	88	theme	micronized	676:685	arg1	tablets					690:696	vaginal micronized P4 tablets	668:696	vaginal micronized P4 tablets	668:696	P4 pharmacokinetic and pharmacodynamic properties were evaluated in female rabbits and compared with vaginal micronized P4 tablets and intramuscular (IM) P4 injection, respectively.
28956650	1	89	theme	low	244:246	arg1	bioavailability					253:267	its low oral bioavailability	240:267	its low oral bioavailability resulting from drug hydrophobicity and extensive hepatic metabolism	240:335	OBJECTIVE To develop mucoadhesive tablets for the vaginal delivery of progesterone (P4) to overcome its low oral bioavailability resulting from drug hydrophobicity and extensive hepatic metabolism.
25814035	4	0	theme	sustained	780:788	arg1	behaviour					798:806	sustained release behaviour	780:806	sustained release behaviour (important to promote bone regeneration)	780:847	The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration).
25814035	2	1	theme	porous	375:380	arg1	scaffolds					382:390	three dimensional designed porous scaffolds	348:390	three dimensional designed porous scaffolds of apatite/agarose composite	348:419	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	4	2	theme	rapid	645:649	arg1	delivery					651:658	a very rapid delivery	638:658	a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation)	638:735	The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration).
25814035	5	3	theme	ibuprofen	856:864	arg1	release					866:872	ibuprofen release	856:872	ibuprofen release	856:872	Since ibuprofen release was faster than desired, this drug was encapsulated in chitosan spheres which were then incorporated into the scaffolds, obtaining a release profile suitable for clinical application.
25814035	2	4	theme	designed	366:373	arg1	scaffolds					382:390	three dimensional designed porous scaffolds	348:390	three dimensional designed porous scaffolds of apatite/agarose composite	348:419	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	6	5	theme	controlled	1175:1184	arg1	way					1186:1188	a controlled way	1173:1188	a controlled way	1173:1188	The results obtained open the possibility to simultaneously incorporate two or more drugs to an osseous implant in a controlled way improving it for bone healing application.
25814035	4	6	theme	bone	830:833	arg1	regeneration					835:846	bone regeneration	830:846	bone regeneration	830:846	The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration).
25814035	6	7	theme	open	1079:1082	arg1	possibility					1088:1098	open the possibility to simultaneously incorporate two or more drugs to an osseous implant in a controlled way improving it for bone healing application	1079:1230	open the possibility to simultaneously incorporate two or more drugs to an osseous implant in a controlled way improving it for bone healing application	1079:1230	The results obtained open the possibility to simultaneously incorporate two or more drugs to an osseous implant in a controlled way improving it for bone healing application.
25814035	1	8	theme	dual-drug	153:161	arg1	systems					172:178	dual-drug delivery systems	153:178	dual-drug delivery systems	153:178	This work presents the tuning of drug-loaded scaffolds for bone regeneration as dual-drug delivery systems.
25814035	1	8	theme	dual-drug	153:161	arg1	tuning					96:101	the tuning	92:101	the tuning of drug-loaded scaffolds for bone regeneration	92:148	This work presents the tuning of drug-loaded scaffolds for bone regeneration as dual-drug delivery systems.
25814035	5	9	theme	release	1007:1013	arg1	profile					1015:1021	a release profile	1005:1021	a release profile suitable for clinical application	1005:1055	Since ibuprofen release was faster than desired, this drug was encapsulated in chitosan spheres which were then incorporated into the scaffolds, obtaining a release profile suitable for clinical application.
25814035	2	10	theme	dimensional	354:364	arg1	scaffolds					382:390	three dimensional designed porous scaffolds	348:390	three dimensional designed porous scaffolds of apatite/agarose composite	348:419	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	6	11	from	implant	1162:1168	arg1	way					1186:1188	a controlled way	1173:1188	a controlled way	1173:1188	The results obtained open the possibility to simultaneously incorporate two or more drugs to an osseous implant in a controlled way improving it for bone healing application.
25814035	6	12	theme	osseous	1154:1160	arg1	implant					1162:1168	an osseous implant	1151:1168	an osseous implant in a controlled way	1151:1188	The results obtained open the possibility to simultaneously incorporate two or more drugs to an osseous implant in a controlled way improving it for bone healing application.
25814035	0	13	from	scaffolds	40:48	arg1	release					17:23	release	17:23	release from composite scaffolds for bone regeneration	17:70	Tuning dual-drug release from composite scaffolds for bone regeneration.
25814035	2	14	theme	controlled	325:334	arg1	manner					336:341	a controlled manner	323:341	a controlled manner	323:341	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	4	15	dep	show	633:636	arg1	whereas					738:744	whereas	738:744	whereas	738:744	The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration).
25814035	2	16	theme	composite	411:419	arg1	scaffolds					382:390	three dimensional designed porous scaffolds	348:390	three dimensional designed porous scaffolds of apatite/agarose composite	348:419	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	5	17	theme	suitable	1023:1030	arg1	profile					1015:1021	a release profile	1005:1021	a release profile suitable for clinical application	1005:1055	Since ibuprofen release was faster than desired, this drug was encapsulated in chitosan spheres which were then incorporated into the scaffolds, obtaining a release profile suitable for clinical application.
25814035	2	18	theme	apatite/agarose	395:409	arg1	composite					411:419	apatite/agarose composite	395:419	apatite/agarose composite	395:419	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	4	19	dep	behaviour	798:806	arg1	important					809:817	important	809:817	important	809:817	The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration).
25814035	5	20	theme	clinical	1036:1043	arg1	application					1045:1055	clinical application	1036:1055	clinical application	1036:1055	Since ibuprofen release was faster than desired, this drug was encapsulated in chitosan spheres which were then incorporated into the scaffolds, obtaining a release profile suitable for clinical application.
25814035	2	21	theme	therapeutic	185:195	arg1	ibuprofen					254:262	ibuprofen	254:262	ibuprofen (anti-inflammatory drug)	254:287	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	2	21	theme	therapeutic	185:195	arg1	substances					197:206	Two therapeutic substances	181:206	Two therapeutic substances	181:206	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	2	21	theme	therapeutic	185:195	arg1	acid					220:223	zoledronic acid	209:223	zoledronic acid (anti-osteoporotic drug)	209:248	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	1	22	theme	delivery	163:170	arg1	systems					172:178	dual-drug delivery systems	153:178	dual-drug delivery systems	153:178	This work presents the tuning of drug-loaded scaffolds for bone regeneration as dual-drug delivery systems.
25814035	1	22	theme	delivery	163:170	arg1	tuning					96:101	the tuning	92:101	the tuning of drug-loaded scaffolds for bone regeneration	92:148	This work presents the tuning of drug-loaded scaffolds for bone regeneration as dual-drug delivery systems.
25814035	3	23	theme	chromatography	448:461	arg1	method					463:468	A high-performance liquid chromatography method	422:468	A high-performance liquid chromatography method	422:468	A high-performance liquid chromatography method was optimized to separate and simultaneously quantify the two drugs released from the dual-drug codelivery system.
25814035	4	24	theme	release	790:796	arg1	behaviour					798:806	sustained release behaviour	780:806	sustained release behaviour (important to promote bone regeneration)	780:847	The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration).
25814035	6	25	theme	bone	1207:1210	arg1	healing					1212:1218	bone healing	1207:1218	bone healing application	1207:1230	The results obtained open the possibility to simultaneously incorporate two or more drugs to an osseous implant in a controlled way improving it for bone healing application.
25814035	0	26	theme	composite	30:38	arg1	scaffolds					40:48	composite scaffolds	30:48	composite scaffolds for bone regeneration	30:70	Tuning dual-drug release from composite scaffolds for bone regeneration.
25814035	5	27	theme	chitosan	929:936	arg1	spheres					938:944	chitosan spheres	929:944	chitosan spheres which were then incorporated into the scaffolds	929:992	Since ibuprofen release was faster than desired, this drug was encapsulated in chitosan spheres which were then incorporated into the scaffolds, obtaining a release profile suitable for clinical application.
25814035	0	28	theme	bone	54:57	arg1	regeneration					59:70	bone regeneration	54:70	bone regeneration	54:70	Tuning dual-drug release from composite scaffolds for bone regeneration.
25814035	2	29	theme	anti-osteoporotic	226:242	arg1	drug					244:247	anti-osteoporotic drug	226:247	anti-osteoporotic drug	226:247	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	2	29	theme	anti-osteoporotic	226:242	arg1	acid					220:223	zoledronic acid	209:223	zoledronic acid (anti-osteoporotic drug)	209:248	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	1	30	theme	drug-loaded	106:116	arg1	scaffolds					118:126	drug-loaded scaffolds	106:126	drug-loaded scaffolds	106:126	This work presents the tuning of drug-loaded scaffolds for bone regeneration as dual-drug delivery systems.
25814035	3	31	theme	liquid	441:446	arg1	chromatography					448:461	A high-performance liquid chromatography	422:461	A high-performance liquid chromatography method	422:468	A high-performance liquid chromatography method was optimized to separate and simultaneously quantify the two drugs released from the dual-drug codelivery system.
25814035	4	32	theme	porous	605:610	arg1	scaffolds					612:620	The multifunctional porous scaffolds	585:620	The multifunctional porous scaffolds fabricated	585:631	The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration).
25814035	3	33	theme	dual-drug	556:564	arg1	system					577:582	the dual-drug codelivery system	552:582	the dual-drug codelivery system	552:582	A high-performance liquid chromatography method was optimized to separate and simultaneously quantify the two drugs released from the dual-drug codelivery system.
25814035	1	34	theme	scaffolds	118:126	arg1	systems					172:178	dual-drug delivery systems	153:178	dual-drug delivery systems	153:178	This work presents the tuning of drug-loaded scaffolds for bone regeneration as dual-drug delivery systems.
25814035	1	34	theme	scaffolds	118:126	arg1	tuning					96:101	the tuning	92:101	the tuning of drug-loaded scaffolds for bone regeneration	92:148	This work presents the tuning of drug-loaded scaffolds for bone regeneration as dual-drug delivery systems.
25814035	4	35	theme	multifunctional	589:603	arg1	scaffolds					612:620	The multifunctional porous scaffolds	585:620	The multifunctional porous scaffolds fabricated	585:631	The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration).
25814035	2	36	theme	zoledronic	209:218	arg1	drug					244:247	anti-osteoporotic drug	226:247	anti-osteoporotic drug	226:247	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	2	36	theme	zoledronic	209:218	arg1	substances					197:206	Two therapeutic substances	181:206	Two therapeutic substances	181:206	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	2	36	theme	zoledronic	209:218	arg1	acid					220:223	zoledronic acid	209:223	zoledronic acid (anti-osteoporotic drug)	209:248	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	2	37	theme	anti-inflammatory	265:281	arg1	ibuprofen					254:262	ibuprofen	254:262	ibuprofen (anti-inflammatory drug)	254:287	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	2	37	theme	anti-inflammatory	265:281	arg1	drug					283:286	anti-inflammatory drug	265:286	anti-inflammatory drug	265:286	Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (anti-inflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite.
25814035	1	38	theme	bone	132:135	arg1	regeneration					137:148	bone regeneration	132:148	bone regeneration	132:148	This work presents the tuning of drug-loaded scaffolds for bone regeneration as dual-drug delivery systems.
25814035	6	39	theme	healing	1212:1218	arg1	application					1220:1230	bone healing application	1207:1230	bone healing application	1207:1230	The results obtained open the possibility to simultaneously incorporate two or more drugs to an osseous implant in a controlled way improving it for bone healing application.
25814035	4	40	theme	anti-inflammatory	663:679	arg1	delivery					651:658	a very rapid delivery	638:658	a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation)	638:735	The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration).
25814035	4	41	dep	anti-inflammatory	663:679	arg1	interesting					682:692	interesting	682:692	interesting	682:692	The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration).
25814035	3	42	attach	released	538:545	arg2	drugs					532:536	the two drugs	524:536	the two drugs released from the dual-drug codelivery system	524:582	A high-performance liquid chromatography method was optimized to separate and simultaneously quantify the two drugs released from the dual-drug codelivery system.
25814035	3	42	attach	released	538:545	arg1	system					577:582	the dual-drug codelivery system	552:582	the dual-drug codelivery system	552:582	A high-performance liquid chromatography method was optimized to separate and simultaneously quantify the two drugs released from the dual-drug codelivery system.
25814035	3	43	theme	codelivery	566:575	arg1	system					577:582	the dual-drug codelivery system	552:582	the dual-drug codelivery system	552:582	A high-performance liquid chromatography method was optimized to separate and simultaneously quantify the two drugs released from the dual-drug codelivery system.
25814035	3	44	theme	high-performance	424:439	arg1	chromatography					448:461	A high-performance liquid chromatography	422:461	A high-performance liquid chromatography method	422:468	A high-performance liquid chromatography method was optimized to separate and simultaneously quantify the two drugs released from the dual-drug codelivery system.
25814035	4	45	theme	anti-osteoporotic	750:766	arg1	drug					768:771	the anti-osteoporotic drug	746:771	the anti-osteoporotic drug	746:771	The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the anti-osteoporotic drug showed sustained release behaviour (important to promote bone regeneration).
27316771	5	0	theme	resulting	655:663	arg1	fibres					688:693	The resulting CDA/chitosan composite fibres	651:693	The resulting CDA/chitosan composite fibres	651:693	The resulting CDA/chitosan composite fibres were characterized at various steps.
27316771	2	1	used	used	296:299	arg2	biowaste					282:289	an easily available biowaste	262:289	an easily available biowaste	262:289	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	1	used	used	296:299	arg2	source					304:309	source	304:309	source to extract nanocellulose fibres (CNFs)	304:348	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	1	used	used	296:299	arg2	Bagasse					253:259	Bagasse	253:259	Bagasse	253:259	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	0	2	theme	antimicrobial	86:98	arg1	membranes					100:108	Schiff-base based antimicrobial membranes	68:108	Schiff-base based antimicrobial membranes	68:108	Extraction and functionalization of bagasse cellulose nanofibres to Schiff-base based antimicrobial membranes.
27316771	5	3	theme	CDA/chitosan	665:676	arg1	fibres					688:693	The resulting CDA/chitosan composite fibres	651:693	The resulting CDA/chitosan composite fibres	651:693	The resulting CDA/chitosan composite fibres were characterized at various steps.
27316771	3	4	theme	nanocellulose	528:540	arg1	CDA					554:556	CDA	554:556	CDA	554:556	The CNFs were subjected to periodate oxidation to obtain nanocellulose dialdehyde (CDA).
27316771	3	4	theme	nanocellulose	528:540	arg1	dialdehyde					542:551	nanocellulose dialdehyde	528:551	nanocellulose dialdehyde (CDA)	528:557	The CNFs were subjected to periodate oxidation to obtain nanocellulose dialdehyde (CDA).
27316771	8	5	theme	excellent	870:878	arg1	properties					894:903	excellent antimicrobial properties	870:903	excellent antimicrobial properties	870:903	The composite films show excellent antimicrobial properties when tested against Staphylococcus aureus and Escherichia coli.
27316771	9	6	theme	antimicrobial	974:986	arg1	films					988:992	Such antimicrobial films	969:992	Such antimicrobial films	969:992	Such antimicrobial films have potential applications in the formation of antimicrobial packaging material.
27316771	5	7	theme	composite	678:686	arg1	fibres					688:693	The resulting CDA/chitosan composite fibres	651:693	The resulting CDA/chitosan composite fibres	651:693	The resulting CDA/chitosan composite fibres were characterized at various steps.
27316771	7	8	contain	have	816:819	arg1	films					810:814	The films	806:814	The films	806:814	The films have good physical strength.
27316771	7	8	contain	have	816:819	arg2	strength					835:842	good physical strength	821:842	good physical strength	821:842	The films have good physical strength.
27316771	4	9	theme	amino	605:609	arg1	chitosan					621:628	chitosan	621:628	chitosan	621:628	The aldehyde groups of CDA were reacted with amino groups of chitosan to form Schiff-base.
27316771	4	9	theme	amino	605:609	arg1	groups					611:616	amino groups	605:616	amino groups of chitosan	605:628	The aldehyde groups of CDA were reacted with amino groups of chitosan to form Schiff-base.
27316771	8	10	theme	composite	849:857	arg1	films					859:863	The composite films	845:863	The composite films	845:863	The composite films show excellent antimicrobial properties when tested against Staphylococcus aureus and Escherichia coli.
27316771	8	11	theme	antimicrobial	880:892	arg1	properties					894:903	excellent antimicrobial properties	870:903	excellent antimicrobial properties	870:903	The composite films show excellent antimicrobial properties when tested against Staphylococcus aureus and Escherichia coli.
27316771	1	12	theme	composite	190:198	arg1	modification					216:227	its further modification	204:227	its further modification to antimicrobial films	204:250	The work reported in this paper involves synthesis of a nanocellulose/chitosan composite and its further modification to antimicrobial films.
27316771	1	12	theme	composite	190:198	arg1	synthesis					152:160	synthesis	152:160	synthesis of a nanocellulose/chitosan composite	152:198	The work reported in this paper involves synthesis of a nanocellulose/chitosan composite and its further modification to antimicrobial films.
27316771	9	13	theme	potential	999:1007	arg1	applications					1009:1020	potential applications	999:1020	potential applications	999:1020	Such antimicrobial films have potential applications in the formation of antimicrobial packaging material.
27316771	9	14	theme	Such	969:972	arg1	films					988:992	Such antimicrobial films	969:992	Such antimicrobial films	969:992	Such antimicrobial films have potential applications in the formation of antimicrobial packaging material.
27316771	2	15	theme	chemical	385:392	arg1	treatments					394:403	mechanical and chemical treatments	370:403	mechanical and chemical treatments including alkaline steam explosion and high shear homogenization	370:468	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	15	theme	chemical	385:392	arg1	homogenization					455:468	high shear homogenization	444:468	high shear homogenization	444:468	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	15	theme	chemical	385:392	arg1	explosion					430:438	alkaline steam explosion	415:438	alkaline steam explosion	415:438	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	16	theme	nanocellulose	322:334	arg1	CNFs					344:347	CNFs	344:347	CNFs	344:347	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	16	theme	nanocellulose	322:334	arg1	fibres					336:341	extract nanocellulose fibres	314:341	extract nanocellulose fibres (CNFs)	314:348	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	1	17	theme	further	208:214	arg1	modification					216:227	its further modification	204:227	its further modification to antimicrobial films	204:250	The work reported in this paper involves synthesis of a nanocellulose/chitosan composite and its further modification to antimicrobial films.
27316771	2	18	theme	shear	449:453	arg1	homogenization					455:468	high shear homogenization	444:468	high shear homogenization	444:468	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	4	19	theme	aldehyde	564:571	arg1	groups					573:578	The aldehyde groups	560:578	The aldehyde groups of CDA	560:585	The aldehyde groups of CDA were reacted with amino groups of chitosan to form Schiff-base.
27316771	4	19	theme	aldehyde	564:571	arg1	CDA					583:585	CDA	583:585	CDA	583:585	The aldehyde groups of CDA were reacted with amino groups of chitosan to form Schiff-base.
27316771	2	20	theme	extract	314:320	arg1	CNFs					344:347	CNFs	344:347	CNFs	344:347	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	20	theme	extract	314:320	arg1	fibres					336:341	extract nanocellulose fibres	314:341	extract nanocellulose fibres (CNFs)	314:348	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	21	theme	high	444:447	arg1	homogenization					455:468	high shear homogenization	444:468	high shear homogenization	444:468	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	4	22	theme	CDA	583:585	arg1	groups					573:578	The aldehyde groups	560:578	The aldehyde groups of CDA	560:585	The aldehyde groups of CDA were reacted with amino groups of chitosan to form Schiff-base.
27316771	4	22	theme	CDA	583:585	arg1	CDA					583:585	CDA	583:585	CDA	583:585	The aldehyde groups of CDA were reacted with amino groups of chitosan to form Schiff-base.
27316771	1	23	theme	nanocellulose/chitosan	167:188	arg1	composite					190:198	a nanocellulose/chitosan composite	165:198	a nanocellulose/chitosan composite	165:198	The work reported in this paper involves synthesis of a nanocellulose/chitosan composite and its further modification to antimicrobial films.
27316771	7	24	theme	physical	826:833	arg1	strength					835:842	good physical strength	821:842	good physical strength	821:842	The films have good physical strength.
27316771	0	25	theme	cellulose	44:52	arg1	functionalization					15:31	functionalization	15:31	functionalization	15:31	Extraction and functionalization of bagasse cellulose nanofibres to Schiff-base based antimicrobial membranes.
27316771	0	25	theme	cellulose	44:52	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and functionalization of bagasse cellulose nanofibres to Schiff-base based antimicrobial membranes.
27316771	1	26	theme	antimicrobial	232:244	arg1	films					246:250	antimicrobial films	232:250	antimicrobial films	232:250	The work reported in this paper involves synthesis of a nanocellulose/chitosan composite and its further modification to antimicrobial films.
27316771	2	27	theme	steam	424:428	arg1	explosion					430:438	alkaline steam explosion	415:438	alkaline steam explosion	415:438	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	0	28	theme	bagasse	36:42	arg1	cellulose					44:52	bagasse cellulose	36:52	bagasse cellulose	36:52	Extraction and functionalization of bagasse cellulose nanofibres to Schiff-base based antimicrobial membranes.
27316771	9	29	theme	antimicrobial	1042:1054	arg1	material					1066:1073	antimicrobial packaging material	1042:1073	antimicrobial packaging material	1042:1073	Such antimicrobial films have potential applications in the formation of antimicrobial packaging material.
27316771	9	30	contain	have	994:997	arg1	films					988:992	Such antimicrobial films	969:992	Such antimicrobial films	969:992	Such antimicrobial films have potential applications in the formation of antimicrobial packaging material.
27316771	9	30	contain	have	994:997	arg2	applications					1009:1020	potential applications	999:1020	potential applications	999:1020	Such antimicrobial films have potential applications in the formation of antimicrobial packaging material.
27316771	2	31	theme	alkaline	415:422	arg1	explosion					430:438	alkaline steam explosion	415:438	alkaline steam explosion	415:438	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	9	32	theme	packaging	1056:1064	arg1	material					1066:1073	antimicrobial packaging material	1042:1073	antimicrobial packaging material	1042:1073	Such antimicrobial films have potential applications in the formation of antimicrobial packaging material.
27316771	7	33	theme	good	821:824	arg1	strength					835:842	good physical strength	821:842	good physical strength	821:842	The films have good physical strength.
27316771	6	34	theme	cellulose	775:783	arg1	binder					798:803	a binder	796:803	a binder	796:803	The fibres were then cast into films using cellulose acetate as a binder.
27316771	6	34	theme	cellulose	775:783	arg1	acetate					785:791	cellulose acetate	775:791	cellulose acetate	775:791	The fibres were then cast into films using cellulose acetate as a binder.
27316771	9	35	theme	material	1066:1073	arg1	formation					1029:1037	the formation	1025:1037	the formation of antimicrobial packaging material	1025:1073	Such antimicrobial films have potential applications in the formation of antimicrobial packaging material.
27316771	5	36	theme	various	717:723	arg1	steps					725:729	various steps	717:729	various steps	717:729	The resulting CDA/chitosan composite fibres were characterized at various steps.
27316771	3	37	theme	periodate	498:506	arg1	oxidation					508:516	periodate oxidation	498:516	periodate oxidation	498:516	The CNFs were subjected to periodate oxidation to obtain nanocellulose dialdehyde (CDA).
27316771	0	38	theme	based	80:84	arg1	membranes					100:108	Schiff-base based antimicrobial membranes	68:108	Schiff-base based antimicrobial membranes	68:108	Extraction and functionalization of bagasse cellulose nanofibres to Schiff-base based antimicrobial membranes.
27316771	2	39	theme	available	272:280	arg1	biowaste					282:289	an easily available biowaste	262:289	an easily available biowaste	262:289	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	39	theme	available	272:280	arg1	Bagasse					253:259	Bagasse	253:259	Bagasse	253:259	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	40	theme	mechanical	370:379	arg1	treatments					394:403	mechanical and chemical treatments	370:403	mechanical and chemical treatments including alkaline steam explosion and high shear homogenization	370:468	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	40	theme	mechanical	370:379	arg1	homogenization					455:468	high shear homogenization	444:468	high shear homogenization	444:468	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	2	40	theme	mechanical	370:379	arg1	explosion					430:438	alkaline steam explosion	415:438	alkaline steam explosion	415:438	Bagasse, an easily available biowaste, was used as source to extract nanocellulose fibres (CNFs) by subjecting it to mechanical and chemical treatments including alkaline steam explosion and high shear homogenization.
27316771	0	41	theme	Schiff-base	68:78	arg1	membranes					100:108	Schiff-base based antimicrobial membranes	68:108	Schiff-base based antimicrobial membranes	68:108	Extraction and functionalization of bagasse cellulose nanofibres to Schiff-base based antimicrobial membranes.
27316771	4	42	theme	chitosan	621:628	arg1	chitosan					621:628	chitosan	621:628	chitosan	621:628	The aldehyde groups of CDA were reacted with amino groups of chitosan to form Schiff-base.
27316771	4	42	theme	chitosan	621:628	arg1	groups					611:616	amino groups	605:616	amino groups of chitosan	605:628	The aldehyde groups of CDA were reacted with amino groups of chitosan to form Schiff-base.
26258851	7	0	theme	Histological	1098:1109	arg1	analysis					1111:1118	Histological analysis	1098:1118	Histological analysis	1098:1118	Histological analysis revealed that de novo bone and collagen formation in the pores of the scaffolds gradually increased from 2 weeks post-implantation in both groups and that the scaffold gradually degraded as bone formation proceeded.
26258851	8	1	theme	same	1478:1481	arg1	points					1488:1493	the same time points	1474:1493	the same time points (P < 0.05)	1474:1504	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	8	1	theme	same	1478:1481	arg1	<					1498:1498	P < 0.05	1496:1503	P < 0.05	1496:1503	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	3	2	theme	femoral	528:534	arg1	intermusculare					544:557	the femoral spatium intermusculare	524:557	the femoral spatium intermusculare of SD rats	524:568	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	9	3	with	par	1749:1751	arg1	formation					1763:1771	bone formation	1758:1771	bone formation	1758:1771	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	9	4	theme	promising	1563:1571	arg1	engineering					1585:1595	promising bone tissue engineering substitutes	1563:1607	promising bone tissue engineering substitutes	1563:1607	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	9	4	theme	promising	1563:1571	arg1	composites					1548:1557	nHA-CS osteo-induced BMSC composites	1522:1557	nHA-CS osteo-induced BMSC composites	1522:1557	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	0	5	from	osteogenesis	8:19	arg1	model					90:94	a rat model	84:94	a rat model	84:94	Ectopic osteogenesis and scaffold biodegradation of nano-hydroxyapatite-chitosan in a rat model.
26258851	8	6	theme	collagen	1367:1374	arg1	formation					1376:1384	de novo bone and collagen formation	1350:1384	formation	1376:1384	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	3	7	theme	spatium	536:542	arg1	intermusculare					544:557	the femoral spatium intermusculare	524:557	the femoral spatium intermusculare of SD rats	524:568	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	9	8	theme	nHA-CS	1729:1734	arg1	scaffolds					1736:1744	nHA-CS scaffolds	1729:1744	nHA-CS scaffolds on par with bone formation	1729:1771	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	8	9	theme	P	1496:1496	arg1	points					1488:1493	the same time points	1474:1493	the same time points (P < 0.05)	1474:1504	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	8	9	theme	P	1496:1496	arg1	<					1498:1498	P < 0.05	1496:1503	P < 0.05	1496:1503	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	8	10	theme	scaffold	1390:1397	arg1	degradation					1399:1409	scaffold degradation	1390:1409	scaffold degradation	1390:1409	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	2	11	theme	scaffold	377:384	arg1	biodegradation					386:399	scaffold biodegradation	377:399	scaffold biodegradation	377:399	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	6	12	theme	P	1087:1087	arg1	points					1079:1084	the same time points	1065:1084	the same time points (P < 0.05)	1065:1095	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	6	12	theme	P	1087:1087	arg1	<					1089:1089	P < 0.05	1087:1094	P < 0.05	1087:1094	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	5	13	theme	area	896:899	arg1	percentages					876:886	the percentages	872:886	the percentages of bone area, scaffold area and collagen area	872:932	The implants were then harvested and subjected to hematoxylin and eosin (HE) and Masson's trichrome staining, and the percentages of bone area, scaffold area and collagen area were compared between the two groups.
26258851	7	14	theme	collagen	1151:1158	arg1	formation					1160:1168	de novo bone and collagen formation	1134:1168	formation	1160:1168	Histological analysis revealed that de novo bone and collagen formation in the pores of the scaffolds gradually increased from 2 weeks post-implantation in both groups and that the scaffold gradually degraded as bone formation proceeded.
26258851	5	15	theme	scaffold	902:909	arg1	area					911:914	scaffold area	902:914	scaffold area	902:914	The implants were then harvested and subjected to hematoxylin and eosin (HE) and Masson's trichrome staining, and the percentages of bone area, scaffold area and collagen area were compared between the two groups.
26258851	7	16	from	bone	1142:1145	arg1	pores					1177:1181	the pores	1173:1181	the pores of the scaffolds	1173:1198	Histological analysis revealed that de novo bone and collagen formation in the pores of the scaffolds gradually increased from 2 weeks post-implantation in both groups and that the scaffold gradually degraded as bone formation proceeded.
26258851	2	17	theme	composites	307:316	arg1	ability					234:240	the osteogenic ability	219:240	the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites	219:316	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	9	18	theme	osteogenic	1664:1673	arg1	ability					1675:1681	the osteogenic ability	1660:1681	the osteogenic ability	1660:1681	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	9	19	theme	nHA-CS	1522:1527	arg1	engineering					1585:1595	promising bone tissue engineering substitutes	1563:1607	promising bone tissue engineering substitutes	1563:1607	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	9	19	theme	nHA-CS	1522:1527	arg1	composites					1548:1557	nHA-CS osteo-induced BMSC composites	1522:1557	nHA-CS osteo-induced BMSC composites	1522:1557	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	7	20	theme	scaffolds	1190:1198	arg1	pores					1177:1181	the pores	1173:1181	the pores of the scaffolds	1173:1198	Histological analysis revealed that de novo bone and collagen formation in the pores of the scaffolds gradually increased from 2 weeks post-implantation in both groups and that the scaffold gradually degraded as bone formation proceeded.
26258851	3	21	theme	SD	562:563	arg1	rats					565:568	SD rats	562:568	SD rats	562:568	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	9	22	theme	active	1695:1700	arg1	role					1702:1705	an active role	1692:1705	an active role	1692:1705	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	3	23	theme	nHA-CS	472:477	arg1	scaffolds					479:487	the nHA-CS scaffolds	468:487	the nHA-CS scaffolds (nHA-CS group)	468:502	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	3	23	theme	nHA-CS	472:477	arg1	group					497:501	nHA-CS group	490:501	nHA-CS group	490:501	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	3	24	theme	nHA-CS+cells	444:455	arg1	composites					432:441	The nHA-CS osteo-induced BMSC composites	402:441	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group)	402:462	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	3	24	theme	nHA-CS+cells	444:455	arg1	group					457:461	nHA-CS+cells group	444:461	nHA-CS+cells group	444:461	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	5	25	theme	trichrome	848:856	arg1	staining					858:865	Masson's trichrome staining	839:865	Masson's trichrome staining	839:865	The implants were then harvested and subjected to hematoxylin and eosin (HE) and Masson's trichrome staining, and the percentages of bone area, scaffold area and collagen area were compared between the two groups.
26258851	9	26	theme	osteo-induced	1529:1541	arg1	engineering					1585:1595	promising bone tissue engineering substitutes	1563:1607	promising bone tissue engineering substitutes	1563:1607	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	9	26	theme	osteo-induced	1529:1541	arg1	composites					1548:1557	nHA-CS osteo-induced BMSC composites	1522:1557	nHA-CS osteo-induced BMSC composites	1522:1557	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	3	27	theme	rats	565:568	arg1	intermusculare					544:557	the femoral spatium intermusculare	524:557	the femoral spatium intermusculare of SD rats	524:568	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	6	28	theme	implants	993:1000	arg1	higher					1007:1012	higher	1007:1012	higher	1007:1012	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	6	28	theme	implants	993:1000	arg1	values					979:984	The CT values	972:984	The CT values of the implants	972:1000	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	3	29	theme	BMSC	427:430	arg1	composites					432:441	The nHA-CS osteo-induced BMSC composites	402:441	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group)	402:462	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	3	29	theme	BMSC	427:430	arg1	group					457:461	nHA-CS+cells group	444:461	nHA-CS+cells group	444:461	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	0	30	from	biodegradation	34:47	arg1	model					90:94	a rat model	84:94	a rat model	84:94	Ectopic osteogenesis and scaffold biodegradation of nano-hydroxyapatite-chitosan in a rat model.
26258851	9	31	theme	BMSC	1543:1546	arg1	engineering					1585:1595	promising bone tissue engineering substitutes	1563:1607	promising bone tissue engineering substitutes	1563:1607	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	9	31	theme	BMSC	1543:1546	arg1	composites					1548:1557	nHA-CS osteo-induced BMSC composites	1522:1557	nHA-CS osteo-induced BMSC composites	1522:1557	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	7	32	from	formation	1160:1168	arg1	pores					1177:1181	the pores	1173:1181	the pores of the scaffolds	1173:1198	Histological analysis revealed that de novo bone and collagen formation in the pores of the scaffolds gradually increased from 2 weeks post-implantation in both groups and that the scaffold gradually degraded as bone formation proceeded.
26258851	6	33	theme	same	1069:1072	arg1	points					1079:1084	the same time points	1065:1084	the same time points (P < 0.05)	1065:1095	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	6	33	theme	same	1069:1072	arg1	<					1089:1089	P < 0.05	1087:1094	P < 0.05	1087:1094	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	4	34	theme	computerized	652:663	arg1	CT					677:678	CT	677:678	CT	677:678	At 2, 4, 6, 8, and 12 weeks post-implantation, the rat femurs were scanned using computerized tomography (CT), and the CT values of the implants were measured and comparatively analyzed.
26258851	4	34	theme	computerized	652:663	arg1	tomography					665:674	computerized tomography	652:674	computerized tomography (CT)	652:679	At 2, 4, 6, 8, and 12 weeks post-implantation, the rat femurs were scanned using computerized tomography (CT), and the CT values of the implants were measured and comparatively analyzed.
26258851	0	35	theme	Ectopic	0:6	arg1	osteogenesis					8:19	Ectopic osteogenesis	0:19	Ectopic osteogenesis	0:19	Ectopic osteogenesis and scaffold biodegradation of nano-hydroxyapatite-chitosan in a rat model.
26258851	1	36	theme	bone-formation	101:114	arg1	processes					144:152	The bone-formation and scaffold-biodegradation processes	97:152	The bone-formation and scaffold-biodegradation processes	97:152	The bone-formation and scaffold-biodegradation processes have not been fully characterized.
26258851	0	37	theme	scaffold	25:32	arg1	biodegradation					34:47	scaffold biodegradation	25:47	scaffold biodegradation	25:47	Ectopic osteogenesis and scaffold biodegradation of nano-hydroxyapatite-chitosan in a rat model.
26258851	7	38	theme	weeks	1227:1231	arg1	post-implantation					1233:1249	2 weeks post-implantation	1225:1249	2 weeks post-implantation	1225:1249	Histological analysis revealed that de novo bone and collagen formation in the pores of the scaffolds gradually increased from 2 weeks post-implantation in both groups and that the scaffold gradually degraded as bone formation proceeded.
26258851	9	39	from	scaffolds	1736:1744	arg1	par					1749:1751	par	1749:1751	par with bone formation	1749:1771	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	4	40	theme	implants	707:714	arg1	values					693:698	the CT values	686:698	the CT values of the implants	686:714	At 2, 4, 6, 8, and 12 weeks post-implantation, the rat femurs were scanned using computerized tomography (CT), and the CT values of the implants were measured and comparatively analyzed.
26258851	3	41	theme	osteo-induced	413:425	arg1	composites					432:441	The nHA-CS osteo-induced BMSC composites	402:441	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group)	402:462	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	3	41	theme	osteo-induced	413:425	arg1	group					457:461	nHA-CS+cells group	444:461	nHA-CS+cells group	444:461	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	9	42	dep	engineering	1585:1595	arg1	substitutes					1597:1607	substitutes	1597:1607	substitutes	1597:1607	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	5	43	theme	area	911:914	arg1	percentages					876:886	the percentages	872:886	the percentages of bone area, scaffold area and collagen area	872:932	The implants were then harvested and subjected to hematoxylin and eosin (HE) and Masson's trichrome staining, and the percentages of bone area, scaffold area and collagen area were compared between the two groups.
26258851	1	44	theme	scaffold-biodegradation	120:142	arg1	processes					144:152	The bone-formation and scaffold-biodegradation processes	97:152	The bone-formation and scaffold-biodegradation processes	97:152	The bone-formation and scaffold-biodegradation processes have not been fully characterized.
26258851	6	45	theme	CT	976:977	arg1	higher					1007:1012	higher	1007:1012	higher	1007:1012	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	6	45	theme	CT	976:977	arg1	values					979:984	The CT values	972:984	The CT values of the implants	972:1000	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	9	46	from	par	1749:1751	arg1	degradation					1714:1724	the degradation	1710:1724	the degradation of nHA-CS scaffolds on par with bone formation	1710:1771	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	9	47	theme	bone	1573:1576	arg1	engineering					1585:1595	promising bone tissue engineering substitutes	1563:1607	promising bone tissue engineering substitutes	1563:1607	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	9	47	theme	bone	1573:1576	arg1	composites					1548:1557	nHA-CS osteo-induced BMSC composites	1522:1557	nHA-CS osteo-induced BMSC composites	1522:1557	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	9	48	theme	bone	1758:1761	arg1	formation					1763:1771	bone formation	1758:1771	bone formation	1758:1771	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	2	49	theme	marrow	271:276	arg1	composites					307:316	nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites	245:316	nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites	245:316	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	2	50	dep	marrow	271:276	arg1	BMSC					301:304	BMSC	301:304	BMSC	301:304	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	2	50	dep	marrow	271:276	arg1	cell					295:298	mesenchymal stem cell	278:298	nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites	245:316	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	8	51	dep	de	1350:1351	arg1	novo					1353:1356	novo	1353:1356	novo	1353:1356	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	9	52	theme	tissue	1578:1583	arg1	engineering					1585:1595	promising bone tissue engineering substitutes	1563:1607	promising bone tissue engineering substitutes	1563:1607	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	9	52	theme	tissue	1578:1583	arg1	composites					1548:1557	nHA-CS osteo-induced BMSC composites	1522:1557	nHA-CS osteo-induced BMSC composites	1522:1557	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	8	53	theme	de	1350:1351	arg1	bone					1358:1361	de novo bone and collagen formation	1350:1384	bone	1358:1361	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	2	54	theme	bone	266:269	arg1	marrow					271:276	nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC)	245:305	nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites	245:316	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	3	55	theme	nHA-CS	490:495	arg1	scaffolds					479:487	the nHA-CS scaffolds	468:487	the nHA-CS scaffolds (nHA-CS group)	468:502	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	3	55	theme	nHA-CS	490:495	arg1	group					497:501	nHA-CS group	490:501	nHA-CS group	490:501	The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats.
26258851	9	56	from	degradation	1714:1724	arg1	par					1749:1751	par	1749:1751	par with bone formation	1749:1771	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	8	57	theme	nHA-CS	1458:1463	arg1	group					1465:1469	the nHA-CS group	1454:1469	the nHA-CS group	1454:1469	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	2	58	theme	osteo-induced	252:264	arg1	marrow					271:276	nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC)	245:305	nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites	245:316	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	8	59	theme	nHA-CS+cells	1427:1438	arg1	group					1440:1444	the nHA-CS+cells group	1423:1444	the nHA-CS+cells group	1423:1444	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	6	60	theme	nHA-CS	1049:1054	arg1	group					1056:1060	the nHA-CS group	1045:1060	the nHA-CS group	1045:1060	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	0	61	theme	nano-hydroxyapatite-chitosan	52:79	arg1	osteogenesis					8:19	Ectopic osteogenesis	0:19	Ectopic osteogenesis	0:19	Ectopic osteogenesis and scaffold biodegradation of nano-hydroxyapatite-chitosan in a rat model.
26258851	0	61	theme	nano-hydroxyapatite-chitosan	52:79	arg1	biodegradation					34:47	scaffold biodegradation	25:47	scaffold biodegradation	25:47	Ectopic osteogenesis and scaffold biodegradation of nano-hydroxyapatite-chitosan in a rat model.
26258851	2	62	theme	nHA-CS	245:250	arg1	marrow					271:276	nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC)	245:305	nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites	245:316	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	9	63	theme	osteo-induced	1614:1626	arg1	BMSCs					1628:1632	osteo-induced BMSCs	1614:1632	osteo-induced BMSCs	1614:1632	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	5	64	theme	collagen	920:927	arg1	area					929:932	collagen area	920:932	collagen area	920:932	The implants were then harvested and subjected to hematoxylin and eosin (HE) and Masson's trichrome staining, and the percentages of bone area, scaffold area and collagen area were compared between the two groups.
26258851	7	65	theme	bone	1310:1313	arg1	formation					1315:1323	bone formation	1310:1323	bone formation	1310:1323	Histological analysis revealed that de novo bone and collagen formation in the pores of the scaffolds gradually increased from 2 weeks post-implantation in both groups and that the scaffold gradually degraded as bone formation proceeded.
26258851	2	66	theme	bone	358:361	arg1	formation					363:371	bone formation	358:371	bone formation	358:371	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	5	67	theme	bone	891:894	arg1	area					896:899	bone area	891:899	bone area	891:899	The implants were then harvested and subjected to hematoxylin and eosin (HE) and Masson's trichrome staining, and the percentages of bone area, scaffold area and collagen area were compared between the two groups.
26258851	8	68	theme	time	1483:1486	arg1	points					1488:1493	the same time points	1474:1493	the same time points (P < 0.05)	1474:1504	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	8	68	theme	time	1483:1486	arg1	<					1498:1498	P < 0.05	1496:1503	P < 0.05	1496:1503	However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05).
26258851	6	69	theme	time	1074:1077	arg1	points					1079:1084	the same time points	1065:1084	the same time points (P < 0.05)	1065:1095	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	6	69	theme	time	1074:1077	arg1	<					1089:1089	P < 0.05	1087:1094	P < 0.05	1087:1094	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	2	70	theme	osteogenic	223:232	arg1	ability					234:240	the osteogenic ability	219:240	the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites	219:316	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	5	71	theme	area	929:932	arg1	percentages					876:886	the percentages	872:886	the percentages of bone area, scaffold area and collagen area	872:932	The implants were then harvested and subjected to hematoxylin and eosin (HE) and Masson's trichrome staining, and the percentages of bone area, scaffold area and collagen area were compared between the two groups.
26258851	9	72	theme	scaffolds	1736:1744	arg1	degradation					1714:1724	the degradation	1710:1724	the degradation of nHA-CS scaffolds on par with bone formation	1710:1771	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	4	73	theme	CT	690:691	arg1	values					693:698	the CT values	686:698	the CT values of the implants	686:714	At 2, 4, 6, 8, and 12 weeks post-implantation, the rat femurs were scanned using computerized tomography (CT), and the CT values of the implants were measured and comparatively analyzed.
26258851	0	74	theme	rat	86:88	arg1	model					90:94	a rat model	84:94	a rat model	84:94	Ectopic osteogenesis and scaffold biodegradation of nano-hydroxyapatite-chitosan in a rat model.
26258851	4	75	theme	rat	622:624	arg1	femurs					626:631	the rat femurs	618:631	the rat femurs	618:631	At 2, 4, 6, 8, and 12 weeks post-implantation, the rat femurs were scanned using computerized tomography (CT), and the CT values of the implants were measured and comparatively analyzed.
26258851	2	76	theme	stem	290:293	arg1	BMSC					301:304	BMSC	301:304	BMSC	301:304	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	2	76	theme	stem	290:293	arg1	cell					295:298	mesenchymal stem cell	278:298	nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites	245:316	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	6	77	theme	nHA-CS+cells	1021:1032	arg1	group					1034:1038	the nHA-CS+cells group	1017:1038	the nHA-CS+cells group	1017:1038	The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05).
26258851	7	78	dep	de	1134:1135	arg1	novo					1137:1140	novo	1137:1140	novo	1137:1140	Histological analysis revealed that de novo bone and collagen formation in the pores of the scaffolds gradually increased from 2 weeks post-implantation in both groups and that the scaffold gradually degraded as bone formation proceeded.
26258851	9	79	from	engineering	1585:1595	arg1	conclusion					1510:1519	conclusion	1510:1519	conclusion	1510:1519	In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
26258851	2	80	theme	mesenchymal	278:288	arg1	BMSC					301:304	BMSC	301:304	BMSC	301:304	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	2	80	theme	mesenchymal	278:288	arg1	cell					295:298	mesenchymal stem cell	278:298	nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites	245:316	This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation.
26258851	7	81	theme	de	1134:1135	arg1	bone					1142:1145	de novo bone and collagen formation	1134:1168	bone	1142:1145	Histological analysis revealed that de novo bone and collagen formation in the pores of the scaffolds gradually increased from 2 weeks post-implantation in both groups and that the scaffold gradually degraded as bone formation proceeded.
26258851	4	82	theme	weeks	593:597	arg1	post-implantation					599:615	2, 4, 6, 8, and 12 weeks post-implantation	574:615	2, 4, 6, 8, and 12 weeks post-implantation	574:615	At 2, 4, 6, 8, and 12 weeks post-implantation, the rat femurs were scanned using computerized tomography (CT), and the CT values of the implants were measured and comparatively analyzed.
28944711	2	0	theme	systems	349:355	arg1	fabrication					310:320	the fabrication	306:320	the fabrication of different bone scaffold systems including ceramic-polymer hybrid system	306:395	Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system.
28944711	9	1	theme	biphasic	1575:1582	arg1	granules					1602:1609	biphasic calcium phosphate granules	1575:1609	biphasic calcium phosphate granules	1575:1609	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	6	2	theme	pore	983:986	arg1	sizes					988:992	a suitable pore sizes	972:992	a suitable pore sizes	972:992	The resulting scaffold had a porosity of 56% with a suitable pore sizes.
28944711	1	3	theme	bone	268:271	arg1	characteristics					226:240	characteristics	226:240	characteristics	226:240	An ideal bone substitute should be made of biocompatible materials that mimic the structure, characteristics, and functions of natural bone.
28944711	1	3	theme	bone	268:271	arg1	functions					247:255	functions	247:255	functions	247:255	An ideal bone substitute should be made of biocompatible materials that mimic the structure, characteristics, and functions of natural bone.
28944711	1	3	theme	bone	268:271	arg1	structure					215:223	structure	215:223	structure	215:223	An ideal bone substitute should be made of biocompatible materials that mimic the structure, characteristics, and functions of natural bone.
28944711	8	4	from	scaffold	1331:1338	arg1	presence					1300:1307	the presence	1296:1307	the presence of granules in hybrid scaffold	1296:1338	Swelling and degradation rates decreased in the hybrid scaffold compared to hydrogel due to the presence of granules in hybrid scaffold.
28944711	11	5	theme	Histological	1847:1858	arg1	section					1860:1866	Histological section	1847:1866	Histological section	1847:1866	Histological section confirmed the formation of dense collagenous tissue and new bone in biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds at two months.
28944711	4	6	from	1 mm	718:721	arg1	diameter					726:733	diameter	726:733	diameter	726:733	This hybrid system is composed of ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel.
28944711	6	7	theme	%	965:965	arg1	porosity					951:958	a porosity	949:958	a porosity of 56% with a suitable pore sizes	949:992	The resulting scaffold had a porosity of 56% with a suitable pore sizes.
28944711	8	8	theme	granules	1312:1319	arg1	presence					1300:1307	the presence	1296:1307	the presence of granules in hybrid scaffold	1296:1338	Swelling and degradation rates decreased in the hybrid scaffold compared to hydrogel due to the presence of granules in hybrid scaffold.
28944711	4	9	theme	calcium	681:687	arg1	phosphate					689:697	ceramic biphasic calcium phosphate	664:697	ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel	664:787	This hybrid system is composed of ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel.
28944711	10	10	theme	better	1772:1777	arg1	formation					1784:1792	better bone formation	1772:1792	better bone formation	1772:1792	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	7	11	theme	bone	1185:1188	arg1	substitution					1190:1201	cancellous bone substitution	1174:1201	cancellous bone substitution	1174:1201	The mechanical strength of biphasic calcium phosphate granule increased after loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution.
28944711	9	12	theme	In vitro	1341:1348	arg1	studies					1368:1374	In vitro cytocompatibility studies	1341:1374	In vitro cytocompatibility studies	1341:1374	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	3	13	theme	interconnected	597:610	arg1	structure					619:627	highly interconnected porous structure	590:627	highly interconnected porous structure	590:627	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	8	14	theme	hybrid	1324:1329	arg1	scaffold					1331:1338	hybrid scaffold	1324:1338	hybrid scaffold	1324:1338	Swelling and degradation rates decreased in the hybrid scaffold compared to hydrogel due to the presence of granules in hybrid scaffold.
28944711	10	15	from	Analysis	1612:1619	arg1	samples					1702:1708	the implanted samples	1688:1708	the implanted samples	1688:1708	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	7	16	from	4.26 ± 0.43	1110:1120	arg1	acid-gelatin					1092:1103	loading hyaluronic acid-gelatin	1073:1103	loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution	1073:1201	The mechanical strength of biphasic calcium phosphate granule increased after loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution.
28944711	4	17	theme	acid-gelatin	767:778	arg1	hydrogel					780:787	hyaluronic acid-gelatin hydrogel	756:787	hyaluronic acid-gelatin hydrogel	756:787	This hybrid system is composed of ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel.
28944711	11	18	theme	calcium	1945:1951	arg1	scaffolds					1987:1995	biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds	1936:1995	biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds	1936:1995	Histological section confirmed the formation of dense collagenous tissue and new bone in biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds at two months.
28944711	9	19	theme	preosteoblasts	1393:1406	arg1	MC3T3-E1					1409:1416	MC3T3-E1	1409:1416	MC3T3-E1	1409:1416	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	9	19	theme	preosteoblasts	1393:1406	arg1	line					1424:1427	preosteoblasts (MC3T3-E1) cell line	1393:1427	preosteoblasts (MC3T3-E1) cell line	1393:1427	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	7	20	theme	hyaluronic	1081:1090	arg1	acid-gelatin					1092:1103	loading hyaluronic acid-gelatin	1073:1103	loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution	1073:1201	The mechanical strength of biphasic calcium phosphate granule increased after loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution.
28944711	1	21	theme	ideal	136:140	arg1	bone					142:145	An ideal bone substitute	133:156	An ideal bone substitute	133:156	An ideal bone substitute should be made of biocompatible materials that mimic the structure, characteristics, and functions of natural bone.
28944711	1	22	theme	natural	260:266	arg1	bone					268:271	natural bone	260:271	natural bone	260:271	An ideal bone substitute should be made of biocompatible materials that mimic the structure, characteristics, and functions of natural bone.
28944711	5	23	theme	hyaluronic	841:850	arg1	acid-gelatin					852:863	hyaluronic acid-gelatin	841:863	hyaluronic acid-gelatin	841:863	This combination of biphasic calcium phosphate and hyaluronic acid-gelatin retained suitable characteristics for bone regeneration.
28944711	9	24	theme	cell	1419:1422	arg1	MC3T3-E1					1409:1416	MC3T3-E1	1409:1416	MC3T3-E1	1409:1416	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	9	24	theme	cell	1419:1422	arg1	line					1424:1427	preosteoblasts (MC3T3-E1) cell line	1393:1427	preosteoblasts (MC3T3-E1) cell line	1393:1427	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	7	25	theme	granule	1049:1055	arg1	strength					1010:1017	The mechanical strength	995:1017	The mechanical strength of biphasic calcium phosphate granule	995:1055	The mechanical strength of biphasic calcium phosphate granule increased after loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution.
28944711	11	26	theme	acid-gelatin	1974:1985	arg1	scaffolds					1987:1995	biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds	1936:1995	biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds	1936:1995	Histological section confirmed the formation of dense collagenous tissue and new bone in biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds at two months.
28944711	0	27	theme	acid-gelatin	88:99	arg1	hydrogel					101:108	hyaluronic acid-gelatin hydrogel	77:108	hyaluronic acid-gelatin hydrogel for bone regeneration	77:130	A hybrid composite system of biphasic calcium phosphate granules loaded with hyaluronic acid-gelatin hydrogel for bone regeneration.
28944711	7	28	theme	calcium	1031:1037	arg1	granule					1049:1055	biphasic calcium phosphate granule	1022:1055	biphasic calcium phosphate granule	1022:1055	The mechanical strength of biphasic calcium phosphate granule increased after loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution.
28944711	10	29	from	samples	1702:1708	arg1	Analysis					1612:1619	Analysis	1612:1619	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples	1612:1708	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	10	29	from	samples	1702:1708	arg1	sections					1674:1681	stained tissue sections	1659:1681	stained tissue sections	1659:1681	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	10	29	from	samples	1702:1708	arg1	data					1650:1653	micro-computed tomography data	1624:1653	micro-computed tomography data	1624:1653	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	3	30	theme	acid-gelatin	447:458	arg1	hydrogel					460:467	hyaluronic acid-gelatin hydrogel	436:467	hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules	436:522	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	3	30	theme	acid-gelatin	447:458	arg1	carrier					529:535	a carrier	527:535	a carrier to improve cell attachment and proliferation through highly interconnected porous structure	527:627	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	5	31	theme	biphasic	810:817	arg1	phosphate					827:835	biphasic calcium phosphate	810:835	biphasic calcium phosphate	810:835	This combination of biphasic calcium phosphate and hyaluronic acid-gelatin retained suitable characteristics for bone regeneration.
28944711	0	32	theme	biphasic	29:36	arg1	granules					56:63	biphasic calcium phosphate granules	29:63	biphasic calcium phosphate granules	29:63	A hybrid composite system of biphasic calcium phosphate granules loaded with hyaluronic acid-gelatin hydrogel for bone regeneration.
28944711	11	33	theme	phosphate/hyaluronic	1953:1972	arg1	scaffolds					1987:1995	biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds	1936:1995	biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds	1936:1995	Histological section confirmed the formation of dense collagenous tissue and new bone in biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds at two months.
28944711	10	34	theme	tomography	1639:1648	arg1	data					1650:1653	micro-computed tomography data	1624:1653	micro-computed tomography data	1624:1653	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	10	35	theme	months	1822:1827	arg1	postimplantation					1829:1844	the scaffold one and two months postimplantation	1797:1844	postimplantation	1829:1844	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	5	36	theme	bone	903:906	arg1	regeneration					908:919	bone regeneration	903:919	bone regeneration	903:919	This combination of biphasic calcium phosphate and hyaluronic acid-gelatin retained suitable characteristics for bone regeneration.
28944711	12	37	theme	bone	2138:2141	arg1	regeneration					2143:2154	bone regeneration	2138:2154	bone regeneration	2138:2154	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	7	38	theme	mechanical	999:1008	arg1	strength					1010:1017	The mechanical strength	995:1017	The mechanical strength of biphasic calcium phosphate granule	995:1055	The mechanical strength of biphasic calcium phosphate granule increased after loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution.
28944711	1	39	dep	bone	142:145	arg1	substitute					147:156	substitute	147:156	substitute	147:156	An ideal bone substitute should be made of biocompatible materials that mimic the structure, characteristics, and functions of natural bone.
28944711	3	40	theme	biphasic	488:495	arg1	granules					515:522	micro-channeled biphasic calcium phosphate granules	472:522	micro-channeled biphasic calcium phosphate granules	472:522	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	0	41	theme	composite	9:17	arg1	system					19:24	A hybrid composite system	0:24	A hybrid composite system of biphasic calcium phosphate granules	0:63	A hybrid composite system of biphasic calcium phosphate granules loaded with hyaluronic acid-gelatin hydrogel for bone regeneration.
28944711	9	42	theme	calcium	1467:1473	arg1	acid-gelatin					1496:1507	biphasic calcium phosphate/hyaluronic acid-gelatin	1458:1507	biphasic calcium phosphate/hyaluronic acid-gelatin	1458:1507	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	3	43	dep	cell	548:551	arg1	attachment					553:562	attachment	553:562	attachment	553:562	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	0	44	theme	granules	56:63	arg1	system					19:24	A hybrid composite system	0:24	A hybrid composite system of biphasic calcium phosphate granules	0:63	A hybrid composite system of biphasic calcium phosphate granules loaded with hyaluronic acid-gelatin hydrogel for bone regeneration.
28944711	10	45	theme	tissue	1667:1672	arg1	sections					1674:1681	stained tissue sections	1659:1681	stained tissue sections	1659:1681	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	11	46	theme	collagenous	1901:1911	arg1	tissue					1913:1918	dense collagenous tissue	1895:1918	dense collagenous tissue	1895:1918	Histological section confirmed the formation of dense collagenous tissue and new bone in biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds at two months.
28944711	8	47	theme	Swelling	1204:1211	arg1	rates					1229:1233	Swelling and degradation rates	1204:1233	Swelling and degradation rates	1204:1233	Swelling and degradation rates decreased in the hybrid scaffold compared to hydrogel due to the presence of granules in hybrid scaffold.
28944711	3	48	theme	present	405:411	arg1	study					413:417	the present study	401:417	the present study	401:417	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	5	49	theme	phosphate	827:835	arg1	combination					795:805	This combination	790:805	This combination of biphasic calcium phosphate and hyaluronic acid-gelatin	790:863	This combination of biphasic calcium phosphate and hyaluronic acid-gelatin retained suitable characteristics for bone regeneration.
28944711	2	50	theme	scaffold	340:347	arg1	system					390:395	ceramic-polymer hybrid system	367:395	ceramic-polymer hybrid system	367:395	Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system.
28944711	2	50	theme	scaffold	340:347	arg1	systems					349:355	different bone scaffold systems	325:355	different bone scaffold systems including ceramic-polymer hybrid system	325:395	Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system.
28944711	0	51	theme	calcium	38:44	arg1	granules					56:63	biphasic calcium phosphate granules	29:63	biphasic calcium phosphate granules	29:63	A hybrid composite system of biphasic calcium phosphate granules loaded with hyaluronic acid-gelatin hydrogel for bone regeneration.
28944711	8	52	theme	degradation	1217:1227	arg1	rates					1229:1233	Swelling and degradation rates	1204:1233	Swelling and degradation rates	1204:1233	Swelling and degradation rates decreased in the hybrid scaffold compared to hydrogel due to the presence of granules in hybrid scaffold.
28944711	12	53	theme	hybrid	2045:2050	arg1	system					2127:2132	a promising system	2115:2132	a promising system for bone regeneration	2115:2154	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	12	53	theme	hybrid	2045:2050	arg1	scaffold					2103:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	10	54	theme	implanted	1692:1700	arg1	samples					1702:1708	the implanted samples	1688:1708	the implanted samples	1688:1708	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	11	55	theme	bone	1928:1931	arg1	formation					1882:1890	the formation	1878:1890	the formation of dense collagenous tissue and new bone	1878:1931	Histological section confirmed the formation of dense collagenous tissue and new bone in biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds at two months.
28944711	2	56	theme	different	325:333	arg1	system					390:395	ceramic-polymer hybrid system	367:395	ceramic-polymer hybrid system	367:395	Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system.
28944711	2	56	theme	different	325:333	arg1	systems					349:355	different bone scaffold systems	325:355	different bone scaffold systems including ceramic-polymer hybrid system	325:395	Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system.
28944711	12	57	theme	phosphate/hyaluronic	2069:2088	arg1	system					2127:2132	a promising system	2115:2132	a promising system for bone regeneration	2115:2154	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	12	57	theme	phosphate/hyaluronic	2069:2088	arg1	scaffold					2103:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	6	58	theme	resulting	926:934	arg1	scaffold					936:943	The resulting scaffold	922:943	The resulting scaffold	922:943	The resulting scaffold had a porosity of 56% with a suitable pore sizes.
28944711	2	59	theme	hybrid	383:388	arg1	system					390:395	ceramic-polymer hybrid system	367:395	ceramic-polymer hybrid system	367:395	Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system.
28944711	0	60	theme	phosphate	46:54	arg1	granules					56:63	biphasic calcium phosphate granules	29:63	biphasic calcium phosphate granules	29:63	A hybrid composite system of biphasic calcium phosphate granules loaded with hyaluronic acid-gelatin hydrogel for bone regeneration.
28944711	8	61	from	granules	1312:1319	arg1	scaffold					1331:1338	hybrid scaffold	1324:1338	hybrid scaffold	1324:1338	Swelling and degradation rates decreased in the hybrid scaffold compared to hydrogel due to the presence of granules in hybrid scaffold.
28944711	8	62	from	presence	1300:1307	arg1	scaffold					1331:1338	hybrid scaffold	1324:1338	hybrid scaffold	1324:1338	Swelling and degradation rates decreased in the hybrid scaffold compared to hydrogel due to the presence of granules in hybrid scaffold.
28944711	9	63	theme	calcium	1584:1590	arg1	granules					1602:1609	biphasic calcium phosphate granules	1575:1609	biphasic calcium phosphate granules	1575:1609	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	6	64	theme	suitable	974:981	arg1	sizes					988:992	a suitable pore sizes	972:992	a suitable pore sizes	972:992	The resulting scaffold had a porosity of 56% with a suitable pore sizes.
28944711	4	65	theme	hybrid	635:640	arg1	system					642:647	This hybrid system	630:647	This hybrid system	630:647	This hybrid system is composed of ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel.
28944711	10	66	theme	good	1746:1749	arg1	osseointegration					1751:1766	good osseointegration	1746:1766	good osseointegration	1746:1766	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	2	67	theme	Many	274:277	arg1	researchers					279:289	Many researchers	274:289	Many researchers	274:289	Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system.
28944711	3	68	theme	porous	612:617	arg1	structure					619:627	highly interconnected porous structure	590:627	highly interconnected porous structure	590:627	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	12	69	theme	biphasic	2052:2059	arg1	system					2127:2132	a promising system	2115:2132	a promising system for bone regeneration	2115:2154	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	12	69	theme	biphasic	2052:2059	arg1	scaffold					2103:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	1	70	theme	biocompatible	176:188	arg1	materials					190:198	biocompatible materials	176:198	biocompatible materials that mimic the structure, characteristics, and functions of natural bone	176:271	An ideal bone substitute should be made of biocompatible materials that mimic the structure, characteristics, and functions of natural bone.
28944711	10	71	theme	bone	1779:1782	arg1	formation					1784:1792	better bone formation	1772:1792	better bone formation	1772:1792	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	7	72	theme	cancellous	1174:1183	arg1	substitution					1190:1201	cancellous bone substitution	1174:1201	cancellous bone substitution	1174:1201	The mechanical strength of biphasic calcium phosphate granule increased after loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution.
28944711	4	73	theme	biphasic	672:679	arg1	phosphate					689:697	ceramic biphasic calcium phosphate	664:697	ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel	664:787	This hybrid system is composed of ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel.
28944711	9	74	theme	cytocompatibility	1350:1366	arg1	studies					1368:1374	In vitro cytocompatibility studies	1341:1374	In vitro cytocompatibility studies	1341:1374	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	4	75	theme	phosphate	689:697	arg1	granules					699:706	ceramic biphasic calcium phosphate granules	664:706	ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel	664:787	This hybrid system is composed of ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel.
28944711	4	76	theme	hyaluronic	756:765	arg1	hydrogel					780:787	hyaluronic acid-gelatin hydrogel	756:787	hyaluronic acid-gelatin hydrogel	756:787	This hybrid system is composed of ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel.
28944711	3	77	theme	phosphate	505:513	arg1	granules					515:522	micro-channeled biphasic calcium phosphate granules	472:522	micro-channeled biphasic calcium phosphate granules	472:522	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	5	78	theme	calcium	819:825	arg1	phosphate					827:835	biphasic calcium phosphate	810:835	biphasic calcium phosphate	810:835	This combination of biphasic calcium phosphate and hyaluronic acid-gelatin retained suitable characteristics for bone regeneration.
28944711	11	79	theme	biphasic	1936:1943	arg1	scaffolds					1987:1995	biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds	1936:1995	biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds	1936:1995	Histological section confirmed the formation of dense collagenous tissue and new bone in biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds at two months.
28944711	0	80	theme	hyaluronic	77:86	arg1	hydrogel					101:108	hyaluronic acid-gelatin hydrogel	77:108	hyaluronic acid-gelatin hydrogel for bone regeneration	77:130	A hybrid composite system of biphasic calcium phosphate granules loaded with hyaluronic acid-gelatin hydrogel for bone regeneration.
28944711	7	81	theme	loading	1073:1079	arg1	acid-gelatin					1092:1103	loading hyaluronic acid-gelatin	1073:1103	loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution	1073:1201	The mechanical strength of biphasic calcium phosphate granule increased after loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution.
28944711	6	82	contain	had	945:947	arg1	scaffold					936:943	The resulting scaffold	922:943	The resulting scaffold	922:943	The resulting scaffold had a porosity of 56% with a suitable pore sizes.
28944711	6	82	contain	had	945:947	arg2	porosity					951:958	a porosity	949:958	a porosity of 56% with a suitable pore sizes	949:992	The resulting scaffold had a porosity of 56% with a suitable pore sizes.
28944711	0	83	theme	bone	114:117	arg1	regeneration					119:130	bone regeneration	114:130	bone regeneration	114:130	A hybrid composite system of biphasic calcium phosphate granules loaded with hyaluronic acid-gelatin hydrogel for bone regeneration.
28944711	7	84	theme	phosphate	1039:1047	arg1	granule					1049:1055	biphasic calcium phosphate granule	1022:1055	biphasic calcium phosphate granule	1022:1055	The mechanical strength of biphasic calcium phosphate granule increased after loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution.
28944711	9	85	theme	phosphate	1592:1600	arg1	granules					1602:1609	biphasic calcium phosphate granules	1575:1609	biphasic calcium phosphate granules	1575:1609	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	6	86	with	porosity	951:958	arg1	sizes					988:992	a suitable pore sizes	972:992	a suitable pore sizes	972:992	The resulting scaffold had a porosity of 56% with a suitable pore sizes.
28944711	10	87	theme	data	1650:1653	arg1	Analysis					1612:1619	Analysis	1612:1619	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples	1612:1708	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	0	88	theme	hybrid	2:7	arg1	system					19:24	A hybrid composite system	0:24	A hybrid composite system of biphasic calcium phosphate granules	0:63	A hybrid composite system of biphasic calcium phosphate granules loaded with hyaluronic acid-gelatin hydrogel for bone regeneration.
28944711	7	89	theme	biphasic	1022:1029	arg1	granule					1049:1055	biphasic calcium phosphate granule	1022:1055	biphasic calcium phosphate granule	1022:1055	The mechanical strength of biphasic calcium phosphate granule increased after loading hyaluronic acid-gelatin from 4.26 ± 0.43 to 6.57 ± 0.25 MPa, which is highly recommended for cancellous bone substitution.
28944711	10	90	contain	had	1742:1744	arg2	osseointegration					1751:1766	good osseointegration	1746:1766	good osseointegration	1746:1766	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	10	90	contain	had	1742:1744	arg1	scaffold					1733:1740	the hybrid scaffold	1722:1740	the hybrid scaffold	1722:1740	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	10	90	contain	had	1742:1744	arg2	formation					1784:1792	better bone formation	1772:1792	better bone formation	1772:1792	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	10	91	theme	micro-computed	1624:1637	arg1	tomography					1639:1648	micro-computed tomography	1624:1648	micro-computed tomography data	1624:1653	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	3	92	theme	hyaluronic	436:445	arg1	hydrogel					460:467	hyaluronic acid-gelatin hydrogel	436:467	hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules	436:522	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	3	92	theme	hyaluronic	436:445	arg1	carrier					529:535	a carrier	527:535	a carrier to improve cell attachment and proliferation through highly interconnected porous structure	527:627	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	9	93	theme	biphasic	1458:1465	arg1	acid-gelatin					1496:1507	biphasic calcium phosphate/hyaluronic acid-gelatin	1458:1507	biphasic calcium phosphate/hyaluronic acid-gelatin	1458:1507	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	10	94	theme	sections	1674:1681	arg1	Analysis					1612:1619	Analysis	1612:1619	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples	1612:1708	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	11	95	theme	dense	1895:1899	arg1	tissue					1913:1918	dense collagenous tissue	1895:1918	dense collagenous tissue	1895:1918	Histological section confirmed the formation of dense collagenous tissue and new bone in biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds at two months.
28944711	3	96	theme	calcium	497:503	arg1	granules					515:522	micro-channeled biphasic calcium phosphate granules	472:522	micro-channeled biphasic calcium phosphate granules	472:522	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	5	97	theme	acid-gelatin	852:863	arg1	combination					795:805	This combination	790:805	This combination of biphasic calcium phosphate and hyaluronic acid-gelatin	790:863	This combination of biphasic calcium phosphate and hyaluronic acid-gelatin retained suitable characteristics for bone regeneration.
28944711	9	98	theme	phosphate/hyaluronic	1475:1494	arg1	acid-gelatin					1496:1507	biphasic calcium phosphate/hyaluronic acid-gelatin	1458:1507	biphasic calcium phosphate/hyaluronic acid-gelatin	1458:1507	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	12	99	theme	promising	2117:2125	arg1	system					2127:2132	a promising system	2115:2132	a promising system for bone regeneration	2115:2154	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	12	99	theme	promising	2117:2125	arg1	scaffold					2103:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	10	100	theme	stained	1659:1665	arg1	sections					1674:1681	stained tissue sections	1659:1681	stained tissue sections	1659:1681	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	4	101	with	1 mm	718:721	arg1	holes					746:750	seven holes	740:750	seven holes	740:750	This hybrid system is composed of ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel.
28944711	4	101	with	1 mm	718:721	arg1	hydrogel					780:787	hyaluronic acid-gelatin hydrogel	756:787	hyaluronic acid-gelatin hydrogel	756:787	This hybrid system is composed of ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel.
28944711	3	102	theme	micro-channeled	472:486	arg1	granules					515:522	micro-channeled biphasic calcium phosphate granules	472:522	micro-channeled biphasic calcium phosphate granules	472:522	In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure.
28944711	5	103	theme	suitable	874:881	arg1	characteristics					883:897	suitable characteristics	874:897	suitable characteristics for bone regeneration	874:919	This combination of biphasic calcium phosphate and hyaluronic acid-gelatin retained suitable characteristics for bone regeneration.
28944711	12	104	theme	calcium	2061:2067	arg1	system					2127:2132	a promising system	2115:2132	a promising system for bone regeneration	2115:2154	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	12	104	theme	calcium	2061:2067	arg1	scaffold					2103:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	11	105	theme	new	1924:1926	arg1	bone					1928:1931	new bone	1924:1931	new bone	1924:1931	Histological section confirmed the formation of dense collagenous tissue and new bone in biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds at two months.
28944711	2	106	theme	bone	335:338	arg1	system					390:395	ceramic-polymer hybrid system	367:395	ceramic-polymer hybrid system	367:395	Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system.
28944711	2	106	theme	bone	335:338	arg1	systems					349:355	different bone scaffold systems	325:355	different bone scaffold systems including ceramic-polymer hybrid system	325:395	Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system.
28944711	9	107	theme	cell	1533:1536	arg1	growth					1538:1543	cell growth	1533:1543	cell growth	1533:1543	In vitro cytocompatibility studies were observed by preosteoblasts (MC3T3-E1) cell line and the result revealed that biphasic calcium phosphate/hyaluronic acid-gelatin significantly increased cell growth and proliferation compared to biphasic calcium phosphate granules.
28944711	12	108	theme	acid-gelatin	2090:2101	arg1	system					2127:2132	a promising system	2115:2132	a promising system for bone regeneration	2115:2154	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	12	108	theme	acid-gelatin	2090:2101	arg1	scaffold					2103:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	12	109	theme	such	2040:2043	arg1	system					2127:2132	a promising system	2115:2132	a promising system for bone regeneration	2115:2154	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	12	109	theme	such	2040:2043	arg1	scaffold					2103:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold	2040:2110	Our study demonstrated that such hybrid biphasic calcium phosphate/hyaluronic acid-gelatin scaffold is a promising system for bone regeneration.
28944711	11	110	theme	tissue	1913:1918	arg1	formation					1882:1890	the formation	1878:1890	the formation of dense collagenous tissue and new bone	1878:1931	Histological section confirmed the formation of dense collagenous tissue and new bone in biphasic calcium phosphate/hyaluronic acid-gelatin scaffolds at two months.
28944711	1	111	dep	structure	215:223	arg1	the					211:213	the	211:213	the	211:213	An ideal bone substitute should be made of biocompatible materials that mimic the structure, characteristics, and functions of natural bone.
28944711	8	112	theme	hybrid	1252:1257	arg1	scaffold					1259:1266	the hybrid scaffold	1248:1266	the hybrid scaffold	1248:1266	Swelling and degradation rates decreased in the hybrid scaffold compared to hydrogel due to the presence of granules in hybrid scaffold.
28944711	4	113	theme	ceramic	664:670	arg1	phosphate					689:697	ceramic biphasic calcium phosphate	664:697	ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel	664:787	This hybrid system is composed of ceramic biphasic calcium phosphate granules measuring 1 mm in diameter with seven holes and hyaluronic acid-gelatin hydrogel.
28944711	10	114	theme	hybrid	1726:1731	arg1	scaffold					1733:1740	the hybrid scaffold	1722:1740	the hybrid scaffold	1722:1740	Analysis of micro-computed tomography data and stained tissue sections from the implanted samples showed that the hybrid scaffold had good osseointegration and better bone formation in the scaffold one and two months postimplantation.
28944711	2	115	theme	ceramic-polymer	367:381	arg1	system					390:395	ceramic-polymer hybrid system	367:395	ceramic-polymer hybrid system	367:395	Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system.
28933537	0	0	theme	Nanoparticle	76:87	arg1	Transducers					89:99	Plasmonic Nanoparticle Transducers	66:99	Plasmonic Nanoparticle Transducers	66:99	Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers.
28933537	0	1	from	Sensing	18:24	arg1	Level					55:59	the Single Virus Particle Level	29:59	the Single Virus Particle Level with Plasmonic Nanoparticle Transducers	29:99	Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers.
28933537	4	2	from	effect	793:798	arg1	fluidity					832:839	the membrane fluidity	819:839	the membrane fluidity	819:839	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	4	3	theme	polarization	698:709	arg1	PFTM					744:747	PFTM	744:747	PFTM	744:747	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	4	3	theme	polarization	698:709	arg1	microscopy					732:741	plasmonic polarization fluctuation tracking microscopy	688:741	plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level	688:917	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	0	4	theme	Plasmonic	66:74	arg1	Transducers					89:99	Plasmonic Nanoparticle Transducers	66:99	Plasmonic Nanoparticle Transducers	66:99	Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers.
28933537	6	5	theme	MβCD	1285:1288	arg1	concentration					1290:1302	MβCD concentration	1285:1302	MβCD concentration	1285:1302	The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.
28933537	6	6	theme	extraction	1099:1108	arg1	rate					1086:1089	The rate	1082:1089	The rate of chol extraction for individual VLPs	1082:1128	The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.
28933537	2	7	theme	biophysical	344:354	arg1	tools					356:360	biophysical tools	344:360	biophysical tools capable of sensing the membrane fluidity on the single-virus level	344:427	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28933537	5	8	theme	time	1076:1079	arg1	function					1064:1071	a function	1062:1071	a function of time	1062:1079	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	5	8	theme	time	1076:1079	arg1	decrease					1021:1028	a gradual decrease	1011:1028	a gradual decrease in polarization fluctuations	1011:1057	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	6	9	theme	chol	1094:1097	arg1	extraction					1099:1108	chol extraction	1094:1108	chol extraction for individual VLPs	1094:1128	The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.
28933537	4	10	theme	effect	793:798	arg1	dependence					849:858	its dependence	845:858	its dependence on temperature	845:873	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	4	10	theme	effect	793:798	arg1	investigation					772:784	the investigation	768:784	the investigation of the effect of chol content on the membrane fluidity	768:839	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	6	11	theme	individual	1219:1228	arg1	VLPs					1230:1233	the individual VLPs	1215:1233	the individual VLPs	1215:1233	The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.
28933537	2	12	theme	virus	285:289	arg1	particles					291:299	individual virus particles	274:299	individual virus particles	274:299	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28933537	5	13	theme	different	949:957	arg1	concentrations					988:1001	different methyl-β-cyclodextrin (MβCD) concentrations	949:1001	different methyl-β-cyclodextrin (MβCD) concentrations	949:1001	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	4	14	from	investigation	772:784	arg1	temperature					863:873	temperature	863:873	temperature	863:873	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	4	14	from	investigation	772:784	arg1	fluidity					832:839	the membrane fluidity	819:839	the membrane fluidity	819:839	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	2	15	theme	individual	274:283	arg1	particles					291:299	individual virus particles	274:299	individual virus particles	274:299	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28933537	5	16	theme	Chol	920:923	arg1	studies					936:942	Chol extraction studies	920:942	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations	920:1001	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	4	17	theme	plasmonic	688:696	arg1	PFTM					744:747	PFTM	744:747	PFTM	744:747	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	4	17	theme	plasmonic	688:696	arg1	microscopy					732:741	plasmonic polarization fluctuation tracking microscopy	688:741	plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level	688:917	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	4	18	from	dependence	849:858	arg1	temperature					863:873	temperature	863:873	temperature	863:873	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	4	18	from	dependence	849:858	arg1	fluidity					832:839	the membrane fluidity	819:839	the membrane fluidity	819:839	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	2	19	theme	single-virus	410:421	arg1	level					423:427	the single-virus level	406:427	the single-virus level	406:427	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28933537	5	20	theme	MβCD	982:985	arg1	concentrations					988:1001	different methyl-β-cyclodextrin (MβCD) concentrations	949:1001	different methyl-β-cyclodextrin (MβCD) concentrations	949:1001	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	1	21	dep	nanomaterials	122:134	arg1	content					219:225	the cholesterol (chol) content	196:225	the cholesterol (chol) content	196:225	Viral membranes are nanomaterials whose fluidity depends on their composition, in particular, the cholesterol (chol) content.
28933537	2	22	theme	membrane	385:392	arg1	fluidity					394:401	the membrane fluidity	381:401	the membrane fluidity on the single-virus level	381:427	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28933537	3	23	theme	light	519:523	arg1	polarization					503:514	the polarization	499:514	the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs)	499:606	In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs.
28933537	5	24	theme	extraction	925:934	arg1	studies					936:942	Chol extraction studies	920:942	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations	920:1001	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	6	25	from	differences	1171:1181	arg1	composition					1199:1209	the membrane composition	1186:1209	the membrane composition for the individual VLPs	1186:1233	The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.
28933537	3	26	dep	virus-like-particles	580:599	arg1	off					535:537	off	535:537	off	535:537	In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs.
28933537	0	27	theme	Membrane	0:7	arg1	Fluidity					9:16	Membrane Fluidity	0:16	Membrane Fluidity	0:16	Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers.
28933537	3	28	from	fluctuations	483:494	arg1	polarization					503:514	the polarization	499:514	the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs)	499:606	In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs.
28933537	6	29	theme	membrane	1190:1197	arg1	composition					1199:1209	the membrane composition	1186:1209	the membrane composition for the individual VLPs	1186:1233	The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.
28933537	1	30	from	nanomaterials	122:134	arg1	particular					184:193	particular	184:193	particular	184:193	Viral membranes are nanomaterials whose fluidity depends on their composition, in particular, the cholesterol (chol) content.
28933537	1	31	theme	Viral	102:106	arg1	nanomaterials					122:134	nanomaterials	122:134	nanomaterials whose fluidity depends on their composition	122:178	Viral membranes are nanomaterials whose fluidity depends on their composition, in particular, the cholesterol (chol) content.
28933537	1	31	theme	Viral	102:106	arg1	membranes					108:116	Viral membranes	102:116	Viral membranes	102:116	Viral membranes are nanomaterials whose fluidity depends on their composition, in particular, the cholesterol (chol) content.
28933537	2	32	from	differences	231:241	arg1	composition					259:269	the membrane composition	246:269	the membrane composition of individual virus particles	246:299	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28933537	5	33	theme	methyl-β-cyclodextrin	959:979	arg1	concentrations					988:1001	different methyl-β-cyclodextrin (MβCD) concentrations	949:1001	different methyl-β-cyclodextrin (MβCD) concentrations	949:1001	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	5	34	with	studies	936:942	arg1	concentrations					988:1001	different methyl-β-cyclodextrin (MβCD) concentrations	949:1001	different methyl-β-cyclodextrin (MβCD) concentrations	949:1001	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	4	35	theme	single-VLP	902:911	arg1	level					913:917	the single-VLP level	898:917	the single-VLP level	898:917	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	3	36	theme	membrane	637:644	arg1	fluidity					646:653	the membrane fluidity	633:653	the membrane fluidity of individual VLPs	633:672	In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs.
28933537	2	37	theme	particles	291:299	arg1	composition					259:269	the membrane composition	246:269	the membrane composition of individual virus particles	246:299	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28933537	5	38	from	decrease	1021:1028	arg1	fluctuations					1046:1057	polarization fluctuations	1033:1057	polarization fluctuations	1033:1057	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	1	39	theme	cholesterol	200:210	arg1	content					219:225	the cholesterol (chol) content	196:225	the cholesterol (chol) content	196:225	Viral membranes are nanomaterials whose fluidity depends on their composition, in particular, the cholesterol (chol) content.
28933537	0	40	theme	Single	33:38	arg1	Level					55:59	the Single Virus Particle Level	29:59	the Single Virus Particle Level with Plasmonic Nanoparticle Transducers	29:99	Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers.
28933537	4	41	from	fluidity	832:839	arg1	dependence					849:858	its dependence	845:858	its dependence on temperature	845:873	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	4	41	from	fluidity	832:839	arg1	investigation					772:784	the investigation	768:784	the investigation of the effect of chol content on the membrane fluidity	768:839	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	1	42	theme	chol	213:216	arg1	content					219:225	the cholesterol (chol) content	196:225	the cholesterol (chol) content	196:225	Viral membranes are nanomaterials whose fluidity depends on their composition, in particular, the cholesterol (chol) content.
28933537	0	43	theme	Particle	46:53	arg1	Level					55:59	the Single Virus Particle Level	29:59	the Single Virus Particle Level with Plasmonic Nanoparticle Transducers	29:99	Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers.
28933537	4	44	theme	membrane	823:830	arg1	fluidity					832:839	the membrane fluidity	819:839	the membrane fluidity	819:839	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	5	45	theme	polarization	1033:1044	arg1	fluctuations					1046:1057	polarization fluctuations	1033:1057	polarization fluctuations	1033:1057	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	4	46	theme	tracking	723:730	arg1	PFTM					744:747	PFTM	744:747	PFTM	744:747	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	4	46	theme	tracking	723:730	arg1	microscopy					732:741	plasmonic polarization fluctuation tracking microscopy	688:741	plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level	688:917	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	0	47	theme	Virus	40:44	arg1	Level					55:59	the Single Virus Particle Level	29:59	the Single Virus Particle Level with Plasmonic Nanoparticle Transducers	29:99	Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers.
28933537	4	48	theme	first	884:888	arg1	time					890:893	the first time	880:893	the first time on the single-VLP level	880:917	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	3	49	theme	VLPs	669:672	arg1	fluidity					646:653	the membrane fluidity	633:653	the membrane fluidity of individual VLPs	633:672	In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs.
28933537	3	50	theme	-labeled	571:578	arg1	virus-like-particles					580:599	gold or silver nanoparticle (NP)-labeled virus-like-particles	539:599	gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs)	539:606	In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs.
28933537	3	50	theme	-labeled	571:578	arg1	VLPs					602:605	VLPs	602:605	VLPs	602:605	In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs.
28933537	2	51	from	fluidity	394:401	arg1	level					423:427	the single-virus level	406:427	the single-virus level	406:427	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28933537	4	52	theme	fluctuation	711:721	arg1	PFTM					744:747	PFTM	744:747	PFTM	744:747	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	4	52	theme	fluctuation	711:721	arg1	microscopy					732:741	plasmonic polarization fluctuation tracking microscopy	688:741	plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level	688:917	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	5	53	theme	gradual	1013:1019	arg1	function					1064:1071	a function	1062:1071	a function of time	1062:1079	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	5	53	theme	gradual	1013:1019	arg1	decrease					1021:1028	a gradual decrease	1011:1028	a gradual decrease in polarization fluctuations	1011:1057	Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as a function of time.
28933537	2	54	theme	membrane	250:257	arg1	composition					259:269	the membrane composition	246:269	the membrane composition of individual virus particles	246:299	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28933537	2	55	theme	intracellular	323:335	arg1	fates					337:341	different intracellular fates	313:341	different intracellular fates	313:341	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28933537	6	56	theme	individual	1114:1123	arg1	VLPs					1125:1128	individual VLPs	1114:1128	individual VLPs	1114:1128	The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.
28933537	6	57	theme	due	1164:1166	arg1	spread					1145:1150	a broad spread	1137:1150	a broad spread	1137:1150	The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.
28933537	4	58	from	time	890:893	arg1	level					913:917	the single-VLP level	898:917	the single-VLP level	898:917	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	3	59	theme	gold	539:542	arg1	virus-like-particles					580:599	gold or silver nanoparticle (NP)-labeled virus-like-particles	539:599	gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs)	539:606	In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs.
28933537	3	59	theme	gold	539:542	arg1	VLPs					602:605	VLPs	602:605	VLPs	602:605	In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs.
28933537	2	60	theme	different	313:321	arg1	fates					337:341	different intracellular fates	313:341	different intracellular fates	313:341	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28933537	0	61	with	Level	55:59	arg1	Transducers					89:99	Plasmonic Nanoparticle Transducers	66:99	Plasmonic Nanoparticle Transducers	66:99	Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers.
28933537	3	62	theme	individual	658:667	arg1	VLPs					669:672	individual VLPs	658:672	individual VLPs	658:672	In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs.
28933537	4	63	theme	content	808:814	arg1	effect					793:798	the effect	789:798	the effect of chol content on the membrane fluidity	789:839	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	6	64	theme	broad	1139:1143	arg1	spread					1145:1150	a broad spread	1137:1150	a broad spread	1137:1150	The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.
28933537	4	65	theme	chol	803:806	arg1	content					808:814	chol content	803:814	chol content	803:814	We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level.
28933537	2	66	theme	capable	362:368	arg1	tools					356:360	biophysical tools	344:360	biophysical tools capable of sensing the membrane fluidity on the single-virus level	344:427	As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required.
28287501	0	0	theme	DNA	92:94	arg1	Delivery					96:103	Plasmid DNA Delivery	84:103	Plasmid DNA Delivery	84:103	Evaluation of Maltose-Based Cationic Liposomes with Different Hydrophobic Tails for Plasmid DNA Delivery.
28287501	3	1	theme	scattering	680:689	arg1	results					587:593	The results	583:593	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS)	583:695	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	1	2	theme	different	153:161	arg1	Malt-DiC16MA					227:238	Malt-DiC16MA	227:238	Malt-DiC16MA (IX c)	227:245	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	2	theme	different	153:161	arg1	chains					175:180	different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c)	153:245	different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c)	153:245	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	2	theme	different	153:161	arg1	Malt-DiC12MA					182:193	Malt-DiC12MA	182:193	Malt-DiC12MA (IX a)	182:200	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	2	theme	different	153:161	arg1	Malt-DiC14MA					203:214	Malt-DiC14MA	203:214	Malt-DiC14MA (IX b)	203:221	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	6	3	theme	thiazolyl	1395:1403	arg1	assay					1436:1440	thiazolyl blue tetrazolium bromide (MTT) assay	1395:1440	thiazolyl blue tetrazolium bromide (MTT) assay	1395:1440	Additionally, the result of cell viability showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay.
28287501	0	4	theme	Plasmid	84:90	arg1	Delivery					96:103	Plasmid DNA Delivery	84:103	Plasmid DNA Delivery	84:103	Evaluation of Maltose-Based Cationic Liposomes with Different Hydrophobic Tails for Plasmid DNA Delivery.
28287501	4	5	dep	efficiency	910:919	arg1	The					893:895	The	893:895	The	893:895	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	1	6	theme	Staudinger	416:425	arg1	reaction					427:434	Staudinger reaction	416:434	Staudinger reaction	416:434	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	7	theme	hydrophobic	163:173	arg1	Malt-DiC16MA					227:238	Malt-DiC16MA	227:238	Malt-DiC16MA (IX c)	227:245	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	7	theme	hydrophobic	163:173	arg1	chains					175:180	different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c)	153:245	different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c)	153:245	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	7	theme	hydrophobic	163:173	arg1	Malt-DiC12MA					182:193	Malt-DiC12MA	182:193	Malt-DiC12MA (IX a)	182:200	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	7	theme	hydrophobic	163:173	arg1	Malt-DiC14MA					203:214	Malt-DiC14MA	203:214	Malt-DiC14MA (IX b)	203:221	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	3	8	theme	proper	819:824	arg1	size					826:829	proper size	819:829	proper size (88 nm-146 nm, PDI < 0.4)	819:855	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	3	8	theme	proper	819:824	arg1	nm					842:843	88 nm-146 nm	832:843	88 nm-146 nm	832:843	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	6	9	theme	low	1350:1352	arg1	biotoxicity					1354:1364	low biotoxicity	1350:1364	low biotoxicity	1350:1364	Additionally, the result of cell viability showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay.
28287501	3	10	dep	DNA	767:769	arg1	ratio					776:780	N/P ratio	772:780	N/P ratio less than 2	772:792	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	3	11	theme	dynamic	666:672	arg1	DLS					692:694	DLS	692:694	DLS	692:694	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	3	11	theme	dynamic	666:672	arg1	scattering					680:689	dynamic light scattering	666:689	dynamic light scattering (DLS)	666:695	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	1	12	with	glycolipids	136:146	arg1	Malt-DiC16MA					227:238	Malt-DiC16MA	227:238	Malt-DiC16MA (IX c)	227:245	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	12	with	glycolipids	136:146	arg1	chains					175:180	different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c)	153:245	different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c)	153:245	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	12	with	glycolipids	136:146	arg1	Malt-DiC12MA					182:193	Malt-DiC12MA	182:193	Malt-DiC12MA (IX a)	182:200	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	12	with	glycolipids	136:146	arg1	Malt-DiC14MA					203:214	Malt-DiC14MA	203:214	Malt-DiC14MA (IX b)	203:221	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	5	13	theme	8:1	1273:1275	arg1	ratios					1263:1268	N/P ratios	1259:1268	N/P ratios of 8:1	1259:1275	Importantly, it indicated that Malt-DiC14MA exhibited high gene transfer efficiency and better uptake capability at N/P ratios of 8:1.
28287501	4	14	theme	Green	1086:1090	arg1	Protein					1104:1110	Enhanced Green Fluorescent Protein	1077:1110	Enhanced Green Fluorescent Protein plasmid	1077:1118	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	1	15	theme	IX	241:242	arg1	Malt-DiC16MA					227:238	Malt-DiC16MA	227:238	Malt-DiC16MA (IX c)	227:245	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	15	theme	IX	241:242	arg1	c					244:244	IX c	241:244	IX c	241:244	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	16	theme	selective	322:330	arg1	1-O-deacetylation					332:348	selective 1-O-deacetylation	322:348	selective 1-O-deacetylation	322:348	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	17	theme	tertiary	437:444	arg1	amination					446:454	tertiary amination	437:454	tertiary amination	437:454	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	5	18	theme	transfer	1207:1214	arg1	efficiency					1216:1225	high gene transfer efficiency	1197:1225	high gene transfer efficiency	1197:1225	Importantly, it indicated that Malt-DiC14MA exhibited high gene transfer efficiency and better uptake capability at N/P ratios of 8:1.
28287501	3	19	theme	less	782:785	arg1	ratio					776:780	N/P ratio	772:780	N/P ratio less than 2	772:792	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	3	20	with	nanoparticles	800:812	arg1	size					826:829	proper size	819:829	proper size (88 nm-146 nm, PDI < 0.4)	819:855	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	3	20	with	nanoparticles	800:812	arg1	nm					842:843	88 nm-146 nm	832:843	88 nm-146 nm	832:843	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	3	21	theme	N/P	772:774	arg1	ratio					776:780	N/P ratio	772:780	N/P ratio less than 2	772:792	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	6	22	theme	good	1370:1373	arg1	biocompatibility					1375:1390	good biocompatibility	1370:1390	good biocompatibility	1370:1390	Additionally, the result of cell viability showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay.
28287501	2	23	theme	Target	476:481	arg1	compounds					483:491	Target compounds	476:491	Target compounds	476:491	Target compounds and some intermediates were characterized by ¹H-NMR, 13C-NMR, ¹H-¹H COSY and ¹H-13C HSQC.
28287501	4	24	theme	Protein	1104:1110	arg1	pEGFP-C1					1067:1074	pEGFP-C1	1067:1074	Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images	1055:1126	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	4	24	theme	Protein	1104:1110	arg1	plasmid					1112:1118	Enhanced Green Fluorescent Protein plasmid	1077:1118	Enhanced Green Fluorescent Protein plasmid	1077:1118	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	4	25	theme	cellular	925:932	arg1	uptake					934:939	cellular uptake	925:939	cellular uptake	925:939	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	4	26	from	glycolipids	944:954	arg1	cell					966:969	HEK293 cell	959:969	HEK293 cell	959:969	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	4	27	theme	fluorescent	1013:1023	arg1	protein					1025:1031	green fluorescent protein	1007:1031	the enhanced green fluorescent protein (EGFP) expression	994:1049	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	4	27	theme	fluorescent	1013:1023	arg1	EGFP					1034:1037	EGFP	1034:1037	EGFP	1034:1037	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	1	28	theme	IX	196:197	arg1	Malt-DiC12MA					182:193	Malt-DiC12MA	182:193	Malt-DiC12MA (IX a)	182:200	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	28	theme	IX	196:197	arg1	a					199:199	IX a	196:199	IX a	196:199	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	0	29	theme	Cationic	28:35	arg1	Liposomes					37:45	Maltose-Based Cationic Liposomes	14:45	Maltose-Based Cationic Liposomes	14:45	Evaluation of Maltose-Based Cationic Liposomes with Different Hydrophobic Tails for Plasmid DNA Delivery.
28287501	6	30	dep	showed	1321:1326	arg1	exhibited					1340:1348	exhibited	1340:1348	showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay	1321:1440	Additionally, the result of cell viability showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay.
28287501	4	31	theme	green	1007:1011	arg1	protein					1025:1031	green fluorescent protein	1007:1031	the enhanced green fluorescent protein (EGFP) expression	994:1049	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	4	31	theme	green	1007:1011	arg1	EGFP					1034:1037	EGFP	1034:1037	EGFP	1034:1037	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	0	32	theme	Maltose-Based	14:26	arg1	Liposomes					37:45	Maltose-Based Cationic Liposomes	14:45	Maltose-Based Cationic Liposomes	14:45	Evaluation of Maltose-Based Cationic Liposomes with Different Hydrophobic Tails for Plasmid DNA Delivery.
28287501	4	33	from	uptake	934:939	arg1	cell					966:969	HEK293 cell	959:969	HEK293 cell	959:969	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	3	34	theme	force	632:636	arg1	microscopy					638:647	atomic force microscopy	625:647	atomic force microscopy images (AFM)	625:660	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	5	35	theme	uptake	1238:1243	arg1	capability					1245:1254	better uptake capability	1231:1254	better uptake capability	1231:1254	Importantly, it indicated that Malt-DiC14MA exhibited high gene transfer efficiency and better uptake capability at N/P ratios of 8:1.
28287501	6	36	theme	bromide	1422:1428	arg1	assay					1436:1440	thiazolyl blue tetrazolium bromide (MTT) assay	1395:1440	thiazolyl blue tetrazolium bromide (MTT) assay	1395:1440	Additionally, the result of cell viability showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay.
28287501	4	37	theme	transfection	897:908	arg1	efficiency					910:919	transfection efficiency	897:919	transfection efficiency	897:919	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	5	38	theme	N/P	1259:1261	arg1	ratios					1263:1268	N/P ratios	1259:1268	N/P ratios of 8:1	1259:1275	Importantly, it indicated that Malt-DiC14MA exhibited high gene transfer efficiency and better uptake capability at N/P ratios of 8:1.
28287501	3	39	theme	microscopy	638:647	arg1	AFM					657:659	AFM	657:659	AFM	657:659	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	3	39	theme	microscopy	638:647	arg1	images					649:654	atomic force microscopy images	625:654	atomic force microscopy images (AFM)	625:660	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	4	40	theme	protein	1025:1031	arg1	expression					1040:1049	the enhanced green fluorescent protein (EGFP) expression	994:1049	the enhanced green fluorescent protein (EGFP) expression	994:1049	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	0	41	theme	Liposomes	37:45	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Maltose-Based Cationic Liposomes with Different Hydrophobic Tails for Plasmid DNA Delivery.	0:104	Evaluation of Maltose-Based Cationic Liposomes with Different Hydrophobic Tails for Plasmid DNA Delivery.
28287501	4	42	theme	glycolipids	944:954	arg1	efficiency					910:919	transfection efficiency	897:919	transfection efficiency	897:919	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	4	42	theme	glycolipids	944:954	arg1	uptake					934:939	cellular uptake	925:939	cellular uptake	925:939	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	4	43	theme	Fluorescent	1092:1102	arg1	Protein					1104:1110	Enhanced Green Fluorescent Protein	1077:1110	Enhanced Green Fluorescent Protein plasmid	1077:1118	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	0	44	theme	Hydrophobic	62:72	arg1	Tails					74:78	Different Hydrophobic Tails	52:78	Different Hydrophobic Tails for Plasmid DNA Delivery	52:103	Evaluation of Maltose-Based Cationic Liposomes with Different Hydrophobic Tails for Plasmid DNA Delivery.
28287501	4	45	from	efficiency	910:919	arg1	cell					966:969	HEK293 cell	959:969	HEK293 cell	959:969	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	2	46	theme	¹H-13C	570:575	arg1	HSQC					577:580	¹H-13C HSQC	570:580	¹H-13C HSQC	570:580	Target compounds and some intermediates were characterized by ¹H-NMR, 13C-NMR, ¹H-¹H COSY and ¹H-13C HSQC.
28287501	3	47	theme	atomic	625:630	arg1	microscopy					638:647	atomic force microscopy	625:647	atomic force microscopy images (AFM)	625:660	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	1	48	dep	chains	175:180	arg1	b					220:220	IX b	217:220	IX b	217:220	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	48	dep	chains	175:180	arg1	c					244:244	IX c	241:244	IX c	241:244	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	48	dep	chains	175:180	arg1	a					199:199	IX a	196:199	IX a	196:199	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	48	dep	chains	175:180	arg1	Malt-DiC14MA					203:214	Malt-DiC14MA	203:214	Malt-DiC14MA (IX b)	203:221	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	48	dep	chains	175:180	arg1	Malt-DiC16MA					227:238	Malt-DiC16MA	227:238	Malt-DiC16MA (IX c)	227:245	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	48	dep	chains	175:180	arg1	chains					175:180	different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c)	153:245	different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c)	153:245	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	48	dep	chains	175:180	arg1	Malt-DiC12MA					182:193	Malt-DiC12MA	182:193	Malt-DiC12MA (IX a)	182:200	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	3	49	dep	nm	842:843	arg1	PDI					846:848	PDI < 0.4	846:854	PDI < 0.4	846:854	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	0	50	theme	Different	52:60	arg1	Tails					74:78	Different Hydrophobic Tails	52:78	Different Hydrophobic Tails for Plasmid DNA Delivery	52:103	Evaluation of Maltose-Based Cationic Liposomes with Different Hydrophobic Tails for Plasmid DNA Delivery.
28287501	3	51	theme	zeta	861:864	arg1	mV					888:889	+15 mV-+26 mV	877:889	+15 mV-+26 mV	877:889	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	3	51	theme	zeta	861:864	arg1	potential					866:874	zeta potential	861:874	zeta potential (+15 mV-+26 mV)	861:890	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	5	52	theme	better	1231:1236	arg1	capability					1245:1254	better uptake capability	1231:1254	better uptake capability	1231:1254	Importantly, it indicated that Malt-DiC14MA exhibited high gene transfer efficiency and better uptake capability at N/P ratios of 8:1.
28287501	5	53	theme	high	1197:1200	arg1	efficiency					1216:1225	high gene transfer efficiency	1197:1225	high gene transfer efficiency	1197:1225	Importantly, it indicated that Malt-DiC14MA exhibited high gene transfer efficiency and better uptake capability at N/P ratios of 8:1.
28287501	3	54	theme	electrophoresis	602:616	arg1	assay					618:622	gel electrophoresis assay	598:622	gel electrophoresis assay	598:622	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	0	55	with	Evaluation	0:9	arg1	Tails					74:78	Different Hydrophobic Tails	52:78	Different Hydrophobic Tails for Plasmid DNA Delivery	52:103	Evaluation of Maltose-Based Cationic Liposomes with Different Hydrophobic Tails for Plasmid DNA Delivery.
28287501	5	56	theme	gene	1202:1205	arg1	efficiency					1216:1225	high gene transfer efficiency	1197:1225	high gene transfer efficiency	1197:1225	Importantly, it indicated that Malt-DiC14MA exhibited high gene transfer efficiency and better uptake capability at N/P ratios of 8:1.
28287501	2	57	theme	¹H-¹H	555:559	arg1	COSY					561:564	¹H-¹H COSY	555:564	¹H-¹H COSY	555:564	Target compounds and some intermediates were characterized by ¹H-NMR, 13C-NMR, ¹H-¹H COSY and ¹H-13C HSQC.
28287501	3	58	theme	images	649:654	arg1	results					587:593	The results	583:593	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS)	583:695	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	3	59	theme	assay	618:622	arg1	results					587:593	The results	583:593	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS)	583:695	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	4	60	theme	pEGFP-C1	1067:1074	arg1	images					1121:1126	Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images	1055:1126	Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images	1055:1126	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	1	61	theme	cationic	127:134	arg1	glycolipids					136:146	three cationic glycolipids	121:146	three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c)	121:245	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	4	62	theme	enhanced	998:1005	arg1	expression					1040:1049	the enhanced green fluorescent protein (EGFP) expression	994:1049	the enhanced green fluorescent protein (EGFP) expression	994:1049	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	1	63	theme	IX	217:218	arg1	b					220:220	IX b	217:220	IX b	217:220	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	1	63	theme	IX	217:218	arg1	Malt-DiC14MA					203:214	Malt-DiC14MA	203:214	Malt-DiC14MA (IX b)	203:221	In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA (IX a), Malt-DiC14MA (IX b) and Malt-DiC16MA (IX c) were constructed by using maltose as starting material via peracetylation, selective 1-O-deacetylation, trichloroacetimidation, glycosylation, azidation, deacetylation, Staudinger reaction, tertiary amination and quaternization.
28287501	6	64	theme	cell	1306:1309	arg1	viability					1311:1319	cell viability	1306:1319	cell viability	1306:1319	Additionally, the result of cell viability showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay.
28287501	4	65	theme	Cy3-labeled	1055:1065	arg1	images					1121:1126	Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images	1055:1126	Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images	1055:1126	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	6	66	theme	viability	1311:1319	arg1	result					1296:1301	the result	1292:1301	the result of cell viability	1292:1319	Additionally, the result of cell viability showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay.
28287501	6	67	theme	tetrazolium	1410:1420	arg1	bromide					1422:1428	blue tetrazolium bromide	1405:1428	thiazolyl blue tetrazolium bromide (MTT) assay	1395:1440	Additionally, the result of cell viability showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay.
28287501	6	67	theme	tetrazolium	1410:1420	arg1	MTT					1431:1433	MTT	1431:1433	MTT	1431:1433	Additionally, the result of cell viability showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay.
28287501	3	68	theme	compact	759:765	arg1	DNA					767:769	compact DNA	759:769	compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4)	759:855	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	6	69	theme	blue	1405:1408	arg1	bromide					1422:1428	blue tetrazolium bromide	1405:1428	thiazolyl blue tetrazolium bromide (MTT) assay	1395:1440	Additionally, the result of cell viability showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay.
28287501	6	69	theme	blue	1405:1408	arg1	MTT					1431:1433	MTT	1431:1433	MTT	1431:1433	Additionally, the result of cell viability showed glycolipids exhibited low biotoxicity and good biocompatibility by thiazolyl blue tetrazolium bromide (MTT) assay.
28287501	3	70	theme	light	674:678	arg1	DLS					692:694	DLS	692:694	DLS	692:694	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	3	70	theme	light	674:678	arg1	scattering					680:689	dynamic light scattering	666:689	dynamic light scattering (DLS)	666:695	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	3	71	theme	gel	598:600	arg1	assay					618:622	gel electrophoresis assay	598:622	gel electrophoresis assay	598:622	The results of gel electrophoresis assay, atomic force microscopy images (AFM) and dynamic light scattering (DLS) demonstrate that all the liposomes could efficiently bind and compact DNA (N/P ratio less than 2) into nanoparticles with proper size (88 nm-146 nm, PDI < 0.4) and zeta potential (+15 mV-+26 mV).
28287501	4	72	theme	Enhanced	1077:1084	arg1	Protein					1104:1110	Enhanced Green Fluorescent Protein	1077:1110	Enhanced Green Fluorescent Protein plasmid	1077:1118	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	4	73	from	cell	966:969	arg1	efficiency					910:919	transfection efficiency	897:919	transfection efficiency	897:919	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	4	73	from	cell	966:969	arg1	uptake					934:939	cellular uptake	925:939	cellular uptake	925:939	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28287501	4	74	theme	HEK293	959:964	arg1	cell					966:969	HEK293 cell	959:969	HEK293 cell	959:969	The transfection efficiency and cellular uptake of glycolipids in HEK293 cell were evaluated through the enhanced green fluorescent protein (EGFP) expression and Cy3-labeled pEGFP-C1 (Enhanced Green Fluorescent Protein plasmid) images, respectively.
28237569	6	0	from	application	870:880	arg1	field					889:893	the field	885:893	the field of synthetic wig	885:910	Herein, the SA/AKP fiber with superior mechanical and functional performance had practical value for application in the field of synthetic wig.
28237569	4	1	theme	excellent	634:642	arg1	hygroscopicity					644:657	excellent hygroscopicity	634:657	excellent hygroscopicity	634:657	Moreover, comparing with human hair, the SA/AKP fiber had a lot of unique groove upon the fiber surface, which contributed a lot to excellent hygroscopicity.
28237569	4	2	theme	SA/AKP	543:548	arg1	fiber					550:554	the SA/AKP fiber	539:554	the SA/AKP fiber	539:554	Moreover, comparing with human hair, the SA/AKP fiber had a lot of unique groove upon the fiber surface, which contributed a lot to excellent hygroscopicity.
28237569	0	3	theme	biomass	79:85	arg1	materials					87:95	biomass materials	79:95	biomass materials	79:95	Preparation and characterization of novel super-artificial hair fiber based on biomass materials.
28237569	6	4	theme	mechanical	808:817	arg1	performance					834:844	superior mechanical and functional performance	799:844	superior mechanical and functional performance	799:844	Herein, the SA/AKP fiber with superior mechanical and functional performance had practical value for application in the field of synthetic wig.
28237569	6	5	theme	practical	850:858	arg1	value					860:864	practical value	850:864	practical value for application in the field of synthetic wig	850:910	Herein, the SA/AKP fiber with superior mechanical and functional performance had practical value for application in the field of synthetic wig.
28237569	5	6	theme	dyeing	675:680	arg1	performance					682:692	the dyeing performance	671:692	the dyeing performance	671:692	Meanwhile, the dyeing performance could be improved notably due to incorporation of protein into the matrix.
28237569	5	6	theme	dyeing	675:680	arg1	Meanwhile					660:668	Meanwhile	660:668	Meanwhile	660:668	Meanwhile, the dyeing performance could be improved notably due to incorporation of protein into the matrix.
28237569	4	7	theme	groove	576:581	arg1	lot					562:564	a lot	560:564	a lot of unique groove	560:581	Moreover, comparing with human hair, the SA/AKP fiber had a lot of unique groove upon the fiber surface, which contributed a lot to excellent hygroscopicity.
28237569	4	7	theme	groove	576:581	arg1	groove					576:581	unique groove	569:581	unique groove	569:581	Moreover, comparing with human hair, the SA/AKP fiber had a lot of unique groove upon the fiber surface, which contributed a lot to excellent hygroscopicity.
28237569	1	8	theme	Antarctic	169:177	arg1	AKP					194:196	AKP	194:196	AKP	194:196	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	1	8	theme	Antarctic	169:177	arg1	protein					185:191	Antarctic Krill protein	169:191	Antarctic Krill protein (AKP)	169:197	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	5	9	theme	protein	744:750	arg1	incorporation					727:739	incorporation	727:739	incorporation of protein into the matrix	727:766	Meanwhile, the dyeing performance could be improved notably due to incorporation of protein into the matrix.
28237569	6	10	theme	functional	823:832	arg1	performance					834:844	superior mechanical and functional performance	799:844	superior mechanical and functional performance	799:844	Herein, the SA/AKP fiber with superior mechanical and functional performance had practical value for application in the field of synthetic wig.
28237569	2	11	theme	SA/AKP	247:252	arg1	fiber					254:258	Such SA/AKP fiber	242:258	Such SA/AKP fiber	242:258	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	6	12	with	fiber	788:792	arg1	performance					834:844	superior mechanical and functional performance	799:844	superior mechanical and functional performance	799:844	Herein, the SA/AKP fiber with superior mechanical and functional performance had practical value for application in the field of synthetic wig.
28237569	2	13	contain	had	334:336	arg2	performance					376:386	mechanical performance	365:386	mechanical performance	365:386	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	2	13	contain	had	334:336	arg1	fiber					254:258	Such SA/AKP fiber	242:258	Such SA/AKP fiber	242:258	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	2	13	contain	had	334:336	arg2	resistance					350:359	super flame resistance	338:359	super flame resistance	338:359	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	2	14	theme	crystalline	286:296	arg1	structure					298:306	similar crystalline structure	278:306	similar crystalline structure	278:306	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	2	15	theme	flame	344:348	arg1	resistance					350:359	super flame resistance	338:359	super flame resistance	338:359	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	4	16	theme	human	527:531	arg1	hair					533:536	human hair	527:536	human hair	527:536	Moreover, comparing with human hair, the SA/AKP fiber had a lot of unique groove upon the fiber surface, which contributed a lot to excellent hygroscopicity.
28237569	2	17	theme	similar	278:284	arg1	structure					298:306	similar crystalline structure	278:306	similar crystalline structure	278:306	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	2	18	theme	super	338:342	arg1	resistance					350:359	super flame resistance	338:359	super flame resistance	338:359	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	1	19	from	fiber	128:132	arg1	alginate					151:158	sodium alginate	144:158	sodium alginate (SA)	144:163	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	1	19	from	fiber	128:132	arg1	AKP					194:196	AKP	194:196	AKP	194:196	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	1	19	from	fiber	128:132	arg1	protein					185:191	Antarctic Krill protein	169:191	Antarctic Krill protein (AKP)	169:197	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	1	19	from	fiber	128:132	arg1	SA					161:162	SA	161:162	SA	161:162	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	1	20	theme	novel	100:104	arg1	fiber					128:132	A novel super-artificial hair fiber	98:132	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP)	98:197	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	3	21	theme	preparation	423:433	arg1	process					435:441	the whole preparation process	413:441	the whole preparation process	413:441	It should be noted that the whole preparation process was green without any incorporation of non-toxic solution.
28237569	6	22	theme	wig	908:910	arg1	field					889:893	the field	885:893	the field of synthetic wig	885:910	Herein, the SA/AKP fiber with superior mechanical and functional performance had practical value for application in the field of synthetic wig.
28237569	4	23	dep	had	556:558	arg1	contributed					613:623	contributed	613:623	contributed a lot to excellent hygroscopicity	613:657	Moreover, comparing with human hair, the SA/AKP fiber had a lot of unique groove upon the fiber surface, which contributed a lot to excellent hygroscopicity.
28237569	1	24	theme	super-artificial	106:121	arg1	fiber					128:132	A novel super-artificial hair fiber	98:132	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP)	98:197	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	3	25	theme	non-toxic	482:490	arg1	solution					492:499	non-toxic solution	482:499	non-toxic solution	482:499	It should be noted that the whole preparation process was green without any incorporation of non-toxic solution.
28237569	0	26	theme	super-artificial	42:57	arg1	fiber					64:68	novel super-artificial hair fiber	36:68	novel super-artificial hair fiber	36:68	Preparation and characterization of novel super-artificial hair fiber based on biomass materials.
28237569	6	27	theme	synthetic	898:906	arg1	wig					908:910	synthetic wig	898:910	synthetic wig	898:910	Herein, the SA/AKP fiber with superior mechanical and functional performance had practical value for application in the field of synthetic wig.
28237569	6	28	theme	superior	799:806	arg1	performance					834:844	superior mechanical and functional performance	799:844	superior mechanical and functional performance	799:844	Herein, the SA/AKP fiber with superior mechanical and functional performance had practical value for application in the field of synthetic wig.
28237569	1	29	theme	Krill	179:183	arg1	AKP					194:196	AKP	194:196	AKP	194:196	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	1	29	theme	Krill	179:183	arg1	protein					185:191	Antarctic Krill protein	169:191	Antarctic Krill protein (AKP)	169:197	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	4	30	theme	fiber	592:596	arg1	surface					598:604	the fiber surface	588:604	the fiber surface	588:604	Moreover, comparing with human hair, the SA/AKP fiber had a lot of unique groove upon the fiber surface, which contributed a lot to excellent hygroscopicity.
28237569	0	31	theme	novel	36:40	arg1	fiber					64:68	novel super-artificial hair fiber	36:68	novel super-artificial hair fiber	36:68	Preparation and characterization of novel super-artificial hair fiber based on biomass materials.
28237569	4	32	contain	had	556:558	arg1	fiber					550:554	the SA/AKP fiber	539:554	the SA/AKP fiber	539:554	Moreover, comparing with human hair, the SA/AKP fiber had a lot of unique groove upon the fiber surface, which contributed a lot to excellent hygroscopicity.
28237569	4	32	contain	had	556:558	arg2	groove					576:581	unique groove	569:581	unique groove	569:581	Moreover, comparing with human hair, the SA/AKP fiber had a lot of unique groove upon the fiber surface, which contributed a lot to excellent hygroscopicity.
28237569	4	32	contain	had	556:558	arg2	lot					562:564	a lot	560:564	a lot of unique groove	560:581	Moreover, comparing with human hair, the SA/AKP fiber had a lot of unique groove upon the fiber surface, which contributed a lot to excellent hygroscopicity.
28237569	2	33	theme	mechanical	365:374	arg1	performance					376:386	mechanical performance	365:386	mechanical performance	365:386	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	0	34	theme	fiber	64:68	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of novel super-artificial hair fiber based on biomass materials.
28237569	0	34	theme	fiber	64:68	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of novel super-artificial hair fiber based on biomass materials.
28237569	2	35	contain	have	273:276	arg2	structure					298:306	similar crystalline structure	278:306	similar crystalline structure	278:306	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	2	35	contain	have	273:276	arg1	fiber					254:258	Such SA/AKP fiber	242:258	Such SA/AKP fiber	242:258	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	1	36	theme	hair	123:126	arg1	fiber					128:132	A novel super-artificial hair fiber	98:132	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP)	98:197	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	0	37	theme	hair	59:62	arg1	fiber					64:68	novel super-artificial hair fiber	36:68	novel super-artificial hair fiber	36:68	Preparation and characterization of novel super-artificial hair fiber based on biomass materials.
28237569	6	38	theme	SA/AKP	781:786	arg1	fiber					788:792	the SA/AKP fiber	777:792	the SA/AKP fiber with superior mechanical and functional performance	777:844	Herein, the SA/AKP fiber with superior mechanical and functional performance had practical value for application in the field of synthetic wig.
28237569	2	39	theme	human	313:317	arg1	hair					319:322	human hair	313:322	human hair	313:322	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	2	40	theme	Such	242:245	arg1	fiber					254:258	Such SA/AKP fiber	242:258	Such SA/AKP fiber	242:258	Such SA/AKP fiber did not only have similar crystalline structure with human hair, but also had super flame resistance and mechanical performance.
28237569	6	41	contain	had	846:848	arg2	value					860:864	practical value	850:864	practical value for application in the field of synthetic wig	850:910	Herein, the SA/AKP fiber with superior mechanical and functional performance had practical value for application in the field of synthetic wig.
28237569	6	41	contain	had	846:848	arg1	fiber					788:792	the SA/AKP fiber	777:792	the SA/AKP fiber with superior mechanical and functional performance	777:844	Herein, the SA/AKP fiber with superior mechanical and functional performance had practical value for application in the field of synthetic wig.
28237569	3	42	theme	whole	417:421	arg1	process					435:441	the whole preparation process	413:441	the whole preparation process	413:441	It should be noted that the whole preparation process was green without any incorporation of non-toxic solution.
28237569	1	43	theme	sodium	144:149	arg1	alginate					151:158	sodium alginate	144:158	sodium alginate (SA)	144:163	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	1	43	theme	sodium	144:149	arg1	SA					161:162	SA	161:162	SA	161:162	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
28237569	4	44	theme	unique	569:574	arg1	groove					576:581	unique groove	569:581	unique groove	569:581	Moreover, comparing with human hair, the SA/AKP fiber had a lot of unique groove upon the fiber surface, which contributed a lot to excellent hygroscopicity.
28237569	3	45	theme	solution	492:499	arg1	incorporation					465:477	any incorporation	461:477	any incorporation of non-toxic solution	461:499	It should be noted that the whole preparation process was green without any incorporation of non-toxic solution.
28237569	1	46	theme	wet	215:217	arg1	spinning					219:226	wet spinning	215:226	wet spinning	215:226	A novel super-artificial hair fiber basing on sodium alginate (SA) and Antarctic Krill protein (AKP) was prepared by wet spinning successfully.
27065450	1	0	theme	Photosens	214:222	arg1	amounts					183:189	different amounts	173:189	different amounts of the photosensitizer Photosens	173:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	1	0	theme	Photosens	214:222	arg1	Photosens					214:222	the photosensitizer Photosens	194:222	the photosensitizer Photosens	194:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	1	1	theme	confocal	319:326	arg1	microscopy					343:352	confocal laser scanning microscopy	319:352	confocal laser scanning microscopy	319:352	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	6	2	theme	Photosens-containing	1099:1118	arg1	nanofibers					1141:1150	the Photosens-containing electrospun chitosan nanofibers	1095:1150	the Photosens-containing electrospun chitosan nanofibers described here	1095:1165	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	5	3	theme	Photosens	1007:1015	arg1	scaffolds					1029:1037	Photosens -containing scaffolds	1007:1037	Photosens -containing scaffolds	1007:1037	Interestingly, the noncancerous MC3T3-E1 cells grown on Photosens -containing scaffolds were less affected by the irradiation.
27065450	2	4	from	materials	389:397	arg1	release					359:365	The release	355:365	The release of Photosens from the materials	355:397	The release of Photosens from the materials was investigated in water and in phosphate-buffered saline.
27065450	1	5	theme	laser	328:332	arg1	microscopy					343:352	confocal laser scanning microscopy	319:352	confocal laser scanning microscopy	319:352	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	3	6	theme	mammary	528:534	arg1	[T-47D					520:525	a cancerous [T-47D	508:525	a cancerous [T-47D (mammary gland)	508:541	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	3	6	theme	mammary	528:534	arg1	gland					536:540	mammary gland	528:540	mammary gland	528:540	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	1	7	contain	containing	162:171	arg2	Photosens					214:222	the photosensitizer Photosens	194:222	the photosensitizer Photosens	194:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	1	7	contain	containing	162:171	arg2	amounts					183:189	different amounts	173:189	different amounts of the photosensitizer Photosens	173:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	1	7	contain	containing	162:171	arg1	materials					152:160	Novel chitosan-based nanofibrous composite materials	109:160	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens	109:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	3	8	dep	noncancerous	461:472	arg1	osteoblasts					491:501	MC3T3-E1 murine osteoblasts	475:501	MC3T3-E1 murine osteoblasts	475:501	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	3	9	theme	cell	609:612	arg1	growth					614:619	cell growth	609:619	cell growth	609:619	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	3	10	theme	metabolic	625:633	arg1	activity					635:642	metabolic activity	625:642	metabolic activity	625:642	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	1	11	theme	scanning	334:341	arg1	microscopy					343:352	confocal laser scanning microscopy	319:352	confocal laser scanning microscopy	319:352	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	4	12	theme	Photosens-containing	822:841	arg1	fibers					843:848	Photosens-containing fibers	822:848	Photosens-containing fibers	822:848	The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density.
27065450	6	13	theme	photodynamic	1243:1254	arg1	therapy					1256:1262	topical photodynamic therapy	1235:1262	topical photodynamic therapy	1235:1262	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	6	13	theme	photodynamic	1243:1254	arg1	applications					1208:1219	biomedical applications	1197:1219	biomedical applications	1197:1219	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	3	14	theme	cell	544:547	arg1	line					549:552	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line	459:552	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line	459:552	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	4	15	theme	density	942:948	arg1	irradiation					891:901	laser irradiation	885:901	laser irradiation of an appropriate wavelength and power density	885:948	The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density.
27065450	3	16	theme	cancerous	510:518	arg1	[T-47D					520:525	a cancerous [T-47D	508:525	a cancerous [T-47D (mammary gland)	508:541	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	3	16	theme	cancerous	510:518	arg1	gland					536:540	mammary gland	528:540	mammary gland	528:540	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	4	17	theme	power	936:940	arg1	density					942:948	power density	936:948	power density	936:948	The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density.
27065450	1	18	theme	Novel	109:113	arg1	materials					152:160	Novel chitosan-based nanofibrous composite materials	109:160	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens	109:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	6	19	theme	topical	1235:1241	arg1	therapy					1256:1262	topical photodynamic therapy	1235:1262	topical photodynamic therapy	1235:1262	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	6	19	theme	topical	1235:1241	arg1	applications					1208:1219	biomedical applications	1197:1219	biomedical applications	1197:1219	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	3	20	theme	[T-47D	520:525	arg1	line					549:552	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line	459:552	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line	459:552	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	3	21	theme	noncancerous	461:472	arg1	line					549:552	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line	459:552	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line	459:552	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	1	22	theme	chitosan-based	115:128	arg1	materials					152:160	Novel chitosan-based nanofibrous composite materials	109:160	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens	109:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	5	23	theme	noncancerous	970:981	arg1	cells					992:996	the noncancerous MC3T3-E1 cells	966:996	the noncancerous MC3T3-E1 cells grown on Photosens -containing scaffolds	966:1037	Interestingly, the noncancerous MC3T3-E1 cells grown on Photosens -containing scaffolds were less affected by the irradiation.
27065450	0	24	theme	Photosensitizer-loaded	0:21	arg1	electrospun					23:33	Photosensitizer-loaded electrospun	0:33	Photosensitizer-loaded electrospun	0:33	Photosensitizer-loaded electrospun chitosan-based scaffolds for photodynamic therapy and tissue engineering.
27065450	4	25	theme	cell	808:811	arg1	lines					813:817	both cell lines	803:817	both cell lines	803:817	The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density.
27065450	1	26	theme	nanofibrous	130:140	arg1	materials					152:160	Novel chitosan-based nanofibrous composite materials	109:160	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens	109:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	6	27	theme	chitosan	1132:1139	arg1	nanofibers					1141:1150	the Photosens-containing electrospun chitosan nanofibers	1095:1150	the Photosens-containing electrospun chitosan nanofibers described here	1095:1165	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	5	28	theme	MC3T3-E1	983:990	arg1	cells					992:996	the noncancerous MC3T3-E1 cells	966:996	the noncancerous MC3T3-E1 cells grown on Photosens -containing scaffolds	966:1037	Interestingly, the noncancerous MC3T3-E1 cells grown on Photosens -containing scaffolds were less affected by the irradiation.
27065450	1	29	theme	composite	142:150	arg1	materials					152:160	Novel chitosan-based nanofibrous composite materials	109:160	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens	109:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	4	30	theme	appropriate	909:919	arg1	wavelength					921:930	an appropriate wavelength	906:930	an appropriate wavelength	906:930	The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density.
27065450	6	31	theme	tissue	1268:1273	arg1	engineering					1275:1285	tissue engineering	1268:1285	tissue engineering	1268:1285	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	6	31	theme	tissue	1268:1273	arg1	applications					1208:1219	biomedical applications	1197:1219	biomedical applications	1197:1219	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	3	32	theme	4,5-dimethylthiazol-2-yl	706:729	arg1	assay					765:769	3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay	703:769	3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay	703:769	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	2	33	theme	Photosens	370:378	arg1	release					359:365	The release	355:365	The release of Photosens from the materials	355:397	The release of Photosens from the materials was investigated in water and in phosphate-buffered saline.
27065450	0	34	theme	photodynamic	64:75	arg1	therapy					77:83	photodynamic therapy	64:83	photodynamic therapy	64:83	Photosensitizer-loaded electrospun chitosan-based scaffolds for photodynamic therapy and tissue engineering.
27065450	3	35	theme	confocal	661:668	arg1	microscopy					685:694	confocal laser scanning microscopy	661:694	confocal laser scanning microscopy	661:694	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	6	36	theme	biomedical	1197:1206	arg1	therapy					1256:1262	topical photodynamic therapy	1235:1262	topical photodynamic therapy	1235:1262	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	6	36	theme	biomedical	1197:1206	arg1	applications					1208:1219	biomedical applications	1197:1219	biomedical applications	1197:1219	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	6	36	theme	biomedical	1197:1206	arg1	engineering					1275:1285	tissue engineering	1268:1285	tissue engineering	1268:1285	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	6	37	theme	potential	1174:1182	arg1	interest					1184:1191	potential interest	1174:1191	potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering	1174:1285	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	6	38	theme	electrospun	1120:1130	arg1	nanofibers					1141:1150	the Photosens-containing electrospun chitosan nanofibers	1095:1150	the Photosens-containing electrospun chitosan nanofibers described here	1095:1165	We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.
27065450	3	39	theme	laser	670:674	arg1	microscopy					685:694	confocal laser scanning microscopy	661:694	confocal laser scanning microscopy	661:694	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	1	40	theme	scanning	283:290	arg1	microscopy					301:310	scanning electron microscopy	283:310	scanning electron microscopy	283:310	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	3	41	theme	MC3T3-E1	475:482	arg1	osteoblasts					491:501	MC3T3-E1 murine osteoblasts	475:501	MC3T3-E1 murine osteoblasts	475:501	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	5	42	theme	-containing	1017:1027	arg1	scaffolds					1029:1037	Photosens -containing scaffolds	1007:1037	Photosens -containing scaffolds	1007:1037	Interestingly, the noncancerous MC3T3-E1 cells grown on Photosens -containing scaffolds were less affected by the irradiation.
27065450	3	43	theme	murine	484:489	arg1	osteoblasts					491:501	MC3T3-E1 murine osteoblasts	475:501	MC3T3-E1 murine osteoblasts	475:501	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	4	44	theme	wavelength	921:930	arg1	irradiation					891:901	laser irradiation	885:901	laser irradiation of an appropriate wavelength and power density	885:948	The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density.
27065450	1	45	theme	different	173:181	arg1	amounts					183:189	different amounts	173:189	different amounts of the photosensitizer Photosens	173:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	1	45	theme	different	173:181	arg1	Photosens					214:222	the photosensitizer Photosens	194:222	the photosensitizer Photosens	194:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	4	46	theme	laser	885:889	arg1	irradiation					891:901	laser irradiation	885:901	laser irradiation of an appropriate wavelength and power density	885:948	The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density.
27065450	4	47	theme	lines	813:817	arg1	viability					790:798	The viability	786:798	The viability of both cell lines on Photosens-containing fibers	786:848	The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density.
27065450	1	48	theme	electron	292:299	arg1	microscopy					301:310	scanning electron microscopy	283:310	scanning electron microscopy	283:310	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	4	49	theme	spatial	865:871	arg1	manner					873:878	a spatial manner	863:878	a spatial manner	863:878	The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density.
27065450	3	50	theme	-2,5-diphe-nyltetrazolium	731:755	arg1	assay					765:769	3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay	703:769	3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay	703:769	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	1	51	theme	photosensitizer	198:212	arg1	Photosens					214:222	the photosensitizer Photosens	194:222	the photosensitizer Photosens	194:222	Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy.
27065450	3	52	theme	bromide	757:763	arg1	assay					765:769	3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay	703:769	3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay	703:769	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	3	53	theme	scanning	676:683	arg1	microscopy					685:694	confocal laser scanning microscopy	661:694	confocal laser scanning microscopy	661:694	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	3	54	theme	Photosens-containing	573:592	arg1	scaffolds					594:602	Photosens-containing scaffolds	573:602	Photosens-containing scaffolds	573:602	A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively.
27065450	0	55	theme	tissue	89:94	arg1	engineering					96:106	tissue engineering	89:106	tissue engineering	89:106	Photosensitizer-loaded electrospun chitosan-based scaffolds for photodynamic therapy and tissue engineering.
27065450	2	56	theme	phosphate-buffered	432:449	arg1	saline					451:456	phosphate-buffered saline	432:456	phosphate-buffered saline	432:456	The release of Photosens from the materials was investigated in water and in phosphate-buffered saline.
27065450	4	57	from	viability	790:798	arg1	fibers					843:848	Photosens-containing fibers	822:848	Photosens-containing fibers	822:848	The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density.
29246871	7	0	theme	new	1097:1099	arg1	bone					1108:1111	new mature bone	1097:1111	new mature bone	1097:1111	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	3	1	theme	suitable	562:569	arg1	injectability					571:583	a suitable injectability	560:583	a suitable injectability	560:583	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	1	2	theme	precursor	263:271	arg1	salts					273:277	the precursor salts	259:277	the precursor salts	259:277	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	4	3	theme	MC	717:718	arg1	NPs					744:746	the MC hydrogel containing CaP NPs	713:746	the MC hydrogel containing CaP NPs	713:746	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	4	4	theme	physiological	668:680	arg1	condition					685:693	the physiological pH condition	664:693	the physiological pH condition	664:693	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	7	5	theme	MC-HAP	1136:1141	arg1	hydrogel					1157:1164	the MC-HAP NPs composite hydrogel	1132:1164	the MC-HAP NPs composite hydrogel	1132:1164	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	5	6	theme	in	853:854	arg1	NPs					877:879	the in situ synthesized CaP NPs	849:879	the in situ synthesized CaP NPs	849:879	The XRD results indicate that the in situ synthesized CaP NPs had a crystalline phase of hydroxyapatite (HAP).
29246871	3	7	theme	body	592:595	arg1	temperature					597:607	the body temperature	588:607	the body temperature	588:607	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	5	8	theme	synthesized	861:871	arg1	NPs					877:879	the in situ synthesized CaP NPs	849:879	the in situ synthesized CaP NPs	849:879	The XRD results indicate that the in situ synthesized CaP NPs had a crystalline phase of hydroxyapatite (HAP).
29246871	8	9	contain	has	1277:1279	arg1	hydrogel					1268:1275	injectable MC-HAP NPs composite hydrogel	1236:1275	injectable MC-HAP NPs composite hydrogel	1236:1275	The results of this study indicate that injectable MC-HAP NPs composite hydrogel has a great potential for bone tissue regeneration.
29246871	8	9	contain	has	1277:1279	arg2	potential					1289:1297	a great potential	1281:1297	a great potential for bone tissue regeneration	1281:1326	The results of this study indicate that injectable MC-HAP NPs composite hydrogel has a great potential for bone tissue regeneration.
29246871	8	10	theme	tissue	1308:1313	arg1	regeneration					1315:1326	bone tissue regeneration	1303:1326	bone tissue regeneration	1303:1326	The results of this study indicate that injectable MC-HAP NPs composite hydrogel has a great potential for bone tissue regeneration.
29246871	1	11	theme	in	223:224	arg1	process					241:247	an in situ formation process	220:247	an in situ formation process	220:247	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	8	12	theme	injectable	1236:1245	arg1	hydrogel					1268:1275	injectable MC-HAP NPs composite hydrogel	1236:1275	injectable MC-HAP NPs composite hydrogel	1236:1275	The results of this study indicate that injectable MC-HAP NPs composite hydrogel has a great potential for bone tissue regeneration.
29246871	8	13	theme	NPs	1254:1256	arg1	hydrogel					1268:1275	injectable MC-HAP NPs composite hydrogel	1236:1275	injectable MC-HAP NPs composite hydrogel	1236:1275	The results of this study indicate that injectable MC-HAP NPs composite hydrogel has a great potential for bone tissue regeneration.
29246871	5	14	theme	crystalline	887:897	arg1	phase					899:903	a crystalline phase	885:903	a crystalline phase of hydroxyapatite (HAP)	885:927	The XRD results indicate that the in situ synthesized CaP NPs had a crystalline phase of hydroxyapatite (HAP).
29246871	5	15	theme	hydroxyapatite	908:921	arg1	phase					899:903	a crystalline phase	885:903	a crystalline phase of hydroxyapatite (HAP)	885:927	The XRD results indicate that the in situ synthesized CaP NPs had a crystalline phase of hydroxyapatite (HAP).
29246871	6	16	theme	MC-HAP	990:995	arg1	hydrogel					1011:1018	MC-HAP NPs composite hydrogel	990:1018	MC-HAP NPs composite hydrogel	990:1018	The in vitro study using mesenchymal stem cells showed that MC-HAP NPs composite hydrogel was biocompatible.
29246871	5	17	contain	had	881:883	arg2	phase					899:903	a crystalline phase	885:903	a crystalline phase of hydroxyapatite (HAP)	885:927	The XRD results indicate that the in situ synthesized CaP NPs had a crystalline phase of hydroxyapatite (HAP).
29246871	5	17	contain	had	881:883	arg1	NPs					877:879	the in situ synthesized CaP NPs	849:879	the in situ synthesized CaP NPs	849:879	The XRD results indicate that the in situ synthesized CaP NPs had a crystalline phase of hydroxyapatite (HAP).
29246871	7	18	theme	MC	1183:1184	arg1	hydrogel					1186:1193	the pure MC hydrogel	1174:1193	the pure MC hydrogel	1174:1193	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	3	19	theme	crystalline	630:640	arg1	phases					642:647	the crystalline phases	626:647	the crystalline phases of CaP NPs	626:658	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	1	20	theme	phosphate	170:178	arg1	nanoparticles					180:192	calcium phosphate nanoparticles	162:192	calcium phosphate nanoparticles (CaP NPs)	162:202	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	1	20	theme	phosphate	170:178	arg1	NPs					199:201	CaP NPs	195:201	CaP NPs	195:201	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	6	21	theme	stem	967:970	arg1	cells					972:976	mesenchymal stem cells	955:976	mesenchymal stem cells	955:976	The in vitro study using mesenchymal stem cells showed that MC-HAP NPs composite hydrogel was biocompatible.
29246871	4	22	theme	scanning	764:771	arg1	diffraction					800:810	X-ray diffraction	794:810	X-ray diffraction (XRD)	794:816	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	4	22	theme	scanning	764:771	arg1	microscopy					782:791	scanning electron microscopy	764:791	scanning electron microscopy	764:791	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	4	23	theme	containing	729:738	arg1	NPs					744:746	the MC hydrogel containing CaP NPs	713:746	the MC hydrogel containing CaP NPs	713:746	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	8	24	theme	MC-HAP	1247:1252	arg1	hydrogel					1268:1275	injectable MC-HAP NPs composite hydrogel	1236:1275	injectable MC-HAP NPs composite hydrogel	1236:1275	The results of this study indicate that injectable MC-HAP NPs composite hydrogel has a great potential for bone tissue regeneration.
29246871	8	25	theme	bone	1303:1306	arg1	regeneration					1315:1326	bone tissue regeneration	1303:1326	bone tissue regeneration	1303:1326	The results of this study indicate that injectable MC-HAP NPs composite hydrogel has a great potential for bone tissue regeneration.
29246871	2	26	theme	different	396:404	arg1	phases					418:423	different crystalline phases	396:423	different crystalline phases	396:423	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases were characterized by rheometry, infrared spectroscopy and injectability test.
29246871	7	27	theme	composite	1147:1155	arg1	hydrogel					1157:1164	the MC-HAP NPs composite hydrogel	1132:1164	the MC-HAP NPs composite hydrogel	1132:1164	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	2	28	theme	infrared	458:465	arg1	spectroscopy					467:478	infrared spectroscopy	458:478	infrared spectroscopy	458:478	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases were characterized by rheometry, infrared spectroscopy and injectability test.
29246871	4	29	theme	NPs	744:746	arg1	structure					700:708	the structure	696:708	the structure of the MC hydrogel containing CaP NPs	696:746	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	2	30	theme	hydrogels	381:389	arg1	properties					346:355	The thermo-sensitive properties	325:355	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases	325:423	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases were characterized by rheometry, infrared spectroscopy and injectability test.
29246871	0	31	theme	Injectable	0:9	arg1	hydrogel					27:34	Injectable methylcellulose hydrogel	0:34	Injectable methylcellulose hydrogel containing calcium phosphate	0:63	Injectable methylcellulose hydrogel containing calcium phosphate nanoparticles for bone regeneration.
29246871	1	32	theme	injectable	110:119	arg1	hydrogel					142:149	A novel injectable methylcellulose (MC) hydrogel	102:149	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs)	102:202	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	5	33	theme	CaP	873:875	arg1	NPs					877:879	the in situ synthesized CaP NPs	849:879	the in situ synthesized CaP NPs	849:879	The XRD results indicate that the in situ synthesized CaP NPs had a crystalline phase of hydroxyapatite (HAP).
29246871	0	34	theme	bone	83:86	arg1	regeneration					88:99	bone regeneration	83:99	bone regeneration	83:99	Injectable methylcellulose hydrogel containing calcium phosphate nanoparticles for bone regeneration.
29246871	0	35	theme	calcium	47:53	arg1	phosphate					55:63	calcium phosphate	47:63	calcium phosphate	47:63	Injectable methylcellulose hydrogel containing calcium phosphate nanoparticles for bone regeneration.
29246871	6	36	theme	composite	1001:1009	arg1	hydrogel					1011:1018	MC-HAP NPs composite hydrogel	990:1018	MC-HAP NPs composite hydrogel	990:1018	The in vitro study using mesenchymal stem cells showed that MC-HAP NPs composite hydrogel was biocompatible.
29246871	3	37	theme	NPs	656:658	arg1	phases					642:647	the crystalline phases	626:647	the crystalline phases of CaP NPs	626:658	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	2	38	theme	thermo-sensitive	329:344	arg1	properties					346:355	The thermo-sensitive properties	325:355	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases	325:423	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases were characterized by rheometry, infrared spectroscopy and injectability test.
29246871	7	39	theme	bone	1108:1111	arg1	rate					1089:1092	the regeneration rate	1072:1092	the regeneration rate of new mature bone	1072:1111	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	7	39	theme	bone	1108:1111	arg1	higher					1122:1127	higher	1122:1127	higher	1122:1127	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	4	40	theme	X-ray	794:798	arg1	diffraction					800:810	X-ray diffraction	794:810	X-ray diffraction (XRD)	794:816	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	4	40	theme	X-ray	794:798	arg1	XRD					813:815	XRD	813:815	XRD	813:815	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	4	40	theme	X-ray	794:798	arg1	microscopy					782:791	scanning electron microscopy	764:791	scanning electron microscopy	764:791	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	3	41	theme	bioactive	538:546	arg1	NPs					552:554	bioactive CaP NPs	538:554	bioactive CaP NPs	538:554	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	2	42	with	hydrogels	381:389	arg1	phases					418:423	different crystalline phases	396:423	different crystalline phases	396:423	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases were characterized by rheometry, infrared spectroscopy and injectability test.
29246871	7	43	dep	in	1043:1044	arg1	vivo					1046:1049	vivo	1046:1049	vivo	1046:1049	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	3	44	with	hydrogels	523:531	arg1	NPs					552:554	bioactive CaP NPs	538:554	bioactive CaP NPs	538:554	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	7	45	theme	regeneration	1076:1087	arg1	rate					1089:1092	the regeneration rate	1072:1092	the regeneration rate of new mature bone	1072:1111	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	7	45	theme	regeneration	1076:1087	arg1	higher					1122:1127	higher	1122:1127	higher	1122:1127	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	3	46	theme	as-prepared	508:518	arg1	hydrogels					523:531	The as-prepared MC hydrogels	504:531	The as-prepared MC hydrogels with bioactive CaP NPs	504:554	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	1	47	theme	CaP	195:197	arg1	nanoparticles					180:192	calcium phosphate nanoparticles	162:192	calcium phosphate nanoparticles (CaP NPs)	162:202	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	1	47	theme	CaP	195:197	arg1	NPs					199:201	CaP NPs	195:201	CaP NPs	195:201	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	0	48	contain	containing	36:45	arg1	hydrogel					27:34	Injectable methylcellulose hydrogel	0:34	Injectable methylcellulose hydrogel containing calcium phosphate	0:63	Injectable methylcellulose hydrogel containing calcium phosphate nanoparticles for bone regeneration.
29246871	0	48	contain	containing	36:45	arg2	phosphate					55:63	calcium phosphate	47:63	calcium phosphate	47:63	Injectable methylcellulose hydrogel containing calcium phosphate nanoparticles for bone regeneration.
29246871	4	49	theme	pH	682:683	arg1	condition					685:693	the physiological pH condition	664:693	the physiological pH condition	664:693	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	7	50	theme	in	1043:1044	arg1	study					1051:1055	The in vivo study	1039:1055	The in vivo study	1039:1055	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	5	51	theme	XRD	823:825	arg1	results					827:833	The XRD results	819:833	The XRD results	819:833	The XRD results indicate that the in situ synthesized CaP NPs had a crystalline phase of hydroxyapatite (HAP).
29246871	6	52	theme	in	934:935	arg1	study					943:947	The in vitro study	930:947	The in vitro study using mesenchymal stem cells	930:976	The in vitro study using mesenchymal stem cells showed that MC-HAP NPs composite hydrogel was biocompatible.
29246871	3	53	theme	irrespective	610:621	arg1	temperature					597:607	the body temperature	588:607	the body temperature	588:607	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	8	54	theme	study	1216:1220	arg1	results					1200:1206	The results	1196:1206	The results of this study	1196:1220	The results of this study indicate that injectable MC-HAP NPs composite hydrogel has a great potential for bone tissue regeneration.
29246871	1	55	theme	MC	138:139	arg1	hydrogel					142:149	A novel injectable methylcellulose (MC) hydrogel	102:149	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs)	102:202	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	6	56	dep	in	934:935	arg1	vitro					937:941	vitro	937:941	vitro	937:941	The in vitro study using mesenchymal stem cells showed that MC-HAP NPs composite hydrogel was biocompatible.
29246871	3	57	theme	phases	642:647	arg1	irrespective					610:621	irrespective	610:621	irrespective	610:621	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	6	58	theme	NPs	997:999	arg1	hydrogel					1011:1018	MC-HAP NPs composite hydrogel	990:1018	MC-HAP NPs composite hydrogel	990:1018	The in vitro study using mesenchymal stem cells showed that MC-HAP NPs composite hydrogel was biocompatible.
29246871	4	59	theme	hydrogel	720:727	arg1	NPs					744:746	the MC hydrogel containing CaP NPs	713:746	the MC hydrogel containing CaP NPs	713:746	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	7	60	theme	pure	1178:1181	arg1	hydrogel					1186:1193	the pure MC hydrogel	1174:1193	the pure MC hydrogel	1174:1193	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	1	61	theme	calcium	162:168	arg1	phosphate					170:178	calcium phosphate	162:178	calcium phosphate nanoparticles (CaP NPs)	162:202	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	8	62	theme	composite	1258:1266	arg1	hydrogel					1268:1275	injectable MC-HAP NPs composite hydrogel	1236:1275	injectable MC-HAP NPs composite hydrogel	1236:1275	The results of this study indicate that injectable MC-HAP NPs composite hydrogel has a great potential for bone tissue regeneration.
29246871	6	63	theme	mesenchymal	955:965	arg1	cells					972:976	mesenchymal stem cells	955:976	mesenchymal stem cells	955:976	The in vitro study using mesenchymal stem cells showed that MC-HAP NPs composite hydrogel was biocompatible.
29246871	8	64	theme	great	1283:1287	arg1	potential					1289:1297	a great potential	1281:1297	a great potential for bone tissue regeneration	1281:1326	The results of this study indicate that injectable MC-HAP NPs composite hydrogel has a great potential for bone tissue regeneration.
29246871	7	65	theme	NPs	1143:1145	arg1	hydrogel					1157:1164	the MC-HAP NPs composite hydrogel	1132:1164	the MC-HAP NPs composite hydrogel	1132:1164	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	4	66	theme	electron	773:780	arg1	diffraction					800:810	X-ray diffraction	794:810	X-ray diffraction (XRD)	794:816	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	4	66	theme	electron	773:780	arg1	microscopy					782:791	scanning electron microscopy	764:791	scanning electron microscopy	764:791	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	0	67	theme	methylcellulose	11:25	arg1	hydrogel					27:34	Injectable methylcellulose hydrogel	0:34	Injectable methylcellulose hydrogel containing calcium phosphate	0:63	Injectable methylcellulose hydrogel containing calcium phosphate nanoparticles for bone regeneration.
29246871	1	68	theme	novel	104:108	arg1	hydrogel					142:149	A novel injectable methylcellulose (MC) hydrogel	102:149	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs)	102:202	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	4	69	theme	CaP	740:742	arg1	NPs					744:746	the MC hydrogel containing CaP NPs	713:746	the MC hydrogel containing CaP NPs	713:746	At the physiological pH condition, the structure of the MC hydrogel containing CaP NPs was analyzed by scanning electron microscopy, X-ray diffraction (XRD).
29246871	2	70	theme	composite	371:379	arg1	hydrogels					381:389	MC-CaP NPs composite hydrogels	360:389	MC-CaP NPs composite hydrogels with different crystalline phases	360:423	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases were characterized by rheometry, infrared spectroscopy and injectability test.
29246871	1	71	theme	methylcellulose	121:135	arg1	hydrogel					142:149	A novel injectable methylcellulose (MC) hydrogel	102:149	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs)	102:202	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	2	72	theme	NPs	367:369	arg1	hydrogels					381:389	MC-CaP NPs composite hydrogels	360:389	MC-CaP NPs composite hydrogels with different crystalline phases	360:423	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases were characterized by rheometry, infrared spectroscopy and injectability test.
29246871	1	73	theme	salt-out	289:296	arg1	effect					298:303	a salt-out effect	287:303	a salt-out effect	287:303	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	2	74	theme	injectability	484:496	arg1	test					498:501	injectability test	484:501	injectability test	484:501	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases were characterized by rheometry, infrared spectroscopy and injectability test.
29246871	3	75	contain	had	556:558	arg2	injectability					571:583	a suitable injectability	560:583	a suitable injectability	560:583	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	3	75	contain	had	556:558	arg1	hydrogels					523:531	The as-prepared MC hydrogels	504:531	The as-prepared MC hydrogels with bioactive CaP NPs	504:554	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	3	76	theme	CaP	652:654	arg1	NPs					656:658	CaP NPs	652:658	CaP NPs	652:658	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	2	77	theme	crystalline	406:416	arg1	phases					418:423	different crystalline phases	396:423	different crystalline phases	396:423	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases were characterized by rheometry, infrared spectroscopy and injectability test.
29246871	7	78	theme	mature	1101:1106	arg1	bone					1108:1111	new mature bone	1097:1111	new mature bone	1097:1111	The in vivo study indicated that the regeneration rate of new mature bone was also higher in the MC-HAP NPs composite hydrogel than in the pure MC hydrogel.
29246871	5	79	dep	in	853:854	arg1	situ					856:859	situ	856:859	situ	856:859	The XRD results indicate that the in situ synthesized CaP NPs had a crystalline phase of hydroxyapatite (HAP).
29246871	3	80	theme	MC	520:521	arg1	hydrogels					523:531	The as-prepared MC hydrogels	504:531	The as-prepared MC hydrogels with bioactive CaP NPs	504:554	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
29246871	1	81	theme	MC	312:313	arg1	solution					315:322	the MC solution	308:322	the MC solution	308:322	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	1	82	dep	in	223:224	arg1	situ					226:229	situ	226:229	situ	226:229	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	2	83	theme	MC-CaP	360:365	arg1	hydrogels					381:389	MC-CaP NPs composite hydrogels	360:389	MC-CaP NPs composite hydrogels with different crystalline phases	360:423	The thermo-sensitive properties of MC-CaP NPs composite hydrogels with different crystalline phases were characterized by rheometry, infrared spectroscopy and injectability test.
29246871	1	84	theme	formation	231:239	arg1	process					241:247	an in situ formation process	220:247	an in situ formation process	220:247	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	1	85	contain	containing	151:160	arg1	hydrogel					142:149	A novel injectable methylcellulose (MC) hydrogel	102:149	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs)	102:202	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	1	85	contain	containing	151:160	arg2	nanoparticles					180:192	calcium phosphate nanoparticles	162:192	calcium phosphate nanoparticles (CaP NPs)	162:202	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	1	85	contain	containing	151:160	arg2	NPs					199:201	CaP NPs	195:201	CaP NPs	195:201	A novel injectable methylcellulose (MC) hydrogel containing calcium phosphate nanoparticles (CaP NPs) was prepared by an in situ formation process, in which the precursor salts induced a salt-out effect in the MC solution.
29246871	3	86	theme	CaP	548:550	arg1	NPs					552:554	bioactive CaP NPs	538:554	bioactive CaP NPs	538:554	The as-prepared MC hydrogels with bioactive CaP NPs had a suitable injectability at the body temperature, irrespective of the crystalline phases of CaP NPs.
26511757	2	0	from	weaning	422:428	arg1	acids					401:405	dietary n-3 long-chain polyunsaturated fatty acids	356:405	dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning	356:428	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	0	from	weaning	422:428	arg1	PUFAs					412:416	n-3 PUFAs	408:416	n-3 PUFAs	408:416	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	0	1	theme	post-natal	160:169	arg1	life					171:174	pre- and post-natal life	151:174	pre- and post-natal life	151:174	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	2	2	theme	n-3	364:366	arg1	acids					401:405	dietary n-3 long-chain polyunsaturated fatty acids	356:405	dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning	356:428	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	2	theme	n-3	364:366	arg1	PUFAs					412:416	n-3 PUFAs	408:416	n-3 PUFAs	408:416	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	6	3	theme	enzyme	1259:1264	arg1	activity					1266:1273	increased lipogenic enzyme activity	1239:1273	increased lipogenic enzyme activity caused by a maternal SRD	1239:1298	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	2	4	theme	corn	575:578	arg1	oil					580:582	corn oil	575:582	corn oil	575:582	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	4	theme	corn	575:578	arg1	diet					540:543	a sucrose-rich diet	525:543	a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood	525:627	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	4	theme	corn	575:578	arg1	source					565:570	the fat source	557:570	the fat source	557:570	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	8	5	theme	breast	1639:1644	arg1	milk					1646:1649	SRD-dams' breast milk	1629:1649	SRD-dams' breast milk	1629:1649	SRD-dams' breast milk showed a more saturated fatty acid composition.
26511757	6	6	theme	activity	1337:1344	arg1	content					1474:1480	limited body fat content	1457:1480	limited body fat content	1457:1480	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	6	theme	activity	1337:1344	arg1	normalization					1314:1326	the complete normalization	1301:1326	the complete normalization of CPT I activity and PPARα protein mass levels	1301:1374	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	6	theme	activity	1337:1344	arg1	prevention					1169:1178	prevention	1169:1178	prevention	1169:1178	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	6	theme	activity	1337:1344	arg1	accretion					1420:1428	a slight but not statistically significant accretion	1377:1428	a slight but not statistically significant accretion of visceral adiposity	1377:1450	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	6	theme	activity	1337:1344	arg1	following					1158:1166	the following	1154:1166	the following	1154:1166	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	5	7	theme	RD	1039:1040	arg1	up					1042:1043	RD up	1039:1043	RD up to the end of the experimental period	1039:1081	Male offspring from RD-dams continued with RD up to the end of the experimental period, forming an RD-RD group.
26511757	4	8	theme	life	770:773	arg1	days					762:765	150 days	758:765	150 days of life	758:773	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	6	9	theme	CPT	1331:1333	arg1	activity					1337:1344	CPT I activity	1331:1344	CPT I activity	1331:1344	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	4	10	theme	male	776:779	arg1	offspring					781:789	male offspring	776:789	male offspring from SRD-dams	776:803	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	3	11	theme	powdered	699:706	arg1	diet					726:729	the standard powdered rodent commercial diet	686:729	the standard powdered rodent commercial diet (RD)	686:734	During pregnancy and lactation, dams were fed an SRD or the standard powdered rodent commercial diet (RD).
26511757	3	11	theme	powdered	699:706	arg1	RD					732:733	RD	732:733	RD	732:733	During pregnancy and lactation, dams were fed an SRD or the standard powdered rodent commercial diet (RD).
26511757	9	12	theme	adverse	1764:1770	arg1	outcomes					1772:1779	some adverse outcomes	1759:1779	some adverse outcomes induced by a maternal and post-weaning sucrose-rich diet	1759:1836	These results suggest the capacity of n-3 PUFAs to overcome some adverse outcomes induced by a maternal and post-weaning sucrose-rich diet.
26511757	8	13	theme	fatty	1675:1679	arg1	composition					1686:1696	a more saturated fatty acid composition	1658:1696	a more saturated fatty acid composition	1658:1696	SRD-dams' breast milk showed a more saturated fatty acid composition.
26511757	6	14	theme	slight	1379:1384	arg1	accretion					1420:1428	a slight but not statistically significant accretion	1377:1428	a slight but not statistically significant accretion of visceral adiposity	1377:1450	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	15	theme	fat	1470:1472	arg1	content					1474:1480	limited body fat content	1457:1480	limited body fat content	1457:1480	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	3	16	theme	commercial	715:724	arg1	diet					726:729	the standard powdered rodent commercial diet	686:729	the standard powdered rodent commercial diet (RD)	686:734	During pregnancy and lactation, dams were fed an SRD or the standard powdered rodent commercial diet (RD).
26511757	3	16	theme	commercial	715:724	arg1	RD					732:733	RD	732:733	RD	732:733	During pregnancy and lactation, dams were fed an SRD or the standard powdered rodent commercial diet (RD).
26511757	4	17	dep	CO	953:954	arg1	%					958:958	1%	957:958	1%	957:958	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	6	18	theme	limited	1457:1463	arg1	content					1474:1480	limited body fat content	1457:1480	limited body fat content	1457:1480	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	9	19	theme	PUFAs	1741:1745	arg1	capacity					1725:1732	the capacity	1721:1732	the capacity of n-3 PUFAs to overcome some adverse outcomes induced by a maternal and post-weaning sucrose-rich diet	1721:1836	These results suggest the capacity of n-3 PUFAs to overcome some adverse outcomes induced by a maternal and post-weaning sucrose-rich diet.
26511757	0	20	theme	pre-	151:154	arg1	life					171:174	pre- and post-natal life	151:174	pre- and post-natal life	151:174	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	6	21	theme	mass	1364:1367	arg1	levels					1369:1374	PPARα protein mass levels	1350:1374	PPARα protein mass levels	1350:1374	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	4	22	theme	SRD-SRD	970:976	arg1	groups					988:993	SRD-SRD or SRD-FO groups	970:993	SRD-SRD or SRD-FO groups	970:993	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	5	23	theme	RD-RD	1095:1099	arg1	group					1101:1105	an RD-RD group	1092:1105	an RD-RD group	1092:1105	Male offspring from RD-dams continued with RD up to the end of the experimental period, forming an RD-RD group.
26511757	6	24	theme	PPARα	1350:1354	arg1	levels					1369:1374	PPARα protein mass levels	1350:1374	PPARα protein mass levels	1350:1374	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	4	25	theme	SRD-FO	981:986	arg1	groups					988:993	SRD-SRD or SRD-FO groups	970:993	SRD-SRD or SRD-FO groups	970:993	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	0	26	theme	dyslipidemia	72:83	arg1	prevention					58:67	prevention	58:67	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.	0:175	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	6	27	theme	adiposity	1442:1450	arg1	content					1474:1480	limited body fat content	1457:1480	limited body fat content	1457:1480	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	27	theme	adiposity	1442:1450	arg1	normalization					1314:1326	the complete normalization	1301:1326	the complete normalization of CPT I activity and PPARα protein mass levels	1301:1374	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	27	theme	adiposity	1442:1450	arg1	prevention					1169:1178	prevention	1169:1178	prevention	1169:1178	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	27	theme	adiposity	1442:1450	arg1	accretion					1420:1428	a slight but not statistically significant accretion	1377:1428	a slight but not statistically significant accretion of visceral adiposity	1377:1450	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	27	theme	adiposity	1442:1450	arg1	following					1158:1166	the following	1154:1166	the following	1154:1166	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	4	28	theme	SRD-with-fish	852:864	arg1	%					878:878	SRD-with-fish oil [where 6%	852:878	SRD-with-fish oil [where 6% of the corn oil	852:894	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	4	28	theme	SRD-with-fish	852:864	arg1	oil					892:894	the corn oil	883:894	the corn oil	883:894	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	5	29	theme	period	1076:1081	arg1	end					1052:1054	the end	1048:1054	the end of the experimental period	1048:1081	Male offspring from RD-dams continued with RD up to the end of the experimental period, forming an RD-RD group.
26511757	0	30	theme	liver	89:93	arg1	steatosis					95:103	liver steatosis	89:103	liver steatosis	89:103	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	4	31	theme	[where	870:875	arg1	%					878:878	SRD-with-fish oil [where 6%	852:878	SRD-with-fish oil [where 6% of the corn oil	852:894	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	4	31	theme	[where	870:875	arg1	oil					892:894	the corn oil	883:894	the corn oil	883:894	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	6	32	theme	fatty	1506:1510	arg1	levels					1517:1522	plasma free fatty acid levels	1494:1522	plasma free fatty acid levels	1494:1522	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	2	33	theme	pregnancy	603:611	arg1	onset					594:598	the onset	590:598	the onset of pregnancy	590:611	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	1	34	theme	post-natal	268:277	arg1	diet					279:282	the post-natal diet	264:282	the post-natal diet	264:282	The interaction between fetal programming and the post-natal environment suggests that the post-natal diet could amplify or attenuate programmed outcomes.
26511757	6	35	theme	steatosis	1214:1222	arg1	content					1474:1480	limited body fat content	1457:1480	limited body fat content	1457:1480	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	35	theme	steatosis	1214:1222	arg1	normalization					1314:1326	the complete normalization	1301:1326	the complete normalization of CPT I activity and PPARα protein mass levels	1301:1374	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	35	theme	steatosis	1214:1222	arg1	prevention					1169:1178	prevention	1169:1178	prevention	1169:1178	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	35	theme	steatosis	1214:1222	arg1	accretion					1420:1428	a slight but not statistically significant accretion	1377:1428	a slight but not statistically significant accretion of visceral adiposity	1377:1450	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	35	theme	steatosis	1214:1222	arg1	following					1158:1166	the following	1154:1166	the following	1154:1166	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	36	theme	significant	1408:1418	arg1	accretion					1420:1428	a slight but not statistically significant accretion	1377:1428	a slight but not statistically significant accretion of visceral adiposity	1377:1450	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	37	theme	plasma	1494:1499	arg1	levels					1517:1522	plasma free fatty acid levels	1494:1522	plasma free fatty acid levels	1494:1522	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	9	38	theme	sucrose-rich	1820:1831	arg1	diet					1833:1836	a maternal and post-weaning sucrose-rich diet	1792:1836	a maternal and post-weaning sucrose-rich diet	1792:1836	These results suggest the capacity of n-3 PUFAs to overcome some adverse outcomes induced by a maternal and post-weaning sucrose-rich diet.
26511757	0	39	theme	post-suckling	11:23	arg1	acids					51:55	post-suckling n-3 polyunsaturated fatty acids	11:55	post-suckling n-3 polyunsaturated fatty acids	11:55	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	4	40	theme	oil	892:894	arg1	%					878:878	SRD-with-fish oil [where 6%	852:878	SRD-with-fish oil [where 6% of the corn oil	852:894	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	4	40	theme	oil	892:894	arg1	oil					892:894	the corn oil	883:894	the corn oil	883:894	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	6	41	theme	liver	1208:1212	arg1	steatosis					1214:1222	liver steatosis	1208:1222	liver steatosis	1208:1222	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	0	42	theme	polyunsaturated	29:43	arg1	acids					51:55	post-suckling n-3 polyunsaturated fatty acids	11:55	post-suckling n-3 polyunsaturated fatty acids	11:55	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	2	43	theme	dietary	356:362	arg1	acids					401:405	dietary n-3 long-chain polyunsaturated fatty acids	356:405	dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning	356:428	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	43	theme	dietary	356:362	arg1	PUFAs					412:416	n-3 PUFAs	408:416	n-3 PUFAs	408:416	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	6	44	theme	hypertriglyceridemia	1183:1202	arg1	content					1474:1480	limited body fat content	1457:1480	limited body fat content	1457:1480	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	44	theme	hypertriglyceridemia	1183:1202	arg1	normalization					1314:1326	the complete normalization	1301:1326	the complete normalization of CPT I activity and PPARα protein mass levels	1301:1374	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	44	theme	hypertriglyceridemia	1183:1202	arg1	prevention					1169:1178	prevention	1169:1178	prevention	1169:1178	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	44	theme	hypertriglyceridemia	1183:1202	arg1	accretion					1420:1428	a slight but not statistically significant accretion	1377:1428	a slight but not statistically significant accretion of visceral adiposity	1377:1450	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	44	theme	hypertriglyceridemia	1183:1202	arg1	following					1158:1166	the following	1154:1166	the following	1154:1166	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	45	from	presence	1112:1119	arg1	diet					1142:1145	the weaning diet	1130:1145	the weaning diet	1130:1145	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	2	46	from	onset	594:598	arg1	oil					580:582	corn oil	575:582	corn oil	575:582	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	46	from	onset	594:598	arg1	diet					540:543	a sucrose-rich diet	525:543	a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood	525:627	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	6	47	theme	maternal	1287:1294	arg1	SRD					1296:1298	a maternal SRD	1285:1298	a maternal SRD	1285:1298	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	7	48	theme	high-sucrose	1586:1597	arg1	diet					1599:1602	a high-sucrose diet	1584:1602	a high-sucrose diet challenge	1584:1612	All of these results were observed even in the presence of a high-sucrose diet challenge after weaning.
26511757	2	49	theme	fatty	395:399	arg1	acids					401:405	dietary n-3 long-chain polyunsaturated fatty acids	356:405	dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning	356:428	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	49	theme	fatty	395:399	arg1	PUFAs					412:416	n-3 PUFAs	408:416	n-3 PUFAs	408:416	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	1	50	theme	post-natal	227:236	arg1	environment					238:248	the post-natal environment	223:248	the post-natal environment	223:248	The interaction between fetal programming and the post-natal environment suggests that the post-natal diet could amplify or attenuate programmed outcomes.
26511757	0	51	dep	Effects	0:6	arg1	prevention					58:67	prevention	58:67	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.	0:175	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	2	52	theme	long-chain	368:377	arg1	acids					401:405	dietary n-3 long-chain polyunsaturated fatty acids	356:405	dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning	356:428	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	52	theme	long-chain	368:377	arg1	PUFAs					412:416	n-3 PUFAs	408:416	n-3 PUFAs	408:416	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	4	53	theme	5	936:936	arg1	%					937:937	%	937:937	%	937:937	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	6	54	theme	lipogenic	1249:1257	arg1	activity					1266:1273	increased lipogenic enzyme activity	1239:1273	increased lipogenic enzyme activity caused by a maternal SRD	1239:1298	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	7	55	dep	challenge	1604:1612	arg1	the					1568:1570	the	1568:1570	the	1568:1570	All of these results were observed even in the presence of a high-sucrose diet challenge after weaning.
26511757	7	55	dep	challenge	1604:1612	arg1	presence					1572:1579	presence	1572:1579	presence	1572:1579	All of these results were observed even in the presence of a high-sucrose diet challenge after weaning.
26511757	6	56	theme	I	1335:1335	arg1	activity					1337:1344	CPT I activity	1331:1344	CPT I activity	1331:1344	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	0	57	theme	acids	51:55	arg1	Effects					0:6	Effects	0:6	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.	0:175	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	8	58	theme	saturated	1665:1673	arg1	composition					1686:1696	a more saturated fatty acid composition	1658:1696	a more saturated fatty acid composition	1658:1696	SRD-dams' breast milk showed a more saturated fatty acid composition.
26511757	3	59	theme	rodent	708:713	arg1	diet					726:729	the standard powdered rodent commercial diet	686:729	the standard powdered rodent commercial diet (RD)	686:734	During pregnancy and lactation, dams were fed an SRD or the standard powdered rodent commercial diet (RD).
26511757	3	59	theme	rodent	708:713	arg1	RD					732:733	RD	732:733	RD	732:733	During pregnancy and lactation, dams were fed an SRD or the standard powdered rodent commercial diet (RD).
26511757	8	60	theme	acid	1681:1684	arg1	composition					1686:1696	a more saturated fatty acid composition	1658:1696	a more saturated fatty acid composition	1658:1696	SRD-dams' breast milk showed a more saturated fatty acid composition.
26511757	6	61	theme	complete	1305:1312	arg1	normalization					1314:1326	the complete normalization	1301:1326	the complete normalization of CPT I activity and PPARα protein mass levels	1301:1374	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	3	62	theme	standard	690:697	arg1	diet					726:729	the standard powdered rodent commercial diet	686:729	the standard powdered rodent commercial diet (RD)	686:734	During pregnancy and lactation, dams were fed an SRD or the standard powdered rodent commercial diet (RD).
26511757	3	62	theme	standard	690:697	arg1	RD					732:733	RD	732:733	RD	732:733	During pregnancy and lactation, dams were fed an SRD or the standard powdered rodent commercial diet (RD).
26511757	5	63	from	RD-dams	1016:1022	arg1	offspring					1001:1009	Male offspring	996:1009	Male offspring from RD-dams	996:1022	Male offspring from RD-dams continued with RD up to the end of the experimental period, forming an RD-RD group.
26511757	1	64	theme	fetal	201:205	arg1	programming					207:217	fetal programming	201:217	fetal programming	201:217	The interaction between fetal programming and the post-natal environment suggests that the post-natal diet could amplify or attenuate programmed outcomes.
26511757	6	65	theme	FO	1124:1125	arg1	presence					1112:1119	The presence	1108:1119	The presence of FO in the weaning diet	1108:1145	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	2	66	theme	sucrose-rich	527:538	arg1	oil					580:582	corn oil	575:582	corn oil	575:582	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	66	theme	sucrose-rich	527:538	arg1	diet					540:543	a sucrose-rich diet	525:543	a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood	525:627	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	67	dep	oil	580:582	arg1	SRD					546:548	SRD	546:548	SRD	546:548	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	68	theme	fat	561:563	arg1	oil					580:582	corn oil	575:582	corn oil	575:582	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	68	theme	fat	561:563	arg1	source					565:570	the fat source	557:570	the fat source	557:570	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	6	69	theme	body	1465:1468	arg1	content					1474:1480	limited body fat content	1457:1480	limited body fat content	1457:1480	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	9	70	theme	n-3	1737:1739	arg1	PUFAs					1741:1745	n-3 PUFAs	1737:1745	n-3 PUFAs	1737:1745	These results suggest the capacity of n-3 PUFAs to overcome some adverse outcomes induced by a maternal and post-weaning sucrose-rich diet.
26511757	6	71	theme	levels	1369:1374	arg1	content					1474:1480	limited body fat content	1457:1480	limited body fat content	1457:1480	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	71	theme	levels	1369:1374	arg1	normalization					1314:1326	the complete normalization	1301:1326	the complete normalization of CPT I activity and PPARα protein mass levels	1301:1374	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	71	theme	levels	1369:1374	arg1	prevention					1169:1178	prevention	1169:1178	prevention	1169:1178	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	71	theme	levels	1369:1374	arg1	accretion					1420:1428	a slight but not statistically significant accretion	1377:1428	a slight but not statistically significant accretion of visceral adiposity	1377:1450	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	71	theme	levels	1369:1374	arg1	following					1158:1166	the following	1154:1166	the following	1154:1166	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	72	theme	protein	1356:1362	arg1	levels					1369:1374	PPARα protein mass levels	1350:1374	PPARα protein mass levels	1350:1374	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	4	73	theme	%	937:937	arg1	FO					932:933	FO	932:933	FO	932:933	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	4	73	theme	%	937:937	arg1	oil					927:929	fish oil	922:929	fish oil (FO) 5%	922:937	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	4	74	from	SRD-dams	796:803	arg1	offspring					781:789	male offspring	776:789	male offspring from SRD-dams	776:803	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	2	75	theme	liver	489:493	arg1	amelioration					445:456	an amelioration	442:456	an amelioration of dyslipidemia	442:472	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	75	theme	liver	489:493	arg1	steatosis					495:503	liver steatosis	489:503	liver steatosis	489:503	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	5	76	theme	experimental	1063:1074	arg1	period					1076:1081	the experimental period	1059:1081	the experimental period	1059:1081	Male offspring from RD-dams continued with RD up to the end of the experimental period, forming an RD-RD group.
26511757	0	77	theme	steatosis	95:103	arg1	prevention					58:67	prevention	58:67	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.	0:175	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	6	78	theme	visceral	1433:1440	arg1	adiposity					1442:1450	visceral adiposity	1433:1450	visceral adiposity	1433:1450	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	79	theme	acid	1512:1515	arg1	levels					1517:1522	plasma free fatty acid levels	1494:1522	plasma free fatty acid levels	1494:1522	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	4	80	theme	oil	866:868	arg1	%					878:878	SRD-with-fish oil [where 6%	852:878	SRD-with-fish oil [where 6% of the corn oil	852:894	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	4	80	theme	oil	866:868	arg1	oil					892:894	the corn oil	883:894	the corn oil	883:894	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	6	81	theme	free	1501:1504	arg1	levels					1517:1522	plasma free fatty acid levels	1494:1522	plasma free fatty acid levels	1494:1522	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	9	82	theme	maternal	1794:1801	arg1	diet					1833:1836	a maternal and post-weaning sucrose-rich diet	1792:1836	a maternal and post-weaning sucrose-rich diet	1792:1836	These results suggest the capacity of n-3 PUFAs to overcome some adverse outcomes induced by a maternal and post-weaning sucrose-rich diet.
26511757	6	83	theme	increased	1239:1247	arg1	activity					1266:1273	increased lipogenic enzyme activity	1239:1273	increased lipogenic enzyme activity caused by a maternal SRD	1239:1298	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	0	84	theme	sucrose-rich	126:137	arg1	diet					139:142	a sucrose-rich diet	124:142	a sucrose-rich diet during pre- and post-natal life	124:174	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	9	85	theme	post-weaning	1807:1818	arg1	diet					1833:1836	a maternal and post-weaning sucrose-rich diet	1792:1836	a maternal and post-weaning sucrose-rich diet	1792:1836	These results suggest the capacity of n-3 PUFAs to overcome some adverse outcomes induced by a maternal and post-weaning sucrose-rich diet.
26511757	0	86	theme	n-3	25:27	arg1	acids					51:55	post-suckling n-3 polyunsaturated fatty acids	11:55	post-suckling n-3 polyunsaturated fatty acids	11:55	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	5	87	theme	Male	996:999	arg1	offspring					1001:1009	Male offspring	996:1009	Male offspring from RD-dams	996:1022	Male offspring from RD-dams continued with RD up to the end of the experimental period, forming an RD-RD group.
26511757	4	88	theme	fish	922:925	arg1	FO					932:933	FO	932:933	FO	932:933	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	4	88	theme	fish	922:925	arg1	oil					927:929	fish oil	922:929	fish oil (FO) 5%	922:937	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	0	89	theme	fatty	45:49	arg1	acids					51:55	post-suckling n-3 polyunsaturated fatty acids	11:55	post-suckling n-3 polyunsaturated fatty acids	11:55	Effects of post-suckling n-3 polyunsaturated fatty acids: prevention of dyslipidemia and liver steatosis induced in rats by a sucrose-rich diet during pre- and post-natal life.
26511757	4	90	theme	corn	887:890	arg1	oil					892:894	the corn oil	883:894	the corn oil	883:894	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	6	91	theme	weaning	1134:1140	arg1	diet					1142:1145	the weaning diet	1130:1145	the weaning diet	1130:1145	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	1	92	theme	programmed	311:320	arg1	outcomes					322:329	programmed outcomes	311:329	programmed outcomes	311:329	The interaction between fetal programming and the post-natal environment suggests that the post-natal diet could amplify or attenuate programmed outcomes.
26511757	2	93	theme	dyslipidemia	461:472	arg1	amelioration					445:456	an amelioration	442:456	an amelioration of dyslipidemia	442:472	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	93	theme	dyslipidemia	461:472	arg1	adiposity					475:483	adiposity	475:483	adiposity	475:483	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	93	theme	dyslipidemia	461:472	arg1	steatosis					495:503	liver steatosis	489:503	liver steatosis	489:503	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	6	94	attach	presence	1112:1119	arg2	FO					1124:1125	FO	1124:1125	FO	1124:1125	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	6	94	attach	presence	1112:1119	arg1	diet					1142:1145	the weaning diet	1130:1145	the weaning diet	1130:1145	The presence of FO in the weaning diet showed the following: prevention of hypertriglyceridemia and liver steatosis, together with increased lipogenic enzyme activity caused by a maternal SRD; the complete normalization of CPT I activity and PPARα protein mass levels; a slight but not statistically significant accretion of visceral adiposity; and limited body fat content and reduced plasma free fatty acid levels.
26511757	7	95	theme	diet	1599:1602	arg1	challenge					1604:1612	a high-sucrose diet challenge	1584:1612	a high-sucrose diet challenge	1584:1612	All of these results were observed even in the presence of a high-sucrose diet challenge after weaning.
26511757	4	96	theme	corn	943:946	arg1	CO					953:954	CO	953:954	CO	953:954	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	4	96	theme	corn	943:946	arg1	oil					948:950	corn oil	943:950	corn oil (CO) 1%]	943:959	At weaning and until 150 days of life, male offspring from SRD-dams were divided into two groups and fed an SRD or SRD-with-fish oil [where 6% of the corn oil was partially replaced by fish oil (FO) 5% and corn oil (CO) 1%], forming SRD-SRD or SRD-FO groups.
26511757	2	97	theme	polyunsaturated	379:393	arg1	acids					401:405	dietary n-3 long-chain polyunsaturated fatty acids	356:405	dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning	356:428	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
26511757	2	97	theme	polyunsaturated	379:393	arg1	PUFAs					412:416	n-3 PUFAs	408:416	n-3 PUFAs	408:416	We investigated whether dietary n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) at weaning resulted in an amelioration of dyslipidemia, adiposity and liver steatosis that was induced by a sucrose-rich diet (SRD; where the fat source is corn oil) from the onset of pregnancy up to adulthood.
24519987	8	0	theme	slices	1164:1169	arg1	volume					1148:1153	The specific volume	1135:1153	The specific volume of bread slices	1135:1169	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	7	1	theme	60	963:964	arg1	%					965:965	%	965:965	%	965:965	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	6	2	theme	pin-milling	923:933	arg1	speed					935:939	pin-milling speed	923:939	pin-milling speed	923:939	Water absorption increased significantly with increasing extraction rate and pin-milling speed.
24519987	8	3	theme	specific	1139:1146	arg1	volume					1148:1153	The specific volume	1135:1153	The specific volume of bread slices	1135:1169	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	5	4	theme	extraction	804:813	arg1	rate					815:818	extraction rate	804:818	extraction rate	804:818	Flour particle size and starch damage were significantly impacted by extraction rate and speed of pin-milling.
24519987	8	5	theme	commercial	1378:1387	arg1	flour					1389:1393	commercial flour	1378:1393	commercial flour	1378:1393	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	5	theme	commercial	1378:1387	arg1	pin-milling					1399:1409	no pin-milling	1396:1409	no pin-milling	1396:1409	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	5	6	theme	pin-milling	833:843	arg1	speed					824:828	speed	824:828	speed of pin-milling	824:843	Flour particle size and starch damage were significantly impacted by extraction rate and speed of pin-milling.
24519987	5	6	theme	pin-milling	833:843	arg1	rate					815:818	extraction rate	804:818	extraction rate	804:818	Flour particle size and starch damage were significantly impacted by extraction rate and speed of pin-milling.
24519987	0	7	theme	bread	91:95	arg1	properties					65:74	quality properties	57:74	quality properties of gluten-free bread	57:95	Effect of sorghum flour composition and particle size on quality properties of gluten-free bread.
24519987	0	8	from	Effect	0:5	arg1	properties					65:74	quality properties	57:74	quality properties of gluten-free bread	57:95	Effect of sorghum flour composition and particle size on quality properties of gluten-free bread.
24519987	7	9	theme	crumb	1034:1038	arg1	properties					1040:1049	better crumb properties	1027:1049	better crumb properties	1027:1049	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	7	10	theme	lower	1056:1060	arg1	firmness					1068:1075	lower crumb firmness	1056:1075	lower crumb firmness	1056:1075	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	4	11	theme	extraction	668:677	arg1	rate					679:682	varying extraction rate	660:682	varying extraction rate	660:682	Significant differences were found (P < 0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents.
24519987	4	12	theme	flours	650:655	arg1	composition					627:637	the composition	623:637	the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents	623:732	Significant differences were found (P < 0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents.
24519987	0	13	theme	gluten-free	79:89	arg1	bread					91:95	gluten-free bread	79:95	gluten-free bread	79:95	Effect of sorghum flour composition and particle size on quality properties of gluten-free bread.
24519987	8	14	theme	%	1243:1243	arg1	extraction					1245:1254	60% extraction	1241:1254	60% extraction	1241:1254	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	4	15	theme	varying	660:666	arg1	rate					679:682	varying extraction rate	660:682	varying extraction rate	660:682	Significant differences were found (P < 0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents.
24519987	6	16	theme	extraction	903:912	arg1	rate					914:917	extraction rate	903:917	extraction rate	903:917	Water absorption increased significantly with increasing extraction rate and pin-milling speed.
24519987	4	17	located	found	603:607	arg2	differences					586:596	Significant differences	574:596	Significant differences	574:596	Significant differences were found (P < 0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents.
24519987	4	17	located	found	603:607	arg1	composition					627:637	the composition	623:637	the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents	623:732	Significant differences were found (P < 0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents.
24519987	1	18	from	speeds	221:226	arg1	pin-milled					197:206	pin-milled	197:206	pin-milled	197:206	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	4	19	dep	found	603:607	arg1	P < 0.05					610:617	P < 0.05	610:617	P < 0.05	610:617	Significant differences were found (P < 0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents.
24519987	8	20	theme	bread	1158:1162	arg1	slices					1164:1169	bread slices	1158:1169	bread slices	1158:1169	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	7	21	theme	crumb	1062:1066	arg1	firmness					1068:1075	lower crumb firmness	1056:1075	lower crumb firmness	1056:1075	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	7	22	theme	better	1027:1032	arg1	properties					1040:1049	better crumb properties	1027:1049	better crumb properties	1027:1049	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	7	23	theme	flour	1122:1126	arg1	types					1128:1132	all other extractions and flour types	1096:1132	types	1128:1132	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	4	24	theme	rate	679:682	arg1	flours					650:655	sorghum flours	642:655	sorghum flours	642:655	Significant differences were found (P < 0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents.
24519987	1	25	theme	extraction	155:164	arg1	rates					166:170	extraction rates	155:170	extraction rates (60%, 80%, and 100%)	155:191	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	2	26	theme	flour	367:371	arg1	composition					373:383	flour composition	367:383	flour composition	367:383	Flours were characterized for flour composition, total starch content, particle size distribution, color, damaged starch, and water absorption.
24519987	7	27	theme	specific	1009:1016	arg1	volumes					1018:1024	significantly higher specific volumes	988:1024	significantly higher specific volumes	988:1024	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	2	28	theme	size	417:420	arg1	distribution					422:433	particle size distribution	408:433	particle size distribution	408:433	Flours were characterized for flour composition, total starch content, particle size distribution, color, damaged starch, and water absorption.
24519987	4	29	theme	sorghum	642:648	arg1	flours					650:655	sorghum flours	642:655	sorghum flours	642:655	Significant differences were found (P < 0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents.
24519987	8	30	dep	1096.26 g	1367:1375	arg1	flour					1389:1393	commercial flour	1378:1393	commercial flour	1378:1393	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	30	dep	1096.26 g	1367:1375	arg1	pin-milling					1399:1409	no pin-milling	1396:1409	no pin-milling	1396:1409	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	7	31	theme	other	1100:1104	arg1	extractions					1106:1116	all other extractions and flour types	1096:1132	extractions	1106:1116	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	8	32	dep	2.01 mL/g	1183:1191	arg1	extraction					1199:1208	100% extraction	1194:1208	100% extraction	1194:1208	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	32	dep	2.01 mL/g	1183:1191	arg1	pin-milling					1214:1224	no pin-milling	1211:1224	no pin-milling	1211:1224	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	7	33	contain	had	984:986	arg2	properties					1040:1049	better crumb properties	1027:1049	better crumb properties	1027:1049	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	7	33	contain	had	984:986	arg1	Breads					942:947	Breads	942:947	Breads produced from 60% extraction flour	942:982	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	7	33	contain	had	984:986	arg2	volumes					1018:1024	significantly higher specific volumes	988:1024	significantly higher specific volumes	988:1024	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	7	33	contain	had	984:986	arg2	firmness					1068:1075	lower crumb firmness	1056:1075	lower crumb firmness	1056:1075	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	3	34	theme	crumb	558:562	arg1	firmness					564:571	crumb firmness	558:571	crumb firmness	558:571	Bread was characterized for specific volume, crumb structure properties, and crumb firmness.
24519987	0	35	theme	flour	18:22	arg1	composition					24:34	sorghum flour composition	10:34	sorghum flour composition	10:34	Effect of sorghum flour composition and particle size on quality properties of gluten-free bread.
24519987	1	36	theme	different	211:219	arg1	speeds					221:226	different speeds	211:226	different speeds (no pin-milling, low-speed, and high-speed)	211:270	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	4	37	theme	total	712:716	arg1	contents					725:732	total starch contents	712:732	total starch contents	712:732	Significant differences were found (P < 0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents.
24519987	8	38	dep	extraction	1245:1254	arg1	pin-milling					1267:1277	pin-milling	1267:1277	pin-milling	1267:1277	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	0	39	theme	sorghum	10:16	arg1	composition					24:34	sorghum flour composition	10:34	sorghum flour composition	10:34	Effect of sorghum flour composition and particle size on quality properties of gluten-free bread.
24519987	7	40	theme	higher	1002:1007	arg1	volumes					1018:1024	significantly higher specific volumes	988:1024	significantly higher specific volumes	988:1024	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	8	41	theme	%	1326:1326	arg1	extraction					1328:1337	60% extraction	1324:1337	60% extraction	1324:1337	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	41	theme	%	1326:1326	arg1	pin-milling					1351:1361	high-speed pin-milling	1340:1361	high-speed pin-milling	1340:1361	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	1	42	theme	White	98:102	arg1	sorghum					116:122	White, food-grade sorghum	98:122	White, food-grade sorghum	98:122	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	5	43	theme	Flour	735:739	arg1	size					750:753	Flour particle size	735:753	Flour particle size	735:753	Flour particle size and starch damage were significantly impacted by extraction rate and speed of pin-milling.
24519987	2	44	theme	starch	392:397	arg1	content					399:405	total starch content	386:405	total starch content	386:405	Flours were characterized for flour composition, total starch content, particle size distribution, color, damaged starch, and water absorption.
24519987	8	45	dep	553.28 g	1314:1321	arg1	extraction					1328:1337	60% extraction	1324:1337	60% extraction	1324:1337	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	45	dep	553.28 g	1314:1321	arg1	pin-milling					1351:1361	high-speed pin-milling	1340:1361	high-speed pin-milling	1340:1361	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	45	dep	553.28 g	1314:1321	arg1	1096.26 g					1367:1375	1096.26 g	1367:1375	1096.26 g	1367:1375	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	45	dep	553.28 g	1314:1321	arg1	to					1364:1365	to	1364:1365	to	1364:1365	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	1	46	theme	variable	297:304	arg1	composition					306:316	variable composition	297:316	variable composition	297:316	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	1	47	dep	rates	166:170	arg1	%					175:175	60%	173:175	60%	173:175	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	1	47	dep	rates	166:170	arg1	%					180:180	80%	178:180	80%	178:180	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	1	47	dep	rates	166:170	arg1	%					190:190	100%	187:190	100%	187:190	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	4	48	theme	Significant	574:584	arg1	differences					586:596	Significant differences	574:596	Significant differences	574:596	Significant differences were found (P < 0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents.
24519987	5	49	theme	particle	741:748	arg1	size					750:753	Flour particle size	735:753	Flour particle size	735:753	Flour particle size and starch damage were significantly impacted by extraction rate and speed of pin-milling.
24519987	0	50	theme	composition	24:34	arg1	Effect					0:5	Effect	0:5	Effect of sorghum flour composition and particle size on quality properties of gluten-free bread.	0:96	Effect of sorghum flour composition and particle size on quality properties of gluten-free bread.
24519987	2	51	theme	total	386:390	arg1	content					399:405	total starch content	386:405	total starch content	386:405	Flours were characterized for flour composition, total starch content, particle size distribution, color, damaged starch, and water absorption.
24519987	1	52	theme	composition	306:316	arg1	flours					282:287	flours	282:287	flours of both variable composition and particle size	282:334	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	3	53	theme	specific	509:516	arg1	volume					518:523	specific volume	509:523	specific volume	509:523	Bread was characterized for specific volume, crumb structure properties, and crumb firmness.
24519987	2	54	theme	damaged	443:449	arg1	starch					451:456	damaged starch	443:456	damaged starch	443:456	Flours were characterized for flour composition, total starch content, particle size distribution, color, damaged starch, and water absorption.
24519987	0	55	theme	size	49:52	arg1	Effect					0:5	Effect	0:5	Effect of sorghum flour composition and particle size on quality properties of gluten-free bread.	0:96	Effect of sorghum flour composition and particle size on quality properties of gluten-free bread.
24519987	6	56	theme	Water	846:850	arg1	absorption					852:861	Water absorption	846:861	Water absorption	846:861	Water absorption increased significantly with increasing extraction rate and pin-milling speed.
24519987	7	57	theme	%	965:965	arg1	flour					978:982	60% extraction flour	963:982	60% extraction flour	963:982	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	8	58	dep	ranged	1171:1176	arg1	extraction					1245:1254	60% extraction	1241:1254	60% extraction	1241:1254	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	58	dep	ranged	1171:1176	arg1	whereas					1281:1287	whereas	1281:1287	whereas	1281:1287	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	0	59	theme	particle	40:47	arg1	size					49:52	particle size	40:52	particle size	40:52	Effect of sorghum flour composition and particle size on quality properties of gluten-free bread.
24519987	7	60	theme	extraction	967:976	arg1	flour					978:982	60% extraction flour	963:982	60% extraction flour	963:982	Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types.
24519987	1	61	theme	particle	322:329	arg1	size					331:334	particle size	322:334	particle size	322:334	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	4	62	theme	starch	718:723	arg1	contents					725:732	total starch contents	712:732	total starch contents	712:732	Significant differences were found (P < 0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents.
24519987	9	63	theme	fiber	1535:1539	arg1	P < 0.05					1550:1557	P < 0.05	1550:1557	P < 0.05	1550:1557	The bread characteristics were significantly impacted by flour properties, specifically particle size, starch damage, and fiber content (P < 0.05).
24519987	9	63	theme	fiber	1535:1539	arg1	content					1541:1547	fiber content	1535:1547	fiber content (P < 0.05)	1535:1558	The bread characteristics were significantly impacted by flour properties, specifically particle size, starch damage, and fiber content (P < 0.05).
24519987	8	64	theme	%	1197:1197	arg1	extraction					1199:1208	100% extraction	1194:1208	100% extraction	1194:1208	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	64	theme	%	1197:1197	arg1	pin-milling					1214:1224	no pin-milling	1211:1224	no pin-milling	1211:1224	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	65	theme	60	1324:1325	arg1	%					1326:1326	%	1326:1326	%	1326:1326	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	66	theme	100	1194:1196	arg1	%					1197:1197	%	1197:1197	%	1197:1197	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	1	67	theme	size	331:334	arg1	flours					282:287	flours	282:287	flours of both variable composition and particle size	282:334	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	3	68	theme	crumb	526:530	arg1	properties					542:551	crumb structure properties	526:551	crumb structure properties	526:551	Bread was characterized for specific volume, crumb structure properties, and crumb firmness.
24519987	2	69	theme	water	463:467	arg1	absorption					469:478	water absorption	463:478	water absorption	463:478	Flours were characterized for flour composition, total starch content, particle size distribution, color, damaged starch, and water absorption.
24519987	3	70	theme	structure	532:540	arg1	properties					542:551	crumb structure properties	526:551	crumb structure properties	526:551	Bread was characterized for specific volume, crumb structure properties, and crumb firmness.
24519987	8	71	theme	high-speed	1340:1349	arg1	extraction					1328:1337	60% extraction	1324:1337	60% extraction	1324:1337	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	8	71	theme	high-speed	1340:1349	arg1	pin-milling					1351:1361	high-speed pin-milling	1340:1361	high-speed pin-milling	1340:1361	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	0	72	theme	quality	57:63	arg1	properties					65:74	quality properties	57:74	quality properties of gluten-free bread	57:95	Effect of sorghum flour composition and particle size on quality properties of gluten-free bread.
24519987	8	73	theme	60	1241:1242	arg1	%					1243:1243	%	1243:1243	%	1243:1243	The specific volume of bread slices ranged from 2.01 mL/g (100% extraction, no pin-milling) to 2.54 mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28 g (60% extraction, high-speed pin-milling) to 1096.26 g (commercial flour, no pin-milling).
24519987	9	74	theme	particle	1501:1508	arg1	size					1510:1513	particle size	1501:1513	particle size	1501:1513	The bread characteristics were significantly impacted by flour properties, specifically particle size, starch damage, and fiber content (P < 0.05).
24519987	5	75	theme	starch	759:764	arg1	damage					766:771	starch damage	759:771	starch damage	759:771	Flour particle size and starch damage were significantly impacted by extraction rate and speed of pin-milling.
24519987	9	76	theme	flour	1470:1474	arg1	properties					1476:1485	flour properties	1470:1485	flour properties	1470:1485	The bread characteristics were significantly impacted by flour properties, specifically particle size, starch damage, and fiber content (P < 0.05).
24519987	2	77	theme	particle	408:415	arg1	distribution					422:433	particle size distribution	408:433	particle size distribution	408:433	Flours were characterized for flour composition, total starch content, particle size distribution, color, damaged starch, and water absorption.
24519987	1	78	dep	speeds	221:226	arg1	high-speed					260:269	high-speed	260:269	high-speed	260:269	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	1	78	dep	speeds	221:226	arg1	pin-milling					232:242	pin-milling	232:242	pin-milling	232:242	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	1	78	dep	speeds	221:226	arg1	low-speed					245:253	low-speed	245:253	low-speed	245:253	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
24519987	9	79	theme	starch	1516:1521	arg1	damage					1523:1528	starch damage	1516:1528	starch damage	1516:1528	The bread characteristics were significantly impacted by flour properties, specifically particle size, starch damage, and fiber content (P < 0.05).
24519987	9	80	theme	bread	1417:1421	arg1	characteristics					1423:1437	The bread characteristics	1413:1437	The bread characteristics	1413:1437	The bread characteristics were significantly impacted by flour properties, specifically particle size, starch damage, and fiber content (P < 0.05).
24519987	1	81	dep	White	98:102	arg1	food-grade					105:114	food-grade	105:114	food-grade	105:114	White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size.
25600967	0	0	theme	nanoparticles	65:77	arg1	targeting					52:60	the specific targeting	39:60	the specific targeting of nanoparticles	39:77	Crucial role of the protein corona for the specific targeting of nanoparticles.
25600967	5	1	theme	barrier	783:789	arg1	cells					791:795	the brain endothelial barrier cells	761:795	the brain endothelial barrier cells	761:795	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	1	2	theme	superparamagnetic	140:156	arg1	SPIONs					184:189	SPIONs	184:189	SPIONs	184:189	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	1	2	theme	superparamagnetic	140:156	arg1	nanoparticles					169:181	superparamagnetic iron oxide nanoparticles	140:181	superparamagnetic iron oxide nanoparticles (SPIONs)	140:190	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	6	3	dep	tissue	941:946	arg1	e.g.					929:932	e.g.	929:932	e.g.	929:932	CONCLUSION Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
25600967	1	4	dep	AIMS	80:83	arg1	aimed					88:92	aimed	88:92	aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues	88:277	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	6	5	theme	brain	935:939	arg1	tissue					941:946	brain tissue	935:946	brain tissue	935:946	CONCLUSION Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
25600967	5	6	theme	mouse	656:660	arg1	model					682:686	the mouse blood-brain barrier model	652:686	the mouse blood-brain barrier model	652:686	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	2	7	theme	&	290:290	arg1	SPIONs					300:305	MATERIALS & METHODS SPIONs	280:305	MATERIALS & METHODS SPIONs of different sizes and surface charges	280:344	MATERIALS & METHODS SPIONs of different sizes and surface charges were exposed to fetal bovine serum.
25600967	2	8	theme	METHODS	292:298	arg1	SPIONs					300:305	MATERIALS & METHODS SPIONs	280:305	MATERIALS & METHODS SPIONs of different sizes and surface charges	280:344	MATERIALS & METHODS SPIONs of different sizes and surface charges were exposed to fetal bovine serum.
25600967	4	9	theme	surface	599:605	arg1	charge					607:612	surface charge	599:612	surface charge	599:612	RESULTS & DISCUSSION The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs.
25600967	5	10	theme	blood-brain	662:672	arg1	model					682:686	the mouse blood-brain barrier model	652:686	the mouse blood-brain barrier model	652:686	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	1	11	from	effects	129:135	arg1	issues					272:277	their correspondence toxicological issues	237:277	their correspondence toxicological issues	237:277	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	1	11	from	effects	129:135	arg1	composition					199:209	the composition	195:209	the composition of the protein corona	195:231	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	2	12	theme	MATERIALS	280:288	arg1	SPIONs					300:305	MATERIALS & METHODS SPIONs	280:305	MATERIALS & METHODS SPIONs of different sizes and surface charges	280:344	MATERIALS & METHODS SPIONs of different sizes and surface charges were exposed to fetal bovine serum.
25600967	6	13	dep	CONCLUSION	798:807	arg1	have					893:896	have	893:896	may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity	889:973	CONCLUSION Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
25600967	1	14	theme	physicochemical	113:127	arg1	effects					129:135	the physicochemical effects	109:135	the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues	109:277	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	6	15	theme	available	827:835	arg1	nanoparticles					837:849	Some commercially available nanoparticles	809:849	Some commercially available nanoparticles used for target-specific applications	809:887	CONCLUSION Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
25600967	1	16	theme	oxide	163:167	arg1	SPIONs					184:189	SPIONs	184:189	SPIONs	184:189	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	1	16	theme	oxide	163:167	arg1	nanoparticles					169:181	superparamagnetic iron oxide nanoparticles	140:181	superparamagnetic iron oxide nanoparticles (SPIONs)	140:190	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	1	17	theme	toxicological	258:270	arg1	issues					272:277	their correspondence toxicological issues	237:277	their correspondence toxicological issues	237:277	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	3	18	theme	biological	412:421	arg1	effects					423:429	The structure/composition and biological effects	382:429	The structure/composition and biological effects of the protein corona-SPION complexes	382:467	The structure/composition and biological effects of the protein corona-SPION complexes were probed.
25600967	6	19	theme	unintended	898:907	arg1	uptake					909:914	unintended uptake	898:914	unintended uptake in the body	898:926	CONCLUSION Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
25600967	1	20	theme	nanoparticles	169:181	arg1	effects					129:135	the physicochemical effects	109:135	the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues	109:277	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	6	21	from	uptake	909:914	arg1	body					923:926	the body	919:926	the body	919:926	CONCLUSION Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
25600967	2	22	theme	different	310:318	arg1	sizes					320:324	different sizes	310:324	different sizes	310:324	MATERIALS & METHODS SPIONs of different sizes and surface charges were exposed to fetal bovine serum.
25600967	0	23	theme	Crucial	0:6	arg1	role					8:11	Crucial role	0:11	Crucial role of the protein corona for the specific targeting of nanoparticles	0:77	Crucial role of the protein corona for the specific targeting of nanoparticles.
25600967	6	24	theme	potential	954:962	arg1	cytotoxity					964:973	potential cytotoxity	954:973	potential cytotoxity	954:973	CONCLUSION Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
25600967	2	25	theme	bovine	368:373	arg1	serum					375:379	fetal bovine serum	362:379	fetal bovine serum	362:379	MATERIALS & METHODS SPIONs of different sizes and surface charges were exposed to fetal bovine serum.
25600967	4	26	dep	DISCUSSION	492:501	arg1	dependent					573:581	dependent	573:581	dependent	573:581	RESULTS & DISCUSSION The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs.
25600967	5	27	theme	nontargeted	702:712	arg1	SPIONs					714:719	nontargeted SPIONs	702:719	nontargeted SPIONs containing specific proteins	702:748	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	6	28	contain	have	893:896	arg1	nanoparticles					837:849	Some commercially available nanoparticles	809:849	Some commercially available nanoparticles used for target-specific applications	809:887	CONCLUSION Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
25600967	6	28	contain	have	893:896	arg2	uptake					909:914	unintended uptake	898:914	unintended uptake in the body	898:926	CONCLUSION Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
25600967	5	29	contain	containing	721:730	arg1	SPIONs					714:719	nontargeted SPIONs	702:719	nontargeted SPIONs containing specific proteins	702:748	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	5	29	contain	containing	721:730	arg2	proteins					741:748	specific proteins	732:748	specific proteins	732:748	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	6	30	dep	have	893:896	arg1	tissue					941:946	brain tissue	935:946	brain tissue	935:946	CONCLUSION Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
25600967	1	31	theme	iron	158:161	arg1	SPIONs					184:189	SPIONs	184:189	SPIONs	184:189	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	1	31	theme	iron	158:161	arg1	nanoparticles					169:181	superparamagnetic iron oxide nanoparticles	140:181	superparamagnetic iron oxide nanoparticles (SPIONs)	140:190	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	0	32	theme	corona	28:33	arg1	role					8:11	Crucial role	0:11	Crucial role of the protein corona for the specific targeting of nanoparticles	0:77	Crucial role of the protein corona for the specific targeting of nanoparticles.
25600967	3	33	theme	complexes	459:467	arg1	effects					423:429	The structure/composition and biological effects	382:429	The structure/composition and biological effects of the protein corona-SPION complexes	382:467	The structure/composition and biological effects of the protein corona-SPION complexes were probed.
25600967	0	34	theme	protein	20:26	arg1	corona					28:33	the protein corona	16:33	the protein corona	16:33	Crucial role of the protein corona for the specific targeting of nanoparticles.
25600967	5	35	theme	specific	732:739	arg1	proteins					741:748	specific proteins	732:748	specific proteins	732:748	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	4	36	dep	size	590:593	arg1	the					586:588	the	586:588	the	586:588	RESULTS & DISCUSSION The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs.
25600967	4	37	theme	SPIONs	621:626	arg1	size					590:593	size	590:593	size	590:593	RESULTS & DISCUSSION The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs.
25600967	4	37	theme	SPIONs	621:626	arg1	charge					607:612	surface charge	599:612	surface charge	599:612	RESULTS & DISCUSSION The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs.
25600967	4	38	dep	affinity	507:514	arg1	The					503:505	The	503:505	The	503:505	RESULTS & DISCUSSION The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs.
25600967	3	39	theme	structure/composition	386:406	arg1	effects					423:429	The structure/composition and biological effects	382:429	The structure/composition and biological effects of the protein corona-SPION complexes	382:467	The structure/composition and biological effects of the protein corona-SPION complexes were probed.
25600967	4	40	theme	adsorption	529:538	arg1	level					520:524	level	520:524	level	520:524	RESULTS & DISCUSSION The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs.
25600967	4	40	theme	adsorption	529:538	arg1	affinity					507:514	affinity	507:514	affinity	507:514	RESULTS & DISCUSSION The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs.
25600967	5	41	theme	barrier	674:680	arg1	model					682:686	the mouse blood-brain barrier model	652:686	the mouse blood-brain barrier model	652:686	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	3	42	theme	protein	438:444	arg1	complexes					459:467	the protein corona-SPION complexes	434:467	the protein corona-SPION complexes	434:467	The structure/composition and biological effects of the protein corona-SPION complexes were probed.
25600967	2	43	theme	charges	338:344	arg1	SPIONs					300:305	MATERIALS & METHODS SPIONs	280:305	MATERIALS & METHODS SPIONs of different sizes and surface charges	280:344	MATERIALS & METHODS SPIONs of different sizes and surface charges were exposed to fetal bovine serum.
25600967	5	44	dep	In	629:630	arg1	vivo					632:635	vivo	632:635	vivo	632:635	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	4	45	theme	proteins	552:559	arg1	adsorption					529:538	adsorption	529:538	adsorption of specific proteins	529:559	RESULTS & DISCUSSION The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs.
25600967	5	46	theme	In	629:630	arg1	experiments					637:647	In vivo experiments	629:647	In vivo experiments on the mouse blood-brain barrier model	629:686	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	2	47	theme	surface	330:336	arg1	charges					338:344	surface charges	330:344	surface charges	330:344	MATERIALS & METHODS SPIONs of different sizes and surface charges were exposed to fetal bovine serum.
25600967	4	48	theme	specific	543:550	arg1	proteins					552:559	specific proteins	543:559	specific proteins	543:559	RESULTS & DISCUSSION The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs.
25600967	5	49	theme	endothelial	771:781	arg1	cells					791:795	the brain endothelial barrier cells	761:795	the brain endothelial barrier cells	761:795	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	5	50	from	experiments	637:647	arg1	model					682:686	the mouse blood-brain barrier model	652:686	the mouse blood-brain barrier model	652:686	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	2	51	theme	sizes	320:324	arg1	SPIONs					300:305	MATERIALS & METHODS SPIONs	280:305	MATERIALS & METHODS SPIONs of different sizes and surface charges	280:344	MATERIALS & METHODS SPIONs of different sizes and surface charges were exposed to fetal bovine serum.
25600967	5	52	theme	brain	765:769	arg1	cells					791:795	the brain endothelial barrier cells	761:795	the brain endothelial barrier cells	761:795	In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
25600967	6	53	theme	target-specific	860:874	arg1	applications					876:887	target-specific applications	860:887	target-specific applications	860:887	CONCLUSION Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
25600967	2	54	theme	fetal	362:366	arg1	serum					375:379	fetal bovine serum	362:379	fetal bovine serum	362:379	MATERIALS & METHODS SPIONs of different sizes and surface charges were exposed to fetal bovine serum.
25600967	3	55	theme	corona-SPION	446:457	arg1	complexes					459:467	the protein corona-SPION complexes	434:467	the protein corona-SPION complexes	434:467	The structure/composition and biological effects of the protein corona-SPION complexes were probed.
25600967	1	56	theme	protein	218:224	arg1	corona					226:231	the protein corona	214:231	the protein corona	214:231	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	0	57	theme	specific	43:50	arg1	targeting					52:60	the specific targeting	39:60	the specific targeting of nanoparticles	39:77	Crucial role of the protein corona for the specific targeting of nanoparticles.
25600967	1	58	theme	corona	226:231	arg1	issues					272:277	their correspondence toxicological issues	237:277	their correspondence toxicological issues	237:277	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25600967	1	58	theme	corona	226:231	arg1	composition					199:209	the composition	195:209	the composition of the protein corona	195:231	AIMS We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
25162640	8	0	theme	%	1472:1472	arg1	3.0					1485:1487	3.0	1485:1487	3.0	1485:1487	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	8	0	theme	%	1472:1472	arg1	CI					1474:1475	95% CI	1470:1475	95% CI	1470:1475	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	8	0	theme	%	1472:1472	arg1	P					1490:1490	P	1490:1490	P	1490:1490	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	1	1	theme	chondroitin	240:250	arg1	sulfate					252:258	oversulfated chondroitin sulfate	227:258	oversulfated chondroitin sulfate	227:258	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	5	2	dep	outcome	1036:1042	arg1	stroke					1068:1073	stroke	1068:1073	stroke	1068:1073	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	5	2	dep	outcome	1036:1042	arg1	infarction					1056:1065	myocardial infarction	1045:1065	myocardial infarction	1045:1065	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	5	2	dep	outcome	1036:1042	arg1	dialysis					1087:1094	dialysis	1087:1094	dialysis	1087:1094	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	5	2	dep	outcome	1036:1042	arg1	arrest					1105:1110	cardiac arrest	1097:1110	cardiac arrest	1097:1110	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	5	2	dep	outcome	1036:1042	arg1	pneumonia					1076:1084	pneumonia	1076:1084	pneumonia	1076:1084	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	3	3	theme	center	539:544	arg1	study					588:592	a single center, retrospective, propensity-matched cohort study	530:592	study	588:592	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	3	4	from	frame	642:646	arg1	years					692:696	the two following years	674:696	the two following years	674:696	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	3	4	from	frame	642:646	arg1	preceding					661:669	preceding	661:669	preceding	661:669	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	1	5	theme	patients	168:175	arg1	millions					156:163	millions	156:163	millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions	156:309	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	3	6	theme	propensity-matched	562:579	arg1	study					588:592	a single center, retrospective, propensity-matched cohort study	530:592	study	588:592	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	9	7	dep	%	1794:1794	arg1	P = 0.04					1812:1819	P = 0.04	1812:1819	P = 0.04	1812:1819	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	7	dep	%	1794:1794	arg1	1.4					1807:1809	1.4	1807:1809	1.4	1807:1809	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	8	theme	median	1647:1652	arg1	ICU					1654:1656	the median ICU	1643:1656	the median ICU	1643:1656	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	3	9	theme	retrospective	547:559	arg1	study					588:592	a single center, retrospective, propensity-matched cohort study	530:592	study	588:592	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	9	10	dep	hours	1682:1686	arg1	P = 0.0004					1734:1743	P = 0.0004	1734:1743	P = 0.0004	1734:1743	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	10	dep	hours	1682:1686	arg1	S = 3299					1724:1731	S = 3299	1724:1731	S = 3299	1724:1731	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	10	dep	hours	1682:1686	arg1	range					1703:1707	interquartile range -26.6 to 79.8	1689:1721	interquartile range -26.6 to 79.8	1689:1721	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	1	11	theme	received	187:194	arg1	heparin					196:202	the US received heparin	180:202	the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions	180:309	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	1	11	theme	received	187:194	arg1	CH					218:219	CH	218:219	CH	218:219	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	11	12	theme	single	1923:1928	arg1	study					1937:1941	this single center study	1918:1941	this single center study	1918:1941	CONCLUSIONS The results from this single center study suggest the possibility that contaminated heparin might have contributed to serious morbidity following cardiac surgery.
25162640	8	13	theme	=	1395:1395	arg1	%					1422:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	6	14	theme	ICU	1199:1201	arg1	length					1203:1208	ICU length	1199:1208	ICU length of stay	1199:1216	Cox regression was used to determine the association between CH and ICU length of stay.
25162640	11	15	theme	serious	2019:2025	arg1	morbidity					2027:2035	serious morbidity	2019:2035	serious morbidity	2019:2035	CONCLUSIONS The results from this single center study suggest the possibility that contaminated heparin might have contributed to serious morbidity following cardiac surgery.
25162640	4	16	theme	independent	893:903	arg1	investigators					905:917	independent investigators	893:917	independent investigators	893:917	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	8	17	theme	%	1400:1400	arg1	%					1422:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	5	18	theme	myocardial	1045:1054	arg1	stroke					1068:1073	stroke	1068:1073	stroke	1068:1073	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	5	18	theme	myocardial	1045:1054	arg1	infarction					1056:1065	myocardial infarction	1045:1065	myocardial infarction	1045:1065	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	5	18	theme	myocardial	1045:1054	arg1	dialysis					1087:1094	dialysis	1087:1094	dialysis	1087:1094	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	5	18	theme	myocardial	1045:1054	arg1	arrest					1105:1110	cardiac arrest	1097:1110	cardiac arrest	1097:1110	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	5	18	theme	myocardial	1045:1054	arg1	pneumonia					1076:1084	pneumonia	1076:1084	pneumonia	1076:1084	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	8	19	dep	%	1368:1368	arg1	%					1422:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	8	19	dep	%	1368:1368	arg1	P					1425:1425	P < 0.001	1425:1433	P < 0.001	1425:1433	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	9	20	theme	non-significant	1535:1549	arg1	increase					1551:1558	a non-significant increase	1533:1558	a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1)	1533:1640	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	8	21	dep	3.0	1485:1487	arg1	to					1482:1483	to	1482:1483	to	1482:1483	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	1	22	dep	BACKGROUND	106:115	arg1	likely					144:149	likely	144:149	likely	144:149	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	2	23	theme	cardiac	479:485	arg1	surgery					487:493	adult cardiac surgery	473:493	adult cardiac surgery	473:493	We tested the hypothesis that CH was associated with serious morbidity, mortality, intensive care unit (ICU) stay and heparin-induced thrombocytopenia following adult cardiac surgery.
25162640	8	24	theme	CI	1407:1408	arg1	%					1422:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	0	25	theme	propensity-matched	73:90	arg1	study					99:103	a retrospective propensity-matched cohort study	57:103	a retrospective propensity-matched cohort study	57:103	Contaminated heparin and outcomes after cardiac surgery: a retrospective propensity-matched cohort study.
25162640	8	26	theme	%	1413:1413	arg1	%					1422:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	10	27	theme	nadir	1850:1854	arg1	counts					1865:1870	nadir platelet counts	1850:1870	nadir platelet counts	1850:1870	There was no difference in nadir platelet counts between cohorts.
25162640	8	28	theme	more	1319:1322	arg1	outcomes					1332:1339	more adverse outcomes	1319:1339	more adverse outcomes	1319:1339	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	0	29	dep	study	99:103	arg1	outcomes					25:32	outcomes	25:32	outcomes after cardiac surgery	25:54	Contaminated heparin and outcomes after cardiac surgery: a retrospective propensity-matched cohort study.
25162640	0	29	dep	study	99:103	arg1	heparin					13:19	Contaminated heparin	0:19	Contaminated heparin	0:19	Contaminated heparin and outcomes after cardiac surgery: a retrospective propensity-matched cohort study.
25162640	0	30	theme	Contaminated	0:11	arg1	heparin					13:19	Contaminated heparin	0:19	Contaminated heparin	0:19	Contaminated heparin and outcomes after cardiac surgery: a retrospective propensity-matched cohort study.
25162640	9	31	dep	3.5	1627:1629	arg1	to					1624:1625	to	1624:1625	to	1624:1625	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	5	32	theme	logistic	936:943	arg1	regression					945:954	logistic regression	936:954	logistic regression	936:954	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	8	33	theme	<	1427:1427	arg1	P					1425:1425	P < 0.001	1425:1433	P < 0.001	1425:1433	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	11	34	theme	cardiac	2047:2053	arg1	surgery					2055:2061	cardiac surgery	2047:2061	cardiac surgery	2047:2061	CONCLUSIONS The results from this single center study suggest the possibility that contaminated heparin might have contributed to serious morbidity following cardiac surgery.
25162640	3	35	from	study	588:592	arg1	years					692:696	the two following years	674:696	the two following years	674:696	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	3	35	from	study	588:592	arg1	preceding					661:669	preceding	661:669	preceding	661:669	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	2	36	theme	intensive	395:403	arg1	ICU					416:418	ICU	416:418	ICU	416:418	We tested the hypothesis that CH was associated with serious morbidity, mortality, intensive care unit (ICU) stay and heparin-induced thrombocytopenia following adult cardiac surgery.
25162640	2	36	theme	intensive	395:403	arg1	unit					410:413	intensive care unit	395:413	intensive care unit (ICU) stay	395:424	We tested the hypothesis that CH was associated with serious morbidity, mortality, intensive care unit (ICU) stay and heparin-induced thrombocytopenia following adult cardiac surgery.
25162640	1	37	theme	anaphylactoid	287:299	arg1	reactions					301:309	anaphylactoid reactions	287:309	anaphylactoid reactions	287:309	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	2	38	theme	heparin-induced	430:444	arg1	thrombocytopenia					446:461	heparin-induced thrombocytopenia	430:461	heparin-induced thrombocytopenia	430:461	We tested the hypothesis that CH was associated with serious morbidity, mortality, intensive care unit (ICU) stay and heparin-induced thrombocytopenia following adult cardiac surgery.
25162640	9	39	theme	hazard	1764:1769	arg1	ratio					1771:1775	an estimated hazard ratio	1751:1775	an estimated hazard ratio for CH of 1.2	1751:1789	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	2	40	dep	unit	410:413	arg1	stay					421:424	stay	421:424	stay	421:424	We tested the hypothesis that CH was associated with serious morbidity, mortality, intensive care unit (ICU) stay and heparin-induced thrombocytopenia following adult cardiac surgery.
25162640	5	41	theme	adverse	1028:1034	arg1	outcome					1036:1042	the composite adverse outcome	1014:1042	the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest)	1014:1111	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	8	42	theme	exposed	1348:1354	arg1	cohort					1356:1361	the exposed cohort	1344:1361	the exposed cohort	1344:1361	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	4	43	theme	Surgeons	789:796	arg1	Database					807:814	Thoracic Surgeons National Database	780:814	Thoracic Surgeons National Database	780:814	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	8	44	dep	%	1422:1422	arg1	to					1415:1416	to	1415:1416	to	1415:1416	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	5	45	theme	CH	1008:1009	arg1	effect					998:1003	the independent effect	982:1003	the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality	982:1128	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	8	46	theme	2.0	1465:1467	arg1	ratio					1449:1453	an odds ratio	1441:1453	an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001)	1441:1499	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	3	47	theme	following	682:690	arg1	years					692:696	the two following years	674:696	the two following years	674:696	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	9	48	dep	range	1703:1707	arg1	to					1715:1716	to	1715:1716	to	1715:1716	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	1	49	theme	oversulfated	227:238	arg1	sulfate					252:258	oversulfated chondroitin sulfate	227:258	oversulfated chondroitin sulfate	227:258	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	9	50	dep	1.4	1807:1809	arg1	to					1804:1805	to	1804:1805	to	1804:1805	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	3	51	theme	equivalent	626:635	arg1	frame					642:646	the equivalent time frame	622:646	the equivalent time frame in the three preceding or the two following years	622:696	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	11	52	theme	contaminated	1972:1983	arg1	heparin					1985:1991	contaminated heparin	1972:1991	contaminated heparin	1972:1991	CONCLUSIONS The results from this single center study suggest the possibility that contaminated heparin might have contributed to serious morbidity following cardiac surgery.
25162640	3	53	theme	single	532:537	arg1	study					588:592	a single center, retrospective, propensity-matched cohort study	530:592	study	588:592	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	9	54	theme	=	1635:1635	arg1	P					1633:1633	P = 0.1	1633:1639	P = 0.1	1633:1639	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	55	dep	longer	1667:1672	arg1	%					1794:1794	95% CI	1792:1797	95% CI	1792:1797	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	5	56	from	effect	998:1003	arg1	outcome					1036:1042	the composite adverse outcome	1014:1042	the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest)	1014:1111	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	5	56	from	effect	998:1003	arg1	mortality					1120:1128	mortality	1120:1128	mortality	1120:1128	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	4	57	theme	data	831:834	arg1	prospective					850:860	prospective	850:860	prospective	850:860	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	4	57	theme	data	831:834	arg1	collection					836:845	the data collection	827:845	the data collection	827:845	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	4	57	theme	data	831:834	arg1	performed					880:888	performed	880:888	performed by independent investigators	880:917	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	4	57	theme	data	831:834	arg1	standardized					863:874	standardized	863:874	standardized	863:874	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	6	58	theme	Cox	1131:1133	arg1	regression					1135:1144	Cox regression	1131:1144	Cox regression	1131:1144	Cox regression was used to determine the association between CH and ICU length of stay.
25162640	11	59	theme	center	1930:1935	arg1	study					1937:1941	this single center study	1918:1941	this single center study	1918:1941	CONCLUSIONS The results from this single center study suggest the possibility that contaminated heparin might have contributed to serious morbidity following cardiac surgery.
25162640	6	60	used	used	1150:1153	arg2	regression					1135:1144	Cox regression	1131:1144	Cox regression	1131:1144	Cox regression was used to determine the association between CH and ICU length of stay.
25162640	8	61	theme	difference	1384:1393	arg1	%					1422:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	9	62	theme	exposed	1509:1515	arg1	group					1517:1521	the exposed group	1505:1521	the exposed group	1505:1521	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	63	dep	%	1577:1577	arg1	%					1630:1630	-0.4 to 3.5%	1619:1630	-0.4 to 3.5%	1619:1630	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	63	dep	%	1577:1577	arg1	P					1633:1633	P = 0.1	1633:1639	P = 0.1	1633:1639	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	63	dep	%	1577:1577	arg1	%					1608:1608	difference = 2.4%	1592:1608	difference = 2.4%	1592:1608	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	63	dep	%	1577:1577	arg1	%					1613:1613	95% CI	1611:1616	95% CI	1611:1616	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	3	64	theme	cohort	581:586	arg1	study					588:592	a single center, retrospective, propensity-matched cohort study	530:592	study	588:592	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	10	65	from	difference	1836:1845	arg1	counts					1865:1870	nadir platelet counts	1850:1870	nadir platelet counts	1850:1870	There was no difference in nadir platelet counts between cohorts.
25162640	3	66	theme	CH	615:616	arg1	period					605:610	the period	601:610	the period of CH	601:616	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	8	67	theme	%	1405:1405	arg1	%					1422:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	difference = 8.9%; 95% CI 3.6% to 15.1%	1384:1422	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	4	68	theme	Perioperative	699:711	arg1	data					713:716	Perioperative data	699:716	Perioperative data	699:716	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	0	69	theme	retrospective	59:71	arg1	study					99:103	a retrospective propensity-matched cohort study	57:103	a retrospective propensity-matched cohort study	57:103	Contaminated heparin and outcomes after cardiac surgery: a retrospective propensity-matched cohort study.
25162640	11	70	from	results	1905:1911	arg1	CONCLUSIONS					1889:1899	CONCLUSIONS	1889:1899	CONCLUSIONS The results from this single center study	1889:1941	CONCLUSIONS The results from this single center study suggest the possibility that contaminated heparin might have contributed to serious morbidity following cardiac surgery.
25162640	9	71	theme	interquartile	1689:1701	arg1	P = 0.0004					1734:1743	P = 0.0004	1734:1743	P = 0.0004	1734:1743	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	71	theme	interquartile	1689:1701	arg1	S = 3299					1724:1731	S = 3299	1724:1731	S = 3299	1724:1731	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	71	theme	interquartile	1689:1701	arg1	range					1703:1707	interquartile range -26.6 to 79.8	1689:1721	interquartile range -26.6 to 79.8	1689:1721	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	0	72	theme	cohort	92:97	arg1	study					99:103	a retrospective propensity-matched cohort study	57:103	a retrospective propensity-matched cohort study	57:103	Contaminated heparin and outcomes after cardiac surgery: a retrospective propensity-matched cohort study.
25162640	8	73	dep	CI	1474:1475	arg1	0.001					1494:1498	0.001	1494:1498	0.001	1494:1498	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	2	74	theme	adult	473:477	arg1	surgery					487:493	adult cardiac surgery	473:493	adult cardiac surgery	473:493	We tested the hypothesis that CH was associated with serious morbidity, mortality, intensive care unit (ICU) stay and heparin-induced thrombocytopenia following adult cardiac surgery.
25162640	10	75	theme	platelet	1856:1863	arg1	counts					1865:1870	nadir platelet counts	1850:1870	nadir platelet counts	1850:1870	There was no difference in nadir platelet counts between cohorts.
25162640	6	76	dep	length	1203:1208	arg1	stay					1213:1216	stay	1213:1216	stay	1213:1216	Cox regression was used to determine the association between CH and ICU length of stay.
25162640	1	77	dep	contaminated	204:215	arg1	heparin					196:202	the US received heparin	180:202	the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions	180:309	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	1	77	dep	contaminated	204:215	arg1	CH					218:219	CH	218:219	CH	218:219	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	1	78	from	millions	156:163	arg1	heparin					196:202	the US received heparin	180:202	the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions	180:309	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	1	78	from	millions	156:163	arg1	CH					218:219	CH	218:219	CH	218:219	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	4	79	theme	institutional	741:753	arg1	record					755:760	the institutional record	737:760	the institutional record of the Society of Thoracic Surgeons National Database	737:814	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	8	80	dep	2.0	1465:1467	arg1	3.0					1485:1487	3.0	1485:1487	3.0	1485:1487	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	8	80	dep	2.0	1465:1467	arg1	CI					1474:1475	95% CI	1470:1475	95% CI	1470:1475	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	8	80	dep	2.0	1465:1467	arg1	P					1490:1490	P	1490:1490	P	1490:1490	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	8	81	dep	were	1314:1317	arg1	%					1368:1368	20.9%	1364:1368	20.9%	1364:1368	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	8	81	dep	were	1314:1317	arg1	%					1381:1381	12.0%	1377:1381	12.0%	1377:1381	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	5	82	theme	cardiac	1097:1103	arg1	infarction					1056:1065	myocardial infarction	1045:1065	myocardial infarction	1045:1065	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	5	82	theme	cardiac	1097:1103	arg1	arrest					1105:1110	cardiac arrest	1097:1110	cardiac arrest	1097:1110	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	3	83	dep	METHODS	496:502	arg1	We					517:518	We	517:518	We	517:518	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	8	84	theme	adverse	1324:1330	arg1	outcomes					1332:1339	more adverse outcomes	1319:1339	more adverse outcomes	1319:1339	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	2	85	theme	serious	365:371	arg1	morbidity					373:381	serious morbidity	365:381	serious morbidity	365:381	We tested the hypothesis that CH was associated with serious morbidity, mortality, intensive care unit (ICU) stay and heparin-induced thrombocytopenia following adult cardiac surgery.
25162640	3	86	theme	time	637:640	arg1	frame					642:646	the equivalent time frame	622:646	the equivalent time frame in the three preceding or the two following years	622:696	METHODS AND FINDINGS We conducted a single center, retrospective, propensity-matched cohort study during the period of CH and the equivalent time frame in the three preceding or the two following years.
25162640	2	87	theme	care	405:408	arg1	ICU					416:418	ICU	416:418	ICU	416:418	We tested the hypothesis that CH was associated with serious morbidity, mortality, intensive care unit (ICU) stay and heparin-induced thrombocytopenia following adult cardiac surgery.
25162640	2	87	theme	care	405:408	arg1	unit					410:413	intensive care unit	395:413	intensive care unit (ICU) stay	395:424	We tested the hypothesis that CH was associated with serious morbidity, mortality, intensive care unit (ICU) stay and heparin-induced thrombocytopenia following adult cardiac surgery.
25162640	0	88	theme	cardiac	40:46	arg1	surgery					48:54	cardiac surgery	40:54	cardiac surgery	40:54	Contaminated heparin and outcomes after cardiac surgery: a retrospective propensity-matched cohort study.
25162640	8	89	theme	odds	1444:1447	arg1	ratio					1449:1453	an odds ratio	1441:1453	an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001)	1441:1499	There were more adverse outcomes in the exposed cohort (20.9% versus 12.0%; difference = 8.9%; 95% CI 3.6% to 15.1%, P < 0.001) with an odds ratio for CH of 2.0 (95% CI, 1.4 to 3.0, P < 0.001).
25162640	9	90	theme	estimated	1754:1762	arg1	ratio					1771:1775	an estimated hazard ratio	1751:1775	an estimated hazard ratio for CH of 1.2	1751:1789	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	4	91	theme	National	798:805	arg1	Database					807:814	Thoracic Surgeons National Database	780:814	Thoracic Surgeons National Database	780:814	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	4	92	dep	prospective	850:860	arg1	prospective					850:860	prospective	850:860	prospective	850:860	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	4	92	dep	prospective	850:860	arg1	collection					836:845	the data collection	827:845	the data collection	827:845	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	4	92	dep	prospective	850:860	arg1	performed					880:888	performed	880:888	performed by independent investigators	880:917	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	4	92	dep	prospective	850:860	arg1	standardized					863:874	standardized	863:874	standardized	863:874	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	5	93	theme	composite	1018:1026	arg1	outcome					1036:1042	the composite adverse outcome	1014:1042	the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest)	1014:1111	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	11	94	dep	results	1905:1911	arg1	study					1937:1941	this single center study	1918:1941	this single center study	1918:1941	CONCLUSIONS The results from this single center study suggest the possibility that contaminated heparin might have contributed to serious morbidity following cardiac surgery.
25162640	1	95	dep	received	187:194	arg1	US					184:185	US	184:185	US	184:185	BACKGROUND During 2007 and 2008 it is likely that millions of patients in the US received heparin contaminated (CH) with oversulfated chondroitin sulfate, which was associated with anaphylactoid reactions.
25162640	5	96	theme	independent	986:996	arg1	effect					998:1003	the independent effect	982:1003	the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality	982:1128	After matching, logistic regression was performed to evaluate the independent effect of CH on the composite adverse outcome (myocardial infarction, stroke, pneumonia, dialysis, cardiac arrest) and on mortality.
25162640	7	97	theme	matched	1227:1233	arg1	groups					1235:1240	The 1∶5 matched groups	1219:1240	The 1∶5 matched groups	1219:1240	The 1∶5 matched groups included 220 patients potentially exposed to CH and 918 controls.
25162640	4	98	theme	Society	769:775	arg1	record					755:760	the institutional record	737:760	the institutional record of the Society of Thoracic Surgeons National Database	737:814	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
25162640	9	99	from	increase	1551:1558	arg1	mortality					1563:1571	mortality	1563:1571	mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1)	1563:1640	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	99	from	increase	1551:1558	arg1	%					1577:1577	5.9% versus 3.5%	1574:1589	%	1577:1577	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	99	from	increase	1551:1558	arg1	%					1589:1589	5.9% versus 3.5%	1574:1589	%	1589:1589	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	9	100	theme	1.2	1787:1789	arg1	ratio					1771:1775	an estimated hazard ratio	1751:1775	an estimated hazard ratio for CH of 1.2	1751:1789	In the exposed group there was a non-significant increase in mortality (5.9% versus 3.5%, difference = 2.4%; 95% CI, -0.4 to 3.5%, P = 0.1), the median ICU stay was longer by 14.1 hours (interquartile range -26.6 to 79.8, S = 3299, P = 0.0004) with an estimated hazard ratio for CH of 1.2 (95% CI, 1.0 to 1.4, P = 0.04).
25162640	4	101	theme	Thoracic	780:787	arg1	Surgeons					789:796	Thoracic Surgeons	780:796	Thoracic Surgeons National Database	780:814	Perioperative data were obtained from the institutional record of the Society of Thoracic Surgeons National Database, for which the data collection is prospective, standardized and performed by independent investigators.
26908829	5	0	dep	apolipoproteins	785:799	arg1	assessed					802:809	assessed	802:809	assessed by two independent methods	802:836	PEG dramatically shifted the size distribution of HDL and apolipoproteins (assessed by two independent methods), while leaving substantial amounts of reagent in the sample.
26908829	8	1	theme	HDL	1269:1271	arg1	composition					1273:1283	HDL composition	1269:1283	HDL composition	1269:1283	Thus, each of the apoB precipitation methods affected HDL composition and/or size distribution.
26908829	2	2	from	serum	275:279	arg1	precipitated					257:268	precipitated	257:268	precipitated	257:268	To study HDL function, apoB-containing lipoproteins are precipitated from serum.
26908829	6	3	theme	oxidation	964:972	arg1	distribution					921:932	the distribution	917:932	the distribution of cholesterol efflux and LDL oxidation across size fractions	917:994	PEG also changed the distribution of cholesterol efflux and LDL oxidation across size fractions, but not overall efflux across the HDL range.
26908829	0	4	from	effects	4:10	arg1	function					76:83	function	76:83	function	76:83	The effects of apolipoprotein B depletion on HDL subspecies composition and function.
26908829	0	4	from	effects	4:10	arg1	composition					60:70	HDL subspecies composition	45:70	HDL subspecies composition	45:70	The effects of apolipoprotein B depletion on HDL subspecies composition and function.
26908829	5	5	theme	size	756:759	arg1	distribution					761:772	the size distribution	752:772	the size distribution of HDL and apolipoproteins (assessed by two independent methods)	752:837	PEG dramatically shifted the size distribution of HDL and apolipoproteins (assessed by two independent methods), while leaving substantial amounts of reagent in the sample.
26908829	7	6	theme	LipoSep	1091:1097	arg1	IP					1099:1100	LipoSep IP	1091:1100	LipoSep IP	1091:1100	Dextran sulfate/MgCl2, heparin sodium/MnCl2, and LipoSep IP did not change the size distribution of HDL subspecies, but altered the quantity of a subset of apolipoproteins.
26908829	4	7	dep	methods	452:458	arg1	chloride					514:521	dextran sulfate/magnesium chloride	488:521	dextran sulfate/magnesium chloride (MgCl2)	488:529	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	7	dep	methods	452:458	arg1	glycol					474:479	[polyethylene glycol	460:479	[polyethylene glycol (PEG)	460:485	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	7	dep	methods	452:458	arg1	chloride					557:564	heparin sodium/manganese chloride	532:564	heparin sodium/manganese chloride (MnCl2)	532:572	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	7	dep	methods	452:458	arg1	PEG					482:484	PEG	482:484	PEG	482:484	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	7	dep	methods	452:458	arg1	immunoprecipitation					587:605	LipoSep immunoprecipitation	579:605	LipoSep immunoprecipitation (IP)	579:610	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	7	dep	methods	452:458	arg1	MgCl2					524:528	MgCl2	524:528	MgCl2	524:528	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	7	dep	methods	452:458	arg1	IP					608:609	IP	608:609	IP	608:609	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	7	dep	methods	452:458	arg1	MnCl2					567:571	MnCl2	567:571	MnCl2	567:571	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	7	dep	methods	452:458	arg1	methods					452:458	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	7	8	theme	subspecies	1146:1155	arg1	distribution					1126:1137	the size distribution	1117:1137	the size distribution of HDL subspecies	1117:1155	Dextran sulfate/MgCl2, heparin sodium/MnCl2, and LipoSep IP did not change the size distribution of HDL subspecies, but altered the quantity of a subset of apolipoproteins.
26908829	4	9	theme	sulfate/magnesium	496:512	arg1	methods					452:458	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	9	theme	sulfate/magnesium	496:512	arg1	MgCl2					524:528	MgCl2	524:528	MgCl2	524:528	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	9	theme	sulfate/magnesium	496:512	arg1	chloride					514:521	dextran sulfate/magnesium chloride	488:521	dextran sulfate/magnesium chloride (MgCl2)	488:529	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	7	10	theme	heparin	1065:1071	arg1	sodium/MnCl2					1073:1084	heparin sodium/MnCl2	1065:1084	heparin sodium/MnCl2	1065:1084	Dextran sulfate/MgCl2, heparin sodium/MnCl2, and LipoSep IP did not change the size distribution of HDL subspecies, but altered the quantity of a subset of apolipoproteins.
26908829	9	11	theme	apoB	1372:1375	arg1	methods					1387:1393	apoB depletion methods	1372:1393	apoB depletion methods	1372:1393	We conclude that careful evaluation is needed when selecting apoB depletion methods for existing and future bioassays of HDL function.
26908829	7	12	theme	apolipoproteins	1198:1212	arg1	subset					1188:1193	a subset	1186:1193	a subset of apolipoproteins	1186:1212	Dextran sulfate/MgCl2, heparin sodium/MnCl2, and LipoSep IP did not change the size distribution of HDL subspecies, but altered the quantity of a subset of apolipoproteins.
26908829	4	13	dep	function	665:672	arg1	efflux					687:692	cholesterol efflux	675:692	cholesterol efflux	675:692	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	13	dep	function	665:672	arg1	reduction					698:706	reduction	698:706	reduction of LDL oxidation	698:723	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	6	14	theme	efflux	949:954	arg1	distribution					921:932	the distribution	917:932	the distribution of cholesterol efflux and LDL oxidation across size fractions	917:994	PEG also changed the distribution of cholesterol efflux and LDL oxidation across size fractions, but not overall efflux across the HDL range.
26908829	9	15	theme	function	1436:1443	arg1	bioassays					1419:1427	existing and future bioassays	1399:1427	existing and future bioassays of HDL function	1399:1443	We conclude that careful evaluation is needed when selecting apoB depletion methods for existing and future bioassays of HDL function.
26908829	6	16	theme	overall	1005:1011	arg1	efflux					1013:1018	overall efflux	1005:1018	overall efflux	1005:1018	PEG also changed the distribution of cholesterol efflux and LDL oxidation across size fractions, but not overall efflux across the HDL range.
26908829	9	17	theme	depletion	1377:1385	arg1	methods					1387:1393	apoB depletion methods	1372:1393	apoB depletion methods	1372:1393	We conclude that careful evaluation is needed when selecting apoB depletion methods for existing and future bioassays of HDL function.
26908829	9	18	theme	existing	1399:1406	arg1	bioassays					1419:1427	existing and future bioassays	1399:1427	existing and future bioassays of HDL function	1399:1443	We conclude that careful evaluation is needed when selecting apoB depletion methods for existing and future bioassays of HDL function.
26908829	1	19	theme	robust	140:145	arg1	function					117:124	HDL cholesterol (HDL-C) efflux function	86:124	HDL cholesterol (HDL-C) efflux function	86:124	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	1	19	theme	robust	140:145	arg1	biomarker					147:155	a more robust biomarker	133:155	a more robust biomarker of coronary artery disease risk than HDL-C	133:198	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	7	20	theme	HDL	1142:1144	arg1	subspecies					1146:1155	HDL subspecies	1142:1155	HDL subspecies	1142:1155	Dextran sulfate/MgCl2, heparin sodium/MnCl2, and LipoSep IP did not change the size distribution of HDL subspecies, but altered the quantity of a subset of apolipoproteins.
26908829	8	21	theme	size	1292:1295	arg1	distribution					1297:1308	size distribution	1292:1308	size distribution	1292:1308	Thus, each of the apoB precipitation methods affected HDL composition and/or size distribution.
26908829	7	22	theme	Dextran	1042:1048	arg1	sulfate/MgCl2					1050:1062	Dextran sulfate/MgCl2	1042:1062	Dextran sulfate/MgCl2	1042:1062	Dextran sulfate/MgCl2, heparin sodium/MnCl2, and LipoSep IP did not change the size distribution of HDL subspecies, but altered the quantity of a subset of apolipoproteins.
26908829	4	23	theme	subspecies	620:629	arg1	composition					631:641	HDL subspecies composition	616:641	HDL subspecies composition	616:641	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	5	24	theme	HDL	777:779	arg1	distribution					761:772	the size distribution	752:772	the size distribution of HDL and apolipoproteins (assessed by two independent methods)	752:837	PEG dramatically shifted the size distribution of HDL and apolipoproteins (assessed by two independent methods), while leaving substantial amounts of reagent in the sample.
26908829	6	25	theme	cholesterol	937:947	arg1	efflux					949:954	cholesterol efflux	937:954	cholesterol efflux	937:954	PEG also changed the distribution of cholesterol efflux and LDL oxidation across size fractions, but not overall efflux across the HDL range.
26908829	4	26	theme	LDL	711:713	arg1	oxidation					715:723	LDL oxidation	711:723	LDL oxidation	711:723	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	7	27	theme	size	1121:1124	arg1	distribution					1126:1137	the size distribution	1117:1137	the size distribution of HDL subspecies	1117:1155	Dextran sulfate/MgCl2, heparin sodium/MnCl2, and LipoSep IP did not change the size distribution of HDL subspecies, but altered the quantity of a subset of apolipoproteins.
26908829	4	28	theme	HDL	616:618	arg1	composition					631:641	HDL subspecies composition	616:641	HDL subspecies composition	616:641	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	29	theme	sodium/manganese	540:555	arg1	methods					452:458	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	29	theme	sodium/manganese	540:555	arg1	MnCl2					567:571	MnCl2	567:571	MnCl2	567:571	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	29	theme	sodium/manganese	540:555	arg1	chloride					557:564	heparin sodium/manganese chloride	532:564	heparin sodium/manganese chloride (MnCl2)	532:572	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	6	30	theme	LDL	960:962	arg1	oxidation					964:972	LDL oxidation	960:972	LDL oxidation	960:972	PEG also changed the distribution of cholesterol efflux and LDL oxidation across size fractions, but not overall efflux across the HDL range.
26908829	1	31	theme	HDL	86:88	arg1	HDL-C					103:107	HDL-C	103:107	HDL-C	103:107	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	1	31	theme	HDL	86:88	arg1	cholesterol					90:100	HDL cholesterol	86:100	HDL cholesterol (HDL-C) efflux function	86:124	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	1	32	theme	coronary	160:167	arg1	risk					184:187	coronary artery disease risk	160:187	coronary artery disease risk than HDL-C	160:198	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	0	33	theme	apolipoprotein	15:28	arg1	depletion					32:40	apolipoprotein B depletion	15:40	apolipoprotein B depletion	15:40	The effects of apolipoprotein B depletion on HDL subspecies composition and function.
26908829	4	34	theme	heparin	532:538	arg1	methods					452:458	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	34	theme	heparin	532:538	arg1	MnCl2					567:571	MnCl2	567:571	MnCl2	567:571	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	34	theme	heparin	532:538	arg1	chloride					557:564	heparin sodium/manganese chloride	532:564	heparin sodium/manganese chloride (MnCl2)	532:572	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	1	35	theme	cholesterol	90:100	arg1	function					117:124	HDL cholesterol (HDL-C) efflux function	86:124	HDL cholesterol (HDL-C) efflux function	86:124	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	1	35	theme	cholesterol	90:100	arg1	biomarker					147:155	a more robust biomarker	133:155	a more robust biomarker of coronary artery disease risk than HDL-C	133:198	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	1	36	theme	artery	169:174	arg1	risk					184:187	coronary artery disease risk	160:187	coronary artery disease risk than HDL-C	160:198	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	4	37	theme	precipitation	438:450	arg1	chloride					557:564	heparin sodium/manganese chloride	532:564	heparin sodium/manganese chloride (MnCl2)	532:572	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	37	theme	precipitation	438:450	arg1	immunoprecipitation					587:605	LipoSep immunoprecipitation	579:605	LipoSep immunoprecipitation (IP)	579:610	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	37	theme	precipitation	438:450	arg1	glycol					474:479	[polyethylene glycol	460:479	[polyethylene glycol (PEG)	460:485	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	37	theme	precipitation	438:450	arg1	methods					452:458	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	37	theme	precipitation	438:450	arg1	chloride					514:521	dextran sulfate/magnesium chloride	488:521	dextran sulfate/magnesium chloride (MgCl2)	488:529	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	38	theme	oxidation	715:723	arg1	efflux					687:692	cholesterol efflux	675:692	cholesterol efflux	675:692	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	38	theme	oxidation	715:723	arg1	reduction					698:706	reduction	698:706	reduction of LDL oxidation	698:723	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	1	39	theme	disease	176:182	arg1	risk					184:187	coronary artery disease risk	160:187	coronary artery disease risk than HDL-C	160:198	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	0	40	theme	depletion	32:40	arg1	effects					4:10	The effects	0:10	The effects of apolipoprotein B depletion on HDL subspecies composition and function	0:83	The effects of apolipoprotein B depletion on HDL subspecies composition and function.
26908829	4	41	theme	apoB	433:436	arg1	chloride					557:564	heparin sodium/manganese chloride	532:564	heparin sodium/manganese chloride (MnCl2)	532:572	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	41	theme	apoB	433:436	arg1	immunoprecipitation					587:605	LipoSep immunoprecipitation	579:605	LipoSep immunoprecipitation (IP)	579:610	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	41	theme	apoB	433:436	arg1	glycol					474:479	[polyethylene glycol	460:479	[polyethylene glycol (PEG)	460:485	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	41	theme	apoB	433:436	arg1	methods					452:458	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	41	theme	apoB	433:436	arg1	chloride					514:521	dextran sulfate/magnesium chloride	488:521	dextran sulfate/magnesium chloride (MgCl2)	488:529	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	8	42	theme	apoB	1233:1236	arg1	methods					1252:1258	the apoB precipitation methods	1229:1258	the apoB precipitation methods	1229:1258	Thus, each of the apoB precipitation methods affected HDL composition and/or size distribution.
26908829	1	43	theme	risk	184:187	arg1	function					117:124	HDL cholesterol (HDL-C) efflux function	86:124	HDL cholesterol (HDL-C) efflux function	86:124	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	1	43	theme	risk	184:187	arg1	biomarker					147:155	a more robust biomarker	133:155	a more robust biomarker of coronary artery disease risk than HDL-C	133:198	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	0	44	theme	B	30:30	arg1	depletion					32:40	apolipoprotein B depletion	15:40	apolipoprotein B depletion	15:40	The effects of apolipoprotein B depletion on HDL subspecies composition and function.
26908829	3	45	dep	subspecies	321:330	arg1	subspecies					321:330	HDL subspecies composition and function	317:355	HDL subspecies composition and function	317:355	Whether apoB precipitation affects HDL subspecies composition and function has not been thoroughly investigated.
26908829	3	45	dep	subspecies	321:330	arg1	function					348:355	function	348:355	function	348:355	Whether apoB precipitation affects HDL subspecies composition and function has not been thoroughly investigated.
26908829	3	45	dep	subspecies	321:330	arg1	composition					332:342	composition	332:342	composition	332:342	Whether apoB precipitation affects HDL subspecies composition and function has not been thoroughly investigated.
26908829	4	46	theme	[polyethylene	460:472	arg1	PEG					482:484	PEG	482:484	PEG	482:484	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	46	theme	[polyethylene	460:472	arg1	glycol					474:479	[polyethylene glycol	460:479	[polyethylene glycol (PEG)	460:485	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	46	theme	[polyethylene	460:472	arg1	methods					452:458	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	5	47	theme	apolipoproteins	785:799	arg1	distribution					761:772	the size distribution	752:772	the size distribution of HDL and apolipoproteins (assessed by two independent methods)	752:837	PEG dramatically shifted the size distribution of HDL and apolipoproteins (assessed by two independent methods), while leaving substantial amounts of reagent in the sample.
26908829	5	48	theme	independent	818:828	arg1	methods					830:836	two independent methods	814:836	two independent methods	814:836	PEG dramatically shifted the size distribution of HDL and apolipoproteins (assessed by two independent methods), while leaving substantial amounts of reagent in the sample.
26908829	0	49	theme	HDL	45:47	arg1	composition					60:70	HDL subspecies composition	45:70	HDL subspecies composition	45:70	The effects of apolipoprotein B depletion on HDL subspecies composition and function.
26908829	4	50	theme	methods	452:458	arg1	effects					410:416	the effects	406:416	the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation)	406:724	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	5	51	theme	reagent	877:883	arg1	amounts					866:872	substantial amounts	854:872	substantial amounts of reagent	854:883	PEG dramatically shifted the size distribution of HDL and apolipoproteins (assessed by two independent methods), while leaving substantial amounts of reagent in the sample.
26908829	5	51	theme	reagent	877:883	arg1	reagent					877:883	reagent	877:883	reagent	877:883	PEG dramatically shifted the size distribution of HDL and apolipoproteins (assessed by two independent methods), while leaving substantial amounts of reagent in the sample.
26908829	3	52	theme	apoB	290:293	arg1	precipitation					295:307	apoB precipitation	290:307	apoB precipitation	290:307	Whether apoB precipitation affects HDL subspecies composition and function has not been thoroughly investigated.
26908829	4	53	theme	cholesterol	675:685	arg1	efflux					687:692	cholesterol efflux	675:692	cholesterol efflux	675:692	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	2	54	theme	apoB-containing	224:238	arg1	lipoproteins					240:251	apoB-containing lipoproteins	224:251	apoB-containing lipoproteins	224:251	To study HDL function, apoB-containing lipoproteins are precipitated from serum.
26908829	1	55	theme	efflux	110:115	arg1	function					117:124	HDL cholesterol (HDL-C) efflux function	86:124	HDL cholesterol (HDL-C) efflux function	86:124	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	1	55	theme	efflux	110:115	arg1	biomarker					147:155	a more robust biomarker	133:155	a more robust biomarker of coronary artery disease risk than HDL-C	133:198	HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C.
26908829	9	56	theme	careful	1328:1334	arg1	evaluation					1336:1345	careful evaluation	1328:1345	careful evaluation	1328:1345	We conclude that careful evaluation is needed when selecting apoB depletion methods for existing and future bioassays of HDL function.
26908829	4	57	from	effects	410:416	arg1	function					665:672	function	665:672	function (cholesterol efflux and reduction of LDL oxidation)	665:724	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	57	from	effects	410:416	arg1	composition					631:641	HDL subspecies composition	616:641	HDL subspecies composition	616:641	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	57	from	effects	410:416	arg1	apolipoproteins					644:658	apolipoproteins	644:658	apolipoproteins	644:658	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	58	theme	common	426:431	arg1	chloride					557:564	heparin sodium/manganese chloride	532:564	heparin sodium/manganese chloride (MnCl2)	532:572	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	58	theme	common	426:431	arg1	immunoprecipitation					587:605	LipoSep immunoprecipitation	579:605	LipoSep immunoprecipitation (IP)	579:610	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	58	theme	common	426:431	arg1	glycol					474:479	[polyethylene glycol	460:479	[polyethylene glycol (PEG)	460:485	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	58	theme	common	426:431	arg1	methods					452:458	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	58	theme	common	426:431	arg1	chloride					514:521	dextran sulfate/magnesium chloride	488:521	dextran sulfate/magnesium chloride (MgCl2)	488:529	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	8	59	theme	precipitation	1238:1250	arg1	methods					1252:1258	the apoB precipitation methods	1229:1258	the apoB precipitation methods	1229:1258	Thus, each of the apoB precipitation methods affected HDL composition and/or size distribution.
26908829	4	60	theme	LipoSep	579:585	arg1	immunoprecipitation					587:605	LipoSep immunoprecipitation	579:605	LipoSep immunoprecipitation (IP)	579:610	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	60	theme	LipoSep	579:585	arg1	methods					452:458	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	60	theme	LipoSep	579:585	arg1	IP					608:609	IP	608:609	IP	608:609	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	3	61	theme	HDL	317:319	arg1	subspecies					321:330	HDL subspecies composition and function	317:355	HDL subspecies composition and function	317:355	Whether apoB precipitation affects HDL subspecies composition and function has not been thoroughly investigated.
26908829	3	61	theme	HDL	317:319	arg1	function					348:355	function	348:355	function	348:355	Whether apoB precipitation affects HDL subspecies composition and function has not been thoroughly investigated.
26908829	3	61	theme	HDL	317:319	arg1	composition					332:342	composition	332:342	composition	332:342	Whether apoB precipitation affects HDL subspecies composition and function has not been thoroughly investigated.
26908829	9	62	theme	HDL	1432:1434	arg1	function					1436:1443	HDL function	1432:1443	HDL function	1432:1443	We conclude that careful evaluation is needed when selecting apoB depletion methods for existing and future bioassays of HDL function.
26908829	9	63	theme	future	1412:1417	arg1	bioassays					1419:1427	existing and future bioassays	1399:1427	existing and future bioassays of HDL function	1399:1443	We conclude that careful evaluation is needed when selecting apoB depletion methods for existing and future bioassays of HDL function.
26908829	7	64	theme	subset	1188:1193	arg1	quantity					1174:1181	the quantity	1170:1181	the quantity of a subset of apolipoproteins	1170:1212	Dextran sulfate/MgCl2, heparin sodium/MnCl2, and LipoSep IP did not change the size distribution of HDL subspecies, but altered the quantity of a subset of apolipoproteins.
26908829	6	65	theme	HDL	1031:1033	arg1	range					1035:1039	the HDL range	1027:1039	the HDL range	1027:1039	PEG also changed the distribution of cholesterol efflux and LDL oxidation across size fractions, but not overall efflux across the HDL range.
26908829	6	66	theme	size	981:984	arg1	fractions					986:994	size fractions	981:994	size fractions	981:994	PEG also changed the distribution of cholesterol efflux and LDL oxidation across size fractions, but not overall efflux across the HDL range.
26908829	0	67	theme	subspecies	49:58	arg1	composition					60:70	HDL subspecies composition	45:70	HDL subspecies composition	45:70	The effects of apolipoprotein B depletion on HDL subspecies composition and function.
26908829	4	68	theme	dextran	488:494	arg1	methods					452:458	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)]	421:611	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	68	theme	dextran	488:494	arg1	MgCl2					524:528	MgCl2	524:528	MgCl2	524:528	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	4	68	theme	dextran	488:494	arg1	chloride					514:521	dextran sulfate/magnesium chloride	488:521	dextran sulfate/magnesium chloride (MgCl2)	488:529	We studied the effects of four common apoB precipitation methods [polyethylene glycol (PEG), dextran sulfate/magnesium chloride (MgCl2), heparin sodium/manganese chloride (MnCl2), and LipoSep immunoprecipitation (IP)] on HDL subspecies composition, apolipoproteins, and function (cholesterol efflux and reduction of LDL oxidation).
26908829	2	69	theme	HDL	210:212	arg1	function					214:221	HDL function	210:221	HDL function	210:221	To study HDL function, apoB-containing lipoproteins are precipitated from serum.
26908829	5	70	theme	substantial	854:864	arg1	amounts					866:872	substantial amounts	854:872	substantial amounts of reagent	854:883	PEG dramatically shifted the size distribution of HDL and apolipoproteins (assessed by two independent methods), while leaving substantial amounts of reagent in the sample.
26908829	5	70	theme	substantial	854:864	arg1	reagent					877:883	reagent	877:883	reagent	877:883	PEG dramatically shifted the size distribution of HDL and apolipoproteins (assessed by two independent methods), while leaving substantial amounts of reagent in the sample.
28691691	0	0	theme	skeletal	60:67	arg1	application					69:79	skeletal application	60:79	skeletal application	60:79	Development of a clay based bioink for 3D cell printing for skeletal application.
28691691	10	1	theme	alginate-methylcellulose	1366:1389	arg1	blend					1391:1395	an alginate-methylcellulose blend	1363:1395	an alginate-methylcellulose blend in the absence of Laponite	1363:1422	We demonstrate that the release of both growth factors significantly changed to a more sustained profile by inclusion of Laponite in comparison to an alginate-methylcellulose blend in the absence of Laponite.
28691691	7	2	theme	plotted	973:979	arg1	constructs					981:990	the plotted constructs	969:990	the plotted constructs	969:990	Nevertheless, shape of the plotted constructs was preserved even over longer cultivation periods.
28691691	10	3	theme	growth	1256:1261	arg1	factors					1263:1269	both growth factors	1251:1269	both growth factors	1251:1269	We demonstrate that the release of both growth factors significantly changed to a more sustained profile by inclusion of Laponite in comparison to an alginate-methylcellulose blend in the absence of Laponite.
28691691	8	4	theme	favourable	1070:1079	arg1	properties					1095:1104	its favourable drug delivery properties	1066:1104	its favourable drug delivery properties	1066:1104	Laponite is known for its favourable drug delivery properties.
28691691	10	5	theme	Laponite	1337:1344	arg1	inclusion					1324:1332	inclusion	1324:1332	inclusion of Laponite in comparison to an alginate-methylcellulose blend in the absence of Laponite	1324:1422	We demonstrate that the release of both growth factors significantly changed to a more sustained profile by inclusion of Laponite in comparison to an alginate-methylcellulose blend in the absence of Laponite.
28691691	1	6	theme	promising	150:158	arg1	approach					160:167	a promising approach	148:167	a promising approach on the route to patient-specific or complex tissue-engineered constructs	148:240	Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs.
28691691	11	7	theme	active	1583:1588	arg1	factors					1612:1618	growth factors	1605:1618	growth factors	1605:1618	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	11	7	theme	active	1583:1588	arg1	agents					1590:1595	biologically active agents	1570:1595	biologically active agents such as growth factors	1570:1618	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	5	8	theme	mesenchymal	704:714	arg1	cells					721:725	printed immortalised human mesenchymal stem cells	677:725	printed immortalised human mesenchymal stem cells	677:725	Following extrusion, approximately 70%-75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs.
28691691	9	9	theme	growth	1173:1178	arg1	factor					1180:1185	vascular endothelial growth factor	1152:1185	vascular endothelial growth factor	1152:1185	Two model proteins, bovine serum albumin and vascular endothelial growth factor were loaded into the bioink.
28691691	5	10	theme	stem	716:719	arg1	cells					721:725	printed immortalised human mesenchymal stem cells	677:725	printed immortalised human mesenchymal stem cells	677:725	Following extrusion, approximately 70%-75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs.
28691691	9	11	theme	bovine	1127:1132	arg1	albumin					1140:1146	bovine serum albumin	1127:1146	bovine serum albumin	1127:1146	Two model proteins, bovine serum albumin and vascular endothelial growth factor were loaded into the bioink.
28691691	2	12	theme	print	279:283	arg1	structures					285:294	print structures	279:294	print structures with both, high shape fidelity and cell vitality	279:343	However, it is still challenging to print structures with both, high shape fidelity and cell vitality.
28691691	10	13	theme	sustained	1303:1311	arg1	profile					1313:1319	a more sustained profile	1296:1319	a more sustained profile	1296:1319	We demonstrate that the release of both growth factors significantly changed to a more sustained profile by inclusion of Laponite in comparison to an alginate-methylcellulose blend in the absence of Laponite.
28691691	3	14	theme	method	462:467	arg1	plotting					472:479	the extrusion-based method 3D plotting	442:479	the extrusion-based method 3D plotting	442:479	Herein, we used a synthetic nanosilicate clay, called Laponite, to build up scaffolds utilising the extrusion-based method 3D plotting.
28691691	5	15	theme	cells	721:725	arg1	cells					721:725	printed immortalised human mesenchymal stem cells	677:725	printed immortalised human mesenchymal stem cells	677:725	Following extrusion, approximately 70%-75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs.
28691691	5	15	theme	cells	721:725	arg1	%					668:668	approximately 70%	652:668	approximately 70%-75% of printed immortalised human mesenchymal stem cells	652:725	Following extrusion, approximately 70%-75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs.
28691691	3	16	theme	3D	469:470	arg1	plotting					472:479	the extrusion-based method 3D plotting	442:479	the extrusion-based method 3D plotting	442:479	Herein, we used a synthetic nanosilicate clay, called Laponite, to build up scaffolds utilising the extrusion-based method 3D plotting.
28691691	4	17	theme	high	607:610	arg1	fidelity					621:628	high printing fidelity	607:628	high printing fidelity	607:628	By blending with alginate and methylcellulose, a bioink was developed which allowed easy extrusion, achieving scaffolds with high printing fidelity.
28691691	4	18	theme	easy	566:569	arg1	extrusion					571:579	easy extrusion	566:579	easy extrusion	566:579	By blending with alginate and methylcellulose, a bioink was developed which allowed easy extrusion, achieving scaffolds with high printing fidelity.
28691691	11	19	theme	shape	1510:1514	arg1	fidelity					1516:1523	shape fidelity	1510:1523	shape fidelity	1510:1523	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	6	20	theme	scaffolds	839:847	arg1	properties					825:834	Mechanical properties	814:834	Mechanical properties of scaffolds comprised of the composite bioink	814:881	Mechanical properties of scaffolds comprised of the composite bioink decreased over time when stored under cell culture conditions.
28691691	10	21	from	inclusion	1324:1332	arg1	comparison					1349:1358	comparison	1349:1358	comparison to an alginate-methylcellulose blend in the absence of Laponite	1349:1422	We demonstrate that the release of both growth factors significantly changed to a more sustained profile by inclusion of Laponite in comparison to an alginate-methylcellulose blend in the absence of Laponite.
28691691	6	22	theme	composite	866:874	arg1	bioink					876:881	the composite bioink	862:881	the composite bioink	862:881	Mechanical properties of scaffolds comprised of the composite bioink decreased over time when stored under cell culture conditions.
28691691	2	23	theme	high	307:310	arg1	fidelity					318:325	high shape fidelity	307:325	high shape fidelity	307:325	However, it is still challenging to print structures with both, high shape fidelity and cell vitality.
28691691	3	24	theme	synthetic	364:372	arg1	clay					387:390	a synthetic nanosilicate clay	362:390	a synthetic nanosilicate clay	362:390	Herein, we used a synthetic nanosilicate clay, called Laponite, to build up scaffolds utilising the extrusion-based method 3D plotting.
28691691	10	25	theme	Laponite	1415:1422	arg1	absence					1404:1410	the absence	1400:1410	the absence of Laponite	1400:1422	We demonstrate that the release of both growth factors significantly changed to a more sustained profile by inclusion of Laponite in comparison to an alginate-methylcellulose blend in the absence of Laponite.
28691691	11	26	theme	controlled	1548:1557	arg1	release					1559:1565	controlled release	1548:1565	controlled release of biologically active agents such as growth factors	1548:1618	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	11	27	theme	clay	1461:1464	arg1	addition					1437:1444	addition	1437:1444	addition of a synthetic clay, Laponite, improved printability,	1437:1498	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	0	28	theme	clay	17:20	arg1	based					22:26	a clay based	15:26	a clay based	15:26	Development of a clay based bioink for 3D cell printing for skeletal application.
28691691	5	29	dep	%	668:668	arg1	%					672:672	-75%	669:672	approximately 70%-75% of printed immortalised human mesenchymal stem cells	652:725	Following extrusion, approximately 70%-75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs.
28691691	3	30	used	used	357:360	arg2	we					354:355	we	354:355	we	354:355	Herein, we used a synthetic nanosilicate clay, called Laponite, to build up scaffolds utilising the extrusion-based method 3D plotting.
28691691	1	31	theme	Three-dimensional	82:98	arg1	printing					100:107	Three-dimensional printing	82:107	Three-dimensional printing of cell-laden hydrogels	82:131	Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs.
28691691	4	32	theme	printing	612:619	arg1	fidelity					621:628	high printing fidelity	607:628	high printing fidelity	607:628	By blending with alginate and methylcellulose, a bioink was developed which allowed easy extrusion, achieving scaffolds with high printing fidelity.
28691691	5	33	theme	printed	677:683	arg1	cells					721:725	printed immortalised human mesenchymal stem cells	677:725	printed immortalised human mesenchymal stem cells	677:725	Following extrusion, approximately 70%-75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs.
28691691	5	34	theme	plotted	794:800	arg1	constructs					802:811	the plotted constructs	790:811	the plotted constructs	790:811	Following extrusion, approximately 70%-75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs.
28691691	9	35	theme	model	1111:1115	arg1	proteins					1117:1124	Two model proteins	1107:1124	Two model proteins	1107:1124	Two model proteins, bovine serum albumin and vascular endothelial growth factor were loaded into the bioink.
28691691	1	36	theme	patient-specific	185:200	arg1	constructs					231:240	patient-specific or complex tissue-engineered constructs	185:240	patient-specific or complex tissue-engineered constructs	185:240	Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs.
28691691	10	37	from	blend	1391:1395	arg1	absence					1404:1410	the absence	1400:1410	the absence of Laponite	1400:1422	We demonstrate that the release of both growth factors significantly changed to a more sustained profile by inclusion of Laponite in comparison to an alginate-methylcellulose blend in the absence of Laponite.
28691691	5	38	theme	immortalised	685:696	arg1	cells					721:725	printed immortalised human mesenchymal stem cells	677:725	printed immortalised human mesenchymal stem cells	677:725	Following extrusion, approximately 70%-75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs.
28691691	11	39	theme	synthetic	1451:1459	arg1	Laponite					1467:1474	Laponite	1467:1474	Laponite	1467:1474	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	11	39	theme	synthetic	1451:1459	arg1	printability					1486:1497	improved printability	1477:1497	improved printability	1477:1497	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	11	39	theme	synthetic	1451:1459	arg1	clay					1461:1464	a synthetic clay	1449:1464	a synthetic clay	1449:1464	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	0	40	theme	based	22:26	arg1	Development					0:10	Development	0:10	Development of a clay based	0:26	Development of a clay based bioink for 3D cell printing for skeletal application.
28691691	5	41	theme	human	698:702	arg1	cells					721:725	printed immortalised human mesenchymal stem cells	677:725	printed immortalised human mesenchymal stem cells	677:725	Following extrusion, approximately 70%-75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs.
28691691	4	42	with	scaffolds	592:600	arg1	fidelity					621:628	high printing fidelity	607:628	high printing fidelity	607:628	By blending with alginate and methylcellulose, a bioink was developed which allowed easy extrusion, achieving scaffolds with high printing fidelity.
28691691	1	43	theme	complex	205:211	arg1	constructs					231:240	patient-specific or complex tissue-engineered constructs	185:240	patient-specific or complex tissue-engineered constructs	185:240	Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs.
28691691	3	44	theme	nanosilicate	374:385	arg1	clay					387:390	a synthetic nanosilicate clay	362:390	a synthetic nanosilicate clay	362:390	Herein, we used a synthetic nanosilicate clay, called Laponite, to build up scaffolds utilising the extrusion-based method 3D plotting.
28691691	0	45	theme	cell	42:45	arg1	printing					47:54	3D cell printing	39:54	3D cell printing for skeletal application	39:79	Development of a clay based bioink for 3D cell printing for skeletal application.
28691691	7	46	theme	longer	1016:1021	arg1	periods					1035:1041	longer cultivation periods	1016:1041	longer cultivation periods	1016:1041	Nevertheless, shape of the plotted constructs was preserved even over longer cultivation periods.
28691691	2	47	theme	cell	331:334	arg1	vitality					336:343	cell vitality	331:343	cell vitality	331:343	However, it is still challenging to print structures with both, high shape fidelity and cell vitality.
28691691	8	48	theme	delivery	1086:1093	arg1	properties					1095:1104	its favourable drug delivery properties	1066:1104	its favourable drug delivery properties	1066:1104	Laponite is known for its favourable drug delivery properties.
28691691	6	49	theme	culture	926:932	arg1	conditions					934:943	cell culture conditions	921:943	cell culture conditions	921:943	Mechanical properties of scaffolds comprised of the composite bioink decreased over time when stored under cell culture conditions.
28691691	8	50	theme	drug	1081:1084	arg1	properties					1095:1104	its favourable drug delivery properties	1066:1104	its favourable drug delivery properties	1066:1104	Laponite is known for its favourable drug delivery properties.
28691691	9	51	theme	serum	1134:1138	arg1	albumin					1140:1146	bovine serum albumin	1127:1146	bovine serum albumin	1127:1146	Two model proteins, bovine serum albumin and vascular endothelial growth factor were loaded into the bioink.
28691691	1	52	theme	cell-laden	112:121	arg1	hydrogels					123:131	cell-laden hydrogels	112:131	cell-laden hydrogels	112:131	Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs.
28691691	11	53	theme	improved	1477:1484	arg1	printability					1486:1497	improved printability	1477:1497	improved printability	1477:1497	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	11	53	theme	improved	1477:1484	arg1	clay					1461:1464	a synthetic clay	1449:1464	a synthetic clay	1449:1464	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	6	54	theme	cell	921:924	arg1	conditions					934:943	cell culture conditions	921:943	cell culture conditions	921:943	Mechanical properties of scaffolds comprised of the composite bioink decreased over time when stored under cell culture conditions.
28691691	7	55	theme	cultivation	1023:1033	arg1	periods					1035:1041	longer cultivation periods	1016:1041	longer cultivation periods	1016:1041	Nevertheless, shape of the plotted constructs was preserved even over longer cultivation periods.
28691691	5	56	theme	cell	740:743	arg1	viability					745:753	cell viability	740:753	cell viability	740:753	Following extrusion, approximately 70%-75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs.
28691691	1	57	theme	hydrogels	123:131	arg1	printing					100:107	Three-dimensional printing	82:107	Three-dimensional printing of cell-laden hydrogels	82:131	Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs.
28691691	9	58	theme	endothelial	1161:1171	arg1	factor					1180:1185	vascular endothelial growth factor	1152:1185	vascular endothelial growth factor	1152:1185	Two model proteins, bovine serum albumin and vascular endothelial growth factor were loaded into the bioink.
28691691	1	59	theme	tissue-engineered	213:229	arg1	constructs					231:240	patient-specific or complex tissue-engineered constructs	185:240	patient-specific or complex tissue-engineered constructs	185:240	Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs.
28691691	11	60	theme	agents	1590:1595	arg1	release					1559:1565	controlled release	1548:1565	controlled release of biologically active agents such as growth factors	1548:1618	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	6	61	theme	Mechanical	814:823	arg1	properties					825:834	Mechanical properties	814:834	Mechanical properties of scaffolds comprised of the composite bioink	814:881	Mechanical properties of scaffolds comprised of the composite bioink decreased over time when stored under cell culture conditions.
28691691	7	62	theme	constructs	981:990	arg1	shape					960:964	shape	960:964	shape of the plotted constructs	960:990	Nevertheless, shape of the plotted constructs was preserved even over longer cultivation periods.
28691691	2	63	theme	shape	312:316	arg1	fidelity					318:325	high shape fidelity	307:325	high shape fidelity	307:325	However, it is still challenging to print structures with both, high shape fidelity and cell vitality.
28691691	3	64	theme	extrusion-based	446:460	arg1	plotting					472:479	the extrusion-based method 3D plotting	442:479	the extrusion-based method 3D plotting	442:479	Herein, we used a synthetic nanosilicate clay, called Laponite, to build up scaffolds utilising the extrusion-based method 3D plotting.
28691691	9	65	theme	vascular	1152:1159	arg1	factor					1180:1185	vascular endothelial growth factor	1152:1185	vascular endothelial growth factor	1152:1185	Two model proteins, bovine serum albumin and vascular endothelial growth factor were loaded into the bioink.
28691691	2	66	with	structures	285:294	arg1	fidelity					318:325	high shape fidelity	307:325	high shape fidelity	307:325	However, it is still challenging to print structures with both, high shape fidelity and cell vitality.
28691691	2	66	with	structures	285:294	arg1	vitality					336:343	cell vitality	331:343	cell vitality	331:343	However, it is still challenging to print structures with both, high shape fidelity and cell vitality.
28691691	0	67	theme	3D	39:40	arg1	printing					47:54	3D cell printing	39:54	3D cell printing for skeletal application	39:79	Development of a clay based bioink for 3D cell printing for skeletal application.
28691691	1	68	from	approach	160:167	arg1	route					176:180	the route	172:180	the route	172:180	Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs.
28691691	11	69	theme	growth	1605:1610	arg1	factors					1612:1618	growth factors	1605:1618	growth factors	1605:1618	In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
28691691	10	70	theme	factors	1263:1269	arg1	release					1240:1246	the release	1236:1246	the release of both growth factors	1236:1269	We demonstrate that the release of both growth factors significantly changed to a more sustained profile by inclusion of Laponite in comparison to an alginate-methylcellulose blend in the absence of Laponite.
27209574	3	0	theme	high	742:745	arg1	0.29±0.13cm					775:785	0.29±0.13cm(2)	775:788	0.29±0.13cm(2)	775:788	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	3	0	theme	high	742:745	arg1	area					769:772	electroactive surface area	747:772	an extremely high electroactive surface area (0.29±0.13cm(2))	729:789	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	1	1	theme	fractal	156:162	arg1	nanocauliflower					173:187	fractal platinum nanocauliflower	156:187	fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7)	156:317	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	7	2	theme	nanocellulose-graphene-nanoplatinum	1397:1431	arg1	platform					1470:1477	an excellent paper-based platform	1445:1477	an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing	1445:1600	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	7	2	theme	nanocellulose-graphene-nanoplatinum	1397:1431	arg1	material					1433:1440	the nanocellulose-graphene-nanoplatinum material	1393:1440	the nanocellulose-graphene-nanoplatinum material	1393:1440	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	7	3	theme	whole	1554:1558	arg1	cells					1560:1564	whole cells	1554:1564	whole cells	1554:1564	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	0	4	theme	biosensing	74:83	arg1	point					60:64	point	60:64	point of care biosensing	60:83	A paper based graphene-nanocauliflower hybrid composite for point of care biosensing.
27209574	1	5	dep	bacteria	283:290	arg1	O157					310:313	Escherichia coli O157	293:313	Escherichia coli O157:H7	293:316	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	6	6	theme	response	1240:1247	arg1	similar					1303:1309	similar	1303:1309	similar	1303:1309	The response time (6s for glucose and 12min for E. coli) were also similar to silicon biochip and commercial electrode sensors.
27209574	6	6	theme	response	1240:1247	arg1	time					1249:1252	The response time	1236:1252	The response time (6s for glucose and 12min for E. coli)	1236:1291	The response time (6s for glucose and 12min for E. coli) were also similar to silicon biochip and commercial electrode sensors.
27209574	1	7	theme	platinum	164:171	arg1	nanocauliflower					173:187	fractal platinum nanocauliflower	156:187	fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7)	156:317	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	5	8	theme	biosensing	1213:1222	arg1	literature					1224:1233	the biosensing literature	1209:1233	the biosensing literature	1209:1233	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	7	9	theme	cells	1560:1564	arg1	development					1483:1493	development	1483:1493	development of electrochemical biosensors targeting small molecules or whole cells	1483:1564	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	2	10	theme	ascorbic	542:549	arg1	treatment					531:539	chemical treatment	522:539	chemical treatment (ascorbic acid)	522:555	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	2	10	theme	ascorbic	542:549	arg1	acid					551:554	ascorbic acid	542:554	ascorbic acid	542:554	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	5	11	theme	≈4	1125:1126	arg1	-1					1134:1135	-1	1134:1135	-1	1134:1135	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	5	11	theme	≈4	1125:1126	arg1	CFUmL					1128:1132	≈4 CFUmL	1125:1132	≈4 CFUmL(-1)	1125:1136	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	2	12	theme	scanning	340:347	arg1	microscopy					358:367	scanning electron microscopy	340:367	scanning electron microscopy	340:367	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	3	13	theme	impedance	844:852	arg1	spectroscopy					854:865	electrochemical impedance spectroscopy	828:865	electrochemical impedance spectroscopy	828:865	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	7	14	theme	biosensors	1514:1523	arg1	development					1483:1493	development	1483:1493	development of electrochemical biosensors targeting small molecules or whole cells	1483:1564	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	3	15	theme	Fractal	558:564	arg1	nanoplatinum					566:577	Fractal nanoplatinum	558:577	Fractal nanoplatinum with cauliflower-like morphology	558:610	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	2	16	theme	electron	349:356	arg1	microscopy					358:367	scanning electron microscopy	340:367	scanning electron microscopy	340:367	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	1	17	theme	pathogenic	272:281	arg1	bacteria					283:290	pathogenic bacteria	272:290	pathogenic bacteria (Escherichia coli O157:H7)	272:317	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	6	18	dep	time	1249:1252	arg1	6s					1255:1256	6s	1255:1256	6s for glucose and 12min for E. coli	1255:1290	The response time (6s for glucose and 12min for E. coli) were also similar to silicon biochip and commercial electrode sensors.
27209574	5	19	theme	E.	1108:1109	arg1	O157					1116:1119	O157	1116:1119	E. coli O157:H7 (≈4 CFUmL(-1))	1108:1137	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	2	20	theme	chemical	522:529	arg1	treatment					531:539	chemical treatment	522:539	chemical treatment (ascorbic acid)	522:555	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	2	20	theme	chemical	522:529	arg1	acid					551:554	ascorbic acid	542:554	ascorbic acid	542:554	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	1	21	dep	O157	310:313	arg1	H7					315:316	H7	315:316	Escherichia coli O157:H7	293:316	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	4	22	theme	platinum	872:879	arg1	point					1028:1032	a point	1026:1032	a point of care biosensor	1026:1050	The platinum surface was functionalized with either glucose oxidase (via chitosan encapsulation) or a RNA aptamer (via covalent linking) for demonstration as a point of care biosensor.
27209574	4	22	theme	platinum	872:879	arg1	surface					881:887	The platinum surface	868:887	The platinum surface	868:887	The platinum surface was functionalized with either glucose oxidase (via chitosan encapsulation) or a RNA aptamer (via covalent linking) for demonstration as a point of care biosensor.
27209574	3	23	theme	electrochemical	828:842	arg1	spectroscopy					854:865	electrochemical impedance spectroscopy	828:865	electrochemical impedance spectroscopy	828:865	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	3	24	with	nanoplatinum	566:577	arg1	morphology					601:610	cauliflower-like morphology	584:610	cauliflower-like morphology	584:610	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	3	25	theme	surface	761:767	arg1	0.29±0.13cm					775:785	0.29±0.13cm(2)	775:788	0.29±0.13cm(2)	775:788	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	3	25	theme	surface	761:767	arg1	area					769:772	electroactive surface area	747:772	an extremely high electroactive surface area (0.29±0.13cm(2))	729:789	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	7	26	theme	excellent	1448:1456	arg1	platform					1470:1477	an excellent paper-based platform	1445:1477	an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing	1445:1600	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	7	26	theme	excellent	1448:1456	arg1	material					1433:1440	the nanocellulose-graphene-nanoplatinum material	1393:1440	the nanocellulose-graphene-nanoplatinum material	1393:1440	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	3	27	theme	cauliflower-like	584:599	arg1	morphology					601:610	cauliflower-like morphology	584:610	cauliflower-like morphology	584:610	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	7	28	from	use	1570:1572	arg1	point					1577:1581	point	1577:1581	point of care biosensing	1577:1600	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	2	29	theme	dispersive	380:389	arg1	spectroscopy					391:402	energy dispersive spectroscopy	373:402	energy dispersive spectroscopy	373:402	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	4	30	theme	care	1037:1040	arg1	biosensor					1042:1050	care biosensor	1037:1050	care biosensor	1037:1050	The platinum surface was functionalized with either glucose oxidase (via chitosan encapsulation) or a RNA aptamer (via covalent linking) for demonstration as a point of care biosensor.
27209574	1	31	theme	bacteria	283:290	arg1	use					193:195	use	193:195	use in electrochemical biosensing of small molecules (glucose)	193:254	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	1	31	theme	bacteria	283:290	arg1	detection					259:267	detection	259:267	detection of pathogenic bacteria (Escherichia coli O157:H7)	259:317	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	7	32	theme	paper-based	1458:1468	arg1	platform					1470:1477	an excellent paper-based platform	1445:1477	an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing	1445:1600	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	7	32	theme	paper-based	1458:1468	arg1	material					1433:1440	the nanocellulose-graphene-nanoplatinum material	1393:1440	the nanocellulose-graphene-nanoplatinum material	1393:1440	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	5	33	theme	detection	1057:1065	arg1	competitive					1144:1154	competitive	1144:1154	competitive	1144:1154	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	5	33	theme	detection	1057:1065	arg1	limit					1067:1071	The detection limit	1053:1071	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1))	1053:1137	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	2	34	theme	energy	373:378	arg1	spectroscopy					391:402	energy dispersive spectroscopy	373:402	energy dispersive spectroscopy	373:402	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	0	35	theme	graphene-nanocauliflower	14:37	arg1	composite					46:54	graphene-nanocauliflower hybrid composite	14:54	graphene-nanocauliflower hybrid composite	14:54	A paper based graphene-nanocauliflower hybrid composite for point of care biosensing.
27209574	1	36	theme	electrochemical	200:214	arg1	biosensing					216:225	electrochemical biosensing	200:225	electrochemical biosensing of small molecules (glucose)	200:254	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	1	37	theme	Escherichia	293:303	arg1	O157					310:313	Escherichia coli O157	293:313	Escherichia coli O157:H7	293:316	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	6	38	theme	electrode	1345:1353	arg1	sensors					1355:1361	commercial electrode sensors	1334:1361	commercial electrode sensors	1334:1361	The response time (6s for glucose and 12min for E. coli) were also similar to silicon biochip and commercial electrode sensors.
27209574	1	39	theme	first	105:109	arg1	report					111:116	the first report	101:116	the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7)	101:317	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	2	40	theme	oxide-coated	423:434	arg1	nanocellulose					436:448	graphene oxide-coated nanocellulose	414:448	graphene oxide-coated nanocellulose	414:448	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	5	41	dep	H7	1121:1122	arg1	-1					1134:1135	-1	1134:1135	-1	1134:1135	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	5	41	dep	H7	1121:1122	arg1	CFUmL					1128:1132	≈4 CFUmL	1125:1132	≈4 CFUmL(-1)	1125:1136	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	4	42	theme	RNA	970:972	arg1	aptamer					974:980	a RNA aptamer	968:980	a RNA aptamer (via covalent linking)	968:1003	The platinum surface was functionalized with either glucose oxidase (via chitosan encapsulation) or a RNA aptamer (via covalent linking) for demonstration as a point of care biosensor.
27209574	7	43	theme	small	1535:1539	arg1	molecules					1541:1549	small molecules	1535:1549	small molecules	1535:1549	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	2	44	theme	graphene	414:421	arg1	nanocellulose					436:448	graphene oxide-coated nanocellulose	414:448	graphene oxide-coated nanocellulose	414:448	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	0	45	theme	hybrid	39:44	arg1	composite					46:54	graphene-nanocauliflower hybrid composite	14:54	graphene-nanocauliflower hybrid composite	14:54	A paper based graphene-nanocauliflower hybrid composite for point of care biosensing.
27209574	7	46	theme	biosensing	1591:1600	arg1	point					1577:1581	point	1577:1581	point of care biosensing	1577:1600	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	1	47	from	detection	259:267	arg1	biosensing					216:225	electrochemical biosensing	200:225	electrochemical biosensing of small molecules (glucose)	200:254	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	7	48	theme	electrochemical	1498:1512	arg1	biosensors					1514:1523	electrochemical biosensors	1498:1523	electrochemical biosensors targeting small molecules	1498:1549	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	5	49	theme	reported	1189:1196	arg1	devices					1198:1204	, previously reported devices	1176:1204	devices	1198:1204	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	1	50	theme	small	230:234	arg1	glucose					247:253	glucose	247:253	glucose	247:253	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	1	50	theme	small	230:234	arg1	molecules					236:244	small molecules	230:244	small molecules (glucose)	230:254	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	2	51	theme	thermal	480:486	arg1	treatment					488:496	both thermal treatment	475:496	both thermal treatment	475:496	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	5	52	dep	O157	1116:1119	arg1	coli					1111:1114	E. coli O157:H7 (≈4 CFUmL(-1))	1108:1137	E. coli O157:H7 (≈4 CFUmL(-1))	1108:1137	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	5	52	dep	O157	1116:1119	arg1	H7					1121:1122	H7	1121:1122	E. coli O157:H7 (≈4 CFUmL(-1))	1108:1137	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	3	53	theme	pulsed	665:670	arg1	sonoelectrodeposition					672:692	pulsed sonoelectrodeposition	665:692	pulsed sonoelectrodeposition	665:692	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	3	54	theme	electroactive	747:759	arg1	0.29±0.13cm					775:785	0.29±0.13cm(2)	775:788	0.29±0.13cm(2)	775:788	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	3	54	theme	electroactive	747:759	arg1	area					769:772	electroactive surface area	747:772	an extremely high electroactive surface area (0.29±0.13cm(2))	729:789	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	6	55	theme	commercial	1334:1343	arg1	sensors					1355:1361	commercial electrode sensors	1334:1361	commercial electrode sensors	1334:1361	The response time (6s for glucose and 12min for E. coli) were also similar to silicon biochip and commercial electrode sensors.
27209574	5	56	from	devices	1198:1204	arg1	literature					1224:1233	the biosensing literature	1209:1233	the biosensing literature	1209:1233	The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature.
27209574	3	57	theme	conductive	707:716	arg1	paper					718:722	a conductive paper	705:722	a conductive paper	705:722	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	7	58	theme	care	1586:1589	arg1	biosensing					1591:1600	care biosensing	1586:1600	care biosensing	1586:1600	The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.
27209574	3	59	theme	oxide	647:651	arg1	paper					653:657	the reduced graphene oxide paper	626:657	the reduced graphene oxide paper using pulsed sonoelectrodeposition	626:692	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	1	60	theme	graphene	121:128	arg1	paper					130:134	graphene paper	121:134	graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7)	121:317	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	1	61	theme	molecules	236:244	arg1	biosensing					216:225	electrochemical biosensing	200:225	electrochemical biosensing of small molecules (glucose)	200:254	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	4	62	theme	chitosan	941:948	arg1	encapsulation					950:962	chitosan encapsulation	941:962	chitosan encapsulation	941:962	The platinum surface was functionalized with either glucose oxidase (via chitosan encapsulation) or a RNA aptamer (via covalent linking) for demonstration as a point of care biosensor.
27209574	1	63	from	use	193:195	arg1	biosensing					216:225	electrochemical biosensing	200:225	electrochemical biosensing of small molecules (glucose)	200:254	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	1	64	theme	paper	130:134	arg1	report					111:116	the first report	101:116	the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7)	101:317	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	4	65	theme	glucose	920:926	arg1	oxidase					928:934	glucose oxidase	920:934	glucose oxidase (via chitosan encapsulation)	920:963	The platinum surface was functionalized with either glucose oxidase (via chitosan encapsulation) or a RNA aptamer (via covalent linking) for demonstration as a point of care biosensor.
27209574	2	66	theme	Raman	320:324	arg1	spectroscopy					326:337	Raman spectroscopy	320:337	Raman spectroscopy	320:337	Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid).
27209574	0	67	theme	care	69:72	arg1	biosensing					74:83	care biosensing	69:83	care biosensing	69:83	A paper based graphene-nanocauliflower hybrid composite for point of care biosensing.
27209574	3	68	theme	reduced	630:636	arg1	paper					653:657	the reduced graphene oxide paper	626:657	the reduced graphene oxide paper using pulsed sonoelectrodeposition	626:692	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	3	69	theme	cyclic	805:810	arg1	voltammetry					812:822	cyclic voltammetry	805:822	cyclic voltammetry	805:822	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	6	70	theme	silicon	1314:1320	arg1	biochip					1322:1328	silicon biochip	1314:1328	silicon biochip	1314:1328	The response time (6s for glucose and 12min for E. coli) were also similar to silicon biochip and commercial electrode sensors.
27209574	1	71	dep	Escherichia	293:303	arg1	coli					305:308	coli	305:308	coli	305:308	We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7).
27209574	3	72	theme	graphene	638:645	arg1	paper					653:657	the reduced graphene oxide paper	626:657	the reduced graphene oxide paper using pulsed sonoelectrodeposition	626:692	Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy.
27209574	4	73	theme	biosensor	1042:1050	arg1	point					1028:1032	a point	1026:1032	a point of care biosensor	1026:1050	The platinum surface was functionalized with either glucose oxidase (via chitosan encapsulation) or a RNA aptamer (via covalent linking) for demonstration as a point of care biosensor.
27209574	4	73	theme	biosensor	1042:1050	arg1	surface					881:887	The platinum surface	868:887	The platinum surface	868:887	The platinum surface was functionalized with either glucose oxidase (via chitosan encapsulation) or a RNA aptamer (via covalent linking) for demonstration as a point of care biosensor.
27282535	4	0	theme	process	878:884	arg1	history					863:869	thermal history	855:869	thermal history	855:869	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	4	0	theme	process	878:884	arg1	composition					839:849	composition	839:849	composition	839:849	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	2	1	theme	dried	365:369	arg1	solids					371:376	dried solids	365:376	dried solids	365:376	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	5	2	from	transition	1004:1013	arg1	form					991:994	the metastable form	976:994	the metastable form	976:994	Heating of some inositol-rich amorphous solids showed crystallization of myo-inositol in the metastable form and its transition to stable form before melting.
27282535	1	3	theme	subsequent	247:256	arg1	storage					258:264	freeze-drying and subsequent storage	229:264	freeze-drying and subsequent storage	229:264	The purpose of this study was to elucidate how co-solutes affect the crystallization of small solute molecules during freeze-drying and subsequent storage.
27282535	0	4	theme	crystal	68:74	arg1	form					76:79	the crystal form	64:79	the crystal form of freeze-dried myo-inositol	64:108	Effect of co-solutes and process variables on crystallinity and the crystal form of freeze-dried myo-inositol.
27282535	8	5	dep	solute	1335:1340	arg1	composition					1342:1352	composition	1342:1352	composition	1342:1352	Optimizing solute composition and processes should be a potent way to control crystal form and crystallinity of components in freeze-dried formulations.
27282535	5	6	theme	inositol-rich	903:915	arg1	solids					927:932	some inositol-rich amorphous solids	898:932	some inositol-rich amorphous solids	898:932	Heating of some inositol-rich amorphous solids showed crystallization of myo-inositol in the metastable form and its transition to stable form before melting.
27282535	4	7	theme	crystal	790:796	arg1	forms					798:802	crystal forms	790:802	crystal forms	790:802	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	8	8	theme	components	1436:1445	arg1	form					1410:1413	crystal form	1402:1413	crystal form	1402:1413	Optimizing solute composition and processes should be a potent way to control crystal form and crystallinity of components in freeze-dried formulations.
27282535	8	8	theme	components	1436:1445	arg1	crystallinity					1419:1431	crystallinity	1419:1431	crystallinity	1419:1431	Optimizing solute composition and processes should be a potent way to control crystal form and crystallinity of components in freeze-dried formulations.
27282535	5	9	theme	myo-inositol	960:971	arg1	crystallization					941:955	crystallization	941:955	crystallization of myo-inositol in the metastable form	941:994	Heating of some inositol-rich amorphous solids showed crystallization of myo-inositol in the metastable form and its transition to stable form before melting.
27282535	5	9	theme	myo-inositol	960:971	arg1	transition					1004:1013	its transition	1000:1013	its transition to stable form	1000:1028	Heating of some inositol-rich amorphous solids showed crystallization of myo-inositol in the metastable form and its transition to stable form before melting.
27282535	0	10	theme	freeze-dried	84:95	arg1	myo-inositol					97:108	freeze-dried myo-inositol	84:108	freeze-dried myo-inositol	84:108	Effect of co-solutes and process variables on crystallinity and the crystal form of freeze-dried myo-inositol.
27282535	0	11	from	Effect	0:5	arg1	form					76:79	the crystal form	64:79	the crystal form of freeze-dried myo-inositol	64:108	Effect of co-solutes and process variables on crystallinity and the crystal form of freeze-dried myo-inositol.
27282535	0	11	from	Effect	0:5	arg1	crystallinity					46:58	crystallinity	46:58	crystallinity	46:58	Effect of co-solutes and process variables on crystallinity and the crystal form of freeze-dried myo-inositol.
27282535	2	12	from	40k	337:339	arg1	solutions					351:359	frozen solutions	344:359	frozen solutions	344:359	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	2	12	from	40k	337:339	arg1	solids					371:376	dried solids	365:376	dried solids	365:376	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	8	13	theme	crystal	1402:1408	arg1	form					1410:1413	crystal form	1402:1413	crystal form	1402:1413	Optimizing solute composition and processes should be a potent way to control crystal form and crystallinity of components in freeze-dried formulations.
27282535	5	14	theme	stable	1018:1023	arg1	form					1025:1028	stable form	1018:1028	stable form	1018:1028	Heating of some inositol-rich amorphous solids showed crystallization of myo-inositol in the metastable form and its transition to stable form before melting.
27282535	8	15	from	components	1436:1445	arg1	formulations					1463:1474	freeze-dried formulations	1450:1474	freeze-dried formulations	1450:1474	Optimizing solute composition and processes should be a potent way to control crystal form and crystallinity of components in freeze-dried formulations.
27282535	4	16	dep	myo-inositol	777:788	arg1	forms					798:802	crystal forms	790:802	crystal forms	790:802	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	3	17	theme	non-crystalline	546:560	arg1	state					570:574	the non-crystalline mixture state	542:574	the non-crystalline mixture state	542:574	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	7	18	theme	dextran-rich	1293:1304	arg1	solids					1316:1321	dextran-rich amorphous solids	1293:1321	dextran-rich amorphous solids	1293:1321	Sufficient direct molecular interactions (e.g., hydrogen bonding) should explain the stability of dextran-rich amorphous solids.
27282535	2	19	theme	myo-inositol	295:306	arg1	mixture					316:322	its mixture	312:322	its mixture with dextran 40k in frozen solutions and dried solids	312:376	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	2	19	theme	myo-inositol	295:306	arg1	profiles					283:290	Crystallization profiles	267:290	Crystallization profiles of myo-inositol	267:306	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	5	20	theme	solids	927:932	arg1	Heating					887:893	Heating	887:893	Heating of some inositol-rich amorphous solids	887:932	Heating of some inositol-rich amorphous solids showed crystallization of myo-inositol in the metastable form and its transition to stable form before melting.
27282535	4	21	theme	thermal	855:861	arg1	history					863:869	thermal history	855:869	thermal history	855:869	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	6	22	theme	solutions	1085:1093	arg1	Heat-treatment					1046:1059	Heat-treatment	1046:1059	Heat-treatment of inositol-rich frozen solutions	1046:1093	Heat-treatment of inositol-rich frozen solutions resulted in high crystallinity stable-form inositol solids, leaving dextran in the amorphous state.
27282535	4	23	theme	myo-inositol	777:788	arg1	variety					766:772	a variety	764:772	a variety of myo-inositol crystal forms and crystallinity	764:820	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	4	23	theme	myo-inositol	777:788	arg1	crystallinity					808:820	crystallinity	808:820	crystallinity	808:820	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	4	23	theme	myo-inositol	777:788	arg1	myo-inositol					777:788	myo-inositol crystal forms and crystallinity	777:820	myo-inositol	777:788	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	6	24	theme	stable-form	1126:1136	arg1	solids					1147:1152	high crystallinity stable-form inositol solids	1107:1152	high crystallinity stable-form inositol solids	1107:1152	Heat-treatment of inositol-rich frozen solutions resulted in high crystallinity stable-form inositol solids, leaving dextran in the amorphous state.
27282535	0	25	theme	myo-inositol	97:108	arg1	form					76:79	the crystal form	64:79	the crystal form of freeze-dried myo-inositol	64:108	Effect of co-solutes and process variables on crystallinity and the crystal form of freeze-dried myo-inositol.
27282535	0	25	theme	myo-inositol	97:108	arg1	crystallinity					46:58	crystallinity	46:58	crystallinity	46:58	Effect of co-solutes and process variables on crystallinity and the crystal form of freeze-dried myo-inositol.
27282535	5	26	theme	amorphous	917:925	arg1	solids					927:932	some inositol-rich amorphous solids	898:932	some inositol-rich amorphous solids	898:932	Heating of some inositol-rich amorphous solids showed crystallization of myo-inositol in the metastable form and its transition to stable form before melting.
27282535	2	27	with	mixture	316:322	arg1	40k					337:339	dextran 40k	329:339	dextran 40k in frozen solutions and dried solids	329:376	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	5	28	from	crystallization	941:955	arg1	form					991:994	the metastable form	976:994	the metastable form	976:994	Heating of some inositol-rich amorphous solids showed crystallization of myo-inositol in the metastable form and its transition to stable form before melting.
27282535	4	29	theme	lower	706:710	arg1	ratio					717:721	a lower mass ratio	704:721	a lower mass ratio of dextran	704:732	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	6	30	theme	inositol	1138:1145	arg1	solids					1147:1152	high crystallinity stable-form inositol solids	1107:1152	high crystallinity stable-form inositol solids	1107:1152	Heat-treatment of inositol-rich frozen solutions resulted in high crystallinity stable-form inositol solids, leaving dextran in the amorphous state.
27282535	2	31	theme	thermal	395:401	arg1	DSC					413:415	DSC	413:415	DSC	413:415	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	2	31	theme	thermal	395:401	arg1	analysis					403:410	thermal analysis	395:410	thermal analysis (DSC)	395:416	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	4	32	with	Co-lyophilization	681:697	arg1	ratio					717:721	a lower mass ratio	704:721	a lower mass ratio of dextran	704:732	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	2	33	theme	dextran	329:335	arg1	40k					337:339	dextran 40k	329:339	dextran 40k in frozen solutions and dried solids	329:376	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	6	34	theme	high	1107:1110	arg1	solids					1147:1152	high crystallinity stable-form inositol solids	1107:1152	high crystallinity stable-form inositol solids	1107:1152	Heat-treatment of inositol-rich frozen solutions resulted in high crystallinity stable-form inositol solids, leaving dextran in the amorphous state.
27282535	7	35	theme	Sufficient	1195:1204	arg1	interactions					1223:1234	Sufficient direct molecular interactions	1195:1234	Sufficient direct molecular interactions (e.g., hydrogen bonding)	1195:1259	Sufficient direct molecular interactions (e.g., hydrogen bonding) should explain the stability of dextran-rich amorphous solids.
27282535	7	36	theme	molecular	1213:1221	arg1	interactions					1223:1234	Sufficient direct molecular interactions	1195:1234	Sufficient direct molecular interactions (e.g., hydrogen bonding)	1195:1259	Sufficient direct molecular interactions (e.g., hydrogen bonding) should explain the stability of dextran-rich amorphous solids.
27282535	4	37	contain	containing	753:762	arg1	solids					746:751	solids	746:751	solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process	746:884	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	4	37	contain	containing	753:762	arg2	myo-inositol					777:788	myo-inositol crystal forms and crystallinity	777:820	myo-inositol	777:788	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	4	37	contain	containing	753:762	arg2	variety					766:772	a variety	764:772	a variety of myo-inositol crystal forms and crystallinity	764:820	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	4	37	contain	containing	753:762	arg2	crystallinity					808:820	crystallinity	808:820	crystallinity	808:820	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	4	38	theme	crystallinity	808:820	arg1	variety					766:772	a variety	764:772	a variety of myo-inositol crystal forms and crystallinity	764:820	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	4	38	theme	crystallinity	808:820	arg1	crystallinity					808:820	crystallinity	808:820	crystallinity	808:820	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	4	38	theme	crystallinity	808:820	arg1	myo-inositol					777:788	myo-inositol crystal forms and crystallinity	777:820	myo-inositol	777:788	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	7	39	theme	amorphous	1306:1314	arg1	solids					1316:1321	dextran-rich amorphous solids	1293:1321	dextran-rich amorphous solids	1293:1321	Sufficient direct molecular interactions (e.g., hydrogen bonding) should explain the stability of dextran-rich amorphous solids.
27282535	3	40	theme	mixture	562:568	arg1	state					570:574	the non-crystalline mixture state	542:574	the non-crystalline mixture state	542:574	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	4	41	dep	composition	839:849	arg1	the					835:837	the	835:837	the	835:837	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	2	42	theme	powder-X-ray	419:430	arg1	diffraction					432:442	powder-X-ray diffraction	419:442	powder-X-ray diffraction	419:442	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	3	43	theme	dried	644:648	arg1	solids					650:655	dried solids	644:655	dried solids	644:655	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	5	44	theme	metastable	980:989	arg1	form					991:994	the metastable form	976:994	the metastable form	976:994	Heating of some inositol-rich amorphous solids showed crystallization of myo-inositol in the metastable form and its transition to stable form before melting.
27282535	3	45	theme	dextran	507:513	arg1	ratios					497:502	Higher mass ratios	485:502	Higher mass ratios of dextran	485:513	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	2	46	theme	PXRD	470:473	arg1	analysis					475:482	simultaneous DSC and PXRD analysis	449:482	analysis	475:482	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	3	47	theme	mass	492:495	arg1	ratios					497:502	Higher mass ratios	485:502	Higher mass ratios of dextran	485:513	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	8	48	from	crystallinity	1419:1431	arg1	formulations					1463:1474	freeze-dried formulations	1450:1474	freeze-dried formulations	1450:1474	Optimizing solute composition and processes should be a potent way to control crystal form and crystallinity of components in freeze-dried formulations.
27282535	3	49	theme	solids	650:655	arg1	process					619:625	freeze-drying process	605:625	freeze-drying process	605:625	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	3	49	theme	solids	650:655	arg1	exposure					632:639	exposure	632:639	exposure of dried solids to higher temperatures	632:678	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	8	50	theme	potent	1380:1385	arg1	way					1387:1389	a potent way	1378:1389	a potent way to control crystal form and crystallinity of components in freeze-dried formulations	1378:1474	Optimizing solute composition and processes should be a potent way to control crystal form and crystallinity of components in freeze-dried formulations.
27282535	1	51	theme	small	199:203	arg1	molecules					212:220	small solute molecules	199:220	small solute molecules	199:220	The purpose of this study was to elucidate how co-solutes affect the crystallization of small solute molecules during freeze-drying and subsequent storage.
27282535	0	52	theme	process	25:31	arg1	Effect					0:5	Effect	0:5	Effect of co-solutes and process variables on crystallinity and the crystal form of freeze-dried myo-inositol.	0:109	Effect of co-solutes and process variables on crystallinity and the crystal form of freeze-dried myo-inositol.
27282535	7	53	theme	direct	1206:1211	arg1	interactions					1223:1234	Sufficient direct molecular interactions	1195:1234	Sufficient direct molecular interactions (e.g., hydrogen bonding)	1195:1259	Sufficient direct molecular interactions (e.g., hydrogen bonding) should explain the stability of dextran-rich amorphous solids.
27282535	8	54	theme	freeze-dried	1450:1461	arg1	formulations					1463:1474	freeze-dried formulations	1450:1474	freeze-dried formulations	1450:1474	Optimizing solute composition and processes should be a potent way to control crystal form and crystallinity of components in freeze-dried formulations.
27282535	1	55	theme	solute	205:210	arg1	molecules					212:220	small solute molecules	199:220	small solute molecules	199:220	The purpose of this study was to elucidate how co-solutes affect the crystallization of small solute molecules during freeze-drying and subsequent storage.
27282535	6	56	theme	inositol-rich	1064:1076	arg1	solutions					1085:1093	inositol-rich frozen solutions	1064:1093	inositol-rich frozen solutions	1064:1093	Heat-treatment of inositol-rich frozen solutions resulted in high crystallinity stable-form inositol solids, leaving dextran in the amorphous state.
27282535	3	57	theme	frozen	580:585	arg1	solutions					587:595	frozen solutions	580:595	frozen solutions	580:595	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	6	58	from	dextran	1163:1169	arg1	state					1188:1192	the amorphous state	1174:1192	the amorphous state	1174:1192	Heat-treatment of inositol-rich frozen solutions resulted in high crystallinity stable-form inositol solids, leaving dextran in the amorphous state.
27282535	3	59	theme	Higher	485:490	arg1	ratios					497:502	Higher mass ratios	485:502	Higher mass ratios of dextran	485:513	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	2	60	theme	simultaneous	449:460	arg1	DSC					462:464	simultaneous DSC and PXRD analysis	449:482	DSC	462:464	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	7	61	theme	hydrogen	1243:1250	arg1	bonding					1252:1258	hydrogen bonding	1243:1258	hydrogen bonding	1243:1258	Sufficient direct molecular interactions (e.g., hydrogen bonding) should explain the stability of dextran-rich amorphous solids.
27282535	2	62	theme	frozen	344:349	arg1	solutions					351:359	frozen solutions	344:359	frozen solutions	344:359	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	7	63	dep	bonding	1252:1258	arg1	e.g.					1237:1240	e.g.	1237:1240	e.g.	1237:1240	Sufficient direct molecular interactions (e.g., hydrogen bonding) should explain the stability of dextran-rich amorphous solids.
27282535	2	64	theme	Crystallization	267:281	arg1	profiles					283:290	Crystallization profiles	267:290	Crystallization profiles of myo-inositol	267:306	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	4	65	theme	dextran	726:732	arg1	ratio					717:721	a lower mass ratio	704:721	a lower mass ratio of dextran	704:732	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27282535	8	66	from	form	1410:1413	arg1	formulations					1463:1474	freeze-dried formulations	1450:1474	freeze-dried formulations	1450:1474	Optimizing solute composition and processes should be a potent way to control crystal form and crystallinity of components in freeze-dried formulations.
27282535	1	67	theme	molecules	212:220	arg1	crystallization					180:194	the crystallization	176:194	the crystallization of small solute molecules	176:220	The purpose of this study was to elucidate how co-solutes affect the crystallization of small solute molecules during freeze-drying and subsequent storage.
27282535	0	68	dep	co-solutes	10:19	arg1	variables					33:41	variables	33:41	variables	33:41	Effect of co-solutes and process variables on crystallinity and the crystal form of freeze-dried myo-inositol.
27282535	7	69	theme	solids	1316:1321	arg1	stability					1280:1288	the stability	1276:1288	the stability of dextran-rich amorphous solids	1276:1321	Sufficient direct molecular interactions (e.g., hydrogen bonding) should explain the stability of dextran-rich amorphous solids.
27282535	6	70	theme	amorphous	1178:1186	arg1	state					1188:1192	the amorphous state	1174:1192	the amorphous state	1174:1192	Heat-treatment of inositol-rich frozen solutions resulted in high crystallinity stable-form inositol solids, leaving dextran in the amorphous state.
27282535	2	71	with	profiles	283:290	arg1	40k					337:339	dextran 40k	329:339	dextran 40k in frozen solutions and dried solids	329:376	Crystallization profiles of myo-inositol and its mixture with dextran 40k in frozen solutions and dried solids were assessed by thermal analysis (DSC), powder-X-ray diffraction, and simultaneous DSC and PXRD analysis.
27282535	3	72	theme	higher	660:665	arg1	temperatures					667:678	higher temperatures	660:678	higher temperatures	660:678	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	3	73	theme	freeze-drying	605:617	arg1	process					619:625	freeze-drying process	605:625	freeze-drying process	605:625	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	1	74	theme	study	131:135	arg1	purpose					115:121	The purpose	111:121	The purpose of this study	111:135	The purpose of this study was to elucidate how co-solutes affect the crystallization of small solute molecules during freeze-drying and subsequent storage.
27282535	6	75	theme	crystallinity	1112:1124	arg1	solids					1147:1152	high crystallinity stable-form inositol solids	1107:1152	high crystallinity stable-form inositol solids	1107:1152	Heat-treatment of inositol-rich frozen solutions resulted in high crystallinity stable-form inositol solids, leaving dextran in the amorphous state.
27282535	1	76	theme	freeze-drying	229:241	arg1	storage					258:264	freeze-drying and subsequent storage	229:264	freeze-drying and subsequent storage	229:264	The purpose of this study was to elucidate how co-solutes affect the crystallization of small solute molecules during freeze-drying and subsequent storage.
27282535	0	77	theme	co-solutes	10:19	arg1	Effect					0:5	Effect	0:5	Effect of co-solutes and process variables on crystallinity and the crystal form of freeze-dried myo-inositol.	0:109	Effect of co-solutes and process variables on crystallinity and the crystal form of freeze-dried myo-inositol.
27282535	8	78	from	formulations	1463:1474	arg1	form					1410:1413	crystal form	1402:1413	crystal form	1402:1413	Optimizing solute composition and processes should be a potent way to control crystal form and crystallinity of components in freeze-dried formulations.
27282535	8	78	from	formulations	1463:1474	arg1	crystallinity					1419:1431	crystallinity	1419:1431	crystallinity	1419:1431	Optimizing solute composition and processes should be a potent way to control crystal form and crystallinity of components in freeze-dried formulations.
27282535	6	79	theme	frozen	1078:1083	arg1	solutions					1085:1093	inositol-rich frozen solutions	1064:1093	inositol-rich frozen solutions	1064:1093	Heat-treatment of inositol-rich frozen solutions resulted in high crystallinity stable-form inositol solids, leaving dextran in the amorphous state.
27282535	3	80	from	myo-inositol	526:537	arg1	state					570:574	the non-crystalline mixture state	542:574	the non-crystalline mixture state	542:574	Higher mass ratios of dextran maintained myo-inositol in the non-crystalline mixture state, in frozen solutions, during freeze-drying process, and exposure of dried solids to higher temperatures.
27282535	4	81	theme	mass	712:715	arg1	ratio					717:721	a lower mass ratio	704:721	a lower mass ratio of dextran	704:732	Co-lyophilization with a lower mass ratio of dextran resulted in solids containing a variety of myo-inositol crystal forms and crystallinity depending on the composition and thermal history of the process.
27288275	4	0	theme	phase	509:513	arg1	diagram					515:521	The phase diagram	505:521	The phase diagram	505:521	The phase diagram was determined using epifluorescence microscopy for vesicle populations with symmetric and asymmetric solution compositions across the membranes.
27288275	8	1	dep	solutions	1265:1273	arg1	presence					1223:1230	presence	1223:1230	presence	1223:1230	Moreover, we found that the miscibility temperatures markedly increased for vesicles in the presence of symmetric and asymmetric salt solutions.
27288275	8	1	dep	solutions	1265:1273	arg1	the					1219:1221	the	1219:1221	the	1219:1221	Moreover, we found that the miscibility temperatures markedly increased for vesicles in the presence of symmetric and asymmetric salt solutions.
27288275	4	2	theme	asymmetric	614:623	arg1	compositions					634:645	symmetric and asymmetric solution compositions	600:645	symmetric and asymmetric solution compositions across the membranes	600:666	The phase diagram was determined using epifluorescence microscopy for vesicle populations with symmetric and asymmetric solution compositions across the membranes.
27288275	0	3	theme	Membranes	78:86	arg1	Regions					59:65	Fluid-Fluid Coexistence Regions	35:65	Fluid-Fluid Coexistence Regions of Charged Membranes	35:86	Solution Asymmetry and Salt Expand Fluid-Fluid Coexistence Regions of Charged Membranes.
27288275	9	4	theme	charged	1377:1383	arg1	membranes					1385:1393	charged membranes	1377:1393	charged membranes	1377:1393	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	9	5	theme	solution	1333:1340	arg1	asymmetry					1342:1350	solution asymmetry	1333:1350	solution asymmetry	1333:1350	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	9	6	theme	membranes	1385:1393	arg1	behavior					1365:1372	the phase behavior	1355:1372	the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains	1355:1535	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	6	7	theme	buffer	857:862	arg1	presence					833:840	the presence	829:840	the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane)	829:929	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	9	8	theme	proteins	1500:1507	arg1	repartitioning					1482:1495	the repartitioning	1478:1495	the repartitioning of proteins within the membrane domains	1478:1535	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	2	9	theme	lipid	313:317	arg1	mixtures					319:326	ternary lipid mixtures	305:326	ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol	305:432	To mimic charged biological membranes, we studied phase separation and domain formation in GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol.
27288275	6	10	theme	high-saline	845:855	arg1	buffer					857:862	high-saline buffer	845:862	high-saline buffer (both symmetrically or asymmetrically present across the membrane)	845:929	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	4	11	theme	solution	625:632	arg1	compositions					634:645	symmetric and asymmetric solution compositions	600:645	symmetric and asymmetric solution compositions across the membranes	600:666	The phase diagram was determined using epifluorescence microscopy for vesicle populations with symmetric and asymmetric solution compositions across the membranes.
27288275	8	12	theme	salt	1260:1263	arg1	solutions					1265:1273	symmetric and asymmetric salt solutions	1235:1273	symmetric and asymmetric salt solutions	1235:1273	Moreover, we found that the miscibility temperatures markedly increased for vesicles in the presence of symmetric and asymmetric salt solutions.
27288275	2	13	theme	egg	340:342	arg1	sphingomyelin					344:356	egg sphingomyelin	340:356	egg sphingomyelin	340:356	To mimic charged biological membranes, we studied phase separation and domain formation in GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol.
27288275	5	14	theme	Trans-membrane	669:682	arg1	asymmetry					693:701	Trans-membrane solution asymmetry	669:701	Trans-membrane solution asymmetry	669:701	Trans-membrane solution asymmetry was found to affect the membrane phase state.
27288275	2	15	theme	domain	277:282	arg1	formation					284:292	domain formation	277:292	domain formation	277:292	To mimic charged biological membranes, we studied phase separation and domain formation in GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol.
27288275	2	16	theme	charged	391:397	arg1	dioleoylphosphatidylglycerol					405:432	the negatively charged lipid dioleoylphosphatidylglycerol	376:432	the negatively charged lipid dioleoylphosphatidylglycerol	376:432	To mimic charged biological membranes, we studied phase separation and domain formation in GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol.
27288275	8	17	theme	asymmetric	1249:1258	arg1	solutions					1265:1273	symmetric and asymmetric salt solutions	1235:1273	symmetric and asymmetric salt solutions	1235:1273	Moreover, we found that the miscibility temperatures markedly increased for vesicles in the presence of symmetric and asymmetric salt solutions.
27288275	5	18	theme	solution	684:691	arg1	asymmetry					693:701	Trans-membrane solution asymmetry	669:701	Trans-membrane solution asymmetry	669:701	Trans-membrane solution asymmetry was found to affect the membrane phase state.
27288275	9	19	theme	direct	1406:1411	arg1	implications					1413:1424	direct implications	1406:1424	direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains	1406:1535	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	1	20	theme	Liquid-liquid	89:101	arg1	separation					109:118	Liquid-liquid phase separation	89:118	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs)	89:155	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs) leads to the formation of intramembrane domains.
27288275	6	21	theme	coexistence	1024:1034	arg1	region					977:982	a significantly extended region	952:982	a significantly extended region of liquid-ordered and liquid-disordered coexistence	952:1034	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	0	22	theme	Solution	0:7	arg1	Asymmetry					9:17	Solution Asymmetry	0:17	Solution Asymmetry	0:17	Solution Asymmetry and Salt Expand Fluid-Fluid Coexistence Regions of Charged Membranes.
27288275	9	23	theme	substantial	1302:1312	arg1	effect					1314:1319	a substantial effect	1300:1319	a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains	1300:1535	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	1	24	theme	phase	103:107	arg1	separation					109:118	Liquid-liquid phase separation	89:118	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs)	89:155	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs) leads to the formation of intramembrane domains.
27288275	1	25	theme	intramembrane	183:195	arg1	domains					197:203	intramembrane domains	183:203	intramembrane domains	183:203	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs) leads to the formation of intramembrane domains.
27288275	4	26	theme	vesicle	575:581	arg1	populations					583:593	vesicle populations	575:593	vesicle populations with symmetric and asymmetric solution compositions across the membranes	575:666	The phase diagram was determined using epifluorescence microscopy for vesicle populations with symmetric and asymmetric solution compositions across the membranes.
27288275	9	27	contain	has	1402:1404	arg2	implications					1413:1424	direct implications	1406:1424	direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains	1406:1535	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	9	27	contain	has	1402:1404	arg1	membranes					1385:1393	charged membranes	1377:1393	charged membranes	1377:1393	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	1	28	theme	domains	197:203	arg1	formation					170:178	the formation	166:178	the formation of intramembrane domains	166:203	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs) leads to the formation of intramembrane domains.
27288275	2	29	theme	ternary	305:311	arg1	mixtures					319:326	ternary lipid mixtures	305:326	ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol	305:432	To mimic charged biological membranes, we studied phase separation and domain formation in GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol.
27288275	4	30	theme	symmetric	600:608	arg1	compositions					634:645	symmetric and asymmetric solution compositions	600:645	symmetric and asymmetric solution compositions across the membranes	600:666	The phase diagram was determined using epifluorescence microscopy for vesicle populations with symmetric and asymmetric solution compositions across the membranes.
27288275	4	31	with	populations	583:593	arg1	compositions					634:645	symmetric and asymmetric solution compositions	600:645	symmetric and asymmetric solution compositions across the membranes	600:666	The phase diagram was determined using epifluorescence microscopy for vesicle populations with symmetric and asymmetric solution compositions across the membranes.
27288275	9	32	theme	membrane	1520:1527	arg1	domains					1529:1535	the membrane domains	1516:1535	the membrane domains	1516:1535	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	6	33	from	diagram	818:824	arg1	presence					833:840	the presence	829:840	the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane)	829:929	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	0	34	theme	Fluid-Fluid	35:45	arg1	Regions					59:65	Fluid-Fluid Coexistence Regions	35:65	Fluid-Fluid Coexistence Regions of Charged Membranes	35:86	Solution Asymmetry and Salt Expand Fluid-Fluid Coexistence Regions of Charged Membranes.
27288275	6	35	theme	liquid-disordered	1006:1022	arg1	coexistence					1024:1034	liquid-ordered and liquid-disordered coexistence	987:1034	liquid-ordered and liquid-disordered coexistence	987:1034	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	9	36	theme	salt	1324:1327	arg1	effect					1314:1319	a substantial effect	1300:1319	a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains	1300:1535	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	6	37	dep	buffer	857:862	arg1	both					865:868	both	865:868	both	865:868	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	6	37	dep	buffer	857:862	arg1	present					902:908	present	902:908	present	902:908	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	9	38	theme	protein	1430:1436	arg1	adsorption					1438:1447	protein adsorption	1430:1447	protein adsorption onto these membranes	1430:1468	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	4	39	theme	epifluorescence	544:558	arg1	microscopy					560:569	epifluorescence microscopy	544:569	epifluorescence microscopy	544:569	The phase diagram was determined using epifluorescence microscopy for vesicle populations with symmetric and asymmetric solution compositions across the membranes.
27288275	8	40	theme	miscibility	1159:1169	arg1	temperatures					1171:1182	the miscibility temperatures	1155:1182	the miscibility temperatures	1155:1182	Moreover, we found that the miscibility temperatures markedly increased for vesicles in the presence of symmetric and asymmetric salt solutions.
27288275	6	41	theme	phase	812:816	arg1	diagram					818:824	the phase diagram	808:824	the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane)	808:929	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	2	42	theme	lipid	399:403	arg1	dioleoylphosphatidylglycerol					405:432	the negatively charged lipid dioleoylphosphatidylglycerol	376:432	the negatively charged lipid dioleoylphosphatidylglycerol	376:432	To mimic charged biological membranes, we studied phase separation and domain formation in GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol.
27288275	6	43	theme	liquid-ordered	987:1000	arg1	coexistence					1024:1034	liquid-ordered and liquid-disordered coexistence	987:1034	liquid-ordered and liquid-disordered coexistence	987:1034	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	5	44	theme	membrane	727:734	arg1	state					742:746	the membrane phase state	723:746	the membrane phase state	723:746	Trans-membrane solution asymmetry was found to affect the membrane phase state.
27288275	6	45	dep	both	865:868	arg1	symmetrically					870:882	symmetrically	870:882	symmetrically	870:882	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	2	46	theme	biological	223:232	arg1	membranes					234:242	charged biological membranes	215:242	charged biological membranes	215:242	To mimic charged biological membranes, we studied phase separation and domain formation in GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol.
27288275	1	47	theme	giant	123:127	arg1	vesicles					141:148	giant unilamellar vesicles	123:148	giant unilamellar vesicles (GUVs)	123:155	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs) leads to the formation of intramembrane domains.
27288275	1	47	theme	giant	123:127	arg1	GUVs					151:154	GUVs	151:154	GUVs	151:154	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs) leads to the formation of intramembrane domains.
27288275	0	48	theme	Coexistence	47:57	arg1	Regions					59:65	Fluid-Fluid Coexistence Regions	35:65	Fluid-Fluid Coexistence Regions of Charged Membranes	35:86	Solution Asymmetry and Salt Expand Fluid-Fluid Coexistence Regions of Charged Membranes.
27288275	5	49	theme	phase	736:740	arg1	state					742:746	the membrane phase state	723:746	the membrane phase state	723:746	Trans-membrane solution asymmetry was found to affect the membrane phase state.
27288275	9	50	theme	asymmetry	1342:1350	arg1	effect					1314:1319	a substantial effect	1300:1319	a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains	1300:1535	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	2	51	theme	charged	215:221	arg1	membranes					234:242	charged biological membranes	215:242	charged biological membranes	215:242	To mimic charged biological membranes, we studied phase separation and domain formation in GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol.
27288275	7	52	theme	single	1074:1079	arg1	GUVs					1081:1084	single GUVs	1074:1084	single GUVs using microfluidics and confocal microscopy	1074:1128	These observations were confirmed on single GUVs using microfluidics and confocal microscopy.
27288275	1	53	theme	unilamellar	129:139	arg1	vesicles					141:148	giant unilamellar vesicles	123:148	giant unilamellar vesicles (GUVs)	123:155	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs) leads to the formation of intramembrane domains.
27288275	1	53	theme	unilamellar	129:139	arg1	GUVs					151:154	GUVs	151:154	GUVs	151:154	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs) leads to the formation of intramembrane domains.
27288275	1	54	from	separation	109:118	arg1	vesicles					141:148	giant unilamellar vesicles	123:148	giant unilamellar vesicles (GUVs)	123:155	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs) leads to the formation of intramembrane domains.
27288275	1	54	from	separation	109:118	arg1	GUVs					151:154	GUVs	151:154	GUVs	151:154	Liquid-liquid phase separation in giant unilamellar vesicles (GUVs) leads to the formation of intramembrane domains.
27288275	6	55	theme	conditions	796:805	arg1	case					778:781	the case	774:781	the case of salt-free conditions	774:805	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	3	56	theme	high-saline	485:495	arg1	solutions					460:468	solutions	460:468	solutions of sucrose and high-saline buffer	460:502	The GUVs were exposed to solutions of sucrose and high-saline buffer.
27288275	7	57	theme	confocal	1110:1117	arg1	microscopy					1119:1128	confocal microscopy	1110:1128	confocal microscopy	1110:1128	These observations were confirmed on single GUVs using microfluidics and confocal microscopy.
27288275	2	58	theme	phase	256:260	arg1	separation					262:271	phase separation	256:271	phase separation	256:271	To mimic charged biological membranes, we studied phase separation and domain formation in GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol.
27288275	6	59	theme	salt-free	786:794	arg1	conditions					796:805	salt-free conditions	786:805	salt-free conditions	786:805	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	2	60	theme	mixtures	319:326	arg1	GUVs					297:300	GUVs	297:300	GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol	297:432	To mimic charged biological membranes, we studied phase separation and domain formation in GUVs of ternary lipid mixtures composed of egg sphingomyelin, cholesterol, and the negatively charged lipid dioleoylphosphatidylglycerol.
27288275	0	61	theme	Charged	70:76	arg1	Membranes					78:86	Charged Membranes	70:86	Charged Membranes	70:86	Solution Asymmetry and Salt Expand Fluid-Fluid Coexistence Regions of Charged Membranes.
27288275	9	62	theme	phase	1359:1363	arg1	behavior					1365:1372	the phase behavior	1355:1372	the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains	1355:1535	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	3	63	dep	sucrose	473:479	arg1	buffer					497:502	buffer	497:502	buffer	497:502	The GUVs were exposed to solutions of sucrose and high-saline buffer.
27288275	3	64	theme	sucrose	473:479	arg1	solutions					460:468	solutions	460:468	solutions of sucrose and high-saline buffer	460:502	The GUVs were exposed to solutions of sucrose and high-saline buffer.
27288275	6	65	theme	extended	968:975	arg1	region					977:982	a significantly extended region	952:982	a significantly extended region of liquid-ordered and liquid-disordered coexistence	952:1034	Furthermore, compared to the case of salt-free conditions, the phase diagram in the presence of high-saline buffer (both symmetrically or asymmetrically present across the membrane) was found to exhibit a significantly extended region of liquid-ordered and liquid-disordered coexistence.
27288275	9	66	from	effect	1314:1319	arg1	behavior					1365:1372	the phase behavior	1355:1372	the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains	1355:1535	Our results demonstrate a substantial effect of salt and solution asymmetry on the phase behavior of charged membranes, which has direct implications for protein adsorption onto these membranes and for the repartitioning of proteins within the membrane domains.
27288275	8	67	theme	symmetric	1235:1243	arg1	solutions					1265:1273	symmetric and asymmetric salt solutions	1235:1273	symmetric and asymmetric salt solutions	1235:1273	Moreover, we found that the miscibility temperatures markedly increased for vesicles in the presence of symmetric and asymmetric salt solutions.
25982680	2	0	theme	series	495:500	arg1	platforms					587:595	gene-activated scaffold platforms	563:595	gene-activated scaffold platforms with potential	563:610	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	2	0	theme	series	495:500	arg1	potential					480:488	the potential	476:488	the potential of a series of collagen-based scaffolds, developed in our laboratory,	476:558	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	4	1	theme	standard	957:964	arg1	vector					966:971	a non-viral gold standard vector	940:971	a non-viral gold standard vector	940:971	A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects.
25982680	4	1	theme	standard	957:964	arg1	polyethyleneimine					915:931	polyethyleneimine	915:931	polyethyleneimine (PEI)	915:937	A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects.
25982680	5	2	from	MSCs	1153:1156	arg1	expression					1137:1146	sustained transgene expression	1117:1146	sustained transgene expression from MSCs seeded on the scaffolds	1117:1180	When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency.
25982680	2	3	theme	proteins	388:395	arg1	quantities					362:371	supraphysiological quantities	343:371	supraphysiological quantities of recombinant proteins	343:395	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	4	4	theme	gold	952:955	arg1	vector					966:971	a non-viral gold standard vector	940:971	a non-viral gold standard vector	940:971	A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects.
25982680	4	4	theme	gold	952:955	arg1	polyethyleneimine					915:931	polyethyleneimine	915:931	polyethyleneimine (PEI)	915:937	A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects.
25982680	5	5	theme	sustained	1117:1125	arg1	expression					1137:1146	sustained transgene expression	1117:1146	sustained transgene expression from MSCs seeded on the scaffolds	1117:1180	When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency.
25982680	4	6	theme	%	859:859	arg1	efficiency					842:851	A transfection efficiency	827:851	A transfection efficiency of >45%	827:859	A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects.
25982680	2	7	theme	recombinant	376:386	arg1	proteins					388:395	recombinant proteins	376:395	recombinant proteins	376:395	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	6	8	theme	scaffold	1359:1366	arg1	composition					1368:1378	the scaffold composition	1355:1378	the scaffold composition	1355:1378	These results demonstrate that by simply varying the scaffold composition and the gene (or combinations thereof) chosen; the system has potential for a myriad of therapeutic applications.
25982680	5	9	theme	transgene	1127:1135	arg1	expression					1137:1146	sustained transgene expression	1117:1146	sustained transgene expression from MSCs seeded on the scaffolds	1117:1180	When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency.
25982680	2	10	with	platforms	587:595	arg1	potential					602:610	potential	602:610	potential	602:610	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	3	11	theme	crustaceans	738:748	arg1	shells					728:733	the shells	724:733	the shells of crustaceans	724:748	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	3	12	attach	derived	711:717	arg2	material					702:709	a biocompatible material	686:709	a biocompatible material derived from the shells of crustaceans	686:748	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	3	12	attach	derived	711:717	arg2	chitosan					676:683	chitosan	676:683	chitosan	676:683	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	3	12	attach	derived	711:717	arg1	shells					728:733	the shells	724:733	the shells of crustaceans	724:748	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	2	13	theme	applications	645:656	arg1	range					617:621	a range	615:621	a range of tissue engineering applications	615:656	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	1	14	theme	tissue	206:211	arg1	formation					213:221	tissue formation	206:221	tissue formation	206:221	Biomaterial scaffolds that support cell infiltration and tissue formation can also function as platforms for the delivery of therapeutics such as drugs, proteins, and genes.
25982680	0	15	theme	collagen-based	123:136	arg1	scaffolds					138:146	collagen-based scaffolds	123:146	collagen-based scaffolds	123:146	Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds.
25982680	2	16	theme	engineering	633:643	arg1	applications					645:656	tissue engineering applications	626:656	tissue engineering applications	626:656	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	2	17	theme	side	419:422	arg1	effects					424:430	adverse side effects	411:430	adverse side effects	411:430	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	4	18	theme	side	1000:1003	arg1	effects					1005:1011	cytotoxic side effects	990:1011	cytotoxic side effects	990:1011	A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects.
25982680	5	19	theme	collagen-based	1091:1104	arg1	scaffolds					1106:1114	collagen-based scaffolds	1091:1114	collagen-based scaffolds	1091:1114	When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency.
25982680	2	20	theme	tissue	626:631	arg1	applications					645:656	tissue engineering applications	626:656	tissue engineering applications	626:656	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	4	21	theme	non-viral	942:950	arg1	vector					966:971	a non-viral gold standard vector	940:971	a non-viral gold standard vector	940:971	A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects.
25982680	4	21	theme	non-viral	942:950	arg1	polyethyleneimine					915:931	polyethyleneimine	915:931	polyethyleneimine (PEI)	915:937	A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects.
25982680	2	22	theme	adverse	411:417	arg1	effects					424:430	adverse side effects	411:430	adverse side effects	411:430	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	4	23	theme	cytotoxic	990:998	arg1	effects					1005:1011	cytotoxic side effects	990:1011	cytotoxic side effects	990:1011	A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects.
25982680	3	24	theme	gene	756:759	arg1	vector					770:775	a gene delivery vector	754:775	a gene delivery vector	754:775	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	2	25	theme	burst	326:330	arg1	release					332:338	burst release	326:338	burst release of supraphysiological quantities of recombinant proteins	326:395	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	5	26	theme	transfection	1281:1292	arg1	efficiency					1294:1303	transfection efficiency	1281:1303	transfection efficiency	1281:1303	When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency.
25982680	2	27	theme	study	455:459	arg1	objective					437:445	the objective	433:445	the objective of this study	433:459	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	3	28	theme	delivery	761:768	arg1	vector					770:775	a gene delivery vector	754:775	a gene delivery vector	754:775	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	5	29	contain	had	1264:1266	arg1	composition					1236:1246	the composition	1232:1246	the composition of the scaffold	1232:1262	When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency.
25982680	5	29	contain	had	1264:1266	arg2	effect					1271:1276	an effect	1268:1276	an effect	1268:1276	When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency.
25982680	6	30	dep	gene	1388:1391	arg1	combinations					1397:1408	combinations	1397:1408	combinations thereof	1397:1416	These results demonstrate that by simply varying the scaffold composition and the gene (or combinations thereof) chosen; the system has potential for a myriad of therapeutic applications.
25982680	5	31	theme	scaffold	1255:1262	arg1	composition					1236:1246	the composition	1232:1246	the composition of the scaffold	1232:1262	When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency.
25982680	0	32	theme	platform	41:48	arg1	Development					0:10	Development	0:10	Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA	0:104	Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds.
25982680	2	33	theme	quantities	362:371	arg1	release					332:338	burst release	326:338	burst release of supraphysiological quantities of recombinant proteins	326:395	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	6	34	theme	applications	1480:1491	arg1	myriad					1458:1463	a myriad	1456:1463	a myriad of therapeutic applications	1456:1491	These results demonstrate that by simply varying the scaffold composition and the gene (or combinations thereof) chosen; the system has potential for a myriad of therapeutic applications.
25982680	0	35	theme	scaffold	32:39	arg1	platform					41:48	a gene-activated scaffold platform	15:48	a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA	15:104	Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds.
25982680	0	36	theme	gene-activated	17:30	arg1	platform					41:48	a gene-activated scaffold platform	15:48	a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA	15:104	Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds.
25982680	2	37	theme	scaffolds	520:528	arg1	series					495:500	a series	493:500	a series of collagen-based scaffolds, developed in our laboratory,	493:558	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	0	38	theme	tissue	54:59	arg1	applications					73:84	tissue engineering applications	54:84	tissue engineering applications using chitosan-pDNA	54:104	Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds.
25982680	6	39	contain	has	1438:1440	arg1	system					1431:1436	the system	1427:1436	the system	1427:1436	These results demonstrate that by simply varying the scaffold composition and the gene (or combinations thereof) chosen; the system has potential for a myriad of therapeutic applications.
25982680	6	39	contain	has	1438:1440	arg2	potential					1442:1450	potential	1442:1450	potential for a myriad of therapeutic applications	1442:1491	These results demonstrate that by simply varying the scaffold composition and the gene (or combinations thereof) chosen; the system has potential for a myriad of therapeutic applications.
25982680	4	40	theme	transfection	829:840	arg1	efficiency					842:851	A transfection efficiency	827:851	A transfection efficiency of >45%	827:859	A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects.
25982680	1	41	theme	Biomaterial	149:159	arg1	scaffolds					161:169	Biomaterial scaffolds	149:169	Biomaterial scaffolds that support cell infiltration and tissue formation	149:221	Biomaterial scaffolds that support cell infiltration and tissue formation can also function as platforms for the delivery of therapeutics such as drugs, proteins, and genes.
25982680	1	41	theme	Biomaterial	149:159	arg1	platforms					244:252	platforms	244:252	platforms for the delivery of therapeutics such as drugs, proteins, and genes	244:320	Biomaterial scaffolds that support cell infiltration and tissue formation can also function as platforms for the delivery of therapeutics such as drugs, proteins, and genes.
25982680	6	42	theme	therapeutic	1468:1478	arg1	applications					1480:1491	therapeutic applications	1468:1491	therapeutic applications	1468:1491	These results demonstrate that by simply varying the scaffold composition and the gene (or combinations thereof) chosen; the system has potential for a myriad of therapeutic applications.
25982680	2	43	theme	scaffold	578:585	arg1	potential					480:488	the potential	476:488	the potential of a series of collagen-based scaffolds, developed in our laboratory,	476:558	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	2	43	theme	scaffold	578:585	arg1	platforms					587:595	gene-activated scaffold platforms	563:595	gene-activated scaffold platforms with potential	563:610	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	3	44	theme	biocompatible	688:700	arg1	material					702:709	a biocompatible material	686:709	a biocompatible material derived from the shells of crustaceans	686:748	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	3	44	theme	biocompatible	688:700	arg1	chitosan					676:683	chitosan	676:683	chitosan	676:683	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	2	45	theme	supraphysiological	343:360	arg1	quantities					362:371	supraphysiological quantities	343:371	supraphysiological quantities of recombinant proteins	343:395	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	2	46	theme	gene-activated	563:576	arg1	potential					480:488	the potential	476:488	the potential of a series of collagen-based scaffolds, developed in our laboratory,	476:558	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	2	46	theme	gene-activated	563:576	arg1	platforms					587:595	gene-activated scaffold platforms	563:595	gene-activated scaffold platforms with potential	563:610	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	3	47	theme	chitosan	676:683	arg1	potential					663:671	The potential	659:671	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector	659:775	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	5	48	theme	optimised	1023:1031	arg1	nanoparticles					1042:1054	the optimised chitosan nanoparticles	1019:1054	the optimised chitosan nanoparticles	1019:1054	When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency.
25982680	2	49	theme	collagen-based	505:518	arg1	scaffolds					520:528	collagen-based scaffolds	505:528	collagen-based scaffolds	505:528	As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications.
25982680	5	50	theme	chitosan	1033:1040	arg1	nanoparticles					1042:1054	the optimised chitosan nanoparticles	1019:1054	the optimised chitosan nanoparticles	1019:1054	When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency.
25982680	3	51	theme	mesenchymal	796:806	arg1	cells					813:817	mesenchymal stem cells	796:817	mesenchymal stem cells (MSCs)	796:824	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	3	51	theme	mesenchymal	796:806	arg1	MSCs					820:823	MSCs	820:823	MSCs	820:823	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	0	52	theme	engineering	61:71	arg1	applications					73:84	tissue engineering applications	54:84	tissue engineering applications using chitosan-pDNA	54:104	Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds.
25982680	5	53	theme	scaffolds	1106:1114	arg1	series					1081:1086	a series	1079:1086	a series of collagen-based scaffolds	1079:1114	When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency.
25982680	3	54	theme	stem	808:811	arg1	cells					813:817	mesenchymal stem cells	796:817	mesenchymal stem cells (MSCs)	796:824	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	3	54	theme	stem	808:811	arg1	MSCs					820:823	MSCs	820:823	MSCs	820:823	The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs).
25982680	1	55	theme	cell	184:187	arg1	infiltration					189:200	cell infiltration	184:200	cell infiltration	184:200	Biomaterial scaffolds that support cell infiltration and tissue formation can also function as platforms for the delivery of therapeutics such as drugs, proteins, and genes.
25982680	1	56	theme	therapeutics	274:285	arg1	delivery					262:269	the delivery	258:269	the delivery of therapeutics such as drugs, proteins, and genes	258:320	Biomaterial scaffolds that support cell infiltration and tissue formation can also function as platforms for the delivery of therapeutics such as drugs, proteins, and genes.
26794753	4	0	from	Immobilisation	632:645	arg1	microcapsules					725:737	chitosan microcapsules	716:737	chitosan microcapsules	716:737	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	4	0	from	Immobilisation	632:645	arg1	weed					707:710	weed	707:710	weed	707:710	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	4	0	from	Immobilisation	632:645	arg1	maize					697:701	maize	697:701	maize	697:701	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	8	1	theme	Cr	1126:1127	arg1	42.57					1091:1095	42.57	1091:1095	42.57	1091:1095	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	1	2	theme	unexploited	167:177	arg1	biomass					179:185	unexploited biomass	167:185	unexploited biomass for heavy metal removal	167:209	Designing effective chitosan-based biosorbents from unexploited biomass for heavy metal removal has received much attention over the past decade.
26794753	9	3	theme	concentration	1306:1318	arg1	experiments					1244:1254	Sorption experiments	1235:1254	Sorption experiments	1235:1254	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	3	theme	concentration	1306:1318	arg1	function					1276:1283	a function	1274:1283	a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution	1274:1451	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	0	4	theme	chitosan	67:74	arg1	microcapsules					76:88	chitosan microcapsules	67:88	chitosan microcapsules for heavy metal removal	67:112	Microfungal spores (Ustilago maydis and U. digitariae) immobilised chitosan microcapsules for heavy metal removal.
26794753	9	5	theme	solution	1444:1451	arg1	time					1340:1343	contact time	1332:1343	contact time (60-480min)	1332:1355	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	5	theme	solution	1444:1451	arg1	0.05-0.25g					1412:1421	0.05-0.25g	1412:1421	0.05-0.25g	1412:1421	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	5	theme	solution	1444:1451	arg1	concentration					1306:1318	initial metal ion concentration	1288:1318	initial metal ion concentration (2-10mg/L)	1288:1329	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	5	theme	solution	1444:1451	arg1	amount					1389:1394	amount	1389:1394	amount of the sorbent (0.05-0.25g)	1389:1422	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	5	theme	solution	1444:1451	arg1	2-10mg/L					1321:1328	2-10mg/L	1321:1328	2-10mg/L	1321:1328	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	5	theme	solution	1444:1451	arg1	solution					1444:1451	the metal solution	1434:1451	the metal solution	1434:1451	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	5	theme	solution	1444:1451	arg1	60-480min					1346:1354	60-480min	1346:1354	60-480min	1346:1354	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	5	theme	solution	1444:1451	arg1	pH					1428:1429	pH	1428:1429	pH of the metal solution	1428:1451	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	5	theme	solution	1444:1451	arg1	sorbent					1403:1409	the sorbent	1399:1409	the sorbent (0.05-0.25g)	1399:1422	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	5	theme	solution	1444:1451	arg1	temperature					1358:1368	temperature	1358:1368	temperature (25, 35 and 45°C)	1358:1386	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	3	6	from	findings	541:548	arg1	performance					574:584	heavy metal sorption performance	553:584	heavy metal sorption performance of chitosan/Ustilago composite microcapsules	553:629	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	0	7	theme	heavy	94:98	arg1	removal					106:112	heavy metal removal	94:112	heavy metal removal	94:112	Microfungal spores (Ustilago maydis and U. digitariae) immobilised chitosan microcapsules for heavy metal removal.
26794753	9	8	dep	temperature	1358:1368	arg1	45°C					1382:1385	45°C	1382:1385	45°C	1382:1385	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	8	dep	temperature	1358:1368	arg1	35					1375:1376	35	1375:1376	35	1375:1376	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	8	9	theme	Cd	1098:1099	arg1	42.57					1091:1095	42.57	1091:1095	42.57	1091:1095	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	5	10	link	cross-linked	790:801	arg1	microcapsules					803:815	The cross-linked microcapsules	786:815	The cross-linked microcapsules	786:815	The cross-linked microcapsules were characterised using scanning electron microscopy, FT-IR spectroscopy and thermogravimetric analysis.
26794753	3	11	theme	metal	559:563	arg1	performance					574:584	heavy metal sorption performance	553:584	heavy metal sorption performance of chitosan/Ustilago composite microcapsules	553:629	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	4	12	theme	glutaraldehyde	756:769	arg1	cross-linking					771:783	glutaraldehyde cross-linking	756:783	glutaraldehyde cross-linking	756:783	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	3	13	theme	chitosan	509:516	arg1	matrix					518:523	cross-linked chitosan matrix	496:523	cross-linked chitosan matrix	496:523	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	4	14	from	maize	697:701	arg1	Immobilisation					632:645	Immobilisation	632:645	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules	632:737	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	4	14	from	maize	697:701	arg1	maydis					659:664	Ustilago maydis	650:664	Ustilago maydis	650:664	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	4	14	from	maize	697:701	arg1	spores					684:689	U. digitariae spores	670:689	U. digitariae spores (from maize and weed)	670:711	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	4	15	theme	digitariae	673:682	arg1	spores					684:689	U. digitariae spores	670:689	U. digitariae spores (from maize and weed)	670:711	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	9	16	theme	metal	1296:1300	arg1	2-10mg/L					1321:1328	2-10mg/L	1321:1328	2-10mg/L	1321:1328	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	16	theme	metal	1296:1300	arg1	concentration					1306:1318	initial metal ion concentration	1288:1318	initial metal ion concentration (2-10mg/L)	1288:1329	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	5	17	theme	thermogravimetric	895:911	arg1	analysis					913:920	thermogravimetric analysis	895:920	thermogravimetric analysis	895:920	The cross-linked microcapsules were characterised using scanning electron microscopy, FT-IR spectroscopy and thermogravimetric analysis.
26794753	9	18	theme	time	1340:1343	arg1	experiments					1244:1254	Sorption experiments	1235:1254	Sorption experiments	1235:1254	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	18	theme	time	1340:1343	arg1	function					1276:1283	a function	1274:1283	a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution	1274:1451	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	10	19	theme	better	1494:1499	arg1	performance					1501:1511	better performance	1494:1511	better performance	1494:1511	The microcapsules with spores exhibited better performance over the plain chitosan beads, demonstrating their potential use in water treatment.
26794753	0	20	theme	metal	100:104	arg1	removal					106:112	heavy metal removal	94:112	heavy metal removal	94:112	Microfungal spores (Ustilago maydis and U. digitariae) immobilised chitosan microcapsules for heavy metal removal.
26794753	8	21	dep	investigated	1011:1022	arg1	16.43					1178:1182	16.43	1178:1182	16.43	1178:1182	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	8	21	dep	investigated	1011:1022	arg1	15.04mg/g					1210:1218	15.04mg/g	1210:1218	15.04mg/g	1210:1218	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	8	21	dep	investigated	1011:1022	arg1	Cu					1069:1070	Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68	1069:1153	Cu	1069:1070	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	8	21	dep	investigated	1011:1022	arg1	Zn					1188:1189	Zn(II): 30.73, 60.81	1188:1207	Zn	1188:1189	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	9	22	theme	sorbent	1403:1409	arg1	time					1340:1343	contact time	1332:1343	contact time (60-480min)	1332:1355	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	22	theme	sorbent	1403:1409	arg1	0.05-0.25g					1412:1421	0.05-0.25g	1412:1421	0.05-0.25g	1412:1421	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	22	theme	sorbent	1403:1409	arg1	concentration					1306:1318	initial metal ion concentration	1288:1318	initial metal ion concentration (2-10mg/L)	1288:1329	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	22	theme	sorbent	1403:1409	arg1	amount					1389:1394	amount	1389:1394	amount of the sorbent (0.05-0.25g)	1389:1422	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	22	theme	sorbent	1403:1409	arg1	2-10mg/L					1321:1328	2-10mg/L	1321:1328	2-10mg/L	1321:1328	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	22	theme	sorbent	1403:1409	arg1	solution					1444:1451	the metal solution	1434:1451	the metal solution	1434:1451	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	22	theme	sorbent	1403:1409	arg1	60-480min					1346:1354	60-480min	1346:1354	60-480min	1346:1354	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	22	theme	sorbent	1403:1409	arg1	pH					1428:1429	pH	1428:1429	pH of the metal solution	1428:1451	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	22	theme	sorbent	1403:1409	arg1	sorbent					1403:1409	the sorbent	1399:1409	the sorbent (0.05-0.25g)	1399:1422	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	22	theme	sorbent	1403:1409	arg1	temperature					1358:1368	temperature	1358:1368	temperature (25, 35 and 45°C)	1358:1386	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	1	23	theme	heavy	191:195	arg1	removal					203:209	heavy metal removal	191:209	heavy metal removal	191:209	Designing effective chitosan-based biosorbents from unexploited biomass for heavy metal removal has received much attention over the past decade.
26794753	4	24	theme	spores	684:689	arg1	Immobilisation					632:645	Immobilisation	632:645	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules	632:737	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	3	25	theme	sorption	565:572	arg1	performance					574:584	heavy metal sorption performance	553:584	heavy metal sorption performance of chitosan/Ustilago composite microcapsules	553:629	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	1	26	theme	metal	197:201	arg1	removal					203:209	heavy metal removal	191:209	heavy metal removal	191:209	Designing effective chitosan-based biosorbents from unexploited biomass for heavy metal removal has received much attention over the past decade.
26794753	0	27	theme	Microfungal	0:10	arg1	spores					12:17	Microfungal spores	0:17	Microfungal spores (Ustilago maydis and U. digitariae)	0:53	Microfungal spores (Ustilago maydis and U. digitariae) immobilised chitosan microcapsules for heavy metal removal.
26794753	0	27	theme	Microfungal	0:10	arg1	maydis					29:34	Ustilago maydis	20:34	Ustilago maydis	20:34	Microfungal spores (Ustilago maydis and U. digitariae) immobilised chitosan microcapsules for heavy metal removal.
26794753	0	27	theme	Microfungal	0:10	arg1	digitariae					43:52	digitariae	43:52	digitariae	43:52	Microfungal spores (Ustilago maydis and U. digitariae) immobilised chitosan microcapsules for heavy metal removal.
26794753	10	28	theme	chitosan	1528:1535	arg1	beads					1537:1541	the plain chitosan beads	1518:1541	the plain chitosan beads	1518:1541	The microcapsules with spores exhibited better performance over the plain chitosan beads, demonstrating their potential use in water treatment.
26794753	2	29	theme	plant	306:310	arg1	Ustilago					261:268	Ustilago	261:268	Ustilago	261:268	Ustilago, loose smut, is a ubiquitous fungal plant pathogen infecting over 4000 species including maize and weed.
26794753	2	29	theme	plant	306:310	arg1	pathogen					312:319	a ubiquitous fungal plant pathogen	286:319	a ubiquitous fungal plant pathogen infecting over 4000 species including maize and weed	286:372	Ustilago, loose smut, is a ubiquitous fungal plant pathogen infecting over 4000 species including maize and weed.
26794753	8	30	theme	digitariae	981:990	arg1	microcapsules					992:1004	digitariae microcapsules	981:1004	digitariae microcapsules	981:1004	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	0	31	theme	Ustilago	20:27	arg1	spores					12:17	Microfungal spores	0:17	Microfungal spores (Ustilago maydis and U. digitariae)	0:53	Microfungal spores (Ustilago maydis and U. digitariae) immobilised chitosan microcapsules for heavy metal removal.
26794753	0	31	theme	Ustilago	20:27	arg1	maydis					29:34	Ustilago maydis	20:34	Ustilago maydis	20:34	Microfungal spores (Ustilago maydis and U. digitariae) immobilised chitosan microcapsules for heavy metal removal.
26794753	9	32	theme	Sorption	1235:1242	arg1	experiments					1244:1254	Sorption experiments	1235:1254	Sorption experiments	1235:1254	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	32	theme	Sorption	1235:1242	arg1	function					1276:1283	a function	1274:1283	a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution	1274:1451	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	33	theme	metal	1438:1442	arg1	solution					1444:1451	the metal solution	1434:1451	the metal solution	1434:1451	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	2	34	theme	fungal	299:304	arg1	Ustilago					261:268	Ustilago	261:268	Ustilago	261:268	Ustilago, loose smut, is a ubiquitous fungal plant pathogen infecting over 4000 species including maize and weed.
26794753	2	34	theme	fungal	299:304	arg1	pathogen					312:319	a ubiquitous fungal plant pathogen	286:319	a ubiquitous fungal plant pathogen infecting over 4000 species including maize and weed	286:372	Ustilago, loose smut, is a ubiquitous fungal plant pathogen infecting over 4000 species including maize and weed.
26794753	9	35	theme	pH	1428:1429	arg1	experiments					1244:1254	Sorption experiments	1235:1254	Sorption experiments	1235:1254	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	35	theme	pH	1428:1429	arg1	function					1276:1283	a function	1274:1283	a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution	1274:1451	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	3	36	theme	spores	467:472	arg1	spores					417:422	the spores	413:422	the spores of the phytopathogenic microfungi Ustilago spores	413:472	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	8	37	theme	cross-linked	1040:1051	arg1	beads					1062:1066	cross-linked chitosan beads	1040:1066	cross-linked chitosan beads	1040:1066	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	10	38	theme	water	1581:1585	arg1	treatment					1587:1595	water treatment	1581:1595	water treatment	1581:1595	The microcapsules with spores exhibited better performance over the plain chitosan beads, demonstrating their potential use in water treatment.
26794753	6	39	theme	chitosan-U	946:955	arg1	capacities					932:941	Sorption capacities	923:941	Sorption capacities of chitosan-U.	923:956	Sorption capacities of chitosan-U.
26794753	2	40	theme	ubiquitous	288:297	arg1	Ustilago					261:268	Ustilago	261:268	Ustilago	261:268	Ustilago, loose smut, is a ubiquitous fungal plant pathogen infecting over 4000 species including maize and weed.
26794753	2	40	theme	ubiquitous	288:297	arg1	pathogen					312:319	a ubiquitous fungal plant pathogen	286:319	a ubiquitous fungal plant pathogen infecting over 4000 species including maize and weed	286:372	Ustilago, loose smut, is a ubiquitous fungal plant pathogen infecting over 4000 species including maize and weed.
26794753	4	41	theme	maydis	659:664	arg1	Immobilisation					632:645	Immobilisation	632:645	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules	632:737	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	4	42	theme	chitosan	716:723	arg1	microcapsules					725:737	chitosan microcapsules	716:737	chitosan microcapsules	716:737	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	10	43	theme	plain	1522:1526	arg1	beads					1537:1541	the plain chitosan beads	1518:1541	the plain chitosan beads	1518:1541	The microcapsules with spores exhibited better performance over the plain chitosan beads, demonstrating their potential use in water treatment.
26794753	1	44	theme	much	224:227	arg1	attention					229:237	much attention	224:237	much attention	224:237	Designing effective chitosan-based biosorbents from unexploited biomass for heavy metal removal has received much attention over the past decade.
26794753	3	45	theme	composite	607:615	arg1	microcapsules					617:629	chitosan/Ustilago composite microcapsules	589:629	chitosan/Ustilago composite microcapsules	589:629	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	8	46	dep	Cu	1069:1070	arg1	42.57					1091:1095	42.57	1091:1095	42.57	1091:1095	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	8	46	dep	Cu	1069:1070	arg1	Ni					1156:1157	Ni	1156:1157	Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni	1069:1157	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	3	47	theme	microfungi	447:456	arg1	spores					467:472	the phytopathogenic microfungi Ustilago spores	427:472	the phytopathogenic microfungi Ustilago spores	427:472	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	4	48	from	weed	707:710	arg1	Immobilisation					632:645	Immobilisation	632:645	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules	632:737	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	4	48	from	weed	707:710	arg1	maydis					659:664	Ustilago maydis	650:664	Ustilago maydis	650:664	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	4	48	from	weed	707:710	arg1	spores					684:689	U. digitariae spores	670:689	U. digitariae spores (from maize and weed)	670:711	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	3	49	theme	microcapsules	617:629	arg1	performance					574:584	heavy metal sorption performance	553:584	heavy metal sorption performance of chitosan/Ustilago composite microcapsules	553:629	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	3	50	theme	Ustilago	458:465	arg1	spores					467:472	the phytopathogenic microfungi Ustilago spores	427:472	the phytopathogenic microfungi Ustilago spores	427:472	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	5	51	theme	scanning	842:849	arg1	microscopy					860:869	scanning electron microscopy	842:869	scanning electron microscopy	842:869	The cross-linked microcapsules were characterised using scanning electron microscopy, FT-IR spectroscopy and thermogravimetric analysis.
26794753	9	52	theme	contact	1332:1338	arg1	time					1340:1343	contact time	1332:1343	contact time (60-480min)	1332:1355	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	52	theme	contact	1332:1338	arg1	60-480min					1346:1354	60-480min	1346:1354	60-480min	1346:1354	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	6	53	theme	Sorption	923:930	arg1	capacities					932:941	Sorption capacities	923:941	Sorption capacities of chitosan-U.	923:956	Sorption capacities of chitosan-U.
26794753	5	54	theme	FT-IR	872:876	arg1	spectroscopy					878:889	FT-IR spectroscopy	872:889	FT-IR spectroscopy	872:889	The cross-linked microcapsules were characterised using scanning electron microscopy, FT-IR spectroscopy and thermogravimetric analysis.
26794753	5	55	theme	electron	851:858	arg1	microscopy					860:869	scanning electron microscopy	842:869	scanning electron microscopy	842:869	The cross-linked microcapsules were characterised using scanning electron microscopy, FT-IR spectroscopy and thermogravimetric analysis.
26794753	8	56	theme	7.87	1120:1123	arg1	Cd					1098:1099	Cd(II): 49.46, 53.96	1098:1117	Cd	1098:1099	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	3	57	theme	chitosan/Ustilago	589:605	arg1	microcapsules					617:629	chitosan/Ustilago composite microcapsules	589:629	chitosan/Ustilago composite microcapsules	589:629	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	3	58	theme	cross-linked	496:507	arg1	matrix					518:523	cross-linked chitosan matrix	496:523	cross-linked chitosan matrix	496:523	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	10	59	from	use	1574:1576	arg1	treatment					1587:1595	water treatment	1581:1595	water treatment	1581:1595	The microcapsules with spores exhibited better performance over the plain chitosan beads, demonstrating their potential use in water treatment.
26794753	8	60	link	cross-linked	1040:1051	arg1	beads					1062:1066	cross-linked chitosan beads	1040:1066	cross-linked chitosan beads	1040:1066	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	2	61	theme	loose	271:275	arg1	Ustilago					261:268	Ustilago	261:268	Ustilago	261:268	Ustilago, loose smut, is a ubiquitous fungal plant pathogen infecting over 4000 species including maize and weed.
26794753	2	61	theme	loose	271:275	arg1	smut					277:280	loose smut	271:280	loose smut	271:280	Ustilago, loose smut, is a ubiquitous fungal plant pathogen infecting over 4000 species including maize and weed.
26794753	1	62	theme	effective	125:133	arg1	biosorbents					150:160	effective chitosan-based biosorbents	125:160	effective chitosan-based biosorbents	125:160	Designing effective chitosan-based biosorbents from unexploited biomass for heavy metal removal has received much attention over the past decade.
26794753	3	63	theme	heavy	553:557	arg1	performance					574:584	heavy metal sorption performance	553:584	heavy metal sorption performance of chitosan/Ustilago composite microcapsules	553:629	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	3	64	link	cross-linked	496:507	arg1	matrix					518:523	cross-linked chitosan matrix	496:523	cross-linked chitosan matrix	496:523	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	9	65	theme	amount	1389:1394	arg1	experiments					1244:1254	Sorption experiments	1235:1254	Sorption experiments	1235:1254	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	65	theme	amount	1389:1394	arg1	function					1276:1283	a function	1274:1283	a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution	1274:1451	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	8	66	theme	chitosan	1053:1060	arg1	beads					1062:1066	cross-linked chitosan beads	1040:1066	cross-linked chitosan beads	1040:1066	digitariae microcapsules were investigated and compared to cross-linked chitosan beads: Cu(II): 66.72, 69.26, 42.57; Cd(II): 49.46, 53.96, 7.87; Cr(III): 35.88, 49.40, 43.68; Ni(II): 41.67, 33.46, 16.43 and Zn(II): 30.73, 60.81, 15.04mg/g, respectively.
26794753	1	67	theme	chitosan-based	135:148	arg1	biosorbents					150:160	effective chitosan-based biosorbents	125:160	effective chitosan-based biosorbents	125:160	Designing effective chitosan-based biosorbents from unexploited biomass for heavy metal removal has received much attention over the past decade.
26794753	9	68	theme	initial	1288:1294	arg1	2-10mg/L					1321:1328	2-10mg/L	1321:1328	2-10mg/L	1321:1328	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	68	theme	initial	1288:1294	arg1	concentration					1306:1318	initial metal ion concentration	1288:1318	initial metal ion concentration (2-10mg/L)	1288:1329	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	69	theme	temperature	1358:1368	arg1	experiments					1244:1254	Sorption experiments	1235:1254	Sorption experiments	1235:1254	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	69	theme	temperature	1358:1368	arg1	function					1276:1283	a function	1274:1283	a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution	1274:1451	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	4	70	theme	U.	670:671	arg1	spores					684:689	U. digitariae spores	670:689	U. digitariae spores (from maize and weed)	670:711	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	0	71	dep	spores	12:17	arg1	spores					12:17	Microfungal spores	0:17	Microfungal spores (Ustilago maydis and U. digitariae)	0:53	Microfungal spores (Ustilago maydis and U. digitariae) immobilised chitosan microcapsules for heavy metal removal.
26794753	0	71	dep	spores	12:17	arg1	maydis					29:34	Ustilago maydis	20:34	Ustilago maydis	20:34	Microfungal spores (Ustilago maydis and U. digitariae) immobilised chitosan microcapsules for heavy metal removal.
26794753	0	71	dep	spores	12:17	arg1	digitariae					43:52	digitariae	43:52	digitariae	43:52	Microfungal spores (Ustilago maydis and U. digitariae) immobilised chitosan microcapsules for heavy metal removal.
26794753	4	72	theme	Ustilago	650:657	arg1	maydis					659:664	Ustilago maydis	650:664	Ustilago maydis	650:664	Immobilisation of Ustilago maydis and U. digitariae spores (from maize and weed) in chitosan microcapsules was achieved via glutaraldehyde cross-linking.
26794753	10	73	theme	potential	1564:1572	arg1	use					1574:1576	their potential use	1558:1576	their potential use in water treatment	1558:1595	The microcapsules with spores exhibited better performance over the plain chitosan beads, demonstrating their potential use in water treatment.
26794753	3	74	theme	phytopathogenic	431:445	arg1	spores					467:472	the phytopathogenic microfungi Ustilago spores	427:472	the phytopathogenic microfungi Ustilago spores	427:472	This study aimed to establish whether the spores of the phytopathogenic microfungi Ustilago spores can be immobilised in cross-linked chitosan matrix, and it reports findings on heavy metal sorption performance of chitosan/Ustilago composite microcapsules.
26794753	1	75	theme	past	248:251	arg1	decade					253:258	the past decade	244:258	the past decade	244:258	Designing effective chitosan-based biosorbents from unexploited biomass for heavy metal removal has received much attention over the past decade.
26794753	5	76	theme	cross-linked	790:801	arg1	microcapsules					803:815	The cross-linked microcapsules	786:815	The cross-linked microcapsules	786:815	The cross-linked microcapsules were characterised using scanning electron microscopy, FT-IR spectroscopy and thermogravimetric analysis.
26794753	10	77	with	microcapsules	1458:1470	arg1	spores					1477:1482	spores	1477:1482	spores	1477:1482	The microcapsules with spores exhibited better performance over the plain chitosan beads, demonstrating their potential use in water treatment.
26794753	9	78	theme	ion	1302:1304	arg1	2-10mg/L					1321:1328	2-10mg/L	1321:1328	2-10mg/L	1321:1328	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
26794753	9	78	theme	ion	1302:1304	arg1	concentration					1306:1318	initial metal ion concentration	1288:1318	initial metal ion concentration (2-10mg/L)	1288:1329	Sorption experiments were conducted as a function of initial metal ion concentration (2-10mg/L), contact time (60-480min), temperature (25, 35 and 45°C), amount of the sorbent (0.05-0.25g) and pH of the metal solution.
24689355	0	0	theme	cane	97:100	arg1	bagasse					102:108	sugar cane bagasse	91:108	sugar cane bagasse	91:108	Topochemistry of environmentally friendly pretreatments to enhance enzymatic hydrolysis of sugar cane bagasse to fermentable sugar.
24689355	4	1	theme	surface	754:760	arg1	coverage					762:769	surface coverage	754:769	surface coverage by lignin and extractives	754:795	Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment.
24689355	1	2	theme	alkaline	153:160	arg1	pretreatments					184:196	dilute alkaline and alkaline peroxide pretreatments	146:196	dilute alkaline and alkaline peroxide pretreatments	146:196	In this work, dilute alkaline and alkaline peroxide pretreatments were conducted in comparison with hydrotropic pretreatment to improve the delignification of bagasse prior to enzymatic hydrolysis.
24689355	6	3	theme	enzymatic	1063:1071	arg1	hydrolysis					1073:1082	the enzymatic hydrolysis	1059:1082	the enzymatic hydrolysis	1059:1082	However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
24689355	0	4	theme	sugar	91:95	arg1	bagasse					102:108	sugar cane bagasse	91:108	sugar cane bagasse	91:108	Topochemistry of environmentally friendly pretreatments to enhance enzymatic hydrolysis of sugar cane bagasse to fermentable sugar.
24689355	6	5	theme	14.3	1147:1150	arg1	%					1151:1151	83.9 and 14.3%	1138:1151	%	1151:1151	However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
24689355	6	6	contain	had	1030:1032	arg1	treatment					1020:1028	the hydrotropic treatment	1004:1028	the hydrotropic treatment	1004:1028	However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
24689355	6	6	contain	had	1030:1032	arg2	advantages					1040:1049	clear advantages	1034:1049	clear advantages	1034:1049	However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
24689355	4	7	theme	xylan	818:822	arg1	removal					807:813	dramatic removal	798:813	dramatic removal of xylan	798:822	Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment.
24689355	4	7	theme	xylan	818:822	arg1	decrease					742:749	the decrease	738:749	the decrease of surface coverage by lignin and extractives	738:795	Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment.
24689355	0	8	theme	bagasse	102:108	arg1	hydrolysis					77:86	enzymatic hydrolysis	67:86	enzymatic hydrolysis of sugar cane bagasse to fermentable sugar	67:129	Topochemistry of environmentally friendly pretreatments to enhance enzymatic hydrolysis of sugar cane bagasse to fermentable sugar.
24689355	3	9	theme	lignin	544:549	arg1	distribution					528:539	The surface distribution	516:539	The surface distribution of lignin and extractives on the bagasse fiber	516:586	The surface distribution of lignin and extractives on the bagasse fiber was significantly changed by dilute alkaline, alkaline peroxide, and hydrotropic pretreatments.
24689355	1	10	theme	bagasse	291:297	arg1	delignification					272:286	the delignification	268:286	the delignification of bagasse prior to enzymatic hydrolysis	268:327	In this work, dilute alkaline and alkaline peroxide pretreatments were conducted in comparison with hydrotropic pretreatment to improve the delignification of bagasse prior to enzymatic hydrolysis.
24689355	1	11	theme	alkaline	166:173	arg1	peroxide					175:182	alkaline peroxide	166:182	alkaline peroxide	166:182	In this work, dilute alkaline and alkaline peroxide pretreatments were conducted in comparison with hydrotropic pretreatment to improve the delignification of bagasse prior to enzymatic hydrolysis.
24689355	0	12	theme	fermentable	113:123	arg1	sugar					125:129	fermentable sugar	113:129	fermentable sugar	113:129	Topochemistry of environmentally friendly pretreatments to enhance enzymatic hydrolysis of sugar cane bagasse to fermentable sugar.
24689355	3	13	theme	bagasse	574:580	arg1	fiber					582:586	the bagasse fiber	570:586	the bagasse fiber	570:586	The surface distribution of lignin and extractives on the bagasse fiber was significantly changed by dilute alkaline, alkaline peroxide, and hydrotropic pretreatments.
24689355	1	14	theme	peroxide	175:182	arg1	pretreatments					184:196	dilute alkaline and alkaline peroxide pretreatments	146:196	dilute alkaline and alkaline peroxide pretreatments	146:196	In this work, dilute alkaline and alkaline peroxide pretreatments were conducted in comparison with hydrotropic pretreatment to improve the delignification of bagasse prior to enzymatic hydrolysis.
24689355	6	15	theme	hydrotropic	1008:1018	arg1	treatment					1020:1028	the hydrotropic treatment	1004:1028	the hydrotropic treatment	1004:1028	However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
24689355	6	16	theme	clear	1034:1038	arg1	advantages					1040:1049	clear advantages	1034:1049	clear advantages	1034:1049	However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
24689355	2	17	theme	chemical	342:349	arg1	composition					351:361	The surface chemical composition	330:361	The surface chemical composition of bagasse after pretreatments	330:392	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	2	18	theme	photoelectron	420:432	arg1	spectroscopy					434:445	X-ray photoelectron spectroscopy	414:445	X-ray photoelectron spectroscopy (XPS)	414:451	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	2	18	theme	photoelectron	420:432	arg1	XPS					448:450	XPS	448:450	XPS	448:450	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	2	19	theme	mass	486:489	arg1	ToF-SIMS					505:512	ToF-SIMS	505:512	ToF-SIMS	505:512	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	2	19	theme	mass	486:489	arg1	spectrometry					491:502	time-of-flight secondary ion mass spectrometry	457:502	time-of-flight secondary ion mass spectrometry (ToF-SIMS)	457:513	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	1	20	theme	prior	299:303	arg1	bagasse					291:297	bagasse	291:297	bagasse prior to enzymatic hydrolysis	291:327	In this work, dilute alkaline and alkaline peroxide pretreatments were conducted in comparison with hydrotropic pretreatment to improve the delignification of bagasse prior to enzymatic hydrolysis.
24689355	3	21	from	distribution	528:539	arg1	fiber					582:586	the bagasse fiber	570:586	the bagasse fiber	570:586	The surface distribution of lignin and extractives on the bagasse fiber was significantly changed by dilute alkaline, alkaline peroxide, and hydrotropic pretreatments.
24689355	2	22	theme	surface	334:340	arg1	composition					351:361	The surface chemical composition	330:361	The surface chemical composition of bagasse after pretreatments	330:392	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	2	23	theme	X-ray	414:418	arg1	spectroscopy					434:445	X-ray photoelectron spectroscopy	414:445	X-ray photoelectron spectroscopy (XPS)	414:451	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	2	23	theme	X-ray	414:418	arg1	XPS					448:450	XPS	448:450	XPS	448:450	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	2	24	theme	ion	482:484	arg1	ToF-SIMS					505:512	ToF-SIMS	505:512	ToF-SIMS	505:512	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	2	24	theme	ion	482:484	arg1	spectrometry					491:502	time-of-flight secondary ion mass spectrometry	457:502	time-of-flight secondary ion mass spectrometry (ToF-SIMS)	457:513	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	1	25	with	comparison	216:225	arg1	pretreatment					244:255	hydrotropic pretreatment	232:255	hydrotropic pretreatment	232:255	In this work, dilute alkaline and alkaline peroxide pretreatments were conducted in comparison with hydrotropic pretreatment to improve the delignification of bagasse prior to enzymatic hydrolysis.
24689355	2	26	theme	time-of-flight	457:470	arg1	ToF-SIMS					505:512	ToF-SIMS	505:512	ToF-SIMS	505:512	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	2	26	theme	time-of-flight	457:470	arg1	spectrometry					491:502	time-of-flight secondary ion mass spectrometry	457:502	time-of-flight secondary ion mass spectrometry (ToF-SIMS)	457:513	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	0	27	theme	environmentally	17:31	arg1	friendly					33:40	environmentally friendly	17:40	environmentally friendly	17:40	Topochemistry of environmentally friendly pretreatments to enhance enzymatic hydrolysis of sugar cane bagasse to fermentable sugar.
24689355	1	28	theme	enzymatic	308:316	arg1	hydrolysis					318:327	enzymatic hydrolysis	308:327	enzymatic hydrolysis	308:327	In this work, dilute alkaline and alkaline peroxide pretreatments were conducted in comparison with hydrotropic pretreatment to improve the delignification of bagasse prior to enzymatic hydrolysis.
24689355	4	29	theme	dramatic	798:805	arg1	removal					807:813	dramatic removal	798:813	dramatic removal of xylan	798:822	Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment.
24689355	4	29	theme	dramatic	798:805	arg1	decrease					742:749	the decrease	738:749	the decrease of surface coverage by lignin and extractives	738:795	Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment.
24689355	4	30	theme	coverage	762:769	arg1	removal					807:813	dramatic removal	798:813	dramatic removal of xylan	798:822	Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment.
24689355	4	30	theme	coverage	762:769	arg1	decrease					742:749	the decrease	738:749	the decrease of surface coverage by lignin and extractives	738:795	Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment.
24689355	3	31	theme	alkaline	634:641	arg1	peroxide					643:650	alkaline peroxide	634:650	alkaline peroxide	634:650	The surface distribution of lignin and extractives on the bagasse fiber was significantly changed by dilute alkaline, alkaline peroxide, and hydrotropic pretreatments.
24689355	6	32	theme	bagasse	1125:1131	arg1	xylan					1105:1109	xylan	1105:1109	xylan	1105:1109	However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
24689355	6	32	theme	bagasse	1125:1131	arg1	glucan					1094:1099	glucan	1094:1099	glucan	1094:1099	However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
24689355	3	33	theme	dilute	617:622	arg1	alkaline					624:631	dilute alkaline	617:631	dilute alkaline	617:631	The surface distribution of lignin and extractives on the bagasse fiber was significantly changed by dilute alkaline, alkaline peroxide, and hydrotropic pretreatments.
24689355	5	34	theme	Fiber	908:912	arg1	morphology					914:923	Fiber morphology	908:923	Fiber morphology after pretreatments	908:943	Fiber morphology after pretreatments was more favorable for enzyme hydrolysis as well.
24689355	0	35	theme	friendly	33:40	arg1	Topochemistry					0:12	Topochemistry	0:12	Topochemistry of environmentally friendly	0:40	Topochemistry of environmentally friendly pretreatments to enhance enzymatic hydrolysis of sugar cane bagasse to fermentable sugar.
24689355	0	36	theme	enzymatic	67:75	arg1	hydrolysis					77:86	enzymatic hydrolysis	67:86	enzymatic hydrolysis of sugar cane bagasse to fermentable sugar	67:129	Topochemistry of environmentally friendly pretreatments to enhance enzymatic hydrolysis of sugar cane bagasse to fermentable sugar.
24689355	4	37	theme	fiber	874:878	arg1	surface					880:886	the fiber surface	870:886	the fiber surface	870:886	Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment.
24689355	5	38	theme	enzyme	968:973	arg1	hydrolysis					975:984	enzyme hydrolysis	968:984	enzyme hydrolysis	968:984	Fiber morphology after pretreatments was more favorable for enzyme hydrolysis as well.
24689355	2	39	theme	bagasse	366:372	arg1	composition					351:361	The surface chemical composition	330:361	The surface chemical composition of bagasse after pretreatments	330:392	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	4	40	theme	more	844:847	arg1	cellulose					849:857	more cellulose	844:857	more cellulose exposed on the fiber surface after pretreatment	844:905	Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment.
24689355	3	41	theme	hydrotropic	657:667	arg1	pretreatments					669:681	hydrotropic pretreatments	657:681	hydrotropic pretreatments	657:681	The surface distribution of lignin and extractives on the bagasse fiber was significantly changed by dilute alkaline, alkaline peroxide, and hydrotropic pretreatments.
24689355	1	42	theme	hydrotropic	232:242	arg1	pretreatment					244:255	hydrotropic pretreatment	232:255	hydrotropic pretreatment	232:255	In this work, dilute alkaline and alkaline peroxide pretreatments were conducted in comparison with hydrotropic pretreatment to improve the delignification of bagasse prior to enzymatic hydrolysis.
24689355	6	43	theme	pretreated	1114:1123	arg1	bagasse					1125:1131	pretreated bagasse	1114:1131	pretreated bagasse	1114:1131	However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
24689355	3	44	theme	surface	520:526	arg1	distribution					528:539	The surface distribution	516:539	The surface distribution of lignin and extractives on the bagasse fiber	516:586	The surface distribution of lignin and extractives on the bagasse fiber was significantly changed by dilute alkaline, alkaline peroxide, and hydrotropic pretreatments.
24689355	4	45	theme	Hydrotropic	684:694	arg1	pretreatment					696:707	Hydrotropic pretreatment	684:707	Hydrotropic pretreatment	684:707	Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment.
24689355	3	46	theme	extractives	555:565	arg1	distribution					528:539	The surface distribution	516:539	The surface distribution of lignin and extractives on the bagasse fiber	516:586	The surface distribution of lignin and extractives on the bagasse fiber was significantly changed by dilute alkaline, alkaline peroxide, and hydrotropic pretreatments.
24689355	6	47	theme	83.9	1138:1141	arg1	%					1151:1151	83.9 and 14.3%	1138:1151	%	1151:1151	However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
24689355	2	48	theme	secondary	472:480	arg1	ToF-SIMS					505:512	ToF-SIMS	505:512	ToF-SIMS	505:512	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	2	48	theme	secondary	472:480	arg1	spectrometry					491:502	time-of-flight secondary ion mass spectrometry	457:502	time-of-flight secondary ion mass spectrometry (ToF-SIMS)	457:513	The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS).
24689355	1	49	theme	dilute	146:151	arg1	alkaline					153:160	dilute alkaline	146:160	dilute alkaline	146:160	In this work, dilute alkaline and alkaline peroxide pretreatments were conducted in comparison with hydrotropic pretreatment to improve the delignification of bagasse prior to enzymatic hydrolysis.
27693418	8	0	theme	cell	1105:1108	arg1	stimulation					1110:1120	cell stimulation	1105:1120	cell stimulation with TNF-α	1105:1131	In addition, we confirmed that cell stimulation with TNF-α was accompanied by the secretion of an active form of Sulf-1.
27693418	11	1	theme	HS	1604:1605	arg1	properties					1618:1627	HS biological properties	1604:1627	HS biological properties	1604:1627	Silencing the expression of Sulf-1 by RNA interference restored the responses induced by FGF-1, which indicated that TNF-α-mediated induction of the sulfatase indeed resulted in alterations of HS biological properties.
27693418	7	2	theme	Sulf-1	964:969	arg1	expression					971:980	Sulf-1 expression	964:980	Sulf-1 expression	964:980	Real-time PCR analysis revealed that TNF-α increased Sulf-1 expression in a dose- and time-dependent manner, via a mechanism involving NF-ĸB, ERK1/2 and p38 MAPK.
27693418	2	3	theme	factors	353:359	arg1	variety					335:341	a variety	333:341	a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1	333:418	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	2	3	theme	factors	353:359	arg1	factors					353:359	growth factors	346:359	growth factors	346:359	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	2	3	theme	factors	353:359	arg1	FGF					413:415	fibroblast growth factor (FGF)-1	387:418	fibroblast growth factor (FGF)-1	387:418	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	2	3	theme	factors	353:359	arg1	morphogens					365:374	morphogens	365:374	morphogens	365:374	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	5	4	from	expression	666:675	arg1	fibroblasts					701:711	human MRC-5 fibroblasts	689:711	human MRC-5 fibroblasts	689:711	In this study, we examined the effect of this inflammatory cytokine on the expression of Sulfs in human MRC-5 fibroblasts.
27693418	4	5	theme	Tumour-necrosis	492:506	arg1	TNF					516:518	TNF	516:518	TNF	516:518	Tumour-necrosis factor (TNF)-α plays an important role in regulating the behaviour of fibroblasts.
27693418	4	5	theme	Tumour-necrosis	492:506	arg1	factor					508:513	Tumour-necrosis factor	492:513	Tumour-necrosis factor (TNF)	492:519	Tumour-necrosis factor (TNF)-α plays an important role in regulating the behaviour of fibroblasts.
27693418	6	6	theme	strong	777:782	arg1	reduction					784:792	a strong reduction	775:792	a strong reduction	775:792	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	2	7	theme	growth	346:351	arg1	factors					353:359	growth factors	346:359	growth factors	346:359	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	2	7	theme	growth	346:351	arg1	FGF					413:415	fibroblast growth factor (FGF)-1	387:418	fibroblast growth factor (FGF)-1	387:418	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	10	8	theme	TNF-α-treated	1354:1366	arg1	cells					1374:1378	TNF-α-treated MRC-5 cells	1354:1378	TNF-α-treated MRC-5 cells	1354:1378	We showed that ERK1/2 activation and cell proliferation were markedly reduced in TNF-α-treated MRC-5 cells compared with untreated cells.
27693418	5	9	theme	Sulfs	680:684	arg1	expression					666:675	the expression	662:675	the expression of Sulfs in human MRC-5 fibroblasts	662:711	In this study, we examined the effect of this inflammatory cytokine on the expression of Sulfs in human MRC-5 fibroblasts.
27693418	7	10	theme	PCR	921:923	arg1	analysis					925:932	Real-time PCR analysis	911:932	Real-time PCR analysis	911:932	Real-time PCR analysis revealed that TNF-α increased Sulf-1 expression in a dose- and time-dependent manner, via a mechanism involving NF-ĸB, ERK1/2 and p38 MAPK.
27693418	12	11	theme	TNF-α	1696:1700	arg1	stimulation					1702:1712	TNF-α stimulation	1696:1712	TNF-α stimulation	1696:1712	Taken together, our results indicate that Sulf-1 is responsive to TNF-α stimulation and may function as an autocrine regulator of fibroblast expansion in the course of an inflammatory response.
27693418	11	12	theme	RNA	1449:1451	arg1	interference					1453:1464	RNA interference	1449:1464	RNA interference	1449:1464	Silencing the expression of Sulf-1 by RNA interference restored the responses induced by FGF-1, which indicated that TNF-α-mediated induction of the sulfatase indeed resulted in alterations of HS biological properties.
27693418	7	13	theme	time-dependent	997:1010	arg1	manner					1012:1017	time-dependent manner	997:1017	time-dependent manner	997:1017	Real-time PCR analysis revealed that TNF-α increased Sulf-1 expression in a dose- and time-dependent manner, via a mechanism involving NF-ĸB, ERK1/2 and p38 MAPK.
27693418	10	14	theme	cell	1310:1313	arg1	proliferation					1315:1327	cell proliferation	1310:1327	cell proliferation	1310:1327	We showed that ERK1/2 activation and cell proliferation were markedly reduced in TNF-α-treated MRC-5 cells compared with untreated cells.
27693418	5	15	theme	human	689:693	arg1	fibroblasts					701:711	human MRC-5 fibroblasts	689:711	human MRC-5 fibroblasts	689:711	In this study, we examined the effect of this inflammatory cytokine on the expression of Sulfs in human MRC-5 fibroblasts.
27693418	7	16	theme	p38	1064:1066	arg1	MAPK					1068:1071	p38 MAPK	1064:1071	p38 MAPK	1064:1071	Real-time PCR analysis revealed that TNF-α increased Sulf-1 expression in a dose- and time-dependent manner, via a mechanism involving NF-ĸB, ERK1/2 and p38 MAPK.
27693418	2	17	theme	HS	287:288	arg1	sequences					290:298	specific HS sequences	278:298	specific HS sequences	278:298	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	2	18	theme	growth	398:403	arg1	FGF					413:415	fibroblast growth factor (FGF)-1	387:418	fibroblast growth factor (FGF)-1	387:418	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	5	19	theme	MRC-5	695:699	arg1	fibroblasts					701:711	human MRC-5 fibroblasts	689:711	human MRC-5 fibroblasts	689:711	In this study, we examined the effect of this inflammatory cytokine on the expression of Sulfs in human MRC-5 fibroblasts.
27693418	11	20	theme	biological	1607:1616	arg1	properties					1618:1627	HS biological properties	1604:1627	HS biological properties	1604:1627	Silencing the expression of Sulf-1 by RNA interference restored the responses induced by FGF-1, which indicated that TNF-α-mediated induction of the sulfatase indeed resulted in alterations of HS biological properties.
27693418	6	21	theme	TNF-α-treated	748:760	arg1	cells					762:766	TNF-α-treated cells	748:766	TNF-α-treated cells	748:766	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	2	22	theme	specific	278:285	arg1	sequences					290:298	specific HS sequences	278:298	specific HS sequences	278:298	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	2	23	theme	fibroblast	387:396	arg1	FGF					413:415	fibroblast growth factor (FGF)-1	387:418	fibroblast growth factor (FGF)-1	387:418	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	6	24	theme	disaccharide	840:851	arg1	disaccharide					840:851	the trisulfated UA2S-GlcNS6S disaccharide	811:851	the trisulfated UA2S-GlcNS6S disaccharide	811:851	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	6	24	theme	disaccharide	840:851	arg1	amount					801:806	the amount	797:806	the amount of the trisulfated UA2S-GlcNS6S disaccharide	797:851	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	12	25	theme	fibroblast	1760:1769	arg1	expansion					1771:1779	fibroblast expansion	1760:1779	fibroblast expansion	1760:1779	Taken together, our results indicate that Sulf-1 is responsive to TNF-α stimulation and may function as an autocrine regulator of fibroblast expansion in the course of an inflammatory response.
27693418	3	26	from	functions	456:464	arg1	inflammation					478:489	inflammation	478:489	inflammation	478:489	However, little is known about the functions of Sulfs in inflammation.
27693418	0	27	theme	Tumour-necrosis	0:14	arg1	factor-α					16:23	Tumour-necrosis factor-α	0:23	Tumour-necrosis factor-α	0:23	Tumour-necrosis factor-α induces heparan sulfate 6-O-endosulfatase 1 (Sulf-1) expression in fibroblasts.
27693418	10	28	theme	MRC-5	1368:1372	arg1	cells					1374:1378	TNF-α-treated MRC-5 cells	1354:1378	TNF-α-treated MRC-5 cells	1354:1378	We showed that ERK1/2 activation and cell proliferation were markedly reduced in TNF-α-treated MRC-5 cells compared with untreated cells.
27693418	8	29	theme	Sulf-1	1187:1192	arg1	form					1179:1182	an active form	1169:1182	an active form of Sulf-1	1169:1192	In addition, we confirmed that cell stimulation with TNF-α was accompanied by the secretion of an active form of Sulf-1.
27693418	6	30	theme	HS	740:741	arg1	analysis					728:735	Compositional analysis	714:735	Compositional analysis of HS from TNF-α-treated cells	714:766	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	1	31	theme	critical	178:185	arg1	regulators					187:196	critical regulators	178:196	critical regulators of many physiological and pathological processes	178:245	Heparan sulfate (HS) 6-O-endosulfatases (Sulfs) have emerged recently as critical regulators of many physiological and pathological processes.
27693418	0	32	theme	heparan	33:39	arg1	Sulf-1					70:75	Sulf-1	70:75	Sulf-1	70:75	Tumour-necrosis factor-α induces heparan sulfate 6-O-endosulfatase 1 (Sulf-1) expression in fibroblasts.
27693418	0	32	theme	heparan	33:39	arg1	sulfate					41:47	heparan sulfate 6-O-endosulfatase 1	33:67	heparan sulfate 6-O-endosulfatase 1 (Sulf-1) expression in fibroblasts	33:102	Tumour-necrosis factor-α induces heparan sulfate 6-O-endosulfatase 1 (Sulf-1) expression in fibroblasts.
27693418	11	33	theme	properties	1618:1627	arg1	alterations					1589:1599	alterations	1589:1599	alterations of HS biological properties	1589:1627	Silencing the expression of Sulf-1 by RNA interference restored the responses induced by FGF-1, which indicated that TNF-α-mediated induction of the sulfatase indeed resulted in alterations of HS biological properties.
27693418	8	34	with	stimulation	1110:1120	arg1	TNF-α					1127:1131	TNF-α	1127:1131	TNF-α	1127:1131	In addition, we confirmed that cell stimulation with TNF-α was accompanied by the secretion of an active form of Sulf-1.
27693418	1	35	theme	Heparan	105:111	arg1	HS					122:123	HS	122:123	HS	122:123	Heparan sulfate (HS) 6-O-endosulfatases (Sulfs) have emerged recently as critical regulators of many physiological and pathological processes.
27693418	1	35	theme	Heparan	105:111	arg1	sulfate					113:119	Heparan sulfate	105:119	Heparan sulfate (HS) 6-O-endosulfatases (Sulfs)	105:151	Heparan sulfate (HS) 6-O-endosulfatases (Sulfs) have emerged recently as critical regulators of many physiological and pathological processes.
27693418	10	36	theme	untreated	1394:1402	arg1	cells					1404:1408	untreated cells	1394:1408	untreated cells	1394:1408	We showed that ERK1/2 activation and cell proliferation were markedly reduced in TNF-α-treated MRC-5 cells compared with untreated cells.
27693418	11	37	theme	sulfatase	1560:1568	arg1	induction					1543:1551	TNF-α-mediated induction	1528:1551	TNF-α-mediated induction of the sulfatase	1528:1568	Silencing the expression of Sulf-1 by RNA interference restored the responses induced by FGF-1, which indicated that TNF-α-mediated induction of the sulfatase indeed resulted in alterations of HS biological properties.
27693418	4	38	theme	important	532:540	arg1	role					542:545	an important role	529:545	an important role	529:545	Tumour-necrosis factor (TNF)-α plays an important role in regulating the behaviour of fibroblasts.
27693418	12	39	theme	response	1814:1821	arg1	course					1788:1793	the course	1784:1793	the course of an inflammatory response	1784:1821	Taken together, our results indicate that Sulf-1 is responsive to TNF-α stimulation and may function as an autocrine regulator of fibroblast expansion in the course of an inflammatory response.
27693418	6	40	theme	UA2S-GlcNS6S	827:838	arg1	disaccharide					840:851	the trisulfated UA2S-GlcNS6S disaccharide	811:851	the trisulfated UA2S-GlcNS6S disaccharide	811:851	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	1	41	theme	sulfate	113:119	arg1	Sulfs					146:150	Sulfs	146:150	Sulfs	146:150	Heparan sulfate (HS) 6-O-endosulfatases (Sulfs) have emerged recently as critical regulators of many physiological and pathological processes.
27693418	1	41	theme	sulfate	113:119	arg1	6-O-endosulfatases					126:143	Heparan sulfate (HS) 6-O-endosulfatases	105:143	Heparan sulfate (HS) 6-O-endosulfatases (Sulfs)	105:151	Heparan sulfate (HS) 6-O-endosulfatases (Sulfs) have emerged recently as critical regulators of many physiological and pathological processes.
27693418	0	42	theme	6-O-endosulfatase	49:65	arg1	Sulf-1					70:75	Sulf-1	70:75	Sulf-1	70:75	Tumour-necrosis factor-α induces heparan sulfate 6-O-endosulfatase 1 (Sulf-1) expression in fibroblasts.
27693418	0	42	theme	6-O-endosulfatase	49:65	arg1	sulfate					41:47	heparan sulfate 6-O-endosulfatase 1	33:67	heparan sulfate 6-O-endosulfatase 1 (Sulf-1) expression in fibroblasts	33:102	Tumour-necrosis factor-α induces heparan sulfate 6-O-endosulfatase 1 (Sulf-1) expression in fibroblasts.
27693418	3	43	theme	Sulfs	469:473	arg1	functions					456:464	the functions	452:464	the functions of Sulfs in inflammation	452:489	However, little is known about the functions of Sulfs in inflammation.
27693418	0	44	from	expression	78:87	arg1	fibroblasts					92:102	fibroblasts	92:102	fibroblasts	92:102	Tumour-necrosis factor-α induces heparan sulfate 6-O-endosulfatase 1 (Sulf-1) expression in fibroblasts.
27693418	1	45	theme	many	201:204	arg1	processes					237:245	many physiological and pathological processes	201:245	many physiological and pathological processes	201:245	Heparan sulfate (HS) 6-O-endosulfatases (Sulfs) have emerged recently as critical regulators of many physiological and pathological processes.
27693418	0	46	theme	sulfate	41:47	arg1	expression					78:87	heparan sulfate 6-O-endosulfatase 1 (Sulf-1) expression	33:87	heparan sulfate 6-O-endosulfatase 1 (Sulf-1) expression in fibroblasts	33:102	Tumour-necrosis factor-α induces heparan sulfate 6-O-endosulfatase 1 (Sulf-1) expression in fibroblasts.
27693418	6	47	from	reduction	784:792	arg1	disaccharide					840:851	the trisulfated UA2S-GlcNS6S disaccharide	811:851	the trisulfated UA2S-GlcNS6S disaccharide	811:851	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	6	47	from	reduction	784:792	arg1	amount					801:806	the amount	797:806	the amount of the trisulfated UA2S-GlcNS6S disaccharide	797:851	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	12	48	theme	inflammatory	1801:1812	arg1	response					1814:1821	an inflammatory response	1798:1821	an inflammatory response	1798:1821	Taken together, our results indicate that Sulf-1 is responsive to TNF-α stimulation and may function as an autocrine regulator of fibroblast expansion in the course of an inflammatory response.
27693418	12	49	theme	expansion	1771:1779	arg1	regulator					1747:1755	an autocrine regulator	1734:1755	an autocrine regulator of fibroblast expansion	1734:1779	Taken together, our results indicate that Sulf-1 is responsive to TNF-α stimulation and may function as an autocrine regulator of fibroblast expansion in the course of an inflammatory response.
27693418	12	49	theme	expansion	1771:1779	arg1	Sulf-1					1672:1677	Sulf-1	1672:1677	Sulf-1	1672:1677	Taken together, our results indicate that Sulf-1 is responsive to TNF-α stimulation and may function as an autocrine regulator of fibroblast expansion in the course of an inflammatory response.
27693418	1	50	theme	physiological	206:218	arg1	processes					237:245	many physiological and pathological processes	201:245	many physiological and pathological processes	201:245	Heparan sulfate (HS) 6-O-endosulfatases (Sulfs) have emerged recently as critical regulators of many physiological and pathological processes.
27693418	8	51	theme	form	1179:1182	arg1	secretion					1156:1164	the secretion	1152:1164	the secretion of an active form of Sulf-1	1152:1192	In addition, we confirmed that cell stimulation with TNF-α was accompanied by the secretion of an active form of Sulf-1.
27693418	5	52	from	effect	622:627	arg1	expression					666:675	the expression	662:675	the expression of Sulfs in human MRC-5 fibroblasts	662:711	In this study, we examined the effect of this inflammatory cytokine on the expression of Sulfs in human MRC-5 fibroblasts.
27693418	6	53	theme	Compositional	714:726	arg1	analysis					728:735	Compositional analysis	714:735	Compositional analysis of HS from TNF-α-treated cells	714:766	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	8	54	theme	active	1172:1177	arg1	form					1179:1182	an active form	1169:1182	an active form of Sulf-1	1169:1192	In addition, we confirmed that cell stimulation with TNF-α was accompanied by the secretion of an active form of Sulf-1.
27693418	6	55	theme	selective	872:880	arg1	reaction					882:889	a selective reaction	870:889	a selective reaction of 6-O-desulfation	870:908	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	2	56	theme	variety	335:341	arg1	activities					319:328	the activities	315:328	the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1	315:418	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	6	57	theme	6-O-desulfation	894:908	arg1	reaction					882:889	a selective reaction	870:889	a selective reaction of 6-O-desulfation	870:908	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	5	58	theme	inflammatory	637:648	arg1	cytokine					650:657	this inflammatory cytokine	632:657	this inflammatory cytokine	632:657	In this study, we examined the effect of this inflammatory cytokine on the expression of Sulfs in human MRC-5 fibroblasts.
27693418	7	59	theme	Real-time	911:919	arg1	analysis					925:932	Real-time PCR analysis	911:932	Real-time PCR analysis	911:932	Real-time PCR analysis revealed that TNF-α increased Sulf-1 expression in a dose- and time-dependent manner, via a mechanism involving NF-ĸB, ERK1/2 and p38 MAPK.
27693418	11	60	theme	TNF-α-mediated	1528:1541	arg1	induction					1543:1551	TNF-α-mediated induction	1528:1551	TNF-α-mediated induction of the sulfatase	1528:1568	Silencing the expression of Sulf-1 by RNA interference restored the responses induced by FGF-1, which indicated that TNF-α-mediated induction of the sulfatase indeed resulted in alterations of HS biological properties.
27693418	6	61	from	analysis	728:735	arg1	cells					762:766	TNF-α-treated cells	748:766	TNF-α-treated cells	748:766	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	11	62	theme	Sulf-1	1439:1444	arg1	expression					1425:1434	the expression	1421:1434	the expression of Sulf-1 by RNA interference	1421:1464	Silencing the expression of Sulf-1 by RNA interference restored the responses induced by FGF-1, which indicated that TNF-α-mediated induction of the sulfatase indeed resulted in alterations of HS biological properties.
27693418	5	63	theme	cytokine	650:657	arg1	effect					622:627	the effect	618:627	the effect of this inflammatory cytokine on the expression of Sulfs in human MRC-5 fibroblasts	618:711	In this study, we examined the effect of this inflammatory cytokine on the expression of Sulfs in human MRC-5 fibroblasts.
27693418	12	64	theme	autocrine	1737:1745	arg1	regulator					1747:1755	an autocrine regulator	1734:1755	an autocrine regulator of fibroblast expansion	1734:1779	Taken together, our results indicate that Sulf-1 is responsive to TNF-α stimulation and may function as an autocrine regulator of fibroblast expansion in the course of an inflammatory response.
27693418	12	64	theme	autocrine	1737:1745	arg1	Sulf-1					1672:1677	Sulf-1	1672:1677	Sulf-1	1672:1677	Taken together, our results indicate that Sulf-1 is responsive to TNF-α stimulation and may function as an autocrine regulator of fibroblast expansion in the course of an inflammatory response.
27693418	1	65	theme	pathological	224:235	arg1	processes					237:245	many physiological and pathological processes	201:245	many physiological and pathological processes	201:245	Heparan sulfate (HS) 6-O-endosulfatases (Sulfs) have emerged recently as critical regulators of many physiological and pathological processes.
27693418	6	66	theme	trisulfated	815:825	arg1	disaccharide					840:851	the trisulfated UA2S-GlcNS6S disaccharide	811:851	the trisulfated UA2S-GlcNS6S disaccharide	811:851	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	2	67	theme	factor	405:410	arg1	FGF					413:415	fibroblast growth factor (FGF)-1	387:418	fibroblast growth factor (FGF)-1	387:418	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	10	68	theme	ERK1/2	1288:1293	arg1	activation					1295:1304	ERK1/2 activation	1288:1304	ERK1/2 activation	1288:1304	We showed that ERK1/2 activation and cell proliferation were markedly reduced in TNF-α-treated MRC-5 cells compared with untreated cells.
27693418	2	69	theme	morphogens	365:374	arg1	variety					335:341	a variety	333:341	a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1	333:418	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	2	69	theme	morphogens	365:374	arg1	factors					353:359	growth factors	346:359	growth factors	346:359	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	2	69	theme	morphogens	365:374	arg1	FGF					413:415	fibroblast growth factor (FGF)-1	387:418	fibroblast growth factor (FGF)-1	387:418	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	2	69	theme	morphogens	365:374	arg1	morphogens					365:374	morphogens	365:374	morphogens	365:374	By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1.
27693418	6	70	from	cells	762:766	arg1	HS					740:741	HS	740:741	HS from TNF-α-treated cells	740:766	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	6	70	from	cells	762:766	arg1	analysis					728:735	Compositional analysis	714:735	Compositional analysis of HS from TNF-α-treated cells	714:766	Compositional analysis of HS from TNF-α-treated cells showed a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, which suggested a selective reaction of 6-O-desulfation.
27693418	1	71	theme	processes	237:245	arg1	regulators					187:196	critical regulators	178:196	critical regulators of many physiological and pathological processes	178:245	Heparan sulfate (HS) 6-O-endosulfatases (Sulfs) have emerged recently as critical regulators of many physiological and pathological processes.
27693418	9	72	theme	Sulf-	1220:1224	arg1	function					1208:1215	the function	1204:1215	the function of Sulf- 1	1204:1226	To study the function of Sulf- 1, we examined the responses induced by FGF-1.
27693418	4	73	theme	fibroblasts	578:588	arg1	behaviour					565:573	the behaviour	561:573	the behaviour of fibroblasts	561:588	Tumour-necrosis factor (TNF)-α plays an important role in regulating the behaviour of fibroblasts.
26695273	2	0	theme	acid	481:484	arg1	amount					437:442	amount	437:442	amount of chitosan	437:454	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	0	theme	acid	481:484	arg1	concentration					457:469	concentration	457:469	concentration of acetic acid	457:484	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	0	theme	acid	481:484	arg1	chitosan					447:454	chitosan	447:454	chitosan	447:454	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	0	theme	acid	481:484	arg1	time					499:502	reaction time	490:502	reaction time	490:502	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	0	theme	acid	481:484	arg1	acid					481:484	acetic acid	474:484	acetic acid	474:484	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	4	1	theme	absorption	679:688	arg1	bands					690:694	a distinctive absorption bands	665:694	a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond	665:757	All the separated C-dots peaks display a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond.
26695273	1	2	theme	acetic	232:237	arg1	acid					239:242	glacial acetic acid	224:242	glacial acetic acid	224:242	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	0	3	theme	samples	75:81	arg1	study					39:43	mass spectrometric study	20:43	mass spectrometric study of as-synthesized carbon dots samples	20:81	UHPLC combined with mass spectrometric study of as-synthesized carbon dots samples.
26695273	10	4	theme	storage	1681:1687	arg1	fields					1614:1619	the fields	1610:1619	the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device	1610:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	5	5	dep	composition	800:810	arg1	the					796:798	the	796:798	the	796:798	The obtained chromatograms indicate the composition and complexity of an as-synthesized C-dots sample which is commonly neglected.
26695273	10	6	theme	device	1710:1715	arg1	fields					1614:1619	the fields	1610:1619	the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device	1610:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	4	7	theme	distinctive	667:677	arg1	bands					690:694	a distinctive absorption bands	665:694	a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond	665:757	All the separated C-dots peaks display a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond.
26695273	8	8	theme	PL	1192:1193	arg1	spectra					1195:1201	The PL spectra	1188:1201	The PL spectra of the fractions	1188:1218	The PL spectra of the fractions display emission peaks at 427-446 nm upon excitation at 340 nm.
26695273	7	9	theme	time-of-flight	1139:1152	arg1	spectrometry					1159:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	1095:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1095:1185	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	7	9	theme	time-of-flight	1139:1152	arg1	MS					1183:1184	MALDI-TOF MS	1173:1184	MALDI-TOF MS	1173:1184	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	1	10	dep	synthesize	113:122	arg1	C-dots					137:142	C-dots	137:142	C-dots	137:142	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	8	11	from	nm	1254:1255	arg1	peaks					1237:1241	emission peaks	1228:1241	emission peaks at 427-446 nm	1228:1255	The PL spectra of the fractions display emission peaks at 427-446 nm upon excitation at 340 nm.
26695273	7	12	theme	mass	1154:1157	arg1	spectrometry					1159:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	1095:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1095:1185	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	7	12	theme	mass	1154:1157	arg1	MS					1183:1184	MALDI-TOF MS	1173:1184	MALDI-TOF MS	1173:1184	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	10	13	theme	catalysis	1649:1657	arg1	fields					1614:1619	the fields	1610:1619	the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device	1610:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	4	14	theme	*	734:734	arg1	transition					736:745	the n→π* transition	727:745	the n→π* transition of C=O bond	727:757	All the separated C-dots peaks display a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond.
26695273	7	15	theme	separated	993:1001	arg1	fractions					1010:1018	The separated C-dots fractions	989:1018	The separated C-dots fractions	989:1018	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	2	16	theme	liquid	313:318	arg1	UHPLC					336:340	UHPLC	336:340	UHPLC	336:340	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	16	theme	liquid	313:318	arg1	chromatography					320:333	Ultra-high performance liquid chromatography	290:333	Ultra-high performance liquid chromatography (UHPLC)	290:341	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	10	17	theme	bioimaging	1637:1646	arg1	fields					1614:1619	the fields	1610:1619	the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device	1610:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	9	18	theme	MALDI-TOF	1329:1337	arg1	MS					1339:1340	MALDI-TOF MS	1329:1340	MALDI-TOF MS	1329:1340	The C-dots fractions are fully anatomized by MALDI-TOF MS, displaying their fragmentation mass ion features and indicating the surface functionalities of C-dots.
26695273	7	19	theme	laser	1111:1115	arg1	spectrometry					1159:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	1095:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1095:1185	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	7	19	theme	laser	1111:1115	arg1	MS					1183:1184	MALDI-TOF MS	1173:1184	MALDI-TOF MS	1173:1184	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	2	20	theme	various	398:404	arg1	conditions					416:425	various synthetic conditions	398:425	various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time	398:502	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	20	theme	various	398:404	arg1	amount					437:442	amount	437:442	amount of chitosan	437:454	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	20	theme	various	398:404	arg1	acid					481:484	acetic acid	474:484	acetic acid	474:484	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	20	theme	various	398:404	arg1	chitosan					447:454	chitosan	447:454	chitosan	447:454	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	20	theme	various	398:404	arg1	concentration					457:469	concentration	457:469	concentration of acetic acid	457:484	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	20	theme	various	398:404	arg1	time					499:502	reaction time	490:502	reaction time	490:502	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	1	21	dep	approach	101:108	arg1	synthesize					113:122	synthesize	113:122	to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent	110:268	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	10	22	theme	energy	1674:1679	arg1	storage					1681:1687	energy storage	1674:1687	energy storage	1674:1687	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	4	23	theme	separated	634:642	arg1	peaks					651:655	All the separated C-dots peaks	626:655	All the separated C-dots peaks	626:655	All the separated C-dots peaks display a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond.
26695273	5	24	theme	obtained	764:771	arg1	chromatograms					773:785	The obtained chromatograms	760:785	The obtained chromatograms	760:785	The obtained chromatograms indicate the composition and complexity of an as-synthesized C-dots sample which is commonly neglected.
26695273	7	25	theme	desorption/ionization	1117:1137	arg1	spectrometry					1159:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	1095:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1095:1185	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	7	25	theme	desorption/ionization	1117:1137	arg1	MS					1183:1184	MALDI-TOF MS	1173:1184	MALDI-TOF MS	1173:1184	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	3	26	theme	C-dots	525:530	arg1	samples					532:538	All the as-prepared C-dots samples	505:538	All the as-prepared C-dots samples	505:538	All the as-prepared C-dots samples are complex mixtures of C-dots species which can be separated by UHPLC within 16 min.
26695273	3	26	theme	C-dots	525:530	arg1	mixtures					552:559	complex mixtures	544:559	complex mixtures of C-dots species which can be separated by UHPLC within 16 min	544:623	All the as-prepared C-dots samples are complex mixtures of C-dots species which can be separated by UHPLC within 16 min.
26695273	1	27	theme	microwave-assisted	148:165	arg1	pyrolysis					167:175	microwave-assisted pyrolysis	148:175	microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid	148:242	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	8	28	theme	fractions	1210:1218	arg1	spectra					1195:1201	The PL spectra	1188:1201	The PL spectra of the fractions	1188:1218	The PL spectra of the fractions display emission peaks at 427-446 nm upon excitation at 340 nm.
26695273	2	29	theme	acetic	474:479	arg1	acid					481:484	acetic acid	474:484	acetic acid	474:484	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	0	30	theme	dots	70:73	arg1	samples					75:81	as-synthesized carbon dots samples	48:81	as-synthesized carbon dots samples	48:81	UHPLC combined with mass spectrometric study of as-synthesized carbon dots samples.
26695273	2	31	theme	performance	301:311	arg1	UHPLC					336:340	UHPLC	336:340	UHPLC	336:340	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	31	theme	performance	301:311	arg1	chromatography					320:333	Ultra-high performance liquid chromatography	290:333	Ultra-high performance liquid chromatography (UHPLC)	290:341	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	10	32	theme	C-dots	1554:1559	arg1	product					1561:1567	individual C-dots product	1543:1567	individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device	1543:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	5	33	theme	sample	855:860	arg1	complexity					816:825	complexity	816:825	complexity	816:825	The obtained chromatograms indicate the composition and complexity of an as-synthesized C-dots sample which is commonly neglected.
26695273	5	33	theme	sample	855:860	arg1	composition					800:810	composition	800:810	composition	800:810	The obtained chromatograms indicate the composition and complexity of an as-synthesized C-dots sample which is commonly neglected.
26695273	1	34	theme	condensation	251:262	arg1	agent					264:268	the condensation agent	247:268	the condensation agent	247:268	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	1	34	theme	condensation	251:262	arg1	dots					131:134	carbon dots	124:134	carbon dots	124:134	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	7	35	theme	matrix-assisted	1095:1109	arg1	spectrometry					1159:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	1095:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1095:1185	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	7	35	theme	matrix-assisted	1095:1109	arg1	MS					1183:1184	MALDI-TOF MS	1173:1184	MALDI-TOF MS	1173:1184	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	5	36	theme	as-synthesized	833:846	arg1	sample					855:860	an as-synthesized C-dots sample	830:860	an as-synthesized C-dots sample which is commonly neglected	830:888	The obtained chromatograms indicate the composition and complexity of an as-synthesized C-dots sample which is commonly neglected.
26695273	1	37	theme	fast	86:89	arg1	approach					101:108	A fast and green approach	84:108	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent	84:268	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	4	38	theme	C-dots	644:649	arg1	peaks					651:655	All the separated C-dots peaks	626:655	All the separated C-dots peaks	626:655	All the separated C-dots peaks display a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond.
26695273	8	39	theme	emission	1228:1235	arg1	peaks					1237:1241	emission peaks	1228:1241	emission peaks at 427-446 nm	1228:1255	The PL spectra of the fractions display emission peaks at 427-446 nm upon excitation at 340 nm.
26695273	10	40	contain	have	1579:1582	arg2	applications					1594:1605	potential applications	1584:1605	potential applications	1584:1605	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	10	40	contain	have	1579:1582	arg1	product					1561:1567	individual C-dots product	1543:1567	individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device	1543:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	3	41	theme	complex	544:550	arg1	samples					532:538	All the as-prepared C-dots samples	505:538	All the as-prepared C-dots samples	505:538	All the as-prepared C-dots samples are complex mixtures of C-dots species which can be separated by UHPLC within 16 min.
26695273	3	41	theme	complex	544:550	arg1	mixtures					552:559	complex mixtures	544:559	complex mixtures of C-dots species which can be separated by UHPLC within 16 min	544:623	All the as-prepared C-dots samples are complex mixtures of C-dots species which can be separated by UHPLC within 16 min.
26695273	10	42	theme	UHPLC	1484:1488	arg1	virtues					1473:1479	the virtues	1469:1479	the virtues of UHPLC	1469:1488	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	1	43	theme	precursor	180:188	arg1	chitosan					190:197	precursor chitosan	180:197	precursor chitosan	180:197	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	2	44	theme	Ultra-high	290:299	arg1	UHPLC					336:340	UHPLC	336:340	UHPLC	336:340	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	44	theme	Ultra-high	290:299	arg1	chromatography					320:333	Ultra-high performance liquid chromatography	290:333	Ultra-high performance liquid chromatography (UHPLC)	290:341	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	9	45	theme	fragmentation	1360:1372	arg1	features					1383:1390	their fragmentation mass ion features	1354:1390	their fragmentation mass ion features	1354:1390	The C-dots fractions are fully anatomized by MALDI-TOF MS, displaying their fragmentation mass ion features and indicating the surface functionalities of C-dots.
26695273	3	46	theme	as-prepared	513:523	arg1	samples					532:538	All the as-prepared C-dots samples	505:538	All the as-prepared C-dots samples	505:538	All the as-prepared C-dots samples are complex mixtures of C-dots species which can be separated by UHPLC within 16 min.
26695273	3	46	theme	as-prepared	513:523	arg1	mixtures					552:559	complex mixtures	544:559	complex mixtures of C-dots species which can be separated by UHPLC within 16 min	544:623	All the as-prepared C-dots samples are complex mixtures of C-dots species which can be separated by UHPLC within 16 min.
26695273	1	47	theme	green	95:99	arg1	approach					101:108	A fast and green approach	84:108	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent	84:268	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	1	48	theme	chitosan	190:197	arg1	pyrolysis					167:175	microwave-assisted pyrolysis	148:175	microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid	148:242	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	10	49	theme	chemosensing	1660:1671	arg1	fields					1614:1619	the fields	1610:1619	the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device	1610:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	9	50	theme	mass	1374:1377	arg1	features					1383:1390	their fragmentation mass ion features	1354:1390	their fragmentation mass ion features	1354:1390	The C-dots fractions are fully anatomized by MALDI-TOF MS, displaying their fragmentation mass ion features and indicating the surface functionalities of C-dots.
26695273	10	51	theme	optoelectronics	1694:1708	arg1	device					1710:1715	optoelectronics device	1694:1715	optoelectronics device	1694:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	7	52	theme	MALDI-TOF	1173:1181	arg1	spectrometry					1159:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	1095:1170	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)	1095:1185	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	7	52	theme	MALDI-TOF	1173:1181	arg1	MS					1183:1184	MALDI-TOF MS	1173:1184	MALDI-TOF MS	1173:1184	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	0	53	theme	spectrometric	25:37	arg1	study					39:43	mass spectrometric study	20:43	mass spectrometric study of as-synthesized carbon dots samples	20:81	UHPLC combined with mass spectrometric study of as-synthesized carbon dots samples.
26695273	5	54	theme	C-dots	848:853	arg1	sample					855:860	an as-synthesized C-dots sample	830:860	an as-synthesized C-dots sample which is commonly neglected	830:888	The obtained chromatograms indicate the composition and complexity of an as-synthesized C-dots sample which is commonly neglected.
26695273	10	55	theme	bioanalysis	1624:1634	arg1	fields					1614:1619	the fields	1610:1619	the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device	1610:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	9	56	theme	C-dots	1438:1443	arg1	functionalities					1419:1433	the surface functionalities	1407:1433	the surface functionalities of C-dots	1407:1443	The C-dots fractions are fully anatomized by MALDI-TOF MS, displaying their fragmentation mass ion features and indicating the surface functionalities of C-dots.
26695273	10	57	theme	potential	1584:1592	arg1	applications					1594:1605	potential applications	1584:1605	potential applications	1584:1605	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	0	58	theme	as-synthesized	48:61	arg1	samples					75:81	as-synthesized carbon dots samples	48:81	as-synthesized carbon dots samples	48:81	UHPLC combined with mass spectrometric study of as-synthesized carbon dots samples.
26695273	9	59	theme	ion	1379:1381	arg1	features					1383:1390	their fragmentation mass ion features	1354:1390	their fragmentation mass ion features	1354:1390	The C-dots fractions are fully anatomized by MALDI-TOF MS, displaying their fragmentation mass ion features and indicating the surface functionalities of C-dots.
26695273	10	60	theme	product	1561:1567	arg1	characteristics					1524:1538	the unique characteristics	1513:1538	the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device	1513:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	3	61	theme	species	571:577	arg1	samples					532:538	All the as-prepared C-dots samples	505:538	All the as-prepared C-dots samples	505:538	All the as-prepared C-dots samples are complex mixtures of C-dots species which can be separated by UHPLC within 16 min.
26695273	3	61	theme	species	571:577	arg1	mixtures					552:559	complex mixtures	544:559	complex mixtures of C-dots species which can be separated by UHPLC within 16 min	544:623	All the as-prepared C-dots samples are complex mixtures of C-dots species which can be separated by UHPLC within 16 min.
26695273	2	62	theme	chitosan	447:454	arg1	amount					437:442	amount	437:442	amount of chitosan	437:454	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	62	theme	chitosan	447:454	arg1	concentration					457:469	concentration	457:469	concentration of acetic acid	457:484	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	62	theme	chitosan	447:454	arg1	chitosan					447:454	chitosan	447:454	chitosan	447:454	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	62	theme	chitosan	447:454	arg1	time					499:502	reaction time	490:502	reaction time	490:502	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	62	theme	chitosan	447:454	arg1	acid					481:484	acetic acid	474:484	acetic acid	474:484	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	7	63	theme	photoluminescence	1055:1071	arg1	spectroscopy					1078:1089	photoluminescence (PL) spectroscopy	1055:1089	photoluminescence (PL) spectroscopy	1055:1089	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	7	64	theme	C-dots	1003:1008	arg1	fractions					1010:1018	The separated C-dots fractions	989:1018	The separated C-dots fractions	989:1018	The separated C-dots fractions are collected and characterized by photoluminescence (PL) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
26695273	4	65	from	nm	707:708	arg1	bands					690:694	a distinctive absorption bands	665:694	a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond	665:757	All the separated C-dots peaks display a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond.
26695273	6	66	theme	liquid	921:926	arg1	chromatography					928:941	high-performance liquid chromatography	904:941	high-performance liquid chromatography	904:941	In addition, high-performance liquid chromatography is employed to fractionate the C-dots sample.
26695273	10	67	theme	individual	1543:1552	arg1	product					1561:1567	individual C-dots product	1543:1567	individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device	1543:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	4	68	theme	n→π	731:733	arg1	transition					736:745	the n→π* transition	727:745	the n→π* transition of C=O bond	727:757	All the separated C-dots peaks display a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond.
26695273	1	69	theme	carbon	206:211	arg1	source					213:218	the carbon source	202:218	the carbon source	202:218	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	4	70	theme	C=O	750:752	arg1	bond					754:757	C=O bond	750:757	C=O bond	750:757	All the separated C-dots peaks display a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond.
26695273	4	71	theme	bond	754:757	arg1	transition					736:745	the n→π* transition	727:745	the n→π* transition of C=O bond	727:757	All the separated C-dots peaks display a distinctive absorption bands at 261-271 nm corresponding to the n→π* transition of C=O bond.
26695273	1	72	theme	carbon	124:129	arg1	agent					264:268	the condensation agent	247:268	the condensation agent	247:268	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	1	72	theme	carbon	124:129	arg1	dots					131:134	carbon dots	124:134	carbon dots	124:134	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
26695273	9	73	theme	surface	1411:1417	arg1	functionalities					1419:1433	the surface functionalities	1407:1433	the surface functionalities of C-dots	1407:1443	The C-dots fractions are fully anatomized by MALDI-TOF MS, displaying their fragmentation mass ion features and indicating the surface functionalities of C-dots.
26695273	2	74	theme	reaction	490:497	arg1	time					499:502	reaction time	490:502	reaction time	490:502	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	9	75	theme	C-dots	1288:1293	arg1	fractions					1295:1303	The C-dots fractions	1284:1303	The C-dots fractions	1284:1303	The C-dots fractions are fully anatomized by MALDI-TOF MS, displaying their fragmentation mass ion features and indicating the surface functionalities of C-dots.
26695273	2	76	theme	synthetic	406:414	arg1	conditions					416:425	various synthetic conditions	398:425	various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time	398:502	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	76	theme	synthetic	406:414	arg1	amount					437:442	amount	437:442	amount of chitosan	437:454	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	76	theme	synthetic	406:414	arg1	acid					481:484	acetic acid	474:484	acetic acid	474:484	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	76	theme	synthetic	406:414	arg1	chitosan					447:454	chitosan	447:454	chitosan	447:454	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	76	theme	synthetic	406:414	arg1	concentration					457:469	concentration	457:469	concentration of acetic acid	457:484	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	76	theme	synthetic	406:414	arg1	time					499:502	reaction time	490:502	reaction time	490:502	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	2	77	theme	C-dots	369:374	arg1	samples					376:382	C-dots samples	369:382	C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time	369:502	Ultra-high performance liquid chromatography (UHPLC) has been applied to study C-dots samples prepared with various synthetic conditions including amount of chitosan, concentration of acetic acid and reaction time.
26695273	10	78	theme	unique	1517:1522	arg1	characteristics					1524:1538	the unique characteristics	1513:1538	the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device	1513:1715	The findings highlight the virtues of UHPLC to separate and reveal the unique characteristics of individual C-dots product which may have potential applications in the fields of bioanalysis, bioimaging, catalysis, chemosensing, energy storage, and optoelectronics device.
26695273	0	79	theme	carbon	63:68	arg1	samples					75:81	as-synthesized carbon dots samples	48:81	as-synthesized carbon dots samples	48:81	UHPLC combined with mass spectrometric study of as-synthesized carbon dots samples.
26695273	6	80	theme	high-performance	904:919	arg1	chromatography					928:941	high-performance liquid chromatography	904:941	high-performance liquid chromatography	904:941	In addition, high-performance liquid chromatography is employed to fractionate the C-dots sample.
26695273	6	81	theme	C-dots	974:979	arg1	sample					981:986	the C-dots sample	970:986	the C-dots sample	970:986	In addition, high-performance liquid chromatography is employed to fractionate the C-dots sample.
26695273	3	82	theme	C-dots	564:569	arg1	species					571:577	C-dots species	564:577	C-dots species	564:577	All the as-prepared C-dots samples are complex mixtures of C-dots species which can be separated by UHPLC within 16 min.
26695273	1	83	theme	glacial	224:230	arg1	acid					239:242	glacial acetic acid	224:242	glacial acetic acid	224:242	A fast and green approach to synthesize carbon dots (C-dots) by microwave-assisted pyrolysis of precursor chitosan as the carbon source and glacial acetic acid as the condensation agent has been developed.
25526779	7	0	dep	mannosidases	1095:1106	arg1	proteins					1179:1186	degradation-enhancing alpha-mannosidase-like proteins	1134:1186	degradation-enhancing alpha-mannosidase-like proteins (EDEMs)	1134:1194	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	7	0	dep	mannosidases	1095:1106	arg1	EDEMs					1189:1193	EDEMs	1189:1193	EDEMs	1189:1193	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	4	1	theme	homologue	703:711	arg1	genes					713:717	seven homologue genes	697:717	seven homologue genes belonging to family 47 and one to family 63	697:761	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	4	2	theme	63	597:598	arg1	members					535:541	putative members	526:541	putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation	526:685	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	0	3	theme	glycosylation	90:102	arg1	pathway					104:110	the N-linked glycosylation pathway	77:110	the N-linked glycosylation pathway	77:110	Functional characterization of Sporothrix schenckii glycosidases involved in the N-linked glycosylation pathway.
25526779	4	4	theme	glycosyl	550:557	arg1	families					581:588	the glycosyl hydrolase (CAZy - GH) families 47 and 63	546:598	families	581:588	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	8	5	used	used	1370:1373	arg2	antifungals					1344:1354	antifungals	1344:1354	antifungals that could be used to treat sporotrichosis and other fungal diseases	1344:1423	Since these GH families gather proteins used as target for drugs to control cell growth, identification of these genes could help in the design of antifungals that could be used to treat sporotrichosis and other fungal diseases.
25526779	9	6	from	first	1468:1472	arg1	addition					1429:1436	addition	1429:1436	addition	1429:1436	In addition, to our knowledge, we are the first to report that Golgi mannosidases and EDEMs are expressed and characterized in yeast cells.
25526779	9	7	theme	yeast	1553:1557	arg1	cells					1559:1563	yeast cells	1553:1563	yeast cells	1553:1563	In addition, to our knowledge, we are the first to report that Golgi mannosidases and EDEMs are expressed and characterized in yeast cells.
25526779	2	8	theme	wall	326:329	arg1	composition					331:341	proper cell wall composition	314:341	proper cell wall composition	314:341	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	3	9	theme	causative	401:409	arg1	agent					411:415	the causative agent	397:415	the causative agent of sporotrichosis	397:433	In Sporothrix schenckii sensu stricto, the causative agent of sporotrichosis, little is known about this glycosylation pathway.
25526779	3	9	theme	causative	401:409	arg1	stricto					388:394	Sporothrix schenckii sensu stricto	361:394	Sporothrix schenckii sensu stricto	361:394	In Sporothrix schenckii sensu stricto, the causative agent of sporotrichosis, little is known about this glycosylation pathway.
25526779	7	10	theme	remaining	1053:1061	arg1	genes					1063:1067	The remaining genes	1049:1067	The remaining genes of family 47	1049:1080	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	7	10	theme	remaining	1053:1061	arg1	mannosidases					1095:1106	Golgi mannosidases	1089:1106	Golgi mannosidases	1089:1106	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	7	10	theme	remaining	1053:1061	arg1	reticulum					1124:1132	endoplasmic reticulum	1112:1132	endoplasmic reticulum	1112:1132	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	2	11	theme	cell	321:324	arg1	composition					331:341	proper cell wall composition	314:341	proper cell wall composition	314:341	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	7	12	theme	degradation-enhancing	1134:1154	arg1	proteins					1179:1186	degradation-enhancing alpha-mannosidase-like proteins	1134:1186	degradation-enhancing alpha-mannosidase-like proteins (EDEMs)	1134:1194	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	7	12	theme	degradation-enhancing	1134:1154	arg1	EDEMs					1189:1193	EDEMs	1189:1193	EDEMs	1189:1193	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	4	13	theme	genome-wide	500:510	arg1	screening					512:520	a genome-wide screening	498:520	a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation	498:685	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	6	14	theme	functional	1018:1027	arg1	ortholog					1029:1036	the functional ortholog	1014:1036	the functional ortholog of CaMNS1	1014:1046	Our results indicate that SsCWH41 is the functional ortholog of CaCWH41, whereas SsMNS1 is the functional ortholog of CaMNS1.
25526779	6	14	theme	functional	1018:1027	arg1	SsMNS1					1004:1009	SsMNS1	1004:1009	SsMNS1	1004:1009	Our results indicate that SsCWH41 is the functional ortholog of CaCWH41, whereas SsMNS1 is the functional ortholog of CaMNS1.
25526779	2	15	theme	proper	314:319	arg1	composition					331:341	proper cell wall composition	314:341	proper cell wall composition	314:341	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	2	16	from	pathogens	247:255	arg1	pathway					285:291	the N-linked glycosylation pathway	258:291	the N-linked glycosylation pathway	258:291	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	2	16	from	pathogens	247:255	arg1	indispensable					296:308	indispensable	296:308	indispensable	296:308	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	4	17	theme	glycan	669:674	arg1	maturation					676:685	N-linked glycan maturation	660:685	N-linked glycan maturation	660:685	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	7	18	theme	alpha-mannosidase-like	1156:1177	arg1	proteins					1179:1186	degradation-enhancing alpha-mannosidase-like proteins	1134:1186	degradation-enhancing alpha-mannosidase-like proteins (EDEMs)	1134:1194	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	7	18	theme	alpha-mannosidase-like	1156:1177	arg1	EDEMs					1189:1193	EDEMs	1189:1193	EDEMs	1189:1193	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	4	19	theme	putative	526:533	arg1	members					535:541	putative members	526:541	putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation	526:685	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	3	20	theme	schenckii	372:380	arg1	agent					411:415	the causative agent	397:415	the causative agent of sporotrichosis	397:433	In Sporothrix schenckii sensu stricto, the causative agent of sporotrichosis, little is known about this glycosylation pathway.
25526779	3	20	theme	schenckii	372:380	arg1	stricto					388:394	Sporothrix schenckii sensu stricto	361:394	Sporothrix schenckii sensu stricto	361:394	In Sporothrix schenckii sensu stricto, the causative agent of sporotrichosis, little is known about this glycosylation pathway.
25526779	6	21	theme	functional	964:973	arg1	SsCWH41					949:955	SsCWH41	949:955	SsCWH41	949:955	Our results indicate that SsCWH41 is the functional ortholog of CaCWH41, whereas SsMNS1 is the functional ortholog of CaMNS1.
25526779	6	21	theme	functional	964:973	arg1	ortholog					975:982	the functional ortholog	960:982	the functional ortholog of CaCWH41	960:993	Our results indicate that SsCWH41 is the functional ortholog of CaCWH41, whereas SsMNS1 is the functional ortholog of CaMNS1.
25526779	4	22	theme	N-linked	660:667	arg1	maturation					676:685	N-linked glycan maturation	660:685	N-linked glycan maturation	660:685	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	3	23	theme	sporotrichosis	420:433	arg1	agent					411:415	the causative agent	397:415	the causative agent of sporotrichosis	397:433	In Sporothrix schenckii sensu stricto, the causative agent of sporotrichosis, little is known about this glycosylation pathway.
25526779	3	23	theme	sporotrichosis	420:433	arg1	stricto					388:394	Sporothrix schenckii sensu stricto	361:394	Sporothrix schenckii sensu stricto	361:394	In Sporothrix schenckii sensu stricto, the causative agent of sporotrichosis, little is known about this glycosylation pathway.
25526779	3	24	theme	Sporothrix	361:370	arg1	schenckii					372:380	Sporothrix schenckii	361:380	Sporothrix schenckii sensu stricto	361:394	In Sporothrix schenckii sensu stricto, the causative agent of sporotrichosis, little is known about this glycosylation pathway.
25526779	1	25	from	processes	168:176	arg1	organisms					192:200	eukaryotic organisms	181:200	eukaryotic organisms	181:200	Protein glycosylation pathways are conserved metabolic processes in eukaryotic organisms and are required for cell fitness.
25526779	9	26	theme	Golgi	1489:1493	arg1	mannosidases					1495:1506	Golgi mannosidases	1489:1506	Golgi mannosidases	1489:1506	In addition, to our knowledge, we are the first to report that Golgi mannosidases and EDEMs are expressed and characterized in yeast cells.
25526779	4	27	dep	families	581:588	arg1	CAZy					570:573	CAZy	570:573	CAZy	570:573	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	4	27	dep	families	581:588	arg1	GH					577:578	GH	577:578	GH	577:578	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	0	28	theme	Functional	0:9	arg1	characterization					11:26	Functional characterization	0:26	Functional characterization of Sporothrix schenckii glycosidases	0:63	Functional characterization of Sporothrix schenckii glycosidases involved in the N-linked glycosylation pathway.
25526779	8	29	theme	GH	1209:1210	arg1	families					1212:1219	these GH families	1203:1219	these GH families	1203:1219	Since these GH families gather proteins used as target for drugs to control cell growth, identification of these genes could help in the design of antifungals that could be used to treat sporotrichosis and other fungal diseases.
25526779	1	30	theme	Protein	113:119	arg1	processes					168:176	conserved metabolic processes	148:176	conserved metabolic processes in eukaryotic organisms	148:200	Protein glycosylation pathways are conserved metabolic processes in eukaryotic organisms and are required for cell fitness.
25526779	1	30	theme	Protein	113:119	arg1	pathways					135:142	Protein glycosylation pathways	113:142	Protein glycosylation pathways	113:142	Protein glycosylation pathways are conserved metabolic processes in eukaryotic organisms and are required for cell fitness.
25526779	1	31	theme	cell	223:226	arg1	fitness					228:234	cell fitness	223:234	cell fitness	223:234	Protein glycosylation pathways are conserved metabolic processes in eukaryotic organisms and are required for cell fitness.
25526779	5	32	theme	C.	811:812	arg1	mutants					828:834	C. albicans null mutants	811:834	C. albicans null mutants lacking either MNS1 (for members of family 47) or CWH41 (for the member of family 63)	811:920	The eight genes were individually expressed in C. albicans null mutants lacking either MNS1 (for members of family 47) or CWH41 (for the member of family 63).
25526779	1	33	theme	glycosylation	121:133	arg1	processes					168:176	conserved metabolic processes	148:176	conserved metabolic processes in eukaryotic organisms	148:200	Protein glycosylation pathways are conserved metabolic processes in eukaryotic organisms and are required for cell fitness.
25526779	1	33	theme	glycosylation	121:133	arg1	pathways					135:142	Protein glycosylation pathways	113:142	Protein glycosylation pathways	113:142	Protein glycosylation pathways are conserved metabolic processes in eukaryotic organisms and are required for cell fitness.
25526779	8	34	theme	antifungals	1344:1354	arg1	design					1334:1339	the design	1330:1339	the design of antifungals that could be used to treat sporotrichosis and other fungal diseases	1330:1423	Since these GH families gather proteins used as target for drugs to control cell growth, identification of these genes could help in the design of antifungals that could be used to treat sporotrichosis and other fungal diseases.
25526779	4	35	theme	processing	637:646	arg1	step					648:651	the processing step	633:651	the processing step	633:651	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	5	36	theme	albicans	814:821	arg1	mutants					828:834	C. albicans null mutants	811:834	C. albicans null mutants lacking either MNS1 (for members of family 47) or CWH41 (for the member of family 63)	811:920	The eight genes were individually expressed in C. albicans null mutants lacking either MNS1 (for members of family 47) or CWH41 (for the member of family 63).
25526779	9	37	from	addition	1429:1436	arg1	we					1457:1458	we	1457:1458	we	1457:1458	In addition, to our knowledge, we are the first to report that Golgi mannosidases and EDEMs are expressed and characterized in yeast cells.
25526779	9	37	from	addition	1429:1436	arg1	first					1468:1472	first	1468:1472	first	1468:1472	In addition, to our knowledge, we are the first to report that Golgi mannosidases and EDEMs are expressed and characterized in yeast cells.
25526779	5	38	theme	family	911:916	arg1	member					901:906	the member	897:906	the member of family 63	897:919	The eight genes were individually expressed in C. albicans null mutants lacking either MNS1 (for members of family 47) or CWH41 (for the member of family 63).
25526779	2	39	from	indispensable	296:308	arg1	pathogens					247:255	fungal pathogens	240:255	fungal pathogens	240:255	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	0	40	theme	glycosidases	52:63	arg1	characterization					11:26	Functional characterization	0:26	Functional characterization of Sporothrix schenckii glycosidases	0:63	Functional characterization of Sporothrix schenckii glycosidases involved in the N-linked glycosylation pathway.
25526779	4	41	theme	families	581:588	arg1	members					535:541	putative members	526:541	putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation	526:685	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	2	42	theme	N-linked	262:269	arg1	pathway					285:291	the N-linked glycosylation pathway	258:291	the N-linked glycosylation pathway	258:291	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	2	42	theme	N-linked	262:269	arg1	indispensable					296:308	indispensable	296:308	indispensable	296:308	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	3	43	theme	glycosylation	463:475	arg1	pathway					477:483	this glycosylation pathway	458:483	this glycosylation pathway	458:483	In Sporothrix schenckii sensu stricto, the causative agent of sporotrichosis, little is known about this glycosylation pathway.
25526779	6	44	theme	CaCWH41	987:993	arg1	SsCWH41					949:955	SsCWH41	949:955	SsCWH41	949:955	Our results indicate that SsCWH41 is the functional ortholog of CaCWH41, whereas SsMNS1 is the functional ortholog of CaMNS1.
25526779	6	44	theme	CaCWH41	987:993	arg1	ortholog					975:982	the functional ortholog	960:982	the functional ortholog of CaCWH41	960:993	Our results indicate that SsCWH41 is the functional ortholog of CaCWH41, whereas SsMNS1 is the functional ortholog of CaMNS1.
25526779	0	45	theme	schenckii	42:50	arg1	glycosidases					52:63	Sporothrix schenckii glycosidases	31:63	Sporothrix schenckii glycosidases	31:63	Functional characterization of Sporothrix schenckii glycosidases involved in the N-linked glycosylation pathway.
25526779	7	46	theme	Golgi	1089:1093	arg1	genes					1063:1067	The remaining genes	1049:1067	The remaining genes of family 47	1049:1080	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	7	46	theme	Golgi	1089:1093	arg1	mannosidases					1095:1106	Golgi mannosidases	1089:1106	Golgi mannosidases	1089:1106	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	8	47	theme	fungal	1409:1414	arg1	diseases					1416:1423	other fungal diseases	1403:1423	other fungal diseases	1403:1423	Since these GH families gather proteins used as target for drugs to control cell growth, identification of these genes could help in the design of antifungals that could be used to treat sporotrichosis and other fungal diseases.
25526779	7	48	theme	family	1072:1077	arg1	genes					1063:1067	The remaining genes	1049:1067	The remaining genes of family 47	1049:1080	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	7	48	theme	family	1072:1077	arg1	mannosidases					1095:1106	Golgi mannosidases	1089:1106	Golgi mannosidases	1089:1106	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	7	48	theme	family	1072:1077	arg1	reticulum					1124:1132	endoplasmic reticulum	1112:1132	endoplasmic reticulum	1112:1132	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	4	49	theme	hydrolase	559:567	arg1	families					581:588	the glycosyl hydrolase (CAZy - GH) families 47 and 63	546:598	families	581:588	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	8	50	theme	other	1403:1407	arg1	diseases					1416:1423	other fungal diseases	1403:1423	other fungal diseases	1403:1423	Since these GH families gather proteins used as target for drugs to control cell growth, identification of these genes could help in the design of antifungals that could be used to treat sporotrichosis and other fungal diseases.
25526779	6	51	dep	ortholog	975:982	arg1	whereas					996:1002	whereas	996:1002	whereas	996:1002	Our results indicate that SsCWH41 is the functional ortholog of CaCWH41, whereas SsMNS1 is the functional ortholog of CaMNS1.
25526779	8	52	theme	cell	1273:1276	arg1	growth					1278:1283	cell growth	1273:1283	cell growth	1273:1283	Since these GH families gather proteins used as target for drugs to control cell growth, identification of these genes could help in the design of antifungals that could be used to treat sporotrichosis and other fungal diseases.
25526779	2	53	theme	fungal	240:245	arg1	pathogens					247:255	fungal pathogens	240:255	fungal pathogens	240:255	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	4	54	link	N-linked	660:667	arg1	maturation					676:685	N-linked glycan maturation	660:685	N-linked glycan maturation	660:685	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	0	55	link	N-linked	81:88	arg1	pathway					104:110	the N-linked glycosylation pathway	77:110	the N-linked glycosylation pathway	77:110	Functional characterization of Sporothrix schenckii glycosidases involved in the N-linked glycosylation pathway.
25526779	1	56	theme	conserved	148:156	arg1	processes					168:176	conserved metabolic processes	148:176	conserved metabolic processes in eukaryotic organisms	148:200	Protein glycosylation pathways are conserved metabolic processes in eukaryotic organisms and are required for cell fitness.
25526779	1	56	theme	conserved	148:156	arg1	pathways					135:142	Protein glycosylation pathways	113:142	Protein glycosylation pathways	113:142	Protein glycosylation pathways are conserved metabolic processes in eukaryotic organisms and are required for cell fitness.
25526779	1	57	theme	metabolic	158:166	arg1	processes					168:176	conserved metabolic processes	148:176	conserved metabolic processes in eukaryotic organisms	148:200	Protein glycosylation pathways are conserved metabolic processes in eukaryotic organisms and are required for cell fitness.
25526779	1	57	theme	metabolic	158:166	arg1	pathways					135:142	Protein glycosylation pathways	113:142	Protein glycosylation pathways	113:142	Protein glycosylation pathways are conserved metabolic processes in eukaryotic organisms and are required for cell fitness.
25526779	8	58	theme	genes	1310:1314	arg1	identification					1286:1299	identification	1286:1299	identification of these genes	1286:1314	Since these GH families gather proteins used as target for drugs to control cell growth, identification of these genes could help in the design of antifungals that could be used to treat sporotrichosis and other fungal diseases.
25526779	7	59	theme	endoplasmic	1112:1122	arg1	genes					1063:1067	The remaining genes	1049:1067	The remaining genes of family 47	1049:1080	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	7	59	theme	endoplasmic	1112:1122	arg1	reticulum					1124:1132	endoplasmic reticulum	1112:1132	endoplasmic reticulum	1112:1132	The remaining genes of family 47 encode Golgi mannosidases and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like proteins (EDEMs).
25526779	5	60	theme	family	872:877	arg1	members					861:867	members	861:867	members of family 47	861:880	The eight genes were individually expressed in C. albicans null mutants lacking either MNS1 (for members of family 47) or CWH41 (for the member of family 63).
25526779	4	61	theme	group	607:611	arg1	enzymes					613:619	enzymes	613:619	enzymes	613:619	Here, using a genome-wide screening for putative members of the glycosyl hydrolase (CAZy - GH) families 47 and 63, which group enzymes involved in the processing step during N-linked glycan maturation, we found seven homologue genes belonging to family 47 and one to family 63.
25526779	0	62	theme	N-linked	81:88	arg1	pathway					104:110	the N-linked glycosylation pathway	77:110	the N-linked glycosylation pathway	77:110	Functional characterization of Sporothrix schenckii glycosidases involved in the N-linked glycosylation pathway.
25526779	6	63	theme	CaMNS1	1041:1046	arg1	ortholog					1029:1036	the functional ortholog	1014:1036	the functional ortholog of CaMNS1	1014:1046	Our results indicate that SsCWH41 is the functional ortholog of CaCWH41, whereas SsMNS1 is the functional ortholog of CaMNS1.
25526779	6	63	theme	CaMNS1	1041:1046	arg1	SsMNS1					1004:1009	SsMNS1	1004:1009	SsMNS1	1004:1009	Our results indicate that SsCWH41 is the functional ortholog of CaCWH41, whereas SsMNS1 is the functional ortholog of CaMNS1.
25526779	5	64	theme	null	823:826	arg1	mutants					828:834	C. albicans null mutants	811:834	C. albicans null mutants lacking either MNS1 (for members of family 47) or CWH41 (for the member of family 63)	811:920	The eight genes were individually expressed in C. albicans null mutants lacking either MNS1 (for members of family 47) or CWH41 (for the member of family 63).
25526779	2	65	link	N-linked	262:269	arg1	pathway					285:291	the N-linked glycosylation pathway	258:291	the N-linked glycosylation pathway	258:291	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	2	65	link	N-linked	262:269	arg1	indispensable					296:308	indispensable	296:308	indispensable	296:308	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	3	66	theme	sensu	382:386	arg1	agent					411:415	the causative agent	397:415	the causative agent of sporotrichosis	397:433	In Sporothrix schenckii sensu stricto, the causative agent of sporotrichosis, little is known about this glycosylation pathway.
25526779	3	66	theme	sensu	382:386	arg1	stricto					388:394	Sporothrix schenckii sensu stricto	361:394	Sporothrix schenckii sensu stricto	361:394	In Sporothrix schenckii sensu stricto, the causative agent of sporotrichosis, little is known about this glycosylation pathway.
25526779	2	67	theme	glycosylation	271:283	arg1	pathway					285:291	the N-linked glycosylation pathway	258:291	the N-linked glycosylation pathway	258:291	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	2	67	theme	glycosylation	271:283	arg1	indispensable					296:308	indispensable	296:308	indispensable	296:308	In fungal pathogens, the N-linked glycosylation pathway is indispensable for proper cell wall composition and virulence.
25526779	1	68	theme	eukaryotic	181:190	arg1	organisms					192:200	eukaryotic organisms	181:200	eukaryotic organisms	181:200	Protein glycosylation pathways are conserved metabolic processes in eukaryotic organisms and are required for cell fitness.
29181012	9	0	theme	transient	1629:1637	arg1	desiccation					1639:1649	transient desiccation	1629:1649	transient desiccation	1629:1649	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	2	1	from	plants	418:423	arg1	present					351:357	present	351:357	present	351:357	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	10	2	theme	wall	1889:1892	arg1	polysaccharides					1894:1908	cell wall polysaccharides	1884:1908	cell wall polysaccharides	1884:1908	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	10	3	theme	cell	1884:1887	arg1	polysaccharides					1894:1908	cell wall polysaccharides	1884:1908	cell wall polysaccharides	1884:1908	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	3	4	theme	green	496:500	arg1	algae					502:506	green algae	496:506	green algae	496:506	Cladophora (Ulvophyceae, Chlorophyta) is one of the largest genera of green algae that are able to grow in both, seas and freshwater courses.
29181012	1	5	theme	high	252:255	arg1	salinity					257:264	the high salinity	248:264	the high salinity of the marine environment	248:290	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	10	6	from	mechanisms	1857:1866	arg1	polysaccharides					1894:1908	cell wall polysaccharides	1884:1908	cell wall polysaccharides	1884:1908	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	10	6	from	mechanisms	1857:1866	arg1	co-evolution					1935:1946	environmental stress co-evolution	1914:1946	environmental stress co-evolution	1914:1946	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	2	7	attach	present	351:357	arg1	species					392:398	certain freshwater Cladophora species	362:398	certain freshwater Cladophora species	362:398	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	2	7	attach	present	351:357	arg1	plants					418:423	some vascular plants	404:423	some vascular plants	404:423	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	2	7	attach	present	351:357	arg2	polysaccharides					330:344	sulfated polysaccharides	321:344	sulfated polysaccharides	321:344	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	10	8	from	sulfation	1871:1879	arg1	polysaccharides					1894:1908	cell wall polysaccharides	1884:1908	cell wall polysaccharides	1884:1908	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	10	8	from	sulfation	1871:1879	arg1	co-evolution					1935:1946	environmental stress co-evolution	1914:1946	environmental stress co-evolution	1914:1946	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	6	9	theme	Cladophora	1159:1168	arg1	surera					1170:1175	the freshwater alga Cladophora surera	1139:1175	the freshwater alga Cladophora surera	1139:1175	In this work, the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera were characterized.
29181012	10	10	theme	environmental	1914:1926	arg1	co-evolution					1935:1946	environmental stress co-evolution	1914:1946	environmental stress co-evolution	1914:1946	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	9	11	from	presence	1507:1514	arg1	surera					1581:1586	the freshwater green macroalga C. surera	1547:1586	the freshwater green macroalga C. surera	1547:1586	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	6	12	theme	freshwater	1143:1152	arg1	surera					1170:1175	the freshwater alga Cladophora surera	1139:1175	the freshwater alga Cladophora surera	1139:1175	In this work, the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera were characterized.
29181012	4	13	theme	species	648:654	arg1	polysaccharides					618:632	the water-soluble polysaccharides	600:632	the water-soluble polysaccharides of the marine species C. falklandica	600:669	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	10	14	theme	evolutionary	1769:1780	arg1	event					1782:1786	an evolutionary event	1766:1786	an evolutionary event	1766:1786	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	10	15	from	polysaccharides	1894:1908	arg1	mechanisms					1857:1866	the mechanisms	1853:1866	the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution	1853:1946	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	4	16	theme	β-D-galactofuranose	916:934	arg1	units					936:940	terminal β-D-xylopyranose or β-D-galactofuranose units	887:940	terminal β-D-xylopyranose or β-D-galactofuranose units	887:940	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	7	17	located	found	1306:1310	arg1	species					1322:1328	marine species	1315:1328	marine species of this genus	1315:1342	It was found that this green alga biosynthesizes sulfated polysaccharides, with a structure similar to those found in marine species of this genus.
29181012	7	17	located	found	1306:1310	arg2	those					1300:1304	those	1300:1304	those	1300:1304	It was found that this green alga biosynthesizes sulfated polysaccharides, with a structure similar to those found in marine species of this genus.
29181012	4	18	gly	glycosylation	853:865	arg1	C2					837:838	C2	837:838	C2 with partial glycosylation	837:865	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	10	19	from	co-evolution	1935:1946	arg1	mechanisms					1857:1866	the mechanisms	1853:1866	the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution	1853:1946	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	9	20	from	changes	1655:1661	arg1	case					1606:1609	this case	1601:1609	this case	1601:1609	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	9	20	from	changes	1655:1661	arg1	strength					1672:1679	ionic strength	1666:1679	ionic strength	1666:1679	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	10	21	theme	polysaccharides	1704:1718	arg1	Retention					1682:1690	Retention	1682:1690	Retention of sulfated polysaccharides at the cell walls	1682:1736	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	3	22	theme	freshwater	548:557	arg1	courses					559:565	freshwater courses	548:565	freshwater courses	548:565	Cladophora (Ulvophyceae, Chlorophyta) is one of the largest genera of green algae that are able to grow in both, seas and freshwater courses.
29181012	4	23	theme	β-D-xylopyranose	896:911	arg1	units					936:940	terminal β-D-xylopyranose or β-D-galactofuranose units	887:940	terminal β-D-xylopyranose or β-D-galactofuranose units	887:940	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	4	24	theme	xylogalactoarabinans	708:727	arg1	presence					687:694	the presence	683:694	the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2	683:879	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	8	25	with	Calibration	1345:1355	arg1	data					1388:1391	fossil data	1381:1391	fossil data	1381:1391	Calibration of molecular clock with fossil data suggests that colonization of freshwater environments occurred during the Miocene by its ancestor.
29181012	10	26	theme	excellent	1814:1822	arg1	model					1824:1828	an excellent model	1811:1828	an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution	1811:1946	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	1	27	theme	polysaccharides	137:151	arg1	presence					116:123	The presence	112:123	The presence of sulfated polysaccharides in cell walls of seaweeds	112:177	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	1	27	theme	polysaccharides	137:151	arg1	consequence					201:211	a consequence	199:211	a consequence of the physiological adaptation to the high salinity of the marine environment	199:290	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	7	28	theme	green	1220:1224	arg1	alga					1226:1229	this green alga	1215:1229	this green alga	1215:1229	It was found that this green alga biosynthesizes sulfated polysaccharides, with a structure similar to those found in marine species of this genus.
29181012	1	29	theme	cell	156:159	arg1	walls					161:165	cell walls	156:165	cell walls of seaweeds	156:177	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	2	30	located	present	351:357	arg1	species					392:398	certain freshwater Cladophora species	362:398	certain freshwater Cladophora species	362:398	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	2	30	located	present	351:357	arg2	polysaccharides					330:344	sulfated polysaccharides	321:344	sulfated polysaccharides	321:344	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	2	30	located	present	351:357	arg1	plants					418:423	some vascular plants	404:423	some vascular plants	404:423	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	4	31	theme	4-linked	758:765	arg1	units					787:791	4-linked β-L-arabinopyranose units	758:791	4-linked β-L-arabinopyranose units partially sulfated	758:810	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	0	32	theme	Salinity	91:98	arg1	Adaptation					100:109	Salinity Adaptation	91:109	Salinity Adaptation	91:109	Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation.
29181012	5	33	theme	3-	969:970	arg1	3-					969:970	3-	969:970	3-	969:970	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	33	theme	3-	969:970	arg1	amounts					958:964	minor amounts	952:964	minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form	952:1054	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	33	theme	3-	969:970	arg1	structures					1007:1016	3,6-linked β-D-galactan structures	983:1016	3,6-linked β-D-galactan structures	983:1016	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	33	theme	3-	969:970	arg1	6-					973:974	6-	973:974	6-	973:974	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	9	34	from	adaptation	1615:1624	arg1	case					1606:1609	this case	1601:1609	this case	1601:1609	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	9	34	from	adaptation	1615:1624	arg1	strength					1672:1679	ionic strength	1666:1679	ionic strength	1666:1679	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	4	35	theme	units	787:791	arg1	backbone					746:753	a backbone	744:753	a backbone of 4-linked β-L-arabinopyranose units partially sulfated	744:810	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	5	36	theme	6-	973:974	arg1	3-					969:970	3-	969:970	3-	969:970	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	36	theme	6-	973:974	arg1	amounts					958:964	minor amounts	952:964	minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form	952:1054	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	36	theme	6-	973:974	arg1	structures					1007:1016	3,6-linked β-D-galactan structures	983:1016	3,6-linked β-D-galactan structures	983:1016	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	36	theme	6-	973:974	arg1	6-					973:974	6-	973:974	6-	973:974	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	8	37	theme	molecular	1360:1368	arg1	clock					1370:1374	molecular clock	1360:1374	molecular clock	1360:1374	Calibration of molecular clock with fossil data suggests that colonization of freshwater environments occurred during the Miocene by its ancestor.
29181012	6	38	from	surera	1170:1175	arg1	polysaccharides					1118:1132	the main water soluble cell wall polysaccharides	1085:1132	the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera	1085:1175	In this work, the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera were characterized.
29181012	2	39	theme	Cladophora	381:390	arg1	species					392:398	certain freshwater Cladophora species	362:398	certain freshwater Cladophora species	362:398	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	0	40	theme	Sulfated	0:7	arg1	Polysaccharides					9:23	Sulfated Polysaccharides	0:23	Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera	0:75	Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation.
29181012	1	41	from	presence	116:123	arg1	walls					161:165	cell walls	156:165	cell walls of seaweeds	156:177	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	7	42	theme	marine	1315:1320	arg1	species					1322:1328	marine species	1315:1328	marine species of this genus	1315:1342	It was found that this green alga biosynthesizes sulfated polysaccharides, with a structure similar to those found in marine species of this genus.
29181012	1	43	theme	environment	280:290	arg1	salinity					257:264	the high salinity	248:264	the high salinity of the marine environment	248:290	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	9	44	attach	presence	1507:1514	arg1	surera					1581:1586	the freshwater green macroalga C. surera	1547:1586	the freshwater green macroalga C. surera	1547:1586	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	9	44	attach	presence	1507:1514	arg2	polysaccharides					1528:1542	sulfated polysaccharides	1519:1542	sulfated polysaccharides	1519:1542	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	2	45	theme	vascular	409:416	arg1	plants					418:423	some vascular plants	404:423	some vascular plants	404:423	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	0	46	theme	Freshwater	32:41	arg1	surera					70:75	the Freshwater Green Macroalga Cladophora surera	28:75	the Freshwater Green Macroalga Cladophora surera	28:75	Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation.
29181012	3	47	theme	largest	478:484	arg1	genera					486:491	the largest genera	474:491	the largest genera of green algae	474:506	Cladophora (Ulvophyceae, Chlorophyta) is one of the largest genera of green algae that are able to grow in both, seas and freshwater courses.
29181012	0	48	theme	Macroalga	49:57	arg1	surera					70:75	the Freshwater Green Macroalga Cladophora surera	28:75	the Freshwater Green Macroalga Cladophora surera	28:75	Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation.
29181012	0	49	attach	Linked	81:86	arg1	Adaptation					100:109	Salinity Adaptation	91:109	Salinity Adaptation	91:109	Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation.
29181012	0	49	attach	Linked	81:86	arg2	Polysaccharides					9:23	Sulfated Polysaccharides	0:23	Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera	0:75	Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation.
29181012	6	50	theme	cell	1108:1111	arg1	polysaccharides					1118:1132	the main water soluble cell wall polysaccharides	1085:1132	the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera	1085:1175	In this work, the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera were characterized.
29181012	4	51	theme	sulfated	803:810	arg1	units					787:791	4-linked β-L-arabinopyranose units	758:791	4-linked β-L-arabinopyranose units partially sulfated	758:810	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	5	52	from	amounts	958:964	arg1	form					1051:1054	the pyranosic form	1037:1054	the pyranosic form	1037:1054	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	53	theme	3,6-linked	983:992	arg1	structures					1007:1016	3,6-linked β-D-galactan structures	983:1016	3,6-linked β-D-galactan structures	983:1016	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	6	54	theme	water	1094:1098	arg1	polysaccharides					1118:1132	the main water soluble cell wall polysaccharides	1085:1132	the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera	1085:1175	In this work, the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera were characterized.
29181012	4	55	theme	Previous	568:575	arg1	studies					577:583	Previous studies	568:583	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica	568:669	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	10	56	theme	sulfated	1695:1702	arg1	polysaccharides					1704:1718	sulfated polysaccharides	1695:1718	sulfated polysaccharides	1695:1718	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	8	57	theme	environments	1434:1445	arg1	colonization					1407:1418	colonization	1407:1418	colonization of freshwater environments	1407:1445	Calibration of molecular clock with fossil data suggests that colonization of freshwater environments occurred during the Miocene by its ancestor.
29181012	3	58	theme	algae	502:506	arg1	genera					486:491	the largest genera	474:491	the largest genera of green algae	474:506	Cladophora (Ulvophyceae, Chlorophyta) is one of the largest genera of green algae that are able to grow in both, seas and freshwater courses.
29181012	4	59	from	presence	687:694	arg1	C2					837:838	C2	837:838	C2 with partial glycosylation	837:865	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	4	59	from	presence	687:694	arg1	C3					822:823	C3	822:823	C3	822:823	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	4	59	from	presence	687:694	arg1	C2					878:879	C2	878:879	C2	878:879	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	1	60	theme	adaptation	234:243	arg1	presence					116:123	The presence	112:123	The presence of sulfated polysaccharides in cell walls of seaweeds	112:177	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	1	60	theme	adaptation	234:243	arg1	consequence					201:211	a consequence	199:211	a consequence of the physiological adaptation to the high salinity of the marine environment	199:290	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	5	61	theme	structures	1007:1016	arg1	3-					969:970	3-	969:970	3-	969:970	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	61	theme	structures	1007:1016	arg1	amounts					958:964	minor amounts	952:964	minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form	952:1054	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	61	theme	structures	1007:1016	arg1	structures					1007:1016	3,6-linked β-D-galactan structures	983:1016	3,6-linked β-D-galactan structures	983:1016	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	61	theme	structures	1007:1016	arg1	6-					973:974	6-	973:974	6-	973:974	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	1	62	attach	presence	116:123	arg1	walls					161:165	cell walls	156:165	cell walls of seaweeds	156:177	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	1	62	attach	presence	116:123	arg2	polysaccharides					137:151	sulfated polysaccharides	128:151	sulfated polysaccharides	128:151	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	10	63	from	studies	1842:1848	arg1	mechanisms					1857:1866	the mechanisms	1853:1866	the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution	1853:1946	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	10	64	theme	stress	1928:1933	arg1	co-evolution					1935:1946	environmental stress co-evolution	1914:1946	environmental stress co-evolution	1914:1946	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	1	65	theme	seaweeds	170:177	arg1	walls					161:165	cell walls	156:165	cell walls of seaweeds	156:177	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	9	66	theme	ionic	1666:1670	arg1	strength					1672:1679	ionic strength	1666:1679	ionic strength	1666:1679	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	2	67	from	present	351:357	arg1	species					392:398	certain freshwater Cladophora species	362:398	certain freshwater Cladophora species	362:398	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	2	67	from	present	351:357	arg1	plants					418:423	some vascular plants	404:423	some vascular plants	404:423	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	6	68	theme	alga	1154:1157	arg1	surera					1170:1175	the freshwater alga Cladophora surera	1139:1175	the freshwater alga Cladophora surera	1139:1175	In this work, the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera were characterized.
29181012	10	69	theme	event	1782:1786	arg1	snapshot					1754:1761	a snapshot	1752:1761	a snapshot of an evolutionary event	1752:1786	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	4	70	theme	partial	845:851	arg1	glycosylation					853:865	partial glycosylation	845:865	partial glycosylation	845:865	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	10	71	from	walls	1732:1736	arg1	Retention					1682:1690	Retention	1682:1690	Retention of sulfated polysaccharides at the cell walls	1682:1736	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	4	72	theme	marine	641:646	arg1	species					648:654	the marine species	637:654	the marine species C. falklandica	637:669	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	5	73	link	3,6-linked	983:992	arg1	structures					1007:1016	3,6-linked β-D-galactan structures	983:1016	3,6-linked β-D-galactan structures	983:1016	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	2	74	theme	certain	362:368	arg1	species					392:398	certain freshwater Cladophora species	362:398	certain freshwater Cladophora species	362:398	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	2	75	theme	sulfated	321:328	arg1	polysaccharides					330:344	sulfated polysaccharides	321:344	sulfated polysaccharides	321:344	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	4	76	theme	water-soluble	604:616	arg1	polysaccharides					618:632	the water-soluble polysaccharides	600:632	the water-soluble polysaccharides of the marine species C. falklandica	600:669	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	9	77	theme	sulfated	1519:1526	arg1	polysaccharides					1528:1542	sulfated polysaccharides	1519:1542	sulfated polysaccharides	1519:1542	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	5	78	theme	pyranosic	1041:1049	arg1	form					1051:1054	the pyranosic form	1037:1054	the pyranosic form	1037:1054	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	1	79	theme	sulfated	128:135	arg1	polysaccharides					137:151	sulfated polysaccharides	128:151	sulfated polysaccharides	128:151	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	4	80	theme	terminal	887:894	arg1	units					936:940	terminal β-D-xylopyranose or β-D-galactofuranose units	887:940	terminal β-D-xylopyranose or β-D-galactofuranose units	887:940	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	4	81	theme	sulfated	699:706	arg1	xylogalactoarabinans					708:727	sulfated xylogalactoarabinans	699:727	sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated	699:810	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	4	82	link	4-linked	758:765	arg1	units					787:791	4-linked β-L-arabinopyranose units	758:791	4-linked β-L-arabinopyranose units partially sulfated	758:810	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	7	83	theme	genus	1338:1342	arg1	species					1322:1328	marine species	1315:1328	marine species of this genus	1315:1342	It was found that this green alga biosynthesizes sulfated polysaccharides, with a structure similar to those found in marine species of this genus.
29181012	4	84	theme	C.	656:657	arg1	species					648:654	the marine species	637:654	the marine species C. falklandica	637:669	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	9	85	theme	polysaccharides	1528:1542	arg1	presence					1507:1514	the presence	1503:1514	the presence of sulfated polysaccharides in the freshwater green macroalga C. surera	1503:1586	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	9	85	theme	polysaccharides	1528:1542	arg1	adaptation					1615:1624	an adaptation	1612:1624	an adaptation to transient desiccation	1612:1649	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	0	86	from	Polysaccharides	9:23	arg1	surera					70:75	the Freshwater Green Macroalga Cladophora surera	28:75	the Freshwater Green Macroalga Cladophora surera	28:75	Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation.
29181012	4	87	with	C2	837:838	arg1	glycosylation					853:865	partial glycosylation	845:865	partial glycosylation	845:865	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	9	88	theme	freshwater	1551:1560	arg1	surera					1581:1586	the freshwater green macroalga C. surera	1547:1586	the freshwater green macroalga C. surera	1547:1586	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	9	89	theme	green	1562:1566	arg1	surera					1581:1586	the freshwater green macroalga C. surera	1547:1586	the freshwater green macroalga C. surera	1547:1586	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	4	90	theme	β-L-arabinopyranose	767:785	arg1	units					787:791	4-linked β-L-arabinopyranose units	758:791	4-linked β-L-arabinopyranose units partially sulfated	758:810	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	9	91	theme	macroalga	1568:1576	arg1	surera					1581:1586	the freshwater green macroalga C. surera	1547:1586	the freshwater green macroalga C. surera	1547:1586	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	9	92	theme	C.	1578:1579	arg1	surera					1581:1586	the freshwater green macroalga C. surera	1547:1586	the freshwater green macroalga C. surera	1547:1586	Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength.
29181012	2	93	theme	freshwater	370:379	arg1	species					392:398	certain freshwater Cladophora species	362:398	certain freshwater Cladophora species	362:398	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	1	94	theme	marine	273:278	arg1	environment					280:290	the marine environment	269:290	the marine environment	269:290	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	5	95	theme	minor	952:956	arg1	3-					969:970	3-	969:970	3-	969:970	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	95	theme	minor	952:956	arg1	amounts					958:964	minor amounts	952:964	minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form	952:1054	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	95	theme	minor	952:956	arg1	structures					1007:1016	3,6-linked β-D-galactan structures	983:1016	3,6-linked β-D-galactan structures	983:1016	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	5	95	theme	minor	952:956	arg1	6-					973:974	6-	973:974	6-	973:974	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	8	96	theme	clock	1370:1374	arg1	Calibration					1345:1355	Calibration	1345:1355	Calibration of molecular clock with fossil data	1345:1391	Calibration of molecular clock with fossil data suggests that colonization of freshwater environments occurred during the Miocene by its ancestor.
29181012	6	97	theme	main	1089:1092	arg1	polysaccharides					1118:1132	the main water soluble cell wall polysaccharides	1085:1132	the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera	1085:1175	In this work, the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera were characterized.
29181012	0	98	theme	Green	43:47	arg1	surera					70:75	the Freshwater Green Macroalga Cladophora surera	28:75	the Freshwater Green Macroalga Cladophora surera	28:75	Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation.
29181012	3	99	dep	Cladophora	426:435	arg1	Ulvophyceae					438:448	Ulvophyceae	438:448	Ulvophyceae	438:448	Cladophora (Ulvophyceae, Chlorophyta) is one of the largest genera of green algae that are able to grow in both, seas and freshwater courses.
29181012	3	99	dep	Cladophora	426:435	arg1	Chlorophyta					451:461	Chlorophyta	451:461	Chlorophyta	451:461	Cladophora (Ulvophyceae, Chlorophyta) is one of the largest genera of green algae that are able to grow in both, seas and freshwater courses.
29181012	7	100	theme	similar	1289:1295	arg1	structure					1279:1287	a structure	1277:1287	a structure similar to those found in marine species of this genus	1277:1342	It was found that this green alga biosynthesizes sulfated polysaccharides, with a structure similar to those found in marine species of this genus.
29181012	10	101	theme	further	1834:1840	arg1	studies					1842:1848	further studies	1834:1848	further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution	1834:1946	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	8	102	theme	fossil	1381:1386	arg1	data					1388:1391	fossil data	1381:1391	fossil data	1381:1391	Calibration of molecular clock with fossil data suggests that colonization of freshwater environments occurred during the Miocene by its ancestor.
29181012	0	103	theme	Cladophora	59:68	arg1	surera					70:75	the Freshwater Green Macroalga Cladophora surera	28:75	the Freshwater Green Macroalga Cladophora surera	28:75	Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation.
29181012	6	104	theme	wall	1113:1116	arg1	polysaccharides					1118:1132	the main water soluble cell wall polysaccharides	1085:1132	the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera	1085:1175	In this work, the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera were characterized.
29181012	10	105	theme	sulfation	1871:1879	arg1	mechanisms					1857:1866	the mechanisms	1853:1866	the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution	1853:1946	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29181012	3	106	theme	genera	486:491	arg1	genera					486:491	the largest genera	474:491	the largest genera of green algae	474:506	Cladophora (Ulvophyceae, Chlorophyta) is one of the largest genera of green algae that are able to grow in both, seas and freshwater courses.
29181012	3	106	theme	genera	486:491	arg1	one					467:469	one	467:469	one	467:469	Cladophora (Ulvophyceae, Chlorophyta) is one of the largest genera of green algae that are able to grow in both, seas and freshwater courses.
29181012	2	107	from	species	392:398	arg1	present					351:357	present	351:357	present	351:357	Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants.
29181012	6	108	theme	soluble	1100:1106	arg1	polysaccharides					1118:1132	the main water soluble cell wall polysaccharides	1085:1132	the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera	1085:1175	In this work, the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera were characterized.
29181012	4	109	dep	C.	656:657	arg1	falklandica					659:669	falklandica	659:669	falklandica	659:669	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	1	110	theme	physiological	220:232	arg1	adaptation					234:243	the physiological adaptation	216:243	the physiological adaptation to the high salinity of the marine environment	216:290	The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment.
29181012	5	111	theme	β-D-galactan	994:1005	arg1	structures					1007:1016	3,6-linked β-D-galactan structures	983:1016	3,6-linked β-D-galactan structures	983:1016	Besides, minor amounts of 3-, 6- and/or 3,6-linked β-D-galactan structures, with galactose in the pyranosic form were detected.
29181012	7	112	theme	sulfated	1246:1253	arg1	polysaccharides					1255:1269	sulfated polysaccharides	1246:1269	sulfated polysaccharides	1246:1269	It was found that this green alga biosynthesizes sulfated polysaccharides, with a structure similar to those found in marine species of this genus.
29181012	4	113	attach	presence	687:694	arg2	xylogalactoarabinans					708:727	sulfated xylogalactoarabinans	699:727	sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated	699:810	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	4	113	attach	presence	687:694	arg1	C2					837:838	C2	837:838	C2 with partial glycosylation	837:865	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	4	113	attach	presence	687:694	arg1	C3					822:823	C3	822:823	C3	822:823	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	4	113	attach	presence	687:694	arg1	C2					878:879	C2	878:879	C2	878:879	Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal β-D-xylopyranose or β-D-galactofuranose units.
29181012	8	114	theme	freshwater	1423:1432	arg1	environments					1434:1445	freshwater environments	1423:1445	freshwater environments	1423:1445	Calibration of molecular clock with fossil data suggests that colonization of freshwater environments occurred during the Miocene by its ancestor.
29181012	10	115	theme	cell	1727:1730	arg1	walls					1732:1736	the cell walls	1723:1736	the cell walls	1723:1736	Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
29253942	0	0	theme	cellulose	80:88	arg1	nanofibrils					90:100	cellulose nanofibrils	80:100	cellulose nanofibrils	80:100	Enzymatic pretreatment for the improvement of dispersion and film properties of cellulose nanofibrils.
29253942	8	1	theme	CNF	1141:1143	arg1	distribution					1125:1136	the even distribution	1116:1136	the even distribution of CNF in water	1116:1152	The carboxyl group content increased after the enzymatic pretreatment, which benefits the even distribution of CNF in water.
29253942	11	2	theme	continuous	1520:1529	arg1	production					1544:1553	the continuous roll to roll production	1516:1553	the continuous roll to roll production of CNF film	1516:1565	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	1	3	theme	treatment	228:236	arg1	effect					208:213	the effect	204:213	the effect of enzymatic treatment on the properties of CNF	204:261	In the present study, cellulose nanofibrils (CNF) were produced from unbleached eucalyptus pulp, and the effect of enzymatic treatment on the properties of CNF was studied.
29253942	5	4	theme	vacuum	764:769	arg1	process					782:788	a vacuum filtration process	762:788	a vacuum filtration process	762:788	CNF films were produced with a vacuum filtration process.
29253942	6	5	theme	strength	818:825	arg1	properties					827:836	The optical and mechanical strength properties	791:836	The optical and mechanical strength properties of the CNF films	791:853	The optical and mechanical strength properties of the CNF films were improved by the removal of hemicellulose.
29253942	3	6	theme	high-pressure	486:498	arg1	homogenization					500:513	high-pressure homogenization	486:513	high-pressure homogenization	486:513	The xylanase pretreatment facilitated the deconstruction of pulp into CNF via mechanical shearing and high-pressure homogenization, while the hemicellulose composition was preserved.
29253942	9	7	theme	CNF	1186:1188	arg1	films					1190:1194	the CNF films	1182:1194	the CNF films	1182:1194	The mechanical strength of the CNF films was further improved by exposing the hydroxyl group when lignin-carbohydrate complexes were de-structured by the enzymes.
29253942	7	8	theme	key	960:962	arg1	roles					964:968	key roles	960:968	key roles	960:968	It was found that the carboxyl and hydroxyl groups played key roles in affecting the dispersion and film properties of the CNF.
29253942	2	9	from	mechanism	290:298	arg1	properties					350:359	the dispersion and film properties	326:359	the dispersion and film properties of CNF	326:366	Moreover, the mechanism of enzymatic treatment on the dispersion and film properties of CNF was speculated.
29253942	3	10	theme	hemicellulose	526:538	arg1	composition					540:550	the hemicellulose composition	522:550	the hemicellulose composition	522:550	The xylanase pretreatment facilitated the deconstruction of pulp into CNF via mechanical shearing and high-pressure homogenization, while the hemicellulose composition was preserved.
29253942	0	11	theme	nanofibrils	90:100	arg1	properties					66:75	dispersion and film properties	46:75	dispersion and film properties of cellulose nanofibrils	46:100	Enzymatic pretreatment for the improvement of dispersion and film properties of cellulose nanofibrils.
29253942	6	12	theme	hemicellulose	887:899	arg1	removal					876:882	the removal	872:882	the removal of hemicellulose	872:899	The optical and mechanical strength properties of the CNF films were improved by the removal of hemicellulose.
29253942	7	13	theme	hydroxyl	937:944	arg1	groups					946:951	the carboxyl and hydroxyl groups	920:951	groups	946:951	It was found that the carboxyl and hydroxyl groups played key roles in affecting the dispersion and film properties of the CNF.
29253942	11	14	theme	CNF	1439:1441	arg1	suspension					1443:1452	the CNF suspension	1435:1452	the CNF suspension	1435:1452	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	4	15	theme	rheological	709:719	arg1	properties					721:730	rheological properties	709:730	rheological properties	709:730	Compared to the control, the CNF suspensions made from the xylanase pretreated pulp were more crystalline with higher dispersion, optical and rheological properties.
29253942	1	16	theme	present	110:116	arg1	study					118:122	the present study	106:122	the present study	106:122	In the present study, cellulose nanofibrils (CNF) were produced from unbleached eucalyptus pulp, and the effect of enzymatic treatment on the properties of CNF was studied.
29253942	8	17	theme	carboxyl	1034:1041	arg1	content					1049:1055	The carboxyl group content	1030:1055	The carboxyl group content	1030:1055	The carboxyl group content increased after the enzymatic pretreatment, which benefits the even distribution of CNF in water.
29253942	6	18	theme	optical	795:801	arg1	properties					827:836	The optical and mechanical strength properties	791:836	The optical and mechanical strength properties of the CNF films	791:853	The optical and mechanical strength properties of the CNF films were improved by the removal of hemicellulose.
29253942	2	19	theme	CNF	364:366	arg1	properties					350:359	the dispersion and film properties	326:359	the dispersion and film properties of CNF	326:366	Moreover, the mechanism of enzymatic treatment on the dispersion and film properties of CNF was speculated.
29253942	11	20	theme	theoretical	1396:1406	arg1	foundation					1408:1417	The theoretical foundation	1392:1417	The theoretical foundation for stabilizing the CNF suspension	1392:1452	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	7	21	theme	carboxyl	924:931	arg1	groups					946:951	the carboxyl and hydroxyl groups	920:951	groups	946:951	It was found that the carboxyl and hydroxyl groups played key roles in affecting the dispersion and film properties of the CNF.
29253942	11	22	dep	production	1544:1553	arg1	to					1536:1537	to	1536:1537	to	1536:1537	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	3	23	theme	mechanical	462:471	arg1	shearing					473:480	mechanical shearing	462:480	mechanical shearing	462:480	The xylanase pretreatment facilitated the deconstruction of pulp into CNF via mechanical shearing and high-pressure homogenization, while the hemicellulose composition was preserved.
29253942	8	24	theme	even	1120:1123	arg1	distribution					1125:1136	the even distribution	1116:1136	the even distribution of CNF in water	1116:1152	The carboxyl group content increased after the enzymatic pretreatment, which benefits the even distribution of CNF in water.
29253942	3	25	theme	xylanase	388:395	arg1	pretreatment					397:408	The xylanase pretreatment	384:408	The xylanase pretreatment	384:408	The xylanase pretreatment facilitated the deconstruction of pulp into CNF via mechanical shearing and high-pressure homogenization, while the hemicellulose composition was preserved.
29253942	0	26	theme	Enzymatic	0:8	arg1	pretreatment					10:21	Enzymatic pretreatment	0:21	Enzymatic pretreatment for the improvement of dispersion and film properties of cellulose nanofibrils.	0:101	Enzymatic pretreatment for the improvement of dispersion and film properties of cellulose nanofibrils.
29253942	8	27	theme	group	1043:1047	arg1	content					1049:1055	The carboxyl group content	1030:1055	The carboxyl group content	1030:1055	The carboxyl group content increased after the enzymatic pretreatment, which benefits the even distribution of CNF in water.
29253942	9	28	theme	films	1190:1194	arg1	strength					1170:1177	The mechanical strength	1155:1177	The mechanical strength of the CNF films	1155:1194	The mechanical strength of the CNF films was further improved by exposing the hydroxyl group when lignin-carbohydrate complexes were de-structured by the enzymes.
29253942	11	29	theme	high-speed	1462:1471	arg1	dehydration					1473:1483	the high-speed dehydration	1458:1483	the high-speed dehydration of CNF films	1458:1496	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	1	30	theme	unbleached	172:181	arg1	pulp					194:197	unbleached eucalyptus pulp	172:197	unbleached eucalyptus pulp	172:197	In the present study, cellulose nanofibrils (CNF) were produced from unbleached eucalyptus pulp, and the effect of enzymatic treatment on the properties of CNF was studied.
29253942	1	31	theme	CNF	259:261	arg1	properties					245:254	the properties	241:254	the properties of CNF	241:261	In the present study, cellulose nanofibrils (CNF) were produced from unbleached eucalyptus pulp, and the effect of enzymatic treatment on the properties of CNF was studied.
29253942	11	32	theme	CNF	1558:1560	arg1	film					1562:1565	CNF film	1558:1565	CNF film	1558:1565	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	9	33	theme	mechanical	1159:1168	arg1	strength					1170:1177	The mechanical strength	1155:1177	The mechanical strength of the CNF films	1155:1194	The mechanical strength of the CNF films was further improved by exposing the hydroxyl group when lignin-carbohydrate complexes were de-structured by the enzymes.
29253942	11	34	theme	roll	1539:1542	arg1	production					1544:1553	the continuous roll to roll production	1516:1553	the continuous roll to roll production of CNF film	1516:1565	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	1	35	theme	eucalyptus	183:192	arg1	pulp					194:197	unbleached eucalyptus pulp	172:197	unbleached eucalyptus pulp	172:197	In the present study, cellulose nanofibrils (CNF) were produced from unbleached eucalyptus pulp, and the effect of enzymatic treatment on the properties of CNF was studied.
29253942	10	36	theme	CNF	1357:1359	arg1	application					1342:1352	the application	1338:1352	the application of CNF produced from unbleached pulp	1338:1389	This study expanded the application of CNF produced from unbleached pulp.
29253942	4	37	with	crystalline	661:671	arg1	dispersion					685:694	higher dispersion	678:694	higher dispersion	678:694	Compared to the control, the CNF suspensions made from the xylanase pretreated pulp were more crystalline with higher dispersion, optical and rheological properties.
29253942	4	37	with	crystalline	661:671	arg1	optical					697:703	optical	697:703	optical	697:703	Compared to the control, the CNF suspensions made from the xylanase pretreated pulp were more crystalline with higher dispersion, optical and rheological properties.
29253942	4	37	with	crystalline	661:671	arg1	properties					721:730	rheological properties	709:730	rheological properties	709:730	Compared to the control, the CNF suspensions made from the xylanase pretreated pulp were more crystalline with higher dispersion, optical and rheological properties.
29253942	5	38	theme	filtration	771:780	arg1	process					782:788	a vacuum filtration process	762:788	a vacuum filtration process	762:788	CNF films were produced with a vacuum filtration process.
29253942	8	39	theme	enzymatic	1077:1085	arg1	pretreatment					1087:1098	the enzymatic pretreatment	1073:1098	the enzymatic pretreatment	1073:1098	The carboxyl group content increased after the enzymatic pretreatment, which benefits the even distribution of CNF in water.
29253942	8	40	from	distribution	1125:1136	arg1	water					1148:1152	water	1148:1152	water	1148:1152	The carboxyl group content increased after the enzymatic pretreatment, which benefits the even distribution of CNF in water.
29253942	2	41	theme	film	345:348	arg1	properties					350:359	the dispersion and film properties	326:359	the dispersion and film properties of CNF	326:366	Moreover, the mechanism of enzymatic treatment on the dispersion and film properties of CNF was speculated.
29253942	4	42	theme	pretreated	635:644	arg1	pulp					646:649	the xylanase pretreated pulp	622:649	the xylanase pretreated pulp	622:649	Compared to the control, the CNF suspensions made from the xylanase pretreated pulp were more crystalline with higher dispersion, optical and rheological properties.
29253942	5	43	theme	CNF	733:735	arg1	films					737:741	CNF films	733:741	CNF films	733:741	CNF films were produced with a vacuum filtration process.
29253942	7	44	theme	film	1002:1005	arg1	properties					1007:1016	the dispersion and film properties	983:1016	the dispersion and film properties of the CNF	983:1027	It was found that the carboxyl and hydroxyl groups played key roles in affecting the dispersion and film properties of the CNF.
29253942	4	45	theme	xylanase	626:633	arg1	pulp					646:649	the xylanase pretreated pulp	622:649	the xylanase pretreated pulp	622:649	Compared to the control, the CNF suspensions made from the xylanase pretreated pulp were more crystalline with higher dispersion, optical and rheological properties.
29253942	7	46	theme	CNF	1025:1027	arg1	properties					1007:1016	the dispersion and film properties	983:1016	the dispersion and film properties of the CNF	983:1027	It was found that the carboxyl and hydroxyl groups played key roles in affecting the dispersion and film properties of the CNF.
29253942	6	47	theme	films	849:853	arg1	properties					827:836	The optical and mechanical strength properties	791:836	The optical and mechanical strength properties of the CNF films	791:853	The optical and mechanical strength properties of the CNF films were improved by the removal of hemicellulose.
29253942	2	48	theme	treatment	313:321	arg1	mechanism					290:298	the mechanism	286:298	the mechanism of enzymatic treatment on the dispersion and film properties of CNF	286:366	Moreover, the mechanism of enzymatic treatment on the dispersion and film properties of CNF was speculated.
29253942	0	49	theme	dispersion	46:55	arg1	properties					66:75	dispersion and film properties	46:75	dispersion and film properties of cellulose nanofibrils	46:100	Enzymatic pretreatment for the improvement of dispersion and film properties of cellulose nanofibrils.
29253942	6	50	theme	CNF	845:847	arg1	films					849:853	the CNF films	841:853	the CNF films	841:853	The optical and mechanical strength properties of the CNF films were improved by the removal of hemicellulose.
29253942	7	51	theme	dispersion	987:996	arg1	properties					1007:1016	the dispersion and film properties	983:1016	the dispersion and film properties of the CNF	983:1027	It was found that the carboxyl and hydroxyl groups played key roles in affecting the dispersion and film properties of the CNF.
29253942	2	52	theme	enzymatic	303:311	arg1	treatment					313:321	enzymatic treatment	303:321	enzymatic treatment	303:321	Moreover, the mechanism of enzymatic treatment on the dispersion and film properties of CNF was speculated.
29253942	11	53	theme	films	1492:1496	arg1	foundation					1408:1417	The theoretical foundation	1392:1417	The theoretical foundation for stabilizing the CNF suspension	1392:1452	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	11	53	theme	films	1492:1496	arg1	dehydration					1473:1483	the high-speed dehydration	1458:1483	the high-speed dehydration of CNF films	1458:1496	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	11	54	theme	film	1562:1565	arg1	production					1544:1553	the continuous roll to roll production	1516:1553	the continuous roll to roll production of CNF film	1516:1565	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	9	55	theme	hydroxyl	1233:1240	arg1	group					1242:1246	the hydroxyl group	1229:1246	the hydroxyl group when lignin-carbohydrate complexes were de-structured by the enzymes	1229:1315	The mechanical strength of the CNF films was further improved by exposing the hydroxyl group when lignin-carbohydrate complexes were de-structured by the enzymes.
29253942	11	56	theme	roll	1531:1534	arg1	production					1544:1553	the continuous roll to roll production	1516:1553	the continuous roll to roll production of CNF film	1516:1565	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	6	57	theme	mechanical	807:816	arg1	properties					827:836	The optical and mechanical strength properties	791:836	The optical and mechanical strength properties of the CNF films	791:853	The optical and mechanical strength properties of the CNF films were improved by the removal of hemicellulose.
29253942	10	58	theme	unbleached	1375:1384	arg1	pulp					1386:1389	unbleached pulp	1375:1389	unbleached pulp	1375:1389	This study expanded the application of CNF produced from unbleached pulp.
29253942	4	59	theme	CNF	596:598	arg1	suspensions					600:610	the CNF suspensions	592:610	the CNF suspensions made from the xylanase pretreated pulp	592:649	Compared to the control, the CNF suspensions made from the xylanase pretreated pulp were more crystalline with higher dispersion, optical and rheological properties.
29253942	4	59	theme	CNF	596:598	arg1	crystalline					661:671	crystalline	661:671	crystalline	661:671	Compared to the control, the CNF suspensions made from the xylanase pretreated pulp were more crystalline with higher dispersion, optical and rheological properties.
29253942	1	60	from	effect	208:213	arg1	properties					245:254	the properties	241:254	the properties of CNF	241:261	In the present study, cellulose nanofibrils (CNF) were produced from unbleached eucalyptus pulp, and the effect of enzymatic treatment on the properties of CNF was studied.
29253942	1	61	theme	cellulose	125:133	arg1	CNF					148:150	CNF	148:150	CNF	148:150	In the present study, cellulose nanofibrils (CNF) were produced from unbleached eucalyptus pulp, and the effect of enzymatic treatment on the properties of CNF was studied.
29253942	1	61	theme	cellulose	125:133	arg1	nanofibrils					135:145	cellulose nanofibrils	125:145	cellulose nanofibrils (CNF)	125:151	In the present study, cellulose nanofibrils (CNF) were produced from unbleached eucalyptus pulp, and the effect of enzymatic treatment on the properties of CNF was studied.
29253942	0	62	theme	properties	66:75	arg1	improvement					31:41	the improvement	27:41	the improvement of dispersion and film properties of cellulose nanofibrils	27:100	Enzymatic pretreatment for the improvement of dispersion and film properties of cellulose nanofibrils.
29253942	3	63	theme	pulp	444:447	arg1	deconstruction					426:439	the deconstruction	422:439	the deconstruction of pulp into CNF	422:456	The xylanase pretreatment facilitated the deconstruction of pulp into CNF via mechanical shearing and high-pressure homogenization, while the hemicellulose composition was preserved.
29253942	4	64	theme	higher	678:683	arg1	dispersion					685:694	higher dispersion	678:694	higher dispersion	678:694	Compared to the control, the CNF suspensions made from the xylanase pretreated pulp were more crystalline with higher dispersion, optical and rheological properties.
29253942	11	65	theme	CNF	1488:1490	arg1	films					1492:1496	CNF films	1488:1496	CNF films	1488:1496	The theoretical foundation for stabilizing the CNF suspension and the high-speed dehydration of CNF films were explored for the continuous roll to roll production of CNF film.
29253942	0	66	theme	film	61:64	arg1	properties					66:75	dispersion and film properties	46:75	dispersion and film properties of cellulose nanofibrils	46:100	Enzymatic pretreatment for the improvement of dispersion and film properties of cellulose nanofibrils.
29253942	9	67	theme	lignin-carbohydrate	1253:1271	arg1	complexes					1273:1281	lignin-carbohydrate complexes	1253:1281	lignin-carbohydrate complexes	1253:1281	The mechanical strength of the CNF films was further improved by exposing the hydroxyl group when lignin-carbohydrate complexes were de-structured by the enzymes.
29253942	2	68	theme	dispersion	330:339	arg1	properties					350:359	the dispersion and film properties	326:359	the dispersion and film properties of CNF	326:366	Moreover, the mechanism of enzymatic treatment on the dispersion and film properties of CNF was speculated.
29253942	1	69	theme	enzymatic	218:226	arg1	treatment					228:236	enzymatic treatment	218:236	enzymatic treatment	218:236	In the present study, cellulose nanofibrils (CNF) were produced from unbleached eucalyptus pulp, and the effect of enzymatic treatment on the properties of CNF was studied.
28987883	10	0	theme	siRNA	1787:1791	arg1	delivery					1775:1782	targeted delivery	1766:1782	targeted delivery of siRNA	1766:1791	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	9	1	theme	~3	1513:1514	arg1	factor					1503:1508	a factor	1501:1508	a factor of ~3 with lesser renal clearance compared to non-targeted NPs	1501:1571	After intravenous (i.v.) injection to BALB/c mice, GalNAc-NPs were targeted to liver by a factor of ~3 with lesser renal clearance compared to non-targeted NPs.
28987883	3	2	theme	siRNA	466:470	arg1	nanoparticles					472:484	novel liver-targeted siRNA nanoparticles	445:484	novel liver-targeted siRNA nanoparticles (NPs)	445:490	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	2	theme	siRNA	466:470	arg1	bridges					594:600	co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges	493:600	co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs)	493:622	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	2	theme	siRNA	466:470	arg1	NPs					487:489	NPs	487:489	NPs	487:489	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	5	3	theme	similar	991:997	arg1	composition					1006:1016	a similar atomic composition	989:1016	a similar atomic composition	989:1016	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	8	4	theme	cell	1369:1372	arg1	uptake					1374:1379	cell uptake	1369:1379	cell uptake	1369:1379	Bioconjugation of N-acetylgalactosamine (GalNAc), a ligand to the asialoglycoprotein receptor (ASGPR) overexpressed on hepatocytes, was validated by XPS analysis and cell uptake by receptor-mediated mechanism.
28987883	3	5	theme	nanoparticles	472:484	arg1	features					433:440	the features	429:440	the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs)	429:622	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	5	6	theme	<-10mV	971:976	arg1	charge					961:966	surface charge	953:966	surface charge of <-10mV	953:976	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	8	7	theme	receptor-mediated	1384:1400	arg1	mechanism					1402:1410	receptor-mediated mechanism	1384:1410	receptor-mediated mechanism	1384:1410	Bioconjugation of N-acetylgalactosamine (GalNAc), a ligand to the asialoglycoprotein receptor (ASGPR) overexpressed on hepatocytes, was validated by XPS analysis and cell uptake by receptor-mediated mechanism.
28987883	9	8	theme	lesser	1521:1526	arg1	clearance					1534:1542	lesser renal clearance	1521:1542	lesser renal clearance	1521:1542	After intravenous (i.v.) injection to BALB/c mice, GalNAc-NPs were targeted to liver by a factor of ~3 with lesser renal clearance compared to non-targeted NPs.
28987883	5	9	theme	130-150nm	938:946	arg1	size					930:933	average size	922:933	average size of 130-150nm	922:946	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	3	10	theme	co-assembled	493:504	arg1	nanoparticles					472:484	novel liver-targeted siRNA nanoparticles	445:484	novel liver-targeted siRNA nanoparticles (NPs)	445:490	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	10	theme	co-assembled	493:504	arg1	bridges					594:600	co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges	493:600	co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs)	493:622	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	10	theme	co-assembled	493:504	arg1	NPs					619:621	AlgS-Ca2+-siRNA NPs	603:621	AlgS-Ca2+-siRNA NPs	603:621	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	10	11	theme	reversibility	1645:1657	arg1	advantages					1603:1612	the combined advantages	1590:1612	the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication	1590:1729	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	10	12	theme	tri-component	1666:1678	arg1	NPs					1680:1682	the tri-component NPs	1662:1682	the tri-component NPs	1662:1682	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	3	13	theme	targeting	653:661	arg1	ligand					663:668	a targeting ligand	651:668	a targeting ligand onto the AlgS upon the NP surface	651:702	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	5	14	theme	component	888:896	arg1	order					907:911	the component addition order	884:911	the component addition order	884:911	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	2	15	theme	delivery	279:286	arg1	vehicles					288:295	delivery vehicles	279:295	delivery vehicles	279:295	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	3	16	theme	ligand	663:668	arg1	bioconjugation					633:646	bioconjugation	633:646	bioconjugation of a targeting ligand onto the AlgS upon the NP surface	633:702	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	4	17	theme	AlgS	763:766	arg1	accessibility					768:780	AlgS accessibility	763:780	AlgS accessibility on NP surface	763:794	To gain insight into the complexation process and confirm AlgS accessibility on NP surface, we investigated different schemes for fabrication.
28987883	7	18	from	concentrations	1140:1153	arg1	NPs					1158:1160	NPs	1158:1160	NPs	1158:1160	Ca2+ and AlgS concentrations in NPs affected cell uptake and gene silencing.
28987883	5	19	theme	addition	898:905	arg1	order					907:911	the component addition order	884:911	the component addition order	884:911	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	2	20	theme	action	372:377	arg1	action					372:377	action	372:377	action	372:377	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	2	20	theme	action	372:377	arg1	site					364:367	the site	360:367	the site of action in the cells of target tissues	360:408	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	5	21	contain	had	949:951	arg1	NPs					862:864	All resulting NPs	848:864	All resulting NPs	848:864	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	5	21	contain	had	949:951	arg2	charge					961:966	surface charge	953:966	surface charge of <-10mV	953:976	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	2	22	theme	RNAi	273:276	arg1	potential					260:268	the clinical potential	247:268	the clinical potential of RNAi	247:276	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	5	23	theme	atomic	999:1004	arg1	composition					1006:1016	a similar atomic composition	989:1016	a similar atomic composition	989:1016	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	9	24	theme	intravenous	1419:1429	arg1	injection					1438:1446	intravenous (i.v.) injection	1419:1446	intravenous (i.v.) injection to BALB/c mice	1419:1461	After intravenous (i.v.) injection to BALB/c mice, GalNAc-NPs were targeted to liver by a factor of ~3 with lesser renal clearance compared to non-targeted NPs.
28987883	1	25	contain	has	149:151	arg1	RNAi					143:146	RNAi	143:146	RNAi	143:146	RNA interference (RNAi) has the potential to reversibly silence any gene with high efficiency and specificity.
28987883	1	25	contain	has	149:151	arg1	interference					129:140	RNA interference	125:140	RNA interference (RNAi)	125:147	RNA interference (RNAi) has the potential to reversibly silence any gene with high efficiency and specificity.
28987883	1	25	contain	has	149:151	arg2	potential					157:165	the potential to reversibly silence any gene with high efficiency and specificity	153:233	the potential to reversibly silence any gene with high efficiency and specificity	153:233	RNA interference (RNAi) has the potential to reversibly silence any gene with high efficiency and specificity.
28987883	8	26	theme	asialoglycoprotein	1269:1286	arg1	ASGPR					1298:1302	ASGPR	1298:1302	ASGPR	1298:1302	Bioconjugation of N-acetylgalactosamine (GalNAc), a ligand to the asialoglycoprotein receptor (ASGPR) overexpressed on hepatocytes, was validated by XPS analysis and cell uptake by receptor-mediated mechanism.
28987883	8	26	theme	asialoglycoprotein	1269:1286	arg1	receptor					1288:1295	the asialoglycoprotein receptor	1265:1295	the asialoglycoprotein receptor (ASGPR) overexpressed on hepatocytes	1265:1332	Bioconjugation of N-acetylgalactosamine (GalNAc), a ligand to the asialoglycoprotein receptor (ASGPR) overexpressed on hepatocytes, was validated by XPS analysis and cell uptake by receptor-mediated mechanism.
28987883	9	27	theme	BALB/c	1451:1456	arg1	mice					1458:1461	BALB/c mice	1451:1461	BALB/c mice	1451:1461	After intravenous (i.v.) injection to BALB/c mice, GalNAc-NPs were targeted to liver by a factor of ~3 with lesser renal clearance compared to non-targeted NPs.
28987883	4	28	from	accessibility	768:780	arg1	surface					788:794	NP surface	785:794	NP surface	785:794	To gain insight into the complexation process and confirm AlgS accessibility on NP surface, we investigated different schemes for fabrication.
28987883	0	29	theme	GalNAc	0:5	arg1	bio-functionalization					7:27	GalNAc bio-functionalization	0:27	GalNAc bio-functionalization of nanoparticles assembled by electrostatic interactions	0:84	GalNAc bio-functionalization of nanoparticles assembled by electrostatic interactions improves siRNA targeting to the liver.
28987883	9	30	theme	non-targeted	1556:1567	arg1	NPs					1569:1571	non-targeted NPs	1556:1571	non-targeted NPs	1556:1571	After intravenous (i.v.) injection to BALB/c mice, GalNAc-NPs were targeted to liver by a factor of ~3 with lesser renal clearance compared to non-targeted NPs.
28987883	10	31	theme	targeted	1766:1773	arg1	delivery					1775:1782	targeted delivery	1766:1782	targeted delivery of siRNA	1766:1791	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	9	32	with	~3	1513:1514	arg1	clearance					1534:1542	lesser renal clearance	1521:1542	lesser renal clearance	1521:1542	After intravenous (i.v.) injection to BALB/c mice, GalNAc-NPs were targeted to liver by a factor of ~3 with lesser renal clearance compared to non-targeted NPs.
28987883	0	33	theme	nanoparticles	32:44	arg1	bio-functionalization					7:27	GalNAc bio-functionalization	0:27	GalNAc bio-functionalization of nanoparticles assembled by electrostatic interactions	0:84	GalNAc bio-functionalization of nanoparticles assembled by electrostatic interactions improves siRNA targeting to the liver.
28987883	6	34	theme	~90	1096:1098	arg1	silencing					1101:1109	~90% silencing	1096:1109	~90% silencing of STAT3 gene	1096:1123	~90% silencing of STAT3 gene.
28987883	10	35	theme	NPs	1680:1682	arg1	targeting					1631:1639	site-specific targeting	1617:1639	site-specific targeting	1617:1639	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	10	35	theme	NPs	1680:1682	arg1	simplicity					1699:1708	the simplicity	1695:1708	site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication	1617:1729	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	10	35	theme	NPs	1680:1682	arg1	reversibility					1645:1657	reversibility	1645:1657	reversibility	1645:1657	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	7	36	theme	gene	1187:1190	arg1	silencing					1192:1200	gene silencing	1187:1200	gene silencing	1187:1200	Ca2+ and AlgS concentrations in NPs affected cell uptake and gene silencing.
28987883	2	37	theme	target	395:400	arg1	tissues					402:408	target tissues	395:408	target tissues	395:408	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	10	38	theme	site-specific	1617:1629	arg1	targeting					1631:1639	site-specific targeting	1617:1639	site-specific targeting	1617:1639	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	3	39	theme	calcium	581:587	arg1	ions					589:592	calcium ions	581:592	calcium ions	581:592	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	1	40	theme	RNA	125:127	arg1	RNAi					143:146	RNAi	143:146	RNAi	143:146	RNA interference (RNAi) has the potential to reversibly silence any gene with high efficiency and specificity.
28987883	1	40	theme	RNA	125:127	arg1	interference					129:140	RNA interference	125:140	RNA interference (RNAi)	125:147	RNA interference (RNAi) has the potential to reversibly silence any gene with high efficiency and specificity.
28987883	10	41	theme	targeting	1631:1639	arg1	advantages					1603:1612	the combined advantages	1590:1612	the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication	1590:1729	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	1	42	theme	high	203:206	arg1	efficiency					208:217	high efficiency	203:217	high efficiency	203:217	RNA interference (RNAi) has the potential to reversibly silence any gene with high efficiency and specificity.
28987883	3	43	theme	liver-targeted	451:464	arg1	nanoparticles					472:484	novel liver-targeted siRNA nanoparticles	445:484	novel liver-targeted siRNA nanoparticles (NPs)	445:490	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	43	theme	liver-targeted	451:464	arg1	bridges					594:600	co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges	493:600	co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs)	493:622	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	43	theme	liver-targeted	451:464	arg1	NPs					487:489	NPs	487:489	NPs	487:489	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	6	44	theme	STAT3	1114:1118	arg1	gene					1120:1123	STAT3 gene	1114:1123	STAT3 gene	1114:1123	~90% silencing of STAT3 gene.
28987883	4	45	theme	complexation	730:741	arg1	process					743:749	the complexation process	726:749	the complexation process	726:749	To gain insight into the complexation process and confirm AlgS accessibility on NP surface, we investigated different schemes for fabrication.
28987883	2	46	theme	tissues	402:408	arg1	cells					386:390	the cells	382:390	the cells of target tissues	382:408	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	2	47	from	site	364:367	arg1	cells					386:390	the cells	382:390	the cells of target tissues	382:408	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	5	48	theme	HepG2	1064:1068	arg1	cells					1070:1074	HepG2 cells	1064:1074	HepG2 cells	1064:1074	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	10	49	theme	combined	1594:1601	arg1	advantages					1603:1612	the combined advantages	1590:1612	the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication	1590:1729	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	4	50	theme	different	813:821	arg1	schemes					823:829	different schemes	813:829	different schemes for fabrication	813:845	To gain insight into the complexation process and confirm AlgS accessibility on NP surface, we investigated different schemes for fabrication.
28987883	10	51	theme	fabrication	1719:1729	arg1	targeting					1631:1639	site-specific targeting	1617:1639	site-specific targeting	1617:1639	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	10	51	theme	fabrication	1719:1729	arg1	simplicity					1699:1708	the simplicity	1695:1708	site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication	1617:1729	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	10	51	theme	fabrication	1719:1729	arg1	reversibility					1645:1657	reversibility	1645:1657	reversibility	1645:1657	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	3	52	theme	alginate	533:540	arg1	AlgS					551:554	AlgS	551:554	AlgS	551:554	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	52	theme	alginate	533:540	arg1	sulfate					542:548	alginate sulfate	533:548	alginate sulfate (AlgS)	533:555	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	53	theme	novel	445:449	arg1	nanoparticles					472:484	novel liver-targeted siRNA nanoparticles	445:484	novel liver-targeted siRNA nanoparticles (NPs)	445:490	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	53	theme	novel	445:449	arg1	bridges					594:600	co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges	493:600	co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs)	493:622	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	53	theme	novel	445:449	arg1	NPs					487:489	NPs	487:489	NPs	487:489	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	0	54	theme	electrostatic	59:71	arg1	interactions					73:84	electrostatic interactions	59:84	electrostatic interactions	59:84	GalNAc bio-functionalization of nanoparticles assembled by electrostatic interactions improves siRNA targeting to the liver.
28987883	3	55	theme	AlgS-Ca2+-siRNA	603:617	arg1	bridges					594:600	co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges	493:600	co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs)	493:622	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	55	theme	AlgS-Ca2+-siRNA	603:617	arg1	NPs					619:621	AlgS-Ca2+-siRNA NPs	603:621	AlgS-Ca2+-siRNA NPs	603:621	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	3	56	theme	sulfate	542:548	arg1	complexation					517:528	the complexation	513:528	the complexation	513:528	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	9	57	theme	renal	1528:1532	arg1	clearance					1534:1542	lesser renal clearance	1521:1542	lesser renal clearance	1521:1542	After intravenous (i.v.) injection to BALB/c mice, GalNAc-NPs were targeted to liver by a factor of ~3 with lesser renal clearance compared to non-targeted NPs.
28987883	2	58	theme	interfering	333:343	arg1	siRNA					350:354	siRNA	350:354	siRNA	350:354	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	2	58	theme	interfering	333:343	arg1	RNA					345:347	the short interfering RNA	323:347	the short interfering RNA (siRNA)	323:355	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	3	59	theme	NP	693:694	arg1	surface					696:702	the NP surface	689:702	the NP surface	689:702	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	8	60	theme	XPS	1352:1354	arg1	analysis					1356:1363	XPS analysis	1352:1363	XPS analysis	1352:1363	Bioconjugation of N-acetylgalactosamine (GalNAc), a ligand to the asialoglycoprotein receptor (ASGPR) overexpressed on hepatocytes, was validated by XPS analysis and cell uptake by receptor-mediated mechanism.
28987883	5	61	theme	surface	953:959	arg1	charge					961:966	surface charge	953:966	surface charge of <-10mV	953:976	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	6	62	theme	gene	1120:1123	arg1	silencing					1101:1109	~90% silencing	1096:1109	~90% silencing of STAT3 gene	1096:1123	~90% silencing of STAT3 gene.
28987883	2	63	theme	short	327:331	arg1	siRNA					350:354	siRNA	350:354	siRNA	350:354	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	2	63	theme	short	327:331	arg1	RNA					345:347	the short interfering RNA	323:347	the short interfering RNA (siRNA)	323:355	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	9	64	theme	i.v.	1432:1435	arg1	injection					1438:1446	intravenous (i.v.) injection	1419:1446	intravenous (i.v.) injection to BALB/c mice	1419:1461	After intravenous (i.v.) injection to BALB/c mice, GalNAc-NPs were targeted to liver by a factor of ~3 with lesser renal clearance compared to non-targeted NPs.
28987883	8	65	gly	asialoglycoprotein	1269:1286	arg1	asialoglycoprotein					1269:1286	the asialoglycoprotein receptor	1265:1295	the asialoglycoprotein receptor (ASGPR) overexpressed on hepatocytes	1265:1332	Bioconjugation of N-acetylgalactosamine (GalNAc), a ligand to the asialoglycoprotein receptor (ASGPR) overexpressed on hepatocytes, was validated by XPS analysis and cell uptake by receptor-mediated mechanism.
28987883	7	66	theme	cell	1171:1174	arg1	uptake					1176:1181	cell uptake	1171:1181	cell uptake	1171:1181	Ca2+ and AlgS concentrations in NPs affected cell uptake and gene silencing.
28987883	6	67	theme	%	1099:1099	arg1	silencing					1101:1109	~90% silencing	1096:1109	~90% silencing of STAT3 gene	1096:1123	~90% silencing of STAT3 gene.
28987883	3	68	with	complexation	517:528	arg1	siRNA					562:566	siRNA	562:566	siRNA	562:566	Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca2+-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface.
28987883	5	69	theme	resulting	852:860	arg1	NPs					862:864	All resulting NPs	848:864	All resulting NPs	848:864	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	8	70	theme	N-acetylgalactosamine	1221:1241	arg1	Bioconjugation					1203:1216	Bioconjugation	1203:1216	Bioconjugation of N-acetylgalactosamine (GalNAc), a ligand to the asialoglycoprotein receptor (ASGPR) overexpressed on hepatocytes,	1203:1333	Bioconjugation of N-acetylgalactosamine (GalNAc), a ligand to the asialoglycoprotein receptor (ASGPR) overexpressed on hepatocytes, was validated by XPS analysis and cell uptake by receptor-mediated mechanism.
28987883	0	71	theme	siRNA	95:99	arg1	targeting					101:109	siRNA targeting	95:109	siRNA targeting	95:109	GalNAc bio-functionalization of nanoparticles assembled by electrostatic interactions improves siRNA targeting to the liver.
28987883	10	72	theme	attractive	1744:1753	arg1	system					1755:1760	an attractive system	1741:1760	an attractive system for targeted delivery of siRNA	1741:1791	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	2	73	theme	clinical	251:258	arg1	potential					260:268	the clinical potential	247:268	the clinical potential of RNAi	247:276	To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues.
28987883	7	74	theme	AlgS	1135:1138	arg1	concentrations					1140:1153	Ca2+ and AlgS concentrations	1126:1153	Ca2+ and AlgS concentrations in NPs	1126:1160	Ca2+ and AlgS concentrations in NPs affected cell uptake and gene silencing.
28987883	5	75	theme	average	922:928	arg1	size					930:933	average size	922:933	average size of 130-150nm	922:946	All resulting NPs, independently of the component addition order, were of average size of 130-150nm, had surface charge of <-10mV, exhibited a similar atomic composition on their surface, were efficiently uptaken by HepG2 cells and induced approx.
28987883	10	76	theme	simplicity	1699:1708	arg1	advantages					1603:1612	the combined advantages	1590:1612	the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication	1590:1729	We foresee that the combined advantages of site-specific targeting and reversibility of the tri-component NPs as well as the simplicity of their fabrication make them an attractive system for targeted delivery of siRNA.
28987883	7	77	theme	Ca2+	1126:1129	arg1	concentrations					1140:1153	Ca2+ and AlgS concentrations	1126:1153	Ca2+ and AlgS concentrations in NPs	1126:1160	Ca2+ and AlgS concentrations in NPs affected cell uptake and gene silencing.
24801679	5	0	theme	enteric	723:729	arg1	polymer					731:737	enteric polymer	723:737	enteric polymer	723:737	LBC of a model live bacterial vaccine and a probiotic were dried directly onto a cast film of enteric polymer.
24801679	2	1	theme	antimicrobial	340:352	arg1	defenses					354:361	GI antimicrobial defenses	337:361	GI antimicrobial defenses including gastric acid	337:384	However, LBCs present unique formulation challenges and must survive GI antimicrobial defenses including gastric acid after administration.
24801679	2	1	theme	antimicrobial	340:352	arg1	acid					381:384	gastric acid	373:384	gastric acid	373:384	However, LBCs present unique formulation challenges and must survive GI antimicrobial defenses including gastric acid after administration.
24801679	1	2	theme	gastrointestinal	238:253	arg1	health					260:265	gastrointestinal (GI) health	238:265	gastrointestinal (GI) health	238:265	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	0	3	theme	vaccine	66:72	arg1	delivery					49:56	enteric delivery	41:56	enteric delivery of live vaccine and probiotic bacteria	41:95	A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria.
24801679	7	4	theme	polymer	1038:1044	arg1	films					1046:1050	blended polymer films	1030:1050	blended polymer films	1030:1050	However, although PFL made from blended polymer films completely released low-molecular-weight dye into intestinal conditions (pH 7.0), they failed to release LBCs.
24801679	6	5	theme	simulated	789:797	arg1	fluid					807:811	a simulated gastric fluid	787:811	a simulated gastric fluid (SGF, pH 2.0)	787:825	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	2	6	theme	GI	337:338	arg1	defenses					354:361	GI antimicrobial defenses	337:361	GI antimicrobial defenses including gastric acid	337:384	However, LBCs present unique formulation challenges and must survive GI antimicrobial defenses including gastric acid after administration.
24801679	2	6	theme	GI	337:338	arg1	acid					381:384	gastric acid	373:384	gastric acid	373:384	However, LBCs present unique formulation challenges and must survive GI antimicrobial defenses including gastric acid after administration.
24801679	6	7	theme	Eudragit	921:928	arg1	L100					930:933	Eudragit L100 55	921:936	Eudragit L100 55 providing greater protection from acid than Eudragit alone	921:995	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	0	8	theme	bacteria	88:95	arg1	delivery					49:56	enteric delivery	41:56	enteric delivery of live vaccine and probiotic bacteria	41:95	A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria.
24801679	7	9	theme	blended	1030:1036	arg1	films					1046:1050	blended polymer films	1030:1050	blended polymer films	1030:1050	However, although PFL made from blended polymer films completely released low-molecular-weight dye into intestinal conditions (pH 7.0), they failed to release LBCs.
24801679	6	10	theme	dried	772:776	arg1	cells					778:782	dried cells	772:782	dried cells	772:782	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	0	11	theme	probiotic	78:86	arg1	bacteria					88:95	probiotic bacteria	78:95	probiotic bacteria	78:95	A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria.
24801679	5	12	theme	model	638:642	arg1	vaccine					659:665	a model live bacterial vaccine	636:665	a model live bacterial vaccine	636:665	LBC of a model live bacterial vaccine and a probiotic were dried directly onto a cast film of enteric polymer.
24801679	6	13	theme	film	874:877	arg1	composition					843:853	the composition	839:853	the composition of enteric polymer film used	839:882	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	9	14	theme	lamination	1433:1442	arg1	method					1444:1449	the lamination method	1429:1449	the lamination method	1429:1449	Release kinetics could be controlled by modifying the lamination method.
24801679	6	15	theme	ethylcellulose	901:914	arg1	blend					892:896	a blend	890:896	a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone	890:995	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	4	16	theme	oral	611:614	arg1	form					623:626	a solid oral dosage form	603:626	a solid oral dosage form	603:626	LBCs are ambient dried onto cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form.
24801679	3	17	theme	formulation	432:442	arg1	concept					444:450	a simple new formulation concept	419:450	a simple new formulation concept	419:450	We present a simple new formulation concept, termed polymer film laminate (PFL).
24801679	5	18	theme	cast	710:713	arg1	film					715:718	a cast film	708:718	a cast film of enteric polymer	708:737	LBC of a model live bacterial vaccine and a probiotic were dried directly onto a cast film of enteric polymer.
24801679	6	19	theme	L100	930:933	arg1	blend					892:896	a blend	890:896	a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone	890:995	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	6	20	from	acid	972:975	arg1	protection					956:965	greater protection	948:965	greater protection from acid than Eudragit alone	948:995	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	1	21	theme	attenuated	153:162	arg1	vaccines					164:171	attenuated vaccines	153:171	attenuated vaccines	153:171	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	1	21	theme	attenuated	153:162	arg1	cells					113:117	Live bacterial cells	98:117	Live bacterial cells (LBCs)	98:124	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	0	22	theme	laminated	2:10	arg1	formulation					25:35	A laminated polymer film formulation	0:35	A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria	0:95	A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria.
24801679	5	23	theme	polymer	731:737	arg1	film					715:718	a cast film	708:718	a cast film of enteric polymer	708:737	LBC of a model live bacterial vaccine and a probiotic were dried directly onto a cast film of enteric polymer.
24801679	2	24	theme	formulation	297:307	arg1	challenges					309:318	unique formulation challenges	290:318	unique formulation challenges	290:318	However, LBCs present unique formulation challenges and must survive GI antimicrobial defenses including gastric acid after administration.
24801679	3	25	theme	simple	421:426	arg1	concept					444:450	a simple new formulation concept	419:450	a simple new formulation concept	419:450	We present a simple new formulation concept, termed polymer film laminate (PFL).
24801679	4	26	theme	polymer	545:551	arg1	films					553:557	cast acid-resistant enteric polymer films	517:557	cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form	517:626	LBCs are ambient dried onto cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form.
24801679	2	27	theme	unique	290:295	arg1	challenges					309:318	unique formulation challenges	290:318	unique formulation challenges	290:318	However, LBCs present unique formulation challenges and must survive GI antimicrobial defenses including gastric acid after administration.
24801679	2	28	theme	gastric	373:379	arg1	acid					381:384	gastric acid	373:384	gastric acid	373:384	However, LBCs present unique formulation challenges and must survive GI antimicrobial defenses including gastric acid after administration.
24801679	0	29	theme	film	20:23	arg1	formulation					25:35	A laminated polymer film formulation	0:35	A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria	0:95	A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria.
24801679	4	30	theme	enteric	537:543	arg1	films					553:557	cast acid-resistant enteric polymer films	517:557	cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form	517:626	LBCs are ambient dried onto cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form.
24801679	8	31	theme	vaccine	1247:1253	arg1	LBC					1255:1257	vaccine LBC	1247:1257	vaccine LBC	1247:1257	In contrast, PFL made from Eudragit alone successfully protected dried probiotic or vaccine LBC from SGF for 2 h, and subsequently released all viable cells within 60 min of transfer into simulated intestinal fluid.
24801679	3	32	theme	polymer	460:466	arg1	PFL					483:485	PFL	483:485	PFL	483:485	We present a simple new formulation concept, termed polymer film laminate (PFL).
24801679	3	32	theme	polymer	460:466	arg1	film					468:471	polymer film laminate	460:480	polymer film laminate (PFL)	460:486	We present a simple new formulation concept, termed polymer film laminate (PFL).
24801679	0	33	theme	polymer	12:18	arg1	formulation					25:35	A laminated polymer film formulation	0:35	A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria	0:95	A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria.
24801679	6	34	dep	fluid	807:811	arg1	SGF					814:816	SGF	814:816	SGF	814:816	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	6	34	dep	fluid	807:811	arg1	pH					819:820	pH 2.0	819:824	pH 2.0	819:824	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	1	35	theme	Live	98:101	arg1	vaccines					164:171	attenuated vaccines	153:171	attenuated vaccines	153:171	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	1	35	theme	Live	98:101	arg1	LBCs					120:123	LBCs	120:123	LBCs	120:123	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	1	35	theme	Live	98:101	arg1	cells					113:117	Live bacterial cells	98:117	Live bacterial cells (LBCs)	98:124	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	1	36	theme	biopharmaceutical	184:200	arg1	agents					202:207	biopharmaceutical agents	184:207	biopharmaceutical agents	184:207	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	8	37	theme	transfer	1337:1344	arg1	min					1330:1332	60 min	1327:1332	60 min of transfer into simulated intestinal fluid	1327:1376	In contrast, PFL made from Eudragit alone successfully protected dried probiotic or vaccine LBC from SGF for 2 h, and subsequently released all viable cells within 60 min of transfer into simulated intestinal fluid.
24801679	3	38	theme	new	428:430	arg1	concept					444:450	a simple new formulation concept	419:450	a simple new formulation concept	419:450	We present a simple new formulation concept, termed polymer film laminate (PFL).
24801679	9	39	theme	Release	1379:1385	arg1	kinetics					1387:1394	Release kinetics	1379:1394	Release kinetics	1379:1394	Release kinetics could be controlled by modifying the lamination method.
24801679	8	40	theme	intestinal	1361:1370	arg1	fluid					1372:1376	simulated intestinal fluid	1351:1376	simulated intestinal fluid	1351:1376	In contrast, PFL made from Eudragit alone successfully protected dried probiotic or vaccine LBC from SGF for 2 h, and subsequently released all viable cells within 60 min of transfer into simulated intestinal fluid.
24801679	7	41	theme	intestinal	1102:1111	arg1	conditions					1113:1122	intestinal conditions	1102:1122	intestinal conditions (pH 7.0)	1102:1131	However, although PFL made from blended polymer films completely released low-molecular-weight dye into intestinal conditions (pH 7.0), they failed to release LBCs.
24801679	7	41	theme	intestinal	1102:1111	arg1	pH					1125:1126	pH 7.0	1125:1130	pH 7.0	1125:1130	However, although PFL made from blended polymer films completely released low-molecular-weight dye into intestinal conditions (pH 7.0), they failed to release LBCs.
24801679	6	42	theme	polymer	866:872	arg1	film					874:877	enteric polymer film	858:877	enteric polymer film used	858:882	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	5	43	theme	live	644:647	arg1	vaccine					659:665	a model live bacterial vaccine	636:665	a model live bacterial vaccine	636:665	LBC of a model live bacterial vaccine and a probiotic were dried directly onto a cast film of enteric polymer.
24801679	8	44	theme	simulated	1351:1359	arg1	fluid					1372:1376	simulated intestinal fluid	1351:1376	simulated intestinal fluid	1351:1376	In contrast, PFL made from Eudragit alone successfully protected dried probiotic or vaccine LBC from SGF for 2 h, and subsequently released all viable cells within 60 min of transfer into simulated intestinal fluid.
24801679	1	45	theme	bacterial	103:111	arg1	vaccines					164:171	attenuated vaccines	153:171	attenuated vaccines	153:171	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	1	45	theme	bacterial	103:111	arg1	LBCs					120:123	LBCs	120:123	LBCs	120:123	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	1	45	theme	bacterial	103:111	arg1	cells					113:117	Live bacterial cells	98:117	Live bacterial cells (LBCs)	98:124	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	0	46	theme	enteric	41:47	arg1	delivery					49:56	enteric delivery	41:56	enteric delivery of live vaccine and probiotic bacteria	41:95	A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria.
24801679	4	47	theme	acid-resistant	522:535	arg1	films					553:557	cast acid-resistant enteric polymer films	517:557	cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form	517:626	LBCs are ambient dried onto cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form.
24801679	5	48	theme	bacterial	649:657	arg1	vaccine					659:665	a model live bacterial vaccine	636:665	a model live bacterial vaccine	636:665	LBC of a model live bacterial vaccine and a probiotic were dried directly onto a cast film of enteric polymer.
24801679	8	49	theme	viable	1307:1312	arg1	cells					1314:1318	all viable cells	1303:1318	all viable cells	1303:1318	In contrast, PFL made from Eudragit alone successfully protected dried probiotic or vaccine LBC from SGF for 2 h, and subsequently released all viable cells within 60 min of transfer into simulated intestinal fluid.
24801679	4	50	theme	cast	517:520	arg1	films					553:557	cast acid-resistant enteric polymer films	517:557	cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form	517:626	LBCs are ambient dried onto cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form.
24801679	5	51	theme	vaccine	659:665	arg1	LBC					629:631	LBC	629:631	LBC of a model live bacterial vaccine	629:665	LBC of a model live bacterial vaccine and a probiotic were dried directly onto a cast film of enteric polymer.
24801679	6	52	theme	gastric	799:805	arg1	fluid					807:811	a simulated gastric fluid	787:811	a simulated gastric fluid (SGF, pH 2.0)	787:825	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	7	53	theme	low-molecular-weight	1072:1091	arg1	dye					1093:1095	low-molecular-weight dye	1072:1095	low-molecular-weight dye	1072:1095	However, although PFL made from blended polymer films completely released low-molecular-weight dye into intestinal conditions (pH 7.0), they failed to release LBCs.
24801679	1	54	dep	gastrointestinal	238:253	arg1	GI					256:257	GI	256:257	GI	256:257	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	6	55	theme	greater	948:954	arg1	protection					956:965	greater protection	948:965	greater protection from acid than Eudragit alone	948:995	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	0	56	theme	live	61:64	arg1	vaccine					66:72	live vaccine	61:72	live vaccine	61:72	A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria.
24801679	1	57	dep	deliver	176:182	arg1	improve					230:236	improve	230:236	to improve gastrointestinal (GI) health	227:265	Live bacterial cells (LBCs) are administered orally as attenuated vaccines to deliver biopharmaceutical agents and as probiotics to improve gastrointestinal (GI) health.
24801679	4	58	theme	dosage	616:621	arg1	form					623:626	a solid oral dosage form	603:626	a solid oral dosage form	603:626	LBCs are ambient dried onto cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form.
24801679	4	59	theme	solid	605:609	arg1	form					623:626	a solid oral dosage form	603:626	a solid oral dosage form	603:626	LBCs are ambient dried onto cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form.
24801679	6	60	theme	enteric	858:864	arg1	film					874:877	enteric polymer film	858:877	enteric polymer film used	858:882	The effectiveness at protecting dried cells in a simulated gastric fluid (SGF, pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone.
24801679	3	61	dep	film	468:471	arg1	laminate					473:480	laminate	473:480	laminate	473:480	We present a simple new formulation concept, termed polymer film laminate (PFL).
24718964	7	0	theme	amiloride	966:974	arg1	Addition					954:961	Addition	954:961	Addition of amiloride	954:974	Addition of amiloride led to significantly increased motility (P < 0.05) compared with sucrose (Na(+) free) solutions containing 2 mM EGTA.
24718964	4	1	theme	reaction	482:489	arg1	investigation					453:465	investigation	453:465	investigation of spermatozoa reaction to external Ca(2+) concentration	453:522	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	2	2	theme	mature	277:282	arg1	males					284:288	ten mature males	273:288	ten mature males	273:288	Milt from ten mature males was obtained by abdominal massage.
24718964	4	3	theme	external	494:501	arg1	2+					506:507	2+	506:507	2+	506:507	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	4	3	theme	external	494:501	arg1	Ca					503:504	external Ca	494:504	external Ca(2+) concentration	494:522	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	7	4	contain	containing	1072:1081	arg1	solutions					1062:1070	sucrose (Na(+) free) solutions	1041:1070	sucrose (Na(+) free) solutions containing 2 mM EGTA	1041:1091	Addition of amiloride led to significantly increased motility (P < 0.05) compared with sucrose (Na(+) free) solutions containing 2 mM EGTA.
24718964	7	4	contain	containing	1072:1081	arg2	EGTA					1088:1091	2 mM EGTA	1083:1091	2 mM EGTA	1083:1091	Addition of amiloride led to significantly increased motility (P < 0.05) compared with sucrose (Na(+) free) solutions containing 2 mM EGTA.
24718964	4	5	theme	glycol	539:544	arg1	chelator					622:629	a calcium ions chelator	607:629	a calcium ions chelator	607:629	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	4	5	theme	glycol	539:544	arg1	EGTA					564:567	EGTA	564:567	EGTA	564:567	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	4	5	theme	glycol	539:544	arg1	acid					558:561	2 mM ethylene glycol tetraacetic acid	525:561	2 mM ethylene glycol tetraacetic acid (EGTA)	525:568	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	3	6	theme	Tris-HCl	432:439	arg1	pH					441:442	10 mM Tris-HCl pH 8.5	426:446	10 mM Tris-HCl pH 8.5	426:446	Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5.
24718964	9	7	theme	spermatozoa	1289:1299	arg1	activation					1301:1310	spermatozoa activation	1289:1310	spermatozoa activation	1289:1310	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	1	8	theme	environmental	122:134	arg1	composition					140:150	environmental ion composition	122:150	environmental ion composition	122:150	The role of environmental ion composition and osmolality in Ca(2+) signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis.
24718964	7	9	theme	mM	1085:1086	arg1	EGTA					1088:1091	2 mM EGTA	1083:1091	2 mM EGTA	1083:1091	Addition of amiloride led to significantly increased motility (P < 0.05) compared with sucrose (Na(+) free) solutions containing 2 mM EGTA.
24718964	0	10	theme	trout	58:62	arg1	motility					100:107	brook trout (Salvelinus fontinalis) spermatozoa motility	52:107	brook trout (Salvelinus fontinalis) spermatozoa motility	52:107	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.
24718964	1	11	theme	composition	140:150	arg1	role					114:117	The role	110:117	The role of environmental ion composition and osmolality in Ca(2+) signaled activation	110:195	The role of environmental ion composition and osmolality in Ca(2+) signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis.
24718964	6	12	contain	containing	884:893	arg2	EGTA					900:903	2 mM EGTA	895:903	2 mM EGTA	895:903	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	6	12	contain	containing	884:893	arg1	solutions					874:882	sucrose (Na(+) free) solutions	853:882	sucrose (Na(+) free) solutions containing 2 mM EGTA	853:903	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	7	13	theme	sucrose	1041:1047	arg1	solutions					1062:1070	sucrose (Na(+) free) solutions	1041:1070	sucrose (Na(+) free) solutions containing 2 mM EGTA	1041:1091	Addition of amiloride led to significantly increased motility (P < 0.05) compared with sucrose (Na(+) free) solutions containing 2 mM EGTA.
24718964	4	14	theme	mM	527:528	arg1	glycol					539:544	2 mM ethylene glycol	525:544	2 mM ethylene glycol tetraacetic acid (EGTA)	525:568	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	0	15	theme	Salvelinus	65:74	arg1	trout					58:62	brook trout	52:62	brook trout (Salvelinus fontinalis) spermatozoa motility	52:107	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.
24718964	0	15	theme	Salvelinus	65:74	arg1	fontinalis					76:85	Salvelinus fontinalis	65:85	Salvelinus fontinalis	65:85	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.
24718964	5	16	theme	Na	676:677	arg1	concentration					682:694	external Na(+) concentration	667:694	external Na(+) concentration in conditions of low external Ca(2+)	667:731	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	5	17	from	concentration	682:694	arg1	conditions					699:708	conditions	699:708	conditions of low external Ca(2+)	699:731	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	1	18	theme	osmolality	156:165	arg1	role					114:117	The role	110:117	The role of environmental ion composition and osmolality in Ca(2+) signaled activation	110:195	The role of environmental ion composition and osmolality in Ca(2+) signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis.
24718964	5	19	theme	100	734:736	arg1	µM					738:739	µM	738:739	µM	738:739	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	6	20	theme	mM	945:946	arg1	EGTA					948:951	2 mM EGTA	943:951	2 mM EGTA	943:951	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	4	21	theme	activation	587:596	arg1	media					598:602	the activation media	583:602	the activation media	583:602	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	8	22	from	transport	1117:1125	arg1	solutions					1142:1150	Ca(2+)-free solutions	1130:1150	Ca(2+)-free solutions	1130:1150	We conclude that Na(+) transport in Ca(2+)-free solutions plays a regulatory role in brook trout spermatozoa activation.
24718964	5	23	from	conditions	699:708	arg1	effect					657:662	the effect	653:662	the effect of external Na(+) concentration in conditions of low external Ca(2+)	653:731	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	8	24	theme	-free	1136:1140	arg1	solutions					1142:1150	Ca(2+)-free solutions	1130:1150	Ca(2+)-free solutions	1130:1150	We conclude that Na(+) transport in Ca(2+)-free solutions plays a regulatory role in brook trout spermatozoa activation.
24718964	9	25	theme	activation	1397:1406	arg1	improvement					1371:1381	improvement	1371:1381	improvement of artificial activation and storage media	1371:1424	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	0	26	theme	membrane	30:37	arg1	transport					39:47	Na (+) membrane transport	23:47	Na (+) membrane transport	23:47	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.
24718964	9	27	theme	storage	1412:1418	arg1	media					1420:1424	storage media	1412:1424	storage media	1412:1424	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	8	28	theme	regulatory	1160:1169	arg1	role					1171:1174	a regulatory role	1158:1174	a regulatory role	1158:1174	We conclude that Na(+) transport in Ca(2+)-free solutions plays a regulatory role in brook trout spermatozoa activation.
24718964	2	29	theme	abdominal	306:314	arg1	massage					316:322	abdominal massage	306:322	abdominal massage	306:322	Milt from ten mature males was obtained by abdominal massage.
24718964	5	30	theme	external	667:674	arg1	Na					676:677	external Na	667:677	external Na	667:677	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	6	31	theme	sucrose	853:859	arg1	solutions					874:882	sucrose (Na(+) free) solutions	853:882	sucrose (Na(+) free) solutions containing 2 mM EGTA	853:903	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	8	32	theme	trout	1185:1189	arg1	activation					1203:1212	brook trout spermatozoa activation	1179:1212	brook trout spermatozoa activation	1179:1212	We conclude that Na(+) transport in Ca(2+)-free solutions plays a regulatory role in brook trout spermatozoa activation.
24718964	4	33	theme	calcium	609:615	arg1	ions					617:620	calcium ions	609:620	a calcium ions chelator	607:629	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	5	34	theme	transport	805:813	arg1	blocker					815:821	a Na(+) transport blocker	797:821	a Na(+) transport blocker	797:821	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	5	34	theme	transport	805:813	arg1	amiloride					741:749	100 µM amiloride	734:749	100 µM amiloride	734:749	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	9	35	theme	competitive	1232:1242	arg1	Na					1244:1245	competitive Na	1232:1245	competitive Na	1232:1245	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	1	36	theme	brook	228:232	arg1	fontinalis					251:260	brook trout Salvelinus fontinalis	228:260	brook trout Salvelinus fontinalis	228:260	The role of environmental ion composition and osmolality in Ca(2+) signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis.
24718964	7	37	dep	sucrose	1041:1047	arg1	free					1056:1059	free	1056:1059	free	1056:1059	Addition of amiloride led to significantly increased motility (P < 0.05) compared with sucrose (Na(+) free) solutions containing 2 mM EGTA.
24718964	3	38	theme	Spermatozoa	325:335	arg1	motility					337:344	Spermatozoa motility	325:344	Spermatozoa motility	325:344	Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5.
24718964	6	39	contain	containing	932:941	arg1	solutions					922:930	Na(+) solutions	916:930	Na(+) solutions containing 2 mM EGTA	916:951	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	6	39	contain	containing	932:941	arg2	EGTA					948:951	2 mM EGTA	943:951	2 mM EGTA	943:951	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	0	40	theme	Na	23:24	arg1	transport					39:47	Na (+) membrane transport	23:47	Na (+) membrane transport	23:47	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.
24718964	3	41	theme	sucrose	395:401	arg1	solutions					403:411	0, 100, and 300 mOsm/kg NaCl or sucrose solutions	363:411	0, 100, and 300 mOsm/kg NaCl or sucrose solutions	363:411	Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5.
24718964	5	42	theme	effect	657:662	arg1	investigation					636:648	investigation	636:648	investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+)	636:731	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	1	43	theme	Salvelinus	240:249	arg1	fontinalis					251:260	brook trout Salvelinus fontinalis	228:260	brook trout Salvelinus fontinalis	228:260	The role of environmental ion composition and osmolality in Ca(2+) signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis.
24718964	7	44	theme	P	1017:1017	arg1	motility					1007:1014	significantly increased motility	983:1014	significantly increased motility (P < 0.05)	983:1025	Addition of amiloride led to significantly increased motility (P < 0.05) compared with sucrose (Na(+) free) solutions containing 2 mM EGTA.
24718964	7	44	theme	P	1017:1017	arg1	<					1019:1019	P < 0.05	1017:1024	P < 0.05	1017:1024	Addition of amiloride led to significantly increased motility (P < 0.05) compared with sucrose (Na(+) free) solutions containing 2 mM EGTA.
24718964	3	45	theme	NaCl	387:390	arg1	solutions					403:411	0, 100, and 300 mOsm/kg NaCl or sucrose solutions	363:411	0, 100, and 300 mOsm/kg NaCl or sucrose solutions	363:411	Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5.
24718964	9	46	theme	Ca	1254:1255	arg1	transport					1261:1269	Ca(2+) transport	1254:1269	Ca(2+) transport	1254:1269	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	4	47	theme	Ca	503:504	arg1	concentration					510:522	external Ca(2+) concentration	494:522	external Ca(2+) concentration	494:522	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	3	48	dep	mOsm/kg	379:385	arg1	100					366:368	100	366:368	100	366:368	Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5.
24718964	3	48	dep	mOsm/kg	379:385	arg1	300					375:377	300	375:377	300	375:377	Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5.
24718964	9	49	theme	transport	1261:1269	arg1	influence					1219:1227	The influence	1215:1227	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation	1215:1310	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	4	50	theme	spermatozoa	470:480	arg1	reaction					482:489	spermatozoa reaction	470:489	spermatozoa reaction	470:489	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	3	51	theme	mM	429:430	arg1	pH					441:442	10 mM Tris-HCl pH 8.5	426:446	10 mM Tris-HCl pH 8.5	426:446	Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5.
24718964	2	52	from	males	284:288	arg1	Milt					263:266	Milt	263:266	Milt from ten mature males	263:288	Milt from ten mature males was obtained by abdominal massage.
24718964	6	53	theme	Na	916:917	arg1	solutions					922:930	Na(+) solutions	916:930	Na(+) solutions containing 2 mM EGTA	916:951	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	4	54	theme	ethylene	530:537	arg1	glycol					539:544	2 mM ethylene glycol	525:544	2 mM ethylene glycol tetraacetic acid (EGTA)	525:568	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	5	55	theme	low	713:715	arg1	2+					729:730	2+	729:730	2+	729:730	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	5	55	theme	low	713:715	arg1	Ca					726:727	low external Ca	713:727	low external Ca(2+)	713:731	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	9	56	theme	activation	1301:1310	arg1	control					1278:1284	the control	1274:1284	the control of spermatozoa activation	1274:1310	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	4	57	theme	tetraacetic	546:556	arg1	chelator					622:629	a calcium ions chelator	607:629	a calcium ions chelator	607:629	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	4	57	theme	tetraacetic	546:556	arg1	EGTA					564:567	EGTA	564:567	EGTA	564:567	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	4	57	theme	tetraacetic	546:556	arg1	acid					558:561	2 mM ethylene glycol tetraacetic acid	525:561	2 mM ethylene glycol tetraacetic acid (EGTA)	525:568	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	1	58	theme	ion	136:138	arg1	composition					140:150	environmental ion composition	122:150	environmental ion composition	122:150	The role of environmental ion composition and osmolality in Ca(2+) signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis.
24718964	9	59	theme	further	1321:1327	arg1	study					1329:1333	further study	1321:1333	further study with respect to its application for improvement of artificial activation and storage media	1321:1424	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	5	60	theme	external	717:724	arg1	2+					729:730	2+	729:730	2+	729:730	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	5	60	theme	external	717:724	arg1	Ca					726:727	low external Ca	713:727	low external Ca(2+)	713:731	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	5	61	theme	concentration	682:694	arg1	effect					657:662	the effect	653:662	the effect of external Na(+) concentration in conditions of low external Ca(2+)	653:731	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	4	62	theme	2	525:525	arg1	mM					527:528	mM	527:528	mM	527:528	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	0	63	theme	spermatozoa	88:98	arg1	motility					100:107	brook trout (Salvelinus fontinalis) spermatozoa motility	52:107	brook trout (Salvelinus fontinalis) spermatozoa motility	52:107	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.
24718964	5	64	theme	µM	738:739	arg1	blocker					815:821	a Na(+) transport blocker	797:821	a Na(+) transport blocker	797:821	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	5	64	theme	µM	738:739	arg1	amiloride					741:749	100 µM amiloride	734:749	100 µM amiloride	734:749	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	6	65	theme	Low	824:826	arg1	motility					828:835	Low motility	824:835	Low motility	824:835	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	5	66	theme	EGTA-containing	768:782	arg1	solutions					784:792	the EGTA-containing solutions	764:792	the EGTA-containing solutions	764:792	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	5	67	theme	Ca	726:727	arg1	conditions					699:708	conditions	699:708	conditions of low external Ca(2+)	699:731	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	9	68	from	influence	1219:1227	arg1	control					1278:1284	the control	1274:1284	the control of spermatozoa activation	1274:1310	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	6	69	located	observed	841:848	arg2	motility					828:835	Low motility	824:835	Low motility	824:835	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	6	69	located	observed	841:848	arg1	solutions					874:882	sucrose (Na(+) free) solutions	853:882	sucrose (Na(+) free) solutions containing 2 mM EGTA	853:903	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	5	70	from	effect	657:662	arg1	conditions					699:708	conditions	699:708	conditions of low external Ca(2+)	699:731	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	1	71	from	role	114:117	arg1	2+					173:174	2+	173:174	2+	173:174	The role of environmental ion composition and osmolality in Ca(2+) signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis.
24718964	1	71	from	role	114:117	arg1	Ca					170:171	Ca	170:171	Ca(2+) signaled activation	170:195	The role of environmental ion composition and osmolality in Ca(2+) signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis.
24718964	9	72	theme	artificial	1386:1395	arg1	activation					1397:1406	artificial activation	1386:1406	artificial activation	1386:1406	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	0	73	from	role	4:7	arg1	motility					100:107	brook trout (Salvelinus fontinalis) spermatozoa motility	52:107	brook trout (Salvelinus fontinalis) spermatozoa motility	52:107	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.
24718964	0	74	theme	transport	39:47	arg1	role					4:7	The role	0:7	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.	0:108	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.
24718964	3	75	dep	NaCl	387:390	arg1	mOsm/kg					379:385	mOsm/kg	379:385	mOsm/kg	379:385	Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5.
24718964	4	76	theme	ions	617:620	arg1	chelator					622:629	a calcium ions chelator	607:629	a calcium ions chelator	607:629	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	4	76	theme	ions	617:620	arg1	acid					558:561	2 mM ethylene glycol tetraacetic acid	525:561	2 mM ethylene glycol tetraacetic acid (EGTA)	525:568	For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator.
24718964	0	77	theme	brook	52:56	arg1	trout					58:62	brook trout	52:62	brook trout (Salvelinus fontinalis) spermatozoa motility	52:107	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.
24718964	0	77	theme	brook	52:56	arg1	fontinalis					76:85	Salvelinus fontinalis	65:85	Salvelinus fontinalis	65:85	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.
24718964	9	78	theme	media	1420:1424	arg1	improvement					1371:1381	improvement	1371:1381	improvement of artificial activation and storage media	1371:1424	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	8	79	theme	brook	1179:1183	arg1	activation					1203:1212	brook trout spermatozoa activation	1179:1212	brook trout spermatozoa activation	1179:1212	We conclude that Na(+) transport in Ca(2+)-free solutions plays a regulatory role in brook trout spermatozoa activation.
24718964	8	80	theme	spermatozoa	1191:1201	arg1	activation					1203:1212	brook trout spermatozoa activation	1179:1212	brook trout spermatozoa activation	1179:1212	We conclude that Na(+) transport in Ca(2+)-free solutions plays a regulatory role in brook trout spermatozoa activation.
24718964	6	81	dep	sucrose	853:859	arg1	free					868:871	free	868:871	free	868:871	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	0	82	theme	Ca	12:13	arg1	role					4:7	The role	0:7	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.	0:108	The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility.
24718964	9	83	theme	Na	1244:1245	arg1	influence					1219:1227	The influence	1215:1227	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation	1215:1310	The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
24718964	1	84	theme	trout	234:238	arg1	fontinalis					251:260	brook trout Salvelinus fontinalis	228:260	brook trout Salvelinus fontinalis	228:260	The role of environmental ion composition and osmolality in Ca(2+) signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis.
24718964	6	85	theme	mM	897:898	arg1	EGTA					900:903	2 mM EGTA	895:903	2 mM EGTA	895:903	Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA.
24718964	1	86	theme	fontinalis	251:260	arg1	spermatozoa					213:223	spermatozoa	213:223	spermatozoa of brook trout Salvelinus fontinalis	213:260	The role of environmental ion composition and osmolality in Ca(2+) signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis.
24718964	5	87	theme	Na	799:800	arg1	blocker					815:821	a Na(+) transport blocker	797:821	a Na(+) transport blocker	797:821	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	5	87	theme	Na	799:800	arg1	amiloride					741:749	100 µM amiloride	734:749	100 µM amiloride	734:749	For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker.
24718964	7	88	theme	increased	997:1005	arg1	motility					1007:1014	significantly increased motility	983:1014	significantly increased motility (P < 0.05)	983:1025	Addition of amiloride led to significantly increased motility (P < 0.05) compared with sucrose (Na(+) free) solutions containing 2 mM EGTA.
24718964	7	88	theme	increased	997:1005	arg1	<					1019:1019	P < 0.05	1017:1024	P < 0.05	1017:1024	Addition of amiloride led to significantly increased motility (P < 0.05) compared with sucrose (Na(+) free) solutions containing 2 mM EGTA.
24718964	8	89	theme	Na	1111:1112	arg1	transport					1117:1125	Na(+) transport	1111:1125	Na(+) transport in Ca(2+)-free solutions	1111:1150	We conclude that Na(+) transport in Ca(2+)-free solutions plays a regulatory role in brook trout spermatozoa activation.
24718964	3	90	theme	10	426:427	arg1	mM					429:430	mM	429:430	mM	429:430	Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5.
24523895	10	0	theme	desirable	1545:1553	arg1	fruits					1555:1560	more desirable fruits	1540:1560	more desirable fruits	1540:1560	These findings allow for consumer influence in the breeding of more desirable fruits and vegetables.
24523895	6	1	theme	perceptually	996:1007	arg1	compounds					1019:1027	perceptually impactful compounds	996:1027	perceptually impactful compounds from the larger mixture	996:1051	The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined.
24523895	9	2	theme	sugar	1462:1466	arg1	content					1468:1474	sugar content	1462:1474	sugar content	1462:1474	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	5	3	theme	flavor	782:787	arg1	intensity					789:797	flavor intensity	782:797	flavor intensity	782:797	Overall liking is most greatly influenced by sweetness and strawberry flavor intensity, which are undermined by environmental pressures that reduce sucrose and total volatile content.
24523895	6	4	theme	strawberry	935:944	arg1	varieties					946:954	commercial strawberry varieties	924:954	commercial strawberry varieties	924:954	The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined.
24523895	3	5	theme	strawberry	503:512	arg1	fruit					514:518	strawberry fruit	503:518	strawberry fruit using a psychophysics approach	503:549	Analyses identify fruit attributes influencing hedonics and sensory perception of strawberry fruit using a psychophysics approach.
24523895	9	6	from	effect	1398:1403	arg1	intensity					1428:1436	perceived sweetness intensity	1408:1436	perceived sweetness intensity of fruit independent of sugar content	1408:1474	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	6	7	theme	compounds	1019:1027	arg1	list					988:991	a list	986:991	a list of perceptually impactful compounds from the larger mixture	986:1051	The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined.
24523895	6	8	theme	commercial	924:933	arg1	varieties					946:954	commercial strawberry varieties	924:954	commercial strawberry varieties	924:954	The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined.
24523895	1	9	dep	strawberries	113:124	arg1	ananassa					138:145	ananassa	138:145	ananassa	138:145	Fresh strawberries (Fragaria x ananassa) are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor.
24523895	1	9	dep	strawberries	113:124	arg1	Fragaria					127:134	Fragaria	127:134	Fragaria	127:134	Fresh strawberries (Fragaria x ananassa) are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor.
24523895	4	10	theme	texture	595:601	arg1	liking					603:608	texture liking	595:608	texture liking	595:608	Sweetness intensity, flavor intensity, and texture liking are dependent on sugar concentrations, specific volatile compounds, and fruit firmness, respectively.
24523895	11	11	theme	natural	1697:1703	arg1	products					1718:1725	natural or processed products	1697:1725	natural or processed products	1697:1725	Also, this approach garners insights into fruit metabolomics, flavor chemistry, and a paradigm for enhancing liking of natural or processed products.
24523895	6	12	from	mixture	1045:1051	arg1	compounds					1019:1027	perceptually impactful compounds	996:1027	perceptually impactful compounds from the larger mixture	996:1051	The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined.
24523895	6	12	from	mixture	1045:1051	arg1	list					988:991	a list	986:991	a list of perceptually impactful compounds from the larger mixture	986:1051	The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined.
24523895	8	13	theme	them	1300:1303	arg1	them					1300:1303	them	1300:1303	them	1300:1303	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	8	13	theme	them	1300:1303	arg1	intensity					1277:1285	strawberry flavor intensity	1259:1285	strawberry flavor intensity	1259:1285	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	8	13	theme	them	1300:1303	arg1	one					1293:1295	one	1293:1295	one	1293:1295	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	1	14	theme	sweet	230:234	arg1	flavor					243:248	sweet fruity flavor	230:248	sweet fruity flavor	230:248	Fresh strawberries (Fragaria x ananassa) are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor.
24523895	8	15	theme	thirty-one	1185:1194	arg1	compounds					1205:1213	thirty-one volatile compounds	1185:1213	thirty-one volatile compounds	1185:1213	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	11	16	theme	processed	1708:1716	arg1	products					1718:1725	natural or processed products	1697:1725	natural or processed products	1697:1725	Also, this approach garners insights into fruit metabolomics, flavor chemistry, and a paradigm for enhancing liking of natural or processed products.
24523895	4	17	theme	sugar	627:631	arg1	concentrations					633:646	sugar concentrations	627:646	sugar concentrations	627:646	Sweetness intensity, flavor intensity, and texture liking are dependent on sugar concentrations, specific volatile compounds, and fruit firmness, respectively.
24523895	9	18	theme	content	1468:1474	arg1	independent					1447:1457	independent	1447:1457	independent	1447:1457	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	5	19	theme	total	872:876	arg1	content					887:893	total volatile content	872:893	total volatile content	872:893	Overall liking is most greatly influenced by sweetness and strawberry flavor intensity, which are undermined by environmental pressures that reduce sucrose and total volatile content.
24523895	0	20	theme	sensory	87:93	arg1	perception					95:104	sensory perception	87:104	sensory perception	87:104	Strawberry flavor: diverse chemical compositions, a seasonal influence, and effects on sensory perception.
24523895	0	21	from	compositions	36:47	arg1	perception					95:104	sensory perception	87:104	sensory perception	87:104	Strawberry flavor: diverse chemical compositions, a seasonal influence, and effects on sensory perception.
24523895	9	22	theme	independent	1447:1457	arg1	fruit					1441:1445	fruit	1441:1445	fruit independent of sugar content	1441:1474	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	3	23	theme	psychophysics	528:540	arg1	approach					542:549	a psychophysics approach	526:549	a psychophysics approach	526:549	Analyses identify fruit attributes influencing hedonics and sensory perception of strawberry fruit using a psychophysics approach.
24523895	2	24	theme	strawberry	358:367	arg1	fruit					369:373	biochemically diverse strawberry fruit	336:373	biochemically diverse strawberry fruit	336:373	In this study, genetic and environmentally induced variation is exploited to capture biochemically diverse strawberry fruit for metabolite profiling and consumer rating.
24523895	9	25	theme	volatile	1356:1363	arg1	compounds					1365:1373	individual volatile compounds	1345:1373	individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content	1345:1474	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	10	26	theme	consumer	1502:1509	arg1	influence					1511:1519	consumer influence	1502:1519	consumer influence	1502:1519	These findings allow for consumer influence in the breeding of more desirable fruits and vegetables.
24523895	2	27	theme	genetic	266:272	arg1	variation					302:310	genetic and environmentally induced variation	266:310	genetic and environmentally induced variation	266:310	In this study, genetic and environmentally induced variation is exploited to capture biochemically diverse strawberry fruit for metabolite profiling and consumer rating.
24523895	2	28	theme	diverse	350:356	arg1	fruit					369:373	biochemically diverse strawberry fruit	336:373	biochemically diverse strawberry fruit	336:373	In this study, genetic and environmentally induced variation is exploited to capture biochemically diverse strawberry fruit for metabolite profiling and consumer rating.
24523895	7	29	dep	significant	1126:1136	arg1	fits					1138:1141	fits	1138:1141	fits to strawberry flavor intensity	1138:1172	Particular esters, terpenes, and furans have the most significant fits to strawberry flavor intensity.
24523895	1	30	theme	fruity	236:241	arg1	flavor					243:248	sweet fruity flavor	230:248	sweet fruity flavor	230:248	Fresh strawberries (Fragaria x ananassa) are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor.
24523895	8	31	theme	strawberry	1259:1268	arg1	them					1300:1303	them	1300:1303	them	1300:1303	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	8	31	theme	strawberry	1259:1268	arg1	intensity					1277:1285	strawberry flavor intensity	1259:1285	strawberry flavor intensity	1259:1285	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	8	31	theme	strawberry	1259:1268	arg1	one					1293:1295	one	1293:1295	one	1293:1295	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	5	32	theme	volatile	878:885	arg1	content					887:893	total volatile content	872:893	total volatile content	872:893	Overall liking is most greatly influenced by sweetness and strawberry flavor intensity, which are undermined by environmental pressures that reduce sucrose and total volatile content.
24523895	8	33	theme	flavor	1270:1275	arg1	them					1300:1303	them	1300:1303	them	1300:1303	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	8	33	theme	flavor	1270:1275	arg1	intensity					1277:1285	strawberry flavor intensity	1259:1285	strawberry flavor intensity	1259:1285	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	8	33	theme	flavor	1270:1275	arg1	one					1293:1295	one	1293:1295	one	1293:1295	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	0	34	theme	diverse	19:25	arg1	compositions					36:47	diverse chemical compositions	19:47	diverse chemical compositions	19:47	Strawberry flavor: diverse chemical compositions, a seasonal influence, and effects on sensory perception.
24523895	4	35	theme	Sweetness	552:560	arg1	intensity					562:570	Sweetness intensity	552:570	Sweetness intensity	552:570	Sweetness intensity, flavor intensity, and texture liking are dependent on sugar concentrations, specific volatile compounds, and fruit firmness, respectively.
24523895	8	36	located	found	1219:1223	arg1	total					1178:1182	total	1178:1182	total	1178:1182	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	8	36	located	found	1219:1223	arg2	compounds					1205:1213	thirty-one volatile compounds	1185:1213	thirty-one volatile compounds	1185:1213	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	9	37	theme	perceived	1408:1416	arg1	intensity					1428:1436	perceived sweetness intensity	1408:1436	perceived sweetness intensity of fruit independent of sugar content	1408:1474	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	1	38	theme	characteristic	169:182	arg1	color					188:192	their characteristic red color	163:192	their characteristic red color	163:192	Fresh strawberries (Fragaria x ananassa) are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor.
24523895	2	39	theme	consumer	404:411	arg1	rating					413:418	consumer rating	404:418	consumer rating	404:418	In this study, genetic and environmentally induced variation is exploited to capture biochemically diverse strawberry fruit for metabolite profiling and consumer rating.
24523895	4	40	theme	flavor	573:578	arg1	intensity					580:588	flavor intensity	573:588	flavor intensity	573:588	Sweetness intensity, flavor intensity, and texture liking are dependent on sugar concentrations, specific volatile compounds, and fruit firmness, respectively.
24523895	5	41	theme	Overall	712:718	arg1	liking					720:725	Overall liking	712:725	Overall liking	712:725	Overall liking is most greatly influenced by sweetness and strawberry flavor intensity, which are undermined by environmental pressures that reduce sucrose and total volatile content.
24523895	1	42	theme	red	184:186	arg1	color					188:192	their characteristic red color	163:192	their characteristic red color	163:192	Fresh strawberries (Fragaria x ananassa) are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor.
24523895	2	43	theme	induced	294:300	arg1	variation					302:310	genetic and environmentally induced variation	266:310	genetic and environmentally induced variation	266:310	In this study, genetic and environmentally induced variation is exploited to capture biochemically diverse strawberry fruit for metabolite profiling and consumer rating.
24523895	5	44	dep	sweetness	757:765	arg1	intensity					789:797	flavor intensity	782:797	flavor intensity	782:797	Overall liking is most greatly influenced by sweetness and strawberry flavor intensity, which are undermined by environmental pressures that reduce sucrose and total volatile content.
24523895	4	45	theme	specific	649:656	arg1	compounds					667:675	specific volatile compounds	649:675	specific volatile compounds	649:675	Sweetness intensity, flavor intensity, and texture liking are dependent on sugar concentrations, specific volatile compounds, and fruit firmness, respectively.
24523895	7	46	contain	have	1112:1115	arg2	significant					1126:1136	significant	1126:1136	significant	1126:1136	Particular esters, terpenes, and furans have the most significant fits to strawberry flavor intensity.
24523895	7	46	contain	have	1112:1115	arg1	terpenes					1091:1098	terpenes	1091:1098	terpenes	1091:1098	Particular esters, terpenes, and furans have the most significant fits to strawberry flavor intensity.
24523895	7	46	contain	have	1112:1115	arg1	furans					1105:1110	furans	1105:1110	furans	1105:1110	Particular esters, terpenes, and furans have the most significant fits to strawberry flavor intensity.
24523895	7	46	contain	have	1112:1115	arg1	esters					1083:1088	Particular esters	1072:1088	Particular esters	1072:1088	Particular esters, terpenes, and furans have the most significant fits to strawberry flavor intensity.
24523895	2	47	theme	metabolite	379:388	arg1	profiling					390:398	metabolite profiling	379:398	metabolite profiling	379:398	In this study, genetic and environmentally induced variation is exploited to capture biochemically diverse strawberry fruit for metabolite profiling and consumer rating.
24523895	0	48	theme	chemical	27:34	arg1	compositions					36:47	diverse chemical compositions	19:47	diverse chemical compositions	19:47	Strawberry flavor: diverse chemical compositions, a seasonal influence, and effects on sensory perception.
24523895	9	49	theme	enhancing	1388:1396	arg1	effect					1398:1403	an enhancing effect	1385:1403	an enhancing effect on perceived sweetness intensity of fruit independent of sugar content	1385:1474	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	6	50	from	list	988:991	arg1	mixture					1045:1051	the larger mixture	1034:1051	the larger mixture	1034:1051	The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined.
24523895	0	51	from	influence	61:69	arg1	perception					95:104	sensory perception	87:104	sensory perception	87:104	Strawberry flavor: diverse chemical compositions, a seasonal influence, and effects on sensory perception.
24523895	5	52	theme	environmental	824:836	arg1	pressures					838:846	environmental pressures	824:846	environmental pressures that reduce sucrose and total volatile content	824:893	Overall liking is most greatly influenced by sweetness and strawberry flavor intensity, which are undermined by environmental pressures that reduce sucrose and total volatile content.
24523895	3	53	theme	fruit	439:443	arg1	attributes					445:454	fruit attributes	439:454	fruit attributes influencing hedonics and sensory perception of strawberry fruit using a psychophysics approach	439:549	Analyses identify fruit attributes influencing hedonics and sensory perception of strawberry fruit using a psychophysics approach.
24523895	7	54	theme	strawberry	1146:1155	arg1	intensity					1164:1172	strawberry flavor intensity	1146:1172	strawberry flavor intensity	1146:1172	Particular esters, terpenes, and furans have the most significant fits to strawberry flavor intensity.
24523895	10	55	theme	vegetables	1566:1575	arg1	breeding					1528:1535	the breeding	1524:1535	the breeding of more desirable fruits and vegetables	1524:1575	These findings allow for consumer influence in the breeding of more desirable fruits and vegetables.
24523895	11	56	theme	flavor	1640:1645	arg1	chemistry					1647:1655	flavor chemistry	1640:1655	flavor chemistry	1640:1655	Also, this approach garners insights into fruit metabolomics, flavor chemistry, and a paradigm for enhancing liking of natural or processed products.
24523895	6	57	theme	volatile	900:907	arg1	profiles					909:916	The volatile profiles	896:916	The volatile profiles among commercial strawberry varieties	896:954	The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined.
24523895	6	57	theme	volatile	900:907	arg1	complex					960:966	complex	960:966	complex	960:966	The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined.
24523895	3	58	theme	sensory	481:487	arg1	perception					489:498	sensory perception	481:498	sensory perception of strawberry fruit using a psychophysics approach	481:549	Analyses identify fruit attributes influencing hedonics and sensory perception of strawberry fruit using a psychophysics approach.
24523895	7	59	theme	flavor	1157:1162	arg1	intensity					1164:1172	strawberry flavor intensity	1146:1172	strawberry flavor intensity	1146:1172	Particular esters, terpenes, and furans have the most significant fits to strawberry flavor intensity.
24523895	7	60	theme	Particular	1072:1081	arg1	esters					1083:1088	Particular esters	1072:1088	Particular esters	1072:1088	Particular esters, terpenes, and furans have the most significant fits to strawberry flavor intensity.
24523895	6	61	theme	impactful	1009:1017	arg1	compounds					1019:1027	perceptually impactful compounds	996:1027	perceptually impactful compounds from the larger mixture	996:1051	The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined.
24523895	11	62	theme	fruit	1620:1624	arg1	metabolomics					1626:1637	fruit metabolomics	1620:1637	fruit metabolomics	1620:1637	Also, this approach garners insights into fruit metabolomics, flavor chemistry, and a paradigm for enhancing liking of natural or processed products.
24523895	1	63	theme	Fresh	107:111	arg1	strawberries					113:124	Fresh strawberries	107:124	Fresh strawberries (Fragaria x ananassa)	107:146	Fresh strawberries (Fragaria x ananassa) are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor.
24523895	9	64	theme	individual	1345:1354	arg1	compounds					1365:1373	individual volatile compounds	1345:1373	individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content	1345:1474	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	10	65	theme	fruits	1555:1560	arg1	breeding					1528:1535	the breeding	1524:1535	the breeding of more desirable fruits and vegetables	1524:1575	These findings allow for consumer influence in the breeding of more desirable fruits and vegetables.
24523895	9	66	theme	sweetness	1418:1426	arg1	intensity					1428:1436	perceived sweetness intensity	1408:1436	perceived sweetness intensity of fruit independent of sugar content	1408:1474	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	1	67	theme	juicy	195:199	arg1	texture					201:207	juicy texture	195:207	juicy texture	195:207	Fresh strawberries (Fragaria x ananassa) are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor.
24523895	9	68	theme	Further	1317:1323	arg1	analysis					1325:1332	Further analysis	1317:1332	Further analysis	1317:1332	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	8	69	theme	volatile	1196:1203	arg1	compounds					1205:1213	thirty-one volatile compounds	1185:1213	thirty-one volatile compounds	1185:1213	In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively.
24523895	0	70	from	effects	76:82	arg1	perception					95:104	sensory perception	87:104	sensory perception	87:104	Strawberry flavor: diverse chemical compositions, a seasonal influence, and effects on sensory perception.
24523895	11	71	theme	products	1718:1725	arg1	liking					1687:1692	liking	1687:1692	liking of natural or processed products	1687:1725	Also, this approach garners insights into fruit metabolomics, flavor chemistry, and a paradigm for enhancing liking of natural or processed products.
24523895	3	72	theme	fruit	514:518	arg1	hedonics					468:475	hedonics	468:475	hedonics	468:475	Analyses identify fruit attributes influencing hedonics and sensory perception of strawberry fruit using a psychophysics approach.
24523895	3	72	theme	fruit	514:518	arg1	perception					489:498	sensory perception	481:498	sensory perception of strawberry fruit using a psychophysics approach	481:549	Analyses identify fruit attributes influencing hedonics and sensory perception of strawberry fruit using a psychophysics approach.
24523895	4	73	theme	volatile	658:665	arg1	compounds					667:675	specific volatile compounds	649:675	specific volatile compounds	649:675	Sweetness intensity, flavor intensity, and texture liking are dependent on sugar concentrations, specific volatile compounds, and fruit firmness, respectively.
24523895	6	74	theme	larger	1038:1043	arg1	mixture					1045:1051	the larger mixture	1034:1051	the larger mixture	1034:1051	The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined.
24523895	0	75	theme	seasonal	52:59	arg1	influence					61:69	a seasonal influence	50:69	a seasonal influence	50:69	Strawberry flavor: diverse chemical compositions, a seasonal influence, and effects on sensory perception.
24523895	9	76	contain	have	1380:1383	arg1	compounds					1365:1373	individual volatile compounds	1345:1373	individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content	1345:1474	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	9	76	contain	have	1380:1383	arg2	effect					1398:1403	an enhancing effect	1385:1403	an enhancing effect on perceived sweetness intensity of fruit independent of sugar content	1385:1474	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	9	77	theme	fruit	1441:1445	arg1	intensity					1428:1436	perceived sweetness intensity	1408:1436	perceived sweetness intensity of fruit independent of sugar content	1408:1474	Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content.
24523895	4	78	theme	fruit	682:686	arg1	firmness					688:695	fruit firmness	682:695	fruit firmness	682:695	Sweetness intensity, flavor intensity, and texture liking are dependent on sugar concentrations, specific volatile compounds, and fruit firmness, respectively.
24523895	1	79	theme	distinct	210:217	arg1	aroma					219:223	distinct aroma	210:223	distinct aroma	210:223	Fresh strawberries (Fragaria x ananassa) are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor.
24433701	1	0	from	minimization	155:166	arg1	separations					198:208	chiral separations	191:208	chiral separations	191:208	Application to the minimization of the solvent cost in chiral separations.
24433701	5	1	theme	experimental	854:865	arg1	results					867:873	The experimental results	850:873	The experimental results	850:873	The experimental results show that the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP are three times larger with acetonitrile than with methanol as the organic modifier.
24433701	4	2	theme	eddy	643:646	arg1	contributions					577:589	The five mass-transfer contributions	554:589	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP)	554:833	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	2	theme	eddy	643:646	arg1	dispersion					648:657	short-range and long-range eddy dispersion	616:657	short-range and long-range eddy dispersion	616:657	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	8	3	theme	long	1361:1364	arg1	column					1366:1371	a 5cm long column	1355:1371	a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column	1355:1443	This showed that analysis cost could be reduced seven times by selecting pure methanol as the eluent for a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column.
24433701	0	4	theme	transfer	69:76	arg1	mechanism					78:86	the mass transfer mechanism	60:86	the mass transfer mechanism in chiral reversed phase liquid chromatography	60:133	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	0	5	from	Impact	0:5	arg1	mechanism					78:86	the mass transfer mechanism	60:86	the mass transfer mechanism in chiral reversed phase liquid chromatography	60:133	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	2	6	theme	cellulose-based	259:273	arg1	phase					293:297	a cellulose-based chiral stationary phase	257:297	a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1)	257:320	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	3	7	dep	protocol	452:459	arg1	validated					461:469	validated	461:469	validated in RPLC and HILIC chromatography	461:502	An experimental protocol validated in RPLC and HILIC chromatography and recently extended to chiral RPLC was applied.
24433701	3	7	dep	protocol	452:459	arg1	extended					517:524	extended	517:524	recently extended to chiral RPLC	508:539	An experimental protocol validated in RPLC and HILIC chromatography and recently extended to chiral RPLC was applied.
24433701	0	8	theme	mass	64:67	arg1	mechanism					78:86	the mass transfer mechanism	60:86	the mass transfer mechanism in chiral reversed phase liquid chromatography	60:133	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	5	9	theme	enantiomers	938:948	arg1	constants					905:913	the adsorption rate constants	885:913	the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP	885:961	The experimental results show that the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP are three times larger with acetonitrile than with methanol as the organic modifier.
24433701	5	9	theme	enantiomers	938:948	arg1	larger					979:984	larger	979:984	larger	979:984	The experimental results show that the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP are three times larger with acetonitrile than with methanol as the organic modifier.
24433701	4	10	theme	intra-particle	713:726	arg1	diffusivity					728:738	finite intra-particle diffusivity	706:738	finite intra-particle diffusivity	706:738	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	11	theme	mass	673:676	arg1	resistances					687:697	solid-liquid mass transfer resistances	660:697	solid-liquid mass transfer resistances due to finite intra-particle diffusivity	660:738	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	8	12	theme	pure	1323:1326	arg1	methanol					1328:1335	pure methanol	1323:1335	pure methanol	1323:1335	This showed that analysis cost could be reduced seven times by selecting pure methanol as the eluent for a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column.
24433701	8	12	theme	pure	1323:1326	arg1	eluent					1344:1349	the eluent	1340:1349	the eluent for a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column	1340:1443	This showed that analysis cost could be reduced seven times by selecting pure methanol as the eluent for a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column.
24433701	4	13	theme	theoretical	810:820	arg1	plate					822:826	a theoretical plate	808:826	a theoretical plate (HETP)	808:833	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	13	theme	theoretical	810:820	arg1	HETP					829:832	HETP	829:832	HETP	829:832	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	14	theme	slow	744:747	arg1	contributions					577:589	The five mass-transfer contributions	554:589	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP)	554:833	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	14	theme	slow	744:747	arg1	adsorption-desorption					749:769	slow adsorption-desorption	744:769	slow adsorption-desorption	744:769	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	15	theme	short-range	616:626	arg1	contributions					577:589	The five mass-transfer contributions	554:589	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP)	554:833	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	15	theme	short-range	616:626	arg1	dispersion					648:657	short-range and long-range eddy dispersion	616:657	short-range and long-range eddy dispersion	616:657	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	8	16	theme	acetonitrile-water	1388:1405	arg1	mixture					1407:1413	an acetonitrile-water mixture	1385:1413	a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column	1355:1443	This showed that analysis cost could be reduced seven times by selecting pure methanol as the eluent for a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column.
24433701	5	17	theme	rate	900:903	arg1	constants					905:913	the adsorption rate constants	885:913	the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP	885:961	The experimental results show that the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP are three times larger with acetonitrile than with methanol as the organic modifier.
24433701	5	17	theme	rate	900:903	arg1	larger					979:984	larger	979:984	larger	979:984	The experimental results show that the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP are three times larger with acetonitrile than with methanol as the organic modifier.
24433701	6	18	from	1.1	1136:1138	arg1	acetonitrile					1144:1155	acetonitrile	1144:1155	acetonitrile	1144:1155	This is correlated to the decrease of enantioselectivity from 1.4 (in methanol) to only 1.1 (in acetonitrile).
24433701	4	19	theme	longitudinal	592:603	arg1	contributions					577:589	The five mass-transfer contributions	554:589	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP)	554:833	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	19	theme	longitudinal	592:603	arg1	diffusion					605:613	longitudinal diffusion	592:613	longitudinal diffusion	592:613	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	20	theme	finite	706:711	arg1	diffusivity					728:738	finite intra-particle diffusivity	706:738	finite intra-particle diffusivity	706:738	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	7	21	theme	separation	1201:1210	arg1	factor					1212:1217	a separation factor	1199:1217	a separation factor of exactly 2.0	1199:1232	The amount of solvent needed to achieve a separation factor of exactly 2.0 was determined.
24433701	2	22	theme	acetonitrile	348:359	arg1	phase					429:433	the mobile phase	418:433	the mobile phase	418:433	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	2	22	theme	acetonitrile	348:359	arg1	mixtures					336:343	aqueous mixtures	328:343	aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v)	328:413	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	2	23	dep	90/10	393:397	arg1	v/v					410:412	v/v	410:412	v/v	410:412	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	8	24	theme	5cm	1357:1359	arg1	column					1366:1371	a 5cm long column	1355:1371	a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column	1355:1443	This showed that analysis cost could be reduced seven times by selecting pure methanol as the eluent for a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column.
24433701	0	25	theme	nature	14:19	arg1	Impact					0:5	Impact	0:5	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography	0:133	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	2	26	dep	50/50-90/10	362:372	arg1	v/v					375:377	v/v	375:377	v/v	375:377	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	0	27	theme	reversed	98:105	arg1	chromatography					120:133	chiral reversed phase liquid chromatography	91:133	chiral reversed phase liquid chromatography	91:133	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	5	28	theme	adsorption	889:898	arg1	constants					905:913	the adsorption rate constants	885:913	the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP	885:961	The experimental results show that the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP are three times larger with acetonitrile than with methanol as the organic modifier.
24433701	5	28	theme	adsorption	889:898	arg1	larger					979:984	larger	979:984	larger	979:984	The experimental results show that the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP are three times larger with acetonitrile than with methanol as the organic modifier.
24433701	2	29	theme	stationary	282:291	arg1	phase					293:297	a cellulose-based chiral stationary phase	257:297	a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1)	257:320	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	6	30	from	1.4	1110:1112	arg1	methanol					1118:1125	methanol	1118:1125	methanol	1118:1125	This is correlated to the decrease of enantioselectivity from 1.4 (in methanol) to only 1.1 (in acetonitrile).
24433701	6	30	from	1.4	1110:1112	arg1	decrease					1074:1081	the decrease	1070:1081	the decrease of enantioselectivity from 1.4 (in methanol) to only 1.1 (in acetonitrile)	1070:1156	This is correlated to the decrease of enantioselectivity from 1.4 (in methanol) to only 1.1 (in acetonitrile).
24433701	1	31	theme	solvent	175:181	arg1	cost					183:186	the solvent cost	171:186	the solvent cost	171:186	Application to the minimization of the solvent cost in chiral separations.
24433701	2	32	theme	methanol	383:390	arg1	phase					429:433	the mobile phase	418:433	the mobile phase	418:433	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	2	32	theme	methanol	383:390	arg1	mixtures					336:343	aqueous mixtures	328:343	aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v)	328:413	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	0	33	theme	chiral	91:96	arg1	chromatography					120:133	chiral reversed phase liquid chromatography	91:133	chiral reversed phase liquid chromatography	91:133	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	5	34	theme	organic	1030:1036	arg1	modifier					1038:1045	the organic modifier	1026:1045	the organic modifier	1026:1045	The experimental results show that the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP are three times larger with acetonitrile than with methanol as the organic modifier.
24433701	2	35	theme	chiral	275:280	arg1	phase					293:297	a cellulose-based chiral stationary phase	257:297	a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1)	257:320	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	1	36	theme	cost	183:186	arg1	minimization					155:166	the minimization	151:166	the minimization of the solvent cost in chiral separations	151:208	Application to the minimization of the solvent cost in chiral separations.
24433701	0	37	theme	composition	25:35	arg1	Impact					0:5	Impact	0:5	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography	0:133	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	5	38	theme	trans-stilbene	923:936	arg1	enantiomers					938:948	trans-stilbene enantiomers	923:948	trans-stilbene enantiomers	923:948	The experimental results show that the adsorption rate constants kads of trans-stilbene enantiomers onto the CSP are three times larger with acetonitrile than with methanol as the organic modifier.
24433701	0	39	theme	liquid	113:118	arg1	chromatography					120:133	chiral reversed phase liquid chromatography	91:133	chiral reversed phase liquid chromatography	91:133	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	6	40	theme	enantioselectivity	1086:1103	arg1	decrease					1074:1081	the decrease	1070:1081	the decrease of enantioselectivity from 1.4 (in methanol) to only 1.1 (in acetonitrile)	1070:1156	This is correlated to the decrease of enantioselectivity from 1.4 (in methanol) to only 1.1 (in acetonitrile).
24433701	3	41	theme	chiral	529:534	arg1	RPLC					536:539	chiral RPLC	529:539	chiral RPLC	529:539	An experimental protocol validated in RPLC and HILIC chromatography and recently extended to chiral RPLC was applied.
24433701	4	42	theme	long-range	632:641	arg1	contributions					577:589	The five mass-transfer contributions	554:589	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP)	554:833	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	42	theme	long-range	632:641	arg1	dispersion					648:657	short-range and long-range eddy dispersion	616:657	short-range and long-range eddy dispersion	616:657	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	0	43	theme	phase	107:111	arg1	chromatography					120:133	chiral reversed phase liquid chromatography	91:133	chiral reversed phase liquid chromatography	91:133	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	4	44	theme	reduced	779:785	arg1	height					787:792	the reduced height	775:792	the reduced height equivalent to a theoretical plate (HETP)	775:833	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	1	45	theme	chiral	191:196	arg1	separations					198:208	chiral separations	191:208	chiral separations	191:208	Application to the minimization of the solvent cost in chiral separations.
24433701	2	46	theme	mobile	422:427	arg1	phase					429:433	the mobile phase	418:433	the mobile phase	418:433	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	2	46	theme	mobile	422:427	arg1	mixtures					336:343	aqueous mixtures	328:343	aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v)	328:413	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	4	47	theme	solid-liquid	660:671	arg1	resistances					687:697	solid-liquid mass transfer resistances	660:697	solid-liquid mass transfer resistances due to finite intra-particle diffusivity	660:738	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	8	48	theme	longer	1421:1426	arg1	column					1438:1443	a longer (20-45cm) column	1419:1443	a longer (20-45cm) column	1419:1443	This showed that analysis cost could be reduced seven times by selecting pure methanol as the eluent for a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column.
24433701	4	49	theme	equivalent	794:803	arg1	height					787:792	the reduced height	775:792	the reduced height equivalent to a theoretical plate (HETP)	775:833	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	2	50	theme	Lux	305:307	arg1	CSP					300:302	CSP	300:302	CSP	300:302	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	2	50	theme	Lux	305:307	arg1	Cellulose-1					309:319	Lux Cellulose-1	305:319	Lux Cellulose-1	305:319	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	0	51	from	mechanism	78:86	arg1	chromatography					120:133	chiral reversed phase liquid chromatography	91:133	chiral reversed phase liquid chromatography	91:133	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	0	52	dep	nature	14:19	arg1	the					10:12	the	10:12	the	10:12	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	4	53	dep	contributions	577:589	arg1	contributions					577:589	The five mass-transfer contributions	554:589	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP)	554:833	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	53	dep	contributions	577:589	arg1	diffusion					605:613	longitudinal diffusion	592:613	longitudinal diffusion	592:613	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	53	dep	contributions	577:589	arg1	resistances					687:697	solid-liquid mass transfer resistances	660:697	solid-liquid mass transfer resistances due to finite intra-particle diffusivity	660:738	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	53	dep	contributions	577:589	arg1	dispersion					648:657	short-range and long-range eddy dispersion	616:657	short-range and long-range eddy dispersion	616:657	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	53	dep	contributions	577:589	arg1	adsorption-desorption					749:769	slow adsorption-desorption	744:769	slow adsorption-desorption	744:769	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	54	theme	transfer	678:685	arg1	resistances					687:697	solid-liquid mass transfer resistances	660:697	solid-liquid mass transfer resistances due to finite intra-particle diffusivity	660:738	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	3	55	theme	HILIC	483:487	arg1	chromatography					489:502	HILIC chromatography	483:502	HILIC chromatography	483:502	An experimental protocol validated in RPLC and HILIC chromatography and recently extended to chiral RPLC was applied.
24433701	2	56	theme	transfer	233:240	arg1	mechanism					215:223	The mechanism	211:223	The mechanism of mass transfer	211:240	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	2	57	dep	phase	293:297	arg1	CSP					300:302	CSP	300:302	CSP	300:302	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	2	57	dep	phase	293:297	arg1	Cellulose-1					309:319	Lux Cellulose-1	305:319	Lux Cellulose-1	305:319	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	8	58	theme	analysis	1267:1274	arg1	cost					1276:1279	analysis cost	1267:1279	analysis cost	1267:1279	This showed that analysis cost could be reduced seven times by selecting pure methanol as the eluent for a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column.
24433701	0	59	theme	phase	51:55	arg1	composition					25:35	composition	25:35	composition	25:35	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	0	59	theme	phase	51:55	arg1	nature					14:19	nature	14:19	nature	14:19	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	7	60	theme	2.0	1230:1232	arg1	factor					1212:1217	a separation factor	1199:1217	a separation factor of exactly 2.0	1199:1232	The amount of solvent needed to achieve a separation factor of exactly 2.0 was determined.
24433701	2	61	theme	mass	228:231	arg1	transfer					233:240	mass transfer	228:240	mass transfer	228:240	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	4	62	theme	mass-transfer	563:575	arg1	contributions					577:589	The five mass-transfer contributions	554:589	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP)	554:833	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	62	theme	mass-transfer	563:575	arg1	diffusion					605:613	longitudinal diffusion	592:613	longitudinal diffusion	592:613	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	62	theme	mass-transfer	563:575	arg1	adsorption-desorption					749:769	slow adsorption-desorption	744:769	slow adsorption-desorption	744:769	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	4	62	theme	mass-transfer	563:575	arg1	dispersion					648:657	short-range and long-range eddy dispersion	616:657	short-range and long-range eddy dispersion	616:657	The five mass-transfer contributions (longitudinal diffusion, short-range and long-range eddy dispersion, solid-liquid mass transfer resistances due to finite intra-particle diffusivity and slow adsorption-desorption) to the reduced height equivalent to a theoretical plate (HETP) were measured.
24433701	3	63	theme	experimental	439:450	arg1	protocol					452:459	An experimental protocol	436:459	An experimental protocol validated in RPLC and HILIC chromatography and recently extended to chiral RPLC	436:539	An experimental protocol validated in RPLC and HILIC chromatography and recently extended to chiral RPLC was applied.
24433701	6	64	dep	1.4	1110:1112	arg1	to					1128:1129	to	1128:1129	to	1128:1129	This is correlated to the decrease of enantioselectivity from 1.4 (in methanol) to only 1.1 (in acetonitrile).
24433701	6	64	dep	1.4	1110:1112	arg1	1.1					1136:1138	1.1	1136:1138	1.1	1136:1138	This is correlated to the decrease of enantioselectivity from 1.4 (in methanol) to only 1.1 (in acetonitrile).
24433701	0	65	theme	mobile	44:49	arg1	phase					51:55	the mobile phase	40:55	the mobile phase	40:55	Impact of the nature and composition of the mobile phase on the mass transfer mechanism in chiral reversed phase liquid chromatography.
24433701	8	66	dep	longer	1421:1426	arg1	20-45cm					1429:1435	20-45cm	1429:1435	20-45cm	1429:1435	This showed that analysis cost could be reduced seven times by selecting pure methanol as the eluent for a 5cm long column rather than an acetonitrile-water mixture for a longer (20-45cm) column.
24433701	2	67	theme	aqueous	328:334	arg1	phase					429:433	the mobile phase	418:433	the mobile phase	418:433	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	2	67	theme	aqueous	328:334	arg1	mixtures					336:343	aqueous mixtures	328:343	aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v)	328:413	The mechanism of mass transfer was studied on a cellulose-based chiral stationary phase (CSP, Lux Cellulose-1) using aqueous mixtures of acetonitrile (50/50-90/10, v/v) or methanol (90/10 and 100/0, v/v) as the mobile phase.
24433701	7	68	theme	solvent	1173:1179	arg1	solvent					1173:1179	solvent	1173:1179	solvent	1173:1179	The amount of solvent needed to achieve a separation factor of exactly 2.0 was determined.
24433701	7	68	theme	solvent	1173:1179	arg1	amount					1163:1168	The amount	1159:1168	The amount of solvent needed to achieve a separation factor of exactly 2.0	1159:1232	The amount of solvent needed to achieve a separation factor of exactly 2.0 was determined.
24124012	10	0	with	lactobacilli	1084:1095	arg1	OFS					1113:1115	OFS	1113:1115	OFS that was not observed with probiotic alone	1113:1158	There was a marked increase in bifidobacteria and lactobacilli (P < 0.01) with OFS that was not observed with probiotic alone.
24124012	11	1	theme	probiotic	1277:1285	arg1	BB-12					1287:1291	the probiotic BB-12	1273:1291	the probiotic BB-12	1273:1291	CONCLUSIONS The impact of prebiotic intake on body composition and gut microbiota was of greater magnitude than the probiotic BB-12.
24124012	2	2	theme	Bifidobacterium	333:347	arg1	subsp					358:362	the probiotic Bifidobacterium animalis subsp	319:362	the probiotic Bifidobacterium animalis subsp	319:362	The aim of this study was to examine the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp.
24124012	11	3	theme	prebiotic	1187:1195	arg1	intake					1197:1202	prebiotic intake	1187:1202	prebiotic intake	1187:1202	CONCLUSIONS The impact of prebiotic intake on body composition and gut microbiota was of greater magnitude than the probiotic BB-12.
24124012	0	4	from	effects	9:15	arg1	glycemia					85:92	glycemia	85:92	glycemia	85:92	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats.
24124012	0	4	from	effects	9:15	arg1	microbiota					70:79	gut microbiota	66:79	gut microbiota	66:79	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats.
24124012	7	5	theme	P	877:877	arg1	<					879:879	P < 0.05	877:884	P < 0.05	877:884	OFS, BB-12, and the combined OFS + BB-12 improved glycemia (P < 0.05).
24124012	7	5	theme	P	877:877	arg1	OFS					846:848	the combined OFS	833:848	the combined OFS	833:848	OFS, BB-12, and the combined OFS + BB-12 improved glycemia (P < 0.05).
24124012	11	6	theme	body	1207:1210	arg1	composition					1212:1222	body composition	1207:1222	body composition	1207:1222	CONCLUSIONS The impact of prebiotic intake on body composition and gut microbiota was of greater magnitude than the probiotic BB-12.
24124012	2	7	theme	probiotic	323:331	arg1	subsp					358:362	the probiotic Bifidobacterium animalis subsp	319:362	the probiotic Bifidobacterium animalis subsp	319:362	The aim of this study was to examine the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp.
24124012	6	8	theme	fat	796:798	arg1	mass					800:803	fat mass	796:803	fat mass (P < 0.01)	796:814	RESULTS Prebiotic, but not probiotic reduced energy intake, weight gain, and fat mass (P < 0.01).
24124012	6	8	theme	fat	796:798	arg1	<					808:808	P < 0.01	806:813	P < 0.01	806:813	RESULTS Prebiotic, but not probiotic reduced energy intake, weight gain, and fat mass (P < 0.01).
24124012	0	9	theme	obese	97:101	arg1	rats					103:106	obese rats	97:106	obese rats	97:106	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats.
24124012	9	10	theme	increased	1006:1014	arg1	GLP-2					1016:1020	probiotic increased GLP-2	996:1020	probiotic increased GLP-2 (P < 0.05)	996:1031	Portal GLP-1 was increased with OFS, whereas probiotic increased GLP-2 (P < 0.05).
24124012	9	10	theme	increased	1006:1014	arg1	<					1025:1025	P < 0.05	1023:1030	P < 0.05	1023:1030	Portal GLP-1 was increased with OFS, whereas probiotic increased GLP-2 (P < 0.05).
24124012	7	11	theme	improved	858:865	arg1	glycemia					867:874	BB-12 improved glycemia	852:874	BB-12 improved glycemia	852:874	OFS, BB-12, and the combined OFS + BB-12 improved glycemia (P < 0.05).
24124012	12	12	theme	"	1428:1428	arg1	agents					1430:1435	these "biotic" agents	1415:1435	these "biotic" agents	1415:1435	Despite this, an improvement in glucose AUC with both prebiotic or probiotic demonstrates the beneficial role of each of these "biotic" agents in glycemic control.
24124012	12	13	theme	glycemic	1440:1447	arg1	control					1449:1455	glycemic control	1440:1455	glycemic control	1440:1455	Despite this, an improvement in glucose AUC with both prebiotic or probiotic demonstrates the beneficial role of each of these "biotic" agents in glycemic control.
24124012	6	14	theme	weight	779:784	arg1	gain					786:789	weight gain	779:789	weight gain	779:789	RESULTS Prebiotic, but not probiotic reduced energy intake, weight gain, and fat mass (P < 0.01).
24124012	4	15	theme	Adult	447:451	arg1	rats					493:496	Adult male, diet-induced obese Sprague Dawley rats	447:496	Adult male, diet-induced obese Sprague Dawley rats	447:496	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	11	16	from	impact	1177:1182	arg1	composition					1212:1222	body composition	1207:1222	body composition	1207:1222	CONCLUSIONS The impact of prebiotic intake on body composition and gut microbiota was of greater magnitude than the probiotic BB-12.
24124012	11	16	from	impact	1177:1182	arg1	microbiota					1232:1241	gut microbiota	1228:1241	gut microbiota	1228:1241	CONCLUSIONS The impact of prebiotic intake on body composition and gut microbiota was of greater magnitude than the probiotic BB-12.
24124012	11	17	theme	intake	1197:1202	arg1	impact					1177:1182	The impact	1173:1182	The impact of prebiotic intake on body composition and gut microbiota was of greater magnitude than the probiotic BB-12	1173:1291	CONCLUSIONS The impact of prebiotic intake on body composition and gut microbiota was of greater magnitude than the probiotic BB-12.
24124012	4	18	theme	=	590:590	arg1	rats/group					597:606	n = 9-10 rats/group	588:606	n = 9-10 rats/group	588:606	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	4	18	theme	=	590:590	arg1	OFS					563:565	(4) OFS	559:565	(4) OFS	559:565	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	12	19	theme	biotic	1422:1427	arg1	agents					1430:1435	these "biotic" agents	1415:1435	these "biotic" agents	1415:1435	Despite this, an improvement in glucose AUC with both prebiotic or probiotic demonstrates the beneficial role of each of these "biotic" agents in glycemic control.
24124012	2	20	theme	combined	261:268	arg1	effects					270:276	the individual and combined effects	242:276	the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp	242:362	The aim of this study was to examine the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp.
24124012	3	21	theme	host	408:411	arg1	metabolism					413:422	host metabolism	408:422	host metabolism	408:422	lactis BB-12 (BB-12) on gut microbiota and host metabolism in obese rats.
24124012	3	22	from	BB-12	372:376	arg1	microbiota					393:402	gut microbiota	389:402	gut microbiota	389:402	lactis BB-12 (BB-12) on gut microbiota and host metabolism in obese rats.
24124012	3	22	from	BB-12	372:376	arg1	metabolism					413:422	host metabolism	408:422	host metabolism	408:422	lactis BB-12 (BB-12) on gut microbiota and host metabolism in obese rats.
24124012	3	22	from	BB-12	372:376	arg1	rats					433:436	obese rats	427:436	obese rats	427:436	lactis BB-12 (BB-12) on gut microbiota and host metabolism in obese rats.
24124012	7	23	theme	BB-12	852:856	arg1	glycemia					867:874	BB-12 improved glycemia	852:874	BB-12 improved glycemia	852:874	OFS, BB-12, and the combined OFS + BB-12 improved glycemia (P < 0.05).
24124012	12	24	from	improvement	1311:1321	arg1	AUC					1334:1336	glucose AUC	1326:1336	glucose AUC	1326:1336	Despite this, an improvement in glucose AUC with both prebiotic or probiotic demonstrates the beneficial role of each of these "biotic" agents in glycemic control.
24124012	9	25	theme	Portal	951:956	arg1	GLP-1					958:962	Portal GLP-1	951:962	Portal GLP-1	951:962	Portal GLP-1 was increased with OFS, whereas probiotic increased GLP-2 (P < 0.05).
24124012	4	26	theme	male	453:456	arg1	rats					493:496	Adult male, diet-induced obese Sprague Dawley rats	447:496	Adult male, diet-induced obese Sprague Dawley rats	447:496	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	7	27	theme	combined	837:844	arg1	<					879:879	P < 0.05	877:884	P < 0.05	877:884	OFS, BB-12, and the combined OFS + BB-12 improved glycemia (P < 0.05).
24124012	7	27	theme	combined	837:844	arg1	OFS					846:848	the combined OFS	833:848	the combined OFS	833:848	OFS, BB-12, and the combined OFS + BB-12 improved glycemia (P < 0.05).
24124012	10	28	theme	<	1100:1100	arg1	P					1098:1098	P < 0.01	1098:1105	P < 0.01	1098:1105	There was a marked increase in bifidobacteria and lactobacilli (P < 0.01) with OFS that was not observed with probiotic alone.
24124012	10	28	theme	<	1100:1100	arg1	bifidobacteria					1065:1078	bifidobacteria	1065:1078	bifidobacteria	1065:1078	There was a marked increase in bifidobacteria and lactobacilli (P < 0.01) with OFS that was not observed with probiotic alone.
24124012	12	29	with	improvement	1311:1321	arg1	prebiotic					1348:1356	prebiotic	1348:1356	prebiotic	1348:1356	Despite this, an improvement in glucose AUC with both prebiotic or probiotic demonstrates the beneficial role of each of these "biotic" agents in glycemic control.
24124012	12	29	with	improvement	1311:1321	arg1	probiotic					1361:1369	probiotic	1361:1369	probiotic	1361:1369	Despite this, an improvement in glucose AUC with both prebiotic or probiotic demonstrates the beneficial role of each of these "biotic" agents in glycemic control.
24124012	12	30	theme	glucose	1326:1332	arg1	AUC					1334:1336	glucose AUC	1326:1336	glucose AUC	1326:1336	Despite this, an improvement in glucose AUC with both prebiotic or probiotic demonstrates the beneficial role of each of these "biotic" agents in glycemic control.
24124012	1	31	theme	obesity-associated	170:187	arg1	microbiota					193:202	an obesity-associated gut microbiota	167:202	an obesity-associated gut microbiota	167:202	OBJECTIVE Prebiotics and probiotics may be able to modify an obesity-associated gut microbiota.
24124012	0	32	theme	Combined	0:7	arg1	effects					9:15	Combined effects	0:15	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats	0:106	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats.
24124012	2	33	theme	oligofructose	295:307	arg1	effects					270:276	the individual and combined effects	242:276	the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp	242:362	The aim of this study was to examine the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp.
24124012	1	34	theme	gut	189:191	arg1	microbiota					193:202	an obesity-associated gut microbiota	167:202	an obesity-associated gut microbiota	167:202	OBJECTIVE Prebiotics and probiotics may be able to modify an obesity-associated gut microbiota.
24124012	0	35	theme	oligofructose	20:32	arg1	effects					9:15	Combined effects	0:15	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats	0:106	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats.
24124012	4	36	dep	male	453:456	arg1	diet-induced					459:470	diet-induced	459:470	diet-induced	459:470	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	11	37	theme	gut	1228:1230	arg1	microbiota					1232:1241	gut microbiota	1228:1241	gut microbiota	1228:1241	CONCLUSIONS The impact of prebiotic intake on body composition and gut microbiota was of greater magnitude than the probiotic BB-12.
24124012	2	38	theme	prebiotic	285:293	arg1	OFS					310:312	OFS	310:312	OFS	310:312	The aim of this study was to examine the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp.
24124012	2	38	theme	prebiotic	285:293	arg1	oligofructose					295:307	the prebiotic oligofructose	281:307	the prebiotic oligofructose (OFS)	281:313	The aim of this study was to examine the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp.
24124012	4	39	theme	%	541:541	arg1	OFS					543:545	10% OFS	539:545	(1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group)	518:607	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	3	40	theme	lactis	365:370	arg1	BB-12					372:376	lactis BB-12	365:376	lactis BB-12 (BB-12) on gut microbiota and host metabolism in obese rats.	365:437	lactis BB-12 (BB-12) on gut microbiota and host metabolism in obese rats.
24124012	3	40	theme	lactis	365:370	arg1	BB-12					379:383	BB-12	379:383	BB-12	379:383	lactis BB-12 (BB-12) on gut microbiota and host metabolism in obese rats.
24124012	0	41	from	glycemia	85:92	arg1	rats					103:106	obese rats	97:106	obese rats	97:106	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats.
24124012	4	42	dep	OFS	563:565	arg1	4					560:560	4	560:560	4	560:560	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	5	43	theme	satiety	656:662	arg1	hormones					664:671	satiety hormones	656:671	satiety hormones	656:671	Body composition, glycemia, gut permeability, satiety hormones, cytokines, and gut microbiota were examined.
24124012	0	44	theme	Bifidobacterium	38:52	arg1	animalis					54:61	Bifidobacterium animalis	38:61	Bifidobacterium animalis	38:61	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats.
24124012	4	45	dep	METHODS	439:445	arg1	randomized					503:512	randomized	503:512	were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group)	498:607	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	5	46	theme	gut	689:691	arg1	microbiota					693:702	gut microbiota	689:702	gut microbiota	689:702	Body composition, glycemia, gut permeability, satiety hormones, cytokines, and gut microbiota were examined.
24124012	10	47	from	increase	1053:1060	arg1	P					1098:1098	P < 0.01	1098:1105	P < 0.01	1098:1105	There was a marked increase in bifidobacteria and lactobacilli (P < 0.01) with OFS that was not observed with probiotic alone.
24124012	10	47	from	increase	1053:1060	arg1	lactobacilli					1084:1095	lactobacilli	1084:1095	lactobacilli	1084:1095	There was a marked increase in bifidobacteria and lactobacilli (P < 0.01) with OFS that was not observed with probiotic alone.
24124012	10	47	from	increase	1053:1060	arg1	bifidobacteria					1065:1078	bifidobacteria	1065:1078	bifidobacteria	1065:1078	There was a marked increase in bifidobacteria and lactobacilli (P < 0.01) with OFS that was not observed with probiotic alone.
24124012	3	48	theme	gut	389:391	arg1	microbiota					393:402	gut microbiota	389:402	gut microbiota	389:402	lactis BB-12 (BB-12) on gut microbiota and host metabolism in obese rats.
24124012	6	49	theme	energy	764:769	arg1	intake					771:776	energy intake	764:776	energy intake	764:776	RESULTS Prebiotic, but not probiotic reduced energy intake, weight gain, and fat mass (P < 0.01).
24124012	5	50	theme	Body	610:613	arg1	composition					615:625	Body composition	610:625	Body composition	610:625	Body composition, glycemia, gut permeability, satiety hormones, cytokines, and gut microbiota were examined.
24124012	0	51	from	microbiota	70:79	arg1	rats					103:106	obese rats	97:106	obese rats	97:106	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats.
24124012	2	52	theme	study	221:225	arg1	aim					209:211	The aim	205:211	The aim of this study	205:225	The aim of this study was to examine the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp.
24124012	4	53	theme	Dawley	486:491	arg1	rats					493:496	Adult male, diet-induced obese Sprague Dawley rats	447:496	Adult male, diet-induced obese Sprague Dawley rats	447:496	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	3	54	theme	obese	427:431	arg1	rats					433:436	obese rats	427:436	obese rats	427:436	lactis BB-12 (BB-12) on gut microbiota and host metabolism in obese rats.
24124012	4	55	dep	Control	522:528	arg1	BB-12					569:573	BB-12	569:573	BB-12 for 8 weeks	569:585	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	4	55	dep	Control	522:528	arg1	OFS					543:545	10% OFS	539:545	(1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group)	518:607	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	4	55	dep	Control	522:528	arg1	rats/group					597:606	n = 9-10 rats/group	588:606	n = 9-10 rats/group	588:606	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	4	55	dep	Control	522:528	arg1	1					519:519	1	519:519	1	519:519	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	4	55	dep	Control	522:528	arg1	OFS					563:565	(4) OFS	559:565	(4) OFS	559:565	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	4	55	dep	Control	522:528	arg1	BB-12					552:556	(3) BB-12	548:556	(1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group)	518:607	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	9	56	theme	P	1023:1023	arg1	GLP-2					1016:1020	probiotic increased GLP-2	996:1020	probiotic increased GLP-2 (P < 0.05)	996:1031	Portal GLP-1 was increased with OFS, whereas probiotic increased GLP-2 (P < 0.05).
24124012	9	56	theme	P	1023:1023	arg1	<					1025:1025	P < 0.05	1023:1030	P < 0.05	1023:1030	Portal GLP-1 was increased with OFS, whereas probiotic increased GLP-2 (P < 0.05).
24124012	1	57	theme	OBJECTIVE	109:117	arg1	Prebiotics					119:128	Prebiotics	119:128	Prebiotics	119:128	OBJECTIVE Prebiotics and probiotics may be able to modify an obesity-associated gut microbiota.
24124012	0	58	theme	animalis	54:61	arg1	effects					9:15	Combined effects	0:15	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats	0:106	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats.
24124012	6	59	theme	probiotic	746:754	arg1	RESULTS					719:725	RESULTS	719:725	RESULTS Prebiotic, but not probiotic	719:754	RESULTS Prebiotic, but not probiotic reduced energy intake, weight gain, and fat mass (P < 0.01).
24124012	6	60	theme	Prebiotic	727:735	arg1	RESULTS					719:725	RESULTS	719:725	RESULTS Prebiotic, but not probiotic	719:754	RESULTS Prebiotic, but not probiotic reduced energy intake, weight gain, and fat mass (P < 0.01).
24124012	5	61	theme	gut	638:640	arg1	permeability					642:653	gut permeability	638:653	gut permeability	638:653	Body composition, glycemia, gut permeability, satiety hormones, cytokines, and gut microbiota were examined.
24124012	8	62	theme	P	940:940	arg1	levels					932:937	insulin levels	924:937	insulin levels (P < 0.05)	924:948	Individually, OFS and BB-12 reduced insulin levels (P < 0.05).
24124012	8	62	theme	P	940:940	arg1	<					942:942	P < 0.05	940:947	P < 0.05	940:947	Individually, OFS and BB-12 reduced insulin levels (P < 0.05).
24124012	12	63	theme	each	1407:1410	arg1	role					1399:1402	the beneficial role	1384:1402	the beneficial role of each of these "biotic" agents in glycemic control	1384:1455	Despite this, an improvement in glucose AUC with both prebiotic or probiotic demonstrates the beneficial role of each of these "biotic" agents in glycemic control.
24124012	4	64	dep	BB-12	552:556	arg1	3					549:549	3	549:549	3	549:549	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	2	65	theme	individual	246:255	arg1	effects					270:276	the individual and combined effects	242:276	the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp	242:362	The aim of this study was to examine the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp.
24124012	4	66	dep	OFS	543:545	arg1	2					536:536	2	536:536	2	536:536	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	4	67	theme	n	588:588	arg1	rats/group					597:606	n = 9-10 rats/group	588:606	n = 9-10 rats/group	588:606	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	4	67	theme	n	588:588	arg1	OFS					563:565	(4) OFS	559:565	(4) OFS	559:565	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	11	68	theme	greater	1250:1256	arg1	magnitude					1258:1266	greater magnitude	1250:1266	greater magnitude	1250:1266	CONCLUSIONS The impact of prebiotic intake on body composition and gut microbiota was of greater magnitude than the probiotic BB-12.
24124012	12	69	theme	beneficial	1388:1397	arg1	role					1399:1402	the beneficial role	1384:1402	the beneficial role of each of these "biotic" agents in glycemic control	1384:1455	Despite this, an improvement in glucose AUC with both prebiotic or probiotic demonstrates the beneficial role of each of these "biotic" agents in glycemic control.
24124012	10	70	with	bifidobacteria	1065:1078	arg1	OFS					1113:1115	OFS	1113:1115	OFS that was not observed with probiotic alone	1113:1158	There was a marked increase in bifidobacteria and lactobacilli (P < 0.01) with OFS that was not observed with probiotic alone.
24124012	12	71	from	role	1399:1402	arg1	control					1449:1455	glycemic control	1440:1455	glycemic control	1440:1455	Despite this, an improvement in glucose AUC with both prebiotic or probiotic demonstrates the beneficial role of each of these "biotic" agents in glycemic control.
24124012	10	72	theme	marked	1046:1051	arg1	increase					1053:1060	a marked increase	1044:1060	a marked increase in bifidobacteria and lactobacilli (P < 0.01) with OFS that was not observed with probiotic alone	1044:1158	There was a marked increase in bifidobacteria and lactobacilli (P < 0.01) with OFS that was not observed with probiotic alone.
24124012	2	73	theme	subsp	358:362	arg1	effects					270:276	the individual and combined effects	242:276	the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp	242:362	The aim of this study was to examine the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp.
24124012	9	74	theme	probiotic	996:1004	arg1	GLP-2					1016:1020	probiotic increased GLP-2	996:1020	probiotic increased GLP-2 (P < 0.05)	996:1031	Portal GLP-1 was increased with OFS, whereas probiotic increased GLP-2 (P < 0.05).
24124012	9	74	theme	probiotic	996:1004	arg1	<					1025:1025	P < 0.05	1023:1030	P < 0.05	1023:1030	Portal GLP-1 was increased with OFS, whereas probiotic increased GLP-2 (P < 0.05).
24124012	6	75	theme	P	806:806	arg1	mass					800:803	fat mass	796:803	fat mass (P < 0.01)	796:814	RESULTS Prebiotic, but not probiotic reduced energy intake, weight gain, and fat mass (P < 0.01).
24124012	6	75	theme	P	806:806	arg1	<					808:808	P < 0.01	806:813	P < 0.01	806:813	RESULTS Prebiotic, but not probiotic reduced energy intake, weight gain, and fat mass (P < 0.01).
24124012	0	76	theme	gut	66:68	arg1	microbiota					70:79	gut microbiota	66:79	gut microbiota	66:79	Combined effects of oligofructose and Bifidobacterium animalis on gut microbiota and glycemia in obese rats.
24124012	4	77	theme	obese	472:476	arg1	rats					493:496	Adult male, diet-induced obese Sprague Dawley rats	447:496	Adult male, diet-induced obese Sprague Dawley rats	447:496	METHODS Adult male, diet-induced obese Sprague Dawley rats were randomized to: (1) Control (C); (2) 10% OFS; (3) BB-12; (4) OFS + BB-12 for 8 weeks (n = 9-10 rats/group).
24124012	8	78	theme	insulin	924:930	arg1	levels					932:937	insulin levels	924:937	insulin levels (P < 0.05)	924:948	Individually, OFS and BB-12 reduced insulin levels (P < 0.05).
24124012	8	78	theme	insulin	924:930	arg1	<					942:942	P < 0.05	940:947	P < 0.05	940:947	Individually, OFS and BB-12 reduced insulin levels (P < 0.05).
24124012	2	79	theme	animalis	349:356	arg1	subsp					358:362	the probiotic Bifidobacterium animalis subsp	319:362	the probiotic Bifidobacterium animalis subsp	319:362	The aim of this study was to examine the individual and combined effects of the prebiotic oligofructose (OFS) and the probiotic Bifidobacterium animalis subsp.
28485303	7	0	theme	growth	1189:1194	arg1	factor					1196:1201	vascular endothelial growth factor	1168:1201	vascular endothelial growth factor	1168:1201	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	4	1	theme	sheath	690:695	arg1	flow					697:700	as a sheath flow	685:700	as a sheath flow	685:700	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	4	1	theme	sheath	690:695	arg1	solution					620:627	the alginate solution	607:627	the alginate solution	607:627	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	4	1	theme	sheath	690:695	arg1	solution					641:648	a CaCl2 solution	633:648	a CaCl2 solution	633:648	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	1	2	theme	vascular	162:169	arg1	SDVGs					179:183	SDVGs	179:183	SDVGs	179:183	Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging.
28485303	1	2	theme	vascular	162:169	arg1	grafts					171:176	small diameter vascular grafts	147:176	small diameter vascular grafts (SDVGs)	147:184	Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging.
28485303	6	3	theme	engineered	998:1007	arg1	grafts					1009:1014	other tissue engineered grafts	985:1014	other tissue engineered grafts	985:1014	The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts.
28485303	0	4	theme	application	75:85	arg1	potential					87:95	the application potential	71:95	the application potential of small diameter vascular grafts	71:129	Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.
28485303	6	5	dep	engineered	998:1007	arg1	tissue					991:996	tissue	991:996	tissue	991:996	The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts.
28485303	1	6	theme	grafts	171:176	arg1	Fabrication					132:142	Fabrication	132:142	Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application	132:236	Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging.
28485303	0	7	theme	small	100:104	arg1	grafts					124:129	small diameter vascular grafts	100:129	small diameter vascular grafts	100:129	Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.
28485303	6	8	theme	porous	900:905	arg1	surfaces					907:914	somewhat rough and porous surfaces	881:914	somewhat rough and porous surfaces	881:914	The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts.
28485303	7	9	theme	stable	1108:1113	arg1	endothelialization					1115:1132	a stable endothelialization	1106:1132	a stable endothelialization	1106:1132	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	2	10	dep	production	285:294	arg1	the					281:283	the	281:283	the	281:283	In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original.
28485303	9	11	theme	future	1438:1443	arg1	alternative					1445:1455	a future alternative	1436:1455	a future alternative in SDVGs engineering	1436:1476	These encouraging results suggest great promise of the application of microfluidics as a future alternative in SDVGs engineering.
28485303	9	11	theme	future	1438:1443	arg1	promise					1389:1395	great promise	1383:1395	great promise of the application of microfluidics	1383:1431	These encouraging results suggest great promise of the application of microfluidics as a future alternative in SDVGs engineering.
28485303	6	12	theme	other	985:989	arg1	grafts					1009:1014	other tissue engineered grafts	985:1014	other tissue engineered grafts	985:1014	The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts.
28485303	7	13	theme	growth	1220:1225	arg1	factor					1227:1232	fibroblastic growth factor	1207:1232	fibroblastic growth factor	1207:1232	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	2	14	theme	SDVG	366:369	arg1	candidates					371:380	potential SDVG candidates	356:380	potential SDVG candidates	356:380	In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original.
28485303	4	15	theme	alginate	611:618	arg1	flow					697:700	as a sheath flow	685:700	as a sheath flow	685:700	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	4	15	theme	alginate	611:618	arg1	flow					676:679	a core flow	669:679	a core flow	669:679	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	4	15	theme	alginate	611:618	arg1	solution					620:627	the alginate solution	607:627	the alginate solution	607:627	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	4	15	theme	alginate	611:618	arg1	solution					641:648	a CaCl2 solution	633:648	a CaCl2 solution	633:648	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	0	16	theme	vascular	115:122	arg1	grafts					124:129	small diameter vascular grafts	100:129	small diameter vascular grafts	100:129	Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.
28485303	6	17	theme	rough	890:894	arg1	surfaces					907:914	somewhat rough and porous surfaces	881:914	somewhat rough and porous surfaces	881:914	The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts.
28485303	1	18	with	Fabrication	132:142	arg1	responses					203:211	appropriate responses	191:211	appropriate responses	191:211	Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging.
28485303	9	19	theme	SDVGs	1460:1464	arg1	engineering					1466:1476	SDVGs engineering	1460:1476	SDVGs engineering	1460:1476	These encouraging results suggest great promise of the application of microfluidics as a future alternative in SDVGs engineering.
28485303	2	20	theme	microfluidics	404:416	arg1	method					394:399	the method	390:399	the method of microfluidics	390:416	In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original.
28485303	0	21	theme	diameter	106:113	arg1	grafts					124:129	small diameter vascular grafts	100:129	small diameter vascular grafts	100:129	Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.
28485303	8	22	theme	in	1239:1240	arg1	implant					1247:1253	The in vivo implant	1235:1253	The in vivo implant into the mice abdomens	1235:1276	The in vivo implant into the mice abdomens indicated that cell composite microfibers caused a mild host reaction.
28485303	9	23	theme	microfluidics	1419:1431	arg1	application					1404:1414	the application	1400:1414	the application of microfluidics	1400:1431	These encouraging results suggest great promise of the application of microfluidics as a future alternative in SDVGs engineering.
28485303	3	24	theme	microfluidic	459:470	arg1	device					472:477	A simple glass microfluidic device	444:477	A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform	444:548	A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform was developed.
28485303	7	25	theme	factor	1196:1201	arg1	effects					1157:1163	the angiogenesis effects	1140:1163	the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor	1140:1232	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	9	26	theme	great	1383:1387	arg1	alternative					1445:1455	a future alternative	1436:1455	a future alternative in SDVGs engineering	1436:1476	These encouraging results suggest great promise of the application of microfluidics as a future alternative in SDVGs engineering.
28485303	9	26	theme	great	1383:1387	arg1	promise					1389:1395	great promise	1383:1395	great promise of the application of microfluidics	1383:1431	These encouraging results suggest great promise of the application of microfluidics as a future alternative in SDVGs engineering.
28485303	9	27	theme	encouraging	1355:1365	arg1	results					1367:1373	These encouraging results	1349:1373	These encouraging results	1349:1373	These encouraging results suggest great promise of the application of microfluidics as a future alternative in SDVGs engineering.
28485303	2	28	theme	solid	320:324	arg1	microfibers					341:351	solid alginate based microfibers	320:351	solid alginate based microfibers as potential SDVG candidates	320:380	In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original.
28485303	5	29	theme	glass	804:808	arg1	tubes					820:824	the glass capillary tubes	800:824	the glass capillary tubes	800:824	The diameters of the microfibers could be controlled by varying the flow rates and the glass capillary tubes diameters at their tips.
28485303	7	30	theme	vascular	1168:1175	arg1	factor					1196:1201	vascular endothelial growth factor	1168:1201	vascular endothelial growth factor	1168:1201	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	3	31	theme	simple	446:451	arg1	device					472:477	A simple glass microfluidic device	444:477	A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform	444:548	A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform was developed.
28485303	0	32	theme	composite	18:26	arg1	microfibers					37:47	cell composite alginate microfibers	13:47	cell composite alginate microfibers	13:47	Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.
28485303	8	33	theme	mild	1329:1332	arg1	reaction					1339:1346	a mild host reaction	1327:1346	a mild host reaction	1327:1346	The in vivo implant into the mice abdomens indicated that cell composite microfibers caused a mild host reaction.
28485303	3	34	with	device	472:477	arg1	channel					513:519	a 'L-shape' cylindrical-flow channel	484:519	a 'L-shape' cylindrical-flow channel	484:519	A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform was developed.
28485303	9	35	theme	application	1404:1414	arg1	alternative					1445:1455	a future alternative	1436:1455	a future alternative in SDVGs engineering	1436:1476	These encouraging results suggest great promise of the application of microfluidics as a future alternative in SDVGs engineering.
28485303	9	35	theme	application	1404:1414	arg1	promise					1389:1395	great promise	1383:1395	great promise of the application of microfluidics	1383:1431	These encouraging results suggest great promise of the application of microfluidics as a future alternative in SDVGs engineering.
28485303	1	36	theme	appropriate	191:201	arg1	responses					203:211	appropriate responses	191:211	appropriate responses	191:211	Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging.
28485303	0	37	theme	cell	13:16	arg1	microfibers					37:47	cell composite alginate microfibers	13:47	cell composite alginate microfibers	13:47	Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.
28485303	0	38	theme	grafts	124:129	arg1	potential					87:95	the application potential	71:95	the application potential of small diameter vascular grafts	71:129	Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.
28485303	2	39	theme	potential	356:364	arg1	candidates					371:380	potential SDVG candidates	356:380	potential SDVG candidates	356:380	In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original.
28485303	3	40	theme	microfluidic	528:539	arg1	platform					541:548	the microfluidic platform	524:548	the microfluidic platform	524:548	A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform was developed.
28485303	0	41	theme	microfibers	37:47	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.	0:130	Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.
28485303	4	42	theme	microfibers	581:591	arg1	gelation					569:576	The gelation	565:576	The gelation of microfibers	565:591	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	8	43	dep	in	1239:1240	arg1	vivo					1242:1245	vivo	1242:1245	vivo	1242:1245	The in vivo implant into the mice abdomens indicated that cell composite microfibers caused a mild host reaction.
28485303	6	44	theme	generated	855:863	arg1	microfibers					865:875	The generated microfibers	851:875	The generated microfibers	851:875	The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts.
28485303	8	45	theme	mice	1264:1267	arg1	abdomens					1269:1276	the mice abdomens	1260:1276	the mice abdomens	1260:1276	The in vivo implant into the mice abdomens indicated that cell composite microfibers caused a mild host reaction.
28485303	8	46	theme	composite	1298:1306	arg1	microfibers					1308:1318	cell composite microfibers	1293:1318	cell composite microfibers	1293:1318	The in vivo implant into the mice abdomens indicated that cell composite microfibers caused a mild host reaction.
28485303	0	47	theme	alginate	28:35	arg1	microfibers					37:47	cell composite alginate microfibers	13:47	cell composite alginate microfibers	13:47	Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.
28485303	5	48	theme	flow	785:788	arg1	rates					790:794	the flow rates	781:794	the flow rates	781:794	The diameters of the microfibers could be controlled by varying the flow rates and the glass capillary tubes diameters at their tips.
28485303	8	49	theme	host	1334:1337	arg1	reaction					1339:1346	a mild host reaction	1327:1346	a mild host reaction	1327:1346	The in vivo implant into the mice abdomens indicated that cell composite microfibers caused a mild host reaction.
28485303	5	50	theme	capillary	810:818	arg1	tubes					820:824	the glass capillary tubes	800:824	the glass capillary tubes	800:824	The diameters of the microfibers could be controlled by varying the flow rates and the glass capillary tubes diameters at their tips.
28485303	3	51	from	device	472:477	arg1	platform					541:548	the microfluidic platform	524:548	the microfluidic platform	524:548	A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform was developed.
28485303	1	52	theme	clinical	217:224	arg1	application					226:236	clinical application	217:236	clinical application	217:236	Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging.
28485303	7	53	theme	angiogenesis	1144:1155	arg1	effects					1157:1163	the angiogenesis effects	1140:1163	the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor	1140:1232	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	7	54	theme	factor	1227:1232	arg1	effects					1157:1163	the angiogenesis effects	1140:1163	the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor	1140:1232	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	3	55	theme	glass	453:457	arg1	device					472:477	A simple glass microfluidic device	444:477	A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform	444:548	A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform was developed.
28485303	7	56	theme	encapsulated	1021:1032	arg1	cells					1051:1055	The encapsulated mesenchymal stem cells	1017:1055	The encapsulated mesenchymal stem cells	1017:1055	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	3	57	theme	cylindrical-flow	496:511	arg1	channel					513:519	a 'L-shape' cylindrical-flow channel	484:519	a 'L-shape' cylindrical-flow channel	484:519	A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform was developed.
28485303	5	58	dep	varying	773:779	arg1	diameters					826:834	diameters	826:834	varying the flow rates and the glass capillary tubes diameters at their tips	773:848	The diameters of the microfibers could be controlled by varying the flow rates and the glass capillary tubes diameters at their tips.
28485303	6	59	theme	retention	930:938	arg1	strengths					940:948	their suture retention strengths	917:948	their suture retention strengths	917:948	The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts.
28485303	9	60	from	alternative	1445:1455	arg1	engineering					1466:1476	SDVGs engineering	1460:1476	SDVGs engineering	1460:1476	These encouraging results suggest great promise of the application of microfluidics as a future alternative in SDVGs engineering.
28485303	2	61	theme	microfibers	341:351	arg1	characterization					300:315	characterization	300:315	characterization	300:315	In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original.
28485303	2	61	theme	microfibers	341:351	arg1	production					285:294	production	285:294	production	285:294	In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original.
28485303	6	62	theme	suture	923:928	arg1	strengths					940:948	their suture retention strengths	917:948	their suture retention strengths	917:948	The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts.
28485303	7	63	theme	mesenchymal	1034:1044	arg1	cells					1051:1055	The encapsulated mesenchymal stem cells	1017:1055	The encapsulated mesenchymal stem cells	1017:1055	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	2	64	theme	present	267:273	arg1	work					275:278	the present work	263:278	the present work	263:278	In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original.
28485303	2	65	theme	based	335:339	arg1	microfibers					341:351	solid alginate based microfibers	320:351	solid alginate based microfibers as potential SDVG candidates	320:380	In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original.
28485303	5	66	theme	microfibers	738:748	arg1	diameters					721:729	The diameters	717:729	The diameters of the microfibers	717:748	The diameters of the microfibers could be controlled by varying the flow rates and the glass capillary tubes diameters at their tips.
28485303	7	67	theme	stem	1046:1049	arg1	cells					1051:1055	The encapsulated mesenchymal stem cells	1017:1055	The encapsulated mesenchymal stem cells	1017:1055	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	0	68	with	microfluidics	52:64	arg1	potential					87:95	the application potential	71:95	the application potential of small diameter vascular grafts	71:129	Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.
28485303	2	69	theme	alginate	326:333	arg1	microfibers					341:351	solid alginate based microfibers	320:351	solid alginate based microfibers as potential SDVG candidates	320:380	In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original.
28485303	7	70	theme	endothelial	1177:1187	arg1	factor					1196:1201	vascular endothelial growth factor	1168:1201	vascular endothelial growth factor	1168:1201	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	3	71	theme	L-shape	487:493	arg1	channel					513:519	a 'L-shape' cylindrical-flow channel	484:519	a 'L-shape' cylindrical-flow channel	484:519	A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform was developed.
28485303	8	72	theme	cell	1293:1296	arg1	microfibers					1308:1318	cell composite microfibers	1293:1318	cell composite microfibers	1293:1318	The in vivo implant into the mice abdomens indicated that cell composite microfibers caused a mild host reaction.
28485303	6	73	contain	had	877:879	arg2	surfaces					907:914	somewhat rough and porous surfaces	881:914	somewhat rough and porous surfaces	881:914	The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts.
28485303	6	73	contain	had	877:879	arg1	microfibers					865:875	The generated microfibers	851:875	The generated microfibers	851:875	The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts.
28485303	1	74	theme	small	147:151	arg1	SDVGs					179:183	SDVGs	179:183	SDVGs	179:183	Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging.
28485303	1	74	theme	small	147:151	arg1	grafts					171:176	small diameter vascular grafts	147:176	small diameter vascular grafts (SDVGs)	147:184	Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging.
28485303	4	75	theme	core	671:674	arg1	flow					676:679	a core flow	669:679	a core flow	669:679	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	4	75	theme	core	671:674	arg1	solution					620:627	the alginate solution	607:627	the alginate solution	607:627	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	4	75	theme	core	671:674	arg1	solution					641:648	a CaCl2 solution	633:648	a CaCl2 solution	633:648	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	7	76	theme	fibroblastic	1207:1218	arg1	factor					1227:1232	fibroblastic growth factor	1207:1232	fibroblastic growth factor	1207:1232	The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor.
28485303	6	77	theme	grafts	1009:1014	arg1	strength					973:980	the strength	969:980	the strength of other tissue engineered grafts	969:1014	The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts.
28485303	1	78	theme	diameter	153:160	arg1	SDVGs					179:183	SDVGs	179:183	SDVGs	179:183	Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging.
28485303	1	78	theme	diameter	153:160	arg1	grafts					171:176	small diameter vascular grafts	147:176	small diameter vascular grafts (SDVGs)	147:184	Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging.
28485303	4	79	theme	CaCl2	635:639	arg1	flow					697:700	as a sheath flow	685:700	as a sheath flow	685:700	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	4	79	theme	CaCl2	635:639	arg1	flow					676:679	a core flow	669:679	a core flow	669:679	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	4	79	theme	CaCl2	635:639	arg1	solution					620:627	the alginate solution	607:627	the alginate solution	607:627	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
28485303	4	79	theme	CaCl2	635:639	arg1	solution					641:648	a CaCl2 solution	633:648	a CaCl2 solution	633:648	The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively.
26385502	0	0	theme	efficient	65:73	arg1	adsorption					75:84	fast and efficient adsorption	56:84	fast and efficient adsorption of Cu(II) ions	56:99	A new porous magnetic chitosan modified by melamine for fast and efficient adsorption of Cu(II) ions.
26385502	1	1	theme	porous	108:113	arg1	chitosan					124:131	A new porous magnetic chitosan	102:131	A new porous magnetic chitosan modified by melamine (MA-CS/Fe3O4)	102:166	A new porous magnetic chitosan modified by melamine (MA-CS/Fe3O4) was synthesized.
26385502	5	2	theme	maximum	681:687	arg1	capacity					700:707	The maximum adsorption capacity	677:707	The maximum adsorption capacity for Cu(II)	677:718	The maximum adsorption capacity for Cu(II) was 2.58mmolg(-1) at the optimal experimental conditions, which were pH=5.5, t=25min, C0=5.0mmolL(-1).
26385502	1	3	theme	magnetic	115:122	arg1	chitosan					124:131	A new porous magnetic chitosan	102:131	A new porous magnetic chitosan modified by melamine (MA-CS/Fe3O4)	102:166	A new porous magnetic chitosan modified by melamine (MA-CS/Fe3O4) was synthesized.
26385502	7	4	theme	regeneration	982:993	arg1	cycles					995:1000	five regeneration cycles	977:1000	five regeneration cycles	977:1000	The adsorbent still exhibited high adsorption capacity after five regeneration cycles.
26385502	3	5	theme	pseudo-second-order	496:514	arg1	R>0.999					526:532	R>0.999	526:532	R>0.999	526:532	The results of adsorption kinetics showed the adsorption behavior could be better described by the pseudo-second-order equation (R>0.999).
26385502	3	5	theme	pseudo-second-order	496:514	arg1	equation					516:523	the pseudo-second-order equation	492:523	the pseudo-second-order equation (R>0.999)	492:533	The results of adsorption kinetics showed the adsorption behavior could be better described by the pseudo-second-order equation (R>0.999).
26385502	4	6	theme	0-1.0	670:674	arg1	range					661:665	the range	657:665	the range of 0-1.0	657:674	The adsorption isotherm was well fitted by the Langmuir equation (R>0.999), and the values of separation factors were in the range of 0-1.0.
26385502	2	7	theme	infrared	249:256	arg1	spectroscopy					263:274	infrared (IR) spectroscopy	249:274	infrared (IR) spectroscopy	249:274	The compositions and surface topographies were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis, thermogravimetric (TG) analysis and scanning electron microscope (SEM), respectively.
26385502	2	8	theme	X-ray	277:281	arg1	XRD					296:298	XRD	296:298	XRD	296:298	The compositions and surface topographies were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis, thermogravimetric (TG) analysis and scanning electron microscope (SEM), respectively.
26385502	2	8	theme	X-ray	277:281	arg1	diffraction					283:293	X-ray diffraction	277:293	X-ray diffraction (XRD) analysis	277:308	The compositions and surface topographies were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis, thermogravimetric (TG) analysis and scanning electron microscope (SEM), respectively.
26385502	4	9	theme	separation	630:639	arg1	factors					641:647	separation factors	630:647	separation factors	630:647	The adsorption isotherm was well fitted by the Langmuir equation (R>0.999), and the values of separation factors were in the range of 0-1.0.
26385502	0	10	theme	Cu	89:90	arg1	ions					96:99	Cu(II) ions	89:99	Cu(II) ions	89:99	A new porous magnetic chitosan modified by melamine for fast and efficient adsorption of Cu(II) ions.
26385502	3	11	dep	showed	432:437	arg1	described					479:487	described	479:487	showed the adsorption behavior could be better described by the pseudo-second-order equation (R>0.999)	432:533	The results of adsorption kinetics showed the adsorption behavior could be better described by the pseudo-second-order equation (R>0.999).
26385502	0	12	theme	new	2:4	arg1	chitosan					22:29	A new porous magnetic chitosan	0:29	A new porous magnetic chitosan	0:29	A new porous magnetic chitosan modified by melamine for fast and efficient adsorption of Cu(II) ions.
26385502	7	13	theme	adsorption	951:960	arg1	capacity					962:969	high adsorption capacity	946:969	high adsorption capacity	946:969	The adsorbent still exhibited high adsorption capacity after five regeneration cycles.
26385502	5	14	theme	optimal	745:751	arg1	conditions					766:775	the optimal experimental conditions	741:775	the optimal experimental conditions	741:775	The maximum adsorption capacity for Cu(II) was 2.58mmolg(-1) at the optimal experimental conditions, which were pH=5.5, t=25min, C0=5.0mmolL(-1).
26385502	5	14	theme	optimal	745:751	arg1	pH=5.5					789:794	pH=5.5	789:794	pH=5.5	789:794	The maximum adsorption capacity for Cu(II) was 2.58mmolg(-1) at the optimal experimental conditions, which were pH=5.5, t=25min, C0=5.0mmolL(-1).
26385502	4	15	theme	factors	641:647	arg1	values					620:625	the values	616:625	the values of separation factors	616:647	The adsorption isotherm was well fitted by the Langmuir equation (R>0.999), and the values of separation factors were in the range of 0-1.0.
26385502	5	16	theme	experimental	753:764	arg1	conditions					766:775	the optimal experimental conditions	741:775	the optimal experimental conditions	741:775	The maximum adsorption capacity for Cu(II) was 2.58mmolg(-1) at the optimal experimental conditions, which were pH=5.5, t=25min, C0=5.0mmolL(-1).
26385502	5	16	theme	experimental	753:764	arg1	pH=5.5					789:794	pH=5.5	789:794	pH=5.5	789:794	The maximum adsorption capacity for Cu(II) was 2.58mmolg(-1) at the optimal experimental conditions, which were pH=5.5, t=25min, C0=5.0mmolL(-1).
26385502	0	17	theme	magnetic	13:20	arg1	chitosan					22:29	A new porous magnetic chitosan	0:29	A new porous magnetic chitosan	0:29	A new porous magnetic chitosan modified by melamine for fast and efficient adsorption of Cu(II) ions.
26385502	5	18	dep	2.58mmolg	724:732	arg1	-1					734:735	-1	734:735	-1	734:735	The maximum adsorption capacity for Cu(II) was 2.58mmolg(-1) at the optimal experimental conditions, which were pH=5.5, t=25min, C0=5.0mmolL(-1).
26385502	0	19	theme	porous	6:11	arg1	chitosan					22:29	A new porous magnetic chitosan	0:29	A new porous magnetic chitosan	0:29	A new porous magnetic chitosan modified by melamine for fast and efficient adsorption of Cu(II) ions.
26385502	7	20	theme	high	946:949	arg1	capacity					962:969	high adsorption capacity	946:969	high adsorption capacity	946:969	The adsorbent still exhibited high adsorption capacity after five regeneration cycles.
26385502	3	21	theme	adsorption	443:452	arg1	behavior					454:461	the adsorption behavior	439:461	the adsorption behavior	439:461	The results of adsorption kinetics showed the adsorption behavior could be better described by the pseudo-second-order equation (R>0.999).
26385502	2	22	theme	electron	356:363	arg1	SEM					377:379	SEM	377:379	SEM	377:379	The compositions and surface topographies were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis, thermogravimetric (TG) analysis and scanning electron microscope (SEM), respectively.
26385502	2	22	theme	electron	356:363	arg1	microscope					365:374	scanning electron microscope	347:374	scanning electron microscope (SEM)	347:380	The compositions and surface topographies were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis, thermogravimetric (TG) analysis and scanning electron microscope (SEM), respectively.
26385502	5	23	theme	adsorption	689:698	arg1	capacity					700:707	The maximum adsorption capacity	677:707	The maximum adsorption capacity for Cu(II)	677:718	The maximum adsorption capacity for Cu(II) was 2.58mmolg(-1) at the optimal experimental conditions, which were pH=5.5, t=25min, C0=5.0mmolL(-1).
26385502	4	24	theme	Langmuir	583:590	arg1	R>0.999					602:608	R>0.999	602:608	R>0.999	602:608	The adsorption isotherm was well fitted by the Langmuir equation (R>0.999), and the values of separation factors were in the range of 0-1.0.
26385502	4	24	theme	Langmuir	583:590	arg1	equation					592:599	the Langmuir equation	579:599	the Langmuir equation (R>0.999)	579:609	The adsorption isotherm was well fitted by the Langmuir equation (R>0.999), and the values of separation factors were in the range of 0-1.0.
26385502	2	25	theme	scanning	347:354	arg1	SEM					377:379	SEM	377:379	SEM	377:379	The compositions and surface topographies were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis, thermogravimetric (TG) analysis and scanning electron microscope (SEM), respectively.
26385502	2	25	theme	scanning	347:354	arg1	microscope					365:374	scanning electron microscope	347:374	scanning electron microscope (SEM)	347:380	The compositions and surface topographies were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis, thermogravimetric (TG) analysis and scanning electron microscope (SEM), respectively.
26385502	8	26	theme	N	1071:1071	arg1	atoms					1073:1077	N atoms	1071:1077	N atoms	1071:1077	The adsorption mechanism was due to coordination between Cu(II) and N atoms.
26385502	0	27	theme	ions	96:99	arg1	adsorption					75:84	fast and efficient adsorption	56:84	fast and efficient adsorption of Cu(II) ions	56:99	A new porous magnetic chitosan modified by melamine for fast and efficient adsorption of Cu(II) ions.
26385502	6	28	theme	mass	900:903	arg1	transport					905:913	mass transport	900:913	chemical adsorption rather than mass transport	868:913	The rate-controlling step was supposed to be chemical adsorption rather than mass transport.
26385502	6	29	theme	rate-controlling	827:842	arg1	adsorption					877:886	chemical adsorption	868:886	chemical adsorption rather than mass transport	868:913	The rate-controlling step was supposed to be chemical adsorption rather than mass transport.
26385502	6	29	theme	rate-controlling	827:842	arg1	step					844:847	The rate-controlling step	823:847	The rate-controlling step	823:847	The rate-controlling step was supposed to be chemical adsorption rather than mass transport.
26385502	3	30	theme	kinetics	423:430	arg1	results					401:407	The results	397:407	The results of adsorption kinetics	397:430	The results of adsorption kinetics showed the adsorption behavior could be better described by the pseudo-second-order equation (R>0.999).
26385502	2	31	theme	IR	259:260	arg1	spectroscopy					263:274	infrared (IR) spectroscopy	249:274	infrared (IR) spectroscopy	249:274	The compositions and surface topographies were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis, thermogravimetric (TG) analysis and scanning electron microscope (SEM), respectively.
26385502	2	32	theme	diffraction	283:293	arg1	analysis					301:308	X-ray diffraction (XRD) analysis	277:308	X-ray diffraction (XRD) analysis	277:308	The compositions and surface topographies were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis, thermogravimetric (TG) analysis and scanning electron microscope (SEM), respectively.
26385502	3	33	theme	adsorption	412:421	arg1	kinetics					423:430	adsorption kinetics	412:430	adsorption kinetics	412:430	The results of adsorption kinetics showed the adsorption behavior could be better described by the pseudo-second-order equation (R>0.999).
26385502	4	34	theme	adsorption	540:549	arg1	isotherm					551:558	The adsorption isotherm	536:558	The adsorption isotherm	536:558	The adsorption isotherm was well fitted by the Langmuir equation (R>0.999), and the values of separation factors were in the range of 0-1.0.
26385502	0	35	theme	fast	56:59	arg1	adsorption					75:84	fast and efficient adsorption	56:84	fast and efficient adsorption of Cu(II) ions	56:99	A new porous magnetic chitosan modified by melamine for fast and efficient adsorption of Cu(II) ions.
26385502	2	36	theme	surface	206:212	arg1	topographies					214:225	surface topographies	206:225	surface topographies	206:225	The compositions and surface topographies were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis, thermogravimetric (TG) analysis and scanning electron microscope (SEM), respectively.
26385502	2	37	theme	thermogravimetric	311:327	arg1	analysis					334:341	thermogravimetric (TG) analysis	311:341	thermogravimetric (TG) analysis	311:341	The compositions and surface topographies were characterized by infrared (IR) spectroscopy, X-ray diffraction (XRD) analysis, thermogravimetric (TG) analysis and scanning electron microscope (SEM), respectively.
26385502	8	38	theme	adsorption	1007:1016	arg1	due					1032:1034	due	1032:1034	due	1032:1034	The adsorption mechanism was due to coordination between Cu(II) and N atoms.
26385502	8	38	theme	adsorption	1007:1016	arg1	mechanism					1018:1026	The adsorption mechanism	1003:1026	The adsorption mechanism	1003:1026	The adsorption mechanism was due to coordination between Cu(II) and N atoms.
26385502	1	39	theme	new	104:106	arg1	chitosan					124:131	A new porous magnetic chitosan	102:131	A new porous magnetic chitosan modified by melamine (MA-CS/Fe3O4)	102:166	A new porous magnetic chitosan modified by melamine (MA-CS/Fe3O4) was synthesized.
26385502	6	40	theme	chemical	868:875	arg1	adsorption					877:886	chemical adsorption	868:886	chemical adsorption rather than mass transport	868:913	The rate-controlling step was supposed to be chemical adsorption rather than mass transport.
26385502	6	40	theme	chemical	868:875	arg1	step					844:847	The rate-controlling step	823:847	The rate-controlling step	823:847	The rate-controlling step was supposed to be chemical adsorption rather than mass transport.
26812587	3	0	with	structures	524:533	arg1	from-and-at					571:581	from-and-at	571:581	from-and-at	571:581	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers are obtained without any directing agent.
26812587	3	0	with	structures	524:533	arg1	morphology					554:563	a flower-like morphology	540:563	a flower-like morphology grown from-and-at the surface of the cellulose fibers	540:617	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers are obtained without any directing agent.
26812587	4	1	from	°C.	842:844	arg1	pyrolysis					816:824	pyrolysis	816:824	pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials	816:975	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	0	2	theme	Efficient	78:86	arg1	Lithium					88:94	Efficient Lithium	78:94	Efficient Lithium	78:94	Synthesis of Titania@Carbon Nanocomposite from Urea-Impregnated Cellulose for Efficient Lithium and Sodium Batteries.
26812587	2	3	theme	anhydrous	366:374	arg1	conditions					376:385	anhydrous conditions	366:385	anhydrous conditions	366:385	By direct treatment with TiCl4 under anhydrous conditions, the urea impregnation of cellulose impacts both the TiO2 morphology and the carbon left by cellulose after pyrolysis.
26812587	1	4	theme	negative	275:282	arg1	nanocomposites					149:162	Nanostructured TiO2 and TiO2@C nanocomposites	118:162	Nanostructured TiO2 and TiO2@C nanocomposites	118:162	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	1	4	theme	negative	275:282	arg1	materials					294:302	negative electrode materials	275:302	negative electrode materials	275:302	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	0	5	theme	Sodium	100:105	arg1	Batteries					107:115	Sodium Batteries	100:115	Sodium Batteries	100:115	Synthesis of Titania@Carbon Nanocomposite from Urea-Impregnated Cellulose for Efficient Lithium and Sodium Batteries.
26812587	1	6	theme	electrode	284:292	arg1	nanocomposites					149:162	Nanostructured TiO2 and TiO2@C nanocomposites	118:162	Nanostructured TiO2 and TiO2@C nanocomposites	118:162	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	1	6	theme	electrode	284:292	arg1	materials					294:302	negative electrode materials	275:302	negative electrode materials	275:302	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	4	7	theme	electrode	957:965	arg1	materials					967:975	promising electrode materials	947:975	promising electrode materials	947:975	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	3	8	theme	Hierarchical	506:517	arg1	structures					524:533	Hierarchical TiO2 structures	506:533	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers	506:617	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers are obtained without any directing agent.
26812587	4	9	theme	Electrochemical	846:860	arg1	studies					862:868	Electrochemical studies	846:868	Electrochemical studies	846:868	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	4	10	theme	@	795:795	arg1	nanocomposites					798:811	TiO2@C nanocomposites	791:811	TiO2@C nanocomposites	791:811	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	2	11	theme	cellulose	413:421	arg1	impregnation					397:408	the urea impregnation	388:408	the urea impregnation of cellulose	388:421	By direct treatment with TiCl4 under anhydrous conditions, the urea impregnation of cellulose impacts both the TiO2 morphology and the carbon left by cellulose after pyrolysis.
26812587	4	12	theme	promising	947:955	arg1	materials					967:975	promising electrode materials	947:975	promising electrode materials	947:975	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	4	13	dep	can	900:902	arg1	de					905:906	de	905:906	de	905:906	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	3	14	dep	grown	565:569	arg1	from-and-at					571:581	from-and-at	571:581	from-and-at	571:581	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers are obtained without any directing agent.
26812587	3	14	dep	grown	565:569	arg1	morphology					554:563	a flower-like morphology	540:563	a flower-like morphology grown from-and-at the surface of the cellulose fibers	540:617	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers are obtained without any directing agent.
26812587	4	15	dep	°C.	842:844	arg1	demonstrate					870:880	demonstrate	870:880	demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials	870:975	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	3	16	theme	flower-like	542:552	arg1	from-and-at					571:581	from-and-at	571:581	from-and-at	571:581	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers are obtained without any directing agent.
26812587	3	16	theme	flower-like	542:552	arg1	morphology					554:563	a flower-like morphology	540:563	a flower-like morphology grown from-and-at the surface of the cellulose fibers	540:617	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers are obtained without any directing agent.
26812587	2	17	dep	carbon	464:469	arg1	left					471:474	left	471:474	left	471:474	By direct treatment with TiCl4 under anhydrous conditions, the urea impregnation of cellulose impacts both the TiO2 morphology and the carbon left by cellulose after pyrolysis.
26812587	4	18	from	°C	779:780	arg1	calcination					753:763	calcination	753:763	calcination in air at 600 °C	753:780	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	4	18	from	°C	779:780	arg1	air					768:770	air	768:770	air at 600 °C	768:780	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	2	19	theme	direct	332:337	arg1	treatment					339:347	direct treatment	332:347	direct treatment with TiCl4 under anhydrous conditions	332:385	By direct treatment with TiCl4 under anhydrous conditions, the urea impregnation of cellulose impacts both the TiO2 morphology and the carbon left by cellulose after pyrolysis.
26812587	3	20	theme	cellulose	602:610	arg1	fibers					612:617	the cellulose fibers	598:617	the cellulose fibers	598:617	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers are obtained without any directing agent.
26812587	1	21	theme	urea-impregnated	192:207	arg1	cellulose					209:217	urea-impregnated cellulose	192:217	urea-impregnated cellulose	192:217	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	0	22	theme	@	20:20	arg1	Nanocomposite					28:40	Titania@Carbon Nanocomposite	13:40	Titania@Carbon Nanocomposite	13:40	Synthesis of Titania@Carbon Nanocomposite from Urea-Impregnated Cellulose for Efficient Lithium and Sodium Batteries.
26812587	1	23	theme	Li	308:309	arg1	batteries					318:326	Li and Na batteries	308:326	Li and Na batteries	308:326	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	2	24	theme	TiO2	440:443	arg1	morphology					445:454	the TiO2 morphology	436:454	the TiO2 morphology	436:454	By direct treatment with TiCl4 under anhydrous conditions, the urea impregnation of cellulose impacts both the TiO2 morphology and the carbon left by cellulose after pyrolysis.
26812587	0	25	theme	Titania	13:19	arg1	Nanocomposite					28:40	Titania@Carbon Nanocomposite	13:40	Titania@Carbon Nanocomposite	13:40	Synthesis of Titania@Carbon Nanocomposite from Urea-Impregnated Cellulose for Efficient Lithium and Sodium Batteries.
26812587	3	26	theme	TiO2	519:522	arg1	structures					524:533	Hierarchical TiO2 structures	506:533	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers	506:617	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers are obtained without any directing agent.
26812587	4	27	theme	pure	732:735	arg1	flowers					742:748	pure TiO2 flowers	732:748	pure TiO2 flowers	732:748	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	0	28	theme	Nanocomposite	28:40	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Titania@Carbon Nanocomposite from Urea-Impregnated Cellulose for Efficient Lithium and Sodium Batteries.	0:116	Synthesis of Titania@Carbon Nanocomposite from Urea-Impregnated Cellulose for Efficient Lithium and Sodium Batteries.
26812587	1	29	theme	Na	315:316	arg1	batteries					318:326	Li and Na batteries	308:326	Li and Na batteries	308:326	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	2	30	with	treatment	339:347	arg1	TiCl4					354:358	TiCl4	354:358	TiCl4	354:358	By direct treatment with TiCl4 under anhydrous conditions, the urea impregnation of cellulose impacts both the TiO2 morphology and the carbon left by cellulose after pyrolysis.
26812587	2	31	theme	urea	392:395	arg1	impregnation					397:408	the urea impregnation	388:408	the urea impregnation of cellulose	388:421	By direct treatment with TiCl4 under anhydrous conditions, the urea impregnation of cellulose impacts both the TiO2 morphology and the carbon left by cellulose after pyrolysis.
26812587	0	32	theme	Carbon	21:26	arg1	Nanocomposite					28:40	Titania@Carbon Nanocomposite	13:40	Titania@Carbon Nanocomposite	13:40	Synthesis of Titania@Carbon Nanocomposite from Urea-Impregnated Cellulose for Efficient Lithium and Sodium Batteries.
26812587	4	33	theme	sodium	927:932	arg1	ions					934:937	insert lithium and sodium ions	908:937	ions	934:937	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	1	34	theme	simple	224:229	arg1	process					250:256	a simple reaction/diffusion process	222:256	a simple reaction/diffusion process	222:256	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	0	35	theme	Urea-Impregnated	47:62	arg1	Cellulose					64:72	Urea-Impregnated Cellulose	47:72	Urea-Impregnated Cellulose for Efficient Lithium and Sodium Batteries	47:115	Synthesis of Titania@Carbon Nanocomposite from Urea-Impregnated Cellulose for Efficient Lithium and Sodium Batteries.
26812587	0	36	from	Cellulose	64:72	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Titania@Carbon Nanocomposite from Urea-Impregnated Cellulose for Efficient Lithium and Sodium Batteries.	0:116	Synthesis of Titania@Carbon Nanocomposite from Urea-Impregnated Cellulose for Efficient Lithium and Sodium Batteries.
26812587	3	37	theme	fibers	612:617	arg1	surface					587:593	the surface	583:593	the surface of the cellulose fibers	583:617	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers are obtained without any directing agent.
26812587	1	38	theme	Nanostructured	118:131	arg1	TiO2					133:136	Nanostructured TiO2	118:136	Nanostructured TiO2	118:136	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	1	39	theme	TiO2	133:136	arg1	nanocomposites					149:162	Nanostructured TiO2 and TiO2@C nanocomposites	118:162	Nanostructured TiO2 and TiO2@C nanocomposites	118:162	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	1	39	theme	TiO2	133:136	arg1	materials					294:302	negative electrode materials	275:302	negative electrode materials	275:302	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	4	40	theme	insert	908:913	arg1	lithium					915:921	insert lithium and sodium ions	908:937	lithium	915:921	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	4	41	theme	TiO2	737:740	arg1	flowers					742:748	pure TiO2 flowers	732:748	pure TiO2 flowers	732:748	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	1	42	theme	reaction/diffusion	231:248	arg1	process					250:256	a simple reaction/diffusion process	222:256	a simple reaction/diffusion process	222:256	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	4	43	theme	TiO2	791:794	arg1	nanocomposites					798:811	TiO2@C nanocomposites	791:811	TiO2@C nanocomposites	791:811	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	4	44	theme	C	796:796	arg1	nanocomposites					798:811	TiO2@C nanocomposites	791:811	TiO2@C nanocomposites	791:811	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	1	45	theme	TiO2	142:145	arg1	C					147:147	TiO2@C	142:147	TiO2@C	142:147	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	4	46	from	calcination	753:763	arg1	°C					779:780	600 °C	775:780	600 °C	775:780	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	4	46	from	calcination	753:763	arg1	air					768:770	air	768:770	air at 600 °C	768:780	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	4	47	theme	TiO2/cellulose	675:688	arg1	composite					690:698	The resulting TiO2/cellulose composite	661:698	The resulting TiO2/cellulose composite	661:698	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	4	48	theme	resulting	665:673	arg1	composite					690:698	The resulting TiO2/cellulose composite	661:698	The resulting TiO2/cellulose composite	661:698	The resulting TiO2/cellulose composite is then transformed either into pure TiO2 flowers by calcination in air at 600 °C, or into TiO2@C nanocomposites by pyrolysis under Ar at 600 °C. Electrochemical studies demonstrate that both samples can (de)insert lithium and sodium ions and are promising electrode materials.
26812587	1	49	theme	@	146:146	arg1	C					147:147	TiO2@C	142:147	TiO2@C	142:147	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	3	50	theme	directing	644:652	arg1	agent					654:658	any directing agent	640:658	any directing agent	640:658	Hierarchical TiO2 structures with a flower-like morphology grown from-and-at the surface of the cellulose fibers are obtained without any directing agent.
26812587	1	51	theme	C	147:147	arg1	nanocomposites					149:162	Nanostructured TiO2 and TiO2@C nanocomposites	118:162	Nanostructured TiO2 and TiO2@C nanocomposites	118:162	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26812587	1	51	theme	C	147:147	arg1	materials					294:302	negative electrode materials	275:302	negative electrode materials	275:302	Nanostructured TiO2 and TiO2@C nanocomposites were prepared directly from urea-impregnated cellulose by a simple reaction/diffusion process and evaluated as negative electrode materials for Li and Na batteries.
26493462	3	0	dep	N-labeled	512:520	arg1	aureus					537:542	Staphylococcus aureus	522:542	Staphylococcus aureus	522:542	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	4	1	theme	acid	726:729	arg1	amounts					706:712	significant amounts	694:712	significant amounts of teichoic acid that contribute to cell-wall function	694:767	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	4	1	theme	acid	726:729	arg1	acid					726:729	teichoic acid	717:729	teichoic acid	717:729	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	6	2	theme	acid	926:929	arg1	d-Ala					931:935	Teichoic acid d-Ala	917:935	Teichoic acid d-Ala	917:935	Teichoic acid d-Ala is removed during cell-wall isolations and can only be detected in the context of whole cells.
26493462	7	3	theme	data	1125:1128	arg1	acquisition					1130:1140	2D data acquisition	1122:1140	2D data acquisition	1122:1140	The sequence presented here begins with fsREDOR and a chemical shift evolution period for 2D data acquisition, followed by DARR spin diffusion and then an additional fsREDOR period.
26493462	4	4	theme	significant	694:704	arg1	amounts					706:712	significant amounts	694:712	significant amounts of teichoic acid that contribute to cell-wall function	694:767	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	4	4	theme	significant	694:704	arg1	acid					726:729	teichoic acid	717:729	teichoic acid	717:729	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	7	5	dep	begins	1060:1065	arg1	followed					1143:1150	followed	1143:1150	followed by DARR spin diffusion and then an additional fsREDOR period	1143:1211	The sequence presented here begins with fsREDOR and a chemical shift evolution period for 2D data acquisition, followed by DARR spin diffusion and then an additional fsREDOR period.
26493462	1	6	theme	chemical	166:173	arg1	composition					175:185	chemical composition	166:185	chemical composition	166:185	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	1	7	theme	whole	252:256	arg1	cells					258:262	whole cells	252:262	whole cells	252:262	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	9	8	theme	interest	1476:1483	arg1	carbons					1518:1524	teichoic acid ribitol carbons	1496:1524	teichoic acid ribitol carbons	1496:1524	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	9	8	theme	interest	1476:1483	arg1	nitrogen					1464:1471	the selected amine nitrogen	1445:1471	the selected amine nitrogen of interest	1445:1483	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	8	9	theme	15	1346:1347	arg1	N					1349:1349	N	1349:1349	N	1349:1349	fsREDOR elements were used for (13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling and for (15)N dephasing to achieve selectivity in the nitrogens serving as dephasers.
26493462	4	10	theme	teichoic	717:724	arg1	acid					726:729	teichoic acid	717:729	teichoic acid	717:729	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	2	11	theme	whole	285:289	arg1	cells					291:295	whole cells	285:295	whole cells	285:295	Most NMR studies on whole cells have used selectively labeled samples.
26493462	1	12	theme	cells	258:262	arg1	context					241:247	the context	237:247	the context of whole cells	237:262	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	5	13	theme	ribitol	874:880	arg1	carbon					882:887	a ribitol carbon	872:887	a ribitol carbon	872:887	When incorporated into teichoic acid, d-alanine is present as an ester, connected via its carbonyl to a ribitol carbon, and thus has a free amine.
26493462	6	14	located	detected	992:999	arg1	context					1008:1014	the context	1004:1014	the context of whole cells	1004:1029	Teichoic acid d-Ala is removed during cell-wall isolations and can only be detected in the context of whole cells.
26493462	6	14	located	detected	992:999	arg2	d-Ala					931:935	Teichoic acid d-Ala	917:935	Teichoic acid d-Ala	917:935	Teichoic acid d-Ala is removed during cell-wall isolations and can only be detected in the context of whole cells.
26493462	8	15	from	selectivity	1372:1382	arg1	nitrogens					1391:1399	the nitrogens	1387:1399	the nitrogens serving as dephasers	1387:1420	fsREDOR elements were used for (13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling and for (15)N dephasing to achieve selectivity in the nitrogens serving as dephasers.
26493462	4	16	theme	structural	650:659	arg1	scaffold					661:668	the primary peptidoglycan structural scaffold	624:668	the primary peptidoglycan structural scaffold	624:668	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	4	17	theme	cell	671:674	arg1	walls					676:680	cell walls	671:680	cell walls	671:680	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	4	18	theme	peptidoglycan	636:648	arg1	scaffold					661:668	the primary peptidoglycan structural scaffold	624:668	the primary peptidoglycan structural scaffold	624:668	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	7	19	theme	DARR	1155:1158	arg1	diffusion					1165:1173	DARR spin diffusion	1155:1173	DARR spin diffusion	1155:1173	The sequence presented here begins with fsREDOR and a chemical shift evolution period for 2D data acquisition, followed by DARR spin diffusion and then an additional fsREDOR period.
26493462	2	20	theme	NMR	270:272	arg1	studies					274:280	Most NMR studies	265:280	Most NMR studies on whole cells	265:295	Most NMR studies on whole cells have used selectively labeled samples.
26493462	7	21	theme	evolution	1101:1109	arg1	period					1111:1116	a chemical shift evolution period	1084:1116	a chemical shift evolution period for 2D data acquisition	1084:1140	The sequence presented here begins with fsREDOR and a chemical shift evolution period for 2D data acquisition, followed by DARR spin diffusion and then an additional fsREDOR period.
26493462	8	22	theme	13	1246:1247	arg1	C					1249:1249	C	1249:1249	C	1249:1249	fsREDOR elements were used for (13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling and for (15)N dephasing to achieve selectivity in the nitrogens serving as dephasers.
26493462	9	23	theme	selected	1449:1456	arg1	carbons					1518:1524	teichoic acid ribitol carbons	1496:1524	teichoic acid ribitol carbons	1496:1524	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	9	23	theme	selected	1449:1456	arg1	nitrogen					1464:1471	the selected amine nitrogen	1445:1471	the selected amine nitrogen of interest	1445:1483	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	1	24	theme	Solid-state	86:96	arg1	method					137:142	a powerful and non-perturbative method	105:142	a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells	105:262	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	1	24	theme	Solid-state	86:96	arg1	NMR					98:100	Solid-state NMR	86:100	Solid-state NMR	86:100	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	5	25	contain	has	899:901	arg2	amine					910:914	a free amine	903:914	a free amine	903:914	When incorporated into teichoic acid, d-alanine is present as an ester, connected via its carbonyl to a ribitol carbon, and thus has a free amine.
26493462	5	25	contain	has	899:901	arg1	d-alanine					808:816	d-alanine	808:816	d-alanine	808:816	When incorporated into teichoic acid, d-alanine is present as an ester, connected via its carbonyl to a ribitol carbon, and thus has a free amine.
26493462	3	26	theme	spin	423:426	arg1	elements					438:445	spin diffusion elements	423:445	spin diffusion elements	423:445	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	3	27	theme	sequence	362:369	arg1	relay					371:375	an NMR sequence relay	355:375	an NMR sequence relay	355:375	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	3	28	theme	C-	501:502	arg1	cells					550:554	uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells	487:554	uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid	487:606	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	7	29	theme	additional	1187:1196	arg1	period					1206:1211	then an additional fsREDOR period	1179:1211	then an additional fsREDOR period	1179:1211	The sequence presented here begins with fsREDOR and a chemical shift evolution period for 2D data acquisition, followed by DARR spin diffusion and then an additional fsREDOR period.
26493462	3	30	theme	N-labeled	512:520	arg1	cells					550:554	uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells	487:554	uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid	487:606	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	8	31	theme	N	1349:1349	arg1	dephasing					1351:1359	(15)N dephasing	1345:1359	(15)N dephasing	1345:1359	fsREDOR elements were used for (13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling and for (15)N dephasing to achieve selectivity in the nitrogens serving as dephasers.
26493462	0	32	theme	Frequency-selective	0:18	arg1	REDOR					20:24	REDOR	20:24	REDOR	20:24	Frequency-selective REDOR and spin-diffusion relays in uniformly labeled whole cells.
26493462	9	33	theme	ribitol	1510:1516	arg1	carbons					1518:1524	teichoic acid ribitol carbons	1496:1524	teichoic acid ribitol carbons	1496:1524	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	9	33	theme	ribitol	1510:1516	arg1	nitrogen					1464:1471	the selected amine nitrogen	1445:1471	the selected amine nitrogen of interest	1445:1483	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	8	34	theme	C	1249:1249	arg1	observation					1251:1261	(13)C observation	1245:1261	(13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling	1245:1335	fsREDOR elements were used for (13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling and for (15)N dephasing to achieve selectivity in the nitrogens serving as dephasers.
26493462	4	35	dep	scaffold	661:668	arg1	addition					612:619	addition	612:619	addition	612:619	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	5	36	theme	free	905:908	arg1	amine					910:914	a free amine	903:914	a free amine	903:914	When incorporated into teichoic acid, d-alanine is present as an ester, connected via its carbonyl to a ribitol carbon, and thus has a free amine.
26493462	9	37	theme	acid	1505:1508	arg1	carbons					1518:1524	teichoic acid ribitol carbons	1496:1524	teichoic acid ribitol carbons	1496:1524	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	9	37	theme	acid	1505:1508	arg1	nitrogen					1464:1471	the selected amine nitrogen	1445:1471	the selected amine nitrogen of interest	1445:1483	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	10	38	theme	amide	1643:1647	arg1	nitrogens					1649:1657	amide nitrogens	1643:1657	amide nitrogens	1643:1657	In addition, its carbonyl is not significantly dephased by amide nitrogens, consistent with the expected microenvironment around teichoic acid.
26493462	1	39	theme	bacterial	207:215	arg1	walls					222:226	bacterial cell walls	207:226	bacterial cell walls	207:226	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	3	40	theme	NMR	358:360	arg1	relay					371:375	an NMR sequence relay	355:375	an NMR sequence relay	355:375	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	10	41	theme	expected	1680:1687	arg1	microenvironment					1689:1704	the expected microenvironment	1676:1704	the expected microenvironment around teichoic acid	1676:1725	In addition, its carbonyl is not significantly dephased by amide nitrogens, consistent with the expected microenvironment around teichoic acid.
26493462	3	42	theme	teichoic	594:601	arg1	acid					603:606	teichoic acid	594:606	teichoic acid	594:606	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	1	43	theme	powerful	107:114	arg1	method					137:142	a powerful and non-perturbative method	105:142	a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells	105:262	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	1	43	theme	powerful	107:114	arg1	NMR					98:100	Solid-state NMR	86:100	Solid-state NMR	86:100	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	1	44	theme	cell	217:220	arg1	walls					222:226	bacterial cell walls	207:226	bacterial cell walls	207:226	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	4	45	contain	contain	686:692	arg2	acid					726:729	teichoic acid	717:729	teichoic acid	717:729	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	4	45	contain	contain	686:692	arg2	amounts					706:712	significant amounts	694:712	significant amounts of teichoic acid that contribute to cell-wall function	694:767	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	4	45	contain	contain	686:692	arg1	walls					676:680	cell walls	671:680	cell walls	671:680	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	10	46	with	consistent	1660:1669	arg1	microenvironment					1689:1704	the expected microenvironment	1676:1704	the expected microenvironment around teichoic acid	1676:1725	In addition, its carbonyl is not significantly dephased by amide nitrogens, consistent with the expected microenvironment around teichoic acid.
26493462	3	47	theme	acid	603:606	arg1	d-alanine					581:589	the d-alanine	577:589	the d-alanine of teichoic acid	577:606	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	9	48	theme	teichoic	1496:1503	arg1	carbons					1518:1524	teichoic acid ribitol carbons	1496:1524	teichoic acid ribitol carbons	1496:1524	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	9	48	theme	teichoic	1496:1503	arg1	nitrogen					1464:1471	the selected amine nitrogen	1445:1471	the selected amine nitrogen of interest	1445:1483	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	2	49	from	studies	274:280	arg1	cells					291:295	whole cells	285:295	whole cells	285:295	Most NMR studies on whole cells have used selectively labeled samples.
26493462	3	50	theme	diffusion	428:436	arg1	elements					438:445	spin diffusion elements	423:445	spin diffusion elements	423:445	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	6	51	theme	Teichoic	917:924	arg1	d-Ala					931:935	Teichoic acid d-Ala	917:935	Teichoic acid d-Ala	917:935	Teichoic acid d-Ala is removed during cell-wall isolations and can only be detected in the context of whole cells.
26493462	8	52	theme	fsREDOR	1214:1220	arg1	elements					1222:1229	fsREDOR elements	1214:1229	fsREDOR elements	1214:1229	fsREDOR elements were used for (13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling and for (15)N dephasing to achieve selectivity in the nitrogens serving as dephasers.
26493462	6	53	theme	cell-wall	955:963	arg1	isolations					965:974	cell-wall isolations	955:974	cell-wall isolations	955:974	Teichoic acid d-Ala is removed during cell-wall isolations and can only be detected in the context of whole cells.
26493462	0	54	theme	labeled	65:71	arg1	cells					79:83	uniformly labeled whole cells	55:83	uniformly labeled whole cells	55:83	Frequency-selective REDOR and spin-diffusion relays in uniformly labeled whole cells.
26493462	9	55	theme	methyl	1539:1544	arg1	carbon					1552:1557	the methyl group carbon	1535:1557	the methyl group carbon associated with alanine	1535:1581	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	8	56	from	couplings	1303:1311	arg1	complications					1272:1284	complications	1272:1284	complications from (13)C-(13)C couplings	1272:1311	fsREDOR elements were used for (13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling and for (15)N dephasing to achieve selectivity in the nitrogens serving as dephasers.
26493462	6	57	theme	whole	1019:1023	arg1	cells					1025:1029	whole cells	1019:1029	whole cells	1019:1029	Teichoic acid d-Ala is removed during cell-wall isolations and can only be detected in the context of whole cells.
26493462	3	58	theme	amine	465:469	arg1	contribution					471:482	a unique amine contribution	456:482	a unique amine contribution	456:482	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	3	59	theme	frequency-selective	383:401	arg1	fsREDOR					410:416	fsREDOR	410:416	fsREDOR	410:416	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	3	59	theme	frequency-selective	383:401	arg1	REDOR					403:407	frequency-selective REDOR	383:407	frequency-selective REDOR (fsREDOR)	383:417	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	9	60	theme	group	1546:1550	arg1	carbon					1552:1557	the methyl group carbon	1535:1557	the methyl group carbon associated with alanine	1535:1581	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	8	61	theme	uniform	1320:1326	arg1	labeling					1328:1335	uniform labeling	1320:1335	uniform labeling	1320:1335	fsREDOR elements were used for (13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling and for (15)N dephasing to achieve selectivity in the nitrogens serving as dephasers.
26493462	3	62	theme	whole	544:548	arg1	cells					550:554	uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells	487:554	uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid	487:606	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	1	63	theme	non-perturbative	120:135	arg1	method					137:142	a powerful and non-perturbative method	105:142	a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells	105:262	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	1	63	theme	non-perturbative	120:135	arg1	NMR					98:100	Solid-state NMR	86:100	Solid-state NMR	86:100	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	7	64	theme	spin	1160:1163	arg1	diffusion					1165:1173	DARR spin diffusion	1155:1173	DARR spin diffusion	1155:1173	The sequence presented here begins with fsREDOR and a chemical shift evolution period for 2D data acquisition, followed by DARR spin diffusion and then an additional fsREDOR period.
26493462	10	65	theme	teichoic	1713:1720	arg1	acid					1722:1725	teichoic acid	1713:1725	teichoic acid	1713:1725	In addition, its carbonyl is not significantly dephased by amide nitrogens, consistent with the expected microenvironment around teichoic acid.
26493462	8	66	theme	C-	1295:1296	arg1	couplings					1303:1311	(13)C-(13)C couplings	1291:1311	(13)C-(13)C couplings	1291:1311	fsREDOR elements were used for (13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling and for (15)N dephasing to achieve selectivity in the nitrogens serving as dephasers.
26493462	2	67	theme	labeled	319:325	arg1	samples					327:333	selectively labeled samples	307:333	selectively labeled samples	307:333	Most NMR studies on whole cells have used selectively labeled samples.
26493462	6	68	theme	cells	1025:1029	arg1	context					1008:1014	the context	1004:1014	the context of whole cells	1004:1029	Teichoic acid d-Ala is removed during cell-wall isolations and can only be detected in the context of whole cells.
26493462	2	69	theme	Most	265:268	arg1	studies					274:280	Most NMR studies	265:280	Most NMR studies on whole cells	265:295	Most NMR studies on whole cells have used selectively labeled samples.
26493462	8	70	theme	C	1301:1301	arg1	couplings					1303:1311	(13)C-(13)C couplings	1291:1311	(13)C-(13)C couplings	1291:1311	fsREDOR elements were used for (13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling and for (15)N dephasing to achieve selectivity in the nitrogens serving as dephasers.
26493462	8	71	used	used	1236:1239	arg2	elements					1222:1229	fsREDOR elements	1214:1229	fsREDOR elements	1214:1229	fsREDOR elements were used for (13)C observation to avoid complications from (13)C-(13)C couplings due to uniform labeling and for (15)N dephasing to achieve selectivity in the nitrogens serving as dephasers.
26493462	1	72	from	architecture	191:202	arg1	walls					222:226	bacterial cell walls	207:226	bacterial cell walls	207:226	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	3	73	theme	unique	458:463	arg1	contribution					471:482	a unique amine contribution	456:482	a unique amine contribution	456:482	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	7	74	theme	fsREDOR	1198:1204	arg1	period					1206:1211	then an additional fsREDOR period	1179:1211	then an additional fsREDOR period	1179:1211	The sequence presented here begins with fsREDOR and a chemical shift evolution period for 2D data acquisition, followed by DARR spin diffusion and then an additional fsREDOR period.
26493462	9	75	theme	amine	1458:1462	arg1	carbons					1518:1524	teichoic acid ribitol carbons	1496:1524	teichoic acid ribitol carbons	1496:1524	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	9	75	theme	amine	1458:1462	arg1	nitrogen					1464:1471	the selected amine nitrogen	1445:1471	the selected amine nitrogen of interest	1445:1483	The results show that the selected amine nitrogen of interest is near to teichoic acid ribitol carbons and also the methyl group carbon associated with alanine.
26493462	5	76	theme	teichoic	793:800	arg1	acid					802:805	teichoic acid	793:805	teichoic acid	793:805	When incorporated into teichoic acid, d-alanine is present as an ester, connected via its carbonyl to a ribitol carbon, and thus has a free amine.
26493462	4	77	theme	primary	628:634	arg1	scaffold					661:668	the primary peptidoglycan structural scaffold	624:668	the primary peptidoglycan structural scaffold	624:668	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	2	78	used	used	302:305	arg2	cells					291:295	whole cells	285:295	whole cells	285:295	Most NMR studies on whole cells have used selectively labeled samples.
26493462	2	78	used	used	302:305	arg2	studies					274:280	Most NMR studies	265:280	Most NMR studies on whole cells	265:295	Most NMR studies on whole cells have used selectively labeled samples.
26493462	0	79	theme	whole	73:77	arg1	cells					79:83	uniformly labeled whole cells	55:83	uniformly labeled whole cells	55:83	Frequency-selective REDOR and spin-diffusion relays in uniformly labeled whole cells.
26493462	7	80	theme	shift	1095:1099	arg1	period					1111:1116	a chemical shift evolution period	1084:1116	a chemical shift evolution period for 2D data acquisition	1084:1140	The sequence presented here begins with fsREDOR and a chemical shift evolution period for 2D data acquisition, followed by DARR spin diffusion and then an additional fsREDOR period.
26493462	4	81	theme	cell-wall	750:758	arg1	function					760:767	cell-wall function	750:767	cell-wall function	750:767	In addition to the primary peptidoglycan structural scaffold, cell walls can contain significant amounts of teichoic acid that contribute to cell-wall function.
26493462	1	82	from	composition	175:185	arg1	walls					222:226	bacterial cell walls	207:226	bacterial cell walls	207:226	Solid-state NMR is a powerful and non-perturbative method to measure and define chemical composition and architecture in bacterial cell walls, even in the context of whole cells.
26493462	3	83	theme	13	498:499	arg1	C-					501:502	C-	501:502	C-	501:502	Here, we introduce an NMR sequence relay using frequency-selective REDOR (fsREDOR) and spin diffusion elements to probe a unique amine contribution in uniformly (13)C- and (15)N-labeled Staphylococcus aureus whole cells that we attribute to the d-alanine of teichoic acid.
26493462	7	84	theme	chemical	1086:1093	arg1	period					1111:1116	a chemical shift evolution period	1084:1116	a chemical shift evolution period for 2D data acquisition	1084:1140	The sequence presented here begins with fsREDOR and a chemical shift evolution period for 2D data acquisition, followed by DARR spin diffusion and then an additional fsREDOR period.
29133098	7	0	theme	oscillatory	1331:1341	arg1	shearing					1343:1350	oscillatory shearing	1331:1350	oscillatory shearing at 25°C	1331:1358	Modified samples presented better dissolution in water and less strong gels, with good stability during oscillatory shearing at 25°C.
29133098	1	1	theme	structural	143:152	arg1	modifications					170:182	the physical, structural and rheological modifications	129:182	the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin	129:243	The present study aimed to investigate the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin.
29133098	4	2	theme	uronic	734:739	arg1	%					760:760	up to 28.3%	750:760	up to 28.3%	750:760	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	4	2	theme	uronic	734:739	arg1	content					741:747	the acid uronic content	725:747	the acid uronic content (up to 28.3%)	725:761	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	4	3	dep	28.3	756:759	arg1	to					753:754	to	753:754	to	753:754	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	0	4	theme	rheologial	65:74	arg1	implications					76:87	Structural, physical and rheologial implications	40:87	Structural, physical and rheologial implications	40:87	Chemical modification of citrus pectin: Structural, physical and rheologial implications.
29133098	3	5	theme	citrus	651:656	arg1	MCP					667:669	MCP	667:669	MCP	667:669	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	5	theme	citrus	651:656	arg1	pectins					658:664	both native and modified citrus pectins	626:664	both native and modified citrus pectins (MCP)	626:670	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	5	6	theme	typical	997:1003	arg1	rhamnose					987:994	rhamnose	987:994	rhamnose	987:994	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	6	theme	typical	997:1003	arg1	amounts					919:925	higher amounts	912:925	higher amounts	912:925	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	6	theme	typical	997:1003	arg1	monosaccharides					938:952	neutral monosaccharides	930:952	neutral monosaccharides	930:952	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	6	theme	typical	997:1003	arg1	galactose					962:970	galactose	962:970	galactose	962:970	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	6	theme	typical	997:1003	arg1	arabinose					973:981	arabinose	973:981	arabinose	973:981	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	4	7	theme	chemical	695:702	arg1	modification					704:715	The chemical modification	691:715	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%)	691:791	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	4	8	theme	esterification	844:857	arg1	degree					834:839	the degree	830:839	the degree of esterification of native pectins	830:875	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	3	9	theme	pectins	658:664	arg1	homogeneity					568:578	homogeneity	568:578	homogeneity	568:578	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	9	theme	pectins	658:664	arg1	composition					538:548	monosaccharide composition	523:548	monosaccharide composition	523:548	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	9	theme	pectins	658:664	arg1	behavior					614:621	rheological behavior	602:621	rheological behavior of both native and modified citrus pectins (MCP)	602:670	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	9	theme	pectins	658:664	arg1	Mw					487:488	Mw	487:488	Mw	487:488	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	9	theme	pectins	658:664	arg1	morphology					581:590	morphology	581:590	morphology (SEM)	581:596	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	9	theme	pectins	658:664	arg1	SEM					593:595	SEM	593:595	SEM	593:595	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	9	theme	pectins	658:664	arg1	spectra					559:565	13C NMR spectra	551:565	13C NMR spectra	551:565	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	9	theme	pectins	658:664	arg1	degree					492:497	degree	492:497	degree of esterification (DE)	492:520	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	9	theme	pectins	658:664	arg1	mass					481:484	The molar mass	471:484	The molar mass (Mw)	471:489	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	10	theme	esterification	502:515	arg1	homogeneity					568:578	homogeneity	568:578	homogeneity	568:578	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	10	theme	esterification	502:515	arg1	composition					538:548	monosaccharide composition	523:548	monosaccharide composition	523:548	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	10	theme	esterification	502:515	arg1	behavior					614:621	rheological behavior	602:621	rheological behavior of both native and modified citrus pectins (MCP)	602:670	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	10	theme	esterification	502:515	arg1	Mw					487:488	Mw	487:488	Mw	487:488	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	10	theme	esterification	502:515	arg1	morphology					581:590	morphology	581:590	morphology (SEM)	581:596	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	10	theme	esterification	502:515	arg1	SEM					593:595	SEM	593:595	SEM	593:595	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	10	theme	esterification	502:515	arg1	spectra					559:565	13C NMR spectra	551:565	13C NMR spectra	551:565	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	10	theme	esterification	502:515	arg1	degree					492:497	degree	492:497	degree of esterification (DE)	492:520	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	10	theme	esterification	502:515	arg1	mass					481:484	The molar mass	471:484	The molar mass (Mw)	471:489	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	2	11	theme	different	294:302	arg1	degree					304:309	different degree	294:309	different degree of esterification	294:327	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	3	12	theme	13C	551:553	arg1	spectra					559:565	13C NMR spectra	551:565	13C NMR spectra	551:565	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	2	13	with	pectins	432:438	arg1	properties					459:468	special properties	451:468	special properties	451:468	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	3	14	theme	monosaccharide	523:536	arg1	composition					538:548	monosaccharide composition	523:548	monosaccharide composition	523:548	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	7	15	theme	good	1309:1312	arg1	stability					1314:1322	good stability	1309:1322	good stability during oscillatory shearing at 25°C	1309:1358	Modified samples presented better dissolution in water and less strong gels, with good stability during oscillatory shearing at 25°C.
29133098	4	16	theme	native	862:867	arg1	pectins					869:875	native pectins	862:875	native pectins	862:875	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	3	17	gly	homogeneity	568:578	arg1	DE					518:519	DE	518:519	DE	518:519	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	17	gly	homogeneity	568:578	arg1	MCP					667:669	MCP	667:669	MCP	667:669	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	17	gly	homogeneity	568:578	arg1	esterification					502:515	esterification	502:515	esterification (DE)	502:520	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	17	gly	homogeneity	568:578	arg1	pectins					658:664	both native and modified citrus pectins	626:664	both native and modified citrus pectins (MCP)	626:670	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	5	18	theme	Modified	878:885	arg1	pectins					894:900	Modified citrus pectins	878:900	Modified citrus pectins	878:900	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	1	19	theme	rheological	158:168	arg1	modifications					170:182	the physical, structural and rheological modifications	129:182	the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin	129:243	The present study aimed to investigate the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin.
29133098	4	20	dep	29.98	785:789	arg1	to					782:783	to	782:783	to	782:783	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	5	21	theme	Ramnogalacturonana-I	1012:1031	arg1	region					1040:1045	the Ramnogalacturonana-I (RG-I) region	1008:1045	the Ramnogalacturonana-I (RG-I) region	1008:1045	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	0	22	theme	Chemical	0:7	arg1	modification					9:20	Chemical modification	0:20	Chemical modification of citrus pectin	0:37	Chemical modification of citrus pectin: Structural, physical and rheologial implications.
29133098	7	23	from	25°C	1355:1358	arg1	shearing					1343:1350	oscillatory shearing	1331:1350	oscillatory shearing at 25°C	1331:1358	Modified samples presented better dissolution in water and less strong gels, with good stability during oscillatory shearing at 25°C.
29133098	6	24	theme	MCP	1167:1169	arg1	samples					1171:1177	the MCP samples	1163:1177	the MCP samples	1163:1177	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	24	theme	MCP	1167:1169	arg1	viscous					1189:1195	viscous	1189:1195	viscous	1189:1195	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	24	theme	MCP	1167:1169	arg1	compared					1198:1205	compared	1198:1205	compared to the native ones	1198:1224	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	0	25	dep	implications	76:87	arg1	modification					9:20	Chemical modification	0:20	Chemical modification of citrus pectin	0:37	Chemical modification of citrus pectin: Structural, physical and rheologial implications.
29133098	2	26	theme	special	451:457	arg1	properties					459:468	special properties	451:468	special properties	451:468	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	2	27	theme	esterification	314:327	arg1	degree					304:309	different degree	294:309	different degree of esterification	294:327	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	5	28	theme	region	1040:1045	arg1	typical					997:1003	typical	997:1003	typical	997:1003	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	29	theme	citrus	887:892	arg1	pectins					894:900	Modified citrus pectins	878:900	Modified citrus pectins	878:900	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	0	30	theme	citrus	25:30	arg1	pectin					32:37	citrus pectin	25:37	citrus pectin	25:37	Chemical modification of citrus pectin: Structural, physical and rheologial implications.
29133098	7	31	theme	better	1254:1259	arg1	dissolution					1261:1271	better dissolution	1254:1271	better dissolution	1254:1271	Modified samples presented better dissolution in water and less strong gels, with good stability during oscillatory shearing at 25°C.
29133098	1	32	theme	present	94:100	arg1	study					102:106	The present study	90:106	The present study	90:106	The present study aimed to investigate the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin.
29133098	3	33	theme	molar	475:479	arg1	Mw					487:488	Mw	487:488	Mw	487:488	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	33	theme	molar	475:479	arg1	mass					481:484	The molar mass	471:484	The molar mass (Mw)	471:489	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	34	theme	NMR	555:557	arg1	spectra					559:565	13C NMR spectra	551:565	13C NMR spectra	551:565	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	8	35	theme	citrus	1474:1479	arg1	pectins					1481:1487	citrus pectins	1474:1487	citrus pectins	1474:1487	This study aims to better understand the implications that chemical modifications may impose on the structure of citrus pectins.
29133098	2	36	theme	hydrolytic	386:395	arg1	process					397:403	sequential alkali and acidic hydrolytic process	357:403	process	397:403	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	6	37	theme	modified	1096:1103	arg1	viscous					1189:1195	viscous	1189:1195	viscous	1189:1195	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	37	theme	modified	1096:1103	arg1	pectins					1112:1118	the native and modified citrus pectins	1081:1118	the native and modified citrus pectins presented pseudoplastic behavior	1081:1151	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	37	theme	modified	1096:1103	arg1	compared					1198:1205	compared	1198:1205	compared to the native ones	1198:1224	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	38	theme	Rheological	1048:1058	arg1	tests					1060:1064	Rheological tests	1048:1064	Rheological tests	1048:1064	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	5	39	theme	monosaccharides	938:952	arg1	rhamnose					987:994	rhamnose	987:994	rhamnose	987:994	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	39	theme	monosaccharides	938:952	arg1	amounts					919:925	higher amounts	912:925	higher amounts	912:925	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	39	theme	monosaccharides	938:952	arg1	monosaccharides					938:952	neutral monosaccharides	930:952	neutral monosaccharides	930:952	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	39	theme	monosaccharides	938:952	arg1	galactose					962:970	galactose	962:970	galactose	962:970	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	39	theme	monosaccharides	938:952	arg1	arabinose					973:981	arabinose	973:981	arabinose	973:981	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	1	40	theme	chemical	198:205	arg1	process					220:226	the chemical modification process	194:226	the chemical modification process of citrus pectin	194:243	The present study aimed to investigate the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin.
29133098	7	41	theme	strong	1291:1296	arg1	gels					1298:1301	less strong gels	1286:1301	less strong gels	1286:1301	Modified samples presented better dissolution in water and less strong gels, with good stability during oscillatory shearing at 25°C.
29133098	7	42	theme	Modified	1227:1234	arg1	samples					1236:1242	Modified samples	1227:1242	Modified samples	1227:1242	Modified samples presented better dissolution in water and less strong gels, with good stability during oscillatory shearing at 25°C.
29133098	1	43	theme	modification	207:218	arg1	process					220:226	the chemical modification process	194:226	the chemical modification process of citrus pectin	194:243	The present study aimed to investigate the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin.
29133098	2	44	theme	modified	416:423	arg1	pectins					432:438	modified citrus pectins	416:438	modified citrus pectins (MCP) with special properties	416:468	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	2	44	theme	modified	416:423	arg1	MCP					441:443	MCP	441:443	MCP	441:443	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	0	45	theme	pectin	32:37	arg1	modification					9:20	Chemical modification	0:20	Chemical modification of citrus pectin	0:37	Chemical modification of citrus pectin: Structural, physical and rheologial implications.
29133098	5	46	theme	neutral	930:936	arg1	rhamnose					987:994	rhamnose	987:994	rhamnose	987:994	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	46	theme	neutral	930:936	arg1	arabinose					973:981	arabinose	973:981	arabinose	973:981	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	46	theme	neutral	930:936	arg1	monosaccharides					938:952	neutral monosaccharides	930:952	neutral monosaccharides	930:952	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	46	theme	neutral	930:936	arg1	galactose					962:970	galactose	962:970	galactose	962:970	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	2	47	theme	citrus	425:430	arg1	pectins					432:438	modified citrus pectins	416:438	modified citrus pectins (MCP) with special properties	416:468	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	2	47	theme	citrus	425:430	arg1	MCP					441:443	MCP	441:443	MCP	441:443	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	6	48	dep	viscous	1189:1195	arg1	samples					1171:1177	the MCP samples	1163:1177	the MCP samples	1163:1177	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	48	dep	viscous	1189:1195	arg1	viscous					1189:1195	viscous	1189:1195	viscous	1189:1195	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	48	dep	viscous	1189:1195	arg1	pectins					1112:1118	the native and modified citrus pectins	1081:1118	the native and modified citrus pectins presented pseudoplastic behavior	1081:1151	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	48	dep	viscous	1189:1195	arg1	compared					1198:1205	compared	1198:1205	compared to the native ones	1198:1224	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	3	49	theme	rheological	602:612	arg1	behavior					614:621	rheological behavior	602:621	rheological behavior of both native and modified citrus pectins (MCP)	602:670	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	4	50	theme	molar	767:771	arg1	%					790:790	up to 29.98%	779:790	up to 29.98%	779:790	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	4	50	theme	molar	767:771	arg1	mass					773:776	molar mass	767:776	molar mass (up to 29.98%)	767:791	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	2	51	theme	sequential	357:366	arg1	alkali					368:373	sequential alkali and acidic hydrolytic process	357:403	alkali	368:373	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	0	52	theme	Structural	40:49	arg1	implications					76:87	Structural, physical and rheologial implications	40:87	Structural, physical and rheologial implications	40:87	Chemical modification of citrus pectin: Structural, physical and rheologial implications.
29133098	6	53	theme	native	1214:1219	arg1	ones					1221:1224	the native ones	1210:1224	the native ones	1210:1224	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	7	54	attach	presented	1244:1252	arg2	samples					1236:1242	Modified samples	1227:1242	Modified samples	1227:1242	Modified samples presented better dissolution in water and less strong gels, with good stability during oscillatory shearing at 25°C.
29133098	7	54	attach	presented	1244:1252	arg1	water					1276:1280	water	1276:1280	water	1276:1280	Modified samples presented better dissolution in water and less strong gels, with good stability during oscillatory shearing at 25°C.
29133098	7	54	attach	presented	1244:1252	arg1	gels					1298:1301	less strong gels	1286:1301	less strong gels	1286:1301	Modified samples presented better dissolution in water and less strong gels, with good stability during oscillatory shearing at 25°C.
29133098	4	55	theme	pectins	869:875	arg1	esterification					844:857	esterification	844:857	esterification of native pectins	844:875	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	4	56	theme	little	810:815	arg1	influence					817:825	little influence	810:825	little influence	810:825	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	3	57	theme	modified	642:649	arg1	MCP					667:669	MCP	667:669	MCP	667:669	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	57	theme	modified	642:649	arg1	pectins					658:664	both native and modified citrus pectins	626:664	both native and modified citrus pectins (MCP)	626:670	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	2	58	theme	commercial	263:272	arg1	pectins					281:287	three commercial citrus pectins	257:287	three commercial citrus pectins with different degree of esterification	257:327	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	2	59	mod	modified	345:352	arg3	process					397:403	sequential alkali and acidic hydrolytic process	357:403	process	397:403	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	2	59	mod	modified	345:352	arg3	alkali					368:373	sequential alkali and acidic hydrolytic process	357:403	alkali	368:373	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	2	59	mod	modified	345:352	arg1	pectins					281:287	three commercial citrus pectins	257:287	three commercial citrus pectins with different degree of esterification	257:327	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	2	60	with	pectins	281:287	arg1	degree					304:309	different degree	294:309	different degree of esterification	294:327	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	2	61	theme	citrus	274:279	arg1	pectins					281:287	three commercial citrus pectins	257:287	three commercial citrus pectins with different degree of esterification	257:327	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	6	62	theme	pseudoplastic	1130:1142	arg1	behavior					1144:1151	pseudoplastic behavior	1130:1151	pseudoplastic behavior	1130:1151	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	4	63	theme	acid	729:732	arg1	%					760:760	up to 28.3%	750:760	up to 28.3%	750:760	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	4	63	theme	acid	729:732	arg1	content					741:747	the acid uronic content	725:747	the acid uronic content (up to 28.3%)	725:761	The chemical modification reduced the acid uronic content (up to 28.3%) and molar mass (up to 29.98%), however, showed little influence on the degree of esterification of native pectins.
29133098	1	64	theme	citrus	231:236	arg1	pectin					238:243	citrus pectin	231:243	citrus pectin	231:243	The present study aimed to investigate the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin.
29133098	8	65	theme	chemical	1420:1427	arg1	modifications					1429:1441	chemical modifications	1420:1441	chemical modifications	1420:1441	This study aims to better understand the implications that chemical modifications may impose on the structure of citrus pectins.
29133098	3	66	theme	native	631:636	arg1	MCP					667:669	MCP	667:669	MCP	667:669	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	3	66	theme	native	631:636	arg1	pectins					658:664	both native and modified citrus pectins	626:664	both native and modified citrus pectins (MCP)	626:670	The molar mass (Mw), degree of esterification (DE), monosaccharide composition, 13C NMR spectra, homogeneity, morphology (SEM) and rheological behavior of both native and modified citrus pectins (MCP) were investigated.
29133098	5	67	theme	higher	912:917	arg1	rhamnose					987:994	rhamnose	987:994	rhamnose	987:994	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	67	theme	higher	912:917	arg1	amounts					919:925	higher amounts	912:925	higher amounts	912:925	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	67	theme	higher	912:917	arg1	monosaccharides					938:952	neutral monosaccharides	930:952	neutral monosaccharides	930:952	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	67	theme	higher	912:917	arg1	galactose					962:970	galactose	962:970	galactose	962:970	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	5	67	theme	higher	912:917	arg1	arabinose					973:981	arabinose	973:981	arabinose	973:981	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	1	68	theme	physical	133:140	arg1	modifications					170:182	the physical, structural and rheological modifications	129:182	the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin	129:243	The present study aimed to investigate the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin.
29133098	6	69	theme	native	1085:1090	arg1	viscous					1189:1195	viscous	1189:1195	viscous	1189:1195	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	69	theme	native	1085:1090	arg1	pectins					1112:1118	the native and modified citrus pectins	1081:1118	the native and modified citrus pectins presented pseudoplastic behavior	1081:1151	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	69	theme	native	1085:1090	arg1	compared					1198:1205	compared	1198:1205	compared to the native ones	1198:1224	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	1	70	theme	pectin	238:243	arg1	process					220:226	the chemical modification process	194:226	the chemical modification process of citrus pectin	194:243	The present study aimed to investigate the physical, structural and rheological modifications caused by the chemical modification process of citrus pectin.
29133098	0	71	theme	physical	52:59	arg1	implications					76:87	Structural, physical and rheologial implications	40:87	Structural, physical and rheologial implications	40:87	Chemical modification of citrus pectin: Structural, physical and rheologial implications.
29133098	5	72	theme	RG-I	1034:1037	arg1	region					1040:1045	the Ramnogalacturonana-I (RG-I) region	1008:1045	the Ramnogalacturonana-I (RG-I) region	1008:1045	Modified citrus pectins presented higher amounts of neutral monosaccharides, mainly galactose, arabinose and rhamnose, typical of the Ramnogalacturonana-I (RG-I) region.
29133098	6	73	theme	citrus	1105:1110	arg1	viscous					1189:1195	viscous	1189:1195	viscous	1189:1195	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	73	theme	citrus	1105:1110	arg1	pectins					1112:1118	the native and modified citrus pectins	1081:1118	the native and modified citrus pectins presented pseudoplastic behavior	1081:1151	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	6	73	theme	citrus	1105:1110	arg1	compared					1198:1205	compared	1198:1205	compared to the native ones	1198:1224	Rheological tests indicated that the native and modified citrus pectins presented pseudoplastic behavior, however, the MCP samples were less viscous, compared to the native ones.
29133098	2	74	theme	acidic	379:384	arg1	process					397:403	sequential alkali and acidic hydrolytic process	357:403	process	397:403	Therefore, three commercial citrus pectins with different degree of esterification were chemically modified by sequential alkali and acidic hydrolytic process to produce modified citrus pectins (MCP) with special properties.
29133098	8	75	theme	pectins	1481:1487	arg1	structure					1461:1469	the structure	1457:1469	the structure of citrus pectins	1457:1487	This study aims to better understand the implications that chemical modifications may impose on the structure of citrus pectins.
24528765	0	0	theme	vulgaris	76:83	arg1	pectins					101:107	Silene vulgaris callus modified pectins	69:107	Silene vulgaris callus modified pectins	69:107	Swelling and morphology of calcium pectinate gel beads obtained from Silene vulgaris callus modified pectins.
24528765	1	1	dep	nutrients	300:308	arg1	concentration					327:339	concentration	327:339	concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)	327:402	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	1	1	dep	nutrients	300:308	arg1	sources					318:324	carbon sources	311:324	carbon sources	311:324	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	4	2	theme	auxin	775:779	arg1	absence					764:770	the absence	760:770	the absence of auxin	760:779	The swelling of dried CaPG beads in solutions with pH 2 and pH 4 was demonstrated to occur more slowly (within 4 or 24h) with increasing sucrose and calcium concentrations or in the absence of auxin.
24528765	8	3	theme	CaPG	1264:1267	arg1	beads					1269:1273	the CaPG beads	1260:1273	the CaPG beads	1260:1273	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	0	4	theme	Silene	69:74	arg1	pectins					101:107	Silene vulgaris callus modified pectins	69:107	Silene vulgaris callus modified pectins	69:107	Swelling and morphology of calcium pectinate gel beads obtained from Silene vulgaris callus modified pectins.
24528765	1	5	theme	carbon	311:316	arg1	concentration					327:339	concentration	327:339	concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)	327:402	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	1	5	theme	carbon	311:316	arg1	sources					318:324	carbon sources	311:324	carbon sources	311:324	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	6	6	theme	beads	929:933	arg1	morphology					906:915	The surface morphology	894:915	The surface morphology of the CaPG beads	894:933	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	1	7	theme	beads	228:232	arg1	properties					166:175	the swelling properties	153:175	the swelling properties	153:175	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	1	7	theme	beads	228:232	arg1	morphology					181:190	morphology	181:190	morphology	181:190	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	0	8	theme	modified	92:99	arg1	pectins					101:107	Silene vulgaris callus modified pectins	69:107	Silene vulgaris callus modified pectins	69:107	Swelling and morphology of calcium pectinate gel beads obtained from Silene vulgaris callus modified pectins.
24528765	8	9	theme	resultant	1325:1333	arg1	hydrogels					1345:1353	the resultant pectinate hydrogels	1321:1353	the resultant pectinate hydrogels in physiological environments	1321:1383	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	9	10	theme	2,4-D0	1425:1430	arg1	swelling					1405:1412	the swelling	1401:1412	the swelling of Ca 4.5, 2,4-D0, Suc30 and Suc100 CaPG beads	1401:1459	In particular, the swelling of Ca 4.5, 2,4-D0, Suc30 and Suc100 CaPG beads occurred more slowly.
24528765	9	11	theme	beads	1455:1459	arg1	swelling					1405:1412	the swelling	1401:1412	the swelling of Ca 4.5, 2,4-D0, Suc30 and Suc100 CaPG beads	1401:1459	In particular, the swelling of Ca 4.5, 2,4-D0, Suc30 and Suc100 CaPG beads occurred more slowly.
24528765	9	12	theme	Ca	1417:1418	arg1	swelling					1405:1412	the swelling	1401:1412	the swelling of Ca 4.5, 2,4-D0, Suc30 and Suc100 CaPG beads	1401:1459	In particular, the swelling of Ca 4.5, 2,4-D0, Suc30 and Suc100 CaPG beads occurred more slowly.
24528765	0	13	theme	callus	85:90	arg1	pectins					101:107	Silene vulgaris callus modified pectins	69:107	Silene vulgaris callus modified pectins	69:107	Swelling and morphology of calcium pectinate gel beads obtained from Silene vulgaris callus modified pectins.
24528765	8	14	theme	involving	1154:1162	arg1	strategy					1145:1152	An applied strategy involving	1134:1162	An applied strategy involving changing the composition and concentration of media components	1134:1225	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	5	15	dep	media	827:831	arg1	pH					834:835	pH 2	834:837	pH 2	834:837	All beads swelled less when placed in acidic media (pH 2 and pH 4) and swelled most extensively in NaCl (pH 6).
24528765	5	15	dep	media	827:831	arg1	pH					843:844	pH 4	843:846	pH 4	843:846	All beads swelled less when placed in acidic media (pH 2 and pH 4) and swelled most extensively in NaCl (pH 6).
24528765	5	15	dep	media	827:831	arg1	media					827:831	acidic media	820:831	acidic media (pH 2 and pH 4)	820:847	All beads swelled less when placed in acidic media (pH 2 and pH 4) and swelled most extensively in NaCl (pH 6).
24528765	7	16	theme	dried	1067:1071	arg1	beads					1078:1082	dried CaPG beads	1067:1082	dried CaPG beads	1067:1082	The slow swelling of dried CaPG beads was apparently related to their grooved surfaces.
24528765	6	17	theme	CaPG	924:927	arg1	beads					929:933	the CaPG beads	920:933	the CaPG beads	920:933	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	4	18	theme	beads	609:613	arg1	swelling					586:593	The swelling	582:593	The swelling of dried CaPG beads in solutions with pH 2 and pH 4	582:645	The swelling of dried CaPG beads in solutions with pH 2 and pH 4 was demonstrated to occur more slowly (within 4 or 24h) with increasing sucrose and calcium concentrations or in the absence of auxin.
24528765	8	19	from	hydrogels	1345:1353	arg1	environments					1372:1383	physiological environments	1358:1383	physiological environments	1358:1383	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	8	20	from	environments	1372:1383	arg1	resistance					1307:1316	the acid and water resistance	1288:1316	resistance	1307:1316	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	8	21	theme	acid	1292:1295	arg1	resistance					1307:1316	the acid and water resistance	1288:1316	resistance	1307:1316	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	1	22	theme	swelling	157:164	arg1	properties					166:175	the swelling properties	153:175	the swelling properties	153:175	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	1	23	theme	sucrose	344:350	arg1	concentration					327:339	concentration	327:339	concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)	327:402	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	1	23	theme	sucrose	344:350	arg1	sources					318:324	carbon sources	311:324	carbon sources	311:324	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	8	24	theme	components	1216:1225	arg1	concentration					1193:1205	concentration	1193:1205	concentration	1193:1205	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	8	24	theme	components	1216:1225	arg1	composition					1177:1187	composition	1177:1187	composition	1177:1187	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	3	25	theme	beads	521:525	arg1	volume					501:506	volume	501:506	volume	501:506	The mean diameter, total surface area and volume of the dried beads varied depending on the plant cell culture conditions.
24528765	3	25	theme	beads	521:525	arg1	area					492:495	total surface area	478:495	total surface area	478:495	The mean diameter, total surface area and volume of the dried beads varied depending on the plant cell culture conditions.
24528765	3	25	theme	beads	521:525	arg1	diameter					468:475	The mean diameter	459:475	The mean diameter	459:475	The mean diameter, total surface area and volume of the dried beads varied depending on the plant cell culture conditions.
24528765	8	26	theme	hydrogels	1345:1353	arg1	resistance					1307:1316	the acid and water resistance	1288:1316	resistance	1307:1316	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	9	27	theme	Suc100	1443:1448	arg1	beads					1455:1459	Suc100 CaPG beads	1443:1459	Suc100 CaPG beads	1443:1459	In particular, the swelling of Ca 4.5, 2,4-D0, Suc30 and Suc100 CaPG beads occurred more slowly.
24528765	2	28	theme	Gelled	406:411	arg1	spheres					413:419	Gelled spheres	406:419	Gelled spheres	406:419	Gelled spheres were prepared by ionotropic gelation.
24528765	8	29	dep	composition	1177:1187	arg1	the					1173:1175	the	1173:1175	the	1173:1175	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	8	30	theme	beads	1269:1273	arg1	behavior					1248:1255	the swelling behavior	1235:1255	the swelling behavior of the CaPG beads	1235:1273	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	6	31	theme	cell	1020:1023	arg1	medium					1033:1038	the plant cell culture medium	1010:1038	the plant cell culture medium used	1010:1043	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	9	32	theme	CaPG	1450:1453	arg1	beads					1455:1459	Suc100 CaPG beads	1443:1459	Suc100 CaPG beads	1443:1459	In particular, the swelling of Ca 4.5, 2,4-D0, Suc30 and Suc100 CaPG beads occurred more slowly.
24528765	6	33	theme	sugars	981:986	arg1	presence					969:976	the presence	965:976	the presence of sugars, calcium and auxin in the plant cell culture medium used	965:1043	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	6	34	theme	surface	898:904	arg1	morphology					906:915	The surface morphology	894:915	The surface morphology of the CaPG beads	894:933	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	1	35	theme	campion	255:261	arg1	callus					263:268	campion callus	255:268	campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D))	255:403	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	4	36	with	solutions	618:626	arg1	pH					633:634	pH 2	633:636	pH 2	633:636	The swelling of dried CaPG beads in solutions with pH 2 and pH 4 was demonstrated to occur more slowly (within 4 or 24h) with increasing sucrose and calcium concentrations or in the absence of auxin.
24528765	4	36	with	solutions	618:626	arg1	pH					642:643	pH 4	642:645	pH 4	642:645	The swelling of dried CaPG beads in solutions with pH 2 and pH 4 was demonstrated to occur more slowly (within 4 or 24h) with increasing sucrose and calcium concentrations or in the absence of auxin.
24528765	3	37	theme	dried	515:519	arg1	beads					521:525	the dried beads	511:525	the dried beads	511:525	The mean diameter, total surface area and volume of the dried beads varied depending on the plant cell culture conditions.
24528765	4	38	from	swelling	586:593	arg1	solutions					618:626	solutions	618:626	solutions with pH 2 and pH 4	618:645	The swelling of dried CaPG beads in solutions with pH 2 and pH 4 was demonstrated to occur more slowly (within 4 or 24h) with increasing sucrose and calcium concentrations or in the absence of auxin.
24528765	3	39	theme	total	478:482	arg1	area					492:495	total surface area	478:495	total surface area	478:495	The mean diameter, total surface area and volume of the dried beads varied depending on the plant cell culture conditions.
24528765	2	40	theme	ionotropic	438:447	arg1	gelation					449:456	ionotropic gelation	438:456	ionotropic gelation	438:456	Gelled spheres were prepared by ionotropic gelation.
24528765	1	41	theme	callus	263:268	arg1	pectins					244:250	pectins	244:250	pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D))	244:403	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	1	42	theme	calcium	353:359	arg1	concentration					327:339	concentration	327:339	concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)	327:402	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	1	42	theme	calcium	353:359	arg1	sources					318:324	carbon sources	311:324	carbon sources	311:324	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	6	43	theme	plant	1014:1018	arg1	medium					1033:1038	the plant cell culture medium	1010:1038	the plant cell culture medium used	1010:1043	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	4	44	theme	dried	598:602	arg1	beads					609:613	dried CaPG beads	598:613	dried CaPG beads	598:613	The swelling of dried CaPG beads in solutions with pH 2 and pH 4 was demonstrated to occur more slowly (within 4 or 24h) with increasing sucrose and calcium concentrations or in the absence of auxin.
24528765	0	45	theme	calcium	27:33	arg1	morphology					13:22	morphology	13:22	morphology	13:22	Swelling and morphology of calcium pectinate gel beads obtained from Silene vulgaris callus modified pectins.
24528765	0	45	theme	calcium	27:33	arg1	Swelling					0:7	Swelling	0:7	Swelling	0:7	Swelling and morphology of calcium pectinate gel beads obtained from Silene vulgaris callus modified pectins.
24528765	3	46	theme	plant	551:555	arg1	conditions					570:579	the plant cell culture conditions	547:579	the plant cell culture conditions	547:579	The mean diameter, total surface area and volume of the dried beads varied depending on the plant cell culture conditions.
24528765	8	47	theme	applied	1137:1143	arg1	strategy					1145:1152	An applied strategy involving	1134:1162	An applied strategy involving changing the composition and concentration of media components	1134:1225	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	1	48	theme	calcium	199:205	arg1	beads					228:232	the calcium pectinate gel (CaPG) beads	195:232	the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D))	195:403	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	8	49	theme	physiological	1358:1370	arg1	environments					1372:1383	physiological environments	1358:1383	physiological environments	1358:1383	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	8	50	from	resistance	1307:1316	arg1	environments					1372:1383	physiological environments	1358:1383	physiological environments	1358:1383	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	1	51	theme	2,4-dichlorophenoxyacetic	365:389	arg1	2,4-D					397:401	2,4-D	397:401	2,4-D	397:401	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	1	51	theme	2,4-dichlorophenoxyacetic	365:389	arg1	acid					391:394	2,4-dichlorophenoxyacetic acid	365:394	2,4-dichlorophenoxyacetic acid (2,4-D)	365:402	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	8	52	theme	swelling	1239:1246	arg1	behavior					1248:1255	the swelling behavior	1235:1255	the swelling behavior of the CaPG beads	1235:1273	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	4	53	dep	slowly	678:683	arg1	24h					698:700	24h	698:700	24h	698:700	The swelling of dried CaPG beads in solutions with pH 2 and pH 4 was demonstrated to occur more slowly (within 4 or 24h) with increasing sucrose and calcium concentrations or in the absence of auxin.
24528765	4	53	dep	slowly	678:683	arg1	4					693:693	4	693:693	4	693:693	The swelling of dried CaPG beads in solutions with pH 2 and pH 4 was demonstrated to occur more slowly (within 4 or 24h) with increasing sucrose and calcium concentrations or in the absence of auxin.
24528765	0	54	theme	gel	45:47	arg1	beads					49:53	gel beads	45:53	gel beads obtained from Silene vulgaris callus modified pectins	45:107	Swelling and morphology of calcium pectinate gel beads obtained from Silene vulgaris callus modified pectins.
24528765	1	55	theme	various	285:291	arg1	nutrients					300:308	various medium nutrients	285:308	various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D))	285:403	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	1	56	theme	acid	391:394	arg1	concentration					327:339	concentration	327:339	concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)	327:402	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	1	56	theme	acid	391:394	arg1	sources					318:324	carbon sources	311:324	carbon sources	311:324	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	4	57	theme	calcium	731:737	arg1	concentrations					739:752	sucrose and calcium concentrations	719:752	concentrations	739:752	The swelling of dried CaPG beads in solutions with pH 2 and pH 4 was demonstrated to occur more slowly (within 4 or 24h) with increasing sucrose and calcium concentrations or in the absence of auxin.
24528765	6	58	from	presence	969:976	arg1	medium					1033:1038	the plant cell culture medium	1010:1038	the plant cell culture medium used	1010:1043	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	3	59	theme	mean	463:466	arg1	diameter					468:475	The mean diameter	459:475	The mean diameter	459:475	The mean diameter, total surface area and volume of the dried beads varied depending on the plant cell culture conditions.
24528765	1	60	theme	medium	293:298	arg1	nutrients					300:308	various medium nutrients	285:308	various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D))	285:403	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	6	61	attach	presence	969:976	arg2	auxin					1001:1005	auxin	1001:1005	auxin	1001:1005	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	6	61	attach	presence	969:976	arg1	medium					1033:1038	the plant cell culture medium	1010:1038	the plant cell culture medium used	1010:1043	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	6	61	attach	presence	969:976	arg2	calcium					989:995	calcium	989:995	calcium	989:995	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	6	61	attach	presence	969:976	arg2	sugars					981:986	sugars	981:986	sugars	981:986	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	9	62	theme	Suc30	1433:1437	arg1	swelling					1405:1412	the swelling	1401:1412	the swelling of Ca 4.5, 2,4-D0, Suc30 and Suc100 CaPG beads	1401:1459	In particular, the swelling of Ca 4.5, 2,4-D0, Suc30 and Suc100 CaPG beads occurred more slowly.
24528765	4	63	theme	sucrose	719:725	arg1	concentrations					739:752	sucrose and calcium concentrations	719:752	concentrations	739:752	The swelling of dried CaPG beads in solutions with pH 2 and pH 4 was demonstrated to occur more slowly (within 4 or 24h) with increasing sucrose and calcium concentrations or in the absence of auxin.
24528765	5	64	theme	acidic	820:825	arg1	pH					834:835	pH 2	834:837	pH 2	834:837	All beads swelled less when placed in acidic media (pH 2 and pH 4) and swelled most extensively in NaCl (pH 6).
24528765	5	64	theme	acidic	820:825	arg1	pH					843:844	pH 4	843:846	pH 4	843:846	All beads swelled less when placed in acidic media (pH 2 and pH 4) and swelled most extensively in NaCl (pH 6).
24528765	5	64	theme	acidic	820:825	arg1	media					827:831	acidic media	820:831	acidic media (pH 2 and pH 4)	820:847	All beads swelled less when placed in acidic media (pH 2 and pH 4) and swelled most extensively in NaCl (pH 6).
24528765	8	65	theme	media	1210:1214	arg1	components					1216:1225	media components	1210:1225	media components	1210:1225	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	1	66	theme	pectinate	207:215	arg1	beads					228:232	the calcium pectinate gel (CaPG) beads	195:232	the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D))	195:403	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	6	67	theme	culture	1025:1031	arg1	medium					1033:1038	the plant cell culture medium	1010:1038	the plant cell culture medium used	1010:1043	The surface morphology of the CaPG beads was demonstrated to depend on the presence of sugars, calcium and auxin in the plant cell culture medium used.
24528765	7	68	theme	CaPG	1073:1076	arg1	beads					1078:1082	dried CaPG beads	1067:1082	dried CaPG beads	1067:1082	The slow swelling of dried CaPG beads was apparently related to their grooved surfaces.
24528765	1	69	theme	gel	217:219	arg1	beads					228:232	the calcium pectinate gel (CaPG) beads	195:232	the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D))	195:403	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	8	70	theme	pectinate	1335:1343	arg1	hydrogels					1345:1353	the resultant pectinate hydrogels	1321:1353	the resultant pectinate hydrogels in physiological environments	1321:1383	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	8	71	theme	water	1301:1305	arg1	resistance					1307:1316	the acid and water resistance	1288:1316	resistance	1307:1316	An applied strategy involving changing the composition and concentration of media components altered the swelling behavior of the CaPG beads and enhanced the acid and water resistance of the resultant pectinate hydrogels in physiological environments.
24528765	3	72	theme	cell	557:560	arg1	conditions					570:579	the plant cell culture conditions	547:579	the plant cell culture conditions	547:579	The mean diameter, total surface area and volume of the dried beads varied depending on the plant cell culture conditions.
24528765	7	73	theme	grooved	1116:1122	arg1	surfaces					1124:1131	their grooved surfaces	1110:1131	their grooved surfaces	1110:1131	The slow swelling of dried CaPG beads was apparently related to their grooved surfaces.
24528765	1	74	theme	research	126:133	arg1	aim					114:116	The aim	110:116	The aim of this research	110:133	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
24528765	4	75	theme	CaPG	604:607	arg1	beads					609:613	dried CaPG beads	598:613	dried CaPG beads	598:613	The swelling of dried CaPG beads in solutions with pH 2 and pH 4 was demonstrated to occur more slowly (within 4 or 24h) with increasing sucrose and calcium concentrations or in the absence of auxin.
24528765	7	76	theme	slow	1050:1053	arg1	swelling					1055:1062	The slow swelling	1046:1062	The slow swelling of dried CaPG beads	1046:1082	The slow swelling of dried CaPG beads was apparently related to their grooved surfaces.
24528765	7	76	theme	slow	1050:1053	arg1	related					1099:1105	related	1099:1105	related	1099:1105	The slow swelling of dried CaPG beads was apparently related to their grooved surfaces.
24528765	3	77	theme	culture	562:568	arg1	conditions					570:579	the plant cell culture conditions	547:579	the plant cell culture conditions	547:579	The mean diameter, total surface area and volume of the dried beads varied depending on the plant cell culture conditions.
24528765	7	78	theme	beads	1078:1082	arg1	swelling					1055:1062	The slow swelling	1046:1062	The slow swelling of dried CaPG beads	1046:1082	The slow swelling of dried CaPG beads was apparently related to their grooved surfaces.
24528765	7	78	theme	beads	1078:1082	arg1	related					1099:1105	related	1099:1105	related	1099:1105	The slow swelling of dried CaPG beads was apparently related to their grooved surfaces.
24528765	3	79	theme	surface	484:490	arg1	area					492:495	total surface area	478:495	total surface area	478:495	The mean diameter, total surface area and volume of the dried beads varied depending on the plant cell culture conditions.
24528765	1	80	theme	CaPG	222:225	arg1	beads					228:232	the calcium pectinate gel (CaPG) beads	195:232	the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D))	195:403	The aim of this research is to investigate the swelling properties and morphology of the calcium pectinate gel (CaPG) beads made from pectins of campion callus cultured using various medium nutrients (carbon sources, concentration of sucrose, calcium and 2,4-dichlorophenoxyacetic acid (2,4-D)).
25325958	1	0	theme	HS	297:298	arg1	pathway					313:319	the HS biosynthetic pathway	293:319	the HS biosynthetic pathway	293:319	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	2	1	theme	groups	672:677	arg1	positioning					649:659	positioning	649:659	positioning	649:659	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	2	1	theme	groups	672:677	arg1	number					638:643	number	638:643	number	638:643	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	0	2	theme	populations	69:79	arg1	characterization					26:41	characterization	26:41	characterization	26:41	Use of flow cytometry for characterization and fractionation of cell populations based on their expression of heparan sulfate epitopes.
25325958	0	2	theme	populations	69:79	arg1	fractionation					47:59	fractionation	47:59	fractionation	47:59	Use of flow cytometry for characterization and fractionation of cell populations based on their expression of heparan sulfate epitopes.
25325958	1	3	theme	biosynthetic	300:311	arg1	pathway					313:319	the HS biosynthetic pathway	293:319	the HS biosynthetic pathway	293:319	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	3	4	theme	changes	801:807	arg1	characterization					777:792	the rapid characterization	767:792	the rapid characterization of the changes in HS-specific antibody binding	767:839	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	2	5	theme	chain	487:491	arg1	composition					493:503	HS chain composition	484:503	HS chain composition	484:503	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	1	6	theme	pathway	313:319	arg1	components					279:288	one or more components	267:288	one or more components of the HS biosynthetic pathway	267:319	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	2	7	theme	antibodies	544:553	arg1	multitude					521:529	a multitude	519:529	a multitude of different antibodies	519:553	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	1	8	theme	cell/tissue	399:409	arg1	function					411:418	cell/tissue function	399:418	cell/tissue function	399:418	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	2	9	theme	HS	484:485	arg1	composition					493:503	HS chain composition	484:503	HS chain composition	484:503	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	2	10	theme	different	534:542	arg1	antibodies					544:553	different antibodies	534:553	different antibodies	534:553	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	1	11	theme	loss	247:250	arg1	result					237:242	a result	235:242	a result of loss or mutation of one or more components of the HS biosynthetic pathway	235:319	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	2	12	theme	subtle	592:597	arg1	differences					599:609	subtle differences	592:609	subtle differences in HS patterning	592:626	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	4	13	theme	cell	939:942	arg1	FACS					953:956	FACS	953:956	FACS	953:956	Additionally fluorescent-activated cell sorting (FACS) allows fractionation of cells based on their HS-epitope expression.
25325958	4	13	theme	cell	939:942	arg1	sorting					944:950	fluorescent-activated cell sorting	917:950	fluorescent-activated cell sorting (FACS)	917:957	Additionally fluorescent-activated cell sorting (FACS) allows fractionation of cells based on their HS-epitope expression.
25325958	2	14	theme	antibodies	440:449	arg1	use					425:427	The use	421:427	The use of anti-HS antibodies	421:449	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	3	15	theme	rapid	771:775	arg1	characterization					777:792	the rapid characterization	767:792	the rapid characterization of the changes in HS-specific antibody binding	767:839	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	3	16	theme	multiple	859:866	arg1	types					873:877	multiple cell types	859:877	multiple cell types	859:877	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	1	17	theme	broad	338:342	arg1	understanding					344:356	broad understanding	338:356	broad understanding of the effects these changes may have on cell/tissue function	338:418	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	3	18	theme	cell	868:871	arg1	types					873:877	multiple cell types	859:877	multiple cell types	859:877	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	1	19	theme	heparan	179:185	arg1	HS					196:197	HS	196:197	HS	196:197	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	1	19	theme	heparan	179:185	arg1	sulfate					187:193	heparan sulfate	179:193	heparan sulfate (HS) structure	179:208	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	3	20	theme	Flow	718:721	arg1	cytometry					723:731	Flow cytometry	718:731	Flow cytometry	718:731	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	3	20	theme	Flow	718:721	arg1	technique					747:755	a valuable technique	736:755	a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ	736:847	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	1	21	theme	sulfate	187:193	arg1	structure					200:208	heparan sulfate (HS) structure	179:208	heparan sulfate (HS) structure	179:208	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	0	22	theme	cytometry	12:20	arg1	Use					0:2	Use	0:2	Use of flow cytometry for characterization and fractionation of cell populations	0:79	Use of flow cytometry for characterization and fractionation of cell populations based on their expression of heparan sulfate epitopes.
25325958	1	23	theme	mutation	255:262	arg1	result					237:242	a result	235:242	a result of loss or mutation of one or more components of the HS biosynthetic pathway	235:319	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	0	24	theme	sulfate	118:124	arg1	epitopes					126:133	heparan sulfate epitopes	110:133	heparan sulfate epitopes	110:133	Use of flow cytometry for characterization and fractionation of cell populations based on their expression of heparan sulfate epitopes.
25325958	0	25	theme	flow	7:10	arg1	cytometry					12:20	flow cytometry	7:20	flow cytometry	7:20	Use of flow cytometry for characterization and fractionation of cell populations based on their expression of heparan sulfate epitopes.
25325958	4	26	theme	cells	983:987	arg1	fractionation					966:978	fractionation	966:978	fractionation of cells based on their HS-epitope expression	966:1024	Additionally fluorescent-activated cell sorting (FACS) allows fractionation of cells based on their HS-epitope expression.
25325958	0	27	theme	heparan	110:116	arg1	sulfate					118:124	heparan sulfate	110:124	heparan sulfate epitopes	110:133	Use of flow cytometry for characterization and fractionation of cell populations based on their expression of heparan sulfate epitopes.
25325958	3	28	from	binding	833:839	arg1	characterization					777:792	the rapid characterization	767:792	the rapid characterization of the changes in HS-specific antibody binding	767:839	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	2	29	theme	anti-HS	432:438	arg1	antibodies					440:449	anti-HS antibodies	432:449	anti-HS antibodies	432:449	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	3	30	theme	valuable	738:745	arg1	cytometry					723:731	Flow cytometry	718:731	Flow cytometry	718:731	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	3	30	theme	valuable	738:745	arg1	technique					747:755	a valuable technique	736:755	a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ	736:847	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	0	31	theme	epitopes	126:133	arg1	expression					96:105	their expression	90:105	their expression of heparan sulfate epitopes	90:133	Use of flow cytometry for characterization and fractionation of cell populations based on their expression of heparan sulfate epitopes.
25325958	2	32	theme	sulfate	664:670	arg1	groups					672:677	sulfate groups	664:677	sulfate groups influencing antibody binding affinity	664:715	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	4	33	theme	fluorescent-activated	917:937	arg1	FACS					953:956	FACS	953:956	FACS	953:956	Additionally fluorescent-activated cell sorting (FACS) allows fractionation of cells based on their HS-epitope expression.
25325958	4	33	theme	fluorescent-activated	917:937	arg1	sorting					944:950	fluorescent-activated cell sorting	917:950	fluorescent-activated cell sorting (FACS)	917:957	Additionally fluorescent-activated cell sorting (FACS) allows fractionation of cells based on their HS-epitope expression.
25325958	1	34	theme	effects	365:371	arg1	understanding					344:356	broad understanding	338:356	broad understanding of the effects these changes may have on cell/tissue function	338:418	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	3	35	from	changes	801:807	arg1	binding					833:839	HS-specific antibody binding	812:839	HS-specific antibody binding	812:839	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	1	36	theme	components	279:288	arg1	mutation					255:262	mutation	255:262	mutation	255:262	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	1	36	theme	components	279:288	arg1	loss					247:250	loss	247:250	loss	247:250	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	3	37	from	characterization	777:792	arg1	binding					833:839	HS-specific antibody binding	812:839	HS-specific antibody binding	812:839	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	2	38	from	differences	599:609	arg1	patterning					617:626	HS patterning	614:626	HS patterning	614:626	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	3	39	theme	antibody	824:831	arg1	binding					833:839	HS-specific antibody binding	812:839	HS-specific antibody binding	812:839	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	2	40	theme	HS	614:615	arg1	patterning					617:626	HS patterning	614:626	HS patterning	614:626	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	2	41	theme	binding	700:706	arg1	affinity					708:715	antibody binding affinity	691:715	antibody binding affinity	691:715	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	2	42	dep	number	638:643	arg1	the					634:636	the	634:636	the	634:636	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	1	43	dep	effects	365:371	arg1	have					391:394	have	391:394	may have on cell/tissue function	387:418	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25325958	0	44	theme	cell	64:67	arg1	populations					69:79	cell populations	64:79	cell populations	64:79	Use of flow cytometry for characterization and fractionation of cell populations based on their expression of heparan sulfate epitopes.
25325958	2	45	theme	antibody	691:698	arg1	affinity					708:715	antibody binding affinity	691:715	antibody binding affinity	691:715	The use of anti-HS antibodies provides an opportunity to study HS chain composition in situ, with a multitude of different antibodies having been generated that recognize subtle differences in HS patterning, with the number and positioning of sulfate groups influencing antibody binding affinity.
25325958	3	46	theme	HS-specific	812:822	arg1	binding					833:839	HS-specific antibody binding	812:839	HS-specific antibody binding	812:839	Flow cytometry is a valuable technique to enable the rapid characterization of the changes in HS-specific antibody binding in situ, allowing multiple cell types to be directly compared.
25325958	4	47	theme	HS-epitope	1004:1013	arg1	expression					1015:1024	their HS-epitope expression	998:1024	their HS-epitope expression	998:1024	Additionally fluorescent-activated cell sorting (FACS) allows fractionation of cells based on their HS-epitope expression.
25325958	1	48	from	alterations	164:174	arg1	structure					200:208	heparan sulfate (HS) structure	179:208	heparan sulfate (HS) structure	179:208	The ability to characterize alterations in heparan sulfate (HS) structure during development or as a result of loss or mutation of one or more components of the HS biosynthetic pathway is essential for broad understanding of the effects these changes may have on cell/tissue function.
25129795	2	0	theme	significant	376:386	arg1	drop					397:400	a significant pressure drop	374:400	a significant pressure drop during field applications	374:426	n-HAp powder utilized as a promising defluoridating material, but it causes a significant pressure drop during field applications.
25129795	4	1	theme	n-HApAlg	557:564	arg1	composite					566:574	synthesized n-HApAlg composite	545:574	synthesized n-HApAlg composite	545:574	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	1	2	theme	Alg	180:182	arg1	nano-hydroxyapatite					202:220	alginate (Alg) bioencapsulating nano-hydroxyapatite	170:220	alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely	170:235	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	1	2	theme	Alg	180:182	arg1	n-HAp					223:227	n-HAp	223:227	n-HAp	223:227	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	2	3	used	utilized	311:318	arg2	powder					304:309	n-HAp powder	298:309	n-HAp powder	298:309	n-HAp powder utilized as a promising defluoridating material, but it causes a significant pressure drop during field applications.
25129795	1	4	theme	batch	286:290	arg1	mode					292:295	batch mode	286:295	batch mode	286:295	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	4	5	contain	possess	688:694	arg1	n-HAp					637:641	n-HAp	637:641	n-HAp	637:641	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	5	contain	possess	688:694	arg2	DC					696:697	DC	696:697	DC of 1296 and 680 mg F(-)/kg	696:724	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	5	contain	possess	688:694	arg1	composite					672:680	calcium alginate (CaAlg) composite	647:680	calcium alginate (CaAlg) composite	647:680	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	6	theme	CaAlg	665:669	arg1	composite					672:680	calcium alginate (CaAlg) composite	647:680	calcium alginate (CaAlg) composite	647:680	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	8	7	from	conditions	1093:1102	arg1	suitability					1052:1062	The suitability	1048:1062	The suitability of the biocomposite at field conditions	1048:1102	The suitability of the biocomposite at field conditions was also tested.
25129795	6	8	theme	contact	876:882	arg1	time					884:887	contact time	876:887	contact time	876:887	The various adsorption influencing parameters like contact time, pH, co-anions, initial fluoride concentration and temperature were optimized.
25129795	4	9	theme	/kg	722:724	arg1	DC					696:697	DC	696:697	DC of 1296 and 680 mg F(-)/kg	696:724	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	10	dep	F	718:718	arg1	mg					715:716	mg	715:716	mg	715:716	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	11	theme	defluoridation	513:526	arg1	DC					538:539	DC	538:539	DC	538:539	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	11	theme	defluoridation	513:526	arg1	capacity					528:535	The defluoridation capacity	509:535	The defluoridation capacity (DC) of synthesized n-HApAlg composite	509:574	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	3	12	theme	n-HApAlg	473:480	arg1	composite					482:490	n-HApAlg composite	473:490	n-HApAlg composite	473:490	To overcome such technological bottlenecks, n-HApAlg composite was synthesized.
25129795	2	13	theme	n-HAp	298:302	arg1	powder					304:309	n-HAp powder	298:309	n-HAp powder	298:309	n-HAp powder utilized as a promising defluoridating material, but it causes a significant pressure drop during field applications.
25129795	1	14	theme	bioencapsulating	185:200	arg1	nano-hydroxyapatite					202:220	alginate (Alg) bioencapsulating nano-hydroxyapatite	170:220	alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely	170:235	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	1	14	theme	bioencapsulating	185:200	arg1	n-HAp					223:227	n-HAp	223:227	n-HAp	223:227	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	6	15	theme	influencing	848:858	arg1	parameters					860:869	The various adsorption influencing parameters	825:869	The various adsorption influencing parameters like contact time, pH, co-anions, initial fluoride concentration and temperature	825:950	The various adsorption influencing parameters like contact time, pH, co-anions, initial fluoride concentration and temperature were optimized.
25129795	4	16	dep	mg	715:716	arg1	680					711:713	680	711:713	680	711:713	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	16	dep	mg	715:716	arg1	1296					702:705	1296	702:705	1296	702:705	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	7	17	theme	kinetic	1032:1038	arg1	models					1040:1045	kinetic models	1032:1045	kinetic models	1032:1045	The adsorption process was enlightened by various isotherms and kinetic models.
25129795	2	18	theme	field	409:413	arg1	applications					415:426	field applications	409:426	field applications	409:426	n-HAp powder utilized as a promising defluoridating material, but it causes a significant pressure drop during field applications.
25129795	8	19	theme	field	1087:1091	arg1	conditions					1093:1102	field conditions	1087:1102	field conditions	1087:1102	The suitability of the biocomposite at field conditions was also tested.
25129795	2	20	theme	defluoridating	335:348	arg1	material					350:357	a promising defluoridating material	323:357	a promising defluoridating material	323:357	n-HAp powder utilized as a promising defluoridating material, but it causes a significant pressure drop during field applications.
25129795	6	21	theme	various	829:835	arg1	parameters					860:869	The various adsorption influencing parameters	825:869	The various adsorption influencing parameters like contact time, pH, co-anions, initial fluoride concentration and temperature	825:950	The various adsorption influencing parameters like contact time, pH, co-anions, initial fluoride concentration and temperature were optimized.
25129795	4	22	theme	F	718:718	arg1	/kg					722:724	1296 and 680 mg F(-)/kg	702:724	1296 and 680 mg F(-)/kg	702:724	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	2	23	theme	promising	325:333	arg1	material					350:357	a promising defluoridating material	323:357	a promising defluoridating material	323:357	n-HAp powder utilized as a promising defluoridating material, but it causes a significant pressure drop during field applications.
25129795	0	24	theme	alginate	13:20	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of alginate	0:20	Synthesis of alginate bioencapsulated nano-hydroxyapatite composite for selective fluoride sorption.
25129795	4	25	theme	mg	609:610	arg1	F					612:612	3870 mg F	604:612	3870 mg F	604:612	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	6	26	theme	adsorption	837:846	arg1	parameters					860:869	The various adsorption influencing parameters	825:869	The various adsorption influencing parameters like contact time, pH, co-anions, initial fluoride concentration and temperature	825:950	The various adsorption influencing parameters like contact time, pH, co-anions, initial fluoride concentration and temperature were optimized.
25129795	4	27	theme	F	612:612	arg1	/kg					616:618	3870 mg F(-)/kg	604:618	3870 mg F(-)/kg	604:618	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	3	28	theme	such	441:444	arg1	bottlenecks					460:470	such technological bottlenecks	441:470	such technological bottlenecks	441:470	To overcome such technological bottlenecks, n-HApAlg composite was synthesized.
25129795	4	29	theme	3870	604:607	arg1	mg					609:610	mg	609:610	mg	609:610	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	5	30	theme	EDAX	810:813	arg1	analysis					815:822	EDAX analysis	810:822	EDAX analysis	810:822	The biocomposite features were characterized using FTIR and SEM with EDAX analysis.
25129795	4	31	theme	alginate	655:662	arg1	composite					672:680	calcium alginate (CaAlg) composite	647:680	calcium alginate (CaAlg) composite	647:680	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	0	32	theme	nano-hydroxyapatite	38:56	arg1	composite					58:66	nano-hydroxyapatite composite	38:66	nano-hydroxyapatite composite	38:66	Synthesis of alginate bioencapsulated nano-hydroxyapatite composite for selective fluoride sorption.
25129795	6	33	theme	fluoride	913:920	arg1	concentration					922:934	initial fluoride concentration	905:934	initial fluoride concentration	905:934	The various adsorption influencing parameters like contact time, pH, co-anions, initial fluoride concentration and temperature were optimized.
25129795	3	34	theme	technological	446:458	arg1	bottlenecks					460:470	such technological bottlenecks	441:470	such technological bottlenecks	441:470	To overcome such technological bottlenecks, n-HApAlg composite was synthesized.
25129795	1	35	theme	n-HApAlg	237:244	arg1	composite					246:254	eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite	144:254	eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode	144:295	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	0	36	theme	selective	72:80	arg1	sorption					91:98	selective fluoride sorption	72:98	selective fluoride sorption	72:98	Synthesis of alginate bioencapsulated nano-hydroxyapatite composite for selective fluoride sorption.
25129795	4	37	theme	composite	566:574	arg1	DC					538:539	DC	538:539	DC	538:539	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	37	theme	composite	566:574	arg1	capacity					528:535	The defluoridation capacity	509:535	The defluoridation capacity (DC) of synthesized n-HApAlg composite	509:574	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	38	theme	synthesized	545:555	arg1	composite					566:574	synthesized n-HApAlg composite	545:574	synthesized n-HApAlg composite	545:574	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	39	theme	enhanced	589:596	arg1	DC					598:599	an enhanced DC	586:599	an enhanced DC of 3870 mg F(-)/kg	586:618	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	1	40	from	studies	275:281	arg1	mode					292:295	batch mode	286:295	batch mode	286:295	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	1	41	theme	alginate	170:177	arg1	nano-hydroxyapatite					202:220	alginate (Alg) bioencapsulating nano-hydroxyapatite	170:220	alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely	170:235	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	1	41	theme	alginate	170:177	arg1	n-HAp					223:227	n-HAp	223:227	n-HAp	223:227	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	7	42	theme	various	1010:1016	arg1	isotherms					1018:1026	various isotherms	1010:1026	various isotherms	1010:1026	The adsorption process was enlightened by various isotherms and kinetic models.
25129795	1	43	theme	composite	246:254	arg1	development					129:139	the development	125:139	the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode	125:295	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	4	44	contain	possesses	576:584	arg1	DC					538:539	DC	538:539	DC	538:539	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	44	contain	possesses	576:584	arg1	capacity					528:535	The defluoridation capacity	509:535	The defluoridation capacity (DC) of synthesized n-HApAlg composite	509:574	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	44	contain	possesses	576:584	arg2	DC					598:599	an enhanced DC	586:599	an enhanced DC of 3870 mg F(-)/kg	586:618	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	1	45	theme	adsorbent	157:165	arg1	composite					246:254	eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite	144:254	eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode	144:295	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	4	46	theme	/kg	616:618	arg1	DC					598:599	an enhanced DC	586:599	an enhanced DC of 3870 mg F(-)/kg	586:618	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	4	47	theme	calcium	647:653	arg1	composite					672:680	calcium alginate (CaAlg) composite	647:680	calcium alginate (CaAlg) composite	647:680	The defluoridation capacity (DC) of synthesized n-HApAlg composite possesses an enhanced DC of 3870 mg F(-)/kg when compared to n-HAp and calcium alginate (CaAlg) composite which possess DC of 1296 and 680 mg F(-)/kg, respectively.
25129795	7	48	theme	adsorption	972:981	arg1	process					983:989	The adsorption process	968:989	The adsorption process	968:989	The adsorption process was enlightened by various isotherms and kinetic models.
25129795	1	49	theme	defluoridation	260:273	arg1	studies					275:281	defluoridation studies	260:281	defluoridation studies in batch mode	260:295	This article focuses on the development of eco-friendly adsorbent by alginate (Alg) bioencapsulating nano-hydroxyapatite (n-HAp) namely n-HApAlg composite for defluoridation studies in batch mode.
25129795	0	50	theme	fluoride	82:89	arg1	sorption					91:98	selective fluoride sorption	72:98	selective fluoride sorption	72:98	Synthesis of alginate bioencapsulated nano-hydroxyapatite composite for selective fluoride sorption.
25129795	5	51	theme	biocomposite	745:756	arg1	features					758:765	The biocomposite features	741:765	The biocomposite features	741:765	The biocomposite features were characterized using FTIR and SEM with EDAX analysis.
25129795	2	52	theme	pressure	388:395	arg1	drop					397:400	a significant pressure drop	374:400	a significant pressure drop during field applications	374:426	n-HAp powder utilized as a promising defluoridating material, but it causes a significant pressure drop during field applications.
25129795	8	53	theme	biocomposite	1071:1082	arg1	suitability					1052:1062	The suitability	1048:1062	The suitability of the biocomposite at field conditions	1048:1102	The suitability of the biocomposite at field conditions was also tested.
25129795	6	54	theme	initial	905:911	arg1	concentration					922:934	initial fluoride concentration	905:934	initial fluoride concentration	905:934	The various adsorption influencing parameters like contact time, pH, co-anions, initial fluoride concentration and temperature were optimized.
28153298	0	0	theme	composite	87:95	arg1	electrode					119:127	Ta2O5-chitosan composite modified carbon paste electrode	72:127	Ta2O5-chitosan composite modified carbon paste electrode	72:127	Sensitive, simultaneous determination of chrysin and baicalein based on Ta2O5-chitosan composite modified carbon paste electrode.
28153298	3	1	theme	cyclic	656:661	arg1	CV					676:677	CV	676:677	CV	676:677	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	1	theme	cyclic	656:661	arg1	voltammetry					663:673	cyclic voltammetry	656:673	cyclic voltammetry (CV)	656:678	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	1	2	theme	major	160:164	arg1	baicalein					142:150	baicalein	142:150	baicalein	142:150	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	1	2	theme	major	160:164	arg1	Chrysin					130:136	Chrysin	130:136	Chrysin	130:136	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	1	2	theme	major	160:164	arg1	flavonoids					166:175	the major flavonoids	156:175	the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM)	156:258	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	0	3	theme	Ta2O5-chitosan	72:85	arg1	electrode					119:127	Ta2O5-chitosan composite modified carbon paste electrode	72:127	Ta2O5-chitosan composite modified carbon paste electrode	72:127	Sensitive, simultaneous determination of chrysin and baicalein based on Ta2O5-chitosan composite modified carbon paste electrode.
28153298	6	4	theme	excellent	1212:1220	arg1	stability					1222:1230	excellent stability	1212:1230	excellent stability	1212:1230	Furthermore, the proposed electrochemical sensor displayed high sensitivity, excellent stability and got satisfactory results in oroxylum indicum samples analysis.
28153298	2	5	theme	great	337:341	arg1	challenge					343:351	a great challenge	335:351	a great challenge to detect both of them simultaneously	335:389	Owing to their similar characteristics and physiochemical property, it is a great challenge to detect both of them simultaneously.
28153298	2	5	theme	great	337:341	arg1	it					329:330	it	329:330	it	329:330	Owing to their similar characteristics and physiochemical property, it is a great challenge to detect both of them simultaneously.
28153298	0	6	theme	carbon	106:111	arg1	electrode					119:127	Ta2O5-chitosan composite modified carbon paste electrode	72:127	Ta2O5-chitosan composite modified carbon paste electrode	72:127	Sensitive, simultaneous determination of chrysin and baicalein based on Ta2O5-chitosan composite modified carbon paste electrode.
28153298	2	7	theme	similar	276:282	arg1	characteristics					284:298	their similar characteristics	270:298	their similar characteristics	270:298	Owing to their similar characteristics and physiochemical property, it is a great challenge to detect both of them simultaneously.
28153298	0	8	theme	modified	97:104	arg1	electrode					119:127	Ta2O5-chitosan composite modified carbon paste electrode	72:127	Ta2O5-chitosan composite modified carbon paste electrode	72:127	Sensitive, simultaneous determination of chrysin and baicalein based on Ta2O5-chitosan composite modified carbon paste electrode.
28153298	4	9	dep	determination	946:958	arg1	both					960:963	both	960:963	both	960:963	Under the optimum conditions, the prepared Ta2O5-CTS-CPE exhibited excellent electrocatalytic activity toward the oxidation of chrysin and baicalein, and it could serve as the sensor for highly sensitive and simultaneous determination both of them.
28153298	5	10	theme	detection	1031:1039	arg1	limits					1041:1046	the detection limits	1027:1046	the detection limits	1027:1046	The linear range is 0.08-4.0μM for both of them, and the detection limits were determined to be 0.03 and 0.05μM (S/N=3) for chrysin and baicalein, respectively.
28153298	5	10	theme	detection	1031:1039	arg1	0.03					1070:1073	0.03	1070:1073	0.03	1070:1073	The linear range is 0.08-4.0μM for both of them, and the detection limits were determined to be 0.03 and 0.05μM (S/N=3) for chrysin and baicalein, respectively.
28153298	4	11	theme	excellent	792:800	arg1	activity					819:826	excellent electrocatalytic activity	792:826	excellent electrocatalytic activity toward the oxidation of chrysin and baicalein	792:872	Under the optimum conditions, the prepared Ta2O5-CTS-CPE exhibited excellent electrocatalytic activity toward the oxidation of chrysin and baicalein, and it could serve as the sensor for highly sensitive and simultaneous determination both of them.
28153298	3	12	theme	oxide	415:419	arg1	particles					429:437	tantalum oxide (Ta2O5) particles	406:437	tantalum oxide (Ta2O5) particles	406:437	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	0	13	theme	paste	113:117	arg1	electrode					119:127	Ta2O5-chitosan composite modified carbon paste electrode	72:127	Ta2O5-chitosan composite modified carbon paste electrode	72:127	Sensitive, simultaneous determination of chrysin and baicalein based on Ta2O5-chitosan composite modified carbon paste electrode.
28153298	3	14	theme	electron	543:550	arg1	spectroscopy					588:599	X-ray diffraction spectroscopy	570:599	X-ray diffraction spectroscopy (XRD)	570:605	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	14	theme	electron	543:550	arg1	SEM					564:566	SEM	564:566	SEM	564:566	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	14	theme	electron	543:550	arg1	microscope					552:561	scanning electron microscope	534:561	scanning electron microscope (SEM)	534:567	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	6	15	theme	satisfactory	1240:1251	arg1	results					1253:1259	satisfactory results	1240:1259	satisfactory results	1240:1259	Furthermore, the proposed electrochemical sensor displayed high sensitivity, excellent stability and got satisfactory results in oroxylum indicum samples analysis.
28153298	1	16	theme	oroxylum	186:193	arg1	indicum					195:201	oroxylum indicum	186:201	oroxylum indicum	186:201	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	1	16	theme	oroxylum	186:193	arg1	herb					217:220	an essential herb	204:220	an essential herb in traditional Chinese medicine (TCM)	204:258	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	0	17	theme	Sensitive	0:8	arg1	determination					24:36	Sensitive, simultaneous determination	0:36	Sensitive, simultaneous determination of chrysin and baicalein	0:61	Sensitive, simultaneous determination of chrysin and baicalein based on Ta2O5-chitosan composite modified carbon paste electrode.
28153298	3	18	theme	impedance	700:708	arg1	EIS					719:721	EIS	719:721	EIS	719:721	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	18	theme	impedance	700:708	arg1	spectrum					710:717	electrochemical impedance spectrum	684:717	electrochemical impedance spectrum(EIS)	684:722	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	4	19	theme	electrocatalytic	802:817	arg1	activity					819:826	excellent electrocatalytic activity	792:826	excellent electrocatalytic activity toward the oxidation of chrysin and baicalein	792:872	Under the optimum conditions, the prepared Ta2O5-CTS-CPE exhibited excellent electrocatalytic activity toward the oxidation of chrysin and baicalein, and it could serve as the sensor for highly sensitive and simultaneous determination both of them.
28153298	2	20	theme	physiochemical	304:317	arg1	property					319:326	physiochemical property	304:326	physiochemical property	304:326	Owing to their similar characteristics and physiochemical property, it is a great challenge to detect both of them simultaneously.
28153298	3	21	theme	tantalum	406:413	arg1	oxide					415:419	tantalum oxide	406:419	tantalum oxide (Ta2O5) particles	406:437	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	21	theme	tantalum	406:413	arg1	Ta2O5					422:426	Ta2O5	422:426	Ta2O5	422:426	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	6	22	theme	samples	1281:1287	arg1	analysis					1289:1296	oroxylum indicum samples analysis	1264:1296	oroxylum indicum samples analysis	1264:1296	Furthermore, the proposed electrochemical sensor displayed high sensitivity, excellent stability and got satisfactory results in oroxylum indicum samples analysis.
28153298	4	23	theme	optimum	735:741	arg1	conditions					743:752	the optimum conditions	731:752	the optimum conditions	731:752	Under the optimum conditions, the prepared Ta2O5-CTS-CPE exhibited excellent electrocatalytic activity toward the oxidation of chrysin and baicalein, and it could serve as the sensor for highly sensitive and simultaneous determination both of them.
28153298	6	24	theme	indicum	1273:1279	arg1	analysis					1289:1296	oroxylum indicum samples analysis	1264:1296	oroxylum indicum samples analysis	1264:1296	Furthermore, the proposed electrochemical sensor displayed high sensitivity, excellent stability and got satisfactory results in oroxylum indicum samples analysis.
28153298	3	25	theme	scanning	534:541	arg1	spectroscopy					588:599	X-ray diffraction spectroscopy	570:599	X-ray diffraction spectroscopy (XRD)	570:605	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	25	theme	scanning	534:541	arg1	SEM					564:566	SEM	564:566	SEM	564:566	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	25	theme	scanning	534:541	arg1	microscope					552:561	scanning electron microscope	534:561	scanning electron microscope (SEM)	534:567	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	0	26	theme	chrysin	41:47	arg1	determination					24:36	Sensitive, simultaneous determination	0:36	Sensitive, simultaneous determination of chrysin and baicalein	0:61	Sensitive, simultaneous determination of chrysin and baicalein based on Ta2O5-chitosan composite modified carbon paste electrode.
28153298	3	27	theme	electrochemical	684:698	arg1	EIS					719:721	EIS	719:721	EIS	719:721	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	27	theme	electrochemical	684:698	arg1	spectrum					710:717	electrochemical impedance spectrum	684:717	electrochemical impedance spectrum(EIS)	684:722	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	6	28	theme	oroxylum	1264:1271	arg1	analysis					1289:1296	oroxylum indicum samples analysis	1264:1296	oroxylum indicum samples analysis	1264:1296	Furthermore, the proposed electrochemical sensor displayed high sensitivity, excellent stability and got satisfactory results in oroxylum indicum samples analysis.
28153298	1	29	located	found	177:181	arg2	flavonoids					166:175	the major flavonoids	156:175	the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM)	156:258	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	1	29	located	found	177:181	arg1	indicum					195:201	oroxylum indicum	186:201	oroxylum indicum	186:201	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	1	29	located	found	177:181	arg2	baicalein					142:150	baicalein	142:150	baicalein	142:150	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	1	29	located	found	177:181	arg2	Chrysin					130:136	Chrysin	130:136	Chrysin	130:136	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	1	29	located	found	177:181	arg1	herb					217:220	an essential herb	204:220	an essential herb in traditional Chinese medicine (TCM)	204:258	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	6	30	theme	proposed	1152:1159	arg1	sensor					1177:1182	the proposed electrochemical sensor	1148:1182	the proposed electrochemical sensor	1148:1182	Furthermore, the proposed electrochemical sensor displayed high sensitivity, excellent stability and got satisfactory results in oroxylum indicum samples analysis.
28153298	1	31	theme	essential	207:215	arg1	indicum					195:201	oroxylum indicum	186:201	oroxylum indicum	186:201	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	1	31	theme	essential	207:215	arg1	herb					217:220	an essential herb	204:220	an essential herb in traditional Chinese medicine (TCM)	204:258	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	0	32	theme	baicalein	53:61	arg1	determination					24:36	Sensitive, simultaneous determination	0:36	Sensitive, simultaneous determination of chrysin and baicalein	0:61	Sensitive, simultaneous determination of chrysin and baicalein based on Ta2O5-chitosan composite modified carbon paste electrode.
28153298	5	33	theme	linear	978:983	arg1	0.08-4.0μM					994:1003	0.08-4.0μM	994:1003	0.08-4.0μM	994:1003	The linear range is 0.08-4.0μM for both of them, and the detection limits were determined to be 0.03 and 0.05μM (S/N=3) for chrysin and baicalein, respectively.
28153298	5	33	theme	linear	978:983	arg1	range					985:989	The linear range	974:989	The linear range	974:989	The linear range is 0.08-4.0μM for both of them, and the detection limits were determined to be 0.03 and 0.05μM (S/N=3) for chrysin and baicalein, respectively.
28153298	1	34	from	herb	217:220	arg1	TCM					255:257	TCM	255:257	TCM	255:257	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	1	34	from	herb	217:220	arg1	medicine					245:252	traditional Chinese medicine	225:252	traditional Chinese medicine (TCM)	225:258	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	3	35	theme	carbon	461:466	arg1	Ta2O5-CTS-CPE					485:497	Ta2O5-CTS-CPE	485:497	Ta2O5-CTS-CPE	485:497	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	35	theme	carbon	461:466	arg1	electrode					474:482	chitosan modified carbon paste electrode	443:482	chitosan modified carbon paste electrode (Ta2O5-CTS-CPE)	443:498	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	6	36	theme	electrochemical	1161:1175	arg1	sensor					1177:1182	the proposed electrochemical sensor	1148:1182	the proposed electrochemical sensor	1148:1182	Furthermore, the proposed electrochemical sensor displayed high sensitivity, excellent stability and got satisfactory results in oroxylum indicum samples analysis.
28153298	4	37	theme	chrysin	852:858	arg1	oxidation					839:847	the oxidation	835:847	the oxidation of chrysin and baicalein	835:872	Under the optimum conditions, the prepared Ta2O5-CTS-CPE exhibited excellent electrocatalytic activity toward the oxidation of chrysin and baicalein, and it could serve as the sensor for highly sensitive and simultaneous determination both of them.
28153298	4	38	theme	prepared	759:766	arg1	Ta2O5-CTS-CPE					768:780	the prepared Ta2O5-CTS-CPE	755:780	the prepared Ta2O5-CTS-CPE	755:780	Under the optimum conditions, the prepared Ta2O5-CTS-CPE exhibited excellent electrocatalytic activity toward the oxidation of chrysin and baicalein, and it could serve as the sensor for highly sensitive and simultaneous determination both of them.
28153298	3	39	theme	paste	468:472	arg1	Ta2O5-CTS-CPE					485:497	Ta2O5-CTS-CPE	485:497	Ta2O5-CTS-CPE	485:497	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	39	theme	paste	468:472	arg1	electrode					474:482	chitosan modified carbon paste electrode	443:482	chitosan modified carbon paste electrode (Ta2O5-CTS-CPE)	443:498	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	40	theme	diffraction	576:586	arg1	XRD					602:604	XRD	602:604	XRD	602:604	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	40	theme	diffraction	576:586	arg1	spectroscopy					588:599	X-ray diffraction spectroscopy	570:599	X-ray diffraction spectroscopy (XRD)	570:605	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	40	theme	diffraction	576:586	arg1	microscope					552:561	scanning electron microscope	534:561	scanning electron microscope (SEM)	534:567	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	41	theme	chitosan	443:450	arg1	Ta2O5-CTS-CPE					485:497	Ta2O5-CTS-CPE	485:497	Ta2O5-CTS-CPE	485:497	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	41	theme	chitosan	443:450	arg1	electrode					474:482	chitosan modified carbon paste electrode	443:482	chitosan modified carbon paste electrode (Ta2O5-CTS-CPE)	443:498	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	4	42	theme	baicalein	864:872	arg1	oxidation					839:847	the oxidation	835:847	the oxidation of chrysin and baicalein	835:872	Under the optimum conditions, the prepared Ta2O5-CTS-CPE exhibited excellent electrocatalytic activity toward the oxidation of chrysin and baicalein, and it could serve as the sensor for highly sensitive and simultaneous determination both of them.
28153298	6	43	theme	high	1194:1197	arg1	sensitivity					1199:1209	high sensitivity	1194:1209	high sensitivity	1194:1209	Furthermore, the proposed electrochemical sensor displayed high sensitivity, excellent stability and got satisfactory results in oroxylum indicum samples analysis.
28153298	3	44	theme	modified	452:459	arg1	Ta2O5-CTS-CPE					485:497	Ta2O5-CTS-CPE	485:497	Ta2O5-CTS-CPE	485:497	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	44	theme	modified	452:459	arg1	electrode					474:482	chitosan modified carbon paste electrode	443:482	chitosan modified carbon paste electrode (Ta2O5-CTS-CPE)	443:498	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	1	45	theme	traditional	225:235	arg1	TCM					255:257	TCM	255:257	TCM	255:257	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	1	45	theme	traditional	225:235	arg1	medicine					245:252	traditional Chinese medicine	225:252	traditional Chinese medicine (TCM)	225:258	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	4	46	theme	sensitive	919:927	arg1	determination					946:958	highly sensitive and simultaneous determination	912:958	highly sensitive and simultaneous determination	912:958	Under the optimum conditions, the prepared Ta2O5-CTS-CPE exhibited excellent electrocatalytic activity toward the oxidation of chrysin and baicalein, and it could serve as the sensor for highly sensitive and simultaneous determination both of them.
28153298	3	47	theme	infrared	626:633	arg1	FTIR					649:652	FTIR	649:652	FTIR	649:652	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	47	theme	infrared	626:633	arg1	spectroscopy					635:646	infrared spectroscopy	626:646	infrared spectroscopy (FTIR)	626:653	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	48	theme	X-ray	570:574	arg1	XRD					602:604	XRD	602:604	XRD	602:604	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	48	theme	X-ray	570:574	arg1	spectroscopy					588:599	X-ray diffraction spectroscopy	570:599	X-ray diffraction spectroscopy (XRD)	570:605	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	3	48	theme	X-ray	570:574	arg1	microscope					552:561	scanning electron microscope	534:561	scanning electron microscope (SEM)	534:567	In this work, tantalum oxide (Ta2O5) particles and chitosan modified carbon paste electrode (Ta2O5-CTS-CPE) was prepared and characterized by scanning electron microscope (SEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectrum(EIS).
28153298	1	49	theme	Chinese	237:243	arg1	TCM					255:257	TCM	255:257	TCM	255:257	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	1	49	theme	Chinese	237:243	arg1	medicine					245:252	traditional Chinese medicine	225:252	traditional Chinese medicine (TCM)	225:258	Chrysin and baicalein are the major flavonoids found in oroxylum indicum, an essential herb in traditional Chinese medicine (TCM).
28153298	4	50	theme	simultaneous	933:944	arg1	determination					946:958	highly sensitive and simultaneous determination	912:958	highly sensitive and simultaneous determination	912:958	Under the optimum conditions, the prepared Ta2O5-CTS-CPE exhibited excellent electrocatalytic activity toward the oxidation of chrysin and baicalein, and it could serve as the sensor for highly sensitive and simultaneous determination both of them.
28153298	0	51	dep	Sensitive	0:8	arg1	simultaneous					11:22	simultaneous	11:22	simultaneous	11:22	Sensitive, simultaneous determination of chrysin and baicalein based on Ta2O5-chitosan composite modified carbon paste electrode.
28153298	6	52	dep	sensitivity	1199:1209	arg1	results					1253:1259	satisfactory results	1240:1259	satisfactory results	1240:1259	Furthermore, the proposed electrochemical sensor displayed high sensitivity, excellent stability and got satisfactory results in oroxylum indicum samples analysis.
25817660	6	0	theme	better	1032:1037	arg1	adhesion					1051:1058	a better interfacial adhesion	1030:1058	a better interfacial adhesion for CNF based nanocomposites compared to CNC	1030:1103	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
25817660	6	1	theme	solubility	942:951	arg1	parameters					953:962	the solubility parameters	938:962	the solubility parameters of the nanofiller surface polymers and of the PU segments	938:1020	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
25817660	5	2	theme	CNF	864:866	arg1	ratio					855:859	the higher aspect ratio	837:859	the higher aspect ratio of CNF	837:866	While comparing both nanofillers, the improvement in mechanical and thermal properties was more pronounced for the nanocomposites based on CNF which could be explained, not only by the higher aspect ratio of CNF, but also by their better dispersion in the PU matrix.
25817660	5	3	from	improvement	694:704	arg1	properties					732:741	mechanical and thermal properties	709:741	mechanical and thermal properties	709:741	While comparing both nanofillers, the improvement in mechanical and thermal properties was more pronounced for the nanocomposites based on CNF which could be explained, not only by the higher aspect ratio of CNF, but also by their better dispersion in the PU matrix.
25817660	2	4	used	used	352:355	arg2	types					328:332	Two types	324:332	Two types of nanofiber	324:345	Two types of nanofiber were used: cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs).
25817660	6	5	theme	polymers	990:997	arg1	parameters					953:962	the solubility parameters	938:962	the solubility parameters of the nanofiller surface polymers and of the PU segments	938:1020	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
25817660	5	6	theme	mechanical	709:718	arg1	properties					732:741	mechanical and thermal properties	709:741	mechanical and thermal properties	709:741	While comparing both nanofillers, the improvement in mechanical and thermal properties was more pronounced for the nanocomposites based on CNF which could be explained, not only by the higher aspect ratio of CNF, but also by their better dispersion in the PU matrix.
25817660	5	7	theme	PU	912:913	arg1	matrix					915:920	the PU matrix	908:920	the PU matrix	908:920	While comparing both nanofillers, the improvement in mechanical and thermal properties was more pronounced for the nanocomposites based on CNF which could be explained, not only by the higher aspect ratio of CNF, but also by their better dispersion in the PU matrix.
25817660	2	8	theme	nanofiber	337:345	arg1	types					328:332	Two types	324:332	Two types of nanofiber	324:345	Two types of nanofiber were used: cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs).
25817660	4	9	theme	good	603:606	arg1	properties					619:628	good mechanical properties	603:628	good mechanical properties	603:628	Bionanocomposites presented good mechanical properties in comparison to neat PU.
25817660	6	10	theme	based	1068:1072	arg1	nanocomposites					1074:1087	CNF based nanocomposites	1064:1087	CNF based nanocomposites	1064:1087	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
25817660	0	11	theme	interface	108:116	arg1	effect					98:103	the effect	94:103	the effect of interface	94:116	Bio-based polyurethane reinforced with cellulose nanofibers: a comprehensive investigation on the effect of interface.
25817660	6	12	theme	CNF	1064:1066	arg1	nanocomposites					1074:1087	CNF based nanocomposites	1064:1087	CNF based nanocomposites	1064:1087	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
25817660	3	13	theme	nanocomposite	467:479	arg1	films					481:485	the nanocomposite films	463:485	the nanocomposite films	463:485	The mechanical and thermal properties of the nanocomposite films were studied by DMA, DSC, and tensile tests and the morphology was investigated by SEM.
25817660	1	14	theme	cellulose	180:188	arg1	nanofiller					190:199	cellulose nanofiller	180:199	cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU	180:284	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	6	15	theme	PU	1010:1011	arg1	segments					1013:1020	the PU segments	1006:1020	the PU segments	1006:1020	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
25817660	0	16	theme	Bio-based	0:8	arg1	polyurethane					10:21	Bio-based polyurethane	0:21	Bio-based polyurethane	0:21	Bio-based polyurethane reinforced with cellulose nanofibers: a comprehensive investigation on the effect of interface.
25817660	1	17	theme	diol	272:275	arg1	PU					283:284	diol based PU	272:284	diol based PU	272:284	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	5	18	theme	better	887:892	arg1	dispersion					894:903	their better dispersion	881:903	their better dispersion in the PU matrix	881:920	While comparing both nanofillers, the improvement in mechanical and thermal properties was more pronounced for the nanocomposites based on CNF which could be explained, not only by the higher aspect ratio of CNF, but also by their better dispersion in the PU matrix.
25817660	4	19	theme	neat	647:650	arg1	PU					652:653	neat PU	647:653	neat PU	647:653	Bionanocomposites presented good mechanical properties in comparison to neat PU.
25817660	1	20	theme	based	277:281	arg1	PU					283:284	diol based PU	272:284	diol based PU	272:284	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	3	21	theme	tensile	517:523	arg1	tests					525:529	tensile tests	517:529	tensile tests	517:529	The mechanical and thermal properties of the nanocomposite films were studied by DMA, DSC, and tensile tests and the morphology was investigated by SEM.
25817660	3	22	theme	thermal	441:447	arg1	properties					449:458	The mechanical and thermal properties	422:458	The mechanical and thermal properties of the nanocomposite films	422:485	The mechanical and thermal properties of the nanocomposite films were studied by DMA, DSC, and tensile tests and the morphology was investigated by SEM.
25817660	1	23	theme	PU	149:150	arg1	nanocomposites					153:166	Novel bio-based polyurethane (PU) nanocomposites	119:166	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU	119:284	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	3	24	theme	films	481:485	arg1	properties					449:458	The mechanical and thermal properties	422:458	The mechanical and thermal properties of the nanocomposite films	422:485	The mechanical and thermal properties of the nanocomposite films were studied by DMA, DSC, and tensile tests and the morphology was investigated by SEM.
25817660	3	25	theme	mechanical	426:435	arg1	properties					449:458	The mechanical and thermal properties	422:458	The mechanical and thermal properties of the nanocomposite films	422:485	The mechanical and thermal properties of the nanocomposite films were studied by DMA, DSC, and tensile tests and the morphology was investigated by SEM.
25817660	5	26	theme	higher	841:846	arg1	ratio					855:859	the higher aspect ratio	837:859	the higher aspect ratio of CNF	837:866	While comparing both nanofillers, the improvement in mechanical and thermal properties was more pronounced for the nanocomposites based on CNF which could be explained, not only by the higher aspect ratio of CNF, but also by their better dispersion in the PU matrix.
25817660	2	27	theme	cellulose	391:399	arg1	CNCs					415:418	CNCs	415:418	CNCs	415:418	Two types of nanofiber were used: cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs).
25817660	2	27	theme	cellulose	391:399	arg1	nanocrystals					401:412	cellulose nanocrystals	391:412	cellulose nanocrystals (CNCs)	391:419	Two types of nanofiber were used: cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs).
25817660	0	28	theme	cellulose	39:47	arg1	nanofibers					49:58	cellulose nanofibers	39:58	cellulose nanofibers	39:58	Bio-based polyurethane reinforced with cellulose nanofibers: a comprehensive investigation on the effect of interface.
25817660	0	29	from	investigation	77:89	arg1	effect					98:103	the effect	94:103	the effect of interface	94:116	Bio-based polyurethane reinforced with cellulose nanofibers: a comprehensive investigation on the effect of interface.
25817660	5	30	theme	thermal	724:730	arg1	properties					732:741	mechanical and thermal properties	709:741	mechanical and thermal properties	709:741	While comparing both nanofillers, the improvement in mechanical and thermal properties was more pronounced for the nanocomposites based on CNF which could be explained, not only by the higher aspect ratio of CNF, but also by their better dispersion in the PU matrix.
25817660	5	31	theme	aspect	848:853	arg1	ratio					855:859	the higher aspect ratio	837:859	the higher aspect ratio of CNF	837:866	While comparing both nanofillers, the improvement in mechanical and thermal properties was more pronounced for the nanocomposites based on CNF which could be explained, not only by the higher aspect ratio of CNF, but also by their better dispersion in the PU matrix.
25817660	1	32	theme	Novel	119:123	arg1	nanocomposites					153:166	Novel bio-based polyurethane (PU) nanocomposites	119:166	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU	119:284	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	4	33	attach	presented	593:601	arg2	Bionanocomposites					575:591	Bionanocomposites	575:591	Bionanocomposites	575:591	Bionanocomposites presented good mechanical properties in comparison to neat PU.
25817660	4	33	attach	presented	593:601	arg1	comparison					633:642	comparison	633:642	comparison to neat PU	633:653	Bionanocomposites presented good mechanical properties in comparison to neat PU.
25817660	1	34	theme	bio-based	125:133	arg1	nanocomposites					153:166	Novel bio-based polyurethane (PU) nanocomposites	119:166	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU	119:284	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	5	35	from	dispersion	894:903	arg1	matrix					915:920	the PU matrix	908:920	the PU matrix	908:920	While comparing both nanofillers, the improvement in mechanical and thermal properties was more pronounced for the nanocomposites based on CNF which could be explained, not only by the higher aspect ratio of CNF, but also by their better dispersion in the PU matrix.
25817660	1	36	dep	rachis	220:225	arg1	PU					283:284	diol based PU	272:284	diol based PU	272:284	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	1	37	theme	polyurethane	135:146	arg1	nanocomposites					153:166	Novel bio-based polyurethane (PU) nanocomposites	119:166	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU	119:284	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	1	38	theme	date	230:233	arg1	tree					240:243	date palm tree	230:243	date palm tree	230:243	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	6	39	theme	interfacial	1039:1049	arg1	adhesion					1051:1058	a better interfacial adhesion	1030:1058	a better interfacial adhesion for CNF based nanocomposites compared to CNC	1030:1103	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
25817660	1	40	theme	palm	235:238	arg1	tree					240:243	date palm tree	230:243	date palm tree	230:243	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	6	41	theme	surface	982:988	arg1	polymers					990:997	the nanofiller surface polymers	967:997	the nanofiller surface polymers	967:997	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
25817660	4	42	theme	mechanical	608:617	arg1	properties					619:628	good mechanical properties	603:628	good mechanical properties	603:628	Bionanocomposites presented good mechanical properties in comparison to neat PU.
25817660	2	43	theme	cellulose	358:366	arg1	CNFs					381:384	CNFs	381:384	CNFs	381:384	Two types of nanofiber were used: cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs).
25817660	2	43	theme	cellulose	358:366	arg1	nanofibrils					368:378	cellulose nanofibrils	358:378	cellulose nanofibrils (CNFs)	358:385	Two types of nanofiber were used: cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs).
25817660	6	44	theme	segments	1013:1020	arg1	parameters					953:962	the solubility parameters	938:962	the solubility parameters of the nanofiller surface polymers and of the PU segments	938:1020	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
25817660	1	45	theme	tree	240:243	arg1	PCL					267:269	PCL	267:269	PCL	267:269	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	1	45	theme	tree	240:243	arg1	rachis					220:225	the rachis	216:225	the rachis of date palm tree	216:243	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	1	45	theme	tree	240:243	arg1	polycaprolactone					249:264	polycaprolactone	249:264	polycaprolactone (PCL)	249:270	Novel bio-based polyurethane (PU) nanocomposites composed of cellulose nanofiller extracted from the rachis of date palm tree and polycaprolactone (PCL) diol based PU were prepared by casting/evaporation.
25817660	0	46	theme	comprehensive	63:75	arg1	investigation					77:89	a comprehensive investigation	61:89	a comprehensive investigation on the effect of interface	61:116	Bio-based polyurethane reinforced with cellulose nanofibers: a comprehensive investigation on the effect of interface.
25817660	6	47	theme	nanofiller	971:980	arg1	polymers					990:997	the nanofiller surface polymers	967:997	the nanofiller surface polymers	967:997	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
25817660	6	48	theme	parameters	953:962	arg1	Calculation					923:933	Calculation	923:933	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments	923:1020	Calculation of the solubility parameters of the nanofiller surface polymers and of the PU segments portend a better interfacial adhesion for CNF based nanocomposites compared to CNC.
27154518	7	0	theme	7.14U/mg	918:925	arg1	protein					927:933	7.14U/mg protein	918:933	7.14U/mg protein	918:933	The Km and Vmax values for immobilized β-glucosidase calculated as 5.55mM and 7.14U/mg protein respectively.
27154518	3	1	theme	glutaraldeyde	541:553	arg1	activation					555:564	glutaraldeyde activation	541:564	glutaraldeyde activation	541:564	β-Glucosidase was covalently immobilized on the composite carrier after glutaraldeyde activation.
27154518	11	2	theme	activity	1282:1289	arg1	activity					1282:1289	its initial activity	1270:1289	its initial activity	1270:1289	After 10 cycles of reuse, immobilized β-glucosidase showed 72.83% of its initial activity.
27154518	11	2	theme	activity	1282:1289	arg1	%					1265:1265	72.83%	1260:1265	72.83% of its initial activity	1260:1289	After 10 cycles of reuse, immobilized β-glucosidase showed 72.83% of its initial activity.
27154518	8	3	theme	free	1019:1022	arg1	enzyme					1024:1029	free enzyme	1019:1029	free enzyme	1019:1029	Immobilized β-glucosidase showed better pH and storage stability than free enzyme.
27154518	0	4	from	nanotubes	77:85	arg1	extracts					125:132	tea extracts	121:132	tea extracts	121:132	Characterization of β-glucosidase immobilized on chitosan-multiwalled carbon nanotubes (MWCNTS) and their application on tea extracts for aroma enhancement.
27154518	8	5	theme	Immobilized	949:959	arg1	β-glucosidase					961:973	Immobilized β-glucosidase	949:973	Immobilized β-glucosidase	949:973	Immobilized β-glucosidase showed better pH and storage stability than free enzyme.
27154518	9	6	theme	initial	1115:1121	arg1	activity					1123:1130	the initial activity	1111:1130	the initial activity	1111:1130	After storage at +4°C for 50days, the immobilized enzyme retained its 68.4% of the initial activity.
27154518	0	7	theme	tea	121:123	arg1	extracts					125:132	tea extracts	121:132	tea extracts	121:132	Characterization of β-glucosidase immobilized on chitosan-multiwalled carbon nanotubes (MWCNTS) and their application on tea extracts for aroma enhancement.
27154518	5	8	theme	Optimum	665:671	arg1	pH					673:674	Optimum pH	665:674	Optimum pH	665:674	Optimum pH was found as pH 6.0 and pH 5.0 for free and immobilized enzyme, respectively.
27154518	5	8	theme	Optimum	665:671	arg1	pH					689:690	pH 6.0	689:694	pH 6.0	689:694	Optimum pH was found as pH 6.0 and pH 5.0 for free and immobilized enzyme, respectively.
27154518	5	8	theme	Optimum	665:671	arg1	pH					700:701	pH 5.0	700:705	pH 5.0	700:705	Optimum pH was found as pH 6.0 and pH 5.0 for free and immobilized enzyme, respectively.
27154518	1	9	theme	chitosan-MWCNTs	201:215	arg1	carrier					217:223	chitosan-MWCNTs carrier	201:223	chitosan-MWCNTs carrier	201:223	β-Glucosidase was covalently immobilized on chitosan-MWCNTs carrier and its aroma enhancement effect in different tea samples was investigated.
27154518	2	10	theme	Chitosan-MWCNTs	301:315	arg1	carrier					317:323	Chitosan-MWCNTs carrier	301:323	Chitosan-MWCNTs carrier	301:323	Chitosan-MWCNTs carrier was prepared by mixing chitosan with MWCNTs (5:1w/w) and characterization of prepared composite carrier was done by FTIR, TGA and SEM analysis.
27154518	11	11	theme	reuse	1220:1224	arg1	cycles					1210:1215	10 cycles	1207:1215	10 cycles of reuse	1207:1224	After 10 cycles of reuse, immobilized β-glucosidase showed 72.83% of its initial activity.
27154518	7	12	theme	immobilized	867:877	arg1	β-glucosidase					879:891	immobilized β-glucosidase	867:891	immobilized β-glucosidase	867:891	The Km and Vmax values for immobilized β-glucosidase calculated as 5.55mM and 7.14U/mg protein respectively.
27154518	2	13	theme	carrier	421:427	arg1	characterization					382:397	characterization	382:397	characterization of prepared composite carrier	382:427	Chitosan-MWCNTs carrier was prepared by mixing chitosan with MWCNTs (5:1w/w) and characterization of prepared composite carrier was done by FTIR, TGA and SEM analysis.
27154518	4	14	theme	conditions	608:617	arg1	optimization					573:584	optimization	573:584	optimization of the immobilization conditions	573:617	After optimization of the immobilization conditions, immobilization yield was achieved as 95.22%.
27154518	7	15	theme	Vmax	851:854	arg1	values					856:861	The Km and Vmax values	840:861	The Km and Vmax values for immobilized β-glucosidase calculated as 5.55mM and 7.14U/mg protein respectively.	840:947	The Km and Vmax values for immobilized β-glucosidase calculated as 5.55mM and 7.14U/mg protein respectively.
27154518	2	16	theme	composite	411:419	arg1	carrier					421:427	prepared composite carrier	402:427	prepared composite carrier	402:427	Chitosan-MWCNTs carrier was prepared by mixing chitosan with MWCNTs (5:1w/w) and characterization of prepared composite carrier was done by FTIR, TGA and SEM analysis.
27154518	2	17	theme	SEM	455:457	arg1	analysis					459:466	FTIR, TGA and SEM analysis	441:466	FTIR, TGA and SEM analysis	441:466	Chitosan-MWCNTs carrier was prepared by mixing chitosan with MWCNTs (5:1w/w) and characterization of prepared composite carrier was done by FTIR, TGA and SEM analysis.
27154518	4	18	theme	immobilization	593:606	arg1	conditions					608:617	the immobilization conditions	589:617	the immobilization conditions	589:617	After optimization of the immobilization conditions, immobilization yield was achieved as 95.22%.
27154518	5	19	theme	immobilized	720:730	arg1	enzyme					732:737	free and immobilized enzyme	711:737	free and immobilized enzyme	711:737	Optimum pH was found as pH 6.0 and pH 5.0 for free and immobilized enzyme, respectively.
27154518	2	20	theme	TGA	447:449	arg1	analysis					459:466	FTIR, TGA and SEM analysis	441:466	FTIR, TGA and SEM analysis	441:466	Chitosan-MWCNTs carrier was prepared by mixing chitosan with MWCNTs (5:1w/w) and characterization of prepared composite carrier was done by FTIR, TGA and SEM analysis.
27154518	0	21	theme	β-glucosidase	20:32	arg1	Characterization					0:15	Characterization	0:15	Characterization of β-glucosidase	0:32	Characterization of β-glucosidase immobilized on chitosan-multiwalled carbon nanotubes (MWCNTS) and their application on tea extracts for aroma enhancement.
27154518	4	22	theme	immobilization	620:633	arg1	%					662:662	95.22%	657:662	95.22%	657:662	After optimization of the immobilization conditions, immobilization yield was achieved as 95.22%.
27154518	4	22	theme	immobilization	620:633	arg1	yield					635:639	immobilization yield	620:639	immobilization yield	620:639	After optimization of the immobilization conditions, immobilization yield was achieved as 95.22%.
27154518	8	23	theme	pH	989:990	arg1	stability					1004:1012	better pH and storage stability	982:1012	better pH and storage stability	982:1012	Immobilized β-glucosidase showed better pH and storage stability than free enzyme.
27154518	7	24	theme	Km	844:845	arg1	values					856:861	The Km and Vmax values	840:861	The Km and Vmax values for immobilized β-glucosidase calculated as 5.55mM and 7.14U/mg protein respectively.	840:947	The Km and Vmax values for immobilized β-glucosidase calculated as 5.55mM and 7.14U/mg protein respectively.
27154518	9	25	theme	activity	1123:1130	arg1	%					1106:1106	68.4%	1102:1106	its 68.4% of the initial activity	1098:1130	After storage at +4°C for 50days, the immobilized enzyme retained its 68.4% of the initial activity.
27154518	9	25	theme	activity	1123:1130	arg1	activity					1123:1130	the initial activity	1111:1130	the initial activity	1111:1130	After storage at +4°C for 50days, the immobilized enzyme retained its 68.4% of the initial activity.
27154518	0	26	theme	chitosan-multiwalled	49:68	arg1	MWCNTS					88:93	MWCNTS	88:93	MWCNTS	88:93	Characterization of β-glucosidase immobilized on chitosan-multiwalled carbon nanotubes (MWCNTS) and their application on tea extracts for aroma enhancement.
27154518	0	26	theme	chitosan-multiwalled	49:68	arg1	nanotubes					77:85	chitosan-multiwalled carbon nanotubes	49:85	chitosan-multiwalled carbon nanotubes (MWCNTS)	49:94	Characterization of β-glucosidase immobilized on chitosan-multiwalled carbon nanotubes (MWCNTS) and their application on tea extracts for aroma enhancement.
27154518	11	27	theme	immobilized	1227:1237	arg1	β-glucosidase					1239:1251	immobilized β-glucosidase	1227:1251	immobilized β-glucosidase	1227:1251	After 10 cycles of reuse, immobilized β-glucosidase showed 72.83% of its initial activity.
27154518	1	28	theme	aroma	233:237	arg1	effect					251:256	its aroma enhancement effect	229:256	its aroma enhancement effect in different tea samples	229:281	β-Glucosidase was covalently immobilized on chitosan-MWCNTs carrier and its aroma enhancement effect in different tea samples was investigated.
27154518	2	29	theme	FTIR	441:444	arg1	analysis					459:466	FTIR, TGA and SEM analysis	441:466	FTIR, TGA and SEM analysis	441:466	Chitosan-MWCNTs carrier was prepared by mixing chitosan with MWCNTs (5:1w/w) and characterization of prepared composite carrier was done by FTIR, TGA and SEM analysis.
27154518	10	30	theme	calculated	1137:1146	arg1	t1/2					1159:1162	t1/2	1159:1162	t1/2	1159:1162	The calculated half-life (t1/2) of immobilized enzyme was 115.8min.
27154518	10	30	theme	calculated	1137:1146	arg1	half-life					1148:1156	The calculated half-life	1133:1156	The calculated half-life (t1/2) of immobilized enzyme	1133:1185	The calculated half-life (t1/2) of immobilized enzyme was 115.8min.
27154518	10	30	theme	calculated	1137:1146	arg1	115.8min					1191:1198	115.8min	1191:1198	115.8min	1191:1198	The calculated half-life (t1/2) of immobilized enzyme was 115.8min.
27154518	0	31	theme	aroma	138:142	arg1	enhancement					144:154	aroma enhancement	138:154	aroma enhancement	138:154	Characterization of β-glucosidase immobilized on chitosan-multiwalled carbon nanotubes (MWCNTS) and their application on tea extracts for aroma enhancement.
27154518	1	32	theme	enhancement	239:249	arg1	effect					251:256	its aroma enhancement effect	229:256	its aroma enhancement effect in different tea samples	229:281	β-Glucosidase was covalently immobilized on chitosan-MWCNTs carrier and its aroma enhancement effect in different tea samples was investigated.
27154518	10	33	theme	enzyme	1180:1185	arg1	t1/2					1159:1162	t1/2	1159:1162	t1/2	1159:1162	The calculated half-life (t1/2) of immobilized enzyme was 115.8min.
27154518	10	33	theme	enzyme	1180:1185	arg1	half-life					1148:1156	The calculated half-life	1133:1156	The calculated half-life (t1/2) of immobilized enzyme	1133:1185	The calculated half-life (t1/2) of immobilized enzyme was 115.8min.
27154518	10	33	theme	enzyme	1180:1185	arg1	115.8min					1191:1198	115.8min	1191:1198	115.8min	1191:1198	The calculated half-life (t1/2) of immobilized enzyme was 115.8min.
27154518	5	34	theme	free	711:714	arg1	enzyme					732:737	free and immobilized enzyme	711:737	free and immobilized enzyme	711:737	Optimum pH was found as pH 6.0 and pH 5.0 for free and immobilized enzyme, respectively.
27154518	3	35	theme	composite	517:525	arg1	carrier					527:533	the composite carrier	513:533	the composite carrier after glutaraldeyde activation	513:564	β-Glucosidase was covalently immobilized on the composite carrier after glutaraldeyde activation.
27154518	0	36	theme	carbon	70:75	arg1	MWCNTS					88:93	MWCNTS	88:93	MWCNTS	88:93	Characterization of β-glucosidase immobilized on chitosan-multiwalled carbon nanotubes (MWCNTS) and their application on tea extracts for aroma enhancement.
27154518	0	36	theme	carbon	70:75	arg1	nanotubes					77:85	chitosan-multiwalled carbon nanotubes	49:85	chitosan-multiwalled carbon nanotubes (MWCNTS)	49:94	Characterization of β-glucosidase immobilized on chitosan-multiwalled carbon nanotubes (MWCNTS) and their application on tea extracts for aroma enhancement.
27154518	10	37	theme	immobilized	1168:1178	arg1	enzyme					1180:1185	immobilized enzyme	1168:1185	immobilized enzyme	1168:1185	The calculated half-life (t1/2) of immobilized enzyme was 115.8min.
27154518	11	38	theme	initial	1274:1280	arg1	activity					1282:1289	its initial activity	1270:1289	its initial activity	1270:1289	After 10 cycles of reuse, immobilized β-glucosidase showed 72.83% of its initial activity.
27154518	6	39	theme	enzyme	781:786	arg1	temperature					762:772	Optimum temperature	754:772	Optimum temperature of the enzyme	754:786	Optimum temperature of the enzyme was shifted from 35°C to 45°C after immobilization.
27154518	1	40	from	effect	251:256	arg1	samples					275:281	different tea samples	261:281	different tea samples	261:281	β-Glucosidase was covalently immobilized on chitosan-MWCNTs carrier and its aroma enhancement effect in different tea samples was investigated.
27154518	8	41	theme	better	982:987	arg1	stability					1004:1012	better pH and storage stability	982:1012	better pH and storage stability	982:1012	Immobilized β-glucosidase showed better pH and storage stability than free enzyme.
27154518	0	42	from	application	106:116	arg1	extracts					125:132	tea extracts	121:132	tea extracts	121:132	Characterization of β-glucosidase immobilized on chitosan-multiwalled carbon nanotubes (MWCNTS) and their application on tea extracts for aroma enhancement.
27154518	8	43	theme	storage	996:1002	arg1	stability					1004:1012	better pH and storage stability	982:1012	better pH and storage stability	982:1012	Immobilized β-glucosidase showed better pH and storage stability than free enzyme.
27154518	2	44	theme	prepared	402:409	arg1	carrier					421:427	prepared composite carrier	402:427	prepared composite carrier	402:427	Chitosan-MWCNTs carrier was prepared by mixing chitosan with MWCNTs (5:1w/w) and characterization of prepared composite carrier was done by FTIR, TGA and SEM analysis.
27154518	9	45	theme	immobilized	1070:1080	arg1	enzyme					1082:1087	the immobilized enzyme	1066:1087	the immobilized enzyme	1066:1087	After storage at +4°C for 50days, the immobilized enzyme retained its 68.4% of the initial activity.
27154518	1	46	theme	different	261:269	arg1	samples					275:281	different tea samples	261:281	different tea samples	261:281	β-Glucosidase was covalently immobilized on chitosan-MWCNTs carrier and its aroma enhancement effect in different tea samples was investigated.
27154518	9	47	from	+4°C	1049:1052	arg1	storage					1038:1044	storage	1038:1044	storage at +4°C for 50days	1038:1063	After storage at +4°C for 50days, the immobilized enzyme retained its 68.4% of the initial activity.
27154518	6	48	theme	Optimum	754:760	arg1	temperature					762:772	Optimum temperature	754:772	Optimum temperature of the enzyme	754:786	Optimum temperature of the enzyme was shifted from 35°C to 45°C after immobilization.
27154518	1	49	theme	tea	271:273	arg1	samples					275:281	different tea samples	261:281	different tea samples	261:281	β-Glucosidase was covalently immobilized on chitosan-MWCNTs carrier and its aroma enhancement effect in different tea samples was investigated.
26580512	3	0	dep	nanocrystals	403:414	arg1	β-NaYF4					416:422	β-NaYF4	416:422	up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+)	389:436	To minimize its photobleaching, up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+) were prepared and modified with α-cyclodextrin, serving as excitation host.
26580512	0	1	link	rhodamine-derived	92:108	arg1	chemosensor					110:120	a rhodamine-derived chemosensor	90:120	a rhodamine-derived chemosensor	90:120	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26580512	3	2	theme	excitation	497:506	arg1	host					508:511	excitation host	497:511	excitation host	497:511	To minimize its photobleaching, up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+) were prepared and modified with α-cyclodextrin, serving as excitation host.
26580512	1	3	theme	cysteine	262:269	arg1	detection					271:279	cysteine detection	262:279	cysteine detection	262:279	In this paper, we reported an optical sensing composite for cysteine detection.
26580512	3	4	dep	β-NaYF4	416:422	arg1	3+					434:435	3+	434:435	3+	434:435	To minimize its photobleaching, up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+) were prepared and modified with α-cyclodextrin, serving as excitation host.
26580512	3	4	dep	β-NaYF4	416:422	arg1	/Er					430:432	Yb(3+)/Er	424:432	up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+)	389:436	To minimize its photobleaching, up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+) were prepared and modified with α-cyclodextrin, serving as excitation host.
26580512	8	5	theme	turn-on	1005:1011	arg1	effect					1014:1019	Emission "turn-on" effect	995:1019	Emission "turn-on" effect	995:1019	Emission "turn-on" effect was observed only for cysteine but immune to other competing amino acids and thiols, showing a good selectivity.
26580512	4	6	theme	nanocrystals	576:587	arg1	emission					544:551	emission	544:551	emission of these up-conversion nanocrystals	544:587	Under 980nm laser excitation, emission of these up-conversion nanocrystals overlapped well with the absorption of our chemosensor.
26580512	6	7	theme	simple	831:836	arg1	equilibrium					776:786	the complexation equilibrium	759:786	the complexation equilibrium between our chemosensor and cysteine	759:823	Job's analysis suggested that the complexation equilibrium between our chemosensor and cysteine was a simple one with binding stoichiometry of 1:1.
26580512	6	7	theme	simple	831:836	arg1	one					838:840	a simple one	829:840	a simple one with binding stoichiometry of 1:1	829:874	Job's analysis suggested that the complexation equilibrium between our chemosensor and cysteine was a simple one with binding stoichiometry of 1:1.
26580512	2	8	theme	rhodamine	309:317	arg1	6G					319:320	rhodamine 6G	309:320	rhodamine 6G	309:320	A chemosensor derived from rhodamine 6G was synthesized and characterized.
26580512	3	9	theme	up-conversion	389:401	arg1	nanocrystals					403:414	up-conversion nanocrystals	389:414	up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+)	389:436	To minimize its photobleaching, up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+) were prepared and modified with α-cyclodextrin, serving as excitation host.
26580512	8	10	theme	competing	1072:1080	arg1	acids					1088:1092	other competing amino acids	1066:1092	other competing amino acids	1066:1092	Emission "turn-on" effect was observed only for cysteine but immune to other competing amino acids and thiols, showing a good selectivity.
26580512	8	11	theme	"	1012:1012	arg1	effect					1014:1019	Emission "turn-on" effect	995:1019	Emission "turn-on" effect	995:1019	Emission "turn-on" effect was observed only for cysteine but immune to other competing amino acids and thiols, showing a good selectivity.
26580512	7	12	theme	sensing	879:885	arg1	system					887:892	A sensing system	877:892	A sensing system	877:892	A sensing system was constructed with up-conversion nanocrystals (modified with α-cyclodextrin) and this chemosensor.
26580512	8	13	theme	other	1066:1070	arg1	acids					1088:1092	other competing amino acids	1066:1092	other competing amino acids	1066:1092	Emission "turn-on" effect was observed only for cysteine but immune to other competing amino acids and thiols, showing a good selectivity.
26580512	0	14	theme	optical	3:9	arg1	composite					19:27	An optical sensing composite	0:27	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.	0:200	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26580512	5	15	theme	Energy	645:650	arg1	transfer					652:659	Energy transfer	645:659	Energy transfer between them	645:672	Energy transfer between them was analyzed and confirmed by emission decay analysis.
26580512	3	16	theme	Yb	424:425	arg1	3+					434:435	3+	434:435	3+	434:435	To minimize its photobleaching, up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+) were prepared and modified with α-cyclodextrin, serving as excitation host.
26580512	3	16	theme	Yb	424:425	arg1	/Er					430:432	Yb(3+)/Er	424:432	up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+)	389:436	To minimize its photobleaching, up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+) were prepared and modified with α-cyclodextrin, serving as excitation host.
26580512	5	17	theme	emission	704:711	arg1	analysis					719:726	emission decay analysis	704:726	emission decay analysis	704:726	Energy transfer between them was analyzed and confirmed by emission decay analysis.
26580512	6	18	with	one	838:840	arg1	stoichiometry					855:867	binding stoichiometry	847:867	binding stoichiometry of 1:1	847:874	Job's analysis suggested that the complexation equilibrium between our chemosensor and cysteine was a simple one with binding stoichiometry of 1:1.
26580512	5	19	theme	decay	713:717	arg1	analysis					719:726	emission decay analysis	704:726	emission decay analysis	704:726	Energy transfer between them was analyzed and confirmed by emission decay analysis.
26580512	0	20	theme	sensing	11:17	arg1	composite					19:27	An optical sensing composite	0:27	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.	0:200	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26580512	4	21	theme	up-conversion	562:574	arg1	nanocrystals					576:587	these up-conversion nanocrystals	556:587	these up-conversion nanocrystals	556:587	Under 980nm laser excitation, emission of these up-conversion nanocrystals overlapped well with the absorption of our chemosensor.
26580512	4	22	theme	chemosensor	632:642	arg1	absorption					614:623	the absorption	610:623	the absorption of our chemosensor	610:642	Under 980nm laser excitation, emission of these up-conversion nanocrystals overlapped well with the absorption of our chemosensor.
26580512	0	23	theme	cysteine	33:40	arg1	detection					42:50	cysteine detection	33:50	cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor	33:120	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26580512	0	24	theme	photophysical	155:167	arg1	feature					169:175	photophysical feature	155:175	photophysical feature	155:175	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26580512	4	25	theme	laser	526:530	arg1	excitation					532:541	980nm laser excitation	520:541	980nm laser excitation	520:541	Under 980nm laser excitation, emission of these up-conversion nanocrystals overlapped well with the absorption of our chemosensor.
26580512	4	26	theme	980nm	520:524	arg1	excitation					532:541	980nm laser excitation	520:541	980nm laser excitation	520:541	Under 980nm laser excitation, emission of these up-conversion nanocrystals overlapped well with the absorption of our chemosensor.
26580512	8	27	theme	good	1116:1119	arg1	selectivity					1121:1131	a good selectivity	1114:1131	a good selectivity	1114:1131	Emission "turn-on" effect was observed only for cysteine but immune to other competing amino acids and thiols, showing a good selectivity.
26580512	6	28	theme	1:1	872:874	arg1	stoichiometry					855:867	binding stoichiometry	847:867	binding stoichiometry of 1:1	847:874	Job's analysis suggested that the complexation equilibrium between our chemosensor and cysteine was a simple one with binding stoichiometry of 1:1.
26580512	6	29	theme	complexation	763:774	arg1	equilibrium					776:786	the complexation equilibrium	759:786	the complexation equilibrium between our chemosensor and cysteine	759:823	Job's analysis suggested that the complexation equilibrium between our chemosensor and cysteine was a simple one with binding stoichiometry of 1:1.
26580512	6	29	theme	complexation	763:774	arg1	one					838:840	a simple one	829:840	a simple one with binding stoichiometry of 1:1	829:874	Job's analysis suggested that the complexation equilibrium between our chemosensor and cysteine was a simple one with binding stoichiometry of 1:1.
26580512	2	30	attach	derived	296:302	arg2	chemosensor					284:294	A chemosensor	282:294	A chemosensor derived from rhodamine 6G	282:320	A chemosensor derived from rhodamine 6G was synthesized and characterized.
26580512	2	30	attach	derived	296:302	arg1	6G					319:320	rhodamine 6G	309:320	rhodamine 6G	309:320	A chemosensor derived from rhodamine 6G was synthesized and characterized.
26580512	0	31	dep	composite	19:27	arg1	performance					189:199	sensing performance	181:199	sensing performance	181:199	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26580512	0	31	dep	composite	19:27	arg1	characterization					137:152	characterization	137:152	characterization	137:152	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26580512	0	31	dep	composite	19:27	arg1	feature					169:175	photophysical feature	155:175	photophysical feature	155:175	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26580512	0	31	dep	composite	19:27	arg1	Construction					123:134	Construction	123:134	Construction	123:134	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26580512	7	32	dep	nanocrystals	929:940	arg1	modified					943:950	modified	943:950	modified with α-cyclodextrin	943:970	A sensing system was constructed with up-conversion nanocrystals (modified with α-cyclodextrin) and this chemosensor.
26580512	1	33	theme	optical	232:238	arg1	composite					248:256	an optical sensing composite	229:256	an optical sensing composite for cysteine detection	229:279	In this paper, we reported an optical sensing composite for cysteine detection.
26580512	0	34	theme	sensing	181:187	arg1	performance					189:199	sensing performance	181:199	sensing performance	181:199	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26580512	6	35	theme	binding	847:853	arg1	stoichiometry					855:867	binding stoichiometry	847:867	binding stoichiometry of 1:1	847:874	Job's analysis suggested that the complexation equilibrium between our chemosensor and cysteine was a simple one with binding stoichiometry of 1:1.
26580512	3	36	mod	modified	456:463	arg3	α-cyclodextrin					470:483	α-cyclodextrin	470:483	α-cyclodextrin	470:483	To minimize its photobleaching, up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+) were prepared and modified with α-cyclodextrin, serving as excitation host.
26580512	3	36	mod	modified	456:463	arg1	nanocrystals					403:414	up-conversion nanocrystals	389:414	up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+)	389:436	To minimize its photobleaching, up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+) were prepared and modified with α-cyclodextrin, serving as excitation host.
26580512	7	37	theme	up-conversion	915:927	arg1	nanocrystals					929:940	up-conversion nanocrystals	915:940	up-conversion nanocrystals (modified with α-cyclodextrin)	915:971	A sensing system was constructed with up-conversion nanocrystals (modified with α-cyclodextrin) and this chemosensor.
26580512	1	38	theme	sensing	240:246	arg1	composite					248:256	an optical sensing composite	229:256	an optical sensing composite for cysteine detection	229:279	In this paper, we reported an optical sensing composite for cysteine detection.
26580512	8	39	theme	amino	1082:1086	arg1	acids					1088:1092	other competing amino acids	1066:1092	other competing amino acids	1066:1092	Emission "turn-on" effect was observed only for cysteine but immune to other competing amino acids and thiols, showing a good selectivity.
26580512	0	40	theme	up-conversion	58:70	arg1	nanoparticles					72:84	up-conversion nanoparticles	58:84	up-conversion nanoparticles	58:84	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26580512	8	41	theme	Emission	995:1002	arg1	effect					1014:1019	Emission "turn-on" effect	995:1019	Emission "turn-on" effect	995:1019	Emission "turn-on" effect was observed only for cysteine but immune to other competing amino acids and thiols, showing a good selectivity.
26580512	0	42	theme	rhodamine-derived	92:108	arg1	chemosensor					110:120	a rhodamine-derived chemosensor	90:120	a rhodamine-derived chemosensor	90:120	An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
26919718	9	0	theme	inoculum	1799:1806	arg1	type					1791:1794	type	1791:1794	type	1791:1794	Under the limits of this study, the cariogenic potential of biofilms, formed under identical conditions in vitro, is similar, regardless of baseline differences between the source and type of inoculum.
26919718	9	0	theme	inoculum	1799:1806	arg1	source					1780:1785	source	1780:1785	source	1780:1785	Under the limits of this study, the cariogenic potential of biofilms, formed under identical conditions in vitro, is similar, regardless of baseline differences between the source and type of inoculum.
26919718	4	1	theme	variables	574:582	arg1	analysis					554:561	analysis	554:561	analysis of outcome variables	554:582	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	1	2	theme	caries-free	242:252	arg1	individuals					254:264	caries-active and caries-free individuals	224:264	caries-active and caries-free individuals	224:264	This study investigated the cariogenic potential of biofilms originating from different types of inoculum (saliva and dental plaque) from caries-active and caries-free individuals.
26919718	6	3	theme	acid-tolerant	1184:1196	arg1	microorganisms					1198:1211	acid-tolerant microorganisms	1184:1211	acid-tolerant microorganisms in saliva	1184:1221	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	4	4	theme	microbiological	634:648	arg1	composition					650:660	microbiological composition	634:660	microbiological composition of biofilms	634:672	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	8	5	theme	lactobacilli	1581:1592	arg1	lactobacilli					1581:1592	lactobacilli	1581:1592	lactobacilli (r = 0.379)	1581:1604	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	8	5	theme	lactobacilli	1581:1592	arg1	counts					1532:1537	CFU counts	1528:1537	CFU counts	1528:1537	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	8	5	theme	lactobacilli	1581:1592	arg1	bacteria					1556:1563	acid-tolerant bacteria	1542:1563	acid-tolerant bacteria (r = 0.406)	1542:1575	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	8	5	theme	lactobacilli	1581:1592	arg1	%					1519:1519	%SHC and CFU counts	1519:1537	%	1519:1519	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	6	6	theme	baseline	865:872	arg1	data					874:877	microbiological baseline data	849:877	microbiological baseline data	849:877	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	0	7	theme	in	57:58	arg1	Biofilms					76:83	in vitro Microcosm Biofilms	57:83	in vitro Microcosm Biofilms	57:83	Influence of the Inoculum Source on the Cariogenicity of in vitro Microcosm Biofilms.
26919718	2	8	theme	caries	306:311	arg1	condition					313:321	each caries condition	301:321	each caries condition	301:321	Ten volunteers were selected from each caries condition for the paired collection of saliva and dental plaque.
26919718	1	9	theme	inoculum	183:190	arg1	types					174:178	different types	164:178	different types of inoculum (saliva and dental plaque)	164:217	This study investigated the cariogenic potential of biofilms originating from different types of inoculum (saliva and dental plaque) from caries-active and caries-free individuals.
26919718	6	10	theme	dental	1163:1168	arg1	plaque					1170:1175	dental plaque	1163:1175	dental plaque	1163:1175	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	4	11	theme	%	624:624	arg1	SHC					625:627	%SHC	624:627	%SHC	624:627	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	4	11	theme	%	624:624	arg1	change					616:621	surface hardness change	599:621	surface hardness change (%SHC)	599:628	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	6	12	theme	caries-active	948:960	arg1	individuals					962:972	caries-active individuals	948:972	caries-active individuals	948:972	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	6	13	theme	different	1059:1067	arg1	status					1076:1081	different caries status	1059:1081	different caries status	1059:1081	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	1	14	theme	dental	204:209	arg1	plaque					211:216	dental plaque	204:216	dental plaque	204:216	This study investigated the cariogenic potential of biofilms originating from different types of inoculum (saliva and dental plaque) from caries-active and caries-free individuals.
26919718	9	15	theme	cariogenic	1643:1652	arg1	potential					1654:1662	the cariogenic potential	1639:1662	the cariogenic potential	1639:1662	Under the limits of this study, the cariogenic potential of biofilms, formed under identical conditions in vitro, is similar, regardless of baseline differences between the source and type of inoculum.
26919718	9	15	theme	cariogenic	1643:1652	arg1	similar					1724:1730	similar	1724:1730	similar	1724:1730	Under the limits of this study, the cariogenic potential of biofilms, formed under identical conditions in vitro, is similar, regardless of baseline differences between the source and type of inoculum.
26919718	6	16	theme	acid-tolerant	1107:1119	arg1	microorganisms					1121:1134	acid-tolerant microorganisms	1107:1134	acid-tolerant microorganisms	1107:1134	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	7	17	theme	growth	1249:1254	arg1	days					1233:1236	10 days	1230:1236	10 days of biofilm growth	1230:1254	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	1	18	theme	biofilms	138:145	arg1	potential					125:133	the cariogenic potential	110:133	the cariogenic potential of biofilms originating from different types of inoculum (saliva and dental plaque) from caries-active and caries-free individuals	110:264	This study investigated the cariogenic potential of biofilms originating from different types of inoculum (saliva and dental plaque) from caries-active and caries-free individuals.
26919718	6	19	theme	unit	1016:1019	arg1	counts					1027:1032	colony-forming unit (CFU) counts	1001:1032	colony-forming unit (CFU) counts between individuals with different caries status	1001:1081	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	6	20	from	saliva	1216:1221	arg1	counts					1097:1102	higher counts	1090:1102	higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva	1090:1221	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	8	21	dep	lactobacilli	1581:1592	arg1	=					1597:1597	=	1597:1597	=	1597:1597	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	8	22	theme	positive	1484:1491	arg1	correlation					1493:1503	A positive correlation	1482:1503	A positive correlation	1482:1503	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	0	23	theme	Biofilms	76:83	arg1	Cariogenicity					40:52	the Cariogenicity	36:52	the Cariogenicity of in vitro Microcosm Biofilms	36:83	Influence of the Inoculum Source on the Cariogenicity of in vitro Microcosm Biofilms.
26919718	6	24	theme	microorganisms	1198:1211	arg1	counts					1097:1102	higher counts	1090:1102	higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva	1090:1221	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	6	25	theme	comparative	977:987	arg1	analysis					989:996	a comparative analysis	975:996	a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status	975:1081	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	3	26	theme	Microcosm	378:386	arg1	biofilms					388:395	Microcosm biofilms	378:395	Microcosm biofilms	378:395	Microcosm biofilms were grown in triplicate from each inoculum on enamel specimens in 24-well plates under cariogenic challenge.
26919718	7	27	theme	biofilm	1241:1247	arg1	growth					1249:1254	biofilm growth	1241:1254	biofilm growth	1241:1254	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	6	28	from	microorganisms	1198:1211	arg1	saliva					1216:1221	saliva	1216:1221	saliva	1216:1221	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	7	29	theme	acid-tolerant	1336:1348	arg1	bacteria					1350:1357	acid-tolerant bacteria	1336:1357	acid-tolerant bacteria	1336:1357	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	5	30	theme	=	809:809	arg1	α					807:807	α = 0.05	807:814	α = 0.05	807:814	Statistical analysis was performed using the t test, the linear multivariate analysis model and Pearson's correlation coefficients (α = 0.05).
26919718	5	30	theme	=	809:809	arg1	coefficients					793:804	Pearson's correlation coefficients	771:804	Pearson's correlation coefficients (α = 0.05)	771:815	Statistical analysis was performed using the t test, the linear multivariate analysis model and Pearson's correlation coefficients (α = 0.05).
26919718	6	31	theme	microbiological	849:863	arg1	data					874:877	microbiological baseline data	849:877	microbiological baseline data	849:877	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	8	32	theme	CFU	1528:1530	arg1	counts					1532:1537	CFU counts	1528:1537	CFU counts	1528:1537	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	3	33	theme	enamel	444:449	arg1	specimens					451:459	enamel specimens	444:459	enamel specimens	444:459	Microcosm biofilms were grown in triplicate from each inoculum on enamel specimens in 24-well plates under cariogenic challenge.
26919718	0	34	theme	Source	26:31	arg1	Influence					0:8	Influence	0:8	Influence of the Inoculum Source on the Cariogenicity of in vitro Microcosm Biofilms.	0:84	Influence of the Inoculum Source on the Cariogenicity of in vitro Microcosm Biofilms.
26919718	6	35	theme	counts	1027:1032	arg1	analysis					989:996	a comparative analysis	975:996	a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status	975:1081	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	5	36	theme	multivariate	739:750	arg1	analysis					752:759	the linear multivariate analysis	728:759	the linear multivariate analysis model	728:765	Statistical analysis was performed using the t test, the linear multivariate analysis model and Pearson's correlation coefficients (α = 0.05).
26919718	3	37	theme	cariogenic	485:494	arg1	challenge					496:504	cariogenic challenge	485:504	cariogenic challenge	485:504	Microcosm biofilms were grown in triplicate from each inoculum on enamel specimens in 24-well plates under cariogenic challenge.
26919718	5	38	theme	correlation	781:791	arg1	α					807:807	α = 0.05	807:814	α = 0.05	807:814	Statistical analysis was performed using the t test, the linear multivariate analysis model and Pearson's correlation coefficients (α = 0.05).
26919718	5	38	theme	correlation	781:791	arg1	coefficients					793:804	Pearson's correlation coefficients	771:804	Pearson's correlation coefficients (α = 0.05)	771:815	Statistical analysis was performed using the t test, the linear multivariate analysis model and Pearson's correlation coefficients (α = 0.05).
26919718	6	39	theme	mutans	1140:1145	arg1	streptococci					1147:1158	mutans streptococci	1140:1158	mutans streptococci	1140:1158	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	8	40	theme	bacteria	1556:1563	arg1	lactobacilli					1581:1592	lactobacilli	1581:1592	lactobacilli (r = 0.379)	1581:1604	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	8	40	theme	bacteria	1556:1563	arg1	counts					1532:1537	CFU counts	1528:1537	CFU counts	1528:1537	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	8	40	theme	bacteria	1556:1563	arg1	bacteria					1556:1563	acid-tolerant bacteria	1542:1563	acid-tolerant bacteria (r = 0.406)	1542:1575	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	8	40	theme	bacteria	1556:1563	arg1	%					1519:1519	%SHC and CFU counts	1519:1537	%	1519:1519	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	8	41	dep	%	1519:1519	arg1	SHC					1520:1522	SHC	1520:1522	SHC	1520:1522	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	7	42	theme	total	1276:1280	arg1	microorganisms					1282:1295	total microorganisms	1276:1295	total microorganisms	1276:1295	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	2	43	theme	dental	363:368	arg1	plaque					370:375	dental plaque	363:375	dental plaque	363:375	Ten volunteers were selected from each caries condition for the paired collection of saliva and dental plaque.
26919718	6	44	theme	streptococci	910:921	arg1	counts					893:898	higher counts	886:898	higher counts of mutans streptococci in plaque samples within caries-active individuals	886:972	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	4	45	theme	hardness	607:614	arg1	SHC					625:627	%SHC	624:627	%SHC	624:627	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	4	45	theme	hardness	607:614	arg1	change					616:621	surface hardness change	599:621	surface hardness change (%SHC)	599:628	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	5	46	theme	analysis	752:759	arg1	model					761:765	the linear multivariate analysis model	728:765	the linear multivariate analysis model	728:765	Statistical analysis was performed using the t test, the linear multivariate analysis model and Pearson's correlation coefficients (α = 0.05).
26919718	7	47	theme	caries	1453:1458	arg1	condition					1460:1468	caries condition	1453:1468	caries condition (p > 0.05)	1453:1479	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	7	47	theme	caries	1453:1458	arg1	>					1473:1473	p > 0.05	1471:1478	p > 0.05	1471:1478	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	2	48	theme	saliva	352:357	arg1	collection					338:347	the paired collection	327:347	the paired collection of saliva and dental plaque	327:375	Ten volunteers were selected from each caries condition for the paired collection of saliva and dental plaque.
26919718	7	49	theme	p	1471:1471	arg1	condition					1460:1468	caries condition	1453:1468	caries condition (p > 0.05)	1453:1479	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	7	49	theme	p	1471:1471	arg1	>					1473:1473	p > 0.05	1471:1478	p > 0.05	1471:1478	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	6	50	theme	higher	1090:1095	arg1	counts					1097:1102	higher counts	1090:1102	higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva	1090:1221	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	4	51	theme	outcome	566:572	arg1	variables					574:582	outcome variables	566:582	outcome variables	566:582	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	6	52	theme	higher	886:891	arg1	counts					893:898	higher counts	886:898	higher counts of mutans streptococci in plaque samples within caries-active individuals	886:972	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	7	53	theme	condition	1460:1468	arg1	type					1432:1435	the type	1428:1435	the type of inoculum and caries condition (p > 0.05)	1428:1479	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	7	54	theme	inoculum	1440:1447	arg1	type					1432:1435	the type	1428:1435	the type of inoculum and caries condition (p > 0.05)	1428:1479	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	1	55	theme	different	164:172	arg1	types					174:178	different types	164:178	different types of inoculum (saliva and dental plaque)	164:217	This study investigated the cariogenic potential of biofilms originating from different types of inoculum (saliva and dental plaque) from caries-active and caries-free individuals.
26919718	9	56	theme	study	1632:1636	arg1	limits					1617:1622	the limits	1613:1622	the limits of this study	1613:1636	Under the limits of this study, the cariogenic potential of biofilms, formed under identical conditions in vitro, is similar, regardless of baseline differences between the source and type of inoculum.
26919718	8	57	dep	bacteria	1556:1563	arg1	=					1568:1568	=	1568:1568	=	1568:1568	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	0	58	from	Influence	0:8	arg1	Cariogenicity					40:52	the Cariogenicity	36:52	the Cariogenicity of in vitro Microcosm Biofilms	36:83	Influence of the Inoculum Source on the Cariogenicity of in vitro Microcosm Biofilms.
26919718	6	59	theme	plaque	926:931	arg1	samples					933:939	plaque samples	926:939	plaque samples within caries-active individuals	926:972	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	5	60	theme	Statistical	675:685	arg1	analysis					687:694	Statistical analysis	675:694	Statistical analysis	675:694	Statistical analysis was performed using the t test, the linear multivariate analysis model and Pearson's correlation coefficients (α = 0.05).
26919718	1	61	theme	cariogenic	114:123	arg1	potential					125:133	the cariogenic potential	110:133	the cariogenic potential of biofilms originating from different types of inoculum (saliva and dental plaque) from caries-active and caries-free individuals	110:264	This study investigated the cariogenic potential of biofilms originating from different types of inoculum (saliva and dental plaque) from caries-active and caries-free individuals.
26919718	9	62	theme	biofilms	1667:1674	arg1	potential					1654:1662	the cariogenic potential	1639:1662	the cariogenic potential	1639:1662	Under the limits of this study, the cariogenic potential of biofilms, formed under identical conditions in vitro, is similar, regardless of baseline differences between the source and type of inoculum.
26919718	9	62	theme	biofilms	1667:1674	arg1	similar					1724:1730	similar	1724:1730	similar	1724:1730	Under the limits of this study, the cariogenic potential of biofilms, formed under identical conditions in vitro, is similar, regardless of baseline differences between the source and type of inoculum.
26919718	5	63	theme	linear	732:737	arg1	analysis					752:759	the linear multivariate analysis	728:759	the linear multivariate analysis model	728:765	Statistical analysis was performed using the t test, the linear multivariate analysis model and Pearson's correlation coefficients (α = 0.05).
26919718	4	64	theme	biofilms	665:672	arg1	percentage					585:594	percentage	585:594	percentage of surface hardness change (%SHC)	585:628	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	4	64	theme	biofilms	665:672	arg1	composition					650:660	microbiological composition	634:660	microbiological composition of biofilms	634:672	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	0	65	theme	Microcosm	66:74	arg1	Biofilms					76:83	in vitro Microcosm Biofilms	57:83	in vitro Microcosm Biofilms	57:83	Influence of the Inoculum Source on the Cariogenicity of in vitro Microcosm Biofilms.
26919718	6	66	theme	colony-forming	1001:1014	arg1	CFU					1022:1024	CFU	1022:1024	CFU	1022:1024	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	6	66	theme	colony-forming	1001:1014	arg1	unit					1016:1019	colony-forming unit	1001:1019	colony-forming unit (CFU) counts between individuals with different caries status	1001:1081	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	9	67	theme	identical	1690:1698	arg1	conditions					1700:1709	identical conditions	1690:1709	identical conditions	1690:1709	Under the limits of this study, the cariogenic potential of biofilms, formed under identical conditions in vitro, is similar, regardless of baseline differences between the source and type of inoculum.
26919718	5	68	theme	t	720:720	arg1	test					722:725	the t test	716:725	the t test	716:725	Statistical analysis was performed using the t test, the linear multivariate analysis model and Pearson's correlation coefficients (α = 0.05).
26919718	6	69	theme	microorganisms	1121:1134	arg1	counts					1097:1102	higher counts	1090:1102	higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva	1090:1221	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	9	70	dep	source	1780:1785	arg1	the					1776:1778	the	1776:1778	the	1776:1778	Under the limits of this study, the cariogenic potential of biofilms, formed under identical conditions in vitro, is similar, regardless of baseline differences between the source and type of inoculum.
26919718	4	71	dep	collected	540:548	arg1	percentage					585:594	percentage	585:594	percentage of surface hardness change (%SHC)	585:628	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	4	71	dep	collected	540:548	arg1	composition					650:660	microbiological composition	634:660	microbiological composition of biofilms	634:672	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	6	72	theme	caries	1069:1074	arg1	status					1076:1081	different caries status	1059:1081	different caries status	1059:1081	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	2	73	theme	plaque	370:375	arg1	collection					338:347	the paired collection	327:347	the paired collection of saliva and dental plaque	327:375	Ten volunteers were selected from each caries condition for the paired collection of saliva and dental plaque.
26919718	0	74	theme	Inoculum	17:24	arg1	Source					26:31	the Inoculum Source	13:31	the Inoculum Source	13:31	Influence of the Inoculum Source on the Cariogenicity of in vitro Microcosm Biofilms.
26919718	0	75	dep	in	57:58	arg1	vitro					60:64	vitro	60:64	vitro	60:64	Influence of the Inoculum Source on the Cariogenicity of in vitro Microcosm Biofilms.
26919718	3	76	theme	24-well	464:470	arg1	plates					472:477	24-well plates	464:477	24-well plates	464:477	Microcosm biofilms were grown in triplicate from each inoculum on enamel specimens in 24-well plates under cariogenic challenge.
26919718	9	77	theme	baseline	1747:1754	arg1	differences					1756:1766	baseline differences	1747:1766	baseline differences between the source and type of inoculum	1747:1806	Under the limits of this study, the cariogenic potential of biofilms, formed under identical conditions in vitro, is similar, regardless of baseline differences between the source and type of inoculum.
26919718	8	78	theme	acid-tolerant	1542:1554	arg1	bacteria					1556:1563	acid-tolerant bacteria	1542:1563	acid-tolerant bacteria (r = 0.406)	1542:1575	A positive correlation was found for %SHC and CFU counts of acid-tolerant bacteria (r = 0.406) and lactobacilli (r = 0.379).
26919718	1	79	dep	inoculum	183:190	arg1	saliva					193:198	saliva	193:198	saliva	193:198	This study investigated the cariogenic potential of biofilms originating from different types of inoculum (saliva and dental plaque) from caries-active and caries-free individuals.
26919718	1	79	dep	inoculum	183:190	arg1	plaque					211:216	dental plaque	204:216	dental plaque	204:216	This study investigated the cariogenic potential of biofilms originating from different types of inoculum (saliva and dental plaque) from caries-active and caries-free individuals.
26919718	6	80	theme	comparative	820:830	arg1	analysis					832:839	A comparative analysis	818:839	A comparative analysis between microbiological baseline data	818:877	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	6	81	theme	streptococci	1147:1158	arg1	counts					1097:1102	higher counts	1090:1102	higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva	1090:1221	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	2	82	theme	paired	331:336	arg1	collection					338:347	the paired collection	327:347	the paired collection of saliva and dental plaque	327:375	Ten volunteers were selected from each caries condition for the paired collection of saliva and dental plaque.
26919718	4	83	theme	surface	599:605	arg1	SHC					625:627	%SHC	624:627	%SHC	624:627	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	4	83	theme	surface	599:605	arg1	change					616:621	surface hardness change	599:621	surface hardness change (%SHC)	599:628	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	6	84	from	counts	1097:1102	arg1	plaque					1170:1175	dental plaque	1163:1175	dental plaque	1163:1175	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	6	84	from	counts	1097:1102	arg1	saliva					1216:1221	saliva	1216:1221	saliva	1216:1221	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	6	85	with	individuals	1042:1052	arg1	status					1076:1081	different caries status	1059:1081	different caries status	1059:1081	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	7	86	theme	CFU	1261:1263	arg1	values					1265:1270	the CFU values	1257:1270	the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC,	1257:1378	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	7	86	theme	CFU	1261:1263	arg1	significant					1403:1413	significant	1403:1413	significant	1403:1413	After 10 days of biofilm growth, the CFU values for total microorganisms, lactobacilli, mutans streptococci and acid-tolerant bacteria, as well as for SHC, were not statistically significant, considering the type of inoculum and caries condition (p > 0.05).
26919718	4	87	theme	change	616:621	arg1	percentage					585:594	percentage	585:594	percentage of surface hardness change (%SHC)	585:628	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	4	87	theme	change	616:621	arg1	composition					650:660	microbiological composition	634:660	microbiological composition of biofilms	634:672	After 10 days, the biofilms were collected for analysis of outcome variables: percentage of surface hardness change (%SHC) and microbiological composition of biofilms.
26919718	6	88	from	counts	893:898	arg1	samples					933:939	plaque samples	926:939	plaque samples within caries-active individuals	926:972	A comparative analysis between microbiological baseline data showed higher counts of mutans streptococci in plaque samples within caries-active individuals; a comparative analysis of colony-forming unit (CFU) counts between individuals with different caries status showed higher counts of acid-tolerant microorganisms and mutans streptococci in dental plaque and of acid-tolerant microorganisms in saliva.
26919718	1	89	theme	caries-active	224:236	arg1	individuals					254:264	caries-active and caries-free individuals	224:264	caries-active and caries-free individuals	224:264	This study investigated the cariogenic potential of biofilms originating from different types of inoculum (saliva and dental plaque) from caries-active and caries-free individuals.
26938809	5	0	theme	products	795:802	arg1	composition					804:814	the pyrolysis products composition	781:814	the pyrolysis products composition of T. weissflogii	781:832	The results showed that the catalysts had a significant effect on the pyrolysis products composition of T. weissflogii.
26938809	6	1	theme	wt	953:954	arg1	algae					964:968	the algae	960:968	the algae starting O2 content	960:988	CeO2, NiCeAl2O3 and MgCe/Al2O3 catalysts increased the aliphatics and decreased the oxygen content in bio-oils to 6-7 wt% of the algae starting O2 content.
26938809	6	1	theme	wt	953:954	arg1	%					955:955	6-7 wt%	949:955	6-7 wt% of the algae starting O2 content	949:988	CeO2, NiCeAl2O3 and MgCe/Al2O3 catalysts increased the aliphatics and decreased the oxygen content in bio-oils to 6-7 wt% of the algae starting O2 content.
26938809	1	2	theme	egg	175:177	arg1	powder					185:190	egg white powder	175:190	egg white powder	175:190	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	1	2	theme	egg	175:177	arg1	cellulose					164:172	microcrystalline cellulose	147:172	microcrystalline cellulose	147:172	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	0	3	theme	model	86:90	arg1	compounds					92:100	its model compounds	82:100	its model compounds	82:100	Ceria promoted deoxygenation and denitrogenation of Thalassiosira weissflogii and its model compounds by catalytic in-situ pyrolysis.
26938809	7	4	from	material	1106:1113	arg1	that					1087:1090	that	1087:1090	that	1087:1090	Ceria catalysts were also able to consistently reduce the N-content in the bio-oil to 20-38% of that in the parent material, with NiCe/Al2O3 being the most effective.
26938809	7	4	from	material	1106:1113	arg1	%					1082:1082	20-38%	1077:1082	20-38% of that in the parent material	1077:1113	Ceria catalysts were also able to consistently reduce the N-content in the bio-oil to 20-38% of that in the parent material, with NiCe/Al2O3 being the most effective.
26938809	1	5	theme	white	179:183	arg1	powder					185:190	egg white powder	175:190	egg white powder	175:190	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	1	5	theme	white	179:183	arg1	cellulose					164:172	microcrystalline cellulose	147:172	microcrystalline cellulose	147:172	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	0	6	theme	catalytic	105:113	arg1	pyrolysis					123:131	catalytic in-situ pyrolysis	105:131	catalytic in-situ pyrolysis	105:131	Ceria promoted deoxygenation and denitrogenation of Thalassiosira weissflogii and its model compounds by catalytic in-situ pyrolysis.
26938809	7	7	theme	Ceria	991:995	arg1	catalysts					997:1005	Ceria catalysts	991:1005	Ceria catalysts	991:1005	Ceria catalysts were also able to consistently reduce the N-content in the bio-oil to 20-38% of that in the parent material, with NiCe/Al2O3 being the most effective.
26938809	4	8	theme	decarbonylation	625:639	arg1	reactions					641:649	decarbonylation reactions	625:649	decarbonylation reactions	625:649	CeO2 increased decarbonylation reactions compared to in absence of catalyst, with production of ketones.
26938809	5	9	theme	significant	759:769	arg1	effect					771:776	a significant effect	757:776	a significant effect	757:776	The results showed that the catalysts had a significant effect on the pyrolysis products composition of T. weissflogii.
26938809	3	10	theme	model	536:540	arg1	compounds					542:550	model compounds	536:550	model compounds	536:550	Dehydration and decarboxylation were the main reactions involved in the algae and model compounds deoxygenation, while nitrogen was removed as NH3 and HCN.
26938809	3	11	dep	algae	526:530	arg1	deoxygenation					552:564	deoxygenation	552:564	deoxygenation	552:564	Dehydration and decarboxylation were the main reactions involved in the algae and model compounds deoxygenation, while nitrogen was removed as NH3 and HCN.
26938809	3	11	dep	algae	526:530	arg1	the					522:524	the	522:524	the	522:524	Dehydration and decarboxylation were the main reactions involved in the algae and model compounds deoxygenation, while nitrogen was removed as NH3 and HCN.
26938809	1	12	theme	palm-jojoba	193:203	arg1	cellulose					164:172	microcrystalline cellulose	147:172	microcrystalline cellulose	147:172	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	1	12	theme	palm-jojoba	193:203	arg1	mixtures					210:217	palm-jojoba oils mixtures	193:217	palm-jojoba oils mixtures Thalassiosira weissflogii	193:243	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	0	13	theme	in-situ	115:121	arg1	pyrolysis					123:131	catalytic in-situ pyrolysis	105:131	catalytic in-situ pyrolysis	105:131	Ceria promoted deoxygenation and denitrogenation of Thalassiosira weissflogii and its model compounds by catalytic in-situ pyrolysis.
26938809	7	14	theme	parent	1099:1104	arg1	material					1106:1113	the parent material	1095:1113	the parent material	1095:1113	Ceria catalysts were also able to consistently reduce the N-content in the bio-oil to 20-38% of that in the parent material, with NiCe/Al2O3 being the most effective.
26938809	6	15	theme	oxygen	919:924	arg1	content					926:932	the oxygen content	915:932	the oxygen content in bio-oils	915:944	CeO2, NiCeAl2O3 and MgCe/Al2O3 catalysts increased the aliphatics and decreased the oxygen content in bio-oils to 6-7 wt% of the algae starting O2 content.
26938809	7	16	theme	that	1087:1090	arg1	that					1087:1090	that	1087:1090	that	1087:1090	Ceria catalysts were also able to consistently reduce the N-content in the bio-oil to 20-38% of that in the parent material, with NiCe/Al2O3 being the most effective.
26938809	7	16	theme	that	1087:1090	arg1	%					1082:1082	20-38%	1077:1082	20-38% of that in the parent material	1077:1113	Ceria catalysts were also able to consistently reduce the N-content in the bio-oil to 20-38% of that in the parent material, with NiCe/Al2O3 being the most effective.
26938809	2	17	theme	Light	342:346	arg1	organics					348:355	Light organics	342:355	Light organics	342:355	Light organics, aromatics and aliphatics were originated from carbohydrates, proteins and lipids, respectively.
26938809	4	18	theme	ketones	706:712	arg1	production					692:701	production	692:701	production of ketones	692:712	CeO2 increased decarbonylation reactions compared to in absence of catalyst, with production of ketones.
26938809	5	19	contain	had	753:755	arg1	catalysts					743:751	the catalysts	739:751	the catalysts	739:751	The results showed that the catalysts had a significant effect on the pyrolysis products composition of T. weissflogii.
26938809	5	19	contain	had	753:755	arg2	effect					771:776	a significant effect	757:776	a significant effect	757:776	The results showed that the catalysts had a significant effect on the pyrolysis products composition of T. weissflogii.
26938809	6	20	theme	MgCe/Al2O3	855:864	arg1	catalysts					866:874	MgCe/Al2O3 catalysts	855:874	MgCe/Al2O3 catalysts	855:874	CeO2, NiCeAl2O3 and MgCe/Al2O3 catalysts increased the aliphatics and decreased the oxygen content in bio-oils to 6-7 wt% of the algae starting O2 content.
26938809	1	21	theme	oils	205:208	arg1	cellulose					164:172	microcrystalline cellulose	147:172	microcrystalline cellulose	147:172	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	1	21	theme	oils	205:208	arg1	mixtures					210:217	palm-jojoba oils mixtures	193:217	palm-jojoba oils mixtures Thalassiosira weissflogii	193:243	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	1	22	from	500°C	288:292	arg1	CeO2					280:283	CeO2	280:283	CeO2 at 500°C	280:292	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	1	23	dep	cellulose	164:172	arg1	compounds					251:259	model compounds	245:259	model compounds	245:259	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	1	24	theme	catalytic	311:319	arg1	mechanism					331:339	its catalytic upgrading mechanism	307:339	its catalytic upgrading mechanism	307:339	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	4	25	theme	catalyst	677:684	arg1	absence					666:672	absence	666:672	absence of catalyst	666:684	CeO2 increased decarbonylation reactions compared to in absence of catalyst, with production of ketones.
26938809	5	26	theme	T.	819:820	arg1	composition					804:814	the pyrolysis products composition	781:814	the pyrolysis products composition of T. weissflogii	781:832	The results showed that the catalysts had a significant effect on the pyrolysis products composition of T. weissflogii.
26938809	1	27	theme	upgrading	321:329	arg1	mechanism					331:339	its catalytic upgrading mechanism	307:339	its catalytic upgrading mechanism	307:339	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	7	28	from	%	1082:1082	arg1	material					1106:1113	the parent material	1095:1113	the parent material	1095:1113	Ceria catalysts were also able to consistently reduce the N-content in the bio-oil to 20-38% of that in the parent material, with NiCe/Al2O3 being the most effective.
26938809	1	29	theme	model	245:249	arg1	compounds					251:259	model compounds	245:259	model compounds	245:259	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	6	30	theme	algae	964:968	arg1	algae					964:968	the algae	960:968	the algae starting O2 content	960:988	CeO2, NiCeAl2O3 and MgCe/Al2O3 catalysts increased the aliphatics and decreased the oxygen content in bio-oils to 6-7 wt% of the algae starting O2 content.
26938809	6	30	theme	algae	964:968	arg1	%					955:955	6-7 wt%	949:955	6-7 wt% of the algae starting O2 content	949:988	CeO2, NiCeAl2O3 and MgCe/Al2O3 catalysts increased the aliphatics and decreased the oxygen content in bio-oils to 6-7 wt% of the algae starting O2 content.
26938809	0	31	theme	Thalassiosira	52:64	arg1	deoxygenation					15:27	deoxygenation	15:27	deoxygenation	15:27	Ceria promoted deoxygenation and denitrogenation of Thalassiosira weissflogii and its model compounds by catalytic in-situ pyrolysis.
26938809	0	31	theme	Thalassiosira	52:64	arg1	compounds					92:100	its model compounds	82:100	its model compounds	82:100	Ceria promoted deoxygenation and denitrogenation of Thalassiosira weissflogii and its model compounds by catalytic in-situ pyrolysis.
26938809	0	31	theme	Thalassiosira	52:64	arg1	denitrogenation					33:47	denitrogenation	33:47	denitrogenation	33:47	Ceria promoted deoxygenation and denitrogenation of Thalassiosira weissflogii and its model compounds by catalytic in-situ pyrolysis.
26938809	7	32	from	N-content	1049:1057	arg1	bio-oil					1066:1072	the bio-oil	1062:1072	the bio-oil	1062:1072	Ceria catalysts were also able to consistently reduce the N-content in the bio-oil to 20-38% of that in the parent material, with NiCe/Al2O3 being the most effective.
26938809	6	33	from	content	926:932	arg1	bio-oils					937:944	bio-oils	937:944	bio-oils	937:944	CeO2, NiCeAl2O3 and MgCe/Al2O3 catalysts increased the aliphatics and decreased the oxygen content in bio-oils to 6-7 wt% of the algae starting O2 content.
26938809	1	34	theme	microcrystalline	147:162	arg1	powder					185:190	egg white powder	175:190	egg white powder	175:190	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	1	34	theme	microcrystalline	147:162	arg1	mixtures					210:217	palm-jojoba oils mixtures	193:217	palm-jojoba oils mixtures Thalassiosira weissflogii	193:243	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	1	34	theme	microcrystalline	147:162	arg1	cellulose					164:172	microcrystalline cellulose	147:172	microcrystalline cellulose	147:172	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	3	35	theme	main	495:498	arg1	decarboxylation					470:484	decarboxylation	470:484	decarboxylation	470:484	Dehydration and decarboxylation were the main reactions involved in the algae and model compounds deoxygenation, while nitrogen was removed as NH3 and HCN.
26938809	3	35	theme	main	495:498	arg1	Dehydration					454:464	Dehydration	454:464	Dehydration	454:464	Dehydration and decarboxylation were the main reactions involved in the algae and model compounds deoxygenation, while nitrogen was removed as NH3 and HCN.
26938809	3	35	theme	main	495:498	arg1	reactions					500:508	the main reactions	491:508	the main reactions involved in the algae and model compounds deoxygenation	491:564	Dehydration and decarboxylation were the main reactions involved in the algae and model compounds deoxygenation, while nitrogen was removed as NH3 and HCN.
26938809	1	36	dep	mixtures	210:217	arg1	Thalassiosira					219:231	Thalassiosira	219:231	Thalassiosira	219:231	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	1	37	theme	cellulose	164:172	arg1	Pyrolysis					134:142	Pyrolysis	134:142	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds	134:259	Pyrolysis of microcrystalline cellulose, egg white powder, palm-jojoba oils mixtures Thalassiosira weissflogii model compounds was performed with CeO2 at 500°C, to evaluate its catalytic upgrading mechanism.
26938809	5	38	theme	pyrolysis	785:793	arg1	products					795:802	pyrolysis products	785:802	the pyrolysis products composition of T. weissflogii	781:832	The results showed that the catalysts had a significant effect on the pyrolysis products composition of T. weissflogii.
27603917	6	0	from	starch	887:892	arg1	rich					860:863	rich	860:863	rich	860:863	All legume pasta were rich in protein, resistant starch and fibers.
27603917	2	1	theme	legume	325:330	arg1	flours					332:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	1	theme	legume	325:330	arg1	alternative					402:412	a good alternative	395:412	a good alternative for gluten-free pasta production	395:445	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	1	theme	legume	325:330	arg1	minerals					377:384	minerals	377:384	minerals	377:384	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	1	theme	legume	325:330	arg1	starch					366:371	resistant starch	356:371	resistant starch	356:371	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	1	2	theme	low	219:221	arg1	index					232:236	a low glycemic index	217:236	a low glycemic index	217:236	Wheat pasta has a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index, but is nevertheless unsuitable for gluten-intolerant people.
27603917	12	3	from	factors	1606:1612	arg1	α-galactosides					1666:1679	α-galactosides	1666:1679	α-galactosides	1666:1679	Anti-nutritional factors in legumes, such as trypsin inhibitory activity and α-galactosides were reduced by up to 82% and 73%, respectively, by pasta processing and cooking.
27603917	12	3	from	factors	1606:1612	arg1	legumes					1617:1623	legumes	1617:1623	legumes	1617:1623	Anti-nutritional factors in legumes, such as trypsin inhibitory activity and α-galactosides were reduced by up to 82% and 73%, respectively, by pasta processing and cooking.
27603917	12	3	from	factors	1606:1612	arg1	activity					1653:1660	trypsin inhibitory activity	1634:1660	trypsin inhibitory activity	1634:1660	Anti-nutritional factors in legumes, such as trypsin inhibitory activity and α-galactosides were reduced by up to 82% and 73%, respectively, by pasta processing and cooking.
27603917	10	4	theme	cereal	1274:1279	arg1	pasta					1293:1297	a commercial cereal gluten-free pasta	1261:1297	a commercial cereal gluten-free pasta	1261:1297	In comparison to a commercial cereal gluten-free pasta, all the legume pasta lost less material during cooking but was less cohesive and springy.
27603917	4	5	theme	in-vitro	639:646	arg1	digestion					655:663	their in-vitro starch digestion	633:663	their in-vitro starch digestion	633:663	The relationship between their structure, their cooking and Rheological properties and their in-vitro starch digestion was analyzed and compared to cereal gluten-free commercial pasta.
27603917	9	6	theme	lentil	1162:1167	arg1	pasta					1169:1173	lentil pasta	1162:1173	lentil pasta	1162:1173	Black-gram pasta, which is especially rich in soluble fibers, differed from faba and lentil pasta, with high springiness (0.85 vs. 0.75) and less loss during cooking.
27603917	3	7	theme	black-gram	527:536	arg1	flours					538:543	black-gram flours	527:543	black-gram flours	527:543	In this study, gluten-free pasta was produced exclusively from faba, lentil or black-gram flours.
27603917	0	8	theme	%	99:99	arg1	Pasta					108:112	100% Legume Pasta	96:112	100% Legume Pasta	96:112	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	3	9	theme	gluten-free	463:473	arg1	pasta					475:479	gluten-free pasta	463:479	gluten-free pasta	463:479	In this study, gluten-free pasta was produced exclusively from faba, lentil or black-gram flours.
27603917	1	10	theme	gluten	162:167	arg1	network					169:175	a gluten network	160:175	a gluten network entrapping starch granules resulting in a low glycemic index	160:236	Wheat pasta has a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index, but is nevertheless unsuitable for gluten-intolerant people.
27603917	14	11	with	pasta	1920:1924	arg1	quality					1973:1979	high nutritional quality	1956:1979	high nutritional quality	1956:1979	This study demonstrates the advantages of using legumes for the production of gluten-free pasta with a low glycemic index and high nutritional quality.
27603917	14	11	with	pasta	1920:1924	arg1	index					1946:1950	a low glycemic index	1931:1950	a low glycemic index	1931:1950	This study demonstrates the advantages of using legumes for the production of gluten-free pasta with a low glycemic index and high nutritional quality.
27603917	4	12	theme	Rheological	606:616	arg1	properties					618:627	Rheological properties	606:627	Rheological properties	606:627	The relationship between their structure, their cooking and Rheological properties and their in-vitro starch digestion was analyzed and compared to cereal gluten-free commercial pasta.
27603917	9	13	theme	high	1181:1184	arg1	0.85					1199:1202	0.85	1199:1202	0.85	1199:1202	Black-gram pasta, which is especially rich in soluble fibers, differed from faba and lentil pasta, with high springiness (0.85 vs. 0.75) and less loss during cooking.
27603917	9	13	theme	high	1181:1184	arg1	springiness					1186:1196	high springiness	1181:1196	high springiness (0.85 vs. 0.75)	1181:1212	Black-gram pasta, which is especially rich in soluble fibers, differed from faba and lentil pasta, with high springiness (0.85 vs. 0.75) and less loss during cooking.
27603917	9	13	theme	high	1181:1184	arg1	0.75					1208:1211	0.75	1208:1211	0.75	1208:1211	Black-gram pasta, which is especially rich in soluble fibers, differed from faba and lentil pasta, with high springiness (0.85 vs. 0.75) and less loss during cooking.
27603917	12	14	theme	trypsin	1634:1640	arg1	activity					1653:1660	trypsin inhibitory activity	1634:1660	trypsin inhibitory activity	1634:1660	Anti-nutritional factors in legumes, such as trypsin inhibitory activity and α-galactosides were reduced by up to 82% and 73%, respectively, by pasta processing and cooking.
27603917	4	15	theme	cereal	694:699	arg1	pasta					724:728	cereal gluten-free commercial pasta	694:728	cereal gluten-free commercial pasta	694:728	The relationship between their structure, their cooking and Rheological properties and their in-vitro starch digestion was analyzed and compared to cereal gluten-free commercial pasta.
27603917	14	16	theme	low	1933:1935	arg1	index					1946:1950	a low glycemic index	1931:1950	a low glycemic index	1931:1950	This study demonstrates the advantages of using legumes for the production of gluten-free pasta with a low glycemic index and high nutritional quality.
27603917	7	17	theme	cooking	981:987	arg1	step					989:992	the pasta cooking step	971:992	the pasta cooking step	971:992	They had a thick but weak protein network, which is built during the pasta cooking step.
27603917	13	18	theme	phytic	1817:1822	arg1	acid					1824:1827	phytic acid	1817:1827	phytic acid	1817:1827	However, these processing steps had a minor effect on phytic acid.
27603917	5	19	theme	inhibitory	739:748	arg1	activity					750:757	Trypsin inhibitory activity	731:757	Trypsin inhibitory activity	731:757	Trypsin inhibitory activity, phytic acid and α-galactosides were determined in flours and in cooked pasta.
27603917	7	20	theme	protein	932:938	arg1	network					940:946	a thick but weak protein network	915:946	a thick but weak protein network	915:946	They had a thick but weak protein network, which is built during the pasta cooking step.
27603917	0	21	dep	Pasta	108:112	arg1	Properties					55:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties	0:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free,	0:94	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	9	22	from	fibers	1131:1136	arg1	rich					1115:1118	rich	1115:1118	rich	1115:1118	Black-gram pasta, which is especially rich in soluble fibers, differed from faba and lentil pasta, with high springiness (0.85 vs. 0.75) and less loss during cooking.
27603917	0	23	theme	Protein	74:80	arg1	Properties					55:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties	0:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free,	0:94	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	11	24	theme	particular	1418:1427	arg1	composition					1429:1439	their particular composition	1412:1439	their particular composition	1412:1439	Interestingly, due to their particular composition and structure, lentil and faba pasta released their starch more slowly than the commercial gluten-free pasta during the in-vitro digestion process.
27603917	14	25	theme	gluten-free	1908:1918	arg1	pasta					1920:1924	gluten-free pasta	1908:1924	gluten-free pasta with a low glycemic index and high nutritional quality	1908:1979	This study demonstrates the advantages of using legumes for the production of gluten-free pasta with a low glycemic index and high nutritional quality.
27603917	13	26	contain	had	1795:1797	arg2	effect					1807:1812	a minor effect	1799:1812	a minor effect	1799:1812	However, these processing steps had a minor effect on phytic acid.
27603917	13	26	contain	had	1795:1797	arg1	steps					1789:1793	these processing steps	1772:1793	these processing steps	1772:1793	However, these processing steps had a minor effect on phytic acid.
27603917	2	27	theme	resistant	356:364	arg1	flours					332:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	27	theme	resistant	356:364	arg1	alternative					402:412	a good alternative	395:412	a good alternative for gluten-free pasta production	395:445	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	27	theme	resistant	356:364	arg1	minerals					377:384	minerals	377:384	minerals	377:384	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	27	theme	resistant	356:364	arg1	starch					366:371	resistant starch	356:371	resistant starch	356:371	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	0	28	theme	High	69:72	arg1	Protein					74:80	High Protein	69:80	High Protein	69:80	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	0	28	theme	High	69:72	arg1	Gluten					83:88	Gluten Free	83:93	Gluten Free	83:93	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	4	29	theme	commercial	713:722	arg1	pasta					724:728	cereal gluten-free commercial pasta	694:728	cereal gluten-free commercial pasta	694:728	The relationship between their structure, their cooking and Rheological properties and their in-vitro starch digestion was analyzed and compared to cereal gluten-free commercial pasta.
27603917	0	30	theme	Structural	0:9	arg1	Properties					55:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties	0:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free,	0:94	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	14	31	theme	nutritional	1961:1971	arg1	quality					1973:1979	high nutritional quality	1956:1979	high nutritional quality	1956:1979	This study demonstrates the advantages of using legumes for the production of gluten-free pasta with a low glycemic index and high nutritional quality.
27603917	0	32	theme	Culinary	12:19	arg1	Properties					55:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties	0:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free,	0:94	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	8	33	theme	pasta	1029:1033	arg1	springiness					1035:1045	pasta springiness	1029:1045	pasta springiness	1029:1045	This particular structure altered pasta springiness and increased cooking losses.
27603917	9	34	theme	Black-gram	1077:1086	arg1	pasta					1088:1092	Black-gram pasta	1077:1092	Black-gram pasta	1077:1092	Black-gram pasta, which is especially rich in soluble fibers, differed from faba and lentil pasta, with high springiness (0.85 vs. 0.75) and less loss during cooking.
27603917	0	35	theme	Nutritional	22:32	arg1	Properties					55:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties	0:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free,	0:94	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	2	36	from	fibers	348:353	arg1	rich					340:343	rich	340:343	rich	340:343	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	37	theme	gluten-free	418:428	arg1	production					436:445	gluten-free pasta production	418:445	gluten-free pasta production	418:445	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	14	38	theme	glycemic	1937:1944	arg1	index					1946:1950	a low glycemic index	1931:1950	a low glycemic index	1931:1950	This study demonstrates the advantages of using legumes for the production of gluten-free pasta with a low glycemic index and high nutritional quality.
27603917	2	39	theme	gluten-free	313:323	arg1	flours					332:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	39	theme	gluten-free	313:323	arg1	alternative					402:412	a good alternative	395:412	a good alternative for gluten-free pasta production	395:445	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	39	theme	gluten-free	313:323	arg1	minerals					377:384	minerals	377:384	minerals	377:384	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	39	theme	gluten-free	313:323	arg1	starch					366:371	resistant starch	356:371	resistant starch	356:371	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	0	40	theme	Anti-Nutritional	38:53	arg1	Properties					55:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties	0:64	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free,	0:94	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	1	41	theme	gluten-intolerant	274:290	arg1	people					292:297	gluten-intolerant people	274:297	gluten-intolerant people	274:297	Wheat pasta has a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index, but is nevertheless unsuitable for gluten-intolerant people.
27603917	8	42	theme	cooking	1061:1067	arg1	losses					1069:1074	cooking losses	1061:1074	cooking losses	1061:1074	This particular structure altered pasta springiness and increased cooking losses.
27603917	2	43	theme	High	300:303	arg1	flours					332:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	43	theme	High	300:303	arg1	alternative					402:412	a good alternative	395:412	a good alternative for gluten-free pasta production	395:445	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	43	theme	High	300:303	arg1	minerals					377:384	minerals	377:384	minerals	377:384	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	43	theme	High	300:303	arg1	starch					366:371	resistant starch	356:371	resistant starch	356:371	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	1	44	theme	starch	188:193	arg1	granules					195:202	starch granules	188:202	starch granules resulting in a low glycemic index	188:236	Wheat pasta has a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index, but is nevertheless unsuitable for gluten-intolerant people.
27603917	12	45	dep	82	1703:1704	arg1	to					1700:1701	to	1700:1701	to	1700:1701	Anti-nutritional factors in legumes, such as trypsin inhibitory activity and α-galactosides were reduced by up to 82% and 73%, respectively, by pasta processing and cooking.
27603917	2	46	theme	rich	340:343	arg1	flours					332:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	46	theme	rich	340:343	arg1	alternative					402:412	a good alternative	395:412	a good alternative for gluten-free pasta production	395:445	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	46	theme	rich	340:343	arg1	minerals					377:384	minerals	377:384	minerals	377:384	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	46	theme	rich	340:343	arg1	starch					366:371	resistant starch	356:371	resistant starch	356:371	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	11	47	theme	digestion	1570:1578	arg1	process					1580:1586	the in-vitro digestion process	1557:1586	the in-vitro digestion process	1557:1586	Interestingly, due to their particular composition and structure, lentil and faba pasta released their starch more slowly than the commercial gluten-free pasta during the in-vitro digestion process.
27603917	9	48	theme	soluble	1123:1129	arg1	fibers					1131:1136	soluble fibers	1123:1136	soluble fibers	1123:1136	Black-gram pasta, which is especially rich in soluble fibers, differed from faba and lentil pasta, with high springiness (0.85 vs. 0.75) and less loss during cooking.
27603917	6	49	theme	resistant	877:885	arg1	starch					887:892	resistant starch	877:892	resistant starch	877:892	All legume pasta were rich in protein, resistant starch and fibers.
27603917	6	50	from	rich	860:863	arg1	fibers					898:903	fibers	898:903	fibers	898:903	All legume pasta were rich in protein, resistant starch and fibers.
27603917	6	50	from	rich	860:863	arg1	protein					868:874	protein	868:874	protein	868:874	All legume pasta were rich in protein, resistant starch and fibers.
27603917	6	50	from	rich	860:863	arg1	starch					887:892	resistant starch	877:892	resistant starch	877:892	All legume pasta were rich in protein, resistant starch and fibers.
27603917	10	51	theme	legume	1308:1313	arg1	pasta					1315:1319	all the legume pasta	1300:1319	all the legume pasta	1300:1319	In comparison to a commercial cereal gluten-free pasta, all the legume pasta lost less material during cooking but was less cohesive and springy.
27603917	10	51	theme	legume	1308:1313	arg1	cohesive					1368:1375	cohesive	1368:1375	cohesive	1368:1375	In comparison to a commercial cereal gluten-free pasta, all the legume pasta lost less material during cooking but was less cohesive and springy.
27603917	1	52	theme	glycemic	223:230	arg1	index					232:236	a low glycemic index	217:236	a low glycemic index	217:236	Wheat pasta has a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index, but is nevertheless unsuitable for gluten-intolerant people.
27603917	11	53	theme	commercial	1521:1530	arg1	pasta					1544:1548	the commercial gluten-free pasta	1517:1548	the commercial gluten-free pasta	1517:1548	Interestingly, due to their particular composition and structure, lentil and faba pasta released their starch more slowly than the commercial gluten-free pasta during the in-vitro digestion process.
27603917	6	54	from	protein	868:874	arg1	rich					860:863	rich	860:863	rich	860:863	All legume pasta were rich in protein, resistant starch and fibers.
27603917	1	55	contain	has	127:129	arg1	pasta					121:125	Wheat pasta	115:125	Wheat pasta	115:125	Wheat pasta has a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index, but is nevertheless unsuitable for gluten-intolerant people.
27603917	1	55	contain	has	127:129	arg2	structure					141:149	a compact structure	131:149	a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index	131:236	Wheat pasta has a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index, but is nevertheless unsuitable for gluten-intolerant people.
27603917	1	56	theme	compact	133:139	arg1	structure					141:149	a compact structure	131:149	a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index	131:236	Wheat pasta has a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index, but is nevertheless unsuitable for gluten-intolerant people.
27603917	10	57	dep	pasta	1293:1297	arg1	comparison					1247:1256	comparison	1247:1256	comparison	1247:1256	In comparison to a commercial cereal gluten-free pasta, all the legume pasta lost less material during cooking but was less cohesive and springy.
27603917	2	58	from	rich	340:343	arg1	fibers					348:353	fibers	348:353	fibers	348:353	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	9	59	from	rich	1115:1118	arg1	fibers					1131:1136	soluble fibers	1123:1136	soluble fibers	1123:1136	Black-gram pasta, which is especially rich in soluble fibers, differed from faba and lentil pasta, with high springiness (0.85 vs. 0.75) and less loss during cooking.
27603917	7	60	contain	had	911:913	arg1	They					906:909	They	906:909	They	906:909	They had a thick but weak protein network, which is built during the pasta cooking step.
27603917	7	60	contain	had	911:913	arg2	network					940:946	a thick but weak protein network	915:946	a thick but weak protein network	915:946	They had a thick but weak protein network, which is built during the pasta cooking step.
27603917	11	61	theme	faba	1467:1470	arg1	pasta					1472:1476	faba pasta	1467:1476	faba pasta	1467:1476	Interestingly, due to their particular composition and structure, lentil and faba pasta released their starch more slowly than the commercial gluten-free pasta during the in-vitro digestion process.
27603917	0	62	theme	Legume	101:106	arg1	Pasta					108:112	100% Legume Pasta	96:112	100% Legume Pasta	96:112	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	5	63	theme	cooked	824:829	arg1	pasta					831:835	cooked pasta	824:835	cooked pasta	824:835	Trypsin inhibitory activity, phytic acid and α-galactosides were determined in flours and in cooked pasta.
27603917	13	64	theme	minor	1801:1805	arg1	effect					1807:1812	a minor effect	1799:1812	a minor effect	1799:1812	However, these processing steps had a minor effect on phytic acid.
27603917	7	65	theme	pasta	975:979	arg1	step					989:992	the pasta cooking step	971:992	the pasta cooking step	971:992	They had a thick but weak protein network, which is built during the pasta cooking step.
27603917	5	66	theme	Trypsin	731:737	arg1	activity					750:757	Trypsin inhibitory activity	731:757	Trypsin inhibitory activity	731:757	Trypsin inhibitory activity, phytic acid and α-galactosides were determined in flours and in cooked pasta.
27603917	1	67	theme	Wheat	115:119	arg1	pasta					121:125	Wheat pasta	115:125	Wheat pasta	115:125	Wheat pasta has a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index, but is nevertheless unsuitable for gluten-intolerant people.
27603917	12	68	theme	inhibitory	1642:1651	arg1	activity					1653:1660	trypsin inhibitory activity	1634:1660	trypsin inhibitory activity	1634:1660	Anti-nutritional factors in legumes, such as trypsin inhibitory activity and α-galactosides were reduced by up to 82% and 73%, respectively, by pasta processing and cooking.
27603917	4	69	theme	starch	648:653	arg1	digestion					655:663	their in-vitro starch digestion	633:663	their in-vitro starch digestion	633:663	The relationship between their structure, their cooking and Rheological properties and their in-vitro starch digestion was analyzed and compared to cereal gluten-free commercial pasta.
27603917	13	70	theme	processing	1778:1787	arg1	steps					1789:1793	these processing steps	1772:1793	these processing steps	1772:1793	However, these processing steps had a minor effect on phytic acid.
27603917	0	71	theme	Free	90:93	arg1	Protein					74:80	High Protein	69:80	High Protein	69:80	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	0	71	theme	Free	90:93	arg1	Gluten					83:88	Gluten Free	83:93	Gluten Free	83:93	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	7	72	theme	weak	927:930	arg1	network					940:946	a thick but weak protein network	915:946	a thick but weak protein network	915:946	They had a thick but weak protein network, which is built during the pasta cooking step.
27603917	14	73	theme	pasta	1920:1924	arg1	production					1894:1903	the production	1890:1903	the production of gluten-free pasta with a low glycemic index and high nutritional quality	1890:1979	This study demonstrates the advantages of using legumes for the production of gluten-free pasta with a low glycemic index and high nutritional quality.
27603917	0	74	theme	100	96:98	arg1	%					99:99	%	99:99	%	99:99	Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
27603917	8	75	theme	particular	1000:1009	arg1	structure					1011:1019	This particular structure	995:1019	This particular structure	995:1019	This particular structure altered pasta springiness and increased cooking losses.
27603917	7	76	theme	thick	917:921	arg1	network					940:946	a thick but weak protein network	915:946	a thick but weak protein network	915:946	They had a thick but weak protein network, which is built during the pasta cooking step.
27603917	12	77	theme	Anti-nutritional	1589:1604	arg1	factors					1606:1612	Anti-nutritional factors	1589:1612	Anti-nutritional factors in legumes, such as trypsin inhibitory activity and α-galactosides	1589:1679	Anti-nutritional factors in legumes, such as trypsin inhibitory activity and α-galactosides were reduced by up to 82% and 73%, respectively, by pasta processing and cooking.
27603917	2	78	theme	good	397:400	arg1	flours					332:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	78	theme	good	397:400	arg1	alternative					402:412	a good alternative	395:412	a good alternative for gluten-free pasta production	395:445	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	78	theme	good	397:400	arg1	minerals					377:384	minerals	377:384	minerals	377:384	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	78	theme	good	397:400	arg1	starch					366:371	resistant starch	356:371	resistant starch	356:371	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	4	79	theme	gluten-free	701:711	arg1	pasta					724:728	cereal gluten-free commercial pasta	694:728	cereal gluten-free commercial pasta	694:728	The relationship between their structure, their cooking and Rheological properties and their in-vitro starch digestion was analyzed and compared to cereal gluten-free commercial pasta.
27603917	14	80	theme	high	1956:1959	arg1	quality					1973:1979	high nutritional quality	1956:1979	high nutritional quality	1956:1979	This study demonstrates the advantages of using legumes for the production of gluten-free pasta with a low glycemic index and high nutritional quality.
27603917	5	81	theme	phytic	760:765	arg1	acid					767:770	phytic acid	760:770	phytic acid	760:770	Trypsin inhibitory activity, phytic acid and α-galactosides were determined in flours and in cooked pasta.
27603917	6	82	theme	legume	842:847	arg1	pasta					849:853	All legume pasta	838:853	All legume pasta	838:853	All legume pasta were rich in protein, resistant starch and fibers.
27603917	2	83	theme	pasta	430:434	arg1	production					436:445	gluten-free pasta production	418:445	gluten-free pasta production	418:445	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	10	84	theme	commercial	1263:1272	arg1	pasta					1293:1297	a commercial cereal gluten-free pasta	1261:1297	a commercial cereal gluten-free pasta	1261:1297	In comparison to a commercial cereal gluten-free pasta, all the legume pasta lost less material during cooking but was less cohesive and springy.
27603917	6	85	from	fibers	898:903	arg1	rich					860:863	rich	860:863	rich	860:863	All legume pasta were rich in protein, resistant starch and fibers.
27603917	2	86	theme	protein	305:311	arg1	flours					332:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours	300:337	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	86	theme	protein	305:311	arg1	alternative					402:412	a good alternative	395:412	a good alternative for gluten-free pasta production	395:445	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	86	theme	protein	305:311	arg1	minerals					377:384	minerals	377:384	minerals	377:384	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	2	86	theme	protein	305:311	arg1	starch					366:371	resistant starch	356:371	resistant starch	356:371	High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production.
27603917	11	87	theme	gluten-free	1532:1542	arg1	pasta					1544:1548	the commercial gluten-free pasta	1517:1548	the commercial gluten-free pasta	1517:1548	Interestingly, due to their particular composition and structure, lentil and faba pasta released their starch more slowly than the commercial gluten-free pasta during the in-vitro digestion process.
27603917	12	88	theme	pasta	1733:1737	arg1	processing					1739:1748	pasta processing	1733:1748	pasta processing	1733:1748	Anti-nutritional factors in legumes, such as trypsin inhibitory activity and α-galactosides were reduced by up to 82% and 73%, respectively, by pasta processing and cooking.
27603917	11	89	theme	in-vitro	1561:1568	arg1	process					1580:1586	the in-vitro digestion process	1557:1586	the in-vitro digestion process	1557:1586	Interestingly, due to their particular composition and structure, lentil and faba pasta released their starch more slowly than the commercial gluten-free pasta during the in-vitro digestion process.
27603917	10	90	theme	gluten-free	1281:1291	arg1	pasta					1293:1297	a commercial cereal gluten-free pasta	1261:1297	a commercial cereal gluten-free pasta	1261:1297	In comparison to a commercial cereal gluten-free pasta, all the legume pasta lost less material during cooking but was less cohesive and springy.
25375330	7	0	with	size	997:1000	arg1	excipients					1043:1052	these excipients	1037:1052	these excipients	1037:1052	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	1	1	theme	nanoparticle	157:168	arg1	preparation					170:180	nanoparticle preparation	157:180	nanoparticle preparation	157:180	One approach for the enhancement of oral drug bioavailability is the technique of nanoparticle preparation.
25375330	5	2	theme	mid-infrared	732:743	arg1	spectroscopy					745:756	mid-infrared spectroscopy	732:756	mid-infrared spectroscopy	732:756	Fourier transform mid-infrared spectroscopy was used for verification of the composition of the samples.
25375330	1	3	theme	preparation	170:180	arg1	approach					79:86	One approach	75:86	One approach for the enhancement of oral drug bioavailability	75:135	One approach for the enhancement of oral drug bioavailability is the technique of nanoparticle preparation.
25375330	1	3	theme	preparation	170:180	arg1	technique					144:152	the technique	140:152	the technique of nanoparticle preparation	140:180	One approach for the enhancement of oral drug bioavailability is the technique of nanoparticle preparation.
25375330	5	4	theme	samples	810:816	arg1	composition					791:801	the composition	787:801	the composition of the samples	787:816	Fourier transform mid-infrared spectroscopy was used for verification of the composition of the samples.
25375330	3	5	with	technique	425:433	arg1	polysorbate					464:474	polysorbate	464:474	polysorbate	464:474	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	3	5	with	technique	425:433	arg1	sulfate					455:461	sodium dodecyl sulfate	440:461	sodium dodecyl sulfate	440:461	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	3	5	with	technique	425:433	arg1	dextran					543:549	sodium carboxymethyl dextran	522:549	sodium carboxymethyl dextran	522:549	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	3	5	with	technique	425:433	arg1	cellulose					508:516	sodium carboxymethyl cellulose	487:516	sodium carboxymethyl cellulose	487:516	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	3	5	with	technique	425:433	arg1	macrogol					477:484	macrogol	477:484	macrogol	477:484	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	4	6	theme	dynamic	655:661	arg1	scattering					669:678	dynamic light scattering	655:678	dynamic light scattering	655:678	The prepared samples were characterized by dynamic light scattering and scanning electron microscopy.
25375330	7	7	theme	risedronate	1020:1030	arg1	samples					1009:1015	the samples	1005:1015	the samples of risedronate	1005:1030	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	2	8	theme	water	295:299	arg1	solubility					301:310	high water solubility	290:310	high water solubility	290:310	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	7	9	dep	10.5	1073:1076	arg1	to					1070:1071	to	1070:1071	to	1070:1071	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	3	10	theme	risedronate	365:375	arg1	sodium					377:382	risedronate sodium	365:382	risedronate sodium	365:382	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	3	11	theme	sodium	522:527	arg1	dextran					543:549	sodium carboxymethyl dextran	522:549	sodium carboxymethyl dextran	522:549	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	4	12	theme	electron	693:700	arg1	microscopy					702:711	scanning electron microscopy	684:711	scanning electron microscopy	684:711	The prepared samples were characterized by dynamic light scattering and scanning electron microscopy.
25375330	2	13	theme	high	290:293	arg1	solubility					301:310	high water solubility	290:310	high water solubility	290:310	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	3	14	theme	sodium	377:382	arg1	samples					354:360	Eighteen samples	345:360	Eighteen samples of risedronate sodium	345:382	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	3	15	theme	sodium	440:445	arg1	sulfate					455:461	sodium dodecyl sulfate	440:461	sodium dodecyl sulfate	440:461	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	7	16	theme	samples	1009:1015	arg1	size					997:1000	the particle size	984:1000	the particle size of the samples of risedronate with these excipients	984:1052	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	3	17	theme	solvent	405:411	arg1	technique					425:433	the solvent evaporation technique	401:433	the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations	401:609	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	4	18	theme	prepared	616:623	arg1	samples					625:631	The prepared samples	612:631	The prepared samples	612:631	The prepared samples were characterized by dynamic light scattering and scanning electron microscopy.
25375330	3	19	theme	evaporation	413:423	arg1	technique					425:433	the solvent evaporation technique	401:433	the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations	401:609	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	2	20	theme	intestinal	320:329	arg1	permeability					331:342	low intestinal permeability	316:342	low intestinal permeability	316:342	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	2	21	theme	low	316:318	arg1	permeability					331:342	low intestinal permeability	316:342	low intestinal permeability	316:342	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	3	22	theme	sodium	487:492	arg1	cellulose					508:516	sodium carboxymethyl cellulose	487:516	sodium carboxymethyl cellulose	487:516	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	0	23	theme	risedronate	15:25	arg1	Preparation					0:10	Preparation	0:10	Preparation of risedronate	0:25	Preparation of risedronate nanoparticles by solvent evaporation technique.
25375330	5	24	theme	composition	791:801	arg1	verification					771:782	verification	771:782	verification of the composition of the samples	771:816	Fourier transform mid-infrared spectroscopy was used for verification of the composition of the samples.
25375330	1	25	theme	oral	111:114	arg1	bioavailability					121:135	oral drug bioavailability	111:135	oral drug bioavailability	111:135	One approach for the enhancement of oral drug bioavailability is the technique of nanoparticle preparation.
25375330	7	26	theme	sodium	891:896	arg1	dextran					912:918	sodium carboxymethyl dextran	891:918	sodium carboxymethyl dextran	891:918	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	7	26	theme	sodium	891:896	arg1	excipients					972:981	the most favourable excipients	952:981	the most favourable excipients	952:981	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	7	26	theme	sodium	891:896	arg1	Polysorbate					878:888	Polysorbate	878:888	Polysorbate	878:888	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	7	26	theme	sodium	891:896	arg1	macrogol					924:931	macrogol	924:931	macrogol	924:931	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	3	27	theme	carboxymethyl	494:506	arg1	cellulose					508:516	sodium carboxymethyl cellulose	487:516	sodium carboxymethyl cellulose	487:516	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	0	28	theme	solvent	44:50	arg1	technique					64:72	solvent evaporation technique	44:72	solvent evaporation technique	44:72	Preparation of risedronate nanoparticles by solvent evaporation technique.
25375330	3	29	theme	carboxymethyl	529:541	arg1	dextran					543:549	sodium carboxymethyl dextran	522:549	sodium carboxymethyl dextran	522:549	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	7	30	theme	carboxymethyl	898:910	arg1	dextran					912:918	sodium carboxymethyl dextran	891:918	sodium carboxymethyl dextran	891:918	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	7	30	theme	carboxymethyl	898:910	arg1	excipients					972:981	the most favourable excipients	952:981	the most favourable excipients	952:981	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	7	30	theme	carboxymethyl	898:910	arg1	Polysorbate					878:888	Polysorbate	878:888	Polysorbate	878:888	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	7	30	theme	carboxymethyl	898:910	arg1	macrogol					924:931	macrogol	924:931	macrogol	924:931	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	3	31	theme	dodecyl	447:453	arg1	sulfate					455:461	sodium dodecyl sulfate	440:461	sodium dodecyl sulfate	440:461	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	5	32	dep	Fourier	714:720	arg1	transform					722:730	transform	722:730	transform mid-infrared spectroscopy	722:756	Fourier transform mid-infrared spectroscopy was used for verification of the composition of the samples.
25375330	2	33	theme	Risedronate	183:193	arg1	sodium					195:200	Risedronate sodium	183:200	Risedronate sodium (Biopharmaceutical Classification System Class III)	183:252	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	2	33	theme	Risedronate	183:193	arg1	compound					276:283	a model compound	268:283	a model compound with high water solubility and low intestinal permeability	268:342	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	2	33	theme	Risedronate	183:193	arg1	Class					243:247	Biopharmaceutical Classification System Class III	203:251	Biopharmaceutical Classification System Class III	203:251	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	6	34	theme	samples	848:854	arg1	nm					874:875	less than 200 nm	860:875	less than 200 nm	860:875	The particle size of sixteen samples was less than 200 nm.
25375330	6	34	theme	samples	848:854	arg1	size					832:835	The particle size	819:835	The particle size of sixteen samples	819:854	The particle size of sixteen samples was less than 200 nm.
25375330	1	35	theme	drug	116:119	arg1	bioavailability					121:135	oral drug bioavailability	111:135	oral drug bioavailability	111:135	One approach for the enhancement of oral drug bioavailability is the technique of nanoparticle preparation.
25375330	0	36	theme	evaporation	52:62	arg1	technique					64:72	solvent evaporation technique	44:72	solvent evaporation technique	44:72	Preparation of risedronate nanoparticles by solvent evaporation technique.
25375330	2	37	with	compound	276:283	arg1	solubility					301:310	high water solubility	290:310	high water solubility	290:310	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	2	37	with	compound	276:283	arg1	permeability					331:342	low intestinal permeability	316:342	low intestinal permeability	316:342	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	1	38	theme	bioavailability	121:135	arg1	enhancement					96:106	the enhancement	92:106	the enhancement of oral drug bioavailability	92:135	One approach for the enhancement of oral drug bioavailability is the technique of nanoparticle preparation.
25375330	7	39	theme	particle	988:995	arg1	size					997:1000	the particle size	984:1000	the particle size of the samples of risedronate with these excipients	984:1052	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	2	40	theme	System	236:241	arg1	sodium					195:200	Risedronate sodium	183:200	Risedronate sodium (Biopharmaceutical Classification System Class III)	183:252	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	2	40	theme	System	236:241	arg1	Class					243:247	Biopharmaceutical Classification System Class III	203:251	Biopharmaceutical Classification System Class III	203:251	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	2	41	theme	Classification	221:234	arg1	sodium					195:200	Risedronate sodium	183:200	Risedronate sodium (Biopharmaceutical Classification System Class III)	183:252	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	2	41	theme	Classification	221:234	arg1	Class					243:247	Biopharmaceutical Classification System Class III	203:251	Biopharmaceutical Classification System Class III	203:251	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	2	42	theme	model	270:274	arg1	sodium					195:200	Risedronate sodium	183:200	Risedronate sodium (Biopharmaceutical Classification System Class III)	183:252	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	2	42	theme	model	270:274	arg1	compound					276:283	a model compound	268:283	a model compound with high water solubility and low intestinal permeability	268:342	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	6	43	theme	particle	823:830	arg1	nm					874:875	less than 200 nm	860:875	less than 200 nm	860:875	The particle size of sixteen samples was less than 200 nm.
25375330	6	43	theme	particle	823:830	arg1	size					832:835	The particle size	819:835	The particle size of sixteen samples	819:854	The particle size of sixteen samples was less than 200 nm.
25375330	4	44	theme	light	663:667	arg1	scattering					669:678	dynamic light scattering	655:678	dynamic light scattering	655:678	The prepared samples were characterized by dynamic light scattering and scanning electron microscopy.
25375330	2	45	theme	Biopharmaceutical	203:219	arg1	sodium					195:200	Risedronate sodium	183:200	Risedronate sodium (Biopharmaceutical Classification System Class III)	183:252	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	2	45	theme	Biopharmaceutical	203:219	arg1	Class					243:247	Biopharmaceutical Classification System Class III	203:251	Biopharmaceutical Classification System Class III	203:251	Risedronate sodium (Biopharmaceutical Classification System Class III) was chosen as a model compound with high water solubility and low intestinal permeability.
25375330	3	46	theme	nanoparticle	554:565	arg1	stabilizers					567:577	nanoparticle stabilizers	554:577	nanoparticle stabilizers applied in three concentrations	554:609	Eighteen samples of risedronate sodium were prepared by the solvent evaporation technique with sodium dodecyl sulfate, polysorbate, macrogol, sodium carboxymethyl cellulose and sodium carboxymethyl dextran as nanoparticle stabilizers applied in three concentrations.
25375330	4	47	theme	scanning	684:691	arg1	microscopy					702:711	scanning electron microscopy	684:711	scanning electron microscopy	684:711	The prepared samples were characterized by dynamic light scattering and scanning electron microscopy.
25375330	5	48	used	used	762:765	arg2	Fourier					714:720	Fourier	714:720	Fourier transform mid-infrared spectroscopy	714:756	Fourier transform mid-infrared spectroscopy was used for verification of the composition of the samples.
25375330	7	49	theme	favourable	961:970	arg1	dextran					912:918	sodium carboxymethyl dextran	891:918	sodium carboxymethyl dextran	891:918	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	7	49	theme	favourable	961:970	arg1	excipients					972:981	the most favourable excipients	952:981	the most favourable excipients	952:981	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	7	49	theme	favourable	961:970	arg1	Polysorbate					878:888	Polysorbate	878:888	Polysorbate	878:888	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
25375330	7	49	theme	favourable	961:970	arg1	macrogol					924:931	macrogol	924:931	macrogol	924:931	Polysorbate, sodium carboxymethyl dextran and macrogol were determined as the most favourable excipients; the particle size of the samples of risedronate with these excipients ranged from 2.8 to 10.5 nm.
29191420	0	0	theme	starch	80:85	arg1	biocomposite					108:119	tapioca starch water hyacinth fiber biocomposite	72:119	tapioca starch water hyacinth fiber biocomposite	72:119	Effect of duration of sonication during gelatinization on properties of tapioca starch water hyacinth fiber biocomposite.
29191420	1	1	theme	biocomposite	161:172	arg1	properties					147:156	properties	147:156	properties of biocomposite sonicated during gelatinization	147:204	This paper characterizes properties of biocomposite sonicated during gelatinization.
29191420	0	2	theme	tapioca	72:78	arg1	biocomposite					108:119	tapioca starch water hyacinth fiber biocomposite	72:119	tapioca starch water hyacinth fiber biocomposite	72:119	Effect of duration of sonication during gelatinization on properties of tapioca starch water hyacinth fiber biocomposite.
29191420	3	3	from	vibrated	417:424	arg1	bath					443:446	an ultrasonic bath	429:446	an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min)	429:509	During gelatinization, the biocomposite was poured into a rectangular glass mold then vibrated in an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min).
29191420	0	4	from	Effect	0:5	arg1	properties					58:67	properties	58:67	properties of tapioca starch water hyacinth fiber biocomposite	58:119	Effect of duration of sonication during gelatinization on properties of tapioca starch water hyacinth fiber biocomposite.
29191420	0	5	theme	water	87:91	arg1	biocomposite					108:119	tapioca starch water hyacinth fiber biocomposite	72:119	tapioca starch water hyacinth fiber biocomposite	72:119	Effect of duration of sonication during gelatinization on properties of tapioca starch water hyacinth fiber biocomposite.
29191420	2	6	theme	water	303:307	arg1	WHF					325:327	water hyacinth fiber (WHF)	303:328	water hyacinth fiber (WHF)	303:328	The biocomposite consisted of tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF).
29191420	0	7	theme	biocomposite	108:119	arg1	properties					58:67	properties	58:67	properties of tapioca starch water hyacinth fiber biocomposite	58:119	Effect of duration of sonication during gelatinization on properties of tapioca starch water hyacinth fiber biocomposite.
29191420	8	8	theme	maximal	1049:1055	arg1	level					1057:1061	a maximal level	1047:1061	a maximal level	1047:1061	Moisture resistance of the biocomposite after vibration increased by around 25% reaching a maximal level after 30min.
29191420	0	9	theme	fiber	102:106	arg1	biocomposite					108:119	tapioca starch water hyacinth fiber biocomposite	72:119	tapioca starch water hyacinth fiber biocomposite	72:119	Effect of duration of sonication during gelatinization on properties of tapioca starch water hyacinth fiber biocomposite.
29191420	6	10	theme	tensile	817:823	arg1	strength					825:832	tensile strength	817:832	tensile strength (TS)	817:837	After this vibration duration, tensile strength (TS) and tensile modulus (TM) increased 83% and 108%.
29191420	6	10	theme	tensile	817:823	arg1	TS					835:836	TS	835:836	TS	835:836	After this vibration duration, tensile strength (TS) and tensile modulus (TM) increased 83% and 108%.
29191420	3	11	theme	rectangular	389:399	arg1	mold					407:410	a rectangular glass mold	387:410	a rectangular glass mold then vibrated in an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min)	387:509	During gelatinization, the biocomposite was poured into a rectangular glass mold then vibrated in an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min).
29191420	3	12	theme	glass	401:405	arg1	mold					407:410	a rectangular glass mold	387:410	a rectangular glass mold then vibrated in an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min)	387:509	During gelatinization, the biocomposite was poured into a rectangular glass mold then vibrated in an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min).
29191420	2	13	theme	WHF	325:327	arg1	fraction					291:298	10% volume fraction	280:298	10% volume fraction of water hyacinth fiber (WHF)	280:328	The biocomposite consisted of tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF).
29191420	3	14	theme	ultrasonic	432:441	arg1	bath					443:446	an ultrasonic bath	429:446	an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min)	429:509	During gelatinization, the biocomposite was poured into a rectangular glass mold then vibrated in an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min).
29191420	9	15	theme	Thermal	1076:1082	arg1	resistance					1084:1093	Thermal resistance	1076:1093	Thermal resistance of the vibrated biocomposites	1076:1123	Thermal resistance of the vibrated biocomposites was also increased.
29191420	0	16	theme	duration	10:17	arg1	Effect					0:5	Effect	0:5	Effect of duration of sonication during gelatinization on properties of tapioca starch water hyacinth fiber biocomposite.	0:120	Effect of duration of sonication during gelatinization on properties of tapioca starch water hyacinth fiber biocomposite.
29191420	2	17	theme	fiber	318:322	arg1	WHF					325:327	water hyacinth fiber (WHF)	303:328	water hyacinth fiber (WHF)	303:328	The biocomposite consisted of tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF).
29191420	3	18	theme	vibrated	417:424	arg1	mold					407:410	a rectangular glass mold	387:410	a rectangular glass mold then vibrated in an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min)	387:509	During gelatinization, the biocomposite was poured into a rectangular glass mold then vibrated in an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min).
29191420	4	19	theme	resulting	516:524	arg1	biocomposite					526:537	The resulting biocomposite	512:537	The resulting biocomposite	512:537	The resulting biocomposite was then dried in a drying oven at 50°C for 20h.
29191420	5	20	theme	optimal	648:654	arg1	properties					656:665	optimal properties	648:665	optimal properties	648:665	The results of this study indicate that a biocomposite with optimal properties can be produced using tapioca starch and WHF if the gelatinizing mixture is exposed to ultrasound vibration for 30min.
29191420	6	21	theme	vibration	797:805	arg1	duration					807:814	this vibration duration	792:814	this vibration duration	792:814	After this vibration duration, tensile strength (TS) and tensile modulus (TM) increased 83% and 108%.
29191420	5	22	with	biocomposite	630:641	arg1	properties					656:665	optimal properties	648:665	optimal properties	648:665	The results of this study indicate that a biocomposite with optimal properties can be produced using tapioca starch and WHF if the gelatinizing mixture is exposed to ultrasound vibration for 30min.
29191420	0	23	theme	sonication	22:31	arg1	duration					10:17	duration	10:17	duration of sonication during gelatinization	10:53	Effect of duration of sonication during gelatinization on properties of tapioca starch water hyacinth fiber biocomposite.
29191420	5	24	dep	starch	697:702	arg1	exposed					743:749	exposed	743:749	is exposed to ultrasound vibration for 30min	740:783	The results of this study indicate that a biocomposite with optimal properties can be produced using tapioca starch and WHF if the gelatinizing mixture is exposed to ultrasound vibration for 30min.
29191420	5	25	theme	gelatinizing	719:730	arg1	mixture					732:738	the gelatinizing mixture	715:738	the gelatinizing mixture	715:738	The results of this study indicate that a biocomposite with optimal properties can be produced using tapioca starch and WHF if the gelatinizing mixture is exposed to ultrasound vibration for 30min.
29191420	5	26	theme	study	608:612	arg1	results					592:598	The results	588:598	The results of this study	588:612	The results of this study indicate that a biocomposite with optimal properties can be produced using tapioca starch and WHF if the gelatinizing mixture is exposed to ultrasound vibration for 30min.
29191420	9	27	theme	vibrated	1102:1109	arg1	biocomposites					1111:1123	the vibrated biocomposites	1098:1123	the vibrated biocomposites	1098:1123	Thermal resistance of the vibrated biocomposites was also increased.
29191420	2	28	theme	based	252:256	arg1	plastic					258:264	tapioca starch based plastic	237:264	tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF)	237:328	The biocomposite consisted of tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF).
29191420	9	29	theme	biocomposites	1111:1123	arg1	resistance					1084:1093	Thermal resistance	1076:1093	Thermal resistance of the vibrated biocomposites	1076:1123	Thermal resistance of the vibrated biocomposites was also increased.
29191420	8	30	theme	Moisture	958:965	arg1	resistance					967:976	Moisture resistance	958:976	Moisture resistance of the biocomposite after vibration	958:1012	Moisture resistance of the biocomposite after vibration increased by around 25% reaching a maximal level after 30min.
29191420	2	31	theme	tapioca	237:243	arg1	plastic					258:264	tapioca starch based plastic	237:264	tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF)	237:328	The biocomposite consisted of tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF).
29191420	2	32	theme	starch	245:250	arg1	plastic					258:264	tapioca starch based plastic	237:264	tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF)	237:328	The biocomposite consisted of tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF).
29191420	7	33	theme	further	890:896	arg1	vibration					904:912	A further 60min vibration	888:912	A further 60min vibration	888:912	A further 60min vibration only increased the TS at 13% and TM at 23%.
29191420	2	34	theme	volume	284:289	arg1	fraction					291:298	10% volume fraction	280:298	10% volume fraction of water hyacinth fiber (WHF)	280:328	The biocomposite consisted of tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF).
29191420	7	35	theme	60min	898:902	arg1	vibration					904:912	A further 60min vibration	888:912	A further 60min vibration	888:912	A further 60min vibration only increased the TS at 13% and TM at 23%.
29191420	3	36	theme	varying	470:476	arg1	60min					504:508	0, 15, 30, and 60min	489:508	0, 15, 30, and 60min	489:508	During gelatinization, the biocomposite was poured into a rectangular glass mold then vibrated in an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min).
29191420	3	36	theme	varying	470:476	arg1	durations					478:486	varying durations	470:486	varying durations (0, 15, 30, and 60min)	470:509	During gelatinization, the biocomposite was poured into a rectangular glass mold then vibrated in an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min).
29191420	2	37	theme	%	282:282	arg1	fraction					291:298	10% volume fraction	280:298	10% volume fraction of water hyacinth fiber (WHF)	280:328	The biocomposite consisted of tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF).
29191420	5	38	theme	tapioca	689:695	arg1	starch					697:702	tapioca starch	689:702	tapioca starch	689:702	The results of this study indicate that a biocomposite with optimal properties can be produced using tapioca starch and WHF if the gelatinizing mixture is exposed to ultrasound vibration for 30min.
29191420	2	39	theme	10	280:281	arg1	%					282:282	%	282:282	%	282:282	The biocomposite consisted of tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF).
29191420	8	40	theme	biocomposite	985:996	arg1	resistance					967:976	Moisture resistance	958:976	Moisture resistance of the biocomposite after vibration	958:1012	Moisture resistance of the biocomposite after vibration increased by around 25% reaching a maximal level after 30min.
29191420	6	41	theme	tensile	843:849	arg1	TM					860:861	TM	860:861	TM	860:861	After this vibration duration, tensile strength (TS) and tensile modulus (TM) increased 83% and 108%.
29191420	6	41	theme	tensile	843:849	arg1	modulus					851:857	tensile modulus	843:857	tensile modulus (TM)	843:862	After this vibration duration, tensile strength (TS) and tensile modulus (TM) increased 83% and 108%.
29191420	5	42	theme	ultrasound	754:763	arg1	vibration					765:773	ultrasound vibration	754:773	ultrasound vibration	754:773	The results of this study indicate that a biocomposite with optimal properties can be produced using tapioca starch and WHF if the gelatinizing mixture is exposed to ultrasound vibration for 30min.
29191420	3	43	from	bath	443:446	arg1	vibrated					417:424	vibrated	417:424	vibrated	417:424	During gelatinization, the biocomposite was poured into a rectangular glass mold then vibrated in an ultrasonic bath using 40kHz, 250W for varying durations (0, 15, 30, and 60min).
27308202	8	0	theme	glycosylation	1249:1261	arg1	reactions					1263:1271	all glycosylation reactions	1245:1271	all glycosylation reactions	1245:1271	The stereoselective outcomes of all glycosylation reactions were very good.
27308202	1	1	theme	inter-batch	366:376	arg1	variations					378:387	inter-batch variations	366:387	inter-batch variations in oligosaccharide epitope structure	366:424	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	6	2	theme	reaction	1073:1080	arg1	conditions					1082:1091	recently developed reaction conditions	1054:1091	recently developed reaction conditions	1054:1091	A number of recently developed reaction conditions were used in the synthetic strategy.
27308202	2	3	theme	full-length	614:624	arg1	oligosaccharides					626:641	full-length oligosaccharides	614:641	full-length oligosaccharides	614:641	However, it has been shown in many cases that a synthetic fragment of appropriate structure conjugated to a protein can be an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides.
27308202	6	4	theme	conditions	1082:1091	arg1	number					1044:1049	A number	1042:1049	A number of recently developed reaction conditions	1042:1091	A number of recently developed reaction conditions were used in the synthetic strategy.
27308202	2	5	theme	effective	553:561	arg1	vaccine					563:569	an effective vaccine	550:569	an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides	550:641	However, it has been shown in many cases that a synthetic fragment of appropriate structure conjugated to a protein can be an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides.
27308202	2	5	theme	effective	553:561	arg1	fragment					485:492	a synthetic fragment	473:492	a synthetic fragment of appropriate structure conjugated to a protein	473:541	However, it has been shown in many cases that a synthetic fragment of appropriate structure conjugated to a protein can be an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides.
27308202	2	6	theme	structure	509:517	arg1	vaccine					563:569	an effective vaccine	550:569	an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides	550:641	However, it has been shown in many cases that a synthetic fragment of appropriate structure conjugated to a protein can be an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides.
27308202	2	6	theme	structure	509:517	arg1	fragment					485:492	a synthetic fragment	473:492	a synthetic fragment of appropriate structure conjugated to a protein	473:541	However, it has been shown in many cases that a synthetic fragment of appropriate structure conjugated to a protein can be an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides.
27308202	6	7	theme	developed	1063:1071	arg1	conditions					1082:1091	recently developed reaction conditions	1054:1091	recently developed reaction conditions	1054:1091	A number of recently developed reaction conditions were used in the synthetic strategy.
27308202	4	8	theme	repeat	838:843	arg1	unit					845:848	the repeat unit	834:848	the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53	834:909	Oligosaccharide fragments corresponding to the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53 were synthesized in good yield.
27308202	1	9	theme	live	299:302	arg1	strains					314:320	live bacterial strains	299:320	live bacterial strains	299:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	0	10	theme	Salmonella	101:110	arg1	O53					121:123	Salmonella enterica O53	101:123	Salmonella enterica O53	101:123	Convergent Synthesis of Oligosaccharide Fragments Corresponding to the Cell Wall O-Polysaccharide of Salmonella enterica O53.
27308202	1	11	theme	oligosaccharide	392:406	arg1	structure					416:424	oligosaccharide epitope structure	392:424	oligosaccharide epitope structure	392:424	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	6	12	used	used	1098:1101	arg2	number					1044:1049	A number	1042:1049	A number of recently developed reaction conditions	1042:1091	A number of recently developed reaction conditions were used in the synthetic strategy.
27308202	1	13	theme	bacterial	304:312	arg1	strains					314:320	live bacterial strains	299:320	live bacterial strains	299:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	14	theme	epitope	408:414	arg1	structure					416:424	oligosaccharide epitope structure	392:424	oligosaccharide epitope structure	392:424	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	4	15	theme	good	931:934	arg1	yield					936:940	good yield	931:940	good yield	931:940	Oligosaccharide fragments corresponding to the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53 were synthesized in good yield.
27308202	1	16	attach	presence	327:334	arg2	impurities					350:359	biological impurities	339:359	biological impurities	339:359	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	16	attach	presence	327:334	arg1	structure					416:424	oligosaccharide epitope structure	392:424	oligosaccharide epitope structure	392:424	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	7	17	theme	multiple	1183:1190	arg1	steps					1206:1210	the multiple glycosylation steps	1179:1210	the multiple glycosylation steps	1179:1210	A one-pot reaction scheme was also developed for the multiple glycosylation steps.
27308202	0	18	theme	O53	121:123	arg1	O-Polysaccharide					81:96	the Cell Wall O-Polysaccharide	67:96	the Cell Wall O-Polysaccharide of Salmonella enterica O53	67:123	Convergent Synthesis of Oligosaccharide Fragments Corresponding to the Cell Wall O-Polysaccharide of Salmonella enterica O53.
27308202	1	19	attach	isolated	197:204	arg1	variations					378:387	inter-batch variations	366:387	inter-batch variations in oligosaccharide epitope structure	366:424	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	19	attach	isolated	197:204	arg1	fermentation					221:232	bacterial fermentation	211:232	bacterial fermentation	211:232	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	19	attach	isolated	197:204	arg1	presence					327:334	the presence	323:334	the presence of biological impurities	323:359	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	19	attach	isolated	197:204	arg2	polysaccharides					181:195	polysaccharides	181:195	polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure	181:424	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	19	attach	isolated	197:204	arg1	approach					238:245	an approach	235:245	an approach with some significant drawbacks such as handling of live bacterial strains	235:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	20	from	presence	327:334	arg1	structure					416:424	oligosaccharide epitope structure	392:424	oligosaccharide epitope structure	392:424	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	5	21	used	used	994:997	arg2	strategies					978:987	block glycosylation strategies	958:987	block glycosylation strategies	958:987	Sequential and block glycosylation strategies were used for the synthesis of the target compounds.
27308202	5	21	used	used	994:997	arg2	Sequential					943:952	Sequential	943:952	Sequential	943:952	Sequential and block glycosylation strategies were used for the synthesis of the target compounds.
27308202	0	22	dep	Salmonella	101:110	arg1	enterica					112:119	enterica	112:119	enterica	112:119	Convergent Synthesis of Oligosaccharide Fragments Corresponding to the Cell Wall O-Polysaccharide of Salmonella enterica O53.
27308202	1	23	theme	bacterial	211:219	arg1	fermentation					221:232	bacterial fermentation	211:232	bacterial fermentation	211:232	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	2	24	theme	synthetic	475:483	arg1	vaccine					563:569	an effective vaccine	550:569	an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides	550:641	However, it has been shown in many cases that a synthetic fragment of appropriate structure conjugated to a protein can be an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides.
27308202	2	24	theme	synthetic	475:483	arg1	fragment					485:492	a synthetic fragment	473:492	a synthetic fragment of appropriate structure conjugated to a protein	473:541	However, it has been shown in many cases that a synthetic fragment of appropriate structure conjugated to a protein can be an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides.
27308202	1	25	with	presence	327:334	arg1	drawbacks					269:277	some significant drawbacks	252:277	some significant drawbacks such as handling of live bacterial strains	252:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	25	with	presence	327:334	arg1	handling					287:294	handling	287:294	handling of live bacterial strains	287:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	5	26	theme	target	1024:1029	arg1	compounds					1031:1039	the target compounds	1020:1039	the target compounds	1020:1039	Sequential and block glycosylation strategies were used for the synthesis of the target compounds.
27308202	5	27	theme	block	958:962	arg1	strategies					978:987	block glycosylation strategies	958:987	block glycosylation strategies	958:987	Sequential and block glycosylation strategies were used for the synthesis of the target compounds.
27308202	0	28	theme	Convergent	0:9	arg1	Synthesis					11:19	Convergent Synthesis	0:19	Convergent Synthesis of Oligosaccharide Fragments	0:48	Convergent Synthesis of Oligosaccharide Fragments Corresponding to the Cell Wall O-Polysaccharide of Salmonella enterica O53.
27308202	1	29	theme	strains	314:320	arg1	handling					287:294	handling	287:294	handling of live bacterial strains	287:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	5	30	theme	compounds	1031:1039	arg1	synthesis					1007:1015	the synthesis	1003:1015	the synthesis of the target compounds	1003:1039	Sequential and block glycosylation strategies were used for the synthesis of the target compounds.
27308202	5	31	theme	glycosylation	964:976	arg1	strategies					978:987	block glycosylation strategies	958:987	block glycosylation strategies	958:987	Sequential and block glycosylation strategies were used for the synthesis of the target compounds.
27308202	0	32	theme	Oligosaccharide	24:38	arg1	Fragments					40:48	Oligosaccharide Fragments	24:48	Oligosaccharide Fragments	24:48	Convergent Synthesis of Oligosaccharide Fragments Corresponding to the Cell Wall O-Polysaccharide of Salmonella enterica O53.
27308202	2	33	theme	many	457:460	arg1	cases					462:466	many cases	457:466	many cases	457:466	However, it has been shown in many cases that a synthetic fragment of appropriate structure conjugated to a protein can be an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides.
27308202	4	34	theme	wall	862:865	arg1	O-antigen					867:875	the cell wall O-antigen	853:875	the cell wall O-antigen of Salmonella enterica strain O53	853:909	Oligosaccharide fragments corresponding to the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53 were synthesized in good yield.
27308202	2	35	theme	appropriate	497:507	arg1	structure					509:517	appropriate structure	497:517	appropriate structure conjugated to a protein	497:541	However, it has been shown in many cases that a synthetic fragment of appropriate structure conjugated to a protein can be an effective vaccine that circumvents the shortcomings of using full-length oligosaccharides.
27308202	1	36	from	variations	378:387	arg1	structure					416:424	oligosaccharide epitope structure	392:424	oligosaccharide epitope structure	392:424	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	4	37	theme	O53	907:909	arg1	O-antigen					867:875	the cell wall O-antigen	853:875	the cell wall O-antigen of Salmonella enterica strain O53	853:909	Oligosaccharide fragments corresponding to the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53 were synthesized in good yield.
27308202	0	38	theme	Fragments	40:48	arg1	Synthesis					11:19	Convergent Synthesis	0:19	Convergent Synthesis of Oligosaccharide Fragments	0:48	Convergent Synthesis of Oligosaccharide Fragments Corresponding to the Cell Wall O-Polysaccharide of Salmonella enterica O53.
27308202	1	39	with	approach	238:245	arg1	drawbacks					269:277	some significant drawbacks	252:277	some significant drawbacks such as handling of live bacterial strains	252:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	39	with	approach	238:245	arg1	handling					287:294	handling	287:294	handling of live bacterial strains	287:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	4	40	theme	strain	900:905	arg1	O53					907:909	Salmonella enterica strain O53	880:909	Salmonella enterica strain O53	880:909	Oligosaccharide fragments corresponding to the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53 were synthesized in good yield.
27308202	1	41	theme	biological	339:348	arg1	impurities					350:359	biological impurities	339:359	biological impurities	339:359	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	42	with	fermentation	221:232	arg1	drawbacks					269:277	some significant drawbacks	252:277	some significant drawbacks such as handling of live bacterial strains	252:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	42	with	fermentation	221:232	arg1	handling					287:294	handling	287:294	handling of live bacterial strains	287:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	3	43	theme	glycoconjugate	695:708	arg1	derivatives					710:720	glycoconjugate derivatives	695:720	glycoconjugate derivatives	695:720	The development of synthetic strategies to prepare glycoconjugate derivatives against pathogenic bacterial strains is therefore of great interest.
27308202	8	44	theme	stereoselective	1217:1231	arg1	outcomes					1233:1240	The stereoselective outcomes	1213:1240	The stereoselective outcomes of all glycosylation reactions	1213:1271	The stereoselective outcomes of all glycosylation reactions were very good.
27308202	8	44	theme	stereoselective	1217:1231	arg1	good					1283:1286	good	1283:1286	good	1283:1286	The stereoselective outcomes of all glycosylation reactions were very good.
27308202	1	45	theme	Conventional	126:137	arg1	vaccines					154:161	Conventional glycoconjugate vaccines	126:161	Conventional glycoconjugate vaccines	126:161	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	46	theme	impurities	350:359	arg1	fermentation					221:232	bacterial fermentation	211:232	bacterial fermentation	211:232	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	46	theme	impurities	350:359	arg1	approach					238:245	an approach	235:245	an approach with some significant drawbacks such as handling of live bacterial strains	235:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	46	theme	impurities	350:359	arg1	presence					327:334	the presence	323:334	the presence of biological impurities	323:359	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	46	theme	impurities	350:359	arg1	variations					378:387	inter-batch variations	366:387	inter-batch variations in oligosaccharide epitope structure	366:424	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	7	47	theme	reaction	1140:1147	arg1	scheme					1149:1154	A one-pot reaction scheme	1130:1154	A one-pot reaction scheme	1130:1154	A one-pot reaction scheme was also developed for the multiple glycosylation steps.
27308202	7	48	theme	glycosylation	1192:1204	arg1	steps					1206:1210	the multiple glycosylation steps	1179:1210	the multiple glycosylation steps	1179:1210	A one-pot reaction scheme was also developed for the multiple glycosylation steps.
27308202	1	49	theme	glycoconjugate	139:152	arg1	vaccines					154:161	Conventional glycoconjugate vaccines	126:161	Conventional glycoconjugate vaccines	126:161	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	4	50	theme	Salmonella	880:889	arg1	O53					907:909	Salmonella enterica strain O53	880:909	Salmonella enterica strain O53	880:909	Oligosaccharide fragments corresponding to the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53 were synthesized in good yield.
27308202	4	51	theme	Oligosaccharide	791:805	arg1	fragments					807:815	Oligosaccharide fragments	791:815	Oligosaccharide fragments corresponding to the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53	791:909	Oligosaccharide fragments corresponding to the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53 were synthesized in good yield.
27308202	3	52	theme	strategies	673:682	arg1	development					648:658	The development	644:658	The development of synthetic strategies to prepare glycoconjugate derivatives against pathogenic bacterial strains	644:757	The development of synthetic strategies to prepare glycoconjugate derivatives against pathogenic bacterial strains is therefore of great interest.
27308202	1	53	from	approach	238:245	arg1	structure					416:424	oligosaccharide epitope structure	392:424	oligosaccharide epitope structure	392:424	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	54	theme	significant	257:267	arg1	drawbacks					269:277	some significant drawbacks	252:277	some significant drawbacks such as handling of live bacterial strains	252:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	54	theme	significant	257:267	arg1	handling					287:294	handling	287:294	handling of live bacterial strains	287:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	4	55	theme	cell	857:860	arg1	O-antigen					867:875	the cell wall O-antigen	853:875	the cell wall O-antigen of Salmonella enterica strain O53	853:909	Oligosaccharide fragments corresponding to the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53 were synthesized in good yield.
27308202	3	56	theme	pathogenic	730:739	arg1	strains					751:757	pathogenic bacterial strains	730:757	pathogenic bacterial strains	730:757	The development of synthetic strategies to prepare glycoconjugate derivatives against pathogenic bacterial strains is therefore of great interest.
27308202	4	57	theme	O-antigen	867:875	arg1	unit					845:848	the repeat unit	834:848	the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53	834:909	Oligosaccharide fragments corresponding to the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53 were synthesized in good yield.
27308202	1	58	from	fermentation	221:232	arg1	structure					416:424	oligosaccharide epitope structure	392:424	oligosaccharide epitope structure	392:424	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	6	59	theme	synthetic	1110:1118	arg1	strategy					1120:1127	the synthetic strategy	1106:1127	the synthetic strategy	1106:1127	A number of recently developed reaction conditions were used in the synthetic strategy.
27308202	0	60	theme	Wall	76:79	arg1	O-Polysaccharide					81:96	the Cell Wall O-Polysaccharide	67:96	the Cell Wall O-Polysaccharide of Salmonella enterica O53	67:123	Convergent Synthesis of Oligosaccharide Fragments Corresponding to the Cell Wall O-Polysaccharide of Salmonella enterica O53.
27308202	3	61	theme	bacterial	741:749	arg1	strains					751:757	pathogenic bacterial strains	730:757	pathogenic bacterial strains	730:757	The development of synthetic strategies to prepare glycoconjugate derivatives against pathogenic bacterial strains is therefore of great interest.
27308202	7	62	theme	one-pot	1132:1138	arg1	scheme					1149:1154	A one-pot reaction scheme	1130:1154	A one-pot reaction scheme	1130:1154	A one-pot reaction scheme was also developed for the multiple glycosylation steps.
27308202	4	63	dep	Salmonella	880:889	arg1	enterica					891:898	enterica	891:898	enterica	891:898	Oligosaccharide fragments corresponding to the repeat unit of the cell wall O-antigen of Salmonella enterica strain O53 were synthesized in good yield.
27308202	0	64	theme	Cell	71:74	arg1	O-Polysaccharide					81:96	the Cell Wall O-Polysaccharide	67:96	the Cell Wall O-Polysaccharide of Salmonella enterica O53	67:123	Convergent Synthesis of Oligosaccharide Fragments Corresponding to the Cell Wall O-Polysaccharide of Salmonella enterica O53.
27308202	3	65	theme	great	775:779	arg1	interest					781:788	great interest	775:788	great interest	775:788	The development of synthetic strategies to prepare glycoconjugate derivatives against pathogenic bacterial strains is therefore of great interest.
27308202	3	66	theme	synthetic	663:671	arg1	strategies					673:682	synthetic strategies to prepare glycoconjugate derivatives against pathogenic bacterial strains	663:757	synthetic strategies to prepare glycoconjugate derivatives against pathogenic bacterial strains	663:757	The development of synthetic strategies to prepare glycoconjugate derivatives against pathogenic bacterial strains is therefore of great interest.
27308202	1	67	with	variations	378:387	arg1	drawbacks					269:277	some significant drawbacks	252:277	some significant drawbacks such as handling of live bacterial strains	252:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	1	67	with	variations	378:387	arg1	handling					287:294	handling	287:294	handling of live bacterial strains	287:320	Conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure.
27308202	8	68	theme	reactions	1263:1271	arg1	outcomes					1233:1240	The stereoselective outcomes	1213:1240	The stereoselective outcomes of all glycosylation reactions	1213:1271	The stereoselective outcomes of all glycosylation reactions were very good.
27308202	8	68	theme	reactions	1263:1271	arg1	good					1283:1286	good	1283:1286	good	1283:1286	The stereoselective outcomes of all glycosylation reactions were very good.
26299711	1	0	theme	Cu2O	151:154	arg1	synthesis					138:146	in situ synthesis	130:146	in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O	130:310	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	3	1	theme	potential	640:648	arg1	zone					650:653	local high potential zone	629:653	local high potential zone	629:653	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	1	2	from	Cu2O	151:154	arg1	film					217:220	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	1	3	used	used	256:259	arg2	microreactor					268:279	the microreactor	264:279	the microreactor for the formation of nano Cu2O	264:310	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	1	3	used	used	256:259	arg2	film					247:250	the porous chitin film	229:250	the porous chitin film	229:250	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	1	4	dep	in	130:131	arg1	situ					133:136	situ	133:136	situ	133:136	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	4	5	theme	Cu2O	819:822	arg1	size					811:814	the size	807:814	the size of Cu2O	807:822	So it was found that the porous chitin film could load Cu2O and graphene at the same time, controlling the size of Cu2O and leading to easy recycle and reuse of the photocatalyst.
26299711	1	6	theme	nano	302:305	arg1	Cu2O					307:310	nano Cu2O	302:310	nano Cu2O	302:310	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	5	7	theme	potential	1017:1025	arg1	application					1027:1037	great potential application	1011:1037	great potential application	1011:1037	Moreover, the introduction of GO has dramatically improved the photocatalytic activity of Cu2O in the Cu2O/GO/RC film, showing great potential application in wastewater treatment utilizing solar energy.
26299711	1	8	from	synthesis	138:146	arg1	film					217:220	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	1	9	theme	Cu2O	307:310	arg1	formation					289:297	the formation	285:297	the formation of nano Cu2O	285:310	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	3	10	theme	local	629:633	arg1	zone					650:653	local high potential zone	629:653	local high potential zone	629:653	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	4	11	theme	chitin	736:741	arg1	film					743:746	the porous chitin film	725:746	the porous chitin film	725:746	So it was found that the porous chitin film could load Cu2O and graphene at the same time, controlling the size of Cu2O and leading to easy recycle and reuse of the photocatalyst.
26299711	5	12	theme	Cu2O	974:977	arg1	activity					962:969	the photocatalytic activity	943:969	the photocatalytic activity of Cu2O in the Cu2O/GO/RC film	943:1000	Moreover, the introduction of GO has dramatically improved the photocatalytic activity of Cu2O in the Cu2O/GO/RC film, showing great potential application in wastewater treatment utilizing solar energy.
26299711	1	13	theme	regenerated	163:173	arg1	oxide					196:200	regenerated chitin (RC)/graphene oxide	163:200	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	4	14	dep	load	754:757	arg1	leading					828:834	leading	828:834	leading to easy recycle and reuse of the photocatalyst	828:881	So it was found that the porous chitin film could load Cu2O and graphene at the same time, controlling the size of Cu2O and leading to easy recycle and reuse of the photocatalyst.
26299711	4	14	dep	load	754:757	arg1	controlling					795:805	controlling	795:805	controlling the size of Cu2O	795:822	So it was found that the porous chitin film could load Cu2O and graphene at the same time, controlling the size of Cu2O and leading to easy recycle and reuse of the photocatalyst.
26299711	3	15	theme	high	635:638	arg1	zone					650:653	local high potential zone	629:653	local high potential zone	629:653	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	5	16	theme	wastewater	1042:1051	arg1	treatment					1053:1061	wastewater treatment	1042:1061	wastewater treatment utilizing solar energy	1042:1084	Moreover, the introduction of GO has dramatically improved the photocatalytic activity of Cu2O in the Cu2O/GO/RC film, showing great potential application in wastewater treatment utilizing solar energy.
26299711	3	17	dep	inside	423:428	arg1	generate					452:459	generate	452:459	generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points	452:701	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	3	17	dep	inside	423:428	arg1	excite					441:446	excite	441:446	excite	441:446	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	3	18	theme	zone	650:653	arg1	generation					615:624	the generation	611:624	the generation of local high potential zone	611:653	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	1	19	theme	chitin	175:180	arg1	oxide					196:200	regenerated chitin (RC)/graphene oxide	163:200	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	0	20	theme	New	0:2	arg1	photocatalyst					4:16	New photocatalyst	0:16	New photocatalyst	0:16	New photocatalyst based on graphene oxide/chitin for degradation of dyes under sunlight.
26299711	1	21	theme	porous	233:238	arg1	film					247:250	the porous chitin film	229:250	the porous chitin film	229:250	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	1	21	theme	porous	233:238	arg1	microreactor					268:279	the microreactor	264:279	the microreactor for the formation of nano Cu2O	264:310	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	1	22	theme	Sunlight	89:96	arg1	photocatalyst					98:110	Sunlight photocatalyst	89:110	Sunlight photocatalyst	89:110	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	1	23	theme	chitin	240:245	arg1	film					247:250	the porous chitin film	229:250	the porous chitin film	229:250	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	1	23	theme	chitin	240:245	arg1	microreactor					268:279	the microreactor	264:279	the microreactor for the formation of nano Cu2O	264:310	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	1	24	theme	RC	183:184	arg1	oxide					196:200	regenerated chitin (RC)/graphene oxide	163:200	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	3	25	theme	electron	485:492	arg1	holes					494:498	electron holes	485:498	electron holes	485:498	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	5	26	theme	solar	1073:1077	arg1	energy					1079:1084	solar energy	1073:1084	solar energy	1073:1084	Moreover, the introduction of GO has dramatically improved the photocatalytic activity of Cu2O in the Cu2O/GO/RC film, showing great potential application in wastewater treatment utilizing solar energy.
26299711	1	27	from	film	217:220	arg1	synthesis					138:146	in situ synthesis	130:146	in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O	130:310	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	1	28	theme	/graphene	186:194	arg1	oxide					196:200	regenerated chitin (RC)/graphene oxide	163:200	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	0	29	theme	graphene	27:34	arg1	oxide/chitin					36:47	graphene oxide/chitin	27:47	graphene oxide/chitin for degradation of dyes under sunlight	27:86	New photocatalyst based on graphene oxide/chitin for degradation of dyes under sunlight.
26299711	3	30	theme	more	691:694	arg1	points					696:701	more points	691:701	more points	691:701	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	3	31	theme	dyes	546:549	arg1	degradation					531:541	the degradation	527:541	the degradation of dyes	527:549	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	5	32	theme	photocatalytic	947:960	arg1	activity					962:969	the photocatalytic activity	943:969	the photocatalytic activity of Cu2O in the Cu2O/GO/RC film	943:1000	Moreover, the introduction of GO has dramatically improved the photocatalytic activity of Cu2O in the Cu2O/GO/RC film, showing great potential application in wastewater treatment utilizing solar energy.
26299711	1	33	theme	oxide	196:200	arg1	film					217:220	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	5	34	theme	great	1011:1015	arg1	application					1027:1037	great potential application	1011:1037	great potential application	1011:1037	Moreover, the introduction of GO has dramatically improved the photocatalytic activity of Cu2O in the Cu2O/GO/RC film, showing great potential application in wastewater treatment utilizing solar energy.
26299711	2	35	dep	sheets	410:415	arg1	GO					407:408	GO	407:408	GO	407:408	Nano Cu2O was immobilized and evenly distributed in the matrix and Cu2O tended to grow on the GO sheets.
26299711	3	36	theme	chain	670:674	arg1	degradation					676:686	the chain degradation	666:686	the chain degradation	666:686	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	4	37	theme	porous	729:734	arg1	film					743:746	the porous chitin film	725:746	the porous chitin film	725:746	So it was found that the porous chitin film could load Cu2O and graphene at the same time, controlling the size of Cu2O and leading to easy recycle and reuse of the photocatalyst.
26299711	4	38	theme	photocatalyst	869:881	arg1	recycle					844:850	easy recycle	839:850	easy recycle	839:850	So it was found that the porous chitin film could load Cu2O and graphene at the same time, controlling the size of Cu2O and leading to easy recycle and reuse of the photocatalyst.
26299711	4	38	theme	photocatalyst	869:881	arg1	reuse					856:860	reuse	856:860	reuse of the photocatalyst	856:881	So it was found that the porous chitin film could load Cu2O and graphene at the same time, controlling the size of Cu2O and leading to easy recycle and reuse of the photocatalyst.
26299711	4	39	theme	same	784:787	arg1	time					789:792	the same time	780:792	the same time	780:792	So it was found that the porous chitin film could load Cu2O and graphene at the same time, controlling the size of Cu2O and leading to easy recycle and reuse of the photocatalyst.
26299711	5	40	theme	Cu2O/GO/RC	986:995	arg1	film					997:1000	the Cu2O/GO/RC film	982:1000	the Cu2O/GO/RC film	982:1000	Moreover, the introduction of GO has dramatically improved the photocatalytic activity of Cu2O in the Cu2O/GO/RC film, showing great potential application in wastewater treatment utilizing solar energy.
26299711	5	41	theme	GO	914:915	arg1	introduction					898:909	the introduction	894:909	the introduction of GO	894:915	Moreover, the introduction of GO has dramatically improved the photocatalytic activity of Cu2O in the Cu2O/GO/RC film, showing great potential application in wastewater treatment utilizing solar energy.
26299711	4	42	from	time	789:792	arg1	load					754:757	load	754:757	load	754:757	So it was found that the porous chitin film could load Cu2O and graphene at the same time, controlling the size of Cu2O and leading to easy recycle and reuse of the photocatalyst.
26299711	1	43	theme	in	130:131	arg1	synthesis					138:146	in situ synthesis	130:146	in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O	130:310	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	1	44	dep	oxide	196:200	arg1	GO					203:204	GO	203:204	GO	203:204	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
26299711	5	45	from	activity	962:969	arg1	film					997:1000	the Cu2O/GO/RC film	982:1000	the Cu2O/GO/RC film	982:1000	Moreover, the introduction of GO has dramatically improved the photocatalytic activity of Cu2O in the Cu2O/GO/RC film, showing great potential application in wastewater treatment utilizing solar energy.
26299711	0	46	theme	dyes	68:71	arg1	degradation					53:63	degradation	53:63	degradation of dyes under sunlight	53:86	New photocatalyst based on graphene oxide/chitin for degradation of dyes under sunlight.
26299711	3	47	theme	yielded	577:583	arg1	photoelectrons					585:598	the yielded photoelectrons	573:598	the yielded photoelectrons	573:598	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	2	48	theme	Nano	313:316	arg1	Cu2O					318:321	Nano Cu2O	313:321	Nano Cu2O	313:321	Nano Cu2O was immobilized and evenly distributed in the matrix and Cu2O tended to grow on the GO sheets.
26299711	3	49	dep	GO	558:559	arg1	transferred					561:571	transferred	561:571	GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points	558:701	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	4	50	theme	easy	839:842	arg1	recycle					844:850	easy recycle	839:850	easy recycle	839:850	So it was found that the porous chitin film could load Cu2O and graphene at the same time, controlling the size of Cu2O and leading to easy recycle and reuse of the photocatalyst.
26299711	3	51	theme	free	461:464	arg1	photoelectrons					466:479	free photoelectrons	461:479	free photoelectrons	461:479	Cu2O inside the matrix excite and generate free photoelectrons and electron holes, which was responsible for the degradation of dyes, while GO transferred the yielded photoelectrons to prevent the generation of local high potential zone and induce the chain degradation at more points.
26299711	1	52	theme	composite	207:215	arg1	film					217:220	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	the regenerated chitin (RC)/graphene oxide (GO) composite film	159:220	Sunlight photocatalyst was fabricated by in situ synthesis of Cu2O in the regenerated chitin (RC)/graphene oxide (GO) composite film, where the porous chitin film was used as the microreactor for the formation of nano Cu2O.
24365707	3	0	theme	limited	361:367	arg1	interaction					380:390	limited biological interaction	361:390	limited biological interaction	361:390	However, these composites have limited biological interaction, as they are often composed of synthetic polymers or non-ideal biological polymers, which lack the required elements for biorecognition.
24365707	5	1	theme	vinyl	691:695	arg1	-heparin					705:712	the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin	657:712	the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin	657:712	The CH was formed from the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin and the conductive polymer (CP), poly(3,4-ethylene dioxythiophene).
24365707	15	2	theme	flexible	1796:1803	arg1	platform					1816:1823	a flexible technology platform	1794:1823	a flexible technology platform	1794:1823	The CH system is a flexible technology platform, which can be tailored to covalently incorporate bioactive protein sequences and deliver mobile water-soluble drug molecules.
24365707	15	2	theme	flexible	1796:1803	arg1	system					1784:1789	The CH system	1777:1789	The CH system	1777:1789	The CH system is a flexible technology platform, which can be tailored to covalently incorporate bioactive protein sequences and deliver mobile water-soluble drug molecules.
24365707	3	3	theme	biological	369:378	arg1	interaction					380:390	limited biological interaction	361:390	limited biological interaction	361:390	However, these composites have limited biological interaction, as they are often composed of synthetic polymers or non-ideal biological polymers, which lack the required elements for biorecognition.
24365707	5	4	theme	alcohol	697:703	arg1	-heparin					705:712	the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin	657:712	the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin	657:712	The CH was formed from the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin and the conductive polymer (CP), poly(3,4-ethylene dioxythiophene).
24365707	6	5	theme	methacrylate	857:868	arg1	crosslinking					870:881	methacrylate crosslinking	857:881	methacrylate crosslinking within the CH	857:895	Adhesive biomolecules sericin and gelatin were covalently incorporated via methacrylate crosslinking within the CH.
24365707	6	6	dep	biomolecules	791:802	arg1	gelatin					816:822	gelatin	816:822	gelatin	816:822	Adhesive biomolecules sericin and gelatin were covalently incorporated via methacrylate crosslinking within the CH.
24365707	6	6	dep	biomolecules	791:802	arg1	sericin					804:810	sericin	804:810	sericin	804:810	Adhesive biomolecules sericin and gelatin were covalently incorporated via methacrylate crosslinking within the CH.
24365707	6	6	dep	biomolecules	791:802	arg1	biomolecules					791:802	Adhesive biomolecules	782:802	Adhesive biomolecules sericin and gelatin	782:822	Adhesive biomolecules sericin and gelatin were covalently incorporated via methacrylate crosslinking within the CH.
24365707	2	7	dep	Approaches	187:196	arg1	achieve					201:207	achieve	201:207	to achieve both electrical and mechanical improvements	198:251	Approaches to achieve both electrical and mechanical improvements have included the development of hydrogel and conducting polymer composites.
24365707	11	8	theme	best	1443:1446	arg1	support					1448:1454	the best support	1439:1454	the best support for the outgrowth of neural processes	1439:1492	Sericin provided the best support for the outgrowth of neural processes, and 1 wt.
24365707	7	9	theme	charge	1009:1014	arg1	transfer					1016:1023	charge transfer	1009:1023	charge transfer	1009:1023	Electrical properties of the bioactive CH were assessed, and it was shown that the polar biomolecules improved charge transfer.
24365707	7	10	theme	bioactive	927:935	arg1	CH					937:938	the bioactive CH	923:938	the bioactive CH	923:938	Electrical properties of the bioactive CH were assessed, and it was shown that the polar biomolecules improved charge transfer.
24365707	8	11	theme	CP	1214:1215	arg1	electropolymerization					1185:1205	electropolymerization	1185:1205	electropolymerization of the CP through the hydrogel	1185:1236	The bioactivity of heparin within the hybrid assessed by examining stimulation of B-lymphocyte (BaF3) proliferation showed that bioactivity was retained after electropolymerization of the CP through the hydrogel.
24365707	9	12	theme	small	1356:1360	arg1	percentages					1362:1372	only small percentages	1351:1372	only small percentages (⩽ 2 wt	1351:1380	Similarly, incorporation of sericin and gelatin in the CH promoted neural cell adhesion and proliferation, with only small percentages (⩽ 2 wt.
24365707	7	13	theme	polar	981:985	arg1	biomolecules					987:998	the polar biomolecules	977:998	the polar biomolecules	977:998	Electrical properties of the bioactive CH were assessed, and it was shown that the polar biomolecules improved charge transfer.
24365707	15	14	theme	CH	1781:1782	arg1	platform					1816:1823	a flexible technology platform	1794:1823	a flexible technology platform	1794:1823	The CH system is a flexible technology platform, which can be tailored to covalently incorporate bioactive protein sequences and deliver mobile water-soluble drug molecules.
24365707	15	14	theme	CH	1781:1782	arg1	system					1784:1789	The CH system	1777:1789	The CH system	1777:1789	The CH system is a flexible technology platform, which can be tailored to covalently incorporate bioactive protein sequences and deliver mobile water-soluble drug molecules.
24365707	13	15	theme	drug	1580:1583	arg1	capability					1594:1603	The drug delivery capability	1576:1603	The drug delivery capability of CH	1576:1609	The drug delivery capability of CH was shown through incorporation of nerve growth factor during polymer fabrication.
24365707	15	16	theme	technology	1805:1814	arg1	platform					1816:1823	a flexible technology platform	1794:1823	a flexible technology platform	1794:1823	The CH system is a flexible technology platform, which can be tailored to covalently incorporate bioactive protein sequences and deliver mobile water-soluble drug molecules.
24365707	15	16	theme	technology	1805:1814	arg1	system					1784:1789	The CH system	1777:1789	The CH system	1777:1789	The CH system is a flexible technology platform, which can be tailored to covalently incorporate bioactive protein sequences and deliver mobile water-soluble drug molecules.
24365707	5	17	theme	3,4-ethylene	752:763	arg1	poly					747:750	poly	747:750	poly(3,4-ethylene dioxythiophene)	747:779	The CH was formed from the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin and the conductive polymer (CP), poly(3,4-ethylene dioxythiophene).
24365707	5	17	theme	3,4-ethylene	752:763	arg1	dioxythiophene					765:778	3,4-ethylene dioxythiophene	752:778	3,4-ethylene dioxythiophene	752:778	The CH was formed from the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin and the conductive polymer (CP), poly(3,4-ethylene dioxythiophene).
24365707	5	18	theme	biosynthetic	661:672	arg1	-heparin					705:712	the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin	657:712	the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin	657:712	The CH was formed from the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin and the conductive polymer (CP), poly(3,4-ethylene dioxythiophene).
24365707	10	19	theme	optimal	1405:1411	arg1	results					1413:1419	optimal results	1405:1419	optimal results	1405:1419	%) required to achieve optimal results.
24365707	2	20	theme	composites	318:327	arg1	development					271:281	the development	267:281	the development of hydrogel and conducting polymer composites	267:327	Approaches to achieve both electrical and mechanical improvements have included the development of hydrogel and conducting polymer composites.
24365707	0	21	theme	implantable	51:61	arg1	coatings					73:80	implantable electrode coatings	51:80	implantable electrode coatings	51:80	Conductive hydrogels with tailored bioactivity for implantable electrode coatings.
24365707	5	22	theme	co-hydrogel	674:684	arg1	-heparin					705:712	the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin	657:712	the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin	657:712	The CH was formed from the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin and the conductive polymer (CP), poly(3,4-ethylene dioxythiophene).
24365707	8	23	theme	heparin	1045:1051	arg1	bioactivity					1030:1040	The bioactivity	1026:1040	The bioactivity of heparin within the hybrid assessed by examining stimulation of B-lymphocyte (BaF3) proliferation	1026:1140	The bioactivity of heparin within the hybrid assessed by examining stimulation of B-lymphocyte (BaF3) proliferation showed that bioactivity was retained after electropolymerization of the CP through the hydrogel.
24365707	2	24	theme	polymer	310:316	arg1	composites					318:327	hydrogel and conducting polymer composites	286:327	composites	318:327	Approaches to achieve both electrical and mechanical improvements have included the development of hydrogel and conducting polymer composites.
24365707	3	25	contain	have	356:359	arg1	composites					345:354	these composites	339:354	these composites	339:354	However, these composites have limited biological interaction, as they are often composed of synthetic polymers or non-ideal biological polymers, which lack the required elements for biorecognition.
24365707	3	25	contain	have	356:359	arg2	interaction					380:390	limited biological interaction	361:390	limited biological interaction	361:390	However, these composites have limited biological interaction, as they are often composed of synthetic polymers or non-ideal biological polymers, which lack the required elements for biorecognition.
24365707	3	26	theme	synthetic	423:431	arg1	polymers					433:440	synthetic polymers	423:440	synthetic polymers	423:440	However, these composites have limited biological interaction, as they are often composed of synthetic polymers or non-ideal biological polymers, which lack the required elements for biorecognition.
24365707	9	27	theme	⩽	1375:1375	arg1	wt					1379:1380	⩽ 2 wt	1375:1380	⩽ 2 wt	1375:1380	Similarly, incorporation of sericin and gelatin in the CH promoted neural cell adhesion and proliferation, with only small percentages (⩽ 2 wt.
24365707	5	28	theme	poly	686:689	arg1	-heparin					705:712	the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin	657:712	the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin	657:712	The CH was formed from the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin and the conductive polymer (CP), poly(3,4-ethylene dioxythiophene).
24365707	9	29	theme	sericin	1267:1273	arg1	incorporation					1250:1262	incorporation	1250:1262	incorporation of sericin and gelatin in the CH	1250:1295	Similarly, incorporation of sericin and gelatin in the CH promoted neural cell adhesion and proliferation, with only small percentages (⩽ 2 wt.
24365707	13	30	theme	delivery	1585:1592	arg1	capability					1594:1603	The drug delivery capability	1576:1603	The drug delivery capability of CH	1576:1609	The drug delivery capability of CH was shown through incorporation of nerve growth factor during polymer fabrication.
24365707	7	31	theme	Electrical	898:907	arg1	properties					909:918	Electrical properties	898:918	Electrical properties of the bioactive CH	898:938	Electrical properties of the bioactive CH were assessed, and it was shown that the polar biomolecules improved charge transfer.
24365707	0	32	theme	Conductive	0:9	arg1	hydrogels					11:19	Conductive hydrogels	0:19	Conductive hydrogels with tailored bioactivity for implantable electrode coatings	0:80	Conductive hydrogels with tailored bioactivity for implantable electrode coatings.
24365707	8	33	theme	B-lymphocyte	1108:1119	arg1	proliferation					1128:1140	B-lymphocyte (BaF3) proliferation	1108:1140	B-lymphocyte (BaF3) proliferation	1108:1140	The bioactivity of heparin within the hybrid assessed by examining stimulation of B-lymphocyte (BaF3) proliferation showed that bioactivity was retained after electropolymerization of the CP through the hydrogel.
24365707	15	34	theme	drug	1935:1938	arg1	molecules					1940:1948	mobile water-soluble drug molecules	1914:1948	mobile water-soluble drug molecules	1914:1948	The CH system is a flexible technology platform, which can be tailored to covalently incorporate bioactive protein sequences and deliver mobile water-soluble drug molecules.
24365707	1	35	theme	high-resolution	102:116	arg1	neuroprosthetics					118:133	high-resolution neuroprosthetics	102:133	high-resolution neuroprosthetics	102:133	The development of high-resolution neuroprosthetics has driven the need for better electrode materials.
24365707	0	36	theme	tailored	26:33	arg1	bioactivity					35:45	tailored bioactivity	26:45	tailored bioactivity for implantable electrode coatings	26:80	Conductive hydrogels with tailored bioactivity for implantable electrode coatings.
24365707	4	37	theme	conducting	608:617	arg1	hydrogel					619:626	a conducting hydrogel	606:626	a conducting hydrogel (CH)	606:631	This study explores the covalent incorporation of bioactive molecules within a conducting hydrogel (CH).
24365707	4	37	theme	conducting	608:617	arg1	CH					629:630	CH	629:630	CH	629:630	This study explores the covalent incorporation of bioactive molecules within a conducting hydrogel (CH).
24365707	9	38	theme	gelatin	1279:1285	arg1	incorporation					1250:1262	incorporation	1250:1262	incorporation of sericin and gelatin in the CH	1250:1295	Similarly, incorporation of sericin and gelatin in the CH promoted neural cell adhesion and proliferation, with only small percentages (⩽ 2 wt.
24365707	1	39	theme	neuroprosthetics	118:133	arg1	development					87:97	The development	83:97	The development of high-resolution neuroprosthetics	83:133	The development of high-resolution neuroprosthetics has driven the need for better electrode materials.
24365707	15	40	theme	water-soluble	1921:1933	arg1	molecules					1940:1948	mobile water-soluble drug molecules	1914:1948	mobile water-soluble drug molecules	1914:1948	The CH system is a flexible technology platform, which can be tailored to covalently incorporate bioactive protein sequences and deliver mobile water-soluble drug molecules.
24365707	8	41	theme	BaF3	1122:1125	arg1	proliferation					1128:1140	B-lymphocyte (BaF3) proliferation	1108:1140	B-lymphocyte (BaF3) proliferation	1108:1140	The bioactivity of heparin within the hybrid assessed by examining stimulation of B-lymphocyte (BaF3) proliferation showed that bioactivity was retained after electropolymerization of the CP through the hydrogel.
24365707	13	42	theme	polymer	1673:1679	arg1	fabrication					1681:1691	polymer fabrication	1673:1691	polymer fabrication	1673:1691	The drug delivery capability of CH was shown through incorporation of nerve growth factor during polymer fabrication.
24365707	13	43	theme	CH	1608:1609	arg1	capability					1594:1603	The drug delivery capability	1576:1603	The drug delivery capability of CH	1576:1609	The drug delivery capability of CH was shown through incorporation of nerve growth factor during polymer fabrication.
24365707	11	44	theme	neural	1477:1482	arg1	processes					1484:1492	neural processes	1477:1492	neural processes	1477:1492	Sericin provided the best support for the outgrowth of neural processes, and 1 wt.
24365707	13	45	theme	growth	1652:1657	arg1	factor					1659:1664	nerve growth factor	1646:1664	nerve growth factor	1646:1664	The drug delivery capability of CH was shown through incorporation of nerve growth factor during polymer fabrication.
24365707	3	46	theme	biological	455:464	arg1	polymers					466:473	non-ideal biological polymers	445:473	non-ideal biological polymers	445:473	However, these composites have limited biological interaction, as they are often composed of synthetic polymers or non-ideal biological polymers, which lack the required elements for biorecognition.
24365707	11	47	theme	processes	1484:1492	arg1	outgrowth					1464:1472	the outgrowth	1460:1472	the outgrowth of neural processes	1460:1492	Sericin provided the best support for the outgrowth of neural processes, and 1 wt.
24365707	3	48	theme	required	491:498	arg1	elements					500:507	the required elements	487:507	the required elements for biorecognition	487:526	However, these composites have limited biological interaction, as they are often composed of synthetic polymers or non-ideal biological polymers, which lack the required elements for biorecognition.
24365707	4	49	theme	molecules	589:597	arg1	incorporation					562:574	the covalent incorporation	549:574	the covalent incorporation of bioactive molecules	549:597	This study explores the covalent incorporation of bioactive molecules within a conducting hydrogel (CH).
24365707	8	50	theme	proliferation	1128:1140	arg1	stimulation					1093:1103	stimulation	1093:1103	stimulation of B-lymphocyte (BaF3) proliferation	1093:1140	The bioactivity of heparin within the hybrid assessed by examining stimulation of B-lymphocyte (BaF3) proliferation showed that bioactivity was retained after electropolymerization of the CP through the hydrogel.
24365707	14	51	theme	target	1719:1724	arg1	cells					1726:1730	the target cells	1715:1730	the target cells	1715:1730	NGF was delivered to the target cells, resulting in outgrowth of neural processes.
24365707	15	52	theme	mobile	1914:1919	arg1	molecules					1940:1948	mobile water-soluble drug molecules	1914:1948	mobile water-soluble drug molecules	1914:1948	The CH system is a flexible technology platform, which can be tailored to covalently incorporate bioactive protein sequences and deliver mobile water-soluble drug molecules.
24365707	0	53	theme	electrode	63:71	arg1	coatings					73:80	implantable electrode coatings	51:80	implantable electrode coatings	51:80	Conductive hydrogels with tailored bioactivity for implantable electrode coatings.
24365707	4	54	theme	bioactive	579:587	arg1	molecules					589:597	bioactive molecules	579:597	bioactive molecules	579:597	This study explores the covalent incorporation of bioactive molecules within a conducting hydrogel (CH).
24365707	15	55	theme	bioactive	1874:1882	arg1	sequences					1892:1900	bioactive protein sequences	1874:1900	bioactive protein sequences	1874:1900	The CH system is a flexible technology platform, which can be tailored to covalently incorporate bioactive protein sequences and deliver mobile water-soluble drug molecules.
24365707	6	56	theme	Adhesive	782:789	arg1	gelatin					816:822	gelatin	816:822	gelatin	816:822	Adhesive biomolecules sericin and gelatin were covalently incorporated via methacrylate crosslinking within the CH.
24365707	6	56	theme	Adhesive	782:789	arg1	sericin					804:810	sericin	804:810	sericin	804:810	Adhesive biomolecules sericin and gelatin were covalently incorporated via methacrylate crosslinking within the CH.
24365707	6	56	theme	Adhesive	782:789	arg1	biomolecules					791:802	Adhesive biomolecules	782:802	Adhesive biomolecules sericin and gelatin	782:822	Adhesive biomolecules sericin and gelatin were covalently incorporated via methacrylate crosslinking within the CH.
24365707	3	57	theme	non-ideal	445:453	arg1	polymers					466:473	non-ideal biological polymers	445:473	non-ideal biological polymers	445:473	However, these composites have limited biological interaction, as they are often composed of synthetic polymers or non-ideal biological polymers, which lack the required elements for biorecognition.
24365707	13	58	theme	nerve	1646:1650	arg1	factor					1659:1664	nerve growth factor	1646:1664	nerve growth factor	1646:1664	The drug delivery capability of CH was shown through incorporation of nerve growth factor during polymer fabrication.
24365707	9	59	theme	neural	1306:1311	arg1	adhesion					1318:1325	neural cell adhesion	1306:1325	neural cell adhesion	1306:1325	Similarly, incorporation of sericin and gelatin in the CH promoted neural cell adhesion and proliferation, with only small percentages (⩽ 2 wt.
24365707	9	60	theme	cell	1313:1316	arg1	adhesion					1318:1325	neural cell adhesion	1306:1325	neural cell adhesion	1306:1325	Similarly, incorporation of sericin and gelatin in the CH promoted neural cell adhesion and proliferation, with only small percentages (⩽ 2 wt.
24365707	0	61	with	hydrogels	11:19	arg1	bioactivity					35:45	tailored bioactivity	26:45	tailored bioactivity for implantable electrode coatings	26:80	Conductive hydrogels with tailored bioactivity for implantable electrode coatings.
24365707	9	62	dep	percentages	1362:1372	arg1	wt					1379:1380	⩽ 2 wt	1375:1380	⩽ 2 wt	1375:1380	Similarly, incorporation of sericin and gelatin in the CH promoted neural cell adhesion and proliferation, with only small percentages (⩽ 2 wt.
24365707	5	63	theme	conductive	722:731	arg1	CP					742:743	CP	742:743	CP	742:743	The CH was formed from the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin and the conductive polymer (CP), poly(3,4-ethylene dioxythiophene).
24365707	5	63	theme	conductive	722:731	arg1	poly					747:750	poly	747:750	poly(3,4-ethylene dioxythiophene)	747:779	The CH was formed from the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin and the conductive polymer (CP), poly(3,4-ethylene dioxythiophene).
24365707	5	63	theme	conductive	722:731	arg1	polymer					733:739	the conductive polymer	718:739	the conductive polymer (CP)	718:744	The CH was formed from the biosynthetic co-hydrogel poly(vinyl alcohol)-heparin and the conductive polymer (CP), poly(3,4-ethylene dioxythiophene).
24365707	2	64	theme	mechanical	229:238	arg1	improvements					240:251	both electrical and mechanical improvements	209:251	both electrical and mechanical improvements	209:251	Approaches to achieve both electrical and mechanical improvements have included the development of hydrogel and conducting polymer composites.
24365707	2	65	theme	conducting	299:308	arg1	composites					318:327	hydrogel and conducting polymer composites	286:327	composites	318:327	Approaches to achieve both electrical and mechanical improvements have included the development of hydrogel and conducting polymer composites.
24365707	13	66	theme	factor	1659:1664	arg1	incorporation					1629:1641	incorporation	1629:1641	incorporation of nerve growth factor during polymer fabrication	1629:1691	The drug delivery capability of CH was shown through incorporation of nerve growth factor during polymer fabrication.
24365707	9	67	from	incorporation	1250:1262	arg1	CH					1294:1295	the CH	1290:1295	the CH	1290:1295	Similarly, incorporation of sericin and gelatin in the CH promoted neural cell adhesion and proliferation, with only small percentages (⩽ 2 wt.
24365707	12	68	theme	neurons	1567:1573	arg1	differentiation					1548:1562	differentiation	1548:1562	differentiation	1548:1562	% was sufficient to facilitate adhesion and differentiation of neurons.
24365707	12	68	theme	neurons	1567:1573	arg1	adhesion					1535:1542	adhesion	1535:1542	adhesion	1535:1542	% was sufficient to facilitate adhesion and differentiation of neurons.
24365707	2	69	theme	electrical	214:223	arg1	improvements					240:251	both electrical and mechanical improvements	209:251	both electrical and mechanical improvements	209:251	Approaches to achieve both electrical and mechanical improvements have included the development of hydrogel and conducting polymer composites.
24365707	2	70	theme	hydrogel	286:293	arg1	composites					318:327	hydrogel and conducting polymer composites	286:327	composites	318:327	Approaches to achieve both electrical and mechanical improvements have included the development of hydrogel and conducting polymer composites.
24365707	1	71	theme	better	159:164	arg1	materials					176:184	better electrode materials	159:184	better electrode materials	159:184	The development of high-resolution neuroprosthetics has driven the need for better electrode materials.
24365707	14	72	theme	processes	1766:1774	arg1	outgrowth					1746:1754	outgrowth	1746:1754	outgrowth of neural processes	1746:1774	NGF was delivered to the target cells, resulting in outgrowth of neural processes.
24365707	15	73	theme	protein	1884:1890	arg1	sequences					1892:1900	bioactive protein sequences	1874:1900	bioactive protein sequences	1874:1900	The CH system is a flexible technology platform, which can be tailored to covalently incorporate bioactive protein sequences and deliver mobile water-soluble drug molecules.
24365707	4	74	theme	covalent	553:560	arg1	incorporation					562:574	the covalent incorporation	549:574	the covalent incorporation of bioactive molecules	549:597	This study explores the covalent incorporation of bioactive molecules within a conducting hydrogel (CH).
24365707	1	75	theme	electrode	166:174	arg1	materials					176:184	better electrode materials	159:184	better electrode materials	159:184	The development of high-resolution neuroprosthetics has driven the need for better electrode materials.
24365707	7	76	theme	CH	937:938	arg1	properties					909:918	Electrical properties	898:918	Electrical properties of the bioactive CH	898:938	Electrical properties of the bioactive CH were assessed, and it was shown that the polar biomolecules improved charge transfer.
24365707	14	77	theme	neural	1759:1764	arg1	processes					1766:1774	neural processes	1759:1774	neural processes	1759:1774	NGF was delivered to the target cells, resulting in outgrowth of neural processes.
24333423	0	0	theme	reconstituted	76:88	arg1	synthase					101:108	reconstituted hyaluronan synthase	76:108	reconstituted hyaluronan synthase	76:108	Effect of a cholesterol-rich lipid environment on the enzymatic activity of reconstituted hyaluronan synthase.
24333423	1	1	theme	unique	142:147	arg1	glycosyltransferase					169:187	a unique membrane-associated glycosyltransferase	140:187	a unique membrane-associated glycosyltransferase	140:187	Hyaluronan synthase (HAS) is a unique membrane-associated glycosyltransferase and its activity is lipid dependent.
24333423	1	1	theme	unique	142:147	arg1	synthase					122:129	Hyaluronan synthase	111:129	Hyaluronan synthase (HAS)	111:135	Hyaluronan synthase (HAS) is a unique membrane-associated glycosyltransferase and its activity is lipid dependent.
24333423	7	2	theme	recombinant	954:964	arg1	proteins					971:978	the recombinant HAS2 proteins	950:978	the recombinant HAS2 proteins	950:978	When the recombinant HAS2 proteins were reconstituted into liposomes composed of both saturated phosphatidylcholine and cholesterol, this provided a higher enzyme activity as compared with the liposomes formed by phosphatidylcholine alone.
24333423	3	3	theme	functional	332:341	arg1	association					343:353	the functional association	328:353	the functional association of hyaluronan synthesis in a cholesterol-rich membrane-environment	328:420	Here we investigated the functional association of hyaluronan synthesis in a cholesterol-rich membrane-environment.
24333423	5	4	theme	the	669:671	arg1	activity					677:684	the HAS activity	669:684	the HAS activity	669:684	The sequestration of cellular cholesterol by methyl-ß-cyclodextrin also decreased the hyaluronan production of fibroblasts, as well as the HAS activity.
24333423	1	5	theme	membrane-associated	149:167	arg1	glycosyltransferase					169:187	a unique membrane-associated glycosyltransferase	140:187	a unique membrane-associated glycosyltransferase	140:187	Hyaluronan synthase (HAS) is a unique membrane-associated glycosyltransferase and its activity is lipid dependent.
24333423	1	5	theme	membrane-associated	149:167	arg1	synthase					122:129	Hyaluronan synthase	111:129	Hyaluronan synthase (HAS)	111:135	Hyaluronan synthase (HAS) is a unique membrane-associated glycosyltransferase and its activity is lipid dependent.
24333423	0	6	theme	synthase	101:108	arg1	activity					64:71	the enzymatic activity	50:71	the enzymatic activity of reconstituted hyaluronan synthase	50:108	Effect of a cholesterol-rich lipid environment on the enzymatic activity of reconstituted hyaluronan synthase.
24333423	0	7	from	Effect	0:5	arg1	activity					64:71	the enzymatic activity	50:71	the enzymatic activity of reconstituted hyaluronan synthase	50:108	Effect of a cholesterol-rich lipid environment on the enzymatic activity of reconstituted hyaluronan synthase.
24333423	11	8	theme	cholesterol-dependent	1667:1687	arg1	alterations					1689:1699	cholesterol-dependent alterations	1667:1699	cholesterol-dependent alterations	1667:1699	Taken together, the present data suggests a clear functional association between HAS activity and cholesterol-dependent alterations in the physical and chemical properties of cell membranes.
24333423	0	9	theme	hyaluronan	90:99	arg1	synthase					101:108	reconstituted hyaluronan synthase	76:108	reconstituted hyaluronan synthase	76:108	Effect of a cholesterol-rich lipid environment on the enzymatic activity of reconstituted hyaluronan synthase.
24333423	9	10	theme	different	1351:1359	arg1	compositions					1367:1378	different lipid compositions	1351:1378	different lipid compositions	1351:1378	Furthermore, the activation profiles of different lipid compositions were determined in the presence or absence of cholesterol.
24333423	4	11	theme	dermal	444:449	arg1	fibroblasts					451:461	human dermal fibroblasts	438:461	human dermal fibroblasts	438:461	The culture of human dermal fibroblasts in lipoprotein-depleted medium attenuated the synthesis of hyaluronan.
24333423	2	12	theme	vertebrate	287:296	arg1	systems					298:304	vertebrate systems	287:304	vertebrate systems	287:304	The dependence however is not well understood, especially in vertebrate systems.
24333423	3	13	theme	hyaluronan	358:367	arg1	synthesis					369:377	hyaluronan synthesis	358:377	hyaluronan synthesis	358:377	Here we investigated the functional association of hyaluronan synthesis in a cholesterol-rich membrane-environment.
24333423	4	14	theme	hyaluronan	522:531	arg1	synthesis					509:517	the synthesis	505:517	the synthesis of hyaluronan	505:531	The culture of human dermal fibroblasts in lipoprotein-depleted medium attenuated the synthesis of hyaluronan.
24333423	4	15	theme	human	438:442	arg1	fibroblasts					451:461	human dermal fibroblasts	438:461	human dermal fibroblasts	438:461	The culture of human dermal fibroblasts in lipoprotein-depleted medium attenuated the synthesis of hyaluronan.
24333423	1	16	dep	synthase	122:129	arg1	HAS					132:134	HAS	132:134	HAS	132:134	Hyaluronan synthase (HAS) is a unique membrane-associated glycosyltransferase and its activity is lipid dependent.
24333423	5	17	theme	cellular	555:562	arg1	cholesterol					564:574	cellular cholesterol	555:574	cellular cholesterol	555:574	The sequestration of cellular cholesterol by methyl-ß-cyclodextrin also decreased the hyaluronan production of fibroblasts, as well as the HAS activity.
24333423	3	18	theme	synthesis	369:377	arg1	association					343:353	the functional association	328:353	the functional association of hyaluronan synthesis in a cholesterol-rich membrane-environment	328:420	Here we investigated the functional association of hyaluronan synthesis in a cholesterol-rich membrane-environment.
24333423	7	19	theme	enzyme	1101:1106	arg1	activity					1108:1115	a higher enzyme activity	1092:1115	a higher enzyme activity	1092:1115	When the recombinant HAS2 proteins were reconstituted into liposomes composed of both saturated phosphatidylcholine and cholesterol, this provided a higher enzyme activity as compared with the liposomes formed by phosphatidylcholine alone.
24333423	11	20	theme	chemical	1721:1728	arg1	properties					1730:1739	the physical and chemical properties	1704:1739	the physical and chemical properties of cell membranes	1704:1757	Taken together, the present data suggests a clear functional association between HAS activity and cholesterol-dependent alterations in the physical and chemical properties of cell membranes.
24333423	4	21	from	culture	427:433	arg1	medium					487:492	lipoprotein-depleted medium	466:492	lipoprotein-depleted medium	466:492	The culture of human dermal fibroblasts in lipoprotein-depleted medium attenuated the synthesis of hyaluronan.
24333423	5	22	theme	cholesterol	564:574	arg1	sequestration					538:550	The sequestration	534:550	The sequestration of cellular cholesterol by methyl-ß-cyclodextrin	534:599	The sequestration of cellular cholesterol by methyl-ß-cyclodextrin also decreased the hyaluronan production of fibroblasts, as well as the HAS activity.
24333423	8	23	theme	phosphatidylcholine	1275:1293	arg1	ratio					1266:1270	the molar ratio	1256:1270	the molar ratio of phosphatidylcholine to cholesterol	1256:1308	Cholesterol regulates HAS2 activity in a biphasic manner, depending on the molar ratio of phosphatidylcholine to cholesterol.
24333423	6	24	theme	recombinant	754:764	arg1	protein					834:840	a membrane protein	823:840	a membrane protein	823:840	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	6	24	theme	recombinant	754:764	arg1	protein					777:783	a recombinant human HAS2 protein	752:783	a recombinant human HAS2 protein with a histidine-tag	752:804	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	9	25	dep	presence	1403:1410	arg1	the					1399:1401	the	1399:1401	the	1399:1401	Furthermore, the activation profiles of different lipid compositions were determined in the presence or absence of cholesterol.
24333423	11	26	theme	physical	1708:1715	arg1	properties					1730:1739	the physical and chemical properties	1704:1739	the physical and chemical properties of cell membranes	1704:1757	Taken together, the present data suggests a clear functional association between HAS activity and cholesterol-dependent alterations in the physical and chemical properties of cell membranes.
24333423	7	27	theme	HAS2	966:969	arg1	proteins					971:978	the recombinant HAS2 proteins	950:978	the recombinant HAS2 proteins	950:978	When the recombinant HAS2 proteins were reconstituted into liposomes composed of both saturated phosphatidylcholine and cholesterol, this provided a higher enzyme activity as compared with the liposomes formed by phosphatidylcholine alone.
24333423	3	28	theme	cholesterol-rich	384:399	arg1	membrane-environment					401:420	a cholesterol-rich membrane-environment	382:420	a cholesterol-rich membrane-environment	382:420	Here we investigated the functional association of hyaluronan synthesis in a cholesterol-rich membrane-environment.
24333423	10	29	contain	had	1451:1453	arg1	Cholesterol					1439:1449	Cholesterol	1439:1449	Cholesterol	1439:1449	Cholesterol had the opposite effect on the HAS2 activity in liposomes composed of phosphatidylethanolamine or phosphatidylserine.
24333423	10	29	contain	had	1451:1453	arg2	effect					1468:1473	the opposite effect	1455:1473	the opposite effect	1455:1473	Cholesterol had the opposite effect on the HAS2 activity in liposomes composed of phosphatidylethanolamine or phosphatidylserine.
24333423	6	30	theme	baculovirus	853:863	arg1	system					865:870	a baculovirus system	851:870	a baculovirus system	851:870	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	9	31	theme	activation	1328:1337	arg1	profiles					1339:1346	the activation profiles	1324:1346	the activation profiles of different lipid compositions	1324:1378	Furthermore, the activation profiles of different lipid compositions were determined in the presence or absence of cholesterol.
24333423	1	32	theme	Hyaluronan	111:120	arg1	synthase					122:129	Hyaluronan synthase	111:129	Hyaluronan synthase (HAS)	111:135	Hyaluronan synthase (HAS) is a unique membrane-associated glycosyltransferase and its activity is lipid dependent.
24333423	1	32	theme	Hyaluronan	111:120	arg1	glycosyltransferase					169:187	a unique membrane-associated glycosyltransferase	140:187	a unique membrane-associated glycosyltransferase	140:187	Hyaluronan synthase (HAS) is a unique membrane-associated glycosyltransferase and its activity is lipid dependent.
24333423	7	33	theme	saturated	1031:1039	arg1	phosphatidylcholine					1041:1059	saturated phosphatidylcholine	1031:1059	saturated phosphatidylcholine	1031:1059	When the recombinant HAS2 proteins were reconstituted into liposomes composed of both saturated phosphatidylcholine and cholesterol, this provided a higher enzyme activity as compared with the liposomes formed by phosphatidylcholine alone.
24333423	0	34	theme	environment	35:45	arg1	Effect					0:5	Effect	0:5	Effect of a cholesterol-rich lipid environment on the enzymatic activity of reconstituted hyaluronan synthase.	0:109	Effect of a cholesterol-rich lipid environment on the enzymatic activity of reconstituted hyaluronan synthase.
24333423	6	35	theme	HAS2	772:775	arg1	protein					834:840	a membrane protein	823:840	a membrane protein	823:840	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	6	35	theme	HAS2	772:775	arg1	protein					777:783	a recombinant human HAS2 protein	752:783	a recombinant human HAS2 protein with a histidine-tag	752:804	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	6	36	with	protein	777:783	arg1	histidine-tag					792:804	a histidine-tag	790:804	a histidine-tag	790:804	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	6	37	theme	affinity	920:927	arg1	chromatography					929:942	affinity chromatography	920:942	affinity chromatography	920:942	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	11	38	theme	present	1589:1595	arg1	data					1597:1600	the present data	1585:1600	the present data	1585:1600	Taken together, the present data suggests a clear functional association between HAS activity and cholesterol-dependent alterations in the physical and chemical properties of cell membranes.
24333423	0	39	theme	lipid	29:33	arg1	environment					35:45	a cholesterol-rich lipid environment	10:45	a cholesterol-rich lipid environment	10:45	Effect of a cholesterol-rich lipid environment on the enzymatic activity of reconstituted hyaluronan synthase.
24333423	0	40	theme	cholesterol-rich	12:27	arg1	environment					35:45	a cholesterol-rich lipid environment	10:45	a cholesterol-rich lipid environment	10:45	Effect of a cholesterol-rich lipid environment on the enzymatic activity of reconstituted hyaluronan synthase.
24333423	5	41	theme	hyaluronan	620:629	arg1	production					631:640	the hyaluronan production	616:640	the hyaluronan production of fibroblasts	616:655	The sequestration of cellular cholesterol by methyl-ß-cyclodextrin also decreased the hyaluronan production of fibroblasts, as well as the HAS activity.
24333423	8	42	theme	molar	1260:1264	arg1	ratio					1266:1270	the molar ratio	1256:1270	the molar ratio of phosphatidylcholine to cholesterol	1256:1308	Cholesterol regulates HAS2 activity in a biphasic manner, depending on the molar ratio of phosphatidylcholine to cholesterol.
24333423	8	43	theme	biphasic	1226:1233	arg1	manner					1235:1240	a biphasic manner	1224:1240	a biphasic manner	1224:1240	Cholesterol regulates HAS2 activity in a biphasic manner, depending on the molar ratio of phosphatidylcholine to cholesterol.
24333423	9	44	theme	cholesterol	1426:1436	arg1	presence					1403:1410	presence	1403:1410	presence	1403:1410	Furthermore, the activation profiles of different lipid compositions were determined in the presence or absence of cholesterol.
24333423	9	44	theme	cholesterol	1426:1436	arg1	absence					1415:1421	absence	1415:1421	absence	1415:1421	Furthermore, the activation profiles of different lipid compositions were determined in the presence or absence of cholesterol.
24333423	8	45	theme	HAS2	1207:1210	arg1	activity					1212:1219	HAS2 activity	1207:1219	HAS2 activity	1207:1219	Cholesterol regulates HAS2 activity in a biphasic manner, depending on the molar ratio of phosphatidylcholine to cholesterol.
24333423	9	46	theme	lipid	1361:1365	arg1	compositions					1367:1378	different lipid compositions	1351:1378	different lipid compositions	1351:1378	Furthermore, the activation profiles of different lipid compositions were determined in the presence or absence of cholesterol.
24333423	9	47	theme	compositions	1367:1378	arg1	profiles					1339:1346	the activation profiles	1324:1346	the activation profiles of different lipid compositions	1324:1378	Furthermore, the activation profiles of different lipid compositions were determined in the presence or absence of cholesterol.
24333423	11	48	theme	membranes	1749:1757	arg1	properties					1730:1739	the physical and chemical properties	1704:1739	the physical and chemical properties of cell membranes	1704:1757	Taken together, the present data suggests a clear functional association between HAS activity and cholesterol-dependent alterations in the physical and chemical properties of cell membranes.
24333423	10	49	from	activity	1487:1494	arg1	liposomes					1499:1507	liposomes	1499:1507	liposomes composed of phosphatidylethanolamine or phosphatidylserine	1499:1566	Cholesterol had the opposite effect on the HAS2 activity in liposomes composed of phosphatidylethanolamine or phosphatidylserine.
24333423	11	50	theme	functional	1619:1628	arg1	association					1630:1640	a clear functional association	1611:1640	a clear functional association between HAS activity and cholesterol-dependent alterations in the physical and chemical properties of cell membranes	1611:1757	Taken together, the present data suggests a clear functional association between HAS activity and cholesterol-dependent alterations in the physical and chemical properties of cell membranes.
24333423	4	51	theme	fibroblasts	451:461	arg1	culture					427:433	The culture	423:433	The culture of human dermal fibroblasts in lipoprotein-depleted medium	423:492	The culture of human dermal fibroblasts in lipoprotein-depleted medium attenuated the synthesis of hyaluronan.
24333423	5	52	theme	fibroblasts	645:655	arg1	production					631:640	the hyaluronan production	616:640	the hyaluronan production of fibroblasts	616:655	The sequestration of cellular cholesterol by methyl-ß-cyclodextrin also decreased the hyaluronan production of fibroblasts, as well as the HAS activity.
24333423	5	52	theme	fibroblasts	645:655	arg1	activity					677:684	the HAS activity	669:684	the HAS activity	669:684	The sequestration of cellular cholesterol by methyl-ß-cyclodextrin also decreased the hyaluronan production of fibroblasts, as well as the HAS activity.
24333423	6	53	theme	cholesterol	723:733	arg1	effects					712:718	the effects	708:718	the effects of cholesterol on HAS activity	708:749	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	7	54	theme	higher	1094:1099	arg1	activity					1108:1115	a higher enzyme activity	1092:1115	a higher enzyme activity	1092:1115	When the recombinant HAS2 proteins were reconstituted into liposomes composed of both saturated phosphatidylcholine and cholesterol, this provided a higher enzyme activity as compared with the liposomes formed by phosphatidylcholine alone.
24333423	3	55	from	association	343:353	arg1	membrane-environment					401:420	a cholesterol-rich membrane-environment	382:420	a cholesterol-rich membrane-environment	382:420	Here we investigated the functional association of hyaluronan synthesis in a cholesterol-rich membrane-environment.
24333423	6	56	theme	membrane	825:832	arg1	protein					834:840	a membrane protein	823:840	a membrane protein	823:840	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	6	56	theme	membrane	825:832	arg1	protein					777:783	a recombinant human HAS2 protein	752:783	a recombinant human HAS2 protein with a histidine-tag	752:804	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	10	57	theme	HAS2	1482:1485	arg1	activity					1487:1494	the HAS2 activity	1478:1494	the HAS2 activity in liposomes composed of phosphatidylethanolamine or phosphatidylserine	1478:1566	Cholesterol had the opposite effect on the HAS2 activity in liposomes composed of phosphatidylethanolamine or phosphatidylserine.
24333423	5	58	dep	the	669:671	arg1	HAS					673:675	HAS	673:675	HAS	673:675	The sequestration of cellular cholesterol by methyl-ß-cyclodextrin also decreased the hyaluronan production of fibroblasts, as well as the HAS activity.
24333423	6	59	theme	human	766:770	arg1	protein					834:840	a membrane protein	823:840	a membrane protein	823:840	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	6	59	theme	human	766:770	arg1	protein					777:783	a recombinant human HAS2 protein	752:783	a recombinant human HAS2 protein with a histidine-tag	752:804	To directly evaluate the effects of cholesterol on HAS activity, a recombinant human HAS2 protein with a histidine-tag was expressed as a membrane protein by using a baculovirus system, then successfully solubilized, and isolated by affinity chromatography.
24333423	4	60	theme	lipoprotein-depleted	466:485	arg1	medium					487:492	lipoprotein-depleted medium	466:492	lipoprotein-depleted medium	466:492	The culture of human dermal fibroblasts in lipoprotein-depleted medium attenuated the synthesis of hyaluronan.
24333423	11	61	theme	clear	1613:1617	arg1	association					1630:1640	a clear functional association	1611:1640	a clear functional association between HAS activity and cholesterol-dependent alterations in the physical and chemical properties of cell membranes	1611:1757	Taken together, the present data suggests a clear functional association between HAS activity and cholesterol-dependent alterations in the physical and chemical properties of cell membranes.
24333423	11	62	theme	cell	1744:1747	arg1	membranes					1749:1757	cell membranes	1744:1757	cell membranes	1744:1757	Taken together, the present data suggests a clear functional association between HAS activity and cholesterol-dependent alterations in the physical and chemical properties of cell membranes.
24333423	0	63	theme	enzymatic	54:62	arg1	activity					64:71	the enzymatic activity	50:71	the enzymatic activity of reconstituted hyaluronan synthase	50:108	Effect of a cholesterol-rich lipid environment on the enzymatic activity of reconstituted hyaluronan synthase.
24333423	10	64	theme	opposite	1459:1466	arg1	effect					1468:1473	the opposite effect	1455:1473	the opposite effect	1455:1473	Cholesterol had the opposite effect on the HAS2 activity in liposomes composed of phosphatidylethanolamine or phosphatidylserine.
28774805	0	0	theme	biological	115:124	arg1	properties					126:135	enhanced mechanical and biological properties	91:135	enhanced mechanical and biological properties	91:135	Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering.
28774805	1	1	theme	improved	379:386	arg1	properties					414:423	improved mechanical and biological properties	379:423	improved mechanical and biological properties	379:423	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	4	2	theme	pH	835:836	arg1	study					862:866	pH and blood compatibility study	835:866	pH and blood compatibility study	835:866	pH and blood compatibility study revealed that ZnCMH I-III should be nontoxic to the human body.
28774805	6	3	dep	strength	1145:1152	arg1	the					1125:1127	the	1125:1127	the	1125:1127	Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity.
28774805	6	4	theme	best	1129:1132	arg1	strength					1145:1152	mechanical strength	1134:1152	mechanical strength	1134:1152	Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity.
28774805	1	5	theme	mechanical	388:397	arg1	properties					414:423	improved mechanical and biological properties	379:423	improved mechanical and biological properties	379:423	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	7	6	theme	tensile	1270:1276	arg1	strengths					1278:1286	the tensile strengths	1266:1286	the tensile strengths of ZnCMH I-III	1266:1301	Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite.
28774805	8	7	theme	OMMT	1373:1376	arg1	absence					1362:1368	absence	1362:1368	absence of OMMT	1362:1376	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	8	8	theme	ZnCMH	1598:1602	arg1	nanocomposite					1606:1618	ZnCMH I nanocomposite	1598:1618	ZnCMH I nanocomposite	1598:1618	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	8	9	theme	control	1379:1385	arg1	ZnCH					1395:1398	ZnCH	1395:1398	ZnCH	1395:1398	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	8	9	theme	control	1379:1385	arg1	sample					1387:1392	control sample	1379:1392	control sample (ZnCH)	1379:1399	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	2	10	theme	nanocomposites	690:703	arg1	composition					639:649	composition	639:649	composition	639:649	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	2	10	theme	nanocomposites	690:703	arg1	morphology					663:672	surface morphology	655:672	surface morphology	655:672	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	1	11	theme	chitosan/hydroxyapatite-zinc	296:323	arg1	CTS/HAP-ZnO					332:342	CTS/HAP-ZnO	332:342	CTS/HAP-ZnO	332:342	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	1	11	theme	chitosan/hydroxyapatite-zinc	296:323	arg1	oxide					325:329	chitosan/hydroxyapatite-zinc oxide	296:329	chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties	296:423	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	1	12	theme	biological	403:412	arg1	properties					414:423	improved mechanical and biological properties	379:423	improved mechanical and biological properties	379:423	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	2	13	theme	prepared	681:688	arg1	nanocomposites					690:703	the prepared nanocomposites	677:703	the prepared nanocomposites	677:703	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	7	14	theme	ZnCMH	1291:1295	arg1	strengths					1278:1286	the tensile strengths	1266:1286	the tensile strengths of ZnCMH I-III	1266:1301	Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite.
28774805	8	15	theme	osteoblastic	1487:1498	arg1	cell					1500:1503	osteoblastic cell	1487:1503	osteoblastic cell	1487:1503	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	1	16	theme	oxide	325:329	arg1	ZnCMH					361:365	ZnCMH	361:365	ZnCMH	361:365	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	1	16	theme	oxide	325:329	arg1	nanocomposites					345:358	chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites	296:358	chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties	296:423	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	7	17	theme	CTS-OMMT-HAP	1335:1346	arg1	composite					1348:1356	previously reported CTS-OMMT-HAP composite	1315:1356	previously reported CTS-OMMT-HAP composite	1315:1356	Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite.
28774805	5	18	theme	human	979:983	arg1	cells					1004:1008	human osteoblastic MG-63 cells	979:1008	human osteoblastic MG-63 cells	979:1008	Cytocompatibility of these nanocomposites with human osteoblastic MG-63 cells was also established.
28774805	6	19	theme	water	1158:1162	arg1	capacity					1175:1182	water absorption capacity	1158:1182	water absorption capacity	1158:1182	Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity.
28774805	2	20	dep	composition	639:649	arg1	the					635:637	the	635:637	the	635:637	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	6	21	theme	Experimental	1032:1043	arg1	findings					1045:1052	Experimental findings	1032:1052	Experimental findings	1032:1052	Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity.
28774805	2	22	theme	electron	557:564	arg1	microscopy					566:575	scanning electron microscopy	548:575	scanning electron microscopy	548:575	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	8	23	theme	bone	1659:1662	arg1	engineering					1671:1681	bone tissue engineering	1659:1681	bone tissue engineering	1659:1681	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	7	24	theme	reported	1326:1333	arg1	composite					1348:1356	previously reported CTS-OMMT-HAP composite	1315:1356	previously reported CTS-OMMT-HAP composite	1315:1356	Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite.
28774805	1	25	theme	study	207:211	arg1	objective					189:197	The objective	185:197	The objective of this study	185:211	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	0	26	theme	modified	12:19	arg1	clay					21:24	Organically modified clay	0:24	Organically modified clay	0:24	Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering.
28774805	8	27	theme	tissue	1664:1669	arg1	engineering					1671:1681	bone tissue engineering	1659:1681	bone tissue engineering	1659:1681	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	7	28	theme	ZnO	1207:1209	arg1	nanoparticles					1211:1223	ZnO nanoparticles	1207:1223	ZnO nanoparticles	1207:1223	Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite.
28774805	1	29	with	nanocomposites	345:358	arg1	properties					414:423	improved mechanical and biological properties	379:423	improved mechanical and biological properties	379:423	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	2	30	theme	powder	522:527	arg1	diffraction					535:545	powder X-ray diffraction	522:545	powder X-ray diffraction	522:545	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	7	31	theme	nanoparticles	1211:1223	arg1	%					1202:1202	0.1wt%	1197:1202	0.1wt% of ZnO nanoparticles	1197:1223	Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite.
28774805	7	31	theme	nanoparticles	1211:1223	arg1	nanoparticles					1211:1223	ZnO nanoparticles	1207:1223	ZnO nanoparticles	1207:1223	Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite.
28774805	3	32	theme	bacterial	783:791	arg1	strains					793:799	both Gram-positive and Gram-negative bacterial strains	746:799	both Gram-positive and Gram-negative bacterial strains	746:799	Strong antibacterial properties against both Gram-positive and Gram-negative bacterial strains were established for ZnCMH I-III.
28774805	6	33	theme	ZnCMH	1110:1114	arg1	CTS/HAP-ZnO					1097:1107	CTS/HAP-ZnO	1097:1107	CTS/HAP-ZnO (ZnCMH I)	1097:1117	Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity.
28774805	6	33	theme	ZnCMH	1110:1114	arg1	I					1116:1116	ZnCMH I	1110:1116	ZnCMH I	1110:1116	Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity.
28774805	0	34	theme	bone	160:163	arg1	engineering					172:182	bone tissue engineering	160:182	bone tissue engineering	160:182	Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering.
28774805	8	35	theme	above-mentioned	1549:1563	arg1	studies					1565:1571	the above-mentioned studies	1545:1571	the above-mentioned studies	1545:1571	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	5	36	with	Cytocompatibility	932:948	arg1	cells					1004:1008	human osteoblastic MG-63 cells	979:1008	human osteoblastic MG-63 cells	979:1008	Cytocompatibility of these nanocomposites with human osteoblastic MG-63 cells was also established.
28774805	5	37	theme	nanocomposites	959:972	arg1	Cytocompatibility					932:948	Cytocompatibility	932:948	Cytocompatibility of these nanocomposites with human osteoblastic MG-63 cells	932:1008	Cytocompatibility of these nanocomposites with human osteoblastic MG-63 cells was also established.
28774805	3	38	theme	Gram-negative	769:781	arg1	strains					793:799	both Gram-positive and Gram-negative bacterial strains	746:799	both Gram-positive and Gram-negative bacterial strains	746:799	Strong antibacterial properties against both Gram-positive and Gram-negative bacterial strains were established for ZnCMH I-III.
28774805	2	39	theme	scanning	548:555	arg1	microscopy					566:575	scanning electron microscopy	548:575	scanning electron microscopy	548:575	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	3	40	theme	Gram-positive	751:763	arg1	strains					793:799	both Gram-positive and Gram-negative bacterial strains	746:799	both Gram-positive and Gram-negative bacterial strains	746:799	Strong antibacterial properties against both Gram-positive and Gram-negative bacterial strains were established for ZnCMH I-III.
28774805	1	41	theme	biomimetic	226:235	arg1	OMMT					280:283	OMMT	280:283	OMMT	280:283	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	1	41	theme	biomimetic	226:235	arg1	clay					274:277	biomimetic organically modified montmorillonite clay	226:277	biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite	226:478	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	0	42	theme	oxide	65:69	arg1	nanocomposites					71:84	chitosan/hydroxyapatite-zinc oxide nanocomposites	36:84	chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering	36:182	Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering.
28774805	8	43	theme	tensile	1421:1427	arg1	strength					1429:1436	reduced tensile strength	1413:1436	reduced tensile strength	1413:1436	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	1	44	theme	reported	448:455	arg1	composite					470:478	previously reported CTS/OMMT/HAP composite	437:478	previously reported CTS/OMMT/HAP composite	437:478	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	2	45	dep	Fourier	481:487	arg1	transform					489:497	transform	489:497	transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy	489:612	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	5	46	theme	osteoblastic	985:996	arg1	cells					1004:1008	human osteoblastic MG-63 cells	979:1008	human osteoblastic MG-63 cells	979:1008	Cytocompatibility of these nanocomposites with human osteoblastic MG-63 cells was also established.
28774805	0	47	theme	chitosan/hydroxyapatite-zinc	36:63	arg1	nanocomposites					71:84	chitosan/hydroxyapatite-zinc oxide nanocomposites	36:84	chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering	36:182	Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering.
28774805	0	48	theme	tissue	165:170	arg1	engineering					172:182	bone tissue engineering	160:182	bone tissue engineering	160:182	Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering.
28774805	1	49	theme	CTS/OMMT/HAP	457:468	arg1	composite					470:478	previously reported CTS/OMMT/HAP composite	437:478	previously reported CTS/OMMT/HAP composite	437:478	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	3	50	theme	Strong	706:711	arg1	properties					727:736	Strong antibacterial properties	706:736	Strong antibacterial properties against both Gram-positive and Gram-negative bacterial strains	706:799	Strong antibacterial properties against both Gram-positive and Gram-negative bacterial strains were established for ZnCMH I-III.
28774805	1	51	theme	modified	249:256	arg1	OMMT					280:283	OMMT	280:283	OMMT	280:283	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	1	51	theme	modified	249:256	arg1	clay					274:277	biomimetic organically modified montmorillonite clay	226:277	biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite	226:478	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	2	52	theme	electron	594:601	arg1	microscopy					603:612	transmission electron microscopy	581:612	transmission electron microscopy	581:612	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	6	53	theme	absorption	1164:1173	arg1	capacity					1175:1182	water absorption capacity	1158:1182	water absorption capacity	1158:1182	Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity.
28774805	2	54	theme	transmission	581:592	arg1	microscopy					603:612	transmission electron microscopy	581:612	transmission electron microscopy	581:612	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	4	55	theme	blood	842:846	arg1	compatibility					848:860	blood compatibility	842:860	blood compatibility	842:860	pH and blood compatibility study revealed that ZnCMH I-III should be nontoxic to the human body.
28774805	7	56	theme	0.1wt	1197:1201	arg1	%					1202:1202	0.1wt%	1197:1202	0.1wt% of ZnO nanoparticles	1197:1223	Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite.
28774805	7	56	theme	0.1wt	1197:1201	arg1	nanoparticles					1211:1223	ZnO nanoparticles	1207:1223	ZnO nanoparticles	1207:1223	Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite.
28774805	8	57	with	effect	1453:1458	arg1	cell					1500:1503	osteoblastic cell	1487:1503	osteoblastic cell	1487:1503	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	7	58	theme	%	1202:1202	arg1	Addition					1185:1192	Addition	1185:1192	Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP	1185:1241	Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite.
28774805	0	59	from	application	145:155	arg1	engineering					172:182	bone tissue engineering	160:182	bone tissue engineering	160:182	Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering.
28774805	2	60	dep	transform	489:497	arg1	infrared					499:506	infrared	499:506	transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy	489:612	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	2	61	used	used	619:622	arg2	Fourier					481:487	Fourier	481:487	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy	481:612	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	6	62	theme	%	1082:1082	arg1	addition					1067:1074	addition	1067:1074	addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I)	1067:1117	Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity.
28774805	8	63	theme	reduced	1413:1419	arg1	strength					1429:1436	reduced tensile strength	1413:1436	reduced tensile strength	1413:1436	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	8	64	with	cytocompatibility	1464:1480	arg1	cell					1500:1503	osteoblastic cell	1487:1503	osteoblastic cell	1487:1503	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	4	65	theme	human	920:924	arg1	body					926:929	the human body	916:929	the human body	916:929	pH and blood compatibility study revealed that ZnCMH I-III should be nontoxic to the human body.
28774805	8	66	theme	antibacterial	1439:1451	arg1	effect					1453:1458	antibacterial effect	1439:1458	antibacterial effect	1439:1458	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	1	67	theme	montmorillonite	258:272	arg1	OMMT					280:283	OMMT	280:283	OMMT	280:283	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	1	67	theme	montmorillonite	258:272	arg1	clay					274:277	biomimetic organically modified montmorillonite clay	226:277	biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite	226:478	The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite.
28774805	2	68	theme	surface	655:661	arg1	morphology					663:672	surface morphology	655:672	surface morphology	655:672	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	8	69	theme	great	1626:1630	arg1	potential					1632:1640	a great potential	1624:1640	a great potential to be applied in bone tissue engineering	1624:1681	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	4	70	theme	compatibility	848:860	arg1	study					862:866	pH and blood compatibility study	835:866	pH and blood compatibility study	835:866	pH and blood compatibility study revealed that ZnCMH I-III should be nontoxic to the human body.
28774805	0	71	theme	mechanical	100:109	arg1	properties					126:135	enhanced mechanical and biological properties	91:135	enhanced mechanical and biological properties	91:135	Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering.
28774805	5	72	theme	MG-63	998:1002	arg1	cells					1004:1008	human osteoblastic MG-63 cells	979:1008	human osteoblastic MG-63 cells	979:1008	Cytocompatibility of these nanocomposites with human osteoblastic MG-63 cells was also established.
28774805	3	73	theme	antibacterial	713:725	arg1	properties					727:736	Strong antibacterial properties	706:736	Strong antibacterial properties against both Gram-positive and Gram-negative bacterial strains	706:799	Strong antibacterial properties against both Gram-positive and Gram-negative bacterial strains were established for ZnCMH I-III.
28774805	0	74	with	nanocomposites	71:84	arg1	properties					126:135	enhanced mechanical and biological properties	91:135	enhanced mechanical and biological properties	91:135	Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering.
28774805	8	75	contain	has	1620:1622	arg2	potential					1632:1640	a great potential	1624:1640	a great potential to be applied in bone tissue engineering	1624:1681	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	8	75	contain	has	1620:1622	arg1	nanocomposite					1606:1618	ZnCMH I nanocomposite	1598:1618	ZnCMH I nanocomposite	1598:1618	In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.
28774805	0	76	theme	enhanced	91:98	arg1	properties					126:135	enhanced mechanical and biological properties	91:135	enhanced mechanical and biological properties	91:135	Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering.
28774805	6	77	theme	mechanical	1134:1143	arg1	strength					1145:1152	mechanical strength	1134:1152	mechanical strength	1134:1152	Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity.
28774805	2	78	theme	X-ray	529:533	arg1	diffraction					535:545	powder X-ray diffraction	522:545	powder X-ray diffraction	522:545	Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites.
28774805	6	79	theme	OMMT	1087:1090	arg1	OMMT					1087:1090	OMMT	1087:1090	OMMT	1087:1090	Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity.
28774805	6	79	theme	OMMT	1087:1090	arg1	%					1082:1082	5wt%	1079:1082	5wt% of OMMT	1079:1090	Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity.
27773278	3	0	theme	predicted	822:830	arg1	value					832:836	predicted value	822:836	predicted value	822:836	The optimal condition was validated through experiments and the observed value was interrelated with predicted value.
27773278	1	1	theme	variables	247:255	arg1	effect					229:234	the effect	225:234	the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel	225:467	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	2	dep	factors	94:100	arg1	design					163:168	three level face centered central composite response surface design	102:168	Four factors three level face centered central composite response surface design	89:168	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	3	theme	liquid-solid	258:269	arg1	ratio					276:280	liquid-solid (LS) ratio	258:280	liquid-solid (LS) ratio (10:1-20:1ml/g)	258:296	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	3	theme	liquid-solid	258:269	arg1	10:1-20:1ml/g					283:295	10:1-20:1ml/g	283:295	10:1-20:1ml/g	283:295	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	4	theme	sonication	309:318	arg1	time					320:323	sonication time	309:323	sonication time (15-30min)	309:334	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	4	theme	sonication	309:318	arg1	15-30min					326:333	15-30min	326:333	15-30min	326:333	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	2	5	theme	15:1ml/g	650:657	arg1	ratio					641:645	Liquid-solid ratio	628:645	Liquid-solid ratio of 15:1ml/g	628:657	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	1	6	theme	maximum	381:387	arg1	yield					400:404	the maximum extraction yield	377:404	the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel	377:467	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	7	theme	ultrasound	472:481	arg1	method					503:508	ultrasound assisted extraction method	472:508	ultrasound assisted extraction method	472:508	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	0	8	from	waste	46:50	arg1	extraction					20:29	Ultrasound assisted extraction	0:29	Ultrasound assisted extraction of pectin from waste	0:50	Ultrasound assisted extraction of pectin from waste Artocarpus heterophyllus fruit peel.
27773278	2	9	theme	following	575:583	arg1	follows					619:625	follows	619:625	follows	619:625	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	2	9	theme	following	575:583	arg1	condition					593:601	the following optimal condition	571:601	the following optimal condition	571:601	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	1	10	theme	extraction	389:398	arg1	yield					400:404	the maximum extraction yield	377:404	the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel	377:467	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	11	theme	assisted	483:490	arg1	method					503:508	ultrasound assisted extraction method	472:508	ultrasound assisted extraction method	472:508	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	12	from	peel	464:467	arg1	yield					400:404	the maximum extraction yield	377:404	the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel	377:467	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	2	13	theme	Liquid-solid	628:639	arg1	ratio					641:645	Liquid-solid ratio	628:645	Liquid-solid ratio of 15:1ml/g	628:657	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	1	14	theme	extraction	492:501	arg1	method					503:508	ultrasound assisted extraction method	472:508	ultrasound assisted extraction method	472:508	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	0	15	theme	assisted	11:18	arg1	extraction					20:29	Ultrasound assisted extraction	0:29	Ultrasound assisted extraction of pectin from waste	0:50	Ultrasound assisted extraction of pectin from waste Artocarpus heterophyllus fruit peel.
27773278	2	16	theme	sonication	671:680	arg1	follows					619:625	follows	619:625	follows	619:625	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	2	16	theme	sonication	671:680	arg1	time					682:685	sonication time	671:685	sonication time of 24min and temperature of 60°C	671:718	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	0	17	theme	Ultrasound	0:9	arg1	extraction					20:29	Ultrasound assisted extraction	0:29	Ultrasound assisted extraction of pectin from waste	0:50	Ultrasound assisted extraction of pectin from waste Artocarpus heterophyllus fruit peel.
27773278	1	18	theme	LS	272:273	arg1	ratio					276:280	liquid-solid (LS) ratio	258:280	liquid-solid (LS) ratio (10:1-20:1ml/g)	258:296	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	18	theme	LS	272:273	arg1	10:1-20:1ml/g					283:295	10:1-20:1ml/g	283:295	10:1-20:1ml/g	283:295	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	19	theme	pectin	409:414	arg1	yield					400:404	the maximum extraction yield	377:404	the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel	377:467	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	0	20	dep	peel	83:86	arg1	extraction					20:29	Ultrasound assisted extraction	0:29	Ultrasound assisted extraction of pectin from waste	0:50	Ultrasound assisted extraction of pectin from waste Artocarpus heterophyllus fruit peel.
27773278	2	21	theme	60°C	715:718	arg1	temperature					700:710	temperature	700:710	temperature	700:710	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	2	21	theme	60°C	715:718	arg1	24min					690:694	24min	690:694	24min	690:694	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	2	22	theme	optimal	585:591	arg1	follows					619:625	follows	619:625	follows	619:625	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	2	22	theme	optimal	585:591	arg1	condition					593:601	the following optimal condition	571:601	the following optimal condition	571:601	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	1	23	theme	level	108:112	arg1	design					163:168	three level face centered central composite response surface design	102:168	Four factors three level face centered central composite response surface design	89:168	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	2	24	theme	temperature	700:710	arg1	follows					619:625	follows	619:625	follows	619:625	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	2	24	theme	temperature	700:710	arg1	time					682:685	sonication time	671:685	sonication time of 24min and temperature of 60°C	671:718	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	2	25	theme	24min	690:694	arg1	follows					619:625	follows	619:625	follows	619:625	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	2	25	theme	24min	690:694	arg1	time					682:685	sonication time	671:685	sonication time of 24min and temperature of 60°C	671:718	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	0	26	theme	pectin	34:39	arg1	extraction					20:29	Ultrasound assisted extraction	0:29	Ultrasound assisted extraction of pectin from waste	0:50	Ultrasound assisted extraction of pectin from waste Artocarpus heterophyllus fruit peel.
27773278	1	27	theme	extraction	340:349	arg1	temperature					351:361	extraction temperature	340:361	extraction temperature	340:361	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	28	theme	waste	421:425	arg1	peel					464:467	waste Artocarpus heterophyllus (Jackfruit) peel	421:467	waste Artocarpus heterophyllus (Jackfruit) peel	421:467	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	0	29	theme	Artocarpus	52:61	arg1	peel					83:86	Artocarpus heterophyllus fruit peel	52:86	Artocarpus heterophyllus fruit peel	52:86	Ultrasound assisted extraction of pectin from waste Artocarpus heterophyllus fruit peel.
27773278	1	30	theme	Artocarpus	427:436	arg1	peel					464:467	waste Artocarpus heterophyllus (Jackfruit) peel	421:467	waste Artocarpus heterophyllus (Jackfruit) peel	421:467	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	31	theme	face	114:117	arg1	design					163:168	three level face centered central composite response surface design	102:168	Four factors three level face centered central composite response surface design	89:168	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	2	32	theme	optimization	521:532	arg1	method					534:539	Numerical optimization method	511:539	Numerical optimization method	511:539	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	1	33	theme	heterophyllus	438:450	arg1	peel					464:467	waste Artocarpus heterophyllus (Jackfruit) peel	421:467	waste Artocarpus heterophyllus (Jackfruit) peel	421:467	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	34	theme	centered	119:126	arg1	design					163:168	three level face centered central composite response surface design	102:168	Four factors three level face centered central composite response surface design	89:168	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	2	35	dep	follows	619:625	arg1	ratio					641:645	Liquid-solid ratio	628:645	Liquid-solid ratio of 15:1ml/g	628:657	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	2	36	theme	Numerical	511:519	arg1	method					534:539	Numerical optimization method	511:539	Numerical optimization method	511:539	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	3	37	theme	observed	785:792	arg1	value					794:798	the observed value	781:798	the observed value	781:798	The optimal condition was validated through experiments and the observed value was interrelated with predicted value.
27773278	1	38	theme	central	128:134	arg1	design					163:168	three level face centered central composite response surface design	102:168	Four factors three level face centered central composite response surface design	89:168	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	3	39	theme	optimal	725:731	arg1	condition					733:741	The optimal condition	721:741	The optimal condition	721:741	The optimal condition was validated through experiments and the observed value was interrelated with predicted value.
27773278	1	40	from	effect	229:234	arg1	yield					400:404	the maximum extraction yield	377:404	the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel	377:467	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	41	dep	variables	247:255	arg1	ratio					276:280	liquid-solid (LS) ratio	258:280	liquid-solid (LS) ratio (10:1-20:1ml/g)	258:296	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	41	dep	variables	247:255	arg1	10:1-20:1ml/g					283:295	10:1-20:1ml/g	283:295	10:1-20:1ml/g	283:295	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	42	theme	Jackfruit	453:461	arg1	peel					464:467	waste Artocarpus heterophyllus (Jackfruit) peel	421:467	waste Artocarpus heterophyllus (Jackfruit) peel	421:467	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	43	theme	composite	136:144	arg1	design					163:168	three level face centered central composite response surface design	102:168	Four factors three level face centered central composite response surface design	89:168	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	0	44	theme	fruit	77:81	arg1	peel					83:86	Artocarpus heterophyllus fruit peel	52:86	Artocarpus heterophyllus fruit peel	52:86	Ultrasound assisted extraction of pectin from waste Artocarpus heterophyllus fruit peel.
27773278	2	45	theme	1.6	666:668	arg1	follows					619:625	follows	619:625	follows	619:625	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	2	45	theme	1.6	666:668	arg1	pH					660:661	pH	660:661	pH of 1.6	660:668	Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C.
27773278	1	46	theme	response	146:153	arg1	design					163:168	three level face centered central composite response surface design	102:168	Four factors three level face centered central composite response surface design	89:168	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	0	47	theme	heterophyllus	63:75	arg1	peel					83:86	Artocarpus heterophyllus fruit peel	52:86	Artocarpus heterophyllus fruit peel	52:86	Ultrasound assisted extraction of pectin from waste Artocarpus heterophyllus fruit peel.
27773278	1	48	dep	ratio	276:280	arg1	15-30min					326:333	15-30min	326:333	15-30min	326:333	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	48	dep	ratio	276:280	arg1	time					320:323	sonication time	309:323	sonication time (15-30min)	309:334	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	48	dep	ratio	276:280	arg1	temperature					351:361	extraction temperature	340:361	extraction temperature	340:361	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	48	dep	ratio	276:280	arg1	50-70°C					364:370	50-70°C	364:370	50-70°C	364:370	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	48	dep	ratio	276:280	arg1	pH					299:300	pH (1-2)	299:306	pH (1-2)	299:306	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	49	dep	investigate	200:210	arg1	optimize					216:223	optimize	216:223	optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method	216:508	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	50	theme	surface	155:161	arg1	design					163:168	three level face centered central composite response surface design	102:168	Four factors three level face centered central composite response surface design	89:168	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
27773278	1	51	theme	process	239:245	arg1	variables					247:255	process variables	239:255	process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C))	239:372	Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method.
26235712	5	0	theme	cell	795:798	arg1	growth					800:805	cell growth	795:805	cell growth	795:805	Cell adhesion rate, cell proliferation, and cell activity were evaluated, and cell growth and formation of mineralized nodules were observed.
26235712	2	1	dep	3-	422:423	arg1	to					419:420	to	419:420	to	419:420	Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3- to fabricate the composite materials.
26235712	3	2	theme	optimum	479:485	arg1	ratio					494:498	the optimum mixing ratio	475:498	the optimum mixing ratio of SF and CS	475:511	To find out the optimum mixing ratio of SF and CS, parameters such as pore size, porosity, water absorption, and the mechanical properties were evaluated.
26235712	4	3	theme	SF-CS	659:663	arg1	scaffolds					665:673	SF-CS scaffolds	659:673	SF-CS scaffolds	659:673	Osteoblast cells hFOB1.19 were seeded on SF-CS scaffolds and pure CS scaffolds for the first time.
26235712	5	4	theme	mineralized	824:834	arg1	nodules					836:842	mineralized nodules	824:842	mineralized nodules	824:842	Cell adhesion rate, cell proliferation, and cell activity were evaluated, and cell growth and formation of mineralized nodules were observed.
26235712	3	5	theme	mixing	487:492	arg1	ratio					494:498	the optimum mixing ratio	475:498	the optimum mixing ratio of SF and CS	475:511	To find out the optimum mixing ratio of SF and CS, parameters such as pore size, porosity, water absorption, and the mechanical properties were evaluated.
26235712	6	6	theme	SF-CS	879:883	arg1	candidate					910:918	a suitable candidate	899:918	a suitable candidate for bone tissue engineering	899:946	Results showed that SF-CS scaffolds are a suitable candidate for bone tissue engineering.
26235712	6	6	theme	SF-CS	879:883	arg1	scaffolds					885:893	SF-CS scaffolds	879:893	SF-CS scaffolds	879:893	Results showed that SF-CS scaffolds are a suitable candidate for bone tissue engineering.
26235712	5	7	theme	cell	737:740	arg1	proliferation					742:754	cell proliferation	737:754	cell proliferation	737:754	Cell adhesion rate, cell proliferation, and cell activity were evaluated, and cell growth and formation of mineralized nodules were observed.
26235712	4	8	theme	CS	684:685	arg1	scaffolds					687:695	pure CS scaffolds	679:695	pure CS scaffolds	679:695	Osteoblast cells hFOB1.19 were seeded on SF-CS scaffolds and pure CS scaffolds for the first time.
26235712	2	9	theme	different	379:387	arg1	ratios					389:394	different ratios -3 to 7, 5 to 5, and 7 to 3-	379:423	different ratios -3 to 7, 5 to 5, and 7 to 3-	379:423	Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3- to fabricate the composite materials.
26235712	2	10	theme	composite	442:450	arg1	materials					452:460	the composite materials	438:460	the composite materials	438:460	Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3- to fabricate the composite materials.
26235712	3	11	theme	SF	503:504	arg1	ratio					494:498	the optimum mixing ratio	475:498	the optimum mixing ratio of SF and CS	475:511	To find out the optimum mixing ratio of SF and CS, parameters such as pore size, porosity, water absorption, and the mechanical properties were evaluated.
26235712	5	12	theme	Cell	717:720	arg1	rate					731:734	Cell adhesion rate	717:734	Cell adhesion rate	717:734	Cell adhesion rate, cell proliferation, and cell activity were evaluated, and cell growth and formation of mineralized nodules were observed.
26235712	1	13	theme	bone	294:297	arg1	scaffolds					318:326	bone tissue engineering scaffolds	294:326	bone tissue engineering scaffolds	294:326	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	2	14	dep	5	410:410	arg1	to					407:408	to	407:408	to	407:408	Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3- to fabricate the composite materials.
26235712	5	15	theme	nodules	836:842	arg1	formation					811:819	formation	811:819	formation	811:819	Cell adhesion rate, cell proliferation, and cell activity were evaluated, and cell growth and formation of mineralized nodules were observed.
26235712	5	15	theme	nodules	836:842	arg1	growth					800:805	cell growth	795:805	cell growth	795:805	Cell adhesion rate, cell proliferation, and cell activity were evaluated, and cell growth and formation of mineralized nodules were observed.
26235712	4	16	theme	pure	679:682	arg1	scaffolds					687:695	pure CS scaffolds	679:695	pure CS scaffolds	679:695	Osteoblast cells hFOB1.19 were seeded on SF-CS scaffolds and pure CS scaffolds for the first time.
26235712	5	17	theme	adhesion	722:729	arg1	rate					731:734	Cell adhesion rate	717:734	Cell adhesion rate	717:734	Cell adhesion rate, cell proliferation, and cell activity were evaluated, and cell growth and formation of mineralized nodules were observed.
26235712	1	18	theme	tissue	299:304	arg1	scaffolds					318:326	bone tissue engineering scaffolds	294:326	bone tissue engineering scaffolds	294:326	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	2	19	theme	Silk	329:332	arg1	SF					343:344	SF	343:344	SF	343:344	Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3- to fabricate the composite materials.
26235712	2	19	theme	Silk	329:332	arg1	fibroin					334:340	Silk fibroin	329:340	Silk fibroin (SF)	329:345	Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3- to fabricate the composite materials.
26235712	1	20	theme	three-dimensional	206:222	arg1	scaffolds					254:262	three-dimensional silk fibroin-chitosan (SF-CS) scaffolds	206:262	three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds	206:326	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	0	21	theme	in	17:18	arg1	characterization					26:41	in vitro characterization	17:41	in vitro characterization of three-dimensional silk fibroinchitosan scaffolds	17:93	Construction and in vitro characterization of three-dimensional silk fibroinchitosan scaffolds.
26235712	1	22	theme	engineering	306:316	arg1	scaffolds					318:326	bone tissue engineering scaffolds	294:326	bone tissue engineering scaffolds	294:326	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	3	23	theme	water	554:558	arg1	absorption					560:569	water absorption	554:569	water absorption	554:569	To find out the optimum mixing ratio of SF and CS, parameters such as pore size, porosity, water absorption, and the mechanical properties were evaluated.
26235712	1	24	theme	study	118:122	arg1	objective					100:108	The objective	96:108	The objective of this study	96:122	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	1	25	theme	silk	224:227	arg1	scaffolds					254:262	three-dimensional silk fibroin-chitosan (SF-CS) scaffolds	206:262	three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds	206:326	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	1	26	theme	scaffolds	318:326	arg1	requirements					278:289	the requirements	274:289	the requirements of bone tissue engineering scaffolds	274:326	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	1	27	theme	fibroin-chitosan	229:244	arg1	scaffolds					254:262	three-dimensional silk fibroin-chitosan (SF-CS) scaffolds	206:262	three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds	206:326	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	6	28	theme	tissue	929:934	arg1	engineering					936:946	bone tissue engineering	924:946	bone tissue engineering	924:946	Results showed that SF-CS scaffolds are a suitable candidate for bone tissue engineering.
26235712	0	29	theme	silk	64:67	arg1	scaffolds					85:93	three-dimensional silk fibroinchitosan scaffolds	46:93	three-dimensional silk fibroinchitosan scaffolds	46:93	Construction and in vitro characterization of three-dimensional silk fibroinchitosan scaffolds.
26235712	3	30	theme	CS	510:511	arg1	ratio					494:498	the optimum mixing ratio	475:498	the optimum mixing ratio of SF and CS	475:511	To find out the optimum mixing ratio of SF and CS, parameters such as pore size, porosity, water absorption, and the mechanical properties were evaluated.
26235712	0	31	theme	three-dimensional	46:62	arg1	scaffolds					85:93	three-dimensional silk fibroinchitosan scaffolds	46:93	three-dimensional silk fibroinchitosan scaffolds	46:93	Construction and in vitro characterization of three-dimensional silk fibroinchitosan scaffolds.
26235712	2	32	dep	7	402:402	arg1	to					399:400	to	399:400	to	399:400	Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3- to fabricate the composite materials.
26235712	4	33	theme	Osteoblast	618:627	arg1	cells					629:633	Osteoblast cells hFOB1.19	618:642	Osteoblast cells hFOB1.19	618:642	Osteoblast cells hFOB1.19 were seeded on SF-CS scaffolds and pure CS scaffolds for the first time.
26235712	4	34	theme	first	705:709	arg1	time					711:714	the first time	701:714	the first time	701:714	Osteoblast cells hFOB1.19 were seeded on SF-CS scaffolds and pure CS scaffolds for the first time.
26235712	3	35	theme	mechanical	580:589	arg1	properties					591:600	the mechanical properties	576:600	the mechanical properties	576:600	To find out the optimum mixing ratio of SF and CS, parameters such as pore size, porosity, water absorption, and the mechanical properties were evaluated.
26235712	1	36	theme	SF-CS	247:251	arg1	scaffolds					254:262	three-dimensional silk fibroin-chitosan (SF-CS) scaffolds	206:262	three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds	206:326	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	0	37	dep	in	17:18	arg1	vitro					20:24	vitro	20:24	vitro	20:24	Construction and in vitro characterization of three-dimensional silk fibroinchitosan scaffolds.
26235712	0	38	theme	scaffolds	85:93	arg1	Construction					0:11	Construction	0:11	Construction	0:11	Construction and in vitro characterization of three-dimensional silk fibroinchitosan scaffolds.
26235712	0	38	theme	scaffolds	85:93	arg1	characterization					26:41	in vitro characterization	17:41	in vitro characterization of three-dimensional silk fibroinchitosan scaffolds	17:93	Construction and in vitro characterization of three-dimensional silk fibroinchitosan scaffolds.
26235712	2	39	dep	ratios	389:394	arg1	5					410:410	5	410:410	5	410:410	Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3- to fabricate the composite materials.
26235712	2	39	dep	ratios	389:394	arg1	7					402:402	7	402:402	7	402:402	Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3- to fabricate the composite materials.
26235712	2	39	dep	ratios	389:394	arg1	3-					422:423	3-	422:423	3-	422:423	Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3- to fabricate the composite materials.
26235712	1	40	theme	construction	143:154	arg1	method					156:161	the construction method	139:161	the construction method	139:161	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	5	41	theme	cell	761:764	arg1	activity					766:773	cell activity	761:773	cell activity	761:773	Cell adhesion rate, cell proliferation, and cell activity were evaluated, and cell growth and formation of mineralized nodules were observed.
26235712	1	42	theme	scaffolds	254:262	arg1	biocompatibility					186:201	biocompatibility	186:201	biocompatibility	186:201	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	1	42	theme	scaffolds	254:262	arg1	method					156:161	the construction method	139:161	the construction method	139:161	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	1	42	theme	scaffolds	254:262	arg1	characterization					164:179	characterization	164:179	characterization	164:179	The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds.
26235712	0	43	theme	fibroinchitosan	69:83	arg1	scaffolds					85:93	three-dimensional silk fibroinchitosan scaffolds	46:93	three-dimensional silk fibroinchitosan scaffolds	46:93	Construction and in vitro characterization of three-dimensional silk fibroinchitosan scaffolds.
26235712	3	44	theme	pore	533:536	arg1	size					538:541	pore size	533:541	pore size	533:541	To find out the optimum mixing ratio of SF and CS, parameters such as pore size, porosity, water absorption, and the mechanical properties were evaluated.
26235712	6	45	theme	bone	924:927	arg1	engineering					936:946	bone tissue engineering	924:946	bone tissue engineering	924:946	Results showed that SF-CS scaffolds are a suitable candidate for bone tissue engineering.
26235712	6	46	theme	suitable	901:908	arg1	candidate					910:918	a suitable candidate	899:918	a suitable candidate for bone tissue engineering	899:946	Results showed that SF-CS scaffolds are a suitable candidate for bone tissue engineering.
26235712	6	46	theme	suitable	901:908	arg1	scaffolds					885:893	SF-CS scaffolds	879:893	SF-CS scaffolds	879:893	Results showed that SF-CS scaffolds are a suitable candidate for bone tissue engineering.
26249571	2	0	theme	fibers	632:637	arg1	morphology					649:658	fibers and films morphology	632:658	fibers and films morphology	632:658	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	1	1	contain	having	156:161	arg1	composites					145:154	biodegradable composites	131:154	biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response	131:263	Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response has attracted great interest in order to limit/repair periodontal tissue destruction.
26249571	1	1	contain	having	156:161	arg2	ability					167:173	the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response	163:263	the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response	163:263	Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response has attracted great interest in order to limit/repair periodontal tissue destruction.
26249571	2	2	theme	anti-inflammatory	412:428	arg1	drug					430:433	non-steroidal anti-inflammatory drug	398:433	non-steroidal anti-inflammatory drug	398:433	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	4	3	theme	ultrafine	898:906	arg1	mats					916:919	drug loaded ultrafine fibrous mats	886:919	drug loaded ultrafine fibrous mats	886:919	Electrospinning was used to obtain drug loaded ultrafine fibrous mats.
26249571	2	4	theme	treatment	619:627	arg1	effect					604:609	the effect	600:609	the effect of heat treatment on fibers and films morphology	600:658	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	10	5	theme	potential	1773:1781	arg1	materials					1759:1767	these materials	1753:1767	these materials	1753:1767	The current observations suggested that these materials are potential candidates for periodontal regeneration.
26249571	10	5	theme	potential	1773:1781	arg1	candidates					1783:1792	potential candidates	1773:1792	potential candidates for periodontal regeneration	1773:1821	The current observations suggested that these materials are potential candidates for periodontal regeneration.
26249571	1	6	theme	limit/repair	306:317	arg1	destruction					338:348	limit/repair periodontal tissue destruction	306:348	limit/repair periodontal tissue destruction	306:348	Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response has attracted great interest in order to limit/repair periodontal tissue destruction.
26249571	3	7	theme	control	744:750	arg1	mats					783:786	heat treated and control (non-heat treated) nanofibrous mats	727:786	heat treated and control (non-heat treated) nanofibrous mats	727:786	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	1	8	theme	pathogenic	204:213	arg1	micro-biota					215:225	the pathogenic micro-biota	200:225	the pathogenic micro-biota	200:225	Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response has attracted great interest in order to limit/repair periodontal tissue destruction.
26249571	2	9	theme	films	643:647	arg1	morphology					649:658	fibers and films morphology	632:658	fibers and films morphology	632:658	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	8	10	dep	Control	1276:1282	arg1	mats					1334:1337	mats	1334:1337	mats	1334:1337	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	8	10	dep	Control	1276:1282	arg1	treated					1294:1300	treated	1294:1300	treated	1294:1300	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	8	10	dep	Control	1276:1282	arg1	films					1343:1347	films	1343:1347	films	1343:1347	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	2	11	theme	films	581:585	arg1	study					591:595	study	591:595	study of the effect of heat treatment on fibers and films morphology	591:658	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	11	theme	films	581:585	arg1	development					383:393	the development	379:393	the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films	379:585	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	3	12	theme	varying	809:815	arg1	concentrations					817:830	varying concentrations	809:830	varying concentrations of piroxicam (PX)	809:848	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	8	13	theme	release	1458:1464	arg1	patterns					1466:1473	burst release patterns	1452:1473	burst release patterns	1452:1473	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	8	14	theme	heat	1307:1310	arg1	fibers					1327:1332	heat treated e-spun fibers	1307:1332	heat treated e-spun fibers	1307:1332	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	9	15	theme	synthesized	1659:1669	arg1	non-cytotoxic					1698:1710	non-cytotoxic	1698:1710	non-cytotoxic	1698:1710	In vitro cytocompatibility was performed using VERO cell line of epithelial cells and all the synthesized materials were found to be non-cytotoxic.
26249571	9	15	theme	synthesized	1659:1669	arg1	materials					1671:1679	all the synthesized materials	1651:1679	all the synthesized materials	1651:1679	In vitro cytocompatibility was performed using VERO cell line of epithelial cells and all the synthesized materials were found to be non-cytotoxic.
26249571	8	16	theme	e-spun	1320:1325	arg1	fibers					1327:1332	heat treated e-spun fibers	1307:1332	heat treated e-spun fibers	1307:1332	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	2	17	theme	e-spun	542:547	arg1	mats					572:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	0	18	theme	piroxicam	13:21	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of piroxicam	0:21	Synthesis of piroxicam loaded novel electrospun biodegradable nanocomposite scaffolds for periodontal regeneration.
26249571	6	19	theme	pore	1054:1057	arg1	size					1059:1062	pore size	1054:1062	pore size	1054:1062	The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM).
26249571	2	20	theme	electro-spun	528:539	arg1	mats					572:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	6	21	dep	morphology	1028:1037	arg1	The					1024:1026	The	1024:1026	The	1024:1026	The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM).
26249571	3	22	from	films	792:796	arg1	profiles					713:720	comparative in-vitro drug release profiles	679:720	comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX)	679:848	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	0	23	theme	periodontal	90:100	arg1	regeneration					102:113	periodontal regeneration	90:113	periodontal regeneration	90:113	Synthesis of piroxicam loaded novel electrospun biodegradable nanocomposite scaffolds for periodontal regeneration.
26249571	4	24	theme	fibrous	908:914	arg1	mats					916:919	drug loaded ultrafine fibrous mats	886:919	drug loaded ultrafine fibrous mats	886:919	Electrospinning was used to obtain drug loaded ultrafine fibrous mats.
26249571	2	25	dep	drug	430:433	arg1	mats					572:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	1	26	theme	biodegradable	131:143	arg1	composites					145:154	biodegradable composites	131:154	biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response	131:263	Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response has attracted great interest in order to limit/repair periodontal tissue destruction.
26249571	7	27	theme	differential	1235:1246	arg1	calorimetry					1257:1267	differential scanning calorimetry	1235:1267	differential scanning calorimetry (DSC)	1235:1273	The thermal behavior of the materials was investigated by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC).
26249571	7	27	theme	differential	1235:1246	arg1	DSC					1270:1272	DSC	1270:1272	DSC	1270:1272	The thermal behavior of the materials was investigated by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC).
26249571	2	28	theme	composite	550:558	arg1	mats					572:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	8	29	theme	later	1520:1524	arg1	profiles					1544:1551	later sustained release profiles	1520:1551	later sustained release profiles	1520:1551	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	8	30	theme	in	1365:1366	arg1	studies					1387:1393	in vitro drug release studies	1365:1393	in vitro drug release studies	1365:1393	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	9	31	theme	In	1565:1566	arg1	cytocompatibility					1574:1590	In vitro cytocompatibility	1565:1590	In vitro cytocompatibility	1565:1590	In vitro cytocompatibility was performed using VERO cell line of epithelial cells and all the synthesized materials were found to be non-cytotoxic.
26249571	8	32	theme	release	1536:1542	arg1	profiles					1544:1551	later sustained release profiles	1520:1551	later sustained release profiles	1520:1551	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	3	33	from	mats	783:786	arg1	profiles					713:720	comparative in-vitro drug release profiles	679:720	comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX)	679:848	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	8	34	theme	drug	1374:1377	arg1	studies					1387:1393	in vitro drug release studies	1365:1393	in vitro drug release studies	1365:1393	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	2	35	theme	chitosan	468:475	arg1	mats					572:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	1	36	theme	periodontal	319:329	arg1	destruction					338:348	limit/repair periodontal tissue destruction	306:348	limit/repair periodontal tissue destruction	306:348	Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response has attracted great interest in order to limit/repair periodontal tissue destruction.
26249571	5	37	theme	Infrared	993:1000	arg1	spectroscopy					1010:1021	Infrared (FT-IR) spectroscopy	993:1021	Infrared (FT-IR) spectroscopy	993:1021	The physical/chemical interactions were evaluated by Fourier Transform Infrared (FT-IR) spectroscopy.
26249571	6	38	theme	scanning	1102:1109	arg1	SEM					1132:1134	SEM	1132:1134	SEM	1132:1134	The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM).
26249571	6	38	theme	scanning	1102:1109	arg1	microscopy					1120:1129	scanning electron microscopy	1102:1129	scanning electron microscopy (SEM)	1102:1135	The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM).
26249571	2	39	theme	vinyl	487:491	arg1	alcohol					493:499	vinyl alcohol	487:499	vinyl alcohol	487:499	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	39	theme	vinyl	487:491	arg1	chitosan					468:475	novel biodegradable chitosan	448:475	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	7	40	theme	gravimetric	1204:1214	arg1	TGA					1226:1228	TGA	1226:1228	TGA	1226:1228	The thermal behavior of the materials was investigated by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC).
26249571	7	40	theme	gravimetric	1204:1214	arg1	analysis					1216:1223	thermal gravimetric analysis	1196:1223	thermal gravimetric analysis (TGA)	1196:1229	The thermal behavior of the materials was investigated by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC).
26249571	3	41	dep	treated	732:738	arg1	treated					762:768	treated	762:768	treated	762:768	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	5	42	theme	FT-IR	1003:1007	arg1	spectroscopy					1010:1021	Infrared (FT-IR) spectroscopy	993:1021	Infrared (FT-IR) spectroscopy	993:1021	The physical/chemical interactions were evaluated by Fourier Transform Infrared (FT-IR) spectroscopy.
26249571	2	43	theme	HA	524:525	arg1	mats					572:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	3	44	theme	comparative	679:689	arg1	profiles					713:720	comparative in-vitro drug release profiles	679:720	comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX)	679:848	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	8	45	theme	physiological	1398:1410	arg1	pH7.4					1412:1416	physiological pH7.4	1398:1416	physiological pH7.4	1398:1416	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	0	46	theme	novel	30:34	arg1	scaffolds					76:84	novel electrospun biodegradable nanocomposite scaffolds	30:84	novel electrospun biodegradable nanocomposite scaffolds	30:84	Synthesis of piroxicam loaded novel electrospun biodegradable nanocomposite scaffolds for periodontal regeneration.
26249571	9	47	theme	VERO	1612:1615	arg1	line					1622:1625	VERO cell line	1612:1625	VERO cell line of epithelial cells	1612:1645	In vitro cytocompatibility was performed using VERO cell line of epithelial cells and all the synthesized materials were found to be non-cytotoxic.
26249571	6	48	theme	materials	1071:1079	arg1	structure					1040:1048	structure	1040:1048	structure	1040:1048	The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM).
26249571	6	48	theme	materials	1071:1079	arg1	size					1059:1062	pore size	1054:1062	pore size	1054:1062	The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM).
26249571	6	48	theme	materials	1071:1079	arg1	morphology					1028:1037	morphology	1028:1037	morphology	1028:1037	The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM).
26249571	2	49	theme	/hydroxyapatite	507:521	arg1	mats					572:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	50	theme	non-steroidal	398:410	arg1	drug					430:433	non-steroidal anti-inflammatory drug	398:433	non-steroidal anti-inflammatory drug	398:433	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	0	51	theme	biodegradable	48:60	arg1	scaffolds					76:84	novel electrospun biodegradable nanocomposite scaffolds	30:84	novel electrospun biodegradable nanocomposite scaffolds	30:84	Synthesis of piroxicam loaded novel electrospun biodegradable nanocomposite scaffolds for periodontal regeneration.
26249571	4	52	used	used	871:874	arg2	Electrospinning					851:865	Electrospinning	851:865	Electrospinning	851:865	Electrospinning was used to obtain drug loaded ultrafine fibrous mats.
26249571	2	53	from	morphology	649:658	arg1	study					591:595	study	591:595	study of the effect of heat treatment on fibers and films morphology	591:658	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	53	from	morphology	649:658	arg1	development					383:393	the development	379:393	the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films	379:585	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	9	54	theme	epithelial	1630:1639	arg1	cells					1641:1645	epithelial cells	1630:1645	epithelial cells	1630:1645	In vitro cytocompatibility was performed using VERO cell line of epithelial cells and all the synthesized materials were found to be non-cytotoxic.
26249571	3	55	theme	drug	700:703	arg1	profiles					713:720	comparative in-vitro drug release profiles	679:720	comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX)	679:848	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	2	56	theme	novel	448:452	arg1	PVA					503:505	PVA	503:505	PVA	503:505	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	56	theme	novel	448:452	arg1	alcohol					493:499	vinyl alcohol	487:499	vinyl alcohol	487:499	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	56	theme	novel	448:452	arg1	CS					478:479	CS	478:479	CS	478:479	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	56	theme	novel	448:452	arg1	chitosan					468:475	novel biodegradable chitosan	448:475	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	1	57	theme	great	279:283	arg1	interest					285:292	great interest	279:292	great interest	279:292	Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response has attracted great interest in order to limit/repair periodontal tissue destruction.
26249571	0	58	theme	nanocomposite	62:74	arg1	scaffolds					76:84	novel electrospun biodegradable nanocomposite scaffolds	30:84	novel electrospun biodegradable nanocomposite scaffolds	30:84	Synthesis of piroxicam loaded novel electrospun biodegradable nanocomposite scaffolds for periodontal regeneration.
26249571	2	59	theme	drug	430:433	arg1	study					591:595	study	591:595	study of the effect of heat treatment on fibers and films morphology	591:658	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	59	theme	drug	430:433	arg1	development					383:393	the development	379:393	the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films	379:585	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	4	60	theme	loaded	891:896	arg1	mats					916:919	drug loaded ultrafine fibrous mats	886:919	drug loaded ultrafine fibrous mats	886:919	Electrospinning was used to obtain drug loaded ultrafine fibrous mats.
26249571	2	61	from	development	383:393	arg1	morphology					649:658	fibers and films morphology	632:658	fibers and films morphology	632:658	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	7	62	theme	thermal	1142:1148	arg1	behavior					1150:1157	The thermal behavior	1138:1157	The thermal behavior of the materials	1138:1174	The thermal behavior of the materials was investigated by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC).
26249571	2	63	theme	heat	614:617	arg1	treatment					619:627	heat treatment	614:627	heat treatment	614:627	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	64	from	effect	604:609	arg1	morphology					649:658	fibers and films morphology	632:658	fibers and films morphology	632:658	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	10	65	theme	current	1717:1723	arg1	observations					1725:1736	The current observations	1713:1736	The current observations	1713:1736	The current observations suggested that these materials are potential candidates for periodontal regeneration.
26249571	2	66	theme	present	355:361	arg1	report					363:368	The present report	351:368	The present report	351:368	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	8	67	dep	in	1365:1366	arg1	vitro					1368:1372	vitro	1368:1372	vitro	1368:1372	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	4	68	theme	drug	886:889	arg1	mats					916:919	drug loaded ultrafine fibrous mats	886:919	drug loaded ultrafine fibrous mats	886:919	Electrospinning was used to obtain drug loaded ultrafine fibrous mats.
26249571	8	69	theme	burst	1452:1456	arg1	patterns					1466:1473	burst release patterns	1452:1473	burst release patterns	1452:1473	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	3	70	theme	nanofibrous	771:781	arg1	mats					783:786	heat treated and control (non-heat treated) nanofibrous mats	727:786	heat treated and control (non-heat treated) nanofibrous mats	727:786	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	1	71	theme	inflammatory	243:254	arg1	response					256:263	the inflammatory response	239:263	the inflammatory response	239:263	Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response has attracted great interest in order to limit/repair periodontal tissue destruction.
26249571	1	72	theme	composites	145:154	arg1	Development					116:126	Development	116:126	Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response	116:263	Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response has attracted great interest in order to limit/repair periodontal tissue destruction.
26249571	2	73	theme	effect	604:609	arg1	study					591:595	study	591:595	study of the effect of heat treatment on fibers and films morphology	591:658	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	73	theme	effect	604:609	arg1	development					383:393	the development	379:393	the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films	379:585	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	9	74	dep	In	1565:1566	arg1	vitro					1568:1572	vitro	1568:1572	vitro	1568:1572	In vitro cytocompatibility was performed using VERO cell line of epithelial cells and all the synthesized materials were found to be non-cytotoxic.
26249571	10	75	theme	periodontal	1798:1808	arg1	regeneration					1810:1821	periodontal regeneration	1798:1821	periodontal regeneration	1798:1821	The current observations suggested that these materials are potential candidates for periodontal regeneration.
26249571	3	76	contain	containing	798:807	arg2	concentrations					817:830	varying concentrations	809:830	varying concentrations of piroxicam (PX)	809:848	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	3	76	contain	containing	798:807	arg1	films					792:796	films	792:796	films containing varying concentrations of piroxicam (PX)	792:848	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	8	77	theme	treated	1312:1318	arg1	fibers					1327:1332	heat treated e-spun fibers	1307:1332	heat treated e-spun fibers	1307:1332	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	3	78	theme	piroxicam	835:843	arg1	concentrations					817:830	varying concentrations	809:830	varying concentrations of piroxicam (PX)	809:848	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	7	79	theme	scanning	1248:1255	arg1	calorimetry					1257:1267	differential scanning calorimetry	1235:1267	differential scanning calorimetry (DSC)	1235:1273	The thermal behavior of the materials was investigated by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC).
26249571	7	79	theme	scanning	1248:1255	arg1	DSC					1270:1272	DSC	1270:1272	DSC	1270:1272	The thermal behavior of the materials was investigated by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC).
26249571	5	80	theme	physical/chemical	926:942	arg1	interactions					944:955	The physical/chemical interactions	922:955	The physical/chemical interactions	922:955	The physical/chemical interactions were evaluated by Fourier Transform Infrared (FT-IR) spectroscopy.
26249571	2	81	theme	nanofibrous	560:570	arg1	mats					572:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	8	82	theme	sustained	1526:1534	arg1	profiles					1544:1551	later sustained release profiles	1520:1551	later sustained release profiles	1520:1551	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	5	83	dep	Fourier	975:981	arg1	Transform					983:991	Transform	983:991	Transform Infrared (FT-IR) spectroscopy	983:1021	The physical/chemical interactions were evaluated by Fourier Transform Infrared (FT-IR) spectroscopy.
26249571	6	84	theme	electron	1111:1118	arg1	SEM					1132:1134	SEM	1132:1134	SEM	1132:1134	The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM).
26249571	6	84	theme	electron	1111:1118	arg1	microscopy					1120:1129	scanning electron microscopy	1102:1129	scanning electron microscopy (SEM)	1102:1135	The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM).
26249571	2	85	theme	biodegradable	454:466	arg1	PVA					503:505	PVA	503:505	PVA	503:505	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	85	theme	biodegradable	454:466	arg1	alcohol					493:499	vinyl alcohol	487:499	vinyl alcohol	487:499	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	85	theme	biodegradable	454:466	arg1	CS					478:479	CS	478:479	CS	478:479	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	2	85	theme	biodegradable	454:466	arg1	chitosan					468:475	novel biodegradable chitosan	448:475	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	7	86	theme	thermal	1196:1202	arg1	TGA					1226:1228	TGA	1226:1228	TGA	1226:1228	The thermal behavior of the materials was investigated by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC).
26249571	7	86	theme	thermal	1196:1202	arg1	analysis					1216:1223	thermal gravimetric analysis	1196:1223	thermal gravimetric analysis (TGA)	1196:1229	The thermal behavior of the materials was investigated by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC).
26249571	1	87	theme	tissue	331:336	arg1	destruction					338:348	limit/repair periodontal tissue destruction	306:348	limit/repair periodontal tissue destruction	306:348	Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response has attracted great interest in order to limit/repair periodontal tissue destruction.
26249571	8	88	theme	release	1379:1385	arg1	studies					1387:1393	in vitro drug release studies	1365:1393	in vitro drug release studies	1365:1393	Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted.
26249571	0	89	theme	electrospun	36:46	arg1	scaffolds					76:84	novel electrospun biodegradable nanocomposite scaffolds	30:84	novel electrospun biodegradable nanocomposite scaffolds	30:84	Synthesis of piroxicam loaded novel electrospun biodegradable nanocomposite scaffolds for periodontal regeneration.
26249571	7	90	theme	materials	1166:1174	arg1	behavior					1150:1157	The thermal behavior	1138:1157	The thermal behavior of the materials	1138:1174	The thermal behavior of the materials was investigated by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC).
26249571	9	91	theme	cell	1617:1620	arg1	line					1622:1625	VERO cell line	1612:1625	VERO cell line of epithelial cells	1612:1645	In vitro cytocompatibility was performed using VERO cell line of epithelial cells and all the synthesized materials were found to be non-cytotoxic.
26249571	3	92	theme	release	705:711	arg1	profiles					713:720	comparative in-vitro drug release profiles	679:720	comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX)	679:848	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	3	93	theme	in-vitro	691:698	arg1	profiles					713:720	comparative in-vitro drug release profiles	679:720	comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX)	679:848	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	9	94	theme	cells	1641:1645	arg1	line					1622:1625	VERO cell line	1612:1625	VERO cell line of epithelial cells	1612:1645	In vitro cytocompatibility was performed using VERO cell line of epithelial cells and all the synthesized materials were found to be non-cytotoxic.
26249571	2	95	from	study	591:595	arg1	morphology					649:658	fibers and films morphology	632:658	fibers and films morphology	632:658	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
26249571	3	96	theme	treated	732:738	arg1	mats					783:786	heat treated and control (non-heat treated) nanofibrous mats	727:786	heat treated and control (non-heat treated) nanofibrous mats	727:786	It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX).
26249571	2	97	theme	encapsulated	435:446	arg1	mats					572:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats	435:575	The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology.
25037384	3	0	theme	ELSP	325:328	arg1	technique					331:339	an electrospinning (ELSP) technique	305:339	an electrospinning (ELSP) technique	305:339	In this study, we utilized an electrospinning (ELSP) technique to design a novel wound dressing.
25037384	2	1	theme	ideal	142:146	arg1	dressing					154:161	The ideal wound dressing	138:161	The ideal wound dressing	138:161	The ideal wound dressing would have properties that allow for absorption of exudates, and inhibition of microorganism for wound protection.
25037384	6	2	attach	presence	580:587	arg2	AgNPs					592:596	AgNPs	592:596	AgNPs	592:596	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	6	2	attach	presence	580:587	arg1	composite					615:623	the CTS/AgNPs composite	601:623	the CTS/AgNPs composite	601:623	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	2	3	contain	have	169:172	arg2	properties					174:183	properties	174:183	properties that allow for absorption of exudates	174:221	The ideal wound dressing would have properties that allow for absorption of exudates, and inhibition of microorganism for wound protection.
25037384	2	3	contain	have	169:172	arg2	inhibition					228:237	inhibition	228:237	inhibition of microorganism	228:254	The ideal wound dressing would have properties that allow for absorption of exudates, and inhibition of microorganism for wound protection.
25037384	2	3	contain	have	169:172	arg1	dressing					154:161	The ideal wound dressing	138:161	The ideal wound dressing	138:161	The ideal wound dressing would have properties that allow for absorption of exudates, and inhibition of microorganism for wound protection.
25037384	6	4	theme	CTS/AgNPs	605:613	arg1	composite					615:623	the CTS/AgNPs composite	601:623	the CTS/AgNPs composite	601:623	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	9	5	theme	topical	1203:1209	arg1	treatment					1225:1233	topical antibacterial treatment	1203:1233	topical antibacterial treatment in wound care	1203:1247	Results of this antibacterial testing suggest that CTS/AgNPs nanofibers may be effective in topical antibacterial treatment in wound care.
25037384	4	6	theme	silver	430:435	arg1	AgNPs					452:456	AgNPs	452:456	AgNPs	452:456	Chitosan (CTS) nanofibers containing various ratios of silver nanoparticles (AgNPs) were obtained.
25037384	4	6	theme	silver	430:435	arg1	nanoparticles					437:449	silver nanoparticles	430:449	silver nanoparticles (AgNPs)	430:457	Chitosan (CTS) nanofibers containing various ratios of silver nanoparticles (AgNPs) were obtained.
25037384	9	7	theme	antibacterial	1211:1223	arg1	treatment					1225:1233	topical antibacterial treatment	1203:1233	topical antibacterial treatment in wound care	1203:1247	Results of this antibacterial testing suggest that CTS/AgNPs nanofibers may be effective in topical antibacterial treatment in wound care.
25037384	9	8	from	treatment	1225:1233	arg1	effective					1190:1198	effective	1190:1198	effective	1190:1198	Results of this antibacterial testing suggest that CTS/AgNPs nanofibers may be effective in topical antibacterial treatment in wound care.
25037384	9	8	from	treatment	1225:1233	arg1	care					1244:1247	wound care	1238:1247	wound care	1238:1247	Results of this antibacterial testing suggest that CTS/AgNPs nanofibers may be effective in topical antibacterial treatment in wound care.
25037384	7	9	theme	transmission	857:868	arg1	microscopy					879:888	transmission electron microscopy	857:888	transmission electron microscopy (TEM)	857:894	The electrospun CTS/AgNPs nanofibers were characterized morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
25037384	7	9	theme	transmission	857:868	arg1	TEM					891:893	TEM	891:893	TEM	891:893	The electrospun CTS/AgNPs nanofibers were characterized morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
25037384	3	10	theme	novel	353:357	arg1	dressing					365:372	a novel wound dressing	351:372	a novel wound dressing	351:372	In this study, we utilized an electrospinning (ELSP) technique to design a novel wound dressing.
25037384	4	11	theme	CTS	385:387	arg1	nanofibers					390:399	Chitosan (CTS) nanofibers	375:399	Chitosan (CTS) nanofibers containing various ratios of silver nanoparticles (AgNPs)	375:457	Chitosan (CTS) nanofibers containing various ratios of silver nanoparticles (AgNPs) were obtained.
25037384	7	12	theme	electron	870:877	arg1	microscopy					879:888	transmission electron microscopy	857:888	transmission electron microscopy (TEM)	857:894	The electrospun CTS/AgNPs nanofibers were characterized morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
25037384	7	12	theme	electron	870:877	arg1	TEM					891:893	TEM	891:893	TEM	891:893	The electrospun CTS/AgNPs nanofibers were characterized morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
25037384	3	13	theme	wound	359:363	arg1	dressing					365:372	a novel wound dressing	351:372	a novel wound dressing	351:372	In this study, we utilized an electrospinning (ELSP) technique to design a novel wound dressing.
25037384	3	14	used	utilized	296:303	arg2	we					293:294	we	293:294	we	293:294	In this study, we utilized an electrospinning (ELSP) technique to design a novel wound dressing.
25037384	9	15	theme	wound	1238:1242	arg1	care					1244:1247	wound care	1238:1247	wound care	1238:1247	Results of this antibacterial testing suggest that CTS/AgNPs nanofibers may be effective in topical antibacterial treatment in wound care.
25037384	6	16	theme	ultraviolet-visible	667:685	arg1	UV					701:702	UV	701:702	UV	701:702	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	6	16	theme	ultraviolet-visible	667:685	arg1	spectroscopy					687:698	ultraviolet-visible spectroscopy	667:698	ultraviolet-visible spectroscopy (UV)	667:703	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	6	17	theme	AgNPs	592:596	arg1	presence					580:587	presence	580:587	presence	580:587	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	6	17	theme	AgNPs	592:596	arg1	formation					566:574	formation	566:574	formation	566:574	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	8	18	dep	aeruginosa	1014:1023	arg1	aeruginosa					1029:1038	P. aeruginosa	1026:1038	P. aeruginosa	1026:1038	These nanofibers were subsequently tested to evaluate their antibacterial activity against gram-negative Pseudomonas aeruginosa (P. aeruginosa) and gram-positive Methicillin-resistant Staphylococcus aureus (MRSA).
25037384	0	19	theme	Electrospun	0:10	arg1	chitosan					12:19	Electrospun chitosan	0:19	Electrospun chitosan	0:19	Electrospun chitosan nanofibers with controlled levels of silver nanoparticles.
25037384	6	20	from	formation	566:574	arg1	composite					615:623	the CTS/AgNPs composite	601:623	the CTS/AgNPs composite	601:623	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	7	21	theme	electron	827:834	arg1	SEM					848:850	SEM	848:850	SEM	848:850	The electrospun CTS/AgNPs nanofibers were characterized morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
25037384	7	21	theme	electron	827:834	arg1	microscopy					836:845	scanning electron microscopy	818:845	scanning electron microscopy (SEM)	818:851	The electrospun CTS/AgNPs nanofibers were characterized morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
25037384	9	22	from	effective	1190:1198	arg1	treatment					1225:1233	topical antibacterial treatment	1203:1233	topical antibacterial treatment in wound care	1203:1247	Results of this antibacterial testing suggest that CTS/AgNPs nanofibers may be effective in topical antibacterial treatment in wound care.
25037384	4	23	theme	Chitosan	375:382	arg1	nanofibers					390:399	Chitosan (CTS) nanofibers	375:399	Chitosan (CTS) nanofibers containing various ratios of silver nanoparticles (AgNPs)	375:457	Chitosan (CTS) nanofibers containing various ratios of silver nanoparticles (AgNPs) were obtained.
25037384	1	24	theme	antibacterial	114:126	arg1	activity					128:135	antibacterial activity	114:135	antibacterial activity	114:135	Preparation, characterization and antibacterial activity.
25037384	9	25	theme	antibacterial	1127:1139	arg1	testing					1141:1147	this antibacterial testing	1122:1147	this antibacterial testing	1122:1147	Results of this antibacterial testing suggest that CTS/AgNPs nanofibers may be effective in topical antibacterial treatment in wound care.
25037384	6	26	from	presence	580:587	arg1	composite					615:623	the CTS/AgNPs composite	601:623	the CTS/AgNPs composite	601:623	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	9	27	theme	testing	1141:1147	arg1	Results					1111:1117	Results	1111:1117	Results of this antibacterial testing	1111:1147	Results of this antibacterial testing suggest that CTS/AgNPs nanofibers may be effective in topical antibacterial treatment in wound care.
25037384	6	28	dep	formation	566:574	arg1	The					562:564	The	562:564	The	562:564	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	4	29	theme	nanoparticles	437:449	arg1	ratios					420:425	various ratios	412:425	various ratios of silver nanoparticles (AgNPs)	412:457	Chitosan (CTS) nanofibers containing various ratios of silver nanoparticles (AgNPs) were obtained.
25037384	7	30	theme	scanning	818:825	arg1	SEM					848:850	SEM	848:850	SEM	848:850	The electrospun CTS/AgNPs nanofibers were characterized morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
25037384	7	30	theme	scanning	818:825	arg1	microscopy					836:845	scanning electron microscopy	818:845	scanning electron microscopy (SEM)	818:851	The electrospun CTS/AgNPs nanofibers were characterized morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
25037384	4	31	contain	containing	401:410	arg1	nanofibers					390:399	Chitosan (CTS) nanofibers	375:399	Chitosan (CTS) nanofibers containing various ratios of silver nanoparticles (AgNPs)	375:457	Chitosan (CTS) nanofibers containing various ratios of silver nanoparticles (AgNPs) were obtained.
25037384	4	31	contain	containing	401:410	arg2	ratios					420:425	various ratios	412:425	various ratios of silver nanoparticles (AgNPs)	412:457	Chitosan (CTS) nanofibers containing various ratios of silver nanoparticles (AgNPs) were obtained.
25037384	7	32	theme	electrospun	747:757	arg1	nanofibers					769:778	The electrospun CTS/AgNPs nanofibers	743:778	The electrospun CTS/AgNPs nanofibers	743:778	The electrospun CTS/AgNPs nanofibers were characterized morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
25037384	0	33	theme	controlled	37:46	arg1	levels					48:53	controlled levels	37:53	controlled levels of silver nanoparticles	37:77	Electrospun chitosan nanofibers with controlled levels of silver nanoparticles.
25037384	2	34	theme	microorganism	242:254	arg1	inhibition					228:237	inhibition	228:237	inhibition of microorganism	228:254	The ideal wound dressing would have properties that allow for absorption of exudates, and inhibition of microorganism for wound protection.
25037384	2	34	theme	microorganism	242:254	arg1	properties					174:183	properties	174:183	properties that allow for absorption of exudates	174:221	The ideal wound dressing would have properties that allow for absorption of exudates, and inhibition of microorganism for wound protection.
25037384	0	35	theme	silver	58:63	arg1	nanoparticles					65:77	silver nanoparticles	58:77	silver nanoparticles	58:77	Electrospun chitosan nanofibers with controlled levels of silver nanoparticles.
25037384	7	36	theme	CTS/AgNPs	759:767	arg1	nanofibers					769:778	The electrospun CTS/AgNPs nanofibers	743:778	The electrospun CTS/AgNPs nanofibers	743:778	The electrospun CTS/AgNPs nanofibers were characterized morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
25037384	3	37	theme	electrospinning	308:322	arg1	technique					331:339	an electrospinning (ELSP) technique	305:339	an electrospinning (ELSP) technique	305:339	In this study, we utilized an electrospinning (ELSP) technique to design a novel wound dressing.
25037384	9	38	theme	CTS/AgNPs	1162:1170	arg1	nanofibers					1172:1181	CTS/AgNPs nanofibers	1162:1181	CTS/AgNPs nanofibers	1162:1181	Results of this antibacterial testing suggest that CTS/AgNPs nanofibers may be effective in topical antibacterial treatment in wound care.
25037384	5	39	theme	chemical	535:542	arg1	method					554:559	a chemical reduction method	533:559	a chemical reduction method	533:559	AgNPs were generated directly in the CTS solution by using a chemical reduction method.
25037384	6	40	theme	x-ray	642:646	arg1	XRD					661:663	XRD	661:663	XRD	661:663	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	6	40	theme	x-ray	642:646	arg1	diffraction					648:658	x-ray diffraction	642:658	x-ray diffraction (XRD)	642:664	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	4	41	theme	various	412:418	arg1	ratios					420:425	various ratios	412:425	various ratios of silver nanoparticles (AgNPs)	412:457	Chitosan (CTS) nanofibers containing various ratios of silver nanoparticles (AgNPs) were obtained.
25037384	5	42	theme	reduction	544:552	arg1	method					554:559	a chemical reduction method	533:559	a chemical reduction method	533:559	AgNPs were generated directly in the CTS solution by using a chemical reduction method.
25037384	2	43	theme	exudates	214:221	arg1	absorption					200:209	absorption	200:209	absorption of exudates	200:221	The ideal wound dressing would have properties that allow for absorption of exudates, and inhibition of microorganism for wound protection.
25037384	6	44	theme	thermogravimetric	709:725	arg1	TGA					737:739	TGA	737:739	TGA	737:739	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	6	44	theme	thermogravimetric	709:725	arg1	analysis					727:734	thermogravimetric analysis	709:734	thermogravimetric analysis (TGA)	709:740	The formation and presence of AgNPs in the CTS/AgNPs composite was confirmed by x-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV) and thermogravimetric analysis (TGA).
25037384	5	45	theme	CTS	511:513	arg1	solution					515:522	the CTS solution	507:522	the CTS solution	507:522	AgNPs were generated directly in the CTS solution by using a chemical reduction method.
25037384	0	46	theme	nanoparticles	65:77	arg1	levels					48:53	controlled levels	37:53	controlled levels of silver nanoparticles	37:77	Electrospun chitosan nanofibers with controlled levels of silver nanoparticles.
25037384	2	47	theme	wound	148:152	arg1	dressing					154:161	The ideal wound dressing	138:161	The ideal wound dressing	138:161	The ideal wound dressing would have properties that allow for absorption of exudates, and inhibition of microorganism for wound protection.
25037384	2	48	theme	wound	260:264	arg1	protection					266:275	wound protection	260:275	wound protection	260:275	The ideal wound dressing would have properties that allow for absorption of exudates, and inhibition of microorganism for wound protection.
25037384	8	49	theme	antibacterial	957:969	arg1	activity					971:978	their antibacterial activity	951:978	their antibacterial activity against gram-negative Pseudomonas aeruginosa (P. aeruginosa) and gram-positive Methicillin-resistant Staphylococcus aureus (MRSA)	951:1108	These nanofibers were subsequently tested to evaluate their antibacterial activity against gram-negative Pseudomonas aeruginosa (P. aeruginosa) and gram-positive Methicillin-resistant Staphylococcus aureus (MRSA).
26428179	4	0	theme	co-existing	682:692	arg1	anions					694:699	co-existing anions	682:699	co-existing anions	682:699	The defluoridation capacity (DC) was found to depend on the contact time, pH, co-existing anions, initial fluoride concentration and temperature.
26428179	7	1	theme	easy	1159:1162	arg1	separation					1173:1182	the easy magnetic separation	1155:1182	the easy magnetic separation of Fe3O4@n-HApCS	1155:1199	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	1	2	from	pollutants	212:221	arg1	environment					238:248	the aqueous environment	226:248	the aqueous environment	226:248	Adsorption is a significant reaction occurs between adsorbent/water interface for controlling the pollutants in the aqueous environment.
26428179	2	3	theme	n-HAp	396:400	arg1	nanocomposite					417:429	nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite	375:429	nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption	375:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	4	4	theme	defluoridation	608:621	arg1	capacity					623:630	The defluoridation capacity	604:630	The defluoridation capacity (DC)	604:635	The defluoridation capacity (DC) was found to depend on the contact time, pH, co-existing anions, initial fluoride concentration and temperature.
26428179	4	4	theme	defluoridation	608:621	arg1	DC					633:634	DC	633:634	DC	633:634	The defluoridation capacity (DC) was found to depend on the contact time, pH, co-existing anions, initial fluoride concentration and temperature.
26428179	0	5	theme	fluoride	95:102	arg1	sorption					104:111	enhanced fluoride sorption	86:111	enhanced fluoride sorption	86:111	Synthesis and applications of eco-magnetic nano-hydroxyapatite chitosan composite for enhanced fluoride sorption.
26428179	6	6	theme	endothermic	935:945	arg1	nature					947:952	endothermic nature	935:952	endothermic nature	935:952	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.
26428179	7	7	theme	magnetic	1164:1171	arg1	separation					1173:1182	the easy magnetic separation	1155:1182	the easy magnetic separation of Fe3O4@n-HApCS	1155:1199	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	7	8	theme	research	996:1003	arg1	results					980:986	The results	976:986	The results of this research	976:1003	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	3	9	theme	EDAX	587:590	arg1	techniques					592:601	EDAX techniques	587:601	EDAX techniques	587:601	The resulting Fe3O4@n-HApCS nanocomposite was characterized by FTIR and SEM with EDAX techniques.
26428179	7	10	theme	Fe3O4	1187:1191	arg1	n-HApCS					1193:1199	Fe3O4@n-HApCS	1187:1199	Fe3O4@n-HApCS	1187:1199	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	3	11	theme	n-HApCS	526:532	arg1	nanocomposite					534:546	The resulting Fe3O4@n-HApCS nanocomposite	506:546	The resulting Fe3O4@n-HApCS nanocomposite	506:546	The resulting Fe3O4@n-HApCS nanocomposite was characterized by FTIR and SEM with EDAX techniques.
26428179	2	12	theme	nano-hydroxyapatite	375:393	arg1	nanocomposite					417:429	nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite	375:429	nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption	375:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	5	13	theme	batch	850:854	arg1	method					856:861	the batch method	846:861	the batch method	846:861	The sorption isotherm was investigated by Freundlich, Langmuir and Temkin isotherm models using the batch method.
26428179	2	14	theme	@	443:443	arg1	n-HApCS					444:450	Fe3O4@n-HApCS	438:450	nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption	375:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	2	15	theme	uniform	310:316	arg1	deposition					318:327	uniform deposition	310:327	uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption	310:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	2	16	theme	nanocomposite	417:429	arg1	surface					364:370	the surface	360:370	the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption	360:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	3	17	with	SEM	578:580	arg1	techniques					592:601	EDAX techniques	587:601	EDAX techniques	587:601	The resulting Fe3O4@n-HApCS nanocomposite was characterized by FTIR and SEM with EDAX techniques.
26428179	5	18	theme	Freundlich	792:801	arg1	models					833:838	Freundlich, Langmuir and Temkin isotherm models	792:838	models	833:838	The sorption isotherm was investigated by Freundlich, Langmuir and Temkin isotherm models using the batch method.
26428179	1	19	dep	reaction	142:149	arg1	occurs					151:156	occurs	151:156	occurs	151:156	Adsorption is a significant reaction occurs between adsorbent/water interface for controlling the pollutants in the aqueous environment.
26428179	3	20	theme	Fe3O4	520:524	arg1	nanocomposite					534:546	The resulting Fe3O4@n-HApCS nanocomposite	506:546	The resulting Fe3O4@n-HApCS nanocomposite	506:546	The resulting Fe3O4@n-HApCS nanocomposite was characterized by FTIR and SEM with EDAX techniques.
26428179	4	21	theme	contact	664:670	arg1	time					672:675	the contact time	660:675	the contact time	660:675	The defluoridation capacity (DC) was found to depend on the contact time, pH, co-existing anions, initial fluoride concentration and temperature.
26428179	5	22	theme	Temkin	817:822	arg1	models					833:838	Freundlich, Langmuir and Temkin isotherm models	792:838	models	833:838	The sorption isotherm was investigated by Freundlich, Langmuir and Temkin isotherm models using the batch method.
26428179	2	23	theme	CS	413:414	arg1	nanocomposite					417:429	nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite	375:429	nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption	375:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	2	24	theme	versatile	465:473	arg1	sorbent					475:481	versatile sorbent	465:481	versatile sorbent for fluoride sorption	465:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	7	25	theme	@	1192:1192	arg1	n-HApCS					1193:1199	Fe3O4@n-HApCS	1187:1199	Fe3O4@n-HApCS	1187:1199	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	2	26	theme	particles	347:355	arg1	deposition					318:327	uniform deposition	310:327	uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption	310:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	7	27	dep	work	1005:1008	arg1	designated					1010:1019	designated	1010:1019	work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium	1005:1229	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	7	28	dep	composite	1201:1209	arg1	separation					1173:1182	the easy magnetic separation	1155:1182	the easy magnetic separation of Fe3O4@n-HApCS	1155:1199	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	7	29	theme	n-HApCS	1193:1199	arg1	separation					1173:1182	the easy magnetic separation	1155:1182	the easy magnetic separation of Fe3O4@n-HApCS	1155:1199	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	5	30	theme	isotherm	824:831	arg1	models					833:838	Freundlich, Langmuir and Temkin isotherm models	792:838	models	833:838	The sorption isotherm was investigated by Freundlich, Langmuir and Temkin isotherm models using the batch method.
26428179	7	31	theme	individual	1104:1113	arg1	components					1115:1124	the individual components	1100:1124	the individual components	1100:1124	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	2	32	theme	Fe3O4	341:345	arg1	particles					347:355	magnetic Fe3O4 particles	332:355	magnetic Fe3O4 particles	332:355	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	7	33	dep	designated	1010:1019	arg1	composite					1040:1048	Fe3O4@n-HApCS composite	1026:1048	Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium	1026:1229	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	1	34	theme	aqueous	230:236	arg1	environment					238:248	the aqueous environment	226:248	the aqueous environment	226:248	Adsorption is a significant reaction occurs between adsorbent/water interface for controlling the pollutants in the aqueous environment.
26428179	7	35	from	medium	1224:1229	arg1	composite					1201:1209	composite	1201:1209	composite	1201:1209	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	2	36	theme	magnetic	332:339	arg1	particles					347:355	magnetic Fe3O4 particles	332:355	magnetic Fe3O4 particles	332:355	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	1	37	theme	adsorbent/water	166:180	arg1	interface					182:190	adsorbent/water interface	166:190	adsorbent/water interface for controlling the pollutants in the aqueous environment	166:248	Adsorption is a significant reaction occurs between adsorbent/water interface for controlling the pollutants in the aqueous environment.
26428179	0	38	theme	nano-hydroxyapatite	43:61	arg1	chitosan					63:70	eco-magnetic nano-hydroxyapatite chitosan	30:70	eco-magnetic nano-hydroxyapatite chitosan	30:70	Synthesis and applications of eco-magnetic nano-hydroxyapatite chitosan composite for enhanced fluoride sorption.
26428179	4	39	theme	initial	702:708	arg1	concentration					719:731	initial fluoride concentration	702:731	initial fluoride concentration	702:731	The defluoridation capacity (DC) was found to depend on the contact time, pH, co-existing anions, initial fluoride concentration and temperature.
26428179	7	40	theme	aqueous	1216:1222	arg1	medium					1224:1229	aqueous medium	1216:1229	aqueous medium	1216:1229	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	2	41	theme	Fe3O4	438:442	arg1	n-HApCS					444:450	Fe3O4@n-HApCS	438:450	nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption	375:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	0	42	theme	eco-magnetic	30:41	arg1	chitosan					63:70	eco-magnetic nano-hydroxyapatite chitosan	30:70	eco-magnetic nano-hydroxyapatite chitosan	30:70	Synthesis and applications of eco-magnetic nano-hydroxyapatite chitosan composite for enhanced fluoride sorption.
26428179	3	43	with	FTIR	569:572	arg1	techniques					592:601	EDAX techniques	587:601	EDAX techniques	587:601	The resulting Fe3O4@n-HApCS nanocomposite was characterized by FTIR and SEM with EDAX techniques.
26428179	6	44	theme	thermodynamic	868:880	arg1	parameters					882:891	The thermodynamic parameters	864:891	The thermodynamic parameters	864:891	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.
26428179	5	45	theme	sorption	754:761	arg1	isotherm					763:770	The sorption isotherm	750:770	The sorption isotherm	750:770	The sorption isotherm was investigated by Freundlich, Langmuir and Temkin isotherm models using the batch method.
26428179	2	46	theme	fluoride	487:494	arg1	sorption					496:503	fluoride sorption	487:503	fluoride sorption	487:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	7	47	theme	defluoridation	1071:1084	arg1	capacity					1086:1093	the excellent defluoridation capacity	1057:1093	the excellent defluoridation capacity than the individual components	1057:1124	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	1	48	theme	significant	130:140	arg1	Adsorption					114:123	Adsorption	114:123	Adsorption	114:123	Adsorption is a significant reaction occurs between adsorbent/water interface for controlling the pollutants in the aqueous environment.
26428179	1	48	theme	significant	130:140	arg1	reaction					142:149	a significant reaction occurs	128:156	a significant reaction occurs between adsorbent/water interface for controlling the pollutants in the aqueous environment	128:248	Adsorption is a significant reaction occurs between adsorbent/water interface for controlling the pollutants in the aqueous environment.
26428179	0	49	theme	chitosan	63:70	arg1	applications					14:25	applications	14:25	applications	14:25	Synthesis and applications of eco-magnetic nano-hydroxyapatite chitosan composite for enhanced fluoride sorption.
26428179	0	49	theme	chitosan	63:70	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and applications of eco-magnetic nano-hydroxyapatite chitosan composite for enhanced fluoride sorption.
26428179	6	50	theme	sorption	966:973	arg1	spontaneity					919:929	spontaneity	919:929	spontaneity	919:929	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.
26428179	6	50	theme	sorption	966:973	arg1	nature					947:952	endothermic nature	935:952	endothermic nature	935:952	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.
26428179	6	50	theme	sorption	966:973	arg1	feasibility					906:916	feasibility	906:916	feasibility	906:916	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.
26428179	7	51	theme	@	1031:1031	arg1	composite					1040:1048	Fe3O4@n-HApCS composite	1026:1048	Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium	1026:1229	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	3	52	theme	@	525:525	arg1	nanocomposite					534:546	The resulting Fe3O4@n-HApCS nanocomposite	506:546	The resulting Fe3O4@n-HApCS nanocomposite	506:546	The resulting Fe3O4@n-HApCS nanocomposite was characterized by FTIR and SEM with EDAX techniques.
26428179	6	53	theme	fluoride	957:964	arg1	sorption					966:973	fluoride sorption	957:973	fluoride sorption	957:973	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.
26428179	4	54	theme	fluoride	710:717	arg1	concentration					719:731	initial fluoride concentration	702:731	initial fluoride concentration	702:731	The defluoridation capacity (DC) was found to depend on the contact time, pH, co-existing anions, initial fluoride concentration and temperature.
26428179	7	55	theme	n-HApCS	1032:1038	arg1	composite					1040:1048	Fe3O4@n-HApCS composite	1026:1048	Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium	1026:1229	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	7	56	theme	excellent	1061:1069	arg1	capacity					1086:1093	the excellent defluoridation capacity	1057:1093	the excellent defluoridation capacity than the individual components	1057:1124	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	3	57	theme	resulting	510:518	arg1	nanocomposite					534:546	The resulting Fe3O4@n-HApCS nanocomposite	506:546	The resulting Fe3O4@n-HApCS nanocomposite	506:546	The resulting Fe3O4@n-HApCS nanocomposite was characterized by FTIR and SEM with EDAX techniques.
26428179	7	58	dep	composite	1040:1048	arg1	interesting					1130:1140	interesting	1130:1140	interesting	1130:1140	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	7	58	dep	composite	1040:1048	arg1	having					1050:1055	having	1050:1055	having the excellent defluoridation capacity than the individual components	1050:1124	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	0	59	theme	enhanced	86:93	arg1	sorption					104:111	enhanced fluoride sorption	86:111	enhanced fluoride sorption	86:111	Synthesis and applications of eco-magnetic nano-hydroxyapatite chitosan composite for enhanced fluoride sorption.
26428179	2	60	dep	nanocomposite	417:429	arg1	n-HApCS					444:450	Fe3O4@n-HApCS	438:450	nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption	375:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	7	61	theme	Fe3O4	1026:1030	arg1	composite					1040:1048	Fe3O4@n-HApCS composite	1026:1048	Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium	1026:1229	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
26428179	2	62	theme	/chitosan	402:410	arg1	nanocomposite					417:429	nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite	375:429	nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption	375:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	2	63	from	deposition	318:327	arg1	surface					364:370	the surface	360:370	the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption	360:503	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	5	64	theme	Langmuir	804:811	arg1	models					833:838	Freundlich, Langmuir and Temkin isotherm models	792:838	models	833:838	The sorption isotherm was investigated by Freundlich, Langmuir and Temkin isotherm models using the batch method.
26428179	2	65	theme	eco-magnetic	270:281	arg1	biosorbent					283:292	an eco-magnetic biosorbent	267:292	an eco-magnetic biosorbent	267:292	In this regard, an eco-magnetic biosorbent was prepared by uniform deposition of magnetic Fe3O4 particles on the surface of nano-hydroxyapatite (n-HAp)/chitosan (CS) nanocomposite namely Fe3O4@n-HApCS composite as versatile sorbent for fluoride sorption.
26428179	7	66	dep	note	1145:1148	arg1	composite					1201:1209	composite	1201:1209	composite	1201:1209	The results of this research work designated that Fe3O4@n-HApCS composite having the excellent defluoridation capacity than the individual components and interesting to note that the easy magnetic separation of Fe3O4@n-HApCS composite from aqueous medium.
29055865	8	0	theme	controlled	992:1001	arg1	release					1003:1009	controlled release	992:1009	controlled release	992:1009	In vitro DFO release from the prepared composite hydrogels showed controlled release over a period of 10days.
29055865	4	1	theme	chitosan-hyaluronic	620:638	arg1	hydrogel					664:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel was prepared.
29055865	11	2	theme	composite	1379:1387	arg1	hydrogels					1389:1397	loaded composite hydrogels	1372:1397	loaded composite hydrogels	1372:1397	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	5	3	theme	DFO	697:699	arg1	NPs					709:711	Both free DFO and DFO NPs	687:711	Both free DFO and DFO NPs	687:711	Both free DFO and DFO NPs were entrapped into the prepared CS-HA composite hydrogel.
29055865	3	4	theme	encapsulation	555:567	arg1	efficiency					569:578	the encapsulation efficiency	551:578	the encapsulation efficiency	551:578	The DLS and SEM analysis showed an average particle size of 220±71nm with spherical morphology and the encapsulation efficiency was found to be 30±5%.
29055865	3	4	theme	encapsulation	555:567	arg1	%					600:600	30±5%	596:600	30±5%	596:600	The DLS and SEM analysis showed an average particle size of 220±71nm with spherical morphology and the encapsulation efficiency was found to be 30±5%.
29055865	5	5	theme	DFO	705:707	arg1	NPs					709:711	Both free DFO and DFO NPs	687:711	Both free DFO and DFO NPs	687:711	Both free DFO and DFO NPs were entrapped into the prepared CS-HA composite hydrogel.
29055865	13	6	theme	NPs	1613:1615	arg1	hydrogel					1634:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	Thus, the developed DFO NPs loaded composite hydrogel could potentially be used for therapeutic angiogenesis.
29055865	10	7	theme	control	1255:1261	arg1	hydrogels					1263:1271	control hydrogels	1255:1271	control hydrogels without DFO and DFO NPs	1255:1295	The DFO and DFO NPs loaded composite hydrogels revealed effective tube formation in comparison with control hydrogels without DFO and DFO NPs.
29055865	13	8	theme	composite	1624:1632	arg1	hydrogel					1634:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	Thus, the developed DFO NPs loaded composite hydrogel could potentially be used for therapeutic angiogenesis.
29055865	8	9	theme	10days	1028:1033	arg1	period					1018:1023	a period	1016:1023	a period of 10days	1016:1033	In vitro DFO release from the prepared composite hydrogels showed controlled release over a period of 10days.
29055865	11	10	theme	DFO	1352:1354	arg1	NPs					1356:1358	DFO NPs	1352:1358	DFO NPs (0.025%w/w)	1352:1370	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	11	10	theme	DFO	1352:1354	arg1	%					1366:1366	0.025%w/w	1361:1369	0.025%w/w	1361:1369	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	5	11	theme	prepared	737:744	arg1	hydrogel					762:769	the prepared CS-HA composite hydrogel	733:769	the prepared CS-HA composite hydrogel	733:769	Both free DFO and DFO NPs were entrapped into the prepared CS-HA composite hydrogel.
29055865	13	12	theme	developed	1599:1607	arg1	hydrogel					1634:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	Thus, the developed DFO NPs loaded composite hydrogel could potentially be used for therapeutic angiogenesis.
29055865	7	13	theme	NPs	848:850	arg1	Addition					832:839	Addition	832:839	Addition of DFO NPs	832:850	Addition of DFO NPs did not affect the injectablility and flowability of developed hydrogels.
29055865	5	14	theme	composite	752:760	arg1	hydrogel					762:769	the prepared CS-HA composite hydrogel	733:769	the prepared CS-HA composite hydrogel	733:769	Both free DFO and DFO NPs were entrapped into the prepared CS-HA composite hydrogel.
29055865	1	15	theme	injectable	138:147	arg1	hydrogel					188:195	an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel	135:195	an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel	135:195	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	1	16	theme	acid	169:172	arg1	hydrogel					188:195	an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel	135:195	an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel	135:195	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	9	17	theme	excellent	1069:1077	arg1	cyto-compatability					1079:1096	excellent cyto-compatability	1069:1096	excellent cyto-compatability	1069:1096	Both the hydrogel systems showed excellent cyto-compatability and good cell proliferation for rASCs as well as HUVECs.
29055865	1	18	theme	CS-HA	175:179	arg1	hydrogel					188:195	an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel	135:195	an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel	135:195	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	11	19	theme	angiogenic	1310:1319	arg1	evaluation					1321:1330	The in vivo angiogenic evaluation	1298:1330	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels	1298:1397	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	1	20	theme	based	182:186	arg1	hydrogel					188:195	an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel	135:195	an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel	135:195	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	11	21	theme	in	1302:1303	arg1	evaluation					1321:1330	The in vivo angiogenic evaluation	1298:1330	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels	1298:1397	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	9	22	theme	cell	1107:1110	arg1	proliferation					1112:1124	good cell proliferation	1102:1124	good cell proliferation	1102:1124	Both the hydrogel systems showed excellent cyto-compatability and good cell proliferation for rASCs as well as HUVECs.
29055865	11	23	theme	free	1339:1342	arg1	DFO					1344:1346	the free DFO	1335:1346	the free DFO	1335:1346	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	13	24	used	used	1664:1667	arg2	hydrogel					1634:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	Thus, the developed DFO NPs loaded composite hydrogel could potentially be used for therapeutic angiogenesis.
29055865	11	25	dep	in	1302:1303	arg1	vivo					1305:1308	vivo	1305:1308	vivo	1305:1308	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	10	26	with	comparison	1239:1248	arg1	hydrogels					1263:1271	control hydrogels	1255:1271	control hydrogels without DFO and DFO NPs	1255:1295	The DFO and DFO NPs loaded composite hydrogels revealed effective tube formation in comparison with control hydrogels without DFO and DFO NPs.
29055865	11	27	theme	hydrogel	1439:1446	arg1	subcutaneously					1448:1461	the developed hydrogel subcutaneously	1425:1461	the developed hydrogel subcutaneously	1425:1461	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	2	28	theme	physicochemical	424:438	arg1	properties					440:449	their physicochemical properties	418:449	their physicochemical properties	418:449	DFO-NPs were prepared by double emulsion solvent diffusion technique and characterized for their physicochemical properties.
29055865	0	29	theme	Injectable	0:9	arg1	deferoxamine					11:22	Injectable deferoxamine	0:22	Injectable deferoxamine	0:22	Injectable deferoxamine nanoparticles loaded chitosan-hyaluronic acid coacervate hydrogel for therapeutic angiogenesis.
29055865	10	30	theme	DFO	1167:1169	arg1	NPs					1171:1173	The DFO and DFO NPs	1155:1173	The DFO and DFO NPs loaded composite hydrogels	1155:1200	The DFO and DFO NPs loaded composite hydrogels revealed effective tube formation in comparison with control hydrogels without DFO and DFO NPs.
29055865	10	31	theme	DFO	1159:1161	arg1	NPs					1171:1173	The DFO and DFO NPs	1155:1173	The DFO and DFO NPs loaded composite hydrogels	1155:1200	The DFO and DFO NPs loaded composite hydrogels revealed effective tube formation in comparison with control hydrogels without DFO and DFO NPs.
29055865	12	32	theme	composite	1507:1515	arg1	hydrogel					1517:1524	composite hydrogel	1507:1524	composite hydrogel	1507:1524	The DFO NPs loaded composite hydrogel had enhanced neovascularization when compared to control gels.
29055865	13	33	theme	therapeutic	1673:1683	arg1	angiogenesis					1685:1696	therapeutic angiogenesis	1673:1696	therapeutic angiogenesis	1673:1696	Thus, the developed DFO NPs loaded composite hydrogel could potentially be used for therapeutic angiogenesis.
29055865	3	34	theme	particle	495:502	arg1	size					504:507	an average particle size	484:507	an average particle size of 220±71nm	484:519	The DLS and SEM analysis showed an average particle size of 220±71nm with spherical morphology and the encapsulation efficiency was found to be 30±5%.
29055865	0	35	theme	chitosan-hyaluronic	45:63	arg1	hydrogel					81:88	loaded chitosan-hyaluronic acid coacervate hydrogel	38:88	loaded chitosan-hyaluronic acid coacervate hydrogel for therapeutic angiogenesis	38:117	Injectable deferoxamine nanoparticles loaded chitosan-hyaluronic acid coacervate hydrogel for therapeutic angiogenesis.
29055865	8	36	from	hydrogels	975:983	arg1	release					939:945	In vitro DFO release	926:945	In vitro DFO release from the prepared composite hydrogels	926:983	In vitro DFO release from the prepared composite hydrogels showed controlled release over a period of 10days.
29055865	7	37	dep	injectablility	871:884	arg1	the					867:869	the	867:869	the	867:869	Addition of DFO NPs did not affect the injectablility and flowability of developed hydrogels.
29055865	2	38	theme	diffusion	376:384	arg1	technique					386:394	double emulsion solvent diffusion technique	352:394	double emulsion solvent diffusion technique	352:394	DFO-NPs were prepared by double emulsion solvent diffusion technique and characterized for their physicochemical properties.
29055865	0	39	theme	coacervate	70:79	arg1	hydrogel					81:88	loaded chitosan-hyaluronic acid coacervate hydrogel	38:88	loaded chitosan-hyaluronic acid coacervate hydrogel for therapeutic angiogenesis	38:117	Injectable deferoxamine nanoparticles loaded chitosan-hyaluronic acid coacervate hydrogel for therapeutic angiogenesis.
29055865	8	40	theme	composite	965:973	arg1	hydrogels					975:983	the prepared composite hydrogels	952:983	the prepared composite hydrogels	952:983	In vitro DFO release from the prepared composite hydrogels showed controlled release over a period of 10days.
29055865	2	41	theme	emulsion	359:366	arg1	technique					386:394	double emulsion solvent diffusion technique	352:394	double emulsion solvent diffusion technique	352:394	DFO-NPs were prepared by double emulsion solvent diffusion technique and characterized for their physicochemical properties.
29055865	1	42	theme	loaded	262:267	arg1	nanoparticles					274:286	loaded PLGA nanoparticles	262:286	deferoxamine loaded PLGA nanoparticles (DFO NPs)	249:296	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	1	42	theme	loaded	262:267	arg1	NPs					293:295	DFO NPs	289:295	DFO NPs	289:295	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	4	43	theme	CS-HA	646:650	arg1	hydrogel					664:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel was prepared.
29055865	10	44	theme	effective	1211:1219	arg1	formation					1226:1234	effective tube formation	1211:1234	effective tube formation	1211:1234	The DFO and DFO NPs loaded composite hydrogels revealed effective tube formation in comparison with control hydrogels without DFO and DFO NPs.
29055865	4	45	theme	coacervate	653:662	arg1	hydrogel					664:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel was prepared.
29055865	11	46	theme	loaded	1372:1377	arg1	hydrogels					1389:1397	loaded composite hydrogels	1372:1397	loaded composite hydrogels	1372:1397	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	5	47	theme	free	692:695	arg1	NPs					709:711	Both free DFO and DFO NPs	687:711	Both free DFO and DFO NPs	687:711	Both free DFO and DFO NPs were entrapped into the prepared CS-HA composite hydrogel.
29055865	1	48	theme	DFO	289:291	arg1	nanoparticles					274:286	loaded PLGA nanoparticles	262:286	deferoxamine loaded PLGA nanoparticles (DFO NPs)	249:296	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	1	48	theme	DFO	289:291	arg1	NPs					293:295	DFO NPs	289:295	DFO NPs	289:295	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	4	49	theme	mimicking	610:618	arg1	hydrogel					664:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel was prepared.
29055865	13	50	theme	DFO	1609:1611	arg1	hydrogel					1634:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	Thus, the developed DFO NPs loaded composite hydrogel could potentially be used for therapeutic angiogenesis.
29055865	7	51	theme	developed	905:913	arg1	hydrogels					915:923	developed hydrogels	905:923	developed hydrogels	905:923	Addition of DFO NPs did not affect the injectablility and flowability of developed hydrogels.
29055865	4	52	theme	acid	640:643	arg1	hydrogel					664:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel was prepared.
29055865	8	53	dep	In	926:927	arg1	vitro					929:933	vitro	929:933	vitro	929:933	In vitro DFO release from the prepared composite hydrogels showed controlled release over a period of 10days.
29055865	13	54	theme	loaded	1617:1622	arg1	hydrogel					1634:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	the developed DFO NPs loaded composite hydrogel	1595:1641	Thus, the developed DFO NPs loaded composite hydrogel could potentially be used for therapeutic angiogenesis.
29055865	11	55	theme	NPs	1356:1358	arg1	evaluation					1321:1330	The in vivo angiogenic evaluation	1298:1330	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels	1298:1397	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	1	56	theme	pro-angiogenic	224:237	arg1	molecule					239:246	pro-angiogenic molecule	224:246	pro-angiogenic molecule	224:246	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	1	56	theme	pro-angiogenic	224:237	arg1	deferoxamine					249:260	deferoxamine	249:260	deferoxamine loaded PLGA nanoparticles (DFO NPs)	249:296	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	11	57	theme	w/w	1367:1369	arg1	NPs					1356:1358	DFO NPs	1352:1358	DFO NPs (0.025%w/w)	1352:1370	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	11	57	theme	w/w	1367:1369	arg1	%					1366:1366	0.025%w/w	1361:1369	0.025%w/w	1361:1369	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	5	58	theme	CS-HA	746:750	arg1	hydrogel					762:769	the prepared CS-HA composite hydrogel	733:769	the prepared CS-HA composite hydrogel	733:769	Both free DFO and DFO NPs were entrapped into the prepared CS-HA composite hydrogel.
29055865	9	59	theme	hydrogel	1045:1052	arg1	systems					1054:1060	Both the hydrogel systems	1036:1060	Both the hydrogel systems	1036:1060	Both the hydrogel systems showed excellent cyto-compatability and good cell proliferation for rASCs as well as HUVECs.
29055865	1	60	theme	chitosan-hyaluronic	149:167	arg1	hydrogel					188:195	an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel	135:195	an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel	135:195	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	12	61	theme	control	1575:1581	arg1	gels					1583:1586	control gels	1575:1586	control gels	1575:1586	The DFO NPs loaded composite hydrogel had enhanced neovascularization when compared to control gels.
29055865	0	62	theme	therapeutic	94:104	arg1	angiogenesis					106:117	therapeutic angiogenesis	94:117	therapeutic angiogenesis	94:117	Injectable deferoxamine nanoparticles loaded chitosan-hyaluronic acid coacervate hydrogel for therapeutic angiogenesis.
29055865	9	63	theme	good	1102:1105	arg1	proliferation					1112:1124	good cell proliferation	1102:1124	good cell proliferation	1102:1124	Both the hydrogel systems showed excellent cyto-compatability and good cell proliferation for rASCs as well as HUVECs.
29055865	3	64	theme	spherical	526:534	arg1	morphology					536:545	spherical morphology	526:545	spherical morphology	526:545	The DLS and SEM analysis showed an average particle size of 220±71nm with spherical morphology and the encapsulation efficiency was found to be 30±5%.
29055865	11	65	theme	DFO	1344:1346	arg1	evaluation					1321:1330	The in vivo angiogenic evaluation	1298:1330	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels	1298:1397	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	1	66	theme	PLGA	269:272	arg1	nanoparticles					274:286	loaded PLGA nanoparticles	262:286	deferoxamine loaded PLGA nanoparticles (DFO NPs)	249:296	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	1	66	theme	PLGA	269:272	arg1	NPs					293:295	DFO NPs	289:295	DFO NPs	289:295	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	11	67	theme	developed	1429:1437	arg1	subcutaneously					1448:1461	the developed hydrogel subcutaneously	1425:1461	the developed hydrogel subcutaneously	1425:1461	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	3	68	theme	average	487:493	arg1	size					504:507	an average particle size	484:507	an average particle size of 220±71nm	484:519	The DLS and SEM analysis showed an average particle size of 220±71nm with spherical morphology and the encapsulation efficiency was found to be 30±5%.
29055865	0	69	theme	loaded	38:43	arg1	hydrogel					81:88	loaded chitosan-hyaluronic acid coacervate hydrogel	38:88	loaded chitosan-hyaluronic acid coacervate hydrogel for therapeutic angiogenesis	38:117	Injectable deferoxamine nanoparticles loaded chitosan-hyaluronic acid coacervate hydrogel for therapeutic angiogenesis.
29055865	7	70	theme	DFO	844:846	arg1	NPs					848:850	DFO NPs	844:850	DFO NPs	844:850	Addition of DFO NPs did not affect the injectablility and flowability of developed hydrogels.
29055865	10	71	dep	DFO	1281:1283	arg1	NPs					1293:1295	NPs	1293:1295	NPs	1293:1295	The DFO and DFO NPs loaded composite hydrogels revealed effective tube formation in comparison with control hydrogels without DFO and DFO NPs.
29055865	8	72	theme	In	926:927	arg1	release					939:945	In vitro DFO release	926:945	In vitro DFO release from the prepared composite hydrogels	926:983	In vitro DFO release from the prepared composite hydrogels showed controlled release over a period of 10days.
29055865	4	73	theme	ECM	606:608	arg1	hydrogel					664:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel	603:671	An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel was prepared.
29055865	3	74	theme	220±71nm	512:519	arg1	size					504:507	an average particle size	484:507	an average particle size of 220±71nm	484:519	The DLS and SEM analysis showed an average particle size of 220±71nm with spherical morphology and the encapsulation efficiency was found to be 30±5%.
29055865	0	75	theme	acid	65:68	arg1	hydrogel					81:88	loaded chitosan-hyaluronic acid coacervate hydrogel	38:88	loaded chitosan-hyaluronic acid coacervate hydrogel for therapeutic angiogenesis	38:117	Injectable deferoxamine nanoparticles loaded chitosan-hyaluronic acid coacervate hydrogel for therapeutic angiogenesis.
29055865	8	76	theme	DFO	935:937	arg1	release					939:945	In vitro DFO release	926:945	In vitro DFO release from the prepared composite hydrogels	926:983	In vitro DFO release from the prepared composite hydrogels showed controlled release over a period of 10days.
29055865	3	77	theme	SEM	464:466	arg1	analysis					468:475	SEM analysis	464:475	SEM analysis	464:475	The DLS and SEM analysis showed an average particle size of 220±71nm with spherical morphology and the encapsulation efficiency was found to be 30±5%.
29055865	10	78	theme	composite	1182:1190	arg1	hydrogels					1192:1200	composite hydrogels	1182:1200	composite hydrogels	1182:1200	The DFO and DFO NPs loaded composite hydrogels revealed effective tube formation in comparison with control hydrogels without DFO and DFO NPs.
29055865	11	79	dep	DFO	1344:1346	arg1	hydrogels					1389:1397	loaded composite hydrogels	1372:1397	loaded composite hydrogels	1372:1397	The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks.
29055865	2	80	theme	solvent	368:374	arg1	technique					386:394	double emulsion solvent diffusion technique	352:394	double emulsion solvent diffusion technique	352:394	DFO-NPs were prepared by double emulsion solvent diffusion technique and characterized for their physicochemical properties.
29055865	8	81	theme	prepared	956:963	arg1	hydrogels					975:983	the prepared composite hydrogels	952:983	the prepared composite hydrogels	952:983	In vitro DFO release from the prepared composite hydrogels showed controlled release over a period of 10days.
29055865	1	82	dep	deferoxamine	249:260	arg1	nanoparticles					274:286	loaded PLGA nanoparticles	262:286	deferoxamine loaded PLGA nanoparticles (DFO NPs)	249:296	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	1	82	dep	deferoxamine	249:260	arg1	NPs					293:295	DFO NPs	289:295	DFO NPs	289:295	In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis.
29055865	2	83	theme	double	352:357	arg1	technique					386:394	double emulsion solvent diffusion technique	352:394	double emulsion solvent diffusion technique	352:394	DFO-NPs were prepared by double emulsion solvent diffusion technique and characterized for their physicochemical properties.
29055865	10	84	theme	tube	1221:1224	arg1	formation					1226:1234	effective tube formation	1211:1234	effective tube formation	1211:1234	The DFO and DFO NPs loaded composite hydrogels revealed effective tube formation in comparison with control hydrogels without DFO and DFO NPs.
29055865	12	85	theme	DFO	1492:1494	arg1	NPs					1496:1498	The DFO NPs	1488:1498	The DFO NPs loaded composite hydrogel	1488:1524	The DFO NPs loaded composite hydrogel had enhanced neovascularization when compared to control gels.
29055865	7	86	theme	hydrogels	915:923	arg1	injectablility					871:884	injectablility	871:884	injectablility	871:884	Addition of DFO NPs did not affect the injectablility and flowability of developed hydrogels.
29055865	7	86	theme	hydrogels	915:923	arg1	flowability					890:900	flowability	890:900	flowability	890:900	Addition of DFO NPs did not affect the injectablility and flowability of developed hydrogels.
24751258	0	0	theme	AgNPs-alginate	85:98	arg1	composite					100:108	AgNPs-alginate composite	85:108	AgNPs-alginate composite	85:108	Surface modification of cotton fabrics for antibacterial application by coating with AgNPs-alginate composite.
24751258	3	1	theme	SEM	523:525	arg1	images					527:532	SEM images	523:532	SEM images	523:532	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	2	2	theme	alginate	382:389	arg1	solution					391:398	alkali hydrolyzed alginate solution	364:398	alkali hydrolyzed alginate solution which acts as both reducing and capping agent	364:444	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	3	3	theme	physical	585:592	arg1	deposition					594:603	the successful physical deposition	570:603	the successful physical deposition of AgNPs-alginate composite on the fabric	570:645	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	2	4	theme	synthetic	311:319	arg1	route					321:325	a simple one-step rapid synthetic route	287:325	a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent	287:444	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	3	5	theme	release	503:509	arg1	percent					511:517	silver release percent	496:517	silver release percent	496:517	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	2	6	theme	rapid	305:309	arg1	route					321:325	a simple one-step rapid synthetic route	287:325	a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent	287:444	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	3	7	theme	color	461:465	arg1	coordinates					467:477	color coordinates	461:477	color coordinates	461:477	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	6	8	theme	antibacterial	945:957	arg1	activity					959:966	an efficient antibacterial activity	932:966	an efficient antibacterial activity	932:966	However, an efficient antibacterial activity still remained on the fabrics.
24751258	2	9	theme	one-step	296:303	arg1	route					321:325	a simple one-step rapid synthetic route	287:325	a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent	287:444	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	4	10	theme	antibacterial	694:706	arg1	activity					708:715	an excellent antibacterial activity	681:715	an excellent antibacterial activity against the tested bacteria, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa	681:811	The treated fabrics demonstrated an excellent antibacterial activity against the tested bacteria, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24751258	6	11	theme	efficient	935:943	arg1	activity					959:966	an efficient antibacterial activity	932:966	an efficient antibacterial activity	932:966	However, an efficient antibacterial activity still remained on the fabrics.
24751258	2	12	theme	silver	343:348	arg1	nitrate					350:356	silver nitrate	343:356	silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent	343:444	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	0	13	theme	Surface	0:6	arg1	modification					8:19	Surface modification	0:19	Surface modification of cotton fabrics for antibacterial application by coating with AgNPs-alginate composite.	0:109	Surface modification of cotton fabrics for antibacterial application by coating with AgNPs-alginate composite.
24751258	5	14	from	decrease	823:830	arg1	feature					853:859	the antibacterial feature	835:859	the antibacterial feature of the cotton fabrics	835:881	A slight decrease in the antibacterial feature of the cotton fabrics was observed after successive washings.
24751258	0	15	theme	cotton	24:29	arg1	fabrics					31:37	cotton fabrics	24:37	cotton fabrics for antibacterial application	24:67	Surface modification of cotton fabrics for antibacterial application by coating with AgNPs-alginate composite.
24751258	5	16	theme	cotton	868:873	arg1	fabrics					875:881	the cotton fabrics	864:881	the cotton fabrics	864:881	A slight decrease in the antibacterial feature of the cotton fabrics was observed after successive washings.
24751258	3	17	theme	successful	574:583	arg1	deposition					594:603	the successful physical deposition	570:603	the successful physical deposition of AgNPs-alginate composite on the fabric	570:645	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	3	18	theme	fabric	545:550	arg1	samples					552:558	treated fabric samples	537:558	treated fabric samples	537:558	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	2	19	theme	antimicrobial	201:213	arg1	activities					215:224	antimicrobial activities	201:224	antimicrobial activities	201:224	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	1	20	theme	recent	114:119	arg1	years					121:125	recent years	114:125	recent years	114:125	In recent years nano-sized particles have been focused on bacteriostasis.
24751258	5	21	theme	fabrics	875:881	arg1	feature					853:859	the antibacterial feature	835:859	the antibacterial feature of the cotton fabrics	835:881	A slight decrease in the antibacterial feature of the cotton fabrics was observed after successive washings.
24751258	2	22	theme	alkali	364:369	arg1	solution					391:398	alkali hydrolyzed alginate solution	364:398	alkali hydrolyzed alginate solution which acts as both reducing and capping agent	364:444	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	5	23	theme	successive	902:911	arg1	washings					913:920	successive washings	902:920	successive washings	902:920	A slight decrease in the antibacterial feature of the cotton fabrics was observed after successive washings.
24751258	4	24	theme	excellent	684:692	arg1	activity					708:715	an excellent antibacterial activity	681:715	an excellent antibacterial activity against the tested bacteria, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa	681:811	The treated fabrics demonstrated an excellent antibacterial activity against the tested bacteria, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24751258	0	25	theme	fabrics	31:37	arg1	modification					8:19	Surface modification	0:19	Surface modification of cotton fabrics for antibacterial application by coating with AgNPs-alginate composite.	0:109	Surface modification of cotton fabrics for antibacterial application by coating with AgNPs-alginate composite.
24751258	3	26	theme	FTIR	447:450	arg1	spectra					452:458	FTIR spectra	447:458	FTIR spectra	447:458	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	2	27	theme	cotton	266:271	arg1	fabric					273:278	cotton fabric	266:278	cotton fabric	266:278	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	2	28	theme	capping	432:438	arg1	agent					440:444	both reducing and capping agent	414:444	agent	440:444	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	3	29	theme	AgNPs-alginate	608:621	arg1	composite					623:631	AgNPs-alginate composite	608:631	AgNPs-alginate composite	608:631	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	2	30	theme	hydrolyzed	371:380	arg1	solution					391:398	alkali hydrolyzed alginate solution	364:398	alkali hydrolyzed alginate solution which acts as both reducing and capping agent	364:444	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	0	31	theme	antibacterial	43:55	arg1	application					57:67	antibacterial application	43:67	antibacterial application	43:67	Surface modification of cotton fabrics for antibacterial application by coating with AgNPs-alginate composite.
24751258	2	32	theme	AgNPs-alginate	238:251	arg1	composite					253:261	AgNPs-alginate composite	238:261	AgNPs-alginate composite	238:261	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	3	33	theme	composite	623:631	arg1	deposition					594:603	the successful physical deposition	570:603	the successful physical deposition of AgNPs-alginate composite on the fabric	570:645	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	1	34	theme	nano-sized	127:136	arg1	particles					138:146	nano-sized particles	127:146	nano-sized particles	127:146	In recent years nano-sized particles have been focused on bacteriostasis.
24751258	0	35	mod	modification	8:19	arg3	Surface					0:6	Surface modification	0:19	Surface modification of cotton fabrics for antibacterial application by coating with AgNPs-alginate composite.	0:109	Surface modification of cotton fabrics for antibacterial application by coating with AgNPs-alginate composite.
24751258	0	35	mod	modification	8:19	arg1	fabrics					31:37	cotton fabrics	24:37	cotton fabrics for antibacterial application	24:67	Surface modification of cotton fabrics for antibacterial application by coating with AgNPs-alginate composite.
24751258	2	36	theme	simple	289:294	arg1	route					321:325	a simple one-step rapid synthetic route	287:325	a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent	287:444	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	4	37	theme	tested	729:734	arg1	aeruginosa					802:811	Pseudomonas aeruginosa	790:811	Pseudomonas aeruginosa	790:811	The treated fabrics demonstrated an excellent antibacterial activity against the tested bacteria, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24751258	4	37	theme	tested	729:734	arg1	bacteria					736:743	the tested bacteria	725:743	the tested bacteria	725:743	The treated fabrics demonstrated an excellent antibacterial activity against the tested bacteria, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24751258	4	37	theme	tested	729:734	arg1	coli					758:761	Escherichia coli	746:761	Escherichia coli	746:761	The treated fabrics demonstrated an excellent antibacterial activity against the tested bacteria, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24751258	4	37	theme	tested	729:734	arg1	aureus					779:784	Staphylococcus aureus	764:784	Staphylococcus aureus	764:784	The treated fabrics demonstrated an excellent antibacterial activity against the tested bacteria, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24751258	3	38	theme	silver	496:501	arg1	percent					511:517	silver release percent	496:517	silver release percent	496:517	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	3	39	theme	samples	552:558	arg1	percent					511:517	silver release percent	496:517	silver release percent	496:517	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	3	39	theme	samples	552:558	arg1	images					527:532	SEM images	523:532	SEM images	523:532	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	3	39	theme	samples	552:558	arg1	spectra					452:458	FTIR spectra	447:458	FTIR spectra	447:458	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	3	39	theme	samples	552:558	arg1	coordinates					467:477	color coordinates	461:477	color coordinates	461:477	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	3	39	theme	samples	552:558	arg1	content					487:493	silver content	480:493	silver content	480:493	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	2	40	theme	nitrate	350:356	arg1	reduction					330:338	reduction	330:338	reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent	330:444	We investigated antimicrobial activities by applying AgNPs-alginate composite on cotton fabric, using a simple one-step rapid synthetic route by reduction of silver nitrate using alkali hydrolyzed alginate solution which acts as both reducing and capping agent.
24751258	3	41	from	deposition	594:603	arg1	fabric					640:645	the fabric	636:645	the fabric	636:645	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	3	42	theme	silver	480:485	arg1	content					487:493	silver content	480:493	silver content	480:493	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
24751258	4	43	theme	treated	652:658	arg1	fabrics					660:666	The treated fabrics	648:666	The treated fabrics	648:666	The treated fabrics demonstrated an excellent antibacterial activity against the tested bacteria, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
24751258	5	44	theme	slight	816:821	arg1	decrease					823:830	A slight decrease	814:830	A slight decrease in the antibacterial feature of the cotton fabrics	814:881	A slight decrease in the antibacterial feature of the cotton fabrics was observed after successive washings.
24751258	5	45	theme	antibacterial	839:851	arg1	feature					853:859	the antibacterial feature	835:859	the antibacterial feature of the cotton fabrics	835:881	A slight decrease in the antibacterial feature of the cotton fabrics was observed after successive washings.
24751258	3	46	theme	treated	537:543	arg1	samples					552:558	treated fabric samples	537:558	treated fabric samples	537:558	FTIR spectra, color coordinates, silver content, silver release percent and SEM images of treated fabric samples confirmed the successful physical deposition of AgNPs-alginate composite on the fabric.
26202543	0	0	theme	Model	80:84	arg1	Network					107:113	a Model Polydimethylsiloxane Network	78:113	a Model Polydimethylsiloxane Network	78:113	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.
26202543	2	1	theme	methyltrimethoxysilane	377:398	arg1	presence					365:372	the presence	361:372	the presence of methyltrimethoxysilane used as a model alkoxysilane	361:427	NFC suspensions were freeze-dried under controlled conditions in the presence of methyltrimethoxysilane used as a model alkoxysilane.
26202543	1	2	theme	sol-gel	258:264	arg1	route					266:270	an environmentally friendly sol-gel route	230:270	an environmentally friendly sol-gel route based on alkoxysilanes	230:293	A comparative approach for the surface silylation of nanofibrillated cellulose (NFC) in water is proposed through an environmentally friendly sol-gel route based on alkoxysilanes.
26202543	0	3	from	Silylation	11:20	arg1	Water					54:58	Water	54:58	Water	54:58	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.
26202543	1	4	theme	nanofibrillated	169:183	arg1	NFC					196:198	NFC	196:198	NFC	196:198	A comparative approach for the surface silylation of nanofibrillated cellulose (NFC) in water is proposed through an environmentally friendly sol-gel route based on alkoxysilanes.
26202543	1	4	theme	nanofibrillated	169:183	arg1	cellulose					185:193	nanofibrillated cellulose	169:193	nanofibrillated cellulose (NFC)	169:199	A comparative approach for the surface silylation of nanofibrillated cellulose (NFC) in water is proposed through an environmentally friendly sol-gel route based on alkoxysilanes.
26202543	0	5	theme	Network	107:113	arg1	Reinforcement					61:73	Reinforcement	61:73	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.	0:114	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.
26202543	4	6	theme	cellulosic	690:699	arg1	fibrils					701:707	cellulosic fibrils	690:707	cellulosic fibrils coated by a polysiloxane layer bonded firmly to the cellulosic substrate	690:780	Protocol 1 led to a network of nanofibrils in which polysiloxane particles were dispersed, and protocol 2 produced a scaffold of cellulosic fibrils coated by a polysiloxane layer bonded firmly to the cellulosic substrate.
26202543	0	7	theme	Polydimethylsiloxane	86:105	arg1	Network					107:113	a Model Polydimethylsiloxane Network	78:113	a Model Polydimethylsiloxane Network	78:113	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.
26202543	6	8	theme	fibrils	941:947	arg1	fibrils					941:947	fibrils	941:947	fibrils treated by protocol 2	941:969	Moreover, if 1 wt % of fibrils treated by protocol 2 were incorporated into a model polydimethylsiloxane network, substantial improvements of the static and dynamic mechanical properties of the composite were noted.
26202543	6	8	theme	fibrils	941:947	arg1	%					936:936	1 wt %	931:936	1 wt % of fibrils treated by protocol 2	931:969	Moreover, if 1 wt % of fibrils treated by protocol 2 were incorporated into a model polydimethylsiloxane network, substantial improvements of the static and dynamic mechanical properties of the composite were noted.
26202543	5	9	with	material	845:852	arg1	properties					871:880	hydrophobic properties	859:880	hydrophobic properties	859:880	Different from protocol 1, protocol 2 imparted the cellulosic material with hydrophobic properties and improved its thermal stability.
26202543	2	10	theme	NFC	296:298	arg1	suspensions					300:310	NFC suspensions	296:310	NFC suspensions	296:310	NFC suspensions were freeze-dried under controlled conditions in the presence of methyltrimethoxysilane used as a model alkoxysilane.
26202543	4	11	theme	polysiloxane	613:624	arg1	particles					626:634	polysiloxane particles	613:634	polysiloxane particles	613:634	Protocol 1 led to a network of nanofibrils in which polysiloxane particles were dispersed, and protocol 2 produced a scaffold of cellulosic fibrils coated by a polysiloxane layer bonded firmly to the cellulosic substrate.
26202543	3	12	theme	post-treatment	503:516	arg1	procedures					518:527	post-treatment procedures	503:527	post-treatment procedures	503:527	Two different protocols that involve different pH values (0.4 and 4) and post-treatment procedures were investigated and compared.
26202543	1	13	from	silylation	155:164	arg1	water					204:208	water	204:208	water	204:208	A comparative approach for the surface silylation of nanofibrillated cellulose (NFC) in water is proposed through an environmentally friendly sol-gel route based on alkoxysilanes.
26202543	1	14	theme	cellulose	185:193	arg1	silylation					155:164	the surface silylation	143:164	the surface silylation of nanofibrillated cellulose (NFC) in water	143:208	A comparative approach for the surface silylation of nanofibrillated cellulose (NFC) in water is proposed through an environmentally friendly sol-gel route based on alkoxysilanes.
26202543	6	15	theme	static	1064:1069	arg1	properties					1094:1103	the static and dynamic mechanical properties	1060:1103	the static and dynamic mechanical properties of the composite	1060:1120	Moreover, if 1 wt % of fibrils treated by protocol 2 were incorporated into a model polydimethylsiloxane network, substantial improvements of the static and dynamic mechanical properties of the composite were noted.
26202543	5	16	theme	hydrophobic	859:869	arg1	properties					871:880	hydrophobic properties	859:880	hydrophobic properties	859:880	Different from protocol 1, protocol 2 imparted the cellulosic material with hydrophobic properties and improved its thermal stability.
26202543	2	17	theme	controlled	336:345	arg1	conditions					347:356	controlled conditions	336:356	controlled conditions	336:356	NFC suspensions were freeze-dried under controlled conditions in the presence of methyltrimethoxysilane used as a model alkoxysilane.
26202543	2	18	theme	model	410:414	arg1	alkoxysilane					416:427	a model alkoxysilane	408:427	a model alkoxysilane	408:427	NFC suspensions were freeze-dried under controlled conditions in the presence of methyltrimethoxysilane used as a model alkoxysilane.
26202543	0	19	theme	Controlled	0:9	arg1	Silylation					11:20	Controlled Silylation	0:20	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.	0:114	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.
26202543	6	20	theme	substantial	1032:1042	arg1	improvements					1044:1055	substantial improvements	1032:1055	substantial improvements of the static and dynamic mechanical properties of the composite	1032:1120	Moreover, if 1 wt % of fibrils treated by protocol 2 were incorporated into a model polydimethylsiloxane network, substantial improvements of the static and dynamic mechanical properties of the composite were noted.
26202543	4	21	theme	cellulosic	761:770	arg1	substrate					772:780	the cellulosic substrate	757:780	the cellulosic substrate	757:780	Protocol 1 led to a network of nanofibrils in which polysiloxane particles were dispersed, and protocol 2 produced a scaffold of cellulosic fibrils coated by a polysiloxane layer bonded firmly to the cellulosic substrate.
26202543	3	22	theme	different	467:475	arg1	values					480:485	different pH values	467:485	different pH values (0.4 and 4)	467:497	Two different protocols that involve different pH values (0.4 and 4) and post-treatment procedures were investigated and compared.
26202543	0	23	theme	Nanofibrillated	25:39	arg1	Cellulose					41:49	Nanofibrillated Cellulose	25:49	Nanofibrillated Cellulose	25:49	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.
26202543	4	24	theme	nanofibrils	592:602	arg1	network					581:587	a network	579:587	a network of nanofibrils in which polysiloxane particles were dispersed, and protocol 2 produced a scaffold of cellulosic fibrils coated by a polysiloxane layer bonded firmly to the cellulosic substrate	579:780	Protocol 1 led to a network of nanofibrils in which polysiloxane particles were dispersed, and protocol 2 produced a scaffold of cellulosic fibrils coated by a polysiloxane layer bonded firmly to the cellulosic substrate.
26202543	4	25	theme	polysiloxane	721:732	arg1	layer					734:738	a polysiloxane layer	719:738	a polysiloxane layer bonded firmly to the cellulosic substrate	719:780	Protocol 1 led to a network of nanofibrils in which polysiloxane particles were dispersed, and protocol 2 produced a scaffold of cellulosic fibrils coated by a polysiloxane layer bonded firmly to the cellulosic substrate.
26202543	6	26	theme	composite	1112:1120	arg1	properties					1094:1103	the static and dynamic mechanical properties	1060:1103	the static and dynamic mechanical properties of the composite	1060:1120	Moreover, if 1 wt % of fibrils treated by protocol 2 were incorporated into a model polydimethylsiloxane network, substantial improvements of the static and dynamic mechanical properties of the composite were noted.
26202543	6	27	theme	polydimethylsiloxane	1002:1021	arg1	network					1023:1029	a model polydimethylsiloxane network	994:1029	a model polydimethylsiloxane network	994:1029	Moreover, if 1 wt % of fibrils treated by protocol 2 were incorporated into a model polydimethylsiloxane network, substantial improvements of the static and dynamic mechanical properties of the composite were noted.
26202543	4	28	theme	fibrils	701:707	arg1	scaffold					678:685	a scaffold	676:685	a scaffold of cellulosic fibrils coated by a polysiloxane layer bonded firmly to the cellulosic substrate	676:780	Protocol 1 led to a network of nanofibrils in which polysiloxane particles were dispersed, and protocol 2 produced a scaffold of cellulosic fibrils coated by a polysiloxane layer bonded firmly to the cellulosic substrate.
26202543	5	29	theme	thermal	899:905	arg1	stability					907:915	its thermal stability	895:915	its thermal stability	895:915	Different from protocol 1, protocol 2 imparted the cellulosic material with hydrophobic properties and improved its thermal stability.
26202543	0	30	theme	Cellulose	41:49	arg1	Silylation					11:20	Controlled Silylation	0:20	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.	0:114	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.
26202543	6	31	theme	model	996:1000	arg1	network					1023:1029	a model polydimethylsiloxane network	994:1029	a model polydimethylsiloxane network	994:1029	Moreover, if 1 wt % of fibrils treated by protocol 2 were incorporated into a model polydimethylsiloxane network, substantial improvements of the static and dynamic mechanical properties of the composite were noted.
26202543	6	32	theme	dynamic	1075:1081	arg1	properties					1094:1103	the static and dynamic mechanical properties	1060:1103	the static and dynamic mechanical properties of the composite	1060:1120	Moreover, if 1 wt % of fibrils treated by protocol 2 were incorporated into a model polydimethylsiloxane network, substantial improvements of the static and dynamic mechanical properties of the composite were noted.
26202543	5	33	theme	cellulosic	834:843	arg1	material					845:852	the cellulosic material	830:852	the cellulosic material with hydrophobic properties	830:880	Different from protocol 1, protocol 2 imparted the cellulosic material with hydrophobic properties and improved its thermal stability.
26202543	3	34	theme	pH	477:478	arg1	values					480:485	different pH values	467:485	different pH values (0.4 and 4)	467:497	Two different protocols that involve different pH values (0.4 and 4) and post-treatment procedures were investigated and compared.
26202543	1	35	theme	comparative	118:128	arg1	approach					130:137	A comparative approach	116:137	A comparative approach for the surface silylation of nanofibrillated cellulose (NFC) in water	116:208	A comparative approach for the surface silylation of nanofibrillated cellulose (NFC) in water is proposed through an environmentally friendly sol-gel route based on alkoxysilanes.
26202543	3	36	theme	different	434:442	arg1	protocols					444:452	Two different protocols	430:452	Two different protocols that involve different pH values (0.4 and 4) and post-treatment procedures	430:527	Two different protocols that involve different pH values (0.4 and 4) and post-treatment procedures were investigated and compared.
26202543	3	37	dep	values	480:485	arg1	0.4					488:490	0.4	488:490	0.4	488:490	Two different protocols that involve different pH values (0.4 and 4) and post-treatment procedures were investigated and compared.
26202543	3	37	dep	values	480:485	arg1	4					496:496	4	496:496	4	496:496	Two different protocols that involve different pH values (0.4 and 4) and post-treatment procedures were investigated and compared.
26202543	6	38	theme	properties	1094:1103	arg1	improvements					1044:1055	substantial improvements	1032:1055	substantial improvements of the static and dynamic mechanical properties of the composite	1032:1120	Moreover, if 1 wt % of fibrils treated by protocol 2 were incorporated into a model polydimethylsiloxane network, substantial improvements of the static and dynamic mechanical properties of the composite were noted.
26202543	6	39	theme	mechanical	1083:1092	arg1	properties					1094:1103	the static and dynamic mechanical properties	1060:1103	the static and dynamic mechanical properties of the composite	1060:1120	Moreover, if 1 wt % of fibrils treated by protocol 2 were incorporated into a model polydimethylsiloxane network, substantial improvements of the static and dynamic mechanical properties of the composite were noted.
26202543	5	40	from	protocol 1	798:807	arg1	Different					783:791	Different	783:791	Different	783:791	Different from protocol 1, protocol 2 imparted the cellulosic material with hydrophobic properties and improved its thermal stability.
26202543	0	41	dep	Silylation	11:20	arg1	Reinforcement					61:73	Reinforcement	61:73	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.	0:114	Controlled Silylation of Nanofibrillated Cellulose in Water: Reinforcement of a Model Polydimethylsiloxane Network.
26202543	1	42	theme	surface	147:153	arg1	silylation					155:164	the surface silylation	143:164	the surface silylation of nanofibrillated cellulose (NFC) in water	143:208	A comparative approach for the surface silylation of nanofibrillated cellulose (NFC) in water is proposed through an environmentally friendly sol-gel route based on alkoxysilanes.
26202543	1	43	theme	environmentally	233:247	arg1	route					266:270	an environmentally friendly sol-gel route	230:270	an environmentally friendly sol-gel route based on alkoxysilanes	230:293	A comparative approach for the surface silylation of nanofibrillated cellulose (NFC) in water is proposed through an environmentally friendly sol-gel route based on alkoxysilanes.
26202543	1	44	theme	friendly	249:256	arg1	route					266:270	an environmentally friendly sol-gel route	230:270	an environmentally friendly sol-gel route based on alkoxysilanes	230:293	A comparative approach for the surface silylation of nanofibrillated cellulose (NFC) in water is proposed through an environmentally friendly sol-gel route based on alkoxysilanes.
28196092	7	0	dep	140	1054:1056	arg1	to					1051:1052	to	1051:1052	to	1051:1052	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	2	1	theme	asymmetric	354:363	arg1	AF4					396:398	AF4	396:398	AF4	396:398	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	2	1	theme	asymmetric	354:363	arg1	fractionation					381:393	asymmetric flow field-flow fractionation	354:393	asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection	354:514	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	6	2	theme	degradation	901:911	arg1	impurities					885:894	The main impurities	876:894	The main impurities	876:894	The main impurities were degradation products of starch.
28196092	6	2	theme	degradation	901:911	arg1	products					913:920	degradation products	901:920	degradation products of starch	901:930	The main impurities were degradation products of starch.
28196092	1	3	from	oat	162:164	arg1	β-glucan					148:155	mixed-linkage β-glucan	134:155	mixed-linkage β-glucan from oat and barley	134:175	An extraction method for mixed-linkage β-glucan from oat and barley was developed in order to minimize the effect of extraction on the β-glucan structure.
28196092	1	4	theme	β-glucan	244:251	arg1	structure					253:261	the β-glucan structure	240:261	the β-glucan structure	240:261	An extraction method for mixed-linkage β-glucan from oat and barley was developed in order to minimize the effect of extraction on the β-glucan structure.
28196092	0	5	theme	matter	101:106	arg1	quantification					69:82	quantification	69:82	quantification of proteinaceous matter	69:106	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	0	5	theme	matter	101:106	arg1	Characterization					0:15	Characterization	0:15	Characterization of cereal β-glucan extracts from oat and barley	0:63	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	0	6	from	Characterization	0:15	arg1	barley					58:63	barley	58:63	barley	58:63	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	0	6	from	Characterization	0:15	arg1	oat					50:52	oat	50:52	oat	50:52	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	2	7	theme	FL	502:503	arg1	detection					506:514	fluorescence (FL) detection	488:514	fluorescence (FL) detection	488:514	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	3	8	theme	carbohydrate	521:532	arg1	composition					534:544	The carbohydrate composition	517:544	The carbohydrate composition of the extracts	517:560	The carbohydrate composition of the extracts was analysed using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC).
28196092	7	9	from	nm	1058:1059	arg1	radii					1037:1041	root-mean-square radii	1020:1041	root-mean-square radii from 20 to 140 nm	1020:1059	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	0	10	from	oat	50:52	arg1	quantification					69:82	quantification	69:82	quantification of proteinaceous matter	69:106	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	0	10	from	oat	50:52	arg1	Characterization					0:15	Characterization	0:15	Characterization of cereal β-glucan extracts from oat and barley	0:63	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	0	10	from	oat	50:52	arg1	extracts					36:43	cereal β-glucan extracts	20:43	cereal β-glucan extracts from oat and barley	20:63	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	5	11	theme	β-glucan	832:839	arg1	%					830:830	53%	828:830	53% β-glucan for oats and barley	828:859	Purified extracts contained 65% and 53% β-glucan for oats and barley, respectively.
28196092	5	12	theme	Purified	792:799	arg1	extracts					801:808	Purified extracts	792:808	Purified extracts	792:808	Purified extracts contained 65% and 53% β-glucan for oats and barley, respectively.
28196092	2	13	theme	fluorescence	488:499	arg1	detection					506:514	fluorescence (FL) detection	488:514	fluorescence (FL) detection	488:514	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	7	14	contain	contained	946:954	arg2	weight					971:976	high molecular weight β-glucan	956:985	high molecular weight β-glucan (105-108 g/mol)	956:1001	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	7	14	contain	contained	946:954	arg2	sizes					1013:1017	large sizes	1007:1017	large sizes (root-mean-square radii from 20 to 140 nm)	1007:1060	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	7	14	contain	contained	946:954	arg1	extracts					937:944	The extracts	933:944	The extracts	933:944	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	7	14	contain	contained	946:954	arg2	g/mol					996:1000	105-108 g/mol	988:1000	105-108 g/mol	988:1000	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	8	15	theme	proteins	1161:1168	arg1	functionality					1144:1156	any suggested functionality	1130:1156	any suggested functionality of proteins regarding the health benefits of β-glucan	1130:1210	No proteins covalently bound to β-glucan were detected; therefore, any suggested functionality of proteins regarding the health benefits of β-glucan can be discounted.
28196092	2	16	theme	size	314:317	arg1	terms					295:299	terms	295:299	terms of molecular size and molar mass distributions	295:346	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	6	17	theme	main	880:883	arg1	impurities					885:894	The main impurities	876:894	The main impurities	876:894	The main impurities were degradation products of starch.
28196092	6	17	theme	main	880:883	arg1	products					913:920	degradation products	901:920	degradation products of starch	901:930	The main impurities were degradation products of starch.
28196092	7	18	dep	sizes	1013:1017	arg1	radii					1037:1041	root-mean-square radii	1020:1041	root-mean-square radii from 20 to 140 nm	1020:1059	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	2	19	theme	molecular	304:312	arg1	size					314:317	molecular size	304:317	molecular size	304:317	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	0	20	theme	β-glucan	27:34	arg1	extracts					36:43	cereal β-glucan extracts	20:43	cereal β-glucan extracts from oat and barley	20:63	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	4	21	theme	proteinaceous	731:743	arg1	moieties					745:752	any proteinaceous moieties	727:752	any proteinaceous moieties linked to β-glucan	727:771	Whether there were any proteinaceous moieties linked to β-glucan was also examined.
28196092	7	22	theme	high	956:959	arg1	g/mol					996:1000	105-108 g/mol	988:1000	105-108 g/mol	988:1000	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	7	22	theme	high	956:959	arg1	weight					971:976	high molecular weight β-glucan	956:985	high molecular weight β-glucan (105-108 g/mol)	956:1001	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	7	23	theme	molecular	961:969	arg1	g/mol					996:1000	105-108 g/mol	988:1000	105-108 g/mol	988:1000	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	7	23	theme	molecular	961:969	arg1	weight					971:976	high molecular weight β-glucan	956:985	high molecular weight β-glucan (105-108 g/mol)	956:1001	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	2	24	theme	light	423:427	arg1	MALS					441:444	MALS	441:444	MALS	441:444	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	2	24	theme	light	423:427	arg1	scattering					429:438	multiangle light scattering	412:438	multiangle light scattering (MALS)	412:445	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	0	25	theme	cereal	20:25	arg1	extracts					36:43	cereal β-glucan extracts	20:43	cereal β-glucan extracts from oat and barley	20:63	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	5	26	contain	contained	810:818	arg1	extracts					801:808	Purified extracts	792:808	Purified extracts	792:808	Purified extracts contained 65% and 53% β-glucan for oats and barley, respectively.
28196092	5	26	contain	contained	810:818	arg2	%					830:830	53%	828:830	53% β-glucan for oats and barley	828:859	Purified extracts contained 65% and 53% β-glucan for oats and barley, respectively.
28196092	5	26	contain	contained	810:818	arg2	%					822:822	65%	820:822	65%	820:822	Purified extracts contained 65% and 53% β-glucan for oats and barley, respectively.
28196092	2	27	theme	multiangle	412:421	arg1	MALS					441:444	MALS	441:444	MALS	441:444	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	2	27	theme	multiangle	412:421	arg1	scattering					429:438	multiangle light scattering	412:438	multiangle light scattering (MALS)	412:445	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	0	28	from	quantification	69:82	arg1	barley					58:63	barley	58:63	barley	58:63	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	0	28	from	quantification	69:82	arg1	oat					50:52	oat	50:52	oat	50:52	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	3	29	theme	extracts	553:560	arg1	composition					534:544	The carbohydrate composition	517:544	The carbohydrate composition of the extracts	517:560	The carbohydrate composition of the extracts was analysed using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC).
28196092	0	30	theme	extracts	36:43	arg1	quantification					69:82	quantification	69:82	quantification of proteinaceous matter	69:106	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	0	30	theme	extracts	36:43	arg1	Characterization					0:15	Characterization	0:15	Characterization of cereal β-glucan extracts from oat and barley	0:63	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	2	31	theme	refractive	461:470	arg1	dRI					479:481	dRI	479:481	dRI	479:481	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	2	31	theme	refractive	461:470	arg1	index					472:476	differential refractive index	448:476	differential refractive index (dRI)	448:482	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	3	32	theme	polysaccharide	581:594	arg1	analysis					596:603	polysaccharide analysis	581:603	polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC)	581:705	The carbohydrate composition of the extracts was analysed using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC).
28196092	7	33	theme	large	1007:1011	arg1	sizes					1013:1017	large sizes	1007:1017	large sizes (root-mean-square radii from 20 to 140 nm)	1007:1060	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	8	34	theme	suggested	1134:1142	arg1	functionality					1144:1156	any suggested functionality	1130:1156	any suggested functionality of proteins regarding the health benefits of β-glucan	1130:1210	No proteins covalently bound to β-glucan were detected; therefore, any suggested functionality of proteins regarding the health benefits of β-glucan can be discounted.
28196092	2	35	theme	differential	448:459	arg1	dRI					479:481	dRI	479:481	dRI	479:481	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	2	35	theme	differential	448:459	arg1	index					472:476	differential refractive index	448:476	differential refractive index (dRI)	448:482	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	8	36	theme	health	1184:1189	arg1	benefits					1191:1198	the health benefits	1180:1198	the health benefits of β-glucan	1180:1210	No proteins covalently bound to β-glucan were detected; therefore, any suggested functionality of proteins regarding the health benefits of β-glucan can be discounted.
28196092	0	37	from	barley	58:63	arg1	quantification					69:82	quantification	69:82	quantification of proteinaceous matter	69:106	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	0	37	from	barley	58:63	arg1	Characterization					0:15	Characterization	0:15	Characterization of cereal β-glucan extracts from oat and barley	0:63	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	0	37	from	barley	58:63	arg1	extracts					36:43	cereal β-glucan extracts	20:43	cereal β-glucan extracts from oat and barley	20:63	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
28196092	2	38	theme	distributions	334:346	arg1	terms					295:299	terms	295:299	terms of molecular size and molar mass distributions	295:346	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	1	39	theme	extraction	112:121	arg1	method					123:128	An extraction method	109:128	An extraction method for mixed-linkage β-glucan from oat and barley	109:175	An extraction method for mixed-linkage β-glucan from oat and barley was developed in order to minimize the effect of extraction on the β-glucan structure.
28196092	4	40	attach	linked	754:759	arg1	β-glucan					764:771	β-glucan	764:771	β-glucan	764:771	Whether there were any proteinaceous moieties linked to β-glucan was also examined.
28196092	4	40	attach	linked	754:759	arg2	moieties					745:752	any proteinaceous moieties	727:752	any proteinaceous moieties linked to β-glucan	727:771	Whether there were any proteinaceous moieties linked to β-glucan was also examined.
28196092	3	41	theme	anion-exchange	669:682	arg1	HPAEC					700:704	HPAEC	700:704	HPAEC	700:704	The carbohydrate composition of the extracts was analysed using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC).
28196092	3	41	theme	anion-exchange	669:682	arg1	chromatography					684:697	high-performance anion-exchange chromatography	652:697	high-performance anion-exchange chromatography (HPAEC)	652:705	The carbohydrate composition of the extracts was analysed using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC).
28196092	2	42	theme	mass	329:332	arg1	distributions					334:346	molar mass distributions	323:346	molar mass distributions	323:346	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	6	43	theme	starch	925:930	arg1	impurities					885:894	The main impurities	876:894	The main impurities	876:894	The main impurities were degradation products of starch.
28196092	6	43	theme	starch	925:930	arg1	products					913:920	degradation products	901:920	degradation products of starch	901:930	The main impurities were degradation products of starch.
28196092	1	44	from	effect	216:221	arg1	structure					253:261	the β-glucan structure	240:261	the β-glucan structure	240:261	An extraction method for mixed-linkage β-glucan from oat and barley was developed in order to minimize the effect of extraction on the β-glucan structure.
28196092	3	45	theme	carbohydrate	608:619	arg1	PACE					642:645	PACE	642:645	PACE	642:645	The carbohydrate composition of the extracts was analysed using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC).
28196092	3	45	theme	carbohydrate	608:619	arg1	electrophoresis					625:639	carbohydrate gel electrophoresis	608:639	carbohydrate gel electrophoresis (PACE)	608:646	The carbohydrate composition of the extracts was analysed using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC).
28196092	2	46	theme	molar	323:327	arg1	distributions					334:346	molar mass distributions	323:346	molar mass distributions	323:346	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	7	47	theme	β-glucan	978:985	arg1	g/mol					996:1000	105-108 g/mol	988:1000	105-108 g/mol	988:1000	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	7	47	theme	β-glucan	978:985	arg1	weight					971:976	high molecular weight β-glucan	956:985	high molecular weight β-glucan (105-108 g/mol)	956:1001	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	7	48	theme	root-mean-square	1020:1035	arg1	radii					1037:1041	root-mean-square radii	1020:1041	root-mean-square radii from 20 to 140 nm	1020:1059	The extracts contained high molecular weight β-glucan (105-108 g/mol) and large sizes (root-mean-square radii from 20 to 140 nm).
28196092	3	49	theme	gel	621:623	arg1	PACE					642:645	PACE	642:645	PACE	642:645	The carbohydrate composition of the extracts was analysed using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC).
28196092	3	49	theme	gel	621:623	arg1	electrophoresis					625:639	carbohydrate gel electrophoresis	608:639	carbohydrate gel electrophoresis (PACE)	608:646	The carbohydrate composition of the extracts was analysed using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC).
28196092	1	50	theme	mixed-linkage	134:146	arg1	β-glucan					148:155	mixed-linkage β-glucan	134:155	mixed-linkage β-glucan from oat and barley	134:175	An extraction method for mixed-linkage β-glucan from oat and barley was developed in order to minimize the effect of extraction on the β-glucan structure.
28196092	3	51	theme	high-performance	652:667	arg1	HPAEC					700:704	HPAEC	700:704	HPAEC	700:704	The carbohydrate composition of the extracts was analysed using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC).
28196092	3	51	theme	high-performance	652:667	arg1	chromatography					684:697	high-performance anion-exchange chromatography	652:697	high-performance anion-exchange chromatography (HPAEC)	652:705	The carbohydrate composition of the extracts was analysed using polysaccharide analysis by carbohydrate gel electrophoresis (PACE) and high-performance anion-exchange chromatography (HPAEC).
28196092	2	52	theme	field-flow	370:379	arg1	AF4					396:398	AF4	396:398	AF4	396:398	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	2	52	theme	field-flow	370:379	arg1	fractionation					381:393	asymmetric flow field-flow fractionation	354:393	asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection	354:514	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	1	53	theme	extraction	226:235	arg1	effect					216:221	the effect	212:221	the effect of extraction on the β-glucan structure	212:261	An extraction method for mixed-linkage β-glucan from oat and barley was developed in order to minimize the effect of extraction on the β-glucan structure.
28196092	8	54	theme	β-glucan	1203:1210	arg1	benefits					1191:1198	the health benefits	1180:1198	the health benefits of β-glucan	1180:1210	No proteins covalently bound to β-glucan were detected; therefore, any suggested functionality of proteins regarding the health benefits of β-glucan can be discounted.
28196092	1	55	from	barley	170:175	arg1	β-glucan					148:155	mixed-linkage β-glucan	134:155	mixed-linkage β-glucan from oat and barley	134:175	An extraction method for mixed-linkage β-glucan from oat and barley was developed in order to minimize the effect of extraction on the β-glucan structure.
28196092	2	56	theme	flow	365:368	arg1	AF4					396:398	AF4	396:398	AF4	396:398	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	2	56	theme	flow	365:368	arg1	fractionation					381:393	asymmetric flow field-flow fractionation	354:393	asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection	354:514	β-Glucan were characterized in terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection.
28196092	0	57	theme	proteinaceous	87:99	arg1	matter					101:106	proteinaceous matter	87:106	proteinaceous matter	87:106	Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter.
27624316	7	0	theme	surface	1163:1169	arg1	tool					1207:1210	a quality assurance tool	1187:1210	a quality assurance tool	1187:1210	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	7	0	theme	surface	1163:1169	arg1	interactions					1171:1182	their surface interactions	1157:1182	their surface interactions	1157:1182	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	7	0	theme	surface	1163:1169	arg1	nanoparticles					1139:1151	nanoparticles	1139:1151	nanoparticles	1139:1151	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	4	1	theme	ZnO	580:582	arg1	NP					584:585	ZnO NP	580:585	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG)	580:676	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift.
27624316	6	2	theme	fluorescent	993:1003	arg1	intensity					1005:1013	fluorescent intensity	993:1013	fluorescent intensity	993:1013	Ras-Binding domain (RBD) at three concentrations (25, 37.5, 50 μg/mL) showed that fluorescent intensity was inversely related to the concentration of protein loaded.
27624316	7	3	theme	quality	1189:1195	arg1	tool					1207:1210	a quality assurance tool	1187:1210	a quality assurance tool	1187:1210	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	7	3	theme	quality	1189:1195	arg1	interactions					1171:1182	their surface interactions	1157:1182	their surface interactions	1157:1182	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	7	3	theme	quality	1189:1195	arg1	nanoparticles					1139:1151	nanoparticles	1139:1151	nanoparticles	1139:1151	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	1	4	theme	fluorescent	305:315	arg1	intensity					317:325	the fluorescent intensity	301:325	the fluorescent intensity per excitation and emission wavelengths	301:365	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	5	5	theme	polycytidylic	792:804	arg1	pIC					812:814	pIC	812:814	pIC	812:814	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	5	theme	polycytidylic	792:804	arg1	680 nm					817:822	680 nm	817:822	680 nm	817:822	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	5	theme	polycytidylic	792:804	arg1	acid					806:809	polycytidylic acid	792:809	polyinosinic: polycytidylic acid (pIC)(680 nm)	778:823	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	7	6	theme	assurance	1197:1205	arg1	tool					1207:1210	a quality assurance tool	1187:1210	a quality assurance tool	1187:1210	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	7	6	theme	assurance	1197:1205	arg1	interactions					1171:1182	their surface interactions	1157:1182	their surface interactions	1157:1182	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	7	6	theme	assurance	1197:1205	arg1	nanoparticles					1139:1151	nanoparticles	1139:1151	nanoparticles	1139:1151	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	1	7	theme	intensity	317:325	arg1	signature					288:296	a spectral signature	277:296	a spectral signature of the fluorescent intensity per excitation and emission wavelengths	277:365	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	4	8	theme	polyethylene	651:662	arg1	mPEG					672:675	mPEG	672:675	mPEG	672:675	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift.
27624316	4	8	theme	polyethylene	651:662	arg1	glycol					664:669	methoxy polyethylene glycol	643:669	methoxy polyethylene glycol (mPEG)	643:676	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift.
27624316	5	9	theme	ZnO	725:727	arg1	NP					729:730	ZnO NP	725:730	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each	725:879	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	6	10	theme	Ras-Binding	911:921	arg1	domain					923:928	Ras-Binding domain	911:928	Ras-Binding domain (RBD) at three concentrations (25, 37.5, 50 μg/mL)	911:979	Ras-Binding domain (RBD) at three concentrations (25, 37.5, 50 μg/mL) showed that fluorescent intensity was inversely related to the concentration of protein loaded.
27624316	6	10	theme	Ras-Binding	911:921	arg1	RBD					931:933	RBD	931:933	RBD	931:933	Ras-Binding domain (RBD) at three concentrations (25, 37.5, 50 μg/mL) showed that fluorescent intensity was inversely related to the concentration of protein loaded.
27624316	1	11	theme	excitation	331:340	arg1	wavelengths					355:365	excitation and emission wavelengths	331:365	excitation and emission wavelengths	331:365	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	7	12	theme	novel	1108:1112	arg1	FDS					1099:1101	2D FDS	1096:1101	2D FDS	1096:1101	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	7	12	theme	novel	1108:1112	arg1	technique					1114:1122	a novel technique	1106:1122	a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool	1106:1210	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	5	13	theme	splice	829:834	arg1	SSO					863:865	SSO	863:865	SSO	863:865	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	13	theme	splice	829:834	arg1	650 nm					868:873	650 nm	868:873	650 nm	868:873	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	13	theme	splice	829:834	arg1	oligonucleotide					846:860	splice switching oligonucleotide	829:860	splice switching oligonucleotide (SSO)(650 nm) each	829:879	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	1	14	theme	surface	213:219	arg1	functionalization					221:237	surface functionalization	213:237	surface functionalization	213:237	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	4	15	theme	methoxy	643:649	arg1	mPEG					672:675	mPEG	672:675	mPEG	672:675	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift.
27624316	4	15	theme	methoxy	643:649	arg1	glycol					664:669	methoxy polyethylene glycol	643:669	methoxy polyethylene glycol (mPEG)	643:676	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift.
27624316	6	16	from	concentrations	945:958	arg1	domain					923:928	Ras-Binding domain	911:928	Ras-Binding domain (RBD) at three concentrations (25, 37.5, 50 μg/mL)	911:979	Ras-Binding domain (RBD) at three concentrations (25, 37.5, 50 μg/mL) showed that fluorescent intensity was inversely related to the concentration of protein loaded.
27624316	6	16	from	concentrations	945:958	arg1	RBD					931:933	RBD	931:933	RBD	931:933	Ras-Binding domain (RBD) at three concentrations (25, 37.5, 50 μg/mL) showed that fluorescent intensity was inversely related to the concentration of protein loaded.
27624316	6	17	dep	concentrations	945:958	arg1	25					961:962	25	961:962	25	961:962	Ras-Binding domain (RBD) at three concentrations (25, 37.5, 50 μg/mL) showed that fluorescent intensity was inversely related to the concentration of protein loaded.
27624316	6	17	dep	concentrations	945:958	arg1	50 μg/mL					971:978	50 μg/mL	971:978	50 μg/mL	971:978	Ras-Binding domain (RBD) at three concentrations (25, 37.5, 50 μg/mL) showed that fluorescent intensity was inversely related to the concentration of protein loaded.
27624316	4	18	theme	signature	708:716	arg1	shift					718:722	a distinct spectral signature shift	688:722	a distinct spectral signature shift	688:722	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift.
27624316	0	19	theme	Fluorescence	16:27	arg1	Spectroscopy					40:51	Two-Dimensional Fluorescence Difference Spectroscopy	0:51	Two-Dimensional Fluorescence Difference Spectroscopy	0:51	Two-Dimensional Fluorescence Difference Spectroscopy to Characterize Nanoparticles and their Interactions.
27624316	1	20	theme	dimensional	111:121	arg1	FDS					164:166	2D FDS	161:166	2D FDS	161:166	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	1	20	theme	dimensional	111:121	arg1	spectroscopy					147:158	Two dimensional fluorescence difference spectroscopy	107:158	Two dimensional fluorescence difference spectroscopy (2D FDS)	107:167	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	0	21	theme	Two-Dimensional	0:14	arg1	Spectroscopy					40:51	Two-Dimensional Fluorescence Difference Spectroscopy	0:51	Two-Dimensional Fluorescence Difference Spectroscopy	0:51	Two-Dimensional Fluorescence Difference Spectroscopy to Characterize Nanoparticles and their Interactions.
27624316	2	22	theme	oxide	384:388	arg1	nanoparticles					390:402	Comparing metal oxide nanoparticles	368:402	Comparing metal oxide nanoparticles	368:402	Comparing metal oxide nanoparticles revealed a unique spectral signature per material composition.
27624316	1	23	theme	biomolecule	243:253	arg1	loading					255:261	biomolecule loading	243:261	biomolecule loading	243:261	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	1	24	theme	emission	346:353	arg1	wavelengths					355:365	excitation and emission wavelengths	331:365	excitation and emission wavelengths	331:365	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	5	25	theme	Yeast	751:755	arg1	640 nm					769:774	640 nm	769:774	640 nm	769:774	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	25	theme	Yeast	751:755	arg1	RNA					757:759	Torula Yeast RNA	744:759	Torula Yeast RNA (TYRNA)(640 nm)	744:775	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	25	theme	Yeast	751:755	arg1	TYRNA					762:766	TYRNA	762:766	TYRNA	762:766	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	26	from	shift	892:896	arg1	emission					901:908	emission	901:908	emission	901:908	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	2	27	theme	metal	378:382	arg1	nanoparticles					390:402	Comparing metal oxide nanoparticles	368:402	Comparing metal oxide nanoparticles	368:402	Comparing metal oxide nanoparticles revealed a unique spectral signature per material composition.
27624316	1	28	theme	fluorescence	123:134	arg1	FDS					164:166	2D FDS	161:166	2D FDS	161:166	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	1	28	theme	fluorescence	123:134	arg1	spectroscopy					147:158	Two dimensional fluorescence difference spectroscopy	107:158	Two dimensional fluorescence difference spectroscopy (2D FDS)	107:167	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	2	29	theme	Comparing	368:376	arg1	nanoparticles					390:402	Comparing metal oxide nanoparticles	368:402	Comparing metal oxide nanoparticles	368:402	Comparing metal oxide nanoparticles revealed a unique spectral signature per material composition.
27624316	3	30	theme	2D	467:468	arg1	FDS					470:472	2D FDS	467:472	2D FDS	467:472	2D FDS showed to be sensitive to changes in surface properties between ZnO NPs synthesized by different methods.
27624316	5	31	theme	switching	836:844	arg1	SSO					863:865	SSO	863:865	SSO	863:865	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	31	theme	switching	836:844	arg1	650 nm					868:873	650 nm	868:873	650 nm	868:873	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	31	theme	switching	836:844	arg1	oligonucleotide					846:860	splice switching oligonucleotide	829:860	splice switching oligonucleotide (SSO)(650 nm) each	829:879	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	4	32	theme	spectral	699:706	arg1	shift					718:722	a distinct spectral signature shift	688:722	a distinct spectral signature shift	688:722	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift.
27624316	3	33	theme	surface	511:517	arg1	properties					519:528	surface properties	511:528	surface properties between ZnO NPs synthesized by different methods	511:577	2D FDS showed to be sensitive to changes in surface properties between ZnO NPs synthesized by different methods.
27624316	5	34	dep	polyinosinic	778:789	arg1	pIC					812:814	pIC	812:814	pIC	812:814	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	34	dep	polyinosinic	778:789	arg1	680 nm					817:822	680 nm	817:822	680 nm	817:822	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	34	dep	polyinosinic	778:789	arg1	acid					806:809	polycytidylic acid	792:809	polyinosinic: polycytidylic acid (pIC)(680 nm)	778:823	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	3	35	theme	ZnO	538:540	arg1	NPs					542:544	ZnO NPs	538:544	ZnO NPs synthesized by different methods	538:577	2D FDS showed to be sensitive to changes in surface properties between ZnO NPs synthesized by different methods.
27624316	4	36	theme	glycol	599:604	arg1	chitosan					606:613	glycol chitosan	599:613	glycol chitosan	599:613	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift.
27624316	2	37	theme	spectral	422:429	arg1	signature					431:439	a unique spectral signature	413:439	a unique spectral signature per material composition	413:464	Comparing metal oxide nanoparticles revealed a unique spectral signature per material composition.
27624316	7	38	theme	2D	1096:1097	arg1	FDS					1099:1101	2D FDS	1096:1101	2D FDS	1096:1101	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	7	38	theme	2D	1096:1097	arg1	technique					1114:1122	a novel technique	1106:1122	a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool	1106:1210	These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool.
27624316	4	39	theme	polyacrylic	616:626	arg1	PAA					634:636	PAA	634:636	PAA	634:636	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift.
27624316	4	39	theme	polyacrylic	616:626	arg1	acid					628:631	polyacrylic acid	616:631	polyacrylic acid (PAA)	616:637	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift.
27624316	2	40	theme	unique	415:420	arg1	signature					431:439	a unique spectral signature	413:439	a unique spectral signature per material composition	413:464	Comparing metal oxide nanoparticles revealed a unique spectral signature per material composition.
27624316	1	41	theme	difference	136:145	arg1	FDS					164:166	2D FDS	161:166	2D FDS	161:166	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	1	41	theme	difference	136:145	arg1	spectroscopy					147:158	Two dimensional fluorescence difference spectroscopy	107:158	Two dimensional fluorescence difference spectroscopy (2D FDS)	107:167	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	1	42	theme	spectral	279:286	arg1	signature					288:296	a spectral signature	277:296	a spectral signature of the fluorescent intensity per excitation and emission wavelengths	277:365	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	5	43	theme	Torula	744:749	arg1	640 nm					769:774	640 nm	769:774	640 nm	769:774	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	43	theme	Torula	744:749	arg1	RNA					757:759	Torula Yeast RNA	744:759	Torula Yeast RNA (TYRNA)(640 nm)	744:775	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	43	theme	Torula	744:749	arg1	TYRNA					762:766	TYRNA	762:766	TYRNA	762:766	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	5	44	dep	oligonucleotide	846:860	arg1	each					876:879	each	876:879	each	876:879	ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission.
27624316	3	45	from	changes	500:506	arg1	properties					519:528	surface properties	511:528	surface properties between ZnO NPs synthesized by different methods	511:577	2D FDS showed to be sensitive to changes in surface properties between ZnO NPs synthesized by different methods.
27624316	1	46	theme	nanoparticle	177:188	arg1	interactions					190:201	nanoparticle interactions	177:201	nanoparticle interactions	177:201	Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths.
27624316	4	47	theme	distinct	690:697	arg1	shift					718:722	a distinct spectral signature shift	688:722	a distinct spectral signature shift	688:722	ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift.
27624316	6	48	theme	protein	1061:1067	arg1	concentration					1044:1056	the concentration	1040:1056	the concentration of protein loaded	1040:1074	Ras-Binding domain (RBD) at three concentrations (25, 37.5, 50 μg/mL) showed that fluorescent intensity was inversely related to the concentration of protein loaded.
27624316	3	49	theme	different	561:569	arg1	methods					571:577	different methods	561:577	different methods	561:577	2D FDS showed to be sensitive to changes in surface properties between ZnO NPs synthesized by different methods.
27624316	0	50	theme	Difference	29:38	arg1	Spectroscopy					40:51	Two-Dimensional Fluorescence Difference Spectroscopy	0:51	Two-Dimensional Fluorescence Difference Spectroscopy	0:51	Two-Dimensional Fluorescence Difference Spectroscopy to Characterize Nanoparticles and their Interactions.
27624316	2	51	theme	material	445:452	arg1	composition					454:464	material composition	445:464	material composition	445:464	Comparing metal oxide nanoparticles revealed a unique spectral signature per material composition.
26896534	6	0	theme	northern	1281:1288	arg1	controls					1251:1258	positive controls	1242:1258	positive controls (southern yellow and northern pine)	1242:1294	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	0	theme	northern	1281:1288	arg1	pine					1290:1293	northern pine	1281:1293	northern pine	1281:1293	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	1	theme	pine	1228:1231	arg1	woods					1233:1237	two natural pine woods	1216:1237	two natural pine woods as positive controls (southern yellow and northern pine)	1216:1294	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	9	2	theme	nutrition-based	1735:1749	arg1	factors					1751:1757	new nutrition-based factors	1731:1757	new nutrition-based factors that can potentially impact bait feeding, trophallactic exchange, and efficacy	1731:1836	These findings thus illuminate new nutrition-based factors that can potentially impact bait feeding, trophallactic exchange, and efficacy.
26896534	4	3	contain	have	712:715	arg1	baits					674:678	termite baits	666:678	termite baits that are more digestible	666:703	The rationale behind this study is that termite baits that are more digestible should have more nutritional value to termites and thus encourage maximal feeding and trophallactic transfer of active ingredients through termite colonies.
26896534	4	3	contain	have	712:715	arg2	value					734:738	more nutritional value	717:738	more nutritional value	717:738	The rationale behind this study is that termite baits that are more digestible should have more nutritional value to termites and thus encourage maximal feeding and trophallactic transfer of active ingredients through termite colonies.
26896534	3	4	theme	matrix	536:541	arg1	components					543:552	matrix components	536:552	matrix components	536:552	This study took a novel approach for assessing digestibility of termite bait matrices and matrix components to gain potentially new insights into bait attractiveness and efficacy.
26896534	1	5	theme	wooden	250:255	arg1	structures					257:266	wooden structures	250:266	wooden structures	250:266	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	9	6	theme	new	1731:1733	arg1	factors					1751:1757	new nutrition-based factors	1731:1757	new nutrition-based factors that can potentially impact bait feeding, trophallactic exchange, and efficacy	1731:1836	These findings thus illuminate new nutrition-based factors that can potentially impact bait feeding, trophallactic exchange, and efficacy.
26896534	7	7	theme	sufficient	1439:1448	arg1	value					1462:1466	sufficient nutritional value	1439:1466	sufficient nutritional value for the proprietary matrices	1439:1495	Overall results show equal or greater monosaccharide availability for some commercial matrices than standard pine lignocelluloses, suggesting sufficient nutritional value for the proprietary matrices.
26896534	3	8	theme	matrices	523:530	arg1	digestibility					493:505	digestibility	493:505	digestibility of termite bait matrices and matrix components	493:552	This study took a novel approach for assessing digestibility of termite bait matrices and matrix components to gain potentially new insights into bait attractiveness and efficacy.
26896534	7	9	theme	proprietary	1476:1486	arg1	matrices					1488:1495	the proprietary matrices	1472:1495	the proprietary matrices	1472:1495	Overall results show equal or greater monosaccharide availability for some commercial matrices than standard pine lignocelluloses, suggesting sufficient nutritional value for the proprietary matrices.
26896534	6	10	theme	compressed	1166:1175	arg1	components					1154:1163	two matrix components	1143:1163	two matrix components (compressed and toasted compressed cellulose)	1143:1209	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	10	theme	compressed	1166:1175	arg1	cellulose					1200:1208	compressed and toasted compressed cellulose	1166:1208	compressed and toasted compressed cellulose	1166:1208	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	4	11	theme	termite	666:672	arg1	baits					674:678	termite baits	666:678	termite baits that are more digestible	666:703	The rationale behind this study is that termite baits that are more digestible should have more nutritional value to termites and thus encourage maximal feeding and trophallactic transfer of active ingredients through termite colonies.
26896534	8	12	from	differences	1625:1635	arg1	adaptation					1679:1688	long-term diet adaptation	1664:1688	long-term diet adaptation	1664:1688	Another more prominent trend was significant intercolony variation in digestibility across substrates, possibly resulting from differences in microbiota composition, long-term diet adaptation, or both.
26896534	8	12	from	differences	1625:1635	arg1	both					1694:1697	both	1694:1697	both	1694:1697	Another more prominent trend was significant intercolony variation in digestibility across substrates, possibly resulting from differences in microbiota composition, long-term diet adaptation, or both.
26896534	8	12	from	differences	1625:1635	arg1	composition					1651:1661	microbiota composition	1640:1661	microbiota composition	1640:1661	Another more prominent trend was significant intercolony variation in digestibility across substrates, possibly resulting from differences in microbiota composition, long-term diet adaptation, or both.
26896534	5	13	theme	in	886:887	arg1	assays					905:910	in vitro digestion assays	886:910	in vitro digestion assays	886:910	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	7	14	theme	pine	1406:1409	arg1	lignocelluloses					1411:1425	standard pine lignocelluloses	1397:1425	standard pine lignocelluloses	1397:1425	Overall results show equal or greater monosaccharide availability for some commercial matrices than standard pine lignocelluloses, suggesting sufficient nutritional value for the proprietary matrices.
26896534	2	15	theme	same	277:280	arg1	reason					282:287	the same reason	273:287	the same reason	273:287	For the same reason, termite baits that combine cellulosic matrices with slow-acting insecticides are both effective and popular as a reduced-risk approach for termite control.
26896534	5	16	from	substrates	1039:1048	arg1	detection					971:979	colorimetric detection	958:979	colorimetric detection of released glucose and pentose monosaccharides from test substrates	958:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	5	16	from	substrates	1039:1048	arg1	monosaccharides					1013:1027	released glucose and pentose monosaccharides	984:1027	released glucose and pentose monosaccharides from test substrates	984:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	1	17	theme	central	187:193	arg1	digesters					142:150	highly effective digesters	125:150	highly effective digesters	125:150	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	1	17	theme	central	187:193	arg1	factor					195:200	a central factor	185:200	a central factor contributing to their global status as pests of wooden structures	185:266	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	3	18	theme	bait	592:595	arg1	attractiveness					597:610	bait attractiveness	592:610	bait attractiveness	592:610	This study took a novel approach for assessing digestibility of termite bait matrices and matrix components to gain potentially new insights into bait attractiveness and efficacy.
26896534	7	19	theme	commercial	1372:1381	arg1	matrices					1383:1390	some commercial matrices	1367:1390	some commercial matrices	1367:1390	Overall results show equal or greater monosaccharide availability for some commercial matrices than standard pine lignocelluloses, suggesting sufficient nutritional value for the proprietary matrices.
26896534	5	20	theme	gut	925:927	arg1	extracts					937:944	termite gut protein extracts	917:944	termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates	917:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	9	21	theme	trophallactic	1801:1813	arg1	exchange					1815:1822	trophallactic exchange	1801:1822	trophallactic exchange	1801:1822	These findings thus illuminate new nutrition-based factors that can potentially impact bait feeding, trophallactic exchange, and efficacy.
26896534	4	22	theme	more	717:720	arg1	value					734:738	more nutritional value	717:738	more nutritional value	717:738	The rationale behind this study is that termite baits that are more digestible should have more nutritional value to termites and thus encourage maximal feeding and trophallactic transfer of active ingredients through termite colonies.
26896534	8	23	theme	prominent	1511:1519	arg1	trend					1521:1525	Another more prominent trend	1498:1525	Another more prominent trend	1498:1525	Another more prominent trend was significant intercolony variation in digestibility across substrates, possibly resulting from differences in microbiota composition, long-term diet adaptation, or both.
26896534	7	24	theme	monosaccharide	1335:1348	arg1	availability					1350:1361	monosaccharide availability	1335:1361	monosaccharide availability	1335:1361	Overall results show equal or greater monosaccharide availability for some commercial matrices than standard pine lignocelluloses, suggesting sufficient nutritional value for the proprietary matrices.
26896534	6	25	theme	commercial	1086:1095	arg1	matrices					1102:1109	two commercial bait matrices	1082:1109	two commercial bait matrices (Recruit IV and Recruit II HD)	1082:1140	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	0	26	theme	Termite	51:57	arg1	flavipes					74:81	the Pest Termite Reticulitermes flavipes	42:81	the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae)	42:109	Digestion of Termiticide Bait Matrices by the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae).
26896534	5	27	theme	digestion	895:903	arg1	assays					905:910	in vitro digestion assays	886:910	in vitro digestion assays	886:910	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	1	28	theme	wood	155:158	arg1	lignocellulose					160:173	wood lignocellulose	155:173	wood lignocellulose	155:173	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	8	29	theme	intercolony	1543:1553	arg1	variation					1555:1563	significant intercolony variation	1531:1563	significant intercolony variation	1531:1563	Another more prominent trend was significant intercolony variation in digestibility across substrates, possibly resulting from differences in microbiota composition, long-term diet adaptation, or both.
26896534	5	30	theme	released	984:991	arg1	monosaccharides					1013:1027	released glucose and pentose monosaccharides	984:1027	released glucose and pentose monosaccharides from test substrates	984:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	4	31	theme	maximal	771:777	arg1	feeding					779:785	maximal feeding	771:785	maximal feeding	771:785	The rationale behind this study is that termite baits that are more digestible should have more nutritional value to termites and thus encourage maximal feeding and trophallactic transfer of active ingredients through termite colonies.
26896534	0	32	dep	Isoptera	84:91	arg1	Rhinotermitidae					94:108	Rhinotermitidae	94:108	Rhinotermitidae	94:108	Digestion of Termiticide Bait Matrices by the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae).
26896534	6	33	dep	matrices	1102:1109	arg1	Recruit					1127:1133	Recruit	1127:1133	Recruit II HD	1127:1139	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	33	dep	matrices	1102:1109	arg1	Recruit					1112:1118	Recruit	1112:1118	Recruit IV	1112:1121	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	1	34	theme	structures	257:266	arg1	pests					241:245	pests	241:245	pests of wooden structures	241:266	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	0	35	dep	flavipes	74:81	arg1	Isoptera					84:91	Isoptera	84:91	Isoptera	84:91	Digestion of Termiticide Bait Matrices by the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae).
26896534	6	36	theme	bait	1097:1100	arg1	matrices					1102:1109	two commercial bait matrices	1082:1109	two commercial bait matrices (Recruit IV and Recruit II HD)	1082:1140	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	2	37	theme	reduced-risk	403:414	arg1	approach					416:423	a reduced-risk approach	401:423	a reduced-risk approach for termite control	401:443	For the same reason, termite baits that combine cellulosic matrices with slow-acting insecticides are both effective and popular as a reduced-risk approach for termite control.
26896534	0	38	theme	Termiticide	13:23	arg1	Matrices					30:37	Termiticide Bait Matrices	13:37	Termiticide Bait Matrices	13:37	Digestion of Termiticide Bait Matrices by the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae).
26896534	6	39	theme	toasted	1181:1187	arg1	components					1154:1163	two matrix components	1143:1163	two matrix components (compressed and toasted compressed cellulose)	1143:1209	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	39	theme	toasted	1181:1187	arg1	cellulose					1200:1208	compressed and toasted compressed cellulose	1166:1208	compressed and toasted compressed cellulose	1166:1208	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	4	40	theme	active	817:822	arg1	ingredients					824:834	active ingredients	817:834	active ingredients	817:834	The rationale behind this study is that termite baits that are more digestible should have more nutritional value to termites and thus encourage maximal feeding and trophallactic transfer of active ingredients through termite colonies.
26896534	0	41	theme	Matrices	30:37	arg1	Digestion					0:8	Digestion	0:8	Digestion of Termiticide Bait Matrices by the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae).	0:110	Digestion of Termiticide Bait Matrices by the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae).
26896534	2	42	theme	cellulosic	317:326	arg1	matrices					328:335	cellulosic matrices	317:335	cellulosic matrices	317:335	For the same reason, termite baits that combine cellulosic matrices with slow-acting insecticides are both effective and popular as a reduced-risk approach for termite control.
26896534	7	43	dep	greater	1327:1333	arg1	availability					1350:1361	monosaccharide availability	1335:1361	monosaccharide availability	1335:1361	Overall results show equal or greater monosaccharide availability for some commercial matrices than standard pine lignocelluloses, suggesting sufficient nutritional value for the proprietary matrices.
26896534	8	44	theme	microbiota	1640:1649	arg1	composition					1651:1661	microbiota composition	1640:1661	microbiota composition	1640:1661	Another more prominent trend was significant intercolony variation in digestibility across substrates, possibly resulting from differences in microbiota composition, long-term diet adaptation, or both.
26896534	5	45	from	detection	971:979	arg1	substrates					1039:1048	test substrates	1034:1048	test substrates	1034:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	5	46	theme	monosaccharides	1013:1027	arg1	detection					971:979	colorimetric detection	958:979	colorimetric detection of released glucose and pentose monosaccharides from test substrates	958:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	1	47	theme	global	224:229	arg1	status					231:236	their global status	218:236	their global status as pests of wooden structures	218:266	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	5	48	theme	test	1034:1037	arg1	substrates					1039:1048	test substrates	1034:1048	test substrates	1034:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	8	49	theme	diet	1674:1677	arg1	adaptation					1679:1688	long-term diet adaptation	1664:1688	long-term diet adaptation	1664:1688	Another more prominent trend was significant intercolony variation in digestibility across substrates, possibly resulting from differences in microbiota composition, long-term diet adaptation, or both.
26896534	6	50	theme	compressed	1189:1198	arg1	components					1154:1163	two matrix components	1143:1163	two matrix components (compressed and toasted compressed cellulose)	1143:1209	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	50	theme	compressed	1189:1198	arg1	cellulose					1200:1208	compressed and toasted compressed cellulose	1166:1208	compressed and toasted compressed cellulose	1166:1208	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	51	theme	southern	1261:1268	arg1	controls					1251:1258	positive controls	1242:1258	positive controls (southern yellow and northern pine)	1242:1294	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	51	theme	southern	1261:1268	arg1	yellow					1270:1275	southern yellow	1261:1275	southern yellow	1261:1275	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	3	52	theme	bait	518:521	arg1	matrices					523:530	termite bait matrices	510:530	termite bait matrices	510:530	This study took a novel approach for assessing digestibility of termite bait matrices and matrix components to gain potentially new insights into bait attractiveness and efficacy.
26896534	1	53	theme	lignocellulose	160:173	arg1	digesters					142:150	highly effective digesters	125:150	highly effective digesters	125:150	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	1	53	theme	lignocellulose	160:173	arg1	factor					195:200	a central factor	185:200	a central factor contributing to their global status as pests of wooden structures	185:266	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	1	53	theme	lignocellulose	160:173	arg1	Termites					112:119	Termites	112:119	Termites	112:119	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	4	54	theme	termite	844:850	arg1	colonies					852:859	termite colonies	844:859	termite colonies	844:859	The rationale behind this study is that termite baits that are more digestible should have more nutritional value to termites and thus encourage maximal feeding and trophallactic transfer of active ingredients through termite colonies.
26896534	7	55	theme	nutritional	1450:1460	arg1	value					1462:1466	sufficient nutritional value	1439:1466	sufficient nutritional value for the proprietary matrices	1439:1495	Overall results show equal or greater monosaccharide availability for some commercial matrices than standard pine lignocelluloses, suggesting sufficient nutritional value for the proprietary matrices.
26896534	2	56	theme	termite	290:296	arg1	baits					298:302	termite baits	290:302	termite baits that combine cellulosic matrices with slow-acting insecticides	290:365	For the same reason, termite baits that combine cellulosic matrices with slow-acting insecticides are both effective and popular as a reduced-risk approach for termite control.
26896534	6	57	theme	positive	1242:1249	arg1	controls					1251:1258	positive controls	1242:1258	positive controls (southern yellow and northern pine)	1242:1294	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	57	theme	positive	1242:1249	arg1	yellow					1270:1275	southern yellow	1261:1275	southern yellow	1261:1275	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	57	theme	positive	1242:1249	arg1	pine					1290:1293	northern pine	1281:1293	northern pine	1281:1293	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	3	58	theme	components	543:552	arg1	digestibility					493:505	digestibility	493:505	digestibility of termite bait matrices and matrix components	493:552	This study took a novel approach for assessing digestibility of termite bait matrices and matrix components to gain potentially new insights into bait attractiveness and efficacy.
26896534	6	59	theme	matrix	1147:1152	arg1	components					1154:1163	two matrix components	1143:1163	two matrix components (compressed and toasted compressed cellulose)	1143:1209	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	59	theme	matrix	1147:1152	arg1	cellulose					1200:1208	compressed and toasted compressed cellulose	1166:1208	compressed and toasted compressed cellulose	1166:1208	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	9	60	theme	bait	1787:1790	arg1	feeding					1792:1798	bait feeding	1787:1798	bait feeding	1787:1798	These findings thus illuminate new nutrition-based factors that can potentially impact bait feeding, trophallactic exchange, and efficacy.
26896534	7	61	theme	standard	1397:1404	arg1	lignocelluloses					1411:1425	standard pine lignocelluloses	1397:1425	standard pine lignocelluloses	1397:1425	Overall results show equal or greater monosaccharide availability for some commercial matrices than standard pine lignocelluloses, suggesting sufficient nutritional value for the proprietary matrices.
26896534	3	62	theme	new	574:576	arg1	insights					578:585	potentially new insights	562:585	potentially new insights into bait attractiveness and efficacy	562:623	This study took a novel approach for assessing digestibility of termite bait matrices and matrix components to gain potentially new insights into bait attractiveness and efficacy.
26896534	5	63	theme	termite	917:923	arg1	extracts					937:944	termite gut protein extracts	917:944	termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates	917:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	6	64	theme	natural	1220:1226	arg1	woods					1233:1237	two natural pine woods	1216:1237	two natural pine woods as positive controls (southern yellow and northern pine)	1216:1294	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	5	65	theme	protein	929:935	arg1	extracts					937:944	termite gut protein extracts	917:944	termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates	917:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	0	66	theme	Reticulitermes	59:72	arg1	flavipes					74:81	the Pest Termite Reticulitermes flavipes	42:81	the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae)	42:109	Digestion of Termiticide Bait Matrices by the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae).
26896534	1	67	theme	effective	132:140	arg1	digesters					142:150	highly effective digesters	125:150	highly effective digesters	125:150	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	1	67	theme	effective	132:140	arg1	factor					195:200	a central factor	185:200	a central factor contributing to their global status as pests of wooden structures	185:266	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	1	67	theme	effective	132:140	arg1	Termites					112:119	Termites	112:119	Termites	112:119	Termites are highly effective digesters of wood lignocellulose, which is a central factor contributing to their global status as pests of wooden structures.
26896534	8	68	theme	significant	1531:1541	arg1	variation					1555:1563	significant intercolony variation	1531:1563	significant intercolony variation	1531:1563	Another more prominent trend was significant intercolony variation in digestibility across substrates, possibly resulting from differences in microbiota composition, long-term diet adaptation, or both.
26896534	5	69	theme	glucose	993:999	arg1	monosaccharides					1013:1027	released glucose and pentose monosaccharides	984:1027	released glucose and pentose monosaccharides from test substrates	984:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	8	70	from	variation	1555:1563	arg1	digestibility					1568:1580	digestibility	1568:1580	digestibility across substrates	1568:1598	Another more prominent trend was significant intercolony variation in digestibility across substrates, possibly resulting from differences in microbiota composition, long-term diet adaptation, or both.
26896534	2	71	theme	termite	429:435	arg1	control					437:443	termite control	429:443	termite control	429:443	For the same reason, termite baits that combine cellulosic matrices with slow-acting insecticides are both effective and popular as a reduced-risk approach for termite control.
26896534	4	72	theme	nutritional	722:732	arg1	value					734:738	more nutritional value	717:738	more nutritional value	717:738	The rationale behind this study is that termite baits that are more digestible should have more nutritional value to termites and thus encourage maximal feeding and trophallactic transfer of active ingredients through termite colonies.
26896534	0	73	theme	Bait	25:28	arg1	Matrices					30:37	Termiticide Bait Matrices	13:37	Termiticide Bait Matrices	13:37	Digestion of Termiticide Bait Matrices by the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae).
26896534	5	74	theme	colorimetric	958:969	arg1	detection					971:979	colorimetric detection	958:979	colorimetric detection of released glucose and pentose monosaccharides from test substrates	958:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	7	75	theme	Overall	1297:1303	arg1	results					1305:1311	Overall results	1297:1311	Overall results	1297:1311	Overall results show equal or greater monosaccharide availability for some commercial matrices than standard pine lignocelluloses, suggesting sufficient nutritional value for the proprietary matrices.
26896534	5	76	dep	in	886:887	arg1	vitro					889:893	vitro	889:893	vitro	889:893	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	0	77	theme	Pest	46:49	arg1	flavipes					74:81	the Pest Termite Reticulitermes flavipes	42:81	the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae)	42:109	Digestion of Termiticide Bait Matrices by the Pest Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae).
26896534	2	78	theme	slow-acting	342:352	arg1	insecticides					354:365	slow-acting insecticides	342:365	slow-acting insecticides	342:365	For the same reason, termite baits that combine cellulosic matrices with slow-acting insecticides are both effective and popular as a reduced-risk approach for termite control.
26896534	4	79	theme	ingredients	824:834	arg1	feeding					779:785	maximal feeding	771:785	maximal feeding	771:785	The rationale behind this study is that termite baits that are more digestible should have more nutritional value to termites and thus encourage maximal feeding and trophallactic transfer of active ingredients through termite colonies.
26896534	4	79	theme	ingredients	824:834	arg1	transfer					805:812	trophallactic transfer	791:812	trophallactic transfer of active ingredients through termite colonies	791:859	The rationale behind this study is that termite baits that are more digestible should have more nutritional value to termites and thus encourage maximal feeding and trophallactic transfer of active ingredients through termite colonies.
26896534	5	80	theme	pentose	1005:1011	arg1	monosaccharides					1013:1027	released glucose and pentose monosaccharides	984:1027	released glucose and pentose monosaccharides from test substrates	984:1048	Studies were done using in vitro digestion assays with termite gut protein extracts followed by colorimetric detection of released glucose and pentose monosaccharides from test substrates.
26896534	8	81	theme	long-term	1664:1672	arg1	adaptation					1679:1688	long-term diet adaptation	1664:1688	long-term diet adaptation	1664:1688	Another more prominent trend was significant intercolony variation in digestibility across substrates, possibly resulting from differences in microbiota composition, long-term diet adaptation, or both.
26896534	3	82	theme	novel	464:468	arg1	approach					470:477	a novel approach	462:477	a novel approach for assessing digestibility of termite bait matrices and matrix components	462:552	This study took a novel approach for assessing digestibility of termite bait matrices and matrix components to gain potentially new insights into bait attractiveness and efficacy.
26896534	6	83	dep	controls	1251:1258	arg1	controls					1251:1258	positive controls	1242:1258	positive controls (southern yellow and northern pine)	1242:1294	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	83	dep	controls	1251:1258	arg1	yellow					1270:1275	southern yellow	1261:1275	southern yellow	1261:1275	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	6	83	dep	controls	1251:1258	arg1	pine					1290:1293	northern pine	1281:1293	northern pine	1281:1293	The substrates tested included two commercial bait matrices (Recruit IV and Recruit II HD), two matrix components (compressed and toasted compressed cellulose), and two natural pine woods as positive controls (southern yellow and northern pine).
26896534	4	84	theme	trophallactic	791:803	arg1	transfer					805:812	trophallactic transfer	791:812	trophallactic transfer of active ingredients through termite colonies	791:859	The rationale behind this study is that termite baits that are more digestible should have more nutritional value to termites and thus encourage maximal feeding and trophallactic transfer of active ingredients through termite colonies.
26896534	3	85	theme	termite	510:516	arg1	matrices					523:530	termite bait matrices	510:530	termite bait matrices	510:530	This study took a novel approach for assessing digestibility of termite bait matrices and matrix components to gain potentially new insights into bait attractiveness and efficacy.
25779605	2	0	theme	electron	519:526	arg1	microscope					528:537	transmission electron microscope	506:537	transmission electron microscope (TEM)	506:543	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	0	theme	electron	519:526	arg1	TEM					540:542	TEM	540:542	TEM	540:542	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	1	theme	vibration	598:606	arg1	magnetometer					615:626	vibration sample magnetometer	598:626	vibration sample magnetometer (VSM)	598:632	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	1	theme	vibration	598:606	arg1	VSM					629:631	VSM	629:631	VSM	629:631	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	2	theme	apoptosis	693:701	arg1	studies					703:709	apoptosis studies	693:709	apoptosis studies in vitro	693:718	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	3	from	cytotoxicity	662:673	arg1	vitro					714:718	vitro	714:718	vitro	714:718	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	4	theme	transmission	506:517	arg1	microscope					528:537	transmission electron microscope	506:537	transmission electron microscope (TEM)	506:543	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	4	theme	transmission	506:517	arg1	TEM					540:542	TEM	540:542	TEM	540:542	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	5	from	spectroscopy	484:495	arg1	vitro					714:718	vitro	714:718	vitro	714:718	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	6	theme	drug	639:642	arg1	kinetics					652:659	drug release kinetics	639:659	drug release kinetics	639:659	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	7	7	theme	Fe3O4/CMCS-Rapa	1524:1538	arg1	NPs					1540:1542	the Fe3O4/CMCS-Rapa NPs	1520:1542	the Fe3O4/CMCS-Rapa NPs	1520:1542	All these results demonstrated that the Fe3O4/CMCS-Rapa NPs may be useful as a promising candidate for targeted cancer diagnostic and therapy.
25779605	7	7	theme	Fe3O4/CMCS-Rapa	1524:1538	arg1	useful					1551:1556	useful	1551:1556	useful	1551:1556	All these results demonstrated that the Fe3O4/CMCS-Rapa NPs may be useful as a promising candidate for targeted cancer diagnostic and therapy.
25779605	4	8	theme	Fe3O4/CMCS	1018:1027	arg1	NPs					1029:1031	the Fe3O4/CMCS NPs	1014:1031	the Fe3O4/CMCS NPs	1014:1031	In addition, the in vitro drug release behavior displayed that the Fe3O4/CMCS NPs exhibited a biphasic drug release pattern with initial burst release and consequently sustained release.
25779605	3	9	theme	average	813:819	arg1	size					821:824	an average size	810:824	an average size of 30±2 nm	810:835	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	0	10	theme	in	122:123	arg1	nanoparticles					54:66	magnetic Fe3O4/carboxymethylchitosan nanoparticles	17:66	magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization	17:146	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	0	10	theme	in	122:123	arg1	characterization					131:146	in vitro characterization	122:146	in vitro characterization	122:146	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	6	11	theme	drug	1391:1394	arg1	damage					1396:1401	Rapa drug damage	1386:1401	Rapa drug damage to the normal cells so as to improve the curative effect of drug to tumor cells	1386:1481	Fe3O4/CMCS-Rapa NPs could enhance cellular uptake and reduce Rapa drug damage to the normal cells so as to improve the curative effect of drug to tumor cells.
25779605	6	12	theme	normal	1410:1415	arg1	cells					1417:1421	the normal cells	1406:1421	the normal cells so as to improve the curative effect of drug to tumor cells	1406:1481	Fe3O4/CMCS-Rapa NPs could enhance cellular uptake and reduce Rapa drug damage to the normal cells so as to improve the curative effect of drug to tumor cells.
25779605	5	13	theme	lower	1182:1186	arg1	cytotoxicity					1188:1199	lower cytotoxicity	1182:1199	lower cytotoxicity	1182:1199	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	5	14	theme	native	1312:1317	arg1	Rapa					1319:1322	native Rapa	1312:1322	native Rapa	1312:1322	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	4	15	theme	burst	1088:1092	arg1	release					1094:1100	initial burst release	1080:1100	initial burst release	1080:1100	In addition, the in vitro drug release behavior displayed that the Fe3O4/CMCS NPs exhibited a biphasic drug release pattern with initial burst release and consequently sustained release.
25779605	2	16	from	analysis	579:586	arg1	vitro					714:718	vitro	714:718	vitro	714:718	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	3	17	theme	synthesized	749:759	arg1	spherical					786:794	spherical	786:794	spherical	786:794	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	3	17	theme	synthesized	749:759	arg1	NPs					777:779	the synthesized Fe3O4/CMCS-Rapa NPs	745:779	the synthesized Fe3O4/CMCS-Rapa NPs	745:779	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	0	18	dep	in	122:123	arg1	vitro					125:129	vitro	125:129	vitro	125:129	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	4	19	theme	initial	1080:1086	arg1	release					1094:1100	initial burst release	1080:1100	initial burst release	1080:1100	In addition, the in vitro drug release behavior displayed that the Fe3O4/CMCS NPs exhibited a biphasic drug release pattern with initial burst release and consequently sustained release.
25779605	1	20	theme	Fe3O4/CMCS-Rapa	194:208	arg1	system					186:191	A novel tumor-targeted drug delivery system	149:191	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs)	149:213	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	20	theme	Fe3O4/CMCS-Rapa	194:208	arg1	NPs					210:212	Fe3O4/CMCS-Rapa NPs	194:212	Fe3O4/CMCS-Rapa NPs	194:212	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	4	21	theme	release	1059:1065	arg1	pattern					1067:1073	a biphasic drug release pattern	1043:1073	a biphasic drug release pattern	1043:1073	In addition, the in vitro drug release behavior displayed that the Fe3O4/CMCS NPs exhibited a biphasic drug release pattern with initial burst release and consequently sustained release.
25779605	2	22	dep	Fourier	457:463	arg1	transform					465:473	transform	465:473	transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro	465:718	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	5	23	theme	liver	1204:1208	arg1	LO2					1221:1223	LO2	1221:1223	LO2	1221:1223	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	5	23	theme	liver	1204:1208	arg1	line					1215:1218	liver cell line	1204:1218	liver cell line (LO2)	1204:1224	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	4	24	theme	drug	977:980	arg1	behavior					990:997	the in vitro drug release behavior	964:997	the in vitro drug release behavior	964:997	In addition, the in vitro drug release behavior displayed that the Fe3O4/CMCS NPs exhibited a biphasic drug release pattern with initial burst release and consequently sustained release.
25779605	5	25	theme	human	1267:1271	arg1	HepG2					1300:1304	HepG2	1300:1304	HepG2	1300:1304	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	5	25	theme	human	1267:1271	arg1	line					1294:1297	human hepatocarcinoma cell line	1267:1297	human hepatocarcinoma cell line (HepG2)	1267:1305	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	6	26	theme	tumor	1471:1475	arg1	cells					1477:1481	tumor cells	1471:1481	tumor cells	1471:1481	Fe3O4/CMCS-Rapa NPs could enhance cellular uptake and reduce Rapa drug damage to the normal cells so as to improve the curative effect of drug to tumor cells.
25779605	2	27	theme	infrared	475:482	arg1	FT-IR					498:502	FT-IR	498:502	FT-IR	498:502	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	27	theme	infrared	475:482	arg1	spectroscopy					484:495	infrared spectroscopy	475:495	infrared spectroscopy (FT-IR)	475:503	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	28	theme	sample	608:613	arg1	magnetometer					615:626	vibration sample magnetometer	598:626	vibration sample magnetometer (VSM)	598:632	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	28	theme	sample	608:613	arg1	VSM					629:631	VSM	629:631	VSM	629:631	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	4	29	theme	in	968:969	arg1	behavior					990:997	the in vitro drug release behavior	964:997	the in vitro drug release behavior	964:997	In addition, the in vitro drug release behavior displayed that the Fe3O4/CMCS NPs exhibited a biphasic drug release pattern with initial burst release and consequently sustained release.
25779605	3	30	theme	loading	894:900	arg1	%					948:948	approximately 6.32±0.34%	925:948	approximately 6.32±0.34%	925:948	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	3	30	theme	loading	894:900	arg1	efficiency					902:911	the loading efficiency	890:911	the loading efficiency of Rapa	890:919	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	2	31	from	uptake	685:690	arg1	vitro					714:718	vitro	714:718	vitro	714:718	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	0	32	theme	Fe3O4/carboxymethylchitosan	26:52	arg1	Synthesis					108:116	Synthesis	108:116	Synthesis	108:116	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	0	32	theme	Fe3O4/carboxymethylchitosan	26:52	arg1	nanoparticles					54:66	magnetic Fe3O4/carboxymethylchitosan nanoparticles	17:66	magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization	17:146	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	0	32	theme	Fe3O4/carboxymethylchitosan	26:52	arg1	characterization					131:146	in vitro characterization	122:146	in vitro characterization	122:146	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	7	33	theme	targeted	1587:1594	arg1	cancer					1596:1601	targeted cancer diagnostic	1587:1612	targeted cancer diagnostic	1587:1612	All these results demonstrated that the Fe3O4/CMCS-Rapa NPs may be useful as a promising candidate for targeted cancer diagnostic and therapy.
25779605	3	34	from	shape	799:803	arg1	spherical					786:794	spherical	786:794	spherical	786:794	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	3	34	from	shape	799:803	arg1	NPs					777:779	the synthesized Fe3O4/CMCS-Rapa NPs	745:779	the synthesized Fe3O4/CMCS-Rapa NPs	745:779	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	2	35	theme	release	644:650	arg1	kinetics					652:659	drug release kinetics	639:659	drug release kinetics	639:659	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	4	36	theme	drug	1054:1057	arg1	pattern					1067:1073	a biphasic drug release pattern	1043:1073	a biphasic drug release pattern	1043:1073	In addition, the in vitro drug release behavior displayed that the Fe3O4/CMCS NPs exhibited a biphasic drug release pattern with initial burst release and consequently sustained release.
25779605	2	37	from	studies	703:709	arg1	vitro					714:718	vitro	714:718	vitro	714:718	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	0	38	theme	magnetic	17:24	arg1	Synthesis					108:116	Synthesis	108:116	Synthesis	108:116	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	0	38	theme	magnetic	17:24	arg1	nanoparticles					54:66	magnetic Fe3O4/carboxymethylchitosan nanoparticles	17:66	magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization	17:146	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	0	38	theme	magnetic	17:24	arg1	characterization					131:146	in vitro characterization	122:146	in vitro characterization	122:146	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	7	39	theme	promising	1563:1571	arg1	candidate					1573:1581	a promising candidate	1561:1581	a promising candidate for targeted cancer diagnostic and therapy	1561:1624	All these results demonstrated that the Fe3O4/CMCS-Rapa NPs may be useful as a promising candidate for targeted cancer diagnostic and therapy.
25779605	5	40	theme	cell	1289:1292	arg1	HepG2					1300:1304	HepG2	1300:1304	HepG2	1300:1304	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	5	40	theme	cell	1289:1292	arg1	line					1294:1297	human hepatocarcinoma cell line	1267:1297	human hepatocarcinoma cell line (HepG2)	1267:1305	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	2	41	from	kinetics	652:659	arg1	vitro					714:718	vitro	714:718	vitro	714:718	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	6	42	theme	Rapa	1386:1389	arg1	damage					1396:1401	Rapa drug damage	1386:1401	Rapa drug damage to the normal cells so as to improve the curative effect of drug to tumor cells	1386:1481	Fe3O4/CMCS-Rapa NPs could enhance cellular uptake and reduce Rapa drug damage to the normal cells so as to improve the curative effect of drug to tumor cells.
25779605	5	43	theme	Fe3O4/CMCS-Rapa	1155:1169	arg1	NPs					1171:1173	the Fe3O4/CMCS-Rapa NPs	1151:1173	the Fe3O4/CMCS-Rapa NPs	1151:1173	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	7	44	theme	diagnostic	1603:1612	arg1	cancer					1596:1601	targeted cancer diagnostic	1587:1612	targeted cancer diagnostic	1587:1612	All these results demonstrated that the Fe3O4/CMCS-Rapa NPs may be useful as a promising candidate for targeted cancer diagnostic and therapy.
25779605	1	45	theme	magnetic	234:241	arg1	nanoparticles					271:283	magnetic Fe3O4/carboxymethylchitosan nanoparticles	234:283	magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa)	234:332	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	45	theme	magnetic	234:241	arg1	NPs					297:299	Fe3O4/CMCS NPs	286:299	Fe3O4/CMCS NPs	286:299	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	45	theme	magnetic	234:241	arg1	drug					358:361	the model anti-tumor drug	337:361	the model anti-tumor drug	337:361	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	2	46	theme	Fe3O4/CMCS-Rapa	415:429	arg1	NPs					431:433	the Fe3O4/CMCS-Rapa NPs	411:433	the Fe3O4/CMCS-Rapa NPs	411:433	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	47	from	magnetometer	615:626	arg1	vitro					714:718	vitro	714:718	vitro	714:718	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	5	48	theme	cell	1210:1213	arg1	LO2					1221:1223	LO2	1221:1223	LO2	1221:1223	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	5	48	theme	cell	1210:1213	arg1	line					1215:1218	liver cell line	1204:1218	liver cell line (LO2)	1204:1224	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	1	49	theme	Fe3O4/carboxymethylchitosan	243:269	arg1	nanoparticles					271:283	magnetic Fe3O4/carboxymethylchitosan nanoparticles	234:283	magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa)	234:332	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	49	theme	Fe3O4/carboxymethylchitosan	243:269	arg1	NPs					297:299	Fe3O4/CMCS NPs	286:299	Fe3O4/CMCS NPs	286:299	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	49	theme	Fe3O4/carboxymethylchitosan	243:269	arg1	drug					358:361	the model anti-tumor drug	337:361	the model anti-tumor drug	337:361	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	0	50	theme	drug	86:89	arg1	system					100:105	tumor-targeted drug delivery system	71:105	tumor-targeted drug delivery system	71:105	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	2	51	theme	X-ray	546:550	arg1	XRD					565:567	XRD	565:567	XRD	565:567	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	51	theme	X-ray	546:550	arg1	diffraction					552:562	X-ray diffraction	546:562	X-ray diffraction (XRD)	546:568	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	6	52	theme	Fe3O4/CMCS-Rapa	1325:1339	arg1	NPs					1341:1343	Fe3O4/CMCS-Rapa NPs	1325:1343	Fe3O4/CMCS-Rapa NPs	1325:1343	Fe3O4/CMCS-Rapa NPs could enhance cellular uptake and reduce Rapa drug damage to the normal cells so as to improve the curative effect of drug to tumor cells.
25779605	2	53	from	microscope	528:537	arg1	vitro					714:718	vitro	714:718	vitro	714:718	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	3	54	from	spherical	786:794	arg1	shape					799:803	shape	799:803	shape	799:803	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	0	55	theme	tumor-targeted	71:84	arg1	system					100:105	tumor-targeted drug delivery system	71:105	tumor-targeted drug delivery system	71:105	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	1	56	theme	model	341:345	arg1	drug					358:361	the model anti-tumor drug	337:361	the model anti-tumor drug	337:361	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	56	theme	model	341:345	arg1	nanoparticles					271:283	magnetic Fe3O4/carboxymethylchitosan nanoparticles	234:283	magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa)	234:332	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	0	57	dep	nanoparticles	54:66	arg1	Synthesis					108:116	Synthesis	108:116	Synthesis	108:116	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	0	57	dep	nanoparticles	54:66	arg1	nanoparticles					54:66	magnetic Fe3O4/carboxymethylchitosan nanoparticles	17:66	magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization	17:146	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	0	57	dep	nanoparticles	54:66	arg1	characterization					131:146	in vitro characterization	122:146	in vitro characterization	122:146	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	2	58	theme	cellular	676:683	arg1	uptake					685:690	cellular uptake	676:690	cellular uptake	676:690	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	5	59	theme	hepatocarcinoma	1273:1287	arg1	HepG2					1300:1304	HepG2	1300:1304	HepG2	1300:1304	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	5	59	theme	hepatocarcinoma	1273:1287	arg1	line					1294:1297	human hepatocarcinoma cell line	1267:1297	human hepatocarcinoma cell line (HepG2)	1267:1305	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	6	60	theme	curative	1444:1451	arg1	effect					1453:1458	the curative effect	1440:1458	the curative effect of drug to tumor cells	1440:1481	Fe3O4/CMCS-Rapa NPs could enhance cellular uptake and reduce Rapa drug damage to the normal cells so as to improve the curative effect of drug to tumor cells.
25779605	1	61	theme	anti-tumor	347:356	arg1	drug					358:361	the model anti-tumor drug	337:361	the model anti-tumor drug	337:361	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	61	theme	anti-tumor	347:356	arg1	nanoparticles					271:283	magnetic Fe3O4/carboxymethylchitosan nanoparticles	234:283	magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa)	234:332	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	2	62	theme	thermal	571:577	arg1	TG/DSC					589:594	TG/DSC	589:594	TG/DSC	589:594	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	62	theme	thermal	571:577	arg1	analysis					579:586	thermal analysis	571:586	thermal analysis (TG/DSC)	571:595	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	6	63	theme	drug	1463:1466	arg1	effect					1453:1458	the curative effect	1440:1458	the curative effect of drug to tumor cells	1440:1481	Fe3O4/CMCS-Rapa NPs could enhance cellular uptake and reduce Rapa drug damage to the normal cells so as to improve the curative effect of drug to tumor cells.
25779605	3	64	theme	Rapa	916:919	arg1	%					948:948	approximately 6.32±0.34%	925:948	approximately 6.32±0.34%	925:948	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	3	64	theme	Rapa	916:919	arg1	efficiency					902:911	the loading efficiency	890:911	the loading efficiency of Rapa	890:919	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	1	65	theme	novel	151:155	arg1	system					186:191	A novel tumor-targeted drug delivery system	149:191	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs)	149:213	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	65	theme	novel	151:155	arg1	NPs					210:212	Fe3O4/CMCS-Rapa NPs	194:212	Fe3O4/CMCS-Rapa NPs	194:212	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	3	66	theme	nm	834:835	arg1	size					821:824	an average size	810:824	an average size of 30±2 nm	810:835	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	4	67	dep	in	968:969	arg1	vitro					971:975	vitro	971:975	vitro	971:975	In addition, the in vitro drug release behavior displayed that the Fe3O4/CMCS NPs exhibited a biphasic drug release pattern with initial burst release and consequently sustained release.
25779605	4	68	theme	sustained	1119:1127	arg1	release					1129:1135	consequently sustained release	1106:1135	consequently sustained release	1106:1135	In addition, the in vitro drug release behavior displayed that the Fe3O4/CMCS NPs exhibited a biphasic drug release pattern with initial burst release and consequently sustained release.
25779605	1	69	theme	tumor-targeted	157:170	arg1	system					186:191	A novel tumor-targeted drug delivery system	149:191	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs)	149:213	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	69	theme	tumor-targeted	157:170	arg1	NPs					210:212	Fe3O4/CMCS-Rapa NPs	194:212	Fe3O4/CMCS-Rapa NPs	194:212	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	4	70	theme	biphasic	1045:1052	arg1	pattern					1067:1073	a biphasic drug release pattern	1043:1073	a biphasic drug release pattern	1043:1073	In addition, the in vitro drug release behavior displayed that the Fe3O4/CMCS NPs exhibited a biphasic drug release pattern with initial burst release and consequently sustained release.
25779605	2	71	from	diffraction	552:562	arg1	vitro					714:718	vitro	714:718	vitro	714:718	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	6	72	theme	cellular	1359:1366	arg1	uptake					1368:1373	cellular uptake	1359:1373	cellular uptake	1359:1373	Fe3O4/CMCS-Rapa NPs could enhance cellular uptake and reduce Rapa drug damage to the normal cells so as to improve the curative effect of drug to tumor cells.
25779605	2	73	theme	NPs	431:433	arg1	composition					380:390	composition	380:390	composition	380:390	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	73	theme	NPs	431:433	arg1	properties					397:406	properties	397:406	properties of the Fe3O4/CMCS-Rapa NPs	397:433	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	2	73	theme	NPs	431:433	arg1	morphology					368:377	The morphology	364:377	The morphology	364:377	The morphology, composition, and properties of the Fe3O4/CMCS-Rapa NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD), thermal analysis (TG/DSC), vibration sample magnetometer (VSM), and drug release kinetics, cytotoxicity, cellular uptake, apoptosis studies in vitro.
25779605	1	74	theme	drug	172:175	arg1	system					186:191	A novel tumor-targeted drug delivery system	149:191	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs)	149:213	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	74	theme	drug	172:175	arg1	NPs					210:212	Fe3O4/CMCS-Rapa NPs	194:212	Fe3O4/CMCS-Rapa NPs	194:212	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	5	75	theme	higher	1244:1249	arg1	cytotoxicity					1251:1262	comparatively higher cytotoxicity	1230:1262	comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2)	1230:1305	Furthermore, the Fe3O4/CMCS-Rapa NPs showed lower cytotoxicity to liver cell line (LO2) and comparatively higher cytotoxicity to human hepatocarcinoma cell line (HepG2) than native Rapa.
25779605	1	76	theme	Fe3O4/CMCS	286:295	arg1	nanoparticles					271:283	magnetic Fe3O4/carboxymethylchitosan nanoparticles	234:283	magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa)	234:332	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	76	theme	Fe3O4/CMCS	286:295	arg1	NPs					297:299	Fe3O4/CMCS NPs	286:299	Fe3O4/CMCS NPs	286:299	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	0	77	theme	delivery	91:98	arg1	system					100:105	tumor-targeted drug delivery system	71:105	tumor-targeted drug delivery system	71:105	Rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles as tumor-targeted drug delivery system: Synthesis and in vitro characterization.
25779605	4	78	theme	release	982:988	arg1	behavior					990:997	the in vitro drug release behavior	964:997	the in vitro drug release behavior	964:997	In addition, the in vitro drug release behavior displayed that the Fe3O4/CMCS NPs exhibited a biphasic drug release pattern with initial burst release and consequently sustained release.
25779605	3	79	with	spherical	786:794	arg1	size					821:824	an average size	810:824	an average size of 30±2 nm	810:835	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	1	80	theme	delivery	177:184	arg1	system					186:191	A novel tumor-targeted drug delivery system	149:191	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs)	149:213	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	1	80	theme	delivery	177:184	arg1	NPs					210:212	Fe3O4/CMCS-Rapa NPs	194:212	Fe3O4/CMCS-Rapa NPs	194:212	A novel tumor-targeted drug delivery system (Fe3O4/CMCS-Rapa NPs) was prepared using magnetic Fe3O4/carboxymethylchitosan nanoparticles (Fe3O4/CMCS NPs) as carrier and rapamycin (Rapa) as the model anti-tumor drug.
25779605	3	81	theme	saturated	842:850	arg1	magnetization					852:864	the saturated magnetization	838:864	the saturated magnetization	838:864	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	3	82	theme	Fe3O4/CMCS-Rapa	761:775	arg1	spherical					786:794	spherical	786:794	spherical	786:794	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
25779605	3	82	theme	Fe3O4/CMCS-Rapa	761:775	arg1	NPs					777:779	the synthesized Fe3O4/CMCS-Rapa NPs	745:779	the synthesized Fe3O4/CMCS-Rapa NPs	745:779	The results showed that the synthesized Fe3O4/CMCS-Rapa NPs were spherical in shape with an average size of 30±2 nm, the saturated magnetization reached 67.1 emu/g, and the loading efficiency of Rapa was approximately 6.32±0.34%.
29050607	5	0	theme	novel	915:919	arg1	coating					926:932	the novel food coating	911:932	the novel food coating	911:932	The antimicrobial capacity of the novel food coating was tested on strawberries, a fruit with high perishability.
29050607	0	1	theme	olive	79:83	arg1	phenols					85:91	the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol	75:136	the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol	75:136	Physical and functional properties of pectin-fish gelatin films containing the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol.
29050607	0	1	theme	olive	79:83	arg1	3,4-dihydroxyphenylglycol					112:136	3,4-dihydroxyphenylglycol	112:136	3,4-dihydroxyphenylglycol	112:136	Physical and functional properties of pectin-fish gelatin films containing the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol.
29050607	0	1	theme	olive	79:83	arg1	hydroxytyrosol					93:106	hydroxytyrosol	93:106	hydroxytyrosol	93:106	Physical and functional properties of pectin-fish gelatin films containing the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol.
29050607	3	2	theme	vapor	694:698	arg1	WVP					714:716	WVP	714:716	WVP	714:716	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	3	2	theme	vapor	694:698	arg1	permeability					700:711	water vapor permeability	688:711	water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa	688:805	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	1	3	theme	composite	181:189	arg1	film					198:201	a composite edible film	179:201	a composite edible film based on pectin and fish skin protein capable of protecting food from microbial attack and oxidative degradation	179:314	This study describes the development of a composite edible film based on pectin and fish skin protein capable of protecting food from microbial attack and oxidative degradation.
29050607	6	4	theme	edible	1009:1014	arg1	film					1016:1019	The bioactive edible film	995:1019	The bioactive edible film containing HT/DHPG	995:1038	The bioactive edible film containing HT/DHPG preserved the strawberries against mold during storage with a significant delay in visible decay.
29050607	3	5	theme	permeability	700:711	arg1	values					678:683	values	678:683	values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa	678:805	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	3	6	theme	concentration	550:562	arg1	influence					533:541	The influence	529:541	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films	529:653	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	6	7	from	delay	1114:1118	arg1	decay					1131:1135	visible decay	1123:1135	visible decay	1123:1135	The bioactive edible film containing HT/DHPG preserved the strawberries against mold during storage with a significant delay in visible decay.
29050607	1	8	theme	microbial	273:281	arg1	attack					283:288	microbial attack	273:288	microbial attack	273:288	This study describes the development of a composite edible film based on pectin and fish skin protein capable of protecting food from microbial attack and oxidative degradation.
29050607	3	9	theme	active	583:588	arg1	HT/DHPG					590:596	active HT/DHPG	583:596	active HT/DHPG	583:596	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	5	10	theme	high	975:978	arg1	perishability					980:992	high perishability	975:992	high perishability	975:992	The antimicrobial capacity of the novel food coating was tested on strawberries, a fruit with high perishability.
29050607	3	11	from	influence	533:541	arg1	properties					631:640	the mechanical and functional properties	601:640	the mechanical and functional properties of the films	601:653	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	5	12	theme	antimicrobial	885:897	arg1	capacity					899:906	The antimicrobial capacity	881:906	The antimicrobial capacity of the novel food coating	881:932	The antimicrobial capacity of the novel food coating was tested on strawberries, a fruit with high perishability.
29050607	1	13	theme	edible	191:196	arg1	film					198:201	a composite edible film	179:201	a composite edible film based on pectin and fish skin protein capable of protecting food from microbial attack and oxidative degradation	179:314	This study describes the development of a composite edible film based on pectin and fish skin protein capable of protecting food from microbial attack and oxidative degradation.
29050607	0	14	theme	pectin-fish	38:48	arg1	films					58:62	pectin-fish gelatin films	38:62	pectin-fish gelatin films	38:62	Physical and functional properties of pectin-fish gelatin films containing the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol.
29050607	5	15	with	fruit	964:968	arg1	perishability					980:992	high perishability	975:992	high perishability	975:992	The antimicrobial capacity of the novel food coating was tested on strawberries, a fruit with high perishability.
29050607	1	16	theme	film	198:201	arg1	development					164:174	the development	160:174	the development of a composite edible film based on pectin and fish skin protein capable of protecting food from microbial attack and oxidative degradation	160:314	This study describes the development of a composite edible film based on pectin and fish skin protein capable of protecting food from microbial attack and oxidative degradation.
29050607	4	17	from	values	854:859	arg1	behavior					820:827	The release behavior	808:827	The release behavior in water at different pH values	808:859	The release behavior in water at different pH values was also evaluated.
29050607	0	18	dep	phenols	85:91	arg1	phenols					85:91	the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol	75:136	the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol	75:136	Physical and functional properties of pectin-fish gelatin films containing the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol.
29050607	0	18	dep	phenols	85:91	arg1	3,4-dihydroxyphenylglycol					112:136	3,4-dihydroxyphenylglycol	112:136	3,4-dihydroxyphenylglycol	112:136	Physical and functional properties of pectin-fish gelatin films containing the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol.
29050607	0	18	dep	phenols	85:91	arg1	hydroxytyrosol					93:106	hydroxytyrosol	93:106	hydroxytyrosol	93:106	Physical and functional properties of pectin-fish gelatin films containing the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol.
29050607	2	19	theme	phenolic	406:413	arg1	compounds					415:423	the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit,	372:509	compounds	415:423	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	2	19	theme	phenolic	406:413	arg1	3,4-dihydroxyphenylglycol					449:473	3,4-dihydroxyphenylglycol	449:473	3,4-dihydroxyphenylglycol (DHPG)	449:480	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	2	19	theme	phenolic	406:413	arg1	hydroxytyrosol					425:438	hydroxytyrosol	425:438	hydroxytyrosol (HT)	425:443	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	0	20	theme	Physical	0:7	arg1	properties					24:33	Physical and functional properties	0:33	Physical and functional properties of pectin-fish gelatin films	0:62	Physical and functional properties of pectin-fish gelatin films containing the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol.
29050607	3	21	theme	HT/DHPG	590:596	arg1	concentration					550:562	the concentration	546:562	the concentration of plasticizer and active HT/DHPG	546:596	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	1	22	theme	oxidative	294:302	arg1	degradation					304:314	oxidative degradation	294:314	oxidative degradation	294:314	This study describes the development of a composite edible film based on pectin and fish skin protein capable of protecting food from microbial attack and oxidative degradation.
29050607	3	23	theme	plasticizer	567:577	arg1	concentration					550:562	the concentration	546:562	the concentration of plasticizer and active HT/DHPG	546:596	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	0	24	theme	functional	13:22	arg1	properties					24:33	Physical and functional properties	0:33	Physical and functional properties of pectin-fish gelatin films	0:62	Physical and functional properties of pectin-fish gelatin films containing the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol.
29050607	3	25	theme	oxygen	751:756	arg1	permeability					758:769	oxygen permeability	751:769	oxygen permeability	751:769	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	5	26	theme	food	921:924	arg1	coating					926:932	the novel food coating	911:932	the novel food coating	911:932	The antimicrobial capacity of the novel food coating was tested on strawberries, a fruit with high perishability.
29050607	2	27	theme	olive	498:502	arg1	fruit					504:508	olive fruit	498:508	olive fruit	498:508	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	3	28	theme	mechanical	605:614	arg1	properties					631:640	the mechanical and functional properties	601:640	the mechanical and functional properties of the films	601:653	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	1	29	theme	fish	223:226	arg1	protein					233:239	fish skin protein	223:239	fish skin protein capable of protecting food from microbial attack and oxidative degradation	223:314	This study describes the development of a composite edible film based on pectin and fish skin protein capable of protecting food from microbial attack and oxidative degradation.
29050607	6	30	theme	significant	1102:1112	arg1	delay					1114:1118	a significant delay	1100:1118	a significant delay in visible decay	1100:1135	The bioactive edible film containing HT/DHPG preserved the strawberries against mold during storage with a significant delay in visible decay.
29050607	3	31	theme	films	649:653	arg1	properties					631:640	the mechanical and functional properties	601:640	the mechanical and functional properties of the films	601:653	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	1	32	theme	skin	228:231	arg1	protein					233:239	fish skin protein	223:239	fish skin protein capable of protecting food from microbial attack and oxidative degradation	223:314	This study describes the development of a composite edible film based on pectin and fish skin protein capable of protecting food from microbial attack and oxidative degradation.
29050607	0	33	theme	films	58:62	arg1	properties					24:33	Physical and functional properties	0:33	Physical and functional properties of pectin-fish gelatin films	0:62	Physical and functional properties of pectin-fish gelatin films containing the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol.
29050607	4	34	theme	pH	851:852	arg1	values					854:859	different pH values	841:859	different pH values	841:859	The release behavior in water at different pH values was also evaluated.
29050607	5	35	theme	coating	926:932	arg1	capacity					899:906	The antimicrobial capacity	881:906	The antimicrobial capacity of the novel food coating	881:932	The antimicrobial capacity of the novel food coating was tested on strawberries, a fruit with high perishability.
29050607	0	36	theme	gelatin	50:56	arg1	films					58:62	pectin-fish gelatin films	38:62	pectin-fish gelatin films	38:62	Physical and functional properties of pectin-fish gelatin films containing the olive phenols hydroxytyrosol and 3,4-dihydroxyphenylglycol.
29050607	4	37	theme	different	841:849	arg1	values					854:859	different pH values	841:859	different pH values	841:859	The release behavior in water at different pH values was also evaluated.
29050607	3	38	theme	water	688:692	arg1	WVP					714:716	WVP	714:716	WVP	714:716	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	3	38	theme	water	688:692	arg1	permeability					700:711	water vapor permeability	688:711	water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa	688:805	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	4	39	theme	release	812:818	arg1	behavior					820:827	The release behavior	808:827	The release behavior in water at different pH values	808:859	The release behavior in water at different pH values was also evaluated.
29050607	6	40	contain	containing	1021:1030	arg2	HT/DHPG					1032:1038	HT/DHPG	1032:1038	HT/DHPG	1032:1038	The bioactive edible film containing HT/DHPG preserved the strawberries against mold during storage with a significant delay in visible decay.
29050607	6	40	contain	containing	1021:1030	arg1	film					1016:1019	The bioactive edible film	995:1019	The bioactive edible film containing HT/DHPG	995:1038	The bioactive edible film containing HT/DHPG preserved the strawberries against mold during storage with a significant delay in visible decay.
29050607	6	41	theme	visible	1123:1129	arg1	decay					1131:1135	visible decay	1123:1135	visible decay	1123:1135	The bioactive edible film containing HT/DHPG preserved the strawberries against mold during storage with a significant delay in visible decay.
29050607	1	42	theme	capable	241:247	arg1	protein					233:239	fish skin protein	223:239	fish skin protein capable of protecting food from microbial attack and oxidative degradation	223:314	This study describes the development of a composite edible film based on pectin and fish skin protein capable of protecting food from microbial attack and oxidative degradation.
29050607	2	43	theme	active	514:519	arg1	agents					521:526	active agents	514:526	active agents	514:526	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	3	44	theme	functional	620:629	arg1	properties					631:640	the mechanical and functional properties	601:640	the mechanical and functional properties of the films	601:653	The influence of the concentration of plasticizer and active HT/DHPG on the mechanical and functional properties of the films was investigated, with values of water vapor permeability (WVP) between 0.13-0.22gmm/hm2kPa and oxygen permeability (OP) between 9.91-40.76cm3μm/m2dkPa.
29050607	4	45	from	behavior	820:827	arg1	water					832:836	water	832:836	water	832:836	The release behavior in water at different pH values was also evaluated.
29050607	6	46	theme	bioactive	999:1007	arg1	film					1016:1019	The bioactive edible film	995:1019	The bioactive edible film containing HT/DHPG	995:1038	The bioactive edible film containing HT/DHPG preserved the strawberries against mold during storage with a significant delay in visible decay.
29050607	2	47	dep	compounds	415:423	arg1	DHPG					476:479	DHPG	476:479	DHPG	476:479	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	2	47	dep	compounds	415:423	arg1	HT					441:442	HT	441:442	HT	441:442	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	2	47	dep	compounds	415:423	arg1	3,4-dihydroxyphenylglycol					449:473	3,4-dihydroxyphenylglycol	449:473	3,4-dihydroxyphenylglycol (DHPG)	449:480	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	2	47	dep	compounds	415:423	arg1	hydroxytyrosol					425:438	hydroxytyrosol	425:438	hydroxytyrosol (HT)	425:443	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	2	47	dep	compounds	415:423	arg1	compounds					415:423	the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit,	372:509	compounds	415:423	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	2	48	theme	antioxidant	376:386	arg1	compounds					415:423	the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit,	372:509	compounds	415:423	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	2	48	theme	antioxidant	376:386	arg1	3,4-dihydroxyphenylglycol					449:473	3,4-dihydroxyphenylglycol	449:473	3,4-dihydroxyphenylglycol (DHPG)	449:480	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
29050607	2	48	theme	antioxidant	376:386	arg1	hydroxytyrosol					425:438	hydroxytyrosol	425:438	hydroxytyrosol (HT)	425:443	The film was prepared with glycerol as plasticizer and the antioxidant and antimicrobial phenolic compounds hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), extracted from olive fruit, as active agents.
25491838	5	0	theme	high	758:761	arg1	temperature					784:794	a high thermal denaturation temperature	756:794	a high thermal denaturation temperature of the scaffold (200-250°C)	756:822	Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) results revealed a high thermal denaturation temperature of the scaffold (200-250°C).
25491838	6	1	theme	uptake	934:939	arg1	capacity					941:948	water uptake capacity	928:948	water uptake capacity (>1700%)	928:957	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	6	1	theme	uptake	934:939	arg1	%					956:956	>1700%	951:956	>1700%	951:956	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	7	2	theme	composite	1059:1067	arg1	scaffold					1069:1076	the keratin composite scaffold	1047:1076	the keratin composite scaffold observed using a Scanning Electron Microscope (SEM)	1047:1128	The morphology of the keratin composite scaffold observed using a Scanning Electron Microscope (SEM) exhibited good porosity and interconnectivity of pores.
25491838	8	3	theme	keratin	1271:1277	arg1	scaffold					1289:1296	the keratin composite scaffold	1267:1296	the keratin composite scaffold	1267:1296	MTT assay using NIH 3T3 fibroblast cells demonstrated that the cell viability of the keratin composite scaffold was good.
25491838	4	4	theme	biopolymers	595:605	arg1	structure					571:579	the native structure	560:579	the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold	560:659	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	5	5	theme	thermal	763:769	arg1	temperature					784:794	a high thermal denaturation temperature	756:794	a high thermal denaturation temperature of the scaffold (200-250°C)	756:822	Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) results revealed a high thermal denaturation temperature of the scaffold (200-250°C).
25491838	2	6	contain	containing	339:348	arg2	gelatin					372:378	gelatin	372:378	gelatin	372:378	In this paper, a novel biomimetic scaffold containing keratin, chitosan and gelatin was prepared by freeze drying method.
25491838	2	6	contain	containing	339:348	arg2	chitosan					359:366	chitosan	359:366	chitosan	359:366	In this paper, a novel biomimetic scaffold containing keratin, chitosan and gelatin was prepared by freeze drying method.
25491838	2	6	contain	containing	339:348	arg2	keratin					350:356	keratin	350:356	keratin	350:356	In this paper, a novel biomimetic scaffold containing keratin, chitosan and gelatin was prepared by freeze drying method.
25491838	2	6	contain	containing	339:348	arg1	scaffold					330:337	a novel biomimetic scaffold	311:337	a novel biomimetic scaffold containing keratin, chitosan and gelatin	311:378	In this paper, a novel biomimetic scaffold containing keratin, chitosan and gelatin was prepared by freeze drying method.
25491838	7	7	theme	scaffold	1069:1076	arg1	morphology					1033:1042	The morphology	1029:1042	The morphology of the keratin composite scaffold observed using a Scanning Electron Microscope (SEM)	1029:1128	The morphology of the keratin composite scaffold observed using a Scanning Electron Microscope (SEM) exhibited good porosity and interconnectivity of pores.
25491838	1	8	theme	wound	162:166	arg1	healing					168:174	wound healing	162:174	wound healing owing to its biocompatibility, biodegradability, intrinsic biological activity and presence of cellular binding motifs	162:293	Keratin has gained much attention in the recent past as a biomaterial for wound healing owing to its biocompatibility, biodegradability, intrinsic biological activity and presence of cellular binding motifs.
25491838	4	9	theme	individual	584:593	arg1	chitosan					617:624	chitosan	617:624	chitosan	617:624	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	4	9	theme	individual	584:593	arg1	gelatin					631:637	gelatin	631:637	gelatin	631:637	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	4	9	theme	individual	584:593	arg1	keratin					608:614	keratin	608:614	keratin	608:614	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	4	9	theme	individual	584:593	arg1	biopolymers					595:605	individual biopolymers	584:605	individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold	584:659	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	7	10	theme	good	1140:1143	arg1	porosity					1145:1152	good porosity	1140:1152	good porosity	1140:1152	The morphology of the keratin composite scaffold observed using a Scanning Electron Microscope (SEM) exhibited good porosity and interconnectivity of pores.
25491838	6	11	used	used	1012:1015	arg2	control					1020:1026	control	1020:1026	control	1020:1026	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	6	11	used	used	1012:1015	arg2	which					1002:1006	which	1002:1006	which	1002:1006	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	8	12	theme	scaffold	1289:1296	arg1	good					1302:1305	good	1302:1305	good	1302:1305	MTT assay using NIH 3T3 fibroblast cells demonstrated that the cell viability of the keratin composite scaffold was good.
25491838	8	12	theme	scaffold	1289:1296	arg1	viability					1254:1262	the cell viability	1245:1262	the cell viability of the keratin composite scaffold	1245:1296	MTT assay using NIH 3T3 fibroblast cells demonstrated that the cell viability of the keratin composite scaffold was good.
25491838	1	13	theme	cellular	271:278	arg1	motifs					288:293	cellular binding motifs	271:293	cellular binding motifs	271:293	Keratin has gained much attention in the recent past as a biomaterial for wound healing owing to its biocompatibility, biodegradability, intrinsic biological activity and presence of cellular binding motifs.
25491838	5	14	theme	Thermogravimetric	662:678	arg1	TGA					690:692	TGA	690:692	TGA	690:692	Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) results revealed a high thermal denaturation temperature of the scaffold (200-250°C).
25491838	5	14	theme	Thermogravimetric	662:678	arg1	Analysis					680:687	Thermogravimetric Analysis	662:687	Thermogravimetric Analysis (TGA)	662:693	Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) results revealed a high thermal denaturation temperature of the scaffold (200-250°C).
25491838	8	15	theme	composite	1279:1287	arg1	scaffold					1289:1296	the keratin composite scaffold	1267:1296	the keratin composite scaffold	1267:1296	MTT assay using NIH 3T3 fibroblast cells demonstrated that the cell viability of the keratin composite scaffold was good.
25491838	1	16	theme	binding	280:286	arg1	motifs					288:293	cellular binding motifs	271:293	cellular binding motifs	271:293	Keratin has gained much attention in the recent past as a biomaterial for wound healing owing to its biocompatibility, biodegradability, intrinsic biological activity and presence of cellular binding motifs.
25491838	5	17	dep	Analysis	680:687	arg1	results					739:745	results	739:745	results	739:745	Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) results revealed a high thermal denaturation temperature of the scaffold (200-250°C).
25491838	9	18	theme	promising	1393:1401	arg1	alternative					1403:1413	a promising alternative	1391:1413	a promising alternative biomaterial for tissue engineering applications	1391:1461	These observations suggest that the keratin-chitosan-gelatin composite scaffold is a promising alternative biomaterial for tissue engineering applications.
25491838	9	18	theme	promising	1393:1401	arg1	scaffold					1379:1386	the keratin-chitosan-gelatin composite scaffold	1340:1386	the keratin-chitosan-gelatin composite scaffold	1340:1386	These observations suggest that the keratin-chitosan-gelatin composite scaffold is a promising alternative biomaterial for tissue engineering applications.
25491838	2	19	theme	freeze	396:401	arg1	method					410:415	freeze drying method	396:415	freeze drying method	396:415	In this paper, a novel biomimetic scaffold containing keratin, chitosan and gelatin was prepared by freeze drying method.
25491838	9	20	theme	engineering	1438:1448	arg1	applications					1450:1461	tissue engineering applications	1431:1461	tissue engineering applications	1431:1461	These observations suggest that the keratin-chitosan-gelatin composite scaffold is a promising alternative biomaterial for tissue engineering applications.
25491838	4	21	theme	Infrared	507:514	arg1	spectroscopy					523:534	Infrared (FTIR) spectroscopy	507:534	Infrared (FTIR) spectroscopy	507:534	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	6	22	theme	comparable	959:968	arg1	kPa					887:889	96 kPa	884:889	96 kPa	884:889	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	6	22	theme	comparable	959:968	arg1	strength					874:881	tensile strength	866:881	tensile strength (96 kPa)	866:890	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	9	23	theme	biomaterial	1415:1425	arg1	alternative					1403:1413	a promising alternative	1391:1413	a promising alternative biomaterial for tissue engineering applications	1391:1461	These observations suggest that the keratin-chitosan-gelatin composite scaffold is a promising alternative biomaterial for tissue engineering applications.
25491838	9	23	theme	biomaterial	1415:1425	arg1	scaffold					1379:1386	the keratin-chitosan-gelatin composite scaffold	1340:1386	the keratin-chitosan-gelatin composite scaffold	1340:1386	These observations suggest that the keratin-chitosan-gelatin composite scaffold is a promising alternative biomaterial for tissue engineering applications.
25491838	5	24	theme	scaffold	803:810	arg1	temperature					784:794	a high thermal denaturation temperature	756:794	a high thermal denaturation temperature of the scaffold (200-250°C)	756:822	Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) results revealed a high thermal denaturation temperature of the scaffold (200-250°C).
25491838	6	25	theme	water	928:932	arg1	capacity					941:948	water uptake capacity	928:948	water uptake capacity (>1700%)	928:957	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	6	25	theme	water	928:932	arg1	%					956:956	>1700%	951:956	>1700%	951:956	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	4	26	theme	native	564:569	arg1	structure					571:579	the native structure	560:579	the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold	560:659	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	2	27	theme	novel	313:317	arg1	scaffold					330:337	a novel biomimetic scaffold	311:337	a novel biomimetic scaffold containing keratin, chitosan and gelatin	311:378	In this paper, a novel biomimetic scaffold containing keratin, chitosan and gelatin was prepared by freeze drying method.
25491838	3	28	theme	prepared	422:429	arg1	scaffold					449:456	The prepared keratin composite scaffold	418:456	The prepared keratin composite scaffold	418:456	The prepared keratin composite scaffold had good structural integrity.
25491838	6	29	theme	compression	893:903	arg1	strength					905:912	compression strength	893:912	compression strength (8.5 kPa)	893:922	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	6	29	theme	compression	893:903	arg1	kPa					919:921	8.5 kPa	915:921	8.5 kPa	915:921	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	1	30	theme	motifs	288:293	arg1	activity					246:253	intrinsic biological activity	225:253	intrinsic biological activity	225:253	Keratin has gained much attention in the recent past as a biomaterial for wound healing owing to its biocompatibility, biodegradability, intrinsic biological activity and presence of cellular binding motifs.
25491838	1	30	theme	motifs	288:293	arg1	biodegradability					207:222	biodegradability	207:222	biodegradability	207:222	Keratin has gained much attention in the recent past as a biomaterial for wound healing owing to its biocompatibility, biodegradability, intrinsic biological activity and presence of cellular binding motifs.
25491838	1	30	theme	motifs	288:293	arg1	presence					259:266	presence	259:266	presence	259:266	Keratin has gained much attention in the recent past as a biomaterial for wound healing owing to its biocompatibility, biodegradability, intrinsic biological activity and presence of cellular binding motifs.
25491838	1	30	theme	motifs	288:293	arg1	biocompatibility					189:204	biocompatibility	189:204	biocompatibility	189:204	Keratin has gained much attention in the recent past as a biomaterial for wound healing owing to its biocompatibility, biodegradability, intrinsic biological activity and presence of cellular binding motifs.
25491838	8	31	theme	MTT	1186:1188	arg1	assay					1190:1194	MTT assay	1186:1194	MTT assay using NIH 3T3 fibroblast cells	1186:1225	MTT assay using NIH 3T3 fibroblast cells demonstrated that the cell viability of the keratin composite scaffold was good.
25491838	1	32	theme	much	107:110	arg1	attention					112:120	much attention	107:120	much attention	107:120	Keratin has gained much attention in the recent past as a biomaterial for wound healing owing to its biocompatibility, biodegradability, intrinsic biological activity and presence of cellular binding motifs.
25491838	3	33	theme	keratin	431:437	arg1	scaffold					449:456	The prepared keratin composite scaffold	418:456	The prepared keratin composite scaffold	418:456	The prepared keratin composite scaffold had good structural integrity.
25491838	0	34	theme	composite	40:48	arg1	scaffold					50:57	keratin-chitosan-gelatin composite scaffold	15:57	keratin-chitosan-gelatin composite scaffold for soft tissue engineering	15:85	Development of keratin-chitosan-gelatin composite scaffold for soft tissue engineering.
25491838	3	35	theme	structural	467:476	arg1	integrity					478:486	good structural integrity	462:486	good structural integrity	462:486	The prepared keratin composite scaffold had good structural integrity.
25491838	0	36	theme	keratin-chitosan-gelatin	15:38	arg1	scaffold					50:57	keratin-chitosan-gelatin composite scaffold	15:57	keratin-chitosan-gelatin composite scaffold for soft tissue engineering	15:85	Development of keratin-chitosan-gelatin composite scaffold for soft tissue engineering.
25491838	4	37	dep	Fourier	489:495	arg1	Transform					497:505	Transform	497:505	Transform Infrared (FTIR) spectroscopy	497:534	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	5	38	theme	Differential	699:710	arg1	DSC					734:736	DSC	734:736	DSC	734:736	Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) results revealed a high thermal denaturation temperature of the scaffold (200-250°C).
25491838	5	38	theme	Differential	699:710	arg1	Calorimetry					721:731	Differential Scanning Calorimetry	699:731	Differential Scanning Calorimetry (DSC)	699:737	Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) results revealed a high thermal denaturation temperature of the scaffold (200-250°C).
25491838	7	39	theme	Scanning	1095:1102	arg1	Microscope					1113:1122	a Scanning Electron Microscope	1093:1122	a Scanning Electron Microscope (SEM)	1093:1128	The morphology of the keratin composite scaffold observed using a Scanning Electron Microscope (SEM) exhibited good porosity and interconnectivity of pores.
25491838	7	39	theme	Scanning	1095:1102	arg1	SEM					1125:1127	SEM	1125:1127	SEM	1125:1127	The morphology of the keratin composite scaffold observed using a Scanning Electron Microscope (SEM) exhibited good porosity and interconnectivity of pores.
25491838	8	40	theme	NIH	1202:1204	arg1	cells					1221:1225	NIH 3T3 fibroblast cells	1202:1225	NIH 3T3 fibroblast cells	1202:1225	MTT assay using NIH 3T3 fibroblast cells demonstrated that the cell viability of the keratin composite scaffold was good.
25491838	5	41	theme	denaturation	771:782	arg1	temperature					784:794	a high thermal denaturation temperature	756:794	a high thermal denaturation temperature of the scaffold (200-250°C)	756:822	Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) results revealed a high thermal denaturation temperature of the scaffold (200-250°C).
25491838	4	42	theme	structure	571:579	arg1	retention					547:555	the retention	543:555	the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold	543:659	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	5	43	theme	Scanning	712:719	arg1	DSC					734:736	DSC	734:736	DSC	734:736	Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) results revealed a high thermal denaturation temperature of the scaffold (200-250°C).
25491838	5	43	theme	Scanning	712:719	arg1	Calorimetry					721:731	Differential Scanning Calorimetry	699:731	Differential Scanning Calorimetry (DSC)	699:737	Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) results revealed a high thermal denaturation temperature of the scaffold (200-250°C).
25491838	3	44	contain	had	458:460	arg2	integrity					478:486	good structural integrity	462:486	good structural integrity	462:486	The prepared keratin composite scaffold had good structural integrity.
25491838	3	44	contain	had	458:460	arg1	scaffold					449:456	The prepared keratin composite scaffold	418:456	The prepared keratin composite scaffold	418:456	The prepared keratin composite scaffold had good structural integrity.
25491838	9	45	theme	tissue	1431:1436	arg1	applications					1450:1461	tissue engineering applications	1431:1461	tissue engineering applications	1431:1461	These observations suggest that the keratin-chitosan-gelatin composite scaffold is a promising alternative biomaterial for tissue engineering applications.
25491838	3	46	theme	composite	439:447	arg1	scaffold					449:456	The prepared keratin composite scaffold	418:456	The prepared keratin composite scaffold	418:456	The prepared keratin composite scaffold had good structural integrity.
25491838	6	47	dep	exhibited	856:864	arg1	used					1012:1015	used	1012:1015	was used as control	1008:1026	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	0	48	theme	scaffold	50:57	arg1	Development					0:10	Development	0:10	Development of keratin-chitosan-gelatin composite scaffold for soft tissue engineering.	0:86	Development of keratin-chitosan-gelatin composite scaffold for soft tissue engineering.
25491838	4	49	dep	biopolymers	595:605	arg1	chitosan					617:624	chitosan	617:624	chitosan	617:624	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	4	49	dep	biopolymers	595:605	arg1	gelatin					631:637	gelatin	631:637	gelatin	631:637	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	4	49	dep	biopolymers	595:605	arg1	keratin					608:614	keratin	608:614	keratin	608:614	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	4	49	dep	biopolymers	595:605	arg1	biopolymers					595:605	individual biopolymers	584:605	individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold	584:659	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	7	50	theme	Electron	1104:1111	arg1	Microscope					1113:1122	a Scanning Electron Microscope	1093:1122	a Scanning Electron Microscope (SEM)	1093:1128	The morphology of the keratin composite scaffold observed using a Scanning Electron Microscope (SEM) exhibited good porosity and interconnectivity of pores.
25491838	7	50	theme	Electron	1104:1111	arg1	SEM					1125:1127	SEM	1125:1127	SEM	1125:1127	The morphology of the keratin composite scaffold observed using a Scanning Electron Microscope (SEM) exhibited good porosity and interconnectivity of pores.
25491838	2	51	theme	drying	403:408	arg1	method					410:415	freeze drying method	396:415	freeze drying method	396:415	In this paper, a novel biomimetic scaffold containing keratin, chitosan and gelatin was prepared by freeze drying method.
25491838	0	52	theme	tissue	68:73	arg1	engineering					75:85	soft tissue engineering	63:85	soft tissue engineering	63:85	Development of keratin-chitosan-gelatin composite scaffold for soft tissue engineering.
25491838	6	53	theme	collagen	983:990	arg1	scaffold					992:999	a collagen scaffold	981:999	a collagen scaffold	981:999	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	0	54	theme	soft	63:66	arg1	engineering					75:85	soft tissue engineering	63:85	soft tissue engineering	63:85	Development of keratin-chitosan-gelatin composite scaffold for soft tissue engineering.
25491838	6	55	theme	composite	837:845	arg1	scaffold					847:854	The keratin composite scaffold	825:854	The keratin composite scaffold	825:854	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	6	56	theme	keratin	829:835	arg1	scaffold					847:854	The keratin composite scaffold	825:854	The keratin composite scaffold	825:854	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	8	57	theme	cell	1249:1252	arg1	good					1302:1305	good	1302:1305	good	1302:1305	MTT assay using NIH 3T3 fibroblast cells demonstrated that the cell viability of the keratin composite scaffold was good.
25491838	8	57	theme	cell	1249:1252	arg1	viability					1254:1262	the cell viability	1245:1262	the cell viability of the keratin composite scaffold	1245:1296	MTT assay using NIH 3T3 fibroblast cells demonstrated that the cell viability of the keratin composite scaffold was good.
25491838	1	58	theme	intrinsic	225:233	arg1	activity					246:253	intrinsic biological activity	225:253	intrinsic biological activity	225:253	Keratin has gained much attention in the recent past as a biomaterial for wound healing owing to its biocompatibility, biodegradability, intrinsic biological activity and presence of cellular binding motifs.
25491838	4	59	dep	Infrared	507:514	arg1	FTIR					517:520	FTIR	517:520	FTIR	517:520	Fourier Transform Infrared (FTIR) spectroscopy showed the retention of the native structure of individual biopolymers (keratin, chitosan, and gelatin) used in the scaffold.
25491838	2	60	theme	biomimetic	319:328	arg1	scaffold					330:337	a novel biomimetic scaffold	311:337	a novel biomimetic scaffold containing keratin, chitosan and gelatin	311:378	In this paper, a novel biomimetic scaffold containing keratin, chitosan and gelatin was prepared by freeze drying method.
25491838	1	61	theme	biological	235:244	arg1	activity					246:253	intrinsic biological activity	225:253	intrinsic biological activity	225:253	Keratin has gained much attention in the recent past as a biomaterial for wound healing owing to its biocompatibility, biodegradability, intrinsic biological activity and presence of cellular binding motifs.
25491838	6	62	theme	tensile	866:872	arg1	kPa					887:889	96 kPa	884:889	96 kPa	884:889	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	6	62	theme	tensile	866:872	arg1	strength					874:881	tensile strength	866:881	tensile strength (96 kPa)	866:890	The keratin composite scaffold exhibited tensile strength (96 kPa), compression strength (8.5 kPa) and water uptake capacity (>1700%) comparable to that of a collagen scaffold, which was used as control.
25491838	3	63	theme	good	462:465	arg1	integrity					478:486	good structural integrity	462:486	good structural integrity	462:486	The prepared keratin composite scaffold had good structural integrity.
25491838	8	64	theme	fibroblast	1210:1219	arg1	cells					1221:1225	NIH 3T3 fibroblast cells	1202:1225	NIH 3T3 fibroblast cells	1202:1225	MTT assay using NIH 3T3 fibroblast cells demonstrated that the cell viability of the keratin composite scaffold was good.
25491838	8	65	theme	3T3	1206:1208	arg1	cells					1221:1225	NIH 3T3 fibroblast cells	1202:1225	NIH 3T3 fibroblast cells	1202:1225	MTT assay using NIH 3T3 fibroblast cells demonstrated that the cell viability of the keratin composite scaffold was good.
25491838	9	66	theme	keratin-chitosan-gelatin	1344:1367	arg1	alternative					1403:1413	a promising alternative	1391:1413	a promising alternative biomaterial for tissue engineering applications	1391:1461	These observations suggest that the keratin-chitosan-gelatin composite scaffold is a promising alternative biomaterial for tissue engineering applications.
25491838	9	66	theme	keratin-chitosan-gelatin	1344:1367	arg1	scaffold					1379:1386	the keratin-chitosan-gelatin composite scaffold	1340:1386	the keratin-chitosan-gelatin composite scaffold	1340:1386	These observations suggest that the keratin-chitosan-gelatin composite scaffold is a promising alternative biomaterial for tissue engineering applications.
25491838	7	67	theme	pores	1179:1183	arg1	interconnectivity					1158:1174	interconnectivity	1158:1174	interconnectivity	1158:1174	The morphology of the keratin composite scaffold observed using a Scanning Electron Microscope (SEM) exhibited good porosity and interconnectivity of pores.
25491838	7	67	theme	pores	1179:1183	arg1	porosity					1145:1152	good porosity	1140:1152	good porosity	1140:1152	The morphology of the keratin composite scaffold observed using a Scanning Electron Microscope (SEM) exhibited good porosity and interconnectivity of pores.
25491838	9	68	theme	composite	1369:1377	arg1	alternative					1403:1413	a promising alternative	1391:1413	a promising alternative biomaterial for tissue engineering applications	1391:1461	These observations suggest that the keratin-chitosan-gelatin composite scaffold is a promising alternative biomaterial for tissue engineering applications.
25491838	9	68	theme	composite	1369:1377	arg1	scaffold					1379:1386	the keratin-chitosan-gelatin composite scaffold	1340:1386	the keratin-chitosan-gelatin composite scaffold	1340:1386	These observations suggest that the keratin-chitosan-gelatin composite scaffold is a promising alternative biomaterial for tissue engineering applications.
25491838	7	69	theme	keratin	1051:1057	arg1	scaffold					1069:1076	the keratin composite scaffold	1047:1076	the keratin composite scaffold observed using a Scanning Electron Microscope (SEM)	1047:1128	The morphology of the keratin composite scaffold observed using a Scanning Electron Microscope (SEM) exhibited good porosity and interconnectivity of pores.
25208224	4	0	theme	single-platform	596:610	arg1	workflow					620:627	This convenient, reliable, and accurate single-platform (GC/MS) workflow	556:627	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition	556:661	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition offers significant advantages over existing methods.
25208224	4	1	theme	GC/MS	613:617	arg1	workflow					620:627	This convenient, reliable, and accurate single-platform (GC/MS) workflow	556:627	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition	556:661	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition offers significant advantages over existing methods.
25208224	1	2	theme	microbial	154:162	arg1	biomass					164:170	microbial biomass	154:170	microbial biomass	154:170	We developed a set of methods for the quantification of four major components of microbial biomass using gas chromatography/mass spectrometry (GC/MS).
25208224	1	3	theme	biomass	164:170	arg1	components					140:149	four major components	129:149	four major components of microbial biomass	129:170	We developed a set of methods for the quantification of four major components of microbial biomass using gas chromatography/mass spectrometry (GC/MS).
25208224	6	4	dep	systems	921:927	arg1	systems					921:927	systems microbiology and bioengineering	921:959	systems microbiology and bioengineering	921:959	The presented methods will have widespread applicability in systems microbiology and bioengineering.
25208224	6	4	dep	systems	921:927	arg1	bioengineering					946:959	bioengineering	946:959	bioengineering	946:959	The presented methods will have widespread applicability in systems microbiology and bioengineering.
25208224	6	4	dep	systems	921:927	arg1	microbiology					929:940	microbiology	929:940	microbiology	929:940	The presented methods will have widespread applicability in systems microbiology and bioengineering.
25208224	3	5	dep	fully	447:451	arg1	C-labeled					457:465	C-labeled	457:465	C-labeled	457:465	Quantification is performed by isotope ratio analysis with fully (13)C-labeled biomass as internal standard, which is generated by growing E. coli on [U-(13)C]glucose.
25208224	4	6	theme	reliable	573:580	arg1	workflow					620:627	This convenient, reliable, and accurate single-platform (GC/MS) workflow	556:627	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition	556:661	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition offers significant advantages over existing methods.
25208224	2	7	theme	estimated	335:343	arg1	weight					360:365	the dry weight	352:365	the dry weight of Escherichia coli	352:385	Specifically, methods are described to quantify amino acids, RNA, fatty acids, and glycogen, which comprise an estimated 88% of the dry weight of Escherichia coli.
25208224	2	7	theme	estimated	335:343	arg1	%					347:347	an estimated 88%	332:347	an estimated 88% of the dry weight of Escherichia coli	332:385	Specifically, methods are described to quantify amino acids, RNA, fatty acids, and glycogen, which comprise an estimated 88% of the dry weight of Escherichia coli.
25208224	5	8	theme	coli	847:850	arg1	strains					852:858	E. coli strains	844:858	three metabolically unique E. coli strains	817:858	We demonstrate the consistency, accuracy, precision, and utility of this procedure by applying it to three metabolically unique E. coli strains.
25208224	5	9	theme	procedure	789:797	arg1	precision					758:766	precision	758:766	precision	758:766	We demonstrate the consistency, accuracy, precision, and utility of this procedure by applying it to three metabolically unique E. coli strains.
25208224	5	9	theme	procedure	789:797	arg1	utility					773:779	utility	773:779	utility	773:779	We demonstrate the consistency, accuracy, precision, and utility of this procedure by applying it to three metabolically unique E. coli strains.
25208224	5	9	theme	procedure	789:797	arg1	accuracy					748:755	accuracy	748:755	accuracy	748:755	We demonstrate the consistency, accuracy, precision, and utility of this procedure by applying it to three metabolically unique E. coli strains.
25208224	5	9	theme	procedure	789:797	arg1	consistency					735:745	the consistency	731:745	the consistency	731:745	We demonstrate the consistency, accuracy, precision, and utility of this procedure by applying it to three metabolically unique E. coli strains.
25208224	2	10	theme	weight	360:365	arg1	weight					360:365	the dry weight	352:365	the dry weight of Escherichia coli	352:385	Specifically, methods are described to quantify amino acids, RNA, fatty acids, and glycogen, which comprise an estimated 88% of the dry weight of Escherichia coli.
25208224	2	10	theme	weight	360:365	arg1	%					347:347	an estimated 88%	332:347	an estimated 88% of the dry weight of Escherichia coli	332:385	Specifically, methods are described to quantify amino acids, RNA, fatty acids, and glycogen, which comprise an estimated 88% of the dry weight of Escherichia coli.
25208224	1	11	theme	gas	178:180	arg1	GC/MS					216:220	GC/MS	216:220	GC/MS	216:220	We developed a set of methods for the quantification of four major components of microbial biomass using gas chromatography/mass spectrometry (GC/MS).
25208224	1	11	theme	gas	178:180	arg1	spectrometry					202:213	gas chromatography/mass spectrometry	178:213	gas chromatography/mass spectrometry (GC/MS)	178:221	We developed a set of methods for the quantification of four major components of microbial biomass using gas chromatography/mass spectrometry (GC/MS).
25208224	0	12	theme	biomass	12:18	arg1	composition					20:30	biomass composition	12:30	biomass composition	12:30	Quantifying biomass composition by gas chromatography/mass spectrometry.
25208224	1	13	theme	chromatography/mass	182:200	arg1	GC/MS					216:220	GC/MS	216:220	GC/MS	216:220	We developed a set of methods for the quantification of four major components of microbial biomass using gas chromatography/mass spectrometry (GC/MS).
25208224	1	13	theme	chromatography/mass	182:200	arg1	spectrometry					202:213	gas chromatography/mass spectrometry	178:213	gas chromatography/mass spectrometry (GC/MS)	178:221	We developed a set of methods for the quantification of four major components of microbial biomass using gas chromatography/mass spectrometry (GC/MS).
25208224	6	14	theme	widespread	893:902	arg1	applicability					904:916	widespread applicability	893:916	widespread applicability	893:916	The presented methods will have widespread applicability in systems microbiology and bioengineering.
25208224	1	15	theme	methods	95:101	arg1	methods					95:101	methods	95:101	methods for the quantification of four major components of microbial biomass	95:170	We developed a set of methods for the quantification of four major components of microbial biomass using gas chromatography/mass spectrometry (GC/MS).
25208224	1	15	theme	methods	95:101	arg1	set					88:90	a set	86:90	a set of methods for the quantification of four major components of microbial biomass	86:170	We developed a set of methods for the quantification of four major components of microbial biomass using gas chromatography/mass spectrometry (GC/MS).
25208224	4	16	theme	significant	670:680	arg1	advantages					682:691	significant advantages	670:691	significant advantages	670:691	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition offers significant advantages over existing methods.
25208224	4	17	theme	biomass	643:649	arg1	composition					651:661	biomass composition	643:661	biomass composition	643:661	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition offers significant advantages over existing methods.
25208224	2	18	theme	fatty	290:294	arg1	acids					296:300	fatty acids	290:300	fatty acids	290:300	Specifically, methods are described to quantify amino acids, RNA, fatty acids, and glycogen, which comprise an estimated 88% of the dry weight of Escherichia coli.
25208224	2	19	theme	coli	382:385	arg1	weight					360:365	the dry weight	352:365	the dry weight of Escherichia coli	352:385	Specifically, methods are described to quantify amino acids, RNA, fatty acids, and glycogen, which comprise an estimated 88% of the dry weight of Escherichia coli.
25208224	0	20	theme	chromatography/mass	39:57	arg1	spectrometry					59:70	gas chromatography/mass spectrometry	35:70	gas chromatography/mass spectrometry	35:70	Quantifying biomass composition by gas chromatography/mass spectrometry.
25208224	3	21	with	analysis	433:440	arg1	biomass					467:473	fully (13)C-labeled biomass	447:473	fully (13)C-labeled biomass	447:473	Quantification is performed by isotope ratio analysis with fully (13)C-labeled biomass as internal standard, which is generated by growing E. coli on [U-(13)C]glucose.
25208224	4	22	theme	convenient	561:570	arg1	workflow					620:627	This convenient, reliable, and accurate single-platform (GC/MS) workflow	556:627	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition	556:661	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition offers significant advantages over existing methods.
25208224	3	23	theme	[U-	538:540	arg1	glucose					547:553	[U-(13)C]glucose	538:553	[U-(13)C]glucose	538:553	Quantification is performed by isotope ratio analysis with fully (13)C-labeled biomass as internal standard, which is generated by growing E. coli on [U-(13)C]glucose.
25208224	3	24	theme	isotope	419:425	arg1	analysis					433:440	isotope ratio analysis	419:440	isotope ratio analysis with fully (13)C-labeled biomass	419:473	Quantification is performed by isotope ratio analysis with fully (13)C-labeled biomass as internal standard, which is generated by growing E. coli on [U-(13)C]glucose.
25208224	0	25	theme	gas	35:37	arg1	spectrometry					59:70	gas chromatography/mass spectrometry	35:70	gas chromatography/mass spectrometry	35:70	Quantifying biomass composition by gas chromatography/mass spectrometry.
25208224	6	26	theme	presented	865:873	arg1	methods					875:881	The presented methods	861:881	The presented methods	861:881	The presented methods will have widespread applicability in systems microbiology and bioengineering.
25208224	3	27	theme	ratio	427:431	arg1	analysis					433:440	isotope ratio analysis	419:440	isotope ratio analysis with fully (13)C-labeled biomass	419:473	Quantification is performed by isotope ratio analysis with fully (13)C-labeled biomass as internal standard, which is generated by growing E. coli on [U-(13)C]glucose.
25208224	6	28	contain	have	888:891	arg2	applicability					904:916	widespread applicability	893:916	widespread applicability	893:916	The presented methods will have widespread applicability in systems microbiology and bioengineering.
25208224	6	28	contain	have	888:891	arg1	methods					875:881	The presented methods	861:881	The presented methods	861:881	The presented methods will have widespread applicability in systems microbiology and bioengineering.
25208224	5	29	theme	unique	837:842	arg1	strains					852:858	E. coli strains	844:858	three metabolically unique E. coli strains	817:858	We demonstrate the consistency, accuracy, precision, and utility of this procedure by applying it to three metabolically unique E. coli strains.
25208224	3	30	theme	C	545:545	arg1	glucose					547:553	[U-(13)C]glucose	538:553	[U-(13)C]glucose	538:553	Quantification is performed by isotope ratio analysis with fully (13)C-labeled biomass as internal standard, which is generated by growing E. coli on [U-(13)C]glucose.
25208224	2	31	theme	amino	272:276	arg1	acids					278:282	amino acids	272:282	amino acids	272:282	Specifically, methods are described to quantify amino acids, RNA, fatty acids, and glycogen, which comprise an estimated 88% of the dry weight of Escherichia coli.
25208224	3	32	theme	fully	447:451	arg1	biomass					467:473	fully (13)C-labeled biomass	447:473	fully (13)C-labeled biomass	447:473	Quantification is performed by isotope ratio analysis with fully (13)C-labeled biomass as internal standard, which is generated by growing E. coli on [U-(13)C]glucose.
25208224	1	33	theme	major	134:138	arg1	components					140:149	four major components	129:149	four major components of microbial biomass	129:170	We developed a set of methods for the quantification of four major components of microbial biomass using gas chromatography/mass spectrometry (GC/MS).
25208224	5	34	theme	E.	844:845	arg1	strains					852:858	E. coli strains	844:858	three metabolically unique E. coli strains	817:858	We demonstrate the consistency, accuracy, precision, and utility of this procedure by applying it to three metabolically unique E. coli strains.
25208224	1	35	theme	components	140:149	arg1	quantification					111:124	the quantification	107:124	the quantification of four major components of microbial biomass	107:170	We developed a set of methods for the quantification of four major components of microbial biomass using gas chromatography/mass spectrometry (GC/MS).
25208224	2	36	theme	dry	356:358	arg1	weight					360:365	the dry weight	352:365	the dry weight of Escherichia coli	352:385	Specifically, methods are described to quantify amino acids, RNA, fatty acids, and glycogen, which comprise an estimated 88% of the dry weight of Escherichia coli.
25208224	4	37	theme	accurate	587:594	arg1	workflow					620:627	This convenient, reliable, and accurate single-platform (GC/MS) workflow	556:627	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition	556:661	This convenient, reliable, and accurate single-platform (GC/MS) workflow for measuring biomass composition offers significant advantages over existing methods.
26033744	4	0	theme	reduced	800:806	arg1	energy					814:819	reduced feces energy	800:819	reduced feces energy	800:819	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	6	1	theme	additional	1012:1021	arg1	energy					1023:1028	The additional energy	1008:1028	The additional energy provided by the inulin diet	1008:1056	The additional energy provided by the inulin diet led to an increased bacterial proliferation in this group.
26033744	10	2	theme	odd-chain	1621:1629	arg1	acids					1637:1641	omega-3 and odd-chain fatty acids	1609:1641	acids	1637:1641	Concentrations of omega-3 and odd-chain fatty acids were increased in inulin-fed mice, whereas omega-6 fatty acids were reduced.
26033744	2	3	theme	C3H/HeOuJ	543:551	arg1	mice					553:556	gnotobiotic C3H/HeOuJ mice	531:556	gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota	531:601	We aimed to answer this paradox by investigating the effects of fermentable and non-fermentable fibers on obesity induced by high-fat diet in gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota.
26033744	10	4	theme	inulin-fed	1661:1670	arg1	mice					1672:1675	inulin-fed mice	1661:1675	inulin-fed mice	1661:1675	Concentrations of omega-3 and odd-chain fatty acids were increased in inulin-fed mice, whereas omega-6 fatty acids were reduced.
26033744	4	5	from	differences	861:871	arg1	intake					881:886	food intake	876:886	food intake	876:886	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	4	6	theme	increased	767:775	arg1	digestibility					782:794	an increased diet digestibility	764:794	an increased diet digestibility	764:794	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	2	7	from	effects	442:448	arg1	obesity					495:501	obesity	495:501	obesity induced by high-fat diet in gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota	495:601	We aimed to answer this paradox by investigating the effects of fermentable and non-fermentable fibers on obesity induced by high-fat diet in gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota.
26033744	3	8	theme	%	661:661	arg1	cellulose					663:671	10% cellulose	659:671	10% cellulose (non-fermentable)	659:689	Mice were fed a high-fat diet supplemented either with 10% cellulose (non-fermentable) or inulin (fermentable) for 6 weeks.
26033744	11	9	theme	obesity	1898:1904	arg1	development					1906:1916	obesity development	1898:1916	obesity development	1898:1916	Taken together, these data indicate that, during this short-term feeding, inulin has mainly positive effects on the lipid metabolism, which could cause beneficial effects during obesity development in long-term studies.
26033744	6	10	theme	increased	1068:1076	arg1	proliferation					1088:1100	an increased bacterial proliferation	1065:1100	an increased bacterial proliferation in this group	1065:1114	The additional energy provided by the inulin diet led to an increased bacterial proliferation in this group.
26033744	1	11	theme	fibers	179:184	arg1	effects					160:166	contradictory effects	146:166	contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA),	146:249	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	4	12	dep	resulted	752:759	arg1	compared					822:829	compared	822:829	compared	822:829	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	1	13	theme	products	209:216	arg1	effects					160:166	contradictory effects	146:166	contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA),	146:249	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	2	14	theme	high-fat	514:521	arg1	diet					523:526	high-fat diet	514:526	high-fat diet	514:526	We aimed to answer this paradox by investigating the effects of fermentable and non-fermentable fibers on obesity induced by high-fat diet in gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota.
26033744	8	15	theme	acid	1370:1373	arg1	elongation/desaturation					1375:1397	fatty acid elongation/desaturation	1364:1397	fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2)	1364:1445	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	8	16	dep	lipogenesis	1335:1345	arg1	Gpam					1354:1357	Gpam	1354:1357	Gpam	1354:1357	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	8	16	dep	lipogenesis	1335:1345	arg1	Fasn					1348:1351	Fasn	1348:1351	Fasn	1348:1351	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	7	17	theme	cecal	1263:1267	arg1	ratio					1288:1292	a reduced cecal acetate:propionate ratio	1253:1292	a reduced cecal acetate:propionate ratio	1253:1292	Supplementation of inulin resulted further in significantly elevated concentrations of total SCFA in cecum and portal vein plasma, with a reduced cecal acetate:propionate ratio.
26033744	5	18	from	increase	979:986	arg1	fat/weight					996:1005	body fat/weight	991:1005	body fat/weight	991:1005	However, we observed no increase in body fat/weight.
26033744	4	19	theme	increased	903:911	arg1	extraction					931:940	an increased intestinal energy extraction	900:940	an increased intestinal energy extraction from inulin	900:952	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	0	20	theme	acid	65:68	arg1	production					70:79	short-chain fatty acid production	47:79	short-chain fatty acid production	47:79	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	1	21	theme	contradictory	146:158	arg1	effects					160:166	contradictory effects	146:166	contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA),	146:249	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	2	22	theme	non-fermentable	469:483	arg1	fibers					485:490	fermentable and non-fermentable fibers	453:490	fermentable and non-fermentable fibers	453:490	We aimed to answer this paradox by investigating the effects of fermentable and non-fermentable fibers on obesity induced by high-fat diet in gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota.
26033744	0	23	from	Effects	0:6	arg1	production					70:79	short-chain fatty acid production	47:79	short-chain fatty acid production	47:79	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	0	23	from	Effects	0:6	arg1	growth					39:44	bacterial growth	29:44	bacterial growth	29:44	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	0	23	from	Effects	0:6	arg1	metabolism					99:108	hepatic lipid metabolism	85:108	hepatic lipid metabolism	85:108	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	2	24	theme	human	586:590	arg1	microbiota					592:601	a simplified human microbiota	573:601	a simplified human microbiota	573:601	We aimed to answer this paradox by investigating the effects of fermentable and non-fermentable fibers on obesity induced by high-fat diet in gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota.
26033744	2	25	theme	fermentable	453:463	arg1	fibers					485:490	fermentable and non-fermentable fibers	453:490	fermentable and non-fermentable fibers	453:490	We aimed to answer this paradox by investigating the effects of fermentable and non-fermentable fibers on obesity induced by high-fat diet in gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota.
26033744	0	26	from	growth	39:44	arg1	mice					125:128	gnotobiotic mice	113:128	gnotobiotic mice	113:128	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	0	27	theme	lipid	93:97	arg1	metabolism					99:108	hepatic lipid metabolism	85:108	hepatic lipid metabolism	85:108	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	1	28	theme	other	313:317	arg1	hand					319:322	the other hand	309:322	the other hand	309:322	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	7	29	dep	cecum	1218:1222	arg1	plasma					1240:1245	plasma	1240:1245	plasma	1240:1245	Supplementation of inulin resulted further in significantly elevated concentrations of total SCFA in cecum and portal vein plasma, with a reduced cecal acetate:propionate ratio.
26033744	7	30	theme	reduced	1255:1261	arg1	ratio					1288:1292	a reduced cecal acetate:propionate ratio	1253:1292	a reduced cecal acetate:propionate ratio	1253:1292	Supplementation of inulin resulted further in significantly elevated concentrations of total SCFA in cecum and portal vein plasma, with a reduced cecal acetate:propionate ratio.
26033744	11	31	contain	has	1801:1803	arg2	effects					1821:1827	mainly positive effects	1805:1827	mainly positive effects	1805:1827	Taken together, these data indicate that, during this short-term feeding, inulin has mainly positive effects on the lipid metabolism, which could cause beneficial effects during obesity development in long-term studies.
26033744	11	31	contain	has	1801:1803	arg1	inulin					1794:1799	inulin	1794:1799	inulin	1794:1799	Taken together, these data indicate that, during this short-term feeding, inulin has mainly positive effects on the lipid metabolism, which could cause beneficial effects during obesity development in long-term studies.
26033744	9	32	theme	different	1565:1573	arg1	groups					1583:1588	the different feeding groups	1561:1588	the different feeding groups	1561:1588	Accordingly, plasma and liver phospholipid composition were changed between the different feeding groups.
26033744	0	33	theme	dietary	11:17	arg1	inulin					19:24	dietary inulin	11:24	dietary inulin	11:24	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	8	34	dep	elongation/desaturation	1375:1397	arg1	Elovl5					1422:1427	Elovl5	1422:1427	Elovl5	1422:1427	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	8	34	dep	elongation/desaturation	1375:1397	arg1	Fads1					1430:1434	Fads1	1430:1434	Fads1	1430:1434	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	8	34	dep	elongation/desaturation	1375:1397	arg1	Elovl3					1406:1411	Elovl3	1406:1411	Elovl3	1406:1411	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	8	34	dep	elongation/desaturation	1375:1397	arg1	Elovl6					1414:1419	Elovl6	1414:1419	Elovl6	1414:1419	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	8	34	dep	elongation/desaturation	1375:1397	arg1	Fads2					1440:1444	Fads2	1440:1444	Fads2	1440:1444	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	8	34	dep	elongation/desaturation	1375:1397	arg1	Scd1					1400:1403	Scd1	1400:1403	Scd1	1400:1403	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	4	35	theme	energy	924:929	arg1	extraction					931:940	an increased intestinal energy extraction	900:940	an increased intestinal energy extraction from inulin	900:952	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	8	36	theme	genes	1317:1321	arg1	expression					1303:1312	Hepatic expression	1295:1312	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2)	1295:1445	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	11	37	theme	positive	1812:1819	arg1	effects					1821:1827	mainly positive effects	1805:1827	mainly positive effects	1805:1827	Taken together, these data indicate that, during this short-term feeding, inulin has mainly positive effects on the lipid metabolism, which could cause beneficial effects during obesity development in long-term studies.
26033744	7	38	theme	portal	1228:1233	arg1	vein					1235:1238	portal vein	1228:1238	portal vein	1228:1238	Supplementation of inulin resulted further in significantly elevated concentrations of total SCFA in cecum and portal vein plasma, with a reduced cecal acetate:propionate ratio.
26033744	0	39	theme	short-chain	47:57	arg1	production					70:79	short-chain fatty acid production	47:79	short-chain fatty acid production	47:79	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	9	40	theme	feeding	1575:1581	arg1	groups					1583:1588	the different feeding groups	1561:1588	the different feeding groups	1561:1588	Accordingly, plasma and liver phospholipid composition were changed between the different feeding groups.
26033744	6	41	theme	inulin	1046:1051	arg1	diet					1053:1056	the inulin diet	1042:1056	the inulin diet	1042:1056	The additional energy provided by the inulin diet led to an increased bacterial proliferation in this group.
26033744	8	42	theme	inulin-fed	1465:1474	arg1	animals					1476:1482	inulin-fed animals	1465:1482	inulin-fed animals	1465:1482	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	7	43	theme	SCFA	1210:1213	arg1	concentrations					1186:1199	significantly elevated concentrations	1163:1199	significantly elevated concentrations of total SCFA	1163:1213	Supplementation of inulin resulted further in significantly elevated concentrations of total SCFA in cecum and portal vein plasma, with a reduced cecal acetate:propionate ratio.
26033744	11	44	theme	short-term	1774:1783	arg1	feeding					1785:1791	this short-term feeding	1769:1791	this short-term feeding	1769:1791	Taken together, these data indicate that, during this short-term feeding, inulin has mainly positive effects on the lipid metabolism, which could cause beneficial effects during obesity development in long-term studies.
26033744	1	45	theme	fatty	231:235	arg1	SCFA					244:247	SCFA	244:247	SCFA	244:247	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	1	45	theme	fatty	231:235	arg1	acids					237:241	short-chain fatty acids	219:241	short-chain fatty acids (SCFA)	219:248	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	1	45	theme	fatty	231:235	arg1	products					209:216	their fermentation products	190:216	their fermentation products	190:216	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	3	46	theme	10	659:660	arg1	%					661:661	%	661:661	%	661:661	Mice were fed a high-fat diet supplemented either with 10% cellulose (non-fermentable) or inulin (fermentable) for 6 weeks.
26033744	10	47	theme	fatty	1631:1635	arg1	acids					1637:1641	omega-3 and odd-chain fatty acids	1609:1641	acids	1637:1641	Concentrations of omega-3 and odd-chain fatty acids were increased in inulin-fed mice, whereas omega-6 fatty acids were reduced.
26033744	7	48	theme	elevated	1177:1184	arg1	concentrations					1186:1199	significantly elevated concentrations	1163:1199	significantly elevated concentrations of total SCFA	1163:1213	Supplementation of inulin resulted further in significantly elevated concentrations of total SCFA in cecum and portal vein plasma, with a reduced cecal acetate:propionate ratio.
26033744	2	49	theme	gnotobiotic	531:541	arg1	mice					553:556	gnotobiotic C3H/HeOuJ mice	531:556	gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota	531:601	We aimed to answer this paradox by investigating the effects of fermentable and non-fermentable fibers on obesity induced by high-fat diet in gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota.
26033744	2	50	theme	simplified	575:584	arg1	microbiota					592:601	a simplified human microbiota	573:601	a simplified human microbiota	573:601	We aimed to answer this paradox by investigating the effects of fermentable and non-fermentable fibers on obesity induced by high-fat diet in gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota.
26033744	6	51	theme	bacterial	1078:1086	arg1	proliferation					1088:1100	an increased bacterial proliferation	1065:1100	an increased bacterial proliferation in this group	1065:1114	The additional energy provided by the inulin diet led to an increased bacterial proliferation in this group.
26033744	9	52	dep	plasma	1498:1503	arg1	composition					1528:1538	phospholipid composition	1515:1538	phospholipid composition	1515:1538	Accordingly, plasma and liver phospholipid composition were changed between the different feeding groups.
26033744	10	53	theme	fatty	1694:1698	arg1	acids					1700:1704	omega-6 fatty acids	1686:1704	omega-6 fatty acids	1686:1704	Concentrations of omega-3 and odd-chain fatty acids were increased in inulin-fed mice, whereas omega-6 fatty acids were reduced.
26033744	1	54	theme	dietary	171:177	arg1	fibers					179:184	dietary fibers	171:184	dietary fibers	171:184	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	4	55	theme	diet	777:780	arg1	digestibility					782:794	an increased diet digestibility	764:794	an increased diet digestibility	764:794	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	0	56	theme	gnotobiotic	113:123	arg1	mice					125:128	gnotobiotic mice	113:128	gnotobiotic mice	113:128	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	11	57	theme	long-term	1921:1929	arg1	studies					1931:1937	long-term studies	1921:1937	long-term studies	1921:1937	Taken together, these data indicate that, during this short-term feeding, inulin has mainly positive effects on the lipid metabolism, which could cause beneficial effects during obesity development in long-term studies.
26033744	4	58	theme	cellulose	838:846	arg1	diet					848:851	the cellulose diet	834:851	the cellulose diet	834:851	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	0	59	from	production	70:79	arg1	mice					125:128	gnotobiotic mice	113:128	gnotobiotic mice	113:128	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	5	60	theme	body	991:994	arg1	fat/weight					996:1005	body fat/weight	991:1005	body fat/weight	991:1005	However, we observed no increase in body fat/weight.
26033744	1	61	theme	fermentation	196:207	arg1	acids					237:241	short-chain fatty acids	219:241	short-chain fatty acids (SCFA)	219:248	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	1	61	theme	fermentation	196:207	arg1	products					209:216	their fermentation products	190:216	their fermentation products	190:216	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	2	62	theme	fibers	485:490	arg1	effects					442:448	the effects	438:448	the effects of fermentable and non-fermentable fibers on obesity induced by high-fat diet in gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota	438:601	We aimed to answer this paradox by investigating the effects of fermentable and non-fermentable fibers on obesity induced by high-fat diet in gnotobiotic C3H/HeOuJ mice colonized with a simplified human microbiota.
26033744	8	63	theme	fatty	1364:1368	arg1	acid					1370:1373	fatty acid	1364:1373	fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2)	1364:1445	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	1	64	theme	additional	338:347	arg1	energy					349:354	additional energy	338:354	additional energy	338:354	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	11	65	theme	lipid	1836:1840	arg1	metabolism					1842:1851	the lipid metabolism	1832:1851	the lipid metabolism	1832:1851	Taken together, these data indicate that, during this short-term feeding, inulin has mainly positive effects on the lipid metabolism, which could cause beneficial effects during obesity development in long-term studies.
26033744	0	66	from	metabolism	99:108	arg1	mice					125:128	gnotobiotic mice	113:128	gnotobiotic mice	113:128	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	11	67	theme	beneficial	1872:1881	arg1	effects					1883:1889	beneficial effects	1872:1889	beneficial effects	1872:1889	Taken together, these data indicate that, during this short-term feeding, inulin has mainly positive effects on the lipid metabolism, which could cause beneficial effects during obesity development in long-term studies.
26033744	7	68	theme	propionate	1277:1286	arg1	ratio					1288:1292	a reduced cecal acetate:propionate ratio	1253:1292	a reduced cecal acetate:propionate ratio	1253:1292	Supplementation of inulin resulted further in significantly elevated concentrations of total SCFA in cecum and portal vein plasma, with a reduced cecal acetate:propionate ratio.
26033744	4	69	theme	feces	808:812	arg1	energy					814:819	reduced feces energy	800:819	reduced feces energy	800:819	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	0	70	theme	fatty	59:63	arg1	production					70:79	short-chain fatty acid production	47:79	short-chain fatty acid production	47:79	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	1	71	theme	obesity	368:374	arg1	development					376:386	obesity development	368:386	obesity development	368:386	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	7	72	theme	acetate	1269:1275	arg1	ratio					1288:1292	a reduced cecal acetate:propionate ratio	1253:1292	a reduced cecal acetate:propionate ratio	1253:1292	Supplementation of inulin resulted further in significantly elevated concentrations of total SCFA in cecum and portal vein plasma, with a reduced cecal acetate:propionate ratio.
26033744	10	73	dep	increased	1648:1656	arg1	whereas					1678:1684	whereas	1678:1684	whereas	1678:1684	Concentrations of omega-3 and odd-chain fatty acids were increased in inulin-fed mice, whereas omega-6 fatty acids were reduced.
26033744	7	74	theme	inulin	1136:1141	arg1	Supplementation					1117:1131	Supplementation	1117:1131	Supplementation of inulin	1117:1141	Supplementation of inulin resulted further in significantly elevated concentrations of total SCFA in cecum and portal vein plasma, with a reduced cecal acetate:propionate ratio.
26033744	0	75	theme	hepatic	85:91	arg1	metabolism					99:108	hepatic lipid metabolism	85:108	hepatic lipid metabolism	85:108	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	6	76	from	proliferation	1088:1100	arg1	group					1110:1114	this group	1105:1114	this group	1105:1114	The additional energy provided by the inulin diet led to an increased bacterial proliferation in this group.
26033744	4	77	theme	food	876:879	arg1	intake					881:886	food intake	876:886	food intake	876:886	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	0	78	theme	inulin	19:24	arg1	Effects					0:6	Effects	0:6	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.	0:129	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	8	79	theme	Hepatic	1295:1301	arg1	expression					1303:1312	Hepatic expression	1295:1312	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2)	1295:1445	Hepatic expression of genes involved in lipogenesis (Fasn, Gpam) and fatty acid elongation/desaturation (Scd1, Elovl3, Elovl6, Elovl5, Fads1 and Fads2) were decreased in inulin-fed animals.
26033744	0	80	theme	bacterial	29:37	arg1	growth					39:44	bacterial growth	29:44	bacterial growth	29:44	Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice.
26033744	4	81	from	inulin	947:952	arg1	extraction					931:940	an increased intestinal energy extraction	900:940	an increased intestinal energy extraction from inulin	900:952	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	4	82	theme	intestinal	913:922	arg1	extraction					931:940	an increased intestinal energy extraction	900:940	an increased intestinal energy extraction from inulin	900:952	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	4	83	theme	inulin	740:745	arg1	diet					747:750	the inulin diet	736:750	the inulin diet	736:750	Feeding the inulin diet resulted in an increased diet digestibility and reduced feces energy, compared to the cellulose diet with no differences in food intake, suggesting an increased intestinal energy extraction from inulin.
26033744	3	84	dep	cellulose	663:671	arg1	either					647:652	either	647:652	either	647:652	Mice were fed a high-fat diet supplemented either with 10% cellulose (non-fermentable) or inulin (fermentable) for 6 weeks.
26033744	3	84	dep	cellulose	663:671	arg1	non-fermentable					674:688	non-fermentable	674:688	non-fermentable	674:688	Mice were fed a high-fat diet supplemented either with 10% cellulose (non-fermentable) or inulin (fermentable) for 6 weeks.
26033744	3	85	theme	high-fat	620:627	arg1	diet					629:632	a high-fat diet	618:632	a high-fat diet supplemented either with 10% cellulose (non-fermentable) or inulin (fermentable)	618:713	Mice were fed a high-fat diet supplemented either with 10% cellulose (non-fermentable) or inulin (fermentable) for 6 weeks.
26033744	10	86	theme	omega-3	1609:1615	arg1	acids					1637:1641	omega-3 and odd-chain fatty acids	1609:1641	acids	1637:1641	Concentrations of omega-3 and odd-chain fatty acids were increased in inulin-fed mice, whereas omega-6 fatty acids were reduced.
26033744	7	87	theme	total	1204:1208	arg1	SCFA					1210:1213	total SCFA	1204:1213	total SCFA	1204:1213	Supplementation of inulin resulted further in significantly elevated concentrations of total SCFA in cecum and portal vein plasma, with a reduced cecal acetate:propionate ratio.
26033744	1	88	theme	short-chain	219:229	arg1	SCFA					244:247	SCFA	244:247	SCFA	244:247	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	1	88	theme	short-chain	219:229	arg1	acids					237:241	short-chain fatty acids	219:241	short-chain fatty acids (SCFA)	219:248	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	1	88	theme	short-chain	219:229	arg1	products					209:216	their fermentation products	190:216	their fermentation products	190:216	In literature, contradictory effects of dietary fibers and their fermentation products, short-chain fatty acids (SCFA), are described: On one hand, they increase satiety, but on the other hand, they provide additional energy and promote obesity development.
26033744	9	89	theme	phospholipid	1515:1526	arg1	composition					1528:1538	phospholipid composition	1515:1538	phospholipid composition	1515:1538	Accordingly, plasma and liver phospholipid composition were changed between the different feeding groups.
26033744	10	90	theme	acids	1637:1641	arg1	Concentrations					1591:1604	Concentrations	1591:1604	Concentrations of omega-3 and odd-chain fatty acids	1591:1641	Concentrations of omega-3 and odd-chain fatty acids were increased in inulin-fed mice, whereas omega-6 fatty acids were reduced.
29117109	2	0	theme	overlapped	504:513	arg1	structure					523:531	a randomly oriented and overlapped network structure	480:531	a randomly oriented and overlapped network structure	480:531	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
29117109	2	1	from	layer	447:451	arg1	structure					523:531	a randomly oriented and overlapped network structure	480:531	a randomly oriented and overlapped network structure	480:531	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
29117109	7	2	theme	retardants	1498:1507	arg1	development					1419:1429	the development	1415:1429	the development of efficient, cost-effective and environmentally sustainable flame retardants	1415:1507	These results provide great opportunities for the development of efficient, cost-effective and environmentally sustainable flame retardants.
29117109	1	3	theme	thin	174:177	arg1	films					179:183	thin films	174:183	thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin	174:295	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	3	4	from	%	707:707	arg1	loss					739:742	weight loss	732:742	weight loss	732:742	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	3	4	from	%	707:707	arg1	area					756:759	burning area	748:759	burning area	748:759	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	2	5	theme	flexible	359:366	arg1	papers					368:373	Lightweight and highly flexible papers	336:373	Lightweight and highly flexible papers with increased gas impermeability	336:407	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
29117109	1	6	theme	pre-adsorbed	265:276	arg1	nanoplatelets					205:217	graphene nanoplatelets	196:217	graphene nanoplatelets (GnPs)	196:224	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	1	6	theme	pre-adsorbed	265:276	arg1	GnPs					220:223	GnPs	220:223	GnPs	220:223	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	4	7	theme	retardants	936:945	arg1	presence					918:925	the presence	914:925	the presence of flame retardants	914:945	The maximum burning temperature as measured by infrared pyrometry also decreased from 834 °C to 705 °C with the presence of flame retardants.
29117109	3	8	theme	87.7	713:716	arg1	%					717:717	%	717:717	%	717:717	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	3	9	theme	flammability	564:575	arg1	properties					577:586	the thermal and flammability properties	548:586	the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials	548:645	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	3	10	dep	83.5	703:706	arg1	to					700:701	to	700:701	to	700:701	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	4	11	theme	flame	930:934	arg1	retardants					936:945	flame retardants	930:945	flame retardants	930:945	The maximum burning temperature as measured by infrared pyrometry also decreased from 834 °C to 705 °C with the presence of flame retardants.
29117109	6	12	theme	barrier	1286:1292	arg1	characteristics					1294:1308	physical barrier characteristics	1277:1308	physical barrier characteristics	1277:1308	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	3	13	theme	properties	577:586	arg1	Assessment					534:543	Assessment	534:543	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials	534:645	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	1	14	theme	alkali	283:288	arg1	lignin					290:295	alkali lignin	283:295	alkali lignin	283:295	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	6	15	theme	physical	1277:1284	arg1	characteristics					1294:1308	physical barrier characteristics	1277:1308	physical barrier characteristics	1277:1308	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	4	16	dep	temperature	826:836	arg1	measured					841:848	measured	841:848	measured by infrared pyrometry	841:870	The maximum burning temperature as measured by infrared pyrometry also decreased from 834 °C to 705 °C with the presence of flame retardants.
29117109	3	17	theme	thermal	552:558	arg1	properties					577:586	the thermal and flammability properties	548:586	the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials	548:645	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	4	18	theme	maximum	810:816	arg1	temperature					826:836	The maximum burning temperature	806:836	The maximum burning temperature as measured by infrared pyrometry	806:870	The maximum burning temperature as measured by infrared pyrometry also decreased from 834 °C to 705 °C with the presence of flame retardants.
29117109	3	19	from	reduction	719:727	arg1	loss					739:742	weight loss	732:742	weight loss	732:742	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	3	19	from	reduction	719:727	arg1	area					756:759	burning area	748:759	burning area	748:759	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	7	20	theme	flame	1492:1496	arg1	retardants					1498:1507	efficient, cost-effective and environmentally sustainable flame retardants	1434:1507	efficient, cost-effective and environmentally sustainable flame retardants	1434:1507	These results provide great opportunities for the development of efficient, cost-effective and environmentally sustainable flame retardants.
29117109	0	21	theme	Lignin-Modified	0:14	arg1	Coating					48:54	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating	0:54	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating as Efficient Flame Retardant	0:83	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating as Efficient Flame Retardant.
29117109	1	22	theme	graphene	196:203	arg1	nanoplatelets					205:217	graphene nanoplatelets	196:217	graphene nanoplatelets (GnPs)	196:224	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	1	22	theme	graphene	196:203	arg1	GnPs					220:223	GnPs	220:223	GnPs	220:223	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	0	23	theme	Carbon	16:21	arg1	Coating					48:54	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating	0:54	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating as Efficient Flame Retardant	0:83	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating as Efficient Flame Retardant.
29117109	3	24	theme	weight	732:737	arg1	loss					739:742	weight loss	732:742	weight loss	732:742	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	1	25	theme	fire	96:99	arg1	hazards					101:107	fire hazards	96:107	fire hazards	96:107	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	2	26	theme	carbon	456:461	arg1	nanomaterials					463:475	carbon nanomaterials	456:475	carbon nanomaterials	456:475	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
29117109	6	27	theme	synergistic	1216:1226	arg1	effects					1228:1234	the synergistic effects	1212:1234	the synergistic effects between GnPs, CNTs and lignin	1212:1264	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	4	28	theme	infrared	853:860	arg1	pyrometry					862:870	infrared pyrometry	853:870	infrared pyrometry	853:870	The maximum burning temperature as measured by infrared pyrometry also decreased from 834 °C to 705 °C with the presence of flame retardants.
29117109	6	29	theme	flame-retardant	1164:1178	arg1	properties					1180:1189	These outstanding flame-retardant properties	1146:1189	These outstanding flame-retardant properties	1146:1189	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	2	30	theme	gas	390:392	arg1	impermeability					394:407	increased gas impermeability	380:407	increased gas impermeability	380:407	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
29117109	0	31	theme	Hybrid	41:46	arg1	Coating					48:54	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating	0:54	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating as Efficient Flame Retardant	0:83	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating as Efficient Flame Retardant.
29117109	5	32	theme	GnPs	994:997	arg1	GnPs					994:997	GnPs	994:997	GnPs	994:997	Furthermore, papers coated with composites of GnPs and CNTs pre-adsorbed with lignin showed enhanced thermal stability and superior fire resistance than samples treated with either component alone.
29117109	5	32	theme	GnPs	994:997	arg1	composites					980:989	composites	980:989	composites of GnPs and CNTs pre-adsorbed with lignin	980:1031	Furthermore, papers coated with composites of GnPs and CNTs pre-adsorbed with lignin showed enhanced thermal stability and superior fire resistance than samples treated with either component alone.
29117109	5	32	theme	GnPs	994:997	arg1	CNTs					1003:1006	CNTs	1003:1006	CNTs	1003:1006	Furthermore, papers coated with composites of GnPs and CNTs pre-adsorbed with lignin showed enhanced thermal stability and superior fire resistance than samples treated with either component alone.
29117109	6	33	theme	material	1359:1366	arg1	characteristics					1294:1308	physical barrier characteristics	1277:1308	physical barrier characteristics	1277:1308	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	6	33	theme	material	1359:1366	arg1	management					1341:1350	thermal management	1333:1350	thermal management of the material	1333:1366	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	6	33	theme	material	1359:1366	arg1	formation					1311:1319	formation	1311:1319	formation of char	1311:1327	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	6	34	theme	outstanding	1152:1162	arg1	properties					1180:1189	These outstanding flame-retardant properties	1146:1189	These outstanding flame-retardant properties	1146:1189	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	5	35	theme	CNTs	1003:1006	arg1	GnPs					994:997	GnPs	994:997	GnPs	994:997	Furthermore, papers coated with composites of GnPs and CNTs pre-adsorbed with lignin showed enhanced thermal stability and superior fire resistance than samples treated with either component alone.
29117109	5	35	theme	CNTs	1003:1006	arg1	composites					980:989	composites	980:989	composites of GnPs and CNTs pre-adsorbed with lignin	980:1031	Furthermore, papers coated with composites of GnPs and CNTs pre-adsorbed with lignin showed enhanced thermal stability and superior fire resistance than samples treated with either component alone.
29117109	5	35	theme	CNTs	1003:1006	arg1	CNTs					1003:1006	CNTs	1003:1006	CNTs	1003:1006	Furthermore, papers coated with composites of GnPs and CNTs pre-adsorbed with lignin showed enhanced thermal stability and superior fire resistance than samples treated with either component alone.
29117109	2	36	theme	protective	436:445	arg1	layer					447:451	a protective layer	434:451	a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure	434:531	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
29117109	0	37	theme	Nanotube/Graphene	23:39	arg1	Coating					48:54	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating	0:54	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating as Efficient Flame Retardant	0:83	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating as Efficient Flame Retardant.
29117109	3	38	theme	burning	748:754	arg1	area					756:759	burning area	748:759	burning area	748:759	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	7	39	theme	cost-effective	1445:1458	arg1	retardants					1498:1507	efficient, cost-effective and environmentally sustainable flame retardants	1434:1507	efficient, cost-effective and environmentally sustainable flame retardants	1434:1507	These results provide great opportunities for the development of efficient, cost-effective and environmentally sustainable flame retardants.
29117109	3	40	theme	papers	591:596	arg1	properties					577:586	the thermal and flammability properties	548:586	the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials	548:645	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	7	41	theme	efficient	1434:1442	arg1	retardants					1498:1507	efficient, cost-effective and environmentally sustainable flame retardants	1434:1507	efficient, cost-effective and environmentally sustainable flame retardants	1434:1507	These results provide great opportunities for the development of efficient, cost-effective and environmentally sustainable flame retardants.
29117109	2	42	theme	increased	380:388	arg1	impermeability					394:407	increased gas impermeability	380:407	increased gas impermeability	380:407	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
29117109	2	43	theme	oriented	491:498	arg1	structure					523:531	a randomly oriented and overlapped network structure	480:531	a randomly oriented and overlapped network structure	480:531	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
29117109	3	44	contain	containing	598:607	arg1	papers					591:596	papers	591:596	papers containing as low as 4 wt % carbon nanomaterials	591:645	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	3	44	contain	containing	598:607	arg2	nanomaterials					633:645	as low as 4 wt % carbon nanomaterials	609:645	as low as 4 wt % carbon nanomaterials	609:645	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	5	45	theme	superior	1071:1078	arg1	resistance					1085:1094	superior fire resistance	1071:1094	superior fire resistance	1071:1094	Furthermore, papers coated with composites of GnPs and CNTs pre-adsorbed with lignin showed enhanced thermal stability and superior fire resistance than samples treated with either component alone.
29117109	4	46	theme	burning	818:824	arg1	temperature					826:836	The maximum burning temperature	806:836	The maximum burning temperature as measured by infrared pyrometry	806:870	The maximum burning temperature as measured by infrared pyrometry also decreased from 834 °C to 705 °C with the presence of flame retardants.
29117109	7	47	theme	sustainable	1480:1490	arg1	retardants					1498:1507	efficient, cost-effective and environmentally sustainable flame retardants	1434:1507	efficient, cost-effective and environmentally sustainable flame retardants	1434:1507	These results provide great opportunities for the development of efficient, cost-effective and environmentally sustainable flame retardants.
29117109	3	48	theme	self-extinguishing	657:674	arg1	behavior					676:683	self-extinguishing behavior	657:683	self-extinguishing behavior	657:683	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	3	49	theme	wt	621:622	arg1	nanomaterials					633:645	as low as 4 wt % carbon nanomaterials	609:645	as low as 4 wt % carbon nanomaterials	609:645	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	1	50	theme	multi-wall	230:239	arg1	CNTs					259:262	CNTs	259:262	CNTs	259:262	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	1	50	theme	multi-wall	230:239	arg1	nanotubes					248:256	multi-wall carbon nanotubes	230:256	multi-wall carbon nanotubes (CNTs)	230:263	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	0	51	theme	Flame	69:73	arg1	Retardant					75:83	Efficient Flame Retardant	59:83	Efficient Flame Retardant	59:83	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating as Efficient Flame Retardant.
29117109	1	52	theme	Meyer	317:321	arg1	process					327:333	a Meyer rod process	315:333	a Meyer rod process	315:333	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	6	53	theme	thermal	1333:1339	arg1	management					1341:1350	thermal management	1333:1350	thermal management of the material	1333:1366	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	7	54	theme	great	1391:1395	arg1	opportunities					1397:1409	great opportunities	1391:1409	great opportunities for the development of efficient, cost-effective and environmentally sustainable flame retardants	1391:1507	These results provide great opportunities for the development of efficient, cost-effective and environmentally sustainable flame retardants.
29117109	1	55	theme	high-value	120:129	arg1	applications					131:142	high-value applications	120:142	high-value applications of lignocellulosic materials	120:171	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	0	56	theme	Efficient	59:67	arg1	Retardant					75:83	Efficient Flame Retardant	59:83	Efficient Flame Retardant	59:83	Lignin-Modified Carbon Nanotube/Graphene Hybrid Coating as Efficient Flame Retardant.
29117109	1	57	theme	rod	323:325	arg1	process					327:333	a Meyer rod process	315:333	a Meyer rod process	315:333	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	4	58	theme	°C	896:897	arg1	°C					906:907	834 °C to 705 °C	892:907	834 °C to 705 °C	892:907	The maximum burning temperature as measured by infrared pyrometry also decreased from 834 °C to 705 °C with the presence of flame retardants.
29117109	2	59	with	papers	368:373	arg1	impermeability					394:407	increased gas impermeability	380:407	increased gas impermeability	380:407	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
29117109	5	60	theme	fire	1080:1083	arg1	resistance					1085:1094	superior fire resistance	1071:1094	superior fire resistance	1071:1094	Furthermore, papers coated with composites of GnPs and CNTs pre-adsorbed with lignin showed enhanced thermal stability and superior fire resistance than samples treated with either component alone.
29117109	6	61	theme	char	1324:1327	arg1	characteristics					1294:1308	physical barrier characteristics	1277:1308	physical barrier characteristics	1277:1308	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	6	61	theme	char	1324:1327	arg1	management					1341:1350	thermal management	1333:1350	thermal management of the material	1333:1366	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	6	61	theme	char	1324:1327	arg1	formation					1311:1319	formation	1311:1319	formation of char	1311:1327	These outstanding flame-retardant properties can be attributed to the synergistic effects between GnPs, CNTs and lignin, enhancing physical barrier characteristics, formation of char and thermal management of the material.
29117109	5	62	theme	enhanced	1040:1047	arg1	stability					1057:1065	enhanced thermal stability	1040:1065	enhanced thermal stability	1040:1065	Furthermore, papers coated with composites of GnPs and CNTs pre-adsorbed with lignin showed enhanced thermal stability and superior fire resistance than samples treated with either component alone.
29117109	1	63	with	pre-adsorbed	265:276	arg1	lignin					290:295	alkali lignin	283:295	alkali lignin	283:295	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	2	64	theme	Lightweight	336:346	arg1	papers					368:373	Lightweight and highly flexible papers	336:373	Lightweight and highly flexible papers with increased gas impermeability	336:407	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
29117109	1	65	theme	carbon	241:246	arg1	CNTs					259:262	CNTs	259:262	CNTs	259:262	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	1	65	theme	carbon	241:246	arg1	nanotubes					248:256	multi-wall carbon nanotubes	230:256	multi-wall carbon nanotubes (CNTs)	230:263	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	5	66	theme	thermal	1049:1055	arg1	stability					1057:1065	enhanced thermal stability	1040:1065	enhanced thermal stability	1040:1065	Furthermore, papers coated with composites of GnPs and CNTs pre-adsorbed with lignin showed enhanced thermal stability and superior fire resistance than samples treated with either component alone.
29117109	1	67	theme	lignocellulosic	147:161	arg1	materials					163:171	lignocellulosic materials	147:171	lignocellulosic materials	147:171	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	4	68	dep	°C	906:907	arg1	to					899:900	to	899:900	to	899:900	The maximum burning temperature as measured by infrared pyrometry also decreased from 834 °C to 705 °C with the presence of flame retardants.
29117109	3	69	theme	%	624:624	arg1	nanomaterials					633:645	as low as 4 wt % carbon nanomaterials	609:645	as low as 4 wt % carbon nanomaterials	609:645	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	1	70	theme	materials	163:171	arg1	applications					131:142	high-value applications	120:142	high-value applications of lignocellulosic materials	120:171	To reduce fire hazards and expand high-value applications of lignocellulosic materials, thin films comprising graphene nanoplatelets (GnPs) and multi-wall carbon nanotubes (CNTs) pre-adsorbed with alkali lignin were deposited by a Meyer rod process.
29117109	3	71	theme	blank	792:796	arg1	papers					798:803	the blank papers	788:803	the blank papers	788:803	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	2	72	theme	network	515:521	arg1	structure					523:531	a randomly oriented and overlapped network structure	480:531	a randomly oriented and overlapped network structure	480:531	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
29117109	3	73	theme	%	717:717	arg1	reduction					719:727	87.7% reduction	713:727	87.7% reduction in weight loss and burning area	713:759	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	3	74	theme	carbon	626:631	arg1	nanomaterials					633:645	as low as 4 wt % carbon nanomaterials	609:645	as low as 4 wt % carbon nanomaterials	609:645	Assessment of the thermal and flammability properties of papers containing as low as 4 wt % carbon nanomaterials exhibited self-extinguishing behavior and yielded up to 83.5% and 87.7% reduction in weight loss and burning area, respectively, compared to the blank papers.
29117109	2	75	theme	nanomaterials	463:475	arg1	layer					447:451	a protective layer	434:451	a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure	434:531	Lightweight and highly flexible papers with increased gas impermeability were obtained by coating a protective layer of carbon nanomaterials in a randomly oriented and overlapped network structure.
24679409	0	0	theme	stationary	80:89	arg1	phase					91:95	a cellulose-based chiral stationary phase	55:95	a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode	55:149	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	3	1	theme	enantioselective	320:335	arg1	method					342:347	The enantioselective HPLC method	316:347	The enantioselective HPLC method reported in the current Pharmacopoeias	316:386	The enantioselective HPLC method reported in the current Pharmacopoeias shows clear disadvantages with regard to the low resolution and long elution times.
24679409	4	2	from	applicability	509:521	arg1	enantioseparation					628:644	the enantioseparation	624:644	the enantioseparation of oxaliplatin	624:659	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	6	3	theme	100mm×4.6mm	903:913	arg1	column					925:930	a 100mm×4.6mm I.D. IC-3 column	901:930	a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture	901:1027	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	5	4	theme	interaction	770:780	arg1	chromatography					789:802	hydrophilic interaction liquid chromatography	758:802	enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies	741:827	Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies.
24679409	5	4	theme	interaction	770:780	arg1	HILIC					805:809	HILIC	805:809	HILIC	805:809	Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies.
24679409	0	5	theme	chiral	73:78	arg1	phase					91:95	a cellulose-based chiral stationary phase	55:95	a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode	55:149	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	0	6	from	separation	7:16	arg1	phase					91:95	a cellulose-based chiral stationary phase	55:95	a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode	55:149	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	6	7	with	separation	841:850	arg1	factor					868:873	resolution factor	857:873	resolution factor of 5.8	857:880	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	8	8	with	sensitive	1139:1147	arg1	LOD					1154:1156	LOD	1154:1156	LOD	1154:1156	The optimized method was proven to be sensitive with LOD and LOQ of the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively.
24679409	8	8	with	sensitive	1139:1147	arg1	LOQ					1162:1164	LOQ	1162:1164	LOQ	1162:1164	The optimized method was proven to be sensitive with LOD and LOQ of the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively.
24679409	3	9	theme	current	365:371	arg1	Pharmacopoeias					373:386	the current Pharmacopoeias	361:386	the current Pharmacopoeias	361:386	The enantioselective HPLC method reported in the current Pharmacopoeias shows clear disadvantages with regard to the low resolution and long elution times.
24679409	4	10	theme	oxaliplatin	649:659	arg1	enantioseparation					628:644	the enantioseparation	624:644	the enantioseparation of oxaliplatin	624:659	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	6	11	theme	100:5	1015:1019	arg1	mixture					1021:1027	acetonitrile-water 100:5 mixture	996:1027	acetonitrile-water 100:5 mixture	996:1027	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	5	12	theme	enantioselective	741:756	arg1	strategies					818:827	enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies	741:827	enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies	741:827	Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies.
24679409	5	13	theme	Experimental	662:673	arg1	results					675:681	Experimental results	662:681	Experimental results	662:681	Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies.
24679409	6	14	theme	baseline	832:839	arg1	separation					841:850	A baseline separation	830:850	A baseline separation with resolution factor of 5.8	830:880	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	6	15	theme	acetonitrile-water	996:1013	arg1	mixture					1021:1027	acetonitrile-water 100:5 mixture	996:1027	acetonitrile-water 100:5 mixture	996:1027	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	0	16	theme	interaction	112:122	arg1	chromatography					131:144	hydrophilic interaction liquid chromatography	100:144	hydrophilic interaction liquid chromatography mode	100:149	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	6	17	theme	IC-3	920:923	arg1	column					925:930	a 100mm×4.6mm I.D. IC-3 column	901:930	a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture	901:1027	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	8	18	theme	enantiomeric	1173:1184	arg1	impurity					1186:1193	the enantiomeric impurity	1169:1193	the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively	1169:1232	The optimized method was proven to be sensitive with LOD and LOQ of the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively.
24679409	3	19	theme	long	452:455	arg1	times					465:469	long elution times	452:469	long elution times	452:469	The enantioselective HPLC method reported in the current Pharmacopoeias shows clear disadvantages with regard to the low resolution and long elution times.
24679409	0	20	theme	hydrophilic	100:110	arg1	chromatography					131:144	hydrophilic interaction liquid chromatography	100:144	hydrophilic interaction liquid chromatography mode	100:149	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	6	21	theme	I.D.	915:918	arg1	column					925:930	a 100mm×4.6mm I.D. IC-3 column	901:930	a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture	901:1027	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	3	22	theme	elution	457:463	arg1	times					465:469	long elution times	452:469	long elution times	452:469	The enantioselective HPLC method reported in the current Pharmacopoeias shows clear disadvantages with regard to the low resolution and long elution times.
24679409	3	23	theme	HPLC	337:340	arg1	method					342:347	The enantioselective HPLC method	316:347	The enantioselective HPLC method reported in the current Pharmacopoeias	316:386	The enantioselective HPLC method reported in the current Pharmacopoeias shows clear disadvantages with regard to the low resolution and long elution times.
24679409	5	24	theme	liquid	782:787	arg1	chromatography					789:802	hydrophilic interaction liquid chromatography	758:802	enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies	741:827	Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies.
24679409	5	24	theme	liquid	782:787	arg1	HILIC					805:809	HILIC	805:809	HILIC	805:809	Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies.
24679409	6	25	theme	phase	976:980	arg1	temperature					943:953	the temperature	939:953	the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture	939:1027	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	0	26	theme	Direct	0:5	arg1	separation					7:16	Direct separation	0:16	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.	0:150	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	4	27	theme	chiral	565:570	arg1	CSP					590:592	CSP	590:592	CSP	590:592	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	4	27	theme	chiral	565:570	arg1	phase					583:587	polysaccharide-based chiral stationary phase	544:587	polysaccharide-based chiral stationary phase (CSP)	544:593	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	6	28	theme	resolution	857:866	arg1	factor					868:873	resolution factor	857:873	resolution factor of 5.8	857:880	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	6	29	theme	5.8	878:880	arg1	factor					868:873	resolution factor	857:873	resolution factor of 5.8	857:880	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	8	30	theme	impurity	1186:1193	arg1	LOD					1154:1156	LOD	1154:1156	LOD	1154:1156	The optimized method was proven to be sensitive with LOD and LOQ of the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively.
24679409	8	30	theme	impurity	1186:1193	arg1	LOQ					1162:1164	LOQ	1162:1164	LOQ	1162:1164	The optimized method was proven to be sensitive with LOD and LOQ of the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively.
24679409	4	31	theme	polysaccharide-based	544:563	arg1	CSP					590:592	CSP	590:592	CSP	590:592	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	4	31	theme	polysaccharide-based	544:563	arg1	phase					583:587	polysaccharide-based chiral stationary phase	544:587	polysaccharide-based chiral stationary phase (CSP)	544:593	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	0	32	theme	chromatography	131:144	arg1	mode					146:149	hydrophilic interaction liquid chromatography mode	100:149	hydrophilic interaction liquid chromatography mode	100:149	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	2	33	theme	composition	303:313	arg1	analysis					274:281	the analysis	270:281	the analysis of its enantiomeric composition	270:313	Little attention has been devoted to the analysis of its enantiomeric composition.
24679409	1	34	theme	anticancer	176:185	arg1	ingredient					221:230	an anticancer enantiopure active pharmaceutical ingredient	173:230	an anticancer enantiopure active pharmaceutical ingredient	173:230	(R,R)-oxaliplatin is an anticancer enantiopure active pharmaceutical ingredient.
24679409	1	34	theme	anticancer	176:185	arg1	-oxaliplatin					157:168	(R,R)-oxaliplatin	152:168	(R,R)-oxaliplatin	152:168	(R,R)-oxaliplatin is an anticancer enantiopure active pharmaceutical ingredient.
24679409	3	35	theme	clear	394:398	arg1	disadvantages					400:412	clear disadvantages	394:412	clear disadvantages	394:412	The enantioselective HPLC method reported in the current Pharmacopoeias shows clear disadvantages with regard to the low resolution and long elution times.
24679409	6	36	theme	40°C	958:961	arg1	temperature					943:953	the temperature	939:953	the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture	939:1027	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	0	37	theme	liquid	124:129	arg1	chromatography					131:144	hydrophilic interaction liquid chromatography	100:144	hydrophilic interaction liquid chromatography mode	100:149	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	2	38	theme	enantiomeric	290:301	arg1	composition					303:313	its enantiomeric composition	286:313	its enantiomeric composition	286:313	Little attention has been devoted to the analysis of its enantiomeric composition.
24679409	1	39	theme	enantiopure	187:197	arg1	ingredient					221:230	an anticancer enantiopure active pharmaceutical ingredient	173:230	an anticancer enantiopure active pharmaceutical ingredient	173:230	(R,R)-oxaliplatin is an anticancer enantiopure active pharmaceutical ingredient.
24679409	1	39	theme	enantiopure	187:197	arg1	-oxaliplatin					157:168	(R,R)-oxaliplatin	152:168	(R,R)-oxaliplatin	152:168	(R,R)-oxaliplatin is an anticancer enantiopure active pharmaceutical ingredient.
24679409	8	40	theme	0.07	1198:1201	arg1	impurity					1186:1193	the enantiomeric impurity	1169:1193	the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively	1169:1232	The optimized method was proven to be sensitive with LOD and LOQ of the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively.
24679409	4	41	theme	stationary	572:581	arg1	CSP					590:592	CSP	590:592	CSP	590:592	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	4	41	theme	stationary	572:581	arg1	phase					583:587	polysaccharide-based chiral stationary phase	544:587	polysaccharide-based chiral stationary phase (CSP)	544:593	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	6	42	theme	mobile	969:974	arg1	phase					976:980	a mobile phase	967:980	a mobile phase consisting of acetonitrile-water 100:5 mixture	967:1027	A baseline separation with resolution factor of 5.8 was achieved using a 100mm×4.6mm I.D. IC-3 column set at the temperature of 40°C and a mobile phase consisting of acetonitrile-water 100:5 mixture.
24679409	1	43	theme	active	199:204	arg1	ingredient					221:230	an anticancer enantiopure active pharmaceutical ingredient	173:230	an anticancer enantiopure active pharmaceutical ingredient	173:230	(R,R)-oxaliplatin is an anticancer enantiopure active pharmaceutical ingredient.
24679409	1	43	theme	active	199:204	arg1	-oxaliplatin					157:168	(R,R)-oxaliplatin	152:168	(R,R)-oxaliplatin	152:168	(R,R)-oxaliplatin is an anticancer enantiopure active pharmaceutical ingredient.
24679409	0	44	theme	enantiomers	25:35	arg1	separation					7:16	Direct separation	0:16	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.	0:150	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	3	45	theme	low	433:435	arg1	resolution					437:446	the low resolution	429:446	the low resolution	429:446	The enantioselective HPLC method reported in the current Pharmacopoeias shows clear disadvantages with regard to the low resolution and long elution times.
24679409	2	46	theme	Little	233:238	arg1	attention					240:248	Little attention	233:248	Little attention	233:248	Little attention has been devoted to the analysis of its enantiomeric composition.
24679409	7	47	theme	flow	1035:1038	arg1	rate					1040:1043	a flow rate	1033:1043	a flow rate of 1mLmin(-1)	1033:1057	At a flow rate of 1mLmin(-1) the separation was completed within 8min.
24679409	1	48	theme	pharmaceutical	206:219	arg1	ingredient					221:230	an anticancer enantiopure active pharmaceutical ingredient	173:230	an anticancer enantiopure active pharmaceutical ingredient	173:230	(R,R)-oxaliplatin is an anticancer enantiopure active pharmaceutical ingredient.
24679409	1	48	theme	pharmaceutical	206:219	arg1	-oxaliplatin					157:168	(R,R)-oxaliplatin	152:168	(R,R)-oxaliplatin	152:168	(R,R)-oxaliplatin is an anticancer enantiopure active pharmaceutical ingredient.
24679409	5	49	theme	strategies	818:827	arg1	development					726:736	the development	722:736	the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies	722:827	Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies.
24679409	8	50	theme	optimized	1105:1113	arg1	sensitive					1139:1147	sensitive	1139:1147	sensitive	1139:1147	The optimized method was proven to be sensitive with LOD and LOQ of the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively.
24679409	8	50	theme	optimized	1105:1113	arg1	method					1115:1120	The optimized method	1101:1120	The optimized method	1101:1120	The optimized method was proven to be sensitive with LOD and LOQ of the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively.
24679409	0	51	theme	oxaliplatin	40:50	arg1	enantiomers					25:35	the enantiomers	21:35	the enantiomers of oxaliplatin	21:50	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	4	52	theme	generation	533:542	arg1	applicability					509:521	the applicability	505:521	the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin	505:659	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	4	53	dep	IC-3	615:618	arg1	i.e.					596:599	i.e.	596:599	i.e.	596:599	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	1	54	dep	-oxaliplatin	157:168	arg1	R					153:153	R	153:153	R	153:153	(R,R)-oxaliplatin is an anticancer enantiopure active pharmaceutical ingredient.
24679409	1	54	dep	-oxaliplatin	157:168	arg1	R					155:155	R	155:155	R	155:155	(R,R)-oxaliplatin is an anticancer enantiopure active pharmaceutical ingredient.
24679409	4	55	theme	last	528:531	arg1	generation					533:542	a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3,	526:619	generation	533:542	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	5	56	theme	hydrophilic	758:768	arg1	chromatography					789:802	hydrophilic interaction liquid chromatography	758:802	enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies	741:827	Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies.
24679409	5	56	theme	hydrophilic	758:768	arg1	HILIC					805:809	HILIC	805:809	HILIC	805:809	Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies.
24679409	7	57	theme	1mLmin	1048:1053	arg1	rate					1040:1043	a flow rate	1033:1043	a flow rate of 1mLmin(-1)	1033:1057	At a flow rate of 1mLmin(-1) the separation was completed within 8min.
24679409	8	58	theme	0.21μgmL	1207:1214	arg1	impurity					1186:1193	the enantiomeric impurity	1169:1193	the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively	1169:1232	The optimized method was proven to be sensitive with LOD and LOQ of the enantiomeric impurity of 0.07 and 0.21μgmL(-1), respectively.
24679409	0	59	theme	cellulose-based	57:71	arg1	phase					91:95	a cellulose-based chiral stationary phase	55:95	a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode	55:149	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	0	60	from	phase	91:95	arg1	mode					146:149	hydrophilic interaction liquid chromatography mode	100:149	hydrophilic interaction liquid chromatography mode	100:149	Direct separation of the enantiomers of oxaliplatin on a cellulose-based chiral stationary phase in hydrophilic interaction liquid chromatography mode.
24679409	5	61	theme	chromatography	789:802	arg1	strategies					818:827	enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies	741:827	enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies	741:827	Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies.
24679409	4	62	dep	generation	533:542	arg1	CSP					590:592	CSP	590:592	CSP	590:592	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	4	62	dep	generation	533:542	arg1	phase					583:587	polysaccharide-based chiral stationary phase	544:587	polysaccharide-based chiral stationary phase (CSP)	544:593	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
24679409	5	63	theme	based	812:816	arg1	strategies					818:827	enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies	741:827	enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies	741:827	Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies.
24679409	4	64	theme	Chiralpak	605:613	arg1	IC-3					615:618	the Chiralpak IC-3	601:618	the Chiralpak IC-3	601:618	In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin.
25422061	6	0	located	found	977:981	arg1	cellulose					986:994	cellulose	986:994	cellulose	986:994	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	0	located	found	977:981	arg2	groups					960:965	clear functional groups	943:965	clear functional groups that were found in cellulose	943:994	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	1	theme	high	1012:1015	arg1	crystallinity					1017:1029	relatively high crystallinity	1001:1029	relatively high crystallinity	1001:1029	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	2	theme	nano-network	919:930	arg1	structure					932:940	obvious nano-network structure	911:940	obvious nano-network structure	911:940	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	0	3	theme	Gluconacetobacter	78:94	arg1	xylinus					96:102	Gluconacetobacter xylinus	78:102	Gluconacetobacter xylinus	78:102	Utilization of corncob acid hydrolysate for bacterial cellulose production by Gluconacetobacter xylinus.
25422061	6	4	theme	scanning	766:773	arg1	FE-SEM					796:801	FE-SEM	796:801	FE-SEM	796:801	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	4	theme	scanning	766:773	arg1	microscope					784:793	scanning electron microscope	766:793	field emission scanning electron microscope (FE-SEM)	751:802	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	5	theme	obvious	911:917	arg1	structure					932:940	obvious nano-network structure	911:940	obvious nano-network structure	911:940	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	5	6	theme	inoculation	616:626	arg1	concentration					628:640	inoculation concentration	616:640	inoculation concentration	616:640	Fermentation condition including fermentation mode, inoculation concentration, and initial pH showed certain influence on the BC yield and thus should be well controlled.
25422061	5	7	theme	BC	690:691	arg1	yield					693:697	the BC yield	686:697	the BC yield	686:697	Fermentation condition including fermentation mode, inoculation concentration, and initial pH showed certain influence on the BC yield and thus should be well controlled.
25422061	6	8	theme	field	751:755	arg1	emission					757:764	field emission scanning electron microscope (FE-SEM)	751:802	field emission scanning electron microscope (FE-SEM)	751:802	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	9	theme	crystallinity	1035:1047	arg1	value					1055:1059	crystallinity index value	1035:1059	crystallinity index value	1035:1059	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	4	10	theme	carbon	436:441	arg1	sources					456:462	carbon and nitrogen sources	436:462	sources	456:462	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
25422061	4	10	theme	carbon	436:441	arg1	substrates					424:433	Most extra substrates	413:433	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid	413:513	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
25422061	5	11	theme	Fermentation	564:575	arg1	condition					577:585	Fermentation condition	564:585	Fermentation condition including fermentation mode, inoculation concentration, and initial pH	564:656	Fermentation condition including fermentation mode, inoculation concentration, and initial pH showed certain influence on the BC yield and thus should be well controlled.
25422061	1	12	used	used	149:152	arg2	substrate					159:167	a substrate	157:167	a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus	157:236	In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus.
25422061	1	12	used	used	149:152	arg2	hydrolysate					133:143	corncob acid hydrolysate	120:143	corncob acid hydrolysate	120:143	In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus.
25422061	6	13	theme	index	1049:1053	arg1	value					1055:1059	crystallinity index value	1035:1059	crystallinity index value	1035:1059	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	7	14	contain	had	1086:1088	arg2	capacity					1110:1117	great water-holding capacity	1090:1117	great water-holding capacity	1090:1117	Moreover, the BC sample had great water-holding capacity.
25422061	7	14	contain	had	1086:1088	arg1	sample					1079:1084	the BC sample	1072:1084	the BC sample	1072:1084	Moreover, the BC sample had great water-holding capacity.
25422061	2	15	theme	static	254:259	arg1	fermentation					261:272	2 weeks' static fermentation	245:272	2 weeks' static fermentation	245:272	After 2 weeks' static fermentation, a BC yield of 4 g/L could be obtained.
25422061	7	16	theme	great	1090:1094	arg1	capacity					1110:1117	great water-holding capacity	1090:1117	great water-holding capacity	1090:1117	Moreover, the BC sample had great water-holding capacity.
25422061	4	17	theme	nitrogen	447:454	arg1	sources					456:462	carbon and nitrogen sources	436:462	sources	456:462	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
25422061	4	17	theme	nitrogen	447:454	arg1	substrates					424:433	Most extra substrates	413:433	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid	413:513	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
25422061	8	18	theme	promising	1167:1175	arg1	substrate					1177:1185	one promising substrate	1163:1185	one promising substrate for BC production	1163:1203	Overall, corncob acid hydrolysate could be one promising substrate for BC production.
25422061	1	19	theme	bacterial	173:181	arg1	production					198:207	bacterial cellulose (BC) production	173:207	bacterial cellulose (BC) production by Gluconacetobacter xylinus	173:236	In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus.
25422061	3	20	theme	fermentation	353:364	arg1	condition					366:374	fermentation condition	353:374	fermentation condition	353:374	Both effects of medium composition and fermentation condition on the BC production were evaluated.
25422061	6	21	theme	BC	897:898	arg1	sample					900:905	the BC sample	893:905	the BC sample	893:905	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	5	22	theme	fermentation	597:608	arg1	mode					610:613	fermentation mode	597:613	fermentation mode	597:613	Fermentation condition including fermentation mode, inoculation concentration, and initial pH showed certain influence on the BC yield and thus should be well controlled.
25422061	6	23	contain	had	907:909	arg2	groups					960:965	clear functional groups	943:965	clear functional groups that were found in cellulose	943:994	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	23	contain	had	907:909	arg1	sample					900:905	the BC sample	893:905	the BC sample	893:905	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	23	contain	had	907:909	arg2	structure					932:940	obvious nano-network structure	911:940	obvious nano-network structure	911:940	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	23	contain	had	907:909	arg2	crystallinity					1017:1029	relatively high crystallinity	1001:1029	relatively high crystallinity	1001:1029	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	1	24	theme	cellulose	183:191	arg1	production					198:207	bacterial cellulose (BC) production	173:207	bacterial cellulose (BC) production by Gluconacetobacter xylinus	173:236	In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus.
25422061	0	25	theme	acid	23:26	arg1	hydrolysate					28:38	corncob acid hydrolysate	15:38	corncob acid hydrolysate	15:38	Utilization of corncob acid hydrolysate for bacterial cellulose production by Gluconacetobacter xylinus.
25422061	4	26	theme	levulinic	500:508	arg1	acid					510:513	levulinic acid	500:513	levulinic acid	500:513	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
25422061	8	27	theme	BC	1191:1192	arg1	production					1194:1203	BC production	1191:1203	BC production	1191:1203	Overall, corncob acid hydrolysate could be one promising substrate for BC production.
25422061	3	28	theme	composition	337:347	arg1	effects					319:325	Both effects	314:325	Both effects of medium composition and fermentation condition on the BC production	314:395	Both effects of medium composition and fermentation condition on the BC production were evaluated.
25422061	5	29	theme	certain	665:671	arg1	influence					673:681	certain influence	665:681	certain influence	665:681	Fermentation condition including fermentation mode, inoculation concentration, and initial pH showed certain influence on the BC yield and thus should be well controlled.
25422061	4	30	theme	extra	418:422	arg1	sources					456:462	carbon and nitrogen sources	436:462	sources	456:462	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
25422061	4	30	theme	extra	418:422	arg1	substrates					424:433	Most extra substrates	413:433	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid	413:513	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
25422061	2	31	theme	BC	277:278	arg1	yield					280:284	a BC yield	275:284	a BC yield of 4 g/L	275:293	After 2 weeks' static fermentation, a BC yield of 4 g/L could be obtained.
25422061	7	32	theme	BC	1076:1077	arg1	sample					1079:1084	the BC sample	1072:1084	the BC sample	1072:1084	Moreover, the BC sample had great water-holding capacity.
25422061	8	33	theme	acid	1137:1140	arg1	hydrolysate					1142:1152	corncob acid hydrolysate	1129:1152	corncob acid hydrolysate	1129:1152	Overall, corncob acid hydrolysate could be one promising substrate for BC production.
25422061	4	34	theme	Most	413:416	arg1	sources					456:462	carbon and nitrogen sources	436:462	sources	456:462	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
25422061	4	34	theme	Most	413:416	arg1	substrates					424:433	Most extra substrates	413:433	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid	413:513	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
25422061	6	35	dep	emission	757:764	arg1	FE-SEM					796:801	FE-SEM	796:801	FE-SEM	796:801	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	35	dep	emission	757:764	arg1	microscope					784:793	scanning electron microscope	766:793	field emission scanning electron microscope (FE-SEM)	751:802	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	0	36	theme	hydrolysate	28:38	arg1	Utilization					0:10	Utilization	0:10	Utilization of corncob acid hydrolysate for bacterial cellulose production by Gluconacetobacter xylinus.	0:103	Utilization of corncob acid hydrolysate for bacterial cellulose production by Gluconacetobacter xylinus.
25422061	3	37	theme	BC	383:384	arg1	production					386:395	the BC production	379:395	the BC production	379:395	Both effects of medium composition and fermentation condition on the BC production were evaluated.
25422061	3	38	from	effects	319:325	arg1	production					386:395	the BC production	379:395	the BC production	379:395	Both effects of medium composition and fermentation condition on the BC production were evaluated.
25422061	3	39	theme	medium	330:335	arg1	composition					337:347	medium composition	330:347	medium composition	330:347	Both effects of medium composition and fermentation condition on the BC production were evaluated.
25422061	0	40	theme	cellulose	54:62	arg1	production					64:73	bacterial cellulose production	44:73	bacterial cellulose production by Gluconacetobacter xylinus	44:102	Utilization of corncob acid hydrolysate for bacterial cellulose production by Gluconacetobacter xylinus.
25422061	4	41	theme	butyric	482:488	arg1	acid					490:493	butyric acid	482:493	butyric acid	482:493	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
25422061	8	42	theme	corncob	1129:1135	arg1	hydrolysate					1142:1152	corncob acid hydrolysate	1129:1152	corncob acid hydrolysate	1129:1152	Overall, corncob acid hydrolysate could be one promising substrate for BC production.
25422061	0	43	theme	bacterial	44:52	arg1	production					64:73	bacterial cellulose production	44:73	bacterial cellulose production by Gluconacetobacter xylinus	44:102	Utilization of corncob acid hydrolysate for bacterial cellulose production by Gluconacetobacter xylinus.
25422061	6	44	dep	transform	813:821	arg1	infrared					823:830	infrared	823:830	transform infrared spectroscopy (FTIR)	813:850	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	1	45	theme	corncob	120:126	arg1	substrate					159:167	a substrate	157:167	a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus	157:236	In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus.
25422061	1	45	theme	corncob	120:126	arg1	hydrolysate					133:143	corncob acid hydrolysate	120:143	corncob acid hydrolysate	120:143	In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus.
25422061	6	46	dep	Fourier	805:811	arg1	transform					813:821	transform	813:821	transform infrared spectroscopy (FTIR)	813:850	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	5	47	theme	initial	647:653	arg1	pH					655:656	initial pH	647:656	initial pH	647:656	Fermentation condition including fermentation mode, inoculation concentration, and initial pH showed certain influence on the BC yield and thus should be well controlled.
25422061	6	48	theme	clear	943:947	arg1	groups					960:965	clear functional groups	943:965	clear functional groups that were found in cellulose	943:994	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	1	49	theme	acid	128:131	arg1	substrate					159:167	a substrate	157:167	a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus	157:236	In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus.
25422061	1	49	theme	acid	128:131	arg1	hydrolysate					133:143	corncob acid hydrolysate	120:143	corncob acid hydrolysate	120:143	In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus.
25422061	4	50	theme	BC	554:555	arg1	yield					557:561	BC yield	554:561	BC yield	554:561	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
25422061	2	51	theme	g/L	291:293	arg1	yield					280:284	a BC yield	275:284	a BC yield of 4 g/L	275:293	After 2 weeks' static fermentation, a BC yield of 4 g/L could be obtained.
25422061	1	52	theme	Gluconacetobacter	212:228	arg1	xylinus					230:236	Gluconacetobacter xylinus	212:236	Gluconacetobacter xylinus	212:236	In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus.
25422061	6	53	theme	electron	775:782	arg1	FE-SEM					796:801	FE-SEM	796:801	FE-SEM	796:801	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	53	theme	electron	775:782	arg1	microscope					784:793	scanning electron microscope	766:793	field emission scanning electron microscope (FE-SEM)	751:802	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	3	54	theme	condition	366:374	arg1	effects					319:325	Both effects	314:325	Both effects of medium composition and fermentation condition on the BC production	314:395	Both effects of medium composition and fermentation condition on the BC production were evaluated.
25422061	6	55	theme	X-ray	857:861	arg1	XRD					876:878	XRD	876:878	XRD	876:878	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	6	55	theme	X-ray	857:861	arg1	diffraction					863:873	X-ray diffraction	857:873	X-ray diffraction (XRD)	857:879	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	0	56	theme	corncob	15:21	arg1	hydrolysate					28:38	corncob acid hydrolysate	15:38	corncob acid hydrolysate	15:38	Utilization of corncob acid hydrolysate for bacterial cellulose production by Gluconacetobacter xylinus.
25422061	6	57	theme	functional	949:958	arg1	groups					960:965	clear functional groups	943:965	clear functional groups that were found in cellulose	943:994	The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value.
25422061	7	58	theme	water-holding	1096:1108	arg1	capacity					1110:1117	great water-holding capacity	1090:1117	great water-holding capacity	1090:1117	Moreover, the BC sample had great water-holding capacity.
25422061	4	59	theme	yield	557:561	arg1	improvement					539:549	the improvement	535:549	the improvement of BC yield	535:561	Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield.
23909466	8	0	theme	Long-chain	1187:1196	arg1	inulin					1198:1203	Long-chain inulin	1187:1203	Long-chain inulin	1187:1203	Long-chain inulin was utilised by Roseburia inulinivorans, but by none of the Bifidobacterium species examined here.
23909466	1	1	theme	Dietary	97:103	arg1	macronutrients					105:118	Dietary macronutrients	97:118	Dietary macronutrients	97:118	Dietary macronutrients affect the composition of the gut microbiota, and prebiotics are used to improve and maintain a healthy gut.
23909466	10	2	theme	different	1471:1479	arg1	prebiotics					1481:1490	different prebiotics	1471:1490	different prebiotics	1471:1490	These results illustrate the selectivity of different prebiotics and help to explain why some are butyrogenic.
23909466	9	3	with	substrate	1336:1344	arg1	strains					1391:1397	the 11 Firmicutes strains	1373:1397	the 11 Firmicutes strains able to use XOS for growth	1373:1424	XOS was a more selective growth substrate than FOS, with only six of the 11 Firmicutes strains able to use XOS for growth.
23909466	9	3	with	substrate	1336:1344	arg1	six					1366:1368	six	1366:1368	six	1366:1368	XOS was a more selective growth substrate than FOS, with only six of the 11 Firmicutes strains able to use XOS for growth.
23909466	0	4	theme	in	87:88	arg1	bifidobacteria					71:84	bifidobacteria	71:84	bifidobacteria	71:84	Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro.
23909466	9	5	theme	growth	1329:1334	arg1	XOS					1304:1306	XOS	1304:1306	XOS	1304:1306	XOS was a more selective growth substrate than FOS, with only six of the 11 Firmicutes strains able to use XOS for growth.
23909466	9	5	theme	growth	1329:1334	arg1	substrate					1336:1344	a more selective growth substrate	1312:1344	a more selective growth substrate than FOS	1312:1353	XOS was a more selective growth substrate than FOS, with only six of the 11 Firmicutes strains able to use XOS for growth.
23909466	7	6	theme	fructan	1157:1163	arg1	substrates					1165:1174	the fructan substrates	1153:1174	the fructan substrates	1153:1174	Eleven were able to grow on short-chain FOS, but this number decreased as the chain length of the fructan substrates increased.
23909466	3	7	theme	selected	542:549	arg1	Bacteroides					551:561	selected Bacteroides and Bifidobacterium species	542:589	Bacteroides	551:561	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	1	8	used	used	185:188	arg2	prebiotics					170:179	prebiotics	170:179	prebiotics	170:179	Dietary macronutrients affect the composition of the gut microbiota, and prebiotics are used to improve and maintain a healthy gut.
23909466	2	9	theme	other	279:283	arg1	bacteria					270:277	dominant gut bacteria	257:277	dominant gut bacteria other than bifidobacteria	257:303	The impact of prebiotics on dominant gut bacteria other than bifidobacteria, however, is under-researched.
23909466	4	10	theme	new	647:649	arg1	assay					674:678	a new rapid microtitre plate assay	645:678	a new rapid microtitre plate assay	645:678	Growth assessments using anaerobic Hungate tubes and a new rapid microtitre plate assay were generally in good agreement.
23909466	5	11	theme	Bacteroides	718:728	arg1	strains					730:736	The Bacteroides strains	714:736	The Bacteroides strains tested	714:743	The Bacteroides strains tested showed some growth on basal medium with no added carbohydrates, utilising peptides in the growth medium.
23909466	6	12	theme	butyrate-producing	854:871	arg1	strains					873:879	The butyrate-producing strains	850:879	The butyrate-producing strains	850:879	The butyrate-producing strains exhibited different growth profiles on the substrates, which included starch, inulin, fructooligosaccharides (FOS), galactooligosaccharides (GOS) and xylooligosaccharides (XOS).
23909466	5	13	theme	added	788:792	arg1	carbohydrates					794:806	no added carbohydrates	785:806	no added carbohydrates	785:806	The Bacteroides strains tested showed some growth on basal medium with no added carbohydrates, utilising peptides in the growth medium.
23909466	2	14	theme	gut	266:268	arg1	bacteria					270:277	dominant gut bacteria	257:277	dominant gut bacteria other than bifidobacteria	257:303	The impact of prebiotics on dominant gut bacteria other than bifidobacteria, however, is under-researched.
23909466	8	15	theme	species	1281:1287	arg1	none					1253:1256	none	1253:1256	none of the Bifidobacterium species examined here	1253:1301	Long-chain inulin was utilised by Roseburia inulinivorans, but by none of the Bifidobacterium species examined here.
23909466	3	16	theme	butyrate-producing	405:422	arg1	anaerobes					424:432	representative butyrate-producing anaerobes	390:432	representative butyrate-producing anaerobes	390:432	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	7	17	theme	chain	1137:1141	arg1	length					1143:1148	the chain length	1133:1148	the chain length of the fructan substrates	1133:1174	Eleven were able to grow on short-chain FOS, but this number decreased as the chain length of the fructan substrates increased.
23909466	7	18	theme	substrates	1165:1174	arg1	length					1143:1148	the chain length	1133:1148	the chain length of the fructan substrates	1133:1174	Eleven were able to grow on short-chain FOS, but this number decreased as the chain length of the fructan substrates increased.
23909466	2	19	theme	dominant	257:264	arg1	bacteria					270:277	dominant gut bacteria	257:277	dominant gut bacteria other than bifidobacteria	257:303	The impact of prebiotics on dominant gut bacteria other than bifidobacteria, however, is under-researched.
23909466	4	20	theme	microtitre	657:666	arg1	assay					674:678	a new rapid microtitre plate assay	645:678	a new rapid microtitre plate assay	645:678	Growth assessments using anaerobic Hungate tubes and a new rapid microtitre plate assay were generally in good agreement.
23909466	3	21	theme	Gram-positive	452:464	arg1	families					477:484	the Gram-positive Firmicutes families	448:484	the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae	448:520	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	3	21	theme	Gram-positive	452:464	arg1	Ruminococcaceae					506:520	Ruminococcaceae	506:520	Ruminococcaceae	506:520	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	3	21	theme	Gram-positive	452:464	arg1	Lachnospiraceae					486:500	Lachnospiraceae	486:500	Lachnospiraceae	486:500	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	8	22	theme	Roseburia	1221:1229	arg1	inulinivorans					1231:1243	Roseburia inulinivorans	1221:1243	Roseburia inulinivorans	1221:1243	Long-chain inulin was utilised by Roseburia inulinivorans, but by none of the Bifidobacterium species examined here.
23909466	10	23	theme	prebiotics	1481:1490	arg1	selectivity					1456:1466	the selectivity	1452:1466	the selectivity of different prebiotics	1452:1490	These results illustrate the selectivity of different prebiotics and help to explain why some are butyrogenic.
23909466	0	24	theme	Prebiotic	0:8	arg1	stimulation					10:20	Prebiotic stimulation	0:20	Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro.	0:95	Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro.
23909466	3	25	with	comparison	526:535	arg1	Bacteroides					551:561	selected Bacteroides and Bifidobacterium species	542:589	Bacteroides	551:561	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	3	25	with	comparison	526:535	arg1	species					583:589	selected Bacteroides and Bifidobacterium species	542:589	species	583:589	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	9	26	theme	strains	1391:1397	arg1	strains					1391:1397	the 11 Firmicutes strains	1373:1397	the 11 Firmicutes strains able to use XOS for growth	1373:1424	XOS was a more selective growth substrate than FOS, with only six of the 11 Firmicutes strains able to use XOS for growth.
23909466	9	26	theme	strains	1391:1397	arg1	six					1366:1368	six	1366:1368	six	1366:1368	XOS was a more selective growth substrate than FOS, with only six of the 11 Firmicutes strains able to use XOS for growth.
23909466	0	27	dep	in	87:88	arg1	vitro					90:94	vitro	90:94	vitro	90:94	Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro.
23909466	3	28	theme	carbohydrate	352:363	arg1	patterns					377:384	carbohydrate utilisation patterns	352:384	carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae,	352:521	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	2	29	from	impact	233:238	arg1	bacteria					270:277	dominant gut bacteria	257:277	dominant gut bacteria other than bifidobacteria	257:303	The impact of prebiotics on dominant gut bacteria other than bifidobacteria, however, is under-researched.
23909466	3	30	theme	utilisation	365:375	arg1	patterns					377:384	carbohydrate utilisation patterns	352:384	carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae,	352:521	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	4	31	theme	Hungate	627:633	arg1	tubes					635:639	anaerobic Hungate tubes	617:639	anaerobic Hungate tubes	617:639	Growth assessments using anaerobic Hungate tubes and a new rapid microtitre plate assay were generally in good agreement.
23909466	3	32	theme	Bifidobacterium	567:581	arg1	species					583:589	selected Bacteroides and Bifidobacterium species	542:589	species	583:589	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	5	33	with	medium	773:778	arg1	carbohydrates					794:806	no added carbohydrates	785:806	no added carbohydrates	785:806	The Bacteroides strains tested showed some growth on basal medium with no added carbohydrates, utilising peptides in the growth medium.
23909466	0	34	theme	butyrate-producing	39:56	arg1	bacteria					58:65	human colonic butyrate-producing bacteria	25:65	human colonic butyrate-producing bacteria	25:65	Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro.
23909466	4	35	theme	anaerobic	617:625	arg1	tubes					635:639	anaerobic Hungate tubes	617:639	anaerobic Hungate tubes	617:639	Growth assessments using anaerobic Hungate tubes and a new rapid microtitre plate assay were generally in good agreement.
23909466	5	36	theme	basal	767:771	arg1	medium					773:778	basal medium	767:778	basal medium with no added carbohydrates	767:806	The Bacteroides strains tested showed some growth on basal medium with no added carbohydrates, utilising peptides in the growth medium.
23909466	4	37	theme	good	698:701	arg1	agreement					703:711	good agreement	698:711	good agreement	698:711	Growth assessments using anaerobic Hungate tubes and a new rapid microtitre plate assay were generally in good agreement.
23909466	3	38	theme	Firmicutes	466:475	arg1	families					477:484	the Gram-positive Firmicutes families	448:484	the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae	448:520	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	3	38	theme	Firmicutes	466:475	arg1	Ruminococcaceae					506:520	Ruminococcaceae	506:520	Ruminococcaceae	506:520	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	3	38	theme	Firmicutes	466:475	arg1	Lachnospiraceae					486:500	Lachnospiraceae	486:500	Lachnospiraceae	486:500	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	0	39	theme	colonic	31:37	arg1	bacteria					58:65	human colonic butyrate-producing bacteria	25:65	human colonic butyrate-producing bacteria	25:65	Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro.
23909466	5	40	theme	growth	835:840	arg1	medium					842:847	the growth medium	831:847	the growth medium	831:847	The Bacteroides strains tested showed some growth on basal medium with no added carbohydrates, utilising peptides in the growth medium.
23909466	0	41	theme	human	25:29	arg1	bacteria					58:65	human colonic butyrate-producing bacteria	25:65	human colonic butyrate-producing bacteria	25:65	Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro.
23909466	6	42	from	profiles	908:915	arg1	substrates					924:933	the substrates	920:933	the substrates	920:933	The butyrate-producing strains exhibited different growth profiles on the substrates, which included starch, inulin, fructooligosaccharides (FOS), galactooligosaccharides (GOS) and xylooligosaccharides (XOS).
23909466	3	43	theme	representative	390:403	arg1	anaerobes					424:432	representative butyrate-producing anaerobes	390:432	representative butyrate-producing anaerobes	390:432	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	9	44	theme	Firmicutes	1380:1389	arg1	strains					1391:1397	the 11 Firmicutes strains	1373:1397	the 11 Firmicutes strains able to use XOS for growth	1373:1424	XOS was a more selective growth substrate than FOS, with only six of the 11 Firmicutes strains able to use XOS for growth.
23909466	7	45	theme	short-chain	1087:1097	arg1	FOS					1099:1101	short-chain FOS	1087:1101	short-chain FOS	1087:1101	Eleven were able to grow on short-chain FOS, but this number decreased as the chain length of the fructan substrates increased.
23909466	0	46	theme	bacteria	58:65	arg1	stimulation					10:20	Prebiotic stimulation	0:20	Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro.	0:95	Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro.
23909466	4	47	theme	Growth	592:597	arg1	assessments					599:609	Growth assessments	592:609	Growth assessments using anaerobic Hungate tubes	592:639	Growth assessments using anaerobic Hungate tubes and a new rapid microtitre plate assay were generally in good agreement.
23909466	5	48	from	peptides	819:826	arg1	medium					842:847	the growth medium	831:847	the growth medium	831:847	The Bacteroides strains tested showed some growth on basal medium with no added carbohydrates, utilising peptides in the growth medium.
23909466	9	49	theme	selective	1319:1327	arg1	XOS					1304:1306	XOS	1304:1306	XOS	1304:1306	XOS was a more selective growth substrate than FOS, with only six of the 11 Firmicutes strains able to use XOS for growth.
23909466	9	49	theme	selective	1319:1327	arg1	substrate					1336:1344	a more selective growth substrate	1312:1344	a more selective growth substrate than FOS	1312:1353	XOS was a more selective growth substrate than FOS, with only six of the 11 Firmicutes strains able to use XOS for growth.
23909466	9	50	theme	able	1399:1402	arg1	strains					1391:1397	the 11 Firmicutes strains	1373:1397	the 11 Firmicutes strains able to use XOS for growth	1373:1424	XOS was a more selective growth substrate than FOS, with only six of the 11 Firmicutes strains able to use XOS for growth.
23909466	1	51	theme	healthy	216:222	arg1	gut					224:226	a healthy gut	214:226	a healthy gut	214:226	Dietary macronutrients affect the composition of the gut microbiota, and prebiotics are used to improve and maintain a healthy gut.
23909466	3	52	dep	families	477:484	arg1	families					477:484	the Gram-positive Firmicutes families	448:484	the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae	448:520	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	3	52	dep	families	477:484	arg1	Ruminococcaceae					506:520	Ruminococcaceae	506:520	Ruminococcaceae	506:520	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	3	52	dep	families	477:484	arg1	Lachnospiraceae					486:500	Lachnospiraceae	486:500	Lachnospiraceae	486:500	Here, we report carbohydrate utilisation patterns for representative butyrate-producing anaerobes, belonging to the Gram-positive Firmicutes families Lachnospiraceae and Ruminococcaceae, by comparison with selected Bacteroides and Bifidobacterium species.
23909466	6	53	theme	growth	901:906	arg1	profiles					908:915	different growth profiles	891:915	different growth profiles on the substrates, which included starch, inulin, fructooligosaccharides (FOS), galactooligosaccharides (GOS) and xylooligosaccharides (XOS)	891:1056	The butyrate-producing strains exhibited different growth profiles on the substrates, which included starch, inulin, fructooligosaccharides (FOS), galactooligosaccharides (GOS) and xylooligosaccharides (XOS).
23909466	2	54	theme	prebiotics	243:252	arg1	impact					233:238	The impact	229:238	The impact of prebiotics on dominant gut bacteria other than bifidobacteria	229:303	The impact of prebiotics on dominant gut bacteria other than bifidobacteria, however, is under-researched.
23909466	4	55	theme	rapid	651:655	arg1	assay					674:678	a new rapid microtitre plate assay	645:678	a new rapid microtitre plate assay	645:678	Growth assessments using anaerobic Hungate tubes and a new rapid microtitre plate assay were generally in good agreement.
23909466	6	56	theme	different	891:899	arg1	profiles					908:915	different growth profiles	891:915	different growth profiles on the substrates, which included starch, inulin, fructooligosaccharides (FOS), galactooligosaccharides (GOS) and xylooligosaccharides (XOS)	891:1056	The butyrate-producing strains exhibited different growth profiles on the substrates, which included starch, inulin, fructooligosaccharides (FOS), galactooligosaccharides (GOS) and xylooligosaccharides (XOS).
23909466	1	57	theme	gut	150:152	arg1	microbiota					154:163	the gut microbiota	146:163	the gut microbiota	146:163	Dietary macronutrients affect the composition of the gut microbiota, and prebiotics are used to improve and maintain a healthy gut.
23909466	8	58	theme	Bifidobacterium	1265:1279	arg1	species					1281:1287	the Bifidobacterium species	1261:1287	the Bifidobacterium species examined here	1261:1301	Long-chain inulin was utilised by Roseburia inulinivorans, but by none of the Bifidobacterium species examined here.
23909466	0	59	theme	bifidobacteria	71:84	arg1	stimulation					10:20	Prebiotic stimulation	0:20	Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro.	0:95	Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro.
23909466	1	60	theme	microbiota	154:163	arg1	composition					131:141	the composition	127:141	the composition of the gut microbiota	127:163	Dietary macronutrients affect the composition of the gut microbiota, and prebiotics are used to improve and maintain a healthy gut.
23909466	4	61	theme	plate	668:672	arg1	assay					674:678	a new rapid microtitre plate assay	645:678	a new rapid microtitre plate assay	645:678	Growth assessments using anaerobic Hungate tubes and a new rapid microtitre plate assay were generally in good agreement.
26492524	0	0	from	augmentation	11:22	arg1	bone					43:46	mouse calvarial bone	27:46	mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma	27:138	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
26492524	8	1	dep	CONCLUSIONS	1192:1202	arg1	valid					1255:1259	valid	1255:1259	valid	1255:1259	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	8	1	dep	CONCLUSIONS	1192:1202	arg1	material					1242:1249	The hydroxyapatite/collagen composite material	1204:1249	The hydroxyapatite/collagen composite material	1204:1249	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	8	1	dep	CONCLUSIONS	1192:1202	arg1	soaked					1317:1322	soaked	1317:1322	should be soaked in total blood or platelet-rich plasma prior to transplantation	1307:1386	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	6	2	located	observed	908:915	arg1	materials					962:970	the transplanted materials	945:970	the transplanted materials with total blood or platelet-rich plasma but not with normal saline	945:1038	Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline.
26492524	6	2	located	observed	908:915	arg2	marrow					897:902	bone marrow	892:902	bone marrow	892:902	Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline.
26492524	0	3	theme	composite	80:88	arg1	material					90:97	a hydroxyapatite/collagen composite material	54:97	a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma	54:138	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
26492524	0	4	with	material	90:97	arg1	blood					110:114	total blood	104:114	total blood	104:114	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
26492524	0	4	with	material	90:97	arg1	plasma					133:138	platelet-rich plasma	119:138	platelet-rich plasma	119:138	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
26492524	8	5	theme	prior	1363:1367	arg1	blood					1333:1337	total blood	1327:1337	total blood	1327:1337	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	6	6	dep	weeks	922:926	arg1	surgery					934:940	surgery	934:940	surgery	934:940	Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline.
26492524	0	7	theme	total	104:108	arg1	blood					110:114	total blood	104:114	total blood	104:114	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
26492524	1	8	theme	platelet-rich	330:342	arg1	plasma					344:349	platelet-rich plasma	330:349	platelet-rich plasma	330:349	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	7	9	theme	bone	1118:1121	arg1	amount					1095:1100	the amount	1091:1100	the amount of newly formed bone	1091:1121	However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma.
26492524	7	9	theme	bone	1118:1121	arg1	bone					1118:1121	newly formed bone	1105:1121	newly formed bone	1105:1121	However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma.
26492524	6	10	theme	platelet-rich	992:1004	arg1	plasma					1006:1011	platelet-rich plasma	992:1011	platelet-rich plasma	992:1011	Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline.
26492524	1	11	theme	new	243:245	arg1	composite					299:307	a hydroxyapatite/collagen composite	273:307	a hydroxyapatite/collagen composite	273:307	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	1	11	theme	new	243:245	arg1	material					263:270	a new artificial bone material	241:270	a new artificial bone material	241:270	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	5	12	theme	total	756:760	arg1	blood					762:766	total blood	756:766	total blood	756:766	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
26492524	1	13	dep	OBJECTIVE	141:149	arg1	was					173:175	was	173:175	was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma	173:349	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	1	14	theme	artificial	247:256	arg1	composite					299:307	a hydroxyapatite/collagen composite	273:307	a hydroxyapatite/collagen composite	273:307	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	1	14	theme	artificial	247:256	arg1	material					263:270	a new artificial bone material	241:270	a new artificial bone material	241:270	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	5	15	theme	normal	867:872	arg1	saline					874:879	normal saline	867:879	normal saline	867:879	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
26492524	5	16	with	materials	741:749	arg1	blood					762:766	total blood	756:766	total blood	756:766	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
26492524	5	16	with	materials	741:749	arg1	plasma					785:790	platelet-rich plasma	771:790	platelet-rich plasma	771:790	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
26492524	4	17	theme	Image	677:681	arg1	software					685:692	Image J software	677:692	Image J software	677:692	The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software.
26492524	1	18	theme	study	167:171	arg1	aim					155:157	The aim	151:157	The aim of this study	151:171	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	7	19	theme	total	1151:1155	arg1	blood					1157:1161	total blood	1151:1161	total blood	1151:1161	However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma.
26492524	2	20	theme	mouse	482:486	arg1	bone					498:501	mouse calvarial bone	482:501	mouse calvarial bone	482:501	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone.
26492524	2	21	theme	platelet-rich	441:453	arg1	plasma					455:460	platelet-rich plasma	441:460	platelet-rich plasma	441:460	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone.
26492524	4	22	theme	stained	653:659	arg1	specimens					661:669	hematoxylin and eosin stained specimens	631:669	hematoxylin and eosin stained specimens using Image J software	631:692	The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software.
26492524	0	23	theme	bone	6:9	arg1	augmentation					11:22	Onlay bone augmentation	0:22	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.	0:139	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
26492524	2	24	theme	calvarial	488:496	arg1	bone					498:501	mouse calvarial bone	482:501	mouse calvarial bone	482:501	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone.
26492524	5	25	theme	platelet-rich	771:783	arg1	plasma					785:790	platelet-rich plasma	771:790	platelet-rich plasma	771:790	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
26492524	2	26	theme	composite	387:395	arg1	material					397:404	The hydroxyapatite/collagen composite material	359:404	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma	352:460	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone.
26492524	0	27	theme	Onlay	0:4	arg1	augmentation					11:22	Onlay bone augmentation	0:22	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.	0:139	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
26492524	1	28	theme	bone	258:261	arg1	composite					299:307	a hydroxyapatite/collagen composite	273:307	a hydroxyapatite/collagen composite	273:307	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	1	28	theme	bone	258:261	arg1	material					263:270	a new artificial bone material	241:270	a new artificial bone material	241:270	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	2	29	theme	hydroxyapatite/collagen	363:385	arg1	material					397:404	The hydroxyapatite/collagen composite material	359:404	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma	352:460	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone.
26492524	4	30	theme	bone	605:608	arg1	area					610:613	The newly formed bone area	588:613	The newly formed bone area	588:613	The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software.
26492524	5	31	theme	formed	840:845	arg1	bone					847:850	newly formed bone	834:850	newly formed bone than that with normal saline	834:879	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
26492524	2	32	theme	total	426:430	arg1	blood					432:436	total blood	426:436	total blood	426:436	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone.
26492524	8	33	theme	composite	1232:1240	arg1	valid					1255:1259	valid	1255:1259	valid	1255:1259	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	8	33	theme	composite	1232:1240	arg1	material					1242:1249	The hydroxyapatite/collagen composite material	1204:1249	The hydroxyapatite/collagen composite material	1204:1249	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	7	34	theme	significant	1064:1074	arg1	differences					1076:1086	no significant differences	1061:1086	no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma	1061:1189	However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma.
26492524	0	35	theme	platelet-rich	119:131	arg1	plasma					133:138	platelet-rich plasma	119:138	platelet-rich plasma	119:138	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
26492524	6	36	theme	bone	892:895	arg1	marrow					897:902	bone marrow	892:902	bone marrow	892:902	Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline.
26492524	6	37	with	materials	962:970	arg1	blood					983:987	total blood	977:987	total blood	977:987	Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline.
26492524	6	37	with	materials	962:970	arg1	saline					1033:1038	normal saline	1026:1038	normal saline	1026:1038	Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline.
26492524	1	38	theme	formed	193:198	arg1	bone					206:209	newly formed onlay bone	187:209	newly formed onlay bone	187:209	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	0	39	theme	calvarial	33:41	arg1	bone					43:46	mouse calvarial bone	27:46	mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma	27:138	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
26492524	6	40	theme	total	977:981	arg1	blood					983:987	total blood	977:987	total blood	977:987	Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline.
26492524	5	41	theme	composite	731:739	arg1	materials					741:749	The hydroxyapatite/collagen composite materials	703:749	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma	695:790	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
26492524	4	42	theme	eosin	647:651	arg1	specimens					661:669	hematoxylin and eosin stained specimens	631:669	hematoxylin and eosin stained specimens using Image J software	631:692	The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software.
26492524	8	43	theme	hydroxyapatite/collagen	1208:1230	arg1	valid					1255:1259	valid	1255:1259	valid	1255:1259	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	8	43	theme	hydroxyapatite/collagen	1208:1230	arg1	material					1242:1249	The hydroxyapatite/collagen composite material	1204:1249	The hydroxyapatite/collagen composite material	1204:1249	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	1	44	theme	onlay	200:204	arg1	bone					206:209	newly formed onlay bone	187:209	newly formed onlay bone	187:209	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	0	45	theme	mouse	27:31	arg1	bone					43:46	mouse calvarial bone	27:46	mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma	27:138	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
26492524	1	46	theme	hydroxyapatite/collagen	275:297	arg1	composite					299:307	a hydroxyapatite/collagen composite	273:307	a hydroxyapatite/collagen composite	273:307	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	1	46	theme	hydroxyapatite/collagen	275:297	arg1	material					263:270	a new artificial bone material	241:270	a new artificial bone material	241:270	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	5	47	theme	greater	816:822	arg1	amount					824:829	a significantly greater amount	800:829	a significantly greater amount of newly formed bone than that with normal saline	800:879	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
26492524	5	47	theme	greater	816:822	arg1	bone					847:850	newly formed bone	834:850	newly formed bone than that with normal saline	834:879	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
26492524	8	48	theme	platelet-rich	1342:1354	arg1	plasma					1356:1361	platelet-rich plasma	1342:1361	platelet-rich plasma	1342:1361	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	5	49	theme	bone	847:850	arg1	amount					824:829	a significantly greater amount	800:829	a significantly greater amount of newly formed bone than that with normal saline	800:879	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
26492524	5	49	theme	bone	847:850	arg1	bone					847:850	newly formed bone	834:850	newly formed bone than that with normal saline	834:879	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
26492524	2	50	theme	normal	411:416	arg1	saline					418:423	normal saline	411:423	normal saline	411:423	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone.
26492524	8	51	theme	onlay	1265:1269	arg1	augmentation					1276:1287	onlay bone augmentation	1265:1287	onlay bone augmentation	1265:1287	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	8	52	theme	total	1327:1331	arg1	blood					1333:1337	total blood	1327:1337	total blood	1327:1337	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	2	53	with	material	397:404	arg1	saline					418:423	normal saline	411:423	normal saline	411:423	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone.
26492524	2	53	with	material	397:404	arg1	blood					432:436	total blood	426:436	total blood	426:436	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone.
26492524	2	53	with	material	397:404	arg1	plasma					455:460	platelet-rich plasma	441:460	platelet-rich plasma	441:460	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone.
26492524	6	54	theme	transplanted	949:960	arg1	materials					962:970	the transplanted materials	945:970	the transplanted materials with total blood or platelet-rich plasma but not with normal saline	945:1038	Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline.
26492524	7	55	theme	formed	1111:1116	arg1	bone					1118:1121	newly formed bone	1105:1121	newly formed bone	1105:1121	However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma.
26492524	7	56	theme	platelet-rich	1170:1182	arg1	plasma					1184:1189	platelet-rich plasma	1170:1189	platelet-rich plasma	1170:1189	However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma.
26492524	2	57	dep	DESIGN	352:357	arg1	material					397:404	The hydroxyapatite/collagen composite material	359:404	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma	352:460	DESIGN The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone.
26492524	4	58	theme	J	683:683	arg1	software					685:692	Image J software	677:692	Image J software	677:692	The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software.
26492524	1	59	theme	mouse	214:218	arg1	bone					230:233	mouse calvarial bone	214:233	mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite	214:307	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	0	60	theme	hydroxyapatite/collagen	56:78	arg1	material					90:97	a hydroxyapatite/collagen composite material	54:97	a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma	54:138	Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
26492524	7	61	from	differences	1076:1086	arg1	amount					1095:1100	the amount	1091:1100	the amount of newly formed bone	1091:1121	However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma.
26492524	7	61	from	differences	1076:1086	arg1	bone					1118:1121	newly formed bone	1105:1121	newly formed bone	1105:1121	However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma.
26492524	4	62	theme	hematoxylin	631:641	arg1	specimens					661:669	hematoxylin and eosin stained specimens	631:669	hematoxylin and eosin stained specimens using Image J software	631:692	The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software.
26492524	8	63	theme	bone	1271:1274	arg1	augmentation					1276:1287	onlay bone augmentation	1265:1287	onlay bone augmentation	1265:1287	CONCLUSIONS The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
26492524	1	64	theme	calvarial	220:228	arg1	bone					230:233	mouse calvarial bone	214:233	mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite	214:307	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	4	65	theme	formed	598:603	arg1	area					610:613	The newly formed bone area	588:613	The newly formed bone area	588:613	The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software.
26492524	1	66	theme	total	315:319	arg1	blood					321:325	total blood	315:325	total blood	315:325	OBJECTIVE The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
26492524	6	67	theme	normal	1026:1031	arg1	saline					1033:1038	normal saline	1026:1038	normal saline	1026:1038	Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline.
26492524	3	68	dep	weeks	567:571	arg1	surgery					579:585	surgery	579:585	surgery	579:585	The mice were sacrificed and the specimens were harvested four weeks after surgery.
26492524	5	69	dep	RESULTS	695:701	arg1	materials					741:749	The hydroxyapatite/collagen composite materials	703:749	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma	695:790	RESULTS The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline.
24983464	6	0	theme	fluoro-gold	926:936	arg1	tracing					949:955	fluoro-gold retrograde tracing	926:955	fluoro-gold retrograde tracing	926:955	16 weeks after implantation, electrophysiology, fluoro-gold retrograde tracing, and nerve morphometry were performed.
24983464	1	1	theme	peripheral	250:259	arg1	defects					267:273	peripheral nerve defects	250:273	peripheral nerve defects	250:273	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	9	2	theme	In	1227:1228	arg1	study					1242:1246	In vivo animal study	1227:1246	In vivo animal study	1227:1246	In vivo animal study demonstrated that the outcomes of NGF-CMSs/CCH were better than those of NGF/CCH or CCH.
24983464	3	3	theme	oriented	559:566	arg1	NGF-CMSs/CCH					583:594	NGF-CMSs/CCH	583:594	NGF-CMSs/CCH	583:594	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	3	3	theme	oriented	559:566	arg1	microchannels					568:580	longitudinally oriented microchannels	544:580	longitudinally oriented microchannels (NGF-CMSs/CCH)	544:595	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	1	4	theme	nerve	261:265	arg1	defects					267:273	peripheral nerve defects	250:273	peripheral nerve defects	250:273	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	0	5	theme	growth	108:113	arg1	factor					115:120	nerve growth factor	102:120	nerve growth factor	102:120	Incorporation of chitosan microspheres into collagen-chitosan scaffolds for the controlled release of nerve growth factor.
24983464	6	6	theme	retrograde	938:947	arg1	tracing					949:955	fluoro-gold retrograde tracing	926:955	fluoro-gold retrograde tracing	926:955	16 weeks after implantation, electrophysiology, fluoro-gold retrograde tracing, and nerve morphometry were performed.
24983464	10	7	theme	defects	1471:1477	arg1	repair					1444:1449	the repair	1440:1449	the repair of peripheral nerve defects	1440:1477	CONCLUSION Our findings suggest that incorporation of NGF-CMSs into the CCH may be a promising tool in the repair of peripheral nerve defects.
24983464	9	8	theme	animal	1235:1240	arg1	study					1242:1246	In vivo animal study	1227:1246	In vivo animal study	1227:1246	In vivo animal study demonstrated that the outcomes of NGF-CMSs/CCH were better than those of NGF/CCH or CCH.
24983464	2	9	theme	nerve	293:297	arg1	scaffolds					299:307	current nerve scaffolds	285:307	current nerve scaffolds	285:307	However, current nerve scaffolds lack efficient microstructure and neurotrophic support.
24983464	1	10	theme	defects	267:273	arg1	repair					240:245	the repair	236:245	the repair of peripheral nerve defects	236:273	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	0	11	theme	nerve	102:106	arg1	factor					115:120	nerve growth factor	102:120	nerve growth factor	102:120	Incorporation of chitosan microspheres into collagen-chitosan scaffolds for the controlled release of nerve growth factor.
24983464	4	12	dep	in	635:636	arg1	vitro					638:642	vitro	638:642	vitro	638:642	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	1	13	used	used	166:169	arg2	scaffold					150:157	BACKGROUND Artifical nerve scaffold	123:157	BACKGROUND Artifical nerve scaffold	123:157	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	1	13	used	used	166:169	arg2	alternative					186:196	a promising alternative	174:196	a promising alternative to autologous nerve grafts	174:223	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	8	14	theme	NGF	1174:1176	arg1	release					1153:1159	sustained release	1143:1159	sustained release of bioactive NGF within 28 days	1143:1191	The NGF-CMSs/CCH was capable of sustained release of bioactive NGF within 28 days as compared with others in vitro.
24983464	2	15	theme	current	285:291	arg1	scaffolds					299:307	current nerve scaffolds	285:307	current nerve scaffolds	285:307	However, current nerve scaffolds lack efficient microstructure and neurotrophic support.
24983464	4	16	theme	kinetics	652:659	arg1	characterizations					616:632	The morphological characterizations	598:632	The morphological characterizations	598:632	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	4	16	theme	kinetics	652:659	arg1	study					661:665	in vitro release kinetics study	635:665	in vitro release kinetics study	635:665	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	5	17	theme	nerve	861:865	arg1	autograft					867:875	nerve autograft	861:875	nerve autograft	861:875	After that, a 15-mm-long sciatic nerve gap in rats was bridged by the NGF-CMSs/CCH, CCH physically absorbed NGF (NGF/CCH), CCH or nerve autograft.
24983464	0	18	theme	factor	115:120	arg1	release					91:97	the controlled release	76:97	the controlled release of nerve growth factor	76:120	Incorporation of chitosan microspheres into collagen-chitosan scaffolds for the controlled release of nerve growth factor.
24983464	4	19	theme	release	644:650	arg1	characterizations					616:632	The morphological characterizations	598:632	The morphological characterizations	598:632	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	4	19	theme	release	644:650	arg1	study					661:665	in vitro release kinetics study	635:665	in vitro release kinetics study	635:665	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	10	20	theme	peripheral	1454:1463	arg1	defects					1471:1477	peripheral nerve defects	1454:1477	peripheral nerve defects	1454:1477	CONCLUSION Our findings suggest that incorporation of NGF-CMSs into the CCH may be a promising tool in the repair of peripheral nerve defects.
24983464	1	21	theme	promising	176:184	arg1	alternative					186:196	a promising alternative	174:196	a promising alternative to autologous nerve grafts	174:223	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	1	21	theme	promising	176:184	arg1	scaffold					150:157	BACKGROUND Artifical nerve scaffold	123:157	BACKGROUND Artifical nerve scaffold	123:157	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	8	22	theme	bioactive	1164:1172	arg1	NGF					1174:1176	bioactive NGF	1164:1176	bioactive NGF	1164:1176	The NGF-CMSs/CCH was capable of sustained release of bioactive NGF within 28 days as compared with others in vitro.
24983464	3	23	theme	growth	475:480	arg1	NGF-CMSs					490:497	NGF-CMSs	490:497	NGF-CMSs	490:497	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	3	23	theme	growth	475:480	arg1	factor					482:487	nerve growth factor	469:487	nerve growth factor (NGF-CMSs)	469:498	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	3	24	theme	chitosan	435:442	arg1	microspheres					444:455	chitosan microspheres	435:455	chitosan microspheres loaded with nerve growth factor (NGF-CMSs)	435:498	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	2	25	theme	efficient	314:322	arg1	microstructure					324:337	efficient microstructure	314:337	efficient microstructure	314:337	However, current nerve scaffolds lack efficient microstructure and neurotrophic support.
24983464	0	26	theme	microspheres	26:37	arg1	Incorporation					0:12	Incorporation	0:12	Incorporation of chitosan microspheres into collagen-chitosan	0:60	Incorporation of chitosan microspheres into collagen-chitosan scaffolds for the controlled release of nerve growth factor.
24983464	4	27	theme	morphological	602:614	arg1	study					661:665	in vitro release kinetics study	635:665	in vitro release kinetics study	635:665	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	4	27	theme	morphological	602:614	arg1	characterizations					616:632	The morphological characterizations	598:632	The morphological characterizations	598:632	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	4	27	theme	morphological	602:614	arg1	assay					709:713	bioactivity assay	697:713	bioactivity assay	697:713	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	4	27	theme	morphological	602:614	arg1	assay					686:690	neurite outgrowth assay	668:690	neurite outgrowth assay	668:690	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	5	28	theme	sciatic	756:762	arg1	gap					770:772	a 15-mm-long sciatic nerve gap	743:772	a 15-mm-long sciatic nerve gap in rats	743:780	After that, a 15-mm-long sciatic nerve gap in rats was bridged by the NGF-CMSs/CCH, CCH physically absorbed NGF (NGF/CCH), CCH or nerve autograft.
24983464	1	29	theme	autologous	201:210	arg1	grafts					218:223	autologous nerve grafts	201:223	autologous nerve grafts	201:223	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	0	30	theme	chitosan	17:24	arg1	microspheres					26:37	chitosan microspheres	17:37	chitosan microspheres	17:37	Incorporation of chitosan microspheres into collagen-chitosan scaffolds for the controlled release of nerve growth factor.
24983464	4	31	theme	in	635:636	arg1	characterizations					616:632	The morphological characterizations	598:632	The morphological characterizations	598:632	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	4	31	theme	in	635:636	arg1	study					661:665	in vitro release kinetics study	635:665	in vitro release kinetics study	635:665	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	5	32	theme	nerve	764:768	arg1	gap					770:772	a 15-mm-long sciatic nerve gap	743:772	a 15-mm-long sciatic nerve gap in rats	743:780	After that, a 15-mm-long sciatic nerve gap in rats was bridged by the NGF-CMSs/CCH, CCH physically absorbed NGF (NGF/CCH), CCH or nerve autograft.
24983464	1	33	theme	nerve	212:216	arg1	grafts					218:223	autologous nerve grafts	201:223	autologous nerve grafts	201:223	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	9	34	theme	NGF-CMSs/CCH	1282:1293	arg1	better					1300:1305	better	1300:1305	better	1300:1305	In vivo animal study demonstrated that the outcomes of NGF-CMSs/CCH were better than those of NGF/CCH or CCH.
24983464	9	34	theme	NGF-CMSs/CCH	1282:1293	arg1	outcomes					1270:1277	the outcomes	1266:1277	the outcomes of NGF-CMSs/CCH	1266:1293	In vivo animal study demonstrated that the outcomes of NGF-CMSs/CCH were better than those of NGF/CCH or CCH.
24983464	3	35	theme	nerve	469:473	arg1	NGF-CMSs					490:497	NGF-CMSs	490:497	NGF-CMSs	490:497	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	3	35	theme	nerve	469:473	arg1	factor					482:487	nerve growth factor	469:487	nerve growth factor (NGF-CMSs)	469:498	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	3	36	with	scaffolds	523:531	arg1	NGF-CMSs/CCH					583:594	NGF-CMSs/CCH	583:594	NGF-CMSs/CCH	583:594	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	3	36	with	scaffolds	523:531	arg1	microchannels					568:580	longitudinally oriented microchannels	544:580	longitudinally oriented microchannels (NGF-CMSs/CCH)	544:595	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	5	37	from	gap	770:772	arg1	rats					777:780	rats	777:780	rats	777:780	After that, a 15-mm-long sciatic nerve gap in rats was bridged by the NGF-CMSs/CCH, CCH physically absorbed NGF (NGF/CCH), CCH or nerve autograft.
24983464	10	38	theme	nerve	1465:1469	arg1	defects					1471:1477	peripheral nerve defects	1454:1477	peripheral nerve defects	1454:1477	CONCLUSION Our findings suggest that incorporation of NGF-CMSs into the CCH may be a promising tool in the repair of peripheral nerve defects.
24983464	3	39	theme	composite	394:402	arg1	METHODS					365:371	METHODS	365:371	METHODS Microsphere-Scaffold composite	365:402	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	7	40	theme	scaffold	1101:1108	arg1	microchannels					1080:1092	the longitudinally oriented microchannels	1052:1092	the longitudinally oriented microchannels of the scaffold	1052:1108	RESULTS The NGF-CMSs were evenly distributed throughout the longitudinally oriented microchannels of the scaffold.
24983464	10	41	theme	NGF-CMSs	1391:1398	arg1	tool					1432:1435	a promising tool	1420:1435	a promising tool	1420:1435	CONCLUSION Our findings suggest that incorporation of NGF-CMSs into the CCH may be a promising tool in the repair of peripheral nerve defects.
24983464	10	41	theme	NGF-CMSs	1391:1398	arg1	incorporation					1374:1386	incorporation	1374:1386	incorporation of NGF-CMSs into the CCH	1374:1411	CONCLUSION Our findings suggest that incorporation of NGF-CMSs into the CCH may be a promising tool in the repair of peripheral nerve defects.
24983464	1	42	theme	BACKGROUND	123:132	arg1	alternative					186:196	a promising alternative	174:196	a promising alternative to autologous nerve grafts	174:223	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	1	42	theme	BACKGROUND	123:132	arg1	scaffold					150:157	BACKGROUND Artifical nerve scaffold	123:157	BACKGROUND Artifical nerve scaffold	123:157	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	5	43	dep	NGF-CMSs/CCH	801:812	arg1	absorbed					830:837	absorbed	830:837	absorbed	830:837	After that, a 15-mm-long sciatic nerve gap in rats was bridged by the NGF-CMSs/CCH, CCH physically absorbed NGF (NGF/CCH), CCH or nerve autograft.
24983464	8	44	theme	sustained	1143:1151	arg1	release					1153:1159	sustained release	1143:1159	sustained release of bioactive NGF within 28 days	1143:1191	The NGF-CMSs/CCH was capable of sustained release of bioactive NGF within 28 days as compared with others in vitro.
24983464	1	45	theme	Artifical	134:142	arg1	alternative					186:196	a promising alternative	174:196	a promising alternative to autologous nerve grafts	174:223	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	1	45	theme	Artifical	134:142	arg1	scaffold					150:157	BACKGROUND Artifical nerve scaffold	123:157	BACKGROUND Artifical nerve scaffold	123:157	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	6	46	theme	nerve	962:966	arg1	morphometry					968:978	nerve morphometry	962:978	nerve morphometry	962:978	16 weeks after implantation, electrophysiology, fluoro-gold retrograde tracing, and nerve morphometry were performed.
24983464	1	47	theme	nerve	144:148	arg1	alternative					186:196	a promising alternative	174:196	a promising alternative to autologous nerve grafts	174:223	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	1	47	theme	nerve	144:148	arg1	scaffold					150:157	BACKGROUND Artifical nerve scaffold	123:157	BACKGROUND Artifical nerve scaffold	123:157	BACKGROUND Artifical nerve scaffold can be used as a promising alternative to autologous nerve grafts to enhance the repair of peripheral nerve defects.
24983464	7	48	dep	RESULTS	996:1002	arg1	distributed					1029:1039	distributed	1029:1039	were evenly distributed throughout the longitudinally oriented microchannels of the scaffold	1017:1108	RESULTS The NGF-CMSs were evenly distributed throughout the longitudinally oriented microchannels of the scaffold.
24983464	4	49	theme	neurite	668:674	arg1	characterizations					616:632	The morphological characterizations	598:632	The morphological characterizations	598:632	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	4	49	theme	neurite	668:674	arg1	assay					686:690	neurite outgrowth assay	668:690	neurite outgrowth assay	668:690	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	5	50	theme	15-mm-long	745:754	arg1	gap					770:772	a 15-mm-long sciatic nerve gap	743:772	a 15-mm-long sciatic nerve gap in rats	743:780	After that, a 15-mm-long sciatic nerve gap in rats was bridged by the NGF-CMSs/CCH, CCH physically absorbed NGF (NGF/CCH), CCH or nerve autograft.
24983464	7	51	theme	oriented	1071:1078	arg1	microchannels					1080:1092	the longitudinally oriented microchannels	1052:1092	the longitudinally oriented microchannels of the scaffold	1052:1108	RESULTS The NGF-CMSs were evenly distributed throughout the longitudinally oriented microchannels of the scaffold.
24983464	10	52	from	tool	1432:1435	arg1	repair					1444:1449	the repair	1440:1449	the repair of peripheral nerve defects	1440:1477	CONCLUSION Our findings suggest that incorporation of NGF-CMSs into the CCH may be a promising tool in the repair of peripheral nerve defects.
24983464	9	53	dep	In	1227:1228	arg1	vivo					1230:1233	vivo	1230:1233	vivo	1230:1233	In vivo animal study demonstrated that the outcomes of NGF-CMSs/CCH were better than those of NGF/CCH or CCH.
24983464	2	54	theme	neurotrophic	343:354	arg1	support					356:362	neurotrophic support	343:362	neurotrophic support	343:362	However, current nerve scaffolds lack efficient microstructure and neurotrophic support.
24983464	0	55	theme	controlled	80:89	arg1	release					91:97	the controlled release	76:97	the controlled release of nerve growth factor	76:120	Incorporation of chitosan microspheres into collagen-chitosan scaffolds for the controlled release of nerve growth factor.
24983464	3	56	theme	collagen-chitosan	505:521	arg1	CCH					534:536	CCH	534:536	CCH	534:536	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	3	56	theme	collagen-chitosan	505:521	arg1	scaffolds					523:531	collagen-chitosan scaffolds	505:531	collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH)	505:595	METHODS Microsphere-Scaffold composite was developed by incorporating chitosan microspheres loaded with nerve growth factor (NGF-CMSs) into collagen-chitosan scaffolds (CCH) with longitudinally oriented microchannels (NGF-CMSs/CCH).
24983464	10	57	theme	promising	1422:1430	arg1	tool					1432:1435	a promising tool	1420:1435	a promising tool	1420:1435	CONCLUSION Our findings suggest that incorporation of NGF-CMSs into the CCH may be a promising tool in the repair of peripheral nerve defects.
24983464	10	57	theme	promising	1422:1430	arg1	incorporation					1374:1386	incorporation	1374:1386	incorporation of NGF-CMSs into the CCH	1374:1411	CONCLUSION Our findings suggest that incorporation of NGF-CMSs into the CCH may be a promising tool in the repair of peripheral nerve defects.
24983464	5	58	theme	NGF-CMSs/CCH	801:812	arg1	NGF					839:841	the NGF-CMSs/CCH, CCH physically absorbed NGF	797:841	the NGF-CMSs/CCH, CCH physically absorbed NGF (NGF/CCH)	797:851	After that, a 15-mm-long sciatic nerve gap in rats was bridged by the NGF-CMSs/CCH, CCH physically absorbed NGF (NGF/CCH), CCH or nerve autograft.
24983464	5	58	theme	NGF-CMSs/CCH	801:812	arg1	NGF/CCH					844:850	NGF/CCH	844:850	NGF/CCH	844:850	After that, a 15-mm-long sciatic nerve gap in rats was bridged by the NGF-CMSs/CCH, CCH physically absorbed NGF (NGF/CCH), CCH or nerve autograft.
24983464	10	59	dep	CONCLUSION	1337:1346	arg1	suggest					1361:1367	suggest	1361:1367	suggest that incorporation of NGF-CMSs into the CCH may be a promising tool in the repair of peripheral nerve defects	1361:1477	CONCLUSION Our findings suggest that incorporation of NGF-CMSs into the CCH may be a promising tool in the repair of peripheral nerve defects.
24983464	8	60	theme	release	1153:1159	arg1	NGF-CMSs/CCH					1115:1126	The NGF-CMSs/CCH	1111:1126	The NGF-CMSs/CCH	1111:1126	The NGF-CMSs/CCH was capable of sustained release of bioactive NGF within 28 days as compared with others in vitro.
24983464	8	60	theme	release	1153:1159	arg1	capable					1132:1138	capable	1132:1138	capable	1132:1138	The NGF-CMSs/CCH was capable of sustained release of bioactive NGF within 28 days as compared with others in vitro.
24983464	4	61	theme	outgrowth	676:684	arg1	characterizations					616:632	The morphological characterizations	598:632	The morphological characterizations	598:632	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24983464	4	61	theme	outgrowth	676:684	arg1	assay					686:690	neurite outgrowth assay	668:690	neurite outgrowth assay	668:690	The morphological characterizations, in vitro release kinetics study, neurite outgrowth assay, and bioactivity assay were evaluated.
24495557	0	0	theme	fibroin/hyaluronan	80:97	arg1	scaffolds					99:107	silk fibroin/hyaluronan scaffolds	75:107	silk fibroin/hyaluronan scaffolds	75:107	Effect of hyaluronan molecular weight on structure and biocompatibility of silk fibroin/hyaluronan scaffolds.
24495557	2	1	theme	composite	512:520	arg1	scaffolds					522:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	4	2	theme	75	795:796	arg1	%					797:797	%	797:797	%	797:797	The 3D pore structure maintained the scaffold shape during the process of 75% ethanol annealing.
24495557	6	3	theme	SF/HA	977:981	arg1	scaffolds					983:991	SF/HA scaffolds	977:991	SF/HA scaffolds	977:991	In addition, SF/HA scaffolds showed an increase in cell proliferation compared to pure SF scaffold.
24495557	0	4	theme	silk	75:78	arg1	scaffolds					99:107	silk fibroin/hyaluronan scaffolds	75:107	silk fibroin/hyaluronan scaffolds	75:107	Effect of hyaluronan molecular weight on structure and biocompatibility of silk fibroin/hyaluronan scaffolds.
24495557	2	5	theme	/HA	508:510	arg1	scaffolds					522:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	1	6	theme	key	156:158	arg1	role					160:163	a key role	154:163	a key role	154:163	The structure of scaffolds is known to play a key role in tissue engineering as it provides structural support and physical environment allowing cells to reside and rebuild the target tissue.
24495557	0	7	from	Effect	0:5	arg1	biocompatibility					55:70	biocompatibility	55:70	biocompatibility	55:70	Effect of hyaluronan molecular weight on structure and biocompatibility of silk fibroin/hyaluronan scaffolds.
24495557	0	7	from	Effect	0:5	arg1	structure					41:49	structure	41:49	structure	41:49	Effect of hyaluronan molecular weight on structure and biocompatibility of silk fibroin/hyaluronan scaffolds.
24495557	6	8	theme	SF	1051:1052	arg1	scaffold					1054:1061	pure SF scaffold	1046:1061	pure SF scaffold	1046:1061	In addition, SF/HA scaffolds showed an increase in cell proliferation compared to pure SF scaffold.
24495557	0	9	theme	scaffolds	99:107	arg1	biocompatibility					55:70	biocompatibility	55:70	biocompatibility	55:70	Effect of hyaluronan molecular weight on structure and biocompatibility of silk fibroin/hyaluronan scaffolds.
24495557	0	9	theme	scaffolds	99:107	arg1	structure					41:49	structure	41:49	structure	41:49	Effect of hyaluronan molecular weight on structure and biocompatibility of silk fibroin/hyaluronan scaffolds.
24495557	3	10	theme	sheet	639:643	arg1	structures					650:659	separate sheet like structures	630:659	separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW	630:718	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	2	11	theme	SF	505:506	arg1	scaffolds					522:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	7	12	theme	suitable	1155:1162	arg1	scaffolds					1145:1153	SF/HA blend scaffolds	1133:1153	SF/HA blend scaffolds suitable for application in tissue engineering	1133:1200	These findings demonstrated the important role of HA MW in preparing SF/HA blend scaffolds suitable for application in tissue engineering.
24495557	3	13	theme	like	645:648	arg1	structures					650:659	separate sheet like structures	630:659	separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW	630:718	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	2	14	dep	1.6	389:391	arg1	6					404:404	6	404:404	6	404:404	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	4	15	theme	pore	728:731	arg1	structure					733:741	The 3D pore structure	721:741	The 3D pore structure	721:741	The 3D pore structure maintained the scaffold shape during the process of 75% ethanol annealing.
24495557	2	16	dep	hyaluronan	346:355	arg1	weight					372:377	molecular weight	362:377	hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da)	346:409	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	2	17	theme	molecular	362:370	arg1	weight					372:377	molecular weight	362:377	hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da)	346:409	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	3	18	theme	separate	630:637	arg1	structures					650:659	separate sheet like structures	630:659	separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW	630:718	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	6	19	dep	showed	993:998	arg1	compared					1034:1041	compared	1034:1041	showed an increase in cell proliferation compared to pure SF scaffold	993:1061	In addition, SF/HA scaffolds showed an increase in cell proliferation compared to pure SF scaffold.
24495557	2	20	from	effects	335:341	arg1	biocompatibility					459:474	biocompatibility	459:474	biocompatibility	459:474	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	2	20	from	effects	335:341	arg1	structure					423:431	the pore structure	414:431	the pore structure	414:431	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	2	20	from	effects	335:341	arg1	structure					444:452	secondary structure	434:452	secondary structure	434:452	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	6	21	theme	pure	1046:1049	arg1	scaffold					1054:1061	pure SF scaffold	1046:1061	pure SF scaffold	1046:1061	In addition, SF/HA scaffolds showed an increase in cell proliferation compared to pure SF scaffold.
24495557	1	22	theme	tissue	168:173	arg1	engineering					175:185	tissue engineering	168:185	tissue engineering	168:185	The structure of scaffolds is known to play a key role in tissue engineering as it provides structural support and physical environment allowing cells to reside and rebuild the target tissue.
24495557	2	23	theme	scaffolds	522:530	arg1	biocompatibility					459:474	biocompatibility	459:474	biocompatibility	459:474	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	2	23	theme	scaffolds	522:530	arg1	structure					423:431	the pore structure	414:431	the pore structure	414:431	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	2	23	theme	scaffolds	522:530	arg1	structure					444:452	secondary structure	434:452	secondary structure	434:452	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	3	24	from	structures	650:659	arg1	scaffolds					680:688	the SF/HA blend scaffolds	664:688	the SF/HA blend scaffolds	664:688	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	3	24	from	structures	650:659	arg1	dependent					701:709	dependent	701:709	dependent	701:709	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	5	25	from	coil	920:923	arg1	transition					897:906	SF conformation transition	881:906	SF conformation transition from random coil to β-sheet	881:934	Structural studies indicated that HA did not induce but hinder SF conformation transition from random coil to β-sheet before and after treatment.
24495557	3	26	from	scaffolds	680:688	arg1	formation					617:625	the formation	613:625	the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW	613:718	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	0	27	theme	molecular	21:29	arg1	weight					31:36	hyaluronan molecular weight	10:36	hyaluronan molecular weight	10:36	Effect of hyaluronan molecular weight on structure and biocompatibility of silk fibroin/hyaluronan scaffolds.
24495557	3	28	theme	SF/HA	668:672	arg1	scaffolds					680:688	the SF/HA blend scaffolds	664:688	the SF/HA blend scaffolds	664:688	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	3	28	theme	SF/HA	668:672	arg1	dependent					701:709	dependent	701:709	dependent	701:709	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	4	29	theme	annealing	807:815	arg1	process					784:790	the process	780:790	the process of 75% ethanol annealing	780:815	The 3D pore structure maintained the scaffold shape during the process of 75% ethanol annealing.
24495557	7	30	from	application	1168:1178	arg1	engineering					1190:1200	tissue engineering	1183:1200	tissue engineering	1183:1200	These findings demonstrated the important role of HA MW in preparing SF/HA blend scaffolds suitable for application in tissue engineering.
24495557	4	31	theme	scaffold	758:765	arg1	shape					767:771	the scaffold shape	754:771	the scaffold shape	754:771	The 3D pore structure maintained the scaffold shape during the process of 75% ethanol annealing.
24495557	0	32	theme	hyaluronan	10:19	arg1	weight					31:36	hyaluronan molecular weight	10:36	hyaluronan molecular weight	10:36	Effect of hyaluronan molecular weight on structure and biocompatibility of silk fibroin/hyaluronan scaffolds.
24495557	3	33	theme	structures	650:659	arg1	formation					617:625	the formation	613:625	the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW	613:718	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	4	34	theme	ethanol	799:805	arg1	annealing					807:815	75% ethanol annealing	795:815	75% ethanol annealing	795:815	The 3D pore structure maintained the scaffold shape during the process of 75% ethanol annealing.
24495557	7	35	theme	tissue	1183:1188	arg1	engineering					1190:1200	tissue engineering	1183:1200	tissue engineering	1183:1200	These findings demonstrated the important role of HA MW in preparing SF/HA blend scaffolds suitable for application in tissue engineering.
24495557	1	36	theme	target	287:292	arg1	tissue					294:299	the target tissue	283:299	the target tissue	283:299	The structure of scaffolds is known to play a key role in tissue engineering as it provides structural support and physical environment allowing cells to reside and rebuild the target tissue.
24495557	2	37	dep	MW	380:381	arg1	Da					407:408	0.6, 1.6 and 2.6×10(6) Da	384:408	MW: 0.6, 1.6 and 2.6×10(6) Da	380:408	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	0	38	theme	weight	31:36	arg1	Effect					0:5	Effect	0:5	Effect of hyaluronan molecular weight on structure and biocompatibility of silk fibroin/hyaluronan scaffolds.	0:108	Effect of hyaluronan molecular weight on structure and biocompatibility of silk fibroin/hyaluronan scaffolds.
24495557	5	39	theme	random	913:918	arg1	coil					920:923	random coil	913:923	random coil	913:923	Structural studies indicated that HA did not induce but hinder SF conformation transition from random coil to β-sheet before and after treatment.
24495557	6	40	from	increase	1003:1010	arg1	proliferation					1020:1032	cell proliferation	1015:1032	cell proliferation	1015:1032	In addition, SF/HA scaffolds showed an increase in cell proliferation compared to pure SF scaffold.
24495557	1	41	theme	structural	202:211	arg1	support					213:219	structural support	202:219	structural support	202:219	The structure of scaffolds is known to play a key role in tissue engineering as it provides structural support and physical environment allowing cells to reside and rebuild the target tissue.
24495557	7	42	theme	HA	1114:1115	arg1	MW					1117:1118	HA MW	1114:1118	HA MW	1114:1118	These findings demonstrated the important role of HA MW in preparing SF/HA blend scaffolds suitable for application in tissue engineering.
24495557	4	43	theme	3D	725:726	arg1	structure					733:741	The 3D pore structure	721:741	The 3D pore structure	721:741	The 3D pore structure maintained the scaffold shape during the process of 75% ethanol annealing.
24495557	4	44	theme	%	797:797	arg1	annealing					807:815	75% ethanol annealing	795:815	75% ethanol annealing	795:815	The 3D pore structure maintained the scaffold shape during the process of 75% ethanol annealing.
24495557	6	45	theme	cell	1015:1018	arg1	proliferation					1020:1032	cell proliferation	1015:1032	cell proliferation	1015:1032	In addition, SF/HA scaffolds showed an increase in cell proliferation compared to pure SF scaffold.
24495557	3	46	from	formation	617:625	arg1	scaffolds					680:688	the SF/HA blend scaffolds	664:688	the SF/HA blend scaffolds	664:688	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	3	46	from	formation	617:625	arg1	dependent					701:709	dependent	701:709	dependent	701:709	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	3	47	theme	blend	674:678	arg1	scaffolds					680:688	the SF/HA blend scaffolds	664:688	the SF/HA blend scaffolds	664:688	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	3	47	theme	blend	674:678	arg1	dependent					701:709	dependent	701:709	dependent	701:709	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	2	48	theme	fibroin	496:502	arg1	scaffolds					522:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	3	49	theme	structure	578:586	arg1	formation					588:596	the pore structure formation	569:596	the pore structure formation	569:596	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	7	50	theme	important	1096:1104	arg1	role					1106:1109	the important role	1092:1109	the important role of HA MW in preparing SF/HA blend scaffolds suitable for application in tissue engineering	1092:1200	These findings demonstrated the important role of HA MW in preparing SF/HA blend scaffolds suitable for application in tissue engineering.
24495557	1	51	theme	scaffolds	127:135	arg1	structure					114:122	The structure	110:122	The structure of scaffolds	110:135	The structure of scaffolds is known to play a key role in tissue engineering as it provides structural support and physical environment allowing cells to reside and rebuild the target tissue.
24495557	7	52	theme	blend	1139:1143	arg1	scaffolds					1145:1153	SF/HA blend scaffolds	1133:1153	SF/HA blend scaffolds suitable for application in tissue engineering	1133:1200	These findings demonstrated the important role of HA MW in preparing SF/HA blend scaffolds suitable for application in tissue engineering.
24495557	2	53	theme	silk	491:494	arg1	scaffolds					522:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	2	54	theme	lyophilized	479:489	arg1	scaffolds					522:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	lyophilized silk fibroin (SF)/HA composite scaffolds	479:530	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	5	55	theme	SF	881:882	arg1	transition					897:906	SF conformation transition	881:906	SF conformation transition from random coil to β-sheet	881:934	Structural studies indicated that HA did not induce but hinder SF conformation transition from random coil to β-sheet before and after treatment.
24495557	5	56	theme	Structural	818:827	arg1	studies					829:835	Structural studies	818:835	Structural studies	818:835	Structural studies indicated that HA did not induce but hinder SF conformation transition from random coil to β-sheet before and after treatment.
24495557	3	57	theme	HA	714:715	arg1	MW					717:718	HA MW	714:718	HA MW	714:718	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	2	58	theme	pore	418:421	arg1	structure					423:431	the pore structure	414:431	the pore structure	414:431	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	3	59	theme	pore	573:576	arg1	formation					588:596	the pore structure formation	569:596	the pore structure formation	569:596	The results showed that HA promoted the pore structure formation and restrained the formation of separate sheet like structures in the SF/HA blend scaffolds, which was dependent on HA MW.
24495557	2	60	dep	weight	372:377	arg1	MW					380:381	MW	380:381	MW: 0.6, 1.6 and 2.6×10(6) Da	380:408	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	7	61	theme	SF/HA	1133:1137	arg1	scaffolds					1145:1153	SF/HA blend scaffolds	1133:1153	SF/HA blend scaffolds suitable for application in tissue engineering	1133:1200	These findings demonstrated the important role of HA MW in preparing SF/HA blend scaffolds suitable for application in tissue engineering.
24495557	5	62	theme	conformation	884:895	arg1	transition					897:906	SF conformation transition	881:906	SF conformation transition from random coil to β-sheet	881:934	Structural studies indicated that HA did not induce but hinder SF conformation transition from random coil to β-sheet before and after treatment.
24495557	1	63	theme	physical	225:232	arg1	environment					234:244	physical environment	225:244	physical environment	225:244	The structure of scaffolds is known to play a key role in tissue engineering as it provides structural support and physical environment allowing cells to reside and rebuild the target tissue.
24495557	7	64	theme	MW	1117:1118	arg1	role					1106:1109	the important role	1092:1109	the important role of HA MW in preparing SF/HA blend scaffolds suitable for application in tissue engineering	1092:1200	These findings demonstrated the important role of HA MW in preparing SF/HA blend scaffolds suitable for application in tissue engineering.
24495557	2	65	theme	hyaluronan	346:355	arg1	effects					335:341	the effects	331:341	the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds	331:530	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
24495557	2	66	theme	secondary	434:442	arg1	structure					444:452	secondary structure	434:452	secondary structure	434:452	In this work we investigated the effects of hyaluronan (HA) molecular weight (MW: 0.6, 1.6 and 2.6×10(6) Da) on the pore structure, secondary structure, and biocompatibility of lyophilized silk fibroin (SF)/HA composite scaffolds.
27568483	0	0	theme	blast	73:77	arg1	wall					91:94	the rice blast fungal cell wall	64:94	the rice blast fungal cell wall	64:94	The β-1,3-glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium-mediated plant infection.
27568483	6	1	theme	null	1004:1007	arg1	mutant					1009:1014	a Δgel1Δgel3Δgel4 null mutant	986:1014	a Δgel1Δgel3Δgel4 null mutant	986:1014	Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain.
27568483	1	2	theme	host	235:238	arg1	infection					240:248	host infection	235:248	host infection	235:248	The fungal wall is pivotal for cell shape and function, and in interfacial protection during host infection and environmental challenge.
27568483	0	3	theme	rice	68:71	arg1	wall					91:94	the rice blast fungal cell wall	64:94	the rice blast fungal cell wall	64:94	The β-1,3-glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium-mediated plant infection.
27568483	8	4	theme	fungal	1444:1449	arg1	wall					1456:1459	the fungal cell wall	1440:1459	the fungal cell wall	1440:1459	We conclude that Gel proteins play significant roles in structural modification of the fungal cell wall during appressorium-mediated plant infection.
27568483	8	5	theme	structural	1413:1422	arg1	modification					1424:1435	structural modification	1413:1435	structural modification of the fungal cell wall	1413:1459	We conclude that Gel proteins play significant roles in structural modification of the fungal cell wall during appressorium-mediated plant infection.
27568483	2	6	theme	composition	338:348	arg1	description					306:316	the first description	296:316	the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae	296:423	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	4	7	theme	putative	702:709	arg1	module					732:737	a putative carbohydrate-binding module	700:737	a putative carbohydrate-binding module	700:737	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	0	8	theme	cell	86:89	arg1	wall					91:94	the rice blast fungal cell wall	64:94	the rice blast fungal cell wall	64:94	The β-1,3-glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium-mediated plant infection.
27568483	7	9	theme	expression	1219:1228	arg1	patterns					1202:1209	global patterns	1195:1209	global patterns of gene expression, a hyper-branching phenotype and no sporulation	1195:1276	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	7	10	theme	rice	1308:1311	arg1	lesions					1319:1325	rice blast lesions	1308:1325	rice blast lesions	1308:1325	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	3	11	theme	putative	508:515	arg1	glucanosyltransferases					530:551	five putative β-1,3-glucan glucanosyltransferases	503:551	five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature	503:604	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	4	12	theme	carbohydrate-binding	711:730	arg1	module					732:737	a putative carbohydrate-binding module	700:737	a putative carbohydrate-binding module	700:737	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	0	13	theme	fungal	79:84	arg1	wall					91:94	the rice blast fungal cell wall	64:94	the rice blast fungal cell wall	64:94	The β-1,3-glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium-mediated plant infection.
27568483	8	14	theme	wall	1456:1459	arg1	modification					1424:1435	structural modification	1413:1435	structural modification of the fungal cell wall	1413:1459	We conclude that Gel proteins play significant roles in structural modification of the fungal cell wall during appressorium-mediated plant infection.
27568483	3	15	theme	β-1,3-glucan	517:528	arg1	glucanosyltransferases					530:551	five putative β-1,3-glucan glucanosyltransferases	503:551	five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature	503:604	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	7	16	theme	global	1195:1200	arg1	patterns					1202:1209	global patterns	1195:1209	global patterns of gene expression, a hyper-branching phenotype and no sporulation	1195:1276	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	2	17	dep	composition	338:348	arg1	the					321:323	the	321:323	the	321:323	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	1	18	theme	environmental	254:266	arg1	challenge					268:276	environmental challenge	254:276	environmental challenge	254:276	The fungal wall is pivotal for cell shape and function, and in interfacial protection during host infection and environmental challenge.
27568483	3	19	theme	elongation	459:468	arg1	Gels					480:483	Gels	480:483	Gels	480:483	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	3	19	theme	elongation	459:468	arg1	proteins					470:477	glucan elongation proteins	452:477	glucan elongation proteins (Gels)	452:484	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	5	20	theme	M.	795:796	arg1	GELs					811:814	M. oryzae GH72+ GELs	795:814	M. oryzae GH72+ GELs	795:814	We reveal that M. oryzae GH72+ GELs are expressed in spores and during both infective and vegetative growth, but each individual Gel enzymes are dispensable for pathogenicity.
27568483	4	21	theme	targeted	620:627	arg1	mutants					638:644	targeted deletion mutants	620:644	targeted deletion mutants	620:644	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	4	21	theme	targeted	620:627	arg1	GH72+					680:684	the GH72+	676:684	the GH72+	676:684	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	4	22	theme	GH72-	748:752	arg1	Gels					754:757	the GH72- Gels	744:757	the GH72- Gels	744:757	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	7	23	theme	sporulation	1266:1276	arg1	patterns					1202:1209	global patterns	1195:1209	global patterns of gene expression, a hyper-branching phenotype and no sporulation	1195:1276	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	4	24	theme	Gel	653:655	arg1	isoforms					657:664	all Gel isoforms	649:664	all Gel isoforms	649:664	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	0	25	theme	wall	91:94	arg1	structure					51:59	the structure	47:59	the structure of the rice blast fungal cell wall	47:94	The β-1,3-glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium-mediated plant infection.
27568483	8	26	theme	appressorium-mediated	1468:1488	arg1	infection					1496:1504	appressorium-mediated plant infection	1468:1504	appressorium-mediated plant infection	1468:1504	We conclude that Gel proteins play significant roles in structural modification of the fungal cell wall during appressorium-mediated plant infection.
27568483	7	27	theme	no	1263:1264	arg1	sporulation					1266:1276	no sporulation	1263:1276	no sporulation	1263:1276	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	2	28	theme	wall	376:379	arg1	structure					354:362	structure	354:362	structure	354:362	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	2	28	theme	wall	376:379	arg1	composition					338:348	carbohydrate composition	325:348	carbohydrate composition	325:348	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	7	29	theme	hyper-branching	1233:1247	arg1	phenotype					1249:1257	a hyper-branching phenotype	1231:1257	a hyper-branching phenotype	1231:1257	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	2	30	theme	first	300:304	arg1	description					306:316	the first description	296:316	the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae	296:423	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	4	31	contain	carry	694:698	arg1	mutants					638:644	targeted deletion mutants	620:644	targeted deletion mutants	620:644	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	4	31	contain	carry	694:698	arg2	Gels					754:757	the GH72- Gels	744:757	the GH72- Gels	744:757	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	4	31	contain	carry	694:698	arg2	module					732:737	a putative carbohydrate-binding module	700:737	a putative carbohydrate-binding module	700:737	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	4	31	contain	carry	694:698	arg1	GH72+					680:684	the GH72+	676:684	the GH72+	676:684	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	2	32	theme	cell	371:374	arg1	wall					376:379	the cell wall	367:379	the cell wall of the rice blast fungus Magnaporthe oryzae	367:423	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	7	33	from	differences	1180:1190	arg1	patterns					1202:1209	global patterns	1195:1209	global patterns of gene expression, a hyper-branching phenotype and no sporulation	1195:1276	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	4	34	theme	isoforms	657:664	arg1	mutants					638:644	targeted deletion mutants	620:644	targeted deletion mutants	620:644	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	4	34	theme	isoforms	657:664	arg1	GH72+					680:684	the GH72+	676:684	the GH72+	676:684	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	6	35	theme	modified	1022:1029	arg1	wall					1036:1039	a modified cell wall	1020:1039	a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain	1020:1147	Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain.
27568483	0	36	theme	plant	125:129	arg1	infection					131:139	appressorium-mediated plant infection	103:139	appressorium-mediated plant infection	103:139	The β-1,3-glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium-mediated plant infection.
27568483	7	37	theme	blast	1313:1317	arg1	lesions					1319:1325	rice blast lesions	1308:1325	rice blast lesions	1308:1325	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	5	38	theme	each	893:896	arg1	enzymes					913:919	each individual Gel enzymes	893:919	each individual Gel enzymes	893:919	We reveal that M. oryzae GH72+ GELs are expressed in spores and during both infective and vegetative growth, but each individual Gel enzymes are dispensable for pathogenicity.
27568483	3	39	theme	Hydrolase	583:591	arg1	signature					596:604	the Glycoside Hydrolase 72 signature	569:604	the Glycoside Hydrolase 72 signature	569:604	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	2	40	theme	fungus	399:404	arg1	wall					376:379	the cell wall	367:379	the cell wall of the rice blast fungus Magnaporthe oryzae	367:423	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	2	41	theme	Magnaporthe	406:416	arg1	oryzae					418:423	Magnaporthe oryzae	406:423	the rice blast fungus Magnaporthe oryzae	384:423	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	3	42	contain	carry	563:567	arg2	signature					596:604	the Glycoside Hydrolase 72 signature	569:604	the Glycoside Hydrolase 72 signature	569:604	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	3	42	contain	carry	563:567	arg1	glucanosyltransferases					530:551	five putative β-1,3-glucan glucanosyltransferases	503:551	five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature	503:604	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	0	43	theme	appressorium-mediated	103:123	arg1	infection					131:139	appressorium-mediated plant infection	103:139	appressorium-mediated plant infection	103:139	The β-1,3-glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium-mediated plant infection.
27568483	6	44	contain	have	1062:1065	arg2	degree					1076:1081	a higher degree	1067:1081	a higher degree of polymerization	1067:1099	Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain.
27568483	6	44	contain	have	1062:1065	arg1	1,3-glucans					1050:1060	1,3-glucans	1050:1060	1,3-glucans	1050:1060	Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain.
27568483	3	45	dep	carry	563:567	arg1	each					558:561	each	558:561	each	558:561	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	2	46	theme	blast	393:397	arg1	fungus					399:404	the rice blast fungus Magnaporthe oryzae	384:423	the rice blast fungus Magnaporthe oryzae	384:423	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	6	47	theme	higher	1069:1074	arg1	degree					1076:1081	a higher degree	1067:1081	a higher degree of polymerization	1067:1099	Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain.
27568483	6	48	theme	Δgel1Δgel3Δgel4	988:1002	arg1	mutant					1009:1014	a Δgel1Δgel3Δgel4 null mutant	986:1014	a Δgel1Δgel3Δgel4 null mutant	986:1014	Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain.
27568483	1	49	dep	cell	173:176	arg1	shape					178:182	shape	178:182	shape	178:182	The fungal wall is pivotal for cell shape and function, and in interfacial protection during host infection and environmental challenge.
27568483	2	50	theme	rice	388:391	arg1	fungus					399:404	the rice blast fungus Magnaporthe oryzae	384:423	the rice blast fungus Magnaporthe oryzae	384:423	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	6	51	theme	cell	1031:1034	arg1	wall					1036:1039	a modified cell wall	1020:1039	a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain	1020:1147	Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain.
27568483	5	52	theme	GH72+	805:809	arg1	GELs					811:814	M. oryzae GH72+ GELs	795:814	M. oryzae GH72+ GELs	795:814	We reveal that M. oryzae GH72+ GELs are expressed in spores and during both infective and vegetative growth, but each individual Gel enzymes are dispensable for pathogenicity.
27568483	1	53	from	protection	217:226	arg1	pivotal					161:167	pivotal	161:167	pivotal	161:167	The fungal wall is pivotal for cell shape and function, and in interfacial protection during host infection and environmental challenge.
27568483	8	54	theme	plant	1490:1494	arg1	infection					1496:1504	appressorium-mediated plant infection	1468:1504	appressorium-mediated plant infection	1468:1504	We conclude that Gel proteins play significant roles in structural modification of the fungal cell wall during appressorium-mediated plant infection.
27568483	2	55	dep	fungus	399:404	arg1	oryzae					418:423	Magnaporthe oryzae	406:423	the rice blast fungus Magnaporthe oryzae	384:423	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	2	56	theme	carbohydrate	325:336	arg1	composition					338:348	carbohydrate composition	325:348	carbohydrate composition	325:348	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
27568483	7	57	theme	wounded	1339:1345	arg1	tissues					1347:1353	wounded tissues	1339:1353	wounded tissues	1339:1353	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	7	58	theme	gene	1214:1217	arg1	expression					1219:1228	gene expression	1214:1228	gene expression	1214:1228	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	3	59	theme	Glycoside	573:581	arg1	Hydrolase					583:591	Glycoside Hydrolase 72	573:594	the Glycoside Hydrolase 72 signature	569:604	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	3	60	theme	proteins	470:477	arg1	family					442:447	the family	438:447	the family of glucan elongation proteins (Gels)	438:484	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	6	61	theme	wild-type	1132:1140	arg1	strain					1142:1147	the wild-type strain	1128:1147	the wild-type strain	1128:1147	Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain.
27568483	8	62	theme	significant	1392:1402	arg1	roles					1404:1408	significant roles	1392:1408	significant roles	1392:1408	We conclude that Gel proteins play significant roles in structural modification of the fungal cell wall during appressorium-mediated plant infection.
27568483	5	63	theme	vegetative	870:879	arg1	growth					881:886	vegetative growth	870:886	vegetative growth	870:886	We reveal that M. oryzae GH72+ GELs are expressed in spores and during both infective and vegetative growth, but each individual Gel enzymes are dispensable for pathogenicity.
27568483	6	64	theme	polymerization	1086:1099	arg1	degree					1076:1081	a higher degree	1067:1081	a higher degree of polymerization	1067:1099	Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain.
27568483	8	65	theme	cell	1451:1454	arg1	wall					1456:1459	the fungal cell wall	1440:1459	the fungal cell wall	1440:1459	We conclude that Gel proteins play significant roles in structural modification of the fungal cell wall during appressorium-mediated plant infection.
27568483	8	66	theme	Gel	1374:1376	arg1	proteins					1378:1385	Gel proteins	1374:1385	Gel proteins	1374:1385	We conclude that Gel proteins play significant roles in structural modification of the fungal cell wall during appressorium-mediated plant infection.
27568483	5	67	theme	Gel	909:911	arg1	enzymes					913:919	each individual Gel enzymes	893:919	each individual Gel enzymes	893:919	We reveal that M. oryzae GH72+ GELs are expressed in spores and during both infective and vegetative growth, but each individual Gel enzymes are dispensable for pathogenicity.
27568483	1	68	theme	fungal	146:151	arg1	pivotal					161:167	pivotal	161:167	pivotal	161:167	The fungal wall is pivotal for cell shape and function, and in interfacial protection during host infection and environmental challenge.
27568483	1	68	theme	fungal	146:151	arg1	wall					153:156	The fungal wall	142:156	The fungal wall	142:156	The fungal wall is pivotal for cell shape and function, and in interfacial protection during host infection and environmental challenge.
27568483	1	69	theme	interfacial	205:215	arg1	protection					217:226	interfacial protection	205:226	interfacial protection during host infection and environmental challenge	205:276	The fungal wall is pivotal for cell shape and function, and in interfacial protection during host infection and environmental challenge.
27568483	7	70	theme	significant	1168:1178	arg1	differences					1180:1190	significant differences	1168:1190	significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation	1168:1276	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	7	71	theme	phenotype	1249:1257	arg1	patterns					1202:1209	global patterns	1195:1209	global patterns of gene expression, a hyper-branching phenotype and no sporulation	1195:1276	The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues).
27568483	4	72	theme	deletion	629:636	arg1	mutants					638:644	targeted deletion mutants	620:644	targeted deletion mutants	620:644	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	4	72	theme	deletion	629:636	arg1	GH72+					680:684	the GH72+	676:684	the GH72+	676:684	We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+ , which carry a putative carbohydrate-binding module, and the GH72- Gels, without this motif.
27568483	5	73	theme	individual	898:907	arg1	enzymes					913:919	each individual Gel enzymes	893:919	each individual Gel enzymes	893:919	We reveal that M. oryzae GH72+ GELs are expressed in spores and during both infective and vegetative growth, but each individual Gel enzymes are dispensable for pathogenicity.
27568483	3	74	theme	glucan	452:457	arg1	Gels					480:483	Gels	480:483	Gels	480:483	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	3	74	theme	glucan	452:457	arg1	proteins					470:477	glucan elongation proteins	452:477	glucan elongation proteins (Gels)	452:484	We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature.
27568483	6	75	contain	has	1016:1018	arg2	wall					1036:1039	a modified cell wall	1020:1039	a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain	1020:1147	Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain.
27568483	6	75	contain	has	1016:1018	arg1	mutant					1009:1014	a Δgel1Δgel3Δgel4 null mutant	986:1014	a Δgel1Δgel3Δgel4 null mutant	986:1014	Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain.
27568483	5	76	dep	M.	795:796	arg1	oryzae					798:803	oryzae	798:803	oryzae	798:803	We reveal that M. oryzae GH72+ GELs are expressed in spores and during both infective and vegetative growth, but each individual Gel enzymes are dispensable for pathogenicity.
27568483	1	77	from	pivotal	161:167	arg1	protection					217:226	interfacial protection	205:226	interfacial protection during host infection and environmental challenge	205:276	The fungal wall is pivotal for cell shape and function, and in interfacial protection during host infection and environmental challenge.
27568483	2	78	theme	structure	354:362	arg1	description					306:316	the first description	296:316	the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae	296:423	Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae.
24616168	0	0	theme	biomedical	94:103	arg1	applications					105:116	biomedical applications	94:116	biomedical applications	94:116	Nanostructured polymeric coatings based on chitosan and dopamine-modified hyaluronic acid for biomedical applications.
24616168	5	1	theme	nuclear	757:763	arg1	resonance					774:782	nuclear magnetic resonance	757:782	nuclear magnetic resonance	757:782	This conjugate was characterized by distinct techniques, such as nuclear magnetic resonance and ultraviolet spectrophotometry.
24616168	8	2	theme	force	1067:1071	arg1	microscopy					1073:1082	atomic force microscopy	1060:1082	atomic force microscopy	1060:1082	The film surface is characterized by atomic force microscopy and scanning electron microscopy.
24616168	4	3	theme	hyaluronic	575:584	arg1	acid					586:589	dopamine-modified hyaluronic acid	557:589	dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry	557:632	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	4	3	theme	hyaluronic	575:584	arg1	HA-DN					592:596	HA-DN	592:596	HA-DN	592:596	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	5	4	theme	magnetic	765:772	arg1	resonance					774:782	nuclear magnetic resonance	757:782	nuclear magnetic resonance	757:782	This conjugate was characterized by distinct techniques, such as nuclear magnetic resonance and ultraviolet spectrophotometry.
24616168	8	5	theme	atomic	1060:1065	arg1	microscopy					1073:1082	atomic force microscopy	1060:1082	atomic force microscopy	1060:1082	The film surface is characterized by atomic force microscopy and scanning electron microscopy.
24616168	7	6	theme	quartz	994:999	arg1	microbalance					1009:1020	quartz crystal microbalance	994:1020	quartz crystal microbalance	994:1020	The nanostructured films formation is monitored by quartz crystal microbalance.
24616168	4	7	theme	dopamine-modified	557:573	arg1	acid					586:589	dopamine-modified hyaluronic acid	557:589	dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry	557:632	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	4	7	theme	dopamine-modified	557:573	arg1	HA-DN					592:596	HA-DN	592:596	HA-DN	592:596	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	4	8	dep	structure	503:511	arg1	the					499:501	the	499:501	the	499:501	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	7	9	theme	crystal	1001:1007	arg1	microbalance					1009:1020	quartz crystal microbalance	994:1020	quartz crystal microbalance	994:1020	The nanostructured films formation is monitored by quartz crystal microbalance.
24616168	2	10	theme	acid	278:281	arg1	3,4-dihydroxyphenylalanine					283:308	an unusual amino acid 3,4-dihydroxyphenylalanine	261:308	an unusual amino acid 3,4-dihydroxyphenylalanine (known as DOPA)	261:324	These mussel proteins present an unusual amino acid 3,4-dihydroxyphenylalanine (known as DOPA).
24616168	5	11	theme	distinct	728:735	arg1	techniques					737:746	distinct techniques	728:746	distinct techniques	728:746	This conjugate was characterized by distinct techniques, such as nuclear magnetic resonance and ultraviolet spectrophotometry.
24616168	5	11	theme	distinct	728:735	arg1	resonance					774:782	nuclear magnetic resonance	757:782	nuclear magnetic resonance	757:782	This conjugate was characterized by distinct techniques, such as nuclear magnetic resonance and ultraviolet spectrophotometry.
24616168	5	11	theme	distinct	728:735	arg1	spectrophotometry					800:816	ultraviolet spectrophotometry	788:816	ultraviolet spectrophotometry	788:816	This conjugate was characterized by distinct techniques, such as nuclear magnetic resonance and ultraviolet spectrophotometry.
24616168	10	12	with	films	1240:1244	arg1	dopamine					1251:1258	dopamine	1251:1258	dopamine	1251:1258	The adhesion properties are analyzed showing that the nanostructured films with dopamine promote an improved adhesion.
24616168	6	13	theme	Multilayer	819:828	arg1	films					830:834	Multilayer films	819:834	Multilayer films	819:834	Multilayer films are developed based on chitosan and HA-DN to form polymeric coatings using the layer-by-layer methodology.
24616168	2	14	theme	amino	272:276	arg1	3,4-dihydroxyphenylalanine					283:308	an unusual amino acid 3,4-dihydroxyphenylalanine	261:308	an unusual amino acid 3,4-dihydroxyphenylalanine (known as DOPA)	261:324	These mussel proteins present an unusual amino acid 3,4-dihydroxyphenylalanine (known as DOPA).
24616168	9	15	theme	Water	1118:1122	arg1	measurements					1138:1149	Water contact angle measurements	1118:1149	Water contact angle measurements	1118:1149	Water contact angle measurements are also conducted.
24616168	3	16	theme	present	470:476	arg1	groups					463:468	the catechol groups	450:468	the catechol groups present in DOPA	450:484	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	3	17	theme	outstanding	331:341	arg1	properties					352:361	The outstanding adhesive properties	327:361	The outstanding adhesive properties of these materials in the sea harsh conditions	327:408	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	8	18	theme	electron	1097:1104	arg1	microscopy					1106:1115	scanning electron microscopy	1088:1115	scanning electron microscopy	1088:1115	The film surface is characterized by atomic force microscopy and scanning electron microscopy.
24616168	4	19	theme	carbodiimide	611:622	arg1	chemistry					624:632	carbodiimide chemistry	611:632	carbodiimide chemistry	611:632	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	2	20	theme	unusual	264:270	arg1	3,4-dihydroxyphenylalanine					283:308	an unusual amino acid 3,4-dihydroxyphenylalanine	261:308	an unusual amino acid 3,4-dihydroxyphenylalanine (known as DOPA)	261:324	These mussel proteins present an unusual amino acid 3,4-dihydroxyphenylalanine (known as DOPA).
24616168	9	21	theme	contact	1124:1130	arg1	measurements					1138:1149	Water contact angle measurements	1118:1149	Water contact angle measurements	1118:1149	Water contact angle measurements are also conducted.
24616168	3	22	theme	adhesive	343:350	arg1	properties					352:361	The outstanding adhesive properties	327:361	The outstanding adhesive properties of these materials in the sea harsh conditions	327:408	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	8	23	theme	scanning	1088:1095	arg1	microscopy					1106:1115	scanning electron microscopy	1088:1115	scanning electron microscopy	1088:1115	The film surface is characterized by atomic force microscopy and scanning electron microscopy.
24616168	9	24	theme	angle	1132:1136	arg1	measurements					1138:1149	Water contact angle measurements	1118:1149	Water contact angle measurements	1118:1149	Water contact angle measurements are also conducted.
24616168	11	25	theme	distinct	1456:1463	arg1	applications					1476:1487	distinct biomedical applications	1456:1487	distinct biomedical applications	1456:1487	In vitro tests show an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.
24616168	0	26	theme	polymeric	15:23	arg1	coatings					25:32	Nanostructured polymeric coatings	0:32	Nanostructured polymeric coatings	0:32	Nanostructured polymeric coatings based on chitosan and dopamine-modified hyaluronic acid for biomedical applications.
24616168	6	27	theme	polymeric	886:894	arg1	coatings					896:903	polymeric coatings	886:903	polymeric coatings using the layer-by-layer methodology	886:940	Multilayer films are developed based on chitosan and HA-DN to form polymeric coatings using the layer-by-layer methodology.
24616168	3	28	from	present	470:476	arg1	DOPA					481:484	DOPA	481:484	DOPA	481:484	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	11	29	with	films	1384:1388	arg1	groups					1404:1409	catechol groups	1395:1409	catechol groups	1395:1409	In vitro tests show an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.
24616168	0	30	theme	Nanostructured	0:13	arg1	coatings					25:32	Nanostructured polymeric coatings	0:32	Nanostructured polymeric coatings	0:32	Nanostructured polymeric coatings based on chitosan and dopamine-modified hyaluronic acid for biomedical applications.
24616168	4	31	theme	adhesive	538:545	arg1	proteins					547:554	these adhesive proteins	532:554	these adhesive proteins	532:554	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	7	32	theme	nanostructured	947:960	arg1	formation					968:976	The nanostructured films formation	943:976	The nanostructured films formation	943:976	The nanostructured films formation is monitored by quartz crystal microbalance.
24616168	4	33	theme	thin	650:653	arg1	films					685:689	thin and surface-adherent dopamine films	650:689	thin and surface-adherent dopamine films	650:689	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	6	34	dep	developed	840:848	arg1	based					850:854	based	850:854	are developed based on chitosan and HA-DN to form polymeric coatings using the layer-by-layer methodology	836:940	Multilayer films are developed based on chitosan and HA-DN to form polymeric coatings using the layer-by-layer methodology.
24616168	3	35	from	DOPA	481:484	arg1	present					470:476	present	470:476	present	470:476	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	5	36	theme	ultraviolet	788:798	arg1	spectrophotometry					800:816	ultraviolet spectrophotometry	788:816	ultraviolet spectrophotometry	788:816	This conjugate was characterized by distinct techniques, such as nuclear magnetic resonance and ultraviolet spectrophotometry.
24616168	3	37	theme	materials	372:380	arg1	properties					352:361	The outstanding adhesive properties	327:361	The outstanding adhesive properties of these materials in the sea harsh conditions	327:408	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	10	38	theme	adhesion	1175:1182	arg1	properties					1184:1193	The adhesion properties	1171:1193	The adhesion properties	1171:1193	The adhesion properties are analyzed showing that the nanostructured films with dopamine promote an improved adhesion.
24616168	11	39	theme	In	1290:1291	arg1	tests					1299:1303	In vitro tests	1290:1303	In vitro tests	1290:1303	In vitro tests show an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.
24616168	3	40	theme	catechol	454:461	arg1	groups					463:468	the catechol groups	450:468	the catechol groups present in DOPA	450:484	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	4	41	theme	proteins	547:554	arg1	composition					517:527	composition	517:527	composition	517:527	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	4	41	theme	proteins	547:554	arg1	structure					503:511	structure	503:511	structure	503:511	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	8	42	theme	film	1027:1030	arg1	surface					1032:1038	The film surface	1023:1038	The film surface	1023:1038	The film surface is characterized by atomic force microscopy and scanning electron microscopy.
24616168	10	43	theme	improved	1271:1278	arg1	adhesion					1280:1287	an improved adhesion	1268:1287	an improved adhesion	1268:1287	The adhesion properties are analyzed showing that the nanostructured films with dopamine promote an improved adhesion.
24616168	3	44	from	properties	352:361	arg1	conditions					399:408	the sea harsh conditions	385:408	the sea harsh conditions	385:408	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	11	45	theme	biomedical	1465:1474	arg1	applications					1476:1487	distinct biomedical applications	1456:1487	distinct biomedical applications	1456:1487	In vitro tests show an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.
24616168	3	46	theme	sea	389:391	arg1	conditions					399:408	the sea harsh conditions	385:408	the sea harsh conditions	385:408	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	11	47	theme	enhanced	1313:1320	arg1	adhesion					1327:1334	an enhanced cell adhesion	1310:1334	an enhanced cell adhesion	1310:1334	In vitro tests show an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.
24616168	0	48	theme	hyaluronic	74:83	arg1	acid					85:88	dopamine-modified hyaluronic acid	56:88	dopamine-modified hyaluronic acid	56:88	Nanostructured polymeric coatings based on chitosan and dopamine-modified hyaluronic acid for biomedical applications.
24616168	1	49	theme	marine	124:129	arg1	environment					131:141	a marine environment	122:141	a marine environment	122:141	In a marine environment, specific proteins are secreted by mussels and used as a bioglue to stick to a surface.
24616168	3	50	theme	harsh	393:397	arg1	conditions					399:408	the sea harsh conditions	385:408	the sea harsh conditions	385:408	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	11	51	theme	cell	1322:1325	arg1	adhesion					1327:1334	an enhanced cell adhesion	1310:1334	an enhanced cell adhesion	1310:1334	In vitro tests show an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.
24616168	11	52	theme	biomimetic	1373:1382	arg1	films					1384:1388	the biomimetic films	1369:1388	the biomimetic films with catechol groups	1369:1409	In vitro tests show an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.
24616168	11	53	dep	In	1290:1291	arg1	vitro					1293:1297	vitro	1293:1297	vitro	1293:1297	In vitro tests show an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.
24616168	2	54	dep	3,4-dihydroxyphenylalanine	283:308	arg1	known					311:315	known	311:315	known as DOPA	311:323	These mussel proteins present an unusual amino acid 3,4-dihydroxyphenylalanine (known as DOPA).
24616168	7	55	theme	films	962:966	arg1	formation					968:976	The nanostructured films formation	943:976	The nanostructured films formation	943:976	The nanostructured films formation is monitored by quartz crystal microbalance.
24616168	3	56	theme	groups	463:468	arg1	presence					438:445	the presence	434:445	the presence of the catechol groups present in DOPA	434:484	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	11	57	theme	catechol	1395:1402	arg1	groups					1404:1409	catechol groups	1395:1409	catechol groups	1395:1409	In vitro tests show an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.
24616168	1	58	used	used	190:193	arg2	bioglue					200:206	a bioglue	198:206	a bioglue to stick to a surface	198:228	In a marine environment, specific proteins are secreted by mussels and used as a bioglue to stick to a surface.
24616168	1	58	used	used	190:193	arg2	proteins					153:160	specific proteins	144:160	specific proteins	144:160	In a marine environment, specific proteins are secreted by mussels and used as a bioglue to stick to a surface.
24616168	1	59	theme	specific	144:151	arg1	bioglue					200:206	a bioglue	198:206	a bioglue to stick to a surface	198:228	In a marine environment, specific proteins are secreted by mussels and used as a bioglue to stick to a surface.
24616168	1	59	theme	specific	144:151	arg1	proteins					153:160	specific proteins	144:160	specific proteins	144:160	In a marine environment, specific proteins are secreted by mussels and used as a bioglue to stick to a surface.
24616168	2	60	theme	mussel	237:242	arg1	proteins					244:251	These mussel proteins	231:251	These mussel proteins	231:251	These mussel proteins present an unusual amino acid 3,4-dihydroxyphenylalanine (known as DOPA).
24616168	4	61	used	used	637:640	arg2	HA-DN					592:596	HA-DN	592:596	HA-DN	592:596	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	4	61	used	used	637:640	arg2	acid					586:589	dopamine-modified hyaluronic acid	557:589	dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry	557:632	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	4	62	theme	dopamine	676:683	arg1	films					685:689	thin and surface-adherent dopamine films	650:689	thin and surface-adherent dopamine films	650:689	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	6	63	theme	layer-by-layer	915:928	arg1	methodology					930:940	the layer-by-layer methodology	911:940	the layer-by-layer methodology	911:940	Multilayer films are developed based on chitosan and HA-DN to form polymeric coatings using the layer-by-layer methodology.
24616168	0	64	theme	dopamine-modified	56:72	arg1	acid					85:88	dopamine-modified hyaluronic acid	56:88	dopamine-modified hyaluronic acid	56:88	Nanostructured polymeric coatings based on chitosan and dopamine-modified hyaluronic acid for biomedical applications.
24616168	3	65	attach	present	470:476	arg2	groups					463:468	the catechol groups	450:468	the catechol groups present in DOPA	450:484	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	3	65	attach	present	470:476	arg1	DOPA					481:484	DOPA	481:484	DOPA	481:484	The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA.
24616168	4	66	theme	surface-adherent	659:674	arg1	films					685:689	thin and surface-adherent dopamine films	650:689	thin and surface-adherent dopamine films	650:689	Inspired by the structure and composition of these adhesive proteins, dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry is used to form thin and surface-adherent dopamine films.
24616168	10	67	theme	nanostructured	1225:1238	arg1	films					1240:1244	the nanostructured films	1221:1244	the nanostructured films with dopamine	1221:1258	The adhesion properties are analyzed showing that the nanostructured films with dopamine promote an improved adhesion.
29429667	1	0	theme	assisted	253:260	arg1	wet-heating					262:272	traditional and ultrasonic assisted wet-heating	226:272	wet-heating	262:272	Rapeseed protein isolate (RPI) and dextran conjugates were prepared by traditional and ultrasonic assisted wet-heating.
29429667	6	1	theme	electrophoresis	816:830	arg1	results					870:876	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results	774:876	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results	774:876	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	13	2	theme	RPI-dextran	2052:2062	arg1	conjugates					2064:2073	RPI-dextran conjugates	2052:2073	RPI-dextran conjugates	2052:2073	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	10	3	from	increase	1561:1568	arg1	110-20.5 kDa					1581:1592	110-20.5 kDa	1581:1592	110-20.5 kDa	1581:1592	The two treatments also caused molecular weight to become bigger, and ultrasonic treatment had the greatest effect on the increase (68.2%) in 110-20.5 kDa.
29429667	10	4	theme	ultrasonic	1509:1518	arg1	treatment					1520:1528	ultrasonic treatment	1509:1528	ultrasonic treatment	1509:1528	The two treatments also caused molecular weight to become bigger, and ultrasonic treatment had the greatest effect on the increase (68.2%) in 110-20.5 kDa.
29429667	11	5	theme	Structural	1595:1604	arg1	modifications					1606:1618	Structural modifications	1595:1618	Structural modifications of RPI by grafting to dextran	1595:1648	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	6	6	theme	sulfate-polyacrylamide	789:810	arg1	electrophoresis					816:830	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	774:830	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	774:841	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	6	6	theme	sulfate-polyacrylamide	789:810	arg1	SDS-PAGE					833:840	SDS-PAGE	833:840	SDS-PAGE	833:840	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	9	7	theme	surface	1397:1403	arg1	structure					1405:1413	surface structure	1397:1413	surface structure	1397:1413	Graft and ultrasonic treatments both made surface structure looser and more porous.
29429667	6	8	theme	traditional	893:903	arg1	reaction					911:918	traditional graft reaction	893:918	traditional graft reaction	893:918	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	11	9	from	pH	1722:1723	arg1	stability					1785:1793	thermal stability	1777:1793	thermal stability (at temperature 90-100 °C)	1777:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	9	from	pH	1722:1723	arg1	improvements					1657:1668	improvements	1657:1668	improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C)	1657:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	9	from	pH	1722:1723	arg1	stability					1741:1749	emulsion stability	1732:1749	emulsion stability (at pH 4-5 and 9-10)	1732:1770	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	9	from	pH	1722:1723	arg1	activity					1709:1716	emulsifying activity	1697:1716	emulsifying activity (at pH 4-10)	1697:1729	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	9	from	pH	1722:1723	arg1	solubility					1673:1682	solubility	1673:1682	solubility (at pH 5-6)	1673:1694	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	0	10	theme	conjugates	143:152	arg1	structure					63:71	structure	63:71	structure	63:71	Effects of ultrasonic and graft treatments on grafting degree, structure, functionality, and digestibility of rapeseed protein isolate-dextran conjugates.
29429667	0	10	theme	conjugates	143:152	arg1	degree					55:60	degree	55:60	degree	55:60	Effects of ultrasonic and graft treatments on grafting degree, structure, functionality, and digestibility of rapeseed protein isolate-dextran conjugates.
29429667	0	10	theme	conjugates	143:152	arg1	functionality					74:86	functionality	74:86	functionality	74:86	Effects of ultrasonic and graft treatments on grafting degree, structure, functionality, and digestibility of rapeseed protein isolate-dextran conjugates.
29429667	0	10	theme	conjugates	143:152	arg1	digestibility					93:105	digestibility	93:105	digestibility	93:105	Effects of ultrasonic and graft treatments on grafting degree, structure, functionality, and digestibility of rapeseed protein isolate-dextran conjugates.
29429667	8	11	theme	greatest	1255:1262	arg1	impact					1264:1269	the greatest impact	1251:1269	the greatest impact	1251:1269	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	10	12	theme	greatest	1538:1545	arg1	effect					1547:1552	the greatest effect	1534:1552	the greatest effect	1534:1552	The two treatments also caused molecular weight to become bigger, and ultrasonic treatment had the greatest effect on the increase (68.2%) in 110-20.5 kDa.
29429667	2	13	theme	grafting	294:301	arg1	structure					316:324	structure	316:324	structure	316:324	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	2	13	theme	grafting	294:301	arg1	degree					303:308	the grafting degree	290:308	the grafting degree (GD)	290:313	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	2	13	theme	grafting	294:301	arg1	GD					311:312	GD	311:312	GD	311:312	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	2	13	theme	grafting	294:301	arg1	functionality					327:339	functionality	327:339	functionality	327:339	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	2	13	theme	grafting	294:301	arg1	digestibility					346:358	digestibility	346:358	digestibility	346:358	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	1	14	theme	dextran	190:196	arg1	conjugates					198:207	dextran conjugates	190:207	dextran conjugates	190:207	Rapeseed protein isolate (RPI) and dextran conjugates were prepared by traditional and ultrasonic assisted wet-heating.
29429667	11	15	theme	emulsion	1732:1739	arg1	stability					1741:1749	emulsion stability	1732:1749	emulsion stability (at pH 4-5 and 9-10)	1732:1770	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	5	16	theme	conjugates	698:707	arg1	hydrophobicities					678:693	surface hydrophobicities	670:693	surface hydrophobicities of conjugates	670:707	Compared to RPI, surface hydrophobicities of conjugates were significantly decreased by graft and ultrasonic treatments.
29429667	8	17	theme	ultrasonic	1226:1235	arg1	treatment					1237:1245	ultrasonic treatment	1226:1245	ultrasonic treatment	1226:1245	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	18	dep	Fourier	1072:1078	arg1	transform					1080:1088	transform	1080:1088	transform infrared spectrum (FT-IR) and circular dichroism (CD) results	1080:1150	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	7	19	theme	globulin	1039:1046	arg1	subunit					1048:1054	the 12S globulin subunit	1031:1054	the 12S globulin subunit	1031:1054	Both were from the 12S globulin subunit and cruciferin.
29429667	13	20	theme	ultrasonic	1973:1982	arg1	wet-heating					1993:2003	The ultrasonic assisted wet-heating	1969:2003	The ultrasonic assisted wet-heating	1969:2003	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	13	20	theme	ultrasonic	1973:1982	arg1	method					2031:2036	an efficient and safe method	2009:2036	an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal	2009:2126	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	2	21	from	effects	279:285	arg1	structure					316:324	structure	316:324	structure	316:324	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	2	21	from	effects	279:285	arg1	degree					303:308	the grafting degree	290:308	the grafting degree (GD)	290:313	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	2	21	from	effects	279:285	arg1	GD					311:312	GD	311:312	GD	311:312	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	2	21	from	effects	279:285	arg1	functionality					327:339	functionality	327:339	functionality	327:339	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	2	21	from	effects	279:285	arg1	digestibility					346:358	digestibility	346:358	digestibility	346:358	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	11	22	from	pH	1688:1689	arg1	stability					1785:1793	thermal stability	1777:1793	thermal stability (at temperature 90-100 °C)	1777:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	22	from	pH	1688:1689	arg1	improvements					1657:1668	improvements	1657:1668	improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C)	1657:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	22	from	pH	1688:1689	arg1	stability					1741:1749	emulsion stability	1732:1749	emulsion stability (at pH 4-5 and 9-10)	1732:1770	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	22	from	pH	1688:1689	arg1	activity					1709:1716	emulsifying activity	1697:1716	emulsifying activity (at pH 4-10)	1697:1729	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	22	from	pH	1688:1689	arg1	solubility					1673:1682	solubility	1673:1682	solubility (at pH 5-6)	1673:1694	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	9	23	theme	ultrasonic	1365:1374	arg1	treatments					1376:1385	ultrasonic treatments	1365:1385	ultrasonic treatments	1365:1385	Graft and ultrasonic treatments both made surface structure looser and more porous.
29429667	8	24	from	decrease	1278:1285	arg1	β-sheet					1294:1300	the β-sheet	1290:1300	the β-sheet (19.1%)	1290:1308	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	24	from	decrease	1278:1285	arg1	coil					1341:1344	the random coil	1330:1344	the random coil (49.6%)	1330:1352	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	24	from	decrease	1278:1285	arg1	%					1351:1351	49.6%	1347:1351	49.6%	1347:1351	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	24	from	decrease	1278:1285	arg1	%					1307:1307	19.1%	1303:1307	19.1%	1303:1307	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	4	25	theme	optimum	477:483	arg1	conditions					485:494	the optimum conditions	473:494	the optimum conditions (temperature of 90 °C and time of 60 min)	473:536	Under the optimum conditions (temperature of 90 °C and time of 60 min), compared to traditional wet-heating, ultrasonic treatment at 28 kHz significantly increased the GD by 2.12 times.
29429667	11	26	theme	emulsifying	1697:1707	arg1	activity					1709:1716	emulsifying activity	1697:1716	emulsifying activity (at pH 4-10)	1697:1729	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	1	27	theme	protein	164:170	arg1	RPI					181:183	RPI	181:183	RPI	181:183	Rapeseed protein isolate (RPI) and dextran conjugates were prepared by traditional and ultrasonic assisted wet-heating.
29429667	1	27	theme	protein	164:170	arg1	isolate					172:178	Rapeseed protein isolate	155:178	Rapeseed protein isolate (RPI)	155:184	Rapeseed protein isolate (RPI) and dextran conjugates were prepared by traditional and ultrasonic assisted wet-heating.
29429667	11	28	from	9-10	1766:1769	arg1	stability					1785:1793	thermal stability	1777:1793	thermal stability (at temperature 90-100 °C)	1777:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	28	from	9-10	1766:1769	arg1	improvements					1657:1668	improvements	1657:1668	improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C)	1657:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	28	from	9-10	1766:1769	arg1	stability					1741:1749	emulsion stability	1732:1749	emulsion stability (at pH 4-5 and 9-10)	1732:1770	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	28	from	9-10	1766:1769	arg1	activity					1709:1716	emulsifying activity	1697:1716	emulsifying activity (at pH 4-10)	1697:1729	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	28	from	9-10	1766:1769	arg1	solubility					1673:1682	solubility	1673:1682	solubility (at pH 5-6)	1673:1694	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	6	29	theme	assisted	984:991	arg1	one					993:995	the ultrasonic assisted one	969:995	the ultrasonic assisted one	969:995	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	11	30	theme	stability	1785:1793	arg1	improvements					1657:1668	improvements	1657:1668	improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C)	1657:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	2	31	theme	conjugates	363:372	arg1	structure					316:324	structure	316:324	structure	316:324	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	2	31	theme	conjugates	363:372	arg1	degree					303:308	the grafting degree	290:308	the grafting degree (GD)	290:313	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	2	31	theme	conjugates	363:372	arg1	functionality					327:339	functionality	327:339	functionality	327:339	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	2	31	theme	conjugates	363:372	arg1	digestibility					346:358	digestibility	346:358	digestibility	346:358	The effects on the grafting degree (GD), structure, functionality, and digestibility of conjugates were studied.
29429667	13	32	theme	meal	2123:2126	arg1	value					2105:2109	the utilization value	2089:2109	the utilization value of rapeseed meal	2089:2126	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	0	33	theme	ultrasonic	11:20	arg1	treatments					32:41	ultrasonic and graft treatments	11:41	ultrasonic and graft treatments	11:41	Effects of ultrasonic and graft treatments on grafting degree, structure, functionality, and digestibility of rapeseed protein isolate-dextran conjugates.
29429667	0	34	theme	graft	26:30	arg1	treatments					32:41	ultrasonic and graft treatments	11:41	ultrasonic and graft treatments	11:41	Effects of ultrasonic and graft treatments on grafting degree, structure, functionality, and digestibility of rapeseed protein isolate-dextran conjugates.
29429667	13	35	theme	utilization	2093:2103	arg1	value					2105:2109	the utilization value	2089:2109	the utilization value of rapeseed meal	2089:2126	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	8	36	theme	infrared	1090:1097	arg1	FT-IR					1109:1113	FT-IR	1109:1113	FT-IR	1109:1113	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	36	theme	infrared	1090:1097	arg1	spectrum					1099:1106	infrared spectrum	1090:1106	infrared spectrum (FT-IR)	1090:1114	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	4	37	dep	conditions	485:494	arg1	temperature					497:507	temperature	497:507	temperature of 90 °C and time of 60 min	497:535	Under the optimum conditions (temperature of 90 °C and time of 60 min), compared to traditional wet-heating, ultrasonic treatment at 28 kHz significantly increased the GD by 2.12 times.
29429667	6	38	theme	acid	853:856	arg1	composition					858:868	amino acid composition	847:868	amino acid composition	847:868	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	10	39	theme	molecular	1470:1478	arg1	weight					1480:1485	molecular weight	1470:1485	molecular weight to become bigger	1470:1502	The two treatments also caused molecular weight to become bigger, and ultrasonic treatment had the greatest effect on the increase (68.2%) in 110-20.5 kDa.
29429667	11	40	theme	solubility	1673:1682	arg1	improvements					1657:1668	improvements	1657:1668	improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C)	1657:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	1	41	theme	traditional	226:236	arg1	wet-heating					262:272	traditional and ultrasonic assisted wet-heating	226:272	wet-heating	262:272	Rapeseed protein isolate (RPI) and dextran conjugates were prepared by traditional and ultrasonic assisted wet-heating.
29429667	8	42	theme	circular	1120:1127	arg1	CD					1140:1141	CD	1140:1141	CD	1140:1141	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	42	theme	circular	1120:1127	arg1	dichroism					1129:1137	circular dichroism	1120:1137	circular dichroism (CD)	1120:1142	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	1	43	theme	ultrasonic	242:251	arg1	wet-heating					262:272	traditional and ultrasonic assisted wet-heating	226:272	wet-heating	262:272	Rapeseed protein isolate (RPI) and dextran conjugates were prepared by traditional and ultrasonic assisted wet-heating.
29429667	4	44	theme	traditional	551:561	arg1	wet-heating					563:573	traditional wet-heating	551:573	traditional wet-heating	551:573	Under the optimum conditions (temperature of 90 °C and time of 60 min), compared to traditional wet-heating, ultrasonic treatment at 28 kHz significantly increased the GD by 2.12 times.
29429667	11	45	theme	RPI	1623:1625	arg1	modifications					1606:1618	Structural modifications	1595:1618	Structural modifications of RPI by grafting to dextran	1595:1648	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	6	46	theme	gel	812:814	arg1	electrophoresis					816:830	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	774:830	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	774:841	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	6	46	theme	gel	812:814	arg1	SDS-PAGE					833:840	SDS-PAGE	833:840	SDS-PAGE	833:840	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	12	47	theme	ultrasonic	1882:1891	arg1	treatments					1893:1902	ultrasonic treatments	1882:1902	ultrasonic treatments	1882:1902	The digestibility of conjugates was decreased by graft and ultrasonic treatments and the conjugates were mainly digested in the intestinal phase.
29429667	6	48	theme	graft	905:909	arg1	reaction					911:918	traditional graft reaction	893:918	traditional graft reaction	893:918	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	8	49	theme	graft	1164:1168	arg1	treatment					1170:1178	graft treatment	1164:1178	graft treatment	1164:1178	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	12	50	theme	conjugates	1844:1853	arg1	digestibility					1827:1839	The digestibility	1823:1839	The digestibility of conjugates	1823:1853	The digestibility of conjugates was decreased by graft and ultrasonic treatments and the conjugates were mainly digested in the intestinal phase.
29429667	6	51	theme	ultrasonic	973:982	arg1	one					993:995	the ultrasonic assisted one	969:995	the ultrasonic assisted one	969:995	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	8	52	contain	had	1247:1249	arg1	treatment					1237:1245	ultrasonic treatment	1226:1245	ultrasonic treatment	1226:1245	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	52	contain	had	1247:1249	arg2	impact					1264:1269	the greatest impact	1251:1269	the greatest impact	1251:1269	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	0	53	theme	rapeseed	110:117	arg1	conjugates					143:152	rapeseed protein isolate-dextran conjugates	110:152	rapeseed protein isolate-dextran conjugates	110:152	Effects of ultrasonic and graft treatments on grafting degree, structure, functionality, and digestibility of rapeseed protein isolate-dextran conjugates.
29429667	0	54	theme	isolate-dextran	127:141	arg1	conjugates					143:152	rapeseed protein isolate-dextran conjugates	110:152	rapeseed protein isolate-dextran conjugates	110:152	Effects of ultrasonic and graft treatments on grafting degree, structure, functionality, and digestibility of rapeseed protein isolate-dextran conjugates.
29429667	13	55	theme	efficient	2012:2020	arg1	wet-heating					1993:2003	The ultrasonic assisted wet-heating	1969:2003	The ultrasonic assisted wet-heating	1969:2003	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	13	55	theme	efficient	2012:2020	arg1	method					2031:2036	an efficient and safe method	2009:2036	an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal	2009:2126	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	4	56	from	28 kHz	600:605	arg1	treatment					587:595	ultrasonic treatment	576:595	ultrasonic treatment at 28 kHz	576:605	Under the optimum conditions (temperature of 90 °C and time of 60 min), compared to traditional wet-heating, ultrasonic treatment at 28 kHz significantly increased the GD by 2.12 times.
29429667	8	57	theme	secondary	1202:1210	arg1	structure					1212:1220	secondary structure	1202:1220	secondary structure	1202:1220	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	11	58	theme	stability	1741:1749	arg1	improvements					1657:1668	improvements	1657:1668	improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C)	1657:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	7	59	theme	12S	1035:1037	arg1	subunit					1048:1054	the 12S globulin subunit	1031:1054	the 12S globulin subunit	1031:1054	Both were from the 12S globulin subunit and cruciferin.
29429667	13	60	theme	safe	2026:2029	arg1	wet-heating					1993:2003	The ultrasonic assisted wet-heating	1969:2003	The ultrasonic assisted wet-heating	1969:2003	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	13	60	theme	safe	2026:2029	arg1	method					2031:2036	an efficient and safe method	2009:2036	an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal	2009:2126	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	5	61	theme	surface	670:676	arg1	hydrophobicities					678:693	surface hydrophobicities	670:693	surface hydrophobicities of conjugates	670:707	Compared to RPI, surface hydrophobicities of conjugates were significantly decreased by graft and ultrasonic treatments.
29429667	11	62	from	90-100 °C	1811:1819	arg1	stability					1785:1793	thermal stability	1777:1793	thermal stability (at temperature 90-100 °C)	1777:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	62	from	90-100 °C	1811:1819	arg1	improvements					1657:1668	improvements	1657:1668	improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C)	1657:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	62	from	90-100 °C	1811:1819	arg1	stability					1741:1749	emulsion stability	1732:1749	emulsion stability (at pH 4-5 and 9-10)	1732:1770	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	62	from	90-100 °C	1811:1819	arg1	activity					1709:1716	emulsifying activity	1697:1716	emulsifying activity (at pH 4-10)	1697:1729	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	62	from	90-100 °C	1811:1819	arg1	solubility					1673:1682	solubility	1673:1682	solubility (at pH 5-6)	1673:1694	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	12	63	theme	intestinal	1951:1960	arg1	phase					1962:1966	the intestinal phase	1947:1966	the intestinal phase	1947:1966	The digestibility of conjugates was decreased by graft and ultrasonic treatments and the conjugates were mainly digested in the intestinal phase.
29429667	13	64	theme	assisted	1984:1991	arg1	wet-heating					1993:2003	The ultrasonic assisted wet-heating	1969:2003	The ultrasonic assisted wet-heating	1969:2003	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	13	64	theme	assisted	1984:1991	arg1	method					2031:2036	an efficient and safe method	2009:2036	an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal	2009:2126	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	11	65	from	improvements	1657:1668	arg1	pH					1722:1723	pH 4-10	1722:1728	pH 4-10	1722:1728	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	65	from	improvements	1657:1668	arg1	9-10					1766:1769	9-10	1766:1769	9-10	1766:1769	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	65	from	improvements	1657:1668	arg1	pH					1688:1689	pH 5-6	1688:1693	pH 5-6	1688:1693	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	65	from	improvements	1657:1668	arg1	90-100 °C					1811:1819	temperature 90-100 °C	1799:1819	temperature 90-100 °C	1799:1819	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	65	from	improvements	1657:1668	arg1	pH					1755:1756	pH 4-5 and 9-10	1755:1769	pH	1755:1756	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	5	66	theme	ultrasonic	751:760	arg1	treatments					762:771	ultrasonic treatments	751:771	ultrasonic treatments	751:771	Compared to RPI, surface hydrophobicities of conjugates were significantly decreased by graft and ultrasonic treatments.
29429667	1	67	theme	Rapeseed	155:162	arg1	RPI					181:183	RPI	181:183	RPI	181:183	Rapeseed protein isolate (RPI) and dextran conjugates were prepared by traditional and ultrasonic assisted wet-heating.
29429667	1	67	theme	Rapeseed	155:162	arg1	isolate					172:178	Rapeseed protein isolate	155:178	Rapeseed protein isolate (RPI)	155:184	Rapeseed protein isolate (RPI) and dextran conjugates were prepared by traditional and ultrasonic assisted wet-heating.
29429667	6	68	theme	only	1006:1009	arg1	Cys					1011:1013	only Cys	1006:1013	only Cys	1006:1013	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	11	69	theme	activity	1709:1716	arg1	improvements					1657:1668	improvements	1657:1668	improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C)	1657:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	3	70	theme	time	427:430	arg1	all					432:434	time all	427:434	time all	427:434	Ultrasonic frequency, temperature, and time all significantly affected the GD.
29429667	3	71	theme	Ultrasonic	388:397	arg1	frequency					399:407	Ultrasonic frequency	388:407	Ultrasonic frequency	388:407	Ultrasonic frequency, temperature, and time all significantly affected the GD.
29429667	4	72	theme	60 min	530:535	arg1	time					522:525	time	522:525	time	522:525	Under the optimum conditions (temperature of 90 °C and time of 60 min), compared to traditional wet-heating, ultrasonic treatment at 28 kHz significantly increased the GD by 2.12 times.
29429667	4	72	theme	60 min	530:535	arg1	90 °C					512:516	90 °C	512:516	90 °C	512:516	Under the optimum conditions (temperature of 90 °C and time of 60 min), compared to traditional wet-heating, ultrasonic treatment at 28 kHz significantly increased the GD by 2.12 times.
29429667	11	73	from	pH	1755:1756	arg1	stability					1785:1793	thermal stability	1777:1793	thermal stability (at temperature 90-100 °C)	1777:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	73	from	pH	1755:1756	arg1	improvements					1657:1668	improvements	1657:1668	improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C)	1657:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	73	from	pH	1755:1756	arg1	stability					1741:1749	emulsion stability	1732:1749	emulsion stability (at pH 4-5 and 9-10)	1732:1770	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	73	from	pH	1755:1756	arg1	activity					1709:1716	emulsifying activity	1697:1716	emulsifying activity (at pH 4-10)	1697:1729	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	11	73	from	pH	1755:1756	arg1	solubility					1673:1682	solubility	1673:1682	solubility (at pH 5-6)	1673:1694	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	8	74	theme	random	1334:1339	arg1	coil					1341:1344	the random coil	1330:1344	the random coil (49.6%)	1330:1352	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	74	theme	random	1334:1339	arg1	%					1351:1351	49.6%	1347:1351	49.6%	1347:1351	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	11	75	theme	thermal	1777:1783	arg1	stability					1785:1793	thermal stability	1777:1793	thermal stability (at temperature 90-100 °C)	1777:1820	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	10	76	contain	had	1530:1532	arg1	treatment					1520:1528	ultrasonic treatment	1509:1528	ultrasonic treatment	1509:1528	The two treatments also caused molecular weight to become bigger, and ultrasonic treatment had the greatest effect on the increase (68.2%) in 110-20.5 kDa.
29429667	10	76	contain	had	1530:1532	arg2	effect					1547:1552	the greatest effect	1534:1552	the greatest effect	1534:1552	The two treatments also caused molecular weight to become bigger, and ultrasonic treatment had the greatest effect on the increase (68.2%) in 110-20.5 kDa.
29429667	0	77	theme	treatments	32:41	arg1	Effects					0:6	Effects	0:6	Effects of ultrasonic and graft treatments on grafting degree, structure, functionality, and digestibility of rapeseed protein isolate-dextran conjugates.	0:153	Effects of ultrasonic and graft treatments on grafting degree, structure, functionality, and digestibility of rapeseed protein isolate-dextran conjugates.
29429667	8	78	theme	spectrum	1099:1106	arg1	results					1144:1150	infrared spectrum (FT-IR) and circular dichroism (CD) results	1090:1150	infrared spectrum (FT-IR) and circular dichroism (CD) results	1090:1150	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	4	79	theme	90 °C	512:516	arg1	temperature					497:507	temperature	497:507	temperature of 90 °C and time of 60 min	497:535	Under the optimum conditions (temperature of 90 °C and time of 60 min), compared to traditional wet-heating, ultrasonic treatment at 28 kHz significantly increased the GD by 2.12 times.
29429667	11	80	theme	temperature	1799:1809	arg1	90-100 °C					1811:1819	temperature 90-100 °C	1799:1819	temperature 90-100 °C	1799:1819	Structural modifications of RPI by grafting to dextran caused improvements of solubility (at pH 5-6), emulsifying activity (at pH 4-10), emulsion stability (at pH 4-5 and 9-10), and thermal stability (at temperature 90-100 °C).
29429667	6	81	theme	composition	858:868	arg1	results					870:876	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results	774:876	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results	774:876	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	0	82	theme	protein	119:125	arg1	conjugates					143:152	rapeseed protein isolate-dextran conjugates	110:152	rapeseed protein isolate-dextran conjugates	110:152	Effects of ultrasonic and graft treatments on grafting degree, structure, functionality, and digestibility of rapeseed protein isolate-dextran conjugates.
29429667	4	83	theme	time	522:525	arg1	temperature					497:507	temperature	497:507	temperature of 90 °C and time of 60 min	497:535	Under the optimum conditions (temperature of 90 °C and time of 60 min), compared to traditional wet-heating, ultrasonic treatment at 28 kHz significantly increased the GD by 2.12 times.
29429667	13	84	theme	rapeseed	2114:2121	arg1	meal					2123:2126	rapeseed meal	2114:2126	rapeseed meal	2114:2126	The ultrasonic assisted wet-heating was an efficient and safe method for producing RPI-dextran conjugates and improving the utilization value of rapeseed meal.
29429667	6	85	theme	amino	847:851	arg1	composition					858:868	amino acid composition	847:868	amino acid composition	847:868	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition results confirmed that traditional graft reaction involved cysteine (Cys) and lysine (Lys) whereas the ultrasonic assisted one involved only Cys.
29429667	4	86	theme	ultrasonic	576:585	arg1	treatment					587:595	ultrasonic treatment	576:595	ultrasonic treatment at 28 kHz	576:605	Under the optimum conditions (temperature of 90 °C and time of 60 min), compared to traditional wet-heating, ultrasonic treatment at 28 kHz significantly increased the GD by 2.12 times.
29429667	8	87	from	increase	1318:1325	arg1	β-sheet					1294:1300	the β-sheet	1290:1300	the β-sheet (19.1%)	1290:1308	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	87	from	increase	1318:1325	arg1	coil					1341:1344	the random coil	1330:1344	the random coil (49.6%)	1330:1352	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	87	from	increase	1318:1325	arg1	%					1351:1351	49.6%	1347:1351	49.6%	1347:1351	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	87	from	increase	1318:1325	arg1	%					1307:1307	19.1%	1303:1307	19.1%	1303:1307	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
29429667	8	88	theme	dichroism	1129:1137	arg1	results					1144:1150	infrared spectrum (FT-IR) and circular dichroism (CD) results	1090:1150	infrared spectrum (FT-IR) and circular dichroism (CD) results	1090:1150	Fourier transform infrared spectrum (FT-IR) and circular dichroism (CD) results showed that graft treatment significantly changed secondary structure and ultrasonic treatment had the greatest impact on the decrease in the β-sheet (19.1%) and the increase in the random coil (49.6%).
26877003	0	0	theme	nanocomposites	87:100	arg1	phytosynthesis					52:65	in situ phytosynthesis	44:65	in situ phytosynthesis of star-like Ag/ZnO nanocomposites	44:100	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	6	1	theme	zinc	1095:1098	arg1	precursors					1067:1076	precursors	1067:1076	precursors: silver nitrate, zinc acetate and Keliab solution	1067:1126	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	1	theme	zinc	1095:1098	arg1	acetate					1100:1106	zinc acetate	1095:1106	zinc acetate	1095:1106	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	4	2	theme	cotton	784:789	arg1	fabric					791:796	the cotton fabric	780:796	the cotton fabric	780:796	Various analytical techniques were employed to confirm Ag/ZnO nanocomposites on the cotton fabric.
26877003	0	3	theme	Ag/ZnO	80:85	arg1	nanocomposites					87:100	star-like Ag/ZnO nanocomposites	70:100	star-like Ag/ZnO nanocomposites	70:100	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	5	4	dep	emission	911:918	arg1	FE-SEM					954:959	FE-SEM	954:959	FE-SEM	954:959	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	5	4	dep	emission	911:918	arg1	SEM					965:967	SEM	965:967	SEM	965:967	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	5	5	theme	X-ray	971:975	arg1	XRD					990:992	XRD	990:992	XRD	990:992	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	5	5	theme	X-ray	971:975	arg1	diffraction					977:987	X-ray diffraction	971:987	X-ray diffraction (XRD)	971:993	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	8	6	theme	optimized	1480:1488	arg1	conditions					1490:1499	optimized conditions	1480:1499	optimized conditions	1480:1499	The prepared sample in optimized conditions showed good antibacterial activities against Staphylococcus aureus and Escherichia coli with enhanced fabric tensile strength.
26877003	3	7	theme	intermediates	597:609	arg1	ions					611:614	intermediates ions	597:614	intermediates ions	597:614	The intermolecular dehydrolysis of intermediates ions under thermal and alkaline conditions leads to formation of Ag/ZnO heterostructure.
26877003	5	8	theme	X-ray	1018:1022	arg1	EDX					1038:1040	EDX	1038:1040	EDX	1038:1040	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	5	8	theme	X-ray	1018:1022	arg1	spectroscopy					1024:1035	energy dispersive X-ray spectroscopy	1000:1035	energy dispersive X-ray spectroscopy (EDX)	1000:1041	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	1	9	theme	star-like	287:295	arg1	nanocomposites					304:317	star-like Ag/ZnO nanocomposites	287:317	star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab	287:382	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	6	10	dep	precursors	1067:1076	arg1	precursors					1067:1076	precursors	1067:1076	precursors: silver nitrate, zinc acetate and Keliab solution	1067:1126	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	10	dep	precursors	1067:1076	arg1	nitrate					1086:1092	silver nitrate	1079:1092	silver nitrate	1079:1092	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	10	dep	precursors	1067:1076	arg1	acetate					1100:1106	zinc acetate	1095:1106	zinc acetate	1095:1106	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	10	dep	precursors	1067:1076	arg1	solution					1119:1126	Keliab solution	1112:1126	Keliab solution	1112:1126	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	11	theme	central	1256:1262	arg1	CCD					1282:1284	CCD	1282:1284	CCD	1282:1284	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	11	theme	central	1256:1262	arg1	design					1274:1279	central composite design	1256:1279	central composite design (CCD)	1256:1285	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	3	12	theme	ions	611:614	arg1	dehydrolysis					581:592	The intermolecular dehydrolysis	562:592	The intermolecular dehydrolysis of intermediates ions under thermal and alkaline conditions	562:652	The intermolecular dehydrolysis of intermediates ions under thermal and alkaline conditions leads to formation of Ag/ZnO heterostructure.
26877003	1	13	theme	Ag/ZnO	297:302	arg1	nanocomposites					304:317	star-like Ag/ZnO nanocomposites	287:317	star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab	287:382	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	5	14	theme	crystal	823:829	arg1	phase					831:835	crystal phase	823:835	crystal phase	823:835	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	1	15	dep	plants	360:365	arg1	Keliab					377:382	so-called Keliab	367:382	Seidlitzia rosmarinus plants so-called Keliab	338:382	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	5	16	theme	scanning	924:931	arg1	microscopy					942:951	field emission and scanning electron microscopy	905:951	microscopy	942:951	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	4	17	theme	Various	700:706	arg1	techniques					719:728	Various analytical techniques	700:728	Various analytical techniques	700:728	Various analytical techniques were employed to confirm Ag/ZnO nanocomposites on the cotton fabric.
26877003	8	18	theme	prepared	1461:1468	arg1	sample					1470:1475	The prepared sample	1457:1475	The prepared sample in optimized conditions	1457:1499	The prepared sample in optimized conditions showed good antibacterial activities against Staphylococcus aureus and Escherichia coli with enhanced fabric tensile strength.
26877003	6	19	theme	Keliab	1112:1117	arg1	precursors					1067:1076	precursors	1067:1076	precursors: silver nitrate, zinc acetate and Keliab solution	1067:1126	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	19	theme	Keliab	1112:1117	arg1	solution					1119:1126	Keliab solution	1112:1126	Keliab solution	1112:1126	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	5	20	theme	treated	867:873	arg1	fabrics					875:881	the treated fabrics	863:881	the treated fabrics	863:881	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	2	21	theme	groups	554:559	arg1	activation					531:540	activation	531:540	activation of hydroxyl groups	531:559	This is provided alkali media as a vital condition for synthesis of nanocomposites, further increasing the reduce-ability of cellulosic chains by activation of hydroxyl groups.
26877003	2	22	theme	chains	521:526	arg1	reduce-ability					492:505	the reduce-ability	488:505	the reduce-ability of cellulosic chains	488:526	This is provided alkali media as a vital condition for synthesis of nanocomposites, further increasing the reduce-ability of cellulosic chains by activation of hydroxyl groups.
26877003	4	23	theme	analytical	708:717	arg1	techniques					719:728	Various analytical techniques	700:728	Various analytical techniques	700:728	Various analytical techniques were employed to confirm Ag/ZnO nanocomposites on the cotton fabric.
26877003	2	24	theme	vital	420:424	arg1	condition					426:434	a vital condition	418:434	a vital condition for synthesis of nanocomposites	418:466	This is provided alkali media as a vital condition for synthesis of nanocomposites, further increasing the reduce-ability of cellulosic chains by activation of hydroxyl groups.
26877003	2	24	theme	vital	420:424	arg1	This					385:388	This	385:388	This	385:388	This is provided alkali media as a vital condition for synthesis of nanocomposites, further increasing the reduce-ability of cellulosic chains by activation of hydroxyl groups.
26877003	2	24	theme	vital	420:424	arg1	media					409:413	alkali media	402:413	alkali media	402:413	This is provided alkali media as a vital condition for synthesis of nanocomposites, further increasing the reduce-ability of cellulosic chains by activation of hydroxyl groups.
26877003	0	25	theme	active	11:16	arg1	fabric					29:34	Nano-photo active cellulosic fabric	0:34	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.	0:187	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	6	26	from	influence	1054:1062	arg1	attributes					1131:1140	attributes	1131:1140	attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability	1131:1233	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	2	27	theme	hydroxyl	545:552	arg1	groups					554:559	hydroxyl groups	545:559	hydroxyl groups	545:559	This is provided alkali media as a vital condition for synthesis of nanocomposites, further increasing the reduce-ability of cellulosic chains by activation of hydroxyl groups.
26877003	4	28	theme	Ag/ZnO	755:760	arg1	nanocomposites					762:775	Ag/ZnO nanocomposites	755:775	Ag/ZnO nanocomposites	755:775	Various analytical techniques were employed to confirm Ag/ZnO nanocomposites on the cotton fabric.
26877003	0	29	theme	Nano-photo	0:9	arg1	fabric					29:34	Nano-photo active cellulosic fabric	0:34	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.	0:187	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	1	30	theme	nanocomposites	304:317	arg1	phytosynthesis					269:282	in situ phytosynthesis	261:282	in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab	261:382	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	3	31	theme	alkaline	634:641	arg1	conditions					643:652	thermal and alkaline conditions	622:652	conditions	643:652	The intermolecular dehydrolysis of intermediates ions under thermal and alkaline conditions leads to formation of Ag/ZnO heterostructure.
26877003	1	32	theme	nano-photo	204:213	arg1	fabric					233:238	nano-photo active cellulosic fabric	204:238	nano-photo active cellulosic fabric	204:238	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	5	33	theme	field	905:909	arg1	emission					911:918	field emission and scanning electron microscopy	905:951	emission	911:918	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	0	34	theme	attributes	137:146	arg1	Investigation					103:115	Investigation	103:115	Investigation	103:115	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	0	34	theme	attributes	137:146	arg1	optimization					121:132	optimization	121:132	optimization	121:132	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	8	35	theme	antibacterial	1513:1525	arg1	activities					1527:1536	good antibacterial activities	1508:1536	good antibacterial activities against Staphylococcus aureus and Escherichia coli	1508:1587	The prepared sample in optimized conditions showed good antibacterial activities against Staphylococcus aureus and Escherichia coli with enhanced fabric tensile strength.
26877003	5	36	theme	dispersive	1007:1016	arg1	EDX					1038:1040	EDX	1038:1040	EDX	1038:1040	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	5	36	theme	dispersive	1007:1016	arg1	spectroscopy					1024:1035	energy dispersive X-ray spectroscopy	1000:1035	energy dispersive X-ray spectroscopy (EDX)	1000:1041	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	1	37	theme	active	215:220	arg1	fabric					233:238	nano-photo active cellulosic fabric	204:238	nano-photo active cellulosic fabric	204:238	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	0	38	theme	cellulosic	18:27	arg1	fabric					29:34	Nano-photo active cellulosic fabric	0:34	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.	0:187	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	5	39	theme	energy	1000:1005	arg1	EDX					1038:1040	EDX	1038:1040	EDX	1038:1040	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	5	39	theme	energy	1000:1005	arg1	spectroscopy					1024:1035	energy dispersive X-ray spectroscopy	1000:1035	energy dispersive X-ray spectroscopy (EDX)	1000:1041	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	1	40	dep	in	261:262	arg1	situ					264:267	situ	264:267	situ	264:267	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	3	41	theme	thermal	622:628	arg1	conditions					643:652	thermal and alkaline conditions	622:652	conditions	643:652	The intermolecular dehydrolysis of intermediates ions under thermal and alkaline conditions leads to formation of Ag/ZnO heterostructure.
26877003	1	42	theme	cellulosic	222:231	arg1	fabric					233:238	nano-photo active cellulosic fabric	204:238	nano-photo active cellulosic fabric	204:238	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	0	43	theme	in	44:45	arg1	phytosynthesis					52:65	in situ phytosynthesis	44:65	in situ phytosynthesis of star-like Ag/ZnO nanocomposites	44:100	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	7	44	theme	self-cleaning	1325:1337	arg1	activities					1339:1348	self-cleaning activities	1325:1348	self-cleaning activities	1325:1348	The treated cotton samples exhibited self-cleaning activities through methylene blue degradation under day-light exposure along with improved wettability and whiteness.
26877003	8	45	theme	good	1508:1511	arg1	activities					1527:1536	good antibacterial activities	1508:1536	good antibacterial activities against Staphylococcus aureus and Escherichia coli	1508:1587	The prepared sample in optimized conditions showed good antibacterial activities against Staphylococcus aureus and Escherichia coli with enhanced fabric tensile strength.
26877003	7	46	theme	improved	1421:1428	arg1	wettability					1430:1440	improved wettability	1421:1440	improved wettability	1421:1440	The treated cotton samples exhibited self-cleaning activities through methylene blue degradation under day-light exposure along with improved wettability and whiteness.
26877003	5	47	theme	electron	933:940	arg1	microscopy					942:951	field emission and scanning electron microscopy	905:951	microscopy	942:951	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	2	48	theme	cellulosic	510:519	arg1	chains					521:526	cellulosic chains	510:526	cellulosic chains	510:526	This is provided alkali media as a vital condition for synthesis of nanocomposites, further increasing the reduce-ability of cellulosic chains by activation of hydroxyl groups.
26877003	5	49	theme	surface	803:809	arg1	morphology					811:820	surface morphology	803:820	surface morphology	803:820	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	8	50	theme	enhanced	1594:1601	arg1	strength					1618:1625	enhanced fabric tensile strength	1594:1625	enhanced fabric tensile strength	1594:1625	The prepared sample in optimized conditions showed good antibacterial activities against Staphylococcus aureus and Escherichia coli with enhanced fabric tensile strength.
26877003	6	51	theme	precursors	1067:1076	arg1	influence					1054:1062	influence	1054:1062	influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability	1054:1233	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	7	52	theme	day-light	1391:1399	arg1	exposure					1401:1408	day-light exposure	1391:1408	day-light exposure along with improved wettability and whiteness	1391:1454	The treated cotton samples exhibited self-cleaning activities through methylene blue degradation under day-light exposure along with improved wettability and whiteness.
26877003	5	53	theme	fabrics	875:881	arg1	morphology					811:820	surface morphology	803:820	surface morphology	803:820	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	5	53	theme	fabrics	875:881	arg1	phase					831:835	crystal phase	823:835	crystal phase	823:835	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	5	53	theme	fabrics	875:881	arg1	structure					850:858	chemical structure	841:858	chemical structure	841:858	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	1	54	theme	Seidlitzia	338:347	arg1	plants					360:365	Seidlitzia rosmarinus plants so-called Keliab	338:382	Seidlitzia rosmarinus plants so-called Keliab	338:382	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	7	55	theme	cotton	1300:1305	arg1	samples					1307:1313	The treated cotton samples	1288:1313	The treated cotton samples	1288:1313	The treated cotton samples exhibited self-cleaning activities through methylene blue degradation under day-light exposure along with improved wettability and whiteness.
26877003	0	56	dep	in	44:45	arg1	situ					47:50	situ	47:50	situ	47:50	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	7	57	theme	blue	1368:1371	arg1	degradation					1373:1383	methylene blue degradation	1358:1383	methylene blue degradation under day-light exposure along with improved wettability and whiteness	1358:1454	The treated cotton samples exhibited self-cleaning activities through methylene blue degradation under day-light exposure along with improved wettability and whiteness.
26877003	1	58	theme	rosmarinus	349:358	arg1	plants					360:365	Seidlitzia rosmarinus plants so-called Keliab	338:382	Seidlitzia rosmarinus plants so-called Keliab	338:382	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	0	59	theme	photocatalytic	164:177	arg1	activity					179:186	photocatalytic activity	164:186	photocatalytic activity	164:186	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	8	60	theme	tensile	1610:1616	arg1	strength					1618:1625	enhanced fabric tensile strength	1594:1625	enhanced fabric tensile strength	1594:1625	The prepared sample in optimized conditions showed good antibacterial activities against Staphylococcus aureus and Escherichia coli with enhanced fabric tensile strength.
26877003	8	61	theme	fabric	1603:1608	arg1	strength					1618:1625	enhanced fabric tensile strength	1594:1625	enhanced fabric tensile strength	1594:1625	The prepared sample in optimized conditions showed good antibacterial activities against Staphylococcus aureus and Escherichia coli with enhanced fabric tensile strength.
26877003	7	62	theme	treated	1292:1298	arg1	samples					1307:1313	The treated cotton samples	1288:1313	The treated cotton samples	1288:1313	The treated cotton samples exhibited self-cleaning activities through methylene blue degradation under day-light exposure along with improved wettability and whiteness.
26877003	1	63	theme	plants	360:365	arg1	ashes					329:333	the ashes	325:333	the ashes of Seidlitzia rosmarinus plants so-called Keliab	325:382	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	5	64	dep	morphology	811:820	arg1	The					799:801	The	799:801	The	799:801	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	3	65	theme	heterostructure	683:697	arg1	formation					663:671	formation	663:671	formation of Ag/ZnO heterostructure	663:697	The intermolecular dehydrolysis of intermediates ions under thermal and alkaline conditions leads to formation of Ag/ZnO heterostructure.
26877003	0	66	dep	fabric	29:34	arg1	Investigation					103:115	Investigation	103:115	Investigation	103:115	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	0	66	dep	fabric	29:34	arg1	optimization					121:132	optimization	121:132	optimization	121:132	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	6	67	theme	silver	1079:1084	arg1	precursors					1067:1076	precursors	1067:1076	precursors: silver nitrate, zinc acetate and Keliab solution	1067:1126	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	67	theme	silver	1079:1084	arg1	nitrate					1086:1092	silver nitrate	1079:1092	silver nitrate	1079:1092	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	68	theme	composite	1264:1272	arg1	CCD					1282:1284	CCD	1282:1284	CCD	1282:1284	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	68	theme	composite	1264:1272	arg1	design					1274:1279	central composite design	1256:1279	central composite design (CCD)	1256:1285	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	3	69	theme	intermolecular	566:579	arg1	dehydrolysis					581:592	The intermolecular dehydrolysis	562:592	The intermolecular dehydrolysis of intermediates ions under thermal and alkaline conditions	562:652	The intermolecular dehydrolysis of intermediates ions under thermal and alkaline conditions leads to formation of Ag/ZnO heterostructure.
26877003	0	70	theme	star-like	70:78	arg1	nanocomposites					87:100	star-like Ag/ZnO nanocomposites	70:100	star-like Ag/ZnO nanocomposites	70:100	Nano-photo active cellulosic fabric through in situ phytosynthesis of star-like Ag/ZnO nanocomposites: Investigation and optimization of attributes associated with photocatalytic activity.
26877003	8	71	from	sample	1470:1475	arg1	conditions					1490:1499	optimized conditions	1480:1499	optimized conditions	1480:1499	The prepared sample in optimized conditions showed good antibacterial activities against Staphylococcus aureus and Escherichia coli with enhanced fabric tensile strength.
26877003	2	72	theme	nanocomposites	453:466	arg1	synthesis					440:448	synthesis	440:448	synthesis of nanocomposites	440:466	This is provided alkali media as a vital condition for synthesis of nanocomposites, further increasing the reduce-ability of cellulosic chains by activation of hydroxyl groups.
26877003	6	73	theme	photocatalytic	1158:1171	arg1	wettability					1223:1233	wettability	1223:1233	wettability	1223:1233	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	73	theme	photocatalytic	1158:1171	arg1	whiteness					1209:1217	whiteness	1209:1217	whiteness	1209:1217	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	73	theme	photocatalytic	1158:1171	arg1	self-cleaning					1194:1206	self-cleaning	1194:1206	self-cleaning	1194:1206	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	6	73	theme	photocatalytic	1158:1171	arg1	activities					1173:1182	photocatalytic activities	1158:1182	photocatalytic activities including self-cleaning, whiteness and wettability	1158:1233	Moreover, influence of precursors: silver nitrate, zinc acetate and Keliab solution on attributes associated with photocatalytic activities including self-cleaning, whiteness and wettability was investigated via central composite design (CCD).
26877003	1	74	theme	in	261:262	arg1	phytosynthesis					269:282	in situ phytosynthesis	261:282	in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab	261:382	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	5	75	theme	chemical	841:848	arg1	structure					850:858	chemical structure	841:858	chemical structure	841:858	The surface morphology, crystal phase and chemical structure of the treated fabrics were characterized by field emission and scanning electron microscopy (FE-SEM and SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX).
26877003	1	76	theme	so-called	367:375	arg1	Keliab					377:382	so-called Keliab	367:382	Seidlitzia rosmarinus plants so-called Keliab	338:382	In this study, nano-photo active cellulosic fabric was prepared through in situ phytosynthesis of star-like Ag/ZnO nanocomposites using the ashes of Seidlitzia rosmarinus plants so-called Keliab.
26877003	3	77	theme	Ag/ZnO	676:681	arg1	heterostructure					683:697	Ag/ZnO heterostructure	676:697	Ag/ZnO heterostructure	676:697	The intermolecular dehydrolysis of intermediates ions under thermal and alkaline conditions leads to formation of Ag/ZnO heterostructure.
26877003	2	78	theme	alkali	402:407	arg1	condition					426:434	a vital condition	418:434	a vital condition for synthesis of nanocomposites	418:466	This is provided alkali media as a vital condition for synthesis of nanocomposites, further increasing the reduce-ability of cellulosic chains by activation of hydroxyl groups.
26877003	2	78	theme	alkali	402:407	arg1	This					385:388	This	385:388	This	385:388	This is provided alkali media as a vital condition for synthesis of nanocomposites, further increasing the reduce-ability of cellulosic chains by activation of hydroxyl groups.
26877003	2	78	theme	alkali	402:407	arg1	media					409:413	alkali media	402:413	alkali media	402:413	This is provided alkali media as a vital condition for synthesis of nanocomposites, further increasing the reduce-ability of cellulosic chains by activation of hydroxyl groups.
24661867	0	0	theme	phosphated	89:98	arg1	monolith					133:140	a phosphated β-cyclodextrin-modified zirconia monolith	87:140	a phosphated β-cyclodextrin-modified zirconia monolith	87:140	Enantiomer separations of basic chiral compounds by capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith.
24661867	1	1	theme	zirconia	182:189	arg1	phase					234:238	the chiral stationary phase	212:238	the chiral stationary phase	212:238	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	1	theme	zirconia	182:189	arg1	monolith					191:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	3	2	theme	15-mM	658:662	arg1	buffer					672:677	a 15-mM aqueous buffer	656:677	a 15-mM aqueous buffer of pH 5.0 with 5mM PCD	656:700	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	3	2	theme	15-mM	658:662	arg1	conditions					584:593	Optimum mobile phase conditions	563:593	Optimum mobile phase conditions for CEC separation of the compounds studied	563:637	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	3	3	theme	compounds	621:629	arg1	separation					603:612	CEC separation	599:612	CEC separation of the compounds studied	599:637	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	3	4	with	buffer	672:677	arg1	PCD					698:700	5mM PCD	694:700	5mM PCD	694:700	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	0	5	theme	zirconia	124:131	arg1	monolith					133:140	a phosphated β-cyclodextrin-modified zirconia monolith	87:140	a phosphated β-cyclodextrin-modified zirconia monolith	87:140	Enantiomer separations of basic chiral compounds by capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith.
24661867	3	6	theme	aqueous	664:670	arg1	buffer					672:677	a 15-mM aqueous buffer	656:677	a 15-mM aqueous buffer of pH 5.0 with 5mM PCD	656:700	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	3	6	theme	aqueous	664:670	arg1	conditions					584:593	Optimum mobile phase conditions	563:593	Optimum mobile phase conditions for CEC separation of the compounds studied	563:637	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	4	7	theme	mobile	822:827	arg1	phase					829:833	the optimal mobile phase	810:833	the optimal mobile phase	810:833	Baseline separations of enantiomers of CIT, MET and SER, and partial separation for ATE were achieved with the optimal mobile phase.
24661867	4	8	theme	separation	772:781	arg1	enantiomers					727:737	enantiomers	727:737	enantiomers of CIT, MET and SER, and partial separation	727:781	Baseline separations of enantiomers of CIT, MET and SER, and partial separation for ATE were achieved with the optimal mobile phase.
24661867	4	9	theme	enantiomers	727:737	arg1	separations					712:722	Baseline separations	703:722	Baseline separations of enantiomers of CIT, MET and SER, and partial separation for ATE	703:789	Baseline separations of enantiomers of CIT, MET and SER, and partial separation for ATE were achieved with the optimal mobile phase.
24661867	1	10	dep	atenolol	394:401	arg1	ATE					404:406	ATE	404:406	ATE	404:406	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	0	11	theme	β-cyclodextrin-modified	100:122	arg1	monolith					133:140	a phosphated β-cyclodextrin-modified zirconia monolith	87:140	a phosphated β-cyclodextrin-modified zirconia monolith	87:140	Enantiomer separations of basic chiral compounds by capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith.
24661867	4	12	theme	optimal	814:820	arg1	phase					829:833	the optimal mobile phase	810:833	the optimal mobile phase	810:833	Baseline separations of enantiomers of CIT, MET and SER, and partial separation for ATE were achieved with the optimal mobile phase.
24661867	3	13	theme	5mM	694:696	arg1	PCD					698:700	5mM PCD	694:700	5mM PCD	694:700	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	4	14	theme	CIT	742:744	arg1	enantiomers					727:737	enantiomers	727:737	enantiomers of CIT, MET and SER, and partial separation	727:781	Baseline separations of enantiomers of CIT, MET and SER, and partial separation for ATE were achieved with the optimal mobile phase.
24661867	2	15	theme	mobile	492:497	arg1	phases					499:504	reversed-phase mobile phases	477:504	reversed-phase mobile phases of varying pH, buffer and organic modifier compositions	477:560	Migration, chiral selectivity and resolution data were measured in reversed-phase mobile phases of varying pH, buffer and organic modifier compositions.
24661867	3	16	theme	pH	682:683	arg1	buffer					672:677	a 15-mM aqueous buffer	656:677	a 15-mM aqueous buffer of pH 5.0 with 5mM PCD	656:700	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	3	16	theme	pH	682:683	arg1	conditions					584:593	Optimum mobile phase conditions	563:593	Optimum mobile phase conditions for CEC separation of the compounds studied	563:637	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	2	17	theme	chiral	421:426	arg1	selectivity					428:438	chiral selectivity	421:438	chiral selectivity	421:438	Migration, chiral selectivity and resolution data were measured in reversed-phase mobile phases of varying pH, buffer and organic modifier compositions.
24661867	0	18	theme	Enantiomer	0:9	arg1	separations					11:21	Enantiomer separations	0:21	Enantiomer separations of basic chiral compounds by capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith.	0:141	Enantiomer separations of basic chiral compounds by capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith.
24661867	0	19	theme	basic	26:30	arg1	compounds					39:47	basic chiral compounds	26:47	basic chiral compounds	26:47	Enantiomer separations of basic chiral compounds by capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith.
24661867	1	20	theme	basic	304:308	arg1	sertraline					355:364	sertraline	355:364	sertraline (SER)	355:370	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	20	theme	basic	304:308	arg1	metoprolol					337:346	metoprolol	337:346	metoprolol (MET)	337:352	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	20	theme	basic	304:308	arg1	compounds					317:325	four basic chiral compounds	299:325	four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE)	299:407	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	20	theme	basic	304:308	arg1	atenolol					394:401	atenolol	394:401	atenolol (ATE)	394:407	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	20	theme	basic	304:308	arg1	citalopram					373:382	citalopram	373:382	citalopram (CIT)	373:388	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	4	21	theme	partial	764:770	arg1	separation					772:781	partial separation	764:781	partial separation	764:781	Baseline separations of enantiomers of CIT, MET and SER, and partial separation for ATE were achieved with the optimal mobile phase.
24661867	1	22	theme	chiral	216:221	arg1	phase					234:238	the chiral stationary phase	212:238	the chiral stationary phase	212:238	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	22	theme	chiral	216:221	arg1	monolith					191:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	23	theme	chiral	310:315	arg1	sertraline					355:364	sertraline	355:364	sertraline (SER)	355:370	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	23	theme	chiral	310:315	arg1	metoprolol					337:346	metoprolol	337:346	metoprolol (MET)	337:352	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	23	theme	chiral	310:315	arg1	compounds					317:325	four basic chiral compounds	299:325	four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE)	299:407	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	23	theme	chiral	310:315	arg1	atenolol					394:401	atenolol	394:401	atenolol (ATE)	394:407	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	23	theme	chiral	310:315	arg1	citalopram					373:382	citalopram	373:382	citalopram (CIT)	373:388	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	2	24	theme	buffer	521:526	arg1	phases					499:504	reversed-phase mobile phases	477:504	reversed-phase mobile phases of varying pH, buffer and organic modifier compositions	477:560	Migration, chiral selectivity and resolution data were measured in reversed-phase mobile phases of varying pH, buffer and organic modifier compositions.
24661867	4	25	theme	Baseline	703:710	arg1	separations					712:722	Baseline separations	703:722	Baseline separations of enantiomers of CIT, MET and SER, and partial separation for ATE	703:789	Baseline separations of enantiomers of CIT, MET and SER, and partial separation for ATE were achieved with the optimal mobile phase.
24661867	1	26	theme	stationary	223:232	arg1	phase					234:238	the chiral stationary phase	212:238	the chiral stationary phase	212:238	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	26	theme	stationary	223:232	arg1	monolith					191:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	0	27	theme	compounds	39:47	arg1	separations					11:21	Enantiomer separations	0:21	Enantiomer separations of basic chiral compounds by capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith.	0:141	Enantiomer separations of basic chiral compounds by capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith.
24661867	1	28	theme	compounds	317:325	arg1	separation					285:294	separation	285:294	separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE)	285:407	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	3	29	theme	phase	578:582	arg1	buffer					672:677	a 15-mM aqueous buffer	656:677	a 15-mM aqueous buffer of pH 5.0 with 5mM PCD	656:700	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	3	29	theme	phase	578:582	arg1	conditions					584:593	Optimum mobile phase conditions	563:593	Optimum mobile phase conditions for CEC separation of the compounds studied	563:637	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	2	30	theme	resolution	444:453	arg1	data					455:458	resolution data	444:458	resolution data	444:458	Migration, chiral selectivity and resolution data were measured in reversed-phase mobile phases of varying pH, buffer and organic modifier compositions.
24661867	0	31	theme	chiral	32:37	arg1	compounds					39:47	basic chiral compounds	26:47	basic chiral compounds	26:47	Enantiomer separations of basic chiral compounds by capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith.
24661867	0	32	theme	capillary	52:60	arg1	electrochromatography					62:82	capillary electrochromatography	52:82	capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith	52:140	Enantiomer separations of basic chiral compounds by capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith.
24661867	2	33	theme	varying	509:515	arg1	pH					517:518	varying pH	509:518	varying pH	509:518	Migration, chiral selectivity and resolution data were measured in reversed-phase mobile phases of varying pH, buffer and organic modifier compositions.
24661867	0	34	from	electrochromatography	62:82	arg1	monolith					133:140	a phosphated β-cyclodextrin-modified zirconia monolith	87:140	a phosphated β-cyclodextrin-modified zirconia monolith	87:140	Enantiomer separations of basic chiral compounds by capillary electrochromatography on a phosphated β-cyclodextrin-modified zirconia monolith.
24661867	3	35	theme	Optimum	563:569	arg1	buffer					672:677	a 15-mM aqueous buffer	656:677	a 15-mM aqueous buffer of pH 5.0 with 5mM PCD	656:700	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	3	35	theme	Optimum	563:569	arg1	conditions					584:593	Optimum mobile phase conditions	563:593	Optimum mobile phase conditions for CEC separation of the compounds studied	563:637	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	1	36	theme	Phosphated	143:152	arg1	phase					234:238	the chiral stationary phase	212:238	the chiral stationary phase	212:238	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	36	theme	Phosphated	143:152	arg1	monolith					191:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	2	37	theme	pH	517:518	arg1	phases					499:504	reversed-phase mobile phases	477:504	reversed-phase mobile phases of varying pH, buffer and organic modifier compositions	477:560	Migration, chiral selectivity and resolution data were measured in reversed-phase mobile phases of varying pH, buffer and organic modifier compositions.
24661867	2	38	theme	compositions	549:560	arg1	phases					499:504	reversed-phase mobile phases	477:504	reversed-phase mobile phases of varying pH, buffer and organic modifier compositions	477:560	Migration, chiral selectivity and resolution data were measured in reversed-phase mobile phases of varying pH, buffer and organic modifier compositions.
24661867	3	39	theme	mobile	571:576	arg1	buffer					672:677	a 15-mM aqueous buffer	656:677	a 15-mM aqueous buffer of pH 5.0 with 5mM PCD	656:700	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	3	39	theme	mobile	571:576	arg1	conditions					584:593	Optimum mobile phase conditions	563:593	Optimum mobile phase conditions for CEC separation of the compounds studied	563:637	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	2	40	theme	modifier	540:547	arg1	compositions					549:560	organic modifier compositions	532:560	organic modifier compositions	532:560	Migration, chiral selectivity and resolution data were measured in reversed-phase mobile phases of varying pH, buffer and organic modifier compositions.
24661867	4	41	theme	MET	747:749	arg1	enantiomers					727:737	enantiomers	727:737	enantiomers of CIT, MET and SER, and partial separation	727:781	Baseline separations of enantiomers of CIT, MET and SER, and partial separation for ATE were achieved with the optimal mobile phase.
24661867	1	42	theme	capillary	243:251	arg1	CEC					276:278	CEC	276:278	CEC	276:278	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	42	theme	capillary	243:251	arg1	electrochromatography					253:273	capillary electrochromatography	243:273	capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE)	243:407	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	2	43	theme	organic	532:538	arg1	compositions					549:560	organic modifier compositions	532:560	organic modifier compositions	532:560	Migration, chiral selectivity and resolution data were measured in reversed-phase mobile phases of varying pH, buffer and organic modifier compositions.
24661867	2	44	theme	reversed-phase	477:490	arg1	phases					499:504	reversed-phase mobile phases	477:504	reversed-phase mobile phases of varying pH, buffer and organic modifier compositions	477:560	Migration, chiral selectivity and resolution data were measured in reversed-phase mobile phases of varying pH, buffer and organic modifier compositions.
24661867	1	45	used	used	204:207	arg2	monolith					191:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	45	used	used	204:207	arg2	phase					234:238	the chiral stationary phase	212:238	the chiral stationary phase	212:238	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	3	46	theme	CEC	599:601	arg1	separation					603:612	CEC separation	599:612	CEC separation of the compounds studied	599:637	Optimum mobile phase conditions for CEC separation of the compounds studied were found to be a 15-mM aqueous buffer of pH 5.0 with 5mM PCD.
24661867	1	47	theme	-coated	174:180	arg1	phase					234:238	the chiral stationary phase	212:238	the chiral stationary phase	212:238	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
24661867	1	47	theme	-coated	174:180	arg1	monolith					191:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith	143:198	Phosphated β-cyclodextrin (PCD)-coated zirconia monolith was used as the chiral stationary phase in capillary electrochromatography (CEC) for separation of four basic chiral compounds including metoprolol (MET), sertraline (SER), citalopram (CIT) and atenolol (ATE).
27086715	0	0	theme	antibacterial	81:93	arg1	activity					95:102	the antibacterial activity	77:102	the antibacterial activity of polymeric nanoparticles	77:129	A microscopy method for scanning transmission electron microscopy imaging of the antibacterial activity of polymeric nanoparticles on a biofilm with an ionic liquid.
27086715	2	1	theme	method	488:493	arg1	combination					454:464	The combination	450:464	The combination of sample preparation method using a hydrophilic ionic liquid (IL) and STEM observation using the cooling holder	450:577	The combination of sample preparation method using a hydrophilic ionic liquid (IL) and STEM observation using the cooling holder eliminates the need for specialized equipment and techniques for biological sample preparation.
27086715	7	2	from	level	1553:1557	arg1	visualization					1482:1494	the visualization	1478:1494	the visualization of other deformable composite materials at the nanoscale level	1478:1557	In addition, this technique could contribute to the visualization of other deformable composite materials at the nanoscale level.
27086715	6	3	theme	target	1412:1417	arg1	materials					1419:1427	target materials	1412:1427	target materials	1412:1427	The developed simple sample preparation method would be helpful to understand the interaction of drugs with target materials.
27086715	7	4	theme	composite	1516:1524	arg1	materials					1526:1534	other deformable composite materials	1499:1534	other deformable composite materials	1499:1534	In addition, this technique could contribute to the visualization of other deformable composite materials at the nanoscale level.
27086715	2	5	theme	preparation	476:486	arg1	method					488:493	sample preparation method	469:493	sample preparation method using a hydrophilic ionic liquid (IL) and STEM observation using the cooling holder	469:577	The combination of sample preparation method using a hydrophilic ionic liquid (IL) and STEM observation using the cooling holder eliminates the need for specialized equipment and techniques for biological sample preparation.
27086715	3	6	theme	dark-field	687:696	arg1	results					703:709	The annular dark-field STEM results	675:709	The annular dark-field STEM results	675:709	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	2	7	theme	sample	655:660	arg1	preparation					662:672	biological sample preparation	644:672	biological sample preparation	644:672	The combination of sample preparation method using a hydrophilic ionic liquid (IL) and STEM observation using the cooling holder eliminates the need for specialized equipment and techniques for biological sample preparation.
27086715	9	8	theme	Part	1610:1613	arg1	B					1615:1615	Part B	1610:1615	J Biomed Mater Res Part B: Appl Biomater, 105B: 1432-1437, 2017.	1591:1654	J Biomed Mater Res Part B: Appl Biomater, 105B: 1432-1437, 2017.
27086715	5	9	theme	PLGA	1239:1242	arg1	NPs					1244:1246	PLGA NPs	1239:1246	PLGA NPs	1239:1246	The optimized method allows microstructure of the biofilm treated with PLGA NPs to be maintained for 25 min at a current flow of 40 pA.
27086715	5	10	theme	optimized	1172:1180	arg1	method					1182:1187	The optimized method	1168:1187	The optimized method	1168:1187	The optimized method allows microstructure of the biofilm treated with PLGA NPs to be maintained for 25 min at a current flow of 40 pA.
27086715	6	11	theme	developed	1308:1316	arg1	helpful					1360:1366	helpful	1360:1366	helpful	1360:1366	The developed simple sample preparation method would be helpful to understand the interaction of drugs with target materials.
27086715	6	11	theme	developed	1308:1316	arg1	method					1344:1349	The developed simple sample preparation method	1304:1349	The developed simple sample preparation method	1304:1349	The developed simple sample preparation method would be helpful to understand the interaction of drugs with target materials.
27086715	3	12	theme	STEM	698:701	arg1	results					703:709	The annular dark-field STEM results	675:709	The annular dark-field STEM results	675:709	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	1	13	theme	biofilm	430:436	arg1	infections					438:447	biofilm infections	430:447	biofilm infections	430:447	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	6	14	theme	drugs	1401:1405	arg1	interaction					1386:1396	the interaction	1382:1396	the interaction of drugs with target materials	1382:1427	The developed simple sample preparation method would be helpful to understand the interaction of drugs with target materials.
27086715	7	15	theme	other	1499:1503	arg1	materials					1526:1534	other deformable composite materials	1499:1534	other deformable composite materials	1499:1534	In addition, this technique could contribute to the visualization of other deformable composite materials at the nanoscale level.
27086715	5	16	theme	biofilm	1218:1224	arg1	microstructure					1196:1209	microstructure	1196:1209	microstructure of the biofilm treated with PLGA NPs to be maintained for 25 min at a current flow of 40 pA	1196:1301	The optimized method allows microstructure of the biofilm treated with PLGA NPs to be maintained for 25 min at a current flow of 40 pA.
27086715	5	17	theme	current	1281:1287	arg1	flow					1289:1292	a current flow	1279:1292	a current flow of 40 pA	1279:1301	The optimized method allows microstructure of the biofilm treated with PLGA NPs to be maintained for 25 min at a current flow of 40 pA.
27086715	7	18	theme	deformable	1505:1514	arg1	materials					1526:1534	other deformable composite materials	1499:1534	other deformable composite materials	1499:1534	In addition, this technique could contribute to the visualization of other deformable composite materials at the nanoscale level.
27086715	0	19	theme	nanoparticles	117:129	arg1	activity					95:102	the antibacterial activity	77:102	the antibacterial activity of polymeric nanoparticles	77:129	A microscopy method for scanning transmission electron microscopy imaging of the antibacterial activity of polymeric nanoparticles on a biofilm with an ionic liquid.
27086715	6	20	with	interaction	1386:1396	arg1	materials					1419:1427	target materials	1412:1427	target materials	1412:1427	The developed simple sample preparation method would be helpful to understand the interaction of drugs with target materials.
27086715	6	21	theme	sample	1325:1330	arg1	helpful					1360:1366	helpful	1360:1366	helpful	1360:1366	The developed simple sample preparation method would be helpful to understand the interaction of drugs with target materials.
27086715	6	21	theme	sample	1325:1330	arg1	method					1344:1349	The developed simple sample preparation method	1304:1349	The developed simple sample preparation method	1304:1349	The developed simple sample preparation method would be helpful to understand the interaction of drugs with target materials.
27086715	0	22	theme	polymeric	107:115	arg1	nanoparticles					117:129	polymeric nanoparticles	107:129	polymeric nanoparticles	107:129	A microscopy method for scanning transmission electron microscopy imaging of the antibacterial activity of polymeric nanoparticles on a biofilm with an ionic liquid.
27086715	8	23	dep	©	1560:1560	arg1	Inc.					1586:1589	Inc.	1586:1589	Inc.	1586:1589	© 2016 Wiley Periodicals, Inc.
27086715	0	24	theme	transmission	33:44	arg1	microscopy					55:64	scanning transmission electron microscopy	24:64	scanning transmission electron microscopy	24:64	A microscopy method for scanning transmission electron microscopy imaging of the antibacterial activity of polymeric nanoparticles on a biofilm with an ionic liquid.
27086715	3	25	theme	-loaded + CS-modified	858:878	arg1	PLGA					880:883	-loaded + CS-modified PLGA	858:883	-loaded + CS-modified PLGA	858:883	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	0	26	theme	microscopy	2:11	arg1	method					13:18	A microscopy method	0:18	A microscopy method for scanning transmission electron microscopy imaging of the antibacterial activity of polymeric nanoparticles on a biofilm with an ionic liquid.	0:164	A microscopy method for scanning transmission electron microscopy imaging of the antibacterial activity of polymeric nanoparticles on a biofilm with an ionic liquid.
27086715	1	27	theme	Staphylococcus	358:371	arg1	cells					395:399	Staphylococcus epidermidis bacterial cells	358:399	Staphylococcus epidermidis bacterial cells	358:399	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	2	28	theme	specialized	603:613	arg1	equipment					615:623	specialized equipment	603:623	specialized equipment	603:623	The combination of sample preparation method using a hydrophilic ionic liquid (IL) and STEM observation using the cooling holder eliminates the need for specialized equipment and techniques for biological sample preparation.
27086715	3	29	theme	poly-	757:761	arg1	NPs					795:797	biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs	743:797	biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs	743:797	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	3	30	theme	solvent	907:913	arg1	diffusion					915:923	emulsion solvent diffusion	898:923	emulsion solvent diffusion	898:923	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	3	31	dep	NPs	795:797	arg1	PLGA					800:803	PLGA	800:803	PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA	800:883	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	2	32	theme	STEM	537:540	arg1	observation					542:552	a hydrophilic ionic liquid (IL) and STEM observation	501:552	observation	542:552	The combination of sample preparation method using a hydrophilic ionic liquid (IL) and STEM observation using the cooling holder eliminates the need for specialized equipment and techniques for biological sample preparation.
27086715	6	33	theme	simple	1318:1323	arg1	helpful					1360:1366	helpful	1360:1366	helpful	1360:1366	The developed simple sample preparation method would be helpful to understand the interaction of drugs with target materials.
27086715	6	33	theme	simple	1318:1323	arg1	method					1344:1349	The developed simple sample preparation method	1304:1349	The developed simple sample preparation method	1304:1349	The developed simple sample preparation method would be helpful to understand the interaction of drugs with target materials.
27086715	4	34	theme	STEM	1023:1026	arg1	observation					1028:1038	STEM observation	1023:1038	STEM observation	1023:1038	To confirm damage to the sample during STEM observation, we observed the PLGA NPs and the biofilm treated with PLGA NPs by both the conventional method and the newly developed method.
27086715	1	35	theme	antibacterial	268:280	arg1	activity					282:289	the antibacterial activity	264:289	the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells	264:399	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	4	36	theme	PLGA	1095:1098	arg1	NPs					1100:1102	PLGA NPs	1095:1102	PLGA NPs	1095:1102	To confirm damage to the sample during STEM observation, we observed the PLGA NPs and the biofilm treated with PLGA NPs by both the conventional method and the newly developed method.
27086715	2	37	theme	sample	469:474	arg1	method					488:493	sample preparation method	469:493	sample preparation method using a hydrophilic ionic liquid (IL) and STEM observation using the cooling holder	469:577	The combination of sample preparation method using a hydrophilic ionic liquid (IL) and STEM observation using the cooling holder eliminates the need for specialized equipment and techniques for biological sample preparation.
27086715	3	38	theme	biodegradable	743:755	arg1	poly-					757:761	biodegradable poly-	743:761	biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs	743:797	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	3	38	theme	biodegradable	743:755	arg1	DL-lactide-co-glycolide					763:785	DL-lactide-co-glycolide	763:785	DL-lactide-co-glycolide	763:785	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	3	38	theme	biodegradable	743:755	arg1	PLGA					789:792	PLGA	789:792	PLGA	789:792	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	1	39	theme	bacterial	385:393	arg1	cells					395:399	Staphylococcus epidermidis bacterial cells	358:399	Staphylococcus epidermidis bacterial cells	358:399	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	3	40	theme	emulsion	898:905	arg1	diffusion					915:923	emulsion solvent diffusion	898:923	emulsion solvent diffusion	898:923	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	0	41	theme	scanning	24:31	arg1	microscopy					55:64	scanning transmission electron microscopy	24:64	scanning transmission electron microscopy	24:64	A microscopy method for scanning transmission electron microscopy imaging of the antibacterial activity of polymeric nanoparticles on a biofilm with an ionic liquid.
27086715	7	42	theme	materials	1526:1534	arg1	visualization					1482:1494	the visualization	1478:1494	the visualization of other deformable composite materials at the nanoscale level	1478:1557	In addition, this technique could contribute to the visualization of other deformable composite materials at the nanoscale level.
27086715	3	43	theme	antibacterial	945:957	arg1	activities					959:968	different antibacterial activities	935:968	different antibacterial activities	935:968	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	1	44	theme	scanning	196:203	arg1	microscopy					227:236	scanning transmission electron microscopy	196:236	a scanning transmission electron microscopy (STEM) method	194:250	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	1	44	theme	scanning	196:203	arg1	STEM					239:242	STEM	239:242	STEM	239:242	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	1	45	dep	Staphylococcus	358:371	arg1	epidermidis					373:383	epidermidis	373:383	epidermidis	373:383	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	2	46	theme	cooling	564:570	arg1	holder					572:577	the cooling holder	560:577	the cooling holder	560:577	The combination of sample preparation method using a hydrophilic ionic liquid (IL) and STEM observation using the cooling holder eliminates the need for specialized equipment and techniques for biological sample preparation.
27086715	0	47	theme	electron	46:53	arg1	microscopy					55:64	scanning transmission electron microscopy	24:64	scanning transmission electron microscopy	24:64	A microscopy method for scanning transmission electron microscopy imaging of the antibacterial activity of polymeric nanoparticles on a biofilm with an ionic liquid.
27086715	1	48	theme	polymeric	302:310	arg1	NPs					327:329	NPs	327:329	NPs	327:329	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	1	48	theme	polymeric	302:310	arg1	nanoparticles					312:324	organic polymeric nanoparticles	294:324	organic polymeric nanoparticles (NPs)	294:330	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	9	49	dep	Res	1606:1608	arg1	1432-1437					1639:1647	1432-1437	1639:1647	1432-1437	1639:1647	J Biomed Mater Res Part B: Appl Biomater, 105B: 1432-1437, 2017.
27086715	9	49	dep	Res	1606:1608	arg1	105B					1633:1636	105B	1633:1636	105B	1633:1636	J Biomed Mater Res Part B: Appl Biomater, 105B: 1432-1437, 2017.
27086715	9	49	dep	Res	1606:1608	arg1	B					1615:1615	Part B	1610:1615	J Biomed Mater Res Part B: Appl Biomater, 105B: 1432-1437, 2017.	1591:1654	J Biomed Mater Res Part B: Appl Biomater, 105B: 1432-1437, 2017.
27086715	9	49	dep	Res	1606:1608	arg1	Biomater					1623:1630	Biomater	1623:1630	Biomater	1623:1630	J Biomed Mater Res Part B: Appl Biomater, 105B: 1432-1437, 2017.
27086715	1	50	theme	nanoparticles	312:324	arg1	activity					282:289	the antibacterial activity	264:289	the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells	264:399	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	3	51	theme	annular	679:685	arg1	results					703:709	The annular dark-field STEM results	675:709	The annular dark-field STEM results	675:709	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	2	52	theme	biological	644:653	arg1	preparation					662:672	biological sample preparation	644:672	biological sample preparation	644:672	The combination of sample preparation method using a hydrophilic ionic liquid (IL) and STEM observation using the cooling holder eliminates the need for specialized equipment and techniques for biological sample preparation.
27086715	0	53	with	biofilm	136:142	arg1	liquid					158:163	liquid	158:163	liquid	158:163	A microscopy method for scanning transmission electron microscopy imaging of the antibacterial activity of polymeric nanoparticles on a biofilm with an ionic liquid.
27086715	1	54	theme	transmission	205:216	arg1	microscopy					227:236	scanning transmission electron microscopy	196:236	a scanning transmission electron microscopy (STEM) method	194:250	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	1	54	theme	transmission	205:216	arg1	STEM					239:242	STEM	239:242	STEM	239:242	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	3	55	theme	different	935:943	arg1	activities					959:968	different antibacterial activities	935:968	different antibacterial activities	935:968	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	3	56	dep	chitosan	819:826	arg1	PLGA					880:883	-loaded + CS-modified PLGA	858:883	-loaded + CS-modified PLGA	858:883	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	4	57	theme	developed	1150:1158	arg1	method					1160:1165	the newly developed method	1140:1165	the newly developed method	1140:1165	To confirm damage to the sample during STEM observation, we observed the PLGA NPs and the biofilm treated with PLGA NPs by both the conventional method and the newly developed method.
27086715	5	58	theme	pA	1300:1301	arg1	flow					1289:1292	a current flow	1279:1292	a current flow of 40 pA	1279:1301	The optimized method allows microstructure of the biofilm treated with PLGA NPs to be maintained for 25 min at a current flow of 40 pA.
27086715	1	59	theme	electron	218:225	arg1	microscopy					227:236	scanning transmission electron microscopy	196:236	a scanning transmission electron microscopy (STEM) method	194:250	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	1	59	theme	electron	218:225	arg1	STEM					239:242	STEM	239:242	STEM	239:242	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	4	60	theme	conventional	1116:1127	arg1	method					1129:1134	the conventional method	1112:1134	the conventional method	1112:1134	To confirm damage to the sample during STEM observation, we observed the PLGA NPs and the biofilm treated with PLGA NPs by both the conventional method and the newly developed method.
27086715	4	61	theme	PLGA	1057:1060	arg1	NPs					1062:1064	the PLGA NPs	1053:1064	the PLGA NPs	1053:1064	To confirm damage to the sample during STEM observation, we observed the PLGA NPs and the biofilm treated with PLGA NPs by both the conventional method and the newly developed method.
27086715	1	62	theme	microscopy	227:236	arg1	method					245:250	a scanning transmission electron microscopy (STEM) method	194:250	a scanning transmission electron microscopy (STEM) method	194:250	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	6	63	theme	preparation	1332:1342	arg1	helpful					1360:1366	helpful	1360:1366	helpful	1360:1366	The developed simple sample preparation method would be helpful to understand the interaction of drugs with target materials.
27086715	6	63	theme	preparation	1332:1342	arg1	method					1344:1349	The developed simple sample preparation method	1304:1349	The developed simple sample preparation method	1304:1349	The developed simple sample preparation method would be helpful to understand the interaction of drugs with target materials.
27086715	3	64	theme	NPs	795:797	arg1	types					734:738	the two types	726:738	the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion	726:923	The annular dark-field STEM results indicated that the two types of biodegradable poly-(DL-lactide-co-glycolide) (PLGA) NPs: PLGA modified with chitosan (CS), and clarithromycin (CAM)-loaded + CS-modified PLGA, prepared by emulsion solvent diffusion exhibited different antibacterial activities in nanoscale.
27086715	7	65	theme	nanoscale	1543:1551	arg1	level					1553:1557	the nanoscale level	1539:1557	the nanoscale level	1539:1557	In addition, this technique could contribute to the visualization of other deformable composite materials at the nanoscale level.
27086715	1	66	theme	organic	294:300	arg1	NPs					327:329	NPs	327:329	NPs	327:329	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
27086715	1	66	theme	organic	294:300	arg1	nanoparticles					312:324	organic polymeric nanoparticles	294:324	organic polymeric nanoparticles (NPs)	294:330	In this study, we developed a scanning transmission electron microscopy (STEM) method for imaging the antibacterial activity of organic polymeric nanoparticles (NPs) toward biofilms formed by Staphylococcus epidermidis bacterial cells, for optimizing NPs to treat biofilm infections.
25839124	9	0	theme	adipose	1398:1404	arg1	engineering					1413:1423	adipose tissue engineering	1398:1423	adipose tissue engineering	1398:1423	A preliminary in vivo study demonstrated the usefulness of the hydrogel as an injectable scaffold for adipose tissue engineering.
25839124	9	1	dep	in	1310:1311	arg1	vivo					1313:1316	vivo	1313:1316	vivo	1313:1316	A preliminary in vivo study demonstrated the usefulness of the hydrogel as an injectable scaffold for adipose tissue engineering.
25839124	7	2	theme	human	1124:1128	arg1	cells					1151:1155	human adipose-derived stem cells	1124:1155	human adipose-derived stem cells (ASCs)	1124:1162	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	7	2	theme	human	1124:1128	arg1	ASCs					1158:1161	ASCs	1158:1161	ASCs	1158:1161	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	6	3	theme	compressive	949:959	arg1	modulus					961:967	compressive modulus	949:967	compressive modulus	949:967	The in vitro gelation, morphologies, equilibrium swelling, compressive modulus and degradation of the composite hydrogels were examined.
25839124	10	4	theme	click	1502:1506	arg1	chemistry					1508:1516	the metal-free click chemistry	1487:1516	the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications	1487:1599	These characteristics provide a potential opportunity to use the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications.
25839124	0	5	theme	tissue	103:108	arg1	engineering					110:120	soft tissue engineering	98:120	soft tissue engineering	98:120	Cytocompatible in situ forming chitosan/hyaluronan hydrogels via a metal-free click chemistry for soft tissue engineering.
25839124	10	6	theme	metal-free	1491:1500	arg1	chemistry					1508:1516	the metal-free click chemistry	1487:1516	the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications	1487:1599	These characteristics provide a potential opportunity to use the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications.
25839124	4	7	theme	derivatives	700:710	arg1	groups					675:680	OB and azido groups	662:680	OB and azido groups of polysaccharide derivatives	662:710	The gelation is attributed to the triazole ring formation between OB and azido groups of polysaccharide derivatives.
25839124	4	7	theme	derivatives	700:710	arg1	derivatives					700:710	polysaccharide derivatives	685:710	polysaccharide derivatives	685:710	The gelation is attributed to the triazole ring formation between OB and azido groups of polysaccharide derivatives.
25839124	4	8	theme	triazole	630:637	arg1	formation					644:652	the triazole ring formation	626:652	the triazole ring formation between OB and azido groups of polysaccharide derivatives	626:710	The gelation is attributed to the triazole ring formation between OB and azido groups of polysaccharide derivatives.
25839124	0	9	theme	soft	98:101	arg1	engineering					110:120	soft tissue engineering	98:120	soft tissue engineering	98:120	Cytocompatible in situ forming chitosan/hyaluronan hydrogels via a metal-free click chemistry for soft tissue engineering.
25839124	7	10	link	adipose-derived	1130:1144	arg1	cells					1151:1155	human adipose-derived stem cells	1124:1155	human adipose-derived stem cells (ASCs)	1124:1162	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	7	10	link	adipose-derived	1130:1144	arg1	ASCs					1158:1161	ASCs	1158:1161	ASCs	1158:1161	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	2	11	theme	hydrogels	313:321	arg1	class					257:261	a new class	251:261	a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry	251:391	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	9	12	theme	tissue	1406:1411	arg1	engineering					1413:1423	adipose tissue engineering	1398:1423	adipose tissue engineering	1398:1423	A preliminary in vivo study demonstrated the usefulness of the hydrogel as an injectable scaffold for adipose tissue engineering.
25839124	1	13	theme	Injectable	123:132	arg1	materials					175:183	important cell scaffolding materials	148:183	important cell scaffolding materials for tissue engineering and regenerative medicine	148:232	Injectable hydrogels are important cell scaffolding materials for tissue engineering and regenerative medicine.
25839124	1	13	theme	Injectable	123:132	arg1	hydrogels					134:142	Injectable hydrogels	123:142	Injectable hydrogels	123:142	Injectable hydrogels are important cell scaffolding materials for tissue engineering and regenerative medicine.
25839124	7	14	theme	cells	1151:1155	arg1	encapsulation					1107:1119	encapsulation	1107:1119	encapsulation of human adipose-derived stem cells (ASCs)	1107:1162	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	2	15	theme	polysaccharide	298:311	arg1	hydrogels					313:321	biocompatible and biodegradable polysaccharide hydrogels	266:321	biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry	266:391	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	1	16	theme	tissue	189:194	arg1	engineering					196:206	tissue engineering	189:206	tissue engineering	189:206	Injectable hydrogels are important cell scaffolding materials for tissue engineering and regenerative medicine.
25839124	9	17	theme	hydrogel	1359:1366	arg1	usefulness					1341:1350	the usefulness	1337:1350	the usefulness of the hydrogel as an injectable scaffold for adipose tissue engineering	1337:1423	A preliminary in vivo study demonstrated the usefulness of the hydrogel as an injectable scaffold for adipose tissue engineering.
25839124	10	18	theme	hydrogels	1550:1558	arg1	preparation					1521:1531	preparation	1521:1531	preparation of biocompatible hydrogels for soft tissue engineering applications	1521:1599	These characteristics provide a potential opportunity to use the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications.
25839124	2	19	theme	biodegradable	284:296	arg1	hydrogels					313:321	biocompatible and biodegradable polysaccharide hydrogels	266:321	biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry	266:391	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	2	20	theme	copper	418:423	arg1	catalyst					425:432	copper catalyst	418:432	copper catalyst	418:432	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	7	21	theme	adipose-derived	1130:1144	arg1	cells					1151:1155	human adipose-derived stem cells	1124:1155	human adipose-derived stem cells (ASCs)	1124:1162	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	7	21	theme	adipose-derived	1130:1144	arg1	ASCs					1158:1161	ASCs	1158:1161	ASCs	1158:1161	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	5	22	theme	substitution	805:816	arg1	degrees					818:824	substitution degrees	805:824	substitution degrees of 58% and 47%	805:839	The molecular structures were verified by FT-IR spectroscopy and elemental analysis, giving substitution degrees of 58% and 47% for chitosan-OB and hyaluronan-AA, respectively.
25839124	6	23	theme	equilibrium	927:937	arg1	swelling					939:946	equilibrium swelling	927:946	equilibrium swelling	927:946	The in vitro gelation, morphologies, equilibrium swelling, compressive modulus and degradation of the composite hydrogels were examined.
25839124	3	24	theme	click	454:458	arg1	reaction					460:467	the metal-free click reaction	439:467	the metal-free click reaction	439:467	For the metal-free click reaction, chitosan and hyaluronan were modified with oxanorbornadiene (OB) and 11-azido-3,6,9-trioxaundecan-1-amine (AA), respectively.
25839124	7	25	theme	stem	1146:1149	arg1	cells					1151:1155	human adipose-derived stem cells	1124:1155	human adipose-derived stem cells (ASCs)	1124:1162	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	7	25	theme	stem	1146:1149	arg1	ASCs					1158:1161	ASCs	1158:1161	ASCs	1158:1161	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	10	26	theme	biocompatible	1536:1548	arg1	hydrogels					1550:1558	biocompatible hydrogels	1536:1558	biocompatible hydrogels	1536:1558	These characteristics provide a potential opportunity to use the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications.
25839124	5	27	theme	elemental	778:786	arg1	analysis					788:795	elemental analysis	778:795	elemental analysis	778:795	The molecular structures were verified by FT-IR spectroscopy and elemental analysis, giving substitution degrees of 58% and 47% for chitosan-OB and hyaluronan-AA, respectively.
25839124	1	28	theme	regenerative	212:223	arg1	medicine					225:232	regenerative medicine	212:232	regenerative medicine	212:232	Injectable hydrogels are important cell scaffolding materials for tissue engineering and regenerative medicine.
25839124	9	29	theme	injectable	1374:1383	arg1	scaffold					1385:1392	an injectable scaffold	1371:1392	an injectable scaffold for adipose tissue engineering	1371:1423	A preliminary in vivo study demonstrated the usefulness of the hydrogel as an injectable scaffold for adipose tissue engineering.
25839124	10	30	theme	tissue	1569:1574	arg1	applications					1588:1599	soft tissue engineering applications	1564:1599	soft tissue engineering applications	1564:1599	These characteristics provide a potential opportunity to use the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications.
25839124	10	31	theme	potential	1458:1466	arg1	opportunity					1468:1478	a potential opportunity	1456:1478	a potential opportunity to use the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications	1456:1599	These characteristics provide a potential opportunity to use the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications.
25839124	4	32	theme	azido	669:673	arg1	groups					675:680	OB and azido groups	662:680	OB and azido groups of polysaccharide derivatives	662:710	The gelation is attributed to the triazole ring formation between OB and azido groups of polysaccharide derivatives.
25839124	4	32	theme	azido	669:673	arg1	derivatives					700:710	polysaccharide derivatives	685:710	polysaccharide derivatives	685:710	The gelation is attributed to the triazole ring formation between OB and azido groups of polysaccharide derivatives.
25839124	8	33	theme	metal-free	1226:1235	arg1	hydrogel					1237:1244	this metal-free hydrogel	1221:1244	this metal-free hydrogel	1221:1244	Cell culture showed that this metal-free hydrogel could support survival and proliferation of ASCs.
25839124	10	34	from	chemistry	1508:1516	arg1	preparation					1521:1531	preparation	1521:1531	preparation of biocompatible hydrogels for soft tissue engineering applications	1521:1599	These characteristics provide a potential opportunity to use the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications.
25839124	5	35	theme	FT-IR	755:759	arg1	spectroscopy					761:772	FT-IR spectroscopy	755:772	FT-IR spectroscopy	755:772	The molecular structures were verified by FT-IR spectroscopy and elemental analysis, giving substitution degrees of 58% and 47% for chitosan-OB and hyaluronan-AA, respectively.
25839124	5	36	theme	%	831:831	arg1	degrees					818:824	substitution degrees	805:824	substitution degrees of 58% and 47%	805:839	The molecular structures were verified by FT-IR spectroscopy and elemental analysis, giving substitution degrees of 58% and 47% for chitosan-OB and hyaluronan-AA, respectively.
25839124	6	37	theme	hydrogels	1002:1010	arg1	modulus					961:967	compressive modulus	949:967	compressive modulus	949:967	The in vitro gelation, morphologies, equilibrium swelling, compressive modulus and degradation of the composite hydrogels were examined.
25839124	6	37	theme	hydrogels	1002:1010	arg1	degradation					973:983	degradation	973:983	degradation	973:983	The in vitro gelation, morphologies, equilibrium swelling, compressive modulus and degradation of the composite hydrogels were examined.
25839124	6	37	theme	hydrogels	1002:1010	arg1	swelling					939:946	equilibrium swelling	927:946	equilibrium swelling	927:946	The in vitro gelation, morphologies, equilibrium swelling, compressive modulus and degradation of the composite hydrogels were examined.
25839124	6	37	theme	hydrogels	1002:1010	arg1	gelation					903:910	The in vitro gelation	890:910	The in vitro gelation	890:910	The in vitro gelation, morphologies, equilibrium swelling, compressive modulus and degradation of the composite hydrogels were examined.
25839124	6	37	theme	hydrogels	1002:1010	arg1	morphologies					913:924	morphologies	913:924	morphologies	913:924	The in vitro gelation, morphologies, equilibrium swelling, compressive modulus and degradation of the composite hydrogels were examined.
25839124	2	38	theme	new	253:255	arg1	class					257:261	a new class	251:261	a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry	251:391	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	8	39	theme	ASCs	1290:1293	arg1	proliferation					1273:1285	proliferation	1273:1285	proliferation	1273:1285	Cell culture showed that this metal-free hydrogel could support survival and proliferation of ASCs.
25839124	8	39	theme	ASCs	1290:1293	arg1	survival					1260:1267	survival	1260:1267	survival	1260:1267	Cell culture showed that this metal-free hydrogel could support survival and proliferation of ASCs.
25839124	2	40	theme	catalyst	425:432	arg1	addition					406:413	the addition	402:413	the addition of copper catalyst	402:432	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	6	41	theme	composite	992:1000	arg1	hydrogels					1002:1010	the composite hydrogels	988:1010	the composite hydrogels	988:1010	The in vitro gelation, morphologies, equilibrium swelling, compressive modulus and degradation of the composite hydrogels were examined.
25839124	0	42	theme	chitosan/hyaluronan	31:49	arg1	hydrogels					51:59	chitosan/hyaluronan hydrogels	31:59	chitosan/hyaluronan hydrogels	31:59	Cytocompatible in situ forming chitosan/hyaluronan hydrogels via a metal-free click chemistry for soft tissue engineering.
25839124	7	43	theme	gel	1175:1177	arg1	matrix					1179:1184	the gel matrix	1171:1184	the gel matrix	1171:1184	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	2	44	attach	derived	323:329	arg1	chitosan					336:343	chitosan	336:343	chitosan	336:343	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	2	44	attach	derived	323:329	arg1	hyaluronan					349:358	hyaluronan	349:358	hyaluronan	349:358	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	2	44	attach	derived	323:329	arg2	hydrogels					313:321	biocompatible and biodegradable polysaccharide hydrogels	266:321	biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry	266:391	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	5	45	theme	molecular	717:725	arg1	structures					727:736	The molecular structures	713:736	The molecular structures	713:736	The molecular structures were verified by FT-IR spectroscopy and elemental analysis, giving substitution degrees of 58% and 47% for chitosan-OB and hyaluronan-AA, respectively.
25839124	6	46	theme	in	894:895	arg1	gelation					903:910	The in vitro gelation	890:910	The in vitro gelation	890:910	The in vitro gelation, morphologies, equilibrium swelling, compressive modulus and degradation of the composite hydrogels were examined.
25839124	2	47	theme	click	377:381	arg1	chemistry					383:391	a metal-free click chemistry	364:391	a metal-free click chemistry	364:391	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	5	48	theme	%	839:839	arg1	degrees					818:824	substitution degrees	805:824	substitution degrees of 58% and 47%	805:839	The molecular structures were verified by FT-IR spectroscopy and elemental analysis, giving substitution degrees of 58% and 47% for chitosan-OB and hyaluronan-AA, respectively.
25839124	7	49	theme	metal-free	1048:1057	arg1	hydrogel					1059:1066	the metal-free hydrogel	1044:1066	the metal-free hydrogel	1044:1066	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	2	50	theme	metal-free	366:375	arg1	chemistry					383:391	a metal-free click chemistry	364:391	a metal-free click chemistry	364:391	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	7	51	theme	cell	1073:1076	arg1	scaffold					1078:1085	a cell scaffold	1071:1085	a cell scaffold	1071:1085	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	9	52	theme	preliminary	1298:1308	arg1	study					1318:1322	A preliminary in vivo study	1296:1322	A preliminary in vivo study	1296:1322	A preliminary in vivo study demonstrated the usefulness of the hydrogel as an injectable scaffold for adipose tissue engineering.
25839124	7	53	theme	hydrogel	1059:1066	arg1	potential					1031:1039	The potential	1027:1039	The potential of the metal-free hydrogel as a cell scaffold	1027:1085	The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
25839124	3	54	mod	modified	499:506	arg3	AA					577:578	AA	577:578	AA	577:578	For the metal-free click reaction, chitosan and hyaluronan were modified with oxanorbornadiene (OB) and 11-azido-3,6,9-trioxaundecan-1-amine (AA), respectively.
25839124	3	54	mod	modified	499:506	arg3	oxanorbornadiene					513:528	oxanorbornadiene	513:528	oxanorbornadiene (OB)	513:533	For the metal-free click reaction, chitosan and hyaluronan were modified with oxanorbornadiene (OB) and 11-azido-3,6,9-trioxaundecan-1-amine (AA), respectively.
25839124	3	54	mod	modified	499:506	arg3	OB					531:532	OB	531:532	OB	531:532	For the metal-free click reaction, chitosan and hyaluronan were modified with oxanorbornadiene (OB) and 11-azido-3,6,9-trioxaundecan-1-amine (AA), respectively.
25839124	3	54	mod	modified	499:506	arg1	chitosan					470:477	chitosan	470:477	chitosan	470:477	For the metal-free click reaction, chitosan and hyaluronan were modified with oxanorbornadiene (OB) and 11-azido-3,6,9-trioxaundecan-1-amine (AA), respectively.
25839124	3	54	mod	modified	499:506	arg3	11-azido-3,6,9-trioxaundecan-1-amine					539:574	11-azido-3,6,9-trioxaundecan-1-amine	539:574	11-azido-3,6,9-trioxaundecan-1-amine (AA)	539:579	For the metal-free click reaction, chitosan and hyaluronan were modified with oxanorbornadiene (OB) and 11-azido-3,6,9-trioxaundecan-1-amine (AA), respectively.
25839124	3	54	mod	modified	499:506	arg1	hyaluronan					483:492	hyaluronan	483:492	hyaluronan	483:492	For the metal-free click reaction, chitosan and hyaluronan were modified with oxanorbornadiene (OB) and 11-azido-3,6,9-trioxaundecan-1-amine (AA), respectively.
25839124	10	55	theme	soft	1564:1567	arg1	applications					1588:1599	soft tissue engineering applications	1564:1599	soft tissue engineering applications	1564:1599	These characteristics provide a potential opportunity to use the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications.
25839124	8	56	theme	Cell	1196:1199	arg1	culture					1201:1207	Cell culture	1196:1207	Cell culture	1196:1207	Cell culture showed that this metal-free hydrogel could support survival and proliferation of ASCs.
25839124	10	57	theme	engineering	1576:1586	arg1	applications					1588:1599	soft tissue engineering applications	1564:1599	soft tissue engineering applications	1564:1599	These characteristics provide a potential opportunity to use the metal-free click chemistry in preparation of biocompatible hydrogels for soft tissue engineering applications.
25839124	9	58	theme	in	1310:1311	arg1	study					1318:1322	A preliminary in vivo study	1296:1322	A preliminary in vivo study	1296:1322	A preliminary in vivo study demonstrated the usefulness of the hydrogel as an injectable scaffold for adipose tissue engineering.
25839124	2	59	theme	biocompatible	266:278	arg1	hydrogels					313:321	biocompatible and biodegradable polysaccharide hydrogels	266:321	biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry	266:391	Here, we report a new class of biocompatible and biodegradable polysaccharide hydrogels derived from chitosan and hyaluronan via a metal-free click chemistry, without the addition of copper catalyst.
25839124	1	60	theme	important	148:156	arg1	materials					175:183	important cell scaffolding materials	148:183	important cell scaffolding materials for tissue engineering and regenerative medicine	148:232	Injectable hydrogels are important cell scaffolding materials for tissue engineering and regenerative medicine.
25839124	1	60	theme	important	148:156	arg1	hydrogels					134:142	Injectable hydrogels	123:142	Injectable hydrogels	123:142	Injectable hydrogels are important cell scaffolding materials for tissue engineering and regenerative medicine.
25839124	4	61	theme	ring	639:642	arg1	formation					644:652	the triazole ring formation	626:652	the triazole ring formation between OB and azido groups of polysaccharide derivatives	626:710	The gelation is attributed to the triazole ring formation between OB and azido groups of polysaccharide derivatives.
25839124	0	62	theme	click	78:82	arg1	chemistry					84:92	a metal-free click chemistry	65:92	a metal-free click chemistry for soft tissue engineering	65:120	Cytocompatible in situ forming chitosan/hyaluronan hydrogels via a metal-free click chemistry for soft tissue engineering.
25839124	1	63	theme	cell	158:161	arg1	materials					175:183	important cell scaffolding materials	148:183	important cell scaffolding materials for tissue engineering and regenerative medicine	148:232	Injectable hydrogels are important cell scaffolding materials for tissue engineering and regenerative medicine.
25839124	1	63	theme	cell	158:161	arg1	hydrogels					134:142	Injectable hydrogels	123:142	Injectable hydrogels	123:142	Injectable hydrogels are important cell scaffolding materials for tissue engineering and regenerative medicine.
25839124	4	64	theme	polysaccharide	685:698	arg1	derivatives					700:710	polysaccharide derivatives	685:710	polysaccharide derivatives	685:710	The gelation is attributed to the triazole ring formation between OB and azido groups of polysaccharide derivatives.
25839124	4	65	theme	OB	662:663	arg1	groups					675:680	OB and azido groups	662:680	OB and azido groups of polysaccharide derivatives	662:710	The gelation is attributed to the triazole ring formation between OB and azido groups of polysaccharide derivatives.
25839124	4	65	theme	OB	662:663	arg1	derivatives					700:710	polysaccharide derivatives	685:710	polysaccharide derivatives	685:710	The gelation is attributed to the triazole ring formation between OB and azido groups of polysaccharide derivatives.
25839124	0	66	theme	metal-free	67:76	arg1	chemistry					84:92	a metal-free click chemistry	65:92	a metal-free click chemistry for soft tissue engineering	65:120	Cytocompatible in situ forming chitosan/hyaluronan hydrogels via a metal-free click chemistry for soft tissue engineering.
25839124	3	67	theme	metal-free	443:452	arg1	reaction					460:467	the metal-free click reaction	439:467	the metal-free click reaction	439:467	For the metal-free click reaction, chitosan and hyaluronan were modified with oxanorbornadiene (OB) and 11-azido-3,6,9-trioxaundecan-1-amine (AA), respectively.
25839124	1	68	theme	scaffolding	163:173	arg1	materials					175:183	important cell scaffolding materials	148:183	important cell scaffolding materials for tissue engineering and regenerative medicine	148:232	Injectable hydrogels are important cell scaffolding materials for tissue engineering and regenerative medicine.
25839124	1	68	theme	scaffolding	163:173	arg1	hydrogels					134:142	Injectable hydrogels	123:142	Injectable hydrogels	123:142	Injectable hydrogels are important cell scaffolding materials for tissue engineering and regenerative medicine.
25839124	6	69	dep	in	894:895	arg1	vitro					897:901	vitro	897:901	vitro	897:901	The in vitro gelation, morphologies, equilibrium swelling, compressive modulus and degradation of the composite hydrogels were examined.
27760213	5	0	theme	leptin	769:774	arg1	sensitivity					776:786	leptin sensitivity	769:786	leptin sensitivity	769:786	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	3	1	theme	free	649:652	arg1	access					654:659	free access	649:659	free access to inulin (10g/L in drinking water)	649:695	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	3	2	theme	Wistar	519:524	arg1	rats					526:529	Wistar rats	519:529	Wistar rats	519:529	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	5	3	from	composition	886:896	arg1	colon					913:917	the colon	909:917	the colon	909:917	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	3	from	composition	886:896	arg1	liver					825:829	liver	825:829	liver	825:829	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	1	4	theme	metabolic	249:257	arg1	disorders					259:267	metabolic disorders	249:267	metabolic disorders	249:267	Increasing evidence indicates that chlorpyrifos (CPF), an organophosphorus insecticide, is involved in metabolic disorders.
27760213	10	5	with	comparable	1385:1394	arg1	controls					1405:1412	the controls	1401:1412	the controls	1401:1412	Inulin restored the levels of some metabolic parameters in CPF groups to ranges comparable with the controls.
27760213	13	6	theme	rats	1791:1794	arg1	disorders					1778:1786	adult metabolic disorders	1762:1786	adult metabolic disorders of rats exposed to CPF during development	1762:1828	By modulating microbiota population and fermentative activity, inulin corrected adult metabolic disorders of rats exposed to CPF during development.
27760213	5	7	theme	Metabolic	750:758	arg1	profile					760:766	Metabolic profile	750:766	Metabolic profile	750:766	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	3	8	from	10g/L	672:676	arg1	water					690:694	drinking water	681:694	drinking water	681:694	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	7	9	from	PND	1102:1104	arg1	BW					1096:1097	the BW	1092:1097	the BW at PND 60 to control levels	1092:1125	Inulin supplementation restored the BW at PND 60 to control levels.
27760213	0	10	theme	Offspring	130:138	arg1	Rats					140:143	Offspring Rats	130:143	Offspring Rats	130:143	Inulin Supplementation Lowered the Metabolic Defects of Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage in Offspring Rats.
27760213	5	11	theme	insulin	789:795	arg1	IR					807:808	IR	807:808	IR	807:808	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	11	theme	insulin	789:795	arg1	receptor					797:804	insulin receptor	789:804	insulin receptor (IR) expression in liver	789:829	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	3	12	theme	CPF	584:586	arg1	pesticide					588:596	CPF pesticide	584:596	CPF pesticide	584:596	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	5	13	theme	microbiota	836:845	arg1	composition					847:857	gut microbiota composition	832:857	gut microbiota composition	832:857	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	12	14	from	adulthood	1671:1679	arg1	evident					1660:1666	evident	1660:1666	evident	1660:1666	Our data indicate that developmental exposure to CPF interferes with metabolism with dose related effects evident at adulthood.
27760213	8	15	theme	receptor	1169:1176	arg1	β					1178:1178	insulin receptor β	1161:1178	insulin receptor β	1161:1178	Hyperinsulinemia and decrease in insulin receptor β in liver were seen in CPF1 exposed rats.
27760213	13	16	theme	metabolic	1768:1776	arg1	disorders					1778:1786	adult metabolic disorders	1762:1786	adult metabolic disorders of rats exposed to CPF during development	1762:1828	By modulating microbiota population and fermentative activity, inulin corrected adult metabolic disorders of rats exposed to CPF during development.
27760213	9	17	theme	insulin	1264:1270	arg1	level					1272:1276	insulin level	1264:1276	insulin level	1264:1276	In contrast, hyperglycemia and decrease in insulin level were found in CPF3.5 rats.
27760213	10	18	from	levels	1325:1330	arg1	groups					1368:1373	CPF groups	1364:1373	CPF groups	1364:1373	Inulin restored the levels of some metabolic parameters in CPF groups to ranges comparable with the controls.
27760213	0	19	from	Gestation	96:104	arg1	Chlorpyrifos					78:89	Chlorpyrifos	78:89	Chlorpyrifos from Gestation	78:104	Inulin Supplementation Lowered the Metabolic Defects of Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage in Offspring Rats.
27760213	0	20	theme	Exposure	66:73	arg1	Defects					45:51	the Metabolic Defects	31:51	the Metabolic Defects of Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage in Offspring Rats	31:143	Inulin Supplementation Lowered the Metabolic Defects of Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage in Offspring Rats.
27760213	1	21	theme	Increasing	146:155	arg1	evidence					157:164	Increasing evidence	146:164	Increasing evidence	146:164	Increasing evidence indicates that chlorpyrifos (CPF), an organophosphorus insecticide, is involved in metabolic disorders.
27760213	3	22	from	gestation	537:545	arg1	groups					509:514	5 groups	507:514	5 groups	507:514	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	3	22	from	gestation	537:545	arg1	rats					526:529	Wistar rats	519:529	Wistar rats	519:529	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	3	23	dep	doses	575:579	arg1	mg/kg/day					601:609	1 mg/kg/day	599:609	1 mg/kg/day (CPF1)	599:616	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	3	23	dep	doses	575:579	arg1	mg/kg/day					625:633	3.5 mg/kg/day	621:633	3.5 mg/kg/day (CPF3.5)	621:642	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	3	23	dep	doses	575:579	arg1	doses					575:579	two doses	571:579	two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5)	571:642	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	3	23	dep	doses	575:579	arg1	CPF3.5					636:641	CPF3.5	636:641	CPF3.5	636:641	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	3	23	dep	doses	575:579	arg1	CPF1					612:615	CPF1	612:615	CPF1	612:615	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	5	24	from	profile	760:766	arg1	colon					913:917	the colon	909:917	the colon	909:917	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	24	from	profile	760:766	arg1	liver					825:829	liver	825:829	liver	825:829	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	25	from	expression	811:820	arg1	liver					825:829	liver	825:829	liver	825:829	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	25	from	expression	811:820	arg1	colon					913:917	the colon	909:917	the colon	909:917	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	14	26	theme	continuous	1963:1972	arg1	exposure					1984:1991	a continuous pesticide exposure	1961:1991	a continuous pesticide exposure	1961:1991	Prebiotics supply may be thus considered as a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure.
27760213	8	27	theme	exposed	1207:1213	arg1	rats					1215:1218	CPF1 exposed rats	1202:1218	CPF1 exposed rats	1202:1218	Hyperinsulinemia and decrease in insulin receptor β in liver were seen in CPF1 exposed rats.
27760213	0	28	theme	Young	109:113	arg1	Stage					121:125	Young Adult Stage	109:125	Young Adult Stage	109:125	Inulin Supplementation Lowered the Metabolic Defects of Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage in Offspring Rats.
27760213	5	29	from	composition	847:857	arg1	colon					913:917	the colon	909:917	the colon	909:917	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	29	from	composition	847:857	arg1	liver					825:829	liver	825:829	liver	825:829	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	13	30	theme	fermentative	1722:1733	arg1	activity					1735:1742	fermentative activity	1722:1742	fermentative activity	1722:1742	By modulating microbiota population and fermentative activity, inulin corrected adult metabolic disorders of rats exposed to CPF during development.
27760213	2	31	theme	microbiota	388:397	arg1	composition					399:409	microbiota composition	388:409	microbiota composition	388:409	We assess the hypothesis whether supplementation with prebiotics from gestation to adulthood, through a modulation of microbiota composition and fermentative activity, alleviates CPF induced metabolic disorders of 60 days old offspring.
27760213	0	32	theme	Inulin	0:5	arg1	Supplementation					7:21	Inulin Supplementation	0:21	Inulin Supplementation	0:21	Inulin Supplementation Lowered the Metabolic Defects of Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage in Offspring Rats.
27760213	5	33	theme	chain	869:873	arg1	SCFAs					899:903	SCFAs	899:903	SCFAs	899:903	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	33	theme	chain	869:873	arg1	composition					886:896	short chain fatty acid composition	863:896	short chain fatty acid composition (SCFAs)	863:904	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	2	34	theme	induced	453:459	arg1	disorders					471:479	CPF induced metabolic disorders	449:479	CPF induced metabolic disorders of 60 days old offspring	449:504	We assess the hypothesis whether supplementation with prebiotics from gestation to adulthood, through a modulation of microbiota composition and fermentative activity, alleviates CPF induced metabolic disorders of 60 days old offspring.
27760213	10	35	theme	metabolic	1340:1348	arg1	parameters					1350:1359	some metabolic parameters	1335:1359	some metabolic parameters	1335:1359	Inulin restored the levels of some metabolic parameters in CPF groups to ranges comparable with the controls.
27760213	5	36	theme	acid	881:884	arg1	SCFAs					899:903	SCFAs	899:903	SCFAs	899:903	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	36	theme	acid	881:884	arg1	composition					886:896	short chain fatty acid composition	863:896	short chain fatty acid composition (SCFAs)	863:904	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	2	37	theme	old	492:494	arg1	offspring					496:504	60 days old offspring	484:504	60 days old offspring	484:504	We assess the hypothesis whether supplementation with prebiotics from gestation to adulthood, through a modulation of microbiota composition and fermentative activity, alleviates CPF induced metabolic disorders of 60 days old offspring.
27760213	2	38	theme	activity	428:435	arg1	modulation					374:383	a modulation	372:383	a modulation of microbiota composition and fermentative activity	372:435	We assess the hypothesis whether supplementation with prebiotics from gestation to adulthood, through a modulation of microbiota composition and fermentative activity, alleviates CPF induced metabolic disorders of 60 days old offspring.
27760213	0	39	theme	Metabolic	35:43	arg1	Defects					45:51	the Metabolic Defects	31:51	the Metabolic Defects of Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage in Offspring Rats	31:143	Inulin Supplementation Lowered the Metabolic Defects of Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage in Offspring Rats.
27760213	9	40	from	decrease	1252:1259	arg1	level					1272:1276	insulin level	1264:1276	insulin level	1264:1276	In contrast, hyperglycemia and decrease in insulin level were found in CPF3.5 rats.
27760213	5	41	theme	postnatal	937:945	arg1	day					947:949	postnatal day 60	937:952	postnatal day 60 in the offspring (PND 60)	937:978	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	41	theme	postnatal	937:945	arg1	PND					972:974	PND 60	972:977	PND 60	972:977	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	6	42	theme	birth	1010:1014	arg1	BW					1029:1030	BW	1029:1030	BW	1029:1030	CPF3.5 increased offspring's birth body weight (BW) but decreased BW at PND60.
27760213	6	42	theme	birth	1010:1014	arg1	weight					1021:1026	offspring's birth body weight	998:1026	offspring's birth body weight (BW)	998:1031	CPF3.5 increased offspring's birth body weight (BW) but decreased BW at PND60.
27760213	11	43	theme	bacterial	1425:1433	arg1	population					1435:1444	The total bacterial population	1415:1444	The total bacterial population	1415:1444	The total bacterial population, short chain fatty acid (SCFA) production and butyrate levels were enhanced in CPF groups receiving inulin.
27760213	9	44	from	hyperglycemia	1234:1246	arg1	level					1272:1276	insulin level	1264:1276	insulin level	1264:1276	In contrast, hyperglycemia and decrease in insulin level were found in CPF3.5 rats.
27760213	11	45	theme	acid	1465:1468	arg1	production					1477:1486	short chain fatty acid (SCFA) production	1447:1486	short chain fatty acid (SCFA) production	1447:1486	The total bacterial population, short chain fatty acid (SCFA) production and butyrate levels were enhanced in CPF groups receiving inulin.
27760213	3	46	theme	rats	526:529	arg1	groups					509:514	5 groups	507:514	5 groups	507:514	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	3	46	theme	rats	526:529	arg1	rats					526:529	Wistar rats	519:529	Wistar rats	519:529	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	12	47	theme	dose	1639:1642	arg1	effects					1652:1658	dose related effects	1639:1658	dose related effects evident at adulthood	1639:1679	Our data indicate that developmental exposure to CPF interferes with metabolism with dose related effects evident at adulthood.
27760213	7	48	theme	control	1112:1118	arg1	levels					1120:1125	control levels	1112:1125	control levels	1112:1125	Inulin supplementation restored the BW at PND 60 to control levels.
27760213	11	49	theme	chain	1453:1457	arg1	SCFA					1471:1474	SCFA	1471:1474	SCFA	1471:1474	The total bacterial population, short chain fatty acid (SCFA) production and butyrate levels were enhanced in CPF groups receiving inulin.
27760213	11	49	theme	chain	1453:1457	arg1	acid					1465:1468	chain fatty acid	1453:1468	short chain fatty acid (SCFA) production	1447:1486	The total bacterial population, short chain fatty acid (SCFA) production and butyrate levels were enhanced in CPF groups receiving inulin.
27760213	9	50	located	found	1283:1287	arg1	rats					1299:1302	CPF3.5 rats	1292:1302	CPF3.5 rats	1292:1302	In contrast, hyperglycemia and decrease in insulin level were found in CPF3.5 rats.
27760213	9	50	located	found	1283:1287	arg2	decrease					1252:1259	decrease	1252:1259	decrease	1252:1259	In contrast, hyperglycemia and decrease in insulin level were found in CPF3.5 rats.
27760213	9	50	located	found	1283:1287	arg2	hyperglycemia					1234:1246	hyperglycemia	1234:1246	hyperglycemia	1234:1246	In contrast, hyperglycemia and decrease in insulin level were found in CPF3.5 rats.
27760213	9	50	located	found	1283:1287	arg1	contrast					1224:1231	contrast	1224:1231	contrast	1224:1231	In contrast, hyperglycemia and decrease in insulin level were found in CPF3.5 rats.
27760213	2	51	from	gestation	340:348	arg1	prebiotics					324:333	prebiotics	324:333	prebiotics from gestation to adulthood	324:361	We assess the hypothesis whether supplementation with prebiotics from gestation to adulthood, through a modulation of microbiota composition and fermentative activity, alleviates CPF induced metabolic disorders of 60 days old offspring.
27760213	3	52	theme	drinking	681:688	arg1	water					690:694	drinking water	681:694	drinking water	681:694	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	5	53	theme	receptor	797:804	arg1	expression					811:820	insulin receptor (IR) expression	789:820	insulin receptor (IR) expression in liver	789:829	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	11	54	theme	butyrate	1492:1499	arg1	levels					1501:1506	butyrate levels	1492:1506	butyrate levels	1492:1506	The total bacterial population, short chain fatty acid (SCFA) production and butyrate levels were enhanced in CPF groups receiving inulin.
27760213	9	55	theme	CPF3.5	1292:1297	arg1	rats					1299:1302	CPF3.5 rats	1292:1302	CPF3.5 rats	1292:1302	In contrast, hyperglycemia and decrease in insulin level were found in CPF3.5 rats.
27760213	12	56	theme	developmental	1577:1589	arg1	exposure					1591:1598	developmental exposure	1577:1598	developmental exposure to CPF	1577:1605	Our data indicate that developmental exposure to CPF interferes with metabolism with dose related effects evident at adulthood.
27760213	8	57	dep	exposed	1207:1213	arg1	CPF1					1202:1205	CPF1	1202:1205	CPF1	1202:1205	Hyperinsulinemia and decrease in insulin receptor β in liver were seen in CPF1 exposed rats.
27760213	3	58	theme	pesticide	588:596	arg1	mg/kg/day					601:609	1 mg/kg/day	599:609	1 mg/kg/day (CPF1)	599:616	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	3	58	theme	pesticide	588:596	arg1	mg/kg/day					625:633	3.5 mg/kg/day	621:633	3.5 mg/kg/day (CPF3.5)	621:642	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	3	58	theme	pesticide	588:596	arg1	doses					575:579	two doses	571:579	two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5)	571:642	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	5	59	theme	gut	832:834	arg1	composition					847:857	gut microbiota composition	832:857	gut microbiota composition	832:857	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	13	60	theme	adult	1762:1766	arg1	disorders					1778:1786	adult metabolic disorders	1762:1786	adult metabolic disorders of rats exposed to CPF during development	1762:1828	By modulating microbiota population and fermentative activity, inulin corrected adult metabolic disorders of rats exposed to CPF during development.
27760213	5	61	from	sensitivity	776:786	arg1	colon					913:917	the colon	909:917	the colon	909:917	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	61	from	sensitivity	776:786	arg1	liver					825:829	liver	825:829	liver	825:829	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	8	62	theme	insulin	1161:1167	arg1	β					1178:1178	insulin receptor β	1161:1178	insulin receptor β	1161:1178	Hyperinsulinemia and decrease in insulin receptor β in liver were seen in CPF1 exposed rats.
27760213	8	63	from	Hyperinsulinemia	1128:1143	arg1	liver					1183:1187	liver	1183:1187	liver	1183:1187	Hyperinsulinemia and decrease in insulin receptor β in liver were seen in CPF1 exposed rats.
27760213	8	63	from	Hyperinsulinemia	1128:1143	arg1	β					1178:1178	insulin receptor β	1161:1178	insulin receptor β	1161:1178	Hyperinsulinemia and decrease in insulin receptor β in liver were seen in CPF1 exposed rats.
27760213	5	64	from	day	947:949	arg1	offspring					961:969	the offspring	957:969	the offspring	957:969	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	14	65	theme	nutritional	1883:1893	arg1	supply					1842:1847	Prebiotics supply	1831:1847	Prebiotics supply	1831:1847	Prebiotics supply may be thus considered as a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure.
27760213	14	65	theme	nutritional	1883:1893	arg1	strategy					1895:1902	a novel nutritional strategy	1875:1902	a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure	1875:1991	Prebiotics supply may be thus considered as a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure.
27760213	2	66	with	supplementation	303:317	arg1	prebiotics					324:333	prebiotics	324:333	prebiotics from gestation to adulthood	324:361	We assess the hypothesis whether supplementation with prebiotics from gestation to adulthood, through a modulation of microbiota composition and fermentative activity, alleviates CPF induced metabolic disorders of 60 days old offspring.
27760213	2	67	theme	offspring	496:504	arg1	disorders					471:479	CPF induced metabolic disorders	449:479	CPF induced metabolic disorders of 60 days old offspring	449:504	We assess the hypothesis whether supplementation with prebiotics from gestation to adulthood, through a modulation of microbiota composition and fermentative activity, alleviates CPF induced metabolic disorders of 60 days old offspring.
27760213	7	68	theme	Inulin	1060:1065	arg1	supplementation					1067:1081	Inulin supplementation	1060:1081	Inulin supplementation	1060:1081	Inulin supplementation restored the BW at PND 60 to control levels.
27760213	14	69	theme	novel	1877:1881	arg1	supply					1842:1847	Prebiotics supply	1831:1847	Prebiotics supply	1831:1847	Prebiotics supply may be thus considered as a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure.
27760213	14	69	theme	novel	1877:1881	arg1	strategy					1895:1902	a novel nutritional strategy	1875:1902	a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure	1875:1991	Prebiotics supply may be thus considered as a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure.
27760213	14	70	theme	pesticide	1974:1982	arg1	exposure					1984:1991	a continuous pesticide exposure	1961:1991	a continuous pesticide exposure	1961:1991	Prebiotics supply may be thus considered as a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure.
27760213	4	71	theme	male	703:706	arg1	pups					708:711	Then male pups	698:711	Then male pups	698:711	Then male pups received the same treatment as dams.
27760213	13	72	theme	microbiota	1696:1705	arg1	population					1707:1716	microbiota population	1696:1716	microbiota population	1696:1716	By modulating microbiota population and fermentative activity, inulin corrected adult metabolic disorders of rats exposed to CPF during development.
27760213	0	73	theme	Adult	115:119	arg1	Stage					121:125	Young Adult Stage	109:125	Young Adult Stage	109:125	Inulin Supplementation Lowered the Metabolic Defects of Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage in Offspring Rats.
27760213	2	74	dep	induced	453:459	arg1	CPF					449:451	CPF	449:451	CPF	449:451	We assess the hypothesis whether supplementation with prebiotics from gestation to adulthood, through a modulation of microbiota composition and fermentative activity, alleviates CPF induced metabolic disorders of 60 days old offspring.
27760213	8	75	from	decrease	1149:1156	arg1	liver					1183:1187	liver	1183:1187	liver	1183:1187	Hyperinsulinemia and decrease in insulin receptor β in liver were seen in CPF1 exposed rats.
27760213	8	75	from	decrease	1149:1156	arg1	β					1178:1178	insulin receptor β	1161:1178	insulin receptor β	1161:1178	Hyperinsulinemia and decrease in insulin receptor β in liver were seen in CPF1 exposed rats.
27760213	0	76	from	Defects	45:51	arg1	Rats					140:143	Offspring Rats	130:143	Offspring Rats	130:143	Inulin Supplementation Lowered the Metabolic Defects of Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage in Offspring Rats.
27760213	2	77	theme	composition	399:409	arg1	modulation					374:383	a modulation	372:383	a modulation of microbiota composition and fermentative activity	372:435	We assess the hypothesis whether supplementation with prebiotics from gestation to adulthood, through a modulation of microbiota composition and fermentative activity, alleviates CPF induced metabolic disorders of 60 days old offspring.
27760213	5	78	theme	short	863:867	arg1	SCFAs					899:903	SCFAs	899:903	SCFAs	899:903	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	78	theme	short	863:867	arg1	composition					886:896	short chain fatty acid composition	863:896	short chain fatty acid composition (SCFAs)	863:904	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	2	79	theme	metabolic	461:469	arg1	disorders					471:479	CPF induced metabolic disorders	449:479	CPF induced metabolic disorders of 60 days old offspring	449:504	We assess the hypothesis whether supplementation with prebiotics from gestation to adulthood, through a modulation of microbiota composition and fermentative activity, alleviates CPF induced metabolic disorders of 60 days old offspring.
27760213	5	80	theme	fatty	875:879	arg1	SCFAs					899:903	SCFAs	899:903	SCFAs	899:903	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	5	80	theme	fatty	875:879	arg1	composition					886:896	short chain fatty acid composition	863:896	short chain fatty acid composition (SCFAs)	863:904	Metabolic profile, leptin sensitivity, insulin receptor (IR) expression in liver, gut microbiota composition and short chain fatty acid composition (SCFAs) in the colon, were analyzed at postnatal day 60 in the offspring (PND 60).
27760213	11	81	theme	CPF	1525:1527	arg1	groups					1529:1534	CPF groups	1525:1534	CPF groups receiving inulin	1525:1551	The total bacterial population, short chain fatty acid (SCFA) production and butyrate levels were enhanced in CPF groups receiving inulin.
27760213	3	82	dep	inulin	664:669	arg1	10g/L					672:676	10g/L	672:676	10g/L in drinking water	672:694	5 groups of Wistar rats, from gestation until weaning, received two doses of CPF pesticide: 1 mg/kg/day (CPF1) or 3.5 mg/kg/day (CPF3.5) with free access to inulin (10g/L in drinking water).
27760213	14	83	theme	Prebiotics	1831:1840	arg1	supply					1842:1847	Prebiotics supply	1831:1847	Prebiotics supply	1831:1847	Prebiotics supply may be thus considered as a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure.
27760213	14	83	theme	Prebiotics	1831:1840	arg1	strategy					1895:1902	a novel nutritional strategy	1875:1902	a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure	1875:1991	Prebiotics supply may be thus considered as a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure.
27760213	14	84	theme	insulin	1918:1924	arg1	resistance					1926:1935	insulin resistance	1918:1935	insulin resistance	1918:1935	Prebiotics supply may be thus considered as a novel nutritional strategy to counteract insulin resistance and diabetes induced by a continuous pesticide exposure.
27760213	2	85	theme	fermentative	415:426	arg1	activity					428:435	fermentative activity	415:435	fermentative activity	415:435	We assess the hypothesis whether supplementation with prebiotics from gestation to adulthood, through a modulation of microbiota composition and fermentative activity, alleviates CPF induced metabolic disorders of 60 days old offspring.
27760213	0	86	theme	Prolonged	56:64	arg1	Exposure					66:73	Prolonged Exposure	56:73	Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage	56:125	Inulin Supplementation Lowered the Metabolic Defects of Prolonged Exposure to Chlorpyrifos from Gestation to Young Adult Stage in Offspring Rats.
27760213	12	87	theme	related	1644:1650	arg1	effects					1652:1658	dose related effects	1639:1658	dose related effects evident at adulthood	1639:1679	Our data indicate that developmental exposure to CPF interferes with metabolism with dose related effects evident at adulthood.
27760213	6	88	theme	body	1016:1019	arg1	BW					1029:1030	BW	1029:1030	BW	1029:1030	CPF3.5 increased offspring's birth body weight (BW) but decreased BW at PND60.
27760213	6	88	theme	body	1016:1019	arg1	weight					1021:1026	offspring's birth body weight	998:1026	offspring's birth body weight (BW)	998:1031	CPF3.5 increased offspring's birth body weight (BW) but decreased BW at PND60.
27760213	10	89	theme	CPF	1364:1366	arg1	groups					1368:1373	CPF groups	1364:1373	CPF groups	1364:1373	Inulin restored the levels of some metabolic parameters in CPF groups to ranges comparable with the controls.
27760213	11	90	theme	total	1419:1423	arg1	population					1435:1444	The total bacterial population	1415:1444	The total bacterial population	1415:1444	The total bacterial population, short chain fatty acid (SCFA) production and butyrate levels were enhanced in CPF groups receiving inulin.
27760213	12	91	theme	evident	1660:1666	arg1	effects					1652:1658	dose related effects	1639:1658	dose related effects evident at adulthood	1639:1679	Our data indicate that developmental exposure to CPF interferes with metabolism with dose related effects evident at adulthood.
27760213	10	92	theme	parameters	1350:1359	arg1	levels					1325:1330	the levels	1321:1330	the levels of some metabolic parameters in CPF groups	1321:1373	Inulin restored the levels of some metabolic parameters in CPF groups to ranges comparable with the controls.
27760213	4	93	theme	same	726:729	arg1	treatment					731:739	the same treatment	722:739	the same treatment	722:739	Then male pups received the same treatment as dams.
27760213	11	94	theme	fatty	1459:1463	arg1	SCFA					1471:1474	SCFA	1471:1474	SCFA	1471:1474	The total bacterial population, short chain fatty acid (SCFA) production and butyrate levels were enhanced in CPF groups receiving inulin.
27760213	11	94	theme	fatty	1459:1463	arg1	acid					1465:1468	chain fatty acid	1453:1468	short chain fatty acid (SCFA) production	1447:1486	The total bacterial population, short chain fatty acid (SCFA) production and butyrate levels were enhanced in CPF groups receiving inulin.
27760213	1	95	theme	organophosphorus	204:219	arg1	insecticide					221:231	an organophosphorus insecticide	201:231	an organophosphorus insecticide	201:231	Increasing evidence indicates that chlorpyrifos (CPF), an organophosphorus insecticide, is involved in metabolic disorders.
27760213	1	95	theme	organophosphorus	204:219	arg1	chlorpyrifos					181:192	chlorpyrifos	181:192	chlorpyrifos (CPF)	181:198	Increasing evidence indicates that chlorpyrifos (CPF), an organophosphorus insecticide, is involved in metabolic disorders.
27760213	10	96	theme	comparable	1385:1394	arg1	ranges					1378:1383	ranges	1378:1383	ranges comparable with the controls	1378:1412	Inulin restored the levels of some metabolic parameters in CPF groups to ranges comparable with the controls.
27760213	11	97	theme	short	1447:1451	arg1	production					1477:1486	short chain fatty acid (SCFA) production	1447:1486	short chain fatty acid (SCFA) production	1447:1486	The total bacterial population, short chain fatty acid (SCFA) production and butyrate levels were enhanced in CPF groups receiving inulin.
26686157	4	0	theme	CN	518:519	arg1	linkages					521:528	CN linkages	518:528	CN linkages	518:528	The results indicate that the hydrogen bond interactions and CN linkages between gelatin and DCMC can be formed.
26686157	5	1	theme	blood	666:670	arg1	compatibility					672:684	blood compatibility	666:684	blood compatibility	666:684	The addition of DCMC can efficiently improve the mechanical properties, enzymatic stability and blood compatibility of the hydrogel fibers.
26686157	7	2	theme	hydrogel	910:917	arg1	fibers					919:924	the composite hydrogel fibers	896:924	the composite hydrogel fibers	896:924	Moreover, the composite hydrogel fibers present three-dimensional structure, porous networks and low cytotoxicity.
26686157	6	3	theme	undesired	840:848	arg1	reduction					850:858	undesired reduction	840:858	undesired reduction in mechanical properties	840:883	Crosslinking with DCMC can reduce the degree of swelling of the hydrogel fibers, which is beneficial for hydrogel fibers to avoid undesired reduction in mechanical properties.
26686157	6	4	theme	mechanical	863:872	arg1	properties					874:883	mechanical properties	863:883	mechanical properties	863:883	Crosslinking with DCMC can reduce the degree of swelling of the hydrogel fibers, which is beneficial for hydrogel fibers to avoid undesired reduction in mechanical properties.
26686157	9	5	theme	developed	1098:1106	arg1	fibers					1127:1132	The developed composite hydrogel fibers	1094:1132	The developed composite hydrogel fibers	1094:1132	The developed composite hydrogel fibers can be well-suited for biomedical applications such as wound dressings.
26686157	6	6	theme	hydrogel	815:822	arg1	fibers					824:829	hydrogel fibers	815:829	hydrogel fibers	815:829	Crosslinking with DCMC can reduce the degree of swelling of the hydrogel fibers, which is beneficial for hydrogel fibers to avoid undesired reduction in mechanical properties.
26686157	9	7	theme	composite	1108:1116	arg1	fibers					1127:1132	The developed composite hydrogel fibers	1094:1132	The developed composite hydrogel fibers	1094:1132	The developed composite hydrogel fibers can be well-suited for biomedical applications such as wound dressings.
26686157	0	8	theme	wound	114:118	arg1	dressings					120:128	wound dressings	114:128	wound dressings	114:128	Biological properties of dialdehyde carboxymethyl cellulose crosslinked gelatin-PEG composite hydrogel fibers for wound dressings.
26686157	4	9	theme	bond	496:499	arg1	interactions					501:512	hydrogen bond interactions	487:512	hydrogen bond interactions	487:512	The results indicate that the hydrogen bond interactions and CN linkages between gelatin and DCMC can be formed.
26686157	5	10	theme	mechanical	619:628	arg1	properties					630:639	the mechanical properties	615:639	the mechanical properties	615:639	The addition of DCMC can efficiently improve the mechanical properties, enzymatic stability and blood compatibility of the hydrogel fibers.
26686157	9	11	theme	hydrogel	1118:1125	arg1	fibers					1127:1132	The developed composite hydrogel fibers	1094:1132	The developed composite hydrogel fibers	1094:1132	The developed composite hydrogel fibers can be well-suited for biomedical applications such as wound dressings.
26686157	8	12	dep	biomaterials	1071:1082	arg1	fixation					1084:1091	fixation	1084:1091	biomaterials fixation	1071:1091	The study suggests that DCMC is an effective crosslinking reagent for biomaterials fixation.
26686157	4	13	theme	hydrogen	487:494	arg1	interactions					501:512	hydrogen bond interactions	487:512	hydrogen bond interactions	487:512	The results indicate that the hydrogen bond interactions and CN linkages between gelatin and DCMC can be formed.
26686157	0	14	theme	cellulose	50:58	arg1	properties					11:20	Biological properties	0:20	Biological properties of dialdehyde carboxymethyl cellulose	0:58	Biological properties of dialdehyde carboxymethyl cellulose crosslinked gelatin-PEG composite hydrogel fibers for wound dressings.
26686157	0	15	theme	Biological	0:9	arg1	properties					11:20	Biological properties	0:20	Biological properties of dialdehyde carboxymethyl cellulose	0:58	Biological properties of dialdehyde carboxymethyl cellulose crosslinked gelatin-PEG composite hydrogel fibers for wound dressings.
26686157	7	16	theme	low	983:985	arg1	cytotoxicity					987:998	low cytotoxicity	983:998	low cytotoxicity	983:998	Moreover, the composite hydrogel fibers present three-dimensional structure, porous networks and low cytotoxicity.
26686157	5	17	theme	DCMC	586:589	arg1	addition					574:581	The addition	570:581	The addition of DCMC	570:589	The addition of DCMC can efficiently improve the mechanical properties, enzymatic stability and blood compatibility of the hydrogel fibers.
26686157	3	18	theme	hydrogel	405:412	arg1	fibers					414:419	the hydrogel fibers	401:419	the hydrogel fibers for wound dressings	401:439	Then the biological properties of the hydrogel fibers for wound dressings were evaluated.
26686157	8	19	theme	crosslinking	1046:1057	arg1	reagent					1059:1065	an effective crosslinking reagent	1033:1065	an effective crosslinking reagent for biomaterials fixation	1033:1091	The study suggests that DCMC is an effective crosslinking reagent for biomaterials fixation.
26686157	8	19	theme	crosslinking	1046:1057	arg1	DCMC					1025:1028	DCMC	1025:1028	DCMC	1025:1028	The study suggests that DCMC is an effective crosslinking reagent for biomaterials fixation.
26686157	0	20	theme	dialdehyde	25:34	arg1	cellulose					50:58	dialdehyde carboxymethyl cellulose	25:58	dialdehyde carboxymethyl cellulose	25:58	Biological properties of dialdehyde carboxymethyl cellulose crosslinked gelatin-PEG composite hydrogel fibers for wound dressings.
26686157	4	21	dep	interactions	501:512	arg1	the					483:485	the	483:485	the	483:485	The results indicate that the hydrogen bond interactions and CN linkages between gelatin and DCMC can be formed.
26686157	7	22	theme	porous	963:968	arg1	networks					970:977	porous networks	963:977	porous networks	963:977	Moreover, the composite hydrogel fibers present three-dimensional structure, porous networks and low cytotoxicity.
26686157	1	23	theme	Gelatin-based	131:143	arg1	fibers					164:169	Gelatin-based composite hydrogel fibers	131:169	Gelatin-based composite hydrogel fibers	131:169	Gelatin-based composite hydrogel fibers were prepared by gel-spinning with PEG6000 as the modifier.
26686157	9	24	theme	biomedical	1157:1166	arg1	dressings					1195:1203	wound dressings	1189:1203	wound dressings	1189:1203	The developed composite hydrogel fibers can be well-suited for biomedical applications such as wound dressings.
26686157	9	24	theme	biomedical	1157:1166	arg1	applications					1168:1179	biomedical applications	1157:1179	biomedical applications such as wound dressings	1157:1203	The developed composite hydrogel fibers can be well-suited for biomedical applications such as wound dressings.
26686157	3	25	theme	wound	425:429	arg1	dressings					431:439	wound dressings	425:439	wound dressings	425:439	Then the biological properties of the hydrogel fibers for wound dressings were evaluated.
26686157	2	26	theme	hydrogel	350:357	arg1	fibers					359:364	the composite hydrogel fibers	336:364	the composite hydrogel fibers	336:364	Dialdehyde carboxymethyl cellulose (DCMC), as an ideal crosslinking reagent for protein, was used to fix the composite hydrogel fibers.
26686157	0	27	theme	carboxymethyl	36:48	arg1	cellulose					50:58	dialdehyde carboxymethyl cellulose	25:58	dialdehyde carboxymethyl cellulose	25:58	Biological properties of dialdehyde carboxymethyl cellulose crosslinked gelatin-PEG composite hydrogel fibers for wound dressings.
26686157	6	28	theme	fibers	783:788	arg1	swelling					758:765	swelling	758:765	swelling of the hydrogel fibers, which is beneficial for hydrogel fibers to avoid undesired reduction in mechanical properties	758:883	Crosslinking with DCMC can reduce the degree of swelling of the hydrogel fibers, which is beneficial for hydrogel fibers to avoid undesired reduction in mechanical properties.
26686157	3	29	theme	fibers	414:419	arg1	properties					387:396	the biological properties	372:396	the biological properties of the hydrogel fibers for wound dressings	372:439	Then the biological properties of the hydrogel fibers for wound dressings were evaluated.
26686157	5	30	theme	hydrogel	693:700	arg1	fibers					702:707	the hydrogel fibers	689:707	the hydrogel fibers	689:707	The addition of DCMC can efficiently improve the mechanical properties, enzymatic stability and blood compatibility of the hydrogel fibers.
26686157	0	31	theme	composite	84:92	arg1	fibers					103:108	gelatin-PEG composite hydrogel fibers	72:108	gelatin-PEG composite hydrogel fibers for wound dressings	72:128	Biological properties of dialdehyde carboxymethyl cellulose crosslinked gelatin-PEG composite hydrogel fibers for wound dressings.
26686157	6	32	theme	hydrogel	774:781	arg1	beneficial					800:809	beneficial	800:809	beneficial	800:809	Crosslinking with DCMC can reduce the degree of swelling of the hydrogel fibers, which is beneficial for hydrogel fibers to avoid undesired reduction in mechanical properties.
26686157	6	32	theme	hydrogel	774:781	arg1	fibers					783:788	the hydrogel fibers	770:788	the hydrogel fibers	770:788	Crosslinking with DCMC can reduce the degree of swelling of the hydrogel fibers, which is beneficial for hydrogel fibers to avoid undesired reduction in mechanical properties.
26686157	1	33	theme	composite	145:153	arg1	fibers					164:169	Gelatin-based composite hydrogel fibers	131:169	Gelatin-based composite hydrogel fibers	131:169	Gelatin-based composite hydrogel fibers were prepared by gel-spinning with PEG6000 as the modifier.
26686157	6	34	from	reduction	850:858	arg1	properties					874:883	mechanical properties	863:883	mechanical properties	863:883	Crosslinking with DCMC can reduce the degree of swelling of the hydrogel fibers, which is beneficial for hydrogel fibers to avoid undesired reduction in mechanical properties.
26686157	5	35	theme	fibers	702:707	arg1	stability					652:660	enzymatic stability	642:660	enzymatic stability	642:660	The addition of DCMC can efficiently improve the mechanical properties, enzymatic stability and blood compatibility of the hydrogel fibers.
26686157	5	35	theme	fibers	702:707	arg1	compatibility					672:684	blood compatibility	666:684	blood compatibility	666:684	The addition of DCMC can efficiently improve the mechanical properties, enzymatic stability and blood compatibility of the hydrogel fibers.
26686157	5	35	theme	fibers	702:707	arg1	properties					630:639	the mechanical properties	615:639	the mechanical properties	615:639	The addition of DCMC can efficiently improve the mechanical properties, enzymatic stability and blood compatibility of the hydrogel fibers.
26686157	5	36	theme	enzymatic	642:650	arg1	stability					652:660	enzymatic stability	642:660	enzymatic stability	642:660	The addition of DCMC can efficiently improve the mechanical properties, enzymatic stability and blood compatibility of the hydrogel fibers.
26686157	9	37	theme	wound	1189:1193	arg1	dressings					1195:1203	wound dressings	1189:1203	wound dressings	1189:1203	The developed composite hydrogel fibers can be well-suited for biomedical applications such as wound dressings.
26686157	1	38	theme	hydrogel	155:162	arg1	fibers					164:169	Gelatin-based composite hydrogel fibers	131:169	Gelatin-based composite hydrogel fibers	131:169	Gelatin-based composite hydrogel fibers were prepared by gel-spinning with PEG6000 as the modifier.
26686157	7	39	theme	three-dimensional	934:950	arg1	structure					952:960	three-dimensional structure	934:960	three-dimensional structure	934:960	Moreover, the composite hydrogel fibers present three-dimensional structure, porous networks and low cytotoxicity.
26686157	8	40	theme	effective	1036:1044	arg1	reagent					1059:1065	an effective crosslinking reagent	1033:1065	an effective crosslinking reagent for biomaterials fixation	1033:1091	The study suggests that DCMC is an effective crosslinking reagent for biomaterials fixation.
26686157	8	40	theme	effective	1036:1044	arg1	DCMC					1025:1028	DCMC	1025:1028	DCMC	1025:1028	The study suggests that DCMC is an effective crosslinking reagent for biomaterials fixation.
26686157	6	41	with	Crosslinking	710:721	arg1	DCMC					728:731	DCMC	728:731	DCMC	728:731	Crosslinking with DCMC can reduce the degree of swelling of the hydrogel fibers, which is beneficial for hydrogel fibers to avoid undesired reduction in mechanical properties.
26686157	2	42	theme	carboxymethyl	242:254	arg1	DCMC					267:270	DCMC	267:270	DCMC	267:270	Dialdehyde carboxymethyl cellulose (DCMC), as an ideal crosslinking reagent for protein, was used to fix the composite hydrogel fibers.
26686157	2	42	theme	carboxymethyl	242:254	arg1	cellulose					256:264	Dialdehyde carboxymethyl cellulose	231:264	Dialdehyde carboxymethyl cellulose (DCMC)	231:271	Dialdehyde carboxymethyl cellulose (DCMC), as an ideal crosslinking reagent for protein, was used to fix the composite hydrogel fibers.
26686157	2	43	theme	crosslinking	286:297	arg1	reagent					299:305	an ideal crosslinking reagent	277:305	an ideal crosslinking reagent for protein	277:317	Dialdehyde carboxymethyl cellulose (DCMC), as an ideal crosslinking reagent for protein, was used to fix the composite hydrogel fibers.
26686157	2	44	theme	composite	340:348	arg1	fibers					359:364	the composite hydrogel fibers	336:364	the composite hydrogel fibers	336:364	Dialdehyde carboxymethyl cellulose (DCMC), as an ideal crosslinking reagent for protein, was used to fix the composite hydrogel fibers.
26686157	2	45	used	used	324:327	arg2	cellulose					256:264	Dialdehyde carboxymethyl cellulose	231:264	Dialdehyde carboxymethyl cellulose (DCMC)	231:271	Dialdehyde carboxymethyl cellulose (DCMC), as an ideal crosslinking reagent for protein, was used to fix the composite hydrogel fibers.
26686157	2	45	used	used	324:327	arg2	DCMC					267:270	DCMC	267:270	DCMC	267:270	Dialdehyde carboxymethyl cellulose (DCMC), as an ideal crosslinking reagent for protein, was used to fix the composite hydrogel fibers.
26686157	2	46	theme	Dialdehyde	231:240	arg1	DCMC					267:270	DCMC	267:270	DCMC	267:270	Dialdehyde carboxymethyl cellulose (DCMC), as an ideal crosslinking reagent for protein, was used to fix the composite hydrogel fibers.
26686157	2	46	theme	Dialdehyde	231:240	arg1	cellulose					256:264	Dialdehyde carboxymethyl cellulose	231:264	Dialdehyde carboxymethyl cellulose (DCMC)	231:271	Dialdehyde carboxymethyl cellulose (DCMC), as an ideal crosslinking reagent for protein, was used to fix the composite hydrogel fibers.
26686157	2	47	theme	ideal	280:284	arg1	reagent					299:305	an ideal crosslinking reagent	277:305	an ideal crosslinking reagent for protein	277:317	Dialdehyde carboxymethyl cellulose (DCMC), as an ideal crosslinking reagent for protein, was used to fix the composite hydrogel fibers.
26686157	6	48	theme	swelling	758:765	arg1	degree					748:753	the degree	744:753	the degree of swelling of the hydrogel fibers, which is beneficial for hydrogel fibers to avoid undesired reduction in mechanical properties	744:883	Crosslinking with DCMC can reduce the degree of swelling of the hydrogel fibers, which is beneficial for hydrogel fibers to avoid undesired reduction in mechanical properties.
26686157	0	49	theme	hydrogel	94:101	arg1	fibers					103:108	gelatin-PEG composite hydrogel fibers	72:108	gelatin-PEG composite hydrogel fibers for wound dressings	72:128	Biological properties of dialdehyde carboxymethyl cellulose crosslinked gelatin-PEG composite hydrogel fibers for wound dressings.
26686157	0	50	theme	gelatin-PEG	72:82	arg1	fibers					103:108	gelatin-PEG composite hydrogel fibers	72:108	gelatin-PEG composite hydrogel fibers for wound dressings	72:128	Biological properties of dialdehyde carboxymethyl cellulose crosslinked gelatin-PEG composite hydrogel fibers for wound dressings.
26686157	7	51	theme	composite	900:908	arg1	fibers					919:924	the composite hydrogel fibers	896:924	the composite hydrogel fibers	896:924	Moreover, the composite hydrogel fibers present three-dimensional structure, porous networks and low cytotoxicity.
26686157	3	52	theme	biological	376:385	arg1	properties					387:396	the biological properties	372:396	the biological properties of the hydrogel fibers for wound dressings	372:439	Then the biological properties of the hydrogel fibers for wound dressings were evaluated.
29273221	11	0	theme	engineering	1426:1436	arg1	accomplishments					1438:1452	established tissue engineering accomplishments	1407:1452	established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage	1407:1554	The METS technique builds on established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage.
29273221	4	1	theme	modular	531:537	arg1	fabrication					539:549	modular fabrication	531:549	modular fabrication	531:549	In this study, we present a method for developing large-scale engineered cartilage surfaces through modular fabrication.
29273221	10	2	theme	mechanical	1251:1260	arg1	properties					1262:1271	Compressive mechanical properties	1239:1271	Compressive mechanical properties	1239:1271	Compressive mechanical properties and glycosaminoglycan (GAG) content of METS and individual constructs increased significantly over time.
29273221	5	3	theme	large	695:699	arg1	scaffolds					701:709	large scaffolds	695:709	large scaffolds with nutrient channels	695:732	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	5	4	theme	under-utilized	627:640	arg1	properties					656:665	the well-known, but largely under-utilized self-adhesion properties	599:665	the well-known, but largely under-utilized self-adhesion properties of de novo tissue	599:683	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	6	5	theme	bonds	854:858	arg1	strength					838:845	the tensile mechanical strength	815:845	the tensile mechanical strength of the bonds between attached constructs	815:886	Compressive mechanical properties were evaluated throughout METS specimens, and the tensile mechanical strength of the bonds between attached constructs was evaluated over time.
29273221	10	6	theme	Compressive	1239:1249	arg1	properties					1262:1271	Compressive mechanical properties	1239:1271	Compressive mechanical properties	1239:1271	Compressive mechanical properties and glycosaminoglycan (GAG) content of METS and individual constructs increased significantly over time.
29273221	3	7	theme	engineered	379:388	arg1	tissues					390:396	large engineered tissues	373:396	large engineered tissues	373:396	However, current approaches in translating these techniques to developing large engineered tissues remains a significant challenge.
29273221	8	8	theme	METS	1109:1112	arg1	samples					1114:1120	the METS samples	1105:1120	the METS samples	1105:1120	Results showed that by Day 14, stable connections had formed between the constructs in the METS samples.
29273221	1	9	from	osteoarthritis	155:168	arg1	repair					119:124	repair	119:124	repair itself from focal defects or osteoarthritis	119:168	Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis.
29273221	12	10	theme	large-scale	1641:1651	arg1	cartilage					1664:1672	large-scale engineered cartilage	1641:1672	large-scale engineered cartilage	1641:1672	This study demonstrated that modular fabrication is a viable technique for creating large-scale engineered cartilage, which can be broadly applied to many tissue engineering applications and construct geometries.
29273221	4	11	theme	cartilage	504:512	arg1	surfaces					514:521	large-scale engineered cartilage surfaces	481:521	large-scale engineered cartilage surfaces	481:521	In this study, we present a method for developing large-scale engineered cartilage surfaces through modular fabrication.
29273221	9	12	theme	rigid	1169:1173	arg1	sheet					1175:1179	a rigid sheet	1167:1179	a rigid sheet	1167:1179	By Day 21, bonds were robust enough to form a rigid sheet and continued to increase in size and strength over time.
29273221	6	13	theme	attached	868:875	arg1	constructs					877:886	attached constructs	868:886	attached constructs	868:886	Compressive mechanical properties were evaluated throughout METS specimens, and the tensile mechanical strength of the bonds between attached constructs was evaluated over time.
29273221	10	14	theme	constructs	1332:1341	arg1	properties					1262:1271	Compressive mechanical properties	1239:1271	Compressive mechanical properties	1239:1271	Compressive mechanical properties and glycosaminoglycan (GAG) content of METS and individual constructs increased significantly over time.
29273221	10	14	theme	constructs	1332:1341	arg1	content					1301:1307	glycosaminoglycan (GAG) content	1277:1307	glycosaminoglycan (GAG) content	1277:1307	Compressive mechanical properties and glycosaminoglycan (GAG) content of METS and individual constructs increased significantly over time.
29273221	6	15	theme	mechanical	747:756	arg1	properties					758:767	Compressive mechanical properties	735:767	Compressive mechanical properties	735:767	Compressive mechanical properties were evaluated throughout METS specimens, and the tensile mechanical strength of the bonds between attached constructs was evaluated over time.
29273221	4	16	theme	engineered	493:502	arg1	surfaces					514:521	large-scale engineered cartilage surfaces	481:521	large-scale engineered cartilage surfaces	481:521	In this study, we present a method for developing large-scale engineered cartilage surfaces through modular fabrication.
29273221	5	17	theme	de	670:671	arg1	tissue					678:683	de novo tissue	670:683	de novo tissue	670:683	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	1	18	from	defects	144:150	arg1	repair					119:124	repair	119:124	repair itself from focal defects or osteoarthritis	119:168	Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis.
29273221	1	19	theme	Native	68:73	arg1	cartilage					85:93	Native articular cartilage	68:93	Native articular cartilage	68:93	Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis.
29273221	7	20	theme	biochemical	933:943	arg1	assays					945:950	biochemical assays	933:950	biochemical assays	933:950	Raman spectroscopy, biochemical assays, and histology were performed to investigate matrix distribution.
29273221	3	21	theme	large	373:377	arg1	tissues					390:396	large engineered tissues	373:396	large engineered tissues	373:396	However, current approaches in translating these techniques to developing large engineered tissues remains a significant challenge.
29273221	11	22	theme	established	1407:1417	arg1	accomplishments					1438:1452	established tissue engineering accomplishments	1407:1452	established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage	1407:1554	The METS technique builds on established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage.
29273221	5	23	theme	well-known	603:612	arg1	properties					656:665	the well-known, but largely under-utilized self-adhesion properties	599:665	the well-known, but largely under-utilized self-adhesion properties of de novo tissue	599:683	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	1	24	theme	articular	75:83	arg1	cartilage					85:93	Native articular cartilage	68:93	Native articular cartilage	68:93	Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis.
29273221	7	25	theme	Raman	913:917	arg1	spectroscopy					919:930	Raman spectroscopy	913:930	Raman spectroscopy	913:930	Raman spectroscopy, biochemical assays, and histology were performed to investigate matrix distribution.
29273221	0	26	theme	modular	2:8	arg1	approach					10:17	A modular approach	0:17	A modular approach to	0:20	A modular approach to creating large engineered cartilage surfaces.
29273221	12	27	theme	tissue	1712:1717	arg1	applications					1731:1742	many tissue engineering applications	1707:1742	many tissue engineering applications	1707:1742	This study demonstrated that modular fabrication is a viable technique for creating large-scale engineered cartilage, which can be broadly applied to many tissue engineering applications and construct geometries.
29273221	5	28	theme	tissue	678:683	arg1	properties					656:665	the well-known, but largely under-utilized self-adhesion properties	599:665	the well-known, but largely under-utilized self-adhesion properties of de novo tissue	599:683	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	5	29	theme	Modular	552:558	arg1	METS					588:591	Modular Engineered Tissue Surfaces (METS)	552:592	Modular Engineered Tissue Surfaces (METS)	552:592	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	5	30	dep	de	670:671	arg1	novo					673:676	novo	673:676	novo	673:676	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	12	31	theme	many	1707:1710	arg1	applications					1731:1742	many tissue engineering applications	1707:1742	many tissue engineering applications	1707:1742	This study demonstrated that modular fabrication is a viable technique for creating large-scale engineered cartilage, which can be broadly applied to many tissue engineering applications and construct geometries.
29273221	7	32	theme	matrix	997:1002	arg1	distribution					1004:1015	matrix distribution	997:1015	matrix distribution	997:1015	Raman spectroscopy, biochemical assays, and histology were performed to investigate matrix distribution.
29273221	6	33	theme	Compressive	735:745	arg1	properties					758:767	Compressive mechanical properties	735:767	Compressive mechanical properties	735:767	Compressive mechanical properties were evaluated throughout METS specimens, and the tensile mechanical strength of the bonds between attached constructs was evaluated over time.
29273221	10	34	theme	individual	1321:1330	arg1	constructs					1332:1341	individual constructs	1321:1341	individual constructs	1321:1341	Compressive mechanical properties and glycosaminoglycan (GAG) content of METS and individual constructs increased significantly over time.
29273221	8	35	theme	stable	1049:1054	arg1	connections					1056:1066	stable connections	1049:1066	stable connections	1049:1066	Results showed that by Day 14, stable connections had formed between the constructs in the METS samples.
29273221	1	36	theme	limited	99:105	arg1	capacity					107:114	limited capacity	99:114	limited capacity to repair itself from focal defects or osteoarthritis	99:168	Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis.
29273221	12	37	theme	engineering	1719:1729	arg1	applications					1731:1742	many tissue engineering applications	1707:1742	many tissue engineering applications	1707:1742	This study demonstrated that modular fabrication is a viable technique for creating large-scale engineered cartilage, which can be broadly applied to many tissue engineering applications and construct geometries.
29273221	11	38	theme	native	1539:1544	arg1	cartilage					1546:1554	native cartilage	1539:1554	native cartilage	1539:1554	The METS technique builds on established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage.
29273221	5	39	with	scaffolds	701:709	arg1	channels					725:732	nutrient channels	716:732	nutrient channels	716:732	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	0	40	theme	large	31:35	arg1	surfaces					58:65	large engineered cartilage surfaces	31:65	large engineered cartilage surfaces	31:65	A modular approach to creating large engineered cartilage surfaces.
29273221	8	41	from	constructs	1091:1100	arg1	samples					1114:1120	the METS samples	1105:1120	the METS samples	1105:1120	Results showed that by Day 14, stable connections had formed between the constructs in the METS samples.
29273221	11	42	with	constructs	1468:1477	arg1	properties					1516:1525	compressive properties	1504:1525	compressive properties approaching native cartilage	1504:1554	The METS technique builds on established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage.
29273221	11	42	with	constructs	1468:1477	arg1	composition					1488:1498	GAG composition	1484:1498	GAG composition	1484:1498	The METS technique builds on established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage.
29273221	12	43	theme	construct	1748:1756	arg1	geometries					1758:1767	construct geometries	1748:1767	construct geometries	1748:1767	This study demonstrated that modular fabrication is a viable technique for creating large-scale engineered cartilage, which can be broadly applied to many tissue engineering applications and construct geometries.
29273221	4	44	attach	present	449:455	arg2	we					446:447	we	446:447	we	446:447	In this study, we present a method for developing large-scale engineered cartilage surfaces through modular fabrication.
29273221	4	44	attach	present	449:455	arg1	study					439:443	this study	434:443	this study	434:443	In this study, we present a method for developing large-scale engineered cartilage surfaces through modular fabrication.
29273221	11	45	theme	tissue	1419:1424	arg1	accomplishments					1438:1452	established tissue engineering accomplishments	1407:1452	established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage	1407:1554	The METS technique builds on established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage.
29273221	2	46	theme	Tissue	171:176	arg1	engineering					178:188	Tissue engineering	171:188	Tissue engineering	171:188	Tissue engineering has provided a promising biological treatment strategy that is currently being evaluated in clinical trials.
29273221	6	47	theme	mechanical	827:836	arg1	strength					838:845	the tensile mechanical strength	815:845	the tensile mechanical strength of the bonds between attached constructs	815:886	Compressive mechanical properties were evaluated throughout METS specimens, and the tensile mechanical strength of the bonds between attached constructs was evaluated over time.
29273221	0	48	theme	cartilage	48:56	arg1	surfaces					58:65	large engineered cartilage surfaces	31:65	large engineered cartilage surfaces	31:65	A modular approach to creating large engineered cartilage surfaces.
29273221	11	49	theme	GAG	1484:1486	arg1	composition					1488:1498	GAG composition	1484:1498	GAG composition	1484:1498	The METS technique builds on established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage.
29273221	5	50	theme	nutrient	716:723	arg1	channels					725:732	nutrient channels	716:732	nutrient channels	716:732	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	6	51	theme	tensile	819:825	arg1	strength					838:845	the tensile mechanical strength	815:845	the tensile mechanical strength of the bonds between attached constructs	815:886	Compressive mechanical properties were evaluated throughout METS specimens, and the tensile mechanical strength of the bonds between attached constructs was evaluated over time.
29273221	12	52	theme	viable	1611:1616	arg1	technique					1618:1626	a viable technique	1609:1626	a viable technique	1609:1626	This study demonstrated that modular fabrication is a viable technique for creating large-scale engineered cartilage, which can be broadly applied to many tissue engineering applications and construct geometries.
29273221	12	52	theme	viable	1611:1616	arg1	fabrication					1594:1604	modular fabrication	1586:1604	modular fabrication	1586:1604	This study demonstrated that modular fabrication is a viable technique for creating large-scale engineered cartilage, which can be broadly applied to many tissue engineering applications and construct geometries.
29273221	0	53	theme	engineered	37:46	arg1	surfaces					58:65	large engineered cartilage surfaces	31:65	large engineered cartilage surfaces	31:65	A modular approach to creating large engineered cartilage surfaces.
29273221	1	54	contain	has	95:97	arg2	capacity					107:114	limited capacity	99:114	limited capacity to repair itself from focal defects or osteoarthritis	99:168	Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis.
29273221	1	54	contain	has	95:97	arg1	cartilage					85:93	Native articular cartilage	68:93	Native articular cartilage	68:93	Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis.
29273221	11	55	theme	compressive	1504:1514	arg1	properties					1516:1525	compressive properties	1504:1525	compressive properties approaching native cartilage	1504:1554	The METS technique builds on established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage.
29273221	2	56	theme	biological	215:224	arg1	strategy					236:243	a promising biological treatment strategy	203:243	a promising biological treatment strategy that is currently being evaluated in clinical trials	203:296	Tissue engineering has provided a promising biological treatment strategy that is currently being evaluated in clinical trials.
29273221	2	57	theme	treatment	226:234	arg1	strategy					236:243	a promising biological treatment strategy	203:243	a promising biological treatment strategy that is currently being evaluated in clinical trials	203:296	Tissue engineering has provided a promising biological treatment strategy that is currently being evaluated in clinical trials.
29273221	11	58	theme	METS	1382:1385	arg1	technique					1387:1395	The METS technique	1378:1395	The METS technique	1378:1395	The METS technique builds on established tissue engineering accomplishments of developing constructs with GAG composition and compressive properties approaching native cartilage.
29273221	4	59	theme	large-scale	481:491	arg1	surfaces					514:521	large-scale engineered cartilage surfaces	481:521	large-scale engineered cartilage surfaces	481:521	In this study, we present a method for developing large-scale engineered cartilage surfaces through modular fabrication.
29273221	2	60	theme	promising	205:213	arg1	strategy					236:243	a promising biological treatment strategy	203:243	a promising biological treatment strategy that is currently being evaluated in clinical trials	203:296	Tissue engineering has provided a promising biological treatment strategy that is currently being evaluated in clinical trials.
29273221	5	61	theme	self-adhesion	642:654	arg1	properties					656:665	the well-known, but largely under-utilized self-adhesion properties	599:665	the well-known, but largely under-utilized self-adhesion properties of de novo tissue	599:683	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	6	62	theme	METS	795:798	arg1	specimens					800:808	METS specimens	795:808	METS specimens	795:808	Compressive mechanical properties were evaluated throughout METS specimens, and the tensile mechanical strength of the bonds between attached constructs was evaluated over time.
29273221	3	63	theme	current	308:314	arg1	approaches					316:325	current approaches	308:325	current approaches in translating these techniques to developing large engineered tissues	308:396	However, current approaches in translating these techniques to developing large engineered tissues remains a significant challenge.
29273221	12	64	theme	modular	1586:1592	arg1	technique					1618:1626	a viable technique	1609:1626	a viable technique	1609:1626	This study demonstrated that modular fabrication is a viable technique for creating large-scale engineered cartilage, which can be broadly applied to many tissue engineering applications and construct geometries.
29273221	12	64	theme	modular	1586:1592	arg1	fabrication					1594:1604	modular fabrication	1586:1604	modular fabrication	1586:1604	This study demonstrated that modular fabrication is a viable technique for creating large-scale engineered cartilage, which can be broadly applied to many tissue engineering applications and construct geometries.
29273221	10	65	theme	METS	1312:1315	arg1	properties					1262:1271	Compressive mechanical properties	1239:1271	Compressive mechanical properties	1239:1271	Compressive mechanical properties and glycosaminoglycan (GAG) content of METS and individual constructs increased significantly over time.
29273221	10	65	theme	METS	1312:1315	arg1	content					1301:1307	glycosaminoglycan (GAG) content	1277:1307	glycosaminoglycan (GAG) content	1277:1307	Compressive mechanical properties and glycosaminoglycan (GAG) content of METS and individual constructs increased significantly over time.
29273221	5	66	theme	Engineered	560:569	arg1	METS					588:591	Modular Engineered Tissue Surfaces (METS)	552:592	Modular Engineered Tissue Surfaces (METS)	552:592	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	12	67	theme	engineered	1653:1662	arg1	cartilage					1664:1672	large-scale engineered cartilage	1641:1672	large-scale engineered cartilage	1641:1672	This study demonstrated that modular fabrication is a viable technique for creating large-scale engineered cartilage, which can be broadly applied to many tissue engineering applications and construct geometries.
29273221	5	68	theme	Tissue	571:576	arg1	METS					588:591	Modular Engineered Tissue Surfaces (METS)	552:592	Modular Engineered Tissue Surfaces (METS)	552:592	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	1	69	theme	focal	138:142	arg1	defects					144:150	focal defects	138:150	focal defects	138:150	Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis.
29273221	10	70	theme	glycosaminoglycan	1277:1293	arg1	content					1301:1307	glycosaminoglycan (GAG) content	1277:1307	glycosaminoglycan (GAG) content	1277:1307	Compressive mechanical properties and glycosaminoglycan (GAG) content of METS and individual constructs increased significantly over time.
29273221	2	71	theme	clinical	282:289	arg1	trials					291:296	clinical trials	282:296	clinical trials	282:296	Tissue engineering has provided a promising biological treatment strategy that is currently being evaluated in clinical trials.
29273221	5	72	theme	Surfaces	578:585	arg1	METS					588:591	Modular Engineered Tissue Surfaces (METS)	552:592	Modular Engineered Tissue Surfaces (METS)	552:592	Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels.
29273221	3	73	theme	significant	408:418	arg1	challenge					420:428	a significant challenge	406:428	a significant challenge	406:428	However, current approaches in translating these techniques to developing large engineered tissues remains a significant challenge.
27987659	0	0	theme	mechanical	72:81	arg1	properties					83:92	mechanical properties	72:92	mechanical properties	72:92	Optimization of crosslinked poly(vinyl alcohol) nanocomposite films for mechanical properties.
27987659	1	1	theme	rotatable	574:582	arg1	RSM-CCRD					592:599	RSM-CCRD	592:599	RSM-CCRD	592:599	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	1	theme	rotatable	574:582	arg1	design					584:589	a response surface methodology-central composite rotatable design	525:589	a response surface methodology-central composite rotatable design (RSM-CCRD)	525:600	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	5	2	theme	intermolecular	1162:1175	arg1	hydrogen					1177:1184	intramolecular and intermolecular hydrogen and ether crosslinkages	1143:1208	hydrogen	1177:1184	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	6	3	dep	films	1307:1311	arg1	crosslinked					1343:1353	crosslinked	1343:1353	crosslinked with boric acid	1343:1369	Results showed that PVA/BCNC nanocomposite films plasticized with glycerol and crosslinked with boric acid showed appropriate mechanical properties that made them suitable as a disposable packaging film.
27987659	6	3	dep	films	1307:1311	arg1	plasticized					1313:1323	plasticized	1313:1323	plasticized with glycerol	1313:1337	Results showed that PVA/BCNC nanocomposite films plasticized with glycerol and crosslinked with boric acid showed appropriate mechanical properties that made them suitable as a disposable packaging film.
27987659	3	4	theme	5.00	909:912	arg1	%					913:913	%	913:913	%	913:913	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	4	5	theme	experimental	1041:1052	arg1	data					1054:1057	experimental data	1041:1057	experimental data	1041:1057	The predicted values for optimum conditions were in good agreement with experimental data.
27987659	3	6	theme	maximum	788:794	arg1	UTS					796:798	the maximum UTS	784:798	the maximum UTS (72.84MPa)	784:809	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	3	6	theme	maximum	788:794	arg1	72.84MPa					801:808	72.84MPa	801:808	72.84MPa	801:808	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	3	7	theme	%	966:966	arg1	content					951:957	a boric acid content	938:957	a boric acid content of 1.96%	938:966	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	3	7	theme	%	966:966	arg1	concentration					920:932	5.00% BCNC concentration	909:932	5.00% BCNC concentration	909:932	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	3	7	theme	%	966:966	arg1	concentration					894:906	13.89% glycerol concentration	878:906	13.89% glycerol concentration	878:906	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	0	8	link	crosslinked	16:26	arg1	poly					28:31	crosslinked poly	16:31	crosslinked poly(vinyl alcohol)	16:46	Optimization of crosslinked poly(vinyl alcohol) nanocomposite films for mechanical properties.
27987659	0	8	link	crosslinked	16:26	arg1	alcohol					39:45	vinyl alcohol	33:45	vinyl alcohol	33:45	Optimization of crosslinked poly(vinyl alcohol) nanocomposite films for mechanical properties.
27987659	2	9	theme	regression	745:754	arg1	analysis					756:763	multiple linear regression analysis	729:763	multiple linear regression analysis	729:763	Second-order polynomial models with high R2 values ranging from 0.945 to 0.977 were developed for the studied responses using multiple linear regression analysis.
27987659	6	10	theme	mechanical	1390:1399	arg1	properties					1401:1410	appropriate mechanical properties	1378:1410	appropriate mechanical properties that made them suitable as a disposable packaging film	1378:1465	Results showed that PVA/BCNC nanocomposite films plasticized with glycerol and crosslinked with boric acid showed appropriate mechanical properties that made them suitable as a disposable packaging film.
27987659	5	11	theme	ether	1190:1194	arg1	crosslinkages					1196:1208	intramolecular and intermolecular hydrogen and ether crosslinkages	1143:1208	crosslinkages	1196:1208	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	2	12	with	models	627:632	arg1	values					647:652	high R2 values	639:652	high R2 values ranging from 0.945 to 0.977	639:680	Second-order polynomial models with high R2 values ranging from 0.945 to 0.977 were developed for the studied responses using multiple linear regression analysis.
27987659	5	13	theme	boric	1241:1245	arg1	acid					1247:1250	boric acid	1241:1250	boric acid	1241:1250	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	2	14	theme	Second-order	603:614	arg1	models					627:632	Second-order polynomial models	603:632	Second-order polynomial models with high R2 values ranging from 0.945 to 0.977	603:680	Second-order polynomial models with high R2 values ranging from 0.945 to 0.977 were developed for the studied responses using multiple linear regression analysis.
27987659	6	15	theme	boric	1360:1364	arg1	acid					1366:1369	boric acid	1360:1369	boric acid	1360:1369	Results showed that PVA/BCNC nanocomposite films plasticized with glycerol and crosslinked with boric acid showed appropriate mechanical properties that made them suitable as a disposable packaging film.
27987659	1	16	theme	ultimate	248:255	arg1	UTS					275:277	UTS	275:277	UTS	275:277	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	16	theme	ultimate	248:255	arg1	strength					265:272	ultimate tensile strength	248:272	ultimate tensile strength (UTS)	248:278	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	TYM					333:335	TYM	333:335	TYM	333:335	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	deformation					415:425	puncture deformation	406:425	puncture deformation (PD)	406:430	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	toughness					477:485	puncture toughness	468:485	puncture toughness	468:485	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	elongation					281:290	elongation	281:290	elongation at break (EAB)	281:305	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	PD					428:429	PD	428:429	PD	428:429	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	modulus					450:456	puncture Young's modulus	433:456	puncture Young's modulus (PYM)	433:462	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	strength					265:272	ultimate tensile strength	248:272	ultimate tensile strength (UTS)	248:278	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	PYM					459:461	PYM	459:461	PYM	459:461	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	UTS					275:277	UTS	275:277	UTS	275:277	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	toughness					347:355	tensile toughness	339:355	tensile toughness to break (TT)	339:369	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	UPS					400:402	UPS	400:402	UPS	400:402	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	modulus					324:330	tensile Young's modulus	308:330	tensile Young's modulus (TYM)	308:336	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	17	from	break	295:299	arg1	strength					390:397	ultimate puncture strength	372:397	ultimate puncture strength (UPS)	372:403	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	18	theme	tensile	308:314	arg1	TYM					333:335	TYM	333:335	TYM	333:335	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	18	theme	tensile	308:314	arg1	modulus					324:330	tensile Young's modulus	308:330	tensile Young's modulus (TYM)	308:336	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	3	19	theme	13.89	878:882	arg1	%					883:883	%	883:883	%	883:883	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	6	20	theme	packaging	1452:1460	arg1	film					1462:1465	a disposable packaging film	1439:1465	a disposable packaging film	1439:1465	Results showed that PVA/BCNC nanocomposite films plasticized with glycerol and crosslinked with boric acid showed appropriate mechanical properties that made them suitable as a disposable packaging film.
27987659	5	21	theme	Fourier-transform	1060:1076	arg1	FTIR					1101:1104	FTIR	1101:1104	FTIR	1101:1104	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	5	21	theme	Fourier-transform	1060:1076	arg1	spectroscopy					1087:1098	Fourier-transform infrared spectroscopy	1060:1098	Fourier-transform infrared spectroscopy (FTIR) analysis	1060:1114	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	2	22	theme	linear	738:743	arg1	analysis					756:763	multiple linear regression analysis	729:763	multiple linear regression analysis	729:763	Second-order polynomial models with high R2 values ranging from 0.945 to 0.977 were developed for the studied responses using multiple linear regression analysis.
27987659	1	23	theme	Young	316:320	arg1	TYM					333:335	TYM	333:335	TYM	333:335	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	23	theme	Young	316:320	arg1	modulus					324:330	tensile Young's modulus	308:330	tensile Young's modulus (TYM)	308:336	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	3	24	theme	%	883:883	arg1	concentration					894:906	13.89% glycerol concentration	878:906	13.89% glycerol concentration	878:906	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	4	25	theme	predicted	973:981	arg1	values					983:988	The predicted values	969:988	The predicted values for optimum conditions	969:1011	The predicted values for optimum conditions were in good agreement with experimental data.
27987659	5	26	theme	crosslinkages	1196:1208	arg1	formation					1130:1138	the formation	1126:1138	the formation of intramolecular and intermolecular hydrogen and ether crosslinkages	1126:1208	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	1	27	theme	puncture	433:440	arg1	modulus					450:456	puncture Young's modulus	433:456	puncture Young's modulus (PYM)	433:462	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	27	theme	puncture	433:440	arg1	PYM					459:461	PYM	459:461	PYM	459:461	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	6	28	theme	appropriate	1378:1388	arg1	properties					1401:1410	appropriate mechanical properties	1378:1410	appropriate mechanical properties that made them suitable as a disposable packaging film	1378:1465	Results showed that PVA/BCNC nanocomposite films plasticized with glycerol and crosslinked with boric acid showed appropriate mechanical properties that made them suitable as a disposable packaging film.
27987659	3	29	theme	boric	940:944	arg1	acid					946:949	a boric acid	938:949	a boric acid content of 1.96%	938:966	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	1	30	theme	ultimate	372:379	arg1	UPS					400:402	UPS	400:402	UPS	400:402	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	30	theme	ultimate	372:379	arg1	strength					390:397	ultimate puncture strength	372:397	ultimate puncture strength (UPS)	372:403	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	31	theme	Young	442:446	arg1	modulus					450:456	puncture Young's modulus	433:456	puncture Young's modulus (PYM)	433:462	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	31	theme	Young	442:446	arg1	PYM					459:461	PYM	459:461	PYM	459:461	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	32	theme	boric	163:167	arg1	concentrations					174:187	boric acid concentrations	163:187	boric acid concentrations	163:187	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	33	theme	tensile	257:263	arg1	UTS					275:277	UTS	275:277	UTS	275:277	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	33	theme	tensile	257:263	arg1	strength					265:272	ultimate tensile strength	248:272	ultimate tensile strength (UTS)	248:278	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	34	theme	puncture	381:388	arg1	UPS					400:402	UPS	400:402	UPS	400:402	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	34	theme	puncture	381:388	arg1	strength					390:397	ultimate puncture strength	372:397	ultimate puncture strength (UPS)	372:403	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	5	35	theme	spectroscopy	1087:1098	arg1	analysis					1107:1114	Fourier-transform infrared spectroscopy (FTIR) analysis	1060:1114	Fourier-transform infrared spectroscopy (FTIR) analysis	1060:1114	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	1	36	theme	acid	169:172	arg1	concentrations					174:187	boric acid concentrations	163:187	boric acid concentrations	163:187	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	0	37	theme	poly	28:31	arg1	Optimization					0:11	Optimization	0:11	Optimization of crosslinked poly(vinyl alcohol)	0:46	Optimization of crosslinked poly(vinyl alcohol) nanocomposite films for mechanical properties.
27987659	5	38	theme	BCNC	1224:1227	arg1	chains					1229:1234	BCNC chains	1224:1234	BCNC chains	1224:1234	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	1	39	theme	concentrations	174:187	arg1	effects					99:105	The effects	95:105	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT),	95:500	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	0	40	theme	crosslinked	16:26	arg1	poly					28:31	crosslinked poly	16:31	crosslinked poly(vinyl alcohol)	16:46	Optimization of crosslinked poly(vinyl alcohol) nanocomposite films for mechanical properties.
27987659	0	40	theme	crosslinked	16:26	arg1	alcohol					39:45	vinyl alcohol	33:45	vinyl alcohol	33:45	Optimization of crosslinked poly(vinyl alcohol) nanocomposite films for mechanical properties.
27987659	3	41	theme	acid	946:949	arg1	content					951:957	a boric acid content	938:957	a boric acid content of 1.96%	938:966	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	6	42	theme	disposable	1441:1450	arg1	film					1462:1465	a disposable packaging film	1439:1465	a disposable packaging film	1439:1465	Results showed that PVA/BCNC nanocomposite films plasticized with glycerol and crosslinked with boric acid showed appropriate mechanical properties that made them suitable as a disposable packaging film.
27987659	2	43	theme	R2	644:645	arg1	values					647:652	high R2 values	639:652	high R2 values ranging from 0.945 to 0.977	639:680	Second-order polynomial models with high R2 values ranging from 0.945 to 0.977 were developed for the studied responses using multiple linear regression analysis.
27987659	0	44	theme	vinyl	33:37	arg1	poly					28:31	crosslinked poly	16:31	crosslinked poly(vinyl alcohol)	16:46	Optimization of crosslinked poly(vinyl alcohol) nanocomposite films for mechanical properties.
27987659	0	44	theme	vinyl	33:37	arg1	alcohol					39:45	vinyl alcohol	33:45	vinyl alcohol	33:45	Optimization of crosslinked poly(vinyl alcohol) nanocomposite films for mechanical properties.
27987659	3	45	theme	glycerol	885:892	arg1	concentration					894:906	13.89% glycerol concentration	878:906	13.89% glycerol concentration	878:906	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	2	46	theme	high	639:642	arg1	values					647:652	high R2 values	639:652	high R2 values ranging from 0.945 to 0.977	639:680	Second-order polynomial models with high R2 values ranging from 0.945 to 0.977 were developed for the studied responses using multiple linear regression analysis.
27987659	4	47	with	agreement	1026:1034	arg1	data					1054:1057	experimental data	1041:1057	experimental data	1041:1057	The predicted values for optimum conditions were in good agreement with experimental data.
27987659	5	48	theme	hydrogen	1177:1184	arg1	formation					1130:1138	the formation	1126:1138	the formation of intramolecular and intermolecular hydrogen and ether crosslinkages	1126:1208	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	1	49	theme	mechanical	196:205	arg1	properties					207:216	the mechanical properties	192:216	the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT),	192:500	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	50	theme	response	527:534	arg1	RSM-CCRD					592:599	RSM-CCRD	592:599	RSM-CCRD	592:599	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	50	theme	response	527:534	arg1	design					584:589	a response surface methodology-central composite rotatable design	525:589	a response surface methodology-central composite rotatable design (RSM-CCRD)	525:600	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	51	theme	surface	536:542	arg1	RSM-CCRD					592:599	RSM-CCRD	592:599	RSM-CCRD	592:599	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	51	theme	surface	536:542	arg1	design					584:589	a response surface methodology-central composite rotatable design	525:589	a response surface methodology-central composite rotatable design (RSM-CCRD)	525:600	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	4	52	theme	good	1021:1024	arg1	agreement					1026:1034	good agreement	1021:1034	good agreement with experimental data	1021:1057	The predicted values for optimum conditions were in good agreement with experimental data.
27987659	3	53	dep	showed	777:782	arg1	achieved					866:873	achieved	866:873	showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%	777:966	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	1	54	theme	glycerol	110:117	arg1	effects					99:105	The effects	95:105	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT),	95:500	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	6	55	theme	nanocomposite	1293:1305	arg1	films					1307:1311	PVA/BCNC nanocomposite films	1284:1311	PVA/BCNC nanocomposite films plasticized with glycerol and crosslinked with boric acid	1284:1369	Results showed that PVA/BCNC nanocomposite films plasticized with glycerol and crosslinked with boric acid showed appropriate mechanical properties that made them suitable as a disposable packaging film.
27987659	3	56	theme	%	913:913	arg1	concentration					920:932	5.00% BCNC concentration	909:932	5.00% BCNC concentration	909:932	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	1	57	theme	tensile	339:345	arg1	toughness					347:355	tensile toughness	339:355	tensile toughness to break (TT)	339:369	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	6	58	theme	PVA/BCNC	1284:1291	arg1	films					1307:1311	PVA/BCNC nanocomposite films	1284:1311	PVA/BCNC nanocomposite films plasticized with glycerol and crosslinked with boric acid	1284:1369	Results showed that PVA/BCNC nanocomposite films plasticized with glycerol and crosslinked with boric acid showed appropriate mechanical properties that made them suitable as a disposable packaging film.
27987659	4	59	theme	optimum	994:1000	arg1	conditions					1002:1011	optimum conditions	994:1011	optimum conditions	994:1011	The predicted values for optimum conditions were in good agreement with experimental data.
27987659	1	60	from	effects	99:105	arg1	properties					207:216	the mechanical properties	192:216	the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT),	192:500	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	2	61	theme	multiple	729:736	arg1	analysis					756:763	multiple linear regression analysis	729:763	multiple linear regression analysis	729:763	Second-order polynomial models with high R2 values ranging from 0.945 to 0.977 were developed for the studied responses using multiple linear regression analysis.
27987659	3	62	theme	BCNC	915:918	arg1	concentration					920:932	5.00% BCNC concentration	909:932	5.00% BCNC concentration	909:932	The models showed the maximum UTS (72.84MPa), EAB (293.43%), UPS (4.64MPa) and PD (31.80%) could be achieved at 13.89% glycerol concentration, 5.00% BCNC concentration and a boric acid content of 1.96%.
27987659	1	63	dep	films	231:235	arg1	including					238:246	including	238:246	including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT)	238:499	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	64	theme	puncture	406:413	arg1	deformation					415:425	puncture deformation	406:425	puncture deformation (PD)	406:430	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	64	theme	puncture	406:413	arg1	PD					428:429	PD	428:429	PD	428:429	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	65	theme	bacterial	120:128	arg1	nanocrystal					140:150	bacterial cellulose nanocrystal	120:150	bacterial cellulose nanocrystal (BCNC)	120:157	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	65	theme	bacterial	120:128	arg1	BCNC					153:156	BCNC	153:156	BCNC	153:156	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	66	theme	puncture	468:475	arg1	toughness					477:485	puncture toughness	468:485	puncture toughness	468:485	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	2	67	theme	polynomial	616:625	arg1	models					627:632	Second-order polynomial models	603:632	Second-order polynomial models with high R2 values ranging from 0.945 to 0.977	603:680	Second-order polynomial models with high R2 values ranging from 0.945 to 0.977 were developed for the studied responses using multiple linear regression analysis.
27987659	1	68	theme	methodology-central	544:562	arg1	RSM-CCRD					592:599	RSM-CCRD	592:599	RSM-CCRD	592:599	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	68	theme	methodology-central	544:562	arg1	design					584:589	a response surface methodology-central composite rotatable design	525:589	a response surface methodology-central composite rotatable design (RSM-CCRD)	525:600	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	5	69	theme	intramolecular	1143:1156	arg1	hydrogen					1177:1184	intramolecular and intermolecular hydrogen and ether crosslinkages	1143:1208	hydrogen	1177:1184	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	1	70	theme	cellulose	130:138	arg1	nanocrystal					140:150	bacterial cellulose nanocrystal	120:150	bacterial cellulose nanocrystal (BCNC)	120:157	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	70	theme	cellulose	130:138	arg1	BCNC					153:156	BCNC	153:156	BCNC	153:156	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	5	71	theme	infrared	1078:1085	arg1	FTIR					1101:1104	FTIR	1101:1104	FTIR	1101:1104	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	5	71	theme	infrared	1078:1085	arg1	spectroscopy					1087:1098	Fourier-transform infrared spectroscopy	1060:1098	Fourier-transform infrared spectroscopy (FTIR) analysis	1060:1114	Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the formation of intramolecular and intermolecular hydrogen and ether crosslinkages in PVA and/or BCNC chains when boric acid is applied.
27987659	1	72	theme	PVA	221:223	arg1	properties					207:216	the mechanical properties	192:216	the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT),	192:500	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	73	theme	composite	564:572	arg1	RSM-CCRD					592:599	RSM-CCRD	592:599	RSM-CCRD	592:599	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	73	theme	composite	564:572	arg1	design					584:589	a response surface methodology-central composite rotatable design	525:589	a response surface methodology-central composite rotatable design (RSM-CCRD)	525:600	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	1	74	theme	nanocrystal	140:150	arg1	effects					99:105	The effects	95:105	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT),	95:500	The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD).
27987659	2	75	theme	studied	705:711	arg1	responses					713:721	the studied responses	701:721	the studied responses using multiple linear regression analysis	701:763	Second-order polynomial models with high R2 values ranging from 0.945 to 0.977 were developed for the studied responses using multiple linear regression analysis.
24342180	6	0	theme	lipid	883:887	arg1	content					889:895	its total lipid content	873:895	its total lipid content	873:895	The optimum corresponded to 0.593 g of carrageenans/100 g and 0.320 g of milk proteins and its total lipid content was 6.3 g/100 g.
24342180	0	1	theme	proteins	58:65	arg1	optimization					37:48	optimization	37:48	optimization of milk proteins and carrageenan contents using response surface methodology	37:125	Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology.
24342180	7	2	theme	experimental	1005:1016	arg1	responses					1018:1026	experimental responses	1005:1026	experimental responses	1005:1026	This formulation was prepared and evaluated showing a good agreement between predicted and experimental responses.
24342180	1	3	theme	cooking	233:239	arg1	CL					247:248	CL	247:248	CL	247:248	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	1	3	theme	cooking	233:239	arg1	loss					241:244	cooking loss	233:244	cooking loss (CL)	233:249	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	0	4	theme	contents	83:90	arg1	optimization					37:48	optimization	37:48	optimization of milk proteins and carrageenan contents using response surface methodology	37:125	Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology.
24342180	1	5	theme	texture	292:298	arg1	attributes					300:309	texture attributes	292:309	texture attributes	292:309	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	8	6	with	sausages	1072:1079	arg1	quality					1132:1138	good nutritional quality	1115:1138	good nutritional quality	1115:1138	These additives could produce low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics than traditional ones.
24342180	8	6	with	sausages	1072:1079	arg1	characteristics					1152:1166	similar characteristics	1144:1166	similar characteristics	1144:1166	These additives could produce low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics than traditional ones.
24342180	8	6	with	sausages	1072:1079	arg1	oil					1106:1108	pre-emulsified fish oil	1086:1108	pre-emulsified fish oil	1086:1108	These additives could produce low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics than traditional ones.
24342180	1	7	used	used	161:164	arg2	methodology					145:155	Response surface methodology	128:155	Response surface methodology	128:155	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	0	8	theme	carrageenan	71:81	arg1	contents					83:90	carrageenan contents	71:90	carrageenan contents	71:90	Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology.
24342180	6	9	theme	g	838:838	arg1	g					816:816	0.593 g	810:816	0.593 g of carrageenans/100 g and 0.320 g of milk proteins	810:867	The optimum corresponded to 0.593 g of carrageenans/100 g and 0.320 g of milk proteins and its total lipid content was 6.3 g/100 g.
24342180	3	10	theme	milk	506:509	arg1	proteins					511:518	milk proteins	506:518	milk proteins	506:518	Changes in carrageenans affected more the responses than milk proteins levels.
24342180	0	11	theme	response	98:105	arg1	methodology					115:125	response surface methodology	98:125	response surface methodology	98:125	Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology.
24342180	6	12	theme	g	850:850	arg1	g					816:816	0.593 g	810:816	0.593 g of carrageenans/100 g and 0.320 g of milk proteins	810:867	The optimum corresponded to 0.593 g of carrageenans/100 g and 0.320 g of milk proteins and its total lipid content was 6.3 g/100 g.
24342180	2	13	theme	objective	428:436	arg1	responses					438:446	the objective responses	424:446	the objective responses	424:446	A central-composite design was used to develop models for the objective responses.
24342180	1	14	theme	low-fat	314:320	arg1	sausages					327:334	low-fat meat sausages	314:334	low-fat meat sausages with pre-emulsified fish oil	314:363	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	8	15	theme	good	1115:1118	arg1	quality					1132:1138	good nutritional quality	1115:1138	good nutritional quality	1115:1138	These additives could produce low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics than traditional ones.
24342180	0	16	theme	meat	8:11	arg1	sausages					13:20	Low-fat meat sausages	0:20	Low-fat meat sausages with fish oil	0:34	Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology.
24342180	1	17	theme	meat	322:325	arg1	sausages					327:334	low-fat meat sausages	314:334	low-fat meat sausages with pre-emulsified fish oil	314:363	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	8	18	theme	meat	1067:1070	arg1	sausages					1072:1079	low-fat meat sausages	1059:1079	low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics	1059:1166	These additives could produce low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics than traditional ones.
24342180	0	19	theme	Low-fat	0:6	arg1	sausages					13:20	Low-fat meat sausages	0:20	Low-fat meat sausages with fish oil	0:34	Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology.
24342180	5	20	dep	products	747:754	arg1	g					760:760	20 g	757:760	20 g of pork fat/100 g	757:778	Responses were optimized simultaneously minimizing CL and WLC; ranges for hardness and springiness corresponded to commercial products (20 g of pork fat/100 g).
24342180	1	21	dep	used	161:164	arg1	ι-carrageenans					215:228	ι-carrageenans	215:228	ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil	215:363	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	4	22	theme	product	612:618	arg1	hardness					579:586	hardness	579:586	hardness	579:586	Convenience functions were calculated for CL, WLC, hardness, and springiness of the product.
24342180	4	22	theme	product	612:618	arg1	springiness					593:603	springiness	593:603	springiness	593:603	Convenience functions were calculated for CL, WLC, hardness, and springiness of the product.
24342180	4	22	theme	product	612:618	arg1	WLC					574:576	WLC	574:576	WLC	574:576	Convenience functions were calculated for CL, WLC, hardness, and springiness of the product.
24342180	4	22	theme	product	612:618	arg1	CL					570:571	CL	570:571	CL	570:571	Convenience functions were calculated for CL, WLC, hardness, and springiness of the product.
24342180	5	23	theme	pork	765:768	arg1	g					778:778	pork fat/100 g	765:778	pork fat/100 g	765:778	Responses were optimized simultaneously minimizing CL and WLC; ranges for hardness and springiness corresponded to commercial products (20 g of pork fat/100 g).
24342180	3	24	from	Changes	449:455	arg1	carrageenans					460:471	carrageenans	460:471	carrageenans	460:471	Changes in carrageenans affected more the responses than milk proteins levels.
24342180	2	25	used	used	397:400	arg2	design					386:391	A central-composite design	366:391	A central-composite design	366:391	A central-composite design was used to develop models for the objective responses.
24342180	2	26	theme	central-composite	368:384	arg1	design					386:391	A central-composite design	366:391	A central-composite design	366:391	A central-composite design was used to develop models for the objective responses.
24342180	4	27	theme	Convenience	528:538	arg1	functions					540:548	Convenience functions	528:548	Convenience functions	528:548	Convenience functions were calculated for CL, WLC, hardness, and springiness of the product.
24342180	0	28	theme	surface	107:113	arg1	methodology					115:125	response surface methodology	98:125	response surface methodology	98:125	Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology.
24342180	1	29	theme	sausages	327:334	arg1	ι-carrageenans					215:228	ι-carrageenans	215:228	ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil	215:363	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	3	30	dep	more	482:485	arg1	responses					491:499	the responses	487:499	the responses	487:499	Changes in carrageenans affected more the responses than milk proteins levels.
24342180	1	31	with	sausages	327:334	arg1	oil					361:363	pre-emulsified fish oil	341:363	pre-emulsified fish oil	341:363	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	1	32	theme	milk	191:194	arg1	proteins					196:203	milk proteins	191:203	milk proteins	191:203	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	5	33	theme	commercial	736:745	arg1	products					747:754	commercial products	736:754	commercial products (20 g of pork fat/100 g)	736:779	Responses were optimized simultaneously minimizing CL and WLC; ranges for hardness and springiness corresponded to commercial products (20 g of pork fat/100 g).
24342180	6	34	theme	total	877:881	arg1	content					889:895	its total lipid content	873:895	its total lipid content	873:895	The optimum corresponded to 0.593 g of carrageenans/100 g and 0.320 g of milk proteins and its total lipid content was 6.3 g/100 g.
24342180	7	35	theme	good	968:971	arg1	agreement					973:981	a good agreement	966:981	a good agreement between predicted and experimental responses	966:1026	This formulation was prepared and evaluated showing a good agreement between predicted and experimental responses.
24342180	0	36	dep	optimization	37:48	arg1	sausages					13:20	Low-fat meat sausages	0:20	Low-fat meat sausages with fish oil	0:34	Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology.
24342180	1	37	theme	proteins	196:203	arg1	effect					181:186	the effect	177:186	the effect of milk proteins and 2:1 κ	177:213	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	0	38	theme	fish	27:30	arg1	oil					32:34	fish oil	27:34	fish oil	27:34	Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology.
24342180	8	39	theme	similar	1144:1150	arg1	characteristics					1152:1166	similar characteristics	1144:1166	similar characteristics	1144:1166	These additives could produce low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics than traditional ones.
24342180	1	40	theme	pre-emulsified	341:354	arg1	oil					361:363	pre-emulsified fish oil	341:363	pre-emulsified fish oil	341:363	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	8	41	theme	nutritional	1120:1130	arg1	quality					1132:1138	good nutritional quality	1115:1138	good nutritional quality	1115:1138	These additives could produce low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics than traditional ones.
24342180	8	42	theme	traditional	1173:1183	arg1	ones					1185:1188	traditional ones	1173:1188	traditional ones	1173:1188	These additives could produce low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics than traditional ones.
24342180	8	43	theme	fish	1101:1104	arg1	oil					1106:1108	pre-emulsified fish oil	1086:1108	pre-emulsified fish oil	1086:1108	These additives could produce low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics than traditional ones.
24342180	1	44	theme	fish	356:359	arg1	oil					361:363	pre-emulsified fish oil	341:363	pre-emulsified fish oil	341:363	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	0	45	with	sausages	13:20	arg1	oil					32:34	fish oil	27:34	fish oil	27:34	Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology.
24342180	6	46	theme	proteins	860:867	arg1	g					838:838	carrageenans/100 g	821:838	carrageenans/100 g	821:838	The optimum corresponded to 0.593 g of carrageenans/100 g and 0.320 g of milk proteins and its total lipid content was 6.3 g/100 g.
24342180	6	46	theme	proteins	860:867	arg1	g					850:850	0.320 g	844:850	0.320 g	844:850	The optimum corresponded to 0.593 g of carrageenans/100 g and 0.320 g of milk proteins and its total lipid content was 6.3 g/100 g.
24342180	1	47	from	ι-carrageenans	215:228	arg1	CL					247:248	CL	247:248	CL	247:248	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	1	47	from	ι-carrageenans	215:228	arg1	weight					252:257	weight	252:257	weight lost by centrifugation (WLC)	252:286	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	1	47	from	ι-carrageenans	215:228	arg1	loss					241:244	cooking loss	233:244	cooking loss (CL)	233:249	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	1	47	from	ι-carrageenans	215:228	arg1	attributes					300:309	texture attributes	292:309	texture attributes	292:309	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	6	48	theme	milk	855:858	arg1	proteins					860:867	milk proteins	855:867	milk proteins	855:867	The optimum corresponded to 0.593 g of carrageenans/100 g and 0.320 g of milk proteins and its total lipid content was 6.3 g/100 g.
24342180	1	49	theme	2:1	209:211	arg1	κ					213:213	2:1 κ	209:213	2:1 κ	209:213	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	1	50	theme	Response	128:135	arg1	methodology					145:155	Response surface methodology	128:155	Response surface methodology	128:155	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	1	51	theme	κ	213:213	arg1	effect					181:186	the effect	177:186	the effect of milk proteins and 2:1 κ	177:213	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	0	52	theme	milk	53:56	arg1	proteins					58:65	milk proteins	53:65	milk proteins	53:65	Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology.
24342180	5	53	theme	g	778:778	arg1	g					760:760	20 g	757:760	20 g of pork fat/100 g	757:778	Responses were optimized simultaneously minimizing CL and WLC; ranges for hardness and springiness corresponded to commercial products (20 g of pork fat/100 g).
24342180	1	54	theme	surface	137:143	arg1	methodology					145:155	Response surface methodology	128:155	Response surface methodology	128:155	Response surface methodology was used to analyze the effect of milk proteins and 2:1 κ:ι-carrageenans on cooking loss (CL), weight lost by centrifugation (WLC) and texture attributes of low-fat meat sausages with pre-emulsified fish oil.
24342180	8	55	theme	low-fat	1059:1065	arg1	sausages					1072:1079	low-fat meat sausages	1059:1079	low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics	1059:1166	These additives could produce low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics than traditional ones.
24342180	3	56	theme	more	482:485	arg1	levels					520:525	more the responses than milk proteins levels	482:525	more the responses than milk proteins levels	482:525	Changes in carrageenans affected more the responses than milk proteins levels.
24342180	8	57	theme	pre-emulsified	1086:1099	arg1	oil					1106:1108	pre-emulsified fish oil	1086:1108	pre-emulsified fish oil	1086:1108	These additives could produce low-fat meat sausages with pre-emulsified fish oil with good nutritional quality and similar characteristics than traditional ones.
28423949	5	0	theme	38 ± 1.43	735:743	arg1	%					744:744	%	744:744	%	744:744	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	4	1	theme	ionic	459:463	arg1	method					474:479	ionic gelation method	459:479	ionic gelation method	459:479	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
28423949	5	2	theme	drug	634:637	arg1	1.25:0.79					655:663	1.25:0.79	655:663	1.25:0.79	655:663	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	5	2	theme	drug	634:637	arg1	ratio					648:652	drug: polymer ratio	634:652	drug: polymer ratio (1.25:0.79)	634:664	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	7	3	dep	permeability	895:906	arg1	times					881:885	times	881:885	times	881:885	It gave 4.76 times greater permeability than plain drug solution through porcine nasal mucosa.
28423949	5	4	contain	containing	623:632	arg1	NPs					619:621	NPs	619:621	NPs containing drug: polymer ratio (1.25:0.79)	619:664	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	5	4	contain	containing	623:632	arg2	ratio					648:652	drug: polymer ratio	634:652	drug: polymer ratio (1.25:0.79)	634:664	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	5	4	contain	containing	623:632	arg2	1.25:0.79					655:663	1.25:0.79	655:663	1.25:0.79	655:663	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	5	5	theme	%	744:744	arg1	efficiency					757:766	38 ± 1.43% entrapment efficiency	735:766	38 ± 1.43% entrapment efficiency	735:766	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	3	6	theme	CNS	326:328	arg1	uptake					330:335	the CNS uptake	322:335	the CNS uptake of efavirenz (EFV)	322:354	Hence, the present study is attempted to enhance the CNS uptake of efavirenz (EFV) by designing intranasal EFV nanoparticles (EFV-NPs).
28423949	8	7	theme	CNS	972:974	arg1	bioavailability					976:990	Enhanced CNS bioavailability	963:990	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs	963:1143	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	5	8	theme	polymer	640:646	arg1	1.25:0.79					655:663	1.25:0.79	655:663	1.25:0.79	655:663	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	5	8	theme	polymer	640:646	arg1	ratio					648:652	drug: polymer ratio	634:652	drug: polymer ratio (1.25:0.79)	634:664	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	1	9	from	CNS	156:158	arg1	Incompetence					78:89	Incompetence	78:89	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS	78:158	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	1	9	from	CNS	156:158	arg1	eradication					128:138	complete eradication	119:138	complete eradication of HIV from the CNS	119:158	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	1	9	from	CNS	156:158	arg1	issue					175:179	the biggest issue	163:179	the biggest issue in neuro-AIDS treatment	163:203	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	8	10	theme	solution	1016:1023	arg1	12.40-fold					993:1002	12.40-fold	993:1002	12.40-fold that of i.v solution	993:1023	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	5	11	theme	23.28 ± 1.5	705:715	arg1	%					716:716	%	716:716	%	716:716	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	6	12	theme	sustained	780:788	arg1	release					795:801	sustained drug release	780:801	sustained drug release (99.03 ± 0.30% in 8 h)	780:824	NPs showed sustained drug release (99.03 ± 0.30% in 8 h) and followed Fickian diffusion mechanism.
28423949	2	13	theme	ARV	268:270	arg1	penetration					253:263	the poor penetration	244:263	the poor penetration of ARV	244:270	The ineffectiveness is largely due to the poor penetration of ARV.
28423949	8	14	dep	12.40-fold	993:1002	arg1	that					1004:1007	that	1004:1007	that	1004:1007	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	4	15	theme	two-factor	556:565	arg1	design					611:616	two-factor, five-level circumscribed central composite design	556:616	two-factor, five-level circumscribed central composite design	556:616	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
28423949	5	16	theme	%	716:716	arg1	loading					723:729	23.28 ± 1.5% drug loading	705:729	23.28 ± 1.5% drug loading	705:729	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	10	17	with	histocompatible	1265:1279	arg1	mucosa					1300:1305	porcine nasal mucosa	1286:1305	porcine nasal mucosa	1286:1305	NPs appeared histocompatible with porcine nasal mucosa and non-toxic to L929 cell line.
28423949	7	18	dep	times	881:885	arg1	greater					887:893	greater	887:893	greater	887:893	It gave 4.76 times greater permeability than plain drug solution through porcine nasal mucosa.
28423949	1	19	theme	biggest	167:173	arg1	Incompetence					78:89	Incompetence	78:89	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS	78:158	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	1	19	theme	biggest	167:173	arg1	issue					175:179	the biggest issue	163:179	the biggest issue in neuro-AIDS treatment	163:203	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	5	20	theme	drug	718:721	arg1	loading					723:729	23.28 ± 1.5% drug loading	705:729	23.28 ± 1.5% drug loading	705:729	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	6	21	theme	diffusion	847:855	arg1	mechanism					857:865	followed Fickian diffusion mechanism	830:865	followed Fickian diffusion mechanism	830:865	NPs showed sustained drug release (99.03 ± 0.30% in 8 h) and followed Fickian diffusion mechanism.
28423949	8	22	theme	NPs	1141:1143	arg1	percentage					1054:1063	high drug-targeting percentage	1034:1063	high drug-targeting percentage (99.24%)	1034:1072	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	8	22	theme	NPs	1141:1143	arg1	administration					1123:1136	drug-targeting index (141.3) post-intranasal administration	1078:1136	drug-targeting index (141.3) post-intranasal administration of NPs	1078:1143	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	8	22	theme	NPs	1141:1143	arg1	EFV					1029:1031	EFV	1029:1031	EFV	1029:1031	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	8	22	theme	NPs	1141:1143	arg1	%					1071:1071	99.24%	1066:1071	99.24%	1066:1071	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	8	23	dep	bioavailability	976:990	arg1	12.40-fold					993:1002	12.40-fold	993:1002	12.40-fold that of i.v solution	993:1023	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	7	24	theme	porcine	941:947	arg1	mucosa					955:960	porcine nasal mucosa	941:960	porcine nasal mucosa	941:960	It gave 4.76 times greater permeability than plain drug solution through porcine nasal mucosa.
28423949	7	25	theme	drug	919:922	arg1	solution					924:931	plain drug solution	913:931	plain drug solution	913:931	It gave 4.76 times greater permeability than plain drug solution through porcine nasal mucosa.
28423949	6	26	theme	Fickian	839:845	arg1	mechanism					857:865	followed Fickian diffusion mechanism	830:865	followed Fickian diffusion mechanism	830:865	NPs showed sustained drug release (99.03 ± 0.30% in 8 h) and followed Fickian diffusion mechanism.
28423949	11	27	theme	mucoadhesive	1412:1423	arg1	EFV-NPs					1425:1431	intranasal mucoadhesive EFV-NPs	1401:1431	intranasal mucoadhesive EFV-NPs for the CNS targeting	1401:1453	Thus, CS-g-HPβCD served as a potential carrier in developing intranasal mucoadhesive EFV-NPs for the CNS targeting.
28423949	7	28	theme	plain	913:917	arg1	solution					924:931	plain drug solution	913:931	plain drug solution	913:931	It gave 4.76 times greater permeability than plain drug solution through porcine nasal mucosa.
28423949	1	29	theme	antiretrovirals	94:108	arg1	Incompetence					78:89	Incompetence	78:89	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS	78:158	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	1	29	theme	antiretrovirals	94:108	arg1	issue					175:179	the biggest issue	163:179	the biggest issue in neuro-AIDS treatment	163:203	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	3	30	theme	EFV	380:382	arg1	EFV-NPs					399:405	EFV-NPs	399:405	EFV-NPs	399:405	Hence, the present study is attempted to enhance the CNS uptake of efavirenz (EFV) by designing intranasal EFV nanoparticles (EFV-NPs).
28423949	3	30	theme	EFV	380:382	arg1	nanoparticles					384:396	intranasal EFV nanoparticles	369:396	intranasal EFV nanoparticles (EFV-NPs)	369:406	Hence, the present study is attempted to enhance the CNS uptake of efavirenz (EFV) by designing intranasal EFV nanoparticles (EFV-NPs).
28423949	0	31	theme	Intranasal	0:9	arg1	chitosan-g-HPβCD					11:26	Intranasal chitosan-g-HPβCD	0:26	Intranasal chitosan-g-HPβCD	0:26	Intranasal chitosan-g-HPβCD nanoparticles of efavirenz for the CNS targeting.
28423949	8	32	theme	EFV	1029:1031	arg1	bioavailability					976:990	Enhanced CNS bioavailability	963:990	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs	963:1143	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	10	33	theme	cell	1329:1332	arg1	line					1334:1337	L929 cell line	1324:1337	L929 cell line	1324:1337	NPs appeared histocompatible with porcine nasal mucosa and non-toxic to L929 cell line.
28423949	9	34	theme	CNS	1240:1242	arg1	uptake					1244:1249	high CNS uptake	1235:1249	high CNS uptake	1235:1249	These results are corroborated by gamma scintigraphy images, which revealed high CNS uptake.
28423949	10	35	theme	porcine	1286:1292	arg1	mucosa					1300:1305	porcine nasal mucosa	1286:1305	porcine nasal mucosa	1286:1305	NPs appeared histocompatible with porcine nasal mucosa and non-toxic to L929 cell line.
28423949	9	36	theme	gamma	1193:1197	arg1	images					1212:1217	gamma scintigraphy images	1193:1217	gamma scintigraphy images	1193:1217	These results are corroborated by gamma scintigraphy images, which revealed high CNS uptake.
28423949	4	37	theme	surface	520:526	arg1	methodology					528:538	quadratic response surface methodology	501:538	quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design	501:616	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
28423949	4	37	theme	surface	520:526	arg1	RSM					541:543	RSM	541:543	RSM	541:543	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
28423949	8	38	theme	drug-targeting	1039:1052	arg1	percentage					1054:1063	high drug-targeting percentage	1034:1063	high drug-targeting percentage (99.24%)	1034:1072	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	8	38	theme	drug-targeting	1039:1052	arg1	%					1071:1071	99.24%	1066:1071	99.24%	1066:1071	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	3	39	theme	efavirenz	340:348	arg1	uptake					330:335	the CNS uptake	322:335	the CNS uptake of efavirenz (EFV)	322:354	Hence, the present study is attempted to enhance the CNS uptake of efavirenz (EFV) by designing intranasal EFV nanoparticles (EFV-NPs).
28423949	8	40	theme	high	1034:1037	arg1	percentage					1054:1063	high drug-targeting percentage	1034:1063	high drug-targeting percentage (99.24%)	1034:1072	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	8	40	theme	high	1034:1037	arg1	%					1071:1071	99.24%	1066:1071	99.24%	1066:1071	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	6	41	theme	followed	830:837	arg1	mechanism					857:865	followed Fickian diffusion mechanism	830:865	followed Fickian diffusion mechanism	830:865	NPs showed sustained drug release (99.03 ± 0.30% in 8 h) and followed Fickian diffusion mechanism.
28423949	6	42	dep	release	795:801	arg1	%					816:816	99.03 ± 0.30%	804:816	99.03 ± 0.30% in 8 h	804:823	NPs showed sustained drug release (99.03 ± 0.30% in 8 h) and followed Fickian diffusion mechanism.
28423949	1	43	theme	neuro-AIDS	184:193	arg1	treatment					195:203	neuro-AIDS treatment	184:203	neuro-AIDS treatment	184:203	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	5	44	theme	entrapment	746:755	arg1	efficiency					757:766	38 ± 1.43% entrapment efficiency	735:766	38 ± 1.43% entrapment efficiency	735:766	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	11	45	theme	intranasal	1401:1410	arg1	EFV-NPs					1425:1431	intranasal mucoadhesive EFV-NPs	1401:1431	intranasal mucoadhesive EFV-NPs for the CNS targeting	1401:1453	Thus, CS-g-HPβCD served as a potential carrier in developing intranasal mucoadhesive EFV-NPs for the CNS targeting.
28423949	5	46	theme	198 ± 4.4 nm	686:697	arg1	size					699:702	198 ± 4.4 nm size	686:702	198 ± 4.4 nm size	686:702	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	4	47	dep	two-factor	556:565	arg1	circumscribed					579:591	circumscribed	579:591	circumscribed	579:591	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
28423949	5	48	with	spherical	671:679	arg1	efficiency					757:766	38 ± 1.43% entrapment efficiency	735:766	38 ± 1.43% entrapment efficiency	735:766	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	5	48	with	spherical	671:679	arg1	loading					723:729	23.28 ± 1.5% drug loading	705:729	23.28 ± 1.5% drug loading	705:729	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	5	48	with	spherical	671:679	arg1	size					699:702	198 ± 4.4 nm size	686:702	198 ± 4.4 nm size	686:702	NPs containing drug: polymer ratio (1.25:0.79) were spherical with 198 ± 4.4 nm size, 23.28 ± 1.5% drug loading and 38 ± 1.43% entrapment efficiency.
28423949	3	49	theme	intranasal	369:378	arg1	EFV-NPs					399:405	EFV-NPs	399:405	EFV-NPs	399:405	Hence, the present study is attempted to enhance the CNS uptake of efavirenz (EFV) by designing intranasal EFV nanoparticles (EFV-NPs).
28423949	3	49	theme	intranasal	369:378	arg1	nanoparticles					384:396	intranasal EFV nanoparticles	369:396	intranasal EFV nanoparticles (EFV-NPs)	369:406	Hence, the present study is attempted to enhance the CNS uptake of efavirenz (EFV) by designing intranasal EFV nanoparticles (EFV-NPs).
28423949	8	50	theme	Enhanced	963:970	arg1	bioavailability					976:990	Enhanced CNS bioavailability	963:990	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs	963:1143	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	8	51	theme	percentage	1054:1063	arg1	bioavailability					976:990	Enhanced CNS bioavailability	963:990	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs	963:1143	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	9	52	theme	scintigraphy	1199:1210	arg1	images					1212:1217	gamma scintigraphy images	1193:1217	gamma scintigraphy images	1193:1217	These results are corroborated by gamma scintigraphy images, which revealed high CNS uptake.
28423949	8	53	theme	administration	1123:1136	arg1	bioavailability					976:990	Enhanced CNS bioavailability	963:990	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs	963:1143	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	0	54	theme	CNS	63:65	arg1	targeting					67:75	the CNS targeting	59:75	the CNS targeting	59:75	Intranasal chitosan-g-HPβCD nanoparticles of efavirenz for the CNS targeting.
28423949	8	55	theme	post-intranasal	1107:1121	arg1	administration					1123:1136	drug-targeting index (141.3) post-intranasal administration	1078:1136	drug-targeting index (141.3) post-intranasal administration of NPs	1078:1143	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	6	56	from	%	816:816	arg1	8 h					821:823	8 h	821:823	8 h	821:823	NPs showed sustained drug release (99.03 ± 0.30% in 8 h) and followed Fickian diffusion mechanism.
28423949	4	57	theme	response	511:518	arg1	methodology					528:538	quadratic response surface methodology	501:538	quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design	501:616	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
28423949	4	57	theme	response	511:518	arg1	RSM					541:543	RSM	541:543	RSM	541:543	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
28423949	3	58	theme	present	284:290	arg1	study					292:296	the present study	280:296	the present study	280:296	Hence, the present study is attempted to enhance the CNS uptake of efavirenz (EFV) by designing intranasal EFV nanoparticles (EFV-NPs).
28423949	1	59	theme	complete	119:126	arg1	eradication					128:138	complete eradication	119:138	complete eradication of HIV from the CNS	119:158	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	7	60	theme	4.76	876:879	arg1	times					881:885	times	881:885	times	881:885	It gave 4.76 times greater permeability than plain drug solution through porcine nasal mucosa.
28423949	4	61	theme	quadratic	501:509	arg1	methodology					528:538	quadratic response surface methodology	501:538	quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design	501:616	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
28423949	4	61	theme	quadratic	501:509	arg1	RSM					541:543	RSM	541:543	RSM	541:543	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
28423949	2	62	theme	poor	248:251	arg1	penetration					253:263	the poor penetration	244:263	the poor penetration of ARV	244:270	The ineffectiveness is largely due to the poor penetration of ARV.
28423949	6	63	theme	drug	790:793	arg1	release					795:801	sustained drug release	780:801	sustained drug release (99.03 ± 0.30% in 8 h)	780:824	NPs showed sustained drug release (99.03 ± 0.30% in 8 h) and followed Fickian diffusion mechanism.
28423949	10	64	theme	L929	1324:1327	arg1	line					1334:1337	L929 cell line	1324:1337	L929 cell line	1324:1337	NPs appeared histocompatible with porcine nasal mucosa and non-toxic to L929 cell line.
28423949	10	65	theme	nasal	1294:1298	arg1	mucosa					1300:1305	porcine nasal mucosa	1286:1305	porcine nasal mucosa	1286:1305	NPs appeared histocompatible with porcine nasal mucosa and non-toxic to L929 cell line.
28423949	11	66	theme	potential	1369:1377	arg1	carrier					1379:1385	a potential carrier	1367:1385	a potential carrier in developing intranasal mucoadhesive EFV-NPs for the CNS targeting	1367:1453	Thus, CS-g-HPβCD served as a potential carrier in developing intranasal mucoadhesive EFV-NPs for the CNS targeting.
28423949	11	66	theme	potential	1369:1377	arg1	CS-g-HPβCD					1346:1355	CS-g-HPβCD	1346:1355	CS-g-HPβCD	1346:1355	Thus, CS-g-HPβCD served as a potential carrier in developing intranasal mucoadhesive EFV-NPs for the CNS targeting.
28423949	11	67	theme	CNS	1441:1443	arg1	targeting					1445:1453	the CNS targeting	1437:1453	the CNS targeting	1437:1453	Thus, CS-g-HPβCD served as a potential carrier in developing intranasal mucoadhesive EFV-NPs for the CNS targeting.
28423949	8	68	theme	index	1093:1097	arg1	administration					1123:1136	drug-targeting index (141.3) post-intranasal administration	1078:1136	drug-targeting index (141.3) post-intranasal administration of NPs	1078:1143	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	9	69	theme	high	1235:1238	arg1	uptake					1244:1249	high CNS uptake	1235:1249	high CNS uptake	1235:1249	These results are corroborated by gamma scintigraphy images, which revealed high CNS uptake.
28423949	1	70	from	issue	175:179	arg1	treatment					195:203	neuro-AIDS treatment	184:203	neuro-AIDS treatment	184:203	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	1	71	theme	HIV	143:145	arg1	eradication					128:138	complete eradication	119:138	complete eradication of HIV from the CNS	119:158	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	4	72	theme	composite	601:609	arg1	design					611:616	two-factor, five-level circumscribed central composite design	556:616	two-factor, five-level circumscribed central composite design	556:616	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
28423949	8	73	theme	drug-targeting	1078:1091	arg1	administration					1123:1136	drug-targeting index (141.3) post-intranasal administration	1078:1136	drug-targeting index (141.3) post-intranasal administration of NPs	1078:1143	Enhanced CNS bioavailability (12.40-fold that of i.v solution) of EFV, high drug-targeting percentage (99.24%) and drug-targeting index (141.3) post-intranasal administration of NPs was observed.
28423949	4	74	theme	gelation	465:472	arg1	method					474:479	ionic gelation method	459:479	ionic gelation method	459:479	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
28423949	7	75	theme	nasal	949:953	arg1	mucosa					955:960	porcine nasal mucosa	941:960	porcine nasal mucosa	941:960	It gave 4.76 times greater permeability than plain drug solution through porcine nasal mucosa.
28423949	1	76	from	Incompetence	78:89	arg1	eradication					128:138	complete eradication	119:138	complete eradication of HIV from the CNS	119:158	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	1	76	from	Incompetence	78:89	arg1	CNS					156:158	the CNS	152:158	the CNS	152:158	Incompetence of antiretrovirals (ARV) in complete eradication of HIV from the CNS is the biggest issue in neuro-AIDS treatment.
28423949	4	77	theme	central	593:599	arg1	design					611:616	two-factor, five-level circumscribed central composite design	556:616	two-factor, five-level circumscribed central composite design	556:616	EFV-NPs were fabricated using chitosan-g-HPβCD by ionic gelation method and optimized using quadratic response surface methodology (RSM) employing two-factor, five-level circumscribed central composite design.
25300473	0	0	theme	guanine-based	99:111	arg1	drugs					113:117	guanine-based drugs	99:117	guanine-based drugs	99:117	β-Cyclodextrin-grafted on multiwalled carbon nanotubes as versatile nanoplatform for entrapment of guanine-based drugs.
25300473	6	1	theme	antiviral	930:938	arg1	data					940:943	The preliminary antiviral data	914:943	The preliminary antiviral data	914:943	The preliminary antiviral data indicated that the Acyclovir loaded into the β-CD-MWCNT platform interferes with HSV-1 replication and the antireplicative effect was higher than the free drug.
25300473	3	2	theme	drug	507:510	arg1	abilities					520:528	The drug binding abilities	503:528	The drug binding abilities of nanocarrier towards the guanine (G) and Acyclovir (Acy)	503:587	The drug binding abilities of nanocarrier towards the guanine (G) and Acyclovir (Acy) were proved by UV-vis and DSC experiments.
25300473	6	3	theme	HSV-1	1026:1030	arg1	replication					1032:1042	HSV-1 replication	1026:1042	HSV-1 replication	1026:1042	The preliminary antiviral data indicated that the Acyclovir loaded into the β-CD-MWCNT platform interferes with HSV-1 replication and the antireplicative effect was higher than the free drug.
25300473	2	4	theme	chemical	481:488	arg1	composition					490:500	chemical composition	481:500	chemical composition	481:500	The functionalized carbon nanomaterials have been characterized by XPS spectroscopy, electron microscopy (FEG-SEM and TEM), AFM, TGA, and FT-IR to achieve insights on structure, morphology and chemical composition.
25300473	6	5	theme	preliminary	918:928	arg1	data					940:943	The preliminary antiviral data	914:943	The preliminary antiviral data	914:943	The preliminary antiviral data indicated that the Acyclovir loaded into the β-CD-MWCNT platform interferes with HSV-1 replication and the antireplicative effect was higher than the free drug.
25300473	3	6	theme	binding	512:518	arg1	abilities					520:528	The drug binding abilities	503:528	The drug binding abilities of nanocarrier towards the guanine (G) and Acyclovir (Acy)	503:587	The drug binding abilities of nanocarrier towards the guanine (G) and Acyclovir (Acy) were proved by UV-vis and DSC experiments.
25300473	2	7	from	insights	443:450	arg1	morphology					466:475	morphology	466:475	morphology	466:475	The functionalized carbon nanomaterials have been characterized by XPS spectroscopy, electron microscopy (FEG-SEM and TEM), AFM, TGA, and FT-IR to achieve insights on structure, morphology and chemical composition.
25300473	2	7	from	insights	443:450	arg1	composition					490:500	chemical composition	481:500	chemical composition	481:500	The functionalized carbon nanomaterials have been characterized by XPS spectroscopy, electron microscopy (FEG-SEM and TEM), AFM, TGA, and FT-IR to achieve insights on structure, morphology and chemical composition.
25300473	2	7	from	insights	443:450	arg1	structure					455:463	structure	455:463	structure	455:463	The functionalized carbon nanomaterials have been characterized by XPS spectroscopy, electron microscopy (FEG-SEM and TEM), AFM, TGA, and FT-IR to achieve insights on structure, morphology and chemical composition.
25300473	0	8	theme	drugs	113:117	arg1	entrapment					85:94	entrapment	85:94	entrapment of guanine-based drugs	85:117	β-Cyclodextrin-grafted on multiwalled carbon nanotubes as versatile nanoplatform for entrapment of guanine-based drugs.
25300473	4	9	theme	G/β-CD-MWCNT	718:729	arg1	complexes					750:758	G/β-CD-MWCNT and Acy/β-CD-MWCNT complexes	718:758	G/β-CD-MWCNT and Acy/β-CD-MWCNT complexes	718:758	Host-guest equilibrium association constants and drug loading have been evaluated for G/β-CD-MWCNT and Acy/β-CD-MWCNT complexes.
25300473	4	10	theme	Acy/β-CD-MWCNT	735:748	arg1	complexes					750:758	G/β-CD-MWCNT and Acy/β-CD-MWCNT complexes	718:758	G/β-CD-MWCNT and Acy/β-CD-MWCNT complexes	718:758	Host-guest equilibrium association constants and drug loading have been evaluated for G/β-CD-MWCNT and Acy/β-CD-MWCNT complexes.
25300473	2	11	theme	carbon	307:312	arg1	nanomaterials					314:326	The functionalized carbon nanomaterials	288:326	The functionalized carbon nanomaterials	288:326	The functionalized carbon nanomaterials have been characterized by XPS spectroscopy, electron microscopy (FEG-SEM and TEM), AFM, TGA, and FT-IR to achieve insights on structure, morphology and chemical composition.
25300473	6	12	theme	free	1095:1098	arg1	drug					1100:1103	the free drug	1091:1103	the free drug	1091:1103	The preliminary antiviral data indicated that the Acyclovir loaded into the β-CD-MWCNT platform interferes with HSV-1 replication and the antireplicative effect was higher than the free drug.
25300473	4	13	theme	equilibrium	643:653	arg1	constants					667:675	Host-guest equilibrium association constants	632:675	Host-guest equilibrium association constants	632:675	Host-guest equilibrium association constants and drug loading have been evaluated for G/β-CD-MWCNT and Acy/β-CD-MWCNT complexes.
25300473	2	14	theme	functionalized	292:305	arg1	nanomaterials					314:326	The functionalized carbon nanomaterials	288:326	The functionalized carbon nanomaterials	288:326	The functionalized carbon nanomaterials have been characterized by XPS spectroscopy, electron microscopy (FEG-SEM and TEM), AFM, TGA, and FT-IR to achieve insights on structure, morphology and chemical composition.
25300473	4	15	theme	Host-guest	632:641	arg1	constants					667:675	Host-guest equilibrium association constants	632:675	Host-guest equilibrium association constants	632:675	Host-guest equilibrium association constants and drug loading have been evaluated for G/β-CD-MWCNT and Acy/β-CD-MWCNT complexes.
25300473	5	16	theme	Acy	812:814	arg1	delivery					800:807	a sustained delivery	788:807	a sustained delivery of Acy	788:814	The release studies showed a sustained delivery of Acy without initial burst effect confirming a strong interaction of drug with the nanoplatform sites.
25300473	2	17	theme	XPS	355:357	arg1	spectroscopy					359:370	XPS spectroscopy	355:370	XPS spectroscopy	355:370	The functionalized carbon nanomaterials have been characterized by XPS spectroscopy, electron microscopy (FEG-SEM and TEM), AFM, TGA, and FT-IR to achieve insights on structure, morphology and chemical composition.
25300473	5	18	theme	drug	880:883	arg1	interaction					865:875	a strong interaction	856:875	a strong interaction of drug with the nanoplatform sites	856:911	The release studies showed a sustained delivery of Acy without initial burst effect confirming a strong interaction of drug with the nanoplatform sites.
25300473	6	19	theme	antireplicative	1052:1066	arg1	higher					1079:1084	higher	1079:1084	higher	1079:1084	The preliminary antiviral data indicated that the Acyclovir loaded into the β-CD-MWCNT platform interferes with HSV-1 replication and the antireplicative effect was higher than the free drug.
25300473	6	19	theme	antireplicative	1052:1066	arg1	effect					1068:1073	the antireplicative effect	1048:1073	the antireplicative effect	1048:1073	The preliminary antiviral data indicated that the Acyclovir loaded into the β-CD-MWCNT platform interferes with HSV-1 replication and the antireplicative effect was higher than the free drug.
25300473	6	20	theme	β-CD-MWCNT	990:999	arg1	platform					1001:1008	the β-CD-MWCNT platform	986:1008	the β-CD-MWCNT platform	986:1008	The preliminary antiviral data indicated that the Acyclovir loaded into the β-CD-MWCNT platform interferes with HSV-1 replication and the antireplicative effect was higher than the free drug.
25300473	4	21	theme	association	655:665	arg1	constants					667:675	Host-guest equilibrium association constants	632:675	Host-guest equilibrium association constants	632:675	Host-guest equilibrium association constants and drug loading have been evaluated for G/β-CD-MWCNT and Acy/β-CD-MWCNT complexes.
25300473	0	22	theme	carbon	38:43	arg1	nanotubes					45:53	multiwalled carbon nanotubes	26:53	multiwalled carbon nanotubes as versatile nanoplatform for entrapment of guanine-based drugs	26:117	β-Cyclodextrin-grafted on multiwalled carbon nanotubes as versatile nanoplatform for entrapment of guanine-based drugs.
25300473	2	23	dep	microscopy	382:391	arg1	FEG-SEM					394:400	FEG-SEM	394:400	FEG-SEM	394:400	The functionalized carbon nanomaterials have been characterized by XPS spectroscopy, electron microscopy (FEG-SEM and TEM), AFM, TGA, and FT-IR to achieve insights on structure, morphology and chemical composition.
25300473	2	23	dep	microscopy	382:391	arg1	TEM					406:408	TEM	406:408	TEM	406:408	The functionalized carbon nanomaterials have been characterized by XPS spectroscopy, electron microscopy (FEG-SEM and TEM), AFM, TGA, and FT-IR to achieve insights on structure, morphology and chemical composition.
25300473	0	24	theme	multiwalled	26:36	arg1	nanotubes					45:53	multiwalled carbon nanotubes	26:53	multiwalled carbon nanotubes as versatile nanoplatform for entrapment of guanine-based drugs	26:117	β-Cyclodextrin-grafted on multiwalled carbon nanotubes as versatile nanoplatform for entrapment of guanine-based drugs.
25300473	3	25	theme	nanocarrier	533:543	arg1	abilities					520:528	The drug binding abilities	503:528	The drug binding abilities of nanocarrier towards the guanine (G) and Acyclovir (Acy)	503:587	The drug binding abilities of nanocarrier towards the guanine (G) and Acyclovir (Acy) were proved by UV-vis and DSC experiments.
25300473	4	26	theme	drug	681:684	arg1	loading					686:692	drug loading	681:692	drug loading	681:692	Host-guest equilibrium association constants and drug loading have been evaluated for G/β-CD-MWCNT and Acy/β-CD-MWCNT complexes.
25300473	2	27	theme	electron	373:380	arg1	microscopy					382:391	electron microscopy	373:391	electron microscopy (FEG-SEM and TEM)	373:409	The functionalized carbon nanomaterials have been characterized by XPS spectroscopy, electron microscopy (FEG-SEM and TEM), AFM, TGA, and FT-IR to achieve insights on structure, morphology and chemical composition.
25300473	5	28	theme	sustained	790:798	arg1	delivery					800:807	a sustained delivery	788:807	a sustained delivery of Acy	788:814	The release studies showed a sustained delivery of Acy without initial burst effect confirming a strong interaction of drug with the nanoplatform sites.
25300473	5	29	theme	initial	824:830	arg1	effect					838:843	initial burst effect	824:843	initial burst effect	824:843	The release studies showed a sustained delivery of Acy without initial burst effect confirming a strong interaction of drug with the nanoplatform sites.
25300473	5	30	theme	strong	858:863	arg1	interaction					865:875	a strong interaction	856:875	a strong interaction of drug with the nanoplatform sites	856:911	The release studies showed a sustained delivery of Acy without initial burst effect confirming a strong interaction of drug with the nanoplatform sites.
25300473	3	31	theme	DSC	615:617	arg1	experiments					619:629	UV-vis and DSC experiments	604:629	UV-vis and DSC experiments	604:629	The drug binding abilities of nanocarrier towards the guanine (G) and Acyclovir (Acy) were proved by UV-vis and DSC experiments.
25300473	0	32	theme	versatile	58:66	arg1	nanoplatform					68:79	versatile nanoplatform	58:79	versatile nanoplatform for entrapment of guanine-based drugs	58:117	β-Cyclodextrin-grafted on multiwalled carbon nanotubes as versatile nanoplatform for entrapment of guanine-based drugs.
25300473	1	33	theme	β-cyclodextrin/multiwalled	134:159	arg1	β-CD-MWCNT					186:195	β-CD-MWCNT	186:195	β-CD-MWCNT	186:195	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	1	33	theme	β-cyclodextrin/multiwalled	134:159	arg1	hybrid					178:183	β-cyclodextrin/multiwalled carbon nanotubes hybrid	134:183	β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs	134:267	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	5	34	with	interaction	865:875	arg1	sites					907:911	the nanoplatform sites	890:911	the nanoplatform sites	890:911	The release studies showed a sustained delivery of Acy without initial burst effect confirming a strong interaction of drug with the nanoplatform sites.
25300473	1	35	theme	carbon	161:166	arg1	β-CD-MWCNT					186:195	β-CD-MWCNT	186:195	β-CD-MWCNT	186:195	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	1	35	theme	carbon	161:166	arg1	hybrid					178:183	β-cyclodextrin/multiwalled carbon nanotubes hybrid	134:183	β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs	134:267	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	1	36	theme	guanine	249:255	arg1	drugs					263:267	guanine based drugs	249:267	guanine based drugs	249:267	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	3	37	theme	UV-vis	604:609	arg1	experiments					619:629	UV-vis and DSC experiments	604:629	UV-vis and DSC experiments	604:629	The drug binding abilities of nanocarrier towards the guanine (G) and Acyclovir (Acy) were proved by UV-vis and DSC experiments.
25300473	1	38	theme	nanotubes	168:176	arg1	β-CD-MWCNT					186:195	β-CD-MWCNT	186:195	β-CD-MWCNT	186:195	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	1	38	theme	nanotubes	168:176	arg1	hybrid					178:183	β-cyclodextrin/multiwalled carbon nanotubes hybrid	134:183	β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs	134:267	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	1	39	theme	based	257:261	arg1	drugs					263:267	guanine based drugs	249:267	guanine based drugs	249:267	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	1	40	dep	entrapment	222:231	arg1	the					218:220	the	218:220	the	218:220	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	1	41	theme	hybrid	178:183	arg1	design					124:129	The design	120:129	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs	120:267	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	1	42	theme	drugs	263:267	arg1	delivery					237:244	delivery	237:244	delivery	237:244	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	1	42	theme	drugs	263:267	arg1	entrapment					222:231	entrapment	222:231	entrapment	222:231	The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here.
25300473	5	43	theme	release	765:771	arg1	studies					773:779	The release studies	761:779	The release studies	761:779	The release studies showed a sustained delivery of Acy without initial burst effect confirming a strong interaction of drug with the nanoplatform sites.
25300473	5	44	theme	burst	832:836	arg1	effect					838:843	initial burst effect	824:843	initial burst effect	824:843	The release studies showed a sustained delivery of Acy without initial burst effect confirming a strong interaction of drug with the nanoplatform sites.
25300473	5	45	theme	nanoplatform	894:905	arg1	sites					907:911	the nanoplatform sites	890:911	the nanoplatform sites	890:911	The release studies showed a sustained delivery of Acy without initial burst effect confirming a strong interaction of drug with the nanoplatform sites.
27916570	3	0	theme	renewable	557:565	arg1	polymers					567:574	these two renewable polymers	547:574	these two renewable polymers	547:574	The furan-modified natural rubber used in this strategy was also exploited to bind it to maleimide-modified nanocellulose, thus generating a covalently crosslinked composite of these two renewable polymers.
27916570	2	1	theme	maleimide	350:358	arg1	moieties					360:367	furan and maleimide moieties	340:367	furan and maleimide moieties	340:367	We demonstrate here that natural rubber can be reversibly crosslinked via the Diels-Alder coupling of furan and maleimide moieties.
27916570	3	2	theme	crosslinked	522:532	arg1	polymers					567:574	these two renewable polymers	547:574	these two renewable polymers	547:574	The furan-modified natural rubber used in this strategy was also exploited to bind it to maleimide-modified nanocellulose, thus generating a covalently crosslinked composite of these two renewable polymers.
27916570	3	2	theme	crosslinked	522:532	arg1	composite					534:542	a covalently crosslinked composite	509:542	a covalently crosslinked composite of these two renewable polymers	509:574	The furan-modified natural rubber used in this strategy was also exploited to bind it to maleimide-modified nanocellulose, thus generating a covalently crosslinked composite of these two renewable polymers.
27916570	3	3	theme	polymers	567:574	arg1	polymers					567:574	these two renewable polymers	547:574	these two renewable polymers	547:574	The furan-modified natural rubber used in this strategy was also exploited to bind it to maleimide-modified nanocellulose, thus generating a covalently crosslinked composite of these two renewable polymers.
27916570	3	3	theme	polymers	567:574	arg1	composite					534:542	a covalently crosslinked composite	509:542	a covalently crosslinked composite of these two renewable polymers	509:574	The furan-modified natural rubber used in this strategy was also exploited to bind it to maleimide-modified nanocellulose, thus generating a covalently crosslinked composite of these two renewable polymers.
27916570	1	4	theme	conventional	119:130	arg1	irreversible					179:190	irreversible	179:190	irreversible	179:190	The conventional vulcanization process applied to elastomers is irreversible and hinders therefore their useful recycling.
27916570	1	4	theme	conventional	119:130	arg1	process					146:152	The conventional vulcanization process	115:152	The conventional vulcanization process applied to elastomers	115:174	The conventional vulcanization process applied to elastomers is irreversible and hinders therefore their useful recycling.
27916570	3	5	theme	maleimide-modified	459:476	arg1	nanocellulose					478:490	maleimide-modified nanocellulose	459:490	maleimide-modified nanocellulose	459:490	The furan-modified natural rubber used in this strategy was also exploited to bind it to maleimide-modified nanocellulose, thus generating a covalently crosslinked composite of these two renewable polymers.
27916570	0	6	theme	natural	15:21	arg1	rubber					23:28	Furan-modified natural rubber	0:28	Furan-modified natural rubber: A substrate for its reversible crosslinking and for clicking it onto nanocellulose.	0:113	Furan-modified natural rubber: A substrate for its reversible crosslinking and for clicking it onto nanocellulose.
27916570	3	7	link	crosslinked	522:532	arg1	polymers					567:574	these two renewable polymers	547:574	these two renewable polymers	547:574	The furan-modified natural rubber used in this strategy was also exploited to bind it to maleimide-modified nanocellulose, thus generating a covalently crosslinked composite of these two renewable polymers.
27916570	3	7	link	crosslinked	522:532	arg1	composite					534:542	a covalently crosslinked composite	509:542	a covalently crosslinked composite of these two renewable polymers	509:574	The furan-modified natural rubber used in this strategy was also exploited to bind it to maleimide-modified nanocellulose, thus generating a covalently crosslinked composite of these two renewable polymers.
27916570	1	8	theme	useful	220:225	arg1	recycling					227:235	their useful recycling	214:235	their useful recycling	214:235	The conventional vulcanization process applied to elastomers is irreversible and hinders therefore their useful recycling.
27916570	0	9	dep	rubber	23:28	arg1	substrate					33:41	A substrate	31:41	Furan-modified natural rubber: A substrate for its reversible crosslinking and for clicking it onto nanocellulose.	0:113	Furan-modified natural rubber: A substrate for its reversible crosslinking and for clicking it onto nanocellulose.
27916570	2	10	attach	crosslinked	296:306	arg3	coupling					328:335	the Diels-Alder coupling	312:335	the Diels-Alder coupling of furan and maleimide moieties	312:367	We demonstrate here that natural rubber can be reversibly crosslinked via the Diels-Alder coupling of furan and maleimide moieties.
27916570	1	11	theme	vulcanization	132:144	arg1	irreversible					179:190	irreversible	179:190	irreversible	179:190	The conventional vulcanization process applied to elastomers is irreversible and hinders therefore their useful recycling.
27916570	1	11	theme	vulcanization	132:144	arg1	process					146:152	The conventional vulcanization process	115:152	The conventional vulcanization process applied to elastomers	115:174	The conventional vulcanization process applied to elastomers is irreversible and hinders therefore their useful recycling.
27916570	0	12	theme	Furan-modified	0:13	arg1	rubber					23:28	Furan-modified natural rubber	0:28	Furan-modified natural rubber: A substrate for its reversible crosslinking and for clicking it onto nanocellulose.	0:113	Furan-modified natural rubber: A substrate for its reversible crosslinking and for clicking it onto nanocellulose.
27916570	2	13	theme	natural	263:269	arg1	rubber					271:276	natural rubber	263:276	natural rubber	263:276	We demonstrate here that natural rubber can be reversibly crosslinked via the Diels-Alder coupling of furan and maleimide moieties.
27916570	3	14	theme	furan-modified	374:387	arg1	rubber					397:402	The furan-modified natural rubber	370:402	The furan-modified natural rubber used in this strategy	370:424	The furan-modified natural rubber used in this strategy was also exploited to bind it to maleimide-modified nanocellulose, thus generating a covalently crosslinked composite of these two renewable polymers.
27916570	0	15	theme	reversible	51:60	arg1	crosslinking					62:73	its reversible crosslinking	47:73	its reversible crosslinking	47:73	Furan-modified natural rubber: A substrate for its reversible crosslinking and for clicking it onto nanocellulose.
27916570	2	16	theme	moieties	360:367	arg1	coupling					328:335	the Diels-Alder coupling	312:335	the Diels-Alder coupling of furan and maleimide moieties	312:367	We demonstrate here that natural rubber can be reversibly crosslinked via the Diels-Alder coupling of furan and maleimide moieties.
27916570	2	17	theme	furan	340:344	arg1	moieties					360:367	furan and maleimide moieties	340:367	furan and maleimide moieties	340:367	We demonstrate here that natural rubber can be reversibly crosslinked via the Diels-Alder coupling of furan and maleimide moieties.
27916570	2	18	theme	Diels-Alder	316:326	arg1	coupling					328:335	the Diels-Alder coupling	312:335	the Diels-Alder coupling of furan and maleimide moieties	312:367	We demonstrate here that natural rubber can be reversibly crosslinked via the Diels-Alder coupling of furan and maleimide moieties.
27916570	3	19	theme	natural	389:395	arg1	rubber					397:402	The furan-modified natural rubber	370:402	The furan-modified natural rubber used in this strategy	370:424	The furan-modified natural rubber used in this strategy was also exploited to bind it to maleimide-modified nanocellulose, thus generating a covalently crosslinked composite of these two renewable polymers.
24699819	5	0	dep	other	861:865	arg1	constitutive					880:891	constitutive	880:891	constitutive	880:891	Instead, it inhibits the production of these, along with nearly all other (induced and constitutive) proteins that transit through the ER.
24699819	5	0	dep	other	861:865	arg1	induced					868:874	induced	868:874	induced	868:874	Instead, it inhibits the production of these, along with nearly all other (induced and constitutive) proteins that transit through the ER.
24699819	2	1	theme	effects	354:360	arg1	Many					342:345	Many	342:345	Many	342:345	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	2	1	theme	effects	354:360	arg1	effects					354:360	its effects	350:360	its effects	350:360	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	12	2	from	cells	1702:1706	arg1	proteins					1680:1687	glycosylated and secreted proteins	1654:1687	glycosylated and secreted proteins from treated cells	1654:1706	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	12	2	from	cells	1702:1706	arg1	loss					1646:1649	a near-complete loss	1630:1649	a near-complete loss of glycosylated and secreted proteins from treated cells	1630:1706	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	4	3	theme	polysome	651:658	arg1	profiling					660:668	polysome profiling	651:668	polysome profiling	651:668	Using polysome profiling we now demonstrate conclusively that mycolactone does not prevent translation of TNF, IL-6 and Cox-2 mRNAs in macrophages.
24699819	13	4	theme	production	1816:1825	arg1	lack					1774:1777	the profound lack	1761:1777	the profound lack of glycosylated and secreted protein production	1761:1825	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	13	4	theme	production	1816:1825	arg1	apparent					1830:1837	apparent	1830:1837	apparent	1830:1837	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	14	5	theme	observed	1955:1962	arg1	activities					1977:1986	mycolactone's observed pathological activities	1941:1986	mycolactone's observed pathological activities both in vitro and in vivo	1941:2012	These studies provide a new mechanism underlying mycolactone's observed pathological activities both in vitro and in vivo.
24699819	8	6	theme	proteasome	1213:1222	arg1	action					1195:1200	the action	1191:1200	the action of the 26S proteasome	1191:1222	First, cellular TNF and Cox-2 can be once more detected if the action of the 26S proteasome is inhibited concurrently.
24699819	13	7	theme	glycosylated	1782:1793	arg1	production					1816:1825	glycosylated and secreted protein production	1782:1825	glycosylated and secreted protein production	1782:1825	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	10	8	theme	proteins	1437:1444	arg1	translocation					1406:1418	the translocation	1402:1418	the translocation of TNF and other proteins into the ER	1402:1456	Third, in vitro translation assays show mycolactone prevents the translocation of TNF and other proteins into the ER.
24699819	3	9	theme	mediators	611:619	arg1	production					567:576	the production	563:576	the production of LPS-dependent proinflammatory mediators	563:619	We have previously shown mycolactone completely blocks the production of LPS-dependent proinflammatory mediators post-transcriptionally.
24699819	15	10	theme	cytokines	2207:2215	arg1	loss					2149:2152	the loss	2145:2152	the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer	2145:2256	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	13	11	theme	secreted	1799:1806	arg1	production					1816:1825	glycosylated and secreted protein production	1782:1825	glycosylated and secreted protein production	1782:1825	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	0	12	theme	protein	109:115	arg1	translocation					117:129	protein translocation	109:129	protein translocation into the ER	109:141	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
24699819	9	13	theme	restored	1259:1266	arg1	protein					1268:1274	restored protein	1259:1274	restored protein	1259:1274	Second, restored protein is found in the cytosol, indicating an inability to translocate.
24699819	3	14	theme	LPS-dependent	581:593	arg1	mediators					611:619	LPS-dependent proinflammatory mediators	581:619	LPS-dependent proinflammatory mediators	581:619	We have previously shown mycolactone completely blocks the production of LPS-dependent proinflammatory mediators post-transcriptionally.
24699819	1	15	theme	tissue	202:207	arg1	necrosis					209:216	tissue necrosis	202:216	tissue necrosis	202:216	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	12	16	theme	near-complete	1632:1644	arg1	loss					1646:1649	a near-complete loss	1630:1649	a near-complete loss of glycosylated and secreted proteins from treated cells	1630:1706	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	7	17	theme	mycolactone	1110:1120	arg1	function					1122:1129	mycolactone function	1110:1129	mycolactone function	1110:1129	Several lines of evidence support this transformative explanation of mycolactone function.
24699819	15	18	theme	adhesion	2177:2184	arg1	molecules					2186:2194	adhesion molecules	2177:2194	adhesion molecules	2177:2194	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	6	19	theme	protein	961:967	arg1	translocation					969:981	protein translocation	961:981	protein translocation	961:981	This is due to a blockade of protein translocation and subsequent degradation of aberrantly located protein.
24699819	13	20	from	range	1844:1848	arg1	apparent					1830:1837	apparent	1830:1837	apparent	1830:1837	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	13	20	from	range	1844:1848	arg1	lack					1774:1777	the profound lack	1761:1777	the profound lack of glycosylated and secreted protein production	1761:1825	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	7	21	theme	Several	1041:1047	arg1	lines					1049:1053	Several lines	1041:1053	Several lines of evidence	1041:1065	Several lines of evidence support this transformative explanation of mycolactone function.
24699819	12	22	theme	metabolic	1602:1610	arg1	labelling					1612:1620	metabolic labelling	1602:1620	metabolic labelling	1602:1620	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	11	23	theme	tail-anchored	1496:1508	arg1	proteins					1510:1517	tail-anchored proteins	1496:1517	tail-anchored proteins	1496:1517	This is specific as the insertion of tail-anchored proteins into the ER is unaffected showing that the ER remains structurally intact.
24699819	0	24	theme	factor	65:70	arg1	mechanism					15:23	The pathogenic mechanism	0:23	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone,	0:84	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
24699819	15	25	theme	Buruli	2245:2250	arg1	ulcer					2252:2256	Buruli ulcer	2245:2256	Buruli ulcer	2245:2256	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	6	26	theme	protein	1032:1038	arg1	blockade					949:956	a blockade	947:956	a blockade of protein translocation	947:981	This is due to a blockade of protein translocation and subsequent degradation of aberrantly located protein.
24699819	6	26	theme	protein	1032:1038	arg1	degradation					998:1008	subsequent degradation	987:1008	subsequent degradation of aberrantly located protein	987:1038	This is due to a blockade of protein translocation and subsequent degradation of aberrantly located protein.
24699819	15	27	theme	cytokines	2125:2133	arg1	deficit					2107:2113	the deficit	2103:2113	the deficit of innate cytokines	2103:2133	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	2	28	theme	specific	402:409	arg1	proteins					411:418	specific proteins	402:418	specific proteins implicated in important cellular processes, such as immune responses and cell adhesion	402:505	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	0	29	theme	Mycobacterium	32:44	arg1	mycolactone					73:83	mycolactone	73:83	mycolactone	73:83	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
24699819	0	29	theme	Mycobacterium	32:44	arg1	factor					65:70	the Mycobacterium ulcerans virulence factor	28:70	the Mycobacterium ulcerans virulence factor	28:70	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
24699819	4	30	dep	TNF	751:753	arg1	mRNAs					771:775	mRNAs	771:775	mRNAs	771:775	Using polysome profiling we now demonstrate conclusively that mycolactone does not prevent translation of TNF, IL-6 and Cox-2 mRNAs in macrophages.
24699819	1	31	theme	sufficient	278:287	arg1	factor					299:304	the necessary and sufficient virulence factor	260:304	the necessary and sufficient virulence factor for Buruli ulcer disease pathology	260:339	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	1	31	theme	sufficient	278:287	arg1	mycolactone					247:257	mycolactone	247:257	mycolactone	247:257	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	12	32	theme	glycosylated	1654:1665	arg1	proteins					1680:1687	glycosylated and secreted proteins	1654:1687	glycosylated and secreted proteins from treated cells	1654:1706	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	13	33	theme	disease-relevant	1863:1878	arg1	types					1885:1889	different disease-relevant cell types	1853:1889	different disease-relevant cell types	1853:1889	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	2	34	theme	important	434:442	arg1	responses					479:487	immune responses	472:487	immune responses	472:487	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	2	34	theme	important	434:442	arg1	processes					453:461	important cellular processes	434:461	important cellular processes	434:461	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	2	34	theme	important	434:442	arg1	adhesion					498:505	cell adhesion	493:505	cell adhesion	493:505	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	12	35	theme	secreted	1671:1678	arg1	proteins					1680:1687	glycosylated and secreted proteins	1654:1687	glycosylated and secreted proteins from treated cells	1654:1706	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	10	36	dep	show	1376:1379	arg1	prevents					1393:1400	prevents	1393:1400	show mycolactone prevents the translocation of TNF and other proteins into the ER	1376:1456	Third, in vitro translation assays show mycolactone prevents the translocation of TNF and other proteins into the ER.
24699819	15	37	theme	translocation	2059:2071	arg1	inhibition					2037:2046	Mycolactone-dependent inhibition	2015:2046	Mycolactone-dependent inhibition of protein translocation into the ER	2015:2083	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	13	38	theme	types	1885:1889	arg1	range					1844:1848	a range	1842:1848	a range of different disease-relevant cell types	1842:1889	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	14	39	theme	pathological	1964:1975	arg1	activities					1977:1986	mycolactone's observed pathological activities	1941:1986	mycolactone's observed pathological activities both in vitro and in vivo	1941:2012	These studies provide a new mechanism underlying mycolactone's observed pathological activities both in vitro and in vivo.
24699819	1	40	theme	Buruli	310:315	arg1	disease					323:329	Buruli ulcer disease	310:329	Buruli ulcer disease pathology	310:339	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	8	41	theme	cellular	1139:1146	arg1	TNF					1148:1150	TNF	1148:1150	TNF	1148:1150	First, cellular TNF and Cox-2 can be once more detected if the action of the 26S proteasome is inhibited concurrently.
24699819	15	42	theme	receptors	2166:2174	arg1	loss					2149:2152	the loss	2145:2152	the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer	2145:2256	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	2	43	theme	immune	472:477	arg1	responses					479:487	immune responses	472:487	immune responses	472:487	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	10	44	theme	translation	1357:1367	arg1	assays					1369:1374	in vitro translation assays	1348:1374	in vitro translation assays	1348:1374	Third, in vitro translation assays show mycolactone prevents the translocation of TNF and other proteins into the ER.
24699819	0	45	theme	ulcerans	46:53	arg1	mycolactone					73:83	mycolactone	73:83	mycolactone	73:83	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
24699819	0	45	theme	ulcerans	46:53	arg1	factor					65:70	the Mycobacterium ulcerans virulence factor	28:70	the Mycobacterium ulcerans virulence factor	28:70	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
24699819	1	46	theme	disease	323:329	arg1	pathology					331:339	Buruli ulcer disease pathology	310:339	Buruli ulcer disease pathology	310:339	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	15	47	theme	Mycolactone-dependent	2015:2035	arg1	inhibition					2037:2046	Mycolactone-dependent inhibition	2015:2046	Mycolactone-dependent inhibition of protein translocation into the ER	2015:2083	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	10	48	theme	in	1348:1349	arg1	assays					1369:1374	in vitro translation assays	1348:1374	in vitro translation assays	1348:1374	Third, in vitro translation assays show mycolactone prevents the translocation of TNF and other proteins into the ER.
24699819	9	49	located	found	1279:1283	arg1	cytosol					1292:1298	the cytosol	1288:1298	the cytosol	1288:1298	Second, restored protein is found in the cytosol, indicating an inability to translocate.
24699819	9	49	located	found	1279:1283	arg2	protein					1268:1274	restored protein	1259:1274	restored protein	1259:1274	Second, restored protein is found in the cytosol, indicating an inability to translocate.
24699819	4	50	dep	demonstrate	677:687	arg1	Using					645:649	Using	645:649	Using polysome profiling	645:668	Using polysome profiling we now demonstrate conclusively that mycolactone does not prevent translation of TNF, IL-6 and Cox-2 mRNAs in macrophages.
24699819	13	51	gly	glycosylated	1782:1793	arg1	production					1816:1825	glycosylated and secreted protein production	1782:1825	glycosylated and secreted protein production	1782:1825	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	13	52	theme	protein	1808:1814	arg1	production					1816:1825	glycosylated and secreted protein production	1782:1825	glycosylated and secreted protein production	1782:1825	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	10	53	theme	TNF	1423:1425	arg1	translocation					1406:1418	the translocation	1402:1418	the translocation of TNF and other proteins into the ER	1402:1456	Third, in vitro translation assays show mycolactone prevents the translocation of TNF and other proteins into the ER.
24699819	1	54	theme	necessary	264:272	arg1	factor					299:304	the necessary and sufficient virulence factor	260:304	the necessary and sufficient virulence factor for Buruli ulcer disease pathology	260:339	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	1	54	theme	necessary	264:272	arg1	mycolactone					247:257	mycolactone	247:257	mycolactone	247:257	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	4	55	from	translation	736:746	arg1	macrophages					780:790	macrophages	780:790	macrophages	780:790	Using polysome profiling we now demonstrate conclusively that mycolactone does not prevent translation of TNF, IL-6 and Cox-2 mRNAs in macrophages.
24699819	1	56	theme	Mycobacterium	159:171	arg1	ulcerans					173:180	Mycobacterium ulcerans	159:180	Mycobacterium ulcerans	159:180	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	12	57	dep	reveals	1622:1628	arg1	whereas					1709:1715	whereas	1709:1715	whereas	1709:1715	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	3	58	theme	proinflammatory	595:609	arg1	mediators					611:619	LPS-dependent proinflammatory mediators	581:619	LPS-dependent proinflammatory mediators	581:619	We have previously shown mycolactone completely blocks the production of LPS-dependent proinflammatory mediators post-transcriptionally.
24699819	15	59	theme	protein	2051:2057	arg1	translocation					2059:2071	protein translocation	2051:2071	protein translocation into the ER	2051:2083	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	15	60	theme	T-cell	2200:2205	arg1	cytokines					2207:2215	T-cell cytokines	2200:2215	T-cell cytokines	2200:2215	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	8	61	theme	26S	1209:1211	arg1	proteasome					1213:1222	the 26S proteasome	1205:1222	the 26S proteasome	1205:1222	First, cellular TNF and Cox-2 can be once more detected if the action of the 26S proteasome is inhibited concurrently.
24699819	0	62	theme	translocation	117:129	arg1	blockade					97:104	blockade	97:104	blockade of protein translocation into the ER	97:141	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
24699819	10	63	theme	other	1431:1435	arg1	proteins					1437:1444	other proteins	1431:1444	other proteins	1431:1444	Third, in vitro translation assays show mycolactone prevents the translocation of TNF and other proteins into the ER.
24699819	10	64	dep	in	1348:1349	arg1	vitro					1351:1355	vitro	1351:1355	vitro	1351:1355	Third, in vitro translation assays show mycolactone prevents the translocation of TNF and other proteins into the ER.
24699819	15	65	theme	molecules	2186:2194	arg1	loss					2149:2152	the loss	2145:2152	the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer	2145:2256	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	6	66	theme	subsequent	987:996	arg1	degradation					998:1008	subsequent degradation	987:1008	subsequent degradation of aberrantly located protein	987:1038	This is due to a blockade of protein translocation and subsequent degradation of aberrantly located protein.
24699819	13	67	from	apparent	1830:1837	arg1	range					1844:1848	a range	1842:1848	a range of different disease-relevant cell types	1842:1889	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	14	68	theme	new	1916:1918	arg1	mechanism					1920:1928	a new mechanism	1914:1928	a new mechanism underlying mycolactone's observed pathological activities both in vitro and in vivo	1914:2012	These studies provide a new mechanism underlying mycolactone's observed pathological activities both in vitro and in vivo.
24699819	6	69	theme	translocation	969:981	arg1	blockade					949:956	a blockade	947:956	a blockade of protein translocation	947:981	This is due to a blockade of protein translocation and subsequent degradation of aberrantly located protein.
24699819	6	69	theme	translocation	969:981	arg1	degradation					998:1008	subsequent degradation	987:1008	subsequent degradation of aberrantly located protein	987:1038	This is due to a blockade of protein translocation and subsequent degradation of aberrantly located protein.
24699819	13	70	theme	profound	1765:1772	arg1	lack					1774:1777	the profound lack	1761:1777	the profound lack of glycosylated and secreted protein production	1761:1825	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	13	70	theme	profound	1765:1772	arg1	apparent					1830:1837	apparent	1830:1837	apparent	1830:1837	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	7	71	theme	transformative	1080:1093	arg1	explanation					1095:1105	this transformative explanation	1075:1105	this transformative explanation of mycolactone function	1075:1129	Several lines of evidence support this transformative explanation of mycolactone function.
24699819	7	72	theme	evidence	1058:1065	arg1	lines					1049:1053	Several lines	1041:1053	Several lines of evidence	1041:1065	Several lines of evidence support this transformative explanation of mycolactone function.
24699819	7	73	theme	function	1122:1129	arg1	explanation					1095:1105	this transformative explanation	1075:1105	this transformative explanation of mycolactone function	1075:1129	Several lines of evidence support this transformative explanation of mycolactone function.
24699819	4	74	theme	Cox-2	765:769	arg1	translation					736:746	translation	736:746	translation of TNF, IL-6 and Cox-2 mRNAs in macrophages	736:790	Using polysome profiling we now demonstrate conclusively that mycolactone does not prevent translation of TNF, IL-6 and Cox-2 mRNAs in macrophages.
24699819	11	75	theme	proteins	1510:1517	arg1	insertion					1483:1491	the insertion	1479:1491	the insertion of tail-anchored proteins into the ER	1479:1529	This is specific as the insertion of tail-anchored proteins into the ER is unaffected showing that the ER remains structurally intact.
24699819	15	76	theme	ulcer	2252:2256	arg1	aetiology					2232:2240	the aetiology	2228:2240	the aetiology of Buruli ulcer	2228:2256	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	4	77	theme	TNF	751:753	arg1	translation					736:746	translation	736:746	translation of TNF, IL-6 and Cox-2 mRNAs in macrophages	736:790	Using polysome profiling we now demonstrate conclusively that mycolactone does not prevent translation of TNF, IL-6 and Cox-2 mRNAs in macrophages.
24699819	1	78	with	Infection	144:152	arg1	ulcerans					173:180	Mycobacterium ulcerans	159:180	Mycobacterium ulcerans	159:180	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	2	79	theme	cell	493:496	arg1	adhesion					498:505	cell adhesion	493:505	cell adhesion	493:505	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	4	80	theme	IL-6	756:759	arg1	translation					736:746	translation	736:746	translation of TNF, IL-6 and Cox-2 mRNAs in macrophages	736:790	Using polysome profiling we now demonstrate conclusively that mycolactone does not prevent translation of TNF, IL-6 and Cox-2 mRNAs in macrophages.
24699819	15	81	theme	innate	2118:2123	arg1	cytokines					2125:2133	innate cytokines	2118:2133	innate cytokines	2118:2133	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	12	82	theme	cytosolic	1717:1725	arg1	proteins					1727:1734	cytosolic proteins	1717:1734	cytosolic proteins	1717:1734	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	6	83	theme	located	1024:1030	arg1	protein					1032:1038	aberrantly located protein	1013:1038	aberrantly located protein	1013:1038	This is due to a blockade of protein translocation and subsequent degradation of aberrantly located protein.
24699819	12	84	theme	proteins	1680:1687	arg1	loss					1646:1649	a near-complete loss	1630:1649	a near-complete loss of glycosylated and secreted proteins from treated cells	1630:1706	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	0	85	theme	pathogenic	4:13	arg1	mechanism					15:23	The pathogenic mechanism	0:23	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone,	0:84	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
24699819	12	86	theme	treated	1694:1700	arg1	cells					1702:1706	treated cells	1694:1706	treated cells	1694:1706	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	13	87	theme	different	1853:1861	arg1	types					1885:1889	different disease-relevant cell types	1853:1889	different disease-relevant cell types	1853:1889	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	5	88	theme	other	861:865	arg1	proteins					894:901	nearly all other (induced and constitutive) proteins	850:901	nearly all other (induced and constitutive) proteins that transit through the ER	850:929	Instead, it inhibits the production of these, along with nearly all other (induced and constitutive) proteins that transit through the ER.
24699819	1	89	theme	virulence	289:297	arg1	factor					299:304	the necessary and sufficient virulence factor	260:304	the necessary and sufficient virulence factor for Buruli ulcer disease pathology	260:339	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	1	89	theme	virulence	289:297	arg1	mycolactone					247:257	mycolactone	247:257	mycolactone	247:257	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	12	90	from	loss	1646:1649	arg1	cells					1702:1706	treated cells	1694:1706	treated cells	1694:1706	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	2	91	theme	cellular	444:451	arg1	responses					479:487	immune responses	472:487	immune responses	472:487	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	2	91	theme	cellular	444:451	arg1	processes					453:461	important cellular processes	434:461	important cellular processes	434:461	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	2	91	theme	cellular	444:451	arg1	adhesion					498:505	cell adhesion	493:505	cell adhesion	493:505	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	13	92	theme	cell	1880:1883	arg1	types					1885:1889	different disease-relevant cell types	1853:1889	different disease-relevant cell types	1853:1889	Notably, the profound lack of glycosylated and secreted protein production is apparent in a range of different disease-relevant cell types.
24699819	5	93	theme	these	832:836	arg1	production					818:827	the production	814:827	the production of these	814:836	Instead, it inhibits the production of these, along with nearly all other (induced and constitutive) proteins that transit through the ER.
24699819	15	94	theme	membrane	2157:2164	arg1	receptors					2166:2174	membrane receptors	2157:2174	membrane receptors	2157:2174	Mycolactone-dependent inhibition of protein translocation into the ER not only explains the deficit of innate cytokines, but also the loss of membrane receptors, adhesion molecules and T-cell cytokines that drive the aetiology of Buruli ulcer.
24699819	2	95	theme	proteins	411:418	arg1	down-regulation					383:397	down-regulation	383:397	down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion	383:505	Many of its effects are known to involve down-regulation of specific proteins implicated in important cellular processes, such as immune responses and cell adhesion.
24699819	0	96	theme	virulence	55:63	arg1	mycolactone					73:83	mycolactone	73:83	mycolactone	73:83	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
24699819	0	96	theme	virulence	55:63	arg1	factor					65:70	the Mycobacterium ulcerans virulence factor	28:70	the Mycobacterium ulcerans virulence factor	28:70	The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
24699819	1	97	theme	ulcer	317:321	arg1	disease					323:329	Buruli ulcer disease	310:329	Buruli ulcer disease pathology	310:339	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
24699819	12	98	gly	glycosylated	1654:1665	arg1	proteins					1680:1687	glycosylated and secreted proteins	1654:1687	glycosylated and secreted proteins from treated cells	1654:1706	Fourth, metabolic labelling reveals a near-complete loss of glycosylated and secreted proteins from treated cells, whereas cytosolic proteins are unaffected.
24699819	1	99	theme	due	240:242	arg1	necrosis					209:216	tissue necrosis	202:216	tissue necrosis	202:216	Infection with Mycobacterium ulcerans is characterised by tissue necrosis and immunosuppression due to mycolactone, the necessary and sufficient virulence factor for Buruli ulcer disease pathology.
26969875	2	0	from	effect	199:204	arg1	properties					271:280	rheological properties	259:280	rheological properties	259:280	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	2	0	from	effect	199:204	arg1	quality					298:304	final bread quality	286:304	final bread quality	286:304	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	2	1	theme	final	286:290	arg1	quality					298:304	final bread quality	286:304	final bread quality	286:304	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	7	2	theme	flour	1319:1323	arg1	dough					1325:1329	refined and 100% wheat flour dough	1296:1329	refined and 100% wheat flour dough samples	1296:1337	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	6	3	theme	loaf	1042:1045	arg1	volume					1047:1052	higher loaf volume	1035:1052	higher loaf volume	1035:1052	Bread made from composite frozen dough had higher loaf volume compared to ref A or ref B throughout the storage period.
26969875	4	4	theme	A	575:575	arg1	samples					554:560	Dough samples	548:560	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B)	548:677	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	5	5	from	spectroscopy	978:989	arg1	data					903:906	data	903:906	data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy	903:989	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	1	6	theme	bread-making	173:184	arg1	quality					186:192	bread-making quality	173:192	bread-making quality	173:192	BACKGROUND The rheological characteristics of frozen dough are of great importance in bread-making quality.
26969875	7	7	theme	frozen	1269:1274	arg1	storage					1276:1282	frozen storage	1269:1282	frozen storage	1269:1282	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	4	8	theme	B	581:581	arg1	samples					554:560	Dough samples	548:560	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B)	548:677	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	6	9	theme	storage	1096:1102	arg1	period					1104:1109	the storage period	1092:1109	the storage period	1092:1109	Bread made from composite frozen dough had higher loaf volume compared to ref A or ref B throughout the storage period.
26969875	2	10	theme	frozen	309:314	arg1	dough					316:320	frozen dough	309:320	frozen dough	309:320	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	6	11	theme	frozen	1018:1023	arg1	dough					1025:1029	composite frozen dough	1008:1029	composite frozen dough	1008:1029	Bread made from composite frozen dough had higher loaf volume compared to ref A or ref B throughout the storage period.
26969875	4	12	theme	Dough	548:552	arg1	samples					554:560	Dough samples	548:560	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B)	548:677	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	5	13	theme	water	792:796	arg1	water					814:818	freezable water	804:818	freezable water	804:818	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	13	theme	water	792:796	arg1	amounts					775:781	higher amounts	768:781	higher amounts of bound water, less freezable water	768:818	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	13	theme	water	792:796	arg1	water					792:796	bound water	786:796	bound water	786:796	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	14	theme	differential	913:924	arg1	calorimetry					935:945	differential scanning calorimetry	913:945	differential scanning calorimetry	913:945	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	3	15	theme	kg-1	489:492	arg1	level					507:511	150 g kg-1 substitution level	483:511	150 g kg-1 substitution level (composite A and B, respectively)	483:545	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	2	16	theme	wheat	232:236	arg1	aleurone					238:245	commercial wheat aleurone	221:245	commercial wheat aleurone	221:245	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	7	17	theme	rheological	1228:1238	arg1	properties					1240:1249	its rheological properties	1224:1249	its rheological properties during 9 weeks of frozen storage	1224:1282	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	4	18	theme	dough	612:616	arg1	samples					618:624	two reference dough samples	598:624	two reference dough samples	598:624	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	18	theme	dough	612:616	arg1	flour					635:639	refined flour	627:639	refined flour (ref A)	627:647	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	18	theme	dough	612:616	arg1	flour					665:669	whole wheat flour	653:669	whole wheat flour (ref B)	653:677	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	3	19	theme	composite	514:522	arg1	A					524:524	composite A	514:524	composite A	514:524	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	5	20	theme	higher	768:773	arg1	water					814:818	freezable water	804:818	freezable water	804:818	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	20	theme	higher	768:773	arg1	amounts					775:781	higher amounts	768:781	higher amounts of bound water, less freezable water	768:818	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	20	theme	higher	768:773	arg1	water					792:796	bound water	786:796	bound water	786:796	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	21	contain	contained	758:766	arg2	water					814:818	freezable water	804:818	freezable water	804:818	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	21	contain	contained	758:766	arg1	samples					750:756	RESULT Frozen stored composite dough samples	713:756	RESULT Frozen stored composite dough samples	713:756	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	21	contain	contained	758:766	arg2	water					792:796	bound water	786:796	bound water	786:796	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	21	contain	contained	758:766	arg2	amounts					775:781	higher amounts	768:781	higher amounts of bound water, less freezable water	768:818	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	1	22	theme	dough	140:144	arg1	characteristics					114:128	The rheological characteristics	98:128	The rheological characteristics of frozen dough	98:144	BACKGROUND The rheological characteristics of frozen dough are of great importance in bread-making quality.
26969875	6	23	theme	ref	1066:1068	arg1	A					1070:1070	ref A	1066:1070	ref A	1066:1070	Bread made from composite frozen dough had higher loaf volume compared to ref A or ref B throughout the storage period.
26969875	5	24	theme	composite	734:742	arg1	samples					750:756	RESULT Frozen stored composite dough samples	713:756	RESULT Frozen stored composite dough samples	713:756	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	0	25	theme	wheat	74:78	arg1	fiber					80:84	added wheat fiber	68:84	added wheat fiber	68:84	Rheological properties and bread quality of frozen yeast-dough with added wheat fiber.
26969875	3	26	dep	level	507:511	arg1	A					524:524	composite A	514:524	composite A	514:524	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	3	26	dep	level	507:511	arg1	B					530:530	B	530:530	B	530:530	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	7	27	dep	CONCLUSION	1112:1121	arg1	compared					1284:1291	compared	1284:1291	compared to refined and 100% wheat flour dough samples	1284:1337	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	5	28	theme	nuclear	951:957	arg1	resonance					968:976	nuclear magnetic resonance	951:976	nuclear magnetic resonance spectroscopy	951:989	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	29	theme	water	814:818	arg1	water					814:818	freezable water	804:818	freezable water	804:818	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	29	theme	water	814:818	arg1	amounts					775:781	higher amounts	768:781	higher amounts of bound water, less freezable water	768:818	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	29	theme	water	814:818	arg1	water					792:796	bound water	786:796	bound water	786:796	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	4	30	theme	whole	653:657	arg1	samples					618:624	two reference dough samples	598:624	two reference dough samples	598:624	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	30	theme	whole	653:657	arg1	flour					665:669	whole wheat flour	653:669	whole wheat flour (ref B)	653:677	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	30	theme	whole	653:657	arg1	B					676:676	ref B	672:676	ref B	672:676	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	3	31	theme	200	393:395	arg1	g					397:397	g	397:397	g	397:397	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	5	32	theme	resonance	968:976	arg1	spectroscopy					978:989	nuclear magnetic resonance spectroscopy	951:989	nuclear magnetic resonance spectroscopy	951:989	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	0	33	theme	Rheological	0:10	arg1	properties					12:21	Rheological properties	0:21	Rheological properties	0:21	Rheological properties and bread quality of frozen yeast-dough with added wheat fiber.
26969875	7	34	dep	dough	1325:1329	arg1	%					1311:1311	%	1311:1311	%	1311:1311	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	4	35	dep	samples	618:624	arg1	addition					586:593	addition	586:593	addition	586:593	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	7	36	theme	produced	1181:1188	arg1	dough					1190:1194	refined wheat flour produced dough	1161:1194	refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage	1161:1282	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	7	37	theme	minimum	1201:1207	arg1	alterations					1209:1219	minimum alterations	1201:1219	minimum alterations in its rheological properties during 9 weeks of frozen storage	1201:1282	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	4	38	theme	ref	642:644	arg1	flour					635:639	refined flour	627:639	refined flour (ref A)	627:647	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	38	theme	ref	642:644	arg1	A					646:646	ref A	642:646	ref A	642:646	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	1	39	theme	rheological	102:112	arg1	characteristics					114:128	The rheological characteristics	98:128	The rheological characteristics of frozen dough	98:144	BACKGROUND The rheological characteristics of frozen dough are of great importance in bread-making quality.
26969875	7	40	theme	wheat	1169:1173	arg1	dough					1190:1194	refined wheat flour produced dough	1161:1194	refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage	1161:1282	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	3	41	theme	240	378:380	arg1	g					382:382	g	382:382	g	382:382	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	0	42	theme	frozen	44:49	arg1	yeast-dough					51:61	frozen yeast-dough	44:61	frozen yeast-dough	44:61	Rheological properties and bread quality of frozen yeast-dough with added wheat fiber.
26969875	7	43	theme	wheat	1144:1148	arg1	fiber					1150:1154	wheat fiber	1144:1154	wheat fiber	1144:1154	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	3	44	dep	arabinoxylan	404:415	arg1	AX					418:419	AX	418:419	AX	418:419	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	4	45	theme	ref	672:674	arg1	flour					665:669	whole wheat flour	653:669	whole wheat flour (ref B)	653:677	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	45	theme	ref	672:674	arg1	B					676:676	ref B	672:676	ref B	672:676	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	5	46	theme	gluten	857:862	arg1	network					864:870	gluten network	857:870	gluten network	857:870	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	0	47	with	quality	33:39	arg1	fiber					80:84	added wheat fiber	68:84	added wheat fiber	68:84	Rheological properties and bread quality of frozen yeast-dough with added wheat fiber.
26969875	7	48	theme	dough	1325:1329	arg1	samples					1331:1337	refined and 100% wheat flour dough samples	1296:1337	refined and 100% wheat flour dough samples	1296:1337	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	3	49	contain	containing	367:376	arg2	kg-1					384:387	240 g kg-1	378:387	240 g kg-1	378:387	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	3	49	contain	containing	367:376	arg1	A					351:351	A	351:351	A	351:351	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	3	49	contain	containing	367:376	arg2	kg-1					399:402	200 g kg-1	393:402	200 g kg-1	393:402	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	3	49	contain	containing	367:376	arg1	aleurone					341:348	Wheat aleurone (A) and bran (B)	335:365	aleurone	341:348	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	2	50	theme	bread	292:296	arg1	quality					298:304	final bread quality	286:304	final bread quality	286:304	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	2	51	theme	dough	316:320	arg1	properties					271:280	rheological properties	259:280	rheological properties	259:280	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	2	51	theme	dough	316:320	arg1	quality					298:304	final bread quality	286:304	final bread quality	286:304	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	7	52	theme	wheat	1313:1317	arg1	dough					1325:1329	refined and 100% wheat flour dough	1296:1329	refined and 100% wheat flour dough samples	1296:1337	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	5	53	theme	dough	744:748	arg1	samples					750:756	RESULT Frozen stored composite dough samples	713:756	RESULT Frozen stored composite dough samples	713:756	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	6	54	contain	had	1031:1033	arg1	Bread					992:996	Bread	992:996	Bread made from composite frozen dough	992:1029	Bread made from composite frozen dough had higher loaf volume compared to ref A or ref B throughout the storage period.
26969875	6	54	contain	had	1031:1033	arg2	volume					1047:1052	higher loaf volume	1035:1052	higher loaf volume	1035:1052	Bread made from composite frozen dough had higher loaf volume compared to ref A or ref B throughout the storage period.
26969875	3	55	theme	wheat	468:472	arg1	flour					474:478	refined wheat flour	460:478	refined wheat flour	460:478	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	6	56	theme	higher	1035:1040	arg1	volume					1047:1052	higher loaf volume	1035:1052	higher loaf volume	1035:1052	Bread made from composite frozen dough had higher loaf volume compared to ref A or ref B throughout the storage period.
26969875	4	57	theme	composite	565:573	arg1	A					575:575	composite A	565:575	composite A	565:575	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	3	58	theme	substitution	494:505	arg1	level					507:511	150 g kg-1 substitution level	483:511	150 g kg-1 substitution level (composite A and B, respectively)	483:545	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	5	59	from	modifications	840:852	arg1	network					864:870	gluten network	857:870	gluten network	857:870	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	60	theme	RESULT	713:718	arg1	samples					750:756	RESULT Frozen stored composite dough samples	713:756	RESULT Frozen stored composite dough samples	713:756	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	7	61	theme	storage	1276:1282	arg1	weeks					1260:1264	9 weeks	1258:1264	9 weeks of frozen storage	1258:1282	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	3	62	theme	g	487:487	arg1	level					507:511	150 g kg-1 substitution level	483:511	150 g kg-1 substitution level (composite A and B, respectively)	483:545	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	6	63	theme	composite	1008:1016	arg1	dough					1025:1029	composite frozen dough	1008:1029	composite frozen dough	1008:1029	Bread made from composite frozen dough had higher loaf volume compared to ref A or ref B throughout the storage period.
26969875	5	64	theme	scanning	926:933	arg1	calorimetry					935:945	differential scanning calorimetry	913:945	differential scanning calorimetry	913:945	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	2	65	theme	aleurone	238:245	arg1	addition					209:216	addition	209:216	addition of commercial wheat aleurone and bran	209:254	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	2	66	theme	commercial	221:230	arg1	aleurone					238:245	commercial wheat aleurone	221:245	commercial wheat aleurone	221:245	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	6	67	theme	ref	1075:1077	arg1	B					1079:1079	ref B	1075:1079	ref B	1075:1079	Bread made from composite frozen dough had higher loaf volume compared to ref A or ref B throughout the storage period.
26969875	4	68	theme	reference	602:610	arg1	samples					618:624	two reference dough samples	598:624	two reference dough samples	598:624	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	68	theme	reference	602:610	arg1	flour					635:639	refined flour	627:639	refined flour (ref A)	627:647	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	68	theme	reference	602:610	arg1	flour					665:669	whole wheat flour	653:669	whole wheat flour (ref B)	653:677	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	7	69	with	dough	1190:1194	arg1	alterations					1209:1219	minimum alterations	1201:1219	minimum alterations in its rheological properties during 9 weeks of frozen storage	1201:1282	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	2	70	theme	rheological	259:269	arg1	properties					271:280	rheological properties	259:280	rheological properties	259:280	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	1	71	theme	frozen	133:138	arg1	dough					140:144	frozen dough	133:144	frozen dough	133:144	BACKGROUND The rheological characteristics of frozen dough are of great importance in bread-making quality.
26969875	0	72	theme	added	68:72	arg1	fiber					80:84	added wheat fiber	68:84	added wheat fiber	68:84	Rheological properties and bread quality of frozen yeast-dough with added wheat fiber.
26969875	2	73	theme	bran	251:254	arg1	addition					209:216	addition	209:216	addition of commercial wheat aleurone and bran	209:254	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	5	74	theme	stored	727:732	arg1	samples					750:756	RESULT Frozen stored composite dough samples	713:756	RESULT Frozen stored composite dough samples	713:756	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	3	75	theme	Wheat	335:339	arg1	A					351:351	A	351:351	A	351:351	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	3	75	theme	Wheat	335:339	arg1	aleurone					341:348	Wheat aleurone (A) and bran (B)	335:365	aleurone	341:348	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	1	76	theme	great	153:157	arg1	importance					159:168	great importance	153:168	great importance	153:168	BACKGROUND The rheological characteristics of frozen dough are of great importance in bread-making quality.
26969875	8	77	theme	Chemical	1358:1365	arg1	Industry					1367:1374	Chemical Industry	1358:1374	Chemical Industry	1358:1374	© 2016 Society of Chemical Industry.
26969875	1	78	dep	BACKGROUND	87:96	arg1	importance					159:168	great importance	153:168	great importance	153:168	BACKGROUND The rheological characteristics of frozen dough are of great importance in bread-making quality.
26969875	5	79	from	calorimetry	935:945	arg1	data					903:906	data	903:906	data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy	903:989	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	1	80	from	importance	159:168	arg1	quality					186:192	bread-making quality	173:192	bread-making quality	173:192	BACKGROUND The rheological characteristics of frozen dough are of great importance in bread-making quality.
26969875	5	81	theme	freezable	804:812	arg1	water					814:818	freezable water	804:818	freezable water	804:818	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	5	82	theme	magnetic	959:966	arg1	resonance					968:976	nuclear magnetic resonance	951:976	nuclear magnetic resonance spectroscopy	951:989	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	7	83	from	alterations	1209:1219	arg1	properties					1240:1249	its rheological properties	1224:1249	its rheological properties during 9 weeks of frozen storage	1224:1282	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	3	84	theme	g	397:397	arg1	kg-1					399:402	200 g kg-1	393:402	200 g kg-1	393:402	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	5	85	theme	bound	786:790	arg1	water					792:796	bound water	786:796	bound water	786:796	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	7	86	theme	flour	1175:1179	arg1	dough					1190:1194	refined wheat flour produced dough	1161:1194	refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage	1161:1282	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	4	87	theme	refined	627:633	arg1	samples					618:624	two reference dough samples	598:624	two reference dough samples	598:624	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	87	theme	refined	627:633	arg1	flour					635:639	refined flour	627:639	refined flour (ref A)	627:647	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	87	theme	refined	627:633	arg1	A					646:646	ref A	642:646	ref A	642:646	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	0	88	theme	bread	27:31	arg1	quality					33:39	bread quality	27:39	bread quality of frozen yeast-dough with added wheat fiber	27:84	Rheological properties and bread quality of frozen yeast-dough with added wheat fiber.
26969875	7	89	theme	refined	1161:1167	arg1	dough					1190:1194	refined wheat flour produced dough	1161:1194	refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage	1161:1282	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	3	90	dep	kg-1	384:387	arg1	arabinoxylan					404:415	arabinoxylan	404:415	arabinoxylan (AX)	404:420	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	7	91	theme	fiber	1150:1154	arg1	incorporation					1127:1139	The incorporation	1123:1139	The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage	1123:1282	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	3	92	theme	g	382:382	arg1	kg-1					384:387	240 g kg-1	378:387	240 g kg-1	378:387	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
26969875	0	93	theme	yeast-dough	51:61	arg1	properties					12:21	Rheological properties	0:21	Rheological properties	0:21	Rheological properties and bread quality of frozen yeast-dough with added wheat fiber.
26969875	0	93	theme	yeast-dough	51:61	arg1	quality					33:39	bread quality	27:39	bread quality of frozen yeast-dough with added wheat fiber	27:84	Rheological properties and bread quality of frozen yeast-dough with added wheat fiber.
26969875	7	94	dep	%	1311:1311	arg1	100					1308:1310	100	1308:1310	100	1308:1310	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	7	94	dep	%	1311:1311	arg1	refined					1296:1302	refined	1296:1302	refined	1296:1302	CONCLUSION The incorporation of wheat fiber into refined wheat flour produced dough with minimum alterations in its rheological properties during 9 weeks of frozen storage compared to refined and 100% wheat flour dough samples.
26969875	5	95	theme	frozen	879:884	arg1	storage					886:892	frozen storage	879:892	frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy	879:989	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	2	96	theme	addition	209:216	arg1	effect					199:204	The effect	195:204	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough	195:320	The effect of addition of commercial wheat aleurone and bran on rheological properties and final bread quality of frozen dough was studied.
26969875	0	97	with	properties	12:21	arg1	fiber					80:84	added wheat fiber	68:84	added wheat fiber	68:84	Rheological properties and bread quality of frozen yeast-dough with added wheat fiber.
26969875	5	98	theme	fewer	834:838	arg1	modifications					840:852	fewer modifications	834:852	fewer modifications in gluten network	834:870	RESULT Frozen stored composite dough samples contained higher amounts of bound water, less freezable water and exhibited fewer modifications in gluten network during frozen storage based on data from differential scanning calorimetry and nuclear magnetic resonance spectroscopy.
26969875	4	99	theme	wheat	659:663	arg1	samples					618:624	two reference dough samples	598:624	two reference dough samples	598:624	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	99	theme	wheat	659:663	arg1	flour					665:669	whole wheat flour	653:669	whole wheat flour (ref B)	653:677	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	4	99	theme	wheat	659:663	arg1	B					676:676	ref B	672:676	ref B	672:676	Dough samples of composite A and B in addition to two reference dough samples, refined flour (ref A) and whole wheat flour (ref B) were stored at -18°C for 9 weeks.
26969875	3	100	theme	refined	460:466	arg1	flour					474:478	refined wheat flour	460:478	refined wheat flour	460:478	Wheat aleurone (A) and bran (B) containing 240 g kg-1 and 200 g kg-1 arabinoxylan (AX), respectively, were incorporated into refined wheat flour at 150 g kg-1 substitution level (composite A and B, respectively).
24601217	3	0	theme	curing	686:691	arg1	calcium					725:731	calcium	725:731	calcium	725:731	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	0	theme	curing	686:691	arg1	zinc					719:722	zinc	719:722	zinc	719:722	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	0	theme	curing	686:691	arg1	ions					713:716	curing cross-linking metal ions	686:716	curing cross-linking metal ions (zinc, calcium and aluminum)	686:745	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	0	theme	curing	686:691	arg1	aluminum					737:744	aluminum	737:744	aluminum	737:744	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	7	1	link	Calcium-crosslinked	1260:1278	arg1	beads					1303:1307	Calcium-crosslinked alginate-terephthalate beads	1260:1307	Calcium-crosslinked alginate-terephthalate beads	1260:1307	Calcium-crosslinked alginate-terephthalate beads illustrated enteric release behavior regardless of their curing temperature.
24601217	8	2	theme	zero	1602:1605	arg1	profiles					1621:1628	interesting zero order release profiles	1590:1628	interesting zero order release profiles	1590:1628	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	8	3	theme	aluminum-crosslinked	1532:1551	arg1	beads					1576:1580	aluminum-crosslinked alginate-terephthalate beads	1532:1580	aluminum-crosslinked alginate-terephthalate beads	1532:1580	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	8	4	theme	interesting	1590:1600	arg1	profiles					1621:1628	interesting zero order release profiles	1590:1628	interesting zero order release profiles	1590:1628	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	0	5	theme	controlled	114:123	arg1	matrices					133:140	potential controlled release matrices	104:140	potential controlled release matrices	104:140	Preparation of novel ionotropically crosslinked beads based on alginate-terephthalic acid composites as potential controlled release matrices.
24601217	7	6	theme	curing	1366:1371	arg1	temperature					1373:1383	their curing temperature	1360:1383	their curing temperature	1360:1383	Calcium-crosslinked alginate-terephthalate beads illustrated enteric release behavior regardless of their curing temperature.
24601217	4	7	theme	blue	845:848	arg1	model					879:883	loaded drug model	867:883	loaded drug model	867:883	Methylene blue (MB) was used as loaded drug model.
24601217	4	7	theme	blue	845:848	arg1	Methylene					835:843	Methylene blue	835:848	Methylene blue (MB)	835:853	Methylene blue (MB) was used as loaded drug model.
24601217	4	7	theme	blue	845:848	arg1	MB					851:852	MB	851:852	MB	851:852	Methylene blue (MB) was used as loaded drug model.
24601217	3	8	dep	loading	805:811	arg1	i.e.					794:797	i.e.	794:797	i.e.	794:797	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	8	9	theme	zinc-crosslinked	1405:1420	arg1	beads					1445:1449	zinc-crosslinked alginate-terephthalate beads	1405:1449	zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C.	1405:1521	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	3	10	theme	beads	787:791	arg1	characteristics					754:768	the characteristics	750:768	the characteristics	750:768	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	0	11	theme	potential	104:112	arg1	matrices					133:140	potential controlled release matrices	104:140	potential controlled release matrices	104:140	Preparation of novel ionotropically crosslinked beads based on alginate-terephthalic acid composites as potential controlled release matrices.
24601217	8	12	theme	release	1613:1619	arg1	profiles					1621:1628	interesting zero order release profiles	1590:1628	interesting zero order release profiles	1590:1628	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	7	13	theme	alginate-terephthalate	1280:1301	arg1	beads					1303:1307	Calcium-crosslinked alginate-terephthalate beads	1260:1307	Calcium-crosslinked alginate-terephthalate beads	1260:1307	Calcium-crosslinked alginate-terephthalate beads illustrated enteric release behavior regardless of their curing temperature.
24601217	2	14	theme	bridging	533:540	arg1	acid					498:501	terephthalic acid	485:501	terephthalic acid which is proposed to act as a bridging crosslinking aid	485:557	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	2	14	theme	bridging	533:540	arg1	crosslinking					542:553	a bridging crosslinking	531:553	a bridging crosslinking aid	531:557	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	8	15	theme	order	1607:1611	arg1	profiles					1621:1628	interesting zero order release profiles	1590:1628	interesting zero order release profiles	1590:1628	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	8	16	link	aluminum-crosslinked	1532:1551	arg1	beads					1576:1580	aluminum-crosslinked alginate-terephthalate beads	1532:1580	aluminum-crosslinked alginate-terephthalate beads	1532:1580	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	6	17	theme	MB	1133:1134	arg1	loadings					1136:1143	higher MB loadings	1126:1143	higher MB loadings	1126:1143	Interestingly, all prepared alginate-terephthalate beads (i.e., cured with zinc, calcium and aluminum) illustrated higher MB loadings and accessed controlled release profiles compared to their corresponding terephthalate-free alginate counterparts.
24601217	3	18	from	effect	628:633	arg1	characteristics					754:768	the characteristics	750:768	the characteristics	750:768	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	1	19	theme	poor	207:210	arg1	loading					217:223	poor drug loading	207:223	poor drug loading	207:223	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	0	20	theme	release	125:131	arg1	matrices					133:140	potential controlled release matrices	104:140	potential controlled release matrices	104:140	Preparation of novel ionotropically crosslinked beads based on alginate-terephthalic acid composites as potential controlled release matrices.
24601217	3	21	theme	cross-linking	693:705	arg1	calcium					725:731	calcium	725:731	calcium	725:731	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	21	theme	cross-linking	693:705	arg1	zinc					719:722	zinc	719:722	zinc	719:722	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	21	theme	cross-linking	693:705	arg1	ions					713:716	curing cross-linking metal ions	686:716	curing cross-linking metal ions (zinc, calcium and aluminum)	686:745	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	21	theme	cross-linking	693:705	arg1	aluminum					737:744	aluminum	737:744	aluminum	737:744	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	8	22	theme	release	1473:1479	arg1	profiles					1481:1488	enteric-like release profiles	1460:1488	enteric-like release profiles	1460:1488	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	6	23	theme	higher	1126:1131	arg1	loadings					1136:1143	higher MB loadings	1126:1143	higher MB loadings	1126:1143	Interestingly, all prepared alginate-terephthalate beads (i.e., cured with zinc, calcium and aluminum) illustrated higher MB loadings and accessed controlled release profiles compared to their corresponding terephthalate-free alginate counterparts.
24601217	1	24	theme	drug	212:215	arg1	loading					217:223	poor drug loading	207:223	poor drug loading	207:223	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	3	25	theme	metal	707:711	arg1	calcium					725:731	calcium	725:731	calcium	725:731	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	25	theme	metal	707:711	arg1	zinc					719:722	zinc	719:722	zinc	719:722	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	25	theme	metal	707:711	arg1	ions					713:716	curing cross-linking metal ions	686:716	curing cross-linking metal ions (zinc, calcium and aluminum)	686:745	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	25	theme	metal	707:711	arg1	aluminum					737:744	aluminum	737:744	aluminum	737:744	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	6	26	theme	alginate	1237:1244	arg1	counterparts					1246:1257	their corresponding terephthalate-free alginate counterparts	1198:1257	their corresponding terephthalate-free alginate counterparts	1198:1257	Interestingly, all prepared alginate-terephthalate beads (i.e., cured with zinc, calcium and aluminum) illustrated higher MB loadings and accessed controlled release profiles compared to their corresponding terephthalate-free alginate counterparts.
24601217	2	27	theme	present	335:341	arg1	work					343:346	the present work	331:346	the present work	331:346	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	6	28	theme	prepared	1030:1037	arg1	beads					1062:1066	all prepared alginate-terephthalate beads	1026:1066	all prepared alginate-terephthalate beads	1026:1066	Interestingly, all prepared alginate-terephthalate beads (i.e., cured with zinc, calcium and aluminum) illustrated higher MB loadings and accessed controlled release profiles compared to their corresponding terephthalate-free alginate counterparts.
24601217	1	29	theme	drug	297:300	arg1	profiles					310:317	drug release profiles	297:317	drug release profiles	297:317	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	6	30	theme	terephthalate-free	1218:1235	arg1	counterparts					1246:1257	their corresponding terephthalate-free alginate counterparts	1198:1257	their corresponding terephthalate-free alginate counterparts	1198:1257	Interestingly, all prepared alginate-terephthalate beads (i.e., cured with zinc, calcium and aluminum) illustrated higher MB loadings and accessed controlled release profiles compared to their corresponding terephthalate-free alginate counterparts.
24601217	3	31	theme	terephthalic	589:600	arg1	acid					602:605	terephthalic acid	589:605	terephthalic acid	589:605	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	7	32	theme	enteric	1321:1327	arg1	behavior					1337:1344	enteric release behavior	1321:1344	enteric release behavior	1321:1344	Calcium-crosslinked alginate-terephthalate beads illustrated enteric release behavior regardless of their curing temperature.
24601217	6	33	dep	illustrated	1114:1124	arg1	i.e.					1069:1072	i.e.	1069:1072	i.e.	1069:1072	Interestingly, all prepared alginate-terephthalate beads (i.e., cured with zinc, calcium and aluminum) illustrated higher MB loadings and accessed controlled release profiles compared to their corresponding terephthalate-free alginate counterparts.
24601217	1	34	theme	release	302:308	arg1	profiles					310:317	drug release profiles	297:317	drug release profiles	297:317	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	0	35	link	crosslinked	36:46	arg1	beads					48:52	novel ionotropically crosslinked beads	15:52	novel ionotropically crosslinked beads	15:52	Preparation of novel ionotropically crosslinked beads based on alginate-terephthalic acid composites as potential controlled release matrices.
24601217	8	36	theme	alginate-terephthalate	1422:1443	arg1	beads					1445:1449	zinc-crosslinked alginate-terephthalate beads	1405:1449	zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C.	1405:1521	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	6	37	theme	corresponding	1204:1216	arg1	counterparts					1246:1257	their corresponding terephthalate-free alginate counterparts	1198:1257	their corresponding terephthalate-free alginate counterparts	1198:1257	Interestingly, all prepared alginate-terephthalate beads (i.e., cured with zinc, calcium and aluminum) illustrated higher MB loadings and accessed controlled release profiles compared to their corresponding terephthalate-free alginate counterparts.
24601217	1	38	theme	poor	226:229	arg1	stability					231:239	poor stability	226:239	poor stability in biorelevant media	226:260	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	0	39	theme	novel	15:19	arg1	beads					48:52	novel ionotropically crosslinked beads	15:52	novel ionotropically crosslinked beads	15:52	Preparation of novel ionotropically crosslinked beads based on alginate-terephthalic acid composites as potential controlled release matrices.
24601217	8	40	theme	other	1393:1397	arg1	hand					1399:1402	the other hand	1389:1402	the other hand	1389:1402	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	4	41	theme	loaded	867:872	arg1	model					879:883	loaded drug model	867:883	loaded drug model	867:883	Methylene blue (MB) was used as loaded drug model.
24601217	4	41	theme	loaded	867:872	arg1	Methylene					835:843	Methylene blue	835:848	Methylene blue (MB)	835:853	Methylene blue (MB) was used as loaded drug model.
24601217	5	42	theme	resulting	890:898	arg1	beads					900:904	The resulting beads	886:904	The resulting beads	886:904	The resulting beads were chemically and physically probed using infrared spectroscopy and differential scanning calorimetry.
24601217	5	43	theme	differential	976:987	arg1	calorimetry					998:1008	differential scanning calorimetry	976:1008	differential scanning calorimetry	976:1008	The resulting beads were chemically and physically probed using infrared spectroscopy and differential scanning calorimetry.
24601217	2	44	theme	alginate	441:448	arg1	complexes					450:458	these metal ion-crosslinked alginate complexes	413:458	these metal ion-crosslinked alginate complexes	413:458	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	0	45	theme	beads	48:52	arg1	Preparation					0:10	Preparation	0:10	Preparation of novel ionotropically crosslinked beads	0:52	Preparation of novel ionotropically crosslinked beads based on alginate-terephthalic acid composites as potential controlled release matrices.
24601217	7	46	theme	release	1329:1335	arg1	behavior					1337:1344	enteric release behavior	1321:1344	enteric release behavior	1321:1344	Calcium-crosslinked alginate-terephthalate beads illustrated enteric release behavior regardless of their curing temperature.
24601217	4	47	theme	drug	874:877	arg1	model					879:883	loaded drug model	867:883	loaded drug model	867:883	Methylene blue (MB) was used as loaded drug model.
24601217	4	47	theme	drug	874:877	arg1	Methylene					835:843	Methylene blue	835:848	Methylene blue (MB)	835:853	Methylene blue (MB) was used as loaded drug model.
24601217	1	48	dep	fast	272:275	arg1	uncontrolled					283:294	uncontrolled	283:294	uncontrolled	283:294	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	1	49	dep	loading	217:223	arg1	profiles					310:317	drug release profiles	297:317	drug release profiles	297:317	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	2	50	theme	ion-crosslinked	425:439	arg1	complexes					450:458	these metal ion-crosslinked alginate complexes	413:458	these metal ion-crosslinked alginate complexes	413:458	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	0	51	theme	crosslinked	36:46	arg1	beads					48:52	novel ionotropically crosslinked beads	15:52	novel ionotropically crosslinked beads	15:52	Preparation of novel ionotropically crosslinked beads based on alginate-terephthalic acid composites as potential controlled release matrices.
24601217	2	52	dep	crosslinking	542:553	arg1	aid					555:557	aid	555:557	aid	555:557	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	1	53	theme	biorelevant	244:254	arg1	media					256:260	biorelevant media	244:260	biorelevant media	244:260	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	2	54	theme	acid	498:501	arg1	incorporation					468:480	the incorporation	464:480	the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid	464:557	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	8	55	link	zinc-crosslinked	1405:1420	arg1	beads					1445:1449	zinc-crosslinked alginate-terephthalate beads	1405:1449	zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C.	1405:1521	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	1	56	link	crosslinked	158:168	arg1	beads					179:183	Ionotropically crosslinked alginate beads	143:183	Ionotropically crosslinked alginate beads	143:183	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	8	57	theme	degrees	1512:1518	arg1	C.					1520:1521	40 degrees C.	1509:1521	40 degrees C.	1509:1521	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	3	58	theme	acid	602:605	arg1	effect					579:584	the effect	575:584	the effect of terephthalic acid	575:605	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	2	59	theme	terephthalic	485:496	arg1	acid					498:501	terephthalic acid	485:501	terephthalic acid which is proposed to act as a bridging crosslinking aid	485:557	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	2	59	theme	terephthalic	485:496	arg1	crosslinking					542:553	a bridging crosslinking	531:553	a bridging crosslinking aid	531:557	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	3	60	dep	ions	713:716	arg1	calcium					725:731	calcium	725:731	calcium	725:731	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	60	dep	ions	713:716	arg1	zinc					719:722	zinc	719:722	zinc	719:722	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	60	dep	ions	713:716	arg1	ions					713:716	curing cross-linking metal ions	686:716	curing cross-linking metal ions (zinc, calcium and aluminum)	686:745	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	60	dep	ions	713:716	arg1	aluminum					737:744	aluminum	737:744	aluminum	737:744	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	2	61	link	ion-crosslinked	425:439	arg1	complexes					450:458	these metal ion-crosslinked alginate complexes	413:458	these metal ion-crosslinked alginate complexes	413:458	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	5	62	theme	scanning	989:996	arg1	calorimetry					998:1008	differential scanning calorimetry	976:1008	differential scanning calorimetry	976:1008	The resulting beads were chemically and physically probed using infrared spectroscopy and differential scanning calorimetry.
24601217	8	63	theme	curing	1650:1655	arg1	temperatures					1657:1668	their curing temperatures	1644:1668	their curing temperatures	1644:1668	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	6	64	theme	accessed	1149:1156	arg1	profiles					1177:1184	accessed controlled release profiles	1149:1184	accessed controlled release profiles	1149:1184	Interestingly, all prepared alginate-terephthalate beads (i.e., cured with zinc, calcium and aluminum) illustrated higher MB loadings and accessed controlled release profiles compared to their corresponding terephthalate-free alginate counterparts.
24601217	3	65	theme	degrees	671:677	arg1	C					679:679	4, 25 and 40 degrees C	658:679	4, 25 and 40 degrees C	658:679	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	65	theme	degrees	671:677	arg1	temperature					645:655	temperature	645:655	temperature (4, 25 and 40 degrees C)	645:680	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	1	66	from	stability	231:239	arg1	media					256:260	biorelevant media	244:260	biorelevant media	244:260	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	1	67	theme	crosslinked	158:168	arg1	beads					179:183	Ionotropically crosslinked alginate beads	143:183	Ionotropically crosslinked alginate beads	143:183	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	3	68	theme	release	817:823	arg1	profiles					825:832	release profiles	817:832	release profiles	817:832	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	8	69	theme	alginate-terephthalate	1553:1574	arg1	beads					1576:1580	aluminum-crosslinked alginate-terephthalate beads	1532:1580	aluminum-crosslinked alginate-terephthalate beads	1532:1580	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	6	70	theme	release	1169:1175	arg1	profiles					1177:1184	accessed controlled release profiles	1149:1184	accessed controlled release profiles	1149:1184	Interestingly, all prepared alginate-terephthalate beads (i.e., cured with zinc, calcium and aluminum) illustrated higher MB loadings and accessed controlled release profiles compared to their corresponding terephthalate-free alginate counterparts.
24601217	1	71	from	loading	217:223	arg1	media					256:260	biorelevant media	244:260	biorelevant media	244:260	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	1	72	theme	alginate	170:177	arg1	beads					179:183	Ionotropically crosslinked alginate beads	143:183	Ionotropically crosslinked alginate beads	143:183	Ionotropically crosslinked alginate beads suffer generally from poor drug loading, poor stability in biorelevant media and hence fast (i.e. uncontrolled) drug release profiles.
24601217	3	73	theme	drug	800:803	arg1	loading					805:811	drug loading	800:811	drug loading	800:811	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	3	74	theme	resulting	777:785	arg1	beads					787:791	the resulting beads	773:791	the resulting beads	773:791	In addition to the effect of terephthalic acid, we investigated the effect of curing temperature (4, 25 and 40 degrees C) and curing cross-linking metal ions (zinc, calcium and aluminum) on the characteristics of the resulting beads, i.e., drug loading and release profiles.
24601217	0	75	theme	acid	85:88	arg1	composites					90:99	alginate-terephthalic acid composites	63:99	alginate-terephthalic acid composites as potential controlled release matrices	63:140	Preparation of novel ionotropically crosslinked beads based on alginate-terephthalic acid composites as potential controlled release matrices.
24601217	2	76	theme	metal	419:423	arg1	complexes					450:458	these metal ion-crosslinked alginate complexes	413:458	these metal ion-crosslinked alginate complexes	413:458	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	6	77	theme	controlled	1158:1167	arg1	profiles					1177:1184	accessed controlled release profiles	1149:1184	accessed controlled release profiles	1149:1184	Interestingly, all prepared alginate-terephthalate beads (i.e., cured with zinc, calcium and aluminum) illustrated higher MB loadings and accessed controlled release profiles compared to their corresponding terephthalate-free alginate counterparts.
24601217	2	78	theme	complexes	450:458	arg1	stability					400:408	the stability	396:408	the stability of these metal ion-crosslinked alginate complexes	396:458	Therefore, the present work aims at addressing these drawbacks by enhancing the stability of these metal ion-crosslinked alginate complexes via the incorporation of terephthalic acid which is proposed to act as a bridging crosslinking aid.
24601217	8	79	theme	enteric-like	1460:1471	arg1	profiles					1481:1488	enteric-like release profiles	1460:1488	enteric-like release profiles	1460:1488	On the other hand, zinc-crosslinked alginate-terephthalate beads accessed enteric-like release profiles when only cured at 40 degrees C. However, aluminum-crosslinked alginate-terephthalate beads yielded interesting zero order release profiles regardless of their curing temperatures.
24601217	6	80	theme	alginate-terephthalate	1039:1060	arg1	beads					1062:1066	all prepared alginate-terephthalate beads	1026:1066	all prepared alginate-terephthalate beads	1026:1066	Interestingly, all prepared alginate-terephthalate beads (i.e., cured with zinc, calcium and aluminum) illustrated higher MB loadings and accessed controlled release profiles compared to their corresponding terephthalate-free alginate counterparts.
24601217	4	81	used	used	859:862	arg2	model					879:883	loaded drug model	867:883	loaded drug model	867:883	Methylene blue (MB) was used as loaded drug model.
24601217	4	81	used	used	859:862	arg2	Methylene					835:843	Methylene blue	835:848	Methylene blue (MB)	835:853	Methylene blue (MB) was used as loaded drug model.
24601217	4	81	used	used	859:862	arg2	MB					851:852	MB	851:852	MB	851:852	Methylene blue (MB) was used as loaded drug model.
24601217	0	82	theme	alginate-terephthalic	63:83	arg1	composites					90:99	alginate-terephthalic acid composites	63:99	alginate-terephthalic acid composites as potential controlled release matrices	63:140	Preparation of novel ionotropically crosslinked beads based on alginate-terephthalic acid composites as potential controlled release matrices.
24601217	5	83	theme	infrared	950:957	arg1	spectroscopy					959:970	infrared spectroscopy	950:970	infrared spectroscopy	950:970	The resulting beads were chemically and physically probed using infrared spectroscopy and differential scanning calorimetry.
24601217	7	84	theme	Calcium-crosslinked	1260:1278	arg1	beads					1303:1307	Calcium-crosslinked alginate-terephthalate beads	1260:1307	Calcium-crosslinked alginate-terephthalate beads	1260:1307	Calcium-crosslinked alginate-terephthalate beads illustrated enteric release behavior regardless of their curing temperature.
28295383	9	0	theme	explosion	956:964	arg1	process					966:972	the steam explosion process	946:972	the steam explosion process	946:972	Following the steam explosion process almost all of the pectic hydrocolloids were recoverable with a water wash.
28295383	12	1	theme	valuable	1468:1475	arg1	coproducts					1477:1486	valuable coproducts	1468:1486	valuable coproducts	1468:1486	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	6	2	theme	fruit	730:734	arg1	composition					711:721	The sugar composition	701:721	The sugar composition of raw fruit and steam-exploded biomass	701:761	The sugar composition of raw fruit and steam-exploded biomass was determined.
28295383	8	3	theme	g	887:887	arg1	kg-1					889:892	78 g kg-1	884:892	78 g kg-1 of the dry material in the dropped fruit	884:933	Pectic hydrocolloids comprised 78 g kg-1 of the dry material in the dropped fruit.
28295383	4	4	theme	explosion	580:588	arg1	process					590:596	our newly developed pilot scale continuous steam explosion process	531:596	our newly developed pilot scale continuous steam explosion process	531:596	Therefore, we have explored the potential of using this fruit as a feedstock in our newly developed pilot scale continuous steam explosion process.
28295383	0	5	theme	citrus	82:87	arg1	fruit					89:93	citrus fruit	82:93	citrus fruit	82:93	Recovery of pectic hydrocolloids and phenolics from huanglongbing related dropped citrus fruit.
28295383	12	6	theme	friendly	1420:1427	arg1	method					1429:1434	an enhanced, environmentally friendly method	1391:1434	an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass	1391:1506	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	5	7	theme	pilot	680:684	arg1	scale					686:690	a continuous pilot scale	667:690	a continuous pilot scale process	667:698	RESULTS Whole fruits were converted to steam-exploded biomass using a continuous pilot scale process.
28295383	4	8	theme	steam	574:578	arg1	process					590:596	our newly developed pilot scale continuous steam explosion process	531:596	our newly developed pilot scale continuous steam explosion process	531:596	Therefore, we have explored the potential of using this fruit as a feedstock in our newly developed pilot scale continuous steam explosion process.
28295383	5	9	theme	continuous	669:678	arg1	scale					686:690	a continuous pilot scale	667:690	a continuous pilot scale process	667:698	RESULTS Whole fruits were converted to steam-exploded biomass using a continuous pilot scale process.
28295383	5	10	theme	Whole	607:611	arg1	fruits					613:618	Whole fruits	607:618	RESULTS Whole fruits	599:618	RESULTS Whole fruits were converted to steam-exploded biomass using a continuous pilot scale process.
28295383	12	11	theme	pre-harvest	1349:1359	arg1	fruit					1376:1380	pre-harvest dropped citrus fruit	1349:1380	pre-harvest dropped citrus fruit	1349:1380	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	14	12	theme	public	1578:1583	arg1	domain					1585:1590	the public domain	1574:1590	the public domain in the USA	1574:1601	This article is a U.S. Government work and is in the public domain in the USA.
28295383	14	13	from	work	1559:1562	arg1	USA					1599:1601	the USA	1595:1601	the USA	1595:1601	This article is a U.S. Government work and is in the public domain in the USA.
28295383	3	14	dep	added	401:405	arg1	value					395:399	value	395:399	value	395:399	Recovering and converting this fruit to alternative value added products would benefit the citrus industry.
28295383	11	15	theme	flavanone	1193:1201	arg1	glycosides					1203:1212	the flavanone glycosides	1189:1212	the flavanone glycosides	1189:1212	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	6	16	theme	raw	726:728	arg1	fruit					730:734	raw fruit	726:734	raw fruit	726:734	The sugar composition of raw fruit and steam-exploded biomass was determined.
28295383	5	17	dep	scale	686:690	arg1	process					692:698	process	692:698	a continuous pilot scale process	667:698	RESULTS Whole fruits were converted to steam-exploded biomass using a continuous pilot scale process.
28295383	8	18	theme	material	905:912	arg1	kg-1					889:892	78 g kg-1	884:892	78 g kg-1 of the dry material in the dropped fruit	884:933	Pectic hydrocolloids comprised 78 g kg-1 of the dry material in the dropped fruit.
28295383	2	19	theme	fruit	287:291	arg1	loss					267:270	This loss	262:270	This loss of harvestable fruit	262:291	This loss of harvestable fruit is damaging to both growers and juice processors.
28295383	8	20	theme	dry	901:903	arg1	material					905:912	the dry material	897:912	the dry material	897:912	Pectic hydrocolloids comprised 78 g kg-1 of the dry material in the dropped fruit.
28295383	12	21	from	biomass	1500:1506	arg1	recovery					1456:1463	recovery	1456:1463	recovery	1456:1463	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	12	21	from	biomass	1500:1506	arg1	release					1444:1450	release	1444:1450	release	1444:1450	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	2	22	theme	harvestable	275:285	arg1	fruit					287:291	harvestable fruit	275:291	harvestable fruit	275:291	This loss of harvestable fruit is damaging to both growers and juice processors.
28295383	9	23	theme	pectic	992:997	arg1	hydrocolloids					999:1011	the pectic hydrocolloids	988:1011	the pectic hydrocolloids	988:1011	Following the steam explosion process almost all of the pectic hydrocolloids were recoverable with a water wash.
28295383	12	24	dep	release	1444:1450	arg1	the					1440:1442	the	1440:1442	the	1440:1442	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	5	25	theme	steam-exploded	638:651	arg1	biomass					653:659	steam-exploded biomass	638:659	steam-exploded biomass	638:659	RESULTS Whole fruits were converted to steam-exploded biomass using a continuous pilot scale process.
28295383	14	26	theme	Government	1548:1557	arg1	work					1559:1562	a U.S. Government work	1541:1562	a U.S. Government work	1541:1562	This article is a U.S. Government work and is in the public domain in the USA.
28295383	14	26	theme	Government	1548:1557	arg1	article					1530:1536	This article	1525:1536	This article	1525:1536	This article is a U.S. Government work and is in the public domain in the USA.
28295383	12	27	theme	enhanced	1394:1401	arg1	method					1429:1434	an enhanced, environmentally friendly method	1391:1434	an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass	1391:1506	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	6	28	theme	sugar	705:709	arg1	composition					711:721	The sugar composition	701:721	The sugar composition of raw fruit and steam-exploded biomass	701:761	The sugar composition of raw fruit and steam-exploded biomass was determined.
28295383	3	29	theme	citrus	434:439	arg1	industry					441:448	the citrus industry	430:448	the citrus industry	430:448	Recovering and converting this fruit to alternative value added products would benefit the citrus industry.
28295383	3	30	theme	added	401:405	arg1	products					407:414	alternative value added products	383:414	alternative value added products	383:414	Recovering and converting this fruit to alternative value added products would benefit the citrus industry.
28295383	4	31	theme	pilot	551:555	arg1	process					590:596	our newly developed pilot scale continuous steam explosion process	531:596	our newly developed pilot scale continuous steam explosion process	531:596	Therefore, we have explored the potential of using this fruit as a feedstock in our newly developed pilot scale continuous steam explosion process.
28295383	14	32	theme	U.S.	1543:1546	arg1	work					1559:1562	a U.S. Government work	1541:1562	a U.S. Government work	1541:1562	This article is a U.S. Government work and is in the public domain in the USA.
28295383	14	32	theme	U.S.	1543:1546	arg1	article					1530:1536	This article	1525:1536	This article	1525:1536	This article is a U.S. Government work and is in the public domain in the USA.
28295383	1	33	theme	BACKGROUND	96:105	arg1	drop					132:135	BACKGROUND Citrus pre-harvest fruit drop	96:135	BACKGROUND Citrus pre-harvest fruit drop	96:135	BACKGROUND Citrus pre-harvest fruit drop, caused by huanglongbing infection, has increased dramatically concomitant with declining tree health and crop harvest size.
28295383	12	34	theme	continuous	1319:1328	arg1	explosion					1336:1344	The continuous steam explosion	1315:1344	The continuous steam explosion of pre-harvest dropped citrus fruit	1315:1380	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	0	35	theme	hydrocolloids	19:31	arg1	Recovery					0:7	Recovery	0:7	Recovery of pectic hydrocolloids and phenolics from huanglongbing related	0:72	Recovery of pectic hydrocolloids and phenolics from huanglongbing related dropped citrus fruit.
28295383	4	36	theme	developed	541:549	arg1	process					590:596	our newly developed pilot scale continuous steam explosion process	531:596	our newly developed pilot scale continuous steam explosion process	531:596	Therefore, we have explored the potential of using this fruit as a feedstock in our newly developed pilot scale continuous steam explosion process.
28295383	11	37	theme	limonoids	1226:1234	arg1	limonoids					1226:1234	the limonoids	1222:1234	the limonoids	1222:1234	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	37	theme	limonoids	1226:1234	arg1	flavones					1172:1179	the polymethoxylated flavones	1151:1179	the polymethoxylated flavones	1151:1179	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	37	theme	limonoids	1226:1234	arg1	hydroxycinnamates					1255:1271	hydroxycinnamates	1255:1271	hydroxycinnamates	1255:1271	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	37	theme	limonoids	1226:1234	arg1	glycosides					1203:1212	the flavanone glycosides	1189:1212	the flavanone glycosides	1189:1212	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	37	theme	limonoids	1226:1234	arg1	%					1146:1146	approximately 40%	1130:1146	approximately 40% of the polymethoxylated flavones	1130:1179	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	37	theme	limonoids	1226:1234	arg1	%					1217:1217	85%	1215:1217	85% of the limonoids	1215:1234	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	37	theme	limonoids	1226:1234	arg1	%					1184:1184	10%	1182:1184	10% of the flavanone glycosides	1182:1212	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	37	theme	limonoids	1226:1234	arg1	%					1250:1250	almost 100%	1240:1250	almost 100% of hydroxycinnamates	1240:1271	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	12	38	theme	fruit	1376:1380	arg1	explosion					1336:1344	The continuous steam explosion	1315:1344	The continuous steam explosion of pre-harvest dropped citrus fruit	1315:1380	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	1	39	theme	Citrus	107:112	arg1	drop					132:135	BACKGROUND Citrus pre-harvest fruit drop	96:135	BACKGROUND Citrus pre-harvest fruit drop	96:135	BACKGROUND Citrus pre-harvest fruit drop, caused by huanglongbing infection, has increased dramatically concomitant with declining tree health and crop harvest size.
28295383	8	40	theme	dropped	921:927	arg1	fruit					929:933	the dropped fruit	917:933	the dropped fruit	917:933	Pectic hydrocolloids comprised 78 g kg-1 of the dry material in the dropped fruit.
28295383	3	41	theme	alternative	383:393	arg1	products					407:414	alternative value added products	383:414	alternative value added products	383:414	Recovering and converting this fruit to alternative value added products would benefit the citrus industry.
28295383	4	42	theme	continuous	563:572	arg1	process					590:596	our newly developed pilot scale continuous steam explosion process	531:596	our newly developed pilot scale continuous steam explosion process	531:596	Therefore, we have explored the potential of using this fruit as a feedstock in our newly developed pilot scale continuous steam explosion process.
28295383	9	43	with	recoverable	1018:1028	arg1	wash					1043:1046	a water wash	1035:1046	a water wash	1035:1046	Following the steam explosion process almost all of the pectic hydrocolloids were recoverable with a water wash.
28295383	1	44	theme	pre-harvest	114:124	arg1	drop					132:135	BACKGROUND Citrus pre-harvest fruit drop	96:135	BACKGROUND Citrus pre-harvest fruit drop	96:135	BACKGROUND Citrus pre-harvest fruit drop, caused by huanglongbing infection, has increased dramatically concomitant with declining tree health and crop harvest size.
28295383	1	45	theme	declining	217:225	arg1	health					232:237	declining tree health	217:237	declining tree health	217:237	BACKGROUND Citrus pre-harvest fruit drop, caused by huanglongbing infection, has increased dramatically concomitant with declining tree health and crop harvest size.
28295383	0	46	theme	phenolics	37:45	arg1	Recovery					0:7	Recovery	0:7	Recovery of pectic hydrocolloids and phenolics from huanglongbing related	0:72	Recovery of pectic hydrocolloids and phenolics from huanglongbing related dropped citrus fruit.
28295383	4	47	theme	scale	557:561	arg1	process					590:596	our newly developed pilot scale continuous steam explosion process	531:596	our newly developed pilot scale continuous steam explosion process	531:596	Therefore, we have explored the potential of using this fruit as a feedstock in our newly developed pilot scale continuous steam explosion process.
28295383	12	48	theme	citrus	1369:1374	arg1	fruit					1376:1380	pre-harvest dropped citrus fruit	1349:1380	pre-harvest dropped citrus fruit	1349:1380	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	1	49	theme	tree	227:230	arg1	health					232:237	declining tree health	217:237	declining tree health	217:237	BACKGROUND Citrus pre-harvest fruit drop, caused by huanglongbing infection, has increased dramatically concomitant with declining tree health and crop harvest size.
28295383	12	50	theme	wasted	1493:1498	arg1	biomass					1500:1506	wasted biomass	1493:1506	wasted biomass	1493:1506	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	12	51	theme	dropped	1361:1367	arg1	fruit					1376:1380	pre-harvest dropped citrus fruit	1349:1380	pre-harvest dropped citrus fruit	1349:1380	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	9	52	theme	water	1037:1041	arg1	wash					1043:1046	a water wash	1035:1046	a water wash	1035:1046	Following the steam explosion process almost all of the pectic hydrocolloids were recoverable with a water wash.
28295383	7	53	theme	phenolic	814:821	arg1	compounds					823:831	phenolic compounds	814:831	phenolic compounds	814:831	Recovered pectic hydrocolloids and phenolic compounds were characterized.
28295383	0	54	from	related	66:72	arg1	Recovery					0:7	Recovery	0:7	Recovery of pectic hydrocolloids and phenolics from huanglongbing related	0:72	Recovery of pectic hydrocolloids and phenolics from huanglongbing related dropped citrus fruit.
28295383	1	55	theme	fruit	126:130	arg1	drop					132:135	BACKGROUND Citrus pre-harvest fruit drop	96:135	BACKGROUND Citrus pre-harvest fruit drop	96:135	BACKGROUND Citrus pre-harvest fruit drop, caused by huanglongbing infection, has increased dramatically concomitant with declining tree health and crop harvest size.
28295383	4	56	from	feedstock	518:526	arg1	process					590:596	our newly developed pilot scale continuous steam explosion process	531:596	our newly developed pilot scale continuous steam explosion process	531:596	Therefore, we have explored the potential of using this fruit as a feedstock in our newly developed pilot scale continuous steam explosion process.
28295383	7	57	theme	pectic	789:794	arg1	hydrocolloids					796:808	pectic hydrocolloids	789:808	pectic hydrocolloids	789:808	Recovered pectic hydrocolloids and phenolic compounds were characterized.
28295383	1	58	with	concomitant	200:210	arg1	size					256:259	crop harvest size	243:259	crop harvest size	243:259	BACKGROUND Citrus pre-harvest fruit drop, caused by huanglongbing infection, has increased dramatically concomitant with declining tree health and crop harvest size.
28295383	1	58	with	concomitant	200:210	arg1	health					232:237	declining tree health	217:237	declining tree health	217:237	BACKGROUND Citrus pre-harvest fruit drop, caused by huanglongbing infection, has increased dramatically concomitant with declining tree health and crop harvest size.
28295383	8	59	from	kg-1	889:892	arg1	fruit					929:933	the dropped fruit	917:933	the dropped fruit	917:933	Pectic hydrocolloids comprised 78 g kg-1 of the dry material in the dropped fruit.
28295383	11	60	theme	hydroxycinnamates	1255:1271	arg1	limonoids					1226:1234	the limonoids	1222:1234	the limonoids	1222:1234	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	60	theme	hydroxycinnamates	1255:1271	arg1	flavones					1172:1179	the polymethoxylated flavones	1151:1179	the polymethoxylated flavones	1151:1179	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	60	theme	hydroxycinnamates	1255:1271	arg1	hydroxycinnamates					1255:1271	hydroxycinnamates	1255:1271	hydroxycinnamates	1255:1271	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	60	theme	hydroxycinnamates	1255:1271	arg1	glycosides					1203:1212	the flavanone glycosides	1189:1212	the flavanone glycosides	1189:1212	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	60	theme	hydroxycinnamates	1255:1271	arg1	%					1146:1146	approximately 40%	1130:1146	approximately 40% of the polymethoxylated flavones	1130:1179	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	60	theme	hydroxycinnamates	1255:1271	arg1	%					1217:1217	85%	1215:1217	85% of the limonoids	1215:1234	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	60	theme	hydroxycinnamates	1255:1271	arg1	%					1184:1184	10%	1182:1184	10% of the flavanone glycosides	1182:1212	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	60	theme	hydroxycinnamates	1255:1271	arg1	%					1250:1250	almost 100%	1240:1250	almost 100% of hydroxycinnamates	1240:1271	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	61	theme	flavones	1172:1179	arg1	limonoids					1226:1234	the limonoids	1222:1234	the limonoids	1222:1234	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	61	theme	flavones	1172:1179	arg1	flavones					1172:1179	the polymethoxylated flavones	1151:1179	the polymethoxylated flavones	1151:1179	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	61	theme	flavones	1172:1179	arg1	hydroxycinnamates					1255:1271	hydroxycinnamates	1255:1271	hydroxycinnamates	1255:1271	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	61	theme	flavones	1172:1179	arg1	glycosides					1203:1212	the flavanone glycosides	1189:1212	the flavanone glycosides	1189:1212	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	61	theme	flavones	1172:1179	arg1	%					1146:1146	approximately 40%	1130:1146	approximately 40% of the polymethoxylated flavones	1130:1179	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	61	theme	flavones	1172:1179	arg1	%					1217:1217	85%	1215:1217	85% of the limonoids	1215:1234	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	61	theme	flavones	1172:1179	arg1	%					1184:1184	10%	1182:1184	10% of the flavanone glycosides	1182:1212	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	61	theme	flavones	1172:1179	arg1	%					1250:1250	almost 100%	1240:1250	almost 100% of hydroxycinnamates	1240:1271	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	14	62	from	domain	1585:1590	arg1	USA					1599:1601	the USA	1595:1601	the USA	1595:1601	This article is a U.S. Government work and is in the public domain in the USA.
28295383	12	63	dep	enhanced	1394:1401	arg1	environmentally					1404:1418	environmentally	1404:1418	environmentally	1404:1418	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	8	64	theme	Pectic	853:858	arg1	hydrocolloids					860:872	Pectic hydrocolloids	853:872	Pectic hydrocolloids	853:872	Pectic hydrocolloids comprised 78 g kg-1 of the dry material in the dropped fruit.
28295383	12	65	theme	steam	1330:1334	arg1	explosion					1336:1344	The continuous steam explosion	1315:1344	The continuous steam explosion of pre-harvest dropped citrus fruit	1315:1380	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	6	66	theme	biomass	755:761	arg1	composition					711:721	The sugar composition	701:721	The sugar composition of raw fruit and steam-exploded biomass	701:761	The sugar composition of raw fruit and steam-exploded biomass was determined.
28295383	2	67	theme	juice	325:329	arg1	processors					331:340	juice processors	325:340	juice processors	325:340	This loss of harvestable fruit is damaging to both growers and juice processors.
28295383	5	68	dep	RESULTS	599:605	arg1	fruits					613:618	Whole fruits	607:618	RESULTS Whole fruits	599:618	RESULTS Whole fruits were converted to steam-exploded biomass using a continuous pilot scale process.
28295383	1	69	theme	crop	243:246	arg1	size					256:259	crop harvest size	243:259	crop harvest size	243:259	BACKGROUND Citrus pre-harvest fruit drop, caused by huanglongbing infection, has increased dramatically concomitant with declining tree health and crop harvest size.
28295383	7	70	theme	Recovered	779:787	arg1	hydrocolloids					796:808	pectic hydrocolloids	789:808	pectic hydrocolloids	789:808	Recovered pectic hydrocolloids and phenolic compounds were characterized.
28295383	11	71	theme	polymethoxylated	1155:1170	arg1	flavones					1172:1179	the polymethoxylated flavones	1151:1179	the polymethoxylated flavones	1151:1179	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	6	72	theme	steam-exploded	740:753	arg1	biomass					755:761	steam-exploded biomass	740:761	steam-exploded biomass	740:761	The sugar composition of raw fruit and steam-exploded biomass was determined.
28295383	12	73	dep	CONCLUSION	1304:1313	arg1	provides					1382:1389	provides	1382:1389	provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass	1382:1506	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	1	74	theme	harvest	248:254	arg1	size					256:259	crop harvest size	243:259	crop harvest size	243:259	BACKGROUND Citrus pre-harvest fruit drop, caused by huanglongbing infection, has increased dramatically concomitant with declining tree health and crop harvest size.
28295383	9	75	theme	steam	950:954	arg1	process					966:972	the steam explosion process	946:972	the steam explosion process	946:972	Following the steam explosion process almost all of the pectic hydrocolloids were recoverable with a water wash.
28295383	12	76	theme	coproducts	1477:1486	arg1	recovery					1456:1463	recovery	1456:1463	recovery	1456:1463	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	12	76	theme	coproducts	1477:1486	arg1	release					1444:1450	release	1444:1450	release	1444:1450	CONCLUSION The continuous steam explosion of pre-harvest dropped citrus fruit provides an enhanced, environmentally friendly method for the release and recovery of valuable coproducts from wasted biomass.
28295383	0	77	theme	pectic	12:17	arg1	hydrocolloids					19:31	pectic hydrocolloids	12:31	pectic hydrocolloids	12:31	Recovery of pectic hydrocolloids and phenolics from huanglongbing related dropped citrus fruit.
28295383	1	78	theme	huanglongbing	148:160	arg1	infection					162:170	huanglongbing infection	148:170	huanglongbing infection	148:170	BACKGROUND Citrus pre-harvest fruit drop, caused by huanglongbing infection, has increased dramatically concomitant with declining tree health and crop harvest size.
28295383	11	79	theme	glycosides	1203:1212	arg1	limonoids					1226:1234	the limonoids	1222:1234	the limonoids	1222:1234	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	79	theme	glycosides	1203:1212	arg1	flavones					1172:1179	the polymethoxylated flavones	1151:1179	the polymethoxylated flavones	1151:1179	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	79	theme	glycosides	1203:1212	arg1	hydroxycinnamates					1255:1271	hydroxycinnamates	1255:1271	hydroxycinnamates	1255:1271	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	79	theme	glycosides	1203:1212	arg1	glycosides					1203:1212	the flavanone glycosides	1189:1212	the flavanone glycosides	1189:1212	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	79	theme	glycosides	1203:1212	arg1	%					1146:1146	approximately 40%	1130:1146	approximately 40% of the polymethoxylated flavones	1130:1179	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	79	theme	glycosides	1203:1212	arg1	%					1217:1217	85%	1215:1217	85% of the limonoids	1215:1234	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	79	theme	glycosides	1203:1212	arg1	%					1184:1184	10%	1182:1184	10% of the flavanone glycosides	1182:1212	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
28295383	11	79	theme	glycosides	1203:1212	arg1	%					1250:1250	almost 100%	1240:1250	almost 100% of hydroxycinnamates	1240:1271	Additionally, approximately 40% of the polymethoxylated flavones, 10% of the flavanone glycosides, 85% of the limonoids and almost 100% of hydroxycinnamates were simultaneously recovered.
27492557	7	0	theme	films	1151:1155	arg1	strength					1092:1099	mechanical strength	1081:1099	mechanical strength	1081:1099	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	7	0	theme	films	1151:1155	arg1	vapor					1112:1116	water vapor	1106:1116	water vapor	1106:1116	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	7	0	theme	films	1151:1155	arg1	stability					1070:1078	thermal stability	1062:1078	thermal stability	1062:1078	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	1	1	theme	antimicrobial	314:326	arg1	films					338:342	pectin-based antimicrobial composite films	301:342	pectin-based antimicrobial composite films	301:342	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	7	2	theme	thermal	1062:1068	arg1	stability					1070:1078	thermal stability	1062:1078	thermal stability	1062:1078	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	7	3	theme	pectin	949:954	arg1	film					956:959	the pectin film	945:959	the pectin film	945:959	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	3	4	theme	pectin/AgNPs	466:477	arg1	film					489:492	The pectin/AgNPs composite film	462:492	The pectin/AgNPs composite film	462:492	The pectin/AgNPs composite film exhibited characteristic absorption peak of AgNPs at 480nm.
27492557	6	5	theme	little	865:870	arg1	decrease					872:879	a little decrease	863:879	a little decrease in the transparency	863:899	UV-light barrier property of the pectin film increased significantly with a little decrease in the transparency.
27492557	4	6	theme	surface	558:564	arg1	color					566:570	The surface color and light transmittance	554:594	color	566:570	The surface color and light transmittance of the pectin films were greatly influenced by the addition of AgNPs.
27492557	2	7	with	spherical	360:368	arg1	size					388:391	the size	384:391	the size in the range of 20-80nm	384:415	The AgNPs were spherical in shape with the size in the range of 20-80nm and showed the absorption peak around 500nm.
27492557	1	8	theme	composite	328:336	arg1	films					338:342	pectin-based antimicrobial composite films	301:342	pectin-based antimicrobial composite films	301:342	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	3	9	theme	composite	479:487	arg1	film					489:492	The pectin/AgNPs composite film	462:492	The pectin/AgNPs composite film	462:492	The pectin/AgNPs composite film exhibited characteristic absorption peak of AgNPs at 480nm.
27492557	1	10	theme	stabilizing	239:249	arg1	extract					177:183	an aqueous extract	166:183	an aqueous extract of Caesalpinia mimosoides Lamk (CMLE)	166:221	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	1	10	theme	stabilizing	239:249	arg1	agents					251:256	reducing and stabilizing agents	226:256	reducing and stabilizing agents	226:256	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	1	11	theme	films	338:342	arg1	preparation					286:296	the preparation	282:296	the preparation of pectin-based antimicrobial composite films	282:342	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	7	12	theme	pectin	1144:1149	arg1	films					1151:1155	the pectin films	1140:1155	the pectin films	1140:1155	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	1	13	used	used	273:276	arg2	they					263:266	they	263:266	they	263:266	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	7	14	theme	CMLE	985:988	arg1	incorporation					968:980	the incorporation	964:980	the incorporation of CMLE and AgNPs	964:998	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	7	15	from	changes	934:940	arg1	film					956:959	the pectin film	945:959	the pectin film	945:959	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	8	16	theme	Listeria	1306:1313	arg1	bacteria					1275:1282	food-borne pathogenic bacteria	1253:1282	food-borne pathogenic bacteria	1253:1282	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	8	16	theme	Listeria	1306:1313	arg1	monocytogenes					1315:1327	Listeria monocytogenes	1306:1327	Listeria monocytogenes	1306:1327	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	7	17	theme	water	1106:1110	arg1	vapor					1112:1116	water vapor	1106:1116	water vapor	1106:1116	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	4	18	theme	pectin	603:608	arg1	films					610:614	the pectin films	599:614	the pectin films	599:614	The surface color and light transmittance of the pectin films were greatly influenced by the addition of AgNPs.
27492557	3	19	theme	characteristic	504:517	arg1	peak					530:533	characteristic absorption peak	504:533	characteristic absorption peak of AgNPs at 480nm	504:551	The pectin/AgNPs composite film exhibited characteristic absorption peak of AgNPs at 480nm.
27492557	5	20	theme	films	743:747	arg1	yellowness					725:734	yellowness	725:734	yellowness	725:734	The lightness of the films decreased, however, redness and yellowness of the films increased after incorporation of AgNPs.
27492557	5	20	theme	films	743:747	arg1	redness					713:719	redness	713:719	redness	713:719	The lightness of the films decreased, however, redness and yellowness of the films increased after incorporation of AgNPs.
27492557	7	21	theme	mechanical	1081:1090	arg1	strength					1092:1099	mechanical strength	1081:1099	mechanical strength	1081:1099	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	3	22	theme	absorption	519:528	arg1	peak					530:533	characteristic absorption peak	504:533	characteristic absorption peak of AgNPs at 480nm	504:551	The pectin/AgNPs composite film exhibited characteristic absorption peak of AgNPs at 480nm.
27492557	1	23	theme	aqueous	169:175	arg1	extract					177:183	an aqueous extract	166:183	an aqueous extract of Caesalpinia mimosoides Lamk (CMLE)	166:221	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	1	23	theme	aqueous	169:175	arg1	agents					251:256	reducing and stabilizing agents	226:256	reducing and stabilizing agents	226:256	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	7	24	dep	stability	1070:1078	arg1	properties					1126:1135	barrier properties	1118:1135	barrier properties	1118:1135	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	1	25	theme	Silver	97:102	arg1	nanoparticles					104:116	Silver nanoparticles	97:116	Silver nanoparticles (AgNPs)	97:124	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	1	25	theme	Silver	97:102	arg1	AgNPs					119:123	AgNPs	119:123	AgNPs	119:123	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	2	26	theme	20-80nm	409:415	arg1	range					400:404	the range	396:404	the range of 20-80nm	396:415	The AgNPs were spherical in shape with the size in the range of 20-80nm and showed the absorption peak around 500nm.
27492557	8	27	theme	nanocomposite	1185:1197	arg1	films					1199:1203	The pectin/AgNPs nanocomposite films	1168:1203	The pectin/AgNPs nanocomposite films	1168:1203	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	7	28	theme	film	1038:1041	arg1	strength					1092:1099	mechanical strength	1081:1099	mechanical strength	1081:1099	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	7	28	theme	film	1038:1041	arg1	properties					1043:1052	the film properties	1034:1052	the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films	1034:1155	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	7	28	theme	film	1038:1041	arg1	vapor					1112:1116	water vapor	1106:1116	water vapor	1106:1116	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	7	28	theme	film	1038:1041	arg1	stability					1070:1078	thermal stability	1062:1078	thermal stability	1062:1078	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	8	29	theme	strong	1215:1220	arg1	activity					1236:1243	strong antibacterial activity	1215:1243	strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes	1215:1327	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	5	30	theme	AgNPs	782:786	arg1	incorporation					765:777	incorporation	765:777	incorporation of AgNPs	765:786	The lightness of the films decreased, however, redness and yellowness of the films increased after incorporation of AgNPs.
27492557	7	31	theme	FTIR	1020:1023	arg1	results					1025:1031	the FTIR results	1016:1031	the FTIR results	1016:1031	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	7	32	theme	structural	923:932	arg1	changes					934:940	no structural changes	920:940	no structural changes in the pectin film by the incorporation of CMLE and AgNPs	920:998	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	3	33	theme	AgNPs	538:542	arg1	peak					530:533	characteristic absorption peak	504:533	characteristic absorption peak of AgNPs at 480nm	504:551	The pectin/AgNPs composite film exhibited characteristic absorption peak of AgNPs at 480nm.
27492557	1	34	theme	Caesalpinia	188:198	arg1	mimosoides					200:209	Caesalpinia mimosoides Lamk (CMLE)	188:221	Caesalpinia mimosoides Lamk (CMLE)	188:221	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	0	35	theme	pectin/silver	15:27	arg1	Preparation					0:10	Preparation	0:10	Preparation of pectin/silver	0:27	Preparation of pectin/silver nanoparticles composite films with UV-light barrier and properties.
27492557	8	36	theme	antibacterial	1222:1234	arg1	activity					1236:1243	strong antibacterial activity	1215:1243	strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes	1215:1327	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	1	37	theme	mimosoides	200:209	arg1	extract					177:183	an aqueous extract	166:183	an aqueous extract of Caesalpinia mimosoides Lamk (CMLE)	166:221	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	1	37	theme	mimosoides	200:209	arg1	agents					251:256	reducing and stabilizing agents	226:256	reducing and stabilizing agents	226:256	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	6	38	theme	film	829:832	arg1	property					806:813	UV-light barrier property	789:813	UV-light barrier property of the pectin film	789:832	UV-light barrier property of the pectin film increased significantly with a little decrease in the transparency.
27492557	2	39	theme	absorption	432:441	arg1	peak					443:446	the absorption peak	428:446	the absorption peak around 500nm	428:459	The AgNPs were spherical in shape with the size in the range of 20-80nm and showed the absorption peak around 500nm.
27492557	0	40	theme	composite	43:51	arg1	films					53:57	composite films	43:57	composite films with UV-light barrier and properties	43:94	Preparation of pectin/silver nanoparticles composite films with UV-light barrier and properties.
27492557	6	41	theme	pectin	822:827	arg1	film					829:832	the pectin film	818:832	the pectin film	818:832	UV-light barrier property of the pectin film increased significantly with a little decrease in the transparency.
27492557	0	42	theme	UV-light	64:71	arg1	barrier					73:79	UV-light barrier	64:79	UV-light barrier	64:79	Preparation of pectin/silver nanoparticles composite films with UV-light barrier and properties.
27492557	2	43	from	spherical	360:368	arg1	shape					373:377	shape	373:377	shape	373:377	The AgNPs were spherical in shape with the size in the range of 20-80nm and showed the absorption peak around 500nm.
27492557	8	44	theme	pathogenic	1264:1273	arg1	bacteria					1275:1282	food-borne pathogenic bacteria	1253:1282	food-borne pathogenic bacteria	1253:1282	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	8	44	theme	pathogenic	1264:1273	arg1	coli					1297:1300	Escherichia coli	1285:1300	Escherichia coli	1285:1300	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	8	44	theme	pathogenic	1264:1273	arg1	monocytogenes					1315:1327	Listeria monocytogenes	1306:1327	Listeria monocytogenes	1306:1327	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	5	45	theme	films	687:691	arg1	lightness					670:678	The lightness	666:678	The lightness of the films	666:691	The lightness of the films decreased, however, redness and yellowness of the films increased after incorporation of AgNPs.
27492557	8	46	theme	food-borne	1253:1262	arg1	bacteria					1275:1282	food-borne pathogenic bacteria	1253:1282	food-borne pathogenic bacteria	1253:1282	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	8	46	theme	food-borne	1253:1262	arg1	coli					1297:1300	Escherichia coli	1285:1300	Escherichia coli	1285:1300	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	8	46	theme	food-borne	1253:1262	arg1	monocytogenes					1315:1327	Listeria monocytogenes	1306:1327	Listeria monocytogenes	1306:1327	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	2	47	from	shape	373:377	arg1	spherical					360:368	spherical	360:368	spherical	360:368	The AgNPs were spherical in shape with the size in the range of 20-80nm and showed the absorption peak around 500nm.
27492557	2	47	from	shape	373:377	arg1	AgNPs					349:353	The AgNPs	345:353	The AgNPs	345:353	The AgNPs were spherical in shape with the size in the range of 20-80nm and showed the absorption peak around 500nm.
27492557	7	48	theme	AgNPs	994:998	arg1	incorporation					968:980	the incorporation	964:980	the incorporation of CMLE and AgNPs	964:998	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	6	49	theme	barrier	798:804	arg1	property					806:813	UV-light barrier property	789:813	UV-light barrier property of the pectin film	789:832	UV-light barrier property of the pectin film increased significantly with a little decrease in the transparency.
27492557	2	50	from	size	388:391	arg1	range					400:404	the range	396:404	the range of 20-80nm	396:415	The AgNPs were spherical in shape with the size in the range of 20-80nm and showed the absorption peak around 500nm.
27492557	3	51	from	480nm	547:551	arg1	peak					530:533	characteristic absorption peak	504:533	characteristic absorption peak of AgNPs at 480nm	504:551	The pectin/AgNPs composite film exhibited characteristic absorption peak of AgNPs at 480nm.
27492557	4	52	theme	light	576:580	arg1	transmittance					582:594	The surface color and light transmittance	554:594	transmittance	582:594	The surface color and light transmittance of the pectin films were greatly influenced by the addition of AgNPs.
27492557	6	53	theme	UV-light	789:796	arg1	property					806:813	UV-light barrier property	789:813	UV-light barrier property of the pectin film	789:832	UV-light barrier property of the pectin film increased significantly with a little decrease in the transparency.
27492557	4	54	theme	films	610:614	arg1	color					566:570	The surface color and light transmittance	554:594	color	566:570	The surface color and light transmittance of the pectin films were greatly influenced by the addition of AgNPs.
27492557	4	54	theme	films	610:614	arg1	transmittance					582:594	The surface color and light transmittance	554:594	transmittance	582:594	The surface color and light transmittance of the pectin films were greatly influenced by the addition of AgNPs.
27492557	4	55	theme	AgNPs	659:663	arg1	addition					647:654	the addition	643:654	the addition of AgNPs	643:663	The surface color and light transmittance of the pectin films were greatly influenced by the addition of AgNPs.
27492557	8	56	theme	pectin/AgNPs	1172:1183	arg1	films					1199:1203	The pectin/AgNPs nanocomposite films	1168:1203	The pectin/AgNPs nanocomposite films	1168:1203	The pectin/AgNPs nanocomposite films exhibited strong antibacterial activity against food-borne pathogenic bacteria, Escherichia coli and Listeria monocytogenes.
27492557	6	57	from	decrease	872:879	arg1	transparency					888:899	the transparency	884:899	the transparency	884:899	UV-light barrier property of the pectin film increased significantly with a little decrease in the transparency.
27492557	1	58	theme	pectin-based	301:312	arg1	films					338:342	pectin-based antimicrobial composite films	301:342	pectin-based antimicrobial composite films	301:342	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	1	59	dep	mimosoides	200:209	arg1	CMLE					217:220	CMLE	217:220	CMLE	217:220	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	1	59	dep	mimosoides	200:209	arg1	Lamk					211:214	Lamk	211:214	Caesalpinia mimosoides Lamk (CMLE)	188:221	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	0	60	with	films	53:57	arg1	properties					85:94	properties	85:94	properties	85:94	Preparation of pectin/silver nanoparticles composite films with UV-light barrier and properties.
27492557	0	60	with	films	53:57	arg1	barrier					73:79	UV-light barrier	64:79	UV-light barrier	64:79	Preparation of pectin/silver nanoparticles composite films with UV-light barrier and properties.
27492557	1	61	theme	green	147:151	arg1	method					153:158	a green method	145:158	a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents	145:256	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	7	62	theme	barrier	1118:1124	arg1	properties					1126:1135	barrier properties	1118:1135	barrier properties	1118:1135	Though there were no structural changes in the pectin film by the incorporation of CMLE and AgNPs as indicated by the FTIR results, the film properties such as thermal stability, mechanical strength, and water vapor barrier properties of the pectin films increased.
27492557	1	63	theme	reducing	226:233	arg1	extract					177:183	an aqueous extract	166:183	an aqueous extract of Caesalpinia mimosoides Lamk (CMLE)	166:221	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
27492557	1	63	theme	reducing	226:233	arg1	agents					251:256	reducing and stabilizing agents	226:256	reducing and stabilizing agents	226:256	Silver nanoparticles (AgNPs) was synthesized by a green method using an aqueous extract of Caesalpinia mimosoides Lamk (CMLE) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films.
26102015	11	0	theme	new	2072:2074	arg1	era					2076:2078	a new era	2070:2078	a new era with bivalirudin	2070:2095	Therefore, is it the beginning of a new era with bivalirudin or is it a welcome back to an old friend, heparin?
26102015	7	1	theme	observational	1245:1257	arg1	studies					1259:1265	observational studies	1245:1265	observational studies with bivalirudin	1245:1282	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	7	2	with	studies	1259:1265	arg1	bivalirudin					1272:1282	bivalirudin	1272:1282	bivalirudin	1272:1282	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	10	3	from	reinforcement	1822:1834	arg1	utilization					1848:1858	the sole utilization	1839:1858	the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients	1839:2032	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	7	4	from	studies	1259:1265	arg1	patients					1336:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	7	5	theme	future	1445:1450	arg1	use					1463:1465	the future widespread use	1441:1465	the future widespread use of bivalirudin	1441:1480	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	8	6	theme	composite	1624:1632	arg1	points					1638:1643	the composite end points	1620:1643	the composite end points	1620:1643	"In the setting of PCI and in the absence of GP IIb/IIIa inhibitors, bivalirudin did not offer any beneficial effect in the incidence of the composite end points when compared with heparin.
26102015	11	7	dep	it	2050:2051	arg1	beginning					2057:2065	the beginning	2053:2065	it the beginning of a new era with bivalirudin	2050:2095	Therefore, is it the beginning of a new era with bivalirudin or is it a welcome back to an old friend, heparin?
26102015	10	8	theme	inhibitors	1893:1902	arg1	utilization					1848:1858	the sole utilization	1839:1858	the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients	1839:2032	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	6	9	from	meta-analysis	1081:1093	arg1	Findings					1062:1069	Findings	1062:1069	Findings from this meta-analysis	1062:1093	Findings from this meta-analysis suggest that routine use of bivalirudin offers little advantage over heparin among PCI patients.
26102015	10	10	theme	periprocedural	1965:1978	arg1	complications					1984:1996	periprocedural PCI complications	1965:1996	periprocedural PCI complications	1965:1996	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	10	11	theme	GP	1881:1882	arg1	inhibitors					1893:1902	heparin confining GP IIb/IIIa inhibitors	1863:1902	heparin confining GP IIb/IIIa inhibitors	1863:1902	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	1	12	theme	observational	172:184	arg1	studies					186:192	observational studies	172:192	observational studies	172:192	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	10	13	from	utilization	1848:1858	arg1	reinforcement					1822:1834	reinforcement	1822:1834	reinforcement	1822:1834	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	10	14	theme	antithrombotics	1926:1940	arg1	utilization					1848:1858	the sole utilization	1839:1858	the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients	1839:2032	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	11	15	theme	era	2076:2078	arg1	beginning					2057:2065	the beginning	2053:2065	it the beginning of a new era with bivalirudin	2050:2095	Therefore, is it the beginning of a new era with bivalirudin or is it a welcome back to an old friend, heparin?
26102015	10	16	theme	other	1908:1912	arg1	antithrombotics					1926:1940	other intravenous antithrombotics	1908:1940	other intravenous antithrombotics	1908:1940	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	0	17	theme	Unfractionated	111:124	arg1	Heparin					126:132	an Old Friend Unfractionated Heparin	97:132	an Old Friend Unfractionated Heparin	97:132	Heparin Versus Bivalirudin in Contemporary Percutaneous Coronary Intervention: A Welcome Back to an Old Friend Unfractionated Heparin.
26102015	11	18	with	era	2076:2078	arg1	bivalirudin					2085:2095	bivalirudin	2085:2095	bivalirudin	2085:2095	Therefore, is it the beginning of a new era with bivalirudin or is it a welcome back to an old friend, heparin?
26102015	1	19	theme	strong	201:206	arg1	argument					208:215	a strong argument	199:215	a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI)	199:404	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	1	20	gly	glycoprotein	280:291	arg1	GP					294:295	GP	294:295	GP	294:295	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	1	20	gly	glycoprotein	280:291	arg1	glycoprotein					280:291	systematic glycoprotein	269:291	systematic glycoprotein (GP)	269:296	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	9	21	theme	world	1690:1694	arg1	practice					1696:1703	real world practice	1685:1703	real world practice	1685:1703	For now, in real world practice, one would probably choose a well-known cheaper drug that has already passed the test of time, heparin.
26102015	12	22	theme	unfractionated	2234:2247	arg1	friend					2226:2231	an old friend	2219:2231	an old friend	2219:2231	Indeed, after more than two decades, it is always good to welcome back an old friend, unfractionated heparin.
26102015	12	22	theme	unfractionated	2234:2247	arg1	heparin					2249:2255	unfractionated heparin	2234:2255	unfractionated heparin	2234:2255	Indeed, after more than two decades, it is always good to welcome back an old friend, unfractionated heparin.
26102015	2	23	theme	IIb/IIIa	555:562	arg1	inhibitors					564:573	GP IIb/IIIa inhibitors	552:573	GP IIb/IIIa inhibitors	552:573	However, there is no doubt that the benefit observed with bivalirudin was achieved because of the major bleeding complications with heparin plus GP IIb/IIIa inhibitors.
26102015	10	24	theme	bailout	1945:1951	arg1	therapy					1953:1959	bailout therapy	1945:1959	bailout therapy for periprocedural PCI complications in acute coronary syndrome patients	1945:2032	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	4	25	theme	bleeding	895:902	arg1	complications					904:916	major bleeding complications	889:916	major bleeding complications with bivalirudin	889:933	When this latter drug was compared with unfractionated heparin alone there was no benefit in ischemic complications but a decrease in major bleeding complications with bivalirudin.
26102015	7	26	with	trials	1234:1239	arg1	bivalirudin					1272:1282	bivalirudin	1272:1282	bivalirudin	1272:1282	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	10	27	theme	PCI	1980:1982	arg1	complications					1984:1996	periprocedural PCI complications	1965:1996	periprocedural PCI complications	1965:1996	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	3	28	theme	bleeding	602:609	arg1	complications					611:623	bleeding complications	602:623	bleeding complications	602:623	Therefore, if we diminish bleeding complications by eliminating the systematic utilization of GP IIb/IIIa inhibitors, there would be a lesser benefit with the use of bivalirudin.
26102015	2	29	theme	GP	552:553	arg1	inhibitors					564:573	GP IIb/IIIa inhibitors	552:573	GP IIb/IIIa inhibitors	552:573	However, there is no doubt that the benefit observed with bivalirudin was achieved because of the major bleeding complications with heparin plus GP IIb/IIIa inhibitors.
26102015	7	30	theme	randomized	1223:1232	arg1	trials					1234:1239	some randomized trials	1218:1239	some randomized trials	1218:1239	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	12	31	theme	old	2222:2224	arg1	friend					2226:2231	an old friend	2219:2231	an old friend	2219:2231	Indeed, after more than two decades, it is always good to welcome back an old friend, unfractionated heparin.
26102015	12	31	theme	old	2222:2224	arg1	heparin					2249:2255	unfractionated heparin	2234:2255	unfractionated heparin	2234:2255	Indeed, after more than two decades, it is always good to welcome back an old friend, unfractionated heparin.
26102015	1	32	theme	trials	161:166	arg1	results					139:145	The results	135:145	The results of randomized trials and observational studies	135:192	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	2	33	theme	bleeding	511:518	arg1	complications					520:532	the major bleeding complications	501:532	the major bleeding complications with heparin plus GP IIb/IIIa inhibitors	501:573	However, there is no doubt that the benefit observed with bivalirudin was achieved because of the major bleeding complications with heparin plus GP IIb/IIIa inhibitors.
26102015	0	34	from	Heparin	0:6	arg1	Intervention					65:76	Contemporary Percutaneous Coronary Intervention	30:76	Contemporary Percutaneous Coronary Intervention	30:76	Heparin Versus Bivalirudin in Contemporary Percutaneous Coronary Intervention: A Welcome Back to an Old Friend Unfractionated Heparin.
26102015	10	35	theme	coronary	2007:2014	arg1	syndrome					2016:2023	acute coronary syndrome	2001:2023	acute coronary syndrome patients	2001:2032	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	1	36	theme	patients	344:351	arg1	majority					332:339	the great majority	322:339	the great majority of patients undergoing percutaneous coronary interventions (PCI)	322:404	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	4	37	theme	latter	765:770	arg1	drug					772:775	this latter drug	760:775	this latter drug	760:775	When this latter drug was compared with unfractionated heparin alone there was no benefit in ischemic complications but a decrease in major bleeding complications with bivalirudin.
26102015	0	38	theme	Old	100:102	arg1	Heparin					126:132	an Old Friend Unfractionated Heparin	97:132	an Old Friend Unfractionated Heparin	97:132	Heparin Versus Bivalirudin in Contemporary Percutaneous Coronary Intervention: A Welcome Back to an Old Friend Unfractionated Heparin.
26102015	1	39	theme	percutaneous	364:375	arg1	PCI					401:403	PCI	401:403	PCI	401:403	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	1	39	theme	percutaneous	364:375	arg1	interventions					386:398	percutaneous coronary interventions	364:398	percutaneous coronary interventions (PCI)	364:404	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	5	40	theme	more	978:981	arg1	insights					983:990	more insights	978:990	more insights	978:990	However, a very recent meta-analysis shed more insights on the utilization of bivalirudin versus heparin regimens during PCI.
26102015	10	41	theme	IIb/IIIa	1884:1891	arg1	inhibitors					1893:1902	heparin confining GP IIb/IIIa inhibitors	1863:1902	heparin confining GP IIb/IIIa inhibitors	1863:1902	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	1	42	theme	systematic	269:278	arg1	GP					294:295	GP	294:295	GP	294:295	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	1	42	theme	systematic	269:278	arg1	glycoprotein					280:291	systematic glycoprotein	269:291	systematic glycoprotein (GP)	269:296	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	10	43	from	therapy	1953:1959	arg1	patients					2025:2032	acute coronary syndrome patients	2001:2032	acute coronary syndrome patients	2001:2032	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	8	44	theme	IIb/IIIa	1531:1538	arg1	inhibitors					1540:1549	GP IIb/IIIa inhibitors	1528:1549	GP IIb/IIIa inhibitors	1528:1549	"In the setting of PCI and in the absence of GP IIb/IIIa inhibitors, bivalirudin did not offer any beneficial effect in the incidence of the composite end points when compared with heparin.
26102015	11	45	theme	old	2127:2129	arg1	heparin					2139:2145	heparin	2139:2145	heparin	2139:2145	Therefore, is it the beginning of a new era with bivalirudin or is it a welcome back to an old friend, heparin?
26102015	11	45	theme	old	2127:2129	arg1	friend					2131:2136	an old friend	2124:2136	an old friend	2124:2136	Therefore, is it the beginning of a new era with bivalirudin or is it a welcome back to an old friend, heparin?
26102015	7	46	dep	trials	1234:1239	arg1	done					1345:1348	done	1345:1348	done by myself	1345:1358	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	7	46	dep	trials	1234:1239	arg1	published					1364:1372	published	1364:1372	published almost 4 years ago in this journal	1364:1407	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	7	47	from	reflections	1426:1436	arg1	use					1463:1465	the future widespread use	1441:1465	the future widespread use of bivalirudin	1441:1480	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	0	48	theme	Contemporary	30:41	arg1	Intervention					65:76	Contemporary Percutaneous Coronary Intervention	30:76	Contemporary Percutaneous Coronary Intervention	30:76	Heparin Versus Bivalirudin in Contemporary Percutaneous Coronary Intervention: A Welcome Back to an Old Friend Unfractionated Heparin.
26102015	5	49	theme	bivalirudin	1014:1024	arg1	utilization					999:1009	the utilization	995:1009	the utilization of bivalirudin versus heparin regimens during PCI	995:1059	However, a very recent meta-analysis shed more insights on the utilization of bivalirudin versus heparin regimens during PCI.
26102015	7	50	theme	syndrome	1327:1334	arg1	patients					1336:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	9	51	theme	time	1794:1797	arg1	test					1786:1789	the test	1782:1789	the test of time, heparin	1782:1806	For now, in real world practice, one would probably choose a well-known cheaper drug that has already passed the test of time, heparin.
26102015	0	52	theme	Coronary	56:63	arg1	Intervention					65:76	Contemporary Percutaneous Coronary Intervention	30:76	Contemporary Percutaneous Coronary Intervention	30:76	Heparin Versus Bivalirudin in Contemporary Percutaneous Coronary Intervention: A Welcome Back to an Old Friend Unfractionated Heparin.
26102015	5	53	theme	heparin	1033:1039	arg1	utilization					999:1009	the utilization	995:1009	the utilization of bivalirudin versus heparin regimens during PCI	995:1059	However, a very recent meta-analysis shed more insights on the utilization of bivalirudin versus heparin regimens during PCI.
26102015	7	54	from	patients	1336:1343	arg1	analysis					1206:1213	a detailed analysis	1195:1213	a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal	1195:1407	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	7	55	theme	acute	1312:1316	arg1	patients					1336:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	3	56	theme	inhibitors	682:691	arg1	utilization					655:665	the systematic utilization	640:665	the systematic utilization of GP IIb/IIIa inhibitors	640:691	Therefore, if we diminish bleeding complications by eliminating the systematic utilization of GP IIb/IIIa inhibitors, there would be a lesser benefit with the use of bivalirudin.
26102015	8	57	theme	end	1634:1636	arg1	points					1638:1643	the composite end points	1620:1643	the composite end points	1620:1643	"In the setting of PCI and in the absence of GP IIb/IIIa inhibitors, bivalirudin did not offer any beneficial effect in the incidence of the composite end points when compared with heparin.
26102015	7	58	theme	non-ST-segment	1287:1300	arg1	elevation					1302:1310	non-ST-segment elevation	1287:1310	non-ST-segment elevation acute coronary syndrome patients	1287:1343	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	8	59	theme	beneficial	1582:1591	arg1	effect					1593:1598	any beneficial effect	1578:1598	any beneficial effect	1578:1598	"In the setting of PCI and in the absence of GP IIb/IIIa inhibitors, bivalirudin did not offer any beneficial effect in the incidence of the composite end points when compared with heparin.
26102015	3	60	theme	GP	670:671	arg1	inhibitors					682:691	GP IIb/IIIa inhibitors	670:691	GP IIb/IIIa inhibitors	670:691	Therefore, if we diminish bleeding complications by eliminating the systematic utilization of GP IIb/IIIa inhibitors, there would be a lesser benefit with the use of bivalirudin.
26102015	0	61	dep	Heparin	0:6	arg1	Welcome					81:87	A Welcome	79:87	Heparin Versus Bivalirudin in Contemporary Percutaneous Coronary Intervention: A Welcome Back to an Old Friend Unfractionated Heparin.	0:133	Heparin Versus Bivalirudin in Contemporary Percutaneous Coronary Intervention: A Welcome Back to an Old Friend Unfractionated Heparin.
26102015	6	62	theme	routine	1108:1114	arg1	use					1116:1118	routine use	1108:1118	routine use of bivalirudin	1108:1133	Findings from this meta-analysis suggest that routine use of bivalirudin offers little advantage over heparin among PCI patients.
26102015	10	63	theme	confining	1871:1879	arg1	inhibitors					1893:1902	heparin confining GP IIb/IIIa inhibitors	1863:1902	heparin confining GP IIb/IIIa inhibitors	1863:1902	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	4	64	theme	ischemic	848:855	arg1	complications					857:869	ischemic complications	848:869	ischemic complications	848:869	When this latter drug was compared with unfractionated heparin alone there was no benefit in ischemic complications but a decrease in major bleeding complications with bivalirudin.
26102015	7	65	theme	studies	1259:1265	arg1	analysis					1206:1213	a detailed analysis	1195:1213	a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal	1195:1407	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	10	66	theme	sole	1843:1846	arg1	utilization					1848:1858	the sole utilization	1839:1858	the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients	1839:2032	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	7	67	theme	bivalirudin	1470:1480	arg1	use					1463:1465	the future widespread use	1441:1465	the future widespread use of bivalirudin	1441:1480	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	7	68	theme	widespread	1452:1461	arg1	use					1463:1465	the future widespread use	1441:1465	the future widespread use of bivalirudin	1441:1480	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	7	69	theme	elevation	1302:1310	arg1	patients					1336:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	6	70	theme	PCI	1178:1180	arg1	patients					1182:1189	PCI patients	1178:1189	PCI patients	1178:1189	Findings from this meta-analysis suggest that routine use of bivalirudin offers little advantage over heparin among PCI patients.
26102015	3	71	theme	lesser	711:716	arg1	benefit					718:724	a lesser benefit	709:724	a lesser benefit with the use of bivalirudin	709:752	Therefore, if we diminish bleeding complications by eliminating the systematic utilization of GP IIb/IIIa inhibitors, there would be a lesser benefit with the use of bivalirudin.
26102015	1	72	theme	studies	186:192	arg1	results					139:145	The results	135:145	The results of randomized trials and observational studies	135:192	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	5	73	dep	bivalirudin	1014:1024	arg1	regimens					1041:1048	regimens	1041:1048	regimens	1041:1048	However, a very recent meta-analysis shed more insights on the utilization of bivalirudin versus heparin regimens during PCI.
26102015	3	74	theme	bivalirudin	742:752	arg1	use					735:737	the use	731:737	the use of bivalirudin	731:752	Therefore, if we diminish bleeding complications by eliminating the systematic utilization of GP IIb/IIIa inhibitors, there would be a lesser benefit with the use of bivalirudin.
26102015	9	75	theme	real	1685:1688	arg1	practice					1696:1703	real world practice	1685:1703	real world practice	1685:1703	For now, in real world practice, one would probably choose a well-known cheaper drug that has already passed the test of time, heparin.
26102015	7	76	from	analysis	1206:1213	arg1	patients					1336:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	9	77	theme	well-known	1734:1743	arg1	drug					1753:1756	a well-known cheaper drug	1732:1756	a well-known cheaper drug that has already passed the test of time, heparin	1732:1806	For now, in real world practice, one would probably choose a well-known cheaper drug that has already passed the test of time, heparin.
26102015	10	78	theme	intravenous	1914:1924	arg1	antithrombotics					1926:1940	other intravenous antithrombotics	1908:1940	other intravenous antithrombotics	1908:1940	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	1	79	dep	heparin	256:262	arg1	inhibitors					307:316	IIb/IIIa inhibitors	298:316	IIb/IIIa inhibitors	298:316	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	4	80	theme	major	889:893	arg1	complications					904:916	major bleeding complications	889:916	major bleeding complications with bivalirudin	889:933	When this latter drug was compared with unfractionated heparin alone there was no benefit in ischemic complications but a decrease in major bleeding complications with bivalirudin.
26102015	7	81	from	trials	1234:1239	arg1	patients					1336:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	7	82	theme	trials	1234:1239	arg1	analysis					1206:1213	a detailed analysis	1195:1213	a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal	1195:1407	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	4	83	from	decrease	877:884	arg1	complications					904:916	major bleeding complications	889:916	major bleeding complications with bivalirudin	889:933	When this latter drug was compared with unfractionated heparin alone there was no benefit in ischemic complications but a decrease in major bleeding complications with bivalirudin.
26102015	2	84	with	complications	520:532	arg1	heparin					539:545	heparin	539:545	heparin	539:545	However, there is no doubt that the benefit observed with bivalirudin was achieved because of the major bleeding complications with heparin plus GP IIb/IIIa inhibitors.
26102015	2	84	with	complications	520:532	arg1	inhibitors					564:573	GP IIb/IIIa inhibitors	552:573	GP IIb/IIIa inhibitors	552:573	However, there is no doubt that the benefit observed with bivalirudin was achieved because of the major bleeding complications with heparin plus GP IIb/IIIa inhibitors.
26102015	8	85	theme	PCI	1502:1504	arg1	setting					1491:1497	the setting	1487:1497	the setting of PCI	1487:1504	"In the setting of PCI and in the absence of GP IIb/IIIa inhibitors, bivalirudin did not offer any beneficial effect in the incidence of the composite end points when compared with heparin.
26102015	9	86	theme	cheaper	1745:1751	arg1	drug					1753:1756	a well-known cheaper drug	1732:1756	a well-known cheaper drug that has already passed the test of time, heparin	1732:1806	For now, in real world practice, one would probably choose a well-known cheaper drug that has already passed the test of time, heparin.
26102015	1	87	theme	great	326:330	arg1	majority					332:339	the great majority	322:339	the great majority of patients undergoing percutaneous coronary interventions (PCI)	322:404	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	1	88	theme	randomized	150:159	arg1	trials					161:166	randomized trials	150:166	randomized trials	150:166	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	10	89	theme	syndrome	2016:2023	arg1	patients					2025:2032	acute coronary syndrome patients	2001:2032	acute coronary syndrome patients	2001:2032	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	5	90	theme	recent	952:957	arg1	meta-analysis					959:971	a very recent meta-analysis	945:971	a very recent meta-analysis	945:971	However, a very recent meta-analysis shed more insights on the utilization of bivalirudin versus heparin regimens during PCI.
26102015	2	91	theme	major	505:509	arg1	complications					520:532	the major bleeding complications	501:532	the major bleeding complications with heparin plus GP IIb/IIIa inhibitors	501:573	However, there is no doubt that the benefit observed with bivalirudin was achieved because of the major bleeding complications with heparin plus GP IIb/IIIa inhibitors.
26102015	10	92	theme	acute	2001:2005	arg1	syndrome					2016:2023	acute coronary syndrome	2001:2023	acute coronary syndrome patients	2001:2032	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	0	93	theme	Friend	104:109	arg1	Heparin					126:132	an Old Friend Unfractionated Heparin	97:132	an Old Friend Unfractionated Heparin	97:132	Heparin Versus Bivalirudin in Contemporary Percutaneous Coronary Intervention: A Welcome Back to an Old Friend Unfractionated Heparin.
26102015	4	94	from	benefit	837:843	arg1	complications					857:869	ischemic complications	848:869	ischemic complications	848:869	When this latter drug was compared with unfractionated heparin alone there was no benefit in ischemic complications but a decrease in major bleeding complications with bivalirudin.
26102015	8	95	theme	GP	1528:1529	arg1	inhibitors					1540:1549	GP IIb/IIIa inhibitors	1528:1549	GP IIb/IIIa inhibitors	1528:1549	"In the setting of PCI and in the absence of GP IIb/IIIa inhibitors, bivalirudin did not offer any beneficial effect in the incidence of the composite end points when compared with heparin.
26102015	1	96	theme	coronary	377:384	arg1	PCI					401:403	PCI	401:403	PCI	401:403	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	1	96	theme	coronary	377:384	arg1	interventions					386:398	percutaneous coronary interventions	364:398	percutaneous coronary interventions (PCI)	364:404	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	4	97	with	complications	904:916	arg1	bivalirudin					923:933	bivalirudin	923:933	bivalirudin	923:933	When this latter drug was compared with unfractionated heparin alone there was no benefit in ischemic complications but a decrease in major bleeding complications with bivalirudin.
26102015	8	98	theme	inhibitors	1540:1549	arg1	absence					1517:1523	the absence	1513:1523	the absence of GP IIb/IIIa inhibitors	1513:1549	"In the setting of PCI and in the absence of GP IIb/IIIa inhibitors, bivalirudin did not offer any beneficial effect in the incidence of the composite end points when compared with heparin.
26102015	3	99	theme	systematic	644:653	arg1	utilization					655:665	the systematic utilization	640:665	the systematic utilization of GP IIb/IIIa inhibitors	640:691	Therefore, if we diminish bleeding complications by eliminating the systematic utilization of GP IIb/IIIa inhibitors, there would be a lesser benefit with the use of bivalirudin.
26102015	0	100	theme	Percutaneous	43:54	arg1	Intervention					65:76	Contemporary Percutaneous Coronary Intervention	30:76	Contemporary Percutaneous Coronary Intervention	30:76	Heparin Versus Bivalirudin in Contemporary Percutaneous Coronary Intervention: A Welcome Back to an Old Friend Unfractionated Heparin.
26102015	7	101	theme	coronary	1318:1325	arg1	patients					1336:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	non-ST-segment elevation acute coronary syndrome patients	1287:1343	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	4	102	theme	unfractionated	795:808	arg1	heparin					810:816	unfractionated heparin	795:816	unfractionated heparin alone	795:822	When this latter drug was compared with unfractionated heparin alone there was no benefit in ischemic complications but a decrease in major bleeding complications with bivalirudin.
26102015	7	103	theme	detailed	1197:1204	arg1	analysis					1206:1213	a detailed analysis	1195:1213	a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal	1195:1407	In a detailed analysis of some randomized trials and observational studies with bivalirudin in non-ST-segment elevation acute coronary syndrome patients done by myself and published almost 4 years ago in this journal, I rendered some reflections on the future widespread use of bivalirudin.
26102015	1	104	theme	IIb/IIIa	298:305	arg1	inhibitors					307:316	IIb/IIIa inhibitors	298:316	IIb/IIIa inhibitors	298:316	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
26102015	6	105	theme	little	1142:1147	arg1	advantage					1149:1157	little advantage	1142:1157	little advantage over heparin among PCI patients	1142:1189	Findings from this meta-analysis suggest that routine use of bivalirudin offers little advantage over heparin among PCI patients.
26102015	3	106	theme	IIb/IIIa	673:680	arg1	inhibitors					682:691	GP IIb/IIIa inhibitors	670:691	GP IIb/IIIa inhibitors	670:691	Therefore, if we diminish bleeding complications by eliminating the systematic utilization of GP IIb/IIIa inhibitors, there would be a lesser benefit with the use of bivalirudin.
26102015	8	107	theme	points	1638:1643	arg1	incidence					1607:1615	the incidence	1603:1615	the incidence of the composite end points	1603:1643	"In the setting of PCI and in the absence of GP IIb/IIIa inhibitors, bivalirudin did not offer any beneficial effect in the incidence of the composite end points when compared with heparin.
26102015	6	108	theme	bivalirudin	1123:1133	arg1	use					1116:1118	routine use	1108:1118	routine use of bivalirudin	1108:1133	Findings from this meta-analysis suggest that routine use of bivalirudin offers little advantage over heparin among PCI patients.
26102015	10	109	dep	reinforcement	1822:1834	arg1	"					2034:2034	"	2034:2034	"	2034:2034	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	3	110	with	benefit	718:724	arg1	use					735:737	the use	731:737	the use of bivalirudin	731:752	Therefore, if we diminish bleeding complications by eliminating the systematic utilization of GP IIb/IIIa inhibitors, there would be a lesser benefit with the use of bivalirudin.
26102015	10	111	theme	heparin	1863:1869	arg1	inhibitors					1893:1902	heparin confining GP IIb/IIIa inhibitors	1863:1902	heparin confining GP IIb/IIIa inhibitors	1863:1902	There may be reinforcement in the sole utilization of heparin confining GP IIb/IIIa inhibitors and other intravenous antithrombotics to bailout therapy for periprocedural PCI complications in acute coronary syndrome patients."
26102015	1	112	theme	bivalirudin	232:242	arg1	use					225:227	the use	221:227	the use of bivalirudin	221:242	The results of randomized trials and observational studies make a strong argument for the use of bivalirudin rather than heparin plus systematic glycoprotein (GP) IIb/IIIa inhibitors for the great majority of patients undergoing percutaneous coronary interventions (PCI).
24768890	1	0	from	biomass	187:193	arg1	production					171:180	5-HMF production	165:180	5-HMF production from biomass	165:193	Ionic liquid (IL) has been widely investigated in 5-HMF production from biomass.
24768890	1	1	theme	5-HMF	165:169	arg1	production					171:180	5-HMF production	165:180	5-HMF production from biomass	165:193	Ionic liquid (IL) has been widely investigated in 5-HMF production from biomass.
24768890	7	2	theme	highest	1087:1093	arg1	yield					1101:1105	The highest 5-HMF yield	1083:1105	The highest 5-HMF yield of 28.63%	1083:1115	The highest 5-HMF yield of 28.63% was achieved with the optimal condition.
24768890	3	3	contain	containing	468:477	arg2	LiCl					479:482	LiCl	479:482	LiCl	479:482	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	3	3	contain	containing	468:477	arg1	N-dimethylacetamide					441:459	N-dimethylacetamide	441:459	N-dimethylacetamide (DMAc) containing LiCl	441:482	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	3	3	contain	containing	468:477	arg1	N					439:439	N	439:439	N	439:439	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	3	3	contain	containing	468:477	arg1	DMAc					462:465	DMAc	462:465	DMAc	462:465	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	4	4	from	gas	719:721	arg1	absorption					683:692	the absorption	679:692	the absorption of SO2 and CO2 from flue gas	679:721	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	4	5	theme	first	795:799	arg1	time					801:804	the first time	791:804	the first time	791:804	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	5	6	from	activity	865:872	arg1	MCC					899:901	MCC	899:901	MCC	899:901	Of the catalysts employed, [TMG]BF4 showed high catalytic activity in 5-HMF production from MCC.
24768890	5	6	from	activity	865:872	arg1	production					883:892	5-HMF production	877:892	5-HMF production from MCC	877:901	Of the catalysts employed, [TMG]BF4 showed high catalytic activity in 5-HMF production from MCC.
24768890	6	7	theme	MCC	977:979	arg1	conversion					981:990	MCC conversion	977:990	MCC conversion	977:990	The condition including the ratio of IL to MCC, temperature and time for MCC conversion was optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).
24768890	2	8	theme	IL	286:287	arg1	IL					286:287	IL	286:287	IL	286:287	However, most of studies employed IL as reaction solvent which requires a large amount of IL.
24768890	2	8	theme	IL	286:287	arg1	amount					276:281	a large amount	268:281	a large amount of IL	268:287	However, most of studies employed IL as reaction solvent which requires a large amount of IL.
24768890	5	9	theme	catalytic	855:863	arg1	activity					865:872	high catalytic activity	850:872	high catalytic activity in 5-HMF production from MCC	850:901	Of the catalysts employed, [TMG]BF4 showed high catalytic activity in 5-HMF production from MCC.
24768890	4	10	theme	CO2	705:707	arg1	absorption					683:692	the absorption	679:692	the absorption of SO2 and CO2 from flue gas	679:721	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	0	11	theme	Microwave-assisted	0:17	arg1	conversion					19:28	Microwave-assisted conversion	0:28	Microwave-assisted conversion of microcrystalline cellulose to 5-hydroxymethylfurfural	0:85	Microwave-assisted conversion of microcrystalline cellulose to 5-hydroxymethylfurfural catalyzed by ionic liquids.
24768890	4	12	theme	1,1,3,3-tetramethylguanidine	541:568	arg1	[TMG					589:592	[TMG	589:592	[TMG	589:592	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	4	12	theme	1,1,3,3-tetramethylguanidine	541:568	arg1	tetrafluoroborate					570:586	1,1,3,3-tetramethylguanidine tetrafluoroborate	541:586	1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4])	541:599	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	4	13	theme	MCC	774:776	arg1	conversion					760:769	the conversion	756:769	the conversion of MCC to 5-HMF for the first time	756:804	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	4	14	used	used	671:674	arg2	[TMG					589:592	[TMG	589:592	[TMG	589:592	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	4	14	used	used	671:674	arg2	tetrafluoroborate					570:586	1,1,3,3-tetramethylguanidine tetrafluoroborate	541:586	1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4])	541:599	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	4	14	used	used	671:674	arg2	lactate					634:640	1,1,3,3-tetramethylguanidine lactate	605:640	1,1,3,3-tetramethylguanidine lactate ([TMG]L)	605:649	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	5	15	theme	5-HMF	877:881	arg1	production					883:892	5-HMF production	877:892	5-HMF production from MCC	877:901	Of the catalysts employed, [TMG]BF4 showed high catalytic activity in 5-HMF production from MCC.
24768890	3	16	used	utilized	319:326	arg2	catalyst					331:338	catalyst	331:338	catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF	331:401	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	3	16	used	utilized	319:326	arg2	IL					312:313	IL	312:313	IL	312:313	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	2	17	theme	large	270:274	arg1	IL					286:287	IL	286:287	IL	286:287	However, most of studies employed IL as reaction solvent which requires a large amount of IL.
24768890	2	17	theme	large	270:274	arg1	amount					276:281	a large amount	268:281	a large amount of IL	268:287	However, most of studies employed IL as reaction solvent which requires a large amount of IL.
24768890	3	18	theme	microwave	409:417	arg1	MI					432:433	MI	432:433	MI	432:433	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	3	18	theme	microwave	409:417	arg1	irradiation					419:429	microwave irradiation	409:429	microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl	409:482	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	7	19	theme	5-HMF	1095:1099	arg1	yield					1101:1105	The highest 5-HMF yield	1083:1105	The highest 5-HMF yield of 28.63%	1083:1115	The highest 5-HMF yield of 28.63% was achieved with the optimal condition.
24768890	5	20	from	MCC	899:901	arg1	activity					865:872	high catalytic activity	850:872	high catalytic activity in 5-HMF production from MCC	850:901	Of the catalysts employed, [TMG]BF4 showed high catalytic activity in 5-HMF production from MCC.
24768890	5	20	from	MCC	899:901	arg1	production					883:892	5-HMF production	877:892	5-HMF production from MCC	877:901	Of the catalysts employed, [TMG]BF4 showed high catalytic activity in 5-HMF production from MCC.
24768890	3	21	theme	microcrystalline	361:376	arg1	MCC					389:391	MCC	389:391	MCC	389:391	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	3	21	theme	microcrystalline	361:376	arg1	cellulose					378:386	microcrystalline cellulose	361:386	microcrystalline cellulose (MCC)	361:392	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	0	22	theme	microcrystalline	33:48	arg1	cellulose					50:58	microcrystalline cellulose	33:58	microcrystalline cellulose	33:58	Microwave-assisted conversion of microcrystalline cellulose to 5-hydroxymethylfurfural catalyzed by ionic liquids.
24768890	5	23	theme	high	850:853	arg1	activity					865:872	high catalytic activity	850:872	high catalytic activity in 5-HMF production from MCC	850:901	Of the catalysts employed, [TMG]BF4 showed high catalytic activity in 5-HMF production from MCC.
24768890	6	24	theme	Response	1047:1054	arg1	RSM					1077:1079	RSM	1077:1079	RSM	1077:1079	The condition including the ratio of IL to MCC, temperature and time for MCC conversion was optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).
24768890	6	24	theme	Response	1047:1054	arg1	Methodology					1064:1074	Response Surface Methodology	1047:1074	Response Surface Methodology (RSM)	1047:1080	The condition including the ratio of IL to MCC, temperature and time for MCC conversion was optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).
24768890	4	25	theme	SO2	697:699	arg1	absorption					683:692	the absorption	679:692	the absorption of SO2 and CO2 from flue gas	679:721	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	4	26	theme	-based	519:524	arg1	tetrafluoroborate					570:586	1,1,3,3-tetramethylguanidine tetrafluoroborate	541:586	1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4])	541:599	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	4	26	theme	-based	519:524	arg1	lactate					634:640	1,1,3,3-tetramethylguanidine lactate	605:640	1,1,3,3-tetramethylguanidine lactate ([TMG]L)	605:649	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	4	26	theme	-based	519:524	arg1	ILs					526:528	1,1,3,3-tetramethylguanidine (TMG)-based ILs	485:528	1,1,3,3-tetramethylguanidine (TMG)-based ILs	485:528	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	6	27	theme	IL	941:942	arg1	ratio					932:936	the ratio	928:936	the ratio of IL to MCC, temperature and time for MCC conversion	928:990	The condition including the ratio of IL to MCC, temperature and time for MCC conversion was optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).
24768890	2	28	theme	reaction	236:243	arg1	solvent					245:251	reaction solvent	236:251	reaction solvent which requires a large amount of IL	236:287	However, most of studies employed IL as reaction solvent which requires a large amount of IL.
24768890	4	29	theme	1,1,3,3-tetramethylguanidine	605:632	arg1	lactate					634:640	1,1,3,3-tetramethylguanidine lactate	605:640	1,1,3,3-tetramethylguanidine lactate ([TMG]L)	605:649	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	0	30	theme	cellulose	50:58	arg1	conversion					19:28	Microwave-assisted conversion	0:28	Microwave-assisted conversion of microcrystalline cellulose to 5-hydroxymethylfurfural	0:85	Microwave-assisted conversion of microcrystalline cellulose to 5-hydroxymethylfurfural catalyzed by ionic liquids.
24768890	3	31	from	catalyst	331:338	arg1	conversion					347:356	the conversion	343:356	the conversion of microcrystalline cellulose (MCC) to 5-HMF	343:401	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	3	32	from	irradiation	419:429	arg1	N-dimethylacetamide					441:459	N-dimethylacetamide	441:459	N-dimethylacetamide (DMAc) containing LiCl	441:482	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	3	32	from	irradiation	419:429	arg1	N					439:439	N	439:439	N	439:439	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	3	33	theme	cellulose	378:386	arg1	conversion					347:356	the conversion	343:356	the conversion of microcrystalline cellulose (MCC) to 5-HMF	343:401	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	6	34	theme	Composite	1020:1028	arg1	CCD					1038:1040	CCD	1038:1040	CCD	1038:1040	The condition including the ratio of IL to MCC, temperature and time for MCC conversion was optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).
24768890	6	34	theme	Composite	1020:1028	arg1	Design					1030:1035	Central Composite Design	1012:1035	Central Composite Design (CCD)	1012:1041	The condition including the ratio of IL to MCC, temperature and time for MCC conversion was optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).
24768890	6	35	theme	Surface	1056:1062	arg1	RSM					1077:1079	RSM	1077:1079	RSM	1077:1079	The condition including the ratio of IL to MCC, temperature and time for MCC conversion was optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).
24768890	6	35	theme	Surface	1056:1062	arg1	Methodology					1064:1074	Response Surface Methodology	1047:1074	Response Surface Methodology (RSM)	1047:1080	The condition including the ratio of IL to MCC, temperature and time for MCC conversion was optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).
24768890	3	36	theme	present	297:303	arg1	study					305:309	the present study	293:309	the present study	293:309	In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl.
24768890	6	37	theme	Central	1012:1018	arg1	CCD					1038:1040	CCD	1038:1040	CCD	1038:1040	The condition including the ratio of IL to MCC, temperature and time for MCC conversion was optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).
24768890	6	37	theme	Central	1012:1018	arg1	Design					1030:1035	Central Composite Design	1012:1035	Central Composite Design (CCD)	1012:1041	The condition including the ratio of IL to MCC, temperature and time for MCC conversion was optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM).
24768890	5	38	theme	[TMG	834:837	arg1	BF4					839:841	[TMG]BF4	834:841	[TMG]BF4	834:841	Of the catalysts employed, [TMG]BF4 showed high catalytic activity in 5-HMF production from MCC.
24768890	4	39	theme	flue	714:717	arg1	gas					719:721	flue gas	714:721	flue gas	714:721	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	4	40	dep	[TMG	589:592	arg1	[BF4					594:597	[BF4	594:597	[BF4	594:597	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	4	41	dep	[TMG	643:646	arg1	L					648:648	L	648:648	L	648:648	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	0	42	theme	ionic	100:104	arg1	liquids					106:112	ionic liquids	100:112	ionic liquids	100:112	Microwave-assisted conversion of microcrystalline cellulose to 5-hydroxymethylfurfural catalyzed by ionic liquids.
24768890	7	43	theme	%	1115:1115	arg1	yield					1101:1105	The highest 5-HMF yield	1083:1105	The highest 5-HMF yield of 28.63%	1083:1115	The highest 5-HMF yield of 28.63% was achieved with the optimal condition.
24768890	4	44	dep	lactate	634:640	arg1	[TMG					643:646	[TMG	643:646	[TMG	643:646	1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time.
24768890	7	45	theme	optimal	1139:1145	arg1	condition					1147:1155	the optimal condition	1135:1155	the optimal condition	1135:1155	The highest 5-HMF yield of 28.63% was achieved with the optimal condition.
28498117	0	0	theme	carbon	91:96	arg1	fabrication					60:70	the fabrication	56:70	the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2	56:142	Application of response surface methodology to optimize the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2.
28498117	1	1	theme	ion	321:323	arg1	adsorption					302:311	adsorption	302:311	adsorption of Cu2+ ion	302:323	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	1	2	theme	present	149:155	arg1	study					157:161	The present study	145:161	The present study	145:161	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	0	3	theme	ZnCl2-activated	75:89	arg1	carbon					91:96	ZnCl2-activated carbon	75:96	ZnCl2-activated carbon from sugarcane bagasse	75:119	Application of response surface methodology to optimize the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2.
28498117	4	4	theme	correlation	852:862	arg1	coefficients					864:875	high correlation coefficients	847:875	high correlation coefficients	847:875	The statistically significant, well-fitting quadratic regression models were successfully developed as confirmed by high F- and low P-values (<0.0001), high correlation coefficients and lack-of-fit tests.
28498117	1	5	theme	activated	253:261	arg1	AC					271:272	AC	271:272	AC	271:272	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	1	5	theme	activated	253:261	arg1	carbon					263:268	activated carbon	253:268	activated carbon (AC) from sugarcane bagasse	253:296	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	0	6	theme	sugarcane	103:111	arg1	bagasse					113:119	sugarcane bagasse	103:119	sugarcane bagasse	103:119	Application of response surface methodology to optimize the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2.
28498117	3	7	theme	aqueous	674:680	arg1	environment					682:692	the aqueous environment	670:692	the aqueous environment	670:692	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	1	8	from	bagasse	290:296	arg1	AC					271:272	AC	271:272	AC	271:272	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	1	8	from	bagasse	290:296	arg1	fabrication					238:248	the fabrication	234:248	the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion	234:323	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	1	8	from	bagasse	290:296	arg1	carbon					263:268	activated carbon	253:268	activated carbon (AC) from sugarcane bagasse	253:296	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	6	9	theme	large	1187:1191	arg1	volume					1198:1203	large pore volume	1187:1203	large pore volume	1187:1203	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	3	10	theme	parameters	534:543	arg1	effect					512:517	the individual and interactive effect	481:517	the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment	481:692	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	4	11	theme	regression	749:758	arg1	models					760:765	The statistically significant, well-fitting quadratic regression models	695:765	models	760:765	The statistically significant, well-fitting quadratic regression models were successfully developed as confirmed by high F- and low P-values (<0.0001), high correlation coefficients and lack-of-fit tests.
28498117	1	12	theme	carbon	263:268	arg1	fabrication					238:248	the fabrication	234:248	the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion	234:323	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	6	13	theme	m2/g	1167:1170	arg1	area					1146:1149	a surprisingly high surface area	1118:1149	a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature	1118:1260	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	3	14	theme	individual	485:494	arg1	effect					512:517	the individual and interactive effect	481:517	the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment	481:692	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	6	15	theme	optimal	1085:1091	arg1	parameters					1093:1102	the predicted optimal parameters	1071:1102	the predicted optimal parameters	1071:1102	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	1	16	theme	response	193:200	arg1	methodology					210:220	response surface methodology	193:220	response surface methodology	193:220	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	6	17	theme	surface	1138:1144	arg1	area					1146:1149	a surprisingly high surface area	1118:1149	a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature	1118:1260	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	3	18	theme	central	413:419	arg1	design					431:436	The central composite design	409:436	The central composite design	409:436	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	6	19	theme	micropore	1216:1224	arg1	size					1226:1229	narrow micropore size	1209:1229	narrow micropore size	1209:1229	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	3	20	theme	influential	522:532	arg1	parameters					534:543	influential parameters	522:543	influential parameters	522:543	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	3	20	theme	influential	522:532	arg1	ratio					599:603	ZnCl2 impregnation ratio	580:603	ZnCl2 impregnation ratio	580:603	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	3	20	theme	influential	522:532	arg1	temperature					567:577	activation temperature	556:577	activation temperature	556:577	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	3	20	theme	influential	522:532	arg1	time					620:623	activation time	609:623	activation time	609:623	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	3	21	theme	composite	421:429	arg1	design					431:436	The central composite design	409:436	The central composite design	409:436	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	4	22	theme	high	847:850	arg1	coefficients					864:875	high correlation coefficients	847:875	high correlation coefficients	847:875	The statistically significant, well-fitting quadratic regression models were successfully developed as confirmed by high F- and low P-values (<0.0001), high correlation coefficients and lack-of-fit tests.
28498117	4	23	theme	quadratic	739:747	arg1	models					760:765	The statistically significant, well-fitting quadratic regression models	695:765	models	760:765	The statistically significant, well-fitting quadratic regression models were successfully developed as confirmed by high F- and low P-values (<0.0001), high correlation coefficients and lack-of-fit tests.
28498117	4	24	theme	well-fitting	726:737	arg1	models					760:765	The statistically significant, well-fitting quadratic regression models	695:765	models	760:765	The statistically significant, well-fitting quadratic regression models were successfully developed as confirmed by high F- and low P-values (<0.0001), high correlation coefficients and lack-of-fit tests.
28498117	2	25	theme	chemical	353:360	arg1	activation					362:371	chemical activation	353:371	chemical activation with ZnCl2	353:382	The AC was synthesized via chemical activation with ZnCl2 as the activating agent.
28498117	0	26	theme	surface	24:30	arg1	methodology					32:42	response surface methodology	15:42	response surface methodology	15:42	Application of response surface methodology to optimize the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2.
28498117	3	27	from	effect	512:517	arg1	removal					645:651	removal	645:651	removal of Cu2+ ion from the aqueous environment	645:692	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	3	27	from	effect	512:517	arg1	yield					635:639	the AC yield	628:639	the AC yield	628:639	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	5	28	theme	Cu2+	960:963	arg1	efficiency					946:955	removal efficiency	938:955	removal efficiency	938:955	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	28	theme	Cu2+	960:963	arg1	%					1012:1012	92.7%	1008:1012	92.7%	1008:1012	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	28	theme	Cu2+	960:963	arg1	%					1002:1002	48.8%	998:1002	48.8%	998:1002	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	28	theme	Cu2+	960:963	arg1	yield					928:932	the optimum AC yield	913:932	the optimum AC yield	913:932	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	0	29	theme	response	15:22	arg1	methodology					32:42	response surface methodology	15:42	response surface methodology	15:42	Application of response surface methodology to optimize the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2.
28498117	5	30	theme	removal	938:944	arg1	efficiency					946:955	removal efficiency	938:955	removal efficiency	938:955	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	30	theme	removal	938:944	arg1	yield					928:932	the optimum AC yield	913:932	the optimum AC yield	913:932	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	30	theme	removal	938:944	arg1	%					1002:1002	48.8%	998:1002	48.8%	998:1002	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	30	theme	removal	938:944	arg1	%					1012:1012	92.7%	1008:1012	92.7%	1008:1012	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	3	31	theme	activation	556:565	arg1	temperature					567:577	activation temperature	556:577	activation temperature	556:577	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	2	32	theme	activating	391:400	arg1	AC					330:331	The AC	326:331	The AC	326:331	The AC was synthesized via chemical activation with ZnCl2 as the activating agent.
28498117	2	32	theme	activating	391:400	arg1	agent					402:406	the activating agent	387:406	the activating agent	387:406	The AC was synthesized via chemical activation with ZnCl2 as the activating agent.
28498117	6	33	theme	predicted	1075:1083	arg1	parameters					1093:1102	the predicted optimal parameters	1071:1102	the predicted optimal parameters	1071:1102	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	1	34	theme	sugarcane	280:288	arg1	bagasse					290:296	sugarcane bagasse	280:296	sugarcane bagasse	280:296	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	0	35	theme	Cu2	140:142	arg1	removal					129:135	the removal	125:135	the removal of Cu2	125:142	Application of response surface methodology to optimize the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2.
28498117	5	36	theme	actual	1044:1049	arg1	values					1051:1056	the actual values	1040:1056	the actual values	1040:1056	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	4	37	theme	low	823:825	arg1	P-values					827:834	low P-values	823:834	low P-values	823:834	The statistically significant, well-fitting quadratic regression models were successfully developed as confirmed by high F- and low P-values (<0.0001), high correlation coefficients and lack-of-fit tests.
28498117	1	38	theme	surface	202:208	arg1	methodology					210:220	response surface methodology	193:220	response surface methodology	193:220	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	0	39	theme	methodology	32:42	arg1	Application					0:10	Application	0:10	Application of response surface methodology	0:42	Application of response surface methodology to optimize the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2.
28498117	3	40	theme	AC	632:633	arg1	yield					635:639	the AC yield	628:639	the AC yield	628:639	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	5	41	theme	optimum	917:923	arg1	efficiency					946:955	removal efficiency	938:955	removal efficiency	938:955	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	41	theme	optimum	917:923	arg1	%					1012:1012	92.7%	1008:1012	92.7%	1008:1012	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	41	theme	optimum	917:923	arg1	%					1002:1002	48.8%	998:1002	48.8%	998:1002	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	41	theme	optimum	917:923	arg1	yield					928:932	the optimum AC yield	913:932	the optimum AC yield	913:932	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	1	42	theme	methodology	210:220	arg1	application					178:188	the application	174:188	the application of response surface methodology	174:220	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	3	43	from	environment	682:692	arg1	removal					645:651	removal	645:651	removal of Cu2+ ion from the aqueous environment	645:692	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	3	43	from	environment	682:692	arg1	yield					635:639	the AC yield	628:639	the AC yield	628:639	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	0	44	from	fabrication	60:70	arg1	bagasse					113:119	sugarcane bagasse	103:119	sugarcane bagasse	103:119	Application of response surface methodology to optimize the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2.
28498117	3	45	theme	ZnCl2	580:584	arg1	ratio					599:603	ZnCl2 impregnation ratio	580:603	ZnCl2 impregnation ratio	580:603	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	3	46	theme	based	438:442	arg1	experiments					444:454	The central composite design based experiments	409:454	The central composite design based experiments	409:454	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	1	47	from	fabrication	238:248	arg1	bagasse					290:296	sugarcane bagasse	280:296	sugarcane bagasse	280:296	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	3	48	theme	ion	661:663	arg1	removal					645:651	removal	645:651	removal of Cu2+ ion from the aqueous environment	645:692	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	3	48	theme	ion	661:663	arg1	yield					635:639	the AC yield	628:639	the AC yield	628:639	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	3	49	theme	impregnation	586:597	arg1	ratio					599:603	ZnCl2 impregnation ratio	580:603	ZnCl2 impregnation ratio	580:603	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	4	50	theme	lack-of-fit	881:891	arg1	tests					893:897	lack-of-fit tests	881:897	lack-of-fit tests	881:897	The statistically significant, well-fitting quadratic regression models were successfully developed as confirmed by high F- and low P-values (<0.0001), high correlation coefficients and lack-of-fit tests.
28498117	6	51	theme	fabrication	1238:1248	arg1	temperature					1250:1260	low fabrication temperature	1234:1260	low fabrication temperature	1234:1260	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	0	52	from	bagasse	113:119	arg1	fabrication					60:70	the fabrication	56:70	the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2	56:142	Application of response surface methodology to optimize the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2.
28498117	0	52	from	bagasse	113:119	arg1	carbon					91:96	ZnCl2-activated carbon	75:96	ZnCl2-activated carbon from sugarcane bagasse	75:119	Application of response surface methodology to optimize the fabrication of ZnCl2-activated carbon from sugarcane bagasse for the removal of Cu2.
28498117	6	53	theme	narrow	1209:1214	arg1	size					1226:1229	narrow micropore size	1209:1229	narrow micropore size	1209:1229	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	6	54	theme	pore	1193:1196	arg1	volume					1198:1203	large pore volume	1187:1203	large pore volume	1187:1203	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	5	55	theme	AC	925:926	arg1	efficiency					946:955	removal efficiency	938:955	removal efficiency	938:955	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	55	theme	AC	925:926	arg1	%					1012:1012	92.7%	1008:1012	92.7%	1008:1012	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	55	theme	AC	925:926	arg1	%					1002:1002	48.8%	998:1002	48.8%	998:1002	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	5	55	theme	AC	925:926	arg1	yield					928:932	the optimum AC yield	913:932	the optimum AC yield	913:932	Accordingly, the optimum AC yield and removal efficiency of Cu2+ were predicted, respectively, as 48.8% and 92.7% which were approximate to the actual values.
28498117	6	56	theme	high	1133:1136	arg1	area					1146:1149	a surprisingly high surface area	1118:1149	a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature	1118:1260	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	3	57	dep	based	438:442	arg1	design					431:436	The central composite design	409:436	The central composite design	409:436	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	4	58	theme	significant	713:723	arg1	models					760:765	The statistically significant, well-fitting quadratic regression models	695:765	models	760:765	The statistically significant, well-fitting quadratic regression models were successfully developed as confirmed by high F- and low P-values (<0.0001), high correlation coefficients and lack-of-fit tests.
28498117	3	59	theme	Cu2+	656:659	arg1	ion					661:663	Cu2+ ion	656:663	Cu2+ ion	656:663	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	3	60	theme	activation	609:618	arg1	time					620:623	activation time	609:623	activation time	609:623	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	3	61	theme	interactive	500:510	arg1	effect					512:517	the individual and interactive effect	481:517	the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment	481:692	The central composite design based experiments were performed to assess the individual and interactive effect of influential parameters, including activation temperature, ZnCl2 impregnation ratio and activation time on the AC yield and removal of Cu2+ ion from the aqueous environment.
28498117	6	62	from	temperature	1250:1260	arg1	size					1226:1229	narrow micropore size	1209:1229	narrow micropore size	1209:1229	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	6	62	from	temperature	1250:1260	arg1	volume					1198:1203	large pore volume	1187:1203	large pore volume	1187:1203	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
28498117	2	63	with	activation	362:371	arg1	ZnCl2					378:382	ZnCl2	378:382	ZnCl2	378:382	The AC was synthesized via chemical activation with ZnCl2 as the activating agent.
28498117	1	64	theme	Cu2+	316:319	arg1	ion					321:323	Cu2+ ion	316:323	Cu2+ ion	316:323	The present study focused on the application of response surface methodology to optimize the fabrication of activated carbon (AC) from sugarcane bagasse for adsorption of Cu2+ ion.
28498117	4	65	theme	high	811:814	arg1	<0.0001					837:843	<0.0001	837:843	<0.0001	837:843	The statistically significant, well-fitting quadratic regression models were successfully developed as confirmed by high F- and low P-values (<0.0001), high correlation coefficients and lack-of-fit tests.
28498117	4	65	theme	high	811:814	arg1	F-					816:817	high F-	811:817	high F-	811:817	The statistically significant, well-fitting quadratic regression models were successfully developed as confirmed by high F- and low P-values (<0.0001), high correlation coefficients and lack-of-fit tests.
28498117	6	66	theme	low	1234:1236	arg1	temperature					1250:1260	low fabrication temperature	1234:1260	low fabrication temperature	1234:1260	By applying the predicted optimal parameters, the AC shows a surprisingly high surface area of around 1,500 m2/g accompanied by large pore volume and narrow micropore size at low fabrication temperature.
25153326	0	0	from	biopatterning	18:30	arg1	substrates					50:59	ultra-flexible substrates	35:59	ultra-flexible substrates	35:59	Metallization and biopatterning on ultra-flexible substrates via dextran sacrificial layers.
25153326	5	1	theme	PDMS	1156:1159	arg1	wetting					1106:1112	wetting	1106:1112	wetting	1106:1112	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	2	2	dep	layers	530:535	arg1	moduli					546:551	elastic moduli	538:551	elastic moduli down to 3 kPa	538:565	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	5	3	theme	mechanical	1293:1302	arg1	response					1304:1311	cell mechanical response	1288:1311	cell mechanical response	1288:1311	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	2	4	theme	PDMS	524:527	arg1	layers					530:535	ultra-flexible polydimethylsiloxane (PDMS) layers	487:535	ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films	487:621	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	1	5	theme	pattern	186:192	arg1	features					194:201	pattern features	186:201	pattern features onto rigid silicon and glass substrates	186:241	Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors.
25153326	5	6	theme	response	1304:1311	arg1	study					1279:1283	study	1279:1283	study of cell mechanical response	1279:1311	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	4	7	from	etch	979:982	arg1	water					1002:1006	water	1002:1006	water	1002:1006	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	4	8	theme	sacrificial	967:977	arg1	etch					979:982	sacrificial etch	967:982	sacrificial etch of the dextran in water	967:1006	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	0	9	from	Metallization	0:12	arg1	substrates					50:59	ultra-flexible substrates	35:59	ultra-flexible substrates	35:59	Metallization and biopatterning on ultra-flexible substrates via dextran sacrificial layers.
25153326	5	10	theme	epoxy	1256:1260	arg1	structures					1242:1251	composite structures	1232:1251	composite structures of epoxy or particles for study of cell mechanical response	1232:1311	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	2	11	theme	down	553:556	arg1	moduli					546:551	elastic moduli	538:551	elastic moduli down to 3 kPa	538:565	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	5	12	theme	adsorption	1126:1135	arg1	characteristics					1137:1151	surface wetting and protein adsorption characteristics	1098:1151	characteristics	1137:1151	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	4	13	theme	crosslinker	897:907	arg1	ratios					909:914	crosslinker ratios	897:914	crosslinker ratios	897:914	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	5	14	theme	vinyl	1362:1366	arg1	adhesives					1378:1386	vinyl molecular adhesives	1362:1386	vinyl molecular adhesives	1362:1386	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	2	15	theme	simple	405:410	arg1	method					412:417	a simple method	403:417	a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films	403:621	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	2	16	theme	elastic	538:544	arg1	moduli					546:551	elastic moduli	538:551	elastic moduli down to 3 kPa	538:565	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	1	17	theme	Micro-patterning	93:108	arg1	tools					110:114	Micro-patterning tools	93:114	Micro-patterning tools adopted from the semiconductor industry	93:154	Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors.
25153326	5	18	theme	adhesives	1378:1386	arg1	use					1355:1357	use	1355:1357	use of vinyl molecular adhesives	1355:1386	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	2	19	theme	structures	471:480	arg1	structures					471:480	structures	471:480	structures	471:480	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	2	19	theme	structures	471:480	arg1	variety					436:442	a variety	434:442	a variety of patterned materials and structures	434:480	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	2	19	theme	structures	471:480	arg1	materials					457:465	patterned materials	447:465	patterned materials	447:465	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	5	20	theme	composite	1232:1240	arg1	structures					1242:1251	composite structures	1232:1251	composite structures of epoxy or particles for study of cell mechanical response	1232:1311	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	6	21	theme	complete	1424:1431	arg1	range					1440:1444	the complete moduli range	1420:1444	the complete moduli range of PDMS	1420:1452	This method is compatible over the complete moduli range of PDMS, and potentially generalizable over a host of additional micro- and nano-structures and materials.
25153326	6	22	theme	moduli	1433:1438	arg1	range					1440:1444	the complete moduli range	1420:1444	the complete moduli range of PDMS	1420:1452	This method is compatible over the complete moduli range of PDMS, and potentially generalizable over a host of additional micro- and nano-structures and materials.
25153326	1	23	theme	rigid	208:212	arg1	silicon					214:220	rigid silicon	208:220	rigid silicon	208:220	Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors.
25153326	1	24	theme	soft	285:288	arg1	substrates					290:299	soft substrates	285:299	soft substrates	285:299	Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors.
25153326	4	25	dep	materials	754:762	arg1	including					780:788	including	780:788	including dextran itself	780:803	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	2	26	theme	materials	457:465	arg1	structures					471:480	structures	471:480	structures	471:480	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	2	26	theme	materials	457:465	arg1	variety					436:442	a variety	434:442	a variety of patterned materials and structures	434:480	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	2	26	theme	materials	457:465	arg1	materials					457:465	patterned materials	447:465	patterned materials	447:465	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	4	27	theme	dextran	943:949	arg1	layer					951:955	the patterned dextran layer	929:955	the patterned dextran layer	929:955	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	4	28	dep	PDMS	872:875	arg1	base					889:892	10 to 70∶1 base	878:892	10 to 70∶1 base to crosslinker ratios	878:914	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	2	29	theme	patterned	447:455	arg1	materials					457:465	patterned materials	447:465	patterned materials	447:465	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	4	30	theme	patterned	933:941	arg1	layer					951:955	the patterned dextran layer	929:955	the patterned dextran layer	929:955	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	6	31	theme	PDMS	1449:1452	arg1	range					1440:1444	the complete moduli range	1420:1444	the complete moduli range of PDMS	1420:1452	This method is compatible over the complete moduli range of PDMS, and potentially generalizable over a host of additional micro- and nano-structures and materials.
25153326	2	32	theme	ultra-flexible	487:500	arg1	layers					530:535	ultra-flexible polydimethylsiloxane (PDMS) layers	487:535	ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films	487:621	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	1	33	theme	glass	226:230	arg1	substrates					232:241	glass substrates	226:241	glass substrates	226:241	Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors.
25153326	5	34	theme	molecular	1368:1376	arg1	adhesives					1378:1386	vinyl molecular adhesives	1362:1386	vinyl molecular adhesives	1362:1386	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	1	35	from	need	266:269	arg1	substrates					290:299	soft substrates	285:299	soft substrates	285:299	Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors.
25153326	3	36	theme	metal	687:691	arg1	deposition					693:702	metal deposition	687:702	metal deposition	687:702	Dextran films provided a stable template for photolithography, metal deposition, particle adsorption, and protein stamping.
25153326	3	37	theme	Dextran	624:630	arg1	films					632:636	Dextran films	624:636	Dextran films	624:636	Dextran films provided a stable template for photolithography, metal deposition, particle adsorption, and protein stamping.
25153326	6	38	theme	micro-	1511:1516	arg1	host					1492:1495	a host	1490:1495	a host of additional micro- and nano-structures and materials	1490:1550	This method is compatible over the complete moduli range of PDMS, and potentially generalizable over a host of additional micro- and nano-structures and materials.
25153326	2	39	theme	polydimethylsiloxane	502:521	arg1	layers					530:535	ultra-flexible polydimethylsiloxane (PDMS) layers	487:535	ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films	487:621	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	5	40	theme	particles	1265:1273	arg1	structures					1242:1251	composite structures	1232:1251	composite structures of epoxy or particles for study of cell mechanical response	1232:1311	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	0	41	theme	ultra-flexible	35:48	arg1	substrates					50:59	ultra-flexible substrates	35:59	ultra-flexible substrates	35:59	Metallization and biopatterning on ultra-flexible substrates via dextran sacrificial layers.
25153326	5	42	with	metals	1343:1348	arg1	use					1355:1357	use	1355:1357	use of vinyl molecular adhesives	1355:1386	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	4	43	theme	elastomer	844:852	arg1	surface					854:860	an elastomer surface	841:860	an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water	841:1006	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	5	44	theme	cell	1206:1209	arg1	patterning					1211:1220	stable cell patterning	1199:1220	stable cell patterning	1199:1220	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	0	45	theme	dextran	65:71	arg1	layers					85:90	dextran sacrificial layers	65:90	dextran sacrificial layers	65:90	Metallization and biopatterning on ultra-flexible substrates via dextran sacrificial layers.
25153326	2	46	theme	thin	612:615	arg1	films					617:621	water-soluble dextran sacrificial thin films	578:621	water-soluble dextran sacrificial thin films	578:621	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	5	47	theme	protein	1118:1124	arg1	characteristics					1137:1151	surface wetting and protein adsorption characteristics	1098:1151	characteristics	1137:1151	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	1	48	theme	semiconductor	133:145	arg1	industry					147:154	the semiconductor industry	129:154	the semiconductor industry	129:154	Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors.
25153326	1	49	theme	flexible	371:378	arg1	biosensors					380:389	flexible biosensors	371:389	flexible biosensors	371:389	Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors.
25153326	5	50	theme	stable	1199:1204	arg1	patterning					1211:1220	stable cell patterning	1199:1220	stable cell patterning	1199:1220	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	2	51	theme	sacrificial	600:610	arg1	films					617:621	water-soluble dextran sacrificial thin films	578:621	water-soluble dextran sacrificial thin films	578:621	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	3	52	theme	particle	705:712	arg1	adsorption					714:723	particle adsorption	705:723	particle adsorption	705:723	Dextran films provided a stable template for photolithography, metal deposition, particle adsorption, and protein stamping.
25153326	5	53	theme	straightforward	1045:1059	arg1	approach					1061:1068	this simple and straightforward approach	1029:1068	this simple and straightforward approach	1029:1068	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	1	54	theme	cellular	335:342	arg1	environments					344:355	cellular environments	335:355	cellular environments	335:355	Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors.
25153326	2	55	theme	dextran	592:598	arg1	films					617:621	water-soluble dextran sacrificial thin films	578:621	water-soluble dextran sacrificial thin films	578:621	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	3	56	theme	stable	649:654	arg1	template					656:663	a stable template	647:663	a stable template for photolithography, metal deposition, particle adsorption, and protein stamping	647:745	Dextran films provided a stable template for photolithography, metal deposition, particle adsorption, and protein stamping.
25153326	5	57	theme	simple	1034:1039	arg1	approach					1061:1068	this simple and straightforward approach	1029:1068	this simple and straightforward approach	1029:1068	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	6	58	theme	nano-structures	1522:1536	arg1	host					1492:1495	a host	1490:1495	a host of additional micro- and nano-structures and materials	1490:1550	This method is compatible over the complete moduli range of PDMS, and potentially generalizable over a host of additional micro- and nano-structures and materials.
25153326	4	59	from	water	1002:1006	arg1	etch					979:982	sacrificial etch	967:982	sacrificial etch of the dextran in water	967:1006	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	2	60	theme	water-soluble	578:590	arg1	films					617:621	water-soluble dextran sacrificial thin films	578:621	water-soluble dextran sacrificial thin films	578:621	We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films.
25153326	4	61	theme	dextran	991:997	arg1	etch					979:982	sacrificial etch	967:982	sacrificial etch of the dextran in water	967:1006	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	5	62	theme	protein	1178:1184	arg1	patterns					1186:1193	protein patterns	1178:1193	protein patterns for stable cell patterning	1178:1220	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	6	63	theme	additional	1500:1509	arg1	micro-					1511:1516	additional micro-	1500:1516	additional micro-	1500:1516	This method is compatible over the complete moduli range of PDMS, and potentially generalizable over a host of additional micro- and nano-structures and materials.
25153326	5	64	theme	certain	1335:1341	arg1	metals					1343:1348	certain metals	1335:1348	certain metals with use of vinyl molecular adhesives	1335:1386	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
25153326	4	65	from	dextran	991:997	arg1	water					1002:1006	water	1002:1006	water	1002:1006	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	0	66	theme	sacrificial	73:83	arg1	layers					85:90	dextran sacrificial layers	65:90	dextran sacrificial layers	65:90	Metallization and biopatterning on ultra-flexible substrates via dextran sacrificial layers.
25153326	3	67	theme	protein	730:736	arg1	stamping					738:745	protein stamping	730:745	protein stamping	730:745	Dextran films provided a stable template for photolithography, metal deposition, particle adsorption, and protein stamping.
25153326	4	68	dep	70∶1	884:887	arg1	to					881:882	to	881:882	to	881:882	These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water.
25153326	5	69	theme	cell	1288:1291	arg1	response					1304:1311	cell mechanical response	1288:1311	cell mechanical response	1288:1311	We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives.
27206560	0	0	theme	Oxidized	78:85	arg1	Composite					107:115	Oxidized Corn Starch-Gelatin Composite	78:115	Oxidized Corn Starch-Gelatin Composite	78:115	The Preparation and Applications of One Biodegradable Liquid Film Mulching by Oxidized Corn Starch-Gelatin Composite.
27206560	7	1	theme	germination	1181:1191	arg1	rate					1193:1196	the germination rate	1177:1196	the germination rate of the rapeseed seed	1177:1217	In addition, the germination rate of the rapeseed seed reached 80 %, and the height of rapeseeds obviously increased in pot experiments after adding the liquid film mulching.
27206560	2	2	dep	transform	430:438	arg1	infrared					440:447	infrared	440:447	transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA)	430:554	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	0	3	theme	Starch-Gelatin	92:105	arg1	Composite					107:115	Oxidized Corn Starch-Gelatin Composite	78:115	Oxidized Corn Starch-Gelatin Composite	78:115	The Preparation and Applications of One Biodegradable Liquid Film Mulching by Oxidized Corn Starch-Gelatin Composite.
27206560	6	4	theme	permeability	1150:1161	arg1	properties					1092:1101	excellent properties	1082:1101	excellent properties of water-absorbing and water-retention and low permeability	1082:1161	In the application experiments, the OCS-Gel showed excellent properties of water-absorbing and water-retention and low permeability.
27206560	6	5	theme	water-retention	1126:1140	arg1	properties					1092:1101	excellent properties	1082:1101	excellent properties of water-absorbing and water-retention and low permeability	1082:1161	In the application experiments, the OCS-Gel showed excellent properties of water-absorbing and water-retention and low permeability.
27206560	0	6	theme	Corn	87:90	arg1	Composite					107:115	Oxidized Corn Starch-Gelatin Composite	78:115	Oxidized Corn Starch-Gelatin Composite	78:115	The Preparation and Applications of One Biodegradable Liquid Film Mulching by Oxidized Corn Starch-Gelatin Composite.
27206560	5	7	from	analysis	923:930	arg1	results					895:901	The results	891:901	The results from the structural analysis	891:930	The results from the structural analysis indicated that OCS-Gel enriches pore structure and exhibits high thermal stability up to 324.8 °C.
27206560	7	8	theme	seed	1214:1217	arg1	rate					1193:1196	the germination rate	1177:1196	the germination rate of the rapeseed seed	1177:1217	In addition, the germination rate of the rapeseed seed reached 80 %, and the height of rapeseeds obviously increased in pot experiments after adding the liquid film mulching.
27206560	2	9	theme	electron	482:489	arg1	SEM					503:505	SEM	503:505	SEM	503:505	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	2	9	theme	electron	482:489	arg1	microscope					491:500	a scanning electron microscope	471:500	a scanning electron microscope (SEM) picture	471:514	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	4	10	theme	planting	768:775	arg1	experiments					786:796	the planting rapeseed experiments	764:796	the planting rapeseed experiments	764:796	Finally, the planting rapeseed experiments were carried out, and the germination and growing state of the rapeseed seeds were observed.
27206560	6	11	theme	water-absorbing	1106:1120	arg1	properties					1092:1101	excellent properties	1082:1101	excellent properties of water-absorbing and water-retention and low permeability	1082:1161	In the application experiments, the OCS-Gel showed excellent properties of water-absorbing and water-retention and low permeability.
27206560	2	12	theme	OCS-Gel	366:372	arg1	material					385:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	2	13	theme	scanning	473:480	arg1	SEM					503:505	SEM	503:505	SEM	503:505	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	2	13	theme	scanning	473:480	arg1	microscope					491:500	a scanning electron microscope	471:500	a scanning electron microscope (SEM) picture	471:514	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	3	14	theme	application	646:656	arg1	performances					658:669	the application performances	642:669	the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel	642:738	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	2	15	dep	Fourier	422:428	arg1	transform					430:438	transform	430:438	transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA)	430:554	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	4	16	theme	seeds	870:874	arg1	state					848:852	the germination and growing state	820:852	state	848:852	Finally, the planting rapeseed experiments were carried out, and the germination and growing state of the rapeseed seeds were observed.
27206560	2	17	theme	starch-gelatin	350:363	arg1	material					385:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	6	18	theme	low	1146:1148	arg1	permeability					1150:1161	low permeability	1146:1161	low permeability	1146:1161	In the application experiments, the OCS-Gel showed excellent properties of water-absorbing and water-retention and low permeability.
27206560	1	19	theme	agricultural	243:254	arg1	production					256:265	agricultural production	243:265	agricultural production	243:265	Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research.
27206560	4	20	theme	rapeseed	777:784	arg1	experiments					786:796	the planting rapeseed experiments	764:796	the planting rapeseed experiments	764:796	Finally, the planting rapeseed experiments were carried out, and the germination and growing state of the rapeseed seeds were observed.
27206560	6	21	theme	excellent	1082:1090	arg1	properties					1092:1101	excellent properties	1082:1101	excellent properties of water-absorbing and water-retention and low permeability	1082:1161	In the application experiments, the OCS-Gel showed excellent properties of water-absorbing and water-retention and low permeability.
27206560	2	22	theme	corn	345:348	arg1	material					385:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	3	23	used	used	578:581	arg2	OCS-Gel					561:567	The OCS-Gel	557:567	The OCS-Gel	557:567	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	3	23	used	used	578:581	arg2	mulching					600:607	a liquid film mulching	586:607	a liquid film mulching for agricultural production	586:635	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	7	24	theme	film	1324:1327	arg1	mulching					1329:1336	the liquid film mulching	1313:1336	the liquid film mulching	1313:1336	In addition, the germination rate of the rapeseed seed reached 80 %, and the height of rapeseeds obviously increased in pot experiments after adding the liquid film mulching.
27206560	4	25	theme	growing	840:846	arg1	state					848:852	the germination and growing state	820:852	state	848:852	Finally, the planting rapeseed experiments were carried out, and the germination and growing state of the rapeseed seeds were observed.
27206560	5	26	theme	thermal	997:1003	arg1	stability					1005:1013	high thermal stability	992:1013	high thermal stability up to 324.8 °C	992:1028	The results from the structural analysis indicated that OCS-Gel enriches pore structure and exhibits high thermal stability up to 324.8 °C.
27206560	1	27	theme	abundant	175:182	arg1	starch					159:164	starch	159:164	starch	159:164	Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research.
27206560	1	27	theme	abundant	175:182	arg1	materials					215:223	abundant, recyclable, and biodegradable materials	175:223	abundant, recyclable, and biodegradable materials	175:223	Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research.
27206560	1	27	theme	abundant	175:182	arg1	gelatin					127:133	gelatin	127:133	gelatin (Gel)	127:139	Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research.
27206560	4	28	theme	rapeseed	861:868	arg1	seeds					870:874	the rapeseed seeds	857:874	the rapeseed seeds	857:874	Finally, the planting rapeseed experiments were carried out, and the germination and growing state of the rapeseed seeds were observed.
27206560	3	29	theme	liquid	588:593	arg1	mulching					600:607	a liquid film mulching	586:607	a liquid film mulching for agricultural production	586:635	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	3	29	theme	liquid	588:593	arg1	OCS-Gel					561:567	The OCS-Gel	557:567	The OCS-Gel	557:567	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	2	30	theme	composite	375:383	arg1	material					385:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	7	31	theme	rapeseeds	1251:1259	arg1	height					1241:1246	the height	1237:1246	the height of rapeseeds	1237:1259	In addition, the germination rate of the rapeseed seed reached 80 %, and the height of rapeseeds obviously increased in pot experiments after adding the liquid film mulching.
27206560	7	32	theme	liquid	1317:1322	arg1	mulching					1329:1336	the liquid film mulching	1313:1336	the liquid film mulching	1313:1336	In addition, the germination rate of the rapeseed seed reached 80 %, and the height of rapeseeds obviously increased in pot experiments after adding the liquid film mulching.
27206560	1	33	theme	recyclable	185:194	arg1	starch					159:164	starch	159:164	starch	159:164	Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research.
27206560	1	33	theme	recyclable	185:194	arg1	materials					215:223	abundant, recyclable, and biodegradable materials	175:223	abundant, recyclable, and biodegradable materials	175:223	Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research.
27206560	1	33	theme	recyclable	185:194	arg1	gelatin					127:133	gelatin	127:133	gelatin (Gel)	127:139	Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research.
27206560	0	34	theme	Liquid	54:59	arg1	Mulching					66:73	One Biodegradable Liquid Film Mulching	36:73	One Biodegradable Liquid Film Mulching	36:73	The Preparation and Applications of One Biodegradable Liquid Film Mulching by Oxidized Corn Starch-Gelatin Composite.
27206560	3	35	dep	performances	658:669	arg1	etc.					719:722	etc.	719:722	etc.	719:722	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	3	35	dep	performances	658:669	arg1	permeability					688:699	permeability	688:699	permeability	688:699	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	3	35	dep	performances	658:669	arg1	retention					708:716	water retention	702:716	water retention	702:716	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	3	35	dep	performances	658:669	arg1	hygroscopicity					672:685	hygroscopicity	672:685	hygroscopicity	672:685	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	3	36	theme	water	702:706	arg1	retention					708:716	water retention	702:716	water retention	702:716	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	3	36	theme	water	702:706	arg1	hygroscopicity					672:685	hygroscopicity	672:685	hygroscopicity	672:685	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	0	37	theme	Biodegradable	40:52	arg1	Mulching					66:73	One Biodegradable Liquid Film Mulching	36:73	One Biodegradable Liquid Film Mulching	36:73	The Preparation and Applications of One Biodegradable Liquid Film Mulching by Oxidized Corn Starch-Gelatin Composite.
27206560	2	38	theme	thermogravimetric	523:539	arg1	TGA					551:553	TGA	551:553	TGA	551:553	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	2	38	theme	thermogravimetric	523:539	arg1	analysis					541:548	a thermogravimetric analysis	521:548	a thermogravimetric analysis (TGA)	521:554	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	5	39	theme	pore	964:967	arg1	structure					969:977	pore structure	964:977	pore structure	964:977	The results from the structural analysis indicated that OCS-Gel enriches pore structure and exhibits high thermal stability up to 324.8 °C.
27206560	3	40	theme	agricultural	613:624	arg1	production					626:635	agricultural production	613:635	agricultural production	613:635	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	7	41	theme	pot	1284:1286	arg1	experiments					1288:1298	pot experiments	1284:1298	pot experiments	1284:1298	In addition, the germination rate of the rapeseed seed reached 80 %, and the height of rapeseeds obviously increased in pot experiments after adding the liquid film mulching.
27206560	7	42	theme	rapeseed	1205:1212	arg1	seed					1214:1217	the rapeseed seed	1201:1217	the rapeseed seed	1201:1217	In addition, the germination rate of the rapeseed seed reached 80 %, and the height of rapeseeds obviously increased in pot experiments after adding the liquid film mulching.
27206560	3	43	theme	OCS-Gel	732:738	arg1	performances					658:669	the application performances	642:669	the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel	642:738	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	4	44	theme	germination	824:834	arg1	state					848:852	the germination and growing state	820:852	state	848:852	Finally, the planting rapeseed experiments were carried out, and the germination and growing state of the rapeseed seeds were observed.
27206560	3	45	theme	film	595:598	arg1	mulching					600:607	a liquid film mulching	586:607	a liquid film mulching for agricultural production	586:635	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	3	45	theme	film	595:598	arg1	OCS-Gel					561:567	The OCS-Gel	557:567	The OCS-Gel	557:567	The OCS-Gel was then used as a liquid film mulching for agricultural production, and the application performances (hygroscopicity, permeability, water retention, etc.) of the OCS-Gel were measured.
27206560	5	46	theme	structural	912:921	arg1	analysis					923:930	the structural analysis	908:930	the structural analysis	908:930	The results from the structural analysis indicated that OCS-Gel enriches pore structure and exhibits high thermal stability up to 324.8 °C.
27206560	6	47	theme	application	1038:1048	arg1	experiments					1050:1060	the application experiments	1034:1060	the application experiments	1034:1060	In the application experiments, the OCS-Gel showed excellent properties of water-absorbing and water-retention and low permeability.
27206560	0	48	theme	Mulching	66:73	arg1	Applications					20:31	Applications	20:31	Applications	20:31	The Preparation and Applications of One Biodegradable Liquid Film Mulching by Oxidized Corn Starch-Gelatin Composite.
27206560	0	48	theme	Mulching	66:73	arg1	Preparation					4:14	Preparation	4:14	Preparation	4:14	The Preparation and Applications of One Biodegradable Liquid Film Mulching by Oxidized Corn Starch-Gelatin Composite.
27206560	5	49	theme	high	992:995	arg1	stability					1005:1013	high thermal stability	992:1013	high thermal stability up to 324.8 °C	992:1028	The results from the structural analysis indicated that OCS-Gel enriches pore structure and exhibits high thermal stability up to 324.8 °C.
27206560	0	50	dep	Preparation	4:14	arg1	The					0:2	The	0:2	The	0:2	The Preparation and Applications of One Biodegradable Liquid Film Mulching by Oxidized Corn Starch-Gelatin Composite.
27206560	2	51	theme	oxidized	336:343	arg1	material					385:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	1	52	theme	biodegradable	201:213	arg1	starch					159:164	starch	159:164	starch	159:164	Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research.
27206560	1	52	theme	biodegradable	201:213	arg1	materials					215:223	abundant, recyclable, and biodegradable materials	175:223	abundant, recyclable, and biodegradable materials	175:223	Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research.
27206560	1	52	theme	biodegradable	201:213	arg1	gelatin					127:133	gelatin	127:133	gelatin (Gel)	127:139	Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research.
27206560	0	53	theme	Film	61:64	arg1	Mulching					66:73	One Biodegradable Liquid Film Mulching	36:73	One Biodegradable Liquid Film Mulching	36:73	The Preparation and Applications of One Biodegradable Liquid Film Mulching by Oxidized Corn Starch-Gelatin Composite.
27206560	2	54	theme	prepared	327:334	arg1	material					385:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	the prepared oxidized corn starch-gelatin (OCS-Gel) composite material	323:392	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
27206560	2	55	theme	microscope	491:500	arg1	picture					508:514	a scanning electron microscope (SEM) picture	471:514	a scanning electron microscope (SEM) picture	471:514	Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transform infrared spectrometer (FT-IR), a scanning electron microscope (SEM) picture, and a thermogravimetric analysis (TGA).
24124098	7	0	theme	release	1126:1132	arg1	study					1134:1138	In vitro release study	1117:1138	In vitro release study	1117:1138	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	7	1	theme	latanoprost	1174:1184	arg1	release					1163:1169	longer sustained release	1146:1169	longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels	1146:1247	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	1	2	theme	therapeutic	152:162	arg1	applications					164:175	many therapeutic applications	147:175	many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity	147:263	Hyaluronic acid (HA) is a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity.
24124098	8	3	theme	controlled	1426:1435	arg1	properties					1449:1458	controlled degradation properties	1426:1458	controlled degradation properties	1426:1458	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	8	4	theme	drug	1508:1511	arg1	systems					1522:1528	potential drug delivery systems	1498:1528	potential drug delivery systems for many ocular diseases	1498:1553	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	8	4	theme	drug	1508:1511	arg1	hydrogels					1410:1418	these nanocomposite hydrogels	1390:1418	these nanocomposite hydrogels	1390:1418	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	3	5	theme	sustained	494:502	arg1	release					504:510	localized sustained release	484:510	localized sustained release of drugs inside eye	484:530	However, localized sustained release of drugs inside eye has been difficult to achieve because of the inability to retain carriers for long periods in the eye.
24124098	2	6	from	role	326:329	arg1	healing					340:346	wound healing	334:346	wound healing	334:346	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	8	7	theme	potential	1498:1506	arg1	systems					1522:1528	potential drug delivery systems	1498:1528	potential drug delivery systems for many ocular diseases	1498:1553	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	8	7	theme	potential	1498:1506	arg1	hydrogels					1410:1418	these nanocomposite hydrogels	1390:1418	these nanocomposite hydrogels	1390:1418	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	2	8	theme	eye	298:300	arg1	tissue					302:307	eye tissue	298:307	eye tissue	298:307	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	6	9	theme	nanocomposite	1092:1104	arg1	hydrogels					1106:1114	nanocomposite hydrogels	1092:1114	nanocomposite hydrogels	1092:1114	The HA is first chemically modified, mixed with drug-loaded liposomes, and then crosslinked to obtain nanocomposite hydrogels.
24124098	3	10	from	periods	615:621	arg1	eye					630:632	the eye	626:632	the eye	626:632	However, localized sustained release of drugs inside eye has been difficult to achieve because of the inability to retain carriers for long periods in the eye.
24124098	5	11	theme	latanoprost	933:943	arg1	swelling					876:883	swelling	876:883	swelling	876:883	In order to obtain prolonged sustained delivery, two HA-based composite hydrogels incorporating nanocarriers, have been synthesized and characterized for swelling, rheology, degradation, and in vitro release of latanoprost, a drug used to reduce intraocular pressure.
24124098	5	11	theme	latanoprost	933:943	arg1	release					922:928	in vitro release	913:928	in vitro release of latanoprost, a drug used to reduce intraocular pressure	913:987	In order to obtain prolonged sustained delivery, two HA-based composite hydrogels incorporating nanocarriers, have been synthesized and characterized for swelling, rheology, degradation, and in vitro release of latanoprost, a drug used to reduce intraocular pressure.
24124098	5	11	theme	latanoprost	933:943	arg1	degradation					896:906	degradation	896:906	degradation	896:906	In order to obtain prolonged sustained delivery, two HA-based composite hydrogels incorporating nanocarriers, have been synthesized and characterized for swelling, rheology, degradation, and in vitro release of latanoprost, a drug used to reduce intraocular pressure.
24124098	5	11	theme	latanoprost	933:943	arg1	rheology					886:893	rheology	886:893	rheology	886:893	In order to obtain prolonged sustained delivery, two HA-based composite hydrogels incorporating nanocarriers, have been synthesized and characterized for swelling, rheology, degradation, and in vitro release of latanoprost, a drug used to reduce intraocular pressure.
24124098	2	12	theme	natural	353:359	arg1	HA					266:267	HA	266:267	HA being a natural component of eye tissue with significant role in wound healing	266:346	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	2	12	theme	natural	353:359	arg1	choice					361:366	a natural choice	351:366	a natural choice	351:366	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	7	13	theme	longer	1146:1151	arg1	release					1163:1169	longer sustained release	1146:1169	longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels	1146:1247	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	2	14	with	component	285:293	arg1	role					326:329	significant role	314:329	significant role in wound healing	314:346	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	5	15	theme	in	913:914	arg1	release					922:928	in vitro release	913:928	in vitro release of latanoprost, a drug used to reduce intraocular pressure	913:987	In order to obtain prolonged sustained delivery, two HA-based composite hydrogels incorporating nanocarriers, have been synthesized and characterized for swelling, rheology, degradation, and in vitro release of latanoprost, a drug used to reduce intraocular pressure.
24124098	1	16	theme	Hyaluronic	85:94	arg1	HA					102:103	HA	102:103	HA	102:103	Hyaluronic acid (HA) is a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity.
24124098	1	16	theme	Hyaluronic	85:94	arg1	acid					96:99	Hyaluronic acid	85:99	Hyaluronic acid (HA)	85:104	Hyaluronic acid (HA) is a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity.
24124098	1	16	theme	Hyaluronic	85:94	arg1	biomaterial					131:141	a widely investigated biomaterial	109:141	a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity	109:263	Hyaluronic acid (HA) is a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity.
24124098	2	17	theme	ocular	385:390	arg1	delivery					397:404	ocular drug delivery	385:404	ocular drug delivery	385:404	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	7	18	theme	sustained	1153:1161	arg1	release					1163:1169	longer sustained release	1146:1169	longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels	1146:1247	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	8	19	theme	many	1534:1537	arg1	diseases					1546:1553	many ocular diseases	1534:1553	many ocular diseases	1534:1553	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	0	20	theme	ocular	50:55	arg1	applications					71:82	ocular drug delivery applications	50:82	ocular drug delivery applications	50:82	Hyaluronic acid-based nanocomposite hydrogels for ocular drug delivery applications.
24124098	2	21	theme	natural	277:283	arg1	component					285:293	a natural component	275:293	a natural component of eye tissue with significant role in wound healing	275:346	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	6	22	theme	drug-loaded	1038:1048	arg1	liposomes					1050:1058	drug-loaded liposomes	1038:1058	drug-loaded liposomes	1038:1058	The HA is first chemically modified, mixed with drug-loaded liposomes, and then crosslinked to obtain nanocomposite hydrogels.
24124098	8	23	with	hydrogels	1410:1418	arg1	properties					1449:1458	controlled degradation properties	1426:1458	controlled degradation properties	1426:1458	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	8	23	with	hydrogels	1410:1418	arg1	release					1474:1480	sustained release	1464:1480	sustained release	1464:1480	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	8	24	theme	delivery	1513:1520	arg1	systems					1522:1528	potential drug delivery systems	1498:1528	potential drug delivery systems for many ocular diseases	1498:1553	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	8	24	theme	delivery	1513:1520	arg1	hydrogels					1410:1418	these nanocomposite hydrogels	1390:1418	these nanocomposite hydrogels	1390:1418	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	4	25	theme	noncrosslinked	641:654	arg1	HA					666:667	noncrosslinked (soluble) HA	641:667	noncrosslinked (soluble) HA	641:667	Using noncrosslinked (soluble) HA offers limited control over site retention of drugs.
24124098	7	26	dep	In	1117:1118	arg1	vitro					1120:1124	vitro	1120:1124	vitro	1120:1124	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	2	27	theme	significant	314:324	arg1	role					326:329	significant role	314:329	significant role in wound healing	314:346	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	7	28	dep	hydrogels	1201:1209	arg1	compared					1214:1221	compared	1214:1221	compared to liposomes or hydrogels	1214:1247	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	1	29	theme	unique	190:195	arg1	properties					197:206	its unique properties	186:206	its unique properties of biocompatibility, biodegradation, and viscoelasticity	186:263	Hyaluronic acid (HA) is a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity.
24124098	2	30	theme	incorporated	420:431	arg1	drugs					433:437	the incorporated drugs	416:437	the incorporated drugs	416:437	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	8	31	theme	ocular	1539:1544	arg1	diseases					1546:1553	many ocular diseases	1534:1553	many ocular diseases	1534:1553	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	4	32	theme	drugs	715:719	arg1	retention					702:710	site retention	697:710	site retention of drugs	697:719	Using noncrosslinked (soluble) HA offers limited control over site retention of drugs.
24124098	5	33	theme	intraocular	968:978	arg1	pressure					980:987	intraocular pressure	968:987	intraocular pressure	968:987	In order to obtain prolonged sustained delivery, two HA-based composite hydrogels incorporating nanocarriers, have been synthesized and characterized for swelling, rheology, degradation, and in vitro release of latanoprost, a drug used to reduce intraocular pressure.
24124098	4	34	theme	site	697:700	arg1	retention					702:710	site retention	697:710	site retention of drugs	697:719	Using noncrosslinked (soluble) HA offers limited control over site retention of drugs.
24124098	3	35	theme	drugs	515:519	arg1	release					504:510	localized sustained release	484:510	localized sustained release of drugs inside eye	484:530	However, localized sustained release of drugs inside eye has been difficult to achieve because of the inability to retain carriers for long periods in the eye.
24124098	8	36	theme	degradation	1437:1447	arg1	properties					1449:1458	controlled degradation properties	1426:1458	controlled degradation properties	1426:1458	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	2	37	theme	tissue	302:307	arg1	component					285:293	a natural component	275:293	a natural component of eye tissue with significant role in wound healing	275:346	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	7	38	theme	drug	1291:1294	arg1	diffusion					1296:1304	drug diffusion	1291:1304	drug diffusion	1291:1304	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	2	39	theme	sustained	457:465	arg1	manner					467:472	a sustained manner	455:472	a sustained manner	455:472	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	7	40	theme	additional	1266:1275	arg1	resistance					1277:1286	additional resistance	1266:1286	additional resistance to drug diffusion	1266:1304	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	4	41	link	noncrosslinked	641:654	arg1	HA					666:667	noncrosslinked (soluble) HA	641:667	noncrosslinked (soluble) HA	641:667	Using noncrosslinked (soluble) HA offers limited control over site retention of drugs.
24124098	1	42	theme	biocompatibility	211:226	arg1	properties					197:206	its unique properties	186:206	its unique properties of biocompatibility, biodegradation, and viscoelasticity	186:263	Hyaluronic acid (HA) is a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity.
24124098	5	43	theme	prolonged	741:749	arg1	delivery					761:768	prolonged sustained delivery	741:768	prolonged sustained delivery	741:768	In order to obtain prolonged sustained delivery, two HA-based composite hydrogels incorporating nanocarriers, have been synthesized and characterized for swelling, rheology, degradation, and in vitro release of latanoprost, a drug used to reduce intraocular pressure.
24124098	7	44	theme	In	1117:1118	arg1	study					1134:1138	In vitro release study	1117:1138	In vitro release study	1117:1138	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	3	45	theme	long	610:613	arg1	periods					615:621	long periods	610:621	long periods in the eye	610:632	However, localized sustained release of drugs inside eye has been difficult to achieve because of the inability to retain carriers for long periods in the eye.
24124098	5	46	theme	HA-based	775:782	arg1	hydrogels					794:802	two HA-based composite hydrogels	771:802	two HA-based composite hydrogels	771:802	In order to obtain prolonged sustained delivery, two HA-based composite hydrogels incorporating nanocarriers, have been synthesized and characterized for swelling, rheology, degradation, and in vitro release of latanoprost, a drug used to reduce intraocular pressure.
24124098	2	47	theme	drug	392:395	arg1	delivery					397:404	ocular drug delivery	385:404	ocular drug delivery	385:404	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	0	48	theme	delivery	62:69	arg1	applications					71:82	ocular drug delivery applications	50:82	ocular drug delivery applications	50:82	Hyaluronic acid-based nanocomposite hydrogels for ocular drug delivery applications.
24124098	3	49	theme	localized	484:492	arg1	release					504:510	localized sustained release	484:510	localized sustained release of drugs inside eye	484:530	However, localized sustained release of drugs inside eye has been difficult to achieve because of the inability to retain carriers for long periods in the eye.
24124098	4	50	theme	limited	676:682	arg1	control					684:690	limited control	676:690	limited control	676:690	Using noncrosslinked (soluble) HA offers limited control over site retention of drugs.
24124098	0	51	theme	drug	57:60	arg1	applications					71:82	ocular drug delivery applications	50:82	ocular drug delivery applications	50:82	Hyaluronic acid-based nanocomposite hydrogels for ocular drug delivery applications.
24124098	5	52	dep	in	913:914	arg1	vitro					916:920	vitro	916:920	vitro	916:920	In order to obtain prolonged sustained delivery, two HA-based composite hydrogels incorporating nanocarriers, have been synthesized and characterized for swelling, rheology, degradation, and in vitro release of latanoprost, a drug used to reduce intraocular pressure.
24124098	8	53	theme	sustained	1464:1472	arg1	release					1474:1480	sustained release	1464:1480	sustained release	1464:1480	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	7	54	theme	liposomes	1338:1346	arg1	incorporation					1321:1333	the incorporation	1317:1333	the incorporation of liposomes inside the hydrogels	1317:1367	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	1	55	theme	investigated	118:129	arg1	biomaterial					131:141	a widely investigated biomaterial	109:141	a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity	109:263	Hyaluronic acid (HA) is a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity.
24124098	1	55	theme	investigated	118:129	arg1	acid					96:99	Hyaluronic acid	85:99	Hyaluronic acid (HA)	85:104	Hyaluronic acid (HA) is a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity.
24124098	1	56	theme	biodegradation	229:242	arg1	properties					197:206	its unique properties	186:206	its unique properties of biocompatibility, biodegradation, and viscoelasticity	186:263	Hyaluronic acid (HA) is a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity.
24124098	8	57	theme	nanocomposite	1396:1408	arg1	hydrogels					1410:1418	these nanocomposite hydrogels	1390:1418	these nanocomposite hydrogels	1390:1418	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	8	57	theme	nanocomposite	1396:1408	arg1	systems					1522:1528	potential drug delivery systems	1498:1528	potential drug delivery systems for many ocular diseases	1498:1553	It is believed that these nanocomposite hydrogels, with controlled degradation properties and sustained release, could serve as potential drug delivery systems for many ocular diseases.
24124098	2	58	theme	wound	334:338	arg1	healing					340:346	wound healing	334:346	wound healing	334:346	HA being a natural component of eye tissue with significant role in wound healing is a natural choice as a carrier for ocular drug delivery, provided the incorporated drugs are released in a sustained manner.
24124098	7	59	theme	composite	1191:1199	arg1	hydrogels					1201:1209	composite hydrogels	1191:1209	composite hydrogels as compared to liposomes or hydrogels	1191:1247	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	7	60	from	hydrogels	1201:1209	arg1	release					1163:1169	longer sustained release	1146:1169	longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels	1146:1247	In vitro release study shows longer sustained release of latanoprost from composite hydrogels as compared to liposomes or hydrogels alone indicating additional resistance to drug diffusion because of the incorporation of liposomes inside the hydrogels.
24124098	4	61	theme	soluble	657:663	arg1	HA					666:667	noncrosslinked (soluble) HA	641:667	noncrosslinked (soluble) HA	641:667	Using noncrosslinked (soluble) HA offers limited control over site retention of drugs.
24124098	5	62	theme	sustained	751:759	arg1	delivery					761:768	prolonged sustained delivery	741:768	prolonged sustained delivery	741:768	In order to obtain prolonged sustained delivery, two HA-based composite hydrogels incorporating nanocarriers, have been synthesized and characterized for swelling, rheology, degradation, and in vitro release of latanoprost, a drug used to reduce intraocular pressure.
24124098	5	63	theme	composite	784:792	arg1	hydrogels					794:802	two HA-based composite hydrogels	771:802	two HA-based composite hydrogels	771:802	In order to obtain prolonged sustained delivery, two HA-based composite hydrogels incorporating nanocarriers, have been synthesized and characterized for swelling, rheology, degradation, and in vitro release of latanoprost, a drug used to reduce intraocular pressure.
24124098	1	64	theme	many	147:150	arg1	applications					164:175	many therapeutic applications	147:175	many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity	147:263	Hyaluronic acid (HA) is a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity.
24124098	1	65	theme	viscoelasticity	249:263	arg1	properties					197:206	its unique properties	186:206	its unique properties of biocompatibility, biodegradation, and viscoelasticity	186:263	Hyaluronic acid (HA) is a widely investigated biomaterial for many therapeutic applications owing to its unique properties of biocompatibility, biodegradation, and viscoelasticity.
25821851	1	0	theme	cells	355:359	arg1	differentiation					290:304	osteogenic differentiation	279:304	osteogenic differentiation	279:304	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	1	0	theme	cells	355:359	arg1	proliferation					261:273	proliferation	261:273	proliferation	261:273	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	1	1	theme	composite	238:246	arg1	scaffold					248:255	a composite scaffold	236:255	a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells	236:359	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	1	1	theme	composite	238:246	arg1	performance					144:154	the in vitro performance	131:154	the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan	131:231	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	4	2	with	sponges	681:687	arg1	plasma					722:727	the activated platelet-rich plasma	694:727	the activated platelet-rich plasma	694:727	The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma.
25821851	6	3	dep	properties	1058:1067	arg1	the					1023:1025	the	1023:1025	the	1023:1025	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	6	4	dep	%	1098:1098	arg1	w/v					1101:1103	w/v	1101:1103	w/v	1101:1103	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	5	5	theme	stem	930:933	arg1	cells					935:939	the stem cells	926:939	the stem cells	926:939	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	6	6	theme	preparation	951:961	arg1	conditions					963:972	The best preparation conditions	942:972	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation	942:1090	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	6	6	theme	preparation	951:961	arg1	chitosan					1106:1113	3% (w/v) chitosan	1097:1113	3% (w/v) chitosan	1097:1113	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	0	7	theme	regenerative	79:90	arg1	medicine					92:99	regenerative medicine	79:99	regenerative medicine	79:99	Performance of PRP associated with porous chitosan as a composite scaffold for regenerative medicine.
25821851	6	8	theme	-20°C	1121:1125	arg1	temperature					1136:1146	a -20°C freezing temperature	1119:1146	a -20°C freezing temperature	1119:1146	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	5	9	theme	cells	935:939	arg1	release					869:875	the release	865:875	the release of growth factors	865:893	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	5	9	theme	cells	935:939	arg1	proliferation					909:921	increased proliferation	899:921	increased proliferation of the stem cells	899:939	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	3	10	theme	platelet-rich	497:509	arg1	plasma					511:516	The platelet-rich plasma	493:516	The platelet-rich plasma	493:516	The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum.
25821851	6	11	theme	best	946:949	arg1	conditions					963:972	The best preparation conditions	942:972	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation	942:1090	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	6	11	theme	best	946:949	arg1	chitosan					1106:1113	3% (w/v) chitosan	1097:1113	3% (w/v) chitosan	1097:1113	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	6	12	theme	physicochemical	1027:1041	arg1	properties					1058:1067	properties	1058:1067	properties	1058:1067	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	6	13	theme	cell	1073:1076	arg1	proliferation					1078:1090	cell proliferation	1073:1090	cell proliferation	1073:1090	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	5	14	theme	increased	899:907	arg1	proliferation					909:921	increased proliferation	899:921	increased proliferation of the stem cells	899:939	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	1	15	theme	activated	159:167	arg1	plasma					183:188	activated platelet-rich plasma	159:188	activated platelet-rich plasma associated with porous sponges of chitosan	159:231	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	5	16	from	superior	786:793	arg1	terms					847:851	terms	847:851	terms of delaying the release of growth factors and increased proliferation of the stem cells	847:939	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	7	17	theme	regenerative	1249:1260	arg1	medicine					1262:1269	regenerative medicine	1249:1269	regenerative medicine	1249:1269	Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days.
25821851	4	18	theme	platelet-rich	708:720	arg1	plasma					722:727	the activated platelet-rich plasma	694:727	the activated platelet-rich plasma	694:727	The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma.
25821851	1	19	theme	platelet-rich	169:181	arg1	plasma					183:188	activated platelet-rich plasma	159:188	activated platelet-rich plasma associated with porous sponges of chitosan	159:231	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	6	20	theme	osteogenic	1170:1179	arg1	differentiation					1181:1195	osteogenic differentiation	1170:1195	osteogenic differentiation	1170:1195	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	4	21	theme	activated	698:706	arg1	plasma					722:727	the activated platelet-rich plasma	694:727	the activated platelet-rich plasma	694:727	The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma.
25821851	6	22	theme	freezing	1127:1134	arg1	temperature					1136:1146	a -20°C freezing temperature	1119:1146	a -20°C freezing temperature	1119:1146	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	1	23	theme	plasma	183:188	arg1	scaffold					248:255	a composite scaffold	236:255	a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells	236:359	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	1	23	theme	plasma	183:188	arg1	performance					144:154	the in vitro performance	131:154	the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan	131:231	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	1	24	theme	osteogenic	279:288	arg1	differentiation					290:304	osteogenic differentiation	279:304	osteogenic differentiation	279:304	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	5	25	dep	showed	742:747	arg1	superior					786:793	superior	786:793	superior	786:793	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	0	26	theme	PRP	15:17	arg1	Performance					0:10	Performance	0:10	Performance of PRP	0:17	Performance of PRP associated with porous chitosan as a composite scaffold for regenerative medicine.
25821851	3	27	theme	autologous	608:617	arg1	serum					619:623	autologous serum	608:623	autologous serum	608:623	The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum.
25821851	1	28	dep	in	135:136	arg1	vitro					138:142	vitro	138:142	vitro	138:142	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	5	29	theme	activated	806:814	arg1	plasma					830:835	activated platelet-rich plasma	806:835	activated platelet-rich plasma alone	806:841	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	0	30	theme	porous	35:40	arg1	chitosan					42:49	porous chitosan	35:49	porous chitosan	35:49	Performance of PRP associated with porous chitosan as a composite scaffold for regenerative medicine.
25821851	5	31	theme	growth	880:885	arg1	factors					887:893	growth factors	880:893	growth factors	880:893	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	5	32	theme	platelet-rich	816:828	arg1	plasma					830:835	activated platelet-rich plasma	806:835	activated platelet-rich plasma alone	806:841	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	2	33	theme	controlled	391:400	arg1	freezing					402:409	controlled freezing	391:409	controlled freezing (-20, -80, or -196°C)	391:431	The sponges were prepared by controlled freezing (-20, -80, or -196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v).
25821851	1	34	theme	porous	206:211	arg1	sponges					213:219	porous sponges	206:219	porous sponges of chitosan	206:231	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	2	35	theme	solutions	464:472	arg1	lyophilization					437:450	lyophilization	437:450	lyophilization of chitosan solutions (1, 2, or 3% w/v)	437:490	The sponges were prepared by controlled freezing (-20, -80, or -196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v).
25821851	2	35	theme	solutions	464:472	arg1	freezing					402:409	controlled freezing	391:409	controlled freezing (-20, -80, or -196°C)	391:431	The sponges were prepared by controlled freezing (-20, -80, or -196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v).
25821851	2	35	theme	solutions	464:472	arg1	w/v					487:489	1, 2, or 3% w/v	475:489	1, 2, or 3% w/v	475:489	The sponges were prepared by controlled freezing (-20, -80, or -196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v).
25821851	1	36	theme	human	309:313	arg1	cells					355:359	human adipose tissue-derived mesenchymal stem cells	309:359	human adipose tissue-derived mesenchymal stem cells	309:359	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	3	37	theme	whole	565:569	arg1	blood					571:575	whole blood	565:575	whole blood	565:575	The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum.
25821851	1	38	link	tissue-derived	323:336	arg1	cells					355:359	human adipose tissue-derived mesenchymal stem cells	309:359	human adipose tissue-derived mesenchymal stem cells	309:359	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	6	39	theme	coordinated	1011:1021	arg1	properties					1058:1067	properties	1058:1067	properties	1058:1067	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	2	40	theme	chitosan	455:462	arg1	solutions					464:472	chitosan solutions	455:472	chitosan solutions	455:472	The sponges were prepared by controlled freezing (-20, -80, or -196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v).
25821851	1	41	theme	adipose	315:321	arg1	cells					355:359	human adipose tissue-derived mesenchymal stem cells	309:359	human adipose tissue-derived mesenchymal stem cells	309:359	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	3	42	theme	controlled	536:545	arg1	centrifugation					547:560	controlled centrifugation	536:560	controlled centrifugation of whole blood	536:575	The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum.
25821851	5	43	theme	scaffolds	772:780	arg1	performance					753:763	the performance	749:763	the performance of the scaffolds	749:780	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	1	44	theme	tissue-derived	323:336	arg1	cells					355:359	human adipose tissue-derived mesenchymal stem cells	309:359	human adipose tissue-derived mesenchymal stem cells	309:359	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	7	45	theme	composite	1211:1219	arg1	promising					1235:1243	promising	1235:1243	promising	1235:1243	Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days.
25821851	7	45	theme	composite	1211:1219	arg1	scaffolds					1221:1229	the composite scaffolds	1207:1229	the composite scaffolds	1207:1229	Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days.
25821851	4	46	theme	composite	630:638	arg1	scaffolds					640:648	The composite scaffolds	626:648	The composite scaffolds	626:648	The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma.
25821851	1	47	theme	mesenchymal	338:348	arg1	cells					355:359	human adipose tissue-derived mesenchymal stem cells	309:359	human adipose tissue-derived mesenchymal stem cells	309:359	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	2	48	dep	freezing	402:409	arg1	-80					417:419	-80	417:419	-80	417:419	The sponges were prepared by controlled freezing (-20, -80, or -196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v).
25821851	2	48	dep	freezing	402:409	arg1	-196°C					425:430	-196°C	425:430	-196°C	425:430	The sponges were prepared by controlled freezing (-20, -80, or -196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v).
25821851	6	49	theme	scaffolds	996:1004	arg1	conditions					963:972	The best preparation conditions	942:972	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation	942:1090	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	6	49	theme	scaffolds	996:1004	arg1	chitosan					1106:1113	3% (w/v) chitosan	1097:1113	3% (w/v) chitosan	1097:1113	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	5	50	theme	factors	887:893	arg1	release					869:875	the release	865:875	the release of growth factors	865:893	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	5	50	theme	factors	887:893	arg1	proliferation					909:921	increased proliferation	899:921	increased proliferation of the stem cells	899:939	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	6	51	theme	mechanical	1047:1056	arg1	properties					1058:1067	properties	1058:1067	properties	1058:1067	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	6	52	theme	3	1097:1097	arg1	%					1098:1098	%	1098:1098	%	1098:1098	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	1	53	theme	chitosan	224:231	arg1	sponges					213:219	porous sponges	206:219	porous sponges of chitosan	206:231	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	0	54	theme	composite	56:64	arg1	scaffold					66:73	a composite scaffold	54:73	a composite scaffold for regenerative medicine	54:99	Performance of PRP associated with porous chitosan as a composite scaffold for regenerative medicine.
25821851	3	55	theme	blood	571:575	arg1	centrifugation					547:560	controlled centrifugation	536:560	controlled centrifugation of whole blood	536:575	The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum.
25821851	5	56	from	terms	847:851	arg1	superior					786:793	superior	786:793	superior	786:793	The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
25821851	6	57	theme	%	1098:1098	arg1	conditions					963:972	The best preparation conditions	942:972	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation	942:1090	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	6	57	theme	%	1098:1098	arg1	chitosan					1106:1113	3% (w/v) chitosan	1097:1113	3% (w/v) chitosan	1097:1113	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	6	58	theme	composite	986:994	arg1	scaffolds					996:1004	chitosan composite scaffolds	977:1004	chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation	977:1090	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	1	59	theme	stem	350:353	arg1	cells					355:359	human adipose tissue-derived mesenchymal stem cells	309:359	human adipose tissue-derived mesenchymal stem cells	309:359	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	6	60	theme	chitosan	977:984	arg1	scaffolds					996:1004	chitosan composite scaffolds	977:1004	chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation	977:1090	The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation.
25821851	1	61	theme	in	135:136	arg1	scaffold					248:255	a composite scaffold	236:255	a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells	236:359	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	1	61	theme	in	135:136	arg1	performance					144:154	the in vitro performance	131:154	the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan	131:231	This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
25821851	2	62	theme	%	485:485	arg1	lyophilization					437:450	lyophilization	437:450	lyophilization of chitosan solutions (1, 2, or 3% w/v)	437:490	The sponges were prepared by controlled freezing (-20, -80, or -196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v).
25821851	2	62	theme	%	485:485	arg1	w/v					487:489	1, 2, or 3% w/v	475:489	1, 2, or 3% w/v	475:489	The sponges were prepared by controlled freezing (-20, -80, or -196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v).
25877400	5	0	theme	crystallinity	828:840	arg1	increase					859:866	and the crystallinity and surface area increase	820:866	increase	859:866	With different carbon sources, the crystalline structure does not change and the crystallinity and surface area increase.
25877400	0	1	from	Effect	0:5	arg1	Performance					42:52	the Catalytic Performance	28:52	the Catalytic Performance of Ni/β-Mo2C	28:65	Effect of Carbon Sources on the Catalytic Performance of Ni/β-Mo2C.
25877400	1	2	from	catalysts	252:260	arg1	MFCs					299:302	MFCs	299:302	MFCs	299:302	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	2	from	catalysts	252:260	arg1	cells					292:296	air-cathode microbial fuel cells	265:296	air-cathode microbial fuel cells (MFCs)	265:303	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	5	3	theme	crystalline	782:792	arg1	structure					794:802	the crystalline structure	778:802	the crystalline structure	778:802	With different carbon sources, the crystalline structure does not change and the crystallinity and surface area increase.
25877400	2	4	theme	electron	394:401	arg1	microscope					403:412	scanning electron microscope	385:412	scanning electron microscope (SEM)	385:418	The carburized catalyst samples were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and Brunauer-Emmett-Teller (BET).
25877400	2	4	theme	electron	394:401	arg1	SEM					415:417	SEM	415:417	SEM	415:417	The carburized catalyst samples were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and Brunauer-Emmett-Teller (BET).
25877400	3	5	theme	Klebsiella	624:633	arg1	conditions					646:655	Klebsiella pneumoniae conditions	624:655	Klebsiella pneumoniae conditions	624:655	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	1	6	link	solution-derived	132:147	arg1	precursor					149:157	solution-derived precursor	132:157	solution-derived precursor with two different carbon sources (starch and glucose)	132:212	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	6	7	theme	bio-	946:949	arg1	performance					972:982	the better bio- and electrocatalytic performance	935:982	the better bio- and electrocatalytic performance than Ni/β-Mo2C(S)	935:1000	The electrocatalytic experiments show that the Ni/β-Mo2C(G) gives the better bio- and electrocatalytic performance than Ni/β-Mo2C(S) due to its higher crystallinity and BET surface area.
25877400	2	8	theme	scanning	385:392	arg1	microscope					403:412	scanning electron microscope	385:412	scanning electron microscope (SEM)	385:418	The carburized catalyst samples were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and Brunauer-Emmett-Teller (BET).
25877400	2	8	theme	scanning	385:392	arg1	SEM					415:417	SEM	415:417	SEM	415:417	The carburized catalyst samples were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and Brunauer-Emmett-Teller (BET).
25877400	4	9	theme	composite	662:670	arg1	anodes					676:681	The composite MFC anodes	658:681	The composite MFC anodes	658:681	The composite MFC anodes were fabricated, and their catalytic behavior was investigated.
25877400	2	10	theme	catalyst	321:328	arg1	samples					330:336	The carburized catalyst samples	306:336	The carburized catalyst samples	306:336	The carburized catalyst samples were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and Brunauer-Emmett-Teller (BET).
25877400	0	11	theme	Sources	17:23	arg1	Effect					0:5	Effect	0:5	Effect of Carbon Sources on the Catalytic Performance of Ni/β-Mo2C.	0:66	Effect of Carbon Sources on the Catalytic Performance of Ni/β-Mo2C.
25877400	3	12	theme	electrocatalyst	475:489	arg1	activity					459:466	The activity	455:466	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol)	455:593	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	5	13	theme	carbon	762:767	arg1	sources					769:775	different carbon sources	752:775	different carbon sources	752:775	With different carbon sources, the crystalline structure does not change and the crystallinity and surface area increase.
25877400	0	14	theme	Carbon	10:15	arg1	Sources					17:23	Carbon Sources	10:23	Carbon Sources	10:23	Effect of Carbon Sources on the Catalytic Performance of Ni/β-Mo2C.
25877400	1	15	theme	anodic	228:233	arg1	catalysts					252:260	anodic noble-metal-free catalysts	228:260	anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs)	228:303	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	15	theme	anodic	228:233	arg1	Ni/β-Mo2C					83:91	Ni/β-Mo2C	83:91	Ni/β-Mo2C(S)	83:94	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	4	16	theme	MFC	672:674	arg1	anodes					676:681	The composite MFC anodes	658:681	The composite MFC anodes	658:681	The composite MFC anodes were fabricated, and their catalytic behavior was investigated.
25877400	3	17	dep	products	554:561	arg1	products					554:561	several common microbial fermentation products	516:561	several common microbial fermentation products (formate, lactate, and ethanol)	516:593	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	17	dep	products	554:561	arg1	lactate					573:579	lactate	573:579	lactate	573:579	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	17	dep	products	554:561	arg1	ethanol					586:592	ethanol	586:592	ethanol	586:592	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	17	dep	products	554:561	arg1	formate					564:570	formate	564:570	formate	564:570	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	1	18	theme	noble-metal-free	235:250	arg1	catalysts					252:260	anodic noble-metal-free catalysts	228:260	anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs)	228:303	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	18	theme	noble-metal-free	235:250	arg1	Ni/β-Mo2C					83:91	Ni/β-Mo2C	83:91	Ni/β-Mo2C(S)	83:94	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	19	with	precursor	149:157	arg1	glucose					205:211	glucose	205:211	glucose	205:211	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	19	with	precursor	149:157	arg1	starch					194:199	starch	194:199	starch	194:199	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	19	with	precursor	149:157	arg1	sources					185:191	two different carbon sources	164:191	two different carbon sources (starch and glucose)	164:212	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	6	20	theme	surface	1042:1048	arg1	area					1050:1053	BET surface area	1038:1053	BET surface area	1038:1053	The electrocatalytic experiments show that the Ni/β-Mo2C(G) gives the better bio- and electrocatalytic performance than Ni/β-Mo2C(S) due to its higher crystallinity and BET surface area.
25877400	3	21	theme	common	524:529	arg1	products					554:561	several common microbial fermentation products	516:561	several common microbial fermentation products (formate, lactate, and ethanol)	516:593	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	21	theme	common	524:529	arg1	lactate					573:579	lactate	573:579	lactate	573:579	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	21	theme	common	524:529	arg1	ethanol					586:592	ethanol	586:592	ethanol	586:592	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	21	theme	common	524:529	arg1	formate					564:570	formate	564:570	formate	564:570	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	1	22	theme	solution-derived	132:147	arg1	precursor					149:157	solution-derived precursor	132:157	solution-derived precursor with two different carbon sources (starch and glucose)	132:212	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	2	23	theme	X-ray	360:364	arg1	XRD					379:381	XRD	379:381	XRD	379:381	The carburized catalyst samples were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and Brunauer-Emmett-Teller (BET).
25877400	2	23	theme	X-ray	360:364	arg1	diffraction					366:376	X-ray diffraction	360:376	X-ray diffraction (XRD)	360:382	The carburized catalyst samples were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and Brunauer-Emmett-Teller (BET).
25877400	3	24	dep	Klebsiella	624:633	arg1	pneumoniae					635:644	pneumoniae	635:644	pneumoniae	635:644	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	25	theme	microbial	531:539	arg1	products					554:561	several common microbial fermentation products	516:561	several common microbial fermentation products (formate, lactate, and ethanol)	516:593	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	25	theme	microbial	531:539	arg1	lactate					573:579	lactate	573:579	lactate	573:579	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	25	theme	microbial	531:539	arg1	ethanol					586:592	ethanol	586:592	ethanol	586:592	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	25	theme	microbial	531:539	arg1	formate					564:570	formate	564:570	formate	564:570	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	1	26	theme	different	168:176	arg1	glucose					205:211	glucose	205:211	glucose	205:211	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	26	theme	different	168:176	arg1	starch					194:199	starch	194:199	starch	194:199	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	26	theme	different	168:176	arg1	sources					185:191	two different carbon sources	164:191	two different carbon sources (starch and glucose)	164:212	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	6	27	theme	electrocatalytic	873:888	arg1	experiments					890:900	The electrocatalytic experiments	869:900	The electrocatalytic experiments	869:900	The electrocatalytic experiments show that the Ni/β-Mo2C(G) gives the better bio- and electrocatalytic performance than Ni/β-Mo2C(S) due to its higher crystallinity and BET surface area.
25877400	1	28	dep	sources	185:191	arg1	glucose					205:211	glucose	205:211	glucose	205:211	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	28	dep	sources	185:191	arg1	starch					194:199	starch	194:199	starch	194:199	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	28	dep	sources	185:191	arg1	sources					185:191	two different carbon sources	164:191	two different carbon sources (starch and glucose)	164:212	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	0	29	theme	Catalytic	32:40	arg1	Performance					42:52	the Catalytic Performance	28:52	the Catalytic Performance of Ni/β-Mo2C	28:65	Effect of Carbon Sources on the Catalytic Performance of Ni/β-Mo2C.
25877400	1	30	theme	air-cathode	265:275	arg1	MFCs					299:302	MFCs	299:302	MFCs	299:302	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	30	theme	air-cathode	265:275	arg1	cells					292:296	air-cathode microbial fuel cells	265:296	air-cathode microbial fuel cells (MFCs)	265:303	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	3	31	theme	several	516:522	arg1	products					554:561	several common microbial fermentation products	516:561	several common microbial fermentation products (formate, lactate, and ethanol)	516:593	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	31	theme	several	516:522	arg1	lactate					573:579	lactate	573:579	lactate	573:579	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	31	theme	several	516:522	arg1	ethanol					586:592	ethanol	586:592	ethanol	586:592	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	31	theme	several	516:522	arg1	formate					564:570	formate	564:570	formate	564:570	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	5	32	theme	surface	846:852	arg1	area					854:857	surface area	846:857	surface area	846:857	With different carbon sources, the crystalline structure does not change and the crystallinity and surface area increase.
25877400	1	33	theme	microbial	277:285	arg1	MFCs					299:302	MFCs	299:302	MFCs	299:302	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	33	theme	microbial	277:285	arg1	cells					292:296	air-cathode microbial fuel cells	265:296	air-cathode microbial fuel cells (MFCs)	265:303	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	4	34	theme	catalytic	710:718	arg1	behavior					720:727	their catalytic behavior	704:727	their catalytic behavior	704:727	The composite MFC anodes were fabricated, and their catalytic behavior was investigated.
25877400	5	35	theme	area	854:857	arg1	increase					859:866	and the crystallinity and surface area increase	820:866	increase	859:866	With different carbon sources, the crystalline structure does not change and the crystallinity and surface area increase.
25877400	1	36	theme	carbon	178:183	arg1	glucose					205:211	glucose	205:211	glucose	205:211	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	36	theme	carbon	178:183	arg1	starch					194:199	starch	194:199	starch	194:199	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	36	theme	carbon	178:183	arg1	sources					185:191	two different carbon sources	164:191	two different carbon sources (starch and glucose)	164:212	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	5	37	theme	different	752:760	arg1	sources					769:775	different carbon sources	752:775	different carbon sources	752:775	With different carbon sources, the crystalline structure does not change and the crystallinity and surface area increase.
25877400	6	38	theme	BET	1038:1040	arg1	area					1050:1053	BET surface area	1038:1053	BET surface area	1038:1053	The electrocatalytic experiments show that the Ni/β-Mo2C(G) gives the better bio- and electrocatalytic performance than Ni/β-Mo2C(S) due to its higher crystallinity and BET surface area.
25877400	0	39	theme	Ni/β-Mo2C	57:65	arg1	Performance					42:52	the Catalytic Performance	28:52	the Catalytic Performance of Ni/β-Mo2C	28:65	Effect of Carbon Sources on the Catalytic Performance of Ni/β-Mo2C.
25877400	6	40	theme	electrocatalytic	955:970	arg1	performance					972:982	the better bio- and electrocatalytic performance	935:982	the better bio- and electrocatalytic performance than Ni/β-Mo2C(S)	935:1000	The electrocatalytic experiments show that the Ni/β-Mo2C(G) gives the better bio- and electrocatalytic performance than Ni/β-Mo2C(S) due to its higher crystallinity and BET surface area.
25877400	3	41	theme	fermentation	541:552	arg1	products					554:561	several common microbial fermentation products	516:561	several common microbial fermentation products (formate, lactate, and ethanol)	516:593	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	41	theme	fermentation	541:552	arg1	lactate					573:579	lactate	573:579	lactate	573:579	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	41	theme	fermentation	541:552	arg1	ethanol					586:592	ethanol	586:592	ethanol	586:592	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	3	41	theme	fermentation	541:552	arg1	formate					564:570	formate	564:570	formate	564:570	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	6	42	theme	Ni/β-Mo2C	989:997	arg1	S					999:999	Ni/β-Mo2C(S)	989:1000	Ni/β-Mo2C(S)	989:1000	The electrocatalytic experiments show that the Ni/β-Mo2C(G) gives the better bio- and electrocatalytic performance than Ni/β-Mo2C(S) due to its higher crystallinity and BET surface area.
25877400	2	43	theme	carburized	310:319	arg1	samples					330:336	The carburized catalyst samples	306:336	The carburized catalyst samples	306:336	The carburized catalyst samples were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and Brunauer-Emmett-Teller (BET).
25877400	1	44	theme	fuel	287:290	arg1	MFCs					299:302	MFCs	299:302	MFCs	299:302	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	1	44	theme	fuel	287:290	arg1	cells					292:296	air-cathode microbial fuel cells	265:296	air-cathode microbial fuel cells (MFCs)	265:303	In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs).
25877400	3	45	theme	products	554:561	arg1	oxidation					503:511	the oxidation	499:511	the oxidation of several common microbial fermentation products (formate, lactate, and ethanol)	499:593	The activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (formate, lactate, and ethanol) was studied for MFC based on Klebsiella pneumoniae conditions.
25877400	6	46	theme	higher	1013:1018	arg1	crystallinity					1020:1032	its higher crystallinity	1009:1032	its higher crystallinity	1009:1032	The electrocatalytic experiments show that the Ni/β-Mo2C(G) gives the better bio- and electrocatalytic performance than Ni/β-Mo2C(S) due to its higher crystallinity and BET surface area.
29352886	6	0	theme	complementary	1503:1515	arg1	fertilizers					1517:1527	complementary fertilizers	1503:1527	complementary fertilizers	1503:1527	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	6	0	theme	complementary	1503:1515	arg1	drugs					1542:1546	synergistic drugs	1530:1546	synergistic drugs	1530:1546	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	4	1	dep	properties	846:855	arg1	basis					800:804	basis	800:804	basis	800:804	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	4	1	dep	properties	846:855	arg1	the					796:798	the	796:798	the	796:798	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	6	2	theme	molecular	1404:1412	arg1	level					1414:1418	the molecular level	1400:1418	the molecular level	1400:1418	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	4	3	theme	clip	1031:1034	arg1	"					1035:1035	imine clip"	1025:1035	"imine clip"	1024:1035	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	4	4	theme	geometrical	815:825	arg1	properties					846:855	their geometrical and conformational properties	809:855	their geometrical and conformational properties	809:855	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	5	5	theme	conjugated	1303:1312	arg1	aromatics					1314:1322	the conjugated aromatics	1299:1322	the conjugated aromatics	1299:1322	The exfoliated clay nanoparticles within the carbohydrate polymer enables either accelerating or slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics.
29352886	5	6	theme	imine	1245:1249	arg1	hydrolysis					1256:1265	the imine (CN) hydrolysis	1241:1265	the imine (CN) hydrolysis	1241:1265	The exfoliated clay nanoparticles within the carbohydrate polymer enables either accelerating or slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics.
29352886	4	7	theme	intramolecular	992:1005	arg1	CNHO-Ar					1007:1013	intramolecular CNHO-Ar	992:1013	intramolecular CNHO-Ar known as "imine clip"	992:1035	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	6	8	theme	tuning	1366:1371	arg1	interactions					1384:1395	fine tuning host-guest interactions	1361:1395	fine tuning host-guest interactions at the molecular level	1361:1418	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	6	9	dep	chemicals	1487:1495	arg1	fertilizers					1517:1527	complementary fertilizers	1503:1527	complementary fertilizers	1503:1527	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	6	9	dep	chemicals	1487:1495	arg1	drugs					1542:1546	synergistic drugs	1530:1546	synergistic drugs	1530:1546	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	1	10	theme	straightforward	310:324	arg1	concepts					326:333	straightforward concepts	310:333	straightforward concepts enabling to circumvent this bottleneck	310:372	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	3	11	from	aldehydes	751:759	arg1	assembly					692:699	a ternary assembly	682:699	a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles	682:790	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	4	12	from	groups	873:878	arg1	aromatics					895:903	the grafted aromatics	883:903	the grafted aromatics	883:903	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	1	13	dep	e.g.	186:189	arg1	drugs					192:196	drugs	192:196	e.g.: drugs	186:196	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	2	14	theme	bioplastics	611:621	arg1	films					531:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films	449:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics	449:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	2	14	theme	bioplastics	611:621	arg1	generation					555:564	a new generation	549:564	a new generation of eco-friendly, controlled-chemical release bioplastics	549:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	1	15	from	missing	378:384	arg1	literature					398:407	the open literature	389:407	the open literature	389:407	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	2	16	theme	release	603:609	arg1	bioplastics					611:621	eco-friendly, controlled-chemical release bioplastics	569:621	eco-friendly, controlled-chemical release bioplastics	569:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	4	17	theme	conformational	831:844	arg1	properties					846:855	their geometrical and conformational properties	809:855	their geometrical and conformational properties	809:855	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	6	18	theme	host-guest	1373:1382	arg1	interactions					1384:1395	fine tuning host-guest interactions	1361:1395	fine tuning host-guest interactions at the molecular level	1361:1418	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	1	19	theme	Temporal	122:129	arg1	release					131:137	Temporal release	122:137	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs)	122:197	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	3	20	from	biopolymer	730:739	arg1	assembly					692:699	a ternary assembly	682:699	a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles	682:790	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	2	21	theme	aldehyde-functionalized	449:471	arg1	films					531:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films	449:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics	449:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	2	21	theme	aldehyde-functionalized	449:471	arg1	generation					555:564	a new generation	549:564	a new generation of eco-friendly, controlled-chemical release bioplastics	549:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	3	22	link	derived	715:721	arg1	biopolymer					730:739	biowaste derived chitin biopolymer	706:739	biowaste derived chitin biopolymer	706:739	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	4	23	theme	intermolecular	1101:1114	arg1	Si-OHO-Ar					1116:1124	intermolecular Si-OHO-Ar	1101:1124	intermolecular Si-OHO-Ar	1101:1124	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	3	24	theme	dynamically-assembled	630:650	arg1	nanomaterials					652:664	These dynamically-assembled nanomaterials	624:664	These dynamically-assembled nanomaterials	624:664	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	6	25	theme	different	1477:1485	arg1	chemicals					1487:1495	different chemicals	1477:1495	different chemicals (e.g. complementary fertilizers, synergistic drugs)	1477:1547	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	6	26	theme	fine	1361:1364	arg1	interactions					1384:1395	fine tuning host-guest interactions	1361:1395	fine tuning host-guest interactions at the molecular level	1361:1418	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	4	27	theme	clay	1083:1086	arg1	surface					1088:1094	the clay surface	1079:1094	the clay surface	1079:1094	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	4	28	theme	oxygenated	862:871	arg1	groups					873:878	the oxygenated groups	858:878	the oxygenated groups on the grafted aromatics	858:903	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	4	29	dep	with	929:932	arg1	either					934:939	either	934:939	either	934:939	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	0	30	theme	Aldehyde-functionalized	0:22	arg1	chitosan-montmorillonite					24:47	Aldehyde-functionalized chitosan-montmorillonite	0:47	Aldehyde-functionalized chitosan-montmorillonite	0:47	Aldehyde-functionalized chitosan-montmorillonite films as dynamically-assembled, switchable-chemical release bioplastics.
29352886	5	31	theme	CN	1252:1253	arg1	hydrolysis					1256:1265	the imine (CN) hydrolysis	1241:1265	the imine (CN) hydrolysis	1241:1265	The exfoliated clay nanoparticles within the carbohydrate polymer enables either accelerating or slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics.
29352886	1	32	theme	synergistic	142:152	arg1	chemicals					175:183	synergistic and/or complementary chemicals	142:183	synergistic and/or complementary chemicals (e.g.: drugs)	142:197	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	4	33	theme	grafted	887:893	arg1	aromatics					895:903	the grafted aromatics	883:903	the grafted aromatics	883:903	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	3	34	dep	derived	715:721	arg1	biowaste					706:713	biowaste	706:713	biowaste	706:713	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	2	35	theme	hybrid	524:529	arg1	films					531:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films	449:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics	449:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	2	35	theme	hybrid	524:529	arg1	generation					555:564	a new generation	549:564	a new generation of eco-friendly, controlled-chemical release bioplastics	549:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	2	36	theme	flexible	490:497	arg1	films					531:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films	449:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics	449:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	2	36	theme	flexible	490:497	arg1	generation					555:564	a new generation	549:564	a new generation of eco-friendly, controlled-chemical release bioplastics	549:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	2	37	theme	chitosan-montmorillonite	499:522	arg1	films					531:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films	449:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics	449:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	2	37	theme	chitosan-montmorillonite	499:522	arg1	generation					555:564	a new generation	549:564	a new generation of eco-friendly, controlled-chemical release bioplastics	549:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	6	38	theme	sequential	1562:1571	arg1	release					1573:1579	their sequential release	1556:1579	their sequential release	1556:1579	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	1	39	dep	chemicals	175:183	arg1	e.g.					186:189	e.g.	186:189	e.g.: drugs	186:196	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	3	40	theme	layered	765:771	arg1	nanoparticles					778:790	layered clay nanoparticles	765:790	layered clay nanoparticles	765:790	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	1	41	theme	complementary	161:173	arg1	chemicals					175:183	synergistic and/or complementary chemicals	142:183	synergistic and/or complementary chemicals (e.g.: drugs)	142:197	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	2	42	theme	new	551:553	arg1	films					531:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films	449:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics	449:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	2	42	theme	new	551:553	arg1	generation					555:564	a new generation	549:564	a new generation of eco-friendly, controlled-chemical release bioplastics	549:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	6	43	theme	subtle	1452:1457	arg1	discrimination					1459:1472	subtle discrimination	1452:1472	subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs)	1452:1547	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	3	44	theme	clay	773:776	arg1	nanoparticles					778:790	layered clay nanoparticles	765:790	layered clay nanoparticles	765:790	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	4	45	theme	Schiff	950:955	arg1	base					945:948	the base Schiff	941:955	the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip")	941:1036	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	2	46	theme	transparent	474:484	arg1	films					531:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films	449:535	aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics	449:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	2	46	theme	transparent	474:484	arg1	generation					555:564	a new generation	549:564	a new generation of eco-friendly, controlled-chemical release bioplastics	549:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	0	47	theme	dynamically-assembled	58:78	arg1	bioplastics					109:119	dynamically-assembled, switchable-chemical release bioplastics	58:119	dynamically-assembled, switchable-chemical release bioplastics	58:119	Aldehyde-functionalized chitosan-montmorillonite films as dynamically-assembled, switchable-chemical release bioplastics.
29352886	5	48	theme	aromatics	1314:1322	arg1	interaction					1284:1294	the interaction	1280:1294	the interaction of the conjugated aromatics	1280:1322	The exfoliated clay nanoparticles within the carbohydrate polymer enables either accelerating or slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics.
29352886	3	49	theme	derived	715:721	arg1	biopolymer					730:739	biowaste derived chitin biopolymer	706:739	biowaste derived chitin biopolymer	706:739	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	1	50	theme	intertwined	266:276	arg1	delivery					286:293	their frequently intertwined kinetic delivery	249:293	their frequently intertwined kinetic delivery	249:293	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	0	51	theme	release	101:107	arg1	bioplastics					109:119	dynamically-assembled, switchable-chemical release bioplastics	58:119	dynamically-assembled, switchable-chemical release bioplastics	58:119	Aldehyde-functionalized chitosan-montmorillonite films as dynamically-assembled, switchable-chemical release bioplastics.
29352886	6	52	from	level	1414:1418	arg1	interactions					1384:1395	fine tuning host-guest interactions	1361:1395	fine tuning host-guest interactions at the molecular level	1361:1418	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	4	53	theme	imine	1025:1029	arg1	"					1035:1035	imine clip"	1025:1035	"imine clip"	1024:1035	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
29352886	5	54	theme	exfoliated	1132:1141	arg1	clay					1143:1146	The exfoliated clay	1128:1146	The exfoliated clay	1128:1146	The exfoliated clay nanoparticles within the carbohydrate polymer enables either accelerating or slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics.
29352886	1	55	theme	chemicals	175:183	arg1	release					131:137	Temporal release	122:137	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs)	122:197	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	1	56	from	literature	398:407	arg1	missing					378:384	missing	378:384	missing	378:384	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	0	57	dep	dynamically-assembled	58:78	arg1	switchable-chemical					81:99	switchable-chemical	81:99	switchable-chemical	81:99	Aldehyde-functionalized chitosan-montmorillonite films as dynamically-assembled, switchable-chemical release bioplastics.
29352886	6	58	dep	fertilizers	1517:1527	arg1	e.g.					1498:1501	e.g.	1498:1501	e.g.	1498:1501	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	6	59	theme	driving	1343:1349	arg1	force					1351:1355	the driving force	1339:1355	the driving force for fine tuning host-guest interactions at the molecular level	1339:1418	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	1	60	theme	kinetic	278:284	arg1	delivery					286:293	their frequently intertwined kinetic delivery	249:293	their frequently intertwined kinetic delivery	249:293	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	2	61	dep	eco-friendly	569:580	arg1	controlled-chemical					583:601	controlled-chemical	583:601	controlled-chemical	583:601	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	3	62	theme	aromatic	742:749	arg1	aldehydes					751:759	aromatic aldehydes	742:759	aromatic aldehydes	742:759	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	6	63	theme	chemicals	1487:1495	arg1	discrimination					1459:1472	subtle discrimination	1452:1472	subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs)	1452:1547	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	3	64	from	nanoparticles	778:790	arg1	assembly					692:699	a ternary assembly	682:699	a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles	682:790	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	5	65	theme	carbohydrate	1173:1184	arg1	polymer					1186:1192	the carbohydrate polymer	1169:1192	the carbohydrate polymer enables either accelerating or slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics	1169:1322	The exfoliated clay nanoparticles within the carbohydrate polymer enables either accelerating or slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics.
29352886	3	66	theme	chitin	723:728	arg1	biopolymer					730:739	biowaste derived chitin biopolymer	706:739	biowaste derived chitin biopolymer	706:739	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	6	67	theme	synergistic	1530:1540	arg1	fertilizers					1517:1527	complementary fertilizers	1503:1527	complementary fertilizers	1503:1527	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	6	67	theme	synergistic	1530:1540	arg1	drugs					1542:1546	synergistic drugs	1530:1546	synergistic drugs	1530:1546	This provides the driving force for fine tuning host-guest interactions at the molecular level and constitutes an entry toward subtle discrimination of different chemicals (e.g. complementary fertilizers, synergistic drugs) during their sequential release.
29352886	5	68	dep	polymer	1186:1192	arg1	enables					1194:1200	enables	1194:1200	enables either accelerating or slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics	1194:1322	The exfoliated clay nanoparticles within the carbohydrate polymer enables either accelerating or slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics.
29352886	2	69	theme	eco-friendly	569:580	arg1	bioplastics					611:621	eco-friendly, controlled-chemical release bioplastics	569:621	eco-friendly, controlled-chemical release bioplastics	569:621	In this framework, we report herein on aldehyde-functionalized, transparent and flexible chitosan-montmorillonite hybrid films that act as a new generation of eco-friendly, controlled-chemical release bioplastics.
29352886	1	70	theme	open	393:396	arg1	literature					398:407	the open literature	389:407	the open literature	389:407	Temporal release of synergistic and/or complementary chemicals (e.g.: drugs) is recognized as extremely challenging because of their frequently intertwined kinetic delivery and presently, straightforward concepts enabling to circumvent this bottleneck are missing in the open literature.
29352886	5	71	dep	enables	1194:1200	arg1	slowing					1225:1231	slowing	1225:1231	slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics	1225:1322	The exfoliated clay nanoparticles within the carbohydrate polymer enables either accelerating or slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics.
29352886	5	71	dep	enables	1194:1200	arg1	accelerating					1209:1220	accelerating	1209:1220	accelerating	1209:1220	The exfoliated clay nanoparticles within the carbohydrate polymer enables either accelerating or slowing down of the imine (CN) hydrolysis depending on the interaction of the conjugated aromatics.
29352886	3	72	theme	ternary	684:690	arg1	assembly					692:699	a ternary assembly	682:699	a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles	682:790	These dynamically-assembled nanomaterials are designed by a ternary assembly from biowaste derived chitin biopolymer, aromatic aldehydes and layered clay nanoparticles.
29352886	4	73	theme	hydroxyl	1050:1057	arg1	groups					1059:1064	the hydroxyl groups	1046:1064	the hydroxyl groups belonging to the clay surface	1046:1094	On the basis of their geometrical and conformational properties, the oxygenated groups on the grafted aromatics interact preferentially with either the base Schiff belonging to the carbohydrate (via intramolecular CNHO-Ar known as "imine clip") or with the hydroxyl groups belonging to the clay surface (via intermolecular Si-OHO-Ar).
27902294	3	0	theme	purple-pigmented	287:302	arg1	filaments					304:312	purple-pigmented filaments	287:312	purple-pigmented filaments	287:312	Cells of isolate OB1T were Gram-stain-positive, non-motile, pink- to purple-pigmented filaments.
27902294	2	1	theme	aerobic	102:108	arg1	actinobacterium					130:144	An aerobic, mildly acidophilic actinobacterium	99:144	An aerobic, mildly acidophilic actinobacterium	99:144	An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada.
27902294	11	2	theme	gene	1051:1054	arg1	analysis					1065:1072	comparative 16S rRNA gene sequence analysis	1030:1072	comparative 16S rRNA gene sequence analysis	1030:1072	On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales.
27902294	11	3	theme	sequence	1056:1063	arg1	analysis					1065:1072	comparative 16S rRNA gene sequence analysis	1030:1072	comparative 16S rRNA gene sequence analysis	1030:1072	On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales.
27902294	11	4	theme	rRNA	1046:1049	arg1	analysis					1065:1072	comparative 16S rRNA gene sequence analysis	1030:1072	comparative 16S rRNA gene sequence analysis	1030:1072	On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales.
27902294	2	5	from	bog	179:181	arg1	Canada					210:215	Canada	210:215	Canada	210:215	An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada.
27902294	5	6	theme	major	519:523	arg1	phosphohexose					541:553	phosphohexose	541:553	phosphohexose	541:553	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	5	6	theme	major	519:523	arg1	lipid					531:535	the major polar lipid	515:535	the major polar lipid	515:535	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	13	7	theme	16S	1264:1266	arg1	rRNA					1268:1271	16S rRNA	1264:1271	the 16S rRNA gene sequence identity to this bacterium	1260:1312	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	13	8	theme	several	1346:1352	arg1	differences					1369:1379	several chemotaxonomic differences	1346:1379	several chemotaxonomic differences from this species	1346:1397	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	13	9	dep	low	1318:1320	arg1	identity					1287:1294	the 16S rRNA gene sequence identity	1260:1294	the 16S rRNA gene sequence identity to this bacterium	1260:1312	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	13	9	dep	low	1318:1320	arg1	low					1318:1320	low	1318:1320	low	1318:1320	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	13	9	dep	low	1318:1320	arg1	%					1328:1328	92.8 %	1323:1328	92.8 %	1323:1328	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	14	10	theme	novel	1423:1427	arg1	genus					1429:1433	a novel genus	1421:1433	a novel genus	1421:1433	We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp.
27902294	14	10	theme	novel	1423:1427	arg1	nov.					1480:1483	Actinocrinis puniceicyclus gen. nov.	1448:1483	Actinocrinis puniceicyclus gen. nov.	1448:1483	We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp.
27902294	5	11	theme	polar	525:529	arg1	phosphohexose					541:553	phosphohexose	541:553	phosphohexose	541:553	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	5	11	theme	polar	525:529	arg1	lipid					531:535	the major polar lipid	515:535	the major polar lipid	515:535	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	13	12	theme	chemotaxonomic	1354:1367	arg1	differences					1369:1379	several chemotaxonomic differences	1346:1379	several chemotaxonomic differences from this species	1346:1397	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	10	13	theme	atmospheric	992:1002	arg1	nitrogen					1004:1011	atmospheric nitrogen	992:1011	atmospheric nitrogen	992:1011	The cells did not fix atmospheric nitrogen.
27902294	11	14	theme	order	1169:1173	arg1	Actinomycetales					1175:1189	the order Actinomycetales	1165:1189	the order Actinomycetales	1165:1189	On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales.
27902294	15	15	dep	OB1T	1508:1511	arg1	strain					1554:1559	the type strain	1545:1559	the type strain	1545:1559	nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain.
27902294	8	16	theme	complex	691:697	arg1	acids					757:761	casamino acids	748:761	casamino acids	748:761	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	16	theme	complex	691:697	arg1	broth					779:783	tryptic soy broth	767:783	tryptic soy broth	767:783	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	16	theme	complex	691:697	arg1	media					699:703	complex media	691:703	complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth	691:783	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	16	theme	complex	691:697	arg1	extract					721:727	yeast extract	715:727	yeast extract	715:727	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	16	theme	complex	691:697	arg1	peptone					739:745	proteose peptone	730:745	proteose peptone	730:745	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	13	17	theme	rRNA	1268:1271	arg1	%					1328:1328	92.8 %	1323:1328	92.8 %	1323:1328	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	13	17	theme	rRNA	1268:1271	arg1	low					1318:1320	low	1318:1320	low	1318:1320	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	13	17	theme	rRNA	1268:1271	arg1	identity					1287:1294	the 16S rRNA gene sequence identity	1260:1294	the 16S rRNA gene sequence identity to this bacterium	1260:1312	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	11	18	theme	Actinomycetales	1175:1189	arg1	Catenulisporineae					1144:1160	the suborder Catenulisporineae	1131:1160	the suborder Catenulisporineae of the order Actinomycetales	1131:1189	On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales.
27902294	3	19	theme	isolate	227:233	arg1	OB1T					235:238	isolate OB1T	227:238	isolate OB1T	227:238	Cells of isolate OB1T were Gram-stain-positive, non-motile, pink- to purple-pigmented filaments.
27902294	11	20	theme	family	1104:1109	arg1	Actinospicaceae					1111:1125	the family Actinospicaceae	1100:1125	the family Actinospicaceae	1100:1125	On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales.
27902294	6	21	theme	major	560:564	arg1	menaquinone-11					578:591	menaquinone-11	578:591	menaquinone-11	578:591	The major quinone was menaquinone-11 (MK-11), and the peptidoglycan type was A1γ.
27902294	6	21	theme	major	560:564	arg1	quinone					566:572	The major quinone	556:572	The major quinone	556:572	The major quinone was menaquinone-11 (MK-11), and the peptidoglycan type was A1γ.
27902294	13	22	theme	gene	1273:1276	arg1	%					1328:1328	92.8 %	1323:1328	92.8 %	1323:1328	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	13	22	theme	gene	1273:1276	arg1	low					1318:1320	low	1318:1320	low	1318:1320	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	13	22	theme	gene	1273:1276	arg1	identity					1287:1294	the 16S rRNA gene sequence identity	1260:1294	the 16S rRNA gene sequence identity to this bacterium	1260:1312	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	0	23	theme	puniceicyclus	13:25	arg1	nov.					32:35	Actinocrinis puniceicyclus gen. nov.	0:35	Actinocrinis puniceicyclus gen. nov.	0:35	Actinocrinis puniceicyclus gen. nov., sp.
27902294	12	24	theme	related	1209:1215	arg1	species					1217:1223	The most closely related species	1192:1223	The most closely related species	1192:1223	The most closely related species was Actinospica robiniae.
27902294	12	24	theme	related	1209:1215	arg1	robiniae					1241:1248	Actinospica robiniae	1229:1248	Actinospica robiniae	1229:1248	The most closely related species was Actinospica robiniae.
27902294	13	25	theme	sequence	1278:1285	arg1	%					1328:1328	92.8 %	1323:1328	92.8 %	1323:1328	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	13	25	theme	sequence	1278:1285	arg1	low					1318:1320	low	1318:1320	low	1318:1320	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	13	25	theme	sequence	1278:1285	arg1	identity					1287:1294	the 16S rRNA gene sequence identity	1260:1294	the 16S rRNA gene sequence identity to this bacterium	1260:1312	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
27902294	0	26	theme	Actinocrinis	0:11	arg1	nov.					32:35	Actinocrinis puniceicyclus gen. nov.	0:35	Actinocrinis puniceicyclus gen. nov.	0:35	Actinocrinis puniceicyclus gen. nov., sp.
27902294	7	27	theme	G+C	646:648	arg1	%					667:667	70.2 %	662:667	70.2 %	662:667	The DNA G+C content was 70.2 %.
27902294	7	27	theme	G+C	646:648	arg1	content					650:656	The DNA G+C content	638:656	The DNA G+C content	638:656	The DNA G+C content was 70.2 %.
27902294	8	28	from	growth	681:686	arg1	acids					757:761	casamino acids	748:761	casamino acids	748:761	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	28	from	growth	681:686	arg1	broth					779:783	tryptic soy broth	767:783	tryptic soy broth	767:783	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	28	from	growth	681:686	arg1	media					699:703	complex media	691:703	complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth	691:783	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	28	from	growth	681:686	arg1	extract					721:727	yeast extract	715:727	yeast extract	715:727	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	28	from	growth	681:686	arg1	peptone					739:745	proteose peptone	730:745	proteose peptone	730:745	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	5	29	theme	cellular	421:428	arg1	acids					436:440	The major cellular fatty acids	411:440	The major cellular fatty acids	411:440	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	5	29	theme	cellular	421:428	arg1	i-C16 					447:452	i-C16 	447:452	i-C16 	447:452	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	15	30	dep	nov.	1490:1493	arg1	with					1496:1499	with	1496:1499	with	1496:1499	nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain.
27902294	1	31	theme	acidic	84:89	arg1	spring					91:96	an acidic spring	81:96	an acidic spring	81:96	nov., an actinobacterium isolated from an acidic spring.
27902294	5	32	theme	fatty	430:434	arg1	acids					436:440	The major cellular fatty acids	411:440	The major cellular fatty acids	411:440	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	5	32	theme	fatty	430:434	arg1	i-C16 					447:452	i-C16 	447:452	i-C16 	447:452	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	3	33	dep	Gram-stain-positive	245:263	arg1	pink-					278:282	pink-	278:282	pink-	278:282	Cells of isolate OB1T were Gram-stain-positive, non-motile, pink- to purple-pigmented filaments.
27902294	3	33	dep	Gram-stain-positive	245:263	arg1	non-motile					266:275	non-motile	266:275	non-motile	266:275	Cells of isolate OB1T were Gram-stain-positive, non-motile, pink- to purple-pigmented filaments.
27902294	0	34	theme	gen.	27:30	arg1	nov.					32:35	Actinocrinis puniceicyclus gen. nov.	0:35	Actinocrinis puniceicyclus gen. nov.	0:35	Actinocrinis puniceicyclus gen. nov., sp.
27902294	1	35	attach	isolated	67:74	arg1	spring					91:96	an acidic spring	81:96	an acidic spring	81:96	nov., an actinobacterium isolated from an acidic spring.
27902294	1	35	attach	isolated	67:74	arg2	actinobacterium					51:65	an actinobacterium	48:65	an actinobacterium isolated from an acidic spring	48:96	nov., an actinobacterium isolated from an acidic spring.
27902294	2	36	theme	Ochre	168:172	arg1	bog					179:181	the Ochre Beds bog	164:181	the Ochre Beds bog in Kootenay National Park, Canada	164:215	An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada.
27902294	15	37	theme	=DSM	1514:1517	arg1	BAA-2771T					1531:1539	=DSM 45618T=ATCC BAA-2771T	1514:1539	=DSM 45618T=ATCC BAA-2771T	1514:1539	nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain.
27902294	15	37	theme	=DSM	1514:1517	arg1	OB1T					1508:1511	strain OB1T	1501:1511	strain OB1T (=DSM 45618T=ATCC BAA-2771T)	1501:1540	nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain.
27902294	8	38	dep	mono-	804:808	arg1	xylose					861:866	xylose	861:866	xylose	861:866	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	38	dep	mono-	804:808	arg1	galactose					847:855	galactose	847:855	galactose	847:855	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	38	dep	mono-	804:808	arg1	sucrose					838:844	sucrose	838:844	sucrose	838:844	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	38	dep	mono-	804:808	arg1	glucose					829:835	glucose	829:835	glucose	829:835	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	11	39	dep	analysis	1065:1072	arg1	basis					1021:1025	basis	1021:1025	basis	1021:1025	On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales.
27902294	11	39	dep	analysis	1065:1072	arg1	the					1017:1019	the	1017:1019	the	1017:1019	On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales.
27902294	7	40	theme	DNA	642:644	arg1	%					667:667	70.2 %	662:667	70.2 %	662:667	The DNA G+C content was 70.2 %.
27902294	7	40	theme	DNA	642:644	arg1	content					650:656	The DNA G+C content	638:656	The DNA G+C content	638:656	The DNA G+C content was 70.2 %.
27902294	3	41	theme	OB1T	235:238	arg1	Cells					218:222	Cells	218:222	Cells of isolate OB1T	218:238	Cells of isolate OB1T were Gram-stain-positive, non-motile, pink- to purple-pigmented filaments.
27902294	14	42	theme	gen.	1475:1478	arg1	genus					1429:1433	a novel genus	1421:1433	a novel genus	1421:1433	We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp.
27902294	14	42	theme	gen.	1475:1478	arg1	sp					1486:1487	sp	1486:1487	sp	1486:1487	We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp.
27902294	14	42	theme	gen.	1475:1478	arg1	nov.					1480:1483	Actinocrinis puniceicyclus gen. nov.	1448:1483	Actinocrinis puniceicyclus gen. nov.	1448:1483	We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp.
27902294	8	43	theme	proteose	730:737	arg1	peptone					739:745	proteose peptone	730:745	proteose peptone	730:745	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	2	44	attach	isolated	150:157	arg1	bog					179:181	the Ochre Beds bog	164:181	the Ochre Beds bog in Kootenay National Park, Canada	164:215	An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada.
27902294	2	44	attach	isolated	150:157	arg2	actinobacterium					130:144	An aerobic, mildly acidophilic actinobacterium	99:144	An aerobic, mildly acidophilic actinobacterium	99:144	An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada.
27902294	11	45	theme	suborder	1135:1142	arg1	Catenulisporineae					1144:1160	the suborder Catenulisporineae	1131:1160	the suborder Catenulisporineae of the order Actinomycetales	1131:1189	On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales.
27902294	14	46	theme	puniceicyclus	1461:1473	arg1	genus					1429:1433	a novel genus	1421:1433	a novel genus	1421:1433	We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp.
27902294	14	46	theme	puniceicyclus	1461:1473	arg1	sp					1486:1487	sp	1486:1487	sp	1486:1487	We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp.
27902294	14	46	theme	puniceicyclus	1461:1473	arg1	nov.					1480:1483	Actinocrinis puniceicyclus gen. nov.	1448:1483	Actinocrinis puniceicyclus gen. nov.	1448:1483	We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp.
27902294	6	47	theme	peptidoglycan	610:622	arg1	A1γ					633:635	A1γ	633:635	A1γ	633:635	The major quinone was menaquinone-11 (MK-11), and the peptidoglycan type was A1γ.
27902294	6	47	theme	peptidoglycan	610:622	arg1	type					624:627	the peptidoglycan type	606:627	the peptidoglycan type	606:627	The major quinone was menaquinone-11 (MK-11), and the peptidoglycan type was A1γ.
27902294	9	48	theme	Anaerobic	935:943	arg1	growth					945:950	Anaerobic growth	935:950	Anaerobic growth	935:950	Anaerobic growth was not observed.
27902294	14	49	theme	Actinocrinis	1448:1459	arg1	genus					1429:1433	a novel genus	1421:1433	a novel genus	1421:1433	We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp.
27902294	14	49	theme	Actinocrinis	1448:1459	arg1	sp					1486:1487	sp	1486:1487	sp	1486:1487	We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp.
27902294	14	49	theme	Actinocrinis	1448:1459	arg1	nov.					1480:1483	Actinocrinis puniceicyclus gen. nov.	1448:1483	Actinocrinis puniceicyclus gen. nov.	1448:1483	We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp.
27902294	0	50	dep	sp	38:39	arg1	nov.					32:35	Actinocrinis puniceicyclus gen. nov.	0:35	Actinocrinis puniceicyclus gen. nov.	0:35	Actinocrinis puniceicyclus gen. nov., sp.
27902294	4	51	theme	pH	319:320	arg1	range					322:326	The pH range	315:326	The pH range for growth	315:337	The pH range for growth was pH 3.5-6.5 (optimum pH 5.5), and the temperature range was 13-30°C.
27902294	4	51	theme	pH	319:320	arg1	pH 3.5-6.5					343:352	pH 3.5-6.5	343:352	pH 3.5-6.5	343:352	The pH range for growth was pH 3.5-6.5 (optimum pH 5.5), and the temperature range was 13-30°C.
27902294	1	52	dep	actinobacterium	51:65	arg1	nov.					42:45	nov.	42:45	nov.	42:45	nov., an actinobacterium isolated from an acidic spring.
27902294	8	53	dep	polysaccharides	873:887	arg1	pectin					906:911	pectin	906:911	pectin	906:911	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	53	dep	polysaccharides	873:887	arg1	polysaccharides					873:887	polysaccharides	873:887	polysaccharides (starch, gellan, pectin, xylan and alginate)	873:932	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	53	dep	polysaccharides	873:887	arg1	starch					890:895	starch	890:895	starch	890:895	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	53	dep	polysaccharides	873:887	arg1	xylan					914:918	xylan	914:918	xylan	914:918	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	53	dep	polysaccharides	873:887	arg1	alginate					924:931	alginate	924:931	alginate	924:931	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	53	dep	polysaccharides	873:887	arg1	gellan					898:903	gellan	898:903	gellan	898:903	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	11	54	theme	comparative	1030:1040	arg1	analysis					1065:1072	comparative 16S rRNA gene sequence analysis	1030:1072	comparative 16S rRNA gene sequence analysis	1030:1072	On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales.
27902294	4	55	theme	temperature	380:390	arg1	range					392:396	the temperature range	376:396	the temperature range	376:396	The pH range for growth was pH 3.5-6.5 (optimum pH 5.5), and the temperature range was 13-30°C.
27902294	5	56	dep	i-C16 	447:452	arg1	 0					454:455	 0	454:455	 0	454:455	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	5	56	dep	i-C16 	447:452	arg1	%					507:507	14.3 %	502:507	14.3 %	502:507	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	5	56	dep	i-C16 	447:452	arg1	 0					474:475	 0	474:475	 0	474:475	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	5	56	dep	i-C16 	447:452	arg1	ai-C15 					490:496	ai-C15 	490:496	ai-C15 	490:496	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	5	56	dep	i-C16 	447:452	arg1	 0					498:499	 0	498:499	 0	498:499	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	5	56	dep	i-C16 	447:452	arg1	%					463:463	28.5 %	458:463	28.5 %	458:463	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	5	56	dep	i-C16 	447:452	arg1	%					483:483	14.6 %	478:483	14.6 %	478:483	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	11	57	theme	16S	1042:1044	arg1	analysis					1065:1072	comparative 16S rRNA gene sequence analysis	1030:1072	comparative 16S rRNA gene sequence analysis	1030:1072	On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales.
27902294	15	58	theme	strain	1501:1506	arg1	BAA-2771T					1531:1539	=DSM 45618T=ATCC BAA-2771T	1514:1539	=DSM 45618T=ATCC BAA-2771T	1514:1539	nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain.
27902294	15	58	theme	strain	1501:1506	arg1	OB1T					1508:1511	strain OB1T	1501:1511	strain OB1T (=DSM 45618T=ATCC BAA-2771T)	1501:1540	nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain.
27902294	2	59	dep	aerobic	102:108	arg1	acidophilic					118:128	acidophilic	118:128	acidophilic	118:128	An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada.
27902294	15	60	theme	45618T=ATCC	1519:1529	arg1	BAA-2771T					1531:1539	=DSM 45618T=ATCC BAA-2771T	1514:1539	=DSM 45618T=ATCC BAA-2771T	1514:1539	nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain.
27902294	15	60	theme	45618T=ATCC	1519:1529	arg1	OB1T					1508:1511	strain OB1T	1501:1511	strain OB1T (=DSM 45618T=ATCC BAA-2771T)	1501:1540	nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain.
27902294	8	61	theme	casamino	748:755	arg1	acids					757:761	casamino acids	748:761	casamino acids	748:761	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	62	theme	soy	775:777	arg1	broth					779:783	tryptic soy broth	767:783	tryptic soy broth	767:783	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	8	63	theme	yeast	715:719	arg1	extract					721:727	yeast extract	715:727	yeast extract	715:727	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	2	64	theme	Beds	174:177	arg1	bog					179:181	the Ochre Beds bog	164:181	the Ochre Beds bog in Kootenay National Park, Canada	164:215	An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada.
27902294	8	65	theme	tryptic	767:773	arg1	broth					779:783	tryptic soy broth	767:783	tryptic soy broth	767:783	Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate).
27902294	5	66	theme	major	415:419	arg1	acids					436:440	The major cellular fatty acids	411:440	The major cellular fatty acids	411:440	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	5	66	theme	major	415:419	arg1	i-C16 					447:452	i-C16 	447:452	i-C16 	447:452	The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose.
27902294	15	67	theme	type	1549:1552	arg1	strain					1554:1559	the type strain	1545:1559	the type strain	1545:1559	nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain.
27902294	12	68	theme	Actinospica	1229:1239	arg1	species					1217:1223	The most closely related species	1192:1223	The most closely related species	1192:1223	The most closely related species was Actinospica robiniae.
27902294	12	68	theme	Actinospica	1229:1239	arg1	robiniae					1241:1248	Actinospica robiniae	1229:1248	Actinospica robiniae	1229:1248	The most closely related species was Actinospica robiniae.
27902294	13	69	from	species	1391:1397	arg1	differences					1369:1379	several chemotaxonomic differences	1346:1379	several chemotaxonomic differences from this species	1346:1397	However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species.
29210453	1	0	dep	techniques	419:428	arg1	vanillin+ultrasound					444:462	vanillin+ultrasound	444:462	vanillin+ultrasound	444:462	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	1	0	dep	techniques	419:428	arg1	geraniol					431:438	geraniol	431:438	geraniol	431:438	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	1	0	dep	techniques	419:428	arg1	techniques					419:428	nonthermal techniques	408:428	nonthermal techniques (geraniol and vanillin+ultrasound)	408:463	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	13	1	theme	rapid	2080:2084	arg1	monitoring					2086:2095	the rapid monitoring	2076:2095	the rapid monitoring of juices' authenticity and/or deterioration	2076:2140	The robust 21 PLS models defined in this work provided reliable tools for the rapid monitoring of juices' authenticity and/or deterioration.
29210453	2	2	dep	region	534:539	arg1	to					522:523	to	522:523	to	522:523	The main spectral differences were observed in the 1200 to 900 cm-1 region.
29210453	10	3	theme	unknown	1636:1642	arg1	samples					1644:1650	unknown samples	1636:1650	unknown samples	1636:1650	The mean of predicted values fitted nicely those obtained by HPLC (correlation and R2 > 0.97), thus supporting the use of the PLS models to monitor the quality of strawberry juices in unknown samples.
29210453	4	4	theme	high-performance	812:827	arg1	chromatography					836:849	high-performance liquid chromatography	812:849	high-performance liquid chromatography (HPLC)	812:856	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	4	4	theme	high-performance	812:827	arg1	HPLC					852:855	HPLC	852:855	HPLC	852:855	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	7	5	theme	square	1133:1138	arg1	PLS					1148:1150	PLS	1148:1150	PLS	1148:1150	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	7	5	theme	square	1133:1138	arg1	models					1140:1145	different partial least square models	1109:1145	different partial least square models (PLS)	1109:1151	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	14	6	theme	pure	2290:2293	arg1	sugars					2295:2300	pure sugars	2290:2300	pure sugars as standards	2290:2313	In this regard, FTIR associated to multivariate analysis enabled the determination of different sugars in a single measurement without the need of pure sugars as standards.
29210453	12	7	theme	transferable	1920:1931	arg1	method					1901:1906	a method	1899:1906	a method potentially transferable to the food industry	1899:1952	PRACTICAL APPLICATION FTIR spectroscopy provided a method potentially transferable to the food industry when associated with the multivariate analysis.
29210453	0	8	theme	Fructans	120:127	arg1	Determination					92:104	the Simultaneous Determination	75:104	the Simultaneous Determination of Sugars and Fructans in Strawberry	75:141	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry Juices During Storage.
29210453	12	9	theme	FTIR	1872:1875	arg1	spectroscopy					1877:1888	PRACTICAL APPLICATION FTIR spectroscopy	1850:1888	PRACTICAL APPLICATION FTIR spectroscopy	1850:1888	PRACTICAL APPLICATION FTIR spectroscopy provided a method potentially transferable to the food industry when associated with the multivariate analysis.
29210453	3	10	theme	typical	584:590	arg1	bands					592:596	the typical bands	580:596	the typical bands at 1134, 1034, and 935 cm-1	580:624	The presence of FOS was identified by the typical bands at 1134, 1034, and 935 cm-1 .
29210453	1	11	theme	spectroscopy	213:224	arg1	method					239:244	mid-infrared spectroscopy (FTIR)-based method	200:244	mid-infrared spectroscopy (FTIR)-based method	200:244	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	4	12	theme	sucrose	670:676	arg1	decrease					658:665	a significant decrease	644:665	a significant decrease of sucrose	644:676	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	4	12	theme	sucrose	670:676	arg1	concomitant					682:692	concomitant	682:692	concomitant	682:692	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	10	13	theme	R2	1535:1536	arg1	> 0.97					1538:1543	R2 > 0.97	1535:1543	R2 > 0.97	1535:1543	The mean of predicted values fitted nicely those obtained by HPLC (correlation and R2 > 0.97), thus supporting the use of the PLS models to monitor the quality of strawberry juices in unknown samples.
29210453	11	14	theme	analytical	1709:1718	arg1	tool					1720:1723	an adequate analytical tool	1697:1723	an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration	1697:1847	In conclusion, FTIR spectroscopy appears as an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration.
29210453	9	15	theme	independent	1426:1436	arg1	sets					1438:1441	independent sets	1426:1441	independent sets of data	1426:1449	The models were validated with independent sets of data.
29210453	9	15	theme	independent	1426:1436	arg1	data					1446:1449	data	1446:1449	data	1446:1449	The models were validated with independent sets of data.
29210453	15	16	theme	experimental	2321:2332	arg1	simplicity					2334:2343	This experimental simplicity	2316:2343	This experimental simplicity	2316:2343	This experimental simplicity supports the use of FTIR at the production line, and also contributes to save time in determining carbohydrates' composition and stability, in an environmentally friendly way.
29210453	0	17	from	Determination	92:104	arg1	Strawberry					132:141	Strawberry	132:141	Strawberry	132:141	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry Juices During Storage.
29210453	0	18	theme	Analysis	62:69	arg1	Approach					11:18	A Combined Approach	0:18	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry	0:141	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry Juices During Storage.
29210453	13	19	theme	robust	2006:2011	arg1	models					2020:2025	The robust 21 PLS models	2002:2025	The robust 21 PLS models defined in this work	2002:2046	The robust 21 PLS models defined in this work provided reliable tools for the rapid monitoring of juices' authenticity and/or deterioration.
29210453	15	20	theme	production	2377:2386	arg1	line					2388:2391	the production line	2373:2391	the production line	2373:2391	This experimental simplicity supports the use of FTIR at the production line, and also contributes to save time in determining carbohydrates' composition and stability, in an environmentally friendly way.
29210453	11	21	theme	FTIR	1668:1671	arg1	spectroscopy					1673:1684	FTIR spectroscopy	1668:1684	FTIR spectroscopy	1668:1684	In conclusion, FTIR spectroscopy appears as an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration.
29210453	13	22	theme	PLS	2016:2018	arg1	models					2020:2025	The robust 21 PLS models	2002:2025	The robust 21 PLS models defined in this work	2002:2046	The robust 21 PLS models defined in this work provided reliable tools for the rapid monitoring of juices' authenticity and/or deterioration.
29210453	5	23	theme	principal	861:869	arg1	analysis					881:888	A principal component analysis	859:888	A principal component analysis	859:888	A principal component analysis was performed on the FTIR spectra corresponding to the different treatments.
29210453	11	24	from	specifications	1773:1786	arg1	terms					1791:1795	terms	1791:1795	terms of authenticity, contamination and/or deterioration	1791:1847	In conclusion, FTIR spectroscopy appears as an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration.
29210453	14	25	theme	sugars	2239:2244	arg1	determination					2212:2224	the determination	2208:2224	the determination of different sugars in a single measurement without the need of pure sugars as standards	2208:2313	In this regard, FTIR associated to multivariate analysis enabled the determination of different sugars in a single measurement without the need of pure sugars as standards.
29210453	1	26	theme	strawberry	359:368	arg1	juices					370:375	strawberry juices	359:375	strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound)	359:463	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	0	27	theme	Sugars	109:114	arg1	Determination					92:104	the Simultaneous Determination	75:104	the Simultaneous Determination of Sugars and Fructans in Strawberry	75:141	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry Juices During Storage.
29210453	5	28	theme	different	945:953	arg1	treatments					955:964	the different treatments	941:964	the different treatments	941:964	A principal component analysis was performed on the FTIR spectra corresponding to the different treatments.
29210453	6	29	theme	variance	1018:1025	arg1	%					1009:1009	more than 94%	997:1009	more than 94% of the variance	997:1025	The groups observed explained more than 94% of the variance and were related to changes in the carbohydrate composition during storage.
29210453	6	29	theme	variance	1018:1025	arg1	variance					1018:1025	the variance	1014:1025	the variance	1014:1025	The groups observed explained more than 94% of the variance and were related to changes in the carbohydrate composition during storage.
29210453	10	30	theme	models	1582:1587	arg1	use					1567:1569	the use	1563:1569	the use of the PLS models to monitor the quality of strawberry juices in unknown samples	1563:1650	The mean of predicted values fitted nicely those obtained by HPLC (correlation and R2 > 0.97), thus supporting the use of the PLS models to monitor the quality of strawberry juices in unknown samples.
29210453	1	31	theme	simple	291:296	arg1	sugars					298:303	simple sugars	291:303	simple sugars	291:303	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	2	32	theme	spectral	475:482	arg1	differences					484:494	The main spectral differences	466:494	The main spectral differences	466:494	The main spectral differences were observed in the 1200 to 900 cm-1 region.
29210453	4	33	theme	preservation	771:782	arg1	treatment					784:792	any previous preservation treatment	758:792	any previous preservation treatment	758:792	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	7	34	theme	different	1109:1117	arg1	PLS					1148:1150	PLS	1148:1150	PLS	1148:1150	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	7	34	theme	different	1109:1117	arg1	models					1140:1145	different partial least square models	1109:1145	different partial least square models (PLS)	1109:1151	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	0	35	theme	Infrared	23:30	arg1	Spectroscopy					32:43	Infrared Spectroscopy	23:43	Infrared Spectroscopy	23:43	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry Juices During Storage.
29210453	7	36	theme	those	1233:1237	arg1	concentrations					1183:1196	the concentrations	1179:1196	the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5	1179:1308	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	11	37	theme	juice	1749:1753	arg1	formulations					1755:1766	juice formulations	1749:1766	juice formulations	1749:1766	In conclusion, FTIR spectroscopy appears as an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration.
29210453	1	38	theme	added	321:325	arg1	FOS					351:353	FOS	351:353	FOS	351:353	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	1	38	theme	added	321:325	arg1	fructooligosaccharides					327:348	exogenously added fructooligosaccharides	309:348	exogenously added fructooligosaccharides (FOS)	309:354	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	2	39	located	observed	501:508	arg2	differences					484:494	The main spectral differences	466:494	The main spectral differences	466:494	The main spectral differences were observed in the 1200 to 900 cm-1 region.
29210453	2	39	located	observed	501:508	arg1	region					534:539	the 1200 to 900 cm-1 region	513:539	the 1200 to 900 cm-1 region	513:539	The main spectral differences were observed in the 1200 to 900 cm-1 region.
29210453	6	40	theme	carbohydrate	1062:1073	arg1	composition					1075:1085	the carbohydrate composition	1058:1085	the carbohydrate composition during storage	1058:1100	The groups observed explained more than 94% of the variance and were related to changes in the carbohydrate composition during storage.
29210453	3	41	from	cm-1	621:624	arg1	bands					592:596	the typical bands	580:596	the typical bands at 1134, 1034, and 935 cm-1	580:624	The presence of FOS was identified by the typical bands at 1134, 1034, and 935 cm-1 .
29210453	1	42	dep	Fourier	182:188	arg1	transform					190:198	transform	190:198	transform mid-infrared spectroscopy (FTIR)-based method	190:244	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	10	43	theme	predicted	1464:1472	arg1	values					1474:1479	predicted values	1464:1479	predicted values	1464:1479	The mean of predicted values fitted nicely those obtained by HPLC (correlation and R2 > 0.97), thus supporting the use of the PLS models to monitor the quality of strawberry juices in unknown samples.
29210453	10	44	theme	strawberry	1615:1624	arg1	juices					1626:1631	strawberry juices	1615:1631	strawberry juices	1615:1631	The mean of predicted values fitted nicely those obtained by HPLC (correlation and R2 > 0.97), thus supporting the use of the PLS models to monitor the quality of strawberry juices in unknown samples.
29210453	15	45	theme	environmentally	2491:2505	arg1	way					2516:2518	an environmentally friendly way	2488:2518	an environmentally friendly way	2488:2518	This experimental simplicity supports the use of FTIR at the production line, and also contributes to save time in determining carbohydrates' composition and stability, in an environmentally friendly way.
29210453	4	46	theme	liquid	829:834	arg1	chromatography					836:849	high-performance liquid chromatography	812:849	high-performance liquid chromatography (HPLC)	812:856	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	4	46	theme	liquid	829:834	arg1	HPLC					852:855	HPLC	852:855	HPLC	852:855	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	7	47	with	glucose	1201:1207	arg1	degrees					1269:1275	degrees	1269:1275	degrees of polymerization within 3 and 5	1269:1308	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	10	48	from	quality	1604:1610	arg1	samples					1644:1650	unknown samples	1636:1650	unknown samples	1636:1650	The mean of predicted values fitted nicely those obtained by HPLC (correlation and R2 > 0.97), thus supporting the use of the PLS models to monitor the quality of strawberry juices in unknown samples.
29210453	7	49	with	sucrose	1210:1216	arg1	degrees					1269:1275	degrees	1269:1275	degrees of polymerization within 3 and 5	1269:1308	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	12	50	theme	food	1940:1943	arg1	industry					1945:1952	the food industry	1936:1952	the food industry	1936:1952	PRACTICAL APPLICATION FTIR spectroscopy provided a method potentially transferable to the food industry when associated with the multivariate analysis.
29210453	15	51	from	line	2388:2391	arg1	use					2358:2360	the use	2354:2360	the use of FTIR at the production line	2354:2391	This experimental simplicity supports the use of FTIR at the production line, and also contributes to save time in determining carbohydrates' composition and stability, in an environmentally friendly way.
29210453	7	52	with	fructose	1219:1226	arg1	degrees					1269:1275	degrees	1269:1275	degrees of polymerization within 3 and 5	1269:1308	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	14	53	theme	sugars	2295:2300	arg1	need					2282:2285	the need	2278:2285	the need of pure sugars as standards	2278:2313	In this regard, FTIR associated to multivariate analysis enabled the determination of different sugars in a single measurement without the need of pure sugars as standards.
29210453	3	54	theme	FOS	558:560	arg1	presence					546:553	The presence	542:553	The presence of FOS	542:560	The presence of FOS was identified by the typical bands at 1134, 1034, and 935 cm-1 .
29210453	11	55	theme	adequate	1700:1707	arg1	tool					1720:1723	an adequate analytical tool	1697:1723	an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration	1697:1847	In conclusion, FTIR spectroscopy appears as an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration.
29210453	8	56	theme	reference	1377:1385	arg1	method					1387:1392	reference method	1377:1392	reference method	1377:1392	The carbohydrates' concentrations determined by HPLC were used as reference method.
29210453	8	56	theme	reference	1377:1385	arg1	concentrations					1330:1343	The carbohydrates' concentrations	1311:1343	The carbohydrates' concentrations determined by HPLC	1311:1362	The carbohydrates' concentrations determined by HPLC were used as reference method.
29210453	13	57	theme	reliable	2057:2064	arg1	tools					2066:2070	reliable tools	2057:2070	reliable tools for the rapid monitoring of juices' authenticity and/or deterioration	2057:2140	The robust 21 PLS models defined in this work provided reliable tools for the rapid monitoring of juices' authenticity and/or deterioration.
29210453	1	58	theme	mid-infrared	200:211	arg1	FTIR					227:230	FTIR	227:230	FTIR	227:230	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	1	58	theme	mid-infrared	200:211	arg1	spectroscopy					213:224	mid-infrared spectroscopy	200:224	mid-infrared spectroscopy (FTIR)-based method	200:244	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	12	59	theme	APPLICATION	1860:1870	arg1	spectroscopy					1877:1888	PRACTICAL APPLICATION FTIR spectroscopy	1850:1888	PRACTICAL APPLICATION FTIR spectroscopy	1850:1888	PRACTICAL APPLICATION FTIR spectroscopy provided a method potentially transferable to the food industry when associated with the multivariate analysis.
29210453	2	60	theme	cm-1	529:532	arg1	region					534:539	the 1200 to 900 cm-1 region	513:539	the 1200 to 900 cm-1 region	513:539	The main spectral differences were observed in the 1200 to 900 cm-1 region.
29210453	13	61	dep	juices	2100:2105	arg1	deterioration					2128:2140	deterioration	2128:2140	deterioration	2128:2140	The robust 21 PLS models defined in this work provided reliable tools for the rapid monitoring of juices' authenticity and/or deterioration.
29210453	13	61	dep	juices	2100:2105	arg1	authenticity					2108:2119	authenticity	2108:2119	authenticity	2108:2119	The robust 21 PLS models defined in this work provided reliable tools for the rapid monitoring of juices' authenticity and/or deterioration.
29210453	5	62	theme	component	871:879	arg1	analysis					881:888	A principal component analysis	859:888	A principal component analysis	859:888	A principal component analysis was performed on the FTIR spectra corresponding to the different treatments.
29210453	1	63	theme	nonthermal	408:417	arg1	vanillin+ultrasound					444:462	vanillin+ultrasound	444:462	vanillin+ultrasound	444:462	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	1	63	theme	nonthermal	408:417	arg1	geraniol					431:438	geraniol	431:438	geraniol	431:438	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	1	63	theme	nonthermal	408:417	arg1	techniques					419:428	nonthermal techniques	408:428	nonthermal techniques (geraniol and vanillin+ultrasound)	408:463	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	9	64	theme	data	1446:1449	arg1	sets					1438:1441	independent sets	1426:1441	independent sets of data	1426:1449	The models were validated with independent sets of data.
29210453	9	64	theme	data	1446:1449	arg1	data					1446:1449	data	1446:1449	data	1446:1449	The models were validated with independent sets of data.
29210453	7	65	theme	polymerization	1280:1293	arg1	degrees					1269:1275	degrees	1269:1275	degrees of polymerization within 3 and 5	1269:1308	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	12	66	theme	multivariate	1979:1990	arg1	analysis					1992:1999	the multivariate analysis	1975:1999	the multivariate analysis	1975:1999	PRACTICAL APPLICATION FTIR spectroscopy provided a method potentially transferable to the food industry when associated with the multivariate analysis.
29210453	14	67	theme	single	2251:2256	arg1	measurement					2258:2268	a single measurement	2249:2268	a single measurement without the need of pure sugars as standards	2249:2313	In this regard, FTIR associated to multivariate analysis enabled the determination of different sugars in a single measurement without the need of pure sugars as standards.
29210453	0	68	theme	Simultaneous	79:90	arg1	Determination					92:104	the Simultaneous Determination	75:104	the Simultaneous Determination of Sugars and Fructans in Strawberry	75:141	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry Juices During Storage.
29210453	4	69	from	juices	736:741	arg1	increase					700:707	an increase	697:707	an increase of glucose and fructose in juices stored without any previous preservation treatment	697:792	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	4	70	theme	significant	646:656	arg1	decrease					658:665	a significant decrease	644:665	a significant decrease of sucrose	644:676	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	4	70	theme	significant	646:656	arg1	concomitant					682:692	concomitant	682:692	concomitant	682:692	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	15	71	theme	FTIR	2365:2368	arg1	use					2358:2360	the use	2354:2360	the use of FTIR at the production line	2354:2391	This experimental simplicity supports the use of FTIR at the production line, and also contributes to save time in determining carbohydrates' composition and stability, in an environmentally friendly way.
29210453	12	72	theme	PRACTICAL	1850:1858	arg1	APPLICATION					1860:1870	PRACTICAL APPLICATION	1850:1870	PRACTICAL APPLICATION FTIR spectroscopy	1850:1888	PRACTICAL APPLICATION FTIR spectroscopy provided a method potentially transferable to the food industry when associated with the multivariate analysis.
29210453	4	73	from	fructose	724:731	arg1	juices					736:741	juices	736:741	juices stored without any previous preservation treatment	736:792	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	4	74	theme	glucose	712:718	arg1	increase					700:707	an increase	697:707	an increase of glucose and fructose in juices stored without any previous preservation treatment	697:792	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	14	75	theme	different	2229:2237	arg1	sugars					2239:2244	different sugars	2229:2244	different sugars	2229:2244	In this regard, FTIR associated to multivariate analysis enabled the determination of different sugars in a single measurement without the need of pure sugars as standards.
29210453	4	76	from	glucose	712:718	arg1	juices					736:741	juices	736:741	juices stored without any previous preservation treatment	736:792	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	14	77	from	determination	2212:2224	arg1	measurement					2258:2268	a single measurement	2249:2268	a single measurement without the need of pure sugars as standards	2249:2313	In this regard, FTIR associated to multivariate analysis enabled the determination of different sugars in a single measurement without the need of pure sugars as standards.
29210453	4	78	theme	fructose	724:731	arg1	increase					700:707	an increase	697:707	an increase of glucose and fructose in juices stored without any previous preservation treatment	697:792	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	11	79	theme	contamination	1814:1826	arg1	terms					1791:1795	terms	1791:1795	terms of authenticity, contamination and/or deterioration	1791:1847	In conclusion, FTIR spectroscopy appears as an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration.
29210453	0	80	theme	Combined	2:9	arg1	Approach					11:18	A Combined Approach	0:18	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry	0:141	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry Juices During Storage.
29210453	11	81	dep	quick	1728:1732	arg1	assess					1734:1739	assess	1734:1739	assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration	1734:1847	In conclusion, FTIR spectroscopy appears as an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration.
29210453	4	82	from	increase	700:707	arg1	juices					736:741	juices	736:741	juices stored without any previous preservation treatment	736:792	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	7	83	theme	added	1254:1258	arg1	FOS					1260:1262	exogenously added FOS	1242:1262	exogenously added FOS	1242:1262	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	11	84	theme	authenticity	1800:1811	arg1	terms					1791:1795	terms	1791:1795	terms of authenticity, contamination and/or deterioration	1791:1847	In conclusion, FTIR spectroscopy appears as an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration.
29210453	7	85	theme	partial	1119:1125	arg1	PLS					1148:1150	PLS	1148:1150	PLS	1148:1150	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	7	85	theme	partial	1119:1125	arg1	models					1140:1145	different partial least square models	1109:1145	different partial least square models (PLS)	1109:1151	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	5	86	theme	FTIR	911:914	arg1	spectra					916:922	the FTIR spectra	907:922	the FTIR spectra corresponding to the different treatments	907:964	A principal component analysis was performed on the FTIR spectra corresponding to the different treatments.
29210453	14	87	theme	multivariate	2178:2189	arg1	analysis					2191:2198	multivariate analysis	2178:2198	multivariate analysis	2178:2198	In this regard, FTIR associated to multivariate analysis enabled the determination of different sugars in a single measurement without the need of pure sugars as standards.
29210453	10	88	theme	PLS	1578:1580	arg1	models					1582:1587	the PLS models	1574:1587	the PLS models	1574:1587	The mean of predicted values fitted nicely those obtained by HPLC (correlation and R2 > 0.97), thus supporting the use of the PLS models to monitor the quality of strawberry juices in unknown samples.
29210453	0	89	theme	Spectroscopy	32:43	arg1	Approach					11:18	A Combined Approach	0:18	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry	0:141	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry Juices During Storage.
29210453	2	90	theme	main	470:473	arg1	differences					484:494	The main spectral differences	466:494	The main spectral differences	466:494	The main spectral differences were observed in the 1200 to 900 cm-1 region.
29210453	4	91	theme	previous	762:769	arg1	treatment					784:792	any previous preservation treatment	758:792	any previous preservation treatment	758:792	During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC).
29210453	11	92	theme	deterioration	1835:1847	arg1	terms					1791:1795	terms	1791:1795	terms of authenticity, contamination and/or deterioration	1791:1847	In conclusion, FTIR spectroscopy appears as an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration.
29210453	0	93	theme	Multivariate	49:60	arg1	Analysis					62:69	Multivariate Analysis	49:69	Multivariate Analysis	49:69	A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry Juices During Storage.
29210453	7	94	theme	fructose	1219:1226	arg1	concentrations					1183:1196	the concentrations	1179:1196	the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5	1179:1308	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	7	95	theme	sucrose	1210:1216	arg1	concentrations					1183:1196	the concentrations	1179:1196	the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5	1179:1308	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	1	96	dep	14	397:398	arg1	to					394:395	to	394:395	to	394:395	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
29210453	6	97	from	changes	1047:1053	arg1	composition					1075:1085	the carbohydrate composition	1058:1085	the carbohydrate composition during storage	1058:1100	The groups observed explained more than 94% of the variance and were related to changes in the carbohydrate composition during storage.
29210453	10	98	theme	juices	1626:1631	arg1	quality					1604:1610	the quality	1600:1610	the quality of strawberry juices in unknown samples	1600:1650	The mean of predicted values fitted nicely those obtained by HPLC (correlation and R2 > 0.97), thus supporting the use of the PLS models to monitor the quality of strawberry juices in unknown samples.
29210453	15	99	theme	friendly	2507:2514	arg1	way					2516:2518	an environmentally friendly way	2488:2518	an environmentally friendly way	2488:2518	This experimental simplicity supports the use of FTIR at the production line, and also contributes to save time in determining carbohydrates' composition and stability, in an environmentally friendly way.
29210453	7	100	theme	glucose	1201:1207	arg1	concentrations					1183:1196	the concentrations	1179:1196	the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5	1179:1308	Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5.
29210453	10	101	theme	values	1474:1479	arg1	mean					1456:1459	The mean	1452:1459	The mean of predicted values	1452:1479	The mean of predicted values fitted nicely those obtained by HPLC (correlation and R2 > 0.97), thus supporting the use of the PLS models to monitor the quality of strawberry juices in unknown samples.
29210453	8	102	used	used	1369:1372	arg2	method					1387:1392	reference method	1377:1392	reference method	1377:1392	The carbohydrates' concentrations determined by HPLC were used as reference method.
29210453	8	102	used	used	1369:1372	arg2	concentrations					1330:1343	The carbohydrates' concentrations	1311:1343	The carbohydrates' concentrations determined by HPLC	1311:1362	The carbohydrates' concentrations determined by HPLC were used as reference method.
29210453	1	103	theme	-based	232:237	arg1	method					239:244	mid-infrared spectroscopy (FTIR)-based method	200:244	mid-infrared spectroscopy (FTIR)-based method	200:244	In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound).
26436669	3	0	theme	forest	313:318	arg1	litter					320:325	forest litter	313:325	forest litter	313:325	We therefore expect that crickets dwelling in forest litter have a limited supply of sugar-rich resource, and will perceive this and displace towards resource-supplemented sites.
26436669	3	1	contain	have	327:330	arg1	crickets					292:299	crickets	292:299	crickets dwelling in forest litter	292:325	We therefore expect that crickets dwelling in forest litter have a limited supply of sugar-rich resource, and will perceive this and displace towards resource-supplemented sites.
26436669	3	1	contain	have	327:330	arg2	supply					342:347	a limited supply	332:347	a limited supply of sugar-rich resource	332:370	We therefore expect that crickets dwelling in forest litter have a limited supply of sugar-rich resource, and will perceive this and displace towards resource-supplemented sites.
26436669	1	2	theme	forest	148:153	arg1	litter					155:160	forest litter	148:160	forest litter	148:160	Crickets are often found feeding on fallen fruits among forest litter.
26436669	4	3	theme	leaf	594:597	arg1	litter					599:604	leaf litter	594:604	leaf litter	594:604	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	0	4	theme	Local	75:79	arg1	Diversity					81:89	Local Diversity	75:89	Local Diversity	75:89	Aggregation of Cricket Activity in Response to Resource Addition Increases Local Diversity.
26436669	8	5	theme	resource	1372:1379	arg1	scarcity					1381:1388	resource scarcity	1372:1388	resource scarcity	1372:1388	We present evidence that the density of many species is limited by resource scarcity and, when resources are added, behavioral displacement promotes increased species packing and alters species composition.
26436669	7	6	theme	species	1246:1252	arg1	richness					1254:1261	species richness	1246:1261	species richness	1246:1261	When resource was experimentally increased, species richness increased due to behavioral displacement.
26436669	9	7	theme	species-abundance	1726:1742	arg1	characteristics					1744:1758	species-abundance characteristics	1726:1758	species-abundance characteristics	1726:1758	Further, our findings have technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics.
26436669	9	8	theme	diversity	1615:1623	arg1	efficiency					1587:1596	sampling efficiency	1578:1596	sampling efficiency of local cricket diversity	1578:1623	Further, our findings have technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics.
26436669	6	9	from	driver	1136:1141	arg1	forests					1193:1199	tropical forests	1184:1199	tropical forests	1184:1199	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	3	10	theme	sugar-rich	352:361	arg1	resource					363:370	sugar-rich resource	352:370	sugar-rich resource	352:370	We therefore expect that crickets dwelling in forest litter have a limited supply of sugar-rich resource, and will perceive this and displace towards resource-supplemented sites.
26436669	9	11	theme	sampling	1578:1585	arg1	efficiency					1587:1596	sampling efficiency	1578:1596	sampling efficiency of local cricket diversity	1578:1623	Further, our findings have technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics.
26436669	6	12	theme	short-term	1125:1134	arg1	driver					1136:1141	a short-term driver	1123:1141	a short-term driver of litter cricket community structure in tropical forests	1123:1199	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	6	12	theme	short-term	1125:1134	arg1	resource					1111:1118	sugar-rich resource	1100:1118	sugar-rich resource	1100:1118	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	6	13	theme	litter	1146:1151	arg1	structure					1171:1179	litter cricket community structure	1146:1179	litter cricket community structure	1146:1179	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	3	14	theme	limited	334:340	arg1	supply					342:347	a limited supply	332:347	a limited supply of sugar-rich resource	332:370	We therefore expect that crickets dwelling in forest litter have a limited supply of sugar-rich resource, and will perceive this and displace towards resource-supplemented sites.
26436669	4	15	theme	sugar	467:471	arg1	availability					473:484	sugar availability	467:484	sugar availability	467:484	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	6	16	from	litter	1023:1028	arg1	present					1005:1011	present	1005:1011	present	1005:1011	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	6	17	theme	sugar-rich	1100:1109	arg1	driver					1136:1141	a short-term driver	1123:1141	a short-term driver of litter cricket community structure in tropical forests	1123:1199	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	6	17	theme	sugar-rich	1100:1109	arg1	resource					1111:1118	sugar-rich resource	1100:1118	sugar-rich resource	1100:1118	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	6	18	theme	forest	1016:1021	arg1	litter					1023:1028	forest litter	1016:1028	forest litter	1016:1028	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	4	19	theme	cricket	494:500	arg1	richness					510:517	richness	510:517	richness	510:517	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	4	19	theme	cricket	494:500	arg1	species					502:508	cricket species	494:508	cricket species richness and abundance	494:531	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	4	19	theme	cricket	494:500	arg1	abundance					523:531	abundance	523:531	abundance	523:531	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	9	20	contain	have	1534:1537	arg1	findings					1525:1532	our findings	1521:1532	our findings	1521:1532	Further, our findings have technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics.
26436669	9	20	contain	have	1534:1537	arg2	applicability					1549:1561	technical applicability	1539:1561	technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics	1539:1758	Further, our findings have technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics.
26436669	8	21	theme	many	1345:1348	arg1	species					1350:1356	many species	1345:1356	many species	1345:1356	We present evidence that the density of many species is limited by resource scarcity and, when resources are added, behavioral displacement promotes increased species packing and alters species composition.
26436669	4	22	theme	pitfall	671:677	arg1	trapping					679:686	pitfall trapping	671:686	pitfall trapping	671:686	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	4	23	from	syrup	585:589	arg1	litter					599:604	leaf litter	594:604	leaf litter	594:604	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	6	24	theme	cricket	989:995	arg1	species					997:1003	the 13 cricket species	982:1003	the 13 cricket species present in forest litter	982:1028	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	5	25	theme	resource	909:916	arg1	addition					918:925	resource addition	909:925	resource addition	909:925	We found an asymptotic positive association between resource addition and species richness, and an interaction between resource addition and species identity on cricket abundance, which indicates differential effects of resource addition among cricket species.
26436669	3	26	theme	resource-supplemented	417:437	arg1	sites					439:443	resource-supplemented sites	417:443	resource-supplemented sites	417:443	We therefore expect that crickets dwelling in forest litter have a limited supply of sugar-rich resource, and will perceive this and displace towards resource-supplemented sites.
26436669	3	27	theme	resource	363:370	arg1	supply					342:347	a limited supply	332:347	a limited supply of sugar-rich resource	332:370	We therefore expect that crickets dwelling in forest litter have a limited supply of sugar-rich resource, and will perceive this and displace towards resource-supplemented sites.
26436669	6	28	theme	present	1005:1011	arg1	species					997:1003	the 13 cricket species	982:1003	the 13 cricket species present in forest litter	982:1028	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	8	29	theme	species	1350:1356	arg1	density					1334:1340	the density	1330:1340	the density of many species	1330:1356	We present evidence that the density of many species is limited by resource scarcity and, when resources are added, behavioral displacement promotes increased species packing and alters species composition.
26436669	6	30	theme	tropical	1184:1191	arg1	forests					1193:1199	tropical forests	1184:1199	tropical forests	1184:1199	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	0	31	theme	Cricket	15:21	arg1	Activity					23:30	Cricket Activity	15:30	Cricket Activity	15:30	Aggregation of Cricket Activity in Response to Resource Addition Increases Local Diversity.
26436669	6	32	theme	species	997:1003	arg1	species					997:1003	the 13 cricket species	982:1003	the 13 cricket species present in forest litter	982:1028	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	6	32	theme	species	997:1003	arg1	12					976:977	12	976:977	12	976:977	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	2	33	theme	other	174:178	arg1	resources					191:199	other sugar-rich resources	174:199	other sugar-rich resources	174:199	Fruits and other sugar-rich resources are not homogeneously distributed, nor are they always available.
26436669	0	34	dep	Addition	56:63	arg1	Response					35:42	Response	35:42	Response	35:42	Aggregation of Cricket Activity in Response to Resource Addition Increases Local Diversity.
26436669	9	35	theme	cricket	1607:1613	arg1	diversity					1615:1623	local cricket diversity	1601:1623	local cricket diversity	1601:1623	Further, our findings have technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics.
26436669	5	36	theme	cricket	850:856	arg1	abundance					858:866	cricket abundance	850:866	cricket abundance	850:866	We found an asymptotic positive association between resource addition and species richness, and an interaction between resource addition and species identity on cricket abundance, which indicates differential effects of resource addition among cricket species.
26436669	5	37	theme	resource	808:815	arg1	addition					817:824	resource addition	808:824	resource addition	808:824	We found an asymptotic positive association between resource addition and species richness, and an interaction between resource addition and species identity on cricket abundance, which indicates differential effects of resource addition among cricket species.
26436669	4	38	theme	sugarcane	575:583	arg1	syrup					585:589	sugarcane syrup	575:589	sugarcane syrup on leaf litter	575:604	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	5	39	theme	resource	741:748	arg1	addition					750:757	resource addition	741:757	resource addition	741:757	We found an asymptotic positive association between resource addition and species richness, and an interaction between resource addition and species identity on cricket abundance, which indicates differential effects of resource addition among cricket species.
26436669	5	40	theme	addition	918:925	arg1	effects					898:904	differential effects	885:904	differential effects of resource addition among cricket species	885:947	We found an asymptotic positive association between resource addition and species richness, and an interaction between resource addition and species identity on cricket abundance, which indicates differential effects of resource addition among cricket species.
26436669	9	41	theme	local	1601:1605	arg1	diversity					1615:1623	local cricket diversity	1601:1623	local cricket diversity	1601:1623	Further, our findings have technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics.
26436669	9	42	theme	local	1695:1699	arg1	drivers					1715:1721	local environmental drivers	1695:1721	local environmental drivers	1695:1721	Further, our findings have technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics.
26436669	4	43	theme	old-growth	536:545	arg1	forest					556:561	old-growth Atlantic forest	536:561	old-growth Atlantic forest	536:561	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	6	44	from	present	1005:1011	arg1	litter					1023:1028	forest litter	1016:1028	forest litter	1016:1028	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	0	45	theme	Activity	23:30	arg1	Aggregation					0:10	Aggregation	0:10	Aggregation of Cricket Activity in Response to Resource Addition	0:63	Aggregation of Cricket Activity in Response to Resource Addition Increases Local Diversity.
26436669	5	46	theme	differential	885:896	arg1	effects					898:904	differential effects	885:904	differential effects of resource addition among cricket species	885:947	We found an asymptotic positive association between resource addition and species richness, and an interaction between resource addition and species identity on cricket abundance, which indicates differential effects of resource addition among cricket species.
26436669	4	47	dep	species	502:508	arg1	richness					510:517	richness	510:517	richness	510:517	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	4	47	dep	species	502:508	arg1	species					502:508	cricket species	494:508	cricket species richness and abundance	494:531	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	4	47	dep	species	502:508	arg1	abundance					523:531	abundance	523:531	abundance	523:531	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	0	48	theme	Resource	47:54	arg1	Addition					56:63	Resource Addition	47:63	Resource Addition	47:63	Aggregation of Cricket Activity in Response to Resource Addition Increases Local Diversity.
26436669	6	49	theme	resource	1065:1072	arg1	scarcity					1074:1081	resource scarcity	1065:1081	resource scarcity	1065:1081	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	7	50	theme	behavioral	1280:1289	arg1	displacement					1291:1302	behavioral displacement	1280:1302	behavioral displacement	1280:1302	When resource was experimentally increased, species richness increased due to behavioral displacement.
26436669	8	51	theme	species	1464:1470	arg1	packing					1472:1478	increased species packing	1454:1478	increased species packing	1454:1478	We present evidence that the density of many species is limited by resource scarcity and, when resources are added, behavioral displacement promotes increased species packing and alters species composition.
26436669	6	52	theme	structure	1171:1179	arg1	driver					1136:1141	a short-term driver	1123:1141	a short-term driver of litter cricket community structure in tropical forests	1123:1199	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	6	52	theme	structure	1171:1179	arg1	resource					1111:1118	sugar-rich resource	1100:1118	sugar-rich resource	1100:1118	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	5	53	theme	species	830:836	arg1	identity					838:845	species identity	830:845	species identity	830:845	We found an asymptotic positive association between resource addition and species richness, and an interaction between resource addition and species identity on cricket abundance, which indicates differential effects of resource addition among cricket species.
26436669	8	54	theme	increased	1454:1462	arg1	packing					1472:1478	increased species packing	1454:1478	increased species packing	1454:1478	We present evidence that the density of many species is limited by resource scarcity and, when resources are added, behavioral displacement promotes increased species packing and alters species composition.
26436669	6	55	theme	community	1161:1169	arg1	structure					1171:1179	litter cricket community structure	1146:1179	litter cricket community structure	1146:1179	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	4	56	theme	Atlantic	547:554	arg1	forest					556:561	old-growth Atlantic forest	536:561	old-growth Atlantic forest	536:561	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	5	57	theme	asymptotic	701:710	arg1	association					721:731	an asymptotic positive association	698:731	an asymptotic positive association between resource addition and species richness	698:778	We found an asymptotic positive association between resource addition and species richness, and an interaction between resource addition and species identity on cricket abundance, which indicates differential effects of resource addition among cricket species.
26436669	6	58	attach	present	1005:1011	arg2	species					997:1003	the 13 cricket species	982:1003	the 13 cricket species present in forest litter	982:1028	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	6	58	attach	present	1005:1011	arg1	litter					1023:1028	forest litter	1016:1028	forest litter	1016:1028	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	9	59	theme	environmental	1701:1713	arg1	drivers					1715:1721	local environmental drivers	1695:1721	local environmental drivers	1695:1721	Further, our findings have technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics.
26436669	6	60	theme	cricket	1153:1159	arg1	structure					1171:1179	litter cricket community structure	1146:1179	litter cricket community structure	1146:1179	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	8	61	theme	behavioral	1421:1430	arg1	displacement					1432:1443	behavioral displacement	1421:1443	behavioral displacement	1421:1443	We present evidence that the density of many species is limited by resource scarcity and, when resources are added, behavioral displacement promotes increased species packing and alters species composition.
26436669	4	62	theme	increasing	618:627	arg1	availability					629:640	increasing availability	618:640	increasing availability	618:640	Here we evaluate how sugar availability affects cricket species richness and abundance in old-growth Atlantic forest by spraying sugarcane syrup on leaf litter, simulating increasing availability, and collecting crickets via pitfall trapping.
26436669	5	63	theme	positive	712:719	arg1	association					721:731	an asymptotic positive association	698:731	an asymptotic positive association between resource addition and species richness	698:778	We found an asymptotic positive association between resource addition and species richness, and an interaction between resource addition and species identity on cricket abundance, which indicates differential effects of resource addition among cricket species.
26436669	1	64	theme	fallen	128:133	arg1	fruits					135:140	fallen fruits	128:140	fallen fruits among forest litter	128:160	Crickets are often found feeding on fallen fruits among forest litter.
26436669	9	65	theme	technical	1539:1547	arg1	applicability					1549:1561	technical applicability	1539:1561	technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics	1539:1758	Further, our findings have technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics.
26436669	2	66	theme	sugar-rich	180:189	arg1	resources					191:199	other sugar-rich resources	174:199	other sugar-rich resources	174:199	Fruits and other sugar-rich resources are not homogeneously distributed, nor are they always available.
26436669	8	67	theme	species	1491:1497	arg1	composition					1499:1509	species composition	1491:1509	species composition	1491:1509	We present evidence that the density of many species is limited by resource scarcity and, when resources are added, behavioral displacement promotes increased species packing and alters species composition.
26436669	6	68	theme	low	1048:1050	arg1	densities					1052:1060	low densities	1048:1060	low densities	1048:1060	Our results indicate that 12 of the 13 cricket species present in forest litter are maintained at low densities by resource scarcity; this highlights sugar-rich resource as a short-term driver of litter cricket community structure in tropical forests.
26436669	5	69	dep	addition	750:757	arg1	richness					771:778	richness	771:778	richness	771:778	We found an asymptotic positive association between resource addition and species richness, and an interaction between resource addition and species identity on cricket abundance, which indicates differential effects of resource addition among cricket species.
26436669	9	70	theme	species	1655:1661	arg1	richness					1663:1670	species richness	1655:1670	species richness	1655:1670	Further, our findings have technical applicability for increasing sampling efficiency of local cricket diversity in studies aiming to estimate species richness, but with no regard to local environmental drivers or species-abundance characteristics.
26436669	5	71	theme	cricket	933:939	arg1	species					941:947	cricket species	933:947	cricket species	933:947	We found an asymptotic positive association between resource addition and species richness, and an interaction between resource addition and species identity on cricket abundance, which indicates differential effects of resource addition among cricket species.
24612537	0	0	theme	consequences	108:119	arg1	analysis					66:73	methyl-β-cyclodextrin and nystatin--comparative analysis	18:73	methyl-β-cyclodextrin and nystatin--comparative analysis of biochemical and physiological consequences for plants	18:130	Sterol binding by methyl-β-cyclodextrin and nystatin--comparative analysis of biochemical and physiological consequences for plants.
24612537	8	1	theme	sterol	1594:1599	arg1	content					1601:1607	the sterol content	1590:1607	the sterol content	1590:1607	Our results suggest that a decrease in the sterol content is, in itself, not sufficient to have deleterious effects on a cell.
24612537	1	2	theme	sterol	176:181	arg1	component					183:191	its sterol component	172:191	its sterol component	172:191	The dependence of membrane function on its sterol component has been intensively studied with model lipids and isolated animal membranes, but to a much lesser extent with plant membranes.
24612537	9	3	from	disturbance	1682:1692	arg1	content					1756:1762	the sterol content	1745:1762	the sterol content	1745:1762	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	0	4	theme	physiological	94:106	arg1	consequences					108:119	biochemical and physiological consequences	78:119	biochemical and physiological consequences for plants	78:130	Sterol binding by methyl-β-cyclodextrin and nystatin--comparative analysis of biochemical and physiological consequences for plants.
24612537	3	5	theme	cell	700:703	arg1	viability					705:713	cell viability	700:713	cell viability	700:713	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	4	6	theme	antibiotic	858:867	arg1	nystatin					869:876	The polyene antibiotic nystatin	846:876	The polyene antibiotic nystatin	846:876	The polyene antibiotic nystatin binds to endogenous sterols, forming so-called 'nystatin pores' or 'channels' in the membrane, and methyl-β-cyclodextrin has the capacity to sequester sterols in its hydrophobic core.
24612537	9	7	theme	decrease	1733:1740	arg1	disturbance					1682:1692	The disturbance	1678:1692	The disturbance of membrane integrity, rather than the decrease in the sterol content,	1678:1763	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	9	7	theme	decrease	1733:1740	arg1	responsible					1768:1778	responsible	1768:1778	responsible	1768:1778	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	7	8	theme	oxidative	1505:1513	arg1	stress					1515:1520	oxidative stress	1505:1520	oxidative stress	1505:1520	Gramicidin S considerably increased membrane permeability, caused oxidative stress, and reduced cell viability.
24612537	4	9	theme	hydrophobic	1044:1054	arg1	core					1056:1059	its hydrophobic core	1040:1059	its hydrophobic core	1040:1059	The polyene antibiotic nystatin binds to endogenous sterols, forming so-called 'nystatin pores' or 'channels' in the membrane, and methyl-β-cyclodextrin has the capacity to sequester sterols in its hydrophobic core.
24612537	3	10	theme	endogenous	826:835	arg1	sterols					837:843	endogenous sterols	826:843	endogenous sterols	826:843	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	4	11	contain	has	999:1001	arg1	methyl-β-cyclodextrin					977:997	methyl-β-cyclodextrin	977:997	methyl-β-cyclodextrin	977:997	The polyene antibiotic nystatin binds to endogenous sterols, forming so-called 'nystatin pores' or 'channels' in the membrane, and methyl-β-cyclodextrin has the capacity to sequester sterols in its hydrophobic core.
24612537	4	11	contain	has	999:1001	arg2	capacity					1007:1014	the capacity to sequester sterols in its hydrophobic core	1003:1059	the capacity to sequester sterols in its hydrophobic core	1003:1059	The polyene antibiotic nystatin binds to endogenous sterols, forming so-called 'nystatin pores' or 'channels' in the membrane, and methyl-β-cyclodextrin has the capacity to sequester sterols in its hydrophobic core.
24612537	3	12	theme	physiological	554:566	arg1	formation					607:615	formation	607:615	formation of autophagosomal vacuoles	607:642	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	12	theme	physiological	554:566	arg1	accumulation					593:604	H2O2 accumulation	588:604	H2O2 accumulation	588:604	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	12	theme	physiological	554:566	arg1	parameters					568:577	some physiological parameters	549:577	some physiological parameters	549:577	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	12	theme	physiological	554:566	arg1	expression					649:658	expression	649:658	expression of peroxidase and autophagic genes	649:693	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	5	13	theme	oxidative	1208:1216	arg1	status					1218:1223	oxidative status	1208:1223	oxidative status	1208:1223	Unexpectedly, although application of both methyl-β-cyclodextrin and nystatin reduced the sterol content, their effects on membrane permeability, oxidative status and autophagosome formation in roots differed dramatically.
24612537	9	14	theme	sterol	1749:1754	arg1	content					1756:1762	the sterol content	1745:1762	the sterol content	1745:1762	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	3	15	theme	wheat	731:735	arg1	seedlings					760:768	wheat (Triticum aestivum L.) seedlings	731:768	wheat (Triticum aestivum L.) seedlings	731:768	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	16	theme	membrane	517:524	arg1	permeability					526:537	membrane permeability	517:537	membrane permeability for ions	517:546	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	17	theme	lipid	498:502	arg1	composition					504:514	membrane lipid composition	489:514	membrane lipid composition	489:514	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	2	18	contain	have	370:373	arg2	effect					384:389	a strong effect	375:389	a strong effect on membrane activity	375:410	Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences.
24612537	2	18	contain	have	370:373	arg1	sterols					340:346	Depleting membrane sterols	321:346	Depleting membrane sterols	321:346	Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences.
24612537	1	19	theme	model	227:231	arg1	lipids					233:238	model lipids	227:238	model lipids	227:238	The dependence of membrane function on its sterol component has been intensively studied with model lipids and isolated animal membranes, but to a much lesser extent with plant membranes.
24612537	6	20	theme	S	1357:1357	arg1	effects					1320:1326	the effects	1316:1326	the effects of the antibiotic gramicidin S, which does not bind to sterols but forms nonspecific channels in the membrane	1316:1436	For comparison, we also tested the effects of the antibiotic gramicidin S, which does not bind to sterols but forms nonspecific channels in the membrane.
24612537	7	21	theme	Gramicidin	1439:1448	arg1	S					1450:1450	Gramicidin S	1439:1450	Gramicidin S	1439:1450	Gramicidin S considerably increased membrane permeability, caused oxidative stress, and reduced cell viability.
24612537	5	22	theme	methyl-β-cyclodextrin	1105:1125	arg1	application					1085:1095	application	1085:1095	application of both methyl-β-cyclodextrin and nystatin	1085:1138	Unexpectedly, although application of both methyl-β-cyclodextrin and nystatin reduced the sterol content, their effects on membrane permeability, oxidative status and autophagosome formation in roots differed dramatically.
24612537	5	23	theme	autophagosome	1229:1241	arg1	formation					1243:1251	autophagosome formation	1229:1251	autophagosome formation	1229:1251	Unexpectedly, although application of both methyl-β-cyclodextrin and nystatin reduced the sterol content, their effects on membrane permeability, oxidative status and autophagosome formation in roots differed dramatically.
24612537	2	24	theme	membrane	394:401	arg1	activity					403:410	membrane activity	394:410	membrane activity	394:410	Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences.
24612537	5	25	theme	membrane	1185:1192	arg1	permeability					1194:1205	membrane permeability	1185:1205	membrane permeability	1185:1205	Unexpectedly, although application of both methyl-β-cyclodextrin and nystatin reduced the sterol content, their effects on membrane permeability, oxidative status and autophagosome formation in roots differed dramatically.
24612537	4	26	theme	polyene	850:856	arg1	nystatin					869:876	The polyene antibiotic nystatin	846:876	The polyene antibiotic nystatin	846:876	The polyene antibiotic nystatin binds to endogenous sterols, forming so-called 'nystatin pores' or 'channels' in the membrane, and methyl-β-cyclodextrin has the capacity to sequester sterols in its hydrophobic core.
24612537	2	27	theme	membrane	331:338	arg1	sterols					340:346	Depleting membrane sterols	321:346	Depleting membrane sterols	321:346	Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences.
24612537	4	28	theme	endogenous	887:896	arg1	sterols					898:904	endogenous sterols	887:904	endogenous sterols	887:904	The polyene antibiotic nystatin binds to endogenous sterols, forming so-called 'nystatin pores' or 'channels' in the membrane, and methyl-β-cyclodextrin has the capacity to sequester sterols in its hydrophobic core.
24612537	5	29	theme	nystatin	1131:1138	arg1	application					1085:1095	application	1085:1095	application of both methyl-β-cyclodextrin and nystatin	1085:1138	Unexpectedly, although application of both methyl-β-cyclodextrin and nystatin reduced the sterol content, their effects on membrane permeability, oxidative status and autophagosome formation in roots differed dramatically.
24612537	1	30	theme	lesser	285:290	arg1	extent					292:297	a much lesser extent	278:297	a much lesser extent with plant membranes	278:318	The dependence of membrane function on its sterol component has been intensively studied with model lipids and isolated animal membranes, but to a much lesser extent with plant membranes.
24612537	5	31	from	effects	1174:1180	arg1	roots					1256:1260	roots	1256:1260	roots	1256:1260	Unexpectedly, although application of both methyl-β-cyclodextrin and nystatin reduced the sterol content, their effects on membrane permeability, oxidative status and autophagosome formation in roots differed dramatically.
24612537	5	31	from	effects	1174:1180	arg1	status					1218:1223	oxidative status	1208:1223	oxidative status	1208:1223	Unexpectedly, although application of both methyl-β-cyclodextrin and nystatin reduced the sterol content, their effects on membrane permeability, oxidative status and autophagosome formation in roots differed dramatically.
24612537	5	31	from	effects	1174:1180	arg1	formation					1243:1251	autophagosome formation	1229:1251	autophagosome formation	1229:1251	Unexpectedly, although application of both methyl-β-cyclodextrin and nystatin reduced the sterol content, their effects on membrane permeability, oxidative status and autophagosome formation in roots differed dramatically.
24612537	5	31	from	effects	1174:1180	arg1	permeability					1194:1205	membrane permeability	1185:1205	membrane permeability	1185:1205	Unexpectedly, although application of both methyl-β-cyclodextrin and nystatin reduced the sterol content, their effects on membrane permeability, oxidative status and autophagosome formation in roots differed dramatically.
24612537	1	32	from	dependence	137:146	arg1	component					183:191	its sterol component	172:191	its sterol component	172:191	The dependence of membrane function on its sterol component has been intensively studied with model lipids and isolated animal membranes, but to a much lesser extent with plant membranes.
24612537	2	33	theme	Depleting	321:329	arg1	sterols					340:346	Depleting membrane sterols	321:346	Depleting membrane sterols	321:346	Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences.
24612537	6	34	theme	antibiotic	1335:1344	arg1	S					1357:1357	the antibiotic gramicidin S	1331:1357	the antibiotic gramicidin S	1331:1357	For comparison, we also tested the effects of the antibiotic gramicidin S, which does not bind to sterols but forms nonspecific channels in the membrane.
24612537	0	35	theme	methyl-β-cyclodextrin	18:38	arg1	analysis					66:73	methyl-β-cyclodextrin and nystatin--comparative analysis	18:73	methyl-β-cyclodextrin and nystatin--comparative analysis of biochemical and physiological consequences for plants	18:130	Sterol binding by methyl-β-cyclodextrin and nystatin--comparative analysis of biochemical and physiological consequences for plants.
24612537	6	36	theme	gramicidin	1346:1355	arg1	S					1357:1357	the antibiotic gramicidin S	1331:1357	the antibiotic gramicidin S	1331:1357	For comparison, we also tested the effects of the antibiotic gramicidin S, which does not bind to sterols but forms nonspecific channels in the membrane.
24612537	1	37	with	extent	292:297	arg1	membranes					310:318	plant membranes	304:318	plant membranes	304:318	The dependence of membrane function on its sterol component has been intensively studied with model lipids and isolated animal membranes, but to a much lesser extent with plant membranes.
24612537	2	38	theme	physiological	429:441	arg1	consequences					443:454	harmful physiological consequences	421:454	harmful physiological consequences	421:454	Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences.
24612537	3	39	theme	H2O2	588:591	arg1	accumulation					593:604	H2O2 accumulation	588:604	H2O2 accumulation	588:604	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	4	40	theme	so-called	915:923	arg1	pores					935:939	nystatin pores	926:939	so-called 'nystatin pores'	915:940	The polyene antibiotic nystatin binds to endogenous sterols, forming so-called 'nystatin pores' or 'channels' in the membrane, and methyl-β-cyclodextrin has the capacity to sequester sterols in its hydrophobic core.
24612537	3	41	theme	peroxidase	663:672	arg1	genes					689:693	peroxidase and autophagic genes	663:693	genes	689:693	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	2	42	theme	harmful	421:427	arg1	consequences					443:454	harmful physiological consequences	421:454	harmful physiological consequences	421:454	Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences.
24612537	0	43	theme	nystatin--comparative	44:64	arg1	analysis					66:73	methyl-β-cyclodextrin and nystatin--comparative analysis	18:73	methyl-β-cyclodextrin and nystatin--comparative analysis of biochemical and physiological consequences for plants	18:130	Sterol binding by methyl-β-cyclodextrin and nystatin--comparative analysis of biochemical and physiological consequences for plants.
24612537	1	44	theme	plant	304:308	arg1	membranes					310:318	plant membranes	304:318	plant membranes	304:318	The dependence of membrane function on its sterol component has been intensively studied with model lipids and isolated animal membranes, but to a much lesser extent with plant membranes.
24612537	6	45	theme	nonspecific	1401:1411	arg1	channels					1413:1420	nonspecific channels	1401:1420	nonspecific channels	1401:1420	For comparison, we also tested the effects of the antibiotic gramicidin S, which does not bind to sterols but forms nonspecific channels in the membrane.
24612537	7	46	theme	membrane	1475:1482	arg1	permeability					1484:1495	membrane permeability	1475:1495	membrane permeability	1475:1495	Gramicidin S considerably increased membrane permeability, caused oxidative stress, and reduced cell viability.
24612537	4	47	theme	nystatin	926:933	arg1	pores					935:939	nystatin pores	926:939	so-called 'nystatin pores'	915:940	The polyene antibiotic nystatin binds to endogenous sterols, forming so-called 'nystatin pores' or 'channels' in the membrane, and methyl-β-cyclodextrin has the capacity to sequester sterols in its hydrophobic core.
24612537	9	48	from	decrease	1733:1740	arg1	content					1756:1762	the sterol content	1745:1762	the sterol content	1745:1762	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	9	49	theme	sterol-binding	1800:1813	arg1	compounds					1815:1823	sterol-binding compounds	1800:1823	sterol-binding compounds	1800:1823	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	5	50	theme	sterol	1152:1157	arg1	content					1159:1165	the sterol content	1148:1165	the sterol content	1148:1165	Unexpectedly, although application of both methyl-β-cyclodextrin and nystatin reduced the sterol content, their effects on membrane permeability, oxidative status and autophagosome formation in roots differed dramatically.
24612537	9	51	theme	membrane	1697:1704	arg1	integrity					1706:1714	membrane integrity	1697:1714	membrane integrity	1697:1714	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	7	52	theme	cell	1535:1538	arg1	viability					1540:1548	cell viability	1535:1548	cell viability	1535:1548	Gramicidin S considerably increased membrane permeability, caused oxidative stress, and reduced cell viability.
24612537	2	53	from	effect	384:389	arg1	activity					403:410	membrane activity	394:410	membrane activity	394:410	Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences.
24612537	9	54	theme	compounds	1815:1823	arg1	toxicity					1788:1795	the toxicity	1784:1795	the toxicity of sterol-binding compounds	1784:1823	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	3	55	theme	seedlings	760:768	arg1	roots					722:726	the roots	718:726	the roots of wheat (Triticum aestivum L.) seedlings	718:768	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	56	theme	membrane	489:496	arg1	composition					504:514	membrane lipid composition	489:514	membrane lipid composition	489:514	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	8	57	contain	have	1642:1645	arg1	decrease					1578:1585	a decrease	1576:1585	a decrease in the sterol content	1576:1607	Our results suggest that a decrease in the sterol content is, in itself, not sufficient to have deleterious effects on a cell.
24612537	8	57	contain	have	1642:1645	arg1	sufficient					1628:1637	sufficient	1628:1637	sufficient	1628:1637	Our results suggest that a decrease in the sterol content is, in itself, not sufficient to have deleterious effects on a cell.
24612537	8	57	contain	have	1642:1645	arg2	effects					1659:1665	deleterious effects	1647:1665	deleterious effects	1647:1665	Our results suggest that a decrease in the sterol content is, in itself, not sufficient to have deleterious effects on a cell.
24612537	9	58	from	content	1756:1762	arg1	disturbance					1682:1692	The disturbance	1678:1692	The disturbance of membrane integrity, rather than the decrease in the sterol content,	1678:1763	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	9	58	from	content	1756:1762	arg1	responsible					1768:1778	responsible	1768:1778	responsible	1768:1778	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	8	59	from	itself	1616:1621	arg1	decrease					1578:1585	a decrease	1576:1585	a decrease in the sterol content	1576:1607	Our results suggest that a decrease in the sterol content is, in itself, not sufficient to have deleterious effects on a cell.
24612537	8	59	from	itself	1616:1621	arg1	sufficient					1628:1637	sufficient	1628:1637	sufficient	1628:1637	Our results suggest that a decrease in the sterol content is, in itself, not sufficient to have deleterious effects on a cell.
24612537	2	60	contain	have	416:419	arg2	consequences					443:454	harmful physiological consequences	421:454	harmful physiological consequences	421:454	Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences.
24612537	2	60	contain	have	416:419	arg1	sterols					340:346	Depleting membrane sterols	321:346	Depleting membrane sterols	321:346	Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences.
24612537	9	61	theme	integrity	1706:1714	arg1	disturbance					1682:1692	The disturbance	1678:1692	The disturbance of membrane integrity, rather than the decrease in the sterol content,	1678:1763	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	9	61	theme	integrity	1706:1714	arg1	responsible					1768:1778	responsible	1768:1778	responsible	1768:1778	The disturbance of membrane integrity, rather than the decrease in the sterol content, is responsible for the toxicity of sterol-binding compounds.
24612537	8	62	from	decrease	1578:1585	arg1	content					1601:1607	the sterol content	1590:1607	the sterol content	1590:1607	Our results suggest that a decrease in the sterol content is, in itself, not sufficient to have deleterious effects on a cell.
24612537	1	63	theme	membrane	151:158	arg1	function					160:167	membrane function	151:167	membrane function	151:167	The dependence of membrane function on its sterol component has been intensively studied with model lipids and isolated animal membranes, but to a much lesser extent with plant membranes.
24612537	1	64	theme	isolated	244:251	arg1	membranes					260:268	isolated animal membranes	244:268	isolated animal membranes	244:268	The dependence of membrane function on its sterol component has been intensively studied with model lipids and isolated animal membranes, but to a much lesser extent with plant membranes.
24612537	8	65	from	sufficient	1628:1637	arg1	itself					1616:1621	itself	1616:1621	itself	1616:1621	Our results suggest that a decrease in the sterol content is, in itself, not sufficient to have deleterious effects on a cell.
24612537	3	66	theme	autophagic	678:687	arg1	genes					689:693	peroxidase and autophagic genes	663:693	genes	689:693	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	1	67	theme	function	160:167	arg1	dependence					137:146	The dependence	133:146	The dependence of membrane function on its sterol component	133:191	The dependence of membrane function on its sterol component has been intensively studied with model lipids and isolated animal membranes, but to a much lesser extent with plant membranes.
24612537	8	68	theme	deleterious	1647:1657	arg1	effects					1659:1665	deleterious effects	1647:1665	deleterious effects	1647:1665	Our results suggest that a decrease in the sterol content is, in itself, not sufficient to have deleterious effects on a cell.
24612537	1	69	theme	animal	253:258	arg1	membranes					260:268	isolated animal membranes	244:268	isolated animal membranes	244:268	The dependence of membrane function on its sterol component has been intensively studied with model lipids and isolated animal membranes, but to a much lesser extent with plant membranes.
24612537	3	70	theme	genes	689:693	arg1	formation					607:615	formation	607:615	formation of autophagosomal vacuoles	607:642	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	70	theme	genes	689:693	arg1	accumulation					593:604	H2O2 accumulation	588:604	H2O2 accumulation	588:604	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	70	theme	genes	689:693	arg1	expression					649:658	expression	649:658	expression of peroxidase and autophagic genes	649:693	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	71	theme	autophagosomal	620:633	arg1	vacuoles					635:642	autophagosomal vacuoles	620:642	autophagosomal vacuoles	620:642	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	2	72	theme	strong	377:382	arg1	effect					384:389	a strong effect	375:389	a strong effect on membrane activity	375:410	Depleting membrane sterols could be predicted to have a strong effect on membrane activity and have harmful physiological consequences.
24612537	0	73	theme	biochemical	78:88	arg1	consequences					108:119	biochemical and physiological consequences	78:119	biochemical and physiological consequences for plants	78:130	Sterol binding by methyl-β-cyclodextrin and nystatin--comparative analysis of biochemical and physiological consequences for plants.
24612537	3	74	dep	Triticum	738:745	arg1	L.					756:757	Triticum aestivum L.	738:757	Triticum aestivum L.	738:757	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	75	theme	vacuoles	635:642	arg1	formation					607:615	formation	607:615	formation of autophagosomal vacuoles	607:642	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	75	theme	vacuoles	635:642	arg1	accumulation					593:604	H2O2 accumulation	588:604	H2O2 accumulation	588:604	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	75	theme	vacuoles	635:642	arg1	expression					649:658	expression	649:658	expression of peroxidase and autophagic genes	649:693	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
24612537	3	76	theme	agents	793:798	arg1	presence					777:784	the presence	773:784	the presence of two agents that specifically bind to endogenous sterols	773:843	In this study, we characterized membrane lipid composition, membrane permeability for ions, some physiological parameters, such as H2O2 accumulation, formation of autophagosomal vacuoles, and expression of peroxidase and autophagic genes, and cell viability in the roots of wheat (Triticum aestivum L.) seedlings in the presence of two agents that specifically bind to endogenous sterols.
26413620	0	0	theme	Intracellular	84:96	arg1	Transporters					98:109	Gene Intracellular Transporters	79:109	Gene Intracellular Transporters	79:109	Functionalized Graphene Oxide with Hepatocyte Targeting as Anti-Tumor Drug and Gene Intracellular Transporters.
26413620	0	1	theme	Gene	79:82	arg1	Transporters					98:109	Gene Intracellular Transporters	79:109	Gene Intracellular Transporters	79:109	Functionalized Graphene Oxide with Hepatocyte Targeting as Anti-Tumor Drug and Gene Intracellular Transporters.
26413620	1	2	theme	anti-tumor	177:186	arg1	drug					188:191	anti-tumor drug	177:191	anti-tumor drug	177:191	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	6	3	theme	low	1253:1255	arg1	cytotoxicity					1257:1268	low cytotoxicity	1253:1268	low cytotoxicity	1253:1268	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	4	4	theme	doxorubicin	834:844	arg1	Dox					856:858	Dox	856:858	Dox	856:858	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	4	4	theme	doxorubicin	834:844	arg1	chloride					846:853	doxorubicin chloride	834:853	doxorubicin chloride (Dox)	834:859	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	3	5	dep	this	474:477	arg1	GO					494:495	GO	494:495	GO	494:495	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	6	theme	this	474:477	arg1	uptakes					463:469	The in vitro cells uptakes	444:469	The in vitro cells uptakes of this functionalized GO	444:495	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	6	7	theme	combined	1333:1340	arg1	therapy					1342:1348	gene combined therapy	1328:1348	gene combined therapy	1328:1348	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	5	8	theme	QGY-7703	1071:1078	arg1	cells					1080:1084	QGY-7703 cells	1071:1084	QGY-7703 cells	1071:1084	The toxicity of GO-LCO+ before and after loading with Dox toward QGY-7703 cells was further investigated.
26413620	6	9	theme	genes	1242:1246	arg1	co-delivery					1206:1216	hepatocyte targeted co-delivery	1186:1216	hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy	1186:1348	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	6	10	dep	functionalized	1136:1149	arg1	GO					1151:1152	GO	1151:1152	GO	1151:1152	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	1	11	theme	drug	188:191	arg1	delivery					165:172	the hepatocyte-specific targeted delivery	132:172	the hepatocyte-specific targeted delivery of anti-tumor drug and gene	132:200	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	4	12	theme	mg/ml	941:945	arg1	concentration					919:931	the initial Dox concentration	903:931	the initial Dox concentration of 0.45 mg/ml	903:945	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	4	13	theme	chloride	846:853	arg1	efficiency					820:829	The loading efficiency	808:829	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg	808:885	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	1	14	contain	containing	287:296	arg1	GO-LCO					279:284	GO-LCO	279:284	GO-LCO	279:284	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	1	14	contain	containing	287:296	arg1	oxides					271:276	lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides	203:276	lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+)	203:333	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	1	14	contain	containing	287:296	arg2	ammonium					309:316	quaternary ammonium groups (GO-LCO+)	298:333	quaternary ammonium groups (GO-LCO+)	298:333	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	0	15	theme	Graphene	15:22	arg1	Oxide					24:28	Graphene Oxide	15:28	Graphene Oxide	15:28	Functionalized Graphene Oxide with Hepatocyte Targeting as Anti-Tumor Drug and Gene Intracellular Transporters.
26413620	4	16	theme	Dox	962:964	arg1	release					951:957	release	951:957	release of Dox on GO-LCO+	951:975	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	6	17	theme	drugs	1232:1236	arg1	co-delivery					1206:1216	hepatocyte targeted co-delivery	1186:1216	hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy	1186:1348	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	3	18	theme	fluorescein	563:573	arg1	FAM-DNA					601:607	FAM-DNA	601:607	FAM-DNA	601:607	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	18	theme	fluorescein	563:573	arg1	sequence					591:598	fluorescein FAM-labeled DNA sequence	563:598	fluorescein FAM-labeled DNA sequence (FAM-DNA)	563:608	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	6	19	theme	future	1285:1290	arg1	applications					1292:1303	future applications	1285:1303	future applications in anticancer drug and gene combined therapy	1285:1348	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	1	20	theme	gene	197:200	arg1	delivery					165:172	the hepatocyte-specific targeted delivery	132:172	the hepatocyte-specific targeted delivery of anti-tumor drug and gene	132:200	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	6	21	theme	anti-tumor	1221:1230	arg1	drugs					1232:1236	anti-tumor drugs	1221:1236	anti-tumor drugs	1221:1236	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	3	22	theme	FAM-labeled	575:585	arg1	FAM-DNA					601:607	FAM-DNA	601:607	FAM-DNA	601:607	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	22	theme	FAM-labeled	575:585	arg1	sequence					591:598	fluorescein FAM-labeled DNA sequence	563:598	fluorescein FAM-labeled DNA sequence (FAM-DNA)	563:608	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	1	23	theme	quaternary	298:307	arg1	ammonium					309:316	quaternary ammonium groups (GO-LCO+)	298:333	quaternary ammonium groups (GO-LCO+)	298:333	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	4	24	theme	initial	907:913	arg1	concentration					919:931	the initial Dox concentration	903:931	the initial Dox concentration of 0.45 mg/ml	903:945	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	6	25	theme	hepatocyte	1186:1195	arg1	co-delivery					1206:1216	hepatocyte targeted co-delivery	1186:1216	hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy	1186:1348	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	2	26	dep	formation	354:362	arg1	The					350:352	The	350:352	The	350:352	The formation and composition of GO-LCO+ were confirmed by FTIR, AFM, TGA and zeta-potential.
26413620	3	27	with	cells	639:643	arg1	efficiency					668:677	higher efficiency	661:677	higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification	661:805	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	1	28	theme	lactosylated	203:214	arg1	GO-LCO					279:284	GO-LCO	279:284	GO-LCO	279:284	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	1	28	theme	lactosylated	203:214	arg1	oxides					271:276	lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides	203:276	lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+)	203:333	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	3	29	dep	charged	695:701	arg1	functionalized					733:746	functionalized	733:746	functionalized	733:746	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	1	30	theme	targeted	156:163	arg1	delivery					165:172	the hepatocyte-specific targeted delivery	132:172	the hepatocyte-specific targeted delivery of anti-tumor drug and gene	132:200	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	3	31	theme	graphene	748:755	arg1	oxides					757:762	positively charged chitosan oligosaccharide (CO) functionalized graphene oxides	684:762	positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification	684:805	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	31	theme	graphene	748:755	arg1	GO-CO+					765:770	GO-CO+	765:770	GO-CO+	765:770	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	6	32	theme	anticancer	1308:1317	arg1	drug					1319:1322	anticancer drug	1308:1322	anticancer drug	1308:1322	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	5	33	with	loading	1047:1053	arg1	Dox					1060:1062	Dox	1060:1062	Dox	1060:1062	The toxicity of GO-LCO+ before and after loading with Dox toward QGY-7703 cells was further investigated.
26413620	3	34	theme	in	448:449	arg1	uptakes					463:469	The in vitro cells uptakes	444:469	The in vitro cells uptakes of this functionalized GO	444:495	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	0	35	theme	Hepatocyte	35:44	arg1	Targeting					46:54	Hepatocyte Targeting	35:54	Hepatocyte Targeting	35:54	Functionalized Graphene Oxide with Hepatocyte Targeting as Anti-Tumor Drug and Gene Intracellular Transporters.
26413620	4	36	theme	Dox	915:917	arg1	concentration					919:931	the initial Dox concentration	903:931	the initial Dox concentration of 0.45 mg/ml	903:945	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	3	37	theme	carcinoma	629:637	arg1	QGY-7703					646:653	QGY-7703	646:653	QGY-7703	646:653	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	37	theme	carcinoma	629:637	arg1	cells					639:643	human hepatic carcinoma cells	615:643	human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification	615:805	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	38	theme	acid	789:792	arg1	modification					794:805	Lactose acid modification	781:805	Lactose acid modification	781:805	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	6	39	theme	gene	1328:1331	arg1	therapy					1342:1348	gene combined therapy	1328:1348	gene combined therapy	1328:1348	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	0	40	theme	Anti-Tumor	59:68	arg1	Drug					70:73	Anti-Tumor Drug	59:73	Anti-Tumor Drug	59:73	Functionalized Graphene Oxide with Hepatocyte Targeting as Anti-Tumor Drug and Gene Intracellular Transporters.
26413620	3	41	theme	Lactose	781:787	arg1	modification					794:805	Lactose acid modification	781:805	Lactose acid modification	781:805	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	42	theme	DNA	587:589	arg1	FAM-DNA					601:607	FAM-DNA	601:607	FAM-DNA	601:607	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	42	theme	DNA	587:589	arg1	sequence					591:598	fluorescein FAM-labeled DNA sequence	563:598	fluorescein FAM-labeled DNA sequence (FAM-DNA)	563:608	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	4	43	theme	strong	984:989	arg1	dependence					994:1003	strong pH dependence	984:1003	strong pH dependence	984:1003	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	6	44	from	applications	1292:1303	arg1	therapy					1342:1348	gene combined therapy	1328:1348	gene combined therapy	1328:1348	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	6	44	from	applications	1292:1303	arg1	drug					1319:1322	anticancer drug	1308:1322	anticancer drug	1308:1322	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	3	45	theme	charged	695:701	arg1	oxides					757:762	positively charged chitosan oligosaccharide (CO) functionalized graphene oxides	684:762	positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification	684:805	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	45	theme	charged	695:701	arg1	GO-CO+					765:770	GO-CO+	765:770	GO-CO+	765:770	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	46	theme	higher	661:666	arg1	efficiency					668:677	higher efficiency	661:677	higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification	661:805	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	5	47	theme	GO-LCO+	1022:1028	arg1	toxicity					1010:1017	The toxicity	1006:1017	The toxicity of GO-LCO+ before and after loading with Dox toward QGY-7703 cells	1006:1084	The toxicity of GO-LCO+ before and after loading with Dox toward QGY-7703 cells was further investigated.
26413620	4	48	from	efficiency	820:829	arg1	GO-LCO+					864:870	GO-LCO+	864:870	GO-LCO+ with 477 µg/mg	864:885	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	3	49	dep	in	448:449	arg1	vitro					451:455	vitro	451:455	vitro	451:455	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	50	theme	human	615:619	arg1	QGY-7703					646:653	QGY-7703	646:653	QGY-7703	646:653	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	50	theme	human	615:619	arg1	cells					639:643	human hepatic carcinoma cells	615:643	human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification	615:805	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	4	51	theme	loading	812:818	arg1	efficiency					820:829	The loading efficiency	808:829	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg	808:885	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	6	52	with	co-delivery	1206:1216	arg1	cytotoxicity					1257:1268	low cytotoxicity	1253:1268	low cytotoxicity	1253:1268	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	6	53	theme	promising	1271:1279	arg1	cytotoxicity					1257:1268	low cytotoxicity	1253:1268	low cytotoxicity	1253:1268	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	6	54	theme	targeted	1197:1204	arg1	co-delivery					1206:1216	hepatocyte targeted co-delivery	1186:1216	hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy	1186:1348	Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
26413620	3	55	theme	hepatic	621:627	arg1	QGY-7703					646:653	QGY-7703	646:653	QGY-7703	646:653	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	3	55	theme	hepatic	621:627	arg1	cells					639:643	human hepatic carcinoma cells	615:643	human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification	615:805	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	1	56	theme	hepatocyte-specific	136:154	arg1	delivery					165:172	the hepatocyte-specific targeted delivery	132:172	the hepatocyte-specific targeted delivery of anti-tumor drug and gene	132:200	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	4	57	with	GO-LCO+	864:870	arg1	µg/mg					881:885	477 µg/mg	877:885	477 µg/mg	877:885	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	3	58	theme	cells	457:461	arg1	uptakes					463:469	The in vitro cells uptakes	444:469	The in vitro cells uptakes of this functionalized GO	444:495	The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification.
26413620	2	59	theme	GO-LCO+	383:389	arg1	composition					368:378	composition	368:378	composition	368:378	The formation and composition of GO-LCO+ were confirmed by FTIR, AFM, TGA and zeta-potential.
26413620	2	59	theme	GO-LCO+	383:389	arg1	formation					354:362	formation	354:362	formation	354:362	The formation and composition of GO-LCO+ were confirmed by FTIR, AFM, TGA and zeta-potential.
26413620	1	60	theme	functionalized	247:260	arg1	GO-LCO					279:284	GO-LCO	279:284	GO-LCO	279:284	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	1	60	theme	functionalized	247:260	arg1	oxides					271:276	lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides	203:276	lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+)	203:333	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	4	61	theme	pH	991:992	arg1	dependence					994:1003	strong pH dependence	984:1003	strong pH dependence	984:1003	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	1	62	dep	ammonium	309:316	arg1	GO-LCO+					326:332	GO-LCO+	326:332	GO-LCO+	326:332	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	1	62	dep	ammonium	309:316	arg1	groups					318:323	groups	318:323	quaternary ammonium groups (GO-LCO+)	298:333	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	4	63	from	release	951:957	arg1	GO-LCO+					969:975	GO-LCO+	969:975	GO-LCO+	969:975	The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence.
26413620	1	64	dep	order	115:119	arg1	realize					124:130	realize	124:130	to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene	121:200	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	1	65	theme	graphene	262:269	arg1	GO-LCO					279:284	GO-LCO	279:284	GO-LCO	279:284	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26413620	1	65	theme	graphene	262:269	arg1	oxides					271:276	lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides	203:276	lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+)	203:333	In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared.
26920945	12	0	theme	pectin	1824:1829	arg1	degradation					1831:1841	pectin degradation	1824:1841	pectin degradation	1824:1841	The mutant showed strong growth reduction, thus confirming that PUL 49 plays a major role in pectin degradation.
26920945	4	1	theme	enzyme	525:530	arg1	systems					532:538	their enzyme systems	519:538	their enzyme systems	519:538	Likewise, little is known on gut pectinolytic bacteria and their enzyme systems.
26920945	5	2	theme	pectin	600:605	arg1	degradation					607:617	pectin degradation	600:617	pectin degradation	600:617	This study was undertaken to investigate the mechanisms of pectin degradation by the prominent human gut symbiont Bacteroides xylanisolvens.
26920945	6	3	theme	apple	759:763	arg1	pectins					765:771	citrus and apple pectins	748:771	citrus and apple pectins	748:771	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	1	4	theme	gut	178:180	arg1	microbiome					182:191	the gut microbiome	174:191	the gut microbiome	174:191	BACKGROUND Diet and particularly dietary fibres have an impact on the gut microbiome and play an important role in human health and disease.
26920945	6	5	theme	citrus	748:753	arg1	pectins					765:771	citrus and apple pectins	748:771	citrus and apple pectins	748:771	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	8	6	theme	CAZyme	1091:1096	arg1	composition					1098:1108	their CAZyme composition	1085:1108	their CAZyme composition	1085:1108	Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively.
26920945	16	7	theme	cell	2408:2411	arg1	polysaccharides					2418:2432	plant cell wall polysaccharides	2402:2432	plant cell wall polysaccharides	2402:2432	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	5	8	theme	Bacteroides	655:665	arg1	xylanisolvens					667:679	the prominent human gut symbiont Bacteroides xylanisolvens	622:679	the prominent human gut symbiont Bacteroides xylanisolvens	622:679	This study was undertaken to investigate the mechanisms of pectin degradation by the prominent human gut symbiont Bacteroides xylanisolvens.
26920945	12	9	theme	strong	1749:1754	arg1	reduction					1763:1771	strong growth reduction	1749:1771	strong growth reduction	1749:1771	The mutant showed strong growth reduction, thus confirming that PUL 49 plays a major role in pectin degradation.
26920945	15	10	theme	gut	2288:2290	arg1	ecosystem					2292:2300	the human gut ecosystem	2278:2300	the human gut ecosystem	2278:2300	Our findings also highlight the metabolic plasticity of B. xylanisolvens towards dietary fibres that contributes to its competitive fitness within the human gut ecosystem.
26920945	5	11	theme	prominent	626:634	arg1	xylanisolvens					667:679	the prominent human gut symbiont Bacteroides xylanisolvens	622:679	the prominent human gut symbiont Bacteroides xylanisolvens	622:679	This study was undertaken to investigate the mechanisms of pectin degradation by the prominent human gut symbiont Bacteroides xylanisolvens.
26920945	14	12	theme	pectin	2123:2128	arg1	complexity					2109:2118	the structural complexity	2094:2118	the structural complexity of pectin	2094:2128	Hence, this species deploys a very complex enzymatic machinery that probably reflects the structural complexity of pectin.
26920945	5	13	theme	gut	642:644	arg1	xylanisolvens					667:679	the prominent human gut symbiont Bacteroides xylanisolvens	622:679	the prominent human gut symbiont Bacteroides xylanisolvens	622:679	This study was undertaken to investigate the mechanisms of pectin degradation by the prominent human gut symbiont Bacteroides xylanisolvens.
26920945	13	14	theme	PULs	1893:1896	arg1	existence					1876:1884	the existence	1872:1884	the existence of six PULs devoted to pectin degradation by B. xylanisolvens	1872:1946	CONCLUSION This study shows the existence of six PULs devoted to pectin degradation by B. xylanisolvens, one of them being particularly important in this function.
26920945	1	15	from	impact	164:169	arg1	microbiome					182:191	the gut microbiome	174:191	the gut microbiome	174:191	BACKGROUND Diet and particularly dietary fibres have an impact on the gut microbiome and play an important role in human health and disease.
26920945	13	16	from	important	1980:1988	arg1	function					1998:2005	this function	1993:2005	this function	1993:2005	CONCLUSION This study shows the existence of six PULs devoted to pectin degradation by B. xylanisolvens, one of them being particularly important in this function.
26920945	7	17	theme	PUL	914:916	arg1	numbers					918:924	The PUL numbers	910:924	The PUL numbers used in this report	910:944	The PUL numbers used in this report are those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/.
26920945	7	17	theme	PUL	914:916	arg1	those					950:954	those	950:954	those	950:954	The PUL numbers used in this report are those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/.
26920945	9	18	theme	arabinose-containing	1371:1390	arg1	chains					1397:1402	arabinose-containing side chains	1371:1402	arabinose-containing side chains	1371:1402	PUL 13 and PUL 2 could be involved in the degradation of arabinose-containing side chains and of type II rhamnogalacturonan (RGII), respectively.
26920945	2	19	theme	food	364:367	arg1	industry					369:376	the food industry	360:376	the food industry	360:376	Pectin is a highly consumed dietary fibre found in fruits and vegetables and is also a widely used additive in the food industry.
26920945	9	20	theme	chains	1397:1402	arg1	degradation					1356:1366	the degradation	1352:1366	the degradation of arabinose-containing side chains and of type II rhamnogalacturonan (RGII)	1352:1443	PUL 13 and PUL 2 could be involved in the degradation of arabinose-containing side chains and of type II rhamnogalacturonan (RGII), respectively.
26920945	16	21	theme	gut	2529:2531	arg1	ecosystem					2543:2551	the gut microbial ecosystem	2525:2551	the gut microbial ecosystem	2525:2551	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	14	22	theme	complex	2043:2049	arg1	machinery					2061:2069	a very complex enzymatic machinery	2036:2069	a very complex enzymatic machinery that probably reflects the structural complexity of pectin	2036:2128	Hence, this species deploys a very complex enzymatic machinery that probably reflects the structural complexity of pectin.
26920945	11	23	theme	downstream	1695:1704	arg1	susD-like/FnIII					1713:1727	susD-like/FnIII	1713:1727	susD-like/FnIII	1713:1727	The insertion blocked transcription of the susC-like and the two downstream genes (susD-like/FnIII).
26920945	11	23	theme	downstream	1695:1704	arg1	genes					1706:1710	the two downstream genes	1687:1710	the two downstream genes (susD-like/FnIII)	1687:1728	The insertion blocked transcription of the susC-like and the two downstream genes (susD-like/FnIII).
26920945	4	24	theme	gut	489:491	arg1	bacteria					506:513	gut pectinolytic bacteria	489:513	gut pectinolytic bacteria	489:513	Likewise, little is known on gut pectinolytic bacteria and their enzyme systems.
26920945	0	25	theme	pectinolytic	15:26	arg1	function					28:35	the pectinolytic function	11:35	the pectinolytic function of Bacteroides xylanisolvens	11:64	Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis.
26920945	16	26	dep	composition	2444:2454	arg1	the					2440:2442	the	2440:2442	the	2440:2442	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	2	27	located	found	291:295	arg1	vegetables					311:320	vegetables	311:320	vegetables	311:320	Pectin is a highly consumed dietary fibre found in fruits and vegetables and is also a widely used additive in the food industry.
26920945	2	27	located	found	291:295	arg2	Pectin					249:254	Pectin	249:254	Pectin	249:254	Pectin is a highly consumed dietary fibre found in fruits and vegetables and is also a widely used additive in the food industry.
26920945	2	27	located	found	291:295	arg2	fibre					285:289	fibre	285:289	fibre	285:289	Pectin is a highly consumed dietary fibre found in fruits and vegetables and is also a widely used additive in the food industry.
26920945	2	27	located	found	291:295	arg1	fruits					300:305	fruits	300:305	fruits	300:305	Pectin is a highly consumed dietary fibre found in fruits and vegetables and is also a widely used additive in the food industry.
26920945	7	28	dep	those	950:954	arg1	found					1021:1025	found	1021:1025	found in the PUL database	1021:1045	The PUL numbers used in this report are those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/.
26920945	7	28	dep	those	950:954	arg1	//www.cazy.org/PULDB/					1053:1073	//www.cazy.org/PULDB/	1053:1073	those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/	950:1073	The PUL numbers used in this report are those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/.
26920945	7	28	dep	those	950:954	arg1	given					956:960	given	956:960	given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015)	956:1015	The PUL numbers used in this report are those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/.
26920945	7	28	dep	those	950:954	arg1	http					1048:1051	http	1048:1051	those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/	950:1073	The PUL numbers used in this report are those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/.
26920945	10	29	theme	abundant	1492:1499	arg1	moiety					1501:1506	the most abundant moiety	1483:1506	the most abundant moiety (>70%)	1483:1513	Considering that HG is the most abundant moiety (>70%) within pectin, the importance of PUL 49 was further investigated by insertion mutagenesis into the susC-like gene.
26920945	10	29	theme	abundant	1492:1499	arg1	%					1512:1512	>70%	1509:1512	>70%	1509:1512	Considering that HG is the most abundant moiety (>70%) within pectin, the importance of PUL 49 was further investigated by insertion mutagenesis into the susC-like gene.
26920945	10	29	theme	abundant	1492:1499	arg1	HG					1477:1478	HG	1477:1478	HG	1477:1478	Considering that HG is the most abundant moiety (>70%) within pectin, the importance of PUL 49 was further investigated by insertion mutagenesis into the susC-like gene.
26920945	6	30	theme	utilization	832:842	arg1	PUL					850:852	PUL	850:852	PUL	850:852	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	6	30	theme	utilization	832:842	arg1	loci					844:847	six polysaccharide utilization loci	813:847	six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose	813:907	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	8	31	theme	type	1255:1258	arg1	degradation					1287:1297	type I rhamnogalacturonan (RGI) degradation	1255:1297	type I rhamnogalacturonan (RGI) degradation	1255:1297	Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively.
26920945	3	32	from	effect	414:419	arg1	microbiome					448:457	the human gut microbiome	434:457	the human gut microbiome	434:457	Yet there is no information on the effect of pectin on the human gut microbiome.
26920945	3	33	theme	human	438:442	arg1	microbiome					448:457	the human gut microbiome	434:457	the human gut microbiome	434:457	Yet there is no information on the effect of pectin on the human gut microbiome.
26920945	7	34	theme	PUL	1034:1036	arg1	database					1038:1045	the PUL database	1030:1045	the PUL database	1030:1045	The PUL numbers used in this report are those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/.
26920945	8	35	theme	rhamnogalacturonan	1262:1279	arg1	degradation					1287:1297	type I rhamnogalacturonan (RGI) degradation	1255:1297	type I rhamnogalacturonan (RGI) degradation	1255:1297	Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively.
26920945	6	36	theme	B.	717:718	arg1	XB1A					734:737	B. xylanisolvens XB1A	717:737	B. xylanisolvens XB1A	717:737	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	16	37	theme	fibrolytic	2478:2487	arg1	community					2508:2516	the fibrolytic and non-fibrolytic community	2474:2516	the fibrolytic and non-fibrolytic community	2474:2516	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	8	38	theme	RGI	1282:1284	arg1	degradation					1287:1297	type I rhamnogalacturonan (RGI) degradation	1255:1297	type I rhamnogalacturonan (RGI) degradation	1255:1297	Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively.
26920945	3	39	from	information	395:405	arg1	effect					414:419	the effect	410:419	the effect of pectin on the human gut microbiome	410:457	Yet there is no information on the effect of pectin on the human gut microbiome.
26920945	12	40	theme	major	1810:1814	arg1	role					1816:1819	a major role	1808:1819	a major role	1808:1819	The mutant showed strong growth reduction, thus confirming that PUL 49 plays a major role in pectin degradation.
26920945	6	41	theme	relative	889:896	arg1	pectin					882:887	pectin	882:887	pectin relative to glucose	882:907	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	16	42	theme	non-fibrolytic	2493:2506	arg1	community					2508:2516	the fibrolytic and non-fibrolytic community	2474:2516	the fibrolytic and non-fibrolytic community	2474:2516	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	11	43	theme	susC-like	1673:1681	arg1	susD-like/FnIII					1713:1727	susD-like/FnIII	1713:1727	susD-like/FnIII	1713:1727	The insertion blocked transcription of the susC-like and the two downstream genes (susD-like/FnIII).
26920945	11	43	theme	susC-like	1673:1681	arg1	transcription					1652:1664	transcription	1652:1664	transcription of the susC-like	1652:1681	The insertion blocked transcription of the susC-like and the two downstream genes (susD-like/FnIII).
26920945	11	43	theme	susC-like	1673:1681	arg1	genes					1706:1710	the two downstream genes	1687:1710	the two downstream genes (susD-like/FnIII)	1687:1728	The insertion blocked transcription of the susC-like and the two downstream genes (susD-like/FnIII).
26920945	16	44	theme	functional	2309:2318	arg1	studies					2335:2341	Wider functional and ecological studies	2303:2341	Wider functional and ecological studies	2303:2341	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	5	45	theme	degradation	607:617	arg1	mechanisms					586:595	the mechanisms	582:595	the mechanisms of pectin degradation by the prominent human gut symbiont Bacteroides xylanisolvens	582:679	This study was undertaken to investigate the mechanisms of pectin degradation by the prominent human gut symbiont Bacteroides xylanisolvens.
26920945	6	46	theme	mid-	776:779	arg1	phases					794:799	mid- and late-log phases	776:799	mid- and late-log phases	776:799	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	6	47	theme	XB1A	734:737	arg1	analyses					705:712	Transcriptomic analyses	690:712	Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases	690:799	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	10	48	theme	PUL	1548:1550	arg1	importance					1534:1543	the importance	1530:1543	the importance of PUL 49	1530:1553	Considering that HG is the most abundant moiety (>70%) within pectin, the importance of PUL 49 was further investigated by insertion mutagenesis into the susC-like gene.
26920945	0	49	theme	xylanisolvens	52:64	arg1	function					28:35	the pectinolytic function	11:35	the pectinolytic function of Bacteroides xylanisolvens	11:64	Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis.
26920945	1	50	contain	have	156:159	arg2	impact					164:169	an impact	161:169	an impact on the gut microbiome	161:191	BACKGROUND Diet and particularly dietary fibres have an impact on the gut microbiome and play an important role in human health and disease.
26920945	1	50	contain	have	156:159	arg1	fibres					149:154	dietary fibres	141:154	dietary fibres	141:154	BACKGROUND Diet and particularly dietary fibres have an impact on the gut microbiome and play an important role in human health and disease.
26920945	1	50	contain	have	156:159	arg1	Diet					119:122	BACKGROUND Diet	108:122	BACKGROUND Diet	108:122	BACKGROUND Diet and particularly dietary fibres have an impact on the gut microbiome and play an important role in human health and disease.
26920945	10	51	theme	insertion	1583:1591	arg1	mutagenesis					1593:1603	insertion mutagenesis	1583:1603	insertion mutagenesis into the susC-like gene	1583:1627	Considering that HG is the most abundant moiety (>70%) within pectin, the importance of PUL 49 was further investigated by insertion mutagenesis into the susC-like gene.
26920945	16	52	theme	wall	2413:2416	arg1	polysaccharides					2418:2432	plant cell wall polysaccharides	2402:2432	plant cell wall polysaccharides	2402:2432	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	5	53	theme	symbiont	646:653	arg1	xylanisolvens					667:679	the prominent human gut symbiont Bacteroides xylanisolvens	622:679	the prominent human gut symbiont Bacteroides xylanisolvens	622:679	This study was undertaken to investigate the mechanisms of pectin degradation by the prominent human gut symbiont Bacteroides xylanisolvens.
26920945	13	54	theme	pectin	1909:1914	arg1	degradation					1916:1926	pectin degradation	1909:1926	pectin degradation	1909:1926	CONCLUSION This study shows the existence of six PULs devoted to pectin degradation by B. xylanisolvens, one of them being particularly important in this function.
26920945	10	55	theme	susC-like	1614:1622	arg1	gene					1624:1627	the susC-like gene	1610:1627	the susC-like gene	1610:1627	Considering that HG is the most abundant moiety (>70%) within pectin, the importance of PUL 49 was further investigated by insertion mutagenesis into the susC-like gene.
26920945	1	56	theme	important	205:213	arg1	role					215:218	an important role	202:218	an important role	202:218	BACKGROUND Diet and particularly dietary fibres have an impact on the gut microbiome and play an important role in human health and disease.
26920945	15	57	theme	metabolic	2163:2171	arg1	plasticity					2173:2182	the metabolic plasticity	2159:2182	the metabolic plasticity of B. xylanisolvens towards dietary fibres that contributes to its competitive fitness within the human gut ecosystem	2159:2300	Our findings also highlight the metabolic plasticity of B. xylanisolvens towards dietary fibres that contributes to its competitive fitness within the human gut ecosystem.
26920945	12	58	theme	growth	1756:1761	arg1	reduction					1763:1771	strong growth reduction	1749:1771	strong growth reduction	1749:1771	The mutant showed strong growth reduction, thus confirming that PUL 49 plays a major role in pectin degradation.
26920945	8	59	theme	overexpressed	1151:1163	arg1	PULs					1165:1168	the most overexpressed PULs	1142:1168	the most overexpressed PULs on both pectins and at both growth phases	1142:1210	Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively.
26920945	8	59	theme	overexpressed	1151:1163	arg1	PUL					1127:1129	PUL	1127:1129	PUL	1127:1129	Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively.
26920945	5	60	theme	human	636:640	arg1	xylanisolvens					667:679	the prominent human gut symbiont Bacteroides xylanisolvens	622:679	the prominent human gut symbiont Bacteroides xylanisolvens	622:679	This study was undertaken to investigate the mechanisms of pectin degradation by the prominent human gut symbiont Bacteroides xylanisolvens.
26920945	16	61	dep	functional	2309:2318	arg1	Wider					2303:2307	Wider	2303:2307	Wider	2303:2307	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	15	62	theme	human	2282:2286	arg1	ecosystem					2292:2300	the human gut ecosystem	2278:2300	the human gut ecosystem	2278:2300	Our findings also highlight the metabolic plasticity of B. xylanisolvens towards dietary fibres that contributes to its competitive fitness within the human gut ecosystem.
26920945	1	63	theme	dietary	141:147	arg1	fibres					149:154	dietary fibres	141:154	dietary fibres	141:154	BACKGROUND Diet and particularly dietary fibres have an impact on the gut microbiome and play an important role in human health and disease.
26920945	0	64	theme	RNA-seq	74:80	arg1	approach					82:89	a RNA-seq approach	72:89	a RNA-seq approach	72:89	Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis.
26920945	16	65	theme	dietary	2372:2378	arg1	fibers					2380:2385	dietary fibers	2372:2385	dietary fibers	2372:2385	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	9	66	theme	type	1411:1414	arg1	RGII					1439:1442	RGII	1439:1442	RGII	1439:1442	PUL 13 and PUL 2 could be involved in the degradation of arabinose-containing side chains and of type II rhamnogalacturonan (RGII), respectively.
26920945	9	66	theme	type	1411:1414	arg1	rhamnogalacturonan					1419:1436	type II rhamnogalacturonan	1411:1436	type II rhamnogalacturonan (RGII)	1411:1443	PUL 13 and PUL 2 could be involved in the degradation of arabinose-containing side chains and of type II rhamnogalacturonan (RGII), respectively.
26920945	14	67	theme	structural	2098:2107	arg1	complexity					2109:2118	the structural complexity	2094:2118	the structural complexity of pectin	2094:2128	Hence, this species deploys a very complex enzymatic machinery that probably reflects the structural complexity of pectin.
26920945	15	68	theme	competitive	2251:2261	arg1	fitness					2263:2269	its competitive fitness	2247:2269	its competitive fitness within the human gut ecosystem	2247:2300	Our findings also highlight the metabolic plasticity of B. xylanisolvens towards dietary fibres that contributes to its competitive fitness within the human gut ecosystem.
26920945	13	69	from	function	1998:2005	arg1	important					1980:1988	important	1980:1988	important	1980:1988	CONCLUSION This study shows the existence of six PULs devoted to pectin degradation by B. xylanisolvens, one of them being particularly important in this function.
26920945	8	70	from	PULs	1165:1168	arg1	pectins					1178:1184	both pectins	1173:1184	both pectins	1173:1184	Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively.
26920945	16	71	theme	plant	2402:2406	arg1	polysaccharides					2418:2432	plant cell wall polysaccharides	2402:2432	plant cell wall polysaccharides	2402:2432	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	8	72	theme	growth	1198:1203	arg1	phases					1205:1210	both growth phases	1193:1210	both growth phases	1193:1210	Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively.
26920945	15	73	theme	dietary	2212:2218	arg1	fibres					2220:2225	dietary fibres	2212:2225	dietary fibres that contributes to its competitive fitness within the human gut ecosystem	2212:2300	Our findings also highlight the metabolic plasticity of B. xylanisolvens towards dietary fibres that contributes to its competitive fitness within the human gut ecosystem.
26920945	9	74	theme	side	1392:1395	arg1	chains					1397:1402	arabinose-containing side chains	1371:1402	arabinose-containing side chains	1371:1402	PUL 13 and PUL 2 could be involved in the degradation of arabinose-containing side chains and of type II rhamnogalacturonan (RGII), respectively.
26920945	16	75	theme	microbial	2533:2541	arg1	ecosystem					2543:2551	the gut microbial ecosystem	2525:2551	the gut microbial ecosystem	2525:2551	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	1	76	theme	human	223:227	arg1	health					229:234	human health	223:234	human health	223:234	BACKGROUND Diet and particularly dietary fibres have an impact on the gut microbiome and play an important role in human health and disease.
26920945	7	77	dep	Terrapon	965:972	arg1	al					977:978	Terrapon et al	965:978	Terrapon et al. (Bioinformatics 31(5):647-55, 2015)	965:1015	The PUL numbers used in this report are those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/.
26920945	7	77	dep	Terrapon	965:972	arg1	Bioinformatics					982:995	Bioinformatics	982:995	Bioinformatics	982:995	The PUL numbers used in this report are those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/.
26920945	15	78	theme	xylanisolvens	2190:2202	arg1	plasticity					2173:2182	the metabolic plasticity	2159:2182	the metabolic plasticity of B. xylanisolvens towards dietary fibres that contributes to its competitive fitness within the human gut ecosystem	2159:2300	Our findings also highlight the metabolic plasticity of B. xylanisolvens towards dietary fibres that contributes to its competitive fitness within the human gut ecosystem.
26920945	4	79	theme	pectinolytic	493:504	arg1	bacteria					506:513	gut pectinolytic bacteria	489:513	gut pectinolytic bacteria	489:513	Likewise, little is known on gut pectinolytic bacteria and their enzyme systems.
26920945	1	80	theme	BACKGROUND	108:117	arg1	Diet					119:122	BACKGROUND Diet	108:122	BACKGROUND Diet	108:122	BACKGROUND Diet and particularly dietary fibres have an impact on the gut microbiome and play an important role in human health and disease.
26920945	3	81	theme	pectin	424:429	arg1	effect					414:419	the effect	410:419	the effect of pectin on the human gut microbiome	410:457	Yet there is no information on the effect of pectin on the human gut microbiome.
26920945	0	82	theme	Bacteroides	40:50	arg1	xylanisolvens					52:64	Bacteroides xylanisolvens	40:64	Bacteroides xylanisolvens	40:64	Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis.
26920945	9	83	theme	rhamnogalacturonan	1419:1436	arg1	degradation					1356:1366	the degradation	1352:1366	the degradation of arabinose-containing side chains and of type II rhamnogalacturonan (RGII)	1352:1443	PUL 13 and PUL 2 could be involved in the degradation of arabinose-containing side chains and of type II rhamnogalacturonan (RGII), respectively.
26920945	13	84	dep	CONCLUSION	1844:1853	arg1	shows					1866:1870	shows	1866:1870	shows	1866:1870	CONCLUSION This study shows the existence of six PULs devoted to pectin degradation by B. xylanisolvens, one of them being particularly important in this function.
26920945	6	85	theme	polysaccharide	817:830	arg1	PUL					850:852	PUL	850:852	PUL	850:852	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	6	85	theme	polysaccharide	817:830	arg1	loci					844:847	six polysaccharide utilization loci	813:847	six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose	813:907	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	2	86	from	additive	348:355	arg1	industry					369:376	the food industry	360:376	the food industry	360:376	Pectin is a highly consumed dietary fibre found in fruits and vegetables and is also a widely used additive in the food industry.
26920945	6	87	theme	xylanisolvens	720:732	arg1	XB1A					734:737	B. xylanisolvens XB1A	717:737	B. xylanisolvens XB1A	717:737	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	3	88	theme	gut	444:446	arg1	microbiome					448:457	the human gut microbiome	434:457	the human gut microbiome	434:457	Yet there is no information on the effect of pectin on the human gut microbiome.
26920945	14	89	theme	enzymatic	2051:2059	arg1	machinery					2061:2069	a very complex enzymatic machinery	2036:2069	a very complex enzymatic machinery that probably reflects the structural complexity of pectin	2036:2128	Hence, this species deploys a very complex enzymatic machinery that probably reflects the structural complexity of pectin.
26920945	16	90	theme	ecological	2324:2333	arg1	studies					2335:2341	Wider functional and ecological studies	2303:2341	Wider functional and ecological studies	2303:2341	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	6	91	dep	RESULTS	682:688	arg1	highlighted					801:811	highlighted	801:811	highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose	801:907	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	8	92	theme	I	1260:1260	arg1	degradation					1287:1297	type I rhamnogalacturonan (RGI) degradation	1255:1297	type I rhamnogalacturonan (RGI) degradation	1255:1297	Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively.
26920945	8	93	from	phases	1205:1210	arg1	PULs					1165:1168	the most overexpressed PULs	1142:1168	the most overexpressed PULs on both pectins and at both growth phases	1142:1210	Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively.
26920945	8	93	from	phases	1205:1210	arg1	PUL					1127:1129	PUL	1127:1129	PUL	1127:1129	Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively.
26920945	13	94	theme	them	1956:1959	arg1	them					1956:1959	them	1956:1959	them	1956:1959	CONCLUSION This study shows the existence of six PULs devoted to pectin degradation by B. xylanisolvens, one of them being particularly important in this function.
26920945	13	94	theme	them	1956:1959	arg1	one					1949:1951	one	1949:1951	one	1949:1951	CONCLUSION This study shows the existence of six PULs devoted to pectin degradation by B. xylanisolvens, one of them being particularly important in this function.
26920945	6	95	theme	late-log	785:792	arg1	phases					794:799	mid- and late-log phases	776:799	mid- and late-log phases	776:799	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
26920945	2	96	from	industry	369:376	arg1	additive					348:355	additive	348:355	additive	348:355	Pectin is a highly consumed dietary fibre found in fruits and vegetables and is also a widely used additive in the food industry.
26920945	2	96	from	industry	369:376	arg1	Pectin					249:254	Pectin	249:254	Pectin	249:254	Pectin is a highly consumed dietary fibre found in fruits and vegetables and is also a widely used additive in the food industry.
26920945	16	97	theme	community	2508:2516	arg1	composition					2444:2454	composition	2444:2454	composition	2444:2454	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	16	97	theme	community	2508:2516	arg1	metabolism					2460:2469	metabolism	2460:2469	metabolism	2460:2469	Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.
26920945	6	98	theme	Transcriptomic	690:703	arg1	analyses					705:712	Transcriptomic analyses	690:712	Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases	690:799	RESULTS Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose.
28262019	2	0	dep	15	460:461	arg1	ca.					456:458	ca.	456:458	ca.	456:458	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	3	1	theme	wet	496:498	arg1	filaments					500:508	the as-formed wet filaments	482:508	the as-formed wet filaments of TOCN/polycation	482:527	Stretching of the as-formed wet filaments of TOCN/polycation by 20% increased the Young's modulus, yield strength, and ultimate tensile strength by approximately 45, 36, and 26%, respectively.
28262019	2	2	theme	ultimate	382:389	arg1	strengths					391:399	ultimate strengths	382:399	ultimate strengths of ca. 100 MPa and ca. 200 MPa	382:430	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	6	3	from	crimping	1213:1220	arg1	application					1152:1162	the application	1148:1162	the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles	1148:1251	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	6	4	from	textiles	1244:1251	arg1	application					1152:1162	the application	1148:1162	the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles	1148:1251	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	2	5	dep	100	408:410	arg1	ca.					404:406	ca.	404:406	ca.	404:406	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	4	6	theme	simultaneous	735:746	arg1	spinning					748:755	simultaneous spinning	735:755	simultaneous spinning of two fibers of different compositions	735:795	Importantly, feasibility of compartmentalized wound bicomponent fibers by simultaneous spinning of two fibers of different compositions and entwining them together was shown.
28262019	3	7	theme	Young	550:554	arg1	modulus					558:564	the Young's modulus	546:564	the Young's modulus	546:564	Stretching of the as-formed wet filaments of TOCN/polycation by 20% increased the Young's modulus, yield strength, and ultimate tensile strength by approximately 45, 36, and 26%, respectively.
28262019	4	8	theme	wound	707:711	arg1	fibers					725:730	compartmentalized wound bicomponent fibers	689:730	compartmentalized wound bicomponent fibers	689:730	Importantly, feasibility of compartmentalized wound bicomponent fibers by simultaneous spinning of two fibers of different compositions and entwining them together was shown.
28262019	1	9	with	nanofibrils	161:171	arg1	polycations					178:188	polycations	178:188	polycations	178:188	Fiber spinning of anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations by interfacial polyelectrolyte complexation is demonstrated.
28262019	6	10	from	nanocelluloses	1167:1180	arg1	textiles					1244:1251	responsive smart textiles	1227:1251	responsive smart textiles	1227:1251	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	6	10	from	nanocelluloses	1167:1180	arg1	crimping					1213:1220	crimping	1213:1220	crimping	1213:1220	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	6	10	from	nanocelluloses	1167:1180	arg1	materials					1202:1210	advanced fibrous materials	1185:1210	advanced fibrous materials	1185:1210	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	5	11	theme	shape	901:905	arg1	change					907:912	reversible shape change	890:912	reversible shape change of a bicomponent fiber	890:935	This possibility was further exploited to demonstrate reversible shape change of a bicomponent fiber directly by humidity change, and indirectly by temperature changes based on thermally dependent humidity absorption.
28262019	6	12	theme	nanocelluloses	1167:1180	arg1	application					1152:1162	the application	1148:1162	the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles	1148:1251	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	2	13	theme	MPa	412:414	arg1	modulus					445:451	Young's modulus	437:451	Young's modulus of ca. 15 GPa	437:465	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	2	13	theme	MPa	412:414	arg1	strengths					391:399	ultimate strengths	382:399	ultimate strengths of ca. 100 MPa and ca. 200 MPa	382:430	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	6	14	theme	TOCN-based	1081:1090	arg1	preparation					1098:1108	TOCN-based fiber preparation	1081:1108	TOCN-based fiber preparation	1081:1108	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	5	15	theme	reversible	890:899	arg1	change					907:912	reversible shape change	890:912	reversible shape change of a bicomponent fiber	890:935	This possibility was further exploited to demonstrate reversible shape change of a bicomponent fiber directly by humidity change, and indirectly by temperature changes based on thermally dependent humidity absorption.
28262019	5	16	theme	bicomponent	919:929	arg1	fiber					931:935	a bicomponent fiber	917:935	a bicomponent fiber	917:935	This possibility was further exploited to demonstrate reversible shape change of a bicomponent fiber directly by humidity change, and indirectly by temperature changes based on thermally dependent humidity absorption.
28262019	6	17	theme	fiber	1092:1096	arg1	preparation					1098:1108	TOCN-based fiber preparation	1081:1108	TOCN-based fiber preparation	1081:1108	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	1	18	theme	interfacial	193:203	arg1	complexation					221:232	interfacial polyelectrolyte complexation	193:232	interfacial polyelectrolyte complexation	193:232	Fiber spinning of anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations by interfacial polyelectrolyte complexation is demonstrated.
28262019	0	19	theme	Polyelectrolyte	12:26	arg1	Spinning					36:43	Interfacial Polyelectrolyte Complex Spinning	0:43	Interfacial Polyelectrolyte Complex Spinning of Cellulose Nanofibrils for Advanced Bicomponent Fibers.	0:101	Interfacial Polyelectrolyte Complex Spinning of Cellulose Nanofibrils for Advanced Bicomponent Fibers.
28262019	3	20	theme	TOCN/polycation	513:527	arg1	filaments					500:508	the as-formed wet filaments	482:508	the as-formed wet filaments of TOCN/polycation	482:527	Stretching of the as-formed wet filaments of TOCN/polycation by 20% increased the Young's modulus, yield strength, and ultimate tensile strength by approximately 45, 36, and 26%, respectively.
28262019	1	21	theme	Fiber	103:107	arg1	spinning					109:116	Fiber spinning	103:116	Fiber spinning of anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations by interfacial polyelectrolyte complexation	103:232	Fiber spinning of anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations by interfacial polyelectrolyte complexation is demonstrated.
28262019	1	22	theme	polyelectrolyte	205:219	arg1	complexation					221:232	interfacial polyelectrolyte complexation	193:232	interfacial polyelectrolyte complexation	193:232	Fiber spinning of anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations by interfacial polyelectrolyte complexation is demonstrated.
28262019	0	23	theme	Interfacial	0:10	arg1	Spinning					36:43	Interfacial Polyelectrolyte Complex Spinning	0:43	Interfacial Polyelectrolyte Complex Spinning of Cellulose Nanofibrils for Advanced Bicomponent Fibers.	0:101	Interfacial Polyelectrolyte Complex Spinning of Cellulose Nanofibrils for Advanced Bicomponent Fibers.
28262019	4	24	theme	compartmentalized	689:705	arg1	fibers					725:730	compartmentalized wound bicomponent fibers	689:730	compartmentalized wound bicomponent fibers	689:730	Importantly, feasibility of compartmentalized wound bicomponent fibers by simultaneous spinning of two fibers of different compositions and entwining them together was shown.
28262019	6	25	theme	demonstrated	1058:1069	arg1	route					1071:1075	The demonstrated route	1054:1075	The demonstrated route for TOCN-based fiber preparation	1054:1108	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	5	26	theme	temperature	984:994	arg1	changes					996:1002	temperature changes	984:1002	temperature changes based on thermally dependent humidity absorption	984:1051	This possibility was further exploited to demonstrate reversible shape change of a bicomponent fiber directly by humidity change, and indirectly by temperature changes based on thermally dependent humidity absorption.
28262019	3	27	theme	yield	567:571	arg1	strength					573:580	yield strength	567:580	yield strength	567:580	Stretching of the as-formed wet filaments of TOCN/polycation by 20% increased the Young's modulus, yield strength, and ultimate tensile strength by approximately 45, 36, and 26%, respectively.
28262019	2	28	theme	minor	342:346	arg1	polycations					323:333	the polycations	319:333	the polycations	319:333	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	2	28	theme	minor	342:346	arg1	constituent					348:358	a minor constituent	340:358	a minor constituent	340:358	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	5	29	theme	humidity	949:956	arg1	change					958:963	humidity change	949:963	humidity change	949:963	This possibility was further exploited to demonstrate reversible shape change of a bicomponent fiber directly by humidity change, and indirectly by temperature changes based on thermally dependent humidity absorption.
28262019	0	30	theme	Complex	28:34	arg1	Spinning					36:43	Interfacial Polyelectrolyte Complex Spinning	0:43	Interfacial Polyelectrolyte Complex Spinning of Cellulose Nanofibrils for Advanced Bicomponent Fibers.	0:101	Interfacial Polyelectrolyte Complex Spinning of Cellulose Nanofibrils for Advanced Bicomponent Fibers.
28262019	5	31	theme	fiber	931:935	arg1	change					907:912	reversible shape change	890:912	reversible shape change of a bicomponent fiber	890:935	This possibility was further exploited to demonstrate reversible shape change of a bicomponent fiber directly by humidity change, and indirectly by temperature changes based on thermally dependent humidity absorption.
28262019	6	32	theme	advanced	1185:1192	arg1	materials					1202:1210	advanced fibrous materials	1185:1210	advanced fibrous materials	1185:1210	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	1	33	theme	anionic	121:127	arg1	nanofibrils					161:171	anionic TEMPO-oxidized cellulose (TOCN) nanofibrils	121:171	anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations	121:188	Fiber spinning of anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations by interfacial polyelectrolyte complexation is demonstrated.
28262019	6	34	from	application	1152:1162	arg1	textiles					1244:1251	responsive smart textiles	1227:1251	responsive smart textiles	1227:1251	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	6	34	from	application	1152:1162	arg1	crimping					1213:1220	crimping	1213:1220	crimping	1213:1220	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	6	34	from	application	1152:1162	arg1	materials					1202:1210	advanced fibrous materials	1185:1210	advanced fibrous materials	1185:1210	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	4	35	dep	entwining	801:809	arg1	them					811:814	them	811:814	them	811:814	Importantly, feasibility of compartmentalized wound bicomponent fibers by simultaneous spinning of two fibers of different compositions and entwining them together was shown.
28262019	0	36	theme	Cellulose	48:56	arg1	Nanofibrils					58:68	Cellulose Nanofibrils	48:68	Cellulose Nanofibrils	48:68	Interfacial Polyelectrolyte Complex Spinning of Cellulose Nanofibrils for Advanced Bicomponent Fibers.
28262019	3	37	theme	as-formed	486:494	arg1	filaments					500:508	the as-formed wet filaments	482:508	the as-formed wet filaments of TOCN/polycation	482:527	Stretching of the as-formed wet filaments of TOCN/polycation by 20% increased the Young's modulus, yield strength, and ultimate tensile strength by approximately 45, 36, and 26%, respectively.
28262019	6	38	theme	smart	1238:1242	arg1	textiles					1244:1251	responsive smart textiles	1227:1251	responsive smart textiles	1227:1251	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	6	39	theme	fibrous	1194:1200	arg1	materials					1202:1210	advanced fibrous materials	1185:1210	advanced fibrous materials	1185:1210	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	2	40	theme	MPa	428:430	arg1	modulus					445:451	Young's modulus	437:451	Young's modulus of ca. 15 GPa	437:465	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	2	40	theme	MPa	428:430	arg1	strengths					391:399	ultimate strengths	382:399	ultimate strengths of ca. 100 MPa and ca. 200 MPa	382:430	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	2	41	theme	GPa	463:465	arg1	modulus					445:451	Young's modulus	437:451	Young's modulus of ca. 15 GPa	437:465	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	2	41	theme	GPa	463:465	arg1	strengths					391:399	ultimate strengths	382:399	ultimate strengths of ca. 100 MPa and ca. 200 MPa	382:430	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	2	42	dep	200	424:426	arg1	ca.					420:422	ca.	420:422	ca.	420:422	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	1	43	theme	TEMPO-oxidized	129:142	arg1	nanofibrils					161:171	anionic TEMPO-oxidized cellulose (TOCN) nanofibrils	121:171	anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations	121:188	Fiber spinning of anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations by interfacial polyelectrolyte complexation is demonstrated.
28262019	0	44	theme	Nanofibrils	58:68	arg1	Spinning					36:43	Interfacial Polyelectrolyte Complex Spinning	0:43	Interfacial Polyelectrolyte Complex Spinning of Cellulose Nanofibrils for Advanced Bicomponent Fibers.	0:101	Interfacial Polyelectrolyte Complex Spinning of Cellulose Nanofibrils for Advanced Bicomponent Fibers.
28262019	6	45	theme	responsive	1227:1236	arg1	textiles					1244:1251	responsive smart textiles	1227:1251	responsive smart textiles	1227:1251	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	5	46	theme	humidity	1033:1040	arg1	absorption					1042:1051	thermally dependent humidity absorption	1013:1051	thermally dependent humidity absorption	1013:1051	This possibility was further exploited to demonstrate reversible shape change of a bicomponent fiber directly by humidity change, and indirectly by temperature changes based on thermally dependent humidity absorption.
28262019	2	47	theme	formed	255:260	arg1	fibers					262:267	The formed fibers	251:267	The formed fibers	251:267	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	3	48	theme	filaments	500:508	arg1	Stretching					468:477	Stretching	468:477	Stretching of the as-formed wet filaments of TOCN/polycation by 20%	468:534	Stretching of the as-formed wet filaments of TOCN/polycation by 20% increased the Young's modulus, yield strength, and ultimate tensile strength by approximately 45, 36, and 26%, respectively.
28262019	1	49	theme	cellulose	144:152	arg1	nanofibrils					161:171	anionic TEMPO-oxidized cellulose (TOCN) nanofibrils	121:171	anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations	121:188	Fiber spinning of anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations by interfacial polyelectrolyte complexation is demonstrated.
28262019	3	50	theme	ultimate	587:594	arg1	strength					604:611	ultimate tensile strength	587:611	ultimate tensile strength	587:611	Stretching of the as-formed wet filaments of TOCN/polycation by 20% increased the Young's modulus, yield strength, and ultimate tensile strength by approximately 45, 36, and 26%, respectively.
28262019	4	51	theme	fibers	764:769	arg1	spinning					748:755	simultaneous spinning	735:755	simultaneous spinning of two fibers of different compositions	735:795	Importantly, feasibility of compartmentalized wound bicomponent fibers by simultaneous spinning of two fibers of different compositions and entwining them together was shown.
28262019	3	52	theme	tensile	596:602	arg1	strength					604:611	ultimate tensile strength	587:611	ultimate tensile strength	587:611	Stretching of the as-formed wet filaments of TOCN/polycation by 20% increased the Young's modulus, yield strength, and ultimate tensile strength by approximately 45, 36, and 26%, respectively.
28262019	4	53	theme	compositions	784:795	arg1	fibers					764:769	two fibers	760:769	two fibers of different compositions	760:795	Importantly, feasibility of compartmentalized wound bicomponent fibers by simultaneous spinning of two fibers of different compositions and entwining them together was shown.
28262019	4	54	theme	fibers	725:730	arg1	entwining					801:809	entwining	801:809	entwining them	801:814	Importantly, feasibility of compartmentalized wound bicomponent fibers by simultaneous spinning of two fibers of different compositions and entwining them together was shown.
28262019	4	54	theme	fibers	725:730	arg1	feasibility					674:684	feasibility	674:684	feasibility of compartmentalized wound bicomponent fibers by simultaneous spinning of two fibers of different compositions	674:795	Importantly, feasibility of compartmentalized wound bicomponent fibers by simultaneous spinning of two fibers of different compositions and entwining them together was shown.
28262019	2	55	theme	cellulose	293:301	arg1	nanofibrils					303:313	cellulose nanofibrils	293:313	cellulose nanofibrils	293:313	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	4	56	theme	bicomponent	713:723	arg1	fibers					725:730	compartmentalized wound bicomponent fibers	689:730	compartmentalized wound bicomponent fibers	689:730	Importantly, feasibility of compartmentalized wound bicomponent fibers by simultaneous spinning of two fibers of different compositions and entwining them together was shown.
28262019	1	57	theme	TOCN	155:158	arg1	nanofibrils					161:171	anionic TEMPO-oxidized cellulose (TOCN) nanofibrils	121:171	anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations	121:188	Fiber spinning of anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations by interfacial polyelectrolyte complexation is demonstrated.
28262019	6	58	theme	new	1133:1135	arg1	avenues					1137:1143	new avenues	1133:1143	new avenues	1133:1143	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
28262019	5	59	theme	dependent	1023:1031	arg1	absorption					1042:1051	thermally dependent humidity absorption	1013:1051	thermally dependent humidity absorption	1013:1051	This possibility was further exploited to demonstrate reversible shape change of a bicomponent fiber directly by humidity change, and indirectly by temperature changes based on thermally dependent humidity absorption.
28262019	0	60	theme	Bicomponent	83:93	arg1	Fibers					95:100	Advanced Bicomponent Fibers	74:100	Advanced Bicomponent Fibers	74:100	Interfacial Polyelectrolyte Complex Spinning of Cellulose Nanofibrils for Advanced Bicomponent Fibers.
28262019	4	61	theme	different	774:782	arg1	compositions					784:795	different compositions	774:795	different compositions	774:795	Importantly, feasibility of compartmentalized wound bicomponent fibers by simultaneous spinning of two fibers of different compositions and entwining them together was shown.
28262019	0	62	theme	Advanced	74:81	arg1	Fibers					95:100	Advanced Bicomponent Fibers	74:100	Advanced Bicomponent Fibers	74:100	Interfacial Polyelectrolyte Complex Spinning of Cellulose Nanofibrils for Advanced Bicomponent Fibers.
28262019	2	63	theme	Young	437:441	arg1	modulus					445:451	Young's modulus	437:451	Young's modulus of ca. 15 GPa	437:465	The formed fibers were mostly composed of cellulose nanofibrils and the polycations were a minor constituent, leading to yield and ultimate strengths of ca. 100 MPa and ca. 200 MPa, and Young's modulus of ca. 15 GPa.
28262019	1	64	theme	nanofibrils	161:171	arg1	spinning					109:116	Fiber spinning	103:116	Fiber spinning of anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations by interfacial polyelectrolyte complexation	103:232	Fiber spinning of anionic TEMPO-oxidized cellulose (TOCN) nanofibrils with polycations by interfacial polyelectrolyte complexation is demonstrated.
28262019	6	65	from	materials	1202:1210	arg1	application					1152:1162	the application	1148:1162	the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles	1148:1251	The demonstrated route for TOCN-based fiber preparation is expected to open up new avenues in the application of nanocelluloses in advanced fibrous materials, crimping, and responsive smart textiles.
27513732	6	0	theme	perennial	1243:1251	arg1	growth					1259:1264	supply perennial organ growth	1236:1264	supply perennial organ growth in both species	1236:1280	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	1	1	theme	short-term	323:332	arg1	dynamics					334:341	seasonal and interspecific short-term dynamics	296:341	seasonal and interspecific short-term dynamics	296:341	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	1	2	theme	assimilated	372:382	arg1	C					392:392	C	392:392	C	392:392	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	1	2	theme	assimilated	372:382	arg1	carbon					384:389	recently assimilated carbon	363:389	recently assimilated carbon (C)	363:393	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	1	3	theme	pulse-labelling	141:155	arg1	experiments					157:167	13 CO2 pulse-labelling experiments	134:167	13 CO2 pulse-labelling experiments	134:167	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	4	4	from	beech	869:873	arg1	May					885:887	May	885:887	May	885:887	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	4	4	from	beech	869:873	arg1	maximal					858:864	maximal	858:864	maximal	858:864	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	4	4	from	beech	869:873	arg1	proportion					827:836	the relative proportion	814:836	the relative proportion of the latter	814:850	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	2	5	theme	amino	452:456	arg1	acids					458:462	amino acids	452:462	amino acids	452:462	Polar fraction (PF, including soluble sugars, amino acids and organic acids) and starch were purified from foliage sampled during a 10-d chase period.
27513732	3	6	theme	bulk	639:642	arg1	foliage					644:650	bulk foliage	639:650	bulk foliage	639:650	C contents, isotopic compositions and 13 C dynamics parameters were determined in bulk foliage, PF and starch.
27513732	5	7	theme	C	949:949	arg1	sink					951:954	a strong C sink	940:954	a strong C sink in both species	940:970	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	5	7	theme	C	949:949	arg1	foliage					923:929	new foliage	919:929	new foliage	919:929	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	4	8	theme	stable	799:804	arg1	pools					807:811	'mobile' and 'stable' pools	785:811	'mobile' and 'stable' pools	785:811	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	3	9	theme	C	598:598	arg1	parameters					609:618	13 C dynamics parameters	595:618	13 C dynamics parameters	595:618	C contents, isotopic compositions and 13 C dynamics parameters were determined in bulk foliage, PF and starch.
27513732	6	10	theme	residence	1150:1158	arg1	MRT					1167:1169	MRT	1167:1169	MRT	1167:1169	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	6	10	theme	residence	1150:1158	arg1	times					1160:1164	Mean 13 C residence times	1140:1164	Mean 13 C residence times (MRT)	1140:1170	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	8	11	attach	linked	1469:1474	arg2	variations					1384:1393	Seasonal variations	1375:1393	Seasonal variations of 13 C partitioning	1375:1414	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	8	11	attach	linked	1469:1474	arg1	differences					1492:1502	phenological differences	1479:1502	phenological differences between deciduous and evergreen trees	1479:1540	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	8	11	attach	linked	1469:1474	arg2	dynamics					1420:1427	dynamics	1420:1427	dynamics	1420:1427	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	4	12	theme	bulk	695:698	arg1	foliage					700:706	bulk foliage	695:706	bulk foliage	695:706	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	4	13	from	maximal	858:864	arg1	beech					869:873	beech leaves	869:880	beech leaves in May	869:887	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	8	14	theme	phenological	1479:1490	arg1	differences					1492:1502	phenological differences	1479:1502	phenological differences between deciduous and evergreen trees	1479:1540	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	2	15	theme	chase	543:547	arg1	period					549:554	a 10-d chase period	536:554	a 10-d chase period	536:554	Polar fraction (PF, including soluble sugars, amino acids and organic acids) and starch were purified from foliage sampled during a 10-d chase period.
27513732	6	16	theme	C	1148:1148	arg1	MRT					1167:1169	MRT	1167:1169	MRT	1167:1169	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	6	16	theme	C	1148:1148	arg1	times					1160:1164	Mean 13 C residence times	1140:1164	Mean 13 C residence times (MRT)	1140:1170	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	6	17	from	growth	1259:1264	arg1	species					1274:1280	both species	1269:1280	both species	1269:1280	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	5	18	theme	needle	1093:1098	arg1	photosynthates					1100:1113	previous-year needle photosynthates	1079:1113	previous-year needle photosynthates 2 months after budburst	1079:1137	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	0	19	theme	adult	112:116	arg1	beech					118:122	adult beech	112:122	adult beech	112:122	Seasonal variations drive short-term dynamics and partitioning of recently assimilated carbon in the foliage of adult beech and pine.
27513732	5	20	from	sink	951:954	arg1	species					964:970	both species	959:970	both species	959:970	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	8	21	theme	C	1401:1401	arg1	partitioning					1403:1414	13 C partitioning	1398:1414	13 C partitioning	1398:1414	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	2	22	dep	fraction	412:419	arg1	PF					422:423	PF	422:423	PF	422:423	Polar fraction (PF, including soluble sugars, amino acids and organic acids) and starch were purified from foliage sampled during a 10-d chase period.
27513732	4	23	from	amount	685:690	arg1	foliage					700:706	bulk foliage	695:706	bulk foliage	695:706	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	7	24	theme	late	1286:1289	arg1	summer					1291:1296	late summer	1286:1296	late summer	1286:1296	In late summer, MRT differed between senescing beech leaves and overwintering pine needles.
27513732	5	25	from	Early	890:894	arg1	season					911:916	the growing season	899:916	the growing season	899:916	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	6	26	theme	photosynthate	1212:1224	arg1	export					1226:1231	fast photosynthate export	1207:1231	fast photosynthate export	1207:1231	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	0	27	theme	carbon	87:92	arg1	partitioning					50:61	partitioning	50:61	partitioning of recently assimilated carbon	50:92	Seasonal variations drive short-term dynamics and partitioning of recently assimilated carbon in the foliage of adult beech and pine.
27513732	0	27	theme	carbon	87:92	arg1	dynamics					37:44	short-term dynamics	26:44	short-term dynamics	26:44	Seasonal variations drive short-term dynamics and partitioning of recently assimilated carbon in the foliage of adult beech and pine.
27513732	2	28	theme	organic	468:474	arg1	acids					476:480	organic acids	468:480	organic acids	468:480	Polar fraction (PF, including soluble sugars, amino acids and organic acids) and starch were purified from foliage sampled during a 10-d chase period.
27513732	4	29	theme	13	680:681	arg1	C					683:683	C	683:683	C	683:683	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	0	30	theme	Seasonal	0:7	arg1	variations					9:18	Seasonal variations	0:18	Seasonal variations	0:18	Seasonal variations drive short-term dynamics and partitioning of recently assimilated carbon in the foliage of adult beech and pine.
27513732	6	31	from	summer	1188:1193	arg1	minimal					1177:1183	minimal	1177:1183	minimal	1177:1183	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	4	32	theme	mobile	786:791	arg1	pools					807:811	'mobile' and 'stable' pools	785:811	'mobile' and 'stable' pools	785:811	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	8	33	from	dynamics	1420:1427	arg1	foliage					1449:1455	field-grown tree foliage	1432:1455	field-grown tree foliage	1432:1455	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	0	34	theme	short-term	26:35	arg1	dynamics					37:44	short-term dynamics	26:44	short-term dynamics	26:44	Seasonal variations drive short-term dynamics and partitioning of recently assimilated carbon in the foliage of adult beech and pine.
27513732	1	35	theme	different	257:265	arg1	stages					280:285	different phenological stages	257:285	different phenological stages	257:285	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	4	36	dep	beech	869:873	arg1	leaves					875:880	leaves	875:880	leaves	875:880	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	8	37	theme	partitioning	1403:1414	arg1	dynamics					1420:1427	dynamics	1420:1427	dynamics	1420:1427	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	8	37	theme	partitioning	1403:1414	arg1	variations					1384:1393	Seasonal variations	1375:1393	Seasonal variations of 13 C partitioning	1375:1414	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	6	38	theme	supply	1236:1241	arg1	growth					1259:1264	supply perennial organ growth	1236:1264	supply perennial organ growth in both species	1236:1280	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	1	39	theme	adult	195:199	arg1	beeches					201:207	adult beeches	195:207	adult beeches (Fagus sylvatica)	195:225	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	1	39	theme	adult	195:199	arg1	Fagus					210:214	Fagus	210:214	Fagus	210:214	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	1	40	theme	phenological	267:278	arg1	stages					280:285	different phenological stages	257:285	different phenological stages	257:285	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	0	41	theme	beech	118:122	arg1	foliage					101:107	the foliage	97:107	the foliage of adult beech and pine	97:131	Seasonal variations drive short-term dynamics and partitioning of recently assimilated carbon in the foliage of adult beech and pine.
27513732	1	42	theme	carbon	384:389	arg1	dynamics					334:341	seasonal and interspecific short-term dynamics	296:341	seasonal and interspecific short-term dynamics	296:341	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	1	42	theme	carbon	384:389	arg1	partitioning					347:358	partitioning	347:358	partitioning of recently assimilated carbon (C)	347:393	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	4	43	theme	C	683:683	arg1	amount					685:690	13 C amount	680:690	13 C amount in bulk foliage	680:706	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	5	44	from	season	911:916	arg1	Early					890:894	Early	890:894	Early	890:894	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	8	45	theme	13	1398:1399	arg1	C					1401:1401	C	1401:1401	C	1401:1401	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	7	46	dep	beech	1330:1334	arg1	leaves					1336:1341	leaves	1336:1341	leaves	1336:1341	In late summer, MRT differed between senescing beech leaves and overwintering pine needles.
27513732	4	47	theme	latter	845:850	arg1	maximal					858:864	maximal	858:864	maximal	858:864	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	4	47	theme	latter	845:850	arg1	proportion					827:836	the relative proportion	814:836	the relative proportion of the latter	814:850	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	3	48	theme	dynamics	600:607	arg1	parameters					609:618	13 C dynamics parameters	595:618	13 C dynamics parameters	595:618	C contents, isotopic compositions and 13 C dynamics parameters were determined in bulk foliage, PF and starch.
27513732	4	49	theme	13	759:760	arg1	C					762:762	C	762:762	C	762:762	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	6	50	theme	Mean	1140:1143	arg1	MRT					1167:1169	MRT	1167:1169	MRT	1167:1169	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	6	50	theme	Mean	1140:1143	arg1	times					1160:1164	Mean 13 C residence times	1140:1164	Mean 13 C residence times (MRT)	1140:1170	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	6	51	theme	organ	1253:1257	arg1	growth					1259:1264	supply perennial organ growth	1236:1264	supply perennial organ growth in both species	1236:1280	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	0	52	theme	pine	128:131	arg1	foliage					101:107	the foliage	97:107	the foliage of adult beech and pine	97:131	Seasonal variations drive short-term dynamics and partitioning of recently assimilated carbon in the foliage of adult beech and pine.
27513732	3	53	theme	C	557:557	arg1	contents					559:566	C contents	557:566	C contents	557:566	C contents, isotopic compositions and 13 C dynamics parameters were determined in bulk foliage, PF and starch.
27513732	6	54	theme	fast	1207:1210	arg1	export					1226:1231	fast photosynthate export	1207:1231	fast photosynthate export	1207:1231	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	2	55	theme	soluble	436:442	arg1	sugars					444:449	soluble sugars	436:449	soluble sugars	436:449	Polar fraction (PF, including soluble sugars, amino acids and organic acids) and starch were purified from foliage sampled during a 10-d chase period.
27513732	8	56	theme	tree	1444:1447	arg1	foliage					1449:1455	field-grown tree foliage	1432:1455	field-grown tree foliage	1432:1455	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	2	57	theme	10-d	538:541	arg1	period					549:554	a 10-d chase period	536:554	a 10-d chase period	536:554	Polar fraction (PF, including soluble sugars, amino acids and organic acids) and starch were purified from foliage sampled during a 10-d chase period.
27513732	3	58	theme	13	595:596	arg1	C					598:598	C	598:598	C	598:598	C contents, isotopic compositions and 13 C dynamics parameters were determined in bulk foliage, PF and starch.
27513732	5	59	theme	new	919:921	arg1	sink					951:954	a strong C sink	940:954	a strong C sink in both species	940:970	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	5	59	theme	new	919:921	arg1	foliage					923:929	new foliage	919:929	new foliage	919:929	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	8	60	from	variations	1384:1393	arg1	foliage					1449:1455	field-grown tree foliage	1432:1455	field-grown tree foliage	1432:1455	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	1	61	theme	seasonal	296:303	arg1	dynamics					334:341	seasonal and interspecific short-term dynamics	296:341	seasonal and interspecific short-term dynamics	296:341	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	4	62	theme	C	762:762	arg1	partitioning					764:775	13 C partitioning	759:775	13 C partitioning between 'mobile' and 'stable' pools	759:811	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	4	63	from	Decrease	668:675	arg1	amount					685:690	13 C amount	680:690	13 C amount in bulk foliage	680:706	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	5	64	theme	growing	903:909	arg1	season					911:916	the growing season	899:916	the growing season	899:916	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	4	65	theme	relative	818:825	arg1	maximal					858:864	maximal	858:864	maximal	858:864	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	4	65	theme	relative	818:825	arg1	proportion					827:836	the relative proportion	814:836	the relative proportion of the latter	814:850	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	4	66	theme	two-pool	719:726	arg1	model					740:744	a two-pool exponential model	717:744	a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools	717:811	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	3	67	theme	isotopic	569:576	arg1	compositions					578:589	isotopic compositions	569:589	isotopic compositions	569:589	C contents, isotopic compositions and 13 C dynamics parameters were determined in bulk foliage, PF and starch.
27513732	8	68	theme	deciduous	1512:1520	arg1	trees					1536:1540	deciduous and evergreen trees	1512:1540	deciduous and evergreen trees	1512:1540	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	8	69	theme	evergreen	1526:1534	arg1	trees					1536:1540	deciduous and evergreen trees	1512:1540	deciduous and evergreen trees	1512:1540	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	5	70	dep	months	1117:1122	arg1	budburst					1130:1137	budburst	1130:1137	budburst	1130:1137	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	1	71	dep	Fagus	210:214	arg1	sylvatica					216:224	Fagus sylvatica	210:224	Fagus sylvatica	210:224	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	1	72	from	in situ	184:190	arg1	beeches					201:207	adult beeches	195:207	adult beeches (Fagus sylvatica)	195:225	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	1	72	from	in situ	184:190	arg1	pines					231:235	pines	231:235	pines (Pinus pinaster)	231:252	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	1	72	from	in situ	184:190	arg1	pinaster					244:251	pinaster	244:251	pinaster	244:251	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	1	72	from	in situ	184:190	arg1	Fagus					210:214	Fagus	210:214	Fagus	210:214	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	0	73	theme	assimilated	75:85	arg1	carbon					87:92	recently assimilated carbon	66:92	recently assimilated carbon	66:92	Seasonal variations drive short-term dynamics and partitioning of recently assimilated carbon in the foliage of adult beech and pine.
27513732	5	74	theme	strong	942:947	arg1	sink					951:954	a strong C sink	940:954	a strong C sink in both species	940:970	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	5	74	theme	strong	942:947	arg1	foliage					923:929	new foliage	919:929	new foliage	919:929	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	6	75	from	minimal	1177:1183	arg1	summer					1188:1193	summer	1188:1193	summer	1188:1193	Mean 13 C residence times (MRT) were minimal in summer, indicating fast photosynthate export to supply perennial organ growth in both species.
27513732	2	76	theme	Polar	406:410	arg1	fraction					412:419	Polar fraction	406:419	Polar fraction (PF, including soluble sugars, amino acids and organic acids)	406:481	Polar fraction (PF, including soluble sugars, amino acids and organic acids) and starch were purified from foliage sampled during a 10-d chase period.
27513732	7	77	theme	pine	1361:1364	arg1	needles					1366:1372	pine needles	1361:1372	pine needles	1361:1372	In late summer, MRT differed between senescing beech leaves and overwintering pine needles.
27513732	5	78	theme	previous-year	1079:1091	arg1	photosynthates					1100:1113	previous-year needle photosynthates	1079:1113	previous-year needle photosynthates 2 months after budburst	1079:1137	Early in the growing season, new foliage acted as a strong C sink in both species, but although young leaves and needles were already photosynthesizing, the latter were still supplied with previous-year needle photosynthates 2 months after budburst.
27513732	1	79	theme	interspecific	309:321	arg1	dynamics					334:341	seasonal and interspecific short-term dynamics	296:341	seasonal and interspecific short-term dynamics	296:341	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	4	80	theme	exponential	728:738	arg1	model					740:744	a two-pool exponential model	717:744	a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools	717:811	Decrease in 13 C amount in bulk foliage followed a two-pool exponential model highlighting 13 C partitioning between 'mobile' and 'stable' pools, the relative proportion of the latter being maximal in beech leaves in May.
27513732	1	81	theme	CO2	137:139	arg1	experiments					157:167	13 CO2 pulse-labelling experiments	134:167	13 CO2 pulse-labelling experiments	134:167	13 CO2 pulse-labelling experiments were performed in situ on adult beeches (Fagus sylvatica) and pines (Pinus pinaster) at different phenological stages to study seasonal and interspecific short-term dynamics and partitioning of recently assimilated carbon (C) in leaves.
27513732	8	82	theme	Seasonal	1375:1382	arg1	variations					1384:1393	Seasonal variations	1375:1393	Seasonal variations of 13 C partitioning	1375:1414	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27513732	8	83	theme	field-grown	1432:1442	arg1	foliage					1449:1455	field-grown tree foliage	1432:1455	field-grown tree foliage	1432:1455	Seasonal variations of 13 C partitioning and dynamics in field-grown tree foliage are closely linked to phenological differences between deciduous and evergreen trees.
27773489	7	0	theme	double	1026:1031	arg1	group					1040:1044	the double sample group	1022:1044	the double sample group	1022:1044	Histologically the best results were obtained in the double sample group: 4.5 (3.3-5.0).
27773489	5	1	located	observed	833:840	arg2	gap					825:827	RESULTS A gap	815:827	RESULTS A gap	815:827	RESULTS A gap was observed in all cases 4 weeks after supraspinatus detachment.
27773489	5	1	located	observed	833:840	arg1	cases					849:853	all cases	845:853	all cases	845:853	RESULTS A gap was observed in all cases 4 weeks after supraspinatus detachment.
27773489	2	2	from	use	153:155	arg1	carrier					173:179	a carrier	171:179	a carrier	171:179	It is hypothesised that the use of rhBMP-2 in a carrier could improve the biomechanical and histological properties of the repair.
27773489	5	3	theme	A	823:823	arg1	gap					825:827	RESULTS A gap	815:827	RESULTS A gap	815:827	RESULTS A gap was observed in all cases 4 weeks after supraspinatus detachment.
27773489	6	4	with	%	922:922	arg1	them					937:940	them	937:940	them	937:940	The re-rupture rate was 7.5%, with 20% of them in the control-alginate Group.
27773489	6	4	with	%	922:922	arg1	%					932:932	20%	930:932	20% of them in the control-alginate Group	930:970	The re-rupture rate was 7.5%, with 20% of them in the control-alginate Group.
27773489	9	5	theme	histological	1279:1290	arg1	properties					1292:1301	the biomechanical and histological properties	1257:1301	the biomechanical and histological properties of the repair site	1257:1320	CONCLUSIONS The use of alginate-chitin carrier with rhBMP-2 improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear.
27773489	3	6	contain	have	733:736	arg2	rhBMP-2					738:744	rhBMP-2	738:744	rhBMP-2	738:744	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	6	contain	have	733:736	arg1	study					301:305	Controlled experimental study	277:305	Controlled experimental study conducted on 40 rats with section	277:339	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	6	contain	have	733:736	arg1	MATERIAL					256:263	MATERIAL	256:263	MATERIAL	256:263	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	6	contain	have	733:736	arg1	repair					345:350	repair	345:350	repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin)	345:731	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	6	contain	have	733:736	arg1	METHODS					269:275	METHODS	269:275	METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin)	269:731	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	9	7	theme	site	1317:1320	arg1	properties					1292:1301	the biomechanical and histological properties	1257:1301	the biomechanical and histological properties of the repair site	1257:1320	CONCLUSIONS The use of alginate-chitin carrier with rhBMP-2 improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear.
27773489	3	8	dep	METHODS	269:275	arg1	METHODS					269:275	METHODS	269:275	METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin)	269:731	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	8	dep	METHODS	269:275	arg1	study					301:305	Controlled experimental study	277:305	Controlled experimental study conducted on 40 rats with section	277:339	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	8	dep	METHODS	269:275	arg1	repair					345:350	repair	345:350	repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin)	345:731	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	5	9	theme	RESULTS	815:821	arg1	gap					825:827	RESULTS A gap	815:827	RESULTS A gap	815:827	RESULTS A gap was observed in all cases 4 weeks after supraspinatus detachment.
27773489	1	10	theme	AIM	60:62	arg1	cuff					72:75	AIM Rotator cuff	60:75	AIM Rotator cuff	60:75	AIM Rotator cuff repairs have shown a high level of re-ruptures.
27773489	9	11	with	use	1204:1206	arg1	rhBMP-2					1240:1246	rhBMP-2	1240:1246	rhBMP-2	1240:1246	CONCLUSIONS The use of alginate-chitin carrier with rhBMP-2 improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear.
27773489	9	12	theme	biomechanical	1261:1273	arg1	properties					1292:1301	the biomechanical and histological properties	1257:1301	the biomechanical and histological properties of the repair site	1257:1320	CONCLUSIONS The use of alginate-chitin carrier with rhBMP-2 improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear.
27773489	3	13	theme	rhBMP-2	625:631	arg1	application					606:616	the alginate; Group 4 (chitin-control) application	567:616	the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin	567:645	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	1	14	theme	Rotator	64:70	arg1	cuff					72:75	AIM Rotator cuff	60:75	AIM Rotator cuff	60:75	AIM Rotator cuff repairs have shown a high level of re-ruptures.
27773489	8	15	dep	69.4	1142:1145	arg1	to					1139:1140	to	1139:1140	to	1139:1140	Double sample were also able to support higher loads to failure: 62.9N (59.8 to 69.4) with lower rigidity 12.7 (9.7 to 15.9).
27773489	5	16	dep	weeks	857:861	arg1	detachment					883:892	supraspinatus detachment	869:892	supraspinatus detachment	869:892	RESULTS A gap was observed in all cases 4 weeks after supraspinatus detachment.
27773489	8	17	theme	rigidity	1159:1166	arg1	12.7					1168:1171	lower rigidity 12.7	1153:1171	lower rigidity 12.7	1153:1171	Double sample were also able to support higher loads to failure: 62.9N (59.8 to 69.4) with lower rigidity 12.7 (9.7 to 15.9).
27773489	3	18	theme	groups	413:418	arg1	one					401:403	one	401:403	one	401:403	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	18	theme	groups	413:418	arg1	groups					413:418	five groups	408:418	five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier	408:709	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	7	19	theme	best	992:995	arg1	results					997:1003	the best results	988:1003	the best results	988:1003	Histologically the best results were obtained in the double sample group: 4.5 (3.3-5.0).
27773489	7	20	theme	sample	1033:1038	arg1	group					1040:1044	the double sample group	1022:1044	the double sample group	1022:1044	Histologically the best results were obtained in the double sample group: 4.5 (3.3-5.0).
27773489	3	21	theme	alginate-chitin	489:503	arg1	carrier					505:511	alginate-chitin carrier	489:511	alginate-chitin carrier	489:511	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	22	theme	double	461:466	arg1	Group					452:456	Group 2	452:458	Group 2 (double control)	452:475	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	22	theme	double	461:466	arg1	control					468:474	double control	461:474	double control	461:474	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	23	dep	one	401:403	arg1	chitin					725:730	chitin	725:730	chitin	725:730	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	23	dep	one	401:403	arg1	alginate					712:719	alginate	712:719	alginate	712:719	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	24	theme	supraspinatus	359:371	arg1	tendon					373:378	the supraspinatus tendon	355:378	the supraspinatus tendon	355:378	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	2	25	theme	rhBMP-2	160:166	arg1	use					153:155	the use	149:155	the use of rhBMP-2 in a carrier	149:179	It is hypothesised that the use of rhBMP-2 in a carrier could improve the biomechanical and histological properties of the repair.
27773489	3	26	dep	Group	452:456	arg1	Group					514:518	Group 3	514:520	Group 3 (alginate-control)	514:539	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	26	dep	Group	452:456	arg1	alginate-control					523:538	alginate-control	523:538	alginate-control	523:538	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	6	27	theme	re-rupture	899:908	arg1	rate					910:913	The re-rupture rate	895:913	The re-rupture rate	895:913	The re-rupture rate was 7.5%, with 20% of them in the control-alginate Group.
27773489	6	27	theme	re-rupture	899:908	arg1	%					922:922	7.5%	919:922	7.5%	919:922	The re-rupture rate was 7.5%, with 20% of them in the control-alginate Group.
27773489	0	28	theme	cuff	18:21	arg1	injuries					23:30	rotator cuff injuries	10:30	rotator cuff injuries	10:30	Repair of rotator cuff injuries using different composites.
27773489	8	29	dep	able	1086:1089	arg1	69.4					1142:1145	69.4	1142:1145	69.4	1142:1145	Double sample were also able to support higher loads to failure: 62.9N (59.8 to 69.4) with lower rigidity 12.7 (9.7 to 15.9).
27773489	8	29	dep	able	1086:1089	arg1	15.9					1181:1184	15.9	1181:1184	15.9	1181:1184	Double sample were also able to support higher loads to failure: 62.9N (59.8 to 69.4) with lower rigidity 12.7 (9.7 to 15.9).
27773489	8	29	dep	able	1086:1089	arg1	62.9N					1127:1131	62.9N	1127:1131	62.9N	1127:1131	Double sample were also able to support higher loads to failure: 62.9N (59.8 to 69.4) with lower rigidity 12.7 (9.7 to 15.9).
27773489	4	30	theme	histological	767:778	arg1	analysis					780:787	A biomechanical and histological analysis	747:787	A biomechanical and histological analysis	747:787	A biomechanical and histological analysis was performed at 4 weeks.
27773489	9	31	dep	CONCLUSIONS	1188:1198	arg1	improves					1248:1255	improves	1248:1255	improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear	1248:1351	CONCLUSIONS The use of alginate-chitin carrier with rhBMP-2 improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear.
27773489	6	32	theme	control-alginate	949:964	arg1	Group					966:970	the control-alginate Group	945:970	the control-alginate Group	945:970	The re-rupture rate was 7.5%, with 20% of them in the control-alginate Group.
27773489	3	33	with	rats	323:326	arg1	section					333:339	section	333:339	section	333:339	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	8	34	theme	Double	1062:1067	arg1	sample					1069:1074	Double sample	1062:1074	Double sample	1062:1074	Double sample were also able to support higher loads to failure: 62.9N (59.8 to 69.4) with lower rigidity 12.7 (9.7 to 15.9).
27773489	0	35	theme	rotator	10:16	arg1	injuries					23:30	rotator cuff injuries	10:30	rotator cuff injuries	10:30	Repair of rotator cuff injuries using different composites.
27773489	2	36	theme	repair	248:253	arg1	properties					230:239	the biomechanical and histological properties	195:239	the biomechanical and histological properties of the repair	195:253	It is hypothesised that the use of rhBMP-2 in a carrier could improve the biomechanical and histological properties of the repair.
27773489	9	37	theme	cuff	1343:1346	arg1	tear					1348:1351	a chronic rotator cuff tear	1325:1351	a chronic rotator cuff tear	1325:1351	CONCLUSIONS The use of alginate-chitin carrier with rhBMP-2 improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear.
27773489	9	38	theme	alginate-chitin	1211:1225	arg1	carrier					1227:1233	alginate-chitin carrier	1211:1233	alginate-chitin carrier	1211:1233	CONCLUSIONS The use of alginate-chitin carrier with rhBMP-2 improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear.
27773489	3	39	theme	randomisation	384:396	arg1	METHODS					269:275	METHODS	269:275	METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin)	269:731	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	39	theme	randomisation	384:396	arg1	study					301:305	Controlled experimental study	277:305	Controlled experimental study conducted on 40 rats with section	277:339	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	39	theme	randomisation	384:396	arg1	repair					345:350	repair	345:350	repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin)	345:731	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	8	40	dep	15.9	1181:1184	arg1	to					1178:1179	to	1178:1179	to	1178:1179	Double sample were also able to support higher loads to failure: 62.9N (59.8 to 69.4) with lower rigidity 12.7 (9.7 to 15.9).
27773489	2	41	theme	biomechanical	199:211	arg1	properties					230:239	the biomechanical and histological properties	195:239	the biomechanical and histological properties of the repair	195:253	It is hypothesised that the use of rhBMP-2 in a carrier could improve the biomechanical and histological properties of the repair.
27773489	9	42	theme	carrier	1227:1233	arg1	use					1204:1206	The use	1200:1206	The use of alginate-chitin carrier with rhBMP-2	1200:1246	CONCLUSIONS The use of alginate-chitin carrier with rhBMP-2 improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear.
27773489	0	43	theme	injuries	23:30	arg1	Repair					0:5	Repair	0:5	Repair of rotator cuff injuries	0:30	Repair of rotator cuff injuries using different composites.
27773489	8	44	theme	lower	1153:1157	arg1	12.7					1168:1171	lower rigidity 12.7	1153:1171	lower rigidity 12.7	1153:1171	Double sample were also able to support higher loads to failure: 62.9N (59.8 to 69.4) with lower rigidity 12.7 (9.7 to 15.9).
27773489	3	45	theme	tendon	373:378	arg1	METHODS					269:275	METHODS	269:275	METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin)	269:731	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	45	theme	tendon	373:378	arg1	study					301:305	Controlled experimental study	277:305	Controlled experimental study conducted on 40 rats with section	277:339	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	45	theme	tendon	373:378	arg1	repair					345:350	repair	345:350	repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin)	345:731	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	46	theme	Controlled	277:286	arg1	METHODS					269:275	METHODS	269:275	METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin)	269:731	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	46	theme	Controlled	277:286	arg1	study					301:305	Controlled experimental study	277:305	Controlled experimental study conducted on 40 rats with section	277:339	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	6	47	from	%	932:932	arg1	Group					966:970	the control-alginate Group	945:970	the control-alginate Group	945:970	The re-rupture rate was 7.5%, with 20% of them in the control-alginate Group.
27773489	1	48	theme	high	98:101	arg1	level					103:107	a high level	96:107	a high level of re-ruptures	96:122	AIM Rotator cuff repairs have shown a high level of re-ruptures.
27773489	0	49	theme	different	38:46	arg1	composites					48:57	different composites	38:57	different composites	38:57	Repair of rotator cuff injuries using different composites.
27773489	3	50	theme	experimental	288:299	arg1	METHODS					269:275	METHODS	269:275	METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin)	269:731	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	50	theme	experimental	288:299	arg1	study					301:305	Controlled experimental study	277:305	Controlled experimental study conducted on 40 rats with section	277:339	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	5	51	theme	supraspinatus	869:881	arg1	detachment					883:892	supraspinatus detachment	869:892	supraspinatus detachment	869:892	RESULTS A gap was observed in all cases 4 weeks after supraspinatus detachment.
27773489	3	52	dep	groups	413:418	arg1	Group					421:425	Group 1	421:427	five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier	408:709	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	52	dep	groups	413:418	arg1	control					430:436	control	430:436	control	430:436	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	52	dep	groups	413:418	arg1	sample					668:673	double sample	661:673	double sample	661:673	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	6	53	theme	them	937:940	arg1	them					937:940	them	937:940	them	937:940	The re-rupture rate was 7.5%, with 20% of them in the control-alginate Group.
27773489	6	53	theme	them	937:940	arg1	%					932:932	20%	930:932	20% of them in the control-alginate Group	930:970	The re-rupture rate was 7.5%, with 20% of them in the control-alginate Group.
27773489	9	54	theme	repair	1310:1315	arg1	site					1317:1320	the repair site	1306:1320	the repair site	1306:1320	CONCLUSIONS The use of alginate-chitin carrier with rhBMP-2 improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear.
27773489	4	55	theme	biomechanical	749:761	arg1	analysis					780:787	A biomechanical and histological analysis	747:787	A biomechanical and histological analysis	747:787	A biomechanical and histological analysis was performed at 4 weeks.
27773489	3	56	theme	Group	581:585	arg1	application					606:616	the alginate; Group 4 (chitin-control) application	567:616	the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin	567:645	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	9	57	theme	chronic	1327:1333	arg1	tear					1348:1351	a chronic rotator cuff tear	1325:1351	a chronic rotator cuff tear	1325:1351	CONCLUSIONS The use of alginate-chitin carrier with rhBMP-2 improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear.
27773489	9	58	theme	rotator	1335:1341	arg1	tear					1348:1351	a chronic rotator cuff tear	1325:1351	a chronic rotator cuff tear	1325:1351	CONCLUSIONS The use of alginate-chitin carrier with rhBMP-2 improves the biomechanical and histological properties of the repair site in a chronic rotator cuff tear.
27773489	7	59	dep	obtained	1010:1017	arg1	3.3-5.0					1052:1058	3.3-5.0	1052:1058	3.3-5.0	1052:1058	Histologically the best results were obtained in the double sample group: 4.5 (3.3-5.0).
27773489	7	59	dep	obtained	1010:1017	arg1	4.5					1047:1049	4.5	1047:1049	4.5	1047:1049	Histologically the best results were obtained in the double sample group: 4.5 (3.3-5.0).
27773489	3	60	dep	Group	421:425	arg1	added					558:562	added	558:562	was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin	554:645	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	60	dep	Group	421:425	arg1	Group					652:656	Group 5	652:658	Group 5	652:658	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	60	dep	Group	421:425	arg1	suture					444:449	suture	444:449	suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin	444:645	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	60	dep	Group	421:425	arg1	components					685:694	The two components	677:694	five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier	408:709	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	1	61	theme	re-ruptures	112:122	arg1	level					103:107	a high level	96:107	a high level of re-ruptures	96:122	AIM Rotator cuff repairs have shown a high level of re-ruptures.
27773489	3	62	theme	alginate	571:578	arg1	application					606:616	the alginate; Group 4 (chitin-control) application	567:616	the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin	567:645	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	2	63	theme	histological	217:228	arg1	properties					230:239	the biomechanical and histological properties	195:239	the biomechanical and histological properties of the repair	195:253	It is hypothesised that the use of rhBMP-2 in a carrier could improve the biomechanical and histological properties of the repair.
27773489	3	64	theme	carrier	703:709	arg1	components					685:694	The two components	677:694	five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier	408:709	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	65	theme	double	661:666	arg1	Group					421:425	Group 1	421:427	five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier	408:709	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	65	theme	double	661:666	arg1	sample					668:673	double sample	661:673	double sample	661:673	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	3	66	theme	chitin-control	590:603	arg1	application					606:616	the alginate; Group 4 (chitin-control) application	567:616	the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin	567:645	MATERIAL AND METHODS Controlled experimental study conducted on 40 rats with section and repair of the supraspinatus tendon and randomisation to one of five groups: Group 1 (control) only suture; Group 2 (double control), suture and alginate-chitin carrier; Group 3 (alginate-control), the rhBMP-2 was added to the alginate; Group 4 (chitin-control) application of the rhBMP-2 to the chitin, and Group 5 (double sample): The two components of the carrier (alginate and chitin) have rhBMP-2.
27773489	8	67	theme	higher	1102:1107	arg1	loads					1109:1113	higher loads	1102:1113	higher loads	1102:1113	Double sample were also able to support higher loads to failure: 62.9N (59.8 to 69.4) with lower rigidity 12.7 (9.7 to 15.9).
25433275	0	0	theme	calcium	72:78	arg1	carbonate					80:88	amorphous calcium carbonate	62:88	amorphous calcium carbonate	62:88	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.
25433275	1	1	from	deposition	297:306	arg1	chitin-matrix					360:372	a chitin-matrix	358:372	a chitin-matrix during time	358:384	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	2	2	from	consequences	406:417	arg1	composition					469:479	composition	469:479	composition	469:479	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	2	2	from	consequences	406:417	arg1	structure					455:463	local structure	449:463	local structure	449:463	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	1	3	dep	deposition	297:306	arg1	the					282:284	the	282:284	the	282:284	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	1	4	theme	calcium	236:242	arg1	carbonate					244:252	calcium carbonate	236:252	mostly calcium carbonate	229:252	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	0	5	theme	amorphous	62:70	arg1	carbonate					80:88	amorphous calcium carbonate	62:88	amorphous calcium carbonate	62:88	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.
25433275	1	6	theme	amorphous	329:337	arg1	spheres					347:353	amorphous mineral spheres	329:353	amorphous mineral spheres on a chitin-matrix during time	329:384	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	2	7	theme	growth	435:440	arg1	consequences					406:417	the consequences	402:417	the consequences of this layered growth on the local structure and composition of the gastrolith	402:497	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	2	8	dep	structure	455:463	arg1	the					445:447	the	445:447	the	445:447	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	2	9	theme	spatially-resolved	513:530	arg1	analysis					557:564	spatially-resolved Raman, X-ray and SEM-EDS analysis	513:564	analysis	557:564	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	1	10	theme	mineral	339:345	arg1	spheres					347:353	amorphous mineral spheres	329:353	amorphous mineral spheres on a chitin-matrix during time	329:384	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	2	11	theme	gastrolith	587:596	arg1	cross-sections					598:611	complete pre-molt gastrolith cross-sections	569:611	complete pre-molt gastrolith cross-sections	569:611	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	0	12	theme	carbonate	80:88	arg1	stability					49:57	about the stability	39:57	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.	0:111	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.
25433275	1	13	theme	spheres	347:353	arg1	densification					312:324	densification	312:324	densification	312:324	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	1	13	theme	spheres	347:353	arg1	deposition					297:306	continuous deposition	286:306	continuous deposition	286:306	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	2	14	theme	pre-molt	578:585	arg1	cross-sections					598:611	complete pre-molt gastrolith cross-sections	569:611	complete pre-molt gastrolith cross-sections	569:611	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	2	15	theme	X-ray	539:543	arg1	analysis					557:564	spatially-resolved Raman, X-ray and SEM-EDS analysis	513:564	analysis	557:564	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	0	16	theme	role	94:97	arg1	stability					49:57	about the stability	39:57	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.	0:111	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.
25433275	3	17	theme	proteins	719:726	arg1	abundance					647:655	especially the abundance	632:655	especially the abundance of inorganic phosphate, phosphoenolpyruvate (PEP)/citrate and proteins	632:726	Results show that especially the abundance of inorganic phosphate, phosphoenolpyruvate (PEP)/citrate and proteins is not uniform throughout the organ but changes from layer to layer.
25433275	1	18	from	chitin-matrix	360:372	arg1	densification					312:324	densification	312:324	densification	312:324	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	1	18	from	chitin-matrix	360:372	arg1	deposition					297:306	continuous deposition	286:306	continuous deposition	286:306	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	4	19	from	stabilization	845:857	arg1	gastrolith					866:875	the gastrolith	862:875	the gastrolith	862:875	Based on these results we can conclude that ACC stabilization in the gastrolith takes place by more than one compound and not by only one of these additives.
25433275	1	20	from	studies	122:128	arg1	gastroliths					142:152	pre-molt gastroliths	133:152	pre-molt gastroliths	133:152	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	1	21	theme	typical	167:173	arg1	morphology					186:195	a typical onion-like morphology	165:195	a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin	165:264	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	0	22	theme	Layered	0:6	arg1	growth					8:13	Layered growth	0:13	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.	0:111	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.
25433275	1	23	theme	chitin	259:264	arg1	layers					200:205	layers	200:205	layers of amorphous mineral (mostly calcium carbonate) and chitin	200:264	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	4	24	theme	ACC	841:843	arg1	stabilization					845:857	ACC stabilization	841:857	ACC stabilization in the gastrolith	841:875	Based on these results we can conclude that ACC stabilization in the gastrolith takes place by more than one compound and not by only one of these additives.
25433275	1	25	theme	onion-like	175:184	arg1	morphology					186:195	a typical onion-like morphology	165:195	a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin	165:264	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	0	26	theme	crayfish	18:25	arg1	gastrolith					27:36	crayfish gastrolith	18:36	crayfish gastrolith	18:36	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.
25433275	0	27	theme	additives	102:110	arg1	role					94:97	role	94:97	role of additives	94:110	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.
25433275	0	27	theme	additives	102:110	arg1	carbonate					80:88	amorphous calcium carbonate	62:88	amorphous calcium carbonate	62:88	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.
25433275	4	28	theme	additives	944:952	arg1	one					931:933	one	931:933	one	931:933	Based on these results we can conclude that ACC stabilization in the gastrolith takes place by more than one compound and not by only one of these additives.
25433275	4	28	theme	additives	944:952	arg1	additives					944:952	these additives	938:952	these additives	938:952	Based on these results we can conclude that ACC stabilization in the gastrolith takes place by more than one compound and not by only one of these additives.
25433275	3	29	theme	inorganic	660:668	arg1	phosphate					670:678	inorganic phosphate	660:678	inorganic phosphate	660:678	Results show that especially the abundance of inorganic phosphate, phosphoenolpyruvate (PEP)/citrate and proteins is not uniform throughout the organ but changes from layer to layer.
25433275	1	30	theme	layers	200:205	arg1	morphology					186:195	a typical onion-like morphology	165:195	a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin	165:264	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	0	31	theme	gastrolith	27:36	arg1	growth					8:13	Layered growth	0:13	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.	0:111	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.
25433275	2	32	theme	Raman	532:536	arg1	analysis					557:564	spatially-resolved Raman, X-ray and SEM-EDS analysis	513:564	analysis	557:564	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	1	33	from	spheres	347:353	arg1	chitin-matrix					360:372	a chitin-matrix	358:372	a chitin-matrix during time	358:384	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	1	34	from	densification	312:324	arg1	chitin-matrix					360:372	a chitin-matrix	358:372	a chitin-matrix during time	358:384	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	1	35	theme	continuous	286:295	arg1	deposition					297:306	continuous deposition	286:306	continuous deposition	286:306	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	2	36	theme	complete	569:576	arg1	cross-sections					598:611	complete pre-molt gastrolith cross-sections	569:611	complete pre-molt gastrolith cross-sections	569:611	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	1	37	dep	mineral	220:226	arg1	carbonate					244:252	calcium carbonate	236:252	mostly calcium carbonate	229:252	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	2	38	theme	layered	427:433	arg1	growth					435:440	this layered growth	422:440	this layered growth	422:440	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	3	39	theme	phosphoenolpyruvate	681:699	arg1	/citrate					706:713	phosphoenolpyruvate (PEP)/citrate	681:713	phosphoenolpyruvate (PEP)/citrate	681:713	Results show that especially the abundance of inorganic phosphate, phosphoenolpyruvate (PEP)/citrate and proteins is not uniform throughout the organ but changes from layer to layer.
25433275	1	40	theme	Previous	113:120	arg1	studies					122:128	Previous studies	113:128	Previous studies on pre-molt gastroliths	113:152	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	2	41	theme	SEM-EDS	549:555	arg1	analysis					557:564	spatially-resolved Raman, X-ray and SEM-EDS analysis	513:564	analysis	557:564	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	3	42	theme	phosphate	670:678	arg1	abundance					647:655	especially the abundance	632:655	especially the abundance of inorganic phosphate, phosphoenolpyruvate (PEP)/citrate and proteins	632:726	Results show that especially the abundance of inorganic phosphate, phosphoenolpyruvate (PEP)/citrate and proteins is not uniform throughout the organ but changes from layer to layer.
25433275	1	43	theme	mineral	220:226	arg1	layers					200:205	layers	200:205	layers of amorphous mineral (mostly calcium carbonate) and chitin	200:264	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
25433275	2	44	theme	gastrolith	488:497	arg1	composition					469:479	composition	469:479	composition	469:479	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	2	44	theme	gastrolith	488:497	arg1	structure					455:463	local structure	449:463	local structure	449:463	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	0	45	dep	growth	8:13	arg1	stability					49:57	about the stability	39:57	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.	0:111	Layered growth of crayfish gastrolith: about the stability of amorphous calcium carbonate and role of additives.
25433275	2	46	theme	local	449:453	arg1	structure					455:463	local structure	449:463	local structure	449:463	To investigate the consequences of this layered growth on the local structure and composition of the gastrolith, we performed spatially-resolved Raman, X-ray and SEM-EDS analysis on complete pre-molt gastrolith cross-sections.
25433275	3	47	theme	/citrate	706:713	arg1	abundance					647:655	especially the abundance	632:655	especially the abundance of inorganic phosphate, phosphoenolpyruvate (PEP)/citrate and proteins	632:726	Results show that especially the abundance of inorganic phosphate, phosphoenolpyruvate (PEP)/citrate and proteins is not uniform throughout the organ but changes from layer to layer.
25433275	1	48	theme	pre-molt	133:140	arg1	gastroliths					142:152	pre-molt gastroliths	133:152	pre-molt gastroliths	133:152	Previous studies on pre-molt gastroliths have shown a typical onion-like morphology of layers of amorphous mineral (mostly calcium carbonate) and chitin, resulting from the continuous deposition and densification of amorphous mineral spheres on a chitin-matrix during time.
24650129	6	0	theme	other	1195:1199	arg1	configurations					1209:1222	other flowing configurations	1195:1222	other flowing configurations	1195:1222	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	5	1	theme	≥40	1027:1029	arg1	μg/mL					1031:1035	≥40 μg/mL	1027:1035	≥40 μg/mL	1027:1035	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	1	2	theme	polyion	157:163	arg1	detectors					213:221	detectors	213:221	detectors in a flow-injection analysis (FIA) system	213:263	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	1	2	theme	polyion	157:163	arg1	pulstrodes					199:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	3	3	theme	original	670:677	arg1	composition					688:698	its original chemical composition	666:698	its original chemical composition	666:698	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	6	4	theme	liquid	1236:1241	arg1	chromatography					1243:1256	liquid chromatography	1236:1256	liquid chromatography	1236:1256	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	4	5	theme	samples	816:822	arg1	detection					794:802	reproducible real-time detection	771:802	reproducible real-time detection of injected samples of protamine and heparin	771:847	It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h.
24650129	1	6	theme	selective	165:173	arg1	detectors					213:221	detectors	213:221	detectors in a flow-injection analysis (FIA) system	213:263	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	1	6	theme	selective	165:173	arg1	pulstrodes					199:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	5	7	theme	membrane	907:914	arg1	detector					916:923	the same membrane detector	898:923	the same membrane detector	898:923	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	6	8	theme	polyionic	1160:1168	arg1	species					1170:1176	polyionic species	1160:1176	polyionic species	1160:1176	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	0	9	from	detector	58:65	arg1	analysis					85:92	flow-injection analysis	70:92	flow-injection analysis	70:92	Polyion selective polymeric membrane-based pulstrode as a detector in flow-injection analysis.
24650129	4	10	theme	protamine	827:835	arg1	detection					794:802	reproducible real-time detection	771:802	reproducible real-time detection of injected samples of protamine and heparin	771:847	It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h.
24650129	6	11	theme	low	1181:1183	arg1	levels					1185:1190	low levels	1181:1190	low levels in other flowing configurations	1181:1222	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	2	12	theme	detection	282:290	arg1	electrode					292:300	The detection electrode	278:300	The detection electrode	278:300	The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt.
24650129	6	13	dep	such	1225:1228	arg1	as					1230:1231	as	1230:1231	as	1230:1231	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	6	14	theme	capillary	1262:1270	arg1	electrophoresis					1272:1286	capillary electrophoresis	1262:1286	capillary electrophoresis	1262:1286	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	3	15	theme	chemical	679:686	arg1	composition					688:698	its original chemical composition	666:698	its original chemical composition	666:698	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	4	16	theme	pulse	724:728	arg1	times					739:743	total pulse sequence times	718:743	total pulse sequence times	718:743	It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h.
24650129	1	17	theme	polymeric	175:183	arg1	detectors					213:221	detectors	213:221	detectors in a flow-injection analysis (FIA) system	213:263	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	1	17	theme	polymeric	175:183	arg1	pulstrodes					199:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	4	18	dep	20	858:859	arg1	to					855:856	to	855:856	to	855:856	It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h.
24650129	4	19	theme	real-time	784:792	arg1	detection					794:802	reproducible real-time detection	771:802	reproducible real-time detection of injected samples of protamine and heparin	771:847	It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h.
24650129	3	20	dep	longer	605:610	arg1	s					616:616	15 s	613:616	15 s	613:616	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	1	21	theme	membrane	185:192	arg1	detectors					213:221	detectors	213:221	detectors in a flow-injection analysis (FIA) system	213:263	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	1	21	theme	membrane	185:192	arg1	pulstrodes					199:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	5	22	theme	FIA	945:947	arg1	detection					949:957	FIA detection	945:957	FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL	945:1035	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	3	23	theme	open-circuit	526:537	arg1	pulse					539:543	an open-circuit pulse	523:543	an open-circuit pulse (0.5 s) during which the EMF of the cell is measured	523:596	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	3	23	theme	open-circuit	526:537	arg1	s					550:550	0.5 s	546:550	0.5 s	546:550	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	4	24	theme	injected	807:814	arg1	samples					816:822	injected samples	807:822	injected samples	807:822	It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h.
24650129	2	25	theme	plasticized	316:326	arg1	membrane					338:345	a plasticized polymeric membrane	314:345	a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt	314:452	The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt.
24650129	1	26	theme	type	194:197	arg1	detectors					213:221	detectors	213:221	detectors in a flow-injection analysis (FIA) system	213:263	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	1	26	theme	type	194:197	arg1	pulstrodes					199:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	5	27	theme	pulse	1084:1088	arg1	direction					1053:1061	the direction	1049:1061	the direction of the galvanostatic pulse	1049:1088	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	6	28	from	levels	1185:1190	arg1	configurations					1209:1222	other flowing configurations	1195:1222	other flowing configurations	1195:1222	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	6	28	from	levels	1185:1190	arg1	detection					1147:1155	the detection	1143:1155	the detection of polyionic species at low levels in other flowing configurations	1143:1222	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	3	29	theme	pulse	459:463	arg1	sequence					465:472	The pulse sequence	455:472	The pulse sequence used	455:477	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	0	30	theme	membrane-based	28:41	arg1	pulstrode					43:51	polymeric membrane-based pulstrode	18:51	polymeric membrane-based pulstrode	18:51	Polyion selective polymeric membrane-based pulstrode as a detector in flow-injection analysis.
24650129	6	31	from	electrophoresis	1272:1286	arg1	applicable					1129:1138	applicable	1129:1138	applicable	1129:1138	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	3	32	theme	short	490:494	arg1	s					499:499	1 s	497:499	1 s	497:499	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	3	32	theme	short	490:494	arg1	pulse					516:520	a short (1 s) galvanostatic pulse	488:520	a short (1 s) galvanostatic pulse	488:520	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	0	33	theme	polymeric	18:26	arg1	pulstrode					43:51	polymeric membrane-based pulstrode	18:51	polymeric membrane-based pulstrode	18:51	Polyion selective polymeric membrane-based pulstrode as a detector in flow-injection analysis.
24650129	6	34	from	chromatography	1243:1256	arg1	applicable					1129:1138	applicable	1129:1138	applicable	1129:1138	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	5	35	theme	same	902:905	arg1	detector					916:923	the same membrane detector	898:923	the same membrane detector	898:923	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	5	36	theme	≥10	989:991	arg1	μg/mL					993:997	≥10 μg/mL	989:997	≥10 μg/mL	989:997	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	2	37	theme	salt	449:452	arg1	salt					449:452	tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt	369:452	tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt	369:452	The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt.
24650129	2	37	theme	salt	449:452	arg1	%					364:364	10 wt %	358:364	10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt	358:452	The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt.
24650129	2	38	theme	ion-exchanger	435:447	arg1	salt					449:452	tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt	369:452	tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt	369:452	The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt.
24650129	5	39	dep	levels	982:987	arg1	μg/mL					993:997	≥10 μg/mL	989:997	≥10 μg/mL	989:997	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	5	39	dep	levels	982:987	arg1	polyanions					1003:1012	polyanions	1003:1012	polyanions at levels of ≥40 μg/mL	1003:1035	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	3	40	theme	cell	581:584	arg1	EMF					570:572	the EMF	566:572	the EMF of the cell	566:584	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	6	41	theme	flowing	1201:1207	arg1	configurations					1209:1222	other flowing configurations	1195:1222	other flowing configurations	1195:1222	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	3	42	theme	longer	605:610	arg1	pulse					634:638	a longer (15 s) potentiostatic pulse	603:638	a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition	603:698	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	4	43	theme	reproducible	771:782	arg1	detection					794:802	reproducible real-time detection	771:802	reproducible real-time detection of injected samples of protamine and heparin	771:847	It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h.
24650129	4	44	theme	heparin	841:847	arg1	detection					794:802	reproducible real-time detection	771:802	reproducible real-time detection of injected samples of protamine and heparin	771:847	It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h.
24650129	3	45	theme	potentiostatic	619:632	arg1	pulse					634:638	a longer (15 s) potentiostatic pulse	603:638	a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition	603:698	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	6	46	theme	species	1170:1176	arg1	detection					1147:1155	the detection	1143:1155	the detection of polyionic species at low levels in other flowing configurations	1143:1222	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	3	47	theme	galvanostatic	502:514	arg1	s					499:499	1 s	497:499	1 s	497:499	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	3	47	theme	galvanostatic	502:514	arg1	pulse					516:520	a short (1 s) galvanostatic pulse	488:520	a short (1 s) galvanostatic pulse	488:520	The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition.
24650129	5	48	theme	galvanostatic	1070:1082	arg1	pulse					1084:1088	the galvanostatic pulse	1066:1088	the galvanostatic pulse	1066:1088	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	5	49	theme	polycations	967:977	arg1	detection					949:957	FIA detection	945:957	FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL	945:1035	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	1	50	from	detectors	213:221	arg1	system					258:263	a flow-injection analysis (FIA) system	226:263	a flow-injection analysis (FIA) system	226:263	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	1	51	theme	polyions	125:132	arg1	detection					112:120	the detection	108:120	the detection of polyions	108:132	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	6	52	from	applicable	1129:1138	arg1	electrophoresis					1272:1286	capillary electrophoresis	1262:1286	capillary electrophoresis	1262:1286	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	6	52	from	applicable	1129:1138	arg1	chromatography					1243:1256	liquid chromatography	1236:1256	liquid chromatography	1236:1256	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	6	52	from	applicable	1129:1138	arg1	detection					1147:1155	the detection	1143:1155	the detection of polyionic species at low levels in other flowing configurations	1143:1222	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	1	53	theme	flow-injection	228:241	arg1	system					258:263	a flow-injection analysis (FIA) system	226:263	a flow-injection analysis (FIA) system	226:263	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	4	54	theme	total	718:722	arg1	times					739:743	total pulse sequence times	718:743	total pulse sequence times	718:743	It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h.
24650129	4	55	theme	sequence	730:737	arg1	times					739:743	total pulse sequence times	718:743	total pulse sequence times	718:743	It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h.
24650129	2	56	theme	polymeric	328:336	arg1	membrane					338:345	a plasticized polymeric membrane	314:345	a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt	314:452	The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt.
24650129	6	57	from	detection	1147:1155	arg1	applicable					1129:1138	applicable	1129:1138	applicable	1129:1138	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	6	57	from	detection	1147:1155	arg1	methodology					1095:1105	The methodology	1091:1105	The methodology described	1091:1115	The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
24650129	1	58	theme	analysis	243:250	arg1	system					258:263	a flow-injection analysis (FIA) system	226:263	a flow-injection analysis (FIA) system	226:263	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	0	59	theme	flow-injection	70:83	arg1	analysis					85:92	flow-injection analysis	70:92	flow-injection analysis	70:92	Polyion selective polymeric membrane-based pulstrode as a detector in flow-injection analysis.
24650129	2	60	theme	wt	361:362	arg1	salt					449:452	tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt	369:452	tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt	369:452	The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt.
24650129	2	60	theme	wt	361:362	arg1	%					364:364	10 wt %	358:364	10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt	358:452	The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt.
24650129	5	61	from	levels	982:987	arg1	detection					949:957	FIA detection	945:957	FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL	945:1035	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	5	62	theme	μg/mL	1031:1035	arg1	levels					1017:1022	levels	1017:1022	levels of ≥40 μg/mL	1017:1035	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	5	63	from	levels	1017:1022	arg1	μg/mL					993:997	≥10 μg/mL	989:997	≥10 μg/mL	989:997	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	5	63	from	levels	1017:1022	arg1	polyanions					1003:1012	polyanions	1003:1012	polyanions at levels of ≥40 μg/mL	1003:1035	Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse.
24650129	1	64	theme	reversible	146:155	arg1	detectors					213:221	detectors	213:221	detectors in a flow-injection analysis (FIA) system	213:263	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	1	64	theme	reversible	146:155	arg1	pulstrodes					199:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	fully reversible polyion selective polymeric membrane type pulstrodes	140:208	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
24650129	1	65	theme	FIA	253:255	arg1	system					258:263	a flow-injection analysis (FIA) system	226:263	a flow-injection analysis (FIA) system	226:263	A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined.
27647386	2	0	theme	-induced	481:488	arg1	polymerization					496:509	ultraviolet (UV)-induced graft polymerization	465:509	ultraviolet (UV)-induced graft polymerization	465:509	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	9	1	dep	CONCLUSIONS	1363:1373	arg1	provided					1481:1488	provided	1481:1488	provided new thoughts and experimental bases for exploring novel material applied in tissue engineering	1481:1583	CONCLUSIONS This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
27647386	9	1	dep	CONCLUSIONS	1363:1373	arg1	developed					1386:1394	developed	1386:1394	developed a simple and economical method to fabricate thermoresponsive cell culture dishes	1386:1475	CONCLUSIONS This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
27647386	4	2	theme	modified	834:841	arg1	TCPS					843:846	the modified TCPS	830:846	the modified TCPS	830:846	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	4	3	from	behaviors	769:777	arg1	surface					819:825	the surface	815:825	the surface of the modified TCPS	815:846	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	9	4	theme	tissue	1566:1571	arg1	engineering					1573:1583	tissue engineering	1566:1583	tissue engineering	1566:1583	CONCLUSIONS This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
27647386	4	5	theme	MTT	926:928	arg1	assay					931:935	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	864:935	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	864:935	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	4	6	theme	growth	747:752	arg1	behaviors					769:777	the growth and detachment behaviors	743:777	behaviors	769:777	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	9	7	theme	thermoresponsive	1440:1455	arg1	dishes					1470:1475	thermoresponsive cell culture dishes	1440:1475	thermoresponsive cell culture dishes	1440:1475	CONCLUSIONS This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
27647386	9	8	theme	novel	1540:1544	arg1	material					1546:1553	novel material	1540:1553	novel material applied in tissue engineering	1540:1583	CONCLUSIONS This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
27647386	4	9	theme	TCPS	843:846	arg1	surface					819:825	the surface	815:825	the surface of the modified TCPS	815:846	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	8	10	theme	enzymatic	1342:1350	arg1	digestion					1352:1360	enzymatic digestion	1342:1360	enzymatic digestion	1342:1360	The viability of cells obtained by temperature induction was higher than that obtained by enzymatic digestion.
27647386	9	11	theme	cell	1457:1460	arg1	dishes					1470:1475	thermoresponsive cell culture dishes	1440:1475	thermoresponsive cell culture dishes	1440:1475	CONCLUSIONS This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
27647386	6	12	theme	CS	1131:1132	arg1	introduction					1115:1126	the introduction	1111:1126	the introduction of CS	1111:1132	The cytocompatibility of the materials was improved due to the introduction of CS.
27647386	8	13	theme	temperature	1287:1297	arg1	induction					1299:1307	temperature induction	1287:1307	temperature induction	1287:1307	The viability of cells obtained by temperature induction was higher than that obtained by enzymatic digestion.
27647386	4	14	theme	detachment	758:767	arg1	behaviors					769:777	the growth and detachment behaviors	743:777	behaviors	769:777	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	6	15	theme	materials	1081:1089	arg1	cytocompatibility					1056:1072	The cytocompatibility	1052:1072	The cytocompatibility of the materials	1052:1089	The cytocompatibility of the materials was improved due to the introduction of CS.
27647386	4	16	theme	fibroblast	788:797	arg1	L929					806:809	L929	806:809	L929	806:809	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	4	16	theme	fibroblast	788:797	arg1	cells					799:803	mouse fibroblast cells	782:803	mouse fibroblast cells (L929)	782:810	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	3	17	theme	surface	538:544	arg1	morphology					546:555	surface morphology	538:555	surface morphology	538:555	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	5	18	theme	hydrophobic/hydrophilic	979:1001	arg1	alterations					1012:1022	good hydrophobic/hydrophilic property alterations	974:1022	good hydrophobic/hydrophilic property alterations in response to temperature	974:1049	RESULTS The modified TCPS exhibited good hydrophobic/hydrophilic property alterations in response to temperature.
27647386	1	19	theme	tissue	178:183	arg1	engineering					185:195	tissue engineering	178:195	tissue engineering	178:195	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	2	20	theme	TCPS	457:460	arg1	surface					446:452	the surface	442:452	the surface of TCPS	442:460	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	5	21	theme	good	974:977	arg1	alterations					1012:1022	good hydrophobic/hydrophilic property alterations	974:1022	good hydrophobic/hydrophilic property alterations in response to temperature	974:1049	RESULTS The modified TCPS exhibited good hydrophobic/hydrophilic property alterations in response to temperature.
27647386	2	22	dep	METHODS	336:342	arg1	NIPAAm					384:389	NIPAAm	384:389	NIPAAm	384:389	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	2	22	dep	METHODS	336:342	arg1	N-isopropylacrylamide					361:381	Thermoresponsive N-isopropylacrylamide	344:381	Thermoresponsive N-isopropylacrylamide (NIPAAm)	344:390	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	2	22	dep	METHODS	336:342	arg1	CS					420:421	CS	420:421	CS	420:421	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	2	22	dep	METHODS	336:342	arg1	chitosan					410:417	biocompatible chitosan	396:417	biocompatible chitosan (CS)	396:422	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	2	22	dep	METHODS	336:342	arg1	METHODS					336:342	METHODS	336:342	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS)	336:422	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	3	23	theme	modified	589:596	arg1	TCPS					598:601	the modified TCPS	585:601	the modified TCPS	585:601	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	2	24	theme	biocompatible	396:408	arg1	CS					420:421	CS	420:421	CS	420:421	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	2	24	theme	biocompatible	396:408	arg1	chitosan					410:417	biocompatible chitosan	396:417	biocompatible chitosan (CS)	396:422	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	2	24	theme	biocompatible	396:408	arg1	METHODS					336:342	METHODS	336:342	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS)	336:422	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	1	25	theme	thermoresponsive	284:299	arg1	TCPS					329:332	thermoresponsive tissue culture polystyrene (TCPS)	284:333	thermoresponsive tissue culture polystyrene (TCPS)	284:333	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	9	26	theme	culture	1462:1468	arg1	dishes					1470:1475	thermoresponsive cell culture dishes	1440:1475	thermoresponsive cell culture dishes	1440:1475	CONCLUSIONS This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
27647386	8	27	theme	cells	1269:1273	arg1	higher					1313:1318	higher	1313:1318	higher	1313:1318	The viability of cells obtained by temperature induction was higher than that obtained by enzymatic digestion.
27647386	8	27	theme	cells	1269:1273	arg1	viability					1256:1264	The viability	1252:1264	The viability of cells obtained by temperature induction	1252:1307	The viability of cells obtained by temperature induction was higher than that obtained by enzymatic digestion.
27647386	1	28	theme	tissue	301:306	arg1	TCPS					329:332	thermoresponsive tissue culture polystyrene (TCPS)	284:333	thermoresponsive tissue culture polystyrene (TCPS)	284:333	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	1	29	theme	study	112:116	arg1	aim					100:102	The aim	96:102	The aim of this study	96:116	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	3	30	theme	chemical	516:523	arg1	composition					525:535	The chemical composition	512:535	The chemical composition	512:535	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	0	31	theme	thermoresponsive	47:62	arg1	plates					77:82	chitosan-based thermoresponsive cell culture plates	32:82	chitosan-based thermoresponsive cell culture plates	32:82	Design and cytocompatibility of chitosan-based thermoresponsive cell culture plates.
27647386	1	32	theme	culture	308:314	arg1	TCPS					329:332	thermoresponsive tissue culture polystyrene (TCPS)	284:333	thermoresponsive tissue culture polystyrene (TCPS)	284:333	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	4	33	theme	bromide	917:923	arg1	assay					931:935	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	864:935	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	864:935	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	3	34	theme	atom	664:667	arg1	microscopy					675:684	atom force microscopy	664:684	atom force microscopy (AFM)	664:690	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	3	34	theme	atom	664:667	arg1	AFM					687:689	AFM	687:689	AFM	687:689	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	5	35	theme	property	1003:1010	arg1	alterations					1012:1022	good hydrophobic/hydrophilic property alterations	974:1022	good hydrophobic/hydrophilic property alterations in response to temperature	974:1049	RESULTS The modified TCPS exhibited good hydrophobic/hydrophilic property alterations in response to temperature.
27647386	5	36	from	alterations	1012:1022	arg1	response					1027:1034	response	1027:1034	response to temperature	1027:1049	RESULTS The modified TCPS exhibited good hydrophobic/hydrophilic property alterations in response to temperature.
27647386	0	37	theme	chitosan-based	32:45	arg1	plates					77:82	chitosan-based thermoresponsive cell culture plates	32:82	chitosan-based thermoresponsive cell culture plates	32:82	Design and cytocompatibility of chitosan-based thermoresponsive cell culture plates.
27647386	1	38	theme	polystyrene	316:326	arg1	TCPS					329:332	thermoresponsive tissue culture polystyrene (TCPS)	284:333	thermoresponsive tissue culture polystyrene (TCPS)	284:333	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	4	39	theme	-2,5-diphenyltetrazolium	892:915	arg1	assay					931:935	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	864:935	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	864:935	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	4	40	theme	cells	799:803	arg1	behaviors					769:777	the growth and detachment behaviors	743:777	behaviors	769:777	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	9	41	theme	experimental	1507:1518	arg1	bases					1520:1524	experimental bases	1507:1524	experimental bases	1507:1524	CONCLUSIONS This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
27647386	0	42	theme	culture	69:75	arg1	plates					77:82	chitosan-based thermoresponsive cell culture plates	32:82	chitosan-based thermoresponsive cell culture plates	32:82	Design and cytocompatibility of chitosan-based thermoresponsive cell culture plates.
27647386	0	43	theme	plates	77:82	arg1	cytocompatibility					11:27	cytocompatibility	11:27	cytocompatibility	11:27	Design and cytocompatibility of chitosan-based thermoresponsive cell culture plates.
27647386	0	43	theme	plates	77:82	arg1	Design					0:5	Design	0:5	Design	0:5	Design and cytocompatibility of chitosan-based thermoresponsive cell culture plates.
27647386	3	44	theme	TCPS	598:601	arg1	morphology					546:555	surface morphology	538:555	surface morphology	538:555	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	3	44	theme	TCPS	598:601	arg1	thermoresponsiveness					561:580	thermoresponsiveness	561:580	thermoresponsiveness	561:580	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	3	44	theme	TCPS	598:601	arg1	composition					525:535	The chemical composition	512:535	The chemical composition	512:535	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	1	45	dep	BACKGROUND	85:94	arg1	was					118:120	was	118:120	was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS)	118:333	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	0	46	theme	cell	64:67	arg1	plates					77:82	chitosan-based thermoresponsive cell culture plates	32:82	chitosan-based thermoresponsive cell culture plates	32:82	Design and cytocompatibility of chitosan-based thermoresponsive cell culture plates.
27647386	1	47	theme	TCPS	329:332	arg1	surface					273:279	the surface	269:279	the surface of thermoresponsive tissue culture polystyrene (TCPS)	269:333	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	2	48	theme	Thermoresponsive	344:359	arg1	NIPAAm					384:389	NIPAAm	384:389	NIPAAm	384:389	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	2	48	theme	Thermoresponsive	344:359	arg1	N-isopropylacrylamide					361:381	Thermoresponsive N-isopropylacrylamide	344:381	Thermoresponsive N-isopropylacrylamide (NIPAAm)	344:390	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	2	48	theme	Thermoresponsive	344:359	arg1	METHODS					336:342	METHODS	336:342	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS)	336:422	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	4	49	theme	mouse	782:786	arg1	L929					806:809	L929	806:809	L929	806:809	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	4	49	theme	mouse	782:786	arg1	cells					799:803	mouse fibroblast cells	782:803	mouse fibroblast cells (L929)	782:810	Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
27647386	1	50	dep	growth	217:222	arg1	the					213:215	the	213:215	the	213:215	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	1	50	dep	growth	217:222	arg1	detachment					237:246	detachment	237:246	detachment	237:246	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	9	51	theme	simple	1398:1403	arg1	method					1420:1425	a simple and economical method	1396:1425	a simple and economical method	1396:1425	CONCLUSIONS This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
27647386	5	52	theme	modified	950:957	arg1	TCPS					959:962	The modified TCPS	946:962	The modified TCPS	946:962	RESULTS The modified TCPS exhibited good hydrophobic/hydrophilic property alterations in response to temperature.
27647386	3	53	theme	force	669:673	arg1	microscopy					675:684	atom force microscopy	664:684	atom force microscopy (AFM)	664:690	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	3	53	theme	force	669:673	arg1	AFM					687:689	AFM	687:689	AFM	687:689	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	5	54	dep	RESULTS	938:944	arg1	exhibited					964:972	exhibited	964:972	exhibited good hydrophobic/hydrophilic property alterations in response to temperature	964:1049	RESULTS The modified TCPS exhibited good hydrophobic/hydrophilic property alterations in response to temperature.
27647386	3	55	theme	X-ray	624:628	arg1	XPS					658:660	XPS	658:660	XPS	658:660	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	3	55	theme	X-ray	624:628	arg1	spectroscopy					644:655	X-ray photoelectron spectroscopy	624:655	X-ray photoelectron spectroscopy (XPS)	624:661	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	9	56	theme	new	1490:1492	arg1	thoughts					1494:1501	new thoughts	1490:1501	new thoughts	1490:1501	CONCLUSIONS This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
27647386	1	57	theme	novel	135:139	arg1	material					158:165	a novel thermoresponsive material	133:165	a novel thermoresponsive material suited for tissue engineering	133:195	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	7	58	theme	environmental	1225:1237	arg1	temperature					1239:1249	environmental temperature	1225:1249	environmental temperature	1225:1249	Cells could be spontaneously detached from the surface without any damage, by controlling environmental temperature.
27647386	9	59	theme	economical	1409:1418	arg1	method					1420:1425	a simple and economical method	1396:1425	a simple and economical method	1396:1425	CONCLUSIONS This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
27647386	2	60	theme	graft	490:494	arg1	polymerization					496:509	ultraviolet (UV)-induced graft polymerization	465:509	ultraviolet (UV)-induced graft polymerization	465:509	METHODS Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization.
27647386	3	61	theme	contact	696:702	arg1	CA					711:712	CA	711:712	CA	711:712	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	3	61	theme	contact	696:702	arg1	angle					704:708	contact angle	696:708	contact angle (CA)	696:713	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	3	62	theme	photoelectron	630:642	arg1	XPS					658:660	XPS	658:660	XPS	658:660	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	3	62	theme	photoelectron	630:642	arg1	spectroscopy					644:655	X-ray photoelectron spectroscopy	624:655	X-ray photoelectron spectroscopy (XPS)	624:661	The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively.
27647386	1	63	theme	thermoresponsive	141:156	arg1	material					158:165	a novel thermoresponsive material	133:165	a novel thermoresponsive material suited for tissue engineering	133:195	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	1	64	theme	cells	251:255	arg1	harmless					228:235	harmless	228:235	harmless	228:235	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
27647386	1	64	theme	cells	251:255	arg1	growth					217:222	growth	217:222	growth	217:222	BACKGROUND The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
25800442	1	0	theme	desired	151:157	arg1	activity					173:180	desired antibacterial activity	151:180	desired antibacterial activity	151:180	OBJECTIVE To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
25800442	4	1	theme	aureus	606:611	arg1	suspension					625:634	S. aureus and E. coli suspension	603:634	S. aureus and E. coli suspension for 2 h.	603:643	The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds.
25800442	8	2	theme	concentration	1158:1170	arg1	increase					1137:1144	the increase	1133:1144	the increase of chitosan concentration	1133:1170	The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration.
25800442	6	3	theme	scaffolds	918:926	arg1	properties					900:909	Mechanical properties	889:909	Mechanical properties of KCS scaffolds	889:926	Mechanical properties of KCS scaffolds could be enhanced and well adjusted by chitosan.
25800442	10	4	theme	wound	1421:1425	arg1	healing					1427:1433	wound healing	1421:1433	wound healing	1421:1433	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	3	5	theme	scaffolds	530:538	arg1	composition					456:466	chemical composition	447:466	chemical composition	447:466	The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated.
25800442	3	5	theme	scaffolds	530:538	arg1	ratio					501:505	swelling ratio	492:505	swelling ratio	492:505	The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated.
25800442	3	5	theme	scaffolds	530:538	arg1	wettability					469:479	wettability	469:479	wettability	469:479	The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated.
25800442	3	5	theme	scaffolds	530:538	arg1	degradation					511:521	degradation	511:521	degradation of the scaffolds	511:538	The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated.
25800442	3	5	theme	scaffolds	530:538	arg1	characteristics					430:444	The morphological characteristics	412:444	The morphological characteristics	412:444	The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated.
25800442	3	5	theme	scaffolds	530:538	arg1	porosity					482:489	porosity	482:489	porosity	482:489	The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated.
25800442	1	6	theme	skin	262:265	arg1	regeneration					267:278	skin regeneration	262:278	skin regeneration	262:278	OBJECTIVE To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
25800442	0	7	theme	Wound	99:103	arg1	Healing					105:111	Effectively Accelerating Wound Healing	74:111	Effectively Accelerating Wound Healing	74:111	Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing.
25800442	6	8	theme	KCS	914:916	arg1	scaffolds					918:926	KCS scaffolds	914:926	KCS scaffolds	914:926	Mechanical properties of KCS scaffolds could be enhanced and well adjusted by chitosan.
25800442	1	9	with	dressing	137:144	arg1	ability					206:212	good water maintaining ability	183:212	good water maintaining ability	183:212	OBJECTIVE To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
25800442	1	9	with	dressing	137:144	arg1	properties					229:238	mechanical properties	218:238	mechanical properties	218:238	OBJECTIVE To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
25800442	1	9	with	dressing	137:144	arg1	activity					173:180	desired antibacterial activity	151:180	desired antibacterial activity	151:180	OBJECTIVE To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
25800442	7	10	theme	KCS	977:979	arg1	scaffolds					981:989	KCS scaffolds	977:989	KCS scaffolds	977:989	KCS scaffolds could obviously decrease bacteria number.
25800442	8	11	theme	cells	1065:1069	arg1	proliferation					1037:1049	The proliferation	1033:1049	The proliferation of fibroblast cells in porous KCS	1033:1083	The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration.
25800442	1	12	theme	antibacterial	159:171	arg1	activity					173:180	desired antibacterial activity	151:180	desired antibacterial activity	151:180	OBJECTIVE To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
25800442	0	13	theme	Accelerating	86:97	arg1	Healing					105:111	Effectively Accelerating Wound Healing	74:111	Effectively Accelerating Wound Healing	74:111	Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing.
25800442	8	14	theme	fibroblast	1054:1063	arg1	cells					1065:1069	fibroblast cells	1054:1069	fibroblast cells	1054:1069	The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration.
25800442	9	15	theme	μg/mL	1203:1207	arg1	chitosan					1209:1216	400 μg/mL chitosan	1199:1216	400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples	1199:1325	It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples.
25800442	2	16	theme	porous	371:376	arg1	scaffolds					401:409	porous keratin/chitosan (KCS) scaffolds	371:409	porous keratin/chitosan (KCS) scaffolds	371:409	METHODS The chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds.
25800442	2	16	theme	porous	371:376	arg1	KCS					396:398	KCS	396:398	KCS	396:398	METHODS The chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds.
25800442	4	17	theme	KCS	724:726	arg1	scaffolds					728:736	the KCS scaffolds	720:736	the KCS scaffolds	720:736	The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds.
25800442	10	18	theme	skin	1439:1442	arg1	regeneration					1444:1455	skin regeneration	1439:1455	skin regeneration	1439:1455	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	8	19	theme	porous	1074:1079	arg1	KCS					1081:1083	porous KCS	1074:1083	porous KCS	1074:1083	The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration.
25800442	5	20	theme	degradation	843:853	arg1	rate					855:858	degradation rate	843:858	degradation rate	843:858	RESULTS The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.
25800442	3	21	theme	chemical	447:454	arg1	composition					456:466	chemical composition	447:466	chemical composition	447:466	The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated.
25800442	8	22	dep	increased	1091:1099	arg1	decreased					1118:1126	decreased	1118:1126	increased firstly and then decreased with the increase of chitosan concentration	1091:1170	The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration.
25800442	5	23	theme	adding	751:756	arg1	RESULTS					739:745	RESULTS The adding	739:756	RESULTS The adding of chitosan	739:768	RESULTS The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.
25800442	6	24	theme	Mechanical	889:898	arg1	properties					900:909	Mechanical properties	889:909	Mechanical properties of KCS scaffolds	889:926	Mechanical properties of KCS scaffolds could be enhanced and well adjusted by chitosan.
25800442	4	25	theme	cells	665:669	arg1	culture					671:677	L929 fibroblast cells culture	649:677	L929 fibroblast cells culture	649:677	The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds.
25800442	8	26	theme	chitosan	1149:1156	arg1	concentration					1158:1170	chitosan concentration	1149:1170	chitosan concentration	1149:1170	The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration.
25800442	1	27	theme	good	183:186	arg1	ability					206:212	good water maintaining ability	183:212	good water maintaining ability	183:212	OBJECTIVE To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
25800442	9	28	theme	other	1313:1317	arg1	samples					1319:1325	other samples	1313:1325	other samples	1313:1325	It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples.
25800442	9	29	theme	porous	1226:1231	arg1	scaffold					1237:1244	porous KCS scaffold	1226:1244	porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples	1226:1325	It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples.
25800442	10	30	theme	porous	1343:1348	arg1	materials					1397:1405	implanted scaffold materials	1378:1405	implanted scaffold materials for promoting wound healing and skin regeneration	1378:1455	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	10	30	theme	porous	1343:1348	arg1	scaffold					1354:1361	The porous KCS scaffold	1339:1361	The porous KCS scaffold	1339:1361	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	1	31	theme	water	188:192	arg1	ability					206:212	good water maintaining ability	183:212	good water maintaining ability	183:212	OBJECTIVE To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
25800442	5	32	theme	swelling	824:831	arg1	ratio					833:837	swelling ratio	824:837	swelling ratio	824:837	RESULTS The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.
25800442	9	33	theme	KCS	1233:1235	arg1	scaffold					1237:1244	porous KCS scaffold	1226:1244	porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples	1226:1325	It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples.
25800442	1	34	theme	maintaining	194:204	arg1	ability					206:212	good water maintaining ability	183:212	good water maintaining ability	183:212	OBJECTIVE To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
25800442	10	35	theme	KCS	1350:1352	arg1	materials					1397:1405	implanted scaffold materials	1378:1405	implanted scaffold materials for promoting wound healing and skin regeneration	1378:1455	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	10	35	theme	KCS	1350:1352	arg1	scaffold					1354:1361	The porous KCS scaffold	1339:1361	The porous KCS scaffold	1339:1361	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	5	36	dep	increase	776:783	arg1	decrease					805:812	decrease	805:812	decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds	805:886	RESULTS The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.
25800442	2	37	theme	different	307:315	arg1	concentrations					317:330	different concentrations	307:330	different concentrations	307:330	METHODS The chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds.
25800442	3	38	theme	swelling	492:499	arg1	ratio					501:505	swelling ratio	492:505	swelling ratio	492:505	The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated.
25800442	0	39	theme	Keratin/chitosan	37:52	arg1	Scaffolds					60:68	Porous Keratin/chitosan (KCS) Scaffolds	30:68	Porous Keratin/chitosan (KCS) Scaffolds	30:68	Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing.
25800442	0	39	theme	Keratin/chitosan	37:52	arg1	KCS					55:57	KCS	55:57	KCS	55:57	Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing.
25800442	8	40	from	proliferation	1037:1049	arg1	KCS					1081:1083	porous KCS	1074:1083	porous KCS	1074:1083	The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration.
25800442	5	41	theme	scaffolds	878:886	arg1	rate					855:858	degradation rate	843:858	degradation rate	843:858	RESULTS The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.
25800442	5	41	theme	scaffolds	878:886	arg1	porosity					814:821	porosity	814:821	porosity	814:821	RESULTS The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.
25800442	5	41	theme	scaffolds	878:886	arg1	ratio					833:837	swelling ratio	824:837	swelling ratio	824:837	RESULTS The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.
25800442	4	42	theme	fibroblast	654:663	arg1	cells					665:669	L929 fibroblast cells	649:669	L929 fibroblast cells culture	649:677	The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds.
25800442	0	43	theme	Porous	30:35	arg1	Scaffolds					60:68	Porous Keratin/chitosan (KCS) Scaffolds	30:68	Porous Keratin/chitosan (KCS) Scaffolds	30:68	Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing.
25800442	0	43	theme	Porous	30:35	arg1	KCS					55:57	KCS	55:57	KCS	55:57	Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing.
25800442	5	44	theme	porous	871:876	arg1	scaffolds					878:886	the KCS porous scaffolds	863:886	the KCS porous scaffolds	863:886	RESULTS The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.
25800442	10	45	dep	CONCLUSION	1328:1337	arg1	used					1370:1373	used	1370:1373	may be used as implanted scaffold materials for promoting wound healing and skin regeneration	1363:1455	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	1	46	theme	mechanical	218:227	arg1	properties					229:238	mechanical properties	218:238	mechanical properties	218:238	OBJECTIVE To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
25800442	9	47	theme	best	1260:1263	arg1	attachment					1270:1279	best cell attachment	1260:1279	best cell attachment	1260:1279	It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples.
25800442	4	48	used	used	683:686	arg2	culture					671:677	L929 fibroblast cells culture	649:677	L929 fibroblast cells culture	649:677	The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds.
25800442	3	49	theme	morphological	416:428	arg1	characteristics					430:444	The morphological characteristics	412:444	The morphological characteristics	412:444	The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated.
25800442	9	50	theme	cell	1265:1268	arg1	attachment					1270:1279	best cell attachment	1260:1279	best cell attachment	1260:1279	It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples.
25800442	7	51	theme	bacteria	1016:1023	arg1	number					1025:1030	bacteria number	1016:1030	bacteria number	1016:1030	KCS scaffolds could obviously decrease bacteria number.
25800442	4	52	theme	scaffolds	728:736	arg1	cytotoxicity					704:715	the cytotoxicity	700:715	the cytotoxicity of the KCS scaffolds	700:736	The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds.
25800442	10	53	used	used	1370:1373	arg2	materials					1397:1405	implanted scaffold materials	1378:1405	implanted scaffold materials for promoting wound healing and skin regeneration	1378:1455	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	10	53	used	used	1370:1373	arg2	scaffold					1354:1361	The porous KCS scaffold	1339:1361	The porous KCS scaffold	1339:1361	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	4	54	theme	antibacterial	560:572	arg1	activity					574:581	The antibacterial activity	556:581	The antibacterial activity	556:581	The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds.
25800442	9	55	theme	400	1199:1201	arg1	μg/mL					1203:1207	μg/mL	1203:1207	μg/mL	1203:1207	It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples.
25800442	2	56	theme	keratin	346:352	arg1	solution					354:361	keratin solution	346:361	keratin solution	346:361	METHODS The chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds.
25800442	5	57	dep	RESULTS	739:745	arg1	The					747:749	The	747:749	The	747:749	RESULTS The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.
25800442	10	58	theme	scaffold	1388:1395	arg1	materials					1397:1405	implanted scaffold materials	1378:1405	implanted scaffold materials for promoting wound healing and skin regeneration	1378:1455	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	10	58	theme	scaffold	1388:1395	arg1	scaffold					1354:1361	The porous KCS scaffold	1339:1361	The porous KCS scaffold	1339:1361	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	2	59	theme	keratin/chitosan	378:393	arg1	scaffolds					401:409	porous keratin/chitosan (KCS) scaffolds	371:409	porous keratin/chitosan (KCS) scaffolds	371:409	METHODS The chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds.
25800442	2	59	theme	keratin/chitosan	378:393	arg1	KCS					396:398	KCS	396:398	KCS	396:398	METHODS The chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds.
25800442	4	60	theme	coli	620:623	arg1	suspension					625:634	S. aureus and E. coli suspension	603:634	S. aureus and E. coli suspension for 2 h.	603:643	The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds.
25800442	0	61	theme	Scaffolds	60:68	arg1	Evaluation					16:25	Evaluation	16:25	Evaluation	16:25	Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing.
25800442	0	61	theme	Scaffolds	60:68	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing.
25800442	4	62	theme	L929	649:652	arg1	cells					665:669	L929 fibroblast cells	649:669	L929 fibroblast cells culture	649:677	The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds.
25800442	10	63	theme	implanted	1378:1386	arg1	materials					1397:1405	implanted scaffold materials	1378:1405	implanted scaffold materials for promoting wound healing and skin regeneration	1378:1455	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	10	63	theme	implanted	1378:1386	arg1	scaffold					1354:1361	The porous KCS scaffold	1339:1361	The porous KCS scaffold	1339:1361	CONCLUSION The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
25800442	5	64	theme	KCS	867:869	arg1	scaffolds					878:886	the KCS porous scaffolds	863:886	the KCS porous scaffolds	863:886	RESULTS The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.
25800442	1	65	theme	wound	244:248	arg1	healing					250:256	wound healing	244:256	wound healing	244:256	OBJECTIVE To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
25800442	5	66	theme	chitosan	761:768	arg1	RESULTS					739:745	RESULTS The adding	739:756	RESULTS The adding of chitosan	739:768	RESULTS The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds.
25800442	2	67	dep	METHODS	281:287	arg1	added					337:341	added	337:341	were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds	332:409	METHODS The chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds.
25800442	2	68	with	chitosan	293:300	arg1	concentrations					317:330	different concentrations	307:330	different concentrations	307:330	METHODS The chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds.
28399430	0	0	theme	MAFF	108:111	arg1	lipopolysaccharide					120:137	MAFF 303099 lipopolysaccharide	108:137	MAFF 303099 lipopolysaccharide	108:137	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti MAFF 303099 lipopolysaccharide.
28399430	7	1	theme	-α-l-6dTalp-	1055:1066	arg1	-α-l-Rhap-					1129:1138	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	7	2	theme	M. loti	999:1005	arg1	backbone					1011:1018	the M. loti OPS backbone	995:1018	the M. loti OPS backbone	995:1018	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	4	3	theme	M. loti	684:690	arg1	OPS					710:712	OPS	710:712	OPS	710:712	In this study, we elucidated the chemical structure of M. loti O-polysaccharide (OPS) in PS.
28399430	4	3	theme	M. loti	684:690	arg1	O-polysaccharide					692:707	M. loti O-polysaccharide	684:707	M. loti O-polysaccharide (OPS) in PS	684:719	In this study, we elucidated the chemical structure of M. loti O-polysaccharide (OPS) in PS.
28399430	2	4	theme	transient	431:439	arg1	production					459:468	transient nitric oxide (NO) production	431:468	transient nitric oxide (NO) production in the roots of L. japonicus	431:497	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	3	5	from	part	556:559	arg1	LPS					587:589	LPS	587:589	LPS	587:589	We subsequently found that both the polysaccharide (PS) part and the lipid A moiety in LPS are responsible for the NO induction.
28399430	7	6	theme	-α-l-6dTalp-	1110:1121	arg1	-α-l-Rhap-					1129:1138	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	2	7	theme	lipopolysaccharide	326:343	arg1	preparations					351:362	their lipopolysaccharide (LPS) preparations	320:362	their lipopolysaccharide (LPS) preparations	320:362	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	3	8	theme	lipid	569:573	arg1	responsible					595:605	responsible	595:605	responsible	595:605	We subsequently found that both the polysaccharide (PS) part and the lipid A moiety in LPS are responsible for the NO induction.
28399430	3	8	theme	lipid	569:573	arg1	moiety					577:582	the lipid A moiety	565:582	the lipid A moiety in LPS	565:589	We subsequently found that both the polysaccharide (PS) part and the lipid A moiety in LPS are responsible for the NO induction.
28399430	6	9	theme	deacylated	871:880	arg1	dPS					886:888	dPS	886:888	dPS	886:888	OPS was subjected to hydrazine treatment to obtain deacylated PS (dPS).
28399430	6	9	theme	deacylated	871:880	arg1	PS					882:883	deacylated PS	871:883	deacylated PS (dPS)	871:889	OPS was subjected to hydrazine treatment to obtain deacylated PS (dPS).
28399430	4	10	from	O-polysaccharide	692:707	arg1	PS					718:719	PS	718:719	PS	718:719	In this study, we elucidated the chemical structure of M. loti O-polysaccharide (OPS) in PS.
28399430	7	11	theme	-α-l-6dTalp-	1036:1047	arg1	-α-l-Rhap-					1129:1138	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	7	12	theme	ethylation	923:932	arg1	analysis					934:941	ethylation analysis	923:941	ethylation analysis	923:941	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	0	13	dep	lipopolysaccharide	120:137	arg1	Characterization					0:15	Characterization	0:15	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti	0:106	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti MAFF 303099 lipopolysaccharide.
28399430	1	14	theme	nitrogen-fixing	199:213	arg1	symbioses					215:223	nitrogen-fixing symbioses	199:223	nitrogen-fixing symbioses with several Lotus species	199:250	Mesorhizobium loti is a member of rhizobia and establishes nitrogen-fixing symbioses with several Lotus species.
28399430	7	15	theme	-α-l-Rhap-	1074:1083	arg1	-α-l-Rhap-					1129:1138	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	7	16	theme	composition	901:911	arg1	analysis					913:920	Chemical composition analysis	892:920	Chemical composition analysis	892:920	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	5	17	theme	LPS	773:775	arg1	hydrolysis					751:760	mild acid hydrolysis	741:760	mild acid hydrolysis of M. loti LPS followed by gel filtration chromatography	741:817	PS was prepared by mild acid hydrolysis of M. loti LPS followed by gel filtration chromatography.
28399430	5	18	theme	mild	741:744	arg1	hydrolysis					751:760	mild acid hydrolysis	741:760	mild acid hydrolysis of M. loti LPS followed by gel filtration chromatography	741:817	PS was prepared by mild acid hydrolysis of M. loti LPS followed by gel filtration chromatography.
28399430	7	19	theme	→2	1033:1034	arg1	-α-l-Rhap-					1129:1138	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	0	20	attach	derived	54:60	arg1	loti					103:106	nitric oxide-inducing Mesorhizobium loti	67:106	nitric oxide-inducing Mesorhizobium loti	67:106	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti MAFF 303099 lipopolysaccharide.
28399430	0	20	attach	derived	54:60	arg2	Characterization					0:15	Characterization	0:15	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti	0:106	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti MAFF 303099 lipopolysaccharide.
28399430	3	21	theme	A	575:575	arg1	responsible					595:605	responsible	595:605	responsible	595:605	We subsequently found that both the polysaccharide (PS) part and the lipid A moiety in LPS are responsible for the NO induction.
28399430	3	21	theme	A	575:575	arg1	moiety					577:582	the lipid A moiety	565:582	the lipid A moiety in LPS	565:589	We subsequently found that both the polysaccharide (PS) part and the lipid A moiety in LPS are responsible for the NO induction.
28399430	7	22	theme	-α-l-6dTalp-	1091:1102	arg1	-α-l-Rhap-					1129:1138	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	→2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→	1033:1141	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	4	23	from	PS	718:719	arg1	structure					671:679	the chemical structure	658:679	the chemical structure of M. loti O-polysaccharide (OPS) in PS	658:719	In this study, we elucidated the chemical structure of M. loti O-polysaccharide (OPS) in PS.
28399430	2	24	theme	process	411:417	arg1	beginning					384:392	the beginning	380:392	the beginning of the symbiotic process	380:417	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	0	25	theme	polysaccharide	30:43	arg1	backbone					45:52	O-antigen polysaccharide backbone	20:52	O-antigen polysaccharide backbone	20:52	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti MAFF 303099 lipopolysaccharide.
28399430	7	26	theme	Chemical	892:899	arg1	analysis					913:920	Chemical composition analysis	892:920	Chemical composition analysis	892:920	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	1	27	theme	Lotus	238:242	arg1	species					244:250	several Lotus species	230:250	several Lotus species	230:250	Mesorhizobium loti is a member of rhizobia and establishes nitrogen-fixing symbioses with several Lotus species.
28399430	0	28	theme	O-antigen	20:28	arg1	backbone					45:52	O-antigen polysaccharide backbone	20:52	O-antigen polysaccharide backbone	20:52	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti MAFF 303099 lipopolysaccharide.
28399430	3	29	theme	PS	552:553	arg1	part					556:559	the polysaccharide (PS) part	532:559	the polysaccharide (PS) part	532:559	We subsequently found that both the polysaccharide (PS) part and the lipid A moiety in LPS are responsible for the NO induction.
28399430	3	29	theme	PS	552:553	arg1	responsible					595:605	responsible	595:605	responsible	595:605	We subsequently found that both the polysaccharide (PS) part and the lipid A moiety in LPS are responsible for the NO induction.
28399430	6	30	theme	hydrazine	841:849	arg1	treatment					851:859	hydrazine treatment	841:859	hydrazine treatment	841:859	OPS was subjected to hydrazine treatment to obtain deacylated PS (dPS).
28399430	1	31	theme	Mesorhizobium	140:152	arg1	loti					154:157	Mesorhizobium loti	140:157	Mesorhizobium loti	140:157	Mesorhizobium loti is a member of rhizobia and establishes nitrogen-fixing symbioses with several Lotus species.
28399430	1	31	theme	Mesorhizobium	140:152	arg1	member					164:169	a member	162:169	a member of rhizobia	162:181	Mesorhizobium loti is a member of rhizobia and establishes nitrogen-fixing symbioses with several Lotus species.
28399430	3	32	theme	polysaccharide	536:549	arg1	part					556:559	the polysaccharide (PS) part	532:559	the polysaccharide (PS) part	532:559	We subsequently found that both the polysaccharide (PS) part and the lipid A moiety in LPS are responsible for the NO induction.
28399430	3	32	theme	polysaccharide	536:549	arg1	responsible					595:605	responsible	595:605	responsible	595:605	We subsequently found that both the polysaccharide (PS) part and the lipid A moiety in LPS are responsible for the NO induction.
28399430	5	33	theme	acid	746:749	arg1	hydrolysis					751:760	mild acid hydrolysis	741:760	mild acid hydrolysis of M. loti LPS followed by gel filtration chromatography	741:817	PS was prepared by mild acid hydrolysis of M. loti LPS followed by gel filtration chromatography.
28399430	7	34	dep	-α-l-Rhap-	1129:1138	arg1	1 → 3					1123:1127	1 → 3	1123:1127	1 → 3	1123:1127	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	4	35	from	structure	671:679	arg1	PS					718:719	PS	718:719	PS	718:719	In this study, we elucidated the chemical structure of M. loti O-polysaccharide (OPS) in PS.
28399430	1	36	theme	several	230:236	arg1	species					244:250	several Lotus species	230:250	several Lotus species	230:250	Mesorhizobium loti is a member of rhizobia and establishes nitrogen-fixing symbioses with several Lotus species.
28399430	2	37	theme	oxide	448:452	arg1	production					459:468	transient nitric oxide (NO) production	431:468	transient nitric oxide (NO) production in the roots of L. japonicus	431:497	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	2	38	theme	L. japonicus	486:497	arg1	roots					477:481	the roots	473:481	the roots of L. japonicus	473:497	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	0	39	theme	backbone	45:52	arg1	Characterization					0:15	Characterization	0:15	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti	0:106	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti MAFF 303099 lipopolysaccharide.
28399430	2	40	theme	nitric	441:446	arg1	oxide					448:452	nitric oxide	441:452	transient nitric oxide (NO) production in the roots of L. japonicus	431:497	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	2	40	theme	nitric	441:446	arg1	NO					455:456	NO	455:456	NO	455:456	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	0	41	theme	nitric	67:72	arg1	loti					103:106	nitric oxide-inducing Mesorhizobium loti	67:106	nitric oxide-inducing Mesorhizobium loti	67:106	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti MAFF 303099 lipopolysaccharide.
28399430	7	42	theme	OPS	1007:1009	arg1	backbone					1011:1018	the M. loti OPS backbone	995:1018	the M. loti OPS backbone	995:1018	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	5	43	theme	M. loti	765:771	arg1	LPS					773:775	M. loti LPS	765:775	M. loti LPS	765:775	PS was prepared by mild acid hydrolysis of M. loti LPS followed by gel filtration chromatography.
28399430	7	44	theme	NMR	948:950	arg1	spectra					952:958	NMR spectra	948:958	NMR spectra	948:958	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	7	45	theme	backbone	1011:1018	arg1	structure					982:990	the chemical structure	969:990	the chemical structure of the M. loti OPS backbone in dPS	969:1025	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	7	46	from	structure	982:990	arg1	dPS					1023:1025	dPS	1023:1025	dPS	1023:1025	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	4	47	theme	O-polysaccharide	692:707	arg1	structure					671:679	the chemical structure	658:679	the chemical structure of M. loti O-polysaccharide (OPS) in PS	658:719	In this study, we elucidated the chemical structure of M. loti O-polysaccharide (OPS) in PS.
28399430	3	48	theme	NO	615:616	arg1	induction					618:626	the NO induction	611:626	the NO induction	611:626	We subsequently found that both the polysaccharide (PS) part and the lipid A moiety in LPS are responsible for the NO induction.
28399430	2	49	theme	bacterial	300:308	arg1	cells					310:314	M. loti MAFF 303099 bacterial cells	280:314	M. loti MAFF 303099 bacterial cells	280:314	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	7	50	theme	chemical	973:980	arg1	structure					982:990	the chemical structure	969:990	the chemical structure of the M. loti OPS backbone in dPS	969:1025	Chemical composition analysis, ethylation analysis, and NMR spectra revealed the chemical structure of the M. loti OPS backbone in dPS to be →2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-6dTalp-(1 → 3)-α-l-6dTalp-(1 → 3)-α-l-Rhap-(1→.
28399430	1	51	with	symbioses	215:223	arg1	species					244:250	several Lotus species	230:250	several Lotus species	230:250	Mesorhizobium loti is a member of rhizobia and establishes nitrogen-fixing symbioses with several Lotus species.
28399430	5	52	theme	gel	789:791	arg1	chromatography					804:817	gel filtration chromatography	789:817	gel filtration chromatography	789:817	PS was prepared by mild acid hydrolysis of M. loti LPS followed by gel filtration chromatography.
28399430	4	53	theme	chemical	662:669	arg1	structure					671:679	the chemical structure	658:679	the chemical structure of M. loti O-polysaccharide (OPS) in PS	658:719	In this study, we elucidated the chemical structure of M. loti O-polysaccharide (OPS) in PS.
28399430	0	54	theme	Mesorhizobium	89:101	arg1	loti					103:106	nitric oxide-inducing Mesorhizobium loti	67:106	nitric oxide-inducing Mesorhizobium loti	67:106	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti MAFF 303099 lipopolysaccharide.
28399430	2	55	theme	MAFF	288:291	arg1	cells					310:314	M. loti MAFF 303099 bacterial cells	280:314	M. loti MAFF 303099 bacterial cells	280:314	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	5	56	theme	filtration	793:802	arg1	chromatography					804:817	gel filtration chromatography	789:817	gel filtration chromatography	789:817	PS was prepared by mild acid hydrolysis of M. loti LPS followed by gel filtration chromatography.
28399430	2	57	theme	symbiotic	401:409	arg1	process					411:417	the symbiotic process	397:417	the symbiotic process	397:417	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	3	58	from	moiety	577:582	arg1	LPS					587:589	LPS	587:589	LPS	587:589	We subsequently found that both the polysaccharide (PS) part and the lipid A moiety in LPS are responsible for the NO induction.
28399430	0	59	theme	oxide-inducing	74:87	arg1	loti					103:106	nitric oxide-inducing Mesorhizobium loti	67:106	nitric oxide-inducing Mesorhizobium loti	67:106	Characterization of O-antigen polysaccharide backbone derived from nitric oxide-inducing Mesorhizobium loti MAFF 303099 lipopolysaccharide.
28399430	2	60	from	production	459:468	arg1	roots					477:481	the roots	473:481	the roots of L. japonicus	473:497	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	2	61	theme	M. loti	280:286	arg1	cells					310:314	M. loti MAFF 303099 bacterial cells	280:314	M. loti MAFF 303099 bacterial cells	280:314	Recently, we reported that M. loti MAFF 303099 bacterial cells and their lipopolysaccharide (LPS) preparations are involved in the beginning of the symbiotic process by inducing transient nitric oxide (NO) production in the roots of L. japonicus.
28399430	1	62	theme	rhizobia	174:181	arg1	loti					154:157	Mesorhizobium loti	140:157	Mesorhizobium loti	140:157	Mesorhizobium loti is a member of rhizobia and establishes nitrogen-fixing symbioses with several Lotus species.
28399430	1	62	theme	rhizobia	174:181	arg1	member					164:169	a member	162:169	a member of rhizobia	162:181	Mesorhizobium loti is a member of rhizobia and establishes nitrogen-fixing symbioses with several Lotus species.
28617325	5	0	theme	antioxidant	817:827	arg1	effect					829:834	antioxidant effect	817:834	antioxidant effect on 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activities against Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, and Shigella flexneri	817:1022	The films were also analyzed for antioxidant effect on 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activities against Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, and Shigella flexneri.
28617325	5	1	theme	scavenging	877:886	arg1	activity					888:895	2,2-diphenyl-1-picrylhydrazyl radical scavenging activity	839:895	2,2-diphenyl-1-picrylhydrazyl radical scavenging activity	839:895	The films were also analyzed for antioxidant effect on 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activities against Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, and Shigella flexneri.
28617325	3	2	theme	TA	470:471	arg1	agent					553:557	an antimicrobial agent	536:557	an antimicrobial agent	536:557	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	3	2	theme	TA	470:471	arg1	antioxidant					520:530	antioxidant	520:530	antioxidant	520:530	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	3	2	theme	TA	470:471	arg1	concentrations					444:457	various concentrations	436:457	various concentrations of ASE and TA	436:471	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	1	3	theme	apple	250:254	arg1	ASE					270:272	ASE	270:272	ASE	270:272	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	1	3	theme	apple	250:254	arg1	extract					261:267	apple skin extract (ASE) and tartaric acid (TA)	250:296	extract	261:267	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	0	4	theme	Skin	115:118	arg1	Particles					120:128	Apple Skin Particles	109:128	Apple Skin Particles	109:128	Development of Biopolymer Composite Films Using a Microfluidization Technique for Carboxymethylcellulose and Apple Skin Particles.
28617325	6	5	contain	containing	1060:1069	arg2	ASE					1071:1073	ASE	1071:1073	ASE	1071:1073	From the results, the ASP/CMC film containing ASE and TA was revealed to enhance the mechanical, water barrier, and solubility properties.
28617325	6	5	contain	containing	1060:1069	arg1	film					1055:1058	the ASP/CMC film	1043:1058	the ASP/CMC film containing ASE and TA	1043:1080	From the results, the ASP/CMC film containing ASE and TA was revealed to enhance the mechanical, water barrier, and solubility properties.
28617325	6	5	contain	containing	1060:1069	arg2	TA					1079:1080	TA	1079:1080	TA	1079:1080	From the results, the ASP/CMC film containing ASE and TA was revealed to enhance the mechanical, water barrier, and solubility properties.
28617325	3	6	theme	film	500:503	arg1	solution					505:512	the film solution	496:512	the film solution	496:512	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	5	7	theme	Listeria	934:941	arg1	monocytogenes					943:955	Listeria monocytogenes	934:955	Listeria monocytogenes	934:955	The films were also analyzed for antioxidant effect on 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activities against Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, and Shigella flexneri.
28617325	1	8	theme	skin	256:259	arg1	ASE					270:272	ASE	270:272	ASE	270:272	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	1	8	theme	skin	256:259	arg1	extract					261:267	apple skin extract (ASE) and tartaric acid (TA)	250:296	extract	261:267	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	0	9	theme	Apple	109:113	arg1	Particles					120:128	Apple Skin Particles	109:128	Apple Skin Particles	109:128	Development of Biopolymer Composite Films Using a Microfluidization Technique for Carboxymethylcellulose and Apple Skin Particles.
28617325	7	10	theme	antimicrobial	1215:1227	arg1	properties					1229:1238	the additional antioxidant and antimicrobial properties	1184:1238	the additional antioxidant and antimicrobial properties for application as an active packaging film	1184:1282	Moreover, it showed the additional antioxidant and antimicrobial properties for application as an active packaging film.
28617325	6	11	theme	solubility	1141:1150	arg1	properties					1152:1161	the mechanical, water barrier, and solubility properties	1106:1161	the mechanical, water barrier, and solubility properties	1106:1161	From the results, the ASP/CMC film containing ASE and TA was revealed to enhance the mechanical, water barrier, and solubility properties.
28617325	1	12	theme	extract	261:267	arg1	addition					238:245	the addition	234:245	the addition of apple skin extract (ASE) and tartaric acid (TA)	234:296	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	1	13	theme	Biopolymer	131:140	arg1	films					142:146	Biopolymer films	131:146	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC)	131:212	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	5	14	from	effect	829:834	arg1	activity					888:895	2,2-diphenyl-1-picrylhydrazyl radical scavenging activity	839:895	2,2-diphenyl-1-picrylhydrazyl radical scavenging activity	839:895	The films were also analyzed for antioxidant effect on 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activities against Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, and Shigella flexneri.
28617325	5	14	from	effect	829:834	arg1	activities					915:924	antimicrobial activities	901:924	antimicrobial activities	901:924	The films were also analyzed for antioxidant effect on 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activities against Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, and Shigella flexneri.
28617325	6	15	dep	barrier	1128:1134	arg1	the					1106:1108	the	1106:1108	the	1106:1108	From the results, the ASP/CMC film containing ASE and TA was revealed to enhance the mechanical, water barrier, and solubility properties.
28617325	2	16	theme	ASP	356:358	arg1	solution					360:367	ASP solution	356:367	ASP solution using a microfluidization technique to reduce particle size	356:427	ASP/CMC composite films were prepared by mixing CMC with ASP solution using a microfluidization technique to reduce particle size.
28617325	5	17	theme	2,2-diphenyl-1-picrylhydrazyl	839:867	arg1	activity					888:895	2,2-diphenyl-1-picrylhydrazyl radical scavenging activity	839:895	2,2-diphenyl-1-picrylhydrazyl radical scavenging activity	839:895	The films were also analyzed for antioxidant effect on 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activities against Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, and Shigella flexneri.
28617325	5	18	theme	antimicrobial	901:913	arg1	activities					915:924	antimicrobial activities	901:924	antimicrobial activities	901:924	The films were also analyzed for antioxidant effect on 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activities against Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, and Shigella flexneri.
28617325	2	19	theme	particle	415:422	arg1	size					424:427	particle size	415:427	particle size	415:427	ASP/CMC composite films were prepared by mixing CMC with ASP solution using a microfluidization technique to reduce particle size.
28617325	6	20	theme	barrier	1128:1134	arg1	properties					1152:1161	the mechanical, water barrier, and solubility properties	1106:1161	the mechanical, water barrier, and solubility properties	1106:1161	From the results, the ASP/CMC film containing ASE and TA was revealed to enhance the mechanical, water barrier, and solubility properties.
28617325	4	21	theme	developed	678:686	arg1	films					688:692	the developed films	674:692	the developed films	674:692	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	6	22	theme	ASP/CMC	1047:1053	arg1	film					1055:1058	the ASP/CMC film	1043:1058	the ASP/CMC film containing ASE and TA	1043:1080	From the results, the ASP/CMC film containing ASE and TA was revealed to enhance the mechanical, water barrier, and solubility properties.
28617325	6	23	theme	water	1122:1126	arg1	barrier					1128:1134	water barrier	1122:1134	water barrier	1122:1134	From the results, the ASP/CMC film containing ASE and TA was revealed to enhance the mechanical, water barrier, and solubility properties.
28617325	0	24	theme	Composite	26:34	arg1	Films					36:40	Biopolymer Composite Films	15:40	Biopolymer Composite Films Using a Microfluidization Technique for Carboxymethylcellulose and Apple Skin Particles	15:128	Development of Biopolymer Composite Films Using a Microfluidization Technique for Carboxymethylcellulose and Apple Skin Particles.
28617325	4	25	dep	infrared	592:599	arg1	FTIR					602:605	FTIR	602:605	FTIR	602:605	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	4	25	dep	infrared	592:599	arg1	optical					609:615	optical	609:615	optical	609:615	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	4	25	dep	infrared	592:599	arg1	barrier					636:642	water barrier	630:642	water barrier	630:642	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	4	25	dep	infrared	592:599	arg1	mechanical					618:627	mechanical	618:627	mechanical	618:627	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	2	26	theme	microfluidization	377:393	arg1	technique					395:403	a microfluidization technique	375:403	a microfluidization technique to reduce particle size	375:427	ASP/CMC composite films were prepared by mixing CMC with ASP solution using a microfluidization technique to reduce particle size.
28617325	0	27	theme	Biopolymer	15:24	arg1	Films					36:40	Biopolymer Composite Films	15:40	Biopolymer Composite Films Using a Microfluidization Technique for Carboxymethylcellulose and Apple Skin Particles	15:128	Development of Biopolymer Composite Films Using a Microfluidization Technique for Carboxymethylcellulose and Apple Skin Particles.
28617325	4	28	theme	films	688:692	arg1	properties					660:669	solubility properties	649:669	solubility properties of the developed films	649:692	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	4	29	theme	water	630:634	arg1	barrier					636:642	water barrier	630:642	water barrier	630:642	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	2	30	theme	ASP/CMC	299:305	arg1	films					317:321	ASP/CMC composite films	299:321	ASP/CMC composite films	299:321	ASP/CMC composite films were prepared by mixing CMC with ASP solution using a microfluidization technique to reduce particle size.
28617325	4	31	theme	TA	750:751	arg1	effects					731:737	the effects	727:737	the effects of ASE and TA on physicochemical properties	727:781	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	7	32	theme	packaging	1269:1277	arg1	film					1279:1282	an active packaging film	1259:1282	an active packaging film	1259:1282	Moreover, it showed the additional antioxidant and antimicrobial properties for application as an active packaging film.
28617325	0	33	theme	Films	36:40	arg1	Development					0:10	Development	0:10	Development of Biopolymer Composite Films Using a Microfluidization Technique for Carboxymethylcellulose and Apple Skin Particles.	0:129	Development of Biopolymer Composite Films Using a Microfluidization Technique for Carboxymethylcellulose and Apple Skin Particles.
28617325	1	34	theme	tartaric	279:286	arg1	acid					288:291	tartaric acid	279:291	tartaric acid (TA)	279:296	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	1	34	theme	tartaric	279:286	arg1	TA					294:295	TA	294:295	TA	294:295	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	5	35	theme	Shigella	1006:1013	arg1	flexneri					1015:1022	Shigella flexneri	1006:1022	Shigella flexneri	1006:1022	The films were also analyzed for antioxidant effect on 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activities against Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, and Shigella flexneri.
28617325	0	36	theme	Microfluidization	50:66	arg1	Technique					68:76	a Microfluidization Technique	48:76	a Microfluidization Technique for Carboxymethylcellulose and Apple Skin Particles	48:128	Development of Biopolymer Composite Films Using a Microfluidization Technique for Carboxymethylcellulose and Apple Skin Particles.
28617325	1	37	theme	acid	288:291	arg1	addition					238:245	the addition	234:245	the addition of apple skin extract (ASE) and tartaric acid (TA)	234:296	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	4	38	theme	physicochemical	756:770	arg1	properties					772:781	physicochemical properties	756:781	physicochemical properties	756:781	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	2	39	theme	composite	307:315	arg1	films					317:321	ASP/CMC composite films	299:321	ASP/CMC composite films	299:321	ASP/CMC composite films were prepared by mixing CMC with ASP solution using a microfluidization technique to reduce particle size.
28617325	3	40	theme	antimicrobial	539:551	arg1	agent					553:557	an antimicrobial agent	536:557	an antimicrobial agent	536:557	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	3	40	theme	antimicrobial	539:551	arg1	concentrations					444:457	various concentrations	436:457	various concentrations of ASE and TA	436:471	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	3	41	theme	ASE	462:464	arg1	agent					553:557	an antimicrobial agent	536:557	an antimicrobial agent	536:557	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	3	41	theme	ASE	462:464	arg1	antioxidant					520:530	antioxidant	520:530	antioxidant	520:530	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	3	41	theme	ASE	462:464	arg1	concentrations					444:457	various concentrations	436:457	various concentrations of ASE and TA	436:471	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	4	42	theme	solubility	649:658	arg1	properties					660:669	solubility properties	649:669	solubility properties of the developed films	649:692	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	3	43	theme	various	436:442	arg1	agent					553:557	an antimicrobial agent	536:557	an antimicrobial agent	536:557	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	3	43	theme	various	436:442	arg1	antioxidant					520:530	antioxidant	520:530	antioxidant	520:530	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	3	43	theme	various	436:442	arg1	concentrations					444:457	various concentrations	436:457	various concentrations of ASE and TA	436:471	Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively.
28617325	5	44	theme	radical	869:875	arg1	activity					888:895	2,2-diphenyl-1-picrylhydrazyl radical scavenging activity	839:895	2,2-diphenyl-1-picrylhydrazyl radical scavenging activity	839:895	The films were also analyzed for antioxidant effect on 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activities against Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, and Shigella flexneri.
28617325	7	45	theme	additional	1188:1197	arg1	properties					1229:1238	the additional antioxidant and antimicrobial properties	1184:1238	the additional antioxidant and antimicrobial properties for application as an active packaging film	1184:1282	Moreover, it showed the additional antioxidant and antimicrobial properties for application as an active packaging film.
28617325	4	46	theme	ASE	742:744	arg1	effects					731:737	the effects	727:737	the effects of ASE and TA on physicochemical properties	727:781	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	1	47	theme	apple	157:161	arg1	ASP					176:178	ASP	176:178	ASP	176:178	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	1	47	theme	apple	157:161	arg1	powder					168:173	apple skin powder	157:173	apple skin powder (ASP)	157:179	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	7	48	theme	antioxidant	1199:1209	arg1	properties					1229:1238	the additional antioxidant and antimicrobial properties	1184:1238	the additional antioxidant and antimicrobial properties for application as an active packaging film	1184:1282	Moreover, it showed the additional antioxidant and antimicrobial properties for application as an active packaging film.
28617325	6	49	theme	mechanical	1110:1119	arg1	barrier					1128:1134	water barrier	1122:1134	water barrier	1122:1134	From the results, the ASP/CMC film containing ASE and TA was revealed to enhance the mechanical, water barrier, and solubility properties.
28617325	7	50	theme	active	1262:1267	arg1	film					1279:1282	an active packaging film	1259:1282	an active packaging film	1259:1282	Moreover, it showed the additional antioxidant and antimicrobial properties for application as an active packaging film.
28617325	4	51	from	effects	731:737	arg1	properties					772:781	physicochemical properties	756:781	physicochemical properties	756:781	Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties.
28617325	1	52	theme	skin	163:166	arg1	ASP					176:178	ASP	176:178	ASP	176:178	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
28617325	1	52	theme	skin	163:166	arg1	powder					168:173	apple skin powder	157:173	apple skin powder (ASP)	157:179	Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA).
24273192	0	0	theme	human	85:89	arg1	spine					98:102	the human lumbar spine	81:102	the human lumbar spine	81:102	Influence of biochemical composition on endplate cartilage tensile properties in the human lumbar spine.
24273192	3	1	theme	endplate	359:366	arg1	cartilage					368:376	endplate cartilage	359:376	endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years)	359:442	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	3	2	theme	tensile	539:545	arg1	properties					547:556	its equilibrium tensile properties	523:556	its equilibrium tensile properties	523:556	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	5	3	theme	cartilage	921:929	arg1	status					898:903	the damage status	887:903	the damage status of the adjacent cartilage	887:929	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	6	4	theme	Quasi-linear	1119:1130	arg1	t1					1171:1172	t1	1171:1172	t1	1171:1172	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	4	theme	Quasi-linear	1119:1130	arg1	t2					1180:1181	t2	1180:1181	t2	1180:1181	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	4	theme	Quasi-linear	1119:1130	arg1	C					1168:1168	C	1168:1168	C	1168:1168	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	4	theme	Quasi-linear	1119:1130	arg1	properties					1156:1165	Quasi-linear viscoelastic relaxation properties	1119:1165	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 )	1119:1183	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	0	5	from	Influence	0:8	arg1	endplate					40:47	endplate	40:47	endplate	40:47	Influence of biochemical composition on endplate cartilage tensile properties in the human lumbar spine.
24273192	7	6	theme	biochemical	1372:1382	arg1	composition					1384:1394	biochemical composition	1372:1394	biochemical composition	1372:1394	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	5	7	theme	adjacent	1009:1016	arg1	damage					1018:1023	adjacent damage	1009:1023	adjacent damage	1009:1023	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	6	8	theme	relaxation	1145:1154	arg1	t1					1171:1172	t1	1171:1172	t1	1171:1172	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	8	theme	relaxation	1145:1154	arg1	t2					1180:1181	t2	1180:1181	t2	1180:1181	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	8	theme	relaxation	1145:1154	arg1	C					1168:1168	C	1168:1168	C	1168:1168	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	8	theme	relaxation	1145:1154	arg1	properties					1156:1165	Quasi-linear viscoelastic relaxation properties	1119:1165	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 )	1119:1183	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	0	9	theme	lumbar	91:96	arg1	spine					98:102	the human lumbar spine	81:102	the human lumbar spine	81:102	Influence of biochemical composition on endplate cartilage tensile properties in the human lumbar spine.
24273192	5	10	theme	modulus-collagen/GAG	1073:1092	arg1	p = 0.02					1108:1115	p = 0.02	1108:1115	p = 0.02	1108:1115	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	5	10	theme	modulus-collagen/GAG	1073:1092	arg1	relationship					1094:1105	a diminished modulus-collagen/GAG relationship	1060:1105	a diminished modulus-collagen/GAG relationship (p = 0.02)	1060:1116	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	2	11	theme	biomechanical	311:323	arg1	properties					325:334	biomechanical properties	311:334	biomechanical properties	311:334	Yet, quantitative relationships between endplate biochemical composition and biomechanical properties are unavailable.
24273192	4	12	dep	content	764:770	arg1	r					801:801	r(2) = 0.35	801:811	r(2) = 0.35	801:811	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	4	12	dep	content	764:770	arg1	weight					793:798	559 ± 147 µg/mg dry weight	773:798	559 ± 147 µg/mg dry weight	773:798	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	3	13	theme	endplate	583:590	arg1	damage					592:597	endplate damage	583:597	endplate damage	583:597	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	7	14	theme	matrix	1420:1425	arg1	quantity					1427:1434	matrix quantity	1420:1434	matrix quantity	1420:1434	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	3	15	theme	human	393:397	arg1	spines					406:411	human lumbar spines	393:411	human lumbar spines (six donors, ages 51-67 years)	393:442	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	4	16	theme	dry	789:791	arg1	r					801:801	r(2) = 0.35	801:811	r(2) = 0.35	801:811	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	4	16	theme	dry	789:791	arg1	weight					793:798	559 ± 147 µg/mg dry weight	773:798	559 ± 147 µg/mg dry weight	773:798	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	4	17	theme	equilibrium	681:691	arg1	modulus					701:707	the equilibrium tensile modulus	677:707	the equilibrium tensile modulus (5.9 ± 5.7 MPa)	677:723	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	4	17	theme	equilibrium	681:691	arg1	5.9 ± 5.7 MPa					710:722	5.9 ± 5.7 MPa	710:722	5.9 ± 5.7 MPa	710:722	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	3	18	theme	endplate	459:466	arg1	composition					480:490	endplate biochemical composition	459:490	endplate biochemical composition	459:490	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	3	19	theme	lumbar	399:404	arg1	spines					406:411	human lumbar spines	393:411	human lumbar spines (six donors, ages 51-67 years)	393:442	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	3	20	theme	biochemical	468:478	arg1	composition					480:490	endplate biochemical composition	459:490	endplate biochemical composition	459:490	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	7	21	theme	related	1462:1468	arg1	characteristics					1446:1460	characteristics	1446:1460	characteristics related to matrix quality	1446:1486	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	5	22	theme	latter	944:949	arg1	r					964:964	r(2) = 0.77	964:974	r(2) = 0.77	964:974	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	5	22	theme	latter	944:949	arg1	correlation					951:961	the latter correlation	940:961	the latter correlation (r(2) = 0.77)	940:975	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	7	23	theme	matrix	1473:1478	arg1	quality					1480:1486	matrix quality	1473:1486	matrix quality	1473:1486	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	3	24	theme	ages	426:429	arg1	years					437:441	ages 51-67 years	426:441	ages 51-67 years	426:441	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	3	24	theme	ages	426:429	arg1	donors					418:423	six donors	414:423	six donors	414:423	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	7	25	theme	quantity	1427:1434	arg1	independent					1405:1415	independent	1405:1415	independent	1405:1415	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	7	26	theme	human	1319:1323	arg1	cartilage					1334:1342	human endplate cartilage	1319:1342	human endplate cartilage	1319:1342	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	0	27	theme	composition	25:35	arg1	Influence					0:8	Influence	0:8	Influence of biochemical composition on endplate	0:47	Influence of biochemical composition on endplate cartilage tensile properties in the human lumbar spine.
24273192	7	28	theme	composition	1384:1394	arg1	characteristics					1353:1367	characteristics	1353:1367	characteristics	1353:1367	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	3	29	theme	damage	592:597	arg1	presence					571:578	the presence	567:578	the presence of endplate damage	567:597	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	3	30	dep	showed	447:452	arg1	Using					353:357	Using	353:357	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years)	353:442	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	4	31	theme	tensile	693:699	arg1	modulus					701:707	the equilibrium tensile modulus	677:707	the equilibrium tensile modulus (5.9 ± 5.7 MPa)	677:723	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	4	31	theme	tensile	693:699	arg1	5.9 ± 5.7 MPa					710:722	5.9 ± 5.7 MPa	710:722	5.9 ± 5.7 MPa	710:722	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	0	32	theme	biochemical	13:23	arg1	composition					25:35	biochemical composition	13:35	biochemical composition	13:35	Influence of biochemical composition on endplate cartilage tensile properties in the human lumbar spine.
24273192	3	33	contain	has	492:494	arg2	influence					510:518	a significant influence	496:518	a significant influence	496:518	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	3	33	contain	has	492:494	arg1	composition					480:490	endplate biochemical composition	459:490	endplate biochemical composition	459:490	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	1	34	theme	Endplate	105:112	arg1	integrity					124:132	Endplate cartilage integrity	105:132	Endplate cartilage integrity	105:132	Endplate cartilage integrity is critical to spine health and is presumably impaired by deterioration in biochemical composition.
24273192	3	35	theme	significant	498:508	arg1	influence					510:518	a significant influence	496:518	a significant influence	496:518	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	4	36	theme	559 ± 147 µg/mg	773:787	arg1	r					801:801	r(2) = 0.35	801:811	r(2) = 0.35	801:811	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	4	36	theme	559 ± 147 µg/mg	773:787	arg1	weight					793:798	559 ± 147 µg/mg dry weight	773:798	559 ± 147 µg/mg dry weight	773:798	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	2	37	theme	quantitative	239:250	arg1	relationships					252:264	quantitative relationships	239:264	quantitative relationships between endplate biochemical composition and biomechanical properties	239:334	Yet, quantitative relationships between endplate biochemical composition and biomechanical properties are unavailable.
24273192	1	38	theme	biochemical	209:219	arg1	composition					221:231	biochemical composition	209:231	biochemical composition	209:231	Endplate cartilage integrity is critical to spine health and is presumably impaired by deterioration in biochemical composition.
24273192	6	39	dep	properties	1156:1165	arg1	t1					1171:1172	t1	1171:1172	t1	1171:1172	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	39	dep	properties	1156:1165	arg1	t2					1180:1181	t2	1180:1181	t2	1180:1181	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	39	dep	properties	1156:1165	arg1	C					1168:1168	C	1168:1168	C	1168:1168	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	39	dep	properties	1156:1165	arg1	properties					1156:1165	Quasi-linear viscoelastic relaxation properties	1119:1165	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 )	1119:1183	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	7	40	theme	endplate	1325:1332	arg1	cartilage					1334:1342	human endplate cartilage	1319:1342	human endplate cartilage	1319:1342	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	5	41	contain	had	1056:1058	arg1	fissures					1033:1040	fissures	1033:1040	fissures	1033:1040	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	5	41	contain	had	1056:1058	arg1	avulsions					1046:1054	avulsions	1046:1054	avulsions	1046:1054	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	5	41	contain	had	1056:1058	arg2	p = 0.02					1108:1115	p = 0.02	1108:1115	p = 0.02	1108:1115	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	5	41	contain	had	1056:1058	arg2	relationship					1094:1105	a diminished modulus-collagen/GAG relationship	1060:1105	a diminished modulus-collagen/GAG relationship (p = 0.02)	1060:1116	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	5	41	contain	had	1056:1058	arg1	samples					996:1002	samples	996:1002	samples with adjacent damage such as fissures and avulsions	996:1054	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	7	42	theme	equilibrium	1288:1298	arg1	modulus					1308:1314	the equilibrium tensile modulus	1284:1314	the equilibrium tensile modulus of human endplate cartilage	1284:1342	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	0	43	theme	tensile	59:65	arg1	properties					67:76	tensile properties	59:76	tensile properties	59:76	Influence of biochemical composition on endplate cartilage tensile properties in the human lumbar spine.
24273192	5	44	theme	damage	891:896	arg1	status					898:903	the damage status	887:903	the damage status of the adjacent cartilage	887:929	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	5	45	theme	diminished	1062:1071	arg1	p = 0.02					1108:1115	p = 0.02	1108:1115	p = 0.02	1108:1115	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	5	45	theme	diminished	1062:1071	arg1	relationship					1094:1105	a diminished modulus-collagen/GAG relationship	1060:1105	a diminished modulus-collagen/GAG relationship (p = 0.02)	1060:1116	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	3	46	theme	diminished	617:626	arg1	relationship					649:660	a diminished composition-function relationship	615:660	a diminished composition-function relationship	615:660	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	1	47	theme	cartilage	114:122	arg1	integrity					124:132	Endplate cartilage integrity	105:132	Endplate cartilage integrity	105:132	Endplate cartilage integrity is critical to spine health and is presumably impaired by deterioration in biochemical composition.
24273192	3	48	theme	composition-function	628:647	arg1	relationship					649:660	a diminished composition-function relationship	615:660	a diminished composition-function relationship	615:660	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	7	49	theme	cartilage	1334:1342	arg1	modulus					1308:1314	the equilibrium tensile modulus	1284:1314	the equilibrium tensile modulus of human endplate cartilage	1284:1342	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	3	50	theme	equilibrium	527:537	arg1	properties					547:556	its equilibrium tensile properties	523:556	its equilibrium tensile properties	523:556	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	5	51	with	samples	996:1002	arg1	damage					1018:1023	adjacent damage	1009:1023	adjacent damage	1009:1023	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	3	52	dep	spines	406:411	arg1	years					437:441	ages 51-67 years	426:441	ages 51-67 years	426:441	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	3	52	dep	spines	406:411	arg1	donors					418:423	six donors	414:423	six donors	414:423	Using endplate cartilage harvested from human lumbar spines (six donors, ages 51-67 years) we showed that endplate biochemical composition has a significant influence on its equilibrium tensile properties and that the presence of endplate damage associates with a diminished composition-function relationship.
24273192	4	53	theme	collagen/GAG	827:838	arg1	ratio					840:844	the collagen/GAG ratio	823:844	the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58)	823:869	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	2	54	theme	biochemical	283:293	arg1	composition					295:305	endplate biochemical composition	274:305	endplate biochemical composition	274:305	Yet, quantitative relationships between endplate biochemical composition and biomechanical properties are unavailable.
24273192	6	55	theme	biochemical	1208:1218	arg1	composition					1220:1230	biochemical composition	1208:1230	biochemical composition	1208:1230	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	7	56	theme	reduced	1250:1256	arg1	quantity					1265:1272	reduced matrix quantity	1250:1272	reduced matrix quantity	1250:1272	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	5	57	theme	adjacent	912:919	arg1	cartilage					921:929	the adjacent cartilage	908:929	the adjacent cartilage	908:929	Accounting for the damage status of the adjacent cartilage improved the latter correlation (r(2) = 0.77) and indicated that samples with adjacent damage such as fissures and avulsions had a diminished modulus-collagen/GAG relationship (p = 0.02).
24273192	2	58	theme	endplate	274:281	arg1	composition					295:305	endplate biochemical composition	274:305	endplate biochemical composition	274:305	Yet, quantitative relationships between endplate biochemical composition and biomechanical properties are unavailable.
24273192	7	59	theme	matrix	1258:1263	arg1	quantity					1265:1272	reduced matrix quantity	1250:1272	reduced matrix quantity	1250:1272	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	6	60	theme	viscoelastic	1132:1143	arg1	t1					1171:1172	t1	1171:1172	t1	1171:1172	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	60	theme	viscoelastic	1132:1143	arg1	t2					1180:1181	t2	1180:1181	t2	1180:1181	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	60	theme	viscoelastic	1132:1143	arg1	C					1168:1168	C	1168:1168	C	1168:1168	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	6	60	theme	viscoelastic	1132:1143	arg1	properties					1156:1165	Quasi-linear viscoelastic relaxation properties	1119:1165	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 )	1119:1183	Quasi-linear viscoelastic relaxation properties (C, t1 , and t2 ) did not correlate with biochemical composition.
24273192	7	61	theme	tensile	1300:1306	arg1	modulus					1308:1314	the equilibrium tensile modulus	1284:1314	the equilibrium tensile modulus of human endplate cartilage	1284:1342	We conclude that reduced matrix quantity decreases the equilibrium tensile modulus of human endplate cartilage and that characteristics of biochemical composition that are independent of matrix quantity, that is, characteristics related to matrix quality, may also be important.
24273192	4	62	theme	collagen	755:762	arg1	content					764:770	collagen content	755:770	collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35)	755:812	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	4	63	dep	ratio	840:844	arg1	r					858:858	r(2) = 0.58	858:868	r(2) = 0.58	858:868	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	4	63	dep	ratio	840:844	arg1	6.0 ± 2.1					847:855	6.0 ± 2.1	847:855	6.0 ± 2.1	847:855	We found that the equilibrium tensile modulus (5.9 ± 5.7 MPa) correlated significantly with collagen content (559 ± 147 µg/mg dry weight, r(2) = 0.35) and with the collagen/GAG ratio (6.0 ± 2.1, r(2) = 0.58).
24273192	1	64	theme	spine	149:153	arg1	health					155:160	spine health	149:160	spine health	149:160	Endplate cartilage integrity is critical to spine health and is presumably impaired by deterioration in biochemical composition.
26275030	6	0	theme	drug	1371:1374	arg1	drug					1371:1374	drug	1371:1374	drug	1371:1374	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	6	0	theme	drug	1371:1374	arg1	amounts					1360:1366	higher amounts	1353:1366	higher amounts of drug	1353:1374	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	6	1	theme	Dissolution	1236:1246	arg1	results					1254:1260	Dissolution study results	1236:1260	Dissolution study results	1236:1260	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	1	2	theme	CONTEXT	164:170	arg1	excipients					198:207	the key excipients	190:207	the key excipients administered in tablet formulations	190:243	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	1	2	theme	CONTEXT	164:170	arg1	Disintegrants					172:184	CONTEXT Disintegrants	164:184	CONTEXT Disintegrants	164:184	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	1	3	theme	available	366:374	arg1	area					384:387	the available surface area	362:387	the available surface area	362:387	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	5	4	theme	negative	1190:1197	arg1	tablets					1125:1131	tablets	1125:1131	tablets prepared with sodium starch glycolate (SSG)	1125:1175	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	5	4	theme	negative	1190:1197	arg1	controls					1212:1219	negative and positive controls	1190:1219	negative and positive controls	1190:1219	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	5	4	theme	negative	1190:1197	arg1	RESULTS					1084:1090	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG)	1084:1175	RESULTS	1084:1090	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	7	5	theme	dissolving	1626:1635	arg1	tablets					1637:1643	fast dissolving tablets	1621:1643	fast dissolving tablets	1621:1643	CONCLUSION It was concluded that the novel synthesized superdisintegrant has an appropriate potential for the application in the formulation of fast dissolving tablets.
26275030	1	6	theme	surface	376:382	arg1	area					384:387	the available surface area	362:387	the available surface area	362:387	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	1	7	theme	tablet	279:284	arg1	decomposition					258:270	the decomposition	254:270	the decomposition of the tablet into smaller pieces in the gastrointestinal environment	254:340	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	6	8	theme	higher	1353:1358	arg1	drug					1371:1374	drug	1371:1374	drug	1371:1374	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	6	8	theme	higher	1353:1358	arg1	amounts					1360:1366	higher amounts	1353:1366	higher amounts of drug	1353:1374	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	7	9	contain	has	1550:1552	arg1	superdisintegrant					1532:1548	the novel synthesized superdisintegrant	1510:1548	the novel synthesized superdisintegrant	1510:1548	CONCLUSION It was concluded that the novel synthesized superdisintegrant has an appropriate potential for the application in the formulation of fast dissolving tablets.
26275030	7	9	contain	has	1550:1552	arg2	potential					1569:1577	an appropriate potential	1554:1577	an appropriate potential for the application in the formulation of fast dissolving tablets	1554:1643	CONCLUSION It was concluded that the novel synthesized superdisintegrant has an appropriate potential for the application in the formulation of fast dissolving tablets.
26275030	7	10	from	application	1587:1597	arg1	formulation					1606:1616	the formulation	1602:1616	the formulation of fast dissolving tablets	1602:1643	CONCLUSION It was concluded that the novel synthesized superdisintegrant has an appropriate potential for the application in the formulation of fast dissolving tablets.
26275030	5	11	theme	positive	1203:1210	arg1	tablets					1125:1131	tablets	1125:1131	tablets prepared with sodium starch glycolate (SSG)	1125:1175	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	5	11	theme	positive	1203:1210	arg1	controls					1212:1219	negative and positive controls	1190:1219	negative and positive controls	1190:1219	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	5	11	theme	positive	1203:1210	arg1	RESULTS					1084:1090	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG)	1084:1175	RESULTS	1084:1090	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	4	12	theme	developed	829:837	arg1	superdisintegrant					839:855	the newly developed superdisintegrant	819:855	the newly developed superdisintegrant	819:855	The tablet formulated with the newly developed superdisintegrant was evaluated for hardness, friability, disintegration time, and dissolution rate, and the results were compared with tablets formulated with an identical composition of test formulation differing only in type of disintegrant.
26275030	7	13	theme	tablets	1637:1643	arg1	formulation					1606:1616	the formulation	1602:1616	the formulation of fast dissolving tablets	1602:1643	CONCLUSION It was concluded that the novel synthesized superdisintegrant has an appropriate potential for the application in the formulation of fast dissolving tablets.
26275030	7	14	theme	appropriate	1557:1567	arg1	potential					1569:1577	an appropriate potential	1554:1577	an appropriate potential for the application in the formulation of fast dissolving tablets	1554:1643	CONCLUSION It was concluded that the novel synthesized superdisintegrant has an appropriate potential for the application in the formulation of fast dissolving tablets.
26275030	3	15	dep	nausea	762:767	arg1	medicine					782:789	medicine	782:789	medicine	782:789	METHODS PSI-grafted starch (PSI-St) was synthesized and applied as a disintegrant in the formulation of a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine.
26275030	6	16	theme	study	1248:1252	arg1	results					1254:1260	Dissolution study results	1236:1260	Dissolution study results	1236:1260	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	6	17	attach	released	1381:1388	arg2	drug					1371:1374	drug	1371:1374	drug	1371:1374	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	6	17	attach	released	1381:1388	arg2	amounts					1360:1366	higher amounts	1353:1366	higher amounts of drug	1353:1374	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	6	17	attach	released	1381:1388	arg1	tablets					1395:1401	tablets	1395:1401	tablets formulated from PSI-St than from those formulated from SSG during 10 min	1395:1474	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	6	18	theme	action	1314:1319	arg1	faster					1325:1330	faster	1325:1330	faster	1325:1330	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	6	18	theme	action	1314:1319	arg1	onset					1290:1294	the onset	1286:1294	the onset of disintegration action	1286:1319	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	1	19	theme	key	194:196	arg1	excipients					198:207	the key excipients	190:207	the key excipients administered in tablet formulations	190:243	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	1	19	theme	key	194:196	arg1	Disintegrants					172:184	CONTEXT Disintegrants	164:184	CONTEXT Disintegrants	164:184	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	1	20	theme	smaller	291:297	arg1	pieces					299:304	smaller pieces	291:304	smaller pieces in the gastrointestinal environment	291:340	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	1	21	from	pieces	299:304	arg1	environment					330:340	the gastrointestinal environment	309:340	the gastrointestinal environment	309:340	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	0	22	theme	novel	15:19	arg1	superdisintegrant					21:37	a novel superdisintegrant	13:37	a novel superdisintegrant by starch derivatization with polysuccinimide	13:83	Synthesis of a novel superdisintegrant by starch derivatization with polysuccinimide and its application for the development of Ondansetron fast dissolving tablet.
26275030	3	23	theme	Ondansetron	747:757	arg1	tablet					737:742	a rapidly disintegrating tablet	712:742	a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine	712:789	METHODS PSI-grafted starch (PSI-St) was synthesized and applied as a disintegrant in the formulation of a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine.
26275030	0	24	theme	Ondansetron	128:138	arg1	tablet					156:161	Ondansetron fast dissolving tablet	128:161	Ondansetron fast dissolving tablet	128:161	Synthesis of a novel superdisintegrant by starch derivatization with polysuccinimide and its application for the development of Ondansetron fast dissolving tablet.
26275030	2	25	theme	OBJECTIVE	450:458	arg1	polymer					499:505	a biodegradable polymer	483:505	a biodegradable polymer synthesized from aspartic acid	483:536	OBJECTIVE Polysuccinimide (PSI), a biodegradable polymer synthesized from aspartic acid, was reacted with starch and fully assessed by CHN, 1H-NMR, and FTIR.
26275030	2	25	theme	OBJECTIVE	450:458	arg1	PSI					477:479	PSI	477:479	PSI	477:479	OBJECTIVE Polysuccinimide (PSI), a biodegradable polymer synthesized from aspartic acid, was reacted with starch and fully assessed by CHN, 1H-NMR, and FTIR.
26275030	2	25	theme	OBJECTIVE	450:458	arg1	Polysuccinimide					460:474	OBJECTIVE Polysuccinimide	450:474	OBJECTIVE Polysuccinimide (PSI)	450:480	OBJECTIVE Polysuccinimide (PSI), a biodegradable polymer synthesized from aspartic acid, was reacted with starch and fully assessed by CHN, 1H-NMR, and FTIR.
26275030	3	26	theme	disintegrating	722:735	arg1	tablet					737:742	a rapidly disintegrating tablet	712:742	a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine	712:789	METHODS PSI-grafted starch (PSI-St) was synthesized and applied as a disintegrant in the formulation of a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine.
26275030	4	27	theme	dissolution	922:932	arg1	rate					934:937	dissolution rate	922:937	dissolution rate	922:937	The tablet formulated with the newly developed superdisintegrant was evaluated for hardness, friability, disintegration time, and dissolution rate, and the results were compared with tablets formulated with an identical composition of test formulation differing only in type of disintegrant.
26275030	3	28	dep	METHODS	608:614	arg1	PSI-St					636:641	PSI-St	636:641	PSI-St	636:641	METHODS PSI-grafted starch (PSI-St) was synthesized and applied as a disintegrant in the formulation of a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine.
26275030	3	28	dep	METHODS	608:614	arg1	starch					628:633	PSI-grafted starch	616:633	METHODS PSI-grafted starch (PSI-St)	608:642	METHODS PSI-grafted starch (PSI-St) was synthesized and applied as a disintegrant in the formulation of a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine.
26275030	2	29	theme	biodegradable	485:497	arg1	polymer					499:505	a biodegradable polymer	483:505	a biodegradable polymer synthesized from aspartic acid	483:536	OBJECTIVE Polysuccinimide (PSI), a biodegradable polymer synthesized from aspartic acid, was reacted with starch and fully assessed by CHN, 1H-NMR, and FTIR.
26275030	2	29	theme	biodegradable	485:497	arg1	Polysuccinimide					460:474	OBJECTIVE Polysuccinimide	450:474	OBJECTIVE Polysuccinimide (PSI)	450:480	OBJECTIVE Polysuccinimide (PSI), a biodegradable polymer synthesized from aspartic acid, was reacted with starch and fully assessed by CHN, 1H-NMR, and FTIR.
26275030	3	30	theme	tablet	737:742	arg1	formulation					697:707	the formulation	693:707	the formulation of a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine	693:789	METHODS PSI-grafted starch (PSI-St) was synthesized and applied as a disintegrant in the formulation of a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine.
26275030	0	31	theme	dissolving	145:154	arg1	tablet					156:161	Ondansetron fast dissolving tablet	128:161	Ondansetron fast dissolving tablet	128:161	Synthesis of a novel superdisintegrant by starch derivatization with polysuccinimide and its application for the development of Ondansetron fast dissolving tablet.
26275030	1	32	theme	rapid	410:414	arg1	release					416:422	a more rapid release	403:422	a more rapid release of the active ingredient	403:447	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	4	33	theme	formulation	1032:1042	arg1	composition					1012:1022	an identical composition	999:1022	an identical composition of test formulation	999:1042	The tablet formulated with the newly developed superdisintegrant was evaluated for hardness, friability, disintegration time, and dissolution rate, and the results were compared with tablets formulated with an identical composition of test formulation differing only in type of disintegrant.
26275030	1	34	theme	tablet	225:230	arg1	formulations					232:243	tablet formulations	225:243	tablet formulations	225:243	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	0	35	theme	superdisintegrant	21:37	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of a novel superdisintegrant by starch derivatization with polysuccinimide	0:83	Synthesis of a novel superdisintegrant by starch derivatization with polysuccinimide and its application for the development of Ondansetron fast dissolving tablet.
26275030	0	35	theme	superdisintegrant	21:37	arg1	application					93:103	its application	89:103	its application for the development of Ondansetron fast dissolving tablet	89:161	Synthesis of a novel superdisintegrant by starch derivatization with polysuccinimide and its application for the development of Ondansetron fast dissolving tablet.
26275030	1	36	theme	gastrointestinal	313:328	arg1	environment					330:340	the gastrointestinal environment	309:340	the gastrointestinal environment	309:340	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	0	37	theme	fast	140:143	arg1	tablet					156:161	Ondansetron fast dissolving tablet	128:161	Ondansetron fast dissolving tablet	128:161	Synthesis of a novel superdisintegrant by starch derivatization with polysuccinimide and its application for the development of Ondansetron fast dissolving tablet.
26275030	3	38	from	disintegrant	677:688	arg1	formulation					697:707	the formulation	693:707	the formulation of a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine	693:789	METHODS PSI-grafted starch (PSI-St) was synthesized and applied as a disintegrant in the formulation of a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine.
26275030	1	39	dep	excipients	198:207	arg1	increasing					351:360	increasing	351:360	increasing the available surface area	351:387	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	1	39	dep	excipients	198:207	arg1	enhancing					393:401	enhancing	393:401	enhancing a more rapid release of the active ingredient	393:447	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	4	40	theme	identical	1002:1010	arg1	composition					1012:1022	an identical composition	999:1022	an identical composition of test formulation	999:1042	The tablet formulated with the newly developed superdisintegrant was evaluated for hardness, friability, disintegration time, and dissolution rate, and the results were compared with tablets formulated with an identical composition of test formulation differing only in type of disintegrant.
26275030	5	41	theme	sodium	1147:1152	arg1	glycolate					1161:1169	sodium starch glycolate	1147:1169	sodium starch glycolate (SSG)	1147:1175	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	5	41	theme	sodium	1147:1152	arg1	SSG					1172:1174	SSG	1172:1174	SSG	1172:1174	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	7	42	theme	synthesized	1520:1530	arg1	superdisintegrant					1532:1548	the novel synthesized superdisintegrant	1510:1548	the novel synthesized superdisintegrant	1510:1548	CONCLUSION It was concluded that the novel synthesized superdisintegrant has an appropriate potential for the application in the formulation of fast dissolving tablets.
26275030	0	43	theme	starch	42:47	arg1	derivatization					49:62	starch derivatization	42:62	starch derivatization with polysuccinimide	42:83	Synthesis of a novel superdisintegrant by starch derivatization with polysuccinimide and its application for the development of Ondansetron fast dissolving tablet.
26275030	0	44	theme	tablet	156:161	arg1	development					113:123	the development	109:123	the development of Ondansetron fast dissolving tablet	109:161	Synthesis of a novel superdisintegrant by starch derivatization with polysuccinimide and its application for the development of Ondansetron fast dissolving tablet.
26275030	5	45	used	used	1182:1185	arg2	RESULTS					1084:1090	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG)	1084:1175	RESULTS	1084:1090	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	5	45	used	used	1182:1185	arg2	tablets					1125:1131	tablets	1125:1131	tablets prepared with sodium starch glycolate (SSG)	1125:1175	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	5	45	used	used	1182:1185	arg2	controls					1212:1219	negative and positive controls	1190:1219	negative and positive controls	1190:1219	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	7	46	theme	novel	1514:1518	arg1	superdisintegrant					1532:1548	the novel synthesized superdisintegrant	1510:1548	the novel synthesized superdisintegrant	1510:1548	CONCLUSION It was concluded that the novel synthesized superdisintegrant has an appropriate potential for the application in the formulation of fast dissolving tablets.
26275030	4	47	theme	disintegrant	1070:1081	arg1	type					1062:1065	type	1062:1065	type of disintegrant	1062:1081	The tablet formulated with the newly developed superdisintegrant was evaluated for hardness, friability, disintegration time, and dissolution rate, and the results were compared with tablets formulated with an identical composition of test formulation differing only in type of disintegrant.
26275030	6	48	theme	disintegration	1299:1312	arg1	action					1314:1319	disintegration action	1299:1319	disintegration action	1299:1319	Dissolution study results indicated that although the onset of disintegration action was faster for SSG than PSI-St, higher amounts of drug were released from tablets formulated from PSI-St than from those formulated from SSG during 10 min.
26275030	1	49	theme	active	431:436	arg1	ingredient					438:447	the active ingredient	427:447	the active ingredient	427:447	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	3	50	theme	PSI-grafted	616:626	arg1	PSI-St					636:641	PSI-St	636:641	PSI-St	636:641	METHODS PSI-grafted starch (PSI-St) was synthesized and applied as a disintegrant in the formulation of a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine.
26275030	3	50	theme	PSI-grafted	616:626	arg1	starch					628:633	PSI-grafted starch	616:633	METHODS PSI-grafted starch (PSI-St)	608:642	METHODS PSI-grafted starch (PSI-St) was synthesized and applied as a disintegrant in the formulation of a rapidly disintegrating tablet of Ondansetron, a nausea and vomiting medicine.
26275030	4	51	theme	disintegration	897:910	arg1	time					912:915	disintegration time	897:915	disintegration time	897:915	The tablet formulated with the newly developed superdisintegrant was evaluated for hardness, friability, disintegration time, and dissolution rate, and the results were compared with tablets formulated with an identical composition of test formulation differing only in type of disintegrant.
26275030	1	52	theme	ingredient	438:447	arg1	release					416:422	a more rapid release	403:422	a more rapid release of the active ingredient	403:447	CONTEXT Disintegrants are the key excipients administered in tablet formulations to boost the decomposition of the tablet into smaller pieces in the gastrointestinal environment, thereby increasing the available surface area and enhancing a more rapid release of the active ingredient.
26275030	0	53	with	derivatization	49:62	arg1	polysuccinimide					69:83	polysuccinimide	69:83	polysuccinimide	69:83	Synthesis of a novel superdisintegrant by starch derivatization with polysuccinimide and its application for the development of Ondansetron fast dissolving tablet.
26275030	5	54	dep	RESULTS	1084:1090	arg1	Tablets					1092:1098	Tablets	1092:1098	Tablets prepared with starch	1092:1119	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	2	55	theme	aspartic	524:531	arg1	acid					533:536	aspartic acid	524:536	aspartic acid	524:536	OBJECTIVE Polysuccinimide (PSI), a biodegradable polymer synthesized from aspartic acid, was reacted with starch and fully assessed by CHN, 1H-NMR, and FTIR.
26275030	4	56	theme	test	1027:1030	arg1	formulation					1032:1042	test formulation	1027:1042	test formulation	1027:1042	The tablet formulated with the newly developed superdisintegrant was evaluated for hardness, friability, disintegration time, and dissolution rate, and the results were compared with tablets formulated with an identical composition of test formulation differing only in type of disintegrant.
26275030	7	57	dep	CONCLUSION	1477:1486	arg1	concluded					1495:1503	concluded	1495:1503	was concluded that the novel synthesized superdisintegrant has an appropriate potential for the application in the formulation of fast dissolving tablets	1491:1643	CONCLUSION It was concluded that the novel synthesized superdisintegrant has an appropriate potential for the application in the formulation of fast dissolving tablets.
26275030	7	58	theme	fast	1621:1624	arg1	tablets					1637:1643	fast dissolving tablets	1621:1643	fast dissolving tablets	1621:1643	CONCLUSION It was concluded that the novel synthesized superdisintegrant has an appropriate potential for the application in the formulation of fast dissolving tablets.
26275030	5	59	theme	starch	1154:1159	arg1	glycolate					1161:1169	sodium starch glycolate	1147:1169	sodium starch glycolate (SSG)	1147:1175	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
26275030	5	59	theme	starch	1154:1159	arg1	SSG					1172:1174	SSG	1172:1174	SSG	1172:1174	RESULTS Tablets prepared with starch and tablets prepared with sodium starch glycolate (SSG) were used as negative and positive controls, respectively.
24857815	7	0	theme	pseudoregular	1501:1513	arg1	networks					1525:1532	fibrils to pseudoregular honeycomb networks	1490:1532	fibrils to pseudoregular honeycomb networks	1490:1532	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	6	1	theme	material	1288:1295	arg1	nature					1264:1269	the chemical nature	1251:1269	the chemical nature of the polymeric material used to fabricate the nanomembrane	1251:1330	Type I collagen adsorption largely depends on the substrate employed to support the nanomembrane, whereas it is practically independent of the chemical nature of the polymeric material used to fabricate the nanomembrane.
24857815	6	2	dep	depends	1147:1153	arg1	whereas					1210:1216	whereas	1210:1216	whereas	1210:1216	Type I collagen adsorption largely depends on the substrate employed to support the nanomembrane, whereas it is practically independent of the chemical nature of the polymeric material used to fabricate the nanomembrane.
24857815	1	3	theme	P3TMA	183:187	arg1	derivative					190:199	a polythiophene (P3TMA) derivative	166:199	a polythiophene (P3TMA) derivative	166:199	Nanomembranes have been prepared by spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU) using 20:80, 40:60, and 60:40 TPU:P3TMA weight ratios.
24857815	4	4	theme	hydrolytic	907:916	arg1	medium					918:923	the hydrolytic medium	903:923	the hydrolytic medium	903:923	Moreover, the degradation of the blends is considerably promoted by the presence of enzymes in the hydrolytic medium, TPU:P3TMA blends behaving as biodegradable materials.
24857815	8	5	theme	TPU	1652:1654	arg1	nanomembranes					1662:1674	electroactive TPU:P3TMA nanomembranes	1638:1674	electroactive TPU:P3TMA nanomembranes	1638:1674	Scaffolds made of electroactive TPU:P3TMA nanomembranes are potential candidates for tissue engineering biomedical applications.
24857815	2	6	theme	enzymatic	499:507	arg1	degradation					509:519	both hydrolytic and enzymatic degradation	479:519	both hydrolytic and enzymatic degradation	479:519	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	3	7	theme	hydrolytic	703:712	arg1	degradability					728:740	the hydrolytic and enzymatic degradability	699:740	the hydrolytic and enzymatic degradability of TPU:P3TMA membranes	699:763	The swelling ability and the hydrolytic and enzymatic degradability of TPU:P3TMA membranes increases with the concentration of P3TMA.
24857815	6	8	theme	polymeric	1278:1286	arg1	material					1288:1295	the polymeric material	1274:1295	the polymeric material used to fabricate the nanomembrane	1274:1330	Type I collagen adsorption largely depends on the substrate employed to support the nanomembrane, whereas it is practically independent of the chemical nature of the polymeric material used to fabricate the nanomembrane.
24857815	2	9	theme	thermal	347:353	arg1	characterization					355:370	structural, topographical, electrochemical, and thermal characterization	299:370	structural, topographical, electrochemical, and thermal characterization	299:370	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	8	10	theme	biomedical	1724:1733	arg1	applications					1735:1746	tissue engineering biomedical applications	1705:1746	tissue engineering biomedical applications	1705:1746	Scaffolds made of electroactive TPU:P3TMA nanomembranes are potential candidates for tissue engineering biomedical applications.
24857815	7	11	dep	networks	1525:1532	arg1	to					1498:1499	to	1498:1499	to	1498:1499	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	4	12	theme	TPU	926:928	arg1	blends					936:941	TPU:P3TMA blends	926:941	TPU:P3TMA blends	926:941	Moreover, the degradation of the blends is considerably promoted by the presence of enzymes in the hydrolytic medium, TPU:P3TMA blends behaving as biodegradable materials.
24857815	4	13	from	presence	880:887	arg1	medium					918:923	the hydrolytic medium	903:923	the hydrolytic medium	903:923	Moreover, the degradation of the blends is considerably promoted by the presence of enzymes in the hydrolytic medium, TPU:P3TMA blends behaving as biodegradable materials.
24857815	2	14	theme	matrix	602:607	arg1	protein					609:615	an extra cellular matrix protein	584:615	an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes	584:671	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	14	theme	matrix	602:607	arg1	resistance					465:474	resistance	465:474	resistance to both hydrolytic and enzymatic degradation	465:519	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	14	theme	matrix	602:607	arg1	biocompatibility					522:537	biocompatibility	522:537	biocompatibility	522:537	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	14	theme	matrix	602:607	arg1	adsorption					544:553	adsorption	544:553	adsorption	544:553	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	14	theme	matrix	602:607	arg1	swelling					455:462	swelling	455:462	swelling	455:462	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	7	15	theme	adsorbed	1404:1411	arg1	evidence					1422:1429	adsorbed collagen evidence	1404:1429	adsorbed collagen evidence	1404:1429	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	1	16	theme	TPU	268:270	arg1	ratios					285:290	20:80, 40:60, and 60:40 TPU:P3TMA weight ratios	244:290	20:80, 40:60, and 60:40 TPU:P3TMA weight ratios	244:290	Nanomembranes have been prepared by spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU) using 20:80, 40:60, and 60:40 TPU:P3TMA weight ratios.
24857815	7	17	theme	collagen	1413:1420	arg1	evidence					1422:1429	adsorbed collagen evidence	1404:1429	adsorbed collagen evidence	1404:1429	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	2	18	theme	cellular	593:600	arg1	protein					609:615	an extra cellular matrix protein	584:615	an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes	584:671	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	18	theme	cellular	593:600	arg1	resistance					465:474	resistance	465:474	resistance to both hydrolytic and enzymatic degradation	465:519	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	18	theme	cellular	593:600	arg1	biocompatibility					522:537	biocompatibility	522:537	biocompatibility	522:537	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	18	theme	cellular	593:600	arg1	adsorption					544:553	adsorption	544:553	adsorption	544:553	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	18	theme	cellular	593:600	arg1	swelling					455:462	swelling	455:462	swelling	455:462	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	7	19	theme	different	1448:1456	arg1	organizations					1458:1470	different organizations	1448:1470	different organizations	1448:1470	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	3	20	theme	P3TMA	749:753	arg1	membranes					755:763	TPU:P3TMA membranes	745:763	TPU:P3TMA membranes	745:763	The swelling ability and the hydrolytic and enzymatic degradability of TPU:P3TMA membranes increases with the concentration of P3TMA.
24857815	2	21	theme	cell	649:652	arg1	adhesion					654:661	cell adhesion	649:661	cell adhesion processes	649:671	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	22	theme	adhesion	654:661	arg1	processes					663:671	cell adhesion processes	649:671	cell adhesion processes	649:671	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	7	23	theme	detailed	1342:1349	arg1	microscopy					1351:1360	detailed microscopy	1342:1360	detailed microscopy	1342:1360	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	1	24	theme	derivative	190:199	arg1	mixtures					154:161	spin-coating mixtures	141:161	spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU)	141:236	Nanomembranes have been prepared by spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU) using 20:80, 40:60, and 60:40 TPU:P3TMA weight ratios.
24857815	4	25	theme	biodegradable	955:967	arg1	materials					969:977	biodegradable materials	955:977	biodegradable materials	955:977	Moreover, the degradation of the blends is considerably promoted by the presence of enzymes in the hydrolytic medium, TPU:P3TMA blends behaving as biodegradable materials.
24857815	7	26	theme	organizations	1458:1470	arg1	formation					1435:1443	the formation	1431:1443	the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein	1431:1617	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	6	27	theme	chemical	1255:1262	arg1	nature					1264:1269	the chemical nature	1251:1269	the chemical nature of the polymeric material used to fabricate the nanomembrane	1251:1330	Type I collagen adsorption largely depends on the substrate employed to support the nanomembrane, whereas it is practically independent of the chemical nature of the polymeric material used to fabricate the nanomembrane.
24857815	5	28	theme	other	987:991	arg1	hand					993:996	the other hand	983:996	the other hand	983:996	On the other hand, TPU:P3TMA nanomembranes behave as bioactive platforms stimulating cell adhesion and, especially, cell viability.
24857815	7	29	theme	fibrils	1490:1496	arg1	networks					1525:1532	fibrils to pseudoregular honeycomb networks	1490:1532	fibrils to pseudoregular honeycomb networks	1490:1532	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	0	30	theme	polyurethane	14:25	arg1	polythiophene					27:39	Thermoplastic polyurethane:polythiophene	0:39	Thermoplastic polyurethane:polythiophene	0:39	Thermoplastic polyurethane:polythiophene nanomembranes for biomedical and biotechnological applications.
24857815	3	31	theme	TPU	745:747	arg1	membranes					755:763	TPU:P3TMA membranes	745:763	TPU:P3TMA membranes	745:763	The swelling ability and the hydrolytic and enzymatic degradability of TPU:P3TMA membranes increases with the concentration of P3TMA.
24857815	2	32	theme	topographical	311:323	arg1	characterization					355:370	structural, topographical, electrochemical, and thermal characterization	299:370	structural, topographical, electrochemical, and thermal characterization	299:370	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	5	33	theme	cell	1096:1099	arg1	viability					1101:1109	cell viability	1096:1109	cell viability	1096:1109	On the other hand, TPU:P3TMA nanomembranes behave as bioactive platforms stimulating cell adhesion and, especially, cell viability.
24857815	1	34	theme	thermoplastic	205:217	arg1	TPU					233:235	TPU	233:235	TPU	233:235	Nanomembranes have been prepared by spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU) using 20:80, 40:60, and 60:40 TPU:P3TMA weight ratios.
24857815	1	34	theme	thermoplastic	205:217	arg1	polyurethane					219:230	thermoplastic polyurethane	205:230	thermoplastic polyurethane (TPU)	205:236	Nanomembranes have been prepared by spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU) using 20:80, 40:60, and 60:40 TPU:P3TMA weight ratios.
24857815	0	35	theme	Thermoplastic	0:12	arg1	polythiophene					27:39	Thermoplastic polyurethane:polythiophene	0:39	Thermoplastic polyurethane:polythiophene	0:39	Thermoplastic polyurethane:polythiophene nanomembranes for biomedical and biotechnological applications.
24857815	1	36	theme	P3TMA	272:276	arg1	ratios					285:290	20:80, 40:60, and 60:40 TPU:P3TMA weight ratios	244:290	20:80, 40:60, and 60:40 TPU:P3TMA weight ratios	244:290	Nanomembranes have been prepared by spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU) using 20:80, 40:60, and 60:40 TPU:P3TMA weight ratios.
24857815	6	37	theme	nature	1264:1269	arg1	independent					1236:1246	independent	1236:1246	independent	1236:1246	Type I collagen adsorption largely depends on the substrate employed to support the nanomembrane, whereas it is practically independent of the chemical nature of the polymeric material used to fabricate the nanomembrane.
24857815	1	38	theme	polyurethane	219:230	arg1	mixtures					154:161	spin-coating mixtures	141:161	spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU)	141:236	Nanomembranes have been prepared by spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU) using 20:80, 40:60, and 60:40 TPU:P3TMA weight ratios.
24857815	4	39	dep	promoted	864:871	arg1	behaving					943:950	behaving	943:950	behaving as biodegradable materials	943:977	Moreover, the degradation of the blends is considerably promoted by the presence of enzymes in the hydrolytic medium, TPU:P3TMA blends behaving as biodegradable materials.
24857815	1	40	theme	weight	278:283	arg1	ratios					285:290	20:80, 40:60, and 60:40 TPU:P3TMA weight ratios	244:290	20:80, 40:60, and 60:40 TPU:P3TMA weight ratios	244:290	Nanomembranes have been prepared by spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU) using 20:80, 40:60, and 60:40 TPU:P3TMA weight ratios.
24857815	2	41	theme	structural	299:308	arg1	characterization					355:370	structural, topographical, electrochemical, and thermal characterization	299:370	structural, topographical, electrochemical, and thermal characterization	299:370	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	7	42	theme	evidence	1422:1429	arg1	morphology					1375:1384	morphology	1375:1384	morphology	1375:1384	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	7	42	theme	evidence	1422:1429	arg1	topography					1390:1399	topography	1390:1399	topography	1390:1399	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	3	43	theme	membranes	755:763	arg1	ability					687:693	The swelling ability	674:693	The swelling ability	674:693	The swelling ability and the hydrolytic and enzymatic degradability of TPU:P3TMA membranes increases with the concentration of P3TMA.
24857815	3	43	theme	membranes	755:763	arg1	degradability					728:740	the hydrolytic and enzymatic degradability	699:740	the hydrolytic and enzymatic degradability of TPU:P3TMA membranes	699:763	The swelling ability and the hydrolytic and enzymatic degradability of TPU:P3TMA membranes increases with the concentration of P3TMA.
24857815	2	44	theme	collagen	565:572	arg1	protein					609:615	an extra cellular matrix protein	584:615	an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes	584:671	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	44	theme	collagen	565:572	arg1	resistance					465:474	resistance	465:474	resistance to both hydrolytic and enzymatic degradation	465:519	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	44	theme	collagen	565:572	arg1	adsorption					544:553	adsorption	544:553	adsorption	544:553	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	44	theme	collagen	565:572	arg1	swelling					455:462	swelling	455:462	swelling	455:462	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	44	theme	collagen	565:572	arg1	biocompatibility					522:537	biocompatibility	522:537	biocompatibility	522:537	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	4	45	theme	P3TMA	930:934	arg1	blends					936:941	TPU:P3TMA blends	926:941	TPU:P3TMA blends	926:941	Moreover, the degradation of the blends is considerably promoted by the presence of enzymes in the hydrolytic medium, TPU:P3TMA blends behaving as biodegradable materials.
24857815	2	46	theme	hydrolytic	484:493	arg1	degradation					509:519	both hydrolytic and enzymatic degradation	479:519	both hydrolytic and enzymatic degradation	479:519	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	47	theme	I	563:563	arg1	collagen					565:572	type I collagen	558:572	type I collagen	558:572	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	5	48	theme	P3TMA	1003:1007	arg1	nanomembranes					1009:1021	TPU:P3TMA nanomembranes	999:1021	TPU:P3TMA nanomembranes	999:1021	On the other hand, TPU:P3TMA nanomembranes behave as bioactive platforms stimulating cell adhesion and, especially, cell viability.
24857815	8	49	theme	potential	1680:1688	arg1	candidates					1690:1699	potential candidates	1680:1699	potential candidates for tissue engineering biomedical applications	1680:1746	Scaffolds made of electroactive TPU:P3TMA nanomembranes are potential candidates for tissue engineering biomedical applications.
24857815	8	49	theme	potential	1680:1688	arg1	Scaffolds					1620:1628	Scaffolds	1620:1628	Scaffolds made of electroactive TPU:P3TMA nanomembranes	1620:1674	Scaffolds made of electroactive TPU:P3TMA nanomembranes are potential candidates for tissue engineering biomedical applications.
24857815	7	50	with	contact	1594:1600	arg1	protein					1611:1617	the protein	1607:1617	the protein	1607:1617	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	2	51	theme	biomedical	407:416	arg1	applications					418:429	biomedical applications	407:429	biomedical applications	407:429	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	3	52	theme	P3TMA	801:805	arg1	concentration					784:796	the concentration	780:796	the concentration of P3TMA	780:805	The swelling ability and the hydrolytic and enzymatic degradability of TPU:P3TMA membranes increases with the concentration of P3TMA.
24857815	2	53	theme	type	558:561	arg1	collagen					565:572	type I collagen	558:572	type I collagen	558:572	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	54	theme	extra	587:591	arg1	protein					609:615	an extra cellular matrix protein	584:615	an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes	584:671	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	54	theme	extra	587:591	arg1	resistance					465:474	resistance	465:474	resistance to both hydrolytic and enzymatic degradation	465:519	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	54	theme	extra	587:591	arg1	biocompatibility					522:537	biocompatibility	522:537	biocompatibility	522:537	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	54	theme	extra	587:591	arg1	adsorption					544:553	adsorption	544:553	adsorption	544:553	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	2	54	theme	extra	587:591	arg1	swelling					455:462	swelling	455:462	swelling	455:462	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	7	55	from	networks	1525:1532	arg1	range					1479:1483	range	1479:1483	range	1479:1483	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	8	56	theme	P3TMA	1656:1660	arg1	nanomembranes					1662:1674	electroactive TPU:P3TMA nanomembranes	1638:1674	electroactive TPU:P3TMA nanomembranes	1638:1674	Scaffolds made of electroactive TPU:P3TMA nanomembranes are potential candidates for tissue engineering biomedical applications.
24857815	1	57	theme	spin-coating	141:152	arg1	mixtures					154:161	spin-coating mixtures	141:161	spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU)	141:236	Nanomembranes have been prepared by spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU) using 20:80, 40:60, and 60:40 TPU:P3TMA weight ratios.
24857815	0	58	theme	biomedical	59:68	arg1	applications					91:102	biomedical and biotechnological applications	59:102	applications	91:102	Thermoplastic polyurethane:polythiophene nanomembranes for biomedical and biotechnological applications.
24857815	5	59	theme	bioactive	1033:1041	arg1	platforms					1043:1051	bioactive platforms	1033:1051	bioactive platforms stimulating cell adhesion and, especially, cell viability	1033:1109	On the other hand, TPU:P3TMA nanomembranes behave as bioactive platforms stimulating cell adhesion and, especially, cell viability.
24857815	4	60	theme	blends	841:846	arg1	degradation					822:832	the degradation	818:832	the degradation of the blends	818:846	Moreover, the degradation of the blends is considerably promoted by the presence of enzymes in the hydrolytic medium, TPU:P3TMA blends behaving as biodegradable materials.
24857815	6	61	theme	collagen	1119:1126	arg1	adsorption					1128:1137	Type I collagen adsorption	1112:1137	Type I collagen adsorption	1112:1137	Type I collagen adsorption largely depends on the substrate employed to support the nanomembrane, whereas it is practically independent of the chemical nature of the polymeric material used to fabricate the nanomembrane.
24857815	4	62	attach	presence	880:887	arg1	medium					918:923	the hydrolytic medium	903:923	the hydrolytic medium	903:923	Moreover, the degradation of the blends is considerably promoted by the presence of enzymes in the hydrolytic medium, TPU:P3TMA blends behaving as biodegradable materials.
24857815	4	62	attach	presence	880:887	arg2	enzymes					892:898	enzymes	892:898	enzymes	892:898	Moreover, the degradation of the blends is considerably promoted by the presence of enzymes in the hydrolytic medium, TPU:P3TMA blends behaving as biodegradable materials.
24857815	8	63	theme	electroactive	1638:1650	arg1	nanomembranes					1662:1674	electroactive TPU:P3TMA nanomembranes	1638:1674	electroactive TPU:P3TMA nanomembranes	1638:1674	Scaffolds made of electroactive TPU:P3TMA nanomembranes are potential candidates for tissue engineering biomedical applications.
24857815	7	64	theme	honeycomb	1515:1523	arg1	networks					1525:1532	fibrils to pseudoregular honeycomb networks	1490:1532	fibrils to pseudoregular honeycomb networks	1490:1532	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	2	65	theme	electrochemical	326:340	arg1	characterization					355:370	structural, topographical, electrochemical, and thermal characterization	299:370	structural, topographical, electrochemical, and thermal characterization	299:370	After structural, topographical, electrochemical, and thermal characterization, properties typically related with biomedical applications have been investigated: swelling, resistance to both hydrolytic and enzymatic degradation, biocompatibility, and adsorption of type I collagen, which is an extra cellular matrix protein that binds fibronectin favoring cell adhesion processes.
24857815	3	66	theme	swelling	678:685	arg1	ability					687:693	The swelling ability	674:693	The swelling ability	674:693	The swelling ability and the hydrolytic and enzymatic degradability of TPU:P3TMA membranes increases with the concentration of P3TMA.
24857815	7	67	theme	nanomembrane	1570:1581	arg1	composition					1551:1561	the composition	1547:1561	the composition of the nanomembrane that is in contact with the protein	1547:1617	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	6	68	theme	Type	1112:1115	arg1	collagen					1119:1126	Type I collagen	1112:1126	Type I collagen adsorption	1112:1137	Type I collagen adsorption largely depends on the substrate employed to support the nanomembrane, whereas it is practically independent of the chemical nature of the polymeric material used to fabricate the nanomembrane.
24857815	4	69	theme	enzymes	892:898	arg1	presence					880:887	the presence	876:887	the presence of enzymes in the hydrolytic medium	876:923	Moreover, the degradation of the blends is considerably promoted by the presence of enzymes in the hydrolytic medium, TPU:P3TMA blends behaving as biodegradable materials.
24857815	6	70	theme	I	1117:1117	arg1	collagen					1119:1126	Type I collagen	1112:1126	Type I collagen adsorption	1112:1137	Type I collagen adsorption largely depends on the substrate employed to support the nanomembrane, whereas it is practically independent of the chemical nature of the polymeric material used to fabricate the nanomembrane.
24857815	5	71	theme	cell	1065:1068	arg1	adhesion					1070:1077	cell adhesion	1065:1077	cell adhesion	1065:1077	On the other hand, TPU:P3TMA nanomembranes behave as bioactive platforms stimulating cell adhesion and, especially, cell viability.
24857815	5	72	theme	TPU	999:1001	arg1	nanomembranes					1009:1021	TPU:P3TMA nanomembranes	999:1021	TPU:P3TMA nanomembranes	999:1021	On the other hand, TPU:P3TMA nanomembranes behave as bioactive platforms stimulating cell adhesion and, especially, cell viability.
24857815	1	73	theme	polythiophene	168:180	arg1	derivative					190:199	a polythiophene (P3TMA) derivative	166:199	a polythiophene (P3TMA) derivative	166:199	Nanomembranes have been prepared by spin-coating mixtures of a polythiophene (P3TMA) derivative and thermoplastic polyurethane (TPU) using 20:80, 40:60, and 60:40 TPU:P3TMA weight ratios.
24857815	7	74	dep	morphology	1375:1384	arg1	the					1371:1373	the	1371:1373	the	1371:1373	However, detailed microscopy study of the morphology and topography of adsorbed collagen evidence the formation of different organizations, which range from fibrils to pseudoregular honeycomb networks depending on the composition of the nanomembrane that is in contact with the protein.
24857815	8	75	theme	engineering	1712:1722	arg1	applications					1735:1746	tissue engineering biomedical applications	1705:1746	tissue engineering biomedical applications	1705:1746	Scaffolds made of electroactive TPU:P3TMA nanomembranes are potential candidates for tissue engineering biomedical applications.
24857815	0	76	theme	biotechnological	74:89	arg1	applications					91:102	biomedical and biotechnological applications	59:102	applications	91:102	Thermoplastic polyurethane:polythiophene nanomembranes for biomedical and biotechnological applications.
24857815	3	77	theme	enzymatic	718:726	arg1	degradability					728:740	the hydrolytic and enzymatic degradability	699:740	the hydrolytic and enzymatic degradability of TPU:P3TMA membranes	699:763	The swelling ability and the hydrolytic and enzymatic degradability of TPU:P3TMA membranes increases with the concentration of P3TMA.
24857815	8	78	theme	tissue	1705:1710	arg1	applications					1735:1746	tissue engineering biomedical applications	1705:1746	tissue engineering biomedical applications	1705:1746	Scaffolds made of electroactive TPU:P3TMA nanomembranes are potential candidates for tissue engineering biomedical applications.
25147784	0	0	theme	sea	63:65	arg1	kurodai					80:86	the common sea hare Aplysia kurodai	52:86	the common sea hare Aplysia kurodai	52:86	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.
25147784	6	1	theme	present	856:862	arg1	study					864:868	the present study	852:868	the present study	852:868	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	12	2	theme	other	1740:1744	arg1	mollusks					1746:1753	other mollusks	1740:1753	other mollusks	1740:1753	The sequence of the catalytic domain showed 47-62% amino-acid identities to those of GHF45 cellulases reported in other mollusks.
25147784	6	3	from	4.5	944:946	arg1	temperature					929:939	temperature	929:939	temperature	929:939	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	6	3	from	4.5	944:946	arg1	pH					922:923	optimal pH	914:923	optimal pH	914:923	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	0	4	theme	common	56:61	arg1	kurodai					80:86	the common sea hare Aplysia kurodai	52:86	the common sea hare Aplysia kurodai	52:86	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.
25147784	12	5	theme	amino-acid	1677:1686	arg1	identities					1688:1697	47-62% amino-acid identities	1670:1697	47-62% amino-acid identities	1670:1697	The sequence of the catalytic domain showed 47-62% amino-acid identities to those of GHF45 cellulases reported in other mollusks.
25147784	8	6	theme	end	1172:1174	arg1	cellobiose					1152:1161	cellobiose	1152:1161	cellobiose	1152:1161	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	8	6	theme	end	1172:1174	arg1	cellotriose					1136:1146	cellotriose	1136:1146	cellotriose	1136:1146	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	8	6	theme	end	1172:1174	arg1	products					1176:1183	major end products	1166:1183	major end products but hardly degraded oligosaccharides smaller than tetrasaccharide	1166:1249	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	14	7	theme	fungal	1974:1979	arg1	cellulases					1987:1996	fungal GHF45 cellulases	1974:1996	fungal GHF45 cellulases	1974:1996	Phylogenetic analysis for the amino-acid sequences suggested the close relation between AkEG21 and fungal GHF45 cellulases.
25147784	0	8	theme	Aplysia	72:78	arg1	kurodai					80:86	the common sea hare Aplysia kurodai	52:86	the common sea hare Aplysia kurodai	52:86	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.
25147784	0	9	from	Characterization	0:15	arg1	kurodai					80:86	the common sea hare Aplysia kurodai	52:86	the common sea hare Aplysia kurodai	52:86	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.
25147784	3	10	theme	21	420:421	arg1	kDa					423:425	kDa	423:425	kDa	423:425	The former three cellulases were regarded as glycosyl-hydrolase-family 9 (GHF9) enzymes, while the 21 kDa cellulase was suggested to be a GHF45 enzyme.
25147784	13	11	theme	GHF45	1832:1836	arg1	cellulases					1838:1847	other GHF45 cellulases	1826:1847	other GHF45 cellulases	1826:1847	Both the catalytic residues and the N-glycosylation residues known in other GHF45 cellulases were conserved in AkEG21.
25147784	9	12	theme	endolytic	1285:1293	arg1	AkEG21					1272:1277	AkEG21	1272:1277	AkEG21	1272:1277	This indicated that AkEG21 is an endolytic β-1,4-glucanase (EC 3.2.1.4).
25147784	9	12	theme	endolytic	1285:1293	arg1	EC					1312:1313	EC 3.2.1.4	1312:1321	EC 3.2.1.4	1312:1321	This indicated that AkEG21 is an endolytic β-1,4-glucanase (EC 3.2.1.4).
25147784	9	12	theme	endolytic	1285:1293	arg1	β-1,4-glucanase					1295:1309	an endolytic β-1,4-glucanase	1282:1309	an endolytic β-1,4-glucanase (EC 3.2.1.4)	1282:1322	This indicated that AkEG21 is an endolytic β-1,4-glucanase (EC 3.2.1.4).
25147784	0	13	theme	hare	67:70	arg1	kurodai					80:86	the common sea hare Aplysia kurodai	52:86	the common sea hare Aplysia kurodai	52:86	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.
25147784	13	14	theme	N-glycosylation	1792:1806	arg1	residues					1808:1815	the N-glycosylation residues	1788:1815	the N-glycosylation residues	1788:1815	Both the catalytic residues and the N-glycosylation residues known in other GHF45 cellulases were conserved in AkEG21.
25147784	3	15	theme	kDa	423:425	arg1	enzyme					465:470	a GHF45 enzyme	457:470	a GHF45 enzyme	457:470	The former three cellulases were regarded as glycosyl-hydrolase-family 9 (GHF9) enzymes, while the 21 kDa cellulase was suggested to be a GHF45 enzyme.
25147784	3	15	theme	kDa	423:425	arg1	cellulase					427:435	the 21 kDa cellulase	416:435	the 21 kDa cellulase	416:435	The former three cellulases were regarded as glycosyl-hydrolase-family 9 (GHF9) enzymes, while the 21 kDa cellulase was suggested to be a GHF45 enzyme.
25147784	11	16	theme	signal	1493:1498	arg1	peptide					1500:1506	the putative signal peptide	1480:1506	the putative signal peptide of 16 residues for secretion	1480:1535	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	16	theme	signal	1493:1498	arg1	Met					1475:1477	the initiation Met	1460:1477	the initiation Met	1460:1477	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	5	17	theme	protein	762:768	arg1	study					782:786	the protein engineering study	758:786	the protein engineering study of this cellulase	758:804	In the present study, we determined some enzymatic properties of the 21 kDa cellulase and cloned its cDNA to provide the basis for the protein engineering study of this cellulase.
25147784	12	18	theme	GHF45	1711:1715	arg1	cellulases					1717:1726	GHF45 cellulases	1711:1726	GHF45 cellulases reported in other mollusks	1711:1753	The sequence of the catalytic domain showed 47-62% amino-acid identities to those of GHF45 cellulases reported in other mollusks.
25147784	14	19	theme	GHF45	1981:1985	arg1	cellulases					1987:1996	fungal GHF45 cellulases	1974:1996	fungal GHF45 cellulases	1974:1996	Phylogenetic analysis for the amino-acid sequences suggested the close relation between AkEG21 and fungal GHF45 cellulases.
25147784	14	20	theme	close	1940:1944	arg1	relation					1946:1953	the close relation	1936:1953	the close relation between AkEG21 and fungal GHF45 cellulases	1936:1996	Phylogenetic analysis for the amino-acid sequences suggested the close relation between AkEG21 and fungal GHF45 cellulases.
25147784	11	21	theme	residues	1569:1576	arg1	domain					1555:1560	the catalytic domain	1541:1560	the catalytic domain of 180 residues	1541:1576	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	21	theme	residues	1569:1576	arg1	residues					1569:1576	180 residues	1565:1576	180 residues	1565:1576	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	21	theme	residues	1569:1576	arg1	residues					1514:1521	16 residues	1511:1521	16 residues for secretion	1511:1535	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	21	theme	residues	1569:1576	arg1	peptide					1500:1506	the putative signal peptide	1480:1506	the putative signal peptide of 16 residues for secretion	1480:1535	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	21	theme	residues	1569:1576	arg1	Met					1475:1477	the initiation Met	1460:1477	the initiation Met	1460:1477	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	8	22	theme	degraded	1196:1203	arg1	cellobiose					1152:1161	cellobiose	1152:1161	cellobiose	1152:1161	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	8	22	theme	degraded	1196:1203	arg1	oligosaccharides					1205:1220	degraded oligosaccharides	1196:1220	major end products but hardly degraded oligosaccharides smaller than tetrasaccharide	1166:1249	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	8	22	theme	degraded	1196:1203	arg1	cellotriose					1136:1146	cellotriose	1136:1146	cellotriose	1136:1146	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	3	23	theme	former	325:330	arg1	cellulases					338:347	The former three cellulases	321:347	The former three cellulases	321:347	The former three cellulases were regarded as glycosyl-hydrolase-family 9 (GHF9) enzymes, while the 21 kDa cellulase was suggested to be a GHF45 enzyme.
25147784	3	23	theme	former	325:330	arg1	enzymes					401:407	glycosyl-hydrolase-family 9 (GHF9) enzymes	366:407	glycosyl-hydrolase-family 9 (GHF9) enzymes	366:407	The former three cellulases were regarded as glycosyl-hydrolase-family 9 (GHF9) enzymes, while the 21 kDa cellulase was suggested to be a GHF45 enzyme.
25147784	6	24	theme	purified	811:818	arg1	enzyme					827:832	The purified 21 kDa enzyme	807:832	The purified 21 kDa enzyme	807:832	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	10	25	theme	residues	1415:1422	arg1	sequence					1399:1406	the amino-acid sequence	1384:1406	the amino-acid sequence of 197 residues	1384:1422	A cDNA of 1013 bp encoding AkEG21 was amplified by PCR and the amino-acid sequence of 197 residues was deduced.
25147784	11	26	theme	residues	1514:1521	arg1	domain					1555:1560	the catalytic domain	1541:1560	the catalytic domain of 180 residues	1541:1576	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	26	theme	residues	1514:1521	arg1	residues					1569:1576	180 residues	1565:1576	180 residues	1565:1576	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	26	theme	residues	1514:1521	arg1	residues					1514:1521	16 residues	1511:1521	16 residues for secretion	1511:1535	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	26	theme	residues	1514:1521	arg1	peptide					1500:1506	the putative signal peptide	1480:1506	the putative signal peptide of 16 residues for secretion	1480:1535	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	26	theme	residues	1514:1521	arg1	Met					1475:1477	the initiation Met	1460:1477	the initiation Met	1460:1477	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	6	27	theme	optimal	914:920	arg1	pH					922:923	optimal pH	914:923	optimal pH	914:923	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	11	28	from	N-terminus	1600:1609	arg1	order					1619:1623	this order	1614:1623	this order	1614:1623	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	6	29	from	AkEG21	842:847	arg1	study					864:868	the present study	852:868	the present study	852:868	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	13	30	theme	catalytic	1765:1773	arg1	residues					1775:1782	the catalytic residues	1761:1782	the catalytic residues	1761:1782	Both the catalytic residues and the N-glycosylation residues known in other GHF45 cellulases were conserved in AkEG21.
25147784	4	31	theme	protein-engineering	600:618	arg1	study					620:624	protein-engineering study	600:624	protein-engineering study	600:624	The 21 kDa cellulase was significantly heat stable, and appeared to be advantageous in performing heterogeneous expression and protein-engineering study.
25147784	1	32	theme	good	142:145	arg1	kurodai					117:123	The common sea hare Aplysia kurodai	89:123	The common sea hare Aplysia kurodai	89:123	The common sea hare Aplysia kurodai is known to be a good source for the enzymes degrading seaweed polysaccharides.
25147784	1	32	theme	good	142:145	arg1	source					147:152	a good source	140:152	a good source for the enzymes degrading seaweed polysaccharides	140:202	The common sea hare Aplysia kurodai is known to be a good source for the enzymes degrading seaweed polysaccharides.
25147784	0	33	theme	GHF45	22:26	arg1	AkEG21					39:44	AkEG21	39:44	AkEG21	39:44	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.
25147784	0	33	theme	GHF45	22:26	arg1	cellulase					28:36	a GHF45 cellulase	20:36	a GHF45 cellulase	20:36	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.
25147784	6	34	theme	kDa	823:825	arg1	enzyme					827:832	The purified 21 kDa enzyme	807:832	The purified 21 kDa enzyme	807:832	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	10	35	theme	AkEG21	1352:1357	arg1	cDNA					1327:1330	A cDNA	1325:1330	A cDNA of 1013 bp encoding AkEG21	1325:1357	A cDNA of 1013 bp encoding AkEG21 was amplified by PCR and the amino-acid sequence of 197 residues was deduced.
25147784	12	36	theme	domain	1656:1661	arg1	sequence					1630:1637	The sequence	1626:1637	The sequence of the catalytic domain	1626:1661	The sequence of the catalytic domain showed 47-62% amino-acid identities to those of GHF45 cellulases reported in other mollusks.
25147784	2	37	dep	enzymes	260:266	arg1	i.e.					231:234	i.e.	231:234	i.e.	231:234	Recently four cellulases, i.e., 95, 66, 45, and 21 kDa enzymes, were isolated from A. kurodai (Tsuji et al., 2013).
25147784	5	38	theme	kDa	699:701	arg1	cellulase					703:711	the 21 kDa cellulase	692:711	the 21 kDa cellulase	692:711	In the present study, we determined some enzymatic properties of the 21 kDa cellulase and cloned its cDNA to provide the basis for the protein engineering study of this cellulase.
25147784	5	39	theme	21	696:697	arg1	kDa					699:701	kDa	699:701	kDa	699:701	In the present study, we determined some enzymatic properties of the 21 kDa cellulase and cloned its cDNA to provide the basis for the protein engineering study of this cellulase.
25147784	6	40	theme	21	820:821	arg1	kDa					823:825	kDa	823:825	kDa	823:825	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	5	41	theme	cellulase	796:804	arg1	study					782:786	the protein engineering study	758:786	the protein engineering study of this cellulase	758:804	In the present study, we determined some enzymatic properties of the 21 kDa cellulase and cloned its cDNA to provide the basis for the protein engineering study of this cellulase.
25147784	6	42	dep	pH	922:923	arg1	an					911:912	an	911:912	an	911:912	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	5	43	theme	cellulase	703:711	arg1	properties					678:687	some enzymatic properties	663:687	some enzymatic properties of the 21 kDa cellulase	663:711	In the present study, we determined some enzymatic properties of the 21 kDa cellulase and cloned its cDNA to provide the basis for the protein engineering study of this cellulase.
25147784	2	44	theme	kDa	256:258	arg1	enzymes					260:266	95, 66, 45, and 21 kDa enzymes	237:266	95, 66, 45, and 21 kDa enzymes	237:266	Recently four cellulases, i.e., 95, 66, 45, and 21 kDa enzymes, were isolated from A. kurodai (Tsuji et al., 2013).
25147784	10	45	theme	bp	1340:1341	arg1	AkEG21					1352:1357	1013 bp encoding AkEG21	1335:1357	1013 bp encoding AkEG21	1335:1357	A cDNA of 1013 bp encoding AkEG21 was amplified by PCR and the amino-acid sequence of 197 residues was deduced.
25147784	3	46	theme	GHF45	459:463	arg1	enzyme					465:470	a GHF45 enzyme	457:470	a GHF45 enzyme	457:470	The former three cellulases were regarded as glycosyl-hydrolase-family 9 (GHF9) enzymes, while the 21 kDa cellulase was suggested to be a GHF45 enzyme.
25147784	3	46	theme	GHF45	459:463	arg1	cellulase					427:435	the 21 kDa cellulase	416:435	the 21 kDa cellulase	416:435	The former three cellulases were regarded as glycosyl-hydrolase-family 9 (GHF9) enzymes, while the 21 kDa cellulase was suggested to be a GHF45 enzyme.
25147784	6	47	theme	carboxymethyl	882:894	arg1	cellulose					896:904	carboxymethyl cellulose	882:904	carboxymethyl cellulose	882:904	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	0	48	theme	cellulase	28:36	arg1	Characterization					0:15	Characterization	0:15	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.	0:87	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.
25147784	10	49	theme	1013	1335:1338	arg1	AkEG21					1352:1357	1013 bp encoding AkEG21	1335:1357	1013 bp encoding AkEG21	1335:1357	A cDNA of 1013 bp encoding AkEG21 was amplified by PCR and the amino-acid sequence of 197 residues was deduced.
25147784	5	50	theme	engineering	770:780	arg1	study					782:786	the protein engineering study	758:786	the protein engineering study of this cellulase	758:804	In the present study, we determined some enzymatic properties of the 21 kDa cellulase and cloned its cDNA to provide the basis for the protein engineering study of this cellulase.
25147784	8	51	theme	phosphoric-acid-swollen	1092:1114	arg1	cellulose					1116:1124	phosphoric-acid-swollen cellulose	1092:1124	phosphoric-acid-swollen cellulose	1092:1124	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	10	52	theme	amino-acid	1388:1397	arg1	sequence					1399:1406	the amino-acid sequence	1384:1406	the amino-acid sequence of 197 residues	1384:1422	A cDNA of 1013 bp encoding AkEG21 was amplified by PCR and the amino-acid sequence of 197 residues was deduced.
25147784	6	53	from	40°C	952:955	arg1	temperature					929:939	temperature	929:939	temperature	929:939	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	6	53	from	40°C	952:955	arg1	pH					922:923	optimal pH	914:923	optimal pH	914:923	The purified 21 kDa enzyme, termed AkEG21 in the present study, hydrolyzed carboxymethyl cellulose with an optimal pH and temperature at 4.5 and 40°C, respectively.
25147784	4	54	theme	heterogeneous	571:583	arg1	expression					585:594	heterogeneous expression	571:594	heterogeneous expression	571:594	The 21 kDa cellulase was significantly heat stable, and appeared to be advantageous in performing heterogeneous expression and protein-engineering study.
25147784	5	55	theme	enzymatic	668:676	arg1	properties					678:687	some enzymatic properties	663:687	some enzymatic properties of the 21 kDa cellulase	663:711	In the present study, we determined some enzymatic properties of the 21 kDa cellulase and cloned its cDNA to provide the basis for the protein engineering study of this cellulase.
25147784	11	56	theme	putative	1484:1491	arg1	peptide					1500:1506	the putative signal peptide	1480:1506	the putative signal peptide of 16 residues for secretion	1480:1535	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	56	theme	putative	1484:1491	arg1	Met					1475:1477	the initiation Met	1460:1477	the initiation Met	1460:1477	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	12	57	theme	catalytic	1646:1654	arg1	domain					1656:1661	the catalytic domain	1642:1661	the catalytic domain	1642:1661	The sequence of the catalytic domain showed 47-62% amino-acid identities to those of GHF45 cellulases reported in other mollusks.
25147784	1	58	theme	common	93:98	arg1	kurodai					117:123	The common sea hare Aplysia kurodai	89:123	The common sea hare Aplysia kurodai	89:123	The common sea hare Aplysia kurodai is known to be a good source for the enzymes degrading seaweed polysaccharides.
25147784	1	58	theme	common	93:98	arg1	source					147:152	a good source	140:152	a good source for the enzymes degrading seaweed polysaccharides	140:202	The common sea hare Aplysia kurodai is known to be a good source for the enzymes degrading seaweed polysaccharides.
25147784	12	59	theme	%	1675:1675	arg1	identities					1688:1697	47-62% amino-acid identities	1670:1697	47-62% amino-acid identities	1670:1697	The sequence of the catalytic domain showed 47-62% amino-acid identities to those of GHF45 cellulases reported in other mollusks.
25147784	0	60	from	kurodai	80:86	arg1	Characterization					0:15	Characterization	0:15	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.	0:87	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.
25147784	0	60	from	kurodai	80:86	arg1	AkEG21					39:44	AkEG21	39:44	AkEG21	39:44	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.
25147784	0	60	from	kurodai	80:86	arg1	cellulase					28:36	a GHF45 cellulase	20:36	a GHF45 cellulase	20:36	Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai.
25147784	2	61	dep	kurodai	291:297	arg1	Tsuji					300:304	Tsuji	300:304	Tsuji	300:304	Recently four cellulases, i.e., 95, 66, 45, and 21 kDa enzymes, were isolated from A. kurodai (Tsuji et al., 2013).
25147784	2	61	dep	kurodai	291:297	arg1	2013					314:317	2013	314:317	2013	314:317	Recently four cellulases, i.e., 95, 66, 45, and 21 kDa enzymes, were isolated from A. kurodai (Tsuji et al., 2013).
25147784	1	62	theme	sea	100:102	arg1	kurodai					117:123	The common sea hare Aplysia kurodai	89:123	The common sea hare Aplysia kurodai	89:123	The common sea hare Aplysia kurodai is known to be a good source for the enzymes degrading seaweed polysaccharides.
25147784	1	62	theme	sea	100:102	arg1	source					147:152	a good source	140:152	a good source for the enzymes degrading seaweed polysaccharides	140:202	The common sea hare Aplysia kurodai is known to be a good source for the enzymes degrading seaweed polysaccharides.
25147784	14	63	theme	Phylogenetic	1875:1886	arg1	analysis					1888:1895	Phylogenetic analysis	1875:1895	Phylogenetic analysis for the amino-acid sequences	1875:1924	Phylogenetic analysis for the amino-acid sequences suggested the close relation between AkEG21 and fungal GHF45 cellulases.
25147784	12	64	theme	47-62	1670:1674	arg1	%					1675:1675	%	1675:1675	%	1675:1675	The sequence of the catalytic domain showed 47-62% amino-acid identities to those of GHF45 cellulases reported in other mollusks.
25147784	11	65	theme	catalytic	1545:1553	arg1	domain					1555:1560	the catalytic domain	1541:1560	the catalytic domain of 180 residues	1541:1576	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	65	theme	catalytic	1545:1553	arg1	residues					1569:1576	180 residues	1565:1576	180 residues	1565:1576	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	65	theme	catalytic	1545:1553	arg1	residues					1514:1521	16 residues	1511:1521	16 residues for secretion	1511:1535	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	65	theme	catalytic	1545:1553	arg1	Met					1475:1477	the initiation Met	1460:1477	the initiation Met	1460:1477	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	8	66	theme	major	1166:1170	arg1	cellobiose					1152:1161	cellobiose	1152:1161	cellobiose	1152:1161	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	8	66	theme	major	1166:1170	arg1	cellotriose					1136:1146	cellotriose	1136:1146	cellotriose	1136:1146	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	8	66	theme	major	1166:1170	arg1	products					1176:1183	major end products	1166:1183	major end products but hardly degraded oligosaccharides smaller than tetrasaccharide	1166:1249	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	11	67	theme	initiation	1464:1473	arg1	domain					1555:1560	the catalytic domain	1541:1560	the catalytic domain of 180 residues	1541:1576	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	67	theme	initiation	1464:1473	arg1	residues					1569:1576	180 residues	1565:1576	180 residues	1565:1576	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	67	theme	initiation	1464:1473	arg1	residues					1514:1521	16 residues	1511:1521	16 residues for secretion	1511:1535	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	67	theme	initiation	1464:1473	arg1	peptide					1500:1506	the putative signal peptide	1480:1506	the putative signal peptide of 16 residues for secretion	1480:1535	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	11	67	theme	initiation	1464:1473	arg1	Met					1475:1477	the initiation Met	1460:1477	the initiation Met	1460:1477	The sequence comprised the initiation Met, the putative signal peptide of 16 residues for secretion and the catalytic domain of 180 residues, which lined from the N-terminus in this order.
25147784	1	68	theme	hare	104:107	arg1	kurodai					117:123	The common sea hare Aplysia kurodai	89:123	The common sea hare Aplysia kurodai	89:123	The common sea hare Aplysia kurodai is known to be a good source for the enzymes degrading seaweed polysaccharides.
25147784	1	68	theme	hare	104:107	arg1	source					147:152	a good source	140:152	a good source for the enzymes degrading seaweed polysaccharides	140:202	The common sea hare Aplysia kurodai is known to be a good source for the enzymes degrading seaweed polysaccharides.
25147784	8	69	theme	smaller	1222:1228	arg1	cellobiose					1152:1161	cellobiose	1152:1161	cellobiose	1152:1161	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	8	69	theme	smaller	1222:1228	arg1	oligosaccharides					1205:1220	degraded oligosaccharides	1196:1220	major end products but hardly degraded oligosaccharides smaller than tetrasaccharide	1166:1249	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	8	69	theme	smaller	1222:1228	arg1	cellotriose					1136:1146	cellotriose	1136:1146	cellotriose	1136:1146	AkEG21 degraded phosphoric-acid-swollen cellulose producing cellotriose and cellobiose as major end products but hardly degraded oligosaccharides smaller than tetrasaccharide.
25147784	1	70	theme	seaweed	180:186	arg1	polysaccharides					188:202	seaweed polysaccharides	180:202	seaweed polysaccharides	180:202	The common sea hare Aplysia kurodai is known to be a good source for the enzymes degrading seaweed polysaccharides.
25147784	10	71	theme	encoding	1343:1350	arg1	AkEG21					1352:1357	1013 bp encoding AkEG21	1335:1357	1013 bp encoding AkEG21	1335:1357	A cDNA of 1013 bp encoding AkEG21 was amplified by PCR and the amino-acid sequence of 197 residues was deduced.
25147784	7	72	from	55°C	1059:1062	arg1	incubation					1045:1054	the incubation	1041:1054	the incubation at 55°C for 30 min	1041:1073	AkEG21 was considerably heat-stable, i.e., it was not inactivated by the incubation at 55°C for 30 min.
25147784	2	73	dep	Tsuji	300:304	arg1	al.					309:311	Tsuji et al.	300:311	Tsuji et al.	300:311	Recently four cellulases, i.e., 95, 66, 45, and 21 kDa enzymes, were isolated from A. kurodai (Tsuji et al., 2013).
25147784	1	74	theme	Aplysia	109:115	arg1	kurodai					117:123	The common sea hare Aplysia kurodai	89:123	The common sea hare Aplysia kurodai	89:123	The common sea hare Aplysia kurodai is known to be a good source for the enzymes degrading seaweed polysaccharides.
25147784	1	74	theme	Aplysia	109:115	arg1	source					147:152	a good source	140:152	a good source for the enzymes degrading seaweed polysaccharides	140:202	The common sea hare Aplysia kurodai is known to be a good source for the enzymes degrading seaweed polysaccharides.
25147784	14	75	theme	amino-acid	1905:1914	arg1	sequences					1916:1924	the amino-acid sequences	1901:1924	the amino-acid sequences	1901:1924	Phylogenetic analysis for the amino-acid sequences suggested the close relation between AkEG21 and fungal GHF45 cellulases.
25147784	4	76	theme	kDa	480:482	arg1	advantageous					544:555	advantageous	544:555	advantageous	544:555	The 21 kDa cellulase was significantly heat stable, and appeared to be advantageous in performing heterogeneous expression and protein-engineering study.
25147784	4	76	theme	kDa	480:482	arg1	cellulase					484:492	The 21 kDa cellulase	473:492	The 21 kDa cellulase	473:492	The 21 kDa cellulase was significantly heat stable, and appeared to be advantageous in performing heterogeneous expression and protein-engineering study.
25147784	4	76	theme	kDa	480:482	arg1	stable					517:522	stable	517:522	stable	517:522	The 21 kDa cellulase was significantly heat stable, and appeared to be advantageous in performing heterogeneous expression and protein-engineering study.
25147784	5	77	theme	present	634:640	arg1	study					642:646	the present study	630:646	the present study	630:646	In the present study, we determined some enzymatic properties of the 21 kDa cellulase and cloned its cDNA to provide the basis for the protein engineering study of this cellulase.
25147784	2	78	attach	isolated	274:281	arg2	cellulases					219:228	four cellulases	214:228	four cellulases	214:228	Recently four cellulases, i.e., 95, 66, 45, and 21 kDa enzymes, were isolated from A. kurodai (Tsuji et al., 2013).
25147784	2	78	attach	isolated	274:281	arg1	kurodai					291:297	kurodai	291:297	kurodai	291:297	Recently four cellulases, i.e., 95, 66, 45, and 21 kDa enzymes, were isolated from A. kurodai (Tsuji et al., 2013).
25147784	13	79	theme	other	1826:1830	arg1	cellulases					1838:1847	other GHF45 cellulases	1826:1847	other GHF45 cellulases	1826:1847	Both the catalytic residues and the N-glycosylation residues known in other GHF45 cellulases were conserved in AkEG21.
25147784	4	80	theme	21	477:478	arg1	kDa					480:482	kDa	480:482	kDa	480:482	The 21 kDa cellulase was significantly heat stable, and appeared to be advantageous in performing heterogeneous expression and protein-engineering study.
27083835	8	0	theme	low	1265:1267	arg1	molecules					1286:1294	low molecular weight molecules	1265:1294	low molecular weight molecules from macro and micro-capsules	1265:1324	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	8	1	from	micro-capsules	1311:1324	arg1	molecules					1286:1294	low molecular weight molecules	1265:1294	low molecular weight molecules from macro and micro-capsules	1265:1324	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	8	1	from	micro-capsules	1311:1324	arg1	mechanism					1252:1260	control release mechanism	1236:1260	control release mechanism of low molecular weight molecules from macro and micro-capsules	1236:1324	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	5	2	theme	electron	697:704	arg1	microscopy					706:715	Optical, fluorescence and scanning electron microscopy	662:715	microscopy	706:715	Optical, fluorescence and scanning electron microscopy, highlighted different capsules and membranes architectures.
27083835	7	3	theme	structural	1084:1093	arg1	change					1095:1100	irreversible structural change	1071:1100	irreversible structural change due to thermoplastic behavior of shellac	1071:1141	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	1	4	theme	capsules	192:199	arg1	properties					157:166	physico-chemical properties	140:166	physico-chemical properties of alginate liquid-core capsules	140:199	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	0	5	theme	architecture	93:104	arg1	Influence					71:79	Influence	71:79	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.	0:127	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.
27083835	6	6	theme	vitamin	887:893	arg1	release					862:868	a pH-dependent release	847:868	a pH-dependent release of the entrapped vitamin	847:893	In contrast to simple and composite capsules, coated capsules showed a pH-dependent release of the entrapped vitamin especially after shellac crosslinking with calcium.
27083835	1	7	theme	additional	275:284	arg1	capsules					309:316	coated capsules	302:316	coated capsules	302:316	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	1	7	theme	additional	275:284	arg1	shellac					202:208	shellac	202:208	shellac	202:208	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	1	7	theme	additional	275:284	arg1	layer					295:299	an additional external layer	272:299	an additional external layer (coated capsules)	272:317	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	8	8	theme	weight	1279:1284	arg1	molecules					1286:1294	low molecular weight molecules	1265:1294	low molecular weight molecules from macro and micro-capsules	1265:1324	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	7	9	theme	Scanning	1042:1049	arg1	Calorimetry					1051:1061	Differential Scanning Calorimetry	1029:1061	Differential Scanning Calorimetry	1029:1061	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	6	10	theme	entrapped	877:885	arg1	vitamin					887:893	the entrapped vitamin	873:893	the entrapped vitamin	873:893	In contrast to simple and composite capsules, coated capsules showed a pH-dependent release of the entrapped vitamin especially after shellac crosslinking with calcium.
27083835	7	11	theme	coated	958:963	arg1	capsules					965:972	coated capsules	958:972	coated capsules above the glass transition temperature	958:1011	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	1	12	theme	external	286:293	arg1	capsules					309:316	coated capsules	302:316	coated capsules	302:316	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	1	12	theme	external	286:293	arg1	shellac					202:208	shellac	202:208	shellac	202:208	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	1	12	theme	external	286:293	arg1	layer					295:299	an additional external layer	272:299	an additional external layer (coated capsules)	272:317	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	0	13	theme	riboflavin	109:118	arg1	release					120:126	riboflavin release	109:126	riboflavin release	109:126	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.
27083835	0	14	from	Influence	71:79	arg1	release					120:126	riboflavin release	109:126	riboflavin release	109:126	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.
27083835	2	15	theme	shellac	355:361	arg1	concentration					363:375	shellac concentration	355:375	shellac concentration	355:375	The influence of pH, coating time, shellac concentration and preparation mechanism (acid or calcium precipitation) were investigated.
27083835	5	16	theme	Optical	662:668	arg1	microscopy					706:715	Optical, fluorescence and scanning electron microscopy	662:715	microscopy	706:715	Optical, fluorescence and scanning electron microscopy, highlighted different capsules and membranes architectures.
27083835	5	17	dep	capsules	740:747	arg1	architectures					763:775	architectures	763:775	architectures	763:775	Optical, fluorescence and scanning electron microscopy, highlighted different capsules and membranes architectures.
27083835	5	18	theme	fluorescence	671:682	arg1	microscopy					706:715	Optical, fluorescence and scanning electron microscopy	662:715	microscopy	706:715	Optical, fluorescence and scanning electron microscopy, highlighted different capsules and membranes architectures.
27083835	7	19	theme	Differential	1029:1040	arg1	Calorimetry					1051:1061	Differential Scanning Calorimetry	1029:1061	Differential Scanning Calorimetry	1029:1061	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	6	20	theme	pH-dependent	849:860	arg1	release					862:868	a pH-dependent release	847:868	a pH-dependent release of the entrapped vitamin	847:893	In contrast to simple and composite capsules, coated capsules showed a pH-dependent release of the entrapped vitamin especially after shellac crosslinking with calcium.
27083835	0	21	theme	Physico-chemical	0:15	arg1	properties					17:26	Physico-chemical properties	0:26	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.	0:127	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.
27083835	8	22	theme	molecules	1286:1294	arg1	mechanism					1252:1260	control release mechanism	1236:1260	control release mechanism of low molecular weight molecules from macro and micro-capsules	1236:1324	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	5	23	theme	scanning	688:695	arg1	microscopy					706:715	Optical, fluorescence and scanning electron microscopy	662:715	microscopy	706:715	Optical, fluorescence and scanning electron microscopy, highlighted different capsules and membranes architectures.
27083835	2	24	theme	preparation	381:391	arg1	mechanism					393:401	preparation mechanism	381:401	preparation mechanism	381:401	The influence of pH, coating time, shellac concentration and preparation mechanism (acid or calcium precipitation) were investigated.
27083835	0	25	theme	alginate/shellac	31:46	arg1	capsules					61:68	alginate/shellac aqueous-core capsules	31:68	alginate/shellac aqueous-core capsules	31:68	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.
27083835	1	26	theme	coated	302:307	arg1	capsules					309:316	coated capsules	302:316	coated capsules	302:316	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	1	26	theme	coated	302:307	arg1	layer					295:299	an additional external layer	272:299	an additional external layer (coated capsules)	272:317	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	7	27	theme	acidic	1183:1188	arg1	conditions					1190:1199	acidic conditions	1183:1199	acidic conditions	1183:1199	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	2	28	dep	time	349:352	arg1	acid					404:407	acid	404:407	acid	404:407	The influence of pH, coating time, shellac concentration and preparation mechanism (acid or calcium precipitation) were investigated.
27083835	2	28	dep	time	349:352	arg1	precipitation					420:432	calcium precipitation	412:432	calcium precipitation	412:432	The influence of pH, coating time, shellac concentration and preparation mechanism (acid or calcium precipitation) were investigated.
27083835	4	29	theme	preparation	600:610	arg1	conditions					612:621	the preparation conditions	596:621	the preparation conditions	596:621	The feasibility of shellac incorporation was closely related to the preparation conditions as confirmed by Infrared spectroscopy.
27083835	0	30	theme	capsules	61:68	arg1	properties					17:26	Physico-chemical properties	0:26	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.	0:127	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.
27083835	6	31	with	crosslinking	920:931	arg1	calcium					938:944	calcium	938:944	calcium	938:944	In contrast to simple and composite capsules, coated capsules showed a pH-dependent release of the entrapped vitamin especially after shellac crosslinking with calcium.
27083835	2	32	theme	calcium	412:418	arg1	precipitation					420:432	calcium precipitation	412:432	calcium precipitation	412:432	The influence of pH, coating time, shellac concentration and preparation mechanism (acid or calcium precipitation) were investigated.
27083835	0	33	theme	aqueous-core	48:59	arg1	capsules					61:68	alginate/shellac aqueous-core capsules	31:68	alginate/shellac aqueous-core capsules	31:68	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.
27083835	4	34	theme	incorporation	559:571	arg1	feasibility					536:546	The feasibility	532:546	The feasibility of shellac incorporation	532:571	The feasibility of shellac incorporation was closely related to the preparation conditions as confirmed by Infrared spectroscopy.
27083835	4	34	theme	incorporation	559:571	arg1	related					585:591	related	585:591	related	585:591	The feasibility of shellac incorporation was closely related to the preparation conditions as confirmed by Infrared spectroscopy.
27083835	8	35	theme	global	1207:1212	arg1	approach					1214:1221	This global approach	1202:1221	This global approach	1202:1221	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	8	36	from	mechanism	1252:1260	arg1	micro-capsules					1311:1324	micro-capsules	1311:1324	micro-capsules	1311:1324	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	8	36	from	mechanism	1252:1260	arg1	macro					1301:1305	macro	1301:1305	macro	1301:1305	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	1	37	theme	composite	246:254	arg1	membrane					236:243	the membrane	232:243	the membrane (composite capsules)	232:264	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	1	37	theme	composite	246:254	arg1	capsules					256:263	composite capsules	246:263	composite capsules	246:263	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	4	38	theme	shellac	551:557	arg1	incorporation					559:571	shellac incorporation	551:571	shellac incorporation	551:571	The feasibility of shellac incorporation was closely related to the preparation conditions as confirmed by Infrared spectroscopy.
27083835	5	39	theme	different	730:738	arg1	capsules					740:747	different capsules	730:747	different capsules	730:747	Optical, fluorescence and scanning electron microscopy, highlighted different capsules and membranes architectures.
27083835	6	40	theme	coated	824:829	arg1	capsules					831:838	coated capsules	824:838	coated capsules	824:838	In contrast to simple and composite capsules, coated capsules showed a pH-dependent release of the entrapped vitamin especially after shellac crosslinking with calcium.
27083835	3	41	theme	capsules	511:518	arg1	properties					520:529	the capsules properties	507:529	the capsules properties	507:529	Results showed that shellac significantly influenced the capsules properties.
27083835	8	42	from	macro	1301:1305	arg1	molecules					1286:1294	low molecular weight molecules	1265:1294	low molecular weight molecules from macro and micro-capsules	1265:1324	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	8	42	from	macro	1301:1305	arg1	mechanism					1252:1260	control release mechanism	1236:1260	control release mechanism of low molecular weight molecules from macro and micro-capsules	1236:1324	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	7	43	theme	riboflavin	1156:1165	arg1	retention					1167:1175	riboflavin retention	1156:1175	riboflavin retention	1156:1175	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	1	44	theme	physico-chemical	140:155	arg1	properties					157:166	physico-chemical properties	140:166	physico-chemical properties of alginate liquid-core capsules	140:199	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	7	45	theme	transition	990:999	arg1	temperature					1001:1011	the glass transition temperature	980:1011	the glass transition temperature	980:1011	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	7	46	theme	shellac	1135:1141	arg1	behavior					1123:1130	thermoplastic behavior	1109:1130	thermoplastic behavior of shellac	1109:1141	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	6	47	theme	shellac	912:918	arg1	crosslinking					920:931	shellac crosslinking	912:931	shellac crosslinking with calcium	912:944	In contrast to simple and composite capsules, coated capsules showed a pH-dependent release of the entrapped vitamin especially after shellac crosslinking with calcium.
27083835	8	48	theme	control	1236:1242	arg1	mechanism					1252:1260	control release mechanism	1236:1260	control release mechanism of low molecular weight molecules from macro and micro-capsules	1236:1324	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	7	49	theme	thermoplastic	1109:1121	arg1	behavior					1123:1130	thermoplastic behavior	1109:1130	thermoplastic behavior of shellac	1109:1141	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	7	50	theme	irreversible	1071:1082	arg1	change					1095:1100	irreversible structural change	1071:1100	irreversible structural change due to thermoplastic behavior of shellac	1071:1141	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	6	51	theme	composite	804:812	arg1	capsules					814:821	simple and composite capsules	793:821	simple and composite capsules	793:821	In contrast to simple and composite capsules, coated capsules showed a pH-dependent release of the entrapped vitamin especially after shellac crosslinking with calcium.
27083835	2	52	theme	pH	337:338	arg1	influence					324:332	The influence	320:332	The influence of pH, coating time, shellac concentration and preparation mechanism (acid or calcium precipitation)	320:433	The influence of pH, coating time, shellac concentration and preparation mechanism (acid or calcium precipitation) were investigated.
27083835	4	53	theme	Infrared	639:646	arg1	spectroscopy					648:659	Infrared spectroscopy	639:659	Infrared spectroscopy	639:659	The feasibility of shellac incorporation was closely related to the preparation conditions as confirmed by Infrared spectroscopy.
27083835	0	54	theme	membrane	84:91	arg1	architecture					93:104	membrane architecture	84:104	membrane architecture	84:104	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.
27083835	0	55	dep	properties	17:26	arg1	Influence					71:79	Influence	71:79	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.	0:127	Physico-chemical properties of alginate/shellac aqueous-core capsules: Influence of membrane architecture on riboflavin release.
27083835	1	56	theme	alginate	171:178	arg1	capsules					192:199	alginate liquid-core capsules	171:199	alginate liquid-core capsules	171:199	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	7	57	theme	glass	984:988	arg1	temperature					1001:1011	the glass transition temperature	980:1011	the glass transition temperature	980:1011	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	8	58	theme	release	1244:1250	arg1	mechanism					1252:1260	control release mechanism	1236:1260	control release mechanism of low molecular weight molecules from macro and micro-capsules	1236:1324	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
27083835	6	59	theme	simple	793:798	arg1	capsules					814:821	simple and composite capsules	793:821	simple and composite capsules	793:821	In contrast to simple and composite capsules, coated capsules showed a pH-dependent release of the entrapped vitamin especially after shellac crosslinking with calcium.
27083835	1	60	theme	liquid-core	180:190	arg1	capsules					192:199	alginate liquid-core capsules	171:199	alginate liquid-core capsules	171:199	To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules).
27083835	7	61	theme	capsules	965:972	arg1	Heating					947:953	Heating	947:953	Heating of coated capsules above the glass transition temperature	947:1011	Heating of coated capsules above the glass transition temperature investigated by Differential Scanning Calorimetry, led to irreversible structural change due to thermoplastic behavior of shellac and enhanced riboflavin retention under acidic conditions.
27083835	8	62	theme	molecular	1269:1277	arg1	molecules					1286:1294	low molecular weight molecules	1265:1294	low molecular weight molecules from macro and micro-capsules	1265:1324	This global approach is useful to control release mechanism of low molecular weight molecules from macro and micro-capsules.
24672328	0	0	theme	aceclofenac	84:94	arg1	delivery					72:79	transdermal delivery	60:79	transdermal delivery of aceclofenac	60:94	Formulation and evaluation of guggul lipid nanovesicles for transdermal delivery of aceclofenac.
24672328	6	1	dep	vesicles	710:717	arg1	GL					727:728	GL	727:728	GL	727:728	RESULTS Both categories of vesicles (PC and GL) showed optimum physicochemical properties; however, accelerated stability study showed considerable differences.
24672328	6	1	dep	vesicles	710:717	arg1	PC					720:721	PC	720:721	PC	720:721	RESULTS Both categories of vesicles (PC and GL) showed optimum physicochemical properties; however, accelerated stability study showed considerable differences.
24672328	6	1	dep	vesicles	710:717	arg1	vesicles					710:717	vesicles	710:717	vesicles (PC and GL)	710:729	RESULTS Both categories of vesicles (PC and GL) showed optimum physicochemical properties; however, accelerated stability study showed considerable differences.
24672328	12	2	theme	vesicle	1404:1410	arg1	wall					1412:1415	vesicle wall	1404:1415	vesicle wall with cholesterol	1404:1432	Guggul lipid being a planar molecule might be stacked in vesicle wall with cholesterol.
24672328	11	3	theme	good	1234:1237	arg1	retention					1244:1252	good drug retention	1234:1252	good drug retention	1234:1252	The formulation with higher lipid content (GL-1) showed good drug retention after 24 hours and appreciable stability both at higher temperature and for longer duration.
24672328	1	4	contain	have	120:123	arg1	drugs					114:118	CONTEXT Most new drugs	97:118	CONTEXT Most new drugs	97:118	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	1	4	contain	have	120:123	arg2	solubility					135:144	low water solubility	125:144	low water solubility	125:144	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	11	5	theme	longer	1330:1335	arg1	duration					1337:1344	longer duration	1330:1344	longer duration	1330:1344	The formulation with higher lipid content (GL-1) showed good drug retention after 24 hours and appreciable stability both at higher temperature and for longer duration.
24672328	14	6	theme	stable	1579:1584	arg1	vesicles					1586:1593	stable vesicles	1579:1593	stable vesicles	1579:1593	Guggul lipid is suitable for producing stable vesicles.
24672328	5	7	theme	anti-inflammatory	639:655	arg1	activity					657:664	anti-inflammatory activity	639:664	anti-inflammatory activity	639:664	Then its skin permeation and anti-inflammatory activity were determined.
24672328	13	8	dep	CONCLUSION	1435:1444	arg1	played					1481:1486	played	1481:1486	played an important role in stability and drug permeation	1481:1537	CONCLUSION The composition of the nanovesicle played an important role in stability and drug permeation.
24672328	2	9	theme	OBJECTIVE	283:291	arg1	nanovesicles					305:316	OBJECTIVE Aceclofenac nanovesicles	283:316	OBJECTIVE Aceclofenac nanovesicles	283:316	OBJECTIVE Aceclofenac nanovesicles were made using guggul lipid for formulating stable transdermal formulation.
24672328	10	10	theme	PC	1105:1106	arg1	liposomes					1108:1116	PC liposomes	1105:1116	PC liposomes	1105:1116	PC liposomes are unstable at higher temperature and upon longer storage.
24672328	14	11	theme	Guggul	1540:1545	arg1	lipid					1547:1551	Guggul lipid	1540:1551	Guggul lipid	1540:1551	Guggul lipid is suitable for producing stable vesicles.
24672328	10	12	from	temperature	1141:1151	arg1	unstable					1122:1129	unstable	1122:1129	unstable	1122:1129	PC liposomes are unstable at higher temperature and upon longer storage.
24672328	1	13	theme	such	201:204	arg1	drugs					206:210	such drugs	201:210	such drugs	201:210	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	6	14	theme	physicochemical	746:760	arg1	properties					762:771	optimum physicochemical properties	738:771	optimum physicochemical properties	738:771	RESULTS Both categories of vesicles (PC and GL) showed optimum physicochemical properties; however, accelerated stability study showed considerable differences.
24672328	3	15	theme	Guggul	417:422	arg1	lipid					424:428	MATERIALS AND METHODS Guggul lipid	395:428	MATERIALS AND METHODS Guggul lipid	395:428	MATERIALS AND METHODS Guggul lipid was formulated into vesicles along with cholesterol and dicetyl phosphate using film hydration method.
24672328	8	16	theme	edema	1054:1058	arg1	production					1060:1069	edema production	1054:1069	edema production	1054:1069	GLG-1 inhibited edema production by 90.81% in 6 hours.
24672328	6	17	theme	optimum	738:744	arg1	properties					762:771	optimum physicochemical properties	738:771	optimum physicochemical properties	738:771	RESULTS Both categories of vesicles (PC and GL) showed optimum physicochemical properties; however, accelerated stability study showed considerable differences.
24672328	1	18	theme	important	165:173	arg1	formulation					175:185	an important formulation	162:185	an important formulation to administer such drugs	162:210	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	1	18	theme	important	165:173	arg1	liposome					150:157	liposome	150:157	liposome	150:157	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	3	19	theme	MATERIALS	395:403	arg1	lipid					424:428	MATERIALS AND METHODS Guggul lipid	395:428	MATERIALS AND METHODS Guggul lipid	395:428	MATERIALS AND METHODS Guggul lipid was formulated into vesicles along with cholesterol and dicetyl phosphate using film hydration method.
24672328	11	20	theme	appreciable	1273:1283	arg1	stability					1285:1293	appreciable stability	1273:1293	appreciable stability	1273:1293	The formulation with higher lipid content (GL-1) showed good drug retention after 24 hours and appreciable stability both at higher temperature and for longer duration.
24672328	11	21	theme	higher	1199:1204	arg1	GL-1					1221:1224	GL-1	1221:1224	GL-1	1221:1224	The formulation with higher lipid content (GL-1) showed good drug retention after 24 hours and appreciable stability both at higher temperature and for longer duration.
24672328	11	21	theme	higher	1199:1204	arg1	content					1212:1218	higher lipid content	1199:1218	higher lipid content (GL-1)	1199:1225	The formulation with higher lipid content (GL-1) showed good drug retention after 24 hours and appreciable stability both at higher temperature and for longer duration.
24672328	7	22	theme	Corresponding	899:911	arg1	GLG-1					929:933	GLG-1	929:933	GLG-1	929:933	GL-1 was appreciably stable for over 6 months at 4 °C. Corresponding gels (PCG-1 and GLG-1) showed C max values at 4.98 and 7.32 μg/mL along with the Tmax values at 4 and 8 hours, respectively.
24672328	7	22	theme	Corresponding	899:911	arg1	PCG-1					919:923	PCG-1	919:923	PCG-1	919:923	GL-1 was appreciably stable for over 6 months at 4 °C. Corresponding gels (PCG-1 and GLG-1) showed C max values at 4.98 and 7.32 μg/mL along with the Tmax values at 4 and 8 hours, respectively.
24672328	7	22	theme	Corresponding	899:911	arg1	gels					913:916	Corresponding gels	899:916	Corresponding gels (PCG-1 and GLG-1)	899:934	GL-1 was appreciably stable for over 6 months at 4 °C. Corresponding gels (PCG-1 and GLG-1) showed C max values at 4.98 and 7.32 μg/mL along with the Tmax values at 4 and 8 hours, respectively.
24672328	13	23	theme	important	1491:1499	arg1	role					1501:1504	an important role	1488:1504	an important role	1488:1504	CONCLUSION The composition of the nanovesicle played an important role in stability and drug permeation.
24672328	1	24	theme	negligible	251:260	arg1	absorption					271:280	negligible systemic absorption	251:280	negligible systemic absorption	251:280	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	11	25	with	formulation	1182:1192	arg1	GL-1					1221:1224	GL-1	1221:1224	GL-1	1221:1224	The formulation with higher lipid content (GL-1) showed good drug retention after 24 hours and appreciable stability both at higher temperature and for longer duration.
24672328	11	25	with	formulation	1182:1192	arg1	content					1212:1218	higher lipid content	1199:1218	higher lipid content (GL-1)	1199:1225	The formulation with higher lipid content (GL-1) showed good drug retention after 24 hours and appreciable stability both at higher temperature and for longer duration.
24672328	5	26	theme	skin	619:622	arg1	permeation					624:633	its skin permeation	615:633	its skin permeation	615:633	Then its skin permeation and anti-inflammatory activity were determined.
24672328	1	27	theme	CONTEXT	97:103	arg1	drugs					114:118	CONTEXT Most new drugs	97:118	CONTEXT Most new drugs	97:118	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	11	28	theme	higher	1303:1308	arg1	temperature					1310:1320	higher temperature	1303:1320	higher temperature	1303:1320	The formulation with higher lipid content (GL-1) showed good drug retention after 24 hours and appreciable stability both at higher temperature and for longer duration.
24672328	1	29	theme	systemic	262:269	arg1	absorption					271:280	negligible systemic absorption	251:280	negligible systemic absorption	251:280	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	12	30	with	wall	1412:1415	arg1	cholesterol					1422:1432	cholesterol	1422:1432	cholesterol	1422:1432	Guggul lipid being a planar molecule might be stacked in vesicle wall with cholesterol.
24672328	2	31	theme	Aceclofenac	293:303	arg1	nanovesicles					305:316	OBJECTIVE Aceclofenac nanovesicles	283:316	OBJECTIVE Aceclofenac nanovesicles	283:316	OBJECTIVE Aceclofenac nanovesicles were made using guggul lipid for formulating stable transdermal formulation.
24672328	11	32	theme	lipid	1206:1210	arg1	GL-1					1221:1224	GL-1	1221:1224	GL-1	1221:1224	The formulation with higher lipid content (GL-1) showed good drug retention after 24 hours and appreciable stability both at higher temperature and for longer duration.
24672328	11	32	theme	lipid	1206:1210	arg1	content					1212:1218	higher lipid content	1199:1218	higher lipid content (GL-1)	1199:1225	The formulation with higher lipid content (GL-1) showed good drug retention after 24 hours and appreciable stability both at higher temperature and for longer duration.
24672328	1	33	theme	new	110:112	arg1	drugs					114:118	CONTEXT Most new drugs	97:118	CONTEXT Most new drugs	97:118	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	3	34	theme	dicetyl	486:492	arg1	phosphate					494:502	dicetyl phosphate	486:502	dicetyl phosphate	486:502	MATERIALS AND METHODS Guggul lipid was formulated into vesicles along with cholesterol and dicetyl phosphate using film hydration method.
24672328	0	35	theme	lipid	37:41	arg1	nanovesicles					43:54	guggul lipid nanovesicles	30:54	guggul lipid nanovesicles	30:54	Formulation and evaluation of guggul lipid nanovesicles for transdermal delivery of aceclofenac.
24672328	3	36	theme	hydration	515:523	arg1	method					525:530	film hydration method	510:530	film hydration method	510:530	MATERIALS AND METHODS Guggul lipid was formulated into vesicles along with cholesterol and dicetyl phosphate using film hydration method.
24672328	7	37	theme	C	943:943	arg1	values					949:954	C max values	943:954	C max values	943:954	GL-1 was appreciably stable for over 6 months at 4 °C. Corresponding gels (PCG-1 and GLG-1) showed C max values at 4.98 and 7.32 μg/mL along with the Tmax values at 4 and 8 hours, respectively.
24672328	7	38	from	hours	1017:1021	arg1	values					999:1004	the Tmax values	990:1004	the Tmax values	990:1004	GL-1 was appreciably stable for over 6 months at 4 °C. Corresponding gels (PCG-1 and GLG-1) showed C max values at 4.98 and 7.32 μg/mL along with the Tmax values at 4 and 8 hours, respectively.
24672328	0	39	theme	guggul	30:35	arg1	nanovesicles					43:54	guggul lipid nanovesicles	30:54	guggul lipid nanovesicles	30:54	Formulation and evaluation of guggul lipid nanovesicles for transdermal delivery of aceclofenac.
24672328	12	40	theme	planar	1368:1373	arg1	molecule					1375:1382	a planar molecule	1366:1382	a planar molecule	1366:1382	Guggul lipid being a planar molecule might be stacked in vesicle wall with cholesterol.
24672328	6	41	theme	considerable	818:829	arg1	differences					831:841	considerable differences	818:841	considerable differences	818:841	RESULTS Both categories of vesicles (PC and GL) showed optimum physicochemical properties; however, accelerated stability study showed considerable differences.
24672328	13	42	theme	nanovesicle	1469:1479	arg1	composition					1450:1460	The composition	1446:1460	The composition of the nanovesicle	1446:1479	CONCLUSION The composition of the nanovesicle played an important role in stability and drug permeation.
24672328	3	43	theme	METHODS	409:415	arg1	lipid					424:428	MATERIALS AND METHODS Guggul lipid	395:428	MATERIALS AND METHODS Guggul lipid	395:428	MATERIALS AND METHODS Guggul lipid was formulated into vesicles along with cholesterol and dicetyl phosphate using film hydration method.
24672328	11	44	theme	drug	1239:1242	arg1	retention					1244:1252	good drug retention	1234:1252	good drug retention	1234:1252	The formulation with higher lipid content (GL-1) showed good drug retention after 24 hours and appreciable stability both at higher temperature and for longer duration.
24672328	12	45	theme	Guggul	1347:1352	arg1	lipid					1354:1358	Guggul lipid	1347:1358	Guggul lipid being a planar molecule	1347:1382	Guggul lipid being a planar molecule might be stacked in vesicle wall with cholesterol.
24672328	3	46	theme	film	510:513	arg1	method					525:530	film hydration method	510:530	film hydration method	510:530	MATERIALS AND METHODS Guggul lipid was formulated into vesicles along with cholesterol and dicetyl phosphate using film hydration method.
24672328	0	47	theme	nanovesicles	43:54	arg1	evaluation					16:25	evaluation	16:25	evaluation	16:25	Formulation and evaluation of guggul lipid nanovesicles for transdermal delivery of aceclofenac.
24672328	0	47	theme	nanovesicles	43:54	arg1	Formulation					0:10	Formulation	0:10	Formulation	0:10	Formulation and evaluation of guggul lipid nanovesicles for transdermal delivery of aceclofenac.
24672328	4	48	theme	physicochemical	568:582	arg1	properties					584:593	physicochemical properties	568:593	physicochemical properties	568:593	The formulations were analyzed for physicochemical properties and stability.
24672328	7	49	dep	gels	913:916	arg1	GLG-1					929:933	GLG-1	929:933	GLG-1	929:933	GL-1 was appreciably stable for over 6 months at 4 °C. Corresponding gels (PCG-1 and GLG-1) showed C max values at 4.98 and 7.32 μg/mL along with the Tmax values at 4 and 8 hours, respectively.
24672328	7	49	dep	gels	913:916	arg1	PCG-1					919:923	PCG-1	919:923	PCG-1	919:923	GL-1 was appreciably stable for over 6 months at 4 °C. Corresponding gels (PCG-1 and GLG-1) showed C max values at 4.98 and 7.32 μg/mL along with the Tmax values at 4 and 8 hours, respectively.
24672328	7	49	dep	gels	913:916	arg1	gels					913:916	Corresponding gels	899:916	Corresponding gels (PCG-1 and GLG-1)	899:934	GL-1 was appreciably stable for over 6 months at 4 °C. Corresponding gels (PCG-1 and GLG-1) showed C max values at 4.98 and 7.32 μg/mL along with the Tmax values at 4 and 8 hours, respectively.
24672328	2	50	theme	guggul	334:339	arg1	lipid					341:345	guggul lipid	334:345	guggul lipid for formulating stable transdermal formulation	334:392	OBJECTIVE Aceclofenac nanovesicles were made using guggul lipid for formulating stable transdermal formulation.
24672328	7	51	theme	max	945:947	arg1	values					949:954	C max values	943:954	C max values	943:954	GL-1 was appreciably stable for over 6 months at 4 °C. Corresponding gels (PCG-1 and GLG-1) showed C max values at 4.98 and 7.32 μg/mL along with the Tmax values at 4 and 8 hours, respectively.
24672328	10	52	theme	higher	1134:1139	arg1	temperature					1141:1151	higher temperature	1134:1151	higher temperature	1134:1151	PC liposomes are unstable at higher temperature and upon longer storage.
24672328	7	53	from	°C.	895:897	arg1	stable					865:870	stable	865:870	stable	865:870	GL-1 was appreciably stable for over 6 months at 4 °C. Corresponding gels (PCG-1 and GLG-1) showed C max values at 4.98 and 7.32 μg/mL along with the Tmax values at 4 and 8 hours, respectively.
24672328	1	54	contain	has	247:249	arg1	it					222:223	it	222:223	it	222:223	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	1	54	contain	has	247:249	arg2	absorption					271:280	negligible systemic absorption	251:280	negligible systemic absorption	251:280	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	6	55	theme	stability	795:803	arg1	study					805:809	accelerated stability study	783:809	accelerated stability study	783:809	RESULTS Both categories of vesicles (PC and GL) showed optimum physicochemical properties; however, accelerated stability study showed considerable differences.
24672328	1	56	theme	low	125:127	arg1	solubility					135:144	low water solubility	125:144	low water solubility	125:144	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	1	57	theme	Most	105:108	arg1	drugs					114:118	CONTEXT Most new drugs	97:118	CONTEXT Most new drugs	97:118	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	6	58	dep	RESULTS	683:689	arg1	Both					691:694	Both	691:694	Both	691:694	RESULTS Both categories of vesicles (PC and GL) showed optimum physicochemical properties; however, accelerated stability study showed considerable differences.
24672328	6	59	theme	accelerated	783:793	arg1	study					805:809	accelerated stability study	783:809	accelerated stability study	783:809	RESULTS Both categories of vesicles (PC and GL) showed optimum physicochemical properties; however, accelerated stability study showed considerable differences.
24672328	1	60	theme	water	129:133	arg1	solubility					135:144	low water solubility	125:144	low water solubility	125:144	CONTEXT Most new drugs have low water solubility and liposome is an important formulation to administer such drugs; however, it is quite unstable and has negligible systemic absorption.
24672328	2	61	theme	transdermal	370:380	arg1	formulation					382:392	stable transdermal formulation	363:392	stable transdermal formulation	363:392	OBJECTIVE Aceclofenac nanovesicles were made using guggul lipid for formulating stable transdermal formulation.
24672328	6	62	theme	vesicles	710:717	arg1	RESULTS					683:689	RESULTS	683:689	RESULTS	683:689	RESULTS Both categories of vesicles (PC and GL) showed optimum physicochemical properties; however, accelerated stability study showed considerable differences.
24672328	0	63	theme	transdermal	60:70	arg1	delivery					72:79	transdermal delivery	60:79	transdermal delivery of aceclofenac	60:94	Formulation and evaluation of guggul lipid nanovesicles for transdermal delivery of aceclofenac.
24672328	13	64	theme	drug	1523:1526	arg1	permeation					1528:1537	drug permeation	1523:1537	drug permeation	1523:1537	CONCLUSION The composition of the nanovesicle played an important role in stability and drug permeation.
24672328	7	65	theme	Tmax	994:997	arg1	values					999:1004	the Tmax values	990:1004	the Tmax values	990:1004	GL-1 was appreciably stable for over 6 months at 4 °C. Corresponding gels (PCG-1 and GLG-1) showed C max values at 4.98 and 7.32 μg/mL along with the Tmax values at 4 and 8 hours, respectively.
24672328	2	66	theme	stable	363:368	arg1	formulation					382:392	stable transdermal formulation	363:392	stable transdermal formulation	363:392	OBJECTIVE Aceclofenac nanovesicles were made using guggul lipid for formulating stable transdermal formulation.
24672328	10	67	theme	longer	1162:1167	arg1	storage					1169:1175	longer storage	1162:1175	longer storage	1162:1175	PC liposomes are unstable at higher temperature and upon longer storage.
28571991	4	0	theme	matter	692:697	arg1	amounts					642:648	different amounts	632:648	different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat)	632:737	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	0	theme	matter	692:697	arg1	matter					692:697	dry matter	688:697	dry matter (DM)	688:702	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	0	theme	matter	692:697	arg1	MR					653:654	MR	653:654	MR ranging from 0.44 to 1.10 kg	653:683	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	0	theme	matter	692:697	arg1	DM					700:701	DM	700:701	DM	700:701	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	10	1	theme	intake	1545:1550	arg1	regression					1438:1447	Multiple and linear regression	1418:1447	regression	1438:1447	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	3	2	theme	organic	427:433	arg1	matter					435:440	organic matter	427:440	organic matter	427:440	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	10	3	theme	SAS	1646:1648	arg1	REG					1639:1641	PROC REG	1634:1641	PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC)	1634:1692	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	15	4	from	relationship	2408:2419	arg1	contrast					2424:2431	contrast	2424:2431	contrast to the previously observed result in calves still consuming milk replacer	2424:2505	Postweaning, TTSD decreased linearly as FS increased (coefficient of determination = 0.86), whereas FS and SI were unrelated, a relationship in contrast to the previously observed result in calves still consuming milk replacer.
28571991	6	5	theme	=	939:939	arg1	n					937:937	n = 20	937:942	n = 20	937:942	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	6	5	theme	=	939:939	arg1	3					934:934	3	934:934	3	934:934	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	14	6	theme	variation	2145:2153	arg1	±0.33					2255:2259	±0.33	2255:2259	±0.33	2255:2259	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	14	6	theme	variation	2145:2153	arg1	%					2136:2136	92%	2134:2136	92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)]	2134:2237	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	14	6	theme	variation	2145:2153	arg1	variation					2145:2153	the variation	2141:2153	the variation	2141:2153	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	1	7	theme	dairy	217:221	arg1	cows					223:226	dairy cows	217:226	dairy cows	217:226	Fecal starch (FS) has been used as a tool to evaluate starch and diet digestibility in lactating dairy cows and feedlot steers.
28571991	12	8	theme	age	1878:1880	arg1	wk					1872:1873	3 wk	1870:1873	3 wk of age	1870:1880	At 3 wk of age, TTSD increased (coefficient of determination = 0.53) and SI decreased (coefficient of determination = 0.20) with increasing FS.
28571991	9	9	theme	collection	1398:1407	arg1	periods					1409:1415	collection periods	1398:1415	collection periods	1398:1415	Milk replacer and starter intakes (offered and refused) were recorded daily during collection periods.
28571991	3	10	theme	milk	457:460	arg1	rate					484:487	milk replacer (MR) feeding rate	457:487	milk replacer (MR) feeding rate	457:487	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	14	11	from	%	2136:2136	arg1	TTSD					2158:2161	TTSD	2158:2161	TTSD	2158:2161	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	10	12	dep	version	1651:1657	arg1	NC					1690:1691	NC	1690:1691	NC	1690:1691	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	12	dep	version	1651:1657	arg1	Cary					1684:1687	Cary	1684:1687	Cary	1684:1687	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	13	theme	digestibility	1467:1479	arg1	regression					1438:1447	Multiple and linear regression	1418:1447	regression	1438:1447	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	2	14	theme	on-farm	253:259	arg1	advisors					261:268	Some on-farm advisors	248:268	Some on-farm advisors	248:268	Some on-farm advisors also use FS to evaluate calf starter digestibility in preweaned dairy calves.
28571991	4	15	theme	crude	715:719	arg1	protein					721:727	crude protein	715:727	27% crude protein	711:727	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	10	16	theme	organic	1452:1458	arg1	digestibility					1467:1479	organic matter digestibility	1452:1479	organic matter digestibility (% of DM)	1452:1489	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	0	17	theme	preweaned	96:104	arg1	calves					112:117	preweaned dairy calves	96:117	preweaned dairy calves	96:117	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	8	18	theme	acid-insoluble	1256:1269	arg1	ash					1271:1273	acid-insoluble ash	1256:1273	acid-insoluble ash	1256:1273	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	8	18	theme	acid-insoluble	1256:1269	arg1	marker					1299:1304	an internal marker	1287:1304	an internal marker at 8 wk	1287:1312	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	0	19	from	indicator	61:69	arg1	calves					112:117	preweaned dairy calves	96:117	preweaned dairy calves	96:117	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	4	20	theme	Male	546:549	arg1	calves					560:565	Male Holstein calves	546:565	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm	546:621	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	10	21	theme	DM	1487:1488	arg1	%					1482:1482	%	1482:1482	% of DM	1482:1488	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	21	theme	DM	1487:1488	arg1	DM					1487:1488	DM	1487:1488	DM	1487:1488	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	14	22	theme	×	2225:2225	arg1	FS					2227:2228	[0.6 × FS (±0.04)	2220:2236	[0.6 × FS (±0.04)	2220:2236	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	16	23	dep	good	2543:2546	arg1	estimate					2548:2555	estimate	2548:2555	estimate	2548:2555	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	1	24	dep	starch	174:179	arg1	digestibility					190:202	digestibility	190:202	digestibility	190:202	Fecal starch (FS) has been used as a tool to evaluate starch and diet digestibility in lactating dairy cows and feedlot steers.
28571991	4	25	theme	weight	588:593	arg1	kg					577:578	43 ± 2.9 kg	568:578	43 ± 2.9 kg of body weight	568:593	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	0	26	from	digestion	83:91	arg1	calves					112:117	preweaned dairy calves	96:117	preweaned dairy calves	96:117	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	6	27	theme	Fecal	901:905	arg1	samples					912:918	Fecal grab samples	901:918	Fecal grab samples	901:918	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	15	28	dep	=	2363:2363	arg1	0.86					2365:2368	0.86	2365:2368	0.86	2365:2368	Postweaning, TTSD decreased linearly as FS increased (coefficient of determination = 0.86), whereas FS and SI were unrelated, a relationship in contrast to the previously observed result in calves still consuming milk replacer.
28571991	8	29	from	wk	1311:1312	arg1	ash					1271:1273	acid-insoluble ash	1256:1273	acid-insoluble ash	1256:1273	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	8	29	from	wk	1311:1312	arg1	marker					1299:1304	an internal marker	1287:1304	an internal marker at 8 wk	1287:1312	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	10	30	theme	starch	1504:1509	arg1	TTSD					1526:1529	TTSD	1526:1529	TTSD	1526:1529	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	30	theme	starch	1504:1509	arg1	digestibility					1511:1523	total-tract starch digestibility	1492:1523	total-tract starch digestibility (TTSD; % of DM)	1492:1539	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	6	31	theme	6	946:946	arg1	n					968:968	n = 35	968:973	n = 35	968:973	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	6	31	theme	6	946:946	arg1	wk					964:965	3 (n = 20), 6 (n = 20), and 8 wk	934:965	3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age	934:981	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	5	32	theme	whole	805:809	arg1	corn					811:814	whole corn	805:814	37% whole corn	801:814	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	3	33	theme	preweaned	522:530	arg1	calves					538:543	preweaned dairy calves	522:543	preweaned dairy calves	522:543	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	8	34	theme	internal	1290:1297	arg1	ash					1271:1273	acid-insoluble ash	1256:1273	acid-insoluble ash	1256:1273	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	8	34	theme	internal	1290:1297	arg1	marker					1299:1304	an internal marker	1287:1304	an internal marker at 8 wk	1287:1312	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	4	35	dep	=	598:598	arg1	kg					577:578	43 ± 2.9 kg	568:578	43 ± 2.9 kg of body weight	568:593	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	11	36	dep	×	1793:1793	arg1	age					1816:1818	× age	1814:1818	× age	1814:1818	Prior to weaning, SI, age, and MR rate explained 89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39).
28571991	11	36	dep	×	1793:1793	arg1	MR					1839:1840	[24.8 × MR (±3.19)	1831:1848	[24.8 × MR (±3.19)	1831:1848	Prior to weaning, SI, age, and MR rate explained 89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39).
28571991	10	37	theme	FS	1593:1594	arg1	regression					1438:1447	Multiple and linear regression	1418:1447	regression	1438:1447	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	5	38	theme	protein	837:843	arg1	pellet					845:850	protein pellet	837:850	35% protein pellet	833:850	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	1	39	theme	feedlot	232:238	arg1	steers					240:245	feedlot steers	232:245	feedlot steers	232:245	Fecal starch (FS) has been used as a tool to evaluate starch and diet digestibility in lactating dairy cows and feedlot steers.
28571991	6	40	theme	=	970:970	arg1	n					968:968	n = 35	968:973	n = 35	968:973	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	6	40	theme	=	970:970	arg1	wk					964:965	3 (n = 20), 6 (n = 20), and 8 wk	934:965	3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age	934:981	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	15	41	theme	milk	2493:2496	arg1	replacer					2498:2505	milk replacer	2493:2505	milk replacer	2493:2505	Postweaning, TTSD decreased linearly as FS increased (coefficient of determination = 0.86), whereas FS and SI were unrelated, a relationship in contrast to the previously observed result in calves still consuming milk replacer.
28571991	14	42	theme	×	2204:2204	arg1	MR					2206:2207	[2.4 × MR (±0.56)	2199:2215	[2.4 × MR (±0.56)	2199:2215	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	16	43	from	study	2523:2527	arg1	good					2543:2546	good	2543:2546	good	2543:2546	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	16	43	from	study	2523:2527	arg1	FS					2530:2531	FS	2530:2531	FS	2530:2531	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	11	44	theme	variation	1755:1763	arg1	%					1746:1746	89%	1744:1746	89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39)	1744:1864	Prior to weaning, SI, age, and MR rate explained 89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39).
28571991	11	44	theme	variation	1755:1763	arg1	variation					1755:1763	the variation	1751:1763	the variation	1751:1763	Prior to weaning, SI, age, and MR rate explained 89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39).
28571991	10	45	theme	fecal	1602:1606	arg1	DM					1608:1609	fecal DM	1602:1609	fecal DM	1602:1609	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	4	46	theme	single	611:616	arg1	farm					618:621	a single farm	609:621	a single farm	609:621	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	0	47	theme	dry	74:76	arg1	digestion					83:91	dry feed digestion	74:91	dry feed digestion in preweaned dairy calves	74:117	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	16	48	theme	intake	2577:2582	arg1	good					2543:2546	good	2543:2546	good	2543:2546	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	16	48	theme	intake	2577:2582	arg1	FS					2530:2531	FS	2530:2531	FS	2530:2531	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	15	49	theme	determination	2349:2361	arg1	coefficient					2334:2344	coefficient	2334:2344	coefficient of determination = 0.86	2334:2368	Postweaning, TTSD decreased linearly as FS increased (coefficient of determination = 0.86), whereas FS and SI were unrelated, a relationship in contrast to the previously observed result in calves still consuming milk replacer.
28571991	7	50	theme	equal	1104:1108	arg1	basis					1121:1125	an equal wet-weight basis	1101:1125	an equal wet-weight basis	1101:1125	Twelve fecal samples per calf were taken via rectal palpation over a 5-d period each week, frozen daily, combined on an equal wet-weight basis, and subsampled for analysis.
28571991	3	51	from	concentrations	504:517	arg1	calves					538:543	preweaned dairy calves	522:543	preweaned dairy calves	522:543	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	0	52	theme	digestion	83:91	arg1	indicator					61:69	an indicator	58:69	an indicator of dry feed digestion in preweaned dairy calves	58:117	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	2	53	theme	preweaned	324:332	arg1	calves					340:345	preweaned dairy calves	324:345	preweaned dairy calves	324:345	Some on-farm advisors also use FS to evaluate calf starter digestibility in preweaned dairy calves.
28571991	0	54	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.	0:118	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	15	55	theme	observed	2451:2458	arg1	result					2460:2465	the previously observed result	2436:2465	the previously observed result in calves still consuming milk replacer	2436:2505	Postweaning, TTSD decreased linearly as FS increased (coefficient of determination = 0.86), whereas FS and SI were unrelated, a relationship in contrast to the previously observed result in calves still consuming milk replacer.
28571991	3	56	theme	intake	403:408	arg1	influence					382:390	the influence	378:390	the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves	378:543	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	5	57	theme	±	886:886	arg1	%					891:891	43 ± 1.9%	883:891	43 ± 1.9% starch	883:898	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	4	58	dep	%	713:713	arg1	protein					721:727	crude protein	715:727	27% crude protein	711:727	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	11	59	theme	×	1837:1837	arg1	MR					1839:1840	[24.8 × MR (±3.19)	1831:1848	[24.8 × MR (±3.19)	1831:1848	Prior to weaning, SI, age, and MR rate explained 89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39).
28571991	5	60	theme	ingredient	774:783	arg1	composition					785:795	Starter ingredient composition	766:795	Starter ingredient composition	766:795	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	9	61	theme	starter	1333:1339	arg1	intakes					1341:1347	Milk replacer and starter intakes	1315:1347	intakes	1341:1347	Milk replacer and starter intakes (offered and refused) were recorded daily during collection periods.
28571991	5	62	dep	%	835:835	arg1	pellet					845:850	protein pellet	837:850	35% protein pellet	833:850	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	10	63	dep	TTSD	1526:1529	arg1	DM					1537:1538	DM	1537:1538	DM	1537:1538	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	63	dep	TTSD	1526:1529	arg1	%					1532:1532	%	1532:1532	% of DM	1532:1538	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	6	64	theme	age	979:981	arg1	n					968:968	n = 35	968:973	n = 35	968:973	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	6	64	theme	age	979:981	arg1	wk					964:965	3 (n = 20), 6 (n = 20), and 8 wk	934:965	3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age	934:981	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	12	65	theme	determination	1969:1981	arg1	coefficient					1954:1964	coefficient	1954:1964	coefficient of determination	1954:1981	At 3 wk of age, TTSD increased (coefficient of determination = 0.53) and SI decreased (coefficient of determination = 0.20) with increasing FS.
28571991	4	66	theme	different	632:640	arg1	amounts					642:648	different amounts	632:648	different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat)	632:737	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	66	theme	different	632:640	arg1	matter					692:697	dry matter	688:697	dry matter (DM)	688:702	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	66	theme	different	632:640	arg1	MR					653:654	MR	653:654	MR ranging from 0.44 to 1.10 kg	653:683	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	66	theme	different	632:640	arg1	DM					700:701	DM	700:701	DM	700:701	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	8	67	theme	external	1186:1193	arg1	marker					1209:1214	an external digestibility marker	1183:1214	an external digestibility marker	1183:1214	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	8	67	theme	external	1186:1193	arg1	oxide					1165:1169	Chromic oxide	1157:1169	Chromic oxide	1157:1169	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	16	68	theme	TTSD	2560:2563	arg1	good					2543:2546	good	2543:2546	good	2543:2546	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	16	68	theme	TTSD	2560:2563	arg1	FS					2530:2531	FS	2530:2531	FS	2530:2531	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	0	69	from	calves	112:117	arg1	indicator					61:69	an indicator	58:69	an indicator of dry feed digestion in preweaned dairy calves	58:117	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	3	70	theme	starter	395:401	arg1	SI					411:412	SI	411:412	SI	411:412	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	3	70	theme	starter	395:401	arg1	intake					403:408	starter intake	395:408	starter intake (SI)	395:413	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	3	71	theme	matter	435:440	arg1	digestibility					442:454	starch and organic matter digestibility	416:454	starch and organic matter digestibility	416:454	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	13	72	from	wk	2016:2017	arg1	unrelated					2044:2052	unrelated	2044:2052	unrelated	2044:2052	At 6 wk of age, TTSD and SI were unrelated to FS.
28571991	7	73	theme	5-d	1053:1055	arg1	period					1057:1062	a 5-d period each week	1051:1072	a 5-d period each week	1051:1072	Twelve fecal samples per calf were taken via rectal palpation over a 5-d period each week, frozen daily, combined on an equal wet-weight basis, and subsampled for analysis.
28571991	8	74	theme	Chromic	1157:1163	arg1	marker					1209:1214	an external digestibility marker	1183:1214	an external digestibility marker	1183:1214	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	8	74	theme	Chromic	1157:1163	arg1	oxide					1165:1169	Chromic oxide	1157:1169	Chromic oxide	1157:1169	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	3	75	from	influence	382:390	arg1	concentrations					504:517	FS concentrations	501:517	FS concentrations in preweaned dairy calves	501:543	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	2	76	theme	calf	294:297	arg1	digestibility					307:319	calf starter digestibility	294:319	calf starter digestibility in preweaned dairy calves	294:345	Some on-farm advisors also use FS to evaluate calf starter digestibility in preweaned dairy calves.
28571991	8	77	theme	3	1219:1219	arg1	wk					1227:1228	3 and 6 wk	1219:1228	wk	1227:1228	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	5	78	theme	%	891:891	arg1	starch					893:898	43 ± 1.9% starch	883:898	43 ± 1.9% starch	883:898	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	13	79	theme	age	2022:2024	arg1	wk					2016:2017	6 wk	2014:2017	6 wk of age	2014:2024	At 6 wk of age, TTSD and SI were unrelated to FS.
28571991	4	80	theme	dry	688:690	arg1	matter					692:697	dry matter	688:697	dry matter (DM)	688:702	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	80	theme	dry	688:690	arg1	DM					700:701	DM	700:701	DM	700:701	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	10	81	theme	MR	1542:1543	arg1	kg/d					1553:1556	kg/d	1553:1556	kg/d	1553:1556	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	81	theme	MR	1542:1543	arg1	week					1580:1583	week	1580:1583	week	1580:1583	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	81	theme	MR	1542:1543	arg1	intake					1545:1550	MR intake	1542:1550	MR intake (kg/d)	1542:1557	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	3	82	theme	replacer	462:469	arg1	rate					484:487	milk replacer (MR) feeding rate	457:487	milk replacer (MR) feeding rate	457:487	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	8	83	theme	6	1225:1225	arg1	wk					1227:1228	3 and 6 wk	1219:1228	wk	1227:1228	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	7	84	theme	rectal	1029:1034	arg1	palpation					1036:1044	rectal palpation	1029:1044	rectal palpation	1029:1044	Twelve fecal samples per calf were taken via rectal palpation over a 5-d period each week, frozen daily, combined on an equal wet-weight basis, and subsampled for analysis.
28571991	10	85	theme	PROC	1634:1637	arg1	REG					1639:1641	PROC REG	1634:1641	PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC)	1634:1692	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	5	86	dep	%	803:803	arg1	molasses					860:867	molasses	860:867	molasses	860:867	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	5	86	dep	%	803:803	arg1	corn					811:814	whole corn	805:814	37% whole corn	801:814	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	10	87	theme	linear	1431:1436	arg1	regression					1438:1447	Multiple and linear regression	1418:1447	regression	1438:1447	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	0	88	theme	starch	34:39	arg1	concentration					41:53	fecal starch concentration	28:53	fecal starch concentration	28:53	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	4	89	theme	Holstein	551:558	arg1	calves					560:565	Male Holstein calves	546:565	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm	546:621	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	8	90	used	used	1175:1178	arg2	oxide					1165:1169	Chromic oxide	1157:1169	Chromic oxide	1157:1169	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	8	90	used	used	1175:1178	arg2	marker					1209:1214	an external digestibility marker	1183:1214	an external digestibility marker	1183:1214	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	6	91	theme	3	934:934	arg1	n					968:968	n = 35	968:973	n = 35	968:973	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	6	91	theme	3	934:934	arg1	wk					964:965	3 (n = 20), 6 (n = 20), and 8 wk	934:965	3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age	934:981	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	3	92	theme	feeding	476:482	arg1	rate					484:487	milk replacer (MR) feeding rate	457:487	milk replacer (MR) feeding rate	457:487	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	14	93	from	variation	2145:2153	arg1	TTSD					2158:2161	TTSD	2158:2161	TTSD	2158:2161	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	2	94	from	digestibility	307:319	arg1	calves					340:345	preweaned dairy calves	324:345	preweaned dairy calves	324:345	Some on-farm advisors also use FS to evaluate calf starter digestibility in preweaned dairy calves.
28571991	7	95	theme	fecal	991:995	arg1	samples					997:1003	Twelve fecal samples	984:1003	Twelve fecal samples per calf	984:1012	Twelve fecal samples per calf were taken via rectal palpation over a 5-d period each week, frozen daily, combined on an equal wet-weight basis, and subsampled for analysis.
28571991	16	96	theme	dry	2568:2570	arg1	intake					2577:2582	dry feed intake	2568:2582	dry feed intake	2568:2582	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	10	97	theme	matter	1460:1465	arg1	digestibility					1467:1479	organic matter digestibility	1452:1479	organic matter digestibility (% of DM)	1452:1489	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	0	98	theme	dairy	106:110	arg1	calves					112:117	preweaned dairy calves	96:117	preweaned dairy calves	96:117	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	16	99	contain	has	2611:2613	arg1	good					2543:2546	good	2543:2546	good	2543:2546	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	16	99	contain	has	2611:2613	arg2	potential					2615:2623	potential	2615:2623	potential for evaluating TTSD in calves after weaning	2615:2667	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	16	99	contain	has	2611:2613	arg1	FS					2530:2531	FS	2530:2531	FS	2530:2531	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	3	100	theme	MR	472:473	arg1	rate					484:487	milk replacer (MR) feeding rate	457:487	milk replacer (MR) feeding rate	457:487	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	3	101	theme	rate	484:487	arg1	influence					382:390	the influence	378:390	the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves	378:543	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	11	102	from	%	1746:1746	arg1	TTSD					1768:1771	TTSD	1768:1771	TTSD	1768:1771	Prior to weaning, SI, age, and MR rate explained 89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39).
28571991	14	103	from	TTSD	2158:2161	arg1	±0.33					2255:2259	±0.33	2255:2259	±0.33	2255:2259	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	14	103	from	TTSD	2158:2161	arg1	%					2136:2136	92%	2134:2136	92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)]	2134:2237	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	14	103	from	TTSD	2158:2161	arg1	variation					2145:2153	the variation	2141:2153	the variation	2141:2153	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	10	104	theme	Multiple	1418:1425	arg1	regression					1438:1447	Multiple and linear regression	1418:1447	regression	1438:1447	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	3	105	theme	age	494:496	arg1	influence					382:390	the influence	378:390	the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves	378:543	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	14	106	with	calves	2073:2078	arg1	trials					2088:2093	2 trials	2086:2093	2 trials	2086:2093	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	11	107	from	variation	1755:1763	arg1	TTSD					1768:1771	TTSD	1768:1771	TTSD	1768:1771	Prior to weaning, SI, age, and MR rate explained 89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39).
28571991	5	108	contain	contained	873:881	arg1	composition					785:795	Starter ingredient composition	766:795	Starter ingredient composition	766:795	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	5	108	contain	contained	873:881	arg2	starch					893:898	43 ± 1.9% starch	883:898	43 ± 1.9% starch	883:898	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	11	109	theme	MR	1726:1727	arg1	rate					1729:1732	MR rate	1726:1732	MR rate	1726:1732	Prior to weaning, SI, age, and MR rate explained 89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39).
28571991	14	110	theme	MR	2101:2102	arg1	rate					2104:2107	MR rate	2101:2107	MR rate	2101:2107	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	6	111	theme	=	951:951	arg1	n					949:949	n = 20	949:954	n = 20	949:954	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	6	111	theme	=	951:951	arg1	6					946:946	6	946:946	6	946:946	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	5	112	theme	whole	821:825	arg1	oats					827:830	whole oats	821:830	20% whole oats	817:830	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	11	113	from	TTSD	1768:1771	arg1	%					1746:1746	89%	1744:1746	89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39)	1744:1864	Prior to weaning, SI, age, and MR rate explained 89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39).
28571991	11	113	from	TTSD	1768:1771	arg1	variation					1755:1763	the variation	1751:1763	the variation	1751:1763	Prior to weaning, SI, age, and MR rate explained 89% of the variation in TTSD, where TTSD = [19.7 × SI (±4.25)] + [3.8 × age (±0.79)] - [24.8 × MR (±3.19)] + 56.2 (±3.39).
28571991	6	114	theme	grab	907:910	arg1	samples					912:918	Fecal grab samples	901:918	Fecal grab samples	901:918	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	4	115	theme	body	583:586	arg1	weight					588:593	body weight	583:593	body weight	583:593	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	8	116	used	used	1279:1282	arg2	ash					1271:1273	acid-insoluble ash	1256:1273	acid-insoluble ash	1256:1273	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	8	116	used	used	1279:1282	arg2	marker					1299:1304	an internal marker	1287:1304	an internal marker at 8 wk	1287:1312	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	10	117	theme	total-tract	1492:1502	arg1	TTSD					1526:1529	TTSD	1526:1529	TTSD	1526:1529	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	117	theme	total-tract	1492:1502	arg1	digestibility					1511:1523	total-tract starch digestibility	1492:1523	total-tract starch digestibility (TTSD; % of DM)	1492:1539	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	3	118	theme	FS	501:502	arg1	concentrations					504:517	FS concentrations	501:517	FS concentrations in preweaned dairy calves	501:543	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	1	119	theme	Fecal	120:124	arg1	starch					126:131	Fecal starch	120:131	Fecal starch (FS)	120:136	Fecal starch (FS) has been used as a tool to evaluate starch and diet digestibility in lactating dairy cows and feedlot steers.
28571991	1	119	theme	Fecal	120:124	arg1	tool					157:160	a tool	155:160	a tool to evaluate starch and diet digestibility in lactating dairy cows and feedlot steers	155:245	Fecal starch (FS) has been used as a tool to evaluate starch and diet digestibility in lactating dairy cows and feedlot steers.
28571991	1	119	theme	Fecal	120:124	arg1	FS					134:135	FS	134:135	FS	134:135	Fecal starch (FS) has been used as a tool to evaluate starch and diet digestibility in lactating dairy cows and feedlot steers.
28571991	14	120	theme	×	2246:2246	arg1	trial					2248:2252	[1.1 × trial	2241:2252	[1.1 × trial	2241:2252	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	15	121	dep	increased	2323:2331	arg1	coefficient					2334:2344	coefficient	2334:2344	coefficient of determination = 0.86	2334:2368	Postweaning, TTSD decreased linearly as FS increased (coefficient of determination = 0.86), whereas FS and SI were unrelated, a relationship in contrast to the previously observed result in calves still consuming milk replacer.
28571991	16	122	from	good	2543:2546	arg1	study					2523:2527	the current study	2511:2527	the current study	2511:2527	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	16	122	from	good	2543:2546	arg1	calf					2601:2604	the preweaned calf	2587:2604	the preweaned calf	2587:2604	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	4	123	dep	daily	704:708	arg1	%					732:732	17% fat	730:736	17% fat	730:736	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	123	dep	daily	704:708	arg1	%					713:713	27%	711:713	27% crude protein	711:727	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	15	124	from	result	2460:2465	arg1	calves					2470:2475	calves	2470:2475	calves still consuming milk replacer	2470:2505	Postweaning, TTSD decreased linearly as FS increased (coefficient of determination = 0.86), whereas FS and SI were unrelated, a relationship in contrast to the previously observed result in calves still consuming milk replacer.
28571991	16	125	theme	feed	2572:2575	arg1	intake					2577:2582	dry feed intake	2568:2582	dry feed intake	2568:2582	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	7	126	theme	wet-weight	1110:1119	arg1	basis					1121:1125	an equal wet-weight basis	1101:1125	an equal wet-weight basis	1101:1125	Twelve fecal samples per calf were taken via rectal palpation over a 5-d period each week, frozen daily, combined on an equal wet-weight basis, and subsampled for analysis.
28571991	9	127	dep	replacer	1320:1327	arg1	refused					1362:1368	refused	1362:1368	refused	1362:1368	Milk replacer and starter intakes (offered and refused) were recorded daily during collection periods.
28571991	9	127	dep	replacer	1320:1327	arg1	offered					1350:1356	offered	1350:1356	offered	1350:1356	Milk replacer and starter intakes (offered and refused) were recorded daily during collection periods.
28571991	10	128	theme	DM	1608:1609	arg1	%					1597:1597	%	1597:1597	% of fecal DM	1597:1609	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	128	theme	DM	1608:1609	arg1	DM					1608:1609	fecal DM	1602:1609	fecal DM	1602:1609	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	14	129	theme	8-wk-old	2064:2071	arg1	calves					2073:2078	8-wk-old calves	2064:2078	8-wk-old calves (with 2 trials)	2064:2094	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	0	130	theme	feed	78:81	arg1	digestion					83:91	dry feed digestion	74:91	dry feed digestion in preweaned dairy calves	74:117	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	4	131	from	farm	618:621	arg1	calves					560:565	Male Holstein calves	546:565	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm	546:621	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	14	132	dep	%	2136:2136	arg1	FS					2227:2228	[0.6 × FS (±0.04)	2220:2236	[0.6 × FS (±0.04)	2220:2236	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	14	132	dep	%	2136:2136	arg1	MR					2206:2207	[2.4 × MR (±0.56)	2199:2215	[2.4 × MR (±0.56)	2199:2215	In 8-wk-old calves (with 2 trials), SI, MR rate, FS, and trial explained 92% of the variation in TTSD, where TTSD = -[2.6 × SI (±0.67)] - [2.4 × MR (±0.56)] - [0.6 × FS (±0.04)] + [1.1 × trial (±0.33)] + 100.4 (±1.02).
28571991	10	133	theme	digestibility	1511:1523	arg1	regression					1438:1447	Multiple and linear regression	1418:1447	regression	1438:1447	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	16	134	theme	preweaned	2591:2599	arg1	calf					2601:2604	the preweaned calf	2587:2604	the preweaned calf	2587:2604	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	15	135	theme	=	2363:2363	arg1	coefficient					2334:2344	coefficient	2334:2344	coefficient of determination = 0.86	2334:2368	Postweaning, TTSD decreased linearly as FS increased (coefficient of determination = 0.86), whereas FS and SI were unrelated, a relationship in contrast to the previously observed result in calves still consuming milk replacer.
28571991	4	136	theme	fat	734:736	arg1	%					732:732	17% fat	730:736	17% fat	730:736	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	136	theme	fat	734:736	arg1	%					713:713	27%	711:713	27% crude protein	711:727	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	10	137	dep	digestibility	1467:1479	arg1	%					1482:1482	%	1482:1482	% of DM	1482:1488	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	137	dep	digestibility	1467:1479	arg1	DM					1487:1488	DM	1487:1488	DM	1487:1488	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	2	138	theme	dairy	334:338	arg1	calves					340:345	preweaned dairy calves	324:345	preweaned dairy calves	324:345	Some on-farm advisors also use FS to evaluate calf starter digestibility in preweaned dairy calves.
28571991	10	139	dep	FS	1593:1594	arg1	%					1597:1597	%	1597:1597	% of fecal DM	1597:1609	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	139	dep	FS	1593:1594	arg1	DM					1608:1609	fecal DM	1602:1609	fecal DM	1602:1609	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	16	140	theme	current	2515:2521	arg1	study					2523:2527	the current study	2511:2527	the current study	2511:2527	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	4	141	dep	1.10	677:680	arg1	to					674:675	to	674:675	to	674:675	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	0	142	dep	communication	6:18	arg1	Use					21:23	Use	21:23	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.	0:118	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	1	143	used	used	147:150	arg2	FS					134:135	FS	134:135	FS	134:135	Fecal starch (FS) has been used as a tool to evaluate starch and diet digestibility in lactating dairy cows and feedlot steers.
28571991	1	143	used	used	147:150	arg2	starch					126:131	Fecal starch	120:131	Fecal starch (FS)	120:136	Fecal starch (FS) has been used as a tool to evaluate starch and diet digestibility in lactating dairy cows and feedlot steers.
28571991	1	143	used	used	147:150	arg2	tool					157:160	a tool	155:160	a tool to evaluate starch and diet digestibility in lactating dairy cows and feedlot steers	155:245	Fecal starch (FS) has been used as a tool to evaluate starch and diet digestibility in lactating dairy cows and feedlot steers.
28571991	9	144	theme	Milk	1315:1318	arg1	replacer					1320:1327	Milk replacer and starter intakes	1315:1347	replacer	1320:1327	Milk replacer and starter intakes (offered and refused) were recorded daily during collection periods.
28571991	16	145	from	calf	2601:2604	arg1	good					2543:2546	good	2543:2546	good	2543:2546	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	16	145	from	calf	2601:2604	arg1	FS					2530:2531	FS	2530:2531	FS	2530:2531	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	0	146	theme	fecal	28:32	arg1	concentration					41:53	fecal starch concentration	28:53	fecal starch concentration	28:53	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	7	147	dep	period	1057:1062	arg1	week					1069:1072	each week	1064:1072	a 5-d period each week	1051:1072	Twelve fecal samples per calf were taken via rectal palpation over a 5-d period each week, frozen daily, combined on an equal wet-weight basis, and subsampled for analysis.
28571991	5	148	theme	Starter	766:772	arg1	composition					785:795	Starter ingredient composition	766:795	Starter ingredient composition	766:795	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	12	149	theme	determination	1914:1926	arg1	coefficient					1899:1909	coefficient	1899:1909	coefficient of determination	1899:1926	At 3 wk of age, TTSD increased (coefficient of determination = 0.53) and SI decreased (coefficient of determination = 0.20) with increasing FS.
28571991	3	150	theme	dairy	532:536	arg1	calves					538:543	preweaned dairy calves	522:543	preweaned dairy calves	522:543	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	4	151	theme	MR	653:654	arg1	amounts					642:648	different amounts	632:648	different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat)	632:737	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	151	theme	MR	653:654	arg1	matter					692:697	dry matter	688:697	dry matter (DM)	688:702	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	151	theme	MR	653:654	arg1	MR					653:654	MR	653:654	MR ranging from 0.44 to 1.10 kg	653:683	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	4	151	theme	MR	653:654	arg1	DM					700:701	DM	700:701	DM	700:701	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	6	152	theme	8	962:962	arg1	n					968:968	n = 35	968:973	n = 35	968:973	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	6	152	theme	8	962:962	arg1	wk					964:965	3 (n = 20), 6 (n = 20), and 8 wk	934:965	3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age	934:981	Fecal grab samples were taken at 3 (n = 20), 6 (n = 20), and 8 wk (n = 35) of age.
28571991	0	153	theme	concentration	41:53	arg1	Use					21:23	Use	21:23	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.	0:118	Short communication: Use of fecal starch concentration as an indicator of dry feed digestion in preweaned dairy calves.
28571991	16	154	from	TTSD	2640:2643	arg1	calves					2648:2653	calves	2648:2653	calves	2648:2653	In the current study, FS was not a good estimate of TTSD or dry feed intake in the preweaned calf, but has potential for evaluating TTSD in calves after weaning.
28571991	4	155	theme	age	761:763	arg1	wk					755:756	7 wk	753:756	7 wk of age	753:763	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	3	156	theme	starch	416:421	arg1	digestibility					442:454	starch and organic matter digestibility	416:454	starch and organic matter digestibility	416:454	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
28571991	8	157	theme	digestibility	1195:1207	arg1	marker					1209:1214	an external digestibility marker	1183:1214	an external digestibility marker	1183:1214	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	8	157	theme	digestibility	1195:1207	arg1	oxide					1165:1169	Chromic oxide	1157:1169	Chromic oxide	1157:1169	Chromic oxide was used as an external digestibility marker at 3 and 6 wk (included in MR), whereas acid-insoluble ash was used as an internal marker at 8 wk.
28571991	10	158	theme	DM	1537:1538	arg1	DM					1537:1538	DM	1537:1538	DM	1537:1538	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	10	158	theme	DM	1537:1538	arg1	%					1532:1532	%	1532:1532	% of DM	1532:1538	Multiple and linear regression of organic matter digestibility (% of DM), total-tract starch digestibility (TTSD; % of DM), MR intake (kg/d), SI (kg/d), and age (week) versus FS (% of fecal DM) were determined using PROC REG of SAS (version 9.2, SAS Institute Inc., Cary, NC).
28571991	5	159	dep	%	819:819	arg1	oats					827:830	whole oats	821:830	20% whole oats	817:830	Starter ingredient composition was 37% whole corn, 20% whole oats, 35% protein pellet, and 3% molasses and contained 43 ± 1.9% starch.
28571991	2	160	theme	starter	299:305	arg1	digestibility					307:319	calf starter digestibility	294:319	calf starter digestibility in preweaned dairy calves	294:345	Some on-farm advisors also use FS to evaluate calf starter digestibility in preweaned dairy calves.
28571991	4	161	dep	calves	560:565	arg1	=					598:598	=	598:598	=	598:598	Male Holstein calves (43 ± 2.9 kg of body weight; n = 35) from a single farm were fed different amounts of MR ranging from 0.44 to 1.10 kg of dry matter (DM) daily (27% crude protein, 17% fat) and weaned by 7 wk of age.
28571991	3	162	theme	digestibility	442:454	arg1	influence					382:390	the influence	378:390	the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves	378:543	Our objective was to evaluate the influence of starter intake (SI), starch and organic matter digestibility, milk replacer (MR) feeding rate, and age on FS concentrations in preweaned dairy calves.
27993213	10	0	theme	warm	1825:1828	arg1	reperfusion					1830:1840	warm reperfusion	1825:1840	warm reperfusion	1825:1840	CONCLUSIONS We conclude that HMP with modified AL solution showed improved perfusion compared with UW and lower perfusate lactate levels during warm reperfusion.
27993213	11	1	theme	AL	1871:1872	arg1	composition					1874:1884	the AL composition	1867:1884	the AL composition	1867:1884	Further modification of the AL composition is warranted and may lead to more rapid kidney stabilization and improved graft viability assessment, potentially expanding donor pools.
27993213	3	2	theme	kidney	482:487	arg1	preservation					489:500	kidney preservation	482:500	kidney preservation compared with University Wisconsin solution (UW)	482:549	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	7	3	theme	creatinine	1354:1363	arg1	clearance					1365:1373	creatinine clearance	1354:1373	creatinine clearance	1354:1373	During warm reperfusion, there were no significant differences between the two HMP groups in creatinine clearance, oxygen, and glucose consumption between groups.
27993213	4	4	theme	death	600:604	arg1	kidneys					614:620	cardiac death porcine kidneys	592:620	cardiac death porcine kidneys	592:620	METHODS Twenty donation of organs after cardiac death porcine kidneys underwent HMP for 10 h (AL, n = 10; UW, n = 10) and then 2 h of normothermic reperfusion.
27993213	11	5	theme	rapid	1920:1924	arg1	stabilization					1933:1945	more rapid kidney stabilization	1915:1945	more rapid kidney stabilization	1915:1945	Further modification of the AL composition is warranted and may lead to more rapid kidney stabilization and improved graft viability assessment, potentially expanding donor pools.
27993213	5	6	theme	real-time	770:778	arg1	microdialysis					780:792	real-time microdialysis	770:792	real-time microdialysis	770:792	Perfusion dynamics, functional parameters, histology, and real-time microdialysis were used to assess kidney responses and viability.
27993213	6	7	dep	AL = 0.50 ± 0.2	1040:1054	arg1	UW = 0.40 ± 0.17 mL/min/100 g/mmHg					1057:1090	UW = 0.40 ± 0.17 mL/min/100 g/mmHg	1057:1090	UW = 0.40 ± 0.17 mL/min/100 g/mmHg	1057:1090	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	6	7	dep	AL = 0.50 ± 0.2	1040:1054	arg1	P = 0.03					1093:1100	P = 0.03	1093:1100	P = 0.03	1093:1100	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	3	8	theme	University	516:525	arg1	UW					547:548	UW	547:548	UW	547:548	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	3	8	theme	University	516:525	arg1	solution					537:544	University Wisconsin solution	516:544	University Wisconsin solution (UW)	516:549	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	1	9	theme	optimal	191:197	arg1	solution					235:242	The optimal hypothermic machine perfusion (HMP) solution	187:242	The optimal hypothermic machine perfusion (HMP) solution	187:242	BACKGROUND The optimal hypothermic machine perfusion (HMP) solution has not yet been developed.
27993213	6	10	dep	7 h	1205:1207	arg1	0.54					1210:1213	0.54	1210:1213	0.54	1210:1213	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	6	10	dep	7 h	1205:1207	arg1	0.55 mL/min/100 g/mmHg					1222:1243	0.55 mL/min/100 g/mmHg	1222:1243	0.55 mL/min/100 g/mmHg	1222:1243	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	10	11	with	HMP	1710:1712	arg1	solution					1731:1738	modified AL solution	1719:1738	modified AL solution	1719:1738	CONCLUSIONS We conclude that HMP with modified AL solution showed improved perfusion compared with UW and lower perfusate lactate levels during warm reperfusion.
27993213	2	12	from	protective	334:343	arg1	preservation					356:367	cardiac preservation	348:367	cardiac preservation	348:367	An adenosine and lidocaine (AL) solution has been shown to be protective in cardiac preservation.
27993213	8	13	dep	3.1	1488:1490	arg1	P = 0.04					1511:1518	P = 0.04	1511:1518	P = 0.04	1511:1518	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	2	14	theme	lidocaine	289:297	arg1	solution					304:311	An adenosine and lidocaine (AL) solution	272:311	An adenosine and lidocaine (AL) solution	272:311	An adenosine and lidocaine (AL) solution has been shown to be protective in cardiac preservation.
27993213	2	14	theme	lidocaine	289:297	arg1	protective					334:343	protective	334:343	protective	334:343	An adenosine and lidocaine (AL) solution has been shown to be protective in cardiac preservation.
27993213	1	15	theme	machine	211:217	arg1	solution					235:242	The optimal hypothermic machine perfusion (HMP) solution	187:242	The optimal hypothermic machine perfusion (HMP) solution	187:242	BACKGROUND The optimal hypothermic machine perfusion (HMP) solution has not yet been developed.
27993213	6	16	theme	modified	866:873	arg1	kidneys					887:893	modified AL-perfused kidneys	866:893	modified AL-perfused kidneys	866:893	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	6	17	dep	RESULTS	846:852	arg1	maintained					895:904	maintained	895:904	maintained	895:904	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	6	18	theme	UW	1131:1132	arg1	kidneys					1134:1140	UW kidneys	1131:1140	UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively)	1131:1258	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	0	19	theme	porcine	132:138	arg1	kidneys					140:146	porcine kidneys	132:146	porcine kidneys	132:146	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.
27993213	10	20	theme	modified	1719:1726	arg1	solution					1731:1738	modified AL solution	1719:1738	modified AL solution	1719:1738	CONCLUSIONS We conclude that HMP with modified AL solution showed improved perfusion compared with UW and lower perfusate lactate levels during warm reperfusion.
27993213	11	21	theme	donor	2010:2014	arg1	pools					2016:2020	donor pools	2010:2020	donor pools	2010:2020	Further modification of the AL composition is warranted and may lead to more rapid kidney stabilization and improved graft viability assessment, potentially expanding donor pools.
27993213	6	22	theme	remaining	1195:1203	arg1	7 h					1205:1207	the remaining 7 h	1191:1207	the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively)	1191:1258	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	5	23	used	used	799:802	arg2	parameters					743:752	functional parameters	732:752	functional parameters	732:752	Perfusion dynamics, functional parameters, histology, and real-time microdialysis were used to assess kidney responses and viability.
27993213	5	23	used	used	799:802	arg2	dynamics					722:729	Perfusion dynamics	712:729	Perfusion dynamics	712:729	Perfusion dynamics, functional parameters, histology, and real-time microdialysis were used to assess kidney responses and viability.
27993213	5	23	used	used	799:802	arg2	microdialysis					780:792	real-time microdialysis	770:792	real-time microdialysis	770:792	Perfusion dynamics, functional parameters, histology, and real-time microdialysis were used to assess kidney responses and viability.
27993213	5	23	used	used	799:802	arg2	histology					755:763	histology	755:763	histology	755:763	Perfusion dynamics, functional parameters, histology, and real-time microdialysis were used to assess kidney responses and viability.
27993213	9	24	theme	similar	1642:1648	arg1	degrees					1650:1656	similar degrees	1642:1656	similar degrees of reperfusion injury	1642:1678	Histology showed similar degrees of reperfusion injury.
27993213	8	25	dep	had	1444:1446	arg1	4.1 mmol/L					1499:1508	4.1 mmol/L	1499:1508	4.1 mmol/L	1499:1508	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	8	25	dep	had	1444:1446	arg1	3.1					1488:1490	3.1	1488:1490	3.1	1488:1490	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	10	26	theme	improved	1747:1754	arg1	perfusion					1756:1764	improved perfusion	1747:1764	improved perfusion	1747:1764	CONCLUSIONS We conclude that HMP with modified AL solution showed improved perfusion compared with UW and lower perfusate lactate levels during warm reperfusion.
27993213	11	27	dep	stabilization	1933:1945	arg1	assessment					1976:1985	assessment	1976:1985	assessment	1976:1985	Further modification of the AL composition is warranted and may lead to more rapid kidney stabilization and improved graft viability assessment, potentially expanding donor pools.
27993213	9	28	theme	injury	1673:1678	arg1	degrees					1650:1656	similar degrees	1642:1656	similar degrees of reperfusion injury	1642:1678	Histology showed similar degrees of reperfusion injury.
27993213	8	29	contain	had	1444:1446	arg1	kidneys					1436:1442	AL kidneys	1433:1442	Modified AL kidneys	1424:1442	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	8	29	contain	had	1444:1446	arg2	lactates					1478:1485	significantly lower perfusate lactates	1448:1485	significantly lower perfusate lactates	1448:1485	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	5	30	theme	kidney	814:819	arg1	responses					821:829	kidney responses	814:829	kidney responses	814:829	Perfusion dynamics, functional parameters, histology, and real-time microdialysis were used to assess kidney responses and viability.
27993213	6	31	dep	rates	918:922	arg1	P = 0.01					956:963	P = 0.01	956:963	P = 0.01	956:963	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	6	31	dep	rates	918:922	arg1	g					953:953	21.5 versus 17.9 mL/min/100 g	925:953	21.5 versus 17.9 mL/min/100 g	925:953	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	0	32	theme	solution	86:93	arg1	comparison					11:20	An ex vivo comparison	0:20	An ex vivo comparison of adenosine and lidocaine solution	0:56	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.
27993213	2	33	theme	adenosine	275:283	arg1	solution					304:311	An adenosine and lidocaine (AL) solution	272:311	An adenosine and lidocaine (AL) solution	272:311	An adenosine and lidocaine (AL) solution has been shown to be protective in cardiac preservation.
27993213	2	33	theme	adenosine	275:283	arg1	protective					334:343	protective	334:343	protective	334:343	An adenosine and lidocaine (AL) solution has been shown to be protective in cardiac preservation.
27993213	6	34	theme	higher	906:911	arg1	rates					918:922	higher flow rates	906:922	higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01)	906:964	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	0	35	theme	hypothermic	99:109	arg1	perfusion					119:127	hypothermic machine perfusion	99:127	hypothermic machine perfusion of porcine kidneys	99:146	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.
27993213	4	36	dep	underwent	622:630	arg1	n = 10					650:655	n = 10	650:655	n = 10	650:655	METHODS Twenty donation of organs after cardiac death porcine kidneys underwent HMP for 10 h (AL, n = 10; UW, n = 10) and then 2 h of normothermic reperfusion.
27993213	6	37	theme	flow	982:985	arg1	index					987:991	perfusion flow index	972:991	perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03)	972:1101	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	8	38	theme	Modified	1424:1431	arg1	kidneys					1436:1442	AL kidneys	1433:1442	Modified AL kidneys	1424:1442	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	3	39	theme	study	393:397	arg1	aim					374:376	The aim	370:376	The aim of the present study	370:397	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	11	40	theme	composition	1874:1884	arg1	modification					1851:1862	Further modification	1843:1862	Further modification of the AL composition	1843:1884	Further modification of the AL composition is warranted and may lead to more rapid kidney stabilization and improved graft viability assessment, potentially expanding donor pools.
27993213	3	41	theme	AL	425:426	arg1	solution					428:435	a modified AL solution	414:435	a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW)	414:549	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	5	42	theme	functional	732:741	arg1	parameters					743:752	functional parameters	732:752	functional parameters	732:752	Perfusion dynamics, functional parameters, histology, and real-time microdialysis were used to assess kidney responses and viability.
27993213	11	43	theme	Further	1843:1849	arg1	modification					1851:1862	Further modification	1843:1862	Further modification of the AL composition	1843:1884	Further modification of the AL composition is warranted and may lead to more rapid kidney stabilization and improved graft viability assessment, potentially expanding donor pools.
27993213	0	44	theme	adenosine	25:33	arg1	comparison					11:20	An ex vivo comparison	0:20	An ex vivo comparison of adenosine and lidocaine solution	0:56	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.
27993213	2	45	from	preservation	356:367	arg1	solution					304:311	An adenosine and lidocaine (AL) solution	272:311	An adenosine and lidocaine (AL) solution	272:311	An adenosine and lidocaine (AL) solution has been shown to be protective in cardiac preservation.
27993213	2	45	from	preservation	356:367	arg1	protective					334:343	protective	334:343	protective	334:343	An adenosine and lidocaine (AL) solution has been shown to be protective in cardiac preservation.
27993213	8	46	theme	perfusate	1468:1476	arg1	lactates					1478:1485	significantly lower perfusate lactates	1448:1485	significantly lower perfusate lactates	1448:1485	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	0	47	theme	lidocaine	39:47	arg1	comparison					11:20	An ex vivo comparison	0:20	An ex vivo comparison of adenosine and lidocaine solution	0:56	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.
27993213	0	48	dep	adenosine	25:33	arg1	solution					49:56	solution	49:56	solution	49:56	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.
27993213	6	49	theme	greater	1018:1024	arg1	3 h					1010:1012	the first 3 h	1000:1012	the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03)	1000:1101	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	10	50	theme	lactate	1803:1809	arg1	levels					1811:1816	lower perfusate lactate levels	1787:1816	lower perfusate lactate levels	1787:1816	CONCLUSIONS We conclude that HMP with modified AL solution showed improved perfusion compared with UW and lower perfusate lactate levels during warm reperfusion.
27993213	4	51	theme	normothermic	686:697	arg1	reperfusion					699:709	normothermic reperfusion	686:709	normothermic reperfusion	686:709	METHODS Twenty donation of organs after cardiac death porcine kidneys underwent HMP for 10 h (AL, n = 10; UW, n = 10) and then 2 h of normothermic reperfusion.
27993213	3	52	from	solution	428:435	arg1	preservation					489:500	kidney preservation	482:500	kidney preservation compared with University Wisconsin solution (UW)	482:549	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	8	53	theme	lower	1544:1548	arg1	levels					1567:1572	lower cortical lactate levels	1544:1572	lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33)	1544:1622	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	1	54	theme	HMP	230:232	arg1	solution					235:242	The optimal hypothermic machine perfusion (HMP) solution	187:242	The optimal hypothermic machine perfusion (HMP) solution	187:242	BACKGROUND The optimal hypothermic machine perfusion (HMP) solution has not yet been developed.
27993213	0	55	dep	comparison	11:20	arg1	potential					149:157	potential	149:157	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.	0:174	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.
27993213	10	56	theme	lower	1787:1791	arg1	levels					1811:1816	lower perfusate lactate levels	1787:1816	lower perfusate lactate levels	1787:1816	CONCLUSIONS We conclude that HMP with modified AL solution showed improved perfusion compared with UW and lower perfusate lactate levels during warm reperfusion.
27993213	8	57	theme	lactate	1559:1565	arg1	levels					1567:1572	lower cortical lactate levels	1544:1572	lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33)	1544:1622	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	6	58	theme	first	1004:1008	arg1	3 h					1010:1012	the first 3 h	1000:1012	the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03)	1000:1101	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	11	59	theme	kidney	1926:1931	arg1	stabilization					1933:1945	more rapid kidney stabilization	1915:1945	more rapid kidney stabilization	1915:1945	Further modification of the AL composition is warranted and may lead to more rapid kidney stabilization and improved graft viability assessment, potentially expanding donor pools.
27993213	3	60	theme	%	469:469	arg1	albumin					471:477	4% albumin	468:477	4% albumin	468:477	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	7	61	theme	HMP	1340:1342	arg1	groups					1344:1349	the two HMP groups	1332:1349	the two HMP groups in creatinine clearance, oxygen, and glucose consumption	1332:1406	During warm reperfusion, there were no significant differences between the two HMP groups in creatinine clearance, oxygen, and glucose consumption between groups.
27993213	4	62	theme	cardiac	592:598	arg1	kidneys					614:620	cardiac death porcine kidneys	592:620	cardiac death porcine kidneys	592:620	METHODS Twenty donation of organs after cardiac death porcine kidneys underwent HMP for 10 h (AL, n = 10; UW, n = 10) and then 2 h of normothermic reperfusion.
27993213	11	63	theme	graft	1960:1964	arg1	viability					1966:1974	improved graft viability	1951:1974	improved graft viability	1951:1974	Further modification of the AL composition is warranted and may lead to more rapid kidney stabilization and improved graft viability assessment, potentially expanding donor pools.
27993213	7	64	from	groups	1344:1349	arg1	consumption					1396:1406	glucose consumption	1388:1406	glucose consumption	1388:1406	During warm reperfusion, there were no significant differences between the two HMP groups in creatinine clearance, oxygen, and glucose consumption between groups.
27993213	7	64	from	groups	1344:1349	arg1	oxygen					1376:1381	oxygen	1376:1381	oxygen	1376:1381	During warm reperfusion, there were no significant differences between the two HMP groups in creatinine clearance, oxygen, and glucose consumption between groups.
27993213	7	64	from	groups	1344:1349	arg1	clearance					1365:1373	creatinine clearance	1354:1373	creatinine clearance	1354:1373	During warm reperfusion, there were no significant differences between the two HMP groups in creatinine clearance, oxygen, and glucose consumption between groups.
27993213	7	65	theme	significant	1300:1310	arg1	differences					1312:1322	no significant differences	1297:1322	no significant differences between the two HMP groups in creatinine clearance, oxygen, and glucose consumption between groups	1297:1421	During warm reperfusion, there were no significant differences between the two HMP groups in creatinine clearance, oxygen, and glucose consumption between groups.
27993213	4	66	theme	porcine	606:612	arg1	kidneys					614:620	cardiac death porcine kidneys	592:620	cardiac death porcine kidneys	592:620	METHODS Twenty donation of organs after cardiac death porcine kidneys underwent HMP for 10 h (AL, n = 10; UW, n = 10) and then 2 h of normothermic reperfusion.
27993213	1	67	theme	hypothermic	199:209	arg1	solution					235:242	The optimal hypothermic machine perfusion (HMP) solution	187:242	The optimal hypothermic machine perfusion (HMP) solution	187:242	BACKGROUND The optimal hypothermic machine perfusion (HMP) solution has not yet been developed.
27993213	6	68	theme	AL-perfused	875:885	arg1	kidneys					887:893	modified AL-perfused kidneys	866:893	modified AL-perfused kidneys	866:893	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	0	69	theme	kidneys	140:146	arg1	perfusion					119:127	hypothermic machine perfusion	99:127	hypothermic machine perfusion of porcine kidneys	99:146	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.
27993213	10	70	theme	AL	1728:1729	arg1	solution					1731:1738	modified AL solution	1719:1738	modified AL solution	1719:1738	CONCLUSIONS We conclude that HMP with modified AL solution showed improved perfusion compared with UW and lower perfusate lactate levels during warm reperfusion.
27993213	1	71	theme	perfusion	219:227	arg1	solution					235:242	The optimal hypothermic machine perfusion (HMP) solution	187:242	The optimal hypothermic machine perfusion (HMP) solution	187:242	BACKGROUND The optimal hypothermic machine perfusion (HMP) solution has not yet been developed.
27993213	4	72	theme	organs	579:584	arg1	donation					567:574	Twenty donation	560:574	Twenty donation of organs	560:584	METHODS Twenty donation of organs after cardiac death porcine kidneys underwent HMP for 10 h (AL, n = 10; UW, n = 10) and then 2 h of normothermic reperfusion.
27993213	3	73	theme	Wisconsin	527:535	arg1	UW					547:548	UW	547:548	UW	547:548	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	3	73	theme	Wisconsin	527:535	arg1	solution					537:544	University Wisconsin solution	516:544	University Wisconsin solution (UW)	516:549	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	7	74	theme	warm	1268:1271	arg1	reperfusion					1273:1283	warm reperfusion	1268:1283	warm reperfusion	1268:1283	During warm reperfusion, there were no significant differences between the two HMP groups in creatinine clearance, oxygen, and glucose consumption between groups.
27993213	10	75	dep	CONCLUSIONS	1681:1691	arg1	conclude					1696:1703	conclude	1696:1703	conclude that HMP with modified AL solution showed improved perfusion compared with UW and lower perfusate lactate levels during warm reperfusion	1696:1840	CONCLUSIONS We conclude that HMP with modified AL solution showed improved perfusion compared with UW and lower perfusate lactate levels during warm reperfusion.
27993213	6	76	from	increase	1119:1126	arg1	kidneys					1134:1140	UW kidneys	1131:1140	UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively)	1131:1258	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	8	77	dep	levels	1567:1572	arg1	AL = 0.66 ± 0.31					1575:1590	AL = 0.66 ± 0.31	1575:1590	AL = 0.66 ± 0.31	1575:1590	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	8	77	dep	levels	1567:1572	arg1	P = 0.33					1614:1621	P = 0.33	1614:1621	P = 0.33	1614:1621	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	8	77	dep	levels	1567:1572	arg1	UW = 0.89 ± 0.53 mM					1593:1611	UW = 0.89 ± 0.53 mM	1593:1611	UW = 0.89 ± 0.53 mM	1593:1611	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	9	78	theme	reperfusion	1661:1671	arg1	injury					1673:1678	reperfusion injury	1661:1678	reperfusion injury	1661:1678	Histology showed similar degrees of reperfusion injury.
27993213	0	79	theme	Wisconsin	76:84	arg1	solution					86:93	Wisconsin solution	76:93	Wisconsin solution for hypothermic machine perfusion of porcine kidneys	76:146	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.
27993213	2	80	dep	adenosine	275:283	arg1	AL					300:301	AL	300:301	AL	300:301	An adenosine and lidocaine (AL) solution has been shown to be protective in cardiac preservation.
27993213	11	81	theme	improved	1951:1958	arg1	viability					1966:1974	improved graft viability	1951:1974	improved graft viability	1951:1974	Further modification of the AL composition is warranted and may lead to more rapid kidney stabilization and improved graft viability assessment, potentially expanding donor pools.
27993213	6	82	theme	flow	913:916	arg1	rates					918:922	higher flow rates	906:922	higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01)	906:964	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	0	83	theme	machine	111:117	arg1	perfusion					119:127	hypothermic machine perfusion	99:127	hypothermic machine perfusion of porcine kidneys	99:146	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.
27993213	8	84	theme	AL	1433:1434	arg1	kidneys					1436:1442	AL kidneys	1433:1442	Modified AL kidneys	1424:1442	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	5	85	theme	Perfusion	712:720	arg1	dynamics					722:729	Perfusion dynamics	712:729	Perfusion dynamics	712:729	Perfusion dynamics, functional parameters, histology, and real-time microdialysis were used to assess kidney responses and viability.
27993213	4	86	dep	n = 10	650:655	arg1	AL					646:647	AL	646:647	AL	646:647	METHODS Twenty donation of organs after cardiac death porcine kidneys underwent HMP for 10 h (AL, n = 10; UW, n = 10) and then 2 h of normothermic reperfusion.
27993213	4	86	dep	n = 10	650:655	arg1	n = 10					662:667	n = 10	662:667	n = 10	662:667	METHODS Twenty donation of organs after cardiac death porcine kidneys underwent HMP for 10 h (AL, n = 10; UW, n = 10) and then 2 h of normothermic reperfusion.
27993213	4	86	dep	n = 10	650:655	arg1	UW					658:659	UW	658:659	UW	658:659	METHODS Twenty donation of organs after cardiac death porcine kidneys underwent HMP for 10 h (AL, n = 10; UW, n = 10) and then 2 h of normothermic reperfusion.
27993213	6	87	theme	perfusion	972:980	arg1	index					987:991	perfusion flow index	972:991	perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03)	972:1101	RESULTS During HMP, modified AL-perfused kidneys maintained higher flow rates (21.5 versus 17.9 mL/min/100 g, P = 0.01), with perfusion flow index during the first 3 h 25% greater than with UW (AL = 0.50 ± 0.2, UW = 0.40 ± 0.17 mL/min/100 g/mmHg; P = 0.03), followed by an increase in UW kidneys which was not significantly different to AL over the remaining 7 h (0.54 versus 0.55 mL/min/100 g/mmHg, respectively).
27993213	2	88	theme	cardiac	348:354	arg1	preservation					356:367	cardiac preservation	348:367	cardiac preservation	348:367	An adenosine and lidocaine (AL) solution has been shown to be protective in cardiac preservation.
27993213	8	89	theme	lower	1462:1466	arg1	lactates					1478:1485	significantly lower perfusate lactates	1448:1485	significantly lower perfusate lactates	1448:1485	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	3	90	theme	present	385:391	arg1	study					393:397	the present study	381:397	the present study	381:397	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	3	91	theme	modified	416:423	arg1	solution					428:435	a modified AL solution	414:435	a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW)	414:549	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	3	92	with	solution	428:435	arg1	Ca2+					446:449	low Ca2+	442:449	low Ca2+	442:449	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	3	92	with	solution	428:435	arg1	albumin					471:477	4% albumin	468:477	4% albumin	468:477	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	3	92	with	solution	428:435	arg1	16 mM Mg2+					452:461	16 mM Mg2+	452:461	16 mM Mg2+	452:461	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	1	93	dep	BACKGROUND	176:185	arg1	developed					261:269	developed	261:269	has not yet been developed	244:269	BACKGROUND The optimal hypothermic machine perfusion (HMP) solution has not yet been developed.
27993213	8	94	theme	cortical	1550:1557	arg1	levels					1567:1572	lower cortical lactate levels	1544:1572	lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33)	1544:1622	Modified AL kidneys had significantly lower perfusate lactates (3.1 versus 4.1 mmol/L, P = 0.04) during reperfusion and lower cortical lactate levels (AL = 0.66 ± 0.31, UW = 0.89 ± 0.53 mM, P = 0.33).
27993213	10	95	theme	perfusate	1793:1801	arg1	levels					1811:1816	lower perfusate lactate levels	1787:1816	lower perfusate lactate levels	1787:1816	CONCLUSIONS We conclude that HMP with modified AL solution showed improved perfusion compared with UW and lower perfusate lactate levels during warm reperfusion.
27993213	3	96	theme	low	442:444	arg1	Ca2+					446:449	low Ca2+	442:449	low Ca2+	442:449	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	7	97	theme	glucose	1388:1394	arg1	consumption					1396:1406	glucose consumption	1388:1406	glucose consumption	1388:1406	During warm reperfusion, there were no significant differences between the two HMP groups in creatinine clearance, oxygen, and glucose consumption between groups.
27993213	3	98	theme	4	468:468	arg1	%					469:469	%	469:469	%	469:469	The aim of the present study was to examine a modified AL solution with low Ca2+, 16 mM Mg2+, and 4% albumin on kidney preservation compared with University Wisconsin solution (UW).
27993213	4	99	theme	reperfusion	699:709	arg1	2 h					679:681	2 h	679:681	2 h of normothermic reperfusion	679:709	METHODS Twenty donation of organs after cardiac death porcine kidneys underwent HMP for 10 h (AL, n = 10; UW, n = 10) and then 2 h of normothermic reperfusion.
27993213	0	100	theme	ex vivo	3:9	arg1	comparison					11:20	An ex vivo comparison	0:20	An ex vivo comparison of adenosine and lidocaine solution	0:56	An ex vivo comparison of adenosine and lidocaine solution and University of Wisconsin solution for hypothermic machine perfusion of porcine kidneys: potential for development.
27112885	4	0	theme	scanning	771:778	arg1	diffraction					953:963	X-ray diffraction	947:963	X-ray diffraction	947:963	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	0	theme	scanning	771:778	arg1	spectroscopy					859:870	energy dispersive X-ray spectroscopy	835:870	energy dispersive X-ray spectroscopy	835:870	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	0	theme	scanning	771:778	arg1	microscope					823:832	transmission electron microscope	801:832	transmission electron microscope	801:832	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	0	theme	scanning	771:778	arg1	spectroscopy					929:940	X-ray photoelectron spectroscopy	909:940	X-ray photoelectron spectroscopy	909:940	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	0	theme	scanning	771:778	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	0	theme	scanning	771:778	arg1	diffraction					896:906	area electron diffraction	882:906	area electron diffraction	882:906	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	2	1	theme	cellulose	388:396	arg1	hydrogels					398:406	cellulose hydrogels	388:406	cellulose hydrogels	388:406	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	5	2	theme	concentration	1274:1286	arg1	determination					1238:1250	determination	1238:1250	determination of minimal inhibitory concentration	1238:1286	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	5	2	theme	concentration	1274:1286	arg1	method					1227:1232	parallel streak method	1211:1232	parallel streak method	1211:1232	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	6	3	theme	materials	1448:1456	arg1	forms					1412:1416	novel forms	1406:1416	novel forms of cellulose-based functional materials	1406:1456	This work provides an example of combining cellulose aerogels with nanomaterials, and helps to develop novel forms of cellulose-based functional materials.
27112885	4	4	theme	electron	780:787	arg1	diffraction					953:963	X-ray diffraction	947:963	X-ray diffraction	947:963	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	4	theme	electron	780:787	arg1	spectroscopy					859:870	energy dispersive X-ray spectroscopy	835:870	energy dispersive X-ray spectroscopy	835:870	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	4	theme	electron	780:787	arg1	microscope					823:832	transmission electron microscope	801:832	transmission electron microscope	801:832	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	4	theme	electron	780:787	arg1	spectroscopy					929:940	X-ray photoelectron spectroscopy	909:940	X-ray photoelectron spectroscopy	909:940	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	4	theme	electron	780:787	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	4	theme	electron	780:787	arg1	diffraction					896:906	area electron diffraction	882:906	area electron diffraction	882:906	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	1	5	theme	various	293:299	arg1	nanomaterials					301:313	various nanomaterials	293:313	various nanomaterials	293:313	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	4	6	theme	electron	887:894	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	6	theme	electron	887:894	arg1	diffraction					896:906	area electron diffraction	882:906	area electron diffraction	882:906	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	5	7	theme	intracellular	1134:1146	arg1	monocytogenes					1119:1131	Listeria monocytogenes	1110:1131	Listeria monocytogenes (intracellular bacteria)	1110:1156	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	5	7	theme	intracellular	1134:1146	arg1	bacteria					1148:1155	intracellular bacteria	1134:1155	intracellular bacteria	1134:1155	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	3	8	theme	silver	593:598	arg1	nanoparticles					600:612	polypyrrole and silver nanoparticles	577:612	polypyrrole and silver nanoparticles	577:612	Prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ oxidative polymerization of pyrrole using silver ions as oxidizing agent.
27112885	3	9	theme	easily-operated	617:631	arg1	polymerization					651:664	easily-operated in-situ oxidative polymerization	617:664	easily-operated in-situ oxidative polymerization of pyrrole	617:675	Prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ oxidative polymerization of pyrrole using silver ions as oxidizing agent.
27112885	4	10	theme	energy	835:840	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	10	theme	energy	835:840	arg1	spectroscopy					859:870	energy dispersive X-ray spectroscopy	835:870	energy dispersive X-ray spectroscopy	835:870	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	2	11	used	used	471:474	arg2	solution					458:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	2	11	used	used	471:474	arg2	solvent					489:495	cellulose solvent	479:495	cellulose solvent	479:495	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	6	12	theme	cellulose-based	1421:1435	arg1	materials					1448:1456	cellulose-based functional materials	1421:1456	cellulose-based functional materials	1421:1456	This work provides an example of combining cellulose aerogels with nanomaterials, and helps to develop novel forms of cellulose-based functional materials.
27112885	5	13	theme	hybrid	1010:1015	arg1	aerogels					1017:1024	the hybrid aerogels	1006:1024	the hybrid aerogels	1006:1024	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	3	14	theme	polypyrrole	577:587	arg1	nanoparticles					600:612	polypyrrole and silver nanoparticles	577:612	polypyrrole and silver nanoparticles	577:612	Prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ oxidative polymerization of pyrrole using silver ions as oxidizing agent.
27112885	2	15	theme	inexpensive	421:431	arg1	solvent					489:495	cellulose solvent	479:495	cellulose solvent	479:495	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	2	15	theme	inexpensive	421:431	arg1	solution					458:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	0	16	theme	antibacterial	117:129	arg1	nanoparticles					62:74	polypyrrole and silver nanoparticles	39:74	polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity	39:138	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	0	16	theme	antibacterial	117:129	arg1	activity					131:138	antibacterial activity	117:138	antibacterial activity	117:138	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	5	17	theme	aerogels	1017:1024	arg1	activity					994:1001	the antibacterial activity	976:1001	the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria)	976:1156	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	4	18	theme	X-ray	947:951	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	18	theme	X-ray	947:951	arg1	diffraction					953:963	X-ray diffraction	947:963	X-ray diffraction	947:963	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	0	19	theme	Cellulose	0:8	arg1	aerogels					10:17	Cellulose aerogels	0:17	Cellulose aerogels	0:17	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	2	20	theme	green	415:419	arg1	solvent					489:495	cellulose solvent	479:495	cellulose solvent	479:495	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	2	20	theme	green	415:419	arg1	solution					458:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	5	21	theme	Listeria	1110:1117	arg1	monocytogenes					1119:1131	Listeria monocytogenes	1110:1131	Listeria monocytogenes (intracellular bacteria)	1110:1156	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	5	21	theme	Listeria	1110:1117	arg1	bacteria					1148:1155	intracellular bacteria	1134:1155	intracellular bacteria	1134:1155	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	5	22	theme	minimal	1255:1261	arg1	concentration					1274:1286	minimal inhibitory concentration	1255:1286	minimal inhibitory concentration	1255:1286	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	6	23	theme	novel	1406:1410	arg1	forms					1412:1416	novel forms	1406:1416	novel forms of cellulose-based functional materials	1406:1456	This work provides an example of combining cellulose aerogels with nanomaterials, and helps to develop novel forms of cellulose-based functional materials.
27112885	3	24	theme	in-situ	633:639	arg1	polymerization					651:664	easily-operated in-situ oxidative polymerization	617:664	easily-operated in-situ oxidative polymerization of pyrrole	617:675	Prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ oxidative polymerization of pyrrole using silver ions as oxidizing agent.
27112885	5	25	theme	antibacterial	980:992	arg1	activity					994:1001	the antibacterial activity	976:1001	the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria)	976:1156	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	2	26	theme	cellulose	479:487	arg1	solvent					489:495	cellulose solvent	479:495	cellulose solvent	479:495	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	2	26	theme	cellulose	479:487	arg1	solution					458:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	3	27	theme	oxidative	641:649	arg1	polymerization					651:664	easily-operated in-situ oxidative polymerization	617:664	easily-operated in-situ oxidative polymerization of pyrrole	617:675	Prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ oxidative polymerization of pyrrole using silver ions as oxidizing agent.
27112885	1	28	theme	promising	213:221	arg1	aerogels					180:187	Green porous and lightweight cellulose aerogels	141:187	Green porous and lightweight cellulose aerogels	141:187	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	1	28	theme	promising	213:221	arg1	candidates					223:232	promising candidates	213:232	promising candidates	213:232	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	4	29	theme	X-ray	909:913	arg1	spectroscopy					929:940	X-ray photoelectron spectroscopy	909:940	X-ray photoelectron spectroscopy	909:940	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	29	theme	X-ray	909:913	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	2	30	theme	wheat	336:340	arg1	feedstock					365:373	feedstock	365:373	feedstock	365:373	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	2	30	theme	wheat	336:340	arg1	straw					342:346	waste wheat straw	330:346	waste wheat straw	330:346	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	3	31	theme	pyrrole	669:675	arg1	polymerization					651:664	easily-operated in-situ oxidative polymerization	617:664	easily-operated in-situ oxidative polymerization of pyrrole	617:675	Prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ oxidative polymerization of pyrrole using silver ions as oxidizing agent.
27112885	4	32	theme	area	882:885	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	32	theme	area	882:885	arg1	diffraction					896:906	area electron diffraction	882:906	area electron diffraction	882:906	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	2	33	theme	waste	330:334	arg1	feedstock					365:373	feedstock	365:373	feedstock	365:373	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	2	33	theme	waste	330:334	arg1	straw					342:346	waste wheat straw	330:346	waste wheat straw	330:346	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	3	34	theme	freeze-drying	507:519	arg1	treatment					521:529	freeze-drying treatment	507:529	freeze-drying treatment	507:529	Prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ oxidative polymerization of pyrrole using silver ions as oxidizing agent.
27112885	3	35	theme	cellulose	536:544	arg1	hydrogels					546:554	the cellulose hydrogels	532:554	the cellulose hydrogels	532:554	Prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ oxidative polymerization of pyrrole using silver ions as oxidizing agent.
27112885	4	36	theme	tri-component	719:731	arg1	aerogels					740:747	The tri-component hybrid aerogels	715:747	The tri-component hybrid aerogels	715:747	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	0	37	dep	nanoparticles	62:74	arg1	synthesis					85:93	In-situ synthesis	77:93	In-situ synthesis	77:93	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	0	37	dep	nanoparticles	62:74	arg1	nanoparticles					62:74	polypyrrole and silver nanoparticles	39:74	polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity	39:138	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	0	37	dep	nanoparticles	62:74	arg1	characterization					96:111	characterization	96:111	characterization	96:111	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	0	37	dep	nanoparticles	62:74	arg1	activity					131:138	antibacterial activity	117:138	antibacterial activity	117:138	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	2	38	theme	glycol	451:456	arg1	solvent					489:495	cellulose solvent	479:495	cellulose solvent	479:495	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	2	38	theme	glycol	451:456	arg1	solution					458:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	0	39	theme	polypyrrole	39:49	arg1	synthesis					85:93	In-situ synthesis	77:93	In-situ synthesis	77:93	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	0	39	theme	polypyrrole	39:49	arg1	activity					131:138	antibacterial activity	117:138	antibacterial activity	117:138	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	0	39	theme	polypyrrole	39:49	arg1	characterization					96:111	characterization	96:111	characterization	96:111	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	0	39	theme	polypyrrole	39:49	arg1	nanoparticles					62:74	polypyrrole and silver nanoparticles	39:74	polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity	39:138	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	5	40	theme	streak	1220:1225	arg1	method					1227:1232	parallel streak method	1211:1232	parallel streak method	1211:1232	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	4	41	theme	photoelectron	915:927	arg1	spectroscopy					929:940	X-ray photoelectron spectroscopy	909:940	X-ray photoelectron spectroscopy	909:940	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	41	theme	photoelectron	915:927	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	2	42	theme	NaOH/polyethylene	433:449	arg1	solvent					489:495	cellulose solvent	479:495	cellulose solvent	479:495	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	2	42	theme	NaOH/polyethylene	433:449	arg1	solution					458:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	a green inexpensive NaOH/polyethylene glycol solution	413:465	In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent.
27112885	5	43	dep	coli	1046:1049	arg1	Gram-negative					1052:1064	Gram-negative	1052:1064	Gram-negative	1052:1064	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	4	44	theme	transmission	801:812	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	44	theme	transmission	801:812	arg1	microscope					823:832	transmission electron microscope	801:832	transmission electron microscope	801:832	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	0	45	theme	silver	55:60	arg1	synthesis					85:93	In-situ synthesis	77:93	In-situ synthesis	77:93	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	0	45	theme	silver	55:60	arg1	activity					131:138	antibacterial activity	117:138	antibacterial activity	117:138	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	0	45	theme	silver	55:60	arg1	characterization					96:111	characterization	96:111	characterization	96:111	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	0	45	theme	silver	55:60	arg1	nanoparticles					62:74	polypyrrole and silver nanoparticles	39:74	polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity	39:138	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	5	46	theme	parallel	1211:1218	arg1	method					1227:1232	parallel streak method	1211:1232	parallel streak method	1211:1232	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	1	47	theme	Green	141:145	arg1	aerogels					180:187	Green porous and lightweight cellulose aerogels	141:187	Green porous and lightweight cellulose aerogels	141:187	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	1	47	theme	Green	141:145	arg1	candidates					223:232	promising candidates	213:232	promising candidates	213:232	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	1	48	theme	porous	147:152	arg1	aerogels					180:187	Green porous and lightweight cellulose aerogels	141:187	Green porous and lightweight cellulose aerogels	141:187	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	1	48	theme	porous	147:152	arg1	candidates					223:232	promising candidates	213:232	promising candidates	213:232	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	4	49	theme	X-ray	853:857	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	49	theme	X-ray	853:857	arg1	spectroscopy					859:870	energy dispersive X-ray spectroscopy	835:870	energy dispersive X-ray spectroscopy	835:870	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	1	50	theme	petrochemical	253:265	arg1	materials					272:280	some petrochemical host materials	248:280	some petrochemical host materials	248:280	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	4	51	theme	selected	873:880	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	51	theme	selected	873:880	arg1	diffraction					896:906	area electron diffraction	882:906	area electron diffraction	882:906	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	3	52	theme	oxidizing	698:706	arg1	agent					708:712	oxidizing agent	698:712	oxidizing agent	698:712	Prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ oxidative polymerization of pyrrole using silver ions as oxidizing agent.
27112885	4	53	theme	dispersive	842:851	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	53	theme	dispersive	842:851	arg1	spectroscopy					859:870	energy dispersive X-ray spectroscopy	835:870	energy dispersive X-ray spectroscopy	835:870	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	54	theme	electron	814:821	arg1	microscope					789:798	scanning electron microscope	771:798	scanning electron microscope	771:798	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	4	54	theme	electron	814:821	arg1	microscope					823:832	transmission electron microscope	801:832	transmission electron microscope	801:832	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	1	55	theme	host	267:270	arg1	materials					272:280	some petrochemical host materials	248:280	some petrochemical host materials	248:280	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	0	56	theme	In-situ	77:83	arg1	synthesis					85:93	In-situ synthesis	77:93	In-situ synthesis	77:93	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	0	56	theme	In-situ	77:83	arg1	nanoparticles					62:74	polypyrrole and silver nanoparticles	39:74	polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity	39:138	Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.
27112885	3	57	theme	silver	683:688	arg1	ions					690:693	silver ions	683:693	silver ions	683:693	Prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ oxidative polymerization of pyrrole using silver ions as oxidizing agent.
27112885	1	58	theme	lightweight	158:168	arg1	aerogels					180:187	Green porous and lightweight cellulose aerogels	141:187	Green porous and lightweight cellulose aerogels	141:187	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	1	58	theme	lightweight	158:168	arg1	candidates					223:232	promising candidates	213:232	promising candidates	213:232	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	4	59	theme	hybrid	733:738	arg1	aerogels					740:747	The tri-component hybrid aerogels	715:747	The tri-component hybrid aerogels	715:747	The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction.
27112885	5	60	theme	inhibitory	1263:1272	arg1	concentration					1274:1286	minimal inhibitory concentration	1255:1286	minimal inhibitory concentration	1255:1286	Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively.
27112885	6	61	theme	cellulose	1346:1354	arg1	aerogels					1356:1363	cellulose aerogels	1346:1363	cellulose aerogels	1346:1363	This work provides an example of combining cellulose aerogels with nanomaterials, and helps to develop novel forms of cellulose-based functional materials.
27112885	1	62	theme	cellulose	170:178	arg1	aerogels					180:187	Green porous and lightweight cellulose aerogels	141:187	Green porous and lightweight cellulose aerogels	141:187	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	1	62	theme	cellulose	170:178	arg1	candidates					223:232	promising candidates	213:232	promising candidates	213:232	Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials.
27112885	6	63	theme	functional	1437:1446	arg1	materials					1448:1456	cellulose-based functional materials	1421:1456	cellulose-based functional materials	1421:1456	This work provides an example of combining cellulose aerogels with nanomaterials, and helps to develop novel forms of cellulose-based functional materials.
24945802	0	0	theme	human	70:74	arg1	fumigatus					97:105	the human pathogen Aspergillus fumigatus	66:105	the human pathogen Aspergillus fumigatus	66:105	Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
24945802	6	1	theme	in	1050:1051	arg1	virulence					1058:1066	in vivo virulence	1050:1066	in vivo virulence	1050:1066	Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.
24945802	5	2	theme	lung	814:817	arg1	burden					826:831	lung fungal burden	814:831	lung fungal burden	814:831	Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293.
24945802	0	3	from	growth	18:23	arg1	fumigatus					97:105	the human pathogen Aspergillus fumigatus	66:105	the human pathogen Aspergillus fumigatus	66:105	Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
24945802	2	4	theme	host-pathogen	387:399	arg1	studies					401:407	host-pathogen studies	387:407	host-pathogen studies of A. fumigatus-mediated disease	387:440	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	0	5	theme	Aspergillus	85:95	arg1	fumigatus					97:105	the human pathogen Aspergillus fumigatus	66:105	the human pathogen Aspergillus fumigatus	66:105	Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
24945802	3	6	theme	environmental	552:564	arg1	Af5517					591:596	Af5517	591:596	Af5517	591:596	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	3	6	theme	environmental	552:564	arg1	isolate					566:572	a single environmental isolate	543:572	a single environmental isolate of A. fumigatus	543:588	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	3	7	theme	fumigatus	580:588	arg1	Af5517					591:596	Af5517	591:596	Af5517	591:596	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	3	7	theme	fumigatus	580:588	arg1	isolate					566:572	a single environmental isolate	543:572	a single environmental isolate of A. fumigatus	543:588	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	0	8	theme	pathogen	76:83	arg1	fumigatus					97:105	the human pathogen Aspergillus fumigatus	66:105	the human pathogen Aspergillus fumigatus	66:105	Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
24945802	1	9	dep	pathogen	130:137	arg1	fumigatus					151:159	Aspergillus fumigatus	139:159	The ubiquitous fungal pathogen Aspergillus fumigatus	108:159	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	6	10	theme	growth	1033:1038	arg1	rates					1040:1044	in vitro growth rates	1024:1044	in vitro growth rates	1024:1044	Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.
24945802	4	11	theme	chitin	734:739	arg1	content					741:747	cell wall β-glucan and chitin content	711:747	content	741:747	Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased.
24945802	5	12	theme	neutropenic	873:883	arg1	mice					901:904	neutropenic Af5517-infected mice	873:904	neutropenic Af5517-infected mice	873:904	Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293.
24945802	2	13	from	considerations	369:382	arg1	studies					401:407	host-pathogen studies	387:407	host-pathogen studies of A. fumigatus-mediated disease	387:440	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	1	14	theme	allergic	178:185	arg1	sensitization					187:199	allergic sensitization	178:199	allergic sensitization	178:199	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	3	15	theme	decreased	455:463	arg1	growth					472:477	decreased radial growth	455:477	decreased radial growth	455:477	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	5	16	theme	fungal	819:824	arg1	burden					826:831	lung fungal burden	814:831	lung fungal burden	814:831	Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293.
24945802	3	17	theme	germination	488:498	arg1	ability					505:511	ability	505:511	ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates	505:656	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	3	17	theme	germination	488:498	arg1	rate					480:483	rate	480:483	rate of germination	480:498	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	3	17	theme	germination	488:498	arg1	growth					472:477	decreased radial growth	455:477	decreased radial growth	455:477	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	5	18	theme	tissue	838:843	arg1	pathology					845:853	tissue pathology	838:853	tissue pathology	838:853	Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293.
24945802	2	19	theme	disease	434:440	arg1	studies					401:407	host-pathogen studies	387:407	host-pathogen studies of A. fumigatus-mediated disease	387:440	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	0	20	theme	Isolate-dependent	0:16	arg1	growth					18:23	Isolate-dependent growth	0:23	Isolate-dependent growth	0:23	Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
24945802	6	21	from	changes	1089:1095	arg1	composition					1110:1120	cell wall composition	1100:1120	cell wall composition	1100:1120	Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.
24945802	2	22	theme	environmental	332:344	arg1	isolates					346:353	clinical and environmental isolates	319:353	clinical and environmental isolates	319:353	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	1	23	theme	sensitization	187:199	arg1	mediator					166:173	a mediator	164:173	a mediator of allergic sensitization and invasive disease in susceptible individuals	164:247	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	1	23	theme	sensitization	187:199	arg1	pathogen					130:137	The ubiquitous fungal pathogen	108:137	The ubiquitous fungal pathogen Aspergillus fumigatus	108:159	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	5	24	theme	Af5517-infected	885:899	arg1	mice					901:904	neutropenic Af5517-infected mice	873:904	neutropenic Af5517-infected mice	873:904	Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293.
24945802	3	25	theme	environmental	635:647	arg1	isolates					649:656	other clinical and environmental isolates	616:656	other clinical and environmental isolates	616:656	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	6	26	dep	in	1050:1051	arg1	vivo					1053:1056	vivo	1053:1056	vivo	1053:1056	Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.
24945802	2	27	theme	clinical	319:326	arg1	isolates					346:353	clinical and environmental isolates	319:353	clinical and environmental isolates	319:353	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	1	28	theme	invasive	205:212	arg1	disease					214:220	invasive disease	205:220	invasive disease	205:220	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	3	29	theme	radial	465:470	arg1	growth					472:477	decreased radial growth	455:477	decreased radial growth	455:477	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	4	30	theme	wall	716:719	arg1	β-glucan					721:728	cell wall β-glucan and chitin content	711:747	β-glucan	721:728	Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased.
24945802	4	31	theme	hyphal	691:696	arg1	diameter					698:705	increased hyphal diameter	681:705	increased hyphal diameter	681:705	Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased.
24945802	3	32	theme	clinical	622:629	arg1	isolates					649:656	other clinical and environmental isolates	616:656	other clinical and environmental isolates	616:656	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	2	33	theme	fumigatus-mediated	415:432	arg1	disease					434:440	A. fumigatus-mediated disease	412:440	A. fumigatus-mediated disease	412:440	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	1	34	theme	disease	214:220	arg1	mediator					166:173	a mediator	164:173	a mediator of allergic sensitization and invasive disease in susceptible individuals	164:247	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	1	34	theme	disease	214:220	arg1	pathogen					130:137	The ubiquitous fungal pathogen	108:137	The ubiquitous fungal pathogen Aspergillus fumigatus	108:159	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	6	35	theme	cell	1100:1103	arg1	composition					1110:1120	cell wall composition	1100:1120	cell wall composition	1100:1120	Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.
24945802	4	36	theme	increased	681:689	arg1	diameter					698:705	increased hyphal diameter	681:705	increased hyphal diameter	681:705	Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased.
24945802	0	37	theme	wall	46:49	arg1	composition					51:61	cell wall composition	41:61	cell wall composition	41:61	Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
24945802	5	38	theme	clinical	927:934	arg1	Af293					944:948	the clinical isolate Af293	923:948	the clinical isolate Af293	923:948	Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293.
24945802	0	39	from	composition	51:61	arg1	fumigatus					97:105	the human pathogen Aspergillus fumigatus	66:105	the human pathogen Aspergillus fumigatus	66:105	Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
24945802	0	40	theme	cell	41:44	arg1	composition					51:61	cell wall composition	41:61	cell wall composition	41:61	Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
24945802	6	41	theme	previous	971:978	arg1	findings					980:987	previous findings	971:987	previous findings that suggest a correlation between in vitro growth rates and in vivo virulence	971:1066	Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.
24945802	3	42	theme	other	616:620	arg1	isolates					649:656	other clinical and environmental isolates	616:656	other clinical and environmental isolates	616:656	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	3	43	theme	single	545:550	arg1	Af5517					591:596	Af5517	591:596	Af5517	591:596	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	3	43	theme	single	545:550	arg1	isolate					566:572	a single environmental isolate	543:572	a single environmental isolate of A. fumigatus	543:588	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	1	44	theme	ubiquitous	112:121	arg1	mediator					166:173	a mediator	164:173	a mediator of allergic sensitization and invasive disease in susceptible individuals	164:247	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	1	44	theme	ubiquitous	112:121	arg1	pathogen					130:137	The ubiquitous fungal pathogen	108:137	The ubiquitous fungal pathogen Aspergillus fumigatus	108:159	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	4	45	theme	cell	711:714	arg1	β-glucan					721:728	cell wall β-glucan and chitin content	711:747	β-glucan	721:728	Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased.
24945802	2	46	theme	phenotypic	278:287	arg1	considerations					369:382	important considerations	359:382	important considerations in host-pathogen studies of A. fumigatus-mediated disease	359:440	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	2	46	theme	phenotypic	278:287	arg1	variability					289:299	The significant genetic and phenotypic variability	250:299	The significant genetic and phenotypic variability between and among clinical and environmental isolates	250:353	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	1	47	theme	fungal	123:128	arg1	mediator					166:173	a mediator	164:173	a mediator of allergic sensitization and invasive disease in susceptible individuals	164:247	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	1	47	theme	fungal	123:128	arg1	pathogen					130:137	The ubiquitous fungal pathogen	108:137	The ubiquitous fungal pathogen Aspergillus fumigatus	108:159	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	1	48	theme	susceptible	225:235	arg1	individuals					237:247	susceptible individuals	225:247	susceptible individuals	225:247	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	3	49	theme	colony	526:531	arg1	growth					533:538	colony growth	526:538	colony growth	526:538	We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates.
24945802	6	50	theme	in	1024:1025	arg1	rates					1040:1044	in vitro growth rates	1024:1044	in vitro growth rates	1024:1044	Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.
24945802	2	51	theme	important	359:367	arg1	considerations					369:382	important considerations	359:382	important considerations in host-pathogen studies of A. fumigatus-mediated disease	359:440	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	2	51	theme	important	359:367	arg1	variability					289:299	The significant genetic and phenotypic variability	250:299	The significant genetic and phenotypic variability between and among clinical and environmental isolates	250:353	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	6	52	dep	in	1024:1025	arg1	vitro					1027:1031	vitro	1027:1031	vitro	1027:1031	Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.
24945802	2	53	theme	genetic	266:272	arg1	considerations					369:382	important considerations	359:382	important considerations in host-pathogen studies of A. fumigatus-mediated disease	359:440	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	2	53	theme	genetic	266:272	arg1	variability					289:299	The significant genetic and phenotypic variability	250:299	The significant genetic and phenotypic variability between and among clinical and environmental isolates	250:353	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	2	54	theme	A.	412:413	arg1	disease					434:440	A. fumigatus-mediated disease	412:440	A. fumigatus-mediated disease	412:440	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	6	55	theme	wall	1105:1108	arg1	composition					1110:1120	cell wall composition	1100:1120	cell wall composition	1100:1120	Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.
24945802	0	56	from	virulence	26:34	arg1	fumigatus					97:105	the human pathogen Aspergillus fumigatus	66:105	the human pathogen Aspergillus fumigatus	66:105	Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.
24945802	2	57	theme	significant	254:264	arg1	considerations					369:382	important considerations	359:382	important considerations in host-pathogen studies of A. fumigatus-mediated disease	359:440	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	2	57	theme	significant	254:264	arg1	variability					289:299	The significant genetic and phenotypic variability	250:299	The significant genetic and phenotypic variability between and among clinical and environmental isolates	250:353	The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease.
24945802	1	58	from	mediator	166:173	arg1	individuals					237:247	susceptible individuals	225:247	susceptible individuals	225:247	The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals.
24945802	5	59	theme	isolate	936:942	arg1	Af293					944:948	the clinical isolate Af293	923:948	the clinical isolate Af293	923:948	Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293.
25549904	4	0	theme	corn	913:916	arg1	residue					924:930	corn straw residue	913:930	corn straw residue	913:930	Analysis of lignocellulosic composition indicated that A. hydrogenigenes could increase removal rates of cellulose and hemicelluloses in corn straw residue by 12% and 5%, respectively.
25549904	1	1	theme	fermentation	188:199	arg1	bacterium					201:209	an acetate-type fermentation bacterium	172:209	an acetate-type fermentation bacterium in the phylum Bacteroidetes	172:237	The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw was evaluated by batch experiments.
25549904	5	2	theme	Further	961:967	arg1	experiment					969:978	Further experiment	961:978	Further experiment	961:978	Further experiment verified that the addition of A. hydrogenigenes could improve the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively) by 16.8% and 7.0%.
25549904	2	3	theme	growth	411:416	arg1	rate					418:421	relatively high growth rate	395:421	relatively high growth rate	395:421	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	5	4	dep	xylan	1085:1089	arg1	hemicelluloses					1117:1130	hemicelluloses	1117:1130	hemicelluloses	1117:1130	Further experiment verified that the addition of A. hydrogenigenes could improve the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively) by 16.8% and 7.0%.
25549904	5	4	dep	xylan	1085:1089	arg1	cellulose					1103:1111	models for cellulose	1092:1111	models for cellulose	1092:1111	Further experiment verified that the addition of A. hydrogenigenes could improve the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively) by 16.8% and 7.0%.
25549904	5	5	theme	methyl	1064:1069	arg1	cellulose					1071:1079	methyl cellulose	1064:1079	methyl cellulose	1064:1079	Further experiment verified that the addition of A. hydrogenigenes could improve the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively) by 16.8% and 7.0%.
25549904	3	6	theme	10	726:727	arg1	%					728:728	%	728:728	%	728:728	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	1	7	from	bacterium	201:209	arg1	phylum					218:223	the phylum	214:223	the phylum	214:223	The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw was evaluated by batch experiments.
25549904	5	8	theme	hydrogenigenes	1013:1026	arg1	addition					998:1005	the addition	994:1005	the addition of A. hydrogenigenes	994:1026	Further experiment verified that the addition of A. hydrogenigenes could improve the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively) by 16.8% and 7.0%.
25549904	1	9	theme	batch	297:301	arg1	experiments					303:313	batch experiments	297:313	batch experiments	297:313	The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw was evaluated by batch experiments.
25549904	0	10	theme	corn	125:128	arg1	straw					130:134	corn straw	125:134	corn straw	125:134	Bioaugmentation with an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes improves methane production from corn straw.
25549904	4	11	from	hemicelluloses	895:908	arg1	residue					924:930	corn straw residue	913:930	corn straw residue	913:930	Analysis of lignocellulosic composition indicated that A. hydrogenigenes could increase removal rates of cellulose and hemicelluloses in corn straw residue by 12% and 5%, respectively.
25549904	4	12	theme	straw	918:922	arg1	residue					924:930	corn straw residue	913:930	corn straw residue	913:930	Analysis of lignocellulosic composition indicated that A. hydrogenigenes could increase removal rates of cellulose and hemicelluloses in corn straw residue by 12% and 5%, respectively.
25549904	3	13	theme	209.3	752:756	arg1	control					743:749	control	743:749	control	743:749	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	3	13	theme	209.3	752:756	arg1	straw					768:772	209.3 mL/g-corn straw	752:772	209.3 mL/g-corn straw	752:772	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	1	14	with	bioaugmentation	151:165	arg1	bacterium					201:209	an acetate-type fermentation bacterium	172:209	an acetate-type fermentation bacterium in the phylum Bacteroidetes	172:237	The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw was evaluated by batch experiments.
25549904	2	15	theme	Acetobacteroides	316:331	arg1	hydrogenigenes					333:346	Acetobacteroides hydrogenigenes	316:346	Acetobacteroides hydrogenigenes	316:346	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	2	15	theme	Acetobacteroides	316:331	arg1	strain					363:368	a promising strain	351:368	a promising strain	351:368	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	3	16	with	bioaugmentation	592:606	arg1	hydrogenigenes					616:629	A. hydrogenigenes	613:629	A. hydrogenigenes	613:629	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	2	17	theme	polyoses	520:527	arg1	spectrum					486:493	a broad spectrum	478:493	a broad spectrum of pentoses, hexoses and polyoses	478:527	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	4	18	theme	lignocellulosic	788:802	arg1	composition					804:814	lignocellulosic composition	788:814	lignocellulosic composition	788:814	Analysis of lignocellulosic composition indicated that A. hydrogenigenes could increase removal rates of cellulose and hemicelluloses in corn straw residue by 12% and 5%, respectively.
25549904	2	19	theme	acetate	444:450	arg1	tolerance					452:460	acetate tolerance	444:460	acetate tolerance	444:460	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	2	20	theme	pentoses	498:505	arg1	spectrum					486:493	a broad spectrum	478:493	a broad spectrum of pentoses, hexoses and polyoses	478:527	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	5	21	theme	xylan	1085:1089	arg1	yields					1054:1059	the methane yields	1042:1059	the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively)	1042:1145	Further experiment verified that the addition of A. hydrogenigenes could improve the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively) by 16.8% and 7.0%.
25549904	1	22	from	effect	141:146	arg1	digestion					256:264	the anaerobic digestion	242:264	the anaerobic digestion of corn straw	242:278	The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw was evaluated by batch experiments.
25549904	4	23	theme	cellulose	881:889	arg1	hemicelluloses					895:908	hemicelluloses	895:908	hemicelluloses in corn straw residue	895:930	Analysis of lignocellulosic composition indicated that A. hydrogenigenes could increase removal rates of cellulose and hemicelluloses in corn straw residue by 12% and 5%, respectively.
25549904	4	23	theme	cellulose	881:889	arg1	rates					872:876	removal rates	864:876	removal rates of cellulose	864:889	Analysis of lignocellulosic composition indicated that A. hydrogenigenes could increase removal rates of cellulose and hemicelluloses in corn straw residue by 12% and 5%, respectively.
25549904	5	24	theme	models	1092:1097	arg1	cellulose					1103:1111	models for cellulose	1092:1111	models for cellulose	1092:1111	Further experiment verified that the addition of A. hydrogenigenes could improve the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively) by 16.8% and 7.0%.
25549904	0	25	theme	acetate-type	24:35	arg1	hydrogenigenes					77:90	an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes	21:90	an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes	21:90	Bioaugmentation with an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes improves methane production from corn straw.
25549904	4	26	theme	removal	864:870	arg1	rates					872:876	removal rates	864:876	removal rates of cellulose	864:889	Analysis of lignocellulosic composition indicated that A. hydrogenigenes could increase removal rates of cellulose and hemicelluloses in corn straw residue by 12% and 5%, respectively.
25549904	2	27	theme	promising	353:361	arg1	hydrogenigenes					333:346	Acetobacteroides hydrogenigenes	316:346	Acetobacteroides hydrogenigenes	316:346	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	2	27	theme	promising	353:361	arg1	strain					363:368	a promising strain	351:368	a promising strain	351:368	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	2	28	theme	broad	480:484	arg1	spectrum					486:493	a broad spectrum	478:493	a broad spectrum of pentoses, hexoses and polyoses	478:527	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	3	29	theme	mL/g-corn	758:766	arg1	control					743:749	control	743:749	control	743:749	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	3	29	theme	mL/g-corn	758:766	arg1	straw					768:772	209.3 mL/g-corn straw	752:772	209.3 mL/g-corn straw	752:772	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	3	30	theme	straw	575:579	arg1	digestion					581:589	corn straw digestion	570:589	corn straw digestion	570:589	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	0	31	theme	bacterium	50:58	arg1	hydrogenigenes					77:90	an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes	21:90	an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes	21:90	Bioaugmentation with an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes improves methane production from corn straw.
25549904	3	32	dep	inoculation	730:740	arg1	control					743:749	control	743:749	control	743:749	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	3	32	dep	inoculation	730:740	arg1	straw					768:772	209.3 mL/g-corn straw	752:772	209.3 mL/g-corn straw	752:772	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	2	33	with	bioaugmentation	374:388	arg1	rate					418:421	relatively high growth rate	395:421	relatively high growth rate	395:421	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	2	33	with	bioaugmentation	374:388	arg1	yields					433:438	hydrogen yields	424:438	hydrogen yields	424:438	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	2	33	with	bioaugmentation	374:388	arg1	tolerance					452:460	acetate tolerance	444:460	acetate tolerance	444:460	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	3	34	theme	%	643:643	arg1	increase					645:652	19-23% increase	638:652	19-23% increase of the methane yield	638:673	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	1	35	theme	anaerobic	246:254	arg1	digestion					256:264	the anaerobic digestion	242:264	the anaerobic digestion of corn straw	242:278	The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw was evaluated by batch experiments.
25549904	0	36	theme	fermentation	37:48	arg1	hydrogenigenes					77:90	an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes	21:90	an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes	21:90	Bioaugmentation with an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes improves methane production from corn straw.
25549904	5	37	theme	for	1099:1101	arg1	cellulose					1103:1111	models for cellulose	1092:1111	models for cellulose	1092:1111	Further experiment verified that the addition of A. hydrogenigenes could improve the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively) by 16.8% and 7.0%.
25549904	5	38	theme	methane	1046:1052	arg1	yields					1054:1059	the methane yields	1042:1059	the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively)	1042:1145	Further experiment verified that the addition of A. hydrogenigenes could improve the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively) by 16.8% and 7.0%.
25549904	4	39	from	rates	872:876	arg1	residue					924:930	corn straw residue	913:930	corn straw residue	913:930	Analysis of lignocellulosic composition indicated that A. hydrogenigenes could increase removal rates of cellulose and hemicelluloses in corn straw residue by 12% and 5%, respectively.
25549904	2	40	theme	hexoses	508:514	arg1	spectrum					486:493	a broad spectrum	478:493	a broad spectrum of pentoses, hexoses and polyoses	478:527	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	2	41	theme	high	406:409	arg1	rate					418:421	relatively high growth rate	395:421	relatively high growth rate	395:421	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	0	42	theme	Acetobacteroides	60:75	arg1	hydrogenigenes					77:90	an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes	21:90	an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes	21:90	Bioaugmentation with an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes improves methane production from corn straw.
25549904	3	43	theme	straw	708:712	arg1	maximum					681:687	maximum	681:687	maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw)	681:773	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	3	44	theme	19-23	638:642	arg1	%					643:643	%	643:643	%	643:643	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	5	45	theme	cellulose	1071:1079	arg1	yields					1054:1059	the methane yields	1042:1059	the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively)	1042:1145	Further experiment verified that the addition of A. hydrogenigenes could improve the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively) by 16.8% and 7.0%.
25549904	3	46	theme	methane	661:667	arg1	yield					669:673	the methane yield	657:673	the methane yield	657:673	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	0	47	with	Bioaugmentation	0:14	arg1	hydrogenigenes					77:90	an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes	21:90	an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes	21:90	Bioaugmentation with an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes improves methane production from corn straw.
25549904	1	48	theme	bioaugmentation	151:165	arg1	effect					141:146	The effect	137:146	The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw	137:278	The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw was evaluated by batch experiments.
25549904	4	49	theme	composition	804:814	arg1	Analysis					776:783	Analysis	776:783	Analysis of lignocellulosic composition	776:814	Analysis of lignocellulosic composition indicated that A. hydrogenigenes could increase removal rates of cellulose and hemicelluloses in corn straw residue by 12% and 5%, respectively.
25549904	0	50	from	straw	130:134	arg1	production					109:118	methane production	101:118	methane production from corn straw	101:134	Bioaugmentation with an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes improves methane production from corn straw.
25549904	3	51	theme	yield	669:673	arg1	increase					645:652	19-23% increase	638:652	19-23% increase of the methane yield	638:673	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	3	52	theme	corn	570:573	arg1	digestion					581:589	corn straw digestion	570:589	corn straw digestion	570:589	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	1	53	theme	corn	269:272	arg1	straw					274:278	corn straw	269:278	corn straw	269:278	The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw was evaluated by batch experiments.
25549904	0	54	theme	methane	101:107	arg1	production					109:118	methane production	101:118	methane production from corn straw	101:134	Bioaugmentation with an acetate-type fermentation bacterium Acetobacteroides hydrogenigenes improves methane production from corn straw.
25549904	3	55	theme	mL/g-corn	698:706	arg1	straw					708:712	258.1 mL/g-corn straw	692:712	258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw)	692:773	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	2	56	theme	hydrogen	424:431	arg1	yields					433:438	hydrogen yields	424:438	hydrogen yields	424:438	Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen.
25549904	1	57	theme	straw	274:278	arg1	digestion					256:264	the anaerobic digestion	242:264	the anaerobic digestion of corn straw	242:278	The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw was evaluated by batch experiments.
25549904	3	58	theme	%	728:728	arg1	inoculation					730:740	10% inoculation	726:740	10% inoculation (control, 209.3 mL/g-corn straw)	726:773	During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw).
25549904	1	59	theme	acetate-type	175:186	arg1	bacterium					201:209	an acetate-type fermentation bacterium	172:209	an acetate-type fermentation bacterium in the phylum Bacteroidetes	172:237	The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw was evaluated by batch experiments.
26176779	8	0	theme	inulin	1136:1141	arg1	pigs					1156:1159	inulin supplemented pigs	1136:1159	inulin supplemented pigs	1136:1159	was found significantly greater in inulin supplemented pigs.
26176779	10	1	theme	OTUs	1375:1378	arg1	abundance					1362:1370	the abundance	1358:1370	the abundance of OTUs representing pathogenic microorganisms	1358:1417	In contrast, the abundance of OTUs representing pathogenic microorganisms Atopostipes suicloacalis and Bartonella quintana str.
26176779	4	2	theme	significant	684:694	arg1	changes					696:702	significant changes	684:702	significant changes in the microbial abundance based on operational taxonomic units (OTUs)	684:773	The study revealed significant changes in the microbial abundance based on operational taxonomic units (OTUs) among the groups.
26176779	2	3	theme	microflora	439:448	arg1	composition					450:460	fecal microflora composition	433:460	fecal microflora composition	433:460	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	9	4	theme	ginger	1319:1324	arg1	pigs					1339:1342	ginger supplemented pigs	1319:1342	ginger supplemented pigs	1319:1342	Similarly, the abundance of OTUs representing Bacteroides intestinalis, Selonomonas sp., and Phascolarcobacterium faecium was found significantly greater in ginger supplemented pigs.
26176779	12	5	dep	phylotypes	1600:1609	arg1	control-tetracycline					1630:1649	control-tetracycline	1630:1649	control-tetracycline	1630:1649	The OTUs were found to be clustered under two major phylotypes; ginger-inulin and control-tetracycline.
26176779	12	5	dep	phylotypes	1600:1609	arg1	ginger-inulin					1612:1624	ginger-inulin	1612:1624	ginger-inulin	1612:1624	The OTUs were found to be clustered under two major phylotypes; ginger-inulin and control-tetracycline.
26176779	12	5	dep	phylotypes	1600:1609	arg1	phylotypes					1600:1609	two major phylotypes	1590:1609	two major phylotypes; ginger-inulin and control-tetracycline	1590:1649	The OTUs were found to be clustered under two major phylotypes; ginger-inulin and control-tetracycline.
26176779	2	6	theme	Zingiber	321:328	arg1	ginger					313:318	ginger	313:318	ginger	313:318	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	2	6	theme	Zingiber	321:328	arg1	officinale					330:339	Zingiber officinale	321:339	Zingiber officinale	321:339	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	10	7	dep	Bartonella	1448:1457	arg1	str					1468:1470	str	1468:1470	Bartonella quintana str	1448:1470	In contrast, the abundance of OTUs representing pathogenic microorganisms Atopostipes suicloacalis and Bartonella quintana str.
26176779	10	7	dep	Bartonella	1448:1457	arg1	quintana					1459:1466	Bartonella quintana str	1448:1470	Bartonella quintana str	1448:1470	In contrast, the abundance of OTUs representing pathogenic microorganisms Atopostipes suicloacalis and Bartonella quintana str.
26176779	5	8	theme	OTUs	881:884	arg1	length					871:876	the fragment length	858:876	the fragment length of OTUs generated with three restriction enzymes (MspI, Sau3AI and BsuRI)	858:950	Presumptive identification of organisms was established based on the fragment length of OTUs generated with three restriction enzymes (MspI, Sau3AI and BsuRI).
26176779	0	9	from	Assessment	0:9	arg1	Pigs					42:45	Pigs	42:45	Pigs	42:45	Assessment of Fecal Microflora Changes in Pigs Supplemented with Herbal Residue and Prebiotic.
26176779	10	10	theme	pathogenic	1393:1402	arg1	microorganisms					1404:1417	pathogenic microorganisms	1393:1417	pathogenic microorganisms	1393:1417	In contrast, the abundance of OTUs representing pathogenic microorganisms Atopostipes suicloacalis and Bartonella quintana str.
26176779	3	11	theme	grower	523:528	arg1	pigs					530:533	The grower pigs	519:533	The grower pigs	519:533	The grower pigs were offered feed containing antibiotic (tetracycline), ginger and inulin separately and un-supplemented group served as control.
26176779	9	12	located	found	1288:1292	arg2	abundance					1177:1185	the abundance	1173:1185	the abundance of OTUs representing Bacteroides intestinalis, Selonomonas sp., and Phascolarcobacterium faecium	1173:1282	Similarly, the abundance of OTUs representing Bacteroides intestinalis, Selonomonas sp., and Phascolarcobacterium faecium was found significantly greater in ginger supplemented pigs.
26176779	9	12	located	found	1288:1292	arg1	pigs					1339:1342	ginger supplemented pigs	1319:1342	ginger supplemented pigs	1319:1342	Similarly, the abundance of OTUs representing Bacteroides intestinalis, Selonomonas sp., and Phascolarcobacterium faecium was found significantly greater in ginger supplemented pigs.
26176779	12	13	theme	major	1594:1598	arg1	control-tetracycline					1630:1649	control-tetracycline	1630:1649	control-tetracycline	1630:1649	The OTUs were found to be clustered under two major phylotypes; ginger-inulin and control-tetracycline.
26176779	12	13	theme	major	1594:1598	arg1	ginger-inulin					1612:1624	ginger-inulin	1612:1624	ginger-inulin	1612:1624	The OTUs were found to be clustered under two major phylotypes; ginger-inulin and control-tetracycline.
26176779	12	13	theme	major	1594:1598	arg1	phylotypes					1600:1609	two major phylotypes	1590:1609	two major phylotypes; ginger-inulin and control-tetracycline	1590:1649	The OTUs were found to be clustered under two major phylotypes; ginger-inulin and control-tetracycline.
26176779	2	14	theme	length	498:503	arg1	polymorphism					505:516	terminal restriction fragment length polymorphism	468:516	terminal restriction fragment length polymorphism	468:516	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	9	15	theme	OTUs	1190:1193	arg1	abundance					1177:1185	the abundance	1173:1185	the abundance of OTUs representing Bacteroides intestinalis, Selonomonas sp., and Phascolarcobacterium faecium	1173:1282	Similarly, the abundance of OTUs representing Bacteroides intestinalis, Selonomonas sp., and Phascolarcobacterium faecium was found significantly greater in ginger supplemented pigs.
26176779	4	16	theme	taxonomic	752:760	arg1	units					762:766	operational taxonomic units	740:766	operational taxonomic units (OTUs)	740:773	The study revealed significant changes in the microbial abundance based on operational taxonomic units (OTUs) among the groups.
26176779	4	16	theme	taxonomic	752:760	arg1	OTUs					769:772	OTUs	769:772	OTUs	769:772	The study revealed significant changes in the microbial abundance based on operational taxonomic units (OTUs) among the groups.
26176779	2	17	theme	fragment	489:496	arg1	polymorphism					505:516	terminal restriction fragment length polymorphism	468:516	terminal restriction fragment length polymorphism	468:516	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	0	18	from	Pigs	42:45	arg1	Assessment					0:9	Assessment	0:9	Assessment of Fecal Microflora Changes in Pigs	0:45	Assessment of Fecal Microflora Changes in Pigs Supplemented with Herbal Residue and Prebiotic.
26176779	5	19	theme	fragment	862:869	arg1	length					871:876	the fragment length	858:876	the fragment length of OTUs generated with three restriction enzymes (MspI, Sau3AI and BsuRI)	858:950	Presumptive identification of organisms was established based on the fragment length of OTUs generated with three restriction enzymes (MspI, Sau3AI and BsuRI).
26176779	2	20	theme	restriction	477:487	arg1	polymorphism					505:516	terminal restriction fragment length polymorphism	468:516	terminal restriction fragment length polymorphism	468:516	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	14	21	theme	ginger	1777:1782	arg1	potential					1764:1772	the potential	1760:1772	the potential of ginger and prebioticsto replace antibiotics in the diet of grower pig	1760:1845	The results suggest the potential of ginger and prebioticsto replace antibiotics in the diet of grower pig.
26176779	2	22	theme	present	248:254	arg1	study					256:260	The present study	244:260	The present study	244:260	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	1	23	theme	public	161:166	arg1	issue					175:179	public health issue	161:179	public health issue due to its negative impact on consumer health and environment	161:241	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	1	23	theme	public	161:166	arg1	usage					106:110	Antibiotic usage	95:110	Antibiotic usage in animals as a growth promoter	95:142	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	0	24	from	Changes	31:37	arg1	Pigs					42:45	Pigs	42:45	Pigs	42:45	Assessment of Fecal Microflora Changes in Pigs Supplemented with Herbal Residue and Prebiotic.
26176779	2	25	theme	terminal	468:475	arg1	polymorphism					505:516	terminal restriction fragment length polymorphism	468:516	terminal restriction fragment length polymorphism	468:516	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	2	26	theme	herbal	297:302	arg1	residue					304:310	herbal residue	297:310	herbal residue (ginger, Zingiber officinale, dried rhizome powder)	297:362	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	6	27	theme	Methylobacterium	1058:1073	arg1	intestinalis					1000:1011	Bacteroides intestinalis	988:1011	Bacteroides intestinalis	988:1011	The abundance of OTUs representing Bacteroides intestinalis, Eubacterium oxidoreducens, Selonomonas sp., Methylobacterium sp.
26176779	6	27	theme	Methylobacterium	1058:1073	arg1	sp					1075:1076	Methylobacterium sp	1058:1076	Methylobacterium sp	1058:1076	The abundance of OTUs representing Bacteroides intestinalis, Eubacterium oxidoreducens, Selonomonas sp., Methylobacterium sp.
26176779	1	28	theme	health	168:173	arg1	issue					175:179	public health issue	161:179	public health issue due to its negative impact on consumer health and environment	161:241	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	1	28	theme	health	168:173	arg1	usage					106:110	Antibiotic usage	95:110	Antibiotic usage in animals as a growth promoter	95:142	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	5	29	theme	Presumptive	793:803	arg1	identification					805:818	Presumptive identification	793:818	Presumptive identification of organisms	793:831	Presumptive identification of organisms was established based on the fragment length of OTUs generated with three restriction enzymes (MspI, Sau3AI and BsuRI).
26176779	7	30	theme	Denitrobacter	1083:1095	arg1	sp					1097:1098	Denitrobacter sp	1083:1098	Denitrobacter sp	1083:1098	and Denitrobacter sp.
26176779	2	31	dep	residue	304:310	arg1	ginger					313:318	ginger	313:318	ginger	313:318	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	2	31	dep	residue	304:310	arg1	officinale					330:339	Zingiber officinale	321:339	Zingiber officinale	321:339	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	2	31	dep	residue	304:310	arg1	powder					356:361	dried rhizome powder	342:361	dried rhizome powder	342:361	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	11	32	theme	inulin	1522:1527	arg1	pigs					1542:1545	ginger and inulin supplemented pigs	1511:1545	ginger and inulin supplemented pigs	1511:1545	Toulouse was significantly reduced in ginger and inulin supplemented pigs.
26176779	2	33	theme	rhizome	348:354	arg1	ginger					313:318	ginger	313:318	ginger	313:318	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	2	33	theme	rhizome	348:354	arg1	powder					356:361	dried rhizome powder	342:361	dried rhizome powder	342:361	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	0	34	theme	Microflora	20:29	arg1	Changes					31:37	Fecal Microflora Changes	14:37	Fecal Microflora Changes in Pigs	14:45	Assessment of Fecal Microflora Changes in Pigs Supplemented with Herbal Residue and Prebiotic.
26176779	1	35	theme	Antibiotic	95:104	arg1	issue					175:179	public health issue	161:179	public health issue due to its negative impact on consumer health and environment	161:241	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	1	35	theme	Antibiotic	95:104	arg1	usage					106:110	Antibiotic usage	95:110	Antibiotic usage in animals as a growth promoter	95:142	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	11	36	theme	supplemented	1529:1540	arg1	pigs					1542:1545	ginger and inulin supplemented pigs	1511:1545	ginger and inulin supplemented pigs	1511:1545	Toulouse was significantly reduced in ginger and inulin supplemented pigs.
26176779	1	37	theme	due	181:183	arg1	issue					175:179	public health issue	161:179	public health issue due to its negative impact on consumer health and environment	161:241	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	1	37	theme	due	181:183	arg1	usage					106:110	Antibiotic usage	95:110	Antibiotic usage in animals as a growth promoter	95:142	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	0	38	theme	Fecal	14:18	arg1	Changes					31:37	Fecal Microflora Changes	14:37	Fecal Microflora Changes in Pigs	14:45	Assessment of Fecal Microflora Changes in Pigs Supplemented with Herbal Residue and Prebiotic.
26176779	13	39	theme	inulin	1714:1719	arg1	pigs					1734:1737	ginger and inulin supplemented pigs	1703:1737	ginger and inulin supplemented pigs	1703:1737	Additionally, the abundance of OTUs was similar in ginger and inulin supplemented pigs.
26176779	3	40	theme	un-supplemented	624:638	arg1	group					640:644	un-supplemented group	624:644	un-supplemented group	624:644	The grower pigs were offered feed containing antibiotic (tetracycline), ginger and inulin separately and un-supplemented group served as control.
26176779	4	41	theme	operational	740:750	arg1	units					762:766	operational taxonomic units	740:766	operational taxonomic units (OTUs)	740:773	The study revealed significant changes in the microbial abundance based on operational taxonomic units (OTUs) among the groups.
26176779	4	41	theme	operational	740:750	arg1	OTUs					769:772	OTUs	769:772	OTUs	769:772	The study revealed significant changes in the microbial abundance based on operational taxonomic units (OTUs) among the groups.
26176779	11	42	theme	ginger	1511:1516	arg1	pigs					1542:1545	ginger and inulin supplemented pigs	1511:1545	ginger and inulin supplemented pigs	1511:1545	Toulouse was significantly reduced in ginger and inulin supplemented pigs.
26176779	2	43	theme	dried	342:346	arg1	ginger					313:318	ginger	313:318	ginger	313:318	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	2	43	theme	dried	342:346	arg1	powder					356:361	dried rhizome powder	342:361	dried rhizome powder	342:361	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	2	44	theme	fecal	433:437	arg1	composition					450:460	fecal microflora composition	433:460	fecal microflora composition	433:460	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	13	45	theme	supplemented	1721:1732	arg1	pigs					1734:1737	ginger and inulin supplemented pigs	1703:1737	ginger and inulin supplemented pigs	1703:1737	Additionally, the abundance of OTUs was similar in ginger and inulin supplemented pigs.
26176779	13	46	theme	OTUs	1683:1686	arg1	abundance					1670:1678	the abundance	1666:1678	the abundance of OTUs	1666:1686	Additionally, the abundance of OTUs was similar in ginger and inulin supplemented pigs.
26176779	13	46	theme	OTUs	1683:1686	arg1	similar					1692:1698	similar	1692:1698	similar	1692:1698	Additionally, the abundance of OTUs was similar in ginger and inulin supplemented pigs.
26176779	0	47	theme	Changes	31:37	arg1	Assessment					0:9	Assessment	0:9	Assessment of Fecal Microflora Changes in Pigs	0:45	Assessment of Fecal Microflora Changes in Pigs Supplemented with Herbal Residue and Prebiotic.
26176779	6	48	theme	OTUs	970:973	arg1	abundance					957:965	The abundance	953:965	The abundance of OTUs	953:973	The abundance of OTUs representing Bacteroides intestinalis, Eubacterium oxidoreducens, Selonomonas sp., Methylobacterium sp.
26176779	8	49	theme	supplemented	1143:1154	arg1	pigs					1156:1159	inulin supplemented pigs	1136:1159	inulin supplemented pigs	1136:1159	was found significantly greater in inulin supplemented pigs.
26176779	13	50	from	pigs	1734:1737	arg1	abundance					1670:1678	the abundance	1666:1678	the abundance of OTUs	1666:1686	Additionally, the abundance of OTUs was similar in ginger and inulin supplemented pigs.
26176779	13	50	from	pigs	1734:1737	arg1	similar					1692:1698	similar	1692:1698	similar	1692:1698	Additionally, the abundance of OTUs was similar in ginger and inulin supplemented pigs.
26176779	1	51	theme	negative	192:199	arg1	impact					201:206	its negative impact	188:206	its negative impact on consumer health and environment	188:241	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	9	52	theme	supplemented	1326:1337	arg1	pigs					1339:1342	ginger supplemented pigs	1319:1342	ginger supplemented pigs	1319:1342	Similarly, the abundance of OTUs representing Bacteroides intestinalis, Selonomonas sp., and Phascolarcobacterium faecium was found significantly greater in ginger supplemented pigs.
26176779	14	53	theme	pig	1843:1845	arg1	diet					1828:1831	the diet	1824:1831	the diet of grower pig	1824:1845	The results suggest the potential of ginger and prebioticsto replace antibiotics in the diet of grower pig.
26176779	14	54	dep	potential	1764:1772	arg1	replace					1801:1807	replace	1801:1807	replace antibiotics in the diet of grower pig	1801:1845	The results suggest the potential of ginger and prebioticsto replace antibiotics in the diet of grower pig.
26176779	5	55	theme	restriction	907:917	arg1	enzymes					919:925	three restriction enzymes	901:925	three restriction enzymes (MspI, Sau3AI and BsuRI)	901:950	Presumptive identification of organisms was established based on the fragment length of OTUs generated with three restriction enzymes (MspI, Sau3AI and BsuRI).
26176779	14	56	theme	grower	1836:1841	arg1	pig					1843:1845	grower pig	1836:1845	grower pig	1836:1845	The results suggest the potential of ginger and prebioticsto replace antibiotics in the diet of grower pig.
26176779	1	57	from	impact	201:206	arg1	environment					231:241	environment	231:241	environment	231:241	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	1	57	from	impact	201:206	arg1	health					220:225	consumer health	211:225	consumer health	211:225	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	4	58	theme	microbial	711:719	arg1	abundance					721:729	the microbial abundance	707:729	the microbial abundance based on operational taxonomic units (OTUs)	707:773	The study revealed significant changes in the microbial abundance based on operational taxonomic units (OTUs) among the groups.
26176779	1	59	from	usage	106:110	arg1	animals					115:121	animals	115:121	animals as a growth promoter	115:142	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	13	60	theme	ginger	1703:1708	arg1	pigs					1734:1737	ginger and inulin supplemented pigs	1703:1737	ginger and inulin supplemented pigs	1703:1737	Additionally, the abundance of OTUs was similar in ginger and inulin supplemented pigs.
26176779	5	61	dep	enzymes	919:925	arg1	Sau3AI					934:939	Sau3AI	934:939	Sau3AI	934:939	Presumptive identification of organisms was established based on the fragment length of OTUs generated with three restriction enzymes (MspI, Sau3AI and BsuRI).
26176779	5	61	dep	enzymes	919:925	arg1	MspI					928:931	MspI	928:931	MspI	928:931	Presumptive identification of organisms was established based on the fragment length of OTUs generated with three restriction enzymes (MspI, Sau3AI and BsuRI).
26176779	5	61	dep	enzymes	919:925	arg1	BsuRI					945:949	BsuRI	945:949	BsuRI	945:949	Presumptive identification of organisms was established based on the fragment length of OTUs generated with three restriction enzymes (MspI, Sau3AI and BsuRI).
26176779	2	62	theme	residue	304:310	arg1	alternative					393:403	an alternative	390:403	an alternative to antibiotics	390:418	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	2	62	theme	residue	304:310	arg1	effectiveness					280:292	effectiveness	280:292	effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin)	280:385	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	0	63	theme	Herbal	65:70	arg1	Residue					72:78	Herbal Residue	65:78	Herbal Residue	65:78	Assessment of Fecal Microflora Changes in Pigs Supplemented with Herbal Residue and Prebiotic.
26176779	2	64	theme	prebiotic	368:376	arg1	alternative					393:403	an alternative	390:403	an alternative to antibiotics	390:418	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	2	64	theme	prebiotic	368:376	arg1	effectiveness					280:292	effectiveness	280:292	effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin)	280:385	The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder) and prebiotic (inulin) as an alternative to antibiotics by comparing fecal microflora composition using terminal restriction fragment length polymorphism.
26176779	5	65	theme	organisms	823:831	arg1	identification					805:818	Presumptive identification	793:818	Presumptive identification of organisms	793:831	Presumptive identification of organisms was established based on the fragment length of OTUs generated with three restriction enzymes (MspI, Sau3AI and BsuRI).
26176779	1	66	theme	growth	128:133	arg1	promoter					135:142	a growth promoter	126:142	a growth promoter	126:142	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	4	67	from	changes	696:702	arg1	abundance					721:729	the microbial abundance	707:729	the microbial abundance based on operational taxonomic units (OTUs)	707:773	The study revealed significant changes in the microbial abundance based on operational taxonomic units (OTUs) among the groups.
26176779	1	68	theme	consumer	211:218	arg1	health					220:225	consumer health	211:225	consumer health	211:225	Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment.
26176779	14	69	theme	prebioticsto	1788:1799	arg1	potential					1764:1772	the potential	1760:1772	the potential of ginger and prebioticsto replace antibiotics in the diet of grower pig	1760:1845	The results suggest the potential of ginger and prebioticsto replace antibiotics in the diet of grower pig.
26176779	3	70	dep	offered	540:546	arg1	feed					548:551	feed	548:551	were offered feed containing antibiotic (tetracycline), ginger and inulin separately	535:618	The grower pigs were offered feed containing antibiotic (tetracycline), ginger and inulin separately and un-supplemented group served as control.
26176779	13	71	from	similar	1692:1698	arg1	pigs					1734:1737	ginger and inulin supplemented pigs	1703:1737	ginger and inulin supplemented pigs	1703:1737	Additionally, the abundance of OTUs was similar in ginger and inulin supplemented pigs.
29052468	0	0	theme	chewing	67:73	arg1	gums					75:78	chewing gums	67:78	chewing gums	67:78	Evaluation of the content of TiO2 nanoparticles in the coatings of chewing gums.
29052468	8	1	from	matrix	1287:1292	arg1	embedded					1264:1271	embedded	1264:1271	embedded	1264:1271	TiO2 particles constitute the entire coating of some chewing gums, whereas for others, TiO2 particles are embedded in an organic matrix and/or mixed with minerals like calcium carbonate, talc, or magnesium silicate.
29052468	11	2	theme	chewing	1672:1678	arg1	gum					1680:1682	chewing gum	1672:1682	chewing gum	1672:1682	The intake of nanoparticles is thus highly dependent on the kind of chewing gum, with an estimated range extending from 0.04 ± 0.01 to 0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum.
29052468	4	3	theme	TiO2	586:589	arg1	intake					576:581	the intake	572:581	the intake of TiO2	572:589	In this study, we focused our efforts on chewing gum, which is one of the food products contributing most to the intake of TiO2.
29052468	8	4	theme	organic	1279:1285	arg1	matrix					1287:1292	an organic matrix	1276:1292	an organic matrix	1276:1292	TiO2 particles constitute the entire coating of some chewing gums, whereas for others, TiO2 particles are embedded in an organic matrix and/or mixed with minerals like calcium carbonate, talc, or magnesium silicate.
29052468	6	5	theme	electron	776:783	arg1	TEM					797:799	TEM	797:799	TEM	797:799	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	6	5	theme	electron	776:783	arg1	microscopy					785:794	transmission electron microscopy	763:794	transmission electron microscopy (TEM)	763:800	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	1	6	theme	white	125:129	arg1	pigment					131:137	a white pigment	123:137	a white pigment in many food categories, including confectionery	123:186	Titanium dioxide is a metal oxide used as a white pigment in many food categories, including confectionery.
29052468	10	7	theme	particles	1534:1542	arg1	%					1525:1525	19 ± 4%	1519:1525	19 ± 4% of all particles	1519:1542	TiO2 nanoparticles account for 19 ± 4% of all particles, which represents a mass fraction of 4.2 ± 0.1% on average.
29052468	10	7	theme	particles	1534:1542	arg1	particles					1534:1542	all particles	1530:1542	all particles	1530:1542	TiO2 nanoparticles account for 19 ± 4% of all particles, which represents a mass fraction of 4.2 ± 0.1% on average.
29052468	6	8	theme	transmission	763:774	arg1	TEM					797:799	TEM	797:799	TEM	797:799	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	6	8	theme	transmission	763:774	arg1	microscopy					785:794	transmission electron microscopy	763:794	transmission electron microscopy (TEM)	763:800	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	9	9	theme	135 ± 42 nm	1475:1485	arg1	diameter					1463:1470	a mean diameter	1456:1470	a mean diameter of 135 ± 42 nm	1456:1485	We found 1.1 ± 0.3 to 17.3 ± 0.9 mg TiO2 particles per piece of chewing gum, with a mean diameter of 135 ± 42 nm.
29052468	9	10	theme	chewing	1438:1444	arg1	gum					1446:1448	chewing gum	1438:1448	chewing gum	1438:1448	We found 1.1 ± 0.3 to 17.3 ± 0.9 mg TiO2 particles per piece of chewing gum, with a mean diameter of 135 ± 42 nm.
29052468	0	11	theme	gums	75:78	arg1	coatings					55:62	the coatings	51:62	the coatings of chewing gums	51:78	Evaluation of the content of TiO2 nanoparticles in the coatings of chewing gums.
29052468	8	12	theme	magnesium	1354:1362	arg1	silicate					1364:1371	magnesium silicate	1354:1371	magnesium silicate	1354:1371	TiO2 particles constitute the entire coating of some chewing gums, whereas for others, TiO2 particles are embedded in an organic matrix and/or mixed with minerals like calcium carbonate, talc, or magnesium silicate.
29052468	3	13	from	products	443:450	arg1	present					427:433	present	427:433	present	427:433	To resolve this problem, a better estimation of the mass of nanoparticles present in food products is needed.
29052468	8	14	theme	entire	1188:1193	arg1	coating					1195:1201	the entire coating	1184:1201	the entire coating of some chewing gums	1184:1222	TiO2 particles constitute the entire coating of some chewing gums, whereas for others, TiO2 particles are embedded in an organic matrix and/or mixed with minerals like calcium carbonate, talc, or magnesium silicate.
29052468	6	15	theme	Raman	840:844	arg1	spectroscopy					846:857	Raman spectroscopy	840:857	Raman spectroscopy	840:857	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	2	16	theme	nanoparticles	295:307	arg1	intakes					279:285	the estimated intakes	265:285	the estimated intakes of TiO2 nanoparticles	265:307	Due to differences in the mass fraction of nanoparticles contained in TiO2, the estimated intakes of TiO2 nanoparticles differ by a factor of 10 in the literature.
29052468	11	17	with	dependent	1647:1655	arg1	range					1703:1707	an estimated range	1690:1707	an estimated range extending from 0.04 ± 0.01 to 0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum	1690:1790	The intake of nanoparticles is thus highly dependent on the kind of chewing gum, with an estimated range extending from 0.04 ± 0.01 to 0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum.
29052468	2	18	theme	nanoparticles	232:244	arg1	fraction					220:227	the mass fraction	211:227	the mass fraction of nanoparticles contained in TiO2	211:262	Due to differences in the mass fraction of nanoparticles contained in TiO2, the estimated intakes of TiO2 nanoparticles differ by a factor of 10 in the literature.
29052468	2	19	theme	10	331:332	arg1	factor					321:326	a factor	319:326	a factor of 10	319:332	Due to differences in the mass fraction of nanoparticles contained in TiO2, the estimated intakes of TiO2 nanoparticles differ by a factor of 10 in the literature.
29052468	5	20	theme	aqueous	717:723	arg1	phases					725:730	aqueous phases	717:730	aqueous phases	717:730	The coatings of four kinds of chewing gum, where the presence of TiO2 was confirmed by Raman spectroscopy, were extracted in aqueous phases.
29052468	5	21	theme	chewing	622:628	arg1	gum					630:632	chewing gum	622:632	chewing gum	622:632	The coatings of four kinds of chewing gum, where the presence of TiO2 was confirmed by Raman spectroscopy, were extracted in aqueous phases.
29052468	6	22	theme	size	989:992	arg1	distribution					994:1005	size distribution	989:1005	size distribution	989:1005	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	6	23	theme	plasma	884:889	arg1	ICP-AES					921:927	ICP-AES	921:927	ICP-AES	921:927	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	6	23	theme	plasma	884:889	arg1	spectroscopy					907:918	inductively coupled plasma atomic emission spectroscopy	864:918	inductively coupled plasma atomic emission spectroscopy (ICP-AES)	864:928	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	2	24	from	differences	196:206	arg1	fraction					220:227	the mass fraction	211:227	the mass fraction of nanoparticles contained in TiO2	211:262	Due to differences in the mass fraction of nanoparticles contained in TiO2, the estimated intakes of TiO2 nanoparticles differ by a factor of 10 in the literature.
29052468	4	25	theme	products	542:549	arg1	one					526:528	one	526:528	one	526:528	In this study, we focused our efforts on chewing gum, which is one of the food products contributing most to the intake of TiO2.
29052468	4	25	theme	products	542:549	arg1	products					542:549	the food products	533:549	the food products contributing most to the intake of TiO2	533:589	In this study, we focused our efforts on chewing gum, which is one of the food products contributing most to the intake of TiO2.
29052468	7	26	theme	gums	1041:1044	arg1	coatings					1012:1019	The coatings	1008:1019	The coatings of the four chewing gums	1008:1044	The coatings of the four chewing gums differ chemically from each other, and more specifically the amount of TiO2 varies from one coating to another.
29052468	10	27	theme	mass	1564:1567	arg1	fraction					1569:1576	a mass fraction	1562:1576	a mass fraction of 4.2 ± 0.1% on average	1562:1601	TiO2 nanoparticles account for 19 ± 4% of all particles, which represents a mass fraction of 4.2 ± 0.1% on average.
29052468	2	28	theme	estimated	269:277	arg1	intakes					279:285	the estimated intakes	265:285	the estimated intakes of TiO2 nanoparticles	265:307	Due to differences in the mass fraction of nanoparticles contained in TiO2, the estimated intakes of TiO2 nanoparticles differ by a factor of 10 in the literature.
29052468	6	29	theme	chemical	949:956	arg1	composition					958:968	their chemical composition	943:968	their chemical composition	943:968	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	1	30	theme	many	142:145	arg1	categories					152:161	many food categories	142:161	many food categories	142:161	Titanium dioxide is a metal oxide used as a white pigment in many food categories, including confectionery.
29052468	1	30	theme	many	142:145	arg1	confectionery					174:186	confectionery	174:186	confectionery	174:186	Titanium dioxide is a metal oxide used as a white pigment in many food categories, including confectionery.
29052468	9	31	theme	17.3 ± 0.9 mg	1396:1408	arg1	particles					1415:1423	17.3 ± 0.9 mg TiO2 particles	1396:1423	17.3 ± 0.9 mg TiO2 particles per piece of chewing gum	1396:1448	We found 1.1 ± 0.3 to 17.3 ± 0.9 mg TiO2 particles per piece of chewing gum, with a mean diameter of 135 ± 42 nm.
29052468	8	32	theme	gums	1219:1222	arg1	coating					1195:1201	the entire coating	1184:1201	the entire coating of some chewing gums	1184:1222	TiO2 particles constitute the entire coating of some chewing gums, whereas for others, TiO2 particles are embedded in an organic matrix and/or mixed with minerals like calcium carbonate, talc, or magnesium silicate.
29052468	1	33	theme	food	147:150	arg1	categories					152:161	many food categories	142:161	many food categories	142:161	Titanium dioxide is a metal oxide used as a white pigment in many food categories, including confectionery.
29052468	1	33	theme	food	147:150	arg1	confectionery					174:186	confectionery	174:186	confectionery	174:186	Titanium dioxide is a metal oxide used as a white pigment in many food categories, including confectionery.
29052468	0	34	theme	content	18:24	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the content of TiO2	0:32	Evaluation of the content of TiO2 nanoparticles in the coatings of chewing gums.
29052468	8	35	theme	TiO2	1158:1161	arg1	particles					1163:1171	TiO2 particles	1158:1171	TiO2 particles	1158:1171	TiO2 particles constitute the entire coating of some chewing gums, whereas for others, TiO2 particles are embedded in an organic matrix and/or mixed with minerals like calcium carbonate, talc, or magnesium silicate.
29052468	3	36	from	present	427:433	arg1	products					443:450	food products	438:450	food products	438:450	To resolve this problem, a better estimation of the mass of nanoparticles present in food products is needed.
29052468	1	37	theme	Titanium	81:88	arg1	oxide					109:113	a metal oxide	101:113	a metal oxide used as a white pigment in many food categories, including confectionery	101:186	Titanium dioxide is a metal oxide used as a white pigment in many food categories, including confectionery.
29052468	1	37	theme	Titanium	81:88	arg1	dioxide					90:96	Titanium dioxide	81:96	Titanium dioxide	81:96	Titanium dioxide is a metal oxide used as a white pigment in many food categories, including confectionery.
29052468	10	38	theme	TiO2	1488:1491	arg1	nanoparticles					1493:1505	TiO2 nanoparticles	1488:1505	TiO2 nanoparticles	1488:1505	TiO2 nanoparticles account for 19 ± 4% of all particles, which represents a mass fraction of 4.2 ± 0.1% on average.
29052468	7	39	theme	chewing	1033:1039	arg1	gums					1041:1044	the four chewing gums	1024:1044	the four chewing gums	1024:1044	The coatings of the four chewing gums differ chemically from each other, and more specifically the amount of TiO2 varies from one coating to another.
29052468	8	40	theme	chewing	1211:1217	arg1	gums					1219:1222	some chewing gums	1206:1222	some chewing gums	1206:1222	TiO2 particles constitute the entire coating of some chewing gums, whereas for others, TiO2 particles are embedded in an organic matrix and/or mixed with minerals like calcium carbonate, talc, or magnesium silicate.
29052468	3	41	theme	food	438:441	arg1	products					443:450	food products	438:450	food products	438:450	To resolve this problem, a better estimation of the mass of nanoparticles present in food products is needed.
29052468	8	42	from	embedded	1264:1271	arg1	matrix					1287:1292	an organic matrix	1276:1292	an organic matrix	1276:1292	TiO2 particles constitute the entire coating of some chewing gums, whereas for others, TiO2 particles are embedded in an organic matrix and/or mixed with minerals like calcium carbonate, talc, or magnesium silicate.
29052468	0	43	theme	TiO2	29:32	arg1	content					18:24	the content	14:24	the content of TiO2	14:32	Evaluation of the content of TiO2 nanoparticles in the coatings of chewing gums.
29052468	3	44	theme	present	427:433	arg1	nanoparticles					413:425	nanoparticles	413:425	nanoparticles present in food products	413:450	To resolve this problem, a better estimation of the mass of nanoparticles present in food products is needed.
29052468	4	45	theme	food	537:540	arg1	products					542:549	the food products	533:549	the food products contributing most to the intake of TiO2	533:589	In this study, we focused our efforts on chewing gum, which is one of the food products contributing most to the intake of TiO2.
29052468	11	46	theme	nano-TiO2	1757:1765	arg1	0.81 ± 0.04 mg					1739:1752	0.81 ± 0.04 mg	1739:1752	0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum	1739:1790	The intake of nanoparticles is thus highly dependent on the kind of chewing gum, with an estimated range extending from 0.04 ± 0.01 to 0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum.
29052468	10	47	from	fraction	1569:1576	arg1	average					1595:1601	average	1595:1601	average	1595:1601	TiO2 nanoparticles account for 19 ± 4% of all particles, which represents a mass fraction of 4.2 ± 0.1% on average.
29052468	3	48	theme	better	380:385	arg1	estimation					387:396	a better estimation	378:396	a better estimation of the mass of nanoparticles present in food products	378:450	To resolve this problem, a better estimation of the mass of nanoparticles present in food products is needed.
29052468	11	49	theme	estimated	1693:1701	arg1	range					1703:1707	an estimated range	1690:1707	an estimated range extending from 0.04 ± 0.01 to 0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum	1690:1790	The intake of nanoparticles is thus highly dependent on the kind of chewing gum, with an estimated range extending from 0.04 ± 0.01 to 0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum.
29052468	6	50	theme	X-ray	803:807	arg1	diffraction					809:819	X-ray diffraction	803:819	X-ray diffraction	803:819	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	12	51	theme	exposure	1831:1838	arg1	scenario					1840:1847	the exposure scenario	1827:1847	the exposure scenario	1827:1847	These data should serve to refine the exposure scenario.
29052468	5	52	theme	Raman	679:683	arg1	spectroscopy					685:696	Raman spectroscopy	679:696	Raman spectroscopy	679:696	The coatings of four kinds of chewing gum, where the presence of TiO2 was confirmed by Raman spectroscopy, were extracted in aqueous phases.
29052468	6	53	theme	emission	898:905	arg1	ICP-AES					921:927	ICP-AES	921:927	ICP-AES	921:927	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	6	53	theme	emission	898:905	arg1	spectroscopy					907:918	inductively coupled plasma atomic emission spectroscopy	864:918	inductively coupled plasma atomic emission spectroscopy (ICP-AES)	864:928	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	5	54	theme	TiO2	657:660	arg1	presence					645:652	the presence	641:652	the presence of TiO2	641:660	The coatings of four kinds of chewing gum, where the presence of TiO2 was confirmed by Raman spectroscopy, were extracted in aqueous phases.
29052468	8	55	dep	constitute	1173:1182	arg1	whereas					1225:1231	whereas	1225:1231	whereas	1225:1231	TiO2 particles constitute the entire coating of some chewing gums, whereas for others, TiO2 particles are embedded in an organic matrix and/or mixed with minerals like calcium carbonate, talc, or magnesium silicate.
29052468	3	56	theme	nanoparticles	413:425	arg1	mass					405:408	the mass	401:408	the mass of nanoparticles present in food products	401:450	To resolve this problem, a better estimation of the mass of nanoparticles present in food products is needed.
29052468	11	57	theme	nanoparticles	1618:1630	arg1	intake					1608:1613	The intake	1604:1613	The intake of nanoparticles	1604:1630	The intake of nanoparticles is thus highly dependent on the kind of chewing gum, with an estimated range extending from 0.04 ± 0.01 to 0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum.
29052468	11	57	theme	nanoparticles	1618:1630	arg1	dependent					1647:1655	dependent	1647:1655	dependent	1647:1655	The intake of nanoparticles is thus highly dependent on the kind of chewing gum, with an estimated range extending from 0.04 ± 0.01 to 0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum.
29052468	8	58	theme	TiO2	1245:1248	arg1	particles					1250:1258	TiO2 particles	1245:1258	TiO2 particles	1245:1258	TiO2 particles constitute the entire coating of some chewing gums, whereas for others, TiO2 particles are embedded in an organic matrix and/or mixed with minerals like calcium carbonate, talc, or magnesium silicate.
29052468	2	59	theme	TiO2	290:293	arg1	nanoparticles					295:307	TiO2 nanoparticles	290:307	TiO2 nanoparticles	290:307	Due to differences in the mass fraction of nanoparticles contained in TiO2, the estimated intakes of TiO2 nanoparticles differ by a factor of 10 in the literature.
29052468	11	60	theme	chewing	1780:1786	arg1	gum					1788:1790	chewing gum	1780:1790	chewing gum	1780:1790	The intake of nanoparticles is thus highly dependent on the kind of chewing gum, with an estimated range extending from 0.04 ± 0.01 to 0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum.
29052468	1	61	theme	metal	103:107	arg1	oxide					109:113	a metal oxide	101:113	a metal oxide used as a white pigment in many food categories, including confectionery	101:186	Titanium dioxide is a metal oxide used as a white pigment in many food categories, including confectionery.
29052468	1	61	theme	metal	103:107	arg1	dioxide					90:96	Titanium dioxide	81:96	Titanium dioxide	81:96	Titanium dioxide is a metal oxide used as a white pigment in many food categories, including confectionery.
29052468	10	62	theme	%	1590:1590	arg1	fraction					1569:1576	a mass fraction	1562:1576	a mass fraction of 4.2 ± 0.1% on average	1562:1601	TiO2 nanoparticles account for 19 ± 4% of all particles, which represents a mass fraction of 4.2 ± 0.1% on average.
29052468	6	63	theme	atomic	891:896	arg1	ICP-AES					921:927	ICP-AES	921:927	ICP-AES	921:927	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	6	63	theme	atomic	891:896	arg1	spectroscopy					907:918	inductively coupled plasma atomic emission spectroscopy	864:918	inductively coupled plasma atomic emission spectroscopy (ICP-AES)	864:928	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	8	64	theme	calcium	1326:1332	arg1	carbonate					1334:1342	calcium carbonate	1326:1342	calcium carbonate	1326:1342	TiO2 particles constitute the entire coating of some chewing gums, whereas for others, TiO2 particles are embedded in an organic matrix and/or mixed with minerals like calcium carbonate, talc, or magnesium silicate.
29052468	2	65	theme	mass	215:218	arg1	fraction					220:227	the mass fraction	211:227	the mass fraction of nanoparticles contained in TiO2	211:262	Due to differences in the mass fraction of nanoparticles contained in TiO2, the estimated intakes of TiO2 nanoparticles differ by a factor of 10 in the literature.
29052468	3	66	attach	present	427:433	arg1	products					443:450	food products	438:450	food products	438:450	To resolve this problem, a better estimation of the mass of nanoparticles present in food products is needed.
29052468	3	66	attach	present	427:433	arg2	nanoparticles					413:425	nanoparticles	413:425	nanoparticles present in food products	413:450	To resolve this problem, a better estimation of the mass of nanoparticles present in food products is needed.
29052468	11	67	theme	gum	1788:1790	arg1	piece					1771:1775	piece	1771:1775	piece of chewing gum	1771:1790	The intake of nanoparticles is thus highly dependent on the kind of chewing gum, with an estimated range extending from 0.04 ± 0.01 to 0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum.
29052468	3	68	theme	mass	405:408	arg1	estimation					387:396	a better estimation	378:396	a better estimation of the mass of nanoparticles present in food products	378:450	To resolve this problem, a better estimation of the mass of nanoparticles present in food products is needed.
29052468	5	69	theme	gum	630:632	arg1	kinds					613:617	four kinds	608:617	four kinds of chewing gum	608:632	The coatings of four kinds of chewing gum, where the presence of TiO2 was confirmed by Raman spectroscopy, were extracted in aqueous phases.
29052468	6	70	dep	Fourier	822:828	arg1	Transform					830:838	Transform	830:838	Transform Raman spectroscopy	830:857	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	11	71	theme	gum	1680:1682	arg1	kind					1664:1667	the kind	1660:1667	the kind of chewing gum	1660:1682	The intake of nanoparticles is thus highly dependent on the kind of chewing gum, with an estimated range extending from 0.04 ± 0.01 to 0.81 ± 0.04 mg of nano-TiO2 per piece of chewing gum.
29052468	9	72	theme	TiO2	1410:1413	arg1	particles					1415:1423	17.3 ± 0.9 mg TiO2 particles	1396:1423	17.3 ± 0.9 mg TiO2 particles per piece of chewing gum	1396:1448	We found 1.1 ± 0.3 to 17.3 ± 0.9 mg TiO2 particles per piece of chewing gum, with a mean diameter of 135 ± 42 nm.
29052468	6	73	theme	coupled	876:882	arg1	ICP-AES					921:927	ICP-AES	921:927	ICP-AES	921:927	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	6	73	theme	coupled	876:882	arg1	spectroscopy					907:918	inductively coupled plasma atomic emission spectroscopy	864:918	inductively coupled plasma atomic emission spectroscopy (ICP-AES)	864:928	The extracts were analysed by transmission electron microscopy (TEM), X-ray diffraction, Fourier Transform Raman spectroscopy, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) to establish their chemical composition, crystallinity and size distribution.
29052468	9	74	theme	mean	1458:1461	arg1	diameter					1463:1470	a mean diameter	1456:1470	a mean diameter of 135 ± 42 nm	1456:1485	We found 1.1 ± 0.3 to 17.3 ± 0.9 mg TiO2 particles per piece of chewing gum, with a mean diameter of 135 ± 42 nm.
29052468	1	75	from	pigment	131:137	arg1	categories					152:161	many food categories	142:161	many food categories	142:161	Titanium dioxide is a metal oxide used as a white pigment in many food categories, including confectionery.
29052468	1	75	from	pigment	131:137	arg1	confectionery					174:186	confectionery	174:186	confectionery	174:186	Titanium dioxide is a metal oxide used as a white pigment in many food categories, including confectionery.
29052468	7	76	theme	TiO2	1117:1120	arg1	TiO2					1117:1120	TiO2	1117:1120	TiO2	1117:1120	The coatings of the four chewing gums differ chemically from each other, and more specifically the amount of TiO2 varies from one coating to another.
29052468	7	76	theme	TiO2	1117:1120	arg1	amount					1107:1112	the amount	1103:1112	the amount of TiO2	1103:1120	The coatings of the four chewing gums differ chemically from each other, and more specifically the amount of TiO2 varies from one coating to another.
29052468	9	77	theme	gum	1446:1448	arg1	piece					1429:1433	piece	1429:1433	piece of chewing gum	1429:1448	We found 1.1 ± 0.3 to 17.3 ± 0.9 mg TiO2 particles per piece of chewing gum, with a mean diameter of 135 ± 42 nm.
29052468	5	78	theme	kinds	613:617	arg1	coatings					596:603	The coatings	592:603	The coatings	592:603	The coatings of four kinds of chewing gum, where the presence of TiO2 was confirmed by Raman spectroscopy, were extracted in aqueous phases.
25999693	7	0	theme	analysis	1222:1229	arg1	results					1237:1243	thermal gravimetric analysis (TGA) results	1202:1243	thermal gravimetric analysis (TGA) results	1202:1243	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	2	1	theme	various	335:341	arg1	concentrations					343:356	various concentrations	335:356	various concentrations of bovine serum albumin (BSA)	335:386	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	1	2	theme	microspheres	250:261	arg1	formation					221:229	the formation	217:229	the formation of SF-CS composite microspheres	217:261	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	7	3	theme	thermal	1202:1208	arg1	results					1237:1243	thermal gravimetric analysis (TGA) results	1202:1243	thermal gravimetric analysis (TGA) results	1202:1243	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	8	4	theme	%	1732:1732	arg1	%					1738:1738	89.04%±4.68%	1727:1738	89.04%±4.68% in 21 days	1727:1749	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	9	5	theme	1st	1873:1875	arg1	day					1877:1879	the 1st day	1869:1879	the 1st day	1869:1879	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	8	6	from	%	1706:1706	arg1	days					1746:1749	21 days	1743:1749	21 days	1743:1749	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	4	7	theme	%	922:922	arg1	%					928:928	50.16%±4.32%	917:928	50.16%±4.32%	917:928	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	8	8	dep	%	1599:1599	arg1	%					1631:1631	±0.96%	1626:1631	±0.96%	1626:1631	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	7	9	theme	FTIR	1469:1472	arg1	data					1474:1477	the TGA and FTIR data	1457:1477	data	1474:1477	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	4	10	theme	%	928:928	arg1	efficiencies					901:912	encapsulation efficiencies	887:912	encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively	887:974	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	3	11	theme	g	683:683	arg1	genipin					685:691	0.5 g genipin	679:691	0.5 g genipin	679:691	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	8	12	theme	mg	1564:1565	arg1	presence					1531:1538	the presence	1527:1538	the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day	1527:1667	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	2	13	theme	CS	305:306	arg1	ratio					308:312	an SF:CS ratio	299:312	an SF:CS ratio of 1:1	299:319	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	7	14	theme	diffraction	1342:1352	arg1	results					1354:1360	X-ray diffraction results	1336:1360	X-ray diffraction results	1336:1360	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	7	15	theme	TGA	1461:1463	arg1	data					1474:1477	the TGA and FTIR data	1457:1477	data	1474:1477	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	4	16	theme	encapsulation	887:899	arg1	efficiencies					901:912	encapsulation efficiencies	887:912	encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively	887:974	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	8	17	theme	BSA	1570:1572	arg1	BSA					1650:1652	the entrapped BSA	1636:1652	the entrapped BSA	1636:1652	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	17	theme	BSA	1570:1572	arg1	%					1599:1599	BSA burst release 30.79%±3.43%	1570:1599	BSA burst release 30.79%±3.43%	1570:1599	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	2	18	theme	SF	302:303	arg1	ratio					308:312	an SF:CS ratio	299:312	an SF:CS ratio of 1:1	299:319	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	4	19	theme	1:1	792:794	arg1	ratio					802:806	1:1 CS:SF ratio	792:806	1:1 CS:SF ratio	792:806	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	10	20	theme	composite	1972:1980	arg1	microspheres					1982:1993	The SF-CS composite microspheres	1962:1993	The SF-CS composite microspheres	1962:1993	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	8	21	from	%	1738:1738	arg1	days					1746:1749	21 days	1743:1749	21 days	1743:1749	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	22	theme	release	1580:1586	arg1	BSA					1650:1652	the entrapped BSA	1636:1652	the entrapped BSA	1636:1652	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	22	theme	release	1580:1586	arg1	%					1599:1599	BSA burst release 30.79%±3.43%	1570:1599	BSA burst release 30.79%±3.43%	1570:1599	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	3	23	theme	CS	694:695	arg1	ratio					647:651	147 μm: mass ratio	634:651	147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin	634:757	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	8	24	theme	%	1593:1593	arg1	BSA					1650:1652	the entrapped BSA	1636:1652	the entrapped BSA	1636:1652	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	24	theme	%	1593:1593	arg1	%					1599:1599	BSA burst release 30.79%±3.43%	1570:1599	BSA burst release 30.79%±3.43%	1570:1599	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	9	25	from	mg	1822:1823	arg1	day					1877:1879	the 1st day	1869:1879	the 1st day	1869:1879	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	3	26	theme	CS	733:734	arg1	ratio					647:651	147 μm: mass ratio	634:651	147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin	634:757	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	4	27	theme	42.19	949:953	arg1	%					960:960	42.19%±7.47%	949:960	42.19%±7.47%	949:960	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	10	28	theme	sustained	2012:2020	arg1	release					2022:2028	higher sustained release	2005:2028	higher sustained release	2005:2028	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	8	29	theme	%	1599:1599	arg1	mg					1553:1554	20 mg	1550:1554	20 mg	1550:1554	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	29	theme	%	1599:1599	arg1	mg					1564:1565	50 mg	1561:1565	50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day	1561:1667	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	29	theme	%	1599:1599	arg1	mg					1546:1547	10 mg	1543:1547	10 mg	1543:1547	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	9	30	theme	encapsulated	1933:1944	arg1	BSA					1946:1948	the total encapsulated BSA	1923:1948	the total encapsulated BSA	1923:1948	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	2	31	theme	CS	484:485	arg1	microspheres					487:498	pure CS microspheres	479:498	pure CS microspheres	479:498	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	4	32	theme	56.58	931:935	arg1	%					942:942	56.58%±3.58%	931:942	56.58%±3.58%	931:942	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	8	33	from	mg	1546:1547	arg1	day					1665:1667	the 1st day	1657:1667	the 1st day	1657:1667	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	3	34	theme	=1:1	700:703	arg1	ratio					647:651	147 μm: mass ratio	634:651	147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin	634:757	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	8	35	theme	34.41	1602:1606	arg1	BSA					1650:1652	the entrapped BSA	1636:1652	the entrapped BSA	1636:1652	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	35	theme	34.41	1602:1606	arg1	%					1613:1613	34.41%±4.46%	1602:1613	34.41%±4.46%	1602:1613	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	36	theme	sustained-release	1484:1500	arg1	microspheres					1502:1513	The sustained-release microspheres	1480:1513	The sustained-release microspheres	1480:1513	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	1	37	theme	fibroin	180:186	arg1	ratios					207:212	genipin and silk fibroin (SF):chitosan (CS) ratios	163:212	genipin and silk fibroin (SF):chitosan (CS) ratios	163:212	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	7	38	theme	noncrystalline	1413:1426	arg1	form					1428:1431	a noncrystalline form	1411:1431	a noncrystalline form	1411:1431	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	3	39	theme	g	718:718	arg1	genipin					720:726	1 g genipin	716:726	1 g genipin	716:726	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	3	40	theme	SF	736:737	arg1	=1:2					739:742	SF =1:2	736:742	SF =1:2	736:742	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	10	41	dep	did	2035:2037	arg1	than					2030:2033	than	2030:2033	than	2030:2033	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	3	42	dep	CS	733:734	arg1	=1:2					739:742	SF =1:2	736:742	SF =1:2	736:742	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	8	43	theme	BSA	1650:1652	arg1	BSA					1650:1652	the entrapped BSA	1636:1652	the entrapped BSA	1636:1652	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	43	theme	BSA	1650:1652	arg1	%					1613:1613	34.41%±4.46%	1602:1613	34.41%±4.46%	1602:1613	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	43	theme	BSA	1650:1652	arg1	%					1599:1599	BSA burst release 30.79%±3.43%	1570:1599	BSA burst release 30.79%±3.43%	1570:1599	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	43	theme	BSA	1650:1652	arg1	%					1625:1625	41.75%	1620:1625	41.75%	1620:1625	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	3	44	theme	g	749:749	arg1	CS					733:734	CS	733:734	CS	733:734	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	3	44	theme	g	749:749	arg1	genipin					751:757	0.5 g genipin	745:757	0.5 g genipin	745:757	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	8	45	theme	1st	1661:1663	arg1	day					1665:1667	the 1st day	1657:1667	the 1st day	1657:1667	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	4	46	theme	mg	849:850	arg1	presence					834:841	the presence	830:841	the presence of 10 mg, 20 mg, and 50 mg of BSA	830:875	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	2	47	theme	albumin	374:380	arg1	concentrations					343:356	various concentrations	335:356	various concentrations of bovine serum albumin (BSA)	335:386	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	8	48	theme	mg	1546:1547	arg1	presence					1531:1538	the presence	1527:1538	the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day	1527:1667	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	9	49	theme	BSA	1946:1948	arg1	BSA					1946:1948	the total encapsulated BSA	1923:1948	the total encapsulated BSA	1923:1948	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	9	49	theme	BSA	1946:1948	arg1	%					1918:1918	83.57%±2.33%	1907:1918	83.57%±2.33% of the total encapsulated BSA	1907:1948	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	10	50	theme	composite	2080:2088	arg1	carrier					2137:2143	a superior drug carrier	2121:2143	a superior drug carrier	2121:2143	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	10	50	theme	composite	2080:2088	arg1	microspheres					2090:2101	these composite microspheres	2074:2101	these composite microspheres	2074:2101	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	9	51	theme	mg	1822:1823	arg1	presence					1807:1814	the presence	1803:1814	the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day	1803:1879	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	1	52	theme	distinct	136:143	arg1	concentrations					145:158	distinct concentrations	136:158	distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios	136:212	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	10	53	theme	CS	2048:2049	arg1	microspheres					2051:2062	the pure CS microspheres	2039:2062	the pure CS microspheres	2039:2062	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	10	54	theme	superior	2123:2130	arg1	carrier					2137:2143	a superior drug carrier	2121:2143	a superior drug carrier	2121:2143	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	10	54	theme	superior	2123:2130	arg1	microspheres					2090:2101	these composite microspheres	2074:2101	these composite microspheres	2074:2101	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	9	55	theme	BSA	1828:1830	arg1	BSA					1862:1864	BSA	1862:1864	BSA	1862:1864	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	9	55	theme	BSA	1828:1830	arg1	%					1857:1857	BSA burst release 39.53%±1.76%	1828:1857	BSA burst release 39.53%±1.76% of BSA	1828:1864	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	0	56	theme	genipin-cross-linked	36:55	arg1	silk					57:60	genipin-cross-linked silk	36:60	genipin-cross-linked silk	36:60	Preparation and characterization of genipin-cross-linked silk fibroin/chitosan sustained-release microspheres.
25999693	8	57	theme	entrapped	1640:1648	arg1	BSA					1650:1652	the entrapped BSA	1636:1652	the entrapped BSA	1636:1652	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	58	theme	%	1700:1700	arg1	%					1706:1706	75.20%±2.52%	1695:1706	75.20%±2.52%	1695:1706	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	2	59	theme	sustained-release	442:458	arg1	rate					460:463	sustained-release rate	442:463	sustained-release rate	442:463	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	8	60	from	mg	1564:1565	arg1	day					1665:1667	the 1st day	1657:1667	the 1st day	1657:1667	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	9	61	theme	release	1838:1844	arg1	BSA					1862:1864	BSA	1862:1864	BSA	1862:1864	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	9	61	theme	release	1838:1844	arg1	%					1857:1857	BSA burst release 39.53%±1.76%	1828:1857	BSA burst release 39.53%±1.76% of BSA	1828:1864	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	8	62	from	day	1665:1667	arg1	presence					1531:1538	the presence	1527:1538	the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day	1527:1667	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	2	63	theme	1:1	317:319	arg1	ratio					308:312	an SF:CS ratio	299:312	an SF:CS ratio of 1:1	299:319	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	8	64	theme	79.16	1709:1713	arg1	%					1720:1720	79.16%±4.31%	1709:1720	79.16%±4.31%	1709:1720	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	2	65	theme	bovine	361:366	arg1	BSA					383:385	BSA	383:385	BSA	383:385	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	2	65	theme	bovine	361:366	arg1	albumin					374:380	bovine serum albumin	361:380	bovine serum albumin (BSA)	361:386	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	1	66	from	effects	125:131	arg1	formation					221:229	the formation	217:229	the formation of SF-CS composite microspheres	217:261	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	5	67	theme	infrared	995:1002	arg1	FTIR					1018:1021	FTIR	1018:1021	FTIR	1018:1021	Fourier-transform infrared spectroscopy (FTIR) results showed that SF and CS were cross-linked and that the α-helices and random coils of SF were converted into β-sheets.
25999693	5	67	theme	infrared	995:1002	arg1	spectroscopy					1004:1015	Fourier-transform infrared spectroscopy	977:1015	Fourier-transform infrared spectroscopy (FTIR) results	977:1030	Fourier-transform infrared spectroscopy (FTIR) results showed that SF and CS were cross-linked and that the α-helices and random coils of SF were converted into β-sheets.
25999693	7	68	theme	gravimetric	1210:1220	arg1	results					1237:1243	thermal gravimetric analysis (TGA) results	1202:1243	thermal gravimetric analysis (TGA) results	1202:1243	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	3	69	theme	SF	659:660	arg1	ratio					647:651	147 μm: mass ratio	634:651	147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin	634:757	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	5	70	theme	SF	1115:1116	arg1	coils					1106:1110	the α-helices and random coils	1081:1110	the α-helices and random coils of SF	1081:1116	Fourier-transform infrared spectroscopy (FTIR) results showed that SF and CS were cross-linked and that the α-helices and random coils of SF were converted into β-sheets.
25999693	9	71	theme	BSA	1862:1864	arg1	BSA					1862:1864	BSA	1862:1864	BSA	1862:1864	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	9	71	theme	BSA	1862:1864	arg1	%					1857:1857	BSA burst release 39.53%±1.76%	1828:1857	BSA burst release 39.53%±1.76% of BSA	1828:1864	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	1	72	theme	composite	240:248	arg1	microspheres					250:261	SF-CS composite microspheres	234:261	SF-CS composite microspheres	234:261	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	3	73	theme	CS	656:657	arg1	ratio					647:651	147 μm: mass ratio	634:651	147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin	634:757	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	3	74	theme	following	524:532	arg1	microspheres					549:560	microspheres	549:560	microspheres	549:560	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	3	74	theme	following	524:532	arg1	spherical					574:582	spherical	574:582	spherical	574:582	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	3	74	theme	following	524:532	arg1	groups					539:544	the following five groups	520:544	the following five groups of microspheres	520:560	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	9	75	theme	CS	1775:1776	arg1	microspheres					1778:1789	The pure CS microspheres	1766:1789	The pure CS microspheres	1766:1789	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	3	76	theme	microspheres	549:560	arg1	microspheres					549:560	microspheres	549:560	microspheres	549:560	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	3	76	theme	microspheres	549:560	arg1	spherical					574:582	spherical	574:582	spherical	574:582	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	3	76	theme	microspheres	549:560	arg1	groups					539:544	the following five groups	520:544	the following five groups of microspheres	520:560	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	1	77	theme	chitosan	193:200	arg1	ratios					207:212	genipin and silk fibroin (SF):chitosan (CS) ratios	163:212	genipin and silk fibroin (SF):chitosan (CS) ratios	163:212	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	7	78	theme	X-ray	1336:1340	arg1	results					1354:1360	X-ray diffraction results	1336:1360	X-ray diffraction results	1336:1360	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	8	79	from	%	1720:1720	arg1	days					1746:1749	21 days	1743:1749	21 days	1743:1749	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	80	theme	burst	1574:1578	arg1	BSA					1650:1652	the entrapped BSA	1636:1652	the entrapped BSA	1636:1652	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	80	theme	burst	1574:1578	arg1	%					1599:1599	BSA burst release 30.79%±3.43%	1570:1599	BSA burst release 30.79%±3.43%	1570:1599	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	4	81	theme	50.16	917:921	arg1	%					928:928	50.16%±4.32%	917:928	50.16%±4.32%	917:928	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	1	82	theme	ratios	207:212	arg1	concentrations					145:158	distinct concentrations	136:158	distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios	136:212	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	7	83	theme	FTIR	1318:1321	arg1	results					1323:1329	the FTIR results	1314:1329	the FTIR results	1314:1329	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	10	84	theme	SF-CS	1966:1970	arg1	microspheres					1982:1993	The SF-CS composite microspheres	1962:1993	The SF-CS composite microspheres	1962:1993	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	3	85	theme	SF	697:698	arg1	=1:1					700:703	SF =1:1	697:703	SF =1:1	697:703	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	1	86	theme	concentrations	145:158	arg1	effects					125:131	the effects	121:131	the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres	121:261	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	4	87	theme	%	954:954	arg1	%					960:960	42.19%±7.47%	949:960	42.19%±7.47%	949:960	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	0	88	link	genipin-cross-linked	36:55	arg1	silk					57:60	genipin-cross-linked silk	36:60	genipin-cross-linked silk	36:60	Preparation and characterization of genipin-cross-linked silk fibroin/chitosan sustained-release microspheres.
25999693	9	89	theme	total	1927:1931	arg1	BSA					1946:1948	the total encapsulated BSA	1923:1948	the total encapsulated BSA	1923:1948	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	1	90	theme	genipin	163:169	arg1	ratios					207:212	genipin and silk fibroin (SF):chitosan (CS) ratios	163:212	genipin and silk fibroin (SF):chitosan (CS) ratios	163:212	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	4	91	theme	%	936:936	arg1	%					942:942	56.58%±3.58%	931:942	56.58%±3.58%	931:942	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	10	92	theme	higher	2005:2010	arg1	release					2022:2028	higher sustained release	2005:2028	higher sustained release	2005:2028	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	9	93	theme	%	1851:1851	arg1	BSA					1862:1864	BSA	1862:1864	BSA	1862:1864	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	9	93	theme	%	1851:1851	arg1	%					1857:1857	BSA burst release 39.53%±1.76%	1828:1857	BSA burst release 39.53%±1.76% of BSA	1828:1864	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	8	94	theme	%	1607:1607	arg1	BSA					1650:1652	the entrapped BSA	1636:1652	the entrapped BSA	1636:1652	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	94	theme	%	1607:1607	arg1	%					1613:1613	34.41%±4.46%	1602:1613	34.41%±4.46%	1602:1613	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	3	95	theme	particle	597:604	arg1	sizes					606:610	particle sizes	597:610	particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin	597:757	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	1	96	theme	silk	175:178	arg1	fibroin					180:186	silk fibroin	175:186	silk fibroin (SF)	175:191	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	1	96	theme	silk	175:178	arg1	SF					189:190	SF	189:190	SF	189:190	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	2	97	theme	pure	479:482	arg1	microspheres					487:498	pure CS microspheres	479:498	pure CS microspheres	479:498	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	4	98	theme	%	942:942	arg1	efficiencies					901:912	encapsulation efficiencies	887:912	encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively	887:974	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	10	99	theme	pure	2043:2046	arg1	microspheres					2051:2062	the pure CS microspheres	2039:2062	the pure CS microspheres	2039:2062	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	9	100	theme	pure	1770:1773	arg1	microspheres					1778:1789	The pure CS microspheres	1766:1789	The pure CS microspheres	1766:1789	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	8	101	theme	%	1613:1613	arg1	mg					1553:1554	20 mg	1550:1554	20 mg	1550:1554	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	101	theme	%	1613:1613	arg1	mg					1564:1565	50 mg	1561:1565	50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day	1561:1667	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	101	theme	%	1613:1613	arg1	mg					1546:1547	10 mg	1543:1547	10 mg	1543:1547	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	9	102	from	presence	1807:1814	arg1	day					1877:1879	the 1st day	1869:1879	the 1st day	1869:1879	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	7	103	theme	BSA	1278:1280	arg1	point					1269:1273	the melting point	1257:1273	the melting point of BSA	1257:1280	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	4	104	theme	g	817:817	arg1	genipin					819:825	0.05 g genipin	812:825	0.05 g genipin	812:825	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	8	105	from	presence	1531:1538	arg1	day					1665:1667	the 1st day	1657:1667	the 1st day	1657:1667	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	9	106	theme	%	1912:1912	arg1	BSA					1946:1948	the total encapsulated BSA	1923:1948	the total encapsulated BSA	1923:1948	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	9	106	theme	%	1912:1912	arg1	%					1918:1918	83.57%±2.33%	1907:1918	83.57%±2.33% of the total encapsulated BSA	1907:1948	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	4	107	theme	CS	796:797	arg1	ratio					802:806	1:1 CS:SF ratio	792:806	1:1 CS:SF ratio	792:806	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	8	108	theme	%	1625:1625	arg1	mg					1553:1554	20 mg	1550:1554	20 mg	1550:1554	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	108	theme	%	1625:1625	arg1	mg					1564:1565	50 mg	1561:1565	50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day	1561:1667	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	8	108	theme	%	1625:1625	arg1	mg					1546:1547	10 mg	1543:1547	10 mg	1543:1547	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	4	109	theme	SF	799:800	arg1	ratio					802:806	1:1 CS:SF ratio	792:806	1:1 CS:SF ratio	792:806	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	4	110	theme	%	960:960	arg1	efficiencies					901:912	encapsulation efficiencies	887:912	encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively	887:974	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	5	111	theme	α-helices	1085:1093	arg1	coils					1106:1110	the α-helices and random coils	1081:1110	the α-helices and random coils of SF	1081:1116	Fourier-transform infrared spectroscopy (FTIR) results showed that SF and CS were cross-linked and that the α-helices and random coils of SF were converted into β-sheets.
25999693	2	112	theme	encapsulation	413:425	arg1	efficiency					427:436	their encapsulation efficiency	407:436	their encapsulation efficiency	407:436	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	2	113	theme	serum	368:372	arg1	BSA					383:385	BSA	383:385	BSA	383:385	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	2	113	theme	serum	368:372	arg1	albumin					374:380	bovine serum albumin	361:380	bovine serum albumin (BSA)	361:386	We selected microspheres featuring an SF:CS ratio of 1:1, encapsulated various concentrations of bovine serum albumin (BSA), and then compared their encapsulation efficiency and sustained-release rate with those of pure CS microspheres.
25999693	8	114	theme	75.20	1695:1699	arg1	%					1706:1706	75.20%±2.52%	1695:1706	75.20%±2.52%	1695:1706	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	9	115	theme	%	1857:1857	arg1	mg					1822:1823	10 mg	1819:1823	10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day	1819:1879	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	0	116	theme	silk	57:60	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of genipin-cross-linked silk fibroin/chitosan sustained-release microspheres.
25999693	0	116	theme	silk	57:60	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of genipin-cross-linked silk fibroin/chitosan sustained-release microspheres.
25999693	7	117	theme	melting	1261:1267	arg1	point					1269:1273	the melting point	1257:1273	the melting point of BSA	1257:1280	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	9	118	theme	burst	1832:1836	arg1	BSA					1862:1864	BSA	1862:1864	BSA	1862:1864	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	9	118	theme	burst	1832:1836	arg1	%					1857:1857	BSA burst release 39.53%±1.76%	1828:1857	BSA burst release 39.53%±1.76% of BSA	1828:1864	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	0	119	theme	sustained-release	79:95	arg1	microspheres					97:108	sustained-release microspheres	79:108	sustained-release microspheres	79:108	Preparation and characterization of genipin-cross-linked silk fibroin/chitosan sustained-release microspheres.
25999693	3	120	theme	mass	642:645	arg1	ratio					647:651	147 μm: mass ratio	634:651	147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin	634:757	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	10	121	theme	drug	2132:2135	arg1	carrier					2137:2143	a superior drug carrier	2121:2143	a superior drug carrier	2121:2143	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	10	121	theme	drug	2132:2135	arg1	microspheres					2090:2101	these composite microspheres	2074:2101	these composite microspheres	2074:2101	The SF-CS composite microspheres exhibited higher sustained release than did the pure CS microspheres, and thus these composite microspheres might function as a superior drug carrier.
25999693	8	122	theme	%	1714:1714	arg1	%					1720:1720	79.16%±4.31%	1709:1720	79.16%±4.31%	1709:1720	The sustained-release microspheres prepared in the presence of 10 mg, 20 mg, and 50 mg of BSA burst release 30.79%±3.43%, 34.41%±4.46%, and 41.75%±0.96% of the entrapped BSA on the 1st day and cumulatively released 75.20%±2.52%, 79.16%±4.31%, and 89.04%±4.68% in 21 days, respectively.
25999693	4	123	theme	mg	867:868	arg1	presence					834:841	the presence	830:841	the presence of 10 mg, 20 mg, and 50 mg of BSA	830:875	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	3	124	theme	μm	638:639	arg1	ratio					647:651	147 μm: mass ratio	634:651	147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin	634:757	We determined that the following five groups of microspheres were highly spherical and featured particle sizes ranging from 70 μm to 147 μm: mass ratio of CS:SF =1:0.5, 0.1 g or 0.5 g genipin; CS:SF =1:1, 0.05 g or 1 g genipin; and CS:SF =1:2, 0.5 g genipin.
25999693	5	125	theme	random	1099:1104	arg1	coils					1106:1110	the α-helices and random coils	1081:1110	the α-helices and random coils of SF	1081:1116	Fourier-transform infrared spectroscopy (FTIR) results showed that SF and CS were cross-linked and that the α-helices and random coils of SF were converted into β-sheets.
25999693	5	126	theme	Fourier-transform	977:993	arg1	FTIR					1018:1021	FTIR	1018:1021	FTIR	1018:1021	Fourier-transform infrared spectroscopy (FTIR) results showed that SF and CS were cross-linked and that the α-helices and random coils of SF were converted into β-sheets.
25999693	5	126	theme	Fourier-transform	977:993	arg1	spectroscopy					1004:1015	Fourier-transform infrared spectroscopy	977:1015	Fourier-transform infrared spectroscopy (FTIR) results	977:1030	Fourier-transform infrared spectroscopy (FTIR) results showed that SF and CS were cross-linked and that the α-helices and random coils of SF were converted into β-sheets.
25999693	7	127	theme	TGA	1232:1234	arg1	results					1237:1243	thermal gravimetric analysis (TGA) results	1202:1243	thermal gravimetric analysis (TGA) results	1202:1243	Moreover, thermal gravimetric analysis (TGA) results showed that the melting point of BSA did not change, which confirmed the FTIR results, and X-ray diffraction results showed that BSA was entrapped in microspheres in a noncrystalline form, which further verified the TGA and FTIR data.
25999693	4	128	theme	BSA	873:875	arg1	mg					849:850	10 mg	846:850	10 mg	846:850	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	4	128	theme	BSA	873:875	arg1	mg					856:857	20 mg	853:857	20 mg	853:857	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	4	128	theme	BSA	873:875	arg1	mg					867:868	50 mg	864:868	50 mg of BSA	864:875	The microspheres prepared using 1:1 CS:SF ratio and 0.05 g genipin in the presence of 10 mg, 20 mg, and 50 mg of BSA exhibited encapsulation efficiencies of 50.16%±4.32%, 56.58%±3.58%, and 42.19%±7.47%, respectively.
25999693	1	129	theme	SF-CS	234:238	arg1	microspheres					250:261	SF-CS composite microspheres	234:261	SF-CS composite microspheres	234:261	We report the effects of distinct concentrations of genipin and silk fibroin (SF):chitosan (CS) ratios on the formation of SF-CS composite microspheres.
25999693	9	130	from	day	1877:1879	arg1	presence					1807:1814	the presence	1803:1814	the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day	1803:1879	The pure CS microspheres prepared in the presence of 10 mg of BSA burst release 39.53%±1.76% of BSA on the 1st day and cumulatively released 83.57%±2.33% of the total encapsulated BSA in 21 days.
25999693	5	131	theme	spectroscopy	1004:1015	arg1	results					1024:1030	Fourier-transform infrared spectroscopy (FTIR) results	977:1030	Fourier-transform infrared spectroscopy (FTIR) results	977:1030	Fourier-transform infrared spectroscopy (FTIR) results showed that SF and CS were cross-linked and that the α-helices and random coils of SF were converted into β-sheets.
27875913	2	0	theme	spray	465:469	arg1	methods					478:484	spray drying methods	465:484	spray drying methods	465:484	MEL/β-CD complexation was performed by kneading, co-precipitation and spray drying methods under different molar ratios.
27875913	4	1	theme	wicking	844:850	arg1	properties					852:861	wicking properties	844:861	wicking properties	844:861	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	8	2	theme	wicking	1292:1298	arg1	properties					1300:1309	wicking properties	1292:1309	wicking properties	1292:1309	CS was the dominant factor, affecting friability, disintegration behavior and drug release, while wicking properties were affected by Primojel and its interaction with Primellose.
27875913	9	3	theme	overlay	1402:1408	arg1	plot					1410:1413	the overlay plot	1398:1413	the overlay plot	1398:1413	Therefore, according to the overlay plot, CS was dominant factor in determining the optimum region based on a QbD approach.
27875913	1	4	theme	ODT	334:336	arg1	formulation					338:348	the ODT formulation	330:348	the ODT formulation based on a quality by design (QbD) approach	330:392	The purpose of this research was to develop an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT), using statistical analysis to optimize the ODT formulation based on a quality by design (QbD) approach.
27875913	5	5	theme	complex	939:945	arg1	formation					913:921	formation	913:921	formation of an amorphous complex	913:945	The spray drying method induced formation of an amorphous complex and enhanced solubility and drug release of MEL.
27875913	0	6	theme	statistical	106:116	arg1	analysis					118:125	statistical analysis	106:125	statistical analysis	106:125	Development and optimization of a meloxicam/β-cyclodextrin complex for orally disintegrating tablet using statistical analysis.
27875913	4	7	theme	Primellose	734:743	arg1	variables					787:795	independent variables	775:795	independent variables on tablet friability, disintegration behavior, wicking properties and drug release	775:878	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	7	theme	Primellose	734:743	arg1	influence					711:719	the influence	707:719	the influence of Primojel, Primellose	707:743	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	7	theme	Primellose	734:743	arg1	CS					768:769	CS	768:769	CS	768:769	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	7	theme	Primellose	734:743	arg1	strength					758:765	crushing strength	749:765	crushing strength (CS)	749:770	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	3	8	theme	infrared	534:541	arg1	spectroscopy					543:554	infrared spectroscopy	534:554	infrared spectroscopy	534:554	Fourier transform infrared spectroscopy, X-ray diffraction and thermal analysis were utilized to evaluate the complexes.
27875913	1	9	theme	disintegrating	262:275	arg1	ODT					285:287	ODT	285:287	ODT	285:287	The purpose of this research was to develop an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT), using statistical analysis to optimize the ODT formulation based on a quality by design (QbD) approach.
27875913	1	9	theme	disintegrating	262:275	arg1	tablet					277:282	an orally disintegrating tablet	252:282	an orally disintegrating tablet (ODT)	252:288	The purpose of this research was to develop an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT), using statistical analysis to optimize the ODT formulation based on a quality by design (QbD) approach.
27875913	2	10	theme	different	492:500	arg1	ratios					508:513	different molar ratios	492:513	different molar ratios	492:513	MEL/β-CD complexation was performed by kneading, co-precipitation and spray drying methods under different molar ratios.
27875913	8	11	theme	drug	1272:1275	arg1	release					1277:1283	drug release	1272:1283	drug release	1272:1283	CS was the dominant factor, affecting friability, disintegration behavior and drug release, while wicking properties were affected by Primojel and its interaction with Primellose.
27875913	9	12	theme	dominant	1423:1430	arg1	factor					1432:1437	dominant factor	1423:1437	dominant factor	1423:1437	Therefore, according to the overlay plot, CS was dominant factor in determining the optimum region based on a QbD approach.
27875913	1	13	theme	inclusion	175:183	arg1	complex					185:191	an inclusion complex	172:191	an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT)	172:288	The purpose of this research was to develop an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT), using statistical analysis to optimize the ODT formulation based on a quality by design (QbD) approach.
27875913	7	14	theme	different	1176:1184	arg1	levels					1186:1191	different levels	1176:1191	different levels	1176:1191	Primojel, Primellose and CS showed unique main effects and interactions at different levels.
27875913	4	15	theme	crushing	749:756	arg1	variables					787:795	independent variables	775:795	independent variables on tablet friability, disintegration behavior, wicking properties and drug release	775:878	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	15	theme	crushing	749:756	arg1	influence					711:719	the influence	707:719	the influence of Primojel, Primellose	707:743	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	15	theme	crushing	749:756	arg1	CS					768:769	CS	768:769	CS	768:769	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	15	theme	crushing	749:756	arg1	strength					758:765	crushing strength	749:765	crushing strength (CS)	749:770	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	16	theme	composite	647:655	arg1	design					657:662	A central composite design	637:662	A central composite design (α = 2)	637:670	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	16	theme	composite	647:655	arg1	α = 2					665:669	α = 2	665:669	α = 2	665:669	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	3	17	theme	X-ray	557:561	arg1	diffraction					563:573	X-ray diffraction	557:573	X-ray diffraction	557:573	Fourier transform infrared spectroscopy, X-ray diffraction and thermal analysis were utilized to evaluate the complexes.
27875913	7	18	from	levels	1186:1191	arg1	effects					1148:1154	unique main effects	1136:1154	unique main effects	1136:1154	Primojel, Primellose and CS showed unique main effects and interactions at different levels.
27875913	7	18	from	levels	1186:1191	arg1	interactions					1160:1171	interactions	1160:1171	interactions at different levels	1160:1191	Primojel, Primellose and CS showed unique main effects and interactions at different levels.
27875913	4	19	theme	disintegration	819:832	arg1	behavior					834:841	disintegration behavior	819:841	disintegration behavior	819:841	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	6	20	used	utilized	1059:1066	arg2	analysis					1033:1040	a QbD-based statistical analysis	1009:1040	a QbD-based statistical analysis	1009:1040	Furthermore, a QbD-based statistical analysis was successfully utilized to optimize the ODT formulation.
27875913	1	21	theme	meloxicam	196:204	arg1	β-CD					228:231	β-CD	228:231	β-CD	228:231	The purpose of this research was to develop an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT), using statistical analysis to optimize the ODT formulation based on a quality by design (QbD) approach.
27875913	1	21	theme	meloxicam	196:204	arg1	/β-cyclodextrin					211:225	meloxicam (MEL)/β-cyclodextrin	196:225	meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT)	196:288	The purpose of this research was to develop an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT), using statistical analysis to optimize the ODT formulation based on a quality by design (QbD) approach.
27875913	7	22	theme	main	1143:1146	arg1	effects					1148:1154	unique main effects	1136:1154	unique main effects	1136:1154	Primojel, Primellose and CS showed unique main effects and interactions at different levels.
27875913	9	23	theme	optimum	1458:1464	arg1	region					1466:1471	the optimum region	1454:1471	the optimum region based on a QbD approach	1454:1495	Therefore, according to the overlay plot, CS was dominant factor in determining the optimum region based on a QbD approach.
27875913	8	24	theme	disintegration	1244:1257	arg1	behavior					1259:1266	disintegration behavior	1244:1266	disintegration behavior	1244:1266	CS was the dominant factor, affecting friability, disintegration behavior and drug release, while wicking properties were affected by Primojel and its interaction with Primellose.
27875913	2	25	theme	MEL/β-CD	395:402	arg1	complexation					404:415	MEL/β-CD complexation	395:415	MEL/β-CD complexation	395:415	MEL/β-CD complexation was performed by kneading, co-precipitation and spray drying methods under different molar ratios.
27875913	5	26	theme	amorphous	929:937	arg1	complex					939:945	an amorphous complex	926:945	an amorphous complex	926:945	The spray drying method induced formation of an amorphous complex and enhanced solubility and drug release of MEL.
27875913	6	27	theme	statistical	1021:1031	arg1	analysis					1033:1040	a QbD-based statistical analysis	1009:1040	a QbD-based statistical analysis	1009:1040	Furthermore, a QbD-based statistical analysis was successfully utilized to optimize the ODT formulation.
27875913	0	28	theme	meloxicam/β-cyclodextrin	34:57	arg1	complex					59:65	a meloxicam/β-cyclodextrin complex	32:65	a meloxicam/β-cyclodextrin complex	32:65	Development and optimization of a meloxicam/β-cyclodextrin complex for orally disintegrating tablet using statistical analysis.
27875913	5	29	theme	drying	891:896	arg1	method					898:903	The spray drying method	881:903	The spray drying method	881:903	The spray drying method induced formation of an amorphous complex and enhanced solubility and drug release of MEL.
27875913	6	30	theme	QbD-based	1011:1019	arg1	analysis					1033:1040	a QbD-based statistical analysis	1009:1040	a QbD-based statistical analysis	1009:1040	Furthermore, a QbD-based statistical analysis was successfully utilized to optimize the ODT formulation.
27875913	5	31	theme	MEL	991:993	arg1	release					980:986	drug release	975:986	drug release	975:986	The spray drying method induced formation of an amorphous complex and enhanced solubility and drug release of MEL.
27875913	5	31	theme	MEL	991:993	arg1	solubility					960:969	solubility	960:969	solubility	960:969	The spray drying method induced formation of an amorphous complex and enhanced solubility and drug release of MEL.
27875913	4	32	theme	central	639:645	arg1	design					657:662	A central composite design	637:662	A central composite design (α = 2)	637:670	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	32	theme	central	639:645	arg1	α = 2					665:669	α = 2	665:669	α = 2	665:669	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	7	33	theme	unique	1136:1141	arg1	effects					1148:1154	unique main effects	1136:1154	unique main effects	1136:1154	Primojel, Primellose and CS showed unique main effects and interactions at different levels.
27875913	1	34	theme	design	372:377	arg1	approach					385:392	design (QbD) approach	372:392	design (QbD) approach	372:392	The purpose of this research was to develop an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT), using statistical analysis to optimize the ODT formulation based on a quality by design (QbD) approach.
27875913	4	35	theme	independent	775:785	arg1	variables					787:795	independent variables	775:795	independent variables on tablet friability, disintegration behavior, wicking properties and drug release	775:878	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	35	theme	independent	775:785	arg1	influence					711:719	the influence	707:719	the influence of Primojel, Primellose	707:743	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	35	theme	independent	775:785	arg1	strength					758:765	crushing strength	749:765	crushing strength (CS)	749:770	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	1	36	theme	/β-cyclodextrin	211:225	arg1	complex					185:191	an inclusion complex	172:191	an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT)	172:288	The purpose of this research was to develop an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT), using statistical analysis to optimize the ODT formulation based on a quality by design (QbD) approach.
27875913	8	37	theme	dominant	1205:1212	arg1	factor					1214:1219	the dominant factor	1201:1219	the dominant factor	1201:1219	CS was the dominant factor, affecting friability, disintegration behavior and drug release, while wicking properties were affected by Primojel and its interaction with Primellose.
27875913	8	37	theme	dominant	1205:1212	arg1	CS					1194:1195	CS	1194:1195	CS	1194:1195	CS was the dominant factor, affecting friability, disintegration behavior and drug release, while wicking properties were affected by Primojel and its interaction with Primellose.
27875913	4	38	theme	tablet	800:805	arg1	friability					807:816	tablet friability	800:816	tablet friability	800:816	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	5	39	theme	spray	885:889	arg1	method					898:903	The spray drying method	881:903	The spray drying method	881:903	The spray drying method induced formation of an amorphous complex and enhanced solubility and drug release of MEL.
27875913	0	40	theme	complex	59:65	arg1	optimization					16:27	optimization	16:27	optimization	16:27	Development and optimization of a meloxicam/β-cyclodextrin complex for orally disintegrating tablet using statistical analysis.
27875913	0	40	theme	complex	59:65	arg1	Development					0:10	Development	0:10	Development	0:10	Development and optimization of a meloxicam/β-cyclodextrin complex for orally disintegrating tablet using statistical analysis.
27875913	1	41	theme	statistical	297:307	arg1	analysis					309:316	statistical analysis	297:316	statistical analysis	297:316	The purpose of this research was to develop an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT), using statistical analysis to optimize the ODT formulation based on a quality by design (QbD) approach.
27875913	4	42	theme	drug	867:870	arg1	release					872:878	drug release	867:878	drug release	867:878	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	1	43	theme	QbD	380:382	arg1	approach					385:392	design (QbD) approach	372:392	design (QbD) approach	372:392	The purpose of this research was to develop an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT), using statistical analysis to optimize the ODT formulation based on a quality by design (QbD) approach.
27875913	3	44	theme	thermal	579:585	arg1	analysis					587:594	thermal analysis	579:594	thermal analysis	579:594	Fourier transform infrared spectroscopy, X-ray diffraction and thermal analysis were utilized to evaluate the complexes.
27875913	4	45	from	variables	787:795	arg1	friability					807:816	tablet friability	800:816	tablet friability	800:816	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	45	from	variables	787:795	arg1	behavior					834:841	disintegration behavior	819:841	disintegration behavior	819:841	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	45	from	variables	787:795	arg1	properties					852:861	wicking properties	844:861	wicking properties	844:861	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	4	45	from	variables	787:795	arg1	release					872:878	drug release	867:878	drug release	867:878	A central composite design (α = 2) was applied to optimize and assess the influence of Primojel, Primellose and crushing strength (CS) as independent variables on tablet friability, disintegration behavior, wicking properties and drug release.
27875913	3	46	dep	Fourier	516:522	arg1	transform					524:532	transform	524:532	transform infrared spectroscopy, X-ray diffraction and thermal analysis	524:594	Fourier transform infrared spectroscopy, X-ray diffraction and thermal analysis were utilized to evaluate the complexes.
27875913	8	47	with	interaction	1345:1355	arg1	Primellose					1362:1371	Primellose	1362:1371	Primellose	1362:1371	CS was the dominant factor, affecting friability, disintegration behavior and drug release, while wicking properties were affected by Primojel and its interaction with Primellose.
27875913	5	48	theme	drug	975:978	arg1	release					980:986	drug release	975:986	drug release	975:986	The spray drying method induced formation of an amorphous complex and enhanced solubility and drug release of MEL.
27875913	1	49	theme	research	148:155	arg1	purpose					132:138	The purpose	128:138	The purpose of this research	128:155	The purpose of this research was to develop an inclusion complex of meloxicam (MEL)/β-cyclodextrin (β-CD) incorporated into an orally disintegrating tablet (ODT), using statistical analysis to optimize the ODT formulation based on a quality by design (QbD) approach.
27875913	0	50	theme	disintegrating	78:91	arg1	tablet					93:98	orally disintegrating tablet	71:98	orally disintegrating tablet using statistical analysis	71:125	Development and optimization of a meloxicam/β-cyclodextrin complex for orally disintegrating tablet using statistical analysis.
27875913	6	51	theme	ODT	1084:1086	arg1	formulation					1088:1098	the ODT formulation	1080:1098	the ODT formulation	1080:1098	Furthermore, a QbD-based statistical analysis was successfully utilized to optimize the ODT formulation.
27875913	9	52	theme	QbD	1484:1486	arg1	approach					1488:1495	a QbD approach	1482:1495	a QbD approach	1482:1495	Therefore, according to the overlay plot, CS was dominant factor in determining the optimum region based on a QbD approach.
27875913	8	53	with	Primojel	1328:1335	arg1	Primellose					1362:1371	Primellose	1362:1371	Primellose	1362:1371	CS was the dominant factor, affecting friability, disintegration behavior and drug release, while wicking properties were affected by Primojel and its interaction with Primellose.
27875913	2	54	theme	drying	471:476	arg1	methods					478:484	spray drying methods	465:484	spray drying methods	465:484	MEL/β-CD complexation was performed by kneading, co-precipitation and spray drying methods under different molar ratios.
27875913	3	55	used	utilized	601:608	arg2	Fourier					516:522	Fourier	516:522	Fourier transform infrared spectroscopy, X-ray diffraction and thermal analysis	516:594	Fourier transform infrared spectroscopy, X-ray diffraction and thermal analysis were utilized to evaluate the complexes.
27875913	2	56	theme	molar	502:506	arg1	ratios					508:513	different molar ratios	492:513	different molar ratios	492:513	MEL/β-CD complexation was performed by kneading, co-precipitation and spray drying methods under different molar ratios.
24275561	1	0	theme	cartilage	151:159	arg1	properties					127:136	The mechanical properties	112:136	The mechanical properties of articular cartilage	112:159	The mechanical properties of articular cartilage vary enormously with loading rate, and how these properties derive from the composition and structure of the tissue is still unclear.
24275561	10	1	theme	variance	1313:1320	arg1	%					1304:1304	about 25%	1296:1304	about 25% of the variance in modulus	1296:1331	Composition was not strongly associated with modulus; water and collagen contents together predicted about 25% of the variance in modulus.
24275561	10	1	theme	variance	1313:1320	arg1	variance					1313:1320	the variance	1309:1320	the variance in modulus	1309:1331	Composition was not strongly associated with modulus; water and collagen contents together predicted about 25% of the variance in modulus.
24275561	6	2	theme	dynamic	943:949	arg1	modulus					951:957	The maximum dynamic modulus	931:957	The maximum dynamic modulus	931:957	The maximum dynamic modulus ranged from 25 to 150MPa.
24275561	4	3	theme	loading	818:824	arg1	curves					840:845	the loading and unloading curves	814:845	the loading and unloading curves	814:845	The modulus was calculated from the slope of the loading curve and the coefficient of restitution from the areas under the loading and unloading curves.
24275561	6	4	theme	maximum	935:941	arg1	modulus					951:957	The maximum dynamic modulus	931:957	The maximum dynamic modulus	931:957	The maximum dynamic modulus ranged from 25 to 150MPa.
24275561	2	5	theme	gross	495:499	arg1	composition					501:511	the gross composition	491:511	the gross composition of the tissue	491:525	This study investigates the mechanical properties of human articular cartilage at rapid rates of loading, compares these with measurements at slow rates of loading and explores how they relate to the gross composition of the tissue.
24275561	5	6	theme	glycosaminoglycan	903:919	arg1	composition					855:865	Tissue composition	848:865	Tissue composition	848:865	Tissue composition was measured as water, collagen and glycosaminoglycan contents.
24275561	5	6	theme	glycosaminoglycan	903:919	arg1	contents					921:928	glycosaminoglycan contents	903:928	glycosaminoglycan contents	903:928	Tissue composition was measured as water, collagen and glycosaminoglycan contents.
24275561	2	7	theme	human	348:352	arg1	cartilage					364:372	human articular cartilage	348:372	human articular cartilage	348:372	This study investigates the mechanical properties of human articular cartilage at rapid rates of loading, compares these with measurements at slow rates of loading and explores how they relate to the gross composition of the tissue.
24275561	4	8	theme	restitution	781:791	arg1	slope					731:735	the slope	727:735	the slope of the loading curve	727:756	The modulus was calculated from the slope of the loading curve and the coefficient of restitution from the areas under the loading and unloading curves.
24275561	4	8	theme	restitution	781:791	arg1	coefficient					766:776	the coefficient	762:776	the coefficient of restitution	762:791	The modulus was calculated from the slope of the loading curve and the coefficient of restitution from the areas under the loading and unloading curves.
24275561	2	9	theme	rapid	377:381	arg1	rates					383:387	rapid rates	377:387	rapid rates of loading	377:398	This study investigates the mechanical properties of human articular cartilage at rapid rates of loading, compares these with measurements at slow rates of loading and explores how they relate to the gross composition of the tissue.
24275561	0	10	theme	slow	98:101	arg1	loading					103:109	slow loading	98:109	slow loading	98:109	The mechanical and material properties of elderly human articular cartilage subject to impact and slow loading.
24275561	3	11	theme	velocity	674:681	arg1	1.25ms					683:688	78.5mJ and velocity 1.25ms	663:688	78.5mJ and velocity 1.25ms(-1)	663:692	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	3	11	theme	velocity	674:681	arg1	-1					690:691	-1	690:691	-1	690:691	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	2	12	theme	slow	437:440	arg1	rates					442:446	slow rates	437:446	slow rates of loading	437:457	This study investigates the mechanical properties of human articular cartilage at rapid rates of loading, compares these with measurements at slow rates of loading and explores how they relate to the gross composition of the tissue.
24275561	9	13	theme	Water	1164:1168	arg1	loss					1170:1173	Water loss	1164:1173	Water loss	1164:1173	Water loss was not detectable.
24275561	0	14	theme	mechanical	4:13	arg1	properties					28:37	The mechanical and material properties	0:37	The mechanical and material properties of elderly human articular cartilage	0:74	The mechanical and material properties of elderly human articular cartilage subject to impact and slow loading.
24275561	3	15	theme	1.25ms	683:688	arg1	energy					653:658	an energy	650:658	an energy of 78.5mJ and velocity 1.25ms(-1)	650:692	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	3	16	theme	head	547:550	arg1	biopsies					562:569	Full-depth femoral head cartilage biopsies	528:569	Full-depth femoral head cartilage biopsies	528:569	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	1	17	theme	loading	182:188	arg1	rate					190:193	loading rate	182:193	loading rate	182:193	The mechanical properties of articular cartilage vary enormously with loading rate, and how these properties derive from the composition and structure of the tissue is still unclear.
24275561	8	18	theme	standard	1113:1120	arg1	mean					1107:1110	mean	1107:1110	mean (standard deviation)	1107:1131	The coefficient of restitution was 0.502 (0.066) (mean (standard deviation)) and showed no site variation.
24275561	8	18	theme	standard	1113:1120	arg1	deviation					1122:1130	standard deviation	1113:1130	standard deviation	1113:1130	The coefficient of restitution was 0.502 (0.066) (mean (standard deviation)) and showed no site variation.
24275561	7	19	theme	quasi-static	1035:1046	arg1	loading					1048:1054	quasi-static loading	1035:1054	quasi-static loading	1035:1054	These values compared with 1-3MPa measured during quasi-static loading.
24275561	3	20	theme	cartilage	552:560	arg1	biopsies					562:569	Full-depth femoral head cartilage biopsies	528:569	Full-depth femoral head cartilage biopsies	528:569	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	0	21	theme	material	19:26	arg1	properties					28:37	The mechanical and material properties	0:37	The mechanical and material properties of elderly human articular cartilage	0:74	The mechanical and material properties of elderly human articular cartilage subject to impact and slow loading.
24275561	1	22	theme	tissue	270:275	arg1	structure					253:261	structure	253:261	structure	253:261	The mechanical properties of articular cartilage vary enormously with loading rate, and how these properties derive from the composition and structure of the tissue is still unclear.
24275561	1	22	theme	tissue	270:275	arg1	composition					237:247	composition	237:247	composition	237:247	The mechanical properties of articular cartilage vary enormously with loading rate, and how these properties derive from the composition and structure of the tissue is still unclear.
24275561	3	23	theme	Full-depth	528:537	arg1	biopsies					562:569	Full-depth femoral head cartilage biopsies	528:569	Full-depth femoral head cartilage biopsies	528:569	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	2	24	theme	tissue	520:525	arg1	composition					501:511	the gross composition	491:511	the gross composition of the tissue	491:525	This study investigates the mechanical properties of human articular cartilage at rapid rates of loading, compares these with measurements at slow rates of loading and explores how they relate to the gross composition of the tissue.
24275561	3	25	theme	femoral	539:545	arg1	biopsies					562:569	Full-depth femoral head cartilage biopsies	528:569	Full-depth femoral head cartilage biopsies	528:569	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	3	26	dep	slow	591:594	arg1	unconfined					597:606	unconfined	597:606	unconfined	597:606	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	3	27	from	energy	653:658	arg1	impact					640:645	an impact	637:645	an impact at an energy of 78.5mJ and velocity 1.25ms(-1)	637:692	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	10	28	from	%	1304:1304	arg1	modulus					1325:1331	modulus	1325:1331	modulus	1325:1331	Composition was not strongly associated with modulus; water and collagen contents together predicted about 25% of the variance in modulus.
24275561	2	29	theme	loading	451:457	arg1	rates					442:446	slow rates	437:446	slow rates of loading	437:457	This study investigates the mechanical properties of human articular cartilage at rapid rates of loading, compares these with measurements at slow rates of loading and explores how they relate to the gross composition of the tissue.
24275561	3	30	theme	compression	608:618	arg1	test					620:623	a slow, unconfined compression test	589:623	a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1)	589:692	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	4	31	theme	curve	752:756	arg1	slope					731:735	the slope	727:735	the slope of the loading curve	727:756	The modulus was calculated from the slope of the loading curve and the coefficient of restitution from the areas under the loading and unloading curves.
24275561	4	31	theme	curve	752:756	arg1	coefficient					766:776	the coefficient	762:776	the coefficient of restitution	762:791	The modulus was calculated from the slope of the loading curve and the coefficient of restitution from the areas under the loading and unloading curves.
24275561	2	32	theme	loading	392:398	arg1	rates					383:387	rapid rates	377:387	rapid rates of loading	377:398	This study investigates the mechanical properties of human articular cartilage at rapid rates of loading, compares these with measurements at slow rates of loading and explores how they relate to the gross composition of the tissue.
24275561	0	33	theme	human	50:54	arg1	cartilage					66:74	elderly human articular cartilage	42:74	elderly human articular cartilage	42:74	The mechanical and material properties of elderly human articular cartilage subject to impact and slow loading.
24275561	4	34	theme	unloading	830:838	arg1	curves					840:845	the loading and unloading curves	814:845	the loading and unloading curves	814:845	The modulus was calculated from the slope of the loading curve and the coefficient of restitution from the areas under the loading and unloading curves.
24275561	10	35	theme	collagen	1259:1266	arg1	contents					1268:1275	collagen contents	1259:1275	collagen contents	1259:1275	Composition was not strongly associated with modulus; water and collagen contents together predicted about 25% of the variance in modulus.
24275561	5	36	theme	Tissue	848:853	arg1	composition					855:865	Tissue composition	848:865	Tissue composition	848:865	Tissue composition was measured as water, collagen and glycosaminoglycan contents.
24275561	5	36	theme	Tissue	848:853	arg1	collagen					890:897	collagen	890:897	collagen	890:897	Tissue composition was measured as water, collagen and glycosaminoglycan contents.
24275561	5	36	theme	Tissue	848:853	arg1	contents					921:928	glycosaminoglycan contents	903:928	glycosaminoglycan contents	903:928	Tissue composition was measured as water, collagen and glycosaminoglycan contents.
24275561	5	36	theme	Tissue	848:853	arg1	water					883:887	water	883:887	water	883:887	Tissue composition was measured as water, collagen and glycosaminoglycan contents.
24275561	0	37	theme	elderly	42:48	arg1	cartilage					66:74	elderly human articular cartilage	42:74	elderly human articular cartilage	42:74	The mechanical and material properties of elderly human articular cartilage subject to impact and slow loading.
24275561	8	38	dep	0.066	1099:1103	arg1	mean					1107:1110	mean	1107:1110	mean (standard deviation)	1107:1131	The coefficient of restitution was 0.502 (0.066) (mean (standard deviation)) and showed no site variation.
24275561	8	38	dep	0.066	1099:1103	arg1	deviation					1122:1130	standard deviation	1113:1130	standard deviation	1113:1130	The coefficient of restitution was 0.502 (0.066) (mean (standard deviation)) and showed no site variation.
24275561	2	39	theme	mechanical	323:332	arg1	properties					334:343	the mechanical properties	319:343	the mechanical properties of human articular cartilage	319:372	This study investigates the mechanical properties of human articular cartilage at rapid rates of loading, compares these with measurements at slow rates of loading and explores how they relate to the gross composition of the tissue.
24275561	8	40	theme	restitution	1076:1086	arg1	coefficient					1061:1071	The coefficient	1057:1071	The coefficient of restitution	1057:1086	The coefficient of restitution was 0.502 (0.066) (mean (standard deviation)) and showed no site variation.
24275561	8	40	theme	restitution	1076:1086	arg1	0.066					1099:1103	0.066	1099:1103	0.066	1099:1103	The coefficient of restitution was 0.502 (0.066) (mean (standard deviation)) and showed no site variation.
24275561	1	41	theme	mechanical	116:125	arg1	properties					127:136	The mechanical properties	112:136	The mechanical properties of articular cartilage	112:159	The mechanical properties of articular cartilage vary enormously with loading rate, and how these properties derive from the composition and structure of the tissue is still unclear.
24275561	10	42	from	modulus	1325:1331	arg1	%					1304:1304	about 25%	1296:1304	about 25% of the variance in modulus	1296:1331	Composition was not strongly associated with modulus; water and collagen contents together predicted about 25% of the variance in modulus.
24275561	10	42	from	modulus	1325:1331	arg1	variance					1313:1320	the variance	1309:1320	the variance in modulus	1309:1331	Composition was not strongly associated with modulus; water and collagen contents together predicted about 25% of the variance in modulus.
24275561	1	43	dep	composition	237:247	arg1	the					233:235	the	233:235	the	233:235	The mechanical properties of articular cartilage vary enormously with loading rate, and how these properties derive from the composition and structure of the tissue is still unclear.
24275561	1	44	theme	articular	141:149	arg1	cartilage					151:159	articular cartilage	141:159	articular cartilage	141:159	The mechanical properties of articular cartilage vary enormously with loading rate, and how these properties derive from the composition and structure of the tissue is still unclear.
24275561	8	45	theme	site	1148:1151	arg1	variation					1153:1161	no site variation	1145:1161	no site variation	1145:1161	The coefficient of restitution was 0.502 (0.066) (mean (standard deviation)) and showed no site variation.
24275561	10	46	from	variance	1313:1320	arg1	modulus					1325:1331	modulus	1325:1331	modulus	1325:1331	Composition was not strongly associated with modulus; water and collagen contents together predicted about 25% of the variance in modulus.
24275561	0	47	theme	cartilage	66:74	arg1	properties					28:37	The mechanical and material properties	0:37	The mechanical and material properties of elderly human articular cartilage	0:74	The mechanical and material properties of elderly human articular cartilage subject to impact and slow loading.
24275561	4	48	theme	loading	744:750	arg1	curve					752:756	the loading curve	740:756	the loading curve	740:756	The modulus was calculated from the slope of the loading curve and the coefficient of restitution from the areas under the loading and unloading curves.
24275561	2	49	theme	cartilage	364:372	arg1	properties					334:343	the mechanical properties	319:343	the mechanical properties of human articular cartilage	319:372	This study investigates the mechanical properties of human articular cartilage at rapid rates of loading, compares these with measurements at slow rates of loading and explores how they relate to the gross composition of the tissue.
24275561	0	50	theme	articular	56:64	arg1	cartilage					66:74	elderly human articular cartilage	42:74	elderly human articular cartilage	42:74	The mechanical and material properties of elderly human articular cartilage subject to impact and slow loading.
24275561	3	51	theme	78.5mJ	663:668	arg1	1.25ms					683:688	78.5mJ and velocity 1.25ms	663:688	78.5mJ and velocity 1.25ms(-1)	663:692	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	3	51	theme	78.5mJ	663:668	arg1	-1					690:691	-1	690:691	-1	690:691	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	3	52	theme	slow	591:594	arg1	test					620:623	a slow, unconfined compression test	589:623	a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1)	589:692	Full-depth femoral head cartilage biopsies were subjected to a slow, unconfined compression test followed by an impact at an energy of 78.5mJ and velocity 1.25ms(-1).
24275561	2	53	theme	articular	354:362	arg1	cartilage					364:372	human articular cartilage	348:372	human articular cartilage	348:372	This study investigates the mechanical properties of human articular cartilage at rapid rates of loading, compares these with measurements at slow rates of loading and explores how they relate to the gross composition of the tissue.
24274543	1	0	with	matrices	151:158	arg1	composition					177:187	a different composition	165:187	a different composition	165:187	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	5	1	from	study	771:775	arg1	matrices					803:810	these matrices	797:810	these matrices	797:810	The kinetic study of the release from these matrices had shown a decrease of the initial rate of release (Ri) by reference with water.
24274543	3	2	theme	carbohydrate	451:462	arg1	roles					442:446	The roles	438:446	The roles of carbohydrate (Tragacanth gum) and lipid (oleic acid) on the retention of all the aroma compounds	438:546	The roles of carbohydrate (Tragacanth gum) and lipid (oleic acid) on the retention of all the aroma compounds were pointed out.
24274543	6	3	theme	dispersions	1136:1146	arg1	behaviour					1123:1131	the flow behaviour	1114:1131	the flow behaviour of dispersions	1114:1146	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	1	4	from	matrices	151:158	arg1	diacetyl					133:140	diacetyl	133:140	diacetyl	133:140	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	1	4	from	matrices	151:158	arg1	release					104:110	release	104:110	release	104:110	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	1	4	from	matrices	151:158	arg1	acetate					121:127	ethyl acetate	115:127	ethyl acetate	115:127	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	1	4	from	matrices	151:158	arg1	Partitioning					87:98	Partitioning	87:98	Partitioning	87:98	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	6	5	theme	flow	963:966	arg1	behaviour					968:976	the flow behaviour	959:976	the flow behaviour of all matrices	959:992	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	5	6	from	matrices	803:810	arg1	study					771:775	The kinetic study	759:775	The kinetic study of the release from these matrices	759:810	The kinetic study of the release from these matrices had shown a decrease of the initial rate of release (Ri) by reference with water.
24274543	5	6	from	matrices	803:810	arg1	release					784:790	the release	780:790	the release from these matrices	780:810	The kinetic study of the release from these matrices had shown a decrease of the initial rate of release (Ri) by reference with water.
24274543	3	7	theme	lipid	485:489	arg1	roles					442:446	The roles	438:446	The roles of carbohydrate (Tragacanth gum) and lipid (oleic acid) on the retention of all the aroma compounds	438:546	The roles of carbohydrate (Tragacanth gum) and lipid (oleic acid) on the retention of all the aroma compounds were pointed out.
24274543	5	8	theme	release	856:862	arg1	rate					848:851	the initial rate	836:851	the initial rate of release (Ri)	836:867	The kinetic study of the release from these matrices had shown a decrease of the initial rate of release (Ri) by reference with water.
24274543	4	9	theme	considerable	617:628	arg1	effect					630:635	a considerable effect	615:635	a considerable effect on the thermodynamic component	615:666	Changes in the composition of matrices exhibited a considerable effect on the thermodynamic component as expressed by the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C).
24274543	6	10	theme	matrices	985:992	arg1	behaviour					968:976	the flow behaviour	959:976	the flow behaviour of all matrices	959:992	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	1	11	theme	ethyl	115:119	arg1	acetate					121:127	ethyl acetate	115:127	ethyl acetate	115:127	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	6	12	theme	flow	1118:1121	arg1	behaviour					1123:1131	the flow behaviour	1114:1131	the flow behaviour of dispersions	1114:1146	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	2	13	theme	different	271:279	arg1	composition					281:291	a different composition	269:291	a different composition	269:291	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	5	14	theme	kinetic	763:769	arg1	study					771:775	The kinetic study	759:775	The kinetic study of the release from these matrices	759:810	The kinetic study of the release from these matrices had shown a decrease of the initial rate of release (Ri) by reference with water.
24274543	4	15	theme	partition	699:707	arg1	Ka/l					722:725	Ka/l	722:725	Ka/l	722:725	Changes in the composition of matrices exhibited a considerable effect on the thermodynamic component as expressed by the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C).
24274543	4	15	theme	partition	699:707	arg1	coefficient					709:719	the air/liquid partition coefficient	684:719	the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C)	684:756	Changes in the composition of matrices exhibited a considerable effect on the thermodynamic component as expressed by the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C).
24274543	1	16	theme	different	167:175	arg1	composition					177:187	a different composition	165:187	a different composition	165:187	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	2	17	theme	oleic	415:419	arg1	lipid					408:412	a lipid	406:412	a lipid (oleic acid)	406:425	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	2	17	theme	oleic	415:419	arg1	acid					421:424	oleic acid	415:424	oleic acid	415:424	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	5	18	with	reference	872:880	arg1	water					887:891	water	887:891	water	887:891	The kinetic study of the release from these matrices had shown a decrease of the initial rate of release (Ri) by reference with water.
24274543	2	19	theme	second	371:376	arg1	one					378:380	the second one	367:380	the second one	367:380	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	0	20	theme	Thermodynamic	0:12	arg1	study					26:30	Thermodynamic and kinetic study	0:30	Thermodynamic and kinetic study of volatile compounds in biopolymer based dispersions.	0:85	Thermodynamic and kinetic study of volatile compounds in biopolymer based dispersions.
24274543	3	21	from	roles	442:446	arg1	retention					511:519	the retention	507:519	the retention of all the aroma compounds	507:546	The roles of carbohydrate (Tragacanth gum) and lipid (oleic acid) on the retention of all the aroma compounds were pointed out.
24274543	4	22	from	Changes	566:572	arg1	composition					581:591	the composition	577:591	the composition of matrices	577:603	Changes in the composition of matrices exhibited a considerable effect on the thermodynamic component as expressed by the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C).
24274543	3	23	theme	oleic	492:496	arg1	lipid					485:489	lipid	485:489	lipid (oleic acid)	485:502	The roles of carbohydrate (Tragacanth gum) and lipid (oleic acid) on the retention of all the aroma compounds were pointed out.
24274543	3	23	theme	oleic	492:496	arg1	acid					498:501	oleic acid	492:501	oleic acid	492:501	The roles of carbohydrate (Tragacanth gum) and lipid (oleic acid) on the retention of all the aroma compounds were pointed out.
24274543	6	24	theme	matrices	928:935	arg1	properties					910:919	The rheological properties	894:919	The rheological properties of all matrices	894:935	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	0	25	theme	kinetic	18:24	arg1	study					26:30	Thermodynamic and kinetic study	0:30	Thermodynamic and kinetic study of volatile compounds in biopolymer based dispersions.	0:85	Thermodynamic and kinetic study of volatile compounds in biopolymer based dispersions.
24274543	4	26	theme	coefficient	709:719	arg1	values					728:733	the air/liquid partition coefficient (Ka/l) values	684:733	the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C)	684:756	Changes in the composition of matrices exhibited a considerable effect on the thermodynamic component as expressed by the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C).
24274543	6	27	theme	power	1056:1060	arg1	law					1062:1064	power law	1056:1064	power law model was found as the better model to describe the flow behaviour of dispersions	1056:1146	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	5	28	theme	rate	848:851	arg1	decrease					824:831	a decrease	822:831	a decrease of the initial rate of release (Ri)	822:867	The kinetic study of the release from these matrices had shown a decrease of the initial rate of release (Ri) by reference with water.
24274543	5	29	theme	release	784:790	arg1	study					771:775	The kinetic study	759:775	The kinetic study of the release from these matrices	759:810	The kinetic study of the release from these matrices had shown a decrease of the initial rate of release (Ri) by reference with water.
24274543	0	30	theme	volatile	35:42	arg1	compounds					44:52	volatile compounds	35:52	volatile compounds	35:52	Thermodynamic and kinetic study of volatile compounds in biopolymer based dispersions.
24274543	6	31	dep	law	1062:1064	arg1	found					1076:1080	found	1076:1080	was found as the better model to describe the flow behaviour of dispersions	1072:1146	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	2	32	theme	complex	390:396	arg1	matrix					398:403	called complex matrix	383:403	called complex matrix	383:403	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	6	33	theme	rheological	898:908	arg1	properties					910:919	The rheological properties	894:919	The rheological properties of all matrices	894:935	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	6	34	theme	better	1089:1094	arg1	model					1066:1070	model	1066:1070	model	1066:1070	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	6	34	theme	better	1089:1094	arg1	model					1096:1100	the better model	1085:1100	the better model to describe the flow behaviour of dispersions	1085:1146	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	1	35	theme	static	213:218	arg1	chromatography					235:248	static head space gas chromatography	213:248	static head space gas chromatography	213:248	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	2	36	theme	Tragacanth	344:353	arg1	carbohydrates					329:341	carbohydrates	329:341	carbohydrates (Tragacanth gum)	329:358	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	2	36	theme	Tragacanth	344:353	arg1	gum					355:357	Tragacanth gum	344:357	Tragacanth gum	344:357	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	0	37	theme	compounds	44:52	arg1	study					26:30	Thermodynamic and kinetic study	0:30	Thermodynamic and kinetic study of volatile compounds in biopolymer based dispersions.	0:85	Thermodynamic and kinetic study of volatile compounds in biopolymer based dispersions.
24274543	0	38	from	study	26:30	arg1	dispersions					74:84	biopolymer based dispersions	57:84	biopolymer based dispersions	57:84	Thermodynamic and kinetic study of volatile compounds in biopolymer based dispersions.
24274543	6	39	theme	power	1025:1029	arg1	law					1031:1033	power law	1025:1033	power law	1025:1033	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	4	40	from	equilibrium	738:748	arg1	values					728:733	the air/liquid partition coefficient (Ka/l) values	684:733	the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C)	684:756	Changes in the composition of matrices exhibited a considerable effect on the thermodynamic component as expressed by the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C).
24274543	1	41	theme	acetate	121:127	arg1	release					104:110	release	104:110	release	104:110	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	1	41	theme	acetate	121:127	arg1	Partitioning					87:98	Partitioning	87:98	Partitioning	87:98	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	1	42	from	release	104:110	arg1	matrices					151:158	two matrices	147:158	two matrices with a different composition	147:187	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	2	43	with	matrices	255:262	arg1	composition					281:291	a different composition	269:291	a different composition	269:291	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	3	44	theme	compounds	538:546	arg1	retention					511:519	the retention	507:519	the retention of all the aroma compounds	507:546	The roles of carbohydrate (Tragacanth gum) and lipid (oleic acid) on the retention of all the aroma compounds were pointed out.
24274543	4	45	theme	thermodynamic	644:656	arg1	component					658:666	the thermodynamic component	640:666	the thermodynamic component	640:666	Changes in the composition of matrices exhibited a considerable effect on the thermodynamic component as expressed by the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C).
24274543	5	46	theme	initial	840:846	arg1	rate					848:851	the initial rate	836:851	the initial rate of release (Ri)	836:867	The kinetic study of the release from these matrices had shown a decrease of the initial rate of release (Ri) by reference with water.
24274543	1	47	theme	head	220:223	arg1	chromatography					235:248	static head space gas chromatography	213:248	static head space gas chromatography	213:248	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	6	48	theme	Ellis	1039:1043	arg1	models					1045:1050	Ellis models	1039:1050	Ellis models	1039:1050	The rheological properties of all matrices were investigated and the flow behaviour of all matrices was successfully modelled with power law and Ellis models and power law model was found as the better model to describe the flow behaviour of dispersions.
24274543	4	49	theme	air/liquid	688:697	arg1	Ka/l					722:725	Ka/l	722:725	Ka/l	722:725	Changes in the composition of matrices exhibited a considerable effect on the thermodynamic component as expressed by the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C).
24274543	4	49	theme	air/liquid	688:697	arg1	coefficient					709:719	the air/liquid partition coefficient	684:719	the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C)	684:756	Changes in the composition of matrices exhibited a considerable effect on the thermodynamic component as expressed by the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C).
24274543	1	50	theme	diacetyl	133:140	arg1	release					104:110	release	104:110	release	104:110	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	1	50	theme	diacetyl	133:140	arg1	Partitioning					87:98	Partitioning	87:98	Partitioning	87:98	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	1	51	from	Partitioning	87:98	arg1	matrices					151:158	two matrices	147:158	two matrices with a different composition	147:187	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	1	52	theme	space	225:229	arg1	chromatography					235:248	static head space gas chromatography	213:248	static head space gas chromatography	213:248	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	0	53	theme	based	68:72	arg1	dispersions					74:84	biopolymer based dispersions	57:84	biopolymer based dispersions	57:84	Thermodynamic and kinetic study of volatile compounds in biopolymer based dispersions.
24274543	2	54	theme	called	383:388	arg1	matrix					398:403	called complex matrix	383:403	called complex matrix	383:403	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	3	55	theme	Tragacanth	465:474	arg1	carbohydrate					451:462	carbohydrate	451:462	carbohydrate (Tragacanth gum)	451:479	The roles of carbohydrate (Tragacanth gum) and lipid (oleic acid) on the retention of all the aroma compounds were pointed out.
24274543	3	55	theme	Tragacanth	465:474	arg1	gum					476:478	Tragacanth gum	465:478	Tragacanth gum	465:478	The roles of carbohydrate (Tragacanth gum) and lipid (oleic acid) on the retention of all the aroma compounds were pointed out.
24274543	4	56	from	effect	630:635	arg1	component					658:666	the thermodynamic component	640:666	the thermodynamic component	640:666	Changes in the composition of matrices exhibited a considerable effect on the thermodynamic component as expressed by the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C).
24274543	1	57	theme	gas	231:233	arg1	chromatography					235:248	static head space gas chromatography	213:248	static head space gas chromatography	213:248	Partitioning and release of ethyl acetate and diacetyl from two matrices with a different composition was studied by applying static head space gas chromatography.
24274543	0	58	theme	biopolymer	57:66	arg1	dispersions					74:84	biopolymer based dispersions	57:84	biopolymer based dispersions	57:84	Thermodynamic and kinetic study of volatile compounds in biopolymer based dispersions.
24274543	2	59	contain	containing	318:327	arg2	matrix					398:403	called complex matrix	383:403	called complex matrix	383:403	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	2	59	contain	containing	318:327	arg2	acid					421:424	oleic acid	415:424	oleic acid	415:424	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	2	59	contain	containing	318:327	arg2	carbohydrates					329:341	carbohydrates	329:341	carbohydrates (Tragacanth gum)	329:358	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	2	59	contain	containing	318:327	arg1	one					314:316	one	314:316	one	314:316	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	2	59	contain	containing	318:327	arg2	gum					355:357	Tragacanth gum	344:357	Tragacanth gum	344:357	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	2	59	contain	containing	318:327	arg2	lipid					408:412	a lipid	406:412	a lipid (oleic acid)	406:425	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	2	59	contain	containing	318:327	arg2	one					378:380	the second one	367:380	the second one	367:380	Two matrices with a different composition have been developed: one containing carbohydrates (Tragacanth gum) and in the second one, called complex matrix, a lipid (oleic acid) was added.
24274543	3	60	theme	aroma	532:536	arg1	compounds					538:546	all the aroma compounds	524:546	all the aroma compounds	524:546	The roles of carbohydrate (Tragacanth gum) and lipid (oleic acid) on the retention of all the aroma compounds were pointed out.
24274543	4	61	theme	matrices	596:603	arg1	composition					581:591	the composition	577:591	the composition of matrices	577:603	Changes in the composition of matrices exhibited a considerable effect on the thermodynamic component as expressed by the air/liquid partition coefficient (Ka/l) values at equilibrium (20 °C).
27770946	0	0	from	skeleton	19:26	arg1	pharmaceutics					49:61	pharmaceutics	49:61	pharmaceutics	49:61	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.
27770946	9	1	theme	known	1457:1461	arg1	properties					1471:1480	the known healing properties	1453:1480	the known healing properties of cysteine	1453:1492	Such a system is attractive because it combines the known healing properties of cysteine with the advantageous potentials of the collagen/proteoglycan network, which can act as biocompatible carrier able to absorb the excess of the wound exudate while releasing the drug.
27770946	2	2	dep	use	271:273	arg1	the					267:269	the	267:269	the	267:269	In this work, we suggest the use and valorization of natural horny skeleton of marine sponges (Porifera, Dictyoceratida) as bio-based dressing for topical drug delivery.
27770946	6	3	theme	key	1058:1060	arg1	temperature					1042:1052	temperature	1042:1052	temperature	1042:1052	To this respect, drying time and temperature are key parameters for skeleton-mediated drug crystallization.
27770946	6	3	theme	key	1058:1060	arg1	parameters					1062:1071	key parameters	1058:1071	key parameters for skeleton-mediated drug crystallization	1058:1114	To this respect, drying time and temperature are key parameters for skeleton-mediated drug crystallization.
27770946	6	3	theme	key	1058:1060	arg1	time					1033:1036	drying time	1026:1036	drying time	1026:1036	To this respect, drying time and temperature are key parameters for skeleton-mediated drug crystallization.
27770946	8	4	theme	high	1222:1225	arg1	percentages					1227:1237	The high percentages	1218:1237	The high percentages of drug	1218:1245	The high percentages of drug are found after absorption into sponge powder and in vitro permeation studies demonstrate that cysteine is released more slowly than the pure drug within 1h.
27770946	10	5	theme	natural	1729:1735	arg1	scaffold					1753:1760	natural sponge skeletal scaffold	1729:1760	natural sponge skeletal scaffold	1729:1760	Furthermore, due to its glycosaminoglycans content, natural sponge skeletal scaffold might act as bioactive-biomimetic carrier regulating the wound healing processes.
27770946	7	6	theme	formulations	1204:1215	arg1	loading					1169:1175	drug loading and permeation profiles	1164:1199	loading	1169:1175	Consequently, this behavior seems to influence drug loading and permeation profiles of formulations.
27770946	7	6	theme	formulations	1204:1215	arg1	profiles					1192:1199	drug loading and permeation profiles	1164:1199	profiles	1192:1199	Consequently, this behavior seems to influence drug loading and permeation profiles of formulations.
27770946	2	7	theme	sponges	328:334	arg1	skeleton					309:316	natural horny skeleton	295:316	natural horny skeleton of marine sponges (Porifera, Dictyoceratida)	295:361	In this work, we suggest the use and valorization of natural horny skeleton of marine sponges (Porifera, Dictyoceratida) as bio-based dressing for topical drug delivery.
27770946	5	8	theme	formulations	995:1006	arg1	suitability					965:975	the suitability	961:975	the suitability of skeleton-based formulations	961:1006	Drug content, SEM analyses and in vitro permeation studies were performed to test the suitability of skeleton-based formulations.
27770946	0	9	theme	drug	99:102	arg1	delivery					104:111	topical drug delivery	91:111	topical drug delivery	91:111	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.
27770946	3	10	theme	skeleton	653:660	arg1	properties					662:671	the collagenic skeleton properties	638:671	the collagenic skeleton properties	638:671	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	7	11	theme	permeation	1181:1190	arg1	profiles					1192:1199	drug loading and permeation profiles	1164:1199	profiles	1192:1199	Consequently, this behavior seems to influence drug loading and permeation profiles of formulations.
27770946	10	12	theme	wound	1819:1823	arg1	healing					1825:1831	wound healing	1819:1831	the wound healing processes	1815:1841	Furthermore, due to its glycosaminoglycans content, natural sponge skeletal scaffold might act as bioactive-biomimetic carrier regulating the wound healing processes.
27770946	3	13	theme	microbiological	572:586	arg1	assessment					596:605	microbiological quality assessment	572:605	microbiological quality assessment	572:605	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	10	14	theme	bioactive-biomimetic	1775:1794	arg1	carrier					1796:1802	bioactive-biomimetic carrier	1775:1802	bioactive-biomimetic carrier regulating the wound healing processes	1775:1841	Furthermore, due to its glycosaminoglycans content, natural sponge skeletal scaffold might act as bioactive-biomimetic carrier regulating the wound healing processes.
27770946	0	15	theme	topical	91:97	arg1	delivery					104:111	topical drug delivery	91:111	topical drug delivery	91:111	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.
27770946	9	16	theme	biocompatible	1582:1594	arg1	carrier					1596:1602	biocompatible carrier	1582:1602	biocompatible carrier	1582:1602	Such a system is attractive because it combines the known healing properties of cysteine with the advantageous potentials of the collagen/proteoglycan network, which can act as biocompatible carrier able to absorb the excess of the wound exudate while releasing the drug.
27770946	3	17	theme	fluid	498:502	arg1	performances					511:522	fluid uptake performances	498:522	fluid uptake performances	498:522	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	3	18	theme	swelling	479:486	arg1	behavior					488:495	swelling behavior	479:495	swelling behavior	479:495	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	5	19	theme	in	910:911	arg1	studies					930:936	in vitro permeation studies	910:936	in vitro permeation studies	910:936	Drug content, SEM analyses and in vitro permeation studies were performed to test the suitability of skeleton-based formulations.
27770946	3	20	dep	glycosaminoglycans	525:542	arg1	glycosaminoglycans					525:542	glycosaminoglycans	525:542	glycosaminoglycans content and composition	525:566	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	3	20	dep	glycosaminoglycans	525:542	arg1	composition					556:566	composition	556:566	composition	556:566	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	3	20	dep	glycosaminoglycans	525:542	arg1	content					544:550	content	544:550	content	544:550	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	5	21	theme	SEM	893:895	arg1	analyses					897:904	SEM analyses	893:904	SEM analyses	893:904	Drug content, SEM analyses and in vitro permeation studies were performed to test the suitability of skeleton-based formulations.
27770946	4	22	theme	drug	826:829	arg1	concentrations					831:844	various drug concentrations	818:844	various drug concentrations	818:844	After grinding and sieving processes, l-cysteine hydrochloride-loaded formulations were designed in form of powder or polymeric film by testing various drug concentrations and different drying parameters.
27770946	5	23	theme	permeation	919:928	arg1	studies					930:936	in vitro permeation studies	910:936	in vitro permeation studies	910:936	Drug content, SEM analyses and in vitro permeation studies were performed to test the suitability of skeleton-based formulations.
27770946	2	24	theme	bio-based	366:374	arg1	dressing					376:383	bio-based dressing	366:383	bio-based dressing for topical drug delivery	366:409	In this work, we suggest the use and valorization of natural horny skeleton of marine sponges (Porifera, Dictyoceratida) as bio-based dressing for topical drug delivery.
27770946	9	25	theme	collagen/proteoglycan	1534:1554	arg1	network					1556:1562	the collagen/proteoglycan network	1530:1562	the collagen/proteoglycan network	1530:1562	Such a system is attractive because it combines the known healing properties of cysteine with the advantageous potentials of the collagen/proteoglycan network, which can act as biocompatible carrier able to absorb the excess of the wound exudate while releasing the drug.
27770946	1	26	from	interest	126:133	arg1	use					142:144	the use	138:144	the use of recyclable and biodegradable natural materials	138:194	The growing interest in the use of recyclable and biodegradable natural materials has become a relevant topic in pharmaceutics.
27770946	4	27	theme	various	818:824	arg1	concentrations					831:844	various drug concentrations	818:844	various drug concentrations	818:844	After grinding and sieving processes, l-cysteine hydrochloride-loaded formulations were designed in form of powder or polymeric film by testing various drug concentrations and different drying parameters.
27770946	10	28	theme	glycosaminoglycans	1701:1718	arg1	content					1720:1726	its glycosaminoglycans content	1697:1726	its glycosaminoglycans content	1697:1726	Furthermore, due to its glycosaminoglycans content, natural sponge skeletal scaffold might act as bioactive-biomimetic carrier regulating the wound healing processes.
27770946	1	29	theme	biodegradable	164:176	arg1	materials					186:194	recyclable and biodegradable natural materials	149:194	recyclable and biodegradable natural materials	149:194	The growing interest in the use of recyclable and biodegradable natural materials has become a relevant topic in pharmaceutics.
27770946	0	30	theme	collagenic	8:17	arg1	skeleton					19:26	Natural collagenic skeleton	0:26	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.	0:112	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.
27770946	2	31	theme	natural	295:301	arg1	skeleton					309:316	natural horny skeleton	295:316	natural horny skeleton of marine sponges (Porifera, Dictyoceratida)	295:361	In this work, we suggest the use and valorization of natural horny skeleton of marine sponges (Porifera, Dictyoceratida) as bio-based dressing for topical drug delivery.
27770946	1	32	theme	natural	178:184	arg1	materials					186:194	recyclable and biodegradable natural materials	149:194	recyclable and biodegradable natural materials	149:194	The growing interest in the use of recyclable and biodegradable natural materials has become a relevant topic in pharmaceutics.
27770946	0	33	theme	Natural	0:6	arg1	skeleton					19:26	Natural collagenic skeleton	0:26	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.	0:112	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.
27770946	7	34	theme	drug	1164:1167	arg1	loading					1169:1175	drug loading and permeation profiles	1164:1199	loading	1169:1175	Consequently, this behavior seems to influence drug loading and permeation profiles of formulations.
27770946	4	35	theme	sieving	693:699	arg1	processes					701:709	grinding and sieving processes	680:709	grinding and sieving processes	680:709	After grinding and sieving processes, l-cysteine hydrochloride-loaded formulations were designed in form of powder or polymeric film by testing various drug concentrations and different drying parameters.
27770946	4	36	theme	powder	782:787	arg1	form					774:777	form	774:777	form of powder or polymeric film	774:805	After grinding and sieving processes, l-cysteine hydrochloride-loaded formulations were designed in form of powder or polymeric film by testing various drug concentrations and different drying parameters.
27770946	1	37	theme	materials	186:194	arg1	use					142:144	the use	138:144	the use of recyclable and biodegradable natural materials	138:194	The growing interest in the use of recyclable and biodegradable natural materials has become a relevant topic in pharmaceutics.
27770946	5	38	theme	Drug	879:882	arg1	content					884:890	Drug content	879:890	Drug content	879:890	Drug content, SEM analyses and in vitro permeation studies were performed to test the suitability of skeleton-based formulations.
27770946	6	39	theme	drying	1026:1031	arg1	temperature					1042:1052	temperature	1042:1052	temperature	1042:1052	To this respect, drying time and temperature are key parameters for skeleton-mediated drug crystallization.
27770946	6	39	theme	drying	1026:1031	arg1	parameters					1062:1071	key parameters	1058:1071	key parameters for skeleton-mediated drug crystallization	1058:1114	To this respect, drying time and temperature are key parameters for skeleton-mediated drug crystallization.
27770946	6	39	theme	drying	1026:1031	arg1	time					1033:1036	drying time	1026:1036	drying time	1026:1036	To this respect, drying time and temperature are key parameters for skeleton-mediated drug crystallization.
27770946	9	40	theme	network	1556:1562	arg1	potentials					1516:1525	the advantageous potentials	1499:1525	the advantageous potentials of the collagen/proteoglycan network, which can act as biocompatible carrier able to absorb the excess of the wound exudate while releasing the drug	1499:1674	Such a system is attractive because it combines the known healing properties of cysteine with the advantageous potentials of the collagen/proteoglycan network, which can act as biocompatible carrier able to absorb the excess of the wound exudate while releasing the drug.
27770946	5	41	theme	skeleton-based	980:993	arg1	formulations					995:1006	skeleton-based formulations	980:1006	skeleton-based formulations	980:1006	Drug content, SEM analyses and in vitro permeation studies were performed to test the suitability of skeleton-based formulations.
27770946	4	42	theme	film	802:805	arg1	form					774:777	form	774:777	form of powder or polymeric film	774:805	After grinding and sieving processes, l-cysteine hydrochloride-loaded formulations were designed in form of powder or polymeric film by testing various drug concentrations and different drying parameters.
27770946	2	43	theme	drug	397:400	arg1	delivery					402:409	topical drug delivery	389:409	topical drug delivery	389:409	In this work, we suggest the use and valorization of natural horny skeleton of marine sponges (Porifera, Dictyoceratida) as bio-based dressing for topical drug delivery.
27770946	9	44	theme	exudate	1643:1649	arg1	excess					1623:1628	the excess	1619:1628	the excess of the wound exudate	1619:1649	Such a system is attractive because it combines the known healing properties of cysteine with the advantageous potentials of the collagen/proteoglycan network, which can act as biocompatible carrier able to absorb the excess of the wound exudate while releasing the drug.
27770946	8	45	dep	in	1297:1298	arg1	vitro					1300:1304	vitro	1300:1304	vitro	1300:1304	The high percentages of drug are found after absorption into sponge powder and in vitro permeation studies demonstrate that cysteine is released more slowly than the pure drug within 1h.
27770946	1	46	theme	recyclable	149:158	arg1	materials					186:194	recyclable and biodegradable natural materials	149:194	recyclable and biodegradable natural materials	149:194	The growing interest in the use of recyclable and biodegradable natural materials has become a relevant topic in pharmaceutics.
27770946	2	47	theme	topical	389:395	arg1	delivery					402:409	topical drug delivery	389:409	topical drug delivery	389:409	In this work, we suggest the use and valorization of natural horny skeleton of marine sponges (Porifera, Dictyoceratida) as bio-based dressing for topical drug delivery.
27770946	0	48	theme	sponges	38:44	arg1	skeleton					19:26	Natural collagenic skeleton	0:26	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.	0:112	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.
27770946	9	49	theme	wound	1637:1641	arg1	exudate					1643:1649	the wound exudate	1633:1649	the wound exudate	1633:1649	Such a system is attractive because it combines the known healing properties of cysteine with the advantageous potentials of the collagen/proteoglycan network, which can act as biocompatible carrier able to absorb the excess of the wound exudate while releasing the drug.
27770946	10	50	theme	skeletal	1744:1751	arg1	scaffold					1753:1760	natural sponge skeletal scaffold	1729:1760	natural sponge skeletal scaffold	1729:1760	Furthermore, due to its glycosaminoglycans content, natural sponge skeletal scaffold might act as bioactive-biomimetic carrier regulating the wound healing processes.
27770946	8	51	theme	pure	1384:1387	arg1	drug					1389:1392	the pure drug	1380:1392	the pure drug	1380:1392	The high percentages of drug are found after absorption into sponge powder and in vitro permeation studies demonstrate that cysteine is released more slowly than the pure drug within 1h.
27770946	4	52	theme	grinding	680:687	arg1	processes					701:709	grinding and sieving processes	680:709	grinding and sieving processes	680:709	After grinding and sieving processes, l-cysteine hydrochloride-loaded formulations were designed in form of powder or polymeric film by testing various drug concentrations and different drying parameters.
27770946	0	53	theme	marine	31:36	arg1	sponges					38:44	marine sponges	31:44	marine sponges	31:44	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.
27770946	9	54	theme	cysteine	1485:1492	arg1	properties					1471:1480	the known healing properties	1453:1480	the known healing properties of cysteine	1453:1492	Such a system is attractive because it combines the known healing properties of cysteine with the advantageous potentials of the collagen/proteoglycan network, which can act as biocompatible carrier able to absorb the excess of the wound exudate while releasing the drug.
27770946	8	55	theme	sponge	1279:1284	arg1	powder					1286:1291	sponge powder	1279:1291	sponge powder	1279:1291	The high percentages of drug are found after absorption into sponge powder and in vitro permeation studies demonstrate that cysteine is released more slowly than the pure drug within 1h.
27770946	1	56	theme	relevant	209:216	arg1	topic					218:222	a relevant topic	207:222	a relevant topic in pharmaceutics	207:239	The growing interest in the use of recyclable and biodegradable natural materials has become a relevant topic in pharmaceutics.
27770946	8	57	theme	drug	1242:1245	arg1	percentages					1227:1237	The high percentages	1218:1237	The high percentages of drug	1218:1245	The high percentages of drug are found after absorption into sponge powder and in vitro permeation studies demonstrate that cysteine is released more slowly than the pure drug within 1h.
27770946	3	58	theme	Biomaterial	412:422	arg1	characterization					424:439	Biomaterial characterization	412:439	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment	412:605	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	5	59	dep	in	910:911	arg1	vitro					913:917	vitro	913:917	vitro	913:917	Drug content, SEM analyses and in vitro permeation studies were performed to test the suitability of skeleton-based formulations.
27770946	9	60	theme	advantageous	1503:1514	arg1	potentials					1516:1525	the advantageous potentials	1499:1525	the advantageous potentials of the collagen/proteoglycan network, which can act as biocompatible carrier able to absorb the excess of the wound exudate while releasing the drug	1499:1674	Such a system is attractive because it combines the known healing properties of cysteine with the advantageous potentials of the collagen/proteoglycan network, which can act as biocompatible carrier able to absorb the excess of the wound exudate while releasing the drug.
27770946	2	61	theme	marine	321:326	arg1	Dictyoceratida					347:360	Dictyoceratida	347:360	Dictyoceratida	347:360	In this work, we suggest the use and valorization of natural horny skeleton of marine sponges (Porifera, Dictyoceratida) as bio-based dressing for topical drug delivery.
27770946	2	61	theme	marine	321:326	arg1	sponges					328:334	marine sponges	321:334	marine sponges (Porifera, Dictyoceratida)	321:361	In this work, we suggest the use and valorization of natural horny skeleton of marine sponges (Porifera, Dictyoceratida) as bio-based dressing for topical drug delivery.
27770946	8	62	theme	in	1297:1298	arg1	studies					1317:1323	in vitro permeation studies	1297:1323	in vitro permeation studies	1297:1323	The high percentages of drug are found after absorption into sponge powder and in vitro permeation studies demonstrate that cysteine is released more slowly than the pure drug within 1h.
27770946	3	63	theme	uptake	504:509	arg1	performances					511:522	fluid uptake performances	498:522	fluid uptake performances	498:522	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	1	64	theme	growing	118:124	arg1	interest					126:133	The growing interest	114:133	The growing interest in the use of recyclable and biodegradable natural materials	114:194	The growing interest in the use of recyclable and biodegradable natural materials has become a relevant topic in pharmaceutics.
27770946	3	65	theme	quality	588:594	arg1	assessment					596:605	microbiological quality assessment	572:605	microbiological quality assessment	572:605	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	1	66	from	topic	218:222	arg1	pharmaceutics					227:239	pharmaceutics	227:239	pharmaceutics	227:239	The growing interest in the use of recyclable and biodegradable natural materials has become a relevant topic in pharmaceutics.
27770946	3	67	theme	collagenic	642:651	arg1	properties					662:671	the collagenic skeleton properties	638:671	the collagenic skeleton properties	638:671	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	2	68	theme	skeleton	309:316	arg1	valorization					279:290	valorization	279:290	valorization	279:290	In this work, we suggest the use and valorization of natural horny skeleton of marine sponges (Porifera, Dictyoceratida) as bio-based dressing for topical drug delivery.
27770946	2	68	theme	skeleton	309:316	arg1	use					271:273	use	271:273	use	271:273	In this work, we suggest the use and valorization of natural horny skeleton of marine sponges (Porifera, Dictyoceratida) as bio-based dressing for topical drug delivery.
27770946	10	69	theme	healing	1825:1831	arg1	processes					1833:1841	the wound healing processes	1815:1841	the wound healing processes	1815:1841	Furthermore, due to its glycosaminoglycans content, natural sponge skeletal scaffold might act as bioactive-biomimetic carrier regulating the wound healing processes.
27770946	6	70	theme	drug	1095:1098	arg1	crystallization					1100:1114	skeleton-mediated drug crystallization	1077:1114	skeleton-mediated drug crystallization	1077:1114	To this respect, drying time and temperature are key parameters for skeleton-mediated drug crystallization.
27770946	0	71	dep	skeleton	19:26	arg1	biomaterial					75:85	Innovative biomaterial	64:85	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.	0:112	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.
27770946	4	72	theme	polymeric	792:800	arg1	film					802:805	polymeric film	792:805	polymeric film	792:805	After grinding and sieving processes, l-cysteine hydrochloride-loaded formulations were designed in form of powder or polymeric film by testing various drug concentrations and different drying parameters.
27770946	2	73	theme	horny	303:307	arg1	skeleton					309:316	natural horny skeleton	295:316	natural horny skeleton of marine sponges (Porifera, Dictyoceratida)	295:361	In this work, we suggest the use and valorization of natural horny skeleton of marine sponges (Porifera, Dictyoceratida) as bio-based dressing for topical drug delivery.
27770946	6	74	theme	skeleton-mediated	1077:1093	arg1	crystallization					1100:1114	skeleton-mediated drug crystallization	1077:1114	skeleton-mediated drug crystallization	1077:1114	To this respect, drying time and temperature are key parameters for skeleton-mediated drug crystallization.
27770946	0	75	theme	Innovative	64:73	arg1	biomaterial					75:85	Innovative biomaterial	64:85	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.	0:112	Natural collagenic skeleton of marine sponges in pharmaceutics: Innovative biomaterial for topical drug delivery.
27770946	10	76	theme	sponge	1737:1742	arg1	scaffold					1753:1760	natural sponge skeletal scaffold	1729:1760	natural sponge skeletal scaffold	1729:1760	Furthermore, due to its glycosaminoglycans content, natural sponge skeletal scaffold might act as bioactive-biomimetic carrier regulating the wound healing processes.
27770946	4	77	theme	drying	860:865	arg1	parameters					867:876	different drying parameters	850:876	different drying parameters	850:876	After grinding and sieving processes, l-cysteine hydrochloride-loaded formulations were designed in form of powder or polymeric film by testing various drug concentrations and different drying parameters.
27770946	3	78	theme	morpho-functional	453:469	arg1	traits					471:476	morpho-functional traits	453:476	morpho-functional traits	453:476	Biomaterial characterization focusing on morpho-functional traits, swelling behavior, fluid uptake performances, glycosaminoglycans content and composition and microbiological quality assessment was carried out to investigate the collagenic skeleton properties.
27770946	4	79	theme	hydrochloride-loaded	723:742	arg1	formulations					744:755	l-cysteine hydrochloride-loaded formulations	712:755	l-cysteine hydrochloride-loaded formulations	712:755	After grinding and sieving processes, l-cysteine hydrochloride-loaded formulations were designed in form of powder or polymeric film by testing various drug concentrations and different drying parameters.
27770946	8	80	theme	permeation	1306:1315	arg1	studies					1317:1323	in vitro permeation studies	1297:1323	in vitro permeation studies	1297:1323	The high percentages of drug are found after absorption into sponge powder and in vitro permeation studies demonstrate that cysteine is released more slowly than the pure drug within 1h.
27770946	9	81	theme	healing	1463:1469	arg1	properties					1471:1480	the known healing properties	1453:1480	the known healing properties of cysteine	1453:1492	Such a system is attractive because it combines the known healing properties of cysteine with the advantageous potentials of the collagen/proteoglycan network, which can act as biocompatible carrier able to absorb the excess of the wound exudate while releasing the drug.
27770946	4	82	theme	different	850:858	arg1	parameters					867:876	different drying parameters	850:876	different drying parameters	850:876	After grinding and sieving processes, l-cysteine hydrochloride-loaded formulations were designed in form of powder or polymeric film by testing various drug concentrations and different drying parameters.
27770946	4	83	theme	l-cysteine	712:721	arg1	formulations					744:755	l-cysteine hydrochloride-loaded formulations	712:755	l-cysteine hydrochloride-loaded formulations	712:755	After grinding and sieving processes, l-cysteine hydrochloride-loaded formulations were designed in form of powder or polymeric film by testing various drug concentrations and different drying parameters.
26767280	2	0	theme	Paris	482:486	arg1	herbs					488:492	Paris herbs	482:492	Paris herbs	482:492	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	1	theme	flavonoids	575:584	arg1	content					533:539	The total content	523:539	The total content of saponins	523:551	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	1	theme	flavonoids	575:584	arg1	content					564:570	the total content	554:570	the total content of flavonoids	554:584	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	1	theme	flavonoids	575:584	arg1	content					600:606	the total content	590:606	the total content of polysaccharides	590:625	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	2	theme	active	708:713	arg1	compositions					724:735	active chemical compositions	708:735	active chemical compositions	708:735	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	3	3	theme	saponin	873:879	arg1	II					881:882	saponin II	873:882	saponin II	873:882	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	6	4	theme	large	1340:1344	arg1	scale					1346:1350	a large scale	1338:1350	a large scale	1338:1350	chinensis could be cultivated on a large scale, but the techniques in artificial cultivation should be studied further.
26767280	7	5	theme	same	1432:1435	arg1	time					1437:1440	the same time	1428:1440	the same time	1428:1440	At the same time,Paris bashanensis and Paris polyphylla var.
26767280	3	6	theme	saponins	1032:1039	arg1	content					1021:1027	the total content	1011:1027	the total content of saponins, the total content of flavonoids and the total content of polysaccharides	1011:1113	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	1	7	theme	active	426:431	arg1	compositions					433:444	active compositions	426:444	active compositions	426:444	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	3	8	theme	content	1021:1027	arg1	analyses					999:1006	the analyses	995:1006	the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides	995:1113	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	9	theme	saponin	862:868	arg1	I					870:870	saponin I	862:870	saponin I	862:870	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	10	theme	total	1082:1086	arg1	content					1088:1094	the total content	1078:1094	the total content of polysaccharides	1078:1113	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	0	11	theme	Different	103:111	arg1	Locations					113:121	Different Locations	103:121	Different Locations of Three Gorges Reservoir]	103:148	[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].
26767280	1	12	theme	different	335:343	arg1	locations					345:353	different locations	335:353	different locations of Three Gorges Reservoir	335:379	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	3	13	theme	total	1015:1019	arg1	content					1021:1027	the total content	1011:1027	the total content of saponins, the total content of flavonoids and the total content of polysaccharides	1011:1113	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	1	14	theme	compositions	433:444	arg1	identification					408:421	the identification	404:421	the identification of active compositions	404:444	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	2	15	from	varieties	794:802	arg1	locations					817:825	different locations	807:825	different locations	807:825	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	3	16	theme	I	870:870	arg1	contents					850:857	the contents	846:857	the contents of saponin I, saponin II, saponin VI and saponin VII	846:910	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	16	theme	I	870:870	arg1	RESULTS					828:834	RESULTS	828:834	RESULTS	828:834	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	16	theme	I	870:870	arg1	various					921:927	various	921:927	various	921:927	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	2	17	theme	HPLC	510:513	arg1	method					515:520	HPLC method	510:520	HPLC method	510:520	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	18	from	content	459:465	arg1	herbs					488:492	Paris herbs	482:492	Paris herbs	482:492	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	19	theme	total	558:562	arg1	content					564:570	the total content	554:570	the total content of flavonoids	554:584	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	20	theme	polysaccharides	611:625	arg1	content					533:539	The total content	523:539	The total content of saponins	523:551	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	20	theme	polysaccharides	611:625	arg1	content					564:570	the total content	554:570	the total content of flavonoids	554:584	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	20	theme	polysaccharides	611:625	arg1	content					600:606	the total content	590:606	the total content of polysaccharides	590:625	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	7	21	dep	var	1481:1483	arg1	time					1437:1440	the same time	1428:1440	the same time	1428:1440	At the same time,Paris bashanensis and Paris polyphylla var.
26767280	7	21	dep	var	1481:1483	arg1	bashanensis					1448:1458	bashanensis	1448:1458	bashanensis	1448:1458	At the same time,Paris bashanensis and Paris polyphylla var.
26767280	7	21	dep	var	1481:1483	arg1	polyphylla					1470:1479	polyphylla	1470:1479	polyphylla	1470:1479	At the same time,Paris bashanensis and Paris polyphylla var.
26767280	3	22	theme	saponin	885:891	arg1	contents					850:857	the contents	846:857	the contents of saponin I, saponin II, saponin VI and saponin VII	846:910	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	22	theme	saponin	885:891	arg1	RESULTS					828:834	RESULTS	828:834	RESULTS	828:834	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	22	theme	saponin	885:891	arg1	various					921:927	various	921:927	various	921:927	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	23	theme	total	1046:1050	arg1	content					1052:1058	the total content	1042:1058	the total content of flavonoids	1042:1072	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	24	theme	content	1052:1058	arg1	content					1021:1027	the total content	1011:1027	the total content of saponins, the total content of flavonoids and the total content of polysaccharides	1011:1113	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	25	from	differences	1180:1190	arg1	cultivars					1205:1213	different cultivars	1195:1213	different cultivars	1195:1213	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	2	26	theme	saponins	470:477	arg1	content					459:465	The content	455:465	The content of saponins in Paris herbs	455:492	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	3	27	theme	II	881:882	arg1	contents					850:857	the contents	846:857	the contents of saponin I, saponin II, saponin VI and saponin VII	846:910	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	27	theme	II	881:882	arg1	RESULTS					828:834	RESULTS	828:834	RESULTS	828:834	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	27	theme	II	881:882	arg1	various					921:927	various	921:927	various	921:927	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	28	from	varieties	948:956	arg1	contents					850:857	the contents	846:857	the contents of saponin I, saponin II, saponin VI and saponin VII	846:910	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	28	from	varieties	948:956	arg1	various					921:927	various	921:927	various	921:927	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	29	theme	chemical	1142:1149	arg1	compositions					1151:1162	the chemical compositions	1138:1162	the chemical compositions	1138:1162	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	1	30	theme	Gorges	364:369	arg1	Reservoir					371:379	Three Gorges Reservoir	358:379	Three Gorges Reservoir	358:379	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	2	31	dep	METHODS	447:453	arg1	measured					498:505	measured	498:505	was measured by HPLC method	494:520	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	3	32	from	located	958:964	arg1	locations					979:987	different locations	969:987	different locations	969:987	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	2	33	theme	ultraviolet-visible	648:666	arg1	spectrophotometry					668:684	ultraviolet-visible spectrophotometry	648:684	ultraviolet-visible spectrophotometry	648:684	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	34	from	locations	817:825	arg1	herbs					775:779	the Paris herbs	765:779	the Paris herbs of different varieties in different locations	765:825	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	3	35	theme	VII	908:910	arg1	contents					850:857	the contents	846:857	the contents of saponin I, saponin II, saponin VI and saponin VII	846:910	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	35	theme	VII	908:910	arg1	RESULTS					828:834	RESULTS	828:834	RESULTS	828:834	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	35	theme	VII	908:910	arg1	various					921:927	various	921:927	various	921:927	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	1	36	theme	thesis	183:188	arg1	basis					190:194	the thesis basis	179:194	the thesis basis for screening the Paris varieties which was suitable for the environments	179:268	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	0	37	theme	Composition	26:36	arg1	[Identification					0:14	[Identification	0:14	[Identification of Active Composition	0:36	[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].
26767280	0	37	theme	Composition	26:36	arg1	Evaluation					50:59	Quality Evaluation	42:59	Quality Evaluation of Paris Herbs	42:74	[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].
26767280	8	38	theme	good	1506:1509	arg1	pseudothibetica					1486:1500	pseudothibetica	1486:1500	pseudothibetica	1486:1500	pseudothibetica are good cultivars for planting.
26767280	8	38	theme	good	1506:1509	arg1	cultivars					1511:1519	good cultivars	1506:1519	good cultivars for planting	1506:1532	pseudothibetica are good cultivars for planting.
26767280	0	39	theme	Gorges	132:137	arg1	Reservoir					139:147	Three Gorges Reservoir]	126:148	Three Gorges Reservoir]	126:148	[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].
26767280	5	40	dep	var	1300:1302	arg1	Paris					1283:1287	Paris	1283:1287	Paris	1283:1287	yunnanensis and Paris polyphylla var.
26767280	5	40	dep	var	1300:1302	arg1	yunnanensis					1267:1277	yunnanensis	1267:1277	yunnanensis	1267:1277	yunnanensis and Paris polyphylla var.
26767280	2	41	theme	herbs	775:779	arg1	comparison					751:760	comparison	751:760	comparison of the Paris herbs of different varieties in different locations	751:825	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	0	42	theme	Active	19:24	arg1	Composition					26:36	Active Composition	19:36	Active Composition	19:36	[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].
26767280	2	43	theme	total	527:531	arg1	content					533:539	The total content	523:539	The total content of saponins	523:551	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	1	44	theme	Reservoir	371:379	arg1	locations					345:353	different locations	335:353	different locations of Three Gorges Reservoir	335:379	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	5	45	theme	polyphylla	1289:1298	arg1	var					1300:1302	polyphylla var	1289:1302	polyphylla var	1289:1302	yunnanensis and Paris polyphylla var.
26767280	2	46	theme	Paris	769:773	arg1	herbs					775:779	the Paris herbs	765:779	the Paris herbs of different varieties in different locations	765:825	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	3	47	from	various	921:927	arg1	varieties					948:956	different Paris varieties	932:956	different Paris varieties located in different locations	932:987	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	0	48	theme	Quality	42:48	arg1	Evaluation					50:59	Quality Evaluation	42:59	Quality Evaluation of Paris Herbs	42:74	[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].
26767280	3	49	theme	saponin	900:906	arg1	VII					908:910	saponin VII	900:910	saponin VII	900:910	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	1	50	theme	Paris	286:290	arg1	herbs					292:296	Paris herbs	286:296	Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir	286:379	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	0	51	theme	Reservoir	139:147	arg1	Locations					113:121	Different Locations	103:121	Different Locations of Three Gorges Reservoir]	103:148	[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].
26767280	3	52	theme	different	1195:1203	arg1	cultivars					1205:1213	different cultivars	1195:1213	different cultivars	1195:1213	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	1	53	theme	herbs	292:296	arg1	quality					275:281	the quality	271:281	the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir	271:379	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	3	54	theme	different	932:940	arg1	varieties					948:956	different Paris varieties	932:956	different Paris varieties located in different locations	932:987	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	55	theme	flavonoids	1063:1072	arg1	content					1088:1094	the total content	1078:1094	the total content of polysaccharides	1078:1113	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	55	theme	flavonoids	1063:1072	arg1	saponins					1032:1039	saponins	1032:1039	saponins	1032:1039	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	55	theme	flavonoids	1063:1072	arg1	content					1052:1058	the total content	1042:1058	the total content of flavonoids	1042:1072	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	56	theme	polysaccharides	1099:1113	arg1	content					1088:1094	the total content	1078:1094	the total content of polysaccharides	1078:1113	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	56	theme	polysaccharides	1099:1113	arg1	saponins					1032:1039	saponins	1032:1039	saponins	1032:1039	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	56	theme	polysaccharides	1099:1113	arg1	content					1052:1058	the total content	1042:1058	the total content of flavonoids	1042:1072	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	6	57	from	techniques	1361:1370	arg1	cultivation					1386:1396	artificial cultivation	1375:1396	artificial cultivation	1375:1396	chinensis could be cultivated on a large scale, but the techniques in artificial cultivation should be studied further.
26767280	2	58	theme	total	594:598	arg1	content					600:606	the total content	590:606	the total content of polysaccharides	590:625	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	3	59	dep	saponin	885:891	arg1	VI					893:894	VI	893:894	VI	893:894	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	60	theme	obvious	1172:1178	arg1	differences					1180:1190	obvious differences	1172:1190	obvious differences in different cultivars	1172:1213	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	2	61	theme	different	807:815	arg1	locations					817:825	different locations	807:825	different locations	807:825	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	1	62	theme	different	301:309	arg1	varieties					311:319	different varieties	301:319	different varieties cultivated in different locations of Three Gorges Reservoir	301:379	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	2	63	theme	saponins	544:551	arg1	content					533:539	The total content	523:539	The total content of saponins	523:551	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	63	theme	saponins	544:551	arg1	content					564:570	the total content	554:570	the total content of flavonoids	554:584	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	63	theme	saponins	544:551	arg1	content					600:606	the total content	590:606	the total content of polysaccharides	590:625	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	64	used	used	742:745	arg2	contents					696:703	the contents	692:703	the contents of active chemical compositions	692:735	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	3	65	theme	Paris	942:946	arg1	varieties					948:956	different Paris varieties	932:956	different Paris varieties located in different locations	932:987	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	1	66	theme	Paris	214:218	arg1	varieties					220:228	the Paris varieties	210:228	the Paris varieties which was suitable for the environments	210:268	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	1	66	theme	Paris	214:218	arg1	suitable					240:247	suitable	240:247	suitable	240:247	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	3	67	theme	content	1088:1094	arg1	content					1021:1027	the total content	1011:1027	the total content of saponins, the total content of flavonoids and the total content of polysaccharides	1011:1113	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	68	dep	RESULTS	828:834	arg1	Types					836:840	Types	836:840	Types	836:840	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	3	69	theme	located	958:964	arg1	varieties					948:956	different Paris varieties	932:956	different Paris varieties located in different locations	932:987	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	6	70	theme	artificial	1375:1384	arg1	cultivation					1386:1396	artificial cultivation	1375:1396	artificial cultivation	1375:1396	chinensis could be cultivated on a large scale, but the techniques in artificial cultivation should be studied further.
26767280	2	71	theme	varieties	794:802	arg1	herbs					775:779	the Paris herbs	765:779	the Paris herbs of different varieties in different locations	765:825	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	0	72	theme	Herbs	70:74	arg1	[Identification					0:14	[Identification	0:14	[Identification of Active Composition	0:36	[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].
26767280	0	72	theme	Herbs	70:74	arg1	Evaluation					50:59	Quality Evaluation	42:59	Quality Evaluation of Paris Herbs	42:74	[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].
26767280	2	73	theme	compositions	724:735	arg1	contents					696:703	the contents	692:703	the contents of active chemical compositions	692:735	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	74	theme	different	784:792	arg1	varieties					794:802	different varieties	784:802	different varieties in different locations	784:825	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	3	75	theme	different	969:977	arg1	locations					979:987	different locations	969:987	different locations	969:987	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	0	76	theme	Paris	64:68	arg1	Herbs					70:74	Paris Herbs	64:74	Paris Herbs	64:74	[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].
26767280	1	77	theme	varieties	311:319	arg1	herbs					292:296	Paris herbs	286:296	Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir	286:379	OBJECTIVE In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.
26767280	2	78	from	herbs	775:779	arg1	locations					817:825	different locations	807:825	different locations	807:825	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	2	79	theme	chemical	715:722	arg1	compositions					724:735	active chemical compositions	708:735	active chemical compositions	708:735	METHODS The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.
26767280	3	80	from	locations	979:987	arg1	located					958:964	located	958:964	located	958:964	RESULTS Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.
26767280	4	81	dep	CONCLUSION	1216:1225	arg1	proved					1233:1238	proved	1233:1238	is proved that Paris polyphylla var	1230:1264	CONCLUSION It is proved that Paris polyphylla var.
24647364	6	0	theme	hydrogels	941:949	arg1	sugar-responsiveness					913:932	The sugar-responsiveness	909:932	The sugar-responsiveness of the hydrogels	909:949	The sugar-responsiveness of the hydrogels was examined by protein loading and release experiments, and the results indicate that this property is also dependent on the compositions of the gels; at a proper component ratio, a new glucose-responsive hydrogel system operating at physiological pH can be obtained.
24647364	4	1	theme	gelation	701:708	arg1	time					710:713	the gelation time	697:713	the gelation time	697:713	The ratios of the three components have a remarkable effect on the gelation time and the mechanical properties of the final gels.
24647364	1	2	theme	inclusion	176:184	arg1	complexation					186:197	inclusion complexation	176:197	inclusion complexation	176:197	A novel glucose-responsive hydrogel system based on dynamic covalent chemistry and inclusion complexation was described.
24647364	5	3	theme	rheological	767:777	arg1	measurements					779:790	rheological measurements	767:790	rheological measurements	767:790	In rheological measurements, the hydrogels are demonstrated to possess solid-like behaviour and good structural recovery ability after yielding.
24647364	7	4	theme	diabetes	1508:1515	arg1	treatment					1495:1503	treatment	1495:1503	treatment of diabetes	1495:1515	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	7	5	theme	hydrogel	1395:1402	arg1	systems					1404:1410	hydrogel systems	1395:1410	hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes	1395:1515	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	6	6	theme	new	1134:1136	arg1	system					1166:1171	a new glucose-responsive hydrogel system	1132:1171	a new glucose-responsive hydrogel system operating at physiological pH	1132:1201	The sugar-responsiveness of the hydrogels was examined by protein loading and release experiments, and the results indicate that this property is also dependent on the compositions of the gels; at a proper component ratio, a new glucose-responsive hydrogel system operating at physiological pH can be obtained.
24647364	7	7	theme	components	1274:1283	arg1	biocompatibility					1244:1259	good biocompatibility	1239:1259	good biocompatibility of the three components	1239:1283	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	7	7	theme	components	1274:1283	arg1	preparation					1298:1308	the easy preparation	1289:1308	the easy preparation of hydrogels with tunable glucose-responsiveness	1289:1357	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	6	8	theme	hydrogel	1157:1164	arg1	system					1166:1171	a new glucose-responsive hydrogel system	1132:1171	a new glucose-responsive hydrogel system operating at physiological pH	1132:1201	The sugar-responsiveness of the hydrogels was examined by protein loading and release experiments, and the results indicate that this property is also dependent on the compositions of the gels; at a proper component ratio, a new glucose-responsive hydrogel system operating at physiological pH can be obtained.
24647364	4	9	theme	gels	758:761	arg1	properties					734:743	the mechanical properties	719:743	the mechanical properties of the final gels	719:761	The ratios of the three components have a remarkable effect on the gelation time and the mechanical properties of the final gels.
24647364	4	9	theme	gels	758:761	arg1	time					710:713	the gelation time	697:713	the gelation time	697:713	The ratios of the three components have a remarkable effect on the gelation time and the mechanical properties of the final gels.
24647364	2	10	theme	oxide	301:305	arg1	components					275:284	three components	269:284	three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	269:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	10	theme	oxide	301:305	arg1	polymer					349:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	11	theme	PEO-b-PVA	330:338	arg1	components					275:284	three components	269:284	three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	269:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	11	theme	PEO-b-PVA	330:338	arg1	polymer					349:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	6	12	theme	gels	1097:1100	arg1	compositions					1077:1088	the compositions	1073:1088	the compositions of the gels	1073:1100	The sugar-responsiveness of the hydrogels was examined by protein loading and release experiments, and the results indicate that this property is also dependent on the compositions of the gels; at a proper component ratio, a new glucose-responsive hydrogel system operating at physiological pH can be obtained.
24647364	2	13	theme	ethylene	292:299	arg1	oxide					301:305	ethylene oxide	292:305	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	7	14	theme	tunable	1328:1334	arg1	glucose-responsiveness					1336:1357	tunable glucose-responsiveness	1328:1357	tunable glucose-responsiveness	1328:1357	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	2	15	theme	acid	398:401	arg1	components					275:284	three components	269:284	three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	269:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	15	theme	acid	398:401	arg1	crosslinker					424:434	phenylboronic acid (PBA)-terminated PEO crosslinker	384:434	phenylboronic acid (PBA)-terminated PEO crosslinker	384:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	16	dep	components	275:284	arg1	α-cyclodextrin					358:371	α-cyclodextrin	358:371	α-cyclodextrin (α-CD)	358:378	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	16	dep	components	275:284	arg1	polymer					349:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	16	dep	components	275:284	arg1	components					275:284	three components	269:284	three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	269:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	16	dep	components	275:284	arg1	crosslinker					424:434	phenylboronic acid (PBA)-terminated PEO crosslinker	384:434	phenylboronic acid (PBA)-terminated PEO crosslinker	384:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	16	dep	components	275:284	arg1	α-CD					374:377	α-CD	374:377	α-CD	374:377	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	7	17	theme	pharmaceutical	1464:1477	arg1	fields					1479:1484	biomedical and pharmaceutical fields	1449:1484	biomedical and pharmaceutical fields	1449:1484	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	2	18	theme	phenylboronic	384:396	arg1	PBA					404:406	PBA	404:406	PBA	404:406	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	18	theme	phenylboronic	384:396	arg1	acid					398:401	phenylboronic acid	384:401	phenylboronic acid (PBA)-terminated PEO crosslinker	384:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	19	theme	alcohol	321:327	arg1	components					275:284	three components	269:284	three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	269:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	19	theme	alcohol	321:327	arg1	polymer					349:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	1	20	theme	novel	95:99	arg1	system					129:134	A novel glucose-responsive hydrogel system	93:134	A novel glucose-responsive hydrogel system based on dynamic covalent chemistry and inclusion complexation	93:197	A novel glucose-responsive hydrogel system based on dynamic covalent chemistry and inclusion complexation was described.
24647364	7	21	from	applications	1433:1444	arg1	fields					1479:1484	biomedical and pharmaceutical fields	1449:1484	biomedical and pharmaceutical fields	1449:1484	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	4	22	theme	remarkable	676:685	arg1	effect					687:692	a remarkable effect	674:692	a remarkable effect	674:692	The ratios of the three components have a remarkable effect on the gelation time and the mechanical properties of the final gels.
24647364	0	23	theme	Glucose-responsive	0:17	arg1	hydrogels					19:27	Glucose-responsive hydrogels	0:27	Glucose-responsive hydrogels	0:27	Glucose-responsive hydrogels based on dynamic covalent chemistry and inclusion complexation.
24647364	5	24	theme	structural	865:874	arg1	ability					885:891	good structural recovery ability	860:891	good structural recovery ability	860:891	In rheological measurements, the hydrogels are demonstrated to possess solid-like behaviour and good structural recovery ability after yielding.
24647364	1	25	theme	glucose-responsive	101:118	arg1	system					129:134	A novel glucose-responsive hydrogel system	93:134	A novel glucose-responsive hydrogel system based on dynamic covalent chemistry and inclusion complexation	93:197	A novel glucose-responsive hydrogel system based on dynamic covalent chemistry and inclusion complexation was described.
24647364	3	26	theme	hydrogel	614:621	arg1	stability					623:631	hydrogel stability	614:631	hydrogel stability	614:631	Dynamic covalent bonds between PVA and PBA provide sugar-responsive crosslinking, and the inclusion complexation between PEO and α-CD can promote hydrogel formation and enhance hydrogel stability.
24647364	7	27	theme	systems	1404:1410	arg1	design					1385:1390	an alternative design	1370:1390	an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes	1370:1515	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	2	28	theme	vinyl	315:319	arg1	components					275:284	three components	269:284	three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	269:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	28	theme	vinyl	315:319	arg1	polymer					349:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	29	theme	-terminated	408:418	arg1	components					275:284	three components	269:284	three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	269:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	29	theme	-terminated	408:418	arg1	crosslinker					424:434	phenylboronic acid (PBA)-terminated PEO crosslinker	384:434	phenylboronic acid (PBA)-terminated PEO crosslinker	384:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	30	theme	PEO	420:422	arg1	components					275:284	three components	269:284	three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	269:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	30	theme	PEO	420:422	arg1	crosslinker					424:434	phenylboronic acid (PBA)-terminated PEO crosslinker	384:434	phenylboronic acid (PBA)-terminated PEO crosslinker	384:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	6	31	theme	release	987:993	arg1	experiments					995:1005	release experiments	987:1005	release experiments	987:1005	The sugar-responsiveness of the hydrogels was examined by protein loading and release experiments, and the results indicate that this property is also dependent on the compositions of the gels; at a proper component ratio, a new glucose-responsive hydrogel system operating at physiological pH can be obtained.
24647364	3	32	theme	inclusion	527:535	arg1	complexation					537:548	the inclusion complexation	523:548	the inclusion complexation between PEO and α-CD	523:569	Dynamic covalent bonds between PVA and PBA provide sugar-responsive crosslinking, and the inclusion complexation between PEO and α-CD can promote hydrogel formation and enhance hydrogel stability.
24647364	5	33	contain	possess	827:833	arg1	hydrogels					797:805	the hydrogels	793:805	the hydrogels	793:805	In rheological measurements, the hydrogels are demonstrated to possess solid-like behaviour and good structural recovery ability after yielding.
24647364	5	33	contain	possess	827:833	arg2	ability					885:891	good structural recovery ability	860:891	good structural recovery ability	860:891	In rheological measurements, the hydrogels are demonstrated to possess solid-like behaviour and good structural recovery ability after yielding.
24647364	5	33	contain	possess	827:833	arg2	behaviour					846:854	solid-like behaviour	835:854	solid-like behaviour	835:854	In rheological measurements, the hydrogels are demonstrated to possess solid-like behaviour and good structural recovery ability after yielding.
24647364	2	34	theme	-b-poly	307:313	arg1	components					275:284	three components	269:284	three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	269:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	34	theme	-b-poly	307:313	arg1	polymer					349:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	4	35	theme	components	658:667	arg1	ratios					638:643	The ratios	634:643	The ratios of the three components	634:667	The ratios of the three components have a remarkable effect on the gelation time and the mechanical properties of the final gels.
24647364	5	36	theme	recovery	876:883	arg1	ability					885:891	good structural recovery ability	860:891	good structural recovery ability	860:891	In rheological measurements, the hydrogels are demonstrated to possess solid-like behaviour and good structural recovery ability after yielding.
24647364	0	37	theme	covalent	46:53	arg1	chemistry					55:63	dynamic covalent chemistry	38:63	dynamic covalent chemistry	38:63	Glucose-responsive hydrogels based on dynamic covalent chemistry and inclusion complexation.
24647364	3	38	theme	sugar-responsive	488:503	arg1	crosslinking					505:516	sugar-responsive crosslinking	488:516	sugar-responsive crosslinking	488:516	Dynamic covalent bonds between PVA and PBA provide sugar-responsive crosslinking, and the inclusion complexation between PEO and α-CD can promote hydrogel formation and enhance hydrogel stability.
24647364	7	39	with	hydrogels	1313:1321	arg1	glucose-responsiveness					1336:1357	tunable glucose-responsiveness	1328:1357	tunable glucose-responsiveness	1328:1357	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	7	40	theme	preparation	1298:1308	arg1	combination					1224:1234	The combination	1220:1234	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness	1220:1357	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	3	41	theme	covalent	445:452	arg1	bonds					454:458	Dynamic covalent bonds	437:458	Dynamic covalent bonds between PVA and PBA	437:478	Dynamic covalent bonds between PVA and PBA provide sugar-responsive crosslinking, and the inclusion complexation between PEO and α-CD can promote hydrogel formation and enhance hydrogel stability.
24647364	0	42	theme	dynamic	38:44	arg1	chemistry					55:63	dynamic covalent chemistry	38:63	dynamic covalent chemistry	38:63	Glucose-responsive hydrogels based on dynamic covalent chemistry and inclusion complexation.
24647364	7	43	theme	potential	1423:1431	arg1	treatment					1495:1503	treatment	1495:1503	treatment of diabetes	1495:1515	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	7	43	theme	potential	1423:1431	arg1	applications					1433:1444	potential applications	1423:1444	potential applications	1423:1444	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	5	44	theme	good	860:863	arg1	ability					885:891	good structural recovery ability	860:891	good structural recovery ability	860:891	In rheological measurements, the hydrogels are demonstrated to possess solid-like behaviour and good structural recovery ability after yielding.
24647364	4	45	theme	final	752:756	arg1	gels					758:761	the final gels	748:761	the final gels	748:761	The ratios of the three components have a remarkable effect on the gelation time and the mechanical properties of the final gels.
24647364	6	46	theme	protein	967:973	arg1	loading					975:981	protein loading	967:981	protein loading	967:981	The sugar-responsiveness of the hydrogels was examined by protein loading and release experiments, and the results indicate that this property is also dependent on the compositions of the gels; at a proper component ratio, a new glucose-responsive hydrogel system operating at physiological pH can be obtained.
24647364	7	47	theme	good	1239:1242	arg1	biocompatibility					1244:1259	good biocompatibility	1239:1259	good biocompatibility of the three components	1239:1283	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	3	48	theme	hydrogel	583:590	arg1	formation					592:600	hydrogel formation	583:600	hydrogel formation	583:600	Dynamic covalent bonds between PVA and PBA provide sugar-responsive crosslinking, and the inclusion complexation between PEO and α-CD can promote hydrogel formation and enhance hydrogel stability.
24647364	7	49	theme	hydrogels	1313:1321	arg1	biocompatibility					1244:1259	good biocompatibility	1239:1259	good biocompatibility of the three components	1239:1283	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	7	49	theme	hydrogels	1313:1321	arg1	preparation					1298:1308	the easy preparation	1289:1308	the easy preparation of hydrogels with tunable glucose-responsiveness	1289:1357	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	6	50	theme	physiological	1186:1198	arg1	pH					1200:1201	physiological pH	1186:1201	physiological pH	1186:1201	The sugar-responsiveness of the hydrogels was examined by protein loading and release experiments, and the results indicate that this property is also dependent on the compositions of the gels; at a proper component ratio, a new glucose-responsive hydrogel system operating at physiological pH can be obtained.
24647364	6	51	theme	glucose-responsive	1138:1155	arg1	system					1166:1171	a new glucose-responsive hydrogel system	1132:1171	a new glucose-responsive hydrogel system operating at physiological pH	1132:1201	The sugar-responsiveness of the hydrogels was examined by protein loading and release experiments, and the results indicate that this property is also dependent on the compositions of the gels; at a proper component ratio, a new glucose-responsive hydrogel system operating at physiological pH can be obtained.
24647364	2	52	theme	diblock	341:347	arg1	components					275:284	three components	269:284	three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	269:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	52	theme	diblock	341:347	arg1	polymer					349:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	7	53	theme	alternative	1373:1383	arg1	design					1385:1390	an alternative design	1370:1390	an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes	1370:1515	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	2	54	theme	poly	287:290	arg1	components					275:284	three components	269:284	three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	269:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	2	54	theme	poly	287:290	arg1	polymer					349:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer	287:355	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	1	55	theme	dynamic	145:151	arg1	chemistry					162:170	dynamic covalent chemistry	145:170	dynamic covalent chemistry	145:170	A novel glucose-responsive hydrogel system based on dynamic covalent chemistry and inclusion complexation was described.
24647364	1	56	theme	hydrogel	120:127	arg1	system					129:134	A novel glucose-responsive hydrogel system	93:134	A novel glucose-responsive hydrogel system based on dynamic covalent chemistry and inclusion complexation	93:197	A novel glucose-responsive hydrogel system based on dynamic covalent chemistry and inclusion complexation was described.
24647364	7	57	theme	biomedical	1449:1458	arg1	fields					1479:1484	biomedical and pharmaceutical fields	1449:1484	biomedical and pharmaceutical fields	1449:1484	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	7	58	theme	easy	1293:1296	arg1	preparation					1298:1308	the easy preparation	1289:1308	the easy preparation of hydrogels with tunable glucose-responsiveness	1289:1357	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24647364	1	59	theme	covalent	153:160	arg1	chemistry					162:170	dynamic covalent chemistry	145:170	dynamic covalent chemistry	145:170	A novel glucose-responsive hydrogel system based on dynamic covalent chemistry and inclusion complexation was described.
24647364	4	60	theme	mechanical	723:732	arg1	properties					734:743	the mechanical properties	719:743	the mechanical properties of the final gels	719:761	The ratios of the three components have a remarkable effect on the gelation time and the mechanical properties of the final gels.
24647364	4	61	contain	have	669:672	arg2	effect					687:692	a remarkable effect	674:692	a remarkable effect	674:692	The ratios of the three components have a remarkable effect on the gelation time and the mechanical properties of the final gels.
24647364	4	61	contain	have	669:672	arg1	ratios					638:643	The ratios	634:643	The ratios of the three components	634:667	The ratios of the three components have a remarkable effect on the gelation time and the mechanical properties of the final gels.
24647364	2	62	theme	components	275:284	arg1	solutions					256:264	the solutions	252:264	the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker	252:434	Hydrogels are formed by simply mixing the solutions of three components: poly(ethylene oxide)-b-poly vinyl alcohol (PEO-b-PVA) diblock polymer, α-cyclodextrin (α-CD) and phenylboronic acid (PBA)-terminated PEO crosslinker.
24647364	5	63	theme	solid-like	835:844	arg1	behaviour					846:854	solid-like behaviour	835:854	solid-like behaviour	835:854	In rheological measurements, the hydrogels are demonstrated to possess solid-like behaviour and good structural recovery ability after yielding.
24647364	6	64	theme	proper	1108:1113	arg1	ratio					1125:1129	a proper component ratio	1106:1129	a proper component ratio	1106:1129	The sugar-responsiveness of the hydrogels was examined by protein loading and release experiments, and the results indicate that this property is also dependent on the compositions of the gels; at a proper component ratio, a new glucose-responsive hydrogel system operating at physiological pH can be obtained.
24647364	3	65	theme	Dynamic	437:443	arg1	bonds					454:458	Dynamic covalent bonds	437:458	Dynamic covalent bonds between PVA and PBA	437:478	Dynamic covalent bonds between PVA and PBA provide sugar-responsive crosslinking, and the inclusion complexation between PEO and α-CD can promote hydrogel formation and enhance hydrogel stability.
24647364	0	66	theme	inclusion	69:77	arg1	complexation					79:90	inclusion complexation	69:90	inclusion complexation	69:90	Glucose-responsive hydrogels based on dynamic covalent chemistry and inclusion complexation.
24647364	6	67	theme	component	1115:1123	arg1	ratio					1125:1129	a proper component ratio	1106:1129	a proper component ratio	1106:1129	The sugar-responsiveness of the hydrogels was examined by protein loading and release experiments, and the results indicate that this property is also dependent on the compositions of the gels; at a proper component ratio, a new glucose-responsive hydrogel system operating at physiological pH can be obtained.
24647364	7	68	theme	biocompatibility	1244:1259	arg1	combination					1224:1234	The combination	1220:1234	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness	1220:1357	The combination of good biocompatibility of the three components and the easy preparation of hydrogels with tunable glucose-responsiveness may enable an alternative design of hydrogel systems that finds potential applications in biomedical and pharmaceutical fields, such as treatment of diabetes.
24764143	10	0	theme	cancer	1236:1241	arg1	origin					1243:1248	fetal and cancer origin	1226:1248	origin	1243:1248	The differences in ligand properties between CA-125 of fetal and cancer origin may be due to specificities of glycosylation.
24764143	5	1	theme	bacterial	624:632	arg1	glycans					634:640	viral and bacterial glycans	614:640	viral and bacterial glycans	614:640	It plays a role as a pattern recognition molecule for viral and bacterial glycans or as an adhesion receptor.
24764143	4	2	gly	mannosylated	519:530	arg1	structures					548:557	mannosylated and fucosylated structures	519:557	structures	548:557	In this study, we assessed CA-125 as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN), which is a C-type lectin showing specificity for mannosylated and fucosylated structures.
24764143	7	3	theme	fetal	855:859	arg1	origin					861:866	fetal origin	855:866	fetal origin	855:866	The results showed that DC-SIGN binds to CA-125 of fetal origin and that this interaction is carbohydrate-dependent.
24764143	10	4	theme	fetal	1226:1230	arg1	origin					1243:1248	fetal and cancer origin	1226:1248	origin	1243:1248	The differences in ligand properties between CA-125 of fetal and cancer origin may be due to specificities of glycosylation.
24764143	3	5	theme	biological	315:324	arg1	roles					326:330	biological roles	315:330	biological roles	315:330	Due to structural heterogeneity, CA-125 ligand capacity and biological roles are not yet understood.
24764143	6	6	theme	inhibition	785:794	arg1	assays					796:801	solid- or fluid-phase binding and inhibition assays	751:801	assays	796:801	We probed a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin using solid- or fluid-phase binding and inhibition assays.
24764143	1	7	theme	extracellular	156:168	arg1	portion					170:176	the extracellular portion	152:176	the extracellular portion of transmembrane mucin 16 (MUC16)	152:210	CA-125 (coelomic epithelium-related antigen) forms the extracellular portion of transmembrane mucin 16 (MUC16).
24764143	6	8	with	chimera	699:705	arg1	CA-125					712:717	CA-125	712:717	CA-125 of fetal or cancer origin	712:743	We probed a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin using solid- or fluid-phase binding and inhibition assays.
24764143	4	9	theme	C-type	481:486	arg1	non-integrin					446:457	non-integrin	446:457	non-integrin	446:457	In this study, we assessed CA-125 as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN), which is a C-type lectin showing specificity for mannosylated and fucosylated structures.
24764143	4	9	theme	C-type	481:486	arg1	lectin					488:493	a C-type lectin	479:493	a C-type lectin showing specificity for mannosylated and fucosylated structures	479:557	In this study, we assessed CA-125 as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN), which is a C-type lectin showing specificity for mannosylated and fucosylated structures.
24764143	9	10	theme	CA-125	1044:1049	arg1	potency					1033:1039	the potency	1029:1039	the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens	1029:1168	Inhibition assays indicated differences in the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens.
24764143	6	11	theme	DC-SIGN-Fc	688:697	arg1	chimera					699:705	a human DC-SIGN-Fc chimera	680:705	a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin	680:743	We probed a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin using solid- or fluid-phase binding and inhibition assays.
24764143	9	12	theme	mannan	1130:1135	arg1	antigens					1161:1168	mannan and Helicobacter pylori antigens	1130:1168	mannan and Helicobacter pylori antigens	1130:1168	Inhibition assays indicated differences in the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens.
24764143	5	13	theme	pattern	581:587	arg1	molecule					601:608	a pattern recognition molecule	579:608	a pattern recognition molecule for viral and bacterial glycans or as an adhesion receptor	579:667	It plays a role as a pattern recognition molecule for viral and bacterial glycans or as an adhesion receptor.
24764143	5	13	theme	pattern	581:587	arg1	role					571:574	a role	569:574	a role	569:574	It plays a role as a pattern recognition molecule for viral and bacterial glycans or as an adhesion receptor.
24764143	6	14	theme	human	682:686	arg1	chimera					699:705	a human DC-SIGN-Fc chimera	680:705	a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin	680:743	We probed a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin using solid- or fluid-phase binding and inhibition assays.
24764143	11	15	theme	various	1317:1323	arg1	functions					1325:1333	various functions	1317:1333	various functions of dendritic cells based on their subset diversity and maturation-related functional capacity	1317:1427	This might influence various functions of dendritic cells based on their subset diversity and maturation-related functional capacity.
24764143	5	16	theme	recognition	589:599	arg1	molecule					601:608	a pattern recognition molecule	579:608	a pattern recognition molecule for viral and bacterial glycans or as an adhesion receptor	579:667	It plays a role as a pattern recognition molecule for viral and bacterial glycans or as an adhesion receptor.
24764143	5	16	theme	recognition	589:599	arg1	role					571:574	a role	569:574	a role	569:574	It plays a role as a pattern recognition molecule for viral and bacterial glycans or as an adhesion receptor.
24764143	7	17	theme	origin	861:866	arg1	CA-125					845:850	CA-125	845:850	CA-125 of fetal origin	845:866	The results showed that DC-SIGN binds to CA-125 of fetal origin and that this interaction is carbohydrate-dependent.
24764143	10	18	theme	glycosylation	1281:1293	arg1	specificities					1264:1276	specificities	1264:1276	specificities of glycosylation	1264:1293	The differences in ligand properties between CA-125 of fetal and cancer origin may be due to specificities of glycosylation.
24764143	11	19	theme	cells	1348:1352	arg1	functions					1325:1333	various functions	1317:1333	various functions of dendritic cells based on their subset diversity and maturation-related functional capacity	1317:1427	This might influence various functions of dendritic cells based on their subset diversity and maturation-related functional capacity.
24764143	11	20	theme	maturation-related	1390:1407	arg1	capacity					1420:1427	maturation-related functional capacity	1390:1427	maturation-related functional capacity	1390:1427	This might influence various functions of dendritic cells based on their subset diversity and maturation-related functional capacity.
24764143	5	21	theme	viral	614:618	arg1	glycans					634:640	viral and bacterial glycans	614:640	viral and bacterial glycans	614:640	It plays a role as a pattern recognition molecule for viral and bacterial glycans or as an adhesion receptor.
24764143	6	22	theme	solid-	751:756	arg1	binding					773:779	solid- or fluid-phase binding and inhibition assays	751:801	binding	773:779	We probed a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin using solid- or fluid-phase binding and inhibition assays.
24764143	1	23	theme	transmembrane	181:193	arg1	mucin					195:199	transmembrane mucin 16	181:202	transmembrane mucin 16 (MUC16)	181:210	CA-125 (coelomic epithelium-related antigen) forms the extracellular portion of transmembrane mucin 16 (MUC16).
24764143	1	23	theme	transmembrane	181:193	arg1	MUC16					205:209	MUC16	205:209	MUC16	205:209	CA-125 (coelomic epithelium-related antigen) forms the extracellular portion of transmembrane mucin 16 (MUC16).
24764143	4	24	theme	fucosylated	536:546	arg1	structures					548:557	mannosylated and fucosylated structures	519:557	structures	548:557	In this study, we assessed CA-125 as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN), which is a C-type lectin showing specificity for mannosylated and fucosylated structures.
24764143	9	25	theme	pathogen-related	1088:1103	arg1	glycoconjugates					1105:1119	pathogen-related glycoconjugates	1088:1119	pathogen-related glycoconjugates	1088:1119	Inhibition assays indicated differences in the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens.
24764143	9	25	theme	pathogen-related	1088:1103	arg1	antigens					1161:1168	mannan and Helicobacter pylori antigens	1130:1168	mannan and Helicobacter pylori antigens	1130:1168	Inhibition assays indicated differences in the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens.
24764143	1	26	theme	mucin	195:199	arg1	portion					170:176	the extracellular portion	152:176	the extracellular portion of transmembrane mucin 16 (MUC16)	152:210	CA-125 (coelomic epithelium-related antigen) forms the extracellular portion of transmembrane mucin 16 (MUC16).
24764143	0	27	theme	origin	16:21	arg1	CA-125					0:5	CA-125	0:5	CA-125 of fetal origin	0:21	CA-125 of fetal origin can act as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin.
24764143	0	27	theme	origin	16:21	arg1	ligand					36:41	a ligand	34:41	a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin	34:98	CA-125 of fetal origin can act as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin.
24764143	3	28	theme	structural	262:271	arg1	heterogeneity					273:285	structural heterogeneity	262:285	structural heterogeneity	262:285	Due to structural heterogeneity, CA-125 ligand capacity and biological roles are not yet understood.
24764143	6	29	theme	origin	738:743	arg1	CA-125					712:717	CA-125	712:717	CA-125 of fetal or cancer origin	712:743	We probed a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin using solid- or fluid-phase binding and inhibition assays.
24764143	9	30	from	differences	1014:1024	arg1	potency					1033:1039	the potency	1029:1039	the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens	1029:1168	Inhibition assays indicated differences in the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens.
24764143	0	31	theme	fetal	10:14	arg1	origin					16:21	fetal origin	10:21	fetal origin	10:21	CA-125 of fetal origin can act as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin.
24764143	10	32	from	differences	1175:1185	arg1	properties					1197:1206	ligand properties	1190:1206	ligand properties	1190:1206	The differences in ligand properties between CA-125 of fetal and cancer origin may be due to specificities of glycosylation.
24764143	11	33	theme	subset	1369:1374	arg1	diversity					1376:1384	their subset diversity	1363:1384	their subset diversity	1363:1384	This might influence various functions of dendritic cells based on their subset diversity and maturation-related functional capacity.
24764143	9	34	theme	DC-SIGN	1069:1075	arg1	binding					1077:1083	DC-SIGN binding	1069:1083	DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens	1069:1168	Inhibition assays indicated differences in the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens.
24764143	9	35	theme	Inhibition	986:995	arg1	assays					997:1002	Inhibition assays	986:1002	Inhibition assays	986:1002	Inhibition assays indicated differences in the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens.
24764143	6	36	theme	cancer	731:736	arg1	origin					738:743	fetal or cancer origin	722:743	origin	738:743	We probed a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin using solid- or fluid-phase binding and inhibition assays.
24764143	8	37	link	cancer-derived	934:947	arg1	CA-125					949:954	cancer-derived CA-125	934:954	cancer-derived CA-125	934:954	By contrast, cancer-derived CA-125 displayed negligible binding.
24764143	3	38	theme	ligand	295:300	arg1	capacity					302:309	CA-125 ligand capacity	288:309	CA-125 ligand capacity	288:309	Due to structural heterogeneity, CA-125 ligand capacity and biological roles are not yet understood.
24764143	4	39	gly	fucosylated	536:546	arg1	structures					548:557	mannosylated and fucosylated structures	519:557	structures	548:557	In this study, we assessed CA-125 as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN), which is a C-type lectin showing specificity for mannosylated and fucosylated structures.
24764143	4	40	theme	mannosylated	519:530	arg1	structures					548:557	mannosylated and fucosylated structures	519:557	structures	548:557	In this study, we assessed CA-125 as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN), which is a C-type lectin showing specificity for mannosylated and fucosylated structures.
24764143	5	41	theme	adhesion	651:658	arg1	receptor					660:667	an adhesion receptor	648:667	an adhesion receptor	648:667	It plays a role as a pattern recognition molecule for viral and bacterial glycans or as an adhesion receptor.
24764143	6	42	theme	fetal	722:726	arg1	origin					738:743	fetal or cancer origin	722:743	origin	738:743	We probed a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin using solid- or fluid-phase binding and inhibition assays.
24764143	1	43	theme	coelomic	109:116	arg1	antigen					137:143	coelomic epithelium-related antigen	109:143	coelomic epithelium-related antigen	109:143	CA-125 (coelomic epithelium-related antigen) forms the extracellular portion of transmembrane mucin 16 (MUC16).
24764143	1	43	theme	coelomic	109:116	arg1	CA-125					101:106	CA-125	101:106	CA-125 (coelomic epithelium-related antigen)	101:144	CA-125 (coelomic epithelium-related antigen) forms the extracellular portion of transmembrane mucin 16 (MUC16).
24764143	9	44	theme	Helicobacter	1141:1152	arg1	pylori					1154:1159	Helicobacter pylori	1141:1159	Helicobacter pylori	1141:1159	Inhibition assays indicated differences in the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens.
24764143	1	45	theme	epithelium-related	118:135	arg1	antigen					137:143	coelomic epithelium-related antigen	109:143	coelomic epithelium-related antigen	109:143	CA-125 (coelomic epithelium-related antigen) forms the extracellular portion of transmembrane mucin 16 (MUC16).
24764143	1	45	theme	epithelium-related	118:135	arg1	CA-125					101:106	CA-125	101:106	CA-125 (coelomic epithelium-related antigen)	101:144	CA-125 (coelomic epithelium-related antigen) forms the extracellular portion of transmembrane mucin 16 (MUC16).
24764143	10	46	theme	origin	1243:1248	arg1	CA-125					1216:1221	CA-125	1216:1221	CA-125 of fetal and cancer origin	1216:1248	The differences in ligand properties between CA-125 of fetal and cancer origin may be due to specificities of glycosylation.
24764143	3	47	theme	CA-125	288:293	arg1	capacity					302:309	CA-125 ligand capacity	288:309	CA-125 ligand capacity	288:309	Due to structural heterogeneity, CA-125 ligand capacity and biological roles are not yet understood.
24764143	9	48	theme	pylori	1154:1159	arg1	antigens					1161:1168	mannan and Helicobacter pylori antigens	1130:1168	mannan and Helicobacter pylori antigens	1130:1168	Inhibition assays indicated differences in the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens.
24764143	6	49	theme	fluid-phase	761:771	arg1	binding					773:779	solid- or fluid-phase binding and inhibition assays	751:801	binding	773:779	We probed a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin using solid- or fluid-phase binding and inhibition assays.
24764143	10	50	theme	ligand	1190:1195	arg1	properties					1197:1206	ligand properties	1190:1206	ligand properties	1190:1206	The differences in ligand properties between CA-125 of fetal and cancer origin may be due to specificities of glycosylation.
24764143	11	51	theme	functional	1409:1418	arg1	capacity					1420:1427	maturation-related functional capacity	1390:1427	maturation-related functional capacity	1390:1427	This might influence various functions of dendritic cells based on their subset diversity and maturation-related functional capacity.
24764143	2	52	theme	proteolytic	230:240	arg1	degradation					242:252	proteolytic degradation	230:252	proteolytic degradation	230:252	It is shed after proteolytic degradation.
24764143	8	53	theme	negligible	966:975	arg1	binding					977:983	negligible binding	966:983	negligible binding	966:983	By contrast, cancer-derived CA-125 displayed negligible binding.
24764143	11	54	theme	dendritic	1338:1346	arg1	cells					1348:1352	dendritic cells	1338:1352	dendritic cells based on their subset diversity and maturation-related functional capacity	1338:1427	This might influence various functions of dendritic cells based on their subset diversity and maturation-related functional capacity.
24764143	8	55	theme	cancer-derived	934:947	arg1	CA-125					949:954	cancer-derived CA-125	934:954	cancer-derived CA-125	934:954	By contrast, cancer-derived CA-125 displayed negligible binding.
27102717	8	0	theme	fruit	952:956	arg1	juice					958:962	particular fruit juice	941:962	particular fruit juice	941:962	Intakes of total fruits, in particular fruit juice, increased with age.
27102717	2	1	theme	Irish	518:522	arg1	children					524:531	Irish children	518:531	Irish children aged 1-4 years	518:546	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	11	2	theme	%	1335:1335	arg1	%					1329:1329	20 %	1326:1329	20 % (15 % fruit; 5 % vegetables)	1326:1358	F&V contributed on average 20 % (15 % fruit; 5 % vegetables) to the weight of the total diet and was <10 % in sixty-one children (12 %).
27102717	11	2	theme	%	1335:1335	arg1	%					1346:1346	15 % fruit; 5 % vegetables	1332:1357	15 % fruit; 5 % vegetables	1332:1357	F&V contributed on average 20 % (15 % fruit; 5 % vegetables) to the weight of the total diet and was <10 % in sixty-one children (12 %).
27102717	0	3	theme	Nutrition	134:142	arg1	Survey					144:149	the Irish National Pre-School Nutrition Survey	104:149	the Irish National Pre-School Nutrition Survey	104:149	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	1	4	theme	intakes	205:211	arg1	importance					165:174	the importance	161:174	the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases	161:249	Although the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases is well established, there are limited data on intakes in very young children.
27102717	9	5	from	fruits	1037:1042	arg1	dishes					1153:1158	composite dishes	1143:1158	composite dishes (7-13 %)	1143:1167	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	9	5	from	fruits	1037:1042	arg1	%					1166:1166	7-13 %	1161:1166	7-13 %	1161:1166	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	10	6	theme	composite	1271:1279	arg1	dishes					1281:1286	composite dishes	1271:1286	composite dishes	1271:1286	Total vegetable intake comprised of discrete vegetables (48-62 % range across age) and vegetables in composite dishes (38-52 %).
27102717	11	7	theme	fruit	1337:1341	arg1	%					1329:1329	20 %	1326:1329	20 % (15 % fruit; 5 % vegetables)	1326:1358	F&V contributed on average 20 % (15 % fruit; 5 % vegetables) to the weight of the total diet and was <10 % in sixty-one children (12 %).
27102717	11	7	theme	fruit	1337:1341	arg1	%					1346:1346	15 % fruit; 5 % vegetables	1332:1357	15 % fruit; 5 % vegetables	1332:1357	F&V contributed on average 20 % (15 % fruit; 5 % vegetables) to the weight of the total diet and was <10 % in sixty-one children (12 %).
27102717	5	8	theme	edible	697:702	arg1	fruits					704:709	edible fruits	697:709	edible fruits	697:709	The percentage of edible fruits and/or vegetables in each food code was calculated.
27102717	9	9	from	juice	1084:1088	arg1	dishes					1153:1158	composite dishes	1143:1158	composite dishes (7-13 %)	1143:1167	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	9	9	from	juice	1084:1088	arg1	%					1166:1166	7-13 %	1161:1166	7-13 %	1161:1166	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	2	10	theme	aged	533:536	arg1	children					524:531	Irish children	518:531	Irish children aged 1-4 years	518:546	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	9	11	theme	fruit	1011:1015	arg1	intake					1017:1022	total fruit intake	1005:1022	total fruit intake	1005:1022	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	9	12	theme	47-56	1045:1049	arg1	%					1051:1051	%	1051:1051	%	1051:1051	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	11	13	from	%	1404:1404	arg1	children					1419:1426	sixty-one children	1409:1426	sixty-one children	1409:1426	F&V contributed on average 20 % (15 % fruit; 5 % vegetables) to the weight of the total diet and was <10 % in sixty-one children (12 %).
27102717	1	14	theme	young	314:318	arg1	children					320:327	very young children	309:327	very young children	309:327	Although the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases is well established, there are limited data on intakes in very young children.
27102717	13	15	theme	patterns	1674:1681	arg1	aspects					1652:1658	some aspects	1647:1658	some aspects of F&V intake patterns	1647:1681	F&V are important components of the diet of Irish pre-school children; however, some aspects of F&V intake patterns could be improved in this age group.
27102717	1	16	theme	fruit	179:183	arg1	intakes					205:211	fruit and vegetable (F&V) intakes	179:211	fruit and vegetable (F&V) intakes	179:211	Although the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases is well established, there are limited data on intakes in very young children.
27102717	1	16	theme	fruit	179:183	arg1	F&V					200:202	F&V	200:202	F&V	200:202	Although the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases is well established, there are limited data on intakes in very young children.
27102717	12	17	theme	non-milk	1523:1530	arg1	intakes					1538:1544	non-milk sugar intakes	1523:1544	non-milk sugar intakes	1523:1544	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	11	18	dep	%	1404:1404	arg1	%					1432:1432	12 %	1429:1432	12 %	1429:1432	F&V contributed on average 20 % (15 % fruit; 5 % vegetables) to the weight of the total diet and was <10 % in sixty-one children (12 %).
27102717	0	19	theme	National	114:121	arg1	Survey					144:149	the Irish National Pre-School Nutrition Survey	104:149	the Irish National Pre-School Nutrition Survey	104:149	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	4	20	dep	food	621:624	arg1	codes					626:630	codes	626:630	codes	626:630	Of 1652 food codes consumed, 740 had a fruit/vegetable component.
27102717	11	21	theme	diet	1387:1390	arg1	weight					1367:1372	the weight	1363:1372	the weight of the total diet	1363:1390	F&V contributed on average 20 % (15 % fruit; 5 % vegetables) to the weight of the total diet and was <10 % in sixty-one children (12 %).
27102717	10	22	dep	%	1233:1233	arg1	range					1235:1239	range	1235:1239	48-62 % range	1227:1239	Total vegetable intake comprised of discrete vegetables (48-62 % range across age) and vegetables in composite dishes (38-52 %).
27102717	13	23	theme	pre-school	1617:1626	arg1	children					1628:1635	Irish pre-school children	1611:1635	Irish pre-school children	1611:1635	F&V are important components of the diet of Irish pre-school children; however, some aspects of F&V intake patterns could be improved in this age group.
27102717	1	24	from	intakes	298:304	arg1	children					320:327	very young children	309:327	very young children	309:327	Although the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases is well established, there are limited data on intakes in very young children.
27102717	6	25	theme	diet	847:850	arg1	weight					827:832	the weight	823:832	the weight of the total diet (%)	823:854	Intakes (g/d), sources (g/d) and the contribution of F&V to the weight of the total diet (%) were estimated, split by age.
27102717	10	26	theme	Total	1170:1174	arg1	intake					1186:1191	Total vegetable intake	1170:1191	Total vegetable intake	1170:1191	Total vegetable intake comprised of discrete vegetables (48-62 % range across age) and vegetables in composite dishes (38-52 %).
27102717	11	27	theme	sixty-one	1409:1417	arg1	children					1419:1426	sixty-one children	1409:1426	sixty-one children	1409:1426	F&V contributed on average 20 % (15 % fruit; 5 % vegetables) to the weight of the total diet and was <10 % in sixty-one children (12 %).
27102717	2	28	theme	Nutrition	454:462	arg1	sample					500:505	a nationally representative sample	472:505	a nationally representative sample (n 500) of Irish children aged 1-4 years	472:546	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	2	28	theme	Nutrition	454:462	arg1	Survey					464:469	the National Pre-School Nutrition Survey	430:469	the National Pre-School Nutrition Survey	430:469	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	9	29	theme	%	1076:1076	arg1	juice					1084:1088	100 % fruit juice	1072:1088	100 % fruit juice	1072:1088	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	12	30	theme	fibre	1505:1509	arg1	%					1455:1455	50 %	1452:1455	50 % of vitamin C	1452:1468	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	30	theme	fibre	1505:1509	arg1	fibre					1505:1509	fibre	1505:1509	fibre	1505:1509	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	30	theme	fibre	1505:1509	arg1	intakes					1538:1544	non-milk sugar intakes	1523:1544	non-milk sugar intakes	1523:1544	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	30	theme	fibre	1505:1509	arg1	carotene					1479:1486	carotene	1479:1486	carotene	1479:1486	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	30	theme	fibre	1505:1509	arg1	C					1468:1468	vitamin C	1460:1468	vitamin C	1460:1468	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	30	theme	fibre	1505:1509	arg1	%					1492:1492	34 %	1489:1492	34 % of dietary fibre	1489:1509	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	30	theme	fibre	1505:1509	arg1	%					1518:1518	42 %	1515:1518	42 % of non-milk sugar intakes	1515:1544	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	30	theme	fibre	1505:1509	arg1	%					1474:1474	53 %	1471:1474	53 % of carotene	1471:1486	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	8	31	from	Intakes	913:919	arg1	juice					958:962	particular fruit juice	941:962	particular fruit juice	941:962	Intakes of total fruits, in particular fruit juice, increased with age.
27102717	2	32	theme	National	434:441	arg1	sample					500:505	a nationally representative sample	472:505	a nationally representative sample (n 500) of Irish children aged 1-4 years	472:546	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	2	32	theme	National	434:441	arg1	Survey					464:469	the National Pre-School Nutrition Survey	430:469	the National Pre-School Nutrition Survey	430:469	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	4	33	theme	fruit/vegetable	652:666	arg1	component					668:676	a fruit/vegetable component	650:676	a fruit/vegetable component	650:676	Of 1652 food codes consumed, 740 had a fruit/vegetable component.
27102717	12	34	theme	carotene	1479:1486	arg1	%					1455:1455	50 %	1452:1455	50 % of vitamin C	1452:1468	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	34	theme	carotene	1479:1486	arg1	fibre					1505:1509	fibre	1505:1509	fibre	1505:1509	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	34	theme	carotene	1479:1486	arg1	intakes					1538:1544	non-milk sugar intakes	1523:1544	non-milk sugar intakes	1523:1544	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	34	theme	carotene	1479:1486	arg1	carotene					1479:1486	carotene	1479:1486	carotene	1479:1486	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	34	theme	carotene	1479:1486	arg1	C					1468:1468	vitamin C	1460:1468	vitamin C	1460:1468	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	34	theme	carotene	1479:1486	arg1	%					1492:1492	34 %	1489:1492	34 % of dietary fibre	1489:1509	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	34	theme	carotene	1479:1486	arg1	%					1518:1518	42 %	1515:1518	42 % of non-milk sugar intakes	1515:1544	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	34	theme	carotene	1479:1486	arg1	%					1474:1474	53 %	1471:1474	53 % of carotene	1471:1486	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	0	35	theme	Fruit	0:4	arg1	intakes					20:26	Fruit and vegetable intakes	0:26	Fruit and vegetable intakes	0:26	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	10	36	dep	comprised	1193:1201	arg1	%					1295:1295	38-52 %	1289:1295	38-52 %	1289:1295	Total vegetable intake comprised of discrete vegetables (48-62 % range across age) and vegetables in composite dishes (38-52 %).
27102717	1	37	theme	chronic	234:240	arg1	diseases					242:249	chronic diseases	234:249	chronic diseases	234:249	Although the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases is well established, there are limited data on intakes in very young children.
27102717	2	38	theme	total	403:407	arg1	diet					409:412	the total diet	399:412	the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years	399:546	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	3	39	theme	weighed	555:561	arg1	record					568:573	A 4-d weighed food record	549:573	A 4-d weighed food record	549:573	A 4-d weighed food record was used to collect food intake data.
27102717	1	40	from	data	290:293	arg1	intakes					298:304	intakes	298:304	intakes in very young children	298:327	Although the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases is well established, there are limited data on intakes in very young children.
27102717	13	41	theme	important	1575:1583	arg1	F&V					1567:1569	F&V	1567:1569	F&V	1567:1569	F&V are important components of the diet of Irish pre-school children; however, some aspects of F&V intake patterns could be improved in this age group.
27102717	13	41	theme	important	1575:1583	arg1	components					1585:1594	important components	1575:1594	important components of the diet of Irish pre-school children	1575:1635	F&V are important components of the diet of Irish pre-school children; however, some aspects of F&V intake patterns could be improved in this age group.
27102717	11	42	theme	total	1381:1385	arg1	diet					1387:1390	the total diet	1377:1390	the total diet	1377:1390	F&V contributed on average 20 % (15 % fruit; 5 % vegetables) to the weight of the total diet and was <10 % in sixty-one children (12 %).
27102717	13	43	theme	age	1709:1711	arg1	group					1713:1717	this age group	1704:1717	this age group	1704:1717	F&V are important components of the diet of Irish pre-school children; however, some aspects of F&V intake patterns could be improved in this age group.
27102717	12	44	theme	vitamin	1460:1466	arg1	C					1468:1468	vitamin C	1460:1468	vitamin C	1460:1468	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	2	45	from	Survey	464:469	arg1	data					420:423	data	420:423	data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years	420:546	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	12	46	theme	intakes	1538:1544	arg1	%					1455:1455	50 %	1452:1455	50 % of vitamin C	1452:1468	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	46	theme	intakes	1538:1544	arg1	fibre					1505:1509	fibre	1505:1509	fibre	1505:1509	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	46	theme	intakes	1538:1544	arg1	intakes					1538:1544	non-milk sugar intakes	1523:1544	non-milk sugar intakes	1523:1544	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	46	theme	intakes	1538:1544	arg1	carotene					1479:1486	carotene	1479:1486	carotene	1479:1486	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	46	theme	intakes	1538:1544	arg1	C					1468:1468	vitamin C	1460:1468	vitamin C	1460:1468	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	46	theme	intakes	1538:1544	arg1	%					1492:1492	34 %	1489:1492	34 % of dietary fibre	1489:1509	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	46	theme	intakes	1538:1544	arg1	%					1518:1518	42 %	1515:1518	42 % of non-milk sugar intakes	1515:1544	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	46	theme	intakes	1538:1544	arg1	%					1474:1474	53 %	1471:1474	53 % of carotene	1471:1486	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	3	47	theme	intake	600:605	arg1	data					607:610	food intake data	595:610	food intake data	595:610	A 4-d weighed food record was used to collect food intake data.
27102717	9	48	theme	composite	1143:1151	arg1	dishes					1153:1158	composite dishes	1143:1158	composite dishes (7-13 %)	1143:1167	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	9	48	theme	composite	1143:1151	arg1	%					1166:1166	7-13 %	1161:1166	7-13 %	1161:1166	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	8	49	theme	particular	941:950	arg1	juice					958:962	particular fruit juice	941:962	particular fruit juice	941:962	Intakes of total fruits, in particular fruit juice, increased with age.
27102717	12	50	theme	total	1555:1559	arg1	diet					1561:1564	the total diet	1551:1564	the total diet	1551:1564	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	9	51	theme	total	1005:1009	arg1	intake					1017:1022	total fruit intake	1005:1022	total fruit intake	1005:1022	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	12	52	theme	sugar	1532:1536	arg1	intakes					1538:1544	non-milk sugar intakes	1523:1544	non-milk sugar intakes	1523:1544	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	2	53	theme	children	524:531	arg1	sample					500:505	a nationally representative sample	472:505	a nationally representative sample (n 500) of Irish children aged 1-4 years	472:546	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	2	53	theme	children	524:531	arg1	Survey					464:469	the National Pre-School Nutrition Survey	430:469	the National Pre-School Nutrition Survey	430:469	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	2	53	theme	children	524:531	arg1	n					508:508	n 500	508:512	n 500	508:512	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	5	54	theme	vegetables	718:727	arg1	percentage					683:692	The percentage	679:692	The percentage of edible fruits and/or vegetables in each food code	679:745	The percentage of edible fruits and/or vegetables in each food code was calculated.
27102717	6	55	theme	F&V	816:818	arg1	g/d					772:774	g/d	772:774	g/d	772:774	Intakes (g/d), sources (g/d) and the contribution of F&V to the weight of the total diet (%) were estimated, split by age.
27102717	6	55	theme	F&V	816:818	arg1	sources					778:784	sources	778:784	sources (g/d)	778:790	Intakes (g/d), sources (g/d) and the contribution of F&V to the weight of the total diet (%) were estimated, split by age.
27102717	6	55	theme	F&V	816:818	arg1	Intakes					763:769	Intakes	763:769	Intakes (g/d)	763:775	Intakes (g/d), sources (g/d) and the contribution of F&V to the weight of the total diet (%) were estimated, split by age.
27102717	6	55	theme	F&V	816:818	arg1	contribution					800:811	the contribution	796:811	the contribution of F&V to the weight of the total diet (%)	796:854	Intakes (g/d), sources (g/d) and the contribution of F&V to the weight of the total diet (%) were estimated, split by age.
27102717	6	55	theme	F&V	816:818	arg1	g/d					787:789	g/d	787:789	g/d	787:789	Intakes (g/d), sources (g/d) and the contribution of F&V to the weight of the total diet (%) were estimated, split by age.
27102717	9	56	theme	discrete	1028:1035	arg1	fruits					1037:1042	discrete fruits	1028:1042	discrete fruits (47-56 % range across age)	1028:1069	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	9	56	theme	discrete	1028:1035	arg1	contribution					989:1000	The contribution	985:1000	The contribution to total fruit intake	985:1022	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	0	57	dep	Survey	144:149	arg1	sources					29:35	sources	29:35	sources	29:35	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	0	57	dep	Survey	144:149	arg1	intakes					20:26	Fruit and vegetable intakes	0:26	Fruit and vegetable intakes	0:26	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	0	57	dep	Survey	144:149	arg1	contribution					41:52	contribution	41:52	contribution to total diet in very young children (1-4 years)	41:101	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	13	58	theme	children	1628:1635	arg1	diet					1603:1606	the diet	1599:1606	the diet of Irish pre-school children	1599:1635	F&V are important components of the diet of Irish pre-school children; however, some aspects of F&V intake patterns could be improved in this age group.
27102717	10	59	dep	vegetables	1215:1224	arg1	%					1233:1233	48-62 %	1227:1233	48-62 % range	1227:1239	Total vegetable intake comprised of discrete vegetables (48-62 % range across age) and vegetables in composite dishes (38-52 %).
27102717	0	60	theme	Irish	108:112	arg1	Survey					144:149	the Irish National Pre-School Nutrition Survey	104:149	the Irish National Pre-School Nutrition Survey	104:149	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	12	61	theme	C	1468:1468	arg1	%					1455:1455	50 %	1452:1455	50 % of vitamin C	1452:1468	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	61	theme	C	1468:1468	arg1	fibre					1505:1509	fibre	1505:1509	fibre	1505:1509	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	61	theme	C	1468:1468	arg1	intakes					1538:1544	non-milk sugar intakes	1523:1544	non-milk sugar intakes	1523:1544	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	61	theme	C	1468:1468	arg1	carotene					1479:1486	carotene	1479:1486	carotene	1479:1486	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	61	theme	C	1468:1468	arg1	C					1468:1468	vitamin C	1460:1468	vitamin C	1460:1468	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	61	theme	C	1468:1468	arg1	%					1492:1492	34 %	1489:1492	34 % of dietary fibre	1489:1509	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	61	theme	C	1468:1468	arg1	%					1518:1518	42 %	1515:1518	42 % of non-milk sugar intakes	1515:1544	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	12	61	theme	C	1468:1468	arg1	%					1474:1474	53 %	1471:1474	53 % of carotene	1471:1486	F&V contributed 50 % of vitamin C, 53 % of carotene, 34 % of dietary fibre and 42 % of non-milk sugar intakes from the total diet.
27102717	4	62	contain	had	646:648	arg2	component					668:676	a fruit/vegetable component	650:676	a fruit/vegetable component	650:676	Of 1652 food codes consumed, 740 had a fruit/vegetable component.
27102717	4	62	contain	had	646:648	arg1	740					642:644	740	642:644	740	642:644	Of 1652 food codes consumed, 740 had a fruit/vegetable component.
27102717	0	63	theme	Pre-School	123:132	arg1	Survey					144:149	the Irish National Pre-School Nutrition Survey	104:149	the Irish National Pre-School Nutrition Survey	104:149	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	9	64	theme	%	1051:1051	arg1	range					1053:1057	47-56 % range	1045:1057	47-56 % range across age	1045:1068	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	1	65	from	importance	165:174	arg1	prevention					220:229	the prevention	216:229	the prevention of chronic diseases	216:249	Although the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases is well established, there are limited data on intakes in very young children.
27102717	2	66	theme	representative	485:498	arg1	sample					500:505	a nationally representative sample	472:505	a nationally representative sample (n 500) of Irish children aged 1-4 years	472:546	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	2	66	theme	representative	485:498	arg1	Survey					464:469	the National Pre-School Nutrition Survey	430:469	the National Pre-School Nutrition Survey	430:469	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	2	66	theme	representative	485:498	arg1	n					508:508	n 500	508:512	n 500	508:512	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	0	67	theme	total	57:61	arg1	diet					63:66	total diet	57:66	total diet	57:66	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	0	68	from	intakes	20:26	arg1	children					82:89	very young children	71:89	very young children (1-4 years)	71:101	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	0	68	from	intakes	20:26	arg1	years					96:100	1-4 years	92:100	1-4 years	92:100	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	5	69	from	percentage	683:692	arg1	food					737:740	food	737:740	food	737:740	The percentage of edible fruits and/or vegetables in each food code was calculated.
27102717	8	70	theme	total	924:928	arg1	fruits					930:935	total fruits	924:935	total fruits	924:935	Intakes of total fruits, in particular fruit juice, increased with age.
27102717	0	71	theme	vegetable	10:18	arg1	intakes					20:26	Fruit and vegetable intakes	0:26	Fruit and vegetable intakes	0:26	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	6	72	theme	total	841:845	arg1	%					853:853	%	853:853	%	853:853	Intakes (g/d), sources (g/d) and the contribution of F&V to the weight of the total diet (%) were estimated, split by age.
27102717	6	72	theme	total	841:845	arg1	diet					847:850	the total diet	837:850	the total diet (%)	837:854	Intakes (g/d), sources (g/d) and the contribution of F&V to the weight of the total diet (%) were estimated, split by age.
27102717	13	73	theme	F&V	1663:1665	arg1	patterns					1674:1681	F&V intake patterns	1663:1681	F&V intake patterns	1663:1681	F&V are important components of the diet of Irish pre-school children; however, some aspects of F&V intake patterns could be improved in this age group.
27102717	0	74	theme	young	76:80	arg1	children					82:89	very young children	71:89	very young children (1-4 years)	71:101	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	0	74	theme	young	76:80	arg1	years					96:100	1-4 years	92:100	1-4 years	92:100	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	9	75	theme	100	1072:1074	arg1	%					1076:1076	%	1076:1076	%	1076:1076	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	2	76	theme	Pre-School	443:452	arg1	sample					500:505	a nationally representative sample	472:505	a nationally representative sample (n 500) of Irish children aged 1-4 years	472:546	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	2	76	theme	Pre-School	443:452	arg1	Survey					464:469	the National Pre-School Nutrition Survey	430:469	the National Pre-School Nutrition Survey	430:469	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	0	77	from	contribution	41:52	arg1	children					82:89	very young children	71:89	very young children (1-4 years)	71:101	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	0	77	from	contribution	41:52	arg1	years					96:100	1-4 years	92:100	1-4 years	92:100	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	9	78	theme	fruit	1078:1082	arg1	juice					1084:1088	100 % fruit juice	1072:1088	100 % fruit juice	1072:1088	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	0	79	from	sources	29:35	arg1	children					82:89	very young children	71:89	very young children (1-4 years)	71:101	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	0	79	from	sources	29:35	arg1	years					96:100	1-4 years	92:100	1-4 years	92:100	Fruit and vegetable intakes, sources and contribution to total diet in very young children (1-4 years): the Irish National Pre-School Nutrition Survey.
27102717	13	80	theme	Irish	1611:1615	arg1	children					1628:1635	Irish pre-school children	1611:1635	Irish pre-school children	1611:1635	F&V are important components of the diet of Irish pre-school children; however, some aspects of F&V intake patterns could be improved in this age group.
27102717	9	81	from	smoothies	1091:1099	arg1	dishes					1153:1158	composite dishes	1143:1158	composite dishes (7-13 %)	1143:1167	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	9	81	from	smoothies	1091:1099	arg1	%					1166:1166	7-13 %	1161:1166	7-13 %	1161:1166	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	13	82	theme	diet	1603:1606	arg1	F&V					1567:1569	F&V	1567:1569	F&V	1567:1569	F&V are important components of the diet of Irish pre-school children; however, some aspects of F&V intake patterns could be improved in this age group.
27102717	13	82	theme	diet	1603:1606	arg1	components					1585:1594	important components	1575:1594	important components of the diet of Irish pre-school children	1575:1635	F&V are important components of the diet of Irish pre-school children; however, some aspects of F&V intake patterns could be improved in this age group.
27102717	10	83	theme	vegetable	1176:1184	arg1	intake					1186:1191	Total vegetable intake	1170:1191	Total vegetable intake	1170:1191	Total vegetable intake comprised of discrete vegetables (48-62 % range across age) and vegetables in composite dishes (38-52 %).
27102717	13	84	theme	intake	1667:1672	arg1	patterns					1674:1681	F&V intake patterns	1663:1681	F&V intake patterns	1663:1681	F&V are important components of the diet of Irish pre-school children; however, some aspects of F&V intake patterns could be improved in this age group.
27102717	5	85	theme	fruits	704:709	arg1	percentage					683:692	The percentage	679:692	The percentage of edible fruits and/or vegetables in each food code	679:745	The percentage of edible fruits and/or vegetables in each food code was calculated.
27102717	3	86	theme	4-d	551:553	arg1	record					568:573	A 4-d weighed food record	549:573	A 4-d weighed food record	549:573	A 4-d weighed food record was used to collect food intake data.
27102717	2	87	theme	F&V	351:353	arg1	intakes					355:361	F&V intakes	351:361	F&V intakes	351:361	This study estimates F&V intakes and sources and the contribution to the total diet using data from the National Pre-School Nutrition Survey, a nationally representative sample (n 500) of Irish children aged 1-4 years.
27102717	1	88	theme	diseases	242:249	arg1	prevention					220:229	the prevention	216:229	the prevention of chronic diseases	216:249	Although the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases is well established, there are limited data on intakes in very young children.
27102717	10	89	theme	discrete	1206:1213	arg1	vegetables					1215:1224	discrete vegetables	1206:1224	discrete vegetables (48-62 % range across age)	1206:1251	Total vegetable intake comprised of discrete vegetables (48-62 % range across age) and vegetables in composite dishes (38-52 %).
27102717	9	90	from	fruits	1133:1138	arg1	dishes					1153:1158	composite dishes	1143:1158	composite dishes (7-13 %)	1143:1167	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	9	90	from	fruits	1133:1138	arg1	%					1166:1166	7-13 %	1161:1166	7-13 %	1161:1166	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	3	91	used	used	579:582	arg2	record					568:573	A 4-d weighed food record	549:573	A 4-d weighed food record	549:573	A 4-d weighed food record was used to collect food intake data.
27102717	11	92	dep	%	1346:1346	arg1	vegetables					1348:1357	vegetables	1348:1357	15 % fruit; 5 % vegetables	1332:1357	F&V contributed on average 20 % (15 % fruit; 5 % vegetables) to the weight of the total diet and was <10 % in sixty-one children (12 %).
27102717	8	93	theme	fruits	930:935	arg1	Intakes					913:919	Intakes	913:919	Intakes	913:919	Intakes of total fruits, in particular fruit juice, increased with age.
27102717	9	94	dep	fruits	1037:1042	arg1	range					1053:1057	47-56 % range	1045:1057	47-56 % range across age	1045:1068	The contribution to total fruit intake was discrete fruits (47-56 % range across age), 100 % fruit juice, smoothies and pureés (32-45 %) as well as fruits in composite dishes (7-13 %).
27102717	3	95	theme	food	563:566	arg1	record					568:573	A 4-d weighed food record	549:573	A 4-d weighed food record	549:573	A 4-d weighed food record was used to collect food intake data.
27102717	5	96	dep	food	737:740	arg1	code					742:745	code	742:745	code	742:745	The percentage of edible fruits and/or vegetables in each food code was calculated.
27102717	1	97	theme	vegetable	189:197	arg1	intakes					205:211	fruit and vegetable (F&V) intakes	179:211	fruit and vegetable (F&V) intakes	179:211	Although the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases is well established, there are limited data on intakes in very young children.
27102717	1	97	theme	vegetable	189:197	arg1	F&V					200:202	F&V	200:202	F&V	200:202	Although the importance of fruit and vegetable (F&V) intakes in the prevention of chronic diseases is well established, there are limited data on intakes in very young children.
27102717	3	98	theme	food	595:598	arg1	data					607:610	food intake data	595:610	food intake data	595:610	A 4-d weighed food record was used to collect food intake data.
26163297	2	0	theme	down	448:451	arg1	version					453:459	a stripped down version	437:459	a stripped down version of the cell-wall peptidoglycan hydrolase MltA	437:505	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	1	1	theme	peptidoglycan	313:325	arg1	domain					336:341	a different putative peptidoglycan cleaving domain	292:341	a different putative peptidoglycan cleaving domain	292:341	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	2	2	theme	stripped	439:446	arg1	version					453:459	a stripped down version	437:459	a stripped down version of the cell-wall peptidoglycan hydrolase MltA	437:505	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	1	3	theme	cleaving	327:334	arg1	domain					336:341	a different putative peptidoglycan cleaving domain	292:341	a different putative peptidoglycan cleaving domain	292:341	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	5	4	from	simplification	872:885	arg1	proteins					902:909	Sps proteins	898:909	Sps proteins	898:909	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	2	5	theme	hydrolase	492:500	arg1	MltA					502:505	the cell-wall peptidoglycan hydrolase MltA	464:505	the cell-wall peptidoglycan hydrolase MltA	464:505	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	2	6	dep	RESULTS	344:350	arg1	found					425:429	found	425:429	is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA	422:505	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	1	7	theme	resuscitation	232:244	arg1	factors					256:262	the resuscitation promoting factors	228:262	the resuscitation promoting factors of Actinobacteria	228:280	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	2	8	theme	peptidoglycan	478:490	arg1	MltA					502:505	the cell-wall peptidoglycan hydrolase MltA	464:505	the cell-wall peptidoglycan hydrolase MltA	464:505	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	5	9	theme	proteins	1077:1084	arg1	Actinobacteria					981:994	Actinobacteria	981:994	Actinobacteria	981:994	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	9	theme	proteins	1077:1084	arg1	versions					1021:1028	stripped down versions	1007:1028	stripped down versions of lysozyme and soluble lytic transglycosylase proteins	1007:1084	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	3	10	theme	YuiC	512:515	arg1	structures					517:526	The YuiC structures	508:526	The YuiC structures	508:526	The YuiC structures are of a domain swapped dimer, although some monomer is also found in solution.
26163297	1	11	theme	promoting	246:254	arg1	factors					256:262	the resuscitation promoting factors	228:262	the resuscitation promoting factors of Actinobacteria	228:280	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	2	12	theme	cell-wall	468:476	arg1	MltA					502:505	the cell-wall peptidoglycan hydrolase MltA	464:505	the cell-wall peptidoglycan hydrolase MltA	464:505	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	1	13	located	found	130:134	arg2	proteins					110:117	BACKGROUND Stationary phase survival proteins	73:117	BACKGROUND Stationary phase survival proteins (Sps)	73:123	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	1	13	located	found	130:134	arg1	Firmicutes					139:148	Firmicutes	139:148	Firmicutes	139:148	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	1	13	located	found	130:134	arg2	Sps					120:122	Sps	120:122	Sps	120:122	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	5	14	theme	resuscitation	939:951	arg1	Actinobacteria					981:994	Actinobacteria	981:994	Actinobacteria	981:994	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	14	theme	resuscitation	939:951	arg1	domains					970:976	the resuscitation promoting factor domains	935:976	the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins	935:1084	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	15	theme	Sps	898:900	arg1	proteins					902:909	Sps proteins	898:909	Sps proteins	898:909	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	2	16	theme	Firmicute	377:385	arg1	YuiC					399:402	a Firmicute Sps protein YuiC	375:402	a Firmicute Sps protein YuiC	375:402	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	1	17	theme	BACKGROUND	73:82	arg1	Sps					120:122	Sps	120:122	Sps	120:122	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	1	17	theme	BACKGROUND	73:82	arg1	proteins					110:117	BACKGROUND Stationary phase survival proteins	73:117	BACKGROUND Stationary phase survival proteins (Sps)	73:123	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	1	18	theme	analogous	160:168	arg1	compositions					177:188	analogous domain compositions	160:188	analogous domain compositions	160:188	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	5	19	theme	lytic	1054:1058	arg1	transglycosylase					1060:1075	soluble lytic transglycosylase	1046:1075	soluble lytic transglycosylase	1046:1075	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	1	20	theme	Stationary	84:93	arg1	Sps					120:122	Sps	120:122	Sps	120:122	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	1	20	theme	Stationary	84:93	arg1	proteins					110:117	BACKGROUND Stationary phase survival proteins	73:117	BACKGROUND Stationary phase survival proteins (Sps)	73:123	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	1	21	theme	domain	170:175	arg1	compositions					177:188	analogous domain compositions	160:188	analogous domain compositions	160:188	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	4	22	theme	lengthy	802:808	arg1	incubation					810:819	lengthy incubation	802:819	lengthy incubation during crystallisation	802:842	The protein crystallised in the presence of pentasaccharide shows a 1,6-anhydrodisaccharide sugar product, indicating that YuiC cleaves the sugar backbone to form an anhydro product at least on lengthy incubation during crystallisation.
26163297	1	23	theme	phase	95:99	arg1	Sps					120:122	Sps	120:122	Sps	120:122	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	1	23	theme	phase	95:99	arg1	proteins					110:117	BACKGROUND Stationary phase survival proteins	73:117	BACKGROUND Stationary phase survival proteins (Sps)	73:123	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	0	24	theme	stationary	17:26	arg1	YuiC					51:54	the stationary phase survival protein YuiC	13:54	the stationary phase survival protein YuiC	13:54	Structure of the stationary phase survival protein YuiC from B.subtilis.
26163297	5	25	theme	stripped	1007:1014	arg1	Actinobacteria					981:994	Actinobacteria	981:994	Actinobacteria	981:994	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	25	theme	stripped	1007:1014	arg1	versions					1021:1028	stripped down versions	1007:1028	stripped down versions of lysozyme and soluble lytic transglycosylase proteins	1007:1084	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	1	26	theme	survival	101:108	arg1	Sps					120:122	Sps	120:122	Sps	120:122	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	1	26	theme	survival	101:108	arg1	proteins					110:117	BACKGROUND Stationary phase survival proteins	73:117	BACKGROUND Stationary phase survival proteins (Sps)	73:123	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	4	27	theme	sugar	700:704	arg1	product					706:712	a 1,6-anhydrodisaccharide sugar product	674:712	a 1,6-anhydrodisaccharide sugar product	674:712	The protein crystallised in the presence of pentasaccharide shows a 1,6-anhydrodisaccharide sugar product, indicating that YuiC cleaves the sugar backbone to form an anhydro product at least on lengthy incubation during crystallisation.
26163297	1	28	theme	Actinobacteria	267:280	arg1	factors					256:262	the resuscitation promoting factors	228:262	the resuscitation promoting factors of Actinobacteria	228:280	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	2	29	theme	YuiC	399:402	arg1	structure					362:370	The first structure	352:370	The first structure of a Firmicute Sps protein YuiC	352:402	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	0	30	theme	survival	34:41	arg1	YuiC					51:54	the stationary phase survival protein YuiC	13:54	the stationary phase survival protein YuiC	13:54	Structure of the stationary phase survival protein YuiC from B.subtilis.
26163297	5	31	theme	soluble	1046:1052	arg1	transglycosylase					1060:1075	soluble lytic transglycosylase	1046:1075	soluble lytic transglycosylase	1046:1075	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	4	32	theme	anhydro	774:780	arg1	product					782:788	an anhydro product	771:788	an anhydro product	771:788	The protein crystallised in the presence of pentasaccharide shows a 1,6-anhydrodisaccharide sugar product, indicating that YuiC cleaves the sugar backbone to form an anhydro product at least on lengthy incubation during crystallisation.
26163297	2	33	theme	protein	391:397	arg1	YuiC					399:402	a Firmicute Sps protein YuiC	375:402	a Firmicute Sps protein YuiC	375:402	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	0	34	theme	phase	28:32	arg1	YuiC					51:54	the stationary phase survival protein YuiC	13:54	the stationary phase survival protein YuiC	13:54	Structure of the stationary phase survival protein YuiC from B.subtilis.
26163297	2	35	theme	MltA	502:505	arg1	version					453:459	a stripped down version	437:459	a stripped down version of the cell-wall peptidoglycan hydrolase MltA	437:505	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	0	36	from	B.subtilis	61:70	arg1	Structure					0:8	Structure	0:8	Structure of the stationary phase survival protein YuiC from B.subtilis.	0:71	Structure of the stationary phase survival protein YuiC from B.subtilis.
26163297	5	37	dep	CONCLUSIONS	845:855	arg1	simplification					872:885	The structural simplification	857:885	The structural simplification of MltA in Sps proteins	857:909	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	37	dep	CONCLUSIONS	845:855	arg1	analogous					914:922	analogous	914:922	analogous	914:922	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	2	38	theme	Sps	387:389	arg1	YuiC					399:402	a Firmicute Sps protein YuiC	375:402	a Firmicute Sps protein YuiC	375:402	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	0	39	theme	YuiC	51:54	arg1	Structure					0:8	Structure	0:8	Structure of the stationary phase survival protein YuiC from B.subtilis.	0:71	Structure of the stationary phase survival protein YuiC from B.subtilis.
26163297	5	40	theme	transglycosylase	1060:1075	arg1	proteins					1077:1084	lysozyme and soluble lytic transglycosylase proteins	1033:1084	lysozyme and soluble lytic transglycosylase proteins	1033:1084	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	41	theme	MltA	890:893	arg1	simplification					872:885	The structural simplification	857:885	The structural simplification of MltA in Sps proteins	857:909	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	41	theme	MltA	890:893	arg1	analogous					914:922	analogous	914:922	analogous	914:922	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	0	42	theme	protein	43:49	arg1	YuiC					51:54	the stationary phase survival protein YuiC	13:54	the stationary phase survival protein YuiC	13:54	Structure of the stationary phase survival protein YuiC from B.subtilis.
26163297	5	43	theme	down	1016:1019	arg1	Actinobacteria					981:994	Actinobacteria	981:994	Actinobacteria	981:994	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	43	theme	down	1016:1019	arg1	versions					1021:1028	stripped down versions	1007:1028	stripped down versions of lysozyme and soluble lytic transglycosylase proteins	1007:1084	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	3	44	theme	swapped	544:550	arg1	dimer					552:556	a domain swapped dimer	535:556	a domain swapped dimer	535:556	The YuiC structures are of a domain swapped dimer, although some monomer is also found in solution.
26163297	4	45	theme	pentasaccharide	652:666	arg1	presence					640:647	the presence	636:647	the presence of pentasaccharide	636:666	The protein crystallised in the presence of pentasaccharide shows a 1,6-anhydrodisaccharide sugar product, indicating that YuiC cleaves the sugar backbone to form an anhydro product at least on lengthy incubation during crystallisation.
26163297	1	46	theme	different	294:302	arg1	domain					336:341	a different putative peptidoglycan cleaving domain	292:341	a different putative peptidoglycan cleaving domain	292:341	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	5	47	theme	factor	963:968	arg1	Actinobacteria					981:994	Actinobacteria	981:994	Actinobacteria	981:994	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	47	theme	factor	963:968	arg1	domains					970:976	the resuscitation promoting factor domains	935:976	the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins	935:1084	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	4	48	theme	1,6-anhydrodisaccharide	676:698	arg1	product					706:712	a 1,6-anhydrodisaccharide sugar product	674:712	a 1,6-anhydrodisaccharide sugar product	674:712	The protein crystallised in the presence of pentasaccharide shows a 1,6-anhydrodisaccharide sugar product, indicating that YuiC cleaves the sugar backbone to form an anhydro product at least on lengthy incubation during crystallisation.
26163297	1	49	theme	cases	203:207	arg1	context					216:222	some cases genome context	198:222	some cases genome context	198:222	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	5	50	theme	promoting	953:961	arg1	Actinobacteria					981:994	Actinobacteria	981:994	Actinobacteria	981:994	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	50	theme	promoting	953:961	arg1	domains					970:976	the resuscitation promoting factor domains	935:976	the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins	935:1084	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	4	51	theme	sugar	748:752	arg1	backbone					754:761	the sugar backbone	744:761	the sugar backbone to form an anhydro product at least on lengthy incubation during crystallisation	744:842	The protein crystallised in the presence of pentasaccharide shows a 1,6-anhydrodisaccharide sugar product, indicating that YuiC cleaves the sugar backbone to form an anhydro product at least on lengthy incubation during crystallisation.
26163297	1	52	theme	genome	209:214	arg1	context					216:222	some cases genome context	198:222	some cases genome context	198:222	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	5	53	theme	lysozyme	1033:1040	arg1	proteins					1077:1084	lysozyme and soluble lytic transglycosylase proteins	1033:1084	lysozyme and soluble lytic transglycosylase proteins	1033:1084	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	2	54	theme	first	356:360	arg1	structure					362:370	The first structure	352:370	The first structure of a Firmicute Sps protein YuiC	352:402	RESULTS The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA.
26163297	3	55	located	found	589:593	arg1	solution					598:605	solution	598:605	solution	598:605	The YuiC structures are of a domain swapped dimer, although some monomer is also found in solution.
26163297	3	55	located	found	589:593	arg2	monomer					573:579	some monomer	568:579	some monomer	568:579	The YuiC structures are of a domain swapped dimer, although some monomer is also found in solution.
26163297	1	56	theme	putative	304:311	arg1	domain					336:341	a different putative peptidoglycan cleaving domain	292:341	a different putative peptidoglycan cleaving domain	292:341	BACKGROUND Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain.
26163297	5	57	theme	Actinobacteria	981:994	arg1	Actinobacteria					981:994	Actinobacteria	981:994	Actinobacteria	981:994	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	57	theme	Actinobacteria	981:994	arg1	domains					970:976	the resuscitation promoting factor domains	935:976	the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins	935:1084	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	58	theme	structural	861:870	arg1	simplification					872:885	The structural simplification	857:885	The structural simplification of MltA in Sps proteins	857:909	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
26163297	5	58	theme	structural	861:870	arg1	analogous					914:922	analogous	914:922	analogous	914:922	CONCLUSIONS The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
28040891	1	0	with	replacement	289:299	arg1	d-Ala-d-Lac					362:372	d-Ala-d-Lac	362:372	d-Ala-d-Lac	362:372	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	9	1	from	biosynthesis	2035:2046	arg1	VRE					2051:2053	VRE	2051:2053	VRE	2051:2053	These high percentages indicate that d-Ala-d-Lac-substituted lipid II was efficiently utilized for PG biosynthesis in VRE.
28040891	4	2	theme	biosynthesis	1084:1095	arg1	step					1073:1076	the transglycosylation step	1050:1076	the transglycosylation step of PG biosynthesis	1050:1095	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	1	3	theme	terminus	320:327	arg1	replacement					289:299	replacement	289:299	replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac	289:372	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	2	4	from	induction	481:489	arg1	Effects					446:452	Effects	446:452	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299)	446:527	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	2	4	from	induction	481:489	arg1	ATCC					517:520	ATCC 51299	517:526	ATCC 51299	517:526	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	2	4	from	induction	481:489	arg1	faecalis					507:514	Enterococcus faecalis	494:514	Enterococcus faecalis (ATCC 51299)	494:527	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	3	5	theme	accumulated	779:789	arg1	nucleotide					798:807	Park's nucleotide	791:807	accumulated Park's nucleotide	779:807	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	8	6	theme	stationary	1894:1903	arg1	OD660					1919:1923	OD660 = 1.0	1919:1929	OD660 = 1.0	1919:1929	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	8	6	theme	stationary	1894:1903	arg1	phase					1912:1916	the stationary growth phase	1890:1916	the stationary growth phase (OD660 = 1.0)	1890:1930	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	1	7	theme	peptidoglycan	332:344	arg1	stems					351:355	peptidoglycan (PG) stems	332:355	peptidoglycan (PG) stems	332:355	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	1	8	theme	resistance	227:236	arg1	Induction					203:211	Induction	203:211	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE)	203:278	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	7	9	with	Muropeptides	1588:1599	arg1	stems					1609:1613	PG stems	1606:1613	PG stems terminating in d-Ala-d-Lac	1606:1640	Muropeptides with PG stems terminating in d-Ala-d-Lac were found only in VRE grown in the presence of vancomycin.
28040891	1	10	theme	vancomycin-resistant	241:260	arg1	VRE					275:277	VRE	275:277	VRE	275:277	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	1	10	theme	vancomycin-resistant	241:260	arg1	enterococci					262:272	vancomycin-resistant enterococci	241:272	vancomycin-resistant enterococci (VRE)	241:278	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	2	11	theme	liquid	614:619	arg1	spectrometry					641:652	liquid chromatography-mass spectrometry	614:652	liquid chromatography-mass spectrometry (LC-MS)	614:660	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	2	11	theme	liquid	614:619	arg1	LC-MS					655:659	LC-MS	655:659	LC-MS	655:659	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	0	12	theme	Resonance	161:169	arg1	Analysis					193:200	a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis	121:200	a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis	121:200	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	7	13	theme	vancomycin	1690:1699	arg1	presence					1678:1685	the presence	1674:1685	the presence of vancomycin	1674:1699	Muropeptides with PG stems terminating in d-Ala-d-Lac were found only in VRE grown in the presence of vancomycin.
28040891	0	14	theme	Mass	175:178	arg1	Spectrometry					180:191	Mass Spectrometry	175:191	Mass Spectrometry	175:191	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	3	15	theme	Solid-state	672:682	arg1	NMR					684:686	Solid-state NMR	672:686	Solid-state NMR	672:686	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	9	16	used	utilized	2019:2026	arg2	lipid					1994:1998	d-Ala-d-Lac-substituted lipid II	1970:2001	d-Ala-d-Lac-substituted lipid II	1970:2001	These high percentages indicate that d-Ala-d-Lac-substituted lipid II was efficiently utilized for PG biosynthesis in VRE.
28040891	3	17	theme	=	904:904	arg1	OD660					898:902	OD660 = 0.4	898:908	OD660 = 0.4	898:908	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	17	theme	=	904:904	arg1	phase					891:895	the early exponential growth phase	862:895	the early exponential growth phase (OD660 = 0.4)	862:909	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	2	18	theme	spectrometry	641:652	arg1	analysis					662:669	a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis	554:669	a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis	554:669	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	2	19	theme	solid-state	565:575	arg1	NMR					605:607	NMR	605:607	NMR	605:607	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	2	19	theme	solid-state	565:575	arg1	resonance					594:602	solid-state nuclear magnetic resonance	565:602	solid-state nuclear magnetic resonance (NMR)	565:608	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	4	20	theme	PG	1185:1186	arg1	density					1203:1209	the PG bridge-linking density	1181:1209	the PG bridge-linking density	1181:1209	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	3	21	theme	bridge-linking	832:845	arg1	density					847:853	the PG bridge-linking density	825:853	the PG bridge-linking density	825:853	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	0	22	theme	Enterococcus	93:104	arg1	faecalis					106:113	Vancomycin-Resistant Enterococcus faecalis	72:113	Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis	72:200	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	3	23	theme	intact	914:919	arg1	cells					927:931	intact whole cells	914:931	intact whole cells of VRE	914:938	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	9	24	theme	PG	2032:2033	arg1	biosynthesis					2035:2046	PG biosynthesis	2032:2046	PG biosynthesis in VRE	2032:2053	These high percentages indicate that d-Ala-d-Lac-substituted lipid II was efficiently utilized for PG biosynthesis in VRE.
28040891	3	25	theme	growth	884:889	arg1	OD660					898:902	OD660 = 0.4	898:908	OD660 = 0.4	898:908	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	25	theme	growth	884:889	arg1	phase					891:895	the early exponential growth phase	862:895	the early exponential growth phase (OD660 = 0.4)	862:909	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	1	26	theme	binding	401:407	arg1	affinity					409:416	the binding affinity	397:416	the binding affinity of vancomycin for lipid II	397:443	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	1	27	from	Induction	203:211	arg1	VRE					275:277	VRE	275:277	VRE	275:277	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	1	27	from	Induction	203:211	arg1	enterococci					262:272	vancomycin-resistant enterococci	241:272	vancomycin-resistant enterococci (VRE)	241:278	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	0	28	from	Quantification	0:13	arg1	faecalis					106:113	Vancomycin-Resistant Enterococcus faecalis	72:113	Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis	72:200	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	5	29	theme	PG	1317:1318	arg1	bridge					1320:1325	the PG bridge	1313:1325	the PG bridge to lipid II	1313:1337	This indicated that the attachment of the PG bridge to lipid II by the peptidyl transferases was not inhibited by the d-Ala-d-Lac-substituted PG stem structure in VRE.
28040891	3	30	theme	early	866:870	arg1	OD660					898:902	OD660 = 0.4	898:908	OD660 = 0.4	898:908	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	30	theme	early	866:870	arg1	phase					891:895	the early exponential growth phase	862:895	the early exponential growth phase (OD660 = 0.4)	862:909	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	0	31	theme	Combined	123:130	arg1	Analysis					193:200	a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis	121:200	a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis	121:200	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	3	32	theme	VRE	936:938	arg1	cells					927:931	intact whole cells	914:931	intact whole cells of VRE	914:938	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	2	33	theme	magnetic	585:592	arg1	NMR					605:607	NMR	605:607	NMR	605:607	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	2	33	theme	magnetic	585:592	arg1	resonance					594:602	solid-state nuclear magnetic resonance	565:602	solid-state nuclear magnetic resonance (NMR)	565:608	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	0	34	theme	Nuclear	144:150	arg1	Resonance					161:169	Solid-State Nuclear Magnetic Resonance	132:169	Solid-State Nuclear Magnetic Resonance	132:169	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	0	35	theme	Structure	59:67	arg1	Quantification					0:13	Quantification	0:13	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.	0:201	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	4	36	theme	step	1073:1076	arg1	inhibition					1036:1045	the inhibition	1032:1045	the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance	1032:1145	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	4	37	theme	resistance	1249:1258	arg1	induction					1225:1233	the induction	1221:1233	the induction of vancomycin resistance	1221:1258	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	4	38	theme	PG	1081:1082	arg1	biosynthesis					1084:1095	PG biosynthesis	1081:1095	PG biosynthesis	1081:1095	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	6	39	theme	cell	1489:1492	arg1	walls					1494:1498	mutanolysin-digested isolated cell walls	1459:1498	mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction	1459:1560	Compositions of mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction were determined by LC-MS.
28040891	7	40	theme	PG	1606:1607	arg1	stems					1609:1613	PG stems	1606:1613	PG stems terminating in d-Ala-d-Lac	1606:1640	Muropeptides with PG stems terminating in d-Ala-d-Lac were found only in VRE grown in the presence of vancomycin.
28040891	8	41	with	muropeptides	1717:1728	arg1	stem					1750:1753	a pentapeptide stem	1735:1753	a pentapeptide stem terminating in d-Ala-d-Lac for VRE	1735:1788	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	3	42	theme	d-Lac	727:731	arg1	amounts					716:722	the total amounts	706:722	the total amounts of d-Lac and l	706:737	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	42	theme	d-Lac	727:731	arg1	changes					814:820	changes	814:820	changes to the PG bridge-linking density	814:853	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	42	theme	d-Lac	727:731	arg1	d-Lac					727:731	d-Lac	727:731	d-Lac	727:731	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	42	theme	d-Lac	727:731	arg1	nucleotide					798:807	Park's nucleotide	791:807	accumulated Park's nucleotide	779:807	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	42	theme	d-Lac	727:731	arg1	l					737:737	l	737:737	l	737:737	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	42	theme	d-Lac	727:731	arg1	d-Ala					739:743	d-Ala	739:743	d-Ala metabolized from [2-13C]pyruvate	739:776	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	2	43	theme	resistance	470:479	arg1	induction					481:489	vancomycin resistance induction	459:489	vancomycin resistance induction in Enterococcus faecalis (ATCC 51299)	459:527	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	6	44	theme	mutanolysin-digested	1459:1478	arg1	walls					1494:1498	mutanolysin-digested isolated cell walls	1459:1498	mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction	1459:1560	Compositions of mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction were determined by LC-MS.
28040891	0	45	theme	Peptidoglycan	45:57	arg1	Structure					59:67	the d-Ala-d-Lac-Terminated Peptidoglycan Structure	18:67	the d-Ala-d-Lac-Terminated Peptidoglycan Structure	18:67	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	4	46	theme	resistance	1136:1145	arg1	phase					1116:1120	the initial phase	1104:1120	the initial phase of vancomycin resistance	1104:1145	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	6	47	theme	walls	1494:1498	arg1	Compositions					1443:1454	Compositions	1443:1454	Compositions of mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction	1443:1560	Compositions of mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction were determined by LC-MS.
28040891	5	48	theme	d-Ala-d-Lac-substituted	1393:1415	arg1	structure					1425:1433	the d-Ala-d-Lac-substituted PG stem structure	1389:1433	the d-Ala-d-Lac-substituted PG stem structure in VRE	1389:1440	This indicated that the attachment of the PG bridge to lipid II by the peptidyl transferases was not inhibited by the d-Ala-d-Lac-substituted PG stem structure in VRE.
28040891	6	49	theme	vancomycin	1530:1539	arg1	resistance					1541:1550	vancomycin resistance	1530:1550	vancomycin resistance induction	1530:1560	Compositions of mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction were determined by LC-MS.
28040891	7	50	located	found	1647:1651	arg2	Muropeptides					1588:1599	Muropeptides	1588:1599	Muropeptides with PG stems terminating in d-Ala-d-Lac	1588:1640	Muropeptides with PG stems terminating in d-Ala-d-Lac were found only in VRE grown in the presence of vancomycin.
28040891	7	50	located	found	1647:1651	arg1	VRE					1661:1663	VRE	1661:1663	VRE grown in the presence of vancomycin	1661:1699	Muropeptides with PG stems terminating in d-Ala-d-Lac were found only in VRE grown in the presence of vancomycin.
28040891	5	51	theme	stem	1420:1423	arg1	structure					1425:1433	the d-Ala-d-Lac-substituted PG stem structure	1389:1433	the d-Ala-d-Lac-substituted PG stem structure in VRE	1389:1440	This indicated that the attachment of the PG bridge to lipid II by the peptidyl transferases was not inhibited by the d-Ala-d-Lac-substituted PG stem structure in VRE.
28040891	8	52	theme	pentapeptide	1737:1748	arg1	stem					1750:1753	a pentapeptide stem	1735:1753	a pentapeptide stem terminating in d-Ala-d-Lac for VRE	1735:1788	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	1	53	theme	d-Ala-d-Ala	308:318	arg1	terminus					320:327	the d-Ala-d-Ala terminus	304:327	the d-Ala-d-Ala terminus of peptidoglycan (PG) stems	304:355	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	4	54	theme	initial	1108:1114	arg1	phase					1116:1120	the initial phase	1104:1120	the initial phase of vancomycin resistance	1104:1145	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	8	55	theme	growth	1905:1910	arg1	OD660					1919:1923	OD660 = 1.0	1919:1929	OD660 = 1.0	1919:1929	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	8	55	theme	growth	1905:1910	arg1	phase					1912:1916	the stationary growth phase	1890:1916	the stationary growth phase (OD660 = 1.0)	1890:1930	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	1	56	theme	vancomycin	216:225	arg1	resistance					227:236	vancomycin resistance	216:236	vancomycin resistance	216:236	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	8	57	theme	=	1925:1925	arg1	OD660					1919:1923	OD660 = 1.0	1919:1929	OD660 = 1.0	1919:1929	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	8	57	theme	=	1925:1925	arg1	phase					1912:1916	the stationary growth phase	1890:1916	the stationary growth phase (OD660 = 1.0)	1890:1930	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	9	58	theme	high	1939:1942	arg1	percentages					1944:1954	These high percentages	1933:1954	These high percentages	1933:1954	These high percentages indicate that d-Ala-d-Lac-substituted lipid II was efficiently utilized for PG biosynthesis in VRE.
28040891	0	59	theme	Spectrometry	180:191	arg1	Analysis					193:200	a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis	121:200	a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis	121:200	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	9	60	theme	d-Ala-d-Lac-substituted	1970:1992	arg1	lipid					1994:1998	d-Ala-d-Lac-substituted lipid II	1970:2001	d-Ala-d-Lac-substituted lipid II	1970:2001	These high percentages indicate that d-Ala-d-Lac-substituted lipid II was efficiently utilized for PG biosynthesis in VRE.
28040891	3	61	theme	PG	829:830	arg1	density					847:853	the PG bridge-linking density	825:853	the PG bridge-linking density	825:853	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	8	62	theme	vancomycin	1815:1824	arg1	presence					1803:1810	the presence	1799:1810	the presence of vancomycin	1799:1824	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	4	63	theme	high	943:946	arg1	level					948:952	A high level	941:952	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance	941:1145	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	4	64	theme	vancomycin	1238:1247	arg1	resistance					1249:1258	vancomycin resistance	1238:1258	vancomycin resistance	1238:1258	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	5	65	theme	bridge	1320:1325	arg1	attachment					1299:1308	the attachment	1295:1308	the attachment of the PG bridge to lipid II by the peptidyl transferases	1295:1366	This indicated that the attachment of the PG bridge to lipid II by the peptidyl transferases was not inhibited by the d-Ala-d-Lac-substituted PG stem structure in VRE.
28040891	2	66	theme	chromatography-mass	621:639	arg1	spectrometry					641:652	liquid chromatography-mass spectrometry	614:652	liquid chromatography-mass spectrometry (LC-MS)	614:660	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	2	66	theme	chromatography-mass	621:639	arg1	LC-MS					655:659	LC-MS	655:659	LC-MS	655:659	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	4	67	theme	bridge-linking	1188:1201	arg1	density					1203:1209	the PG bridge-linking density	1181:1209	the PG bridge-linking density	1181:1209	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	2	68	theme	combined	556:563	arg1	analysis					662:669	a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis	554:669	a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis	554:669	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	4	69	theme	depsipeptide-substituted	973:996	arg1	nucleotide					1005:1014	depsipeptide-substituted Park's nucleotide	973:1014	depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance	973:1145	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	2	70	theme	resonance	594:602	arg1	analysis					662:669	a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis	554:669	a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis	554:669	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	8	71	theme	midexponential	1843:1856	arg1	OD660					1865:1869	OD660 = 0.6	1865:1875	OD660 = 0.6	1865:1875	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	8	71	theme	midexponential	1843:1856	arg1	phase					1858:1862	the midexponential phase	1839:1862	the midexponential phase (OD660 = 0.6)	1839:1876	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	3	72	theme	whole	921:925	arg1	cells					927:931	intact whole cells	914:931	intact whole cells of VRE	914:938	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	4	73	theme	consistent	1016:1025	arg1	nucleotide					1005:1014	depsipeptide-substituted Park's nucleotide	973:1014	depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance	973:1145	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	0	74	theme	Solid-State	132:142	arg1	Resonance					161:169	Solid-State Nuclear Magnetic Resonance	132:169	Solid-State Nuclear Magnetic Resonance	132:169	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	4	75	theme	accumulation	957:968	arg1	level					948:952	A high level	941:952	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance	941:1145	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	3	76	theme	exponential	872:882	arg1	OD660					898:902	OD660 = 0.4	898:908	OD660 = 0.4	898:908	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	76	theme	exponential	872:882	arg1	phase					891:895	the early exponential growth phase	862:895	the early exponential growth phase (OD660 = 0.4)	862:909	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	0	77	theme	Magnetic	152:159	arg1	Resonance					161:169	Solid-State Nuclear Magnetic Resonance	132:169	Solid-State Nuclear Magnetic Resonance	132:169	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	1	78	theme	vancomycin	421:430	arg1	affinity					409:416	the binding affinity	397:416	the binding affinity of vancomycin for lipid II	397:443	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	8	79	theme	=	1871:1871	arg1	OD660					1865:1869	OD660 = 0.6	1865:1875	OD660 = 0.6	1865:1875	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	8	79	theme	=	1871:1871	arg1	phase					1858:1862	the midexponential phase	1839:1862	the midexponential phase (OD660 = 0.6)	1839:1876	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	2	80	theme	nuclear	577:583	arg1	NMR					605:607	NMR	605:607	NMR	605:607	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	2	80	theme	nuclear	577:583	arg1	resonance					594:602	solid-state nuclear magnetic resonance	565:602	solid-state nuclear magnetic resonance (NMR)	565:608	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	6	81	theme	VRE	1503:1505	arg1	walls					1494:1498	mutanolysin-digested isolated cell walls	1459:1498	mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction	1459:1560	Compositions of mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction were determined by LC-MS.
28040891	4	82	theme	transglycosylation	1054:1071	arg1	step					1073:1076	the transglycosylation step	1050:1076	the transglycosylation step of PG biosynthesis	1050:1095	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	3	83	theme	l	737:737	arg1	amounts					716:722	the total amounts	706:722	the total amounts of d-Lac and l	706:737	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	83	theme	l	737:737	arg1	changes					814:820	changes	814:820	changes to the PG bridge-linking density	814:853	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	83	theme	l	737:737	arg1	d-Lac					727:731	d-Lac	727:731	d-Lac	727:731	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	83	theme	l	737:737	arg1	nucleotide					798:807	Park's nucleotide	791:807	accumulated Park's nucleotide	779:807	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	83	theme	l	737:737	arg1	l					737:737	l	737:737	l	737:737	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	83	theme	l	737:737	arg1	d-Ala					739:743	d-Ala	739:743	d-Ala metabolized from [2-13C]pyruvate	739:776	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	0	84	theme	d-Ala-d-Lac-Terminated	22:43	arg1	Structure					59:67	the d-Ala-d-Lac-Terminated Peptidoglycan Structure	18:67	the d-Ala-d-Lac-Terminated Peptidoglycan Structure	18:67	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	1	85	theme	PG	347:348	arg1	stems					351:355	peptidoglycan (PG) stems	332:355	peptidoglycan (PG) stems	332:355	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	5	86	theme	peptidyl	1346:1353	arg1	transferases					1355:1366	the peptidyl transferases	1342:1366	the peptidyl transferases	1342:1366	This indicated that the attachment of the PG bridge to lipid II by the peptidyl transferases was not inhibited by the d-Ala-d-Lac-substituted PG stem structure in VRE.
28040891	6	87	theme	isolated	1480:1487	arg1	walls					1494:1498	mutanolysin-digested isolated cell walls	1459:1498	mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction	1459:1560	Compositions of mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction were determined by LC-MS.
28040891	1	88	theme	stems	351:355	arg1	terminus					320:327	the d-Ala-d-Ala terminus	304:327	the d-Ala-d-Ala terminus of peptidoglycan (PG) stems	304:355	Induction of vancomycin resistance in vancomycin-resistant enterococci (VRE) involves replacement of the d-Ala-d-Ala terminus of peptidoglycan (PG) stems with d-Ala-d-Lac, dramatically reducing the binding affinity of vancomycin for lipid II.
28040891	5	89	from	structure	1425:1433	arg1	VRE					1438:1440	VRE	1438:1440	VRE	1438:1440	This indicated that the attachment of the PG bridge to lipid II by the peptidyl transferases was not inhibited by the d-Ala-d-Lac-substituted PG stem structure in VRE.
28040891	3	90	theme	total	710:714	arg1	l					737:737	l	737:737	l	737:737	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	90	theme	total	710:714	arg1	d-Lac					727:731	d-Lac	727:731	d-Lac	727:731	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	3	90	theme	total	710:714	arg1	amounts					716:722	the total amounts	706:722	the total amounts of d-Lac and l	706:737	Solid-state NMR directly measured the total amounts of d-Lac and l,d-Ala metabolized from [2-13C]pyruvate, accumulated Park's nucleotide, and changes to the PG bridge-linking density during the early exponential growth phase (OD660 = 0.4) in intact whole cells of VRE.
28040891	2	91	theme	vancomycin	459:468	arg1	induction					481:489	vancomycin resistance induction	459:489	vancomycin resistance induction in Enterococcus faecalis (ATCC 51299)	459:527	Effects from vancomycin resistance induction in Enterococcus faecalis (ATCC 51299) were characterized using a combined solid-state nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis.
28040891	0	92	theme	Vancomycin-Resistant	72:91	arg1	faecalis					106:113	Vancomycin-Resistant Enterococcus faecalis	72:113	Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis	72:200	Quantification of the d-Ala-d-Lac-Terminated Peptidoglycan Structure in Vancomycin-Resistant Enterococcus faecalis Using a Combined Solid-State Nuclear Magnetic Resonance and Mass Spectrometry Analysis.
28040891	4	93	with	consistent	1016:1025	arg1	inhibition					1036:1045	the inhibition	1032:1045	the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance	1032:1145	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	8	94	theme	muropeptides	1717:1728	arg1	Percentages					1702:1712	Percentages	1702:1712	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin	1702:1824	Percentages of muropeptides with a pentapeptide stem terminating in d-Ala-d-Lac for VRE grown in the presence of vancomycin were 26% for the midexponential phase (OD660 = 0.6) and 57% for the stationary growth phase (OD660 = 1.0).
28040891	6	95	theme	resistance	1541:1550	arg1	induction					1552:1560	vancomycin resistance induction	1530:1560	vancomycin resistance induction	1530:1560	Compositions of mutanolysin-digested isolated cell walls of VRE grown with and without vancomycin resistance induction were determined by LC-MS.
28040891	4	96	theme	vancomycin	1125:1134	arg1	resistance					1136:1145	vancomycin resistance	1125:1145	vancomycin resistance	1125:1145	A high level of accumulation of depsipeptide-substituted Park's nucleotide consistent with the inhibition of the transglycosylation step of PG biosynthesis during the initial phase of vancomycin resistance was observed, while no changes to the PG bridge-linking density following the induction of vancomycin resistance were detected.
28040891	5	97	theme	PG	1417:1418	arg1	structure					1425:1433	the d-Ala-d-Lac-substituted PG stem structure	1389:1433	the d-Ala-d-Lac-substituted PG stem structure in VRE	1389:1440	This indicated that the attachment of the PG bridge to lipid II by the peptidyl transferases was not inhibited by the d-Ala-d-Lac-substituted PG stem structure in VRE.
29050594	1	0	theme	fiber	301:305	arg1	structure					315:323	ultrafine fiber network structure	291:323	ultrafine fiber network structure	291:323	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	8	1	theme	excellent	1051:1059	arg1	property					1075:1082	excellent antibacterial property	1051:1082	excellent antibacterial property	1051:1082	Interestingly, the resultant functionalized BC membranes exhibiting excellent antibacterial property and good biocompatibility demonstrated great utility and potential as biomaterial materials.
29050594	3	2	theme	BC	482:483	arg1	membranes					485:493	functionalized BC membranes	467:493	functionalized BC membranes	467:493	In the present study, functionalized BC membranes were prepared.
29050594	7	3	theme	membrane	891:898	arg1	surfaces					900:907	membrane surfaces	891:907	membrane surfaces	891:907	HEK293 cell lines were applied to assess the biocompatibility of membrane surfaces by MTT assay and their morphologies were observed by Confocal Microscopy.
29050594	1	4	theme	unique	218:223	arg1	biocompatibility					358:373	biocompatibility	358:373	biocompatibility	358:373	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	4	theme	unique	218:223	arg1	strength					281:288	high mechanical strength	265:288	high mechanical strength	265:288	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	4	theme	unique	218:223	arg1	crystallinity					250:262	high crystallinity	245:262	high crystallinity	245:262	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	4	theme	unique	218:223	arg1	properties					225:234	its unique properties	214:234	its unique properties	214:234	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	4	theme	unique	218:223	arg1	capacity					345:352	good water holding capacity	326:352	good water holding capacity	326:352	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	4	theme	unique	218:223	arg1	structure					315:323	ultrafine fiber network structure	291:323	ultrafine fiber network structure	291:323	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	5	theme	network	307:313	arg1	structure					315:323	ultrafine fiber network structure	291:323	ultrafine fiber network structure	291:323	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	4	6	used	used	533:536	arg2	XPS					524:526	XPS	524:526	XPS	524:526	FTIR, SEM and XPS were used to characterize the chemical composition and surface morphology.
29050594	4	6	used	used	533:536	arg2	SEM					516:518	SEM	516:518	SEM	516:518	FTIR, SEM and XPS were used to characterize the chemical composition and surface morphology.
29050594	4	6	used	used	533:536	arg2	FTIR					510:513	FTIR	510:513	FTIR	510:513	FTIR, SEM and XPS were used to characterize the chemical composition and surface morphology.
29050594	6	7	theme	membranes	815:823	arg1	properties					801:810	the antibacterial properties	783:810	the antibacterial properties of membranes	783:823	Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans were used to evaluate the antibacterial properties of membranes.
29050594	8	8	theme	antibacterial	1061:1073	arg1	property					1075:1082	excellent antibacterial property	1051:1082	excellent antibacterial property	1051:1082	Interestingly, the resultant functionalized BC membranes exhibiting excellent antibacterial property and good biocompatibility demonstrated great utility and potential as biomaterial materials.
29050594	4	9	theme	chemical	558:565	arg1	composition					567:577	the chemical composition	554:577	the chemical composition	554:577	FTIR, SEM and XPS were used to characterize the chemical composition and surface morphology.
29050594	1	10	used	used	148:151	arg2	delivery					198:205	drug delivery	193:205	drug delivery	193:205	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	10	used	used	148:151	arg2	membrane					100:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	10	used	used	148:151	arg2	healing					180:186	wound healing	174:186	wound healing	174:186	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	10	used	used	148:151	arg2	biopolymer					124:133	a promising biopolymer	112:133	a promising biopolymer which can be used for tissue implants	112:171	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	11	theme	Bacterial	75:83	arg1	biopolymer					124:133	a promising biopolymer	112:133	a promising biopolymer which can be used for tissue implants	112:171	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	11	theme	Bacterial	75:83	arg1	membrane					100:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	12	theme	good	326:329	arg1	capacity					345:352	good water holding capacity	326:352	good water holding capacity	326:352	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	8	13	theme	good	1088:1091	arg1	biocompatibility					1093:1108	good biocompatibility	1088:1108	good biocompatibility	1088:1108	Interestingly, the resultant functionalized BC membranes exhibiting excellent antibacterial property and good biocompatibility demonstrated great utility and potential as biomaterial materials.
29050594	6	14	theme	antibacterial	787:799	arg1	properties					801:810	the antibacterial properties	783:810	the antibacterial properties of membranes	783:823	Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans were used to evaluate the antibacterial properties of membranes.
29050594	1	15	theme	cellulose	85:93	arg1	biopolymer					124:133	a promising biopolymer	112:133	a promising biopolymer which can be used for tissue implants	112:171	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	15	theme	cellulose	85:93	arg1	membrane					100:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	16	theme	tissue	157:162	arg1	implants					164:171	tissue implants	157:171	tissue implants	157:171	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	17	theme	water	331:335	arg1	capacity					345:352	good water holding capacity	326:352	good water holding capacity	326:352	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	5	18	theme	Static	603:608	arg1	angles					624:629	Static water contact angles	603:629	Static water contact angles	603:629	Static water contact angles were measured to investigate surface wettability.
29050594	8	19	theme	functionalized	1012:1025	arg1	membranes					1030:1038	the resultant functionalized BC membranes	998:1038	the resultant functionalized BC membranes exhibiting excellent antibacterial property and good biocompatibility	998:1108	Interestingly, the resultant functionalized BC membranes exhibiting excellent antibacterial property and good biocompatibility demonstrated great utility and potential as biomaterial materials.
29050594	0	20	theme	bioactive	6:14	arg1	functionalization					24:40	Novel bioactive surface functionalization	0:40	Novel bioactive surface functionalization of bacterial cellulose membrane	0:72	Novel bioactive surface functionalization of bacterial cellulose membrane.
29050594	1	21	theme	holding	337:343	arg1	capacity					345:352	good water holding capacity	326:352	good water holding capacity	326:352	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	5	22	theme	water	610:614	arg1	angles					624:629	Static water contact angles	603:629	Static water contact angles	603:629	Static water contact angles were measured to investigate surface wettability.
29050594	8	23	theme	resultant	1002:1010	arg1	membranes					1030:1038	the resultant functionalized BC membranes	998:1038	the resultant functionalized BC membranes exhibiting excellent antibacterial property and good biocompatibility	998:1108	Interestingly, the resultant functionalized BC membranes exhibiting excellent antibacterial property and good biocompatibility demonstrated great utility and potential as biomaterial materials.
29050594	1	24	theme	BC	96:97	arg1	biopolymer					124:133	a promising biopolymer	112:133	a promising biopolymer which can be used for tissue implants	112:171	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	24	theme	BC	96:97	arg1	membrane					100:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	0	25	theme	Novel	0:4	arg1	functionalization					24:40	Novel bioactive surface functionalization	0:40	Novel bioactive surface functionalization of bacterial cellulose membrane	0:72	Novel bioactive surface functionalization of bacterial cellulose membrane.
29050594	5	26	theme	contact	616:622	arg1	angles					624:629	Static water contact angles	603:629	Static water contact angles	603:629	Static water contact angles were measured to investigate surface wettability.
29050594	7	27	theme	cell	833:836	arg1	lines					838:842	HEK293 cell lines	826:842	HEK293 cell lines	826:842	HEK293 cell lines were applied to assess the biocompatibility of membrane surfaces by MTT assay and their morphologies were observed by Confocal Microscopy.
29050594	1	28	theme	wound	174:178	arg1	healing					180:186	wound healing	174:186	wound healing	174:186	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	29	theme	high	245:248	arg1	crystallinity					250:262	high crystallinity	245:262	high crystallinity	245:262	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	8	30	theme	BC	1027:1028	arg1	membranes					1030:1038	the resultant functionalized BC membranes	998:1038	the resultant functionalized BC membranes exhibiting excellent antibacterial property and good biocompatibility	998:1108	Interestingly, the resultant functionalized BC membranes exhibiting excellent antibacterial property and good biocompatibility demonstrated great utility and potential as biomaterial materials.
29050594	7	31	theme	Confocal	962:969	arg1	Microscopy					971:980	Confocal Microscopy	962:980	Confocal Microscopy	962:980	HEK293 cell lines were applied to assess the biocompatibility of membrane surfaces by MTT assay and their morphologies were observed by Confocal Microscopy.
29050594	0	32	theme	surface	16:22	arg1	functionalization					24:40	Novel bioactive surface functionalization	0:40	Novel bioactive surface functionalization of bacterial cellulose membrane	0:72	Novel bioactive surface functionalization of bacterial cellulose membrane.
29050594	8	33	theme	great	1123:1127	arg1	utility					1129:1135	utility	1129:1135	utility	1129:1135	Interestingly, the resultant functionalized BC membranes exhibiting excellent antibacterial property and good biocompatibility demonstrated great utility and potential as biomaterial materials.
29050594	0	34	theme	bacterial	45:53	arg1	membrane					65:72	bacterial cellulose membrane	45:72	bacterial cellulose membrane	45:72	Novel bioactive surface functionalization of bacterial cellulose membrane.
29050594	3	35	theme	functionalized	467:480	arg1	membranes					485:493	functionalized BC membranes	467:493	functionalized BC membranes	467:493	In the present study, functionalized BC membranes were prepared.
29050594	7	36	theme	HEK293	826:831	arg1	lines					838:842	HEK293 cell lines	826:842	HEK293 cell lines	826:842	HEK293 cell lines were applied to assess the biocompatibility of membrane surfaces by MTT assay and their morphologies were observed by Confocal Microscopy.
29050594	6	37	used	used	766:769	arg2	aureus					714:719	Staphylococcus aureus	699:719	Staphylococcus aureus	699:719	Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans were used to evaluate the antibacterial properties of membranes.
29050594	6	37	used	used	766:769	arg2	coli					693:696	Escherichia coli	681:696	Escherichia coli	681:696	Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans were used to evaluate the antibacterial properties of membranes.
29050594	6	37	used	used	766:769	arg2	albicans					752:759	Candida albicans	744:759	Candida albicans	744:759	Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans were used to evaluate the antibacterial properties of membranes.
29050594	6	37	used	used	766:769	arg2	subtilis					731:738	Bacillus subtilis	722:738	Bacillus subtilis	722:738	Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans were used to evaluate the antibacterial properties of membranes.
29050594	1	38	theme	high	265:268	arg1	strength					281:288	high mechanical strength	265:288	high mechanical strength	265:288	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	39	theme	drug	193:196	arg1	delivery					198:205	drug delivery	193:205	drug delivery	193:205	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	0	40	theme	membrane	65:72	arg1	functionalization					24:40	Novel bioactive surface functionalization	0:40	Novel bioactive surface functionalization of bacterial cellulose membrane	0:72	Novel bioactive surface functionalization of bacterial cellulose membrane.
29050594	1	41	theme	mechanical	270:279	arg1	strength					281:288	high mechanical strength	265:288	high mechanical strength	265:288	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	8	42	theme	biomaterial	1154:1164	arg1	materials					1166:1174	biomaterial materials	1154:1174	biomaterial materials	1154:1174	Interestingly, the resultant functionalized BC membranes exhibiting excellent antibacterial property and good biocompatibility demonstrated great utility and potential as biomaterial materials.
29050594	0	43	theme	cellulose	55:63	arg1	membrane					65:72	bacterial cellulose membrane	45:72	bacterial cellulose membrane	45:72	Novel bioactive surface functionalization of bacterial cellulose membrane.
29050594	5	44	theme	surface	660:666	arg1	wettability					668:678	surface wettability	660:678	surface wettability	660:678	Static water contact angles were measured to investigate surface wettability.
29050594	6	45	theme	Candida	744:750	arg1	albicans					752:759	Candida albicans	744:759	Candida albicans	744:759	Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans were used to evaluate the antibacterial properties of membranes.
29050594	1	46	theme	promising	114:122	arg1	biopolymer					124:133	a promising biopolymer	112:133	a promising biopolymer which can be used for tissue implants	112:171	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	46	theme	promising	114:122	arg1	membrane					100:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	7	47	theme	MTT	912:914	arg1	assay					916:920	MTT assay	912:920	MTT assay	912:920	HEK293 cell lines were applied to assess the biocompatibility of membrane surfaces by MTT assay and their morphologies were observed by Confocal Microscopy.
29050594	3	48	theme	present	452:458	arg1	study					460:464	the present study	448:464	the present study	448:464	In the present study, functionalized BC membranes were prepared.
29050594	7	49	theme	surfaces	900:907	arg1	biocompatibility					871:886	the biocompatibility	867:886	the biocompatibility of membrane surfaces by MTT assay	867:920	HEK293 cell lines were applied to assess the biocompatibility of membrane surfaces by MTT assay and their morphologies were observed by Confocal Microscopy.
29050594	1	50	theme	due	207:209	arg1	biopolymer					124:133	a promising biopolymer	112:133	a promising biopolymer which can be used for tissue implants	112:171	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	50	theme	due	207:209	arg1	membrane					100:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane	75:107	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	1	51	theme	ultrafine	291:299	arg1	structure					315:323	ultrafine fiber network structure	291:323	ultrafine fiber network structure	291:323	Bacterial cellulose (BC) membrane is a promising biopolymer which can be used for tissue implants, wound healing, and drug delivery due to its unique properties, such as high crystallinity, high mechanical strength, ultrafine fiber network structure, good water holding capacity and biocompatibility.
29050594	2	52	theme	antibacterial	419:431	arg1	properties					433:442	antibacterial properties	419:442	antibacterial properties	419:442	However, BC does not intrinsically present antibacterial properties.
29050594	4	53	theme	surface	583:589	arg1	morphology					591:600	surface morphology	583:600	surface morphology	583:600	FTIR, SEM and XPS were used to characterize the chemical composition and surface morphology.
28748321	2	0	theme	detergent	466:474	arg1	fraction					484:491	neutral detergent soluble fraction	458:491	neutral detergent soluble fraction	458:491	The results showed that aqueous ammonia played a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose under moderate temperatures.
28748321	1	1	theme	pore	317:320	arg1	distribution					327:338	pore size distribution	317:338	pore size distribution	317:338	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	1	2	theme	enzymatic	162:170	arg1	hydrolysis					172:181	enzymatic hydrolysis	162:181	enzymatic hydrolysis of sugar beet pulp (SBP)	162:206	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	1	3	theme	pretreatment	230:241	arg1	mechanism					217:225	the mechanism	213:225	the mechanism of pretreatment	213:241	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	2	4	theme	neutral	458:464	arg1	fraction					484:491	neutral detergent soluble fraction	458:491	neutral detergent soluble fraction	458:491	The results showed that aqueous ammonia played a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose under moderate temperatures.
28748321	1	5	theme	size	322:325	arg1	distribution					327:338	pore size distribution	317:338	pore size distribution	317:338	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	3	6	theme	specific	635:642	arg1	area					652:655	specific surface area	635:655	specific surface area	635:655	Apertures of various sizes in the SBP tissues were formed, specific surface area was increased, and the fiber became rougher after pretreatment.
28748321	5	7	theme	ethanol	1026:1032	arg1	production					1034:1043	ethanol production	1026:1043	ethanol production from SBP	1026:1052	The aqueous ammonia pretreatment improved the enzymatic digestibility of SBP, which was a promising method that might be explored in ethanol production from SBP.
28748321	1	8	theme	distribution	327:338	arg1	terms					260:264	terms	260:264	terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure	260:379	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	4	9	theme	sugar	742:746	arg1	yield					748:752	The highest reducing sugar yield	721:752	The highest reducing sugar yield	721:752	The highest reducing sugar yield reached 448.52 mg/g when the SBP was pretreated by aqueous ammonia at 80 °C for 6 h, which was 2.42 times higher than that of the raw SBP.
28748321	5	10	theme	ammonia	905:911	arg1	pretreatment					913:924	The aqueous ammonia pretreatment	893:924	The aqueous ammonia pretreatment	893:924	The aqueous ammonia pretreatment improved the enzymatic digestibility of SBP, which was a promising method that might be explored in ethanol production from SBP.
28748321	4	11	theme	reducing	733:740	arg1	yield					748:752	The highest reducing sugar yield	721:752	The highest reducing sugar yield	721:752	The highest reducing sugar yield reached 448.52 mg/g when the SBP was pretreated by aqueous ammonia at 80 °C for 6 h, which was 2.42 times higher than that of the raw SBP.
28748321	1	12	theme	crystallinity	341:353	arg1	index					355:359	crystallinity index	341:359	crystallinity index	341:359	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	1	13	theme	index	355:359	arg1	terms					260:264	terms	260:264	terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure	260:379	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	1	14	theme	sugar	186:190	arg1	SBP					203:205	SBP	203:205	SBP	203:205	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	1	14	theme	sugar	186:190	arg1	pulp					197:200	sugar beet pulp	186:200	sugar beet pulp (SBP)	186:206	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	2	15	theme	vital	431:435	arg1	role					437:440	a vital role	429:440	a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose	429:545	The results showed that aqueous ammonia played a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose under moderate temperatures.
28748321	2	16	theme	fraction	484:491	arg1	degrading					445:453	degrading	445:453	degrading of neutral detergent soluble fraction	445:491	The results showed that aqueous ammonia played a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose under moderate temperatures.
28748321	1	17	theme	Aqueous	83:89	arg1	pretreatment					99:110	Aqueous ammonia pretreatment	83:110	Aqueous ammonia pretreatment under various temperatures	83:137	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	2	18	from	role	437:440	arg1	degrading					445:453	degrading	445:453	degrading of neutral detergent soluble fraction	445:491	The results showed that aqueous ammonia played a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose under moderate temperatures.
28748321	1	19	theme	beet	192:195	arg1	SBP					203:205	SBP	203:205	SBP	203:205	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	1	19	theme	beet	192:195	arg1	pulp					197:200	sugar beet pulp	186:200	sugar beet pulp (SBP)	186:206	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	0	20	theme	Aqueous	0:6	arg1	ammonia					8:14	Aqueous ammonia	0:14	Aqueous ammonia	0:14	Aqueous ammonia pretreatment of sugar beet pulp for enhanced enzymatic hydrolysis.
28748321	1	21	theme	ammonia	91:97	arg1	pretreatment					99:110	Aqueous ammonia pretreatment	83:110	Aqueous ammonia pretreatment under various temperatures	83:137	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	1	22	theme	pulp	197:200	arg1	hydrolysis					172:181	enzymatic hydrolysis	162:181	enzymatic hydrolysis of sugar beet pulp (SBP)	162:206	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	4	23	theme	highest	725:731	arg1	yield					748:752	The highest reducing sugar yield	721:752	The highest reducing sugar yield	721:752	The highest reducing sugar yield reached 448.52 mg/g when the SBP was pretreated by aqueous ammonia at 80 °C for 6 h, which was 2.42 times higher than that of the raw SBP.
28748321	3	24	from	Apertures	576:584	arg1	tissues					614:620	the SBP tissues	606:620	the SBP tissues	606:620	Apertures of various sizes in the SBP tissues were formed, specific surface area was increased, and the fiber became rougher after pretreatment.
28748321	2	25	theme	aqueous	406:412	arg1	ammonia					414:420	aqueous ammonia	406:420	aqueous ammonia	406:420	The results showed that aqueous ammonia played a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose under moderate temperatures.
28748321	5	26	theme	aqueous	897:903	arg1	pretreatment					913:924	The aqueous ammonia pretreatment	893:924	The aqueous ammonia pretreatment	893:924	The aqueous ammonia pretreatment improved the enzymatic digestibility of SBP, which was a promising method that might be explored in ethanol production from SBP.
28748321	0	27	theme	beet	38:41	arg1	pulp					43:46	sugar beet pulp	32:46	sugar beet pulp	32:46	Aqueous ammonia pretreatment of sugar beet pulp for enhanced enzymatic hydrolysis.
28748321	1	28	theme	enzymatic	273:281	arg1	hydrolysis					283:292	the enzymatic hydrolysis	269:292	the enzymatic hydrolysis	269:292	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	4	29	theme	raw	884:886	arg1	SBP					888:890	the raw SBP	880:890	the raw SBP	880:890	The highest reducing sugar yield reached 448.52 mg/g when the SBP was pretreated by aqueous ammonia at 80 °C for 6 h, which was 2.42 times higher than that of the raw SBP.
28748321	1	30	theme	microstructure	366:379	arg1	terms					260:264	terms	260:264	terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure	260:379	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	0	31	theme	sugar	32:36	arg1	pulp					43:46	sugar beet pulp	32:46	sugar beet pulp	32:46	Aqueous ammonia pretreatment of sugar beet pulp for enhanced enzymatic hydrolysis.
28748321	1	32	theme	hydrolysis	283:292	arg1	terms					260:264	terms	260:264	terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure	260:379	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	3	33	theme	SBP	610:612	arg1	tissues					614:620	the SBP tissues	606:620	the SBP tissues	606:620	Apertures of various sizes in the SBP tissues were formed, specific surface area was increased, and the fiber became rougher after pretreatment.
28748321	3	34	theme	various	589:595	arg1	sizes					597:601	various sizes	589:601	various sizes	589:601	Apertures of various sizes in the SBP tissues were formed, specific surface area was increased, and the fiber became rougher after pretreatment.
28748321	2	35	theme	soluble	476:482	arg1	fraction					484:491	neutral detergent soluble fraction	458:491	neutral detergent soluble fraction	458:491	The results showed that aqueous ammonia played a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose under moderate temperatures.
28748321	4	36	theme	aqueous	805:811	arg1	ammonia					813:819	aqueous ammonia	805:819	aqueous ammonia	805:819	The highest reducing sugar yield reached 448.52 mg/g when the SBP was pretreated by aqueous ammonia at 80 °C for 6 h, which was 2.42 times higher than that of the raw SBP.
28748321	1	37	theme	various	118:124	arg1	temperatures					126:137	various temperatures	118:137	various temperatures	118:137	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	0	38	theme	pulp	43:46	arg1	pretreatment					16:27	pretreatment	16:27	pretreatment of sugar beet pulp for enhanced enzymatic hydrolysis	16:80	Aqueous ammonia pretreatment of sugar beet pulp for enhanced enzymatic hydrolysis.
28748321	1	39	theme	chemical	295:302	arg1	composition					304:314	chemical composition	295:314	chemical composition	295:314	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	2	40	theme	moderate	553:560	arg1	temperatures					562:573	moderate temperatures	553:573	moderate temperatures	553:573	The results showed that aqueous ammonia played a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose under moderate temperatures.
28748321	5	41	theme	promising	983:991	arg1	method					993:998	a promising method	981:998	a promising method that might be explored in ethanol production from SBP	981:1052	The aqueous ammonia pretreatment improved the enzymatic digestibility of SBP, which was a promising method that might be explored in ethanol production from SBP.
28748321	5	41	theme	promising	983:991	arg1	digestibility					949:961	the enzymatic digestibility	935:961	the enzymatic digestibility	935:961	The aqueous ammonia pretreatment improved the enzymatic digestibility of SBP, which was a promising method that might be explored in ethanol production from SBP.
28748321	1	42	theme	composition	304:314	arg1	terms					260:264	terms	260:264	terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure	260:379	Aqueous ammonia pretreatment under various temperatures was applied to enhance enzymatic hydrolysis of sugar beet pulp (SBP), and the mechanism of pretreatment was evaluated in terms of the enzymatic hydrolysis, chemical composition, pore size distribution, crystallinity index, and microstructure.
28748321	5	43	theme	enzymatic	939:947	arg1	digestibility					949:961	the enzymatic digestibility	935:961	the enzymatic digestibility	935:961	The aqueous ammonia pretreatment improved the enzymatic digestibility of SBP, which was a promising method that might be explored in ethanol production from SBP.
28748321	5	43	theme	enzymatic	939:947	arg1	method					993:998	a promising method	981:998	a promising method that might be explored in ethanol production from SBP	981:1052	The aqueous ammonia pretreatment improved the enzymatic digestibility of SBP, which was a promising method that might be explored in ethanol production from SBP.
28748321	2	44	theme	cellulose	537:545	arg1	role					437:440	a vital role	429:440	a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose	429:545	The results showed that aqueous ammonia played a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose under moderate temperatures.
28748321	2	44	theme	cellulose	537:545	arg1	softening					504:512	softening	504:512	softening	504:512	The results showed that aqueous ammonia played a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose under moderate temperatures.
28748321	2	45	from	softening	504:512	arg1	degrading					445:453	degrading	445:453	degrading of neutral detergent soluble fraction	445:491	The results showed that aqueous ammonia played a vital role in degrading of neutral detergent soluble fraction as well as softening and partly exposing of cellulose under moderate temperatures.
28748321	0	46	dep	pretreatment	16:27	arg1	ammonia					8:14	Aqueous ammonia	0:14	Aqueous ammonia	0:14	Aqueous ammonia pretreatment of sugar beet pulp for enhanced enzymatic hydrolysis.
28748321	0	47	theme	enzymatic	61:69	arg1	hydrolysis					71:80	enhanced enzymatic hydrolysis	52:80	enhanced enzymatic hydrolysis	52:80	Aqueous ammonia pretreatment of sugar beet pulp for enhanced enzymatic hydrolysis.
28748321	3	48	theme	surface	644:650	arg1	area					652:655	specific surface area	635:655	specific surface area	635:655	Apertures of various sizes in the SBP tissues were formed, specific surface area was increased, and the fiber became rougher after pretreatment.
28748321	3	49	theme	sizes	597:601	arg1	Apertures					576:584	Apertures	576:584	Apertures of various sizes in the SBP tissues	576:620	Apertures of various sizes in the SBP tissues were formed, specific surface area was increased, and the fiber became rougher after pretreatment.
28748321	0	50	theme	enhanced	52:59	arg1	hydrolysis					71:80	enhanced enzymatic hydrolysis	52:80	enhanced enzymatic hydrolysis	52:80	Aqueous ammonia pretreatment of sugar beet pulp for enhanced enzymatic hydrolysis.
28748321	5	51	theme	SBP	966:968	arg1	digestibility					949:961	the enzymatic digestibility	935:961	the enzymatic digestibility	935:961	The aqueous ammonia pretreatment improved the enzymatic digestibility of SBP, which was a promising method that might be explored in ethanol production from SBP.
28748321	5	51	theme	SBP	966:968	arg1	method					993:998	a promising method	981:998	a promising method that might be explored in ethanol production from SBP	981:1052	The aqueous ammonia pretreatment improved the enzymatic digestibility of SBP, which was a promising method that might be explored in ethanol production from SBP.
28748321	5	52	from	SBP	1050:1052	arg1	production					1034:1043	ethanol production	1026:1043	ethanol production from SBP	1026:1052	The aqueous ammonia pretreatment improved the enzymatic digestibility of SBP, which was a promising method that might be explored in ethanol production from SBP.
29032456	8	0	theme	MG-63	1729:1733	arg1	cells					1735:1739	MG-63 cells	1729:1739	MG-63 cells	1729:1739	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	1	1	theme	surface	374:380	arg1	properties					348:357	antimicrobial properties	334:357	antimicrobial properties of the implant surface prior to its insertion	334:403	The present work focuses on the idea to prevent and/or inhibit the colonization of implant surfaces by microbial pathogens responsible for post-operative infections, adjusting antimicrobial properties of the implant surface prior to its insertion.
29032456	6	2	from	environment	1106:1116	arg1	terms					1121:1125	terms	1121:1125	terms of compatibility and antibacterial properties	1121:1171	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	6	3	theme	biological	1095:1104	arg1	environment					1106:1116	the biological environment	1091:1116	the biological environment in terms of compatibility and antibacterial properties	1091:1171	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	1	4	theme	microbial	261:269	arg1	pathogens					271:279	microbial pathogens	261:279	microbial pathogens responsible for post-operative infections	261:321	The present work focuses on the idea to prevent and/or inhibit the colonization of implant surfaces by microbial pathogens responsible for post-operative infections, adjusting antimicrobial properties of the implant surface prior to its insertion.
29032456	0	5	theme	implant-associated	74:91	arg1	infections					93:102	face titanium implant-associated infections	60:102	face titanium implant-associated infections	60:102	Silver-loaded chitosan coating as an integrated approach to face titanium implant-associated infections: analytical characterization and biological activity.
29032456	1	6	theme	prior	382:386	arg1	surface					374:380	the implant surface	362:380	the implant surface prior to its insertion	362:403	The present work focuses on the idea to prevent and/or inhibit the colonization of implant surfaces by microbial pathogens responsible for post-operative infections, adjusting antimicrobial properties of the implant surface prior to its insertion.
29032456	6	7	theme	compatibility	1130:1142	arg1	terms					1121:1125	terms	1121:1125	terms of compatibility and antibacterial properties	1121:1171	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	8	8	theme	bacterial	1699:1707	arg1	growth					1709:1714	bacterial growth	1699:1714	bacterial growth	1699:1714	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	0	9	theme	analytical	105:114	arg1	characterization					116:131	analytical characterization	105:131	analytical characterization	105:131	Silver-loaded chitosan coating as an integrated approach to face titanium implant-associated infections: analytical characterization and biological activity.
29032456	8	10	theme	prepared	1532:1539	arg1	coating					1541:1547	the prepared coating	1528:1547	the prepared coating	1528:1547	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	4	11	from	microscopy	801:810	arg1	mode					836:839	intermittent contact mode	815:839	intermittent contact mode (ICAFM)	815:847	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	4	11	from	microscopy	801:810	arg1	ICAFM					842:846	ICAFM	842:846	ICAFM	842:846	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	4	12	theme	force	795:799	arg1	microscopy					801:810	atomic force microscopy	788:810	atomic force microscopy in intermittent contact mode (ICAFM)	788:847	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	1	13	theme	responsible	281:291	arg1	pathogens					271:279	microbial pathogens	261:279	microbial pathogens responsible for post-operative infections	261:321	The present work focuses on the idea to prevent and/or inhibit the colonization of implant surfaces by microbial pathogens responsible for post-operative infections, adjusting antimicrobial properties of the implant surface prior to its insertion.
29032456	8	14	dep	released	1639:1646	arg1	concentration					1615:1627	the low concentration	1607:1627	the low concentration of silver released (below 0.1 ppm up to 21 days)	1607:1676	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	8	14	dep	released	1639:1646	arg1	0.1 ppm					1655:1661	0.1 ppm	1655:1661	0.1 ppm	1655:1661	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	4	15	theme	atomic	788:793	arg1	microscopy					801:810	atomic force microscopy	788:810	atomic force microscopy in intermittent contact mode (ICAFM)	788:847	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	0	16	dep	characterization	116:131	arg1	chitosan					14:21	Silver-loaded chitosan	0:21	Silver-loaded chitosan coating as an integrated approach to face titanium implant-associated infections	0:102	Silver-loaded chitosan coating as an integrated approach to face titanium implant-associated infections: analytical characterization and biological activity.
29032456	7	17	used	used	1393:1396	arg2	coating					1309:1315	a coating	1307:1315	a coating showing a silver surface atomic percentage equal to 0.3%	1307:1372	These studies revealed that a coating showing a silver surface atomic percentage equal to 0.3% can be effectively used as antibacterial system, while providing good viability of osteoblast-like cells after 7 days.
29032456	7	17	used	used	1393:1396	arg2	system					1415:1420	antibacterial system	1401:1420	antibacterial system	1401:1420	These studies revealed that a coating showing a silver surface atomic percentage equal to 0.3% can be effectively used as antibacterial system, while providing good viability of osteoblast-like cells after 7 days.
29032456	7	18	theme	silver	1327:1332	arg1	percentage					1349:1358	a silver surface atomic percentage	1325:1358	a silver surface atomic percentage equal to 0.3%	1325:1372	These studies revealed that a coating showing a silver surface atomic percentage equal to 0.3% can be effectively used as antibacterial system, while providing good viability of osteoblast-like cells after 7 days.
29032456	3	19	theme	silver	563:568	arg1	salt					570:573	a silver salt	561:573	a silver salt	561:573	When a silver salt was added during the chitosan deposition step, a stable and scalable silver incorporation was achieved.
29032456	4	20	used	used	853:856	arg2	microscopy					801:810	atomic force microscopy	788:810	atomic force microscopy in intermittent contact mode (ICAFM)	788:847	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	7	21	theme	osteoblast-like	1457:1471	arg1	cells					1473:1477	osteoblast-like cells	1457:1477	osteoblast-like cells	1457:1477	These studies revealed that a coating showing a silver surface atomic percentage equal to 0.3% can be effectively used as antibacterial system, while providing good viability of osteoblast-like cells after 7 days.
29032456	2	22	theme	acrylic	514:520	arg1	poly					509:512	poly	509:512	poly(acrylic acid)-coated titanium substrates	509:553	An antibacterial coating based on chitosan and silver was developed by electrodeposition techniques on poly(acrylic acid)-coated titanium substrates.
29032456	2	22	theme	acrylic	514:520	arg1	acid					522:525	acrylic acid	514:525	acrylic acid	514:525	An antibacterial coating based on chitosan and silver was developed by electrodeposition techniques on poly(acrylic acid)-coated titanium substrates.
29032456	0	23	theme	Silver-loaded	0:12	arg1	chitosan					14:21	Silver-loaded chitosan	0:21	Silver-loaded chitosan coating as an integrated approach to face titanium implant-associated infections	0:102	Silver-loaded chitosan coating as an integrated approach to face titanium implant-associated infections: analytical characterization and biological activity.
29032456	7	24	theme	cells	1473:1477	arg1	viability					1444:1452	good viability	1439:1452	good viability of osteoblast-like cells	1439:1477	These studies revealed that a coating showing a silver surface atomic percentage equal to 0.3% can be effectively used as antibacterial system, while providing good viability of osteoblast-like cells after 7 days.
29032456	4	25	theme	physico-chemical	683:698	arg1	composition					700:710	The physico-chemical composition	679:710	The physico-chemical composition of the coating	679:725	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	5	26	attach	released	914:921	arg2	amount					897:902	The amount	893:902	The amount of silver released from the coating up to 21 days	893:952	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	5	26	attach	released	914:921	arg1	up					940:941	the coating up	928:941	the coating up to 21 days	928:952	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	5	26	attach	released	914:921	arg2	silver					907:912	silver	907:912	silver	907:912	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	4	27	theme	coating	873:879	arg1	morphology					881:890	the coating morphology	869:890	the coating morphology	869:890	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	8	28	theme	contact	1571:1577	arg1	mechanism					1587:1595	a contact killing mechanism	1569:1595	a contact killing mechanism	1569:1595	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	3	29	theme	chitosan	596:603	arg1	step					616:619	the chitosan deposition step	592:619	the chitosan deposition step	592:619	When a silver salt was added during the chitosan deposition step, a stable and scalable silver incorporation was achieved.
29032456	7	30	theme	surface	1334:1340	arg1	percentage					1349:1358	a silver surface atomic percentage	1325:1358	a silver surface atomic percentage equal to 0.3%	1325:1372	These studies revealed that a coating showing a silver surface atomic percentage equal to 0.3% can be effectively used as antibacterial system, while providing good viability of osteoblast-like cells after 7 days.
29032456	2	31	theme	electrodeposition	477:493	arg1	techniques					495:504	electrodeposition techniques	477:504	electrodeposition techniques on poly(acrylic acid)-coated titanium substrates	477:553	An antibacterial coating based on chitosan and silver was developed by electrodeposition techniques on poly(acrylic acid)-coated titanium substrates.
29032456	1	32	theme	post-operative	297:310	arg1	infections					312:321	post-operative infections	297:321	post-operative infections	297:321	The present work focuses on the idea to prevent and/or inhibit the colonization of implant surfaces by microbial pathogens responsible for post-operative infections, adjusting antimicrobial properties of the implant surface prior to its insertion.
29032456	8	33	theme	antibacterial	1497:1509	arg1	effectiveness					1511:1523	The antibacterial effectiveness	1493:1523	The antibacterial effectiveness of the prepared coating	1493:1547	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	3	34	theme	deposition	605:614	arg1	step					616:619	the chitosan deposition step	592:619	the chitosan deposition step	592:619	When a silver salt was added during the chitosan deposition step, a stable and scalable silver incorporation was achieved.
29032456	2	35	theme	antibacterial	409:421	arg1	coating					423:429	An antibacterial coating	406:429	An antibacterial coating based on chitosan and silver	406:458	An antibacterial coating based on chitosan and silver was developed by electrodeposition techniques on poly(acrylic acid)-coated titanium substrates.
29032456	6	36	theme	properties	1162:1171	arg1	terms					1121:1125	terms	1121:1125	terms of compatibility and antibacterial properties	1121:1171	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	0	37	theme	biological	137:146	arg1	activity					148:155	biological activity	137:155	biological activity	137:155	Silver-loaded chitosan coating as an integrated approach to face titanium implant-associated infections: analytical characterization and biological activity.
29032456	6	38	theme	cell	1214:1217	arg1	line					1219:1222	MG-63 osteoblast-like cell line	1192:1222	MG-63 osteoblast-like cell line	1192:1222	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	6	39	theme	antibacterial	1148:1160	arg1	properties					1162:1171	antibacterial properties	1148:1171	antibacterial properties	1148:1171	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	6	40	theme	coating	1057:1063	arg1	capability					1030:1039	The capability	1026:1039	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties	1026:1171	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	0	41	theme	integrated	37:46	arg1	approach					48:55	an integrated approach	34:55	an integrated approach to face titanium implant-associated infections	34:102	Silver-loaded chitosan coating as an integrated approach to face titanium implant-associated infections: analytical characterization and biological activity.
29032456	5	42	theme	coating	932:938	arg1	up					940:941	the coating up	928:941	the coating up to 21 days	928:952	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	8	43	theme	low	1611:1613	arg1	concentration					1615:1627	the low concentration	1607:1627	the low concentration of silver released (below 0.1 ppm up to 21 days)	1607:1676	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	8	43	theme	low	1611:1613	arg1	0.1 ppm					1655:1661	0.1 ppm	1655:1661	0.1 ppm	1655:1661	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	7	44	theme	atomic	1342:1347	arg1	percentage					1349:1358	a silver surface atomic percentage	1325:1358	a silver surface atomic percentage equal to 0.3%	1325:1372	These studies revealed that a coating showing a silver surface atomic percentage equal to 0.3% can be effectively used as antibacterial system, while providing good viability of osteoblast-like cells after 7 days.
29032456	6	45	theme	proposed	1048:1055	arg1	coating					1057:1063	the proposed coating	1044:1063	the proposed coating	1044:1063	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	1	46	theme	antimicrobial	334:346	arg1	properties					348:357	antimicrobial properties	334:357	antimicrobial properties of the implant surface prior to its insertion	334:403	The present work focuses on the idea to prevent and/or inhibit the colonization of implant surfaces by microbial pathogens responsible for post-operative infections, adjusting antimicrobial properties of the implant surface prior to its insertion.
29032456	3	47	theme	stable	624:629	arg1	incorporation					651:663	a stable and scalable silver incorporation	622:663	a stable and scalable silver incorporation	622:663	When a silver salt was added during the chitosan deposition step, a stable and scalable silver incorporation was achieved.
29032456	2	48	theme	poly	509:512	arg1	substrates					544:553	poly(acrylic acid)-coated titanium substrates	509:553	poly(acrylic acid)-coated titanium substrates	509:553	An antibacterial coating based on chitosan and silver was developed by electrodeposition techniques on poly(acrylic acid)-coated titanium substrates.
29032456	5	49	theme	coupled	983:989	arg1	ICP-MS					1017:1022	ICP-MS	1017:1022	ICP-MS	1017:1022	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	5	49	theme	coupled	983:989	arg1	spectrometry					1003:1014	inductively coupled plasma mass spectrometry	971:1014	inductively coupled plasma mass spectrometry (ICP-MS)	971:1023	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	4	50	theme	photoelectron	748:760	arg1	XPS					776:778	XPS	776:778	XPS	776:778	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	4	50	theme	photoelectron	748:760	arg1	spectroscopy					762:773	X-ray photoelectron spectroscopy	742:773	X-ray photoelectron spectroscopy (XPS)	742:779	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	6	51	theme	osteoblast-like	1198:1212	arg1	line					1219:1222	MG-63 osteoblast-like cell line	1192:1222	MG-63 osteoblast-like cell line	1192:1222	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	8	52	theme	killing	1579:1585	arg1	mechanism					1587:1595	a contact killing mechanism	1569:1595	a contact killing mechanism	1569:1595	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	7	53	theme	equal	1360:1364	arg1	percentage					1349:1358	a silver surface atomic percentage	1325:1358	a silver surface atomic percentage equal to 0.3%	1325:1372	These studies revealed that a coating showing a silver surface atomic percentage equal to 0.3% can be effectively used as antibacterial system, while providing good viability of osteoblast-like cells after 7 days.
29032456	4	54	theme	X-ray	742:746	arg1	XPS					776:778	XPS	776:778	XPS	776:778	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	4	54	theme	X-ray	742:746	arg1	spectroscopy					762:773	X-ray photoelectron spectroscopy	742:773	X-ray photoelectron spectroscopy (XPS)	742:779	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	2	55	from	techniques	495:504	arg1	substrates					544:553	poly(acrylic acid)-coated titanium substrates	509:553	poly(acrylic acid)-coated titanium substrates	509:553	An antibacterial coating based on chitosan and silver was developed by electrodeposition techniques on poly(acrylic acid)-coated titanium substrates.
29032456	7	56	theme	good	1439:1442	arg1	viability					1444:1452	good viability	1439:1452	good viability of osteoblast-like cells	1439:1477	These studies revealed that a coating showing a silver surface atomic percentage equal to 0.3% can be effectively used as antibacterial system, while providing good viability of osteoblast-like cells after 7 days.
29032456	4	57	theme	contact	828:834	arg1	mode					836:839	intermittent contact mode	815:839	intermittent contact mode (ICAFM)	815:847	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	4	57	theme	contact	828:834	arg1	ICAFM					842:846	ICAFM	842:846	ICAFM	842:846	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	3	58	theme	silver	644:649	arg1	incorporation					651:663	a stable and scalable silver incorporation	622:663	a stable and scalable silver incorporation	622:663	When a silver salt was added during the chitosan deposition step, a stable and scalable silver incorporation was achieved.
29032456	6	59	theme	aeruginosa	1245:1254	arg1	strains					1256:1262	S. aureus and P. aeruginosa strains	1228:1262	S. aureus and P. aeruginosa strains	1228:1262	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	7	60	theme	antibacterial	1401:1413	arg1	coating					1309:1315	a coating	1307:1315	a coating showing a silver surface atomic percentage equal to 0.3%	1307:1372	These studies revealed that a coating showing a silver surface atomic percentage equal to 0.3% can be effectively used as antibacterial system, while providing good viability of osteoblast-like cells after 7 days.
29032456	7	60	theme	antibacterial	1401:1413	arg1	system					1415:1420	antibacterial system	1401:1420	antibacterial system	1401:1420	These studies revealed that a coating showing a silver surface atomic percentage equal to 0.3% can be effectively used as antibacterial system, while providing good viability of osteoblast-like cells after 7 days.
29032456	5	61	theme	mass	998:1001	arg1	ICP-MS					1017:1022	ICP-MS	1017:1022	ICP-MS	1017:1022	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	5	61	theme	mass	998:1001	arg1	spectrometry					1003:1014	inductively coupled plasma mass spectrometry	971:1014	inductively coupled plasma mass spectrometry (ICP-MS)	971:1023	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	6	62	theme	aureus	1231:1236	arg1	strains					1256:1262	S. aureus and P. aeruginosa strains	1228:1262	S. aureus and P. aeruginosa strains	1228:1262	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	4	63	theme	intermittent	815:826	arg1	mode					836:839	intermittent contact mode	815:839	intermittent contact mode (ICAFM)	815:847	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	4	63	theme	intermittent	815:826	arg1	ICAFM					842:846	ICAFM	842:846	ICAFM	842:846	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	5	64	theme	silver	907:912	arg1	silver					907:912	silver	907:912	silver	907:912	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	5	64	theme	silver	907:912	arg1	amount					897:902	The amount	893:902	The amount of silver released from the coating up to 21 days	893:952	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	1	65	theme	implant	241:247	arg1	surfaces					249:256	implant surfaces	241:256	implant surfaces	241:256	The present work focuses on the idea to prevent and/or inhibit the colonization of implant surfaces by microbial pathogens responsible for post-operative infections, adjusting antimicrobial properties of the implant surface prior to its insertion.
29032456	2	66	theme	titanium	535:542	arg1	substrates					544:553	poly(acrylic acid)-coated titanium substrates	509:553	poly(acrylic acid)-coated titanium substrates	509:553	An antibacterial coating based on chitosan and silver was developed by electrodeposition techniques on poly(acrylic acid)-coated titanium substrates.
29032456	5	67	theme	plasma	991:996	arg1	ICP-MS					1017:1022	ICP-MS	1017:1022	ICP-MS	1017:1022	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	5	67	theme	plasma	991:996	arg1	spectrometry					1003:1014	inductively coupled plasma mass spectrometry	971:1014	inductively coupled plasma mass spectrometry (ICP-MS)	971:1023	The amount of silver released from the coating up to 21 days was evaluated by inductively coupled plasma mass spectrometry (ICP-MS).
29032456	1	68	theme	present	162:168	arg1	work					170:173	The present work	158:173	The present work	158:173	The present work focuses on the idea to prevent and/or inhibit the colonization of implant surfaces by microbial pathogens responsible for post-operative infections, adjusting antimicrobial properties of the implant surface prior to its insertion.
29032456	6	69	theme	MG-63	1192:1196	arg1	line					1219:1222	MG-63 osteoblast-like cell line	1192:1222	MG-63 osteoblast-like cell line	1192:1222	The capability of the proposed coating to interact in vitro with the biological environment in terms of compatibility and antibacterial properties was assessed using MG-63 osteoblast-like cell line and S. aureus and P. aeruginosa strains, respectively.
29032456	1	70	theme	surfaces	249:256	arg1	colonization					225:236	the colonization	221:236	the colonization of implant surfaces by microbial pathogens responsible for post-operative infections	221:321	The present work focuses on the idea to prevent and/or inhibit the colonization of implant surfaces by microbial pathogens responsible for post-operative infections, adjusting antimicrobial properties of the implant surface prior to its insertion.
29032456	2	71	theme	-coated	527:533	arg1	substrates					544:553	poly(acrylic acid)-coated titanium substrates	509:553	poly(acrylic acid)-coated titanium substrates	509:553	An antibacterial coating based on chitosan and silver was developed by electrodeposition techniques on poly(acrylic acid)-coated titanium substrates.
29032456	1	72	theme	implant	366:372	arg1	surface					374:380	the implant surface	362:380	the implant surface prior to its insertion	362:403	The present work focuses on the idea to prevent and/or inhibit the colonization of implant surfaces by microbial pathogens responsible for post-operative infections, adjusting antimicrobial properties of the implant surface prior to its insertion.
29032456	3	73	theme	scalable	635:642	arg1	incorporation					651:663	a stable and scalable silver incorporation	622:663	a stable and scalable silver incorporation	622:663	When a silver salt was added during the chitosan deposition step, a stable and scalable silver incorporation was achieved.
29032456	4	74	theme	coating	719:725	arg1	composition					700:710	The physico-chemical composition	679:710	The physico-chemical composition of the coating	679:725	The physico-chemical composition of the coating was studied by X-ray photoelectron spectroscopy (XPS), while atomic force microscopy in intermittent contact mode (ICAFM) was used to explore the coating morphology.
29032456	8	75	theme	coating	1541:1547	arg1	effectiveness					1511:1523	The antibacterial effectiveness	1493:1523	The antibacterial effectiveness of the prepared coating	1493:1547	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	8	76	theme	silver	1632:1637	arg1	concentration					1615:1627	the low concentration	1607:1627	the low concentration of silver released (below 0.1 ppm up to 21 days)	1607:1676	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
29032456	8	76	theme	silver	1632:1637	arg1	0.1 ppm					1655:1661	0.1 ppm	1655:1661	0.1 ppm	1655:1661	The antibacterial effectiveness of the prepared coating is mainly driven by a contact killing mechanism, although the low concentration of silver released (below 0.1 ppm up to 21 days) is enough to inhibit bacterial growth, advantaging MG-63 cells in the race for the surface.
27890416	7	0	from	small	1369:1373	arg1	study					1341:1345	our study	1337:1345	our study	1337:1345	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	7	1	theme	summer	1351:1356	arg1	small					1369:1373	small	1369:1373	small	1369:1373	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	7	1	theme	summer	1351:1356	arg1	signal					1358:1363	the summer signal	1347:1363	the summer signal	1347:1363	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	0	2	theme	Alps	143:146	arg1	valley					120:125	a high-altitude glacial valley	96:125	a high-altitude glacial valley of the European Alps	96:146	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.
27890416	5	3	dep	August	926:931	arg1	δ18O					947:950	precipitation δ18O	933:950	precipitation δ18O	933:950	The tree-ring cellulose δ18O values were also found to be influenced by the August precipitation δ18O and mean temperature.
27890416	5	3	dep	August	926:931	arg1	temperature					961:971	mean temperature	956:971	mean temperature	956:971	The tree-ring cellulose δ18O values were also found to be influenced by the August precipitation δ18O and mean temperature.
27890416	1	4	theme	tree-ring	227:235	arg1	widths					237:242	tree-ring widths	227:242	tree-ring widths from European larch (Larix decidua)	227:278	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	4	theme	tree-ring	227:235	arg1	decidua					271:277	Larix decidua	265:277	Larix decidua	265:277	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	8	5	theme	soil	1494:1497	arg1	characteristics					1499:1513	soil characteristics	1494:1513	soil characteristics	1494:1513	Site topography, geomorphology and soil characteristics in particular influence the stable isotope signal in tree-ring cellulose.
27890416	3	6	theme	snow-melt	635:643	arg1	water					645:649	the snow-melt water	631:649	the snow-melt water of the previous winter	631:672	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	3	7	theme	tree-ring	486:494	arg1	cellulose					496:504	tree-ring cellulose	486:504	tree-ring cellulose	486:504	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	8	8	theme	tree-ring	1568:1576	arg1	cellulose					1578:1586	tree-ring cellulose	1568:1586	tree-ring cellulose	1568:1586	Site topography, geomorphology and soil characteristics in particular influence the stable isotope signal in tree-ring cellulose.
27890416	4	9	theme	slope	739:743	arg1	streams					745:751	the slope streams	735:751	the slope streams	735:751	This water, which also comes from the slope streams and from the underground flow of nearby steep slopes, infiltrates the soil in the valley bottom.
27890416	6	10	theme	δ18O	1120:1123	arg1	record					1125:1130	the winter precipitation δ18O record	1095:1130	the winter precipitation δ18O record	1095:1130	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	6	11	from	composition	1198:1208	arg1	region					1252:1257	the study region	1242:1257	the study region	1242:1257	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	7	12	theme	tree-ring	1278:1286	arg1	studies					1288:1294	most other tree-ring studies	1267:1294	most other tree-ring studies that focus on growing season signals	1267:1331	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	3	13	theme	previous	658:665	arg1	winter					667:672	the previous winter	654:672	the previous winter	654:672	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	6	14	theme	winter	1099:1104	arg1	record					1125:1130	the winter precipitation δ18O record	1095:1130	the winter precipitation δ18O record	1095:1130	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	7	15	theme	most	1267:1270	arg1	studies					1288:1294	most other tree-ring studies	1267:1294	most other tree-ring studies that focus on growing season signals	1267:1331	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	0	16	theme	glacial	112:118	arg1	valley					120:125	a high-altitude glacial valley	96:125	a high-altitude glacial valley of the European Alps	96:146	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.
27890416	4	17	from	soil	823:826	arg1	bottom					842:847	the valley bottom	831:847	the valley bottom	831:847	This water, which also comes from the slope streams and from the underground flow of nearby steep slopes, infiltrates the soil in the valley bottom.
27890416	1	18	theme	stable	191:196	arg1	δ18O					217:220	δ18O	217:220	δ18O	217:220	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	18	theme	stable	191:196	arg1	δ13C					208:211	δ13C	208:211	δ13C	208:211	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	18	theme	stable	191:196	arg1	isotopes					198:205	cellulose stable isotopes	181:205	cellulose stable isotopes (δ13C and δ18O)	181:221	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	3	19	theme	growing	685:691	arg1	season					693:698	the growing season	681:698	the growing season	681:698	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	6	20	theme	isotopic	1189:1196	arg1	composition					1198:1208	the δ18O isotopic composition	1180:1208	the δ18O isotopic composition of past winter precipitation in the study region	1180:1257	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	5	21	theme	mean	956:959	arg1	temperature					961:971	mean temperature	956:971	mean temperature	956:971	The tree-ring cellulose δ18O values were also found to be influenced by the August precipitation δ18O and mean temperature.
27890416	3	22	dep	snowfall	558:565	arg1	November					568:575	November	568:575	November	568:575	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	4	23	theme	nearby	786:791	arg1	slopes					799:804	nearby steep slopes	786:804	nearby steep slopes	786:804	This water, which also comes from the slope streams and from the underground flow of nearby steep slopes, infiltrates the soil in the valley bottom.
27890416	6	24	theme	variance	1083:1090	arg1	%					1074:1074	up to 34%	1066:1074	up to 34% of the variance in the winter precipitation δ18O record	1066:1130	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	6	24	theme	variance	1083:1090	arg1	variance					1083:1090	the variance	1079:1090	the variance in the winter precipitation δ18O record	1079:1130	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	6	25	dep	34	1072:1073	arg1	to					1069:1070	to	1069:1070	to	1069:1070	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	0	26	theme	European	134:141	arg1	Alps					143:146	the European Alps	130:146	the European Alps	130:146	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.
27890416	4	27	theme	slopes	799:804	arg1	flow					778:781	the underground flow	762:781	the underground flow of nearby steep slopes	762:804	This water, which also comes from the slope streams and from the underground flow of nearby steep slopes, infiltrates the soil in the valley bottom.
27890416	3	28	theme	snowfall	558:565	arg1	δ18O					543:546	the δ18O	539:546	the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season	539:698	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	1	29	from	chronologies	165:176	arg1	2190ma					305:310	a high-altitude site (2190ma	283:310	a high-altitude site (2190ma	283:310	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	29	from	chronologies	165:176	arg1	larch					258:262	European larch	249:262	European larch	249:262	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	7	30	theme	glacial	1443:1449	arg1	valley					1451:1456	the glacial valley	1439:1456	the glacial valley	1439:1456	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	6	31	from	record	1125:1130	arg1	%					1074:1074	up to 34%	1066:1074	up to 34% of the variance in the winter precipitation δ18O record	1066:1130	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	6	31	from	record	1125:1130	arg1	variance					1083:1090	the variance	1079:1090	the variance in the winter precipitation δ18O record	1079:1130	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	1	32	theme	high-altitude	285:297	arg1	2190ma					305:310	a high-altitude site (2190ma	283:310	a high-altitude site (2190ma	283:310	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	2	33	theme	meteorological	418:431	arg1	variables					433:441	monthly meteorological variables	410:441	monthly meteorological variables	410:441	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	6	34	theme	study	1246:1250	arg1	region					1252:1257	the study region	1242:1257	the study region	1242:1257	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	7	35	theme	special	1417:1423	arg1	conditions					1425:1434	the special conditions	1413:1434	the special conditions of the glacial valley	1413:1456	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	2	36	dep	s.l.	312:315	arg1	bottom					325:330	the bottom	321:330	the bottom of a glacial valley in the Italian Alps	321:370	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	6	37	from	%	1074:1074	arg1	record					1125:1130	the winter precipitation δ18O record	1095:1130	the winter precipitation δ18O record	1095:1130	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	0	38	theme	snow	88:91	arg1	signature					68:76	the isotopic signature	55:76	the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps	55:146	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.
27890416	2	39	theme	precipitation	452:464	arg1	values					466:471	δ18O precipitation values	447:471	δ18O precipitation values	447:471	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	6	40	theme	winter	1218:1223	arg1	precipitation					1225:1237	past winter precipitation	1213:1237	past winter precipitation	1213:1237	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	5	41	theme	cellulose	864:872	arg1	values					879:884	The tree-ring cellulose δ18O values	850:884	The tree-ring cellulose δ18O values	850:884	The tree-ring cellulose δ18O values were also found to be influenced by the August precipitation δ18O and mean temperature.
27890416	2	42	from	dependence	396:405	arg1	variables					433:441	monthly meteorological variables	410:441	monthly meteorological variables	410:441	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	2	42	from	dependence	396:405	arg1	values					466:471	δ18O precipitation values	447:471	δ18O precipitation values	447:471	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	8	43	from	signal	1558:1563	arg1	cellulose					1578:1586	tree-ring cellulose	1568:1586	tree-ring cellulose	1568:1586	Site topography, geomorphology and soil characteristics in particular influence the stable isotope signal in tree-ring cellulose.
27890416	0	44	theme	Larix	0:4	arg1	decidua					6:12	Larix decidua	0:12	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.	0:147	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.
27890416	1	45	theme	European	249:256	arg1	larch					258:262	European larch	249:262	European larch	249:262	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	2	46	theme	valley	345:350	arg1	bottom					325:330	the bottom	321:330	the bottom of a glacial valley in the Italian Alps	321:370	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	2	47	from	bottom	325:330	arg1	Alps					367:370	the Italian Alps	355:370	the Italian Alps	355:370	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	0	48	theme	isotopic	59:66	arg1	signature					68:76	the isotopic signature	55:76	the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps	55:146	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.
27890416	1	49	dep	isotopes	198:205	arg1	δ18O					217:220	δ18O	217:220	δ18O	217:220	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	49	dep	isotopes	198:205	arg1	δ13C					208:211	δ13C	208:211	δ13C	208:211	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	49	dep	isotopes	198:205	arg1	isotopes					198:205	cellulose stable isotopes	181:205	cellulose stable isotopes (δ13C and δ18O)	181:221	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	2	50	from	valley	345:350	arg1	Alps					367:370	the Italian Alps	355:370	the Italian Alps	355:370	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	6	51	theme	associated	978:987	arg1	model					1000:1004	The associated regression model	974:1004	The associated regression model	974:1004	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	8	52	theme	Site	1459:1462	arg1	topography					1464:1473	Site topography	1459:1473	Site topography	1459:1473	Site topography, geomorphology and soil characteristics in particular influence the stable isotope signal in tree-ring cellulose.
27890416	3	53	theme	larch	608:612	arg1	trees					614:618	larch trees	608:618	larch trees	608:618	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	8	54	theme	isotope	1550:1556	arg1	signal					1558:1563	the stable isotope signal	1539:1563	the stable isotope signal in tree-ring cellulose	1539:1586	Site topography, geomorphology and soil characteristics in particular influence the stable isotope signal in tree-ring cellulose.
27890416	1	55	theme	widths	237:242	arg1	chronologies					165:176	the chronologies	161:176	the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma	161:310	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	4	56	theme	underground	766:776	arg1	flow					778:781	the underground flow	762:781	the underground flow of nearby steep slopes	762:804	This water, which also comes from the slope streams and from the underground flow of nearby steep slopes, infiltrates the soil in the valley bottom.
27890416	7	57	theme	growing	1310:1316	arg1	signals					1325:1331	growing season signals	1310:1331	growing season signals	1310:1331	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	7	58	theme	season	1318:1323	arg1	signals					1325:1331	growing season signals	1310:1331	growing season signals	1310:1331	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	6	59	theme	tree	1046:1049	arg1	rings					1051:1055	the tree rings	1042:1055	the tree rings	1042:1055	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	6	60	theme	precipitation	1106:1118	arg1	record					1125:1130	the winter precipitation δ18O record	1095:1130	the winter precipitation δ18O record	1095:1130	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	1	61	from	larch	258:262	arg1	chronologies					165:176	the chronologies	161:176	the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma	161:310	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	61	from	larch	258:262	arg1	δ13C					208:211	δ13C	208:211	δ13C	208:211	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	61	from	larch	258:262	arg1	δ18O					217:220	δ18O	217:220	δ18O	217:220	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	61	from	larch	258:262	arg1	isotopes					198:205	cellulose stable isotopes	181:205	cellulose stable isotopes (δ13C and δ18O)	181:221	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	61	from	larch	258:262	arg1	widths					237:242	tree-ring widths	227:242	tree-ring widths from European larch (Larix decidua)	227:278	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	61	from	larch	258:262	arg1	decidua					271:277	Larix decidua	265:277	Larix decidua	265:277	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	3	62	theme	winter	667:672	arg1	water					645:649	the snow-melt water	631:649	the snow-melt water of the previous winter	631:672	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	6	63	theme	past	1213:1216	arg1	precipitation					1225:1237	past winter precipitation	1213:1237	past winter precipitation	1213:1237	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	3	64	theme	cellulose	496:504	arg1	δ18O					478:481	The δ18O	474:481	The δ18O of tree-ring cellulose	474:504	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	1	65	theme	cellulose	181:189	arg1	δ18O					217:220	δ18O	217:220	δ18O	217:220	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	65	theme	cellulose	181:189	arg1	δ13C					208:211	δ13C	208:211	δ13C	208:211	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	65	theme	cellulose	181:189	arg1	isotopes					198:205	cellulose stable isotopes	181:205	cellulose stable isotopes (δ13C and δ18O)	181:221	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	6	66	theme	δ18O	1021:1024	arg1	chronology					1026:1035	the δ18O chronology	1017:1035	the δ18O chronology from the tree rings	1017:1055	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	1	67	theme	isotopes	198:205	arg1	chronologies					165:176	the chronologies	161:176	the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma	161:310	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	7	68	from	study	1341:1345	arg1	small					1369:1373	small	1369:1373	small	1369:1373	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	7	68	from	study	1341:1345	arg1	signal					1358:1363	the summer signal	1347:1363	the summer signal	1347:1363	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	0	69	from	signature	68:76	arg1	valley					120:125	a high-altitude glacial valley	96:125	a high-altitude glacial valley of the European Alps	96:146	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.
27890416	6	70	theme	δ18O	1184:1187	arg1	composition					1198:1208	the δ18O isotopic composition	1180:1208	the δ18O isotopic composition of past winter precipitation in the study region	1180:1257	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	7	71	theme	valley	1451:1456	arg1	conditions					1425:1434	the special conditions	1413:1434	the special conditions of the glacial valley	1413:1456	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	8	72	from	topography	1464:1473	arg1	particular					1518:1527	particular	1518:1527	particular	1518:1527	Site topography, geomorphology and soil characteristics in particular influence the stable isotope signal in tree-ring cellulose.
27890416	6	73	from	variance	1083:1090	arg1	record					1125:1130	the winter precipitation δ18O record	1095:1130	the winter precipitation δ18O record	1095:1130	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	8	74	from	geomorphology	1476:1488	arg1	particular					1518:1527	particular	1518:1527	particular	1518:1527	Site topography, geomorphology and soil characteristics in particular influence the stable isotope signal in tree-ring cellulose.
27890416	3	75	theme	winter	551:556	arg1	snowfall					558:565	winter snowfall	551:565	winter snowfall (November to March)	551:585	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	4	76	theme	steep	793:797	arg1	slopes					799:804	nearby steep slopes	786:804	nearby steep slopes	786:804	This water, which also comes from the slope streams and from the underground flow of nearby steep slopes, infiltrates the soil in the valley bottom.
27890416	5	77	theme	precipitation	933:945	arg1	δ18O					947:950	precipitation δ18O	933:950	precipitation δ18O	933:950	The tree-ring cellulose δ18O values were also found to be influenced by the August precipitation δ18O and mean temperature.
27890416	2	78	from	Alps	367:370	arg1	bottom					325:330	the bottom	321:330	the bottom of a glacial valley in the Italian Alps	321:370	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	4	79	theme	valley	835:840	arg1	bottom					842:847	the valley bottom	831:847	the valley bottom	831:847	This water, which also comes from the slope streams and from the underground flow of nearby steep slopes, infiltrates the soil in the valley bottom.
27890416	0	80	theme	winter	81:86	arg1	snow					88:91	winter snow	81:91	winter snow	81:91	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.
27890416	1	81	theme	site	299:302	arg1	2190ma					305:310	a high-altitude site (2190ma	283:310	a high-altitude site (2190ma	283:310	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	2	82	theme	monthly	410:416	arg1	variables					433:441	monthly meteorological variables	410:441	monthly meteorological variables	410:441	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	7	83	theme	other	1272:1276	arg1	studies					1288:1294	most other tree-ring studies	1267:1294	most other tree-ring studies that focus on growing season signals	1267:1331	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	6	84	from	rings	1051:1055	arg1	chronology					1026:1035	the δ18O chronology	1017:1035	the δ18O chronology from the tree rings	1017:1055	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	0	85	theme	high-altitude	98:110	arg1	valley					120:125	a high-altitude glacial valley	96:125	a high-altitude glacial valley of the European Alps	96:146	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.
27890416	5	86	theme	tree-ring	854:862	arg1	values					879:884	The tree-ring cellulose δ18O values	850:884	The tree-ring cellulose δ18O values	850:884	The tree-ring cellulose δ18O values were also found to be influenced by the August precipitation δ18O and mean temperature.
27890416	2	87	theme	δ18O	447:450	arg1	values					466:471	δ18O precipitation values	447:471	δ18O precipitation values	447:471	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	8	88	from	characteristics	1499:1513	arg1	particular					1518:1527	particular	1518:1527	particular	1518:1527	Site topography, geomorphology and soil characteristics in particular influence the stable isotope signal in tree-ring cellulose.
27890416	6	89	theme	precipitation	1225:1237	arg1	composition					1198:1208	the δ18O isotopic composition	1180:1208	the δ18O isotopic composition of past winter precipitation in the study region	1180:1257	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	0	90	theme	tree-ring	19:27	arg1	cellulose					29:37	tree-ring cellulose	19:37	tree-ring cellulose	19:37	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.
27890416	2	91	theme	Italian	359:365	arg1	Alps					367:370	the Italian Alps	355:370	the Italian Alps	355:370	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	1	92	theme	Larix	265:269	arg1	widths					237:242	tree-ring widths	227:242	tree-ring widths from European larch (Larix decidua)	227:278	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	1	92	theme	Larix	265:269	arg1	decidua					271:277	Larix decidua	265:277	Larix decidua	265:277	We analyzed the chronologies of cellulose stable isotopes (δ13C and δ18O) and tree-ring widths from European larch (Larix decidua) in a high-altitude site (2190ma.
27890416	7	93	theme	dominant	1397:1404	arg1	signal					1390:1395	the winter signal	1379:1395	the winter signal dominant due to the special conditions of the glacial valley	1379:1456	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	2	94	theme	glacial	337:343	arg1	valley					345:350	a glacial valley	335:350	a glacial valley in the Italian Alps	335:370	s.l.) at the bottom of a glacial valley in the Italian Alps, and investigated their dependence on monthly meteorological variables and δ18O precipitation values.
27890416	7	95	theme	winter	1383:1388	arg1	signal					1390:1395	the winter signal	1379:1395	the winter signal dominant due to the special conditions of the glacial valley	1379:1456	Unlike most other tree-ring studies that focus on growing season signals, in our study the summer signal was small and the winter signal dominant due to the special conditions of the glacial valley.
27890416	5	96	theme	δ18O	874:877	arg1	values					879:884	The tree-ring cellulose δ18O values	850:884	The tree-ring cellulose δ18O values	850:884	The tree-ring cellulose δ18O values were also found to be influenced by the August precipitation δ18O and mean temperature.
27890416	3	97	dep	November	568:575	arg1	to					577:578	to	577:578	to	577:578	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	3	97	dep	November	568:575	arg1	March					580:584	March	580:584	March	580:584	The δ18O of tree-ring cellulose appears to be strongly driven by the δ18O of winter snowfall (November to March), which suggests that larch trees mostly use the snow-melt water of the previous winter during the growing season.
27890416	0	98	dep	decidua	6:12	arg1	reflects					46:53	reflects	46:53	reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps	46:146	Larix decidua δ18O tree-ring cellulose mainly reflects the isotopic signature of winter snow in a high-altitude glacial valley of the European Alps.
27890416	6	99	theme	regression	989:998	arg1	model					1000:1004	The associated regression model	974:1004	The associated regression model	974:1004	The associated regression model shows that the δ18O chronology from the tree rings explains up to 34% of the variance in the winter precipitation δ18O record, demonstrating the potential for reconstructing the δ18O isotopic composition of past winter precipitation in the study region.
27890416	8	100	theme	stable	1543:1548	arg1	signal					1558:1563	the stable isotope signal	1539:1563	the stable isotope signal in tree-ring cellulose	1539:1586	Site topography, geomorphology and soil characteristics in particular influence the stable isotope signal in tree-ring cellulose.
28747495	9	0	theme	HIV	1872:1874	arg1	glycoprotein					1851:1862	the gp120 envelope glycoprotein	1832:1862	the gp120 envelope glycoprotein of both HIV and SIV	1832:1882	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV is N-linked carbohydrate.
28747495	3	1	theme	amino	654:658	arg1	substitution					665:676	amino acid substitution	654:676	amino acid substitution	654:676	We found that this Thr was critical for the association of gp120 with the virion and that amino acid substitution increased the amount of dissociated gp120 in the cell culture supernatant.
28747495	7	2	theme	HIV	1445:1447	arg1	cycle					1436:1440	the natural replication cycle	1412:1440	the natural replication cycle of HIV and SIV	1412:1455	Our data indicate that O-glycosylation is not necessary for the natural replication cycle of HIV and SIV.
28747495	9	3	theme	SIV	1880:1882	arg1	glycoprotein					1851:1862	the gp120 envelope glycoprotein	1832:1862	the gp120 envelope glycoprotein of both HIV and SIV	1832:1882	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV is N-linked carbohydrate.
28747495	8	4	theme	threonine	1643:1651	arg1	residue					1653:1659	the threonine residue	1639:1659	the threonine residue in question	1639:1671	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	10	5	theme	contributions	1921:1933	arg1	One					1910:1912	One	1910:1912	One	1910:1912	One of the contributions of this N-linked carbohydrate is to shield conserved peptide sequences from recognition by humoral immunity.
28747495	10	5	theme	contributions	1921:1933	arg1	contributions					1921:1933	the contributions	1917:1933	the contributions of this N-linked carbohydrate	1917:1963	One of the contributions of this N-linked carbohydrate is to shield conserved peptide sequences from recognition by humoral immunity.
28747495	6	6	theme	normal	1273:1278	arg1	levels					1280:1285	normal levels	1273:1285	normal levels of infectivity	1273:1300	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	1	7	theme	immunodeficiency	261:276	arg1	SIV					285:287	SIV	285:287	SIV	285:287	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	1	7	theme	immunodeficiency	261:276	arg1	virus					278:282	simian immunodeficiency virus	254:282	simian immunodeficiency virus (SIV)	254:288	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	11	8	theme	SIV	2127:2129	arg1	isolates					2107:2114	primary isolates	2099:2114	primary isolates of HIV and SIV	2099:2129	This N-linked glycosylation is one of the reasons that primary isolates of HIV and SIV are so heavily resistant to antibody-mediated neutralization.
28747495	6	9	theme	ability	1171:1177	arg1	devoid					1157:1162	devoid	1157:1162	devoid	1157:1162	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	9	10	theme	N-linked	1887:1894	arg1	carbohydrate					1896:1907	N-linked carbohydrate	1887:1907	N-linked carbohydrate	1887:1907	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV is N-linked carbohydrate.
28747495	6	11	theme	virions	1261:1267	arg1	levels					1280:1285	normal levels	1273:1285	normal levels of infectivity	1273:1300	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	6	11	theme	virions	1261:1267	arg1	levels					1251:1256	normal levels	1244:1256	normal levels of virions	1244:1267	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	8	12	from	repertoire	1514:1523	arg1	cells					1574:1578	natural target cells	1559:1578	natural target cells for HIV and SIV	1559:1594	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	8	13	theme	virions	1718:1724	arg1	infectivity					1703:1713	the infectivity	1699:1713	the infectivity of virions	1699:1724	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	9	14	link	N-linked	1887:1894	arg1	carbohydrate					1896:1907	N-linked carbohydrate	1887:1907	N-linked carbohydrate	1887:1907	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV is N-linked carbohydrate.
28747495	11	15	theme	HIV	2119:2121	arg1	isolates					2107:2114	primary isolates	2099:2114	primary isolates of HIV and SIV	2099:2129	This N-linked glycosylation is one of the reasons that primary isolates of HIV and SIV are so heavily resistant to antibody-mediated neutralization.
28747495	7	16	theme	natural	1416:1422	arg1	cycle					1436:1440	the natural replication cycle	1412:1440	the natural replication cycle of HIV and SIV	1412:1455	Our data indicate that O-glycosylation is not necessary for the natural replication cycle of HIV and SIV.
28747495	9	17	dep	IMPORTANCE	1788:1797	arg1	%					1815:1815	Approximately 50%	1799:1815	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV	1788:1882	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV is N-linked carbohydrate.
28747495	3	18	from	amount	692:697	arg1	supernatant					740:750	the cell culture supernatant	723:750	the cell culture supernatant	723:750	We found that this Thr was critical for the association of gp120 with the virion and that amino acid substitution increased the amount of dissociated gp120 in the cell culture supernatant.
28747495	10	19	theme	carbohydrate	1952:1963	arg1	contributions					1921:1933	the contributions	1917:1933	the contributions of this N-linked carbohydrate	1917:1963	One of the contributions of this N-linked carbohydrate is to shield conserved peptide sequences from recognition by humoral immunity.
28747495	6	20	theme	levels	1251:1256	arg1	production					1230:1239	production	1230:1239	production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate	1230:1349	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	3	21	with	association	608:618	arg1	virion					638:643	the virion	634:643	the virion	634:643	We found that this Thr was critical for the association of gp120 with the virion and that amino acid substitution increased the amount of dissociated gp120 in the cell culture supernatant.
28747495	3	22	theme	gp120	714:718	arg1	amount					692:697	the amount	688:697	the amount of dissociated gp120 in the cell culture supernatant	688:750	We found that this Thr was critical for the association of gp120 with the virion and that amino acid substitution increased the amount of dissociated gp120 in the cell culture supernatant.
28747495	3	22	theme	gp120	714:718	arg1	gp120					714:718	dissociated gp120	702:718	dissociated gp120	702:718	We found that this Thr was critical for the association of gp120 with the virion and that amino acid substitution increased the amount of dissociated gp120 in the cell culture supernatant.
28747495	6	23	theme	carbohydrate	1338:1349	arg1	absence					1318:1324	the complete absence	1305:1324	the complete absence of O-linked carbohydrate	1305:1349	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	1	24	theme	gp120	206:210	arg1	terminus					194:201	the C terminus	188:201	the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV)	188:288	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	6	25	theme	normal	1244:1249	arg1	levels					1251:1256	normal levels	1244:1256	normal levels of virions	1244:1267	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	10	26	theme	conserved	1978:1986	arg1	sequences					1996:2004	conserved peptide sequences	1978:2004	conserved peptide sequences	1978:2004	One of the contributions of this N-linked carbohydrate is to shield conserved peptide sequences from recognition by humoral immunity.
28747495	0	27	theme	O-Glycosylation	137:151	arg1	Absence					115:121	the Complete Absence	102:121	the Complete Absence of Mucin-Type O-Glycosylation	102:151	Human Immunodeficiency Virus and Simian Immunodeficiency Virus Maintain High Levels of Infectivity in the Complete Absence of Mucin-Type O-Glycosylation.
28747495	1	28	theme	human	215:219	arg1	HIV					245:247	HIV	245:247	HIV	245:247	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	1	28	theme	human	215:219	arg1	virus					238:242	human immunodeficiency virus	215:242	human immunodeficiency virus (HIV)	215:248	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	12	29	from	glycosylation	2255:2267	arg1	contribution					2228:2239	any potential contribution	2214:2239	any potential contribution from O-linked glycosylation	2214:2267	Much less studied is any potential contribution from O-linked glycosylation.
28747495	3	30	theme	culture	732:738	arg1	supernatant					740:750	the cell culture supernatant	723:750	the cell culture supernatant	723:750	We found that this Thr was critical for the association of gp120 with the virion and that amino acid substitution increased the amount of dissociated gp120 in the cell culture supernatant.
28747495	6	31	theme	complete	1309:1316	arg1	absence					1318:1324	the complete absence	1305:1324	the complete absence of O-linked carbohydrate	1305:1349	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	8	32	from	O-glycosylation	1620:1634	arg1	question					1664:1671	question	1664:1671	question	1664:1671	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	5	33	theme	infectivity	1048:1058	arg1	enhancements					1060:1071	These infectivity enhancements	1042:1071	These infectivity enhancements	1042:1071	These infectivity enhancements were not observed when GalNAcT3 was used.
28747495	8	34	theme	O-glycosylation	1772:1786	arg1	absence					1756:1762	the absence	1752:1762	the absence of such O-glycosylation	1752:1786	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	11	35	theme	primary	2099:2105	arg1	isolates					2107:2114	primary isolates	2099:2114	primary isolates of HIV and SIV	2099:2129	This N-linked glycosylation is one of the reasons that primary isolates of HIV and SIV are so heavily resistant to antibody-mediated neutralization.
28747495	0	36	theme	High	72:75	arg1	Levels					77:82	High Levels	72:82	High Levels of Infectivity	72:97	Human Immunodeficiency Virus and Simian Immunodeficiency Virus Maintain High Levels of Infectivity in the Complete Absence of Mucin-Type O-Glycosylation.
28747495	15	37	link	O-linked	2767:2774	arg1	attachment					2789:2798	such O-linked carbohydrate attachment	2762:2798	such O-linked carbohydrate attachment	2762:2798	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	2	38	theme	amino	480:484	arg1	acid					486:489	the other amino acid	470:489	the other amino acid that can accept O-glycosylation	470:521	When this highly conserved Thr residue was substituted with anything other than serine (the other amino acid that can accept O-glycosylation), the resulting virus was noninfectious.
28747495	1	39	theme	envelope	332:339	arg1	function					349:356	envelope protein function	332:356	envelope protein function	332:356	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	9	40	gly	glycoprotein	1851:1862	arg1	glycoprotein					1851:1862	the gp120 envelope glycoprotein	1832:1862	the gp120 envelope glycoprotein of both HIV and SIV	1832:1882	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV is N-linked carbohydrate.
28747495	10	41	theme	humoral	2026:2032	arg1	immunity					2034:2041	humoral immunity	2026:2041	humoral immunity	2026:2041	One of the contributions of this N-linked carbohydrate is to shield conserved peptide sequences from recognition by humoral immunity.
28747495	15	42	theme	target	2698:2703	arg1	cells					2705:2709	natural target cells	2690:2709	natural target cells	2690:2709	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	4	43	theme	HEK293T	958:964	arg1	cells					966:970	wild-type HEK293T cells	948:970	wild-type HEK293T cells	948:970	When HIV virions were generated in cells overexpressing polypeptide N-acetylgalactosaminyltransferase 1 (GalNAcT1), viral infectivity was increased 2.5-fold compared to that of virus produced in wild-type HEK293T cells; infectivity was increased 8-fold when the Thr499Ser mutant was used.
28747495	8	44	theme	GalNAc	1528:1533	arg1	transferase					1535:1545	GalNAc transferase	1528:1545	GalNAc transferase isoforms	1528:1554	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	15	45	theme	such	2762:2765	arg1	attachment					2789:2798	such O-linked carbohydrate attachment	2762:2798	such O-linked carbohydrate attachment	2762:2798	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	12	46	theme	O-linked	2246:2253	arg1	glycosylation					2255:2267	O-linked glycosylation	2246:2267	O-linked glycosylation	2246:2267	Much less studied is any potential contribution from O-linked glycosylation.
28747495	0	47	theme	Complete	106:113	arg1	Absence					115:121	the Complete Absence	102:121	the Complete Absence of Mucin-Type O-Glycosylation	102:151	Human Immunodeficiency Virus and Simian Immunodeficiency Virus Maintain High Levels of Infectivity in the Complete Absence of Mucin-Type O-Glycosylation.
28747495	1	48	theme	virion	362:367	arg1	infectivity					369:379	virion infectivity	362:379	virion infectivity	362:379	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	8	49	theme	isoforms	1547:1554	arg1	repertoire					1514:1523	the repertoire	1510:1523	the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo	1510:1602	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	8	49	theme	isoforms	1547:1554	arg1	isoforms					1547:1554	GalNAc transferase isoforms	1528:1554	GalNAc transferase isoforms	1528:1554	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	0	50	theme	Human	0:4	arg1	Virus					23:27	Human Immunodeficiency Virus	0:27	Human Immunodeficiency Virus	0:27	Human Immunodeficiency Virus and Simian Immunodeficiency Virus Maintain High Levels of Infectivity in the Complete Absence of Mucin-Type O-Glycosylation.
28747495	1	51	theme	virus	278:282	arg1	gp120					206:210	gp120	206:210	gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV)	206:288	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	8	52	theme	natural	1559:1565	arg1	cells					1574:1578	natural target cells	1559:1578	natural target cells for HIV and SIV	1559:1594	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	2	53	theme	Thr	409:411	arg1	residue					413:419	this highly conserved Thr residue	387:419	this highly conserved Thr residue	387:419	When this highly conserved Thr residue was substituted with anything other than serine (the other amino acid that can accept O-glycosylation), the resulting virus was noninfectious.
28747495	0	54	theme	Simian	33:38	arg1	Virus					57:61	Simian Immunodeficiency Virus	33:61	Simian Immunodeficiency Virus	33:61	Human Immunodeficiency Virus and Simian Immunodeficiency Virus Maintain High Levels of Infectivity in the Complete Absence of Mucin-Type O-Glycosylation.
28747495	11	55	theme	antibody-mediated	2159:2175	arg1	neutralization					2177:2190	antibody-mediated neutralization	2159:2190	antibody-mediated neutralization	2159:2190	This N-linked glycosylation is one of the reasons that primary isolates of HIV and SIV are so heavily resistant to antibody-mediated neutralization.
28747495	4	56	theme	polypeptide	809:819	arg1	N-acetylgalactosaminyltransferase					821:853	polypeptide N-acetylgalactosaminyltransferase 1	809:855	polypeptide N-acetylgalactosaminyltransferase 1 (GalNAcT1)	809:866	When HIV virions were generated in cells overexpressing polypeptide N-acetylgalactosaminyltransferase 1 (GalNAcT1), viral infectivity was increased 2.5-fold compared to that of virus produced in wild-type HEK293T cells; infectivity was increased 8-fold when the Thr499Ser mutant was used.
28747495	4	56	theme	polypeptide	809:819	arg1	GalNAcT1					858:865	GalNAcT1	858:865	GalNAcT1	858:865	When HIV virions were generated in cells overexpressing polypeptide N-acetylgalactosaminyltransferase 1 (GalNAcT1), viral infectivity was increased 2.5-fold compared to that of virus produced in wild-type HEK293T cells; infectivity was increased 8-fold when the Thr499Ser mutant was used.
28747495	14	57	link	O-linked	2412:2419	arg1	glycosylation					2421:2433	O-linked glycosylation	2412:2433	O-linked glycosylation	2412:2433	Our studies described in this report demonstrate unambiguously that O-linked glycosylation is not necessary for the natural replication cycle of HIV and SIV.
28747495	6	58	theme	knockout	1129:1136	arg1	lines					1143:1147	HEK293T knockout cell lines	1121:1147	HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation	1121:1211	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	6	59	link	O-linked	1329:1336	arg1	carbohydrate					1338:1349	O-linked carbohydrate	1329:1349	O-linked carbohydrate	1329:1349	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	8	60	theme	residue	1653:1659	arg1	O-glycosylation					1620:1634	O-glycosylation	1620:1634	O-glycosylation of the threonine residue in question	1620:1671	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	1	61	theme	immunodeficiency	221:236	arg1	HIV					245:247	HIV	245:247	HIV	245:247	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	1	61	theme	immunodeficiency	221:236	arg1	virus					238:242	human immunodeficiency virus	215:242	human immunodeficiency virus (HIV)	215:248	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	10	62	link	N-linked	1943:1950	arg1	carbohydrate					1952:1963	this N-linked carbohydrate	1938:1963	this N-linked carbohydrate	1938:1963	One of the contributions of this N-linked carbohydrate is to shield conserved peptide sequences from recognition by humoral immunity.
28747495	9	63	theme	glycoprotein	1851:1862	arg1	mass					1824:1827	the mass	1820:1827	the mass of the gp120 envelope glycoprotein of both HIV and SIV	1820:1882	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV is N-linked carbohydrate.
28747495	15	64	theme	GalNAc	2579:2584	arg1	enzymes					2598:2604	numerous GalNAc transferase enzymes	2570:2604	numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment	2570:2651	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	6	65	theme	infectivity	1290:1300	arg1	levels					1280:1285	normal levels	1273:1285	normal levels of infectivity	1273:1300	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	6	65	theme	infectivity	1290:1300	arg1	levels					1251:1256	normal levels	1244:1256	normal levels of virions	1244:1267	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	14	66	theme	replication	2468:2478	arg1	cycle					2480:2484	the natural replication cycle	2456:2484	the natural replication cycle of HIV and SIV	2456:2499	Our studies described in this report demonstrate unambiguously that O-linked glycosylation is not necessary for the natural replication cycle of HIV and SIV.
28747495	14	67	theme	SIV	2497:2499	arg1	cycle					2480:2484	the natural replication cycle	2456:2484	the natural replication cycle of HIV and SIV	2456:2499	Our studies described in this report demonstrate unambiguously that O-linked glycosylation is not necessary for the natural replication cycle of HIV and SIV.
28747495	3	68	theme	acid	660:663	arg1	substitution					665:676	amino acid substitution	654:676	amino acid substitution	654:676	We found that this Thr was critical for the association of gp120 with the virion and that amino acid substitution increased the amount of dissociated gp120 in the cell culture supernatant.
28747495	6	69	theme	levels	1280:1285	arg1	production					1230:1239	production	1230:1239	production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate	1230:1349	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	13	70	from	literature	2274:2283	arg1	topic					2293:2297	this topic	2288:2297	this topic	2288:2297	The literature on this topic to date is somewhat confusing and ambiguous.
28747495	1	71	theme	simian	254:259	arg1	SIV					285:287	SIV	285:287	SIV	285:287	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	1	71	theme	simian	254:259	arg1	virus					278:282	simian immunodeficiency virus	254:282	simian immunodeficiency virus (SIV)	254:288	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	14	72	theme	HIV	2489:2491	arg1	cycle					2480:2484	the natural replication cycle	2456:2484	the natural replication cycle of HIV and SIV	2456:2499	Our studies described in this report demonstrate unambiguously that O-linked glycosylation is not necessary for the natural replication cycle of HIV and SIV.
28747495	4	73	theme	viral	869:873	arg1	infectivity					875:885	viral infectivity	869:885	viral infectivity	869:885	When HIV virions were generated in cells overexpressing polypeptide N-acetylgalactosaminyltransferase 1 (GalNAcT1), viral infectivity was increased 2.5-fold compared to that of virus produced in wild-type HEK293T cells; infectivity was increased 8-fold when the Thr499Ser mutant was used.
28747495	3	74	theme	dissociated	702:712	arg1	gp120					714:718	dissociated gp120	702:718	dissociated gp120	702:718	We found that this Thr was critical for the association of gp120 with the virion and that amino acid substitution increased the amount of dissociated gp120 in the cell culture supernatant.
28747495	15	75	theme	theoretical	2661:2671	arg1	possibility					2673:2683	the theoretical possibility	2657:2683	the theoretical possibility that natural target cells for HIV and SIV in vivo	2657:2733	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	7	76	theme	SIV	1453:1455	arg1	cycle					1436:1440	the natural replication cycle	1412:1440	the natural replication cycle of HIV and SIV	1412:1455	Our data indicate that O-glycosylation is not necessary for the natural replication cycle of HIV and SIV.
28747495	15	77	link	O-linked	2620:2627	arg1	attachment					2642:2651	O-linked carbohydrate attachment	2620:2651	O-linked carbohydrate attachment	2620:2651	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	15	78	theme	carbohydrate	2776:2787	arg1	attachment					2789:2798	such O-linked carbohydrate attachment	2762:2798	such O-linked carbohydrate attachment	2762:2798	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	1	79	theme	C	192:192	arg1	terminus					194:201	the C terminus	188:201	the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV)	188:288	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	7	80	theme	replication	1424:1434	arg1	cycle					1436:1440	the natural replication cycle	1412:1440	the natural replication cycle of HIV and SIV	1412:1455	Our data indicate that O-glycosylation is not necessary for the natural replication cycle of HIV and SIV.
28747495	3	81	theme	cell	727:730	arg1	supernatant					740:750	the cell culture supernatant	723:750	the cell culture supernatant	723:750	We found that this Thr was critical for the association of gp120 with the virion and that amino acid substitution increased the amount of dissociated gp120 in the cell culture supernatant.
28747495	0	82	theme	Mucin-Type	126:135	arg1	O-Glycosylation					137:151	Mucin-Type O-Glycosylation	126:151	Mucin-Type O-Glycosylation	126:151	Human Immunodeficiency Virus and Simian Immunodeficiency Virus Maintain High Levels of Infectivity in the Complete Absence of Mucin-Type O-Glycosylation.
28747495	15	83	theme	carbohydrate	2629:2640	arg1	attachment					2642:2651	O-linked carbohydrate attachment	2620:2651	O-linked carbohydrate attachment	2620:2651	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	6	84	theme	devoid	1157:1162	arg1	lines					1143:1147	HEK293T knockout cell lines	1121:1147	HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation	1121:1211	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	2	85	theme	resulting	529:537	arg1	noninfectious					549:561	noninfectious	549:561	noninfectious	549:561	When this highly conserved Thr residue was substituted with anything other than serine (the other amino acid that can accept O-glycosylation), the resulting virus was noninfectious.
28747495	2	85	theme	resulting	529:537	arg1	virus					539:543	the resulting virus	525:543	the resulting virus	525:543	When this highly conserved Thr residue was substituted with anything other than serine (the other amino acid that can accept O-glycosylation), the resulting virus was noninfectious.
28747495	15	86	theme	O-linked	2620:2627	arg1	attachment					2642:2651	O-linked carbohydrate attachment	2620:2651	O-linked carbohydrate attachment	2620:2651	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	6	87	theme	O-linked	1329:1336	arg1	carbohydrate					1338:1349	O-linked carbohydrate	1329:1349	O-linked carbohydrate	1329:1349	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	9	88	theme	gp120	1836:1840	arg1	glycoprotein					1851:1862	the gp120 envelope glycoprotein	1832:1862	the gp120 envelope glycoprotein of both HIV and SIV	1832:1882	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV is N-linked carbohydrate.
28747495	11	89	theme	N-linked	2049:2056	arg1	glycosylation					2058:2070	This N-linked glycosylation	2044:2070	This N-linked glycosylation	2044:2070	This N-linked glycosylation is one of the reasons that primary isolates of HIV and SIV are so heavily resistant to antibody-mediated neutralization.
28747495	6	90	link	O-linked	1190:1197	arg1	glycosylation					1199:1211	O-linked glycosylation	1190:1211	O-linked glycosylation	1190:1211	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	10	91	theme	N-linked	1943:1950	arg1	carbohydrate					1952:1963	this N-linked carbohydrate	1938:1963	this N-linked carbohydrate	1938:1963	One of the contributions of this N-linked carbohydrate is to shield conserved peptide sequences from recognition by humoral immunity.
28747495	11	92	theme	reasons	2086:2092	arg1	one					2075:2077	one	2075:2077	one	2075:2077	This N-linked glycosylation is one of the reasons that primary isolates of HIV and SIV are so heavily resistant to antibody-mediated neutralization.
28747495	11	92	theme	reasons	2086:2092	arg1	reasons					2086:2092	the reasons	2082:2092	the reasons	2082:2092	This N-linked glycosylation is one of the reasons that primary isolates of HIV and SIV are so heavily resistant to antibody-mediated neutralization.
28747495	6	93	from	production	1230:1239	arg1	absence					1318:1324	the complete absence	1305:1324	the complete absence of O-linked carbohydrate	1305:1349	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	12	94	link	O-linked	2246:2253	arg1	glycosylation					2255:2267	O-linked glycosylation	2246:2267	O-linked glycosylation	2246:2267	Much less studied is any potential contribution from O-linked glycosylation.
28747495	5	95	used	used	1109:1112	arg2	GalNAcT3					1096:1103	GalNAcT3	1096:1103	GalNAcT3	1096:1103	These infectivity enhancements were not observed when GalNAcT3 was used.
28747495	8	96	theme	such	1767:1770	arg1	O-glycosylation					1772:1786	such O-glycosylation	1767:1786	such O-glycosylation	1767:1786	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	15	97	theme	enzymes	2598:2604	arg1	diversity					2557:2565	the diversity	2553:2565	the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment	2553:2651	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	6	98	theme	O-linked	1190:1197	arg1	glycosylation					1199:1211	O-linked glycosylation	1190:1211	O-linked glycosylation	1190:1211	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	10	99	theme	peptide	1988:1994	arg1	sequences					1996:2004	conserved peptide sequences	1978:2004	conserved peptide sequences	1978:2004	One of the contributions of this N-linked carbohydrate is to shield conserved peptide sequences from recognition by humoral immunity.
28747495	0	100	theme	Infectivity	87:97	arg1	Levels					77:82	High Levels	72:82	High Levels of Infectivity	72:97	Human Immunodeficiency Virus and Simian Immunodeficiency Virus Maintain High Levels of Infectivity in the Complete Absence of Mucin-Type O-Glycosylation.
28747495	4	101	used	used	1036:1039	arg2	mutant					1025:1030	the Thr499Ser mutant	1011:1030	the Thr499Ser mutant	1011:1030	When HIV virions were generated in cells overexpressing polypeptide N-acetylgalactosaminyltransferase 1 (GalNAcT1), viral infectivity was increased 2.5-fold compared to that of virus produced in wild-type HEK293T cells; infectivity was increased 8-fold when the Thr499Ser mutant was used.
28747495	2	102	dep	serine	462:467	arg1	acid					486:489	the other amino acid	470:489	the other amino acid that can accept O-glycosylation	470:521	When this highly conserved Thr residue was substituted with anything other than serine (the other amino acid that can accept O-glycosylation), the resulting virus was noninfectious.
28747495	1	103	theme	conserved	163:171	arg1	threonine					173:181	A highly conserved threonine	154:181	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV)	154:288	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	2	104	theme	other	474:478	arg1	acid					486:489	the other amino acid	470:489	the other amino acid that can accept O-glycosylation	470:521	When this highly conserved Thr residue was substituted with anything other than serine (the other amino acid that can accept O-glycosylation), the resulting virus was noninfectious.
28747495	15	105	theme	natural	2690:2696	arg1	cells					2705:2709	natural target cells	2690:2709	natural target cells	2690:2709	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	1	106	theme	protein	341:347	arg1	function					349:356	envelope protein function	332:356	envelope protein function	332:356	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	15	107	theme	O-linked	2767:2774	arg1	attachment					2789:2798	such O-linked carbohydrate attachment	2762:2798	such O-linked carbohydrate attachment	2762:2798	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	8	108	theme	transferase	1535:1545	arg1	isoforms					1547:1554	GalNAc transferase isoforms	1528:1554	GalNAc transferase isoforms	1528:1554	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	12	109	theme	potential	2218:2226	arg1	contribution					2228:2239	any potential contribution	2214:2239	any potential contribution from O-linked glycosylation	2214:2267	Much less studied is any potential contribution from O-linked glycosylation.
28747495	8	110	from	question	1664:1671	arg1	O-glycosylation					1620:1634	O-glycosylation	1620:1634	O-glycosylation of the threonine residue in question	1620:1671	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	4	111	theme	wild-type	948:956	arg1	cells					966:970	wild-type HEK293T cells	948:970	wild-type HEK293T cells	948:970	When HIV virions were generated in cells overexpressing polypeptide N-acetylgalactosaminyltransferase 1 (GalNAcT1), viral infectivity was increased 2.5-fold compared to that of virus produced in wild-type HEK293T cells; infectivity was increased 8-fold when the Thr499Ser mutant was used.
28747495	8	112	from	residue	1653:1659	arg1	question					1664:1671	question	1664:1671	question	1664:1671	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	0	113	theme	Immunodeficiency	6:21	arg1	Virus					23:27	Human Immunodeficiency Virus	0:27	Human Immunodeficiency Virus	0:27	Human Immunodeficiency Virus and Simian Immunodeficiency Virus Maintain High Levels of Infectivity in the Complete Absence of Mucin-Type O-Glycosylation.
28747495	2	114	dep	other	451:455	arg1	than					457:460	than	457:460	than	457:460	When this highly conserved Thr residue was substituted with anything other than serine (the other amino acid that can accept O-glycosylation), the resulting virus was noninfectious.
28747495	4	115	theme	Thr499Ser	1015:1023	arg1	mutant					1025:1030	the Thr499Ser mutant	1011:1030	the Thr499Ser mutant	1011:1030	When HIV virions were generated in cells overexpressing polypeptide N-acetylgalactosaminyltransferase 1 (GalNAcT1), viral infectivity was increased 2.5-fold compared to that of virus produced in wild-type HEK293T cells; infectivity was increased 8-fold when the Thr499Ser mutant was used.
28747495	8	116	theme	target	1567:1572	arg1	cells					1574:1578	natural target cells	1559:1578	natural target cells for HIV and SIV	1559:1594	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	14	117	theme	O-linked	2412:2419	arg1	glycosylation					2421:2433	O-linked glycosylation	2412:2433	O-linked glycosylation	2412:2433	Our studies described in this report demonstrate unambiguously that O-linked glycosylation is not necessary for the natural replication cycle of HIV and SIV.
28747495	3	118	theme	gp120	623:627	arg1	association					608:618	the association	604:618	the association of gp120 with the virion	604:643	We found that this Thr was critical for the association of gp120 with the virion and that amino acid substitution increased the amount of dissociated gp120 in the cell culture supernatant.
28747495	0	119	theme	Immunodeficiency	40:55	arg1	Virus					57:61	Simian Immunodeficiency Virus	33:61	Simian Immunodeficiency Virus	33:61	Human Immunodeficiency Virus and Simian Immunodeficiency Virus Maintain High Levels of Infectivity in the Complete Absence of Mucin-Type O-Glycosylation.
28747495	9	120	theme	mass	1824:1827	arg1	IMPORTANCE					1788:1797	IMPORTANCE	1788:1797	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV	1788:1882	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV is N-linked carbohydrate.
28747495	14	121	theme	natural	2460:2466	arg1	cycle					2480:2484	the natural replication cycle	2456:2484	the natural replication cycle of HIV and SIV	2456:2499	Our studies described in this report demonstrate unambiguously that O-linked glycosylation is not necessary for the natural replication cycle of HIV and SIV.
28747495	2	122	theme	conserved	399:407	arg1	residue					413:419	this highly conserved Thr residue	387:419	this highly conserved Thr residue	387:419	When this highly conserved Thr residue was substituted with anything other than serine (the other amino acid that can accept O-glycosylation), the resulting virus was noninfectious.
28747495	6	123	theme	cell	1138:1141	arg1	lines					1143:1147	HEK293T knockout cell lines	1121:1147	HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation	1121:1211	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	15	124	theme	transferase	2586:2596	arg1	enzymes					2598:2604	numerous GalNAc transferase enzymes	2570:2604	numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment	2570:2651	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	8	125	gly	O-glycosylation	1620:1634	arg1	residue					1653:1659	the threonine residue	1639:1659	the threonine residue in question	1639:1671	Nonetheless, it remains theoretically possible that the repertoire of GalNAc transferase isoforms in natural target cells for HIV and SIV in vivo could result in O-glycosylation of the threonine residue in question and that this could boost the infectivity of virions beyond the levels seen in the absence of such O-glycosylation.
28747495	6	126	theme	HEK293T	1121:1127	arg1	lines					1143:1147	HEK293T knockout cell lines	1121:1147	HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation	1121:1211	Using HEK293T knockout cell lines totally devoid of the ability to perform O-linked glycosylation, we demonstrated production of normal levels of virions and normal levels of infectivity in the complete absence of O-linked carbohydrate.
28747495	9	127	theme	envelope	1842:1849	arg1	glycoprotein					1851:1862	the gp120 envelope glycoprotein	1832:1862	the gp120 envelope glycoprotein of both HIV and SIV	1832:1882	IMPORTANCE Approximately 50% of the mass of the gp120 envelope glycoprotein of both HIV and SIV is N-linked carbohydrate.
28747495	4	128	theme	HIV	758:760	arg1	virions					762:768	HIV virions	758:768	HIV virions	758:768	When HIV virions were generated in cells overexpressing polypeptide N-acetylgalactosaminyltransferase 1 (GalNAcT1), viral infectivity was increased 2.5-fold compared to that of virus produced in wild-type HEK293T cells; infectivity was increased 8-fold when the Thr499Ser mutant was used.
28747495	15	129	theme	numerous	2570:2577	arg1	enzymes					2598:2604	numerous GalNAc transferase enzymes	2570:2604	numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment	2570:2651	However, the door is not totally closed because of the diversity of numerous GalNAc transferase enzymes that initiate O-linked carbohydrate attachment and the theoretical possibility that natural target cells for HIV and SIV in vivo could potentially complete such O-linked carbohydrate attachment to further increase infectivity.
28747495	1	130	theme	virus	238:242	arg1	gp120					206:210	gp120	206:210	gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV)	206:288	A highly conserved threonine near the C terminus of gp120 of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) was investigated for its contributions to envelope protein function and virion infectivity.
28747495	11	131	link	N-linked	2049:2056	arg1	glycosylation					2058:2070	This N-linked glycosylation	2044:2070	This N-linked glycosylation	2044:2070	This N-linked glycosylation is one of the reasons that primary isolates of HIV and SIV are so heavily resistant to antibody-mediated neutralization.
28032681	7	0	theme	in	1228:1229	arg1	application					1236:1246	in vivo application	1228:1246	in vivo application	1228:1246	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	0	1	from	study	8:12	arg1	biocompatibility					21:36	the biocompatibility	17:36	the biocompatibility of chitosan-hydroxyapatite film	17:68	In vivo study on the biocompatibility of chitosan-hydroxyapatite film depending on degree of deacetylation.
28032681	5	2	theme	nuclear	910:916	arg1	resonance					927:935	proton nuclear magnetic resonance	903:935	proton nuclear magnetic resonance spectroscopy	903:948	The DOD of the chitosan obtained from three different manufacturers was estimated and calculated by Raman spectroscopy, Fourier transform infrared spectroscopy, and proton nuclear magnetic resonance spectroscopy.
28032681	7	3	with	products	1370:1377	arg1	DOD					1393:1395	the same DOD	1384:1395	the same DOD	1384:1395	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	8	4	theme	chitosan-based	1511:1524	arg1	researches					1526:1535	further chitosan-based researches	1503:1535	further chitosan-based researches	1503:1535	This basic study on the relationship between the DOD and inflammation is valuable for the development of further chitosan-based researches.
28032681	5	5	theme	magnetic	918:925	arg1	resonance					927:935	proton nuclear magnetic resonance	903:935	proton nuclear magnetic resonance spectroscopy	903:948	The DOD of the chitosan obtained from three different manufacturers was estimated and calculated by Raman spectroscopy, Fourier transform infrared spectroscopy, and proton nuclear magnetic resonance spectroscopy.
28032681	6	6	theme	inflammation	1014:1025	arg1	incidence					1001:1009	a higher incidence	992:1009	a higher incidence of inflammation in skin cells	992:1039	The chitosan with the higher DOD induced a higher incidence of inflammation in skin cells.
28032681	0	7	theme	deacetylation	93:105	arg1	degree					83:88	degree	83:88	degree of deacetylation	83:105	In vivo study on the biocompatibility of chitosan-hydroxyapatite film depending on degree of deacetylation.
28032681	5	8	dep	Fourier	858:864	arg1	transform					866:874	transform	866:874	transform infrared spectroscopy	866:896	The DOD of the chitosan obtained from three different manufacturers was estimated and calculated by Raman spectroscopy, Fourier transform infrared spectroscopy, and proton nuclear magnetic resonance spectroscopy.
28032681	5	9	theme	proton	903:908	arg1	resonance					927:935	proton nuclear magnetic resonance	903:935	proton nuclear magnetic resonance spectroscopy	903:948	The DOD of the chitosan obtained from three different manufacturers was estimated and calculated by Raman spectroscopy, Fourier transform infrared spectroscopy, and proton nuclear magnetic resonance spectroscopy.
28032681	4	10	theme	chitosan-hydroxyapatite	556:578	arg1	films					590:594	three chitosan-hydroxyapatite composite films	550:594	three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values	550:641	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	7	11	dep	group	1052:1056	arg1	density					1058:1064	density	1058:1064	density	1058:1064	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	7	11	dep	group	1052:1056	arg1	biodegradability					1067:1082	biodegradability	1067:1082	biodegradability	1067:1082	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	7	11	dep	group	1052:1056	arg1	crystallinity					1089:1101	crystallinity	1089:1101	crystallinity	1089:1101	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	7	12	theme	possible	1129:1136	arg1	factors					1138:1144	the three possible factors	1119:1144	the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD	1119:1395	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	7	12	theme	possible	1129:1136	arg1	group					1052:1056	The amino group density, biodegradability, and crystallinity	1042:1101	The amino group density, biodegradability, and crystallinity of chitosan	1042:1113	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	7	12	theme	possible	1129:1136	arg1	chitosan					1106:1113	chitosan	1106:1113	chitosan	1106:1113	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	8	13	from	study	1409:1413	arg1	relationship					1422:1433	the relationship	1418:1433	the relationship between the DOD and inflammation	1418:1466	This basic study on the relationship between the DOD and inflammation is valuable for the development of further chitosan-based researches.
28032681	7	14	theme	biological	1276:1285	arg1	results					1287:1293	complicated biological results	1264:1293	complicated biological results	1264:1293	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	4	15	dep	in	522:523	arg1	vivo					525:528	vivo	525:528	vivo	525:528	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	7	16	theme	better	1316:1321	arg1	inflammation					1332:1343	either better or worse inflammation	1309:1343	either better or worse inflammation even when using chitosan products with the same DOD	1309:1395	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	6	17	from	incidence	1001:1009	arg1	cells					1035:1039	skin cells	1030:1039	skin cells	1030:1039	The chitosan with the higher DOD induced a higher incidence of inflammation in skin cells.
28032681	1	18	theme	polymers	154:161	arg1	one					143:145	one	143:145	one	143:145	Chitosan, produced from chitin, is one of the polymers with promising applications in various fields.
28032681	1	18	theme	polymers	154:161	arg1	polymers					154:161	the polymers	150:161	the polymers with promising applications in various fields	150:207	Chitosan, produced from chitin, is one of the polymers with promising applications in various fields.
28032681	4	19	theme	in	522:523	arg1	biocompatibility					530:545	the in vivo biocompatibility	518:545	the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values	518:641	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	10	20	dep	105A	1596:1599	arg1	1637-1645					1602:1610	1637-1645	1602:1610	1637-1645	1602:1610	J Biomed Mater Res Part A: 105A: 1637-1645, 2017.
28032681	9	21	dep	©	1538:1538	arg1	Inc.					1564:1567	Inc.	1564:1567	Inc.	1564:1567	© 2017 Wiley Periodicals, Inc.
28032681	8	22	theme	researches	1526:1535	arg1	development					1488:1498	the development	1484:1498	the development of further chitosan-based researches	1484:1535	This basic study on the relationship between the DOD and inflammation is valuable for the development of further chitosan-based researches.
28032681	4	23	theme	DOD	632:634	arg1	values					636:641	different DOD values	622:641	different DOD values	622:641	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	0	24	theme	In	0:1	arg1	study					8:12	In vivo study	0:12	In vivo study on the biocompatibility of chitosan-hydroxyapatite film	0:68	In vivo study on the biocompatibility of chitosan-hydroxyapatite film depending on degree of deacetylation.
28032681	6	25	theme	higher	994:999	arg1	incidence					1001:1009	a higher incidence	992:1009	a higher incidence of inflammation in skin cells	992:1039	The chitosan with the higher DOD induced a higher incidence of inflammation in skin cells.
28032681	7	26	theme	chitosan	1106:1113	arg1	factors					1138:1144	the three possible factors	1119:1144	the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD	1119:1395	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	7	26	theme	chitosan	1106:1113	arg1	group					1052:1056	The amino group density, biodegradability, and crystallinity	1042:1101	The amino group density, biodegradability, and crystallinity of chitosan	1042:1113	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	7	26	theme	chitosan	1106:1113	arg1	chitosan					1106:1113	chitosan	1106:1113	chitosan	1106:1113	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	6	27	with	chitosan	955:962	arg1	DOD					980:982	the higher DOD	969:982	the higher DOD	969:982	The chitosan with the higher DOD induced a higher incidence of inflammation in skin cells.
28032681	1	28	theme	promising	168:176	arg1	applications					178:189	promising applications	168:189	promising applications in various fields	168:207	Chitosan, produced from chitin, is one of the polymers with promising applications in various fields.
28032681	7	29	dep	in	1228:1229	arg1	vivo					1231:1234	vivo	1231:1234	vivo	1231:1234	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	5	30	theme	Raman	838:842	arg1	spectroscopy					844:855	Raman spectroscopy	838:855	Raman spectroscopy	838:855	The DOD of the chitosan obtained from three different manufacturers was estimated and calculated by Raman spectroscopy, Fourier transform infrared spectroscopy, and proton nuclear magnetic resonance spectroscopy.
28032681	3	31	theme	deacetylation	348:360	arg1	reason					324:329	The main reason	315:329	The main reason	315:329	The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property.
28032681	3	31	theme	deacetylation	348:360	arg1	degree					338:343	the degree	334:343	the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property	334:498	The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property.
28032681	4	32	theme	biological	675:684	arg1	protocols					686:694	traditional biological protocols	663:694	traditional biological protocols	663:694	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	4	33	theme	novel	700:704	arg1	analyses					728:735	novel optical spectroscopic analyses	700:735	novel optical spectroscopic analyses	700:735	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	10	34	dep	Res	1584:1586	arg1	105A					1596:1599	105A	1596:1599	105A	1596:1599	J Biomed Mater Res Part A: 105A: 1637-1645, 2017.
28032681	10	34	dep	Res	1584:1586	arg1	A					1593:1593	Part A	1588:1593	J Biomed Mater Res Part A: 105A: 1637-1645, 2017.	1569:1617	J Biomed Mater Res Part A: 105A: 1637-1645, 2017.
28032681	1	35	theme	various	194:200	arg1	fields					202:207	various fields	194:207	various fields	194:207	Chitosan, produced from chitin, is one of the polymers with promising applications in various fields.
28032681	7	36	theme	same	1388:1391	arg1	DOD					1393:1395	the same DOD	1384:1395	the same DOD	1384:1395	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	7	37	theme	worse	1326:1330	arg1	inflammation					1332:1343	either better or worse inflammation	1309:1343	either better or worse inflammation even when using chitosan products with the same DOD	1309:1395	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	3	38	theme	units	417:421	arg1	proportion					390:399	the proportion	386:399	the proportion of deacetylated units in the polymer	386:436	The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property.
28032681	5	39	theme	different	782:790	arg1	manufacturers					792:804	three different manufacturers	776:804	three different manufacturers	776:804	The DOD of the chitosan obtained from three different manufacturers was estimated and calculated by Raman spectroscopy, Fourier transform infrared spectroscopy, and proton nuclear magnetic resonance spectroscopy.
28032681	6	40	theme	higher	973:978	arg1	DOD					980:982	the higher DOD	969:982	the higher DOD	969:982	The chitosan with the higher DOD induced a higher incidence of inflammation in skin cells.
28032681	3	41	from	units	417:421	arg1	polymer					430:436	the polymer	426:436	the polymer	426:436	The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property.
28032681	4	42	theme	different	622:630	arg1	values					636:641	different DOD values	622:641	different DOD values	622:641	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	0	43	theme	chitosan-hydroxyapatite	41:63	arg1	film					65:68	chitosan-hydroxyapatite film	41:68	chitosan-hydroxyapatite film	41:68	In vivo study on the biocompatibility of chitosan-hydroxyapatite film depending on degree of deacetylation.
28032681	7	44	theme	films	1218:1222	arg1	biocompatibility					1194:1209	the biocompatibility	1190:1209	the biocompatibility of the films for in vivo application	1190:1246	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	0	45	theme	film	65:68	arg1	biocompatibility					21:36	the biocompatibility	17:36	the biocompatibility of chitosan-hydroxyapatite film	17:68	In vivo study on the biocompatibility of chitosan-hydroxyapatite film depending on degree of deacetylation.
28032681	5	46	dep	transform	866:874	arg1	infrared					876:883	infrared	876:883	transform infrared spectroscopy	866:896	The DOD of the chitosan obtained from three different manufacturers was estimated and calculated by Raman spectroscopy, Fourier transform infrared spectroscopy, and proton nuclear magnetic resonance spectroscopy.
28032681	4	47	theme	optical	706:712	arg1	analyses					728:735	novel optical spectroscopic analyses	700:735	novel optical spectroscopic analyses	700:735	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	8	48	theme	further	1503:1509	arg1	researches					1526:1535	further chitosan-based researches	1503:1535	further chitosan-based researches	1503:1535	This basic study on the relationship between the DOD and inflammation is valuable for the development of further chitosan-based researches.
28032681	8	49	dep	DOD	1447:1449	arg1	the					1443:1445	the	1443:1445	the	1443:1445	This basic study on the relationship between the DOD and inflammation is valuable for the development of further chitosan-based researches.
28032681	8	50	theme	basic	1403:1407	arg1	study					1409:1413	This basic study	1398:1413	This basic study on the relationship between the DOD and inflammation	1398:1466	This basic study on the relationship between the DOD and inflammation is valuable for the development of further chitosan-based researches.
28032681	1	51	from	applications	178:189	arg1	fields					202:207	various fields	194:207	various fields	194:207	Chitosan, produced from chitin, is one of the polymers with promising applications in various fields.
28032681	2	52	dep	its	287:289	arg1	uses					291:294	uses	291:294	uses	291:294	However, despite diverse research studies conducted on its biocompatibility, its uses are still limited.
28032681	0	53	dep	In	0:1	arg1	vivo					3:6	vivo	3:6	vivo	3:6	In vivo study on the biocompatibility of chitosan-hydroxyapatite film depending on degree of deacetylation.
28032681	7	54	theme	amino	1046:1050	arg1	factors					1138:1144	the three possible factors	1119:1144	the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD	1119:1395	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	7	54	theme	amino	1046:1050	arg1	group					1052:1056	The amino group density, biodegradability, and crystallinity	1042:1101	The amino group density, biodegradability, and crystallinity of chitosan	1042:1113	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	7	54	theme	amino	1046:1050	arg1	chitosan					1106:1113	chitosan	1106:1113	chitosan	1106:1113	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	7	55	theme	chitosan	1361:1368	arg1	products					1370:1377	chitosan products	1361:1377	chitosan products with the same DOD	1361:1395	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	3	56	from	polymer	430:436	arg1	proportion					390:399	the proportion	386:399	the proportion of deacetylated units in the polymer	386:436	The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property.
28032681	7	57	theme	complicated	1264:1274	arg1	results					1287:1293	complicated biological results	1264:1293	complicated biological results	1264:1293	The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD.
28032681	4	58	theme	films	590:594	arg1	biocompatibility					530:545	the in vivo biocompatibility	518:545	the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values	518:641	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	1	59	with	polymers	154:161	arg1	applications					178:189	promising applications	168:189	promising applications in various fields	168:207	Chitosan, produced from chitin, is one of the polymers with promising applications in various fields.
28032681	5	60	theme	resonance	927:935	arg1	spectroscopy					937:948	proton nuclear magnetic resonance spectroscopy	903:948	proton nuclear magnetic resonance spectroscopy	903:948	The DOD of the chitosan obtained from three different manufacturers was estimated and calculated by Raman spectroscopy, Fourier transform infrared spectroscopy, and proton nuclear magnetic resonance spectroscopy.
28032681	5	61	theme	chitosan	753:760	arg1	DOD					742:744	The DOD	738:744	The DOD of the chitosan obtained from three different manufacturers	738:804	The DOD of the chitosan obtained from three different manufacturers was estimated and calculated by Raman spectroscopy, Fourier transform infrared spectroscopy, and proton nuclear magnetic resonance spectroscopy.
28032681	4	62	theme	spectroscopic	714:726	arg1	analyses					728:735	novel optical spectroscopic analyses	700:735	novel optical spectroscopic analyses	700:735	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	4	63	theme	composite	580:588	arg1	films					590:594	three chitosan-hydroxyapatite composite films	550:594	three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values	550:641	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	4	64	with	chitosan	608:615	arg1	values					636:641	different DOD values	622:641	different DOD values	622:641	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	2	65	theme	research	235:242	arg1	studies					244:250	diverse research studies	227:250	diverse research studies conducted on its biocompatibility	227:284	However, despite diverse research studies conducted on its biocompatibility, its uses are still limited.
28032681	3	66	from	proportion	390:399	arg1	polymer					430:436	the polymer	426:436	the polymer	426:436	The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property.
28032681	3	67	theme	biocompatibility	474:489	arg1	property					491:498	its biocompatibility property	470:498	its biocompatibility property	470:498	The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property.
28032681	3	68	theme	deacetylated	404:415	arg1	units					417:421	deacetylated units	404:421	deacetylated units in the polymer	404:436	The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property.
28032681	3	69	theme	main	319:322	arg1	reason					324:329	The main reason	315:329	The main reason	315:329	The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property.
28032681	3	69	theme	main	319:322	arg1	degree					338:343	the degree	334:343	the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property	334:498	The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property.
28032681	2	70	theme	diverse	227:233	arg1	studies					244:250	diverse research studies	227:250	diverse research studies conducted on its biocompatibility	227:284	However, despite diverse research studies conducted on its biocompatibility, its uses are still limited.
28032681	10	71	theme	Part	1588:1591	arg1	A					1593:1593	Part A	1588:1593	J Biomed Mater Res Part A: 105A: 1637-1645, 2017.	1569:1617	J Biomed Mater Res Part A: 105A: 1637-1645, 2017.
28032681	4	72	theme	traditional	663:673	arg1	protocols					686:694	traditional biological protocols	663:694	traditional biological protocols	663:694	In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses.
28032681	6	73	theme	skin	1030:1033	arg1	cells					1035:1039	skin cells	1030:1039	skin cells	1030:1039	The chitosan with the higher DOD induced a higher incidence of inflammation in skin cells.
27508491	11	0	theme	antibacterial	1663:1675	arg1	studies					1677:1683	In vitro antibacterial studies	1654:1683	In vitro antibacterial studies	1654:1683	In vitro antibacterial studies revealed that the gel system inhibited bacterial growth to a great extent.
27508491	3	1	theme	tigecycline	578:588	arg1	nanoparticles					590:602	tigecycline nanoparticles	578:602	tigecycline nanoparticles	578:602	In the present work tigecycline nanoparticles were loaded into chitosan-platelet-rich plasma (PRP) hydrogel.
27508491	12	2	theme	ex	1814:1815	arg1	assay					1835:1839	ex vivo porcine skin assay	1814:1839	ex vivo porcine skin assay	1814:1839	The antibacterial activity was further analyzed using ex vivo porcine skin assay.
27508491	3	3	theme	present	565:571	arg1	work					573:576	the present work	561:576	the present work	561:576	In the present work tigecycline nanoparticles were loaded into chitosan-platelet-rich plasma (PRP) hydrogel.
27508491	9	4	theme	viability	1453:1461	arg1	results					1463:1469	Cell viability results	1448:1469	Cell viability results	1448:1469	Cell viability results showed that these nanoparticles were cyto-compatible.
27508491	12	5	theme	antibacterial	1764:1776	arg1	activity					1778:1785	The antibacterial activity	1760:1785	The antibacterial activity	1760:1785	The antibacterial activity was further analyzed using ex vivo porcine skin assay.
27508491	6	6	theme	systems	1188:1194	arg1	property					1121:1128	the shear thinning property	1102:1128	the shear thinning property	1102:1128	Rheology studies have confirmed the shear thinning property, thermal stability, and injectability of the prepared gel systems.
27508491	6	6	theme	systems	1188:1194	arg1	stability					1139:1147	thermal stability	1131:1147	thermal stability	1131:1147	Rheology studies have confirmed the shear thinning property, thermal stability, and injectability of the prepared gel systems.
27508491	6	6	theme	systems	1188:1194	arg1	injectability					1154:1166	injectability	1154:1166	injectability	1154:1166	Rheology studies have confirmed the shear thinning property, thermal stability, and injectability of the prepared gel systems.
27508491	1	7	theme	injectable	211:220	arg1	system					231:236	an injectable hydrogel system	208:236	an injectable hydrogel system	208:236	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	6	8	theme	prepared	1175:1182	arg1	systems					1188:1194	the prepared gel systems	1171:1194	the prepared gel systems	1171:1194	Rheology studies have confirmed the shear thinning property, thermal stability, and injectability of the prepared gel systems.
27508491	10	9	theme	cell	1614:1617	arg1	proliferation					1619:1631	cell proliferation	1614:1631	cell proliferation	1614:1631	The bioactivity of PRP loaded chitosan gel toward fibroblast cell line was studied using cell proliferation and migration assay.
27508491	1	10	theme	system	231:236	arg1	use					201:203	the use	197:203	the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections	197:374	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	4	11	dep	transform	913:921	arg1	infrared					923:930	infrared	923:930	transform infrared spectroscopy (FT-IR)	913:951	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	3	12	theme	plasma	644:649	arg1	hydrogel					657:664	chitosan-platelet-rich plasma (PRP) hydrogel	621:664	chitosan-platelet-rich plasma (PRP) hydrogel	621:664	In the present work tigecycline nanoparticles were loaded into chitosan-platelet-rich plasma (PRP) hydrogel.
27508491	1	13	theme	current	167:173	arg1	modalities					185:194	the current treatment modalities	163:194	the current treatment modalities	163:194	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	8	14	theme	blood-clotting	1331:1344	arg1	assays					1346:1351	blood-clotting assays	1331:1351	blood-clotting assays	1331:1351	Hemolysis and blood-clotting assays demonstrated that the gel system was neither a hemolysin nor a hamper to the clotting cascade.
27508491	1	15	theme	wound	359:363	arg1	infections					365:374	Staphylococcus aureus (S. aureus) chronic wound infections	317:374	Staphylococcus aureus (S. aureus) chronic wound infections	317:374	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	2	16	theme	growth	527:532	arg1	inhibition					503:512	inhibition	503:512	inhibition of bacterial growth	503:532	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	2	16	theme	growth	527:532	arg1	healing					549:555	increased healing	539:555	increased healing	539:555	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	2	16	theme	growth	527:532	arg1	adhesiveness					415:426	adhesiveness	415:426	adhesiveness to infection site	415:444	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	2	16	theme	growth	527:532	arg1	frequency					455:463	reduced frequency	447:463	reduced frequency of dressings	447:476	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	2	16	theme	growth	527:532	arg1	release					494:500	sustained drug release	479:500	sustained drug release	479:500	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	4	17	theme	ionic	735:739	arg1	method					755:760	ionic cross-linking method	735:760	ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline	735:810	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	12	18	dep	ex	1814:1815	arg1	vivo					1817:1820	vivo	1817:1820	vivo	1817:1820	The antibacterial activity was further analyzed using ex vivo porcine skin assay.
27508491	0	19	theme	melanogaster	109:120	arg1	Model					122:126	Drosophila melanogaster Model	98:126	Drosophila melanogaster Model for Infectious Wounds	98:148	Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel Using Drosophila melanogaster Model for Infectious Wounds.
27508491	14	20	theme	antibacterial	2058:2070	arg1	activity					2072:2079	a significant antibacterial activity	2044:2079	a significant antibacterial activity against S. aureus	2044:2097	The tigecycline and tigecycline nanoparticle incorporated chitosan gel showed a significant antibacterial activity against S. aureus.
27508491	4	21	dep	Fourier	905:911	arg1	transform					913:921	transform	913:921	transform infrared spectroscopy (FT-IR)	913:951	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	15	22	theme	effective	2127:2135	arg1	system					2114:2119	the gel system	2106:2119	the gel system	2106:2119	Thus, the gel system is an effective medium for antibiotic delivery and can be applied on the infection sites to effectively forestall various skin infections caused by S. aureus.
27508491	15	22	theme	effective	2127:2135	arg1	medium					2137:2142	an effective medium	2124:2142	an effective medium for antibiotic delivery	2124:2166	Thus, the gel system is an effective medium for antibiotic delivery and can be applied on the infection sites to effectively forestall various skin infections caused by S. aureus.
27508491	6	23	theme	shear	1106:1110	arg1	property					1121:1128	the shear thinning property	1102:1128	the shear thinning property	1102:1128	Rheology studies have confirmed the shear thinning property, thermal stability, and injectability of the prepared gel systems.
27508491	2	24	theme	drug	489:492	arg1	release					494:500	sustained drug release	479:500	sustained drug release	479:500	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	0	25	theme	Drosophila	98:107	arg1	Model					122:126	Drosophila melanogaster Model	98:126	Drosophila melanogaster Model for Infectious Wounds	98:148	Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel Using Drosophila melanogaster Model for Infectious Wounds.
27508491	0	26	theme	Anti-staphylococcal	0:18	arg1	Activity					20:27	Anti-staphylococcal Activity	0:27	Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel	0:90	Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel Using Drosophila melanogaster Model for Infectious Wounds.
27508491	1	27	theme	aureus	332:337	arg1	infections					365:374	Staphylococcus aureus (S. aureus) chronic wound infections	317:374	Staphylococcus aureus (S. aureus) chronic wound infections	317:374	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	5	28	theme	activated	988:996	arg1	powder					1002:1007	activated PRP powder	988:1007	activated PRP powder	988:1007	The synthesized nanoparticles and activated PRP powder were mixed with chitosan hydrogel to form a homogeneous gel.
27508491	6	29	theme	Rheology	1070:1077	arg1	studies					1079:1085	Rheology studies	1070:1085	Rheology studies	1070:1085	Rheology studies have confirmed the shear thinning property, thermal stability, and injectability of the prepared gel systems.
27508491	13	30	theme	hydrogels	1895:1903	arg1	activity					1870:1877	In vivo anti-Staphylococcal activity	1842:1877	In vivo anti-Staphylococcal activity of the prepared hydrogels	1842:1903	In vivo anti-Staphylococcal activity of the prepared hydrogels was studied using a Drosophila melanogaster infection model.
27508491	2	31	theme	reduced	447:453	arg1	frequency					455:463	reduced frequency	447:463	reduced frequency of dressings	447:476	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	0	32	theme	Nano	43:46	arg1	Hydrogel					83:90	Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel	32:90	Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel	32:90	Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel Using Drosophila melanogaster Model for Infectious Wounds.
27508491	10	33	theme	chitosan	1555:1562	arg1	gel					1564:1566	chitosan gel	1555:1566	chitosan gel	1555:1566	The bioactivity of PRP loaded chitosan gel toward fibroblast cell line was studied using cell proliferation and migration assay.
27508491	4	34	theme	scanning	865:872	arg1	SEM					895:897	SEM	895:897	SEM	895:897	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	4	34	theme	scanning	865:872	arg1	microscope					883:892	scanning electron microscope	865:892	scanning electron microscope (SEM)	865:898	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	0	35	theme	Composite	73:81	arg1	Hydrogel					83:90	Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel	32:90	Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel	32:90	Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel Using Drosophila melanogaster Model for Infectious Wounds.
27508491	10	36	theme	PRP	1544:1546	arg1	bioactivity					1529:1539	The bioactivity	1525:1539	The bioactivity of PRP loaded chitosan gel toward fibroblast cell line	1525:1594	The bioactivity of PRP loaded chitosan gel toward fibroblast cell line was studied using cell proliferation and migration assay.
27508491	8	37	theme	clotting	1430:1437	arg1	cascade					1439:1445	the clotting cascade	1426:1445	the clotting cascade	1426:1445	Hemolysis and blood-clotting assays demonstrated that the gel system was neither a hemolysin nor a hamper to the clotting cascade.
27508491	5	38	theme	chitosan	1025:1032	arg1	hydrogel					1034:1041	chitosan hydrogel	1025:1041	chitosan hydrogel	1025:1041	The synthesized nanoparticles and activated PRP powder were mixed with chitosan hydrogel to form a homogeneous gel.
27508491	12	39	theme	skin	1830:1833	arg1	assay					1835:1839	ex vivo porcine skin assay	1814:1839	ex vivo porcine skin assay	1814:1839	The antibacterial activity was further analyzed using ex vivo porcine skin assay.
27508491	10	40	theme	cell	1586:1589	arg1	line					1591:1594	fibroblast cell line	1575:1594	fibroblast cell line	1575:1594	The bioactivity of PRP loaded chitosan gel toward fibroblast cell line was studied using cell proliferation and migration assay.
27508491	15	41	theme	gel	2110:2112	arg1	system					2114:2119	the gel system	2106:2119	the gel system	2106:2119	Thus, the gel system is an effective medium for antibiotic delivery and can be applied on the infection sites to effectively forestall various skin infections caused by S. aureus.
27508491	15	41	theme	gel	2110:2112	arg1	medium					2137:2142	an effective medium	2124:2142	an effective medium for antibiotic delivery	2124:2166	Thus, the gel system is an effective medium for antibiotic delivery and can be applied on the infection sites to effectively forestall various skin infections caused by S. aureus.
27508491	1	42	theme	encapsulated	262:273	arg1	nanoparticles					275:287	antibiotic encapsulated nanoparticles	251:287	antibiotic encapsulated nanoparticles	251:287	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	6	43	theme	thermal	1131:1137	arg1	stability					1139:1147	thermal stability	1131:1147	thermal stability	1131:1147	Rheology studies have confirmed the shear thinning property, thermal stability, and injectability of the prepared gel systems.
27508491	13	44	theme	anti-Staphylococcal	1850:1868	arg1	activity					1870:1877	In vivo anti-Staphylococcal activity	1842:1877	In vivo anti-Staphylococcal activity of the prepared hydrogels	1842:1903	In vivo anti-Staphylococcal activity of the prepared hydrogels was studied using a Drosophila melanogaster infection model.
27508491	15	45	theme	skin	2243:2246	arg1	infections					2248:2257	various skin infections	2235:2257	various skin infections caused by S. aureus	2235:2277	Thus, the gel system is an effective medium for antibiotic delivery and can be applied on the infection sites to effectively forestall various skin infections caused by S. aureus.
27508491	13	46	theme	melanogaster	1936:1947	arg1	model					1959:1963	a Drosophila melanogaster infection model	1923:1963	a Drosophila melanogaster infection model	1923:1963	In vivo anti-Staphylococcal activity of the prepared hydrogels was studied using a Drosophila melanogaster infection model.
27508491	9	47	theme	Cell	1448:1451	arg1	results					1463:1469	Cell viability results	1448:1469	Cell viability results	1448:1469	Cell viability results showed that these nanoparticles were cyto-compatible.
27508491	4	48	theme	electron	874:881	arg1	SEM					895:897	SEM	895:897	SEM	895:897	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	4	48	theme	electron	874:881	arg1	microscope					883:892	scanning electron microscope	865:892	scanning electron microscope (SEM)	865:898	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	4	49	theme	±	701:701	arg1	nm					706:707	95 ± 13 nm	698:707	95 ± 13 nm	698:707	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	4	49	theme	±	701:701	arg1	nanoparticles					683:695	The tigecycline nanoparticles	667:695	The tigecycline nanoparticles (95 ± 13 nm)	667:708	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	10	50	theme	migration	1637:1645	arg1	assay					1647:1651	cell proliferation and migration assay	1614:1651	cell proliferation and migration assay	1614:1651	The bioactivity of PRP loaded chitosan gel toward fibroblast cell line was studied using cell proliferation and migration assay.
27508491	11	51	theme	gel	1703:1705	arg1	system					1707:1712	the gel system	1699:1712	the gel system	1699:1712	In vitro antibacterial studies revealed that the gel system inhibited bacterial growth to a great extent.
27508491	7	52	theme	drug	1241:1244	arg1	property					1254:1261	its drug release property	1237:1261	its drug release property	1237:1261	The gel system was further assessed for its drug release property and found that it was released in a sustained manner.
27508491	8	53	dep	neither	1390:1396	arg1	hemolysin					1400:1408	a hemolysin	1398:1408	a hemolysin	1398:1408	Hemolysis and blood-clotting assays demonstrated that the gel system was neither a hemolysin nor a hamper to the clotting cascade.
27508491	6	54	theme	gel	1184:1186	arg1	systems					1188:1194	the prepared gel systems	1171:1194	the prepared gel systems	1171:1194	Rheology studies have confirmed the shear thinning property, thermal stability, and injectability of the prepared gel systems.
27508491	10	55	theme	proliferation	1619:1631	arg1	assay					1647:1651	cell proliferation and migration assay	1614:1651	cell proliferation and migration assay	1614:1651	The bioactivity of PRP loaded chitosan gel toward fibroblast cell line was studied using cell proliferation and migration assay.
27508491	1	56	theme	hydrogel	222:229	arg1	system					231:236	an injectable hydrogel system	208:236	an injectable hydrogel system	208:236	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	11	57	dep	In	1654:1655	arg1	vitro					1657:1661	vitro	1657:1661	vitro	1657:1661	In vitro antibacterial studies revealed that the gel system inhibited bacterial growth to a great extent.
27508491	4	58	theme	tigecycline	671:681	arg1	nm					706:707	95 ± 13 nm	698:707	95 ± 13 nm	698:707	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	4	58	theme	tigecycline	671:681	arg1	nanoparticles					683:695	The tigecycline nanoparticles	667:695	The tigecycline nanoparticles (95 ± 13 nm)	667:708	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	15	59	theme	various	2235:2241	arg1	infections					2248:2257	various skin infections	2235:2257	various skin infections caused by S. aureus	2235:2277	Thus, the gel system is an effective medium for antibiotic delivery and can be applied on the infection sites to effectively forestall various skin infections caused by S. aureus.
27508491	15	60	theme	infection	2194:2202	arg1	sites					2204:2208	the infection sites	2190:2208	the infection sites	2190:2208	Thus, the gel system is an effective medium for antibiotic delivery and can be applied on the infection sites to effectively forestall various skin infections caused by S. aureus.
27508491	3	61	theme	chitosan-platelet-rich	621:642	arg1	PRP					652:654	PRP	652:654	PRP	652:654	In the present work tigecycline nanoparticles were loaded into chitosan-platelet-rich plasma (PRP) hydrogel.
27508491	3	61	theme	chitosan-platelet-rich	621:642	arg1	plasma					644:649	chitosan-platelet-rich plasma	621:649	chitosan-platelet-rich plasma (PRP) hydrogel	621:664	In the present work tigecycline nanoparticles were loaded into chitosan-platelet-rich plasma (PRP) hydrogel.
27508491	4	62	theme	cross-linking	741:753	arg1	method					755:760	ionic cross-linking method	735:760	ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline	735:810	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	4	63	theme	light	841:845	arg1	scattering					847:856	dynamic light scattering	833:856	dynamic light scattering (DLS)	833:862	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	4	63	theme	light	841:845	arg1	DLS					859:861	DLS	859:861	DLS	859:861	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	7	64	theme	release	1246:1252	arg1	property					1254:1261	its drug release property	1237:1261	its drug release property	1237:1261	The gel system was further assessed for its drug release property and found that it was released in a sustained manner.
27508491	1	65	theme	treatment	175:183	arg1	modalities					185:194	the current treatment modalities	163:194	the current treatment modalities	163:194	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	1	66	dep	aureus	332:337	arg1	aureus					343:348	S. aureus	340:348	S. aureus	340:348	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	13	67	theme	Drosophila	1925:1934	arg1	model					1959:1963	a Drosophila melanogaster infection model	1923:1963	a Drosophila melanogaster infection model	1923:1963	In vivo anti-Staphylococcal activity of the prepared hydrogels was studied using a Drosophila melanogaster infection model.
27508491	8	68	dep	a	1414:1414	arg1	hamper					1416:1421	hamper	1416:1421	hamper to the clotting cascade	1416:1445	Hemolysis and blood-clotting assays demonstrated that the gel system was neither a hemolysin nor a hamper to the clotting cascade.
27508491	2	69	theme	bacterial	517:525	arg1	growth					527:532	bacterial growth	517:532	bacterial growth	517:532	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	13	70	theme	infection	1949:1957	arg1	model					1959:1963	a Drosophila melanogaster infection model	1923:1963	a Drosophila melanogaster infection model	1923:1963	In vivo anti-Staphylococcal activity of the prepared hydrogels was studied using a Drosophila melanogaster infection model.
27508491	0	71	theme	Infectious	132:141	arg1	Wounds					143:148	Infectious Wounds	132:148	Infectious Wounds	132:148	Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel Using Drosophila melanogaster Model for Infectious Wounds.
27508491	1	72	theme	several	381:387	arg1	advantages					389:398	several advantages	381:398	several advantages	381:398	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	5	73	theme	PRP	998:1000	arg1	powder					1002:1007	activated PRP powder	988:1007	activated PRP powder	988:1007	The synthesized nanoparticles and activated PRP powder were mixed with chitosan hydrogel to form a homogeneous gel.
27508491	6	74	theme	thinning	1112:1119	arg1	property					1121:1128	the shear thinning property	1102:1128	the shear thinning property	1102:1128	Rheology studies have confirmed the shear thinning property, thermal stability, and injectability of the prepared gel systems.
27508491	2	75	theme	increased	539:547	arg1	healing					549:555	increased healing	539:555	increased healing	539:555	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	8	76	theme	gel	1375:1377	arg1	neither					1390:1396	neither	1390:1396	neither	1390:1396	Hemolysis and blood-clotting assays demonstrated that the gel system was neither a hemolysin nor a hamper to the clotting cascade.
27508491	8	76	theme	gel	1375:1377	arg1	system					1379:1384	the gel system	1371:1384	the gel system	1371:1384	Hemolysis and blood-clotting assays demonstrated that the gel system was neither a hemolysin nor a hamper to the clotting cascade.
27508491	2	77	theme	infection	431:439	arg1	site					441:444	infection site	431:444	infection site	431:444	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	7	78	theme	gel	1201:1203	arg1	system					1205:1210	The gel system	1197:1210	The gel system	1197:1210	The gel system was further assessed for its drug release property and found that it was released in a sustained manner.
27508491	13	79	theme	In	1842:1843	arg1	activity					1870:1877	In vivo anti-Staphylococcal activity	1842:1877	In vivo anti-Staphylococcal activity of the prepared hydrogels	1842:1903	In vivo anti-Staphylococcal activity of the prepared hydrogels was studied using a Drosophila melanogaster infection model.
27508491	5	80	theme	synthesized	958:968	arg1	nanoparticles					970:982	The synthesized nanoparticles	954:982	The synthesized nanoparticles	954:982	The synthesized nanoparticles and activated PRP powder were mixed with chitosan hydrogel to form a homogeneous gel.
27508491	2	81	theme	sustained	479:487	arg1	release					494:500	sustained drug release	479:500	sustained drug release	479:500	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	0	82	theme	Injectable	32:41	arg1	Hydrogel					83:90	Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel	32:90	Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel	32:90	Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel Using Drosophila melanogaster Model for Infectious Wounds.
27508491	2	83	theme	dressings	468:476	arg1	inhibition					503:512	inhibition	503:512	inhibition of bacterial growth	503:532	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	2	83	theme	dressings	468:476	arg1	healing					549:555	increased healing	539:555	increased healing	539:555	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	2	83	theme	dressings	468:476	arg1	adhesiveness					415:426	adhesiveness	415:426	adhesiveness to infection site	415:444	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	2	83	theme	dressings	468:476	arg1	frequency					455:463	reduced frequency	447:463	reduced frequency of dressings	447:476	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	2	83	theme	dressings	468:476	arg1	release					494:500	sustained drug release	479:500	sustained drug release	479:500	These include adhesiveness to infection site, reduced frequency of dressings, sustained drug release, inhibition of bacterial growth, and increased healing.
27508491	13	84	theme	prepared	1886:1893	arg1	hydrogels					1895:1903	the prepared hydrogels	1882:1903	the prepared hydrogels	1882:1903	In vivo anti-Staphylococcal activity of the prepared hydrogels was studied using a Drosophila melanogaster infection model.
27508491	0	85	theme	Tigecycline/Chitosan-PRP	48:71	arg1	Hydrogel					83:90	Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel	32:90	Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel	32:90	Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel Using Drosophila melanogaster Model for Infectious Wounds.
27508491	13	86	dep	In	1842:1843	arg1	vivo					1845:1848	vivo	1845:1848	vivo	1845:1848	In vivo anti-Staphylococcal activity of the prepared hydrogels was studied using a Drosophila melanogaster infection model.
27508491	14	87	theme	significant	2046:2056	arg1	activity					2072:2079	a significant antibacterial activity	2044:2079	a significant antibacterial activity against S. aureus	2044:2097	The tigecycline and tigecycline nanoparticle incorporated chitosan gel showed a significant antibacterial activity against S. aureus.
27508491	7	88	theme	sustained	1299:1307	arg1	manner					1309:1314	a sustained manner	1297:1314	a sustained manner	1297:1314	The gel system was further assessed for its drug release property and found that it was released in a sustained manner.
27508491	11	89	theme	bacterial	1724:1732	arg1	growth					1734:1739	bacterial growth	1724:1739	bacterial growth	1724:1739	In vitro antibacterial studies revealed that the gel system inhibited bacterial growth to a great extent.
27508491	14	90	dep	tigecycline	1970:1980	arg1	The					1966:1968	The	1966:1968	The	1966:1968	The tigecycline and tigecycline nanoparticle incorporated chitosan gel showed a significant antibacterial activity against S. aureus.
27508491	14	90	dep	tigecycline	1970:1980	arg1	nanoparticle					1998:2009	nanoparticle	1998:2009	nanoparticle	1998:2009	The tigecycline and tigecycline nanoparticle incorporated chitosan gel showed a significant antibacterial activity against S. aureus.
27508491	0	91	theme	Hydrogel	83:90	arg1	Activity					20:27	Anti-staphylococcal Activity	0:27	Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel	0:90	Anti-staphylococcal Activity of Injectable Nano Tigecycline/Chitosan-PRP Composite Hydrogel Using Drosophila melanogaster Model for Infectious Wounds.
27508491	5	92	theme	homogeneous	1053:1063	arg1	gel					1065:1067	a homogeneous gel	1051:1067	a homogeneous gel	1051:1067	The synthesized nanoparticles and activated PRP powder were mixed with chitosan hydrogel to form a homogeneous gel.
27508491	1	93	contain	have	376:379	arg1	use					201:203	the use	197:203	the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections	197:374	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	1	93	contain	have	376:379	arg2	advantages					389:398	several advantages	381:398	several advantages	381:398	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	1	94	theme	chronic	351:357	arg1	infections					365:374	Staphylococcus aureus (S. aureus) chronic wound infections	317:374	Staphylococcus aureus (S. aureus) chronic wound infections	317:374	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	14	95	theme	chitosan	2024:2031	arg1	gel					2033:2035	chitosan gel	2024:2035	chitosan gel	2024:2035	The tigecycline and tigecycline nanoparticle incorporated chitosan gel showed a significant antibacterial activity against S. aureus.
27508491	11	96	theme	great	1746:1750	arg1	extent					1752:1757	a great extent	1744:1757	a great extent	1744:1757	In vitro antibacterial studies revealed that the gel system inhibited bacterial growth to a great extent.
27508491	4	97	theme	dynamic	833:839	arg1	scattering					847:856	dynamic light scattering	833:856	dynamic light scattering (DLS)	833:862	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	4	97	theme	dynamic	833:839	arg1	DLS					859:861	DLS	859:861	DLS	859:861	The tigecycline nanoparticles (95 ± 13 nm) were synthesized through ionic cross-linking method using chitosan, tripolyphosphate, and tigecycline and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FT-IR).
27508491	11	98	theme	In	1654:1655	arg1	studies					1677:1683	In vitro antibacterial studies	1654:1683	In vitro antibacterial studies	1654:1683	In vitro antibacterial studies revealed that the gel system inhibited bacterial growth to a great extent.
27508491	1	99	theme	antibiotic	251:260	arg1	nanoparticles					275:287	antibiotic encapsulated nanoparticles	251:287	antibiotic encapsulated nanoparticles	251:287	Compared to the current treatment modalities, the use of an injectable hydrogel system, loaded with antibiotic encapsulated nanoparticles for the purpose of treating Staphylococcus aureus (S. aureus) chronic wound infections have several advantages.
27508491	15	100	theme	antibiotic	2148:2157	arg1	delivery					2159:2166	antibiotic delivery	2148:2166	antibiotic delivery	2148:2166	Thus, the gel system is an effective medium for antibiotic delivery and can be applied on the infection sites to effectively forestall various skin infections caused by S. aureus.
27508491	12	101	theme	porcine	1822:1828	arg1	assay					1835:1839	ex vivo porcine skin assay	1814:1839	ex vivo porcine skin assay	1814:1839	The antibacterial activity was further analyzed using ex vivo porcine skin assay.
27508491	10	102	theme	fibroblast	1575:1584	arg1	line					1591:1594	fibroblast cell line	1575:1594	fibroblast cell line	1575:1594	The bioactivity of PRP loaded chitosan gel toward fibroblast cell line was studied using cell proliferation and migration assay.
27497844	5	0	theme	wide	810:813	arg1	range					815:819	a wide range	808:819	a wide range of gelling ion concentrations	808:849	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	1	1	theme	material	260:267	arg1	class					251:255	an attractive class	237:255	an attractive class of material in the tissue engineering field	237:299	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	1	1	theme	material	260:267	arg1	hydrogels					223:231	hydrogels	223:231	hydrogels	223:231	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	3	2	theme	calcium	543:549	arg1	ions					551:554	calcium ions	543:554	calcium ions	543:554	In this study we have developed an experimental toolbox to measure the gelling kinetics of alginate upon crosslinking with calcium ions.
27497844	0	3	theme	Gelling	0:6	arg1	kinetics					8:15	Gelling kinetics	0:15	Gelling kinetics	0:15	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.
27497844	5	4	theme	concentrations	836:849	arg1	range					815:819	a wide range	808:819	a wide range of gelling ion concentrations	808:849	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	12	5	theme	hydrogel	2068:2075	arg1	systems					2077:2083	composite hydrogel systems	2058:2083	composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering	2058:2161	Our experimental system enables non-destructive study of composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering.
27497844	6	6	theme	developed	856:864	arg1	model					866:870	The developed model	852:870	The developed model	852:870	The developed model was used to predict the gelling time for a number of geometries, including microspheres typically used for cell encapsulation.
27497844	7	7	theme	correlative	1152:1162	arg1	microspectroscopy					1170:1186	correlative Raman microspectroscopy	1152:1186	correlative Raman microspectroscopy	1152:1186	We also demonstrate that this toolbox can be used to spatiotemporally investigate the formation and evolution of mineral within the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy.
27497844	7	8	used	used	1044:1047	arg2	toolbox					1029:1035	this toolbox	1024:1035	this toolbox	1024:1035	We also demonstrate that this toolbox can be used to spatiotemporally investigate the formation and evolution of mineral within the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy.
27497844	8	9	theme	Hydrogels	1275:1283	arg1	STATEMENT					1249:1257	STATEMENT	1249:1257	STATEMENT OF SIGNIFICANCE Hydrogels	1249:1283	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	10	10	theme	electron	1821:1828	arg1	microscopy					1830:1839	optical and electron microscopy	1809:1839	microscopy	1830:1839	This article describes the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme.
27497844	9	11	theme	phase	1487:1491	arg1	inclusion					1461:1469	The inclusion	1457:1469	The inclusion of an inorganic phase	1457:1491	The inclusion of an inorganic phase is one such approach and is known to affect both cell-material interactions and mechanical properties.
27497844	9	11	theme	phase	1487:1491	arg1	approach					1505:1512	one such approach	1496:1512	one such approach	1496:1512	The inclusion of an inorganic phase is one such approach and is known to affect both cell-material interactions and mechanical properties.
27497844	12	12	theme	non-destructive	2033:2047	arg1	study					2049:2053	non-destructive study	2033:2053	non-destructive study of composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering	2033:2161	Our experimental system enables non-destructive study of composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering.
27497844	4	13	theme	calcium	640:646	arg1	diffusion					627:635	the diffusion	623:635	the diffusion of calcium within the hydrogel	623:666	A reaction-diffusion model for gelation has been used to describe the diffusion of calcium within the hydrogel and was shown to match experimental observations well.
27497844	10	14	theme	experimental	1652:1663	arg1	approach					1665:1672	a correlative experimental approach	1638:1672	a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme	1638:1880	This article describes the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme.
27497844	1	15	from	material	260:267	arg1	field					295:299	the tissue engineering field	272:299	the tissue engineering field	272:299	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	5	16	theme	gelling	824:830	arg1	concentrations					836:849	gelling ion concentrations	824:849	gelling ion concentrations	824:849	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	1	17	from	class	251:255	arg1	field					295:299	the tissue engineering field	272:299	the tissue engineering field	272:299	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	10	18	theme	reaction-diffusion	1847:1864	arg1	scheme					1875:1880	a reaction-diffusion modeling scheme	1845:1880	a reaction-diffusion modeling scheme	1845:1880	This article describes the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme.
27497844	8	19	theme	tissue	1318:1323	arg1	engineering					1325:1335	cell-based tissue engineering	1307:1335	cell-based tissue engineering	1307:1335	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	12	20	theme	bone	2139:2142	arg1	engineering					2151:2161	bone tissue engineering	2139:2161	bone tissue engineering	2139:2161	Our experimental system enables non-destructive study of composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering.
27497844	7	21	theme	electron	1228:1235	arg1	microscopy					1237:1246	electron microscopy	1228:1246	electron microscopy	1228:1246	We also demonstrate that this toolbox can be used to spatiotemporally investigate the formation and evolution of mineral within the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy.
27497844	1	22	theme	water	152:156	arg1	content					158:164	their large water content	140:164	their large water content	140:164	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	6	23	theme	cell	979:982	arg1	encapsulation					984:996	cell encapsulation	979:996	cell encapsulation	979:996	The developed model was used to predict the gelling time for a number of geometries, including microspheres typically used for cell encapsulation.
27497844	8	24	theme	new	1346:1348	arg1	methods					1379:1385	new fabrication and modification methods	1346:1385	new fabrication and modification methods	1346:1385	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	5	25	theme	parameters	754:763	arg1	set					747:749	a single set	738:749	a single set of parameters	738:763	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	5	25	theme	parameters	754:763	arg1	able					769:772	able	769:772	able	769:772	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	5	25	theme	parameters	754:763	arg1	parameters					754:763	parameters	754:763	parameters	754:763	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	0	26	theme	spatiotemporal	81:94	arg1	toolbox					113:119	A correlative spatiotemporal characterization toolbox	67:119	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.	0:120	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.
27497844	10	27	theme	optical	1809:1815	arg1	microscopy					1830:1839	optical and electron microscopy	1809:1839	microscopy	1830:1839	This article describes the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme.
27497844	9	28	theme	mechanical	1573:1582	arg1	properties					1584:1593	mechanical properties	1573:1593	mechanical properties	1573:1593	The inclusion of an inorganic phase is one such approach and is known to affect both cell-material interactions and mechanical properties.
27497844	10	29	theme	gel	1680:1682	arg1	formation					1684:1692	gel formation	1680:1692	gel formation	1680:1692	This article describes the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme.
27497844	7	30	theme	Raman	1164:1168	arg1	microspectroscopy					1170:1186	correlative Raman microspectroscopy	1152:1186	correlative Raman microspectroscopy	1152:1186	We also demonstrate that this toolbox can be used to spatiotemporally investigate the formation and evolution of mineral within the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy.
27497844	1	31	theme	tissue	276:281	arg1	field					295:299	the tissue engineering field	272:299	the tissue engineering field	272:299	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	8	32	theme	full	1413:1416	arg1	potential					1418:1426	the full potential	1409:1426	the full potential of hydrogel based materials	1409:1454	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	2	33	theme	mild	403:406	arg1	conditions					408:417	mild conditions	403:417	mild conditions	403:417	Polymers capable of ionotropic gelation are of special interest due to their ability to form gels at mild conditions.
27497844	1	34	theme	structural	170:179	arg1	similarities					181:192	structural similarities	170:192	structural similarities to the extracellular matrix	170:220	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	7	35	theme	hydrogel	1131:1138	arg1	network					1140:1146	the hydrogel network	1127:1146	the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy	1127:1246	We also demonstrate that this toolbox can be used to spatiotemporally investigate the formation and evolution of mineral within the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy.
27497844	0	36	theme	hydrogels	56:64	arg1	mineralization					29:42	in situ mineralization	21:42	in situ mineralization	21:42	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.
27497844	0	36	theme	hydrogels	56:64	arg1	kinetics					8:15	Gelling kinetics	0:15	Gelling kinetics	0:15	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.
27497844	2	37	theme	special	349:355	arg1	interest					357:364	special interest	349:364	special interest	349:364	Polymers capable of ionotropic gelation are of special interest due to their ability to form gels at mild conditions.
27497844	8	38	theme	based	1440:1444	arg1	materials					1446:1454	hydrogel based materials	1431:1454	hydrogel based materials	1431:1454	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	10	39	theme	spatial	1740:1746	arg1	resolution					1761:1770	spatial and temporal resolution	1740:1770	spatial and temporal resolution	1740:1770	This article describes the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme.
27497844	11	40	theme	other	1934:1938	arg1	geometries					1940:1949	other geometries	1934:1949	other geometries	1934:1949	Modeling allows us to predict gelling kinetics for other geometries and sizes than those investigated experimentally.
27497844	10	41	theme	Raman	1784:1788	arg1	microspectroscopy					1790:1806	Raman microspectroscopy	1784:1806	Raman microspectroscopy	1784:1806	This article describes the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme.
27497844	1	42	theme	attractive	240:249	arg1	class					251:255	an attractive class	237:255	an attractive class of material in the tissue engineering field	237:299	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	1	42	theme	attractive	240:249	arg1	hydrogels					223:231	hydrogels	223:231	hydrogels	223:231	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	0	43	theme	alginate	47:54	arg1	hydrogels					56:64	alginate hydrogels	47:64	alginate hydrogels	47:64	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.
27497844	6	44	theme	gelling	896:902	arg1	time					904:907	the gelling time	892:907	the gelling time for a number of geometries, including microspheres typically used for cell encapsulation	892:996	The developed model was used to predict the gelling time for a number of geometries, including microspheres typically used for cell encapsulation.
27497844	7	45	theme	scanning	1204:1211	arg1	microscopy					1213:1222	confocal laser scanning microscopy	1189:1222	confocal laser scanning microscopy	1189:1222	We also demonstrate that this toolbox can be used to spatiotemporally investigate the formation and evolution of mineral within the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy.
27497844	3	46	theme	gelling	491:497	arg1	kinetics					499:506	the gelling kinetics	487:506	the gelling kinetics of alginate	487:518	In this study we have developed an experimental toolbox to measure the gelling kinetics of alginate upon crosslinking with calcium ions.
27497844	10	47	theme	temporal	1752:1759	arg1	resolution					1761:1770	spatial and temporal resolution	1740:1770	spatial and temporal resolution	1740:1770	This article describes the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme.
27497844	1	48	from	field	295:299	arg1	class					251:255	an attractive class	237:255	an attractive class of material in the tissue engineering field	237:299	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	1	48	from	field	295:299	arg1	hydrogels					223:231	hydrogels	223:231	hydrogels	223:231	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	5	49	theme	ion	832:834	arg1	concentrations					836:849	gelling ion concentrations	824:849	gelling ion concentrations	824:849	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	7	50	dep	formation	1085:1093	arg1	the					1081:1083	the	1081:1083	the	1081:1083	We also demonstrate that this toolbox can be used to spatiotemporally investigate the formation and evolution of mineral within the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy.
27497844	6	51	used	used	876:879	arg2	model					866:870	The developed model	852:870	The developed model	852:870	The developed model was used to predict the gelling time for a number of geometries, including microspheres typically used for cell encapsulation.
27497844	2	52	theme	ionotropic	322:331	arg1	gelation					333:340	ionotropic gelation	322:340	ionotropic gelation	322:340	Polymers capable of ionotropic gelation are of special interest due to their ability to form gels at mild conditions.
27497844	12	53	theme	composite	2058:2066	arg1	systems					2077:2083	composite hydrogel systems	2058:2083	composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering	2058:2161	Our experimental system enables non-destructive study of composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering.
27497844	3	54	with	crosslinking	525:536	arg1	ions					551:554	calcium ions	543:554	calcium ions	543:554	In this study we have developed an experimental toolbox to measure the gelling kinetics of alginate upon crosslinking with calcium ions.
27497844	2	55	theme	capable	311:317	arg1	Polymers					302:309	Polymers	302:309	Polymers capable of ionotropic gelation	302:340	Polymers capable of ionotropic gelation are of special interest due to their ability to form gels at mild conditions.
27497844	9	56	theme	inorganic	1477:1485	arg1	phase					1487:1491	an inorganic phase	1474:1491	an inorganic phase	1474:1491	The inclusion of an inorganic phase is one such approach and is known to affect both cell-material interactions and mechanical properties.
27497844	12	57	theme	systems	2077:2083	arg1	study					2049:2053	non-destructive study	2033:2053	non-destructive study of composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering	2033:2161	Our experimental system enables non-destructive study of composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering.
27497844	6	58	theme	geometries	925:934	arg1	microspheres					947:958	microspheres	947:958	microspheres typically used for cell encapsulation	947:996	The developed model was used to predict the gelling time for a number of geometries, including microspheres typically used for cell encapsulation.
27497844	6	58	theme	geometries	925:934	arg1	number					915:920	a number	913:920	a number of geometries, including microspheres typically used for cell encapsulation	913:996	The developed model was used to predict the gelling time for a number of geometries, including microspheres typically used for cell encapsulation.
27497844	8	59	theme	SIGNIFICANCE	1262:1273	arg1	Hydrogels					1275:1283	SIGNIFICANCE Hydrogels	1262:1283	SIGNIFICANCE Hydrogels	1262:1283	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	10	60	theme	approach	1665:1672	arg1	development					1623:1633	the development	1619:1633	the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme	1619:1880	This article describes the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme.
27497844	12	61	theme	relevant	2085:2092	arg1	systems					2077:2083	composite hydrogel systems	2058:2083	composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering	2058:2161	Our experimental system enables non-destructive study of composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering.
27497844	10	62	theme	correlative	1640:1650	arg1	approach					1665:1672	a correlative experimental approach	1638:1672	a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme	1638:1880	This article describes the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme.
27497844	7	63	theme	confocal	1189:1196	arg1	microscopy					1213:1222	confocal laser scanning microscopy	1189:1222	confocal laser scanning microscopy	1189:1222	We also demonstrate that this toolbox can be used to spatiotemporally investigate the formation and evolution of mineral within the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy.
27497844	5	64	from	particular	726:735	arg1	set					747:749	a single set	738:749	a single set of parameters	738:763	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	5	64	from	particular	726:735	arg1	able					769:772	able	769:772	able	769:772	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	5	64	from	particular	726:735	arg1	parameters					754:763	parameters	754:763	parameters	754:763	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	10	65	theme	modeling	1866:1873	arg1	scheme					1875:1880	a reaction-diffusion modeling scheme	1845:1880	a reaction-diffusion modeling scheme	1845:1880	This article describes the development of a correlative experimental approach where gel formation and mineralization has been investigated with spatial and temporal resolution by applying Raman microspectroscopy, optical and electron microscopy and a reaction-diffusion modeling scheme.
27497844	9	66	theme	such	1500:1503	arg1	approach					1505:1512	one such approach	1496:1512	one such approach	1496:1512	The inclusion of an inorganic phase is one such approach and is known to affect both cell-material interactions and mechanical properties.
27497844	9	66	theme	such	1500:1503	arg1	inclusion					1461:1469	The inclusion	1457:1469	The inclusion of an inorganic phase	1457:1491	The inclusion of an inorganic phase is one such approach and is known to affect both cell-material interactions and mechanical properties.
27497844	4	67	theme	experimental	691:702	arg1	observations					704:715	experimental observations	691:715	experimental observations	691:715	A reaction-diffusion model for gelation has been used to describe the diffusion of calcium within the hydrogel and was shown to match experimental observations well.
27497844	8	68	theme	cell-based	1307:1316	arg1	engineering					1325:1335	cell-based tissue engineering	1307:1335	cell-based tissue engineering	1307:1335	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	5	69	theme	single	740:745	arg1	set					747:749	a single set	738:749	a single set of parameters	738:763	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	5	69	theme	single	740:745	arg1	able					769:772	able	769:772	able	769:772	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	5	69	theme	single	740:745	arg1	parameters					754:763	parameters	754:763	parameters	754:763	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	1	70	theme	extracellular	201:213	arg1	matrix					215:220	the extracellular matrix	197:220	the extracellular matrix	197:220	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	1	71	theme	large	146:150	arg1	content					158:164	their large water content	140:164	their large water content	140:164	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	7	72	theme	laser	1198:1202	arg1	microscopy					1213:1222	confocal laser scanning microscopy	1189:1222	confocal laser scanning microscopy	1189:1222	We also demonstrate that this toolbox can be used to spatiotemporally investigate the formation and evolution of mineral within the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy.
27497844	0	73	theme	correlative	69:79	arg1	toolbox					113:119	A correlative spatiotemporal characterization toolbox	67:119	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.	0:120	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.
27497844	12	74	theme	tissue	2144:2149	arg1	engineering					2151:2161	bone tissue engineering	2139:2161	bone tissue engineering	2139:2161	Our experimental system enables non-destructive study of composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering.
27497844	0	75	theme	characterization	96:111	arg1	toolbox					113:119	A correlative spatiotemporal characterization toolbox	67:119	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.	0:120	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.
27497844	5	76	from	able	769:772	arg1	particular					726:735	particular	726:735	particular	726:735	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	9	77	theme	cell-material	1542:1554	arg1	interactions					1556:1567	cell-material interactions	1542:1567	cell-material interactions	1542:1567	The inclusion of an inorganic phase is one such approach and is known to affect both cell-material interactions and mechanical properties.
27497844	8	78	theme	fabrication	1350:1360	arg1	methods					1379:1385	new fabrication and modification methods	1346:1385	new fabrication and modification methods	1346:1385	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	11	79	theme	gelling	1913:1919	arg1	kinetics					1921:1928	gelling kinetics	1913:1928	gelling kinetics for other geometries and sizes than those investigated experimentally	1913:1998	Modeling allows us to predict gelling kinetics for other geometries and sizes than those investigated experimentally.
27497844	8	80	from	promise	1296:1302	arg1	engineering					1325:1335	cell-based tissue engineering	1307:1335	cell-based tissue engineering	1307:1335	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	8	81	theme	modification	1366:1377	arg1	methods					1379:1385	new fabrication and modification methods	1346:1385	new fabrication and modification methods	1346:1385	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	5	82	theme	gelation	785:792	arg1	kinetics					794:801	gelation kinetics	785:801	gelation kinetics	785:801	In particular, a single set of parameters was able to predict gelation kinetics over a wide range of gelling ion concentrations.
27497844	0	83	theme	in	21:22	arg1	mineralization					29:42	in situ mineralization	21:42	in situ mineralization	21:42	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.
27497844	1	84	theme	engineering	283:293	arg1	field					295:299	the tissue engineering field	272:299	the tissue engineering field	272:299	UNLABELLED Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field.
27497844	4	85	theme	reaction-diffusion	559:576	arg1	model					578:582	A reaction-diffusion model	557:582	A reaction-diffusion model for gelation	557:595	A reaction-diffusion model for gelation has been used to describe the diffusion of calcium within the hydrogel and was shown to match experimental observations well.
27497844	3	86	theme	experimental	455:466	arg1	toolbox					468:474	an experimental toolbox	452:474	an experimental toolbox	452:474	In this study we have developed an experimental toolbox to measure the gelling kinetics of alginate upon crosslinking with calcium ions.
27497844	8	87	theme	great	1290:1294	arg1	promise					1296:1302	great promise	1290:1302	great promise in cell-based tissue engineering	1290:1335	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	7	88	theme	mineral	1112:1118	arg1	evolution					1099:1107	evolution	1099:1107	evolution	1099:1107	We also demonstrate that this toolbox can be used to spatiotemporally investigate the formation and evolution of mineral within the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy.
27497844	7	88	theme	mineral	1112:1118	arg1	formation					1085:1093	formation	1085:1093	formation	1085:1093	We also demonstrate that this toolbox can be used to spatiotemporally investigate the formation and evolution of mineral within the hydrogel network via correlative Raman microspectroscopy, confocal laser scanning microscopy and electron microscopy.
27497844	12	89	theme	limited	2107:2113	arg1	applications					2119:2130	, but not limited to, applications	2097:2130	applications	2119:2130	Our experimental system enables non-destructive study of composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering.
27497844	4	90	used	used	606:609	arg2	model					578:582	A reaction-diffusion model	557:582	A reaction-diffusion model for gelation	557:595	A reaction-diffusion model for gelation has been used to describe the diffusion of calcium within the hydrogel and was shown to match experimental observations well.
27497844	0	91	dep	in	21:22	arg1	situ					24:27	situ	24:27	situ	24:27	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.
27497844	12	92	theme	experimental	2005:2016	arg1	system					2018:2023	Our experimental system	2001:2023	Our experimental system	2001:2023	Our experimental system enables non-destructive study of composite hydrogel systems relevant for, but not limited to, applications within bone tissue engineering.
27497844	8	93	theme	hydrogel	1431:1438	arg1	materials					1446:1454	hydrogel based materials	1431:1454	hydrogel based materials	1431:1454	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	2	94	theme	gelation	333:340	arg1	capable					311:317	capable	311:317	capable	311:317	Polymers capable of ionotropic gelation are of special interest due to their ability to form gels at mild conditions.
27497844	0	95	dep	kinetics	8:15	arg1	toolbox					113:119	A correlative spatiotemporal characterization toolbox	67:119	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.	0:120	Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.
27497844	8	96	theme	materials	1446:1454	arg1	potential					1418:1426	the full potential	1409:1426	the full potential of hydrogel based materials	1409:1454	STATEMENT OF SIGNIFICANCE Hydrogels show great promise in cell-based tissue engineering, however new fabrication and modification methods are needed to realize the full potential of hydrogel based materials.
27497844	3	97	theme	alginate	511:518	arg1	kinetics					499:506	the gelling kinetics	487:506	the gelling kinetics of alginate	487:518	In this study we have developed an experimental toolbox to measure the gelling kinetics of alginate upon crosslinking with calcium ions.
25996388	0	0	theme	Alginate	55:62	arg1	Hydrogel					64:71	an Efficient Sodium Alginate Hydrogel	35:71	an Efficient Sodium Alginate Hydrogel	35:71	Adsorption of Rare Earths(Ⅲ) Using an Efficient Sodium Alginate Hydrogel Cross-Linked with Poly-γ-Glutamate.
25996388	4	1	theme	immobilized	416:426	arg1	material					441:448	an immobilized gel particle material	413:448	an immobilized gel particle material	413:448	Through doping sodium alginate (SA) with poly-γ-glutamate (PGA), an immobilized gel particle material was produced.
25996388	1	2	theme	earth	139:143	arg1	ore					145:147	rare earth ore	134:147	rare earth ore	134:147	With the exploitation of rare earth ore, more and more REEs came into groundwater.
25996388	7	3	theme	REEs	816:819	arg1	pH					852:853	the pH	848:853	the pH of the solution	848:869	The factors that potentially affected the adsorption efficiency of the SA-PGA composite, including the initial concentration of REEs, the adsorbent dosage, and the pH of the solution, were investigated.
25996388	7	3	theme	REEs	816:819	arg1	concentration					799:811	the initial concentration	787:811	the initial concentration of REEs	787:819	The factors that potentially affected the adsorption efficiency of the SA-PGA composite, including the initial concentration of REEs, the adsorbent dosage, and the pH of the solution, were investigated.
25996388	7	3	theme	REEs	816:819	arg1	dosage					836:841	the adsorbent dosage	822:841	the adsorbent dosage	822:841	The factors that potentially affected the adsorption efficiency of the SA-PGA composite, including the initial concentration of REEs, the adsorbent dosage, and the pH of the solution, were investigated.
25996388	0	4	theme	Sodium	48:53	arg1	Hydrogel					64:71	an Efficient Sodium Alginate Hydrogel	35:71	an Efficient Sodium Alginate Hydrogel	35:71	Adsorption of Rare Earths(Ⅲ) Using an Efficient Sodium Alginate Hydrogel Cross-Linked with Poly-γ-Glutamate.
25996388	8	5	theme	single	911:916	arg1	solutions					936:944	single and mixed aqueous solutions	911:944	single and mixed aqueous solutions	911:944	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	7	6	theme	solution	862:869	arg1	pH					852:853	the pH	848:853	the pH of the solution	848:869	The factors that potentially affected the adsorption efficiency of the SA-PGA composite, including the initial concentration of REEs, the adsorbent dosage, and the pH of the solution, were investigated.
25996388	7	6	theme	solution	862:869	arg1	concentration					799:811	the initial concentration	787:811	the initial concentration of REEs	787:819	The factors that potentially affected the adsorption efficiency of the SA-PGA composite, including the initial concentration of REEs, the adsorbent dosage, and the pH of the solution, were investigated.
25996388	7	6	theme	solution	862:869	arg1	dosage					836:841	the adsorbent dosage	822:841	the adsorbent dosage	822:841	The factors that potentially affected the adsorption efficiency of the SA-PGA composite, including the initial concentration of REEs, the adsorbent dosage, and the pH of the solution, were investigated.
25996388	1	7	theme	ore	145:147	arg1	exploitation					118:129	the exploitation	114:129	the exploitation of rare earth ore	114:147	With the exploitation of rare earth ore, more and more REEs came into groundwater.
25996388	7	8	theme	initial	791:797	arg1	concentration					799:811	the initial concentration	787:811	the initial concentration of REEs	787:819	The factors that potentially affected the adsorption efficiency of the SA-PGA composite, including the initial concentration of REEs, the adsorbent dosage, and the pH of the solution, were investigated.
25996388	9	9	theme	SA-PGA	1267:1272	arg1	process					1237:1243	the adsorption process	1222:1243	the adsorption process	1222:1243	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	9	9	theme	SA-PGA	1267:1272	arg1	morphology					1253:1262	the morphology	1249:1262	the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA	1249:1352	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	3	10	theme	adsorption	299:308	arg1	material					310:317	an efficient adsorption material	286:317	an efficient adsorption material to mitigate the above issue	286:345	This study aimed to find an efficient adsorption material to mitigate the above issue.
25996388	4	11	theme	particle	432:439	arg1	material					441:448	an immobilized gel particle material	413:448	an immobilized gel particle material	413:448	Through doping sodium alginate (SA) with poly-γ-glutamate (PGA), an immobilized gel particle material was produced.
25996388	8	12	theme	aqueous	928:934	arg1	solutions					936:944	single and mixed aqueous solutions	911:944	single and mixed aqueous solutions	911:944	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	9	13	theme	compact	1291:1297	arg1	structure					1299:1307	the compact structure	1287:1307	the compact structure of SA to a porous structure after doping PGA	1287:1352	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	9	14	theme	adsorption	1226:1235	arg1	process					1237:1243	the adsorption process	1222:1243	the adsorption process	1222:1243	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	8	15	theme	mixed	922:926	arg1	solutions					936:944	single and mixed aqueous solutions	911:944	single and mixed aqueous solutions	911:944	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	10	16	theme	adsorption	1482:1491	arg1	model					1502:1506	the pseudo-second-order equation and the Langmuir adsorption isotherm model	1432:1506	the pseudo-second-order equation and the Langmuir adsorption isotherm model	1432:1506	The kinetics and thermodynamics of the adsorption of REEs were well fit with the pseudo-second-order equation and the Langmuir adsorption isotherm model, respectively.
25996388	10	17	theme	REEs	1408:1411	arg1	adsorption					1394:1403	the adsorption	1390:1403	the adsorption of REEs	1390:1411	The kinetics and thermodynamics of the adsorption of REEs were well fit with the pseudo-second-order equation and the Langmuir adsorption isotherm model, respectively.
25996388	10	18	theme	adsorption	1394:1403	arg1	thermodynamics					1372:1385	thermodynamics	1372:1385	thermodynamics	1372:1385	The kinetics and thermodynamics of the adsorption of REEs were well fit with the pseudo-second-order equation and the Langmuir adsorption isotherm model, respectively.
25996388	10	18	theme	adsorption	1394:1403	arg1	kinetics					1359:1366	kinetics	1359:1366	kinetics	1359:1366	The kinetics and thermodynamics of the adsorption of REEs were well fit with the pseudo-second-order equation and the Langmuir adsorption isotherm model, respectively.
25996388	9	19	theme	infrared	1075:1082	arg1	spectroscopy					1092:1103	infrared (FT-IR) spectroscopy	1075:1103	infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads	1075:1143	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	10	20	with	fit	1423:1425	arg1	model					1502:1506	the pseudo-second-order equation and the Langmuir adsorption isotherm model	1432:1506	the pseudo-second-order equation and the Langmuir adsorption isotherm model	1432:1506	The kinetics and thermodynamics of the adsorption of REEs were well fit with the pseudo-second-order equation and the Langmuir adsorption isotherm model, respectively.
25996388	3	21	theme	efficient	289:297	arg1	material					310:317	an efficient adsorption material	286:317	an efficient adsorption material to mitigate the above issue	286:345	This study aimed to find an efficient adsorption material to mitigate the above issue.
25996388	7	22	theme	composite	766:774	arg1	efficiency					741:750	the adsorption efficiency	726:750	the adsorption efficiency of the SA-PGA composite	726:774	The factors that potentially affected the adsorption efficiency of the SA-PGA composite, including the initial concentration of REEs, the adsorbent dosage, and the pH of the solution, were investigated.
25996388	1	23	theme	more	150:153	arg1	REEs					164:167	more and more REEs	150:167	more and more REEs	150:167	With the exploitation of rare earth ore, more and more REEs came into groundwater.
25996388	10	24	theme	pseudo-second-order	1436:1454	arg1	equation					1456:1463	pseudo-second-order equation	1436:1463	pseudo-second-order equation	1436:1463	The kinetics and thermodynamics of the adsorption of REEs were well fit with the pseudo-second-order equation and the Langmuir adsorption isotherm model, respectively.
25996388	10	25	dep	kinetics	1359:1366	arg1	The					1355:1357	The	1355:1357	The	1355:1357	The kinetics and thermodynamics of the adsorption of REEs were well fit with the pseudo-second-order equation and the Langmuir adsorption isotherm model, respectively.
25996388	9	26	theme	FT-IR	1085:1089	arg1	spectroscopy					1092:1103	infrared (FT-IR) spectroscopy	1075:1103	infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads	1075:1143	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	10	27	theme	isotherm	1493:1500	arg1	model					1502:1506	the pseudo-second-order equation and the Langmuir adsorption isotherm model	1432:1506	the pseudo-second-order equation and the Langmuir adsorption isotherm model	1432:1506	The kinetics and thermodynamics of the adsorption of REEs were well fit with the pseudo-second-order equation and the Langmuir adsorption isotherm model, respectively.
25996388	3	28	theme	above	335:339	arg1	issue					341:345	the above issue	331:345	the above issue	331:345	This study aimed to find an efficient adsorption material to mitigate the above issue.
25996388	10	29	theme	equation	1456:1463	arg1	model					1502:1506	the pseudo-second-order equation and the Langmuir adsorption isotherm model	1432:1506	the pseudo-second-order equation and the Langmuir adsorption isotherm model	1432:1506	The kinetics and thermodynamics of the adsorption of REEs were well fit with the pseudo-second-order equation and the Langmuir adsorption isotherm model, respectively.
25996388	5	30	theme	rare	521:524	arg1	REEs					542:545	REEs	542:545	REEs	542:545	The composite exhibited excellent capacity for adsorbing rare earth elements (REEs).
25996388	5	30	theme	rare	521:524	arg1	elements					532:539	rare earth elements	521:539	rare earth elements (REEs)	521:546	The composite exhibited excellent capacity for adsorbing rare earth elements (REEs).
25996388	1	31	theme	more	159:162	arg1	REEs					164:167	more and more REEs	150:167	more and more REEs	150:167	With the exploitation of rare earth ore, more and more REEs came into groundwater.
25996388	0	32	theme	Earths	19:24	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of Rare Earths(Ⅲ) Using an Efficient Sodium Alginate Hydrogel	0:71	Adsorption of Rare Earths(Ⅲ) Using an Efficient Sodium Alginate Hydrogel Cross-Linked with Poly-γ-Glutamate.
25996388	7	33	theme	adsorption	730:739	arg1	efficiency					741:750	the adsorption efficiency	726:750	the adsorption efficiency of the SA-PGA composite	726:774	The factors that potentially affected the adsorption efficiency of the SA-PGA composite, including the initial concentration of REEs, the adsorbent dosage, and the pH of the solution, were investigated.
25996388	5	34	theme	earth	526:530	arg1	REEs					542:545	REEs	542:545	REEs	542:545	The composite exhibited excellent capacity for adsorbing rare earth elements (REEs).
25996388	5	34	theme	earth	526:530	arg1	elements					532:539	rare earth elements	521:539	rare earth elements (REEs)	521:546	The composite exhibited excellent capacity for adsorbing rare earth elements (REEs).
25996388	7	35	theme	SA-PGA	759:764	arg1	composite					766:774	the SA-PGA composite	755:774	the SA-PGA composite	755:774	The factors that potentially affected the adsorption efficiency of the SA-PGA composite, including the initial concentration of REEs, the adsorbent dosage, and the pH of the solution, were investigated.
25996388	9	36	theme	SA	1312:1313	arg1	structure					1299:1307	the compact structure	1287:1307	the compact structure of SA to a porous structure after doping PGA	1287:1352	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	0	37	theme	Rare	14:17	arg1	Ⅲ					26:26	Ⅲ	26:26	Ⅲ	26:26	Adsorption of Rare Earths(Ⅲ) Using an Efficient Sodium Alginate Hydrogel Cross-Linked with Poly-γ-Glutamate.
25996388	0	37	theme	Rare	14:17	arg1	Earths					19:24	Rare Earths	14:24	Rare Earths(Ⅲ) Using an Efficient Sodium Alginate Hydrogel	14:71	Adsorption of Rare Earths(Ⅲ) Using an Efficient Sodium Alginate Hydrogel Cross-Linked with Poly-γ-Glutamate.
25996388	9	38	theme	Scanning	1018:1025	arg1	SEM					1048:1050	SEM	1048:1050	SEM	1048:1050	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	9	38	theme	Scanning	1018:1025	arg1	microscopy					1036:1045	Scanning electron microscopy	1018:1045	Scanning electron microscopy (SEM)	1018:1051	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	8	39	from	types	894:898	arg1	solutions					936:944	single and mixed aqueous solutions	911:944	single and mixed aqueous solutions	911:944	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	6	40	theme	gel	591:593	arg1	particles					595:603	the SA-PGA gel particles	580:603	the SA-PGA gel particles	580:603	The amount of La3+ adsorbed on the SA-PGA gel particles reached approximately 163.93 mg/g compared to the 81.97 mg/g adsorbed on SA alone.
25996388	10	41	theme	Langmuir	1473:1480	arg1	adsorption					1482:1491	the Langmuir adsorption	1469:1491	the Langmuir adsorption	1469:1491	The kinetics and thermodynamics of the adsorption of REEs were well fit with the pseudo-second-order equation and the Langmuir adsorption isotherm model, respectively.
25996388	9	42	theme	key	1210:1212	arg1	role					1214:1217	a key role	1208:1217	a key role	1208:1217	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	6	43	theme	SA-PGA	584:589	arg1	particles					595:603	the SA-PGA gel particles	580:603	the SA-PGA gel particles	580:603	The amount of La3+ adsorbed on the SA-PGA gel particles reached approximately 163.93 mg/g compared to the 81.97 mg/g adsorbed on SA alone.
25996388	8	44	theme	selective	971:979	arg1	adsorption					981:990	the selective adsorption	967:990	the selective adsorption of REEs on gel particles	967:1015	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	9	45	theme	porous	1320:1325	arg1	structure					1327:1335	a porous structure	1318:1335	a porous structure after doping PGA	1318:1352	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	9	46	from	groups	1173:1178	arg1	composite					1187:1195	the composite	1183:1195	the composite	1183:1195	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	8	47	from	solutions	936:944	arg1	types					894:898	15 types	891:898	15 types of REEs in single and mixed aqueous solutions	891:944	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	8	48	used	used	951:954	arg2	types					894:898	15 types	891:898	15 types of REEs in single and mixed aqueous solutions	891:944	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	8	49	theme	REEs	995:998	arg1	adsorption					981:990	the selective adsorption	967:990	the selective adsorption of REEs on gel particles	967:1015	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	9	50	theme	SA	1121:1122	arg1	analyses					1105:1112	infrared (FT-IR) spectroscopy analyses	1075:1112	infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads	1075:1143	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	9	51	theme	spectroscopy	1092:1103	arg1	analyses					1105:1112	infrared (FT-IR) spectroscopy analyses	1075:1112	infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads	1075:1143	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	9	52	theme	carboxyl	1164:1171	arg1	groups					1173:1178	the carboxyl groups	1160:1178	the carboxyl groups in the composite	1160:1195	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	9	53	theme	SA-PGA	1128:1133	arg1	beads					1139:1143	the SA and SA-PGA gel beads	1117:1143	beads	1139:1143	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	5	54	theme	excellent	488:496	arg1	capacity					498:505	excellent capacity	488:505	excellent capacity for adsorbing rare earth elements (REEs)	488:546	The composite exhibited excellent capacity for adsorbing rare earth elements (REEs).
25996388	7	55	theme	adsorbent	826:834	arg1	dosage					836:841	the adsorbent dosage	822:841	the adsorbent dosage	822:841	The factors that potentially affected the adsorption efficiency of the SA-PGA composite, including the initial concentration of REEs, the adsorbent dosage, and the pH of the solution, were investigated.
25996388	8	56	theme	REEs	903:906	arg1	types					894:898	15 types	891:898	15 types of REEs in single and mixed aqueous solutions	891:944	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	8	57	theme	gel	1003:1005	arg1	particles					1007:1015	gel particles	1003:1015	gel particles	1003:1015	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	4	58	theme	sodium	363:368	arg1	SA					380:381	SA	380:381	SA	380:381	Through doping sodium alginate (SA) with poly-γ-glutamate (PGA), an immobilized gel particle material was produced.
25996388	4	58	theme	sodium	363:368	arg1	alginate					370:377	sodium alginate	363:377	sodium alginate (SA)	363:382	Through doping sodium alginate (SA) with poly-γ-glutamate (PGA), an immobilized gel particle material was produced.
25996388	9	59	theme	electron	1027:1034	arg1	SEM					1048:1050	SEM	1048:1050	SEM	1048:1050	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	9	59	theme	electron	1027:1034	arg1	microscopy					1036:1045	Scanning electron microscopy	1018:1045	Scanning electron microscopy (SEM)	1018:1051	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	9	60	theme	doping	1343:1348	arg1	PGA					1350:1352	doping PGA	1343:1352	doping PGA	1343:1352	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	8	61	from	adsorption	981:990	arg1	particles					1007:1015	gel particles	1003:1015	gel particles	1003:1015	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	9	62	theme	gel	1135:1137	arg1	beads					1139:1143	the SA and SA-PGA gel beads	1117:1143	beads	1139:1143	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	2	63	theme	resources	212:220	arg1	This					192:195	This	192:195	This	192:195	This was a waste of resources and could be harmful to the organisms.
25996388	2	63	theme	resources	212:220	arg1	waste					203:207	a waste	201:207	a waste of resources	201:220	This was a waste of resources and could be harmful to the organisms.
25996388	8	64	from	REEs	903:906	arg1	solutions					936:944	single and mixed aqueous solutions	911:944	single and mixed aqueous solutions	911:944	15 types of REEs in single and mixed aqueous solutions were used to explore the selective adsorption of REEs on gel particles.
25996388	0	65	theme	Efficient	38:46	arg1	Hydrogel					64:71	an Efficient Sodium Alginate Hydrogel	35:71	an Efficient Sodium Alginate Hydrogel	35:71	Adsorption of Rare Earths(Ⅲ) Using an Efficient Sodium Alginate Hydrogel Cross-Linked with Poly-γ-Glutamate.
25996388	9	66	theme	beads	1139:1143	arg1	analyses					1105:1112	infrared (FT-IR) spectroscopy analyses	1075:1112	infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads	1075:1143	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
25996388	6	67	theme	81.97	655:659	arg1	mg/g					661:664	the 81.97 mg/g	651:664	the 81.97 mg/g adsorbed on SA alone	651:685	The amount of La3+ adsorbed on the SA-PGA gel particles reached approximately 163.93 mg/g compared to the 81.97 mg/g adsorbed on SA alone.
25996388	6	68	theme	La3+	563:566	arg1	amount					553:558	The amount	549:558	The amount of La3+ adsorbed on the SA-PGA gel particles	549:603	The amount of La3+ adsorbed on the SA-PGA gel particles reached approximately 163.93 mg/g compared to the 81.97 mg/g adsorbed on SA alone.
25996388	6	68	theme	La3+	563:566	arg1	La3+					563:566	La3+	563:566	La3+ adsorbed on the SA-PGA gel particles	563:603	The amount of La3+ adsorbed on the SA-PGA gel particles reached approximately 163.93 mg/g compared to the 81.97 mg/g adsorbed on SA alone.
25996388	4	69	theme	gel	428:430	arg1	material					441:448	an immobilized gel particle material	413:448	an immobilized gel particle material	413:448	Through doping sodium alginate (SA) with poly-γ-glutamate (PGA), an immobilized gel particle material was produced.
25996388	1	70	theme	rare	134:137	arg1	ore					145:147	rare earth ore	134:147	rare earth ore	134:147	With the exploitation of rare earth ore, more and more REEs came into groundwater.
25996388	9	71	dep	Fourier	1057:1063	arg1	transform					1065:1073	transform	1065:1073	transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads	1065:1143	Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy analyses of the SA and SA-PGA gel beads suggested that the carboxyl groups in the composite might play a key role in the adsorption process and the morphology of SA-PGA changed from the compact structure of SA to a porous structure after doping PGA.
27569576	5	0	theme	integration	908:918	arg1	scenario					920:927	the continuous fermentation-pervaporation integration scenario	866:927	the continuous fermentation-pervaporation integration scenario	866:927	Correspondingly, 1.6g/Lh, 7.8-9.8, 227.8-395g/m(2)h and 410.9-608.1g/L were achieved in the continuous fermentation-pervaporation integration scenario, respectively.
27569576	4	1	theme	ethanol	619:625	arg1	concentration					627:639	ethanol concentration	619:639	ethanol concentration	619:639	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	1	2	theme	fermentation-pervaporation	243:268	arg1	process					282:288	ethanol fermentation-pervaporation integration process	235:288	ethanol fermentation-pervaporation integration process	235:288	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane was used in ethanol fermentation-pervaporation integration process.
27569576	4	3	from	productivity	562:573	arg1	scenario					697:704	the fed-batch fermentation-pervaporation integration scenario	644:704	the fed-batch fermentation-pervaporation integration scenario	644:704	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	4	4	theme	319-416g/m	729:738	arg1	h					742:742	319-416g/m(2)h	729:742	319-416g/m(2)h	729:742	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	2	5	used	used	321:324	arg2	bagasse					309:315	The sweet sorghum bagasse	291:315	The sweet sorghum bagasse	291:315	The sweet sorghum bagasse was used as the immobilized carrier.
27569576	2	5	used	used	321:324	arg2	carrier					345:351	the immobilized carrier	329:351	the immobilized carrier	329:351	The sweet sorghum bagasse was used as the immobilized carrier.
27569576	1	6	theme	integration	270:280	arg1	process					282:288	ethanol fermentation-pervaporation integration process	235:288	ethanol fermentation-pervaporation integration process	235:288	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane was used in ethanol fermentation-pervaporation integration process.
27569576	4	7	theme	separation	576:585	arg1	factor					587:592	separation factor	576:592	separation factor	576:592	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	6	8	theme	integration	975:985	arg1	process					987:993	the integration process	971:993	the integration process	971:993	The results indicated that the integration process could greatly improve the ethanol production and separation performances.
27569576	4	9	theme	operations	538:547	arg1	long-term					525:533	long-term	525:533	long-term	525:533	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	3	10	theme	ethanol	464:470	arg1	productivity					472:483	higher ethanol productivity	457:483	higher ethanol productivity	457:483	Compared with the conventional suspend cells system, the immobilized fermentation system could provide higher ethanol productivity when coupled with pervaporation.
27569576	4	11	theme	total	595:599	arg1	flux					601:604	total flux	595:604	total flux	595:604	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	6	12	theme	separation	1044:1053	arg1	performances					1055:1066	separation performances	1044:1066	separation performances	1044:1066	The results indicated that the integration process could greatly improve the ethanol production and separation performances.
27569576	0	13	theme	ethanol	12:18	arg1	fermentation					20:31	Immobilized ethanol fermentation	0:31	Immobilized ethanol fermentation	0:31	Immobilized ethanol fermentation coupled to pervaporation with silicalite-1/polydimethylsiloxane/polyvinylidene fluoride composite membrane.
27569576	5	14	dep	h	828:828	arg1	2					826:826	2	826:826	2	826:826	Correspondingly, 1.6g/Lh, 7.8-9.8, 227.8-395g/m(2)h and 410.9-608.1g/L were achieved in the continuous fermentation-pervaporation integration scenario, respectively.
27569576	4	15	from	flux	601:604	arg1	scenario					697:704	the fed-batch fermentation-pervaporation integration scenario	644:704	the fed-batch fermentation-pervaporation integration scenario	644:704	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	5	16	theme	continuous	870:879	arg1	scenario					920:927	the continuous fermentation-pervaporation integration scenario	866:927	the continuous fermentation-pervaporation integration scenario	866:927	Correspondingly, 1.6g/Lh, 7.8-9.8, 227.8-395g/m(2)h and 410.9-608.1g/L were achieved in the continuous fermentation-pervaporation integration scenario, respectively.
27569576	1	17	used	used	227:230	arg2	membrane					214:221	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane	141:221	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane	141:221	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane was used in ethanol fermentation-pervaporation integration process.
27569576	5	18	theme	227.8-395g/m	813:824	arg1	h					828:828	227.8-395g/m(2)h	813:828	227.8-395g/m(2)h	813:828	Correspondingly, 1.6g/Lh, 7.8-9.8, 227.8-395g/m(2)h and 410.9-608.1g/L were achieved in the continuous fermentation-pervaporation integration scenario, respectively.
27569576	0	19	theme	Immobilized	0:10	arg1	fermentation					20:31	Immobilized ethanol fermentation	0:31	Immobilized ethanol fermentation	0:31	Immobilized ethanol fermentation coupled to pervaporation with silicalite-1/polydimethylsiloxane/polyvinylidene fluoride composite membrane.
27569576	4	20	theme	ethanol	554:560	arg1	1.6g/Lh					711:717	1.6g/Lh	711:717	1.6g/Lh	711:717	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	4	20	theme	ethanol	554:560	arg1	productivity					562:573	the ethanol productivity	550:573	the ethanol productivity	550:573	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	1	21	theme	novel	143:147	arg1	fluoride					198:205	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride	141:205	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane	141:221	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane was used in ethanol fermentation-pervaporation integration process.
27569576	4	22	from	1.6g/Lh	711:717	arg1	long-term					525:533	long-term	525:533	long-term	525:533	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	3	23	theme	cells	393:397	arg1	system					399:404	the conventional suspend cells system	368:404	the conventional suspend cells system	368:404	Compared with the conventional suspend cells system, the immobilized fermentation system could provide higher ethanol productivity when coupled with pervaporation.
27569576	1	24	theme	silicalite-1/polydimethylsiloxane/polyvinylidene	149:196	arg1	fluoride					198:205	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride	141:205	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane	141:221	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane was used in ethanol fermentation-pervaporation integration process.
27569576	4	25	from	factor	587:592	arg1	scenario					697:704	the fed-batch fermentation-pervaporation integration scenario	644:704	the fed-batch fermentation-pervaporation integration scenario	644:704	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	2	26	theme	sweet	295:299	arg1	bagasse					309:315	The sweet sorghum bagasse	291:315	The sweet sorghum bagasse	291:315	The sweet sorghum bagasse was used as the immobilized carrier.
27569576	2	26	theme	sweet	295:299	arg1	carrier					345:351	the immobilized carrier	329:351	the immobilized carrier	329:351	The sweet sorghum bagasse was used as the immobilized carrier.
27569576	1	27	theme	fluoride	198:205	arg1	membrane					214:221	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane	141:221	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane	141:221	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane was used in ethanol fermentation-pervaporation integration process.
27569576	3	28	theme	conventional	372:383	arg1	system					399:404	the conventional suspend cells system	368:404	the conventional suspend cells system	368:404	Compared with the conventional suspend cells system, the immobilized fermentation system could provide higher ethanol productivity when coupled with pervaporation.
27569576	6	29	theme	ethanol	1021:1027	arg1	production					1029:1038	the ethanol production	1017:1038	the ethanol production	1017:1038	The results indicated that the integration process could greatly improve the ethanol production and separation performances.
27569576	4	30	theme	fermentation-pervaporation	658:683	arg1	scenario					697:704	the fed-batch fermentation-pervaporation integration scenario	644:704	the fed-batch fermentation-pervaporation integration scenario	644:704	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	3	31	theme	suspend	385:391	arg1	system					399:404	the conventional suspend cells system	368:404	the conventional suspend cells system	368:404	Compared with the conventional suspend cells system, the immobilized fermentation system could provide higher ethanol productivity when coupled with pervaporation.
27569576	2	32	theme	immobilized	333:343	arg1	bagasse					309:315	The sweet sorghum bagasse	291:315	The sweet sorghum bagasse	291:315	The sweet sorghum bagasse was used as the immobilized carrier.
27569576	2	32	theme	immobilized	333:343	arg1	carrier					345:351	the immobilized carrier	329:351	the immobilized carrier	329:351	The sweet sorghum bagasse was used as the immobilized carrier.
27569576	0	33	theme	silicalite-1/polydimethylsiloxane/polyvinylidene	63:110	arg1	membrane					131:138	silicalite-1/polydimethylsiloxane/polyvinylidene fluoride composite membrane	63:138	silicalite-1/polydimethylsiloxane/polyvinylidene fluoride composite membrane	63:138	Immobilized ethanol fermentation coupled to pervaporation with silicalite-1/polydimethylsiloxane/polyvinylidene fluoride composite membrane.
27569576	4	34	theme	fed-batch	648:656	arg1	scenario					697:704	the fed-batch fermentation-pervaporation integration scenario	644:704	the fed-batch fermentation-pervaporation integration scenario	644:704	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	3	35	theme	immobilized	411:421	arg1	system					436:441	the immobilized fermentation system	407:441	the immobilized fermentation system	407:441	Compared with the conventional suspend cells system, the immobilized fermentation system could provide higher ethanol productivity when coupled with pervaporation.
27569576	0	36	theme	fluoride	112:119	arg1	membrane					131:138	silicalite-1/polydimethylsiloxane/polyvinylidene fluoride composite membrane	63:138	silicalite-1/polydimethylsiloxane/polyvinylidene fluoride composite membrane	63:138	Immobilized ethanol fermentation coupled to pervaporation with silicalite-1/polydimethylsiloxane/polyvinylidene fluoride composite membrane.
27569576	1	37	theme	hybrid	207:212	arg1	membrane					214:221	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane	141:221	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane	141:221	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane was used in ethanol fermentation-pervaporation integration process.
27569576	3	38	theme	fermentation	423:434	arg1	system					436:441	the immobilized fermentation system	407:441	the immobilized fermentation system	407:441	Compared with the conventional suspend cells system, the immobilized fermentation system could provide higher ethanol productivity when coupled with pervaporation.
27569576	4	39	theme	integration	685:695	arg1	scenario					697:704	the fed-batch fermentation-pervaporation integration scenario	644:704	the fed-batch fermentation-pervaporation integration scenario	644:704	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	5	40	theme	fermentation-pervaporation	881:906	arg1	scenario					920:927	the continuous fermentation-pervaporation integration scenario	866:927	the continuous fermentation-pervaporation integration scenario	866:927	Correspondingly, 1.6g/Lh, 7.8-9.8, 227.8-395g/m(2)h and 410.9-608.1g/L were achieved in the continuous fermentation-pervaporation integration scenario, respectively.
27569576	4	41	dep	productivity	562:573	arg1	concentration					627:639	ethanol concentration	619:639	ethanol concentration	619:639	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	4	42	dep	h	742:742	arg1	2					740:740	2	740:740	2	740:740	In the long-term of operations, the ethanol productivity, separation factor, total flux and permeate ethanol concentration in the fed-batch fermentation-pervaporation integration scenario were 1.6g/Lh, 8.2-9.9, 319-416g/m(2)h and 426.9-597.2g/L, respectively.
27569576	0	43	theme	composite	121:129	arg1	membrane					131:138	silicalite-1/polydimethylsiloxane/polyvinylidene fluoride composite membrane	63:138	silicalite-1/polydimethylsiloxane/polyvinylidene fluoride composite membrane	63:138	Immobilized ethanol fermentation coupled to pervaporation with silicalite-1/polydimethylsiloxane/polyvinylidene fluoride composite membrane.
27569576	2	44	theme	sorghum	301:307	arg1	bagasse					309:315	The sweet sorghum bagasse	291:315	The sweet sorghum bagasse	291:315	The sweet sorghum bagasse was used as the immobilized carrier.
27569576	2	44	theme	sorghum	301:307	arg1	carrier					345:351	the immobilized carrier	329:351	the immobilized carrier	329:351	The sweet sorghum bagasse was used as the immobilized carrier.
27569576	3	45	theme	higher	457:462	arg1	productivity					472:483	higher ethanol productivity	457:483	higher ethanol productivity	457:483	Compared with the conventional suspend cells system, the immobilized fermentation system could provide higher ethanol productivity when coupled with pervaporation.
27569576	1	46	theme	ethanol	235:241	arg1	process					282:288	ethanol fermentation-pervaporation integration process	235:288	ethanol fermentation-pervaporation integration process	235:288	A novel silicalite-1/polydimethylsiloxane/polyvinylidene fluoride hybrid membrane was used in ethanol fermentation-pervaporation integration process.
27474642	1	0	theme	RC-4	178:181	arg1	membrane					183:190	regenerated cellulose (RC-4 membrane)	155:191	regenerated cellulose (RC-4 membrane)	155:191	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	5	1	from	environments	1058:1069	arg1	effect					1081:1086	their effect	1075:1086	their effect on diffusive transport	1075:1109	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	5	1	from	environments	1058:1069	arg1	structure					1011:1019	the structure	1007:1019	the structure of the cellulosic support in aqueous environments	1007:1069	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	1	2	theme	silicon	262:268	arg1	SiQDs					297:301	SiQDs	297:301	SiQDs	297:301	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	1	2	theme	silicon	262:268	arg1	nanoparticles					282:294	water-soluble silicon quantum dot nanoparticles	248:294	water-soluble silicon quantum dot nanoparticles (SiQDs)	248:302	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	5	3	theme	nanoparticles	990:1002	arg1	permanence					970:979	the permanence	966:979	the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport	966:1109	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	4	4	theme	such	942:945	arg1	effects					947:953	such effects	942:953	such effects	942:953	According to these results, both SiQDs-regenerated cellulose composite membranes present luminescent character as well as higher thermal resistance and conductivity than the original sample, although the dendrimer coverage of the SiQDs might partially shield such effects.
27474642	5	5	from	permanence	970:979	arg1	effect					1081:1086	their effect	1075:1086	their effect on diffusive transport	1075:1109	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	5	5	from	permanence	970:979	arg1	transport					1101:1109	diffusive transport	1091:1109	diffusive transport	1091:1109	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	5	5	from	permanence	970:979	arg1	structure					1011:1019	the structure	1007:1019	the structure of the cellulosic support in aqueous environments	1007:1069	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	2	6	theme	PAMAM-OH	345:352	arg1	dendrimer					354:362	a PAMAM-OH dendrimer	343:362	a PAMAM-OH dendrimer	343:362	Both bare SiQDs and SiQDs coated with a PAMAM-OH dendrimer were employed in order to obtain luminescent and thermally stable membrane systems (RC-4/SiQDs and RC-4/SiQDs-PAMAM-OH membranes).
27474642	5	7	from	support	1039:1045	arg1	environments					1058:1069	aqueous environments	1050:1069	aqueous environments	1050:1069	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	1	8	theme	quantum	270:276	arg1	SiQDs					297:301	SiQDs	297:301	SiQDs	297:301	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	1	8	theme	quantum	270:276	arg1	nanoparticles					282:294	water-soluble silicon quantum dot nanoparticles	248:294	water-soluble silicon quantum dot nanoparticles (SiQDs)	248:302	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	5	9	theme	model	1222:1226	arg1	KCl					1241:1243	a model electrolyte (KCl)	1220:1244	a model electrolyte (KCl)	1220:1244	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	6	10	theme	SiQDs	1352:1356	arg1	nanoparticles					1358:1370	SiQDs nanoparticles	1352:1370	SiQDs nanoparticles	1352:1370	These results demonstrate the possible use of these stable nano-engineered membranes, which are based on SiQDs nanoparticles, in electrochemical devices under flow conditions.
27474642	6	11	theme	stable	1299:1304	arg1	membranes					1322:1330	these stable nano-engineered membranes	1293:1330	these stable nano-engineered membranes	1293:1330	These results demonstrate the possible use of these stable nano-engineered membranes, which are based on SiQDs nanoparticles, in electrochemical devices under flow conditions.
27474642	5	12	theme	support	1039:1045	arg1	effect					1081:1086	their effect	1075:1086	their effect on diffusive transport	1075:1109	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	5	12	theme	support	1039:1045	arg1	structure					1011:1019	the structure	1007:1019	the structure of the cellulosic support in aqueous environments	1007:1069	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	5	13	theme	cellulosic	1028:1037	arg1	support					1039:1045	the cellulosic support	1024:1045	the cellulosic support in aqueous environments	1024:1069	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	1	14	theme	dot	278:280	arg1	SiQDs					297:301	SiQDs	297:301	SiQDs	297:301	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	1	14	theme	dot	278:280	arg1	nanoparticles					282:294	water-soluble silicon quantum dot nanoparticles	248:294	water-soluble silicon quantum dot nanoparticles (SiQDs)	248:302	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	5	15	theme	potential	1166:1174	arg1	measurements					1176:1187	membrane potential measurements	1157:1187	membrane potential measurements at different concentrations of a model electrolyte (KCl)	1157:1244	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	4	16	theme	luminescent	772:782	arg1	character					784:792	luminescent character	772:792	luminescent character as well as higher thermal resistance and conductivity	772:846	According to these results, both SiQDs-regenerated cellulose composite membranes present luminescent character as well as higher thermal resistance and conductivity than the original sample, although the dendrimer coverage of the SiQDs might partially shield such effects.
27474642	5	17	from	transport	1101:1109	arg1	permanence					970:979	the permanence	966:979	the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport	966:1109	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	1	18	theme	nanoparticles	282:294	arg1	inclusion					235:243	inclusion	235:243	inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs)	235:302	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	5	19	from	effect	1081:1086	arg1	transport					1101:1109	diffusive transport	1091:1109	diffusive transport	1091:1109	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	5	19	from	effect	1081:1086	arg1	environments					1058:1069	aqueous environments	1050:1069	aqueous environments	1050:1069	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	2	20	dep	systems	439:445	arg1	RC-4/SiQDs					448:457	RC-4/SiQDs	448:457	RC-4/SiQDs	448:457	Both bare SiQDs and SiQDs coated with a PAMAM-OH dendrimer were employed in order to obtain luminescent and thermally stable membrane systems (RC-4/SiQDs and RC-4/SiQDs-PAMAM-OH membranes).
27474642	2	20	dep	systems	439:445	arg1	membranes					483:491	RC-4/SiQDs-PAMAM-OH membranes	463:491	RC-4/SiQDs-PAMAM-OH membranes	463:491	Both bare SiQDs and SiQDs coated with a PAMAM-OH dendrimer were employed in order to obtain luminescent and thermally stable membrane systems (RC-4/SiQDs and RC-4/SiQDs-PAMAM-OH membranes).
27474642	3	21	theme	fluorescence	555:566	arg1	spectroscopy					568:579	fluorescence spectroscopy	555:579	fluorescence spectroscopy (steady and confocal)	555:601	Original and SiQDs-modified membranes were characterized by fluorescence spectroscopy (steady and confocal), derivative thermogravimetric analysis and impedance spectroscopy measurements.
27474642	5	22	theme	membrane	1157:1164	arg1	measurements					1176:1187	membrane potential measurements	1157:1187	membrane potential measurements at different concentrations of a model electrolyte (KCl)	1157:1244	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	6	23	from	use	1286:1288	arg1	devices					1392:1398	electrochemical devices	1376:1398	electrochemical devices	1376:1398	These results demonstrate the possible use of these stable nano-engineered membranes, which are based on SiQDs nanoparticles, in electrochemical devices under flow conditions.
27474642	4	24	theme	SiQDs	913:917	arg1	coverage					897:904	the dendrimer coverage	883:904	the dendrimer coverage of the SiQDs	883:917	According to these results, both SiQDs-regenerated cellulose composite membranes present luminescent character as well as higher thermal resistance and conductivity than the original sample, although the dendrimer coverage of the SiQDs might partially shield such effects.
27474642	5	25	theme	different	1192:1200	arg1	concentrations					1202:1215	different concentrations	1192:1215	different concentrations of a model electrolyte (KCl)	1192:1244	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	4	26	theme	thermal	812:818	arg1	resistance					820:829	higher thermal resistance	805:829	higher thermal resistance	805:829	According to these results, both SiQDs-regenerated cellulose composite membranes present luminescent character as well as higher thermal resistance and conductivity than the original sample, although the dendrimer coverage of the SiQDs might partially shield such effects.
27474642	4	27	theme	cellulose	734:742	arg1	membranes					754:762	both SiQDs-regenerated cellulose composite membranes	711:762	both SiQDs-regenerated cellulose composite membranes	711:762	According to these results, both SiQDs-regenerated cellulose composite membranes present luminescent character as well as higher thermal resistance and conductivity than the original sample, although the dendrimer coverage of the SiQDs might partially shield such effects.
27474642	4	28	theme	higher	805:810	arg1	resistance					820:829	higher thermal resistance	805:829	higher thermal resistance	805:829	According to these results, both SiQDs-regenerated cellulose composite membranes present luminescent character as well as higher thermal resistance and conductivity than the original sample, although the dendrimer coverage of the SiQDs might partially shield such effects.
27474642	1	29	theme	biocompatible	196:208	arg1	membrane					130:137	A highly hydrophilic planar membrane	102:137	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane)	102:191	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	1	29	theme	biocompatible	196:208	arg1	polymer					210:216	a biocompatible polymer	194:216	a biocompatible polymer	194:216	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	0	30	theme	membranes	30:38	arg1	Characterization					0:15	Characterization	0:15	Characterization of cellulose membranes modified with luminescent silicon quantum dots	0:85	Characterization of cellulose membranes modified with luminescent silicon quantum dots nanoparticles.
27474642	6	31	theme	possible	1277:1284	arg1	use					1286:1288	the possible use	1273:1288	the possible use of these stable nano-engineered membranes, which are based on SiQDs nanoparticles, in electrochemical devices	1273:1398	These results demonstrate the possible use of these stable nano-engineered membranes, which are based on SiQDs nanoparticles, in electrochemical devices under flow conditions.
27474642	1	32	theme	hydrophilic	111:121	arg1	membrane					130:137	A highly hydrophilic planar membrane	102:137	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane)	102:191	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	1	32	theme	hydrophilic	111:121	arg1	polymer					210:216	a biocompatible polymer	194:216	a biocompatible polymer	194:216	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	6	33	theme	membranes	1322:1330	arg1	use					1286:1288	the possible use	1273:1288	the possible use of these stable nano-engineered membranes, which are based on SiQDs nanoparticles, in electrochemical devices	1273:1398	These results demonstrate the possible use of these stable nano-engineered membranes, which are based on SiQDs nanoparticles, in electrochemical devices under flow conditions.
27474642	2	34	theme	stable	423:428	arg1	systems					439:445	luminescent and thermally stable membrane systems	397:445	luminescent and thermally stable membrane systems (RC-4/SiQDs and RC-4/SiQDs-PAMAM-OH membranes)	397:492	Both bare SiQDs and SiQDs coated with a PAMAM-OH dendrimer were employed in order to obtain luminescent and thermally stable membrane systems (RC-4/SiQDs and RC-4/SiQDs-PAMAM-OH membranes).
27474642	2	35	theme	membrane	430:437	arg1	systems					439:445	luminescent and thermally stable membrane systems	397:445	luminescent and thermally stable membrane systems (RC-4/SiQDs and RC-4/SiQDs-PAMAM-OH membranes)	397:492	Both bare SiQDs and SiQDs coated with a PAMAM-OH dendrimer were employed in order to obtain luminescent and thermally stable membrane systems (RC-4/SiQDs and RC-4/SiQDs-PAMAM-OH membranes).
27474642	4	36	theme	original	857:864	arg1	sample					866:871	the original sample	853:871	the original sample	853:871	According to these results, both SiQDs-regenerated cellulose composite membranes present luminescent character as well as higher thermal resistance and conductivity than the original sample, although the dendrimer coverage of the SiQDs might partially shield such effects.
27474642	1	37	theme	planar	123:128	arg1	membrane					130:137	A highly hydrophilic planar membrane	102:137	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane)	102:191	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	1	37	theme	planar	123:128	arg1	polymer					210:216	a biocompatible polymer	194:216	a biocompatible polymer	194:216	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	1	38	mod	modified	223:230	arg1	membrane					130:137	A highly hydrophilic planar membrane	102:137	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane)	102:191	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	1	38	mod	modified	223:230	arg3	inclusion					235:243	inclusion	235:243	inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs)	235:302	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	1	38	mod	modified	223:230	arg1	polymer					210:216	a biocompatible polymer	194:216	a biocompatible polymer	194:216	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	3	39	dep	spectroscopy	568:579	arg1	confocal					593:600	confocal	593:600	confocal	593:600	Original and SiQDs-modified membranes were characterized by fluorescence spectroscopy (steady and confocal), derivative thermogravimetric analysis and impedance spectroscopy measurements.
27474642	3	39	dep	spectroscopy	568:579	arg1	steady					582:587	steady	582:587	steady	582:587	Original and SiQDs-modified membranes were characterized by fluorescence spectroscopy (steady and confocal), derivative thermogravimetric analysis and impedance spectroscopy measurements.
27474642	3	39	dep	spectroscopy	568:579	arg1	measurements					669:680	measurements	669:680	measurements	669:680	Original and SiQDs-modified membranes were characterized by fluorescence spectroscopy (steady and confocal), derivative thermogravimetric analysis and impedance spectroscopy measurements.
27474642	5	40	from	concentrations	1202:1215	arg1	measurements					1176:1187	membrane potential measurements	1157:1187	membrane potential measurements at different concentrations of a model electrolyte (KCl)	1157:1244	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	5	41	theme	diffusive	1091:1099	arg1	transport					1101:1109	diffusive transport	1091:1109	diffusive transport	1091:1109	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	4	42	theme	SiQDs-regenerated	716:732	arg1	membranes					754:762	both SiQDs-regenerated cellulose composite membranes	711:762	both SiQDs-regenerated cellulose composite membranes	711:762	According to these results, both SiQDs-regenerated cellulose composite membranes present luminescent character as well as higher thermal resistance and conductivity than the original sample, although the dendrimer coverage of the SiQDs might partially shield such effects.
27474642	5	43	theme	electrolyte	1228:1238	arg1	KCl					1241:1243	a model electrolyte (KCl)	1220:1244	a model electrolyte (KCl)	1220:1244	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	6	44	theme	flow	1406:1409	arg1	conditions					1411:1420	flow conditions	1406:1420	flow conditions	1406:1420	These results demonstrate the possible use of these stable nano-engineered membranes, which are based on SiQDs nanoparticles, in electrochemical devices under flow conditions.
27474642	4	45	theme	dendrimer	887:895	arg1	coverage					897:904	the dendrimer coverage	883:904	the dendrimer coverage of the SiQDs	883:917	According to these results, both SiQDs-regenerated cellulose composite membranes present luminescent character as well as higher thermal resistance and conductivity than the original sample, although the dendrimer coverage of the SiQDs might partially shield such effects.
27474642	2	46	theme	luminescent	397:407	arg1	systems					439:445	luminescent and thermally stable membrane systems	397:445	luminescent and thermally stable membrane systems (RC-4/SiQDs and RC-4/SiQDs-PAMAM-OH membranes)	397:492	Both bare SiQDs and SiQDs coated with a PAMAM-OH dendrimer were employed in order to obtain luminescent and thermally stable membrane systems (RC-4/SiQDs and RC-4/SiQDs-PAMAM-OH membranes).
27474642	2	47	theme	RC-4/SiQDs-PAMAM-OH	463:481	arg1	membranes					483:491	RC-4/SiQDs-PAMAM-OH membranes	463:491	RC-4/SiQDs-PAMAM-OH membranes	463:491	Both bare SiQDs and SiQDs coated with a PAMAM-OH dendrimer were employed in order to obtain luminescent and thermally stable membrane systems (RC-4/SiQDs and RC-4/SiQDs-PAMAM-OH membranes).
27474642	0	48	theme	quantum	74:80	arg1	dots					82:85	luminescent silicon quantum dots	54:85	luminescent silicon quantum dots	54:85	Characterization of cellulose membranes modified with luminescent silicon quantum dots nanoparticles.
27474642	3	49	theme	derivative	604:613	arg1	analysis					633:640	derivative thermogravimetric analysis	604:640	derivative thermogravimetric analysis	604:640	Original and SiQDs-modified membranes were characterized by fluorescence spectroscopy (steady and confocal), derivative thermogravimetric analysis and impedance spectroscopy measurements.
27474642	5	50	theme	SiQDs	984:988	arg1	nanoparticles					990:1002	SiQDs nanoparticles	984:1002	SiQDs nanoparticles	984:1002	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	0	51	theme	luminescent	54:64	arg1	dots					82:85	luminescent silicon quantum dots	54:85	luminescent silicon quantum dots	54:85	Characterization of cellulose membranes modified with luminescent silicon quantum dots nanoparticles.
27474642	5	52	theme	KCl	1241:1243	arg1	concentrations					1202:1215	different concentrations	1192:1215	different concentrations of a model electrolyte (KCl)	1192:1244	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	3	53	theme	thermogravimetric	615:631	arg1	analysis					633:640	derivative thermogravimetric analysis	604:640	derivative thermogravimetric analysis	604:640	Original and SiQDs-modified membranes were characterized by fluorescence spectroscopy (steady and confocal), derivative thermogravimetric analysis and impedance spectroscopy measurements.
27474642	3	54	theme	SiQDs-modified	508:521	arg1	membranes					523:531	Original and SiQDs-modified membranes	495:531	Original and SiQDs-modified membranes	495:531	Original and SiQDs-modified membranes were characterized by fluorescence spectroscopy (steady and confocal), derivative thermogravimetric analysis and impedance spectroscopy measurements.
27474642	6	55	theme	electrochemical	1376:1390	arg1	devices					1392:1398	electrochemical devices	1376:1398	electrochemical devices	1376:1398	These results demonstrate the possible use of these stable nano-engineered membranes, which are based on SiQDs nanoparticles, in electrochemical devices under flow conditions.
27474642	5	56	theme	water	1130:1134	arg1	uptake					1136:1141	water uptake	1130:1141	water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl)	1130:1244	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	6	57	theme	nano-engineered	1306:1320	arg1	membranes					1322:1330	these stable nano-engineered membranes	1293:1330	these stable nano-engineered membranes	1293:1330	These results demonstrate the possible use of these stable nano-engineered membranes, which are based on SiQDs nanoparticles, in electrochemical devices under flow conditions.
27474642	2	58	theme	bare	310:313	arg1	SiQDs					315:319	Both bare SiQDs	305:319	Both bare SiQDs	305:319	Both bare SiQDs and SiQDs coated with a PAMAM-OH dendrimer were employed in order to obtain luminescent and thermally stable membrane systems (RC-4/SiQDs and RC-4/SiQDs-PAMAM-OH membranes).
27474642	1	59	theme	regenerated	155:165	arg1	membrane					183:190	regenerated cellulose (RC-4 membrane)	155:191	regenerated cellulose (RC-4 membrane)	155:191	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	5	60	from	structure	1011:1019	arg1	transport					1101:1109	diffusive transport	1091:1109	diffusive transport	1091:1109	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	5	60	from	structure	1011:1019	arg1	environments					1058:1069	aqueous environments	1050:1069	aqueous environments	1050:1069	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	5	61	theme	aqueous	1050:1056	arg1	environments					1058:1069	aqueous environments	1050:1069	aqueous environments	1050:1069	Moreover, the permanence of SiQDs nanoparticles in the structure of the cellulosic support in aqueous environments and their effect on diffusive transport were determined by water uptake as well as by membrane potential measurements at different concentrations of a model electrolyte (KCl).
27474642	3	62	theme	impedance	646:654	arg1	spectroscopy					656:667	impedance spectroscopy	646:667	impedance spectroscopy	646:667	Original and SiQDs-modified membranes were characterized by fluorescence spectroscopy (steady and confocal), derivative thermogravimetric analysis and impedance spectroscopy measurements.
27474642	1	63	theme	cellulose	167:175	arg1	membrane					183:190	regenerated cellulose (RC-4 membrane)	155:191	regenerated cellulose (RC-4 membrane)	155:191	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	3	64	theme	Original	495:502	arg1	membranes					523:531	Original and SiQDs-modified membranes	495:531	Original and SiQDs-modified membranes	495:531	Original and SiQDs-modified membranes were characterized by fluorescence spectroscopy (steady and confocal), derivative thermogravimetric analysis and impedance spectroscopy measurements.
27474642	0	65	theme	silicon	66:72	arg1	dots					82:85	luminescent silicon quantum dots	54:85	luminescent silicon quantum dots	54:85	Characterization of cellulose membranes modified with luminescent silicon quantum dots nanoparticles.
27474642	0	66	theme	cellulose	20:28	arg1	membranes					30:38	cellulose membranes	20:38	cellulose membranes modified with luminescent silicon quantum dots	20:85	Characterization of cellulose membranes modified with luminescent silicon quantum dots nanoparticles.
27474642	4	67	theme	composite	744:752	arg1	membranes					754:762	both SiQDs-regenerated cellulose composite membranes	711:762	both SiQDs-regenerated cellulose composite membranes	711:762	According to these results, both SiQDs-regenerated cellulose composite membranes present luminescent character as well as higher thermal resistance and conductivity than the original sample, although the dendrimer coverage of the SiQDs might partially shield such effects.
27474642	1	68	theme	water-soluble	248:260	arg1	SiQDs					297:301	SiQDs	297:301	SiQDs	297:301	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
27474642	1	68	theme	water-soluble	248:260	arg1	nanoparticles					282:294	water-soluble silicon quantum dot nanoparticles	248:294	water-soluble silicon quantum dot nanoparticles (SiQDs)	248:302	A highly hydrophilic planar membrane fabricated with regenerated cellulose (RC-4 membrane), a biocompatible polymer, was modified by inclusion of water-soluble silicon quantum dot nanoparticles (SiQDs).
25428893	0	0	theme	O-antigen	65:73	arg1	cluster					93:99	the Escherichia coli O-antigen biosynthesis gene cluster	44:99	the Escherichia coli O-antigen biosynthesis gene cluster	44:99	A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.
25428893	6	1	theme	serogroups	836:845	arg1	one-quarter					813:823	the nearly one-quarter	802:823	the nearly one-quarter of the 184 serogroups	802:845	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	2	2	theme	serological	341:351	arg1	diversity					353:361	serological diversity	341:361	serological diversity	341:361	The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity.
25428893	7	3	from	view	997:1000	arg1	coli					1043:1046	E. coli	1040:1046	E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized	1040:1170	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
25428893	8	4	attach	linked	1315:1320	arg2	approaches					1298:1307	traditional typing approaches	1279:1307	traditional typing approaches	1279:1307	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	8	4	attach	linked	1315:1320	arg1	exploration					1333:1343	genomic exploration	1325:1343	genomic exploration of E. coli diversity	1325:1364	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	6	5	theme	ST10	865:868	arg1	lineage					870:876	the ST10 lineage	861:876	the ST10 lineage	861:876	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	7	6	theme	diversity	1017:1025	arg1	view					997:1000	a complete view	986:1000	a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized	986:1170	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
25428893	1	7	from	part	142:145	arg1	bacteria					196:203	Gram-negative bacteria	182:203	Gram-negative bacteria	182:203	The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria.
25428893	3	8	theme	Escherichia	483:493	arg1	serogroups					502:511	all 184 recognized Escherichia coli O serogroups	464:511	all 184 recognized Escherichia coli O serogroups	464:511	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	6	9	theme	successful	938:947	arg1	exchange					949:956	a more successful exchange	931:956	a more successful exchange of O-AGCs	931:966	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	0	10	theme	gene	88:91	arg1	cluster					93:99	the Escherichia coli O-antigen biosynthesis gene cluster	44:99	the Escherichia coli O-antigen biosynthesis gene cluster	44:99	A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.
25428893	6	11	theme	reference	770:778	arg1	strains					780:786	all the E. coli O-serogroup reference strains	742:786	all the E. coli O-serogroup reference strains	742:786	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	0	12	theme	biosynthesis	75:86	arg1	cluster					93:99	the Escherichia coli O-antigen biosynthesis gene cluster	44:99	the Escherichia coli O-antigen biosynthesis gene cluster	44:99	A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.
25428893	6	13	theme	O-serogroup	758:768	arg1	strains					780:786	all the E. coli O-serogroup reference strains	742:786	all the E. coli O-serogroup reference strains	742:786	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	3	14	theme	recognized	472:481	arg1	serogroups					502:511	all 184 recognized Escherichia coli O serogroups	464:511	all 184 recognized Escherichia coli O serogroups	464:511	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	4	15	theme	well-defined	562:573	arg1	O-AGCs					575:580	161 well-defined O-AGCs	558:580	161 well-defined O-AGCs	558:580	By comparing these sequences, we identified 161 well-defined O-AGCs.
25428893	7	16	theme	O-AGCs	1030:1035	arg1	diversity					1017:1025	the genetic diversity	1005:1025	the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized	1005:1170	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
25428893	8	17	theme	molecular	1237:1245	arg1	scheme					1256:1261	a systematic molecular O-typing scheme	1224:1261	a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity	1224:1364	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	2	18	theme	high	267:270	arg1	levels					272:277	high levels	267:277	high levels of variation	267:290	The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity.
25428893	3	19	theme	O-antigen	413:421	arg1	clusters					441:448	the O-antigen biosynthesis gene clusters	409:448	the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups	409:511	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	3	19	theme	O-antigen	413:421	arg1	O-AGCs					451:456	O-AGCs	451:456	O-AGCs	451:456	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	0	20	theme	cluster	93:99	arg1	diversity					31:39	the genetic diversity	19:39	the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster	19:99	A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.
25428893	3	21	theme	sequence	392:399	arg1	set					401:403	a complete sequence set	381:403	a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups	381:511	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	6	22	theme	E.	750:751	arg1	strains					780:786	all the E. coli O-serogroup reference strains	742:786	all the E. coli O-serogroup reference strains	742:786	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	6	23	dep	E.	750:751	arg1	coli					753:756	coli	753:756	coli	753:756	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	1	24	theme	lipopolysaccharide	154:171	arg1	layer					173:177	the lipopolysaccharide layer	150:177	the lipopolysaccharide layer in Gram-negative bacteria	150:203	The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria.
25428893	0	25	theme	complete	2:9	arg1	view					11:14	A complete view	0:14	A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster	0:99	A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.
25428893	3	26	theme	O	500:500	arg1	serogroups					502:511	all 184 recognized Escherichia coli O serogroups	464:511	all 184 recognized Escherichia coli O serogroups	464:511	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	5	27	theme	wzm/wzt	607:613	arg1	sequences					620:628	the wzx/wzy or wzm/wzt gene sequences	592:628	sequences	620:628	Based on the wzx/wzy or wzm/wzt gene sequences, in addition to 145 singletons, 37 serogroups were placed into 16 groups.
25428893	3	28	theme	complete	383:390	arg1	set					401:403	a complete sequence set	381:403	a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups	381:511	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	1	29	theme	layer	173:177	arg1	part					142:145	the outermost part	128:145	the outermost part of the lipopolysaccharide layer in Gram-negative bacteria	128:203	The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria.
25428893	7	30	theme	O-serogroup	1117:1127	arg1	diversification					1129:1143	O-serogroup diversification	1117:1143	O-serogroup diversification	1117:1143	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
25428893	7	31	from	diversity	1017:1025	arg1	coli					1043:1046	E. coli	1040:1046	E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized	1040:1170	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
25428893	5	32	theme	gene	615:618	arg1	sequences					620:628	the wzx/wzy or wzm/wzt gene sequences	592:628	sequences	620:628	Based on the wzx/wzy or wzm/wzt gene sequences, in addition to 145 singletons, 37 serogroups were placed into 16 groups.
25428893	3	33	dep	Escherichia	483:493	arg1	coli					495:498	coli	495:498	coli	495:498	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	7	34	dep	lineage	1105:1111	arg1	recognized					1161:1170	recognized	1161:1170	recognized	1161:1170	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
25428893	7	35	theme	phylogenetic	1092:1103	arg1	lineage					1105:1111	host phylogenetic lineage	1087:1111	host phylogenetic lineage	1087:1111	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
25428893	8	36	theme	diversity	1356:1364	arg1	exploration					1333:1343	genomic exploration	1325:1343	genomic exploration of E. coli diversity	1325:1364	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	0	37	dep	Escherichia	48:58	arg1	coli					60:63	coli	60:63	coli	60:63	A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.
25428893	6	38	theme	strains	780:786	arg1	analysis					730:737	phylogenetic analysis	717:737	phylogenetic analysis of all the E. coli O-serogroup reference strains	717:786	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	1	39	theme	Gram-negative	182:194	arg1	bacteria					196:203	Gram-negative bacteria	182:203	Gram-negative bacteria	182:203	The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria.
25428893	8	40	theme	genomic	1325:1331	arg1	exploration					1333:1343	genomic exploration	1325:1343	genomic exploration of E. coli diversity	1325:1364	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	6	41	theme	genetic	903:909	arg1	background					911:920	a unique genetic background	894:920	a unique genetic background allowing a more successful exchange of O-AGCs	894:966	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	7	42	theme	genetic	1009:1015	arg1	diversity					1017:1025	the genetic diversity	1005:1025	the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized	1005:1170	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
25428893	2	43	theme	variation	282:290	arg1	levels					272:277	high levels	267:277	high levels of variation	267:290	The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity.
25428893	0	44	theme	genetic	23:29	arg1	diversity					31:39	the genetic diversity	19:39	the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster	19:99	A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.
25428893	6	45	theme	unique	896:901	arg1	background					911:920	a unique genetic background	894:920	a unique genetic background allowing a more successful exchange of O-AGCs	894:966	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	8	46	theme	systematic	1226:1235	arg1	scheme					1256:1261	a systematic molecular O-typing scheme	1224:1261	a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity	1224:1364	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	8	47	theme	coli	1351:1354	arg1	diversity					1356:1364	E. coli diversity	1348:1364	E. coli diversity	1348:1364	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	8	48	theme	valuable	1194:1201	arg1	basis					1203:1207	a valuable basis	1192:1207	a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity	1192:1364	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	8	48	theme	valuable	1194:1201	arg1	data					1179:1182	These data	1173:1182	These data	1173:1182	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	3	49	theme	gene	436:439	arg1	clusters					441:448	the O-antigen biosynthesis gene clusters	409:448	the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups	409:511	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	3	49	theme	gene	436:439	arg1	O-AGCs					451:456	O-AGCs	451:456	O-AGCs	451:456	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	6	50	located	found	852:856	arg1	lineage					870:876	the ST10 lineage	861:876	the ST10 lineage	861:876	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	6	50	located	found	852:856	arg2	one-quarter					813:823	the nearly one-quarter	802:823	the nearly one-quarter of the 184 serogroups	802:845	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	7	51	theme	host	1087:1090	arg1	lineage					1105:1111	host phylogenetic lineage	1087:1111	host phylogenetic lineage	1087:1111	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
25428893	8	52	theme	E.	1348:1349	arg1	diversity					1356:1364	E. coli diversity	1348:1364	E. coli diversity	1348:1364	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	7	53	theme	stronger	1058:1065	arg1	association					1067:1077	a stronger association	1056:1077	a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized	1056:1170	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
25428893	5	54	dep	singletons	650:659	arg1	addition					634:641	addition	634:641	addition	634:641	Based on the wzx/wzy or wzm/wzt gene sequences, in addition to 145 singletons, 37 serogroups were placed into 16 groups.
25428893	6	55	theme	phylogenetic	717:728	arg1	analysis					730:737	phylogenetic analysis	717:737	phylogenetic analysis of all the E. coli O-serogroup reference strains	717:786	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	8	56	theme	O-typing	1247:1254	arg1	scheme					1256:1261	a systematic molecular O-typing scheme	1224:1261	a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity	1224:1364	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	0	57	theme	diversity	31:39	arg1	view					11:14	A complete view	0:14	A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster	0:99	A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.
25428893	7	58	theme	complete	988:995	arg1	view					997:1000	a complete view	986:1000	a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized	986:1170	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
25428893	8	59	theme	traditional	1279:1289	arg1	approaches					1298:1307	traditional typing approaches	1279:1307	traditional typing approaches	1279:1307	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	2	60	theme	chemical	210:217	arg1	composition					219:229	chemical composition	210:229	chemical composition	210:229	The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity.
25428893	1	61	theme	O	106:106	arg1	antigen					108:114	The O antigen	102:114	The O antigen	102:114	The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria.
25428893	2	62	theme	antigen	254:260	arg1	structure					235:243	structure	235:243	structure	235:243	The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity.
25428893	2	62	theme	antigen	254:260	arg1	composition					219:229	chemical composition	210:229	chemical composition	210:229	The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity.
25428893	1	63	from	bacteria	196:203	arg1	part					142:145	the outermost part	128:145	the outermost part of the lipopolysaccharide layer in Gram-negative bacteria	128:203	The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria.
25428893	2	64	theme	O	252:252	arg1	antigen					254:260	the O antigen	248:260	the O antigen	248:260	The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity.
25428893	1	65	from	layer	173:177	arg1	bacteria					196:203	Gram-negative bacteria	182:203	Gram-negative bacteria	182:203	The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria.
25428893	2	66	theme	single	306:311	arg1	species					313:319	a single species	304:319	a single species revealing itself as serological diversity	304:361	The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity.
25428893	8	67	theme	typing	1291:1296	arg1	approaches					1298:1307	traditional typing approaches	1279:1307	traditional typing approaches	1279:1307	These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
25428893	0	68	theme	Escherichia	48:58	arg1	cluster					93:99	the Escherichia coli O-antigen biosynthesis gene cluster	44:99	the Escherichia coli O-antigen biosynthesis gene cluster	44:99	A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.
25428893	6	69	contain	have	889:892	arg2	background					911:920	a unique genetic background	894:920	a unique genetic background allowing a more successful exchange of O-AGCs	894:966	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	6	69	contain	have	889:892	arg1	lineage					870:876	the ST10 lineage	861:876	the ST10 lineage	861:876	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	2	70	dep	composition	219:229	arg1	The					206:208	The	206:208	The	206:208	The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity.
25428893	3	71	theme	biosynthesis	423:434	arg1	clusters					441:448	the O-antigen biosynthesis gene clusters	409:448	the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups	409:511	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	3	71	theme	biosynthesis	423:434	arg1	O-AGCs					451:456	O-AGCs	451:456	O-AGCs	451:456	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	6	72	theme	O-AGCs	961:966	arg1	exchange					949:956	a more successful exchange	931:956	a more successful exchange of O-AGCs	931:966	Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.
25428893	1	73	theme	outermost	132:140	arg1	part					142:145	the outermost part	128:145	the outermost part of the lipopolysaccharide layer in Gram-negative bacteria	128:203	The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria.
25428893	3	74	from	serogroups	502:511	arg1	clusters					441:448	the O-antigen biosynthesis gene clusters	409:448	the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups	409:511	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	3	74	from	serogroups	502:511	arg1	O-AGCs					451:456	O-AGCs	451:456	O-AGCs	451:456	Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups.
25428893	7	75	from	coli	1043:1046	arg1	view					997:1000	a complete view	986:1000	a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized	986:1170	Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.
26991030	10	0	theme	physical	1502:1509	arg1	stimulus					1511:1518	this physical stimulus	1497:1518	this physical stimulus	1497:1518	This correlation between EMF frequency and 3D-printed chondrocytes suggests the necessity for optimization of EMF parameters when this physical stimulus is applied to engineered cartilage.
26991030	10	1	theme	EMF	1392:1394	arg1	frequency					1396:1404	EMF frequency	1392:1404	EMF frequency	1392:1404	This correlation between EMF frequency and 3D-printed chondrocytes suggests the necessity for optimization of EMF parameters when this physical stimulus is applied to engineered cartilage.
26991030	4	2	theme	dense	563:567	arg1	matrix					583:588	its hydrated and dense extracellular matrix	546:588	its hydrated and dense extracellular matrix	546:588	In addition to the stimulation, a scaffold is required to satisfy the characteristics of cartilage such as its hydrated and dense extracellular matrix, and a mechanical resilience to applied loads.
26991030	0	3	theme	composite	80:88	arg1	constructs					90:99	3D cell-printed composite constructs	64:99	3D cell-printed composite constructs	64:99	Effects of electromagnetic field frequencies on chondrocytes in 3D cell-printed composite constructs.
26991030	7	4	theme	extracellular	957:969	arg1	molecules					978:986	extracellular matrix molecules	957:986	extracellular matrix molecules of chondrocytes	957:1002	We found that a frequency of 45 Hz promoted gene expression and secretion of extracellular matrix molecules of chondrocytes.
26991030	9	5	theme	EMF	1267:1269	arg1	frequencies					1271:1281	different EMF frequencies	1257:1281	different EMF frequencies	1257:1281	Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo.
26991030	0	6	from	Effects	0:6	arg1	chondrocytes					48:59	chondrocytes	48:59	chondrocytes in 3D cell-printed composite constructs	48:99	Effects of electromagnetic field frequencies on chondrocytes in 3D cell-printed composite constructs.
26991030	8	7	theme	Hz	1037:1038	arg1	frequency					1020:1028	a frequency	1018:1028	a frequency of 7.5 Hz	1018:1038	In contrast, a frequency of 7.5 Hz suppressed chondrogenic differentiation in vitro.
26991030	7	8	theme	matrix	971:976	arg1	molecules					978:986	extracellular matrix molecules	957:986	extracellular matrix molecules of chondrocytes	957:1002	We found that a frequency of 45 Hz promoted gene expression and secretion of extracellular matrix molecules of chondrocytes.
26991030	1	9	theme	tissue	115:120	arg1	engineering					122:132	cartilage tissue engineering	105:132	cartilage tissue engineering	105:132	In cartilage tissue engineering, electromagnetic field (EMF) therapy has been reported to have a modest effect on promoting cartilage regeneration.
26991030	9	10	theme	consistent	1170:1179	arg1	results					1181:1187	consistent results	1170:1187	consistent results	1170:1187	Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo.
26991030	9	11	theme	cartilage	1338:1346	arg1	formation					1348:1356	cartilage formation	1338:1356	cartilage formation in vivo	1338:1364	Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo.
26991030	10	12	theme	parameters	1481:1490	arg1	optimization					1461:1472	optimization	1461:1472	optimization of EMF parameters	1461:1490	This correlation between EMF frequency and 3D-printed chondrocytes suggests the necessity for optimization of EMF parameters when this physical stimulus is applied to engineered cartilage.
26991030	6	13	theme	negative	803:810	arg1	effects					812:818	frequency-dependent positive and negative effects	770:818	frequency-dependent positive and negative effects on chondrogenesis	770:836	Here, we observed frequency-dependent positive and negative effects on chondrogenesis using a 3D cell-printed cartilage tissue.
26991030	9	14	with	pre-treatment	1238:1250	arg1	frequencies					1271:1281	different EMF frequencies	1257:1281	different EMF frequencies	1257:1281	Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo.
26991030	4	15	theme	extracellular	569:581	arg1	matrix					583:588	its hydrated and dense extracellular matrix	546:588	its hydrated and dense extracellular matrix	546:588	In addition to the stimulation, a scaffold is required to satisfy the characteristics of cartilage such as its hydrated and dense extracellular matrix, and a mechanical resilience to applied loads.
26991030	0	16	from	chondrocytes	48:59	arg1	constructs					90:99	3D cell-printed composite constructs	64:99	3D cell-printed composite constructs	64:99	Effects of electromagnetic field frequencies on chondrocytes in 3D cell-printed composite constructs.
26991030	8	17	theme	chondrogenic	1051:1062	arg1	differentiation					1064:1078	chondrogenic differentiation	1051:1078	chondrogenic differentiation	1051:1078	In contrast, a frequency of 7.5 Hz suppressed chondrogenic differentiation in vitro.
26991030	11	18	dep	©	1556:1556	arg1	Inc.					1582:1585	Inc.	1582:1585	Inc.	1582:1585	© 2016 Wiley Periodicals, Inc.
26991030	5	19	theme	chondrocyte-laden	724:740	arg1	hydrogel					742:749	a chondrocyte-laden hydrogel	722:749	a chondrocyte-laden hydrogel	722:749	Therefore, we 3D-printed a composite construct composed of a polymeric framework and a chondrocyte-laden hydrogel.
26991030	6	20	theme	positive	790:797	arg1	effects					812:818	frequency-dependent positive and negative effects	770:818	frequency-dependent positive and negative effects on chondrogenesis	770:836	Here, we observed frequency-dependent positive and negative effects on chondrogenesis using a 3D cell-printed cartilage tissue.
26991030	6	21	theme	frequency-dependent	770:788	arg1	effects					812:818	frequency-dependent positive and negative effects	770:818	frequency-dependent positive and negative effects on chondrogenesis	770:836	Here, we observed frequency-dependent positive and negative effects on chondrogenesis using a 3D cell-printed cartilage tissue.
26991030	2	22	theme	EMF	319:321	arg1	frequencies					304:314	different frequencies	294:314	different frequencies of EMF	294:321	However, these studies were conducted using different frequencies of EMF to stimulate chondrocytes.
26991030	9	23	theme	formation	1348:1356	arg1	enhancement					1323:1333	the enhancement	1319:1333	the enhancement of cartilage formation in vivo	1319:1364	Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo.
26991030	12	24	dep	Res	1602:1604	arg1	104A					1614:1617	104A	1614:1617	104A	1614:1617	J Biomed Mater Res Part A: 104A: 1797-1804, 2016.
26991030	12	24	dep	Res	1602:1604	arg1	A					1611:1611	Part A	1606:1611	J Biomed Mater Res Part A: 104A: 1797-1804, 2016.	1587:1635	J Biomed Mater Res Part A: 104A: 1797-1804, 2016.
26991030	9	25	theme	in	1229:1230	arg1	pre-treatment					1238:1250	in vitro pre-treatment	1229:1250	in vitro pre-treatment with different EMF frequencies	1229:1281	Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo.
26991030	3	26	theme	cartilage	418:426	arg1	formation					428:436	cartilage formation	418:436	cartilage formation	418:436	Thus, it is necessary to investigate the effect of EMF frequency on cartilage formation.
26991030	0	27	theme	field	27:31	arg1	frequencies					33:43	electromagnetic field frequencies	11:43	electromagnetic field frequencies	11:43	Effects of electromagnetic field frequencies on chondrocytes in 3D cell-printed composite constructs.
26991030	10	28	theme	engineered	1534:1543	arg1	cartilage					1545:1553	engineered cartilage	1534:1553	engineered cartilage	1534:1553	This correlation between EMF frequency and 3D-printed chondrocytes suggests the necessity for optimization of EMF parameters when this physical stimulus is applied to engineered cartilage.
26991030	7	29	theme	gene	924:927	arg1	expression					929:938	gene expression	924:938	gene expression	924:938	We found that a frequency of 45 Hz promoted gene expression and secretion of extracellular matrix molecules of chondrocytes.
26991030	1	30	theme	cartilage	105:113	arg1	engineering					122:132	cartilage tissue engineering	105:132	cartilage tissue engineering	105:132	In cartilage tissue engineering, electromagnetic field (EMF) therapy has been reported to have a modest effect on promoting cartilage regeneration.
26991030	9	31	theme	EMF-treated	1108:1118	arg1	constructs					1130:1139	the EMF-treated composite constructs	1104:1139	the EMF-treated composite constructs prior to implantation	1104:1161	Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo.
26991030	0	32	theme	electromagnetic	11:25	arg1	frequencies					33:43	electromagnetic field frequencies	11:43	electromagnetic field frequencies	11:43	Effects of electromagnetic field frequencies on chondrocytes in 3D cell-printed composite constructs.
26991030	4	33	theme	hydrated	550:557	arg1	matrix					583:588	its hydrated and dense extracellular matrix	546:588	its hydrated and dense extracellular matrix	546:588	In addition to the stimulation, a scaffold is required to satisfy the characteristics of cartilage such as its hydrated and dense extracellular matrix, and a mechanical resilience to applied loads.
26991030	2	34	theme	different	294:302	arg1	frequencies					304:314	different frequencies	294:314	different frequencies of EMF	294:321	However, these studies were conducted using different frequencies of EMF to stimulate chondrocytes.
26991030	9	35	theme	composite	1120:1128	arg1	constructs					1130:1139	the EMF-treated composite constructs	1104:1139	the EMF-treated composite constructs prior to implantation	1104:1161	Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo.
26991030	3	36	theme	EMF	401:403	arg1	frequency					405:413	EMF frequency	401:413	EMF frequency	401:413	Thus, it is necessary to investigate the effect of EMF frequency on cartilage formation.
26991030	6	37	from	effects	812:818	arg1	chondrogenesis					823:836	chondrogenesis	823:836	chondrogenesis	823:836	Here, we observed frequency-dependent positive and negative effects on chondrogenesis using a 3D cell-printed cartilage tissue.
26991030	1	38	theme	modest	199:204	arg1	effect					206:211	a modest effect	197:211	a modest effect	197:211	In cartilage tissue engineering, electromagnetic field (EMF) therapy has been reported to have a modest effect on promoting cartilage regeneration.
26991030	0	39	theme	frequencies	33:43	arg1	Effects					0:6	Effects	0:6	Effects of electromagnetic field frequencies on chondrocytes in 3D cell-printed composite constructs	0:99	Effects of electromagnetic field frequencies on chondrocytes in 3D cell-printed composite constructs.
26991030	3	40	theme	frequency	405:413	arg1	effect					391:396	the effect	387:396	the effect of EMF frequency on cartilage formation	387:436	Thus, it is necessary to investigate the effect of EMF frequency on cartilage formation.
26991030	6	41	theme	cartilage	862:870	arg1	tissue					872:877	a 3D cell-printed cartilage tissue	844:877	a 3D cell-printed cartilage tissue	844:877	Here, we observed frequency-dependent positive and negative effects on chondrogenesis using a 3D cell-printed cartilage tissue.
26991030	4	42	theme	mechanical	597:606	arg1	resilience					608:617	a mechanical resilience	595:617	a mechanical resilience to applied loads	595:634	In addition to the stimulation, a scaffold is required to satisfy the characteristics of cartilage such as its hydrated and dense extracellular matrix, and a mechanical resilience to applied loads.
26991030	4	43	theme	applied	622:628	arg1	loads					630:634	applied loads	622:634	applied loads	622:634	In addition to the stimulation, a scaffold is required to satisfy the characteristics of cartilage such as its hydrated and dense extracellular matrix, and a mechanical resilience to applied loads.
26991030	6	44	theme	cell-printed	849:860	arg1	tissue					872:877	a 3D cell-printed cartilage tissue	844:877	a 3D cell-printed cartilage tissue	844:877	Here, we observed frequency-dependent positive and negative effects on chondrogenesis using a 3D cell-printed cartilage tissue.
26991030	10	45	theme	3D-printed	1410:1419	arg1	chondrocytes					1421:1432	3D-printed chondrocytes	1410:1432	3D-printed chondrocytes	1410:1432	This correlation between EMF frequency and 3D-printed chondrocytes suggests the necessity for optimization of EMF parameters when this physical stimulus is applied to engineered cartilage.
26991030	5	46	theme	polymeric	698:706	arg1	framework					708:716	a polymeric framework	696:716	a polymeric framework	696:716	Therefore, we 3D-printed a composite construct composed of a polymeric framework and a chondrocyte-laden hydrogel.
26991030	4	47	theme	cartilage	528:536	arg1	resilience					608:617	a mechanical resilience	595:617	a mechanical resilience to applied loads	595:634	In addition to the stimulation, a scaffold is required to satisfy the characteristics of cartilage such as its hydrated and dense extracellular matrix, and a mechanical resilience to applied loads.
26991030	4	47	theme	cartilage	528:536	arg1	matrix					583:588	its hydrated and dense extracellular matrix	546:588	its hydrated and dense extracellular matrix	546:588	In addition to the stimulation, a scaffold is required to satisfy the characteristics of cartilage such as its hydrated and dense extracellular matrix, and a mechanical resilience to applied loads.
26991030	4	47	theme	cartilage	528:536	arg1	characteristics					509:523	the characteristics	505:523	the characteristics of cartilage such as its hydrated and dense extracellular matrix, and a mechanical resilience to applied loads	505:634	In addition to the stimulation, a scaffold is required to satisfy the characteristics of cartilage such as its hydrated and dense extracellular matrix, and a mechanical resilience to applied loads.
26991030	6	48	theme	3D	846:847	arg1	tissue					872:877	a 3D cell-printed cartilage tissue	844:877	a 3D cell-printed cartilage tissue	844:877	Here, we observed frequency-dependent positive and negative effects on chondrogenesis using a 3D cell-printed cartilage tissue.
26991030	12	49	dep	104A	1614:1617	arg1	1797-1804					1620:1628	1797-1804	1620:1628	1797-1804	1620:1628	J Biomed Mater Res Part A: 104A: 1797-1804, 2016.
26991030	9	50	theme	different	1257:1265	arg1	frequencies					1271:1281	different EMF frequencies	1257:1281	different EMF frequencies	1257:1281	Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo.
26991030	5	51	theme	composite	664:672	arg1	construct					674:682	a composite construct	662:682	a composite construct composed of a polymeric framework and a chondrocyte-laden hydrogel	662:749	Therefore, we 3D-printed a composite construct composed of a polymeric framework and a chondrocyte-laden hydrogel.
26991030	10	52	theme	EMF	1477:1479	arg1	parameters					1481:1490	EMF parameters	1477:1490	EMF parameters	1477:1490	This correlation between EMF frequency and 3D-printed chondrocytes suggests the necessity for optimization of EMF parameters when this physical stimulus is applied to engineered cartilage.
26991030	1	53	theme	electromagnetic	135:149	arg1	EMF					158:160	EMF	158:160	EMF	158:160	In cartilage tissue engineering, electromagnetic field (EMF) therapy has been reported to have a modest effect on promoting cartilage regeneration.
26991030	1	53	theme	electromagnetic	135:149	arg1	field					151:155	electromagnetic field	135:155	electromagnetic field (EMF) therapy	135:169	In cartilage tissue engineering, electromagnetic field (EMF) therapy has been reported to have a modest effect on promoting cartilage regeneration.
26991030	7	54	theme	Hz	912:913	arg1	frequency					896:904	a frequency	894:904	a frequency of 45 Hz	894:913	We found that a frequency of 45 Hz promoted gene expression and secretion of extracellular matrix molecules of chondrocytes.
26991030	12	55	theme	Part	1606:1609	arg1	A					1611:1611	Part A	1606:1611	J Biomed Mater Res Part A: 104A: 1797-1804, 2016.	1587:1635	J Biomed Mater Res Part A: 104A: 1797-1804, 2016.
26991030	9	56	theme	different	1292:1300	arg1	capabilities					1302:1313	different capabilities	1292:1313	different capabilities	1292:1313	Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo.
26991030	7	57	theme	chondrocytes	991:1002	arg1	molecules					978:986	extracellular matrix molecules	957:986	extracellular matrix molecules of chondrocytes	957:1002	We found that a frequency of 45 Hz promoted gene expression and secretion of extracellular matrix molecules of chondrocytes.
26991030	1	58	theme	field	151:155	arg1	therapy					163:169	electromagnetic field (EMF) therapy	135:169	electromagnetic field (EMF) therapy	135:169	In cartilage tissue engineering, electromagnetic field (EMF) therapy has been reported to have a modest effect on promoting cartilage regeneration.
26991030	0	59	theme	cell-printed	67:78	arg1	constructs					90:99	3D cell-printed composite constructs	64:99	3D cell-printed composite constructs	64:99	Effects of electromagnetic field frequencies on chondrocytes in 3D cell-printed composite constructs.
26991030	1	60	contain	have	192:195	arg2	effect					206:211	a modest effect	197:211	a modest effect	197:211	In cartilage tissue engineering, electromagnetic field (EMF) therapy has been reported to have a modest effect on promoting cartilage regeneration.
26991030	1	60	contain	have	192:195	arg1	therapy					163:169	electromagnetic field (EMF) therapy	135:169	electromagnetic field (EMF) therapy	135:169	In cartilage tissue engineering, electromagnetic field (EMF) therapy has been reported to have a modest effect on promoting cartilage regeneration.
26991030	1	61	theme	cartilage	226:234	arg1	regeneration					236:247	cartilage regeneration	226:247	cartilage regeneration	226:247	In cartilage tissue engineering, electromagnetic field (EMF) therapy has been reported to have a modest effect on promoting cartilage regeneration.
26991030	0	62	theme	3D	64:65	arg1	constructs					90:99	3D cell-printed composite constructs	64:99	3D cell-printed composite constructs	64:99	Effects of electromagnetic field frequencies on chondrocytes in 3D cell-printed composite constructs.
26991030	3	63	from	effect	391:396	arg1	formation					428:436	cartilage formation	418:436	cartilage formation	418:436	Thus, it is necessary to investigate the effect of EMF frequency on cartilage formation.
26991030	7	64	theme	molecules	978:986	arg1	expression					929:938	gene expression	924:938	gene expression	924:938	We found that a frequency of 45 Hz promoted gene expression and secretion of extracellular matrix molecules of chondrocytes.
26991030	7	64	theme	molecules	978:986	arg1	secretion					944:952	secretion	944:952	secretion	944:952	We found that a frequency of 45 Hz promoted gene expression and secretion of extracellular matrix molecules of chondrocytes.
26991030	9	65	dep	in	1229:1230	arg1	vitro					1232:1236	vitro	1232:1236	vitro	1232:1236	Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo.
26118827	0	0	theme	alginate	90:97	arg1	microspheres					99:110	bFGF-loaded alginate microspheres	78:110	bFGF-loaded alginate microspheres	78:110	Acceleration of skin regeneration in full-thickness burns by incorporation of bFGF-loaded alginate microspheres into a CMCS-PVA hydrogel.
26118827	6	1	theme	bFGF	790:793	arg1	release					779:785	the release	775:785	the release of bFGF from Ms-CMCS-PVA in a sustained manner	775:832	In an in vitro study, the release of bFGF from Ms-CMCS-PVA in a sustained manner retained higher bioactivity over a 2-week period.
26118827	1	2	theme	skin	162:165	arg1	engineering					174:184	skin tissue engineering	162:184	skin tissue engineering	162:184	Two important issues in skin tissue engineering are the vascularization and regeneration of the dermis.
26118827	9	3	theme	mature	1330:1335	arg1	vessels					1343:1349	newly formed and mature blood vessels	1313:1349	newly formed and mature blood vessels	1313:1349	Moreover, the bFGF/Ms-CMCS-PVA group had the highest density of newly formed and mature blood vessels during the 2 mweek treatment period.
26118827	0	4	theme	bFGF-loaded	78:88	arg1	microspheres					99:110	bFGF-loaded alginate microspheres	78:110	bFGF-loaded alginate microspheres	78:110	Acceleration of skin regeneration in full-thickness burns by incorporation of bFGF-loaded alginate microspheres into a CMCS-PVA hydrogel.
26118827	6	5	theme	sustained	817:825	arg1	manner					827:832	a sustained manner	815:832	a sustained manner	815:832	In an in vitro study, the release of bFGF from Ms-CMCS-PVA in a sustained manner retained higher bioactivity over a 2-week period.
26118827	10	6	from	potential	1505:1513	arg1	regeneration					1540:1551	dermal tissue regeneration	1526:1551	dermal tissue regeneration	1526:1551	The ability of the bFGF/Ms-CMCS-PVA hydrogel to accelerate wound healing in a full-thickness burn model suggests its potential for use in dermal tissue regeneration.
26118827	1	7	theme	tissue	167:172	arg1	engineering					174:184	skin tissue engineering	162:184	skin tissue engineering	162:184	Two important issues in skin tissue engineering are the vascularization and regeneration of the dermis.
26118827	4	8	theme	CMCS	546:549	arg1	-poly					551:555	carboxymethyl chitosan (CMCS)-poly	522:555	carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA)	522:576	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	4	8	theme	CMCS	546:549	arg1	alcohol					563:569	vinyl alcohol	557:569	vinyl alcohol	557:569	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	4	8	theme	CMCS	546:549	arg1	PVA					573:575	PVA	573:575	PVA	573:575	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	3	9	theme	bFGF	362:365	arg1	delivery					350:357	Direct delivery	343:357	Direct delivery of bFGF to the wound area	343:383	Direct delivery of bFGF to the wound area, however, would lead to a loss of bioactivity.
26118827	0	10	from	Acceleration	0:11	arg1	burns					52:56	full-thickness burns	37:56	full-thickness burns	37:56	Acceleration of skin regeneration in full-thickness burns by incorporation of bFGF-loaded alginate microspheres into a CMCS-PVA hydrogel.
26118827	7	11	theme	Full-thickness	884:897	arg1	wounds					904:909	Full-thickness burn wounds	884:909	Full-thickness burn wounds	884:909	Full-thickness burn wounds were created in the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel, with and without bFGF.
26118827	9	12	contain	had	1286:1288	arg2	density					1302:1308	the highest density	1290:1308	the highest density of newly formed and mature blood vessels	1290:1349	Moreover, the bFGF/Ms-CMCS-PVA group had the highest density of newly formed and mature blood vessels during the 2 mweek treatment period.
26118827	9	12	contain	had	1286:1288	arg1	group					1280:1284	the bFGF/Ms-CMCS-PVA group	1259:1284	the bFGF/Ms-CMCS-PVA group	1259:1284	Moreover, the bFGF/Ms-CMCS-PVA group had the highest density of newly formed and mature blood vessels during the 2 mweek treatment period.
26118827	9	13	theme	vessels	1343:1349	arg1	density					1302:1308	the highest density	1290:1308	the highest density of newly formed and mature blood vessels	1290:1349	Moreover, the bFGF/Ms-CMCS-PVA group had the highest density of newly formed and mature blood vessels during the 2 mweek treatment period.
26118827	0	14	theme	microspheres	99:110	arg1	incorporation					61:73	incorporation	61:73	incorporation of bFGF-loaded alginate microspheres into a CMCS-PVA hydrogel	61:135	Acceleration of skin regeneration in full-thickness burns by incorporation of bFGF-loaded alginate microspheres into a CMCS-PVA hydrogel.
26118827	1	15	from	issues	152:157	arg1	engineering					174:184	skin tissue engineering	162:184	skin tissue engineering	162:184	Two important issues in skin tissue engineering are the vascularization and regeneration of the dermis.
26118827	10	16	theme	bFGF/Ms-CMCS-PVA	1407:1422	arg1	hydrogel					1424:1431	the bFGF/Ms-CMCS-PVA hydrogel	1403:1431	the bFGF/Ms-CMCS-PVA hydrogel	1403:1431	The ability of the bFGF/Ms-CMCS-PVA hydrogel to accelerate wound healing in a full-thickness burn model suggests its potential for use in dermal tissue regeneration.
26118827	1	17	theme	important	142:150	arg1	vascularization					194:208	vascularization	194:208	vascularization	194:208	Two important issues in skin tissue engineering are the vascularization and regeneration of the dermis.
26118827	1	17	theme	important	142:150	arg1	issues					152:157	Two important issues	138:157	Two important issues in skin tissue engineering	138:184	Two important issues in skin tissue engineering are the vascularization and regeneration of the dermis.
26118827	0	18	theme	CMCS-PVA	119:126	arg1	hydrogel					128:135	a CMCS-PVA hydrogel	117:135	a CMCS-PVA hydrogel	117:135	Acceleration of skin regeneration in full-thickness burns by incorporation of bFGF-loaded alginate microspheres into a CMCS-PVA hydrogel.
26118827	8	19	theme	bFGF-CMCS-PVA	1090:1102	arg1	groups					1104:1109	the control, CMCS-PVA and bFGF-CMCS-PVA groups	1064:1109	groups	1104:1109	Compared with the control, CMCS-PVA and bFGF-CMCS-PVA groups, the bFGF/Ms-CMCS-PVA group revealed significantly faster wound recovery rates, with re-epithelialization and regeneration of the dermis.
26118827	4	20	theme	composite	588:596	arg1	hydrogel					598:605	a composite hydrogel	586:605	a composite hydrogel	586:605	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	6	21	from	Ms-CMCS-PVA	800:810	arg1	release					779:785	the release	775:785	the release of bFGF from Ms-CMCS-PVA in a sustained manner	775:832	In an in vitro study, the release of bFGF from Ms-CMCS-PVA in a sustained manner retained higher bioactivity over a 2-week period.
26118827	4	22	theme	alginate	457:464	arg1	Ms					480:481	Ms	480:481	Ms	480:481	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	4	22	theme	alginate	457:464	arg1	microspheres					466:477	bFGF-loaded alginate microspheres	445:477	bFGF-loaded alginate microspheres (Ms)	445:482	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	7	23	theme	CMCS-PVA	1008:1015	arg1	hydrogel					1017:1024	CMCS-PVA hydrogel	1008:1024	CMCS-PVA hydrogel	1008:1024	Full-thickness burn wounds were created in the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel, with and without bFGF.
26118827	8	24	theme	CMCS-PVA	1077:1084	arg1	groups					1104:1109	the control, CMCS-PVA and bFGF-CMCS-PVA groups	1064:1109	groups	1104:1109	Compared with the control, CMCS-PVA and bFGF-CMCS-PVA groups, the bFGF/Ms-CMCS-PVA group revealed significantly faster wound recovery rates, with re-epithelialization and regeneration of the dermis.
26118827	10	25	theme	burn	1481:1484	arg1	model					1486:1490	a full-thickness burn model	1464:1490	a full-thickness burn model	1464:1490	The ability of the bFGF/Ms-CMCS-PVA hydrogel to accelerate wound healing in a full-thickness burn model suggests its potential for use in dermal tissue regeneration.
26118827	10	26	theme	dermal	1526:1531	arg1	regeneration					1540:1551	dermal tissue regeneration	1526:1551	dermal tissue regeneration	1526:1551	The ability of the bFGF/Ms-CMCS-PVA hydrogel to accelerate wound healing in a full-thickness burn model suggests its potential for use in dermal tissue regeneration.
26118827	4	27	theme	bFGF-loaded	445:455	arg1	Ms					480:481	Ms	480:481	Ms	480:481	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	4	27	theme	bFGF-loaded	445:455	arg1	microspheres					466:477	bFGF-loaded alginate microspheres	445:477	bFGF-loaded alginate microspheres (Ms)	445:482	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	7	28	theme	regeneration	982:993	arg1	evaluation					963:972	in vivo evaluation	955:972	in vivo evaluation of skin regeneration	955:993	Full-thickness burn wounds were created in the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel, with and without bFGF.
26118827	6	29	dep	in	759:760	arg1	vitro					762:766	vitro	762:766	vitro	762:766	In an in vitro study, the release of bFGF from Ms-CMCS-PVA in a sustained manner retained higher bioactivity over a 2-week period.
26118827	7	30	theme	in	955:956	arg1	evaluation					963:972	in vivo evaluation	955:972	in vivo evaluation of skin regeneration	955:993	Full-thickness burn wounds were created in the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel, with and without bFGF.
26118827	8	31	theme	wound	1169:1173	arg1	rates					1184:1188	significantly faster wound recovery rates	1148:1188	significantly faster wound recovery rates	1148:1188	Compared with the control, CMCS-PVA and bFGF-CMCS-PVA groups, the bFGF/Ms-CMCS-PVA group revealed significantly faster wound recovery rates, with re-epithelialization and regeneration of the dermis.
26118827	5	32	theme	CMCS-PVA	743:750	arg1	structure					730:738	the inner structure	720:738	the inner structure of CMCS-PVA	720:750	Scanning electron microscopy (SEM) results indicated that the incorporation of Ms does not significantly affect the inner structure of CMCS-PVA.
26118827	4	33	theme	carboxymethyl	522:534	arg1	-poly					551:555	carboxymethyl chitosan (CMCS)-poly	522:555	carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA)	522:576	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	4	33	theme	carboxymethyl	522:534	arg1	alcohol					563:569	vinyl alcohol	557:569	vinyl alcohol	557:569	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	4	33	theme	carboxymethyl	522:534	arg1	PVA					573:575	PVA	573:575	PVA	573:575	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	6	34	theme	2-week	869:874	arg1	period					876:881	a 2-week period	867:881	a 2-week period	867:881	In an in vitro study, the release of bFGF from Ms-CMCS-PVA in a sustained manner retained higher bioactivity over a 2-week period.
26118827	8	35	theme	faster	1162:1167	arg1	rates					1184:1188	significantly faster wound recovery rates	1148:1188	significantly faster wound recovery rates	1148:1188	Compared with the control, CMCS-PVA and bFGF-CMCS-PVA groups, the bFGF/Ms-CMCS-PVA group revealed significantly faster wound recovery rates, with re-epithelialization and regeneration of the dermis.
26118827	0	36	theme	regeneration	21:32	arg1	Acceleration					0:11	Acceleration	0:11	Acceleration of skin regeneration in full-thickness burns by incorporation of bFGF-loaded alginate microspheres into a CMCS-PVA hydrogel.	0:136	Acceleration of skin regeneration in full-thickness burns by incorporation of bFGF-loaded alginate microspheres into a CMCS-PVA hydrogel.
26118827	8	37	theme	control	1068:1074	arg1	groups					1104:1109	the control, CMCS-PVA and bFGF-CMCS-PVA groups	1064:1109	groups	1104:1109	Compared with the control, CMCS-PVA and bFGF-CMCS-PVA groups, the bFGF/Ms-CMCS-PVA group revealed significantly faster wound recovery rates, with re-epithelialization and regeneration of the dermis.
26118827	10	38	theme	hydrogel	1424:1431	arg1	ability					1392:1398	The ability	1388:1398	The ability of the bFGF/Ms-CMCS-PVA hydrogel to accelerate wound healing in a full-thickness burn model	1388:1490	The ability of the bFGF/Ms-CMCS-PVA hydrogel to accelerate wound healing in a full-thickness burn model suggests its potential for use in dermal tissue regeneration.
26118827	3	39	theme	wound	374:378	arg1	area					380:383	the wound area	370:383	the wound area	370:383	Direct delivery of bFGF to the wound area, however, would lead to a loss of bioactivity.
26118827	9	40	theme	bFGF/Ms-CMCS-PVA	1263:1278	arg1	group					1280:1284	the bFGF/Ms-CMCS-PVA group	1259:1284	the bFGF/Ms-CMCS-PVA group	1259:1284	Moreover, the bFGF/Ms-CMCS-PVA group had the highest density of newly formed and mature blood vessels during the 2 mweek treatment period.
26118827	7	41	theme	skin	977:980	arg1	regeneration					982:993	skin regeneration	977:993	skin regeneration	977:993	Full-thickness burn wounds were created in the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel, with and without bFGF.
26118827	8	42	theme	recovery	1175:1182	arg1	rates					1184:1188	significantly faster wound recovery rates	1148:1188	significantly faster wound recovery rates	1148:1188	Compared with the control, CMCS-PVA and bFGF-CMCS-PVA groups, the bFGF/Ms-CMCS-PVA group revealed significantly faster wound recovery rates, with re-epithelialization and regeneration of the dermis.
26118827	0	43	theme	full-thickness	37:50	arg1	burns					52:56	full-thickness burns	37:56	full-thickness burns	37:56	Acceleration of skin regeneration in full-thickness burns by incorporation of bFGF-loaded alginate microspheres into a CMCS-PVA hydrogel.
26118827	4	44	theme	chitosan	536:543	arg1	-poly					551:555	carboxymethyl chitosan (CMCS)-poly	522:555	carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA)	522:576	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	4	44	theme	chitosan	536:543	arg1	alcohol					563:569	vinyl alcohol	557:569	vinyl alcohol	557:569	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	4	44	theme	chitosan	536:543	arg1	PVA					573:575	PVA	573:575	PVA	573:575	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	2	45	theme	fibroblast	248:257	arg1	factor					266:271	Basic fibroblast growth factor	242:271	Basic fibroblast growth factor (bFGF)	242:278	Basic fibroblast growth factor (bFGF) is known to promote angiogenesis and accelerate wound healing.
26118827	2	45	theme	fibroblast	248:257	arg1	bFGF					274:277	bFGF	274:277	bFGF	274:277	Basic fibroblast growth factor (bFGF) is known to promote angiogenesis and accelerate wound healing.
26118827	9	46	theme	treatment	1370:1378	arg1	period					1380:1385	the 2 mweek treatment period	1358:1385	the 2 mweek treatment period	1358:1385	Moreover, the bFGF/Ms-CMCS-PVA group had the highest density of newly formed and mature blood vessels during the 2 mweek treatment period.
26118827	3	47	theme	bioactivity	419:429	arg1	loss					411:414	a loss	409:414	a loss of bioactivity	409:429	Direct delivery of bFGF to the wound area, however, would lead to a loss of bioactivity.
26118827	0	48	theme	skin	16:19	arg1	regeneration					21:32	skin regeneration	16:32	skin regeneration	16:32	Acceleration of skin regeneration in full-thickness burns by incorporation of bFGF-loaded alginate microspheres into a CMCS-PVA hydrogel.
26118827	11	49	dep	&	1582:1582	arg1	Sons					1584:1587	Sons	1584:1587	Sons	1584:1587	Copyright © 2015 John Wiley & Sons, Ltd.
26118827	9	50	theme	blood	1337:1341	arg1	vessels					1343:1349	newly formed and mature blood vessels	1313:1349	newly formed and mature blood vessels	1313:1349	Moreover, the bFGF/Ms-CMCS-PVA group had the highest density of newly formed and mature blood vessels during the 2 mweek treatment period.
26118827	1	51	dep	vascularization	194:208	arg1	the					190:192	the	190:192	the	190:192	Two important issues in skin tissue engineering are the vascularization and regeneration of the dermis.
26118827	7	52	theme	dorsal	931:936	arg1	area					938:941	the dorsal area	927:941	the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel	927:1024	Full-thickness burn wounds were created in the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel, with and without bFGF.
26118827	7	53	dep	in	955:956	arg1	vivo					958:961	vivo	958:961	vivo	958:961	Full-thickness burn wounds were created in the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel, with and without bFGF.
26118827	5	54	theme	inner	724:728	arg1	structure					730:738	the inner structure	720:738	the inner structure of CMCS-PVA	720:750	Scanning electron microscopy (SEM) results indicated that the incorporation of Ms does not significantly affect the inner structure of CMCS-PVA.
26118827	5	55	theme	Scanning	608:615	arg1	microscopy					626:635	Scanning electron microscopy	608:635	Scanning electron microscopy (SEM) results	608:649	Scanning electron microscopy (SEM) results indicated that the incorporation of Ms does not significantly affect the inner structure of CMCS-PVA.
26118827	5	55	theme	Scanning	608:615	arg1	SEM					638:640	SEM	638:640	SEM	638:640	Scanning electron microscopy (SEM) results indicated that the incorporation of Ms does not significantly affect the inner structure of CMCS-PVA.
26118827	10	56	theme	full-thickness	1466:1479	arg1	model					1486:1490	a full-thickness burn model	1464:1490	a full-thickness burn model	1464:1490	The ability of the bFGF/Ms-CMCS-PVA hydrogel to accelerate wound healing in a full-thickness burn model suggests its potential for use in dermal tissue regeneration.
26118827	10	57	theme	wound	1447:1451	arg1	healing					1453:1459	wound healing	1447:1459	wound healing	1447:1459	The ability of the bFGF/Ms-CMCS-PVA hydrogel to accelerate wound healing in a full-thickness burn model suggests its potential for use in dermal tissue regeneration.
26118827	8	58	theme	bFGF/Ms-CMCS-PVA	1116:1131	arg1	group					1133:1137	the bFGF/Ms-CMCS-PVA group	1112:1137	the bFGF/Ms-CMCS-PVA group	1112:1137	Compared with the control, CMCS-PVA and bFGF-CMCS-PVA groups, the bFGF/Ms-CMCS-PVA group revealed significantly faster wound recovery rates, with re-epithelialization and regeneration of the dermis.
26118827	9	59	theme	highest	1294:1300	arg1	density					1302:1308	the highest density	1290:1308	the highest density of newly formed and mature blood vessels	1290:1349	Moreover, the bFGF/Ms-CMCS-PVA group had the highest density of newly formed and mature blood vessels during the 2 mweek treatment period.
26118827	5	60	theme	electron	617:624	arg1	microscopy					626:635	Scanning electron microscopy	608:635	Scanning electron microscopy (SEM) results	608:649	Scanning electron microscopy (SEM) results indicated that the incorporation of Ms does not significantly affect the inner structure of CMCS-PVA.
26118827	5	60	theme	electron	617:624	arg1	SEM					638:640	SEM	638:640	SEM	638:640	Scanning electron microscopy (SEM) results indicated that the incorporation of Ms does not significantly affect the inner structure of CMCS-PVA.
26118827	6	61	theme	higher	843:848	arg1	bioactivity					850:860	higher bioactivity	843:860	higher bioactivity	843:860	In an in vitro study, the release of bFGF from Ms-CMCS-PVA in a sustained manner retained higher bioactivity over a 2-week period.
26118827	5	62	theme	microscopy	626:635	arg1	results					643:649	Scanning electron microscopy (SEM) results	608:649	Scanning electron microscopy (SEM) results	608:649	Scanning electron microscopy (SEM) results indicated that the incorporation of Ms does not significantly affect the inner structure of CMCS-PVA.
26118827	2	63	theme	Basic	242:246	arg1	factor					266:271	Basic fibroblast growth factor	242:271	Basic fibroblast growth factor (bFGF)	242:278	Basic fibroblast growth factor (bFGF) is known to promote angiogenesis and accelerate wound healing.
26118827	2	63	theme	Basic	242:246	arg1	bFGF					274:277	bFGF	274:277	bFGF	274:277	Basic fibroblast growth factor (bFGF) is known to promote angiogenesis and accelerate wound healing.
26118827	6	64	from	release	779:785	arg1	manner					827:832	a sustained manner	815:832	a sustained manner	815:832	In an in vitro study, the release of bFGF from Ms-CMCS-PVA in a sustained manner retained higher bioactivity over a 2-week period.
26118827	1	65	theme	dermis	234:239	arg1	regeneration					214:225	regeneration	214:225	regeneration	214:225	Two important issues in skin tissue engineering are the vascularization and regeneration of the dermis.
26118827	1	65	theme	dermis	234:239	arg1	vascularization					194:208	vascularization	194:208	vascularization	194:208	Two important issues in skin tissue engineering are the vascularization and regeneration of the dermis.
26118827	1	65	theme	dermis	234:239	arg1	issues					152:157	Two important issues	138:157	Two important issues in skin tissue engineering	138:184	Two important issues in skin tissue engineering are the vascularization and regeneration of the dermis.
26118827	7	66	theme	rats	946:949	arg1	area					938:941	the dorsal area	927:941	the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel	927:1024	Full-thickness burn wounds were created in the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel, with and without bFGF.
26118827	6	67	theme	in	759:760	arg1	study					768:772	an in vitro study	756:772	an in vitro study	756:772	In an in vitro study, the release of bFGF from Ms-CMCS-PVA in a sustained manner retained higher bioactivity over a 2-week period.
26118827	2	68	theme	wound	328:332	arg1	healing					334:340	wound healing	328:340	wound healing	328:340	Basic fibroblast growth factor (bFGF) is known to promote angiogenesis and accelerate wound healing.
26118827	4	69	theme	vinyl	557:561	arg1	-poly					551:555	carboxymethyl chitosan (CMCS)-poly	522:555	carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA)	522:576	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	4	69	theme	vinyl	557:561	arg1	alcohol					563:569	vinyl alcohol	557:569	vinyl alcohol	557:569	To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel.
26118827	5	70	theme	Ms	687:688	arg1	incorporation					670:682	the incorporation	666:682	the incorporation of Ms	666:688	Scanning electron microscopy (SEM) results indicated that the incorporation of Ms does not significantly affect the inner structure of CMCS-PVA.
26118827	10	71	theme	tissue	1533:1538	arg1	regeneration					1540:1551	dermal tissue regeneration	1526:1551	dermal tissue regeneration	1526:1551	The ability of the bFGF/Ms-CMCS-PVA hydrogel to accelerate wound healing in a full-thickness burn model suggests its potential for use in dermal tissue regeneration.
26118827	8	72	theme	dermis	1241:1246	arg1	regeneration					1221:1232	regeneration	1221:1232	regeneration	1221:1232	Compared with the control, CMCS-PVA and bFGF-CMCS-PVA groups, the bFGF/Ms-CMCS-PVA group revealed significantly faster wound recovery rates, with re-epithelialization and regeneration of the dermis.
26118827	8	72	theme	dermis	1241:1246	arg1	re-epithelialization					1196:1215	re-epithelialization	1196:1215	re-epithelialization	1196:1215	Compared with the control, CMCS-PVA and bFGF-CMCS-PVA groups, the bFGF/Ms-CMCS-PVA group revealed significantly faster wound recovery rates, with re-epithelialization and regeneration of the dermis.
26118827	9	73	theme	formed	1319:1324	arg1	vessels					1343:1349	newly formed and mature blood vessels	1313:1349	newly formed and mature blood vessels	1313:1349	Moreover, the bFGF/Ms-CMCS-PVA group had the highest density of newly formed and mature blood vessels during the 2 mweek treatment period.
26118827	3	74	theme	Direct	343:348	arg1	delivery					350:357	Direct delivery	343:357	Direct delivery of bFGF to the wound area	343:383	Direct delivery of bFGF to the wound area, however, would lead to a loss of bioactivity.
26118827	9	75	theme	mweek	1364:1368	arg1	period					1380:1385	the 2 mweek treatment period	1358:1385	the 2 mweek treatment period	1358:1385	Moreover, the bFGF/Ms-CMCS-PVA group had the highest density of newly formed and mature blood vessels during the 2 mweek treatment period.
26118827	2	76	theme	growth	259:264	arg1	factor					266:271	Basic fibroblast growth factor	242:271	Basic fibroblast growth factor (bFGF)	242:278	Basic fibroblast growth factor (bFGF) is known to promote angiogenesis and accelerate wound healing.
26118827	2	76	theme	growth	259:264	arg1	bFGF					274:277	bFGF	274:277	bFGF	274:277	Basic fibroblast growth factor (bFGF) is known to promote angiogenesis and accelerate wound healing.
26118827	7	77	theme	burn	899:902	arg1	wounds					904:909	Full-thickness burn wounds	884:909	Full-thickness burn wounds	884:909	Full-thickness burn wounds were created in the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel, with and without bFGF.
25579910	0	0	theme	Lithospermum	85:96	arg1	microcapsules					112:124	Lithospermum erythrorhizon microcapsules	85:124	Lithospermum erythrorhizon microcapsules	85:124	The optimal extracting process, manufacturing technique and biological evaluation of Lithospermum erythrorhizon microcapsules.
25579910	7	1	theme	L.	1239:1240	arg1	SPL					1271:1273	SPL	1271:1273	SPL	1271:1273	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	7	1	theme	L.	1239:1240	arg1	microcapsules					1256:1268	the L. erythrorhizon microcapsules	1235:1268	the L. erythrorhizon microcapsules (SPL)	1235:1274	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	3	2	theme	antibacterial	544:556	arg1	property					558:565	then the antibacterial property	535:565	then the antibacterial property	535:565	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	5	3	theme	animal	990:995	arg1	study					997:1001	animal study	990:1001	animal study	990:1001	The cell viability test and animal study are performed on L. erythrorhizon microcapsules.
25579910	3	4	theme	erythrorhizon	596:608	arg1	extract					610:616	L. erythrorhizon extract	593:616	L. erythrorhizon extract	593:616	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	5	5	dep	viability	971:979	arg1	test					981:984	test	981:984	test	981:984	The cell viability test and animal study are performed on L. erythrorhizon microcapsules.
25579910	5	5	dep	viability	971:979	arg1	study					997:1001	animal study	990:1001	animal study	990:1001	The cell viability test and animal study are performed on L. erythrorhizon microcapsules.
25579910	0	6	theme	microcapsules	112:124	arg1	technique					46:54	technique	46:54	technique	46:54	The optimal extracting process, manufacturing technique and biological evaluation of Lithospermum erythrorhizon microcapsules.
25579910	0	6	theme	microcapsules	112:124	arg1	evaluation					71:80	biological evaluation	60:80	biological evaluation of Lithospermum erythrorhizon microcapsules	60:124	The optimal extracting process, manufacturing technique and biological evaluation of Lithospermum erythrorhizon microcapsules.
25579910	3	7	theme	various	637:643	arg1	concentrations					645:658	various concentrations	637:658	various concentrations of L. erythrorhizon extract solution	637:695	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	3	8	theme	erythrorhizon	512:524	arg1	extract					526:532	L. erythrorhizon extract	509:532	L. erythrorhizon extract	509:532	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	6	9	theme	experiment	1056:1065	arg1	results					1067:1073	The experiment results	1052:1073	The experiment results	1052:1073	The experiment results show that sodium alginate/pectin L. erythrorhizon (SPL) microcapsules possess a 120-hour drug release.
25579910	0	10	theme	erythrorhizon	98:110	arg1	microcapsules					112:124	Lithospermum erythrorhizon microcapsules	85:124	Lithospermum erythrorhizon microcapsules	85:124	The optimal extracting process, manufacturing technique and biological evaluation of Lithospermum erythrorhizon microcapsules.
25579910	3	11	theme	extract	680:686	arg1	solution					688:695	L. erythrorhizon extract solution	663:695	L. erythrorhizon extract solution	663:695	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	2	12	from	temperatures	333:344	arg1	ethanol					256:262	ethanol	256:262	ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order	256:414	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	6	13	theme	L.	1108:1109	arg1	SPL					1126:1128	SPL	1126:1128	SPL	1126:1128	The experiment results show that sodium alginate/pectin L. erythrorhizon (SPL) microcapsules possess a 120-hour drug release.
25579910	6	13	theme	L.	1108:1109	arg1	erythrorhizon					1111:1123	sodium alginate/pectin L. erythrorhizon	1085:1123	sodium alginate/pectin L. erythrorhizon (SPL)	1085:1129	The experiment results show that sodium alginate/pectin L. erythrorhizon (SPL) microcapsules possess a 120-hour drug release.
25579910	3	14	theme	solution	688:695	arg1	concentrations					645:658	various concentrations	637:658	various concentrations of L. erythrorhizon extract solution	637:695	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	3	14	theme	solution	688:695	arg1	weights					711:717	different weights	701:717	different weights of L. erythrorhizon extract powder	701:752	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	3	15	theme	optimal	457:463	arg1	parameters					465:474	The optimal parameters	453:474	The optimal parameters	453:474	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	7	16	theme	erythrorhizon	1242:1254	arg1	SPL					1271:1273	SPL	1271:1273	SPL	1271:1273	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	7	16	theme	erythrorhizon	1242:1254	arg1	microcapsules					1256:1268	the L. erythrorhizon microcapsules	1235:1268	the L. erythrorhizon microcapsules (SPL)	1235:1274	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	2	17	theme	optimal	433:439	arg1	parameters					441:450	the optimal parameters	429:450	the optimal parameters	429:450	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	3	18	theme	L.	663:664	arg1	solution					688:695	L. erythrorhizon extract solution	663:695	L. erythrorhizon extract solution	663:695	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	6	19	theme	sodium	1085:1090	arg1	SPL					1126:1128	SPL	1126:1128	SPL	1126:1128	The experiment results show that sodium alginate/pectin L. erythrorhizon (SPL) microcapsules possess a 120-hour drug release.
25579910	6	19	theme	sodium	1085:1090	arg1	erythrorhizon					1111:1123	sodium alginate/pectin L. erythrorhizon	1085:1123	sodium alginate/pectin L. erythrorhizon (SPL)	1085:1129	The experiment results show that sodium alginate/pectin L. erythrorhizon (SPL) microcapsules possess a 120-hour drug release.
25579910	7	20	theme	healing	1333:1339	arg1	process					1341:1347	the wound healing process	1323:1347	the wound healing process	1323:1347	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	1	21	theme	recent	198:203	arg1	studies					205:211	recent studies	198:211	recent studies	198:211	Lithospermum erythrorhizon has been proved to be anti-inflammatory, by recent studies.
25579910	0	22	theme	optimal	4:10	arg1	process					23:29	The optimal extracting process,	0:30	process	23:29	The optimal extracting process, manufacturing technique and biological evaluation of Lithospermum erythrorhizon microcapsules.
25579910	3	23	theme	erythrorhizon	666:678	arg1	solution					688:695	L. erythrorhizon extract solution	663:695	L. erythrorhizon extract solution	663:695	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	7	24	theme	size	1360:1363	arg1	reduction					1365:1373	the wound size reduction	1350:1373	the wound size reduction	1350:1373	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	4	25	theme	different	889:897	arg1	weights					899:905	different weights	889:905	different weights of powder	889:915	The concentrations of solution are 0.1mg/ml, 0.5mg/ml, 1.0mg/ml, and 2.0mg/ml and ethanol is chosen as the solvent, and different weights of powder are varied as 0.1mg, 1.0mg, 2.0mg, and 10mg.
25579910	3	26	theme	L.	509:510	arg1	extract					526:532	L. erythrorhizon extract	509:532	L. erythrorhizon extract	509:532	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	2	27	dep	ratios	288:293	arg1	1:6					301:303	1:6	301:303	1:6	301:303	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	27	dep	ratios	288:293	arg1	1:4					296:298	1:4	296:298	1:4	296:298	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	27	dep	ratios	288:293	arg1	1:8					306:308	1:8	306:308	1:8	306:308	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	27	dep	ratios	288:293	arg1	1:12					315:318	1:12	315:318	1:12	315:318	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	7	28	theme	good	1281:1284	arg1	viability					1291:1299	good cell viability	1281:1299	good cell viability (99%)	1281:1305	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	7	28	theme	good	1281:1284	arg1	%					1304:1304	99%	1302:1304	99%	1302:1304	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	7	29	theme	cell	1286:1289	arg1	viability					1291:1299	good cell viability	1281:1299	good cell viability (99%)	1281:1305	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	7	29	theme	cell	1286:1289	arg1	%					1304:1304	99%	1302:1304	99%	1302:1304	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	2	30	dep	temperatures	333:344	arg1	60°C					363:366	60°C	363:366	60°C	363:366	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	30	dep	temperatures	333:344	arg1	40°C					347:350	40°C	347:350	40°C	347:350	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	30	dep	temperatures	333:344	arg1	temperatures					333:344	extraction temperatures	322:344	extraction temperatures (40°C, 50°C, and 60°C)	322:367	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	30	dep	temperatures	333:344	arg1	50°C					353:356	50°C	353:356	50°C	353:356	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	0	31	theme	extracting	12:21	arg1	process					23:29	The optimal extracting process,	0:30	process	23:29	The optimal extracting process, manufacturing technique and biological evaluation of Lithospermum erythrorhizon microcapsules.
25579910	6	32	theme	alginate/pectin	1092:1106	arg1	SPL					1126:1128	SPL	1126:1128	SPL	1126:1128	The experiment results show that sodium alginate/pectin L. erythrorhizon (SPL) microcapsules possess a 120-hour drug release.
25579910	6	32	theme	alginate/pectin	1092:1106	arg1	erythrorhizon					1111:1123	sodium alginate/pectin L. erythrorhizon	1085:1123	sodium alginate/pectin L. erythrorhizon (SPL)	1085:1129	The experiment results show that sodium alginate/pectin L. erythrorhizon (SPL) microcapsules possess a 120-hour drug release.
25579910	1	33	theme	Lithospermum	127:138	arg1	erythrorhizon					140:152	Lithospermum erythrorhizon	127:152	Lithospermum erythrorhizon	127:152	Lithospermum erythrorhizon has been proved to be anti-inflammatory, by recent studies.
25579910	2	34	theme	extraction	374:383	arg1	times					385:389	extraction times	374:389	extraction times (4, 24 and 36h)	374:405	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	7	35	from	%	1387:1387	arg1	Day					1392:1394	Day 11	1392:1397	Day 11	1392:1397	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	3	36	theme	different	701:709	arg1	weights					711:717	different weights	701:717	different weights of L. erythrorhizon extract powder	701:752	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	3	37	theme	L.	593:594	arg1	extract					610:616	L. erythrorhizon extract	593:616	L. erythrorhizon extract	593:616	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	6	38	theme	drug	1164:1167	arg1	release					1169:1175	a 120-hour drug release	1153:1175	a 120-hour drug release	1153:1175	The experiment results show that sodium alginate/pectin L. erythrorhizon (SPL) microcapsules possess a 120-hour drug release.
25579910	2	39	from	ratios	288:293	arg1	ethanol					256:262	ethanol	256:262	ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order	256:414	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	7	40	theme	animal	1212:1217	arg1	study					1219:1223	animal study	1212:1223	animal study	1212:1223	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	3	41	theme	extract	739:745	arg1	powder					747:752	L. erythrorhizon extract powder	722:752	L. erythrorhizon extract powder	722:752	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	3	42	theme	cell	571:574	arg1	compatibility					576:588	cell compatibility	571:588	cell compatibility of L. erythrorhizon extract	571:616	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	6	43	theme	120-hour	1155:1162	arg1	release					1169:1175	a 120-hour drug release	1153:1175	a 120-hour drug release	1153:1175	The experiment results show that sodium alginate/pectin L. erythrorhizon (SPL) microcapsules possess a 120-hour drug release.
25579910	7	44	theme	study	1219:1223	arg1	results					1182:1188	The results	1178:1188	The results of cell viability and animal study	1178:1223	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	3	45	theme	powder	747:752	arg1	concentrations					645:658	various concentrations	637:658	various concentrations of L. erythrorhizon extract solution	637:695	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	3	45	theme	powder	747:752	arg1	weights					711:717	different weights	701:717	different weights of L. erythrorhizon extract powder	701:752	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	4	46	theme	powder	910:915	arg1	weights					899:905	different weights	889:905	different weights of powder	889:915	The concentrations of solution are 0.1mg/ml, 0.5mg/ml, 1.0mg/ml, and 2.0mg/ml and ethanol is chosen as the solvent, and different weights of powder are varied as 0.1mg, 1.0mg, 2.0mg, and 10mg.
25579910	2	47	dep	times	385:389	arg1	36h					402:404	36h	402:404	36h	402:404	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	47	dep	times	385:389	arg1	24					395:396	24	395:396	24	395:396	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	7	48	theme	wound	1327:1331	arg1	healing					1333:1339	the wound healing	1323:1339	the wound healing process	1323:1347	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	7	49	theme	viability	1198:1206	arg1	results					1182:1188	The results	1178:1188	The results of cell viability and animal study	1178:1223	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	7	50	theme	wound	1354:1358	arg1	reduction					1365:1373	the wound size reduction	1350:1373	the wound size reduction	1350:1373	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	3	51	theme	L.	722:723	arg1	powder					747:752	L. erythrorhizon extract powder	722:752	L. erythrorhizon extract powder	722:752	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	5	52	theme	L.	1020:1021	arg1	microcapsules					1037:1049	L. erythrorhizon microcapsules	1020:1049	L. erythrorhizon microcapsules	1020:1049	The cell viability test and animal study are performed on L. erythrorhizon microcapsules.
25579910	4	53	theme	solution	791:798	arg1	0.1mg/ml					804:811	0.1mg/ml	804:811	0.1mg/ml	804:811	The concentrations of solution are 0.1mg/ml, 0.5mg/ml, 1.0mg/ml, and 2.0mg/ml and ethanol is chosen as the solvent, and different weights of powder are varied as 0.1mg, 1.0mg, 2.0mg, and 10mg.
25579910	4	53	theme	solution	791:798	arg1	concentrations					773:786	The concentrations	769:786	The concentrations of solution	769:798	The concentrations of solution are 0.1mg/ml, 0.5mg/ml, 1.0mg/ml, and 2.0mg/ml and ethanol is chosen as the solvent, and different weights of powder are varied as 0.1mg, 1.0mg, 2.0mg, and 10mg.
25579910	2	54	theme	extraction	322:331	arg1	60°C					363:366	60°C	363:366	60°C	363:366	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	54	theme	extraction	322:331	arg1	40°C					347:350	40°C	347:350	40°C	347:350	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	54	theme	extraction	322:331	arg1	temperatures					333:344	extraction temperatures	322:344	extraction temperatures (40°C, 50°C, and 60°C)	322:367	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	54	theme	extraction	322:331	arg1	50°C					353:356	50°C	353:356	50°C	353:356	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	3	55	theme	erythrorhizon	725:737	arg1	powder					747:752	L. erythrorhizon extract powder	722:752	L. erythrorhizon extract powder	722:752	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	2	56	theme	study	219:223	arg1	extracts					225:232	This study extracts	214:232	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order	214:414	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	7	57	theme	cell	1193:1196	arg1	viability					1198:1206	cell viability	1193:1206	cell viability	1193:1206	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	5	58	theme	cell	966:969	arg1	viability					971:979	The cell viability test and animal study	962:1001	The cell viability test and animal study	962:1001	The cell viability test and animal study are performed on L. erythrorhizon microcapsules.
25579910	3	59	theme	extract	610:616	arg1	property					558:565	then the antibacterial property	535:565	then the antibacterial property	535:565	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	3	59	theme	extract	610:616	arg1	compatibility					576:588	cell compatibility	571:588	cell compatibility of L. erythrorhizon extract	571:616	The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively.
25579910	2	60	theme	solid-liquid	275:286	arg1	ratios					288:293	various solid-liquid ratios	267:293	various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12)	267:319	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	7	61	contain	have	1276:1279	arg2	viability					1291:1299	good cell viability	1281:1299	good cell viability (99%)	1281:1305	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	7	61	contain	have	1276:1279	arg1	SPL					1271:1273	SPL	1271:1273	SPL	1271:1273	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	7	61	contain	have	1276:1279	arg2	%					1304:1304	99%	1302:1304	99%	1302:1304	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	7	61	contain	have	1276:1279	arg1	microcapsules					1256:1268	the L. erythrorhizon microcapsules	1235:1268	the L. erythrorhizon microcapsules (SPL)	1235:1274	The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
25579910	0	62	theme	biological	60:69	arg1	evaluation					71:80	biological evaluation	60:80	biological evaluation of Lithospermum erythrorhizon microcapsules	60:124	The optimal extracting process, manufacturing technique and biological evaluation of Lithospermum erythrorhizon microcapsules.
25579910	5	63	theme	erythrorhizon	1023:1035	arg1	microcapsules					1037:1049	L. erythrorhizon microcapsules	1020:1049	L. erythrorhizon microcapsules	1020:1049	The cell viability test and animal study are performed on L. erythrorhizon microcapsules.
25579910	2	64	theme	various	267:273	arg1	ratios					288:293	various solid-liquid ratios	267:293	various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12)	267:319	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	65	from	times	385:389	arg1	ethanol					256:262	ethanol	256:262	ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order	256:414	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25579910	2	66	dep	extracts	225:232	arg1	erythrorhizon					237:249	erythrorhizon	237:249	erythrorhizon	237:249	This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters.
25526692	3	0	theme	adsorption	490:499	arg1	measurements					470:481	spectrophotometric measurements	451:481	spectrophotometric measurements of dye adsorption	451:499	Isotherm models were applied to the experimental equilibrium data obtained from spectrophotometric measurements of dye adsorption.
25526692	1	1	theme	Rhodamine	231:239	arg1	6G					241:242	Rhodamine 6G	231:242	Rhodamine 6G (Rh-6G)	231:250	The objective of this study was to investigate the use of chitosan-clay nanocomposite (CC) as an adsorbent for Rhodamine 6G (Rh-6G).
25526692	1	1	theme	Rhodamine	231:239	arg1	Rh-6G					245:249	Rh-6G	245:249	Rh-6G	245:249	The objective of this study was to investigate the use of chitosan-clay nanocomposite (CC) as an adsorbent for Rhodamine 6G (Rh-6G).
25526692	4	2	theme	Kinetic	510:516	arg1	models					518:523	Various Kinetic models	502:523	Various Kinetic models	502:523	Various Kinetic models were used to describe the kinetic data and evaluate of rate constants.
25526692	5	3	theme	optical	648:654	arg1	properties					674:683	their optical and photophysical properties	642:683	their optical and photophysical properties	642:683	Rh-6G loaded adsorbents were investigated for their optical and photophysical properties.
25526692	2	4	theme	concentration	302:314	arg1	effects					257:263	The effects	253:263	The effects of adsorbent dose, contact time, and concentration on the adsorption process	253:340	The effects of adsorbent dose, contact time, and concentration on the adsorption process were systematically studied.
25526692	0	5	theme	optical	100:106	arg1	properties					108:117	the resulting optical properties	86:117	the resulting optical properties	86:117	Chitosan, nanoclay and chitosan-nanoclay composite as adsorbents for Rhodamine-6G and the resulting optical properties.
25526692	4	6	theme	Various	502:508	arg1	models					518:523	Various Kinetic models	502:523	Various Kinetic models	502:523	Various Kinetic models were used to describe the kinetic data and evaluate of rate constants.
25526692	4	7	theme	rate	580:583	arg1	constants					585:593	rate constants	580:593	rate constants	580:593	Various Kinetic models were used to describe the kinetic data and evaluate of rate constants.
25526692	0	8	theme	resulting	90:98	arg1	properties					108:117	the resulting optical properties	86:117	the resulting optical properties	86:117	Chitosan, nanoclay and chitosan-nanoclay composite as adsorbents for Rhodamine-6G and the resulting optical properties.
25526692	5	9	theme	photophysical	660:672	arg1	properties					674:683	their optical and photophysical properties	642:683	their optical and photophysical properties	642:683	Rh-6G loaded adsorbents were investigated for their optical and photophysical properties.
25526692	5	10	theme	Rh-6G	596:600	arg1	adsorbents					609:618	Rh-6G loaded adsorbents	596:618	Rh-6G loaded adsorbents	596:618	Rh-6G loaded adsorbents were investigated for their optical and photophysical properties.
25526692	4	11	used	used	530:533	arg2	models					518:523	Various Kinetic models	502:523	Various Kinetic models	502:523	Various Kinetic models were used to describe the kinetic data and evaluate of rate constants.
25526692	5	12	theme	loaded	602:607	arg1	adsorbents					609:618	Rh-6G loaded adsorbents	596:618	Rh-6G loaded adsorbents	596:618	Rh-6G loaded adsorbents were investigated for their optical and photophysical properties.
25526692	3	13	theme	experimental	407:418	arg1	data					432:435	the experimental equilibrium data	403:435	the experimental equilibrium data obtained from spectrophotometric measurements of dye adsorption	403:499	Isotherm models were applied to the experimental equilibrium data obtained from spectrophotometric measurements of dye adsorption.
25526692	2	14	theme	dose	278:281	arg1	effects					257:263	The effects	253:263	The effects of adsorbent dose, contact time, and concentration on the adsorption process	253:340	The effects of adsorbent dose, contact time, and concentration on the adsorption process were systematically studied.
25526692	1	15	theme	chitosan-clay	178:190	arg1	CC					207:208	CC	207:208	CC	207:208	The objective of this study was to investigate the use of chitosan-clay nanocomposite (CC) as an adsorbent for Rhodamine 6G (Rh-6G).
25526692	1	15	theme	chitosan-clay	178:190	arg1	nanocomposite					192:204	chitosan-clay nanocomposite	178:204	chitosan-clay nanocomposite (CC)	178:209	The objective of this study was to investigate the use of chitosan-clay nanocomposite (CC) as an adsorbent for Rhodamine 6G (Rh-6G).
25526692	2	16	theme	adsorbent	268:276	arg1	dose					278:281	adsorbent dose	268:281	adsorbent dose	268:281	The effects of adsorbent dose, contact time, and concentration on the adsorption process were systematically studied.
25526692	1	17	theme	nanocomposite	192:204	arg1	use					171:173	the use	167:173	the use of chitosan-clay nanocomposite (CC) as an adsorbent for Rhodamine 6G (Rh-6G)	167:250	The objective of this study was to investigate the use of chitosan-clay nanocomposite (CC) as an adsorbent for Rhodamine 6G (Rh-6G).
25526692	1	18	theme	study	142:146	arg1	objective					124:132	The objective	120:132	The objective of this study	120:146	The objective of this study was to investigate the use of chitosan-clay nanocomposite (CC) as an adsorbent for Rhodamine 6G (Rh-6G).
25526692	4	19	theme	kinetic	551:557	arg1	data					559:562	the kinetic data	547:562	the kinetic data	547:562	Various Kinetic models were used to describe the kinetic data and evaluate of rate constants.
25526692	3	20	theme	equilibrium	420:430	arg1	data					432:435	the experimental equilibrium data	403:435	the experimental equilibrium data obtained from spectrophotometric measurements of dye adsorption	403:499	Isotherm models were applied to the experimental equilibrium data obtained from spectrophotometric measurements of dye adsorption.
25526692	3	21	theme	Isotherm	371:378	arg1	models					380:385	Isotherm models	371:385	Isotherm models	371:385	Isotherm models were applied to the experimental equilibrium data obtained from spectrophotometric measurements of dye adsorption.
25526692	3	22	theme	dye	486:488	arg1	adsorption					490:499	dye adsorption	486:499	dye adsorption	486:499	Isotherm models were applied to the experimental equilibrium data obtained from spectrophotometric measurements of dye adsorption.
25526692	2	23	theme	time	292:295	arg1	effects					257:263	The effects	253:263	The effects of adsorbent dose, contact time, and concentration on the adsorption process	253:340	The effects of adsorbent dose, contact time, and concentration on the adsorption process were systematically studied.
25526692	2	24	theme	contact	284:290	arg1	time					292:295	contact time	284:295	contact time	284:295	The effects of adsorbent dose, contact time, and concentration on the adsorption process were systematically studied.
25526692	2	25	from	effects	257:263	arg1	process					334:340	the adsorption process	319:340	the adsorption process	319:340	The effects of adsorbent dose, contact time, and concentration on the adsorption process were systematically studied.
25526692	3	26	theme	spectrophotometric	451:468	arg1	measurements					470:481	spectrophotometric measurements	451:481	spectrophotometric measurements of dye adsorption	451:499	Isotherm models were applied to the experimental equilibrium data obtained from spectrophotometric measurements of dye adsorption.
25526692	2	27	theme	adsorption	323:332	arg1	process					334:340	the adsorption process	319:340	the adsorption process	319:340	The effects of adsorbent dose, contact time, and concentration on the adsorption process were systematically studied.
26952474	4	0	theme	cytotoxic	815:823	arg1	effects					825:831	negative cytotoxic effects	806:831	negative cytotoxic effects towards the human osteosarcoma cell line MG-63	806:878	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis showed good antibacterial activity and exhibited negative cytotoxic effects towards the human osteosarcoma cell line MG-63.
26952474	0	1	theme	β-Ca3	72:76	arg1	coatings					110:117	Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings	63:117	Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings	63:117	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	4	2	theme	negative	806:813	arg1	effects					825:831	negative cytotoxic effects	806:831	negative cytotoxic effects towards the human osteosarcoma cell line MG-63	806:878	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis showed good antibacterial activity and exhibited negative cytotoxic effects towards the human osteosarcoma cell line MG-63.
26952474	2	3	theme	salient	449:455	arg1	powders					532:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	Silver, β-TCP and chitosan were combined to profit from their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders were synthesized by co-precipitation.
26952474	2	4	theme	antibacterial	475:487	arg1	powders					532:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	Silver, β-TCP and chitosan were combined to profit from their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders were synthesized by co-precipitation.
26952474	4	5	theme	2/chitosan	698:707	arg1	coatings					719:726	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings	676:726	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis	676:755	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis showed good antibacterial activity and exhibited negative cytotoxic effects towards the human osteosarcoma cell line MG-63.
26952474	5	6	theme	metallic	974:981	arg1	substrates					983:992	metallic substrates	974:992	metallic substrates	974:992	The morphology of the coatings was observed by SEM and their efficiency against corrosion of metallic substrates was determined through potentiodynamic polarization tests.
26952474	2	7	theme	complementary	461:473	arg1	powders					532:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	Silver, β-TCP and chitosan were combined to profit from their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders were synthesized by co-precipitation.
26952474	0	8	from	characteristics	44:58	arg1	metal					131:135	Titanium metal	122:135	Titanium metal	122:135	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	4	9	theme	Ag-dopedβ-Ca3	680:692	arg1	coatings					719:726	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings	676:726	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis	676:755	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis showed good antibacterial activity and exhibited negative cytotoxic effects towards the human osteosarcoma cell line MG-63.
26952474	1	10	theme	/chitosan	216:224	arg1	coatings					236:243	/chitosan composite coatings	216:243	/chitosan composite coatings	216:243	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	0	11	theme	hybrid	93:98	arg1	coatings					110:117	Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings	63:117	Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings	63:117	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	2	12	theme	features.The	507:518	arg1	powders					532:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	Silver, β-TCP and chitosan were combined to profit from their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders were synthesized by co-precipitation.
26952474	0	13	theme	2/chitosan	82:91	arg1	coatings					110:117	Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings	63:117	Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings	63:117	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	4	14	theme	antibacterial	769:781	arg1	activity					783:790	good antibacterial activity	764:790	good antibacterial activity	764:790	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis showed good antibacterial activity and exhibited negative cytotoxic effects towards the human osteosarcoma cell line MG-63.
26952474	3	15	theme	β-Ca3	663:667	arg1	lattice					652:658	the crystal lattice	640:658	the crystal lattice of β-Ca3(PO4)2	640:673	The characterization results confirmed the Ag(+) occupancy at the crystal lattice of β-Ca3(PO4)2.
26952474	1	16	dep	Pure	138:141	arg1	coatings					236:243	/chitosan composite coatings	216:243	/chitosan composite coatings	216:243	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	2	17	theme	biocompatible	493:505	arg1	powders					532:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	Silver, β-TCP and chitosan were combined to profit from their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders were synthesized by co-precipitation.
26952474	1	18	theme	composite	226:234	arg1	coatings					236:243	/chitosan composite coatings	216:243	/chitosan composite coatings	216:243	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	0	19	theme	coatings	110:117	arg1	Deposition					0:9	Deposition	0:9	Deposition	0:9	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	0	19	theme	coatings	110:117	arg1	characteristics					44:58	physical and invitro characteristics	23:58	physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal	23:135	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	0	20	theme	composite	100:108	arg1	coatings					110:117	Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings	63:117	Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings	63:117	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	5	21	theme	polarization	1033:1044	arg1	tests					1046:1050	potentiodynamic polarization tests	1017:1050	potentiodynamic polarization tests	1017:1050	The morphology of the coatings was observed by SEM and their efficiency against corrosion of metallic substrates was determined through potentiodynamic polarization tests.
26952474	5	22	theme	coatings	903:910	arg1	morphology					885:894	The morphology	881:894	The morphology of the coatings	881:910	The morphology of the coatings was observed by SEM and their efficiency against corrosion of metallic substrates was determined through potentiodynamic polarization tests.
26952474	1	23	theme	β-tricalcium	173:184	arg1	β-Ca3					204:208	β-Ca3(PO4)2	204:214	β-Ca3(PO4)2	204:214	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	1	23	theme	β-tricalcium	173:184	arg1	[β-TCP					196:201	β-tricalcium phosphate [β-TCP	173:201	β-tricalcium phosphate [β-TCP	173:201	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	0	24	theme	Titanium	122:129	arg1	metal					131:135	Titanium metal	122:135	Titanium metal	122:135	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	3	25	theme	Ag	621:622	arg1	occupancy					627:635	the Ag(+) occupancy	617:635	the Ag(+) occupancy	617:635	The characterization results confirmed the Ag(+) occupancy at the crystal lattice of β-Ca3(PO4)2.
26952474	1	26	theme	phosphate	186:194	arg1	β-Ca3					204:208	β-Ca3(PO4)2	204:214	β-Ca3(PO4)2	204:214	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	1	26	theme	phosphate	186:194	arg1	[β-TCP					196:201	β-tricalcium phosphate [β-TCP	173:201	β-tricalcium phosphate [β-TCP	173:201	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	0	27	theme	physical	23:30	arg1	Deposition					0:9	Deposition	0:9	Deposition	0:9	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	0	27	theme	physical	23:30	arg1	characteristics					44:58	physical and invitro characteristics	23:58	physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal	23:135	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	1	28	dep	0-5Ag	166:170	arg1	β-Ca3					204:208	β-Ca3(PO4)2	204:214	β-Ca3(PO4)2	204:214	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	1	28	dep	0-5Ag	166:170	arg1	[β-TCP					196:201	β-tricalcium phosphate [β-TCP	173:201	β-tricalcium phosphate [β-TCP	173:201	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	1	29	from	applications	331:342	arg1	replacements					359:370	hard tissue replacements	347:370	hard tissue replacements	347:370	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	1	30	theme	Titanium	263:270	arg1	substrates					277:286	Titanium (Ti) substrates	263:286	Titanium (Ti) substrates	263:286	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	4	31	theme	human	845:849	arg1	line					869:872	the human osteosarcoma cell line MG-63	841:878	the human osteosarcoma cell line MG-63	841:878	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis showed good antibacterial activity and exhibited negative cytotoxic effects towards the human osteosarcoma cell line MG-63.
26952474	1	32	theme	hard	347:350	arg1	replacements					359:370	hard tissue replacements	347:370	hard tissue replacements	347:370	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	4	33	theme	good	764:767	arg1	activity					783:790	good antibacterial activity	764:790	good antibacterial activity	764:790	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis showed good antibacterial activity and exhibited negative cytotoxic effects towards the human osteosarcoma cell line MG-63.
26952474	0	34	theme	invitro	36:42	arg1	Deposition					0:9	Deposition	0:9	Deposition	0:9	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	0	34	theme	invitro	36:42	arg1	characteristics					44:58	physical and invitro characteristics	23:58	physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal	23:135	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	2	35	theme	β-Ca3	520:524	arg1	powders					532:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders	443:538	Silver, β-TCP and chitosan were combined to profit from their salient and complementary antibacterial and biocompatible features.The β-Ca3(PO4)2 powders were synthesized by co-precipitation.
26952474	1	36	theme	tissue	352:357	arg1	replacements					359:370	hard tissue replacements	347:370	hard tissue replacements	347:370	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	5	37	theme	substrates	983:992	arg1	corrosion					961:969	corrosion	961:969	corrosion of metallic substrates	961:992	The morphology of the coatings was observed by SEM and their efficiency against corrosion of metallic substrates was determined through potentiodynamic polarization tests.
26952474	1	38	theme	Ti	273:274	arg1	substrates					277:286	Titanium (Ti) substrates	263:286	Titanium (Ti) substrates	263:286	Pure and five silver-doped (0-5Ag) β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2]/chitosan composite coatings were deposited on Titanium (Ti) substrates and their properties that are relevant for applications in hard tissue replacements were assessed.
26952474	4	39	theme	cell	864:867	arg1	line					869:872	the human osteosarcoma cell line MG-63	841:878	the human osteosarcoma cell line MG-63	841:878	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis showed good antibacterial activity and exhibited negative cytotoxic effects towards the human osteosarcoma cell line MG-63.
26952474	4	40	theme	composite	709:717	arg1	coatings					719:726	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings	676:726	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis	676:755	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis showed good antibacterial activity and exhibited negative cytotoxic effects towards the human osteosarcoma cell line MG-63.
26952474	4	41	theme	osteosarcoma	851:862	arg1	line					869:872	the human osteosarcoma cell line MG-63	841:878	the human osteosarcoma cell line MG-63	841:878	The Ag-dopedβ-Ca3(PO4)2/chitosan composite coatings deposited by electrophoresis showed good antibacterial activity and exhibited negative cytotoxic effects towards the human osteosarcoma cell line MG-63.
26952474	3	42	theme	characterization	582:597	arg1	results					599:605	The characterization results	578:605	The characterization results	578:605	The characterization results confirmed the Ag(+) occupancy at the crystal lattice of β-Ca3(PO4)2.
26952474	0	43	theme	Ag-doped	63:70	arg1	coatings					110:117	Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings	63:117	Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings	63:117	Deposition, structure, physical and invitro characteristics of Ag-doped β-Ca3(PO4)2/chitosan hybrid composite coatings on Titanium metal.
26952474	5	44	theme	potentiodynamic	1017:1031	arg1	tests					1046:1050	potentiodynamic polarization tests	1017:1050	potentiodynamic polarization tests	1017:1050	The morphology of the coatings was observed by SEM and their efficiency against corrosion of metallic substrates was determined through potentiodynamic polarization tests.
26952474	3	45	theme	crystal	644:650	arg1	lattice					652:658	the crystal lattice	640:658	the crystal lattice of β-Ca3(PO4)2	640:673	The characterization results confirmed the Ag(+) occupancy at the crystal lattice of β-Ca3(PO4)2.
28619640	0	0	theme	Cr	85:86	arg1	biosorption					64:74	biosorption	64:74	biosorption of toxic Cr (VI)	64:91	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	6	1	theme	Langmuir	1182:1189	arg1	model					1191:1195	Langmuir model	1182:1195	Langmuir model	1182:1195	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	6	2	dep	R2	983:984	arg1	0.76					993:996	0.76	993:996	0.76	993:996	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	6	2	dep	R2	983:984	arg1	0.50					987:990	0.50	987:990	0.50	987:990	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	4	3	theme	kinetic	651:657	arg1	model					659:663	pseudo-second order kinetic model	631:663	pseudo-second order kinetic model	631:663	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	1	4	theme	toxic	216:220	arg1	Cr					222:223	toxic Cr	216:223	toxic Cr (VI)	216:228	In this work, amine riched MnFe2O4-cellulose was employed to remove toxic Cr (VI).
28619640	0	5	theme	toxic	79:83	arg1	Cr					85:86	toxic Cr	79:86	toxic Cr (VI)	79:91	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	6	6	theme	adsorption	792:801	arg1	parameters					812:821	The adsorption isotherm parameters	788:821	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC	788:861	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	6	7	theme	isotherm	934:941	arg1	more					946:949	more	946:949	more	946:949	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	6	7	theme	isotherm	934:941	arg1	R2					904:905	R2	904:905	R2	904:905	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	6	7	theme	isotherm	934:941	arg1	coefficient					891:901	the correlation coefficient	875:901	the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm	875:941	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	4	8	theme	order	645:649	arg1	model					659:663	pseudo-second order kinetic model	631:663	pseudo-second order kinetic model	631:663	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	6	9	contain	possessing	1095:1104	arg1	multilayer					1084:1093	multilayer	1084:1093	multilayer possessing heterogeneous sorption sites	1084:1133	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	6	9	contain	possessing	1095:1104	arg2	sites					1129:1133	heterogeneous sorption sites	1106:1133	heterogeneous sorption sites	1106:1133	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	4	10	theme	magnetic	493:500	arg1	MC					513:514	MC	513:514	MC	513:514	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	4	10	theme	magnetic	493:500	arg1	cellulose					502:510	magnetic cellulose	493:510	magnetic cellulose (MC)	493:515	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	7	11	theme	adsorption	1210:1219	arg1	capacities					1221:1230	The maximum adsorption capacities	1198:1230	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions	1198:1281	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	7	11	theme	adsorption	1210:1219	arg1	25					1300:1301	25	1300:1301	25	1300:1301	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	5	12	theme	diffusion	698:706	arg1	results					714:720	the obtained intraparticle diffusion model results	671:720	the obtained intraparticle diffusion model results	671:720	From the obtained intraparticle diffusion model results it is evident that the adsorption is an external surface process.
28619640	6	13	dep	R2	904:905	arg1	0.96					908:911	0.96	908:911	0.96	908:911	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	6	13	dep	R2	904:905	arg1	0.88					914:917	0.88	914:917	0.88	914:917	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	2	14	used	used	289:292	arg2	software					276:283	R (3.3.1) software	266:283	R (3.3.1) software	266:283	To find out the optimum conditions R (3.3.1) software was used.
28619640	4	15	theme	pseudo-second	631:643	arg1	model					659:663	pseudo-second order kinetic model	631:663	pseudo-second order kinetic model	631:663	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	3	16	theme	isotherm	398:405	arg1	studies					407:413	kinetic and isotherm studies	386:413	studies	407:413	The results of Cr (VI) adsorption onto three-step adsorbents were compared by interpreting kinetic and isotherm studies.
28619640	5	17	from	results	714:720	arg1	evident					728:734	evident	728:734	evident	728:734	From the obtained intraparticle diffusion model results it is evident that the adsorption is an external surface process.
28619640	6	18	theme	isotherm	803:810	arg1	parameters					812:821	The adsorption isotherm parameters	788:821	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC	788:861	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	1	19	theme	amine	162:166	arg1	MnFe2O4-cellulose					175:191	amine riched MnFe2O4-cellulose	162:191	amine riched MnFe2O4-cellulose	162:191	In this work, amine riched MnFe2O4-cellulose was employed to remove toxic Cr (VI).
28619640	6	20	theme	Freundlich	923:932	arg1	isotherm					934:941	Freundlich isotherm	923:941	Freundlich isotherm	923:941	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	5	21	theme	obtained	675:682	arg1	results					714:720	the obtained intraparticle diffusion model results	671:720	the obtained intraparticle diffusion model results	671:720	From the obtained intraparticle diffusion model results it is evident that the adsorption is an external surface process.
28619640	2	22	dep	used	289:292	arg1	find					234:237	find	234:237	To find out the optimum conditions	231:264	To find out the optimum conditions R (3.3.1) software was used.
28619640	7	23	theme	AFMC	1253:1256	arg1	capacities					1221:1230	The maximum adsorption capacities	1198:1230	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions	1198:1281	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	7	23	theme	AFMC	1253:1256	arg1	25					1300:1301	25	1300:1301	25	1300:1301	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	5	24	theme	intraparticle	684:696	arg1	results					714:720	the obtained intraparticle diffusion model results	671:720	the obtained intraparticle diffusion model results	671:720	From the obtained intraparticle diffusion model results it is evident that the adsorption is an external surface process.
28619640	4	25	dep	Cr	466:467	arg1	VI					470:471	VI	470:471	VI	470:471	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	6	26	theme	Cr	1046:1047	arg1	adsorption					1032:1041	the adsorption	1028:1041	the adsorption of Cr	1028:1047	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	0	27	dep	Cr	85:86	arg1	VI					89:90	VI	89:90	VI	89:90	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	7	28	theme	optimum	1264:1270	arg1	conditions					1272:1281	optimum conditions	1264:1281	optimum conditions	1264:1281	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	3	29	theme	Cr	310:311	arg1	adsorption					318:327	Cr (VI) adsorption	310:327	Cr (VI) adsorption onto three-step adsorbents	310:354	The results of Cr (VI) adsorption onto three-step adsorbents were compared by interpreting kinetic and isotherm studies.
28619640	3	30	dep	adsorption	318:327	arg1	VI					314:315	VI	314:315	VI	314:315	The results of Cr (VI) adsorption onto three-step adsorbents were compared by interpreting kinetic and isotherm studies.
28619640	2	31	theme	optimum	247:253	arg1	conditions					255:264	the optimum conditions	243:264	the optimum conditions	243:264	To find out the optimum conditions R (3.3.1) software was used.
28619640	3	32	theme	three-step	334:343	arg1	adsorbents					345:354	three-step adsorbents	334:354	three-step adsorbents	334:354	The results of Cr (VI) adsorption onto three-step adsorbents were compared by interpreting kinetic and isotherm studies.
28619640	0	33	theme	nano-structured	26:40	arg1	cellulose					50:58	amino-riched nano-structured MnFe2O4@cellulose	13:58	amino-riched nano-structured MnFe2O4@cellulose	13:58	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	4	34	theme	Cr	466:467	arg1	adsorption					452:461	the adsorption	448:461	the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC)	448:515	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	6	35	dep	occurs	1074:1079	arg1	VI					1050:1051	VI	1050:1051	VI	1050:1051	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	4	36	theme	pseudo-first	526:537	arg1	model					545:549	pseudo-first order model	526:549	pseudo-first order model	526:549	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	0	37	theme	amino-riched	13:24	arg1	cellulose					50:58	amino-riched nano-structured MnFe2O4@cellulose	13:58	amino-riched nano-structured MnFe2O4@cellulose	13:58	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	6	38	dep	Cr	827:828	arg1	adsorption					835:844	adsorption	835:844	Cr (VI) adsorption onto MC and AFMC	827:861	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	6	38	dep	Cr	827:828	arg1	VI					831:832	VI	831:832	VI	831:832	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	7	39	theme	maximum	1202:1208	arg1	capacities					1221:1230	The maximum adsorption capacities	1198:1230	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions	1198:1281	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	7	39	theme	maximum	1202:1208	arg1	25					1300:1301	25	1300:1301	25	1300:1301	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	4	40	dep	Cr	577:578	arg1	VI					581:582	VI	581:582	VI	581:582	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	3	41	theme	kinetic	386:392	arg1	studies					407:413	kinetic and isotherm studies	386:413	studies	407:413	The results of Cr (VI) adsorption onto three-step adsorbents were compared by interpreting kinetic and isotherm studies.
28619640	0	42	theme	@	49:49	arg1	cellulose					50:58	amino-riched nano-structured MnFe2O4@cellulose	13:58	amino-riched nano-structured MnFe2O4@cellulose	13:58	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	7	43	theme	modification	1408:1419	arg1	steps					1421:1425	modification steps	1408:1425	modification steps	1408:1425	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	6	44	theme	sorption	1120:1127	arg1	sites					1129:1133	heterogeneous sorption sites	1106:1133	heterogeneous sorption sites	1106:1133	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	7	45	theme	MC	1246:1247	arg1	capacities					1221:1230	The maximum adsorption capacities	1198:1230	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions	1198:1281	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	7	45	theme	MC	1246:1247	arg1	25					1300:1301	25	1300:1301	25	1300:1301	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	1	46	theme	riched	168:173	arg1	MnFe2O4-cellulose					175:191	amine riched MnFe2O4-cellulose	162:191	amine riched MnFe2O4-cellulose	162:191	In this work, amine riched MnFe2O4-cellulose was employed to remove toxic Cr (VI).
28619640	0	47	theme	MnFe2O4	42:48	arg1	cellulose					50:58	amino-riched nano-structured MnFe2O4@cellulose	13:58	amino-riched nano-structured MnFe2O4@cellulose	13:58	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	3	48	theme	adsorption	318:327	arg1	results					299:305	The results	295:305	The results of Cr (VI) adsorption onto three-step adsorbents	295:354	The results of Cr (VI) adsorption onto three-step adsorbents were compared by interpreting kinetic and isotherm studies.
28619640	5	49	theme	model	708:712	arg1	results					714:720	the obtained intraparticle diffusion model results	671:720	the obtained intraparticle diffusion model results	671:720	From the obtained intraparticle diffusion model results it is evident that the adsorption is an external surface process.
28619640	4	50	theme	order	539:543	arg1	model					545:549	pseudo-first order model	526:549	pseudo-first order model	526:549	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	5	51	theme	external	762:769	arg1	process					779:785	an external surface process	759:785	an external surface process	759:785	From the obtained intraparticle diffusion model results it is evident that the adsorption is an external surface process.
28619640	5	51	theme	external	762:769	arg1	adsorption					745:754	the adsorption	741:754	the adsorption	741:754	From the obtained intraparticle diffusion model results it is evident that the adsorption is an external surface process.
28619640	7	52	theme	adsorption	1362:1371	arg1	efficiency					1373:1382	adsorption efficiency	1362:1382	adsorption efficiency	1362:1382	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	4	53	theme	amine	590:594	arg1	AFMC					616:619	AFMC	616:619	AFMC	616:619	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	4	53	theme	amine	590:594	arg1	cellulose					605:613	amine modified cellulose	590:613	amine modified cellulose (AFMC)	590:620	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	0	54	theme	cellulose	50:58	arg1	Potential					0:8	Potential	0:8	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.	0:146	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	6	55	dep	more	946:949	arg1	R2					983:984	R2	983:984	R2	983:984	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	6	56	theme	Langmuir	964:971	arg1	isotherm					973:980	Langmuir isotherm	964:980	Langmuir isotherm	964:980	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	7	57	theme	cellulose	1235:1243	arg1	capacities					1221:1230	The maximum adsorption capacities	1198:1230	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions	1198:1281	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	7	57	theme	cellulose	1235:1243	arg1	25					1300:1301	25	1300:1301	25	1300:1301	The maximum adsorption capacities of cellulose, MC and AFMC under optimum conditions were found to be 25, 125 and 250mg/g, respectively; thus, it is confirmed that adsorption efficiency has been improved after modification steps.
28619640	6	58	theme	heterogeneous	1106:1118	arg1	sites					1129:1133	heterogeneous sorption sites	1106:1133	heterogeneous sorption sites	1106:1133	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	0	59	theme	comparing	129:137	arg1	Modeling					94:101	Modeling	94:101	Modeling	94:101	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	0	59	theme	comparing	129:137	arg1	studies					139:145	comparing studies	129:145	comparing studies	129:145	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	2	60	theme	R	266:266	arg1	software					276:283	R (3.3.1) software	266:283	R (3.3.1) software	266:283	To find out the optimum conditions R (3.3.1) software was used.
28619640	4	61	theme	modified	596:603	arg1	AFMC					616:619	AFMC	616:619	AFMC	616:619	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	4	61	theme	modified	596:603	arg1	cellulose					605:613	amine modified cellulose	590:613	amine modified cellulose (AFMC)	590:620	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	1	62	dep	Cr	222:223	arg1	VI					226:227	VI	226:227	VI	226:227	In this work, amine riched MnFe2O4-cellulose was employed to remove toxic Cr (VI).
28619640	5	63	theme	surface	771:777	arg1	process					779:785	an external surface process	759:785	an external surface process	759:785	From the obtained intraparticle diffusion model results it is evident that the adsorption is an external surface process.
28619640	5	63	theme	surface	771:777	arg1	adsorption					745:754	the adsorption	741:754	the adsorption	741:754	From the obtained intraparticle diffusion model results it is evident that the adsorption is an external surface process.
28619640	6	64	theme	correlation	879:889	arg1	more					946:949	more	946:949	more	946:949	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	6	64	theme	correlation	879:889	arg1	R2					904:905	R2	904:905	R2	904:905	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	6	64	theme	correlation	879:889	arg1	coefficient					891:901	the correlation coefficient	875:901	the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm	875:941	The adsorption isotherm parameters for Cr (VI) adsorption onto MC and AFMC showed that the correlation coefficient (R2: 0.96, 0.88) of Freundlich isotherm is more than that of Langmuir isotherm (R2: 0.50, 0.76); hence, it is suggested that the adsorption of Cr (VI) onto these sorbents occurs in multilayer possessing heterogeneous sorption sites whereas the adsorption onto cellulose followed Langmuir model.
28619640	4	65	theme	kinetic	420:426	arg1	studies					428:434	The kinetic studies	416:434	The kinetic studies	416:434	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28619640	0	66	dep	Potential	0:8	arg1	kinetic					104:110	kinetic	104:110	kinetic	104:110	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	0	66	dep	Potential	0:8	arg1	Modeling					94:101	Modeling	94:101	Modeling	94:101	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	0	66	dep	Potential	0:8	arg1	equilibrium					113:123	equilibrium	113:123	equilibrium	113:123	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	0	66	dep	Potential	0:8	arg1	studies					139:145	comparing studies	129:145	comparing studies	129:145	Potential of amino-riched nano-structured MnFe2O4@cellulose for biosorption of toxic Cr (VI): Modeling, kinetic, equilibrium and comparing studies.
28619640	4	67	theme	Cr	577:578	arg1	adsorption					563:572	the adsorption	559:572	the adsorption of Cr (VI) onto amine modified cellulose (AFMC)	559:620	The kinetic studies reveal that the adsorption of Cr (VI) onto cellulose and magnetic cellulose (MC) followed pseudo-first order model whereas the adsorption of Cr (VI) onto amine modified cellulose (AFMC) followed pseudo-second order kinetic model.
28064051	0	0	theme	cane	46:49	arg1	cellulose					59:67	sugar cane bagasse cellulose	40:67	sugar cane bagasse cellulose	40:67	Removal of Cu (II) ion from water using sugar cane bagasse cellulose and gelatin based composite hydrogels.
28064051	2	1	theme	pH	395:396	arg1	charge					412:417	pH at point zero charge	395:417	pH at point zero charge	395:417	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	0	2	theme	sugar	40:44	arg1	cellulose					59:67	sugar cane bagasse cellulose	40:67	sugar cane bagasse cellulose	40:67	Removal of Cu (II) ion from water using sugar cane bagasse cellulose and gelatin based composite hydrogels.
28064051	3	3	theme	resulting	550:558	arg1	hydrogels					560:568	the resulting hydrogels	546:568	the resulting hydrogels	546:568	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	3	4	with	methodology	634:644	arg1	design					669:674	Box-Behnken design	657:674	Box-Behnken design (BBD)	657:680	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	3	4	with	methodology	634:644	arg1	BBD					677:679	BBD	677:679	BBD	677:679	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	4	5	theme	process	785:791	arg1	conditions					793:802	varied process conditions	778:802	varied process conditions	778:802	The hydrogel prepared with optimized parameters was further studied for sorption of Cu (II) at varied process conditions.
28064051	1	6	theme	free	289:292	arg1	radical					294:300	free radical	289:300	free radical solution polymerization	289:324	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	3	7	from	water	537:541	arg1	sorption					512:519	sorption	512:519	sorption of Cu (II) from water by the resulting hydrogels	512:568	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	1	8	theme	natural	217:223	arg1	GT					242:243	GT	242:243	GT	242:243	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	1	8	theme	natural	217:223	arg1	gelatin					233:239	a natural polymer gelatin	215:239	a natural polymer gelatin (GT)	215:244	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	0	9	theme	bagasse	51:57	arg1	cellulose					59:67	sugar cane bagasse cellulose	40:67	sugar cane bagasse cellulose	40:67	Removal of Cu (II) ion from water using sugar cane bagasse cellulose and gelatin based composite hydrogels.
28064051	1	10	theme	radical	294:300	arg1	polymerization					311:324	free radical solution polymerization	289:324	free radical solution polymerization	289:324	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	1	11	theme	polymer	225:231	arg1	GT					242:243	GT	242:243	GT	242:243	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	1	11	theme	polymer	225:231	arg1	gelatin					233:239	a natural polymer gelatin	215:239	a natural polymer gelatin (GT)	215:244	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	1	12	theme	solution	302:309	arg1	polymerization					311:324	free radical solution polymerization	289:324	free radical solution polymerization	289:324	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	4	13	theme	optimized	710:718	arg1	parameters					720:729	optimized parameters	710:729	optimized parameters	710:729	The hydrogel prepared with optimized parameters was further studied for sorption of Cu (II) at varied process conditions.
28064051	3	14	theme	response	617:624	arg1	RSM					647:649	RSM	647:649	RSM	647:649	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	3	14	theme	response	617:624	arg1	methodology					634:644	response surface methodology	617:644	response surface methodology (RSM) with Box-Behnken design (BBD)	617:680	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	1	15	theme	in	153:154	arg1	incorporation					161:173	in situ incorporation	153:173	in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization	153:324	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	1	16	theme	gelatin	233:239	arg1	incorporation					161:173	in situ incorporation	153:173	in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization	153:324	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	3	17	from	effects	473:479	arg1	sorption					512:519	sorption	512:519	sorption of Cu (II) from water by the resulting hydrogels	512:568	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	3	18	theme	surface	626:632	arg1	RSM					647:649	RSM	647:649	RSM	647:649	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	3	18	theme	surface	626:632	arg1	methodology					634:644	response surface methodology	617:644	response surface methodology (RSM) with Box-Behnken design (BBD)	617:680	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	0	19	theme	composite	87:95	arg1	hydrogels					97:105	composite hydrogels	87:105	composite hydrogels	87:105	Removal of Cu (II) ion from water using sugar cane bagasse cellulose and gelatin based composite hydrogels.
28064051	2	20	dep	FTIR	363:366	arg1	reversibility					432:444	reversibility	432:444	reversibility	432:444	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	0	21	theme	Cu	11:12	arg1	ion					19:21	Cu (II) ion	11:21	Cu (II) ion	11:21	Removal of Cu (II) ion from water using sugar cane bagasse cellulose and gelatin based composite hydrogels.
28064051	2	22	theme	varied	449:454	arg1	pH					456:457	varied pH	449:457	varied pH in water	449:466	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	4	23	theme	varied	778:783	arg1	conditions					793:802	varied process conditions	778:802	varied process conditions	778:802	The hydrogel prepared with optimized parameters was further studied for sorption of Cu (II) at varied process conditions.
28064051	1	24	dep	in	153:154	arg1	situ					156:159	situ	156:159	situ	156:159	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	1	25	theme	sugarcane	178:186	arg1	CB					207:208	CB	207:208	CB	207:208	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	1	25	theme	sugarcane	178:186	arg1	cellulose					196:204	sugarcane bagasse cellulose	178:204	sugarcane bagasse cellulose (CB)	178:209	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	3	26	theme	Cu	524:525	arg1	sorption					512:519	sorption	512:519	sorption of Cu (II) from water by the resulting hydrogels	512:568	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	3	27	theme	synthesis	488:496	arg1	parameters					498:507	the synthesis parameters	484:507	the synthesis parameters	484:507	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	1	28	theme	bagasse	188:194	arg1	CB					207:208	CB	207:208	CB	207:208	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	1	28	theme	bagasse	188:194	arg1	cellulose					196:204	sugarcane bagasse cellulose	178:204	sugarcane bagasse cellulose (CB)	178:209	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	0	29	from	water	28:32	arg1	Removal					0:6	Removal	0:6	Removal of Cu (II) ion from water using sugar cane bagasse cellulose	0:67	Removal of Cu (II) ion from water using sugar cane bagasse cellulose and gelatin based composite hydrogels.
28064051	0	29	from	water	28:32	arg1	gelatin					73:79	gelatin	73:79	gelatin	73:79	Removal of Cu (II) ion from water using sugar cane bagasse cellulose and gelatin based composite hydrogels.
28064051	0	30	theme	ion	19:21	arg1	Removal					0:6	Removal	0:6	Removal of Cu (II) ion from water using sugar cane bagasse cellulose	0:67	Removal of Cu (II) ion from water using sugar cane bagasse cellulose and gelatin based composite hydrogels.
28064051	0	30	theme	ion	19:21	arg1	gelatin					73:79	gelatin	73:79	gelatin	73:79	Removal of Cu (II) ion from water using sugar cane bagasse cellulose and gelatin based composite hydrogels.
28064051	2	31	from	charge	412:417	arg1	at					398:399	pH at point zero charge	395:417	pH at point zero charge	395:417	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	2	31	from	charge	412:417	arg1	point					401:405	pH at point zero charge	395:417	pH at point zero charge	395:417	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	2	31	from	charge	412:417	arg1	pH					395:396	pH at point zero charge	395:417	pH at point zero charge	395:417	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	1	32	theme	polyacrylic	256:266	arg1	network					278:284	the polyacrylic copolymer network	252:284	the polyacrylic copolymer network	252:284	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	4	33	theme	Cu	767:768	arg1	sorption					755:762	sorption	755:762	sorption of Cu (II)	755:773	The hydrogel prepared with optimized parameters was further studied for sorption of Cu (II) at varied process conditions.
28064051	1	34	theme	cellulose	196:204	arg1	incorporation					161:173	in situ incorporation	153:173	in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization	153:324	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	2	35	theme	point	401:405	arg1	charge					412:417	pH at point zero charge	395:417	pH at point zero charge	395:417	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	3	36	theme	parameters	498:507	arg1	effects					473:479	The effects	469:479	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels	469:568	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	1	37	theme	Several	108:114	arg1	hydrogels					126:134	Several composite hydrogels	108:134	Several composite hydrogels	108:134	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	2	38	from	pH	456:457	arg1	charge					412:417	pH at point zero charge	395:417	pH at point zero charge	395:417	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	2	38	from	pH	456:457	arg1	TGA					374:376	TGA	374:376	TGA	374:376	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	2	38	from	pH	456:457	arg1	swelling					423:430	swelling	423:430	swelling	423:430	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	2	38	from	pH	456:457	arg1	water					462:466	water	462:466	water	462:466	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	2	38	from	pH	456:457	arg1	DMA					379:381	DMA	379:381	DMA	379:381	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	2	38	from	pH	456:457	arg1	FTIR					363:366	FTIR	363:366	FTIR	363:366	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	2	38	from	pH	456:457	arg1	XRD					369:371	XRD	369:371	XRD	369:371	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	2	38	from	pH	456:457	arg1	SEM					384:386	SEM	384:386	SEM	384:386	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	2	38	from	pH	456:457	arg1	EDAX					389:392	EDAX	389:392	EDAX	389:392	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	2	39	theme	at	398:399	arg1	charge					412:417	pH at point zero charge	395:417	pH at point zero charge	395:417	The hydrogels were characterized by FTIR, XRD, TGA, DMA, SEM, EDAX, pH at point zero charge and swelling reversibility at varied pH in water.
28064051	1	40	theme	copolymer	268:276	arg1	network					278:284	the polyacrylic copolymer network	252:284	the polyacrylic copolymer network	252:284	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	1	41	theme	composite	116:124	arg1	hydrogels					126:134	Several composite hydrogels	108:134	Several composite hydrogels	108:134	Several composite hydrogels were prepared by in situ incorporation of sugarcane bagasse cellulose (CB) and a natural polymer gelatin (GT) in to the polyacrylic copolymer network by free radical solution polymerization.
28064051	3	42	theme	Box-Behnken	657:667	arg1	design					669:674	Box-Behnken design	657:674	Box-Behnken design (BBD)	657:680	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28064051	3	42	theme	Box-Behnken	657:667	arg1	BBD					677:679	BBD	677:679	BBD	677:679	The effects of the synthesis parameters on sorption of Cu (II) from water by the resulting hydrogels were studied and the results were optimized by response surface methodology (RSM) with Box-Behnken design (BBD).
28692807	0	0	from	effect	4:9	arg1	tolerance					74:82	glucose tolerance	66:82	glucose tolerance	66:82	The effect of moderate consumption of non-nutritive sweeteners on glucose tolerance and body composition in rats.
28692807	0	0	from	effect	4:9	arg1	composition					93:103	body composition	88:103	body composition	88:103	The effect of moderate consumption of non-nutritive sweeteners on glucose tolerance and body composition in rats.
28692807	3	1	contain	had	361:363	arg1	treatment					351:359	NNS treatment	347:359	NNS treatment	347:359	NNS treatment had no effect on weight gain or percent body fat.
28692807	3	1	contain	had	361:363	arg2	effect					368:373	no effect	365:373	no effect	365:373	NNS treatment had no effect on weight gain or percent body fat.
28692807	2	2	with	glucose	289:295	arg1	NNS					314:316	NNS	314:316	NNS	314:316	Areas under the curve for glucose and insulin with NNS did not differ from control.
28692807	0	3	theme	body	88:91	arg1	composition					93:103	body composition	88:103	body composition	88:103	The effect of moderate consumption of non-nutritive sweeteners on glucose tolerance and body composition in rats.
28692807	4	4	theme	pad	426:428	arg1	mass					430:433	Epididymal fat pad mass	411:433	Epididymal fat pad mass	411:433	Epididymal fat pad mass was higher with aspartame and the ratio of trunk to total fat was less with sucralose versus control, suggesting that NNS consumption altered body fat distribution.
28692807	1	5	theme	drinking	247:254	arg1	water					256:260	drinking water	247:260	drinking water	247:260	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28692807	4	6	theme	body	577:580	arg1	fat					582:584	body fat	577:584	body fat distribution	577:597	Epididymal fat pad mass was higher with aspartame and the ratio of trunk to total fat was less with sucralose versus control, suggesting that NNS consumption altered body fat distribution.
28692807	1	7	theme	male	172:175	arg1	rats					177:180	male rats	172:180	male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water	172:260	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28692807	3	8	theme	NNS	347:349	arg1	treatment					351:359	NNS treatment	347:359	NNS treatment	347:359	NNS treatment had no effect on weight gain or percent body fat.
28692807	4	9	theme	fat	582:584	arg1	distribution					586:597	body fat distribution	577:597	body fat distribution	577:597	Epididymal fat pad mass was higher with aspartame and the ratio of trunk to total fat was less with sucralose versus control, suggesting that NNS consumption altered body fat distribution.
28692807	4	10	theme	fat	422:424	arg1	mass					430:433	Epididymal fat pad mass	411:433	Epididymal fat pad mass	411:433	Epididymal fat pad mass was higher with aspartame and the ratio of trunk to total fat was less with sucralose versus control, suggesting that NNS consumption altered body fat distribution.
28692807	3	11	theme	weight	378:383	arg1	gain					385:388	weight gain	378:388	weight gain	378:388	NNS treatment had no effect on weight gain or percent body fat.
28692807	4	12	theme	total	487:491	arg1	fat					493:495	total fat	487:495	total fat	487:495	Epididymal fat pad mass was higher with aspartame and the ratio of trunk to total fat was less with sucralose versus control, suggesting that NNS consumption altered body fat distribution.
28692807	0	13	theme	moderate	14:21	arg1	consumption					23:33	moderate consumption	14:33	moderate consumption of non-nutritive sweeteners	14:61	The effect of moderate consumption of non-nutritive sweeteners on glucose tolerance and body composition in rats.
28692807	1	14	theme	non-nutritive	188:200	arg1	aspartame					220:228	aspartame	220:228	aspartame	220:228	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28692807	1	14	theme	non-nutritive	188:200	arg1	sweeteners					202:211	non-nutritive sweeteners	188:211	non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water	188:260	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28692807	1	14	theme	non-nutritive	188:200	arg1	sucralose					233:241	sucralose	233:241	sucralose	233:241	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28692807	1	14	theme	non-nutritive	188:200	arg1	NNS					214:216	NNS	214:216	NNS	214:216	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28692807	2	15	with	insulin	301:307	arg1	NNS					314:316	NNS	314:316	NNS	314:316	Areas under the curve for glucose and insulin with NNS did not differ from control.
28692807	4	16	theme	NNS	553:555	arg1	consumption					557:567	NNS consumption	553:567	NNS consumption	553:567	Epididymal fat pad mass was higher with aspartame and the ratio of trunk to total fat was less with sucralose versus control, suggesting that NNS consumption altered body fat distribution.
28692807	0	17	from	tolerance	74:82	arg1	rats					108:111	rats	108:111	rats	108:111	The effect of moderate consumption of non-nutritive sweeteners on glucose tolerance and body composition in rats.
28692807	3	18	theme	body	401:404	arg1	fat					406:408	percent body fat	393:408	percent body fat	393:408	NNS treatment had no effect on weight gain or percent body fat.
28692807	0	19	theme	consumption	23:33	arg1	effect					4:9	The effect	0:9	The effect of moderate consumption of non-nutritive sweeteners on glucose tolerance and body composition in rats	0:111	The effect of moderate consumption of non-nutritive sweeteners on glucose tolerance and body composition in rats.
28692807	4	20	theme	Epididymal	411:420	arg1	mass					430:433	Epididymal fat pad mass	411:433	Epididymal fat pad mass	411:433	Epididymal fat pad mass was higher with aspartame and the ratio of trunk to total fat was less with sucralose versus control, suggesting that NNS consumption altered body fat distribution.
28692807	0	21	theme	sweeteners	52:61	arg1	consumption					23:33	moderate consumption	14:33	moderate consumption of non-nutritive sweeteners	14:61	The effect of moderate consumption of non-nutritive sweeteners on glucose tolerance and body composition in rats.
28692807	0	22	theme	non-nutritive	38:50	arg1	sweeteners					52:61	non-nutritive sweeteners	38:61	non-nutritive sweeteners	38:61	The effect of moderate consumption of non-nutritive sweeteners on glucose tolerance and body composition in rats.
28692807	4	23	theme	trunk	478:482	arg1	less					501:504	less	501:504	less	501:504	Epididymal fat pad mass was higher with aspartame and the ratio of trunk to total fat was less with sucralose versus control, suggesting that NNS consumption altered body fat distribution.
28692807	4	23	theme	trunk	478:482	arg1	ratio					469:473	the ratio	465:473	the ratio of trunk to total fat	465:495	Epididymal fat pad mass was higher with aspartame and the ratio of trunk to total fat was less with sucralose versus control, suggesting that NNS consumption altered body fat distribution.
28692807	1	24	theme	Glucose	114:120	arg1	tolerance					122:130	Glucose tolerance	114:130	Glucose tolerance	114:130	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28692807	1	25	from	sweeteners	202:211	arg1	water					256:260	drinking water	247:260	drinking water	247:260	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28692807	0	26	from	composition	93:103	arg1	rats					108:111	rats	108:111	rats	108:111	The effect of moderate consumption of non-nutritive sweeteners on glucose tolerance and body composition in rats.
28692807	0	27	theme	glucose	66:72	arg1	tolerance					74:82	glucose tolerance	66:82	glucose tolerance	66:82	The effect of moderate consumption of non-nutritive sweeteners on glucose tolerance and body composition in rats.
28692807	1	28	theme	body	136:139	arg1	composition					141:151	body composition	136:151	body composition	136:151	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28692807	3	29	theme	percent	393:399	arg1	fat					406:408	percent body fat	393:408	percent body fat	393:408	NNS treatment had no effect on weight gain or percent body fat.
28692807	1	30	dep	sweeteners	202:211	arg1	aspartame					220:228	aspartame	220:228	aspartame	220:228	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28692807	1	30	dep	sweeteners	202:211	arg1	sweeteners					202:211	non-nutritive sweeteners	188:211	non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water	188:260	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28692807	1	30	dep	sweeteners	202:211	arg1	sucralose					233:241	sucralose	233:241	sucralose	233:241	Glucose tolerance and body composition were determined in male rats given non-nutritive sweeteners (NNS) (aspartame or sucralose) in drinking water.
28926734	5	0	theme	central	814:820	arg1	design					846:851	A central composite face centered design	812:851	A central composite face centered design	812:851	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	5	0	theme	central	814:820	arg1	methodology					882:892	response surface methodology	865:892	response surface methodology for deriving the design space by Monte Carlo simulations	865:949	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	1	1	dep	product	323:329	arg1	sulfoxide					347:355	levomepromazine sulfoxide	331:355	the oxidation product levomepromazine sulfoxide in levomepromazine	309:374	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	2	2	theme	target	406:411	arg1	profile					413:419	The analytical target profile	391:419	The analytical target profile	391:419	The analytical target profile was defined that the method should be able to quantify 0.1% of both impurities with a precision of ≤10%.
28926734	1	3	theme	simultaneous	257:268	arg1	determination					270:282	the simultaneous determination	253:282	the simultaneous determination of dextromepromazine	253:303	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	5	4	theme	composite	822:830	arg1	design					846:851	A central composite face centered design	812:851	A central composite face centered design	812:851	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	5	4	theme	composite	822:830	arg1	methodology					882:892	response surface methodology	865:892	response surface methodology for deriving the design space by Monte Carlo simulations	865:949	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	6	5	theme	working	965:971	arg1	point					973:977	The selected working point	952:977	The selected working point	952:977	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	5	theme	working	965:971	arg1	buffer					1003:1008	a 100mM citric acid buffer	983:1008	a 100mM citric acid buffer	983:1008	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	2	6	theme	analytical	395:404	arg1	profile					413:419	The analytical target profile	391:419	The analytical target profile	391:419	The analytical target profile was defined that the method should be able to quantify 0.1% of both impurities with a precision of ≤10%.
28926734	4	7	theme	cyclodextrin	616:627	arg1	concentration					629:641	cyclodextrin concentration	616:641	cyclodextrin concentration	616:641	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	7	theme	cyclodextrin	616:627	arg1	parameters					605:614	The critical process parameters	584:614	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature	584:693	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	6	8	theme	selected	956:963	arg1	point					973:977	The selected working point	952:977	The selected working point	952:977	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	8	theme	selected	956:963	arg1	buffer					1003:1008	a 100mM citric acid buffer	983:1008	a 100mM citric acid buffer	983:1008	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	7	9	theme	Plackett-Burman	1148:1162	arg1	design					1164:1169	a Plackett-Burman design	1146:1169	a Plackett-Burman design	1146:1169	Robustness was estimated using a Plackett-Burman design.
28926734	9	10	theme	injection	1477:1485	arg1	solution					1487:1494	a commercial injection solution	1464:1494	a commercial injection solution	1464:1494	The method was applied to the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution.
28926734	4	11	theme	buffer	644:649	arg1	pH					651:652	buffer pH	644:652	buffer pH	644:652	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	11	theme	buffer	644:649	arg1	parameters					605:614	The critical process parameters	584:614	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature	584:693	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	12	dep	parameters	605:614	arg1	concentration					658:670	concentration	658:670	concentration as well as temperature	658:693	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	12	dep	parameters	605:614	arg1	concentration					629:641	cyclodextrin concentration	616:641	cyclodextrin concentration	616:641	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	12	dep	parameters	605:614	arg1	pH					651:652	buffer pH	644:652	buffer pH	644:652	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	12	dep	parameters	605:614	arg1	parameters					605:614	The critical process parameters	584:614	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature	584:693	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	1	13	from	sulfoxide	347:355	arg1	levomepromazine					360:374	levomepromazine	360:374	levomepromazine	360:374	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	4	14	theme	factorial	747:755	arg1	design					771:776	a fractional factorial resolution V+ design	734:776	a fractional factorial resolution V+ design for defining the knowledge space	734:809	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	9	15	theme	substance	1405:1413	arg1	purity					1381:1386	the purity	1377:1386	the purity of the reference substance of the European Pharmacopoeia	1377:1443	The method was applied to the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution.
28926734	1	16	theme	dextromepromazine	287:303	arg1	determination					270:282	the simultaneous determination	253:282	the simultaneous determination of dextromepromazine	253:303	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	4	17	theme	fractional	736:745	arg1	design					771:776	a fractional factorial resolution V+ design	734:776	a fractional factorial resolution V+ design for defining the knowledge space	734:809	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	8	18	theme	concentration	1226:1238	arg1	range					1240:1244	the relative concentration range	1213:1244	the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine	1213:1327	The method was subsequently validated in the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine.
28926734	5	19	theme	design	911:916	arg1	space					918:922	the design space	907:922	the design space	907:922	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	0	20	theme	dextromepromazine	96:112	arg1	determination					79:91	the determination	75:91	the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine	75:175	A quality by design-based approach to a capillary electrokinetic assay for the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine.
28926734	8	21	theme	%	1257:1257	arg1	range					1240:1244	the relative concentration range	1213:1244	the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine	1213:1327	The method was subsequently validated in the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine.
28926734	3	22	theme	chiral	567:572	arg1	selector					574:581	chiral selector	567:581	chiral selector	567:581	Hydroxypropyl-γ-cyclodextrin was used as chiral selector.
28926734	3	22	theme	chiral	567:572	arg1	Hydroxypropyl-γ-cyclodextrin					526:553	Hydroxypropyl-γ-cyclodextrin	526:553	Hydroxypropyl-γ-cyclodextrin	526:553	Hydroxypropyl-γ-cyclodextrin was used as chiral selector.
28926734	4	23	theme	applied	699:705	arg1	voltage					707:713	applied voltage	699:713	applied voltage	699:713	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	9	24	from	determination	1360:1372	arg1	solution					1487:1494	a commercial injection solution	1464:1494	a commercial injection solution	1464:1494	The method was applied to the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution.
28926734	4	25	theme	process	597:603	arg1	temperature					683:693	temperature	683:693	concentration as well as temperature	658:693	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	25	theme	process	597:603	arg1	concentration					658:670	concentration	658:670	concentration as well as temperature	658:693	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	25	theme	process	597:603	arg1	concentration					629:641	cyclodextrin concentration	616:641	cyclodextrin concentration	616:641	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	25	theme	process	597:603	arg1	pH					651:652	buffer pH	644:652	buffer pH	644:652	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	25	theme	process	597:603	arg1	parameters					605:614	The critical process parameters	584:614	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature	584:693	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	6	26	theme	25kV	1109:1112	arg1	hydroxypropyl-γ-cyclodextrin					1040:1067	3.6mg/mL hydroxypropyl-γ-cyclodextrin	1031:1067	3.6mg/mL hydroxypropyl-γ-cyclodextrin	1031:1067	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	26	theme	25kV	1109:1112	arg1	temperature					1072:1082	a temperature	1070:1082	a temperature of 15°C	1070:1090	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	26	theme	25kV	1109:1112	arg1	voltage					1098:1104	a voltage	1096:1104	a voltage of 25kV	1096:1112	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	9	27	theme	purity	1381:1386	arg1	determination					1360:1372	the determination	1356:1372	the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution	1356:1494	The method was applied to the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution.
28926734	4	28	theme	critical	588:595	arg1	temperature					683:693	temperature	683:693	concentration as well as temperature	658:693	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	28	theme	critical	588:595	arg1	concentration					658:670	concentration	658:670	concentration as well as temperature	658:693	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	28	theme	critical	588:595	arg1	concentration					629:641	cyclodextrin concentration	616:641	cyclodextrin concentration	616:641	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	28	theme	critical	588:595	arg1	pH					651:652	buffer pH	644:652	buffer pH	644:652	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	4	28	theme	critical	588:595	arg1	parameters					605:614	The critical process parameters	584:614	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature	584:693	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	6	29	contain	containing	1020:1029	arg2	temperature					1072:1082	a temperature	1070:1082	a temperature of 15°C	1070:1090	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	29	contain	containing	1020:1029	arg1	point					973:977	The selected working point	952:977	The selected working point	952:977	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	29	contain	containing	1020:1029	arg1	buffer					1003:1008	a 100mM citric acid buffer	983:1008	a 100mM citric acid buffer	983:1008	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	29	contain	containing	1020:1029	arg1	pH					1011:1012	pH 2.85	1011:1017	pH 2.85	1011:1017	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	29	contain	containing	1020:1029	arg2	hydroxypropyl-γ-cyclodextrin					1040:1067	3.6mg/mL hydroxypropyl-γ-cyclodextrin	1031:1067	3.6mg/mL hydroxypropyl-γ-cyclodextrin	1031:1067	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	29	contain	containing	1020:1029	arg2	voltage					1098:1104	a voltage	1096:1104	a voltage of 25kV	1096:1112	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	8	30	contain	containing	1292:1301	arg2	levomepromazine					1313:1327	0.25mg/mL levomepromazine	1303:1327	0.25mg/mL levomepromazine	1303:1327	The method was subsequently validated in the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine.
28926734	8	30	contain	containing	1292:1301	arg1	solution					1283:1290	a solution	1281:1290	a solution containing 0.25mg/mL levomepromazine	1281:1327	The method was subsequently validated in the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine.
28926734	1	31	theme	design	197:202	arg1	electrophoresis					226:240	a capillary electrophoresis	214:240	a capillary electrophoresis method	214:247	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	1	31	theme	design	197:202	arg1	approach					204:211	design approach	197:211	design approach	197:211	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	0	32	theme	design-based	13:24	arg1	approach					26:33	design-based approach	13:33	design-based approach to a capillary electrokinetic assay for the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine	13:175	A quality by design-based approach to a capillary electrokinetic assay for the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine.
28926734	8	33	theme	relative	1217:1224	arg1	range					1240:1244	the relative concentration range	1213:1244	the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine	1213:1327	The method was subsequently validated in the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine.
28926734	0	34	theme	sulfoxide	134:142	arg1	determination					79:91	the determination	75:91	the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine	75:175	A quality by design-based approach to a capillary electrokinetic assay for the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine.
28926734	8	35	theme	%	1252:1252	arg1	%					1257:1257	0.1%-1.0%	1249:1257	0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine	1249:1327	The method was subsequently validated in the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine.
28926734	8	35	theme	%	1252:1252	arg1	impurities					1266:1275	the impurities	1262:1275	the impurities for a solution containing 0.25mg/mL levomepromazine	1262:1327	The method was subsequently validated in the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine.
28926734	8	36	theme	0.25mg/mL	1303:1311	arg1	levomepromazine					1313:1327	0.25mg/mL levomepromazine	1303:1327	0.25mg/mL levomepromazine	1303:1327	The method was subsequently validated in the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine.
28926734	5	37	theme	surface	874:880	arg1	design					846:851	A central composite face centered design	812:851	A central composite face centered design	812:851	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	5	37	theme	surface	874:880	arg1	methodology					882:892	response surface methodology	865:892	response surface methodology for deriving the design space by Monte Carlo simulations	865:949	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	1	38	theme	oxidation	313:321	arg1	product					323:329	the oxidation product	309:329	the oxidation product levomepromazine sulfoxide in levomepromazine	309:374	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	0	39	theme	levomepromazine	118:132	arg1	sulfoxide					134:142	levomepromazine sulfoxide	118:142	levomepromazine sulfoxide	118:142	A quality by design-based approach to a capillary electrokinetic assay for the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine.
28926734	4	40	theme	knowledge	795:803	arg1	space					805:809	the knowledge space	791:809	the knowledge space	791:809	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	5	41	theme	face	832:835	arg1	design					846:851	A central composite face centered design	812:851	A central composite face centered design	812:851	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	5	41	theme	face	832:835	arg1	methodology					882:892	response surface methodology	865:892	response surface methodology for deriving the design space by Monte Carlo simulations	865:949	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	1	42	dep	developed	380:388	arg1	Using					178:182	Using	178:182	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine	178:303	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	5	43	theme	Monte	927:931	arg1	simulations					939:949	Monte Carlo simulations	927:949	Monte Carlo simulations	927:949	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	5	44	theme	centered	837:844	arg1	design					846:851	A central composite face centered design	812:851	A central composite face centered design	812:851	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	5	44	theme	centered	837:844	arg1	methodology					882:892	response surface methodology	865:892	response surface methodology for deriving the design space by Monte Carlo simulations	865:949	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	1	45	theme	levomepromazine	331:345	arg1	sulfoxide					347:355	levomepromazine sulfoxide	331:355	the oxidation product levomepromazine sulfoxide in levomepromazine	309:374	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	6	46	theme	3.6mg/mL	1031:1038	arg1	hydroxypropyl-γ-cyclodextrin					1040:1067	3.6mg/mL hydroxypropyl-γ-cyclodextrin	1031:1067	3.6mg/mL hydroxypropyl-γ-cyclodextrin	1031:1067	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	1	47	dep	electrophoresis	226:240	arg1	method					242:247	method	242:247	a capillary electrophoresis method	214:247	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	5	48	theme	response	865:872	arg1	design					846:851	A central composite face centered design	812:851	A central composite face centered design	812:851	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	5	48	theme	response	865:872	arg1	methodology					882:892	response surface methodology	865:892	response surface methodology for deriving the design space by Monte Carlo simulations	865:949	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	1	49	theme	capillary	216:224	arg1	electrophoresis					226:240	a capillary electrophoresis	214:240	a capillary electrophoresis method	214:247	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	1	49	theme	capillary	216:224	arg1	approach					204:211	design approach	197:211	design approach	197:211	Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed.
28926734	0	50	theme	capillary	40:48	arg1	assay					65:69	a capillary electrokinetic assay	38:69	a capillary electrokinetic assay for the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine	38:175	A quality by design-based approach to a capillary electrokinetic assay for the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine.
28926734	6	51	theme	acid	998:1001	arg1	pH					1011:1012	pH 2.85	1011:1017	pH 2.85	1011:1017	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	51	theme	acid	998:1001	arg1	point					973:977	The selected working point	952:977	The selected working point	952:977	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	51	theme	acid	998:1001	arg1	buffer					1003:1008	a 100mM citric acid buffer	983:1008	a 100mM citric acid buffer	983:1008	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	0	52	theme	levomepromazine	161:175	arg1	impurities					147:156	impurities	147:156	impurities of levomepromazine	147:175	A quality by design-based approach to a capillary electrokinetic assay for the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine.
28926734	0	53	theme	electrokinetic	50:63	arg1	assay					65:69	a capillary electrokinetic assay	38:69	a capillary electrokinetic assay for the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine	38:175	A quality by design-based approach to a capillary electrokinetic assay for the determination of dextromepromazine and levomepromazine sulfoxide as impurities of levomepromazine.
28926734	9	54	theme	commercial	1466:1475	arg1	solution					1487:1494	a commercial injection solution	1464:1494	a commercial injection solution	1464:1494	The method was applied to the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution.
28926734	4	55	theme	V+	768:769	arg1	design					771:776	a fractional factorial resolution V+ design	734:776	a fractional factorial resolution V+ design for defining the knowledge space	734:809	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	9	56	from	drug	1456:1459	arg1	solution					1487:1494	a commercial injection solution	1464:1494	a commercial injection solution	1464:1494	The method was applied to the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution.
28926734	6	57	theme	100mM	985:989	arg1	pH					1011:1012	pH 2.85	1011:1017	pH 2.85	1011:1017	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	57	theme	100mM	985:989	arg1	point					973:977	The selected working point	952:977	The selected working point	952:977	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	57	theme	100mM	985:989	arg1	buffer					1003:1008	a 100mM citric acid buffer	983:1008	a 100mM citric acid buffer	983:1008	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	4	58	theme	resolution	757:766	arg1	design					771:776	a fractional factorial resolution V+ design	734:776	a fractional factorial resolution V+ design for defining the knowledge space	734:809	The critical process parameters cyclodextrin concentration, buffer pH and concentration as well as temperature and applied voltage were studied using a fractional factorial resolution V+ design for defining the knowledge space.
28926734	9	59	theme	European	1422:1429	arg1	Pharmacopoeia					1431:1443	the European Pharmacopoeia	1418:1443	the European Pharmacopoeia	1418:1443	The method was applied to the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution.
28926734	5	60	used	used	857:860	arg2	methodology					882:892	response surface methodology	865:892	response surface methodology for deriving the design space by Monte Carlo simulations	865:949	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	5	60	used	used	857:860	arg2	design					846:851	A central composite face centered design	812:851	A central composite face centered design	812:851	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	9	61	theme	drug	1456:1459	arg1	determination					1360:1372	the determination	1356:1372	the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution	1356:1494	The method was applied to the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution.
28926734	9	62	from	solution	1487:1494	arg1	determination					1360:1372	the determination	1356:1372	the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution	1356:1494	The method was applied to the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution.
28926734	9	63	theme	reference	1395:1403	arg1	substance					1405:1413	the reference substance	1391:1413	the reference substance	1391:1413	The method was applied to the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution.
28926734	9	64	theme	Pharmacopoeia	1431:1443	arg1	purity					1381:1386	the purity	1377:1386	the purity of the reference substance of the European Pharmacopoeia	1377:1443	The method was applied to the determination of the purity of the reference substance of the European Pharmacopoeia and of the drug in a commercial injection solution.
28926734	2	65	theme	%	523:523	arg1	precision					507:515	a precision	505:515	a precision of ≤10%	505:523	The analytical target profile was defined that the method should be able to quantify 0.1% of both impurities with a precision of ≤10%.
28926734	6	66	theme	15°C	1087:1090	arg1	hydroxypropyl-γ-cyclodextrin					1040:1067	3.6mg/mL hydroxypropyl-γ-cyclodextrin	1031:1067	3.6mg/mL hydroxypropyl-γ-cyclodextrin	1031:1067	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	66	theme	15°C	1087:1090	arg1	temperature					1072:1082	a temperature	1070:1082	a temperature of 15°C	1070:1090	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	66	theme	15°C	1087:1090	arg1	voltage					1098:1104	a voltage	1096:1104	a voltage of 25kV	1096:1112	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	3	67	used	used	559:562	arg2	Hydroxypropyl-γ-cyclodextrin					526:553	Hydroxypropyl-γ-cyclodextrin	526:553	Hydroxypropyl-γ-cyclodextrin	526:553	Hydroxypropyl-γ-cyclodextrin was used as chiral selector.
28926734	3	67	used	used	559:562	arg2	selector					574:581	chiral selector	567:581	chiral selector	567:581	Hydroxypropyl-γ-cyclodextrin was used as chiral selector.
28926734	5	68	theme	Carlo	933:937	arg1	simulations					939:949	Monte Carlo simulations	927:949	Monte Carlo simulations	927:949	A central composite face centered design was used as response surface methodology for deriving the design space by Monte Carlo simulations.
28926734	8	69	theme	impurities	1266:1275	arg1	%					1257:1257	0.1%-1.0%	1249:1257	0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine	1249:1327	The method was subsequently validated in the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine.
28926734	8	69	theme	impurities	1266:1275	arg1	impurities					1266:1275	the impurities	1262:1275	the impurities for a solution containing 0.25mg/mL levomepromazine	1262:1327	The method was subsequently validated in the relative concentration range of 0.1%-1.0% of the impurities for a solution containing 0.25mg/mL levomepromazine.
28926734	2	70	theme	impurities	489:498	arg1	%					479:479	0.1%	476:479	0.1% of both impurities	476:498	The analytical target profile was defined that the method should be able to quantify 0.1% of both impurities with a precision of ≤10%.
28926734	2	70	theme	impurities	489:498	arg1	impurities					489:498	both impurities	484:498	both impurities	484:498	The analytical target profile was defined that the method should be able to quantify 0.1% of both impurities with a precision of ≤10%.
28926734	6	71	theme	citric	991:996	arg1	pH					1011:1012	pH 2.85	1011:1017	pH 2.85	1011:1017	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	71	theme	citric	991:996	arg1	point					973:977	The selected working point	952:977	The selected working point	952:977	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
28926734	6	71	theme	citric	991:996	arg1	buffer					1003:1008	a 100mM citric acid buffer	983:1008	a 100mM citric acid buffer	983:1008	The selected working point was a 100mM citric acid buffer, pH 2.85, containing 3.6mg/mL hydroxypropyl-γ-cyclodextrin, a temperature of 15°C and a voltage of 25kV.
27762500	3	0	theme	homogeneous	679:689	arg1	heating					691:697	homogeneous heating	679:697	homogeneous heating of the sample in a microwave reactor	679:734	In the second step, the porous structure of the foaming was chemically stabilized by radical polymerization induced by homogeneous heating of the sample in a microwave reactor.
27762500	1	1	theme	foaming	161:167	arg1	method					169:174	a new foaming method	155:174	a new foaming method	155:174	In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration.
27762500	9	2	theme	bioactivity	1445:1455	arg1	properties					1472:1481	the bioactivity and biological properties	1441:1481	the bioactivity and biological properties of chitosan scaffold	1441:1502	To improve the bioactivity and biological properties of chitosan scaffold, a biomineralization process was used.
27762500	0	3	theme	mechanical	86:95	arg1	properties					97:106	mechanical properties	86:106	mechanical properties	86:106	Microwave-induced porosity and bioactivation of chitosan-PEGDA scaffolds: morphology, mechanical properties and osteogenic differentiation.
27762500	10	4	theme	scaffold	1641:1648	arg1	effect					1613:1618	the effect	1609:1618	the effect of biomineralization scaffold on human mesenchymal stem cells behaviour	1609:1690	Biological characterization was carried out with the aim to prove the effect of biomineralization scaffold on human mesenchymal stem cells behaviour.
27762500	2	5	theme	first	356:360	arg1	step					362:365	the first step	352:365	the first step of the process	352:380	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	8	6	theme	degradation	1383:1393	arg1	test					1395:1398	degradation test	1383:1398	degradation test	1383:1398	The stability of materials was evaluated in vitro by degradation test in phosphate-buffered saline.
27762500	0	7	theme	osteogenic	112:121	arg1	differentiation					123:137	osteogenic differentiation	112:137	osteogenic differentiation	112:137	Microwave-induced porosity and bioactivation of chitosan-PEGDA scaffolds: morphology, mechanical properties and osteogenic differentiation.
27762500	6	8	theme	kinetic	1157:1163	arg1	parameters					1165:1174	kinetic parameters	1157:1174	kinetic parameters (i.e. time curing, microwave power)	1157:1210	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	6	8	theme	kinetic	1157:1163	arg1	curing					1187:1192	i.e. time curing	1177:1192	i.e. time curing	1177:1192	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	8	9	theme	materials	1347:1355	arg1	stability					1334:1342	The stability	1330:1342	The stability of materials	1330:1355	The stability of materials was evaluated in vitro by degradation test in phosphate-buffered saline.
27762500	9	10	theme	scaffold	1495:1502	arg1	properties					1472:1481	the bioactivity and biological properties	1441:1481	the bioactivity and biological properties of chitosan scaffold	1441:1502	To improve the bioactivity and biological properties of chitosan scaffold, a biomineralization process was used.
27762500	6	11	dep	evaluated	1023:1031	arg1	before					1084:1089	before	1084:1089	before	1084:1089	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	6	11	dep	evaluated	1023:1031	arg1	curing					1101:1106	curing	1101:1106	curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power)	1101:1210	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	2	12	theme	Chitosan	494:501	arg1	blend					485:489	a homogeneous blend	471:489	a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions	471:557	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	6	13	theme	reaction	1129:1136	arg1	yield					1138:1142	reaction yield	1129:1142	reaction yield	1129:1142	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	7	14	theme	Absorption	1213:1222	arg1	capacity					1224:1231	Absorption capacity	1213:1231	Absorption capacity	1213:1231	Absorption capacity, elastic modulus, porosity and morphology of the porous structure were measured for each sample.
27762500	3	15	theme	sample	706:711	arg1	heating					691:697	homogeneous heating	679:697	homogeneous heating of the sample in a microwave reactor	679:734	In the second step, the porous structure of the foaming was chemically stabilized by radical polymerization induced by homogeneous heating of the sample in a microwave reactor.
27762500	6	16	theme	attenuated	1045:1054	arg1	analysis					1074:1081	infrared attenuated total reflectance analysis	1036:1081	infrared attenuated total reflectance analysis	1036:1081	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	5	17	theme	composition	926:936	arg1	influence					909:917	the influence	905:917	the influence of the composition on the final properties of the material	905:976	Chitosan and PEGDA were mixed in different blends to investigate the influence of the composition on the final properties of the material.
27762500	7	18	theme	porous	1282:1287	arg1	structure					1289:1297	the porous structure	1278:1297	the porous structure	1278:1297	Absorption capacity, elastic modulus, porosity and morphology of the porous structure were measured for each sample.
27762500	2	19	theme	stable	385:390	arg1	foaming					401:407	a stable physical foaming	383:407	a stable physical foaming	383:407	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	2	20	theme	blend	485:489	arg1	agent					462:466	blowing agent	454:466	blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions	454:557	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	10	21	theme	mesenchymal	1659:1669	arg1	cells					1676:1680	human mesenchymal stem cells behaviour	1653:1690	human mesenchymal stem cells behaviour	1653:1690	Biological characterization was carried out with the aim to prove the effect of biomineralization scaffold on human mesenchymal stem cells behaviour.
27762500	0	22	theme	chitosan-PEGDA	48:61	arg1	scaffolds					63:71	chitosan-PEGDA scaffolds	48:71	chitosan-PEGDA scaffolds	48:71	Microwave-induced porosity and bioactivation of chitosan-PEGDA scaffolds: morphology, mechanical properties and osteogenic differentiation.
27762500	9	23	theme	biomineralization	1507:1523	arg1	process					1525:1531	a biomineralization process	1505:1531	a biomineralization process	1505:1531	To improve the bioactivity and biological properties of chitosan scaffold, a biomineralization process was used.
27762500	3	24	from	heating	691:697	arg1	reactor					728:734	a microwave reactor	716:734	a microwave reactor	716:734	In the second step, the porous structure of the foaming was chemically stabilized by radical polymerization induced by homogeneous heating of the sample in a microwave reactor.
27762500	6	25	theme	microwave	1195:1203	arg1	power					1205:1209	microwave power	1195:1209	microwave power	1195:1209	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	3	26	theme	microwave	718:726	arg1	reactor					728:734	a microwave reactor	716:734	a microwave reactor	716:734	In the second step, the porous structure of the foaming was chemically stabilized by radical polymerization induced by homogeneous heating of the sample in a microwave reactor.
27762500	6	27	theme	time	1182:1185	arg1	parameters					1165:1174	kinetic parameters	1157:1174	kinetic parameters (i.e. time curing, microwave power)	1157:1210	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	6	27	theme	time	1182:1185	arg1	curing					1187:1192	i.e. time curing	1177:1192	i.e. time curing	1177:1192	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	4	28	used	used	810:813	arg2	dihydrochloride					790:804	2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride	751:804	2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride	751:804	In this step, 2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride was used as thermoinitiator (TI).
27762500	4	28	used	used	810:813	arg2	thermoinitiator					818:832	thermoinitiator	818:832	thermoinitiator (TI)	818:837	In this step, 2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride was used as thermoinitiator (TI).
27762500	0	29	theme	Microwave-induced	0:16	arg1	porosity					18:25	Microwave-induced porosity	0:25	Microwave-induced porosity	0:25	Microwave-induced porosity and bioactivation of chitosan-PEGDA scaffolds: morphology, mechanical properties and osteogenic differentiation.
27762500	1	30	theme	surface	277:283	arg1	bioactivation					285:297	a surface bioactivation	275:297	a surface bioactivation	275:297	In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration.
27762500	2	31	dep	surfactant	429:438	arg1	Pluronic					441:448	Pluronic	441:448	Pluronic	441:448	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	10	32	theme	human	1653:1657	arg1	cells					1676:1680	human mesenchymal stem cells behaviour	1653:1690	human mesenchymal stem cells behaviour	1653:1690	Biological characterization was carried out with the aim to prove the effect of biomineralization scaffold on human mesenchymal stem cells behaviour.
27762500	2	33	theme	process	374:380	arg1	step					362:365	the first step	352:365	the first step of the process	352:380	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	4	34	theme	2-imidazolin-2yl	765:780	arg1	thermoinitiator					818:832	thermoinitiator	818:832	thermoinitiator (TI)	818:837	In this step, 2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride was used as thermoinitiator (TI).
27762500	4	34	theme	2-imidazolin-2yl	765:780	arg1	dihydrochloride					790:804	2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride	751:804	2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride	751:804	In this step, 2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride was used as thermoinitiator (TI).
27762500	10	35	dep	cells	1676:1680	arg1	behaviour					1682:1690	behaviour	1682:1690	behaviour	1682:1690	Biological characterization was carried out with the aim to prove the effect of biomineralization scaffold on human mesenchymal stem cells behaviour.
27762500	4	36	theme	propane	782:788	arg1	thermoinitiator					818:832	thermoinitiator	818:832	thermoinitiator (TI)	818:837	In this step, 2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride was used as thermoinitiator (TI).
27762500	4	36	theme	propane	782:788	arg1	dihydrochloride					790:804	2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride	751:804	2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride	751:804	In this step, 2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride was used as thermoinitiator (TI).
27762500	10	37	theme	Biological	1543:1552	arg1	characterization					1554:1569	Biological characterization	1543:1569	Biological characterization	1543:1569	Biological characterization was carried out with the aim to prove the effect of biomineralization scaffold on human mesenchymal stem cells behaviour.
27762500	2	38	theme	diacrylate	527:536	arg1	solutions					549:557	diacrylate (PEGDA700) solutions	527:557	diacrylate (PEGDA700) solutions	527:557	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	3	39	theme	foaming	608:614	arg1	structure					591:599	the porous structure	580:599	the porous structure of the foaming	580:614	In the second step, the porous structure of the foaming was chemically stabilized by radical polymerization induced by homogeneous heating of the sample in a microwave reactor.
27762500	6	40	theme	infrared	1036:1043	arg1	analysis					1074:1081	infrared attenuated total reflectance analysis	1036:1081	infrared attenuated total reflectance analysis	1036:1081	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	9	41	theme	chitosan	1486:1493	arg1	scaffold					1495:1502	chitosan scaffold	1486:1502	chitosan scaffold	1486:1502	To improve the bioactivity and biological properties of chitosan scaffold, a biomineralization process was used.
27762500	9	42	theme	biological	1461:1470	arg1	properties					1472:1481	the bioactivity and biological properties	1441:1481	the bioactivity and biological properties of chitosan scaffold	1441:1502	To improve the bioactivity and biological properties of chitosan scaffold, a biomineralization process was used.
27762500	2	43	theme	blowing	454:460	arg1	agent					462:466	blowing agent	454:466	blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions	454:557	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	2	44	theme	glycol	520:525	arg1	blend					485:489	a homogeneous blend	471:489	a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions	471:557	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	10	45	theme	biomineralization	1623:1639	arg1	scaffold					1641:1648	biomineralization scaffold	1623:1648	biomineralization scaffold	1623:1648	Biological characterization was carried out with the aim to prove the effect of biomineralization scaffold on human mesenchymal stem cells behaviour.
27762500	3	46	theme	second	567:572	arg1	step					574:577	the second step	563:577	the second step	563:577	In the second step, the porous structure of the foaming was chemically stabilized by radical polymerization induced by homogeneous heating of the sample in a microwave reactor.
27762500	1	47	theme	physical	186:193	arg1	foaming					195:201	physical foaming	186:201	physical foaming combined with microwave-induced curing	186:240	In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration.
27762500	7	48	theme	elastic	1234:1240	arg1	modulus					1242:1248	elastic modulus	1234:1248	elastic modulus	1234:1248	Absorption capacity, elastic modulus, porosity and morphology of the porous structure were measured for each sample.
27762500	6	49	dep	curing	1187:1192	arg1	power					1205:1209	microwave power	1195:1209	microwave power	1195:1209	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	2	50	theme	polyethylene	507:518	arg1	glycol					520:525	polyethylene glycol	507:525	polyethylene glycol	507:525	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	5	51	theme	material	969:976	arg1	properties					951:960	the final properties	941:960	the final properties of the material	941:976	Chitosan and PEGDA were mixed in different blends to investigate the influence of the composition on the final properties of the material.
27762500	1	52	theme	microwave-induced	217:233	arg1	curing					235:240	microwave-induced curing	217:240	microwave-induced curing	217:240	In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration.
27762500	9	53	used	used	1537:1540	arg2	process					1525:1531	a biomineralization process	1505:1531	a biomineralization process	1505:1531	To improve the bioactivity and biological properties of chitosan scaffold, a biomineralization process was used.
27762500	6	54	theme	chemical	983:990	arg1	properties					992:1001	The chemical properties	979:1001	The chemical properties of each sample	979:1016	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	11	55	dep	&	1721:1721	arg1	Sons					1723:1726	Sons	1723:1726	Sons	1723:1726	Copyright © 2016 John Wiley & Sons, Ltd.
27762500	6	56	theme	i.e.	1177:1180	arg1	parameters					1165:1174	kinetic parameters	1157:1174	kinetic parameters (i.e. time curing, microwave power)	1157:1210	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	6	56	theme	i.e.	1177:1180	arg1	curing					1187:1192	i.e. time curing	1177:1192	i.e. time curing	1177:1192	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	0	57	theme	scaffolds	63:71	arg1	bioactivation					31:43	bioactivation	31:43	bioactivation	31:43	Microwave-induced porosity and bioactivation of chitosan-PEGDA scaffolds: morphology, mechanical properties and osteogenic differentiation.
27762500	0	57	theme	scaffolds	63:71	arg1	porosity					18:25	Microwave-induced porosity	0:25	Microwave-induced porosity	0:25	Microwave-induced porosity and bioactivation of chitosan-PEGDA scaffolds: morphology, mechanical properties and osteogenic differentiation.
27762500	1	58	theme	bone	323:326	arg1	regeneration					335:346	bone tissue regeneration	323:346	bone tissue regeneration	323:346	In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration.
27762500	6	59	theme	sample	1011:1016	arg1	properties					992:1001	The chemical properties	979:1001	The chemical properties of each sample	979:1016	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	8	60	theme	phosphate-buffered	1403:1420	arg1	saline					1422:1427	phosphate-buffered saline	1403:1427	phosphate-buffered saline	1403:1427	The stability of materials was evaluated in vitro by degradation test in phosphate-buffered saline.
27762500	10	61	from	effect	1613:1618	arg1	cells					1676:1680	human mesenchymal stem cells behaviour	1653:1690	human mesenchymal stem cells behaviour	1653:1690	Biological characterization was carried out with the aim to prove the effect of biomineralization scaffold on human mesenchymal stem cells behaviour.
27762500	10	62	theme	stem	1671:1674	arg1	cells					1676:1680	human mesenchymal stem cells behaviour	1653:1690	human mesenchymal stem cells behaviour	1653:1690	Biological characterization was carried out with the aim to prove the effect of biomineralization scaffold on human mesenchymal stem cells behaviour.
27762500	1	63	theme	tissue	328:333	arg1	regeneration					335:346	bone tissue regeneration	323:346	bone tissue regeneration	323:346	In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration.
27762500	3	64	theme	radical	645:651	arg1	polymerization					653:666	radical polymerization	645:666	radical polymerization induced by homogeneous heating of the sample in a microwave reactor	645:734	In the second step, the porous structure of the foaming was chemically stabilized by radical polymerization induced by homogeneous heating of the sample in a microwave reactor.
27762500	3	65	theme	porous	584:589	arg1	structure					591:599	the porous structure	580:599	the porous structure of the foaming	580:614	In the second step, the porous structure of the foaming was chemically stabilized by radical polymerization induced by homogeneous heating of the sample in a microwave reactor.
27762500	1	66	with	combination	258:268	arg1	bioactivation					285:297	a surface bioactivation	275:297	a surface bioactivation	275:297	In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration.
27762500	5	67	theme	different	873:881	arg1	blends					883:888	different blends	873:888	different blends	873:888	Chitosan and PEGDA were mixed in different blends to investigate the influence of the composition on the final properties of the material.
27762500	5	68	from	influence	909:917	arg1	properties					951:960	the final properties	941:960	the final properties of the material	941:976	Chitosan and PEGDA were mixed in different blends to investigate the influence of the composition on the final properties of the material.
27762500	5	69	theme	final	945:949	arg1	properties					951:960	the final properties	941:960	the final properties of the material	941:976	Chitosan and PEGDA were mixed in different blends to investigate the influence of the composition on the final properties of the material.
27762500	2	70	dep	Chitosan	494:501	arg1	solutions					549:557	diacrylate (PEGDA700) solutions	527:557	diacrylate (PEGDA700) solutions	527:557	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	6	71	theme	total	1056:1060	arg1	analysis					1074:1081	infrared attenuated total reflectance analysis	1036:1081	infrared attenuated total reflectance analysis	1036:1081	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	4	72	theme	2,2-azobis[2-	751:763	arg1	thermoinitiator					818:832	thermoinitiator	818:832	thermoinitiator (TI)	818:837	In this step, 2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride was used as thermoinitiator (TI).
27762500	4	72	theme	2,2-azobis[2-	751:763	arg1	dihydrochloride					790:804	2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride	751:804	2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride	751:804	In this step, 2,2-azobis[2-(2-imidazolin-2yl)propane]dihydrochloride was used as thermoinitiator (TI).
27762500	2	73	theme	homogeneous	473:483	arg1	blend					485:489	a homogeneous blend	471:489	a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions	471:557	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	2	74	theme	physical	392:399	arg1	foaming					401:407	a stable physical foaming	383:407	a stable physical foaming	383:407	In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowing agent of a homogeneous blend of Chitosan and polyethylene glycol diacrylate (PEGDA700) solutions.
27762500	0	75	dep	porosity	18:25	arg1	morphology					74:83	morphology	74:83	morphology	74:83	Microwave-induced porosity and bioactivation of chitosan-PEGDA scaffolds: morphology, mechanical properties and osteogenic differentiation.
27762500	0	75	dep	porosity	18:25	arg1	properties					97:106	mechanical properties	86:106	mechanical properties	86:106	Microwave-induced porosity and bioactivation of chitosan-PEGDA scaffolds: morphology, mechanical properties and osteogenic differentiation.
27762500	0	75	dep	porosity	18:25	arg1	differentiation					123:137	osteogenic differentiation	112:137	osteogenic differentiation	112:137	Microwave-induced porosity and bioactivation of chitosan-PEGDA scaffolds: morphology, mechanical properties and osteogenic differentiation.
27762500	6	76	theme	reflectance	1062:1072	arg1	analysis					1074:1081	infrared attenuated total reflectance analysis	1036:1081	infrared attenuated total reflectance analysis	1036:1081	The chemical properties of each sample were evaluated by infrared attenuated total reflectance analysis, before and after curing in order to maximize reaction yield and optimize kinetic parameters (i.e. time curing, microwave power).
27762500	7	77	theme	structure	1289:1297	arg1	morphology					1264:1273	morphology	1264:1273	morphology	1264:1273	Absorption capacity, elastic modulus, porosity and morphology of the porous structure were measured for each sample.
27762500	7	77	theme	structure	1289:1297	arg1	porosity					1251:1258	porosity	1251:1258	porosity	1251:1258	Absorption capacity, elastic modulus, porosity and morphology of the porous structure were measured for each sample.
27762500	7	77	theme	structure	1289:1297	arg1	capacity					1224:1231	Absorption capacity	1213:1231	Absorption capacity	1213:1231	Absorption capacity, elastic modulus, porosity and morphology of the porous structure were measured for each sample.
27762500	7	77	theme	structure	1289:1297	arg1	modulus					1242:1248	elastic modulus	1234:1248	elastic modulus	1234:1248	Absorption capacity, elastic modulus, porosity and morphology of the porous structure were measured for each sample.
27762500	1	78	theme	new	157:159	arg1	method					169:174	a new foaming method	155:174	a new foaming method	155:174	In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration.
25709014	7	0	theme	SF/CS/Nano	1011:1020	arg1	candidate					1061:1069	a potential candidate	1049:1069	a potential candidate to be used for tissue engineering	1049:1103	Thus, we suggest that SF/CS/Nano ZrO2 composite scaffold is a potential candidate to be used for tissue engineering.
25709014	7	0	theme	SF/CS/Nano	1011:1020	arg1	scaffold					1037:1044	SF/CS/Nano ZrO2 composite scaffold	1011:1044	SF/CS/Nano ZrO2 composite scaffold	1011:1044	Thus, we suggest that SF/CS/Nano ZrO2 composite scaffold is a potential candidate to be used for tissue engineering.
25709014	5	1	attach	presence	670:677	arg2	zirconia					682:689	zirconia	682:689	zirconia	682:689	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	5	1	attach	presence	670:677	arg1	scaffold					714:721	the SF/CS/Nano ZrO2 scaffold	694:721	the SF/CS/Nano ZrO2 scaffold	694:721	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	0	2	theme	silk	18:21	arg1	ZrO2					45:48	silk fibroin-chitosan/Nano ZrO2	18:48	silk fibroin-chitosan/Nano ZrO2	18:48	Nano-composite of silk fibroin-chitosan/Nano ZrO2 for tissue engineering applications: fabrication and morphology.
25709014	5	3	theme	SF/CS/Nano	698:707	arg1	scaffold					714:721	the SF/CS/Nano ZrO2 scaffold	694:721	the SF/CS/Nano ZrO2 scaffold	694:721	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	7	4	theme	ZrO2	1022:1025	arg1	candidate					1061:1069	a potential candidate	1049:1069	a potential candidate to be used for tissue engineering	1049:1103	Thus, we suggest that SF/CS/Nano ZrO2 composite scaffold is a potential candidate to be used for tissue engineering.
25709014	7	4	theme	ZrO2	1022:1025	arg1	scaffold					1037:1044	SF/CS/Nano ZrO2 composite scaffold	1011:1044	SF/CS/Nano ZrO2 composite scaffold	1011:1044	Thus, we suggest that SF/CS/Nano ZrO2 composite scaffold is a potential candidate to be used for tissue engineering.
25709014	3	5	theme	SF/CS/Nano	470:479	arg1	scaffold					460:467	The composite scaffold	446:467	The composite scaffold (SF/CS/Nano ZrO2)	446:485	The composite scaffold (SF/CS/Nano ZrO2) was characterized by SEM, XRD, TGA, BET and FT-IR studies.
25709014	3	5	theme	SF/CS/Nano	470:479	arg1	ZrO2					481:484	SF/CS/Nano ZrO2	470:484	SF/CS/Nano ZrO2	470:484	The composite scaffold (SF/CS/Nano ZrO2) was characterized by SEM, XRD, TGA, BET and FT-IR studies.
25709014	7	6	theme	tissue	1086:1091	arg1	engineering					1093:1103	tissue engineering	1086:1103	tissue engineering	1086:1103	Thus, we suggest that SF/CS/Nano ZrO2 composite scaffold is a potential candidate to be used for tissue engineering.
25709014	1	7	contain	possessing	126:135	arg1	template					242:249	a template	240:249	a template for tissue engineering	240:272	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	1	7	contain	possessing	126:135	arg1	scaffold					117:124	A scaffold	115:124	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure	115:223	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	1	7	contain	possessing	126:135	arg2	features					153:160	certain desired features	137:160	certain desired features such as biodegradation, biocompatibility, and porous structure	137:223	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	1	7	contain	possessing	126:135	arg2	biocompatibility					186:201	biocompatibility	186:201	biocompatibility	186:201	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	1	7	contain	possessing	126:135	arg2	biodegradation					170:183	biodegradation	170:183	biodegradation	170:183	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	1	7	contain	possessing	126:135	arg2	structure					215:223	porous structure	208:223	porous structure	208:223	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	2	8	theme	silk	297:300	arg1	SF					311:312	SF	311:312	SF	311:312	In the present study, silk fibroin (SF), chitosan (CS) and zirconia (Nano ZrO2) were all combined using the freeze drying technique to fabricate a bio-composite scaffold.
25709014	2	8	theme	silk	297:300	arg1	fibroin					302:308	silk fibroin	297:308	silk fibroin (SF)	297:313	In the present study, silk fibroin (SF), chitosan (CS) and zirconia (Nano ZrO2) were all combined using the freeze drying technique to fabricate a bio-composite scaffold.
25709014	6	9	dep	Fibroblast	958:967	arg1	HGF					970:972	HGF	970:972	HGF	970:972	Cytocompatibility of the SF/CS/Nano ZrO2 scaffold, assessed by MTT assay, revealed non-toxicity to the Human Gingival Fibroblast (HGF, NCBI: C-131).
25709014	6	9	dep	Fibroblast	958:967	arg1	NCBI					975:978	NCBI	975:978	NCBI	975:978	Cytocompatibility of the SF/CS/Nano ZrO2 scaffold, assessed by MTT assay, revealed non-toxicity to the Human Gingival Fibroblast (HGF, NCBI: C-131).
25709014	4	10	theme	porous	582:587	arg1	nature					589:594	a porous nature	580:594	a porous nature with pore dimensions suitable for cell infiltration and colonization	580:663	The scaffold was found to possess a porous nature with pore dimensions suitable for cell infiltration and colonization.
25709014	2	11	theme	present	282:288	arg1	study					290:294	the present study	278:294	the present study	278:294	In the present study, silk fibroin (SF), chitosan (CS) and zirconia (Nano ZrO2) were all combined using the freeze drying technique to fabricate a bio-composite scaffold.
25709014	5	12	from	increase	733:740	arg1	strength					757:764	compressive strength	745:764	compressive strength	745:764	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	5	12	from	increase	733:740	arg1	capacity					783:790	water uptake capacity	770:790	water uptake capacity	770:790	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	6	13	theme	MTT	903:905	arg1	assay					907:911	MTT assay	903:911	MTT assay	903:911	Cytocompatibility of the SF/CS/Nano ZrO2 scaffold, assessed by MTT assay, revealed non-toxicity to the Human Gingival Fibroblast (HGF, NCBI: C-131).
25709014	0	14	theme	fibroin-chitosan/Nano	23:43	arg1	ZrO2					45:48	silk fibroin-chitosan/Nano ZrO2	18:48	silk fibroin-chitosan/Nano ZrO2	18:48	Nano-composite of silk fibroin-chitosan/Nano ZrO2 for tissue engineering applications: fabrication and morphology.
25709014	2	15	theme	Nano	344:347	arg1	zirconia					334:341	zirconia	334:341	zirconia (Nano ZrO2)	334:353	In the present study, silk fibroin (SF), chitosan (CS) and zirconia (Nano ZrO2) were all combined using the freeze drying technique to fabricate a bio-composite scaffold.
25709014	2	15	theme	Nano	344:347	arg1	ZrO2					349:352	Nano ZrO2	344:352	Nano ZrO2	344:352	In the present study, silk fibroin (SF), chitosan (CS) and zirconia (Nano ZrO2) were all combined using the freeze drying technique to fabricate a bio-composite scaffold.
25709014	2	16	theme	drying	390:395	arg1	technique					397:405	the freeze drying technique	379:405	the freeze drying technique	379:405	In the present study, silk fibroin (SF), chitosan (CS) and zirconia (Nano ZrO2) were all combined using the freeze drying technique to fabricate a bio-composite scaffold.
25709014	4	17	with	nature	589:594	arg1	dimensions					606:615	pore dimensions	601:615	pore dimensions suitable for cell infiltration and colonization	601:663	The scaffold was found to possess a porous nature with pore dimensions suitable for cell infiltration and colonization.
25709014	2	18	theme	freeze	383:388	arg1	technique					397:405	the freeze drying technique	379:405	the freeze drying technique	379:405	In the present study, silk fibroin (SF), chitosan (CS) and zirconia (Nano ZrO2) were all combined using the freeze drying technique to fabricate a bio-composite scaffold.
25709014	5	19	from	presence	670:677	arg1	scaffold					714:721	the SF/CS/Nano ZrO2 scaffold	694:721	the SF/CS/Nano ZrO2 scaffold	694:721	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	4	20	theme	suitable	617:624	arg1	dimensions					606:615	pore dimensions	601:615	pore dimensions suitable for cell infiltration and colonization	601:663	The scaffold was found to possess a porous nature with pore dimensions suitable for cell infiltration and colonization.
25709014	5	21	theme	ZrO2	709:712	arg1	scaffold					714:721	the SF/CS/Nano ZrO2 scaffold	694:721	the SF/CS/Nano ZrO2 scaffold	694:721	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	4	22	theme	cell	630:633	arg1	infiltration					635:646	cell infiltration	630:646	cell infiltration	630:646	The scaffold was found to possess a porous nature with pore dimensions suitable for cell infiltration and colonization.
25709014	4	23	contain	possess	572:578	arg2	nature					589:594	a porous nature	580:594	a porous nature with pore dimensions suitable for cell infiltration and colonization	580:663	The scaffold was found to possess a porous nature with pore dimensions suitable for cell infiltration and colonization.
25709014	4	23	contain	possess	572:578	arg1	scaffold					550:557	The scaffold	546:557	The scaffold	546:557	The scaffold was found to possess a porous nature with pore dimensions suitable for cell infiltration and colonization.
25709014	1	24	theme	porous	208:213	arg1	structure					215:223	porous structure	208:223	porous structure	208:223	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	5	25	theme	water	770:774	arg1	capacity					783:790	water uptake capacity	770:790	water uptake capacity	770:790	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	2	26	theme	bio-composite	422:434	arg1	scaffold					436:443	a bio-composite scaffold	420:443	a bio-composite scaffold	420:443	In the present study, silk fibroin (SF), chitosan (CS) and zirconia (Nano ZrO2) were all combined using the freeze drying technique to fabricate a bio-composite scaffold.
25709014	6	27	theme	scaffold	881:888	arg1	Cytocompatibility					840:856	Cytocompatibility	840:856	Cytocompatibility	840:856	Cytocompatibility of the SF/CS/Nano ZrO2 scaffold, assessed by MTT assay, revealed non-toxicity to the Human Gingival Fibroblast (HGF, NCBI: C-131).
25709014	0	28	theme	ZrO2	45:48	arg1	Nano-composite					0:13	Nano-composite	0:13	Nano-composite of silk fibroin-chitosan/Nano ZrO2 for tissue engineering applications: fabrication and morphology.	0:113	Nano-composite of silk fibroin-chitosan/Nano ZrO2 for tissue engineering applications: fabrication and morphology.
25709014	0	28	theme	ZrO2	45:48	arg1	ZrO2					45:48	silk fibroin-chitosan/Nano ZrO2	18:48	silk fibroin-chitosan/Nano ZrO2	18:48	Nano-composite of silk fibroin-chitosan/Nano ZrO2 for tissue engineering applications: fabrication and morphology.
25709014	3	29	theme	FT-IR	531:535	arg1	studies					537:543	FT-IR studies	531:543	FT-IR studies	531:543	The composite scaffold (SF/CS/Nano ZrO2) was characterized by SEM, XRD, TGA, BET and FT-IR studies.
25709014	5	30	theme	uptake	776:781	arg1	capacity					783:790	water uptake capacity	770:790	water uptake capacity	770:790	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	0	31	theme	engineering	61:71	arg1	applications					73:84	tissue engineering applications	54:84	tissue engineering applications	54:84	Nano-composite of silk fibroin-chitosan/Nano ZrO2 for tissue engineering applications: fabrication and morphology.
25709014	6	32	theme	ZrO2	876:879	arg1	scaffold					881:888	the SF/CS/Nano ZrO2 scaffold	861:888	the SF/CS/Nano ZrO2 scaffold	861:888	Cytocompatibility of the SF/CS/Nano ZrO2 scaffold, assessed by MTT assay, revealed non-toxicity to the Human Gingival Fibroblast (HGF, NCBI: C-131).
25709014	3	33	theme	composite	450:458	arg1	scaffold					460:467	The composite scaffold	446:467	The composite scaffold (SF/CS/Nano ZrO2)	446:485	The composite scaffold (SF/CS/Nano ZrO2) was characterized by SEM, XRD, TGA, BET and FT-IR studies.
25709014	3	33	theme	composite	450:458	arg1	ZrO2					481:484	SF/CS/Nano ZrO2	470:484	SF/CS/Nano ZrO2	470:484	The composite scaffold (SF/CS/Nano ZrO2) was characterized by SEM, XRD, TGA, BET and FT-IR studies.
25709014	0	34	theme	tissue	54:59	arg1	applications					73:84	tissue engineering applications	54:84	tissue engineering applications	54:84	Nano-composite of silk fibroin-chitosan/Nano ZrO2 for tissue engineering applications: fabrication and morphology.
25709014	6	35	theme	SF/CS/Nano	865:874	arg1	scaffold					881:888	the SF/CS/Nano ZrO2 scaffold	861:888	the SF/CS/Nano ZrO2 scaffold	861:888	Cytocompatibility of the SF/CS/Nano ZrO2 scaffold, assessed by MTT assay, revealed non-toxicity to the Human Gingival Fibroblast (HGF, NCBI: C-131).
25709014	1	36	theme	certain	137:143	arg1	biocompatibility					186:201	biocompatibility	186:201	biocompatibility	186:201	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	1	36	theme	certain	137:143	arg1	features					153:160	certain desired features	137:160	certain desired features such as biodegradation, biocompatibility, and porous structure	137:223	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	1	36	theme	certain	137:143	arg1	biodegradation					170:183	biodegradation	170:183	biodegradation	170:183	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	1	36	theme	certain	137:143	arg1	structure					215:223	porous structure	208:223	porous structure	208:223	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	7	37	theme	potential	1051:1059	arg1	candidate					1061:1069	a potential candidate	1049:1069	a potential candidate to be used for tissue engineering	1049:1103	Thus, we suggest that SF/CS/Nano ZrO2 composite scaffold is a potential candidate to be used for tissue engineering.
25709014	7	37	theme	potential	1051:1059	arg1	scaffold					1037:1044	SF/CS/Nano ZrO2 composite scaffold	1011:1044	SF/CS/Nano ZrO2 composite scaffold	1011:1044	Thus, we suggest that SF/CS/Nano ZrO2 composite scaffold is a potential candidate to be used for tissue engineering.
25709014	1	38	theme	desired	145:151	arg1	biocompatibility					186:201	biocompatibility	186:201	biocompatibility	186:201	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	1	38	theme	desired	145:151	arg1	features					153:160	certain desired features	137:160	certain desired features such as biodegradation, biocompatibility, and porous structure	137:223	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	1	38	theme	desired	145:151	arg1	biodegradation					170:183	biodegradation	170:183	biodegradation	170:183	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	1	38	theme	desired	145:151	arg1	structure					215:223	porous structure	208:223	porous structure	208:223	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
25709014	6	39	theme	Gingival	949:956	arg1	Fibroblast					958:967	the Human Gingival Fibroblast	939:967	the Human Gingival Fibroblast (HGF, NCBI: C-131)	939:986	Cytocompatibility of the SF/CS/Nano ZrO2 scaffold, assessed by MTT assay, revealed non-toxicity to the Human Gingival Fibroblast (HGF, NCBI: C-131).
25709014	6	40	dep	HGF	970:972	arg1	C-131					981:985	C-131	981:985	C-131	981:985	Cytocompatibility of the SF/CS/Nano ZrO2 scaffold, assessed by MTT assay, revealed non-toxicity to the Human Gingival Fibroblast (HGF, NCBI: C-131).
25709014	5	41	theme	compressive	745:755	arg1	strength					757:764	compressive strength	745:764	compressive strength	745:764	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	6	42	theme	Human	943:947	arg1	Fibroblast					958:967	the Human Gingival Fibroblast	939:967	the Human Gingival Fibroblast (HGF, NCBI: C-131)	939:986	Cytocompatibility of the SF/CS/Nano ZrO2 scaffold, assessed by MTT assay, revealed non-toxicity to the Human Gingival Fibroblast (HGF, NCBI: C-131).
25709014	5	43	theme	zirconia	682:689	arg1	presence					670:677	The presence	666:677	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold	666:721	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	5	44	theme	same	805:808	arg1	time					810:813	the same time	801:813	the same time	801:813	The presence of zirconia in the SF/CS/Nano ZrO2 scaffold led to an increase in compressive strength and water uptake capacity while at the same time decreasing the porosity.
25709014	7	45	theme	composite	1027:1035	arg1	candidate					1061:1069	a potential candidate	1049:1069	a potential candidate to be used for tissue engineering	1049:1103	Thus, we suggest that SF/CS/Nano ZrO2 composite scaffold is a potential candidate to be used for tissue engineering.
25709014	7	45	theme	composite	1027:1035	arg1	scaffold					1037:1044	SF/CS/Nano ZrO2 composite scaffold	1011:1044	SF/CS/Nano ZrO2 composite scaffold	1011:1044	Thus, we suggest that SF/CS/Nano ZrO2 composite scaffold is a potential candidate to be used for tissue engineering.
25709014	0	46	dep	Nano-composite	0:13	arg1	fabrication					87:97	fabrication	87:97	fabrication	87:97	Nano-composite of silk fibroin-chitosan/Nano ZrO2 for tissue engineering applications: fabrication and morphology.
25709014	0	46	dep	Nano-composite	0:13	arg1	morphology					103:112	morphology	103:112	morphology	103:112	Nano-composite of silk fibroin-chitosan/Nano ZrO2 for tissue engineering applications: fabrication and morphology.
25709014	4	47	theme	pore	601:604	arg1	dimensions					606:615	pore dimensions	601:615	pore dimensions suitable for cell infiltration and colonization	601:663	The scaffold was found to possess a porous nature with pore dimensions suitable for cell infiltration and colonization.
25709014	1	48	theme	tissue	255:260	arg1	engineering					262:272	tissue engineering	255:272	tissue engineering	255:272	A scaffold possessing certain desired features such as biodegradation, biocompatibility, and porous structure could serve as a template for tissue engineering.
28238838	6	0	with	interactions	1198:1209	arg1	aggregates					1263:1272	its aggregates	1259:1272	its aggregates	1259:1272	The latter ensures proper positioning of the functional groups to maximize the underlying interactions with both the monomeric form of α-synuclein and its aggregates.
28238838	6	0	with	interactions	1198:1209	arg1	form					1235:1238	the monomeric form	1221:1238	the monomeric form of α-synuclein	1221:1253	The latter ensures proper positioning of the functional groups to maximize the underlying interactions with both the monomeric form of α-synuclein and its aggregates.
28238838	7	1	dep	variants	1368:1375	arg1	variants					1368:1375	the other cyclodextrin variants	1345:1375	the other cyclodextrin variants [α-CD and HP-β-CD] used	1345:1399	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	7	1	dep	variants	1368:1375	arg1	HP-β-CD					1387:1393	HP-β-CD	1387:1393	HP-β-CD	1387:1393	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	7	1	dep	variants	1368:1375	arg1	[α-CD					1377:1381	[α-CD	1377:1381	[α-CD	1377:1381	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	7	2	theme	water	1455:1459	arg1	solubility					1461:1470	better water solubility	1448:1470	better water solubility	1448:1470	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	8	3	theme	viable	1637:1642	arg1	compositions					1649:1660	viable drug compositions	1637:1660	viable drug compositions in the near future	1637:1679	Moreover, the fact that the combinations remained effective under conditions of macromolecular crowding suggests that these have the potential to be developed into viable drug compositions in the near future.
28238838	2	4	with	combination	415:425	arg1	β-cyclodextrin					432:445	β-cyclodextrin	432:445	β-cyclodextrin (β-CD)	432:452	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	2	4	with	combination	415:425	arg1	β-CD					448:451	β-CD	448:451	β-CD	448:451	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	9	5	from	assays	1686:1691	arg1	viability					1701:1709	cell viability	1696:1709	cell viability	1696:1709	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	0	6	theme	mimicking	108:116	arg1	conditions					118:127	cell mimicking conditions	103:127	cell mimicking conditions: A promising therapeutic alternative	103:164	Polyphenols in combination with β-cyclodextrin can inhibit and disaggregate α-synuclein amyloids under cell mimicking conditions: A promising therapeutic alternative.
28238838	0	7	from	Polyphenols	0:10	arg1	combination					15:25	combination	15:25	combination with β-cyclodextrin	15:45	Polyphenols in combination with β-cyclodextrin can inhibit and disaggregate α-synuclein amyloids under cell mimicking conditions: A promising therapeutic alternative.
28238838	0	8	theme	cell	103:106	arg1	conditions					118:127	cell mimicking conditions	103:127	cell mimicking conditions: A promising therapeutic alternative	103:164	Polyphenols in combination with β-cyclodextrin can inhibit and disaggregate α-synuclein amyloids under cell mimicking conditions: A promising therapeutic alternative.
28238838	5	9	theme	flexibility	1095:1105	arg1	composition					1038:1048	a balanced composition	1027:1048	a balanced composition of the phenolic OH groups, benzene rings and flexibility	1027:1105	Our results suggest that the efficiency of curcumin results from a balanced composition of the phenolic OH groups, benzene rings and flexibility.
28238838	6	10	theme	underlying	1187:1196	arg1	interactions					1198:1209	the underlying interactions	1183:1209	the underlying interactions with both the monomeric form of α-synuclein and its aggregates	1183:1272	The latter ensures proper positioning of the functional groups to maximize the underlying interactions with both the monomeric form of α-synuclein and its aggregates.
28238838	0	11	dep	conditions	118:127	arg1	alternative					154:164	A promising therapeutic alternative	130:164	cell mimicking conditions: A promising therapeutic alternative	103:164	Polyphenols in combination with β-cyclodextrin can inhibit and disaggregate α-synuclein amyloids under cell mimicking conditions: A promising therapeutic alternative.
28238838	9	12	theme	N2a	1925:1927	arg1	lines					1918:1922	the mouse neuroblastoma cell lines	1889:1922	the mouse neuroblastoma cell lines (N2a cells)	1889:1934	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	9	12	theme	N2a	1925:1927	arg1	cells					1929:1933	N2a cells	1925:1933	N2a cells	1925:1933	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	8	13	theme	drug	1644:1647	arg1	compositions					1649:1660	viable drug compositions	1637:1660	viable drug compositions in the near future	1637:1679	Moreover, the fact that the combinations remained effective under conditions of macromolecular crowding suggests that these have the potential to be developed into viable drug compositions in the near future.
28238838	2	14	from	effects	364:370	arg1	aggregation					461:471	the aggregation	457:471	the aggregation of α-synuclein	457:486	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	2	14	from	effects	364:370	arg1	combination					415:425	combination	415:425	combination with β-cyclodextrin (β-CD)	415:452	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	9	15	theme	cell	1913:1916	arg1	lines					1918:1922	the mouse neuroblastoma cell lines	1889:1922	the mouse neuroblastoma cell lines (N2a cells)	1889:1934	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	9	15	theme	cell	1913:1916	arg1	cells					1929:1933	N2a cells	1925:1933	N2a cells	1925:1933	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	7	16	theme	other	1349:1353	arg1	variants					1368:1375	the other cyclodextrin variants	1345:1375	the other cyclodextrin variants [α-CD and HP-β-CD] used	1345:1399	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	7	16	theme	other	1349:1353	arg1	HP-β-CD					1387:1393	HP-β-CD	1387:1393	HP-β-CD	1387:1393	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	7	16	theme	other	1349:1353	arg1	[α-CD					1377:1381	[α-CD	1377:1381	[α-CD	1377:1381	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	2	17	theme	agents	531:536	arg1	presence					495:502	the presence	491:502	the presence of macromolecular crowding agents	491:536	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	9	18	theme	prefibrillar	1850:1861	arg1	aggregates					1875:1884	the prefibrillar α-synuclein aggregates	1846:1884	the prefibrillar α-synuclein aggregates	1846:1884	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	9	19	theme	MTT	1682:1684	arg1	assays					1686:1691	MTT assays	1682:1691	MTT assays on cell viability	1682:1709	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	4	20	dep	-epigallocatechin	856:872	arg1	-					854:854	-	854:854	-	854:854	Curcumin was found to be the most efficient, followed by baicalein with (-)-epigallocatechin gallate and resveratrol coming in next, the latter two exhibiting very similar effects.
28238838	7	21	theme	better	1448:1453	arg1	solubility					1461:1470	better water solubility	1448:1470	better water solubility	1448:1470	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	0	22	theme	α-synuclein	76:86	arg1	amyloids					88:95	α-synuclein amyloids	76:95	α-synuclein amyloids	76:95	Polyphenols in combination with β-cyclodextrin can inhibit and disaggregate α-synuclein amyloids under cell mimicking conditions: A promising therapeutic alternative.
28238838	3	23	theme	preformed	762:770	arg1	fibrils					772:778	preformed fibrils	762:778	preformed fibrils	762:778	Our results reveal that even at sub-stoichiometric concentrations of the individual components, the polyphenol-β-CD combination(s) not only inhibited the aggregation of the proteins but was also effective in disaggregating preformed fibrils.
28238838	2	24	theme	polyphenols	400:410	arg1	effects					364:370	the effects	360:370	the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein	360:486	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	3	25	theme	proteins	712:719	arg1	aggregation					693:703	the aggregation	689:703	the aggregation of the proteins	689:719	Our results reveal that even at sub-stoichiometric concentrations of the individual components, the polyphenol-β-CD combination(s) not only inhibited the aggregation of the proteins but was also effective in disaggregating preformed fibrils.
28238838	8	26	theme	macromolecular	1553:1566	arg1	crowding					1568:1575	macromolecular crowding	1553:1575	macromolecular crowding	1553:1575	Moreover, the fact that the combinations remained effective under conditions of macromolecular crowding suggests that these have the potential to be developed into viable drug compositions in the near future.
28238838	4	27	theme	-epigallocatechin	856:872	arg1	gallate					874:880	gallate	874:880	gallate	874:880	Curcumin was found to be the most efficient, followed by baicalein with (-)-epigallocatechin gallate and resveratrol coming in next, the latter two exhibiting very similar effects.
28238838	6	28	theme	groups	1164:1169	arg1	positioning					1134:1144	proper positioning	1127:1144	proper positioning of the functional groups	1127:1169	The latter ensures proper positioning of the functional groups to maximize the underlying interactions with both the monomeric form of α-synuclein and its aggregates.
28238838	2	29	theme	occurring	390:398	arg1	polyphenols					400:410	four naturally occurring polyphenols	375:410	four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD)	375:452	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	1	30	theme	disordered	297:306	arg1	protein					308:314	an intrinsically disordered protein	280:314	an intrinsically disordered protein α-synuclein	280:326	Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein.
28238838	0	31	theme	promising	132:140	arg1	alternative					154:164	A promising therapeutic alternative	130:164	cell mimicking conditions: A promising therapeutic alternative	103:164	Polyphenols in combination with β-cyclodextrin can inhibit and disaggregate α-synuclein amyloids under cell mimicking conditions: A promising therapeutic alternative.
28238838	3	32	theme	sub-stoichiometric	571:588	arg1	concentrations					590:603	sub-stoichiometric concentrations	571:603	sub-stoichiometric concentrations of the individual components	571:632	Our results reveal that even at sub-stoichiometric concentrations of the individual components, the polyphenol-β-CD combination(s) not only inhibited the aggregation of the proteins but was also effective in disaggregating preformed fibrils.
28238838	1	33	theme	protein	308:314	arg1	α-synuclein					316:326	an intrinsically disordered protein α-synuclein	280:326	an intrinsically disordered protein α-synuclein	280:326	Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein.
28238838	1	34	theme	α-synuclein	316:326	arg1	aggregates					248:257	insoluble and neurotoxic aggregates	223:257	aggregates	248:257	Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein.
28238838	1	34	theme	α-synuclein	316:326	arg1	fibrils					268:274	amyloid fibrils	260:274	amyloid fibrils	260:274	Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein.
28238838	4	35	theme	similar	945:951	arg1	effects					953:959	very similar effects	940:959	very similar effects	940:959	Curcumin was found to be the most efficient, followed by baicalein with (-)-epigallocatechin gallate and resveratrol coming in next, the latter two exhibiting very similar effects.
28238838	5	36	theme	benzene	1077:1083	arg1	rings					1085:1089	benzene rings	1077:1089	benzene rings	1077:1089	Our results suggest that the efficiency of curcumin results from a balanced composition of the phenolic OH groups, benzene rings and flexibility.
28238838	9	37	theme	mouse	1893:1897	arg1	lines					1918:1922	the mouse neuroblastoma cell lines	1889:1922	the mouse neuroblastoma cell lines (N2a cells)	1889:1934	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	9	37	theme	mouse	1893:1897	arg1	cells					1929:1933	N2a cells	1925:1933	N2a cells	1925:1933	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	1	38	theme	insoluble	223:231	arg1	aggregates					248:257	insoluble and neurotoxic aggregates	223:257	aggregates	248:257	Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein.
28238838	1	38	theme	insoluble	223:231	arg1	fibrils					268:274	amyloid fibrils	260:274	amyloid fibrils	260:274	Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein.
28238838	0	39	theme	therapeutic	142:152	arg1	alternative					154:164	A promising therapeutic alternative	130:164	cell mimicking conditions: A promising therapeutic alternative	103:164	Polyphenols in combination with β-cyclodextrin can inhibit and disaggregate α-synuclein amyloids under cell mimicking conditions: A promising therapeutic alternative.
28238838	5	40	theme	rings	1085:1089	arg1	composition					1038:1048	a balanced composition	1027:1048	a balanced composition of the phenolic OH groups, benzene rings and flexibility	1027:1105	Our results suggest that the efficiency of curcumin results from a balanced composition of the phenolic OH groups, benzene rings and flexibility.
28238838	5	41	theme	balanced	1029:1036	arg1	composition					1038:1048	a balanced composition	1027:1048	a balanced composition of the phenolic OH groups, benzene rings and flexibility	1027:1105	Our results suggest that the efficiency of curcumin results from a balanced composition of the phenolic OH groups, benzene rings and flexibility.
28238838	2	42	from	polyphenols	400:410	arg1	combination					415:425	combination	415:425	combination with β-cyclodextrin (β-CD)	415:452	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	2	43	theme	α-synuclein	476:486	arg1	aggregation					461:471	the aggregation	457:471	the aggregation of α-synuclein	457:486	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	8	44	from	compositions	1649:1660	arg1	future					1674:1679	future	1674:1679	future	1674:1679	Moreover, the fact that the combinations remained effective under conditions of macromolecular crowding suggests that these have the potential to be developed into viable drug compositions in the near future.
28238838	9	45	dep	confirmed	1725:1733	arg1	wherein					1751:1757	wherein	1751:1757	confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells)	1725:1934	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	3	46	theme	individual	612:621	arg1	components					623:632	the individual components	608:632	the individual components	608:632	Our results reveal that even at sub-stoichiometric concentrations of the individual components, the polyphenol-β-CD combination(s) not only inhibited the aggregation of the proteins but was also effective in disaggregating preformed fibrils.
28238838	0	47	with	combination	15:25	arg1	β-cyclodextrin					32:45	β-cyclodextrin	32:45	β-cyclodextrin	32:45	Polyphenols in combination with β-cyclodextrin can inhibit and disaggregate α-synuclein amyloids under cell mimicking conditions: A promising therapeutic alternative.
28238838	7	48	theme	β-CD	1293:1296	arg1	uniqueness					1279:1288	The uniqueness	1275:1288	The uniqueness of β-CD	1275:1296	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	9	49	theme	α-synuclein	1863:1873	arg1	aggregates					1875:1884	the prefibrillar α-synuclein aggregates	1846:1884	the prefibrillar α-synuclein aggregates	1846:1884	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	9	50	theme	cell	1696:1699	arg1	viability					1701:1709	cell viability	1696:1709	cell viability	1696:1709	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	8	51	theme	crowding	1568:1575	arg1	conditions					1539:1548	conditions	1539:1548	conditions of macromolecular crowding	1539:1575	Moreover, the fact that the combinations remained effective under conditions of macromolecular crowding suggests that these have the potential to be developed into viable drug compositions in the near future.
28238838	3	52	theme	components	623:632	arg1	concentrations					590:603	sub-stoichiometric concentrations	571:603	sub-stoichiometric concentrations of the individual components	571:632	Our results reveal that even at sub-stoichiometric concentrations of the individual components, the polyphenol-β-CD combination(s) not only inhibited the aggregation of the proteins but was also effective in disaggregating preformed fibrils.
28238838	2	53	from	combination	415:425	arg1	effects					364:370	the effects	360:370	the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein	360:486	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	7	54	contain	possessing	1437:1446	arg2	solubility					1461:1470	better water solubility	1448:1470	better water solubility	1448:1470	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	7	54	contain	possessing	1437:1446	arg1	these					1431:1435	these	1431:1435	these	1431:1435	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	9	55	theme	aggregates	1875:1884	arg1	toxicity					1834:1841	the toxicity	1830:1841	these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates	1759:1884	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	7	56	theme	cyclodextrin	1355:1366	arg1	variants					1368:1375	the other cyclodextrin variants	1345:1375	the other cyclodextrin variants [α-CD and HP-β-CD] used	1345:1399	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	7	56	theme	cyclodextrin	1355:1366	arg1	HP-β-CD					1387:1393	HP-β-CD	1387:1393	HP-β-CD	1387:1393	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	7	56	theme	cyclodextrin	1355:1366	arg1	[α-CD					1377:1381	[α-CD	1377:1381	[α-CD	1377:1381	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	5	57	theme	phenolic	1057:1064	arg1	groups					1069:1074	the phenolic OH groups	1053:1074	the phenolic OH groups	1053:1074	Our results suggest that the efficiency of curcumin results from a balanced composition of the phenolic OH groups, benzene rings and flexibility.
28238838	6	58	theme	monomeric	1225:1233	arg1	form					1235:1238	the monomeric form	1221:1238	the monomeric form of α-synuclein	1221:1253	The latter ensures proper positioning of the functional groups to maximize the underlying interactions with both the monomeric form of α-synuclein and its aggregates.
28238838	6	59	theme	α-synuclein	1243:1253	arg1	aggregates					1263:1272	its aggregates	1259:1272	its aggregates	1259:1272	The latter ensures proper positioning of the functional groups to maximize the underlying interactions with both the monomeric form of α-synuclein and its aggregates.
28238838	6	59	theme	α-synuclein	1243:1253	arg1	form					1235:1238	the monomeric form	1221:1238	the monomeric form of α-synuclein	1221:1253	The latter ensures proper positioning of the functional groups to maximize the underlying interactions with both the monomeric form of α-synuclein and its aggregates.
28238838	6	60	theme	proper	1127:1132	arg1	positioning					1134:1144	proper positioning	1127:1144	proper positioning of the functional groups	1127:1169	The latter ensures proper positioning of the functional groups to maximize the underlying interactions with both the monomeric form of α-synuclein and its aggregates.
28238838	6	61	theme	functional	1153:1162	arg1	groups					1164:1169	the functional groups	1149:1169	the functional groups	1149:1169	The latter ensures proper positioning of the functional groups to maximize the underlying interactions with both the monomeric form of α-synuclein and its aggregates.
28238838	1	62	theme	neurotoxic	237:246	arg1	aggregates					248:257	insoluble and neurotoxic aggregates	223:257	aggregates	248:257	Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein.
28238838	1	62	theme	neurotoxic	237:246	arg1	fibrils					268:274	amyloid fibrils	260:274	amyloid fibrils	260:274	Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein.
28238838	5	63	theme	OH	1066:1067	arg1	groups					1069:1074	the phenolic OH groups	1053:1074	the phenolic OH groups	1053:1074	Our results suggest that the efficiency of curcumin results from a balanced composition of the phenolic OH groups, benzene rings and flexibility.
28238838	5	64	theme	curcumin	1005:1012	arg1	efficiency					991:1000	the efficiency	987:1000	the efficiency of curcumin	987:1012	Our results suggest that the efficiency of curcumin results from a balanced composition of the phenolic OH groups, benzene rings and flexibility.
28238838	9	65	dep	combinations	1765:1776	arg1	polyphenols					1787:1797	the polyphenols	1783:1797	the polyphenols	1783:1797	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	1	66	theme	aggregates	248:257	arg1	presence					211:218	the presence	207:218	the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein	207:326	Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein.
28238838	2	67	theme	crowding	522:529	arg1	agents					531:536	macromolecular crowding agents	507:536	macromolecular crowding agents	507:536	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	5	68	theme	groups	1069:1074	arg1	composition					1038:1048	a balanced composition	1027:1048	a balanced composition of the phenolic OH groups, benzene rings and flexibility	1027:1105	Our results suggest that the efficiency of curcumin results from a balanced composition of the phenolic OH groups, benzene rings and flexibility.
28238838	3	69	theme	polyphenol-β-CD	639:653	arg1	s					667:667	the polyphenol-β-CD combination(s)	635:668	the polyphenol-β-CD combination(s)	635:668	Our results reveal that even at sub-stoichiometric concentrations of the individual components, the polyphenol-β-CD combination(s) not only inhibited the aggregation of the proteins but was also effective in disaggregating preformed fibrils.
28238838	8	70	contain	have	1597:1600	arg1	these					1591:1595	these	1591:1595	these	1591:1595	Moreover, the fact that the combinations remained effective under conditions of macromolecular crowding suggests that these have the potential to be developed into viable drug compositions in the near future.
28238838	8	70	contain	have	1597:1600	arg2	potential					1606:1614	the potential to be developed into viable drug compositions in the near future	1602:1679	the potential to be developed into viable drug compositions in the near future	1602:1679	Moreover, the fact that the combinations remained effective under conditions of macromolecular crowding suggests that these have the potential to be developed into viable drug compositions in the near future.
28238838	4	71	with	baicalein	838:846	arg1	resveratrol					886:896	resveratrol	886:896	resveratrol	886:896	Curcumin was found to be the most efficient, followed by baicalein with (-)-epigallocatechin gallate and resveratrol coming in next, the latter two exhibiting very similar effects.
28238838	4	71	with	baicalein	838:846	arg1	gallate					874:880	gallate	874:880	gallate	874:880	Curcumin was found to be the most efficient, followed by baicalein with (-)-epigallocatechin gallate and resveratrol coming in next, the latter two exhibiting very similar effects.
28238838	2	72	theme	macromolecular	507:520	arg1	agents					531:536	macromolecular crowding agents	507:536	macromolecular crowding agents	507:536	In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents.
28238838	7	73	theme	variants	1368:1375	arg1	none					1337:1340	none	1337:1340	none of the other cyclodextrin variants [α-CD and HP-β-CD] used	1337:1399	The uniqueness of β-CD was reinforced by the observation that none of the other cyclodextrin variants [α-CD and HP-β-CD] used was as effective, in spite of these possessing better water solubility.
28238838	3	74	theme	combination	655:665	arg1	s					667:667	the polyphenol-β-CD combination(s)	635:668	the polyphenol-β-CD combination(s)	635:668	Our results reveal that even at sub-stoichiometric concentrations of the individual components, the polyphenol-β-CD combination(s) not only inhibited the aggregation of the proteins but was also effective in disaggregating preformed fibrils.
28238838	3	75	dep	concentrations	590:603	arg1	only					674:677	only	674:677	only	674:677	Our results reveal that even at sub-stoichiometric concentrations of the individual components, the polyphenol-β-CD combination(s) not only inhibited the aggregation of the proteins but was also effective in disaggregating preformed fibrils.
28238838	9	76	theme	neuroblastoma	1899:1911	arg1	lines					1918:1922	the mouse neuroblastoma cell lines	1889:1922	the mouse neuroblastoma cell lines (N2a cells)	1889:1934	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	9	76	theme	neuroblastoma	1899:1911	arg1	cells					1929:1933	N2a cells	1925:1933	N2a cells	1925:1933	MTT assays on cell viability independently confirmed this hypothesis wherein these combinations (and the polyphenols alone too) appreciably impeded the toxicity of the prefibrillar α-synuclein aggregates on the mouse neuroblastoma cell lines (N2a cells).
28238838	1	77	theme	amyloid	260:266	arg1	aggregates					248:257	insoluble and neurotoxic aggregates	223:257	aggregates	248:257	Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein.
28238838	1	77	theme	amyloid	260:266	arg1	fibrils					268:274	amyloid fibrils	260:274	amyloid fibrils	260:274	Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein.
28832260	0	0	theme	mucoadhesive	105:116	arg1	patch					118:122	a buccal mucoadhesive patch	96:122	a buccal mucoadhesive patch to treat desquamative gingivitis	96:155	Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
28832260	0	0	theme	mucoadhesive	105:116	arg1	succinate					83:91	hydrocortisone sodium succinate	61:91	hydrocortisone sodium succinate	61:91	Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
28832260	8	1	contain	containing	1370:1379	arg2	lysozyme					1390:1397	104 U/ml lysozyme	1381:1397	104 U/ml lysozyme	1381:1397	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	8	1	contain	containing	1370:1379	arg1	buffer					1345:1350	phosphate buffer saline (pH = 7.4)	1335:1368	phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme	1335:1397	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	8	2	from	properties	1270:1279	arg1	buffer					1345:1350	phosphate buffer saline (pH = 7.4)	1335:1368	phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme	1335:1397	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	1	3	theme	research	174:181	arg1	aim					162:164	The aim	158:164	The aim of this research	158:181	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	0	4	theme	buccal	98:103	arg1	patch					118:122	a buccal mucoadhesive patch	96:122	a buccal mucoadhesive patch to treat desquamative gingivitis	96:155	Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
28832260	0	4	theme	buccal	98:103	arg1	succinate					83:91	hydrocortisone sodium succinate	61:91	hydrocortisone sodium succinate	61:91	Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
28832260	8	5	theme	phosphate	1335:1343	arg1	buffer					1345:1350	phosphate buffer saline (pH = 7.4)	1335:1368	phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme	1335:1397	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	10	6	theme	sodium	1604:1609	arg1	succinate					1611:1619	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate	1543:1619	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate	1543:1619	Finally, chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate can be employed in dental applications.
28832260	9	7	theme	films	1427:1431	arg1	profile					1412:1418	The release profile	1400:1418	The release profile of the films	1400:1431	The release profile of the films ascertained that keratin is a rate controller in the release of the hydrocortisone sodium succinate.
28832260	8	8	from	uptake	1306:1311	arg1	buffer					1345:1350	phosphate buffer saline (pH = 7.4)	1335:1368	phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme	1335:1397	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	4	9	theme	water	805:809	arg1	properties					774:783	the mechanical properties	759:783	the mechanical properties	759:783	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	4	9	theme	water	805:809	arg1	uptake					811:816	water uptake	805:816	water uptake	805:816	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	4	10	theme	first	682:686	arg1	step					688:691	the first step	678:691	the first step	678:691	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	5	11	theme	chitosan	940:947	arg1	chitosan					940:947	chitosan	940:947	chitosan	940:947	Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug.
28832260	5	11	theme	chitosan	940:947	arg1	composite					927:935	the optimum composite	915:935	the optimum composite of chitosan and gelatin together with the drug	915:982	Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug.
28832260	5	11	theme	chitosan	940:947	arg1	gelatin					953:959	gelatin	953:959	gelatin	953:959	Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug.
28832260	1	12	theme	desquamative	316:327	arg1	gingivitis					329:338	desquamative gingivitis	316:338	desquamative gingivitis	316:338	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	0	13	theme	desquamative	133:144	arg1	gingivitis					146:155	desquamative gingivitis	133:155	desquamative gingivitis	133:155	Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
28832260	4	14	theme	chitosan	694:701	arg1	proportions					715:725	chitosan and gelatin proportions	694:725	chitosan and gelatin proportions	694:725	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	7	15	theme	keratin	1119:1125	arg1	influence					1106:1114	the influence	1102:1114	the influence of keratin on the structure of the composite	1102:1159	FTIR analysis confirmed the influence of keratin on the structure of the composite.
28832260	3	16	theme	mucoadhesive	532:543	arg1	films					545:549	mucoadhesive films	532:549	mucoadhesive films	532:549	In this research, mucoadhesive films, for the release of hydrocortisone sodium succinate, were prepared using different ratios of chitosan, gelatin and keratin.
28832260	3	17	theme	succinate	593:601	arg1	release					560:566	the release	556:566	the release of hydrocortisone sodium succinate	556:601	In this research, mucoadhesive films, for the release of hydrocortisone sodium succinate, were prepared using different ratios of chitosan, gelatin and keratin.
28832260	1	18	theme	chitosan/gelatin/keratin	198:221	arg1	composite					223:231	chitosan/gelatin/keratin composite	198:231	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process	198:402	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	4	19	theme	gelatin	707:713	arg1	proportions					715:725	chitosan and gelatin proportions	694:725	chitosan and gelatin proportions	694:725	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	8	20	theme	higher	1180:1185	arg1	keratin					1197:1203	keratin	1197:1203	keratin	1197:1203	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	8	20	theme	higher	1180:1185	arg1	amount					1187:1192	a higher amount	1178:1192	a higher amount of keratin	1178:1203	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	8	21	theme	water	1300:1304	arg1	uptake					1306:1311	low water uptake	1296:1311	low water uptake	1296:1311	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	8	22	attach	presence	1166:1173	arg2	amount					1187:1192	a higher amount	1178:1192	a higher amount of keratin	1178:1203	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	8	22	attach	presence	1166:1173	arg1	films					1222:1226	the composite films	1208:1226	the composite films	1208:1226	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	8	22	attach	presence	1166:1173	arg2	keratin					1197:1203	keratin	1197:1203	keratin	1197:1203	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	2	23	theme	efficiency	457:466	arg1	advantage					437:445	the advantage	433:445	the advantage of higher efficiency and drug localization in the affected region	433:511	Mucoadhesive films increase the advantage of higher efficiency and drug localization in the affected region.
28832260	8	24	theme	keratin	1197:1203	arg1	keratin					1197:1203	keratin	1197:1203	keratin	1197:1203	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	8	24	theme	keratin	1197:1203	arg1	amount					1187:1192	a higher amount	1178:1192	a higher amount of keratin	1178:1203	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	10	25	theme	chitosan/gelatin/keratin	1543:1566	arg1	succinate					1611:1619	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate	1543:1619	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate	1543:1619	Finally, chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate can be employed in dental applications.
28832260	8	26	theme	104 U/ml	1381:1388	arg1	lysozyme					1390:1397	104 U/ml lysozyme	1381:1397	104 U/ml lysozyme	1381:1397	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	2	27	theme	higher	450:455	arg1	efficiency					457:466	higher efficiency	450:466	higher efficiency	450:466	Mucoadhesive films increase the advantage of higher efficiency and drug localization in the affected region.
28832260	10	28	theme	dental	1640:1645	arg1	applications					1647:1658	dental applications	1640:1658	dental applications	1640:1658	Finally, chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate can be employed in dental applications.
28832260	7	29	from	influence	1106:1114	arg1	structure					1134:1142	the structure	1130:1142	the structure of the composite	1130:1159	FTIR analysis confirmed the influence of keratin on the structure of the composite.
28832260	2	30	from	advantage	437:445	arg1	region					506:511	the affected region	493:511	the affected region	493:511	Mucoadhesive films increase the advantage of higher efficiency and drug localization in the affected region.
28832260	9	31	theme	succinate	1523:1531	arg1	release					1486:1492	the release	1482:1492	the release of the hydrocortisone sodium succinate	1482:1531	The release profile of the films ascertained that keratin is a rate controller in the release of the hydrocortisone sodium succinate.
28832260	5	32	theme	optimum	919:925	arg1	chitosan					940:947	chitosan	940:947	chitosan	940:947	Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug.
28832260	5	32	theme	optimum	919:925	arg1	composite					927:935	the optimum composite	915:935	the optimum composite of chitosan and gelatin together with the drug	915:982	Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug.
28832260	5	32	theme	optimum	919:925	arg1	gelatin					953:959	gelatin	953:959	gelatin	953:959	Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug.
28832260	0	33	theme	composite	40:48	arg1	Development					0:10	Development	0:10	Development of chitosan/gelatin/keratin composite	0:48	Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
28832260	4	34	theme	films	856:860	arg1	stability					819:827	stability	819:827	stability	819:827	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	4	34	theme	films	856:860	arg1	capacity					795:802	swelling capacity	786:802	swelling capacity	786:802	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	4	34	theme	films	856:860	arg1	uptake					811:816	water uptake	805:816	water uptake	805:816	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	4	34	theme	films	856:860	arg1	biodegradation					834:847	biodegradation	834:847	biodegradation of the films	834:860	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	4	34	theme	films	856:860	arg1	properties					774:783	the mechanical properties	759:783	the mechanical properties	759:783	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	8	35	from	biodegradation	1317:1330	arg1	buffer					1345:1350	phosphate buffer saline (pH = 7.4)	1335:1368	phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme	1335:1397	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	6	36	theme	buccal	1063:1068	arg1	cavity					1070:1075	the buccal cavity	1059:1075	the buccal cavity	1059:1075	The results of surface pH showed that none of the samples were harmful to the buccal cavity.
28832260	2	37	theme	affected	497:504	arg1	region					506:511	the affected region	493:511	the affected region	493:511	Mucoadhesive films increase the advantage of higher efficiency and drug localization in the affected region.
28832260	6	38	theme	surface	1000:1006	arg1	pH					1008:1009	surface pH	1000:1009	surface pH	1000:1009	The results of surface pH showed that none of the samples were harmful to the buccal cavity.
28832260	8	39	from	presence	1166:1173	arg1	films					1222:1226	the composite films	1208:1226	the composite films	1208:1226	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	3	40	theme	different	624:632	arg1	ratios					634:639	different ratios	624:639	different ratios of chitosan, gelatin and keratin	624:672	In this research, mucoadhesive films, for the release of hydrocortisone sodium succinate, were prepared using different ratios of chitosan, gelatin and keratin.
28832260	8	41	from	stability	1285:1293	arg1	buffer					1345:1350	phosphate buffer saline (pH = 7.4)	1335:1368	phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme	1335:1397	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	1	42	theme	hydrocortisone	244:257	arg1	patch					301:305	a buccal mucoadhesive patch	279:305	a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process	279:402	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	1	42	theme	hydrocortisone	244:257	arg1	succinate					266:274	hydrocortisone sodium succinate	244:274	hydrocortisone sodium succinate	244:274	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	0	43	theme	hydrocortisone	61:74	arg1	patch					118:122	a buccal mucoadhesive patch	96:122	a buccal mucoadhesive patch to treat desquamative gingivitis	96:155	Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
28832260	0	43	theme	hydrocortisone	61:74	arg1	succinate					83:91	hydrocortisone sodium succinate	61:91	hydrocortisone sodium succinate	61:91	Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
28832260	8	44	theme	low	1296:1298	arg1	uptake					1306:1311	low water uptake	1296:1311	low water uptake	1296:1311	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	7	45	theme	FTIR	1078:1081	arg1	analysis					1083:1090	FTIR analysis	1078:1090	FTIR analysis	1078:1090	FTIR analysis confirmed the influence of keratin on the structure of the composite.
28832260	1	46	theme	sodium	259:264	arg1	patch					301:305	a buccal mucoadhesive patch	279:305	a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process	279:402	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	1	46	theme	sodium	259:264	arg1	succinate					266:274	hydrocortisone sodium succinate	244:274	hydrocortisone sodium succinate	244:274	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	3	47	theme	gelatin	654:660	arg1	ratios					634:639	different ratios	624:639	different ratios of chitosan, gelatin and keratin	624:672	In this research, mucoadhesive films, for the release of hydrocortisone sodium succinate, were prepared using different ratios of chitosan, gelatin and keratin.
28832260	9	48	theme	rate	1463:1466	arg1	keratin					1450:1456	keratin	1450:1456	keratin	1450:1456	The release profile of the films ascertained that keratin is a rate controller in the release of the hydrocortisone sodium succinate.
28832260	9	48	theme	rate	1463:1466	arg1	controller					1468:1477	a rate controller	1461:1477	a rate controller in the release of the hydrocortisone sodium succinate	1461:1531	The release profile of the films ascertained that keratin is a rate controller in the release of the hydrocortisone sodium succinate.
28832260	2	49	theme	localization	477:488	arg1	advantage					437:445	the advantage	433:445	the advantage of higher efficiency and drug localization in the affected region	433:511	Mucoadhesive films increase the advantage of higher efficiency and drug localization in the affected region.
28832260	8	50	theme	mechanical	1245:1254	arg1	properties					1270:1279	high mechanical, mucoadhesive properties	1240:1279	properties	1270:1279	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	8	51	theme	composite	1212:1220	arg1	films					1222:1226	the composite films	1208:1226	the composite films	1208:1226	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	6	52	theme	samples	1035:1041	arg1	none					1023:1026	none	1023:1026	none of the samples	1023:1041	The results of surface pH showed that none of the samples were harmful to the buccal cavity.
28832260	5	53	theme	different	890:898	arg1	percentages					900:910	different percentages	890:910	different percentages	890:910	Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug.
28832260	2	54	theme	drug	472:475	arg1	localization					477:488	drug localization	472:488	drug localization	472:488	Mucoadhesive films increase the advantage of higher efficiency and drug localization in the affected region.
28832260	7	55	theme	composite	1151:1159	arg1	structure					1134:1142	the structure	1130:1142	the structure of the composite	1130:1159	FTIR analysis confirmed the influence of keratin on the structure of the composite.
28832260	6	56	theme	pH	1008:1009	arg1	results					989:995	The results	985:995	The results of surface pH	985:1009	The results of surface pH showed that none of the samples were harmful to the buccal cavity.
28832260	3	57	theme	chitosan	644:651	arg1	ratios					634:639	different ratios	624:639	different ratios of chitosan, gelatin and keratin	624:672	In this research, mucoadhesive films, for the release of hydrocortisone sodium succinate, were prepared using different ratios of chitosan, gelatin and keratin.
28832260	2	58	theme	Mucoadhesive	405:416	arg1	films					418:422	Mucoadhesive films	405:422	Mucoadhesive films	405:422	Mucoadhesive films increase the advantage of higher efficiency and drug localization in the affected region.
28832260	0	59	theme	sodium	76:81	arg1	patch					118:122	a buccal mucoadhesive patch	96:122	a buccal mucoadhesive patch to treat desquamative gingivitis	96:155	Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
28832260	0	59	theme	sodium	76:81	arg1	succinate					83:91	hydrocortisone sodium succinate	61:91	hydrocortisone sodium succinate	61:91	Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
28832260	9	60	theme	release	1404:1410	arg1	profile					1412:1418	The release profile	1400:1418	The release profile of the films	1400:1431	The release profile of the films ascertained that keratin is a rate controller in the release of the hydrocortisone sodium succinate.
28832260	10	61	theme	composite	1568:1576	arg1	succinate					1611:1619	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate	1543:1619	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate	1543:1619	Finally, chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate can be employed in dental applications.
28832260	9	62	from	controller	1468:1477	arg1	release					1486:1492	the release	1482:1492	the release of the hydrocortisone sodium succinate	1482:1531	The release profile of the films ascertained that keratin is a rate controller in the release of the hydrocortisone sodium succinate.
28832260	4	63	theme	mechanical	763:772	arg1	properties					774:783	the mechanical properties	759:783	the mechanical properties	759:783	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	4	63	theme	mechanical	763:772	arg1	capacity					795:802	swelling capacity	786:802	swelling capacity	786:802	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	4	63	theme	mechanical	763:772	arg1	uptake					811:816	water uptake	805:816	water uptake	805:816	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	4	63	theme	mechanical	763:772	arg1	biodegradation					834:847	biodegradation	834:847	biodegradation of the films	834:860	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	4	63	theme	mechanical	763:772	arg1	stability					819:827	stability	819:827	stability	819:827	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	1	64	theme	environmental	373:385	arg1	process					396:402	an environmental friendly process	370:402	an environmental friendly process	370:402	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	8	65	theme	mucoadhesive	1257:1268	arg1	properties					1270:1279	high mechanical, mucoadhesive properties	1240:1279	properties	1270:1279	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	4	66	theme	swelling	786:793	arg1	properties					774:783	the mechanical properties	759:783	the mechanical properties	759:783	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	4	66	theme	swelling	786:793	arg1	capacity					795:802	swelling capacity	786:802	swelling capacity	786:802	In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films.
28832260	5	67	theme	gelatin	953:959	arg1	chitosan					940:947	chitosan	940:947	chitosan	940:947	Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug.
28832260	5	67	theme	gelatin	953:959	arg1	composite					927:935	the optimum composite	915:935	the optimum composite of chitosan and gelatin together with the drug	915:982	Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug.
28832260	5	67	theme	gelatin	953:959	arg1	gelatin					953:959	gelatin	953:959	gelatin	953:959	Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug.
28832260	9	68	theme	sodium	1516:1521	arg1	succinate					1523:1531	the hydrocortisone sodium succinate	1497:1531	the hydrocortisone sodium succinate	1497:1531	The release profile of the films ascertained that keratin is a rate controller in the release of the hydrocortisone sodium succinate.
28832260	9	69	theme	hydrocortisone	1501:1514	arg1	succinate					1523:1531	the hydrocortisone sodium succinate	1497:1531	the hydrocortisone sodium succinate	1497:1531	The release profile of the films ascertained that keratin is a rate controller in the release of the hydrocortisone sodium succinate.
28832260	10	70	theme	hydrocortisone	1589:1602	arg1	succinate					1611:1619	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate	1543:1619	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate	1543:1619	Finally, chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate can be employed in dental applications.
28832260	1	71	contain	containing	233:242	arg2	succinate					266:274	hydrocortisone sodium succinate	244:274	hydrocortisone sodium succinate	244:274	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	1	71	contain	containing	233:242	arg1	composite					223:231	chitosan/gelatin/keratin composite	198:231	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process	198:402	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	1	71	contain	containing	233:242	arg2	patch					301:305	a buccal mucoadhesive patch	279:305	a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process	279:402	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	3	72	theme	keratin	666:672	arg1	ratios					634:639	different ratios	624:639	different ratios of chitosan, gelatin and keratin	624:672	In this research, mucoadhesive films, for the release of hydrocortisone sodium succinate, were prepared using different ratios of chitosan, gelatin and keratin.
28832260	8	73	theme	amount	1187:1192	arg1	presence					1166:1173	The presence	1162:1173	The presence of a higher amount of keratin in the composite films	1162:1226	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	3	74	theme	hydrocortisone	571:584	arg1	succinate					593:601	hydrocortisone sodium succinate	571:601	hydrocortisone sodium succinate	571:601	In this research, mucoadhesive films, for the release of hydrocortisone sodium succinate, were prepared using different ratios of chitosan, gelatin and keratin.
28832260	8	75	theme	high	1240:1243	arg1	properties					1270:1279	high mechanical, mucoadhesive properties	1240:1279	properties	1270:1279	The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 104 U/ml lysozyme.
28832260	1	76	theme	buccal	281:286	arg1	patch					301:305	a buccal mucoadhesive patch	279:305	a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process	279:402	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	1	76	theme	buccal	281:286	arg1	succinate					266:274	hydrocortisone sodium succinate	244:274	hydrocortisone sodium succinate	244:274	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	10	77	theme	containing	1578:1587	arg1	succinate					1611:1619	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate	1543:1619	chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate	1543:1619	Finally, chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate can be employed in dental applications.
28832260	1	78	theme	friendly	387:394	arg1	process					396:402	an environmental friendly process	370:402	an environmental friendly process	370:402	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	0	79	theme	chitosan/gelatin/keratin	15:38	arg1	composite					40:48	chitosan/gelatin/keratin composite	15:48	chitosan/gelatin/keratin composite	15:48	Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
28832260	3	80	theme	sodium	586:591	arg1	succinate					593:601	hydrocortisone sodium succinate	571:601	hydrocortisone sodium succinate	571:601	In this research, mucoadhesive films, for the release of hydrocortisone sodium succinate, were prepared using different ratios of chitosan, gelatin and keratin.
28832260	1	81	theme	mucoadhesive	288:299	arg1	patch					301:305	a buccal mucoadhesive patch	279:305	a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process	279:402	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
28832260	1	81	theme	mucoadhesive	288:299	arg1	succinate					266:274	hydrocortisone sodium succinate	244:274	hydrocortisone sodium succinate	244:274	The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process.
27889343	5	0	theme	thermodynamic	554:566	arg1	parameters					568:577	thermodynamic parameters	554:577	thermodynamic parameters	554:577	The adsorption isotherm, kinetics and thermodynamic parameters were studied.
27889343	8	1	theme	evaluated	768:776	arg1	parameters					792:801	The evaluated thermodynamic parameters	764:801	The evaluated thermodynamic parameters	764:801	The evaluated thermodynamic parameters show a spontaneous and exothermic reaction.
27889343	1	2	theme	low	197:199	arg1	cost					201:204	a novel, low cost	188:204	cost	201:204	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	9	3	theme	TLAC/Chitosan	877:889	arg1	composite					891:899	TLAC/Chitosan composite	877:899	TLAC/Chitosan composite	877:899	Overall, this study indicates TLAC/Chitosan composite as an effective adsorbent for the removal of crystal violet dye from aqueous solutions.
27889343	9	3	theme	TLAC/Chitosan	877:889	arg1	effective					907:915	effective	907:915	effective	907:915	Overall, this study indicates TLAC/Chitosan composite as an effective adsorbent for the removal of crystal violet dye from aqueous solutions.
27889343	3	4	theme	aqueous	424:430	arg1	solutions					432:440	aqueous solutions	424:440	aqueous solutions	424:440	Its effectivity was tested for the removal of crystal violet dye from aqueous solutions.
27889343	4	5	theme	initial	475:481	arg1	concentration					487:499	initial dye concentration	475:499	initial dye concentration	475:499	The effect of pH, dose rate and initial dye concentration was evaluated.
27889343	0	6	theme	TLAC/Chitosan	79:91	arg1	composite					93:101	TLAC/Chitosan composite	79:101	TLAC/Chitosan composite	79:101	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite: A novel low cost adsorbent.
27889343	3	7	from	solutions	432:440	arg1	removal					389:395	the removal	385:395	the removal of crystal violet dye from aqueous solutions	385:440	Its effectivity was tested for the removal of crystal violet dye from aqueous solutions.
27889343	7	8	theme	order	748:752	arg1	kinetics					754:761	pseudo-second order kinetics	734:761	pseudo-second order kinetics	734:761	The adsorption followed pseudo-second order kinetics.
27889343	8	9	theme	spontaneous	810:820	arg1	reaction					837:844	a spontaneous and exothermic reaction	808:844	a spontaneous and exothermic reaction	808:844	The evaluated thermodynamic parameters show a spontaneous and exothermic reaction.
27889343	4	10	theme	concentration	487:499	arg1	effect					447:452	The effect	443:452	The effect of pH, dose rate and initial dye concentration	443:499	The effect of pH, dose rate and initial dye concentration was evaluated.
27889343	5	11	theme	adsorption	520:529	arg1	isotherm					531:538	The adsorption isotherm	516:538	The adsorption isotherm	516:538	The adsorption isotherm, kinetics and thermodynamic parameters were studied.
27889343	9	12	theme	dye	961:963	arg1	removal					935:941	the removal	931:941	the removal of crystal violet dye from aqueous solutions	931:986	Overall, this study indicates TLAC/Chitosan composite as an effective adsorbent for the removal of crystal violet dye from aqueous solutions.
27889343	4	13	theme	dye	483:485	arg1	concentration					487:499	initial dye concentration	475:499	initial dye concentration	475:499	The effect of pH, dose rate and initial dye concentration was evaluated.
27889343	8	14	theme	exothermic	826:835	arg1	reaction					837:844	a spontaneous and exothermic reaction	808:844	a spontaneous and exothermic reaction	808:844	The evaluated thermodynamic parameters show a spontaneous and exothermic reaction.
27889343	1	15	theme	Typha	147:151	arg1	TLAC					181:184	TLAC	181:184	TLAC	181:184	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	1	15	theme	Typha	147:151	arg1	carbon					173:178	Typha latifolia activated carbon	147:178	Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent)	147:215	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	0	16	theme	efficient	3:11	arg1	removal					13:19	An efficient removal	0:19	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite	0:101	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite: A novel low cost adsorbent.
27889343	4	17	theme	rate	466:469	arg1	effect					447:452	The effect	443:452	The effect of pH, dose rate and initial dye concentration	443:499	The effect of pH, dose rate and initial dye concentration was evaluated.
27889343	9	18	from	solutions	978:986	arg1	removal					935:941	the removal	931:941	the removal of crystal violet dye from aqueous solutions	931:986	Overall, this study indicates TLAC/Chitosan composite as an effective adsorbent for the removal of crystal violet dye from aqueous solutions.
27889343	6	19	theme	isotherm	617:624	arg1	models					626:631	Langmuir and Freundlich isotherm models	593:631	models	626:631	Langmuir and Freundlich isotherm models were found fit effectively for the dye adsorption data in the present study.
27889343	4	20	theme	dose	461:464	arg1	rate					466:469	dose rate	461:469	dose rate	461:469	The effect of pH, dose rate and initial dye concentration was evaluated.
27889343	6	21	theme	present	695:701	arg1	study					703:707	the present study	691:707	the present study	691:707	Langmuir and Freundlich isotherm models were found fit effectively for the dye adsorption data in the present study.
27889343	6	22	theme	Freundlich	606:615	arg1	models					626:631	Langmuir and Freundlich isotherm models	593:631	models	626:631	Langmuir and Freundlich isotherm models were found fit effectively for the dye adsorption data in the present study.
27889343	1	23	theme	activated	163:171	arg1	TLAC					181:184	TLAC	181:184	TLAC	181:184	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	1	23	theme	activated	163:171	arg1	carbon					173:178	Typha latifolia activated carbon	147:178	Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent)	147:215	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	1	24	theme	chitosan	221:228	arg1	chitosan					221:228	chitosan	221:228	chitosan (TLAC/Chitosan composite)	221:254	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	1	24	theme	chitosan	221:228	arg1	carbon					173:178	Typha latifolia activated carbon	147:178	Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent)	147:215	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	1	24	theme	chitosan	221:228	arg1	composite					134:142	A composite	132:142	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite)	132:254	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	0	25	theme	novel	106:110	arg1	cost					116:119	A novel low cost adsorbent	104:129	A novel low cost adsorbent	104:129	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite: A novel low cost adsorbent.
27889343	1	26	theme	carbon	173:178	arg1	chitosan					221:228	chitosan	221:228	chitosan (TLAC/Chitosan composite)	221:254	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	1	26	theme	carbon	173:178	arg1	carbon					173:178	Typha latifolia activated carbon	147:178	Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent)	147:215	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	1	26	theme	carbon	173:178	arg1	composite					134:142	A composite	132:142	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite)	132:254	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	6	27	theme	Langmuir	593:600	arg1	models					626:631	Langmuir and Freundlich isotherm models	593:631	models	626:631	Langmuir and Freundlich isotherm models were found fit effectively for the dye adsorption data in the present study.
27889343	2	28	theme	size	340:343	arg1	studies					345:351	Pore size studies	335:351	Pore size studies	335:351	The composite was characterised using IR spectra, XRD, FESEM and Pore size studies.
27889343	0	29	theme	violet	32:37	arg1	dye					39:41	crystal violet dye	24:41	crystal violet dye	24:41	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite: A novel low cost adsorbent.
27889343	1	30	theme	TLAC/Chitosan	231:243	arg1	chitosan					221:228	chitosan	221:228	chitosan (TLAC/Chitosan composite)	221:254	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	1	30	theme	TLAC/Chitosan	231:243	arg1	composite					245:253	TLAC/Chitosan composite	231:253	TLAC/Chitosan composite	231:253	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	2	31	theme	Pore	335:338	arg1	studies					345:351	Pore size studies	335:351	Pore size studies	335:351	The composite was characterised using IR spectra, XRD, FESEM and Pore size studies.
27889343	0	32	theme	crystal	24:30	arg1	dye					39:41	crystal violet dye	24:41	crystal violet dye	24:41	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite: A novel low cost adsorbent.
27889343	0	33	theme	low	112:114	arg1	cost					116:119	A novel low cost adsorbent	104:129	A novel low cost adsorbent	104:129	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite: A novel low cost adsorbent.
27889343	0	34	from	water	54:58	arg1	removal					13:19	An efficient removal	0:19	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite	0:101	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite: A novel low cost adsorbent.
27889343	9	35	theme	violet	954:959	arg1	dye					961:963	crystal violet dye	946:963	crystal violet dye	946:963	Overall, this study indicates TLAC/Chitosan composite as an effective adsorbent for the removal of crystal violet dye from aqueous solutions.
27889343	7	36	theme	pseudo-second	734:746	arg1	kinetics					754:761	pseudo-second order kinetics	734:761	pseudo-second order kinetics	734:761	The adsorption followed pseudo-second order kinetics.
27889343	3	37	theme	violet	408:413	arg1	dye					415:417	crystal violet dye	400:417	crystal violet dye	400:417	Its effectivity was tested for the removal of crystal violet dye from aqueous solutions.
27889343	0	38	theme	dye	39:41	arg1	removal					13:19	An efficient removal	0:19	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite	0:101	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite: A novel low cost adsorbent.
27889343	4	39	theme	pH	457:458	arg1	effect					447:452	The effect	443:452	The effect of pH, dose rate and initial dye concentration	443:499	The effect of pH, dose rate and initial dye concentration was evaluated.
27889343	0	40	theme	adsorbent	121:129	arg1	cost					116:119	A novel low cost adsorbent	104:129	A novel low cost adsorbent	104:129	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite: A novel low cost adsorbent.
27889343	9	41	theme	crystal	946:952	arg1	dye					961:963	crystal violet dye	946:963	crystal violet dye	946:963	Overall, this study indicates TLAC/Chitosan composite as an effective adsorbent for the removal of crystal violet dye from aqueous solutions.
27889343	1	42	dep	Typha	147:151	arg1	latifolia					153:161	latifolia	153:161	latifolia	153:161	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	6	43	theme	adsorption	672:681	arg1	data					683:686	the dye adsorption data	664:686	the dye adsorption data in the present study	664:707	Langmuir and Freundlich isotherm models were found fit effectively for the dye adsorption data in the present study.
27889343	3	44	theme	dye	415:417	arg1	removal					389:395	the removal	385:395	the removal of crystal violet dye from aqueous solutions	385:440	Its effectivity was tested for the removal of crystal violet dye from aqueous solutions.
27889343	6	45	theme	dye	668:670	arg1	data					683:686	the dye adsorption data	664:686	the dye adsorption data in the present study	664:707	Langmuir and Freundlich isotherm models were found fit effectively for the dye adsorption data in the present study.
27889343	9	46	theme	aqueous	970:976	arg1	solutions					978:986	aqueous solutions	970:986	aqueous solutions	970:986	Overall, this study indicates TLAC/Chitosan composite as an effective adsorbent for the removal of crystal violet dye from aqueous solutions.
27889343	3	47	theme	crystal	400:406	arg1	dye					415:417	crystal violet dye	400:417	crystal violet dye	400:417	Its effectivity was tested for the removal of crystal violet dye from aqueous solutions.
27889343	1	48	dep	carbon	173:178	arg1	absorbent					206:214	absorbent	206:214	absorbent	206:214	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	0	49	dep	cost	116:119	arg1	removal					13:19	An efficient removal	0:19	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite	0:101	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite: A novel low cost adsorbent.
27889343	1	50	theme	novel	190:194	arg1	cost					201:204	a novel, low cost	188:204	cost	201:204	A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared.
27889343	0	51	theme	waste	48:52	arg1	water					54:58	waste water	48:58	waste water	48:58	An efficient removal of crystal violet dye from waste water by adsorption onto TLAC/Chitosan composite: A novel low cost adsorbent.
27889343	2	52	theme	IR	308:309	arg1	spectra					311:317	IR spectra	308:317	IR spectra	308:317	The composite was characterised using IR spectra, XRD, FESEM and Pore size studies.
27889343	8	53	theme	thermodynamic	778:790	arg1	parameters					792:801	The evaluated thermodynamic parameters	764:801	The evaluated thermodynamic parameters	764:801	The evaluated thermodynamic parameters show a spontaneous and exothermic reaction.
27889343	6	54	from	data	683:686	arg1	study					703:707	the present study	691:707	the present study	691:707	Langmuir and Freundlich isotherm models were found fit effectively for the dye adsorption data in the present study.
28385242	6	0	theme	chitosan/poly	1025:1037	arg1	bilayer					1053:1059	the gallium-modified chitosan/poly(acrylic acid) bilayer	1004:1059	the gallium-modified chitosan/poly(acrylic acid) bilayer	1004:1059	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	6	0	theme	chitosan/poly	1025:1037	arg1	coating					1102:1108	a promising titanium coating	1081:1108	a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential	1081:1200	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	5	1	theme	developed	803:811	arg1	coating					827:833	the developed antibacterial coating	799:833	the developed antibacterial coating	799:833	Our results evidenced that the developed antibacterial coating did not considerably alter the mechanical flexural properties of titanium substrates and, in addition, influenced positively MG63 adhesion and proliferation.
28385242	3	2	theme	best	559:562	arg1	balance					564:570	the best balance	555:570	the best balance between antibacterial activity and cytocompatibility	555:623	Gallium loading into the bilayer was carefully tuned by the electrochemical deposition time to ensure the best balance between antibacterial activity and cytocompatibility.
28385242	5	3	dep	alter	856:860	arg1	influenced					938:947	influenced	938:947	influenced positively MG63 adhesion and proliferation	938:990	Our results evidenced that the developed antibacterial coating did not considerably alter the mechanical flexural properties of titanium substrates and, in addition, influenced positively MG63 adhesion and proliferation.
28385242	6	4	theme	osseointegrative	1175:1190	arg1	potential					1192:1200	osseointegrative potential	1175:1200	osseointegrative potential	1175:1200	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	4	5	theme	log	743:745	arg1	cfu/sheet					747:755	2 and 3 log cfu/sheet	735:755	2 and 3 log cfu/sheet	735:755	The 30min deposition time was able to reduce in vitro the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet, respectively.
28385242	4	6	theme	viable	684:689	arg1	counts					696:701	the viable cell counts	680:701	the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet	680:755	The 30min deposition time was able to reduce in vitro the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet, respectively.
28385242	2	7	theme	antibacterial	291:303	arg1	properties					305:314	The bilayer in vitro antibacterial properties	270:314	The bilayer in vitro antibacterial properties	270:314	The bilayer in vitro antibacterial properties and biocompatibility were evaluated against Escherichia coli and Pseudomonas aeruginosa and on MG63 osteoblast-like cells, respectively.
28385242	6	8	theme	bacterial	1119:1127	arg1	adhesion					1129:1136	bacterial adhesion	1119:1136	bacterial adhesion	1119:1136	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	4	9	theme	coli	709:712	arg1	counts					696:701	the viable cell counts	680:701	the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet	680:755	The 30min deposition time was able to reduce in vitro the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet, respectively.
28385242	3	10	theme	Gallium	453:459	arg1	loading					461:467	Gallium loading	453:467	Gallium loading into the bilayer	453:484	Gallium loading into the bilayer was carefully tuned by the electrochemical deposition time to ensure the best balance between antibacterial activity and cytocompatibility.
28385242	1	11	theme	electrochemical	177:191	arg1	techniques					193:202	electrochemical techniques	177:202	electrochemical techniques on titanium	177:214	A gallium-modified chitosan/poly(acrylic acid) bilayer was obtained by electrochemical techniques on titanium to reduce orthopaedic and/or dental implants failure.
28385242	0	12	theme	chitosan/poly	17:29	arg1	coatings					53:60	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings	0:60	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings for improved titanium	0:82	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings for improved titanium implant performances.
28385242	6	13	theme	gallium-modified	1008:1023	arg1	chitosan/poly					1025:1037	gallium-modified chitosan/poly	1008:1037	the gallium-modified chitosan/poly(acrylic acid) bilayer	1004:1059	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	6	13	theme	gallium-modified	1008:1023	arg1	acid					1047:1050	acrylic acid	1039:1050	acrylic acid	1039:1050	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	2	14	dep	in	282:283	arg1	vitro					285:289	vitro	285:289	vitro	285:289	The bilayer in vitro antibacterial properties and biocompatibility were evaluated against Escherichia coli and Pseudomonas aeruginosa and on MG63 osteoblast-like cells, respectively.
28385242	0	15	theme	Gallium-modified	0:15	arg1	coatings					53:60	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings	0:60	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings for improved titanium	0:82	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings for improved titanium implant performances.
28385242	6	16	theme	acrylic	1039:1045	arg1	chitosan/poly					1025:1037	gallium-modified chitosan/poly	1008:1037	the gallium-modified chitosan/poly(acrylic acid) bilayer	1004:1059	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	6	16	theme	acrylic	1039:1045	arg1	acid					1047:1050	acrylic acid	1039:1050	acrylic acid	1039:1050	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	2	17	theme	osteoblast-like	416:430	arg1	cells					432:436	MG63 osteoblast-like cells	411:436	MG63 osteoblast-like cells	411:436	The bilayer in vitro antibacterial properties and biocompatibility were evaluated against Escherichia coli and Pseudomonas aeruginosa and on MG63 osteoblast-like cells, respectively.
28385242	0	18	theme	acrylic	31:37	arg1	acid					39:42	acrylic acid	31:42	acrylic acid	31:42	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings for improved titanium implant performances.
28385242	4	19	theme	cell	691:694	arg1	counts					696:701	the viable cell counts	680:701	the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet	680:755	The 30min deposition time was able to reduce in vitro the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet, respectively.
28385242	5	20	theme	mechanical	866:875	arg1	properties					886:895	the mechanical flexural properties	862:895	the mechanical flexural properties of titanium substrates	862:918	Our results evidenced that the developed antibacterial coating did not considerably alter the mechanical flexural properties of titanium substrates and, in addition, influenced positively MG63 adhesion and proliferation.
28385242	4	21	theme	deposition	636:645	arg1	able					656:659	able	656:659	able	656:659	The 30min deposition time was able to reduce in vitro the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet, respectively.
28385242	4	21	theme	deposition	636:645	arg1	time					647:650	The 30min deposition time	626:650	The 30min deposition time	626:650	The 30min deposition time was able to reduce in vitro the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet, respectively.
28385242	3	22	theme	antibacterial	580:592	arg1	activity					594:601	antibacterial activity	580:601	antibacterial activity	580:601	Gallium loading into the bilayer was carefully tuned by the electrochemical deposition time to ensure the best balance between antibacterial activity and cytocompatibility.
28385242	1	23	theme	gallium-modified	108:123	arg1	chitosan/poly					125:137	A gallium-modified chitosan/poly	106:137	A gallium-modified chitosan/poly(acrylic acid) bilayer	106:159	A gallium-modified chitosan/poly(acrylic acid) bilayer was obtained by electrochemical techniques on titanium to reduce orthopaedic and/or dental implants failure.
28385242	1	23	theme	gallium-modified	108:123	arg1	acid					147:150	acrylic acid	139:150	acrylic acid	139:150	A gallium-modified chitosan/poly(acrylic acid) bilayer was obtained by electrochemical techniques on titanium to reduce orthopaedic and/or dental implants failure.
28385242	3	24	theme	electrochemical	513:527	arg1	time					540:543	the electrochemical deposition time	509:543	the electrochemical deposition time to ensure the best balance between antibacterial activity and cytocompatibility	509:623	Gallium loading into the bilayer was carefully tuned by the electrochemical deposition time to ensure the best balance between antibacterial activity and cytocompatibility.
28385242	5	25	theme	antibacterial	813:825	arg1	coating					827:833	the developed antibacterial coating	799:833	the developed antibacterial coating	799:833	Our results evidenced that the developed antibacterial coating did not considerably alter the mechanical flexural properties of titanium substrates and, in addition, influenced positively MG63 adhesion and proliferation.
28385242	4	26	theme	30min	630:634	arg1	able					656:659	able	656:659	able	656:659	The 30min deposition time was able to reduce in vitro the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet, respectively.
28385242	4	26	theme	30min	630:634	arg1	time					647:650	The 30min deposition time	626:650	The 30min deposition time	626:650	The 30min deposition time was able to reduce in vitro the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet, respectively.
28385242	6	27	theme	titanium	1093:1100	arg1	coating					1102:1108	a promising titanium coating	1081:1108	a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential	1081:1200	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	6	27	theme	titanium	1093:1100	arg1	bilayer					1053:1059	the gallium-modified chitosan/poly(acrylic acid) bilayer	1004:1059	the gallium-modified chitosan/poly(acrylic acid) bilayer	1004:1059	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	5	28	theme	substrates	909:918	arg1	properties					886:895	the mechanical flexural properties	862:895	the mechanical flexural properties of titanium substrates	862:918	Our results evidenced that the developed antibacterial coating did not considerably alter the mechanical flexural properties of titanium substrates and, in addition, influenced positively MG63 adhesion and proliferation.
28385242	1	29	theme	orthopaedic	226:236	arg1	implants					252:259	orthopaedic and/or dental implants	226:259	orthopaedic and/or dental implants failure	226:267	A gallium-modified chitosan/poly(acrylic acid) bilayer was obtained by electrochemical techniques on titanium to reduce orthopaedic and/or dental implants failure.
28385242	6	30	theme	promising	1083:1091	arg1	coating					1102:1108	a promising titanium coating	1081:1108	a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential	1081:1200	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	6	30	theme	promising	1083:1091	arg1	bilayer					1053:1059	the gallium-modified chitosan/poly(acrylic acid) bilayer	1004:1059	the gallium-modified chitosan/poly(acrylic acid) bilayer	1004:1059	Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
28385242	1	31	theme	chitosan/poly	125:137	arg1	bilayer					153:159	A gallium-modified chitosan/poly(acrylic acid) bilayer	106:159	A gallium-modified chitosan/poly(acrylic acid) bilayer	106:159	A gallium-modified chitosan/poly(acrylic acid) bilayer was obtained by electrochemical techniques on titanium to reduce orthopaedic and/or dental implants failure.
28385242	0	32	theme	bilayer	45:51	arg1	coatings					53:60	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings	0:60	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings for improved titanium	0:82	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings for improved titanium implant performances.
28385242	3	33	theme	deposition	529:538	arg1	time					540:543	the electrochemical deposition time	509:543	the electrochemical deposition time to ensure the best balance between antibacterial activity and cytocompatibility	509:623	Gallium loading into the bilayer was carefully tuned by the electrochemical deposition time to ensure the best balance between antibacterial activity and cytocompatibility.
28385242	2	34	theme	in	282:283	arg1	properties					305:314	The bilayer in vitro antibacterial properties	270:314	The bilayer in vitro antibacterial properties	270:314	The bilayer in vitro antibacterial properties and biocompatibility were evaluated against Escherichia coli and Pseudomonas aeruginosa and on MG63 osteoblast-like cells, respectively.
28385242	1	35	theme	acrylic	139:145	arg1	chitosan/poly					125:137	A gallium-modified chitosan/poly	106:137	A gallium-modified chitosan/poly(acrylic acid) bilayer	106:159	A gallium-modified chitosan/poly(acrylic acid) bilayer was obtained by electrochemical techniques on titanium to reduce orthopaedic and/or dental implants failure.
28385242	1	35	theme	acrylic	139:145	arg1	acid					147:150	acrylic acid	139:150	acrylic acid	139:150	A gallium-modified chitosan/poly(acrylic acid) bilayer was obtained by electrochemical techniques on titanium to reduce orthopaedic and/or dental implants failure.
28385242	5	36	theme	MG63	960:963	arg1	adhesion					965:972	MG63 adhesion	960:972	MG63 adhesion	960:972	Our results evidenced that the developed antibacterial coating did not considerably alter the mechanical flexural properties of titanium substrates and, in addition, influenced positively MG63 adhesion and proliferation.
28385242	2	37	theme	bilayer	274:280	arg1	properties					305:314	The bilayer in vitro antibacterial properties	270:314	The bilayer in vitro antibacterial properties	270:314	The bilayer in vitro antibacterial properties and biocompatibility were evaluated against Escherichia coli and Pseudomonas aeruginosa and on MG63 osteoblast-like cells, respectively.
28385242	1	38	from	techniques	193:202	arg1	titanium					207:214	titanium	207:214	titanium	207:214	A gallium-modified chitosan/poly(acrylic acid) bilayer was obtained by electrochemical techniques on titanium to reduce orthopaedic and/or dental implants failure.
28385242	4	39	theme	cfu/sheet	747:755	arg1	counts					696:701	the viable cell counts	680:701	the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet	680:755	The 30min deposition time was able to reduce in vitro the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet, respectively.
28385242	1	40	theme	dental	245:250	arg1	implants					252:259	orthopaedic and/or dental implants	226:259	orthopaedic and/or dental implants failure	226:267	A gallium-modified chitosan/poly(acrylic acid) bilayer was obtained by electrochemical techniques on titanium to reduce orthopaedic and/or dental implants failure.
28385242	0	41	theme	improved	66:73	arg1	titanium					75:82	improved titanium	66:82	improved titanium	66:82	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings for improved titanium implant performances.
28385242	5	42	theme	titanium	900:907	arg1	substrates					909:918	titanium substrates	900:918	titanium substrates	900:918	Our results evidenced that the developed antibacterial coating did not considerably alter the mechanical flexural properties of titanium substrates and, in addition, influenced positively MG63 adhesion and proliferation.
28385242	0	43	dep	chitosan/poly	17:29	arg1	acid					39:42	acrylic acid	31:42	acrylic acid	31:42	Gallium-modified chitosan/poly(acrylic acid) bilayer coatings for improved titanium implant performances.
28385242	5	44	theme	flexural	877:884	arg1	properties					886:895	the mechanical flexural properties	862:895	the mechanical flexural properties of titanium substrates	862:918	Our results evidenced that the developed antibacterial coating did not considerably alter the mechanical flexural properties of titanium substrates and, in addition, influenced positively MG63 adhesion and proliferation.
28385242	2	45	theme	MG63	411:414	arg1	cells					432:436	MG63 osteoblast-like cells	411:436	MG63 osteoblast-like cells	411:436	The bilayer in vitro antibacterial properties and biocompatibility were evaluated against Escherichia coli and Pseudomonas aeruginosa and on MG63 osteoblast-like cells, respectively.
28385242	1	46	theme	implants	252:259	arg1	failure					261:267	orthopaedic and/or dental implants failure	226:267	orthopaedic and/or dental implants failure	226:267	A gallium-modified chitosan/poly(acrylic acid) bilayer was obtained by electrochemical techniques on titanium to reduce orthopaedic and/or dental implants failure.
28385242	4	47	theme	aeruginosa	721:730	arg1	counts					696:701	the viable cell counts	680:701	the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet	680:755	The 30min deposition time was able to reduce in vitro the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet, respectively.
24679493	0	0	theme	vocal	80:84	arg1	regeneration					91:102	scarless vocal fold regeneration	71:102	scarless vocal fold regeneration	71:102	Small intestine submucosa and mesenchymal stem cells composite gel for scarless vocal fold regeneration.
24679493	7	1	theme	scarred	1053:1059	arg1	VFs					1061:1063	scarred VFs	1053:1063	scarred VFs	1053:1063	In conclusion, the injectable SIS supplied a niche for the MSCs to stably settle down in scarred VFs and helped to regulate ECM synthesis.
24679493	5	2	theme	histological	728:739	arg1	analysis					741:748	The histological analysis	724:748	The histological analysis	724:748	The histological analysis showed increased hyaluronic acid accumulation and controlled collagen deposition by MSCs-SIS composite gel.
24679493	9	3	theme	MSCs	1340:1343	arg1	survival					1328:1335	prolonged survival	1318:1335	prolonged survival of MSCs	1318:1343	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	0	4	theme	scarless	71:78	arg1	regeneration					91:102	scarless vocal fold regeneration	71:102	scarless vocal fold regeneration	71:102	Small intestine submucosa and mesenchymal stem cells composite gel for scarless vocal fold regeneration.
24679493	2	5	from	injection	372:380	arg1	VF					447:448	the right side VF	432:448	the right side VF	432:448	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	7	6	theme	injectable	983:992	arg1	SIS					994:996	the injectable SIS	979:996	the injectable SIS	979:996	In conclusion, the injectable SIS supplied a niche for the MSCs to stably settle down in scarred VFs and helped to regulate ECM synthesis.
24679493	1	7	theme	scarless	149:156	arg1	regeneration					174:185	scarless vocal fold (VF) regeneration	149:185	scarless vocal fold (VF) regeneration	149:185	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	1	8	theme	small	224:228	arg1	intestine					230:238	small intestine	224:238	small intestine submucosa (SIS)	224:254	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	8	9	theme	functional	1176:1185	arg1	improvement					1187:1197	the functional improvement	1172:1197	the functional improvement of the VF	1172:1207	The ECM remodeling underwent by the surviving MSCs eventually led to the functional improvement of the VF.
24679493	5	10	theme	collagen	811:818	arg1	deposition					820:829	controlled collagen deposition	800:829	controlled collagen deposition	800:829	The histological analysis showed increased hyaluronic acid accumulation and controlled collagen deposition by MSCs-SIS composite gel.
24679493	1	11	theme	intestine	230:238	arg1	SIS					251:253	SIS	251:253	SIS	251:253	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	1	11	theme	intestine	230:238	arg1	submucosa					240:248	small intestine submucosa	224:248	small intestine submucosa (SIS)	224:254	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	0	12	theme	fold	86:89	arg1	regeneration					91:102	scarless vocal fold regeneration	71:102	scarless vocal fold regeneration	71:102	Small intestine submucosa and mesenchymal stem cells composite gel for scarless vocal fold regeneration.
24679493	5	13	theme	acid	778:781	arg1	accumulation					783:794	hyaluronic acid accumulation	767:794	hyaluronic acid accumulation	767:794	The histological analysis showed increased hyaluronic acid accumulation and controlled collagen deposition by MSCs-SIS composite gel.
24679493	6	14	theme	other	941:945	arg1	group					957:961	other treatment group	941:961	other treatment group	941:961	VKG analysis showed more favorable vibrations of MSCs-SIS injected VF, compared to other treatment group.
24679493	5	15	theme	MSCs-SIS	834:841	arg1	gel					853:855	MSCs-SIS composite gel	834:855	MSCs-SIS composite gel	834:855	The histological analysis showed increased hyaluronic acid accumulation and controlled collagen deposition by MSCs-SIS composite gel.
24679493	1	16	theme	vocal	158:162	arg1	regeneration					174:185	scarless vocal fold (VF) regeneration	149:185	scarless vocal fold (VF) regeneration	149:185	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	2	17	theme	composite	415:423	arg1	gel					425:427	MSCs-SIS composite gel	406:427	MSCs-SIS composite gel in the right side VF	406:448	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	9	18	theme	investigation	1237:1249	arg1	results					1214:1220	The results	1210:1220	The results of the present investigation	1210:1249	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	5	19	theme	composite	843:851	arg1	gel					853:855	MSCs-SIS composite gel	834:855	MSCs-SIS composite gel	834:855	The histological analysis showed increased hyaluronic acid accumulation and controlled collagen deposition by MSCs-SIS composite gel.
24679493	6	20	theme	treatment	947:955	arg1	group					957:961	other treatment group	941:961	other treatment group	941:961	VKG analysis showed more favorable vibrations of MSCs-SIS injected VF, compared to other treatment group.
24679493	1	21	theme	fold	164:167	arg1	regeneration					174:185	scarless vocal fold (VF) regeneration	149:185	scarless vocal fold (VF) regeneration	149:185	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	0	22	theme	intestine	6:14	arg1	submucosa					16:24	Small intestine submucosa	0:24	Small intestine submucosa	0:24	Small intestine submucosa and mesenchymal stem cells composite gel for scarless vocal fold regeneration.
24679493	8	23	theme	VF	1206:1207	arg1	improvement					1187:1197	the functional improvement	1172:1197	the functional improvement of the VF	1172:1207	The ECM remodeling underwent by the surviving MSCs eventually led to the functional improvement of the VF.
24679493	0	24	theme	Small	0:4	arg1	intestine					6:14	Small intestine	0:14	Small intestine submucosa	0:24	Small intestine submucosa and mesenchymal stem cells composite gel for scarless vocal fold regeneration.
24679493	1	25	theme	VF	170:171	arg1	regeneration					174:185	scarless vocal fold (VF) regeneration	149:185	scarless vocal fold (VF) regeneration	149:185	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	8	26	theme	surviving	1139:1147	arg1	MSCs					1149:1152	the surviving MSCs	1135:1152	the surviving MSCs	1135:1152	The ECM remodeling underwent by the surviving MSCs eventually led to the functional improvement of the VF.
24679493	9	27	theme	composite	1273:1281	arg1	biomaterial					1302:1312	a plausible biomaterial	1290:1312	a plausible biomaterial for prolonged survival of MSCs in VFs	1290:1350	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	9	27	theme	composite	1273:1281	arg1	gel					1283:1285	SIS-MSCs composite gel	1264:1285	SIS-MSCs composite gel	1264:1285	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	5	28	theme	controlled	800:809	arg1	deposition					820:829	controlled collagen deposition	800:829	controlled collagen deposition	800:829	The histological analysis showed increased hyaluronic acid accumulation and controlled collagen deposition by MSCs-SIS composite gel.
24679493	6	29	theme	VF	925:926	arg1	vibrations					893:902	more favorable vibrations	878:902	more favorable vibrations of MSCs-SIS injected VF	878:926	VKG analysis showed more favorable vibrations of MSCs-SIS injected VF, compared to other treatment group.
24679493	5	30	dep	showed	750:755	arg1	increased					757:765	increased	757:765	showed increased hyaluronic acid accumulation and controlled collagen deposition by MSCs-SIS composite gel	750:855	The histological analysis showed increased hyaluronic acid accumulation and controlled collagen deposition by MSCs-SIS composite gel.
24679493	3	31	theme	in	523:524	arg1	imaging					549:555	in vivo fluorescence live imaging	523:555	in vivo fluorescence live imaging system (IFLIS)	523:570	VF scars were evaluated with in vivo fluorescence live imaging system (IFLIS), endoscopy, histology, and videokymography (VKG) after eight weeks.
24679493	2	32	theme	MSCs	392:395	arg1	gel					425:427	MSCs-SIS composite gel	406:427	MSCs-SIS composite gel in the right side VF	406:448	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	2	32	theme	MSCs	392:395	arg1	SIS					398:400	SIS	398:400	SIS	398:400	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	2	32	theme	MSCs	392:395	arg1	injection					372:380	injection	372:380	injection of either MSCs	372:395	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	9	33	theme	SIS-MSCs	1264:1271	arg1	biomaterial					1302:1312	a plausible biomaterial	1290:1312	a plausible biomaterial for prolonged survival of MSCs in VFs	1290:1350	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	9	33	theme	SIS-MSCs	1264:1271	arg1	gel					1283:1285	SIS-MSCs composite gel	1264:1285	SIS-MSCs composite gel	1264:1285	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	6	34	theme	injected	916:923	arg1	VF					925:926	MSCs-SIS injected VF	907:926	MSCs-SIS injected VF	907:926	VKG analysis showed more favorable vibrations of MSCs-SIS injected VF, compared to other treatment group.
24679493	0	35	theme	stem	42:45	arg1	cells					47:51	mesenchymal stem cells	30:51	mesenchymal stem cells	30:51	Small intestine submucosa and mesenchymal stem cells composite gel for scarless vocal fold regeneration.
24679493	2	36	theme	side	442:445	arg1	VF					447:448	the right side VF	432:448	the right side VF	432:448	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	2	37	from	SIS	398:400	arg1	VF					447:448	the right side VF	432:448	the right side VF	432:448	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	6	38	theme	MSCs-SIS	907:914	arg1	VF					925:926	MSCs-SIS injected VF	907:926	MSCs-SIS injected VF	907:926	VKG analysis showed more favorable vibrations of MSCs-SIS injected VF, compared to other treatment group.
24679493	0	39	theme	mesenchymal	30:40	arg1	cells					47:51	mesenchymal stem cells	30:51	mesenchymal stem cells	30:51	Small intestine submucosa and mesenchymal stem cells composite gel for scarless vocal fold regeneration.
24679493	1	40	theme	mesenchymal	260:270	arg1	MSCs					284:287	MSCs	284:287	MSCs	284:287	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	1	40	theme	mesenchymal	260:270	arg1	cells					277:281	mesenchymal stem cells	260:281	mesenchymal stem cells (MSCs)	260:288	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	2	41	theme	right	436:440	arg1	VF					447:448	the right side VF	432:448	the right side VF	432:448	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	6	42	theme	VKG	858:860	arg1	analysis					862:869	VKG analysis	858:869	VKG analysis	858:869	VKG analysis showed more favorable vibrations of MSCs-SIS injected VF, compared to other treatment group.
24679493	2	43	from	electrocoagulator	315:331	arg1	VFs					341:343	both VFs	336:343	both VFs	336:343	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	8	44	theme	ECM	1107:1109	arg1	remodeling					1111:1120	The ECM remodeling	1103:1120	The ECM remodeling underwent by the surviving MSCs	1103:1152	The ECM remodeling underwent by the surviving MSCs eventually led to the functional improvement of the VF.
24679493	0	45	theme	composite	53:61	arg1	gel					63:65	composite gel	53:65	composite gel	53:65	Small intestine submucosa and mesenchymal stem cells composite gel for scarless vocal fold regeneration.
24679493	1	46	theme	stem	272:275	arg1	MSCs					284:287	MSCs	284:287	MSCs	284:287	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	1	46	theme	stem	272:275	arg1	cells					277:281	mesenchymal stem cells	260:281	mesenchymal stem cells (MSCs)	260:288	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	2	47	theme	side	466:469	arg1	VF					471:472	the left side VF	457:472	the left side VF	457:472	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	2	47	theme	side	466:469	arg1	untreated					483:491	untreated	483:491	untreated	483:491	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	9	48	theme	scarless	1365:1372	arg1	healing					1377:1383	scarless VF healing	1365:1383	scarless VF healing	1365:1383	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	2	49	theme	left	461:464	arg1	VF					471:472	the left side VF	457:472	the left side VF	457:472	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	2	49	theme	left	461:464	arg1	untreated					483:491	untreated	483:491	untreated	483:491	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	2	50	from	gel	425:427	arg1	VF					447:448	the right side VF	432:448	the right side VF	432:448	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	6	51	theme	favorable	883:891	arg1	vibrations					893:902	more favorable vibrations	878:902	more favorable vibrations of MSCs-SIS injected VF	878:926	VKG analysis showed more favorable vibrations of MSCs-SIS injected VF, compared to other treatment group.
24679493	5	52	theme	hyaluronic	767:776	arg1	accumulation					783:794	hyaluronic acid accumulation	767:794	hyaluronic acid accumulation	767:794	The histological analysis showed increased hyaluronic acid accumulation and controlled collagen deposition by MSCs-SIS composite gel.
24679493	9	53	from	biomaterial	1302:1312	arg1	VFs					1348:1350	VFs	1348:1350	VFs	1348:1350	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	3	54	theme	fluorescence	531:542	arg1	imaging					549:555	in vivo fluorescence live imaging	523:555	in vivo fluorescence live imaging system (IFLIS)	523:570	VF scars were evaluated with in vivo fluorescence live imaging system (IFLIS), endoscopy, histology, and videokymography (VKG) after eight weeks.
24679493	9	55	theme	VF	1374:1375	arg1	healing					1377:1383	scarless VF healing	1365:1383	scarless VF healing	1365:1383	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	1	56	theme	study	125:129	arg1	purpose					109:115	The purpose	105:115	The purpose of this study	105:129	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	1	57	theme	composite	198:206	arg1	gel					208:210	a composite gel	196:210	a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs)	196:288	The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs).
24679493	2	58	theme	MSCs-SIS	406:413	arg1	gel					425:427	MSCs-SIS composite gel	406:427	MSCs-SIS composite gel in the right side VF	406:448	A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated.
24679493	3	59	theme	live	544:547	arg1	imaging					549:555	in vivo fluorescence live imaging	523:555	in vivo fluorescence live imaging system (IFLIS)	523:570	VF scars were evaluated with in vivo fluorescence live imaging system (IFLIS), endoscopy, histology, and videokymography (VKG) after eight weeks.
24679493	9	60	theme	plausible	1292:1300	arg1	biomaterial					1302:1312	a plausible biomaterial	1290:1312	a plausible biomaterial for prolonged survival of MSCs in VFs	1290:1350	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	9	60	theme	plausible	1292:1300	arg1	gel					1283:1285	SIS-MSCs composite gel	1264:1285	SIS-MSCs composite gel	1264:1285	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	7	61	theme	ECM	1088:1090	arg1	synthesis					1092:1100	ECM synthesis	1088:1100	ECM synthesis	1088:1100	In conclusion, the injectable SIS supplied a niche for the MSCs to stably settle down in scarred VFs and helped to regulate ECM synthesis.
24679493	9	62	theme	prolonged	1318:1326	arg1	survival					1328:1335	prolonged survival	1318:1335	prolonged survival of MSCs	1318:1343	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
24679493	3	63	dep	in	523:524	arg1	vivo					526:529	vivo	526:529	vivo	526:529	VF scars were evaluated with in vivo fluorescence live imaging system (IFLIS), endoscopy, histology, and videokymography (VKG) after eight weeks.
24679493	3	64	dep	imaging	549:555	arg1	IFLIS					565:569	IFLIS	565:569	IFLIS	565:569	VF scars were evaluated with in vivo fluorescence live imaging system (IFLIS), endoscopy, histology, and videokymography (VKG) after eight weeks.
24679493	3	64	dep	imaging	549:555	arg1	system					557:562	system	557:562	in vivo fluorescence live imaging system (IFLIS)	523:570	VF scars were evaluated with in vivo fluorescence live imaging system (IFLIS), endoscopy, histology, and videokymography (VKG) after eight weeks.
24679493	3	65	theme	VF	494:495	arg1	scars					497:501	VF scars	494:501	VF scars	494:501	VF scars were evaluated with in vivo fluorescence live imaging system (IFLIS), endoscopy, histology, and videokymography (VKG) after eight weeks.
24679493	9	66	theme	present	1229:1235	arg1	investigation					1237:1249	the present investigation	1225:1249	the present investigation	1225:1249	The results of the present investigation suggest that SIS-MSCs composite gel is a plausible biomaterial for prolonged survival of MSCs in VFs and promotes scarless VF healing.
26926504	8	0	theme	1.37	1707:1710	arg1	%					1711:1711	%	1711:1711	%	1711:1711	During the 30-day follow-up, NACE occurred in 3.45% (103/2 988) patients, the incidence of MACE, death was 2.17% (65/2 994) and 1.03% (31/3 017), respectively and bleeding events were recorded in 1.37% (41/2 996) patients.
26926504	3	1	theme	cardiac	652:658	arg1	stroke					760:765	stroke	760:765	stroke	760:765	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	1	theme	cardiac	652:658	arg1	MACE					680:683	MACE	680:683	MACE	680:683	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	1	theme	cardiac	652:658	arg1	revascularization					738:754	urgent target vessel revascularization	717:754	urgent target vessel revascularization	717:754	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	1	theme	cardiac	652:658	arg1	death					696:700	all-cause death	686:700	all-cause death	686:700	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	1	theme	cardiac	652:658	arg1	events					672:677	major adverse cardiac or cerebral events	638:677	major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	638:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	1	theme	cardiac	652:658	arg1	reinfarction					703:714	reinfarction	703:714	reinfarction	703:714	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	1	2	theme	Chinese	252:258	arg1	patients					260:267	Chinese patients	252:267	Chinese patients with coronary heart disease (CHD)	252:301	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	6	3	theme	%	1262:1262	arg1	patients					1278:1285	9.7% (316/3 271) and 34.1% (1 115/3 271) patients	1237:1285	9.7% (316/3 271) and 34.1% (1 115/3 271) patients	1237:1285	Radial access was performed in 89.5% (2 879/3 271) patients, and 9.7% (316/3 271) and 34.1% (1 115/3 271) patients also received ticagrelor and tirofiban medication.
26926504	10	4	theme	Bivalirudin	1835:1845	arg1	use					1847:1849	CONCLUSION Peri-PCI Bivalirudin use	1815:1849	CONCLUSION Peri-PCI Bivalirudin use	1815:1849	CONCLUSION Peri-PCI Bivalirudin use is safe and related with low bleeding risk in Chinese CHD patients.
26926504	4	5	theme	enrolled	861:868	arg1	patients					870:877	enrolled patients	861:877	enrolled patients	861:877	RESULTS The mean age of enrolled patients was (65.12±12.44) years old, 27.4%(889/3 244) of them were female.
26926504	3	6	theme	vessel	731:736	arg1	events					672:677	major adverse cardiac or cerebral events	638:677	major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	638:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	6	theme	vessel	731:736	arg1	revascularization					738:754	urgent target vessel revascularization	717:754	urgent target vessel revascularization	717:754	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	5	7	theme	infarction	1076:1085	arg1	Percent					946:952	Percent	946:952	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI)	946:1093	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	8	theme	coronary	964:971	arg1	SCD					982:984	SCD	982:984	SCD	982:984	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	8	theme	coronary	964:971	arg1	disease					973:979	stable coronary disease	957:979	stable coronary disease (SCD)	957:985	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	1	9	from	efficacy	210:217	arg1	patients					260:267	Chinese patients	252:267	Chinese patients with coronary heart disease (CHD)	252:301	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	3	10	theme	urgent	717:722	arg1	events					672:677	major adverse cardiac or cerebral events	638:677	major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	638:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	10	theme	urgent	717:722	arg1	revascularization					738:754	urgent target vessel revascularization	717:754	urgent target vessel revascularization	717:754	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	8	11	theme	%	1561:1561	arg1	patients					1575:1582	3.45% (103/2 988) patients	1557:1582	3.45% (103/2 988) patients	1557:1582	During the 30-day follow-up, NACE occurred in 3.45% (103/2 988) patients, the incidence of MACE, death was 2.17% (65/2 994) and 1.03% (31/3 017), respectively and bleeding events were recorded in 1.37% (41/2 996) patients.
26926504	3	12	theme	primary	562:568	arg1	outcome					570:576	The primary outcome	558:576	The primary outcome	558:576	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	12	theme	primary	562:568	arg1	events					610:615	30-day net adverse clinical events	582:615	30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding)	582:779	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	7	13	theme	050/2	1432:1436	arg1	%					1427:1427	46.3%	1423:1427	46.3% (1 050/2 266)	1423:1441	69.3% (2 266/3 271) patients received post-procedural bivalirudin infusion, in which 46.3% (1 050/2 266) was treated at PCI-does, with a median duration of 2.5(1.0, 4.0) h.
26926504	7	13	theme	050/2	1432:1436	arg1	266					1438:1440	1 050/2 266	1430:1440	1 050/2 266	1430:1440	69.3% (2 266/3 271) patients received post-procedural bivalirudin infusion, in which 46.3% (1 050/2 266) was treated at PCI-does, with a median duration of 2.5(1.0, 4.0) h.
26926504	10	14	from	risk	1889:1892	arg1	patients					1909:1916	Chinese CHD patients	1897:1916	Chinese CHD patients	1897:1916	CONCLUSION Peri-PCI Bivalirudin use is safe and related with low bleeding risk in Chinese CHD patients.
26926504	3	15	from	days	831:834	arg1	outcome					796:802	the secondary outcome	782:802	the secondary outcome	782:802	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	15	from	days	831:834	arg1	thrombosis					814:823	stent thrombosis	808:823	stent thrombosis	808:823	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	0	16	theme	heart	169:173	arg1	disease					175:181	coronary heart disease	160:181	coronary heart disease	160:181	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	3	17	theme	clinical	601:608	arg1	outcome					570:576	The primary outcome	558:576	The primary outcome	558:576	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	17	theme	clinical	601:608	arg1	events					610:615	30-day net adverse clinical events	582:615	30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding)	582:779	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	10	18	theme	bleeding	1880:1887	arg1	risk					1889:1892	low bleeding risk	1876:1892	low bleeding risk in Chinese CHD patients	1876:1916	CONCLUSION Peri-PCI Bivalirudin use is safe and related with low bleeding risk in Chinese CHD patients.
26926504	6	19	theme	Radial	1172:1177	arg1	access					1179:1184	Radial access	1172:1184	Radial access	1172:1184	Radial access was performed in 89.5% (2 879/3 271) patients, and 9.7% (316/3 271) and 34.1% (1 115/3 271) patients also received ticagrelor and tirofiban medication.
26926504	5	20	theme	non-ST	988:993	arg1	elevation					1003:1011	non-ST segment elevation	988:1011	non-ST segment elevation acute coronary syndrome (NSTE-ACS)	988:1046	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	3	21	theme	net	589:591	arg1	outcome					570:576	The primary outcome	558:576	The primary outcome	558:576	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	21	theme	net	589:591	arg1	events					610:615	30-day net adverse clinical events	582:615	30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding)	582:779	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	0	22	theme	bivalirudin	64:74	arg1	efficacy					52:59	efficacy	52:59	efficacy of bivalirudin	52:74	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	1	23	theme	intervention	341:352	arg1	period					359:364	the peri-percutaneous coronary intervention(PCI) period	310:364	the peri-percutaneous coronary intervention(PCI) period	310:364	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	3	24	theme	stent	808:812	arg1	outcome					796:802	the secondary outcome	782:802	the secondary outcome	782:802	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	24	theme	stent	808:812	arg1	thrombosis					814:823	stent thrombosis	808:823	stent thrombosis	808:823	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	10	25	theme	CHD	1905:1907	arg1	patients					1909:1916	Chinese CHD patients	1897:1916	Chinese CHD patients	1897:1916	CONCLUSION Peri-PCI Bivalirudin use is safe and related with low bleeding risk in Chinese CHD patients.
26926504	1	26	theme	peri-percutaneous	314:330	arg1	intervention					341:352	peri-percutaneous coronary intervention	314:352	the peri-percutaneous coronary intervention(PCI) period	310:364	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	1	26	theme	peri-percutaneous	314:330	arg1	PCI					354:356	PCI	354:356	PCI	354:356	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	9	27	theme	stent	1756:1760	arg1	thrombosis					1762:1771	stent thrombosis	1756:1771	stent thrombosis	1756:1771	Four cases (0.13%) of stent thrombosis (3 acute stent thrombosis) were recorded.
26926504	0	28	from	study	43:47	arg1	efficacy					52:59	efficacy	52:59	efficacy of bivalirudin	52:74	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	0	29	theme	coronary	105:112	arg1	intervention					114:125	peri-percutaneous coronary intervention	87:125	peri-percutaneous coronary intervention period	87:132	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	7	30	theme	post-procedural	1376:1390	arg1	infusion					1404:1411	post-procedural bivalirudin infusion	1376:1411	post-procedural bivalirudin infusion	1376:1411	69.3% (2 266/3 271) patients received post-procedural bivalirudin infusion, in which 46.3% (1 050/2 266) was treated at PCI-does, with a median duration of 2.5(1.0, 4.0) h.
26926504	3	31	dep	events	672:677	arg1	stroke					760:765	stroke	760:765	stroke	760:765	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	31	dep	events	672:677	arg1	MACE					680:683	MACE	680:683	MACE	680:683	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	31	dep	events	672:677	arg1	revascularization					738:754	urgent target vessel revascularization	717:754	urgent target vessel revascularization	717:754	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	31	dep	events	672:677	arg1	death					696:700	all-cause death	686:700	all-cause death	686:700	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	31	dep	events	672:677	arg1	events					672:677	major adverse cardiac or cerebral events	638:677	major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	638:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	31	dep	events	672:677	arg1	reinfarction					703:714	reinfarction	703:714	reinfarction	703:714	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	9	32	theme	acute	1776:1780	arg1	cases					1739:1743	Four cases	1734:1743	Four cases (0.13%) of stent thrombosis (3 acute stent thrombosis)	1734:1798	Four cases (0.13%) of stent thrombosis (3 acute stent thrombosis) were recorded.
26926504	9	32	theme	acute	1776:1780	arg1	thrombosis					1788:1797	3 acute stent thrombosis	1774:1797	3 acute stent thrombosis	1774:1797	Four cases (0.13%) of stent thrombosis (3 acute stent thrombosis) were recorded.
26926504	0	33	theme	[A	0:1	arg1	study					43:47	[A large-scale, multicenter, retrospective study	0:47	study	43:47	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	1	34	theme	coronary	274:281	arg1	disease					289:295	coronary heart disease	274:295	coronary heart disease (CHD)	274:301	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	1	34	theme	coronary	274:281	arg1	CHD					298:300	CHD	298:300	CHD	298:300	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	4	35	theme	them	928:931	arg1	%					912:912	27.4%	908:912	27.4%(889/3 244) of them	908:931	RESULTS The mean age of enrolled patients was (65.12±12.44) years old, 27.4%(889/3 244) of them were female.
26926504	4	35	theme	them	928:931	arg1	244					920:922	889/3 244	914:922	889/3 244	914:922	RESULTS The mean age of enrolled patients was (65.12±12.44) years old, 27.4%(889/3 244) of them were female.
26926504	4	35	theme	them	928:931	arg1	them					928:931	them	928:931	them	928:931	RESULTS The mean age of enrolled patients was (65.12±12.44) years old, 27.4%(889/3 244) of them were female.
26926504	6	36	theme	%	1207:1207	arg1	patients					1223:1230	89.5% (2 879/3 271) patients	1203:1230	89.5% (2 879/3 271) patients	1203:1230	Radial access was performed in 89.5% (2 879/3 271) patients, and 9.7% (316/3 271) and 34.1% (1 115/3 271) patients also received ticagrelor and tirofiban medication.
26926504	5	37	theme	ST	1052:1053	arg1	STEMI					1088:1092	STEMI	1088:1092	STEMI	1088:1092	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	37	theme	ST	1052:1053	arg1	infarction					1076:1085	ST elevation myocardial infarction	1052:1085	ST elevation myocardial infarction (STEMI)	1052:1093	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	38	theme	coronary	1019:1026	arg1	NSTE-ACS					1038:1045	NSTE-ACS	1038:1045	NSTE-ACS	1038:1045	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	38	theme	coronary	1019:1026	arg1	syndrome					1028:1035	non-ST segment elevation acute coronary syndrome	988:1035	non-ST segment elevation acute coronary syndrome (NSTE-ACS)	988:1046	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	1	39	with	patients	260:267	arg1	disease					289:295	coronary heart disease	274:295	coronary heart disease (CHD)	274:301	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	1	39	with	patients	260:267	arg1	CHD					298:300	CHD	298:300	CHD	298:300	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	2	40	theme	bivalirudin	447:457	arg1	treatment					459:467	periprocedural bivalirudin treatment	432:467	periprocedural bivalirudin treatment between July 2013 and October 2015 from 88 centers of China	432:527	METHODS A total of 3 271 patients who underwent PCI and received periprocedural bivalirudin treatment between July 2013 and October 2015 from 88 centers of China were involved in this study.
26926504	4	41	dep	RESULTS	837:843	arg1	age					854:856	The mean age	845:856	The mean age of enrolled patients	845:877	RESULTS The mean age of enrolled patients was (65.12±12.44) years old, 27.4%(889/3 244) of them were female.
26926504	4	41	dep	RESULTS	837:843	arg1	old					903:905	old	903:905	old	903:905	RESULTS The mean age of enrolled patients was (65.12±12.44) years old, 27.4%(889/3 244) of them were female.
26926504	2	42	dep	July	477:480	arg1	2013					482:485	2013	482:485	2013	482:485	METHODS A total of 3 271 patients who underwent PCI and received periprocedural bivalirudin treatment between July 2013 and October 2015 from 88 centers of China were involved in this study.
26926504	3	43	theme	adverse	644:650	arg1	stroke					760:765	stroke	760:765	stroke	760:765	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	43	theme	adverse	644:650	arg1	MACE					680:683	MACE	680:683	MACE	680:683	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	43	theme	adverse	644:650	arg1	revascularization					738:754	urgent target vessel revascularization	717:754	urgent target vessel revascularization	717:754	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	43	theme	adverse	644:650	arg1	death					696:700	all-cause death	686:700	all-cause death	686:700	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	43	theme	adverse	644:650	arg1	events					672:677	major adverse cardiac or cerebral events	638:677	major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	638:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	43	theme	adverse	644:650	arg1	reinfarction					703:714	reinfarction	703:714	reinfarction	703:714	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	1	44	dep	efficacy	210:217	arg1	the					206:208	the	206:208	the	206:208	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	6	45	theme	%	1240:1240	arg1	patients					1278:1285	9.7% (316/3 271) and 34.1% (1 115/3 271) patients	1237:1285	9.7% (316/3 271) and 34.1% (1 115/3 271) patients	1237:1285	Radial access was performed in 89.5% (2 879/3 271) patients, and 9.7% (316/3 271) and 34.1% (1 115/3 271) patients also received ticagrelor and tirofiban medication.
26926504	3	46	dep	events	610:615	arg1	NACE					618:621	NACE	618:621	NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding	618:778	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	7	47	theme	h	1508:1508	arg1	duration					1482:1489	a median duration	1473:1489	a median duration of 2.5(1.0, 4.0) h	1473:1508	69.3% (2 266/3 271) patients received post-procedural bivalirudin infusion, in which 46.3% (1 050/2 266) was treated at PCI-does, with a median duration of 2.5(1.0, 4.0) h.
26926504	8	48	theme	bleeding	1674:1681	arg1	events					1683:1688	bleeding events	1674:1688	bleeding events	1674:1688	During the 30-day follow-up, NACE occurred in 3.45% (103/2 988) patients, the incidence of MACE, death was 2.17% (65/2 994) and 1.03% (31/3 017), respectively and bleeding events were recorded in 1.37% (41/2 996) patients.
26926504	5	49	theme	636/3	1147:1151	arg1	248					1153:1155	1 636/3 248	1145:1155	1 636/3 248	1145:1155	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	49	theme	636/3	1147:1151	arg1	%					1143:1143	50.4%	1139:1143	50.4%(1 636/3 248)	1139:1156	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	3	50	dep	NACE	618:621	arg1	bleeding					771:778	bleeding	771:778	bleeding	771:778	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	50	dep	NACE	618:621	arg1	events					672:677	major adverse cardiac or cerebral events	638:677	major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	638:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	50	dep	NACE	618:621	arg1	composite					625:633	a composite	623:633	a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	623:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	7	51	theme	median	1475:1480	arg1	duration					1482:1489	a median duration	1473:1489	a median duration of 2.5(1.0, 4.0) h	1473:1508	69.3% (2 266/3 271) patients received post-procedural bivalirudin infusion, in which 46.3% (1 050/2 266) was treated at PCI-does, with a median duration of 2.5(1.0, 4.0) h.
26926504	3	52	theme	events	672:677	arg1	events					672:677	major adverse cardiac or cerebral events	638:677	major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	638:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	52	theme	events	672:677	arg1	composite					625:633	a composite	623:633	a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	623:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	5	53	theme	myocardial	1065:1074	arg1	STEMI					1088:1092	STEMI	1088:1092	STEMI	1088:1092	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	53	theme	myocardial	1065:1074	arg1	infarction					1076:1085	ST elevation myocardial infarction	1052:1085	ST elevation myocardial infarction (STEMI)	1052:1093	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	54	theme	segment	995:1001	arg1	elevation					1003:1011	non-ST segment elevation	988:1011	non-ST segment elevation acute coronary syndrome (NSTE-ACS)	988:1046	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	10	55	with	safe	1854:1857	arg1	risk					1889:1892	low bleeding risk	1876:1892	low bleeding risk in Chinese CHD patients	1876:1916	CONCLUSION Peri-PCI Bivalirudin use is safe and related with low bleeding risk in Chinese CHD patients.
26926504	8	56	theme	%	1711:1711	arg1	41/2					1714:1717	41/2 996	1714:1721	41/2 996	1714:1721	During the 30-day follow-up, NACE occurred in 3.45% (103/2 988) patients, the incidence of MACE, death was 2.17% (65/2 994) and 1.03% (31/3 017), respectively and bleeding events were recorded in 1.37% (41/2 996) patients.
26926504	8	56	theme	%	1711:1711	arg1	patients					1724:1731	1.37% (41/2 996) patients	1707:1731	1.37% (41/2 996) patients	1707:1731	During the 30-day follow-up, NACE occurred in 3.45% (103/2 988) patients, the incidence of MACE, death was 2.17% (65/2 994) and 1.03% (31/3 017), respectively and bleeding events were recorded in 1.37% (41/2 996) patients.
26926504	3	57	theme	all-cause	686:694	arg1	death					696:700	all-cause death	686:700	all-cause death	686:700	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	57	theme	all-cause	686:694	arg1	events					672:677	major adverse cardiac or cerebral events	638:677	major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	638:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	8	58	theme	30-day	1522:1527	arg1	follow-up					1529:1537	the 30-day follow-up	1518:1537	the 30-day follow-up	1518:1537	During the 30-day follow-up, NACE occurred in 3.45% (103/2 988) patients, the incidence of MACE, death was 2.17% (65/2 994) and 1.03% (31/3 017), respectively and bleeding events were recorded in 1.37% (41/2 996) patients.
26926504	2	59	theme	periprocedural	432:445	arg1	treatment					459:467	periprocedural bivalirudin treatment	432:467	periprocedural bivalirudin treatment between July 2013 and October 2015 from 88 centers of China	432:527	METHODS A total of 3 271 patients who underwent PCI and received periprocedural bivalirudin treatment between July 2013 and October 2015 from 88 centers of China were involved in this study.
26926504	5	60	theme	elevation	1003:1011	arg1	NSTE-ACS					1038:1045	NSTE-ACS	1038:1045	NSTE-ACS	1038:1045	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	60	theme	elevation	1003:1011	arg1	syndrome					1028:1035	non-ST segment elevation acute coronary syndrome	988:1035	non-ST segment elevation acute coronary syndrome (NSTE-ACS)	988:1046	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	7	61	theme	%	1342:1342	arg1	patients					1358:1365	69.3% (2 266/3 271) patients	1338:1365	69.3% (2 266/3 271) patients	1338:1365	69.3% (2 266/3 271) patients received post-procedural bivalirudin infusion, in which 46.3% (1 050/2 266) was treated at PCI-does, with a median duration of 2.5(1.0, 4.0) h.
26926504	4	62	theme	mean	849:852	arg1	age					854:856	The mean age	845:856	The mean age of enrolled patients	845:877	RESULTS The mean age of enrolled patients was (65.12±12.44) years old, 27.4%(889/3 244) of them were female.
26926504	4	62	theme	mean	849:852	arg1	old					903:905	old	903:905	old	903:905	RESULTS The mean age of enrolled patients was (65.12±12.44) years old, 27.4%(889/3 244) of them were female.
26926504	5	63	theme	stable	957:962	arg1	SCD					982:984	SCD	982:984	SCD	982:984	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	63	theme	stable	957:962	arg1	disease					973:979	stable coronary disease	957:979	stable coronary disease (SCD)	957:985	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	0	64	theme	coronary	160:167	arg1	disease					175:181	coronary heart disease	160:181	coronary heart disease	160:181	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	5	65	theme	disease	973:979	arg1	Percent					946:952	Percent	946:952	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI)	946:1093	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	6	66	theme	ticagrelor	1301:1310	arg1	medication					1326:1335	ticagrelor and tirofiban medication	1301:1335	ticagrelor and tirofiban medication	1301:1335	Radial access was performed in 89.5% (2 879/3 271) patients, and 9.7% (316/3 271) and 34.1% (1 115/3 271) patients also received ticagrelor and tirofiban medication.
26926504	0	67	with	patients	146:153	arg1	disease					175:181	coronary heart disease	160:181	coronary heart disease	160:181	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	3	68	theme	target	724:729	arg1	events					672:677	major adverse cardiac or cerebral events	638:677	major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	638:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	68	theme	target	724:729	arg1	revascularization					738:754	urgent target vessel revascularization	717:754	urgent target vessel revascularization	717:754	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	1	69	from	safety	223:228	arg1	patients					260:267	Chinese patients	252:267	Chinese patients with coronary heart disease (CHD)	252:301	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	3	70	theme	adverse	593:599	arg1	outcome					570:576	The primary outcome	558:576	The primary outcome	558:576	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	70	theme	adverse	593:599	arg1	events					610:615	30-day net adverse clinical events	582:615	30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding)	582:779	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	2	71	from	centers	512:518	arg1	treatment					459:467	periprocedural bivalirudin treatment	432:467	periprocedural bivalirudin treatment between July 2013 and October 2015 from 88 centers of China	432:527	METHODS A total of 3 271 patients who underwent PCI and received periprocedural bivalirudin treatment between July 2013 and October 2015 from 88 centers of China were involved in this study.
26926504	3	72	theme	30-day	582:587	arg1	outcome					570:576	The primary outcome	558:576	The primary outcome	558:576	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	72	theme	30-day	582:587	arg1	events					610:615	30-day net adverse clinical events	582:615	30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding)	582:779	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	73	theme	secondary	786:794	arg1	outcome					796:802	the secondary outcome	782:802	the secondary outcome	782:802	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	73	theme	secondary	786:794	arg1	thrombosis					814:823	stent thrombosis	808:823	stent thrombosis	808:823	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	10	74	theme	low	1876:1878	arg1	risk					1889:1892	low bleeding risk	1876:1892	low bleeding risk in Chinese CHD patients	1876:1916	CONCLUSION Peri-PCI Bivalirudin use is safe and related with low bleeding risk in Chinese CHD patients.
26926504	10	75	theme	Chinese	1897:1903	arg1	patients					1909:1916	Chinese CHD patients	1897:1916	Chinese CHD patients	1897:1916	CONCLUSION Peri-PCI Bivalirudin use is safe and related with low bleeding risk in Chinese CHD patients.
26926504	1	76	theme	coronary	332:339	arg1	intervention					341:352	peri-percutaneous coronary intervention	314:352	the peri-percutaneous coronary intervention(PCI) period	310:364	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	1	76	theme	coronary	332:339	arg1	PCI					354:356	PCI	354:356	PCI	354:356	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	10	77	theme	Peri-PCI	1826:1833	arg1	use					1847:1849	CONCLUSION Peri-PCI Bivalirudin use	1815:1849	CONCLUSION Peri-PCI Bivalirudin use	1815:1849	CONCLUSION Peri-PCI Bivalirudin use is safe and related with low bleeding risk in Chinese CHD patients.
26926504	0	78	theme	peri-percutaneous	87:103	arg1	intervention					114:125	peri-percutaneous coronary intervention	87:125	peri-percutaneous coronary intervention period	87:132	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	5	79	theme	acute	1013:1017	arg1	NSTE-ACS					1038:1045	NSTE-ACS	1038:1045	NSTE-ACS	1038:1045	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	79	theme	acute	1013:1017	arg1	syndrome					1028:1035	non-ST segment elevation acute coronary syndrome	988:1035	non-ST segment elevation acute coronary syndrome (NSTE-ACS)	988:1046	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	4	80	theme	patients	870:877	arg1	age					854:856	The mean age	845:856	The mean age of enrolled patients	845:877	RESULTS The mean age of enrolled patients was (65.12±12.44) years old, 27.4%(889/3 244) of them were female.
26926504	4	80	theme	patients	870:877	arg1	old					903:905	old	903:905	old	903:905	RESULTS The mean age of enrolled patients was (65.12±12.44) years old, 27.4%(889/3 244) of them were female.
26926504	9	81	theme	thrombosis	1762:1771	arg1	cases					1739:1743	Four cases	1734:1743	Four cases (0.13%) of stent thrombosis (3 acute stent thrombosis)	1734:1798	Four cases (0.13%) of stent thrombosis (3 acute stent thrombosis) were recorded.
26926504	9	81	theme	thrombosis	1762:1771	arg1	%					1750:1750	0.13%	1746:1750	0.13%	1746:1750	Four cases (0.13%) of stent thrombosis (3 acute stent thrombosis) were recorded.
26926504	9	81	theme	thrombosis	1762:1771	arg1	thrombosis					1788:1797	3 acute stent thrombosis	1774:1797	3 acute stent thrombosis	1774:1797	Four cases (0.13%) of stent thrombosis (3 acute stent thrombosis) were recorded.
26926504	0	82	theme	intervention	114:125	arg1	period					127:132	peri-percutaneous coronary intervention period	87:132	peri-percutaneous coronary intervention period	87:132	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	0	83	theme	large-scale	3:13	arg1	study					43:47	[A large-scale, multicenter, retrospective study	0:47	study	43:47	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	7	84	theme	bivalirudin	1392:1402	arg1	infusion					1404:1411	post-procedural bivalirudin infusion	1376:1411	post-procedural bivalirudin infusion	1376:1411	69.3% (2 266/3 271) patients received post-procedural bivalirudin infusion, in which 46.3% (1 050/2 266) was treated at PCI-does, with a median duration of 2.5(1.0, 4.0) h.
26926504	2	85	theme	China	523:527	arg1	centers					512:518	88 centers	509:518	88 centers of China	509:527	METHODS A total of 3 271 patients who underwent PCI and received periprocedural bivalirudin treatment between July 2013 and October 2015 from 88 centers of China were involved in this study.
26926504	9	86	theme	stent	1782:1786	arg1	cases					1739:1743	Four cases	1734:1743	Four cases (0.13%) of stent thrombosis (3 acute stent thrombosis)	1734:1798	Four cases (0.13%) of stent thrombosis (3 acute stent thrombosis) were recorded.
26926504	9	86	theme	stent	1782:1786	arg1	thrombosis					1788:1797	3 acute stent thrombosis	1774:1797	3 acute stent thrombosis	1774:1797	Four cases (0.13%) of stent thrombosis (3 acute stent thrombosis) were recorded.
26926504	10	87	theme	CONCLUSION	1815:1824	arg1	use					1847:1849	CONCLUSION Peri-PCI Bivalirudin use	1815:1849	CONCLUSION Peri-PCI Bivalirudin use	1815:1849	CONCLUSION Peri-PCI Bivalirudin use is safe and related with low bleeding risk in Chinese CHD patients.
26926504	1	88	theme	heart	283:287	arg1	disease					289:295	coronary heart disease	274:295	coronary heart disease (CHD)	274:301	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	1	88	theme	heart	283:287	arg1	CHD					298:300	CHD	298:300	CHD	298:300	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	6	89	theme	tirofiban	1316:1324	arg1	medication					1326:1335	ticagrelor and tirofiban medication	1301:1335	ticagrelor and tirofiban medication	1301:1335	Radial access was performed in 89.5% (2 879/3 271) patients, and 9.7% (316/3 271) and 34.1% (1 115/3 271) patients also received ticagrelor and tirofiban medication.
26926504	2	90	theme	patients	392:399	arg1	total					377:381	A total	375:381	A total of 3 271 patients who underwent PCI and received periprocedural bivalirudin treatment between July 2013 and October 2015 from 88 centers of China	375:527	METHODS A total of 3 271 patients who underwent PCI and received periprocedural bivalirudin treatment between July 2013 and October 2015 from 88 centers of China were involved in this study.
26926504	0	91	theme	retrospective	29:41	arg1	study					43:47	[A large-scale, multicenter, retrospective study	0:47	study	43:47	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	5	92	theme	elevation	1055:1063	arg1	STEMI					1088:1092	STEMI	1088:1092	STEMI	1088:1092	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	5	92	theme	elevation	1055:1063	arg1	infarction					1076:1085	ST elevation myocardial infarction	1052:1085	ST elevation myocardial infarction (STEMI)	1052:1093	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	1	93	theme	bivalirudin	233:243	arg1	use					245:247	bivalirudin use	233:247	bivalirudin use	233:247	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	8	94	theme	MACE	1602:1605	arg1	%					1622:1622	2.17%	1618:1622	2.17% (65/2 994)	1618:1633	During the 30-day follow-up, NACE occurred in 3.45% (103/2 988) patients, the incidence of MACE, death was 2.17% (65/2 994) and 1.03% (31/3 017), respectively and bleeding events were recorded in 1.37% (41/2 996) patients.
26926504	8	94	theme	MACE	1602:1605	arg1	incidence					1589:1597	the incidence	1585:1597	the incidence of MACE, death	1585:1612	During the 30-day follow-up, NACE occurred in 3.45% (103/2 988) patients, the incidence of MACE, death was 2.17% (65/2 994) and 1.03% (31/3 017), respectively and bleeding events were recorded in 1.37% (41/2 996) patients.
26926504	5	95	theme	syndrome	1028:1035	arg1	Percent					946:952	Percent	946:952	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI)	946:1093	Percent of stable coronary disease (SCD), non-ST segment elevation acute coronary syndrome (NSTE-ACS) and ST elevation myocardial infarction (STEMI) was 5.0%(162/3 248), 44.6%(1 450/3 248) and 50.4%(1 636/3 248) respectively.
26926504	0	96	theme	Chinese	138:144	arg1	patients					146:153	Chinese patients	138:153	Chinese patients with coronary heart disease	138:181	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	3	97	theme	major	638:642	arg1	stroke					760:765	stroke	760:765	stroke	760:765	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	97	theme	major	638:642	arg1	MACE					680:683	MACE	680:683	MACE	680:683	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	97	theme	major	638:642	arg1	revascularization					738:754	urgent target vessel revascularization	717:754	urgent target vessel revascularization	717:754	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	97	theme	major	638:642	arg1	death					696:700	all-cause death	686:700	all-cause death	686:700	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	97	theme	major	638:642	arg1	events					672:677	major adverse cardiac or cerebral events	638:677	major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	638:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	97	theme	major	638:642	arg1	reinfarction					703:714	reinfarction	703:714	reinfarction	703:714	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	2	98	dep	METHODS	367:373	arg1	involved					534:541	involved	534:541	were involved in this study	529:555	METHODS A total of 3 271 patients who underwent PCI and received periprocedural bivalirudin treatment between July 2013 and October 2015 from 88 centers of China were involved in this study.
26926504	0	99	dep	large-scale	3:13	arg1	multicenter					16:26	multicenter	16:26	multicenter	16:26	[A large-scale, multicenter, retrospective study on efficacy of bivalirudin use during peri-percutaneous coronary intervention period for Chinese patients with coronary heart disease].
26926504	3	100	theme	cerebral	663:670	arg1	stroke					760:765	stroke	760:765	stroke	760:765	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	100	theme	cerebral	663:670	arg1	MACE					680:683	MACE	680:683	MACE	680:683	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	100	theme	cerebral	663:670	arg1	revascularization					738:754	urgent target vessel revascularization	717:754	urgent target vessel revascularization	717:754	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	100	theme	cerebral	663:670	arg1	death					696:700	all-cause death	686:700	all-cause death	686:700	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	100	theme	cerebral	663:670	arg1	events					672:677	major adverse cardiac or cerebral events	638:677	major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke)	638:766	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	3	100	theme	cerebral	663:670	arg1	reinfarction					703:714	reinfarction	703:714	reinfarction	703:714	The primary outcome was 30-day net adverse clinical events (NACE a composite of major adverse cardiac or cerebral events (MACE, all-cause death, reinfarction, urgent target vessel revascularization, or stroke) or bleeding), the secondary outcome was stent thrombosis at 30 days.
26926504	1	101	theme	use	245:247	arg1	safety					223:228	safety	223:228	safety	223:228	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26926504	1	101	theme	use	245:247	arg1	efficacy					210:217	efficacy	210:217	efficacy	210:217	OBJECTIVE To observe the efficacy and safety of bivalirudin use in Chinese patients with coronary heart disease (CHD) during the peri-percutaneous coronary intervention(PCI) period.
26851843	15	0	theme	kernel	2472:2477	arg1	fraction					2479:2486	kernel fraction	2472:2486	kernel fraction	2472:2486	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed.
26851843	8	1	dep	in	1356:1357	arg1	situ					1359:1362	situ	1359:1362	situ	1359:1362	The ruminal in situ DM disappearance of unfermented kernels increased with the reduction in particle size of corn kernels.
26851843	2	2	theme	breakage	321:328	arg1	degree					304:309	degree	304:309	degree of kernel breakage in WPCS	304:336	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	14	3	theme	4.75-mm	2338:2344	arg1	screen					2346:2351	the 4.75-mm screen	2334:2351	the 4.75-mm screen	2334:2351	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor.
26851843	10	4	theme	settings	1787:1794	arg1	length					1740:1745	varied theoretical length	1721:1745	varied theoretical length of cut settings and processor types and settings	1721:1794	Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings were also evaluated.
26851843	4	5	theme	kernel	748:753	arg1	fraction					755:762	the kernel fraction	744:762	the kernel fraction	744:762	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	12	6	theme	stover	1998:2003	arg1	fractions					2005:2013	the kernel and stover fractions	1983:2013	the kernel and stover fractions	1983:2013	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	8	7	theme	kernels	1458:1464	arg1	size					1445:1448	particle size	1436:1448	particle size of corn kernels	1436:1464	The ruminal in situ DM disappearance of unfermented kernels increased with the reduction in particle size of corn kernels.
26851843	6	8	theme	ruminal	1193:1199	arg1	GMPS					1020:1023	GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well	1020:1188	GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities	1020:1239	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	6	9	dep	GMPS	1020:1023	arg1	using					1071:1075	using	1071:1075	using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan	1071:1179	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	1	10	theme	starch	128:133	arg1	digestibility					135:147	starch digestibility	128:147	starch digestibility	128:147	Kernel processing increases starch digestibility in whole-plant corn silage (WPCS).
26851843	12	11	theme	kernel	1987:1992	arg1	fractions					2005:2013	the kernel and stover fractions	1983:2013	the kernel and stover fractions	1983:2013	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	14	12	theme	surface	2289:2295	arg1	area					2297:2300	surface area	2289:2300	surface area	2289:2300	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor.
26851843	9	13	theme	lowest	1494:1499	arg1	disappearance					1512:1524	the lowest ruminal DM disappearance	1490:1524	the lowest ruminal DM disappearance for all time points	1490:1544	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	5	14	dep	whole	889:893	arg1	WH					896:897	WH	896:897	WH	896:897	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production were kept whole (WH) or manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively).
26851843	2	15	from	breakage	321:328	arg1	WPCS					333:336	WPCS	333:336	WPCS	333:336	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	9	16	theme	DM	1509:1510	arg1	disappearance					1512:1524	the lowest ruminal DM disappearance	1490:1524	the lowest ruminal DM disappearance for all time points	1490:1544	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	4	17	theme	varied	602:607	arg1	sizes					618:622	varied particle sizes	602:622	varied particle sizes	602:622	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	14	18	from	GMPS	2283:2286	arg1	CSPS					2241:2244	CSPS	2241:2244	CSPS from WPCS (n=80) and kernel fraction GMPS	2241:2286	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor.
26851843	14	18	from	GMPS	2283:2286	arg1	area					2297:2300	surface area	2289:2300	surface area	2289:2300	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor.
26851843	14	18	from	GMPS	2283:2286	arg1	proportion					2307:2316	proportion	2307:2316	proportion passing through the 4.75-mm screen	2307:2351	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor.
26851843	16	19	theme	hydrodynamic	2553:2564	arg1	separation					2566:2575	hydrodynamic separation	2553:2575	hydrodynamic separation	2553:2575	These findings suggest that hydrodynamic separation and dry sieving of the kernel fraction may provide a better assessment of kernel breakage in WPCS than CSPS.
26851843	2	20	from	WPCS	333:336	arg1	degree					304:309	degree	304:309	degree of kernel breakage in WPCS	304:336	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	14	21	theme	kernel	2267:2272	arg1	GMPS					2283:2286	kernel fraction GMPS	2267:2286	kernel fraction GMPS	2267:2286	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor.
26851843	6	22	theme	size	1053:1056	arg1	distribution					1058:1069	particle size distribution	1044:1069	particle size distribution	1044:1069	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	9	23	theme	time	1534:1537	arg1	points					1539:1544	all time points	1530:1544	all time points	1530:1544	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	8	24	theme	ruminal	1348:1354	arg1	disappearance					1367:1379	The ruminal in situ DM disappearance	1344:1379	The ruminal in situ DM disappearance of unfermented kernels	1344:1402	The ruminal in situ DM disappearance of unfermented kernels increased with the reduction in particle size of corn kernels.
26851843	13	25	from	h	2138:2138	arg1	dry					2165:2167	dry	2165:2167	dry	2165:2167	After separation, the kernel fraction was redried at 60°C for 48 h in a forced-air oven and dry sieved to determine GMPS and surface area.
26851843	13	25	from	h	2138:2138	arg1	oven					2156:2159	a forced-air oven	2143:2159	a forced-air oven	2143:2159	After separation, the kernel fraction was redried at 60°C for 48 h in a forced-air oven and dry sieved to determine GMPS and surface area.
26851843	12	26	from	duplicate	1912:1920	arg1	1					1925:1925	1	1925:1925	1	1925:1925	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	12	26	from	duplicate	1912:1920	arg1	samples					1940:1946	the split samples	1930:1946	the split samples	1930:1946	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	12	27	dep	determined	1898:1907	arg1	whereas					1949:1955	whereas	1949:1955	whereas	1949:1955	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	6	28	theme	DM	1221:1222	arg1	digestibilities					1225:1239	situ dry matter (DM) digestibilities	1204:1239	situ dry matter (DM) digestibilities	1204:1239	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	10	29	from	studies	1700:1706	arg1	Samples					1670:1676	Samples	1670:1676	Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings	1670:1794	Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings were also evaluated.
26851843	8	30	theme	particle	1436:1443	arg1	size					1445:1448	particle size	1436:1448	particle size of corn kernels	1436:1464	The ruminal in situ DM disappearance of unfermented kernels increased with the reduction in particle size of corn kernels.
26851843	15	31	theme	Strong	2364:2369	arg1	relationships					2381:2393	Strong quadratic relationships	2364:2393	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area	2364:2508	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed.
26851843	9	32	theme	DM	1559:1560	arg1	disappearance					1562:1574	maximum DM disappearance	1551:1574	maximum DM disappearance of 6.9% at 24 h	1551:1590	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	6	33	with	pan	1177:1179	arg1	apertures					1106:1114	nominal square apertures	1091:1114	nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm	1091:1171	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	4	34	dep	evaluate	530:537	arg1	1					524:524	1	524:524	1	524:524	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	4	34	dep	evaluate	530:537	arg1	evaluate					693:700	evaluate	693:700	to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS	690:770	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	6	35	theme	matter	1213:1218	arg1	digestibilities					1225:1239	situ dry matter (DM) digestibilities	1204:1239	situ dry matter (DM) digestibilities	1204:1239	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	1	36	theme	whole-plant	152:162	arg1	silage					169:174	whole-plant corn silage	152:174	whole-plant corn silage (WPCS)	152:181	Kernel processing increases starch digestibility in whole-plant corn silage (WPCS).
26851843	1	36	theme	whole-plant	152:162	arg1	WPCS					177:180	WPCS	177:180	WPCS	177:180	Kernel processing increases starch digestibility in whole-plant corn silage (WPCS).
26851843	4	37	dep	method	642:647	arg1	measure					652:658	measure	652:658	to measure GMPS in WPCS kernels	649:679	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	8	38	theme	unfermented	1384:1394	arg1	kernels					1396:1402	unfermented kernels	1384:1402	unfermented kernels	1384:1402	The ruminal in situ DM disappearance of unfermented kernels increased with the reduction in particle size of corn kernels.
26851843	12	39	theme	samples	1940:1946	arg1	1					1925:1925	1	1925:1925	1	1925:1925	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	12	39	theme	samples	1940:1946	arg1	samples					1940:1946	the split samples	1930:1946	the split samples	1930:1946	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	9	40	theme	%	1582:1582	arg1	disappearance					1562:1574	maximum DM disappearance	1551:1574	maximum DM disappearance of 6.9% at 24 h	1551:1590	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	0	41	theme	corn	87:90	arg1	silage					92:97	whole-plant corn silage	75:97	whole-plant corn silage	75:97	Relationship between processing score and kernel-fraction particle size in whole-plant corn silage.
26851843	6	42	dep	using	1071:1075	arg1	area					1034:1037	surface area	1026:1037	surface area	1026:1037	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	13	43	theme	forced-air	2145:2154	arg1	oven					2156:2159	a forced-air oven	2143:2159	a forced-air oven	2143:2159	After separation, the kernel fraction was redried at 60°C for 48 h in a forced-air oven and dry sieved to determine GMPS and surface area.
26851843	0	44	from	Relationship	0:11	arg1	silage					92:97	whole-plant corn silage	75:97	whole-plant corn silage	75:97	Relationship between processing score and kernel-fraction particle size in whole-plant corn silage.
26851843	6	45	theme	particle	1272:1279	arg1	treatment					1288:1296	each kernel particle number treatment	1260:1296	each kernel particle number treatment	1260:1296	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	5	46	theme	corn	830:833	arg1	hybrids					835:841	110 corn hybrids	826:841	110 corn hybrids commonly used for silage production	826:877	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production were kept whole (WH) or manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively).
26851843	5	47	theme	unfermented	794:804	arg1	kernels					813:819	unfermented, dried kernels	794:819	unfermented, dried kernels	794:819	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production were kept whole (WH) or manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively).
26851843	10	48	theme	cut	1750:1752	arg1	settings					1754:1761	cut settings	1750:1761	cut settings	1750:1761	Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings were also evaluated.
26851843	16	49	theme	breakage	2658:2665	arg1	assessment					2637:2646	a better assessment	2628:2646	a better assessment of kernel breakage in WPCS than CSPS	2628:2683	These findings suggest that hydrodynamic separation and dry sieving of the kernel fraction may provide a better assessment of kernel breakage in WPCS than CSPS.
26851843	12	50	theme	split	1934:1938	arg1	samples					1940:1946	the split samples	1930:1946	the split samples	1930:1946	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	9	51	theme	greatest	1600:1607	arg1	disappearance					1609:1621	the greatest disappearance	1596:1621	the greatest disappearance	1596:1621	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	1	52	theme	Kernel	100:105	arg1	processing					107:116	Kernel processing	100:116	Kernel processing	100:116	Kernel processing increases starch digestibility in whole-plant corn silage (WPCS).
26851843	15	53	theme	4.75-mm	2453:2459	arg1	screen					2461:2466	the 4.75-mm screen	2449:2466	the 4.75-mm screen	2449:2466	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed.
26851843	10	54	theme	types	1777:1781	arg1	length					1740:1745	varied theoretical length	1721:1745	varied theoretical length of cut settings and processor types and settings	1721:1794	Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings were also evaluated.
26851843	0	55	theme	processing	21:30	arg1	score					32:36	processing score	21:36	processing score	21:36	Relationship between processing score and kernel-fraction particle size in whole-plant corn silage.
26851843	12	56	theme	other	1964:1968	arg1	sample					1976:1981	the other split sample	1960:1981	the other split sample	1960:1981	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	2	57	theme	Corn	184:187	arg1	score					207:211	Corn silage processing score	184:211	Corn silage processing score (CSPS)	184:218	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	2	57	theme	Corn	184:187	arg1	percentage					225:234	the percentage	221:234	the percentage of starch passing through a 4.75-mm sieve	221:276	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	2	57	theme	Corn	184:187	arg1	CSPS					214:217	CSPS	214:217	CSPS	214:217	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	3	58	theme	kernel-fraction	395:409	arg1	GMPS					382:385	GMPS	382:385	GMPS	382:385	However, the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve has not been well described.
26851843	3	58	theme	kernel-fraction	395:409	arg1	size					376:379	the geometric mean particle size	348:379	the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve	348:447	However, the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve has not been well described.
26851843	15	59	theme	fraction	2424:2431	arg1	area					2505:2508	surface area	2497:2508	surface area	2497:2508	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed.
26851843	15	59	theme	fraction	2424:2431	arg1	proportion					2403:2412	proportion	2403:2412	proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS	2403:2491	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed.
26851843	3	60	theme	mean	362:365	arg1	GMPS					382:385	GMPS	382:385	GMPS	382:385	However, the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve has not been well described.
26851843	3	60	theme	mean	362:365	arg1	size					376:379	the geometric mean particle size	348:379	the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve	348:447	However, the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve has not been well described.
26851843	14	61	from	WPCS	2251:2254	arg1	CSPS					2241:2244	CSPS	2241:2244	CSPS from WPCS (n=80) and kernel fraction GMPS	2241:2286	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor.
26851843	14	61	from	WPCS	2251:2254	arg1	area					2297:2300	surface area	2289:2300	surface area	2289:2300	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor.
26851843	14	61	from	WPCS	2251:2254	arg1	proportion					2307:2316	proportion	2307:2316	proportion passing through the 4.75-mm screen	2307:2351	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor.
26851843	2	62	theme	kernel	314:319	arg1	breakage					321:328	kernel breakage	314:328	kernel breakage in WPCS	314:336	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	0	63	theme	particle	58:65	arg1	size					67:70	kernel-fraction particle size	42:70	kernel-fraction particle size	42:70	Relationship between processing score and kernel-fraction particle size in whole-plant corn silage.
26851843	16	64	from	assessment	2637:2646	arg1	WPCS					2670:2673	WPCS	2670:2673	WPCS than CSPS	2670:2683	These findings suggest that hydrodynamic separation and dry sieving of the kernel fraction may provide a better assessment of kernel breakage in WPCS than CSPS.
26851843	8	65	theme	DM	1364:1365	arg1	disappearance					1367:1379	The ruminal in situ DM disappearance	1344:1379	The ruminal in situ DM disappearance of unfermented kernels	1344:1402	The ruminal in situ DM disappearance of unfermented kernels increased with the reduction in particle size of corn kernels.
26851843	6	66	theme	situ	1204:1207	arg1	digestibilities					1225:1239	situ dry matter (DM) digestibilities	1204:1239	situ dry matter (DM) digestibilities	1204:1239	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	15	67	dep	fraction	2424:2431	arg1	GMPS					2488:2491	GMPS	2488:2491	GMPS	2488:2491	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed.
26851843	2	68	used	used	289:292	arg2	CSPS					214:217	CSPS	214:217	CSPS	214:217	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	2	68	used	used	289:292	arg2	score					207:211	Corn silage processing score	184:211	Corn silage processing score (CSPS)	184:218	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	2	68	used	used	289:292	arg2	percentage					225:234	the percentage	221:234	the percentage of starch passing through a 4.75-mm sieve	221:276	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	2	69	theme	processing	196:205	arg1	score					207:211	Corn silage processing score	184:211	Corn silage processing score (CSPS)	184:218	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	2	69	theme	processing	196:205	arg1	percentage					225:234	the percentage	221:234	the percentage of starch passing through a 4.75-mm sieve	221:276	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	2	69	theme	processing	196:205	arg1	CSPS					214:217	CSPS	214:217	CSPS	214:217	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	4	70	theme	particle	539:546	arg1	distribution					553:564	particle size distribution	539:564	particle size distribution	539:564	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	8	71	theme	corn	1453:1456	arg1	kernels					1458:1464	corn kernels	1453:1464	corn kernels	1453:1464	The ruminal in situ DM disappearance of unfermented kernels increased with the reduction in particle size of corn kernels.
26851843	16	72	theme	kernel	2600:2605	arg1	fraction					2607:2614	the kernel fraction	2596:2614	the kernel fraction	2596:2614	These findings suggest that hydrodynamic separation and dry sieving of the kernel fraction may provide a better assessment of kernel breakage in WPCS than CSPS.
26851843	2	73	from	degree	304:309	arg1	WPCS					333:336	WPCS	333:336	WPCS	333:336	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	15	74	theme	fraction	2479:2486	arg1	area					2505:2508	surface area	2497:2508	surface area	2497:2508	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed.
26851843	15	74	theme	fraction	2479:2486	arg1	proportion					2403:2412	proportion	2403:2412	proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS	2403:2491	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed.
26851843	9	75	theme	whole	1480:1484	arg1	Kernels					1467:1473	Kernels	1467:1473	Kernels kept whole	1467:1484	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	10	76	theme	varied	1721:1726	arg1	length					1740:1745	varied theoretical length	1721:1745	varied theoretical length of cut settings and processor types and settings	1721:1794	Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings were also evaluated.
26851843	6	77	dep	sieving	999:1005	arg1	determine					1010:1018	determine	1010:1018	to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities	1007:1239	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	12	78	theme	split	1970:1974	arg1	sample					1976:1981	the other split sample	1960:1981	the other split sample	1960:1981	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	6	79	theme	Dry	995:997	arg1	sieving					999:1005	Dry sieving	995:1005	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities	995:1239	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	2	80	theme	4.75-mm	264:270	arg1	sieve					272:276	a 4.75-mm sieve	262:276	a 4.75-mm sieve	262:276	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	9	81	theme	ruminal	1501:1507	arg1	disappearance					1512:1524	the lowest ruminal DM disappearance	1490:1524	the lowest ruminal DM disappearance for all time points	1490:1544	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	4	82	theme	kernels	587:593	arg1	digestibility					570:582	digestibility	570:582	digestibility	570:582	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	4	82	theme	kernels	587:593	arg1	distribution					553:564	particle size distribution	539:564	particle size distribution	539:564	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	6	83	theme	nominal	1091:1097	arg1	apertures					1106:1114	nominal square apertures	1091:1114	nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm	1091:1171	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	6	84	theme	as	1182:1183	arg1	GMPS					1020:1023	GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well	1020:1188	GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities	1020:1239	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	14	85	theme	fraction	2274:2281	arg1	GMPS					2283:2286	kernel fraction GMPS	2267:2286	kernel fraction GMPS	2267:2286	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor.
26851843	5	86	dep	kept	884:887	arg1	whole					889:893	whole	889:893	whole	889:893	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production were kept whole (WH) or manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively).
26851843	5	86	dep	kept	884:887	arg1	cut					912:914	cut	912:914	manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively)	903:992	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production were kept whole (WH) or manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively).
26851843	16	87	theme	better	2630:2635	arg1	assessment					2637:2646	a better assessment	2628:2646	a better assessment of kernel breakage in WPCS than CSPS	2628:2683	These findings suggest that hydrodynamic separation and dry sieving of the kernel fraction may provide a better assessment of kernel breakage in WPCS than CSPS.
26851843	11	88	theme	WPCS	1822:1825	arg1	sample					1827:1832	Each WPCS sample	1817:1832	Each WPCS sample	1817:1832	Each WPCS sample was divided in 2 and then dried at 60 °C for 48 h.
26851843	6	89	theme	particle	1044:1051	arg1	distribution					1058:1069	particle size distribution	1044:1069	particle size distribution	1044:1069	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	6	90	theme	mm	1170:1171	arg1	apertures					1106:1114	nominal square apertures	1091:1114	nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm	1091:1171	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	8	91	theme	kernels	1396:1402	arg1	disappearance					1367:1379	The ruminal in situ DM disappearance	1344:1379	The ruminal in situ DM disappearance of unfermented kernels	1344:1402	The ruminal in situ DM disappearance of unfermented kernels increased with the reduction in particle size of corn kernels.
26851843	5	92	theme	Composite	773:781	arg1	samples					783:789	Composite samples	773:789	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production	773:877	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production were kept whole (WH) or manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively).
26851843	4	93	theme	fraction	755:762	arg1	CSPS					727:730	CSPS	727:730	CSPS	727:730	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	4	93	theme	fraction	755:762	arg1	GMPS					736:739	GMPS	736:739	GMPS of the kernel fraction in WPCS	736:770	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	9	94	theme	maximum	1551:1557	arg1	disappearance					1562:1574	maximum DM disappearance	1551:1574	maximum DM disappearance of 6.9% at 24 h	1551:1590	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	6	95	theme	surface	1026:1032	arg1	area					1034:1037	surface area	1026:1037	surface area	1026:1037	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	8	96	theme	in	1356:1357	arg1	disappearance					1367:1379	The ruminal in situ DM disappearance	1344:1379	The ruminal in situ DM disappearance of unfermented kernels	1344:1402	The ruminal in situ DM disappearance of unfermented kernels increased with the reduction in particle size of corn kernels.
26851843	6	97	from	digestibilities	1225:1239	arg1	ruminal					1193:1199	ruminal	1193:1199	ruminal	1193:1199	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	15	98	theme	quadratic	2371:2379	arg1	relationships					2381:2393	Strong quadratic relationships	2364:2393	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area	2364:2508	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed.
26851843	0	99	theme	whole-plant	75:85	arg1	silage					92:97	whole-plant corn silage	75:97	whole-plant corn silage	75:97	Relationship between processing score and kernel-fraction particle size in whole-plant corn silage.
26851843	2	100	theme	starch	239:244	arg1	score					207:211	Corn silage processing score	184:211	Corn silage processing score (CSPS)	184:218	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	2	100	theme	starch	239:244	arg1	percentage					225:234	the percentage	221:234	the percentage of starch passing through a 4.75-mm sieve	221:276	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	6	101	theme	dry	1209:1211	arg1	digestibilities					1225:1239	situ dry matter (DM) digestibilities	1204:1239	situ dry matter (DM) digestibilities	1204:1239	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	1	102	theme	corn	164:167	arg1	silage					169:174	whole-plant corn silage	152:174	whole-plant corn silage (WPCS)	152:181	Kernel processing increases starch digestibility in whole-plant corn silage (WPCS).
26851843	1	102	theme	corn	164:167	arg1	WPCS					177:180	WPCS	177:180	WPCS	177:180	Kernel processing increases starch digestibility in whole-plant corn silage (WPCS).
26851843	16	103	theme	dry	2581:2583	arg1	sieving					2585:2591	dry sieving	2581:2591	dry sieving	2581:2591	These findings suggest that hydrodynamic separation and dry sieving of the kernel fraction may provide a better assessment of kernel breakage in WPCS than CSPS.
26851843	9	104	dep	observed	1627:1634	arg1	followed					1645:1652	followed	1645:1652	followed by 32P and 16P	1645:1667	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	10	105	theme	theoretical	1728:1738	arg1	length					1740:1745	varied theoretical length	1721:1745	varied theoretical length of cut settings and processor types and settings	1721:1794	Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings were also evaluated.
26851843	4	106	theme	particle	609:616	arg1	sizes					618:622	varied particle sizes	602:622	varied particle sizes	602:622	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	6	107	theme	square	1099:1104	arg1	apertures					1106:1114	nominal square apertures	1091:1114	nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm	1091:1171	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	6	108	with	sieves	1079:1084	arg1	apertures					1106:1114	nominal square apertures	1091:1114	nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm	1091:1171	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	10	109	theme	processor	1767:1775	arg1	types					1777:1781	processor types	1767:1781	processor types	1767:1781	Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings were also evaluated.
26851843	8	110	from	reduction	1423:1431	arg1	size					1445:1448	particle size	1436:1448	particle size of corn kernels	1436:1464	The ruminal in situ DM disappearance of unfermented kernels increased with the reduction in particle size of corn kernels.
26851843	13	111	theme	kernel	2095:2100	arg1	fraction					2102:2109	the kernel fraction	2091:2109	the kernel fraction	2091:2109	After separation, the kernel fraction was redried at 60°C for 48 h in a forced-air oven and dry sieved to determine GMPS and surface area.
26851843	4	112	from	GMPS	736:739	arg1	WPCS					767:770	WPCS	767:770	WPCS	767:770	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	6	113	theme	number	1281:1286	arg1	treatment					1288:1296	each kernel particle number treatment	1260:1296	each kernel particle number treatment	1260:1296	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	10	114	theme	settings	1754:1761	arg1	length					1740:1745	varied theoretical length	1721:1745	varied theoretical length of cut settings and processor types and settings	1721:1794	Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings were also evaluated.
26851843	5	115	dep	unfermented	794:804	arg1	dried					807:811	dried	807:811	dried	807:811	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production were kept whole (WH) or manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively).
26851843	4	116	from	CSPS	727:730	arg1	WPCS					767:770	WPCS	767:770	WPCS	767:770	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	4	117	from	GMPS	660:663	arg1	kernels					673:679	WPCS kernels	668:679	WPCS kernels	668:679	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	6	118	from	ruminal	1193:1199	arg1	digestibilities					1225:1239	situ dry matter (DM) digestibilities	1204:1239	situ dry matter (DM) digestibilities	1204:1239	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	14	119	theme	Linear	2212:2217	arg1	relationships					2219:2231	Linear relationships	2212:2231	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen	2212:2351	Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor.
26851843	12	120	theme	separation	2051:2060	arg1	procedure					2062:2070	a hydrodynamic separation procedure	2036:2070	a hydrodynamic separation procedure	2036:2070	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	4	121	theme	WPCS	668:671	arg1	kernels					673:679	WPCS kernels	668:679	WPCS kernels	668:679	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	16	122	theme	kernel	2651:2656	arg1	breakage					2658:2665	kernel breakage	2651:2665	kernel breakage	2651:2665	These findings suggest that hydrodynamic separation and dry sieving of the kernel fraction may provide a better assessment of kernel breakage in WPCS than CSPS.
26851843	4	123	dep	propose	632:638	arg1	2					626:626	2	626:626	2	626:626	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	9	124	from	disappearance	1562:1574	arg1	h					1590:1590	24 h	1587:1590	24 h	1587:1590	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	3	125	theme	geometric	352:360	arg1	GMPS					382:385	GMPS	382:385	GMPS	382:385	However, the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve has not been well described.
26851843	3	125	theme	geometric	352:360	arg1	size					376:379	the geometric mean particle size	348:379	the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve	348:447	However, the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve has not been well described.
26851843	6	126	theme	kernel	1265:1270	arg1	treatment					1288:1296	each kernel particle number treatment	1260:1296	each kernel particle number treatment	1260:1296	Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment.
26851843	5	127	theme	kernels	813:819	arg1	samples					783:789	Composite samples	773:789	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production	773:877	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production were kept whole (WH) or manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively).
26851843	4	128	theme	study	512:516	arg1	objectives					493:502	the objectives	489:502	the objectives of this study	489:516	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	12	129	theme	hydrodynamic	2038:2049	arg1	procedure					2062:2070	a hydrodynamic separation procedure	2036:2070	a hydrodynamic separation procedure	2036:2070	The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure.
26851843	2	130	theme	silage	189:194	arg1	score					207:211	Corn silage processing score	184:211	Corn silage processing score (CSPS)	184:218	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	2	130	theme	silage	189:194	arg1	percentage					225:234	the percentage	221:234	the percentage of starch passing through a 4.75-mm sieve	221:276	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	2	130	theme	silage	189:194	arg1	CSPS					214:217	CSPS	214:217	CSPS	214:217	Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS.
26851843	10	131	theme	WPCS	1681:1684	arg1	Samples					1670:1676	Samples	1670:1676	Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings	1670:1794	Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings were also evaluated.
26851843	0	132	theme	kernel-fraction	42:56	arg1	size					67:70	kernel-fraction particle size	42:70	kernel-fraction particle size	42:70	Relationship between processing score and kernel-fraction particle size in whole-plant corn silage.
26851843	7	133	theme	Incubation	1299:1308	arg1	times					1310:1314	Incubation times	1299:1314	Incubation times	1299:1314	Incubation times were 0, 3, 6, 12, and 24 h.
26851843	15	134	theme	kernel	2417:2422	arg1	fraction					2424:2431	kernel fraction	2417:2431	kernel fraction passing through the 4.75-mm screen	2417:2466	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed.
26851843	3	135	theme	particle	367:374	arg1	GMPS					382:385	GMPS	382:385	GMPS	382:385	However, the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve has not been well described.
26851843	3	135	theme	particle	367:374	arg1	size					376:379	the geometric mean particle size	348:379	the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve	348:447	However, the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve has not been well described.
26851843	9	136	contain	had	1486:1488	arg2	disappearance					1512:1524	the lowest ruminal DM disappearance	1490:1524	the lowest ruminal DM disappearance for all time points	1490:1544	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	9	136	contain	had	1486:1488	arg1	Kernels					1467:1473	Kernels	1467:1473	Kernels kept whole	1467:1484	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	13	137	theme	surface	2198:2204	arg1	area					2206:2209	surface area	2198:2209	surface area	2198:2209	After separation, the kernel fraction was redried at 60°C for 48 h in a forced-air oven and dry sieved to determine GMPS and surface area.
26851843	15	138	theme	surface	2497:2503	arg1	area					2505:2508	surface area	2497:2508	surface area	2497:2508	Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed.
26851843	3	139	theme	4.75-mm	435:441	arg1	sieve					443:447	the 4.75-mm sieve	431:447	the 4.75-mm sieve	431:447	However, the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve has not been well described.
26851843	5	140	theme	silage	861:866	arg1	production					868:877	silage production	861:877	silage production	861:877	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production were kept whole (WH) or manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively).
26851843	16	141	theme	fraction	2607:2614	arg1	separation					2566:2575	hydrodynamic separation	2553:2575	hydrodynamic separation	2553:2575	These findings suggest that hydrodynamic separation and dry sieving of the kernel fraction may provide a better assessment of kernel breakage in WPCS than CSPS.
26851843	16	141	theme	fraction	2607:2614	arg1	sieving					2585:2591	dry sieving	2581:2591	dry sieving	2581:2591	These findings suggest that hydrodynamic separation and dry sieving of the kernel fraction may provide a better assessment of kernel breakage in WPCS than CSPS.
26851843	9	142	from	h	1590:1590	arg1	disappearance					1562:1574	maximum DM disappearance	1551:1574	maximum DM disappearance of 6.9% at 24 h	1551:1590	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	9	142	from	h	1590:1590	arg1	%					1582:1582	6.9%	1579:1582	6.9% at 24 h	1579:1590	Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P.
26851843	4	143	theme	size	548:551	arg1	distribution					553:564	particle size distribution	539:564	particle size distribution	539:564	Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS.
26851843	5	144	from	hybrids	835:841	arg1	samples					783:789	Composite samples	773:789	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production	773:877	Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production were kept whole (WH) or manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively).
27599464	7	0	theme	data	1154:1157	arg1	group					1145:1149	each group	1140:1149	each group of data from CHO sample and human testing sample	1140:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	7	0	theme	data	1154:1157	arg1	data					1154:1157	data	1154:1157	data from CHO sample and human testing sample	1154:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	6	1	theme	detected	924:931	arg1	ions					933:936	two detected ions	920:936	two detected ions	920:936	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	3	2	dep	METHODS	347:353	arg1	report					365:370	report	365:370	report a tool for automatically annotating and browsing N-glycan masses and isotopic distributions	365:462	METHODS Herein we report a tool for automatically annotating and browsing N-glycan masses and isotopic distributions.
27599464	5	3	dep	region	748:753	arg1	overlap					740:746	overlap	740:746	overlap	740:746	In addition, we improved a matching glycan isotope abundance algorithm through identifying potential overlap region and constructing an optimization model so that it can deconvolute the overlapped glycan isotopic clusters.
27599464	1	4	theme	glycan	146:151	arg1	structures					153:162	glycan structures	146:162	glycan structures	146:162	RATIONALE With the development of glycomics, a large number of glycan structures have been determined by using mass spectrometry (MS)-based techniques.
27599464	9	5	from	increase	1499:1506	arg1	sensitivity					1522:1532	annotation sensitivity	1511:1532	annotation sensitivity	1511:1532	The results demonstrated an average of 26.8% increase in annotation sensitivity through the SMOTE-SVMs algorithm.
27599464	6	6	theme	m/z	979:981	arg1	RESULTS					862:868	RESULTS	862:868	RESULTS In the matching process	862:892	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	6	theme	m/z	979:981	arg1	region					1030:1035	a potential overlapped region	1007:1035	a potential overlapped region	1007:1035	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	6	theme	m/z	979:981	arg1	range					983:987	the m/z range	975:987	the m/z range	975:987	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	7	dep	5	972:972	arg1	to					969:970	to	969:970	to	969:970	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	8	theme	ions	933:936	arg1	difference					906:915	the m/z difference	898:915	the m/z difference of two detected ions	898:936	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	8	theme	ions	933:936	arg1	integer					954:960	an integer	951:960	an integer from 1 to 5	951:972	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	7	9	dep	potential	1111:1119	arg1	overlap					1121:1127	overlap	1121:1127	overlap regions in each group of data from CHO sample and human testing sample	1121:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	1	10	theme	structures	153:162	arg1	glycomics					117:125	glycomics	117:125	glycomics	117:125	RATIONALE With the development of glycomics, a large number of glycan structures have been determined by using mass spectrometry (MS)-based techniques.
27599464	1	10	theme	structures	153:162	arg1	number					136:141	a large number	128:141	a large number of glycan structures	128:162	RATIONALE With the development of glycomics, a large number of glycan structures have been determined by using mass spectrometry (MS)-based techniques.
27599464	9	11	from	average	1482:1488	arg1	sensitivity					1522:1532	annotation sensitivity	1511:1532	annotation sensitivity	1511:1532	The results demonstrated an average of 26.8% increase in annotation sensitivity through the SMOTE-SVMs algorithm.
27599464	8	12	theme	Machines	1280:1287	arg1	algorithm					1296:1304	the Supporting Vector Machines (SVMs) algorithm	1258:1304	the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE),	1258:1402	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	4	13	theme	preprocessing	586:598	arg1	data					581:584	data	581:584	data preprocessing and filtering by composition matching	581:636	We first constructed a training dataset using the Consortium for Functional Glycomics database, in conjunction with data preprocessing and filtering by composition matching.
27599464	8	14	theme	Vector	1273:1278	arg1	SVMs					1290:1293	SVMs	1290:1293	SVMs	1290:1293	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	8	14	theme	Vector	1273:1278	arg1	Machines					1280:1287	Supporting Vector Machines	1262:1287	the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE),	1258:1402	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	8	15	theme	Supporting	1262:1271	arg1	SVMs					1290:1293	SVMs	1290:1293	SVMs	1290:1293	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	8	15	theme	Supporting	1262:1271	arg1	Machines					1280:1287	Supporting Vector Machines	1262:1287	the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE),	1258:1402	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	11	16	theme	mass	1755:1758	arg1	spectrometrists					1760:1774	mass spectrometrists	1755:1774	mass spectrometrists	1755:1774	CONCLUSIONS We have developed a new tool which facilities high-throughput glycomics research and assists mass spectrometrists in the interpretation and annotation of glycan samples.
27599464	12	17	dep	&	1860:1860	arg1	Sons					1862:1865	Sons	1862:1865	Sons	1862:1865	Copyright © 2016 John Wiley & Sons, Ltd.
27599464	6	18	theme	m/z	902:904	arg1	difference					906:915	the m/z difference	898:915	the m/z difference of two detected ions	898:936	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	18	theme	m/z	902:904	arg1	integer					954:960	an integer	951:960	an integer from 1 to 5	951:972	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	19	theme	overlapped	1019:1028	arg1	RESULTS					862:868	RESULTS	862:868	RESULTS In the matching process	862:892	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	19	theme	overlapped	1019:1028	arg1	region					1030:1035	a potential overlapped region	1007:1035	a potential overlapped region	1007:1035	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	19	theme	overlapped	1019:1028	arg1	range					983:987	the m/z range	975:987	the m/z range	975:987	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	2	20	theme	MS	256:257	arg1	data					259:262	most glycan MS data	244:262	most glycan MS data	244:262	However, most glycan MS data needs to be manually annotated which is time-consuming, unreliable and inaccurate.
27599464	6	21	theme	lower	1047:1051	arg1	m/z					1053:1055	the lower m/z	1043:1055	the lower m/z to m/z + 5	1043:1066	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	22	from	RESULTS	862:868	arg1	process					886:892	the matching process	873:892	the matching process	873:892	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	23	from	5	972:972	arg1	difference					906:915	the m/z difference	898:915	the m/z difference of two detected ions	898:936	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	23	from	5	972:972	arg1	integer					954:960	an integer	951:960	an integer from 1 to 5	951:972	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	2	24	theme	glycan	249:254	arg1	data					259:262	most glycan MS data	244:262	most glycan MS data	244:262	However, most glycan MS data needs to be manually annotated which is time-consuming, unreliable and inaccurate.
27599464	3	25	theme	N-glycan	421:428	arg1	masses					430:435	N-glycan masses	421:435	N-glycan masses	421:435	METHODS Herein we report a tool for automatically annotating and browsing N-glycan masses and isotopic distributions.
27599464	7	26	from	regions	1129:1135	arg1	group					1145:1149	each group	1140:1149	each group of data from CHO sample and human testing sample	1140:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	7	26	from	regions	1129:1135	arg1	data					1154:1157	data	1154:1157	data from CHO sample and human testing sample	1154:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	9	27	theme	increase	1499:1506	arg1	average					1482:1488	an average	1479:1488	an average of 26.8% increase in annotation sensitivity	1479:1532	The results demonstrated an average of 26.8% increase in annotation sensitivity through the SMOTE-SVMs algorithm.
27599464	0	28	theme	Automatic	0:8	arg1	annotation					10:19	Automatic annotation and visualization tool	0:42	annotation	10:19	Automatic annotation and visualization tool for mass spectrometry based glycomics.
27599464	5	29	theme	overlapped	825:834	arg1	clusters					852:859	the overlapped glycan isotopic clusters	821:859	the overlapped glycan isotopic clusters	821:859	In addition, we improved a matching glycan isotope abundance algorithm through identifying potential overlap region and constructing an optimization model so that it can deconvolute the overlapped glycan isotopic clusters.
27599464	0	30	theme	visualization	25:37	arg1	tool					39:42	Automatic annotation and visualization tool	0:42	tool	39:42	Automatic annotation and visualization tool for mass spectrometry based glycomics.
27599464	4	31	theme	Glycomics	541:549	arg1	database					551:558	Functional Glycomics database	530:558	Functional Glycomics database	530:558	We first constructed a training dataset using the Consortium for Functional Glycomics database, in conjunction with data preprocessing and filtering by composition matching.
27599464	11	32	theme	new	1682:1684	arg1	tool					1686:1689	a new tool	1680:1689	a new tool which facilities high-throughput glycomics research and assists mass spectrometrists in the interpretation and annotation of glycan samples	1680:1829	CONCLUSIONS We have developed a new tool which facilities high-throughput glycomics research and assists mass spectrometrists in the interpretation and annotation of glycan samples.
27599464	7	33	from	sample	1193:1198	arg1	group					1145:1149	each group	1140:1149	each group of data from CHO sample and human testing sample	1140:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	7	33	from	sample	1193:1198	arg1	data					1154:1157	data	1154:1157	data from CHO sample and human testing sample	1154:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	8	34	theme	different	1311:1319	arg1	techniques					1330:1339	different sampling techniques	1311:1339	different sampling techniques	1311:1339	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	8	34	theme	different	1311:1319	arg1	Technique					1385:1393	Synthetic Minority Over-sampling Technique	1352:1393	Synthetic Minority Over-sampling Technique (SMOTE)	1352:1401	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	3	35	theme	isotopic	441:448	arg1	distributions					450:462	isotopic distributions	441:462	isotopic distributions	441:462	METHODS Herein we report a tool for automatically annotating and browsing N-glycan masses and isotopic distributions.
27599464	5	36	theme	isotopic	843:850	arg1	clusters					852:859	the overlapped glycan isotopic clusters	821:859	the overlapped glycan isotopic clusters	821:859	In addition, we improved a matching glycan isotope abundance algorithm through identifying potential overlap region and constructing an optimization model so that it can deconvolute the overlapped glycan isotopic clusters.
27599464	9	37	theme	%	1497:1497	arg1	increase					1499:1506	26.8% increase	1493:1506	26.8% increase in annotation sensitivity	1493:1532	The results demonstrated an average of 26.8% increase in annotation sensitivity through the SMOTE-SVMs algorithm.
27599464	11	38	dep	CONCLUSIONS	1650:1660	arg1	developed					1670:1678	developed	1670:1678	have developed a new tool which facilities high-throughput glycomics research and assists mass spectrometrists in the interpretation and annotation of glycan samples	1665:1829	CONCLUSIONS We have developed a new tool which facilities high-throughput glycomics research and assists mass spectrometrists in the interpretation and annotation of glycan samples.
27599464	7	39	theme	human	1179:1183	arg1	sample					1193:1198	human testing sample	1179:1198	human testing sample	1179:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	1	40	theme	mass	194:197	arg1	MS					213:214	MS	213:214	MS	213:214	RATIONALE With the development of glycomics, a large number of glycan structures have been determined by using mass spectrometry (MS)-based techniques.
27599464	1	40	theme	mass	194:197	arg1	spectrometry					199:210	mass spectrometry	194:210	mass spectrometry (MS)	194:215	RATIONALE With the development of glycomics, a large number of glycan structures have been determined by using mass spectrometry (MS)-based techniques.
27599464	8	41	theme	training	1213:1220	arg1	imbalanced					1234:1243	imbalanced	1234:1243	imbalanced	1234:1243	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	8	41	theme	training	1213:1220	arg1	dataset					1222:1228	the training dataset	1209:1228	the training dataset	1209:1228	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	9	42	theme	SMOTE-SVMs	1546:1555	arg1	algorithm					1557:1565	the SMOTE-SVMs algorithm	1542:1565	the SMOTE-SVMs algorithm	1542:1565	The results demonstrated an average of 26.8% increase in annotation sensitivity through the SMOTE-SVMs algorithm.
27599464	6	43	theme	potential	1009:1017	arg1	RESULTS					862:868	RESULTS	862:868	RESULTS In the matching process	862:892	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	43	theme	potential	1009:1017	arg1	region					1030:1035	a potential overlapped region	1007:1035	a potential overlapped region	1007:1035	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	43	theme	potential	1009:1017	arg1	range					983:987	the m/z range	975:987	the m/z range	975:987	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	1	44	with	RATIONALE	83:91	arg1	development					102:112	the development	98:112	the development of glycomics, a large number of glycan structures	98:162	RATIONALE With the development of glycomics, a large number of glycan structures have been determined by using mass spectrometry (MS)-based techniques.
27599464	11	45	theme	samples	1823:1829	arg1	annotation					1802:1811	annotation	1802:1811	annotation	1802:1811	CONCLUSIONS We have developed a new tool which facilities high-throughput glycomics research and assists mass spectrometrists in the interpretation and annotation of glycan samples.
27599464	11	45	theme	samples	1823:1829	arg1	interpretation					1783:1796	interpretation	1783:1796	interpretation	1783:1796	CONCLUSIONS We have developed a new tool which facilities high-throughput glycomics research and assists mass spectrometrists in the interpretation and annotation of glycan samples.
27599464	5	46	theme	potential	730:738	arg1	region					748:753	potential overlap region	730:753	potential overlap region	730:753	In addition, we improved a matching glycan isotope abundance algorithm through identifying potential overlap region and constructing an optimization model so that it can deconvolute the overlapped glycan isotopic clusters.
27599464	6	47	theme	matching	877:884	arg1	process					886:892	the matching process	873:892	the matching process	873:892	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	5	48	theme	glycan	836:841	arg1	clusters					852:859	the overlapped glycan isotopic clusters	821:859	the overlapped glycan isotopic clusters	821:859	In addition, we improved a matching glycan isotope abundance algorithm through identifying potential overlap region and constructing an optimization model so that it can deconvolute the overlapped glycan isotopic clusters.
27599464	8	49	with	algorithm	1296:1304	arg1	techniques					1330:1339	different sampling techniques	1311:1339	different sampling techniques	1311:1339	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	8	49	with	algorithm	1296:1304	arg1	Technique					1385:1393	Synthetic Minority Over-sampling Technique	1352:1393	Synthetic Minority Over-sampling Technique (SMOTE)	1352:1401	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	11	50	theme	glycan	1816:1821	arg1	samples					1823:1829	glycan samples	1816:1829	glycan samples	1816:1829	CONCLUSIONS We have developed a new tool which facilities high-throughput glycomics research and assists mass spectrometrists in the interpretation and annotation of glycan samples.
27599464	5	51	theme	matching	666:673	arg1	algorithm					700:708	a matching glycan isotope abundance algorithm	664:708	a matching glycan isotope abundance algorithm	664:708	In addition, we improved a matching glycan isotope abundance algorithm through identifying potential overlap region and constructing an optimization model so that it can deconvolute the overlapped glycan isotopic clusters.
27599464	8	52	theme	candidate	1430:1438	arg1	compositions					1440:1451	all potential candidate compositions	1416:1451	all potential candidate compositions	1416:1451	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	11	53	theme	high-throughput	1708:1722	arg1	research					1734:1741	high-throughput glycomics research	1708:1741	high-throughput glycomics research	1708:1741	CONCLUSIONS We have developed a new tool which facilities high-throughput glycomics research and assists mass spectrometrists in the interpretation and annotation of glycan samples.
27599464	1	54	theme	glycomics	117:125	arg1	development					102:112	the development	98:112	the development of glycomics, a large number of glycan structures	98:162	RATIONALE With the development of glycomics, a large number of glycan structures have been determined by using mass spectrometry (MS)-based techniques.
27599464	0	55	theme	mass	48:51	arg1	spectrometry					53:64	mass spectrometry	48:64	mass spectrometry	48:64	Automatic annotation and visualization tool for mass spectrometry based glycomics.
27599464	4	56	theme	Functional	530:539	arg1	database					551:558	Functional Glycomics database	530:558	Functional Glycomics database	530:558	We first constructed a training dataset using the Consortium for Functional Glycomics database, in conjunction with data preprocessing and filtering by composition matching.
27599464	5	57	theme	glycan	675:680	arg1	algorithm					700:708	a matching glycan isotope abundance algorithm	664:708	a matching glycan isotope abundance algorithm	664:708	In addition, we improved a matching glycan isotope abundance algorithm through identifying potential overlap region and constructing an optimization model so that it can deconvolute the overlapped glycan isotopic clusters.
27599464	11	58	dep	interpretation	1783:1796	arg1	the					1779:1781	the	1779:1781	the	1779:1781	CONCLUSIONS We have developed a new tool which facilities high-throughput glycomics research and assists mass spectrometrists in the interpretation and annotation of glycan samples.
27599464	7	59	from	group	1145:1149	arg1	sample					1193:1198	human testing sample	1179:1198	human testing sample	1179:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	7	59	from	group	1145:1149	arg1	sample					1168:1173	CHO sample	1164:1173	CHO sample	1164:1173	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	9	60	from	sensitivity	1522:1532	arg1	average					1482:1488	an average	1479:1488	an average of 26.8% increase in annotation sensitivity	1479:1532	The results demonstrated an average of 26.8% increase in annotation sensitivity through the SMOTE-SVMs algorithm.
27599464	8	61	theme	Over-sampling	1371:1383	arg1	SMOTE					1396:1400	SMOTE	1396:1400	SMOTE	1396:1400	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	8	61	theme	Over-sampling	1371:1383	arg1	Technique					1385:1393	Synthetic Minority Over-sampling Technique	1352:1393	Synthetic Minority Over-sampling Technique (SMOTE)	1352:1401	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	5	62	theme	isotope	682:688	arg1	algorithm					700:708	a matching glycan isotope abundance algorithm	664:708	a matching glycan isotope abundance algorithm	664:708	In addition, we improved a matching glycan isotope abundance algorithm through identifying potential overlap region and constructing an optimization model so that it can deconvolute the overlapped glycan isotopic clusters.
27599464	8	63	theme	Minority	1362:1369	arg1	SMOTE					1396:1400	SMOTE	1396:1400	SMOTE	1396:1400	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	8	63	theme	Minority	1362:1369	arg1	Technique					1385:1393	Synthetic Minority Over-sampling Technique	1352:1393	Synthetic Minority Over-sampling Technique (SMOTE)	1352:1401	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	2	64	theme	most	244:247	arg1	data					259:262	most glycan MS data	244:262	most glycan MS data	244:262	However, most glycan MS data needs to be manually annotated which is time-consuming, unreliable and inaccurate.
27599464	8	65	theme	sampling	1321:1328	arg1	techniques					1330:1339	different sampling techniques	1311:1339	different sampling techniques	1311:1339	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	8	65	theme	sampling	1321:1328	arg1	Technique					1385:1393	Synthetic Minority Over-sampling Technique	1352:1393	Synthetic Minority Over-sampling Technique (SMOTE)	1352:1401	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	9	66	theme	annotation	1511:1520	arg1	sensitivity					1522:1532	annotation sensitivity	1511:1532	annotation sensitivity	1511:1532	The results demonstrated an average of 26.8% increase in annotation sensitivity through the SMOTE-SVMs algorithm.
27599464	4	67	theme	filtering	604:612	arg1	data					581:584	data	581:584	data preprocessing and filtering by composition matching	581:636	We first constructed a training dataset using the Consortium for Functional Glycomics database, in conjunction with data preprocessing and filtering by composition matching.
27599464	5	68	theme	abundance	690:698	arg1	algorithm					700:708	a matching glycan isotope abundance algorithm	664:708	a matching glycan isotope abundance algorithm	664:708	In addition, we improved a matching glycan isotope abundance algorithm through identifying potential overlap region and constructing an optimization model so that it can deconvolute the overlapped glycan isotopic clusters.
27599464	8	69	theme	potential	1420:1428	arg1	compositions					1440:1451	all potential candidate compositions	1416:1451	all potential candidate compositions	1416:1451	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	8	70	theme	Synthetic	1352:1360	arg1	SMOTE					1396:1400	SMOTE	1396:1400	SMOTE	1396:1400	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	8	70	theme	Synthetic	1352:1360	arg1	Technique					1385:1393	Synthetic Minority Over-sampling Technique	1352:1393	Synthetic Minority Over-sampling Technique (SMOTE)	1352:1401	Because the training dataset was imbalanced, we combined the Supporting Vector Machines (SVMs) algorithm with different sampling techniques, including Synthetic Minority Over-sampling Technique (SMOTE), to classify all potential candidate compositions.
27599464	1	71	theme	large	130:134	arg1	glycomics					117:125	glycomics	117:125	glycomics	117:125	RATIONALE With the development of glycomics, a large number of glycan structures have been determined by using mass spectrometry (MS)-based techniques.
27599464	1	71	theme	large	130:134	arg1	number					136:141	a large number	128:141	a large number of glycan structures	128:162	RATIONALE With the development of glycomics, a large number of glycan structures have been determined by using mass spectrometry (MS)-based techniques.
27599464	7	72	theme	CHO	1164:1166	arg1	sample					1168:1173	CHO sample	1164:1173	CHO sample	1164:1173	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	7	73	from	sample	1168:1173	arg1	group					1145:1149	each group	1140:1149	each group of data from CHO sample and human testing sample	1140:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	7	73	from	sample	1168:1173	arg1	data					1154:1157	data	1154:1157	data from CHO sample and human testing sample	1154:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	10	74	dep	https	1605:1609	arg1	//sourceforge.net/projects/glycomaid/					1611:1647	//sourceforge.net/projects/glycomaid/	1611:1647	https://sourceforge.net/projects/glycomaid/	1605:1647	The source code can be obtained from https://sourceforge.net/projects/glycomaid/.
27599464	6	75	from	m/z	1053:1055	arg1	RESULTS					862:868	RESULTS	862:868	RESULTS In the matching process	862:892	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	75	from	m/z	1053:1055	arg1	region					1030:1035	a potential overlapped region	1007:1035	a potential overlapped region	1007:1035	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	6	75	from	m/z	1053:1055	arg1	range					983:987	the m/z range	975:987	the m/z range	975:987	RESULTS In the matching process, if the m/z difference of two detected ions was close to an integer from 1 to 5, the m/z range was considered as a potential overlapped region, from the lower m/z to m/z + 5.
27599464	7	76	theme	testing	1185:1191	arg1	sample					1193:1198	human testing sample	1179:1198	human testing sample	1179:1198	It was found that there were more than 20 potential overlap regions in each group of data from CHO sample and human testing sample.
27599464	1	77	theme	-based	216:221	arg1	techniques					223:232	mass spectrometry (MS)-based techniques	194:232	mass spectrometry (MS)-based techniques	194:232	RATIONALE With the development of glycomics, a large number of glycan structures have been determined by using mass spectrometry (MS)-based techniques.
27599464	5	78	theme	optimization	775:786	arg1	model					788:792	an optimization model	772:792	an optimization model so that it can deconvolute the overlapped glycan isotopic clusters	772:859	In addition, we improved a matching glycan isotope abundance algorithm through identifying potential overlap region and constructing an optimization model so that it can deconvolute the overlapped glycan isotopic clusters.
27599464	4	79	theme	training	488:495	arg1	dataset					497:503	a training dataset	486:503	a training dataset	486:503	We first constructed a training dataset using the Consortium for Functional Glycomics database, in conjunction with data preprocessing and filtering by composition matching.
27599464	4	80	with	conjunction	564:574	arg1	data					581:584	data	581:584	data preprocessing and filtering by composition matching	581:636	We first constructed a training dataset using the Consortium for Functional Glycomics database, in conjunction with data preprocessing and filtering by composition matching.
27599464	9	81	theme	26.8	1493:1496	arg1	%					1497:1497	%	1497:1497	%	1497:1497	The results demonstrated an average of 26.8% increase in annotation sensitivity through the SMOTE-SVMs algorithm.
27599464	11	82	theme	glycomics	1724:1732	arg1	research					1734:1741	high-throughput glycomics research	1708:1741	high-throughput glycomics research	1708:1741	CONCLUSIONS We have developed a new tool which facilities high-throughput glycomics research and assists mass spectrometrists in the interpretation and annotation of glycan samples.
29131593	8	0	theme	friendly	1552:1559	arg1	systems					1569:1575	environmentally friendly complex systems	1536:1575	environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems	1536:1690	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	6	1	theme	CNF	1193:1195	arg1	CNF					1193:1195	CNF	1193:1195	CNF	1193:1195	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	6	1	theme	CNF	1193:1195	arg1	amounts					1182:1188	small amounts	1176:1188	small amounts of CNF	1176:1195	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	8	2	theme	ionic	1620:1624	arg1	strength					1626:1633	ionic strength	1620:1633	ionic strength	1620:1633	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	6	3	theme	amounts	1182:1188	arg1	possible					1108:1115	possible	1108:1115	possible	1108:1115	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	7	4	theme	fiber	1325:1329	arg1	deconstruction					1331:1344	fiber deconstruction	1325:1344	fiber deconstruction	1325:1344	The results revealed some subtle differences in CNF interfacial activity, depending on the method used for their isolation via fiber deconstruction, either from microfluidization or aqueous counter collision.
29131593	8	5	theme	classical	1665:1673	arg1	systems					1684:1690	classical emulsion systems	1665:1690	classical emulsion systems	1665:1690	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	8	6	theme	systems	1684:1690	arg1	expectations					1649:1660	the expectations	1645:1660	the expectations of classical emulsion systems	1645:1690	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	3	7	theme	phase	682:686	arg1	behavior					688:695	the phase behavior	678:695	the phase behavior	678:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	1	8	from	application	255:265	arg1	pharma					276:281	pharma	276:281	pharma	276:281	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	1	8	from	application	255:265	arg1	cosmetics					288:296	cosmetics	288:296	cosmetics	288:296	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	1	8	from	application	255:265	arg1	food					270:273	food	270:273	food	270:273	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	1	9	theme	colloidal	210:218	arg1	dispersions					220:230	colloidal dispersions	210:230	colloidal dispersions	210:230	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	7	10	dep	collision	1396:1404	arg1	counter					1388:1394	counter	1388:1394	counter	1388:1394	The results revealed some subtle differences in CNF interfacial activity, depending on the method used for their isolation via fiber deconstruction, either from microfluidization or aqueous counter collision.
29131593	6	11	theme	SDS	1138:1140	arg1	concentrations					1142:1155	SDS concentrations	1138:1155	SDS concentrations	1138:1155	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	2	12	used	used	368:371	arg2	emulsifier					376:385	emulsifier	376:385	emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF	376:478	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	2	12	used	used	368:371	arg2	surfactant					323:332	an ionic surfactant	314:332	an ionic surfactant (sodium dodecyl sulfate, SDS)	314:362	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	4	13	theme	emulsions	805:813	arg1	transition					791:800	the phase transition	781:800	the phase transition of emulsions containing CNF and SDS at low concentrations	781:858	At low salinity, the phase transition of emulsions containing CNF and SDS at low concentrations was controlled by molecular transfer in the oil-in-water system.
29131593	5	14	theme	"	962:962	arg1	morphologies					964:975	"bi-continuous" morphologies	948:975	"bi-continuous" morphologies	948:975	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	3	15	theme	Windsor	747:753	arg1	systems					755:761	Windsor systems	747:761	Windsor systems	747:761	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	7	16	theme	interfacial	1250:1260	arg1	activity					1262:1269	CNF interfacial activity	1246:1269	CNF interfacial activity	1246:1269	The results revealed some subtle differences in CNF interfacial activity, depending on the method used for their isolation via fiber deconstruction, either from microfluidization or aqueous counter collision.
29131593	6	17	from	concentrations	1142:1155	arg1	possible					1108:1115	possible	1108:1115	possible	1108:1115	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	0	18	theme	Emulsions	60:68	arg1	Effects					28:34	Formulation and Composition Effects	0:34	Formulation and Composition Effects in Phase Transitions of Emulsions	0:68	Formulation and Composition Effects in Phase Transitions of Emulsions Costabilized by Cellulose Nanofibrils and an Ionic Surfactant.
29131593	5	19	contain	containing	1031:1040	arg1	systems					1023:1029	systems	1023:1029	systems containing high CNF and SDS concentrations	1023:1072	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	5	19	contain	containing	1031:1040	arg2	concentrations					1059:1072	high CNF and SDS concentrations	1042:1072	high CNF and SDS concentrations	1042:1072	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	5	20	theme	high	1042:1045	arg1	concentrations					1059:1072	high CNF and SDS concentrations	1042:1072	high CNF and SDS concentrations	1042:1072	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	4	21	theme	low	841:843	arg1	concentrations					845:858	low concentrations	841:858	low concentrations	841:858	At low salinity, the phase transition of emulsions containing CNF and SDS at low concentrations was controlled by molecular transfer in the oil-in-water system.
29131593	7	22	theme	CNF	1246:1248	arg1	activity					1262:1269	CNF interfacial activity	1246:1269	CNF interfacial activity	1246:1269	The results revealed some subtle differences in CNF interfacial activity, depending on the method used for their isolation via fiber deconstruction, either from microfluidization or aqueous counter collision.
29131593	2	23	theme	water-in-oil	407:418	arg1	emulsions					420:428	oil-in-water and water-in-oil emulsions	390:428	oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF	390:478	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	3	24	from	influence	569:577	arg1	properties					496:505	The adsorption properties	481:505	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior	481:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	24	from	influence	569:577	arg1	behavior					688:695	the phase behavior	678:695	the phase behavior	678:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	0	25	theme	Formulation	0:10	arg1	Effects					28:34	Formulation and Composition Effects	0:34	Formulation and Composition Effects in Phase Transitions of Emulsions	0:68	Formulation and Composition Effects in Phase Transitions of Emulsions Costabilized by Cellulose Nanofibrils and an Ionic Surfactant.
29131593	2	26	theme	oil-in-water	390:401	arg1	emulsions					420:428	oil-in-water and water-in-oil emulsions	390:428	oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF	390:478	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	8	27	theme	morphology	1456:1465	arg1	control					1436:1442	the control	1432:1442	the control of emulsion morphology and stability by addition of CNF	1432:1498	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	3	28	from	emulsions	543:551	arg1	properties					496:505	The adsorption properties	481:505	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior	481:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	28	from	emulsions	543:551	arg1	behavior					688:695	the phase behavior	678:695	the phase behavior	678:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	0	29	theme	Composition	16:26	arg1	Effects					28:34	Formulation and Composition Effects	0:34	Formulation and Composition Effects in Phase Transitions of Emulsions	0:68	Formulation and Composition Effects in Phase Transitions of Emulsions Costabilized by Cellulose Nanofibrils and an Ionic Surfactant.
29131593	4	30	contain	containing	815:824	arg2	SDS					834:836	SDS	834:836	SDS	834:836	At low salinity, the phase transition of emulsions containing CNF and SDS at low concentrations was controlled by molecular transfer in the oil-in-water system.
29131593	4	30	contain	containing	815:824	arg2	CNF					826:828	CNF	826:828	CNF	826:828	At low salinity, the phase transition of emulsions containing CNF and SDS at low concentrations was controlled by molecular transfer in the oil-in-water system.
29131593	4	30	contain	containing	815:824	arg1	emulsions					805:813	emulsions	805:813	emulsions containing CNF and SDS at low concentrations	805:858	At low salinity, the phase transition of emulsions containing CNF and SDS at low concentrations was controlled by molecular transfer in the oil-in-water system.
29131593	8	31	theme	stability	1471:1479	arg1	control					1436:1442	the control	1432:1442	the control of emulsion morphology and stability by addition of CNF	1432:1498	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	3	32	from	dispersions	527:537	arg1	properties					496:505	The adsorption properties	481:505	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior	481:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	32	from	dispersions	527:537	arg1	behavior					688:695	the phase behavior	678:695	the phase behavior	678:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	5	33	located	observed	982:989	arg1	salinity					1010:1017	high salinity	1005:1017	high salinity	1005:1017	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	5	33	located	observed	982:989	arg2	droplets					935:942	Irregular droplets	925:942	Irregular droplets	925:942	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	5	33	located	observed	982:989	arg2	morphologies					964:975	"bi-continuous" morphologies	948:975	"bi-continuous" morphologies	948:975	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	5	33	located	observed	982:989	arg1	medium					994:999	medium	994:999	medium	994:999	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	5	34	theme	Irregular	925:933	arg1	droplets					935:942	Irregular droplets	925:942	Irregular droplets	925:942	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	3	35	theme	composition	582:592	arg1	influence					569:577	the influence	565:577	the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior	565:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	35	theme	composition	582:592	arg1	emulsions					543:551	emulsions	543:551	emulsions	543:551	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	35	theme	composition	582:592	arg1	dispersions					527:537	CNF dispersions	523:537	CNF dispersions	523:537	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	2	36	theme	ionic	317:321	arg1	surfactant					323:332	an ionic surfactant	314:332	an ionic surfactant (sodium dodecyl sulfate, SDS)	314:362	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	2	36	theme	ionic	317:321	arg1	emulsifier					376:385	emulsifier	376:385	emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF	376:478	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	8	37	theme	CNF	1496:1498	arg1	addition					1484:1491	addition	1484:1491	addition of CNF	1484:1498	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	4	38	from	transfer	888:895	arg1	system					917:922	the oil-in-water system	900:922	the oil-in-water system	900:922	At low salinity, the phase transition of emulsions containing CNF and SDS at low concentrations was controlled by molecular transfer in the oil-in-water system.
29131593	3	39	theme	SDS	603:605	arg1	concentration					607:619	SDS concentration	603:619	SDS concentration	603:619	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	2	40	theme	dodecyl	342:348	arg1	SDS					359:361	SDS	359:361	SDS	359:361	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	2	40	theme	dodecyl	342:348	arg1	sulfate					350:356	sodium dodecyl sulfate	335:356	sodium dodecyl sulfate	335:356	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	8	41	theme	high	1590:1593	arg1	stability					1595:1603	high stability	1590:1603	high stability	1590:1603	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	1	42	theme	complex	236:242	arg1	fluids					244:249	complex fluids	236:249	complex fluids	236:249	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	5	43	theme	SDS	1055:1057	arg1	concentrations					1059:1072	high CNF and SDS concentrations	1042:1072	high CNF and SDS concentrations	1042:1072	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	3	44	theme	ionic	639:643	arg1	strength					645:652	ionic strength	639:652	ionic strength	639:652	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	4	45	theme	low	767:769	arg1	salinity					771:778	low salinity	767:778	low salinity	767:778	At low salinity, the phase transition of emulsions containing CNF and SDS at low concentrations was controlled by molecular transfer in the oil-in-water system.
29131593	8	46	theme	environmentally	1536:1550	arg1	systems					1569:1575	environmentally friendly complex systems	1536:1575	environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems	1536:1690	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	3	47	theme	formulation	626:636	arg1	influence					569:577	the influence	565:577	the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior	565:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	47	theme	formulation	626:636	arg1	emulsions					543:551	emulsions	543:551	emulsions	543:551	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	47	theme	formulation	626:636	arg1	dispersions					527:537	CNF dispersions	523:537	CNF dispersions	523:537	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	48	theme	SDS	510:512	arg1	properties					496:505	The adsorption properties	481:505	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior	481:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	6	49	dep	amounts	1182:1188	arg1	the					1160:1162	the	1160:1162	the	1160:1162	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	6	49	dep	amounts	1182:1188	arg1	presence					1164:1171	presence	1164:1171	presence	1164:1171	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	8	50	theme	complex	1561:1567	arg1	systems					1569:1575	environmentally friendly complex systems	1536:1575	environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems	1536:1690	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	6	51	theme	small	1176:1180	arg1	CNF					1193:1195	CNF	1193:1195	CNF	1193:1195	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	6	51	theme	small	1176:1180	arg1	amounts					1182:1188	small amounts	1176:1188	small amounts of CNF	1176:1195	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	1	52	theme	great	167:171	arg1	stabilizer					196:205	a natural stabilizer	186:205	a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics	186:296	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	1	52	theme	great	167:171	arg1	prospects					173:181	great prospects	167:181	great prospects	167:181	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	2	53	theme	emulsions	420:428	arg1	surfactant					323:332	an ionic surfactant	314:332	an ionic surfactant (sodium dodecyl sulfate, SDS)	314:362	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	2	53	theme	emulsions	420:428	arg1	emulsifier					376:385	emulsifier	376:385	emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF	376:478	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	6	54	theme	Water-in-oil	1075:1086	arg1	emulsions					1088:1096	Water-in-oil emulsions	1075:1096	Water-in-oil emulsions	1075:1096	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	3	55	theme	CNF	523:525	arg1	dispersions					527:537	CNF dispersions	523:537	CNF dispersions	523:537	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	7	56	dep	method	1289:1294	arg1	either					1347:1352	either	1347:1352	either	1347:1352	The results revealed some subtle differences in CNF interfacial activity, depending on the method used for their isolation via fiber deconstruction, either from microfluidization or aqueous counter collision.
29131593	1	57	theme	natural	188:194	arg1	stabilizer					196:205	a natural stabilizer	186:205	a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics	186:296	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	1	57	theme	natural	188:194	arg1	prospects					173:181	great prospects	167:181	great prospects	167:181	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	3	58	theme	CNF	664:666	arg1	types					668:672	CNF types	664:672	CNF types	664:672	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	59	from	SDS	510:512	arg1	behavior					688:695	the phase behavior	678:695	the phase behavior	678:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	59	from	SDS	510:512	arg1	dispersions					527:537	CNF dispersions	523:537	CNF dispersions	523:537	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	59	from	SDS	510:512	arg1	emulsions					543:551	emulsions	543:551	emulsions	543:551	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	59	from	SDS	510:512	arg1	influence					569:577	the influence	565:577	the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior	565:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	8	60	theme	emulsion	1675:1682	arg1	systems					1684:1690	classical emulsion systems	1665:1690	classical emulsion systems	1665:1690	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	4	61	theme	phase	785:789	arg1	transition					791:800	the phase transition	781:800	the phase transition of emulsions containing CNF and SDS at low concentrations	781:858	At low salinity, the phase transition of emulsions containing CNF and SDS at low concentrations was controlled by molecular transfer in the oil-in-water system.
29131593	5	62	theme	bi-continuous	949:961	arg1	morphologies					964:975	"bi-continuous" morphologies	948:975	"bi-continuous" morphologies	948:975	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	1	63	theme	Cellulose	133:141	arg1	CNF					156:158	CNF	156:158	CNF	156:158	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	1	63	theme	Cellulose	133:141	arg1	nanofibrils					143:153	Cellulose nanofibrils	133:153	Cellulose nanofibrils (CNF)	133:159	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	6	64	theme	high	1120:1123	arg1	salinity					1125:1132	high salinity	1120:1132	high salinity	1120:1132	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	3	65	theme	systems	755:761	arg1	framework					734:742	the framework	730:742	the framework of Windsor systems	730:761	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	0	66	from	Effects	28:34	arg1	Transitions					45:55	Phase Transitions	39:55	Phase Transitions	39:55	Formulation and Composition Effects in Phase Transitions of Emulsions Costabilized by Cellulose Nanofibrils and an Ionic Surfactant.
29131593	1	67	theme	dispersions	220:230	arg1	stabilizer					196:205	a natural stabilizer	186:205	a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics	186:296	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	1	67	theme	dispersions	220:230	arg1	prospects					173:181	great prospects	167:181	great prospects	167:181	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	3	68	from	properties	496:505	arg1	dispersions					527:537	CNF dispersions	523:537	CNF dispersions	523:537	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	68	from	properties	496:505	arg1	emulsions					543:551	emulsions	543:551	emulsions	543:551	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	68	from	properties	496:505	arg1	influence					569:577	the influence	565:577	the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior	565:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	0	69	theme	Cellulose	86:94	arg1	Nanofibrils					96:106	Cellulose Nanofibrils	86:106	Cellulose Nanofibrils	86:106	Formulation and Composition Effects in Phase Transitions of Emulsions Costabilized by Cellulose Nanofibrils and an Ionic Surfactant.
29131593	4	70	theme	molecular	878:886	arg1	transfer					888:895	molecular transfer	878:895	molecular transfer in the oil-in-water system	878:922	At low salinity, the phase transition of emulsions containing CNF and SDS at low concentrations was controlled by molecular transfer in the oil-in-water system.
29131593	6	71	from	salinity	1125:1132	arg1	possible					1108:1115	possible	1108:1115	possible	1108:1115	Water-in-oil emulsions were only possible at high salinity and SDS concentrations in the presence of small amounts of CNF.
29131593	5	72	theme	CNF	1047:1049	arg1	concentrations					1059:1072	high CNF and SDS concentrations	1042:1072	high CNF and SDS concentrations	1042:1072	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	3	73	from	behavior	688:695	arg1	SDS					510:512	SDS	510:512	SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior	510:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	2	74	dep	surfactant	323:332	arg1	SDS					359:361	SDS	359:361	SDS	359:361	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	2	74	dep	surfactant	323:332	arg1	sulfate					350:356	sodium dodecyl sulfate	335:356	sodium dodecyl sulfate	335:356	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	0	75	theme	Ionic	115:119	arg1	Surfactant					121:130	an Ionic Surfactant	112:130	an Ionic Surfactant	112:130	Formulation and Composition Effects in Phase Transitions of Emulsions Costabilized by Cellulose Nanofibrils and an Ionic Surfactant.
29131593	5	76	theme	high	1005:1008	arg1	salinity					1010:1017	high salinity	1005:1017	high salinity	1005:1017	Irregular droplets and "bi-continuous" morphologies were observed at medium and high salinity for systems containing high CNF and SDS concentrations.
29131593	2	77	theme	CNF	476:478	arg1	addition					464:471	addition	464:471	addition of CNF	464:478	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	8	78	theme	emulsion	1447:1454	arg1	morphology					1456:1465	emulsion morphology	1447:1465	emulsion morphology	1447:1465	Overall, we propose that the control of emulsion morphology and stability by addition of CNF opens the possibility of developing environmentally friendly complex systems that display high stability and respond to ionic strength following the expectations of classical emulsion systems.
29131593	7	79	theme	subtle	1224:1229	arg1	differences					1231:1241	some subtle differences	1219:1241	some subtle differences in CNF interfacial activity	1219:1269	The results revealed some subtle differences in CNF interfacial activity, depending on the method used for their isolation via fiber deconstruction, either from microfluidization or aqueous counter collision.
29131593	3	80	dep	formulation	626:636	arg1	types					668:672	CNF types	664:672	CNF types	664:672	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	80	dep	formulation	626:636	arg1	oil					655:657	oil	655:657	oil	655:657	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	80	dep	formulation	626:636	arg1	strength					645:652	ionic strength	639:652	ionic strength	639:652	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	0	81	theme	Phase	39:43	arg1	Transitions					45:55	Phase Transitions	39:55	Phase Transitions	39:55	Formulation and Composition Effects in Phase Transitions of Emulsions Costabilized by Cellulose Nanofibrils and an Ionic Surfactant.
29131593	7	82	dep	microfluidization	1359:1375	arg1	collision					1396:1404	counter collision	1388:1404	counter collision	1388:1404	The results revealed some subtle differences in CNF interfacial activity, depending on the method used for their isolation via fiber deconstruction, either from microfluidization or aqueous counter collision.
29131593	4	83	theme	oil-in-water	904:915	arg1	system					917:922	the oil-in-water system	900:922	the oil-in-water system	900:922	At low salinity, the phase transition of emulsions containing CNF and SDS at low concentrations was controlled by molecular transfer in the oil-in-water system.
29131593	2	84	theme	sodium	335:340	arg1	SDS					359:361	SDS	359:361	SDS	359:361	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	2	84	theme	sodium	335:340	arg1	sulfate					350:356	sodium dodecyl sulfate	335:356	sodium dodecyl sulfate	335:356	In this study, an ionic surfactant (sodium dodecyl sulfate, SDS) was used as emulsifier of oil-in-water and water-in-oil emulsions that were further costabilized by addition of CNF.
29131593	3	85	dep	composition	582:592	arg1	CNF					595:597	CNF	595:597	CNF	595:597	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	3	85	dep	composition	582:592	arg1	concentration					607:619	SDS concentration	603:619	SDS concentration	603:619	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	7	86	from	differences	1231:1241	arg1	activity					1262:1269	CNF interfacial activity	1246:1269	CNF interfacial activity	1246:1269	The results revealed some subtle differences in CNF interfacial activity, depending on the method used for their isolation via fiber deconstruction, either from microfluidization or aqueous counter collision.
29131593	3	87	theme	adsorption	485:494	arg1	properties					496:505	The adsorption properties	481:505	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior	481:695	The adsorption properties of SDS in both, CNF dispersions and emulsions, as well as the influence of composition (CNF and SDS concentration) and formulation (ionic strength, oil, and CNF types) on the phase behavior were elucidated and described in the framework of Windsor systems.
29131593	1	88	theme	fluids	244:249	arg1	stabilizer					196:205	a natural stabilizer	186:205	a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics	186:296	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
29131593	1	88	theme	fluids	244:249	arg1	prospects					173:181	great prospects	167:181	great prospects	167:181	Cellulose nanofibrils (CNF) offer great prospects as a natural stabilizer of colloidal dispersions and complex fluids for application in food, pharma, and cosmetics.
26523578	3	0	theme	acidosis	837:844	arg1	index					846:850	the acidosis index	833:850	the acidosis index with a root mean square error (RMSE) of 4.46%	833:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	1	1	theme	culture	210:216	arg1	incubation					218:227	the in vitro batch culture incubation	191:227	the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices	191:343	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	8	2	dep	model	1681:1685	arg1	calculated					1688:1697	calculated	1688:1697	calculated using in situ starch degradation	1688:1730	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	3	3	theme	square	869:874	arg1	RMSE					883:886	RMSE	883:886	RMSE	883:886	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	3	3	theme	square	869:874	arg1	error					876:880	a root mean square error	857:880	a root mean square error (RMSE) of 4.46%	857:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	5	4	dep	in	1135:1136	arg1	vitro					1138:1142	vitro	1138:1142	vitro	1138:1142	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	3	5	theme	root	859:862	arg1	RMSE					883:886	RMSE	883:886	RMSE	883:886	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	3	5	theme	root	859:862	arg1	error					876:880	a root mean square error	857:880	a root mean square error (RMSE) of 4.46%	857:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	8	6	theme	samples	1586:1592	arg1	Ranking					1575:1581	Ranking	1575:1581	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation)	1575:1731	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	5	7	theme	acidosis	1203:1210	arg1	index					1212:1216	the acidosis index	1199:1216	the acidosis index with an RMSE of 8.30%	1199:1238	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	9	8	theme	correlation	2022:2032	arg1	analysis					2034:2041	a correlation analysis	2020:2041	a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001)	2020:2185	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	8	9	dep	Black	1668:1672	arg1	2008					1675:1678	2008	1675:1678	2008	1675:1678	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	7	10	from	evident	1467:1473	arg1	2					1478:1478	2	1478:1478	2	1478:1478	Significant ( < 0.001) mean bias was evident in 2 of the datasets, for which the DMD6 model underpredicted the acidosis index by 46.9 and 5.73%.
26523578	7	10	from	evident	1467:1473	arg1	datasets					1487:1494	the datasets	1483:1494	the datasets	1483:1494	Significant ( < 0.001) mean bias was evident in 2 of the datasets, for which the DMD6 model underpredicted the acidosis index by 46.9 and 5.73%.
26523578	4	11	theme	previous	962:969	arg1	studies					985:991	previous batch culture studies	962:991	previous batch culture studies using full-strength [100%] buffer	962:1025	To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer.
26523578	6	12	theme	independent	1298:1308	arg1	datasets					1310:1317	3 independent datasets	1296:1317	3 independent datasets	1296:1317	When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index.
26523578	1	13	theme	acidosis	153:160	arg1	potential					162:170	the acidosis potential	149:170	the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices	149:343	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	2	14	theme	several	469:475	arg1	components					477:486	several components	469:486	several components of grain chemical composition	469:516	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	7	15	theme	datasets	1487:1494	arg1	2					1478:1478	2	1478:1478	2	1478:1478	Significant ( < 0.001) mean bias was evident in 2 of the datasets, for which the DMD6 model underpredicted the acidosis index by 46.9 and 5.73%.
26523578	7	15	theme	datasets	1487:1494	arg1	datasets					1487:1494	the datasets	1483:1494	the datasets	1483:1494	Significant ( < 0.001) mean bias was evident in 2 of the datasets, for which the DMD6 model underpredicted the acidosis index by 46.9 and 5.73%.
26523578	7	16	from	2	1478:1478	arg1	evident					1467:1473	evident	1467:1473	evident	1467:1473	Significant ( < 0.001) mean bias was evident in 2 of the datasets, for which the DMD6 model underpredicted the acidosis index by 46.9 and 5.73%.
26523578	7	17	theme	DMD6	1511:1514	arg1	model					1516:1520	the DMD6 model	1507:1520	the DMD6 model	1507:1520	Significant ( < 0.001) mean bias was evident in 2 of the datasets, for which the DMD6 model underpredicted the acidosis index by 46.9 and 5.73%.
26523578	8	18	theme	diverse	1608:1614	arg1	dataset					1628:1634	the most diverse independent dataset	1599:1634	the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation)	1599:1731	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	6	19	from	%	1377:1377	arg1	index					1423:1427	the calculated acidosis index	1399:1427	the calculated acidosis index	1399:1427	When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index.
26523578	2	20	theme	chemical	497:504	arg1	composition					506:516	grain chemical composition	491:516	grain chemical composition	491:516	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	0	21	from	risk	54:57	arg1	cattle					93:98	finishing beef cattle	78:98	finishing beef cattle	78:98	Evaluation of in vitro models for predicting acidosis risk of barley grain in finishing beef cattle.
26523578	4	22	theme	[100	1013:1016	arg1	%					1017:1017	%	1017:1017	%	1017:1017	To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer.
26523578	9	23	theme	reduced-strength	1859:1874	arg1	model					1883:1887	the reduced-strength buffer model	1855:1887	the reduced-strength buffer model	1855:1887	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	3	24	theme	reduced-strength	742:757	arg1	buffer					765:770	reduced-strength (20%) buffer	742:770	reduced-strength (20%) buffer	742:770	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	8	25	dep	in	1705:1706	arg1	situ					1708:1711	situ	1708:1711	situ	1708:1711	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	2	26	theme	in	430:431	arg1	analyses					451:458	in situ and in vitro analyses	430:458	in situ and in vitro analyses	430:458	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	6	27	theme	calculated	1403:1412	arg1	index					1423:1427	the calculated acidosis index	1399:1427	the calculated acidosis index	1399:1427	When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index.
26523578	4	28	theme	culture	977:983	arg1	studies					985:991	previous batch culture studies	962:991	previous batch culture studies using full-strength [100%] buffer	962:1025	To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer.
26523578	9	29	from	similarities	1919:1930	arg1	index					1948:1952	the acidosis index	1935:1952	the acidosis index ranking of barley samples by the models	1935:1992	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	0	30	theme	barley	62:67	arg1	grain					69:73	barley grain	62:73	barley grain	62:73	Evaluation of in vitro models for predicting acidosis risk of barley grain in finishing beef cattle.
26523578	1	31	theme	processing	284:293	arg1	method					295:300	processing method	284:300	processing method	284:300	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	2	32	dep	index	390:394	arg1	calculated					397:406	calculated	397:406	calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition	397:516	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	10	33	theme	acidosis	2308:2315	arg1	risk					2317:2320	acidosis risk	2308:2320	acidosis risk	2308:2320	Results suggest that our model, which is based on a reduced-strength buffer in vitro DMD6, has the potential to predict acidosis risk and can rank barley samples based on their acidotic risk.
26523578	9	34	theme	Australian	2073:2082	arg1	model					2084:2088	the Australian model	2069:2088	the Australian model	2069:2088	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	9	35	used	used	1893:1896	arg2	model					1883:1887	the reduced-strength buffer model	1855:1887	the reduced-strength buffer model	1855:1887	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	9	36	theme	buffer	2133:2138	arg1	model					2145:2149	the reduced-strength buffer DMD6 model	2112:2149	the reduced-strength buffer DMD6 model	2112:2149	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	6	37	theme	variation	1386:1394	arg1	%					1377:1377	20.1, 28.5, and 30.2%	1357:1377	20.1, 28.5, and 30.2% of the variation in the calculated acidosis index	1357:1427	When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index.
26523578	6	37	theme	variation	1386:1394	arg1	variation					1386:1394	the variation	1382:1394	the variation in the calculated acidosis index	1382:1427	When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index.
26523578	3	38	theme	batch	779:783	arg1	culture					785:791	the batch culture	775:791	the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%	775:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	4	39	theme	culture	1056:1062	arg1	study					1064:1068	another batch culture study	1042:1068	another batch culture study	1042:1068	To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer.
26523578	0	40	theme	beef	88:91	arg1	cattle					93:98	finishing beef cattle	78:98	finishing beef cattle	78:98	Evaluation of in vitro models for predicting acidosis risk of barley grain in finishing beef cattle.
26523578	7	41	theme	Significant	1430:1440	arg1	bias					1458:1461	Significant ( < 0.001) mean bias	1430:1461	Significant ( < 0.001) mean bias	1430:1461	Significant ( < 0.001) mean bias was evident in 2 of the datasets, for which the DMD6 model underpredicted the acidosis index by 46.9 and 5.73%.
26523578	2	42	theme	acidosis	381:388	arg1	index					390:394	the acidosis index	377:394	the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis	377:647	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	10	43	dep	in	2264:2265	arg1	vitro					2267:2271	vitro	2267:2271	vitro	2267:2271	Results suggest that our model, which is based on a reduced-strength buffer in vitro DMD6, has the potential to predict acidosis risk and can rank barley samples based on their acidotic risk.
26523578	9	44	theme	acidosis	2152:2159	arg1	index					2161:2165	acidosis index	2152:2165	acidosis index	2152:2165	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	8	45	from	Ranking	1575:1581	arg1	dataset					1628:1634	the most diverse independent dataset	1599:1634	the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation)	1599:1731	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	11	46	theme	further	2422:2428	arg1	refinement					2430:2439	further refinement	2422:2439	further refinement	2422:2439	Nonetheless, the model would benefit from further refinement by expanding the database.
26523578	5	47	theme	in	1135:1136	arg1	DMD6-FS					1150:1156	in vitro DMD6 (DMD6-FS)	1135:1157	in vitro DMD6 (DMD6-FS)	1135:1157	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	0	48	theme	in	14:15	arg1	models					23:28	in vitro models	14:28	in vitro models	14:28	Evaluation of in vitro models for predicting acidosis risk of barley grain in finishing beef cattle.
26523578	8	49	theme	rank	1798:1801	arg1	correlation					1803:1813	rank correlation	1798:1813	rank correlation	1798:1813	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	5	50	theme	DMD6	1144:1147	arg1	DMD6-FS					1150:1156	in vitro DMD6 (DMD6-FS)	1135:1157	in vitro DMD6 (DMD6-FS)	1135:1157	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	0	51	theme	models	23:28	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of in vitro models for predicting acidosis risk of barley grain in finishing beef cattle.	0:99	Evaluation of in vitro models for predicting acidosis risk of barley grain in finishing beef cattle.
26523578	1	52	theme	bulk	254:257	arg1	density					259:265	bulk density	254:265	bulk density	254:265	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	2	53	dep	in	442:443	arg1	vitro					445:449	vitro	445:449	vitro	445:449	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	3	54	theme	variation	820:828	arg1	variation					820:828	the variation	816:828	the variation in the acidosis index with a root mean square error (RMSE) of 4.46%	816:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	3	54	theme	variation	820:828	arg1	%					811:811	90.5%	807:811	90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%	807:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	5	55	from	variation	1186:1194	arg1	index					1212:1216	the acidosis index	1199:1216	the acidosis index with an RMSE of 8.30%	1199:1238	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	5	56	theme	variation	1186:1194	arg1	%					1177:1177	66.5%	1173:1177	66.5% of the variation in the acidosis index with an RMSE of 8.30%	1173:1238	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	5	56	theme	variation	1186:1194	arg1	variation					1186:1194	the variation	1182:1194	the variation in the acidosis index with an RMSE of 8.30%	1182:1238	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	2	57	from	use	546:548	arg1	industry					562:569	the feed industry	553:569	the feed industry	553:569	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	10	58	theme	in	2264:2265	arg1	DMD6					2273:2276	a reduced-strength buffer in vitro DMD6	2238:2276	a reduced-strength buffer in vitro DMD6	2238:2276	Results suggest that our model, which is based on a reduced-strength buffer in vitro DMD6, has the potential to predict acidosis risk and can rank barley samples based on their acidotic risk.
26523578	0	59	dep	in	14:15	arg1	vitro					17:21	vitro	17:21	vitro	17:21	Evaluation of in vitro models for predicting acidosis risk of barley grain in finishing beef cattle.
26523578	7	60	theme	acidosis	1541:1548	arg1	index					1550:1554	the acidosis index	1537:1554	the acidosis index	1537:1554	Significant ( < 0.001) mean bias was evident in 2 of the datasets, for which the DMD6 model underpredicted the acidosis index by 46.9 and 5.73%.
26523578	5	61	from	index	1212:1216	arg1	%					1177:1177	66.5%	1173:1177	66.5% of the variation in the acidosis index with an RMSE of 8.30%	1173:1238	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	5	61	from	index	1212:1216	arg1	variation					1186:1194	the variation	1182:1194	the variation in the acidosis index with an RMSE of 8.30%	1182:1238	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	8	62	from	dataset	1628:1634	arg1	Ranking					1575:1581	Ranking	1575:1581	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation)	1575:1731	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	8	62	from	dataset	1628:1634	arg1	samples					1586:1592	samples	1586:1592	samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation)	1586:1731	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	9	63	theme	barley	1965:1970	arg1	samples					1972:1978	barley samples	1965:1978	barley samples	1965:1978	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	10	64	theme	reduced-strength	2240:2255	arg1	DMD6					2273:2276	a reduced-strength buffer in vitro DMD6	2238:2276	a reduced-strength buffer in vitro DMD6	2238:2276	Results suggest that our model, which is based on a reduced-strength buffer in vitro DMD6, has the potential to predict acidosis risk and can rank barley samples based on their acidotic risk.
26523578	3	65	with	index	846:850	arg1	RMSE					883:886	RMSE	883:886	RMSE	883:886	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	3	65	with	index	846:850	arg1	error					876:880	a root mean square error	857:880	a root mean square error (RMSE) of 4.46%	857:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	1	66	theme	batch	204:208	arg1	incubation					218:227	the in vitro batch culture incubation	191:227	the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices	191:343	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	3	67	theme	mean	864:867	arg1	RMSE					883:886	RMSE	883:886	RMSE	883:886	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	3	67	theme	mean	864:867	arg1	error					876:880	a root mean square error	857:880	a root mean square error (RMSE) of 4.46%	857:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	2	68	theme	ruminal	632:638	arg1	acidosis					640:647	ruminal acidosis	632:647	ruminal acidosis	632:647	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	2	69	theme	composition	506:516	arg1	components					477:486	several components	469:486	several components of grain chemical composition	469:516	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	9	70	dep	0.67	2172:2175	arg1	<					2178:2178	< 0.001	2178:2184	< 0.001	2178:2184	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	5	71	theme	%	1238:1238	arg1	RMSE					1226:1229	an RMSE	1223:1229	an RMSE of 8.30%	1223:1238	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	8	72	dep	Spearman	1787:1794	arg1	negative					1819:1826	negative	1819:1826	negative	1819:1826	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	6	73	from	variation	1386:1394	arg1	index					1423:1427	the calculated acidosis index	1399:1427	the calculated acidosis index	1399:1427	When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index.
26523578	2	74	theme	index	390:394	arg1	model					350:354	The model	346:354	The model	346:354	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	2	74	theme	index	390:394	arg1	adaptation					363:372	an adaptation	360:372	an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis	360:647	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	4	75	theme	batch	971:975	arg1	studies					985:991	previous batch culture studies	962:991	previous batch culture studies using full-strength [100%] buffer	962:1025	To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer.
26523578	9	76	theme	analysis	2034:2041	arg1	result					2010:2015	the result	2006:2015	the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001)	2006:2185	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	4	77	theme	independent	927:937	arg1	datasets					939:946	independent datasets	927:946	independent datasets (derived from previous batch culture studies using full-strength [100%] buffer)	927:1026	To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer.
26523578	3	78	theme	independent	657:667	arg1	variables					669:677	the independent variables	653:677	the independent variables considered	653:688	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	1	79	dep	in	195:196	arg1	vitro					198:202	vitro	198:202	vitro	198:202	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	1	80	theme	agronomic	325:333	arg1	practices					335:343	agronomic practices	325:343	agronomic practices	325:343	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	1	81	theme	barley	175:180	arg1	potential					162:170	the acidosis potential	149:170	the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices	149:343	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	2	82	theme	analyses	451:458	arg1	combination					415:425	a combination	413:425	a combination of in situ and in vitro analyses	413:458	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	4	83	theme	%	1017:1017	arg1	buffer					1020:1025	full-strength [100%] buffer	999:1025	full-strength [100%] buffer	999:1025	To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer.
26523578	3	84	theme	DM	691:692	arg1	disappearance					694:706	DM disappearance	691:706	DM disappearance at 6 h of incubation (DMD6)	691:734	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	8	85	theme	DMD6-FS	1646:1652	arg1	model					1654:1658	the DMD6-FS model	1642:1658	the DMD6-FS model	1642:1658	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	8	86	dep	=	1831:1831	arg1	-0.30					1833:1837	-0.30	1833:1837	-0.30	1833:1837	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	2	87	theme	grain	491:495	arg1	composition					506:516	grain chemical composition	491:516	grain chemical composition	491:516	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	3	88	from	%	811:811	arg1	index					846:850	the acidosis index	833:850	the acidosis index with a root mean square error (RMSE) of 4.46%	833:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	9	89	theme	buffer	1876:1881	arg1	model					1883:1887	the reduced-strength buffer model	1855:1887	the reduced-strength buffer model	1855:1887	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	6	90	theme	acidosis	1414:1421	arg1	index					1423:1427	the calculated acidosis index	1399:1427	the calculated acidosis index	1399:1427	When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index.
26523578	2	91	theme	acidosis	640:647	arg1	risk					624:627	the risk	620:627	the risk of ruminal acidosis	620:647	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	5	92	theme	buffer	1116:1121	arg1	model					1123:1127	The full-strength buffer model	1098:1127	The full-strength buffer model using in vitro DMD6 (DMD6-FS)	1098:1157	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	8	93	theme	Black	1668:1672	arg1	model					1681:1685	the Black (2008) model	1664:1685	the Black (2008) model (calculated using in situ starch degradation)	1664:1731	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	10	94	contain	has	2279:2281	arg2	potential					2287:2295	the potential to predict acidosis risk	2283:2320	the potential to predict acidosis risk	2283:2320	Results suggest that our model, which is based on a reduced-strength buffer in vitro DMD6, has the potential to predict acidosis risk and can rank barley samples based on their acidotic risk.
26523578	10	94	contain	has	2279:2281	arg1	model					2213:2217	our model	2209:2217	our model	2209:2217	Results suggest that our model, which is based on a reduced-strength buffer in vitro DMD6, has the potential to predict acidosis risk and can rank barley samples based on their acidotic risk.
26523578	4	95	theme	full-strength	999:1011	arg1	%					1017:1017	%	1017:1017	%	1017:1017	To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer.
26523578	0	96	theme	grain	69:73	arg1	risk					54:57	acidosis risk	45:57	acidosis risk of barley grain in finishing beef cattle	45:98	Evaluation of in vitro models for predicting acidosis risk of barley grain in finishing beef cattle.
26523578	3	97	theme	%	896:896	arg1	RMSE					883:886	RMSE	883:886	RMSE	883:886	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	3	97	theme	%	896:896	arg1	error					876:880	a root mean square error	857:880	a root mean square error (RMSE) of 4.46%	857:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	4	98	theme	batch	1050:1054	arg1	study					1064:1068	another batch culture study	1042:1068	another batch culture study	1042:1068	To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer.
26523578	0	99	theme	finishing	78:86	arg1	cattle					93:98	finishing beef cattle	78:98	finishing beef cattle	78:98	Evaluation of in vitro models for predicting acidosis risk of barley grain in finishing beef cattle.
26523578	10	100	theme	buffer	2257:2262	arg1	DMD6					2273:2276	a reduced-strength buffer in vitro DMD6	2238:2276	a reduced-strength buffer in vitro DMD6	2238:2276	Results suggest that our model, which is based on a reduced-strength buffer in vitro DMD6, has the potential to predict acidosis risk and can rank barley samples based on their acidotic risk.
26523578	9	101	theme	reduced-strength	2116:2131	arg1	model					2145:2149	the reduced-strength buffer DMD6 model	2112:2149	the reduced-strength buffer DMD6 model	2112:2149	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	7	102	theme	mean	1453:1456	arg1	bias					1458:1461	Significant ( < 0.001) mean bias	1430:1461	Significant ( < 0.001) mean bias	1430:1461	Significant ( < 0.001) mean bias was evident in 2 of the datasets, for which the DMD6 model underpredicted the acidosis index by 46.9 and 5.73%.
26523578	2	103	theme	in	442:443	arg1	analyses					451:458	in situ and in vitro analyses	430:458	in situ and in vitro analyses	430:458	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	2	104	theme	feed	557:560	arg1	industry					562:569	the feed industry	553:569	the feed industry	553:569	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	10	105	theme	acidotic	2365:2372	arg1	risk					2374:2377	their acidotic risk	2359:2377	their acidotic risk	2359:2377	Results suggest that our model, which is based on a reduced-strength buffer in vitro DMD6, has the potential to predict acidosis risk and can rank barley samples based on their acidotic risk.
26523578	3	106	theme	incubation	718:727	arg1	h					713:713	6 h	711:713	6 h of incubation (DMD6)	711:734	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	1	107	theme	growing	303:309	arg1	location					311:318	growing location	303:318	growing location	303:318	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	9	108	theme	DMD6	2140:2143	arg1	model					2145:2149	the reduced-strength buffer DMD6 model	2112:2149	the reduced-strength buffer DMD6 model	2112:2149	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	5	109	from	%	1177:1177	arg1	index					1212:1216	the acidosis index	1199:1216	the acidosis index with an RMSE of 8.30%	1199:1238	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	10	110	theme	barley	2335:2340	arg1	samples					2342:2348	barley samples	2335:2348	barley samples based on their acidotic risk	2335:2377	Results suggest that our model, which is based on a reduced-strength buffer in vitro DMD6, has the potential to predict acidosis risk and can rank barley samples based on their acidotic risk.
26523578	8	111	theme	in	1705:1706	arg1	degradation					1720:1730	in situ starch degradation	1705:1730	in situ starch degradation	1705:1730	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	3	112	from	index	846:850	arg1	variation					820:828	the variation	816:828	the variation in the acidosis index with a root mean square error (RMSE) of 4.46%	816:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	3	112	from	index	846:850	arg1	%					811:811	90.5%	807:811	90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%	807:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	9	113	theme	acidosis	1939:1946	arg1	index					1948:1952	the acidosis index	1935:1952	the acidosis index ranking of barley samples by the models	1935:1992	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	8	114	theme	starch	1713:1718	arg1	degradation					1720:1730	in situ starch degradation	1705:1730	in situ starch degradation	1705:1730	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	1	115	theme	samples	235:241	arg1	incubation					218:227	the in vitro batch culture incubation	191:227	the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices	191:343	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	4	116	attach	derived	949:955	arg1	studies					985:991	previous batch culture studies	962:991	previous batch culture studies using full-strength [100%] buffer	962:1025	To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer.
26523578	4	116	attach	derived	949:955	arg2	datasets					939:946	independent datasets	927:946	independent datasets (derived from previous batch culture studies using full-strength [100%] buffer)	927:1026	To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer.
26523578	9	117	dep	predicted	2095:2103	arg1	using					2106:2110	using	2106:2110	using the reduced-strength buffer DMD6 model	2106:2149	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	9	118	dep	calculated	2051:2060	arg1	using					2063:2067	using	2063:2067	using the Australian model	2063:2088	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	9	118	dep	calculated	2051:2060	arg1	index					2161:2165	acidosis index	2152:2165	acidosis index	2152:2165	When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001).
26523578	5	119	with	index	1212:1216	arg1	RMSE					1226:1229	an RMSE	1223:1229	an RMSE of 8.30%	1223:1238	The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%.
26523578	3	120	from	variation	820:828	arg1	index					846:850	the acidosis index	833:850	the acidosis index with a root mean square error (RMSE) of 4.46%	833:896	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	8	121	dep	diverse	1608:1614	arg1	independent					1616:1626	independent	1616:1626	independent	1616:1626	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	1	122	theme	in	195:196	arg1	incubation					218:227	the in vitro batch culture incubation	191:227	the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices	191:343	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	8	123	dep	=	1840:1840	arg1	ρ					1829:1829	ρ	1829:1829	ρ = -0.30	1829:1837	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	0	124	theme	acidosis	45:52	arg1	risk					54:57	acidosis risk	45:57	acidosis risk of barley grain in finishing beef cattle	45:98	Evaluation of in vitro models for predicting acidosis risk of barley grain in finishing beef cattle.
26523578	6	125	from	index	1423:1427	arg1	%					1377:1377	20.1, 28.5, and 30.2%	1357:1377	20.1, 28.5, and 30.2% of the variation in the calculated acidosis index	1357:1427	When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index.
26523578	6	125	from	index	1423:1427	arg1	variation					1386:1394	the variation	1382:1394	the variation in the calculated acidosis index	1382:1427	When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index.
26523578	1	126	theme	starch	268:273	arg1	content					275:281	starch content	268:281	starch content	268:281	Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices.
26523578	2	127	dep	in	430:431	arg1	situ					433:436	situ	433:436	situ	433:436	The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis.
26523578	3	128	from	h	713:713	arg1	disappearance					694:706	DM disappearance	691:706	DM disappearance at 6 h of incubation (DMD6)	691:734	Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%.
26523578	7	129	dep	Significant	1430:1440	arg1	<					1444:1444	< 0.001	1444:1450	< 0.001	1444:1450	Significant ( < 0.001) mean bias was evident in 2 of the datasets, for which the DMD6 model underpredicted the acidosis index by 46.9 and 5.73%.
26523578	6	130	theme	buffer	1268:1273	arg1	model					1275:1279	the new full-strength buffer model	1246:1279	the new full-strength buffer model	1246:1279	When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index.
26523578	8	131	theme	=	1831:1831	arg1	ρ					1829:1829	ρ	1829:1829	ρ = -0.30	1829:1837	Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059).
26523578	6	132	theme	new	1250:1252	arg1	model					1275:1279	the new full-strength buffer model	1246:1279	the new full-strength buffer model	1246:1279	When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index.
25359676	0	0	theme	porcine	91:97	arg1	lipase					110:115	porcine pancreatic lipase	91:115	porcine pancreatic lipase	91:115	Synthesis and characterization of chitosan/TiO2 composite beads for improving stability of porcine pancreatic lipase.
25359676	6	1	theme	optimum	835:841	arg1	values					846:851	The optimum pH values	831:851	The optimum pH values for the free lipase and immobilized lipase	831:894	The optimum pH values for the free lipase and immobilized lipase were found to be 6.5 and 7.5, respectively.
25359676	6	1	theme	optimum	835:841	arg1	6.5					913:915	6.5	913:915	6.5	913:915	The optimum pH values for the free lipase and immobilized lipase were found to be 6.5 and 7.5, respectively.
25359676	7	2	theme	45	1017:1018	arg1	%					1020:1020	%	1020:1020	%	1020:1020	The thermal stability of immobilized lipase was evaluated, and it maintained 45 % activity at 70 °C. But, at this temperature, soluble lipase protected only 15 % activity.
25359676	5	3	theme	large	819:823	arg1	area					825:828	a large area	817:828	a large area	817:828	The temperature profile of the immobilized lipase was spread over a large area.
25359676	8	4	theme	infrared	1348:1355	arg1	analysis					1370:1377	infrared spectroscopy analysis	1348:1377	infrared spectroscopy analysis (ATR-FTIR)	1348:1388	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	7	5	theme	%	1020:1020	arg1	activity					1022:1029	45 % activity	1017:1029	45 % activity	1017:1029	The thermal stability of immobilized lipase was evaluated, and it maintained 45 % activity at 70 °C. But, at this temperature, soluble lipase protected only 15 % activity.
25359676	1	6	theme	stability	163:171	arg1	properties					173:182	stability properties	163:182	stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3)	163:249	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	8	7	dep	analysis	1370:1377	arg1	ATR-FTIR					1380:1387	ATR-FTIR	1380:1387	ATR-FTIR	1380:1387	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	0	8	theme	lipase	110:115	arg1	stability					78:86	stability	78:86	stability of porcine pancreatic lipase	78:115	Synthesis and characterization of chitosan/TiO2 composite beads for improving stability of porcine pancreatic lipase.
25359676	9	9	theme	industrial	1477:1486	arg1	usage					1488:1492	the industrial usage	1473:1492	the industrial usage especially production of biodiesel and dairy products	1473:1546	The significance of this study is improving of stability properties of lipase for the industrial usage especially production of biodiesel and dairy products.
25359676	9	10	theme	biodiesel	1519:1527	arg1	production					1505:1514	the industrial usage especially production	1473:1514	the industrial usage especially production of biodiesel and dairy products	1473:1546	The significance of this study is improving of stability properties of lipase for the industrial usage especially production of biodiesel and dairy products.
25359676	3	11	theme	optimum	492:498	arg1	3.0-10.0					504:511	3.0-10.0	504:511	3.0-10.0	504:511	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	3	11	theme	optimum	492:498	arg1	pH					500:501	optimum pH	492:501	optimum pH (3.0-10.0)	492:512	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	2	12	theme	immobilization	307:320	arg1	concentration					353:365	initial enzyme concentration	338:365	initial enzyme concentration (0.5-2 mg/ml)	338:379	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	2	12	theme	immobilization	307:320	arg1	parameters					322:331	The immobilization parameters	303:331	The immobilization parameters	303:331	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	9	13	theme	stability	1438:1446	arg1	properties					1448:1457	stability properties	1438:1457	stability properties of lipase	1438:1467	The significance of this study is improving of stability properties of lipase for the industrial usage especially production of biodiesel and dairy products.
25359676	1	14	theme	chitosan/TiO2	272:284	arg1	beads					296:300	chitosan/TiO2 composite beads	272:300	chitosan/TiO2 composite beads	272:300	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	0	15	theme	pancreatic	99:108	arg1	lipase					110:115	porcine pancreatic lipase	91:115	porcine pancreatic lipase	91:115	Synthesis and characterization of chitosan/TiO2 composite beads for improving stability of porcine pancreatic lipase.
25359676	4	16	theme	optimum	687:693	arg1	temperatures					695:706	The optimum temperatures	683:706	The optimum temperatures of free and immobilized lipase	683:737	The optimum temperatures of free and immobilized lipase were 30 °C.
25359676	4	16	theme	optimum	687:693	arg1	°C					747:748	30 °C	744:748	30 °C	744:748	The optimum temperatures of free and immobilized lipase were 30 °C.
25359676	1	17	theme	composite	286:294	arg1	beads					296:300	chitosan/TiO2 composite beads	272:300	chitosan/TiO2 composite beads	272:300	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	3	18	theme	immobilized	656:666	arg1	system					675:680	immobilized lipase system	656:680	immobilized lipase system	656:680	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	9	19	theme	dairy	1533:1537	arg1	products					1539:1546	dairy products	1533:1546	dairy products	1533:1546	The significance of this study is improving of stability properties of lipase for the industrial usage especially production of biodiesel and dairy products.
25359676	3	20	theme	optimum	460:466	arg1	temperature					468:478	The optimum temperature	456:478	The optimum temperature (20-60 °C)	456:489	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	3	20	theme	optimum	460:466	arg1	°C					487:488	20-60 °C	481:488	20-60 °C	481:488	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	4	21	theme	free	711:714	arg1	lipase					732:737	free and immobilized lipase	711:737	free and immobilized lipase	711:737	The optimum temperatures of free and immobilized lipase were 30 °C.
25359676	5	22	theme	immobilized	782:792	arg1	lipase					794:799	the immobilized lipase	778:799	the immobilized lipase	778:799	The temperature profile of the immobilized lipase was spread over a large area.
25359676	9	23	theme	products	1539:1546	arg1	production					1505:1514	the industrial usage especially production	1473:1514	the industrial usage especially production of biodiesel and dairy products	1473:1546	The significance of this study is improving of stability properties of lipase for the industrial usage especially production of biodiesel and dairy products.
25359676	9	24	theme	study	1416:1420	arg1	improving					1425:1433	improving	1425:1433	improving	1425:1433	The significance of this study is improving of stability properties of lipase for the industrial usage especially production of biodiesel and dairy products.
25359676	9	24	theme	study	1416:1420	arg1	significance					1395:1406	The significance	1391:1406	The significance of this study	1391:1420	The significance of this study is improving of stability properties of lipase for the industrial usage especially production of biodiesel and dairy products.
25359676	8	25	theme	total	1313:1317	arg1	Fourier					1330:1336	attenuated total reflection Fourier	1302:1336	attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR)	1302:1388	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	5	26	theme	lipase	794:799	arg1	profile					767:773	The temperature profile	751:773	The temperature profile of the immobilized lipase	751:799	The temperature profile of the immobilized lipase was spread over a large area.
25359676	2	27	theme	enzyme	346:351	arg1	concentration					353:365	initial enzyme concentration	338:365	initial enzyme concentration (0.5-2 mg/ml)	338:379	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	2	27	theme	enzyme	346:351	arg1	mg/ml					374:378	0.5-2 mg/ml	368:378	0.5-2 mg/ml	368:378	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	2	27	theme	enzyme	346:351	arg1	parameters					322:331	The immobilization parameters	303:331	The immobilization parameters	303:331	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	1	28	theme	porcine	187:193	arg1	lipase					206:211	porcine pancreatic lipase	187:211	porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3)	187:249	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	2	29	theme	%	448:448	arg1	v/v					450:452	1-4 % v/v	444:452	1-4 % v/v	444:452	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	2	29	theme	%	448:448	arg1	concentration					429:441	glutaraldehyde concentration	414:441	glutaraldehyde concentration (1-4 % v/v)	414:453	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	2	30	theme	initial	338:344	arg1	concentration					353:365	initial enzyme concentration	338:365	initial enzyme concentration (0.5-2 mg/ml)	338:379	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	2	30	theme	initial	338:344	arg1	mg/ml					374:378	0.5-2 mg/ml	368:378	0.5-2 mg/ml	368:378	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	2	30	theme	initial	338:344	arg1	parameters					322:331	The immobilization parameters	303:331	The immobilization parameters	303:331	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	1	31	theme	pancreatic	195:204	arg1	lipase					206:211	porcine pancreatic lipase	187:211	porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3)	187:249	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	6	32	theme	immobilized	877:887	arg1	lipase					889:894	immobilized lipase	877:894	immobilized lipase	877:894	The optimum pH values for the free lipase and immobilized lipase were found to be 6.5 and 7.5, respectively.
25359676	7	33	theme	immobilized	965:975	arg1	lipase					977:982	immobilized lipase	965:982	immobilized lipase	965:982	The thermal stability of immobilized lipase was evaluated, and it maintained 45 % activity at 70 °C. But, at this temperature, soluble lipase protected only 15 % activity.
25359676	3	34	theme	kinetic	515:521	arg1	parameters					523:532	kinetic parameters	515:532	kinetic parameters	515:532	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	8	35	theme	spectroscopy	1357:1368	arg1	analysis					1370:1377	infrared spectroscopy analysis	1348:1377	infrared spectroscopy analysis (ATR-FTIR)	1348:1388	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	1	36	theme	lipase	206:211	arg1	properties					173:182	stability properties	163:182	stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3)	163:249	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	3	37	theme	thermal	535:541	arg1	stability					543:551	thermal stability	535:551	thermal stability (4-70 °C)	535:561	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	3	37	theme	thermal	535:541	arg1	°C					559:560	4-70 °C	554:560	4-70 °C	554:560	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	3	38	theme	lipase	668:673	arg1	system					675:680	immobilized lipase system	656:680	immobilized lipase system	656:680	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	7	39	theme	lipase	977:982	arg1	stability					952:960	The thermal stability	940:960	The thermal stability of immobilized lipase	940:982	The thermal stability of immobilized lipase was evaluated, and it maintained 45 % activity at 70 °C. But, at this temperature, soluble lipase protected only 15 % activity.
25359676	8	40	theme	scanning	1201:1208	arg1	SEM					1231:1233	SEM	1231:1233	SEM	1231:1233	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	8	40	theme	scanning	1201:1208	arg1	microscope					1219:1228	scanning electron microscope	1201:1228	scanning electron microscope (SEM)	1201:1234	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	2	41	theme	glutaraldehyde	414:427	arg1	v/v					450:452	1-4 % v/v	444:452	1-4 % v/v	444:452	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	2	41	theme	glutaraldehyde	414:427	arg1	concentration					429:441	glutaraldehyde concentration	414:441	glutaraldehyde concentration (1-4 % v/v)	414:453	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	0	42	theme	composite	48:56	arg1	beads					58:62	chitosan/TiO2 composite beads	34:62	chitosan/TiO2 composite beads	34:62	Synthesis and characterization of chitosan/TiO2 composite beads for improving stability of porcine pancreatic lipase.
25359676	3	43	theme	system	675:680	arg1	characterization					636:651	characterization	636:651	characterization of immobilized lipase system	636:680	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	1	44	from	immobilization	254:267	arg1	beads					296:300	chitosan/TiO2 composite beads	272:300	chitosan/TiO2 composite beads	272:300	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	1	45	theme	triacylglycerol	214:228	arg1	E.C.3.1.1.3					238:248	E.C.3.1.1.3	238:248	E.C.3.1.1.3	238:248	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	1	45	theme	triacylglycerol	214:228	arg1	lipase					230:235	triacylglycerol lipase	214:235	triacylglycerol lipase	214:235	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	0	46	theme	chitosan/TiO2	34:46	arg1	beads					58:62	chitosan/TiO2 composite beads	34:62	chitosan/TiO2 composite beads	34:62	Synthesis and characterization of chitosan/TiO2 composite beads for improving stability of porcine pancreatic lipase.
25359676	6	47	theme	free	861:864	arg1	lipase					866:871	free lipase	861:871	free lipase	861:871	The optimum pH values for the free lipase and immobilized lipase were found to be 6.5 and 7.5, respectively.
25359676	8	48	theme	attenuated	1302:1311	arg1	Fourier					1330:1336	attenuated total reflection Fourier	1302:1336	attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR)	1302:1388	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	9	49	theme	usage	1488:1492	arg1	production					1505:1514	the industrial usage especially production	1473:1514	the industrial usage especially production of biodiesel and dairy products	1473:1546	The significance of this study is improving of stability properties of lipase for the industrial usage especially production of biodiesel and dairy products.
25359676	8	50	theme	beads	1177:1181	arg1	characterization					1133:1148	the structural characterization	1118:1148	the structural characterization of chitosan/TiO2 composite beads	1118:1181	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	7	51	theme	soluble	1067:1073	arg1	lipase					1075:1080	soluble lipase	1067:1080	soluble lipase protected only 15 % activity	1067:1109	The thermal stability of immobilized lipase was evaluated, and it maintained 45 % activity at 70 °C. But, at this temperature, soluble lipase protected only 15 % activity.
25359676	8	52	theme	X-ray	1237:1241	arg1	XRD					1256:1258	XRD	1256:1258	XRD	1256:1258	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	8	52	theme	X-ray	1237:1241	arg1	diffraction					1243:1253	X-ray diffraction	1237:1253	X-ray diffraction (XRD)	1237:1259	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	8	53	dep	Fourier	1330:1336	arg1	transform					1338:1346	transform	1338:1346	transform infrared spectroscopy analysis (ATR-FTIR)	1338:1388	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	8	54	theme	thermal	1262:1268	arg1	analysis					1282:1289	thermal gravimetric analysis	1262:1289	thermal gravimetric analysis (TGA)	1262:1295	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	8	54	theme	thermal	1262:1268	arg1	TGA					1292:1294	TGA	1292:1294	TGA	1292:1294	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	8	55	theme	composite	1167:1175	arg1	beads					1177:1181	chitosan/TiO2 composite beads	1153:1181	chitosan/TiO2 composite beads	1153:1181	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	0	56	theme	beads	58:62	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of chitosan/TiO2 composite beads for improving stability of porcine pancreatic lipase.
25359676	0	56	theme	beads	58:62	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of chitosan/TiO2 composite beads for improving stability of porcine pancreatic lipase.
25359676	7	57	theme	thermal	944:950	arg1	stability					952:960	The thermal stability	940:960	The thermal stability of immobilized lipase	940:982	The thermal stability of immobilized lipase was evaluated, and it maintained 45 % activity at 70 °C. But, at this temperature, soluble lipase protected only 15 % activity.
25359676	8	58	theme	reflection	1319:1328	arg1	Fourier					1330:1336	attenuated total reflection Fourier	1302:1336	attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR)	1302:1388	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	6	59	dep	lipase	866:871	arg1	the					857:859	the	857:859	the	857:859	The optimum pH values for the free lipase and immobilized lipase were found to be 6.5 and 7.5, respectively.
25359676	2	60	theme	0.5-2	368:372	arg1	concentration					353:365	initial enzyme concentration	338:365	initial enzyme concentration (0.5-2 mg/ml)	338:379	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	2	60	theme	0.5-2	368:372	arg1	mg/ml					374:378	0.5-2 mg/ml	368:378	0.5-2 mg/ml	368:378	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	9	61	theme	lipase	1462:1467	arg1	properties					1448:1457	stability properties	1438:1457	stability properties of lipase	1438:1467	The significance of this study is improving of stability properties of lipase for the industrial usage especially production of biodiesel and dairy products.
25359676	4	62	theme	immobilized	720:730	arg1	lipase					732:737	free and immobilized lipase	711:737	free and immobilized lipase	711:737	The optimum temperatures of free and immobilized lipase were 30 °C.
25359676	3	63	theme	pH	564:565	arg1	4.0-9.0					578:584	4.0-9.0	578:584	4.0-9.0	578:584	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	3	63	theme	pH	564:565	arg1	stability					567:575	pH stability	564:575	pH stability (4.0-9.0)	564:585	The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system.
25359676	8	64	theme	gravimetric	1270:1280	arg1	analysis					1282:1289	thermal gravimetric analysis	1262:1289	thermal gravimetric analysis (TGA)	1262:1295	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	8	64	theme	gravimetric	1270:1280	arg1	TGA					1292:1294	TGA	1292:1294	TGA	1292:1294	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	1	65	theme	present	137:143	arg1	work					145:148	the present work	133:148	the present work	133:148	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	8	66	theme	electron	1210:1217	arg1	SEM					1231:1233	SEM	1231:1233	SEM	1231:1233	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	8	66	theme	electron	1210:1217	arg1	microscope					1219:1228	scanning electron microscope	1201:1228	scanning electron microscope (SEM)	1201:1234	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	1	67	dep	lipase	206:211	arg1	E.C.3.1.1.3					238:248	E.C.3.1.1.3	238:248	E.C.3.1.1.3	238:248	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	1	67	dep	lipase	206:211	arg1	lipase					230:235	triacylglycerol lipase	214:235	triacylglycerol lipase	214:235	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	5	68	theme	temperature	755:765	arg1	profile					767:773	The temperature profile	751:773	The temperature profile of the immobilized lipase	751:799	The temperature profile of the immobilized lipase was spread over a large area.
25359676	1	69	theme	work	145:148	arg1	purpose					122:128	The purpose	118:128	The purpose of the present work	118:148	The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads.
25359676	6	70	theme	pH	843:844	arg1	values					846:851	The optimum pH values	831:851	The optimum pH values for the free lipase and immobilized lipase	831:894	The optimum pH values for the free lipase and immobilized lipase were found to be 6.5 and 7.5, respectively.
25359676	6	70	theme	pH	843:844	arg1	6.5					913:915	6.5	913:915	6.5	913:915	The optimum pH values for the free lipase and immobilized lipase were found to be 6.5 and 7.5, respectively.
25359676	4	71	theme	lipase	732:737	arg1	temperatures					695:706	The optimum temperatures	683:706	The optimum temperatures of free and immobilized lipase	683:737	The optimum temperatures of free and immobilized lipase were 30 °C.
25359676	4	71	theme	lipase	732:737	arg1	°C					747:748	30 °C	744:748	30 °C	744:748	The optimum temperatures of free and immobilized lipase were 30 °C.
25359676	8	72	theme	structural	1122:1131	arg1	characterization					1133:1148	the structural characterization	1118:1148	the structural characterization of chitosan/TiO2 composite beads	1118:1181	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	2	73	theme	adsorption	382:391	arg1	min					404:406	5-25 min	399:406	5-25 min	399:406	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	2	73	theme	adsorption	382:391	arg1	time					393:396	adsorption time	382:396	adsorption time (5-25 min)	382:407	The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v).
25359676	8	74	theme	chitosan/TiO2	1153:1165	arg1	beads					1177:1181	chitosan/TiO2 composite beads	1153:1181	chitosan/TiO2 composite beads	1153:1181	Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR).
25359676	7	75	theme	lipase	1075:1080	arg1	activity					1102:1109	soluble lipase protected only 15 % activity	1067:1109	soluble lipase protected only 15 % activity	1067:1109	The thermal stability of immobilized lipase was evaluated, and it maintained 45 % activity at 70 °C. But, at this temperature, soluble lipase protected only 15 % activity.
24933885	5	0	theme	wall	621:624	arg1	components					626:635	the cell wall components	612:635	the cell wall components of trees exposed to increasing Cd amounts in soil	612:685	Another objective of this study was to compare the cell wall components of trees exposed to increasing Cd amounts in soil.
24933885	7	1	theme	cadmium	970:976	arg1	contamination					978:990	cadmium contamination	970:990	cadmium contamination	970:990	A concurrent reduction in methyl-esterification of pectin suggests than the structure of this major binding site could therefore be modified as a reaction to cadmium contamination.
24933885	0	2	theme	exposure	94:101	arg1	effects					75:81	effects	75:81	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.	0:127	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.
24933885	7	3	theme	concurrent	814:823	arg1	reduction					825:833	A concurrent reduction	812:833	A concurrent reduction in methyl-esterification of pectin	812:868	A concurrent reduction in methyl-esterification of pectin suggests than the structure of this major binding site could therefore be modified as a reaction to cadmium contamination.
24933885	0	4	theme	cadmium	86:92	arg1	exposure					94:101	cadmium exposure	86:101	cadmium exposure	86:101	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.
24933885	6	5	from	increase	716:723	arg1	lignin					702:707	lignin	702:707	lignin	702:707	A decrease in lignin and an increase in pectin contents were observed in response to increasing soil cadmium concentration.
24933885	6	5	from	increase	716:723	arg1	contents					735:742	pectin contents	728:742	pectin contents	728:742	A decrease in lignin and an increase in pectin contents were observed in response to increasing soil cadmium concentration.
24933885	6	6	from	decrease	690:697	arg1	lignin					702:707	lignin	702:707	lignin	702:707	A decrease in lignin and an increase in pectin contents were observed in response to increasing soil cadmium concentration.
24933885	6	6	from	decrease	690:697	arg1	contents					735:742	pectin contents	728:742	pectin contents	728:742	A decrease in lignin and an increase in pectin contents were observed in response to increasing soil cadmium concentration.
24933885	0	7	theme	cell	106:109	arg1	composition					116:126	cell wall composition	106:126	cell wall composition	106:126	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.
24933885	6	8	theme	pectin	728:733	arg1	contents					735:742	pectin contents	728:742	pectin contents	728:742	A decrease in lignin and an increase in pectin contents were observed in response to increasing soil cadmium concentration.
24933885	2	9	theme	mg/kg	323:327	arg1	DM					329:330	25 mg/kg DM	320:330	25 mg/kg DM	320:330	Most of the absorbed Cd is retained in roots (25 mg/kg DM), but transfer to aerial part is also effective.
24933885	2	9	theme	mg/kg	323:327	arg1	roots					313:317	roots	313:317	roots (25 mg/kg DM)	313:331	Most of the absorbed Cd is retained in roots (25 mg/kg DM), but transfer to aerial part is also effective.
24933885	7	10	from	reduction	825:833	arg1	methyl-esterification					838:858	methyl-esterification	838:858	methyl-esterification of pectin	838:868	A concurrent reduction in methyl-esterification of pectin suggests than the structure of this major binding site could therefore be modified as a reaction to cadmium contamination.
24933885	8	11	theme	Cd	1076:1077	arg1	exposure					1079:1086	Cd exposure	1076:1086	Cd exposure	1076:1086	Future prospects will focus on the modulation of pectin composition in response to Cd exposure.
24933885	5	12	theme	increasing	657:666	arg1	amounts					671:677	increasing Cd amounts	657:677	increasing Cd amounts	657:677	Another objective of this study was to compare the cell wall components of trees exposed to increasing Cd amounts in soil.
24933885	1	13	theme	Cd-contaminated	172:186	arg1	soil					188:191	an artificially Cd-contaminated soil	156:191	an artificially Cd-contaminated soil	156:191	Douglas fir trees grown on an artificially Cd-contaminated soil, can tolerate this trace element (up to 68 mg/kg in soil) during several months.
24933885	5	14	theme	Cd	668:669	arg1	amounts					671:677	increasing Cd amounts	657:677	increasing Cd amounts	657:677	Another objective of this study was to compare the cell wall components of trees exposed to increasing Cd amounts in soil.
24933885	3	15	dep	6	416:416	arg1	to					413:414	to	413:414	to	413:414	Showing the highest content, up to 6 mg/kg DM, among all the aboveground parts, barks seem to be a preferred storage compartment.
24933885	0	16	theme	soils	41:45	arg1	Phytoremediation					0:15	Phytoremediation	0:15	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.	0:127	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.
24933885	1	17	from	mg/kg	236:240	arg1	soil					245:248	soil	245:248	soil	245:248	Douglas fir trees grown on an artificially Cd-contaminated soil, can tolerate this trace element (up to 68 mg/kg in soil) during several months.
24933885	3	18	theme	aboveground	442:452	arg1	parts					454:458	all the aboveground parts	434:458	all the aboveground parts	434:458	Showing the highest content, up to 6 mg/kg DM, among all the aboveground parts, barks seem to be a preferred storage compartment.
24933885	2	19	theme	aerial	350:355	arg1	part					357:360	aerial part	350:360	aerial part	350:360	Most of the absorbed Cd is retained in roots (25 mg/kg DM), but transfer to aerial part is also effective.
24933885	0	20	theme	wall	111:114	arg1	composition					116:126	cell wall composition	106:126	cell wall composition	106:126	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.
24933885	6	21	dep	increasing	773:782	arg1	response					761:768	response	761:768	response	761:768	A decrease in lignin and an increase in pectin contents were observed in response to increasing soil cadmium concentration.
24933885	3	22	dep	DM	424:425	arg1	mg/kg					418:422	mg/kg	418:422	mg/kg	418:422	Showing the highest content, up to 6 mg/kg DM, among all the aboveground parts, barks seem to be a preferred storage compartment.
24933885	0	23	theme	young	50:54	arg1	trees					68:72	young Douglas fir trees	50:72	young Douglas fir trees	50:72	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.
24933885	1	24	theme	several	258:264	arg1	months					266:271	several months	258:271	several months	258:271	Douglas fir trees grown on an artificially Cd-contaminated soil, can tolerate this trace element (up to 68 mg/kg in soil) during several months.
24933885	7	25	theme	binding	912:918	arg1	site					920:923	this major binding site	901:923	this major binding site	901:923	A concurrent reduction in methyl-esterification of pectin suggests than the structure of this major binding site could therefore be modified as a reaction to cadmium contamination.
24933885	0	26	from	effects	75:81	arg1	composition					116:126	cell wall composition	106:126	cell wall composition	106:126	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.
24933885	8	27	theme	composition	1049:1059	arg1	modulation					1028:1037	the modulation	1024:1037	the modulation of pectin composition in response to Cd exposure	1024:1086	Future prospects will focus on the modulation of pectin composition in response to Cd exposure.
24933885	4	28	theme	transfer	524:531	arg1	factor					533:538	the transfer factor	520:538	the transfer factor	520:538	However, the transfer factor is quite low, about 0.3.
24933885	4	28	theme	transfer	524:531	arg1	low					549:551	low	549:551	low	549:551	However, the transfer factor is quite low, about 0.3.
24933885	0	29	theme	cadmium-contaminated	20:39	arg1	soils					41:45	cadmium-contaminated soils	20:45	cadmium-contaminated soils	20:45	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.
24933885	6	30	theme	cadmium	789:795	arg1	concentration					797:809	soil cadmium concentration	784:809	soil cadmium concentration	784:809	A decrease in lignin and an increase in pectin contents were observed in response to increasing soil cadmium concentration.
24933885	5	31	theme	trees	640:644	arg1	components					626:635	the cell wall components	612:635	the cell wall components of trees exposed to increasing Cd amounts in soil	612:685	Another objective of this study was to compare the cell wall components of trees exposed to increasing Cd amounts in soil.
24933885	0	32	theme	fir	64:66	arg1	trees					68:72	young Douglas fir trees	50:72	young Douglas fir trees	50:72	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.
24933885	5	33	theme	study	591:595	arg1	objective					573:581	Another objective	565:581	Another objective of this study	565:595	Another objective of this study was to compare the cell wall components of trees exposed to increasing Cd amounts in soil.
24933885	7	34	theme	site	920:923	arg1	structure					888:896	the structure	884:896	the structure of this major binding site	884:923	A concurrent reduction in methyl-esterification of pectin suggests than the structure of this major binding site could therefore be modified as a reaction to cadmium contamination.
24933885	7	34	theme	site	920:923	arg1	reaction					958:965	a reaction	956:965	a reaction to cadmium contamination	956:990	A concurrent reduction in methyl-esterification of pectin suggests than the structure of this major binding site could therefore be modified as a reaction to cadmium contamination.
24933885	0	35	theme	Douglas	56:62	arg1	trees					68:72	young Douglas fir trees	50:72	young Douglas fir trees	50:72	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.
24933885	7	36	theme	major	906:910	arg1	site					920:923	this major binding site	901:923	this major binding site	901:923	A concurrent reduction in methyl-esterification of pectin suggests than the structure of this major binding site could therefore be modified as a reaction to cadmium contamination.
24933885	8	37	from	modulation	1028:1037	arg1	response					1064:1071	response	1064:1071	response to Cd exposure	1064:1086	Future prospects will focus on the modulation of pectin composition in response to Cd exposure.
24933885	7	38	theme	pectin	863:868	arg1	methyl-esterification					838:858	methyl-esterification	838:858	methyl-esterification of pectin	838:868	A concurrent reduction in methyl-esterification of pectin suggests than the structure of this major binding site could therefore be modified as a reaction to cadmium contamination.
24933885	3	39	theme	storage	490:496	arg1	compartment					498:508	a preferred storage compartment	478:508	a preferred storage compartment	478:508	Showing the highest content, up to 6 mg/kg DM, among all the aboveground parts, barks seem to be a preferred storage compartment.
24933885	1	40	dep	tolerate	198:205	arg1	mg/kg					236:240	up to 68 mg/kg	227:240	up to 68 mg/kg in soil	227:248	Douglas fir trees grown on an artificially Cd-contaminated soil, can tolerate this trace element (up to 68 mg/kg in soil) during several months.
24933885	2	41	theme	absorbed	286:293	arg1	Cd					295:296	the absorbed Cd	282:296	the absorbed Cd	282:296	Most of the absorbed Cd is retained in roots (25 mg/kg DM), but transfer to aerial part is also effective.
24933885	3	42	dep	mg/kg	418:422	arg1	6					416:416	6	416:416	6	416:416	Showing the highest content, up to 6 mg/kg DM, among all the aboveground parts, barks seem to be a preferred storage compartment.
24933885	1	43	theme	trace	212:216	arg1	element					218:224	this trace element	207:224	this trace element	207:224	Douglas fir trees grown on an artificially Cd-contaminated soil, can tolerate this trace element (up to 68 mg/kg in soil) during several months.
24933885	1	44	theme	Douglas	129:135	arg1	trees					141:145	Douglas fir trees	129:145	Douglas fir trees	129:145	Douglas fir trees grown on an artificially Cd-contaminated soil, can tolerate this trace element (up to 68 mg/kg in soil) during several months.
24933885	3	45	theme	highest	393:399	arg1	content					401:407	the highest content	389:407	the highest content	389:407	Showing the highest content, up to 6 mg/kg DM, among all the aboveground parts, barks seem to be a preferred storage compartment.
24933885	8	46	theme	Future	993:998	arg1	prospects					1000:1008	Future prospects	993:1008	Future prospects	993:1008	Future prospects will focus on the modulation of pectin composition in response to Cd exposure.
24933885	1	47	dep	68	233:234	arg1	to					230:231	to	230:231	to	230:231	Douglas fir trees grown on an artificially Cd-contaminated soil, can tolerate this trace element (up to 68 mg/kg in soil) during several months.
24933885	3	48	theme	preferred	480:488	arg1	compartment					498:508	a preferred storage compartment	478:508	a preferred storage compartment	478:508	Showing the highest content, up to 6 mg/kg DM, among all the aboveground parts, barks seem to be a preferred storage compartment.
24933885	1	49	theme	fir	137:139	arg1	trees					141:145	Douglas fir trees	129:145	Douglas fir trees	129:145	Douglas fir trees grown on an artificially Cd-contaminated soil, can tolerate this trace element (up to 68 mg/kg in soil) during several months.
24933885	5	50	theme	cell	616:619	arg1	components					626:635	the cell wall components	612:635	the cell wall components of trees exposed to increasing Cd amounts in soil	612:685	Another objective of this study was to compare the cell wall components of trees exposed to increasing Cd amounts in soil.
24933885	0	51	dep	Phytoremediation	0:15	arg1	effects					75:81	effects	75:81	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.	0:127	Phytoremediation of cadmium-contaminated soils by young Douglas fir trees: effects of cadmium exposure on cell wall composition.
24933885	8	52	theme	pectin	1042:1047	arg1	composition					1049:1059	pectin composition	1042:1059	pectin composition	1042:1059	Future prospects will focus on the modulation of pectin composition in response to Cd exposure.
24933885	6	53	theme	soil	784:787	arg1	concentration					797:809	soil cadmium concentration	784:809	soil cadmium concentration	784:809	A decrease in lignin and an increase in pectin contents were observed in response to increasing soil cadmium concentration.
27287146	0	0	with	complexation	30:41	arg1	chitosan					102:109	chitosan	102:109	chitosan	102:109	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
27287146	2	1	theme	novel	314:318	arg1	type					320:323	a novel type	312:323	a novel type of magnetically responsive chitosan-based composite material	312:384	This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material.
27287146	6	2	dep	cells	902:906	arg1	cerevisiae					923:932	Saccharomyces cerevisiae	909:932	Saccharomyces cerevisiae	909:932	Baker's yeast cells (Saccharomyces cerevisiae) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates.
27287146	5	3	theme	chitosan	857:864	arg1	activation					843:852	activation	843:852	activation	843:852	Lipase from Candida rugosa was covalently attached after cross-linking and activation of chitosan using glutaraldehyde.
27287146	5	3	theme	chitosan	857:864	arg1	cross-linking					825:837	cross-linking	825:837	cross-linking	825:837	Lipase from Candida rugosa was covalently attached after cross-linking and activation of chitosan using glutaraldehyde.
27287146	6	4	theme	chitosan	962:969	arg1	composite					971:979	the chitosan composite	958:979	the chitosan composite	958:979	Baker's yeast cells (Saccharomyces cerevisiae) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates.
27287146	0	5	theme	magnetic	82:89	arg1	fluid					91:95	stabilized magnetic fluid	71:95	stabilized magnetic fluid	71:95	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
27287146	4	6	theme	magnetic	710:717	arg1	carrier					719:725	a magnetic carrier	708:725	a magnetic carrier for immobilization of enzymes and cells	708:765	Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells.
27287146	4	7	theme	Magnetic	608:615	arg1	material					656:663	a perspective material	642:663	a perspective material for various bioapplications	642:691	Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells.
27287146	4	7	theme	Magnetic	608:615	arg1	chitosan					617:624	Magnetic chitosan	608:624	Magnetic chitosan	608:624	Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells.
27287146	0	8	theme	stabilized	71:80	arg1	fluid					91:95	stabilized magnetic fluid	71:95	stabilized magnetic fluid	71:95	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
27287146	3	9	theme	optical	486:492	arg1	microscopy					494:503	optical microscopy	486:503	optical microscopy	486:503	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	3	9	theme	optical	486:492	arg1	SEM					511:513	SEM	511:513	SEM	511:513	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	3	9	theme	optical	486:492	arg1	TEM					506:508	TEM	506:508	TEM	506:508	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	1	10	theme	methacrylic	238:248	arg1	poly					233:236	poly	233:236	poly(methacrylic acid)	233:254	A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed.
27287146	1	10	theme	methacrylic	238:248	arg1	acid					250:253	methacrylic acid	238:253	methacrylic acid	238:253	A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed.
27287146	6	11	theme	appropriate	1055:1065	arg1	substrates					1067:1076	appropriate substrates	1055:1076	appropriate substrates	1055:1076	Baker's yeast cells (Saccharomyces cerevisiae) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates.
27287146	3	12	dep	microscopy	474:483	arg1	microscopy					494:503	optical microscopy	486:503	optical microscopy	486:503	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	3	12	dep	microscopy	474:483	arg1	SEM					511:513	SEM	511:513	SEM	511:513	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	3	12	dep	microscopy	474:483	arg1	TEM					506:508	TEM	506:508	TEM	506:508	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	2	13	theme	composite	367:375	arg1	material					377:384	magnetically responsive chitosan-based composite material	328:384	magnetically responsive chitosan-based composite material	328:384	This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material.
27287146	0	14	theme	fluid	91:95	arg1	complexation					30:41	complexation	30:41	complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan	30:109	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
27287146	2	15	theme	chitosan-based	352:365	arg1	material					377:384	magnetically responsive chitosan-based composite material	328:384	magnetically responsive chitosan-based composite material	328:384	This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material.
27287146	3	16	dep	static	529:534	arg1	SANS					566:569	SANS	566:569	SANS	566:569	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	2	17	used	used	296:299	arg2	ferrofluid					281:290	This ferrofluid	276:290	This ferrofluid	276:290	This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material.
27287146	2	18	theme	responsive	341:350	arg1	material					377:384	magnetically responsive chitosan-based composite material	328:384	magnetically responsive chitosan-based composite material	328:384	This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material.
27287146	0	19	theme	Composite	0:8	arg1	particles					10:18	Composite particles	0:18	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan	0:109	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
27287146	3	20	theme	magnetic	407:414	arg1	composite					425:433	magnetic chitosan composite	407:433	magnetic chitosan composite	407:433	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	3	21	theme	scattering	517:526	arg1	techniques					596:605	microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques	474:605	microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques	474:605	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	5	22	theme	Candida	780:786	arg1	rugosa					788:793	Candida rugosa	780:793	Candida rugosa	780:793	Lipase from Candida rugosa was covalently attached after cross-linking and activation of chitosan using glutaraldehyde.
27287146	6	23	with	reaction	1041:1048	arg1	substrates					1067:1076	appropriate substrates	1055:1076	appropriate substrates	1055:1076	Baker's yeast cells (Saccharomyces cerevisiae) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates.
27287146	3	24	theme	techniques	596:605	arg1	combination					459:469	a combination	457:469	a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques	457:605	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	1	25	theme	magnetic	191:198	arg1	ferrofluid					207:216	ferrofluid	207:216	ferrofluid	207:216	A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed.
27287146	1	25	theme	magnetic	191:198	arg1	fluid					200:204	magnetic fluid	191:204	magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid)	191:254	A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed.
27287146	1	26	theme	simple	153:158	arg1	procedure					160:168	A simple procedure	151:168	A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid)	151:254	A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed.
27287146	0	27	theme	Magnetic	112:119	arg1	material					121:128	Magnetic material	112:128	Magnetic material for bioapplications	112:148	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
27287146	4	28	theme	cells	761:765	arg1	immobilization					731:744	immobilization	731:744	immobilization of enzymes and cells	731:765	Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells.
27287146	1	29	theme	fluid	200:204	arg1	synthesis					178:186	the synthesis	174:186	the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid)	174:254	A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed.
27287146	3	30	theme	chitosan	416:423	arg1	composite					425:433	magnetic chitosan composite	407:433	magnetic chitosan composite	407:433	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	3	31	theme	dynamic	540:546	arg1	scattering					554:563	dynamic light scattering	540:563	dynamic light scattering	540:563	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	3	31	theme	dynamic	540:546	arg1	scattering					517:526	scattering	517:526	scattering (static and dynamic light scattering, SANS)	517:570	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	3	32	theme	microscopy	474:483	arg1	techniques					596:605	microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques	474:605	microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques	474:605	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	0	33	theme	poly	46:49	arg1	complexation					30:41	complexation	30:41	complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan	30:109	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
27287146	3	34	theme	light	548:552	arg1	scattering					554:563	dynamic light scattering	540:563	dynamic light scattering	540:563	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	3	34	theme	light	548:552	arg1	scattering					517:526	scattering	517:526	scattering (static and dynamic light scattering, SANS)	517:570	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	4	35	theme	perspective	644:654	arg1	material					656:663	a perspective material	642:663	a perspective material for various bioapplications	642:691	Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells.
27287146	4	35	theme	perspective	644:654	arg1	chitosan					617:624	Magnetic chitosan	608:624	Magnetic chitosan	608:624	Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells.
27287146	6	36	theme	yeast	896:900	arg1	cells					902:906	Baker's yeast cells	888:906	Baker's yeast cells (Saccharomyces cerevisiae)	888:933	Baker's yeast cells (Saccharomyces cerevisiae) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates.
27287146	5	37	from	rugosa	788:793	arg1	Lipase					768:773	Lipase	768:773	Lipase from Candida rugosa	768:793	Lipase from Candida rugosa was covalently attached after cross-linking and activation of chitosan using glutaraldehyde.
27287146	4	38	theme	various	669:675	arg1	bioapplications					677:691	various bioapplications	669:691	various bioapplications	669:691	Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells.
27287146	3	39	theme	spectroscopy	576:587	arg1	techniques					596:605	microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques	474:605	microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques	474:605	Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques.
27287146	2	40	theme	material	377:384	arg1	type					320:323	a novel type	312:323	a novel type of magnetically responsive chitosan-based composite material	312:384	This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material.
27287146	0	41	dep	material	121:128	arg1	particles					10:18	Composite particles	0:18	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan	0:109	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
27287146	0	42	theme	methacrylic	51:61	arg1	poly					46:49	poly	46:49	poly(methacrylic acid)	46:67	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
27287146	0	42	theme	methacrylic	51:61	arg1	acid					63:66	methacrylic acid	51:66	methacrylic acid	51:66	Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
27287146	4	43	theme	enzymes	749:755	arg1	immobilization					731:744	immobilization	731:744	immobilization of enzymes and cells	731:765	Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells.
26309153	0	0	theme	Purified	68:75	arg1	Enzymes					77:83	Purified Enzymes	68:83	Purified Enzymes	68:83	Profiling the Hydrolysis of Isolated Grape Berry Skin Cell Walls by Purified Enzymes.
26309153	3	1	theme	grape	641:645	arg1	polymers					657:664	the grape cell wall polymers	637:664	the grape cell wall polymers due to enzyme activity	637:687	By using cell wall profiling techniques we could monitor the compositional changes in the grape cell wall polymers due to enzyme activity.
26309153	3	2	theme	enzyme	673:678	arg1	activity					680:687	enzyme activity	673:687	enzyme activity	673:687	By using cell wall profiling techniques we could monitor the compositional changes in the grape cell wall polymers due to enzyme activity.
26309153	4	3	theme	polymers	996:1003	arg1	extraction					982:991	extraction	982:991	extraction of polymers	982:1003	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	2	4	theme	buffered	430:437	arg1	conditions					439:448	buffered conditions	430:448	buffered conditions	430:448	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	2	5	theme	cell	539:542	arg1	walls					544:548	the grape skin cell walls	524:548	the grape skin cell walls	524:548	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	2	6	theme	skin	534:537	arg1	walls					544:548	the grape skin cell walls	524:548	the grape skin cell walls	524:548	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	4	7	theme	enzymatic	700:708	arg1	hydrolysis					710:719	Extensive enzymatic hydrolysis	690:719	Extensive enzymatic hydrolysis	690:719	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	5	8	theme	specific	1108:1115	arg1	associations					1135:1146	specific cell wall polymer associations	1108:1146	specific cell wall polymer associations	1108:1146	Synergistic enzyme activity was detectable as well as indications of specific cell wall polymer associations.
26309153	5	9	theme	Synergistic	1039:1049	arg1	activity					1058:1065	Synergistic enzyme activity	1039:1065	Synergistic enzyme activity	1039:1065	Synergistic enzyme activity was detectable as well as indications of specific cell wall polymer associations.
26309153	2	10	theme	grape	528:532	arg1	walls					544:548	the grape skin cell walls	524:548	the grape skin cell walls	524:548	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	2	11	dep	substrate	320:328	arg1	using					334:338	using	334:338	using isolated grape skin cell walls	334:369	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	1	12	theme	substrate	244:252	arg1	nature					225:230	the complex and rather undefined nature	192:230	the complex and rather undefined nature of both the substrate and the enzyme preparations	192:280	The unraveling of crushed grapes by maceration enzymes during winemaking is difficult to study because of the complex and rather undefined nature of both the substrate and the enzyme preparations.
26309153	4	13	theme	extensive	910:918	arg1	hydrolysis					920:929	less extensive hydrolysis	905:929	less extensive hydrolysis	905:929	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	3	14	theme	due	666:668	arg1	polymers					657:664	the grape cell wall polymers	637:664	the grape cell wall polymers due to enzyme activity	637:687	By using cell wall profiling techniques we could monitor the compositional changes in the grape cell wall polymers due to enzyme activity.
26309153	2	15	theme	cell	360:363	arg1	walls					365:369	isolated grape skin cell walls	340:369	isolated grape skin cell walls	340:369	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	4	16	theme	wall	955:958	arg1	structure					960:968	the cell wall structure	946:968	the cell wall structure	946:968	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	3	17	theme	cell	647:650	arg1	polymers					657:664	the grape cell wall polymers	637:664	the grape cell wall polymers due to enzyme activity	637:687	By using cell wall profiling techniques we could monitor the compositional changes in the grape cell wall polymers due to enzyme activity.
26309153	3	18	from	changes	626:632	arg1	polymers					657:664	the grape cell wall polymers	637:664	the grape cell wall polymers due to enzyme activity	637:687	By using cell wall profiling techniques we could monitor the compositional changes in the grape cell wall polymers due to enzyme activity.
26309153	2	19	theme	skin	355:358	arg1	walls					365:369	isolated grape skin cell walls	340:369	isolated grape skin cell walls	340:369	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	3	20	theme	wall	652:655	arg1	polymers					657:664	the grape cell wall polymers	637:664	the grape cell wall polymers due to enzyme activity	637:687	By using cell wall profiling techniques we could monitor the compositional changes in the grape cell wall polymers due to enzyme activity.
26309153	5	21	theme	associations	1135:1146	arg1	indications					1093:1103	indications	1093:1103	indications of specific cell wall polymer associations	1093:1146	Synergistic enzyme activity was detectable as well as indications of specific cell wall polymer associations.
26309153	5	22	theme	enzyme	1051:1056	arg1	activity					1058:1065	Synergistic enzyme activity	1039:1065	Synergistic enzyme activity	1039:1065	Synergistic enzyme activity was detectable as well as indications of specific cell wall polymer associations.
26309153	2	23	theme	grape	349:353	arg1	walls					365:369	isolated grape skin cell walls	340:369	isolated grape skin cell walls	340:369	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	5	24	theme	wall	1122:1125	arg1	associations					1135:1146	specific cell wall polymer associations	1108:1146	specific cell wall polymer associations	1108:1146	Synergistic enzyme activity was detectable as well as indications of specific cell wall polymer associations.
26309153	4	25	theme	cellulase	792:800	arg1	enzymes					819:825	cellulase or hemicellulase enzymes	792:825	enzymes	819:825	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	2	26	theme	isolated	340:347	arg1	walls					365:369	isolated grape skin cell walls	340:369	isolated grape skin cell walls	340:369	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	5	27	theme	polymer	1127:1133	arg1	associations					1135:1146	specific cell wall polymer associations	1108:1146	specific cell wall polymer associations	1108:1146	Synergistic enzyme activity was detectable as well as indications of specific cell wall polymer associations.
26309153	4	28	theme	cell	950:953	arg1	structure					960:968	the cell wall structure	946:968	the cell wall structure	946:968	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	4	29	theme	hemicellulase	805:817	arg1	enzymes					819:825	cellulase or hemicellulase enzymes	792:825	enzymes	819:825	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	2	30	theme	enzyme	380:385	arg1	preparations					387:398	the enzyme preparations	376:398	the enzyme preparations	376:398	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	1	31	theme	enzyme	262:267	arg1	preparations					269:280	the enzyme preparations	258:280	the enzyme preparations	258:280	The unraveling of crushed grapes by maceration enzymes during winemaking is difficult to study because of the complex and rather undefined nature of both the substrate and the enzyme preparations.
26309153	2	32	theme	individual	489:498	arg1	enzymes					513:519	the individual and combined enzymes	485:519	enzymes	513:519	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	4	33	theme	Extensive	690:698	arg1	hydrolysis					710:719	Extensive enzymatic hydrolysis	690:719	Extensive enzymatic hydrolysis	690:719	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	2	34	theme	purified	410:417	arg1	enzymes					419:425	purified enzymes	410:425	purified enzymes	410:425	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	0	35	theme	Grape	37:41	arg1	Walls					59:63	Isolated Grape Berry Skin Cell Walls	28:63	Isolated Grape Berry Skin Cell Walls	28:63	Profiling the Hydrolysis of Isolated Grape Berry Skin Cell Walls by Purified Enzymes.
26309153	1	36	theme	preparations	269:280	arg1	nature					225:230	the complex and rather undefined nature	192:230	the complex and rather undefined nature of both the substrate and the enzyme preparations	192:280	The unraveling of crushed grapes by maceration enzymes during winemaking is difficult to study because of the complex and rather undefined nature of both the substrate and the enzyme preparations.
26309153	4	37	theme	polymers	887:894	arg1	levels					870:875	levels	870:875	levels of pectin polymers	870:894	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	4	38	theme	pectinases	753:762	arg1	preparation					738:748	a preparation	736:748	a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes	736:825	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	0	39	theme	Isolated	28:35	arg1	Walls					59:63	Isolated Grape Berry Skin Cell Walls	28:63	Isolated Grape Berry Skin Cell Walls	28:63	Profiling the Hydrolysis of Isolated Grape Berry Skin Cell Walls by Purified Enzymes.
26309153	4	40	theme	pectin	880:885	arg1	polymers					887:894	pectin polymers	880:894	pectin polymers	880:894	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	3	41	theme	wall	565:568	arg1	techniques					580:589	cell wall profiling techniques	560:589	cell wall profiling techniques	560:589	By using cell wall profiling techniques we could monitor the compositional changes in the grape cell wall polymers due to enzyme activity.
26309153	1	42	theme	complex	196:202	arg1	nature					225:230	the complex and rather undefined nature	192:230	the complex and rather undefined nature of both the substrate and the enzyme preparations	192:280	The unraveling of crushed grapes by maceration enzymes during winemaking is difficult to study because of the complex and rather undefined nature of both the substrate and the enzyme preparations.
26309153	0	43	theme	Skin	49:52	arg1	Walls					59:63	Isolated Grape Berry Skin Cell Walls	28:63	Isolated Grape Berry Skin Cell Walls	28:63	Profiling the Hydrolysis of Isolated Grape Berry Skin Cell Walls by Purified Enzymes.
26309153	3	44	theme	profiling	570:578	arg1	techniques					580:589	cell wall profiling techniques	560:589	cell wall profiling techniques	560:589	By using cell wall profiling techniques we could monitor the compositional changes in the grape cell wall polymers due to enzyme activity.
26309153	4	45	theme	pectinases	767:776	arg1	preparation					738:748	a preparation	736:748	a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes	736:825	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	0	46	theme	Berry	43:47	arg1	Walls					59:63	Isolated Grape Berry Skin Cell Walls	28:63	Isolated Grape Berry Skin Cell Walls	28:63	Profiling the Hydrolysis of Isolated Grape Berry Skin Cell Walls by Purified Enzymes.
26309153	4	47	dep	removed	839:845	arg1	whereas					897:903	whereas	897:903	whereas	897:903	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	1	48	theme	crushed	104:110	arg1	grapes					112:117	crushed grapes	104:117	crushed grapes	104:117	The unraveling of crushed grapes by maceration enzymes during winemaking is difficult to study because of the complex and rather undefined nature of both the substrate and the enzyme preparations.
26309153	4	49	theme	wall	1026:1029	arg1	layers					1031:1036	the cell wall layers	1017:1036	the cell wall layers	1017:1036	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	5	50	theme	cell	1117:1120	arg1	associations					1135:1146	specific cell wall polymer associations	1108:1146	specific cell wall polymer associations	1108:1146	Synergistic enzyme activity was detectable as well as indications of specific cell wall polymer associations.
26309153	2	51	theme	enzymes	513:519	arg1	impact					475:480	the impact	471:480	the impact of the individual and combined enzymes on the grape skin cell walls	471:548	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	3	52	theme	compositional	612:624	arg1	changes					626:632	the compositional changes	608:632	the compositional changes in the grape cell wall polymers due to enzyme activity	608:687	By using cell wall profiling techniques we could monitor the compositional changes in the grape cell wall polymers due to enzyme activity.
26309153	1	53	theme	grapes	112:117	arg1	difficult					162:170	difficult	162:170	difficult	162:170	The unraveling of crushed grapes by maceration enzymes during winemaking is difficult to study because of the complex and rather undefined nature of both the substrate and the enzyme preparations.
26309153	1	53	theme	grapes	112:117	arg1	unraveling					90:99	The unraveling	86:99	The unraveling of crushed grapes by maceration enzymes during winemaking	86:157	The unraveling of crushed grapes by maceration enzymes during winemaking is difficult to study because of the complex and rather undefined nature of both the substrate and the enzyme preparations.
26309153	4	54	theme	cell	1021:1024	arg1	layers					1031:1036	the cell wall layers	1017:1036	the cell wall layers	1017:1036	Extensive enzymatic hydrolysis, achieved with a preparation of pectinases or pectinases combined with cellulase or hemicellulase enzymes, completely removed or drastically reduced levels of pectin polymers, whereas less extensive hydrolysis only opened up the cell wall structure and allowed extraction of polymers from within the cell wall layers.
26309153	2	55	theme	combined	504:511	arg1	enzymes					513:519	the individual and combined enzymes	485:519	enzymes	513:519	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	2	56	from	impact	475:480	arg1	walls					544:548	the grape skin cell walls	524:548	the grape skin cell walls	524:548	In this study we simplified both the substrate, by using isolated grape skin cell walls, and the enzyme preparations, by using purified enzymes in buffered conditions, to carefully follow the impact of the individual and combined enzymes on the grape skin cell walls.
26309153	3	57	theme	cell	560:563	arg1	techniques					580:589	cell wall profiling techniques	560:589	cell wall profiling techniques	560:589	By using cell wall profiling techniques we could monitor the compositional changes in the grape cell wall polymers due to enzyme activity.
26309153	0	58	theme	Walls	59:63	arg1	Hydrolysis					14:23	the Hydrolysis	10:23	the Hydrolysis of Isolated Grape Berry Skin Cell Walls by Purified Enzymes	10:83	Profiling the Hydrolysis of Isolated Grape Berry Skin Cell Walls by Purified Enzymes.
26309153	1	59	theme	maceration	122:131	arg1	enzymes					133:139	maceration enzymes	122:139	maceration enzymes	122:139	The unraveling of crushed grapes by maceration enzymes during winemaking is difficult to study because of the complex and rather undefined nature of both the substrate and the enzyme preparations.
26309153	1	60	theme	undefined	215:223	arg1	nature					225:230	the complex and rather undefined nature	192:230	the complex and rather undefined nature of both the substrate and the enzyme preparations	192:280	The unraveling of crushed grapes by maceration enzymes during winemaking is difficult to study because of the complex and rather undefined nature of both the substrate and the enzyme preparations.
26309153	0	61	theme	Cell	54:57	arg1	Walls					59:63	Isolated Grape Berry Skin Cell Walls	28:63	Isolated Grape Berry Skin Cell Walls	28:63	Profiling the Hydrolysis of Isolated Grape Berry Skin Cell Walls by Purified Enzymes.
25023201	2	0	theme	bioavailable	460:471	arg1	P					473:473	less bioavailable P	455:473	less bioavailable P	455:473	In contrast, synthetic apatite nanoparticles could hypothetically supply sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae in comparison to the soluble counterparts.
25023201	4	1	theme	pure	814:817	arg1	composition					798:808	the chemical composition	785:808	the chemical composition	785:808	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	4	1	theme	pure	814:817	arg1	hydroxyapatite					819:832	pure hydroxyapatite	814:832	pure hydroxyapatite	814:832	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	4	2	from	shape	743:747	arg1	spherical					730:738	spherical	730:738	spherical	730:738	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	4	2	from	shape	743:747	arg1	particles					669:677	The particles	665:677	The particles	665:677	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	2	3	theme	apatite	322:328	arg1	nanoparticles					330:342	synthetic apatite nanoparticles	312:342	synthetic apatite nanoparticles	312:342	In contrast, synthetic apatite nanoparticles could hypothetically supply sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae in comparison to the soluble counterparts.
25023201	0	4	theme	Glycine	72:78	arg1	soybean					63:69	soybean	63:69	soybean (Glycine max)	63:83	Synthetic apatite nanoparticles as a phosphorus fertilizer for soybean (Glycine max).
25023201	0	4	theme	Glycine	72:78	arg1	max					80:82	Glycine max	72:82	Glycine max	72:82	Synthetic apatite nanoparticles as a phosphorus fertilizer for soybean (Glycine max).
25023201	2	5	theme	synthetic	312:320	arg1	nanoparticles					330:342	synthetic apatite nanoparticles	312:342	synthetic apatite nanoparticles	312:342	In contrast, synthetic apatite nanoparticles could hypothetically supply sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae in comparison to the soluble counterparts.
25023201	2	6	from	mobility	418:425	arg1	environment					434:444	the environment	430:444	the environment	430:444	In contrast, synthetic apatite nanoparticles could hypothetically supply sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae in comparison to the soluble counterparts.
25023201	4	7	from	spherical	730:738	arg1	shape					743:747	shape	743:747	shape	743:747	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	4	8	theme	chemical	708:715	arg1	method					717:722	one-step wet chemical method	695:722	one-step wet chemical method	695:722	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	1	9	theme	solid	234:238	arg1	phosphates					240:249	solid phosphates	234:249	solid phosphates	234:249	Some soluble phosphate salts, heavily used in agriculture as highly effective phosphorus (P) fertilizers, cause surface water eutrophication, while solid phosphates are less effective in supplying the nutrient P.
25023201	6	10	theme	Biomass	1042:1048	arg1	productions					1050:1060	Biomass productions	1042:1060	Biomass productions	1042:1060	Biomass productions were enhanced by 18.2% (above-ground) and 41.2% (below-ground).
25023201	5	11	theme	regular	1007:1013	arg1	fertilizer					1017:1026	a regular P fertilizer	1005:1026	a regular P fertilizer (Ca(H2PO4)2)	1005:1039	The data show that application of the nanoparticles increased the growth rate and seed yield by 32.6% and 20.4%, respectively, compared to those of soybeans treated with a regular P fertilizer (Ca(H2PO4)2).
25023201	5	11	theme	regular	1007:1013	arg1	H2PO4					1032:1036	Ca(H2PO4)2	1029:1038	Ca(H2PO4)2	1029:1038	The data show that application of the nanoparticles increased the growth rate and seed yield by 32.6% and 20.4%, respectively, compared to those of soybeans treated with a regular P fertilizer (Ca(H2PO4)2).
25023201	5	12	theme	seed	917:920	arg1	yield					922:926	seed yield	917:926	seed yield	917:926	The data show that application of the nanoparticles increased the growth rate and seed yield by 32.6% and 20.4%, respectively, compared to those of soybeans treated with a regular P fertilizer (Ca(H2PO4)2).
25023201	7	13	theme	apatite	1132:1138	arg1	class					1163:1167	a new class	1157:1167	a new class of P fertilizer	1157:1183	Using apatite nanoparticles as a new class of P fertilizer can potentially enhance agronomical yield and reduce risks of water eutrophication.
25023201	7	13	theme	apatite	1132:1138	arg1	nanoparticles					1140:1152	apatite nanoparticles	1132:1152	apatite nanoparticles	1132:1152	Using apatite nanoparticles as a new class of P fertilizer can potentially enhance agronomical yield and reduce risks of water eutrophication.
25023201	5	14	theme	P	1015:1015	arg1	fertilizer					1017:1026	a regular P fertilizer	1005:1026	a regular P fertilizer (Ca(H2PO4)2)	1005:1039	The data show that application of the nanoparticles increased the growth rate and seed yield by 32.6% and 20.4%, respectively, compared to those of soybeans treated with a regular P fertilizer (Ca(H2PO4)2).
25023201	5	14	theme	P	1015:1015	arg1	H2PO4					1032:1036	Ca(H2PO4)2	1029:1038	Ca(H2PO4)2	1029:1038	The data show that application of the nanoparticles increased the growth rate and seed yield by 32.6% and 20.4%, respectively, compared to those of soybeans treated with a regular P fertilizer (Ca(H2PO4)2).
25023201	7	15	theme	P	1172:1172	arg1	fertilizer					1174:1183	P fertilizer	1172:1183	P fertilizer	1172:1183	Using apatite nanoparticles as a new class of P fertilizer can potentially enhance agronomical yield and reduce risks of water eutrophication.
25023201	2	16	theme	P	383:383	arg1	nutrients					385:393	sufficient P nutrients	372:393	sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae	372:482	In contrast, synthetic apatite nanoparticles could hypothetically supply sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae in comparison to the soluble counterparts.
25023201	1	17	theme	effective	154:162	arg1	phosphorus					164:173	highly effective phosphorus	147:173	highly effective phosphorus (P) fertilizers	147:189	Some soluble phosphate salts, heavily used in agriculture as highly effective phosphorus (P) fertilizers, cause surface water eutrophication, while solid phosphates are less effective in supplying the nutrient P.
25023201	1	17	theme	effective	154:162	arg1	P					176:176	P	176:176	P	176:176	Some soluble phosphate salts, heavily used in agriculture as highly effective phosphorus (P) fertilizers, cause surface water eutrophication, while solid phosphates are less effective in supplying the nutrient P.
25023201	2	18	with	crops	398:402	arg1	mobility					418:425	less mobility	413:425	less mobility in the environment	413:444	In contrast, synthetic apatite nanoparticles could hypothetically supply sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae in comparison to the soluble counterparts.
25023201	2	18	with	crops	398:402	arg1	P					473:473	less bioavailable P	455:473	less bioavailable P	455:473	In contrast, synthetic apatite nanoparticles could hypothetically supply sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae in comparison to the soluble counterparts.
25023201	4	19	theme	wet	704:706	arg1	method					717:722	one-step wet chemical method	695:722	one-step wet chemical method	695:722	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	3	20	from	effect	597:602	arg1	soybean					642:648	soybean	642:648	soybean (Glycine max)	642:662	Thus, a greenhouse experiment was conducted to assess the fertilizing effect of synthetic apatite nanoparticles on soybean (Glycine max).
25023201	3	20	from	effect	597:602	arg1	max					659:661	Glycine max	651:661	Glycine max	651:661	Thus, a greenhouse experiment was conducted to assess the fertilizing effect of synthetic apatite nanoparticles on soybean (Glycine max).
25023201	1	21	theme	phosphorus	164:173	arg1	fertilizers					179:189	highly effective phosphorus (P) fertilizers	147:189	highly effective phosphorus (P) fertilizers	147:189	Some soluble phosphate salts, heavily used in agriculture as highly effective phosphorus (P) fertilizers, cause surface water eutrophication, while solid phosphates are less effective in supplying the nutrient P.
25023201	0	22	theme	Synthetic	0:8	arg1	apatite					10:16	Synthetic apatite	0:16	Synthetic apatite	0:16	Synthetic apatite nanoparticles as a phosphorus fertilizer for soybean (Glycine max).
25023201	4	23	theme	chemical	789:796	arg1	composition					798:808	the chemical composition	785:808	the chemical composition	785:808	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	4	23	theme	chemical	789:796	arg1	hydroxyapatite					819:832	pure hydroxyapatite	814:832	pure hydroxyapatite	814:832	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	3	24	theme	greenhouse	535:544	arg1	experiment					546:555	a greenhouse experiment	533:555	a greenhouse experiment	533:555	Thus, a greenhouse experiment was conducted to assess the fertilizing effect of synthetic apatite nanoparticles on soybean (Glycine max).
25023201	7	25	theme	fertilizer	1174:1183	arg1	class					1163:1167	a new class	1157:1167	a new class of P fertilizer	1157:1183	Using apatite nanoparticles as a new class of P fertilizer can potentially enhance agronomical yield and reduce risks of water eutrophication.
25023201	7	25	theme	fertilizer	1174:1183	arg1	nanoparticles					1140:1152	apatite nanoparticles	1132:1152	apatite nanoparticles	1132:1152	Using apatite nanoparticles as a new class of P fertilizer can potentially enhance agronomical yield and reduce risks of water eutrophication.
25023201	4	26	with	spherical	730:738	arg1	diameters					754:762	diameters	754:762	diameters of 15.8 ± 7.4 nm	754:779	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	5	27	theme	growth	901:906	arg1	rate					908:911	the growth rate	897:911	the growth rate	897:911	The data show that application of the nanoparticles increased the growth rate and seed yield by 32.6% and 20.4%, respectively, compared to those of soybeans treated with a regular P fertilizer (Ca(H2PO4)2).
25023201	4	28	theme	one-step	695:702	arg1	method					717:722	one-step wet chemical method	695:722	one-step wet chemical method	695:722	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	3	29	theme	apatite	617:623	arg1	nanoparticles					625:637	synthetic apatite nanoparticles	607:637	synthetic apatite nanoparticles	607:637	Thus, a greenhouse experiment was conducted to assess the fertilizing effect of synthetic apatite nanoparticles on soybean (Glycine max).
25023201	1	30	theme	soluble	91:97	arg1	salts					109:113	Some soluble phosphate salts	86:113	Some soluble phosphate salts	86:113	Some soluble phosphate salts, heavily used in agriculture as highly effective phosphorus (P) fertilizers, cause surface water eutrophication, while solid phosphates are less effective in supplying the nutrient P.
25023201	0	31	theme	phosphorus	37:46	arg1	fertilizer					48:57	a phosphorus fertilizer	35:57	a phosphorus fertilizer for soybean (Glycine max)	35:83	Synthetic apatite nanoparticles as a phosphorus fertilizer for soybean (Glycine max).
25023201	2	32	theme	soluble	505:511	arg1	counterparts					513:524	the soluble counterparts	501:524	the soluble counterparts	501:524	In contrast, synthetic apatite nanoparticles could hypothetically supply sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae in comparison to the soluble counterparts.
25023201	3	33	theme	nanoparticles	625:637	arg1	effect					597:602	the fertilizing effect	581:602	the fertilizing effect of synthetic apatite nanoparticles on soybean (Glycine max)	581:662	Thus, a greenhouse experiment was conducted to assess the fertilizing effect of synthetic apatite nanoparticles on soybean (Glycine max).
25023201	7	34	theme	water	1247:1251	arg1	eutrophication					1253:1266	water eutrophication	1247:1266	water eutrophication	1247:1266	Using apatite nanoparticles as a new class of P fertilizer can potentially enhance agronomical yield and reduce risks of water eutrophication.
25023201	2	35	from	supply	365:370	arg1	contrast					302:309	contrast	302:309	contrast	302:309	In contrast, synthetic apatite nanoparticles could hypothetically supply sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae in comparison to the soluble counterparts.
25023201	2	35	from	supply	365:370	arg1	comparison					487:496	comparison	487:496	comparison to the soluble counterparts	487:524	In contrast, synthetic apatite nanoparticles could hypothetically supply sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae in comparison to the soluble counterparts.
25023201	7	36	theme	new	1159:1161	arg1	class					1163:1167	a new class	1157:1167	a new class of P fertilizer	1157:1183	Using apatite nanoparticles as a new class of P fertilizer can potentially enhance agronomical yield and reduce risks of water eutrophication.
25023201	7	36	theme	new	1159:1161	arg1	nanoparticles					1140:1152	apatite nanoparticles	1132:1152	apatite nanoparticles	1132:1152	Using apatite nanoparticles as a new class of P fertilizer can potentially enhance agronomical yield and reduce risks of water eutrophication.
25023201	3	37	theme	synthetic	607:615	arg1	nanoparticles					625:637	synthetic apatite nanoparticles	607:637	synthetic apatite nanoparticles	607:637	Thus, a greenhouse experiment was conducted to assess the fertilizing effect of synthetic apatite nanoparticles on soybean (Glycine max).
25023201	4	38	theme	nm	778:779	arg1	diameters					754:762	diameters	754:762	diameters of 15.8 ± 7.4 nm	754:779	The particles, prepared using one-step wet chemical method, were spherical in shape with diameters of 15.8 ± 7.4 nm and the chemical composition was pure hydroxyapatite.
25023201	1	39	theme	phosphate	99:107	arg1	salts					109:113	Some soluble phosphate salts	86:113	Some soluble phosphate salts	86:113	Some soluble phosphate salts, heavily used in agriculture as highly effective phosphorus (P) fertilizers, cause surface water eutrophication, while solid phosphates are less effective in supplying the nutrient P.
25023201	1	40	theme	nutrient	287:294	arg1	P					296:296	the nutrient P	283:296	the nutrient P	283:296	Some soluble phosphate salts, heavily used in agriculture as highly effective phosphorus (P) fertilizers, cause surface water eutrophication, while solid phosphates are less effective in supplying the nutrient P.
25023201	3	41	theme	Glycine	651:657	arg1	soybean					642:648	soybean	642:648	soybean (Glycine max)	642:662	Thus, a greenhouse experiment was conducted to assess the fertilizing effect of synthetic apatite nanoparticles on soybean (Glycine max).
25023201	3	41	theme	Glycine	651:657	arg1	max					659:661	Glycine max	651:661	Glycine max	651:661	Thus, a greenhouse experiment was conducted to assess the fertilizing effect of synthetic apatite nanoparticles on soybean (Glycine max).
25023201	3	42	theme	fertilizing	585:595	arg1	effect					597:602	the fertilizing effect	581:602	the fertilizing effect of synthetic apatite nanoparticles on soybean (Glycine max)	581:662	Thus, a greenhouse experiment was conducted to assess the fertilizing effect of synthetic apatite nanoparticles on soybean (Glycine max).
25023201	7	43	theme	eutrophication	1253:1266	arg1	risks					1238:1242	risks	1238:1242	risks of water eutrophication	1238:1266	Using apatite nanoparticles as a new class of P fertilizer can potentially enhance agronomical yield and reduce risks of water eutrophication.
25023201	5	44	theme	nanoparticles	873:885	arg1	application					854:864	application	854:864	application of the nanoparticles	854:885	The data show that application of the nanoparticles increased the growth rate and seed yield by 32.6% and 20.4%, respectively, compared to those of soybeans treated with a regular P fertilizer (Ca(H2PO4)2).
25023201	1	45	theme	surface	198:204	arg1	eutrophication					212:225	surface water eutrophication	198:225	surface water eutrophication	198:225	Some soluble phosphate salts, heavily used in agriculture as highly effective phosphorus (P) fertilizers, cause surface water eutrophication, while solid phosphates are less effective in supplying the nutrient P.
25023201	2	46	theme	sufficient	372:381	arg1	nutrients					385:393	sufficient P nutrients	372:393	sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae	372:482	In contrast, synthetic apatite nanoparticles could hypothetically supply sufficient P nutrients to crops but with less mobility in the environment and with less bioavailable P to algae in comparison to the soluble counterparts.
25023201	1	47	theme	water	206:210	arg1	eutrophication					212:225	surface water eutrophication	198:225	surface water eutrophication	198:225	Some soluble phosphate salts, heavily used in agriculture as highly effective phosphorus (P) fertilizers, cause surface water eutrophication, while solid phosphates are less effective in supplying the nutrient P.
25023201	5	48	theme	Ca	1029:1030	arg1	fertilizer					1017:1026	a regular P fertilizer	1005:1026	a regular P fertilizer (Ca(H2PO4)2)	1005:1039	The data show that application of the nanoparticles increased the growth rate and seed yield by 32.6% and 20.4%, respectively, compared to those of soybeans treated with a regular P fertilizer (Ca(H2PO4)2).
25023201	5	48	theme	Ca	1029:1030	arg1	H2PO4					1032:1036	Ca(H2PO4)2	1029:1038	Ca(H2PO4)2	1029:1038	The data show that application of the nanoparticles increased the growth rate and seed yield by 32.6% and 20.4%, respectively, compared to those of soybeans treated with a regular P fertilizer (Ca(H2PO4)2).
25023201	7	49	theme	agronomical	1209:1219	arg1	yield					1221:1225	agronomical yield	1209:1225	agronomical yield	1209:1225	Using apatite nanoparticles as a new class of P fertilizer can potentially enhance agronomical yield and reduce risks of water eutrophication.
26093475	11	0	theme	territorial	1412:1422	arg1	matrix					1424:1429	the pericellular and territorial matrix	1391:1429	the pericellular and territorial matrix	1391:1429	At 7 days, basophilia was observed in the pericellular and territorial matrix, which decreased in the latter over the period studied.
26093475	19	1	theme	cartilage	2369:2377	arg1	occurrence					2406:2415	the occurrence	2402:2415	the occurrence of age-related changes in the structural organization and composition of the extracellular matrix	2402:2513	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	19	1	theme	cartilage	2369:2377	arg1	nature					2345:2350	the elastic nature	2333:2350	the elastic nature of the arytenoid cartilage of L. catesbeianus	2333:2396	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	17	2	theme	glycosaminoglycans	2004:2021	arg1	higher					2027:2032	higher	2027:2032	higher	2027:2032	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	17	2	theme	glycosaminoglycans	2004:2021	arg1	amount					1988:1993	The amount	1984:1993	The amount of total glycosaminoglycans	1984:2021	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	17	2	theme	glycosaminoglycans	2004:2021	arg1	glycosaminoglycans					2004:2021	total glycosaminoglycans	1998:2021	total glycosaminoglycans	1998:2021	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	12	3	theme	Collagen	1487:1494	arg1	fibers					1496:1501	Collagen fibers	1487:1501	Collagen fibers	1487:1501	Collagen fibers were arranged perpendicular to the major axis of the cartilaginous plate and were thicker in older animals.
26093475	13	4	from	specimens	1683:1691	arg1	animals					1709:1715	1080-day-old animals	1696:1715	1080-day-old animals	1696:1715	Few calcification areas were observed at the periphery of the cartilage specimens in 1080-day-old animals.
26093475	9	5	theme	methylene	1229:1237	arg1	methods					1276:1282	the dimethyl methylene blue and agarose gel electrophoresis methods	1216:1282	the dimethyl methylene blue and agarose gel electrophoresis methods	1216:1282	For quantification and analysis of glycosaminoglycans were used the dimethyl methylene blue and agarose gel electrophoresis methods.
26093475	11	6	located	observed	1379:1386	arg1	matrix					1424:1429	the pericellular and territorial matrix	1391:1429	the pericellular and territorial matrix	1391:1429	At 7 days, basophilia was observed in the pericellular and territorial matrix, which decreased in the latter over the period studied.
26093475	11	6	located	observed	1379:1386	arg1	days					1358:1361	7 days	1356:1361	7 days	1356:1361	At 7 days, basophilia was observed in the pericellular and territorial matrix, which decreased in the latter over the period studied.
26093475	11	6	located	observed	1379:1386	arg2	basophilia					1364:1373	basophilia	1364:1373	basophilia	1364:1373	At 7 days, basophilia was observed in the pericellular and territorial matrix, which decreased in the latter over the period studied.
26093475	17	7	theme	total	1998:2002	arg1	glycosaminoglycans					2004:2021	total glycosaminoglycans	1998:2021	total glycosaminoglycans	1998:2021	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	19	8	from	nature	2345:2350	arg1	composition					2475:2485	composition	2475:2485	composition	2475:2485	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	19	8	from	nature	2345:2350	arg1	organization					2458:2469	structural organization	2447:2469	structural organization	2447:2469	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	2	9	theme	aging	300:304	arg1	effects					289:295	the effects	285:295	the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes	285:498	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	6	10	theme	ultrastructural	943:957	arg1	analysis					976:983	the ultrastructural and cytochemical analysis	939:983	the ultrastructural and cytochemical analysis of proteoglycans	939:1000	Other samples were processed for the ultrastructural and cytochemical analysis of proteoglycans.
26093475	17	11	theme	chondroitin-	2109:2120	arg1	presence					2097:2104	marked presence	2090:2104	marked presence of chondroitin- and dermatan-sulfate especially in this age	2090:2164	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	15	12	located	found	1812:1816	arg1	stroma					1825:1830	stroma	1825:1830	stroma	1825:1830	Elastic fibers were found in the stroma and perichondrium and increased with age in the two regions.
26093475	15	12	located	found	1812:1816	arg1	perichondrium					1836:1848	perichondrium	1836:1848	perichondrium	1836:1848	Elastic fibers were found in the stroma and perichondrium and increased with age in the two regions.
26093475	15	12	located	found	1812:1816	arg2	fibers					1800:1805	Elastic fibers	1792:1805	Elastic fibers	1792:1805	Elastic fibers were found in the stroma and perichondrium and increased with age in the two regions.
26093475	9	13	theme	gel	1256:1258	arg1	electrophoresis					1260:1274	agarose gel electrophoresis	1248:1274	agarose gel electrophoresis	1248:1274	For quantification and analysis of glycosaminoglycans were used the dimethyl methylene blue and agarose gel electrophoresis methods.
26093475	13	14	theme	calcification	1615:1627	arg1	areas					1629:1633	Few calcification areas	1611:1633	Few calcification areas	1611:1633	Few calcification areas were observed at the periphery of the cartilage specimens in 1080-day-old animals.
26093475	19	15	theme	age-related	2420:2430	arg1	changes					2432:2438	age-related changes	2420:2438	age-related changes in the structural organization and composition of the extracellular matrix	2420:2513	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	20	16	theme	larynx	2574:2579	arg1	function					2558:2565	the function	2554:2565	the function of the larynx in the animal	2554:2593	These changes may contribute to alter the function of the larynx in the animal during aging.
26093475	17	17	theme	dermatan-sulfate	2126:2141	arg1	presence					2097:2104	marked presence	2090:2104	marked presence of chondroitin- and dermatan-sulfate especially in this age	2090:2164	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	11	18	theme	pericellular	1395:1406	arg1	matrix					1424:1429	the pericellular and territorial matrix	1391:1429	the pericellular and territorial matrix	1391:1429	At 7 days, basophilia was observed in the pericellular and territorial matrix, which decreased in the latter over the period studied.
26093475	18	19	theme	total	2215:2219	arg1	concentration					2230:2242	the total collagen concentration	2211:2242	the total collagen concentration	2211:2242	The content of hydroxyproline, which infers the total collagen concentration, was higher in 1080-day-old animals compared to the other ages.
26093475	17	20	theme	180-day-old	2037:2047	arg1	animals					2049:2055	180-day-old animals	2037:2055	180-day-old animals	2037:2055	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	6	21	theme	Other	906:910	arg1	samples					912:918	Other samples	906:918	Other samples	906:918	Other samples were processed for the ultrastructural and cytochemical analysis of proteoglycans.
26093475	2	22	theme	study	263:267	arg1	objective					245:253	The objective	241:253	The objective of this study	241:267	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	3	23	from	days	528:531	arg1	Animals					501:507	Animals	501:507	Animals at 7, 180 and 1080 days post-metamorphosis (n=10/age)	501:561	Animals at 7, 180 and 1080 days post-metamorphosis (n=10/age) were euthanized and the cartilage was removed and processed for structural and biochemical analysis.
26093475	1	24	theme	aging	124:128	arg1	process					130:136	The aging process	120:136	The aging process	120:136	The aging process induces progressive and irreversible changes in the structural and functional organization of animals.
26093475	2	25	theme	commercial	480:489	arg1	purposes					491:498	commercial purposes	480:498	commercial purposes	480:498	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	19	26	from	occurrence	2406:2415	arg1	composition					2475:2485	composition	2475:2485	composition	2475:2485	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	19	26	from	occurrence	2406:2415	arg1	organization					2458:2469	structural organization	2447:2469	structural organization	2447:2469	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	15	27	theme	Elastic	1792:1798	arg1	fibers					1800:1805	Elastic fibers	1792:1805	Elastic fibers	1792:1805	Elastic fibers were found in the stroma and perichondrium and increased with age in the two regions.
26093475	0	28	theme	bullfrog	56:63	arg1	cartilage					39:47	arytenoid cartilage	29:47	arytenoid cartilage of the bullfrog (Lithobates catesbeianus)	29:89	Structure and composition of arytenoid cartilage of the bullfrog (Lithobates catesbeianus) during maturation and aging.
26093475	19	29	theme	structural	2447:2456	arg1	organization					2458:2469	structural organization	2447:2469	structural organization	2447:2469	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	4	30	theme	structural	672:681	arg1	analyses					683:690	the structural analyses	668:690	the structural analyses	668:690	For the structural analyses, cartilage sections were stained with picrosirius, toluidine blue, Weigert's resorcin-fuchsin and Von Kossa stain.
26093475	10	31	theme	T	1300:1300	arg1	method					1302:1307	The chloramine T method	1285:1307	The chloramine T method	1285:1307	The chloramine T method was used for hydroxyproline quantification.
26093475	2	32	dep	Lithobates	433:442	arg1	catesbeianus					444:455	Lithobates catesbeianus	433:455	Lithobates catesbeianus	433:455	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	5	33	theme	types	890:894	arg1	detection					868:876	detection	868:876	detection of collagen types I and II	868:903	The sections were also submitted to immunohistochemistry for detection of collagen types I and II.
26093475	6	34	theme	cytochemical	963:974	arg1	analysis					976:983	the ultrastructural and cytochemical analysis	939:983	the ultrastructural and cytochemical analysis of proteoglycans	939:1000	Other samples were processed for the ultrastructural and cytochemical analysis of proteoglycans.
26093475	2	35	theme	cartilage	384:392	arg1	matrix					360:365	the extracellular matrix	342:365	the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes	342:498	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	18	36	theme	hydroxyproline	2182:2195	arg1	content					2171:2177	The content	2167:2177	The content	2167:2177	The content of hydroxyproline, which infers the total collagen concentration, was higher in 1080-day-old animals compared to the other ages.
26093475	18	36	theme	hydroxyproline	2182:2195	arg1	higher					2249:2254	higher	2249:2254	higher	2249:2254	The content of hydroxyproline, which infers the total collagen concentration, was higher in 1080-day-old animals compared to the other ages.
26093475	20	37	from	function	2558:2565	arg1	animal					2588:2593	the animal	2584:2593	the animal	2584:2593	These changes may contribute to alter the function of the larynx in the animal during aging.
26093475	10	38	used	used	1313:1316	arg2	method					1302:1307	The chloramine T method	1285:1307	The chloramine T method	1285:1307	The chloramine T method was used for hydroxyproline quantification.
26093475	2	39	dep	structure	313:321	arg1	the					309:311	the	309:311	the	309:311	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	0	40	dep	Lithobates	66:75	arg1	catesbeianus					77:88	Lithobates catesbeianus	66:88	Lithobates catesbeianus	66:88	Structure and composition of arytenoid cartilage of the bullfrog (Lithobates catesbeianus) during maturation and aging.
26093475	18	41	theme	other	2296:2300	arg1	ages					2302:2305	the other ages	2292:2305	the other ages	2292:2305	The content of hydroxyproline, which infers the total collagen concentration, was higher in 1080-day-old animals compared to the other ages.
26093475	2	42	theme	male	417:420	arg1	bullfrogs					422:430	male bullfrogs	417:430	male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes	417:498	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	2	42	theme	male	417:420	arg1	Lithobates					433:442	Lithobates	433:442	Lithobates	433:442	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	13	43	theme	cartilage	1673:1681	arg1	specimens					1683:1691	the cartilage specimens	1669:1691	the cartilage specimens in 1080-day-old animals	1669:1715	Few calcification areas were observed at the periphery of the cartilage specimens in 1080-day-old animals.
26093475	4	44	theme	picrosirius	730:740	arg1	resorcin-fuchsin					769:784	picrosirius, toluidine blue, Weigert's resorcin-fuchsin	730:784	resorcin-fuchsin	769:784	For the structural analyses, cartilage sections were stained with picrosirius, toluidine blue, Weigert's resorcin-fuchsin and Von Kossa stain.
26093475	13	45	from	animals	1709:1715	arg1	periphery					1656:1664	the periphery	1652:1664	the periphery of the cartilage specimens in 1080-day-old animals	1652:1715	Few calcification areas were observed at the periphery of the cartilage specimens in 1080-day-old animals.
26093475	8	46	theme	positive	1071:1078	arg1	stainings					1080:1088	positive stainings	1071:1088	positive stainings for proteoglycans	1071:1106	The number of positive stainings for proteoglycans was quantified by ultrastructural analysis.
26093475	12	47	theme	major	1538:1542	arg1	axis					1544:1547	the major axis	1534:1547	the major axis of the cartilaginous plate	1534:1574	Collagen fibers were arranged perpendicular to the major axis of the cartilaginous plate and were thicker in older animals.
26093475	4	48	theme	Kossa	794:798	arg1	stain					800:804	Von Kossa stain	790:804	Von Kossa stain	790:804	For the structural analyses, cartilage sections were stained with picrosirius, toluidine blue, Weigert's resorcin-fuchsin and Von Kossa stain.
26093475	16	49	theme	Proteoglycan	1893:1904	arg1	staining					1906:1913	Proteoglycan staining	1893:1913	Proteoglycan staining	1893:1913	Proteoglycan staining significantly increased from 7 to 180 days and reduced at 1080 days.
26093475	19	50	theme	extracellular	2494:2506	arg1	matrix					2508:2513	the extracellular matrix	2490:2513	the extracellular matrix	2490:2513	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	0	51	theme	arytenoid	29:37	arg1	cartilage					39:47	arytenoid cartilage	29:47	arytenoid cartilage of the bullfrog (Lithobates catesbeianus)	29:89	Structure and composition of arytenoid cartilage of the bullfrog (Lithobates catesbeianus) during maturation and aging.
26093475	14	52	attach	present	1739:1745	arg1	ages					1786:1789	the different ages	1772:1789	the different ages	1772:1789	Type II collagen was present throughout the stroma at the different ages.
26093475	14	52	attach	present	1739:1745	arg2	collagen					1726:1733	Type II collagen	1718:1733	Type II collagen	1718:1733	Type II collagen was present throughout the stroma at the different ages.
26093475	19	53	theme	arytenoid	2359:2367	arg1	cartilage					2369:2377	the arytenoid cartilage	2355:2377	the arytenoid cartilage of L. catesbeianus	2355:2396	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	2	54	theme	matrix	360:365	arg1	composition					327:337	composition	327:337	composition	327:337	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	2	54	theme	matrix	360:365	arg1	structure					313:321	structure	313:321	structure	313:321	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	19	55	dep	organization	2458:2469	arg1	the					2443:2445	the	2443:2445	the	2443:2445	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	9	56	theme	glycosaminoglycans	1187:1204	arg1	analysis					1175:1182	analysis	1175:1182	analysis	1175:1182	For quantification and analysis of glycosaminoglycans were used the dimethyl methylene blue and agarose gel electrophoresis methods.
26093475	9	56	theme	glycosaminoglycans	1187:1204	arg1	quantification					1156:1169	quantification	1156:1169	quantification	1156:1169	For quantification and analysis of glycosaminoglycans were used the dimethyl methylene blue and agarose gel electrophoresis methods.
26093475	19	57	theme	catesbeianus	2385:2396	arg1	cartilage					2369:2377	the arytenoid cartilage	2355:2377	the arytenoid cartilage of L. catesbeianus	2355:2396	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	13	58	located	observed	1640:1647	arg1	periphery					1656:1664	the periphery	1652:1664	the periphery of the cartilage specimens in 1080-day-old animals	1652:1715	Few calcification areas were observed at the periphery of the cartilage specimens in 1080-day-old animals.
26093475	13	58	located	observed	1640:1647	arg2	areas					1629:1633	Few calcification areas	1611:1633	Few calcification areas	1611:1633	Few calcification areas were observed at the periphery of the cartilage specimens in 1080-day-old animals.
26093475	5	59	theme	collagen	881:888	arg1	types					890:894	collagen types I and II	881:903	collagen types I and II	881:903	The sections were also submitted to immunohistochemistry for detection of collagen types I and II.
26093475	5	59	theme	collagen	881:888	arg1	II					902:903	II	902:903	II	902:903	The sections were also submitted to immunohistochemistry for detection of collagen types I and II.
26093475	5	59	theme	collagen	881:888	arg1	I					896:896	I	896:896	I	896:896	The sections were also submitted to immunohistochemistry for detection of collagen types I and II.
26093475	9	60	theme	dimethyl	1220:1227	arg1	methods					1276:1282	the dimethyl methylene blue and agarose gel electrophoresis methods	1216:1282	the dimethyl methylene blue and agarose gel electrophoresis methods	1216:1282	For quantification and analysis of glycosaminoglycans were used the dimethyl methylene blue and agarose gel electrophoresis methods.
26093475	3	61	theme	7	512:512	arg1	days					528:531	7, 180 and 1080 days post-metamorphosis (n=10/age)	512:561	days	528:531	Animals at 7, 180 and 1080 days post-metamorphosis (n=10/age) were euthanized and the cartilage was removed and processed for structural and biochemical analysis.
26093475	3	61	theme	7	512:512	arg1	n=10/age					553:560	n=10/age	553:560	n=10/age	553:560	Animals at 7, 180 and 1080 days post-metamorphosis (n=10/age) were euthanized and the cartilage was removed and processed for structural and biochemical analysis.
26093475	1	62	theme	irreversible	162:173	arg1	changes					175:181	progressive and irreversible changes	146:181	progressive and irreversible changes	146:181	The aging process induces progressive and irreversible changes in the structural and functional organization of animals.
26093475	12	63	theme	cartilaginous	1556:1568	arg1	plate					1570:1574	the cartilaginous plate	1552:1574	the cartilaginous plate	1552:1574	Collagen fibers were arranged perpendicular to the major axis of the cartilaginous plate and were thicker in older animals.
26093475	17	64	theme	marked	2090:2095	arg1	presence					2097:2104	marked presence	2090:2104	marked presence of chondroitin- and dermatan-sulfate especially in this age	2090:2164	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	9	65	theme	blue	1239:1242	arg1	methylene					1229:1237	methylene blue	1229:1242	methylene blue	1229:1242	For quantification and analysis of glycosaminoglycans were used the dimethyl methylene blue and agarose gel electrophoresis methods.
26093475	3	66	theme	post-metamorphosis	533:550	arg1	days					528:531	7, 180 and 1080 days post-metamorphosis (n=10/age)	512:561	days	528:531	Animals at 7, 180 and 1080 days post-metamorphosis (n=10/age) were euthanized and the cartilage was removed and processed for structural and biochemical analysis.
26093475	3	66	theme	post-metamorphosis	533:550	arg1	n=10/age					553:560	n=10/age	553:560	n=10/age	553:560	Animals at 7, 180 and 1080 days post-metamorphosis (n=10/age) were euthanized and the cartilage was removed and processed for structural and biochemical analysis.
26093475	9	67	theme	agarose	1248:1254	arg1	electrophoresis					1260:1274	agarose gel electrophoresis	1248:1274	agarose gel electrophoresis	1248:1274	For quantification and analysis of glycosaminoglycans were used the dimethyl methylene blue and agarose gel electrophoresis methods.
26093475	13	68	theme	Few	1611:1613	arg1	areas					1629:1633	Few calcification areas	1611:1633	Few calcification areas	1611:1633	Few calcification areas were observed at the periphery of the cartilage specimens in 1080-day-old animals.
26093475	1	69	theme	structural	190:199	arg1	organization					216:227	the structural and functional organization	186:227	the structural and functional organization of animals	186:238	The aging process induces progressive and irreversible changes in the structural and functional organization of animals.
26093475	9	70	theme	electrophoresis	1260:1274	arg1	methods					1276:1282	the dimethyl methylene blue and agarose gel electrophoresis methods	1216:1282	the dimethyl methylene blue and agarose gel electrophoresis methods	1216:1282	For quantification and analysis of glycosaminoglycans were used the dimethyl methylene blue and agarose gel electrophoresis methods.
26093475	19	71	theme	changes	2432:2438	arg1	occurrence					2406:2415	the occurrence	2402:2415	the occurrence of age-related changes in the structural organization and composition of the extracellular matrix	2402:2513	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	19	71	theme	changes	2432:2438	arg1	nature					2345:2350	the elastic nature	2333:2350	the elastic nature of the arytenoid cartilage of L. catesbeianus	2333:2396	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	1	72	theme	functional	205:214	arg1	organization					216:227	the structural and functional organization	186:227	the structural and functional organization of animals	186:238	The aging process induces progressive and irreversible changes in the structural and functional organization of animals.
26093475	7	73	used	used	1030:1033	arg2	sections					1016:1023	Histological sections	1003:1023	Histological sections	1003:1023	Histological sections were used to chondrocyte count.
26093475	2	74	located	found	394:398	arg2	cartilage					384:392	the arytenoid cartilage	370:392	the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes	370:498	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	2	74	located	found	394:398	arg1	larynx					407:412	the larynx	403:412	the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes	403:498	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	19	75	from	changes	2432:2438	arg1	composition					2475:2485	composition	2475:2485	composition	2475:2485	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	19	75	from	changes	2432:2438	arg1	organization					2458:2469	structural organization	2447:2469	structural organization	2447:2469	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	15	76	dep	stroma	1825:1830	arg1	the					1821:1823	the	1821:1823	the	1821:1823	Elastic fibers were found in the stroma and perichondrium and increased with age in the two regions.
26093475	4	77	dep	picrosirius	730:740	arg1	blue					753:756	blue	753:756	blue	753:756	For the structural analyses, cartilage sections were stained with picrosirius, toluidine blue, Weigert's resorcin-fuchsin and Von Kossa stain.
26093475	18	78	theme	collagen	2221:2228	arg1	concentration					2230:2242	the total collagen concentration	2211:2242	the total collagen concentration	2211:2242	The content of hydroxyproline, which infers the total collagen concentration, was higher in 1080-day-old animals compared to the other ages.
26093475	14	79	theme	Type	1718:1721	arg1	collagen					1726:1733	Type II collagen	1718:1733	Type II collagen	1718:1733	Type II collagen was present throughout the stroma at the different ages.
26093475	14	80	located	present	1739:1745	arg1	ages					1786:1789	the different ages	1772:1789	the different ages	1772:1789	Type II collagen was present throughout the stroma at the different ages.
26093475	14	80	located	present	1739:1745	arg2	collagen					1726:1733	Type II collagen	1718:1733	Type II collagen	1718:1733	Type II collagen was present throughout the stroma at the different ages.
26093475	7	81	theme	Histological	1003:1014	arg1	sections					1016:1023	Histological sections	1003:1023	Histological sections	1003:1023	Histological sections were used to chondrocyte count.
26093475	3	82	theme	1080	523:526	arg1	days					528:531	7, 180 and 1080 days post-metamorphosis (n=10/age)	512:561	days	528:531	Animals at 7, 180 and 1080 days post-metamorphosis (n=10/age) were euthanized and the cartilage was removed and processed for structural and biochemical analysis.
26093475	3	82	theme	1080	523:526	arg1	n=10/age					553:560	n=10/age	553:560	n=10/age	553:560	Animals at 7, 180 and 1080 days post-metamorphosis (n=10/age) were euthanized and the cartilage was removed and processed for structural and biochemical analysis.
26093475	4	83	theme	cartilage	693:701	arg1	sections					703:710	cartilage sections	693:710	cartilage sections	693:710	For the structural analyses, cartilage sections were stained with picrosirius, toluidine blue, Weigert's resorcin-fuchsin and Von Kossa stain.
26093475	14	84	from	ages	1786:1789	arg1	present					1739:1745	present	1739:1745	present	1739:1745	Type II collagen was present throughout the stroma at the different ages.
26093475	12	85	theme	older	1596:1600	arg1	animals					1602:1608	older animals	1596:1608	older animals	1596:1608	Collagen fibers were arranged perpendicular to the major axis of the cartilaginous plate and were thicker in older animals.
26093475	16	86	dep	180	1949:1951	arg1	to					1946:1947	to	1946:1947	to	1946:1947	Proteoglycan staining significantly increased from 7 to 180 days and reduced at 1080 days.
26093475	17	87	theme	other	2073:2077	arg1	ages					2079:2082	the other ages	2069:2082	the other ages	2069:2082	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	1	88	theme	progressive	146:156	arg1	changes					175:181	progressive and irreversible changes	146:181	progressive and irreversible changes	146:181	The aging process induces progressive and irreversible changes in the structural and functional organization of animals.
26093475	2	89	from	effects	289:295	arg1	composition					327:337	composition	327:337	composition	327:337	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	2	89	from	effects	289:295	arg1	structure					313:321	structure	313:321	structure	313:321	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	6	90	theme	proteoglycans	988:1000	arg1	analysis					976:983	the ultrastructural and cytochemical analysis	939:983	the ultrastructural and cytochemical analysis of proteoglycans	939:1000	Other samples were processed for the ultrastructural and cytochemical analysis of proteoglycans.
26093475	10	91	theme	chloramine	1289:1298	arg1	method					1302:1307	The chloramine T method	1285:1307	The chloramine T method	1285:1307	The chloramine T method was used for hydroxyproline quantification.
26093475	10	92	theme	hydroxyproline	1322:1335	arg1	quantification					1337:1350	hydroxyproline quantification	1322:1350	hydroxyproline quantification	1322:1350	The chloramine T method was used for hydroxyproline quantification.
26093475	12	93	theme	plate	1570:1574	arg1	axis					1544:1547	the major axis	1534:1547	the major axis of the cartilaginous plate	1534:1574	Collagen fibers were arranged perpendicular to the major axis of the cartilaginous plate and were thicker in older animals.
26093475	19	94	from	organization	2458:2469	arg1	occurrence					2406:2415	the occurrence	2402:2415	the occurrence of age-related changes in the structural organization and composition of the extracellular matrix	2402:2513	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	19	94	from	organization	2458:2469	arg1	nature					2345:2350	the elastic nature	2333:2350	the elastic nature of the arytenoid cartilage of L. catesbeianus	2333:2396	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	2	95	theme	arytenoid	374:382	arg1	cartilage					384:392	the arytenoid cartilage	370:392	the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes	370:498	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	19	96	theme	elastic	2337:2343	arg1	nature					2345:2350	the elastic nature	2333:2350	the elastic nature of the arytenoid cartilage of L. catesbeianus	2333:2396	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	2	97	theme	bullfrogs	422:430	arg1	larynx					407:412	the larynx	403:412	the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes	403:498	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	19	98	from	composition	2475:2485	arg1	occurrence					2406:2415	the occurrence	2402:2415	the occurrence of age-related changes in the structural organization and composition of the extracellular matrix	2402:2513	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	19	98	from	composition	2475:2485	arg1	nature					2345:2350	the elastic nature	2333:2350	the elastic nature of the arytenoid cartilage of L. catesbeianus	2333:2396	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	5	99	dep	types	890:894	arg1	types					890:894	collagen types I and II	881:903	collagen types I and II	881:903	The sections were also submitted to immunohistochemistry for detection of collagen types I and II.
26093475	5	99	dep	types	890:894	arg1	II					902:903	II	902:903	II	902:903	The sections were also submitted to immunohistochemistry for detection of collagen types I and II.
26093475	5	99	dep	types	890:894	arg1	I					896:896	I	896:896	I	896:896	The sections were also submitted to immunohistochemistry for detection of collagen types I and II.
26093475	17	100	from	age	2162:2164	arg1	presence					2097:2104	marked presence	2090:2104	marked presence of chondroitin- and dermatan-sulfate especially in this age	2090:2164	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	8	101	theme	stainings	1080:1088	arg1	number					1061:1066	The number	1057:1066	The number of positive stainings for proteoglycans	1057:1106	The number of positive stainings for proteoglycans was quantified by ultrastructural analysis.
26093475	13	102	theme	specimens	1683:1691	arg1	periphery					1656:1664	the periphery	1652:1664	the periphery of the cartilage specimens in 1080-day-old animals	1652:1715	Few calcification areas were observed at the periphery of the cartilage specimens in 1080-day-old animals.
26093475	0	103	theme	cartilage	39:47	arg1	composition					14:24	composition	14:24	composition	14:24	Structure and composition of arytenoid cartilage of the bullfrog (Lithobates catesbeianus) during maturation and aging.
26093475	0	103	theme	cartilage	39:47	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and composition of arytenoid cartilage of the bullfrog (Lithobates catesbeianus) during maturation and aging.
26093475	13	104	theme	1080-day-old	1696:1707	arg1	animals					1709:1715	1080-day-old animals	1696:1715	1080-day-old animals	1696:1715	Few calcification areas were observed at the periphery of the cartilage specimens in 1080-day-old animals.
26093475	4	105	theme	Von	790:792	arg1	stain					800:804	Von Kossa stain	790:804	Von Kossa stain	790:804	For the structural analyses, cartilage sections were stained with picrosirius, toluidine blue, Weigert's resorcin-fuchsin and Von Kossa stain.
26093475	17	106	from	dermatan-sulfate	2126:2141	arg1	age					2162:2164	this age	2157:2164	this age	2157:2164	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	19	107	theme	matrix	2508:2513	arg1	composition					2475:2485	composition	2475:2485	composition	2475:2485	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	19	107	theme	matrix	2508:2513	arg1	organization					2458:2469	structural organization	2447:2469	structural organization	2447:2469	The results demonstrated the elastic nature of the arytenoid cartilage of L. catesbeianus and the occurrence of age-related changes in the structural organization and composition of the extracellular matrix.
26093475	18	108	theme	1080-day-old	2259:2270	arg1	animals					2272:2278	1080-day-old animals	2259:2278	1080-day-old animals	2259:2278	The content of hydroxyproline, which infers the total collagen concentration, was higher in 1080-day-old animals compared to the other ages.
26093475	3	109	theme	biochemical	642:652	arg1	analysis					654:661	structural and biochemical analysis	627:661	structural and biochemical analysis	627:661	Animals at 7, 180 and 1080 days post-metamorphosis (n=10/age) were euthanized and the cartilage was removed and processed for structural and biochemical analysis.
26093475	17	110	from	chondroitin-	2109:2120	arg1	age					2162:2164	this age	2157:2164	this age	2157:2164	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	8	111	theme	ultrastructural	1126:1140	arg1	analysis					1142:1149	ultrastructural analysis	1126:1149	ultrastructural analysis	1126:1149	The number of positive stainings for proteoglycans was quantified by ultrastructural analysis.
26093475	3	112	theme	structural	627:636	arg1	analysis					654:661	structural and biochemical analysis	627:661	structural and biochemical analysis	627:661	Animals at 7, 180 and 1080 days post-metamorphosis (n=10/age) were euthanized and the cartilage was removed and processed for structural and biochemical analysis.
26093475	17	113	from	presence	2097:2104	arg1	age					2162:2164	this age	2157:2164	this age	2157:2164	The amount of total glycosaminoglycans was higher in 180-day-old animals compared to the other ages, with marked presence of chondroitin- and dermatan-sulfate especially in this age.
26093475	1	114	theme	animals	232:238	arg1	organization					216:227	the structural and functional organization	186:227	the structural and functional organization of animals	186:238	The aging process induces progressive and irreversible changes in the structural and functional organization of animals.
26093475	13	115	from	periphery	1656:1664	arg1	animals					1709:1715	1080-day-old animals	1696:1715	1080-day-old animals	1696:1715	Few calcification areas were observed at the periphery of the cartilage specimens in 1080-day-old animals.
26093475	2	116	theme	extracellular	346:358	arg1	matrix					360:365	the extracellular matrix	342:365	the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes	342:498	The objective of this study was to evaluate the effects of aging on the structure and composition of the extracellular matrix of the arytenoid cartilage found in the larynx of male bullfrogs (Lithobates catesbeianus) kept in captivity for commercial purposes.
26093475	14	117	theme	different	1776:1784	arg1	ages					1786:1789	the different ages	1772:1789	the different ages	1772:1789	Type II collagen was present throughout the stroma at the different ages.
25477312	3	0	from	synergy	565:571	arg1	management					613:622	caries management	606:622	caries management	606:622	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	2	1	theme	hard-tissue	306:316	arg1	remineralization					318:333	tooth hard-tissue remineralization	300:333	tooth hard-tissue remineralization	300:333	Fluoride is a widely used anticaries agent, which promotes tooth hard-tissue remineralization and suppresses bacterial activities.
25477312	10	2	theme	microbial	1934:1942	arg1	equilibrium					1944:1954	a healthy oral microbial equilibrium	1919:1954	a healthy oral microbial equilibrium	1919:1954	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	9	3	theme	optimal	1619:1625	arg1	combination					1627:1637	the optimal combination	1615:1637	the optimal combination of NaF/arginine	1615:1653	More importantly, the optimal combination of NaF/arginine maintained a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis within the alkalized biofilm.
25477312	10	4	contain	has	1869:1871	arg1	application					1832:1842	the combinatory application	1816:1842	the combinatory application of fluoride and arginine	1816:1867	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	10	4	contain	has	1869:1871	arg2	effect					1897:1902	a potential synergistic effect	1873:1902	a potential synergistic effect	1873:1902	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	6	5	theme	polymerase	1309:1318	arg1	reaction					1326:1333	quantitative polymerase chain reaction	1296:1333	quantitative polymerase chain reaction	1296:1333	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	2	6	theme	bacterial	350:358	arg1	activities					360:369	bacterial activities	350:369	bacterial activities	350:369	Fluoride is a widely used anticaries agent, which promotes tooth hard-tissue remineralization and suppresses bacterial activities.
25477312	5	7	theme	sanguinis	973:981	arg1	cultures					925:932	planktonic and biofilm cultures	902:932	planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays	902:1050	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	3	8	contain	containing	433:442	arg2	arginine					462:469	arginine	462:469	arginine	462:469	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	3	8	contain	containing	433:442	arg1	products					424:431	oral hygiene products	411:431	oral hygiene products containing both fluoride and arginine	411:469	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	3	8	contain	containing	433:442	arg2	fluoride					449:456	fluoride	449:456	fluoride	449:456	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	4	9	theme	fluoride	698:705	arg1	benefit					687:693	the ecological benefit	672:693	the ecological benefit of fluoride	672:705	Here, we hypothesize that arginine may augment the ecological benefit of fluoride by enriching alkali-generating bacteria in the plaque biofilm and thus synergizes with fluoride in controlling dental caries.
25477312	9	10	theme	pressure	1683:1690	arg1	"					1691:1691	a "streptococcal pressure"	1666:1691	a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis	1666:1751	More importantly, the optimal combination of NaF/arginine maintained a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis within the alkalized biofilm.
25477312	3	11	theme	greater	481:487	arg1	effect					500:505	a greater anticaries effect	479:505	a greater anticaries effect	479:505	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	5	12	theme	planktonic	902:911	arg1	cultures					925:932	planktonic and biofilm cultures	902:932	planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays	902:1050	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	6	13	theme	optimal	1057:1063	arg1	combinations					1078:1089	The optimal NaF/arginine combinations	1053:1089	The optimal NaF/arginine combinations	1053:1089	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	5	14	theme	checkerboard	1018:1029	arg1	assays					1045:1050	checkerboard microdilution assays	1018:1050	checkerboard microdilution assays	1018:1050	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	3	15	theme	clinical	379:386	arg1	trials					388:393	Recent clinical trials	372:393	Recent clinical trials	372:393	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	1	16	theme	dental	226:231	arg1	plaque					233:238	the dental plaque	222:238	the dental plaque	222:238	Dental caries is closely associated with the microbial disequilibrium between acidogenic/aciduric pathogens and alkali-generating commensal residents within the dental plaque.
25477312	5	17	from	effects	875:881	arg1	cultures					925:932	planktonic and biofilm cultures	902:932	planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays	902:1050	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	2	18	theme	anticaries	267:276	arg1	agent					278:282	a widely used anticaries agent	253:282	a widely used anticaries agent	253:282	Fluoride is a widely used anticaries agent, which promotes tooth hard-tissue remineralization and suppresses bacterial activities.
25477312	2	18	theme	anticaries	267:276	arg1	Fluoride					241:248	Fluoride	241:248	Fluoride	241:248	Fluoride is a widely used anticaries agent, which promotes tooth hard-tissue remineralization and suppresses bacterial activities.
25477312	9	19	theme	potential	1705:1713	arg1	growth					1715:1720	the potential growth	1701:1720	the potential growth of oral anaerobe P. gingivalis	1701:1751	More importantly, the optimal combination of NaF/arginine maintained a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis within the alkalized biofilm.
25477312	1	20	theme	alkali-generating	177:193	arg1	residents					205:213	alkali-generating commensal residents	177:213	alkali-generating commensal residents	177:213	Dental caries is closely associated with the microbial disequilibrium between acidogenic/aciduric pathogens and alkali-generating commensal residents within the dental plaque.
25477312	6	21	theme	combinatory	1116:1126	arg1	effects					1128:1134	their combinatory effects	1110:1134	their combinatory effects on microbial composition	1110:1159	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	9	22	theme	anaerobe	1730:1737	arg1	gingivalis					1742:1751	oral anaerobe P. gingivalis	1725:1751	oral anaerobe P. gingivalis	1725:1751	More importantly, the optimal combination of NaF/arginine maintained a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis within the alkalized biofilm.
25477312	10	23	theme	fluoride	1847:1854	arg1	application					1832:1842	the combinatory application	1816:1842	the combinatory application of fluoride and arginine	1816:1867	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	9	24	theme	gingivalis	1742:1751	arg1	growth					1715:1720	the potential growth	1701:1720	the potential growth of oral anaerobe P. gingivalis	1701:1751	More importantly, the optimal combination of NaF/arginine maintained a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis within the alkalized biofilm.
25477312	6	25	from	effects	1128:1134	arg1	composition					1149:1159	microbial composition	1139:1159	microbial composition	1139:1159	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	4	26	theme	dental	818:823	arg1	caries					825:830	dental caries	818:830	dental caries	818:830	Here, we hypothesize that arginine may augment the ecological benefit of fluoride by enriching alkali-generating bacteria in the plaque biofilm and thus synergizes with fluoride in controlling dental caries.
25477312	0	27	theme	Combinatorial	0:12	arg1	effects					14:20	Combinatorial effects	0:20	Combinatorial effects of arginine and fluoride on oral bacteria	0:62	Combinatorial effects of arginine and fluoride on oral bacteria.
25477312	3	28	theme	hygiene	416:422	arg1	products					424:431	oral hygiene products	411:431	oral hygiene products containing both fluoride and arginine	411:469	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	10	29	theme	arginine	1860:1867	arg1	application					1832:1842	the combinatory application	1816:1842	the combinatory application of fluoride and arginine	1816:1867	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	1	30	theme	commensal	195:203	arg1	residents					205:213	alkali-generating commensal residents	177:213	alkali-generating commensal residents	177:213	Dental caries is closely associated with the microbial disequilibrium between acidogenic/aciduric pathogens and alkali-generating commensal residents within the dental plaque.
25477312	5	31	theme	combinatory	863:873	arg1	effects					875:881	the combinatory effects	859:881	the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays	859:1050	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	6	32	theme	in	1270:1271	arg1	hybridization					1278:1290	bacterial species-specific fluorescence in situ hybridization	1230:1290	bacterial species-specific fluorescence in situ hybridization	1230:1290	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	10	33	theme	synergistic	1885:1895	arg1	effect					1897:1902	a potential synergistic effect	1873:1902	a potential synergistic effect	1873:1902	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	7	34	theme	planktonic	1428:1437	arg1	cultures					1451:1458	both planktonic and biofilm cultures	1423:1458	cultures	1451:1458	We found that arginine synergized with fluoride in suppressing acidogenic S. mutans in both planktonic and biofilm cultures.
25477312	10	35	theme	oral	1929:1932	arg1	equilibrium					1944:1954	a healthy oral microbial equilibrium	1919:1954	a healthy oral microbial equilibrium	1919:1954	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	4	36	theme	ecological	676:685	arg1	benefit					687:693	the ecological benefit	672:693	the ecological benefit of fluoride	672:705	Here, we hypothesize that arginine may augment the ecological benefit of fluoride by enriching alkali-generating bacteria in the plaque biofilm and thus synergizes with fluoride in controlling dental caries.
25477312	6	37	theme	3-species	1206:1214	arg1	biofilm					1216:1222	single-, dual-, and 3-species biofilm	1186:1222	single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction	1186:1333	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	3	38	theme	caries	606:611	arg1	management					613:622	caries management	606:622	caries management	606:622	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	3	39	contain	possess	471:477	arg2	effect					500:505	a greater anticaries effect	479:505	a greater anticaries effect	479:505	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	3	39	contain	possess	471:477	arg1	products					424:431	oral hygiene products	411:431	oral hygiene products containing both fluoride and arginine	411:469	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	2	40	theme	tooth	300:304	arg1	remineralization					318:333	tooth hard-tissue remineralization	300:333	tooth hard-tissue remineralization	300:333	Fluoride is a widely used anticaries agent, which promotes tooth hard-tissue remineralization and suppresses bacterial activities.
25477312	5	41	theme	mutans	951:956	arg1	cultures					925:932	planktonic and biofilm cultures	902:932	planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays	902:1050	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	6	42	theme	chain	1320:1324	arg1	reaction					1326:1333	quantitative polymerase chain reaction	1296:1333	quantitative polymerase chain reaction	1296:1333	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	4	43	theme	alkali-generating	720:736	arg1	bacteria					738:745	alkali-generating bacteria	720:745	alkali-generating bacteria	720:745	Here, we hypothesize that arginine may augment the ecological benefit of fluoride by enriching alkali-generating bacteria in the plaque biofilm and thus synergizes with fluoride in controlling dental caries.
25477312	10	44	theme	ecological	1988:1997	arg1	approach					1999:2006	a promising ecological approach	1976:2006	a promising ecological approach to caries management	1976:2027	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	6	45	theme	quantitative	1296:1307	arg1	reaction					1326:1333	quantitative polymerase chain reaction	1296:1333	quantitative polymerase chain reaction	1296:1333	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	5	46	theme	biofilm	917:923	arg1	cultures					925:932	planktonic and biofilm cultures	902:932	planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays	902:1050	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	10	47	theme	promising	1978:1986	arg1	approach					1999:2006	a promising ecological approach	1976:2006	a promising ecological approach to caries management	1976:2027	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	5	48	theme	gingivalis	1002:1011	arg1	cultures					925:932	planktonic and biofilm cultures	902:932	planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays	902:1050	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	7	49	theme	biofilm	1443:1449	arg1	cultures					1451:1458	both planktonic and biofilm cultures	1423:1458	cultures	1451:1458	We found that arginine synergized with fluoride in suppressing acidogenic S. mutans in both planktonic and biofilm cultures.
25477312	2	50	theme	used	262:265	arg1	agent					278:282	a widely used anticaries agent	253:282	a widely used anticaries agent	253:282	Fluoride is a widely used anticaries agent, which promotes tooth hard-tissue remineralization and suppresses bacterial activities.
25477312	2	50	theme	used	262:265	arg1	Fluoride					241:248	Fluoride	241:248	Fluoride	241:248	Fluoride is a widely used anticaries agent, which promotes tooth hard-tissue remineralization and suppresses bacterial activities.
25477312	6	51	theme	NaF/arginine	1065:1076	arg1	combinations					1078:1089	The optimal NaF/arginine combinations	1053:1089	The optimal NaF/arginine combinations	1053:1089	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	3	52	contain	containing	527:536	arg2	fluoride					538:545	fluoride	538:545	fluoride alone	538:551	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	3	52	contain	containing	527:536	arg1	those					521:525	those	521:525	those	521:525	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	9	53	theme	streptococcal	1669:1681	arg1	"					1691:1691	a "streptococcal pressure"	1666:1691	a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis	1666:1751	More importantly, the optimal combination of NaF/arginine maintained a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis within the alkalized biofilm.
25477312	5	54	theme	microdilution	1031:1043	arg1	assays					1045:1050	checkerboard microdilution assays	1018:1050	checkerboard microdilution assays	1018:1050	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	9	55	theme	NaF/arginine	1642:1653	arg1	combination					1627:1637	the optimal combination	1615:1637	the optimal combination of NaF/arginine	1615:1653	More importantly, the optimal combination of NaF/arginine maintained a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis within the alkalized biofilm.
25477312	1	56	theme	acidogenic/aciduric	143:161	arg1	pathogens					163:171	acidogenic/aciduric pathogens	143:171	acidogenic/aciduric pathogens	143:171	Dental caries is closely associated with the microbial disequilibrium between acidogenic/aciduric pathogens and alkali-generating commensal residents within the dental plaque.
25477312	1	57	theme	microbial	110:118	arg1	disequilibrium					120:133	the microbial disequilibrium	106:133	the microbial disequilibrium between acidogenic/aciduric pathogens and alkali-generating commensal residents within the dental plaque	106:238	Dental caries is closely associated with the microbial disequilibrium between acidogenic/aciduric pathogens and alkali-generating commensal residents within the dental plaque.
25477312	6	58	theme	microbial	1139:1147	arg1	composition					1149:1159	microbial composition	1139:1159	microbial composition	1139:1159	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	4	59	theme	plaque	754:759	arg1	biofilm					761:767	the plaque biofilm	750:767	the plaque biofilm	750:767	Here, we hypothesize that arginine may augment the ecological benefit of fluoride by enriching alkali-generating bacteria in the plaque biofilm and thus synergizes with fluoride in controlling dental caries.
25477312	5	60	theme	Porphyromonas	988:1000	arg1	gingivalis					1002:1011	Porphyromonas gingivalis	988:1011	Porphyromonas gingivalis	988:1011	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	3	61	theme	Recent	372:377	arg1	trials					388:393	Recent clinical trials	372:393	Recent clinical trials	372:393	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	0	62	from	effects	14:20	arg1	bacteria					55:62	oral bacteria	50:62	oral bacteria	50:62	Combinatorial effects of arginine and fluoride on oral bacteria.
25477312	8	63	theme	NaF/arginine	1478:1489	arg1	combination					1491:1501	the NaF/arginine combination	1474:1501	the NaF/arginine combination	1474:1501	In addition, the NaF/arginine combination synergistically reduced S. mutans but enriched S. sanguinis within the multispecies biofilms.
25477312	9	64	theme	oral	1725:1728	arg1	gingivalis					1742:1751	oral anaerobe P. gingivalis	1725:1751	oral anaerobe P. gingivalis	1725:1751	More importantly, the optimal combination of NaF/arginine maintained a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis within the alkalized biofilm.
25477312	3	65	theme	oral	411:414	arg1	products					424:431	oral hygiene products	411:431	oral hygiene products containing both fluoride and arginine	411:469	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	6	66	theme	species-specific	1240:1255	arg1	hybridization					1278:1290	bacterial species-specific fluorescence in situ hybridization	1230:1290	bacterial species-specific fluorescence in situ hybridization	1230:1290	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	9	67	theme	P.	1739:1740	arg1	gingivalis					1742:1751	oral anaerobe P. gingivalis	1725:1751	oral anaerobe P. gingivalis	1725:1751	More importantly, the optimal combination of NaF/arginine maintained a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis within the alkalized biofilm.
25477312	5	68	theme	NaF/arginine	886:897	arg1	effects					875:881	the combinatory effects	859:881	the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays	859:1050	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	6	69	theme	dual-	1195:1199	arg1	biofilm					1216:1222	single-, dual-, and 3-species biofilm	1186:1222	single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction	1186:1333	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	10	70	theme	combinatory	1820:1830	arg1	application					1832:1842	the combinatory application	1816:1842	the combinatory application of fluoride and arginine	1816:1867	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	0	71	theme	arginine	25:32	arg1	effects					14:20	Combinatorial effects	0:20	Combinatorial effects of arginine and fluoride on oral bacteria	0:62	Combinatorial effects of arginine and fluoride on oral bacteria.
25477312	9	72	theme	alkalized	1764:1772	arg1	biofilm					1774:1780	the alkalized biofilm	1760:1780	the alkalized biofilm	1760:1780	More importantly, the optimal combination of NaF/arginine maintained a "streptococcal pressure" against the potential growth of oral anaerobe P. gingivalis within the alkalized biofilm.
25477312	6	73	theme	single-	1186:1192	arg1	biofilm					1216:1222	single-, dual-, and 3-species biofilm	1186:1222	single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction	1186:1333	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	1	74	theme	Dental	65:70	arg1	caries					72:77	Dental caries	65:77	Dental caries	65:77	Dental caries is closely associated with the microbial disequilibrium between acidogenic/aciduric pathogens and alkali-generating commensal residents within the dental plaque.
25477312	0	75	theme	fluoride	38:45	arg1	effects					14:20	Combinatorial effects	0:20	Combinatorial effects of arginine and fluoride on oral bacteria	0:62	Combinatorial effects of arginine and fluoride on oral bacteria.
25477312	3	76	theme	anticaries	489:498	arg1	effect					500:505	a greater anticaries effect	479:505	a greater anticaries effect	479:505	Recent clinical trials have shown that oral hygiene products containing both fluoride and arginine possess a greater anticaries effect compared with those containing fluoride alone, indicating synergy between fluoride and arginine in caries management.
25477312	5	77	with	cultures	925:932	arg1	assays					1045:1050	checkerboard microdilution assays	1018:1050	checkerboard microdilution assays	1018:1050	Specifically, we assessed the combinatory effects of NaF/arginine on planktonic and biofilm cultures of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis with checkerboard microdilution assays.
25477312	0	78	theme	oral	50:53	arg1	bacteria					55:62	oral bacteria	50:62	oral bacteria	50:62	Combinatorial effects of arginine and fluoride on oral bacteria.
25477312	8	79	theme	multispecies	1574:1585	arg1	biofilms					1587:1594	the multispecies biofilms	1570:1594	the multispecies biofilms	1570:1594	In addition, the NaF/arginine combination synergistically reduced S. mutans but enriched S. sanguinis within the multispecies biofilms.
25477312	10	80	theme	caries	2011:2016	arg1	management					2018:2027	caries management	2011:2027	caries management	2011:2027	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	6	81	theme	fluorescence	1257:1268	arg1	hybridization					1278:1290	bacterial species-specific fluorescence in situ hybridization	1230:1290	bacterial species-specific fluorescence in situ hybridization	1230:1290	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	10	82	theme	potential	1875:1883	arg1	effect					1897:1902	a potential synergistic effect	1873:1902	a potential synergistic effect	1873:1902	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25477312	6	83	theme	bacterial	1230:1238	arg1	hybridization					1278:1290	bacterial species-specific fluorescence in situ hybridization	1230:1290	bacterial species-specific fluorescence in situ hybridization	1230:1290	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	6	84	dep	in	1270:1271	arg1	situ					1273:1276	situ	1273:1276	situ	1273:1276	The optimal NaF/arginine combinations were selected, and their combinatory effects on microbial composition were further examined in single-, dual-, and 3-species biofilm using bacterial species-specific fluorescence in situ hybridization and quantitative polymerase chain reaction.
25477312	10	85	theme	healthy	1921:1927	arg1	equilibrium					1944:1954	a healthy oral microbial equilibrium	1919:1954	a healthy oral microbial equilibrium	1919:1954	Taken together, we conclude that the combinatory application of fluoride and arginine has a potential synergistic effect in maintaining a healthy oral microbial equilibrium and thus represents a promising ecological approach to caries management.
25693888	10	0	theme	other	1761:1765	arg1	composites					1792:1801	other siRNA-conjugated AuNP-HA composites	1761:1801	other siRNA-conjugated AuNP-HA composites	1761:1801	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	9	1	theme	matrix	1413:1418	arg1	metalloproteinase-2					1420:1438	the secreted matrix metalloproteinase-2	1400:1438	the secreted matrix metalloproteinase-2	1400:1438	The BaP-elicited cell migration and enzymatic activity of the secreted matrix metalloproteinase-2 were also substantially suppressed by treatment with AuNP-HA-IAP-2 siRNAs.
25693888	3	2	theme	Functionalized	453:466	arg1	AuNP					487:490	AuNP	487:490	AuNP	487:490	Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials.
25693888	3	2	theme	Functionalized	453:466	arg1	nanoparticle					473:484	Functionalized gold nanoparticle	453:484	Functionalized gold nanoparticle (AuNP)	453:491	Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials.
25693888	3	2	theme	Functionalized	453:466	arg1	system					518:523	an effective delivery system	496:523	an effective delivery system for bio-active materials	496:548	Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials.
25693888	10	3	theme	lung	1818:1821	arg1	cancer					1823:1828	COF-induced lung cancer	1806:1828	COF-induced lung cancer	1806:1828	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	8	4	theme	cell	1176:1179	arg1	proliferation					1181:1193	cell proliferation	1176:1193	cell proliferation	1176:1193	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	6	5	theme	siRNA	850:854	arg1	Conjugation					829:839	Conjugation	829:839	Conjugation of IAP-2 siRNA into AuNPs-HA	829:868	Conjugation of IAP-2 siRNA into AuNPs-HA was verified by the UV-vis spectrometer and Fourier transform infrared spectrometer.
25693888	10	6	theme	A549	1591:1594	arg1	cells					1596:1600	A549 cells	1591:1600	A549 cells	1591:1600	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	0	7	theme	A549	160:163	arg1	cells					165:169	lung cancer A549 cells	148:169	lung cancer A549 cells	148:169	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	10	8	theme	gene-caused	1840:1850	arg1	diseases					1852:1859	other gene-caused diseases	1834:1859	other gene-caused diseases	1834:1859	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	8	9	theme	pronounced	1209:1218	arg1	apoptosis					1225:1233	pronounced cell apoptosis	1209:1233	pronounced cell apoptosis	1209:1233	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	4	10	theme	target	642:647	arg1	specificity					649:659	the target specificity	638:659	the target specificity	638:659	Here, biocompatible hyaluronic acid (HA) was fabricated into nanoparticles to increase the target specificity by binding to CD44-over-expressed cancer cells.
25693888	0	11	theme	lung	148:151	arg1	cells					165:169	lung cancer A549 cells	148:169	lung cancer A549 cells	148:169	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	1	12	theme	proteins	349:356	arg1	inhibitor					308:316	inhibitor	308:316	inhibitor of apoptosis protein-2 (IAP-2) proteins	308:356	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	10	13	theme	IAP-2	1643:1647	arg1	expression					1649:1658	the IAP-2 expression	1639:1658	the IAP-2 expression	1639:1658	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	7	14	theme	A549	1025:1028	arg1	cells					1030:1034	A549 cells	1025:1034	A549 cells	1025:1034	Further studies showed that AuNP-HA/FITC were effectively taken up by A549 cells through CD44-mediated endocytosis.
25693888	6	15	dep	Fourier	914:920	arg1	transform					922:930	transform	922:930	transform infrared spectrometer	922:952	Conjugation of IAP-2 siRNA into AuNPs-HA was verified by the UV-vis spectrometer and Fourier transform infrared spectrometer.
25693888	5	16	theme	IAP-2-specific	709:722	arg1	siRNAs					747:752	siRNAs	747:752	siRNAs	747:752	IAP-2-specific small-interfering RNA (siRNAs) or fluorescein isothiocyanate (FITC) were then incorporated into AuNP-HA.
25693888	5	16	theme	IAP-2-specific	709:722	arg1	RNA					742:744	IAP-2-specific small-interfering RNA	709:744	IAP-2-specific small-interfering RNA (siRNAs)	709:753	IAP-2-specific small-interfering RNA (siRNAs) or fluorescein isothiocyanate (FITC) were then incorporated into AuNP-HA.
25693888	0	17	theme	siRNAs	85:90	arg1	inhibitor					52:60	the inhibitor	48:60	the inhibitor of apoptosis protein-2 siRNAs	48:90	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	1	18	theme	Benzo[a	172:178	arg1	BaP					188:190	BaP	188:190	BaP	188:190	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	1	18	theme	Benzo[a	172:178	arg1	pyrene					180:185	Benzo[a]pyrene	172:185	Benzo[a]pyrene (BaP)	172:191	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	1	18	theme	Benzo[a	172:178	arg1	component					196:204	a component	194:204	a component of cooking oil fumes (COF)	194:231	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	8	19	theme	caspases-3	1330:1339	arg1	protein					1292:1298	Bax protein	1288:1298	Bax protein as well as the active form of caspases-3	1288:1339	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	8	19	theme	caspases-3	1330:1339	arg1	form					1322:1325	the active form	1311:1325	Bax protein as well as the active form of caspases-3	1288:1339	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	0	20	theme	pyrene-induced	109:122	arg1	properties					134:143	benzo[a]pyrene-induced oncogenic properties	101:143	benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells	101:169	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	1	21	theme	protein-2	331:339	arg1	proteins					349:356	apoptosis protein-2 (IAP-2) proteins	321:356	apoptosis protein-2 (IAP-2) proteins	321:356	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	2	22	theme	promising	394:402	arg1	knockdown					364:372	knockdown	364:372	knockdown of IAP-2	364:381	Thus knockdown of IAP-2 would be a promising way to battle against lung cancer caused by COF.
25693888	2	22	theme	promising	394:402	arg1	way					404:406	a promising way	392:406	a promising way to battle against lung cancer caused by COF	392:450	Thus knockdown of IAP-2 would be a promising way to battle against lung cancer caused by COF.
25693888	4	23	theme	CD44-over-expressed	675:693	arg1	cells					702:706	CD44-over-expressed cancer cells	675:706	CD44-over-expressed cancer cells	675:706	Here, biocompatible hyaluronic acid (HA) was fabricated into nanoparticles to increase the target specificity by binding to CD44-over-expressed cancer cells.
25693888	0	24	theme	Hyaluronic	0:9	arg1	delivery					36:43	Hyaluronic acid-fabricated nanogold delivery	0:43	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs	0:90	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	2	25	theme	IAP-2	377:381	arg1	knockdown					364:372	knockdown	364:372	knockdown of IAP-2	364:381	Thus knockdown of IAP-2 would be a promising way to battle against lung cancer caused by COF.
25693888	2	25	theme	IAP-2	377:381	arg1	way					404:406	a promising way	392:406	a promising way to battle against lung cancer caused by COF	392:450	Thus knockdown of IAP-2 would be a promising way to battle against lung cancer caused by COF.
25693888	0	26	theme	nanogold	27:34	arg1	delivery					36:43	Hyaluronic acid-fabricated nanogold delivery	0:43	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs	0:90	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	1	27	theme	cancer	248:253	arg1	proliferation					260:272	lung cancer cell proliferation	243:272	lung cancer cell proliferation	243:272	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	9	28	with	treatment	1478:1486	arg1	siRNAs					1507:1512	AuNP-HA-IAP-2 siRNAs	1493:1512	AuNP-HA-IAP-2 siRNAs	1493:1512	The BaP-elicited cell migration and enzymatic activity of the secreted matrix metalloproteinase-2 were also substantially suppressed by treatment with AuNP-HA-IAP-2 siRNAs.
25693888	9	29	theme	BaP-elicited	1346:1357	arg1	migration					1364:1372	The BaP-elicited cell migration	1342:1372	The BaP-elicited cell migration	1342:1372	The BaP-elicited cell migration and enzymatic activity of the secreted matrix metalloproteinase-2 were also substantially suppressed by treatment with AuNP-HA-IAP-2 siRNAs.
25693888	0	30	theme	apoptosis	65:73	arg1	protein-2					75:83	apoptosis protein-2	65:83	apoptosis protein-2 siRNAs	65:90	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	0	31	theme	inhibitor	52:60	arg1	delivery					36:43	Hyaluronic acid-fabricated nanogold delivery	0:43	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs	0:90	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	8	32	theme	cells	1100:1104	arg1	Incubation					1071:1080	Incubation	1071:1080	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs	1071:1130	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	10	33	theme	IAP-2	1544:1548	arg1	siRNAs					1550:1555	IAP-2 siRNAs	1544:1555	IAP-2 siRNAs	1544:1555	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	10	34	theme	IAP-2	1746:1750	arg1	siRNA					1752:1756	IAP-2 siRNA	1746:1756	IAP-2 siRNA	1746:1756	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	9	35	theme	enzymatic	1378:1386	arg1	activity					1388:1395	enzymatic activity	1378:1395	enzymatic activity	1378:1395	The BaP-elicited cell migration and enzymatic activity of the secreted matrix metalloproteinase-2 were also substantially suppressed by treatment with AuNP-HA-IAP-2 siRNAs.
25693888	8	36	theme	AuNP-HA-IAP-2	1111:1123	arg1	siRNAs					1125:1130	AuNP-HA-IAP-2 siRNAs	1111:1130	AuNP-HA-IAP-2 siRNAs	1111:1130	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	8	37	with	Incubation	1071:1080	arg1	siRNAs					1125:1130	AuNP-HA-IAP-2 siRNAs	1111:1130	AuNP-HA-IAP-2 siRNAs	1111:1130	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	10	38	theme	composites	1792:1801	arg1	application					1731:1741	the potential therapeutic application	1705:1741	the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases	1705:1859	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	1	39	theme	oil	217:219	arg1	COF					228:230	COF	228:230	COF	228:230	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	1	39	theme	oil	217:219	arg1	fumes					221:225	cooking oil fumes	209:225	cooking oil fumes (COF)	209:231	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	0	40	theme	acid-fabricated	11:25	arg1	delivery					36:43	Hyaluronic acid-fabricated nanogold delivery	0:43	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs	0:90	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	10	41	theme	siRNA-conjugated	1767:1782	arg1	composites					1792:1801	other siRNA-conjugated AuNP-HA composites	1761:1801	other siRNA-conjugated AuNP-HA composites	1761:1801	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	9	42	theme	secreted	1404:1411	arg1	metalloproteinase-2					1420:1438	the secreted matrix metalloproteinase-2	1400:1438	the secreted matrix metalloproteinase-2	1400:1438	The BaP-elicited cell migration and enzymatic activity of the secreted matrix metalloproteinase-2 were also substantially suppressed by treatment with AuNP-HA-IAP-2 siRNAs.
25693888	10	43	theme	functionalized	1605:1618	arg1	AuNP-HA					1620:1626	functionalized AuNP-HA	1605:1626	functionalized AuNP-HA	1605:1626	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	9	44	theme	metalloproteinase-2	1420:1438	arg1	migration					1364:1372	The BaP-elicited cell migration	1342:1372	The BaP-elicited cell migration	1342:1372	The BaP-elicited cell migration and enzymatic activity of the secreted matrix metalloproteinase-2 were also substantially suppressed by treatment with AuNP-HA-IAP-2 siRNAs.
25693888	9	44	theme	metalloproteinase-2	1420:1438	arg1	activity					1388:1395	enzymatic activity	1378:1395	enzymatic activity	1378:1395	The BaP-elicited cell migration and enzymatic activity of the secreted matrix metalloproteinase-2 were also substantially suppressed by treatment with AuNP-HA-IAP-2 siRNAs.
25693888	8	45	theme	IAP-2	1159:1163	arg1	expression					1145:1154	the expression	1141:1154	the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis	1141:1233	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	10	46	theme	COF-induced	1806:1816	arg1	cancer					1823:1828	COF-induced lung cancer	1806:1828	COF-induced lung cancer	1806:1828	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	6	47	theme	IAP-2	844:848	arg1	siRNA					850:854	IAP-2 siRNA	844:854	IAP-2 siRNA	844:854	Conjugation of IAP-2 siRNA into AuNPs-HA was verified by the UV-vis spectrometer and Fourier transform infrared spectrometer.
25693888	3	48	theme	effective	499:507	arg1	system					518:523	an effective delivery system	496:523	an effective delivery system for bio-active materials	496:548	Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials.
25693888	3	48	theme	effective	499:507	arg1	nanoparticle					473:484	Functionalized gold nanoparticle	453:484	Functionalized gold nanoparticle (AuNP)	453:491	Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials.
25693888	8	49	theme	Bcl-2	1254:1258	arg1	protein					1260:1266	Bcl-2 protein	1254:1266	Bcl-2 protein	1254:1266	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	7	50	theme	CD44-mediated	1044:1056	arg1	endocytosis					1058:1068	CD44-mediated endocytosis	1044:1068	CD44-mediated endocytosis	1044:1068	Further studies showed that AuNP-HA/FITC were effectively taken up by A549 cells through CD44-mediated endocytosis.
25693888	10	51	theme	other	1834:1838	arg1	diseases					1852:1859	other gene-caused diseases	1834:1859	other gene-caused diseases	1834:1859	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	3	52	theme	delivery	509:516	arg1	system					518:523	an effective delivery system	496:523	an effective delivery system for bio-active materials	496:548	Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials.
25693888	3	52	theme	delivery	509:516	arg1	nanoparticle					473:484	Functionalized gold nanoparticle	453:484	Functionalized gold nanoparticle (AuNP)	453:491	Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials.
25693888	4	53	theme	hyaluronic	571:580	arg1	HA					588:589	HA	588:589	HA	588:589	Here, biocompatible hyaluronic acid (HA) was fabricated into nanoparticles to increase the target specificity by binding to CD44-over-expressed cancer cells.
25693888	4	53	theme	hyaluronic	571:580	arg1	acid					582:585	biocompatible hyaluronic acid	557:585	biocompatible hyaluronic acid (HA)	557:590	Here, biocompatible hyaluronic acid (HA) was fabricated into nanoparticles to increase the target specificity by binding to CD44-over-expressed cancer cells.
25693888	10	54	theme	BaP-induced	1664:1674	arg1	events					1686:1691	BaP-induced oncogenic events	1664:1691	BaP-induced oncogenic events	1664:1691	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	0	55	theme	cancer	153:158	arg1	cells					165:169	lung cancer A549 cells	148:169	lung cancer A549 cells	148:169	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	3	56	theme	bio-active	529:538	arg1	materials					540:548	bio-active materials	529:548	bio-active materials	529:548	Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials.
25693888	9	57	theme	AuNP-HA-IAP-2	1493:1505	arg1	siRNAs					1507:1512	AuNP-HA-IAP-2 siRNAs	1493:1512	AuNP-HA-IAP-2 siRNAs	1493:1512	The BaP-elicited cell migration and enzymatic activity of the secreted matrix metalloproteinase-2 were also substantially suppressed by treatment with AuNP-HA-IAP-2 siRNAs.
25693888	8	58	theme	cell	1220:1223	arg1	apoptosis					1225:1233	pronounced cell apoptosis	1209:1233	pronounced cell apoptosis	1209:1233	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	0	59	theme	cells	165:169	arg1	properties					134:143	benzo[a]pyrene-induced oncogenic properties	101:143	benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells	101:169	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	6	60	theme	UV-vis	890:895	arg1	spectrometer					897:908	the UV-vis spectrometer	886:908	the UV-vis spectrometer	886:908	Conjugation of IAP-2 siRNA into AuNPs-HA was verified by the UV-vis spectrometer and Fourier transform infrared spectrometer.
25693888	6	61	dep	transform	922:930	arg1	infrared					932:939	infrared	932:939	transform infrared spectrometer	922:952	Conjugation of IAP-2 siRNA into AuNPs-HA was verified by the UV-vis spectrometer and Fourier transform infrared spectrometer.
25693888	8	62	theme	Bax	1288:1290	arg1	protein					1292:1298	Bax protein	1288:1298	Bax protein as well as the active form of caspases-3	1288:1339	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	8	63	from	decrease	1242:1249	arg1	protein					1260:1266	Bcl-2 protein	1254:1266	Bcl-2 protein	1254:1266	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	8	63	from	decrease	1242:1249	arg1	protein					1292:1298	Bax protein	1288:1298	Bax protein as well as the active form of caspases-3	1288:1339	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	8	63	from	decrease	1242:1249	arg1	form					1322:1325	the active form	1311:1325	Bax protein as well as the active form of caspases-3	1288:1339	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	0	64	theme	protein-2	75:83	arg1	siRNAs					85:90	apoptosis protein-2 siRNAs	65:90	apoptosis protein-2 siRNAs	65:90	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	8	65	from	increase	1276:1283	arg1	protein					1260:1266	Bcl-2 protein	1254:1266	Bcl-2 protein	1254:1266	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	8	65	from	increase	1276:1283	arg1	protein					1292:1298	Bax protein	1288:1298	Bax protein as well as the active form of caspases-3	1288:1339	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	8	65	from	increase	1276:1283	arg1	form					1322:1325	the active form	1311:1325	Bax protein as well as the active form of caspases-3	1288:1339	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	5	66	theme	small-interfering	724:740	arg1	siRNAs					747:752	siRNAs	747:752	siRNAs	747:752	IAP-2-specific small-interfering RNA (siRNAs) or fluorescein isothiocyanate (FITC) were then incorporated into AuNP-HA.
25693888	5	66	theme	small-interfering	724:740	arg1	RNA					742:744	IAP-2-specific small-interfering RNA	709:744	IAP-2-specific small-interfering RNA (siRNAs)	709:753	IAP-2-specific small-interfering RNA (siRNAs) or fluorescein isothiocyanate (FITC) were then incorporated into AuNP-HA.
25693888	7	67	theme	Further	955:961	arg1	studies					963:969	Further studies	955:969	Further studies	955:969	Further studies showed that AuNP-HA/FITC were effectively taken up by A549 cells through CD44-mediated endocytosis.
25693888	2	68	theme	lung	426:429	arg1	cancer					431:436	lung cancer	426:436	lung cancer caused by COF	426:450	Thus knockdown of IAP-2 would be a promising way to battle against lung cancer caused by COF.
25693888	8	69	theme	active	1315:1320	arg1	form					1322:1325	the active form	1311:1325	Bax protein as well as the active form of caspases-3	1288:1339	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	1	70	theme	inhibitor	308:316	arg1	induction					295:303	the induction	291:303	the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins	291:356	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	8	71	dep	IAP-2	1159:1163	arg1	triggered					1199:1207	triggered	1199:1207	triggered pronounced cell apoptosis	1199:1233	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	8	71	dep	IAP-2	1159:1163	arg1	decreased					1166:1174	decreased	1166:1174	decreased cell proliferation	1166:1193	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	0	72	theme	oncogenic	124:132	arg1	properties					134:143	benzo[a]pyrene-induced oncogenic properties	101:143	benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells	101:169	Hyaluronic acid-fabricated nanogold delivery of the inhibitor of apoptosis protein-2 siRNAs inhibits benzo[a]pyrene-induced oncogenic properties of lung cancer A549 cells.
25693888	1	73	theme	apoptosis	321:329	arg1	IAP-2					342:346	IAP-2	342:346	IAP-2	342:346	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	1	73	theme	apoptosis	321:329	arg1	protein-2					331:339	apoptosis protein-2	321:339	apoptosis protein-2 (IAP-2) proteins	321:356	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	5	74	theme	fluorescein	758:768	arg1	isothiocyanate					770:783	fluorescein isothiocyanate	758:783	fluorescein isothiocyanate (FITC)	758:790	IAP-2-specific small-interfering RNA (siRNAs) or fluorescein isothiocyanate (FITC) were then incorporated into AuNP-HA.
25693888	5	74	theme	fluorescein	758:768	arg1	FITC					786:789	FITC	786:789	FITC	786:789	IAP-2-specific small-interfering RNA (siRNAs) or fluorescein isothiocyanate (FITC) were then incorporated into AuNP-HA.
25693888	10	75	theme	oncogenic	1676:1684	arg1	events					1686:1691	BaP-induced oncogenic events	1664:1691	BaP-induced oncogenic events	1664:1691	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	1	76	theme	lung	243:246	arg1	proliferation					260:272	lung cancer cell proliferation	243:272	lung cancer cell proliferation	243:272	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	4	77	theme	cancer	695:700	arg1	cells					702:706	CD44-over-expressed cancer cells	675:706	CD44-over-expressed cancer cells	675:706	Here, biocompatible hyaluronic acid (HA) was fabricated into nanoparticles to increase the target specificity by binding to CD44-over-expressed cancer cells.
25693888	10	78	theme	therapeutic	1719:1729	arg1	application					1731:1741	the potential therapeutic application	1705:1741	the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases	1705:1859	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	1	79	theme	cell	255:258	arg1	proliferation					260:272	lung cancer cell proliferation	243:272	lung cancer cell proliferation	243:272	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	8	80	theme	BaP-challenged	1085:1098	arg1	cells					1100:1104	BaP-challenged cells	1085:1104	BaP-challenged cells	1085:1104	Incubation of BaP-challenged cells with AuNP-HA-IAP-2 siRNAs silenced the expression of IAP-2, decreased cell proliferation and triggered pronounced cell apoptosis by the decrease in Bcl-2 protein and the increase in Bax protein as well as the active form of caspases-3.
25693888	9	81	theme	cell	1359:1362	arg1	migration					1364:1372	The BaP-elicited cell migration	1342:1372	The BaP-elicited cell migration	1342:1372	The BaP-elicited cell migration and enzymatic activity of the secreted matrix metalloproteinase-2 were also substantially suppressed by treatment with AuNP-HA-IAP-2 siRNAs.
25693888	10	82	theme	siRNA	1752:1756	arg1	application					1731:1741	the potential therapeutic application	1705:1741	the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases	1705:1859	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	3	83	theme	gold	468:471	arg1	AuNP					487:490	AuNP	487:490	AuNP	487:490	Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials.
25693888	3	83	theme	gold	468:471	arg1	nanoparticle					473:484	Functionalized gold nanoparticle	453:484	Functionalized gold nanoparticle (AuNP)	453:491	Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials.
25693888	3	83	theme	gold	468:471	arg1	system					518:523	an effective delivery system	496:523	an effective delivery system for bio-active materials	496:548	Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials.
25693888	1	84	theme	cooking	209:215	arg1	COF					228:230	COF	228:230	COF	228:230	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	1	84	theme	cooking	209:215	arg1	fumes					221:225	cooking oil fumes	209:225	cooking oil fumes (COF)	209:231	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	4	85	theme	biocompatible	557:569	arg1	HA					588:589	HA	588:589	HA	588:589	Here, biocompatible hyaluronic acid (HA) was fabricated into nanoparticles to increase the target specificity by binding to CD44-over-expressed cancer cells.
25693888	4	85	theme	biocompatible	557:569	arg1	acid					582:585	biocompatible hyaluronic acid	557:585	biocompatible hyaluronic acid (HA)	557:590	Here, biocompatible hyaluronic acid (HA) was fabricated into nanoparticles to increase the target specificity by binding to CD44-over-expressed cancer cells.
25693888	10	86	theme	AuNP-HA	1784:1790	arg1	composites					1792:1801	other siRNA-conjugated AuNP-HA composites	1761:1801	other siRNA-conjugated AuNP-HA composites	1761:1801	These results indicated that IAP-2 siRNAs can be efficiently delivered into A549 cells by functionalized AuNP-HA to repress the IAP-2 expression and BaP-induced oncogenic events, suggesting the potential therapeutic application of IAP-2 siRNA or other siRNA-conjugated AuNP-HA composites to COF-induced lung cancer and other gene-caused diseases in the future.
25693888	1	87	theme	fumes	221:225	arg1	pyrene					180:185	Benzo[a]pyrene	172:185	Benzo[a]pyrene (BaP)	172:191	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
25693888	1	87	theme	fumes	221:225	arg1	component					196:204	a component	194:204	a component of cooking oil fumes (COF)	194:231	Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction of inhibitor of apoptosis protein-2 (IAP-2) proteins.
28614348	7	0	theme	continental	1230:1240	arg1	crust					1250:1254	the continental Archean crust	1226:1254	the continental Archean crust	1226:1254	Obviously, the liquid phase in the continental Archean crust provided an interesting choice of functional organic molecules.
28614348	6	1	located	found	1095:1099	arg1	inclusions					1108:1117	the inclusions	1104:1117	the inclusions	1104:1117	Stable-isotope analysis confirms that the methane found in the inclusions has most likely been formed from abiotic sources by hydrothermal chemistry.
28614348	6	1	located	found	1095:1099	arg2	methane					1087:1093	the methane	1083:1093	the methane found in the inclusions	1083:1117	Stable-isotope analysis confirms that the methane found in the inclusions has most likely been formed from abiotic sources by hydrothermal chemistry.
28614348	1	2	theme	unsolved	136:143	arg1	origin					109:114	The origin	105:114	The origin of life	105:122	The origin of life is still an unsolved mystery in science.
28614348	1	2	theme	unsolved	136:143	arg1	mystery					145:151	an unsolved mystery	133:151	an unsolved mystery in science	133:162	The origin of life is still an unsolved mystery in science.
28614348	0	3	theme	chemistry	79:87	arg1	quartz-Analogues					49:64	Archean quartz-Analogues	41:64	Archean quartz-Analogues of prebiotic chemistry on early Earth	41:102	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.
28614348	0	4	from	compounds	8:16	arg1	inclusions					27:36	fluid inclusions	21:36	fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth	21:102	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.
28614348	2	5	theme	prebiotic	181:189	arg1	chemistry					191:199	prebiotic chemistry	181:199	prebiotic chemistry	181:199	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	3	6	theme	quartz	499:504	arg1	crystals					506:513	quartz crystals	499:513	quartz crystals which have grown in this environment during the Archean period	499:576	The composition of fluid inclusions in minerals such as quartz crystals which have grown in this environment during the Archean period might provide important information about the first organic molecules formed by hydrothermal synthesis.
28614348	1	7	from	mystery	145:151	arg1	science					156:162	science	156:162	science	156:162	The origin of life is still an unsolved mystery in science.
28614348	4	8	theme	Archean	773:779	arg1	minerals					788:795	Archean quartz minerals	773:795	Archean quartz minerals from Western Australia	773:818	Here we present evidence for organic compounds which were preserved in fluid inclusions of Archean quartz minerals from Western Australia.
28614348	4	9	theme	fluid	753:757	arg1	inclusions					759:768	fluid inclusions	753:768	fluid inclusions of Archean quartz minerals from Western Australia	753:818	Here we present evidence for organic compounds which were preserved in fluid inclusions of Archean quartz minerals from Western Australia.
28614348	0	10	theme	early	92:96	arg1	Earth					98:102	early Earth	92:102	early Earth	92:102	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.
28614348	0	11	from	quartz-Analogues	49:64	arg1	Earth					98:102	early Earth	92:102	early Earth	92:102	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.
28614348	7	12	theme	interesting	1268:1278	arg1	choice					1280:1285	an interesting choice	1265:1285	an interesting choice of functional organic molecules	1265:1317	Obviously, the liquid phase in the continental Archean crust provided an interesting choice of functional organic molecules.
28614348	5	13	theme	prebiotic	973:981	arg1	molecules					991:999	simple and even more complex prebiotic organic molecules	944:999	simple and even more complex prebiotic organic molecules	944:999	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	7	14	theme	liquid	1210:1215	arg1	phase					1217:1221	the liquid phase	1206:1221	the liquid phase in the continental Archean crust	1206:1254	Obviously, the liquid phase in the continental Archean crust provided an interesting choice of functional organic molecules.
28614348	7	15	theme	molecules	1309:1317	arg1	choice					1280:1285	an interesting choice	1265:1285	an interesting choice of functional organic molecules	1265:1317	Obviously, the liquid phase in the continental Archean crust provided an interesting choice of functional organic molecules.
28614348	5	16	theme	organic	843:849	arg1	compounds					851:859	organic compounds	843:859	organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes	843:1042	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	5	16	theme	organic	843:849	arg1	alkanes					869:875	alkanes	869:875	alkanes	869:875	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	5	16	theme	organic	843:849	arg1	aldehydes					904:912	aldehydes	904:912	aldehydes	904:912	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	5	16	theme	organic	843:849	arg1	alcohols					891:898	alcohols	891:898	alcohols	891:898	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	5	16	theme	organic	843:849	arg1	halocarbons					878:888	halocarbons	878:888	halocarbons	878:888	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	2	17	theme	hydrothermal	257:268	arg1	environment					270:280	the hydrothermal environment	253:280	the hydrothermal environment of tectonic fault zones	253:304	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	8	18	theme	cells	1494:1498	arg1	formation					1474:1482	the formation	1470:1482	the formation of living cells	1470:1498	We conclude that organic substances such as these could have made an important contribution to prebiotic chemistry which might eventually have led to the formation of living cells.
28614348	4	19	from	inclusions	759:768	arg1	Australia					810:818	Western Australia	802:818	Western Australia	802:818	Here we present evidence for organic compounds which were preserved in fluid inclusions of Archean quartz minerals from Western Australia.
28614348	4	20	theme	quartz	781:786	arg1	minerals					788:795	Archean quartz minerals	773:795	Archean quartz minerals from Western Australia	773:818	Here we present evidence for organic compounds which were preserved in fluid inclusions of Archean quartz minerals from Western Australia.
28614348	0	21	from	inclusions	27:36	arg1	Earth					98:102	early Earth	92:102	early Earth	92:102	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.
28614348	7	22	theme	Archean	1242:1248	arg1	crust					1250:1254	the continental Archean crust	1226:1254	the continental Archean crust	1226:1254	Obviously, the liquid phase in the continental Archean crust provided an interesting choice of functional organic molecules.
28614348	3	23	theme	hydrothermal	658:669	arg1	synthesis					671:679	hydrothermal synthesis	658:679	hydrothermal synthesis	658:679	The composition of fluid inclusions in minerals such as quartz crystals which have grown in this environment during the Archean period might provide important information about the first organic molecules formed by hydrothermal synthesis.
28614348	5	24	theme	simple	944:949	arg1	molecules					991:999	simple and even more complex prebiotic organic molecules	944:999	simple and even more complex prebiotic organic molecules	944:999	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	0	25	theme	Organic	0:6	arg1	compounds					8:16	Organic compounds	0:16	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.	0:103	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.
28614348	2	26	theme	zones	300:304	arg1	environment					270:280	the hydrothermal environment	253:280	the hydrothermal environment of tectonic fault zones	253:304	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	2	27	theme	sensitive	359:367	arg1	molecules					369:377	sensitive molecules	359:377	sensitive molecules	359:377	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	0	28	theme	fluid	21:25	arg1	inclusions					27:36	fluid inclusions	21:36	fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth	21:102	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.
28614348	8	29	theme	organic	1337:1343	arg1	substances					1345:1354	organic substances	1337:1354	organic substances such as these	1337:1368	We conclude that organic substances such as these could have made an important contribution to prebiotic chemistry which might eventually have led to the formation of living cells.
28614348	8	29	theme	organic	1337:1343	arg1	these					1364:1368	these	1364:1368	these	1364:1368	We conclude that organic substances such as these could have made an important contribution to prebiotic chemistry which might eventually have led to the formation of living cells.
28614348	2	30	theme	fault	294:298	arg1	zones					300:304	tectonic fault zones	285:304	tectonic fault zones	285:304	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	5	31	theme	hydrothermal	1021:1032	arg1	processes					1034:1042	hydrothermal processes	1021:1042	hydrothermal processes	1021:1042	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	5	32	theme	complex	965:971	arg1	molecules					991:999	simple and even more complex prebiotic organic molecules	944:999	simple and even more complex prebiotic organic molecules	944:999	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	3	33	theme	Archean	563:569	arg1	period					571:576	the Archean period	559:576	the Archean period	559:576	The composition of fluid inclusions in minerals such as quartz crystals which have grown in this environment during the Archean period might provide important information about the first organic molecules formed by hydrothermal synthesis.
28614348	4	34	theme	minerals	788:795	arg1	inclusions					759:768	fluid inclusions	753:768	fluid inclusions of Archean quartz minerals from Western Australia	753:818	Here we present evidence for organic compounds which were preserved in fluid inclusions of Archean quartz minerals from Western Australia.
28614348	2	35	theme	tectonic	285:292	arg1	zones					300:304	tectonic fault zones	285:304	tectonic fault zones	285:304	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	3	36	theme	fluid	462:466	arg1	inclusions					468:477	fluid inclusions	462:477	fluid inclusions	462:477	The composition of fluid inclusions in minerals such as quartz crystals which have grown in this environment during the Archean period might provide important information about the first organic molecules formed by hydrothermal synthesis.
28614348	4	37	theme	organic	711:717	arg1	compounds					719:727	organic compounds	711:727	organic compounds which were preserved in fluid inclusions of Archean quartz minerals from Western Australia	711:818	Here we present evidence for organic compounds which were preserved in fluid inclusions of Archean quartz minerals from Western Australia.
28614348	7	38	from	phase	1217:1221	arg1	crust					1250:1254	the continental Archean crust	1226:1254	the continental Archean crust	1226:1254	Obviously, the liquid phase in the continental Archean crust provided an interesting choice of functional organic molecules.
28614348	2	39	theme	protocells	222:231	arg1	chemistry					191:199	prebiotic chemistry	181:199	prebiotic chemistry	181:199	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	2	39	theme	protocells	222:231	arg1	formation					209:217	the formation	205:217	the formation of protocells	205:231	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	5	40	theme	compounds	851:859	arg1	compounds					851:859	organic compounds	843:859	organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes	843:1042	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	5	40	theme	compounds	851:859	arg1	alcohols					891:898	alcohols	891:898	alcohols	891:898	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	5	40	theme	compounds	851:859	arg1	aldehydes					904:912	aldehydes	904:912	aldehydes	904:912	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	5	40	theme	compounds	851:859	arg1	variety					832:838	a variety	830:838	a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes	830:1042	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	5	40	theme	compounds	851:859	arg1	alkanes					869:875	alkanes	869:875	alkanes	869:875	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	5	40	theme	compounds	851:859	arg1	halocarbons					878:888	halocarbons	878:888	halocarbons	878:888	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	3	41	theme	organic	630:636	arg1	molecules					638:646	the first organic molecules	620:646	the first organic molecules formed by hydrothermal synthesis	620:679	The composition of fluid inclusions in minerals such as quartz crystals which have grown in this environment during the Archean period might provide important information about the first organic molecules formed by hydrothermal synthesis.
28614348	2	42	theme	UV	429:430	arg1	radiation					432:440	UV radiation	429:440	UV radiation	429:440	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	0	43	theme	quartz-Analogues	49:64	arg1	inclusions					27:36	fluid inclusions	21:36	fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth	21:102	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.
28614348	8	44	theme	living	1487:1492	arg1	cells					1494:1498	living cells	1487:1498	living cells	1487:1498	We conclude that organic substances such as these could have made an important contribution to prebiotic chemistry which might eventually have led to the formation of living cells.
28614348	3	45	from	composition	447:457	arg1	minerals					482:489	minerals	482:489	minerals such as quartz crystals which have grown in this environment during the Archean period	482:576	The composition of fluid inclusions in minerals such as quartz crystals which have grown in this environment during the Archean period might provide important information about the first organic molecules formed by hydrothermal synthesis.
28614348	3	45	from	composition	447:457	arg1	crystals					506:513	quartz crystals	499:513	quartz crystals which have grown in this environment during the Archean period	499:576	The composition of fluid inclusions in minerals such as quartz crystals which have grown in this environment during the Archean period might provide important information about the first organic molecules formed by hydrothermal synthesis.
28614348	6	46	theme	abiotic	1152:1158	arg1	sources					1160:1166	abiotic sources	1152:1166	abiotic sources	1152:1166	Stable-isotope analysis confirms that the methane found in the inclusions has most likely been formed from abiotic sources by hydrothermal chemistry.
28614348	0	47	theme	Archean	41:47	arg1	quartz-Analogues					49:64	Archean quartz-Analogues	41:64	Archean quartz-Analogues of prebiotic chemistry on early Earth	41:102	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.
28614348	3	48	theme	inclusions	468:477	arg1	composition					447:457	The composition	443:457	The composition of fluid inclusions in minerals such as quartz crystals which have grown in this environment during the Archean period	443:576	The composition of fluid inclusions in minerals such as quartz crystals which have grown in this environment during the Archean period might provide important information about the first organic molecules formed by hydrothermal synthesis.
28614348	2	49	theme	continental	319:329	arg1	crust					331:335	the upper continental crust	309:335	the upper continental crust	309:335	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	2	49	theme	continental	319:329	arg1	environment					341:351	an environment	338:351	an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation	338:440	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	4	50	theme	Western	802:808	arg1	Australia					810:818	Western Australia	802:818	Western Australia	802:818	Here we present evidence for organic compounds which were preserved in fluid inclusions of Archean quartz minerals from Western Australia.
28614348	3	51	theme	important	592:600	arg1	information					602:612	important information	592:612	important information about the first organic molecules formed by hydrothermal synthesis	592:679	The composition of fluid inclusions in minerals such as quartz crystals which have grown in this environment during the Archean period might provide important information about the first organic molecules formed by hydrothermal synthesis.
28614348	1	52	theme	life	119:122	arg1	origin					109:114	The origin	105:114	The origin of life	105:122	The origin of life is still an unsolved mystery in science.
28614348	1	52	theme	life	119:122	arg1	mystery					145:151	an unsolved mystery	133:151	an unsolved mystery in science	133:162	The origin of life is still an unsolved mystery in science.
28614348	8	53	theme	prebiotic	1415:1423	arg1	chemistry					1425:1433	prebiotic chemistry	1415:1433	prebiotic chemistry	1415:1433	We conclude that organic substances such as these could have made an important contribution to prebiotic chemistry which might eventually have led to the formation of living cells.
28614348	6	54	theme	Stable-isotope	1045:1058	arg1	analysis					1060:1067	Stable-isotope analysis	1045:1067	Stable-isotope analysis	1045:1067	Stable-isotope analysis confirms that the methane found in the inclusions has most likely been formed from abiotic sources by hydrothermal chemistry.
28614348	0	55	from	Earth	98:102	arg1	inclusions					27:36	fluid inclusions	21:36	fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth	21:102	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.
28614348	3	56	theme	first	624:628	arg1	molecules					638:646	the first organic molecules	620:646	the first organic molecules formed by hydrothermal synthesis	620:679	The composition of fluid inclusions in minerals such as quartz crystals which have grown in this environment during the Archean period might provide important information about the first organic molecules formed by hydrothermal synthesis.
28614348	7	57	theme	organic	1301:1307	arg1	molecules					1309:1317	functional organic molecules	1290:1317	functional organic molecules	1290:1317	Obviously, the liquid phase in the continental Archean crust provided an interesting choice of functional organic molecules.
28614348	8	58	theme	important	1389:1397	arg1	contribution					1399:1410	an important contribution	1386:1410	an important contribution to prebiotic chemistry which might eventually have led to the formation of living cells	1386:1498	We conclude that organic substances such as these could have made an important contribution to prebiotic chemistry which might eventually have led to the formation of living cells.
28614348	2	59	theme	upper	313:317	arg1	crust					331:335	the upper continental crust	309:335	the upper continental crust	309:335	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	2	59	theme	upper	313:317	arg1	environment					341:351	an environment	338:351	an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation	338:440	Hypothetically, prebiotic chemistry and the formation of protocells may have evolved in the hydrothermal environment of tectonic fault zones in the upper continental crust, an environment where sensitive molecules are protected against degradation induced e.g. by UV radiation.
28614348	0	60	theme	prebiotic	69:77	arg1	chemistry					79:87	prebiotic chemistry	69:87	prebiotic chemistry	69:87	Organic compounds in fluid inclusions of Archean quartz-Analogues of prebiotic chemistry on early Earth.
28614348	5	61	theme	organic	983:989	arg1	molecules					991:999	simple and even more complex prebiotic organic molecules	944:999	simple and even more complex prebiotic organic molecules	944:999	We found a variety of organic compounds such as alkanes, halocarbons, alcohols and aldehydes which unambiguously show that simple and even more complex prebiotic organic molecules have been formed by hydrothermal processes.
28614348	4	62	from	Australia	810:818	arg1	inclusions					759:768	fluid inclusions	753:768	fluid inclusions of Archean quartz minerals from Western Australia	753:818	Here we present evidence for organic compounds which were preserved in fluid inclusions of Archean quartz minerals from Western Australia.
28614348	4	62	from	Australia	810:818	arg1	minerals					788:795	Archean quartz minerals	773:795	Archean quartz minerals from Western Australia	773:818	Here we present evidence for organic compounds which were preserved in fluid inclusions of Archean quartz minerals from Western Australia.
28614348	6	63	theme	hydrothermal	1171:1182	arg1	chemistry					1184:1192	hydrothermal chemistry	1171:1192	hydrothermal chemistry	1171:1192	Stable-isotope analysis confirms that the methane found in the inclusions has most likely been formed from abiotic sources by hydrothermal chemistry.
28614348	7	64	theme	functional	1290:1299	arg1	molecules					1309:1317	functional organic molecules	1290:1317	functional organic molecules	1290:1317	Obviously, the liquid phase in the continental Archean crust provided an interesting choice of functional organic molecules.
25260606	0	0	theme	thermally	81:89	arg1	gelation					118:125	thermally and photo-triggered tandem gelation	81:125	thermally and photo-triggered tandem gelation	81:125	A versatile bioink for three-dimensional printing of cellular scaffolds based on thermally and photo-triggered tandem gelation.
25260606	7	1	theme	HA-pNIPAAM	1033:1042	arg1	Elution					1022:1028	Elution	1022:1028	Elution of HA-pNIPAAM from the scaffold	1022:1060	Elution of HA-pNIPAAM from the scaffold was necessary to obtain good viability.
25260606	4	2	theme	grafted	592:598	arg1	HA-pNIPAAM					612:621	HA-pNIPAAM	612:621	HA-pNIPAAM	612:621	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	4	2	theme	grafted	592:598	arg1	hyaluronan					600:609	the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan	535:609	the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM)	535:622	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	5	3	theme	fast	742:745	arg1	gelation					747:754	fast gelation	742:754	fast gelation	742:754	HA-pNIPAAM provided fast gelation and immediate post-printing structural fidelity, while HAMA ensured long-term mechanical stability upon photocrosslinking.
25260606	6	4	theme	encapsulated	988:999	arg1	chondrocytes					1008:1019	encapsulated bovine chondrocytes	988:1019	encapsulated bovine chondrocytes	988:1019	The bioink was evaluated for rheological properties, swelling behavior, printability and biocompatibility of encapsulated bovine chondrocytes.
25260606	2	5	with	bioinks	307:313	arg1	properties					363:372	appropriate rheological and cytocompatible properties	320:372	appropriate rheological and cytocompatible properties	320:372	Printing of viable organ replacements, however, is dependent on bioinks with appropriate rheological and cytocompatible properties.
25260606	0	6	theme	photo-triggered	95:109	arg1	gelation					118:125	thermally and photo-triggered tandem gelation	81:125	thermally and photo-triggered tandem gelation	81:125	A versatile bioink for three-dimensional printing of cellular scaffolds based on thermally and photo-triggered tandem gelation.
25260606	4	7	theme	high-resolution	662:676	arg1	scaffolds					678:686	high-resolution scaffolds	662:686	high-resolution scaffolds with good viability	662:706	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	5	8	theme	mechanical	834:843	arg1	stability					845:853	long-term mechanical stability	824:853	long-term mechanical stability	824:853	HA-pNIPAAM provided fast gelation and immediate post-printing structural fidelity, while HAMA ensured long-term mechanical stability upon photocrosslinking.
25260606	4	9	theme	thermoresponsive	539:554	arg1	N-isopropylacrylamide					569:589	N-isopropylacrylamide	569:589	N-isopropylacrylamide	569:589	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	4	9	theme	thermoresponsive	539:554	arg1	poly					564:567	the thermoresponsive polymer poly	535:567	the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM)	535:622	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	8	10	theme	biopolymers	1170:1180	arg1	range					1161:1165	a range	1159:1165	a range of biopolymers which undergo tandem gelation, thereby facilitating the printing of cell-laden, stratified cartilage constructs with zonally varying composition and stiffness	1159:1339	HA-pNIPAAM can therefore be used to support extrusion of a range of biopolymers which undergo tandem gelation, thereby facilitating the printing of cell-laden, stratified cartilage constructs with zonally varying composition and stiffness.
25260606	8	11	theme	cell-laden	1250:1259	arg1	constructs					1283:1292	cell-laden, stratified cartilage constructs	1250:1292	cell-laden, stratified cartilage constructs	1250:1292	HA-pNIPAAM can therefore be used to support extrusion of a range of biopolymers which undergo tandem gelation, thereby facilitating the printing of cell-laden, stratified cartilage constructs with zonally varying composition and stiffness.
25260606	8	12	used	used	1130:1133	arg2	HA-pNIPAAM					1102:1111	HA-pNIPAAM	1102:1111	HA-pNIPAAM	1102:1111	HA-pNIPAAM can therefore be used to support extrusion of a range of biopolymers which undergo tandem gelation, thereby facilitating the printing of cell-laden, stratified cartilage constructs with zonally varying composition and stiffness.
25260606	8	13	theme	tandem	1196:1201	arg1	gelation					1203:1210	tandem gelation	1196:1210	tandem gelation	1196:1210	HA-pNIPAAM can therefore be used to support extrusion of a range of biopolymers which undergo tandem gelation, thereby facilitating the printing of cell-laden, stratified cartilage constructs with zonally varying composition and stiffness.
25260606	3	14	theme	poor	497:500	arg1	properties					511:520	generally poor printing properties	487:520	generally poor printing properties	487:520	In cartilage engineering, photocrosslinkable glycosaminoglycan-based hydrogels are chondrogenic, but alone have generally poor printing properties.
25260606	0	15	theme	tandem	111:116	arg1	gelation					118:125	thermally and photo-triggered tandem gelation	81:125	thermally and photo-triggered tandem gelation	81:125	A versatile bioink for three-dimensional printing of cellular scaffolds based on thermally and photo-triggered tandem gelation.
25260606	4	16	theme	poly	564:567	arg1	HA-pNIPAAM					612:621	HA-pNIPAAM	612:621	HA-pNIPAAM	612:621	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	4	16	theme	poly	564:567	arg1	hyaluronan					600:609	the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan	535:609	the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM)	535:622	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	6	17	theme	rheological	908:918	arg1	properties					920:929	rheological properties	908:929	rheological properties	908:929	The bioink was evaluated for rheological properties, swelling behavior, printability and biocompatibility of encapsulated bovine chondrocytes.
25260606	3	18	theme	printing	502:509	arg1	properties					511:520	generally poor printing properties	487:520	generally poor printing properties	487:520	In cartilage engineering, photocrosslinkable glycosaminoglycan-based hydrogels are chondrogenic, but alone have generally poor printing properties.
25260606	0	19	theme	versatile	2:10	arg1	bioink					12:17	A versatile bioink	0:17	A versatile bioink for three-dimensional printing of cellular scaffolds	0:70	A versatile bioink for three-dimensional printing of cellular scaffolds based on thermally and photo-triggered tandem gelation.
25260606	4	20	theme	polymer	556:562	arg1	N-isopropylacrylamide					569:589	N-isopropylacrylamide	569:589	N-isopropylacrylamide	569:589	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	4	20	theme	polymer	556:562	arg1	poly					564:567	the thermoresponsive polymer poly	535:567	the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM)	535:622	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	7	21	theme	good	1086:1089	arg1	viability					1091:1099	good viability	1086:1099	good viability	1086:1099	Elution of HA-pNIPAAM from the scaffold was necessary to obtain good viability.
25260606	2	22	theme	rheological	332:342	arg1	properties					363:372	appropriate rheological and cytocompatible properties	320:372	appropriate rheological and cytocompatible properties	320:372	Printing of viable organ replacements, however, is dependent on bioinks with appropriate rheological and cytocompatible properties.
25260606	4	23	theme	methacrylated	629:641	arg1	HAMA					655:658	HAMA	655:658	HAMA	655:658	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	4	23	theme	methacrylated	629:641	arg1	hyaluronan					643:652	methacrylated hyaluronan	629:652	methacrylated hyaluronan (HAMA)	629:659	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	2	24	theme	appropriate	320:330	arg1	properties					363:372	appropriate rheological and cytocompatible properties	320:372	appropriate rheological and cytocompatible properties	320:372	Printing of viable organ replacements, however, is dependent on bioinks with appropriate rheological and cytocompatible properties.
25260606	1	25	theme	stratified	198:207	arg1	tissues					209:215	stratified tissues	198:215	stratified tissues like articular cartilage	198:240	Layer-by-layer bioprinting is a logical choice for the fabrication of stratified tissues like articular cartilage.
25260606	3	26	theme	photocrosslinkable	401:418	arg1	hydrogels					444:452	photocrosslinkable glycosaminoglycan-based hydrogels	401:452	photocrosslinkable glycosaminoglycan-based hydrogels	401:452	In cartilage engineering, photocrosslinkable glycosaminoglycan-based hydrogels are chondrogenic, but alone have generally poor printing properties.
25260606	7	27	from	scaffold	1053:1060	arg1	Elution					1022:1028	Elution	1022:1028	Elution of HA-pNIPAAM from the scaffold	1022:1060	Elution of HA-pNIPAAM from the scaffold was necessary to obtain good viability.
25260606	1	28	theme	tissues	209:215	arg1	fabrication					183:193	the fabrication	179:193	the fabrication of stratified tissues like articular cartilage	179:240	Layer-by-layer bioprinting is a logical choice for the fabrication of stratified tissues like articular cartilage.
25260606	3	29	theme	glycosaminoglycan-based	420:442	arg1	hydrogels					444:452	photocrosslinkable glycosaminoglycan-based hydrogels	401:452	photocrosslinkable glycosaminoglycan-based hydrogels	401:452	In cartilage engineering, photocrosslinkable glycosaminoglycan-based hydrogels are chondrogenic, but alone have generally poor printing properties.
25260606	6	30	theme	chondrocytes	1008:1019	arg1	biocompatibility					968:983	biocompatibility	968:983	biocompatibility	968:983	The bioink was evaluated for rheological properties, swelling behavior, printability and biocompatibility of encapsulated bovine chondrocytes.
25260606	6	30	theme	chondrocytes	1008:1019	arg1	printability					951:962	printability	951:962	printability	951:962	The bioink was evaluated for rheological properties, swelling behavior, printability and biocompatibility of encapsulated bovine chondrocytes.
25260606	6	30	theme	chondrocytes	1008:1019	arg1	behavior					941:948	behavior	941:948	behavior	941:948	The bioink was evaluated for rheological properties, swelling behavior, printability and biocompatibility of encapsulated bovine chondrocytes.
25260606	6	31	theme	bovine	1001:1006	arg1	chondrocytes					1008:1019	encapsulated bovine chondrocytes	988:1019	encapsulated bovine chondrocytes	988:1019	The bioink was evaluated for rheological properties, swelling behavior, printability and biocompatibility of encapsulated bovine chondrocytes.
25260606	2	32	theme	viable	255:260	arg1	replacements					268:279	viable organ replacements	255:279	viable organ replacements	255:279	Printing of viable organ replacements, however, is dependent on bioinks with appropriate rheological and cytocompatible properties.
25260606	1	33	theme	articular	222:230	arg1	cartilage					232:240	articular cartilage	222:240	articular cartilage	222:240	Layer-by-layer bioprinting is a logical choice for the fabrication of stratified tissues like articular cartilage.
25260606	0	34	theme	three-dimensional	23:39	arg1	printing					41:48	three-dimensional printing	23:48	three-dimensional printing of cellular scaffolds	23:70	A versatile bioink for three-dimensional printing of cellular scaffolds based on thermally and photo-triggered tandem gelation.
25260606	3	35	from	engineering	388:398	arg1	chondrogenic					458:469	chondrogenic	458:469	chondrogenic	458:469	In cartilage engineering, photocrosslinkable glycosaminoglycan-based hydrogels are chondrogenic, but alone have generally poor printing properties.
25260606	8	36	dep	cell-laden	1250:1259	arg1	stratified					1262:1271	stratified	1262:1271	stratified	1262:1271	HA-pNIPAAM can therefore be used to support extrusion of a range of biopolymers which undergo tandem gelation, thereby facilitating the printing of cell-laden, stratified cartilage constructs with zonally varying composition and stiffness.
25260606	3	37	theme	cartilage	378:386	arg1	engineering					388:398	cartilage engineering	378:398	cartilage engineering	378:398	In cartilage engineering, photocrosslinkable glycosaminoglycan-based hydrogels are chondrogenic, but alone have generally poor printing properties.
25260606	0	38	theme	cellular	53:60	arg1	scaffolds					62:70	cellular scaffolds	53:70	cellular scaffolds	53:70	A versatile bioink for three-dimensional printing of cellular scaffolds based on thermally and photo-triggered tandem gelation.
25260606	5	39	theme	long-term	824:832	arg1	stability					845:853	long-term mechanical stability	824:853	long-term mechanical stability	824:853	HA-pNIPAAM provided fast gelation and immediate post-printing structural fidelity, while HAMA ensured long-term mechanical stability upon photocrosslinking.
25260606	5	40	theme	immediate	760:768	arg1	fidelity					795:802	immediate post-printing structural fidelity	760:802	immediate post-printing structural fidelity	760:802	HA-pNIPAAM provided fast gelation and immediate post-printing structural fidelity, while HAMA ensured long-term mechanical stability upon photocrosslinking.
25260606	8	41	theme	cartilage	1273:1281	arg1	constructs					1283:1292	cell-laden, stratified cartilage constructs	1250:1292	cell-laden, stratified cartilage constructs	1250:1292	HA-pNIPAAM can therefore be used to support extrusion of a range of biopolymers which undergo tandem gelation, thereby facilitating the printing of cell-laden, stratified cartilage constructs with zonally varying composition and stiffness.
25260606	5	42	theme	post-printing	770:782	arg1	fidelity					795:802	immediate post-printing structural fidelity	760:802	immediate post-printing structural fidelity	760:802	HA-pNIPAAM provided fast gelation and immediate post-printing structural fidelity, while HAMA ensured long-term mechanical stability upon photocrosslinking.
25260606	4	43	with	scaffolds	678:686	arg1	viability					698:706	good viability	693:706	good viability	693:706	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
25260606	1	44	theme	Layer-by-layer	128:141	arg1	bioprinting					143:153	Layer-by-layer bioprinting	128:153	Layer-by-layer bioprinting	128:153	Layer-by-layer bioprinting is a logical choice for the fabrication of stratified tissues like articular cartilage.
25260606	1	44	theme	Layer-by-layer	128:141	arg1	choice					168:173	a logical choice	158:173	a logical choice for the fabrication of stratified tissues like articular cartilage	158:240	Layer-by-layer bioprinting is a logical choice for the fabrication of stratified tissues like articular cartilage.
25260606	5	45	theme	structural	784:793	arg1	fidelity					795:802	immediate post-printing structural fidelity	760:802	immediate post-printing structural fidelity	760:802	HA-pNIPAAM provided fast gelation and immediate post-printing structural fidelity, while HAMA ensured long-term mechanical stability upon photocrosslinking.
25260606	3	46	contain	have	482:485	arg1	hydrogels					444:452	photocrosslinkable glycosaminoglycan-based hydrogels	401:452	photocrosslinkable glycosaminoglycan-based hydrogels	401:452	In cartilage engineering, photocrosslinkable glycosaminoglycan-based hydrogels are chondrogenic, but alone have generally poor printing properties.
25260606	3	46	contain	have	482:485	arg2	properties					511:520	generally poor printing properties	487:520	generally poor printing properties	487:520	In cartilage engineering, photocrosslinkable glycosaminoglycan-based hydrogels are chondrogenic, but alone have generally poor printing properties.
25260606	2	47	theme	cytocompatible	348:361	arg1	properties					363:372	appropriate rheological and cytocompatible properties	320:372	appropriate rheological and cytocompatible properties	320:372	Printing of viable organ replacements, however, is dependent on bioinks with appropriate rheological and cytocompatible properties.
25260606	8	48	theme	constructs	1283:1292	arg1	printing					1238:1245	the printing	1234:1245	the printing of cell-laden, stratified cartilage constructs	1234:1292	HA-pNIPAAM can therefore be used to support extrusion of a range of biopolymers which undergo tandem gelation, thereby facilitating the printing of cell-laden, stratified cartilage constructs with zonally varying composition and stiffness.
25260606	2	49	theme	replacements	268:279	arg1	Printing					243:250	Printing	243:250	Printing of viable organ replacements	243:279	Printing of viable organ replacements, however, is dependent on bioinks with appropriate rheological and cytocompatible properties.
25260606	8	50	theme	range	1161:1165	arg1	extrusion					1146:1154	extrusion	1146:1154	extrusion of a range of biopolymers which undergo tandem gelation, thereby facilitating the printing of cell-laden, stratified cartilage constructs with zonally varying composition and stiffness	1146:1339	HA-pNIPAAM can therefore be used to support extrusion of a range of biopolymers which undergo tandem gelation, thereby facilitating the printing of cell-laden, stratified cartilage constructs with zonally varying composition and stiffness.
25260606	0	51	theme	scaffolds	62:70	arg1	printing					41:48	three-dimensional printing	23:48	three-dimensional printing of cellular scaffolds	23:70	A versatile bioink for three-dimensional printing of cellular scaffolds based on thermally and photo-triggered tandem gelation.
25260606	3	52	from	chondrogenic	458:469	arg1	engineering					388:398	cartilage engineering	378:398	cartilage engineering	378:398	In cartilage engineering, photocrosslinkable glycosaminoglycan-based hydrogels are chondrogenic, but alone have generally poor printing properties.
25260606	2	53	theme	organ	262:266	arg1	replacements					268:279	viable organ replacements	255:279	viable organ replacements	255:279	Printing of viable organ replacements, however, is dependent on bioinks with appropriate rheological and cytocompatible properties.
25260606	1	54	theme	logical	160:166	arg1	bioprinting					143:153	Layer-by-layer bioprinting	128:153	Layer-by-layer bioprinting	128:153	Layer-by-layer bioprinting is a logical choice for the fabrication of stratified tissues like articular cartilage.
25260606	1	54	theme	logical	160:166	arg1	choice					168:173	a logical choice	158:173	a logical choice for the fabrication of stratified tissues like articular cartilage	158:240	Layer-by-layer bioprinting is a logical choice for the fabrication of stratified tissues like articular cartilage.
25260606	4	55	theme	good	693:696	arg1	viability					698:706	good viability	693:706	good viability	693:706	By blending the thermoresponsive polymer poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) with methacrylated hyaluronan (HAMA), high-resolution scaffolds with good viability were printed.
24095723	5	0	theme	feeding	708:714	arg1	species					665:671	species	665:671	species	665:671	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	5	0	theme	feeding	708:714	arg1	pattern					716:722	feeding pattern	708:722	feeding pattern	708:722	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	2	1	theme	Nectar	108:113	arg1	composition					115:125	Nectar composition	108:125	Nectar composition within a plant pollinator group	108:157	Nectar composition within a plant pollinator group can be variable, and bird pollinated plants can be segregated into two groups based on their adaptations to either a specialist or an occasional bird pollination system.
24095723	6	2	theme	sugar	815:819	arg1	content					821:827	sugar content	815:827	sugar content	815:827	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	8	3	theme	mean	1234:1237	arg1	PGlu					1239:1242	mean PGlu	1234:1242	mean PGlu	1234:1242	The range in mean PGlu was broader in Amethyst Sunbirds (11.52-16.51mmol/L) compared with Cape White-eyes (14.33-15.85mmol/L).
24095723	6	4	dep	nectarivore	959:969	arg1	virens					1000:1005	virens	1000:1005	virens	1000:1005	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	6	5	theme	avian	850:854	arg1	nectarivores					856:867	two avian nectarivores	846:867	two avian nectarivores	846:867	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	9	6	theme	other	1559:1563	arg1	reasons					1551:1557	reasons	1551:1557	reasons other than glucose regulation	1551:1587	This suggests that these birds are not constrained by dietary sugar concentration with regard to PGlu regulation, and consequently selective pressure on plants for their nectar characteristics is due to reasons other than glucose regulation.
24095723	6	7	theme	solution	1038:1045	arg1	diets					1047:1051	sucrose-hexose sugar solution diets	1017:1051	sucrose-hexose sugar solution diets of varying concentrations (5%-35%)	1017:1086	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	1	8	from	levels	48:53	arg1	occasional					61:70	occasional	61:70	occasional	61:70	Blood plasma glucose levels in an occasional and a specialist avian nectarivore.
24095723	1	8	from	levels	48:53	arg1	specialist					78:87	specialist	78:87	specialist	78:87	Blood plasma glucose levels in an occasional and a specialist avian nectarivore.
24095723	3	9	theme	Specialist	329:338	arg1	nectarivores					340:351	Specialist nectarivores	329:351	Specialist nectarivores	329:351	Specialist nectarivores rely primarily on nectar for their energy requirements, while occasional nectarivores meet their energy requirements from nectar as well as from seeds, fruit and insects.
24095723	9	10	theme	PGlu	1445:1448	arg1	regulation					1450:1459	PGlu regulation	1445:1459	PGlu regulation	1445:1459	This suggests that these birds are not constrained by dietary sugar concentration with regard to PGlu regulation, and consequently selective pressure on plants for their nectar characteristics is due to reasons other than glucose regulation.
24095723	2	11	theme	pollinator	142:151	arg1	group					153:157	a plant pollinator group	134:157	a plant pollinator group	134:157	Nectar composition within a plant pollinator group can be variable, and bird pollinated plants can be segregated into two groups based on their adaptations to either a specialist or an occasional bird pollination system.
24095723	9	12	theme	glucose	1570:1576	arg1	regulation					1578:1587	glucose regulation	1570:1587	glucose regulation	1570:1587	This suggests that these birds are not constrained by dietary sugar concentration with regard to PGlu regulation, and consequently selective pressure on plants for their nectar characteristics is due to reasons other than glucose regulation.
24095723	9	13	theme	sugar	1410:1414	arg1	concentration					1416:1428	dietary sugar concentration	1402:1428	dietary sugar concentration	1402:1428	This suggests that these birds are not constrained by dietary sugar concentration with regard to PGlu regulation, and consequently selective pressure on plants for their nectar characteristics is due to reasons other than glucose regulation.
24095723	2	14	theme	plant	136:140	arg1	group					153:157	a plant pollinator group	134:157	a plant pollinator group	134:157	Nectar composition within a plant pollinator group can be variable, and bird pollinated plants can be segregated into two groups based on their adaptations to either a specialist or an occasional bird pollination system.
24095723	6	15	theme	sugar	1032:1036	arg1	diets					1047:1051	sucrose-hexose sugar solution diets	1017:1051	sucrose-hexose sugar solution diets of varying concentrations (5%-35%)	1017:1086	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	3	16	theme	occasional	415:424	arg1	nectarivores					426:437	occasional nectarivores	415:437	occasional nectarivores	415:437	Specialist nectarivores rely primarily on nectar for their energy requirements, while occasional nectarivores meet their energy requirements from nectar as well as from seeds, fruit and insects.
24095723	5	17	theme	ambient	687:693	arg1	species					665:671	species	665:671	species	665:671	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	5	17	theme	ambient	687:693	arg1	temperature					695:705	ambient temperature	687:705	ambient temperature	687:705	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	6	18	theme	sucrose-hexose	1017:1030	arg1	diets					1047:1051	sucrose-hexose sugar solution diets	1017:1051	sucrose-hexose sugar solution diets of varying concentrations (5%-35%)	1017:1086	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	0	19	theme	sugar	5:9	arg1	matter					19:24	sugar content matter	5:24	sugar content matter	5:24	Does sugar content matter?
24095723	4	20	theme	plasma	536:541	arg1	PGlu					566:569	PGlu	566:569	PGlu	566:569	Avian blood plasma glucose concentration (PGlu) is generally high compared with mammals.
24095723	4	20	theme	plasma	536:541	arg1	concentration					551:563	Avian blood plasma glucose concentration	524:563	Avian blood plasma glucose concentration (PGlu)	524:570	Avian blood plasma glucose concentration (PGlu) is generally high compared with mammals.
24095723	6	21	theme	Chalcomitra	916:926	arg1	amethystina					928:938	the Amethyst Sunbird Chalcomitra amethystina	895:938	a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina	870:938	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	7	22	dep	time	1163:1166	arg1	fed					1169:1171	fed	1169:1171	fed	1169:1171	Both species regulated PGlu within a range which was affected by sampling time (fed or fasted) and not dietary sugar concentration.
24095723	7	22	dep	time	1163:1166	arg1	fasted					1176:1181	fasted	1176:1181	fasted	1176:1181	Both species regulated PGlu within a range which was affected by sampling time (fed or fasted) and not dietary sugar concentration.
24095723	6	23	theme	Amethyst	899:906	arg1	amethystina					928:938	the Amethyst Sunbird Chalcomitra amethystina	895:938	a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina	870:938	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	4	24	theme	blood	530:534	arg1	PGlu					566:569	PGlu	566:569	PGlu	566:569	Avian blood plasma glucose concentration (PGlu) is generally high compared with mammals.
24095723	4	24	theme	blood	530:534	arg1	concentration					551:563	Avian blood plasma glucose concentration	524:563	Avian blood plasma glucose concentration (PGlu)	524:570	Avian blood plasma glucose concentration (PGlu) is generally high compared with mammals.
24095723	8	25	from	range	1225:1229	arg1	PGlu					1239:1242	mean PGlu	1234:1242	mean PGlu	1234:1242	The range in mean PGlu was broader in Amethyst Sunbirds (11.52-16.51mmol/L) compared with Cape White-eyes (14.33-15.85mmol/L).
24095723	9	26	theme	selective	1479:1487	arg1	pressure					1489:1496	selective pressure	1479:1496	selective pressure on plants for their nectar characteristics	1479:1539	This suggests that these birds are not constrained by dietary sugar concentration with regard to PGlu regulation, and consequently selective pressure on plants for their nectar characteristics is due to reasons other than glucose regulation.
24095723	4	27	theme	Avian	524:528	arg1	PGlu					566:569	PGlu	566:569	PGlu	566:569	Avian blood plasma glucose concentration (PGlu) is generally high compared with mammals.
24095723	4	27	theme	Avian	524:528	arg1	concentration					551:563	Avian blood plasma glucose concentration	524:563	Avian blood plasma glucose concentration (PGlu)	524:570	Avian blood plasma glucose concentration (PGlu) is generally high compared with mammals.
24095723	3	28	theme	energy	450:455	arg1	requirements					457:468	their energy requirements	444:468	their energy requirements	444:468	Specialist nectarivores rely primarily on nectar for their energy requirements, while occasional nectarivores meet their energy requirements from nectar as well as from seeds, fruit and insects.
24095723	0	29	theme	content	11:17	arg1	matter					19:24	sugar content matter	5:24	sugar content matter	5:24	Does sugar content matter?
24095723	4	30	theme	glucose	543:549	arg1	PGlu					566:569	PGlu	566:569	PGlu	566:569	Avian blood plasma glucose concentration (PGlu) is generally high compared with mammals.
24095723	4	30	theme	glucose	543:549	arg1	concentration					551:563	Avian blood plasma glucose concentration	524:563	Avian blood plasma glucose concentration (PGlu)	524:570	Avian blood plasma glucose concentration (PGlu) is generally high compared with mammals.
24095723	1	31	theme	avian	89:93	arg1	nectarivore					95:105	avian nectarivore	89:105	avian nectarivore	89:105	Blood plasma glucose levels in an occasional and a specialist avian nectarivore.
24095723	8	32	dep	broader	1248:1254	arg1	11.52-16.51mmol/L					1278:1294	11.52-16.51mmol/L	1278:1294	11.52-16.51mmol/L	1278:1294	The range in mean PGlu was broader in Amethyst Sunbirds (11.52-16.51mmol/L) compared with Cape White-eyes (14.33-15.85mmol/L).
24095723	3	33	theme	energy	388:393	arg1	requirements					395:406	their energy requirements	382:406	their energy requirements	382:406	Specialist nectarivores rely primarily on nectar for their energy requirements, while occasional nectarivores meet their energy requirements from nectar as well as from seeds, fruit and insects.
24095723	2	34	theme	bird	304:307	arg1	system					321:326	an occasional bird pollination system	290:326	an occasional bird pollination system	290:326	Nectar composition within a plant pollinator group can be variable, and bird pollinated plants can be segregated into two groups based on their adaptations to either a specialist or an occasional bird pollination system.
24095723	7	35	theme	dietary	1192:1198	arg1	concentration					1206:1218	dietary sugar concentration	1192:1218	sampling time (fed or fasted) and not dietary sugar concentration	1154:1218	Both species regulated PGlu within a range which was affected by sampling time (fed or fasted) and not dietary sugar concentration.
24095723	5	36	theme	reproductive	725:736	arg1	status					738:743	reproductive status	725:743	reproductive status	725:743	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	5	36	theme	reproductive	725:736	arg1	species					665:671	species	665:671	species	665:671	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	6	37	dep	nectarivore	883:893	arg1	amethystina					928:938	the Amethyst Sunbird Chalcomitra amethystina	895:938	a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina	870:938	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	7	38	theme	sugar	1200:1204	arg1	concentration					1206:1218	dietary sugar concentration	1192:1218	sampling time (fed or fasted) and not dietary sugar concentration	1154:1218	Both species regulated PGlu within a range which was affected by sampling time (fed or fasted) and not dietary sugar concentration.
24095723	6	39	theme	specialist	872:881	arg1	nectarivore					883:893	a specialist nectarivore	870:893	a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina	870:938	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	5	40	theme	moult	767:771	arg1	species					665:671	species	665:671	species	665:671	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	5	40	theme	moult	767:771	arg1	status					773:778	moult status	767:778	moult status	767:778	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	6	41	theme	Sunbird	908:914	arg1	amethystina					928:938	the Amethyst Sunbird Chalcomitra amethystina	895:938	a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina	870:938	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	8	42	theme	Cape	1311:1314	arg1	14.33-15.85mmol/L					1328:1344	14.33-15.85mmol/L	1328:1344	14.33-15.85mmol/L	1328:1344	The range in mean PGlu was broader in Amethyst Sunbirds (11.52-16.51mmol/L) compared with Cape White-eyes (14.33-15.85mmol/L).
24095723	8	42	theme	Cape	1311:1314	arg1	White-eyes					1316:1325	Cape White-eyes	1311:1325	Cape White-eyes (14.33-15.85mmol/L)	1311:1345	The range in mean PGlu was broader in Amethyst Sunbirds (11.52-16.51mmol/L) compared with Cape White-eyes (14.33-15.85mmol/L).
24095723	2	43	theme	pollination	309:319	arg1	system					321:326	an occasional bird pollination system	290:326	an occasional bird pollination system	290:326	Nectar composition within a plant pollinator group can be variable, and bird pollinated plants can be segregated into two groups based on their adaptations to either a specialist or an occasional bird pollination system.
24095723	2	44	theme	pollinated	185:194	arg1	plants					196:201	bird pollinated plants	180:201	bird pollinated plants	180:201	Nectar composition within a plant pollinator group can be variable, and bird pollinated plants can be segregated into two groups based on their adaptations to either a specialist or an occasional bird pollination system.
24095723	9	45	theme	nectar	1518:1523	arg1	characteristics					1525:1539	their nectar characteristics	1512:1539	their nectar characteristics	1512:1539	This suggests that these birds are not constrained by dietary sugar concentration with regard to PGlu regulation, and consequently selective pressure on plants for their nectar characteristics is due to reasons other than glucose regulation.
24095723	1	46	dep	occasional	61:70	arg1	nectarivore					95:105	avian nectarivore	89:105	avian nectarivore	89:105	Blood plasma glucose levels in an occasional and a specialist avian nectarivore.
24095723	9	47	from	pressure	1489:1496	arg1	plants					1501:1506	plants	1501:1506	plants	1501:1506	This suggests that these birds are not constrained by dietary sugar concentration with regard to PGlu regulation, and consequently selective pressure on plants for their nectar characteristics is due to reasons other than glucose regulation.
24095723	2	48	theme	bird	180:183	arg1	plants					196:201	bird pollinated plants	180:201	bird pollinated plants	180:201	Nectar composition within a plant pollinator group can be variable, and bird pollinated plants can be segregated into two groups based on their adaptations to either a specialist or an occasional bird pollination system.
24095723	2	49	theme	occasional	293:302	arg1	system					321:326	an occasional bird pollination system	290:326	an occasional bird pollination system	290:326	Nectar composition within a plant pollinator group can be variable, and bird pollinated plants can be segregated into two groups based on their adaptations to either a specialist or an occasional bird pollination system.
24095723	5	50	theme	factors	647:653	arg1	species					665:671	species	665:671	species	665:671	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	5	50	theme	factors	647:653	arg1	range					638:642	a range	636:642	a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others	636:792	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	1	51	theme	Blood	27:31	arg1	levels					48:53	Blood plasma glucose levels	27:53	Blood plasma glucose levels in an occasional and a specialist avian nectarivore.	27:106	Blood plasma glucose levels in an occasional and a specialist avian nectarivore.
24095723	6	52	theme	concentrations	1064:1077	arg1	diets					1047:1051	sucrose-hexose sugar solution diets	1017:1051	sucrose-hexose sugar solution diets of varying concentrations (5%-35%)	1017:1086	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	6	53	theme	%	1081:1081	arg1	%					1085:1085	5%-35%	1080:1085	5%-35%	1080:1085	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	6	53	theme	%	1081:1081	arg1	concentrations					1064:1077	varying concentrations	1056:1077	varying concentrations (5%-35%)	1056:1086	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	9	54	theme	dietary	1402:1408	arg1	concentration					1416:1428	dietary sugar concentration	1402:1428	dietary sugar concentration	1402:1428	This suggests that these birds are not constrained by dietary sugar concentration with regard to PGlu regulation, and consequently selective pressure on plants for their nectar characteristics is due to reasons other than glucose regulation.
24095723	1	55	theme	plasma	33:38	arg1	levels					48:53	Blood plasma glucose levels	27:53	Blood plasma glucose levels in an occasional and a specialist avian nectarivore.	27:106	Blood plasma glucose levels in an occasional and a specialist avian nectarivore.
24095723	6	56	theme	varying	1056:1062	arg1	%					1085:1085	5%-35%	1080:1085	5%-35%	1080:1085	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	6	56	theme	varying	1056:1062	arg1	concentrations					1064:1077	varying concentrations	1056:1077	varying concentrations (5%-35%)	1056:1086	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	7	57	theme	sampling	1154:1161	arg1	time					1163:1166	sampling time	1154:1166	sampling time (fed or fasted) and not dietary sugar concentration	1154:1218	Both species regulated PGlu within a range which was affected by sampling time (fed or fasted) and not dietary sugar concentration.
24095723	6	58	theme	nectarivores	856:867	arg1	nectarivore					883:893	a specialist nectarivore	870:893	a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina	870:938	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	6	58	theme	nectarivores	856:867	arg1	nectarivore					959:969	an occasional nectarivore	945:969	an occasional nectarivore the Cape White-eye Zosterops virens	945:1005	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	6	58	theme	nectarivores	856:867	arg1	PGlu					838:841	PGlu	838:841	PGlu of two avian nectarivores	838:867	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
24095723	1	59	theme	glucose	40:46	arg1	levels					48:53	Blood plasma glucose levels	27:53	Blood plasma glucose levels in an occasional and a specialist avian nectarivore.	27:106	Blood plasma glucose levels in an occasional and a specialist avian nectarivore.
24095723	5	60	theme	circadian	746:754	arg1	rhythm					756:761	circadian rhythm	746:761	circadian rhythm	746:761	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	5	60	theme	circadian	746:754	arg1	species					665:671	species	665:671	species	665:671	It is also affected by a range of factors including species, gender, age, ambient temperature, feeding pattern, reproductive status, circadian rhythm and moult status, among others.
24095723	6	61	theme	occasional	948:957	arg1	nectarivore					959:969	an occasional nectarivore	945:969	an occasional nectarivore the Cape White-eye Zosterops virens	945:1005	We examined whether sugar content affected PGlu of two avian nectarivores, a specialist nectarivore the Amethyst Sunbird Chalcomitra amethystina, and an occasional nectarivore the Cape White-eye Zosterops virens, when fed sucrose-hexose sugar solution diets of varying concentrations (5%-35%).
27074852	1	0	theme	oxide	219:223	arg1	nanoparticles					232:244	controlled cuprous oxide (Cu2O) nanoparticles	200:244	controlled cuprous oxide (Cu2O) nanoparticles	200:244	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	1	1	theme	temperature	139:149	arg1	effect					113:118	the effect	109:118	the effect of reducing agent, temperature and pH of the reaction	109:172	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	2	2	theme	surface	256:262	arg1	method					264:269	Response surface method	247:269	Response surface method of the ultraviolet-visible spectroscopy	247:309	Response surface method of the ultraviolet-visible spectroscopy is allowed to determine the most relevant factors for the size distribution of the nanoCu2O.
27074852	2	3	theme	ultraviolet-visible	278:296	arg1	spectroscopy					298:309	the ultraviolet-visible spectroscopy	274:309	the ultraviolet-visible spectroscopy	274:309	Response surface method of the ultraviolet-visible spectroscopy is allowed to determine the most relevant factors for the size distribution of the nanoCu2O.
27074852	2	4	theme	Response	247:254	arg1	method					264:269	Response surface method	247:269	Response surface method of the ultraviolet-visible spectroscopy	247:309	Response surface method of the ultraviolet-visible spectroscopy is allowed to determine the most relevant factors for the size distribution of the nanoCu2O.
27074852	3	5	theme	cubic	450:454	arg1	structure					456:464	a cubic structure	448:464	a cubic structure	448:464	X-ray diffraction reflections correspond to a cubic structure, with sizes from 31.9 to 104.3 nm.
27074852	2	6	theme	size	369:372	arg1	distribution					374:385	the size distribution	365:385	the size distribution of the nanoCu2O	365:401	Response surface method of the ultraviolet-visible spectroscopy is allowed to determine the most relevant factors for the size distribution of the nanoCu2O.
27074852	4	7	theme	different	567:575	arg1	shapes					577:582	the different shapes	563:582	the different shapes	563:582	High-resolution transmission electron microscopy reveals that the different shapes depend strongly on the conditions of the green synthesis.
27074852	1	8	theme	central	71:77	arg1	design					89:94	a central composite design	69:94	a central composite design	69:94	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	1	9	theme	pH	155:156	arg1	effect					113:118	the effect	109:118	the effect of reducing agent, temperature and pH of the reaction	109:172	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	1	10	theme	composite	79:87	arg1	design					89:94	a central composite design	69:94	a central composite design	69:94	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	3	11	theme	X-ray	404:408	arg1	reflections					422:432	X-ray diffraction reflections	404:432	X-ray diffraction reflections	404:432	X-ray diffraction reflections correspond to a cubic structure, with sizes from 31.9 to 104.3 nm.
27074852	4	12	theme	electron	530:537	arg1	microscopy					539:548	High-resolution transmission electron microscopy	501:548	High-resolution transmission electron microscopy	501:548	High-resolution transmission electron microscopy reveals that the different shapes depend strongly on the conditions of the green synthesis.
27074852	2	13	theme	nanoCu2O	394:401	arg1	distribution					374:385	the size distribution	365:385	the size distribution of the nanoCu2O	365:401	Response surface method of the ultraviolet-visible spectroscopy is allowed to determine the most relevant factors for the size distribution of the nanoCu2O.
27074852	3	14	dep	104.3	491:495	arg1	to					488:489	to	488:489	to	488:489	X-ray diffraction reflections correspond to a cubic structure, with sizes from 31.9 to 104.3 nm.
27074852	1	15	theme	reducing	123:130	arg1	agent					132:136	reducing agent	123:136	reducing agent	123:136	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	0	16	theme	oxide	42:46	arg1	synthesis					16:24	synthesis	16:24	synthesis of nano-cuprous oxide	16:46	Green chemistry synthesis of nano-cuprous oxide.
27074852	4	17	theme	synthesis	631:639	arg1	conditions					607:616	the conditions	603:616	the conditions of the green synthesis	603:639	High-resolution transmission electron microscopy reveals that the different shapes depend strongly on the conditions of the green synthesis.
27074852	0	18	theme	nano-cuprous	29:40	arg1	oxide					42:46	nano-cuprous oxide	29:46	nano-cuprous oxide	29:46	Green chemistry synthesis of nano-cuprous oxide.
27074852	4	19	theme	green	625:629	arg1	synthesis					631:639	the green synthesis	621:639	the green synthesis	621:639	High-resolution transmission electron microscopy reveals that the different shapes depend strongly on the conditions of the green synthesis.
27074852	3	20	theme	diffraction	410:420	arg1	reflections					422:432	X-ray diffraction reflections	404:432	X-ray diffraction reflections	404:432	X-ray diffraction reflections correspond to a cubic structure, with sizes from 31.9 to 104.3 nm.
27074852	4	21	theme	transmission	517:528	arg1	microscopy					539:548	High-resolution transmission electron microscopy	501:548	High-resolution transmission electron microscopy	501:548	High-resolution transmission electron microscopy reveals that the different shapes depend strongly on the conditions of the green synthesis.
27074852	4	22	theme	High-resolution	501:515	arg1	microscopy					539:548	High-resolution transmission electron microscopy	501:548	High-resolution transmission electron microscopy	501:548	High-resolution transmission electron microscopy reveals that the different shapes depend strongly on the conditions of the green synthesis.
27074852	1	23	dep	chemistry	55:63	arg1	evaluate					100:107	evaluate	100:107	to evaluate the effect of reducing agent, temperature and pH of the reaction	97:172	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	2	24	theme	spectroscopy	298:309	arg1	method					264:269	Response surface method	247:269	Response surface method of the ultraviolet-visible spectroscopy	247:309	Response surface method of the ultraviolet-visible spectroscopy is allowed to determine the most relevant factors for the size distribution of the nanoCu2O.
27074852	1	25	theme	reaction	165:172	arg1	effect					113:118	the effect	109:118	the effect of reducing agent, temperature and pH of the reaction	109:172	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	2	26	theme	relevant	344:351	arg1	factors					353:359	the most relevant factors	335:359	the most relevant factors for the size distribution of the nanoCu2O	335:401	Response surface method of the ultraviolet-visible spectroscopy is allowed to determine the most relevant factors for the size distribution of the nanoCu2O.
27074852	1	27	theme	controlled	200:209	arg1	Cu2O					226:229	Cu2O	226:229	Cu2O	226:229	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	1	27	theme	controlled	200:209	arg1	oxide					219:223	controlled cuprous oxide	200:223	controlled cuprous oxide (Cu2O) nanoparticles	200:244	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	1	28	theme	Green	49:53	arg1	chemistry					55:63	Green chemistry	49:63	Green chemistry	49:63	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	1	29	theme	agent	132:136	arg1	effect					113:118	the effect	109:118	the effect of reducing agent, temperature and pH of the reaction	109:172	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	3	30	from	nm	497:498	arg1	sizes					472:476	sizes	472:476	sizes from 31.9 to 104.3 nm	472:498	X-ray diffraction reflections correspond to a cubic structure, with sizes from 31.9 to 104.3 nm.
27074852	1	31	theme	cuprous	211:217	arg1	Cu2O					226:229	Cu2O	226:229	Cu2O	226:229	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
27074852	1	31	theme	cuprous	211:217	arg1	oxide					219:223	controlled cuprous oxide	200:223	controlled cuprous oxide (Cu2O) nanoparticles	200:244	Green chemistry and a central composite design, to evaluate the effect of reducing agent, temperature and pH of the reaction, were employed to produce controlled cuprous oxide (Cu2O) nanoparticles.
25921001	7	0	theme	dEAC	1496:1499	arg1	matrix					1501:1506	either dEAC matrix	1489:1506	either dEAC matrix which was also displayed by an intact endothelial lining after seeding	1489:1577	Cytotoxicity was not observed in either dEAC matrix which was also displayed by an intact endothelial lining after seeding.
25921001	4	1	theme	suture	668:673	arg1	strength					685:692	suture retention strength	668:692	suture retention strength	668:692	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	1	theme	suture	668:673	arg1	characteristics					651:665	their biomechanical characteristics	631:665	their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure)	631:795	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	6	2	theme	Matrix	1294:1299	arg1	characterization					1301:1316	Matrix characterization	1294:1316	Matrix characterization	1294:1316	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	4	3	theme	arterial	744:751	arg1	pressure					787:794	arterial and venous systolic and diastolic pressure	744:794	arterial and venous systolic and diastolic pressure	744:794	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	8	4	theme	shunts	1744:1749	arg1	generation					1716:1725	the generation	1712:1725	the generation of haemodialysis shunts	1712:1749	Thus, even intensified decellularization generates matrix scaffolds highly suitable for vascular tissue engineering purposes, e.g., the generation of haemodialysis shunts.
25921001	4	5	dep	cytotoxicity	881:892	arg1	assay					901:905	WST-8 assay	895:905	WST-8 assay	895:905	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	5	dep	cytotoxicity	881:892	arg1	seeding					928:934	endothelial cell seeding	911:934	endothelial cell seeding	911:934	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	5	6	contain	had	1231:1233	arg2	effect					1238:1243	no effect	1235:1243	no effect	1235:1243	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	6	contain	had	1231:1233	arg1	protocols					1048:1056	Both decellularization protocols	1025:1056	Both decellularization protocols	1025:1056	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	4	7	theme	venous	757:762	arg1	pressure					787:794	arterial and venous systolic and diastolic pressure	744:794	arterial and venous systolic and diastolic pressure	744:794	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	2	8	theme	intensified	311:321	arg1	treatment					333:341	intensified detergent treatment	311:341	intensified detergent treatment	311:341	Recently it was shown that intensified detergent treatment completely removed cellular components from dEAC and thereby significantly reduced matrix immunogenicity.
25921001	1	9	theme	vascular	214:221	arg1	grafts					223:228	alloplastic vascular grafts	202:228	alloplastic vascular grafts in haemodialysis patients	202:254	Decellularized equine carotid arteries (dEAC) are suggested to represent an alternative for alloplastic vascular grafts in haemodialysis patients to achieve vascular access.
25921001	6	10	dep	%	1365:1365	arg1	int-dEAC					1389:1396	int-dEAC	1389:1396	int-dEAC	1389:1396	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	6	10	dep	%	1365:1365	arg1	p					1399:1399	p < 0.01	1399:1406	p < 0.01	1399:1406	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	6	10	dep	%	1365:1365	arg1	con-dEAC					1368:1375	con-dEAC	1368:1375	con-dEAC	1368:1375	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	6	11	theme	39.0	1361:1364	arg1	%					1365:1365	%	1365:1365	%	1365:1365	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	4	12	dep	characteristics	651:665	arg1	strength					685:692	suture retention strength	668:692	suture retention strength	668:692	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	12	dep	characteristics	651:665	arg1	pressure					701:708	burst pressure	695:708	burst pressure	695:708	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	12	dep	characteristics	651:665	arg1	compliance					730:739	circumferential compliance	714:739	circumferential compliance	714:739	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	12	dep	characteristics	651:665	arg1	characteristics					651:665	their biomechanical characteristics	631:665	their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure)	631:795	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	6	13	theme	glycosaminoglycans	1422:1439	arg1	reduction					1409:1417	a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction	1359:1417	a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans	1359:1439	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	1	14	from	grafts	223:228	arg1	patients					247:254	haemodialysis patients	233:254	haemodialysis patients	233:254	Decellularized equine carotid arteries (dEAC) are suggested to represent an alternative for alloplastic vascular grafts in haemodialysis patients to achieve vascular access.
25921001	5	15	dep	reduced	1069:1075	arg1	compliance					1112:1121	venous pressure compliance	1096:1121	venous pressure compliance	1096:1121	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	16	theme	27.4	1133:1136	arg1	n-EAC					1142:1146	n-EAC	1142:1146	n-EAC	1142:1146	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	16	theme	27.4	1133:1136	arg1	%					1137:1137	32.2 and 27.4%	1124:1137	%	1137:1137	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	0	17	dep	Scaffold	70:77	arg1	Biomechanics					79:90	Biomechanics	79:90	Biomechanics	79:90	Effect of Intensified Decellularization of Equine Carotid Arteries on Scaffold Biomechanics and Cytotoxicity.
25921001	6	18	theme	collagen	1337:1344	arg1	contents					1346:1353	unchanged collagen contents	1327:1353	unchanged collagen contents	1327:1353	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	5	19	theme	burst	1278:1282	arg1	pressure					1284:1291	burst pressure	1278:1291	burst pressure	1278:1291	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	7	20	located	observed	1477:1484	arg1	matrix					1501:1506	either dEAC matrix	1489:1506	either dEAC matrix which was also displayed by an intact endothelial lining after seeding	1489:1577	Cytotoxicity was not observed in either dEAC matrix which was also displayed by an intact endothelial lining after seeding.
25921001	7	20	located	observed	1477:1484	arg2	Cytotoxicity					1456:1467	Cytotoxicity	1456:1467	Cytotoxicity	1456:1467	Cytotoxicity was not observed in either dEAC matrix which was also displayed by an intact endothelial lining after seeding.
25921001	0	21	from	Effect	0:5	arg1	Scaffold					70:77	Scaffold	70:77	Scaffold	70:77	Effect of Intensified Decellularization of Equine Carotid Arteries on Scaffold Biomechanics and Cytotoxicity.
25921001	0	21	from	Effect	0:5	arg1	Cytotoxicity					96:107	Cytotoxicity	96:107	Cytotoxicity	96:107	Effect of Intensified Decellularization of Equine Carotid Arteries on Scaffold Biomechanics and Cytotoxicity.
25921001	5	22	theme	decellularization	1030:1046	arg1	protocols					1048:1056	Both decellularization protocols	1025:1056	Both decellularization protocols	1025:1056	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	6	23	theme	26.4	1382:1385	arg1	%					1386:1386	%	1386:1386	%	1386:1386	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	4	24	theme	indirect	861:868	arg1	cytotoxicity					881:892	indirect and direct cytotoxicity	861:892	indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding)	861:935	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	3	25	theme	matrix	485:490	arg1	composition					492:502	matrix composition	485:502	matrix composition	485:502	However, detergents may also affect matrix composition and stability and render scaffolds cytotoxic.
25921001	5	26	dep	%	1137:1137	arg1	n-EAC					1210:1214	n-EAC	1210:1214	n-EAC	1210:1214	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	26	dep	%	1137:1137	arg1	p					1149:1149	p < 0.01	1149:1156	p < 0.01	1149:1156	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	26	dep	%	1137:1137	arg1	%					1205:1205	26.8 and 23.7%	1192:1205	32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC	1124:1214	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	26	dep	%	1137:1137	arg1	compliance					1180:1189	arterial pressure compliance	1162:1189	arterial pressure compliance	1162:1189	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	27	theme	pressure	1171:1178	arg1	compliance					1180:1189	arterial pressure compliance	1162:1189	arterial pressure compliance	1162:1189	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	28	theme	matrix	1077:1082	arg1	compliance					1084:1093	matrix compliance	1077:1093	matrix compliance	1077:1093	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	2	29	theme	matrix	426:431	arg1	immunogenicity					433:446	significantly reduced matrix immunogenicity	404:446	significantly reduced matrix immunogenicity	404:446	Recently it was shown that intensified detergent treatment completely removed cellular components from dEAC and thereby significantly reduced matrix immunogenicity.
25921001	5	30	theme	p	1217:1217	arg1	<					1219:1219	p < 0.01	1217:1224	p < 0.01	1217:1224	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	8	31	dep	generation	1716:1725	arg1	e.g.					1706:1709	e.g.	1706:1709	e.g.	1706:1709	Thus, even intensified decellularization generates matrix scaffolds highly suitable for vascular tissue engineering purposes, e.g., the generation of haemodialysis shunts.
25921001	4	32	theme	glycosaminoglycan	830:846	arg1	content					848:854	glycosaminoglycan content	830:854	glycosaminoglycan content	830:854	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	32	theme	glycosaminoglycan	830:846	arg1	components					805:814	matrix components	798:814	matrix components (collagen and glycosaminoglycan content)	798:855	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	0	33	theme	Intensified	10:20	arg1	Decellularization					22:38	Intensified Decellularization	10:38	Intensified Decellularization of Equine Carotid Arteries	10:65	Effect of Intensified Decellularization of Equine Carotid Arteries on Scaffold Biomechanics and Cytotoxicity.
25921001	1	34	theme	equine	125:130	arg1	dEAC					150:153	dEAC	150:153	dEAC	150:153	Decellularized equine carotid arteries (dEAC) are suggested to represent an alternative for alloplastic vascular grafts in haemodialysis patients to achieve vascular access.
25921001	1	34	theme	equine	125:130	arg1	arteries					140:147	Decellularized equine carotid arteries	110:147	Decellularized equine carotid arteries (dEAC)	110:154	Decellularized equine carotid arteries (dEAC) are suggested to represent an alternative for alloplastic vascular grafts in haemodialysis patients to achieve vascular access.
25921001	4	35	dep	native	955:960	arg1	n-EAC					963:967	n-EAC	963:967	n-EAC	963:967	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	36	theme	endothelial	911:921	arg1	seeding					928:934	endothelial cell seeding	911:934	endothelial cell seeding	911:934	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	6	37	theme	<	1401:1401	arg1	int-dEAC					1389:1396	int-dEAC	1389:1396	int-dEAC	1389:1396	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	6	37	theme	<	1401:1401	arg1	p					1399:1399	p < 0.01	1399:1406	p < 0.01	1399:1406	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	5	38	theme	n-EAC	1210:1214	arg1	n-EAC					1210:1214	n-EAC	1210:1214	n-EAC	1210:1214	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	38	theme	n-EAC	1210:1214	arg1	%					1205:1205	26.8 and 23.7%	1192:1205	32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC	1124:1214	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	4	39	theme	direct	874:879	arg1	cytotoxicity					881:892	indirect and direct cytotoxicity	861:892	indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding)	861:935	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	2	40	theme	cellular	362:369	arg1	components					371:380	cellular components	362:380	cellular components	362:380	Recently it was shown that intensified detergent treatment completely removed cellular components from dEAC and thereby significantly reduced matrix immunogenicity.
25921001	8	41	theme	vascular	1668:1675	arg1	purposes					1696:1703	vascular tissue engineering purposes	1668:1703	vascular tissue engineering purposes	1668:1703	Thus, even intensified decellularization generates matrix scaffolds highly suitable for vascular tissue engineering purposes, e.g., the generation of haemodialysis shunts.
25921001	1	42	theme	haemodialysis	233:245	arg1	patients					247:254	haemodialysis patients	233:254	haemodialysis patients	233:254	Decellularized equine carotid arteries (dEAC) are suggested to represent an alternative for alloplastic vascular grafts in haemodialysis patients to achieve vascular access.
25921001	8	43	theme	intensified	1591:1601	arg1	decellularization					1603:1619	even intensified decellularization	1586:1619	even intensified decellularization	1586:1619	Thus, even intensified decellularization generates matrix scaffolds highly suitable for vascular tissue engineering purposes, e.g., the generation of haemodialysis shunts.
25921001	4	44	theme	burst	695:699	arg1	pressure					701:708	burst pressure	695:708	burst pressure	695:708	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	44	theme	burst	695:699	arg1	characteristics					651:665	their biomechanical characteristics	631:665	their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure)	631:795	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	8	45	theme	engineering	1684:1694	arg1	purposes					1696:1703	vascular tissue engineering purposes	1668:1703	vascular tissue engineering purposes	1668:1703	Thus, even intensified decellularization generates matrix scaffolds highly suitable for vascular tissue engineering purposes, e.g., the generation of haemodialysis shunts.
25921001	5	46	theme	retention	1255:1263	arg1	strength					1265:1272	suture retention strength	1248:1272	suture retention strength	1248:1272	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	47	theme	pressure	1103:1110	arg1	compliance					1112:1121	venous pressure compliance	1096:1121	venous pressure compliance	1096:1121	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	6	48	theme	unchanged	1327:1335	arg1	contents					1346:1353	unchanged collagen contents	1327:1353	unchanged collagen contents	1327:1353	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	1	49	theme	vascular	267:274	arg1	access					276:281	vascular access	267:281	vascular access	267:281	Decellularized equine carotid arteries (dEAC) are suggested to represent an alternative for alloplastic vascular grafts in haemodialysis patients to achieve vascular access.
25921001	4	50	theme	biomechanical	637:649	arg1	strength					685:692	suture retention strength	668:692	suture retention strength	668:692	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	50	theme	biomechanical	637:649	arg1	pressure					701:708	burst pressure	695:708	burst pressure	695:708	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	50	theme	biomechanical	637:649	arg1	compliance					730:739	circumferential compliance	714:739	circumferential compliance	714:739	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	50	theme	biomechanical	637:649	arg1	characteristics					651:665	their biomechanical characteristics	631:665	their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure)	631:795	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	51	theme	native	955:960	arg1	carotids					1004:1011	native (n-EAC) and conventionally decellularized carotids	955:1011	native (n-EAC) and conventionally decellularized carotids (con-dEAC)	955:1022	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	51	theme	native	955:960	arg1	con-dEAC					1014:1021	con-dEAC	1014:1021	con-dEAC	1014:1021	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	8	52	theme	suitable	1655:1662	arg1	scaffolds					1638:1646	matrix scaffolds	1631:1646	matrix scaffolds	1631:1646	Thus, even intensified decellularization generates matrix scaffolds highly suitable for vascular tissue engineering purposes, e.g., the generation of haemodialysis shunts.
25921001	5	53	dep	compliance	1112:1121	arg1	<					1219:1219	p < 0.01	1217:1224	p < 0.01	1217:1224	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	53	dep	compliance	1112:1121	arg1	n-EAC					1142:1146	n-EAC	1142:1146	n-EAC	1142:1146	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	53	dep	compliance	1112:1121	arg1	%					1137:1137	32.2 and 27.4%	1124:1137	%	1137:1137	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	0	54	theme	Carotid	50:56	arg1	Arteries					58:65	Equine Carotid Arteries	43:65	Equine Carotid Arteries	43:65	Effect of Intensified Decellularization of Equine Carotid Arteries on Scaffold Biomechanics and Cytotoxicity.
25921001	4	55	theme	cell	923:926	arg1	seeding					928:934	endothelial cell seeding	911:934	endothelial cell seeding	911:934	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	8	56	theme	haemodialysis	1730:1742	arg1	shunts					1744:1749	haemodialysis shunts	1730:1749	haemodialysis shunts	1730:1749	Thus, even intensified decellularization generates matrix scaffolds highly suitable for vascular tissue engineering purposes, e.g., the generation of haemodialysis shunts.
25921001	2	57	theme	detergent	323:331	arg1	treatment					333:341	intensified detergent treatment	311:341	intensified detergent treatment	311:341	Recently it was shown that intensified detergent treatment completely removed cellular components from dEAC and thereby significantly reduced matrix immunogenicity.
25921001	1	58	theme	alloplastic	202:212	arg1	grafts					223:228	alloplastic vascular grafts	202:228	alloplastic vascular grafts in haemodialysis patients	202:254	Decellularized equine carotid arteries (dEAC) are suggested to represent an alternative for alloplastic vascular grafts in haemodialysis patients to achieve vascular access.
25921001	4	59	theme	decellularized	989:1002	arg1	carotids					1004:1011	native (n-EAC) and conventionally decellularized carotids	955:1011	native (n-EAC) and conventionally decellularized carotids (con-dEAC)	955:1022	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	59	theme	decellularized	989:1002	arg1	con-dEAC					1014:1021	con-dEAC	1014:1021	con-dEAC	1014:1021	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	6	60	theme	%	1365:1365	arg1	reduction					1409:1417	a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction	1359:1417	a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans	1359:1439	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	4	61	theme	circumferential	714:728	arg1	compliance					730:739	circumferential compliance	714:739	circumferential compliance	714:739	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	61	theme	circumferential	714:728	arg1	characteristics					651:665	their biomechanical characteristics	631:665	their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure)	631:795	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	1	62	theme	carotid	132:138	arg1	dEAC					150:153	dEAC	150:153	dEAC	150:153	Decellularized equine carotid arteries (dEAC) are suggested to represent an alternative for alloplastic vascular grafts in haemodialysis patients to achieve vascular access.
25921001	1	62	theme	carotid	132:138	arg1	arteries					140:147	Decellularized equine carotid arteries	110:147	Decellularized equine carotid arteries (dEAC)	110:154	Decellularized equine carotid arteries (dEAC) are suggested to represent an alternative for alloplastic vascular grafts in haemodialysis patients to achieve vascular access.
25921001	4	63	theme	decellularized	573:586	arg1	int-dEAC					598:605	int-dEAC	598:605	int-dEAC	598:605	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	63	theme	decellularized	573:586	arg1	carotids					588:595	intensively decellularized carotids	561:595	intensively decellularized carotids (int-dEAC)	561:606	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	64	theme	matrix	798:803	arg1	collagen					817:824	collagen	817:824	collagen	817:824	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	64	theme	matrix	798:803	arg1	content					848:854	glycosaminoglycan content	830:854	glycosaminoglycan content	830:854	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	64	theme	matrix	798:803	arg1	components					805:814	matrix components	798:814	matrix components (collagen and glycosaminoglycan content)	798:855	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	5	65	theme	n-EAC	1142:1146	arg1	n-EAC					1142:1146	n-EAC	1142:1146	n-EAC	1142:1146	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	65	theme	n-EAC	1142:1146	arg1	%					1137:1137	32.2 and 27.4%	1124:1137	%	1137:1137	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	4	66	theme	systolic	764:771	arg1	pressure					787:794	arterial and venous systolic and diastolic pressure	744:794	arterial and venous systolic and diastolic pressure	744:794	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	67	theme	diastolic	777:785	arg1	pressure					787:794	arterial and venous systolic and diastolic pressure	744:794	arterial and venous systolic and diastolic pressure	744:794	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	6	68	theme	%	1386:1386	arg1	reduction					1409:1417	a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction	1359:1417	a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans	1359:1439	Matrix characterization revealed unchanged collagen contents but a 39.0% (con-dEAC) and 26.4% (int-dEAC, p < 0.01) reduction of glycosaminoglycans, respectively.
25921001	7	69	theme	endothelial	1546:1556	arg1	lining					1558:1563	an intact endothelial lining	1536:1563	an intact endothelial lining after seeding	1536:1577	Cytotoxicity was not observed in either dEAC matrix which was also displayed by an intact endothelial lining after seeding.
25921001	5	70	theme	arterial	1162:1169	arg1	compliance					1180:1189	arterial pressure compliance	1162:1189	arterial pressure compliance	1162:1189	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	4	71	theme	retention	675:683	arg1	strength					685:692	suture retention strength	668:692	suture retention strength	668:692	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	71	theme	retention	675:683	arg1	characteristics					651:665	their biomechanical characteristics	631:665	their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure)	631:795	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	72	dep	components	805:814	arg1	collagen					817:824	collagen	817:824	collagen	817:824	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	72	dep	components	805:814	arg1	content					848:854	glycosaminoglycan content	830:854	glycosaminoglycan content	830:854	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	72	dep	components	805:814	arg1	components					805:814	matrix components	798:814	matrix components (collagen and glycosaminoglycan content)	798:855	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	2	73	theme	reduced	418:424	arg1	immunogenicity					433:446	significantly reduced matrix immunogenicity	404:446	significantly reduced matrix immunogenicity	404:446	Recently it was shown that intensified detergent treatment completely removed cellular components from dEAC and thereby significantly reduced matrix immunogenicity.
25921001	0	74	theme	Decellularization	22:38	arg1	Effect					0:5	Effect	0:5	Effect of Intensified Decellularization of Equine Carotid Arteries on Scaffold Biomechanics and Cytotoxicity.	0:108	Effect of Intensified Decellularization of Equine Carotid Arteries on Scaffold Biomechanics and Cytotoxicity.
25921001	1	75	theme	Decellularized	110:123	arg1	dEAC					150:153	dEAC	150:153	dEAC	150:153	Decellularized equine carotid arteries (dEAC) are suggested to represent an alternative for alloplastic vascular grafts in haemodialysis patients to achieve vascular access.
25921001	1	75	theme	Decellularized	110:123	arg1	arteries					140:147	Decellularized equine carotid arteries	110:147	Decellularized equine carotid arteries (dEAC)	110:154	Decellularized equine carotid arteries (dEAC) are suggested to represent an alternative for alloplastic vascular grafts in haemodialysis patients to achieve vascular access.
25921001	0	76	theme	Equine	43:48	arg1	Arteries					58:65	Equine Carotid Arteries	43:65	Equine Carotid Arteries	43:65	Effect of Intensified Decellularization of Equine Carotid Arteries on Scaffold Biomechanics and Cytotoxicity.
25921001	7	77	theme	intact	1539:1544	arg1	lining					1558:1563	an intact endothelial lining	1536:1563	an intact endothelial lining after seeding	1536:1577	Cytotoxicity was not observed in either dEAC matrix which was also displayed by an intact endothelial lining after seeding.
25921001	4	78	theme	WST-8	895:899	arg1	assay					901:905	WST-8 assay	895:905	WST-8 assay	895:905	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	0	79	theme	Arteries	58:65	arg1	Decellularization					22:38	Intensified Decellularization	10:38	Intensified Decellularization of Equine Carotid Arteries	10:65	Effect of Intensified Decellularization of Equine Carotid Arteries on Scaffold Biomechanics and Cytotoxicity.
25921001	5	80	theme	32.2	1124:1127	arg1	n-EAC					1142:1146	n-EAC	1142:1146	n-EAC	1142:1146	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	80	theme	32.2	1124:1127	arg1	%					1137:1137	32.2 and 27.4%	1124:1137	%	1137:1137	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	81	theme	<	1151:1151	arg1	p					1149:1149	p < 0.01	1149:1156	p < 0.01	1149:1156	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	8	82	theme	tissue	1677:1682	arg1	purposes					1696:1703	vascular tissue engineering purposes	1668:1703	vascular tissue engineering purposes	1668:1703	Thus, even intensified decellularization generates matrix scaffolds highly suitable for vascular tissue engineering purposes, e.g., the generation of haemodialysis shunts.
25921001	5	83	theme	suture	1248:1253	arg1	strength					1265:1272	suture retention strength	1248:1272	suture retention strength	1248:1272	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	5	84	theme	venous	1096:1101	arg1	compliance					1112:1121	venous pressure compliance	1096:1121	venous pressure compliance	1096:1121	Both decellularization protocols comparably reduced matrix compliance (venous pressure compliance: 32.2 and 27.4% of n-EAC; p < 0.01 and arterial pressure compliance: 26.8 and 23.7% of n-EAC, p < 0.01) but had no effect on suture retention strength and burst pressure.
25921001	8	85	theme	matrix	1631:1636	arg1	scaffolds					1638:1646	matrix scaffolds	1631:1646	matrix scaffolds	1631:1646	Thus, even intensified decellularization generates matrix scaffolds highly suitable for vascular tissue engineering purposes, e.g., the generation of haemodialysis shunts.
25921001	4	86	from	pressure	787:794	arg1	strength					685:692	suture retention strength	668:692	suture retention strength	668:692	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	86	from	pressure	787:794	arg1	pressure					701:708	burst pressure	695:708	burst pressure	695:708	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	86	from	pressure	787:794	arg1	compliance					730:739	circumferential compliance	714:739	circumferential compliance	714:739	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25921001	4	86	from	pressure	787:794	arg1	characteristics					651:665	their biomechanical characteristics	631:665	their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure)	631:795	Therefore, intensively decellularized carotids (int-dEAC) were now evaluated for their biomechanical characteristics (suture retention strength, burst pressure and circumferential compliance at arterial and venous systolic and diastolic pressure), matrix components (collagen and glycosaminoglycan content) and indirect and direct cytotoxicity (WST-8 assay and endothelial cell seeding) and compared with native (n-EAC) and conventionally decellularized carotids (con-dEAC).
25523876	4	0	theme	PEGdA	1124:1128	arg1	concentration					1130:1142	PEGdA concentration	1124:1142	PEGdA concentration	1124:1142	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	3	1	theme	semi-IPN	744:751	arg1	composition					761:771	PEGdA/HA semi-IPN network composition	735:771	PEGdA/HA semi-IPN network composition	735:771	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	2	2	theme	polymer	396:402	arg1	networks					404:411	hybrid semi-interpenetrating polymer networks	367:411	hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA)	367:555	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	2	theme	polymer	396:402	arg1	semi-IPNs					414:422	semi-IPNs	414:422	semi-IPNs	414:422	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	8	3	theme	degradation	1759:1769	arg1	rate					1771:1774	the degradation rate	1755:1774	the degradation rate of the PEGdA component	1755:1797	In order to support long-term cellular remodeling, the degradation rate of the PEGdA component was optimized by preparing blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation.
25523876	10	4	theme	dynamic	2135:2141	arg1	microenvironments					2143:2159	dynamic microenvironments	2135:2159	dynamic microenvironments that can support 3-D cell survival, spreading and migration for a variety of cell therapy applications	2135:2262	These results demonstrate that PEGdA/HA semi-IPNs provide dynamic microenvironments that can support 3-D cell survival, spreading and migration for a variety of cell therapy applications.
25523876	1	5	theme	cell	306:309	arg1	spreading					311:319	cell spreading	306:319	cell spreading	306:319	To serve as artificial matrices for therapeutic cell transplantation, synthetic hydrogels must incorporate mechanisms enabling localized, cell-mediated degradation that allows cell spreading and migration.
25523876	9	6	theme	encapsulation	2056:2068	arg1	model					2070:2074	a gel-within-gel encapsulation model	2039:2074	a gel-within-gel encapsulation model	2039:2074	Optimized semi-IPN formulations supported long-term survival of encapsulated fibroblasts and sustained migration in a gel-within-gel encapsulation model.
25523876	3	7	theme	3-D	776:778	arg1	spreading					780:788	3-D spreading	776:788	3-D spreading of encapsulated fibroblasts	776:816	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	2	8	theme	hyaluronidase	657:669	arg1	activity					671:678	cellular hyaluronidase activity	648:678	cellular hyaluronidase activity	648:678	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	8	9	theme	PEGdA	1852:1856	arg1	macromers					1858:1866	three different PEGdA macromers	1836:1866	three different PEGdA macromers with varying susceptibility to hydrolytic degradation	1836:1920	In order to support long-term cellular remodeling, the degradation rate of the PEGdA component was optimized by preparing blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation.
25523876	1	10	theme	synthetic	200:208	arg1	hydrogels					210:218	synthetic hydrogels	200:218	synthetic hydrogels	200:218	To serve as artificial matrices for therapeutic cell transplantation, synthetic hydrogels must incorporate mechanisms enabling localized, cell-mediated degradation that allows cell spreading and migration.
25523876	2	11	theme	relative	590:597	arg1	spreading					580:588	increased cell spreading	565:588	increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity	565:678	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	3	12	theme	fibroblasts	806:816	arg1	spreading					780:788	3-D spreading	776:788	3-D spreading of encapsulated fibroblasts	776:816	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	6	13	theme	polymerization-induced	1420:1441	arg1	separation					1449:1458	polymerization-induced phase separation	1420:1458	polymerization-induced phase separation	1420:1458	This suggests that there is an underlying mechanism of polymerization-induced phase separation that results in HA-enriched defects within the network structure.
25523876	2	14	theme	cell	575:578	arg1	spreading					580:588	increased cell spreading	565:588	increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity	565:678	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	4	15	theme	time	1199:1202	arg1	points					1204:1209	early time points	1193:1209	early time points	1193:1209	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	3	16	theme	gel	845:847	arg1	structure					849:857	gel structure	845:857	gel structure responsible for this activity	845:887	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	1	17	dep	localized	257:265	arg1	cell-mediated					268:280	cell-mediated	268:280	cell-mediated	268:280	To serve as artificial matrices for therapeutic cell transplantation, synthetic hydrogels must incorporate mechanisms enabling localized, cell-mediated degradation that allows cell spreading and migration.
25523876	10	18	theme	cell	2182:2185	arg1	survival					2187:2194	3-D cell survival	2178:2194	3-D cell survival	2178:2194	These results demonstrate that PEGdA/HA semi-IPNs provide dynamic microenvironments that can support 3-D cell survival, spreading and migration for a variety of cell therapy applications.
25523876	8	19	theme	hydrolytic	1899:1908	arg1	degradation					1910:1920	hydrolytic degradation	1899:1920	hydrolytic degradation	1899:1920	In order to support long-term cellular remodeling, the degradation rate of the PEGdA component was optimized by preparing blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation.
25523876	3	20	theme	gel	919:921	arg1	formulations					923:934	optimized gel formulations	909:934	optimized gel formulations	909:934	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	3	21	theme	responsible	859:869	arg1	structure					849:857	gel structure	845:857	gel structure responsible for this activity	845:887	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	0	22	theme	network	71:77	arg1	compositions					79:90	acid semi-interpenetrating network compositions	44:90	acid semi-interpenetrating network compositions for 3-D cell spreading and migration	44:127	Poly(ethylene glycol) diacrylate/hyaluronic acid semi-interpenetrating network compositions for 3-D cell spreading and migration.
25523876	3	23	theme	long-term	947:955	arg1	survival					962:969	long-term cell survival	947:969	long-term cell survival	947:969	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	2	24	theme	native	529:534	arg1	diacrylates					485:495	hydrolytically degradable poly(ethylene glycol) diacrylates	437:495	hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA)	437:503	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	24	theme	native	529:534	arg1	HA					553:554	HA	553:554	HA	553:554	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	24	theme	native	529:534	arg1	acid					547:550	native hyaluronic acid	529:550	native hyaluronic acid (HA)	529:555	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	3	25	from	changes	834:840	arg1	spreading					780:788	3-D spreading	776:788	3-D spreading of encapsulated fibroblasts	776:816	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	3	25	from	changes	834:840	arg1	structure					849:857	gel structure	845:857	gel structure responsible for this activity	845:887	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	10	26	theme	cell	2238:2241	arg1	applications					2251:2262	cell therapy applications	2238:2262	cell therapy applications	2238:2262	These results demonstrate that PEGdA/HA semi-IPNs provide dynamic microenvironments that can support 3-D cell survival, spreading and migration for a variety of cell therapy applications.
25523876	6	27	theme	HA-enriched	1476:1486	arg1	defects					1488:1494	HA-enriched defects	1476:1494	HA-enriched defects within the network structure	1476:1523	This suggests that there is an underlying mechanism of polymerization-induced phase separation that results in HA-enriched defects within the network structure.
25523876	5	28	theme	gel	1222:1224	arg1	turbidity					1226:1234	Increased gel turbidity	1212:1234	Increased gel turbidity	1212:1234	Increased gel turbidity was observed in semi-IPNs, but not in copolymerized hydrogels containing methacrylated HA, which did not support cell spreading.
25523876	1	29	theme	therapeutic	166:176	arg1	transplantation					183:197	therapeutic cell transplantation	166:197	therapeutic cell transplantation	166:197	To serve as artificial matrices for therapeutic cell transplantation, synthetic hydrogels must incorporate mechanisms enabling localized, cell-mediated degradation that allows cell spreading and migration.
25523876	7	30	theme	hybrid	1686:1691	arg1	hydrogels					1693:1701	alternative hybrid hydrogels	1674:1701	alternative hybrid hydrogels	1674:1701	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	0	31	theme	cell	100:103	arg1	spreading					105:113	3-D cell spreading	96:113	3-D cell spreading	96:113	Poly(ethylene glycol) diacrylate/hyaluronic acid semi-interpenetrating network compositions for 3-D cell spreading and migration.
25523876	4	32	theme	HA	1040:1041	arg1	concentration					1043:1055	HA concentration	1040:1055	HA concentration	1040:1055	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	5	33	theme	methacrylated	1309:1321	arg1	HA					1323:1324	methacrylated HA	1309:1324	methacrylated HA	1309:1324	Increased gel turbidity was observed in semi-IPNs, but not in copolymerized hydrogels containing methacrylated HA, which did not support cell spreading.
25523876	9	34	theme	long-term	1965:1973	arg1	survival					1975:1982	long-term survival	1965:1982	long-term survival of encapsulated fibroblasts	1965:2010	Optimized semi-IPN formulations supported long-term survival of encapsulated fibroblasts and sustained migration in a gel-within-gel encapsulation model.
25523876	2	35	theme	ethylene	468:475	arg1	poly					463:466	hydrolytically degradable poly	437:466	hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA)	437:503	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	35	theme	ethylene	468:475	arg1	glycol					477:482	ethylene glycol	468:482	ethylene glycol	468:482	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	0	36	theme	ethylene	5:12	arg1	Poly					0:3	Poly	0:3	Poly(ethylene glycol)	0:20	Poly(ethylene glycol) diacrylate/hyaluronic acid semi-interpenetrating network compositions for 3-D cell spreading and migration.
25523876	0	36	theme	ethylene	5:12	arg1	glycol					14:19	ethylene glycol	5:19	ethylene glycol	5:19	Poly(ethylene glycol) diacrylate/hyaluronic acid semi-interpenetrating network compositions for 3-D cell spreading and migration.
25523876	4	37	theme	HA	1077:1078	arg1	weight					1090:1095	a minimum HA molecular weight	1067:1095	a minimum HA molecular weight	1067:1095	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	2	38	theme	poly	463:466	arg1	diacrylates					485:495	hydrolytically degradable poly(ethylene glycol) diacrylates	437:495	hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA)	437:503	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	38	theme	poly	463:466	arg1	acid					547:550	native hyaluronic acid	529:550	native hyaluronic acid (HA)	529:555	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	38	theme	poly	463:466	arg1	PEGdA					498:502	PEGdA	498:502	PEGdA	498:502	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	38	theme	poly	463:466	arg1	acrylate-PEG-GRGDS					506:523	acrylate-PEG-GRGDS	506:523	acrylate-PEG-GRGDS	506:523	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	9	39	theme	encapsulated	1987:1998	arg1	fibroblasts					2000:2010	encapsulated fibroblasts	1987:2010	encapsulated fibroblasts	1987:2010	Optimized semi-IPN formulations supported long-term survival of encapsulated fibroblasts and sustained migration in a gel-within-gel encapsulation model.
25523876	7	40	theme	∼10kPa	1638:1643	arg1	properties					1626:1635	mechanical properties	1615:1635	mechanical properties (∼10kPa elastic modulus)	1615:1660	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	7	40	theme	∼10kPa	1638:1643	arg1	modulus					1653:1659	∼10kPa elastic modulus	1638:1659	∼10kPa elastic modulus	1638:1659	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	9	41	theme	fibroblasts	2000:2010	arg1	survival					1975:1982	long-term survival	1965:1982	long-term survival of encapsulated fibroblasts	1965:2010	Optimized semi-IPN formulations supported long-term survival of encapsulated fibroblasts and sustained migration in a gel-within-gel encapsulation model.
25523876	2	42	theme	semi-interpenetrating	374:394	arg1	networks					404:411	hybrid semi-interpenetrating polymer networks	367:411	hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA)	367:555	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	42	theme	semi-interpenetrating	374:394	arg1	semi-IPNs					414:422	semi-IPNs	414:422	semi-IPNs	414:422	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	0	43	theme	acid	44:47	arg1	compositions					79:90	acid semi-interpenetrating network compositions	44:90	acid semi-interpenetrating network compositions for 3-D cell spreading and migration	44:127	Poly(ethylene glycol) diacrylate/hyaluronic acid semi-interpenetrating network compositions for 3-D cell spreading and migration.
25523876	7	44	theme	properties	1626:1635	arg1	levels					1605:1610	relatively high levels	1589:1610	relatively high levels of mechanical properties (∼10kPa elastic modulus)	1589:1660	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	3	45	theme	PEGdA/HA	735:742	arg1	composition					761:771	PEGdA/HA semi-IPN network composition	735:771	PEGdA/HA semi-IPN network composition	735:771	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	4	46	theme	degradation	1178:1188	arg1	independent					1152:1162	independent	1152:1162	independent	1152:1162	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	2	47	theme	degradable	452:461	arg1	poly					463:466	hydrolytically degradable poly	437:466	hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA)	437:503	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	47	theme	degradable	452:461	arg1	glycol					477:482	ethylene glycol	468:482	ethylene glycol	468:482	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	7	48	theme	high	1600:1603	arg1	levels					1605:1610	relatively high levels	1589:1610	relatively high levels of mechanical properties (∼10kPa elastic modulus)	1589:1660	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	9	49	theme	gel-within-gel	2041:2054	arg1	model					2070:2074	a gel-within-gel encapsulation model	2039:2074	a gel-within-gel encapsulation model	2039:2074	Optimized semi-IPN formulations supported long-term survival of encapsulated fibroblasts and sustained migration in a gel-within-gel encapsulation model.
25523876	8	50	theme	long-term	1724:1732	arg1	remodeling					1743:1752	long-term cellular remodeling	1724:1752	long-term cellular remodeling	1724:1752	In order to support long-term cellular remodeling, the degradation rate of the PEGdA component was optimized by preparing blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation.
25523876	8	51	theme	component	1789:1797	arg1	rate					1771:1774	the degradation rate	1755:1774	the degradation rate of the PEGdA component	1755:1797	In order to support long-term cellular remodeling, the degradation rate of the PEGdA component was optimized by preparing blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation.
25523876	5	52	theme	copolymerized	1274:1286	arg1	hydrogels					1288:1296	copolymerized hydrogels	1274:1296	copolymerized hydrogels containing methacrylated HA, which did not support cell spreading	1274:1362	Increased gel turbidity was observed in semi-IPNs, but not in copolymerized hydrogels containing methacrylated HA, which did not support cell spreading.
25523876	3	53	theme	network	753:759	arg1	composition					761:771	PEGdA/HA semi-IPN network composition	735:771	PEGdA/HA semi-IPN network composition	735:771	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	7	54	theme	cell	1571:1574	arg1	spreading					1576:1584	cell spreading	1571:1584	cell spreading	1571:1584	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	3	55	theme	encapsulated	793:804	arg1	fibroblasts					806:816	encapsulated fibroblasts	793:816	encapsulated fibroblasts	793:816	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	1	56	theme	cell	178:181	arg1	transplantation					183:197	therapeutic cell transplantation	166:197	therapeutic cell transplantation	166:197	To serve as artificial matrices for therapeutic cell transplantation, synthetic hydrogels must incorporate mechanisms enabling localized, cell-mediated degradation that allows cell spreading and migration.
25523876	6	57	theme	network	1507:1513	arg1	structure					1515:1523	the network structure	1503:1523	the network structure	1503:1523	This suggests that there is an underlying mechanism of polymerization-induced phase separation that results in HA-enriched defects within the network structure.
25523876	5	58	contain	containing	1298:1307	arg1	hydrogels					1288:1296	copolymerized hydrogels	1274:1296	copolymerized hydrogels containing methacrylated HA, which did not support cell spreading	1274:1362	Increased gel turbidity was observed in semi-IPNs, but not in copolymerized hydrogels containing methacrylated HA, which did not support cell spreading.
25523876	5	58	contain	containing	1298:1307	arg2	HA					1323:1324	methacrylated HA	1309:1324	methacrylated HA	1309:1324	Increased gel turbidity was observed in semi-IPNs, but not in copolymerized hydrogels containing methacrylated HA, which did not support cell spreading.
25523876	10	59	theme	PEGdA/HA	2108:2115	arg1	semi-IPNs					2117:2125	PEGdA/HA semi-IPNs	2108:2125	PEGdA/HA semi-IPNs	2108:2125	These results demonstrate that PEGdA/HA semi-IPNs provide dynamic microenvironments that can support 3-D cell survival, spreading and migration for a variety of cell therapy applications.
25523876	2	60	theme	cellular	648:655	arg1	activity					671:678	cellular hyaluronidase activity	648:678	cellular hyaluronidase activity	648:678	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	7	61	theme	PEGdA/HA	1526:1533	arg1	semi-IPNs					1535:1543	PEGdA/HA semi-IPNs	1526:1543	PEGdA/HA semi-IPNs	1526:1543	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	4	62	theme	early	1193:1197	arg1	points					1204:1209	early time points	1193:1209	early time points	1193:1209	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	3	63	theme	underlying	823:832	arg1	changes					834:840	the underlying changes	819:840	the underlying changes in gel structure responsible for this activity	819:887	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	6	64	theme	phase	1443:1447	arg1	separation					1449:1458	polymerization-induced phase separation	1420:1458	polymerization-induced phase separation	1420:1458	This suggests that there is an underlying mechanism of polymerization-induced phase separation that results in HA-enriched defects within the network structure.
25523876	8	65	theme	different	1842:1850	arg1	macromers					1858:1866	three different PEGdA macromers	1836:1866	three different PEGdA macromers with varying susceptibility to hydrolytic degradation	1836:1920	In order to support long-term cellular remodeling, the degradation rate of the PEGdA component was optimized by preparing blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation.
25523876	4	66	from	points	1204:1209	arg1	independent					1152:1162	independent	1152:1162	independent	1152:1162	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	4	67	theme	Fibroblast	986:995	arg1	spreading					997:1005	Fibroblast spreading	986:1005	Fibroblast spreading	986:1005	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	8	68	theme	macromers	1858:1866	arg1	blends					1826:1831	blends	1826:1831	blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation	1826:1920	In order to support long-term cellular remodeling, the degradation rate of the PEGdA component was optimized by preparing blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation.
25523876	2	69	theme	increased	565:573	arg1	spreading					580:588	increased cell spreading	565:588	increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity	565:678	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	6	70	theme	underlying	1396:1405	arg1	mechanism					1407:1415	an underlying mechanism	1393:1415	an underlying mechanism of polymerization-induced phase separation that results in HA-enriched defects within the network structure	1393:1523	This suggests that there is an underlying mechanism of polymerization-induced phase separation that results in HA-enriched defects within the network structure.
25523876	8	71	theme	varying	1873:1879	arg1	susceptibility					1881:1894	varying susceptibility	1873:1894	varying susceptibility to hydrolytic degradation	1873:1920	In order to support long-term cellular remodeling, the degradation rate of the PEGdA component was optimized by preparing blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation.
25523876	2	72	theme	synthetic	608:616	arg1	networks					618:625	fully synthetic networks	602:625	fully synthetic networks that is dependent on cellular hyaluronidase activity	602:678	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	10	73	theme	3-D	2178:2180	arg1	survival					2187:2194	3-D cell survival	2178:2194	3-D cell survival	2178:2194	These results demonstrate that PEGdA/HA semi-IPNs provide dynamic microenvironments that can support 3-D cell survival, spreading and migration for a variety of cell therapy applications.
25523876	3	74	theme	formulations	923:934	arg1	effects					724:730	the effects	720:730	the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts	720:816	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	3	74	theme	formulations	923:934	arg1	changes					834:840	the underlying changes	819:840	the underlying changes in gel structure responsible for this activity	819:887	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	3	74	theme	formulations	923:934	arg1	ability					898:904	the ability	894:904	the ability of optimized gel formulations to support long-term cell survival and migration	894:983	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	1	75	theme	artificial	142:151	arg1	matrices					153:160	artificial matrices	142:160	artificial matrices for therapeutic cell transplantation	142:197	To serve as artificial matrices for therapeutic cell transplantation, synthetic hydrogels must incorporate mechanisms enabling localized, cell-mediated degradation that allows cell spreading and migration.
25523876	3	76	theme	optimized	909:917	arg1	formulations					923:934	optimized gel formulations	909:934	optimized gel formulations	909:934	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	2	77	theme	hyaluronic	536:545	arg1	diacrylates					485:495	hydrolytically degradable poly(ethylene glycol) diacrylates	437:495	hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA)	437:503	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	77	theme	hyaluronic	536:545	arg1	HA					553:554	HA	553:554	HA	553:554	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	77	theme	hyaluronic	536:545	arg1	acid					547:550	native hyaluronic acid	529:550	native hyaluronic acid (HA)	529:555	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	0	78	theme	3-D	96:98	arg1	spreading					105:113	3-D cell spreading	96:113	3-D cell spreading	96:113	Poly(ethylene glycol) diacrylate/hyaluronic acid semi-interpenetrating network compositions for 3-D cell spreading and migration.
25523876	5	79	theme	Increased	1212:1220	arg1	turbidity					1226:1234	Increased gel turbidity	1212:1234	Increased gel turbidity	1212:1234	Increased gel turbidity was observed in semi-IPNs, but not in copolymerized hydrogels containing methacrylated HA, which did not support cell spreading.
25523876	3	80	theme	cell	957:960	arg1	survival					962:969	long-term cell survival	947:969	long-term cell survival	947:969	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	7	81	theme	alternative	1674:1684	arg1	hydrogels					1693:1701	alternative hybrid hydrogels	1674:1701	alternative hybrid hydrogels	1674:1701	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	5	82	theme	cell	1349:1352	arg1	spreading					1354:1362	cell spreading	1349:1362	cell spreading	1349:1362	Increased gel turbidity was observed in semi-IPNs, but not in copolymerized hydrogels containing methacrylated HA, which did not support cell spreading.
25523876	4	83	theme	biphasic	1019:1026	arg1	response					1028:1035	a biphasic response	1017:1035	a biphasic response to HA concentration	1017:1055	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	10	84	theme	applications	2251:2262	arg1	variety					2227:2233	a variety	2225:2233	a variety of cell therapy applications	2225:2262	These results demonstrate that PEGdA/HA semi-IPNs provide dynamic microenvironments that can support 3-D cell survival, spreading and migration for a variety of cell therapy applications.
25523876	10	84	theme	applications	2251:2262	arg1	applications					2251:2262	cell therapy applications	2238:2262	cell therapy applications	2238:2262	These results demonstrate that PEGdA/HA semi-IPNs provide dynamic microenvironments that can support 3-D cell survival, spreading and migration for a variety of cell therapy applications.
25523876	9	85	theme	semi-IPN	1933:1940	arg1	formulations					1942:1953	Optimized semi-IPN formulations	1923:1953	Optimized semi-IPN formulations	1923:1953	Optimized semi-IPN formulations supported long-term survival of encapsulated fibroblasts and sustained migration in a gel-within-gel encapsulation model.
25523876	8	86	with	macromers	1858:1866	arg1	susceptibility					1881:1894	varying susceptibility	1873:1894	varying susceptibility to hydrolytic degradation	1873:1920	In order to support long-term cellular remodeling, the degradation rate of the PEGdA component was optimized by preparing blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation.
25523876	9	87	theme	Optimized	1923:1931	arg1	formulations					1942:1953	Optimized semi-IPN formulations	1923:1953	Optimized semi-IPN formulations	1923:1953	Optimized semi-IPN formulations supported long-term survival of encapsulated fibroblasts and sustained migration in a gel-within-gel encapsulation model.
25523876	5	88	located	observed	1240:1247	arg2	turbidity					1226:1234	Increased gel turbidity	1212:1234	Increased gel turbidity	1212:1234	Increased gel turbidity was observed in semi-IPNs, but not in copolymerized hydrogels containing methacrylated HA, which did not support cell spreading.
25523876	5	88	located	observed	1240:1247	arg1	semi-IPNs					1252:1260	semi-IPNs	1252:1260	semi-IPNs	1252:1260	Increased gel turbidity was observed in semi-IPNs, but not in copolymerized hydrogels containing methacrylated HA, which did not support cell spreading.
25523876	10	89	theme	therapy	2243:2249	arg1	applications					2251:2262	cell therapy applications	2238:2262	cell therapy applications	2238:2262	These results demonstrate that PEGdA/HA semi-IPNs provide dynamic microenvironments that can support 3-D cell survival, spreading and migration for a variety of cell therapy applications.
25523876	4	90	theme	minimum	1069:1075	arg1	weight					1090:1095	a minimum HA molecular weight	1067:1095	a minimum HA molecular weight	1067:1095	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	3	91	from	ability	898:904	arg1	spreading					780:788	3-D spreading	776:788	3-D spreading of encapsulated fibroblasts	776:816	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	3	91	from	ability	898:904	arg1	structure					849:857	gel structure	845:857	gel structure responsible for this activity	845:887	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	6	92	theme	separation	1449:1458	arg1	mechanism					1407:1415	an underlying mechanism	1393:1415	an underlying mechanism of polymerization-induced phase separation that results in HA-enriched defects within the network structure	1393:1523	This suggests that there is an underlying mechanism of polymerization-induced phase separation that results in HA-enriched defects within the network structure.
25523876	0	93	theme	semi-interpenetrating	49:69	arg1	compositions					79:90	acid semi-interpenetrating network compositions	44:90	acid semi-interpenetrating network compositions for 3-D cell spreading and migration	44:127	Poly(ethylene glycol) diacrylate/hyaluronic acid semi-interpenetrating network compositions for 3-D cell spreading and migration.
25523876	7	94	theme	elastic	1645:1651	arg1	properties					1626:1635	mechanical properties	1615:1635	mechanical properties (∼10kPa elastic modulus)	1615:1660	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	7	94	theme	elastic	1645:1651	arg1	modulus					1653:1659	∼10kPa elastic modulus	1638:1659	∼10kPa elastic modulus	1638:1659	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	4	95	theme	molecular	1080:1088	arg1	weight					1090:1095	a minimum HA molecular weight	1067:1095	a minimum HA molecular weight	1067:1095	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	2	96	theme	hybrid	367:372	arg1	networks					404:411	hybrid semi-interpenetrating polymer networks	367:411	hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA)	367:555	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	2	96	theme	hybrid	367:372	arg1	semi-IPNs					414:422	semi-IPNs	414:422	semi-IPNs	414:422	Previously, we have shown that hybrid semi-interpenetrating polymer networks (semi-IPNs) composed of hydrolytically degradable poly(ethylene glycol) diacrylates (PEGdA), acrylate-PEG-GRGDS and native hyaluronic acid (HA) support increased cell spreading relative to fully synthetic networks that is dependent on cellular hyaluronidase activity.
25523876	8	97	theme	PEGdA	1783:1787	arg1	component					1789:1797	the PEGdA component	1779:1797	the PEGdA component	1779:1797	In order to support long-term cellular remodeling, the degradation rate of the PEGdA component was optimized by preparing blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation.
25523876	1	98	theme	localized	257:265	arg1	degradation					282:292	localized, cell-mediated degradation	257:292	localized, cell-mediated degradation that allows cell spreading and migration	257:333	To serve as artificial matrices for therapeutic cell transplantation, synthetic hydrogels must incorporate mechanisms enabling localized, cell-mediated degradation that allows cell spreading and migration.
25523876	7	99	theme	mechanical	1615:1624	arg1	properties					1626:1635	mechanical properties	1615:1635	mechanical properties (∼10kPa elastic modulus)	1615:1660	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	7	99	theme	mechanical	1615:1624	arg1	modulus					1653:1659	∼10kPa elastic modulus	1638:1659	∼10kPa elastic modulus	1638:1659	PEGdA/HA semi-IPNs were also able to support cell spreading at relatively high levels of mechanical properties (∼10kPa elastic modulus) compared to alternative hybrid hydrogels.
25523876	4	100	theme	hydrolytic	1167:1176	arg1	degradation					1178:1188	hydrolytic degradation	1167:1188	hydrolytic degradation	1167:1188	Fibroblast spreading exhibited a biphasic response to HA concentration, required a minimum HA molecular weight, decreased with increasing PEGdA concentration and was independent of hydrolytic degradation at early time points.
25523876	8	101	theme	cellular	1734:1741	arg1	remodeling					1743:1752	long-term cellular remodeling	1724:1752	long-term cellular remodeling	1724:1752	In order to support long-term cellular remodeling, the degradation rate of the PEGdA component was optimized by preparing blends of three different PEGdA macromers with varying susceptibility to hydrolytic degradation.
25523876	3	102	from	effects	724:730	arg1	spreading					780:788	3-D spreading	776:788	3-D spreading of encapsulated fibroblasts	776:816	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	3	102	from	effects	724:730	arg1	structure					849:857	gel structure	845:857	gel structure responsible for this activity	845:887	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	3	103	theme	composition	761:771	arg1	effects					724:730	the effects	720:730	the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts	720:816	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	3	103	theme	composition	761:771	arg1	changes					834:840	the underlying changes	819:840	the underlying changes in gel structure responsible for this activity	819:887	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
25523876	3	103	theme	composition	761:771	arg1	ability					898:904	the ability	894:904	the ability of optimized gel formulations to support long-term cell survival and migration	894:983	This study systematically investigated the effects of PEGdA/HA semi-IPN network composition on 3-D spreading of encapsulated fibroblasts, the underlying changes in gel structure responsible for this activity, and the ability of optimized gel formulations to support long-term cell survival and migration.
28528593	0	0	theme	high-sucrose	88:99	arg1	diet					101:104	a high-fat and high-sucrose diet	73:104	diet	101:104	Polymannuronic acid ameliorated obesity and inflammation associated with a high-fat and high-sucrose diet by modulating the gut microbiome in a murine model.
28528593	7	1	theme	bioactive	1169:1177	arg1	studies					1140:1146	mechanistic studies	1128:1146	mechanistic studies on PM	1128:1152	Our findings should facilitate mechanistic studies on PM as a potential bioactive compound to alleviate obesity and the metabolic syndrome.
28528593	7	1	theme	bioactive	1169:1177	arg1	compound					1179:1186	a potential bioactive compound	1157:1186	a potential bioactive compound to alleviate obesity and the metabolic syndrome	1157:1234	Our findings should facilitate mechanistic studies on PM as a potential bioactive compound to alleviate obesity and the metabolic syndrome.
28528593	2	2	theme	potential	315:323	arg1	role					325:328	its potential role	311:328	its potential role in reducing body weight gain and attenuating inflammation induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating the gut microbiome in mice	311:499	In this study, we examined its potential role in reducing body weight gain and attenuating inflammation induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating the gut microbiome in mice.
28528593	4	3	theme	gut	673:675	arg1	microbiome					677:686	the gut microbiome	669:686	the gut microbiome	669:686	PM also had a profound impact on the microbial composition in the gut microbiome and resulted in a distinct microbiome structure.
28528593	1	4	contain	possess	252:258	arg1	PM					179:180	PM	179:180	PM	179:180	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	1	4	contain	possess	252:258	arg1	one					184:186	one	184:186	one	184:186	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	1	4	contain	possess	252:258	arg1	acid					173:176	Polymannuronic acid	158:176	Polymannuronic acid (PM)	158:181	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	1	4	contain	possess	252:258	arg2	activities					272:281	antioxidant activities	260:281	antioxidant activities	260:281	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	1	4	contain	possess	252:258	arg1	alginates					200:208	numerous alginates	191:208	numerous alginates isolated from brown seaweeds	191:237	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	7	5	theme	mechanistic	1128:1138	arg1	studies					1140:1146	mechanistic studies	1128:1146	mechanistic studies on PM	1128:1152	Our findings should facilitate mechanistic studies on PM as a potential bioactive compound to alleviate obesity and the metabolic syndrome.
28528593	7	5	theme	mechanistic	1128:1138	arg1	compound					1179:1186	a potential bioactive compound	1157:1186	a potential bioactive compound to alleviate obesity and the metabolic syndrome	1157:1234	Our findings should facilitate mechanistic studies on PM as a potential bioactive compound to alleviate obesity and the metabolic syndrome.
28528593	5	6	theme	Lactobacillus	817:829	arg1	bacterium					806:814	a probiotic bacterium	794:814	a probiotic bacterium	794:814	For example, PM significantly increased the abundance of a probiotic bacterium, Lactobacillus reuteri (log10 LDA score>2·0).
28528593	6	7	theme	probiotic	990:998	arg1	activities					1000:1009	probiotic activities	990:1009	probiotic activities	990:1009	Together, our results suggest that PM may exert its immunoregulatory effects by enhancing proliferation of several species with probiotic activities while repressing the abundance of the microbial taxa that harbor potential pathogens.
28528593	2	8	theme	high-sucrose	414:425	arg1	HFD					433:435	HFD	433:435	HFD	433:435	In this study, we examined its potential role in reducing body weight gain and attenuating inflammation induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating the gut microbiome in mice.
28528593	2	8	theme	high-sucrose	414:425	arg1	diet					427:430	a high-fat and high-sucrose diet	399:430	diet	427:430	In this study, we examined its potential role in reducing body weight gain and attenuating inflammation induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating the gut microbiome in mice.
28528593	3	9	theme	30-d	504:507	arg1	treatment					512:520	A 30-d PM treatment	502:520	A 30-d PM treatment	502:520	A 30-d PM treatment significantly reduced the diet-induced body weight gain and blood TAG levels (P2·0).
28528593	2	10	theme	weight	347:352	arg1	gain					354:357	body weight gain	342:357	body weight gain	342:357	In this study, we examined its potential role in reducing body weight gain and attenuating inflammation induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating the gut microbiome in mice.
28528593	0	11	theme	Polymannuronic	0:13	arg1	acid					15:18	Polymannuronic acid	0:18	Polymannuronic acid	0:18	Polymannuronic acid ameliorated obesity and inflammation associated with a high-fat and high-sucrose diet by modulating the gut microbiome in a murine model.
28528593	4	12	from	composition	654:664	arg1	microbiome					677:686	the gut microbiome	669:686	the gut microbiome	669:686	PM also had a profound impact on the microbial composition in the gut microbiome and resulted in a distinct microbiome structure.
28528593	3	13	theme	PM	509:510	arg1	treatment					512:520	A 30-d PM treatment	502:520	A 30-d PM treatment	502:520	A 30-d PM treatment significantly reduced the diet-induced body weight gain and blood TAG levels (P2·0).
28528593	2	14	theme	body	342:345	arg1	gain					354:357	body weight gain	342:357	body weight gain	342:357	In this study, we examined its potential role in reducing body weight gain and attenuating inflammation induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating the gut microbiome in mice.
28528593	2	15	theme	high-fat	401:408	arg1	HFD					433:435	HFD	433:435	HFD	433:435	In this study, we examined its potential role in reducing body weight gain and attenuating inflammation induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating the gut microbiome in mice.
28528593	2	15	theme	high-fat	401:408	arg1	diet					427:430	a high-fat and high-sucrose diet	399:430	diet	427:430	In this study, we examined its potential role in reducing body weight gain and attenuating inflammation induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating the gut microbiome in mice.
28528593	4	16	theme	microbiome	715:724	arg1	structure					726:734	a distinct microbiome structure	704:734	a distinct microbiome structure	704:734	PM also had a profound impact on the microbial composition in the gut microbiome and resulted in a distinct microbiome structure.
28528593	6	17	theme	several	969:975	arg1	species					977:983	several species	969:983	several species with probiotic activities	969:1009	Together, our results suggest that PM may exert its immunoregulatory effects by enhancing proliferation of several species with probiotic activities while repressing the abundance of the microbial taxa that harbor potential pathogens.
28528593	4	18	theme	microbial	644:652	arg1	composition					654:664	the microbial composition	640:664	the microbial composition in the gut microbiome	640:686	PM also had a profound impact on the microbial composition in the gut microbiome and resulted in a distinct microbiome structure.
28528593	1	19	theme	antioxidant	260:270	arg1	activities					272:281	antioxidant activities	260:281	antioxidant activities	260:281	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	0	20	theme	gut	124:126	arg1	microbiome					128:137	the gut microbiome	120:137	the gut microbiome	120:137	Polymannuronic acid ameliorated obesity and inflammation associated with a high-fat and high-sucrose diet by modulating the gut microbiome in a murine model.
28528593	3	21	theme	TAG	588:590	arg1	P2·0					600:603	P2·0	600:603	P2·0	600:603	A 30-d PM treatment significantly reduced the diet-induced body weight gain and blood TAG levels (P2·0).
28528593	3	21	theme	TAG	588:590	arg1	levels					592:597	blood TAG levels	582:597	blood TAG levels (P2·0)	582:604	A 30-d PM treatment significantly reduced the diet-induced body weight gain and blood TAG levels (P2·0).
28528593	1	22	theme	Polymannuronic	158:171	arg1	PM					179:180	PM	179:180	PM	179:180	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	1	22	theme	Polymannuronic	158:171	arg1	one					184:186	one	184:186	one	184:186	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	1	22	theme	Polymannuronic	158:171	arg1	acid					173:176	Polymannuronic acid	158:176	Polymannuronic acid (PM)	158:181	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	1	22	theme	Polymannuronic	158:171	arg1	alginates					200:208	numerous alginates	191:208	numerous alginates isolated from brown seaweeds	191:237	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	6	23	theme	potential	1076:1084	arg1	pathogens					1086:1094	potential pathogens	1076:1094	potential pathogens	1076:1094	Together, our results suggest that PM may exert its immunoregulatory effects by enhancing proliferation of several species with probiotic activities while repressing the abundance of the microbial taxa that harbor potential pathogens.
28528593	6	24	theme	microbial	1049:1057	arg1	taxa					1059:1062	the microbial taxa	1045:1062	the microbial taxa that harbor potential pathogens	1045:1094	Together, our results suggest that PM may exert its immunoregulatory effects by enhancing proliferation of several species with probiotic activities while repressing the abundance of the microbial taxa that harbor potential pathogens.
28528593	6	25	with	species	977:983	arg1	activities					1000:1009	probiotic activities	990:1009	probiotic activities	990:1009	Together, our results suggest that PM may exert its immunoregulatory effects by enhancing proliferation of several species with probiotic activities while repressing the abundance of the microbial taxa that harbor potential pathogens.
28528593	1	26	attach	isolated	210:217	arg2	alginates					200:208	numerous alginates	191:208	numerous alginates isolated from brown seaweeds	191:237	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	1	26	attach	isolated	210:217	arg1	seaweeds					230:237	brown seaweeds	224:237	brown seaweeds	224:237	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	6	27	theme	species	977:983	arg1	proliferation					952:964	proliferation	952:964	proliferation of several species with probiotic activities	952:1009	Together, our results suggest that PM may exert its immunoregulatory effects by enhancing proliferation of several species with probiotic activities while repressing the abundance of the microbial taxa that harbor potential pathogens.
28528593	2	28	theme	gut	478:480	arg1	microbiome					482:491	the gut microbiome	474:491	the gut microbiome in mice	474:499	In this study, we examined its potential role in reducing body weight gain and attenuating inflammation induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating the gut microbiome in mice.
28528593	7	29	from	studies	1140:1146	arg1	PM					1151:1152	PM	1151:1152	PM	1151:1152	Our findings should facilitate mechanistic studies on PM as a potential bioactive compound to alleviate obesity and the metabolic syndrome.
28528593	1	30	theme	numerous	191:198	arg1	alginates					200:208	numerous alginates	191:208	numerous alginates isolated from brown seaweeds	191:237	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	3	31	theme	blood	582:586	arg1	P2·0					600:603	P2·0	600:603	P2·0	600:603	A 30-d PM treatment significantly reduced the diet-induced body weight gain and blood TAG levels (P2·0).
28528593	3	31	theme	blood	582:586	arg1	levels					592:597	blood TAG levels	582:597	blood TAG levels (P2·0)	582:604	A 30-d PM treatment significantly reduced the diet-induced body weight gain and blood TAG levels (P2·0).
28528593	5	32	dep	Lactobacillus	817:829	arg1	reuteri					831:837	reuteri	831:837	reuteri	831:837	For example, PM significantly increased the abundance of a probiotic bacterium, Lactobacillus reuteri (log10 LDA score>2·0).
28528593	6	33	theme	taxa	1059:1062	arg1	abundance					1032:1040	the abundance	1028:1040	the abundance of the microbial taxa that harbor potential pathogens	1028:1094	Together, our results suggest that PM may exert its immunoregulatory effects by enhancing proliferation of several species with probiotic activities while repressing the abundance of the microbial taxa that harbor potential pathogens.
28528593	0	34	theme	murine	144:149	arg1	model					151:155	a murine model	142:155	a murine model	142:155	Polymannuronic acid ameliorated obesity and inflammation associated with a high-fat and high-sucrose diet by modulating the gut microbiome in a murine model.
28528593	4	35	theme	profound	621:628	arg1	impact					630:635	a profound impact	619:635	a profound impact	619:635	PM also had a profound impact on the microbial composition in the gut microbiome and resulted in a distinct microbiome structure.
28528593	4	36	theme	distinct	706:713	arg1	structure					726:734	a distinct microbiome structure	704:734	a distinct microbiome structure	704:734	PM also had a profound impact on the microbial composition in the gut microbiome and resulted in a distinct microbiome structure.
28528593	3	37	theme	weight	566:571	arg1	gain					573:576	the diet-induced body weight gain	544:576	the diet-induced body weight gain	544:576	A 30-d PM treatment significantly reduced the diet-induced body weight gain and blood TAG levels (P2·0).
28528593	5	38	theme	log10	840:844	arg1	LDA					846:848	log10 LDA score>2·0	840:858	log10 LDA score>2·0	840:858	For example, PM significantly increased the abundance of a probiotic bacterium, Lactobacillus reuteri (log10 LDA score>2·0).
28528593	2	39	from	microbiome	482:491	arg1	mice					496:499	mice	496:499	mice	496:499	In this study, we examined its potential role in reducing body weight gain and attenuating inflammation induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating the gut microbiome in mice.
28528593	7	40	theme	metabolic	1217:1225	arg1	syndrome					1227:1234	the metabolic syndrome	1213:1234	the metabolic syndrome	1213:1234	Our findings should facilitate mechanistic studies on PM as a potential bioactive compound to alleviate obesity and the metabolic syndrome.
28528593	5	41	dep	increased	767:775	arg1	LDA					846:848	log10 LDA score>2·0	840:858	log10 LDA score>2·0	840:858	For example, PM significantly increased the abundance of a probiotic bacterium, Lactobacillus reuteri (log10 LDA score>2·0).
28528593	5	42	theme	bacterium	806:814	arg1	abundance					781:789	the abundance	777:789	the abundance of a probiotic bacterium, Lactobacillus reuteri	777:837	For example, PM significantly increased the abundance of a probiotic bacterium, Lactobacillus reuteri (log10 LDA score>2·0).
28528593	0	43	theme	high-fat	75:82	arg1	diet					101:104	a high-fat and high-sucrose diet	73:104	diet	101:104	Polymannuronic acid ameliorated obesity and inflammation associated with a high-fat and high-sucrose diet by modulating the gut microbiome in a murine model.
28528593	3	44	theme	diet-induced	548:559	arg1	gain					573:576	the diet-induced body weight gain	544:576	the diet-induced body weight gain	544:576	A 30-d PM treatment significantly reduced the diet-induced body weight gain and blood TAG levels (P2·0).
28528593	5	45	theme	probiotic	796:804	arg1	bacterium					806:814	a probiotic bacterium	794:814	a probiotic bacterium	794:814	For example, PM significantly increased the abundance of a probiotic bacterium, Lactobacillus reuteri (log10 LDA score>2·0).
28528593	6	46	theme	immunoregulatory	914:929	arg1	effects					931:937	its immunoregulatory effects	910:937	its immunoregulatory effects	910:937	Together, our results suggest that PM may exert its immunoregulatory effects by enhancing proliferation of several species with probiotic activities while repressing the abundance of the microbial taxa that harbor potential pathogens.
28528593	7	47	theme	potential	1159:1167	arg1	studies					1140:1146	mechanistic studies	1128:1146	mechanistic studies on PM	1128:1152	Our findings should facilitate mechanistic studies on PM as a potential bioactive compound to alleviate obesity and the metabolic syndrome.
28528593	7	47	theme	potential	1159:1167	arg1	compound					1179:1186	a potential bioactive compound	1157:1186	a potential bioactive compound to alleviate obesity and the metabolic syndrome	1157:1234	Our findings should facilitate mechanistic studies on PM as a potential bioactive compound to alleviate obesity and the metabolic syndrome.
28528593	4	48	contain	had	615:617	arg1	PM					607:608	PM	607:608	PM	607:608	PM also had a profound impact on the microbial composition in the gut microbiome and resulted in a distinct microbiome structure.
28528593	4	48	contain	had	615:617	arg2	impact					630:635	a profound impact	619:635	a profound impact	619:635	PM also had a profound impact on the microbial composition in the gut microbiome and resulted in a distinct microbiome structure.
28528593	3	49	theme	body	561:564	arg1	gain					573:576	the diet-induced body weight gain	544:576	the diet-induced body weight gain	544:576	A 30-d PM treatment significantly reduced the diet-induced body weight gain and blood TAG levels (P2·0).
28528593	1	50	theme	alginates	200:208	arg1	one					184:186	one	184:186	one	184:186	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	1	50	theme	alginates	200:208	arg1	alginates					200:208	numerous alginates	191:208	numerous alginates isolated from brown seaweeds	191:237	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	1	50	theme	alginates	200:208	arg1	acid					173:176	Polymannuronic acid	158:176	Polymannuronic acid (PM)	158:181	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
28528593	1	51	theme	brown	224:228	arg1	seaweeds					230:237	brown seaweeds	224:237	brown seaweeds	224:237	Polymannuronic acid (PM), one of numerous alginates isolated from brown seaweeds, is known to possess antioxidant activities.
26868870	4	0	theme	metabolic	734:742	arg1	signatures					744:753	maternal metabolic signatures	725:753	maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism	725:920	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	6	1	theme	prebiotic	1057:1065	arg1	intake					1067:1072	prebiotic intake	1057:1072	prebiotic intake during pregnancy and lactation	1057:1103	In summary, prebiotic intake during pregnancy and lactation improves maternal metabolism in diet-induced obese rats in a manner that attenuates the detrimental nutritional programming of offspring associated with maternal obesity.
26868870	5	2	theme	resistance	975:984	arg1	signature					954:962	the metabolomic signature	938:962	the metabolomic signature of insulin resistance in obese rats	938:998	In particular, the metabolomic signature of insulin resistance in obese rats normalized when dams consumed the prebiotic.
26868870	0	3	from	changes	13:19	arg1	microbiota					37:46	maternal gut microbiota	24:46	maternal gut microbiota	24:46	Diet-induced changes in maternal gut microbiota and metabolomic profiles influence programming of offspring obesity risk in rats.
26868870	0	3	from	changes	13:19	arg1	profiles					64:71	metabolomic profiles	52:71	metabolomic profiles	52:71	Diet-induced changes in maternal gut microbiota and metabolomic profiles influence programming of offspring obesity risk in rats.
26868870	5	4	from	signature	954:962	arg1	rats					995:998	obese rats	989:998	obese rats	989:998	In particular, the metabolomic signature of insulin resistance in obese rats normalized when dams consumed the prebiotic.
26868870	3	5	theme	gestational	533:543	arg1	gain					552:555	gestational weight gain	533:555	gestational weight gain	533:555	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	0	6	theme	obesity	108:114	arg1	risk					116:119	obesity risk	108:119	obesity risk	108:119	Diet-induced changes in maternal gut microbiota and metabolomic profiles influence programming of offspring obesity risk in rats.
26868870	7	7	theme	pre-clinical	1445:1456	arg1	evidence					1458:1465	compelling pre-clinical evidence	1434:1465	compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy	1434:1562	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
26868870	5	8	theme	metabolomic	942:952	arg1	signature					954:962	the metabolomic signature	938:962	the metabolomic signature of insulin resistance in obese rats	938:998	In particular, the metabolomic signature of insulin resistance in obese rats normalized when dams consumed the prebiotic.
26868870	4	9	theme	gut	689:691	arg1	microbiota					693:702	gut microbiota	689:702	gut microbiota	689:702	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	4	10	theme	Maternal	620:627	arg1	analysis					648:655	Maternal serum metabolomics analysis	620:655	Maternal serum metabolomics analysis	620:655	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	4	11	theme	obesity	805:811	arg1	risk					813:816	obesity risk	805:816	obesity risk	805:816	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	3	12	theme	increased	571:579	arg1	adiposity					581:589	increased adiposity	571:589	increased adiposity in dams and their offspring	571:617	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	5	13	theme	obese	989:993	arg1	rats					995:998	obese rats	989:998	obese rats	989:998	In particular, the metabolomic signature of insulin resistance in obese rats normalized when dams consumed the prebiotic.
26868870	6	14	theme	nutritional	1205:1215	arg1	programming					1217:1227	the detrimental nutritional programming	1189:1227	the detrimental nutritional programming of offspring associated with maternal obesity	1189:1273	In summary, prebiotic intake during pregnancy and lactation improves maternal metabolism in diet-induced obese rats in a manner that attenuates the detrimental nutritional programming of offspring associated with maternal obesity.
26868870	6	15	theme	maternal	1114:1121	arg1	metabolism					1123:1132	maternal metabolism	1114:1132	maternal metabolism in diet-induced obese rats	1114:1159	In summary, prebiotic intake during pregnancy and lactation improves maternal metabolism in diet-induced obese rats in a manner that attenuates the detrimental nutritional programming of offspring associated with maternal obesity.
26868870	2	16	theme	maternal	278:285	arg1	metabolism					287:296	improving maternal metabolism	268:296	improving maternal metabolism	268:296	In this context, improving maternal metabolism may help reduce the intergenerational transmission of obesity.
26868870	6	17	theme	diet-induced	1137:1148	arg1	rats					1156:1159	diet-induced obese rats	1137:1159	diet-induced obese rats	1137:1159	In summary, prebiotic intake during pregnancy and lactation improves maternal metabolism in diet-induced obese rats in a manner that attenuates the detrimental nutritional programming of offspring associated with maternal obesity.
26868870	3	18	theme	microbial	444:452	arg1	composition					454:464	obese maternal gut microbial composition	425:464	obese maternal gut microbial composition	425:464	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	4	19	theme	maternal	888:895	arg1	metabolism					911:920	maternal and offspring metabolism	888:920	maternal and offspring metabolism	888:920	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	2	20	theme	improving	268:276	arg1	metabolism					287:296	improving maternal metabolism	268:296	improving maternal metabolism	268:296	In this context, improving maternal metabolism may help reduce the intergenerational transmission of obesity.
26868870	6	21	theme	obese	1150:1154	arg1	rats					1156:1159	diet-induced obese rats	1137:1159	diet-induced obese rats	1137:1159	In summary, prebiotic intake during pregnancy and lactation improves maternal metabolism in diet-induced obese rats in a manner that attenuates the detrimental nutritional programming of offspring associated with maternal obesity.
26868870	3	22	theme	Sprague-Dawley	383:396	arg1	rats					398:401	Sprague-Dawley rats	383:401	Sprague-Dawley rats	383:401	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	7	23	theme	mechanisms	1348:1357	arg1	understanding					1318:1330	our understanding	1314:1330	our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity	1314:1420	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
26868870	2	24	theme	intergenerational	318:334	arg1	transmission					336:347	the intergenerational transmission	314:347	the intergenerational transmission of obesity	314:358	In this context, improving maternal metabolism may help reduce the intergenerational transmission of obesity.
26868870	3	25	theme	maternal	431:438	arg1	composition					454:464	obese maternal gut microbial composition	425:464	obese maternal gut microbial composition	425:464	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	0	26	theme	Diet-induced	0:11	arg1	changes					13:19	Diet-induced changes	0:19	Diet-induced changes in maternal gut microbiota and metabolomic profiles	0:71	Diet-induced changes in maternal gut microbiota and metabolomic profiles influence programming of offspring obesity risk in rats.
26868870	4	27	theme	offspring	901:909	arg1	metabolism					911:920	maternal and offspring metabolism	888:920	maternal and offspring metabolism	888:920	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	6	28	from	metabolism	1123:1132	arg1	rats					1156:1159	diet-induced obese rats	1137:1159	diet-induced obese rats	1137:1159	In summary, prebiotic intake during pregnancy and lactation improves maternal metabolism in diet-induced obese rats in a manner that attenuates the detrimental nutritional programming of offspring associated with maternal obesity.
26868870	6	29	theme	offspring	1232:1240	arg1	programming					1217:1227	the detrimental nutritional programming	1189:1227	the detrimental nutritional programming of offspring associated with maternal obesity	1189:1273	In summary, prebiotic intake during pregnancy and lactation improves maternal metabolism in diet-induced obese rats in a manner that attenuates the detrimental nutritional programming of offspring associated with maternal obesity.
26868870	3	30	theme	gut	440:442	arg1	composition					454:464	obese maternal gut microbial composition	425:464	obese maternal gut microbial composition	425:464	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	0	31	theme	maternal	24:31	arg1	microbiota					37:46	maternal gut microbiota	24:46	maternal gut microbiota	24:46	Diet-induced changes in maternal gut microbiota and metabolomic profiles influence programming of offspring obesity risk in rats.
26868870	4	32	dep	along	658:662	arg1	with					664:667	with	664:667	with	664:667	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	7	33	theme	offspring	1516:1524	arg1	outcomes					1536:1543	maternal and offspring metabolic outcomes	1503:1543	maternal and offspring metabolic outcomes	1503:1543	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
26868870	7	34	theme	maternal	1503:1510	arg1	outcomes					1536:1543	maternal and offspring metabolic outcomes	1503:1543	maternal and offspring metabolic outcomes	1503:1543	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
26868870	7	35	theme	obesity	1414:1420	arg1	programming					1389:1399	the developmental programming	1371:1399	the developmental programming of offspring obesity	1371:1420	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
26868870	6	36	theme	maternal	1258:1265	arg1	obesity					1267:1273	maternal obesity	1258:1273	maternal obesity	1258:1273	In summary, prebiotic intake during pregnancy and lactation improves maternal metabolism in diet-induced obese rats in a manner that attenuates the detrimental nutritional programming of offspring associated with maternal obesity.
26868870	4	37	theme	maternal	725:732	arg1	signatures					744:753	maternal metabolic signatures	725:753	maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism	725:920	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	1	38	theme	increased	211:219	arg1	risk					221:224	an increased risk	208:224	an increased risk of obesity in offspring	208:248	Maternal obesity and overnutrition during pregnancy and lactation can program an increased risk of obesity in offspring.
26868870	7	39	theme	offspring	1404:1412	arg1	obesity					1414:1420	offspring obesity	1404:1420	offspring obesity	1404:1420	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
26868870	4	40	dep	programming	783:793	arg1	offspring					795:803	offspring	795:803	offspring obesity risk	795:816	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	4	40	dep	programming	783:793	arg1	highlighted					822:832	highlighted	822:832	highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism	822:920	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	6	41	theme	detrimental	1193:1203	arg1	programming					1217:1227	the detrimental nutritional programming	1189:1227	the detrimental nutritional programming of offspring associated with maternal obesity	1189:1273	In summary, prebiotic intake during pregnancy and lactation improves maternal metabolism in diet-induced obese rats in a manner that attenuates the detrimental nutritional programming of offspring associated with maternal obesity.
26868870	4	42	theme	gut	870:872	arg1	microbiota					874:883	maternal gut microbiota	861:883	maternal gut microbiota	861:883	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	4	43	theme	microbiota	693:702	arg1	analysis					704:711	satiety hormone and gut microbiota analysis	669:711	satiety hormone and gut microbiota analysis	669:711	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	4	44	theme	hormone	677:683	arg1	analysis					704:711	satiety hormone and gut microbiota analysis	669:711	satiety hormone and gut microbiota analysis	669:711	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	0	45	theme	gut	33:35	arg1	microbiota					37:46	maternal gut microbiota	24:46	maternal gut microbiota	24:46	Diet-induced changes in maternal gut microbiota and metabolomic profiles influence programming of offspring obesity risk in rats.
26868870	7	46	theme	maternal	1339:1346	arg1	mechanisms					1348:1357	the maternal mechanisms	1335:1357	the maternal mechanisms influencing the developmental programming of offspring obesity	1335:1420	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
26868870	3	47	theme	obese	425:429	arg1	composition					454:464	obese maternal gut microbial composition	425:464	obese maternal gut microbial composition	425:464	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	4	48	theme	maternal	861:868	arg1	microbiota					874:883	maternal gut microbiota	861:883	maternal gut microbiota	861:883	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	1	49	from	risk	221:224	arg1	offspring					240:248	offspring	240:248	offspring	240:248	Maternal obesity and overnutrition during pregnancy and lactation can program an increased risk of obesity in offspring.
26868870	3	50	theme	prebiotic	471:479	arg1	treatment					481:489	prebiotic treatment	471:489	prebiotic treatment	471:489	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	0	51	theme	metabolomic	52:62	arg1	profiles					64:71	metabolomic profiles	52:71	metabolomic profiles	52:71	Diet-induced changes in maternal gut microbiota and metabolomic profiles influence programming of offspring obesity risk in rats.
26868870	5	52	theme	insulin	967:973	arg1	resistance					975:984	insulin resistance	967:984	insulin resistance in obese rats	967:998	In particular, the metabolomic signature of insulin resistance in obese rats normalized when dams consumed the prebiotic.
26868870	4	53	theme	serum	629:633	arg1	analysis					648:655	Maternal serum metabolomics analysis	620:655	Maternal serum metabolomics analysis	620:655	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	7	54	theme	compelling	1434:1443	arg1	evidence					1458:1465	compelling pre-clinical evidence	1434:1465	compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy	1434:1562	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
26868870	4	55	theme	microbiota	874:883	arg1	influence					848:856	the potential influence	834:856	the potential influence of maternal gut microbiota on maternal and offspring metabolism	834:920	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	7	56	theme	developmental	1375:1387	arg1	programming					1389:1399	the developmental programming	1371:1399	the developmental programming of offspring obesity	1371:1420	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
26868870	2	57	theme	obesity	352:358	arg1	transmission					336:347	the intergenerational transmission	314:347	the intergenerational transmission of obesity	314:358	In this context, improving maternal metabolism may help reduce the intergenerational transmission of obesity.
26868870	3	58	from	adiposity	581:589	arg1	dams					594:597	dams	594:597	dams	594:597	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	3	58	from	adiposity	581:589	arg1	offspring					609:617	their offspring	603:617	their offspring	603:617	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	5	59	from	resistance	975:984	arg1	rats					995:998	obese rats	989:998	obese rats	989:998	In particular, the metabolomic signature of insulin resistance in obese rats normalized when dams consumed the prebiotic.
26868870	7	60	theme	potential	1473:1481	arg1	strategy					1483:1490	a potential strategy	1471:1490	a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy	1471:1562	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
26868870	4	61	theme	potential	838:846	arg1	influence					848:856	the potential influence	834:856	the potential influence of maternal gut microbiota on maternal and offspring metabolism	834:920	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	7	62	theme	human	1548:1552	arg1	pregnancy					1554:1562	human pregnancy	1548:1562	human pregnancy	1548:1562	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
26868870	1	63	theme	Maternal	130:137	arg1	obesity					139:145	Maternal obesity	130:145	Maternal obesity	130:145	Maternal obesity and overnutrition during pregnancy and lactation can program an increased risk of obesity in offspring.
26868870	1	64	theme	obesity	229:235	arg1	risk					221:224	an increased risk	208:224	an increased risk of obesity in offspring	208:248	Maternal obesity and overnutrition during pregnancy and lactation can program an increased risk of obesity in offspring.
26868870	3	65	theme	energy	508:513	arg1	intake					515:520	maternal energy intake	499:520	maternal energy intake	499:520	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	5	66	from	rats	995:998	arg1	signature					954:962	the metabolomic signature	938:962	the metabolomic signature of insulin resistance in obese rats	938:998	In particular, the metabolomic signature of insulin resistance in obese rats normalized when dams consumed the prebiotic.
26868870	3	67	theme	maternal	499:506	arg1	intake					515:520	maternal energy intake	499:520	maternal energy intake	499:520	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	4	68	theme	satiety	669:675	arg1	hormone					677:683	satiety hormone	669:683	satiety hormone	669:683	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	4	69	from	influence	848:856	arg1	metabolism					911:920	maternal and offspring metabolism	888:920	maternal and offspring metabolism	888:920	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	3	70	theme	weight	545:550	arg1	gain					552:555	gestational weight gain	533:555	gestational weight gain	533:555	Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut microbial composition with prebiotic treatment reduces maternal energy intake, decreases gestational weight gain, and prevents increased adiposity in dams and their offspring.
26868870	4	71	theme	metabolomics	635:646	arg1	analysis					648:655	Maternal serum metabolomics analysis	620:655	Maternal serum metabolomics analysis	620:655	Maternal serum metabolomics analysis, along with satiety hormone and gut microbiota analysis, identified maternal metabolic signatures that could be implicated in programming offspring obesity risk and highlighted the potential influence of maternal gut microbiota on maternal and offspring metabolism.
26868870	7	72	theme	metabolic	1526:1534	arg1	outcomes					1536:1543	maternal and offspring metabolic outcomes	1503:1543	maternal and offspring metabolic outcomes	1503:1543	Overall, these findings contribute to our understanding of the maternal mechanisms influencing the developmental programming of offspring obesity and provide compelling pre-clinical evidence for a potential strategy to improve maternal and offspring metabolic outcomes in human pregnancy.
24211657	8	0	theme	other	1126:1130	arg1	formulations					1132:1143	the other formulations	1122:1143	the other formulations tested	1122:1150	The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation to the other formulations tested.
24211657	1	1	theme	drug	233:236	arg1	purposes					247:254	drug delivery purposes	233:254	drug delivery purposes	233:254	The use of drug-loaded nanoparticles and microparticles has been increasing, especially for cosmetic and drug delivery purposes.
24211657	7	2	theme	microparticles	951:964	arg1	biodistribution					928:942	the biodistribution	924:942	the biodistribution of the microparticles	924:964	The nuclear method used shows that the biodistribution of the microparticles remained in the skin.
24211657	3	3	theme	atomic	472:477	arg1	microscopy					485:494	atomic force microscopy	472:494	atomic force microscopy	472:494	In order to observe the morphological properties, Raman and atomic force microscopy evaluation were performed.
24211657	1	4	theme	delivery	238:245	arg1	purposes					247:254	drug delivery purposes	233:254	drug delivery purposes	233:254	The use of drug-loaded nanoparticles and microparticles has been increasing, especially for cosmetic and drug delivery purposes.
24211657	0	5	theme	in	86:87	arg1	analysis					99:106	an in vivo test analysis	83:106	an in vivo test analysis	83:106	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	0	5	theme	in	86:87	arg1	Microparticles					0:13	Microparticles	0:13	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.	0:126	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	4	6	theme	in	649:650	arg1	procedure					666:674	Technetium-99m and in vivo test was procedure	630:674	Technetium-99m and in vivo test was procedure to analyzed the cicatrization process	630:712	The biodistribution studies were analyzed by using a nuclear methodology, labeling the microparticles with Technetium-99m and in vivo test was procedure to analyzed the cicatrization process.
24211657	2	7	from	use	321:323	arg1	process					340:346	the healing process	328:346	the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan	328:409	In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan.
24211657	0	8	theme	test	94:97	arg1	analysis					99:106	an in vivo test analysis	83:106	an in vivo test analysis	83:106	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	0	8	theme	test	94:97	arg1	Microparticles					0:13	Microparticles	0:13	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.	0:126	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	0	9	dep	in	86:87	arg1	vivo					89:92	vivo	89:92	vivo	89:92	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	4	10	theme	test	657:660	arg1	procedure					666:674	Technetium-99m and in vivo test was procedure	630:674	Technetium-99m and in vivo test was procedure to analyzed the cicatrization process	630:712	The biodistribution studies were analyzed by using a nuclear methodology, labeling the microparticles with Technetium-99m and in vivo test was procedure to analyzed the cicatrization process.
24211657	1	11	theme	drug-loaded	139:149	arg1	nanoparticles					151:163	drug-loaded nanoparticles	139:163	drug-loaded nanoparticles	139:163	The use of drug-loaded nanoparticles and microparticles has been increasing, especially for cosmetic and drug delivery purposes.
24211657	5	12	theme	AFM	730:732	arg1	analysis					734:741	AFM analysis	730:741	AFM analysis	730:741	The results of AFM analysis show the formation and the adherence property of the microparticles.
24211657	8	13	theme	poloxamer	1035:1043	arg1	gel					1045:1047	the poloxamer gel	1031:1047	the poloxamer gel containing the microparticles	1031:1077	The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation to the other formulations tested.
24211657	8	14	theme	in	992:993	arg1	test					1014:1017	The in vivo cicatrization test	988:1017	The in vivo cicatrization test	988:1017	The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation to the other formulations tested.
24211657	3	15	dep	Raman	462:466	arg1	evaluation					496:505	evaluation	496:505	evaluation	496:505	In order to observe the morphological properties, Raman and atomic force microscopy evaluation were performed.
24211657	8	16	dep	in	992:993	arg1	vivo					995:998	vivo	995:998	vivo	995:998	The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation to the other formulations tested.
24211657	2	17	theme	burns	356:360	arg1	process					340:346	the healing process	328:346	the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan	328:409	In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan.
24211657	5	18	theme	analysis	734:741	arg1	results					719:725	The results	715:725	The results of AFM analysis	715:741	The results of AFM analysis show the formation and the adherence property of the microparticles.
24211657	2	19	theme	skin	351:354	arg1	burns					356:360	skin burns	351:360	skin burns in a composition of Aloe vera/vitamin E/chitosan	351:409	In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan.
24211657	6	20	from	distribution	836:847	arg1	microparticle					874:886	the microparticle	870:886	the microparticle	870:886	Raman analyses show the distribution of each component in the microparticle.
24211657	4	21	theme	biodistribution	527:541	arg1	studies					543:549	The biodistribution studies	523:549	The biodistribution studies	523:549	The biodistribution studies were analyzed by using a nuclear methodology, labeling the microparticles with Technetium-99m and in vivo test was procedure to analyzed the cicatrization process.
24211657	2	22	from	process	340:346	arg1	composition					367:377	a composition	365:377	a composition of Aloe vera/vitamin E/chitosan	365:409	In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan.
24211657	2	23	theme	microparticle	277:289	arg1	formulation					291:301	a new microparticle formulation	271:301	a new microparticle formulation	271:301	In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan.
24211657	1	24	theme	microparticles	169:182	arg1	use					132:134	The use	128:134	The use of drug-loaded nanoparticles and microparticles	128:182	The use of drug-loaded nanoparticles and microparticles has been increasing, especially for cosmetic and drug delivery purposes.
24211657	2	25	theme	E/chitosan	400:409	arg1	composition					367:377	a composition	365:377	a composition of Aloe vera/vitamin E/chitosan	365:409	In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan.
24211657	8	26	theme	cicatrization	1000:1012	arg1	test					1014:1017	The in vivo cicatrization test	988:1017	The in vivo cicatrization test	988:1017	The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation to the other formulations tested.
24211657	2	27	theme	new	273:275	arg1	formulation					291:301	a new microparticle formulation	271:301	a new microparticle formulation	271:301	In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan.
24211657	5	28	dep	formation	752:760	arg1	the					748:750	the	748:750	the	748:750	The results of AFM analysis show the formation and the adherence property of the microparticles.
24211657	6	29	theme	component	857:865	arg1	distribution					836:847	the distribution	832:847	the distribution of each component in the microparticle	832:886	Raman analyses show the distribution of each component in the microparticle.
24211657	8	30	theme	better	1086:1091	arg1	cicatrization					1093:1105	a better cicatrization	1084:1105	a better cicatrization	1084:1105	The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation to the other formulations tested.
24211657	7	31	theme	nuclear	893:899	arg1	method					901:906	The nuclear method	889:906	The nuclear method used	889:911	The nuclear method used shows that the biodistribution of the microparticles remained in the skin.
24211657	4	32	theme	Technetium-99m	630:643	arg1	procedure					666:674	Technetium-99m and in vivo test was procedure	630:674	Technetium-99m and in vivo test was procedure to analyzed the cicatrization process	630:712	The biodistribution studies were analyzed by using a nuclear methodology, labeling the microparticles with Technetium-99m and in vivo test was procedure to analyzed the cicatrization process.
24211657	0	33	theme	E/chitosan	36:45	arg1	imaging					71:77	a nuclear imaging	61:77	a nuclear imaging	61:77	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	0	33	theme	E/chitosan	36:45	arg1	Microparticles					0:13	Microparticles	0:13	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.	0:126	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	0	33	theme	E/chitosan	36:45	arg1	analysis					99:106	an in vivo test analysis	83:106	an in vivo test analysis	83:106	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	0	34	theme	burn	112:115	arg1	treatment					117:125	burn treatment	112:125	burn treatment	112:125	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	5	35	theme	microparticles	796:809	arg1	property					780:787	the adherence property	766:787	the adherence property	766:787	The results of AFM analysis show the formation and the adherence property of the microparticles.
24211657	5	35	theme	microparticles	796:809	arg1	formation					752:760	formation	752:760	formation	752:760	The results of AFM analysis show the formation and the adherence property of the microparticles.
24211657	5	36	theme	adherence	770:778	arg1	property					780:787	the adherence property	766:787	the adherence property	766:787	The results of AFM analysis show the formation and the adherence property of the microparticles.
24211657	8	37	contain	containing	1049:1058	arg1	gel					1045:1047	the poloxamer gel	1031:1047	the poloxamer gel containing the microparticles	1031:1077	The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation to the other formulations tested.
24211657	8	37	contain	containing	1049:1058	arg2	microparticles					1064:1077	the microparticles	1060:1077	the microparticles	1060:1077	The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation to the other formulations tested.
24211657	4	38	theme	cicatrization	692:704	arg1	process					706:712	the cicatrization process	688:712	the cicatrization process	688:712	The biodistribution studies were analyzed by using a nuclear methodology, labeling the microparticles with Technetium-99m and in vivo test was procedure to analyzed the cicatrization process.
24211657	0	39	theme	microscopic	48:58	arg1	imaging					71:77	a nuclear imaging	61:77	a nuclear imaging	61:77	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	0	39	theme	microscopic	48:58	arg1	Microparticles					0:13	Microparticles	0:13	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.	0:126	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	2	40	from	burns	356:360	arg1	composition					367:377	a composition	365:377	a composition of Aloe vera/vitamin E/chitosan	365:409	In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan.
24211657	3	41	theme	morphological	436:448	arg1	properties					450:459	the morphological properties	432:459	the morphological properties	432:459	In order to observe the morphological properties, Raman and atomic force microscopy evaluation were performed.
24211657	2	42	from	composition	367:377	arg1	process					340:346	the healing process	328:346	the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan	328:409	In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan.
24211657	4	43	theme	was	662:664	arg1	procedure					666:674	Technetium-99m and in vivo test was procedure	630:674	Technetium-99m and in vivo test was procedure to analyzed the cicatrization process	630:712	The biodistribution studies were analyzed by using a nuclear methodology, labeling the microparticles with Technetium-99m and in vivo test was procedure to analyzed the cicatrization process.
24211657	4	44	theme	nuclear	576:582	arg1	methodology					584:594	a nuclear methodology	574:594	a nuclear methodology	574:594	The biodistribution studies were analyzed by using a nuclear methodology, labeling the microparticles with Technetium-99m and in vivo test was procedure to analyzed the cicatrization process.
24211657	0	45	theme	nuclear	63:69	arg1	imaging					71:77	a nuclear imaging	61:77	a nuclear imaging	61:77	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	0	45	theme	nuclear	63:69	arg1	Microparticles					0:13	Microparticles	0:13	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.	0:126	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	6	46	theme	Raman	812:816	arg1	analyses					818:825	Raman analyses	812:825	Raman analyses	812:825	Raman analyses show the distribution of each component in the microparticle.
24211657	4	47	dep	in	649:650	arg1	vivo					652:655	vivo	652:655	vivo	652:655	The biodistribution studies were analyzed by using a nuclear methodology, labeling the microparticles with Technetium-99m and in vivo test was procedure to analyzed the cicatrization process.
24211657	3	48	theme	force	479:483	arg1	microscopy					485:494	atomic force microscopy	472:494	atomic force microscopy	472:494	In order to observe the morphological properties, Raman and atomic force microscopy evaluation were performed.
24211657	1	49	theme	nanoparticles	151:163	arg1	use					132:134	The use	128:134	The use of drug-loaded nanoparticles and microparticles	128:182	The use of drug-loaded nanoparticles and microparticles has been increasing, especially for cosmetic and drug delivery purposes.
24211657	0	50	dep	Microparticles	0:13	arg1	imaging					71:77	a nuclear imaging	61:77	a nuclear imaging	61:77	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	0	50	dep	Microparticles	0:13	arg1	analysis					99:106	an in vivo test analysis	83:106	an in vivo test analysis	83:106	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	0	50	dep	Microparticles	0:13	arg1	Microparticles					0:13	Microparticles	0:13	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.	0:126	Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
24211657	2	51	theme	healing	332:338	arg1	process					340:346	the healing process	328:346	the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan	328:409	In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan.
27401929	7	0	theme	bacteria	1903:1910	arg1	diversity					1884:1892	greater diversity	1876:1892	greater diversity of those bacteria considered beneficial to gut health	1876:1946	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	5	1	located	observed	1215:1222	arg1	Cae					1284:1286	Cae	1284:1286	Cae	1284:1286	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	5	1	located	observed	1215:1222	arg1	DC					1292:1293	DC	1292:1293	DC	1292:1293	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	5	1	located	observed	1215:1222	arg2	changes					1202:1208	notable changes	1194:1208	notable changes	1194:1208	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	3	2	theme	wheat	602:606	arg1	WS					616:617	WS	616:617	WS	616:617	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	3	2	theme	wheat	602:606	arg1	starch					608:613	wheat starch	602:613	wheat starch (WS) only	602:623	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	2	3	theme	communities	525:535	arg1	modification					486:497	a modification	484:497	a modification of the resident bacterial communities	484:535	We hypothesised that feeding pigs these SDF would stimulate Cae and DC carbohydrate fermentation, resulting in a modification of the resident bacterial communities.
27401929	4	4	theme	end-products	869:880	arg1	analysis					844:851	analysis	844:851	analysis of fermentative end-products	844:880	Post-euthanasia, Cae and DC digesta were collected for analysis of fermentative end-products, and bacterial community profiles were determined by 16S rRNA gene amplicon 454 pyrosequencing.
27401929	6	5	theme	bacterial	1364:1372	arg1	profiles					1374:1381	influencing bacterial profiles	1352:1381	influencing bacterial profiles	1352:1381	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	1	6	theme	linkage	237:243	arg1	MLG					254:256	MLG	254:256	MLG	254:256	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	6	theme	linkage	237:243	arg1	glucans					245:251	oat-mixed linkage glucans	227:251	oat-mixed linkage glucans (MLG)	227:257	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	6	theme	linkage	237:243	arg1	fibres					185:190	two cereal soluble dietary fibres	158:190	two cereal soluble dietary fibres (SDF)	158:196	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	7	7	theme	greater	1876:1882	arg1	diversity					1884:1892	greater diversity	1876:1892	greater diversity of those bacteria considered beneficial to gut health	1876:1946	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	7	8	theme	AX	1630:1631	arg1	SDF					1626:1628	the cereal SDF AX	1615:1631	the cereal SDF AX	1615:1631	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	4	9	theme	rRNA	939:942	arg1	pyrosequencing					962:975	16S rRNA gene amplicon 454 pyrosequencing	935:975	16S rRNA gene amplicon 454 pyrosequencing	935:975	Post-euthanasia, Cae and DC digesta were collected for analysis of fermentative end-products, and bacterial community profiles were determined by 16S rRNA gene amplicon 454 pyrosequencing.
27401929	1	10	theme	soluble	169:175	arg1	SDF					193:195	SDF	193:195	SDF	193:195	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	10	theme	soluble	169:175	arg1	glucans					245:251	oat-mixed linkage glucans	227:251	oat-mixed linkage glucans (MLG)	227:257	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	10	theme	soluble	169:175	arg1	fibres					185:190	two cereal soluble dietary fibres	158:190	two cereal soluble dietary fibres (SDF)	158:196	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	10	theme	soluble	169:175	arg1	arabinoxylan					205:216	wheat arabinoxylan	199:216	wheat arabinoxylan (AX)	199:221	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	7	11	theme	cereal	1619:1624	arg1	SDF					1626:1628	the cereal SDF AX	1615:1631	the cereal SDF AX	1615:1631	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	4	12	theme	amplicon	949:956	arg1	pyrosequencing					962:975	16S rRNA gene amplicon 454 pyrosequencing	935:975	16S rRNA gene amplicon 454 pyrosequencing	935:975	Post-euthanasia, Cae and DC digesta were collected for analysis of fermentative end-products, and bacterial community profiles were determined by 16S rRNA gene amplicon 454 pyrosequencing.
27401929	6	13	theme	greatest	1333:1340	arg1	effect					1342:1347	the greatest effect	1329:1347	the greatest effect of influencing bacterial profiles	1329:1381	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	1	14	theme	fibres	185:190	arg1	effects					147:153	the effects	143:153	the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC)	143:370	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	7	15	from	rich	1767:1770	arg1	carbohydrates					1792:1804	specific complex carbohydrates	1775:1804	specific complex carbohydrates	1775:1804	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	3	16	theme	treatment	629:637	arg1	diets					639:643	treatment diets	629:643	treatment diets	629:643	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	6	17	theme	fatty	1528:1532	arg1	acid					1534:1537	short-chain fatty acid	1516:1537	short-chain fatty acid	1516:1537	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	7	18	theme	bacterial	1816:1824	arg1	population					1826:1835	the bacterial population	1812:1835	the bacterial population	1812:1835	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	7	19	from	carbohydrates	1792:1804	arg1	rich					1767:1770	rich	1767:1770	rich	1767:1770	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	2	20	theme	resident	506:513	arg1	communities					525:535	the resident bacterial communities	502:535	the resident bacterial communities	502:535	We hypothesised that feeding pigs these SDF would stimulate Cae and DC carbohydrate fermentation, resulting in a modification of the resident bacterial communities.
27401929	1	21	dep	arabinoxylan	205:216	arg1	AX					219:220	AX	219:220	AX	219:220	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	22	theme	distal	354:359	arg1	colon					361:365	distal colon	354:365	distal colon (DC)	354:370	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	22	theme	distal	354:359	arg1	DC					368:369	DC	368:369	DC	368:369	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	6	23	theme	associated	1492:1501	arg1	increases					1503:1511	associated increases	1492:1511	associated increases in short-chain fatty acid and reduced ammonia concentrations	1492:1572	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	6	24	theme	sequence	1393:1400	arg1	proportions					1402:1412	sequence proportions	1393:1412	sequence proportions assigned to the genus Clostridium, considered detrimental to gut health,	1393:1485	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	0	25	theme	porcine	54:60	arg1	diets					62:66	porcine diets	54:66	porcine diets	54:66	Addition of arabinoxylan and mixed linkage glucans in porcine diets affects the large intestinal bacterial populations.
27401929	3	26	theme	%	719:719	arg1	AXMLG					733:737	AXMLG	733:737	AXMLG	733:737	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	3	26	theme	%	719:719	arg1	MLG					728:730	5 % AX:5 % MLG	717:730	5 % AX:5 % MLG (AXMLG)	717:738	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	7	27	theme	gut	1937:1939	arg1	health					1941:1946	gut health	1937:1946	gut health	1937:1946	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	5	28	theme	intestine	1073:1081	arg1	region					1053:1058	the proximal region	1040:1058	the proximal region of the large intestine	1040:1081	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	3	29	theme	10 	690:692	arg1	%					693:693	%	693:693	%	693:693	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	1	30	theme	community	303:311	arg1	profiles					313:320	bacterial community profiles	293:320	bacterial community profiles	293:320	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	0	31	from	Addition	0:7	arg1	diets					62:66	porcine diets	54:66	porcine diets	54:66	Addition of arabinoxylan and mixed linkage glucans in porcine diets affects the large intestinal bacterial populations.
27401929	7	32	dep	CONCLUSIONS	1575:1585	arg1	demonstrated					1598:1609	demonstrated	1598:1609	demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health	1598:1946	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	0	33	theme	large	80:84	arg1	populations					107:117	the large intestinal bacterial populations	76:117	the large intestinal bacterial populations	76:117	Addition of arabinoxylan and mixed linkage glucans in porcine diets affects the large intestinal bacterial populations.
27401929	2	34	theme	DC	441:442	arg1	fermentation					457:468	DC carbohydrate fermentation	441:468	DC carbohydrate fermentation	441:468	We hypothesised that feeding pigs these SDF would stimulate Cae and DC carbohydrate fermentation, resulting in a modification of the resident bacterial communities.
27401929	6	35	theme	ammonia	1551:1557	arg1	concentrations					1559:1572	reduced ammonia concentrations	1543:1572	reduced ammonia concentrations	1543:1572	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	0	36	theme	bacterial	97:105	arg1	populations					107:117	the large intestinal bacterial populations	76:117	the large intestinal bacterial populations	76:117	Addition of arabinoxylan and mixed linkage glucans in porcine diets affects the large intestinal bacterial populations.
27401929	1	37	theme	porcine	329:335	arg1	Cae					345:347	Cae	345:347	Cae	345:347	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	37	theme	porcine	329:335	arg1	caecum					337:342	the porcine caecum	325:342	the porcine caecum (Cae)	325:348	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	6	38	contain	had	1325:1327	arg2	effect					1342:1347	the greatest effect	1329:1347	the greatest effect of influencing bacterial profiles	1329:1381	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	6	38	contain	had	1325:1327	arg1	addition					1300:1307	The addition	1296:1307	The addition of MLG or AXMLG	1296:1323	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	3	39	theme	%	726:726	arg1	AXMLG					733:737	AXMLG	733:737	AXMLG	733:737	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	3	39	theme	%	726:726	arg1	MLG					728:730	5 % AX:5 % MLG	717:730	5 % AX:5 % MLG (AXMLG)	717:738	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	7	40	theme	rich	1767:1770	arg1	diets					1761:1765	diets	1761:1765	diets rich in specific complex carbohydrates	1761:1804	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	0	41	theme	arabinoxylan	12:23	arg1	Addition					0:7	Addition	0:7	Addition of arabinoxylan and mixed linkage glucans in porcine diets	0:66	Addition of arabinoxylan and mixed linkage glucans in porcine diets affects the large intestinal bacterial populations.
27401929	5	42	theme	SDF-containing	1001:1014	arg1	diets					1016:1020	all the SDF-containing diets	993:1020	all the SDF-containing diets	993:1020	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	0	43	theme	mixed	29:33	arg1	glucans					43:49	mixed linkage glucans	29:49	mixed linkage glucans	29:49	Addition of arabinoxylan and mixed linkage glucans in porcine diets affects the large intestinal bacterial populations.
27401929	3	44	theme	ground	769:774	arg1	wheat					782:786	ground whole wheat	769:786	ground whole wheat	769:786	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	6	45	from	increases	1503:1511	arg1	concentrations					1559:1572	reduced ammonia concentrations	1543:1572	reduced ammonia concentrations	1543:1572	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	6	45	from	increases	1503:1511	arg1	acid					1534:1537	short-chain fatty acid	1516:1537	short-chain fatty acid	1516:1537	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	0	46	theme	glucans	43:49	arg1	Addition					0:7	Addition	0:7	Addition of arabinoxylan and mixed linkage glucans in porcine diets	0:66	Addition of arabinoxylan and mixed linkage glucans in porcine diets affects the large intestinal bacterial populations.
27401929	1	47	theme	fermentative	263:274	arg1	end-products					276:287	fermentative end-products	263:287	fermentative end-products	263:287	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	7	48	theme	bacterial	1670:1678	arg1	composition					1690:1700	the large intestinal bacterial community composition	1649:1700	the large intestinal bacterial community composition	1649:1700	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	4	49	theme	bacterial	887:895	arg1	profiles					907:914	bacterial community profiles	887:914	bacterial community profiles	887:914	Post-euthanasia, Cae and DC digesta were collected for analysis of fermentative end-products, and bacterial community profiles were determined by 16S rRNA gene amplicon 454 pyrosequencing.
27401929	6	50	theme	MLG	1312:1314	arg1	addition					1300:1307	The addition	1296:1307	The addition of MLG or AXMLG	1296:1323	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	1	51	theme	wheat	199:203	arg1	fibres					185:190	two cereal soluble dietary fibres	158:190	two cereal soluble dietary fibres (SDF)	158:196	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	51	theme	wheat	199:203	arg1	arabinoxylan					205:216	wheat arabinoxylan	199:216	wheat arabinoxylan (AX)	199:221	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	7	52	theme	large	1653:1657	arg1	composition					1690:1700	the large intestinal bacterial community composition	1649:1700	the large intestinal bacterial community composition	1649:1700	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	4	53	theme	fermentative	856:867	arg1	end-products					869:880	fermentative end-products	856:880	fermentative end-products	856:880	Post-euthanasia, Cae and DC digesta were collected for analysis of fermentative end-products, and bacterial community profiles were determined by 16S rRNA gene amplicon 454 pyrosequencing.
27401929	5	54	from	RESULTS	978:984	arg1	region					1053:1058	the proximal region	1040:1058	the proximal region of the large intestine	1040:1081	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	5	55	theme	notable	1194:1200	arg1	changes					1202:1208	notable changes	1194:1208	notable changes	1194:1208	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	1	56	theme	oat-mixed	227:235	arg1	MLG					254:256	MLG	254:256	MLG	254:256	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	56	theme	oat-mixed	227:235	arg1	glucans					245:251	oat-mixed linkage glucans	227:251	oat-mixed linkage glucans (MLG)	227:257	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	56	theme	oat-mixed	227:235	arg1	fibres					185:190	two cereal soluble dietary fibres	158:190	two cereal soluble dietary fibres (SDF)	158:196	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	2	57	theme	bacterial	515:523	arg1	communities					525:535	the resident bacterial communities	502:535	the resident bacterial communities	502:535	We hypothesised that feeding pigs these SDF would stimulate Cae and DC carbohydrate fermentation, resulting in a modification of the resident bacterial communities.
27401929	4	58	theme	gene	944:947	arg1	pyrosequencing					962:975	16S rRNA gene amplicon 454 pyrosequencing	935:975	16S rRNA gene amplicon 454 pyrosequencing	935:975	Post-euthanasia, Cae and DC digesta were collected for analysis of fermentative end-products, and bacterial community profiles were determined by 16S rRNA gene amplicon 454 pyrosequencing.
27401929	6	59	theme	influencing	1352:1362	arg1	profiles					1374:1381	influencing bacterial profiles	1352:1381	influencing bacterial profiles	1352:1381	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	1	60	theme	cereal	162:167	arg1	SDF					193:195	SDF	193:195	SDF	193:195	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	60	theme	cereal	162:167	arg1	glucans					245:251	oat-mixed linkage glucans	227:251	oat-mixed linkage glucans (MLG)	227:257	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	60	theme	cereal	162:167	arg1	fibres					185:190	two cereal soluble dietary fibres	158:190	two cereal soluble dietary fibres (SDF)	158:196	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	60	theme	cereal	162:167	arg1	arabinoxylan					205:216	wheat arabinoxylan	199:216	wheat arabinoxylan (AX)	199:221	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	5	61	theme	Ruminococcaceae	1232:1246	arg1	families					1268:1275	the Ruminococcaceae and Lachnospiraceae families	1228:1275	families	1268:1275	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	1	62	theme	dietary	177:183	arg1	SDF					193:195	SDF	193:195	SDF	193:195	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	62	theme	dietary	177:183	arg1	glucans					245:251	oat-mixed linkage glucans	227:251	oat-mixed linkage glucans (MLG)	227:257	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	62	theme	dietary	177:183	arg1	fibres					185:190	two cereal soluble dietary fibres	158:190	two cereal soluble dietary fibres (SDF)	158:196	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	62	theme	dietary	177:183	arg1	arabinoxylan					205:216	wheat arabinoxylan	199:216	wheat arabinoxylan (AX)	199:221	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	5	63	theme	Lachnospiraceae	1252:1266	arg1	families					1268:1275	the Ruminococcaceae and Lachnospiraceae families	1228:1275	families	1268:1275	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	4	64	theme	16S	935:937	arg1	rRNA					939:942	16S rRNA	935:942	16S rRNA gene amplicon 454 pyrosequencing	935:975	Post-euthanasia, Cae and DC digesta were collected for analysis of fermentative end-products, and bacterial community profiles were determined by 16S rRNA gene amplicon 454 pyrosequencing.
27401929	6	65	theme	AXMLG	1319:1323	arg1	addition					1300:1307	The addition	1296:1307	The addition of MLG or AXMLG	1296:1323	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	6	66	theme	short-chain	1516:1526	arg1	acid					1534:1537	short-chain fatty acid	1516:1537	short-chain fatty acid	1516:1537	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	1	67	theme	colon	361:365	arg1	profiles					313:320	bacterial community profiles	293:320	bacterial community profiles	293:320	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	67	theme	colon	361:365	arg1	end-products					276:287	fermentative end-products	263:287	fermentative end-products	263:287	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	5	68	dep	Cae	1284:1286	arg1	the					1280:1282	the	1280:1282	the	1280:1282	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	7	69	theme	community	1680:1688	arg1	composition					1690:1700	the large intestinal bacterial community composition	1649:1700	the large intestinal bacterial community composition	1649:1700	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	3	70	theme	AX	683:684	arg1	%					681:681	10 % AX	678:684	10 % AX	678:684	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	5	71	theme	proximal	1044:1051	arg1	region					1053:1058	the proximal region	1040:1058	the proximal region of the large intestine	1040:1081	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	5	72	theme	large	1067:1071	arg1	intestine					1073:1081	the large intestine	1063:1081	the large intestine	1063:1081	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	1	73	theme	bacterial	293:301	arg1	profiles					313:320	bacterial community profiles	293:320	bacterial community profiles	293:320	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	6	74	theme	profiles	1374:1381	arg1	effect					1342:1347	the greatest effect	1329:1347	the greatest effect of influencing bacterial profiles	1329:1381	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	3	75	theme	AX:5 	721:725	arg1	AXMLG					733:737	AXMLG	733:737	AXMLG	733:737	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	3	75	theme	AX:5 	721:725	arg1	MLG					728:730	5 % AX:5 % MLG	717:730	5 % AX:5 % MLG (AXMLG)	717:738	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	3	76	dep	METHODS	538:544	arg1	replaced					755:762	replaced	755:762	replaced with ground whole wheat	755:786	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	3	76	dep	METHODS	538:544	arg1	fed					580:582	fed	580:582	fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG)	580:738	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	3	77	theme	%	693:693	arg1	MLG					695:697	10 % MLG	690:697	10 % MLG	690:697	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	3	77	theme	%	693:693	arg1	combination					702:712	a combination	700:712	a combination of 5 % AX:5 % MLG (AXMLG)	700:738	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	0	78	theme	intestinal	86:95	arg1	populations					107:117	the large intestinal bacterial populations	76:117	the large intestinal bacterial populations	76:117	Addition of arabinoxylan and mixed linkage glucans in porcine diets affects the large intestinal bacterial populations.
27401929	7	79	theme	complex	1784:1790	arg1	carbohydrates					1792:1804	specific complex carbohydrates	1775:1804	specific complex carbohydrates	1775:1804	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	6	80	theme	gut	1475:1477	arg1	health					1479:1484	gut health	1475:1484	gut health	1475:1484	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	3	81	theme	5 	717:718	arg1	%					719:719	%	719:719	%	719:719	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	2	82	theme	carbohydrate	444:455	arg1	fermentation					457:468	DC carbohydrate fermentation	441:468	DC carbohydrate fermentation	441:468	We hypothesised that feeding pigs these SDF would stimulate Cae and DC carbohydrate fermentation, resulting in a modification of the resident bacterial communities.
27401929	1	83	from	effects	147:153	arg1	profiles					313:320	bacterial community profiles	293:320	bacterial community profiles	293:320	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	83	from	effects	147:153	arg1	end-products					276:287	fermentative end-products	263:287	fermentative end-products	263:287	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	6	84	theme	reduced	1543:1549	arg1	concentrations					1559:1572	reduced ammonia concentrations	1543:1572	reduced ammonia concentrations	1543:1572	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	1	85	theme	caecum	337:342	arg1	profiles					313:320	bacterial community profiles	293:320	bacterial community profiles	293:320	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	1	85	theme	caecum	337:342	arg1	end-products					276:287	fermentative end-products	263:287	fermentative end-products	263:287	PURPOSE To investigate the effects of two cereal soluble dietary fibres (SDF), wheat arabinoxylan (AX) and oat-mixed linkage glucans (MLG), on fermentative end-products and bacterial community profiles of the porcine caecum (Cae) and distal colon (DC).
27401929	3	86	theme	MLG	728:730	arg1	MLG					695:697	10 % MLG	690:697	10 % MLG	690:697	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	3	86	theme	MLG	728:730	arg1	combination					702:712	a combination	700:712	a combination of 5 % AX:5 % MLG (AXMLG)	700:738	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	6	87	theme	genus	1430:1434	arg1	Clostridium					1436:1446	the genus Clostridium	1426:1446	the genus Clostridium	1426:1446	The addition of MLG or AXMLG had the greatest effect of influencing bacterial profiles, reducing sequence proportions assigned to the genus Clostridium, considered detrimental to gut health, with associated increases in short-chain fatty acid and reduced ammonia concentrations.
27401929	0	88	theme	linkage	35:41	arg1	glucans					43:49	mixed linkage glucans	29:49	mixed linkage glucans	29:49	Addition of arabinoxylan and mixed linkage glucans in porcine diets affects the large intestinal bacterial populations.
27401929	3	89	theme	pigs	565:568	arg1	groups					551:556	Five groups	546:556	Five groups of six pigs	546:568	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	3	89	theme	pigs	565:568	arg1	pigs					565:568	six pigs	561:568	six pigs	561:568	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	3	90	theme	whole	776:780	arg1	wheat					782:786	ground whole wheat	769:786	ground whole wheat	769:786	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27401929	4	91	theme	DC	814:815	arg1	digesta					817:823	DC digesta	814:823	DC digesta	814:823	Post-euthanasia, Cae and DC digesta were collected for analysis of fermentative end-products, and bacterial community profiles were determined by 16S rRNA gene amplicon 454 pyrosequencing.
27401929	7	92	theme	specific	1775:1782	arg1	carbohydrates					1792:1804	specific complex carbohydrates	1775:1804	specific complex carbohydrates	1775:1804	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	5	93	dep	influenced	1165:1174	arg1	P < 0.05					1177:1184	P < 0.05	1177:1184	P < 0.05	1177:1184	RESULTS Across all the SDF-containing diets, predominantly in the proximal region of the large intestine, Prevotella, Lactobacillus, Mitsuokella and Streptococcus were most significantly influenced (P < 0.05), while notable changes were observed for the Ruminococcaceae and Lachnospiraceae families in the Cae and DC.
27401929	7	94	theme	intestinal	1659:1668	arg1	composition					1690:1700	the large intestinal bacterial community composition	1649:1700	the large intestinal bacterial community composition	1649:1700	CONCLUSIONS This study demonstrated how the cereal SDF AX and MLG altered the large intestinal bacterial community composition, particularly proximally, further giving insights into how diets rich in specific complex carbohydrates shift the bacterial population, by increasing abundance and promoting greater diversity of those bacteria considered beneficial to gut health.
27401929	4	95	theme	community	897:905	arg1	profiles					907:914	bacterial community profiles	887:914	bacterial community profiles	887:914	Post-euthanasia, Cae and DC digesta were collected for analysis of fermentative end-products, and bacterial community profiles were determined by 16S rRNA gene amplicon 454 pyrosequencing.
27401929	3	96	dep	fed	580:582	arg1	each					575:578	each	575:578	each	575:578	METHODS Five groups of six pigs were each fed one diet based on wheat starch (WS) only, or treatment diets in which some WS was replaced by 10 % AX, or 10 % MLG, a combination of 5 % AX:5 % MLG (AXMLG), or completely replaced with ground whole wheat.
27229861	7	0	dep	100 	1608:1611	arg1	to					1605:1606	to	1605:1606	to	1605:1606	The tool was validated on 24 independent genomic datasets and showed up to 100 % sensitivity and 0.94 MCC.
27229861	4	1	theme	novel	1072:1076	arg1	hydrolases					1092:1101	novel peptidoglycan hydrolases	1072:1101	novel peptidoglycan hydrolases from genomic and metagenomic data	1072:1135	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	6	2	theme	Forest	1317:1322	arg1	model					1335:1339	Random Forest multiclass model	1310:1339	Random Forest multiclass model	1310:1339	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	3	3	theme	peptidoglycan	543:555	arg1	turnover					557:564	overall peptidoglycan turnover	535:564	overall peptidoglycan turnover	535:564	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	3	4	theme	tool	727:730	arg1	development					696:706	development	696:706	development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data	696:840	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	10	5	theme	genomic	2008:2014	arg1	data					2032:2035	genomic and metagenomic data	2008:2035	genomic and metagenomic data	2008:2035	To our knowledge, this is the only tool available for the prediction of peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	6	theme	peptidoglycan	876:888	arg1	RESULTS					843:849	RESULTS	843:849	RESULTS In this study	843:863	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	6	theme	peptidoglycan	876:888	arg1	hydrolases					890:899	the known peptidoglycan hydrolases	866:899	the known peptidoglycan hydrolases	866:899	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	1	7	theme	potential	251:259	arg1	alternatives					261:272	potential alternatives	251:272	potential alternatives to antibiotics	251:287	BACKGROUND The efficacy of antibiotics against bacterial infections is decreasing due to the development of resistance in bacteria, and thus, there is a need to search for potential alternatives to antibiotics.
27229861	9	8	from	ORFs	1900:1903	arg1	classification					1814:1827	classification	1814:1827	classification	1814:1827	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	9	8	from	ORFs	1900:1903	arg1	identification					1795:1808	identification	1795:1808	identification	1795:1808	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	9	8	from	ORFs	1900:1903	arg1	hydrolases					1852:1861	novel peptidoglycan hydrolases	1832:1861	novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs	1832:1903	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	10	9	theme	hydrolases	1992:2001	arg1	prediction					1964:1973	the prediction	1960:1973	the prediction of peptidoglycan hydrolases from genomic and metagenomic data	1960:2035	To our knowledge, this is the only tool available for the prediction of peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	10	theme	metagenomic	1120:1130	arg1	data					1132:1135	genomic and metagenomic data	1108:1135	genomic and metagenomic data	1108:1135	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	3	11	from	identification	740:753	arg1	data					837:840	genomic and metagenomic data	813:840	genomic and metagenomic data	813:840	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	6	12	theme	tool	1382:1385	arg1	development					1362:1372	the development	1358:1372	the development of HyPe tool	1358:1385	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	1	13	theme	bacterial	126:134	arg1	infections					136:145	bacterial infections	126:145	bacterial infections	126:145	BACKGROUND The efficacy of antibiotics against bacterial infections is decreasing due to the development of resistance in bacteria, and thus, there is a need to search for potential alternatives to antibiotics.
27229861	4	14	theme	action	959:964	arg1	site					951:954	their site	945:954	their site of action	945:964	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	14	theme	action	959:964	arg1	action					959:964	action	959:964	action	959:964	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	10	15	theme	metagenomic	2020:2030	arg1	data					2032:2035	genomic and metagenomic data	2008:2035	genomic and metagenomic data	2008:2035	To our knowledge, this is the only tool available for the prediction of peptidoglycan hydrolases from genomic and metagenomic data.
27229861	3	16	theme	cell	592:595	arg1	separation					597:606	daughter cell separation	583:606	daughter cell separation	583:606	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	5	17	theme	composition	1214:1224	arg1	features					1226:1233	amino acid and dipeptide composition features	1189:1233	amino acid and dipeptide composition features	1189:1233	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	3	18	theme	peptidoglycan	783:795	arg1	hydrolases					797:806	novel peptidoglycan hydrolases	777:806	novel peptidoglycan hydrolases from genomic and metagenomic data	777:840	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	19	used	used	975:978	arg2	RESULTS					843:849	RESULTS	843:849	RESULTS In this study	843:863	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	19	used	used	975:978	arg2	hydrolases					890:899	the known peptidoglycan hydrolases	866:899	the known peptidoglycan hydrolases	866:899	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	1	20	from	bacteria	201:208	arg1	development					172:182	the development	168:182	the development of resistance in bacteria	168:208	BACKGROUND The efficacy of antibiotics against bacterial infections is decreasing due to the development of resistance in bacteria, and thus, there is a need to search for potential alternatives to antibiotics.
27229861	6	21	theme	multiclass	1324:1333	arg1	model					1335:1339	Random Forest multiclass model	1310:1339	Random Forest multiclass model	1310:1339	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	11	22	theme	AVAILABILITY	2038:2049	arg1	http					2051:2054	AVAILABILITY http	2038:2054	AVAILABILITY http	2038:2054	AVAILABILITY http://metagenomics.iiserb.ac.in/hype/ and http://metabiosys.iiserb.ac.in/hype/ .
27229861	1	23	from	resistance	187:196	arg1	bacteria					201:208	bacteria	201:208	bacteria	201:208	BACKGROUND The efficacy of antibiotics against bacterial infections is decreasing due to the development of resistance in bacteria, and thus, there is a need to search for potential alternatives to antibiotics.
27229861	0	24	theme	antibacterial	55:67	arg1	proteins					69:76	antibacterial proteins	55:76	antibacterial proteins	55:76	Prediction of peptidoglycan hydrolases- a new class of antibacterial proteins.
27229861	5	25	theme	classification	1146:1159	arg1	models					1161:1166	Various classification models	1138:1166	Various classification models	1138:1166	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	0	26	theme	peptidoglycan	14:26	arg1	Prediction					0:9	Prediction	0:9	Prediction of peptidoglycan	0:26	Prediction of peptidoglycan hydrolases- a new class of antibacterial proteins.
27229861	1	27	from	development	172:182	arg1	bacteria					201:208	bacteria	201:208	bacteria	201:208	BACKGROUND The efficacy of antibiotics against bacterial infections is decreasing due to the development of resistance in bacteria, and thus, there is a need to search for potential alternatives to antibiotics.
27229861	10	28	theme	peptidoglycan	1978:1990	arg1	hydrolases					1992:2001	peptidoglycan hydrolases	1978:2001	peptidoglycan hydrolases	1978:2001	To our knowledge, this is the only tool available for the prediction of peptidoglycan hydrolases from genomic and metagenomic data.
27229861	2	29	theme	gram-negative	478:490	arg1	bacteria					492:499	both gram-positive and gram-negative bacteria	455:499	both gram-positive and gram-negative bacteria	455:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	5	30	theme	Machines	1300:1307	arg1	optimization					1251:1262	optimization	1251:1262	optimization of Random Forest and Support Vector Machines	1251:1307	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	5	30	theme	Machines	1300:1307	arg1	training					1238:1245	training	1238:1245	training	1238:1245	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	4	31	from	classification	1054:1067	arg1	data					1132:1135	genomic and metagenomic data	1108:1135	genomic and metagenomic data	1108:1135	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	2	32	used	used	340:343	arg2	agents					372:377	alternate antibacterial agents	348:377	alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria	348:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	2	32	used	used	340:343	arg2	hydrolases					322:331	peptidoglycan hydrolases	308:331	peptidoglycan hydrolases	308:331	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	9	33	dep	CONCLUSIONS	1753:1763	arg1	helps					1782:1786	helps	1782:1786	helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs	1782:1903	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	7	34	dep	%	1612:1612	arg1	100 					1608:1611	100 	1608:1611	100 	1608:1611	The tool was validated on 24 independent genomic datasets and showed up to 100 % sensitivity and 0.94 MCC.
27229861	7	35	theme	genomic	1574:1580	arg1	datasets					1582:1589	24 independent genomic datasets	1559:1589	24 independent genomic datasets	1559:1589	The tool was validated on 24 independent genomic datasets and showed up to 100 % sensitivity and 0.94 MCC.
27229861	2	36	from	present	444:450	arg1	bacteria					492:499	both gram-positive and gram-negative bacteria	455:499	both gram-positive and gram-negative bacteria	455:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	4	37	theme	tool	1019:1022	arg1	development					988:998	the development	984:998	the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data	984:1135	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	2	38	theme	wall	439:442	arg1	present					444:450	peptidoglycan cell wall present	420:450	peptidoglycan cell wall present in both gram-positive and gram-negative bacteria	420:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	5	39	theme	dipeptide	1204:1212	arg1	composition					1214:1224	dipeptide composition	1204:1224	dipeptide composition	1204:1224	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	6	40	dep	sensitivity	1414:1424	arg1	%					1412:1412	%	1412:1412	%	1412:1412	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	2	41	theme	peptidoglycan	420:432	arg1	present					444:450	peptidoglycan cell wall present	420:450	peptidoglycan cell wall present in both gram-positive and gram-negative bacteria	420:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	9	42	theme	novel	1832:1836	arg1	hydrolases					1852:1861	novel peptidoglycan hydrolases	1832:1861	novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs	1832:1903	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	6	43	theme	99.55 	1448:1453	arg1	%					1454:1454	%	1454:1454	%	1454:1454	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	10	44	from	data	2032:2035	arg1	prediction					1964:1973	the prediction	1960:1973	the prediction of peptidoglycan hydrolases from genomic and metagenomic data	1960:2035	To our knowledge, this is the only tool available for the prediction of peptidoglycan hydrolases from genomic and metagenomic data.
27229861	9	45	from	identification	1795:1808	arg1	ORFs					1900:1903	complete genomic or metagenomic ORFs	1868:1903	complete genomic or metagenomic ORFs	1868:1903	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	1	46	theme	antibiotics	106:116	arg1	efficacy					94:101	The efficacy	90:101	The efficacy of antibiotics against bacterial infections	90:145	BACKGROUND The efficacy of antibiotics against bacterial infections is decreasing due to the development of resistance in bacteria, and thus, there is a need to search for potential alternatives to antibiotics.
27229861	2	47	theme	gram-positive	460:472	arg1	bacteria					492:499	both gram-positive and gram-negative bacteria	455:499	both gram-positive and gram-negative bacteria	455:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	2	48	theme	unique	392:397	arg1	property					399:406	their unique property	386:406	their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria	386:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	9	49	theme	complete	1868:1875	arg1	ORFs					1900:1903	complete genomic or metagenomic ORFs	1868:1903	complete genomic or metagenomic ORFs	1868:1903	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	10	50	theme	available	1946:1954	arg1	this					1924:1927	this	1924:1927	this	1924:1927	To our knowledge, this is the only tool available for the prediction of peptidoglycan hydrolases from genomic and metagenomic data.
27229861	10	50	theme	available	1946:1954	arg1	tool					1941:1944	the only tool	1932:1944	the only tool available for the prediction of peptidoglycan hydrolases from genomic and metagenomic data	1932:2035	To our knowledge, this is the only tool available for the prediction of peptidoglycan hydrolases from genomic and metagenomic data.
27229861	6	51	theme	99.98 	1427:1432	arg1	%					1433:1433	%	1433:1433	%	1433:1433	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	5	52	theme	acid	1195:1198	arg1	features					1226:1233	amino acid and dipeptide composition features	1189:1233	amino acid and dipeptide composition features	1189:1233	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	3	53	from	data	837:840	arg1	hydrolases					797:806	novel peptidoglycan hydrolases	777:806	novel peptidoglycan hydrolases from genomic and metagenomic data	777:840	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	3	53	from	data	837:840	arg1	classification					759:772	classification	759:772	classification	759:772	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	3	53	from	data	837:840	arg1	identification					740:753	identification	740:753	identification	740:753	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	2	54	theme	peptidoglycan	308:320	arg1	hydrolases					322:331	peptidoglycan hydrolases	308:331	peptidoglycan hydrolases	308:331	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	2	54	theme	peptidoglycan	308:320	arg1	agents					372:377	alternate antibacterial agents	348:377	alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria	348:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	5	55	theme	Random	1267:1272	arg1	Forest					1274:1279	Random Forest	1267:1279	Random Forest	1267:1279	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	3	56	theme	bacterial	660:668	arg1	pathophysiology					670:684	bacterial pathophysiology	660:684	bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data	660:840	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	2	57	theme	alternate	348:356	arg1	hydrolases					322:331	peptidoglycan hydrolases	308:331	peptidoglycan hydrolases	308:331	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	2	57	theme	alternate	348:356	arg1	agents					372:377	alternate antibacterial agents	348:377	alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria	348:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	6	58	theme	different	1486:1494	arg1	classes					1496:1502	four different classes	1481:1502	four different classes of peptidoglycan hydrolases	1481:1530	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	3	59	theme	computational	713:725	arg1	tool					727:730	a computational tool	711:730	a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data	711:840	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	8	60	theme	novel	1672:1676	arg1	hydrolases					1692:1701	novel peptidoglycan hydrolases	1672:1701	novel peptidoglycan hydrolases	1672:1701	The ability of HyPe to identify novel peptidoglycan hydrolases was also demonstrated on 24 metagenomic datasets.
27229861	4	61	theme	peptidoglycan	1078:1090	arg1	hydrolases					1092:1101	novel peptidoglycan hydrolases	1072:1101	novel peptidoglycan hydrolases from genomic and metagenomic data	1072:1135	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	62	dep	tool	1019:1022	arg1	HyPe					1025:1028	HyPe	1025:1028	HyPe	1025:1028	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	3	63	theme	overall	535:541	arg1	turnover					557:564	overall peptidoglycan turnover	535:564	overall peptidoglycan turnover	535:564	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	6	64	theme	Random	1310:1315	arg1	model					1335:1339	Random Forest multiclass model	1310:1339	Random Forest multiclass model	1310:1339	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	9	65	from	classification	1814:1827	arg1	ORFs					1900:1903	complete genomic or metagenomic ORFs	1868:1903	complete genomic or metagenomic ORFs	1868:1903	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	1	66	theme	resistance	187:196	arg1	development					172:182	the development	168:182	the development of resistance in bacteria	168:208	BACKGROUND The efficacy of antibiotics against bacterial infections is decreasing due to the development of resistance in bacteria, and thus, there is a need to search for potential alternatives to antibiotics.
27229861	4	67	theme	known	870:874	arg1	RESULTS					843:849	RESULTS	843:849	RESULTS In this study	843:863	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	67	theme	known	870:874	arg1	hydrolases					890:899	the known peptidoglycan hydrolases	866:899	the known peptidoglycan hydrolases	866:899	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	3	68	theme	novel	777:781	arg1	hydrolases					797:806	novel peptidoglycan hydrolases	777:806	novel peptidoglycan hydrolases from genomic and metagenomic data	777:840	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	8	69	theme	metagenomic	1731:1741	arg1	datasets					1743:1750	24 metagenomic datasets	1728:1750	24 metagenomic datasets	1728:1750	The ability of HyPe to identify novel peptidoglycan hydrolases was also demonstrated on 24 metagenomic datasets.
27229861	6	70	theme	HyPe	1377:1380	arg1	tool					1382:1385	HyPe tool	1377:1385	HyPe tool	1377:1385	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	3	71	theme	daughter	583:590	arg1	separation					597:606	daughter cell separation	583:606	daughter cell separation	583:606	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	6	72	theme	hydrolases	1521:1530	arg1	classes					1496:1502	four different classes	1481:1502	four different classes of peptidoglycan hydrolases	1481:1530	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	4	73	from	data	1132:1135	arg1	classification					1054:1067	classification	1054:1067	classification	1054:1067	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	73	from	data	1132:1135	arg1	identification					1035:1048	identification	1035:1048	identification	1035:1048	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	73	from	data	1132:1135	arg1	hydrolases					1092:1101	novel peptidoglycan hydrolases	1072:1101	novel peptidoglycan hydrolases from genomic and metagenomic data	1072:1135	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	8	74	theme	HyPe	1655:1658	arg1	ability					1644:1650	The ability	1640:1650	The ability of HyPe to identify novel peptidoglycan hydrolases	1640:1701	The ability of HyPe to identify novel peptidoglycan hydrolases was also demonstrated on 24 metagenomic datasets.
27229861	3	75	theme	genomic	813:819	arg1	data					837:840	genomic and metagenomic data	813:840	genomic and metagenomic data	813:840	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	9	76	dep	identification	1795:1808	arg1	the					1791:1793	the	1791:1793	the	1791:1793	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	6	77	from	accuracy	1456:1463	arg1	classes					1496:1502	four different classes	1481:1502	four different classes of peptidoglycan hydrolases	1481:1530	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	4	78	theme	multiple	919:926	arg1	classes					928:934	multiple classes	919:934	multiple classes based on their site of action	919:964	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	1	79	dep	BACKGROUND	79:88	arg1	is					227:228	is	227:228	is a need to search for potential alternatives to antibiotics	227:287	BACKGROUND The efficacy of antibiotics against bacterial infections is decreasing due to the development of resistance in bacteria, and thus, there is a need to search for potential alternatives to antibiotics.
27229861	1	79	dep	BACKGROUND	79:88	arg1	decreasing					150:159	decreasing	150:159	is decreasing due to the development of resistance in bacteria	147:208	BACKGROUND The efficacy of antibiotics against bacterial infections is decreasing due to the development of resistance in bacteria, and thus, there is a need to search for potential alternatives to antibiotics.
27229861	3	80	theme	hydrolases	797:806	arg1	classification					759:772	classification	759:772	classification	759:772	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	3	80	theme	hydrolases	797:806	arg1	identification					740:753	identification	740:753	identification	740:753	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	3	81	theme	peptidoglycan	609:621	arg1	hydrolases					623:632	peptidoglycan hydrolases	609:632	peptidoglycan hydrolases	609:632	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	82	from	RESULTS	843:849	arg1	study					859:863	this study	854:863	this study	854:863	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	0	83	theme	proteins	69:76	arg1	class					46:50	a new class	40:50	a new class of antibacterial proteins	40:76	Prediction of peptidoglycan hydrolases- a new class of antibacterial proteins.
27229861	6	84	from	specificity	1435:1445	arg1	classes					1496:1502	four different classes	1481:1502	four different classes of peptidoglycan hydrolases	1481:1530	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	9	85	theme	metagenomic	1888:1898	arg1	ORFs					1900:1903	complete genomic or metagenomic ORFs	1868:1903	complete genomic or metagenomic ORFs	1868:1903	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	9	86	theme	present	1769:1775	arg1	tool					1777:1780	The present tool	1765:1780	The present tool	1765:1780	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	5	87	theme	Vector	1293:1298	arg1	Machines					1300:1307	Support Vector Machines	1285:1307	Support Vector Machines	1285:1307	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	3	88	theme	metagenomic	825:835	arg1	data					837:840	genomic and metagenomic data	813:840	genomic and metagenomic data	813:840	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	6	89	from	sensitivity	1414:1424	arg1	classes					1496:1502	four different classes	1481:1502	four different classes of peptidoglycan hydrolases	1481:1530	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	7	90	theme	independent	1562:1572	arg1	datasets					1582:1589	24 independent genomic datasets	1559:1589	24 independent genomic datasets	1559:1589	The tool was validated on 24 independent genomic datasets and showed up to 100 % sensitivity and 0.94 MCC.
27229861	7	91	dep	sensitivity	1614:1624	arg1	%					1612:1612	%	1612:1612	%	1612:1612	The tool was validated on 24 independent genomic datasets and showed up to 100 % sensitivity and 0.94 MCC.
27229861	6	92	dep	%	1412:1412	arg1	71.12 					1406:1411	71.12 	1406:1411	71.12 	1406:1411	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	3	93	from	classification	759:772	arg1	data					837:840	genomic and metagenomic data	813:840	genomic and metagenomic data	813:840	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	2	94	theme	due	379:381	arg1	hydrolases					322:331	peptidoglycan hydrolases	308:331	peptidoglycan hydrolases	308:331	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	2	94	theme	due	379:381	arg1	agents					372:377	alternate antibacterial agents	348:377	alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria	348:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	5	95	theme	Various	1138:1144	arg1	models					1161:1166	Various classification models	1138:1166	Various classification models	1138:1166	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	2	96	theme	cell	434:437	arg1	present					444:450	peptidoglycan cell wall present	420:450	peptidoglycan cell wall present in both gram-positive and gram-negative bacteria	420:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	2	97	theme	antibacterial	358:370	arg1	hydrolases					322:331	peptidoglycan hydrolases	308:331	peptidoglycan hydrolases	308:331	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	2	97	theme	antibacterial	358:370	arg1	agents					372:377	alternate antibacterial agents	348:377	alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria	348:499	In this scenario, peptidoglycan hydrolases can be used as alternate antibacterial agents due to their unique property of cleaving peptidoglycan cell wall present in both gram-positive and gram-negative bacteria.
27229861	6	98	theme	%	1454:1454	arg1	accuracy					1456:1463	99.55 % accuracy	1448:1463	99.55 % accuracy	1448:1463	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	4	99	from	identification	1035:1048	arg1	data					1132:1135	genomic and metagenomic data	1108:1135	genomic and metagenomic data	1108:1135	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	9	100	theme	peptidoglycan	1838:1850	arg1	hydrolases					1852:1861	novel peptidoglycan hydrolases	1832:1861	novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs	1832:1903	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	3	101	theme	crucial	644:650	arg1	role					652:655	crucial role	644:655	crucial role	644:655	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	0	102	theme	new	42:44	arg1	class					46:50	a new class	40:50	a new class of antibacterial proteins	40:76	Prediction of peptidoglycan hydrolases- a new class of antibacterial proteins.
27229861	4	103	theme	computational	1005:1017	arg1	tool					1019:1022	a computational tool	1003:1022	a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data	1003:1135	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	6	104	theme	%	1433:1433	arg1	specificity					1435:1445	99.98 % specificity	1427:1445	99.98 % specificity	1427:1445	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	5	105	theme	amino	1189:1193	arg1	acid					1195:1198	amino acid	1189:1198	amino acid	1189:1198	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	9	106	theme	genomic	1877:1883	arg1	ORFs					1900:1903	complete genomic or metagenomic ORFs	1868:1903	complete genomic or metagenomic ORFs	1868:1903	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	6	107	dep	71.12 	1406:1411	arg1	to					1403:1404	to	1403:1404	to	1403:1404	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	6	108	theme	peptidoglycan	1507:1519	arg1	hydrolases					1521:1530	peptidoglycan hydrolases	1507:1530	peptidoglycan hydrolases	1507:1530	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	3	109	dep	identification	740:753	arg1	the					736:738	the	736:738	the	736:738	Along with a role in maintaining overall peptidoglycan turnover in a cell and in daughter cell separation, peptidoglycan hydrolases also play crucial role in bacterial pathophysiology requiring development of a computational tool for the identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	6	110	from	MCC	1474:1476	arg1	classes					1496:1502	four different classes	1481:1502	four different classes of peptidoglycan hydrolases	1481:1530	Random Forest multiclass model was selected for the development of HyPe tool as it showed up to 71.12 % sensitivity, 99.98 % specificity, 99.55 % accuracy and 0.80 MCC in four different classes of peptidoglycan hydrolases.
27229861	4	111	theme	hydrolases	1092:1101	arg1	classification					1054:1067	classification	1054:1067	classification	1054:1067	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	4	111	theme	hydrolases	1092:1101	arg1	identification					1035:1048	identification	1035:1048	identification	1035:1048	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	9	112	theme	hydrolases	1852:1861	arg1	classification					1814:1827	classification	1814:1827	classification	1814:1827	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	9	112	theme	hydrolases	1852:1861	arg1	identification					1795:1808	identification	1795:1808	identification	1795:1808	CONCLUSIONS The present tool helps in the identification and classification of novel peptidoglycan hydrolases from complete genomic or metagenomic ORFs.
27229861	5	113	theme	Forest	1274:1279	arg1	optimization					1251:1262	optimization	1251:1262	optimization of Random Forest and Support Vector Machines	1251:1307	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	5	113	theme	Forest	1274:1279	arg1	training					1238:1245	training	1238:1245	training	1238:1245	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
27229861	4	114	theme	genomic	1108:1114	arg1	data					1132:1135	genomic and metagenomic data	1108:1135	genomic and metagenomic data	1108:1135	RESULTS In this study, the known peptidoglycan hydrolases were divided into multiple classes based on their site of action and were used for the development of a computational tool 'HyPe' for identification and classification of novel peptidoglycan hydrolases from genomic and metagenomic data.
27229861	11	115	dep	//metabiosys.iiserb.ac.in/hype/	2099:2129	arg1	//metagenomics.iiserb.ac.in/hype/					2056:2088	//metagenomics.iiserb.ac.in/hype/	2056:2088	//metagenomics.iiserb.ac.in/hype/	2056:2088	AVAILABILITY http://metagenomics.iiserb.ac.in/hype/ and http://metabiosys.iiserb.ac.in/hype/ .
27229861	11	115	dep	//metabiosys.iiserb.ac.in/hype/	2099:2129	arg1	http					2051:2054	AVAILABILITY http	2038:2054	AVAILABILITY http	2038:2054	AVAILABILITY http://metagenomics.iiserb.ac.in/hype/ and http://metabiosys.iiserb.ac.in/hype/ .
27229861	11	115	dep	//metabiosys.iiserb.ac.in/hype/	2099:2129	arg1	http					2094:2097	http	2094:2097	http	2094:2097	AVAILABILITY http://metagenomics.iiserb.ac.in/hype/ and http://metabiosys.iiserb.ac.in/hype/ .
27229861	10	116	theme	only	1936:1939	arg1	this					1924:1927	this	1924:1927	this	1924:1927	To our knowledge, this is the only tool available for the prediction of peptidoglycan hydrolases from genomic and metagenomic data.
27229861	10	116	theme	only	1936:1939	arg1	tool					1941:1944	the only tool	1932:1944	the only tool available for the prediction of peptidoglycan hydrolases from genomic and metagenomic data	1932:2035	To our knowledge, this is the only tool available for the prediction of peptidoglycan hydrolases from genomic and metagenomic data.
27229861	8	117	theme	peptidoglycan	1678:1690	arg1	hydrolases					1692:1701	novel peptidoglycan hydrolases	1672:1701	novel peptidoglycan hydrolases	1672:1701	The ability of HyPe to identify novel peptidoglycan hydrolases was also demonstrated on 24 metagenomic datasets.
27229861	5	118	theme	Support	1285:1291	arg1	Machines					1300:1307	Support Vector Machines	1285:1307	Support Vector Machines	1285:1307	Various classification models were developed using amino acid and dipeptide composition features by training and optimization of Random Forest and Support Vector Machines.
25381909	7	0	theme	similar	1218:1224	arg1	fashion					1226:1232	a similar fashion	1216:1232	a similar fashion	1216:1232	Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion.
25381909	6	1	dep	several	894:900	arg1	concerns					902:909	concerns	902:909	concerns	902:909	This raises several concerns for using this reagent to treat large preparations of therapeutic proteins generated in P. pastoris.
25381909	1	2	theme	methylotrophic	101:114	arg1	system					166:171	an attractive expression system	141:171	an attractive expression system due to its ability to secrete large amounts of recombinant protein	141:238	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	1	2	theme	methylotrophic	101:114	arg1	pastoris					129:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	4	3	theme	O-linked	640:647	arg1	mannose					649:655	O-linked mannose	640:655	O-linked mannose	640:655	Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris.
25381909	1	4	theme	due	173:175	arg1	system					166:171	an attractive expression system	141:171	an attractive expression system due to its ability to secrete large amounts of recombinant protein	141:238	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	1	4	theme	due	173:175	arg1	pastoris					129:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	9	5	link	O-linked	1591:1598	arg1	processing					1608:1617	O-linked mannose processing	1591:1617	O-linked mannose processing	1591:1617	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	0	6	link	O-linked	25:32	arg1	removal					42:48	O-linked mannose removal	25:48	O-linked mannose removal	25:48	A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase.
25381909	4	7	theme	bean	603:606	arg1	α-1,2/3/6-mannosidase					608:628	jack bean α-1,2/3/6-mannosidase	598:628	jack bean α-1,2/3/6-mannosidase	598:628	Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris.
25381909	9	8	theme	several	1667:1673	arg1	benefits					1675:1682	several benefits	1667:1682	several benefits	1667:1682	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	1	9	theme	large	203:207	arg1	amounts					209:215	large amounts	203:215	large amounts of recombinant protein	203:238	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	1	9	theme	large	203:207	arg1	protein					232:238	recombinant protein	220:238	recombinant protein	220:238	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	4	10	link	O-linked	640:647	arg1	mannose					649:655	O-linked mannose	640:655	O-linked mannose	640:655	Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris.
25381909	4	11	theme	glycoengineered	707:721	arg1	pastoris					726:733	glycoengineered P. pastoris	707:733	glycoengineered P. pastoris	707:733	Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris.
25381909	1	12	theme	yeast	116:120	arg1	system					166:171	an attractive expression system	141:171	an attractive expression system due to its ability to secrete large amounts of recombinant protein	141:238	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	1	12	theme	yeast	116:120	arg1	pastoris					129:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	8	13	theme	glycosidic	1461:1470	arg1	bond					1472:1475	the Man-α-O-Ser/Thr glycosidic bond	1441:1475	the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins	1441:1499	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	2	14	from	Advances	279:286	arg1	glycoengineering					291:306	glycoengineering	291:306	glycoengineering of P. pastoris	291:321	Advances in glycoengineering of P. pastoris have successfully demonstrated the humanization of both the N- and O-linked glycosylation pathways in this organism.
25381909	6	15	theme	P.	999:1000	arg1	pastoris					1002:1009	P. pastoris	999:1009	P. pastoris	999:1009	This raises several concerns for using this reagent to treat large preparations of therapeutic proteins generated in P. pastoris.
25381909	9	16	theme	crude	1700:1704	arg1	extracts					1728:1735	crude jack bean mannosidase extracts	1700:1735	crude jack bean mannosidase extracts	1700:1735	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	8	17	theme	lysosomal	1283:1291	arg1	mannosidases					1293:1304	recombinant lysosomal mannosidases	1271:1304	recombinant lysosomal mannosidases from different organisms	1271:1329	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	7	18	link	N-linked	1160:1167	arg1	glycans					1169:1175	N-linked glycans	1160:1175	N-linked glycans	1160:1175	Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion.
25381909	5	19	theme	crude	857:861	arg1	extracts					872:879	crude cellular extracts	857:879	crude cellular extracts	857:879	However, one caveat of this strategy is that jack bean mannosidase has yet to be cloned and as such is only available as crude cellular extracts.
25381909	9	20	theme	bean	1711:1714	arg1	extracts					1728:1735	crude jack bean mannosidase extracts	1700:1735	crude jack bean mannosidase extracts	1700:1735	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	5	21	theme	strategy	764:771	arg1	caveat					749:754	one caveat	745:754	one caveat of this strategy	745:771	However, one caveat of this strategy is that jack bean mannosidase has yet to be cloned and as such is only available as crude cellular extracts.
25381909	1	22	theme	expression	155:164	arg1	system					166:171	an attractive expression system	141:171	an attractive expression system due to its ability to secrete large amounts of recombinant protein	141:238	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	1	22	theme	expression	155:164	arg1	pastoris					129:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	6	23	theme	therapeutic	965:975	arg1	proteins					977:984	therapeutic proteins	965:984	therapeutic proteins generated in P. pastoris	965:1009	This raises several concerns for using this reagent to treat large preparations of therapeutic proteins generated in P. pastoris.
25381909	9	24	theme	extracts	1728:1735	arg1	use					1693:1695	the use	1689:1695	the use of crude jack bean mannosidase extracts	1689:1735	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	0	25	theme	lysosomal	74:82	arg1	mannosidase					84:94	recombinant lysosomal mannosidase	62:94	recombinant lysosomal mannosidase	62:94	A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase.
25381909	9	26	dep	show	1526:1529	arg1	only					1515:1518	only	1515:1518	only	1515:1518	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	2	27	theme	O-linked	390:397	arg1	glycosylation					399:411	O-linked glycosylation	390:411	O-linked glycosylation	390:411	Advances in glycoengineering of P. pastoris have successfully demonstrated the humanization of both the N- and O-linked glycosylation pathways in this organism.
25381909	8	28	link	O-linked	1388:1395	arg1	chains					1405:1410	extended O-linked mannose chains	1379:1410	extended O-linked mannose chains	1379:1410	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	2	29	theme	N-	383:384	arg1	pathways					413:420	both the N- and O-linked glycosylation pathways	374:420	pathways	413:420	Advances in glycoengineering of P. pastoris have successfully demonstrated the humanization of both the N- and O-linked glycosylation pathways in this organism.
25381909	7	30	theme	N-linked	1160:1167	arg1	glycans					1169:1175	N-linked glycans	1160:1175	N-linked glycans	1160:1175	Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion.
25381909	4	31	theme	undenatured	669:679	arg1	glycoproteins					681:693	intact undenatured glycoproteins	662:693	intact undenatured glycoproteins produced in glycoengineered P. pastoris	662:733	Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris.
25381909	8	32	theme	extended	1379:1386	arg1	chains					1405:1410	extended O-linked mannose chains	1379:1410	extended O-linked mannose chains	1379:1410	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	2	33	theme	pathways	413:420	arg1	humanization					358:369	the humanization	354:369	the humanization of both the N- and O-linked glycosylation pathways in this organism	354:437	Advances in glycoengineering of P. pastoris have successfully demonstrated the humanization of both the N- and O-linked glycosylation pathways in this organism.
25381909	0	34	theme	O-linked	25:32	arg1	removal					42:48	O-linked mannose removal	25:48	O-linked mannose removal	25:48	A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase.
25381909	7	35	theme	jack	1135:1138	arg1	mannosidase					1145:1155	jack bean mannosidase	1135:1155	jack bean mannosidase	1135:1155	Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion.
25381909	9	36	theme	lysosomal	1565:1573	arg1	mannosidase					1575:1585	lysosomal mannosidase	1565:1585	lysosomal mannosidase	1565:1585	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	8	37	theme	mannose	1397:1403	arg1	chains					1405:1410	extended O-linked mannose chains	1379:1410	extended O-linked mannose chains	1379:1410	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	5	38	theme	bean	786:789	arg1	mannosidase					791:801	jack bean mannosidase	781:801	jack bean mannosidase	781:801	However, one caveat of this strategy is that jack bean mannosidase has yet to be cloned and as such is only available as crude cellular extracts.
25381909	9	39	contain	has	1663:1665	arg1	it					1660:1661	it	1660:1661	it	1660:1661	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	9	39	contain	has	1663:1665	arg2	benefits					1675:1682	several benefits	1667:1682	several benefits	1667:1682	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	9	40	theme	mannose	1600:1606	arg1	processing					1608:1617	O-linked mannose processing	1591:1617	O-linked mannose processing	1591:1617	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	4	41	theme	intact	662:667	arg1	glycoproteins					681:693	intact undenatured glycoproteins	662:693	intact undenatured glycoproteins produced in glycoengineered P. pastoris	662:733	Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris.
25381909	4	42	theme	in	578:579	arg1	utility					587:593	the in vitro utility	574:593	the in vitro utility of jack bean α-1,2/3/6-mannosidase	574:628	Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris.
25381909	2	43	theme	glycosylation	399:411	arg1	pathways					413:420	both the N- and O-linked glycosylation pathways	374:420	pathways	413:420	Advances in glycoengineering of P. pastoris have successfully demonstrated the humanization of both the N- and O-linked glycosylation pathways in this organism.
25381909	1	44	theme	recombinant	220:230	arg1	protein					232:238	recombinant protein	220:238	recombinant protein	220:238	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	7	45	theme	similar	1113:1119	arg1	activities					1121:1130	similar activities	1113:1130	similar activities	1113:1130	Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion.
25381909	3	46	attach	presence	471:478	arg1	protein					517:523	a therapeutic protein	503:523	a therapeutic protein	503:523	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	3	46	attach	presence	471:478	arg2	glycans					492:498	O-linked glycans	483:498	O-linked glycans	483:498	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	7	47	theme	O-linked	1196:1203	arg1	glycans					1205:1211	O-linked glycans	1196:1211	O-linked glycans	1196:1211	Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion.
25381909	3	48	theme	glycans	492:498	arg1	presence					471:478	the presence	467:478	the presence of O-linked glycans on a therapeutic protein	467:523	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	3	48	theme	glycans	492:498	arg1	desirable					536:544	desirable	536:544	desirable	536:544	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	5	49	theme	cellular	863:870	arg1	extracts					872:879	crude cellular extracts	857:879	crude cellular extracts	857:879	However, one caveat of this strategy is that jack bean mannosidase has yet to be cloned and as such is only available as crude cellular extracts.
25381909	9	50	theme	recombinant	1640:1650	arg1	this					1630:1633	this	1630:1633	this	1630:1633	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	9	50	theme	recombinant	1640:1650	arg1	enzyme					1652:1657	a recombinant enzyme	1638:1657	a recombinant enzyme	1638:1657	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	2	51	from	humanization	358:369	arg1	organism					430:437	this organism	425:437	this organism	425:437	Advances in glycoengineering of P. pastoris have successfully demonstrated the humanization of both the N- and O-linked glycosylation pathways in this organism.
25381909	3	52	from	presence	471:478	arg1	protein					517:523	a therapeutic protein	503:523	a therapeutic protein	503:523	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	7	53	link	O-linked	1196:1203	arg1	glycans					1205:1211	O-linked glycans	1196:1211	O-linked glycans	1196:1211	Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion.
25381909	6	54	theme	large	943:947	arg1	preparations					949:960	large preparations	943:960	large preparations of therapeutic proteins generated in P. pastoris	943:1009	This raises several concerns for using this reagent to treat large preparations of therapeutic proteins generated in P. pastoris.
25381909	7	55	theme	lysosomal	1042:1050	arg1	mannosidases					1052:1063	lysosomal mannosidases	1042:1063	lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans	1042:1175	Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion.
25381909	8	56	theme	Man-α-O-Ser/Thr	1445:1459	arg1	bond					1472:1475	the Man-α-O-Ser/Thr glycosidic bond	1441:1475	the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins	1441:1499	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	4	57	theme	α-1,2/3/6-mannosidase	608:628	arg1	utility					587:593	the in vitro utility	574:593	the in vitro utility of jack bean α-1,2/3/6-mannosidase	574:628	Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris.
25381909	8	58	gly	glycoproteins	1487:1499	arg1	glycoproteins					1487:1499	intact glycoproteins	1480:1499	intact glycoproteins	1480:1499	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	3	59	from	desirable	536:544	arg1	cases					460:464	certain cases	452:464	certain cases	452:464	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	4	60	gly	glycoproteins	681:693	arg1	glycoproteins					681:693	intact undenatured glycoproteins	662:693	intact undenatured glycoproteins produced in glycoengineered P. pastoris	662:733	Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris.
25381909	1	61	theme	Pichia	122:127	arg1	system					166:171	an attractive expression system	141:171	an attractive expression system due to its ability to secrete large amounts of recombinant protein	141:238	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	1	61	theme	Pichia	122:127	arg1	pastoris					129:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	3	62	link	O-linked	483:490	arg1	glycans					492:498	O-linked glycans	483:498	O-linked glycans	483:498	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	8	63	theme	intact	1480:1485	arg1	glycoproteins					1487:1499	intact glycoproteins	1480:1499	intact glycoproteins	1480:1499	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	4	64	theme	P.	723:724	arg1	pastoris					726:733	glycoengineered P. pastoris	707:733	glycoengineered P. pastoris	707:733	Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris.
25381909	4	65	dep	in	578:579	arg1	vitro					581:585	vitro	581:585	vitro	581:585	Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris.
25381909	8	66	theme	recombinant	1271:1281	arg1	mannosidases					1293:1304	recombinant lysosomal mannosidases	1271:1304	recombinant lysosomal mannosidases from different organisms	1271:1329	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	0	67	theme	recombinant	62:72	arg1	mannosidase					84:94	recombinant lysosomal mannosidase	62:94	recombinant lysosomal mannosidase	62:94	A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase.
25381909	1	68	theme	attractive	144:153	arg1	system					166:171	an attractive expression system	141:171	an attractive expression system due to its ability to secrete large amounts of recombinant protein	141:238	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	1	68	theme	attractive	144:153	arg1	pastoris					129:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris	97:136	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	6	69	theme	proteins	977:984	arg1	preparations					949:960	large preparations	943:960	large preparations of therapeutic proteins generated in P. pastoris	943:1009	This raises several concerns for using this reagent to treat large preparations of therapeutic proteins generated in P. pastoris.
25381909	9	70	theme	mannosidase	1716:1726	arg1	extracts					1728:1735	crude jack bean mannosidase extracts	1700:1735	crude jack bean mannosidase extracts	1700:1735	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	8	71	theme	mannosidases	1293:1304	arg1	panel					1262:1266	a panel	1260:1266	a panel of recombinant lysosomal mannosidases from different organisms	1260:1329	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	7	72	contain	have	1108:1111	arg2	activities					1121:1130	similar activities	1113:1130	similar activities	1113:1130	Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion.
25381909	7	72	contain	have	1108:1111	arg1	mannosidases					1052:1063	lysosomal mannosidases	1042:1063	lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans	1042:1175	Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion.
25381909	0	73	theme	mannosidase	84:94	arg1	use					55:57	the use	51:57	A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase.	0:95	A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase.
25381909	0	74	dep	approach	12:19	arg1	use					55:57	the use	51:57	A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase.	0:95	A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase.
25381909	8	75	from	panel	1262:1266	arg1	organisms					1321:1329	different organisms	1311:1329	different organisms	1311:1329	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	8	76	theme	different	1311:1319	arg1	organisms					1321:1329	different organisms	1311:1329	different organisms	1311:1329	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	8	77	from	bond	1472:1475	arg1	glycoproteins					1487:1499	intact glycoproteins	1480:1499	intact glycoproteins	1480:1499	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	0	78	theme	practical	2:10	arg1	approach					12:19	A practical approach	0:19	A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase.	0:95	A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase.
25381909	9	79	theme	first	1539:1543	arg1	time					1545:1548	the first time	1535:1548	the first time	1535:1548	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	7	80	theme	bean	1140:1143	arg1	mannosidase					1145:1155	jack bean mannosidase	1135:1155	jack bean mannosidase	1135:1155	Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion.
25381909	0	81	theme	mannose	34:40	arg1	removal					42:48	O-linked mannose removal	25:48	O-linked mannose removal	25:48	A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase.
25381909	2	82	link	O-linked	390:397	arg1	glycosylation					399:411	O-linked glycosylation	390:411	O-linked glycosylation	390:411	Advances in glycoengineering of P. pastoris have successfully demonstrated the humanization of both the N- and O-linked glycosylation pathways in this organism.
25381909	3	83	theme	certain	452:458	arg1	cases					460:464	certain cases	452:464	certain cases	452:464	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	5	84	theme	jack	781:784	arg1	mannosidase					791:801	jack bean mannosidase	781:801	jack bean mannosidase	781:801	However, one caveat of this strategy is that jack bean mannosidase has yet to be cloned and as such is only available as crude cellular extracts.
25381909	9	85	theme	mannosidase	1575:1585	arg1	utility					1554:1560	the utility	1550:1560	the utility of lysosomal mannosidase for O-linked mannose processing	1550:1617	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	8	86	theme	O-linked	1388:1395	arg1	chains					1405:1410	extended O-linked mannose chains	1379:1410	extended O-linked mannose chains	1379:1410	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	3	87	from	cases	460:464	arg1	presence					471:478	the presence	467:478	the presence of O-linked glycans on a therapeutic protein	467:523	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	3	87	from	cases	460:464	arg1	desirable					536:544	desirable	536:544	desirable	536:544	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	3	88	theme	O-linked	483:490	arg1	glycans					492:498	O-linked glycans	483:498	O-linked glycans	483:498	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	1	89	with	system	166:171	arg1	potential					250:258	the potential	246:258	the potential for glycosylation	246:276	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	9	90	theme	O-linked	1591:1598	arg1	processing					1608:1617	O-linked mannose processing	1591:1617	O-linked mannose processing	1591:1617	As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
25381909	2	91	theme	pastoris	314:321	arg1	glycoengineering					291:306	glycoengineering	291:306	glycoengineering of P. pastoris	291:321	Advances in glycoengineering of P. pastoris have successfully demonstrated the humanization of both the N- and O-linked glycosylation pathways in this organism.
25381909	8	92	from	organisms	1321:1329	arg1	panel					1262:1266	a panel	1260:1266	a panel of recombinant lysosomal mannosidases from different organisms	1260:1329	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	8	92	from	organisms	1321:1329	arg1	mannosidases					1293:1304	recombinant lysosomal mannosidases	1271:1304	recombinant lysosomal mannosidases from different organisms	1271:1329	To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins.
25381909	3	93	theme	therapeutic	505:515	arg1	protein					517:523	a therapeutic protein	503:523	a therapeutic protein	503:523	However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable.
25381909	1	94	theme	protein	232:238	arg1	amounts					209:215	large amounts	203:215	large amounts of recombinant protein	203:238	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25381909	1	94	theme	protein	232:238	arg1	protein					232:238	recombinant protein	220:238	recombinant protein	220:238	The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation.
25439895	6	0	theme	Antibacterial	838:850	arg1	experiment					852:861	Antibacterial experiment	838:861	Antibacterial experiment	838:861	Antibacterial experiment revealed excellent antibacterial activity of the cellulose nanocomposite fibers against Staphylococcus aureus.
25439895	6	1	theme	fibers	936:941	arg1	activity					896:903	excellent antibacterial activity	872:903	excellent antibacterial activity of the cellulose nanocomposite fibers against Staphylococcus aureus	872:971	Antibacterial experiment revealed excellent antibacterial activity of the cellulose nanocomposite fibers against Staphylococcus aureus.
25439895	3	2	dep	nm	587:588	arg1	to					590:591	to	590:591	to	590:591	The content and diameter of the Ag nanoparticles (NPs) increased from 0.15% to 2.40% and from 7.2 nm to 12.5 nm, respectively.
25439895	4	3	from	state	673:677	arg1	pores					637:641	the pores	633:641	the pores of the cellulose fiber at wet state	633:677	In our findings, the pores of the cellulose fiber at wet state were used as a microreactor to synthesize Ag nanopariticles.
25439895	4	3	from	state	673:677	arg1	microreactor					694:705	a microreactor	692:705	a microreactor	692:705	In our findings, the pores of the cellulose fiber at wet state were used as a microreactor to synthesize Ag nanopariticles.
25439895	5	4	theme	thermal	819:825	arg1	stability					827:835	thermal stability	819:835	thermal stability	819:835	The cellulose/Ag nanocomposite fibers exhibited good mechanical properties and thermal stability.
25439895	2	5	theme	composite	287:295	arg1	fibers					297:302	the composite fibers	283:302	the composite fibers	283:302	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	3	6	theme	Ag	521:522	arg1	NPs					539:541	NPs	539:541	NPs	539:541	The content and diameter of the Ag nanoparticles (NPs) increased from 0.15% to 2.40% and from 7.2 nm to 12.5 nm, respectively.
25439895	3	6	theme	Ag	521:522	arg1	nanoparticles					524:536	the Ag nanoparticles	517:536	the Ag nanoparticles (NPs)	517:542	The content and diameter of the Ag nanoparticles (NPs) increased from 0.15% to 2.40% and from 7.2 nm to 12.5 nm, respectively.
25439895	7	7	theme	easy	1094:1097	arg1	industrialization					1099:1115	easy industrialization	1094:1115	easy industrialization	1094:1115	The cellulose/silver nanocomposite fibers would have great potential in antibacterial textile and wound handling due to easy industrialization.
25439895	4	8	theme	fiber	660:664	arg1	pores					637:641	the pores	633:641	the pores of the cellulose fiber at wet state	633:677	In our findings, the pores of the cellulose fiber at wet state were used as a microreactor to synthesize Ag nanopariticles.
25439895	4	8	theme	fiber	660:664	arg1	microreactor					694:705	a microreactor	692:705	a microreactor	692:705	In our findings, the pores of the cellulose fiber at wet state were used as a microreactor to synthesize Ag nanopariticles.
25439895	2	9	theme	FT-IR	326:330	arg1	XRD					352:354	XRD	352:354	XRD	352:354	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	2	9	theme	FT-IR	326:330	arg1	diffraction					339:349	FT-IR, X-ray diffraction	326:349	diffraction	339:349	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	3	10	dep	content	493:499	arg1	The					489:491	The	489:491	The	489:491	The content and diameter of the Ag nanoparticles (NPs) increased from 0.15% to 2.40% and from 7.2 nm to 12.5 nm, respectively.
25439895	1	11	theme	cellulose/silver	81:96	arg1	fibers					112:117	cellulose/silver nanocomposite fibers	81:117	cellulose/silver nanocomposite fibers	81:117	We prepared cellulose/silver nanocomposite fibers by soaking the cellulose fibers in AgNO3 aqueous solution, which was heated at 80°C for 24h to synthesize Ag nanoparticles in situ.
25439895	5	12	theme	nanocomposite	757:769	arg1	fibers					771:776	The cellulose/Ag nanocomposite fibers	740:776	The cellulose/Ag nanocomposite fibers	740:776	The cellulose/Ag nanocomposite fibers exhibited good mechanical properties and thermal stability.
25439895	2	13	theme	tensile	472:478	arg1	testing					480:486	tensile testing	472:486	tensile testing	472:486	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	1	14	theme	nanocomposite	98:110	arg1	fibers					112:117	cellulose/silver nanocomposite fibers	81:117	cellulose/silver nanocomposite fibers	81:117	We prepared cellulose/silver nanocomposite fibers by soaking the cellulose fibers in AgNO3 aqueous solution, which was heated at 80°C for 24h to synthesize Ag nanoparticles in situ.
25439895	3	15	theme	nanoparticles	524:536	arg1	diameter					505:512	diameter	505:512	diameter	505:512	The content and diameter of the Ag nanoparticles (NPs) increased from 0.15% to 2.40% and from 7.2 nm to 12.5 nm, respectively.
25439895	3	15	theme	nanoparticles	524:536	arg1	content					493:499	content	493:499	content	493:499	The content and diameter of the Ag nanoparticles (NPs) increased from 0.15% to 2.40% and from 7.2 nm to 12.5 nm, respectively.
25439895	7	16	contain	have	1022:1025	arg1	fibers					1009:1014	The cellulose/silver nanocomposite fibers	974:1014	The cellulose/silver nanocomposite fibers	974:1014	The cellulose/silver nanocomposite fibers would have great potential in antibacterial textile and wound handling due to easy industrialization.
25439895	7	16	contain	have	1022:1025	arg2	potential					1033:1041	great potential	1027:1041	great potential	1027:1041	The cellulose/silver nanocomposite fibers would have great potential in antibacterial textile and wound handling due to easy industrialization.
25439895	2	17	theme	electron	367:374	arg1	microscopy					376:385	scanning electron microscopy	358:385	scanning electron microscopy (SEM)	358:391	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	2	17	theme	electron	367:374	arg1	SEM					388:390	SEM	388:390	SEM	388:390	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	6	18	theme	nanocomposite	922:934	arg1	fibers					936:941	the cellulose nanocomposite fibers	908:941	the cellulose nanocomposite fibers	908:941	Antibacterial experiment revealed excellent antibacterial activity of the cellulose nanocomposite fibers against Staphylococcus aureus.
25439895	7	19	theme	wound	1072:1076	arg1	handling					1078:1085	wound handling	1072:1085	wound handling	1072:1085	The cellulose/silver nanocomposite fibers would have great potential in antibacterial textile and wound handling due to easy industrialization.
25439895	2	20	dep	structure	255:263	arg1	The					251:253	The	251:253	The	251:253	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	6	21	theme	cellulose	912:920	arg1	fibers					936:941	the cellulose nanocomposite fibers	908:941	the cellulose nanocomposite fibers	908:941	Antibacterial experiment revealed excellent antibacterial activity of the cellulose nanocomposite fibers against Staphylococcus aureus.
25439895	1	22	from	fibers	144:149	arg1	solution					168:175	AgNO3 aqueous solution	154:175	AgNO3 aqueous solution	154:175	We prepared cellulose/silver nanocomposite fibers by soaking the cellulose fibers in AgNO3 aqueous solution, which was heated at 80°C for 24h to synthesize Ag nanoparticles in situ.
25439895	2	23	dep	diffraction	339:349	arg1	X-ray					333:337	FT-IR, X-ray diffraction	326:349	X-ray	333:337	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	2	24	theme	transmitting	394:405	arg1	TEM					428:430	TEM	428:430	TEM	428:430	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	2	24	theme	transmitting	394:405	arg1	microscopy					416:425	transmitting electron microscopy	394:425	transmitting electron microscopy (TEM)	394:431	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	2	25	theme	electron	407:414	arg1	TEM					428:430	TEM	428:430	TEM	428:430	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	2	25	theme	electron	407:414	arg1	microscopy					416:425	transmitting electron microscopy	394:425	transmitting electron microscopy (TEM)	394:431	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	2	26	theme	fibers	297:302	arg1	properties					269:278	properties	269:278	properties	269:278	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	2	26	theme	fibers	297:302	arg1	structure					255:263	structure	255:263	structure	255:263	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	3	27	dep	%	563:563	arg1	to					565:566	to	565:566	to	565:566	The content and diameter of the Ag nanoparticles (NPs) increased from 0.15% to 2.40% and from 7.2 nm to 12.5 nm, respectively.
25439895	3	28	theme	7.2	583:585	arg1	nm					587:588	nm	587:588	nm	587:588	The content and diameter of the Ag nanoparticles (NPs) increased from 0.15% to 2.40% and from 7.2 nm to 12.5 nm, respectively.
25439895	2	29	theme	scanning	358:365	arg1	microscopy					376:385	scanning electron microscopy	358:385	scanning electron microscopy (SEM)	358:391	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	2	29	theme	scanning	358:365	arg1	SEM					388:390	SEM	388:390	SEM	388:390	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	0	30	theme	cellulose/silver	30:45	arg1	properties					16:25	properties	16:25	properties	16:25	Preparation and properties of cellulose/silver nanocomposite fibers.
25439895	0	30	theme	cellulose/silver	30:45	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of cellulose/silver nanocomposite fibers.
25439895	2	31	theme	thermo-gravimetric	434:451	arg1	TGA					463:465	TGA	463:465	TGA	463:465	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	2	31	theme	thermo-gravimetric	434:451	arg1	analysis					453:460	thermo-gravimetric analysis	434:460	thermo-gravimetric analysis (TGA)	434:466	The structure and properties of the composite fibers were characterized by FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM), transmitting electron microscopy (TEM), thermo-gravimetric analysis (TGA) and tensile testing.
25439895	7	32	theme	cellulose/silver	978:993	arg1	fibers					1009:1014	The cellulose/silver nanocomposite fibers	974:1014	The cellulose/silver nanocomposite fibers	974:1014	The cellulose/silver nanocomposite fibers would have great potential in antibacterial textile and wound handling due to easy industrialization.
25439895	5	33	theme	cellulose/Ag	744:755	arg1	fibers					771:776	The cellulose/Ag nanocomposite fibers	740:776	The cellulose/Ag nanocomposite fibers	740:776	The cellulose/Ag nanocomposite fibers exhibited good mechanical properties and thermal stability.
25439895	5	34	theme	mechanical	793:802	arg1	properties					804:813	good mechanical properties	788:813	good mechanical properties	788:813	The cellulose/Ag nanocomposite fibers exhibited good mechanical properties and thermal stability.
25439895	4	35	theme	Ag	721:722	arg1	nanopariticles					724:737	Ag nanopariticles	721:737	Ag nanopariticles	721:737	In our findings, the pores of the cellulose fiber at wet state were used as a microreactor to synthesize Ag nanopariticles.
25439895	7	36	theme	nanocomposite	995:1007	arg1	fibers					1009:1014	The cellulose/silver nanocomposite fibers	974:1014	The cellulose/silver nanocomposite fibers	974:1014	The cellulose/silver nanocomposite fibers would have great potential in antibacterial textile and wound handling due to easy industrialization.
25439895	1	37	theme	cellulose	134:142	arg1	fibers					144:149	the cellulose fibers	130:149	the cellulose fibers in AgNO3 aqueous solution, which was heated at 80°C for 24h to synthesize Ag nanoparticles in situ	130:248	We prepared cellulose/silver nanocomposite fibers by soaking the cellulose fibers in AgNO3 aqueous solution, which was heated at 80°C for 24h to synthesize Ag nanoparticles in situ.
25439895	6	38	theme	excellent	872:880	arg1	activity					896:903	excellent antibacterial activity	872:903	excellent antibacterial activity of the cellulose nanocomposite fibers against Staphylococcus aureus	872:971	Antibacterial experiment revealed excellent antibacterial activity of the cellulose nanocomposite fibers against Staphylococcus aureus.
25439895	7	39	theme	great	1027:1031	arg1	potential					1033:1041	great potential	1027:1041	great potential	1027:1041	The cellulose/silver nanocomposite fibers would have great potential in antibacterial textile and wound handling due to easy industrialization.
25439895	3	40	theme	12.5	593:596	arg1	nm					587:588	nm	587:588	nm	587:588	The content and diameter of the Ag nanoparticles (NPs) increased from 0.15% to 2.40% and from 7.2 nm to 12.5 nm, respectively.
25439895	5	41	theme	good	788:791	arg1	properties					804:813	good mechanical properties	788:813	good mechanical properties	788:813	The cellulose/Ag nanocomposite fibers exhibited good mechanical properties and thermal stability.
25439895	4	42	theme	cellulose	650:658	arg1	fiber					660:664	the cellulose fiber	646:664	the cellulose fiber	646:664	In our findings, the pores of the cellulose fiber at wet state were used as a microreactor to synthesize Ag nanopariticles.
25439895	1	43	theme	Ag	225:226	arg1	nanoparticles					228:240	Ag nanoparticles	225:240	Ag nanoparticles	225:240	We prepared cellulose/silver nanocomposite fibers by soaking the cellulose fibers in AgNO3 aqueous solution, which was heated at 80°C for 24h to synthesize Ag nanoparticles in situ.
25439895	4	44	theme	wet	669:671	arg1	state					673:677	wet state	669:677	wet state	669:677	In our findings, the pores of the cellulose fiber at wet state were used as a microreactor to synthesize Ag nanopariticles.
25439895	3	45	theme	2.40	568:571	arg1	%					563:563	%	563:563	%	563:563	The content and diameter of the Ag nanoparticles (NPs) increased from 0.15% to 2.40% and from 7.2 nm to 12.5 nm, respectively.
25439895	1	46	theme	AgNO3	154:158	arg1	solution					168:175	AgNO3 aqueous solution	154:175	AgNO3 aqueous solution	154:175	We prepared cellulose/silver nanocomposite fibers by soaking the cellulose fibers in AgNO3 aqueous solution, which was heated at 80°C for 24h to synthesize Ag nanoparticles in situ.
25439895	3	47	theme	0.15	559:562	arg1	%					563:563	%	563:563	%	563:563	The content and diameter of the Ag nanoparticles (NPs) increased from 0.15% to 2.40% and from 7.2 nm to 12.5 nm, respectively.
25439895	1	48	theme	aqueous	160:166	arg1	solution					168:175	AgNO3 aqueous solution	154:175	AgNO3 aqueous solution	154:175	We prepared cellulose/silver nanocomposite fibers by soaking the cellulose fibers in AgNO3 aqueous solution, which was heated at 80°C for 24h to synthesize Ag nanoparticles in situ.
25439895	4	49	used	used	684:687	arg2	state					673:677	wet state	669:677	wet state	669:677	In our findings, the pores of the cellulose fiber at wet state were used as a microreactor to synthesize Ag nanopariticles.
25439895	4	49	used	used	684:687	arg2	pores					637:641	the pores	633:641	the pores of the cellulose fiber at wet state	633:677	In our findings, the pores of the cellulose fiber at wet state were used as a microreactor to synthesize Ag nanopariticles.
25439895	4	49	used	used	684:687	arg2	microreactor					694:705	a microreactor	692:705	a microreactor	692:705	In our findings, the pores of the cellulose fiber at wet state were used as a microreactor to synthesize Ag nanopariticles.
25439895	6	50	theme	antibacterial	882:894	arg1	activity					896:903	excellent antibacterial activity	872:903	excellent antibacterial activity of the cellulose nanocomposite fibers against Staphylococcus aureus	872:971	Antibacterial experiment revealed excellent antibacterial activity of the cellulose nanocomposite fibers against Staphylococcus aureus.
26729531	3	0	theme	XR	723:724	arg1	strengths					734:742	all extended-release metformin hydrochloride (Met XR) tablet strengths	673:742	all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries	673:772	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	7	1	theme	combined	1556:1563	arg1	results					1574:1580	the combined modeling results	1552:1580	the combined modeling results	1552:1580	A clinical bioequivalence study was pursued based on the combined modeling results and demonstrated equivalent exposure as predicted by the simulations.
26729531	2	2	theme	release	615:621	arg1	kinetics					623:630	the desired equivalent metformin release kinetics	582:630	the desired equivalent metformin release kinetics	582:630	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	7	3	theme	bioequivalence	1510:1523	arg1	study					1525:1529	A clinical bioequivalence study	1499:1529	A clinical bioequivalence study	1499:1529	A clinical bioequivalence study was pursued based on the combined modeling results and demonstrated equivalent exposure as predicted by the simulations.
26729531	1	4	theme	computational	115:127	arg1	model					149:153	A computational fluid dynamic (CFD) model	113:153	A computational fluid dynamic (CFD) model	113:153	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	3	5	theme	different	753:761	arg1	geometries					763:772	different geometries	753:772	different geometries	753:772	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	1	6	theme	physical	316:323	arg1	properties					338:347	the physical and chemical properties	312:347	the physical and chemical properties of the drug substance	312:369	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	6	theme	physical	316:323	arg1	size					396:399	size	396:399	size	396:399	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	6	theme	physical	316:323	arg1	composition					372:382	composition	372:382	composition	372:382	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	4	7	theme	simulated	917:925	arg1	kinetics					935:942	the simulated release kinetics	913:942	the simulated release kinetics	913:942	Experimental dissolution profiles of three lots of high-strength tablets agreed with the simulated release kinetics.
26729531	1	8	theme	hydroxypropylmethylcellulose	205:232	arg1	formulation					271:281	a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation	203:281	a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet	203:423	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	9	theme	HPMC	235:238	arg1	formulation					271:281	a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation	203:281	a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet	203:423	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	6	10	theme	absorption	1319:1328	arg1	model					1330:1334	the absorption model	1315:1334	the absorption model	1315:1334	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	1	11	theme	tablet	418:423	arg1	properties					338:347	the physical and chemical properties	312:347	the physical and chemical properties of the drug substance	312:369	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	11	theme	tablet	418:423	arg1	size					396:399	size	396:399	size	396:399	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	11	theme	tablet	418:423	arg1	composition					372:382	composition	372:382	composition	372:382	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	11	theme	tablet	418:423	arg1	shape					405:409	shape	405:409	shape	405:409	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	12	theme	matrix-based	241:252	arg1	formulation					271:281	a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation	203:281	a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet	203:423	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	2	13	theme	equivalent	594:603	arg1	kinetics					623:630	the desired equivalent metformin release kinetics	582:630	the desired equivalent metformin release kinetics	582:630	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	3	14	contain	had	749:751	arg2	geometries					763:772	different geometries	753:772	different geometries	753:772	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	3	14	contain	had	749:751	arg1	strengths					734:742	all extended-release metformin hydrochloride (Met XR) tablet strengths	673:742	all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries	673:772	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	3	15	theme	dissolution	806:816	arg1	behavior					818:825	dissolution behavior	806:825	dissolution behavior	806:825	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	6	16	theme	XR	1387:1388	arg1	tablets					1390:1396	new 1000-mg Met XR tablets	1371:1396	new 1000-mg Met XR tablets	1371:1396	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	6	17	theme	1000-mg	1375:1381	arg1	tablets					1390:1396	new 1000-mg Met XR tablets	1371:1396	new 1000-mg Met XR tablets	1371:1396	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	5	18	theme	clinical	1143:1150	arg1	exposure					1152:1159	the clinical exposure	1139:1159	the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study	1139:1259	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	3	19	theme	SA/V	655:658	arg1	ratio					660:664	a similar SA/V ratio	645:664	a similar SA/V ratio	645:664	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	2	20	theme	area/volume	509:519	arg1	approach					528:535	the surface area/volume (SA/V) approach	497:535	the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics	497:630	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	6	21	theme	release	1281:1287	arg1	kinetics					1289:1296	In vitro metformin release kinetics	1262:1296	In vitro metformin release kinetics	1262:1296	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	0	22	theme	Tablet	93:98	arg1	Formulation					100:110	Extended-release Metformin Hydrochloride Tablet Formulation	52:110	Extended-release Metformin Hydrochloride Tablet Formulation	52:110	Mathematical Model-Based Accelerated Development of Extended-release Metformin Hydrochloride Tablet Formulation.
26729531	5	23	theme	tablet	1196:1201	arg1	exposure					1152:1159	the clinical exposure	1139:1159	the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study	1139:1259	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	1	24	from	formulation	271:281	arg1	release					190:196	metformin release	180:196	metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet	180:423	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	25	theme	metformin	180:188	arg1	release					190:196	metformin release	180:196	metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet	180:423	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	5	26	theme	strength	1177:1184	arg1	tablet					1196:1201	the new high strength (1000 mg) tablet	1164:1201	the new high strength (1000 mg) tablet	1164:1201	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	2	27	theme	SA/V	522:525	arg1	approach					528:535	the surface area/volume (SA/V) approach	497:535	the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics	497:630	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	4	28	theme	Experimental	828:839	arg1	profiles					853:860	Experimental dissolution profiles	828:860	Experimental dissolution profiles of three lots of high-strength tablets	828:899	Experimental dissolution profiles of three lots of high-strength tablets agreed with the simulated release kinetics.
26729531	5	29	theme	1000 mg	1187:1193	arg1	tablet					1196:1201	the new high strength (1000 mg) tablet	1164:1201	the new high strength (1000 mg) tablet	1164:1201	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	6	30	dep	in	1455:1456	arg1	vivo					1458:1461	vivo	1458:1461	vivo	1458:1461	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	2	31	theme	tablet	459:464	arg1	geometry					466:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	1	32	theme	fluid	129:133	arg1	model					149:153	A computational fluid dynamic (CFD) model	113:153	A computational fluid dynamic (CFD) model	113:153	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	2	33	theme	1000 mg	450:456	arg1	geometry					466:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	2	34	theme	strength	440:447	arg1	geometry					466:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	5	35	theme	known	1039:1043	arg1	properties					1104:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties	1039:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin	1039:1126	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	3	36	theme	extended-release	677:692	arg1	strengths					734:742	all extended-release metformin hydrochloride (Met XR) tablet strengths	673:742	all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries	673:772	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	2	37	theme	high	430:433	arg1	geometry					466:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	4	38	theme	tablets	893:899	arg1	lots					871:874	three lots	865:874	three lots of high-strength tablets	865:899	Experimental dissolution profiles of three lots of high-strength tablets agreed with the simulated release kinetics.
26729531	4	38	theme	tablets	893:899	arg1	tablets					893:899	high-strength tablets	879:899	high-strength tablets	879:899	Experimental dissolution profiles of three lots of high-strength tablets agreed with the simulated release kinetics.
26729531	0	39	theme	Metformin	69:77	arg1	Formulation					100:110	Extended-release Metformin Hydrochloride Tablet Formulation	52:110	Extended-release Metformin Hydrochloride Tablet Formulation	52:110	Mathematical Model-Based Accelerated Development of Extended-release Metformin Hydrochloride Tablet Formulation.
26729531	5	40	theme	clinical	1231:1238	arg1	study					1255:1259	a human clinical bioequivalence study	1223:1259	a human clinical bioequivalence study	1223:1259	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	7	41	theme	equivalent	1599:1608	arg1	exposure					1610:1617	equivalent exposure	1599:1617	equivalent exposure	1599:1617	A clinical bioequivalence study was pursued based on the combined modeling results and demonstrated equivalent exposure as predicted by the simulations.
26729531	3	42	theme	Met	719:721	arg1	strengths					734:742	all extended-release metformin hydrochloride (Met XR) tablet strengths	673:742	all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries	673:772	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	1	43	theme	extended-release	254:269	arg1	formulation					271:281	a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation	203:281	a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet	203:423	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	5	44	theme	in	1083:1084	arg1	properties					1104:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties	1039:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin	1039:1126	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	3	45	theme	hydrochloride	704:716	arg1	strengths					734:742	all extended-release metformin hydrochloride (Met XR) tablet strengths	673:742	all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries	673:772	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	0	46	theme	Hydrochloride	79:91	arg1	Formulation					100:110	Extended-release Metformin Hydrochloride Tablet Formulation	52:110	Extended-release Metformin Hydrochloride Tablet Formulation	52:110	Mathematical Model-Based Accelerated Development of Extended-release Metformin Hydrochloride Tablet Formulation.
26729531	7	47	theme	modeling	1565:1572	arg1	results					1574:1580	the combined modeling results	1552:1580	the combined modeling results	1552:1580	A clinical bioequivalence study was pursued based on the combined modeling results and demonstrated equivalent exposure as predicted by the simulations.
26729531	5	48	theme	dissolution	1092:1102	arg1	properties					1104:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties	1039:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin	1039:1126	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	5	49	theme	human	1225:1229	arg1	study					1255:1259	a human clinical bioequivalence study	1223:1259	a human clinical bioequivalence study	1223:1259	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	6	50	theme	new	1371:1373	arg1	tablets					1390:1396	new 1000-mg Met XR tablets	1371:1396	new 1000-mg Met XR tablets	1371:1396	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	4	51	theme	release	927:933	arg1	kinetics					935:942	the simulated release kinetics	913:942	the simulated release kinetics	913:942	Experimental dissolution profiles of three lots of high-strength tablets agreed with the simulated release kinetics.
26729531	6	52	from	exposures	1347:1355	arg1	humans					1360:1365	humans	1360:1365	humans	1360:1365	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	2	53	theme	metformin	605:613	arg1	kinetics					623:630	the desired equivalent metformin release kinetics	582:630	the desired equivalent metformin release kinetics	582:630	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	6	54	theme	In	1262:1263	arg1	kinetics					1289:1296	In vitro metformin release kinetics	1262:1296	In vitro metformin release kinetics	1262:1296	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	7	55	theme	clinical	1501:1508	arg1	study					1525:1529	A clinical bioequivalence study	1499:1529	A clinical bioequivalence study	1499:1529	A clinical bioequivalence study was pursued based on the combined modeling results and demonstrated equivalent exposure as predicted by the simulations.
26729531	1	56	theme	substance	361:369	arg1	properties					338:347	the physical and chemical properties	312:347	the physical and chemical properties of the drug substance	312:369	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	56	theme	substance	361:369	arg1	size					396:399	size	396:399	size	396:399	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	56	theme	substance	361:369	arg1	composition					372:382	composition	372:382	composition	372:382	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	6	57	theme	absorption	1407:1416	arg1	model					1418:1422	the absorption model	1403:1422	the absorption model	1403:1422	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	5	58	theme	absorption	977:986	arg1	model					988:992	a pharmacokinetic absorption model	959:992	a pharmacokinetic absorption model	959:992	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	3	59	theme	similar	783:789	arg1	simulations					791:801	similar simulations	783:801	similar simulations of dissolution behavior	783:825	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	5	60	dep	in	1083:1084	arg1	vitro					1086:1090	vitro	1086:1090	vitro	1086:1090	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	5	61	theme	high	1172:1175	arg1	tablet					1196:1201	the new high strength (1000 mg) tablet	1164:1201	the new high strength (1000 mg) tablet	1164:1201	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	1	62	theme	CFD	144:146	arg1	model					149:153	A computational fluid dynamic (CFD) model	113:153	A computational fluid dynamic (CFD) model	113:153	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	2	63	theme	desired	586:592	arg1	kinetics					623:630	the desired equivalent metformin release kinetics	582:630	the desired equivalent metformin release kinetics	582:630	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	3	64	theme	behavior	818:825	arg1	simulations					791:801	similar simulations	783:801	similar simulations of dissolution behavior	783:825	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	6	65	theme	Met	1383:1385	arg1	tablets					1390:1396	new 1000-mg Met XR tablets	1371:1396	new 1000-mg Met XR tablets	1371:1396	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	3	66	theme	similar	647:653	arg1	ratio					660:664	a similar SA/V ratio	645:664	a similar SA/V ratio	645:664	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	0	67	theme	Formulation	100:110	arg1	Development					37:47	Development	37:47	Development of Extended-release Metformin Hydrochloride Tablet Formulation	37:110	Mathematical Model-Based Accelerated Development of Extended-release Metformin Hydrochloride Tablet Formulation.
26729531	2	68	theme	surface	501:507	arg1	approach					528:535	the surface area/volume (SA/V) approach	497:535	the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics	497:630	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	6	69	theme	metformin	1271:1279	arg1	kinetics					1289:1296	In vitro metformin release kinetics	1262:1296	In vitro metformin release kinetics	1262:1296	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	5	70	theme	pharmacokinetic	961:975	arg1	model					988:992	a pharmacokinetic absorption model	959:992	a pharmacokinetic absorption model	959:992	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	6	71	theme	equivalent	1444:1453	arg1	exposure					1463:1470	equivalent in vivo exposure	1444:1470	equivalent in vivo exposure	1444:1470	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	6	72	dep	equivalent	1444:1453	arg1	in					1455:1456	in	1455:1456	in	1455:1456	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	5	73	theme	physicochemical	1045:1059	arg1	properties					1104:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties	1039:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin	1039:1126	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	5	74	theme	pharmacokinetic	1062:1076	arg1	properties					1104:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties	1039:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin	1039:1126	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	5	75	theme	GastroPlus™	1014:1024	arg1	software					1026:1033	GastroPlus™ software	1014:1033	GastroPlus™ software	1014:1033	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	1	76	theme	chemical	329:336	arg1	properties					338:347	the physical and chemical properties	312:347	the physical and chemical properties of the drug substance	312:369	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	76	theme	chemical	329:336	arg1	size					396:399	size	396:399	size	396:399	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	76	theme	chemical	329:336	arg1	composition					372:382	composition	372:382	composition	372:382	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	1	77	theme	dynamic	135:141	arg1	model					149:153	A computational fluid dynamic (CFD) model	113:153	A computational fluid dynamic (CFD) model	113:153	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	2	78	theme	dose	435:438	arg1	geometry					466:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	0	79	theme	Extended-release	52:67	arg1	Formulation					100:110	Extended-release Metformin Hydrochloride Tablet Formulation	52:110	Extended-release Metformin Hydrochloride Tablet Formulation	52:110	Mathematical Model-Based Accelerated Development of Extended-release Metformin Hydrochloride Tablet Formulation.
26729531	4	80	theme	lots	871:874	arg1	profiles					853:860	Experimental dissolution profiles	828:860	Experimental dissolution profiles of three lots of high-strength tablets	828:899	Experimental dissolution profiles of three lots of high-strength tablets agreed with the simulated release kinetics.
26729531	2	81	theme	New	426:428	arg1	geometry					466:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry	426:473	New high dose strength (1000 mg) tablet geometry was selected based on the surface area/volume (SA/V) approach advocated by Lapidus/Lordi/Reynold to obtain the desired equivalent metformin release kinetics.
26729531	6	82	theme	dose	1483:1486	arg1	strengths					1488:1496	all dose strengths	1479:1496	all dose strengths	1479:1496	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	1	83	theme	drug	356:359	arg1	substance					361:369	the drug substance	352:369	the drug substance	352:369	A computational fluid dynamic (CFD) model was developed to predict metformin release from a hydroxypropylmethylcellulose (HPMC) matrix-based extended-release formulation that took into consideration the physical and chemical properties of the drug substance, composition, as well as size and shape of the tablet.
26729531	5	84	theme	metformin	1118:1126	arg1	software					1026:1033	GastroPlus™ software	1014:1033	GastroPlus™ software	1014:1033	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	5	84	theme	metformin	1118:1126	arg1	properties					1104:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties	1039:1113	known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin	1039:1126	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	4	85	theme	high-strength	879:891	arg1	tablets					893:899	high-strength tablets	879:899	high-strength tablets	879:899	Experimental dissolution profiles of three lots of high-strength tablets agreed with the simulated release kinetics.
26729531	5	86	theme	new	1168:1170	arg1	tablet					1196:1201	the new high strength (1000 mg) tablet	1164:1201	the new high strength (1000 mg) tablet	1164:1201	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	5	87	theme	bioequivalence	1240:1253	arg1	study					1255:1259	a human clinical bioequivalence study	1223:1259	a human clinical bioequivalence study	1223:1259	Additionally, a pharmacokinetic absorption model was developed using GastroPlus™ software and known physicochemical, pharmacokinetic, and in vitro dissolution properties of metformin to predict the clinical exposure of the new high strength (1000 mg) tablet prior to conducting a human clinical bioequivalence study.
26729531	3	88	theme	metformin	694:702	arg1	strengths					734:742	all extended-release metformin hydrochloride (Met XR) tablet strengths	673:742	all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries	673:772	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	4	89	theme	dissolution	841:851	arg1	profiles					853:860	Experimental dissolution profiles	828:860	Experimental dissolution profiles of three lots of high-strength tablets	828:899	Experimental dissolution profiles of three lots of high-strength tablets agreed with the simulated release kinetics.
26729531	6	90	dep	In	1262:1263	arg1	vitro					1265:1269	vitro	1265:1269	vitro	1265:1269	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26729531	3	91	theme	tablet	727:732	arg1	strengths					734:742	all extended-release metformin hydrochloride (Met XR) tablet strengths	673:742	all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries	673:772	Maintaining a similar SA/V ratio across all extended-release metformin hydrochloride (Met XR) tablet strengths that had different geometries provided similar simulations of dissolution behavior.
26729531	6	92	used	utilized	1303:1310	arg2	kinetics					1289:1296	In vitro metformin release kinetics	1262:1296	In vitro metformin release kinetics	1262:1296	In vitro metformin release kinetics were utilized in the absorption model to predict exposures in humans for new 1000-mg Met XR tablets, and the absorption model correctly projected equivalent in vivo exposure across all dose strengths.
26481909	7	0	dep	studies	1482:1488	arg1	the					1447:1449	the	1447:1449	the	1447:1449	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	7	0	dep	studies	1482:1488	arg1	basis					1451:1455	basis	1451:1455	basis	1451:1455	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	6	1	theme	colloidal	983:991	arg1	network					993:999	a colloidal network	981:999	a colloidal network dominated by hydrophobic interactions at high temperature	981:1057	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	4	2	theme	large	506:510	arg1	tests					556:560	both the small and large amplitude oscillatory shear (SAOS and LAOS) tests	487:560	tests	556:560	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	0	3	theme	hydrogel	76:83	arg1	nature					19:24	the nature	15:24	the nature of a unique natural polymer-based thermosensitive hydrogel	15:83	Exploration of the nature of a unique natural polymer-based thermosensitive hydrogel.
26481909	5	4	theme	gelation	789:796	arg1	mechanism					798:806	the possible gelation mechanism	776:806	the possible gelation mechanism of this unique thermosensitive hydrogel	776:846	The purpose of such tests is to investigate the structural change of the hydrogel network and thus to understand the possible gelation mechanism of this unique thermosensitive hydrogel.
26481909	2	5	theme	most	260:263	arg1	systems					289:295	most thermosensitive gelling systems	260:295	most thermosensitive gelling systems	260:295	Unlike most thermosensitive gelling systems, the CS/GP system is only partially reversible.
26481909	6	6	theme	high	1042:1045	arg1	temperature					1047:1057	high temperature	1042:1057	high temperature	1042:1057	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	1	7	theme	injectable	221:230	arg1	material					243:250	an injectable biomedical material	218:250	an injectable biomedical material	218:250	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	4	8	dep	small	496:500	arg1	shear					534:538	amplitude oscillatory shear	512:538	amplitude oscillatory shear (SAOS and LAOS)	512:554	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	7	9	from	studies	1482:1488	arg1	laboratory					1514:1523	this laboratory	1509:1523	this laboratory	1509:1523	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	6	10	with	results	863:869	arg1	method					925:930	the Chebyshev expansion method	901:930	the Chebyshev expansion method	901:930	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	1	11	theme	biomedical	232:241	arg1	material					243:250	an injectable biomedical material	218:250	an injectable biomedical material	218:250	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	7	12	theme	practical	1326:1334	arg1	vision					1366:1371	a clearer vision	1356:1371	a clearer vision	1356:1371	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	7	12	theme	practical	1326:1334	arg1	example					1336:1342	a practical example	1324:1342	a practical example	1324:1342	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	7	13	theme	other	1529:1533	arg1	groups					1544:1549	other research groups	1529:1549	other research groups	1529:1549	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	4	14	theme	CS/GP	583:587	arg1	samples					598:604	the fully matured CS/GP hydrogel samples	565:604	the fully matured CS/GP hydrogel samples	565:604	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	4	15	theme	amplitude	512:520	arg1	shear					534:538	amplitude oscillatory shear	512:538	amplitude oscillatory shear (SAOS and LAOS)	512:554	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	6	16	theme	Chebyshev	905:913	arg1	method					925:930	the Chebyshev expansion method	901:930	the Chebyshev expansion method	901:930	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	7	17	theme	CS/GP	1413:1417	arg1	hydrogel					1435:1442	the unique CS/GP thermosensitive hydrogel	1402:1442	the unique CS/GP thermosensitive hydrogel	1402:1442	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	1	18	theme	heat	142:145	arg1	system					130:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	1	18	theme	heat	142:145	arg1	system					163:168	a heat induced gelling system	140:168	a heat induced gelling system with a promising potential application, such as an injectable biomedical material	140:250	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	5	19	theme	such	678:681	arg1	tests					683:687	such tests	678:687	such tests	678:687	The purpose of such tests is to investigate the structural change of the hydrogel network and thus to understand the possible gelation mechanism of this unique thermosensitive hydrogel.
26481909	4	20	theme	cooling	646:652	arg1	process					654:660	the cooling process	642:660	the cooling process	642:660	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	1	21	theme	induced	147:153	arg1	system					130:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	1	21	theme	induced	147:153	arg1	system					163:168	a heat induced gelling system	140:168	a heat induced gelling system with a promising potential application, such as an injectable biomedical material	140:250	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	5	22	theme	structural	711:720	arg1	change					722:727	the structural change	707:727	the structural change of the hydrogel network	707:751	The purpose of such tests is to investigate the structural change of the hydrogel network and thus to understand the possible gelation mechanism of this unique thermosensitive hydrogel.
26481909	6	23	theme	further	879:885	arg1	analysis					887:894	the further analysis	875:894	the further analysis with the Chebyshev expansion method	875:930	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	7	24	theme	clearer	1358:1364	arg1	vision					1366:1371	a clearer vision	1356:1371	a clearer vision	1356:1371	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	7	24	theme	clearer	1358:1364	arg1	example					1336:1342	a practical example	1324:1342	a practical example	1324:1342	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	1	25	theme	chitosan	90:97	arg1	system					130:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	1	25	theme	chitosan	90:97	arg1	system					163:168	a heat induced gelling system	140:168	a heat induced gelling system with a promising potential application, such as an injectable biomedical material	140:250	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	6	26	from	temperature	1047:1057	arg1	interactions					1026:1037	hydrophobic interactions	1014:1037	hydrophobic interactions at high temperature	1014:1057	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	1	27	theme	gelling	155:161	arg1	system					130:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	1	27	theme	gelling	155:161	arg1	system					163:168	a heat induced gelling system	140:168	a heat induced gelling system with a promising potential application, such as an injectable biomedical material	140:250	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	0	28	theme	nature	19:24	arg1	Exploration					0:10	Exploration	0:10	Exploration of the nature of a unique natural polymer-based thermosensitive hydrogel	0:83	Exploration of the nature of a unique natural polymer-based thermosensitive hydrogel.
26481909	7	29	theme	research	1535:1542	arg1	groups					1544:1549	other research groups	1529:1549	other research groups	1529:1549	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	5	30	theme	hydrogel	839:846	arg1	mechanism					798:806	the possible gelation mechanism	776:806	the possible gelation mechanism of this unique thermosensitive hydrogel	776:846	The purpose of such tests is to investigate the structural change of the hydrogel network and thus to understand the possible gelation mechanism of this unique thermosensitive hydrogel.
26481909	2	31	theme	gelling	281:287	arg1	systems					289:295	most thermosensitive gelling systems	260:295	most thermosensitive gelling systems	260:295	Unlike most thermosensitive gelling systems, the CS/GP system is only partially reversible.
26481909	4	32	from	tests	556:560	arg1	samples					598:604	the fully matured CS/GP hydrogel samples	565:604	the fully matured CS/GP hydrogel samples	565:604	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	6	33	theme	CS/GP	951:955	arg1	hydrogel					957:964	the CS/GP hydrogel	947:964	the CS/GP hydrogel	947:964	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	5	34	theme	unique	816:821	arg1	hydrogel					839:846	this unique thermosensitive hydrogel	811:846	this unique thermosensitive hydrogel	811:846	The purpose of such tests is to investigate the structural change of the hydrogel network and thus to understand the possible gelation mechanism of this unique thermosensitive hydrogel.
26481909	4	35	theme	small	496:500	arg1	tests					556:560	both the small and large amplitude oscillatory shear (SAOS and LAOS) tests	487:560	tests	556:560	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	2	36	theme	thermosensitive	265:279	arg1	systems					289:295	most thermosensitive gelling systems	260:295	most thermosensitive gelling systems	260:295	Unlike most thermosensitive gelling systems, the CS/GP system is only partially reversible.
26481909	5	37	theme	hydrogel	736:743	arg1	network					745:751	the hydrogel network	732:751	the hydrogel network	732:751	The purpose of such tests is to investigate the structural change of the hydrogel network and thus to understand the possible gelation mechanism of this unique thermosensitive hydrogel.
26481909	5	38	theme	tests	683:687	arg1	purpose					667:673	The purpose	663:673	The purpose of such tests	663:687	The purpose of such tests is to investigate the structural change of the hydrogel network and thus to understand the possible gelation mechanism of this unique thermosensitive hydrogel.
26481909	3	39	theme	liquid	439:444	arg1	state					446:450	its initial liquid state	427:450	its initial liquid state	427:450	That is once the hydrogel is fully matured, it only softens but cannot go back to its initial liquid state when cooled down.
26481909	1	40	with	system	163:168	arg1	application					197:207	a promising potential application	175:207	a promising potential application	175:207	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	7	41	dep	conclude	1184:1191	arg1	may					1180:1182	may	1180:1182	may	1180:1182	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	7	42	theme	polymer	1260:1266	arg1	related					1291:1297	related	1291:1297	related	1291:1297	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	7	42	theme	polymer	1260:1266	arg1	system					1268:1273	a polymer system	1258:1273	a polymer system that is closely related to its structure	1258:1314	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	4	43	theme	matured	575:581	arg1	samples					598:604	the fully matured CS/GP hydrogel samples	565:604	the fully matured CS/GP hydrogel samples	565:604	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	5	44	theme	network	745:751	arg1	change					722:727	the structural change	707:727	the structural change of the hydrogel network	707:751	The purpose of such tests is to investigate the structural change of the hydrogel network and thus to understand the possible gelation mechanism of this unique thermosensitive hydrogel.
26481909	6	45	theme	LAOS	858:861	arg1	results					863:869	the LAOS results	854:869	the LAOS results	854:869	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	7	46	theme	thermosensitive	1419:1433	arg1	hydrogel					1435:1442	the unique CS/GP thermosensitive hydrogel	1402:1442	the unique CS/GP thermosensitive hydrogel	1402:1442	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	4	47	dep	tests	556:560	arg1	both					487:490	both	487:490	both	487:490	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	1	48	theme	promising	177:185	arg1	application					197:207	a promising potential application	175:207	a promising potential application	175:207	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	0	49	theme	natural	38:44	arg1	hydrogel					76:83	a unique natural polymer-based thermosensitive hydrogel	29:83	a unique natural polymer-based thermosensitive hydrogel	29:83	Exploration of the nature of a unique natural polymer-based thermosensitive hydrogel.
26481909	5	50	theme	possible	780:787	arg1	mechanism					798:806	the possible gelation mechanism	776:806	the possible gelation mechanism of this unique thermosensitive hydrogel	776:846	The purpose of such tests is to investigate the structural change of the hydrogel network and thus to understand the possible gelation mechanism of this unique thermosensitive hydrogel.
26481909	7	51	theme	gelation	1380:1387	arg1	mechanism					1389:1397	the gelation mechanism	1376:1397	the gelation mechanism of the unique CS/GP thermosensitive hydrogel	1376:1442	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	7	52	from	assumptions	1494:1504	arg1	laboratory					1514:1523	this laboratory	1509:1523	this laboratory	1509:1523	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	6	53	theme	hydrophobic	1014:1024	arg1	interactions					1026:1037	hydrophobic interactions	1014:1037	hydrophobic interactions at high temperature	1014:1057	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	3	54	theme	initial	431:437	arg1	state					446:450	its initial liquid state	427:450	its initial liquid state	427:450	That is once the hydrogel is fully matured, it only softens but cannot go back to its initial liquid state when cooled down.
26481909	0	55	theme	unique	31:36	arg1	hydrogel					76:83	a unique natural polymer-based thermosensitive hydrogel	29:83	a unique natural polymer-based thermosensitive hydrogel	29:83	Exploration of the nature of a unique natural polymer-based thermosensitive hydrogel.
26481909	7	56	theme	powerful	1208:1215	arg1	LOAS					1198:1201	LOAS	1198:1201	LOAS	1198:1201	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	7	56	theme	powerful	1208:1215	arg1	tool					1217:1220	a powerful tool	1206:1220	a powerful tool	1206:1220	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	4	57	theme	oscillatory	522:532	arg1	shear					534:538	amplitude oscillatory shear	512:538	amplitude oscillatory shear (SAOS and LAOS)	512:554	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	6	58	theme	hydrogen	1117:1124	arg1	bonding					1126:1132	hydrogen bonding	1117:1132	hydrogen bonding	1117:1132	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	1	59	theme	/β-glycerol	103:113	arg1	system					130:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	1	59	theme	/β-glycerol	103:113	arg1	system					163:168	a heat induced gelling system	140:168	a heat induced gelling system with a promising potential application, such as an injectable biomedical material	140:250	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	7	60	theme	considerable	1460:1471	arg1	studies					1482:1488	considerable previous studies	1460:1488	considerable previous studies	1460:1488	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	7	61	from	groups	1544:1549	arg1	laboratory					1514:1523	this laboratory	1509:1523	this laboratory	1509:1523	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	7	62	theme	nonlinear	1235:1243	arg1	behaviour					1245:1253	the nonlinear behaviour	1231:1253	the nonlinear behaviour of a polymer system that is closely related to its structure	1231:1314	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	7	63	theme	system	1268:1273	arg1	behaviour					1245:1253	the nonlinear behaviour	1231:1253	the nonlinear behaviour of a polymer system that is closely related to its structure	1231:1314	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	1	64	theme	phosphate	115:123	arg1	system					130:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	1	64	theme	phosphate	115:123	arg1	system					163:168	a heat induced gelling system	140:168	a heat induced gelling system with a promising potential application, such as an injectable biomedical material	140:250	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	1	65	theme	potential	187:195	arg1	application					197:207	a promising potential application	175:207	a promising potential application	175:207	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	5	66	theme	thermosensitive	823:837	arg1	hydrogel					839:846	this unique thermosensitive hydrogel	811:846	this unique thermosensitive hydrogel	811:846	The purpose of such tests is to investigate the structural change of the hydrogel network and thus to understand the possible gelation mechanism of this unique thermosensitive hydrogel.
26481909	6	67	with	analysis	887:894	arg1	method					925:930	the Chebyshev expansion method	901:930	the Chebyshev expansion method	901:930	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	4	68	theme	temperatures	622:633	arg1	temperatures					622:633	temperatures	622:633	temperatures within the cooling process	622:660	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	4	68	theme	temperatures	622:633	arg1	variety					611:617	a variety	609:617	a variety of temperatures within the cooling process	609:660	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	2	69	theme	CS/GP	302:306	arg1	system					308:313	the CS/GP system	298:313	the CS/GP system	298:313	Unlike most thermosensitive gelling systems, the CS/GP system is only partially reversible.
26481909	2	69	theme	CS/GP	302:306	arg1	reversible					333:342	reversible	333:342	reversible	333:342	Unlike most thermosensitive gelling systems, the CS/GP system is only partially reversible.
26481909	0	70	theme	thermosensitive	60:74	arg1	hydrogel					76:83	a unique natural polymer-based thermosensitive hydrogel	29:83	a unique natural polymer-based thermosensitive hydrogel	29:83	Exploration of the nature of a unique natural polymer-based thermosensitive hydrogel.
26481909	4	71	dep	shear	534:538	arg1	LAOS					550:553	LAOS	550:553	LAOS	550:553	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	4	71	dep	shear	534:538	arg1	SAOS					541:544	SAOS	541:544	SAOS	541:544	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	7	72	theme	unique	1406:1411	arg1	hydrogel					1435:1442	the unique CS/GP thermosensitive hydrogel	1402:1442	the unique CS/GP thermosensitive hydrogel	1402:1442	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	1	73	theme	GP	126:127	arg1	system					130:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system	86:135	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	1	73	theme	GP	126:127	arg1	system					163:168	a heat induced gelling system	140:168	a heat induced gelling system with a promising potential application, such as an injectable biomedical material	140:250	The chitosan (CS)/β-glycerol phosphate (GP) system is a heat induced gelling system with a promising potential application, such as an injectable biomedical material.
26481909	7	74	theme	hydrogel	1435:1442	arg1	mechanism					1389:1397	the gelation mechanism	1376:1397	the gelation mechanism of the unique CS/GP thermosensitive hydrogel	1376:1442	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	0	75	theme	polymer-based	46:58	arg1	hydrogel					76:83	a unique natural polymer-based thermosensitive hydrogel	29:83	a unique natural polymer-based thermosensitive hydrogel	29:83	Exploration of the nature of a unique natural polymer-based thermosensitive hydrogel.
26481909	6	76	theme	flexible	1087:1094	arg1	network					1096:1102	a flexible network	1085:1102	a flexible network dominated by hydrogen bonding when the temperature goes down	1085:1163	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
26481909	4	77	theme	hydrogel	589:596	arg1	samples					598:604	the fully matured CS/GP hydrogel samples	565:604	the fully matured CS/GP hydrogel samples	565:604	Here, we perform both the small and large amplitude oscillatory shear (SAOS and LAOS) tests on the fully matured CS/GP hydrogel samples at a variety of temperatures within the cooling process.
26481909	7	78	theme	previous	1473:1480	arg1	studies					1482:1488	considerable previous studies	1460:1488	considerable previous studies	1460:1488	Therefore, we may conclude that LOAS is a powerful tool to study the nonlinear behaviour of a polymer system that is closely related to its structure, and as a practical example, we achieve a clearer vision on the gelation mechanism of the unique CS/GP thermosensitive hydrogel on the basis of considerable previous studies and assumptions in this laboratory and other research groups.
26481909	6	79	theme	expansion	915:923	arg1	method					925:930	the Chebyshev expansion method	901:930	the Chebyshev expansion method	901:930	From the LAOS results and the further analysis with the Chebyshev expansion method, it shows that the CS/GP hydrogel is composed of a colloidal network dominated by hydrophobic interactions at high temperature, and gradually turns into a flexible network dominated by hydrogen bonding when the temperature goes down.
28034633	0	0	theme	brush	63:67	arg1	border					69:74	the intestinal brush border	48:74	the intestinal brush border	48:74	Glycol chitosan: A stabilizer of lipid rafts in the intestinal brush border.
28034633	2	1	theme	model	431:435	arg1	bilayers					437:444	model bilayers	431:444	model bilayers	431:444	Recently, glycol chitosan (GC), a soluble derivative of chitosan, was shown to bind specifically to lipid raft domains in model bilayers.
28034633	5	2	theme	subunit	1032:1038	arg1	presence					1004:1011	the simultaneous presence	987:1011	the simultaneous presence of cholera toxin B subunit (CTB)	987:1044	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	6	3	theme	resistant	1160:1168	arg1	DRMs					1181:1184	DRMs	1181:1184	DRMs	1181:1184	Biochemically, GC bound to microvillus membrane vesicles and caused a change in the density profile of detergent resistant membranes (DRMs).
28034633	6	3	theme	resistant	1160:1168	arg1	membranes					1170:1178	detergent resistant membranes	1150:1178	detergent resistant membranes (DRMs)	1150:1185	Biochemically, GC bound to microvillus membrane vesicles and caused a change in the density profile of detergent resistant membranes (DRMs).
28034633	0	4	theme	intestinal	52:61	arg1	border					69:74	the intestinal brush border	48:74	the intestinal brush border	48:74	Glycol chitosan: A stabilizer of lipid rafts in the intestinal brush border.
28034633	4	5	theme	model	782:786	arg1	system					788:793	a model system	780:793	a model system	780:793	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	4	5	theme	model	782:786	arg1	culture					737:743	organ culture	731:743	organ culture of pig jejunal mucosal explants	731:775	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	3	6	theme	small	451:455	arg1	membrane					481:488	The small intestinal brush border membrane	447:488	The small intestinal brush border membrane	447:488	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	6	7	from	change	1117:1122	arg1	profile					1139:1145	the density profile	1127:1145	the density profile of detergent resistant membranes (DRMs)	1127:1185	Biochemically, GC bound to microvillus membrane vesicles and caused a change in the density profile of detergent resistant membranes (DRMs).
28034633	8	8	dep	stabilizing	1387:1397	arg1	detergent-protective					1400:1419	detergent-protective	1400:1419	detergent-protective	1400:1419	Instead, and unlike CTB, FITC-GC seems to exert a stabilizing, detergent-protective effect on the lipid raft organization of the brush border.
28034633	5	9	theme	little	872:877	arg1	uptake					879:884	Only little uptake	867:884	Only little uptake via constitutive endocytosis into apical early endosomes	867:941	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	1	10	theme	chitosan	230:237	arg1	candidates					253:262	potential candidates	243:262	potential candidates as enhancers for transmucosal drug delivery	243:306	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	1	10	theme	chitosan	230:237	arg1	derivatives					215:225	chemical derivatives	206:225	chemical derivatives of chitosan	206:237	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	4	11	theme	brush	853:857	arg1	border					859:864	the brush border	849:864	the brush border	849:864	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	5	12	theme	cholera	1016:1022	arg1	CTB					1041:1043	CTB	1041:1043	CTB	1041:1043	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	5	12	theme	cholera	1016:1022	arg1	subunit					1032:1038	cholera toxin B subunit	1016:1038	cholera toxin B subunit (CTB)	1016:1044	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	6	13	theme	membrane	1086:1093	arg1	vesicles					1095:1102	microvillus membrane vesicles	1074:1102	microvillus membrane vesicles	1074:1102	Biochemically, GC bound to microvillus membrane vesicles and caused a change in the density profile of detergent resistant membranes (DRMs).
28034633	3	14	theme	FITC-conjugated	658:672	arg1	FITC-GC					678:684	FITC-GC	678:684	FITC-GC	678:684	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	3	14	theme	FITC-conjugated	658:672	arg1	GC					674:675	FITC-conjugated GC	658:675	FITC-conjugated GC (FITC-GC)	658:685	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	4	15	theme	explants	768:775	arg1	system					788:793	a model system	780:793	a model system	780:793	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	4	15	theme	explants	768:775	arg1	culture					737:743	organ culture	731:743	organ culture of pig jejunal mucosal explants	731:775	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	3	16	theme	border	474:479	arg1	membrane					481:488	The small intestinal brush border membrane	447:488	The small intestinal brush border membrane	447:488	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	4	17	theme	mucosal	760:766	arg1	explants					768:775	pig jejunal mucosal explants	748:775	pig jejunal mucosal explants	748:775	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	2	18	from	domains	420:426	arg1	bilayers					437:444	model bilayers	431:444	model bilayers	431:444	Recently, glycol chitosan (GC), a soluble derivative of chitosan, was shown to bind specifically to lipid raft domains in model bilayers.
28034633	4	19	theme	organ	731:735	arg1	system					788:793	a model system	780:793	a model system	780:793	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	4	19	theme	organ	731:735	arg1	culture					737:743	organ culture	731:743	organ culture of pig jejunal mucosal explants	731:775	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	5	20	theme	constitutive	890:901	arg1	endocytosis					903:913	constitutive endocytosis	890:913	constitutive endocytosis	890:913	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	1	21	theme	-linked	140:146	arg1	units					160:164	β-(1-4)-linked glucosamine units	133:164	β-(1-4)-linked glucosamine units	133:164	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	0	22	from	stabilizer	19:28	arg1	border					69:74	the intestinal brush border	48:74	the intestinal brush border	48:74	Glycol chitosan: A stabilizer of lipid rafts in the intestinal brush border.
28034633	6	23	theme	detergent	1150:1158	arg1	DRMs					1181:1184	DRMs	1181:1184	DRMs	1181:1184	Biochemically, GC bound to microvillus membrane vesicles and caused a change in the density profile of detergent resistant membranes (DRMs).
28034633	6	23	theme	detergent	1150:1158	arg1	membranes					1170:1178	detergent resistant membranes	1150:1178	detergent resistant membranes (DRMs)	1150:1185	Biochemically, GC bound to microvillus membrane vesicles and caused a change in the density profile of detergent resistant membranes (DRMs).
28034633	5	24	theme	apical	920:925	arg1	endosomes					933:941	apical early endosomes	920:941	apical early endosomes	920:941	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	3	25	theme	raft	509:512	arg1	composition					514:524	a unique lipid raft composition	494:524	a unique lipid raft composition	494:524	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	5	26	theme	toxin	1024:1028	arg1	CTB					1041:1043	CTB	1041:1043	CTB	1041:1043	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	5	26	theme	toxin	1024:1028	arg1	subunit					1032:1038	cholera toxin B subunit	1016:1038	cholera toxin B subunit (CTB)	1016:1044	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	1	27	theme	glucosamine	148:158	arg1	units					160:164	β-(1-4)-linked glucosamine units	133:164	β-(1-4)-linked glucosamine units	133:164	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	0	28	theme	Glycol	0:5	arg1	chitosan					7:14	Glycol chitosan	0:14	Glycol chitosan: A stabilizer of lipid rafts in the intestinal brush border.	0:75	Glycol chitosan: A stabilizer of lipid rafts in the intestinal brush border.
28034633	1	29	theme	potential	243:251	arg1	candidates					253:262	potential candidates	243:262	potential candidates as enhancers for transmucosal drug delivery	243:306	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	1	29	theme	potential	243:251	arg1	derivatives					215:225	chemical derivatives	206:225	chemical derivatives of chitosan	206:237	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	1	30	link	-linked	140:146	arg1	units					160:164	β-(1-4)-linked glucosamine units	133:164	β-(1-4)-linked glucosamine units	133:164	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	6	31	theme	membranes	1170:1178	arg1	profile					1139:1145	the density profile	1127:1145	the density profile of detergent resistant membranes (DRMs)	1127:1185	Biochemically, GC bound to microvillus membrane vesicles and caused a change in the density profile of detergent resistant membranes (DRMs).
28034633	3	32	theme	intestinal	702:711	arg1	epithelium					713:722	the small intestinal epithelium	692:722	the small intestinal epithelium	692:722	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	3	33	theme	glycolipids	547:557	arg1	amounts					536:542	high amounts	531:542	high amounts of glycolipids cross-linked by lectins	531:581	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	3	33	theme	glycolipids	547:557	arg1	glycolipids					547:557	glycolipids	547:557	glycolipids cross-linked by lectins	547:581	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	4	34	theme	FITC-GC	838:844	arg1	binding					819:825	widespread binding	808:825	widespread binding of luminal FITC-GC to the brush border	808:864	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	3	35	theme	unique	496:501	arg1	composition					514:524	a unique lipid raft composition	494:524	a unique lipid raft composition	494:524	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	3	36	theme	GC	674:675	arg1	interaction					643:653	the interaction	639:653	the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium	639:722	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	4	37	theme	luminal	830:836	arg1	FITC-GC					838:844	luminal FITC-GC	830:844	luminal FITC-GC	830:844	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	3	38	theme	lipid	503:507	arg1	composition					514:524	a unique lipid raft composition	494:524	a unique lipid raft composition	494:524	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	8	39	theme	raft	1441:1444	arg1	organization					1446:1457	the lipid raft organization	1431:1457	the lipid raft organization of the brush border	1431:1477	Instead, and unlike CTB, FITC-GC seems to exert a stabilizing, detergent-protective effect on the lipid raft organization of the brush border.
28034633	7	40	from	domains	1264:1270	arg1	border					1285:1290	the brush border	1275:1290	the brush border	1275:1290	Collectively, the results showed that FITC-GC binds passively to lipid raft domains in the brush border, i.e. without inducing endocytosis like CTB.
28034633	8	41	theme	lipid	1435:1439	arg1	organization					1446:1457	the lipid raft organization	1431:1457	the lipid raft organization of the brush border	1431:1477	Instead, and unlike CTB, FITC-GC seems to exert a stabilizing, detergent-protective effect on the lipid raft organization of the brush border.
28034633	5	42	theme	simultaneous	991:1002	arg1	presence					1004:1011	the simultaneous presence	987:1011	the simultaneous presence of cholera toxin B subunit (CTB)	987:1044	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	8	43	theme	brush	1466:1470	arg1	border					1472:1477	the brush border	1462:1477	the brush border	1462:1477	Instead, and unlike CTB, FITC-GC seems to exert a stabilizing, detergent-protective effect on the lipid raft organization of the brush border.
28034633	2	44	theme	chitosan	365:372	arg1	derivative					351:360	a soluble derivative	341:360	a soluble derivative of chitosan	341:372	Recently, glycol chitosan (GC), a soluble derivative of chitosan, was shown to bind specifically to lipid raft domains in model bilayers.
28034633	2	44	theme	chitosan	365:372	arg1	chitosan					326:333	glycol chitosan	319:333	glycol chitosan (GC)	319:338	Recently, glycol chitosan (GC), a soluble derivative of chitosan, was shown to bind specifically to lipid raft domains in model bilayers.
28034633	2	45	theme	raft	415:418	arg1	domains					420:426	lipid raft domains	409:426	lipid raft domains in model bilayers	409:444	Recently, glycol chitosan (GC), a soluble derivative of chitosan, was shown to bind specifically to lipid raft domains in model bilayers.
28034633	3	46	theme	small	696:700	arg1	epithelium					713:722	the small intestinal epithelium	692:722	the small intestinal epithelium	692:722	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	6	47	theme	density	1131:1137	arg1	profile					1139:1145	the density profile	1127:1145	the density profile of detergent resistant membranes (DRMs)	1127:1185	Biochemically, GC bound to microvillus membrane vesicles and caused a change in the density profile of detergent resistant membranes (DRMs).
28034633	2	48	theme	lipid	409:413	arg1	domains					420:426	lipid raft domains	409:426	lipid raft domains in model bilayers	409:444	Recently, glycol chitosan (GC), a soluble derivative of chitosan, was shown to bind specifically to lipid raft domains in model bilayers.
28034633	1	49	theme	transmucosal	281:292	arg1	delivery					299:306	transmucosal drug delivery	281:306	transmucosal drug delivery	281:306	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	4	50	theme	widespread	808:817	arg1	binding					819:825	widespread binding	808:825	widespread binding of luminal FITC-GC to the brush border	808:864	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	4	51	theme	jejunal	752:758	arg1	explants					768:775	pig jejunal mucosal explants	748:775	pig jejunal mucosal explants	748:775	Using organ culture of pig jejunal mucosal explants as a model system, we observed widespread binding of luminal FITC-GC to the brush border.
28034633	5	52	theme	B	1030:1030	arg1	CTB					1041:1043	CTB	1041:1043	CTB	1041:1043	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	5	52	theme	B	1030:1030	arg1	subunit					1032:1038	cholera toxin B subunit	1016:1038	cholera toxin B subunit (CTB)	1016:1044	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	2	53	theme	glycol	319:324	arg1	derivative					351:360	a soluble derivative	341:360	a soluble derivative of chitosan	341:372	Recently, glycol chitosan (GC), a soluble derivative of chitosan, was shown to bind specifically to lipid raft domains in model bilayers.
28034633	2	53	theme	glycol	319:324	arg1	GC					336:337	GC	336:337	GC	336:337	Recently, glycol chitosan (GC), a soluble derivative of chitosan, was shown to bind specifically to lipid raft domains in model bilayers.
28034633	2	53	theme	glycol	319:324	arg1	chitosan					326:333	glycol chitosan	319:333	glycol chitosan (GC)	319:338	Recently, glycol chitosan (GC), a soluble derivative of chitosan, was shown to bind specifically to lipid raft domains in model bilayers.
28034633	0	54	theme	lipid	33:37	arg1	rafts					39:43	lipid rafts	33:43	lipid rafts	33:43	Glycol chitosan: A stabilizer of lipid rafts in the intestinal brush border.
28034633	1	55	theme	drug	294:297	arg1	delivery					299:306	transmucosal drug delivery	281:306	transmucosal drug delivery	281:306	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	3	56	theme	present	603:609	arg1	work					611:614	the present work	599:614	the present work	599:614	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	3	57	theme	high	531:534	arg1	amounts					536:542	high amounts	531:542	high amounts of glycolipids cross-linked by lectins	531:581	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	3	57	theme	high	531:534	arg1	glycolipids					547:557	glycolipids	547:557	glycolipids cross-linked by lectins	547:581	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	1	58	theme	polycationic	91:102	arg1	polysaccharide					104:117	a polycationic polysaccharide	89:117	a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units	89:164	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	1	58	theme	polycationic	91:102	arg1	Chitosan					77:84	Chitosan	77:84	Chitosan	77:84	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	3	59	contain	has	490:492	arg2	composition					514:524	a unique lipid raft composition	494:524	a unique lipid raft composition	494:524	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	3	59	contain	has	490:492	arg1	membrane					481:488	The small intestinal brush border membrane	447:488	The small intestinal brush border membrane	447:488	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	3	60	theme	work	611:614	arg1	aim					592:594	the aim	588:594	the aim of the present work	588:614	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	7	61	theme	lipid	1253:1257	arg1	domains					1264:1270	lipid raft domains	1253:1270	lipid raft domains	1253:1270	Collectively, the results showed that FITC-GC binds passively to lipid raft domains in the brush border, i.e. without inducing endocytosis like CTB.
28034633	7	62	theme	brush	1279:1283	arg1	border					1285:1290	the brush border	1275:1290	the brush border	1275:1290	Collectively, the results showed that FITC-GC binds passively to lipid raft domains in the brush border, i.e. without inducing endocytosis like CTB.
28034633	3	63	with	interaction	643:653	arg1	epithelium					713:722	the small intestinal epithelium	692:722	the small intestinal epithelium	692:722	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	1	64	theme	mucoadhesive	181:192	arg1	properties					194:203	its mucoadhesive properties	177:203	its mucoadhesive properties	177:203	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	8	65	theme	stabilizing	1387:1397	arg1	effect					1421:1426	a stabilizing, detergent-protective effect	1385:1426	a stabilizing, detergent-protective effect	1385:1426	Instead, and unlike CTB, FITC-GC seems to exert a stabilizing, detergent-protective effect on the lipid raft organization of the brush border.
28034633	7	66	theme	raft	1259:1262	arg1	domains					1264:1270	lipid raft domains	1253:1270	lipid raft domains	1253:1270	Collectively, the results showed that FITC-GC binds passively to lipid raft domains in the brush border, i.e. without inducing endocytosis like CTB.
28034633	6	67	theme	microvillus	1074:1084	arg1	vesicles					1095:1102	microvillus membrane vesicles	1074:1102	microvillus membrane vesicles	1074:1102	Biochemically, GC bound to microvillus membrane vesicles and caused a change in the density profile of detergent resistant membranes (DRMs).
28034633	8	68	theme	border	1472:1477	arg1	organization					1446:1457	the lipid raft organization	1431:1457	the lipid raft organization of the brush border	1431:1477	Instead, and unlike CTB, FITC-GC seems to exert a stabilizing, detergent-protective effect on the lipid raft organization of the brush border.
28034633	0	69	dep	chitosan	7:14	arg1	stabilizer					19:28	A stabilizer	17:28	Glycol chitosan: A stabilizer of lipid rafts in the intestinal brush border.	0:75	Glycol chitosan: A stabilizer of lipid rafts in the intestinal brush border.
28034633	3	70	theme	intestinal	457:466	arg1	membrane					481:488	The small intestinal brush border membrane	447:488	The small intestinal brush border membrane	447:488	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	2	71	theme	soluble	343:349	arg1	derivative					351:360	a soluble derivative	341:360	a soluble derivative of chitosan	341:372	Recently, glycol chitosan (GC), a soluble derivative of chitosan, was shown to bind specifically to lipid raft domains in model bilayers.
28034633	2	71	theme	soluble	343:349	arg1	chitosan					326:333	glycol chitosan	319:333	glycol chitosan (GC)	319:338	Recently, glycol chitosan (GC), a soluble derivative of chitosan, was shown to bind specifically to lipid raft domains in model bilayers.
28034633	0	72	theme	rafts	39:43	arg1	stabilizer					19:28	A stabilizer	17:28	Glycol chitosan: A stabilizer of lipid rafts in the intestinal brush border.	0:75	Glycol chitosan: A stabilizer of lipid rafts in the intestinal brush border.
28034633	5	73	theme	early	927:931	arg1	endosomes					933:941	apical early endosomes	920:941	apical early endosomes	920:941	Only little uptake via constitutive endocytosis into apical early endosomes occurred, unless endocytosis was induced by the simultaneous presence of cholera toxin B subunit (CTB).
28034633	3	74	theme	brush	468:472	arg1	membrane					481:488	The small intestinal brush border membrane	447:488	The small intestinal brush border membrane	447:488	The small intestinal brush border membrane has a unique lipid raft composition with high amounts of glycolipids cross-linked by lectins, and the aim of the present work therefore was to study the interaction of FITC-conjugated GC (FITC-GC) with the small intestinal epithelium.
28034633	1	75	theme	chemical	206:213	arg1	candidates					253:262	potential candidates	243:262	potential candidates as enhancers for transmucosal drug delivery	243:306	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
28034633	1	75	theme	chemical	206:213	arg1	derivatives					215:225	chemical derivatives	206:225	chemical derivatives of chitosan	206:237	Chitosan is a polycationic polysaccharide consisting of β-(1-4)-linked glucosamine units and due to its mucoadhesive properties, chemical derivatives of chitosan are potential candidates as enhancers for transmucosal drug delivery.
27271521	0	0	theme	protein	92:98	arg1	expression					100:109	recombinant protein expression	80:109	recombinant protein expression in silkworm larvae	80:128	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	3	1	theme	silkworm	733:740	arg1	reagent					755:761	a conventional silkworm transfection reagent	718:761	a conventional silkworm transfection reagent	718:761	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	3	1	theme	silkworm	733:740	arg1	DMRIE-C					709:715	DMRIE-C	709:715	DMRIE-C	709:715	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	6	2	theme	silkworm	1317:1324	arg1	larvae					1326:1331	silkworm larvae	1317:1331	silkworm larvae	1317:1331	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	4	3	dep	chitosan	798:805	arg1	nanocomplexes					828:840	bacmid DNA nanocomplexes	817:840	bacmid DNA nanocomplexes	817:840	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	5	4	theme	same	1051:1054	arg1	extent					1056:1061	the same extent	1047:1061	the same extent using chitosan as that using DMRIE-C	1047:1098	In addition, three different proteins were expressed in silkworm larvae to the same extent using chitosan as that using DMRIE-C.
27271521	0	5	theme	recombinant	80:90	arg1	expression					100:109	recombinant protein expression	80:109	recombinant protein expression in silkworm larvae	80:128	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	1	6	theme	chitosan	171:178	arg1	feasibility					156:166	the feasibility	152:166	the feasibility of chitosan	152:178	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	1	6	theme	chitosan	171:178	arg1	candidate					219:227	an alternative transfection reagent candidate	183:227	an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA	183:318	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	1	7	theme	silkworm	269:276	arg1	larvae					278:283	silkworm larvae	269:283	silkworm larvae	269:283	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	4	8	theme	larvae	954:959	arg1	hemolymph					961:969	the silkworm larvae hemolymph	941:969	the silkworm larvae hemolymph	941:969	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	2	9	used	used	470:473	arg2	Chitosan					328:335	RESULT Chitosan 100	321:339	RESULT Chitosan 100	321:339	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	2	9	used	used	470:473	arg2	DNA					405:407	recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA	345:407	recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA	345:407	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	6	10	theme	Bm5	1303:1305	arg1	cells					1307:1311	Bm5 cells	1303:1311	Bm5 cells	1303:1311	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	6	11	theme	reagents	1248:1255	arg1	performance					1197:1207	the performance	1193:1207	the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae	1193:1331	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	4	12	theme	chitosan	798:805	arg1	composition					783:793	the composition	779:793	the composition	779:793	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	3	13	theme	specific	578:585	arg1	activity					587:594	higher specific activity	571:594	higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae	571:702	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	0	14	theme	expression	100:109	arg1	reagent					69:75	transfection reagent	56:75	transfection reagent of recombinant protein expression in silkworm larvae	56:128	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	6	15	theme	bacmid	1158:1163	arg1	nanocomplexes					1169:1181	chitosan/BmNPV bacmid DNA nanocomplexes	1143:1181	chitosan/BmNPV bacmid DNA nanocomplexes	1143:1181	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	3	16	theme	higher	571:576	arg1	activity					587:594	higher specific activity	571:594	higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae	571:702	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	4	17	theme	8	868:868	arg1	ratio					859:863	an N/P ratio	852:863	an N/P ratio of 8 or 10, respectively	852:888	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	3	18	theme	bacmid	539:544	arg1	nanocomplexes					550:562	The chitosan/BmNPV bacmid DNA nanocomplexes	520:562	The chitosan/BmNPV bacmid DNA nanocomplexes	520:562	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	0	19	theme	silkworm	114:121	arg1	larvae					123:128	silkworm larvae	114:128	silkworm larvae	114:128	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	3	20	theme	transfection	742:753	arg1	reagent					755:761	a conventional silkworm transfection reagent	718:761	a conventional silkworm transfection reagent	718:761	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	3	20	theme	transfection	742:753	arg1	DMRIE-C					709:715	DMRIE-C	709:715	DMRIE-C	709:715	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	6	21	theme	recombinant	1279:1289	arg1	proteins					1291:1298	recombinant proteins	1279:1298	recombinant proteins	1279:1298	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	6	22	theme	chitosan/BmNPV	1143:1156	arg1	nanocomplexes					1169:1181	chitosan/BmNPV bacmid DNA nanocomplexes	1143:1181	chitosan/BmNPV bacmid DNA nanocomplexes	1143:1181	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	3	23	theme	DNA	546:548	arg1	nanocomplexes					550:562	The chitosan/BmNPV bacmid DNA nanocomplexes	520:562	The chitosan/BmNPV bacmid DNA nanocomplexes	520:562	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	6	24	theme	available	1225:1233	arg1	reagents					1248:1255	commercially available transfection reagents	1212:1255	commercially available transfection reagents	1212:1255	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	6	25	dep	CONCLUSION	1101:1110	arg1	This					1112:1115	This	1112:1115	This	1112:1115	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	6	25	dep	CONCLUSION	1101:1110	arg1	finding					1130:1136	the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae	1120:1331	the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae	1120:1331	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	4	26	theme	10	873:874	arg1	ratio					859:863	an N/P ratio	852:863	an N/P ratio of 8 or 10, respectively	852:888	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	6	27	theme	DNA	1165:1167	arg1	nanocomplexes					1169:1181	chitosan/BmNPV bacmid DNA nanocomplexes	1143:1181	chitosan/BmNPV bacmid DNA nanocomplexes	1143:1181	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	1	28	theme	alternative	186:196	arg1	candidate					219:227	an alternative transfection reagent candidate	183:227	an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA	183:318	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	1	28	theme	alternative	186:196	arg1	feasibility					156:166	the feasibility	152:166	the feasibility of chitosan	152:178	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	1	29	theme	recombinant	291:301	arg1	DNA					316:318	recombinant BmNPV bacmid DNA	291:318	recombinant BmNPV bacmid DNA	291:318	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	4	30	from	activity	919:926	arg1	hemolymph					961:969	the silkworm larvae hemolymph	941:969	the silkworm larvae hemolymph	941:969	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	1	31	theme	transfection	198:209	arg1	candidate					219:227	an alternative transfection reagent candidate	183:227	an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA	183:318	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	1	31	theme	transfection	198:209	arg1	feasibility					156:166	the feasibility	152:166	the feasibility of chitosan	152:178	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	0	32	theme	bacmid	30:35	arg1	nanocomplex					41:51	chitosan/BmNPV bacmid DNA nanocomplex	15:51	chitosan/BmNPV bacmid DNA nanocomplex	15:51	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	1	33	theme	BmNPV	303:307	arg1	DNA					316:318	recombinant BmNPV bacmid DNA	291:318	recombinant BmNPV bacmid DNA	291:318	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	4	34	theme	β3GnT2	931:936	arg1	activity					919:926	the highest specific activity	898:926	the highest specific activity of β3GnT2 in the silkworm larvae hemolymph	898:969	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	6	35	theme	transfection	1235:1246	arg1	reagents					1248:1255	commercially available transfection reagents	1212:1255	commercially available transfection reagents	1212:1255	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	2	36	theme	RESULT	321:326	arg1	Chitosan					328:335	RESULT Chitosan 100	321:339	RESULT Chitosan 100	321:339	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	1	37	theme	reagent	211:217	arg1	candidate					219:227	an alternative transfection reagent candidate	183:227	an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA	183:318	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	1	37	theme	reagent	211:217	arg1	feasibility					156:166	the feasibility	152:166	the feasibility of chitosan	152:178	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	0	38	theme	chitosan/BmNPV	15:28	arg1	nanocomplex					41:51	chitosan/BmNPV bacmid DNA nanocomplex	15:51	chitosan/BmNPV bacmid DNA nanocomplex	15:51	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	1	39	theme	bacmid	309:314	arg1	DNA					316:318	recombinant BmNPV bacmid DNA	291:318	recombinant BmNPV bacmid DNA	291:318	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	4	40	theme	BmNPV	811:815	arg1	composition					783:793	the composition	779:793	the composition	779:793	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	2	41	theme	nucleopolyhedrovirus	369:388	arg1	DNA					405:407	recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA	345:407	recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA	345:407	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	2	42	theme	group	435:439	arg1	ratios					447:452	amino group/phosphate group (N/P) ratios	413:452	amino group/phosphate group (N/P) ratios of 0.1-10	413:462	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	0	43	theme	nanocomplex	41:51	arg1	Versatility					0:10	Versatility	0:10	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.	0:129	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	6	44	theme	first	1124:1128	arg1	This					1112:1115	This	1112:1115	This	1112:1115	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	6	44	theme	first	1124:1128	arg1	finding					1130:1136	the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae	1120:1331	the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae	1120:1331	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	2	45	theme	bacmid	398:403	arg1	DNA					405:407	recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA	345:407	recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA	345:407	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	2	46	theme	group/phosphate	419:433	arg1	ratios					447:452	amino group/phosphate group (N/P) ratios	413:452	amino group/phosphate group (N/P) ratios of 0.1-10	413:462	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	0	47	theme	DNA	37:39	arg1	nanocomplex					41:51	chitosan/BmNPV bacmid DNA nanocomplex	15:51	chitosan/BmNPV bacmid DNA nanocomplex	15:51	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	6	48	from	expression	1265:1274	arg1	cells					1307:1311	Bm5 cells	1303:1311	Bm5 cells	1303:1311	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	6	48	from	expression	1265:1274	arg1	larvae					1326:1331	silkworm larvae	1317:1331	silkworm larvae	1317:1331	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	3	49	theme	β3GnT2	645:650	arg1	GGT2					669:672	GGT2	669:672	GGT2	669:672	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	3	49	theme	β3GnT2	645:650	arg1	protein					660:666	GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein	599:666	GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae	599:702	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	2	50	theme	amino	413:417	arg1	ratios					447:452	amino group/phosphate group (N/P) ratios	413:452	amino group/phosphate group (N/P) ratios of 0.1-10	413:462	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	1	51	theme	protein	233:239	arg1	expression					241:250	protein expression	233:250	protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA	233:318	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	0	52	theme	transfection	56:67	arg1	reagent					69:75	transfection reagent	56:75	transfection reagent of recombinant protein expression in silkworm larvae	56:128	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	4	53	theme	highest	902:908	arg1	activity					919:926	the highest specific activity	898:926	the highest specific activity of β3GnT2 in the silkworm larvae hemolymph	898:969	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	0	54	from	reagent	69:75	arg1	larvae					123:128	silkworm larvae	114:128	silkworm larvae	114:128	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	3	55	theme	GFPuv-β1,3-N-acetylglucosaminyltransferase	599:640	arg1	GGT2					669:672	GGT2	669:672	GGT2	669:672	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	3	55	theme	GFPuv-β1,3-N-acetylglucosaminyltransferase	599:640	arg1	protein					660:666	GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein	599:666	GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae	599:702	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	2	56	theme	mori	364:367	arg1	BmNPV					391:395	BmNPV	391:395	BmNPV	391:395	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	2	56	theme	mori	364:367	arg1	nucleopolyhedrovirus					369:388	Bombyx mori nucleopolyhedrovirus	357:388	recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA	345:407	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	2	57	theme	0.1-10	457:462	arg1	ratios					447:452	amino group/phosphate group (N/P) ratios	413:452	amino group/phosphate group (N/P) ratios of 0.1-10	413:462	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	0	58	from	larvae	123:128	arg1	reagent					69:75	transfection reagent	56:75	transfection reagent of recombinant protein expression in silkworm larvae	56:128	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	4	59	theme	N/P	855:857	arg1	ratio					859:863	an N/P ratio	852:863	an N/P ratio of 8 or 10, respectively	852:888	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	2	60	theme	Bombyx	357:362	arg1	BmNPV					391:395	BmNPV	391:395	BmNPV	391:395	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	2	60	theme	Bombyx	357:362	arg1	nucleopolyhedrovirus					369:388	Bombyx mori nucleopolyhedrovirus	357:388	recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA	345:407	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	3	61	theme	fusion	653:658	arg1	GGT2					669:672	GGT2	669:672	GGT2	669:672	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	3	61	theme	fusion	653:658	arg1	protein					660:666	GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein	599:666	GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae	599:702	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	1	62	from	expression	241:250	arg1	cells					259:263	Bm5 cells	255:263	Bm5 cells	255:263	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	1	62	from	expression	241:250	arg1	larvae					278:283	silkworm larvae	269:283	silkworm larvae	269:283	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	4	63	theme	DNA	824:826	arg1	nanocomplexes					828:840	bacmid DNA nanocomplexes	817:840	bacmid DNA nanocomplexes	817:840	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	3	64	theme	protein	660:666	arg1	activity					587:594	higher specific activity	571:594	higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae	571:702	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	5	65	theme	silkworm	1028:1035	arg1	larvae					1037:1042	silkworm larvae	1028:1042	silkworm larvae to the same extent using chitosan as that using DMRIE-C	1028:1098	In addition, three different proteins were expressed in silkworm larvae to the same extent using chitosan as that using DMRIE-C.
27271521	2	66	theme	recombinant	345:355	arg1	DNA					405:407	recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA	345:407	recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA	345:407	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	2	67	theme	nanocomplexes	505:517	arg1	formation					479:487	formation	479:487	formation of chitosan/DNA nanocomplexes	479:517	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	4	68	theme	specific	910:917	arg1	activity					919:926	the highest specific activity	898:926	the highest specific activity of β3GnT2 in the silkworm larvae hemolymph	898:969	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	3	69	theme	silkworm	688:695	arg1	larvae					697:702	silkworm larvae	688:702	silkworm larvae	688:702	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	4	70	theme	bacmid	817:822	arg1	nanocomplexes					828:840	bacmid DNA nanocomplexes	817:840	bacmid DNA nanocomplexes	817:840	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27271521	2	71	theme	chitosan/DNA	492:503	arg1	nanocomplexes					505:517	chitosan/DNA nanocomplexes	492:517	chitosan/DNA nanocomplexes	492:517	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	0	72	from	expression	100:109	arg1	larvae					123:128	silkworm larvae	114:128	silkworm larvae	114:128	Versatility of chitosan/BmNPV bacmid DNA nanocomplex as transfection reagent of recombinant protein expression in silkworm larvae.
27271521	1	73	theme	Bm5	255:257	arg1	cells					259:263	Bm5 cells	255:263	Bm5 cells	255:263	OBJECTIVE To examine the feasibility of chitosan as an alternative transfection reagent candidate for protein expression in Bm5 cells and silkworm larvae using recombinant BmNPV bacmid DNA.
27271521	5	74	theme	different	991:999	arg1	proteins					1001:1008	three different proteins	985:1008	three different proteins	985:1008	In addition, three different proteins were expressed in silkworm larvae to the same extent using chitosan as that using DMRIE-C.
27271521	3	75	theme	conventional	720:731	arg1	reagent					755:761	a conventional silkworm transfection reagent	718:761	a conventional silkworm transfection reagent	718:761	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	3	75	theme	conventional	720:731	arg1	DMRIE-C					709:715	DMRIE-C	709:715	DMRIE-C	709:715	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	2	76	theme	N/P	442:444	arg1	ratios					447:452	amino group/phosphate group (N/P) ratios	413:452	amino group/phosphate group (N/P) ratios of 0.1-10	413:462	RESULT Chitosan 100 and recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA, in amino group/phosphate group (N/P) ratios of 0.1-10, were used for formation of chitosan/DNA nanocomplexes.
27271521	6	77	theme	proteins	1291:1298	arg1	expression					1265:1274	the expression	1261:1274	the expression of recombinant proteins in Bm5 cells and silkworm larvae	1261:1331	CONCLUSION This is the first finding that chitosan/BmNPV bacmid DNA nanocomplexes can rival the performance of commercially available transfection reagents for the expression of recombinant proteins in Bm5 cells and silkworm larvae.
27271521	3	78	theme	chitosan/BmNPV	524:537	arg1	nanocomplexes					550:562	The chitosan/BmNPV bacmid DNA nanocomplexes	520:562	The chitosan/BmNPV bacmid DNA nanocomplexes	520:562	The chitosan/BmNPV bacmid DNA nanocomplexes showed higher specific activity of GFPuv-β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein (GGT2) expressed in silkworm larvae than DMRIE-C, a conventional silkworm transfection reagent.
27271521	4	79	theme	silkworm	945:952	arg1	larvae					954:959	silkworm larvae	945:959	the silkworm larvae hemolymph	941:969	In particular, the composition of chitosan and BmNPV bacmid DNA nanocomplexes formed by an N/P ratio of 8 or 10, respectively, showed the highest specific activity of β3GnT2 in the silkworm larvae hemolymph.
27049812	7	0	theme	meal	1364:1367	arg1	ingestion					1325:1333	ingestion	1325:1333	ingestion of the arabinoxylan-enriched meal	1325:1367	The here-reported results show how postprandial glucose responses were improved by ingestion of the arabinoxylan-enriched meal.
27049812	2	1	theme	milling	353:359	arg1	process					361:367	the milling process	349:367	the milling process	349:367	In this regard, we tested an arabinoxylan-enriched white bread flour, obtained by a patented process by which the bran extracted from the milling process is enzymatically hydrolyzed in order to separate the soluble fraction fiber from the insoluble fiber.
27049812	4	2	theme	study	943:947	arg1	design					949:954	a double-blind crossover study design	918:954	a double-blind crossover study design	918:954	Subjects acutely consumed arabinoxylan-enriched white bread (weight: 100 g) or isoenergetic control breads, in a double-blind crossover study design.
27049812	0	3	theme	Glucose	82:88	arg1	Response					90:97	Postprandial Glucose Response	69:97	Postprandial Glucose Response in Normoglycemic Subjects	69:123	Effects of a Bioavailable Arabinoxylan-enriched White Bread Flour on Postprandial Glucose Response in Normoglycemic Subjects.
27049812	1	4	theme	carbohydrate	170:181	arg1	metabolism					183:192	carbohydrate metabolism	170:192	carbohydrate metabolism	170:192	The beneficial effects of soluble fibers on carbohydrate metabolism are well documented.
27049812	8	5	theme	bread	1459:1463	arg1	consumption					1422:1432	daily consumption	1416:1432	daily consumption of arabinoxylan-enriched bread	1416:1463	Further studies are needed to clarify whether daily consumption of arabinoxylan-enriched bread will benefit patients with type 2 diabetes mellitus.
27049812	5	6	theme	glucose	964:970	arg1	levels					972:977	Plasma glucose levels	957:977	Plasma glucose levels	957:977	Plasma glucose levels were measured just before bread administration and 30 minutes afterwards.
27049812	0	7	theme	Normoglycemic	102:114	arg1	Subjects					116:123	Normoglycemic Subjects	102:123	Normoglycemic Subjects	102:123	Effects of a Bioavailable Arabinoxylan-enriched White Bread Flour on Postprandial Glucose Response in Normoglycemic Subjects.
27049812	0	8	from	Effects	0:6	arg1	Response					90:97	Postprandial Glucose Response	69:97	Postprandial Glucose Response in Normoglycemic Subjects	69:123	Effects of a Bioavailable Arabinoxylan-enriched White Bread Flour on Postprandial Glucose Response in Normoglycemic Subjects.
27049812	3	9	theme	Index	551:555	arg1	2.5 kg/m					570:577	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	3	10	theme	Department	718:727	arg1	Clinic					686:691	the Dietetics Outpatient Clinic	661:691	the Dietetics Outpatient Clinic of the Internal Medicine Department	661:727	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	4	11	theme	white	855:859	arg1	100 g					876:880	weight: 100 g	868:880	weight: 100 g	868:880	Subjects acutely consumed arabinoxylan-enriched white bread (weight: 100 g) or isoenergetic control breads, in a double-blind crossover study design.
27049812	4	11	theme	white	855:859	arg1	bread					861:865	arabinoxylan-enriched white bread	833:865	arabinoxylan-enriched white bread (weight: 100 g)	833:881	Subjects acutely consumed arabinoxylan-enriched white bread (weight: 100 g) or isoenergetic control breads, in a double-blind crossover study design.
27049812	2	12	from	fiber	464:468	arg1	separate					409:416	separate	409:416	separate	409:416	In this regard, we tested an arabinoxylan-enriched white bread flour, obtained by a patented process by which the bran extracted from the milling process is enzymatically hydrolyzed in order to separate the soluble fraction fiber from the insoluble fiber.
27049812	3	13	theme	Body	541:544	arg1	2.5 kg/m					570:577	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	4	14	theme	weight	868:873	arg1	100 g					876:880	weight: 100 g	868:880	weight: 100 g	868:880	Subjects acutely consumed arabinoxylan-enriched white bread (weight: 100 g) or isoenergetic control breads, in a double-blind crossover study design.
27049812	4	14	theme	weight	868:873	arg1	bread					861:865	arabinoxylan-enriched white bread	833:865	arabinoxylan-enriched white bread (weight: 100 g)	833:881	Subjects acutely consumed arabinoxylan-enriched white bread (weight: 100 g) or isoenergetic control breads, in a double-blind crossover study design.
27049812	3	15	theme	±	643:643	arg1	%					648:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	6	16	theme	glucose	1085:1091	arg1	concentrations					1093:1106	The 30-minute peak postprandial glucose concentrations	1053:1106	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals	1053:1140	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	6	16	theme	glucose	1085:1091	arg1	lower					1161:1165	lower	1161:1165	lower	1161:1165	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	2	17	theme	insoluble	454:462	arg1	fiber					464:468	the insoluble fiber	450:468	the insoluble fiber	450:468	In this regard, we tested an arabinoxylan-enriched white bread flour, obtained by a patented process by which the bran extracted from the milling process is enzymatically hydrolyzed in order to separate the soluble fraction fiber from the insoluble fiber.
27049812	3	18	theme	FM	634:635	arg1	%					648:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	4	19	theme	control	899:905	arg1	breads					907:912	isoenergetic control breads	886:912	isoenergetic control breads	886:912	Subjects acutely consumed arabinoxylan-enriched white bread (weight: 100 g) or isoenergetic control breads, in a double-blind crossover study design.
27049812	1	20	from	effects	141:147	arg1	metabolism					183:192	carbohydrate metabolism	170:192	carbohydrate metabolism	170:192	The beneficial effects of soluble fibers on carbohydrate metabolism are well documented.
27049812	3	21	theme	y	538:538	arg1	2.5 kg/m					570:577	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	6	22	dep	5.2 mg/dL	1220:1228	arg1	<					1233:1233	p < 0.05	1231:1238	p < 0.05	1231:1238	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	6	23	theme	peak	1067:1070	arg1	concentrations					1093:1106	The 30-minute peak postprandial glucose concentrations	1053:1106	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals	1053:1140	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	6	23	theme	peak	1067:1070	arg1	lower					1161:1165	lower	1161:1165	lower	1161:1165	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	3	24	dep	volunteers	509:518	arg1	2.5 kg/m					570:577	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	3	25	theme	Fat	624:626	arg1	%					648:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	8	26	theme	diabetes	1499:1506	arg1	mellitus					1508:1515	type 2 diabetes mellitus	1492:1515	type 2 diabetes mellitus	1492:1515	Further studies are needed to clarify whether daily consumption of arabinoxylan-enriched bread will benefit patients with type 2 diabetes mellitus.
27049812	6	27	theme	control	1182:1188	arg1	meal					1190:1193	the control meal	1178:1193	the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05)	1178:1239	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	6	27	theme	control	1182:1188	arg1	5.2 mg/dL					1220:1228	107±4.6 mg/dL vs. 121 ± 5.2 mg/dL	1196:1228	107±4.6 mg/dL vs. 121 ± 5.2 mg/dL	1196:1228	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	3	28	theme	BMI	558:560	arg1	2.5 kg/m					570:577	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	7	29	theme	postprandial	1277:1288	arg1	responses					1298:1306	postprandial glucose responses	1277:1306	postprandial glucose responses	1277:1306	The here-reported results show how postprandial glucose responses were improved by ingestion of the arabinoxylan-enriched meal.
27049812	3	30	dep	2.5 kg/m	570:577	arg1	%					648:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	0	31	theme	Bioavailable	13:24	arg1	Flour					60:64	a Bioavailable Arabinoxylan-enriched White Bread Flour	11:64	a Bioavailable Arabinoxylan-enriched White Bread Flour	11:64	Effects of a Bioavailable Arabinoxylan-enriched White Bread Flour on Postprandial Glucose Response in Normoglycemic Subjects.
27049812	8	32	theme	Further	1370:1376	arg1	studies					1378:1384	Further studies	1370:1384	Further studies	1370:1384	Further studies are needed to clarify whether daily consumption of arabinoxylan-enriched bread will benefit patients with type 2 diabetes mellitus.
27049812	3	33	theme	Mass	546:549	arg1	2.5 kg/m					570:577	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	2	34	theme	soluble	422:428	arg1	fraction					430:437	the soluble fraction	418:437	the soluble fraction fiber	418:443	In this regard, we tested an arabinoxylan-enriched white bread flour, obtained by a patented process by which the bran extracted from the milling process is enzymatically hydrolyzed in order to separate the soluble fraction fiber from the insoluble fiber.
27049812	2	35	theme	fraction	430:437	arg1	fiber					439:443	the soluble fraction fiber	418:443	the soluble fraction fiber	418:443	In this regard, we tested an arabinoxylan-enriched white bread flour, obtained by a patented process by which the bran extracted from the milling process is enzymatically hydrolyzed in order to separate the soluble fraction fiber from the insoluble fiber.
27049812	7	36	theme	arabinoxylan-enriched	1342:1362	arg1	meal					1364:1367	the arabinoxylan-enriched meal	1338:1367	the arabinoxylan-enriched meal	1338:1367	The here-reported results show how postprandial glucose responses were improved by ingestion of the arabinoxylan-enriched meal.
27049812	0	37	from	Response	90:97	arg1	Subjects					116:123	Normoglycemic Subjects	102:123	Normoglycemic Subjects	102:123	Effects of a Bioavailable Arabinoxylan-enriched White Bread Flour on Postprandial Glucose Response in Normoglycemic Subjects.
27049812	2	38	theme	patented	299:306	arg1	process					308:314	a patented process	297:314	a patented process by which the bran extracted from the milling process is enzymatically hydrolyzed in order to separate the soluble fraction fiber from the insoluble fiber	297:468	In this regard, we tested an arabinoxylan-enriched white bread flour, obtained by a patented process by which the bran extracted from the milling process is enzymatically hydrolyzed in order to separate the soluble fraction fiber from the insoluble fiber.
27049812	8	39	theme	type	1492:1495	arg1	mellitus					1508:1515	type 2 diabetes mellitus	1492:1515	type 2 diabetes mellitus	1492:1515	Further studies are needed to clarify whether daily consumption of arabinoxylan-enriched bread will benefit patients with type 2 diabetes mellitus.
27049812	3	40	theme	34-61	525:529	arg1	2.5 kg/m					570:577	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	0	41	theme	White	48:52	arg1	Flour					60:64	a Bioavailable Arabinoxylan-enriched White Bread Flour	11:64	a Bioavailable Arabinoxylan-enriched White Bread Flour	11:64	Effects of a Bioavailable Arabinoxylan-enriched White Bread Flour on Postprandial Glucose Response in Normoglycemic Subjects.
27049812	3	42	theme	normoglycemic	495:507	arg1	volunteers					509:518	24 healthy normoglycemic volunteers	484:518	24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy	484:804	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	4	43	theme	arabinoxylan-enriched	833:853	arg1	100 g					876:880	weight: 100 g	868:880	weight: 100 g	868:880	Subjects acutely consumed arabinoxylan-enriched white bread (weight: 100 g) or isoenergetic control breads, in a double-blind crossover study design.
27049812	4	43	theme	arabinoxylan-enriched	833:853	arg1	bread					861:865	arabinoxylan-enriched white bread	833:865	arabinoxylan-enriched white bread (weight: 100 g)	833:881	Subjects acutely consumed arabinoxylan-enriched white bread (weight: 100 g) or isoenergetic control breads, in a double-blind crossover study design.
27049812	8	44	theme	daily	1416:1420	arg1	consumption					1422:1432	daily consumption	1416:1432	daily consumption of arabinoxylan-enriched bread	1416:1463	Further studies are needed to clarify whether daily consumption of arabinoxylan-enriched bread will benefit patients with type 2 diabetes mellitus.
27049812	3	45	theme	±	568:568	arg1	2.5 kg/m					570:577	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	6	46	theme	arabinoxylan-enriched	1114:1134	arg1	meals					1136:1140	arabinoxylan-enriched meals	1114:1140	arabinoxylan-enriched meals	1114:1140	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	8	47	with	patients	1478:1485	arg1	mellitus					1508:1515	type 2 diabetes mellitus	1492:1515	type 2 diabetes mellitus	1492:1515	Further studies are needed to clarify whether daily consumption of arabinoxylan-enriched bread will benefit patients with type 2 diabetes mellitus.
27049812	0	48	theme	Arabinoxylan-enriched	26:46	arg1	Flour					60:64	a Bioavailable Arabinoxylan-enriched White Bread Flour	11:64	a Bioavailable Arabinoxylan-enriched White Bread Flour	11:64	Effects of a Bioavailable Arabinoxylan-enriched White Bread Flour on Postprandial Glucose Response in Normoglycemic Subjects.
27049812	3	49	theme	Outpatient	675:684	arg1	Clinic					686:691	the Dietetics Outpatient Clinic	661:691	the Dietetics Outpatient Clinic of the Internal Medicine Department	661:727	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	4	50	theme	isoenergetic	886:897	arg1	breads					907:912	isoenergetic control breads	886:912	isoenergetic control breads	886:912	Subjects acutely consumed arabinoxylan-enriched white bread (weight: 100 g) or isoenergetic control breads, in a double-blind crossover study design.
27049812	3	51	theme	Waist	583:587	arg1	WC					604:605	WC	604:605	WC	604:605	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	3	51	theme	Waist	583:587	arg1	circumference					589:601	Waist circumference	583:601	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	4	52	theme	crossover	933:941	arg1	design					949:954	a double-blind crossover study design	918:954	a double-blind crossover study design	918:954	Subjects acutely consumed arabinoxylan-enriched white bread (weight: 100 g) or isoenergetic control breads, in a double-blind crossover study design.
27049812	0	53	theme	Flour	60:64	arg1	Effects					0:6	Effects	0:6	Effects of a Bioavailable Arabinoxylan-enriched White Bread Flour on Postprandial Glucose Response in Normoglycemic Subjects.	0:124	Effects of a Bioavailable Arabinoxylan-enriched White Bread Flour on Postprandial Glucose Response in Normoglycemic Subjects.
27049812	4	54	theme	double-blind	920:931	arg1	design					949:954	a double-blind crossover study design	918:954	a double-blind crossover study design	918:954	Subjects acutely consumed arabinoxylan-enriched white bread (weight: 100 g) or isoenergetic control breads, in a double-blind crossover study design.
27049812	3	55	theme	circumference	589:601	arg1	%					648:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	7	56	theme	glucose	1290:1296	arg1	responses					1298:1306	postprandial glucose responses	1277:1306	postprandial glucose responses	1277:1306	The here-reported results show how postprandial glucose responses were improved by ingestion of the arabinoxylan-enriched meal.
27049812	5	57	theme	bread	1005:1009	arg1	administration					1011:1024	bread administration	1005:1024	bread administration	1005:1024	Plasma glucose levels were measured just before bread administration and 30 minutes afterwards.
27049812	3	58	theme	healthy	487:493	arg1	volunteers					509:518	24 healthy normoglycemic volunteers	484:518	24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy	484:804	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	0	59	theme	Bread	54:58	arg1	Flour					60:64	a Bioavailable Arabinoxylan-enriched White Bread Flour	11:64	a Bioavailable Arabinoxylan-enriched White Bread Flour	11:64	Effects of a Bioavailable Arabinoxylan-enriched White Bread Flour on Postprandial Glucose Response in Normoglycemic Subjects.
27049812	8	60	theme	arabinoxylan-enriched	1437:1457	arg1	bread					1459:1463	arabinoxylan-enriched bread	1437:1463	arabinoxylan-enriched bread	1437:1463	Further studies are needed to clarify whether daily consumption of arabinoxylan-enriched bread will benefit patients with type 2 diabetes mellitus.
27049812	3	61	theme	±	614:614	arg1	%					648:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	3	62	theme	[Age	520:523	arg1	2.5 kg/m					570:577	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	1	63	theme	beneficial	130:139	arg1	effects					141:147	The beneficial effects	126:147	The beneficial effects of soluble fibers on carbohydrate metabolism	126:192	The beneficial effects of soluble fibers on carbohydrate metabolism are well documented.
27049812	3	64	theme	Dietetics	665:673	arg1	Clinic					686:691	the Dietetics Outpatient Clinic	661:691	the Dietetics Outpatient Clinic of the Internal Medicine Department	661:727	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	6	65	theme	p	1231:1231	arg1	<					1233:1233	p < 0.05	1231:1238	p < 0.05	1231:1238	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	3	66	theme	8.0 cm	616:621	arg1	%					648:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	3	67	theme	Internal	700:707	arg1	Department					718:727	the Internal Medicine Department	696:727	the Internal Medicine Department	696:727	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	6	68	theme	postprandial	1072:1083	arg1	concentrations					1093:1106	The 30-minute peak postprandial glucose concentrations	1053:1106	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals	1053:1140	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	6	68	theme	postprandial	1072:1083	arg1	lower					1161:1165	lower	1161:1165	lower	1161:1165	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	2	69	theme	bread	272:276	arg1	flour					278:282	an arabinoxylan-enriched white bread flour	241:282	an arabinoxylan-enriched white bread flour	241:282	In this regard, we tested an arabinoxylan-enriched white bread flour, obtained by a patented process by which the bran extracted from the milling process is enzymatically hydrolyzed in order to separate the soluble fraction fiber from the insoluble fiber.
27049812	3	70	theme	±	531:531	arg1	2.5 kg/m					570:577	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	[Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2)	520:580	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	3	71	theme	Medicine	709:716	arg1	Department					718:727	the Internal Medicine Department	696:727	the Internal Medicine Department	696:727	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	0	72	theme	Postprandial	69:80	arg1	Response					90:97	Postprandial Glucose Response	69:97	Postprandial Glucose Response in Normoglycemic Subjects	69:123	Effects of a Bioavailable Arabinoxylan-enriched White Bread Flour on Postprandial Glucose Response in Normoglycemic Subjects.
27049812	5	73	theme	Plasma	957:962	arg1	levels					972:977	Plasma glucose levels	957:977	Plasma glucose levels	957:977	Plasma glucose levels were measured just before bread administration and 30 minutes afterwards.
27049812	2	74	theme	white	266:270	arg1	flour					278:282	an arabinoxylan-enriched white bread flour	241:282	an arabinoxylan-enriched white bread flour	241:282	In this regard, we tested an arabinoxylan-enriched white bread flour, obtained by a patented process by which the bran extracted from the milling process is enzymatically hydrolyzed in order to separate the soluble fraction fiber from the insoluble fiber.
27049812	1	75	theme	soluble	152:158	arg1	fibers					160:165	soluble fibers	152:165	soluble fibers	152:165	The beneficial effects of soluble fibers on carbohydrate metabolism are well documented.
27049812	7	76	theme	here-reported	1246:1258	arg1	results					1260:1266	The here-reported results	1242:1266	The here-reported results	1242:1266	The here-reported results show how postprandial glucose responses were improved by ingestion of the arabinoxylan-enriched meal.
27049812	6	77	theme	30-minute	1057:1065	arg1	concentrations					1093:1106	The 30-minute peak postprandial glucose concentrations	1053:1106	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals	1053:1140	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	6	77	theme	30-minute	1057:1065	arg1	lower					1161:1165	lower	1161:1165	lower	1161:1165	The 30-minute peak postprandial glucose concentrations after arabinoxylan-enriched meals were significantly lower than after the control meal (107±4.6 mg/dL vs. 121 ± 5.2 mg/dL; p < 0.05).
27049812	3	78	theme	Mass	628:631	arg1	%					648:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%	583:648	We recruited 24 healthy normoglycemic volunteers [Age 34-61 ± 12.5 y; Body Mass Index (BMI) 22.1 ± 2.5 kg/m(2); Waist circumference (WC) 84.43 ± 8.0 cm; Fat Mass (FM) 22.7 ± 8.0%] attending the Dietetics Outpatient Clinic of the Internal Medicine Department at IRCCS Policlinico S. Matteo Foundation, University of Pavia, Pavia, Italy.
27049812	2	79	theme	arabinoxylan-enriched	244:264	arg1	flour					278:282	an arabinoxylan-enriched white bread flour	241:282	an arabinoxylan-enriched white bread flour	241:282	In this regard, we tested an arabinoxylan-enriched white bread flour, obtained by a patented process by which the bran extracted from the milling process is enzymatically hydrolyzed in order to separate the soluble fraction fiber from the insoluble fiber.
27049812	1	80	theme	fibers	160:165	arg1	effects					141:147	The beneficial effects	126:147	The beneficial effects of soluble fibers on carbohydrate metabolism	126:192	The beneficial effects of soluble fibers on carbohydrate metabolism are well documented.
26437327	0	0	theme	woody	81:85	arg1	angiosperms					87:97	three woody angiosperms	75:97	three woody angiosperms differentially affected by an extreme summer drought	75:150	Rooting depth, water relations and non-structural carbohydrate dynamics in three woody angiosperms differentially affected by an extreme summer drought.
26437327	3	1	contain	has	496:498	arg2	system					514:519	a deeper root system	500:519	a deeper root system than the other two species	500:546	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	3	1	contain	has	496:498	arg1	mahaleb					488:494	mahaleb	488:494	mahaleb	488:494	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	6	2	theme	significant	1004:1014	arg1	failure					1026:1032	significant hydraulic failure	1004:1032	significant hydraulic failure	1004:1032	In contrast, in Quercus pubescens and Ostrya carpinifolia, decreasing Ψ and NSC were associated with significant hydraulic failure, with spring-to-summer k loss averaging 60%.
26437327	4	3	theme	carbohydrates	670:682	arg1	NSC					693:695	NSC	693:695	NSC	693:695	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	4	3	theme	carbohydrates	670:682	arg1	content					684:690	non-structural carbohydrates content	655:690	non-structural carbohydrates content (NSC)	655:696	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	6	4	theme	decreasing	962:971	arg1	Ψ					973:973	Ψ	973:973	Ψ	973:973	In contrast, in Quercus pubescens and Ostrya carpinifolia, decreasing Ψ and NSC were associated with significant hydraulic failure, with spring-to-summer k loss averaging 60%.
26437327	4	5	theme	non-structural	655:668	arg1	NSC					693:695	NSC	693:695	NSC	693:695	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	4	5	theme	non-structural	655:668	arg1	content					684:690	non-structural carbohydrates content	655:690	non-structural carbohydrates content (NSC)	655:696	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	8	6	from	role	1332:1335	arg1	progression					1366:1376	seasonal progression	1357:1376	seasonal progression of water status, gas exchange and NSC	1357:1414	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	4	7	theme	morphological	554:566	arg1	trait					568:572	This morphological trait	549:572	This morphological trait	549:572	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	4	8	theme	other	744:748	arg1	species					750:756	the other species	740:756	the other species	740:756	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	4	9	theme	P	582:582	arg1	mahaleb					584:590	P mahaleb	582:590	P mahaleb	582:590	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	3	10	theme	other	530:534	arg1	species					540:546	the other two species	526:546	the other two species	526:546	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	6	11	theme	Ostrya	941:946	arg1	carpinifolia					948:959	Ostrya carpinifolia	941:959	Ostrya carpinifolia	941:959	In contrast, in Quercus pubescens and Ostrya carpinifolia, decreasing Ψ and NSC were associated with significant hydraulic failure, with spring-to-summer k loss averaging 60%.
26437327	3	12	from	changes	394:400	arg1	composition					414:424	isotopic composition	405:424	isotopic composition of xylem sap	405:437	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	4	13	theme	water	611:615	arg1	Ψ					628:628	Ψ	628:628	Ψ	628:628	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	4	13	theme	water	611:615	arg1	potential					617:625	higher water potential	604:625	higher water potential (Ψ)	604:629	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	2	14	theme	Ostrya	284:289	arg1	carpinifolia					291:302	Ostrya carpinifolia	284:302	Ostrya carpinifolia	284:302	Quercus pubescens and Ostrya carpinifolia were heavily impacted, while Prunus mahaleb was largely unaffected.
26437327	4	15	theme	exchange	636:643	arg1	rates					645:649	gas exchange rates	632:649	gas exchange rates	632:649	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	6	16	theme	spring-to-summer	1040:1055	arg1	loss					1059:1062	spring-to-summer k loss	1040:1062	spring-to-summer k loss averaging 60%	1040:1076	In contrast, in Quercus pubescens and Ostrya carpinifolia, decreasing Ψ and NSC were associated with significant hydraulic failure, with spring-to-summer k loss averaging 60%.
26437327	7	17	theme	complex	1150:1156	arg1	phenomenon					1158:1167	a complex phenomenon	1148:1167	a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content	1148:1306	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	7	17	theme	complex	1150:1156	arg1	decline					1137:1143	drought-induced tree decline	1116:1143	drought-induced tree decline	1116:1143	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	1	18	theme	extreme	165:171	arg1	drought					180:186	an extreme summer drought	162:186	an extreme summer drought	162:186	In 2012, an extreme summer drought induced species-specific die-back in woody species in Northeastern Italy.
26437327	5	19	theme	favourable	764:773	arg1	water					775:779	More favourable water	759:779	More favourable water	759:779	More favourable water and carbon states allowed relatively stable maintenance of stem hydraulic conductivity (k) throughout the growing season.
26437327	7	20	theme	single	1209:1214	arg1	predictors					1216:1225	single predictors	1209:1225	single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content	1209:1306	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	1	21	theme	summer	173:178	arg1	drought					180:186	an extreme summer drought	162:186	an extreme summer drought	162:186	In 2012, an extreme summer drought induced species-specific die-back in woody species in Northeastern Italy.
26437327	3	22	theme	isotopic	405:412	arg1	composition					414:424	isotopic composition	405:424	isotopic composition of xylem sap	405:437	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	8	23	theme	integrity	1516:1524	arg1	maintenance					1492:1502	the maintenance	1488:1502	the maintenance of vascular integrity	1488:1524	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	8	24	theme	possible	1422:1429	arg1	consequences					1431:1442	possible consequences	1422:1442	possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity	1422:1524	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	4	25	theme	gas	632:634	arg1	rates					645:649	gas exchange rates	632:649	gas exchange rates	632:649	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	3	26	theme	seasonal	385:392	arg1	samples					463:469	deep soil samples	453:469	deep soil samples	453:469	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	3	26	theme	seasonal	385:392	arg1	changes					394:400	seasonal changes	385:400	seasonal changes in isotopic composition of xylem sap	385:437	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	3	26	theme	seasonal	385:392	arg1	rainfall					440:447	rainfall	440:447	rainfall	440:447	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	8	27	theme	vascular	1507:1514	arg1	integrity					1516:1524	vascular integrity	1507:1524	vascular integrity	1507:1524	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	0	28	theme	extreme	129:135	arg1	drought					144:150	an extreme summer drought	126:150	an extreme summer drought	126:150	Rooting depth, water relations and non-structural carbohydrate dynamics in three woody angiosperms differentially affected by an extreme summer drought.
26437327	8	29	theme	seasonal	1357:1364	arg1	progression					1366:1376	seasonal progression	1357:1376	seasonal progression of water status, gas exchange and NSC	1357:1414	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	8	30	theme	rooting	1340:1346	arg1	depth					1348:1352	rooting depth	1340:1352	rooting depth	1340:1352	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	6	31	theme	hydraulic	1016:1024	arg1	failure					1026:1032	significant hydraulic failure	1004:1032	significant hydraulic failure	1004:1032	In contrast, in Quercus pubescens and Ostrya carpinifolia, decreasing Ψ and NSC were associated with significant hydraulic failure, with spring-to-summer k loss averaging 60%.
26437327	5	32	theme	carbon	785:790	arg1	states					792:797	carbon states	785:797	carbon states	785:797	More favourable water and carbon states allowed relatively stable maintenance of stem hydraulic conductivity (k) throughout the growing season.
26437327	6	33	theme	Quercus	919:925	arg1	pubescens					927:935	Quercus pubescens	919:935	Quercus pubescens	919:935	In contrast, in Quercus pubescens and Ostrya carpinifolia, decreasing Ψ and NSC were associated with significant hydraulic failure, with spring-to-summer k loss averaging 60%.
26437327	8	34	theme	exchange	1399:1406	arg1	progression					1366:1376	seasonal progression	1357:1376	seasonal progression of water status, gas exchange and NSC	1357:1414	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	6	35	theme	k	1057:1057	arg1	loss					1059:1062	spring-to-summer k loss	1040:1062	spring-to-summer k loss averaging 60%	1040:1076	In contrast, in Quercus pubescens and Ostrya carpinifolia, decreasing Ψ and NSC were associated with significant hydraulic failure, with spring-to-summer k loss averaging 60%.
26437327	5	36	theme	conductivity	855:866	arg1	maintenance					825:835	relatively stable maintenance	807:835	relatively stable maintenance of stem hydraulic conductivity (k)	807:870	More favourable water and carbon states allowed relatively stable maintenance of stem hydraulic conductivity (k) throughout the growing season.
26437327	1	37	theme	species-specific	196:211	arg1	die-back					213:220	species-specific die-back	196:220	species-specific die-back	196:220	In 2012, an extreme summer drought induced species-specific die-back in woody species in Northeastern Italy.
26437327	3	38	theme	xylem	429:433	arg1	sap					435:437	xylem sap	429:437	xylem sap	429:437	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	8	39	theme	gas	1395:1397	arg1	exchange					1399:1406	gas exchange	1395:1406	gas exchange	1395:1406	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	5	40	theme	hydraulic	845:853	arg1	k					869:869	k	869:869	k	869:869	More favourable water and carbon states allowed relatively stable maintenance of stem hydraulic conductivity (k) throughout the growing season.
26437327	5	40	theme	hydraulic	845:853	arg1	conductivity					855:866	stem hydraulic conductivity	840:866	stem hydraulic conductivity (k)	840:870	More favourable water and carbon states allowed relatively stable maintenance of stem hydraulic conductivity (k) throughout the growing season.
26437327	3	41	theme	soil	458:461	arg1	samples					463:469	deep soil samples	453:469	deep soil samples	453:469	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	3	41	theme	soil	458:461	arg1	changes					394:400	seasonal changes	385:400	seasonal changes in isotopic composition of xylem sap	385:437	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	3	42	theme	sap	435:437	arg1	composition					414:424	isotopic composition	405:424	isotopic composition of xylem sap	405:437	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	0	43	theme	summer	137:142	arg1	drought					144:150	an extreme summer drought	126:150	an extreme summer drought	126:150	Rooting depth, water relations and non-structural carbohydrate dynamics in three woody angiosperms differentially affected by an extreme summer drought.
26437327	7	44	theme	carbohydrate	1287:1298	arg1	content					1300:1306	carbohydrate content	1287:1306	carbohydrate content	1287:1306	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	0	45	theme	water	15:19	arg1	relations					21:29	water relations	15:29	water relations	15:29	Rooting depth, water relations and non-structural carbohydrate dynamics in three woody angiosperms differentially affected by an extreme summer drought.
26437327	5	46	theme	stable	818:823	arg1	maintenance					825:835	relatively stable maintenance	807:835	relatively stable maintenance of stem hydraulic conductivity (k)	807:870	More favourable water and carbon states allowed relatively stable maintenance of stem hydraulic conductivity (k) throughout the growing season.
26437327	0	47	theme	carbohydrate	50:61	arg1	dynamics					63:70	non-structural carbohydrate dynamics	35:70	non-structural carbohydrate dynamics	35:70	Rooting depth, water relations and non-structural carbohydrate dynamics in three woody angiosperms differentially affected by an extreme summer drought.
26437327	5	48	theme	stem	840:843	arg1	k					869:869	k	869:869	k	869:869	More favourable water and carbon states allowed relatively stable maintenance of stem hydraulic conductivity (k) throughout the growing season.
26437327	5	48	theme	stem	840:843	arg1	conductivity					855:866	stem hydraulic conductivity	840:866	stem hydraulic conductivity (k)	840:870	More favourable water and carbon states allowed relatively stable maintenance of stem hydraulic conductivity (k) throughout the growing season.
26437327	7	49	theme	tree	1230:1233	arg1	status					1235:1240	tree status	1230:1240	tree status	1230:1240	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	0	50	theme	non-structural	35:48	arg1	dynamics					63:70	non-structural carbohydrate dynamics	35:70	non-structural carbohydrate dynamics	35:70	Rooting depth, water relations and non-structural carbohydrate dynamics in three woody angiosperms differentially affected by an extreme summer drought.
26437327	8	51	theme	status	1387:1392	arg1	progression					1366:1376	seasonal progression	1357:1376	seasonal progression of water status, gas exchange and NSC	1357:1414	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	7	52	theme	status	1235:1240	arg1	predictors					1216:1225	single predictors	1209:1225	single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content	1209:1306	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	7	53	theme	drought-induced	1116:1130	arg1	phenomenon					1158:1167	a complex phenomenon	1148:1167	a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content	1148:1306	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	7	53	theme	drought-induced	1116:1130	arg1	decline					1137:1143	drought-induced tree decline	1116:1143	drought-induced tree decline	1116:1143	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	8	54	theme	water	1381:1385	arg1	status					1387:1392	water status	1381:1392	water status	1381:1392	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	8	55	theme	depth	1348:1352	arg1	role					1332:1335	the role	1328:1335	the role of rooting depth in seasonal progression of water status, gas exchange and NSC	1328:1414	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	5	56	theme	growing	887:893	arg1	season					895:900	the growing season	883:900	the growing season	883:900	More favourable water and carbon states allowed relatively stable maintenance of stem hydraulic conductivity (k) throughout the growing season.
26437327	1	57	theme	woody	225:229	arg1	species					231:237	woody species	225:237	woody species in Northeastern Italy	225:259	In 2012, an extreme summer drought induced species-specific die-back in woody species in Northeastern Italy.
26437327	1	58	from	species	231:237	arg1	Italy					255:259	Italy	255:259	Italy	255:259	In 2012, an extreme summer drought induced species-specific die-back in woody species in Northeastern Italy.
26437327	2	59	theme	Quercus	262:268	arg1	pubescens					270:278	Quercus pubescens	262:278	Quercus pubescens	262:278	Quercus pubescens and Ostrya carpinifolia were heavily impacted, while Prunus mahaleb was largely unaffected.
26437327	2	60	theme	Prunus	333:338	arg1	mahaleb					340:346	Prunus mahaleb	333:346	Prunus mahaleb	333:346	Quercus pubescens and Ostrya carpinifolia were heavily impacted, while Prunus mahaleb was largely unaffected.
26437327	7	61	theme	predictors	1216:1225	arg1	basis					1200:1204	the basis	1196:1204	the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content	1196:1306	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	8	62	theme	NSC	1412:1414	arg1	progression					1366:1376	seasonal progression	1357:1376	seasonal progression of water status, gas exchange and NSC	1357:1414	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	7	63	theme	hydraulic	1247:1255	arg1	efficiency					1257:1266	hydraulic efficiency	1247:1266	hydraulic efficiency	1247:1266	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	3	64	theme	deeper	502:507	arg1	system					514:519	a deeper root system	500:519	a deeper root system than the other two species	500:546	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	3	65	theme	deep	453:456	arg1	samples					463:469	deep soil samples	453:469	deep soil samples	453:469	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	3	65	theme	deep	453:456	arg1	changes					394:400	seasonal changes	385:400	seasonal changes in isotopic composition of xylem sap	385:437	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
26437327	4	66	theme	higher	604:609	arg1	Ψ					628:628	Ψ	628:628	Ψ	628:628	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	4	66	theme	higher	604:609	arg1	potential					617:625	higher water potential	604:625	higher water potential (Ψ)	604:629	This morphological trait allowed P mahaleb to maintain higher water potential (Ψ), gas exchange rates and non-structural carbohydrates content (NSC) throughout the summer, when compared with the other species.
26437327	8	67	theme	energy-demanding	1448:1463	arg1	mechanisms					1465:1474	energy-demanding mechanisms	1448:1474	energy-demanding mechanisms involved in the maintenance of vascular integrity	1448:1524	Our data highlight the role of rooting depth in seasonal progression of water status, gas exchange and NSC, with possible consequences for energy-demanding mechanisms involved in the maintenance of vascular integrity.
26437327	7	68	theme	tree	1132:1135	arg1	phenomenon					1158:1167	a complex phenomenon	1148:1167	a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content	1148:1306	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	7	68	theme	tree	1132:1135	arg1	decline					1137:1143	drought-induced tree decline	1116:1143	drought-induced tree decline	1116:1143	Our data support the hypothesis that drought-induced tree decline is a complex phenomenon that cannot be modelled on the basis of single predictors of tree status like hydraulic efficiency, vulnerability and carbohydrate content.
26437327	3	69	theme	root	509:512	arg1	system					514:519	a deeper root system	500:519	a deeper root system than the other two species	500:546	By comparing seasonal changes in isotopic composition of xylem sap, rainfall and deep soil samples, we show that P. mahaleb has a deeper root system than the other two species.
25188596	4	0	theme	electrical	905:914	arg1	signals					932:938	the intercoupled biological, chemical, electrical, and mechanical signals	866:938	the intercoupled biological, chemical, electrical, and mechanical signals in the disc	866:950	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	3	1	theme	degeneration	498:509	arg1	progression					463:473	progression	463:473	progression	463:473	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	3	1	theme	degeneration	498:509	arg1	cause					453:457	cause	453:457	cause	453:457	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	4	2	from	changes	711:717	arg1	composition					743:753	extracellular matrix composition	722:753	extracellular matrix composition	722:753	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	4	2	from	changes	711:717	arg1	structure					759:767	structure	759:767	structure	759:767	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	11	3	from	content	1886:1892	arg1	direction					1910:1918	the sagittal direction	1897:1918	the sagittal direction	1897:1918	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	2	4	theme	nutritional	279:289	arg1	level					291:295	nutritional level	279:295	nutritional level	279:295	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	4	5	theme	biological	883:892	arg1	signals					932:938	the intercoupled biological, chemical, electrical, and mechanical signals	866:938	the intercoupled biological, chemical, electrical, and mechanical signals in the disc	866:950	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	4	6	theme	viability	685:693	arg1	losses					670:675	losses	670:675	losses of cell viability and activities	670:708	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	4	6	theme	viability	685:693	arg1	changes					711:717	changes	711:717	changes in extracellular matrix composition and structure	711:767	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	4	7	theme	chemical	895:902	arg1	signals					932:938	the intercoupled biological, chemical, electrical, and mechanical signals	866:938	the intercoupled biological, chemical, electrical, and mechanical signals in the disc	866:950	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	4	8	theme	activities	699:708	arg1	losses					670:675	losses	670:675	losses of cell viability and activities	670:708	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	4	8	theme	activities	699:708	arg1	changes					711:717	changes	711:717	changes in extracellular matrix composition and structure	711:767	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	3	9	theme	disc	587:590	arg1	diseases					613:620	intervertebral disc degeneration-related diseases	572:620	intervertebral disc degeneration-related diseases	572:620	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	10	10	theme	equilibrium	1675:1685	arg1	state					1687:1691	a new equilibrium state	1669:1691	a new equilibrium state	1669:1691	RESULTS Cell density reached equilibrium state in 30 days after reduced nutritional supply at the disc boundary, whereas the PG and water contents reached a new equilibrium state in 55 years.
25188596	11	11	theme	content	1886:1892	arg1	distribution					1867:1878	the distribution	1863:1878	the distribution of PG content in the sagittal direction	1863:1918	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	1	12	theme	STUDY	103:107	arg1	DESIGN					109:114	STUDY DESIGN	103:114	STUDY DESIGN	103:114	STUDY DESIGN Simulate the progression of human disc degeneration.
25188596	4	13	from	signals	932:938	arg1	disc					947:950	the disc	943:950	the disc	943:950	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	6	14	theme	element	1060:1066	arg1	model					1068:1072	A realistic 3-dimensional finite element model	1027:1072	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc	1019:1105	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	9	15	theme	disc	1364:1367	arg1	process					1382:1388	The cell-mediated disc degeneration process	1346:1388	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries	1346:1444	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries was simulated and validated by comparing with experimental results.
25188596	9	15	theme	disc	1364:1367	arg1	simulated					1450:1458	simulated	1450:1458	simulated	1450:1458	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries was simulated and validated by comparing with experimental results.
25188596	4	16	dep	integrity	805:813	arg1	the					788:790	the	788:790	the	788:790	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	6	17	theme	3-dimensional	1039:1051	arg1	model					1068:1072	A realistic 3-dimensional finite element model	1027:1072	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc	1019:1105	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	4	18	theme	extracellular	722:734	arg1	composition					743:753	extracellular matrix composition	722:753	extracellular matrix composition	722:753	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	10	19	theme	equilibrium	1543:1553	arg1	state					1555:1559	equilibrium state	1543:1559	equilibrium state	1543:1559	RESULTS Cell density reached equilibrium state in 30 days after reduced nutritional supply at the disc boundary, whereas the PG and water contents reached a new equilibrium state in 55 years.
25188596	4	20	theme	tissue-level	792:803	arg1	integrity					805:813	tissue-level integrity	792:813	tissue-level integrity	792:813	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	3	21	theme	treatment	547:555	arg1	strategies					557:566	effective treatment strategies	537:566	effective treatment strategies for intervertebral disc degeneration-related diseases	537:620	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	10	22	theme	reduced	1578:1584	arg1	supply					1598:1603	reduced nutritional supply	1578:1603	reduced nutritional supply at the disc boundary	1578:1624	RESULTS Cell density reached equilibrium state in 30 days after reduced nutritional supply at the disc boundary, whereas the PG and water contents reached a new equilibrium state in 55 years.
25188596	9	23	theme	nutritional	1408:1418	arg1	levels					1420:1425	lowered nutritional levels	1400:1425	lowered nutritional levels at disc boundaries	1400:1444	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries was simulated and validated by comparing with experimental results.
25188596	12	24	theme	long-term	1962:1970	arg1	effect					1972:1977	a long-term effect	1960:1977	a long-term effect	1960:1977	CONCLUSION Poor nutritional supply has a long-term effect on disc degeneration.
25188596	2	25	theme	human	358:362	arg1	degeneration					369:380	human disc degeneration	358:380	human disc degeneration using a numerical method	358:405	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	2	26	from	changes	254:260	arg1	density					270:276	cell density	265:276	cell density	265:276	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	2	26	from	changes	254:260	arg1	level					291:295	nutritional level	279:295	nutritional level	279:295	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	2	26	from	changes	254:260	arg1	content					316:322	proteoglycan (PG) content	298:322	proteoglycan (PG) content	298:322	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	2	26	from	changes	254:260	arg1	content					331:337	water content	325:337	water content	325:337	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	2	26	from	changes	254:260	arg1	volume					344:349	volume	344:349	volume	344:349	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	6	27	theme	disc	1102:1105	arg1	model					1068:1072	A realistic 3-dimensional finite element model	1027:1072	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc	1019:1105	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	7	28	theme	constitutive	1223:1234	arg1	based					1270:1274	based	1270:1274	based	1270:1274	The theoretical framework and the constitutive relationships were all biophysics based.
25188596	7	28	theme	constitutive	1223:1234	arg1	relationships					1236:1248	the constitutive relationships	1219:1248	the constitutive relationships	1219:1248	The theoretical framework and the constitutive relationships were all biophysics based.
25188596	12	29	theme	nutritional	1937:1947	arg1	supply					1949:1954	CONCLUSION Poor nutritional supply	1921:1954	CONCLUSION Poor nutritional supply	1921:1954	CONCLUSION Poor nutritional supply has a long-term effect on disc degeneration.
25188596	6	30	theme	human	1081:1085	arg1	disc					1102:1105	the human intervertebral disc	1077:1105	the human intervertebral disc	1077:1105	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	11	31	with	consistent	1796:1805	arg1	results					1816:1822	the results	1812:1822	the results measured in the literature	1812:1849	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	3	32	theme	DATA	430:433	arg1	SUMMARY					408:414	SUMMARY	408:414	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration	408:509	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	2	33	theme	water	325:329	arg1	content					331:337	water content	325:337	water content	325:337	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	7	34	theme	theoretical	1193:1203	arg1	framework					1205:1213	The theoretical framework	1189:1213	The theoretical framework	1189:1213	The theoretical framework and the constitutive relationships were all biophysics based.
25188596	7	34	theme	theoretical	1193:1203	arg1	based					1270:1274	based	1270:1274	based	1270:1274	The theoretical framework and the constitutive relationships were all biophysics based.
25188596	11	35	from	distribution	1867:1878	arg1	direction					1910:1918	the sagittal direction	1897:1918	the sagittal direction	1897:1918	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	6	36	theme	theory	1181:1186	arg1	basis					1128:1132	the basis	1124:1132	the basis of biomechanoelectrochemical continuum mixture theory	1124:1186	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	12	37	theme	CONCLUSION	1921:1930	arg1	supply					1949:1954	CONCLUSION Poor nutritional supply	1921:1954	CONCLUSION Poor nutritional supply	1921:1954	CONCLUSION Poor nutritional supply has a long-term effect on disc degeneration.
25188596	6	38	theme	continuum	1163:1171	arg1	theory					1181:1186	biomechanoelectrochemical continuum mixture theory	1137:1186	biomechanoelectrochemical continuum mixture theory	1137:1186	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	4	39	theme	tissue	630:635	arg1	degeneration					637:648	tissue degeneration	630:648	tissue degeneration	630:648	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	10	40	theme	water	1646:1650	arg1	contents					1652:1659	the PG and water contents	1635:1659	contents	1652:1659	RESULTS Cell density reached equilibrium state in 30 days after reduced nutritional supply at the disc boundary, whereas the PG and water contents reached a new equilibrium state in 55 years.
25188596	11	41	theme	simulated	1710:1718	arg1	consistent					1796:1805	consistent	1796:1805	consistent	1796:1805	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	11	41	theme	simulated	1710:1718	arg1	results					1720:1726	The simulated results	1706:1726	The simulated results for the distributions of PG and water contents within the disc	1706:1789	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	12	42	contain	has	1956:1958	arg1	supply					1949:1954	CONCLUSION Poor nutritional supply	1921:1954	CONCLUSION Poor nutritional supply	1921:1954	CONCLUSION Poor nutritional supply has a long-term effect on disc degeneration.
25188596	12	42	contain	has	1956:1958	arg2	effect					1972:1977	a long-term effect	1960:1977	a long-term effect	1960:1977	CONCLUSION Poor nutritional supply has a long-term effect on disc degeneration.
25188596	9	43	theme	experimental	1492:1503	arg1	results					1505:1511	experimental results	1492:1511	experimental results	1492:1511	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries was simulated and validated by comparing with experimental results.
25188596	3	44	dep	cause	453:457	arg1	the					449:451	the	449:451	the	449:451	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	10	45	theme	PG	1639:1640	arg1	contents					1652:1659	the PG and water contents	1635:1659	contents	1652:1659	RESULTS Cell density reached equilibrium state in 30 days after reduced nutritional supply at the disc boundary, whereas the PG and water contents reached a new equilibrium state in 55 years.
25188596	3	46	theme	disc	493:496	arg1	degeneration					498:509	intervertebral disc degeneration	478:509	intervertebral disc degeneration	478:509	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	2	47	dep	OBJECTIVE	169:177	arg1	was					207:209	was	207:209	was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method	207:405	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	10	48	theme	new	1671:1673	arg1	state					1687:1691	a new equilibrium state	1669:1691	a new equilibrium state	1669:1691	RESULTS Cell density reached equilibrium state in 30 days after reduced nutritional supply at the disc boundary, whereas the PG and water contents reached a new equilibrium state in 55 years.
25188596	3	49	theme	effective	537:545	arg1	strategies					557:566	effective treatment strategies	537:566	effective treatment strategies for intervertebral disc degeneration-related diseases	537:620	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	2	50	theme	PG	312:313	arg1	content					316:322	proteoglycan (PG) content	298:322	proteoglycan (PG) content	298:322	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	11	51	theme	water	1760:1764	arg1	contents					1766:1773	water contents	1760:1773	water contents	1760:1773	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	8	52	theme	experimental	1324:1335	arg1	results					1337:1343	experimental results	1324:1343	experimental results	1324:1343	All the material properties were obtained from experimental results.
25188596	0	53	theme	disc	48:51	arg1	degeneration					53:64	intervertebral disc degeneration	33:64	intervertebral disc degeneration	33:64	Simulation of the progression of intervertebral disc degeneration due to decreased nutritional supply.
25188596	4	54	theme	intercoupled	870:881	arg1	signals					932:938	the intercoupled biological, chemical, electrical, and mechanical signals	866:938	the intercoupled biological, chemical, electrical, and mechanical signals in the disc	866:950	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	4	55	theme	cell	680:683	arg1	viability					685:693	cell viability	680:693	cell viability	680:693	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	11	56	theme	PG	1753:1754	arg1	distributions					1736:1748	the distributions	1732:1748	the distributions of PG and water contents within the disc	1732:1789	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	10	57	theme	Cell	1522:1525	arg1	density					1527:1533	Cell density	1522:1533	Cell density	1522:1533	RESULTS Cell density reached equilibrium state in 30 days after reduced nutritional supply at the disc boundary, whereas the PG and water contents reached a new equilibrium state in 55 years.
25188596	3	58	theme	intervertebral	572:585	arg1	diseases					613:620	intervertebral disc degeneration-related diseases	572:620	intervertebral disc degeneration-related diseases	572:620	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	11	59	theme	PG	1883:1884	arg1	content					1886:1892	PG content	1883:1892	PG content in the sagittal direction	1883:1918	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	2	60	theme	proteoglycan	298:309	arg1	content					316:322	proteoglycan (PG) content	298:322	proteoglycan (PG) content	298:322	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	4	61	from	losses	670:675	arg1	composition					743:753	extracellular matrix composition	722:753	extracellular matrix composition	722:753	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	4	61	from	losses	670:675	arg1	structure					759:767	structure	759:767	structure	759:767	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	10	62	from	boundary	1617:1624	arg1	supply					1598:1603	reduced nutritional supply	1578:1603	reduced nutritional supply at the disc boundary	1578:1624	RESULTS Cell density reached equilibrium state in 30 days after reduced nutritional supply at the disc boundary, whereas the PG and water contents reached a new equilibrium state in 55 years.
25188596	11	63	theme	sagittal	1901:1908	arg1	direction					1910:1918	the sagittal direction	1897:1918	the sagittal direction	1897:1918	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	3	64	theme	degeneration-related	592:611	arg1	diseases					613:620	intervertebral disc degeneration-related diseases	572:620	intervertebral disc degeneration-related diseases	572:620	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	8	65	theme	material	1285:1292	arg1	properties					1294:1303	All the material properties	1277:1303	All the material properties	1277:1303	All the material properties were obtained from experimental results.
25188596	4	66	theme	mechanical	921:930	arg1	signals					932:938	the intercoupled biological, chemical, electrical, and mechanical signals	866:938	the intercoupled biological, chemical, electrical, and mechanical signals in the disc	866:950	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	4	67	theme	matrix	736:741	arg1	composition					743:753	extracellular matrix composition	722:753	extracellular matrix composition	722:753	During tissue degeneration, the disc undergoes losses of cell viability and activities, changes in extracellular matrix composition and structure, and compromise of the tissue-level integrity and function, which is significantly influenced by the intercoupled biological, chemical, electrical, and mechanical signals in the disc.
25188596	2	68	theme	cell	265:268	arg1	density					270:276	cell density	265:276	cell density	265:276	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	9	69	theme	cell-mediated	1350:1362	arg1	process					1382:1388	The cell-mediated disc degeneration process	1346:1388	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries	1346:1444	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries was simulated and validated by comparing with experimental results.
25188596	9	69	theme	cell-mediated	1350:1362	arg1	simulated					1450:1458	simulated	1450:1458	simulated	1450:1458	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries was simulated and validated by comparing with experimental results.
25188596	1	70	theme	human	144:148	arg1	degeneration					155:166	human disc degeneration	144:166	human disc degeneration	144:166	STUDY DESIGN Simulate the progression of human disc degeneration.
25188596	6	71	theme	finite	1053:1058	arg1	model					1068:1072	A realistic 3-dimensional finite element model	1027:1072	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc	1019:1105	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	6	72	dep	METHODS	1019:1025	arg1	model					1068:1072	A realistic 3-dimensional finite element model	1027:1072	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc	1019:1105	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	0	73	theme	nutritional	83:93	arg1	supply					95:100	nutritional supply	83:100	nutritional supply	83:100	Simulation of the progression of intervertebral disc degeneration due to decreased nutritional supply.
25188596	9	74	theme	degeneration	1369:1380	arg1	process					1382:1388	The cell-mediated disc degeneration process	1346:1388	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries	1346:1444	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries was simulated and validated by comparing with experimental results.
25188596	9	74	theme	degeneration	1369:1380	arg1	simulated					1450:1458	simulated	1450:1458	simulated	1450:1458	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries was simulated and validated by comparing with experimental results.
25188596	1	75	theme	degeneration	155:166	arg1	progression					129:139	the progression	125:139	the progression of human disc degeneration	125:166	STUDY DESIGN Simulate the progression of human disc degeneration.
25188596	6	76	theme	realistic	1029:1037	arg1	model					1068:1072	A realistic 3-dimensional finite element model	1027:1072	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc	1019:1105	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	9	77	theme	lowered	1400:1406	arg1	levels					1420:1425	lowered nutritional levels	1400:1425	lowered nutritional levels at disc boundaries	1400:1444	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries was simulated and validated by comparing with experimental results.
25188596	9	78	from	boundaries	1435:1444	arg1	levels					1420:1425	lowered nutritional levels	1400:1425	lowered nutritional levels at disc boundaries	1400:1444	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries was simulated and validated by comparing with experimental results.
25188596	11	79	from	direction	1910:1918	arg1	distribution					1867:1878	the distribution	1863:1878	the distribution of PG content in the sagittal direction	1863:1918	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	2	80	theme	disc	364:367	arg1	degeneration					369:380	human disc degeneration	358:380	human disc degeneration using a numerical method	358:405	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	12	81	theme	disc	1982:1985	arg1	degeneration					1987:1998	disc degeneration	1982:1998	disc degeneration	1982:1998	CONCLUSION Poor nutritional supply has a long-term effect on disc degeneration.
25188596	0	82	theme	progression	18:28	arg1	Simulation					0:9	Simulation	0:9	Simulation of the progression of intervertebral disc degeneration due to	0:71	Simulation of the progression of intervertebral disc degeneration due to decreased nutritional supply.
25188596	9	83	theme	disc	1430:1433	arg1	boundaries					1435:1444	disc boundaries	1430:1444	disc boundaries	1430:1444	The cell-mediated disc degeneration process caused by lowered nutritional levels at disc boundaries was simulated and validated by comparing with experimental results.
25188596	5	84	theme	human	985:989	arg1	discs					991:995	human discs	985:995	human discs	985:995	Characterizing these signals in human discs in vivo is difficult.
25188596	6	85	theme	intervertebral	1087:1100	arg1	disc					1102:1105	the human intervertebral disc	1077:1105	the human intervertebral disc	1077:1105	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	2	86	theme	numerical	390:398	arg1	method					400:405	a numerical method	388:405	a numerical method	388:405	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	0	87	theme	intervertebral	33:46	arg1	degeneration					53:64	intervertebral disc degeneration	33:64	intervertebral disc degeneration	33:64	Simulation of the progression of intervertebral disc degeneration due to decreased nutritional supply.
25188596	10	88	theme	nutritional	1586:1596	arg1	supply					1598:1603	reduced nutritional supply	1578:1603	reduced nutritional supply at the disc boundary	1578:1624	RESULTS Cell density reached equilibrium state in 30 days after reduced nutritional supply at the disc boundary, whereas the PG and water contents reached a new equilibrium state in 55 years.
25188596	11	89	theme	contents	1766:1773	arg1	distributions					1736:1748	the distributions	1732:1748	the distributions of PG and water contents within the disc	1732:1789	The simulated results for the distributions of PG and water contents within the disc were consistent with the results measured in the literature, except for the distribution of PG content in the sagittal direction.
25188596	0	90	theme	degeneration	53:64	arg1	progression					18:28	the progression	14:28	the progression of intervertebral disc degeneration due to	14:71	Simulation of the progression of intervertebral disc degeneration due to decreased nutritional supply.
25188596	1	91	theme	disc	150:153	arg1	degeneration					155:166	human disc degeneration	144:166	human disc degeneration	144:166	STUDY DESIGN Simulate the progression of human disc degeneration.
25188596	3	92	theme	intervertebral	478:491	arg1	degeneration					498:509	intervertebral disc degeneration	478:509	intervertebral disc degeneration	478:509	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	10	93	dep	RESULTS	1514:1520	arg1	reached					1535:1541	reached	1535:1541	reached	1535:1541	RESULTS Cell density reached equilibrium state in 30 days after reduced nutritional supply at the disc boundary, whereas the PG and water contents reached a new equilibrium state in 55 years.
25188596	12	94	theme	Poor	1932:1935	arg1	supply					1949:1954	CONCLUSION Poor nutritional supply	1921:1954	CONCLUSION Poor nutritional supply	1921:1954	CONCLUSION Poor nutritional supply has a long-term effect on disc degeneration.
25188596	3	95	dep	DATA	430:433	arg1	Understanding					435:447	Understanding	435:447	Understanding	435:447	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
25188596	6	96	theme	mixture	1173:1179	arg1	theory					1181:1186	biomechanoelectrochemical continuum mixture theory	1137:1186	biomechanoelectrochemical continuum mixture theory	1137:1186	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	10	97	theme	disc	1612:1615	arg1	boundary					1617:1624	the disc boundary	1608:1624	the disc boundary	1608:1624	RESULTS Cell density reached equilibrium state in 30 days after reduced nutritional supply at the disc boundary, whereas the PG and water contents reached a new equilibrium state in 55 years.
25188596	2	98	theme	study	201:205	arg1	objective					183:191	The objective	179:191	The objective of this study	179:205	OBJECTIVE The objective of this study was to quantitatively analyze and simulate the changes in cell density, nutritional level, proteoglycan (PG) content, water content, and volume during human disc degeneration using a numerical method.
25188596	5	99	from	signals	974:980	arg1	discs					991:995	human discs	985:995	human discs	985:995	Characterizing these signals in human discs in vivo is difficult.
25188596	6	100	theme	biomechanoelectrochemical	1137:1161	arg1	theory					1181:1186	biomechanoelectrochemical continuum mixture theory	1137:1186	biomechanoelectrochemical continuum mixture theory	1137:1186	METHODS A realistic 3-dimensional finite element model of the human intervertebral disc was developed on the basis of biomechanoelectrochemical continuum mixture theory.
25188596	3	101	theme	BACKGROUND	419:428	arg1	DATA					430:433	BACKGROUND DATA	419:433	BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration	419:509	SUMMARY OF BACKGROUND DATA Understanding the cause and progression of intervertebral disc degeneration is crucial for developing effective treatment strategies for intervertebral disc degeneration-related diseases.
24952326	9	0	from	low	1430:1432	arg1	lipids					1449:1454	lipids	1449:1454	lipids	1449:1454	Workers lived longest on a diet high in carbohydrates and low in protein and lipids.
24952326	9	0	from	low	1430:1432	arg1	protein					1437:1443	protein	1437:1443	protein	1437:1443	Workers lived longest on a diet high in carbohydrates and low in protein and lipids.
24952326	6	1	theme	worker	1108:1113	arg1	lifespan					1098:1105	lifespan	1098:1105	lifespan	1098:1105	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	6	1	theme	worker	1108:1113	arg1	weight					1115:1120	worker weight	1108:1120	worker weight	1108:1120	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	6	1	theme	worker	1108:1113	arg1	sensitivity					1136:1146	gustatory sensitivity	1126:1146	gustatory sensitivity to sugar	1126:1155	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	6	1	theme	worker	1108:1113	arg1	measures					1160:1167	measures	1160:1167	measures of individual physical condition	1160:1200	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	9	2	from	carbohydrates	1412:1424	arg1	high					1404:1407	high	1404:1407	high	1404:1407	Workers lived longest on a diet high in carbohydrates and low in protein and lipids.
24952326	6	3	theme	physical	1183:1190	arg1	condition					1192:1200	individual physical condition	1172:1200	individual physical condition	1172:1200	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	12	4	theme	honey	1840:1844	arg1	health					1850:1855	individual honey bee health	1829:1855	individual honey bee health	1829:1855	These results support a role for AmIlp1 in nutritional homeostasis and provide new insight into how unbalanced diets impact individual honey bee health.
24952326	9	5	from	high	1404:1407	arg1	carbohydrates					1412:1424	carbohydrates	1412:1424	carbohydrates	1412:1424	Workers lived longest on a diet high in carbohydrates and low in protein and lipids.
24952326	4	6	theme	Honey	625:629	arg1	bees					631:634	Honey bees	625:634	Honey bees	625:634	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	2	7	theme	Apis	274:277	arg1	bees					268:271	honey bees	262:271	honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture	262:372	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	2	7	theme	Apis	274:277	arg1	mellifera					279:287	Apis mellifera	274:287	Apis mellifera	274:287	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	1	8	theme	dietary	169:175	arg1	balance					177:183	a healthy dietary balance	159:183	a healthy dietary balance in humans	159:193	The rise in metabolic disorders in the past decades has heightened focus on achieving a healthy dietary balance in humans.
24952326	2	9	from	effects	324:330	arg1	productivity					346:357	productivity	346:357	productivity	346:357	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	2	9	from	effects	324:330	arg1	health					335:340	health	335:340	health	335:340	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	5	10	theme	insulin	997:1003	arg1	IRS					1025:1027	IRS	1025:1027	IRS	1025:1027	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	5	10	theme	insulin	997:1003	arg1	substrate					1014:1022	the insulin receptor substrate	993:1022	the insulin receptor substrate (IRS)	993:1028	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	2	11	theme	bees	268:271	arg1	management					248:257	the management	244:257	the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture	244:372	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	12	12	theme	unbalanced	1805:1814	arg1	diets					1816:1820	unbalanced diets	1805:1820	unbalanced diets	1805:1820	These results support a role for AmIlp1 in nutritional homeostasis and provide new insight into how unbalanced diets impact individual honey bee health.
24952326	7	13	theme	high	1297:1300	arg1	diet					1292:1295	a diet	1290:1295	a diet high in protein	1290:1311	We found that expression of AmIlp1 was affected by diet composition and was highest on a diet high in protein.
24952326	5	14	theme	substrate	1014:1022	arg1	expression					959:968	expression	959:968	expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients	959:1078	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	4	15	theme	nutritional	842:852	arg1	status					854:859	nutritional status	842:859	nutritional status	842:859	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	11	16	theme	greater	1679:1685	arg1	levels					1687:1692	greater levels	1679:1692	greater levels of hunger	1679:1702	Bees fed the high carbohydrates diet were also more responsive to sugar, potentially indicating greater levels of hunger.
24952326	5	17	theme	AmIlp2	981:986	arg1	expression					959:968	expression	959:968	expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients	959:1078	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	3	18	theme	IIS	542:544	arg1	pathway					547:553	the insulin/insulin-like signaling (IIS) pathway	506:553	the insulin/insulin-like signaling (IIS) pathway	506:553	As in other organisms, the insulin/insulin-like signaling (IIS) pathway is likely involved in maintaining nutrient homeostasis in honey bees.
24952326	1	19	from	balance	177:183	arg1	humans					188:193	humans	188:193	humans	188:193	The rise in metabolic disorders in the past decades has heightened focus on achieving a healthy dietary balance in humans.
24952326	7	20	from	protein	1305:1311	arg1	high					1297:1300	high	1297:1300	high	1297:1300	We found that expression of AmIlp1 was affected by diet composition and was highest on a diet high in protein.
24952326	3	21	theme	other	489:493	arg1	organisms					495:503	other organisms	489:503	other organisms	489:503	As in other organisms, the insulin/insulin-like signaling (IIS) pathway is likely involved in maintaining nutrient homeostasis in honey bees.
24952326	9	22	theme	high	1404:1407	arg1	diet					1399:1402	a diet	1397:1402	a diet high in carbohydrates and low in protein and lipids	1397:1454	Workers lived longest on a diet high in carbohydrates and low in protein and lipids.
24952326	5	23	theme	caged	869:873	arg1	bees					882:885	caged worker bees	869:885	caged worker bees artificial diets high in carbohydrates, proteins or lipids	869:944	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	11	24	theme	high	1596:1599	arg1	diet					1615:1618	the high carbohydrates diet	1592:1618	the high carbohydrates diet	1592:1618	Bees fed the high carbohydrates diet were also more responsive to sugar, potentially indicating greater levels of hunger.
24952326	0	25	theme	bee	60:62	arg1	workers					64:70	honey bee workers	54:70	honey bee workers	54:70	Insulin-like peptide response to nutritional input in honey bee workers.
24952326	1	26	theme	heightened	129:138	arg1	focus					140:144	heightened focus	129:144	heightened focus	129:144	The rise in metabolic disorders in the past decades has heightened focus on achieving a healthy dietary balance in humans.
24952326	0	27	from	response	21:28	arg1	workers					64:70	honey bee workers	54:70	honey bee workers	54:70	Insulin-like peptide response to nutritional input in honey bee workers.
24952326	4	28	theme	insulin-like	645:656	arg1	Ilps					668:671	Ilps	668:671	Ilps	668:671	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	4	28	theme	insulin-like	645:656	arg1	peptides					658:665	two insulin-like peptides	641:665	two insulin-like peptides (Ilps)	641:672	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	9	29	theme	low	1430:1432	arg1	diet					1399:1402	a diet	1397:1402	a diet high in carbohydrates and low in protein and lipids	1397:1454	Workers lived longest on a diet high in carbohydrates and low in protein and lipids.
24952326	2	30	contain	has	311:313	arg1	nutrition					301:309	poor nutrition	296:309	poor nutrition	296:309	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	2	30	contain	has	311:313	arg2	effects					324:330	negative effects	315:330	negative effects on health and productivity	315:357	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	5	31	theme	worker	875:880	arg1	bees					882:885	caged worker bees	869:885	caged worker bees artificial diets high in carbohydrates, proteins or lipids	869:944	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	2	32	theme	bee	466:468	arg1	populations					470:480	honey bee populations	460:480	honey bee populations	460:480	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	4	33	theme	fat	724:726	arg1	body					728:731	the fat body	720:731	the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status	720:859	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	10	34	theme	high	1530:1533	arg1	diet					1525:1528	a diet	1523:1528	a diet high in protein and low in carbohydrates and lipids	1523:1580	However, bees fed this diet weighed less than those that received a diet high in protein and low in carbohydrates and lipids.
24952326	0	35	theme	Insulin-like	0:11	arg1	response					21:28	Insulin-like peptide response	0:28	Insulin-like peptide response to nutritional input in honey bee workers.	0:71	Insulin-like peptide response to nutritional input in honey bee workers.
24952326	5	36	theme	artificial	887:896	arg1	diets					898:902	caged worker bees artificial diets	869:902	caged worker bees artificial diets high in carbohydrates, proteins or lipids	869:944	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	4	37	theme	expression	697:706	arg1	patterns					708:715	spatial expression patterns	689:715	spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status	689:859	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	10	38	theme	low	1550:1552	arg1	diet					1525:1528	a diet	1523:1528	a diet high in protein and low in carbohydrates and lipids	1523:1580	However, bees fed this diet weighed less than those that received a diet high in protein and low in carbohydrates and lipids.
24952326	5	39	theme	high	904:907	arg1	diets					898:902	caged worker bees artificial diets	869:902	caged worker bees artificial diets high in carbohydrates, proteins or lipids	869:944	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	7	40	from	high	1297:1300	arg1	protein					1305:1311	protein	1305:1311	protein	1305:1311	We found that expression of AmIlp1 was affected by diet composition and was highest on a diet high in protein.
24952326	0	41	theme	nutritional	33:43	arg1	input					45:49	nutritional input	33:49	nutritional input	33:49	Insulin-like peptide response to nutritional input in honey bee workers.
24952326	3	42	theme	nutrient	589:596	arg1	homeostasis					598:608	nutrient homeostasis	589:608	nutrient homeostasis in honey bees	589:622	As in other organisms, the insulin/insulin-like signaling (IIS) pathway is likely involved in maintaining nutrient homeostasis in honey bees.
24952326	4	43	theme	lipid	781:785	arg1	metabolism					787:796	lipid metabolism	781:796	lipid metabolism	781:796	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	10	44	from	high	1530:1533	arg1	protein					1538:1544	protein	1538:1544	protein	1538:1544	However, bees fed this diet weighed less than those that received a diet high in protein and low in carbohydrates and lipids.
24952326	1	45	from	disorders	95:103	arg1	decades					117:123	the past decades	108:123	the past decades	108:123	The rise in metabolic disorders in the past decades has heightened focus on achieving a healthy dietary balance in humans.
24952326	10	46	from	protein	1538:1544	arg1	high					1530:1533	high	1530:1533	high	1530:1533	However, bees fed this diet weighed less than those that received a diet high in protein and low in carbohydrates and lipids.
24952326	10	47	from	low	1550:1552	arg1	lipids					1575:1580	lipids	1575:1580	lipids	1575:1580	However, bees fed this diet weighed less than those that received a diet high in protein and low in carbohydrates and lipids.
24952326	10	47	from	low	1550:1552	arg1	carbohydrates					1557:1569	carbohydrates	1557:1569	carbohydrates	1557:1569	However, bees fed this diet weighed less than those that received a diet high in protein and low in carbohydrates and lipids.
24952326	2	48	theme	negative	315:322	arg1	effects					324:330	negative effects	315:330	negative effects on health and productivity	315:357	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	5	49	from	proteins	927:934	arg1	high					904:907	high	904:907	high	904:907	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	10	50	from	carbohydrates	1557:1569	arg1	low					1550:1552	low	1550:1552	low	1550:1552	However, bees fed this diet weighed less than those that received a diet high in protein and low in carbohydrates and lipids.
24952326	4	51	contain	have	636:639	arg1	bees					631:634	Honey bees	625:634	Honey bees	625:634	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	4	51	contain	have	636:639	arg2	Ilps					668:671	Ilps	668:671	Ilps	668:671	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	4	51	contain	have	636:639	arg2	peptides					658:665	two insulin-like peptides	641:665	two insulin-like peptides (Ilps)	641:672	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	2	52	theme	honey	262:266	arg1	bees					268:271	honey bees	262:271	honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture	262:372	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	2	52	theme	honey	262:266	arg1	mellifera					279:287	Apis mellifera	274:287	Apis mellifera	274:287	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	5	53	from	carbohydrates	912:924	arg1	high					904:907	high	904:907	high	904:907	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	4	54	theme	general	821:827	arg1	AmIlp2					804:809	AmIlp2	804:809	AmIlp2	804:809	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	4	54	theme	general	821:827	arg1	indicator					829:837	a more general indicator	814:837	a more general indicator of nutritional status	814:859	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	10	55	from	lipids	1575:1580	arg1	low					1550:1552	low	1550:1552	low	1550:1552	However, bees fed this diet weighed less than those that received a diet high in protein and low in carbohydrates and lipids.
24952326	3	56	from	homeostasis	598:608	arg1	bees					619:622	honey bees	613:622	honey bees	613:622	As in other organisms, the insulin/insulin-like signaling (IIS) pathway is likely involved in maintaining nutrient homeostasis in honey bees.
24952326	6	57	theme	condition	1192:1200	arg1	lifespan					1098:1105	lifespan	1098:1105	lifespan	1098:1105	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	6	57	theme	condition	1192:1200	arg1	weight					1115:1120	worker weight	1108:1120	worker weight	1108:1120	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	6	57	theme	condition	1192:1200	arg1	sensitivity					1136:1146	gustatory sensitivity	1126:1146	gustatory sensitivity to sugar	1126:1155	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	6	57	theme	condition	1192:1200	arg1	measures					1160:1167	measures	1160:1167	measures of individual physical condition	1160:1200	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	1	58	contain	has	125:127	arg2	focus					140:144	heightened focus	129:144	heightened focus	129:144	The rise in metabolic disorders in the past decades has heightened focus on achieving a healthy dietary balance in humans.
24952326	1	58	contain	has	125:127	arg1	rise					77:80	The rise	73:80	The rise in metabolic disorders in the past decades	73:123	The rise in metabolic disorders in the past decades has heightened focus on achieving a healthy dietary balance in humans.
24952326	6	59	theme	individual	1172:1181	arg1	condition					1192:1200	individual physical condition	1172:1200	individual physical condition	1172:1200	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	3	60	theme	insulin/insulin-like	510:529	arg1	pathway					547:553	the insulin/insulin-like signaling (IIS) pathway	506:553	the insulin/insulin-like signaling (IIS) pathway	506:553	As in other organisms, the insulin/insulin-like signaling (IIS) pathway is likely involved in maintaining nutrient homeostasis in honey bees.
24952326	5	61	from	lipids	939:944	arg1	high					904:907	high	904:907	high	904:907	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	1	62	theme	healthy	161:167	arg1	balance					177:183	a healthy dietary balance	159:183	a healthy dietary balance in humans	159:193	The rise in metabolic disorders in the past decades has heightened focus on achieving a healthy dietary balance in humans.
24952326	12	63	theme	individual	1829:1838	arg1	health					1850:1855	individual honey bee health	1829:1855	individual honey bee health	1829:1855	These results support a role for AmIlp1 in nutritional homeostasis and provide new insight into how unbalanced diets impact individual honey bee health.
24952326	3	64	theme	signaling	531:539	arg1	pathway					547:553	the insulin/insulin-like signaling (IIS) pathway	506:553	the insulin/insulin-like signaling (IIS) pathway	506:553	As in other organisms, the insulin/insulin-like signaling (IIS) pathway is likely involved in maintaining nutrient homeostasis in honey bees.
24952326	12	65	from	role	1729:1732	arg1	homeostasis					1760:1770	nutritional homeostasis	1748:1770	nutritional homeostasis	1748:1770	These results support a role for AmIlp1 in nutritional homeostasis and provide new insight into how unbalanced diets impact individual honey bee health.
24952326	5	66	theme	receptor	1005:1012	arg1	IRS					1025:1027	IRS	1025:1027	IRS	1025:1027	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	5	66	theme	receptor	1005:1012	arg1	substrate					1014:1022	the insulin receptor substrate	993:1022	the insulin receptor substrate (IRS)	993:1028	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	11	67	theme	hunger	1697:1702	arg1	levels					1687:1692	greater levels	1679:1692	greater levels of hunger	1679:1702	Bees fed the high carbohydrates diet were also more responsive to sugar, potentially indicating greater levels of hunger.
24952326	1	68	theme	past	112:115	arg1	decades					117:123	the past decades	108:123	the past decades	108:123	The rise in metabolic disorders in the past decades has heightened focus on achieving a healthy dietary balance in humans.
24952326	4	69	theme	status	854:859	arg1	AmIlp2					804:809	AmIlp2	804:809	AmIlp2	804:809	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	4	69	theme	status	854:859	arg1	indicator					829:837	a more general indicator	814:837	a more general indicator of nutritional status	814:859	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	6	70	theme	gustatory	1126:1134	arg1	lifespan					1098:1105	lifespan	1098:1105	lifespan	1098:1105	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	6	70	theme	gustatory	1126:1134	arg1	weight					1115:1120	worker weight	1108:1120	worker weight	1108:1120	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	6	70	theme	gustatory	1126:1134	arg1	sensitivity					1136:1146	gustatory sensitivity	1126:1146	gustatory sensitivity to sugar	1126:1155	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	6	70	theme	gustatory	1126:1134	arg1	measures					1160:1167	measures	1160:1167	measures of individual physical condition	1160:1200	We also measured lifespan, worker weight and gustatory sensitivity to sugar as measures of individual physical condition.
24952326	2	71	theme	important	225:233	arg1	This					196:199	This	196:199	This	196:199	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	2	71	theme	important	225:233	arg1	issue					235:239	an increasingly important issue	209:239	an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture	209:372	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	5	72	from	high	904:907	arg1	carbohydrates					912:924	carbohydrates	912:924	carbohydrates	912:924	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	5	72	from	high	904:907	arg1	lipids					939:944	lipids	939:944	lipids	939:944	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	5	72	from	high	904:907	arg1	proteins					927:934	proteins	927:934	proteins	927:934	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	12	73	theme	bee	1846:1848	arg1	health					1850:1855	individual honey bee health	1829:1855	individual honey bee health	1829:1855	These results support a role for AmIlp1 in nutritional homeostasis and provide new insight into how unbalanced diets impact individual honey bee health.
24952326	11	74	theme	carbohydrates	1601:1613	arg1	diet					1615:1618	the high carbohydrates diet	1592:1618	the high carbohydrates diet	1592:1618	Bees fed the high carbohydrates diet were also more responsive to sugar, potentially indicating greater levels of hunger.
24952326	12	75	theme	new	1784:1786	arg1	insight					1788:1794	new insight	1784:1794	new insight into how unbalanced diets impact individual honey bee health	1784:1855	These results support a role for AmIlp1 in nutritional homeostasis and provide new insight into how unbalanced diets impact individual honey bee health.
24952326	9	76	from	lipids	1449:1454	arg1	low					1430:1432	low	1430:1432	low	1430:1432	Workers lived longest on a diet high in carbohydrates and low in protein and lipids.
24952326	5	77	theme	dietary	1057:1063	arg1	macronutrients					1065:1078	dietary macronutrients	1057:1078	dietary macronutrients	1057:1078	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	9	78	from	protein	1437:1443	arg1	low					1430:1432	low	1430:1432	low	1430:1432	Workers lived longest on a diet high in carbohydrates and low in protein and lipids.
24952326	2	79	from	factor	420:425	arg1	global					441:446	global	441:446	global	441:446	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	12	80	theme	nutritional	1748:1758	arg1	homeostasis					1760:1770	nutritional homeostasis	1748:1770	nutritional homeostasis	1748:1770	These results support a role for AmIlp1 in nutritional homeostasis and provide new insight into how unbalanced diets impact individual honey bee health.
24952326	4	81	from	patterns	708:715	arg1	body					728:731	the fat body	720:731	the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status	720:859	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	7	82	theme	diet	1254:1257	arg1	composition					1259:1269	diet composition	1254:1269	diet composition	1254:1269	We found that expression of AmIlp1 was affected by diet composition and was highest on a diet high in protein.
24952326	2	83	theme	honey	460:464	arg1	bee					466:468	honey bee	460:468	honey bee populations	460:480	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	0	84	theme	peptide	13:19	arg1	response					21:28	Insulin-like peptide response	0:28	Insulin-like peptide response to nutritional input in honey bee workers.	0:71	Insulin-like peptide response to nutritional input in honey bee workers.
24952326	5	85	theme	bees	882:885	arg1	diets					898:902	caged worker bees artificial diets	869:902	caged worker bees artificial diets high in carbohydrates, proteins or lipids	869:944	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	1	86	theme	metabolic	85:93	arg1	disorders					95:103	metabolic disorders	85:103	metabolic disorders in the past decades	85:123	The rise in metabolic disorders in the past decades has heightened focus on achieving a healthy dietary balance in humans.
24952326	7	87	theme	AmIlp1	1231:1236	arg1	expression					1217:1226	expression	1217:1226	expression of AmIlp1	1217:1236	We found that expression of AmIlp1 was affected by diet composition and was highest on a diet high in protein.
24952326	4	88	theme	spatial	689:695	arg1	patterns					708:715	spatial expression patterns	689:715	spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status	689:859	Honey bees have two insulin-like peptides (Ilps) with differing spatial expression patterns in the fat body suggesting that AmIlp1 potentially functions in lipid metabolism while AmIlp2 is a more general indicator of nutritional status.
24952326	2	89	from	issue	235:239	arg1	management					248:257	the management	244:257	the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture	244:372	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	0	90	theme	honey	54:58	arg1	workers					64:70	honey bee workers	54:70	honey bee workers	54:70	Insulin-like peptide response to nutritional input in honey bee workers.
24952326	2	91	dep	global	441:446	arg1	declines					448:455	declines	448:455	declines in honey bee populations	448:480	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	5	92	theme	AmIlp1	973:978	arg1	expression					959:968	expression	959:968	expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients	959:1078	We fed caged worker bees artificial diets high in carbohydrates, proteins or lipids and measured expression of AmIlp1, AmIlp2, and the insulin receptor substrate (IRS) to test their responses to dietary macronutrients.
24952326	2	93	theme	contributing	407:418	arg1	factor					420:425	a contributing factor	405:425	a contributing factor in the recent global declines in honey bee populations	405:480	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	2	93	theme	contributing	407:418	arg1	nutrition					379:387	nutrition	379:387	nutrition	379:387	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	2	94	theme	poor	296:299	arg1	nutrition					301:309	poor nutrition	296:309	poor nutrition	296:309	This is also an increasingly important issue in the management of honey bees (Apis mellifera) where poor nutrition has negative effects on health and productivity in agriculture, and nutrition is suggested as a contributing factor in the recent global declines in honey bee populations.
24952326	3	95	theme	honey	613:617	arg1	bees					619:622	honey bees	613:622	honey bees	613:622	As in other organisms, the insulin/insulin-like signaling (IIS) pathway is likely involved in maintaining nutrient homeostasis in honey bees.
24952326	8	96	theme	AmIlp2	1328:1333	arg1	Expression					1314:1323	Expression	1314:1323	Expression of AmIlp2 and AmIRS	1314:1343	Expression of AmIlp2 and AmIRS were not affected by diet.
24952326	1	97	from	rise	77:80	arg1	disorders					95:103	metabolic disorders	85:103	metabolic disorders in the past decades	85:123	The rise in metabolic disorders in the past decades has heightened focus on achieving a healthy dietary balance in humans.
24952326	8	98	theme	AmIRS	1339:1343	arg1	Expression					1314:1323	Expression	1314:1323	Expression of AmIlp2 and AmIRS	1314:1343	Expression of AmIlp2 and AmIRS were not affected by diet.
27315248	0	0	theme	Surface	78:84	arg1	Sensing					86:92	Surface Sensing	78:92	Surface Sensing	78:92	Chitosan Mediates Germling Adhesion in Magnaporthe oryzae and Is Required for Surface Sensing and Germling Morphogenesis.
27315248	6	1	theme	stealth	840:846	arg1	molecule					849:856	a 'stealth' molecule	837:856	a 'stealth' molecule	837:856	Here, it has long been hypothesized to act as a 'stealth' molecule, necessary for full pathogenesis.
27315248	6	1	theme	stealth	840:846	arg1	it					797:798	it	797:798	it	797:798	Here, it has long been hypothesized to act as a 'stealth' molecule, necessary for full pathogenesis.
27315248	14	2	theme	further	2064:2070	arg1	insight					2072:2078	further insight	2064:2078	further insight into the mechanisms governing appressorium development in M.oryzae	2064:2145	This study thus reveals a novel role for chitosan in phytopathogenic fungi, and gives further insight into the mechanisms governing appressorium development in M.oryzae.
27315248	8	3	theme	germ	1046:1049	arg1	tube					1051:1054	the germ tube	1042:1054	the germ tube	1042:1054	We first confirmed that chitosan localizes to the germ tube and appressorium, then deleted CDA genes on the basis of their elevated transcript levels during appressorium differentiation.
27315248	9	4	theme	deletion	1200:1207	arg1	strains					1209:1215	the deletion strains	1196:1215	the deletion strains	1196:1215	Germlings of the deletion strains showed loss of chitin deacetylation, and were compromised in their ability to adhere and form appressoria on artificial hydrophobic surfaces.
27315248	11	5	from	lack	1483:1486	arg1	surfaces					1530:1537	artificial surfaces	1519:1537	artificial surfaces	1519:1537	Despite the lack of appressorium development on artificial surfaces, pathogenicity was unaffected in the mutant strains.
27315248	8	6	theme	elevated	1119:1126	arg1	levels					1139:1144	their elevated transcript levels	1113:1144	their elevated transcript levels during appressorium differentiation	1113:1180	We first confirmed that chitosan localizes to the germ tube and appressorium, then deleted CDA genes on the basis of their elevated transcript levels during appressorium differentiation.
27315248	9	7	theme	strains	1209:1215	arg1	Germlings					1183:1191	Germlings	1183:1191	Germlings of the deletion strains	1183:1215	Germlings of the deletion strains showed loss of chitin deacetylation, and were compromised in their ability to adhere and form appressoria on artificial hydrophobic surfaces.
27315248	9	8	theme	artificial	1326:1335	arg1	surfaces					1349:1356	artificial hydrophobic surfaces	1326:1356	artificial hydrophobic surfaces	1326:1356	Germlings of the deletion strains showed loss of chitin deacetylation, and were compromised in their ability to adhere and form appressoria on artificial hydrophobic surfaces.
27315248	8	9	dep	localizes	1029:1037	arg1	deleted					1079:1085	deleted	1079:1085	deleted CDA genes on the basis of their elevated transcript levels during appressorium differentiation	1079:1180	We first confirmed that chitosan localizes to the germ tube and appressorium, then deleted CDA genes on the basis of their elevated transcript levels during appressorium differentiation.
27315248	13	10	theme	stealth	1796:1802	arg1	role					1785:1788	a role	1783:1788	a role	1783:1788	Thus, chitosan does not have a role as a 'stealth' molecule, but instead mediates the adhesion of germlings to surfaces, thereby allowing the perception of the physical stimuli necessary to promote appressorium development.
27315248	13	10	theme	stealth	1796:1802	arg1	molecule					1805:1812	a 'stealth' molecule	1793:1812	a 'stealth' molecule	1793:1812	Thus, chitosan does not have a role as a 'stealth' molecule, but instead mediates the adhesion of germlings to surfaces, thereby allowing the perception of the physical stimuli necessary to promote appressorium development.
27315248	1	11	theme	cell	133:136	arg1	wall					138:141	The fungal cell wall	122:141	The fungal cell wall	122:141	The fungal cell wall not only plays a critical role in maintaining cellular integrity, but also forms the interface between fungi and their environment.
27315248	14	12	theme	novel	2004:2008	arg1	role					2010:2013	a novel role	2002:2013	a novel role for chitosan in phytopathogenic fungi	2002:2051	This study thus reveals a novel role for chitosan in phytopathogenic fungi, and gives further insight into the mechanisms governing appressorium development in M.oryzae.
27315248	10	13	theme	exogenous	1389:1397	arg1	chitosan					1399:1406	exogenous chitosan	1389:1406	exogenous chitosan	1389:1406	Surprisingly, the addition of exogenous chitosan fully restored germling adhesion and appressorium development.
27315248	8	14	theme	appressorium	1153:1164	arg1	differentiation					1166:1180	appressorium differentiation	1153:1180	appressorium differentiation	1153:1180	We first confirmed that chitosan localizes to the germ tube and appressorium, then deleted CDA genes on the basis of their elevated transcript levels during appressorium differentiation.
27315248	9	15	theme	hydrophobic	1337:1347	arg1	surfaces					1349:1356	artificial hydrophobic surfaces	1326:1356	artificial hydrophobic surfaces	1326:1356	Germlings of the deletion strains showed loss of chitin deacetylation, and were compromised in their ability to adhere and form appressoria on artificial hydrophobic surfaces.
27315248	8	16	theme	transcript	1128:1137	arg1	levels					1139:1144	their elevated transcript levels	1113:1144	their elevated transcript levels during appressorium differentiation	1113:1180	We first confirmed that chitosan localizes to the germ tube and appressorium, then deleted CDA genes on the basis of their elevated transcript levels during appressorium differentiation.
27315248	0	17	theme	Germling	98:105	arg1	Morphogenesis					107:119	Germling Morphogenesis	98:119	Germling Morphogenesis	98:119	Chitosan Mediates Germling Adhesion in Magnaporthe oryzae and Is Required for Surface Sensing and Germling Morphogenesis.
27315248	14	18	theme	phytopathogenic	2031:2045	arg1	fungi					2047:2051	phytopathogenic fungi	2031:2051	phytopathogenic fungi	2031:2051	This study thus reveals a novel role for chitosan in phytopathogenic fungi, and gives further insight into the mechanisms governing appressorium development in M.oryzae.
27315248	11	19	theme	development	1504:1514	arg1	lack					1483:1486	the lack	1479:1486	the lack of appressorium development on artificial surfaces	1479:1537	Despite the lack of appressorium development on artificial surfaces, pathogenicity was unaffected in the mutant strains.
27315248	2	20	theme	wall	303:306	arg1	composition					279:289	The composition	275:289	The composition of the cell wall	275:306	The composition of the cell wall can therefore influence the interactions of fungi with their physical and biological environments.
27315248	12	21	theme	plant	1739:1743	arg1	surface					1745:1751	the plant surface	1735:1751	the plant surface	1735:1751	Further analyses demonstrated that cuticular waxes are sufficient to over-ride the requirement for chitosan during appressorium development on the plant surface.
27315248	1	22	theme	critical	160:167	arg1	role					169:172	a critical role	158:172	a critical role	158:172	The fungal cell wall not only plays a critical role in maintaining cellular integrity, but also forms the interface between fungi and their environment.
27315248	5	23	theme	infection	744:752	arg1	structures					754:763	infection structures	744:763	infection structures	744:763	Chitosan has previously been shown to accumulate in the cell wall of infection structures in phytopathogenic fungi.
27315248	9	24	theme	chitin	1232:1237	arg1	deacetylation					1239:1251	chitin deacetylation	1232:1251	chitin deacetylation	1232:1251	Germlings of the deletion strains showed loss of chitin deacetylation, and were compromised in their ability to adhere and form appressoria on artificial hydrophobic surfaces.
27315248	2	25	theme	cell	298:301	arg1	wall					303:306	the cell wall	294:306	the cell wall	294:306	The composition of the cell wall can therefore influence the interactions of fungi with their physical and biological environments.
27315248	10	26	theme	appressorium	1445:1456	arg1	development					1458:1468	appressorium development	1445:1468	appressorium development	1445:1468	Surprisingly, the addition of exogenous chitosan fully restored germling adhesion and appressorium development.
27315248	5	27	theme	structures	754:763	arg1	wall					736:739	the cell wall	727:739	the cell wall of infection structures in phytopathogenic fungi	727:788	Chitosan has previously been shown to accumulate in the cell wall of infection structures in phytopathogenic fungi.
27315248	9	28	theme	deacetylation	1239:1251	arg1	loss					1224:1227	loss	1224:1227	loss of chitin deacetylation	1224:1251	Germlings of the deletion strains showed loss of chitin deacetylation, and were compromised in their ability to adhere and form appressoria on artificial hydrophobic surfaces.
27315248	4	29	theme	β1,4-glucosamine	657:672	arg1	chitosan					634:641	chitosan	634:641	chitosan	634:641	This reaction is catalyzed by a family of enzymes known as chitin deacetylases (CDAs), and results in the formation of chitosan, a polymer of β1,4-glucosamine.
27315248	4	29	theme	β1,4-glucosamine	657:672	arg1	polymer					646:652	a polymer	644:652	a polymer of β1,4-glucosamine	644:672	This reaction is catalyzed by a family of enzymes known as chitin deacetylases (CDAs), and results in the formation of chitosan, a polymer of β1,4-glucosamine.
27315248	8	30	dep	levels	1139:1144	arg1	basis					1104:1108	basis	1104:1108	basis	1104:1108	We first confirmed that chitosan localizes to the germ tube and appressorium, then deleted CDA genes on the basis of their elevated transcript levels during appressorium differentiation.
27315248	8	30	dep	levels	1139:1144	arg1	the					1100:1102	the	1100:1102	the	1100:1102	We first confirmed that chitosan localizes to the germ tube and appressorium, then deleted CDA genes on the basis of their elevated transcript levels during appressorium differentiation.
27315248	11	31	theme	mutant	1576:1581	arg1	strains					1583:1589	the mutant strains	1572:1589	the mutant strains	1572:1589	Despite the lack of appressorium development on artificial surfaces, pathogenicity was unaffected in the mutant strains.
27315248	3	32	mod	modified	488:495	arg3	deacetylation					500:512	deacetylation	500:512	deacetylation	500:512	Chitin, one of the main polysaccharide components of the wall, can be chemically modified by deacetylation.
27315248	3	32	mod	modified	488:495	arg1	components					446:455	the main polysaccharide components	422:455	the main polysaccharide components of the wall	422:467	Chitin, one of the main polysaccharide components of the wall, can be chemically modified by deacetylation.
27315248	3	32	mod	modified	488:495	arg1	one					415:417	one	415:417	one	415:417	Chitin, one of the main polysaccharide components of the wall, can be chemically modified by deacetylation.
27315248	3	32	mod	modified	488:495	arg1	Chitin					407:412	Chitin	407:412	Chitin	407:412	Chitin, one of the main polysaccharide components of the wall, can be chemically modified by deacetylation.
27315248	14	33	theme	appressorium	2110:2121	arg1	development					2123:2133	appressorium development	2110:2133	appressorium development	2110:2133	This study thus reveals a novel role for chitosan in phytopathogenic fungi, and gives further insight into the mechanisms governing appressorium development in M.oryzae.
27315248	10	34	theme	germling	1423:1430	arg1	adhesion					1432:1439	germling adhesion	1423:1439	germling adhesion	1423:1439	Surprisingly, the addition of exogenous chitosan fully restored germling adhesion and appressorium development.
27315248	11	35	theme	appressorium	1491:1502	arg1	development					1504:1514	appressorium development	1491:1514	appressorium development	1491:1514	Despite the lack of appressorium development on artificial surfaces, pathogenicity was unaffected in the mutant strains.
27315248	3	36	theme	components	446:455	arg1	components					446:455	the main polysaccharide components	422:455	the main polysaccharide components of the wall	422:467	Chitin, one of the main polysaccharide components of the wall, can be chemically modified by deacetylation.
27315248	3	36	theme	components	446:455	arg1	one					415:417	one	415:417	one	415:417	Chitin, one of the main polysaccharide components of the wall, can be chemically modified by deacetylation.
27315248	3	36	theme	components	446:455	arg1	Chitin					407:412	Chitin	407:412	Chitin	407:412	Chitin, one of the main polysaccharide components of the wall, can be chemically modified by deacetylation.
27315248	0	37	theme	Germling	18:25	arg1	Adhesion					27:34	Germling Adhesion	18:34	Germling Adhesion	18:34	Chitosan Mediates Germling Adhesion in Magnaporthe oryzae and Is Required for Surface Sensing and Germling Morphogenesis.
27315248	2	38	theme	fungi	352:356	arg1	interactions					336:347	the interactions	332:347	the interactions of fungi with their physical and biological environments	332:404	The composition of the cell wall can therefore influence the interactions of fungi with their physical and biological environments.
27315248	12	39	theme	Further	1592:1598	arg1	analyses					1600:1607	Further analyses	1592:1607	Further analyses	1592:1607	Further analyses demonstrated that cuticular waxes are sufficient to over-ride the requirement for chitosan during appressorium development on the plant surface.
27315248	1	40	theme	cellular	189:196	arg1	integrity					198:206	cellular integrity	189:206	cellular integrity	189:206	The fungal cell wall not only plays a critical role in maintaining cellular integrity, but also forms the interface between fungi and their environment.
27315248	6	41	theme	full	873:876	arg1	pathogenesis					878:889	full pathogenesis	873:889	full pathogenesis	873:889	Here, it has long been hypothesized to act as a 'stealth' molecule, necessary for full pathogenesis.
27315248	9	42	from	appressoria	1311:1321	arg1	surfaces					1349:1356	artificial hydrophobic surfaces	1326:1356	artificial hydrophobic surfaces	1326:1356	Germlings of the deletion strains showed loss of chitin deacetylation, and were compromised in their ability to adhere and form appressoria on artificial hydrophobic surfaces.
27315248	3	43	theme	main	426:429	arg1	components					446:455	the main polysaccharide components	422:455	the main polysaccharide components of the wall	422:467	Chitin, one of the main polysaccharide components of the wall, can be chemically modified by deacetylation.
27315248	5	44	theme	cell	731:734	arg1	wall					736:739	the cell wall	727:739	the cell wall of infection structures in phytopathogenic fungi	727:788	Chitosan has previously been shown to accumulate in the cell wall of infection structures in phytopathogenic fungi.
27315248	10	45	theme	chitosan	1399:1406	arg1	addition					1377:1384	the addition	1373:1384	the addition of exogenous chitosan	1373:1406	Surprisingly, the addition of exogenous chitosan fully restored germling adhesion and appressorium development.
27315248	13	46	contain	have	1778:1781	arg2	role					1785:1788	a role	1783:1788	a role	1783:1788	Thus, chitosan does not have a role as a 'stealth' molecule, but instead mediates the adhesion of germlings to surfaces, thereby allowing the perception of the physical stimuli necessary to promote appressorium development.
27315248	13	46	contain	have	1778:1781	arg1	chitosan					1760:1767	chitosan	1760:1767	chitosan	1760:1767	Thus, chitosan does not have a role as a 'stealth' molecule, but instead mediates the adhesion of germlings to surfaces, thereby allowing the perception of the physical stimuli necessary to promote appressorium development.
27315248	13	46	contain	have	1778:1781	arg2	molecule					1805:1812	a 'stealth' molecule	1793:1812	a 'stealth' molecule	1793:1812	Thus, chitosan does not have a role as a 'stealth' molecule, but instead mediates the adhesion of germlings to surfaces, thereby allowing the perception of the physical stimuli necessary to promote appressorium development.
27315248	7	47	theme	crop	919:922	arg1	pathogen					924:931	crop pathogen	919:931	crop pathogen	919:931	In this study, we used the crop pathogen and model organism Magnaporthe oryzae to test this hypothesis.
27315248	2	48	theme	biological	382:391	arg1	environments					393:404	their physical and biological environments	363:404	their physical and biological environments	363:404	The composition of the cell wall can therefore influence the interactions of fungi with their physical and biological environments.
27315248	6	49	theme	necessary	859:867	arg1	molecule					849:856	a 'stealth' molecule	837:856	a 'stealth' molecule	837:856	Here, it has long been hypothesized to act as a 'stealth' molecule, necessary for full pathogenesis.
27315248	6	49	theme	necessary	859:867	arg1	it					797:798	it	797:798	it	797:798	Here, it has long been hypothesized to act as a 'stealth' molecule, necessary for full pathogenesis.
27315248	7	50	theme	organism	943:950	arg1	oryzae					964:969	the crop pathogen and model organism Magnaporthe oryzae	915:969	the crop pathogen and model organism Magnaporthe oryzae	915:969	In this study, we used the crop pathogen and model organism Magnaporthe oryzae to test this hypothesis.
27315248	14	51	from	role	2010:2013	arg1	fungi					2047:2051	phytopathogenic fungi	2031:2051	phytopathogenic fungi	2031:2051	This study thus reveals a novel role for chitosan in phytopathogenic fungi, and gives further insight into the mechanisms governing appressorium development in M.oryzae.
27315248	0	52	dep	Magnaporthe	39:49	arg1	oryzae					51:56	Magnaporthe oryzae	39:56	Magnaporthe oryzae	39:56	Chitosan Mediates Germling Adhesion in Magnaporthe oryzae and Is Required for Surface Sensing and Germling Morphogenesis.
27315248	7	53	theme	pathogen	924:931	arg1	oryzae					964:969	the crop pathogen and model organism Magnaporthe oryzae	915:969	the crop pathogen and model organism Magnaporthe oryzae	915:969	In this study, we used the crop pathogen and model organism Magnaporthe oryzae to test this hypothesis.
27315248	13	54	theme	appressorium	1952:1963	arg1	development					1965:1975	appressorium development	1952:1975	appressorium development	1952:1975	Thus, chitosan does not have a role as a 'stealth' molecule, but instead mediates the adhesion of germlings to surfaces, thereby allowing the perception of the physical stimuli necessary to promote appressorium development.
27315248	7	55	theme	Magnaporthe	952:962	arg1	oryzae					964:969	the crop pathogen and model organism Magnaporthe oryzae	915:969	the crop pathogen and model organism Magnaporthe oryzae	915:969	In this study, we used the crop pathogen and model organism Magnaporthe oryzae to test this hypothesis.
27315248	2	56	with	interactions	336:347	arg1	environments					393:404	their physical and biological environments	363:404	their physical and biological environments	363:404	The composition of the cell wall can therefore influence the interactions of fungi with their physical and biological environments.
27315248	11	57	theme	artificial	1519:1528	arg1	surfaces					1530:1537	artificial surfaces	1519:1537	artificial surfaces	1519:1537	Despite the lack of appressorium development on artificial surfaces, pathogenicity was unaffected in the mutant strains.
27315248	12	58	theme	appressorium	1707:1718	arg1	development					1720:1730	appressorium development	1707:1730	appressorium development	1707:1730	Further analyses demonstrated that cuticular waxes are sufficient to over-ride the requirement for chitosan during appressorium development on the plant surface.
27315248	1	59	theme	fungal	126:131	arg1	wall					138:141	The fungal cell wall	122:141	The fungal cell wall	122:141	The fungal cell wall not only plays a critical role in maintaining cellular integrity, but also forms the interface between fungi and their environment.
27315248	7	60	used	used	910:913	arg2	we					907:908	we	907:908	we	907:908	In this study, we used the crop pathogen and model organism Magnaporthe oryzae to test this hypothesis.
27315248	4	61	theme	chitin	574:579	arg1	CDAs					595:598	CDAs	595:598	CDAs	595:598	This reaction is catalyzed by a family of enzymes known as chitin deacetylases (CDAs), and results in the formation of chitosan, a polymer of β1,4-glucosamine.
27315248	4	61	theme	chitin	574:579	arg1	deacetylases					581:592	chitin deacetylases	574:592	chitin deacetylases (CDAs)	574:599	This reaction is catalyzed by a family of enzymes known as chitin deacetylases (CDAs), and results in the formation of chitosan, a polymer of β1,4-glucosamine.
27315248	7	62	theme	model	937:941	arg1	organism					943:950	model organism	937:950	model organism	937:950	In this study, we used the crop pathogen and model organism Magnaporthe oryzae to test this hypothesis.
27315248	13	63	theme	physical	1914:1921	arg1	stimuli					1923:1929	the physical stimuli	1910:1929	the physical stimuli necessary to promote appressorium development	1910:1975	Thus, chitosan does not have a role as a 'stealth' molecule, but instead mediates the adhesion of germlings to surfaces, thereby allowing the perception of the physical stimuli necessary to promote appressorium development.
27315248	3	64	theme	wall	464:467	arg1	components					446:455	the main polysaccharide components	422:455	the main polysaccharide components of the wall	422:467	Chitin, one of the main polysaccharide components of the wall, can be chemically modified by deacetylation.
27315248	13	65	theme	germlings	1852:1860	arg1	adhesion					1840:1847	the adhesion	1836:1847	the adhesion of germlings to surfaces	1836:1872	Thus, chitosan does not have a role as a 'stealth' molecule, but instead mediates the adhesion of germlings to surfaces, thereby allowing the perception of the physical stimuli necessary to promote appressorium development.
27315248	12	66	theme	cuticular	1627:1635	arg1	waxes					1637:1641	cuticular waxes	1627:1641	cuticular waxes	1627:1641	Further analyses demonstrated that cuticular waxes are sufficient to over-ride the requirement for chitosan during appressorium development on the plant surface.
27315248	3	67	theme	polysaccharide	431:444	arg1	components					446:455	the main polysaccharide components	422:455	the main polysaccharide components of the wall	422:467	Chitin, one of the main polysaccharide components of the wall, can be chemically modified by deacetylation.
27315248	13	68	theme	stimuli	1923:1929	arg1	perception					1896:1905	the perception	1892:1905	the perception of the physical stimuli necessary to promote appressorium development	1892:1975	Thus, chitosan does not have a role as a 'stealth' molecule, but instead mediates the adhesion of germlings to surfaces, thereby allowing the perception of the physical stimuli necessary to promote appressorium development.
27315248	4	69	theme	chitosan	634:641	arg1	formation					621:629	the formation	617:629	the formation of chitosan, a polymer of β1,4-glucosamine	617:672	This reaction is catalyzed by a family of enzymes known as chitin deacetylases (CDAs), and results in the formation of chitosan, a polymer of β1,4-glucosamine.
27315248	2	70	theme	physical	369:376	arg1	environments					393:404	their physical and biological environments	363:404	their physical and biological environments	363:404	The composition of the cell wall can therefore influence the interactions of fungi with their physical and biological environments.
27315248	4	71	theme	enzymes	557:563	arg1	family					547:552	a family	545:552	a family of enzymes known as chitin deacetylases (CDAs)	545:599	This reaction is catalyzed by a family of enzymes known as chitin deacetylases (CDAs), and results in the formation of chitosan, a polymer of β1,4-glucosamine.
27315248	5	72	from	wall	736:739	arg1	fungi					784:788	phytopathogenic fungi	768:788	phytopathogenic fungi	768:788	Chitosan has previously been shown to accumulate in the cell wall of infection structures in phytopathogenic fungi.
27315248	5	73	theme	phytopathogenic	768:782	arg1	fungi					784:788	phytopathogenic fungi	768:788	phytopathogenic fungi	768:788	Chitosan has previously been shown to accumulate in the cell wall of infection structures in phytopathogenic fungi.
27315248	13	74	theme	necessary	1931:1939	arg1	stimuli					1923:1929	the physical stimuli	1910:1929	the physical stimuli necessary to promote appressorium development	1910:1975	Thus, chitosan does not have a role as a 'stealth' molecule, but instead mediates the adhesion of germlings to surfaces, thereby allowing the perception of the physical stimuli necessary to promote appressorium development.
27315248	8	75	theme	CDA	1087:1089	arg1	genes					1091:1095	CDA genes	1087:1095	CDA genes	1087:1095	We first confirmed that chitosan localizes to the germ tube and appressorium, then deleted CDA genes on the basis of their elevated transcript levels during appressorium differentiation.
24239026	3	0	theme	developmental	587:599	arg1	characteristics					601:615	the developmental characteristics	583:615	not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling	574:678	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	4	1	theme	intermediate	929:940	arg1	water					942:946	intermediate water	929:946	intermediate water	929:946	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	3	2	theme	grain	636:640	arg1	number/ear					642:651	grain number/ear	636:651	grain number/ear	636:651	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	5	3	theme	more	1338:1341	arg1	water					1353:1357	water	1353:1357	water	1353:1357	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	5	3	theme	more	1338:1341	arg1	amount					1343:1348	more amount	1338:1348	more amount of water	1338:1357	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	4	4	theme	maturity	1003:1010	arg1	levels					962:967	increasing levels	951:967	increasing levels of nitrogen application and grain maturity	951:1010	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	7	5	theme	status	1654:1659	arg1	dependence					1621:1630	a clear nutrient and developmental stage dependence	1580:1630	a clear nutrient and developmental stage dependence of grain tissue water status in maize	1580:1668	This study strongly indicated a clear nutrient and developmental stage dependence of grain tissue water status in maize.
24239026	6	6	theme	changes	1398:1404	arg1	results					1383:1389	The results	1379:1389	The results of the changes in T2	1379:1410	The results of the changes in T2 showed that water status during grain development was not only affected by developmental processes but also by nitrogen supply to plants.
24239026	7	7	theme	tissue	1641:1646	arg1	status					1654:1659	grain tissue water status	1635:1659	grain tissue water status in maize	1635:1668	This study strongly indicated a clear nutrient and developmental stage dependence of grain tissue water status in maize.
24239026	7	8	from	dependence	1621:1630	arg1	maize					1664:1668	maize	1664:1668	maize	1664:1668	This study strongly indicated a clear nutrient and developmental stage dependence of grain tissue water status in maize.
24239026	4	9	theme	nitrogen	972:979	arg1	application					981:991	nitrogen application	972:991	nitrogen application	972:991	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	1	10	theme	developing	178:187	arg1	grains					189:194	developing grains	178:194	developing grains of maize (Zea mays L.) grown under different nitrogen levels	178:255	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	3	11	theme	developmental	491:503	arg1	characteristics					505:519	the grain developmental characteristics	481:519	the grain developmental characteristics	481:519	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	3	12	dep	characteristics	601:615	arg1	only					578:581	only	578:581	only	578:581	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	3	13	theme	grain	666:670	arg1	filling					672:678	grain filling	666:678	grain filling	666:678	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	0	14	theme	different	124:132	arg1	levels					143:148	different nitrogen levels	124:148	different nitrogen levels	124:148	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	3	15	theme	NMR	749:751	arg1	relaxation					753:762	NMR relaxation	749:762	NMR relaxation	749:762	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	4	16	theme	water	1079:1083	arg1	proportion					1065:1074	more proportion	1060:1074	more proportion of water to both bound- and intermediate states	1060:1122	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	3	17	theme	bound	700:704	arg1	water					706:710	also bound water	695:710	also bound water characterized by the T2 component of NMR relaxation	695:762	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	6	18	theme	grain	1444:1448	arg1	development					1450:1460	grain development	1444:1460	grain development	1444:1460	The results of the changes in T2 showed that water status during grain development was not only affected by developmental processes but also by nitrogen supply to plants.
24239026	7	19	theme	developmental	1601:1613	arg1	dependence					1621:1630	a clear nutrient and developmental stage dependence	1580:1630	a clear nutrient and developmental stage dependence of grain tissue water status in maize	1580:1668	This study strongly indicated a clear nutrient and developmental stage dependence of grain tissue water status in maize.
24239026	1	20	theme	resonance	296:304	arg1	spectroscopy					312:323	nuclear magnetic resonance (NMR) spectroscopy	279:323	nuclear magnetic resonance (NMR) spectroscopy	279:323	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	1	21	from	Changes	151:157	arg1	status					168:173	water status	162:173	water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels	162:255	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	3	22	theme	nitrogen	779:786	arg1	%					806:806	50-70%	801:806	50-70%	801:806	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	3	22	theme	nitrogen	779:786	arg1	application					788:798	nitrogen application	779:798	nitrogen application (50-70%)	779:807	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	5	23	theme	bound	1366:1370	arg1	state					1372:1376	the bound state	1362:1376	the bound state	1362:1376	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	0	24	theme	grains	85:90	arg1	status					64:69	water status	58:69	water status of developing grains of maize (Zea mays L.)	58:113	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	6	25	theme	nitrogen	1523:1530	arg1	supply					1532:1537	nitrogen supply	1523:1537	nitrogen supply to plants	1523:1547	The results of the changes in T2 showed that water status during grain development was not only affected by developmental processes but also by nitrogen supply to plants.
24239026	2	26	theme	N	454:454	arg1	ha					456:457	0, 120 and 180 kg N ha(-1)	436:461	0, 120 and 180 kg N ha(-1)	436:461	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	2	26	theme	N	454:454	arg1	nitrogen					426:433	nitrogen	426:433	nitrogen (0, 120 and 180 kg N ha(-1))	426:462	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	0	27	theme	maize	95:99	arg1	grains					85:90	developing grains	74:90	developing grains of maize (Zea mays L.)	74:113	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	5	28	theme	protein	1219:1225	arg1	contents					1238:1245	protein and starch contents	1219:1245	contents	1238:1245	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	2	29	theme	water	357:361	arg1	status					363:368	water status	357:368	water status of grains	357:378	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	0	30	theme	Zea	102:104	arg1	L.					111:112	Zea mays L.	102:112	Zea mays L.	102:112	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	0	30	theme	Zea	102:104	arg1	maize					95:99	maize	95:99	maize (Zea mays L.)	95:113	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	4	31	theme	responses	901:909	arg1	patterns					889:896	the patterns	885:896	the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity	885:1010	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	5	32	theme	starch	1231:1236	arg1	contents					1238:1245	protein and starch contents	1219:1245	contents	1238:1245	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	0	33	theme	Nuclear	0:6	arg1	resonance					17:25	Nuclear magnetic resonance	0:25	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.)	0:113	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	1	34	theme	different	231:239	arg1	levels					250:255	different nitrogen levels	231:255	different nitrogen levels	231:255	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	6	35	from	results	1383:1389	arg1	T2					1409:1410	T2	1409:1410	T2	1409:1410	The results of the changes in T2 showed that water status during grain development was not only affected by developmental processes but also by nitrogen supply to plants.
24239026	4	36	theme	free	914:917	arg1	water					919:923	free water	914:923	free water	914:923	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	2	37	theme	due	380:382	arg1	changes					346:352	distinct changes	337:352	distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1))	337:462	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	0	38	theme	resonance	17:25	arg1	characterisation					38:53	Nuclear magnetic resonance relaxation characterisation	0:53	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.)	0:113	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	2	39	theme	nitrogen	426:433	arg1	levels					416:421	different levels	406:421	different levels of nitrogen (0, 120 and 180 kg N ha(-1))	406:462	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	2	40	from	changes	346:352	arg1	status					363:368	water status	357:368	water status of grains	357:378	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	2	41	theme	levels	416:421	arg1	application					391:401	the application	387:401	the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1))	387:462	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	3	42	theme	properties	547:556	arg1	comparison					467:476	A comparison	465:476	A comparison of the grain developmental characteristics, composition and physical properties	465:556	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	5	43	theme	nitrogen	1269:1276	arg1	treatments					1278:1287	higher nitrogen treatments	1262:1287	higher nitrogen treatments	1262:1287	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	0	44	theme	water	58:62	arg1	status					64:69	water status	58:69	water status of developing grains of maize (Zea mays L.)	58:113	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	1	45	theme	nuclear	279:285	arg1	NMR					307:309	NMR	307:309	NMR	307:309	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	1	45	theme	nuclear	279:285	arg1	resonance					296:304	nuclear magnetic resonance	279:304	nuclear magnetic resonance (NMR) spectroscopy	279:323	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	4	46	theme	increasing	951:960	arg1	levels					962:967	increasing levels	951:967	increasing levels of nitrogen application and grain maturity	951:1010	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	4	47	theme	free	1136:1139	arg1	state					1141:1145	free state	1136:1145	free state	1136:1145	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	1	48	theme	maize	199:203	arg1	grains					189:194	developing grains	178:194	developing grains of maize (Zea mays L.) grown under different nitrogen levels	178:255	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	7	49	from	status	1654:1659	arg1	maize					1664:1668	maize	1664:1668	maize	1664:1668	This study strongly indicated a clear nutrient and developmental stage dependence of grain tissue water status in maize.
24239026	7	50	theme	nutrient	1588:1595	arg1	dependence					1621:1630	a clear nutrient and developmental stage dependence	1580:1630	a clear nutrient and developmental stage dependence of grain tissue water status in maize	1580:1668	This study strongly indicated a clear nutrient and developmental stage dependence of grain tissue water status in maize.
24239026	1	51	theme	Zea	206:208	arg1	maize					199:203	maize	199:203	maize (Zea mays L.)	199:217	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	1	51	theme	Zea	206:208	arg1	L.					215:216	Zea mays L.	206:216	Zea mays L.	206:216	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	7	52	from	maize	1664:1668	arg1	dependence					1621:1630	a clear nutrient and developmental stage dependence	1580:1630	a clear nutrient and developmental stage dependence of grain tissue water status in maize	1580:1668	This study strongly indicated a clear nutrient and developmental stage dependence of grain tissue water status in maize.
24239026	3	53	theme	grain	622:626	arg1	weight					628:633	grain weight	622:633	grain weight	622:633	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	7	54	theme	water	1648:1652	arg1	status					1654:1659	grain tissue water status	1635:1659	grain tissue water status in maize	1635:1668	This study strongly indicated a clear nutrient and developmental stage dependence of grain tissue water status in maize.
24239026	4	55	theme	nitrogen	1027:1034	arg1	application					1036:1046	nitrogen application	1027:1046	nitrogen application	1027:1046	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	1	56	theme	water	162:166	arg1	status					168:173	water status	162:173	water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels	162:255	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	7	57	theme	grain	1635:1639	arg1	status					1654:1659	grain tissue water status	1635:1659	grain tissue water status in maize	1635:1668	This study strongly indicated a clear nutrient and developmental stage dependence of grain tissue water status in maize.
24239026	4	58	theme	application	981:991	arg1	levels					962:967	increasing levels	951:967	increasing levels of nitrogen application and grain maturity	951:1010	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	4	59	theme	grain	997:1001	arg1	maturity					1003:1010	grain maturity	997:1010	grain maturity	997:1010	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	3	60	theme	grain	485:489	arg1	characteristics					505:519	the grain developmental characteristics	481:519	the grain developmental characteristics	481:519	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	1	61	theme	grains	189:194	arg1	status					168:173	water status	162:173	water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels	162:255	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	5	62	from	increases	1206:1214	arg1	contents					1238:1245	protein and starch contents	1219:1245	contents	1238:1245	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	1	63	theme	magnetic	287:294	arg1	NMR					307:309	NMR	307:309	NMR	307:309	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	1	63	theme	magnetic	287:294	arg1	resonance					296:304	nuclear magnetic resonance	279:304	nuclear magnetic resonance (NMR) spectroscopy	279:323	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	0	64	theme	nitrogen	134:141	arg1	levels					143:148	different nitrogen levels	124:148	different nitrogen levels	124:148	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	3	65	theme	filling	672:678	arg1	rate					658:661	rate	658:661	rate of grain filling	658:678	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	3	65	theme	filling	672:678	arg1	weight					628:633	grain weight	622:633	grain weight	622:633	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	3	65	theme	filling	672:678	arg1	number/ear					642:651	grain number/ear	636:651	grain number/ear	636:651	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	3	66	theme	composition	522:532	arg1	comparison					467:476	A comparison	465:476	A comparison of the grain developmental characteristics, composition and physical properties	465:556	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	3	67	theme	relaxation	753:762	arg1	component					736:744	the T2 component	729:744	the T2 component of NMR relaxation	729:762	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	0	68	theme	developing	74:83	arg1	grains					85:90	developing grains	74:90	developing grains of maize (Zea mays L.)	74:113	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	3	69	theme	characteristics	505:519	arg1	comparison					467:476	A comparison	465:476	A comparison of the grain developmental characteristics, composition and physical properties	465:556	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	7	70	theme	stage	1615:1619	arg1	dependence					1621:1630	a clear nutrient and developmental stage dependence	1580:1630	a clear nutrient and developmental stage dependence of grain tissue water status in maize	1580:1668	This study strongly indicated a clear nutrient and developmental stage dependence of grain tissue water status in maize.
24239026	4	71	theme	more	1060:1063	arg1	proportion					1065:1074	more proportion	1060:1074	more proportion of water to both bound- and intermediate states	1060:1122	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	6	72	theme	water	1424:1428	arg1	status					1430:1435	water status	1424:1435	water status during grain development	1424:1460	The results of the changes in T2 showed that water status during grain development was not only affected by developmental processes but also by nitrogen supply to plants.
24239026	3	73	theme	T2	733:734	arg1	component					736:744	the T2 component	729:744	the T2 component of NMR relaxation	729:762	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	6	74	from	T2	1409:1410	arg1	results					1383:1389	The results	1379:1389	The results of the changes in T2	1379:1410	The results of the changes in T2 showed that water status during grain development was not only affected by developmental processes but also by nitrogen supply to plants.
24239026	7	75	theme	clear	1582:1586	arg1	dependence					1621:1630	a clear nutrient and developmental stage dependence	1580:1630	a clear nutrient and developmental stage dependence of grain tissue water status in maize	1580:1668	This study strongly indicated a clear nutrient and developmental stage dependence of grain tissue water status in maize.
24239026	2	76	theme	kg	451:452	arg1	ha					456:457	0, 120 and 180 kg N ha(-1)	436:461	0, 120 and 180 kg N ha(-1)	436:461	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	2	76	theme	kg	451:452	arg1	nitrogen					426:433	nitrogen	426:433	nitrogen (0, 120 and 180 kg N ha(-1))	426:462	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	0	77	theme	mays	106:109	arg1	L.					111:112	Zea mays L.	102:112	Zea mays L.	102:112	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	0	77	theme	mays	106:109	arg1	maize					95:99	maize	95:99	maize (Zea mays L.)	95:113	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	5	78	theme	water	1353:1357	arg1	water					1353:1357	water	1353:1357	water	1353:1357	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	5	78	theme	water	1353:1357	arg1	amount					1343:1348	more amount	1338:1348	more amount of water	1338:1357	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	4	79	theme	bound-	1093:1098	arg1	states					1117:1122	both bound- and intermediate states	1088:1122	both bound- and intermediate states	1088:1122	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	0	80	theme	magnetic	8:15	arg1	resonance					17:25	Nuclear magnetic resonance	0:25	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.)	0:113	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	6	81	theme	developmental	1487:1499	arg1	processes					1501:1509	developmental processes	1487:1509	developmental processes	1487:1509	The results of the changes in T2 showed that water status during grain development was not only affected by developmental processes but also by nitrogen supply to plants.
24239026	5	82	from	contents	1238:1245	arg1	grains					1250:1255	grains	1250:1255	grains from higher nitrogen treatments	1250:1287	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	5	82	from	contents	1238:1245	arg1	treatments					1278:1287	higher nitrogen treatments	1262:1287	higher nitrogen treatments	1262:1287	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	4	83	theme	intermediate	1104:1115	arg1	states					1117:1122	both bound- and intermediate states	1088:1122	both bound- and intermediate states	1088:1122	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	2	84	theme	distinct	337:344	arg1	changes					346:352	distinct changes	337:352	distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1))	337:462	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	0	85	theme	relaxation	27:36	arg1	characterisation					38:53	Nuclear magnetic resonance relaxation characterisation	0:53	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.)	0:113	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	1	86	theme	nitrogen	241:248	arg1	levels					250:255	different nitrogen levels	231:255	different nitrogen levels	231:255	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	6	87	from	changes	1398:1404	arg1	T2					1409:1410	T2	1409:1410	T2	1409:1410	The results of the changes in T2 showed that water status during grain development was not only affected by developmental processes but also by nitrogen supply to plants.
24239026	5	88	from	treatments	1278:1287	arg1	grains					1250:1255	grains	1250:1255	grains from higher nitrogen treatments	1250:1287	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	5	88	from	treatments	1278:1287	arg1	contents					1238:1245	protein and starch contents	1219:1245	contents	1238:1245	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	5	89	theme	concomitant	1194:1204	arg1	increases					1206:1214	the concomitant increases	1190:1214	the concomitant increases in protein and starch contents in grains from higher nitrogen treatments	1190:1287	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	2	90	theme	grains	373:378	arg1	status					363:368	water status	357:368	water status of grains	357:378	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	3	91	theme	developmental	813:825	arg1	stages					827:832	developmental stages	813:832	developmental stages leading to maturation (10-60%)	813:863	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	3	92	theme	physical	538:545	arg1	properties					547:556	physical properties	538:556	physical properties	538:556	A comparison of the grain developmental characteristics, composition and physical properties indicated that, not only the developmental characteristics like grain weight, grain number/ear, and rate of grain filling increased, but also bound water characterized by the T2 component of NMR relaxation increased with nitrogen application (50-70%) and developmental stages leading to maturation (10-60%).
24239026	5	93	theme	higher	1262:1267	arg1	treatments					1278:1287	higher nitrogen treatments	1262:1287	higher nitrogen treatments	1262:1287	These changes are further corroborated by the concomitant increases in protein and starch contents in grains from higher nitrogen treatments as macromolecules like protein and starch retain more amount of water in the bound state.
24239026	0	94	theme	status	64:69	arg1	characterisation					38:53	Nuclear magnetic resonance relaxation characterisation	0:53	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.)	0:113	Nuclear magnetic resonance relaxation characterisation of water status of developing grains of maize (Zea mays L.) grown at different nitrogen levels.
24239026	2	95	theme	different	406:414	arg1	levels					416:421	different levels	406:421	different levels of nitrogen (0, 120 and 180 kg N ha(-1))	406:462	There were distinct changes in water status of grains due to the application of different levels of nitrogen (0, 120 and 180 kg N ha(-1)).
24239026	4	96	from	consistency	870:880	arg1	patterns					889:896	the patterns	885:896	the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity	885:1010	The consistency in the patterns of responses to free water and intermediate water to increasing levels of nitrogen application and grain maturity suggested that nitrogen application resulted in more proportion of water to both bound- and intermediate states and less in free state.
24239026	1	97	theme	mays	210:213	arg1	maize					199:203	maize	199:203	maize (Zea mays L.)	199:217	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24239026	1	97	theme	mays	210:213	arg1	L.					215:216	Zea mays L.	206:216	Zea mays L.	206:216	Changes in water status of developing grains of maize (Zea mays L.) grown under different nitrogen levels were characterized by nuclear magnetic resonance (NMR) spectroscopy.
24212582	7	0	theme	mapping	1229:1235	arg1	mode					1245:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	9	1	from	increase	1777:1784	arg1	area					1797:1800	contact area	1789:1800	contact area	1789:1800	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	1	2	theme	all	131:133	arg1	surfaces					157:164	virtually all natural and synthetic surfaces	121:164	virtually all natural and synthetic surfaces	121:164	Adhesion of bacteria occurs on virtually all natural and synthetic surfaces and is crucial for their survival.
24212582	4	3	theme	bacterial	565:573	arg1	forces					584:589	bacterial adhesion forces	565:589	bacterial adhesion forces	565:589	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	6	4	theme	%	1038:1038	arg1	deformation					1052:1062	a 40% ellipsoidal deformation	1034:1062	a 40% ellipsoidal deformation of the bacterial cell wall	1034:1089	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	7	5	theme	adhering	1144:1151	arg1	staphylococci					1153:1165	adhering staphylococci	1144:1165	adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode	1144:1248	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	9	6	from	wall	1755:1758	arg1	force					1830:1834	adhesion force	1821:1834	adhesion force	1821:1834	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	5	7	theme	isogenic	823:830	arg1	mutants					838:844	their more deformable isogenic Δpbp4 mutants	801:844	their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking	801:895	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	6	8	theme	Waals	939:943	arg1	forces					954:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	6	9	theme	Δpbp4	1111:1115	arg1	mutants					1117:1123	the Δpbp4 mutants	1107:1123	the Δpbp4 mutants	1107:1123	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	5	10	theme	adhesion	662:669	arg1	forces					671:676	The long-range adhesion forces	647:676	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN)	647:743	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	5	11	theme	forces	781:786	arg1	one-third					762:770	one-third	762:770	one-third	762:770	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	5	11	theme	forces	781:786	arg1	forces					781:786	these forces	775:786	these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking	775:895	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	6	12	theme	Lifshitz-Van	922:933	arg1	forces					954:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	9	13	theme	cell	1668:1671	arg1	surface					1673:1679	the bacterial cell surface	1654:1679	the bacterial cell surface	1654:1679	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	9	14	dep	neglected	1704:1712	arg1	small					1715:1719	small	1715:1719	small	1715:1719	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	6	15	theme	measured	902:909	arg1	forces					954:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	7	16	theme	nm	1333:1334	arg1	mutants					1311:1317	the Δpbp4 mutants	1301:1317	the Δpbp4 mutants of 100 to 200 nm	1301:1334	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	1	17	theme	natural	135:141	arg1	surfaces					157:164	virtually all natural and synthetic surfaces	121:164	virtually all natural and synthetic surfaces	121:164	Adhesion of bacteria occurs on virtually all natural and synthetic surfaces and is crucial for their survival.
24212582	6	18	theme	Hamaker	1001:1007	arg1	constants					1009:1017	published Hamaker constants	991:1017	published Hamaker constants	991:1017	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	8	19	theme	Δpbp4	1449:1453	arg1	mutants					1455:1461	the Δpbp4 mutants	1445:1461	the Δpbp4 mutants	1445:1461	Across naturally occurring bacterial strains, long-range forces do not vary to the extent observed here for the Δpbp4 mutants.
24212582	4	20	theme	AFM	642:644	arg1	use					635:637	the use	631:637	the use of AFM	631:644	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	7	21	theme	property	1220:1227	arg1	mode					1245:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	1	22	theme	synthetic	147:155	arg1	surfaces					157:164	virtually all natural and synthetic surfaces	121:164	virtually all natural and synthetic surfaces	121:164	Adhesion of bacteria occurs on virtually all natural and synthetic surfaces and is crucial for their survival.
24212582	5	23	theme	aureus	706:711	arg1	nN					741:742	0.5 and 0.8 nN	729:742	0.5 and 0.8 nN	729:742	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	5	23	theme	aureus	706:711	arg1	strains					720:726	wild-type Staphylococcus aureus parent strains	681:726	wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN)	681:743	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	7	24	theme	force-quantitative	1186:1203	arg1	mode					1245:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	7	25	theme	AFM	1177:1179	arg1	mode					1245:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	3	26	theme	Bacterial	327:335	arg1	adhesion					337:344	Bacterial adhesion	327:344	Bacterial adhesion to surfaces	327:356	Bacterial adhesion to surfaces is mediated by a combination of different short- and long-range forces.
24212582	4	27	theme	new	448:450	arg1	AFM					477:479	AFM	477:479	AFM	477:479	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	4	27	theme	new	448:450	arg1	microscopy					465:474	new atomic force microscopy	448:474	new atomic force microscopy (AFM)	448:480	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	3	28	theme	forces	422:427	arg1	combination					375:385	a combination	373:385	a combination of different short- and long-range forces	373:427	Bacterial adhesion to surfaces is mediated by a combination of different short- and long-range forces.
24212582	5	29	theme	parent	713:718	arg1	nN					741:742	0.5 and 0.8 nN	729:742	0.5 and 0.8 nN	729:742	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	5	29	theme	parent	713:718	arg1	strains					720:726	wild-type Staphylococcus aureus parent strains	681:726	wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN)	681:743	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	9	30	theme	study	1525:1529	arg1	results					1509:1515	the results	1505:1515	the results of this study	1505:1529	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	6	31	theme	ellipsoidal	1040:1050	arg1	deformation					1052:1062	a 40% ellipsoidal deformation	1034:1062	a 40% ellipsoidal deformation of the bacterial cell wall	1034:1089	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	9	32	theme	cell	1750:1753	arg1	wall					1755:1758	the bacterial cell wall	1736:1758	the bacterial cell wall	1736:1758	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	4	33	theme	force	459:463	arg1	AFM					477:479	AFM	477:479	AFM	477:479	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	4	33	theme	force	459:463	arg1	microscopy					465:474	new atomic force microscopy	448:474	new atomic force microscopy (AFM)	448:480	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	5	34	theme	strains	720:726	arg1	forces					671:676	The long-range adhesion forces	647:676	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN)	647:743	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	0	35	theme	Nanoscale	0:8	arg1	deformation					20:30	Nanoscale cell wall deformation	0:30	Nanoscale cell wall deformation	0:30	Nanoscale cell wall deformation impacts long-range bacterial adhesion forces on surfaces.
24212582	5	36	from	deficient	856:864	arg1	cross-linking					883:895	peptidoglycan cross-linking	869:895	peptidoglycan cross-linking	869:895	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	6	37	theme	40	1036:1037	arg1	%					1038:1038	%	1038:1038	%	1038:1038	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	4	38	theme	bacterial	516:524	arg1	forces					535:540	long-range bacterial adhesion forces	505:540	long-range bacterial adhesion forces	505:540	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	8	39	theme	bacterial	1364:1372	arg1	strains					1374:1380	naturally occurring bacterial strains	1344:1380	naturally occurring bacterial strains	1344:1380	Across naturally occurring bacterial strains, long-range forces do not vary to the extent observed here for the Δpbp4 mutants.
24212582	0	40	theme	wall	15:18	arg1	deformation					20:30	Nanoscale cell wall deformation	0:30	Nanoscale cell wall deformation	0:30	Nanoscale cell wall deformation impacts long-range bacterial adhesion forces on surfaces.
24212582	3	41	theme	different	390:398	arg1	forces					422:427	different short- and long-range forces	390:427	different short- and long-range forces	390:427	Bacterial adhesion to surfaces is mediated by a combination of different short- and long-range forces.
24212582	9	42	from	deformation	1721:1731	arg1	force					1830:1834	adhesion force	1821:1834	adhesion force	1821:1834	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	0	43	theme	bacterial	51:59	arg1	forces					70:75	long-range bacterial adhesion forces	40:75	long-range bacterial adhesion forces	40:75	Nanoscale cell wall deformation impacts long-range bacterial adhesion forces on surfaces.
24212582	9	44	theme	bacterial	1568:1576	arg1	forces					1587:1592	long-range bacterial adhesion forces	1557:1592	long-range bacterial adhesion forces	1557:1592	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	2	45	theme	environmental	304:316	arg1	attacks					318:324	environmental attacks	304:324	environmental attacks	304:324	Once they are adhering, bacteria start growing and form a biofilm, in which they are protected against environmental attacks.
24212582	6	46	theme	wall	1086:1089	arg1	deformation					1052:1062	a 40% ellipsoidal deformation	1034:1062	a 40% ellipsoidal deformation of the bacterial cell wall	1034:1089	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	4	47	theme	adhesion	575:582	arg1	forces					584:589	bacterial adhesion forces	565:589	bacterial adhesion forces	565:589	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	9	48	dep	composition	1625:1635	arg1	the					1621:1623	the	1621:1623	the	1621:1623	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	6	49	theme	bacterial	1071:1079	arg1	wall					1086:1089	the bacterial cell wall	1067:1089	the bacterial cell wall	1067:1089	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	7	50	theme	imaging	1237:1243	arg1	mode					1245:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	9	51	theme	adhesion	1821:1828	arg1	force					1830:1834	adhesion force	1821:1834	adhesion force	1821:1834	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	9	52	from	force	1830:1834	arg1	deformation					1721:1731	a hitherto neglected, small deformation	1693:1731	a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force	1693:1834	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	5	53	theme	deformable	812:821	arg1	mutants					838:844	their more deformable isogenic Δpbp4 mutants	801:844	their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking	801:895	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	6	54	theme	adhesion	945:952	arg1	forces					954:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	7	55	from	reduction	1269:1277	arg1	mutants					1311:1317	the Δpbp4 mutants	1301:1317	the Δpbp4 mutants of 100 to 200 nm	1301:1334	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	9	56	from	therewith	1807:1815	arg1	area					1797:1800	contact area	1789:1800	contact area	1789:1800	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	5	57	theme	long-range	651:660	arg1	forces					671:676	The long-range adhesion forces	647:676	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN)	647:743	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	4	58	attach	derive	498:503	arg2	method					488:493	a new atomic force microscopy (AFM)-based method	446:493	a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM	446:644	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	4	58	attach	derive	498:503	arg1	dependence					551:560	the dependence	547:560	the dependence of bacterial adhesion forces on the loading force	547:610	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	6	59	theme	der	935:937	arg1	forces					954:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	9	60	theme	bacterial	1658:1666	arg1	surface					1673:1679	the bacterial cell surface	1654:1679	the bacterial cell surface	1654:1679	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	4	61	theme	loading	598:604	arg1	force					606:610	the loading force	594:610	the loading force	594:610	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	6	62	theme	long-range	911:920	arg1	forces					954:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces	898:959	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	9	63	theme	surface	1673:1679	arg1	composition					1625:1635	composition	1625:1635	composition	1625:1635	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	5	64	theme	peptidoglycan	869:881	arg1	cross-linking					883:895	peptidoglycan cross-linking	869:895	peptidoglycan cross-linking	869:895	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	7	65	theme	nanomechanical	1205:1218	arg1	mode					1245:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	5	66	theme	Δpbp4	832:836	arg1	mutants					838:844	their more deformable isogenic Δpbp4 mutants	801:844	their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking	801:895	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	6	67	theme	published	991:999	arg1	constants					1009:1017	published Hamaker constants	991:1017	published Hamaker constants	991:1017	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
24212582	9	68	theme	neglected	1704:1712	arg1	deformation					1721:1731	a hitherto neglected, small deformation	1693:1731	a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force	1693:1834	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	7	69	theme	peak	1181:1184	arg1	mode					1245:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	the AFM peak force-quantitative nanomechanical property mapping imaging mode	1173:1248	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	4	70	theme	-based	481:486	arg1	method					488:493	a new atomic force microscopy (AFM)-based method	446:493	a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM	446:644	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	5	71	theme	wild-type	681:689	arg1	nN					741:742	0.5 and 0.8 nN	729:742	0.5 and 0.8 nN	729:742	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	5	71	theme	wild-type	681:689	arg1	strains					720:726	wild-type Staphylococcus aureus parent strains	681:726	wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN)	681:743	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	4	72	theme	atomic	452:457	arg1	AFM					477:479	AFM	477:479	AFM	477:479	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	4	72	theme	atomic	452:457	arg1	microscopy					465:474	new atomic force microscopy	448:474	new atomic force microscopy (AFM)	448:480	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	7	73	theme	height	1262:1267	arg1	reduction					1269:1277	a height reduction	1260:1277	a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm	1260:1334	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	4	74	from	dependence	551:560	arg1	force					606:610	the loading force	594:610	the loading force	594:610	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	7	75	dep	200	1329:1331	arg1	to					1326:1327	to	1326:1327	to	1326:1327	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	9	76	theme	bacterial	1740:1748	arg1	wall					1755:1758	the bacterial cell wall	1736:1758	the bacterial cell wall	1736:1758	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	7	77	theme	staphylococci	1153:1165	arg1	imaging					1133:1139	Direct imaging	1126:1139	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode	1126:1248	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	0	78	theme	cell	10:13	arg1	deformation					20:30	Nanoscale cell wall deformation	0:30	Nanoscale cell wall deformation	0:30	Nanoscale cell wall deformation impacts long-range bacterial adhesion forces on surfaces.
24212582	5	79	from	cross-linking	883:895	arg1	deficient					856:864	deficient	856:864	deficient	856:864	The long-range adhesion forces of wild-type Staphylococcus aureus parent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4 mutants that were deficient in peptidoglycan cross-linking.
24212582	7	80	theme	Δpbp4	1305:1309	arg1	mutants					1311:1317	the Δpbp4 mutants	1301:1317	the Δpbp4 mutants of 100 to 200 nm	1301:1334	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	9	81	theme	wall	1755:1758	arg1	deformation					1721:1731	a hitherto neglected, small deformation	1693:1731	a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force	1693:1834	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	4	82	theme	adhesion	526:533	arg1	forces					535:540	long-range bacterial adhesion forces	505:540	long-range bacterial adhesion forces	505:540	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	3	83	theme	long-range	411:420	arg1	forces					422:427	different short- and long-range forces	390:427	different short- and long-range forces	390:427	Bacterial adhesion to surfaces is mediated by a combination of different short- and long-range forces.
24212582	7	84	theme	Direct	1126:1131	arg1	imaging					1133:1139	Direct imaging	1126:1139	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode	1126:1248	Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4 mutants of 100 to 200 nm.
24212582	1	85	theme	bacteria	102:109	arg1	Adhesion					90:97	Adhesion	90:97	Adhesion of bacteria	90:109	Adhesion of bacteria occurs on virtually all natural and synthetic surfaces and is crucial for their survival.
24212582	8	86	theme	occurring	1354:1362	arg1	strains					1374:1380	naturally occurring bacterial strains	1344:1380	naturally occurring bacterial strains	1344:1380	Across naturally occurring bacterial strains, long-range forces do not vary to the extent observed here for the Δpbp4 mutants.
24212582	0	87	theme	long-range	40:49	arg1	forces					70:75	long-range bacterial adhesion forces	40:75	long-range bacterial adhesion forces	40:75	Nanoscale cell wall deformation impacts long-range bacterial adhesion forces on surfaces.
24212582	3	88	theme	short-	400:405	arg1	forces					422:427	different short- and long-range forces	390:427	different short- and long-range forces	390:427	Bacterial adhesion to surfaces is mediated by a combination of different short- and long-range forces.
24212582	0	89	theme	adhesion	61:68	arg1	forces					70:75	long-range bacterial adhesion forces	40:75	long-range bacterial adhesion forces	40:75	Nanoscale cell wall deformation impacts long-range bacterial adhesion forces on surfaces.
24212582	9	90	theme	long-range	1557:1566	arg1	forces					1587:1592	long-range bacterial adhesion forces	1557:1592	long-range bacterial adhesion forces	1557:1592	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	9	91	theme	contact	1789:1795	arg1	area					1797:1800	contact area	1789:1800	contact area	1789:1800	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	4	92	theme	long-range	505:514	arg1	forces					535:540	long-range bacterial adhesion forces	505:540	long-range bacterial adhesion forces	505:540	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	8	93	theme	long-range	1383:1392	arg1	forces					1394:1399	long-range forces	1383:1399	long-range forces	1383:1399	Across naturally occurring bacterial strains, long-range forces do not vary to the extent observed here for the Δpbp4 mutants.
24212582	9	94	theme	adhesion	1578:1585	arg1	forces					1587:1592	long-range bacterial adhesion forces	1557:1592	long-range bacterial adhesion forces	1557:1592	Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.
24212582	4	95	theme	forces	584:589	arg1	dependence					551:560	the dependence	547:560	the dependence of bacterial adhesion forces on the loading force	547:610	Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM.
24212582	6	96	theme	cell	1081:1084	arg1	wall					1086:1089	the bacterial cell wall	1067:1089	the bacterial cell wall	1067:1089	The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4 mutants.
25353612	2	0	theme	melting	439:445	arg1	point					447:451	their melting point	433:451	their melting point	433:451	In this process, the carboxylic acids do not only act as grafting agent, but also as solvent media above their melting point.
25353612	6	1	theme	nanocrystals	931:942	arg1	properties					909:918	the surface properties	897:918	the surface properties of grafted nanocrystals (CNC-g-CA)	897:953	The ability to tune the surface properties of grafted nanocrystals (CNC-g-CA) was evaluated by X-ray photoelectron spectroscopy analysis.
25353612	7	2	theme	functionalized	1050:1063	arg1	CNC					1065:1067	the functionalized CNC	1046:1067	the functionalized CNC	1046:1067	The hydrophobicity behavior of the functionalized CNC was studied through the water contact-angle measurements and vapor adsorption.
25353612	0	3	with	functionalization	27:43	arg1	acids					78:82	carboxylic acids	67:82	carboxylic acids	67:82	Green process for chemical functionalization of nanocellulose with carboxylic acids.
25353612	5	4	theme	infrared	839:846	arg1	spectroscopy					848:859	infrared spectroscopy	839:859	infrared spectroscopy	839:859	The presence of the grafted carboxylic was characterized by analysis of the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR.
25353612	7	5	theme	CNC	1065:1067	arg1	behavior					1034:1041	The hydrophobicity behavior	1015:1041	The hydrophobicity behavior of the functionalized CNC	1015:1067	The hydrophobicity behavior of the functionalized CNC was studied through the water contact-angle measurements and vapor adsorption.
25353612	1	6	dep	friendly	104:111	arg1	An					85:86	An	85:86	An	85:86	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	4	7	from	change	648:653	arg1	dimensions					666:675	the CNC dimensions	658:675	the CNC dimensions	658:675	Atomic force microscopy and X-ray diffraction analyses showed no significant change in the CNC dimensions and crystallinity index after this green process.
25353612	4	7	from	change	648:653	arg1	index					695:699	crystallinity index	681:699	crystallinity index	681:699	Atomic force microscopy and X-ray diffraction analyses showed no significant change in the CNC dimensions and crystallinity index after this green process.
25353612	3	8	dep	in	465:466	arg1	situ					468:471	situ	468:471	situ	468:471	Key is the in situ solvent exchange by water evaporation driving the esterification reaction without drying the CNC.
25353612	1	9	theme	nontoxic	254:261	arg1	CA					281:282	CA	281:282	CA	281:282	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	1	9	theme	nontoxic	254:261	arg1	acids					274:278	carboxylic acids	263:278	two nontoxic carboxylic acids (CA)	250:283	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	1	9	theme	nontoxic	254:261	arg1	acid					299:302	phenylacetic acid	286:302	phenylacetic acid	286:302	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	1	9	theme	nontoxic	254:261	arg1	acid					322:325	hydrocinnamic acid	308:325	hydrocinnamic acid	308:325	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	8	10	theme	polymer	1316:1322	arg1	matrices					1324:1331	hydrophobic polymer matrices	1304:1331	hydrophobic polymer matrices	1304:1331	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	1	11	theme	carboxylic	263:272	arg1	CA					281:282	CA	281:282	CA	281:282	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	1	11	theme	carboxylic	263:272	arg1	acids					274:278	carboxylic acids	263:278	two nontoxic carboxylic acids (CA)	250:283	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	1	11	theme	carboxylic	263:272	arg1	acid					299:302	phenylacetic acid	286:302	phenylacetic acid	286:302	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	1	11	theme	carboxylic	263:272	arg1	acid					322:325	hydrocinnamic acid	308:325	hydrocinnamic acid	308:325	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	7	12	theme	vapor	1130:1134	arg1	adsorption					1136:1145	vapor adsorption	1130:1145	vapor adsorption	1130:1145	The hydrophobicity behavior of the functionalized CNC was studied through the water contact-angle measurements and vapor adsorption.
25353612	7	13	theme	hydrophobicity	1019:1032	arg1	behavior					1034:1041	The hydrophobicity behavior	1015:1041	The hydrophobicity behavior of the functionalized CNC	1015:1067	The hydrophobicity behavior of the functionalized CNC was studied through the water contact-angle measurements and vapor adsorption.
25353612	4	14	theme	force	578:582	arg1	microscopy					584:593	Atomic force microscopy	571:593	Atomic force microscopy	571:593	Atomic force microscopy and X-ray diffraction analyses showed no significant change in the CNC dimensions and crystallinity index after this green process.
25353612	6	15	theme	photoelectron	978:990	arg1	analysis					1005:1012	X-ray photoelectron spectroscopy analysis	972:1012	X-ray photoelectron spectroscopy analysis	972:1012	The ability to tune the surface properties of grafted nanocrystals (CNC-g-CA) was evaluated by X-ray photoelectron spectroscopy analysis.
25353612	5	16	theme	"	808:808	arg1	CNC					810:812	the "bulk" CNC	799:812	the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR	799:874	The presence of the grafted carboxylic was characterized by analysis of the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR.
25353612	5	17	theme	carboxylic	755:764	arg1	presence					731:738	The presence	727:738	The presence of the grafted carboxylic	727:764	The presence of the grafted carboxylic was characterized by analysis of the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR.
25353612	8	18	theme	as	1337:1338	arg1	nanoadsorbers					1340:1352	as nanoadsorbers	1337:1352	as nanoadsorbers	1337:1352	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	3	19	theme	solvent	473:479	arg1	exchange					481:488	the in situ solvent exchange	461:488	the in situ solvent exchange by water evaporation driving the esterification reaction without drying the CNC	461:568	Key is the in situ solvent exchange by water evaporation driving the esterification reaction without drying the CNC.
25353612	3	19	theme	solvent	473:479	arg1	Key					454:456	Key	454:456	Key	454:456	Key is the in situ solvent exchange by water evaporation driving the esterification reaction without drying the CNC.
25353612	4	20	theme	diffraction	605:615	arg1	analyses					617:624	Atomic force microscopy and X-ray diffraction analyses	571:624	Atomic force microscopy and X-ray diffraction analyses	571:624	Atomic force microscopy and X-ray diffraction analyses showed no significant change in the CNC dimensions and crystallinity index after this green process.
25353612	1	21	theme	environmentally	88:102	arg1	friendly					104:111	environmentally friendly	88:111	environmentally friendly	88:111	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	1	22	theme	solvent-free	173:184	arg1	esterification					186:199	a solvent-free esterification	171:199	a solvent-free esterification of cellulose nanocrystals (CNC) surface	171:239	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	5	23	theme	C	870:870	arg1	NMR					872:874	(13)C NMR	866:874	(13)C NMR	866:874	The presence of the grafted carboxylic was characterized by analysis of the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR.
25353612	8	24	theme	composite	1222:1230	arg1	manufacturing					1232:1244	composite manufacturing	1222:1244	composite manufacturing	1222:1244	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	2	25	theme	carboxylic	349:358	arg1	media					421:425	solvent media	413:425	solvent media above their melting point	413:451	In this process, the carboxylic acids do not only act as grafting agent, but also as solvent media above their melting point.
25353612	2	25	theme	carboxylic	349:358	arg1	acids					360:364	the carboxylic acids	345:364	the carboxylic acids	345:364	In this process, the carboxylic acids do not only act as grafting agent, but also as solvent media above their melting point.
25353612	0	26	theme	chemical	18:25	arg1	functionalization					27:43	chemical functionalization	18:43	chemical functionalization of nanocellulose with carboxylic acids	18:82	Green process for chemical functionalization of nanocellulose with carboxylic acids.
25353612	6	27	theme	spectroscopy	992:1003	arg1	analysis					1005:1012	X-ray photoelectron spectroscopy analysis	972:1012	X-ray photoelectron spectroscopy analysis	972:1012	The ability to tune the surface properties of grafted nanocrystals (CNC-g-CA) was evaluated by X-ray photoelectron spectroscopy analysis.
25353612	8	28	from	dispersibility	1286:1299	arg1	matrices					1324:1331	hydrophobic polymer matrices	1304:1331	hydrophobic polymer matrices	1304:1331	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	8	28	from	dispersibility	1286:1299	arg1	nanoadsorbers					1340:1352	as nanoadsorbers	1337:1352	as nanoadsorbers	1337:1352	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	3	29	theme	water	493:497	arg1	evaporation					499:509	water evaporation	493:509	water evaporation driving the esterification reaction without drying the CNC	493:568	Key is the in situ solvent exchange by water evaporation driving the esterification reaction without drying the CNC.
25353612	2	30	theme	grafting	385:392	arg1	agent					394:398	grafting agent	385:398	grafting agent	385:398	In this process, the carboxylic acids do not only act as grafting agent, but also as solvent media above their melting point.
25353612	7	31	theme	contact-angle	1099:1111	arg1	measurements					1113:1124	the water contact-angle measurements	1089:1124	the water contact-angle measurements	1089:1124	The hydrophobicity behavior of the functionalized CNC was studied through the water contact-angle measurements and vapor adsorption.
25353612	1	32	theme	simple	117:122	arg1	method					124:129	simple method	117:129	simple method	117:129	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	1	33	theme	cellulose	204:212	arg1	CNC					228:230	CNC	228:230	CNC	228:230	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	1	33	theme	cellulose	204:212	arg1	nanocrystals					214:225	cellulose nanocrystals	204:225	cellulose nanocrystals (CNC) surface	204:239	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	8	34	theme	hydrophobic	1304:1314	arg1	matrices					1324:1331	hydrophobic polymer matrices	1304:1331	hydrophobic polymer matrices	1304:1331	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	4	35	theme	X-ray	599:603	arg1	diffraction					605:615	X-ray diffraction	599:615	X-ray diffraction	599:615	Atomic force microscopy and X-ray diffraction analyses showed no significant change in the CNC dimensions and crystallinity index after this green process.
25353612	8	36	theme	phenolic	1377:1384	arg1	groups					1386:1391	phenolic groups	1377:1391	phenolic groups attached on the surface	1377:1415	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	3	37	dep	drying	555:560	arg1	CNC					566:568	the CNC	562:568	drying the CNC	555:568	Key is the in situ solvent exchange by water evaporation driving the esterification reaction without drying the CNC.
25353612	1	38	theme	nanocrystals	214:225	arg1	surface					233:239	cellulose nanocrystals (CNC) surface	204:239	cellulose nanocrystals (CNC) surface	204:239	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	4	39	theme	Atomic	571:576	arg1	microscopy					584:593	Atomic force microscopy	571:593	Atomic force microscopy	571:593	Atomic force microscopy and X-ray diffraction analyses showed no significant change in the CNC dimensions and crystallinity index after this green process.
25353612	5	40	theme	bulk	804:807	arg1	CNC					810:812	the "bulk" CNC	799:812	the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR	799:874	The presence of the grafted carboxylic was characterized by analysis of the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR.
25353612	2	41	theme	solvent	413:419	arg1	media					421:425	solvent media	413:425	solvent media above their melting point	413:451	In this process, the carboxylic acids do not only act as grafting agent, but also as solvent media above their melting point.
25353612	2	41	theme	solvent	413:419	arg1	acids					360:364	the carboxylic acids	345:364	the carboxylic acids	345:364	In this process, the carboxylic acids do not only act as grafting agent, but also as solvent media above their melting point.
25353612	1	42	theme	phenylacetic	286:297	arg1	acids					274:278	carboxylic acids	263:278	two nontoxic carboxylic acids (CA)	250:283	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	1	42	theme	phenylacetic	286:297	arg1	acid					299:302	phenylacetic acid	286:302	phenylacetic acid	286:302	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	8	43	contain	have	1273:1276	arg1	nanoparticles					1259:1271	these nanoparticles	1253:1271	these nanoparticles	1253:1271	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	8	43	contain	have	1273:1276	arg2	dispersibility					1286:1299	limited dispersibility	1278:1299	limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers	1278:1352	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	3	44	theme	esterification	523:536	arg1	reaction					538:545	the esterification reaction	519:545	the esterification reaction without drying the CNC	519:568	Key is the in situ solvent exchange by water evaporation driving the esterification reaction without drying the CNC.
25353612	0	45	theme	nanocellulose	48:60	arg1	functionalization					27:43	chemical functionalization	18:43	chemical functionalization of nanocellulose with carboxylic acids	18:82	Green process for chemical functionalization of nanocellulose with carboxylic acids.
25353612	8	46	theme	groups	1386:1391	arg1	presence					1365:1372	the presence	1361:1372	the presence of phenolic groups attached on the surface	1361:1415	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	4	47	theme	CNC	662:664	arg1	dimensions					666:675	the CNC dimensions	658:675	the CNC dimensions	658:675	Atomic force microscopy and X-ray diffraction analyses showed no significant change in the CNC dimensions and crystallinity index after this green process.
25353612	7	48	theme	water	1093:1097	arg1	measurements					1113:1124	the water contact-angle measurements	1089:1124	the water contact-angle measurements	1089:1124	The hydrophobicity behavior of the functionalized CNC was studied through the water contact-angle measurements and vapor adsorption.
25353612	5	49	theme	elemental	819:827	arg1	analysis					829:836	elemental analysis	819:836	elemental analysis	819:836	The presence of the grafted carboxylic was characterized by analysis of the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR.
25353612	4	50	theme	crystallinity	681:693	arg1	index					695:699	crystallinity index	681:699	crystallinity index	681:699	Atomic force microscopy and X-ray diffraction analyses showed no significant change in the CNC dimensions and crystallinity index after this green process.
25353612	4	51	theme	green	712:716	arg1	process					718:724	this green process	707:724	this green process	707:724	Atomic force microscopy and X-ray diffraction analyses showed no significant change in the CNC dimensions and crystallinity index after this green process.
25353612	6	52	theme	surface	901:907	arg1	properties					909:918	the surface properties	897:918	the surface properties of grafted nanocrystals (CNC-g-CA)	897:953	The ability to tune the surface properties of grafted nanocrystals (CNC-g-CA) was evaluated by X-ray photoelectron spectroscopy analysis.
25353612	5	53	with	CNC	810:812	arg1	NMR					872:874	(13)C NMR	866:874	(13)C NMR	866:874	The presence of the grafted carboxylic was characterized by analysis of the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR.
25353612	5	53	with	CNC	810:812	arg1	analysis					829:836	elemental analysis	819:836	elemental analysis	819:836	The presence of the grafted carboxylic was characterized by analysis of the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR.
25353612	5	53	with	CNC	810:812	arg1	spectroscopy					848:859	infrared spectroscopy	839:859	infrared spectroscopy	839:859	The presence of the grafted carboxylic was characterized by analysis of the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR.
25353612	6	54	theme	X-ray	972:976	arg1	analysis					1005:1012	X-ray photoelectron spectroscopy analysis	972:1012	X-ray photoelectron spectroscopy analysis	972:1012	The ability to tune the surface properties of grafted nanocrystals (CNC-g-CA) was evaluated by X-ray photoelectron spectroscopy analysis.
25353612	4	55	theme	significant	636:646	arg1	change					648:653	no significant change	633:653	no significant change in the CNC dimensions and crystallinity index	633:699	Atomic force microscopy and X-ray diffraction analyses showed no significant change in the CNC dimensions and crystallinity index after this green process.
25353612	8	56	theme	bionanoparticles	1179:1194	arg1	functionalization					1152:1168	The functionalization	1148:1168	The functionalization of these bionanoparticles	1148:1194	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	8	57	theme	limited	1278:1284	arg1	dispersibility					1286:1299	limited dispersibility	1278:1299	limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers	1278:1352	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	4	58	theme	microscopy	584:593	arg1	analyses					617:624	Atomic force microscopy and X-ray diffraction analyses	571:624	Atomic force microscopy and X-ray diffraction analyses	571:624	Atomic force microscopy and X-ray diffraction analyses showed no significant change in the CNC dimensions and crystallinity index after this green process.
25353612	5	59	theme	CNC	810:812	arg1	analysis					787:794	analysis	787:794	analysis of the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR	787:874	The presence of the grafted carboxylic was characterized by analysis of the "bulk" CNC with elemental analysis, infrared spectroscopy, and (13)C NMR.
25353612	1	60	theme	surface	233:239	arg1	esterification					186:199	a solvent-free esterification	171:199	a solvent-free esterification of cellulose nanocrystals (CNC) surface	171:239	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	0	61	theme	carboxylic	67:76	arg1	acids					78:82	carboxylic acids	67:82	carboxylic acids	67:82	Green process for chemical functionalization of nanocellulose with carboxylic acids.
25353612	1	62	theme	hydrocinnamic	308:320	arg1	acids					274:278	carboxylic acids	263:278	two nontoxic carboxylic acids (CA)	250:283	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	1	62	theme	hydrocinnamic	308:320	arg1	acid					322:325	hydrocinnamic acid	308:325	hydrocinnamic acid	308:325	An environmentally friendly and simple method, named SolReact, has been developed for a solvent-free esterification of cellulose nanocrystals (CNC) surface by using two nontoxic carboxylic acids (CA), phenylacetic acid and hydrocinnamic acid.
25353612	8	63	attach	attached	1393:1400	arg3	surface					1409:1415	the surface	1405:1415	the surface	1405:1415	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	8	63	attach	attached	1393:1400	arg2	groups					1386:1391	phenolic groups	1377:1391	phenolic groups attached on the surface	1377:1415	The functionalization of these bionanoparticles may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices and as nanoadsorbers due to the presence of phenolic groups attached on the surface.
25353612	3	64	theme	in	465:466	arg1	exchange					481:488	the in situ solvent exchange	461:488	the in situ solvent exchange by water evaporation driving the esterification reaction without drying the CNC	461:568	Key is the in situ solvent exchange by water evaporation driving the esterification reaction without drying the CNC.
25353612	3	64	theme	in	465:466	arg1	Key					454:456	Key	454:456	Key	454:456	Key is the in situ solvent exchange by water evaporation driving the esterification reaction without drying the CNC.
25353612	6	65	theme	grafted	923:929	arg1	nanocrystals					931:942	grafted nanocrystals	923:942	grafted nanocrystals (CNC-g-CA)	923:953	The ability to tune the surface properties of grafted nanocrystals (CNC-g-CA) was evaluated by X-ray photoelectron spectroscopy analysis.
25353612	6	65	theme	grafted	923:929	arg1	CNC-g-CA					945:952	CNC-g-CA	945:952	CNC-g-CA	945:952	The ability to tune the surface properties of grafted nanocrystals (CNC-g-CA) was evaluated by X-ray photoelectron spectroscopy analysis.
24861954	1	0	theme	palladium	146:154	arg1	nanoparticles					156:168	palladium nanoparticles	146:168	palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C)	146:239	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	1	1	theme	@	235:235	arg1	N-C					236:238	Pd/TiO2@N-C	228:238	Pd/TiO2@N-C	228:238	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	1	1	theme	@	235:235	arg1	carbon					220:225	nitrogen-modified porous carbon	195:225	nitrogen-modified porous carbon (Pd/TiO2@N-C)	195:239	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	5	2	theme	synergistic	821:831	arg1	effect					833:838	a synergistic effect	819:838	a synergistic effect between the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites	819:966	This activity can be attributed to a synergistic effect between the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites.
24861954	2	3	theme	sorption	316:323	arg1	isotherms					325:333	N2 sorption isotherms	313:333	N2 sorption isotherms	313:333	N2 sorption isotherms show that the catalyst has a high BET surface area (229 m(2) g(-1)) and large porosity.
24861954	3	4	with	species	489:495	arg1	states					521:526	different chemical states	502:526	different chemical states	502:526	XPS and TEM characterization of the catalyst shows that palladium species with different chemical states are well dispersed across the TiO2 and nitrogen-modified porous carbon, respectively.
24861954	0	5	with	catalyst	74:81	arg1	types					92:96	two types	88:96	two types of active sites	88:112	Hydrogenation of biofuels with formic acid over a palladium-based ternary catalyst with two types of active sites.
24861954	5	6	dep	Pd/TiO2	852:858	arg1	catalyst					863:870	a catalyst	861:870	a catalyst for dehydrogenation of formic acid	861:905	This activity can be attributed to a synergistic effect between the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites.
24861954	4	7	theme	excellent	664:672	arg1	stability					674:682	excellent stability	664:682	excellent stability towards hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source	664:781	The Pd/TiO2@N-C catalyst is very active and shows excellent stability towards hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source.
24861954	4	8	theme	Pd/TiO2	618:624	arg1	catalyst					630:637	The Pd/TiO2@N-C catalyst	614:637	The Pd/TiO2@N-C catalyst	614:637	The Pd/TiO2@N-C catalyst is very active and shows excellent stability towards hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source.
24861954	4	8	theme	Pd/TiO2	618:624	arg1	active					647:652	active	647:652	active	647:652	The Pd/TiO2@N-C catalyst is very active and shows excellent stability towards hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source.
24861954	0	9	theme	active	101:106	arg1	sites					108:112	active sites	101:112	active sites	101:112	Hydrogenation of biofuels with formic acid over a palladium-based ternary catalyst with two types of active sites.
24861954	4	10	theme	N-C	626:628	arg1	catalyst					630:637	The Pd/TiO2@N-C catalyst	614:637	The Pd/TiO2@N-C catalyst	614:637	The Pd/TiO2@N-C catalyst is very active and shows excellent stability towards hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source.
24861954	4	10	theme	N-C	626:628	arg1	active					647:652	active	647:652	active	647:652	The Pd/TiO2@N-C catalyst is very active and shows excellent stability towards hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source.
24861954	5	11	theme	acid	902:905	arg1	dehydrogenation					876:890	dehydrogenation	876:890	dehydrogenation of formic acid	876:905	This activity can be attributed to a synergistic effect between the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites.
24861954	4	12	theme	hydrogen	767:774	arg1	source					776:781	hydrogen source	767:781	hydrogen source	767:781	The Pd/TiO2@N-C catalyst is very active and shows excellent stability towards hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source.
24861954	5	13	dep	Pd/N-C	912:917	arg1	catalyst					922:929	a catalyst	920:929	a catalyst for hydrogenation of vanillin	920:959	This activity can be attributed to a synergistic effect between the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites.
24861954	2	14	theme	large	407:411	arg1	porosity					413:420	large porosity	407:420	large porosity	407:420	N2 sorption isotherms show that the catalyst has a high BET surface area (229 m(2) g(-1)) and large porosity.
24861954	3	15	theme	porous	585:590	arg1	carbon					592:597	nitrogen-modified porous carbon	567:597	nitrogen-modified porous carbon	567:597	XPS and TEM characterization of the catalyst shows that palladium species with different chemical states are well dispersed across the TiO2 and nitrogen-modified porous carbon, respectively.
24861954	3	16	theme	palladium	479:487	arg1	species					489:495	palladium species	479:495	palladium species with different chemical states	479:526	XPS and TEM characterization of the catalyst shows that palladium species with different chemical states are well dispersed across the TiO2 and nitrogen-modified porous carbon, respectively.
24861954	3	17	theme	catalyst	459:466	arg1	TEM					431:433	TEM	431:433	TEM	431:433	XPS and TEM characterization of the catalyst shows that palladium species with different chemical states are well dispersed across the TiO2 and nitrogen-modified porous carbon, respectively.
24861954	3	17	theme	catalyst	459:466	arg1	XPS					423:425	XPS	423:425	XPS	423:425	XPS and TEM characterization of the catalyst shows that palladium species with different chemical states are well dispersed across the TiO2 and nitrogen-modified porous carbon, respectively.
24861954	4	18	theme	formic	752:757	arg1	acid					759:762	formic acid	752:762	formic acid	752:762	The Pd/TiO2@N-C catalyst is very active and shows excellent stability towards hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source.
24861954	0	19	theme	biofuels	17:24	arg1	Hydrogenation					0:12	Hydrogenation	0:12	Hydrogenation of biofuels with formic acid over a palladium-based ternary catalyst with two types of active sites.	0:113	Hydrogenation of biofuels with formic acid over a palladium-based ternary catalyst with two types of active sites.
24861954	1	20	theme	palladium	261:269	arg1	salts					271:275	palladium salts	261:275	palladium salts	261:275	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	0	21	theme	sites	108:112	arg1	types					92:96	two types	88:96	two types of active sites	88:112	Hydrogenation of biofuels with formic acid over a palladium-based ternary catalyst with two types of active sites.
24861954	2	22	theme	surface	373:379	arg1	area					381:384	a high BET surface area	362:384	a high BET surface area (229 m(2) g(-1))	362:401	N2 sorption isotherms show that the catalyst has a high BET surface area (229 m(2) g(-1)) and large porosity.
24861954	3	23	theme	different	502:510	arg1	states					521:526	different chemical states	502:526	different chemical states	502:526	XPS and TEM characterization of the catalyst shows that palladium species with different chemical states are well dispersed across the TiO2 and nitrogen-modified porous carbon, respectively.
24861954	2	24	theme	BET	369:371	arg1	area					381:384	a high BET surface area	362:384	a high BET surface area (229 m(2) g(-1))	362:401	N2 sorption isotherms show that the catalyst has a high BET surface area (229 m(2) g(-1)) and large porosity.
24861954	0	25	theme	formic	31:36	arg1	acid					38:41	formic acid	31:41	formic acid over a palladium-based ternary catalyst with two types of active sites	31:112	Hydrogenation of biofuels with formic acid over a palladium-based ternary catalyst with two types of active sites.
24861954	2	26	contain	has	358:360	arg2	area					381:384	a high BET surface area	362:384	a high BET surface area (229 m(2) g(-1))	362:401	N2 sorption isotherms show that the catalyst has a high BET surface area (229 m(2) g(-1)) and large porosity.
24861954	2	26	contain	has	358:360	arg1	catalyst					349:356	the catalyst	345:356	the catalyst	345:356	N2 sorption isotherms show that the catalyst has a high BET surface area (229 m(2) g(-1)) and large porosity.
24861954	2	26	contain	has	358:360	arg2	porosity					413:420	large porosity	407:420	large porosity	407:420	N2 sorption isotherms show that the catalyst has a high BET surface area (229 m(2) g(-1)) and large porosity.
24861954	3	27	theme	chemical	512:519	arg1	states					521:526	different chemical states	502:526	different chemical states	502:526	XPS and TEM characterization of the catalyst shows that palladium species with different chemical states are well dispersed across the TiO2 and nitrogen-modified porous carbon, respectively.
24861954	1	28	theme	nitrogen-modified	195:211	arg1	N-C					236:238	Pd/TiO2@N-C	228:238	Pd/TiO2@N-C	228:238	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	1	28	theme	nitrogen-modified	195:211	arg1	carbon					220:225	nitrogen-modified porous carbon	195:225	nitrogen-modified porous carbon (Pd/TiO2@N-C)	195:239	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	1	29	theme	tetrabutyl	278:287	arg1	titanate					289:296	tetrabutyl titanate	278:296	tetrabutyl titanate	278:296	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	2	30	dep	area	381:384	arg1	g					396:396	229 m(2) g	387:396	229 m(2) g(-1)	387:400	N2 sorption isotherms show that the catalyst has a high BET surface area (229 m(2) g(-1)) and large porosity.
24861954	2	30	dep	area	381:384	arg1	-1					398:399	-1	398:399	-1	398:399	N2 sorption isotherms show that the catalyst has a high BET surface area (229 m(2) g(-1)) and large porosity.
24861954	2	31	theme	N2	313:314	arg1	isotherms					325:333	N2 sorption isotherms	313:333	N2 sorption isotherms	313:333	N2 sorption isotherms show that the catalyst has a high BET surface area (229 m(2) g(-1)) and large porosity.
24861954	5	32	theme	vanillin	952:959	arg1	hydrogenation					935:947	hydrogenation	935:947	hydrogenation of vanillin	935:959	This activity can be attributed to a synergistic effect between the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites.
24861954	1	33	from	nanoparticles	156:168	arg1	N-C					236:238	Pd/TiO2@N-C	228:238	Pd/TiO2@N-C	228:238	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	1	33	from	nanoparticles	156:168	arg1	TiO2					182:185	TiO2	182:185	TiO2	182:185	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	1	33	from	nanoparticles	156:168	arg1	carbon					220:225	nitrogen-modified porous carbon	195:225	nitrogen-modified porous carbon (Pd/TiO2@N-C)	195:239	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	1	33	from	nanoparticles	156:168	arg1	titania					173:179	titania	173:179	titania (TiO2)	173:186	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	4	34	theme	@	625:625	arg1	catalyst					630:637	The Pd/TiO2@N-C catalyst	614:637	The Pd/TiO2@N-C catalyst	614:637	The Pd/TiO2@N-C catalyst is very active and shows excellent stability towards hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source.
24861954	4	34	theme	@	625:625	arg1	active					647:652	active	647:652	active	647:652	The Pd/TiO2@N-C catalyst is very active and shows excellent stability towards hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source.
24861954	4	35	theme	vanillin	709:716	arg1	hydrogenation					692:704	hydrogenation	692:704	hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source	692:781	The Pd/TiO2@N-C catalyst is very active and shows excellent stability towards hydrogenation of vanillin to 2-methoxy-4-methylphenol using formic acid as hydrogen source.
24861954	0	36	with	Hydrogenation	0:12	arg1	acid					38:41	formic acid	31:41	formic acid over a palladium-based ternary catalyst with two types of active sites	31:112	Hydrogenation of biofuels with formic acid over a palladium-based ternary catalyst with two types of active sites.
24861954	5	37	theme	Pd/TiO2	852:858	arg1	sites					962:966	the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites	848:966	the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites	848:966	This activity can be attributed to a synergistic effect between the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites.
24861954	3	38	dep	XPS	423:425	arg1	characterization					435:450	characterization	435:450	characterization	435:450	XPS and TEM characterization of the catalyst shows that palladium species with different chemical states are well dispersed across the TiO2 and nitrogen-modified porous carbon, respectively.
24861954	1	39	theme	porous	213:218	arg1	N-C					236:238	Pd/TiO2@N-C	228:238	Pd/TiO2@N-C	228:238	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	1	39	theme	porous	213:218	arg1	carbon					220:225	nitrogen-modified porous carbon	195:225	nitrogen-modified porous carbon (Pd/TiO2@N-C)	195:239	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	3	40	theme	nitrogen-modified	567:583	arg1	carbon					592:597	nitrogen-modified porous carbon	567:597	nitrogen-modified porous carbon	567:597	XPS and TEM characterization of the catalyst shows that palladium species with different chemical states are well dispersed across the TiO2 and nitrogen-modified porous carbon, respectively.
24861954	5	41	theme	Pd/N-C	912:917	arg1	sites					962:966	the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites	848:966	the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites	848:966	This activity can be attributed to a synergistic effect between the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites.
24861954	1	42	theme	composite	117:125	arg1	catalyst					127:134	A composite catalyst	115:134	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C)	115:239	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	0	43	theme	ternary	66:72	arg1	catalyst					74:81	a palladium-based ternary catalyst	48:81	a palladium-based ternary catalyst with two types of active sites	48:112	Hydrogenation of biofuels with formic acid over a palladium-based ternary catalyst with two types of active sites.
24861954	2	44	theme	high	364:367	arg1	area					381:384	a high BET surface area	362:384	a high BET surface area (229 m(2) g(-1))	362:401	N2 sorption isotherms show that the catalyst has a high BET surface area (229 m(2) g(-1)) and large porosity.
24861954	3	45	dep	TiO2	558:561	arg1	the					554:556	the	554:556	the	554:556	XPS and TEM characterization of the catalyst shows that palladium species with different chemical states are well dispersed across the TiO2 and nitrogen-modified porous carbon, respectively.
24861954	0	46	theme	palladium-based	50:64	arg1	catalyst					74:81	a palladium-based ternary catalyst	48:81	a palladium-based ternary catalyst with two types of active sites	48:112	Hydrogenation of biofuels with formic acid over a palladium-based ternary catalyst with two types of active sites.
24861954	5	47	theme	formic	895:900	arg1	acid					902:905	formic acid	895:905	formic acid	895:905	This activity can be attributed to a synergistic effect between the Pd/TiO2 (a catalyst for dehydrogenation of formic acid) and Pd/N-C (a catalyst for hydrogenation of vanillin) sites.
24861954	1	48	theme	Pd/TiO2	228:234	arg1	N-C					236:238	Pd/TiO2@N-C	228:238	Pd/TiO2@N-C	228:238	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
24861954	1	48	theme	Pd/TiO2	228:234	arg1	carbon					220:225	nitrogen-modified porous carbon	195:225	nitrogen-modified porous carbon (Pd/TiO2@N-C)	195:239	A composite catalyst including palladium nanoparticles on titania (TiO2) and on nitrogen-modified porous carbon (Pd/TiO2@N-C) is synthesized from palladium salts, tetrabutyl titanate, and chitosan.
26256168	3	0	theme	carboxymethylation	460:477	arg1	process					479:485	carboxymethylation process	460:485	carboxymethylation process	460:485	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	5	1	theme	temperature	891:901	arg1	cellulose					852:860	cellulose	852:860	cellulose	852:860	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	5	1	theme	temperature	891:901	arg1	time					872:875	reaction time	863:875	reaction time of 7.5min and temperature of 70°C	863:909	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	1	2	theme	alkaline	147:154	arg1	treatments					170:179	alkaline and bleaching treatments	147:179	alkaline and bleaching treatments	147:179	Cellulose was extracted from brewer's spent grain (BSG) by alkaline and bleaching treatments.
26256168	3	3	theme	substitution	596:607	arg1	degree					586:591	the average degree	574:591	the average degree of substitution (DS) of the cellulose derivative	574:640	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	2	4	from	acid	301:304	arg1	medium					318:323	alkaline medium	309:323	alkaline medium with the use of a microwave reactor	309:359	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	5	5	theme	70°C	906:909	arg1	temperature					891:901	temperature	891:901	temperature	891:901	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	5	5	theme	70°C	906:909	arg1	7.5min					880:885	7.5min	880:885	7.5min	880:885	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	4	6	theme	surface	686:692	arg1	methodology					694:704	response surface methodology	677:704	response surface methodology	677:704	An optimization strategy based on response surface methodology has been used for this process.
26256168	5	7	theme	cellulose	852:860	arg1	gram					844:847	gram	844:847	gram of cellulose, reaction time of 7.5min and temperature of 70°C	844:909	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	6	8	theme	method	994:999	arg1	feasibility					939:949	the feasibility	935:949	the feasibility of a fast and efficient microwave-assisted method to synthesize carboxymethyl cellulose from cellulose isolated of brewer's spent grain	935:1085	This work demonstrated the feasibility of a fast and efficient microwave-assisted method to synthesize carboxymethyl cellulose from cellulose isolated of brewer's spent grain.
26256168	3	9	theme	reaction	495:502	arg1	time					504:507	reaction time	495:507	reaction time	495:507	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	6	10	theme	carboxymethyl	1015:1027	arg1	cellulose					1029:1037	carboxymethyl cellulose	1015:1037	carboxymethyl cellulose	1015:1037	This work demonstrated the feasibility of a fast and efficient microwave-assisted method to synthesize carboxymethyl cellulose from cellulose isolated of brewer's spent grain.
26256168	5	11	dep	yield	766:770	arg1	5g					812:813	5g	812:813	5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C	812:909	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	3	12	theme	full-factorial	364:377	arg1	design					402:407	A full-factorial 2(3) central composite design	362:407	A full-factorial 2(3) central composite design	362:407	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	1	13	theme	bleaching	160:168	arg1	treatments					170:179	alkaline and bleaching treatments	147:179	alkaline and bleaching treatments	147:179	Cellulose was extracted from brewer's spent grain (BSG) by alkaline and bleaching treatments.
26256168	3	14	theme	acid	537:540	arg1	amount					510:515	amount	510:515	amount of monochloroacetic acid	510:540	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	3	14	theme	acid	537:540	arg1	acid					537:540	monochloroacetic acid	520:540	monochloroacetic acid	520:540	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	3	14	theme	acid	537:540	arg1	time					504:507	reaction time	495:507	reaction time	495:507	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	3	14	theme	acid	537:540	arg1	temperature					555:565	reaction temperature	546:565	reaction temperature	546:565	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	3	15	theme	central	384:390	arg1	design					402:407	A full-factorial 2(3) central composite design	362:407	A full-factorial 2(3) central composite design	362:407	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	0	16	theme	Microwave-assisted	0:17	arg1	carboxymethylation					19:36	Microwave-assisted carboxymethylation	0:36	Microwave-assisted carboxymethylation of cellulose	0:49	Microwave-assisted carboxymethylation of cellulose extracted from brewer's spent grain.
26256168	3	17	theme	monochloroacetic	520:535	arg1	acid					537:540	monochloroacetic acid	520:540	monochloroacetic acid	520:540	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	5	18	theme	7.5min	880:885	arg1	cellulose					852:860	cellulose	852:860	cellulose	852:860	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	5	18	theme	7.5min	880:885	arg1	time					872:875	reaction time	863:875	reaction time of 7.5min and temperature of 70°C	863:909	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	2	19	used	used	210:213	arg2	cellulose					196:204	The extracted cellulose	182:204	The extracted cellulose	182:204	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	2	20	theme	reactor	353:359	arg1	use					334:336	the use	330:336	the use of a microwave reactor	330:359	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	0	21	theme	cellulose	41:49	arg1	carboxymethylation					19:36	Microwave-assisted carboxymethylation	0:36	Microwave-assisted carboxymethylation of cellulose	0:49	Microwave-assisted carboxymethylation of cellulose extracted from brewer's spent grain.
26256168	3	22	theme	average	578:584	arg1	degree					586:591	the average degree	574:591	the average degree of substitution (DS) of the cellulose derivative	574:640	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	2	23	theme	monochloroacetic	284:299	arg1	acid					301:304	monochloroacetic acid	284:304	monochloroacetic acid in alkaline medium with the use of a microwave reactor	284:359	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	5	24	theme	highest	785:791	arg1	DS					793:794	the highest DS	781:794	the highest DS of 1.46 follow	781:809	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	2	25	with	medium	318:323	arg1	use					334:336	the use	330:336	the use of a microwave reactor	330:359	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	3	26	theme	process	479:485	arg1	parameters					446:455	parameters	446:455	parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature	446:565	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	3	26	theme	process	479:485	arg1	amount					510:515	amount	510:515	amount of monochloroacetic acid	510:540	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	3	26	theme	process	479:485	arg1	acid					537:540	monochloroacetic acid	520:540	monochloroacetic acid	520:540	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	3	26	theme	process	479:485	arg1	temperature					555:565	reaction temperature	546:565	reaction temperature	546:565	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	3	26	theme	process	479:485	arg1	time					504:507	reaction time	495:507	reaction time	495:507	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	4	27	theme	optimization	646:657	arg1	strategy					659:666	An optimization strategy	643:666	An optimization strategy based on response surface methodology	643:704	An optimization strategy based on response surface methodology has been used for this process.
26256168	5	28	theme	optimized	742:750	arg1	conditions					752:761	The optimized conditions	738:761	The optimized conditions	738:761	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	6	29	theme	microwave-assisted	975:992	arg1	method					994:999	a fast and efficient microwave-assisted method	954:999	a fast and efficient microwave-assisted method to synthesize carboxymethyl cellulose from cellulose isolated of brewer's spent grain	954:1085	This work demonstrated the feasibility of a fast and efficient microwave-assisted method to synthesize carboxymethyl cellulose from cellulose isolated of brewer's spent grain.
26256168	3	30	theme	composite	392:400	arg1	design					402:407	A full-factorial 2(3) central composite design	362:407	A full-factorial 2(3) central composite design	362:407	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	5	31	theme	1.46	799:802	arg1	DS					793:794	the highest DS	781:794	the highest DS of 1.46 follow	781:809	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	6	32	theme	efficient	965:973	arg1	method					994:999	a fast and efficient microwave-assisted method	954:999	a fast and efficient microwave-assisted method to synthesize carboxymethyl cellulose from cellulose isolated of brewer's spent grain	954:1085	This work demonstrated the feasibility of a fast and efficient microwave-assisted method to synthesize carboxymethyl cellulose from cellulose isolated of brewer's spent grain.
26256168	5	33	theme	acid	835:838	arg1	5g					812:813	5g	812:813	5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C	812:909	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	3	34	theme	reaction	546:553	arg1	temperature					555:565	reaction temperature	546:565	reaction temperature	546:565	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	6	35	theme	spent	1075:1079	arg1	grain					1081:1085	brewer's spent grain	1066:1085	brewer's spent grain	1066:1085	This work demonstrated the feasibility of a fast and efficient microwave-assisted method to synthesize carboxymethyl cellulose from cellulose isolated of brewer's spent grain.
26256168	4	36	theme	response	677:684	arg1	methodology					694:704	response surface methodology	677:704	response surface methodology	677:704	An optimization strategy based on response surface methodology has been used for this process.
26256168	3	37	theme	derivative	631:640	arg1	DS					610:611	DS	610:611	DS	610:611	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	3	37	theme	derivative	631:640	arg1	substitution					596:607	substitution	596:607	substitution (DS) of the cellulose derivative	596:640	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	1	38	theme	spent	126:130	arg1	grain					132:136	brewer's spent grain	117:136	brewer's spent grain (BSG)	117:142	Cellulose was extracted from brewer's spent grain (BSG) by alkaline and bleaching treatments.
26256168	1	38	theme	spent	126:130	arg1	BSG					139:141	BSG	139:141	BSG	139:141	Cellulose was extracted from brewer's spent grain (BSG) by alkaline and bleaching treatments.
26256168	5	39	dep	1.46	799:802	arg1	follow					804:809	follow	804:809	follow	804:809	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	5	40	theme	reaction	863:870	arg1	cellulose					852:860	cellulose	852:860	cellulose	852:860	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	5	40	theme	reaction	863:870	arg1	time					872:875	reaction time	863:875	reaction time of 7.5min and temperature of 70°C	863:909	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	5	41	theme	monochloroacetic	818:833	arg1	acid					835:838	monochloroacetic acid	818:838	monochloroacetic acid	818:838	The optimized conditions to yield CMC with the highest DS of 1.46 follow: 5g of monochloroacetic acid per gram of cellulose, reaction time of 7.5min and temperature of 70°C.
26256168	2	42	theme	extracted	186:194	arg1	cellulose					196:204	The extracted cellulose	182:204	The extracted cellulose	182:204	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	2	43	theme	cellulose	251:259	arg1	preparation					222:232	the preparation	218:232	the preparation of carboxymethyl cellulose (CMC)	218:265	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	2	44	theme	alkaline	309:316	arg1	medium					318:323	alkaline medium	309:323	alkaline medium with the use of a microwave reactor	309:359	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	6	45	theme	fast	956:959	arg1	method					994:999	a fast and efficient microwave-assisted method	954:999	a fast and efficient microwave-assisted method to synthesize carboxymethyl cellulose from cellulose isolated of brewer's spent grain	954:1085	This work demonstrated the feasibility of a fast and efficient microwave-assisted method to synthesize carboxymethyl cellulose from cellulose isolated of brewer's spent grain.
26256168	3	46	theme	cellulose	621:629	arg1	derivative					631:640	the cellulose derivative	617:640	the cellulose derivative	617:640	A full-factorial 2(3) central composite design was applied in order to evaluate how parameters of carboxymethylation process such as reaction time, amount of monochloroacetic acid and reaction temperature affect the average degree of substitution (DS) of the cellulose derivative.
26256168	2	47	theme	carboxymethyl	237:249	arg1	CMC					262:264	CMC	262:264	CMC	262:264	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	2	47	theme	carboxymethyl	237:249	arg1	cellulose					251:259	carboxymethyl cellulose	237:259	carboxymethyl cellulose (CMC)	237:265	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	2	48	theme	microwave	343:351	arg1	reactor					353:359	a microwave reactor	341:359	a microwave reactor	341:359	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	2	49	with	reaction	270:277	arg1	acid					301:304	monochloroacetic acid	284:304	monochloroacetic acid in alkaline medium with the use of a microwave reactor	284:359	The extracted cellulose was used in the preparation of carboxymethyl cellulose (CMC) by reaction with monochloroacetic acid in alkaline medium with the use of a microwave reactor.
26256168	0	50	theme	spent	75:79	arg1	grain					81:85	brewer's spent grain	66:85	brewer's spent grain	66:85	Microwave-assisted carboxymethylation of cellulose extracted from brewer's spent grain.
26256168	4	51	used	used	715:718	arg2	strategy					659:666	An optimization strategy	643:666	An optimization strategy based on response surface methodology	643:704	An optimization strategy based on response surface methodology has been used for this process.
28865186	2	0	theme	augmentation	494:505	arg1	application					507:517	soft tissue augmentation application	482:517	soft tissue augmentation application	482:517	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	3	1	theme	great	673:677	arg1	improvement					679:689	great improvement	673:689	great improvement	673:689	HAc-nanoHAp provided great improvement to wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc.
28865186	3	2	from	comparison	759:768	arg1	wrinkles					694:701	wrinkles	694:701	wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc	694:790	HAc-nanoHAp provided great improvement to wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc.
28865186	1	3	theme	precipitation	243:255	arg1	process					257:263	an in situ precipitation process	232:263	an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product	232:343	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	2	4	theme	tissue	487:492	arg1	application					507:517	soft tissue augmentation application	482:517	soft tissue augmentation application	482:517	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	2	5	theme	mouse	583:587	arg1	skin					589:592	mouse skin	583:592	mouse skin	583:592	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	1	6	theme	HAp	167:169	arg1	hydrogel					200:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	5	7	theme	biological	1041:1050	arg1	performance					1052:1062	the biophysical and biological performance	1021:1062	the biophysical and biological performance in skin tissue	1021:1077	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	2	8	theme	rheological	524:534	arg1	behaviors					536:544	rheological behaviors	524:544	rheological behaviors	524:544	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	3	9	from	cohesiveness	743:754	arg1	comparison					759:768	comparison	759:768	comparison with that of pure HAc	759:790	HAc-nanoHAp provided great improvement to wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc.
28865186	5	10	theme	HAc-nanoHAp	933:943	arg1	filler					945:950	HAc-nanoHAp filler	933:950	HAc-nanoHAp filler	933:950	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	2	11	dep	improvement	611:621	arg1	3					600:600	3	600:600	3	600:600	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	3	12	theme	pure	783:786	arg1	HAc					788:790	pure HAc	783:790	pure HAc	783:790	HAc-nanoHAp provided great improvement to wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc.
28865186	0	13	theme	dermal	112:117	arg1	matrix					119:124	a dermal matrix	110:124	a dermal matrix	110:124	Hyaluronic acid-hydroxyapatite nanocomposite hydrogels for enhanced biophysical and biological performance in a dermal matrix.
28865186	7	14	dep	105A	1138:1141	arg1	3315-3325					1144:1152	3315-3325	1144:1152	3315-3325	1144:1152	J Biomed Mater Res Part A: 105A: 3315-3325, 2017.
28865186	2	15	theme	wrinkle	603:609	arg1	improvement					611:621	(3) wrinkle improvement	599:621	(3) wrinkle improvement in a photo-aged mouse model	599:649	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	1	16	theme	mechanical	269:278	arg1	enhancement					295:305	mechanical and biological enhancement	269:305	mechanical and biological enhancement as a soft tissue augmentation product	269:343	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	5	17	contain	has	952:954	arg1	filler					945:950	HAc-nanoHAp filler	933:950	HAc-nanoHAp filler	933:950	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	5	17	contain	has	952:954	arg2	product					1002:1008	a soft tissue augmentation product	975:1008	a soft tissue augmentation product	975:1008	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	5	17	contain	has	952:954	arg2	potential					962:970	great potential	956:970	great potential	956:970	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	5	18	theme	skin	1067:1070	arg1	tissue					1072:1077	skin tissue	1067:1077	skin tissue	1067:1077	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	2	19	theme	HAc	465:467	arg1	hydrogel					469:476	pure HAc hydrogel	460:476	pure HAc hydrogel for soft tissue augmentation application	460:517	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	2	20	theme	soft	482:485	arg1	application					507:517	soft tissue augmentation application	482:517	soft tissue augmentation application	482:517	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	4	21	theme	dermal	859:864	arg1	matrix					866:871	the dermal matrix	855:871	the dermal matrix	855:871	HAc-nanoHAp also presented great enhancement in strengthening the dermal matrix by stimulating the synthesis of collagen and elastin.
28865186	5	22	theme	great	956:960	arg1	potential					962:970	great potential	956:970	great potential	956:970	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	5	22	theme	great	956:960	arg1	product					1002:1008	a soft tissue augmentation product	975:1008	a soft tissue augmentation product	975:1008	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	1	23	theme	nanocomposite	172:184	arg1	hydrogel					200:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	2	24	theme	pure	460:463	arg1	hydrogel					469:476	pure HAc hydrogel	460:476	pure HAc hydrogel for soft tissue augmentation application	460:517	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	2	25	theme	mouse	639:643	arg1	model					645:649	a photo-aged mouse model	626:649	a photo-aged mouse model	626:649	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	0	26	theme	Hyaluronic	0:9	arg1	acid-hydroxyapatite					11:29	Hyaluronic acid-hydroxyapatite	0:29	Hyaluronic acid-hydroxyapatite	0:29	Hyaluronic acid-hydroxyapatite nanocomposite hydrogels for enhanced biophysical and biological performance in a dermal matrix.
28865186	3	27	theme	higher	718:723	arg1	stiffness					725:733	its higher stiffness	714:733	its higher stiffness	714:733	HAc-nanoHAp provided great improvement to wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc.
28865186	3	28	from	stiffness	725:733	arg1	comparison					759:768	comparison	759:768	comparison with that of pure HAc	759:790	HAc-nanoHAp provided great improvement to wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc.
28865186	2	29	theme	photo-aged	628:637	arg1	model					645:649	a photo-aged mouse model	626:649	a photo-aged mouse model	626:649	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	1	30	theme	HAc-nanoHAp	187:197	arg1	hydrogel					200:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	3	31	from	wrinkles	694:701	arg1	comparison					759:768	comparison	759:768	comparison with that of pure HAc	759:790	HAc-nanoHAp provided great improvement to wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc.
28865186	1	32	theme	biological	284:293	arg1	enhancement					295:305	mechanical and biological enhancement	269:305	mechanical and biological enhancement as a soft tissue augmentation product	269:343	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	1	33	dep	in	235:236	arg1	situ					238:241	situ	238:241	situ	238:241	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	2	34	theme	different	419:427	arg1	perspectives					429:440	three different perspectives	413:440	three different perspectives	413:440	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	0	35	theme	enhanced	59:66	arg1	performance					95:105	enhanced biophysical and biological performance	59:105	enhanced biophysical and biological performance	59:105	Hyaluronic acid-hydroxyapatite nanocomposite hydrogels for enhanced biophysical and biological performance in a dermal matrix.
28865186	4	36	theme	elastin	918:924	arg1	synthesis					892:900	the synthesis	888:900	the synthesis of collagen and elastin	888:924	HAc-nanoHAp also presented great enhancement in strengthening the dermal matrix by stimulating the synthesis of collagen and elastin.
28865186	5	37	theme	tissue	982:987	arg1	potential					962:970	great potential	956:970	great potential	956:970	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	5	37	theme	tissue	982:987	arg1	product					1002:1008	a soft tissue augmentation product	975:1008	a soft tissue augmentation product	975:1008	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	2	38	theme	hydrogel	377:384	arg1	fillers					386:392	these composite hydrogel fillers	361:392	these composite hydrogel fillers	361:392	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	2	39	dep	diffusion	567:575	arg1	2					548:548	2	548:548	2	548:548	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	3	40	theme	cohesiveness	743:754	arg1	wrinkles					694:701	wrinkles	694:701	wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc	694:790	HAc-nanoHAp provided great improvement to wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc.
28865186	4	41	theme	collagen	905:912	arg1	synthesis					892:900	the synthesis	888:900	the synthesis of collagen and elastin	888:924	HAc-nanoHAp also presented great enhancement in strengthening the dermal matrix by stimulating the synthesis of collagen and elastin.
28865186	5	42	theme	augmentation	989:1000	arg1	potential					962:970	great potential	956:970	great potential	956:970	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	5	42	theme	augmentation	989:1000	arg1	product					1002:1008	a soft tissue augmentation product	975:1008	a soft tissue augmentation product	975:1008	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	5	43	from	performance	1052:1062	arg1	tissue					1072:1077	skin tissue	1067:1077	skin tissue	1067:1077	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	2	44	from	improvement	611:621	arg1	model					645:649	a photo-aged mouse model	626:649	a photo-aged mouse model	626:649	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	1	45	theme	soft	312:315	arg1	product					337:343	a soft tissue augmentation product	310:343	a soft tissue augmentation product	310:343	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	2	46	theme	lateral	559:565	arg1	diffusion					567:575	vivo lateral diffusion	554:575	vivo lateral diffusion under mouse skin	554:592	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	3	47	theme	stiffness	725:733	arg1	wrinkles					694:701	wrinkles	694:701	wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc	694:790	HAc-nanoHAp provided great improvement to wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc.
28865186	7	48	dep	Res	1126:1128	arg1	A					1135:1135	Part A	1130:1135	J Biomed Mater Res Part A: 105A: 3315-3325, 2017.	1111:1159	J Biomed Mater Res Part A: 105A: 3315-3325, 2017.
28865186	7	48	dep	Res	1126:1128	arg1	105A					1138:1141	105A	1138:1141	105A	1138:1141	J Biomed Mater Res Part A: 105A: 3315-3325, 2017.
28865186	4	49	theme	great	820:824	arg1	enhancement					826:836	great enhancement	820:836	great enhancement	820:836	HAc-nanoHAp also presented great enhancement in strengthening the dermal matrix by stimulating the synthesis of collagen and elastin.
28865186	1	50	theme	hyaluronic	129:138	arg1	hydrogel					200:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	0	51	theme	biophysical	68:78	arg1	performance					95:105	enhanced biophysical and biological performance	59:105	enhanced biophysical and biological performance	59:105	Hyaluronic acid-hydroxyapatite nanocomposite hydrogels for enhanced biophysical and biological performance in a dermal matrix.
28865186	1	52	theme	tissue	317:322	arg1	product					337:343	a soft tissue augmentation product	310:343	a soft tissue augmentation product	310:343	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	2	53	theme	vivo	554:557	arg1	diffusion					567:575	vivo lateral diffusion	554:575	vivo lateral diffusion under mouse skin	554:592	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	6	54	dep	©	1080:1080	arg1	Inc.					1106:1109	Inc.	1106:1109	Inc.	1106:1109	© 2017 Wiley Periodicals, Inc.
28865186	1	55	theme	acid	140:143	arg1	hydrogel					200:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	1	56	theme	augmentation	324:335	arg1	product					337:343	a soft tissue augmentation product	310:343	a soft tissue augmentation product	310:343	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	3	57	theme	gel	739:741	arg1	cohesiveness					743:754	gel cohesiveness	739:754	gel cohesiveness	739:754	HAc-nanoHAp provided great improvement to wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc.
28865186	5	58	theme	biophysical	1025:1035	arg1	performance					1052:1062	the biophysical and biological performance	1021:1062	the biophysical and biological performance in skin tissue	1021:1077	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	3	59	with	comparison	759:768	arg1	that					775:778	that	775:778	that	775:778	HAc-nanoHAp provided great improvement to wrinkles because of its higher stiffness and gel cohesiveness in comparison with that of pure HAc.
28865186	1	60	theme	HAc	146:148	arg1	hydrogel					200:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	7	61	theme	Part	1130:1133	arg1	A					1135:1135	Part A	1130:1135	J Biomed Mater Res Part A: 105A: 3315-3325, 2017.	1111:1159	J Biomed Mater Res Part A: 105A: 3315-3325, 2017.
28865186	2	62	from	behaviors	536:544	arg1	model					645:649	a photo-aged mouse model	626:649	a photo-aged mouse model	626:649	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	2	63	dep	analyzed	399:406	arg1	improvement					611:621	(3) wrinkle improvement	599:621	(3) wrinkle improvement in a photo-aged mouse model	599:649	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	2	63	dep	analyzed	399:406	arg1	behaviors					536:544	rheological behaviors	524:544	rheological behaviors	524:544	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	2	64	theme	composite	367:375	arg1	fillers					386:392	these composite hydrogel fillers	361:392	these composite hydrogel fillers	361:392	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	1	65	theme	in	235:236	arg1	process					257:263	an in situ precipitation process	232:263	an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product	232:343	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	0	66	theme	biological	84:93	arg1	performance					95:105	enhanced biophysical and biological performance	59:105	enhanced biophysical and biological performance	59:105	Hyaluronic acid-hydroxyapatite nanocomposite hydrogels for enhanced biophysical and biological performance in a dermal matrix.
28865186	5	67	theme	soft	977:980	arg1	potential					962:970	great potential	956:970	great potential	956:970	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	5	67	theme	soft	977:980	arg1	product					1002:1008	a soft tissue augmentation product	975:1008	a soft tissue augmentation product	975:1008	Thus, HAc-nanoHAp filler has great potential as a soft tissue augmentation product, improving the biophysical and biological performance in skin tissue.
28865186	1	68	theme	-hydroxyapatite	150:164	arg1	hydrogel					200:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel	127:207	A hyaluronic acid (HAc)-hydroxyapatite (HAp) nanocomposite (HAc-nanoHAp) hydrogel was fabricated through an in situ precipitation process for mechanical and biological enhancement as a soft tissue augmentation product.
28865186	2	69	dep	behaviors	536:544	arg1	1					521:521	1	521:521	1	521:521	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
28865186	2	69	dep	behaviors	536:544	arg1	diffusion					567:575	vivo lateral diffusion	554:575	vivo lateral diffusion under mouse skin	554:592	In this study, these composite hydrogel fillers were analyzed from three different perspectives and compared with pure HAc hydrogel for soft tissue augmentation application: (1) rheological behaviors, (2) in vivo lateral diffusion under mouse skin, and (3) wrinkle improvement in a photo-aged mouse model.
25761464	0	0	theme	in	104:105	arg1	mineralization					112:125	in situ mineralization	104:125	in situ mineralization for bone tissue engineering	104:153	Alginate/nanohydroxyapatite scaffolds with designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering.
25761464	8	1	theme	tissue	1716:1721	arg1	application					1735:1745	bone tissue engineering application	1711:1745	bone tissue engineering application	1711:1745	The results demonstrated that the method provides an interesting option for bone tissue engineering application.
25761464	4	2	theme	fabricated	988:997	arg1	scaffolds					999:1007	the fabricated scaffolds	984:1007	the fabricated scaffolds	984:1007	Light microscopy, scanning electron microscopy, microcomputer tomography, X-ray diffraction, and Fourier transform infrared spectroscopy were applied to characterize the fabricated scaffolds.
25761464	2	3	theme	polymer	401:407	arg1	scaffolds					409:417	incubating polymer scaffolds	390:417	incubating polymer scaffolds in simulated body fluid	390:441	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	2	4	with	mixing	352:357	arg1	polymers					378:385	polymers	378:385	polymers	378:385	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	2	4	with	mixing	352:357	arg1	scaffolds					409:417	incubating polymer scaffolds	390:417	incubating polymer scaffolds in simulated body fluid	390:441	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	2	5	theme	incubation	538:547	arg1	times					529:533	long times	524:533	long times of incubation	524:547	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	7	6	theme	pure	1581:1584	arg1	scaffolds					1595:1603	pure alginate scaffolds	1581:1603	pure alginate scaffolds without nano-HAP shell layer	1581:1632	The surface mineralization enhanced the mechanical properties and improved the cell attachment and spreading, as well as supported sustaining protein release, compared to pure alginate scaffolds without nano-HAP shell layer.
25761464	6	7	theme	strands	1372:1378	arg1	surface					1348:1354	the surface	1344:1354	the surface of the alginate strands	1344:1378	The obtained data indicate that this method is suitable to fabricate alginate/nano-HAP composite scaffolds with a layer of nano-HAP, coating the surface of the alginate strands homogeneously and completely.
25761464	1	8	theme	polymer/hydroxyapatite	188:209	arg1	scaffolds					166:174	Composite scaffolds	156:174	Composite scaffolds	156:174	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	1	8	theme	polymer/hydroxyapatite	188:209	arg1	HAP					212:214	HAP	212:214	HAP	212:214	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	1	8	theme	polymer/hydroxyapatite	188:209	arg1	scaffolds					227:235	especially polymer/hydroxyapatite (HAP) composite scaffolds	177:235	especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures	177:263	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	5	9	link	bone-marrow-derived	1151:1169	arg1	hBMSC					1195:1199	hBMSC	1195:1199	hBMSC	1195:1199	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	5	9	link	bone-marrow-derived	1151:1169	arg1	cells					1188:1192	human bone-marrow-derived mesenchymal stem cells	1145:1192	human bone-marrow-derived mesenchymal stem cells (hBMSC)	1145:1200	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	1	10	theme	bone	294:297	arg1	engineering					306:316	bone tissue engineering	294:316	bone tissue engineering	294:316	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	7	11	theme	alginate	1586:1593	arg1	scaffolds					1595:1603	pure alginate scaffolds	1581:1603	pure alginate scaffolds without nano-HAP shell layer	1581:1632	The surface mineralization enhanced the mechanical properties and improved the cell attachment and spreading, as well as supported sustaining protein release, compared to pure alginate scaffolds without nano-HAP shell layer.
25761464	2	12	theme	incubating	390:399	arg1	scaffolds					409:417	incubating polymer scaffolds	390:417	incubating polymer scaffolds in simulated body fluid	390:441	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	3	13	theme	designed	607:614	arg1	parameters					621:630	designed pore parameters	607:630	designed pore parameters	607:630	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	4	14	dep	Fourier	915:921	arg1	transform					923:931	transform	923:931	transform infrared spectroscopy	923:953	Light microscopy, scanning electron microscopy, microcomputer tomography, X-ray diffraction, and Fourier transform infrared spectroscopy were applied to characterize the fabricated scaffolds.
25761464	1	15	theme	tissue	299:304	arg1	engineering					306:316	bone tissue engineering	294:316	bone tissue engineering	294:316	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	2	16	theme	HAP	362:364	arg1	powder					366:371	HAP powder	362:371	HAP powder	362:371	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	7	17	theme	protein	1552:1558	arg1	release					1560:1566	protein release	1552:1566	protein release	1552:1566	The surface mineralization enhanced the mechanical properties and improved the cell attachment and spreading, as well as supported sustaining protein release, compared to pure alginate scaffolds without nano-HAP shell layer.
25761464	3	18	theme	pore	616:619	arg1	parameters					621:630	designed pore parameters	607:630	designed pore parameters	607:630	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	1	19	theme	Composite	156:164	arg1	materials					280:288	promising materials	270:288	promising materials for bone tissue engineering	270:316	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	1	19	theme	Composite	156:164	arg1	scaffolds					227:235	especially polymer/hydroxyapatite (HAP) composite scaffolds	177:235	especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures	177:263	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	1	19	theme	Composite	156:164	arg1	scaffolds					166:174	Composite scaffolds	156:174	Composite scaffolds	156:174	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	0	20	theme	bone	131:134	arg1	engineering					143:153	bone tissue engineering	131:153	bone tissue engineering	131:153	Alginate/nanohydroxyapatite scaffolds with designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering.
25761464	3	21	theme	organic	799:805	arg1	solvents					807:814	any organic solvents	795:814	any organic solvents	795:814	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	2	22	from	polymers	378:385	arg1	fluid					437:441	simulated body fluid	422:441	simulated body fluid	422:441	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	2	23	theme	Various	319:325	arg1	methods					327:333	Various methods	319:333	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds	319:487	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	2	23	theme	Various	319:325	arg1	mixing					352:357	direct mixing	345:357	direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid	345:441	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	3	24	dep	using	674:678	arg1	temperature					760:770	room temperature	755:770	room temperature	755:770	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	3	24	dep	using	674:678	arg1	use					788:790	the use	784:790	the use of any organic solvents	784:814	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	4	25	theme	scanning	836:843	arg1	microscopy					854:863	scanning electron microscopy	836:863	scanning electron microscopy	836:863	Light microscopy, scanning electron microscopy, microcomputer tomography, X-ray diffraction, and Fourier transform infrared spectroscopy were applied to characterize the fabricated scaffolds.
25761464	3	26	theme	mild	735:738	arg1	conditions					740:749	mild conditions	735:749	mild conditions	735:749	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	0	27	dep	in	104:105	arg1	situ					107:110	situ	107:110	situ	107:110	Alginate/nanohydroxyapatite scaffolds with designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering.
25761464	2	28	theme	body	432:435	arg1	fluid					437:441	simulated body fluid	422:441	simulated body fluid	422:441	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	4	29	dep	transform	923:931	arg1	infrared					933:940	infrared	933:940	transform infrared spectroscopy	923:953	Light microscopy, scanning electron microscopy, microcomputer tomography, X-ray diffraction, and Fourier transform infrared spectroscopy were applied to characterize the fabricated scaffolds.
25761464	7	30	theme	shell	1622:1626	arg1	layer					1628:1632	nano-HAP shell layer	1613:1632	nano-HAP shell layer	1613:1632	The surface mineralization enhanced the mechanical properties and improved the cell attachment and spreading, as well as supported sustaining protein release, compared to pure alginate scaffolds without nano-HAP shell layer.
25761464	1	31	theme	composite	217:225	arg1	scaffolds					166:174	Composite scaffolds	156:174	Composite scaffolds	156:174	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	1	31	theme	composite	217:225	arg1	HAP					212:214	HAP	212:214	HAP	212:214	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	1	31	theme	composite	217:225	arg1	scaffolds					227:235	especially polymer/hydroxyapatite (HAP) composite scaffolds	177:235	especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures	177:263	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	0	32	theme	designed	43:50	arg1	structures					63:72	designed core/shell structures	43:72	designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering	43:153	Alginate/nanohydroxyapatite scaffolds with designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering.
25761464	7	33	theme	mechanical	1450:1459	arg1	properties					1461:1470	the mechanical properties	1446:1470	the mechanical properties	1446:1470	The surface mineralization enhanced the mechanical properties and improved the cell attachment and spreading, as well as supported sustaining protein release, compared to pure alginate scaffolds without nano-HAP shell layer.
25761464	2	34	from	scaffolds	409:417	arg1	fluid					437:441	simulated body fluid	422:441	simulated body fluid	422:441	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	4	35	theme	Light	818:822	arg1	microscopy					824:833	Light microscopy	818:833	Light microscopy	818:833	Light microscopy, scanning electron microscopy, microcomputer tomography, X-ray diffraction, and Fourier transform infrared spectroscopy were applied to characterize the fabricated scaffolds.
25761464	5	36	theme	cell	1101:1104	arg1	response					1106:1113	the cell response	1097:1113	the cell response to the scaffolds	1097:1130	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	5	37	theme	scaffolds	1060:1068	arg1	properties					1021:1030	Mechanical properties	1010:1030	Mechanical properties	1010:1030	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	5	37	theme	scaffolds	1060:1068	arg1	delivery					1044:1051	protein delivery	1036:1051	protein delivery	1036:1051	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	3	38	theme	solvents	807:814	arg1	temperature					760:770	room temperature	755:770	room temperature	755:770	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	3	38	theme	solvents	807:814	arg1	use					788:790	the use	784:790	the use of any organic solvents	784:814	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	0	39	theme	tissue	136:141	arg1	engineering					143:153	bone tissue engineering	131:153	bone tissue engineering	131:153	Alginate/nanohydroxyapatite scaffolds with designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering.
25761464	5	40	theme	bone-marrow-derived	1151:1169	arg1	hBMSC					1195:1199	hBMSC	1195:1199	hBMSC	1195:1199	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	5	40	theme	bone-marrow-derived	1151:1169	arg1	cells					1188:1192	human bone-marrow-derived mesenchymal stem cells	1145:1192	human bone-marrow-derived mesenchymal stem cells (hBMSC)	1145:1200	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	2	41	theme	direct	345:350	arg1	mixing					352:357	direct mixing	345:357	direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid	345:441	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	2	42	theme	composite	469:477	arg1	scaffolds					479:487	polymer/HAP composite scaffolds	457:487	polymer/HAP composite scaffolds	457:487	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	3	43	with	scaffolds	592:600	arg1	parameters					621:630	designed pore parameters	607:630	designed pore parameters	607:630	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	3	43	with	scaffolds	592:600	arg1	structures					647:656	core/shell structures	636:656	core/shell structures	636:656	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	7	44	dep	supported	1531:1539	arg1	compared					1569:1576	compared	1569:1576	compared to pure alginate scaffolds without nano-HAP shell layer	1569:1632	The surface mineralization enhanced the mechanical properties and improved the cell attachment and spreading, as well as supported sustaining protein release, compared to pure alginate scaffolds without nano-HAP shell layer.
25761464	1	45	with	scaffolds	227:235	arg1	structures					254:263	predesigned structures	242:263	predesigned structures	242:263	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	1	46	theme	predesigned	242:252	arg1	structures					254:263	predesigned structures	242:263	predesigned structures	242:263	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	4	47	theme	X-ray	892:896	arg1	diffraction					898:908	X-ray diffraction	892:908	X-ray diffraction	892:908	Light microscopy, scanning electron microscopy, microcomputer tomography, X-ray diffraction, and Fourier transform infrared spectroscopy were applied to characterize the fabricated scaffolds.
25761464	2	48	theme	simulated	422:430	arg1	fluid					437:441	simulated body fluid	422:441	simulated body fluid	422:441	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	2	49	theme	polymer/HAP	457:467	arg1	scaffolds					479:487	polymer/HAP composite scaffolds	457:487	polymer/HAP composite scaffolds	457:487	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	0	50	theme	core/shell	52:61	arg1	structures					63:72	designed core/shell structures	43:72	designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering	43:153	Alginate/nanohydroxyapatite scaffolds with designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering.
25761464	5	51	theme	stem	1183:1186	arg1	hBMSC					1195:1199	hBMSC	1195:1199	hBMSC	1195:1199	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	5	51	theme	stem	1183:1186	arg1	cells					1188:1192	human bone-marrow-derived mesenchymal stem cells	1145:1192	human bone-marrow-derived mesenchymal stem cells (hBMSC)	1145:1200	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	3	52	theme	3D	680:681	arg1	technique					692:700	3D plotting technique	680:700	3D plotting technique	680:700	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	3	53	dep	in	706:707	arg1	situ					709:712	situ	709:712	situ	709:712	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	2	54	theme	long	524:527	arg1	times					529:533	long times	524:533	long times of incubation	524:547	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	8	55	theme	interesting	1688:1698	arg1	option					1700:1705	an interesting option	1685:1705	an interesting option for bone tissue engineering application	1685:1745	The results demonstrated that the method provides an interesting option for bone tissue engineering application.
25761464	8	56	theme	bone	1711:1714	arg1	application					1735:1745	bone tissue engineering application	1711:1745	bone tissue engineering application	1711:1745	The results demonstrated that the method provides an interesting option for bone tissue engineering application.
25761464	7	57	theme	cell	1489:1492	arg1	attachment					1494:1503	the cell attachment	1485:1503	the cell attachment	1485:1503	The surface mineralization enhanced the mechanical properties and improved the cell attachment and spreading, as well as supported sustaining protein release, compared to pure alginate scaffolds without nano-HAP shell layer.
25761464	5	58	theme	Mechanical	1010:1019	arg1	properties					1021:1030	Mechanical properties	1010:1030	Mechanical properties	1010:1030	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	3	59	theme	core/shell	636:645	arg1	structures					647:656	core/shell structures	636:656	core/shell structures	636:656	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	5	60	theme	protein	1036:1042	arg1	delivery					1044:1051	protein delivery	1036:1051	protein delivery	1036:1051	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	3	61	theme	room	755:758	arg1	temperature					760:770	room temperature	755:770	room temperature	755:770	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	6	62	theme	nano-HAP	1326:1333	arg1	layer					1317:1321	a layer	1315:1321	a layer of nano-HAP	1315:1333	The obtained data indicate that this method is suitable to fabricate alginate/nano-HAP composite scaffolds with a layer of nano-HAP, coating the surface of the alginate strands homogeneously and completely.
25761464	6	63	theme	obtained	1207:1214	arg1	data					1216:1219	The obtained data	1203:1219	The obtained data	1203:1219	The obtained data indicate that this method is suitable to fabricate alginate/nano-HAP composite scaffolds with a layer of nano-HAP, coating the surface of the alginate strands homogeneously and completely.
25761464	8	64	theme	engineering	1723:1733	arg1	application					1735:1745	bone tissue engineering application	1711:1745	bone tissue engineering application	1711:1745	The results demonstrated that the method provides an interesting option for bone tissue engineering application.
25761464	4	65	theme	electron	845:852	arg1	microscopy					854:863	scanning electron microscopy	836:863	scanning electron microscopy	836:863	Light microscopy, scanning electron microscopy, microcomputer tomography, X-ray diffraction, and Fourier transform infrared spectroscopy were applied to characterize the fabricated scaffolds.
25761464	7	66	theme	nano-HAP	1613:1620	arg1	layer					1628:1632	nano-HAP shell layer	1613:1632	nano-HAP shell layer	1613:1632	The surface mineralization enhanced the mechanical properties and improved the cell attachment and spreading, as well as supported sustaining protein release, compared to pure alginate scaffolds without nano-HAP shell layer.
25761464	3	67	theme	in	706:707	arg1	mineralization					714:727	in situ mineralization	706:727	in situ mineralization	706:727	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	5	68	theme	mesenchymal	1171:1181	arg1	hBMSC					1195:1199	hBMSC	1195:1199	hBMSC	1195:1199	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	5	68	theme	mesenchymal	1171:1181	arg1	cells					1188:1192	human bone-marrow-derived mesenchymal stem cells	1145:1192	human bone-marrow-derived mesenchymal stem cells (hBMSC)	1145:1200	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	2	69	theme	powder	366:371	arg1	mixing					352:357	direct mixing	345:357	direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid	345:441	Various methods including direct mixing of HAP powder with polymers or incubating polymer scaffolds in simulated body fluid for preparing polymer/HAP composite scaffolds are either uncontrolled or require long times of incubation.
25761464	7	70	theme	surface	1414:1420	arg1	mineralization					1422:1435	The surface mineralization	1410:1435	The surface mineralization	1410:1435	The surface mineralization enhanced the mechanical properties and improved the cell attachment and spreading, as well as supported sustaining protein release, compared to pure alginate scaffolds without nano-HAP shell layer.
25761464	6	71	theme	composite	1290:1298	arg1	scaffolds					1300:1308	alginate/nano-HAP composite scaffolds	1272:1308	alginate/nano-HAP composite scaffolds	1272:1308	The obtained data indicate that this method is suitable to fabricate alginate/nano-HAP composite scaffolds with a layer of nano-HAP, coating the surface of the alginate strands homogeneously and completely.
25761464	5	72	theme	human	1145:1149	arg1	hBMSC					1195:1199	hBMSC	1195:1199	hBMSC	1195:1199	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	5	72	theme	human	1145:1149	arg1	cells					1188:1192	human bone-marrow-derived mesenchymal stem cells	1145:1192	human bone-marrow-derived mesenchymal stem cells (hBMSC)	1145:1200	Mechanical properties and protein delivery of the scaffolds were evaluated, as well as the cell response to the scaffolds by culturing human bone-marrow-derived mesenchymal stem cells (hBMSC).
25761464	3	73	theme	plotting	683:690	arg1	technique					692:700	3D plotting technique	680:700	3D plotting technique	680:700	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	3	74	theme	alginate/nano-HAP	564:580	arg1	scaffolds					592:600	alginate/nano-HAP composite scaffolds	564:600	alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures	564:656	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	1	75	theme	promising	270:278	arg1	materials					280:288	promising materials	270:288	promising materials for bone tissue engineering	270:316	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	1	75	theme	promising	270:278	arg1	scaffolds					166:174	Composite scaffolds	156:174	Composite scaffolds	156:174	Composite scaffolds, especially polymer/hydroxyapatite (HAP) composite scaffolds with predesigned structures, are promising materials for bone tissue engineering.
25761464	0	76	theme	3D	88:89	arg1	plotting					91:98	3D plotting	88:98	3D plotting	88:98	Alginate/nanohydroxyapatite scaffolds with designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering.
25761464	4	77	theme	microcomputer	866:878	arg1	tomography					880:889	microcomputer tomography	866:889	microcomputer tomography	866:889	Light microscopy, scanning electron microscopy, microcomputer tomography, X-ray diffraction, and Fourier transform infrared spectroscopy were applied to characterize the fabricated scaffolds.
25761464	6	78	theme	alginate/nano-HAP	1272:1288	arg1	scaffolds					1300:1308	alginate/nano-HAP composite scaffolds	1272:1308	alginate/nano-HAP composite scaffolds	1272:1308	The obtained data indicate that this method is suitable to fabricate alginate/nano-HAP composite scaffolds with a layer of nano-HAP, coating the surface of the alginate strands homogeneously and completely.
25761464	3	79	theme	composite	582:590	arg1	scaffolds					592:600	alginate/nano-HAP composite scaffolds	564:600	alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures	564:656	In this work, alginate/nano-HAP composite scaffolds with designed pore parameters and core/shell structures were fabricated using 3D plotting technique and in situ mineralization under mild conditions (at room temperature and without the use of any organic solvents).
25761464	6	80	theme	alginate	1363:1370	arg1	strands					1372:1378	the alginate strands	1359:1378	the alginate strands	1359:1378	The obtained data indicate that this method is suitable to fabricate alginate/nano-HAP composite scaffolds with a layer of nano-HAP, coating the surface of the alginate strands homogeneously and completely.
27101258	7	0	theme	primary	1094:1100	arg1	fibroblasts					1115:1125	primary mouse embryo fibroblasts	1094:1125	primary mouse embryo fibroblasts	1094:1125	By contrast, normal cells appeared to be stimulated rather than inhibited by the peptide, whereas primary mouse embryo fibroblasts behaved similarly to tumour cells.
27101258	3	1	theme	gas	526:528	arg1	chromatography					530:543	gas chromatography	526:543	gas chromatography	526:543	The most active fractions were then lyophilized and the residue was analysed by gas chromatography for its amino acid content and composition.
27101258	5	2	theme	CH-DE-FR-GB	746:756	arg1	number					766:771	(CH-DE-FR-GB) Patent number 1217005	745:779	(CH-DE-FR-GB) Patent number 1217005; IT number 01320177]	745:800	(CH-DE-FR-GB) Patent number 1217005; IT number 01320177] and its cytotoxicity was tested against tumoural and normal cells.
27101258	1	3	theme	casei	262:266	arg1	peptidoglycan					231:243	the peptidoglycan	227:243	the peptidoglycan of Lactobacillus casei	227:266	In a previous study, we reported the cytotoxic activity against various tumour cells of the peptidoglycan of Lactobacillus casei.
27101258	7	4	theme	mouse	1102:1106	arg1	fibroblasts					1115:1125	primary mouse embryo fibroblasts	1094:1125	primary mouse embryo fibroblasts	1094:1125	By contrast, normal cells appeared to be stimulated rather than inhibited by the peptide, whereas primary mouse embryo fibroblasts behaved similarly to tumour cells.
27101258	2	5	theme	complex	375:381	arg1	separation					343:352	column-chromatography separation	321:352	column-chromatography separation of the peptidoglycan complex	321:381	To isolate the most active components, we performed column-chromatography separation of the peptidoglycan complex and tested the related fractions for their cytotoxic activity.
27101258	6	6	theme	apoptotic	977:985	arg1	process					987:993	the apoptotic process	973:993	the apoptotic process	973:993	The synthetic peptide was found to impair the entire metabolism of cultured tumour cells and to restore the apoptotic process.
27101258	4	7	theme	known	609:613	arg1	formula					624:630	the known chemical formula	605:630	the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei	605:703	On the basis of the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei, a peptide was then synthesized [Europ.
27101258	2	8	theme	active	289:294	arg1	components					296:305	the most active components	280:305	the most active components	280:305	To isolate the most active components, we performed column-chromatography separation of the peptidoglycan complex and tested the related fractions for their cytotoxic activity.
27101258	8	9	theme	constituent	1236:1246	arg1	peptide					1254:1260	their constituent basic peptide	1230:1260	their constituent basic peptide	1230:1260	On the basis of these results, L. casei peptidoglycan fragments and their constituent basic peptide might be applicable as potent antitumour agents.
27101258	5	10	dep	number	785:790	arg1	IT					782:783	IT	782:783	IT	782:783	(CH-DE-FR-GB) Patent number 1217005; IT number 01320177] and its cytotoxicity was tested against tumoural and normal cells.
27101258	4	11	theme	basic	639:643	arg1	component					654:662	the basic peptidic component	635:662	the basic peptidic component of the peptidoglycan complex of L. casei	635:703	On the basis of the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei, a peptide was then synthesized [Europ.
27101258	3	12	theme	amino	553:557	arg1	content					564:570	its amino acid content	549:570	its amino acid content	549:570	The most active fractions were then lyophilized and the residue was analysed by gas chromatography for its amino acid content and composition.
27101258	4	13	theme	peptidoglycan	671:683	arg1	complex					685:691	the peptidoglycan complex	667:691	the peptidoglycan complex of L. casei	667:703	On the basis of the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei, a peptide was then synthesized [Europ.
27101258	0	14	theme	mitochondrial-bound	107:125	arg1	hexokinase					127:136	mitochondrial-bound hexokinase	107:136	mitochondrial-bound hexokinase	107:136	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan and its interaction with mitochondrial-bound hexokinase.
27101258	6	15	theme	synthetic	873:881	arg1	peptide					883:889	The synthetic peptide	869:889	The synthetic peptide	869:889	The synthetic peptide was found to impair the entire metabolism of cultured tumour cells and to restore the apoptotic process.
27101258	8	16	theme	results	1184:1190	arg1	applicable					1271:1280	applicable	1271:1280	applicable	1271:1280	On the basis of these results, L. casei peptidoglycan fragments and their constituent basic peptide might be applicable as potent antitumour agents.
27101258	3	17	theme	acid	559:562	arg1	content					564:570	its amino acid content	549:570	its amino acid content	549:570	The most active fractions were then lyophilized and the residue was analysed by gas chromatography for its amino acid content and composition.
27101258	1	18	theme	cytotoxic	176:184	arg1	activity					186:193	the cytotoxic activity	172:193	the cytotoxic activity against various tumour cells of the peptidoglycan of Lactobacillus casei	172:266	In a previous study, we reported the cytotoxic activity against various tumour cells of the peptidoglycan of Lactobacillus casei.
27101258	2	19	theme	column-chromatography	321:341	arg1	separation					343:352	column-chromatography separation	321:352	column-chromatography separation of the peptidoglycan complex	321:381	To isolate the most active components, we performed column-chromatography separation of the peptidoglycan complex and tested the related fractions for their cytotoxic activity.
27101258	0	20	theme	Antitumoural	0:11	arg1	activity					13:20	Antitumoural activity	0:20	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan	0:80	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan and its interaction with mitochondrial-bound hexokinase.
27101258	8	21	theme	peptidoglycan	1202:1214	arg1	fragments					1216:1224	L. casei peptidoglycan fragments	1193:1224	L. casei peptidoglycan fragments	1193:1224	On the basis of these results, L. casei peptidoglycan fragments and their constituent basic peptide might be applicable as potent antitumour agents.
27101258	2	22	theme	related	398:404	arg1	fractions					406:414	the related fractions	394:414	the related fractions for their cytotoxic activity	394:443	To isolate the most active components, we performed column-chromatography separation of the peptidoglycan complex and tested the related fractions for their cytotoxic activity.
27101258	5	23	dep	number	766:771	arg1	number					785:790	number 01320177	785:799	number 01320177	785:799	(CH-DE-FR-GB) Patent number 1217005; IT number 01320177] and its cytotoxicity was tested against tumoural and normal cells.
27101258	0	24	with	interaction	90:100	arg1	hexokinase					127:136	mitochondrial-bound hexokinase	107:136	mitochondrial-bound hexokinase	107:136	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan and its interaction with mitochondrial-bound hexokinase.
27101258	1	25	theme	various	203:209	arg1	cells					218:222	various tumour cells	203:222	various tumour cells of the peptidoglycan of Lactobacillus casei	203:266	In a previous study, we reported the cytotoxic activity against various tumour cells of the peptidoglycan of Lactobacillus casei.
27101258	7	26	theme	tumour	1148:1153	arg1	cells					1155:1159	tumour cells	1148:1159	tumour cells	1148:1159	By contrast, normal cells appeared to be stimulated rather than inhibited by the peptide, whereas primary mouse embryo fibroblasts behaved similarly to tumour cells.
27101258	6	27	theme	cells	952:956	arg1	metabolism					922:931	the entire metabolism	911:931	the entire metabolism of cultured tumour cells	911:956	The synthetic peptide was found to impair the entire metabolism of cultured tumour cells and to restore the apoptotic process.
27101258	1	28	theme	tumour	211:216	arg1	cells					218:222	various tumour cells	203:222	various tumour cells of the peptidoglycan of Lactobacillus casei	203:266	In a previous study, we reported the cytotoxic activity against various tumour cells of the peptidoglycan of Lactobacillus casei.
27101258	0	29	theme	peptide	37:43	arg1	activity					13:20	Antitumoural activity	0:20	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan	0:80	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan and its interaction with mitochondrial-bound hexokinase.
27101258	0	29	theme	peptide	37:43	arg1	interaction					90:100	its interaction	86:100	its interaction with mitochondrial-bound hexokinase	86:136	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan and its interaction with mitochondrial-bound hexokinase.
27101258	6	30	theme	tumour	945:950	arg1	cells					952:956	cultured tumour cells	936:956	cultured tumour cells	936:956	The synthetic peptide was found to impair the entire metabolism of cultured tumour cells and to restore the apoptotic process.
27101258	8	31	dep	results	1184:1190	arg1	basis					1169:1173	basis	1169:1173	basis	1169:1173	On the basis of these results, L. casei peptidoglycan fragments and their constituent basic peptide might be applicable as potent antitumour agents.
27101258	8	31	dep	results	1184:1190	arg1	the					1165:1167	the	1165:1167	the	1165:1167	On the basis of these results, L. casei peptidoglycan fragments and their constituent basic peptide might be applicable as potent antitumour agents.
27101258	3	32	theme	active	455:460	arg1	fractions					462:470	The most active fractions	446:470	The most active fractions	446:470	The most active fractions were then lyophilized and the residue was analysed by gas chromatography for its amino acid content and composition.
27101258	7	33	theme	embryo	1108:1113	arg1	fibroblasts					1115:1125	primary mouse embryo fibroblasts	1094:1125	primary mouse embryo fibroblasts	1094:1125	By contrast, normal cells appeared to be stimulated rather than inhibited by the peptide, whereas primary mouse embryo fibroblasts behaved similarly to tumour cells.
27101258	0	34	theme	cytotoxic	27:35	arg1	peptide					37:43	a cytotoxic peptide	25:43	a cytotoxic peptide of Lactobacillus casei peptidoglycan	25:80	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan and its interaction with mitochondrial-bound hexokinase.
27101258	6	35	theme	cultured	936:943	arg1	cells					952:956	cultured tumour cells	936:956	cultured tumour cells	936:956	The synthetic peptide was found to impair the entire metabolism of cultured tumour cells and to restore the apoptotic process.
27101258	5	36	theme	tumoural	842:849	arg1	cells					862:866	tumoural and normal cells	842:866	tumoural and normal cells	842:866	(CH-DE-FR-GB) Patent number 1217005; IT number 01320177] and its cytotoxicity was tested against tumoural and normal cells.
27101258	2	37	theme	cytotoxic	426:434	arg1	activity					436:443	their cytotoxic activity	420:443	their cytotoxic activity	420:443	To isolate the most active components, we performed column-chromatography separation of the peptidoglycan complex and tested the related fractions for their cytotoxic activity.
27101258	0	38	theme	Lactobacillus	48:60	arg1	casei					62:66	Lactobacillus casei	48:66	Lactobacillus casei peptidoglycan	48:80	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan and its interaction with mitochondrial-bound hexokinase.
27101258	5	39	theme	Patent	759:764	arg1	number					766:771	(CH-DE-FR-GB) Patent number 1217005	745:779	(CH-DE-FR-GB) Patent number 1217005; IT number 01320177]	745:800	(CH-DE-FR-GB) Patent number 1217005; IT number 01320177] and its cytotoxicity was tested against tumoural and normal cells.
27101258	2	40	theme	peptidoglycan	361:373	arg1	complex					375:381	the peptidoglycan complex	357:381	the peptidoglycan complex	357:381	To isolate the most active components, we performed column-chromatography separation of the peptidoglycan complex and tested the related fractions for their cytotoxic activity.
27101258	4	41	theme	chemical	615:622	arg1	formula					624:630	the known chemical formula	605:630	the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei	605:703	On the basis of the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei, a peptide was then synthesized [Europ.
27101258	0	42	with	activity	13:20	arg1	hexokinase					127:136	mitochondrial-bound hexokinase	107:136	mitochondrial-bound hexokinase	107:136	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan and its interaction with mitochondrial-bound hexokinase.
27101258	4	43	theme	complex	685:691	arg1	component					654:662	the basic peptidic component	635:662	the basic peptidic component of the peptidoglycan complex of L. casei	635:703	On the basis of the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei, a peptide was then synthesized [Europ.
27101258	6	44	theme	entire	915:920	arg1	metabolism					922:931	the entire metabolism	911:931	the entire metabolism of cultured tumour cells	911:956	The synthetic peptide was found to impair the entire metabolism of cultured tumour cells and to restore the apoptotic process.
27101258	7	45	theme	normal	1009:1014	arg1	cells					1016:1020	normal cells	1009:1020	normal cells	1009:1020	By contrast, normal cells appeared to be stimulated rather than inhibited by the peptide, whereas primary mouse embryo fibroblasts behaved similarly to tumour cells.
27101258	8	46	theme	basic	1248:1252	arg1	peptide					1254:1260	their constituent basic peptide	1230:1260	their constituent basic peptide	1230:1260	On the basis of these results, L. casei peptidoglycan fragments and their constituent basic peptide might be applicable as potent antitumour agents.
27101258	4	47	dep	formula	624:630	arg1	basis					596:600	basis	596:600	basis	596:600	On the basis of the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei, a peptide was then synthesized [Europ.
27101258	4	47	dep	formula	624:630	arg1	the					592:594	the	592:594	the	592:594	On the basis of the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei, a peptide was then synthesized [Europ.
27101258	8	48	theme	antitumour	1292:1301	arg1	agents					1303:1308	potent antitumour agents	1285:1308	potent antitumour agents	1285:1308	On the basis of these results, L. casei peptidoglycan fragments and their constituent basic peptide might be applicable as potent antitumour agents.
27101258	1	49	theme	previous	144:151	arg1	study					153:157	a previous study	142:157	a previous study	142:157	In a previous study, we reported the cytotoxic activity against various tumour cells of the peptidoglycan of Lactobacillus casei.
27101258	1	50	theme	peptidoglycan	231:243	arg1	cells					218:222	various tumour cells	203:222	various tumour cells of the peptidoglycan of Lactobacillus casei	203:266	In a previous study, we reported the cytotoxic activity against various tumour cells of the peptidoglycan of Lactobacillus casei.
27101258	0	51	theme	peptidoglycan	68:80	arg1	peptide					37:43	a cytotoxic peptide	25:43	a cytotoxic peptide of Lactobacillus casei peptidoglycan	25:80	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan and its interaction with mitochondrial-bound hexokinase.
27101258	4	52	theme	component	654:662	arg1	formula					624:630	the known chemical formula	605:630	the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei	605:703	On the basis of the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei, a peptide was then synthesized [Europ.
27101258	4	53	theme	peptidic	645:652	arg1	component					654:662	the basic peptidic component	635:662	the basic peptidic component of the peptidoglycan complex of L. casei	635:703	On the basis of the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei, a peptide was then synthesized [Europ.
27101258	4	54	theme	casei	699:703	arg1	complex					685:691	the peptidoglycan complex	667:691	the peptidoglycan complex of L. casei	667:703	On the basis of the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei, a peptide was then synthesized [Europ.
27101258	5	55	theme	normal	855:860	arg1	cells					862:866	tumoural and normal cells	842:866	tumoural and normal cells	842:866	(CH-DE-FR-GB) Patent number 1217005; IT number 01320177] and its cytotoxicity was tested against tumoural and normal cells.
27101258	0	56	theme	casei	62:66	arg1	peptidoglycan					68:80	Lactobacillus casei peptidoglycan	48:80	Lactobacillus casei peptidoglycan	48:80	Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan and its interaction with mitochondrial-bound hexokinase.
27101258	8	57	theme	casei	1196:1200	arg1	fragments					1216:1224	L. casei peptidoglycan fragments	1193:1224	L. casei peptidoglycan fragments	1193:1224	On the basis of these results, L. casei peptidoglycan fragments and their constituent basic peptide might be applicable as potent antitumour agents.
27101258	8	58	theme	potent	1285:1290	arg1	agents					1303:1308	potent antitumour agents	1285:1308	potent antitumour agents	1285:1308	On the basis of these results, L. casei peptidoglycan fragments and their constituent basic peptide might be applicable as potent antitumour agents.
27101258	1	59	theme	Lactobacillus	248:260	arg1	casei					262:266	Lactobacillus casei	248:266	Lactobacillus casei	248:266	In a previous study, we reported the cytotoxic activity against various tumour cells of the peptidoglycan of Lactobacillus casei.
26104904	1	0	theme	sand-supported	145:158	arg1	composite					183:191	coarse sand-supported zero valent iron (ZVI) composite	138:191	coarse sand-supported zero valent iron (ZVI) composite	138:191	In this study, coarse sand-supported zero valent iron (ZVI) composite was synthesized by adding sodium alginate to immobilize.
26104904	4	1	theme	synthesized	470:480	arg1	composite					482:490	The synthesized composite	466:490	The synthesized composite	466:490	The synthesized composite was used for degradation of 2,4-dichlorophenol (2,4-DCP) contamination in groundwater.
26104904	5	2	theme	2,4-DCP	643:649	arg1	mechanism					630:638	degradation mechanism	618:638	degradation mechanism of 2,4-DCP using coarse sand-supported ZVI	618:681	Experimental results demonstrated that degradation mechanism of 2,4-DCP using coarse sand-supported ZVI included adsorption, desorption, and dechlorination.
26104904	4	3	theme	2,4-DCP	540:546	arg1	contamination					549:561	2,4-dichlorophenol (2,4-DCP) contamination	520:561	2,4-dichlorophenol (2,4-DCP) contamination	520:561	The synthesized composite was used for degradation of 2,4-dichlorophenol (2,4-DCP) contamination in groundwater.
26104904	0	4	theme	2,4-dichlorophenol	88:105	arg1	removal					77:83	the removal	73:83	the removal of 2,4-dichlorophenol in groundwater	73:120	Permeable reactive barrier of coarse sand-supported zero valent iron for the removal of 2,4-dichlorophenol in groundwater.
26104904	2	5	theme	scanning	276:283	arg1	microscope					294:303	scanning electron microscope	276:303	scanning electron microscope (SEM)	276:309	Composite was detected by scanning electron microscope (SEM), X-ray diffraction (XRD), and X-ray fluorescence (XRF).
26104904	2	5	theme	scanning	276:283	arg1	SEM					306:308	SEM	306:308	SEM	306:308	Composite was detected by scanning electron microscope (SEM), X-ray diffraction (XRD), and X-ray fluorescence (XRF).
26104904	5	6	theme	coarse	657:662	arg1	ZVI					679:681	coarse sand-supported ZVI	657:681	coarse sand-supported ZVI	657:681	Experimental results demonstrated that degradation mechanism of 2,4-DCP using coarse sand-supported ZVI included adsorption, desorption, and dechlorination.
26104904	5	7	theme	Experimental	579:590	arg1	results					592:598	Experimental results	579:598	Experimental results	579:598	Experimental results demonstrated that degradation mechanism of 2,4-DCP using coarse sand-supported ZVI included adsorption, desorption, and dechlorination.
26104904	4	8	theme	contamination	549:561	arg1	degradation					505:515	degradation	505:515	degradation of 2,4-dichlorophenol (2,4-DCP) contamination in groundwater	505:576	The synthesized composite was used for degradation of 2,4-dichlorophenol (2,4-DCP) contamination in groundwater.
26104904	4	9	used	used	496:499	arg2	composite					482:490	The synthesized composite	466:490	The synthesized composite	466:490	The synthesized composite was used for degradation of 2,4-dichlorophenol (2,4-DCP) contamination in groundwater.
26104904	1	10	theme	valent	165:170	arg1	composite					183:191	coarse sand-supported zero valent iron (ZVI) composite	138:191	coarse sand-supported zero valent iron (ZVI) composite	138:191	In this study, coarse sand-supported zero valent iron (ZVI) composite was synthesized by adding sodium alginate to immobilize.
26104904	7	11	from	reductants	901:910	arg1	dechlorination					915:928	dechlorination	915:928	dechlorination of 2,4-DCP using ZVI	915:949	It was concluded that dechlorination was the key reaction pathway, ZVI and hydrogen are prime reductants in dechlorination of 2,4-DCP using ZVI.
26104904	1	12	theme	iron	172:175	arg1	composite					183:191	coarse sand-supported zero valent iron (ZVI) composite	138:191	coarse sand-supported zero valent iron (ZVI) composite	138:191	In this study, coarse sand-supported zero valent iron (ZVI) composite was synthesized by adding sodium alginate to immobilize.
26104904	0	13	theme	reactive	10:17	arg1	barrier					19:25	Permeable reactive barrier	0:25	Permeable reactive barrier of coarse	0:35	Permeable reactive barrier of coarse sand-supported zero valent iron for the removal of 2,4-dichlorophenol in groundwater.
26104904	4	14	theme	2,4-dichlorophenol	520:537	arg1	contamination					549:561	2,4-dichlorophenol (2,4-DCP) contamination	520:561	2,4-dichlorophenol (2,4-DCP) contamination	520:561	The synthesized composite was used for degradation of 2,4-dichlorophenol (2,4-DCP) contamination in groundwater.
26104904	5	15	theme	degradation	618:628	arg1	mechanism					630:638	degradation mechanism	618:638	degradation mechanism of 2,4-DCP using coarse sand-supported ZVI	618:681	Experimental results demonstrated that degradation mechanism of 2,4-DCP using coarse sand-supported ZVI included adsorption, desorption, and dechlorination.
26104904	2	16	theme	X-ray	341:345	arg1	fluorescence					347:358	X-ray fluorescence	341:358	X-ray fluorescence (XRF)	341:364	Composite was detected by scanning electron microscope (SEM), X-ray diffraction (XRD), and X-ray fluorescence (XRF).
26104904	2	16	theme	X-ray	341:345	arg1	XRF					361:363	XRF	361:363	XRF	361:363	Composite was detected by scanning electron microscope (SEM), X-ray diffraction (XRD), and X-ray fluorescence (XRF).
26104904	0	17	theme	Permeable	0:8	arg1	barrier					19:25	Permeable reactive barrier	0:25	Permeable reactive barrier of coarse	0:35	Permeable reactive barrier of coarse sand-supported zero valent iron for the removal of 2,4-dichlorophenol in groundwater.
26104904	7	18	theme	key	852:854	arg1	pathway					865:871	the key reaction pathway	848:871	the key reaction pathway	848:871	It was concluded that dechlorination was the key reaction pathway, ZVI and hydrogen are prime reductants in dechlorination of 2,4-DCP using ZVI.
26104904	7	18	theme	key	852:854	arg1	dechlorination					829:842	dechlorination	829:842	dechlorination	829:842	It was concluded that dechlorination was the key reaction pathway, ZVI and hydrogen are prime reductants in dechlorination of 2,4-DCP using ZVI.
26104904	1	19	theme	ZVI	178:180	arg1	composite					183:191	coarse sand-supported zero valent iron (ZVI) composite	138:191	coarse sand-supported zero valent iron (ZVI) composite	138:191	In this study, coarse sand-supported zero valent iron (ZVI) composite was synthesized by adding sodium alginate to immobilize.
26104904	6	20	theme	kinetic	792:798	arg1	adsorption					744:753	2,4-DCP adsorption	736:753	2,4-DCP adsorption	736:753	2,4-DCP adsorption was described as pseudo-second-order kinetic model.
26104904	6	20	theme	kinetic	792:798	arg1	model					800:804	pseudo-second-order kinetic model	772:804	pseudo-second-order kinetic model	772:804	2,4-DCP adsorption was described as pseudo-second-order kinetic model.
26104904	7	21	theme	reaction	856:863	arg1	pathway					865:871	the key reaction pathway	848:871	the key reaction pathway	848:871	It was concluded that dechlorination was the key reaction pathway, ZVI and hydrogen are prime reductants in dechlorination of 2,4-DCP using ZVI.
26104904	7	21	theme	reaction	856:863	arg1	dechlorination					829:842	dechlorination	829:842	dechlorination	829:842	It was concluded that dechlorination was the key reaction pathway, ZVI and hydrogen are prime reductants in dechlorination of 2,4-DCP using ZVI.
26104904	4	22	from	degradation	505:515	arg1	groundwater					566:576	groundwater	566:576	groundwater	566:576	The synthesized composite was used for degradation of 2,4-dichlorophenol (2,4-DCP) contamination in groundwater.
26104904	0	23	from	removal	77:83	arg1	groundwater					110:120	groundwater	110:120	groundwater	110:120	Permeable reactive barrier of coarse sand-supported zero valent iron for the removal of 2,4-dichlorophenol in groundwater.
26104904	2	24	theme	electron	285:292	arg1	microscope					294:303	scanning electron microscope	276:303	scanning electron microscope (SEM)	276:309	Composite was detected by scanning electron microscope (SEM), X-ray diffraction (XRD), and X-ray fluorescence (XRF).
26104904	2	24	theme	electron	285:292	arg1	SEM					306:308	SEM	306:308	SEM	306:308	Composite was detected by scanning electron microscope (SEM), X-ray diffraction (XRD), and X-ray fluorescence (XRF).
26104904	3	25	theme	distribution	444:455	arg1	pattern					457:463	a wide porous distribution pattern	430:463	a wide porous distribution pattern	430:463	SEM results showed that composite had core-shell structure and a wide porous distribution pattern.
26104904	3	26	contain	had	401:403	arg1	composite					391:399	composite	391:399	composite	391:399	SEM results showed that composite had core-shell structure and a wide porous distribution pattern.
26104904	3	26	contain	had	401:403	arg2	pattern					457:463	a wide porous distribution pattern	430:463	a wide porous distribution pattern	430:463	SEM results showed that composite had core-shell structure and a wide porous distribution pattern.
26104904	3	26	contain	had	401:403	arg2	structure					416:424	core-shell structure	405:424	core-shell structure	405:424	SEM results showed that composite had core-shell structure and a wide porous distribution pattern.
26104904	0	27	theme	coarse	30:35	arg1	barrier					19:25	Permeable reactive barrier	0:25	Permeable reactive barrier of coarse	0:35	Permeable reactive barrier of coarse sand-supported zero valent iron for the removal of 2,4-dichlorophenol in groundwater.
26104904	3	28	theme	SEM	367:369	arg1	results					371:377	SEM results	367:377	SEM results	367:377	SEM results showed that composite had core-shell structure and a wide porous distribution pattern.
26104904	6	29	theme	pseudo-second-order	772:790	arg1	adsorption					744:753	2,4-DCP adsorption	736:753	2,4-DCP adsorption	736:753	2,4-DCP adsorption was described as pseudo-second-order kinetic model.
26104904	6	29	theme	pseudo-second-order	772:790	arg1	model					800:804	pseudo-second-order kinetic model	772:804	pseudo-second-order kinetic model	772:804	2,4-DCP adsorption was described as pseudo-second-order kinetic model.
26104904	3	30	theme	core-shell	405:414	arg1	structure					416:424	core-shell structure	405:424	core-shell structure	405:424	SEM results showed that composite had core-shell structure and a wide porous distribution pattern.
26104904	0	31	theme	valent	57:62	arg1	iron					64:67	zero valent iron	52:67	zero valent iron for the removal of 2,4-dichlorophenol in groundwater	52:120	Permeable reactive barrier of coarse sand-supported zero valent iron for the removal of 2,4-dichlorophenol in groundwater.
26104904	5	32	theme	sand-supported	664:677	arg1	ZVI					679:681	coarse sand-supported ZVI	657:681	coarse sand-supported ZVI	657:681	Experimental results demonstrated that degradation mechanism of 2,4-DCP using coarse sand-supported ZVI included adsorption, desorption, and dechlorination.
26104904	3	33	theme	porous	437:442	arg1	pattern					457:463	a wide porous distribution pattern	430:463	a wide porous distribution pattern	430:463	SEM results showed that composite had core-shell structure and a wide porous distribution pattern.
26104904	0	34	theme	zero	52:55	arg1	valent					57:62	valent	57:62	valent	57:62	Permeable reactive barrier of coarse sand-supported zero valent iron for the removal of 2,4-dichlorophenol in groundwater.
26104904	0	35	from	groundwater	110:120	arg1	removal					77:83	the removal	73:83	the removal of 2,4-dichlorophenol in groundwater	73:120	Permeable reactive barrier of coarse sand-supported zero valent iron for the removal of 2,4-dichlorophenol in groundwater.
26104904	2	36	theme	X-ray	312:316	arg1	XRD					331:333	XRD	331:333	XRD	331:333	Composite was detected by scanning electron microscope (SEM), X-ray diffraction (XRD), and X-ray fluorescence (XRF).
26104904	2	36	theme	X-ray	312:316	arg1	diffraction					318:328	X-ray diffraction	312:328	X-ray diffraction (XRD)	312:334	Composite was detected by scanning electron microscope (SEM), X-ray diffraction (XRD), and X-ray fluorescence (XRF).
26104904	7	37	theme	2,4-DCP	933:939	arg1	dechlorination					915:928	dechlorination	915:928	dechlorination of 2,4-DCP using ZVI	915:949	It was concluded that dechlorination was the key reaction pathway, ZVI and hydrogen are prime reductants in dechlorination of 2,4-DCP using ZVI.
26104904	0	38	from	2,4-dichlorophenol	88:105	arg1	groundwater					110:120	groundwater	110:120	groundwater	110:120	Permeable reactive barrier of coarse sand-supported zero valent iron for the removal of 2,4-dichlorophenol in groundwater.
26104904	3	39	theme	wide	432:435	arg1	pattern					457:463	a wide porous distribution pattern	430:463	a wide porous distribution pattern	430:463	SEM results showed that composite had core-shell structure and a wide porous distribution pattern.
26104904	6	40	theme	2,4-DCP	736:742	arg1	adsorption					744:753	2,4-DCP adsorption	736:753	2,4-DCP adsorption	736:753	2,4-DCP adsorption was described as pseudo-second-order kinetic model.
26104904	6	40	theme	2,4-DCP	736:742	arg1	model					800:804	pseudo-second-order kinetic model	772:804	pseudo-second-order kinetic model	772:804	2,4-DCP adsorption was described as pseudo-second-order kinetic model.
26104904	1	41	theme	sodium	219:224	arg1	alginate					226:233	sodium alginate	219:233	sodium alginate	219:233	In this study, coarse sand-supported zero valent iron (ZVI) composite was synthesized by adding sodium alginate to immobilize.
26104904	1	42	theme	coarse	138:143	arg1	composite					183:191	coarse sand-supported zero valent iron (ZVI) composite	138:191	coarse sand-supported zero valent iron (ZVI) composite	138:191	In this study, coarse sand-supported zero valent iron (ZVI) composite was synthesized by adding sodium alginate to immobilize.
26104904	7	43	theme	prime	895:899	arg1	hydrogen					882:889	hydrogen	882:889	hydrogen	882:889	It was concluded that dechlorination was the key reaction pathway, ZVI and hydrogen are prime reductants in dechlorination of 2,4-DCP using ZVI.
26104904	7	43	theme	prime	895:899	arg1	reductants					901:910	prime reductants	895:910	prime reductants in dechlorination of 2,4-DCP using ZVI	895:949	It was concluded that dechlorination was the key reaction pathway, ZVI and hydrogen are prime reductants in dechlorination of 2,4-DCP using ZVI.
26104904	7	43	theme	prime	895:899	arg1	ZVI					874:876	ZVI	874:876	ZVI	874:876	It was concluded that dechlorination was the key reaction pathway, ZVI and hydrogen are prime reductants in dechlorination of 2,4-DCP using ZVI.
25487409	3	0	theme	significant	855:865	arg1	performance					882:892	a significant photocatalytic performance	853:892	a significant photocatalytic performance towards degradation of a methylene blue dye	853:936	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	1	1	theme	ultrathin	225:233	arg1	paper					273:277	an ultrathin titania gel film pre-deposited filter paper	222:277	an ultrathin titania gel film pre-deposited filter paper	222:277	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	2	2	with	formation	674:682	arg1	sizes					727:731	sizes	727:731	sizes of 2-5 nm uniformly anchored on the cellulose nanofibers	727:788	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	3	3	contain	possessing	1066:1075	arg2	activity					1101:1108	excellent antibacterial activity	1077:1108	excellent antibacterial activity against both Gram-positive and Gram-negative bacteria	1077:1162	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	3	3	contain	possessing	1066:1075	arg1	material					1057:1064	an Ag-NP/anatase-titania/cellulose composite material	1012:1064	an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria	1012:1162	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	1	4	theme	titania	235:241	arg1	paper					273:277	an ultrathin titania gel film pre-deposited filter paper	222:277	an ultrathin titania gel film pre-deposited filter paper	222:277	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	2	5	theme	butoxide	451:458	arg1	specie					460:465	The titanium butoxide specie	438:465	The titanium butoxide specie	438:465	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	3	6	theme	excellent	1077:1085	arg1	activity					1101:1108	excellent antibacterial activity	1077:1108	excellent antibacterial activity against both Gram-positive and Gram-negative bacteria	1077:1162	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	2	7	theme	hydrothermal	635:646	arg1	treatment					648:656	the subsequent hydrothermal treatment	620:656	the subsequent hydrothermal treatment	620:656	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	2	8	theme	nanoparticles	708:720	arg1	formation					674:682	the formation	670:682	the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers	670:788	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	2	9	theme	cellulose	769:777	arg1	nanofibers					779:788	the cellulose nanofibers	765:788	the cellulose nanofibers	765:788	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	3	10	theme	Gram-positive	1123:1135	arg1	bacteria					1155:1162	both Gram-positive and Gram-negative bacteria	1118:1162	both Gram-positive and Gram-negative bacteria	1118:1162	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	3	11	theme	composite	989:997	arg1	sheet					999:1003	the composite sheet	985:1003	the composite sheet	985:1003	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	2	12	theme	subsequent	624:633	arg1	treatment					648:656	the subsequent hydrothermal treatment	620:656	the subsequent hydrothermal treatment	620:656	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	1	13	theme	gel	243:245	arg1	paper					273:277	an ultrathin titania gel film pre-deposited filter paper	222:277	an ultrathin titania gel film pre-deposited filter paper	222:277	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	3	14	theme	photocatalytic	867:880	arg1	performance					882:892	a significant photocatalytic performance	853:892	a significant photocatalytic performance towards degradation of a methylene blue dye	853:936	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	1	15	from	nanocrystallites	384:399	arg1	surfaces					428:435	the cellulose nanofiber surfaces	404:435	the cellulose nanofiber surfaces	404:435	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	2	16	theme	solvothermal	544:555	arg1	process					557:563	a solvothermal process	542:563	a solvothermal process	542:563	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	1	17	theme	film	247:250	arg1	paper					273:277	an ultrathin titania gel film pre-deposited filter paper	222:277	an ultrathin titania gel film pre-deposited filter paper	222:277	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	0	18	theme	anatase-titania/cellulose	24:48	arg1	sheet					60:64	Hierarchical-structured anatase-titania/cellulose composite sheet	0:64	Hierarchical-structured anatase-titania/cellulose composite sheet with high photocatalytic performance and antibacterial activity	0:128	Hierarchical-structured anatase-titania/cellulose composite sheet with high photocatalytic performance and antibacterial activity.
25487409	1	19	theme	Bulk	131:134	arg1	sheets					185:190	Bulk hierarchical anatase-titania/cellulose composite sheets	131:190	Bulk hierarchical anatase-titania/cellulose composite sheets	131:190	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	1	20	theme	pre-deposited	252:264	arg1	paper					273:277	an ultrathin titania gel film pre-deposited filter paper	222:277	an ultrathin titania gel film pre-deposited filter paper	222:277	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	0	21	theme	Hierarchical-structured	0:22	arg1	sheet					60:64	Hierarchical-structured anatase-titania/cellulose composite sheet	0:64	Hierarchical-structured anatase-titania/cellulose composite sheet with high photocatalytic performance and antibacterial activity	0:128	Hierarchical-structured anatase-titania/cellulose composite sheet with high photocatalytic performance and antibacterial activity.
25487409	2	22	theme	2-5 nm	736:741	arg1	sizes					727:731	sizes	727:731	sizes of 2-5 nm uniformly anchored on the cellulose nanofibers	727:788	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	1	23	theme	hierarchical	136:147	arg1	sheets					185:190	Bulk hierarchical anatase-titania/cellulose composite sheets	131:190	Bulk hierarchical anatase-titania/cellulose composite sheets	131:190	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	1	24	theme	filter	266:271	arg1	paper					273:277	an ultrathin titania gel film pre-deposited filter paper	222:277	an ultrathin titania gel film pre-deposited filter paper	222:277	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	3	25	theme	blue	929:932	arg1	dye					934:936	a methylene blue dye	917:936	a methylene blue dye	917:936	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	1	26	theme	anatase-titania/cellulose	149:173	arg1	sheets					185:190	Bulk hierarchical anatase-titania/cellulose composite sheets	131:190	Bulk hierarchical anatase-titania/cellulose composite sheets	131:190	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	2	27	theme	substance	524:532	arg1	nanofibers					496:505	the nanofibers	492:505	the nanofibers of the cellulose substance	492:532	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	0	28	theme	composite	50:58	arg1	sheet					60:64	Hierarchical-structured anatase-titania/cellulose composite sheet	0:64	Hierarchical-structured anatase-titania/cellulose composite sheet with high photocatalytic performance and antibacterial activity	0:128	Hierarchical-structured anatase-titania/cellulose composite sheet with high photocatalytic performance and antibacterial activity.
25487409	1	29	theme	anatase-titania	368:382	arg1	nanocrystallites					384:399	anatase-titania nanocrystallites	368:399	anatase-titania nanocrystallites on the cellulose nanofiber surfaces	368:435	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	1	30	theme	composite	175:183	arg1	sheets					185:190	Bulk hierarchical anatase-titania/cellulose composite sheets	131:190	Bulk hierarchical anatase-titania/cellulose composite sheets	131:190	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	3	31	theme	Gram-negative	1141:1153	arg1	bacteria					1155:1162	both Gram-positive and Gram-negative bacteria	1118:1162	both Gram-positive and Gram-negative bacteria	1118:1162	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	2	32	theme	cellulose	514:522	arg1	substance					524:532	the cellulose substance	510:532	the cellulose substance	510:532	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	0	33	theme	high	71:74	arg1	performance					91:101	high photocatalytic performance	71:101	high photocatalytic performance	71:101	Hierarchical-structured anatase-titania/cellulose composite sheet with high photocatalytic performance and antibacterial activity.
25487409	3	34	theme	dye	934:936	arg1	degradation					902:912	degradation	902:912	degradation of a methylene blue dye	902:936	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	0	35	with	sheet	60:64	arg1	activity					121:128	antibacterial activity	107:128	antibacterial activity	107:128	Hierarchical-structured anatase-titania/cellulose composite sheet with high photocatalytic performance and antibacterial activity.
25487409	0	35	with	sheet	60:64	arg1	performance					91:101	high photocatalytic performance	71:101	high photocatalytic performance	71:101	Hierarchical-structured anatase-titania/cellulose composite sheet with high photocatalytic performance and antibacterial activity.
25487409	1	36	theme	solvo-co-hydrothermal	284:304	arg1	treatment					306:314	a solvo-co-hydrothermal treatment	282:314	a solvo-co-hydrothermal treatment	282:314	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	2	37	theme	anatase-titania	692:706	arg1	nanoparticles					708:720	fine anatase-titania nanoparticles	687:720	fine anatase-titania nanoparticles	687:720	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	2	38	theme	titanium	442:449	arg1	specie					460:465	The titanium butoxide specie	438:465	The titanium butoxide specie	438:465	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	0	39	theme	photocatalytic	76:89	arg1	performance					91:101	high photocatalytic performance	71:101	high photocatalytic performance	71:101	Hierarchical-structured anatase-titania/cellulose composite sheet with high photocatalytic performance and antibacterial activity.
25487409	1	40	theme	cellulose	408:416	arg1	surfaces					428:435	the cellulose nanofiber surfaces	404:435	the cellulose nanofiber surfaces	404:435	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	2	41	theme	fine	687:690	arg1	nanoparticles					708:720	fine anatase-titania nanoparticles	687:720	fine anatase-titania nanoparticles	687:720	The titanium butoxide specie is firstly absorbed onto the nanofibers of the cellulose substance through a solvothermal process, which was thereafter hydrolyzed and crystallized upon the subsequent hydrothermal treatment, leading to the formation of fine anatase-titania nanoparticles with sizes of 2-5 nm uniformly anchored on the cellulose nanofibers.
25487409	3	42	theme	anatase-titania/cellulose	805:829	arg1	sheet					841:845	The resulting anatase-titania/cellulose composite sheet	791:845	The resulting anatase-titania/cellulose composite sheet	791:845	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	3	43	theme	composite	1047:1055	arg1	material					1057:1064	an Ag-NP/anatase-titania/cellulose composite material	1012:1064	an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria	1012:1162	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	1	44	theme	nanofiber	418:426	arg1	surfaces					428:435	the cellulose nanofiber surfaces	404:435	the cellulose nanofiber surfaces	404:435	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	3	45	theme	composite	831:839	arg1	sheet					841:845	The resulting anatase-titania/cellulose composite sheet	791:845	The resulting anatase-titania/cellulose composite sheet	791:845	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	3	46	theme	nanoparticles	966:978	arg1	introduction					943:954	introduction	943:954	introduction of silver nanoparticles into the composite sheet	943:1003	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	0	47	theme	antibacterial	107:119	arg1	activity					121:128	antibacterial activity	107:128	antibacterial activity	107:128	Hierarchical-structured anatase-titania/cellulose composite sheet with high photocatalytic performance and antibacterial activity.
25487409	3	48	theme	resulting	795:803	arg1	sheet					841:845	The resulting anatase-titania/cellulose composite sheet	791:845	The resulting anatase-titania/cellulose composite sheet	791:845	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	3	49	theme	Ag-NP/anatase-titania/cellulose	1015:1045	arg1	material					1057:1064	an Ag-NP/anatase-titania/cellulose composite material	1012:1064	an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria	1012:1162	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	3	50	theme	silver	959:964	arg1	nanoparticles					966:978	silver nanoparticles	959:978	silver nanoparticles	959:978	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	3	51	theme	antibacterial	1087:1099	arg1	activity					1101:1108	excellent antibacterial activity	1077:1108	excellent antibacterial activity against both Gram-positive and Gram-negative bacteria	1077:1162	The resulting anatase-titania/cellulose composite sheet shows a significant photocatalytic performance towards degradation of a methylene blue dye, and introduction of silver nanoparticles into the composite sheet yields an Ag-NP/anatase-titania/cellulose composite material possessing excellent antibacterial activity against both Gram-positive and Gram-negative bacteria.
25487409	1	52	theme	titanium	325:332	arg1	butoxide					334:341	titanium butoxide	325:341	titanium butoxide	325:341	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
25487409	1	52	theme	titanium	325:332	arg1	precursor					350:358	the precursor	346:358	the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces	346:435	Bulk hierarchical anatase-titania/cellulose composite sheets were fabricated by subjecting an ultrathin titania gel film pre-deposited filter paper to a solvo-co-hydrothermal treatment by using titanium butoxide as the precursor to grow anatase-titania nanocrystallites on the cellulose nanofiber surfaces.
27180295	5	0	theme	%	673:673	arg1	cells					685:689	% bacterial cells	673:689	% bacterial cells	673:689	% bacterial cells showed that the observed biosorption capacity enhanced by 1.27 times from 398mg/g (CB) to 504mg/g (PICB) that confirmed the effectiveness of cells immobilization process.
27180295	1	1	theme	U	312:312	arg1	biosorption					318:328	U(VI) biosorption	312:328	U(VI) biosorption	312:328	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	0	2	theme	chitosan	60:67	arg1	bead					69:72	bi-functionalized Pseudomonas putida @ chitosan bead	21:72	bi-functionalized Pseudomonas putida @ chitosan bead	21:72	U(VI) biosorption by bi-functionalized Pseudomonas putida @ chitosan bead: Modeling and optimization using RSM.
27180295	2	3	theme	batch	527:531	arg1	mode					533:536	batch mode	527:536	batch mode	527:536	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	7	4	theme	NH2	1083:1085	arg1	groups					1065:1070	the functional groups	1050:1070	the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g)	1050:1107	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	4	theme	NH2	1083:1085	arg1	NH2					1083:1085	NH2	1083:1085	NH2	1083:1085	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	4	theme	NH2	1083:1085	arg1	OH					1088:1089	OH	1088:1089	OH	1088:1089	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	4	theme	NH2	1083:1085	arg1	NH3					1075:1077	NH3	1075:1077	NH3	1075:1077	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	4	theme	NH2	1083:1085	arg1	responsible					1114:1124	responsible	1114:1124	responsible	1114:1124	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	4	theme	NH2	1083:1085	arg1	COOH					1092:1095	COOH	1092:1095	COOH (6.00meq/g)	1092:1107	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	5	theme	binding	1000:1006	arg1	process					1008:1014	the binding process	996:1014	the binding process	996:1014	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	1	6	from	capacity	276:283	arg1	solutions					343:351	aqueous solutions	335:351	aqueous solutions	335:351	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	5	7	theme	process	853:859	arg1	effectiveness					815:827	the effectiveness	811:827	the effectiveness of cells immobilization process	811:859	% bacterial cells showed that the observed biosorption capacity enhanced by 1.27 times from 398mg/g (CB) to 504mg/g (PICB) that confirmed the effectiveness of cells immobilization process.
27180295	5	8	theme	immobilization	838:851	arg1	process					853:859	cells immobilization process	832:859	cells immobilization process	832:859	% bacterial cells showed that the observed biosorption capacity enhanced by 1.27 times from 398mg/g (CB) to 504mg/g (PICB) that confirmed the effectiveness of cells immobilization process.
27180295	5	9	theme	biosorption	716:726	arg1	capacity					728:735	the observed biosorption capacity	703:735	the observed biosorption capacity	703:735	% bacterial cells showed that the observed biosorption capacity enhanced by 1.27 times from 398mg/g (CB) to 504mg/g (PICB) that confirmed the effectiveness of cells immobilization process.
27180295	8	10	theme	equilibrium	1144:1154	arg1	studies					1168:1174	The equilibrium and kinetic studies	1140:1174	studies	1168:1174	The equilibrium and kinetic studies revealed that the Langmuir isotherm model and the pseudo-second-order kinetic model were in better fitness with the CB and PICB experimental data.
27180295	6	11	theme	potentiometric	871:884	arg1	titration					886:894	potentiometric titration	871:894	potentiometric titration	871:894	FTIR and potentiometric titration were then utilized to characterize the prepared biosorbents.
27180295	1	12	from	solutions	343:351	arg1	capacity					276:283	biosorption capacity	264:283	biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions	264:351	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	1	12	from	solutions	343:351	arg1	biosorbent					297:306	prepared biosorbent	288:306	prepared biosorbent for U(VI) biosorption from aqueous solutions	288:351	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	6	13	used	utilized	906:913	arg2	FTIR					862:865	FTIR	862:865	FTIR	862:865	FTIR and potentiometric titration were then utilized to characterize the prepared biosorbents.
27180295	6	13	used	utilized	906:913	arg2	titration					886:894	potentiometric titration	871:894	potentiometric titration	871:894	FTIR and potentiometric titration were then utilized to characterize the prepared biosorbents.
27180295	5	14	theme	observed	707:714	arg1	capacity					728:735	the observed biosorption capacity	703:735	the observed biosorption capacity	703:735	% bacterial cells showed that the observed biosorption capacity enhanced by 1.27 times from 398mg/g (CB) to 504mg/g (PICB) that confirmed the effectiveness of cells immobilization process.
27180295	1	15	theme	putida	138:143	arg1	cells					145:149	Pseudomonas putida cells	126:149	Pseudomonas putida cells immobilized into chitosan beads (PICB)	126:188	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	7	16	theme	functional	1054:1063	arg1	groups					1065:1070	the functional groups	1050:1070	the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g)	1050:1107	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	16	theme	functional	1054:1063	arg1	NH2					1083:1085	NH2	1083:1085	NH2	1083:1085	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	16	theme	functional	1054:1063	arg1	OH					1088:1089	OH	1088:1089	OH	1088:1089	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	16	theme	functional	1054:1063	arg1	NH3					1075:1077	NH3	1075:1077	NH3	1075:1077	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	16	theme	functional	1054:1063	arg1	responsible					1114:1124	responsible	1114:1124	responsible	1114:1124	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	16	theme	functional	1054:1063	arg1	COOH					1092:1095	COOH	1092:1095	COOH (6.00meq/g)	1092:1107	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	8	17	theme	kinetic	1160:1166	arg1	studies					1168:1174	The equilibrium and kinetic studies	1140:1174	studies	1168:1174	The equilibrium and kinetic studies revealed that the Langmuir isotherm model and the pseudo-second-order kinetic model were in better fitness with the CB and PICB experimental data.
27180295	9	18	dep	biosorption	1428:1438	arg1	VI					1424:1425	VI	1424:1425	VI	1424:1425	In conclusion, the present study indicated that the PICB could be a suitable biosorbent for uranium (VI) biosorption from aqueous solutions.
27180295	2	19	theme	Central	398:404	arg1	CCD					424:426	CCD	424:426	CCD	424:426	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	2	19	theme	Central	398:404	arg1	Design					416:421	Central Composite Design	398:421	Central Composite Design (CCD)	398:427	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	7	20	from	group	987:991	arg1	process					1008:1014	the binding process	996:1014	the binding process	996:1014	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	2	21	theme	Response	354:361	arg1	RSM					384:386	RSM	384:386	RSM	384:386	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	2	21	theme	Response	354:361	arg1	Methodology					371:381	Response Surface Methodology	354:381	Response Surface Methodology (RSM) based on Central Composite Design (CCD)	354:427	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	2	22	theme	Composite	406:414	arg1	CCD					424:426	CCD	424:426	CCD	424:426	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	2	22	theme	Composite	406:414	arg1	Design					416:421	Central Composite Design	398:421	Central Composite Design (CCD)	398:427	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	1	23	theme	aqueous	335:341	arg1	solutions					343:351	aqueous solutions	335:351	aqueous solutions	335:351	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	4	24	theme	dosage	650:655	arg1	0.4g/L					657:662	biosorbent dosage 0.4g/L	639:662	biosorbent dosage 0.4g/L	639:662	pH 5, initial U(VI) concentration 500mg/L, biosorbent dosage 0.4g/L and 20wt.
27180295	0	25	theme	U	0:0	arg1	biosorption					6:16	U(VI) biosorption	0:16	U(VI) biosorption by bi-functionalized Pseudomonas putida @ chitosan bead: Modeling and optimization using RSM.	0:110	U(VI) biosorption by bi-functionalized Pseudomonas putida @ chitosan bead: Modeling and optimization using RSM.
27180295	1	26	theme	microorganism	236:248	arg1	entrapment					250:259	microorganism entrapment	236:259	microorganism entrapment	236:259	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	7	27	theme	NH3	1075:1077	arg1	groups					1065:1070	the functional groups	1050:1070	the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g)	1050:1107	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	27	theme	NH3	1075:1077	arg1	NH2					1083:1085	NH2	1083:1085	NH2	1083:1085	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	27	theme	NH3	1075:1077	arg1	OH					1088:1089	OH	1088:1089	OH	1088:1089	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	27	theme	NH3	1075:1077	arg1	NH3					1075:1077	NH3	1075:1077	NH3	1075:1077	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	27	theme	NH3	1075:1077	arg1	responsible					1114:1124	responsible	1114:1124	responsible	1114:1124	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	27	theme	NH3	1075:1077	arg1	COOH					1092:1095	COOH	1092:1095	COOH (6.00meq/g)	1092:1107	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	28	theme	dominant	967:974	arg1	NH3					1020:1022	NH3	1020:1022	NH3	1020:1022	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	28	theme	dominant	967:974	arg1	group					987:991	the dominant functional group	963:991	the dominant functional group in the binding process	963:1014	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	4	29	theme	biosorbent	639:648	arg1	0.4g/L					657:662	biosorbent dosage 0.4g/L	639:662	biosorbent dosage 0.4g/L	639:662	pH 5, initial U(VI) concentration 500mg/L, biosorbent dosage 0.4g/L and 20wt.
27180295	9	30	theme	aqueous	1445:1451	arg1	solutions					1453:1461	aqueous solutions	1445:1461	aqueous solutions	1445:1461	In conclusion, the present study indicated that the PICB could be a suitable biosorbent for uranium (VI) biosorption from aqueous solutions.
27180295	1	31	theme	entrapment	250:259	arg1	impact					226:231	the impact	222:231	the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions	222:351	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	1	32	from	impact	226:231	arg1	capacity					276:283	biosorption capacity	264:283	biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions	264:351	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	9	33	theme	uranium	1415:1421	arg1	biosorption					1428:1438	uranium (VI) biosorption	1415:1438	uranium (VI) biosorption	1415:1438	In conclusion, the present study indicated that the PICB could be a suitable biosorbent for uranium (VI) biosorption from aqueous solutions.
27180295	5	34	theme	cells	832:836	arg1	process					853:859	cells immobilization process	832:859	cells immobilization process	832:859	% bacterial cells showed that the observed biosorption capacity enhanced by 1.27 times from 398mg/g (CB) to 504mg/g (PICB) that confirmed the effectiveness of cells immobilization process.
27180295	1	35	theme	chitosan	168:175	arg1	beads					177:181	chitosan beads	168:181	chitosan beads (PICB)	168:188	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	1	35	theme	chitosan	168:175	arg1	PICB					184:187	PICB	184:187	PICB	184:187	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	4	36	dep	500mg/L	630:636	arg1	VI					612:613	VI	612:613	VI	612:613	pH 5, initial U(VI) concentration 500mg/L, biosorbent dosage 0.4g/L and 20wt.
27180295	8	37	theme	better	1268:1273	arg1	fitness					1275:1281	better fitness	1268:1281	better fitness with the CB and PICB experimental data	1268:1320	The equilibrium and kinetic studies revealed that the Langmuir isotherm model and the pseudo-second-order kinetic model were in better fitness with the CB and PICB experimental data.
27180295	0	38	dep	putida	51:56	arg1	Pseudomonas					39:49	Pseudomonas	39:49	Pseudomonas	39:49	U(VI) biosorption by bi-functionalized Pseudomonas putida @ chitosan bead: Modeling and optimization using RSM.
27180295	9	39	theme	present	1342:1348	arg1	study					1350:1354	the present study	1338:1354	the present study	1338:1354	In conclusion, the present study indicated that the PICB could be a suitable biosorbent for uranium (VI) biosorption from aqueous solutions.
27180295	3	40	dep	Performing	539:548	arg1	viz					591:593	viz	591:593	viz	591:593	Performing experiments under optimal condition sets viz.
27180295	1	41	theme	biosorption	264:274	arg1	capacity					276:283	biosorption capacity	264:283	biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions	264:351	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	9	42	theme	suitable	1391:1398	arg1	biosorbent					1400:1409	a suitable biosorbent	1389:1409	a suitable biosorbent for uranium (VI) biosorption from aqueous solutions	1389:1461	In conclusion, the present study indicated that the PICB could be a suitable biosorbent for uranium (VI) biosorption from aqueous solutions.
27180295	9	42	theme	suitable	1391:1398	arg1	PICB					1375:1378	the PICB	1371:1378	the PICB	1371:1378	In conclusion, the present study indicated that the PICB could be a suitable biosorbent for uranium (VI) biosorption from aqueous solutions.
27180295	8	43	theme	experimental	1304:1315	arg1	data					1317:1320	the CB and PICB experimental data	1288:1320	data	1317:1320	The equilibrium and kinetic studies revealed that the Langmuir isotherm model and the pseudo-second-order kinetic model were in better fitness with the CB and PICB experimental data.
27180295	2	44	theme	Surface	363:369	arg1	RSM					384:386	RSM	384:386	RSM	384:386	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	2	44	theme	Surface	363:369	arg1	Methodology					371:381	Response Surface Methodology	354:381	Response Surface Methodology (RSM) based on Central Composite Design (CCD)	354:427	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	9	45	from	solutions	1453:1461	arg1	biosorbent					1400:1409	a suitable biosorbent	1389:1409	a suitable biosorbent for uranium (VI) biosorption from aqueous solutions	1389:1461	In conclusion, the present study indicated that the PICB could be a suitable biosorbent for uranium (VI) biosorption from aqueous solutions.
27180295	9	45	from	solutions	1453:1461	arg1	PICB					1375:1378	the PICB	1371:1378	the PICB	1371:1378	In conclusion, the present study indicated that the PICB could be a suitable biosorbent for uranium (VI) biosorption from aqueous solutions.
27180295	2	46	theme	PICB	477:480	arg1	performance					458:468	the performance	454:468	the performance of the PICB in comparison with chitosan beads (CB) under batch mode	454:536	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	3	47	theme	optimal	568:574	arg1	sets					586:589	optimal condition sets	568:589	optimal condition sets	568:589	Performing experiments under optimal condition sets viz.
27180295	8	48	theme	isotherm	1203:1210	arg1	model					1212:1216	the Langmuir isotherm model	1190:1216	the Langmuir isotherm model	1190:1216	The equilibrium and kinetic studies revealed that the Langmuir isotherm model and the pseudo-second-order kinetic model were in better fitness with the CB and PICB experimental data.
27180295	1	49	theme	biosorbent	297:306	arg1	capacity					276:283	biosorption capacity	264:283	biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions	264:351	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	7	50	from	NH3	1020:1022	arg1	CB					1046:1047	the CB	1042:1047	the CB	1042:1047	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	3	51	theme	condition	576:584	arg1	sets					586:589	optimal condition sets	568:589	optimal condition sets	568:589	Performing experiments under optimal condition sets viz.
27180295	1	52	dep	putida	138:143	arg1	Pseudomonas					126:136	Pseudomonas	126:136	Pseudomonas	126:136	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	4	53	theme	concentration	616:628	arg1	500mg/L					630:636	initial U(VI) concentration 500mg/L	602:636	initial U(VI) concentration 500mg/L	602:636	pH 5, initial U(VI) concentration 500mg/L, biosorbent dosage 0.4g/L and 20wt.
27180295	0	54	theme	bi-functionalized	21:37	arg1	bead					69:72	bi-functionalized Pseudomonas putida @ chitosan bead	21:72	bi-functionalized Pseudomonas putida @ chitosan bead	21:72	U(VI) biosorption by bi-functionalized Pseudomonas putida @ chitosan bead: Modeling and optimization using RSM.
27180295	1	55	theme	prepared	288:295	arg1	biosorbent					297:306	prepared biosorbent	288:306	prepared biosorbent for U(VI) biosorption from aqueous solutions	288:351	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	7	56	theme	functional	976:985	arg1	NH3					1020:1022	NH3	1020:1022	NH3	1020:1022	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	56	theme	functional	976:985	arg1	group					987:991	the dominant functional group	963:991	the dominant functional group in the binding process	963:1014	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	2	57	from	performance	458:468	arg1	comparison					485:494	comparison	485:494	comparison with chitosan beads (CB) under batch mode	485:536	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	0	58	dep	biosorption	6:16	arg1	VI					2:3	VI	2:3	VI	2:3	U(VI) biosorption by bi-functionalized Pseudomonas putida @ chitosan bead: Modeling and optimization using RSM.
27180295	0	58	dep	biosorption	6:16	arg1	Modeling					75:82	Modeling	75:82	Modeling	75:82	U(VI) biosorption by bi-functionalized Pseudomonas putida @ chitosan bead: Modeling and optimization using RSM.
27180295	0	58	dep	biosorption	6:16	arg1	optimization					88:99	optimization	88:99	optimization	88:99	U(VI) biosorption by bi-functionalized Pseudomonas putida @ chitosan bead: Modeling and optimization using RSM.
27180295	8	59	with	fitness	1275:1281	arg1	CB					1292:1293	the CB and PICB experimental data	1288:1320	CB	1292:1293	The equilibrium and kinetic studies revealed that the Langmuir isotherm model and the pseudo-second-order kinetic model were in better fitness with the CB and PICB experimental data.
27180295	8	59	with	fitness	1275:1281	arg1	data					1317:1320	the CB and PICB experimental data	1288:1320	data	1317:1320	The equilibrium and kinetic studies revealed that the Langmuir isotherm model and the pseudo-second-order kinetic model were in better fitness with the CB and PICB experimental data.
27180295	1	60	dep	biosorption	318:328	arg1	VI					314:315	VI	314:315	VI	314:315	In this work, Pseudomonas putida cells immobilized into chitosan beads (PICB) were synthesized to investigate the impact of microorganism entrapment on biosorption capacity of prepared biosorbent for U(VI) biosorption from aqueous solutions.
27180295	2	61	used	utilized	433:440	arg2	RSM					384:386	RSM	384:386	RSM	384:386	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	2	61	used	utilized	433:440	arg2	Methodology					371:381	Response Surface Methodology	354:381	Response Surface Methodology (RSM) based on Central Composite Design (CCD)	354:427	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	8	62	theme	kinetic	1246:1252	arg1	model					1254:1258	the pseudo-second-order kinetic model	1222:1258	the pseudo-second-order kinetic model	1222:1258	The equilibrium and kinetic studies revealed that the Langmuir isotherm model and the pseudo-second-order kinetic model were in better fitness with the CB and PICB experimental data.
27180295	8	63	theme	Langmuir	1194:1201	arg1	model					1212:1216	the Langmuir isotherm model	1190:1216	the Langmuir isotherm model	1190:1216	The equilibrium and kinetic studies revealed that the Langmuir isotherm model and the pseudo-second-order kinetic model were in better fitness with the CB and PICB experimental data.
27180295	2	64	theme	chitosan	501:508	arg1	beads					510:514	chitosan beads	501:514	chitosan beads (CB) under batch mode	501:536	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	2	64	theme	chitosan	501:508	arg1	CB					517:518	CB	517:518	CB	517:518	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	8	65	theme	PICB	1299:1302	arg1	data					1317:1320	the CB and PICB experimental data	1288:1320	data	1317:1320	The equilibrium and kinetic studies revealed that the Langmuir isotherm model and the pseudo-second-order kinetic model were in better fitness with the CB and PICB experimental data.
27180295	8	66	theme	pseudo-second-order	1226:1244	arg1	model					1254:1258	the pseudo-second-order kinetic model	1222:1258	the pseudo-second-order kinetic model	1222:1258	The equilibrium and kinetic studies revealed that the Langmuir isotherm model and the pseudo-second-order kinetic model were in better fitness with the CB and PICB experimental data.
27180295	5	67	theme	bacterial	675:683	arg1	cells					685:689	% bacterial cells	673:689	% bacterial cells	673:689	% bacterial cells showed that the observed biosorption capacity enhanced by 1.27 times from 398mg/g (CB) to 504mg/g (PICB) that confirmed the effectiveness of cells immobilization process.
27180295	7	68	theme	OH	1088:1089	arg1	groups					1065:1070	the functional groups	1050:1070	the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g)	1050:1107	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	68	theme	OH	1088:1089	arg1	NH2					1083:1085	NH2	1083:1085	NH2	1083:1085	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	68	theme	OH	1088:1089	arg1	OH					1088:1089	OH	1088:1089	OH	1088:1089	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	68	theme	OH	1088:1089	arg1	NH3					1075:1077	NH3	1075:1077	NH3	1075:1077	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	68	theme	OH	1088:1089	arg1	responsible					1114:1124	responsible	1114:1124	responsible	1114:1124	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	7	68	theme	OH	1088:1089	arg1	COOH					1092:1095	COOH	1092:1095	COOH (6.00meq/g)	1092:1107	While the dominant functional group in the binding process was NH3(+) (4.78meq/g) in the CB, the functional groups of NH3(+), NH2, OH, COOH (6.00meq/g) were responsible for the PICB.
27180295	0	69	theme	@	58:58	arg1	bead					69:72	bi-functionalized Pseudomonas putida @ chitosan bead	21:72	bi-functionalized Pseudomonas putida @ chitosan bead	21:72	U(VI) biosorption by bi-functionalized Pseudomonas putida @ chitosan bead: Modeling and optimization using RSM.
27180295	6	70	theme	prepared	935:942	arg1	biosorbents					944:954	the prepared biosorbents	931:954	the prepared biosorbents	931:954	FTIR and potentiometric titration were then utilized to characterize the prepared biosorbents.
27180295	4	71	theme	U	610:610	arg1	500mg/L					630:636	initial U(VI) concentration 500mg/L	602:636	initial U(VI) concentration 500mg/L	602:636	pH 5, initial U(VI) concentration 500mg/L, biosorbent dosage 0.4g/L and 20wt.
27180295	2	72	with	comparison	485:494	arg1	beads					510:514	chitosan beads	501:514	chitosan beads (CB) under batch mode	501:536	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	2	72	with	comparison	485:494	arg1	CB					517:518	CB	517:518	CB	517:518	Response Surface Methodology (RSM) based on Central Composite Design (CCD) was utilized to evaluate the performance of the PICB in comparison with chitosan beads (CB) under batch mode.
27180295	0	73	theme	putida	51:56	arg1	bead					69:72	bi-functionalized Pseudomonas putida @ chitosan bead	21:72	bi-functionalized Pseudomonas putida @ chitosan bead	21:72	U(VI) biosorption by bi-functionalized Pseudomonas putida @ chitosan bead: Modeling and optimization using RSM.
27180295	5	74	from	398mg/g	765:771	arg1	times					754:758	1.27 times	749:758	1.27 times from 398mg/g (CB)	749:776	% bacterial cells showed that the observed biosorption capacity enhanced by 1.27 times from 398mg/g (CB) to 504mg/g (PICB) that confirmed the effectiveness of cells immobilization process.
27180295	4	75	theme	initial	602:608	arg1	500mg/L					630:636	initial U(VI) concentration 500mg/L	602:636	initial U(VI) concentration 500mg/L	602:636	pH 5, initial U(VI) concentration 500mg/L, biosorbent dosage 0.4g/L and 20wt.
26593602	0	0	theme	external	74:81	arg1	L.					70:71	Cynara scolymus L.	54:71	Cynara scolymus L.	54:71	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	0	0	theme	external	74:81	arg1	bracts					83:88	artichoke (Cynara scolymus L.) external bracts	43:88	artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction	43:158	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	1	1	theme	Microwave	161:169	arg1	MAE					192:194	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods	161:243	MAE	192:194	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	2	2	theme	higher	578:583	arg1	concentrations					585:598	slightly higher concentrations	569:598	slightly higher concentrations of inositol	569:610	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	2	3	theme	g	531:531	arg1	min					520:522	3 min	518:522	3 min of 0.3 g of sample	518:541	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	1	4	theme	bioactive	401:409	arg1	inulin					440:445	inulin	440:445	inulin	440:445	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	1	4	theme	bioactive	401:409	arg1	inositols					426:434	inositols	426:434	inositols	426:434	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	1	4	theme	bioactive	401:409	arg1	carbohydrates					411:423	bioactive carbohydrates	401:423	bioactive carbohydrates (inositols and inulin)	401:446	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	1	5	theme	assisted	171:178	arg1	MAE					192:194	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods	161:243	MAE	192:194	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	2	6	from	°C	511:512	arg1	MAE					501:503	MAE	501:503	MAE at 60 °C for 3 min of 0.3 g of sample	501:541	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	1	7	theme	carbohydrates	411:423	arg1	extraction					387:396	the effective extraction	373:396	the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts	373:498	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	3	8	theme	inulin	757:762	arg1	concentrations					739:752	higher concentrations	732:752	higher concentrations of inulin	732:762	On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques.
26593602	3	9	theme	successive	860:869	arg1	cycles					882:887	two successive extraction cycles	856:887	two successive extraction cycles for both techniques	856:907	On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques.
26593602	4	10	theme	bioactive	1000:1008	arg1	carbohydrates					1010:1022	these bioactive carbohydrates	994:1022	these bioactive carbohydrates	994:1022	Both methodologies can be considered appropriate for the simultaneous extraction of these bioactive carbohydrates from this particular industrial by-product.
26593602	1	11	dep	Cynara	464:469	arg1	L.					480:481	Cynara scolymus L.	464:481	Cynara scolymus L.	464:481	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	0	12	theme	assisted	106:113	arg1	extraction					115:124	microwave assisted extraction	96:124	microwave assisted extraction	96:124	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	1	13	theme	composite	323:331	arg1	designs					346:352	3(2) composite experimental designs	318:352	3(2) composite experimental designs	318:352	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	3	14	theme	extraction	871:880	arg1	cycles					882:887	two successive extraction cycles	856:887	two successive extraction cycles for both techniques	856:907	On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques.
26593602	3	15	dep	mg/g	826:829	arg1	sample					835:840	dry sample	831:840	dry sample	831:840	On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques.
26593602	1	16	theme	extraction	180:189	arg1	MAE					192:194	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods	161:243	MAE	192:194	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	0	17	theme	microwave	96:104	arg1	extraction					115:124	microwave assisted extraction	96:124	microwave assisted extraction	96:124	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	1	18	theme	experimental	333:344	arg1	designs					346:352	3(2) composite experimental designs	318:352	3(2) composite experimental designs	318:352	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	1	19	dep	artichoke	453:461	arg1	Cynara					464:469	Cynara scolymus L.	464:481	Cynara scolymus L.	464:481	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	1	20	from	bracts	493:498	arg1	extraction					387:396	the effective extraction	373:396	the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts	373:498	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	2	21	theme	concentrations	585:598	arg1	extraction					555:564	the extraction	551:564	the extraction of slightly higher concentrations of inositol than PLE	551:619	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	2	22	theme	mg/g	649:652	arg1	sample					658:663	11.6 mg/g dry sample	644:663	11.6 mg/g dry sample	644:663	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	2	23	theme	sample	536:541	arg1	g					531:531	0.3 g	527:531	0.3 g of sample	527:541	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	2	24	theme	inositol	603:610	arg1	concentrations					585:598	slightly higher concentrations	569:598	slightly higher concentrations of inositol	569:610	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	3	25	theme	mg/g	812:815	arg1	technique					795:803	the latter technique	784:803	the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample)	784:841	On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques.
26593602	3	25	theme	mg/g	812:815	arg1	mg/g					826:829	185.4 mg/g vs. 96.4 mg/g dry sample	806:840	mg/g	826:829	On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques.
26593602	0	26	theme	carbohydrates	24:36	arg1	Extraction					0:9	Extraction	0:9	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.	0:159	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	2	27	theme	dry	678:680	arg1	sample					682:687	7.6 mg/g dry sample	669:687	7.6 mg/g dry sample	669:687	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	0	28	theme	bioactive	14:22	arg1	carbohydrates					24:36	bioactive carbohydrates	14:36	bioactive carbohydrates	14:36	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	3	29	theme	latter	788:793	arg1	96.4					821:824	96.4	821:824	96.4	821:824	On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques.
26593602	3	29	theme	latter	788:793	arg1	technique					795:803	the latter technique	784:803	the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample)	784:841	On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques.
26593602	3	29	theme	latter	788:793	arg1	mg/g					826:829	185.4 mg/g vs. 96.4 mg/g dry sample	806:840	mg/g	826:829	On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques.
26593602	2	30	theme	mg/g	673:676	arg1	sample					682:687	7.6 mg/g dry sample	669:687	7.6 mg/g dry sample	669:687	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	1	31	theme	pressurized	201:211	arg1	methods					237:243	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods	161:243	methods	237:243	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	0	32	theme	artichoke	43:51	arg1	L.					70:71	Cynara scolymus L.	54:71	Cynara scolymus L.	54:71	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	0	32	theme	artichoke	43:51	arg1	bracts					83:88	artichoke (Cynara scolymus L.) external bracts	43:88	artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction	43:158	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	4	33	theme	carbohydrates	1010:1022	arg1	extraction					980:989	the simultaneous extraction	963:989	the simultaneous extraction of these bioactive carbohydrates from this particular industrial by-product	963:1065	Both methodologies can be considered appropriate for the simultaneous extraction of these bioactive carbohydrates from this particular industrial by-product.
26593602	0	34	theme	liquid	142:147	arg1	extraction					149:158	pressurized liquid extraction	130:158	pressurized liquid extraction	130:158	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	2	35	theme	7.6	669:671	arg1	mg/g					673:676	mg/g	673:676	mg/g	673:676	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	1	36	theme	liquid	213:218	arg1	methods					237:243	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods	161:243	methods	237:243	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	0	37	theme	pressurized	130:140	arg1	extraction					149:158	pressurized liquid extraction	130:158	pressurized liquid extraction	130:158	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	1	38	theme	artichoke	453:461	arg1	bracts					493:498	artichoke (Cynara scolymus L.) external bracts	453:498	artichoke (Cynara scolymus L.) external bracts	453:498	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	1	39	theme	extraction	220:229	arg1	methods					237:243	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods	161:243	methods	237:243	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	0	40	theme	Cynara	54:59	arg1	L.					70:71	Cynara scolymus L.	54:71	Cynara scolymus L.	54:71	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	0	40	theme	Cynara	54:59	arg1	bracts					83:88	artichoke (Cynara scolymus L.) external bracts	43:88	artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction	43:158	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	5	41	dep	time	1115:1118	arg1	applied					1146:1152	applied	1146:1152	are applied for this purpose	1142:1169	To the best of our knowledge this is the first time that these techniques are applied for this purpose.
26593602	3	42	theme	higher	732:737	arg1	concentrations					739:752	higher concentrations	732:752	higher concentrations of inulin	732:762	On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques.
26593602	1	43	theme	effective	377:385	arg1	extraction					387:396	the effective extraction	373:396	the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts	373:498	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	2	44	dep	min	639:641	arg1	sample					658:663	11.6 mg/g dry sample	644:663	11.6 mg/g dry sample	644:663	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	2	44	dep	min	639:641	arg1	sample					682:687	7.6 mg/g dry sample	669:687	7.6 mg/g dry sample	669:687	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	1	45	theme	scolymus	471:478	arg1	Cynara					464:469	Cynara scolymus L.	464:481	Cynara scolymus L.	464:481	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	5	46	theme	first	1109:1113	arg1	time					1115:1118	the first time	1105:1118	the first time that these techniques are applied for this purpose	1105:1169	To the best of our knowledge this is the first time that these techniques are applied for this purpose.
26593602	5	46	theme	first	1109:1113	arg1	this					1097:1100	this	1097:1100	this	1097:1100	To the best of our knowledge this is the first time that these techniques are applied for this purpose.
26593602	3	47	theme	dry	831:833	arg1	sample					835:840	dry sample	831:840	dry sample	831:840	On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques.
26593602	2	48	theme	11.6	644:647	arg1	mg/g					649:652	mg/g	649:652	mg/g	649:652	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
26593602	4	49	theme	particular	1034:1043	arg1	by-product					1056:1065	this particular industrial by-product	1029:1065	this particular industrial by-product	1029:1065	Both methodologies can be considered appropriate for the simultaneous extraction of these bioactive carbohydrates from this particular industrial by-product.
26593602	4	50	theme	simultaneous	967:978	arg1	extraction					980:989	the simultaneous extraction	963:989	the simultaneous extraction of these bioactive carbohydrates from this particular industrial by-product	963:1065	Both methodologies can be considered appropriate for the simultaneous extraction of these bioactive carbohydrates from this particular industrial by-product.
26593602	1	51	theme	PLE	232:234	arg1	methods					237:243	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods	161:243	methods	237:243	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	0	52	from	bracts	83:88	arg1	Extraction					0:9	Extraction	0:9	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.	0:159	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	4	53	from	by-product	1056:1065	arg1	extraction					980:989	the simultaneous extraction	963:989	the simultaneous extraction of these bioactive carbohydrates from this particular industrial by-product	963:1065	Both methodologies can be considered appropriate for the simultaneous extraction of these bioactive carbohydrates from this particular industrial by-product.
26593602	4	54	theme	industrial	1045:1054	arg1	by-product					1056:1065	this particular industrial by-product	1029:1065	this particular industrial by-product	1029:1065	Both methodologies can be considered appropriate for the simultaneous extraction of these bioactive carbohydrates from this particular industrial by-product.
26593602	1	55	dep	carbohydrates	411:423	arg1	inulin					440:445	inulin	440:445	inulin	440:445	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	1	55	dep	carbohydrates	411:423	arg1	inositols					426:434	inositols	426:434	inositols	426:434	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	1	55	dep	carbohydrates	411:423	arg1	carbohydrates					411:423	bioactive carbohydrates	401:423	bioactive carbohydrates (inositols and inulin)	401:446	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	0	56	theme	scolymus	61:68	arg1	L.					70:71	Cynara scolymus L.	54:71	Cynara scolymus L.	54:71	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	0	56	theme	scolymus	61:68	arg1	bracts					83:88	artichoke (Cynara scolymus L.) external bracts	43:88	artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction	43:158	Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.
26593602	1	57	theme	external	484:491	arg1	bracts					493:498	artichoke (Cynara scolymus L.) external bracts	453:498	artichoke (Cynara scolymus L.) external bracts	453:498	Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts.
26593602	2	58	theme	dry	654:656	arg1	sample					658:663	11.6 mg/g dry sample	644:663	11.6 mg/g dry sample	644:663	MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample).
29054658	3	0	theme	sediment	688:695	arg1	cores					697:701	The vadose zone sediment cores	672:701	The vadose zone sediment cores	672:701	The vadose zone sediment cores were drilled from a depth of 11.3-14.6m in an herbicide-contaminated groundwater area.
29054658	1	1	from	soil	177:180	arg1	threat					222:227	a global threat	213:227	a global threat for drinking water safety	213:253	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
29054658	1	1	from	soil	177:180	arg1	leaching					163:170	Pesticides leaching	152:170	Pesticides leaching from soil to surface and groundwater	152:207	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
29054658	1	2	theme	Pesticides	152:161	arg1	threat					222:227	a global threat	213:227	a global threat for drinking water safety	213:253	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
29054658	1	2	theme	Pesticides	152:161	arg1	leaching					163:170	Pesticides leaching	152:170	Pesticides leaching from soil to surface and groundwater	152:207	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
29054658	8	3	from	bioavailability	1404:1418	arg1	phase					1445:1449	the water phase	1435:1449	the water phase	1435:1449	The bioavailability of atrazine in the water phase seemed to be limited, which could be due to complex formation with organic and inorganic colloids.
29054658	1	4	theme	water	242:246	arg1	safety					248:253	drinking water safety	233:253	drinking water safety	233:253	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
29054658	0	5	theme	microbial	60:68	arg1	herbicide					83:91	herbicide	83:91	herbicide	83:91	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	2	6	with	NH4NO3	373:378	arg1	Na-citrate					409:418	Na-citrate	409:418	Na-citrate	409:418	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	6	with	NH4NO3	373:378	arg1	citrate					400:406	sodium citrate	393:406	sodium citrate (Na-citrate)	393:419	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	7	from	hexazinone	501:510	arg1	slurries					597:604	sediment slurries	588:604	sediment slurries	588:604	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	8	with	additions	463:471	arg1	Na-citrate					409:418	Na-citrate	409:418	Na-citrate	409:418	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	8	with	additions	463:471	arg1	citrate					400:406	sodium citrate	393:406	sodium citrate (Na-citrate)	393:419	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	9	9	theme	Atrazine	1550:1557	arg1	degradation					1559:1569	Atrazine degradation	1550:1569	Atrazine degradation by indigenous microbes	1550:1592	Atrazine degradation by indigenous microbes could not be stimulated by the surfactant methyl-β-cyclodextrin, or by the additives NH4NO3 and NH4NO3 with Na-citrate, although the nitrogen additives increased microbial growth.
29054658	8	10	theme	organic	1518:1524	arg1	colloids					1540:1547	organic and inorganic colloids	1518:1547	organic and inorganic colloids	1518:1547	The bioavailability of atrazine in the water phase seemed to be limited, which could be due to complex formation with organic and inorganic colloids.
29054658	6	11	dep	observed	1258:1265	arg1	not					1205:1207	not	1205:1207	not	1205:1207	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	0	12	from	Influence	0:8	arg1	herbicide					83:91	herbicide	83:91	herbicide	83:91	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	0	12	from	Influence	0:8	arg1	degradate					97:105	degradate	97:105	degradate	97:105	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	8	13	theme	complex	1495:1501	arg1	formation					1503:1511	complex formation	1495:1511	complex formation with organic and inorganic colloids	1495:1547	The bioavailability of atrazine in the water phase seemed to be limited, which could be due to complex formation with organic and inorganic colloids.
29054658	9	14	theme	indigenous	1574:1583	arg1	microbes					1585:1592	indigenous microbes	1574:1592	indigenous microbes	1574:1592	Atrazine degradation by indigenous microbes could not be stimulated by the surfactant methyl-β-cyclodextrin, or by the additives NH4NO3 and NH4NO3 with Na-citrate, although the nitrogen additives increased microbial growth.
29054658	0	15	theme	chemical	74:81	arg1	herbicide					83:91	herbicide	83:91	herbicide	83:91	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	2	16	with	NH4NO3	381:386	arg1	Na-citrate					409:418	Na-citrate	409:418	Na-citrate	409:418	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	16	with	NH4NO3	381:386	arg1	citrate					400:406	sodium citrate	393:406	sodium citrate (Na-citrate)	393:419	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	17	theme	BAM	567:569	arg1	dissipations					572:583	the degradate 2,6-dichlorobenzamide (BAM) dissipations	530:583	the degradate 2,6-dichlorobenzamide (BAM) dissipations	530:583	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	18	theme	anaerobic	624:632	arg1	conditions					634:643	aerobic and anaerobic conditions	612:643	aerobic and anaerobic conditions	612:643	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	19	theme	compost-peat-sand	340:356	arg1	mixture					364:370	compost-peat-sand (CPS) mixture	340:370	compost-peat-sand (CPS) mixture	340:370	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	7	20	theme	atrazine-degrading	1365:1382	arg1	microorganisms					1384:1397	atrazine-degrading microorganisms	1365:1397	atrazine-degrading microorganisms	1365:1397	ADP amended slurries, although the sediment slurries were known to contain atrazine-degrading microorganisms.
29054658	9	21	dep	additives	1669:1677	arg1	additives					1669:1677	the additives NH4NO3 and NH4NO3	1665:1695	the additives NH4NO3 and NH4NO3	1665:1695	Atrazine degradation by indigenous microbes could not be stimulated by the surfactant methyl-β-cyclodextrin, or by the additives NH4NO3 and NH4NO3 with Na-citrate, although the nitrogen additives increased microbial growth.
29054658	9	21	dep	additives	1669:1677	arg1	NH4NO3					1690:1695	NH4NO3	1690:1695	NH4NO3	1690:1695	Atrazine degradation by indigenous microbes could not be stimulated by the surfactant methyl-β-cyclodextrin, or by the additives NH4NO3 and NH4NO3 with Na-citrate, although the nitrogen additives increased microbial growth.
29054658	9	21	dep	additives	1669:1677	arg1	NH4NO3					1679:1684	NH4NO3	1679:1684	NH4NO3	1679:1684	Atrazine degradation by indigenous microbes could not be stimulated by the surfactant methyl-β-cyclodextrin, or by the additives NH4NO3 and NH4NO3 with Na-citrate, although the nitrogen additives increased microbial growth.
29054658	8	22	theme	inorganic	1530:1538	arg1	colloids					1540:1547	organic and inorganic colloids	1518:1547	organic and inorganic colloids	1518:1547	The bioavailability of atrazine in the water phase seemed to be limited, which could be due to complex formation with organic and inorganic colloids.
29054658	2	23	theme	aerobic	612:618	arg1	conditions					634:643	aerobic and anaerobic conditions	612:643	aerobic and anaerobic conditions	612:643	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	0	24	dep	herbicide	83:91	arg1	dissipation					107:117	dissipation	107:117	dissipation	107:117	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	7	25	contain	contain	1357:1363	arg1	slurries					1334:1341	the sediment slurries	1321:1341	the sediment slurries	1321:1341	ADP amended slurries, although the sediment slurries were known to contain atrazine-degrading microorganisms.
29054658	7	25	contain	contain	1357:1363	arg2	microorganisms					1384:1397	atrazine-degrading microorganisms	1365:1397	atrazine-degrading microorganisms	1365:1397	ADP amended slurries, although the sediment slurries were known to contain atrazine-degrading microorganisms.
29054658	3	26	theme	zone	683:686	arg1	cores					697:701	The vadose zone sediment cores	672:701	The vadose zone sediment cores	672:701	The vadose zone sediment cores were drilled from a depth of 11.3-14.6m in an herbicide-contaminated groundwater area.
29054658	2	27	theme	surfactant	430:439	arg1	additions					463:471	the surfactant methyl-β-cyclodextrin additions	426:471	the surfactant methyl-β-cyclodextrin additions	426:471	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	5	28	theme	BAM	992:994	arg1	dissipation					996:1006	BAM dissipation	992:1006	BAM dissipation	992:1006	Dichlobenil dissipated under all conditions, while BAM dissipation was fairly slow and half-lives could not be calculated.
29054658	6	29	located	observed	1258:1265	arg2	degradation					1237:1247	pH. Microbial atrazine degradation	1214:1247	pH. Microbial atrazine degradation	1214:1247	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	6	29	located	observed	1258:1265	arg1	sp					1286:1287	the Pseudomonas sp	1270:1287	the Pseudomonas sp	1270:1287	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	2	30	theme	methyl-β-cyclodextrin	441:461	arg1	additions					463:471	the surfactant methyl-β-cyclodextrin additions	426:471	the surfactant methyl-β-cyclodextrin additions	426:471	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	6	31	theme	dissipation	1077:1087	arg1	rates					1089:1093	The chemical dissipation rates	1064:1093	The chemical dissipation rates	1064:1093	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	6	32	theme	Pseudomonas	1274:1284	arg1	sp					1286:1287	the Pseudomonas sp	1270:1287	the Pseudomonas sp	1270:1287	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	2	33	theme	sediment	588:595	arg1	slurries					597:604	sediment slurries	588:604	sediment slurries	588:604	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	34	theme	sterilized	651:660	arg1	controls					662:669	sterilized controls	651:669	sterilized controls	651:669	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	35	from	simazine	491:498	arg1	slurries					597:604	sediment slurries	588:604	sediment slurries	588:604	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	3	36	theme	groundwater	772:782	arg1	area					784:787	an herbicide-contaminated groundwater area	746:787	an herbicide-contaminated groundwater area	746:787	The vadose zone sediment cores were drilled from a depth of 11.3-14.6m in an herbicide-contaminated groundwater area.
29054658	6	37	theme	chemical	1068:1075	arg1	rates					1089:1093	The chemical dissipation rates	1064:1093	The chemical dissipation rates	1064:1093	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	0	38	theme	matter	21:26	arg1	Influence					0:8	Influence	0:8	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.	0:150	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	1	39	theme	cleaning	262:269	arg1	methods					271:277	no cleaning methods	259:277	no cleaning methods	259:277	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
29054658	6	40	theme	atrazine	1228:1235	arg1	degradation					1237:1247	pH. Microbial atrazine degradation	1214:1247	pH. Microbial atrazine degradation	1214:1247	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	8	41	theme	water	1439:1443	arg1	phase					1445:1449	the water phase	1435:1449	the water phase	1435:1449	The bioavailability of atrazine in the water phase seemed to be limited, which could be due to complex formation with organic and inorganic colloids.
29054658	2	42	theme	sodium	393:398	arg1	Na-citrate					409:418	Na-citrate	409:418	Na-citrate	409:418	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	42	theme	sodium	393:398	arg1	citrate					400:406	sodium citrate	393:406	sodium citrate (Na-citrate)	393:419	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	0	43	theme	organic	13:19	arg1	matter					21:26	organic matter	13:26	organic matter	13:26	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	6	44	theme	additive	1182:1189	arg1	compositions					1191:1202	additive compositions	1182:1202	additive compositions	1182:1202	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	7	45	theme	sediment	1325:1332	arg1	slurries					1334:1341	the sediment slurries	1321:1341	the sediment slurries	1321:1341	ADP amended slurries, although the sediment slurries were known to contain atrazine-degrading microorganisms.
29054658	6	46	theme	herbicides	1166:1175	arg1	structures					1133:1142	chemical structures	1124:1142	chemical structures	1124:1142	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	6	46	theme	herbicides	1166:1175	arg1	properties					1148:1157	properties	1148:1157	properties	1148:1157	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	0	47	theme	nutrients	29:37	arg1	Influence					0:8	Influence	0:8	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.	0:150	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	2	48	from	dissipations	572:583	arg1	slurries					597:604	sediment slurries	588:604	sediment slurries	588:604	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	0	49	theme	sediment	133:140	arg1	slurries					142:149	subsurface sediment slurries	122:149	subsurface sediment slurries	122:149	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	8	50	with	formation	1503:1511	arg1	colloids					1540:1547	organic and inorganic colloids	1518:1547	organic and inorganic colloids	1518:1547	The bioavailability of atrazine in the water phase seemed to be limited, which could be due to complex formation with organic and inorganic colloids.
29054658	9	51	theme	microbial	1756:1764	arg1	growth					1766:1771	microbial growth	1756:1771	microbial growth	1756:1771	Atrazine degradation by indigenous microbes could not be stimulated by the surfactant methyl-β-cyclodextrin, or by the additives NH4NO3 and NH4NO3 with Na-citrate, although the nitrogen additives increased microbial growth.
29054658	3	52	theme	herbicide-contaminated	749:770	arg1	area					784:787	an herbicide-contaminated groundwater area	746:787	an herbicide-contaminated groundwater area	746:787	The vadose zone sediment cores were drilled from a depth of 11.3-14.6m in an herbicide-contaminated groundwater area.
29054658	0	53	theme	subsurface	122:131	arg1	slurries					142:149	subsurface sediment slurries	122:149	subsurface sediment slurries	122:149	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	2	54	from	atrazine	481:488	arg1	slurries					597:604	sediment slurries	588:604	sediment slurries	588:604	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	8	55	theme	atrazine	1423:1430	arg1	limited					1464:1470	limited	1464:1470	limited	1464:1470	The bioavailability of atrazine in the water phase seemed to be limited, which could be due to complex formation with organic and inorganic colloids.
29054658	8	55	theme	atrazine	1423:1430	arg1	bioavailability					1404:1418	The bioavailability	1400:1418	The bioavailability of atrazine in the water phase	1400:1449	The bioavailability of atrazine in the water phase seemed to be limited, which could be due to complex formation with organic and inorganic colloids.
29054658	9	56	theme	surfactant	1625:1634	arg1	methyl-β-cyclodextrin					1636:1656	the surfactant methyl-β-cyclodextrin	1621:1656	the surfactant methyl-β-cyclodextrin	1621:1656	Atrazine degradation by indigenous microbes could not be stimulated by the surfactant methyl-β-cyclodextrin, or by the additives NH4NO3 and NH4NO3 with Na-citrate, although the nitrogen additives increased microbial growth.
29054658	1	57	theme	groundwater	289:299	arg1	environment					301:311	groundwater environment	289:311	groundwater environment	289:311	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
29054658	4	58	theme	chemical	882:889	arg1	dissipation					902:912	chemical hexazinone dissipation	882:912	chemical hexazinone dissipation	882:912	The peat and CPS enhanced chemical atrazine and simazine dissipation, and the peat enhanced chemical hexazinone dissipation, all oxygen-independently.
29054658	3	59	theme	11.3-14.6m	732:741	arg1	depth					723:727	a depth	721:727	a depth of 11.3-14.6m in an herbicide-contaminated groundwater area	721:787	The vadose zone sediment cores were drilled from a depth of 11.3-14.6m in an herbicide-contaminated groundwater area.
29054658	4	60	theme	chemical	816:823	arg1	atrazine					825:832	chemical atrazine	816:832	chemical atrazine	816:832	The peat and CPS enhanced chemical atrazine and simazine dissipation, and the peat enhanced chemical hexazinone dissipation, all oxygen-independently.
29054658	3	61	theme	vadose	676:681	arg1	cores					697:701	The vadose zone sediment cores	672:701	The vadose zone sediment cores	672:701	The vadose zone sediment cores were drilled from a depth of 11.3-14.6m in an herbicide-contaminated groundwater area.
29054658	6	62	theme	compositions	1191:1202	arg1	structures					1133:1142	chemical structures	1124:1142	chemical structures	1124:1142	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	6	62	theme	compositions	1191:1202	arg1	properties					1148:1157	properties	1148:1157	properties	1148:1157	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	2	63	theme	2,6-dichlorobenzamide	544:564	arg1	dissipations					572:583	the degradate 2,6-dichlorobenzamide (BAM) dissipations	530:583	the degradate 2,6-dichlorobenzamide (BAM) dissipations	530:583	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	0	64	from	degradate	97:105	arg1	slurries					142:149	subsurface sediment slurries	122:149	subsurface sediment slurries	122:149	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	3	65	from	depth	723:727	arg1	area					784:787	an herbicide-contaminated groundwater area	746:787	an herbicide-contaminated groundwater area	746:787	The vadose zone sediment cores were drilled from a depth of 11.3-14.6m in an herbicide-contaminated groundwater area.
29054658	2	66	with	peat	334:337	arg1	Na-citrate					409:418	Na-citrate	409:418	Na-citrate	409:418	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	66	with	peat	334:337	arg1	citrate					400:406	sodium citrate	393:406	sodium citrate (Na-citrate)	393:419	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	67	theme	degradate	534:542	arg1	dissipations					572:583	the degradate 2,6-dichlorobenzamide (BAM) dissipations	530:583	the degradate 2,6-dichlorobenzamide (BAM) dissipations	530:583	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	6	68	dep	structures	1133:1142	arg1	the					1120:1122	the	1120:1122	the	1120:1122	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	6	69	theme	Microbial	1218:1226	arg1	degradation					1237:1247	pH. Microbial atrazine degradation	1214:1247	pH. Microbial atrazine degradation	1214:1247	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	1	70	from	groundwater	197:207	arg1	threat					222:227	a global threat	213:227	a global threat for drinking water safety	213:253	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
29054658	1	70	from	groundwater	197:207	arg1	leaching					163:170	Pesticides leaching	152:170	Pesticides leaching from soil to surface and groundwater	152:207	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
29054658	1	71	theme	global	215:220	arg1	threat					222:227	a global threat	213:227	a global threat for drinking water safety	213:253	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
29054658	1	71	theme	global	215:220	arg1	leaching					163:170	Pesticides leaching	152:170	Pesticides leaching from soil to surface and groundwater	152:207	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
29054658	4	72	theme	hexazinone	891:900	arg1	dissipation					902:912	chemical hexazinone dissipation	882:912	chemical hexazinone dissipation	882:912	The peat and CPS enhanced chemical atrazine and simazine dissipation, and the peat enhanced chemical hexazinone dissipation, all oxygen-independently.
29054658	0	73	from	herbicide	83:91	arg1	slurries					142:149	subsurface sediment slurries	122:149	subsurface sediment slurries	122:149	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	6	74	theme	pH.	1214:1216	arg1	degradation					1237:1247	pH. Microbial atrazine degradation	1214:1247	pH. Microbial atrazine degradation	1214:1247	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	6	75	theme	chemical	1124:1131	arg1	structures					1133:1142	chemical structures	1124:1142	chemical structures	1124:1142	The chemical dissipation rates could be associated with the chemical structures and properties of the herbicides, and additive compositions, not with pH. Microbial atrazine degradation was only observed in the Pseudomonas sp.
29054658	2	76	theme	CPS	359:361	arg1	mixture					364:370	compost-peat-sand (CPS) mixture	340:370	compost-peat-sand (CPS) mixture	340:370	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	0	77	theme	cyclodextrin	44:55	arg1	Influence					0:8	Influence	0:8	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.	0:150	Influence of organic matter, nutrients, and cyclodextrin on microbial and chemical herbicide and degradate dissipation in subsurface sediment slurries.
29054658	2	78	with	mixture	364:370	arg1	Na-citrate					409:418	Na-citrate	409:418	Na-citrate	409:418	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	2	78	with	mixture	364:370	arg1	citrate					400:406	sodium citrate	393:406	sodium citrate (Na-citrate)	393:419	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	4	79	dep	atrazine	825:832	arg1	dissipation					847:857	dissipation	847:857	dissipation	847:857	The peat and CPS enhanced chemical atrazine and simazine dissipation, and the peat enhanced chemical hexazinone dissipation, all oxygen-independently.
29054658	2	80	from	dichlobenil	513:523	arg1	slurries					597:604	sediment slurries	588:604	sediment slurries	588:604	We examined whether peat, compost-peat-sand (CPS) mixture, NH4NO3, NH4NO3 with sodium citrate (Na-citrate), and the surfactant methyl-β-cyclodextrin additions enhance atrazine, simazine, hexazinone, dichlobenil, and the degradate 2,6-dichlorobenzamide (BAM) dissipations in sediment slurries under aerobic and anaerobic conditions, with sterilized controls.
29054658	9	81	theme	nitrogen	1727:1734	arg1	additives					1736:1744	the nitrogen additives	1723:1744	the nitrogen additives	1723:1744	Atrazine degradation by indigenous microbes could not be stimulated by the surfactant methyl-β-cyclodextrin, or by the additives NH4NO3 and NH4NO3 with Na-citrate, although the nitrogen additives increased microbial growth.
29054658	1	82	theme	drinking	233:240	arg1	water					242:246	drinking water	233:246	drinking water safety	233:253	Pesticides leaching from soil to surface and groundwater are a global threat for drinking water safety, as no cleaning methods occur for groundwater environment.
27620464	3	0	theme	regular	350:356	arg1	vesicles					358:365	regular vesicles	350:365	regular vesicles (transfersomes and penetration enhancer vesicles)	350:415	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	4	1	from	potential	675:683	arg1	bilayer					714:720	the vesicular bilayer	700:720	the vesicular bilayer	700:720	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	3	2	theme	verapamil	553:561	arg1	VRP					577:579	a model antihypertensive drug; verapamil hydrochloride VRP	522:579	a model antihypertensive drug; verapamil hydrochloride VRP	522:579	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	4	3	dep	negative	868:875	arg1	positive					880:887	positive	880:887	positive	880:887	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	4	3	dep	negative	868:875	arg1	to					877:878	to	877:878	to	877:878	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	0	4	theme	verapamil	100:108	arg1	bioavailability					81:95	bioavailability	81:95	bioavailability of verapamil	81:108	Composite chitosan-transfersomal vesicles for improved transnasal permeation and bioavailability of verapamil.
27620464	0	4	theme	verapamil	100:108	arg1	permeation					66:75	improved transnasal permeation	46:75	improved transnasal permeation	46:75	Composite chitosan-transfersomal vesicles for improved transnasal permeation and bioavailability of verapamil.
27620464	3	5	theme	hydrochloride	563:575	arg1	VRP					577:579	a model antihypertensive drug; verapamil hydrochloride VRP	522:579	a model antihypertensive drug; verapamil hydrochloride VRP	522:579	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	3	6	theme	enhancer	398:405	arg1	vesicles					407:414	penetration enhancer vesicles	386:414	penetration enhancer vesicles	386:414	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	6	7	from	formulation	1127:1137	arg1	release					1094:1100	the in vitro release	1081:1100	the in vitro release of VRP from the selected formulation	1081:1137	Chitosan slowed the in vitro release of VRP from the selected formulation but managed to achieve high penetrability across sheep nasal mucosa as displayed by confocal laser microscopy.
27620464	5	8	theme	transfersomal	1006:1018	arg1	vesicles					1020:1027	transfersomal vesicles	1006:1027	transfersomal vesicles prepared using sodium deoxycholate	1006:1062	The entrapment efficiency of VRP in the vesicles ranged from 24 to 64%, with best physical stability displayed with transfersomal vesicles prepared using sodium deoxycholate.
27620464	8	9	with	drugs	1556:1560	arg1	bioavailability					1576:1590	low oral bioavailability	1567:1590	low oral bioavailability	1567:1590	Findings of this manuscript highly recommend chitosan as a promising functional additive in vesicular formulations to improve the intranasal delivery of drugs with low oral bioavailability.
27620464	1	10	theme	drug	177:180	arg1	delivery					182:189	drug delivery	177:189	drug delivery	177:189	The creation of composite systems has become an emerging field in drug delivery.
27620464	5	11	theme	physical	972:979	arg1	stability					981:989	best physical stability	967:989	best physical stability displayed with transfersomal vesicles prepared using sodium deoxycholate	967:1062	The entrapment efficiency of VRP in the vesicles ranged from 24 to 64%, with best physical stability displayed with transfersomal vesicles prepared using sodium deoxycholate.
27620464	3	12	dep	vesicles	431:438	arg1	vesicles					500:507	penetration enhancer vesicles	479:507	penetration enhancer vesicles	479:507	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	3	12	dep	vesicles	431:438	arg1	transfersomes					461:473	chitosan containing transfersomes	441:473	chitosan containing transfersomes	441:473	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	5	13	theme	best	967:970	arg1	stability					981:989	best physical stability	967:989	best physical stability displayed with transfersomal vesicles prepared using sodium deoxycholate	967:1062	The entrapment efficiency of VRP in the vesicles ranged from 24 to 64%, with best physical stability displayed with transfersomal vesicles prepared using sodium deoxycholate.
27620464	4	14	theme	shift	828:832	arg1	values					856:861	an increased viscosity of composite vesicles and a shift in the zeta potential values	777:861	an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive	777:887	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	4	15	theme	viscosity	790:798	arg1	values					856:861	an increased viscosity of composite vesicles and a shift in the zeta potential values	777:861	an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive	777:887	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	1	16	theme	systems	137:143	arg1	creation					115:122	The creation	111:122	The creation of composite systems	111:143	The creation of composite systems has become an emerging field in drug delivery.
27620464	1	17	from	field	168:172	arg1	delivery					182:189	drug delivery	177:189	drug delivery	177:189	The creation of composite systems has become an emerging field in drug delivery.
27620464	4	18	theme	zeta	841:844	arg1	potential					846:854	the zeta potential	837:854	the zeta potential	837:854	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	6	19	theme	selected	1118:1125	arg1	formulation					1127:1137	the selected formulation	1114:1137	the selected formulation	1114:1137	Chitosan slowed the in vitro release of VRP from the selected formulation but managed to achieve high penetrability across sheep nasal mucosa as displayed by confocal laser microscopy.
27620464	3	20	theme	enhancer	491:498	arg1	vesicles					500:507	penetration enhancer vesicles	479:507	penetration enhancer vesicles	479:507	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	3	21	theme	penetration	386:396	arg1	vesicles					407:414	penetration enhancer vesicles	386:414	penetration enhancer vesicles	386:414	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	3	22	theme	containing	450:459	arg1	transfersomes					461:473	chitosan containing transfersomes	441:473	chitosan containing transfersomes	441:473	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	7	23	theme	composite	1263:1271	arg1	formulation					1287:1297	The chitosan composite transfersomal formulation	1250:1297	The chitosan composite transfersomal formulation	1250:1297	The chitosan composite transfersomal formulation exhibited absolute bioavailability of 81.83% compared to the oral solution which displayed only 13.04%.
27620464	6	24	theme	confocal	1223:1230	arg1	microscopy					1238:1247	confocal laser microscopy	1223:1247	confocal laser microscopy	1223:1247	Chitosan slowed the in vitro release of VRP from the selected formulation but managed to achieve high penetrability across sheep nasal mucosa as displayed by confocal laser microscopy.
27620464	8	25	from	additive	1483:1490	arg1	formulations					1505:1516	vesicular formulations	1495:1516	vesicular formulations	1495:1516	Findings of this manuscript highly recommend chitosan as a promising functional additive in vesicular formulations to improve the intranasal delivery of drugs with low oral bioavailability.
27620464	0	26	theme	chitosan-transfersomal	10:31	arg1	vesicles					33:40	Composite chitosan-transfersomal vesicles	0:40	Composite chitosan-transfersomal vesicles for improved transnasal permeation and bioavailability of verapamil.	0:109	Composite chitosan-transfersomal vesicles for improved transnasal permeation and bioavailability of verapamil.
27620464	6	27	dep	in	1085:1086	arg1	vitro					1088:1092	vitro	1088:1092	vitro	1088:1092	Chitosan slowed the in vitro release of VRP from the selected formulation but managed to achieve high penetrability across sheep nasal mucosa as displayed by confocal laser microscopy.
27620464	4	28	theme	vesicles	813:820	arg1	viscosity					790:798	an increased viscosity	777:798	an increased viscosity of composite vesicles	777:820	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	4	28	theme	vesicles	813:820	arg1	shift					828:832	a shift	826:832	a shift in the zeta potential	826:854	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	4	29	theme	transmission	738:749	arg1	microscopy					760:769	transmission electron microscopy	738:769	transmission electron microscopy	738:769	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	0	30	theme	Composite	0:8	arg1	vesicles					33:40	Composite chitosan-transfersomal vesicles	0:40	Composite chitosan-transfersomal vesicles for improved transnasal permeation and bioavailability of verapamil.	0:109	Composite chitosan-transfersomal vesicles for improved transnasal permeation and bioavailability of verapamil.
27620464	8	31	theme	oral	1571:1574	arg1	bioavailability					1576:1590	low oral bioavailability	1567:1590	low oral bioavailability	1567:1590	Findings of this manuscript highly recommend chitosan as a promising functional additive in vesicular formulations to improve the intranasal delivery of drugs with low oral bioavailability.
27620464	4	32	theme	composite	803:811	arg1	vesicles					813:820	composite vesicles	803:820	composite vesicles	803:820	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	3	33	theme	penetration	479:489	arg1	vesicles					500:507	penetration enhancer vesicles	479:507	penetration enhancer vesicles	479:507	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	4	34	theme	particle	618:625	arg1	size					627:630	larger particle size	611:630	larger particle size	611:630	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	4	35	from	shift	828:832	arg1	potential					846:854	the zeta potential	837:854	the zeta potential	837:854	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	5	36	theme	entrapment	894:903	arg1	efficiency					905:914	The entrapment efficiency	890:914	The entrapment efficiency of VRP in the vesicles	890:937	The entrapment efficiency of VRP in the vesicles ranged from 24 to 64%, with best physical stability displayed with transfersomal vesicles prepared using sodium deoxycholate.
27620464	5	37	dep	64	957:958	arg1	to					954:955	to	954:955	to	954:955	The entrapment efficiency of VRP in the vesicles ranged from 24 to 64%, with best physical stability displayed with transfersomal vesicles prepared using sodium deoxycholate.
27620464	4	38	theme	regular	637:643	arg1	vesicles					645:652	regular vesicles	637:652	regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy	637:769	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	3	39	theme	comparative	310:320	arg1	study					322:326	a comparative study	308:326	a comparative study	308:326	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	1	40	theme	composite	127:135	arg1	systems					137:143	composite systems	127:143	composite systems	127:143	The creation of composite systems has become an emerging field in drug delivery.
27620464	6	41	theme	VRP	1105:1107	arg1	release					1094:1100	the in vitro release	1081:1100	the in vitro release of VRP from the selected formulation	1081:1137	Chitosan slowed the in vitro release of VRP from the selected formulation but managed to achieve high penetrability across sheep nasal mucosa as displayed by confocal laser microscopy.
27620464	0	42	theme	transnasal	55:64	arg1	permeation					66:75	improved transnasal permeation	46:75	improved transnasal permeation	46:75	Composite chitosan-transfersomal vesicles for improved transnasal permeation and bioavailability of verapamil.
27620464	7	43	theme	%	1342:1342	arg1	bioavailability					1318:1332	absolute bioavailability	1309:1332	absolute bioavailability of 81.83%	1309:1342	The chitosan composite transfersomal formulation exhibited absolute bioavailability of 81.83% compared to the oral solution which displayed only 13.04%.
27620464	2	44	theme	intranasal	267:276	arg1	delivery					278:285	intranasal delivery	267:285	intranasal delivery	267:285	Chitosan has demonstrated several pharmaceutical advantages, especially in intranasal delivery.
27620464	8	45	from	formulations	1505:1516	arg1	additive					1483:1490	additive	1483:1490	additive	1483:1490	Findings of this manuscript highly recommend chitosan as a promising functional additive in vesicular formulations to improve the intranasal delivery of drugs with low oral bioavailability.
27620464	8	45	from	formulations	1505:1516	arg1	chitosan					1448:1455	chitosan	1448:1455	chitosan	1448:1455	Findings of this manuscript highly recommend chitosan as a promising functional additive in vesicular formulations to improve the intranasal delivery of drugs with low oral bioavailability.
27620464	0	46	theme	improved	46:53	arg1	permeation					66:75	improved transnasal permeation	46:75	improved transnasal permeation	46:75	Composite chitosan-transfersomal vesicles for improved transnasal permeation and bioavailability of verapamil.
27620464	8	47	theme	drugs	1556:1560	arg1	delivery					1544:1551	the intranasal delivery	1529:1551	the intranasal delivery of drugs with low oral bioavailability	1529:1590	Findings of this manuscript highly recommend chitosan as a promising functional additive in vesicular formulations to improve the intranasal delivery of drugs with low oral bioavailability.
27620464	3	48	dep	vesicles	358:365	arg1	transfersomes					368:380	transfersomes	368:380	transfersomes	368:380	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	3	48	dep	vesicles	358:365	arg1	vesicles					407:414	penetration enhancer vesicles	386:414	penetration enhancer vesicles	386:414	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	4	49	theme	vesicular	704:712	arg1	bilayer					714:720	the vesicular bilayer	700:720	the vesicular bilayer	700:720	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	4	50	theme	Composite	582:590	arg1	vesicles					592:599	Composite vesicles	582:599	Composite vesicles	582:599	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	8	51	theme	vesicular	1495:1503	arg1	formulations					1505:1516	vesicular formulations	1495:1516	vesicular formulations	1495:1516	Findings of this manuscript highly recommend chitosan as a promising functional additive in vesicular formulations to improve the intranasal delivery of drugs with low oral bioavailability.
27620464	6	52	theme	high	1162:1165	arg1	penetrability					1167:1179	high penetrability	1162:1179	high penetrability across sheep nasal mucosa	1162:1205	Chitosan slowed the in vitro release of VRP from the selected formulation but managed to achieve high penetrability across sheep nasal mucosa as displayed by confocal laser microscopy.
27620464	3	53	theme	drug	547:550	arg1	VRP					577:579	a model antihypertensive drug; verapamil hydrochloride VRP	522:579	a model antihypertensive drug; verapamil hydrochloride VRP	522:579	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	3	54	theme	chitosan	441:448	arg1	transfersomes					461:473	chitosan containing transfersomes	441:473	chitosan containing transfersomes	441:473	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	4	55	theme	larger	611:616	arg1	size					627:630	larger particle size	611:630	larger particle size	611:630	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	7	56	theme	chitosan	1254:1261	arg1	formulation					1287:1297	The chitosan composite transfersomal formulation	1250:1297	The chitosan composite transfersomal formulation	1250:1297	The chitosan composite transfersomal formulation exhibited absolute bioavailability of 81.83% compared to the oral solution which displayed only 13.04%.
27620464	8	57	theme	low	1567:1569	arg1	bioavailability					1576:1590	low oral bioavailability	1567:1590	low oral bioavailability	1567:1590	Findings of this manuscript highly recommend chitosan as a promising functional additive in vesicular formulations to improve the intranasal delivery of drugs with low oral bioavailability.
27620464	7	58	theme	oral	1360:1363	arg1	solution					1365:1372	the oral solution	1356:1372	the oral solution which displayed only 13.04%	1356:1400	The chitosan composite transfersomal formulation exhibited absolute bioavailability of 81.83% compared to the oral solution which displayed only 13.04%.
27620464	6	59	theme	sheep	1188:1192	arg1	mucosa					1200:1205	sheep nasal mucosa	1188:1205	sheep nasal mucosa	1188:1205	Chitosan slowed the in vitro release of VRP from the selected formulation but managed to achieve high penetrability across sheep nasal mucosa as displayed by confocal laser microscopy.
27620464	8	60	theme	intranasal	1533:1542	arg1	delivery					1544:1551	the intranasal delivery	1529:1551	the intranasal delivery of drugs with low oral bioavailability	1529:1590	Findings of this manuscript highly recommend chitosan as a promising functional additive in vesicular formulations to improve the intranasal delivery of drugs with low oral bioavailability.
27620464	6	61	theme	in	1085:1086	arg1	release					1094:1100	the in vitro release	1081:1100	the in vitro release of VRP from the selected formulation	1081:1137	Chitosan slowed the in vitro release of VRP from the selected formulation but managed to achieve high penetrability across sheep nasal mucosa as displayed by confocal laser microscopy.
27620464	4	62	theme	increased	780:788	arg1	viscosity					790:798	an increased viscosity	777:798	an increased viscosity of composite vesicles	777:820	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	2	63	theme	pharmaceutical	226:239	arg1	advantages					241:250	several pharmaceutical advantages	218:250	several pharmaceutical advantages	218:250	Chitosan has demonstrated several pharmaceutical advantages, especially in intranasal delivery.
27620464	5	64	theme	sodium	1044:1049	arg1	deoxycholate					1051:1062	sodium deoxycholate	1044:1062	sodium deoxycholate	1044:1062	The entrapment efficiency of VRP in the vesicles ranged from 24 to 64%, with best physical stability displayed with transfersomal vesicles prepared using sodium deoxycholate.
27620464	4	65	theme	electron	751:758	arg1	microscopy					760:769	transmission electron microscopy	738:769	transmission electron microscopy	738:769	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	3	66	theme	model	524:528	arg1	VRP					577:579	a model antihypertensive drug; verapamil hydrochloride VRP	522:579	a model antihypertensive drug; verapamil hydrochloride VRP	522:579	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	4	67	from	viscosity	790:798	arg1	potential					846:854	the zeta potential	837:854	the zeta potential	837:854	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	5	68	from	efficiency	905:914	arg1	vesicles					930:937	the vesicles	926:937	the vesicles	926:937	The entrapment efficiency of VRP in the vesicles ranged from 24 to 64%, with best physical stability displayed with transfersomal vesicles prepared using sodium deoxycholate.
27620464	2	69	theme	several	218:224	arg1	advantages					241:250	several pharmaceutical advantages	218:250	several pharmaceutical advantages	218:250	Chitosan has demonstrated several pharmaceutical advantages, especially in intranasal delivery.
27620464	8	70	theme	manuscript	1420:1429	arg1	Findings					1403:1410	Findings	1403:1410	Findings of this manuscript	1403:1429	Findings of this manuscript highly recommend chitosan as a promising functional additive in vesicular formulations to improve the intranasal delivery of drugs with low oral bioavailability.
27620464	3	71	theme	antihypertensive	530:545	arg1	VRP					577:579	a model antihypertensive drug; verapamil hydrochloride VRP	522:579	a model antihypertensive drug; verapamil hydrochloride VRP	522:579	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	6	72	theme	nasal	1194:1198	arg1	mucosa					1200:1205	sheep nasal mucosa	1188:1205	sheep nasal mucosa	1188:1205	Chitosan slowed the in vitro release of VRP from the selected formulation but managed to achieve high penetrability across sheep nasal mucosa as displayed by confocal laser microscopy.
27620464	4	73	theme	coating	667:673	arg1	potential					675:683	the coating potential	663:683	the coating potential of chitosan on the vesicular bilayer	663:720	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	7	74	theme	absolute	1309:1316	arg1	bioavailability					1318:1332	absolute bioavailability	1309:1332	absolute bioavailability of 81.83%	1309:1342	The chitosan composite transfersomal formulation exhibited absolute bioavailability of 81.83% compared to the oral solution which displayed only 13.04%.
27620464	5	75	theme	VRP	919:921	arg1	efficiency					905:914	The entrapment efficiency	890:914	The entrapment efficiency of VRP in the vesicles	890:937	The entrapment efficiency of VRP in the vesicles ranged from 24 to 64%, with best physical stability displayed with transfersomal vesicles prepared using sodium deoxycholate.
27620464	1	76	theme	emerging	159:166	arg1	field					168:172	an emerging field	156:172	an emerging field in drug delivery	156:189	The creation of composite systems has become an emerging field in drug delivery.
27620464	6	77	theme	laser	1232:1236	arg1	microscopy					1238:1247	confocal laser microscopy	1223:1247	confocal laser microscopy	1223:1247	Chitosan slowed the in vitro release of VRP from the selected formulation but managed to achieve high penetrability across sheep nasal mucosa as displayed by confocal laser microscopy.
27620464	4	78	theme	chitosan	688:695	arg1	potential					675:683	the coating potential	663:683	the coating potential of chitosan on the vesicular bilayer	663:720	Composite vesicles displayed larger particle size than regular vesicles owing to the coating potential of chitosan on the vesicular bilayer as displayed by transmission electron microscopy, with an increased viscosity of composite vesicles and a shift in the zeta potential values from negative to positive.
27620464	3	79	theme	composite	421:429	arg1	vesicles					431:438	composite vesicles	421:438	composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles)	421:508	In this manuscript, a comparative study was conducted between regular vesicles (transfersomes and penetration enhancer vesicles) and composite vesicles (chitosan containing transfersomes and penetration enhancer vesicles) loaded with a model antihypertensive drug; verapamil hydrochloride VRP.
27620464	7	80	theme	transfersomal	1273:1285	arg1	formulation					1287:1297	The chitosan composite transfersomal formulation	1250:1297	The chitosan composite transfersomal formulation	1250:1297	The chitosan composite transfersomal formulation exhibited absolute bioavailability of 81.83% compared to the oral solution which displayed only 13.04%.
29100158	9	0	theme	efficient	1411:1419	arg1	stabilization					1430:1442	efficient physical stabilization	1411:1442	efficient physical stabilization	1411:1442	Retaining the native interactions of GGM and phenolic residues is suggested for efficient physical stabilization and extended protection against lipid oxidation.
29100158	10	1	theme	interfacial	1601:1611	arg1	stabilization					1613:1625	interfacial stabilization	1601:1625	interfacial stabilization	1601:1625	The results can be widely applied as guidelines in tailoring natural or synthetic amphiphilic compounds for interfacial stabilization.
29100158	7	2	theme	superior	1147:1154	arg1	stabilization					1156:1168	superior stabilization	1147:1168	superior stabilization of emulsions	1147:1181	FINDINGS Naturally associated lignin residues in GGM act as vehicles for anchoring these hemicelluloses into the oil droplet interface and further enable superior stabilization of emulsions.
29100158	7	2	theme	superior	1147:1154	arg1	FINDINGS					993:1000	FINDINGS	993:1000	FINDINGS Naturally associated lignin residues in GGM	993:1044	FINDINGS Naturally associated lignin residues in GGM act as vehicles for anchoring these hemicelluloses into the oil droplet interface and further enable superior stabilization of emulsions.
29100158	7	2	theme	superior	1147:1154	arg1	vehicles					1053:1060	vehicles	1053:1060	vehicles for anchoring these hemicelluloses into the oil droplet interface	1053:1126	FINDINGS Naturally associated lignin residues in GGM act as vehicles for anchoring these hemicelluloses into the oil droplet interface and further enable superior stabilization of emulsions.
29100158	1	3	theme	dispersed	177:185	arg1	emulsions					204:212	emulsions	204:212	emulsions	204:212	HYPOTHESIS Amphiphilic character of surfactants drives them at the interface of dispersed systems, such as emulsions.
29100158	1	3	theme	dispersed	177:185	arg1	systems					187:193	dispersed systems	177:193	dispersed systems	177:193	HYPOTHESIS Amphiphilic character of surfactants drives them at the interface of dispersed systems, such as emulsions.
29100158	0	4	from	residues	9:16	arg1	galactoglucomannans					28:46	spruce galactoglucomannans	21:46	spruce galactoglucomannans	21:46	Phenolic residues in spruce galactoglucomannans improve stabilization of oil-in-water emulsions.
29100158	6	5	theme	physical	925:932	arg1	stability					934:942	the physical stability	921:942	the physical stability	921:942	Meanwhile, the physical stability and lipid oxidation in emulsions were monitored.
29100158	8	6	theme	interfacial	1293:1303	arg1	functionalities					1265:1279	their functionalities	1259:1279	their functionalities	1259:1279	By adjusting the isolation method of GGM regarding their phenolic profile, their functionalities, especially interfacial behavior, can be altered.
29100158	8	6	theme	interfacial	1293:1303	arg1	behavior					1305:1312	interfacial behavior	1293:1312	especially interfacial behavior	1282:1312	By adjusting the isolation method of GGM regarding their phenolic profile, their functionalities, especially interfacial behavior, can be altered.
29100158	1	7	theme	systems	187:193	arg1	interface					164:172	the interface	160:172	the interface of dispersed systems, such as emulsions	160:212	HYPOTHESIS Amphiphilic character of surfactants drives them at the interface of dispersed systems, such as emulsions.
29100158	10	8	theme	amphiphilic	1575:1585	arg1	compounds					1587:1595	natural or synthetic amphiphilic compounds	1554:1595	natural or synthetic amphiphilic compounds for interfacial stabilization	1554:1625	The results can be widely applied as guidelines in tailoring natural or synthetic amphiphilic compounds for interfacial stabilization.
29100158	5	9	theme	accelerated	884:894	arg1	test					904:907	an accelerated storage test	881:907	an accelerated storage test	881:907	Distribution and changes occurring in the phenolic residues at the droplet interface and in the continuous phase were studied during an accelerated storage test.
29100158	7	10	from	residues	1030:1037	arg1	GGM					1042:1044	GGM	1042:1044	GGM	1042:1044	FINDINGS Naturally associated lignin residues in GGM act as vehicles for anchoring these hemicelluloses into the oil droplet interface and further enable superior stabilization of emulsions.
29100158	5	11	from	residues	799:806	arg1	phase					855:859	the continuous phase	840:859	the continuous phase	840:859	Distribution and changes occurring in the phenolic residues at the droplet interface and in the continuous phase were studied during an accelerated storage test.
29100158	9	12	theme	lipid	1476:1480	arg1	oxidation					1482:1490	lipid oxidation	1476:1490	lipid oxidation	1476:1490	Retaining the native interactions of GGM and phenolic residues is suggested for efficient physical stabilization and extended protection against lipid oxidation.
29100158	0	13	theme	Phenolic	0:7	arg1	residues					9:16	Phenolic residues	0:16	Phenolic residues in spruce galactoglucomannans	0:46	Phenolic residues in spruce galactoglucomannans improve stabilization of oil-in-water emulsions.
29100158	9	14	theme	extended	1448:1455	arg1	protection					1457:1466	extended protection	1448:1466	extended protection against lipid oxidation	1448:1490	Retaining the native interactions of GGM and phenolic residues is suggested for efficient physical stabilization and extended protection against lipid oxidation.
29100158	2	15	theme	natural	309:315	arg1	amphiphilicity					317:330	natural amphiphilicity	309:330	natural amphiphilicity	309:330	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	6	16	from	stability	934:942	arg1	emulsions					967:975	emulsions	967:975	emulsions	967:975	Meanwhile, the physical stability and lipid oxidation in emulsions were monitored.
29100158	10	17	theme	synthetic	1565:1573	arg1	compounds					1587:1595	natural or synthetic amphiphilic compounds	1554:1595	natural or synthetic amphiphilic compounds for interfacial stabilization	1554:1625	The results can be widely applied as guidelines in tailoring natural or synthetic amphiphilic compounds for interfacial stabilization.
29100158	1	18	theme	HYPOTHESIS	97:106	arg1	character					120:128	HYPOTHESIS Amphiphilic character	97:128	HYPOTHESIS Amphiphilic character of surfactants	97:143	HYPOTHESIS Amphiphilic character of surfactants drives them at the interface of dispersed systems, such as emulsions.
29100158	4	19	dep	EXPERIMENTS	575:585	arg1	investigated					590:601	investigated	590:601	investigated the role of phenolic residues in spruce hemicellulose extracts in the stabilization of oil-in-water emulsions by physical and chemical approach	590:745	EXPERIMENTS We investigated the role of phenolic residues in spruce hemicellulose extracts in the stabilization of oil-in-water emulsions by physical and chemical approach.
29100158	6	20	from	oxidation	954:962	arg1	emulsions					967:975	emulsions	967:975	emulsions	967:975	Meanwhile, the physical stability and lipid oxidation in emulsions were monitored.
29100158	5	21	theme	continuous	844:853	arg1	phase					855:859	the continuous phase	840:859	the continuous phase	840:859	Distribution and changes occurring in the phenolic residues at the droplet interface and in the continuous phase were studied during an accelerated storage test.
29100158	1	22	theme	Amphiphilic	108:118	arg1	character					120:128	HYPOTHESIS Amphiphilic character	97:128	HYPOTHESIS Amphiphilic character of surfactants	97:143	HYPOTHESIS Amphiphilic character of surfactants drives them at the interface of dispersed systems, such as emulsions.
29100158	3	23	theme	Lignin-derived	428:441	arg1	residues					452:459	Lignin-derived phenolic residues	428:459	Lignin-derived phenolic residues associated with hemicelluloses	428:490	Lignin-derived phenolic residues associated with hemicelluloses are hypothesized to contribute to emulsions' interfacial properties and stability.
29100158	2	24	with	assemblies	256:265	arg1	amphiphilicity					317:330	natural amphiphilicity	309:330	natural amphiphilicity	309:330	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	10	25	theme	natural	1554:1560	arg1	compounds					1587:1595	natural or synthetic amphiphilic compounds	1554:1595	natural or synthetic amphiphilic compounds for interfacial stabilization	1554:1625	The results can be widely applied as guidelines in tailoring natural or synthetic amphiphilic compounds for interfacial stabilization.
29100158	2	26	theme	isolation	410:418	arg1	method					420:425	the isolation method	406:425	the isolation method	406:425	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	0	27	theme	spruce	21:26	arg1	galactoglucomannans					28:46	spruce galactoglucomannans	21:46	spruce galactoglucomannans	21:46	Phenolic residues in spruce galactoglucomannans improve stabilization of oil-in-water emulsions.
29100158	4	28	theme	residues	624:631	arg1	role					607:610	the role	603:610	the role of phenolic residues in spruce hemicellulose extracts	603:664	EXPERIMENTS We investigated the role of phenolic residues in spruce hemicellulose extracts in the stabilization of oil-in-water emulsions by physical and chemical approach.
29100158	8	29	theme	isolation	1201:1209	arg1	method					1211:1216	the isolation method	1197:1216	the isolation method of GGM regarding their phenolic profile	1197:1256	By adjusting the isolation method of GGM regarding their phenolic profile, their functionalities, especially interfacial behavior, can be altered.
29100158	9	30	theme	native	1345:1350	arg1	interactions					1352:1363	the native interactions	1341:1363	the native interactions of GGM and phenolic residues	1341:1392	Retaining the native interactions of GGM and phenolic residues is suggested for efficient physical stabilization and extended protection against lipid oxidation.
29100158	4	31	from	role	607:610	arg1	extracts					657:664	spruce hemicellulose extracts	636:664	spruce hemicellulose extracts	636:664	EXPERIMENTS We investigated the role of phenolic residues in spruce hemicellulose extracts in the stabilization of oil-in-water emulsions by physical and chemical approach.
29100158	2	32	dep	assemblies	256:265	arg1	LCC					299:301	LCC	299:301	LCC	299:301	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	2	32	dep	assemblies	256:265	arg1	complexes					288:296	lignin-carbohydrate complexes	268:296	lignin-carbohydrate complexes	268:296	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	4	33	theme	hemicellulose	643:655	arg1	extracts					657:664	spruce hemicellulose extracts	636:664	spruce hemicellulose extracts	636:664	EXPERIMENTS We investigated the role of phenolic residues in spruce hemicellulose extracts in the stabilization of oil-in-water emulsions by physical and chemical approach.
29100158	1	34	theme	surfactants	133:143	arg1	character					120:128	HYPOTHESIS Amphiphilic character	97:128	HYPOTHESIS Amphiphilic character of surfactants	97:143	HYPOTHESIS Amphiphilic character of surfactants drives them at the interface of dispersed systems, such as emulsions.
29100158	4	35	theme	physical	716:723	arg1	approach					738:745	physical and chemical approach	716:745	physical and chemical approach	716:745	EXPERIMENTS We investigated the role of phenolic residues in spruce hemicellulose extracts in the stabilization of oil-in-water emulsions by physical and chemical approach.
29100158	4	36	theme	spruce	636:641	arg1	extracts					657:664	spruce hemicellulose extracts	636:664	spruce hemicellulose extracts	636:664	EXPERIMENTS We investigated the role of phenolic residues in spruce hemicellulose extracts in the stabilization of oil-in-water emulsions by physical and chemical approach.
29100158	7	37	theme	emulsions	1173:1181	arg1	stabilization					1156:1168	superior stabilization	1147:1168	superior stabilization of emulsions	1147:1181	FINDINGS Naturally associated lignin residues in GGM act as vehicles for anchoring these hemicelluloses into the oil droplet interface and further enable superior stabilization of emulsions.
29100158	7	37	theme	emulsions	1173:1181	arg1	FINDINGS					993:1000	FINDINGS	993:1000	FINDINGS Naturally associated lignin residues in GGM	993:1044	FINDINGS Naturally associated lignin residues in GGM act as vehicles for anchoring these hemicelluloses into the oil droplet interface and further enable superior stabilization of emulsions.
29100158	7	37	theme	emulsions	1173:1181	arg1	vehicles					1053:1060	vehicles	1053:1060	vehicles for anchoring these hemicelluloses into the oil droplet interface	1053:1126	FINDINGS Naturally associated lignin residues in GGM act as vehicles for anchoring these hemicelluloses into the oil droplet interface and further enable superior stabilization of emulsions.
29100158	2	38	theme	lignin-carbohydrate	268:286	arg1	LCC					299:301	LCC	299:301	LCC	299:301	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	2	38	theme	lignin-carbohydrate	268:286	arg1	complexes					288:296	lignin-carbohydrate complexes	268:296	lignin-carbohydrate complexes	268:296	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	6	39	from	Meanwhile	910:918	arg1	emulsions					967:975	emulsions	967:975	emulsions	967:975	Meanwhile, the physical stability and lipid oxidation in emulsions were monitored.
29100158	5	40	theme	droplet	815:821	arg1	interface					823:831	the droplet interface	811:831	the droplet interface	811:831	Distribution and changes occurring in the phenolic residues at the droplet interface and in the continuous phase were studied during an accelerated storage test.
29100158	5	41	from	interface	823:831	arg1	residues					799:806	the phenolic residues	786:806	the phenolic residues at the droplet interface and in the continuous phase	786:859	Distribution and changes occurring in the phenolic residues at the droplet interface and in the continuous phase were studied during an accelerated storage test.
29100158	4	42	theme	chemical	729:736	arg1	approach					738:745	physical and chemical approach	716:745	physical and chemical approach	716:745	EXPERIMENTS We investigated the role of phenolic residues in spruce hemicellulose extracts in the stabilization of oil-in-water emulsions by physical and chemical approach.
29100158	3	43	theme	phenolic	443:450	arg1	residues					452:459	Lignin-derived phenolic residues	428:459	Lignin-derived phenolic residues associated with hemicelluloses	428:490	Lignin-derived phenolic residues associated with hemicelluloses are hypothesized to contribute to emulsions' interfacial properties and stability.
29100158	6	44	theme	lipid	948:952	arg1	oxidation					954:962	lipid oxidation	948:962	lipid oxidation in emulsions	948:975	Meanwhile, the physical stability and lipid oxidation in emulsions were monitored.
29100158	2	45	theme	chemical	370:377	arg1	composition					379:389	their chemical composition	364:389	their chemical composition resulting from the isolation method	364:425	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	5	46	theme	storage	896:902	arg1	test					904:907	an accelerated storage test	881:907	an accelerated storage test	881:907	Distribution and changes occurring in the phenolic residues at the droplet interface and in the continuous phase were studied during an accelerated storage test.
29100158	4	47	theme	phenolic	615:622	arg1	residues					624:631	phenolic residues	615:631	phenolic residues	615:631	EXPERIMENTS We investigated the role of phenolic residues in spruce hemicellulose extracts in the stabilization of oil-in-water emulsions by physical and chemical approach.
29100158	8	48	theme	GGM	1221:1223	arg1	method					1211:1216	the isolation method	1197:1216	the isolation method of GGM regarding their phenolic profile	1197:1256	By adjusting the isolation method of GGM regarding their phenolic profile, their functionalities, especially interfacial behavior, can be altered.
29100158	9	49	theme	phenolic	1376:1383	arg1	residues					1385:1392	GGM and phenolic residues	1368:1392	GGM and phenolic residues	1368:1392	Retaining the native interactions of GGM and phenolic residues is suggested for efficient physical stabilization and extended protection against lipid oxidation.
29100158	9	50	theme	residues	1385:1392	arg1	interactions					1352:1363	the native interactions	1341:1363	the native interactions of GGM and phenolic residues	1341:1392	Retaining the native interactions of GGM and phenolic residues is suggested for efficient physical stabilization and extended protection against lipid oxidation.
29100158	7	51	theme	droplet	1110:1116	arg1	interface					1118:1126	the oil droplet interface	1102:1126	the oil droplet interface	1102:1126	FINDINGS Naturally associated lignin residues in GGM act as vehicles for anchoring these hemicelluloses into the oil droplet interface and further enable superior stabilization of emulsions.
29100158	9	52	theme	GGM	1368:1370	arg1	residues					1385:1392	GGM and phenolic residues	1368:1392	GGM and phenolic residues	1368:1392	Retaining the native interactions of GGM and phenolic residues is suggested for efficient physical stabilization and extended protection against lipid oxidation.
29100158	7	53	theme	lignin	1023:1028	arg1	residues					1030:1037	lignin residues	1023:1037	lignin residues in GGM	1023:1044	FINDINGS Naturally associated lignin residues in GGM act as vehicles for anchoring these hemicelluloses into the oil droplet interface and further enable superior stabilization of emulsions.
29100158	8	54	theme	phenolic	1241:1248	arg1	profile					1250:1256	their phenolic profile	1235:1256	their phenolic profile	1235:1256	By adjusting the isolation method of GGM regarding their phenolic profile, their functionalities, especially interfacial behavior, can be altered.
29100158	2	55	theme	wood	234:237	arg1	extracts					239:246	Hemicellulose-rich wood extracts	215:246	Hemicellulose-rich wood extracts	215:246	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	3	56	theme	interfacial	537:547	arg1	properties					549:558	emulsions' interfacial properties	526:558	emulsions' interfacial properties	526:558	Lignin-derived phenolic residues associated with hemicelluloses are hypothesized to contribute to emulsions' interfacial properties and stability.
29100158	2	57	contain	contain	248:254	arg2	assemblies					256:265	assemblies	256:265	assemblies (lignin-carbohydrate complexes, LCC)	256:302	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	2	57	contain	contain	248:254	arg1	extracts					239:246	Hemicellulose-rich wood extracts	215:246	Hemicellulose-rich wood extracts	215:246	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	5	58	theme	phenolic	790:797	arg1	residues					799:806	the phenolic residues	786:806	the phenolic residues at the droplet interface and in the continuous phase	786:859	Distribution and changes occurring in the phenolic residues at the droplet interface and in the continuous phase were studied during an accelerated storage test.
29100158	2	59	theme	Hemicellulose-rich	215:232	arg1	extracts					239:246	Hemicellulose-rich wood extracts	215:246	Hemicellulose-rich wood extracts	215:246	Hemicellulose-rich wood extracts contain assemblies (lignin-carbohydrate complexes, LCC) with natural amphiphilicity, which is expected to depend on their chemical composition resulting from the isolation method.
29100158	0	60	theme	emulsions	86:94	arg1	stabilization					56:68	stabilization	56:68	stabilization of oil-in-water emulsions	56:94	Phenolic residues in spruce galactoglucomannans improve stabilization of oil-in-water emulsions.
29100158	7	61	theme	oil	1106:1108	arg1	interface					1118:1126	the oil droplet interface	1102:1126	the oil droplet interface	1102:1126	FINDINGS Naturally associated lignin residues in GGM act as vehicles for anchoring these hemicelluloses into the oil droplet interface and further enable superior stabilization of emulsions.
29100158	0	62	theme	oil-in-water	73:84	arg1	emulsions					86:94	oil-in-water emulsions	73:94	oil-in-water emulsions	73:94	Phenolic residues in spruce galactoglucomannans improve stabilization of oil-in-water emulsions.
29100158	9	63	theme	physical	1421:1428	arg1	stabilization					1430:1442	efficient physical stabilization	1411:1442	efficient physical stabilization	1411:1442	Retaining the native interactions of GGM and phenolic residues is suggested for efficient physical stabilization and extended protection against lipid oxidation.
29100158	3	64	link	Lignin-derived	428:441	arg1	residues					452:459	Lignin-derived phenolic residues	428:459	Lignin-derived phenolic residues associated with hemicelluloses	428:490	Lignin-derived phenolic residues associated with hemicelluloses are hypothesized to contribute to emulsions' interfacial properties and stability.
29100158	4	65	theme	oil-in-water	690:701	arg1	emulsions					703:711	oil-in-water emulsions	690:711	oil-in-water emulsions	690:711	EXPERIMENTS We investigated the role of phenolic residues in spruce hemicellulose extracts in the stabilization of oil-in-water emulsions by physical and chemical approach.
29100158	4	66	theme	emulsions	703:711	arg1	stabilization					673:685	the stabilization	669:685	the stabilization of oil-in-water emulsions by physical and chemical approach	669:745	EXPERIMENTS We investigated the role of phenolic residues in spruce hemicellulose extracts in the stabilization of oil-in-water emulsions by physical and chemical approach.
28964522	2	0	theme	outdoor	442:448	arg1	grazing					450:456	outdoor grazing	442:456	outdoor grazing on perennial ryegrass	442:478	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	5	1	dep	June	910:913	arg1	to					918:919	to	918:919	to	918:919	Milk samples were collected on 10 occasions over the period June 17 to November 26, at 2 or 3 weekly intervals, when cows were on average 119 to 281 d in lactation (DIL).
28964522	5	1	dep	June	910:913	arg1	November					921:928	November	921:928	November	921:928	Milk samples were collected on 10 occasions over the period June 17 to November 26, at 2 or 3 weekly intervals, when cows were on average 119 to 281 d in lactation (DIL).
28964522	4	2	theme	feeding	807:813	arg1	systems					815:821	the 3 feeding systems	801:821	the 3 feeding systems from February to November	801:847	The herds were allocated to 1 of the 3 feeding systems from February to November.
28964522	10	3	theme	Ca	1675:1676	arg1	concentrations					1653:1666	concentrations	1653:1666	concentrations of TS, Ca, and P similar to those of TMR milk	1653:1712	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	10	3	theme	Ca	1675:1676	arg1	levels					1589:1594	levels	1589:1594	levels of lactose, Zn, and Cu similar to those of GRO milk	1589:1646	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	8	4	theme	solids	1427:1432	arg1	concentrations					1403:1416	the highest mean concentrations	1386:1416	the highest mean concentrations of total solids, total protein, casein, Ca, and P	1386:1466	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	1	5	theme	gross	221:225	arg1	composition					227:237	the gross composition	217:237	the gross composition	217:237	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	11	6	theme	Se	1805:1806	arg1	concentrations					1771:1784	the concentrations	1767:1784	the concentrations of Fe, Cu, Mn, and Se	1767:1806	Lactation period affected all variables, apart from the concentrations of Fe, Cu, Mn, and Se.
28964522	13	7	dep	composition	2045:2055	arg1	the					2035:2037	the	2035:2037	the	2035:2037	The results demonstrate how the gross composition and elemental composition of milk can be affected by different feeding systems.
28964522	9	8	contain	had	1482:1484	arg2	level					1548:1552	lowest level	1541:1552	lowest level of total protein	1541:1569	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	9	8	contain	had	1482:1484	arg1	milk					1477:1480	The TMR milk	1469:1480	The TMR milk	1469:1480	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	9	8	contain	had	1482:1484	arg2	concentrations					1498:1511	the highest concentrations	1486:1511	the highest concentrations of lactose, Cu, and Se	1486:1534	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	8	9	theme	total	1435:1439	arg1	solids					1427:1432	total solids	1421:1432	total solids	1421:1432	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	8	9	theme	total	1435:1439	arg1	protein					1441:1447	total protein	1435:1447	total protein	1435:1447	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	8	10	contain	had	1382:1384	arg1	milk					1377:1380	The GRO milk	1369:1380	The GRO milk	1369:1380	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	8	10	contain	had	1382:1384	arg2	concentrations					1403:1416	the highest mean concentrations	1386:1416	the highest mean concentrations of total solids, total protein, casein, Ca, and P	1386:1466	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	12	11	theme	total	2000:2004	arg1	%					1998:1998	≤45% total	1995:2004	≤45% total	1995:2004	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	5	12	dep	281	995:997	arg1	to					992:993	to	992:993	to	992:993	Milk samples were collected on 10 occasions over the period June 17 to November 26, at 2 or 3 weekly intervals, when cows were on average 119 to 281 d in lactation (DIL).
28964522	10	13	theme	Zn	1608:1609	arg1	concentrations					1653:1666	concentrations	1653:1666	concentrations of TS, Ca, and P similar to those of TMR milk	1653:1712	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	10	13	theme	Zn	1608:1609	arg1	levels					1589:1594	levels	1589:1594	levels of lactose, Zn, and Cu similar to those of GRO milk	1589:1646	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	7	14	theme	feeding	1353:1359	arg1	system					1361:1366	feeding system	1353:1366	feeding system	1353:1366	With the exception of Mg, Na, Fe, Mo, and Co, all other variables were affected by feeding system.
28964522	5	15	from	d	999:999	arg1	DIL					1015:1017	DIL	1015:1017	DIL	1015:1017	Milk samples were collected on 10 occasions over the period June 17 to November 26, at 2 or 3 weekly intervals, when cows were on average 119 to 281 d in lactation (DIL).
28964522	5	15	from	d	999:999	arg1	lactation					1004:1012	lactation	1004:1012	lactation (DIL)	1004:1018	Milk samples were collected on 10 occasions over the period June 17 to November 26, at 2 or 3 weekly intervals, when cows were on average 119 to 281 d in lactation (DIL).
28964522	10	16	theme	lactose	1599:1605	arg1	concentrations					1653:1666	concentrations	1653:1666	concentrations of TS, Ca, and P similar to those of TMR milk	1653:1712	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	10	16	theme	lactose	1599:1605	arg1	levels					1589:1594	levels	1589:1594	levels of lactose, Zn, and Cu similar to those of GRO milk	1589:1646	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	3	17	theme	Sixty	558:562	arg1	cows					603:606	Sixty spring-calving Holstein Friesian dairy cows	558:606	Sixty spring-calving Holstein Friesian dairy cows	558:606	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	13	18	theme	elemental	2061:2069	arg1	composition					2071:2081	elemental composition	2061:2081	elemental composition	2061:2081	The results demonstrate how the gross composition and elemental composition of milk can be affected by different feeding systems.
28964522	11	19	theme	Mn	1797:1798	arg1	concentrations					1771:1784	the concentrations	1767:1784	the concentrations of Fe, Cu, Mn, and Se	1767:1806	Lactation period affected all variables, apart from the concentrations of Fe, Cu, Mn, and Se.
28964522	9	20	theme	TMR	1473:1475	arg1	milk					1477:1480	The TMR milk	1469:1480	The TMR milk	1469:1480	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	6	21	theme	total	1025:1029	arg1	period					1041:1046	The total lactation period	1021:1046	The total lactation period	1021:1046	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	7	22	theme	Mg	1292:1293	arg1	exception					1279:1287	the exception	1275:1287	the exception of Mg, Na, Fe, Mo, and Co	1275:1313	With the exception of Mg, Na, Fe, Mo, and Co, all other variables were affected by feeding system.
28964522	2	23	theme	outdoor	389:395	arg1	grazing					397:403	outdoor grazing	389:403	outdoor grazing	389:403	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	11	24	theme	Lactation	1715:1723	arg1	period					1725:1730	Lactation period	1715:1730	Lactation period	1715:1730	Lactation period affected all variables, apart from the concentrations of Fe, Cu, Mn, and Se.
28964522	7	25	theme	other	1320:1324	arg1	variables					1326:1334	all other variables	1316:1334	all other variables	1316:1334	With the exception of Mg, Na, Fe, Mo, and Co, all other variables were affected by feeding system.
28964522	3	26	theme	dairy	597:601	arg1	cows					603:606	Sixty spring-calving Holstein Friesian dairy cows	558:606	Sixty spring-calving Holstein Friesian dairy cows	558:606	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	6	27	theme	mid	1122:1124	arg1	June					1137:1140	June	1137:1140	June	1137:1140	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	6	27	theme	mid	1122:1124	arg1	lactation					1126:1134	mid lactation	1122:1134	mid lactation	1122:1134	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	6	27	theme	mid	1122:1124	arg1	periods					1093:1099	2 lactation periods	1081:1099	2 lactation periods based on DIL	1081:1112	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	13	28	theme	feeding	2120:2126	arg1	systems					2128:2134	different feeding systems	2110:2134	different feeding systems	2110:2134	The results demonstrate how the gross composition and elemental composition of milk can be affected by different feeding systems.
28964522	1	29	theme	system	186:191	arg1	influence					165:173	The influence	161:173	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk	161:340	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	0	30	from	indoor	48:53	arg1	grazing					8:14	Outdoor grazing	0:14	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.	0:159	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	9	31	theme	highest	1490:1496	arg1	concentrations					1498:1511	the highest concentrations	1486:1511	the highest concentrations of lactose, Cu, and Se	1486:1534	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	12	32	theme	high-speed	1905:1914	arg1	ultracentrifugation					1916:1934	high-speed ultracentrifugation	1905:1934	high-speed ultracentrifugation	1905:1934	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	3	33	theme	Holstein	579:586	arg1	cows					603:606	Sixty spring-calving Holstein Friesian dairy cows	558:606	Sixty spring-calving Holstein Friesian dairy cows	558:606	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	0	34	theme	mineral	119:125	arg1	content					127:133	mineral content	119:133	mineral content of milk during lactation	119:158	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	1	35	from	influence	165:173	arg1	Fe					299:300	Fe	299:300	Fe	299:300	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	Mn					311:312	Mn	311:312	Mn	311:312	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	Mg					262:263	Mg	262:263	Mg	262:263	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	Se					315:316	Se	315:316	Se	315:316	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	macroelements					240:252	macroelements	240:252	macroelements (Ca, P, Mg, and Na)	240:272	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	elements					285:292	trace elements	279:292	trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co)	279:325	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	Cu					303:304	Cu	303:304	Cu	303:304	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	Ca					255:256	Ca	255:256	Ca	255:256	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	Mo					307:308	Mo	307:308	Mo	307:308	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	Na					270:271	Na	270:271	Na	270:271	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	Co					323:324	Co	323:324	Co	323:324	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	Zn					295:296	Zn	295:296	Zn	295:296	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	composition					227:237	the gross composition	217:237	the gross composition	217:237	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	35	from	influence	165:173	arg1	P					259:259	P	259:259	P	259:259	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	6	36	dep	September	1211:1219	arg1	to					1224:1225	to	1224:1225	to	1224:1225	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	6	36	dep	September	1211:1219	arg1	November					1227:1234	November	1227:1234	November	1227:1234	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	9	37	theme	total	1557:1561	arg1	protein					1563:1569	total protein	1557:1569	total protein	1557:1569	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	2	38	theme	ryegrass	418:425	arg1	GRO					436:438	GRO	436:438	GRO	436:438	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	2	38	theme	ryegrass	418:425	arg1	pasture					427:433	perennial ryegrass pasture	408:433	perennial ryegrass pasture (GRO)	408:439	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	2	39	from	pasture	497:503	arg1	ryegrass					471:478	perennial ryegrass	461:478	perennial ryegrass	461:478	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	5	40	theme	281	995:997	arg1	d					999:999	average 119 to 281 d	980:999	average 119 to 281 d in lactation (DIL)	980:1018	Milk samples were collected on 10 occasions over the period June 17 to November 26, at 2 or 3 weekly intervals, when cows were on average 119 to 281 d in lactation (DIL).
28964522	3	41	dep	each	634:637	arg1	balanced					666:673	balanced	666:673	balanced	666:673	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	3	41	dep	each	634:637	arg1	consisting					639:648	consisting	639:648	consisting of 20 cows	639:659	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	6	42	theme	lactation	1083:1091	arg1	lactation					1126:1134	mid lactation	1122:1134	mid lactation	1122:1134	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	6	42	theme	lactation	1083:1091	arg1	periods					1093:1099	2 lactation periods	1081:1099	2 lactation periods based on DIL	1081:1112	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	8	43	theme	GRO	1373:1375	arg1	milk					1377:1380	The GRO milk	1369:1380	The GRO milk	1369:1380	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	2	44	theme	mixed	538:542	arg1	ration					544:549	total mixed ration	532:549	total mixed ration (TMR)	532:555	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	2	44	theme	mixed	538:542	arg1	TMR					552:554	TMR	552:554	TMR	552:554	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	4	45	dep	February	828:835	arg1	November					840:847	November	840:847	November	840:847	The herds were allocated to 1 of the 3 feeding systems from February to November.
28964522	4	45	dep	February	828:835	arg1	to					837:838	to	837:838	to	837:838	The herds were allocated to 1 of the 3 feeding systems from February to November.
28964522	7	46	theme	Mo	1304:1305	arg1	exception					1279:1287	the exception	1275:1287	the exception of Mg, Na, Fe, Mo, and Co	1275:1313	With the exception of Mg, Na, Fe, Mo, and Co, all other variables were affected by feeding system.
28964522	2	47	theme	clover	490:495	arg1	GRC					506:508	GRC	506:508	GRC	506:508	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	2	47	theme	clover	490:495	arg1	pasture					497:503	white clover pasture	484:503	white clover pasture (GRC)	484:509	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	0	48	theme	mixed	72:76	arg1	ration					78:83	total mixed ration	66:83	total mixed ration	66:83	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	8	49	theme	highest	1390:1396	arg1	concentrations					1403:1416	the highest mean concentrations	1386:1416	the highest mean concentrations of total solids, total protein, casein, Ca, and P	1386:1466	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	7	50	theme	Fe	1300:1301	arg1	exception					1279:1287	the exception	1275:1287	the exception of Mg, Na, Fe, Mo, and Co	1275:1313	With the exception of Mg, Na, Fe, Mo, and Co, all other variables were affected by feeding system.
28964522	0	51	from	pasture	33:39	arg1	grazing					8:14	Outdoor grazing	0:14	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.	0:159	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	10	52	theme	TS	1671:1672	arg1	concentrations					1653:1666	concentrations	1653:1666	concentrations of TS, Ca, and P similar to those of TMR milk	1653:1712	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	10	52	theme	TS	1671:1672	arg1	levels					1589:1594	levels	1589:1594	levels of lactose, Zn, and Cu similar to those of GRO milk	1589:1646	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	5	53	theme	weekly	944:949	arg1	intervals					951:959	2 or 3 weekly intervals	937:959	2 or 3 weekly intervals	937:959	Milk samples were collected on 10 occasions over the period June 17 to November 26, at 2 or 3 weekly intervals, when cows were on average 119 to 281 d in lactation (DIL).
28964522	13	54	theme	gross	2039:2043	arg1	composition					2045:2055	gross composition	2039:2055	gross composition	2039:2055	The results demonstrate how the gross composition and elemental composition of milk can be affected by different feeding systems.
28964522	7	55	theme	Na	1296:1297	arg1	exception					1279:1287	the exception	1275:1287	the exception of Mg, Na, Fe, Mo, and Co	1275:1313	With the exception of Mg, Na, Fe, Mo, and Co, all other variables were affected by feeding system.
28964522	0	56	from	cows	25:28	arg1	indoor					48:53	indoor	48:53	indoor	48:53	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	0	56	from	cows	25:28	arg1	pasture					33:39	pasture	33:39	pasture	33:39	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	10	57	theme	similar	1685:1691	arg1	P					1683:1683	P	1683:1683	P similar to those of TMR milk	1683:1712	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	0	58	theme	Outdoor	0:6	arg1	grazing					8:14	Outdoor grazing	0:14	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.	0:159	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	8	59	theme	total	1421:1425	arg1	P					1466:1466	P	1466:1466	P	1466:1466	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	8	59	theme	total	1421:1425	arg1	casein					1450:1455	casein	1450:1455	casein	1450:1455	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	8	59	theme	total	1421:1425	arg1	solids					1427:1432	total solids	1421:1432	total solids	1421:1432	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	8	59	theme	total	1421:1425	arg1	protein					1441:1447	total protein	1435:1447	total protein	1435:1447	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	8	59	theme	total	1421:1425	arg1	Ca					1458:1459	Ca	1458:1459	Ca	1458:1459	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	2	60	dep	indoors	516:522	arg1	offered					524:530	offered	524:530	indoors offered total mixed ration (TMR)	516:555	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	6	61	dep	203	1182:1184	arg1	DIL					1186:1188	DIL	1186:1188	119 to 203 DIL	1175:1188	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	6	61	dep	203	1182:1184	arg1	to					1179:1180	to	1179:1180	to	1179:1180	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	12	62	from	%	1958:1958	arg1	average					1812:1818	average	1812:1818	average	1812:1818	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	0	63	theme	cows	25:28	arg1	grazing					8:14	Outdoor grazing	0:14	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.	0:159	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	2	64	from	grazing	450:456	arg1	ryegrass					471:478	perennial ryegrass	461:478	perennial ryegrass	461:478	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	1	65	theme	milk	337:340	arg1	Fe					299:300	Fe	299:300	Fe	299:300	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	Mn					311:312	Mn	311:312	Mn	311:312	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	Mg					262:263	Mg	262:263	Mg	262:263	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	Se					315:316	Se	315:316	Se	315:316	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	macroelements					240:252	macroelements	240:252	macroelements (Ca, P, Mg, and Na)	240:272	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	elements					285:292	trace elements	279:292	trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co)	279:325	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	Cu					303:304	Cu	303:304	Cu	303:304	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	Ca					255:256	Ca	255:256	Ca	255:256	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	Mo					307:308	Mo	307:308	Mo	307:308	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	Na					270:271	Na	270:271	Na	270:271	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	Co					323:324	Co	323:324	Co	323:324	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	Zn					295:296	Zn	295:296	Zn	295:296	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	composition					227:237	the gross composition	217:237	the gross composition	217:237	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	65	theme	milk	337:340	arg1	P					259:259	P	259:259	P	259:259	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	3	66	theme	milk	734:737	arg1	yield					739:743	pre-experimental milk yield	717:743	pre-experimental milk yield	717:743	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	10	67	theme	GRC	1576:1578	arg1	milk					1580:1583	The GRC milk	1572:1583	The GRC milk	1572:1583	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	10	68	contain	had	1585:1587	arg2	levels					1589:1594	levels	1589:1594	levels of lactose, Zn, and Cu similar to those of GRO milk	1589:1646	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	10	68	contain	had	1585:1587	arg1	milk					1580:1583	The GRC milk	1572:1583	The GRC milk	1572:1583	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	10	68	contain	had	1585:1587	arg2	concentrations					1653:1666	concentrations	1653:1666	concentrations of TS, Ca, and P similar to those of TMR milk	1653:1712	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	1	69	theme	period	207:212	arg1	influence					165:173	The influence	161:173	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk	161:340	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	6	70	dep	281	1261:1263	arg1	to					1258:1259	to	1258:1259	to	1258:1259	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	3	71	theme	solids	754:759	arg1	yield					761:765	milk solids yield	749:765	milk solids yield	749:765	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	4	72	theme	systems	815:821	arg1	systems					815:821	the 3 feeding systems	801:821	the 3 feeding systems from February to November	801:847	The herds were allocated to 1 of the 3 feeding systems from February to November.
28964522	4	72	theme	systems	815:821	arg1	1					796:796	1	796:796	1	796:796	The herds were allocated to 1 of the 3 feeding systems from February to November.
28964522	10	73	theme	TMR	1705:1707	arg1	milk					1709:1712	TMR milk	1705:1712	TMR milk	1705:1712	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	6	74	dep	June	1137:1140	arg1	to					1145:1146	to	1145:1146	to	1145:1146	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	6	74	dep	June	1137:1140	arg1	September					1148:1156	September	1148:1156	September	1148:1156	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	12	75	theme	P	1853:1853	arg1	proportion					1825:1834	the proportion	1821:1834	the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g	1821:1949	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	12	75	theme	P	1853:1853	arg1	%					1958:1958	≥60%	1955:1958	≥60%	1955:1958	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	12	75	theme	P	1853:1853	arg1	%					1837:1837	%	1837:1837	%	1837:1837	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	2	76	from	pasture	427:433	arg1	ryegrass					471:478	perennial ryegrass	461:478	perennial ryegrass	461:478	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	6	77	theme	late	1195:1198	arg1	lactation					1200:1208	late lactation	1195:1208	late lactation	1195:1208	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	2	78	theme	perennial	461:469	arg1	ryegrass					471:478	perennial ryegrass	461:478	perennial ryegrass	461:478	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	12	79	theme	Zn	1856:1857	arg1	proportion					1825:1834	the proportion	1821:1834	the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g	1821:1949	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	12	79	theme	Zn	1856:1857	arg1	%					1958:1958	≥60%	1955:1958	≥60%	1955:1958	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	12	79	theme	Zn	1856:1857	arg1	%					1837:1837	%	1837:1837	%	1837:1837	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	13	80	theme	milk	2086:2089	arg1	composition					2045:2055	gross composition	2039:2055	gross composition	2039:2055	The results demonstrate how the gross composition and elemental composition of milk can be affected by different feeding systems.
28964522	13	80	theme	milk	2086:2089	arg1	composition					2071:2081	elemental composition	2061:2081	elemental composition	2061:2081	The results demonstrate how the gross composition and elemental composition of milk can be affected by different feeding systems.
28964522	1	81	dep	macroelements	240:252	arg1	Ca					255:256	Ca	255:256	Ca	255:256	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	81	dep	macroelements	240:252	arg1	macroelements					240:252	macroelements	240:252	macroelements (Ca, P, Mg, and Na)	240:272	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	81	dep	macroelements	240:252	arg1	Mg					262:263	Mg	262:263	Mg	262:263	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	81	dep	macroelements	240:252	arg1	Na					270:271	Na	270:271	Na	270:271	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	81	dep	macroelements	240:252	arg1	P					259:259	P	259:259	P	259:259	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	10	82	theme	GRO	1639:1641	arg1	milk					1643:1646	GRO milk	1639:1646	GRO milk	1639:1646	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	3	83	theme	spring-calving	564:577	arg1	cows					603:606	Sixty spring-calving Holstein Friesian dairy cows	558:606	Sixty spring-calving Holstein Friesian dairy cows	558:606	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	6	84	theme	lactation	1031:1039	arg1	period					1041:1046	The total lactation period	1021:1046	The total lactation period	1021:1046	The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL.
28964522	10	85	theme	Cu	1616:1617	arg1	concentrations					1653:1666	concentrations	1653:1666	concentrations of TS, Ca, and P similar to those of TMR milk	1653:1712	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	10	85	theme	Cu	1616:1617	arg1	levels					1589:1594	levels	1589:1594	levels of lactose, Zn, and Cu similar to those of GRO milk	1589:1646	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	1	86	theme	feeding	178:184	arg1	system					186:191	feeding system	178:191	feeding system	178:191	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	2	87	theme	total	532:536	arg1	ration					544:549	total mixed ration	532:549	total mixed ration (TMR)	532:555	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	2	87	theme	total	532:536	arg1	TMR					552:554	TMR	552:554	TMR	552:554	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	5	88	theme	Milk	850:853	arg1	samples					855:861	Milk samples	850:861	Milk samples	850:861	Milk samples were collected on 10 occasions over the period June 17 to November 26, at 2 or 3 weekly intervals, when cows were on average 119 to 281 d in lactation (DIL).
28964522	0	89	theme	gross	97:101	arg1	composition					103:113	gross composition	97:113	gross composition	97:113	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	0	90	from	Effects	86:92	arg1	composition					103:113	gross composition	97:113	gross composition	97:113	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	0	90	from	Effects	86:92	arg1	content					127:133	mineral content	119:133	mineral content of milk during lactation	119:158	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	3	91	theme	Friesian	588:595	arg1	cows					603:606	Sixty spring-calving Holstein Friesian dairy cows	558:606	Sixty spring-calving Holstein Friesian dairy cows	558:606	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	0	92	theme	milk	138:141	arg1	composition					103:113	gross composition	97:113	gross composition	97:113	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	0	92	theme	milk	138:141	arg1	content					127:133	mineral content	119:133	mineral content of milk during lactation	119:158	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	9	93	theme	Se	1533:1534	arg1	concentrations					1498:1511	the highest concentrations	1486:1511	the highest concentrations of lactose, Cu, and Se	1486:1534	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	9	93	theme	Se	1533:1534	arg1	level					1548:1552	lowest level	1541:1552	lowest level of total protein	1541:1569	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	2	94	theme	perennial	408:416	arg1	GRO					436:438	GRO	436:438	GRO	436:438	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	2	94	theme	perennial	408:416	arg1	pasture					427:433	perennial ryegrass pasture	408:433	perennial ryegrass pasture (GRO)	408:439	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	9	95	theme	lactose	1516:1522	arg1	concentrations					1498:1511	the highest concentrations	1486:1511	the highest concentrations of lactose, Cu, and Se	1486:1534	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	9	95	theme	lactose	1516:1522	arg1	level					1548:1552	lowest level	1541:1552	lowest level of total protein	1541:1569	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	10	96	theme	similar	1619:1625	arg1	lactose					1599:1605	lactose	1599:1605	lactose	1599:1605	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	4	97	from	February	828:835	arg1	systems					815:821	the 3 feeding systems	801:821	the 3 feeding systems from February to November	801:847	The herds were allocated to 1 of the 3 feeding systems from February to November.
28964522	9	98	theme	Cu	1525:1526	arg1	concentrations					1498:1511	the highest concentrations	1486:1511	the highest concentrations of lactose, Cu, and Se	1486:1534	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	9	98	theme	Cu	1525:1526	arg1	level					1548:1552	lowest level	1541:1552	lowest level of total protein	1541:1569	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	0	99	theme	total	66:70	arg1	ration					78:83	total mixed ration	66:83	total mixed ration	66:83	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	13	100	theme	different	2110:2118	arg1	systems					2128:2134	different feeding systems	2110:2134	different feeding systems	2110:2134	The results demonstrate how the gross composition and elemental composition of milk can be affected by different feeding systems.
28964522	7	101	theme	Co	1312:1313	arg1	exception					1279:1287	the exception	1275:1287	the exception of Mg, Na, Fe, Mo, and Co	1275:1313	With the exception of Mg, Na, Fe, Mo, and Co, all other variables were affected by feeding system.
28964522	0	102	from	grazing	8:14	arg1	indoor					48:53	indoor	48:53	indoor	48:53	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	0	102	from	grazing	8:14	arg1	pasture					33:39	pasture	33:39	pasture	33:39	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	11	103	theme	Cu	1793:1794	arg1	concentrations					1771:1784	the concentrations	1767:1784	the concentrations of Fe, Cu, Mn, and Se	1767:1806	Lactation period affected all variables, apart from the concentrations of Fe, Cu, Mn, and Se.
28964522	5	104	theme	average	980:986	arg1	d					999:999	average 119 to 281 d	980:999	average 119 to 281 d in lactation (DIL)	980:1018	Milk samples were collected on 10 occasions over the period June 17 to November 26, at 2 or 3 weekly intervals, when cows were on average 119 to 281 d in lactation (DIL).
28964522	11	105	theme	Fe	1789:1790	arg1	concentrations					1771:1784	the concentrations	1767:1784	the concentrations of Fe, Cu, Mn, and Se	1767:1806	Lactation period affected all variables, apart from the concentrations of Fe, Cu, Mn, and Se.
28964522	2	106	theme	feeding	364:370	arg1	systems					372:378	The feeding systems	360:378	The feeding systems	360:378	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	2	107	theme	white	484:488	arg1	GRC					506:508	GRC	506:508	GRC	506:508	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	2	107	theme	white	484:488	arg1	pasture					497:503	white clover pasture	484:503	white clover pasture (GRC)	484:509	The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR).
28964522	12	108	theme	Ca	1849:1850	arg1	proportion					1825:1834	the proportion	1821:1834	the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g	1821:1949	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	12	108	theme	Ca	1849:1850	arg1	%					1958:1958	≥60%	1955:1958	≥60%	1955:1958	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	12	108	theme	Ca	1849:1850	arg1	%					1837:1837	%	1837:1837	%	1837:1837	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	1	109	theme	trace	279:283	arg1	Fe					299:300	Fe	299:300	Fe	299:300	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	109	theme	trace	279:283	arg1	Mn					311:312	Mn	311:312	Mn	311:312	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	109	theme	trace	279:283	arg1	Se					315:316	Se	315:316	Se	315:316	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	109	theme	trace	279:283	arg1	elements					285:292	trace elements	279:292	trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co)	279:325	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	109	theme	trace	279:283	arg1	Cu					303:304	Cu	303:304	Cu	303:304	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	109	theme	trace	279:283	arg1	Mo					307:308	Mo	307:308	Mo	307:308	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	109	theme	trace	279:283	arg1	Co					323:324	Co	323:324	Co	323:324	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	109	theme	trace	279:283	arg1	Zn					295:296	Zn	295:296	Zn	295:296	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	3	110	theme	calving	699:705	arg1	date					707:710	calving date	699:710	calving date	699:710	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	5	111	dep	period	903:908	arg1	June					910:913	June	910:913	June	910:913	Milk samples were collected on 10 occasions over the period June 17 to November 26, at 2 or 3 weekly intervals, when cows were on average 119 to 281 d in lactation (DIL).
28964522	9	112	theme	lowest	1541:1546	arg1	level					1548:1552	lowest level	1541:1552	lowest level of total protein	1541:1569	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	8	113	theme	mean	1398:1401	arg1	concentrations					1403:1416	the highest mean concentrations	1386:1416	the highest mean concentrations of total solids, total protein, casein, Ca, and P	1386:1466	The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P.
28964522	10	114	theme	P	1683:1683	arg1	concentrations					1653:1666	concentrations	1653:1666	concentrations of TS, Ca, and P similar to those of TMR milk	1653:1712	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	10	114	theme	P	1683:1683	arg1	levels					1589:1594	levels	1589:1594	levels of lactose, Zn, and Cu similar to those of GRO milk	1589:1646	The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk.
28964522	0	115	theme	dairy	19:23	arg1	cows					25:28	dairy cows	19:28	dairy cows on pasture versus indoor feeding on total mixed ration	19:83	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	12	116	theme	100,000	1939:1945	arg1	g					1949:1949	100,000 × g	1939:1949	100,000 × g	1939:1949	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	3	117	theme	pre-experimental	717:732	arg1	yield					739:743	pre-experimental milk yield	717:743	pre-experimental milk yield	717:743	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	9	118	theme	protein	1563:1569	arg1	concentrations					1498:1511	the highest concentrations	1486:1511	the highest concentrations of lactose, Cu, and Se	1486:1534	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	9	118	theme	protein	1563:1569	arg1	level					1548:1552	lowest level	1541:1552	lowest level of total protein	1541:1569	The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein.
28964522	12	119	theme	total	1843:1847	arg1	Ca					1849:1850	total Ca	1843:1850	total Ca	1843:1850	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	12	120	from	casein	1895:1900	arg1	ultracentrifugation					1916:1934	high-speed ultracentrifugation	1905:1934	high-speed ultracentrifugation	1905:1934	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	1	121	dep	elements	285:292	arg1	Fe					299:300	Fe	299:300	Fe	299:300	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	121	dep	elements	285:292	arg1	Mn					311:312	Mn	311:312	Mn	311:312	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	121	dep	elements	285:292	arg1	Se					315:316	Se	315:316	Se	315:316	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	121	dep	elements	285:292	arg1	elements					285:292	trace elements	279:292	trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co)	279:325	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	121	dep	elements	285:292	arg1	Cu					303:304	Cu	303:304	Cu	303:304	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	121	dep	elements	285:292	arg1	Mo					307:308	Mo	307:308	Mo	307:308	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	121	dep	elements	285:292	arg1	Co					323:324	Co	323:324	Co	323:324	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	121	dep	elements	285:292	arg1	Zn					295:296	Zn	295:296	Zn	295:296	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	1	122	theme	bovine	330:335	arg1	milk					337:340	bovine milk	330:340	bovine milk	330:340	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	3	123	theme	milk	749:752	arg1	yield					761:765	milk solids yield	749:765	milk solids yield	749:765	Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield.
28964522	12	124	theme	Se	1867:1868	arg1	proportion					1825:1834	the proportion	1821:1834	the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g	1821:1949	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	12	124	theme	Se	1867:1868	arg1	%					1958:1958	≥60%	1955:1958	≥60%	1955:1958	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	12	124	theme	Se	1867:1868	arg1	%					1837:1837	%	1837:1837	%	1837:1837	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	1	125	theme	lactation	197:205	arg1	period					207:212	lactation period	197:212	lactation period	197:212	The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated.
28964522	12	126	theme	×	1947:1947	arg1	g					1949:1949	100,000 × g	1939:1949	100,000 × g	1939:1949	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	0	127	dep	grazing	8:14	arg1	Effects					86:92	Effects	86:92	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.	0:159	Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation.
28964522	12	128	theme	Mn	1860:1861	arg1	proportion					1825:1834	the proportion	1821:1834	the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g	1821:1949	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	12	128	theme	Mn	1860:1861	arg1	%					1958:1958	≥60%	1955:1958	≥60%	1955:1958	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
28964522	12	128	theme	Mn	1860:1861	arg1	%					1837:1837	%	1837:1837	%	1837:1837	On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total.
27039355	7	0	theme	@	1556:1556	arg1	DMF					1557:1559	the CdHgTe@DMF	1546:1559	the CdHgTe@DMF	1546:1559	It can be implied that the CdHgTe@DMF may be an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy.
27039355	7	0	theme	@	1556:1556	arg1	tool					1595:1598	an effective multifunction tool	1568:1598	an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy	1568:1659	It can be implied that the CdHgTe@DMF may be an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy.
27039355	1	1	theme	probe	431:435	arg1	function					437:444	the fluorescent probe function	415:444	the fluorescent probe function of quantum dots	415:460	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	2	theme	dextran-magnetic	283:298	arg1	DMF					340:342	DMF	340:342	DMF	340:342	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	2	theme	dextran-magnetic	283:298	arg1	"					337:337	"dextran-magnetic layered double hydroxide-fluorouracil"	282:337	"dextran-magnetic layered double hydroxide-fluorouracil" (DMF)	282:343	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	6	3	theme	cell	1325:1328	arg1	effect					1338:1343	the cell imaging effect	1321:1343	the cell imaging effect	1321:1343	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	1	4	theme	dots	457:460	arg1	effect					525:530	slow-release curative effect	503:530	slow-release curative effect	503:530	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	4	theme	dots	457:460	arg1	function					437:444	the fluorescent probe function	415:444	the fluorescent probe function of quantum dots	415:460	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	4	theme	dots	457:460	arg1	transport					489:497	the magnetic targeting transport	466:497	the magnetic targeting transport	466:497	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	5	theme	CdHgTe	205:210	arg1	dots					197:200	a water-soluble near-infrared quantum dots	159:200	a water-soluble near-infrared quantum dots of CdHgTe	159:210	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	5	6	theme	@	1121:1121	arg1	composite					1126:1134	The CdHgTe@DMF composite	1111:1134	The CdHgTe@DMF composite	1111:1134	The CdHgTe@DMF composite showed super-paramagnetic and photoluminescence property in the near-infrared wavelength range of 575-780 nm.
27039355	6	7	theme	magnetic	1385:1392	arg1	field					1394:1398	the magnetic field	1381:1398	the magnetic field intensity	1381:1408	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	1	8	theme	DMF	401:403	arg1	form					386:389	form	386:389	form	386:389	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	2	9	theme	property	588:595	arg1	size					606:609	The luminescent property particle size	572:609	The luminescent property particle size	572:609	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	2	10	theme	laser	740:744	arg1	distribution					760:771	laser particle size distribution	740:771	laser particle size distribution	740:771	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	3	11	theme	targeting	893:901	arg1	performance					903:913	magnetic targeting performance	884:913	magnetic targeting performance of the multifunctional platform	884:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	11	theme	targeting	893:901	arg1	tags					868:871	fluorescent tags	856:871	fluorescent tags effect and magnetic targeting performance of the multifunctional platform	856:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	6	12	theme	@	1278:1278	arg1	composite					1283:1291	the CdHgTe@DMF composite	1268:1291	the CdHgTe@DMF composite	1268:1291	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	3	13	theme	experimental	834:845	arg1	study					847:851	The experimental study	830:851	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform	830:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	14	dep	tags	868:871	arg1	performance					903:913	magnetic targeting performance	884:913	magnetic targeting performance of the multifunctional platform	884:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	14	dep	tags	868:871	arg1	tags					868:871	fluorescent tags	856:871	fluorescent tags effect and magnetic targeting performance of the multifunctional platform	856:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	14	dep	tags	868:871	arg1	effect					873:878	effect	873:878	effect	873:878	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	6	15	theme	1.758 +	1454:1460	arg1	0.0075M					1462:1468	the linear relationship Dmean = 1.758 + 0.0075M	1422:1468	the linear relationship Dmean = 1.758 + 0.0075M	1422:1468	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	6	16	theme	magnetic	1494:1501	arg1	interference					1509:1520	magnetic field interference	1494:1520	magnetic field interference	1494:1520	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	3	17	theme	multifunctional	922:936	arg1	platform					938:945	the multifunctional platform	918:945	the multifunctional platform	918:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	7	18	theme	targeted	1644:1651	arg1	therapy					1653:1659	magnetic targeted therapy	1635:1659	magnetic targeted therapy	1635:1659	It can be implied that the CdHgTe@DMF may be an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy.
27039355	2	19	theme	size	755:758	arg1	distribution					760:771	laser particle size distribution	740:771	laser particle size distribution	740:771	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	0	20	theme	CdHgTe-Dextran-Magnetic	68:90	arg1	Hydroxide-Fluorouracil					107:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite.
27039355	1	21	theme	new	356:358	arg1	platform					374:381	a new nanostructure platform	354:381	a new nanostructure platform	354:381	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	22	theme	near-infrared	175:187	arg1	dots					197:200	a water-soluble near-infrared quantum dots	159:200	a water-soluble near-infrared quantum dots of CdHgTe	159:210	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	0	23	theme	Double	100:105	arg1	Hydroxide-Fluorouracil					107:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite.
27039355	5	24	theme	super-paramagnetic	1143:1160	arg1	property					1184:1191	super-paramagnetic and photoluminescence property	1143:1191	super-paramagnetic and photoluminescence property	1143:1191	The CdHgTe@DMF composite showed super-paramagnetic and photoluminescence property in the near-infrared wavelength range of 575-780 nm.
27039355	7	25	theme	optical	1612:1618	arg1	bioimaging					1620:1629	optical bioimaging	1612:1629	optical bioimaging	1612:1629	It can be implied that the CdHgTe@DMF may be an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy.
27039355	2	26	theme	composite	642:650	arg1	structure					625:633	internal structure	616:633	internal structure	616:633	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	2	26	theme	composite	642:650	arg1	size					606:609	The luminescent property particle size	572:609	The luminescent property particle size	572:609	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	5	27	theme	photoluminescence	1166:1182	arg1	property					1184:1191	super-paramagnetic and photoluminescence property	1143:1191	super-paramagnetic and photoluminescence property	1143:1191	The CdHgTe@DMF composite showed super-paramagnetic and photoluminescence property in the near-infrared wavelength range of 575-780 nm.
27039355	4	28	theme	DMF	1080:1082	arg1	surface					1069:1075	the surface	1065:1075	the surface of DMF	1065:1082	The results showed that the CdHgTe could be grafted successfully onto the surface of DMF by electrostatic coupling.
27039355	6	29	theme	interference	1509:1520	arg1	conditions					1480:1489	the conditions	1476:1489	the conditions of magnetic field interference	1476:1520	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	1	30	theme	layered	300:306	arg1	DMF					340:342	DMF	340:342	DMF	340:342	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	30	theme	layered	300:306	arg1	"					337:337	"dextran-magnetic layered double hydroxide-fluorouracil"	282:337	"dextran-magnetic layered double hydroxide-fluorouracil" (DMF)	282:343	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	31	theme	fluorescent	419:429	arg1	probe					431:435	the fluorescent probe	415:435	the fluorescent probe function of quantum dots	415:460	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	4	32	theme	electrostatic	1087:1099	arg1	coupling					1101:1108	electrostatic coupling	1087:1108	electrostatic coupling	1087:1108	The results showed that the CdHgTe could be grafted successfully onto the surface of DMF by electrostatic coupling.
27039355	2	33	theme	fluorescence	677:688	arg1	spectrophotometer					690:706	fluorescence spectrophotometer	677:706	fluorescence spectrophotometer	677:706	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	0	34	theme	Cell	14:17	arg1	Imaging					19:25	Cell Imaging	14:25	Cell Imaging	14:25	Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite.
27039355	1	35	theme	hydroxide-fluorouracil	315:336	arg1	DMF					340:342	DMF	340:342	DMF	340:342	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	35	theme	hydroxide-fluorouracil	315:336	arg1	"					337:337	"dextran-magnetic layered double hydroxide-fluorouracil"	282:337	"dextran-magnetic layered double hydroxide-fluorouracil" (DMF)	282:343	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	3	36	theme	confocal	977:984	arg1	imaging					986:992	fluorescent confocal imaging	965:992	fluorescent confocal imaging	965:992	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	7	37	theme	effective	1571:1579	arg1	DMF					1557:1559	the CdHgTe@DMF	1546:1559	the CdHgTe@DMF	1546:1559	It can be implied that the CdHgTe@DMF may be an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy.
27039355	7	37	theme	effective	1571:1579	arg1	tool					1595:1598	an effective multifunction tool	1568:1598	an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy	1568:1659	It can be implied that the CdHgTe@DMF may be an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy.
27039355	2	38	dep	Fourier	802:808	arg1	transform					810:818	transform	810:818	transform infrared	810:827	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	0	39	theme	Magnetic	58:65	arg1	Hydroxide-Fluorouracil					107:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite.
27039355	0	40	theme	Near-Infrared	32:44	arg1	Hydroxide-Fluorouracil					107:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite.
27039355	1	41	theme	slow-release	503:514	arg1	effect					525:530	slow-release curative effect	503:530	slow-release curative effect	503:530	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	7	42	theme	CdHgTe	1550:1555	arg1	DMF					1557:1559	the CdHgTe@DMF	1546:1559	the CdHgTe@DMF	1546:1559	It can be implied that the CdHgTe@DMF may be an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy.
27039355	7	42	theme	CdHgTe	1550:1555	arg1	tool					1595:1598	an effective multifunction tool	1568:1598	an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy	1568:1659	It can be implied that the CdHgTe@DMF may be an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy.
27039355	6	43	theme	Dmean =	1446:1452	arg1	0.0075M					1462:1468	the linear relationship Dmean = 1.758 + 0.0075M	1422:1468	the linear relationship Dmean = 1.758 + 0.0075M	1422:1468	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	6	44	theme	label	1350:1354	arg1	intensity					1356:1364	the label intensity	1346:1364	the label intensity	1346:1364	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	1	45	theme	DMF	535:537	arg1	effect					525:530	slow-release curative effect	503:530	slow-release curative effect	503:530	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	45	theme	DMF	535:537	arg1	function					437:444	the fluorescent probe function	415:444	the fluorescent probe function of quantum dots	415:460	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	45	theme	DMF	535:537	arg1	transport					489:497	the magnetic targeting transport	466:497	the magnetic targeting transport	466:497	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	46	theme	delivery	266:273	arg1	system					275:280	the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together	257:569	the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together	257:569	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	2	47	theme	particle	597:604	arg1	size					606:609	The luminescent property particle size	572:609	The luminescent property particle size	572:609	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	6	48	theme	imaging	1330:1336	arg1	effect					1338:1343	the cell imaging effect	1321:1343	the cell imaging effect	1321:1343	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	1	49	theme	quantum	449:455	arg1	dots					457:460	quantum dots	449:460	quantum dots	449:460	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	50	theme	quantum	189:195	arg1	dots					197:200	a water-soluble near-infrared quantum dots	159:200	a water-soluble near-infrared quantum dots of CdHgTe	159:210	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	2	51	theme	X-ray	779:783	arg1	diffraction					785:795	X-ray diffraction	779:795	X-ray diffraction	779:795	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	6	52	theme	relationship	1433:1444	arg1	0.0075M					1462:1468	the linear relationship Dmean = 1.758 + 0.0075M	1422:1468	the linear relationship Dmean = 1.758 + 0.0075M	1422:1468	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	5	53	theme	near-infrared	1200:1212	arg1	range					1225:1229	the near-infrared wavelength range	1196:1229	the near-infrared wavelength range of 575-780 nm	1196:1243	The CdHgTe@DMF composite showed super-paramagnetic and photoluminescence property in the near-infrared wavelength range of 575-780 nm.
27039355	2	54	theme	internal	616:623	arg1	structure					625:633	internal structure	616:633	internal structure	616:633	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	1	55	theme	magnetic	470:477	arg1	transport					489:497	the magnetic targeting transport	466:497	the magnetic targeting transport	466:497	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	6	56	theme	field	1394:1398	arg1	intensity					1400:1408	the magnetic field intensity	1381:1408	the magnetic field intensity	1381:1408	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	5	57	theme	DMF	1122:1124	arg1	composite					1126:1134	The CdHgTe@DMF composite	1111:1134	The CdHgTe@DMF composite	1111:1134	The CdHgTe@DMF composite showed super-paramagnetic and photoluminescence property in the near-infrared wavelength range of 575-780 nm.
27039355	2	58	theme	particle	746:753	arg1	distribution					760:771	laser particle size distribution	740:771	laser particle size distribution	740:771	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	6	59	theme	linear	1426:1431	arg1	0.0075M					1462:1468	the linear relationship Dmean = 1.758 + 0.0075M	1422:1468	the linear relationship Dmean = 1.758 + 0.0075M	1422:1468	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	5	60	theme	wavelength	1214:1223	arg1	range					1225:1229	the near-infrared wavelength range	1196:1229	the near-infrared wavelength range of 575-780 nm	1196:1243	The CdHgTe@DMF composite showed super-paramagnetic and photoluminescence property in the near-infrared wavelength range of 575-780 nm.
27039355	2	61	theme	luminescent	576:586	arg1	size					606:609	The luminescent property particle size	572:609	The luminescent property particle size	572:609	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	6	62	theme	DMF	1279:1281	arg1	composite					1283:1291	the CdHgTe@DMF composite	1268:1291	the CdHgTe@DMF composite	1268:1291	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	6	63	theme	CdHgTe	1272:1277	arg1	composite					1283:1291	the CdHgTe@DMF composite	1268:1291	the CdHgTe@DMF composite	1268:1291	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	5	64	theme	CdHgTe	1115:1120	arg1	composite					1126:1134	The CdHgTe@DMF composite	1111:1134	The CdHgTe@DMF composite	1111:1134	The CdHgTe@DMF composite showed super-paramagnetic and photoluminescence property in the near-infrared wavelength range of 575-780 nm.
27039355	3	65	theme	magnetic	884:891	arg1	performance					903:913	magnetic targeting performance	884:913	magnetic targeting performance of the multifunctional platform	884:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	65	theme	magnetic	884:891	arg1	tags					868:871	fluorescent tags	856:871	fluorescent tags effect and magnetic targeting performance of the multifunctional platform	856:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	7	66	theme	magnetic	1635:1642	arg1	therapy					1653:1659	magnetic targeted therapy	1635:1659	magnetic targeted therapy	1635:1659	It can be implied that the CdHgTe@DMF may be an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy.
27039355	0	67	theme	Layered	92:98	arg1	Hydroxide-Fluorouracil					107:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite.
27039355	1	68	theme	water-soluble	161:173	arg1	dots					197:200	a water-soluble near-infrared quantum dots	159:200	a water-soluble near-infrared quantum dots of CdHgTe	159:210	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	3	69	theme	platform	938:945	arg1	performance					903:913	magnetic targeting performance	884:913	magnetic targeting performance of the multifunctional platform	884:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	69	theme	platform	938:945	arg1	tags					868:871	fluorescent tags	856:871	fluorescent tags effect and magnetic targeting performance of the multifunctional platform	856:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	69	theme	platform	938:945	arg1	effect					873:878	effect	873:878	effect	873:878	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	1	70	theme	CdHgTe	394:399	arg1	DMF					401:403	CdHgTe@DMF	394:403	CdHgTe@DMF	394:403	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	0	71	theme	Hydroxide-Fluorouracil	107:128	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite.
27039355	0	71	theme	Hydroxide-Fluorouracil	107:128	arg1	Imaging					19:25	Cell Imaging	14:25	Cell Imaging	14:25	Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite.
27039355	1	72	theme	nanostructure	360:372	arg1	platform					374:381	a new nanostructure platform	354:381	a new nanostructure platform	354:381	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	3	73	theme	fluorescent	856:866	arg1	performance					903:913	magnetic targeting performance	884:913	magnetic targeting performance of the multifunctional platform	884:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	73	theme	fluorescent	856:866	arg1	tags					868:871	fluorescent tags	856:871	fluorescent tags effect and magnetic targeting performance of the multifunctional platform	856:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	73	theme	fluorescent	856:866	arg1	effect					873:878	effect	873:878	effect	873:878	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	74	theme	fluorescent	965:975	arg1	imaging					986:992	fluorescent confocal imaging	965:992	fluorescent confocal imaging	965:992	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	2	75	theme	ultraviolet	709:719	arg1	spectrophotometer					721:737	ultraviolet spectrophotometer	709:737	ultraviolet spectrophotometer	709:737	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	1	76	theme	@	400:400	arg1	DMF					401:403	CdHgTe@DMF	394:403	CdHgTe@DMF	394:403	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	6	77	theme	field	1503:1507	arg1	interference					1509:1520	magnetic field interference	1494:1520	magnetic field interference	1494:1520	Compared with CdHgTe, the CdHgTe@DMF composite could significantly improve the cell imaging effect, the label intensity increased with the magnetic field intensity, and obeyed the linear relationship Dmean = 1.758 + 0.0075M under the conditions of magnetic field interference.
27039355	1	78	theme	double	308:313	arg1	DMF					340:342	DMF	340:342	DMF	340:342	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	78	theme	double	308:313	arg1	"					337:337	"dextran-magnetic layered double hydroxide-fluorouracil"	282:337	"dextran-magnetic layered double hydroxide-fluorouracil" (DMF)	282:343	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	79	theme	targeting	479:487	arg1	transport					489:497	the magnetic targeting transport	466:497	the magnetic targeting transport	466:497	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	0	80	theme	Fluorescent	46:56	arg1	Hydroxide-Fluorouracil					107:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil	30:128	Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite.
27039355	7	81	theme	multifunction	1581:1593	arg1	DMF					1557:1559	the CdHgTe@DMF	1546:1559	the CdHgTe@DMF	1546:1559	It can be implied that the CdHgTe@DMF may be an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy.
27039355	7	81	theme	multifunction	1581:1593	arg1	tool					1595:1598	an effective multifunction tool	1568:1598	an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy	1568:1659	It can be implied that the CdHgTe@DMF may be an effective multifunction tool applying to optical bioimaging and magnetic targeted therapy.
27039355	5	82	theme	nm	1242:1243	arg1	range					1225:1229	the near-infrared wavelength range	1196:1229	the near-infrared wavelength range of 575-780 nm	1196:1243	The CdHgTe@DMF composite showed super-paramagnetic and photoluminescence property in the near-infrared wavelength range of 575-780 nm.
27039355	2	83	dep	transform	810:818	arg1	infrared					820:827	infrared	820:827	transform infrared	810:827	The luminescent property particle size, and internal structure of the composite were characterized using fluorescence spectrophotometer, ultraviolet spectrophotometer, laser particle size distribution, TEM, X-ray diffraction, and Fourier transform infrared.
27039355	3	84	from	study	847:851	arg1	performance					903:913	magnetic targeting performance	884:913	magnetic targeting performance of the multifunctional platform	884:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	84	from	study	847:851	arg1	tags					868:871	fluorescent tags	856:871	fluorescent tags effect and magnetic targeting performance of the multifunctional platform	856:945	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	3	84	from	study	847:851	arg1	effect					873:878	effect	873:878	effect	873:878	The experimental study on fluorescent tags effect and magnetic targeting performance of the multifunctional platform were performed by fluorescent confocal imaging.
27039355	1	85	theme	curative	516:523	arg1	effect					525:530	slow-release curative effect	503:530	slow-release curative effect	503:530	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
27039355	1	86	theme	drug	261:264	arg1	system					275:280	the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together	257:569	the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together	257:569	In this article, a water-soluble near-infrared quantum dots of CdHgTe were prepared and subsequently combined with the drug delivery system "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) to build a new nanostructure platform in form of CdHgTe@DMF, in which the fluorescent probe function of quantum dots and the magnetic targeting transport and slow-release curative effect of DMF were blended availably together.
24560618	0	0	theme	composite	90:98	arg1	design					100:105	central composite design	82:105	central composite design	82:105	Development of pectinate-ispagula mucilage mucoadhesive beads of metformin HCl by central composite design.
24560618	6	1	theme	goat	902:905	arg1	mucosa					918:923	goat intestinal mucosa	902:923	goat intestinal mucosa	902:923	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	1	2	theme	low	178:180	arg1	polymer-blend					232:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	0	3	theme	central	82:88	arg1	design					100:105	central composite design	82:105	central composite design	82:105	Development of pectinate-ispagula mucilage mucoadhesive beads of metformin HCl by central composite design.
24560618	1	4	theme	methoxy	182:188	arg1	polymer-blend					232:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	6	5	theme	hypoglycemic	941:952	arg1	effect					954:959	significant hypoglycemic effect	929:959	significant hypoglycemic effect in alloxan-induced diabetic rats	929:992	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	1	6	dep	design	298:303	arg1	point					336:340	single center point	322:340	single center point	322:340	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	1	6	dep	design	298:303	arg1	α=1.414					347:353	α=1.414	347:353	α=1.414	347:353	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	1	6	dep	design	298:303	arg1	type					316:319	spherical type	306:319	spherical type	306:319	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	6	7	with	effect	954:959	arg1	mucosa					918:923	goat intestinal mucosa	902:923	goat intestinal mucosa	902:923	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	4	8	theme	drug	696:699	arg1	release					701:707	controlled in vitro sustained drug release	666:707	controlled in vitro sustained drug release	666:707	All these beads exhibited suitable controlled in vitro sustained drug release pattern with super case-II transport mechanism over 10h.
24560618	3	9	theme	optimized	529:537	arg1	beads					539:543	The optimized beads	525:543	The optimized beads containing metformin HCl (F-O)	525:574	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	6	10	theme	significant	929:939	arg1	effect					954:959	significant hypoglycemic effect	929:959	significant hypoglycemic effect in alloxan-induced diabetic rats	929:992	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	6	11	theme	metformin	1122:1130	arg1	HCl					1132:1134	metformin HCl	1122:1134	metformin HCl maintaining tight blood glucose level and advanced patient compliance	1122:1204	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	6	12	theme	optimized	823:831	arg1	beads					833:837	The optimized beads	819:837	The optimized beads	819:837	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	6	13	theme	tight	1148:1152	arg1	level					1168:1172	tight blood glucose level	1148:1172	tight blood glucose level	1148:1172	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	4	14	theme	sustained	686:694	arg1	release					701:707	controlled in vitro sustained drug release	666:707	controlled in vitro sustained drug release	666:707	All these beads exhibited suitable controlled in vitro sustained drug release pattern with super case-II transport mechanism over 10h.
24560618	1	15	theme	central	280:286	arg1	design					298:303	central composite design	280:303	central composite design (spherical type, single center point, and α=1.414)	280:354	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	4	16	dep	suitable	657:664	arg1	release					701:707	controlled in vitro sustained drug release	666:707	controlled in vitro sustained drug release	666:707	All these beads exhibited suitable controlled in vitro sustained drug release pattern with super case-II transport mechanism over 10h.
24560618	2	17	theme	drug	397:400	arg1	DEE					428:430	DEE	428:430	DEE	428:430	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	2	17	theme	drug	397:400	arg1	efficiency					416:425	drug encapsulation efficiency	397:425	drug encapsulation efficiency (DEE)	397:431	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	2	18	theme	IHM	382:384	arg1	amounts					386:392	LM pectin and IHM amounts	368:392	amounts	386:392	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	6	19	with	swelling	867:874	arg1	mucosa					918:923	goat intestinal mucosa	902:923	goat intestinal mucosa	902:923	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	6	20	theme	HCl	1132:1134	arg1	absorption					1108:1117	prolonged systemic absorption	1089:1117	prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance	1089:1204	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	1	21	theme	LM	191:192	arg1	polymer-blend					232:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	2	22	theme	drug	448:451	arg1	release					453:459	cumulative drug release	437:459	cumulative drug release at 10h (R10h)	437:473	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	6	23	from	effect	954:959	arg1	rats					989:992	alloxan-induced diabetic rats	964:992	alloxan-induced diabetic rats	964:992	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	4	24	theme	controlled	666:675	arg1	release					701:707	controlled in vitro sustained drug release	666:707	controlled in vitro sustained drug release	666:707	All these beads exhibited suitable controlled in vitro sustained drug release pattern with super case-II transport mechanism over 10h.
24560618	6	25	from	swelling	867:874	arg1	rats					989:992	alloxan-induced diabetic rats	964:992	alloxan-induced diabetic rats	964:992	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	2	26	theme	pectin	371:376	arg1	Effects					357:363	Effects	357:363	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h)	357:473	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	1	27	theme	composite	288:296	arg1	design					298:303	central composite design	280:303	central composite design (spherical type, single center point, and α=1.414)	280:354	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	3	28	theme	R10h	608:611	arg1	DEE					583:585	DEE	583:585	DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%	583:628	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	4	29	theme	suitable	657:664	arg1	pattern					709:715	suitable controlled in vitro sustained drug release pattern	657:715	suitable controlled in vitro sustained drug release pattern	657:715	All these beads exhibited suitable controlled in vitro sustained drug release pattern with super case-II transport mechanism over 10h.
24560618	1	30	theme	pectin-ispaghula	195:210	arg1	polymer-blend					232:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	0	31	theme	mucilage	34:41	arg1	Development					0:10	Development	0:10	Development of pectinate-ispagula mucilage	0:41	Development of pectinate-ispagula mucilage mucoadhesive beads of metformin HCl by central composite design.
24560618	6	32	theme	intestinal	907:916	arg1	mucosa					918:923	goat intestinal mucosa	902:923	goat intestinal mucosa	902:923	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	2	33	from	Effects	357:363	arg1	DEE					428:430	DEE	428:430	DEE	428:430	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	2	33	from	Effects	357:363	arg1	efficiency					416:425	drug encapsulation efficiency	397:425	drug encapsulation efficiency (DEE)	397:431	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	2	33	from	Effects	357:363	arg1	release					453:459	cumulative drug release	437:459	cumulative drug release at 10h (R10h)	437:473	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	1	34	theme	Ionotropically-gelled	108:128	arg1	beads					143:147	Ionotropically-gelled mucoadhesive beads	108:147	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	108:244	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	1	35	theme	husk	212:215	arg1	polymer-blend					232:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	0	36	theme	pectinate-ispagula	15:32	arg1	mucilage					34:41	pectinate-ispagula mucilage	15:41	pectinate-ispagula mucilage	15:41	Development of pectinate-ispagula mucilage mucoadhesive beads of metformin HCl by central composite design.
24560618	3	37	contain	containing	545:554	arg2	F-O					571:573	F-O	571:573	F-O	571:573	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	3	37	contain	containing	545:554	arg1	beads					539:543	The optimized beads	525:543	The optimized beads containing metformin HCl (F-O)	525:574	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	3	37	contain	containing	545:554	arg2	HCl					566:568	metformin HCl	556:568	metformin HCl (F-O)	556:574	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	6	38	with	mucoadhesivity	882:895	arg1	mucosa					918:923	goat intestinal mucosa	902:923	goat intestinal mucosa	902:923	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	2	39	from	10h	464:466	arg1	DEE					428:430	DEE	428:430	DEE	428:430	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	2	39	from	10h	464:466	arg1	efficiency					416:425	drug encapsulation efficiency	397:425	drug encapsulation efficiency (DEE)	397:431	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	2	39	from	10h	464:466	arg1	release					453:459	cumulative drug release	437:459	cumulative drug release at 10h (R10h)	437:473	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	1	40	theme	mucoadhesive	130:141	arg1	beads					143:147	Ionotropically-gelled mucoadhesive beads	108:147	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	108:244	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	2	41	theme	cumulative	437:446	arg1	release					453:459	cumulative drug release	437:459	cumulative drug release at 10h (R10h)	437:473	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	1	42	theme	mucilage	217:224	arg1	polymer-blend					232:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	1	43	theme	spherical	306:314	arg1	type					316:319	spherical type	306:319	spherical type	306:319	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	2	44	theme	surface	504:510	arg1	methodology					512:522	response surface methodology	495:522	response surface methodology	495:522	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	3	45	theme	%	602:602	arg1	DEE					583:585	DEE	583:585	DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%	583:628	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	2	46	theme	LM	368:369	arg1	pectin					371:376	LM pectin and IHM amounts	368:392	pectin	371:376	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	6	47	theme	prolonged	999:1007	arg1	period					1009:1014	prolonged period	999:1014	prolonged period	999:1014	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	0	48	theme	mucoadhesive	43:54	arg1	beads					56:60	mucoadhesive beads	43:60	mucoadhesive beads of metformin HCl	43:77	Development of pectinate-ispagula mucilage mucoadhesive beads of metformin HCl by central composite design.
24560618	2	49	theme	response	495:502	arg1	methodology					512:522	response surface methodology	495:522	response surface methodology	495:522	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	3	50	theme	86.98	590:594	arg1	%					628:628	47.20 ± 1.28%	616:628	47.20 ± 1.28%	616:628	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	3	50	theme	86.98	590:594	arg1	%					602:602	86.98 ± 3.26%	590:602	86.98 ± 3.26%	590:602	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	6	51	from	terms	1080:1084	arg1	lucrative					1067:1075	lucrative	1067:1075	lucrative	1067:1075	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	1	52	theme	IHM	227:229	arg1	polymer-blend					232:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	178:244	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	0	53	theme	metformin	65:73	arg1	HCl					75:77	metformin HCl	65:77	metformin HCl	65:77	Development of pectinate-ispagula mucilage mucoadhesive beads of metformin HCl by central composite design.
24560618	6	54	theme	good	877:880	arg1	mucoadhesivity					882:895	good mucoadhesivity	877:895	good mucoadhesivity with goat intestinal mucosa	877:923	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	3	55	theme	±	596:596	arg1	%					628:628	47.20 ± 1.28%	616:628	47.20 ± 1.28%	616:628	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	3	55	theme	±	596:596	arg1	%					602:602	86.98 ± 3.26%	590:602	86.98 ± 3.26%	590:602	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	3	56	theme	metformin	556:564	arg1	F-O					571:573	F-O	571:573	F-O	571:573	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	3	56	theme	metformin	556:564	arg1	HCl					566:568	metformin HCl	556:568	metformin HCl (F-O)	556:574	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	6	57	theme	prolonged	1089:1097	arg1	absorption					1108:1117	prolonged systemic absorption	1089:1117	prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance	1089:1204	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	3	58	theme	1.28	624:627	arg1	±					622:622	±	622:622	±	622:622	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	1	59	theme	single	322:327	arg1	point					336:340	single center point	322:340	single center point	322:340	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	6	60	theme	pH-dependent	854:865	arg1	swelling					867:874	pH-dependent swelling	854:874	pH-dependent swelling	854:874	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	6	61	theme	patient	1187:1193	arg1	compliance					1195:1204	advanced patient compliance	1178:1204	advanced patient compliance	1178:1204	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	4	62	dep	in	677:678	arg1	vitro					680:684	vitro	680:684	vitro	680:684	All these beads exhibited suitable controlled in vitro sustained drug release pattern with super case-II transport mechanism over 10h.
24560618	1	63	theme	center	329:334	arg1	point					336:340	single center point	322:340	single center point	322:340	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	6	64	from	mucoadhesivity	882:895	arg1	rats					989:992	alloxan-induced diabetic rats	964:992	alloxan-induced diabetic rats	964:992	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	3	65	theme	47.20	616:620	arg1	±					622:622	±	622:622	±	622:622	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	4	66	theme	super	722:726	arg1	mechanism					746:754	super case-II transport mechanism	722:754	super case-II transport mechanism	722:754	All these beads exhibited suitable controlled in vitro sustained drug release pattern with super case-II transport mechanism over 10h.
24560618	6	67	theme	diabetic	980:987	arg1	rats					989:992	alloxan-induced diabetic rats	964:992	alloxan-induced diabetic rats	964:992	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	1	68	theme	metformin	152:160	arg1	HCl					162:164	metformin HCl	152:164	metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	152:244	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	6	69	theme	absorption	1108:1117	arg1	terms					1080:1084	terms	1080:1084	terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance	1080:1204	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	6	70	theme	systemic	1099:1106	arg1	absorption					1108:1117	prolonged systemic absorption	1089:1117	prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance	1089:1204	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	6	71	theme	blood	1154:1158	arg1	level					1168:1172	tight blood glucose level	1148:1172	tight blood glucose level	1148:1172	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	4	72	theme	in	677:678	arg1	release					701:707	controlled in vitro sustained drug release	666:707	controlled in vitro sustained drug release	666:707	All these beads exhibited suitable controlled in vitro sustained drug release pattern with super case-II transport mechanism over 10h.
24560618	1	73	theme	HCl	162:164	arg1	beads					143:147	Ionotropically-gelled mucoadhesive beads	108:147	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend	108:244	Ionotropically-gelled mucoadhesive beads of metformin HCl composed of low methoxy (LM) pectin-ispaghula husk mucilage (IHM) polymer-blend was developed and optimized using central composite design (spherical type, single center point, and α=1.414).
24560618	6	74	theme	glucose	1160:1166	arg1	level					1168:1172	tight blood glucose level	1148:1172	tight blood glucose level	1148:1172	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	2	75	theme	amounts	386:392	arg1	Effects					357:363	Effects	357:363	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h)	357:473	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	4	76	theme	transport	736:744	arg1	mechanism					746:754	super case-II transport mechanism	722:754	super case-II transport mechanism	722:754	All these beads exhibited suitable controlled in vitro sustained drug release pattern with super case-II transport mechanism over 10h.
24560618	2	77	theme	encapsulation	402:414	arg1	DEE					428:430	DEE	428:430	DEE	428:430	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	2	77	theme	encapsulation	402:414	arg1	efficiency					416:425	drug encapsulation efficiency	397:425	drug encapsulation efficiency (DEE)	397:431	Effects of LM pectin and IHM amounts on drug encapsulation efficiency (DEE) and cumulative drug release at 10h (R10h) were analysed using response surface methodology.
24560618	6	78	theme	oral	1022:1025	arg1	administration					1027:1040	oral administration	1022:1040	oral administration	1022:1040	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	6	79	theme	alloxan-induced	964:978	arg1	rats					989:992	alloxan-induced diabetic rats	964:992	alloxan-induced diabetic rats	964:992	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	0	80	theme	HCl	75:77	arg1	beads					56:60	mucoadhesive beads	43:60	mucoadhesive beads of metformin HCl	43:77	Development of pectinate-ispagula mucilage mucoadhesive beads of metformin HCl by central composite design.
24560618	3	81	theme	%	628:628	arg1	%					628:628	47.20 ± 1.28%	616:628	47.20 ± 1.28%	616:628	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	3	81	theme	%	628:628	arg1	R10h					608:611	R10h	608:611	R10h of 47.20 ± 1.28%	608:628	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	3	81	theme	%	628:628	arg1	%					602:602	86.98 ± 3.26%	590:602	86.98 ± 3.26%	590:602	The optimized beads containing metformin HCl (F-O) showed DEE of 86.98 ± 3.26% and R10h of 47.20 ± 1.28%.
24560618	4	82	theme	case-II	728:734	arg1	mechanism					746:754	super case-II transport mechanism	722:754	super case-II transport mechanism	722:754	All these beads exhibited suitable controlled in vitro sustained drug release pattern with super case-II transport mechanism over 10h.
24560618	6	83	from	lucrative	1067:1075	arg1	terms					1080:1084	terms	1080:1084	terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance	1080:1204	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24560618	6	84	theme	advanced	1178:1185	arg1	compliance					1195:1204	advanced patient compliance	1178:1204	advanced patient compliance	1178:1204	The optimized beads also exhibited pH-dependent swelling, good mucoadhesivity with goat intestinal mucosa and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration, which could possibly be lucrative in terms of prolonged systemic absorption of metformin HCl maintaining tight blood glucose level and advanced patient compliance.
24431079	0	0	theme	qualitative/quantitative	81:104	arg1	relationship					125:136	their qualitative/quantitative structure-activity relationship	75:136	their qualitative/quantitative structure-activity relationship	75:136	Dietary flavonoids modulate CYP2C to improve drug oral bioavailability and their qualitative/quantitative structure-activity relationship.
24431079	1	1	dep	relationships	336:348	arg1	SAR					351:353	SAR	351:353	SAR	351:353	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	7	2	theme	concentration-time	1055:1072	arg1	curve					1074:1078	the concentration-time curve	1051:1078	the concentration-time curve	1051:1078	This flavonoid enhanced the oral bioavailability of fluvastatin in vivo, producing a >2-fold increase in the area under the concentration-time curve and in the peak plasma concentration.
24431079	1	3	theme	oral	175:178	arg1	bioavailability					180:194	the drug oral bioavailability	166:194	the drug oral bioavailability	166:194	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	1	4	theme	qualitative	281:291	arg1	QSAR					311:314	QSAR	311:314	QSAR	311:314	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	1	4	theme	qualitative	281:291	arg1	relationships					336:348	qualitative and quantitative (QSAR) structure-activity relationships	281:348	qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C	281:383	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	8	5	theme	2,3-double	1164:1173	arg1	bond					1175:1178	a 2,3-double bond	1162:1178	a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation	1162:1251	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	6	from	hydroxylation	1195:1207	arg1	presence					1150:1157	the presence	1146:1157	the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation	1146:1251	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	6	7	theme	CYP2C	862:866	arg1	tamarixetin					902:912	tamarixetin	902:912	tamarixetin (IC50 = 1.4 μM)	902:928	The most potent CYP2C inhibitor identified in vitro was tamarixetin (IC50 = 1.4 μM).
24431079	6	7	theme	CYP2C	862:866	arg1	inhibitor					868:876	The most potent CYP2C inhibitor	846:876	The most potent CYP2C inhibitor identified in vitro	846:896	The most potent CYP2C inhibitor identified in vitro was tamarixetin (IC50 = 1.4 μM).
24431079	8	8	contain	had	1253:1255	arg2	effects					1267:1273	important effects	1257:1273	important effects	1257:1273	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	8	contain	had	1253:1255	arg1	presence					1150:1157	the presence	1146:1157	the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation	1146:1251	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	5	9	theme	cholesterol-reducing	766:785	arg1	fluvastatin					793:803	fluvastatin	793:803	fluvastatin	793:803	The most effective flavonoid was orally co-administered in vivo with a cholesterol-reducing drug, fluvastatin, which is normally metabolized by CYP2C.
24431079	5	9	theme	cholesterol-reducing	766:785	arg1	drug					787:790	a cholesterol-reducing drug	764:790	a cholesterol-reducing drug	764:790	The most effective flavonoid was orally co-administered in vivo with a cholesterol-reducing drug, fluvastatin, which is normally metabolized by CYP2C.
24431079	11	10	theme	therapeutic	1577:1587	arg1	efficacy					1589:1596	the therapeutic efficacy	1573:1596	the therapeutic efficacy of drugs with low bioavailability	1573:1630	Thus, the use of tamarixetin could improve the therapeutic efficacy of drugs with low bioavailability.
24431079	6	11	theme	potent	855:860	arg1	tamarixetin					902:912	tamarixetin	902:912	tamarixetin (IC50 = 1.4 μM)	902:928	The most potent CYP2C inhibitor identified in vitro was tamarixetin (IC50 = 1.4 μM).
24431079	6	11	theme	potent	855:860	arg1	inhibitor					868:876	The most potent CYP2C inhibitor	846:876	The most potent CYP2C inhibitor identified in vitro	846:896	The most potent CYP2C inhibitor identified in vitro was tamarixetin (IC50 = 1.4 μM).
24431079	8	12	from	glycosylation	1239:1251	arg1	presence					1150:1157	the presence	1146:1157	the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation	1146:1251	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	1	13	theme	quantitative	297:308	arg1	QSAR					311:314	QSAR	311:314	QSAR	311:314	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	1	13	theme	quantitative	297:308	arg1	relationships					336:348	qualitative and quantitative (QSAR) structure-activity relationships	281:348	qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C	281:383	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	0	14	theme	structure-activity	106:123	arg1	relationship					125:136	their qualitative/quantitative structure-activity relationship	75:136	their qualitative/quantitative structure-activity relationship	75:136	Dietary flavonoids modulate CYP2C to improve drug oral bioavailability and their qualitative/quantitative structure-activity relationship.
24431079	5	15	theme	effective	704:712	arg1	flavonoid					714:722	The most effective flavonoid	695:722	The most effective flavonoid	695:722	The most effective flavonoid was orally co-administered in vivo with a cholesterol-reducing drug, fluvastatin, which is normally metabolized by CYP2C.
24431079	7	16	theme	fluvastatin	983:993	arg1	bioavailability					964:978	the oral bioavailability	955:978	the oral bioavailability of fluvastatin	955:993	This flavonoid enhanced the oral bioavailability of fluvastatin in vivo, producing a >2-fold increase in the area under the concentration-time curve and in the peak plasma concentration.
24431079	4	17	theme	flavonoid	635:643	arg1	inhibition					607:616	The percent inhibition	595:616	The percent inhibition	595:616	The percent inhibition and IC50 of each flavonoid were calculated and used to develop SAR and QSAR.
24431079	4	17	theme	flavonoid	635:643	arg1	IC50					622:625	IC50	622:625	IC50 of each flavonoid	622:643	The percent inhibition and IC50 of each flavonoid were calculated and used to develop SAR and QSAR.
24431079	3	18	theme	liver	577:581	arg1	microsomes					583:592	rat liver microsomes	573:592	rat liver microsomes	573:592	Enzyme activity was determined by measuring conversion of tolbutamide to 4-hydroxytolbutamide by rat liver microsomes.
24431079	8	19	theme	C	1187:1187	arg1	ring					1189:1192	the C ring	1183:1192	the C ring	1183:1192	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	20	from	presence	1150:1157	arg1	hydroxylation					1195:1207	hydroxylation	1195:1207	hydroxylation at positions 5, 6, and 7	1195:1232	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	20	from	presence	1150:1157	arg1	glycosylation					1239:1251	glycosylation	1239:1251	glycosylation	1239:1251	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	20	from	presence	1150:1157	arg1	ring					1189:1192	the C ring	1183:1192	the C ring	1183:1192	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	9	21	theme	other	1394:1398	arg1	compounds					1424:1432	other untested flavonoid-like compounds	1394:1432	other untested flavonoid-like compounds	1394:1432	These findings should prove useful for predicting the inhibition of CYP2C activity by other untested flavonoid-like compounds.
24431079	2	22	theme	flavonoids	420:429	arg1	total					388:392	A total	386:392	A total of 40 naturally occurring flavonoids	386:429	A total of 40 naturally occurring flavonoids were screened in vitro for CYP2C inhibition.
24431079	11	23	with	efficacy	1589:1596	arg1	bioavailability					1616:1630	low bioavailability	1612:1630	low bioavailability	1612:1630	Thus, the use of tamarixetin could improve the therapeutic efficacy of drugs with low bioavailability.
24431079	6	24	theme	=	920:920	arg1	IC50					915:918	IC50	915:918	IC50 = 1.4 μM	915:927	The most potent CYP2C inhibitor identified in vitro was tamarixetin (IC50 = 1.4 μM).
24431079	10	25	theme	present	1442:1448	arg1	study					1450:1454	the present study	1438:1454	the present study	1438:1454	In the present study, tamarixetin significantly inhibited CYP2C activity in vitro and in vivo.
24431079	2	26	theme	occurring	410:418	arg1	flavonoids					420:429	40 naturally occurring flavonoids	397:429	40 naturally occurring flavonoids	397:429	A total of 40 naturally occurring flavonoids were screened in vitro for CYP2C inhibition.
24431079	0	27	theme	Dietary	0:6	arg1	flavonoids					8:17	Dietary flavonoids	0:17	Dietary flavonoids	0:17	Dietary flavonoids modulate CYP2C to improve drug oral bioavailability and their qualitative/quantitative structure-activity relationship.
24431079	8	28	from	ring	1189:1192	arg1	presence					1150:1157	the presence	1146:1157	the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation	1146:1251	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	29	theme	bond	1175:1178	arg1	presence					1150:1157	the presence	1146:1157	the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation	1146:1251	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	1	30	theme	flavonoid	222:230	arg1	inhibitors					232:241	flavonoid inhibitors	222:241	flavonoid inhibitors of the CYP2C isozyme	222:262	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	4	31	theme	percent	599:605	arg1	inhibition					607:616	The percent inhibition	595:616	The percent inhibition	595:616	The percent inhibition and IC50 of each flavonoid were calculated and used to develop SAR and QSAR.
24431079	4	32	used	used	665:668	arg2	inhibition					607:616	The percent inhibition	595:616	The percent inhibition	595:616	The percent inhibition and IC50 of each flavonoid were calculated and used to develop SAR and QSAR.
24431079	7	33	theme	oral	959:962	arg1	bioavailability					964:978	the oral bioavailability	955:978	the oral bioavailability of fluvastatin	955:993	This flavonoid enhanced the oral bioavailability of fluvastatin in vivo, producing a >2-fold increase in the area under the concentration-time curve and in the peak plasma concentration.
24431079	2	34	theme	CYP2C	458:462	arg1	inhibition					464:473	CYP2C inhibition	458:473	CYP2C inhibition	458:473	A total of 40 naturally occurring flavonoids were screened in vitro for CYP2C inhibition.
24431079	1	35	theme	structure-activity	317:334	arg1	QSAR					311:314	QSAR	311:314	QSAR	311:314	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	1	35	theme	structure-activity	317:334	arg1	relationships					336:348	qualitative and quantitative (QSAR) structure-activity relationships	281:348	qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C	281:383	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	7	36	theme	plasma	1096:1101	arg1	concentration					1103:1115	the peak plasma concentration	1087:1115	the peak plasma concentration	1087:1115	This flavonoid enhanced the oral bioavailability of fluvastatin in vivo, producing a >2-fold increase in the area under the concentration-time curve and in the peak plasma concentration.
24431079	8	37	from	bond	1175:1178	arg1	positions					1212:1220	positions 5, 6, and 7	1212:1232	positions 5, 6, and 7	1212:1232	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	37	from	bond	1175:1178	arg1	hydroxylation					1195:1207	hydroxylation	1195:1207	hydroxylation at positions 5, 6, and 7	1195:1232	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	37	from	bond	1175:1178	arg1	glycosylation					1239:1251	glycosylation	1239:1251	glycosylation	1239:1251	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	37	from	bond	1175:1178	arg1	ring					1189:1192	the C ring	1183:1192	the C ring	1183:1192	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	7	38	from	increase	1024:1031	arg1	concentration					1103:1115	the peak plasma concentration	1087:1115	the peak plasma concentration	1087:1115	This flavonoid enhanced the oral bioavailability of fluvastatin in vivo, producing a >2-fold increase in the area under the concentration-time curve and in the peak plasma concentration.
24431079	7	38	from	increase	1024:1031	arg1	area					1040:1043	the area	1036:1043	the area under the concentration-time curve	1036:1078	This flavonoid enhanced the oral bioavailability of fluvastatin in vivo, producing a >2-fold increase in the area under the concentration-time curve and in the peak plasma concentration.
24431079	6	39	dep	tamarixetin	902:912	arg1	IC50					915:918	IC50	915:918	IC50 = 1.4 μM	915:927	The most potent CYP2C inhibitor identified in vitro was tamarixetin (IC50 = 1.4 μM).
24431079	8	40	theme	SAR	1118:1120	arg1	analysis					1122:1129	SAR analysis	1118:1129	SAR analysis	1118:1129	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	11	41	theme	drugs	1601:1605	arg1	efficacy					1589:1596	the therapeutic efficacy	1573:1596	the therapeutic efficacy of drugs with low bioavailability	1573:1630	Thus, the use of tamarixetin could improve the therapeutic efficacy of drugs with low bioavailability.
24431079	1	42	theme	CYP2C	250:254	arg1	isozyme					256:262	the CYP2C isozyme	246:262	the CYP2C isozyme	246:262	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	0	43	theme	oral	50:53	arg1	bioavailability					55:69	drug oral bioavailability	45:69	drug oral bioavailability	45:69	Dietary flavonoids modulate CYP2C to improve drug oral bioavailability and their qualitative/quantitative structure-activity relationship.
24431079	8	44	theme	flavonoid-CYP2C	1278:1292	arg1	interactions					1294:1305	flavonoid-CYP2C interactions	1278:1305	flavonoid-CYP2C interactions	1278:1305	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	0	45	theme	drug	45:48	arg1	bioavailability					55:69	drug oral bioavailability	45:69	drug oral bioavailability	45:69	Dietary flavonoids modulate CYP2C to improve drug oral bioavailability and their qualitative/quantitative structure-activity relationship.
24431079	9	46	theme	CYP2C	1376:1380	arg1	activity					1382:1389	CYP2C activity	1376:1389	CYP2C activity	1376:1389	These findings should prove useful for predicting the inhibition of CYP2C activity by other untested flavonoid-like compounds.
24431079	7	47	theme	peak	1091:1094	arg1	concentration					1103:1115	the peak plasma concentration	1087:1115	the peak plasma concentration	1087:1115	This flavonoid enhanced the oral bioavailability of fluvastatin in vivo, producing a >2-fold increase in the area under the concentration-time curve and in the peak plasma concentration.
24431079	8	48	gly	glycosylation	1239:1251	arg1	positions					1212:1220	positions 5, 6, and 7	1212:1232	positions 5, 6, and 7	1212:1232	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	48	gly	glycosylation	1239:1251	arg2	positions					1212:1220	positions 5, 6, and 7	1212:1232	positions 5, 6, and 7	1212:1232	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	10	49	theme	CYP2C	1493:1497	arg1	activity					1499:1506	CYP2C activity	1493:1506	CYP2C activity	1493:1506	In the present study, tamarixetin significantly inhibited CYP2C activity in vitro and in vivo.
24431079	3	50	theme	tolbutamide	534:544	arg1	conversion					520:529	conversion	520:529	conversion of tolbutamide to 4-hydroxytolbutamide	520:568	Enzyme activity was determined by measuring conversion of tolbutamide to 4-hydroxytolbutamide by rat liver microsomes.
24431079	9	51	theme	activity	1382:1389	arg1	inhibition					1362:1371	the inhibition	1358:1371	the inhibition of CYP2C activity by other untested flavonoid-like compounds	1358:1432	These findings should prove useful for predicting the inhibition of CYP2C activity by other untested flavonoid-like compounds.
24431079	11	52	theme	tamarixetin	1547:1557	arg1	use					1540:1542	the use	1536:1542	the use of tamarixetin	1536:1557	Thus, the use of tamarixetin could improve the therapeutic efficacy of drugs with low bioavailability.
24431079	1	53	theme	isozyme	256:262	arg1	inhibitors					232:241	flavonoid inhibitors	222:241	flavonoid inhibitors of the CYP2C isozyme	222:262	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	1	54	with	co-administration	199:215	arg1	inhibitors					232:241	flavonoid inhibitors	222:241	flavonoid inhibitors of the CYP2C isozyme	222:262	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	7	55	theme	>2-fold	1016:1022	arg1	increase					1024:1031	a >2-fold increase	1014:1031	a >2-fold increase in the area under the concentration-time curve and in the peak plasma concentration	1014:1115	This flavonoid enhanced the oral bioavailability of fluvastatin in vivo, producing a >2-fold increase in the area under the concentration-time curve and in the peak plasma concentration.
24431079	1	56	theme	drug	170:173	arg1	bioavailability					180:194	the drug oral bioavailability	166:194	the drug oral bioavailability	166:194	This study aims to improve the drug oral bioavailability by co-administration with flavonoid inhibitors of the CYP2C isozyme and to establish qualitative and quantitative (QSAR) structure-activity relationships (SAR) between flavonoids and CYP2C.
24431079	6	57	dep	=	920:920	arg1	μM					926:927	1.4 μM	922:927	1.4 μM	922:927	The most potent CYP2C inhibitor identified in vitro was tamarixetin (IC50 = 1.4 μM).
24431079	8	58	theme	important	1257:1265	arg1	effects					1267:1273	important effects	1257:1273	important effects	1257:1273	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	3	59	theme	Enzyme	476:481	arg1	activity					483:490	Enzyme activity	476:490	Enzyme activity	476:490	Enzyme activity was determined by measuring conversion of tolbutamide to 4-hydroxytolbutamide by rat liver microsomes.
24431079	9	60	theme	untested	1400:1407	arg1	compounds					1424:1432	other untested flavonoid-like compounds	1394:1432	other untested flavonoid-like compounds	1394:1432	These findings should prove useful for predicting the inhibition of CYP2C activity by other untested flavonoid-like compounds.
24431079	8	61	from	positions	1212:1220	arg1	bond					1175:1178	a 2,3-double bond	1162:1178	a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation	1162:1251	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	61	from	positions	1212:1220	arg1	hydroxylation					1195:1207	hydroxylation	1195:1207	hydroxylation at positions 5, 6, and 7	1195:1232	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	61	from	positions	1212:1220	arg1	glycosylation					1239:1251	glycosylation	1239:1251	glycosylation	1239:1251	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	8	61	from	positions	1212:1220	arg1	ring					1189:1192	the C ring	1183:1192	the C ring	1183:1192	SAR analysis indicated that the presence of a 2,3-double bond in the C ring, hydroxylation at positions 5, 6, and 7, and glycosylation had important effects on flavonoid-CYP2C interactions.
24431079	11	62	theme	low	1612:1614	arg1	bioavailability					1616:1630	low bioavailability	1612:1630	low bioavailability	1612:1630	Thus, the use of tamarixetin could improve the therapeutic efficacy of drugs with low bioavailability.
24431079	3	63	theme	rat	573:575	arg1	microsomes					583:592	rat liver microsomes	573:592	rat liver microsomes	573:592	Enzyme activity was determined by measuring conversion of tolbutamide to 4-hydroxytolbutamide by rat liver microsomes.
24431079	9	64	theme	flavonoid-like	1409:1422	arg1	compounds					1424:1432	other untested flavonoid-like compounds	1394:1432	other untested flavonoid-like compounds	1394:1432	These findings should prove useful for predicting the inhibition of CYP2C activity by other untested flavonoid-like compounds.
24713107	12	0	theme	Larger	1642:1647	arg1	studies					1649:1655	Larger studies	1642:1655	Larger studies	1642:1655	Larger studies are needed to address this issue.
24713107	10	1	dep	HR	1271:1272	arg1	1.55-14.5					1286:1294	1.55-14.5	1286:1294	1.55-14.5	1286:1294	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	10	1	dep	HR	1271:1272	arg1	%					1283:1283	CI95%	1279:1283	HR 4.73 CI95%	1271:1283	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	6	2	dep	age	837:839	arg1	±					844:844	61 ± 18	841:847	61 ± 18	841:847	RESULTS 90 patients (age 61 ± 18, male 48.9%) were enrolled.
24713107	2	3	theme	term	235:238	arg1	outcome					240:246	IDDVT long term outcome	224:246	IDDVT long term outcome	224:246	AIMS To assess IDDVT long term outcome and the impact of IDDVT characteristics on outcome.
24713107	5	4	theme	recurrence/extension	768:787	arg1	recurrence/extension					768:787	IDDVT recurrence/extension	762:787	IDDVT recurrence/extension	762:787	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	4	theme	recurrence/extension	768:787	arg1	composite					683:691	the composite	679:691	the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension	679:787	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	4	theme	recurrence/extension	768:787	arg1	thrombosis					740:749	proximal deep vein thrombosis	721:749	proximal deep vein thrombosis (DVT)	721:755	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	4	theme	recurrence/extension	768:787	arg1	embolism					706:713	pulmonary embolism	696:713	pulmonary embolism (PE)	696:718	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	11	5	from	patients	1598:1605	arg1	relevant					1581:1588	relevant	1581:1588	relevant	1581:1588	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT may be relevant in male patients or in patients with active cancer.
24713107	10	6	dep	sex	1266:1268	arg1	HR					1271:1272	HR 4.73	1271:1277	HR 4.73 CI95%	1271:1283	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	2	7	theme	IDDVT	266:270	arg1	characteristics					272:286	IDDVT characteristics	266:286	IDDVT characteristics	266:286	AIMS To assess IDDVT long term outcome and the impact of IDDVT characteristics on outcome.
24713107	5	8	theme	24	796:797	arg1	month					799:803	month	799:803	month	799:803	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	4	9	theme	vitamin	595:601	arg1	VKA					618:620	VKA	618:620	VKA	618:620	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	4	9	theme	vitamin	595:601	arg1	antagonists					605:615	vitamin K antagonists	595:615	vitamin K antagonists (VKA)	595:621	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	1	10	theme	term	176:179	arg1	outcome					181:187	its long term outcome	167:187	its long term outcome	167:187	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	6	11	theme	male	850:853	arg1	%					859:859	male 48.9%	850:859	male 48.9%	850:859	RESULTS 90 patients (age 61 ± 18, male 48.9%) were enrolled.
24713107	6	11	theme	male	850:853	arg1	age					837:839	age 61 ± 18, male 48.9%	837:859	age	837:839	RESULTS 90 patients (age 61 ± 18, male 48.9%) were enrolled.
24713107	7	12	theme	previous	974:981	arg1	DVT					983:985	previous DVT	974:985	previous DVT (15.6%)	974:993	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	7	12	theme	previous	974:981	arg1	%					992:992	15.6%	988:992	15.6%	988:992	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	7	13	from	cancer	999:1004	arg1	patients					1011:1018	8 patients	1009:1018	8 patients (8.9%)	1009:1025	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	7	13	from	cancer	999:1004	arg1	%					1024:1024	8.9%	1021:1024	8.9%	1021:1024	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	10	14	theme	complications	1391:1403	arg1	risk					1383:1386	a higher risk	1374:1386	a higher risk of complications	1374:1403	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	2	15	from	outcome	240:246	arg1	outcome					291:297	outcome	291:297	outcome	291:297	AIMS To assess IDDVT long term outcome and the impact of IDDVT characteristics on outcome.
24713107	2	16	theme	long	230:233	arg1	outcome					240:246	IDDVT long term outcome	224:246	IDDVT long term outcome	224:246	AIMS To assess IDDVT long term outcome and the impact of IDDVT characteristics on outcome.
24713107	2	17	theme	IDDVT	224:228	arg1	outcome					240:246	IDDVT long term outcome	224:246	IDDVT long term outcome	224:246	AIMS To assess IDDVT long term outcome and the impact of IDDVT characteristics on outcome.
24713107	11	18	theme	venous	1539:1544	arg1	thromboembolism					1546:1560	recurrent venous thromboembolism	1529:1560	recurrent venous thromboembolism	1529:1560	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT may be relevant in male patients or in patients with active cancer.
24713107	5	19	theme	pulmonary	696:704	arg1	PE					716:717	PE	716:717	PE	716:717	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	19	theme	pulmonary	696:704	arg1	embolism					706:713	pulmonary embolism	696:713	pulmonary embolism (PE)	696:718	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	10	20	theme	anatomical	1420:1429	arg1	characteristics					1431:1445	IDDVT anatomical characteristics	1414:1445	IDDVT anatomical characteristics	1414:1445	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	9	21	theme	proximal	1209:1216	arg1	DVTs					1218:1221	4 proximal DVTs	1207:1221	4 proximal DVTs (one in cancer patient)	1207:1245	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	1	22	theme	symptomatic	138:148	arg1	outpatients					150:160	symptomatic outpatients	138:160	symptomatic outpatients	138:160	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	3	23	theme	prospective	313:323	arg1	study					340:344	a prospective, single center study	311:344	a prospective, single center study	311:344	METHODS In a prospective, single center study we enrolled symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography.
24713107	1	24	located	found	129:133	arg2	thrombosis					96:105	BACKGROUND Isolated distal deep vein thrombosis	59:105	BACKGROUND Isolated distal deep vein thrombosis (IDDVT)	59:113	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	1	24	located	found	129:133	arg1	outpatients					150:160	symptomatic outpatients	138:160	symptomatic outpatients	138:160	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	1	24	located	found	129:133	arg2	IDDVT					108:112	IDDVT	108:112	IDDVT	108:112	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	7	25	from	DVT	983:985	arg1	patients					1011:1018	8 patients	1009:1018	8 patients (8.9%)	1009:1025	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	7	25	from	DVT	983:985	arg1	%					1024:1024	8.9%	1021:1024	8.9%	1021:1024	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	5	26	theme	composite	683:691	arg1	end-point					653:661	The primary end-point	641:661	The primary end-point	641:661	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	26	theme	composite	683:691	arg1	rate					671:674	the rate	667:674	the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension	667:787	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	3	27	located	detected	400:407	arg1	outpatients					370:380	symptomatic outpatients	358:380	symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography	358:448	METHODS In a prospective, single center study we enrolled symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography.
24713107	3	27	located	detected	400:407	arg2	IDDVT					390:394	IDDVT	390:394	IDDVT	390:394	METHODS In a prospective, single center study we enrolled symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography.
24713107	1	28	theme	Isolated	70:77	arg1	IDDVT					108:112	IDDVT	108:112	IDDVT	108:112	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	1	28	theme	Isolated	70:77	arg1	thrombosis					96:105	BACKGROUND Isolated distal deep vein thrombosis	59:105	BACKGROUND Isolated distal deep vein thrombosis (IDDVT)	59:113	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	9	29	dep	DVTs	1218:1221	arg1	one					1224:1226	one	1224:1226	one	1224:1226	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	5	30	theme	vein	735:738	arg1	DVT					752:754	DVT	752:754	DVT	752:754	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	30	theme	vein	735:738	arg1	thrombosis					740:749	proximal deep vein thrombosis	721:749	proximal deep vein thrombosis (DVT)	721:755	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	1	31	theme	deep	86:89	arg1	IDDVT					108:112	IDDVT	108:112	IDDVT	108:112	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	1	31	theme	deep	86:89	arg1	thrombosis					96:105	BACKGROUND Isolated distal deep vein thrombosis	59:105	BACKGROUND Isolated distal deep vein thrombosis (IDDVT)	59:113	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	3	32	theme	compression	422:432	arg1	ultrasonography					434:448	whole-leg compression ultrasonography	412:448	whole-leg compression ultrasonography	412:448	METHODS In a prospective, single center study we enrolled symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography.
24713107	0	33	theme	isolated	21:28	arg1	thrombosis					47:56	isolated distal deep vein thrombosis	21:56	isolated distal deep vein thrombosis	21:56	Two years outcome of isolated distal deep vein thrombosis.
24713107	11	34	dep	CONCLUSIONS	1505:1515	arg1	risk					1521:1524	The risk	1517:1524	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT	1505:1572	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT may be relevant in male patients or in patients with active cancer.
24713107	9	35	from	two	1182:1184	arg1	patients					1196:1203	cancer patients	1189:1203	cancer patients	1189:1203	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	4	36	theme	low	498:500	arg1	LMWH					529:532	LMWH	529:532	LMWH	529:532	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	4	36	theme	low	498:500	arg1	heparins					519:526	low molecular weight heparins	498:526	low molecular weight heparins (LMWH)	498:533	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	0	37	theme	thrombosis	47:56	arg1	outcome					10:16	outcome	10:16	outcome of isolated distal deep vein thrombosis	10:56	Two years outcome of isolated distal deep vein thrombosis.
24713107	5	38	theme	proximal	721:728	arg1	DVT					752:754	DVT	752:754	DVT	752:754	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	38	theme	proximal	721:728	arg1	thrombosis					740:749	proximal deep vein thrombosis	721:749	proximal deep vein thrombosis (DVT)	721:755	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	9	39	from	PE	1178:1179	arg1	patient					1238:1244	cancer patient	1231:1244	cancer patient	1231:1244	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	4	40	theme	weight	512:517	arg1	LMWH					529:532	LMWH	529:532	LMWH	529:532	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	4	40	theme	weight	512:517	arg1	heparins					519:526	low molecular weight heparins	498:526	low molecular weight heparins (LMWH)	498:533	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	3	41	dep	prospective	313:323	arg1	single					326:331	single	326:331	single	326:331	METHODS In a prospective, single center study we enrolled symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography.
24713107	10	42	dep	1.55-14.5	1286:1294	arg1	p=0.006					1297:1303	p=0.006	1297:1303	p=0.006	1297:1303	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	0	43	dep	outcome	10:16	arg1	years					4:8	Two years	0:8	Two years	0:8	Two years outcome of isolated distal deep vein thrombosis.
24713107	7	44	from	mobility	918:925	arg1	patients					1011:1018	8 patients	1009:1018	8 patients (8.9%)	1009:1025	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	7	44	from	mobility	918:925	arg1	%					1024:1024	8.9%	1021:1024	8.9%	1021:1024	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	10	45	theme	provoked	1480:1487	arg1	IDDVT					1489:1493	provoked IDDVT	1480:1493	provoked IDDVT	1480:1493	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	9	46	dep	PE	1178:1179	arg1	two					1182:1184	two	1182:1184	two	1182:1184	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	11	47	theme	recurrent	1529:1537	arg1	thromboembolism					1546:1560	recurrent venous thromboembolism	1529:1560	recurrent venous thromboembolism	1529:1560	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT may be relevant in male patients or in patients with active cancer.
24713107	11	48	with	patients	1613:1620	arg1	cancer					1634:1639	active cancer	1627:1639	active cancer	1627:1639	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT may be relevant in male patients or in patients with active cancer.
24713107	7	49	from	surgery	953:959	arg1	patients					1011:1018	8 patients	1009:1018	8 patients (8.9%)	1009:1025	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	7	49	from	surgery	953:959	arg1	%					1024:1024	8.9%	1021:1024	8.9%	1021:1024	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	4	50	with	Patients	451:458	arg1	IDDVT					474:478	provoked IDDVT	465:478	provoked IDDVT	465:478	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	9	51	from	DVTs	1218:1221	arg1	patient					1238:1244	cancer patient	1231:1244	cancer patient	1231:1244	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	10	52	theme	therapy	1462:1468	arg1	type					1470:1473	anticoagulant therapy type	1448:1473	anticoagulant therapy type	1448:1473	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	11	53	from	patients	1613:1620	arg1	relevant					1581:1588	relevant	1581:1588	relevant	1581:1588	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT may be relevant in male patients or in patients with active cancer.
24713107	11	54	from	relevant	1581:1588	arg1	patients					1613:1620	patients	1613:1620	patients with active cancer	1613:1639	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT may be relevant in male patients or in patients with active cancer.
24713107	11	54	from	relevant	1581:1588	arg1	patients					1598:1605	male patients	1593:1605	male patients	1593:1605	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT may be relevant in male patients or in patients with active cancer.
24713107	7	55	from	obesity	936:942	arg1	patients					1011:1018	8 patients	1009:1018	8 patients (8.9%)	1009:1025	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	7	55	from	obesity	936:942	arg1	%					1024:1024	8.9%	1021:1024	8.9%	1021:1024	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	5	56	theme	IDDVT	762:766	arg1	recurrence/extension					768:787	IDDVT recurrence/extension	762:787	IDDVT recurrence/extension	762:787	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	0	57	theme	deep	37:40	arg1	thrombosis					47:56	isolated distal deep vein thrombosis	21:56	isolated distal deep vein thrombosis	21:56	Two years outcome of isolated distal deep vein thrombosis.
24713107	2	58	theme	characteristics	272:286	arg1	outcome					240:246	IDDVT long term outcome	224:246	IDDVT long term outcome	224:246	AIMS To assess IDDVT long term outcome and the impact of IDDVT characteristics on outcome.
24713107	2	58	theme	characteristics	272:286	arg1	impact					256:261	the impact	252:261	the impact of IDDVT characteristics on outcome	252:297	AIMS To assess IDDVT long term outcome and the impact of IDDVT characteristics on outcome.
24713107	10	59	theme	Male	1261:1264	arg1	sex					1266:1268	Male sex	1261:1268	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006)	1261:1304	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	5	60	theme	primary	645:651	arg1	end-point					653:661	The primary end-point	641:661	The primary end-point	641:661	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	60	theme	primary	645:651	arg1	rate					671:674	the rate	667:674	the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension	667:787	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	10	61	theme	higher	1376:1381	arg1	risk					1383:1386	a higher risk	1374:1386	a higher risk of complications	1374:1403	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	1	62	theme	long	171:174	arg1	outcome					181:187	its long term outcome	167:187	its long term outcome	167:187	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	10	63	theme	IDDVT	1414:1418	arg1	characteristics					1431:1445	IDDVT anatomical characteristics	1414:1445	IDDVT anatomical characteristics	1414:1445	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	4	64	theme	K	603:603	arg1	VKA					618:620	VKA	618:620	VKA	618:620	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	4	64	theme	K	603:603	arg1	antagonists					605:615	vitamin K antagonists	595:615	vitamin K antagonists (VKA)	595:621	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	11	65	theme	male	1593:1596	arg1	patients					1598:1605	male patients	1593:1605	male patients	1593:1605	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT may be relevant in male patients or in patients with active cancer.
24713107	9	66	theme	cancer	1189:1194	arg1	patients					1196:1203	cancer patients	1189:1203	cancer patients	1189:1203	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	7	67	theme	reduced	910:916	arg1	mobility					918:925	reduced mobility	910:925	reduced mobility (34.4%)	910:933	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	7	67	theme	reduced	910:916	arg1	%					932:932	34.4%	928:932	34.4%	928:932	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	10	68	theme	anticoagulant	1448:1460	arg1	type					1470:1473	anticoagulant therapy type	1448:1473	anticoagulant therapy type	1448:1473	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	9	69	from	IDDVT	1254:1258	arg1	patient					1238:1244	cancer patient	1231:1244	cancer patient	1231:1244	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	3	70	from	METHODS	300:306	arg1	study					340:344	a prospective, single center study	311:344	a prospective, single center study	311:344	METHODS In a prospective, single center study we enrolled symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography.
24713107	7	71	theme	Risk	877:880	arg1	factors					882:888	Risk factors	877:888	Risk factors for thrombosis	877:903	Risk factors for thrombosis were reduced mobility (34.4%), obesity (25.3%), surgery (15.6%), and previous DVT (15.6%) and cancer in 8 patients (8.9%).
24713107	5	72	theme	month	799:803	arg1	follow-up					805:813	24 month follow-up	796:813	24 month follow-up	796:813	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	73	theme	embolism	706:713	arg1	recurrence/extension					768:787	IDDVT recurrence/extension	762:787	IDDVT recurrence/extension	762:787	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	73	theme	embolism	706:713	arg1	composite					683:691	the composite	679:691	the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension	679:787	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	73	theme	embolism	706:713	arg1	thrombosis					740:749	proximal deep vein thrombosis	721:749	proximal deep vein thrombosis (DVT)	721:755	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	73	theme	embolism	706:713	arg1	embolism					706:713	pulmonary embolism	696:713	pulmonary embolism (PE)	696:718	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	2	74	from	impact	256:261	arg1	outcome					291:297	outcome	291:297	outcome	291:297	AIMS To assess IDDVT long term outcome and the impact of IDDVT characteristics on outcome.
24713107	10	75	dep	associated	1358:1367	arg1	whereas					1406:1412	whereas	1406:1412	whereas	1406:1412	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	8	76	dep	treated	1055:1061	arg1	32					1081:1082	32	1081:1082	32	1081:1082	Eighty-eight patients were treated (56 with LMWH and 32 with VKA).
24713107	8	76	dep	treated	1055:1061	arg1	56					1064:1065	56	1064:1065	56	1064:1065	Eighty-eight patients were treated (56 with LMWH and 32 with VKA).
24713107	1	77	theme	BACKGROUND	59:68	arg1	IDDVT					108:112	IDDVT	108:112	IDDVT	108:112	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	1	77	theme	BACKGROUND	59:68	arg1	thrombosis					96:105	BACKGROUND Isolated distal deep vein thrombosis	59:105	BACKGROUND Isolated distal deep vein thrombosis (IDDVT)	59:113	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	4	78	theme	provoked	465:472	arg1	IDDVT					474:478	provoked IDDVT	465:478	provoked IDDVT	465:478	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	5	79	theme	deep	730:733	arg1	DVT					752:754	DVT	752:754	DVT	752:754	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	79	theme	deep	730:733	arg1	thrombosis					740:749	proximal deep vein thrombosis	721:749	proximal deep vein thrombosis (DVT)	721:755	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	1	80	theme	distal	79:84	arg1	IDDVT					108:112	IDDVT	108:112	IDDVT	108:112	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	1	80	theme	distal	79:84	arg1	thrombosis					96:105	BACKGROUND Isolated distal deep vein thrombosis	59:105	BACKGROUND Isolated distal deep vein thrombosis (IDDVT)	59:113	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	10	81	dep	HR	1318:1319	arg1	%					1330:1330	CI95%	1326:1330	HR 5.47 CI95%	1318:1330	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	10	81	dep	HR	1318:1319	arg1	p=0.003					1344:1350	p=0.003	1344:1350	p=0.003	1344:1350	Male sex (HR 4.73 CI95%: 1.55-14.5; p=0.006) and cancer (HR 5.47 CI95%: 1.76-17.6; p=0.003) were associated with a higher risk of complications, whereas IDDVT anatomical characteristics, anticoagulant therapy type, and provoked IDDVT were not.
24713107	11	82	theme	active	1627:1632	arg1	cancer					1634:1639	active cancer	1627:1639	active cancer	1627:1639	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT may be relevant in male patients or in patients with active cancer.
24713107	5	83	theme	thrombosis	740:749	arg1	recurrence/extension					768:787	IDDVT recurrence/extension	762:787	IDDVT recurrence/extension	762:787	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	83	theme	thrombosis	740:749	arg1	composite					683:691	the composite	679:691	the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension	679:787	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	83	theme	thrombosis	740:749	arg1	thrombosis					740:749	proximal deep vein thrombosis	721:749	proximal deep vein thrombosis (DVT)	721:755	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	5	83	theme	thrombosis	740:749	arg1	embolism					706:713	pulmonary embolism	696:713	pulmonary embolism (PE)	696:718	The primary end-point was the rate of the composite of pulmonary embolism (PE), proximal deep vein thrombosis (DVT), and IDDVT recurrence/extension during 24 month follow-up.
24713107	1	84	theme	vein	91:94	arg1	IDDVT					108:112	IDDVT	108:112	IDDVT	108:112	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	1	84	theme	vein	91:94	arg1	thrombosis					96:105	BACKGROUND Isolated distal deep vein thrombosis	59:105	BACKGROUND Isolated distal deep vein thrombosis (IDDVT)	59:113	BACKGROUND Isolated distal deep vein thrombosis (IDDVT) is frequently found in symptomatic outpatients, but its long term outcome is still uncertain.
24713107	3	85	theme	center	333:338	arg1	study					340:344	a prospective, single center study	311:344	a prospective, single center study	311:344	METHODS In a prospective, single center study we enrolled symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography.
24713107	9	86	theme	cancer	1231:1236	arg1	patient					1238:1244	cancer patient	1231:1244	cancer patient	1231:1244	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	11	87	theme	thromboembolism	1546:1560	arg1	risk					1521:1524	The risk	1517:1524	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT	1505:1572	CONCLUSIONS The risk of recurrent venous thromboembolism after IDDVT may be relevant in male patients or in patients with active cancer.
24713107	0	88	theme	distal	30:35	arg1	thrombosis					47:56	isolated distal deep vein thrombosis	21:56	isolated distal deep vein thrombosis	21:56	Two years outcome of isolated distal deep vein thrombosis.
24713107	4	89	theme	molecular	502:510	arg1	LMWH					529:532	LMWH	529:532	LMWH	529:532	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	4	89	theme	molecular	502:510	arg1	heparins					519:526	low molecular weight heparins	498:526	low molecular weight heparins (LMWH)	498:533	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	3	90	dep	METHODS	300:306	arg1	enrolled					349:356	enrolled	349:356	enrolled symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography	349:448	METHODS In a prospective, single center study we enrolled symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography.
24713107	3	91	theme	whole-leg	412:420	arg1	ultrasonography					434:448	whole-leg compression ultrasonography	412:448	whole-leg compression ultrasonography	412:448	METHODS In a prospective, single center study we enrolled symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography.
24713107	0	92	theme	vein	42:45	arg1	thrombosis					47:56	isolated distal deep vein thrombosis	21:56	isolated distal deep vein thrombosis	21:56	Two years outcome of isolated distal deep vein thrombosis.
24713107	9	93	theme	median	1113:1118	arg1	follow-up					1102:1110	follow-up	1102:1110	follow-up (median 24 ± 2 months)	1102:1133	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	9	93	theme	median	1113:1118	arg1	months					1127:1132	median 24 ± 2 months	1113:1132	median 24 ± 2 months	1113:1132	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	3	94	theme	symptomatic	358:368	arg1	outpatients					370:380	symptomatic outpatients	358:380	symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography	358:448	METHODS In a prospective, single center study we enrolled symptomatic outpatients in whom IDDVT was detected by whole-leg compression ultrasonography.
24713107	9	95	theme	±	1123:1123	arg1	follow-up					1102:1110	follow-up	1102:1110	follow-up (median 24 ± 2 months)	1102:1133	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	9	95	theme	±	1123:1123	arg1	months					1127:1132	median 24 ± 2 months	1113:1132	median 24 ± 2 months	1113:1132	During follow-up (median 24 ± 2 months), 17 events were recorded, which included 3 PE (two in cancer patients), 4 proximal DVTs (one in cancer patient) and 10 IDDVT.
24713107	4	96	theme	unprovoked	564:573	arg1	IDDVT					575:579	unprovoked IDDVT	564:579	unprovoked IDDVT	564:579	Patients with provoked IDDVT were treated with low molecular weight heparins (LMWH) for 30 days while those with unprovoked IDDVT received with vitamin K antagonists (VKA) for three months.
24713107	6	97	dep	RESULTS	816:822	arg1	patients					827:834	90 patients	824:834	RESULTS 90 patients (age 61 ± 18, male 48.9%)	816:860	RESULTS 90 patients (age 61 ± 18, male 48.9%) were enrolled.
24713107	6	97	dep	RESULTS	816:822	arg1	age					837:839	age 61 ± 18, male 48.9%	837:859	age	837:839	RESULTS 90 patients (age 61 ± 18, male 48.9%) were enrolled.
26766869	5	0	from	pg/ml	771:775	arg1	range					784:788	the range	780:788	the range of 0.25-1000 pg/ml	780:807	The method was found to be simple, reliable and capable of high-sensitive detection of human serum albumin as a model, down to 0.2 pg/ml in the range of 0.25-1000 pg/ml.
26766869	3	1	theme	Au	505:506	arg1	nanoparticles					508:520	Au nanoparticles	505:520	Au nanoparticles (NPs)	505:526	The tag was shown to be capable of amplifying the recognition signal by high-density assembly of Au nanoparticles (NPs) on TMC/Fe3O4 particles.
26766869	3	1	theme	Au	505:506	arg1	NPs					523:525	NPs	523:525	NPs	523:525	The tag was shown to be capable of amplifying the recognition signal by high-density assembly of Au nanoparticles (NPs) on TMC/Fe3O4 particles.
26766869	4	2	theme	AuNPs	583:587	arg1	conductivity					567:578	The remarkable conductivity	552:578	The remarkable conductivity of AuNPs	552:587	The remarkable conductivity of AuNPs provides a feasible pathway for electron transfer.
26766869	6	3	theme	sensitive	875:883	arg1	detection					895:903	sensitive and rapid detection	875:903	sensitive and rapid detection of various analytes	875:923	Findings of the present study would create new opportunities for sensitive and rapid detection of various analytes.
26766869	1	4	theme	novel	190:194	arg1	method					217:222	a novel signal amplification method	188:222	a novel signal amplification method for high-sensitive electrochemical immunosensing	188:271	This study presents a novel signal amplification method for high-sensitive electrochemical immunosensing.
26766869	3	5	theme	TMC/Fe3O4	531:539	arg1	particles					541:549	TMC/Fe3O4 particles	531:549	TMC/Fe3O4 particles	531:549	The tag was shown to be capable of amplifying the recognition signal by high-density assembly of Au nanoparticles (NPs) on TMC/Fe3O4 particles.
26766869	0	6	theme	tracer	111:116	arg1	tag					118:120	a tracer tag	109:120	a tracer tag for high-sensitive electrochemical detection	109:165	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	0	6	theme	tracer	111:116	arg1	nanoparticles					92:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	2	7	theme	oxide	316:320	arg1	nanocomposite					349:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	7	theme	oxide	316:320	arg1	tag					385:387	a tracing tag	375:387	a tracing tag to label antibody	375:405	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	0	8	theme	high-sensitive	126:139	arg1	detection					157:165	high-sensitive electrochemical detection	126:165	high-sensitive electrochemical detection	126:165	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	6	9	theme	new	853:855	arg1	opportunities					857:869	new opportunities	853:869	new opportunities for sensitive and rapid detection of various analytes	853:923	Findings of the present study would create new opportunities for sensitive and rapid detection of various analytes.
26766869	5	10	dep	0.2	767:769	arg1	to					764:765	to	764:765	to	764:765	The method was found to be simple, reliable and capable of high-sensitive detection of human serum albumin as a model, down to 0.2 pg/ml in the range of 0.25-1000 pg/ml.
26766869	5	11	theme	pg/ml	803:807	arg1	range					784:788	the range	780:788	the range of 0.25-1000 pg/ml	780:807	The method was found to be simple, reliable and capable of high-sensitive detection of human serum albumin as a model, down to 0.2 pg/ml in the range of 0.25-1000 pg/ml.
26766869	2	12	theme	/iron	310:314	arg1	oxide					316:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide	274:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	12	theme	/iron	310:314	arg1	Fe3O4					323:327	Fe3O4	323:327	Fe3O4	323:327	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	12	theme	/iron	310:314	arg1	shell/shell/core					331:346	shell/shell/core	331:346	shell/shell/core	331:346	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	1	13	theme	signal	196:201	arg1	method					217:222	a novel signal amplification method	188:222	a novel signal amplification method for high-sensitive electrochemical immunosensing	188:271	This study presents a novel signal amplification method for high-sensitive electrochemical immunosensing.
26766869	5	14	theme	albumin	739:745	arg1	detection					714:722	high-sensitive detection	699:722	high-sensitive detection of human serum albumin	699:745	The method was found to be simple, reliable and capable of high-sensitive detection of human serum albumin as a model, down to 0.2 pg/ml in the range of 0.25-1000 pg/ml.
26766869	1	15	theme	amplification	203:215	arg1	method					217:222	a novel signal amplification method	188:222	a novel signal amplification method for high-sensitive electrochemical immunosensing	188:271	This study presents a novel signal amplification method for high-sensitive electrochemical immunosensing.
26766869	0	16	theme	amplification	7:19	arg1	strategy					21:28	Signal amplification strategy	0:28	Signal amplification strategy	0:28	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	0	17	theme	Signal	0:5	arg1	amplification					7:19	Signal amplification	0:19	Signal amplification strategy	0:28	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	3	18	from	assembly	493:500	arg1	particles					541:549	TMC/Fe3O4 particles	531:549	TMC/Fe3O4 particles	531:549	The tag was shown to be capable of amplifying the recognition signal by high-density assembly of Au nanoparticles (NPs) on TMC/Fe3O4 particles.
26766869	2	19	used	used	367:370	arg2	tag					385:387	a tracing tag	375:387	a tracing tag to label antibody	375:405	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	19	used	used	367:370	arg2	nanocomposite					349:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	1	20	theme	high-sensitive	228:241	arg1	immunosensing					259:271	high-sensitive electrochemical immunosensing	228:271	high-sensitive electrochemical immunosensing	228:271	This study presents a novel signal amplification method for high-sensitive electrochemical immunosensing.
26766869	0	21	theme	electrochemical	141:155	arg1	detection					157:165	high-sensitive electrochemical detection	126:165	high-sensitive electrochemical detection	126:165	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	6	22	theme	study	834:838	arg1	Findings					810:817	Findings	810:817	Findings of the present study	810:838	Findings of the present study would create new opportunities for sensitive and rapid detection of various analytes.
26766869	3	23	theme	nanoparticles	508:520	arg1	assembly					493:500	high-density assembly	480:500	high-density assembly of Au nanoparticles (NPs) on TMC/Fe3O4 particles	480:549	The tag was shown to be capable of amplifying the recognition signal by high-density assembly of Au nanoparticles (NPs) on TMC/Fe3O4 particles.
26766869	1	24	theme	electrochemical	243:257	arg1	immunosensing					259:271	high-sensitive electrochemical immunosensing	228:271	high-sensitive electrochemical immunosensing	228:271	This study presents a novel signal amplification method for high-sensitive electrochemical immunosensing.
26766869	0	25	theme	chitosan/iron	53:65	arg1	tag					118:120	a tracer tag	109:120	a tracer tag for high-sensitive electrochemical detection	109:165	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	0	25	theme	chitosan/iron	53:65	arg1	nanoparticles					92:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	4	26	theme	remarkable	556:565	arg1	conductivity					567:578	The remarkable conductivity	552:578	The remarkable conductivity of AuNPs	552:587	The remarkable conductivity of AuNPs provides a feasible pathway for electron transfer.
26766869	5	27	theme	high-sensitive	699:712	arg1	detection					714:722	high-sensitive detection	699:722	high-sensitive detection of human serum albumin	699:745	The method was found to be simple, reliable and capable of high-sensitive detection of human serum albumin as a model, down to 0.2 pg/ml in the range of 0.25-1000 pg/ml.
26766869	6	28	theme	present	826:832	arg1	study					834:838	the present study	822:838	the present study	822:838	Findings of the present study would create new opportunities for sensitive and rapid detection of various analytes.
26766869	0	29	theme	gold/N-trimethyl	36:51	arg1	tag					118:120	a tracer tag	109:120	a tracer tag for high-sensitive electrochemical detection	109:165	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	0	29	theme	gold/N-trimethyl	36:51	arg1	nanoparticles					92:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	6	30	theme	analytes	916:923	arg1	detection					895:903	sensitive and rapid detection	875:903	sensitive and rapid detection of various analytes	875:923	Findings of the present study would create new opportunities for sensitive and rapid detection of various analytes.
26766869	5	31	theme	detection	714:722	arg1	capable					688:694	capable	688:694	capable	688:694	The method was found to be simple, reliable and capable of high-sensitive detection of human serum albumin as a model, down to 0.2 pg/ml in the range of 0.25-1000 pg/ml.
26766869	2	32	theme	Au	280:281	arg1	oxide					316:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide	274:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	32	theme	Au	280:281	arg1	Fe3O4					323:327	Fe3O4	323:327	Fe3O4	323:327	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	32	theme	Au	280:281	arg1	shell/shell/core					331:346	shell/shell/core	331:346	shell/shell/core	331:346	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	0	33	theme	magnetic	73:80	arg1	tag					118:120	a tracer tag	109:120	a tracer tag for high-sensitive electrochemical detection	109:165	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	0	33	theme	magnetic	73:80	arg1	nanoparticles					92:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	6	34	theme	various	908:914	arg1	analytes					916:923	various analytes	908:923	various analytes	908:923	Findings of the present study would create new opportunities for sensitive and rapid detection of various analytes.
26766869	0	35	theme	oxide	67:71	arg1	tag					118:120	a tracer tag	109:120	a tracer tag for high-sensitive electrochemical detection	109:165	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	0	35	theme	oxide	67:71	arg1	nanoparticles					92:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	2	36	theme	Gold	274:277	arg1	oxide					316:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide	274:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	36	theme	Gold	274:277	arg1	Fe3O4					323:327	Fe3O4	323:327	Fe3O4	323:327	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	36	theme	Gold	274:277	arg1	shell/shell/core					331:346	shell/shell/core	331:346	shell/shell/core	331:346	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	37	theme	TMC	306:308	arg1	oxide					316:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide	274:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	37	theme	TMC	306:308	arg1	Fe3O4					323:327	Fe3O4	323:327	Fe3O4	323:327	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	37	theme	TMC	306:308	arg1	shell/shell/core					331:346	shell/shell/core	331:346	shell/shell/core	331:346	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	5	38	theme	serum	733:737	arg1	albumin					739:745	human serum albumin	727:745	human serum albumin	727:745	The method was found to be simple, reliable and capable of high-sensitive detection of human serum albumin as a model, down to 0.2 pg/ml in the range of 0.25-1000 pg/ml.
26766869	3	39	theme	high-density	480:491	arg1	assembly					493:500	high-density assembly	480:500	high-density assembly of Au nanoparticles (NPs) on TMC/Fe3O4 particles	480:549	The tag was shown to be capable of amplifying the recognition signal by high-density assembly of Au nanoparticles (NPs) on TMC/Fe3O4 particles.
26766869	4	40	theme	feasible	600:607	arg1	pathway					609:615	a feasible pathway	598:615	a feasible pathway for electron transfer	598:637	The remarkable conductivity of AuNPs provides a feasible pathway for electron transfer.
26766869	2	41	theme	chitosan	296:303	arg1	oxide					316:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide	274:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	41	theme	chitosan	296:303	arg1	Fe3O4					323:327	Fe3O4	323:327	Fe3O4	323:327	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	41	theme	chitosan	296:303	arg1	shell/shell/core					331:346	shell/shell/core	331:346	shell/shell/core	331:346	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	3	42	theme	recognition	458:468	arg1	signal					470:475	the recognition signal	454:475	the recognition signal	454:475	The tag was shown to be capable of amplifying the recognition signal by high-density assembly of Au nanoparticles (NPs) on TMC/Fe3O4 particles.
26766869	4	43	theme	electron	621:628	arg1	transfer					630:637	electron transfer	621:637	electron transfer	621:637	The remarkable conductivity of AuNPs provides a feasible pathway for electron transfer.
26766869	0	44	theme	composite	82:90	arg1	tag					118:120	a tracer tag	109:120	a tracer tag for high-sensitive electrochemical detection	109:165	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	0	44	theme	composite	82:90	arg1	nanoparticles					92:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles	36:104	Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection.
26766869	6	45	theme	rapid	889:893	arg1	detection					895:903	sensitive and rapid detection	875:903	sensitive and rapid detection of various analytes	875:923	Findings of the present study would create new opportunities for sensitive and rapid detection of various analytes.
26766869	2	46	theme	/N-trimethyl	283:294	arg1	oxide					316:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide	274:320	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	46	theme	/N-trimethyl	283:294	arg1	Fe3O4					323:327	Fe3O4	323:327	Fe3O4	323:327	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	46	theme	/N-trimethyl	283:294	arg1	shell/shell/core					331:346	shell/shell/core	331:346	shell/shell/core	331:346	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	47	theme	tracing	377:383	arg1	nanocomposite					349:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite	274:361	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	2	47	theme	tracing	377:383	arg1	tag					385:387	a tracing tag	375:387	a tracing tag to label antibody	375:405	Gold (Au)/N-trimethyl chitosan (TMC)/iron oxide (Fe3O4) (shell/shell/core) nanocomposite was used as a tracing tag to label antibody.
26766869	5	48	theme	human	727:731	arg1	albumin					739:745	human serum albumin	727:745	human serum albumin	727:745	The method was found to be simple, reliable and capable of high-sensitive detection of human serum albumin as a model, down to 0.2 pg/ml in the range of 0.25-1000 pg/ml.
25994595	8	0	theme	integrated	1330:1339	arg1	layer					1296:1300	a cartilage layer	1284:1300	a cartilage layer well developed and strongly integrated	1284:1339	By having a cartilage layer well developed and strongly integrated to the subchondral layer, OC tissues generated with NC may successfully engraft in an inflammatory post-surgery joint environment.
25994595	6	1	theme	constructs	1085:1094	arg1	analyzes					1066:1073	dGEMRIC and biochemical analyzes	1042:1073	dGEMRIC and biochemical analyzes of the OC constructs	1042:1094	dGEMRIC and biochemical analyzes of the OC constructs showed a reduced glycosaminoglycan (GAG) contents upon IL-1β administration.
25994595	8	2	theme	joint	1453:1457	arg1	environment					1459:1469	an inflammatory post-surgery joint environment	1424:1469	an inflammatory post-surgery joint environment	1424:1469	By having a cartilage layer well developed and strongly integrated to the subchondral layer, OC tissues generated with NC may successfully engraft in an inflammatory post-surgery joint environment.
25994595	2	3	theme	Chondro-Gide®	465:477	arg1	matrices					479:486	Chondro-Gide® matrices	465:486	Chondro-Gide® matrices	465:486	OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices) with devitalized spongiosa cylinders (Tutobone®).
25994595	4	4	theme	maturation	663:672	arg1	extent					674:679	Cartilage maturation extent	653:679	Cartilage maturation extent	653:679	Cartilage maturation extent was assessed (immune) histologically, biochemically and by delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test.
25994595	1	5	theme	lesions	243:249	arg1	repair					214:219	the repair	210:219	the repair of osteochondral (OC) lesions	210:249	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	7	6	theme	integration	1207:1217	arg1	forces					1219:1224	integration forces	1207:1224	integration forces	1207:1224	Cartilaginous matrix contents and integration forces returned to baseline up on withdrawal of IL-1β.
25994595	4	7	theme	peel-off	872:879	arg1	test					892:895	a peel-off mechanical test	870:895	a peel-off mechanical test	870:895	Cartilage maturation extent was assessed (immune) histologically, biochemically and by delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test.
25994595	5	8	dep	use	902:904	arg1	compared					915:922	compared	915:922	compared to AC	915:928	The use of NC as compared to AC allowed for more efficient cartilage matrix accumulation and superior integration of the cartilage/bone layers.
25994595	8	9	theme	inflammatory	1427:1438	arg1	environment					1459:1469	an inflammatory post-surgery joint environment	1424:1469	an inflammatory post-surgery joint environment	1424:1469	By having a cartilage layer well developed and strongly integrated to the subchondral layer, OC tissues generated with NC may successfully engraft in an inflammatory post-surgery joint environment.
25994595	3	10	theme	OC	539:540	arg1	tissues					542:548	OC tissues	539:548	OC tissues	539:548	OC tissues were then exposed to IL-1β for three days and cultured for additional 2 weeks in the absence of IL-1β.
25994595	2	11	theme	spongiosa	506:514	arg1	Tutobone®					527:535	Tutobone®	527:535	Tutobone®	527:535	OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices) with devitalized spongiosa cylinders (Tutobone®).
25994595	2	11	theme	spongiosa	506:514	arg1	cylinders					516:524	devitalized spongiosa cylinders	494:524	devitalized spongiosa cylinders (Tutobone®)	494:536	OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices) with devitalized spongiosa cylinders (Tutobone®).
25994595	4	12	theme	cartilage/bone	824:837	arg1	integration					839:849	cartilage/bone integration	824:849	cartilage/bone integration	824:849	Cartilage maturation extent was assessed (immune) histologically, biochemically and by delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test.
25994595	1	13	used	used	142:145	arg2	chondrocytes					117:128	nasal chondrocytes	111:128	nasal chondrocytes (NC)	111:133	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	1	13	used	used	142:145	arg2	NC					131:132	NC	131:132	NC	131:132	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	1	14	theme	composite	159:167	arg1	constructs					169:178	composite constructs	159:178	composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst	159:329	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	2	15	theme	devitalized	494:504	arg1	Tutobone®					527:535	Tutobone®	527:535	Tutobone®	527:535	OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices) with devitalized spongiosa cylinders (Tutobone®).
25994595	2	15	theme	devitalized	494:504	arg1	cylinders					516:524	devitalized spongiosa cylinders	494:524	devitalized spongiosa cylinders (Tutobone®)	494:536	OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices) with devitalized spongiosa cylinders (Tutobone®).
25994595	6	16	theme	glycosaminoglycan	1113:1129	arg1	contents					1137:1144	a reduced glycosaminoglycan (GAG) contents	1103:1144	a reduced glycosaminoglycan (GAG) contents	1103:1144	dGEMRIC and biochemical analyzes of the OC constructs showed a reduced glycosaminoglycan (GAG) contents upon IL-1β administration.
25994595	4	17	theme	delayed	740:746	arg1	resonance					777:785	delayed gadolinium-enhanced magnetic resonance	740:785	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test	740:895	Cartilage maturation extent was assessed (immune) histologically, biochemically and by delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test.
25994595	2	18	theme	cartilage	382:390	arg1	tissues					392:398	engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices)	371:487	engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices)	371:487	OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices) with devitalized spongiosa cylinders (Tutobone®).
25994595	7	19	theme	Cartilaginous	1173:1185	arg1	contents					1194:1201	Cartilaginous matrix contents	1173:1201	Cartilaginous matrix contents	1173:1201	Cartilaginous matrix contents and integration forces returned to baseline up on withdrawal of IL-1β.
25994595	6	20	theme	IL-1β	1151:1155	arg1	administration					1157:1170	IL-1β administration	1151:1170	IL-1β administration	1151:1170	dGEMRIC and biochemical analyzes of the OC constructs showed a reduced glycosaminoglycan (GAG) contents upon IL-1β administration.
25994595	2	21	theme	engineered	371:380	arg1	tissues					392:398	engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices)	371:487	engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices)	371:487	OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices) with devitalized spongiosa cylinders (Tutobone®).
25994595	2	22	theme	articular	430:438	arg1	chondrocytes					440:451	articular chondrocytes	430:451	articular chondrocytes	430:451	OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices) with devitalized spongiosa cylinders (Tutobone®).
25994595	8	23	theme	OC	1367:1368	arg1	tissues					1370:1376	OC tissues	1367:1376	OC tissues generated with NC	1367:1394	By having a cartilage layer well developed and strongly integrated to the subchondral layer, OC tissues generated with NC may successfully engraft in an inflammatory post-surgery joint environment.
25994595	6	24	theme	biochemical	1054:1064	arg1	analyzes					1066:1073	dGEMRIC and biochemical analyzes	1042:1073	dGEMRIC and biochemical analyzes of the OC constructs	1042:1094	dGEMRIC and biochemical analyzes of the OC constructs showed a reduced glycosaminoglycan (GAG) contents upon IL-1β administration.
25994595	8	25	theme	post-surgery	1440:1451	arg1	environment					1459:1469	an inflammatory post-surgery joint environment	1424:1469	an inflammatory post-surgery joint environment	1424:1469	By having a cartilage layer well developed and strongly integrated to the subchondral layer, OC tissues generated with NC may successfully engraft in an inflammatory post-surgery joint environment.
25994595	1	26	theme	nasal	111:115	arg1	NC					131:132	NC	131:132	NC	131:132	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	1	26	theme	nasal	111:115	arg1	chondrocytes					117:128	nasal chondrocytes	111:128	nasal chondrocytes (NC)	111:133	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	1	27	theme	necessary	196:204	arg1	integration					271:281	integration	271:281	integration	271:281	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	1	27	theme	necessary	196:204	arg1	maturation					259:268	maturation	259:268	maturation	259:268	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	1	27	theme	necessary	196:204	arg1	properties					185:194	properties	185:194	properties necessary for the repair of osteochondral (OC) lesions	185:249	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	1	27	theme	necessary	196:204	arg1	capacity					287:294	capacity	287:294	capacity	287:294	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	4	28	theme	gadolinium-enhanced	748:766	arg1	resonance					777:785	delayed gadolinium-enhanced magnetic resonance	740:785	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test	740:895	Cartilage maturation extent was assessed (immune) histologically, biochemically and by delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test.
25994595	6	29	theme	OC	1082:1083	arg1	constructs					1085:1094	the OC constructs	1078:1094	the OC constructs	1078:1094	dGEMRIC and biochemical analyzes of the OC constructs showed a reduced glycosaminoglycan (GAG) contents upon IL-1β administration.
25994595	6	30	theme	dGEMRIC	1042:1048	arg1	analyzes					1066:1073	dGEMRIC and biochemical analyzes	1042:1073	dGEMRIC and biochemical analyzes of the OC constructs	1042:1094	dGEMRIC and biochemical analyzes of the OC constructs showed a reduced glycosaminoglycan (GAG) contents upon IL-1β administration.
25994595	0	31	theme	grafts	49:54	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Generation and characterization of osteochondral grafts with human nasal chondrocytes.
25994595	0	31	theme	grafts	49:54	arg1	Generation					0:9	Generation	0:9	Generation	0:9	Generation and characterization of osteochondral grafts with human nasal chondrocytes.
25994595	7	32	theme	matrix	1187:1192	arg1	contents					1194:1201	Cartilaginous matrix contents	1173:1201	Cartilaginous matrix contents	1173:1201	Cartilaginous matrix contents and integration forces returned to baseline up on withdrawal of IL-1β.
25994595	8	33	theme	developed	1307:1315	arg1	layer					1296:1300	a cartilage layer	1284:1300	a cartilage layer well developed and strongly integrated	1284:1339	By having a cartilage layer well developed and strongly integrated to the subchondral layer, OC tissues generated with NC may successfully engraft in an inflammatory post-surgery joint environment.
25994595	0	34	theme	osteochondral	35:47	arg1	grafts					49:54	osteochondral grafts	35:54	osteochondral grafts	35:54	Generation and characterization of osteochondral grafts with human nasal chondrocytes.
25994595	3	35	theme	additional	609:618	arg1	weeks					622:626	additional 2 weeks	609:626	additional 2 weeks	609:626	OC tissues were then exposed to IL-1β for three days and cultured for additional 2 weeks in the absence of IL-1β.
25994595	0	36	theme	human	61:65	arg1	chondrocytes					73:84	human nasal chondrocytes	61:84	human nasal chondrocytes	61:84	Generation and characterization of osteochondral grafts with human nasal chondrocytes.
25994595	8	37	theme	subchondral	1348:1358	arg1	layer					1360:1364	the subchondral layer	1344:1364	the subchondral layer	1344:1364	By having a cartilage layer well developed and strongly integrated to the subchondral layer, OC tissues generated with NC may successfully engraft in an inflammatory post-surgery joint environment.
25994595	0	38	with	characterization	15:30	arg1	chondrocytes					73:84	human nasal chondrocytes	61:84	human nasal chondrocytes	61:84	Generation and characterization of osteochondral grafts with human nasal chondrocytes.
25994595	2	39	theme	OC	332:333	arg1	grafts					335:340	OC grafts	332:340	OC grafts	332:340	OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices) with devitalized spongiosa cylinders (Tutobone®).
25994595	4	40	theme	mechanical	881:890	arg1	test					892:895	a peel-off mechanical test	870:895	a peel-off mechanical test	870:895	Cartilage maturation extent was assessed (immune) histologically, biochemically and by delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test.
25994595	5	41	theme	layers	1034:1039	arg1	accumulation					974:985	more efficient cartilage matrix accumulation	942:985	more efficient cartilage matrix accumulation	942:985	The use of NC as compared to AC allowed for more efficient cartilage matrix accumulation and superior integration of the cartilage/bone layers.
25994595	5	41	theme	layers	1034:1039	arg1	integration					1000:1010	superior integration	991:1010	superior integration of the cartilage/bone layers	991:1039	The use of NC as compared to AC allowed for more efficient cartilage matrix accumulation and superior integration of the cartilage/bone layers.
25994595	5	42	theme	cartilage/bone	1019:1032	arg1	layers					1034:1039	the cartilage/bone layers	1015:1039	the cartilage/bone layers	1015:1039	The use of NC as compared to AC allowed for more efficient cartilage matrix accumulation and superior integration of the cartilage/bone layers.
25994595	8	43	theme	cartilage	1286:1294	arg1	layer					1296:1300	a cartilage layer	1284:1300	a cartilage layer well developed and strongly integrated	1284:1339	By having a cartilage layer well developed and strongly integrated to the subchondral layer, OC tissues generated with NC may successfully engraft in an inflammatory post-surgery joint environment.
25994595	7	44	theme	IL-1β	1267:1271	arg1	withdrawal					1253:1262	withdrawal	1253:1262	withdrawal of IL-1β	1253:1271	Cartilaginous matrix contents and integration forces returned to baseline up on withdrawal of IL-1β.
25994595	3	45	theme	IL-1β	646:650	arg1	absence					635:641	the absence	631:641	the absence of IL-1β	631:650	OC tissues were then exposed to IL-1β for three days and cultured for additional 2 weeks in the absence of IL-1β.
25994595	5	46	theme	matrix	967:972	arg1	accumulation					974:985	more efficient cartilage matrix accumulation	942:985	more efficient cartilage matrix accumulation	942:985	The use of NC as compared to AC allowed for more efficient cartilage matrix accumulation and superior integration of the cartilage/bone layers.
25994595	0	47	with	Generation	0:9	arg1	chondrocytes					73:84	human nasal chondrocytes	61:84	human nasal chondrocytes	61:84	Generation and characterization of osteochondral grafts with human nasal chondrocytes.
25994595	5	48	theme	NC	909:910	arg1	use					902:904	The use	898:904	The use of NC as compared to AC	898:928	The use of NC as compared to AC allowed for more efficient cartilage matrix accumulation and superior integration of the cartilage/bone layers.
25994595	4	49	dep	assessed	685:692	arg1	immune					695:700	immune	695:700	immune	695:700	Cartilage maturation extent was assessed (immune) histologically, biochemically and by delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test.
25994595	1	50	with	constructs	169:178	arg1	integration					271:281	integration	271:281	integration	271:281	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	1	50	with	constructs	169:178	arg1	maturation					259:268	maturation	259:268	maturation	259:268	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	1	50	with	constructs	169:178	arg1	properties					185:194	properties	185:194	properties necessary for the repair of osteochondral (OC) lesions	185:249	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	1	50	with	constructs	169:178	arg1	capacity					287:294	capacity	287:294	capacity	287:294	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	4	51	theme	magnetic	768:775	arg1	resonance					777:785	delayed gadolinium-enhanced magnetic resonance	740:785	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test	740:895	Cartilage maturation extent was assessed (immune) histologically, biochemically and by delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test.
25994595	6	52	theme	reduced	1105:1111	arg1	glycosaminoglycan					1113:1129	a reduced glycosaminoglycan	1103:1129	a reduced glycosaminoglycan (GAG) contents	1103:1144	dGEMRIC and biochemical analyzes of the OC constructs showed a reduced glycosaminoglycan (GAG) contents upon IL-1β administration.
25994595	6	52	theme	reduced	1105:1111	arg1	GAG					1132:1134	GAG	1132:1134	GAG	1132:1134	dGEMRIC and biochemical analyzes of the OC constructs showed a reduced glycosaminoglycan (GAG) contents upon IL-1β administration.
25994595	1	53	theme	osteochondral	224:236	arg1	lesions					243:249	osteochondral (OC) lesions	224:249	osteochondral (OC) lesions	224:249	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	1	53	theme	osteochondral	224:236	arg1	OC					239:240	OC	239:240	OC	239:240	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	7	54	theme	baseline	1238:1245	arg1	up					1247:1248	baseline up	1238:1248	baseline up	1238:1248	Cartilaginous matrix contents and integration forces returned to baseline up on withdrawal of IL-1β.
25994595	5	55	theme	efficient	947:955	arg1	accumulation					974:985	more efficient cartilage matrix accumulation	942:985	more efficient cartilage matrix accumulation	942:985	The use of NC as compared to AC allowed for more efficient cartilage matrix accumulation and superior integration of the cartilage/bone layers.
25994595	2	56	dep	tissues	392:398	arg1	generated					401:409	generated	401:409	generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices	401:486	OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes - AC - onto Chondro-Gide® matrices) with devitalized spongiosa cylinders (Tutobone®).
25994595	4	57	theme	Cartilage	653:661	arg1	extent					674:679	Cartilage maturation extent	653:679	Cartilage maturation extent	653:679	Cartilage maturation extent was assessed (immune) histologically, biochemically and by delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel-off mechanical test.
25994595	0	58	theme	nasal	67:71	arg1	chondrocytes					73:84	human nasal chondrocytes	61:84	human nasal chondrocytes	61:84	Generation and characterization of osteochondral grafts with human nasal chondrocytes.
25994595	1	59	theme	inflammatory	312:323	arg1	burst					325:329	inflammatory burst	312:329	inflammatory burst	312:329	We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst.
25994595	5	60	theme	superior	991:998	arg1	integration					1000:1010	superior integration	991:1010	superior integration of the cartilage/bone layers	991:1039	The use of NC as compared to AC allowed for more efficient cartilage matrix accumulation and superior integration of the cartilage/bone layers.
25994595	5	61	theme	cartilage	957:965	arg1	accumulation					974:985	more efficient cartilage matrix accumulation	942:985	more efficient cartilage matrix accumulation	942:985	The use of NC as compared to AC allowed for more efficient cartilage matrix accumulation and superior integration of the cartilage/bone layers.
28854241	2	0	theme	delaminated	329:339	arg1	nanosheet					347:355	delaminated MXene nanosheet	329:355	delaminated MXene nanosheet	329:355	The high aspect ratio, hydrophilic surfaces, and metallic conductivity of delaminated MXene nanosheet render it promising nanofiller for high performance nanocomposites.
28854241	6	1	theme	dc	865:866	arg1	conductivity					868:879	dc conductivity	865:879	dc conductivity of 0.8 mS/cm conductivity which is superior compared to similar composites prepared using methods other than electrospinning	865:1004	% Ti3C2Tx exhibited dc conductivity of 0.8 mS/cm conductivity which is superior compared to similar composites prepared using methods other than electrospinning.
28854241	1	2	theme	MXene	135:139	arg1	nanofibers					177:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers have been successfully fabricated by an electrospinning technique.
28854241	0	3	theme	enhanced	70:77	arg1	properties					105:114	enhanced mechanical and electrical properties	70:114	enhanced mechanical and electrical properties	70:114	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	5	4	theme	Dynamic	635:641	arg1	analysis					654:661	Dynamic mechanical analysis	635:661	Dynamic mechanical analysis	635:661	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	5	5	contain	containing	825:834	arg1	nanofibers					814:823	PVA nanofibers	810:823	PVA nanofibers containing 0.14 wt	810:842	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	5	5	contain	containing	825:834	arg1	MPa					751:753	855 MPa	747:753	855 MPa for fibers containing CNC and MXene at 25°C. Moreover	747:807	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	5	5	contain	containing	825:834	arg2	wt					841:842	0.14 wt	836:842	0.14 wt	836:842	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	2	6	theme	aspect	264:269	arg1	ratio					271:275	The high aspect ratio	255:275	The high aspect ratio	255:275	The high aspect ratio, hydrophilic surfaces, and metallic conductivity of delaminated MXene nanosheet render it promising nanofiller for high performance nanocomposites.
28854241	1	7	theme	electrospinning	228:242	arg1	technique					244:252	an electrospinning technique	225:252	an electrospinning technique	225:252	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers have been successfully fabricated by an electrospinning technique.
28854241	5	8	theme	mechanical	643:652	arg1	analysis					654:661	Dynamic mechanical analysis	635:661	Dynamic mechanical analysis	635:661	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	6	9	theme	mS/cm	888:892	arg1	conductivity					894:905	0.8 mS/cm conductivity	884:905	0.8 mS/cm conductivity	884:905	% Ti3C2Tx exhibited dc conductivity of 0.8 mS/cm conductivity which is superior compared to similar composites prepared using methods other than electrospinning.
28854241	2	10	theme	high	259:262	arg1	ratio					271:275	The high aspect ratio	255:275	The high aspect ratio	255:275	The high aspect ratio, hydrophilic surfaces, and metallic conductivity of delaminated MXene nanosheet render it promising nanofiller for high performance nanocomposites.
28854241	6	11	theme	similar	937:943	arg1	composites					945:954	similar composites	937:954	similar composites prepared using methods other than electrospinning	937:1004	% Ti3C2Tx exhibited dc conductivity of 0.8 mS/cm conductivity which is superior compared to similar composites prepared using methods other than electrospinning.
28854241	6	12	theme	%	845:845	arg1	Ti3C2Tx					847:853	% Ti3C2Tx	845:853	% Ti3C2Tx	845:853	% Ti3C2Tx exhibited dc conductivity of 0.8 mS/cm conductivity which is superior compared to similar composites prepared using methods other than electrospinning.
28854241	7	13	theme	composites	1082:1091	arg1	characteristics					1042:1056	Improved mechanical and electrical characteristics	1007:1056	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites	1007:1091	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites make them viable materials for high performance energy applications.
28854241	5	14	theme	PVA	810:812	arg1	nanofibers					814:823	PVA nanofibers	810:823	PVA nanofibers containing 0.14 wt	810:842	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	5	14	theme	PVA	810:812	arg1	MPa					751:753	855 MPa	747:753	855 MPa for fibers containing CNC and MXene at 25°C. Moreover	747:807	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	0	15	theme	mechanical	79:88	arg1	properties					105:114	enhanced mechanical and electrical properties	70:114	enhanced mechanical and electrical properties	70:114	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	7	16	theme	energy	1141:1146	arg1	applications					1148:1159	high performance energy applications	1124:1159	high performance energy applications	1124:1159	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites make them viable materials for high performance energy applications.
28854241	1	17	theme	-reinforced	141:151	arg1	nanofibers					177:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers have been successfully fabricated by an electrospinning technique.
28854241	0	18	theme	Ti3C2Tx	3:9	arg1	PVA					49:51	PVA	49:51	PVA	49:51	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	0	18	theme	Ti3C2Tx	3:9	arg1	alcohol					40:46	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol	0:46	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA)	0:52	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	3	19	theme	nanofibers	508:517	arg1	properties					490:499	the mechanical properties	475:499	the mechanical properties of the nanofibers	475:517	Cellulose nanocrystals (CNC) were used to improve the mechanical properties of the nanofibers.
28854241	7	20	theme	Ti3C2Tx	1065:1071	arg1	composites					1082:1091	the Ti3C2Tx /CNC/PVA composites	1061:1091	the Ti3C2Tx /CNC/PVA composites	1061:1091	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites make them viable materials for high performance energy applications.
28854241	1	21	theme	polyvinyl	153:161	arg1	PVA					172:174	PVA	172:174	PVA	172:174	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers have been successfully fabricated by an electrospinning technique.
28854241	1	21	theme	polyvinyl	153:161	arg1	alcohol					163:169	polyvinyl alcohol	153:169	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers have been successfully fabricated by an electrospinning technique.
28854241	0	22	theme	2D	0:1	arg1	PVA					49:51	PVA	49:51	PVA	49:51	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	0	22	theme	2D	0:1	arg1	alcohol					40:46	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol	0:46	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA)	0:52	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	7	23	theme	/CNC/PVA	1073:1080	arg1	composites					1082:1091	the Ti3C2Tx /CNC/PVA composites	1061:1091	the Ti3C2Tx /CNC/PVA composites	1061:1091	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites make them viable materials for high performance energy applications.
28854241	2	24	theme	hydrophilic	278:288	arg1	surfaces					290:297	hydrophilic surfaces	278:297	hydrophilic surfaces	278:297	The high aspect ratio, hydrophilic surfaces, and metallic conductivity of delaminated MXene nanosheet render it promising nanofiller for high performance nanocomposites.
28854241	1	25	theme	alcohol	163:169	arg1	nanofibers					177:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers have been successfully fabricated by an electrospinning technique.
28854241	0	26	theme	MXene	12:16	arg1	PVA					49:51	PVA	49:51	PVA	49:51	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	0	26	theme	MXene	12:16	arg1	alcohol					40:46	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol	0:46	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA)	0:52	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	7	27	theme	viable	1103:1108	arg1	materials					1110:1118	viable materials	1103:1118	viable materials for high performance energy applications	1103:1159	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites make them viable materials for high performance energy applications.
28854241	3	28	used	used	459:462	arg2	CNC					449:451	CNC	449:451	CNC	449:451	Cellulose nanocrystals (CNC) were used to improve the mechanical properties of the nanofibers.
28854241	3	28	used	used	459:462	arg2	nanocrystals					435:446	Cellulose nanocrystals	425:446	Cellulose nanocrystals (CNC)	425:452	Cellulose nanocrystals (CNC) were used to improve the mechanical properties of the nanofibers.
28854241	7	29	theme	electrical	1031:1040	arg1	characteristics					1042:1056	Improved mechanical and electrical characteristics	1007:1056	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites	1007:1091	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites make them viable materials for high performance energy applications.
28854241	0	30	theme	electrical	94:103	arg1	properties					105:114	enhanced mechanical and electrical properties	70:114	enhanced mechanical and electrical properties	70:114	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	5	31	from	MPa	720:722	arg1	increase					679:686	an increase	676:686	an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt	676:842	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	5	31	from	MPa	720:722	arg1	modulus					703:709	the elastic modulus	691:709	the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt	691:842	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	4	32	contain	had	556:558	arg2	diameter					560:567	diameter	560:567	diameter from 174 to 194 nm	560:586	The obtained electrospun nanofibers had diameter from 174 to 194 nm depending on ratio between PVA, CNC and MXene.
28854241	4	32	contain	had	556:558	arg1	nanofibers					545:554	The obtained electrospun nanofibers	520:554	The obtained electrospun nanofibers	520:554	The obtained electrospun nanofibers had diameter from 174 to 194 nm depending on ratio between PVA, CNC and MXene.
28854241	2	33	theme	performance	397:407	arg1	nanocomposites					409:422	high performance nanocomposites	392:422	high performance nanocomposites	392:422	The high aspect ratio, hydrophilic surfaces, and metallic conductivity of delaminated MXene nanosheet render it promising nanofiller for high performance nanocomposites.
28854241	0	34	theme	-reinforced	18:28	arg1	PVA					49:51	PVA	49:51	PVA	49:51	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	0	34	theme	-reinforced	18:28	arg1	alcohol					40:46	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol	0:46	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA)	0:52	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	3	35	theme	mechanical	479:488	arg1	properties					490:499	the mechanical properties	475:499	the mechanical properties of the nanofibers	475:517	Cellulose nanocrystals (CNC) were used to improve the mechanical properties of the nanofibers.
28854241	5	36	theme	elastic	695:701	arg1	modulus					703:709	the elastic modulus	691:709	the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt	691:842	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	4	37	dep	194	581:583	arg1	to					578:579	to	578:579	to	578:579	The obtained electrospun nanofibers had diameter from 174 to 194 nm depending on ratio between PVA, CNC and MXene.
28854241	3	38	theme	Cellulose	425:433	arg1	nanocrystals					435:446	Cellulose nanocrystals	425:446	Cellulose nanocrystals (CNC)	425:452	Cellulose nanocrystals (CNC) were used to improve the mechanical properties of the nanofibers.
28854241	3	38	theme	Cellulose	425:433	arg1	CNC					449:451	CNC	449:451	CNC	449:451	Cellulose nanocrystals (CNC) were used to improve the mechanical properties of the nanofibers.
28854241	2	39	theme	high	392:395	arg1	nanocomposites					409:422	high performance nanocomposites	392:422	high performance nanocomposites	392:422	The high aspect ratio, hydrophilic surfaces, and metallic conductivity of delaminated MXene nanosheet render it promising nanofiller for high performance nanocomposites.
28854241	6	40	theme	0.8	884:886	arg1	mS/cm					888:892	mS/cm	888:892	mS/cm	888:892	% Ti3C2Tx exhibited dc conductivity of 0.8 mS/cm conductivity which is superior compared to similar composites prepared using methods other than electrospinning.
28854241	7	41	theme	high	1124:1127	arg1	applications					1148:1159	high performance energy applications	1124:1159	high performance energy applications	1124:1159	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites make them viable materials for high performance energy applications.
28854241	4	42	from	nm	585:586	arg1	diameter					560:567	diameter	560:567	diameter from 174 to 194 nm	560:586	The obtained electrospun nanofibers had diameter from 174 to 194 nm depending on ratio between PVA, CNC and MXene.
28854241	7	43	theme	mechanical	1016:1025	arg1	characteristics					1042:1056	Improved mechanical and electrical characteristics	1007:1056	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites	1007:1091	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites make them viable materials for high performance energy applications.
28854241	5	44	contain	containing	766:775	arg2	MXene					785:789	MXene	785:789	MXene	785:789	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	5	44	contain	containing	766:775	arg2	CNC					777:779	CNC	777:779	CNC	777:779	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	5	44	contain	containing	766:775	arg1	fibers					759:764	fibers	759:764	fibers containing CNC and MXene at 25°C. Moreover	759:807	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	0	45	theme	polyvinyl	30:38	arg1	PVA					49:51	PVA	49:51	PVA	49:51	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	0	45	theme	polyvinyl	30:38	arg1	alcohol					40:46	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol	0:46	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA)	0:52	2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers with enhanced mechanical and electrical properties.
28854241	4	46	theme	electrospun	533:543	arg1	nanofibers					545:554	The obtained electrospun nanofibers	520:554	The obtained electrospun nanofibers	520:554	The obtained electrospun nanofibers had diameter from 174 to 194 nm depending on ratio between PVA, CNC and MXene.
28854241	1	47	theme	Ti3C2Tx	126:132	arg1	nanofibers					177:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers have been successfully fabricated by an electrospinning technique.
28854241	2	48	theme	metallic	304:311	arg1	conductivity					313:324	metallic conductivity	304:324	metallic conductivity of delaminated MXene nanosheet	304:355	The high aspect ratio, hydrophilic surfaces, and metallic conductivity of delaminated MXene nanosheet render it promising nanofiller for high performance nanocomposites.
28854241	4	49	theme	obtained	524:531	arg1	nanofibers					545:554	The obtained electrospun nanofibers	520:554	The obtained electrospun nanofibers	520:554	The obtained electrospun nanofibers had diameter from 174 to 194 nm depending on ratio between PVA, CNC and MXene.
28854241	1	50	theme	2D	123:124	arg1	nanofibers					177:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers have been successfully fabricated by an electrospinning technique.
28854241	7	51	theme	performance	1129:1139	arg1	applications					1148:1159	high performance energy applications	1124:1159	high performance energy applications	1124:1159	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites make them viable materials for high performance energy applications.
28854241	7	52	theme	Improved	1007:1014	arg1	characteristics					1042:1056	Improved mechanical and electrical characteristics	1007:1056	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites	1007:1091	Improved mechanical and electrical characteristics of the Ti3C2Tx /CNC/PVA composites make them viable materials for high performance energy applications.
28854241	1	53	theme	Novel	117:121	arg1	nanofibers					177:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers	117:186	Novel 2D Ti3C2Tx (MXene)-reinforced polyvinyl alcohol (PVA) nanofibers have been successfully fabricated by an electrospinning technique.
28854241	5	54	from	increase	679:686	arg1	MPa					720:722	392 MPa	716:722	392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt	716:842	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	5	54	from	increase	679:686	arg1	modulus					703:709	the elastic modulus	691:709	the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt	691:842	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	5	55	theme	neat	728:731	arg1	fibers					737:742	neat PVA fibers	728:742	neat PVA fibers	728:742	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	2	56	theme	nanosheet	347:355	arg1	conductivity					313:324	metallic conductivity	304:324	metallic conductivity of delaminated MXene nanosheet	304:355	The high aspect ratio, hydrophilic surfaces, and metallic conductivity of delaminated MXene nanosheet render it promising nanofiller for high performance nanocomposites.
28854241	2	56	theme	nanosheet	347:355	arg1	ratio					271:275	The high aspect ratio	255:275	The high aspect ratio	255:275	The high aspect ratio, hydrophilic surfaces, and metallic conductivity of delaminated MXene nanosheet render it promising nanofiller for high performance nanocomposites.
28854241	2	56	theme	nanosheet	347:355	arg1	surfaces					290:297	hydrophilic surfaces	278:297	hydrophilic surfaces	278:297	The high aspect ratio, hydrophilic surfaces, and metallic conductivity of delaminated MXene nanosheet render it promising nanofiller for high performance nanocomposites.
28854241	6	57	theme	other	979:983	arg1	methods					971:977	methods	971:977	methods other than electrospinning	971:1004	% Ti3C2Tx exhibited dc conductivity of 0.8 mS/cm conductivity which is superior compared to similar composites prepared using methods other than electrospinning.
28854241	5	58	theme	PVA	733:735	arg1	fibers					737:742	neat PVA fibers	728:742	neat PVA fibers	728:742	Dynamic mechanical analysis demonstrated an increase in the elastic modulus from 392 MPa for neat PVA fibers to 855 MPa for fibers containing CNC and MXene at 25°C. Moreover, PVA nanofibers containing 0.14 wt.
28854241	2	59	theme	MXene	341:345	arg1	nanosheet					347:355	delaminated MXene nanosheet	329:355	delaminated MXene nanosheet	329:355	The high aspect ratio, hydrophilic surfaces, and metallic conductivity of delaminated MXene nanosheet render it promising nanofiller for high performance nanocomposites.
28854241	6	60	theme	conductivity	894:905	arg1	conductivity					868:879	dc conductivity	865:879	dc conductivity of 0.8 mS/cm conductivity which is superior compared to similar composites prepared using methods other than electrospinning	865:1004	% Ti3C2Tx exhibited dc conductivity of 0.8 mS/cm conductivity which is superior compared to similar composites prepared using methods other than electrospinning.
26210277	2	0	dep	compositions	512:523	arg1	CHO					526:528	CHO	526:528	CHO; rich in starch vs. rich in fiber	526:562	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	2	1	theme	rich	531:534	arg1	CHO					526:528	CHO	526:528	CHO; rich in starch vs. rich in fiber	526:562	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	5	2	theme	protein	1340:1346	arg1	yield					1348:1352	a greater milk protein yield	1325:1352	a greater milk protein yield	1325:1352	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	9	3	theme	mammary	2076:2082	arg1	uptake					2084:2089	uptake	2084:2089	uptake	2084:2089	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	1	4	theme	dietary	362:368	arg1	content					389:395	dietary crude protein (CP) content	362:395	dietary crude protein (CP) content	362:395	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	1	5	theme	feeding	205:211	arg1	consequence					256:266	the consequence	252:266	the consequence of a higher efficiency of AA use	252:299	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	1	5	theme	feeding	205:211	arg1	diets					213:217	feeding diets	205:217	feeding diets based on starch versus fiber	205:246	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	8	6	theme	BCAA	1927:1930	arg1	uptake					1917:1922	uptake	1917:1922	uptake of BCAA observed in this study	1917:1953	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	8	6	theme	BCAA	1927:1930	arg1	rate					1908:1911	mammary clearance rate	1890:1911	mammary clearance rate	1890:1911	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	4	7	theme	fiber	902:906	arg1	diets					908:912	fiber diets	902:912	fiber diets	902:912	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	1	8	theme	protein	376:382	arg1	content					389:395	dietary crude protein (CP) content	362:395	dietary crude protein (CP) content	362:395	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	7	9	theme	clearance	1679:1687	arg1	rate					1689:1692	clearance rate	1679:1692	clearance rate	1679:1692	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	2	10	theme	rich	550:553	arg1	CHO					526:528	CHO	526:528	CHO; rich in starch vs. rich in fiber	526:562	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	7	11	theme	effects	1773:1779	arg1	absence					1762:1768	absence	1762:1768	absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism	1762:1848	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	8	12	dep	16.5	2016:2019	arg1	to					2013:2014	to	2013:2014	to	2013:2014	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	5	13	from	average	1361:1367	arg1	uptake					1145:1150	the mammary net uptake	1129:1150	the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level	1129:1399	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	4	14	theme	starch	973:978	arg1	diets					980:984	starch diets	973:984	starch diets	973:984	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	10	15	theme	AA	2320:2321	arg1	utilization					2323:2333	the mammary AA utilization	2308:2333	the mammary AA utilization	2308:2333	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	7	16	theme	greater	1645:1651	arg1	extraction					1664:1673	a greater fractional extraction	1643:1673	a greater fractional extraction	1643:1673	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	0	17	theme	lactating	87:95	arg1	cows					104:107	lactating Jersey cows	87:107	lactating Jersey cows	87:107	Diets rich in starch improve the efficiency of amino acids use by the mammary gland in lactating Jersey cows.
26210277	1	18	theme	mammary	312:318	arg1	gland					320:324	the mammary gland	308:324	the mammary gland	308:324	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	7	19	from	flow	1816:1819	arg1	absence					1762:1768	absence	1762:1768	absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism	1762:1848	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	11	20	theme	glucogenic	2629:2638	arg1	nutrients					2640:2648	glucogenic nutrients	2629:2648	glucogenic nutrients	2629:2648	Results from this study suggest that mammary Leu and glucose metabolism can be modulated by the supply of glucogenic nutrients to the mammary gland.
26210277	6	21	from	%	1505:1505	arg1	average					1510:1516	average	1510:1516	average	1510:1516	The conversion efficiency of plasma essential AA into milk protein was improved with starch diets (33.7% on average) compared with fiber diets (27.5% on average).
26210277	11	22	theme	mammary	2657:2663	arg1	gland					2665:2669	the mammary gland	2653:2669	the mammary gland	2653:2669	Results from this study suggest that mammary Leu and glucose metabolism can be modulated by the supply of glucogenic nutrients to the mammary gland.
26210277	1	23	theme	milk	170:173	arg1	N					175:175	the greater milk N	158:175	the greater milk N	158:175	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	9	24	theme	output	2218:2223	arg1	ratio					2225:2229	milk output ratio	2213:2229	milk output ratio	2213:2229	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	8	25	theme	dietary	1992:1998	arg1	CP					2000:2001	dietary CP	1992:2001	dietary CP	1992:2001	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	1	26	dep	yield	177:181	arg1	observed					191:198	observed	191:198	yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland	177:324	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	2	27	from	fiber	558:562	arg1	rich					550:553	rich	550:553	rich	550:553	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	9	28	from	diets	2150:2154	arg1	proportion					2114:2123	a higher proportion	2105:2123	a higher proportion of total essential AA in diets based on starch versus fiber	2105:2183	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	7	29	from	gland	1753:1757	arg1	absence					1762:1768	absence	1762:1768	absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism	1762:1848	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	10	30	theme	metabolic	2427:2435	arg1	pathways					2437:2444	different metabolic pathways	2417:2444	different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels	2417:2520	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	2	31	theme	different	489:497	arg1	compositions					512:523	2 different carbohydrate compositions	487:523	2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP)	487:621	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	0	32	from	gland	78:82	arg1	cows					104:107	lactating Jersey cows	87:107	lactating Jersey cows	87:107	Diets rich in starch improve the efficiency of amino acids use by the mammary gland in lactating Jersey cows.
26210277	5	33	from	uptake	1145:1150	arg1	average					1361:1367	average	1361:1367	average	1361:1367	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	4	34	theme	clearance	1018:1026	arg1	rate					1028:1031	clearance rate	1018:1031	clearance rate of glucose	1018:1042	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	7	35	with	use	1596:1598	arg1	diets					1618:1622	starch diets	1611:1622	starch diets	1611:1622	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	9	36	from	AA	2144:2145	arg1	diets					2150:2154	diets	2150:2154	diets based on starch versus fiber	2150:2183	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	7	37	from	absence	1762:1768	arg1	metabolism					1839:1848	the mammary AA metabolism	1824:1848	the mammary AA metabolism	1824:1848	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	7	37	from	absence	1762:1768	arg1	flow					1816:1819	the mammary blood flow	1798:1819	the mammary blood flow	1798:1819	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	11	38	theme	nutrients	2640:2648	arg1	supply					2619:2624	the supply	2615:2624	the supply of glucogenic nutrients to the mammary gland	2615:2669	Results from this study suggest that mammary Leu and glucose metabolism can be modulated by the supply of glucogenic nutrients to the mammary gland.
26210277	5	39	dep	enhanced	1220:1227	arg1	%					1244:1244	12, 11, and 26%	1230:1244	12, 11, and 26% on average	1230:1255	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	2	40	theme	Jersey	434:439	arg1	cows					441:444	Five midlactation multicatheterized Jersey cows	398:444	Five midlactation multicatheterized Jersey cows	398:444	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	0	41	theme	amino	47:51	arg1	acids					53:57	amino acids	47:57	amino acids	47:57	Diets rich in starch improve the efficiency of amino acids use by the mammary gland in lactating Jersey cows.
26210277	7	42	from	effects	1773:1779	arg1	metabolism					1839:1848	the mammary AA metabolism	1824:1848	the mammary AA metabolism	1824:1848	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	7	42	from	effects	1773:1779	arg1	flow					1816:1819	the mammary blood flow	1798:1819	the mammary blood flow	1798:1819	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	10	43	theme	starch	2455:2460	arg1	diets					2462:2466	starch diets	2455:2466	starch diets	2455:2466	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	2	44	theme	midlactation	403:414	arg1	cows					441:444	Five midlactation multicatheterized Jersey cows	398:444	Five midlactation multicatheterized Jersey cows	398:444	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	6	45	theme	milk	1456:1459	arg1	protein					1461:1467	milk protein	1456:1467	milk protein	1456:1467	The conversion efficiency of plasma essential AA into milk protein was improved with starch diets (33.7% on average) compared with fiber diets (27.5% on average).
26210277	4	46	theme	mammary	995:1001	arg1	uptake					1007:1012	mammary net uptake	995:1012	mammary net uptake	995:1012	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	1	47	theme	efficiency	280:289	arg1	diets					213:217	feeding diets	205:217	feeding diets based on starch versus fiber	205:246	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	1	47	theme	efficiency	280:289	arg1	consequence					256:266	the consequence	252:266	the consequence of a higher efficiency of AA use	252:299	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	8	48	theme	mammary	1890:1896	arg1	rate					1908:1911	mammary clearance rate	1890:1911	mammary clearance rate	1890:1911	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	3	49	theme	treatment	717:725	arg1	period					727:732	each treatment period	712:732	each treatment period	712:732	Blood samples were collected at the end of each treatment period from the mesenteric artery and mammary vein to determine mammary net nutrient fluxes.
26210277	6	50	theme	AA	1448:1449	arg1	efficiency					1417:1426	The conversion efficiency	1402:1426	The conversion efficiency of plasma essential AA into milk protein	1402:1467	The conversion efficiency of plasma essential AA into milk protein was improved with starch diets (33.7% on average) compared with fiber diets (27.5% on average).
26210277	1	51	theme	AA	294:295	arg1	use					297:299	AA use	294:299	AA use	294:299	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	10	52	theme	protein	2481:2487	arg1	yield					2489:2493	milk protein yield	2476:2493	milk protein yield	2476:2493	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	6	53	theme	plasma	1431:1436	arg1	AA					1448:1449	plasma essential AA	1431:1449	plasma essential AA into milk protein	1431:1467	The conversion efficiency of plasma essential AA into milk protein was improved with starch diets (33.7% on average) compared with fiber diets (27.5% on average).
26210277	4	54	theme	fiber	1073:1077	arg1	diets					1079:1083	fiber diets	1073:1083	fiber diets	1073:1083	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	5	55	theme	net	1141:1143	arg1	uptake					1145:1150	the mammary net uptake	1129:1150	the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level	1129:1399	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	3	56	theme	mesenteric	743:752	arg1	artery					754:759	mesenteric artery	743:759	mesenteric artery	743:759	Blood samples were collected at the end of each treatment period from the mesenteric artery and mammary vein to determine mammary net nutrient fluxes.
26210277	10	57	from	rich	2271:2274	arg1	fiber					2293:2297	fiber	2293:2297	fiber	2293:2297	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	10	57	from	rich	2271:2274	arg1	starch					2279:2284	starch	2279:2284	starch	2279:2284	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	2	58	theme	different	578:586	arg1	levels					596:601	2 different protein levels	576:601	2 different protein levels (12.0 vs. 16.5% CP)	576:621	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	2	58	theme	different	578:586	arg1	CP					619:620	12.0 vs. 16.5% CP	604:620	12.0 vs. 16.5% CP	604:620	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	7	59	theme	AA	1836:1837	arg1	metabolism					1839:1848	the mammary AA metabolism	1824:1848	the mammary AA metabolism	1824:1848	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	7	60	theme	efficiency	1585:1594	arg1	use					1596:1598	This higher mammary efficiency use	1565:1598	This higher mammary efficiency use of AA with starch diets	1565:1622	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	8	61	from	effect	1864:1869	arg1	uptake					1917:1922	uptake	1917:1922	uptake of BCAA observed in this study	1917:1953	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	8	61	from	effect	1864:1869	arg1	rate					1908:1911	mammary clearance rate	1890:1911	mammary clearance rate	1890:1911	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	10	62	from	starch	2279:2284	arg1	rich					2271:2274	rich	2271:2274	rich	2271:2274	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	5	63	theme	starch	1285:1290	arg1	diets					1305:1309	starch versus fiber diets	1285:1309	diets	1305:1309	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	7	64	theme	higher	1570:1575	arg1	use					1596:1598	This higher mammary efficiency use	1565:1598	This higher mammary efficiency use of AA with starch diets	1565:1622	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	8	65	theme	starch	1874:1879	arg1	diets					1881:1885	starch diets	1874:1885	starch diets	1874:1885	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	5	66	dep	total	1155:1159	arg1	%					1356:1356	7%	1355:1356	total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average)	1155:1368	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	10	67	from	fiber	2293:2297	arg1	rich					2271:2274	rich	2271:2274	rich	2271:2274	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	10	68	theme	mammary	2312:2318	arg1	utilization					2323:2333	the mammary AA utilization	2308:2333	the mammary AA utilization	2308:2333	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	5	69	theme	fiber	1299:1303	arg1	diets					1305:1309	starch versus fiber diets	1285:1309	diets	1305:1309	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	4	70	theme	normal	1099:1104	arg1	level					1109:1113	a normal CP level	1097:1113	a normal CP level	1097:1113	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	9	71	theme	higher	2107:2112	arg1	proportion					2114:2123	a higher proportion	2105:2123	a higher proportion of total essential AA in diets based on starch versus fiber	2105:2183	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	5	72	dep	uptake	1145:1150	arg1	addition					1119:1126	addition	1119:1126	addition	1119:1126	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	6	73	theme	starch	1487:1492	arg1	diets					1494:1498	starch diets	1487:1498	starch diets (33.7% on average)	1487:1517	The conversion efficiency of plasma essential AA into milk protein was improved with starch diets (33.7% on average) compared with fiber diets (27.5% on average).
26210277	7	74	theme	mammary	1802:1808	arg1	flow					1816:1819	the mammary blood flow	1798:1819	the mammary blood flow	1798:1819	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	5	75	theme	branched-chain	1177:1190	arg1	BCAA					1196:1199	BCAA	1196:1199	BCAA	1196:1199	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	5	75	theme	branched-chain	1177:1190	arg1	AA					1192:1193	branched-chain AA	1177:1193	branched-chain AA (BCAA)	1177:1200	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	8	76	theme	clearance	1898:1906	arg1	rate					1908:1911	mammary clearance rate	1890:1911	mammary clearance rate	1890:1911	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	3	77	theme	net	799:801	arg1	fluxes					812:817	mammary net nutrient fluxes	791:817	mammary net nutrient fluxes	791:817	Blood samples were collected at the end of each treatment period from the mesenteric artery and mammary vein to determine mammary net nutrient fluxes.
26210277	4	78	theme	mammary	915:921	arg1	uptake					927:932	mammary net uptake	915:932	mammary net uptake of acetate	915:943	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	2	79	theme	%	617:617	arg1	levels					596:601	2 different protein levels	576:601	2 different protein levels (12.0 vs. 16.5% CP)	576:621	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	2	79	theme	%	617:617	arg1	CP					619:620	12.0 vs. 16.5% CP	604:620	12.0 vs. 16.5% CP	604:620	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	9	80	theme	essential	2134:2142	arg1	AA					2144:2145	total essential AA	2128:2145	total essential AA in diets based on starch versus fiber	2128:2183	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	7	81	theme	CHO	1784:1786	arg1	effects					1773:1779	effects	1773:1779	effects of CHO on either the mammary blood flow or the mammary AA metabolism	1773:1848	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	6	82	from	%	1550:1550	arg1	average					1555:1561	average	1555:1561	average	1555:1561	The conversion efficiency of plasma essential AA into milk protein was improved with starch diets (33.7% on average) compared with fiber diets (27.5% on average).
26210277	9	83	from	proportion	2114:2123	arg1	diets					2150:2154	diets	2150:2154	diets based on starch versus fiber	2150:2183	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	10	84	theme	×	2354:2354	arg1	interactions					2359:2370	some CHO × CP interactions	2345:2370	some CHO × CP interactions on mammary metabolism	2345:2392	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	5	85	theme	milk	1335:1338	arg1	yield					1348:1352	a greater milk protein yield	1325:1352	a greater milk protein yield	1325:1352	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	4	86	theme	nutrients	834:842	arg1	nature					824:829	The nature	820:829	The nature of nutrients taken up by the mammary gland	820:872	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	6	87	dep	diets	1539:1543	arg1	%					1550:1550	27.5%	1546:1550	27.5%	1546:1550	The conversion efficiency of plasma essential AA into milk protein was improved with starch diets (33.7% on average) compared with fiber diets (27.5% on average).
26210277	1	88	theme	crude	370:374	arg1	protein					376:382	crude protein	370:382	dietary crude protein (CP) content	362:395	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	1	88	theme	crude	370:374	arg1	CP					385:386	CP	385:386	CP	385:386	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	7	89	theme	AA	1697:1698	arg1	extraction					1664:1673	a greater fractional extraction	1643:1673	a greater fractional extraction	1643:1673	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	7	89	theme	AA	1697:1698	arg1	rate					1689:1692	clearance rate	1679:1692	clearance rate	1679:1692	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	10	90	theme	mammary	2375:2381	arg1	metabolism					2383:2392	mammary metabolism	2375:2392	mammary metabolism	2375:2392	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	7	91	from	metabolism	1839:1848	arg1	absence					1762:1768	absence	1762:1768	absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism	1762:1848	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	1	92	theme	study	132:136	arg1	objective					114:122	The objective	110:122	The objective of this study	110:136	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	11	93	theme	glucose	2576:2582	arg1	metabolism					2584:2593	glucose metabolism	2576:2593	glucose metabolism	2576:2593	Results from this study suggest that mammary Leu and glucose metabolism can be modulated by the supply of glucogenic nutrients to the mammary gland.
26210277	8	94	located	observed	1932:1939	arg1	study					1949:1953	this study	1944:1953	this study	1944:1953	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	8	94	located	observed	1932:1939	arg2	BCAA					1927:1930	BCAA	1927:1930	BCAA observed in this study	1927:1953	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	10	95	theme	rich	2271:2274	arg1	Diets					2265:2269	Diets	2265:2269	Diets rich in starch versus fiber	2265:2297	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	2	96	theme	Latin	648:652	arg1	design					661:666	a 4 × 4 Latin square design	640:666	a 4 × 4 Latin square design	640:666	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	0	97	theme	Jersey	97:102	arg1	cows					104:107	lactating Jersey cows	87:107	lactating Jersey cows	87:107	Diets rich in starch improve the efficiency of amino acids use by the mammary gland in lactating Jersey cows.
26210277	7	98	theme	fractional	1653:1662	arg1	extraction					1664:1673	a greater fractional extraction	1643:1673	a greater fractional extraction	1643:1673	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	10	99	theme	CP	2512:2513	arg1	levels					2515:2520	the 2 studied CP levels	2498:2520	the 2 studied CP levels	2498:2520	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	6	100	theme	conversion	1406:1415	arg1	efficiency					1417:1426	The conversion efficiency	1402:1426	The conversion efficiency of plasma essential AA into milk protein	1402:1467	The conversion efficiency of plasma essential AA into milk protein was improved with starch diets (33.7% on average) compared with fiber diets (27.5% on average).
26210277	2	101	theme	×	644:644	arg1	design					661:666	a 4 × 4 Latin square design	640:666	a 4 × 4 Latin square design	640:666	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	4	102	theme	acetate	937:943	arg1	uptake					927:932	mammary net uptake	915:932	mammary net uptake of acetate	915:943	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	1	103	theme	greater	162:168	arg1	N					175:175	the greater milk N	158:175	the greater milk N	158:175	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	9	104	theme	milk	2213:2216	arg1	ratio					2225:2229	milk output ratio	2213:2229	milk output ratio	2213:2229	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	2	105	theme	isoenergetic	457:468	arg1	diets					470:474	4 isoenergetic diets	455:474	4 isoenergetic diets	455:474	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	9	106	theme	mammary	2195:2201	arg1	uptake					2203:2208	uptake	2203:2208	uptake	2203:2208	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	7	107	theme	mammary	1745:1751	arg1	gland					1753:1757	the mammary gland	1741:1757	the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism	1741:1848	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	9	108	dep	only	2065:2068	arg1	Pro					2259:2261	Pro	2259:2261	Pro	2259:2261	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	3	109	dep	artery	754:759	arg1	the					739:741	the	739:741	the	739:741	Blood samples were collected at the end of each treatment period from the mesenteric artery and mammary vein to determine mammary net nutrient fluxes.
26210277	11	110	from	study	2541:2545	arg1	Results					2523:2529	Results	2523:2529	Results from this study	2523:2545	Results from this study suggest that mammary Leu and glucose metabolism can be modulated by the supply of glucogenic nutrients to the mammary gland.
26210277	5	111	from	%	1244:1244	arg1	average					1249:1255	average	1249:1255	average	1249:1255	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	2	112	theme	carbohydrate	499:510	arg1	compositions					512:523	2 different carbohydrate compositions	487:523	2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP)	487:621	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	6	113	dep	diets	1494:1498	arg1	%					1505:1505	33.7%	1501:1505	33.7%	1501:1505	The conversion efficiency of plasma essential AA into milk protein was improved with starch diets (33.7% on average) compared with fiber diets (27.5% on average).
26210277	10	114	theme	CHO	2350:2352	arg1	interactions					2359:2370	some CHO × CP interactions	2345:2370	some CHO × CP interactions on mammary metabolism	2345:2392	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	10	115	theme	different	2417:2425	arg1	pathways					2437:2444	different metabolic pathways	2417:2444	different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels	2417:2520	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	10	116	theme	studied	2504:2510	arg1	levels					2515:2520	the 2 studied CP levels	2498:2520	the 2 studied CP levels	2498:2520	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	3	117	theme	Blood	669:673	arg1	samples					675:681	Blood samples	669:681	Blood samples	669:681	Blood samples were collected at the end of each treatment period from the mesenteric artery and mammary vein to determine mammary net nutrient fluxes.
26210277	0	118	theme	acids	53:57	arg1	efficiency					33:42	the efficiency	29:42	the efficiency of amino acids	29:57	Diets rich in starch improve the efficiency of amino acids use by the mammary gland in lactating Jersey cows.
26210277	10	119	theme	pathways	2437:2444	arg1	concept					2406:2412	the concept	2402:2412	the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels	2402:2520	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	9	120	theme	individual	2038:2047	arg1	AA					2049:2050	the individual AA	2034:2050	the individual AA	2034:2050	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	2	121	theme	multicatheterized	416:432	arg1	cows					441:444	Five midlactation multicatheterized Jersey cows	398:444	Five midlactation multicatheterized Jersey cows	398:444	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	0	122	from	starch	14:19	arg1	rich					6:9	rich	6:9	rich	6:9	Diets rich in starch improve the efficiency of amino acids use by the mammary gland in lactating Jersey cows.
26210277	8	123	theme	positive	1855:1862	arg1	effect					1864:1869	The positive effect	1851:1869	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study	1851:1953	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	4	124	theme	net	1003:1005	arg1	uptake					1007:1012	mammary net uptake	995:1012	mammary net uptake	995:1012	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	1	125	theme	higher	273:278	arg1	efficiency					280:289	a higher efficiency	271:289	a higher efficiency of AA use	271:299	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	7	126	theme	starch	1611:1616	arg1	diets					1618:1622	starch diets	1611:1622	starch diets	1611:1622	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	7	127	dep	group	1713:1717	arg1	Thr					1733:1735	Thr	1733:1735	Thr	1733:1735	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	7	127	dep	group	1713:1717	arg1	Lys					1728:1730	Lys	1728:1730	Lys	1728:1730	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	7	127	dep	group	1713:1717	arg1	BCAA					1722:1725	BCAA	1722:1725	BCAA	1722:1725	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	0	128	from	rich	6:9	arg1	starch					14:19	starch	14:19	starch	14:19	Diets rich in starch improve the efficiency of amino acids use by the mammary gland in lactating Jersey cows.
26210277	0	129	theme	mammary	70:76	arg1	gland					78:82	the mammary gland	66:82	the mammary gland in lactating Jersey cows	66:107	Diets rich in starch improve the efficiency of amino acids use by the mammary gland in lactating Jersey cows.
26210277	7	130	theme	AA	1603:1604	arg1	use					1596:1598	This higher mammary efficiency use	1565:1598	This higher mammary efficiency use of AA with starch diets	1565:1622	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	3	131	theme	period	727:732	arg1	end					705:707	the end	701:707	the end of each treatment period	701:732	Blood samples were collected at the end of each treatment period from the mesenteric artery and mammary vein to determine mammary net nutrient fluxes.
26210277	6	132	theme	essential	1438:1446	arg1	AA					1448:1449	plasma essential AA	1431:1449	plasma essential AA into milk protein	1431:1467	The conversion efficiency of plasma essential AA into milk protein was improved with starch diets (33.7% on average) compared with fiber diets (27.5% on average).
26210277	5	133	theme	mammary	1133:1139	arg1	uptake					1145:1150	the mammary net uptake	1129:1150	the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level	1129:1399	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	1	134	theme	use	297:299	arg1	efficiency					280:289	a higher efficiency	271:289	a higher efficiency of AA use	271:299	The objective of this study was to test whether the greater milk N yield usually observed when feeding diets based on starch versus fiber was the consequence of a higher efficiency of AA use across the mammary gland and whether this effect depended on dietary crude protein (CP) content.
26210277	3	135	theme	mammary	765:771	arg1	vein					773:776	mammary vein	765:776	mammary vein	765:776	Blood samples were collected at the end of each treatment period from the mesenteric artery and mammary vein to determine mammary net nutrient fluxes.
26210277	2	136	theme	protein	588:594	arg1	levels					596:601	2 different protein levels	576:601	2 different protein levels (12.0 vs. 16.5% CP)	576:621	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	2	136	theme	protein	588:594	arg1	CP					619:620	12.0 vs. 16.5% CP	604:620	12.0 vs. 16.5% CP	604:620	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	0	137	theme	rich	6:9	arg1	Diets					0:4	Diets	0:4	Diets rich in starch improve the efficiency of amino acids	0:57	Diets rich in starch improve the efficiency of amino acids use by the mammary gland in lactating Jersey cows.
26210277	7	138	theme	mammary	1577:1583	arg1	use					1596:1598	This higher mammary efficiency use	1565:1598	This higher mammary efficiency use of AA with starch diets	1565:1622	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	11	139	theme	mammary	2560:2566	arg1	Leu					2568:2570	mammary Leu	2560:2570	mammary Leu	2560:2570	Results from this study suggest that mammary Leu and glucose metabolism can be modulated by the supply of glucogenic nutrients to the mammary gland.
26210277	4	140	theme	mammary	860:866	arg1	gland					868:872	the mammary gland	856:872	the mammary gland	856:872	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	3	141	theme	mammary	791:797	arg1	fluxes					812:817	mammary net nutrient fluxes	791:817	mammary net nutrient fluxes	791:817	Blood samples were collected at the end of each treatment period from the mesenteric artery and mammary vein to determine mammary net nutrient fluxes.
26210277	2	142	from	rich	531:534	arg1	starch					539:544	starch	539:544	starch	539:544	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	4	143	theme	CP	1106:1107	arg1	level					1109:1113	a normal CP level	1097:1113	a normal CP level	1097:1113	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	7	144	theme	mammary	1828:1834	arg1	metabolism					1839:1848	the mammary AA metabolism	1824:1848	the mammary AA metabolism	1824:1848	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	10	145	theme	milk	2476:2479	arg1	yield					2489:2493	milk protein yield	2476:2493	milk protein yield	2476:2493	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	5	146	with	line	1315:1318	arg1	yield					1348:1352	a greater milk protein yield	1325:1352	a greater milk protein yield	1325:1352	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	2	147	theme	square	654:659	arg1	design					661:666	a 4 × 4 Latin square design	640:666	a 4 × 4 Latin square design	640:666	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	2	148	from	starch	539:544	arg1	rich					531:534	rich	531:534	rich	531:534	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	10	149	theme	CP	2356:2357	arg1	interactions					2359:2370	some CHO × CP interactions	2345:2370	some CHO × CP interactions on mammary metabolism	2345:2392	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	5	150	theme	CP	1392:1393	arg1	level					1395:1399	the CP level	1388:1399	the CP level	1388:1399	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	8	151	theme	diets	1881:1885	arg1	effect					1864:1869	The positive effect	1851:1869	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study	1851:1953	The positive effect of starch diets on mammary clearance rate and uptake of BCAA observed in this study was further improved when increasing dietary CP from 12.0 to 16.5%.
26210277	10	152	from	interactions	2359:2370	arg1	metabolism					2383:2392	mammary metabolism	2375:2392	mammary metabolism	2375:2392	Diets rich in starch versus fiber improved the mammary AA utilization; however, some CHO × CP interactions on mammary metabolism support the concept of different metabolic pathways by which starch diets improve milk protein yield at the 2 studied CP levels.
26210277	2	153	from	rich	550:553	arg1	fiber					558:562	fiber	558:562	fiber	558:562	Five midlactation multicatheterized Jersey cows were fed 4 isoenergetic diets to provide 2 different carbohydrate compositions (CHO; rich in starch vs. rich in fiber) crossed by 2 different protein levels (12.0 vs. 16.5% CP) and according to a 4 × 4 Latin square design.
26210277	7	154	theme	blood	1810:1814	arg1	flow					1816:1819	the mammary blood flow	1798:1819	the mammary blood flow	1798:1819	This higher mammary efficiency use of AA with starch diets was accompanied by a greater fractional extraction and clearance rate of AA belonging to group 2 (BCAA, Lys, Thr) by the mammary gland in absence of effects of CHO on either the mammary blood flow or the mammary AA metabolism.
26210277	9	155	theme	total	2128:2132	arg1	AA					2144:2145	total essential AA	2128:2145	total essential AA in diets based on starch versus fiber	2128:2183	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	4	156	theme	glucose	1036:1042	arg1	uptake					1007:1012	mammary net uptake	995:1012	mammary net uptake	995:1012	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	4	156	theme	glucose	1036:1042	arg1	rate					1028:1031	clearance rate	1018:1031	clearance rate of glucose	1018:1042	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	0	157	dep	starch	14:19	arg1	improve					21:27	improve	21:27	improve the efficiency of amino acids	21:57	Diets rich in starch improve the efficiency of amino acids use by the mammary gland in lactating Jersey cows.
26210277	3	158	theme	nutrient	803:810	arg1	fluxes					812:817	mammary net nutrient fluxes	791:817	mammary net nutrient fluxes	791:817	Blood samples were collected at the end of each treatment period from the mesenteric artery and mammary vein to determine mammary net nutrient fluxes.
26210277	5	159	theme	total	1155:1159	arg1	uptake					1145:1150	the mammary net uptake	1129:1150	the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level	1129:1399	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26210277	9	160	theme	AA	2144:2145	arg1	proportion					2114:2123	a higher proportion	2105:2123	a higher proportion of total essential AA in diets based on starch versus fiber	2105:2183	Concerning the individual AA, Leu was the only whose mammary uptake accounted for a higher proportion of total essential AA in diets based on starch versus fiber and whose mammary uptake to milk output ratio was modified (together with Pro).
26210277	4	161	theme	net	923:925	arg1	uptake					927:932	mammary net uptake	915:932	mammary net uptake of acetate	915:943	The nature of nutrients taken up by the mammary gland differed between starch and fiber diets: mammary net uptake of acetate increased with fiber versus starch diets, whereas mammary net uptake and clearance rate of glucose increased with starch versus fiber diets but only at a normal CP level.
26210277	6	162	theme	fiber	1533:1537	arg1	diets					1539:1543	fiber diets	1533:1543	fiber diets (27.5% on average)	1533:1562	The conversion efficiency of plasma essential AA into milk protein was improved with starch diets (33.7% on average) compared with fiber diets (27.5% on average).
26210277	5	163	theme	greater	1327:1333	arg1	yield					1348:1352	a greater milk protein yield	1325:1352	a greater milk protein yield	1325:1352	In addition, the mammary net uptake of total, essential, and branched-chain AA (BCAA) was significantly enhanced (12, 11, and 26% on average, respectively) when feeding starch versus fiber diets, in line with a greater milk protein yield (7% on average) and regardless of the CP level.
26820350	0	0	link	crosslinked	86:96	arg1	chitosan					98:105	crosslinked chitosan	86:105	crosslinked chitosan	86:105	Adsorptive removal of Congo red from aqueous solutions using crosslinked chitosan and crosslinked chitosan immobilized bentonite.
26820350	4	1	theme	CCS/BT	637:642	arg1	composite					644:652	CCS/BT composite	637:652	CCS/BT composite	637:652	The equilibrium data of CCS and CCS/BT composite agreed well with the Langmuir model.
26820350	6	2	theme	kinetic	815:821	arg1	data					823:826	The kinetic data	811:826	The kinetic data	811:826	The kinetic data correlated well with the pseudo-second-order model.
26820350	1	3	theme	crosslinked	240:250	arg1	chitosan					252:259	the crosslinked chitosan	236:259	the crosslinked chitosan (CCS)	236:265	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	1	3	theme	crosslinked	240:250	arg1	CCS					262:264	CCS	262:264	CCS	262:264	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	7	4	theme	electrostatic	1046:1058	arg1	attraction					1060:1069	the electrostatic attraction	1042:1069	the electrostatic attraction	1042:1069	The adsorption of CR onto the CCS was mainly controlled by chemisorption while the adsorption of CR onto the CCS/BT composite was controlled by chemisorption and the electrostatic attraction.
26820350	3	5	theme	CR	487:488	arg1	function					508:515	a function	506:515	a function of pH value of CR solution	506:542	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	5	theme	CR	487:488	arg1	time					553:556	contact time	545:556	contact time	545:556	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	5	theme	CR	487:488	arg1	salt					580:583	inorganic sodium salt	563:583	inorganic sodium salt	563:583	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	5	theme	CR	487:488	arg1	removal					476:482	The removal	472:482	The removal of CR	472:488	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	5	theme	CR	487:488	arg1	strength					595:602	ionic strength	589:602	ionic strength	589:602	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	7	6	theme	CR	898:899	arg1	adsorption					884:893	The adsorption	880:893	The adsorption of CR onto the CCS	880:912	The adsorption of CR onto the CCS was mainly controlled by chemisorption while the adsorption of CR onto the CCS/BT composite was controlled by chemisorption and the electrostatic attraction.
26820350	0	7	theme	crosslinked	86:96	arg1	chitosan					98:105	crosslinked chitosan	86:105	crosslinked chitosan	86:105	Adsorptive removal of Congo red from aqueous solutions using crosslinked chitosan and crosslinked chitosan immobilized bentonite.
26820350	0	8	from	solutions	45:53	arg1	removal					11:17	Adsorptive removal	0:17	Adsorptive removal of Congo red from aqueous solutions	0:53	Adsorptive removal of Congo red from aqueous solutions using crosslinked chitosan and crosslinked chitosan immobilized bentonite.
26820350	1	9	link	crosslinked	240:250	arg1	chitosan					252:259	the crosslinked chitosan	236:259	the crosslinked chitosan (CCS)	236:265	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	1	9	link	crosslinked	240:250	arg1	CCS					262:264	CCS	262:264	CCS	262:264	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	2	10	dep	Fourier	413:419	arg1	transform					421:429	transform	421:429	transform infrared spectroscopy	421:451	The CCS and CCS/BT composite were characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques.
26820350	2	11	theme	X-ray	385:389	arg1	XRD					404:406	XRD	404:406	XRD	404:406	The CCS and CCS/BT composite were characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques.
26820350	2	11	theme	X-ray	385:389	arg1	FTIR					454:457	FTIR	454:457	FTIR	454:457	The CCS and CCS/BT composite were characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques.
26820350	2	11	theme	X-ray	385:389	arg1	diffraction					391:401	X-ray diffraction	385:401	X-ray diffraction (XRD)	385:407	The CCS and CCS/BT composite were characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques.
26820350	0	12	theme	immobilized	107:117	arg1	bentonite					119:127	immobilized bentonite	107:127	immobilized bentonite	107:127	Adsorptive removal of Congo red from aqueous solutions using crosslinked chitosan and crosslinked chitosan immobilized bentonite.
26820350	2	13	theme	infrared	431:438	arg1	spectroscopy					440:451	infrared spectroscopy	431:451	infrared spectroscopy	431:451	The CCS and CCS/BT composite were characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques.
26820350	5	14	from	298K	748:751	arg1	mg/g					791:794	405 and 500 mg/g	779:794	405 and 500 mg/g	779:794	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	14	from	298K	748:751	arg1	CCS					720:722	CCS	720:722	CCS	720:722	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	14	from	298K	748:751	arg1	composite					735:743	CCS/BT composite	728:743	CCS/BT composite	728:743	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	14	from	298K	748:751	arg1	capacities					706:715	The adsorption capacities	691:715	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value	691:772	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	4	15	theme	Langmuir	675:682	arg1	model					684:688	the Langmuir model	671:688	the Langmuir model	671:688	The equilibrium data of CCS and CCS/BT composite agreed well with the Langmuir model.
26820350	0	16	theme	Adsorptive	0:9	arg1	removal					11:17	Adsorptive removal	0:17	Adsorptive removal of Congo red from aqueous solutions	0:53	Adsorptive removal of Congo red from aqueous solutions using crosslinked chitosan and crosslinked chitosan immobilized bentonite.
26820350	0	17	dep	chitosan	73:80	arg1	bentonite					119:127	immobilized bentonite	107:127	immobilized bentonite	107:127	Adsorptive removal of Congo red from aqueous solutions using crosslinked chitosan and crosslinked chitosan immobilized bentonite.
26820350	1	18	theme	red	198:200	arg1	removal					181:187	the removal	177:187	the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite)	177:331	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	2	19	theme	Fourier	413:419	arg1	techniques					460:469	X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques	385:469	X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques	385:469	The CCS and CCS/BT composite were characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques.
26820350	3	20	theme	pH	520:521	arg1	value					523:527	pH value	520:527	pH value of CR solution	520:542	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	5	21	theme	pH	765:766	arg1	value					768:772	natural pH value	757:772	natural pH value	757:772	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	3	22	theme	CR	532:533	arg1	solution					535:542	CR solution	532:542	CR solution	532:542	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	1	23	theme	crosslinked	271:281	arg1	bentonite					304:312	crosslinked chitosan immobilized bentonite	271:312	crosslinked chitosan immobilized bentonite (CCS/BT composite)	271:331	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	1	23	theme	crosslinked	271:281	arg1	composite					322:330	CCS/BT composite	315:330	CCS/BT composite	315:330	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	7	24	theme	CCS/BT	989:994	arg1	composite					996:1004	the CCS/BT composite	985:1004	the CCS/BT composite	985:1004	The adsorption of CR onto the CCS was mainly controlled by chemisorption while the adsorption of CR onto the CCS/BT composite was controlled by chemisorption and the electrostatic attraction.
26820350	3	25	theme	ionic	589:593	arg1	strength					595:602	ionic strength	589:602	ionic strength	589:602	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	25	theme	ionic	589:593	arg1	removal					476:482	The removal	472:482	The removal of CR	472:488	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	26	theme	value	523:527	arg1	function					508:515	a function	506:515	a function of pH value of CR solution	506:542	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	26	theme	value	523:527	arg1	time					553:556	contact time	545:556	contact time	545:556	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	26	theme	value	523:527	arg1	salt					580:583	inorganic sodium salt	563:583	inorganic sodium salt	563:583	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	26	theme	value	523:527	arg1	strength					595:602	ionic strength	589:602	ionic strength	589:602	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	26	theme	value	523:527	arg1	removal					476:482	The removal	472:482	The removal of CR	472:488	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	5	27	theme	CCS	720:722	arg1	mg/g					791:794	405 and 500 mg/g	779:794	405 and 500 mg/g	779:794	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	27	theme	CCS	720:722	arg1	capacities					706:715	The adsorption capacities	691:715	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value	691:772	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	1	28	theme	chitosan	283:290	arg1	bentonite					304:312	crosslinked chitosan immobilized bentonite	271:312	crosslinked chitosan immobilized bentonite (CCS/BT composite)	271:331	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	1	28	theme	chitosan	283:290	arg1	composite					322:330	CCS/BT composite	315:330	CCS/BT composite	315:330	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	7	29	theme	CR	977:978	arg1	adsorption					963:972	the adsorption	959:972	the adsorption of CR onto the CCS/BT composite	959:1004	The adsorption of CR onto the CCS was mainly controlled by chemisorption while the adsorption of CR onto the CCS/BT composite was controlled by chemisorption and the electrostatic attraction.
26820350	3	30	theme	contact	545:551	arg1	time					553:556	contact time	545:556	contact time	545:556	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	30	theme	contact	545:551	arg1	removal					476:482	The removal	472:482	The removal of CR	472:488	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	4	31	theme	equilibrium	609:619	arg1	data					621:624	The equilibrium data	605:624	The equilibrium data of CCS and CCS/BT composite	605:652	The equilibrium data of CCS and CCS/BT composite agreed well with the Langmuir model.
26820350	0	32	theme	red	28:30	arg1	removal					11:17	Adsorptive removal	0:17	Adsorptive removal of Congo red from aqueous solutions	0:53	Adsorptive removal of Congo red from aqueous solutions using crosslinked chitosan and crosslinked chitosan immobilized bentonite.
26820350	1	33	theme	immobilized	292:302	arg1	bentonite					304:312	crosslinked chitosan immobilized bentonite	271:312	crosslinked chitosan immobilized bentonite (CCS/BT composite)	271:331	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	1	33	theme	immobilized	292:302	arg1	composite					322:330	CCS/BT composite	315:330	CCS/BT composite	315:330	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	2	34	theme	diffraction	391:401	arg1	techniques					460:469	X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques	385:469	X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques	385:469	The CCS and CCS/BT composite were characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques.
26820350	5	35	theme	CCS/BT	728:733	arg1	composite					735:743	CCS/BT composite	728:743	CCS/BT composite	728:743	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	36	theme	composite	735:743	arg1	mg/g					791:794	405 and 500 mg/g	779:794	405 and 500 mg/g	779:794	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	36	theme	composite	735:743	arg1	capacities					706:715	The adsorption capacities	691:715	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value	691:772	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	3	37	theme	solution	535:542	arg1	value					523:527	pH value	520:527	pH value of CR solution	520:542	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	0	38	theme	aqueous	37:43	arg1	solutions					45:53	aqueous solutions	37:53	aqueous solutions	37:53	Adsorptive removal of Congo red from aqueous solutions using crosslinked chitosan and crosslinked chitosan immobilized bentonite.
26820350	6	39	theme	pseudo-second-order	853:871	arg1	model					873:877	the pseudo-second-order model	849:877	the pseudo-second-order model	849:877	The kinetic data correlated well with the pseudo-second-order model.
26820350	5	40	from	capacities	706:715	arg1	298K					748:751	298K	748:751	298K	748:751	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	40	from	capacities	706:715	arg1	value					768:772	natural pH value	757:772	natural pH value	757:772	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	0	41	link	crosslinked	61:71	arg1	chitosan					73:80	crosslinked chitosan	61:80	crosslinked chitosan	61:80	Adsorptive removal of Congo red from aqueous solutions using crosslinked chitosan and crosslinked chitosan immobilized bentonite.
26820350	1	42	theme	Batch	130:134	arg1	experiments					136:146	Batch experiments	130:146	Batch experiments	130:146	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	1	43	theme	CCS/BT	315:320	arg1	bentonite					304:312	crosslinked chitosan immobilized bentonite	271:312	crosslinked chitosan immobilized bentonite (CCS/BT composite)	271:331	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	1	43	theme	CCS/BT	315:320	arg1	composite					322:330	CCS/BT composite	315:330	CCS/BT composite	315:330	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	3	44	theme	inorganic	563:571	arg1	salt					580:583	inorganic sodium salt	563:583	inorganic sodium salt	563:583	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	44	theme	inorganic	563:571	arg1	removal					476:482	The removal	472:482	The removal of CR	472:488	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	5	45	from	value	768:772	arg1	mg/g					791:794	405 and 500 mg/g	779:794	405 and 500 mg/g	779:794	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	45	from	value	768:772	arg1	CCS					720:722	CCS	720:722	CCS	720:722	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	45	from	value	768:772	arg1	composite					735:743	CCS/BT composite	728:743	CCS/BT composite	728:743	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	45	from	value	768:772	arg1	capacities					706:715	The adsorption capacities	691:715	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value	691:772	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	1	46	theme	aqueous	212:218	arg1	solutions					220:228	aqueous solutions	212:228	aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite)	212:331	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	1	47	from	solutions	220:228	arg1	removal					181:187	the removal	177:187	the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite)	177:331	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	3	48	theme	sodium	573:578	arg1	salt					580:583	inorganic sodium salt	563:583	inorganic sodium salt	563:583	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	3	48	theme	sodium	573:578	arg1	removal					476:482	The removal	472:482	The removal of CR	472:488	The removal of CR was examined as a function of pH value of CR solution, contact time, and inorganic sodium salt and ionic strength.
26820350	1	49	link	crosslinked	271:281	arg1	bentonite					304:312	crosslinked chitosan immobilized bentonite	271:312	crosslinked chitosan immobilized bentonite (CCS/BT composite)	271:331	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	1	49	link	crosslinked	271:281	arg1	composite					322:330	CCS/BT composite	315:330	CCS/BT composite	315:330	Batch experiments were executed to investigate the removal of Congo red (CR) from aqueous solutions using the crosslinked chitosan (CCS) and crosslinked chitosan immobilized bentonite (CCS/BT composite).
26820350	0	50	theme	crosslinked	61:71	arg1	chitosan					73:80	crosslinked chitosan	61:80	crosslinked chitosan	61:80	Adsorptive removal of Congo red from aqueous solutions using crosslinked chitosan and crosslinked chitosan immobilized bentonite.
26820350	4	51	theme	CCS	629:631	arg1	data					621:624	The equilibrium data	605:624	The equilibrium data of CCS and CCS/BT composite	605:652	The equilibrium data of CCS and CCS/BT composite agreed well with the Langmuir model.
26820350	5	52	theme	adsorption	695:704	arg1	mg/g					791:794	405 and 500 mg/g	779:794	405 and 500 mg/g	779:794	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	52	theme	adsorption	695:704	arg1	capacities					706:715	The adsorption capacities	691:715	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value	691:772	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	5	53	theme	natural	757:763	arg1	value					768:772	natural pH value	757:772	natural pH value	757:772	The adsorption capacities of CCS and CCS/BT composite at 298K and natural pH value were 405 and 500 mg/g, respectively.
26820350	2	54	theme	CCS/BT	346:351	arg1	composite					353:361	CCS/BT composite	346:361	CCS/BT composite	346:361	The CCS and CCS/BT composite were characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) techniques.
26820350	4	55	theme	composite	644:652	arg1	data					621:624	The equilibrium data	605:624	The equilibrium data of CCS and CCS/BT composite	605:652	The equilibrium data of CCS and CCS/BT composite agreed well with the Langmuir model.
27155532	1	0	theme	order	301:305	arg1	effect					275:280	the effect	271:280	the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots	271:394	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	6	1	theme	respired	1097:1104	arg1	C					1106:1106	respired C	1097:1106	respired C	1097:1106	Species variation in δ (13) C of respired C may be partially explained by seasonal shifts from enriched to depleted C substrates in deciduous beech roots.
27155532	2	2	theme	stable	431:436	arg1	composition					447:457	the stable isotopic composition	427:457	the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots	427:545	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	0	3	theme	roots	124:128	arg1	dynamics					9:16	Seasonal dynamics	0:16	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.	0:129	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	7	4	theme	belowground	1414:1424	arg1	inquiry					1441:1447	belowground C-supply-chain inquiry	1414:1447	belowground C-supply-chain inquiry	1414:1447	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	4	5	theme	seasonal	854:861	arg1	trends					863:868	distinct seasonal trends	845:868	distinct seasonal trends in δ(13) C of respired C	845:893	Beech exhibited distinct seasonal trends in δ(13) C of respired C, while spruce did not.
27155532	6	6	theme	depleted	1171:1178	arg1	substrates					1182:1191	depleted C substrates	1171:1191	depleted C substrates	1171:1191	Species variation in δ (13) C of respired C may be partially explained by seasonal shifts from enriched to depleted C substrates in deciduous beech roots.
27155532	3	7	theme	organic	779:785	arg1	matter					787:792	bulk organic matter	774:792	bulk organic matter	774:792	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	1	8	theme	function	329:336	arg1	indicator					311:319	an indicator	308:319	an indicator of root function	308:336	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	6	9	theme	enriched	1159:1166	arg1	roots					1212:1216	enriched to depleted C substrates in deciduous beech roots	1159:1216	enriched to depleted C substrates in deciduous beech roots	1159:1216	Species variation in δ (13) C of respired C may be partially explained by seasonal shifts from enriched to depleted C substrates in deciduous beech roots.
27155532	3	10	located	observed	762:769	arg1	matter					787:792	bulk organic matter	774:792	bulk organic matter	774:792	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	3	10	located	observed	762:769	arg1	labile					795:800	labile	795:800	labile	795:800	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	3	10	located	observed	762:769	arg1	species					714:720	both species	709:720	both species	709:720	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	3	10	located	observed	762:769	arg2	differences					734:744	root order differences	723:744	root order differences in δ(13) C	723:755	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	3	10	located	observed	762:769	arg1	fractions					818:826	respired C fractions	807:826	respired C fractions	807:826	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	7	11	theme	C-supply-chain	1426:1439	arg1	inquiry					1441:1447	belowground C-supply-chain inquiry	1414:1447	belowground C-supply-chain inquiry	1414:1447	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	7	12	theme	C	1273:1273	arg1	composition					1284:1294	stable C isotopic composition	1266:1294	stable C isotopic composition of at least two root order groupings (first/second, third/fourth)	1266:1360	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	6	13	from	variation	1072:1080	arg1	C					1092:1092	δ (13) C	1085:1092	δ (13) C of respired C	1085:1106	Species variation in δ (13) C of respired C may be partially explained by seasonal shifts from enriched to depleted C substrates in deciduous beech roots.
27155532	2	14	dep	deciduous	498:506	arg1	sylvatica					515:523	Fagus sylvatica	509:523	Fagus sylvatica	509:523	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	4	15	theme	C	893:893	arg1	C					879:879	δ(13) C	873:879	δ(13) C of respired C	873:893	Beech exhibited distinct seasonal trends in δ(13) C of respired C, while spruce did not.
27155532	1	16	from	ratio	145:149	arg1	roots					160:164	plant roots	154:164	plant roots	154:164	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	1	17	dep	order	301:305	arg1	indicator					311:319	an indicator	308:319	an indicator of root function	308:336	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	7	18	theme	species	1233:1239	arg1	identity					1241:1248	species identity	1233:1248	species identity	1233:1248	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	1	19	from	roots	390:394	arg1	composition					355:365	the isotopic composition	342:365	the isotopic composition of C-rich fractions in roots	342:394	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	1	20	dep	function	202:209	arg1	storage					212:218	storage	212:218	storage	212:218	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	1	20	dep	function	202:209	arg1	uptake					227:232	uptake	227:232	uptake	227:232	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	5	21	theme	order	940:944	arg1	roots					952:956	first/second order beech roots	927:956	first/second order beech roots	927:956	In fall, first/second order beech roots were significantly depleted in (13) C, whereas spruce roots were enriched compared to higher order roots.
27155532	0	22	theme	fractions	39:47	arg1	C					27:27	δ(13) C	21:27	δ(13) C of C-rich fractions from Picea abies (Norway spruce)	21:80	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	0	23	theme	Picea	54:58	arg1	abies					60:64	Picea abies	54:64	Picea abies (Norway spruce)	54:80	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	0	23	theme	Picea	54:58	arg1	spruce					74:79	Norway spruce	67:79	Norway spruce	67:79	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	7	24	theme	groupings	1323:1331	arg1	composition					1284:1294	stable C isotopic composition	1266:1294	stable C isotopic composition of at least two root order groupings (first/second, third/fourth)	1266:1360	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	2	25	theme	Picea	477:481	arg1	evergreen					466:474	evergreen	466:474	evergreen	466:474	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	2	25	theme	Picea	477:481	arg1	abies					483:487	Picea abies	477:487	Picea abies	477:487	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	7	26	theme	root	1312:1315	arg1	groupings					1323:1331	at least two root order groupings	1299:1331	at least two root order groupings (first/second, third/fourth)	1299:1360	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	0	27	theme	Seasonal	0:7	arg1	dynamics					9:16	Seasonal dynamics	0:16	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.	0:129	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	3	28	theme	respired	807:814	arg1	fractions					818:826	respired C fractions	807:826	respired C fractions	807:826	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	2	29	theme	root	681:684	arg1	periods					697:703	root production periods	681:703	root production periods	681:703	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	1	30	theme	root	197:200	arg1	function					202:209	root function	197:209	root function (storage versus uptake)	197:233	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	1	31	from	composition	355:365	arg1	roots					390:394	roots	390:394	roots	390:394	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	3	32	theme	root	723:726	arg1	differences					734:744	root order differences	723:744	root order differences in δ(13) C	723:755	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	5	33	theme	order	1051:1055	arg1	roots					1057:1061	higher order roots	1044:1061	higher order roots	1044:1061	In fall, first/second order beech roots were significantly depleted in (13) C, whereas spruce roots were enriched compared to higher order roots.
27155532	6	34	theme	Species	1064:1070	arg1	variation					1072:1080	Species variation	1064:1080	Species variation in δ (13) C of respired C	1064:1106	Species variation in δ (13) C of respired C may be partially explained by seasonal shifts from enriched to depleted C substrates in deciduous beech roots.
27155532	2	35	theme	labile	590:595	arg1	C					597:597	respired and labile C	577:597	respired and labile C	577:597	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	1	36	theme	C	143:143	arg1	ratio					145:149	The (13/12) C ratio	131:149	The (13/12) C ratio in plant roots	131:164	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	1	36	theme	C	143:143	arg1	dynamic					176:182	dynamic	176:182	dynamic	176:182	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	3	37	theme	δ	749:749	arg1	C					755:755	δ(13) C	749:755	δ(13) C	749:755	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	2	38	theme	respired	577:584	arg1	C					597:597	respired and labile C	577:597	respired and labile C	577:597	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	4	39	theme	δ	873:873	arg1	C					879:879	δ(13) C	873:879	δ(13) C of respired C	873:893	Beech exhibited distinct seasonal trends in δ(13) C of respired C, while spruce did not.
27155532	1	40	theme	C-rich	370:375	arg1	fractions					377:385	C-rich fractions	370:385	C-rich fractions in roots	370:394	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	0	41	theme	fine	119:122	arg1	roots					124:128	δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots	21:128	δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots	21:128	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	6	42	theme	C	1106:1106	arg1	C					1092:1092	δ (13) C	1085:1092	δ (13) C of respired C	1085:1106	Species variation in δ (13) C of respired C may be partially explained by seasonal shifts from enriched to depleted C substrates in deciduous beech roots.
27155532	2	43	theme	isotopic	438:445	arg1	composition					447:457	the stable isotopic composition	427:457	the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots	427:545	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	5	44	dep	depleted	977:984	arg1	whereas					997:1003	whereas	997:1003	whereas	997:1003	In fall, first/second order beech roots were significantly depleted in (13) C, whereas spruce roots were enriched compared to higher order roots.
27155532	2	45	theme	order	646:650	arg1	roots					652:656	first/second and third/fourth order roots	616:656	first/second and third/fourth order roots	616:656	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	6	46	theme	C	1180:1180	arg1	substrates					1182:1191	depleted C substrates	1171:1191	depleted C substrates	1171:1191	Species variation in δ (13) C of respired C may be partially explained by seasonal shifts from enriched to depleted C substrates in deciduous beech roots.
27155532	2	47	theme	evergreen	466:474	arg1	composition					447:457	the stable isotopic composition	427:457	the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots	427:545	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	3	48	theme	bulk	774:777	arg1	matter					787:792	bulk organic matter	774:792	bulk organic matter	774:792	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	2	49	theme	δ	560:560	arg1	C					566:566	δ(13) C	560:566	δ(13) C of bulk	560:574	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	1	50	theme	root	324:327	arg1	function					329:336	root function	324:336	root function	324:336	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	4	51	from	trends	863:868	arg1	C					879:879	δ(13) C	873:879	δ(13) C of respired C	873:893	Beech exhibited distinct seasonal trends in δ(13) C of respired C, while spruce did not.
27155532	7	52	theme	isotopic	1275:1282	arg1	composition					1284:1294	stable C isotopic composition	1266:1294	stable C isotopic composition of at least two root order groupings (first/second, third/fourth)	1266:1360	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	2	53	theme	bulk	571:574	arg1	C					566:566	δ(13) C	560:566	δ(13) C of bulk	560:574	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	2	53	theme	bulk	571:574	arg1	C					597:597	respired and labile C	577:597	respired and labile C	577:597	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	2	53	theme	bulk	571:574	arg1	starch					604:609	starch	604:609	starch	604:609	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	7	54	theme	stable	1266:1271	arg1	composition					1284:1294	stable C isotopic composition	1266:1294	stable C isotopic composition of at least two root order groupings (first/second, third/fourth)	1266:1360	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	4	55	theme	distinct	845:852	arg1	trends					863:868	distinct seasonal trends	845:868	distinct seasonal trends in δ(13) C of respired C	845:893	Beech exhibited distinct seasonal trends in δ(13) C of respired C, while spruce did not.
27155532	0	56	theme	Fagus	86:90	arg1	roots					124:128	δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots	21:128	δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots	21:128	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	6	57	theme	seasonal	1138:1145	arg1	shifts					1147:1152	seasonal shifts	1138:1152	seasonal shifts from enriched to depleted C substrates in deciduous beech roots	1138:1216	Species variation in δ (13) C of respired C may be partially explained by seasonal shifts from enriched to depleted C substrates in deciduous beech roots.
27155532	4	58	theme	respired	884:891	arg1	C					893:893	respired C	884:893	respired C	884:893	Beech exhibited distinct seasonal trends in δ(13) C of respired C, while spruce did not.
27155532	7	59	dep	groupings	1323:1331	arg1	third/fourth					1348:1359	third/fourth	1348:1359	third/fourth	1348:1359	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	7	59	dep	groupings	1323:1331	arg1	first/second					1334:1345	first/second	1334:1345	first/second	1334:1345	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	1	60	theme	season	285:290	arg1	effect					275:280	the effect	271:280	the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots	271:394	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	0	61	from	abies	60:64	arg1	fractions					39:47	C-rich fractions	32:47	C-rich fractions from Picea abies (Norway spruce)	32:80	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	0	61	from	abies	60:64	arg1	C					27:27	δ(13) C	21:27	δ(13) C of C-rich fractions from Picea abies (Norway spruce)	21:80	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	0	62	theme	C-rich	32:37	arg1	fractions					39:47	C-rich fractions	32:47	C-rich fractions from Picea abies (Norway spruce)	32:80	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	1	63	theme	root	296:299	arg1	order					301:305	root order	296:305	root order (an indicator of root function)	296:337	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	6	64	theme	beech	1206:1210	arg1	roots					1212:1216	enriched to depleted C substrates in deciduous beech roots	1159:1216	enriched to depleted C substrates in deciduous beech roots	1159:1216	Species variation in δ (13) C of respired C may be partially explained by seasonal shifts from enriched to depleted C substrates in deciduous beech roots.
27155532	0	65	dep	Fagus	86:90	arg1	sylvatica					92:100	Fagus sylvatica	86:100	Fagus sylvatica (European beech)	86:117	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	5	66	theme	first/second	927:938	arg1	roots					952:956	first/second order beech roots	927:956	first/second order beech roots	927:956	In fall, first/second order beech roots were significantly depleted in (13) C, whereas spruce roots were enriched compared to higher order roots.
27155532	1	67	from	effect	275:280	arg1	composition					355:365	the isotopic composition	342:365	the isotopic composition of C-rich fractions in roots	342:394	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	6	68	from	roots	1212:1216	arg1	shifts					1147:1152	seasonal shifts	1138:1152	seasonal shifts from enriched to depleted C substrates in deciduous beech roots	1138:1216	Species variation in δ (13) C of respired C may be partially explained by seasonal shifts from enriched to depleted C substrates in deciduous beech roots.
27155532	5	69	theme	beech	946:950	arg1	roots					952:956	first/second order beech roots	927:956	first/second order beech roots	927:956	In fall, first/second order beech roots were significantly depleted in (13) C, whereas spruce roots were enriched compared to higher order roots.
27155532	7	70	from	differences	1251:1261	arg1	composition					1284:1294	stable C isotopic composition	1266:1294	stable C isotopic composition of at least two root order groupings (first/second, third/fourth)	1266:1360	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	0	71	from	C	27:27	arg1	abies					60:64	Picea abies	54:64	Picea abies (Norway spruce)	54:80	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	0	71	from	C	27:27	arg1	spruce					74:79	Norway spruce	67:79	Norway spruce	67:79	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	0	72	theme	Norway	67:72	arg1	abies					60:64	Picea abies	54:64	Picea abies (Norway spruce)	54:80	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	0	72	theme	Norway	67:72	arg1	spruce					74:79	Norway spruce	67:79	Norway spruce	67:79	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	3	73	theme	C	816:816	arg1	fractions					818:826	respired C fractions	807:826	respired C fractions	807:826	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	3	74	from	differences	734:744	arg1	C					755:755	δ(13) C	749:755	δ(13) C	749:755	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	5	75	theme	spruce	1005:1010	arg1	roots					1012:1016	spruce roots	1005:1016	spruce roots	1005:1016	In fall, first/second order beech roots were significantly depleted in (13) C, whereas spruce roots were enriched compared to higher order roots.
27155532	7	76	theme	order	1317:1321	arg1	groupings					1323:1331	at least two root order groupings	1299:1331	at least two root order groupings (first/second, third/fourth)	1299:1360	Regardless of species identity, differences in stable C isotopic composition of at least two root order groupings (first/second, third/fourth) were apparent, and should hereafter be separated in belowground C-supply-chain inquiry.
27155532	2	77	theme	production	686:695	arg1	periods					697:703	root production periods	681:703	root production periods	681:703	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	2	78	theme	deciduous	498:506	arg1	composition					447:457	the stable isotopic composition	427:457	the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots	427:545	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	6	79	theme	δ	1085:1085	arg1	C					1092:1092	δ (13) C	1085:1092	δ (13) C of respired C	1085:1106	Species variation in δ (13) C of respired C may be partially explained by seasonal shifts from enriched to depleted C substrates in deciduous beech roots.
27155532	1	80	from	fractions	377:385	arg1	roots					390:394	roots	390:394	roots	390:394	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	5	81	theme	higher	1044:1049	arg1	roots					1057:1061	higher order roots	1044:1061	higher order roots	1044:1061	In fall, first/second order beech roots were significantly depleted in (13) C, whereas spruce roots were enriched compared to higher order roots.
27155532	0	82	theme	C	27:27	arg1	roots					124:128	δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots	21:128	δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots	21:128	Seasonal dynamics of δ(13) C of C-rich fractions from Picea abies (Norway spruce) and Fagus sylvatica (European beech) fine roots.
27155532	3	83	theme	order	728:732	arg1	differences					734:744	root order differences	723:744	root order differences in δ(13) C	723:755	In both species, root order differences in δ(13) C were observed in bulk organic matter, labile, and respired C fractions.
27155532	1	84	theme	isotopic	346:353	arg1	composition					355:365	the isotopic composition	342:365	the isotopic composition of C-rich fractions in roots	342:394	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	2	85	theme	third/fourth	633:644	arg1	roots					652:656	first/second and third/fourth order roots	616:656	first/second and third/fourth order roots	616:656	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	2	86	theme	tree	527:530	arg1	evergreen					466:474	evergreen	466:474	evergreen	466:474	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	2	86	theme	tree	527:530	arg1	roots					541:545	tree species' roots	527:545	tree species' roots	527:545	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	2	87	theme	first/second	616:627	arg1	roots					652:656	first/second and third/fourth order roots	616:656	first/second and third/fourth order roots	616:656	To address this, we monitored the stable isotopic composition of one evergreen (Picea abies) and one deciduous (Fagus sylvatica), tree species' roots by measuring δ(13) C of bulk, respired and labile C, and starch from first/second and third/fourth order roots during spring and fall root production periods.
27155532	1	88	theme	fractions	377:385	arg1	composition					355:365	the isotopic composition	342:365	the isotopic composition of C-rich fractions in roots	342:394	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27155532	1	89	theme	plant	154:158	arg1	roots					160:164	plant roots	154:164	plant roots	154:164	The (13/12) C ratio in plant roots is likely dynamic depending on root function (storage versus uptake), but to date, little is known about the effect of season and root order (an indicator of root function) on the isotopic composition of C-rich fractions in roots.
27142164	9	0	theme	deduced	1350:1356	arg1	sequence					1369:1376	The deduced amino acid sequence	1346:1376	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA),	1346:1458	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	2	1	theme	anti-oxidant	349:360	arg1	activities					415:424	anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities	349:424	anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities	349:424	Ingestion of XOS has been reported to contribute to anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities.
27142164	11	2	theme	metabolic	1992:2000	arg1	performances					2002:2013	specific metabolic performances	1983:2013	specific metabolic performances	1983:2013	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	9	3	theme	cluster	1414:1420	arg1	genes					1392:1396	the genes	1388:1396	the genes of the xyl gene cluster	1388:1420	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	0	4	theme	rossiae	80:86	arg1	DSM					88:90	Lactobacillus rossiae DSM 15814	66:96	Lactobacillus rossiae DSM 15814(T)	66:99	Cloning, expression and characterization of a β-D-xylosidase from Lactobacillus rossiae DSM 15814(T).
27142164	0	4	theme	rossiae	80:86	arg1	T					98:98	T	98:98	T	98:98	Cloning, expression and characterization of a β-D-xylosidase from Lactobacillus rossiae DSM 15814(T).
27142164	5	5	theme	industrial	785:794	arg1	scale					796:800	an industrial scale	782:800	an industrial scale for biotechnological purposes	782:830	Xylanase cocktails are used on an industrial scale for biotechnological purposes.
27142164	3	6	dep	complexity	453:462	arg1	the					438:440	the	438:440	the	438:440	Because of the structural complexity and chemical heterogeneity, complete degradation of xylan-containing plant polymers requires the synergistic activity of several enzymes.
27142164	3	7	theme	several	585:591	arg1	enzymes					593:599	several enzymes	585:599	several enzymes	585:599	Because of the structural complexity and chemical heterogeneity, complete degradation of xylan-containing plant polymers requires the synergistic activity of several enzymes.
27142164	10	8	theme	β-D-xylosidase	1739:1752	arg1	activity					1754:1761	β-D-xylosidase activity	1739:1761	β-D-xylosidase activity of L. rossiae DSM 15814(T)	1739:1788	Heterologously expressed XylA was shown to completely hydrolyse XOS to xylose and showed optimal activity at pH 6.0 and 40 °C. Furthermore, β-D-xylosidase activity of L. rossiae DSM 15814(T) was also measured under sourdough conditions.
27142164	11	9	theme	L.	1885:1886	arg1	DSM					1896:1898	L. rossiae DSM 15814	1885:1904	L. rossiae DSM 15814(T)	1885:1907	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	11	9	theme	L.	1885:1886	arg1	T					1906:1906	T	1906:1906	T	1906:1906	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	8	10	theme	designated	1178:1187	arg1	clusters					1168:1175	two gene clusters	1159:1175	two gene clusters	1159:1175	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	8	10	theme	designated	1178:1187	arg1	xyl					1189:1191	designated xyl	1178:1191	designated xyl	1178:1191	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	9	11	theme	amino	1358:1362	arg1	sequence					1369:1376	The deduced amino acid sequence	1346:1376	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA),	1346:1458	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	3	12	theme	enzymes	593:599	arg1	activity					573:580	the synergistic activity	557:580	the synergistic activity of several enzymes	557:599	Because of the structural complexity and chemical heterogeneity, complete degradation of xylan-containing plant polymers requires the synergistic activity of several enzymes.
27142164	4	13	theme	termed	651:656	arg1	Endo-xylanases					602:615	Endo-xylanases	602:615	Endo-xylanases	602:615	Endo-xylanases and β-D-xylosidases, collectively termed xylanases, represent the two key enzymes responsible for the sequential hydrolysis of xylan.
27142164	4	13	theme	termed	651:656	arg1	xylanases					658:666	collectively termed xylanases	638:666	collectively termed xylanases	638:666	Endo-xylanases and β-D-xylosidases, collectively termed xylanases, represent the two key enzymes responsible for the sequential hydrolysis of xylan.
27142164	8	14	theme	L-arabinose	1308:1318	arg1	metabolism					1320:1329	L-arabinose metabolism	1308:1329	L-arabinose metabolism	1308:1329	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	4	15	theme	key	687:689	arg1	enzymes					691:697	the two key enzymes	679:697	the two key enzymes responsible for the sequential hydrolysis of xylan	679:748	Endo-xylanases and β-D-xylosidases, collectively termed xylanases, represent the two key enzymes responsible for the sequential hydrolysis of xylan.
27142164	9	16	theme	acid	1539:1542	arg1	bacteria					1544:1551	various lactic acid bacteria	1524:1551	various lactic acid bacteria	1524:1551	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	1	17	theme	gut	171:173	arg1	microbiota					175:184	the gut microbiota	167:184	the gut microbiota	167:184	BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties.
27142164	2	18	theme	anti-diabetic	401:413	arg1	activities					415:424	anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities	349:424	anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities	349:424	Ingestion of XOS has been reported to contribute to anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities.
27142164	5	19	theme	biotechnological	806:821	arg1	purposes					823:830	biotechnological purposes	806:830	biotechnological purposes	806:830	Xylanase cocktails are used on an industrial scale for biotechnological purposes.
27142164	11	20	theme	rossiae	1888:1894	arg1	DSM					1896:1898	L. rossiae DSM 15814	1885:1904	L. rossiae DSM 15814(T)	1885:1907	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	11	20	theme	rossiae	1888:1894	arg1	T					1906:1906	T	1906:1906	T	1906:1906	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	8	21	theme	XOS	1252:1254	arg1	uptake					1256:1261	XOS uptake	1252:1261	XOS uptake	1252:1261	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	8	22	theme	clusters	1168:1175	arg1	presence					1147:1154	the presence	1143:1154	the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively	1143:1343	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	8	23	theme	gene	1163:1166	arg1	clusters					1168:1175	two gene clusters	1159:1175	two gene clusters	1159:1175	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	8	23	theme	gene	1163:1166	arg1	xyl					1189:1191	designated xyl	1178:1191	designated xyl	1178:1191	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	8	23	theme	gene	1163:1166	arg1	ara					1197:1199	ara	1197:1199	ara	1197:1199	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	10	24	theme	L.	1766:1767	arg1	DSM					1777:1779	L. rossiae DSM 15814	1766:1785	L. rossiae DSM 15814(T)	1766:1788	Heterologously expressed XylA was shown to completely hydrolyse XOS to xylose and showed optimal activity at pH 6.0 and 40 °C. Furthermore, β-D-xylosidase activity of L. rossiae DSM 15814(T) was also measured under sourdough conditions.
27142164	10	24	theme	L.	1766:1767	arg1	T					1787:1787	T	1787:1787	T	1787:1787	Heterologously expressed XylA was shown to completely hydrolyse XOS to xylose and showed optimal activity at pH 6.0 and 40 °C. Furthermore, β-D-xylosidase activity of L. rossiae DSM 15814(T) was also measured under sourdough conditions.
27142164	2	25	theme	immune-modulatory	379:395	arg1	activities					415:424	anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities	349:424	anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities	349:424	Ingestion of XOS has been reported to contribute to anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities.
27142164	9	26	theme	acid	1364:1367	arg1	sequence					1369:1376	The deduced amino acid sequence	1346:1376	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA),	1346:1458	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	1	27	theme	promising	264:272	arg1	properties					285:294	promising functional properties	264:294	promising functional properties	264:294	BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties.
27142164	11	28	theme	useful	1941:1946	arg1	XOS					1920:1922	XOS	1920:1922	XOS	1920:1922	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	11	28	theme	useful	1941:1946	arg1	trait					1948:1952	a very useful trait	1934:1952	a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics	1934:2057	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	2	29	theme	XOS	310:312	arg1	Ingestion					297:305	Ingestion	297:305	Ingestion of XOS	297:312	Ingestion of XOS has been reported to contribute to anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities.
27142164	3	30	theme	structural	442:451	arg1	complexity					453:462	structural complexity	442:462	structural complexity	442:462	Because of the structural complexity and chemical heterogeneity, complete degradation of xylan-containing plant polymers requires the synergistic activity of several enzymes.
27142164	1	31	theme	functional	274:283	arg1	properties					285:294	promising functional properties	264:294	promising functional properties	264:294	BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties.
27142164	11	32	theme	specific	1983:1990	arg1	performances					2002:2013	specific metabolic performances	1983:2013	specific metabolic performances	1983:2013	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	6	33	theme	DSM	855:857	arg1	T					865:865	T	865:865	T	865:865	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	6	33	theme	DSM	855:857	arg1	15814					859:863	Lactobacillus rossiae DSM 15814	833:863	Lactobacillus rossiae DSM 15814(T)	833:866	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	10	34	theme	DSM	1777:1779	arg1	activity					1754:1761	β-D-xylosidase activity	1739:1761	β-D-xylosidase activity of L. rossiae DSM 15814(T)	1739:1788	Heterologously expressed XylA was shown to completely hydrolyse XOS to xylose and showed optimal activity at pH 6.0 and 40 °C. Furthermore, β-D-xylosidase activity of L. rossiae DSM 15814(T) was also measured under sourdough conditions.
27142164	8	35	theme	D-xylose	1282:1289	arg1	utilization					1291:1301	D-xylose utilization	1282:1301	D-xylose utilization	1282:1301	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	11	36	with	starters	1969:1976	arg1	performances					2002:2013	specific metabolic performances	1983:2013	specific metabolic performances	1983:2013	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	8	37	theme	Genomic	1098:1104	arg1	analyses					1125:1132	RESULTS Genomic and transcriptomic analyses	1090:1132	RESULTS Genomic and transcriptomic analyses	1090:1132	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	0	38	from	DSM	88:90	arg1	expression					9:18	expression	9:18	expression	9:18	Cloning, expression and characterization of a β-D-xylosidase from Lactobacillus rossiae DSM 15814(T).
27142164	0	38	from	DSM	88:90	arg1	Cloning					0:6	Cloning	0:6	Cloning	0:6	Cloning, expression and characterization of a β-D-xylosidase from Lactobacillus rossiae DSM 15814(T).
27142164	0	38	from	DSM	88:90	arg1	characterization					24:39	characterization	24:39	characterization	24:39	Cloning, expression and characterization of a β-D-xylosidase from Lactobacillus rossiae DSM 15814(T).
27142164	9	39	dep	β-D-xylosidases	1497:1511	arg1	predicted					1486:1494	predicted	1486:1494	predicted	1486:1494	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	9	40	dep	LROS_1108	1423:1431	arg1	designated					1434:1443	designated	1434:1443	designated here as xylA	1434:1456	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	3	41	theme	complete	492:499	arg1	degradation					501:511	complete degradation	492:511	complete degradation of xylan-containing plant polymers	492:546	Because of the structural complexity and chemical heterogeneity, complete degradation of xylan-containing plant polymers requires the synergistic activity of several enzymes.
27142164	3	42	theme	polymers	539:546	arg1	degradation					501:511	complete degradation	492:511	complete degradation of xylan-containing plant polymers	492:546	Because of the structural complexity and chemical heterogeneity, complete degradation of xylan-containing plant polymers requires the synergistic activity of several enzymes.
27142164	10	43	theme	optimal	1688:1694	arg1	activity					1696:1703	optimal activity	1688:1703	optimal activity	1688:1703	Heterologously expressed XylA was shown to completely hydrolyse XOS to xylose and showed optimal activity at pH 6.0 and 40 °C. Furthermore, β-D-xylosidase activity of L. rossiae DSM 15814(T) was also measured under sourdough conditions.
27142164	9	44	theme	xyl	1405:1407	arg1	cluster					1414:1420	the xyl gene cluster	1401:1420	the xyl gene cluster	1401:1420	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	9	45	theme	glycosyl	1569:1576	arg1	family					1588:1593	glycosyl hydrolase family 43	1569:1596	glycosyl hydrolase family 43	1569:1596	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	3	46	theme	chemical	468:475	arg1	heterogeneity					477:489	chemical heterogeneity	468:489	chemical heterogeneity	468:489	Because of the structural complexity and chemical heterogeneity, complete degradation of xylan-containing plant polymers requires the synergistic activity of several enzymes.
27142164	4	47	theme	sequential	719:728	arg1	hydrolysis					730:739	the sequential hydrolysis	715:739	the sequential hydrolysis of xylan	715:748	Endo-xylanases and β-D-xylosidases, collectively termed xylanases, represent the two key enzymes responsible for the sequential hydrolysis of xylan.
27142164	9	48	theme	one	1381:1383	arg1	sequence					1369:1376	The deduced amino acid sequence	1346:1376	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA),	1346:1458	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	8	49	theme	transcriptomic	1110:1123	arg1	analyses					1125:1132	RESULTS Genomic and transcriptomic analyses	1090:1132	RESULTS Genomic and transcriptomic analyses	1090:1132	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	9	50	theme	high	1466:1469	arg1	similarity					1471:1480	high similarity	1466:1480	high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria	1466:1551	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	9	51	theme	hydrolase	1578:1586	arg1	family					1588:1593	glycosyl hydrolase family 43	1569:1596	glycosyl hydrolase family 43	1569:1596	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	6	52	theme	rossiae	847:853	arg1	T					865:865	T	865:865	T	865:865	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	6	52	theme	rossiae	847:853	arg1	15814					859:863	Lactobacillus rossiae DSM 15814	833:863	Lactobacillus rossiae DSM 15814(T)	833:866	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	4	53	theme	responsible	699:709	arg1	enzymes					691:697	the two key enzymes	679:697	the two key enzymes responsible for the sequential hydrolysis of xylan	679:748	Endo-xylanases and β-D-xylosidases, collectively termed xylanases, represent the two key enzymes responsible for the sequential hydrolysis of xylan.
27142164	0	54	theme	β-D-xylosidase	46:59	arg1	expression					9:18	expression	9:18	expression	9:18	Cloning, expression and characterization of a β-D-xylosidase from Lactobacillus rossiae DSM 15814(T).
27142164	0	54	theme	β-D-xylosidase	46:59	arg1	Cloning					0:6	Cloning	0:6	Cloning	0:6	Cloning, expression and characterization of a β-D-xylosidase from Lactobacillus rossiae DSM 15814(T).
27142164	0	54	theme	β-D-xylosidase	46:59	arg1	characterization					24:39	characterization	24:39	characterization	24:39	Cloning, expression and characterization of a β-D-xylosidase from Lactobacillus rossiae DSM 15814(T).
27142164	6	55	theme	sources	907:913	arg1	likely					935:940	likely	935:940	likely	935:940	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	6	55	theme	sources	907:913	arg1	set					893:895	an extensive set	880:895	an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability	880:978	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	6	55	theme	sources	907:913	arg1	sources					907:913	carbon sources	900:913	carbon sources	900:913	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	6	55	theme	sources	907:913	arg1	ability					919:925	an ability	916:925	an ability that is likely to contribute to its adaptive ability	916:978	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	1	56	dep	affect	160:165	arg1	possess					256:262	possess	256:262	possess promising functional properties	256:294	BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties.
27142164	6	57	theme	Lactobacillus	833:845	arg1	T					865:865	T	865:865	T	865:865	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	6	57	theme	Lactobacillus	833:845	arg1	15814					859:863	Lactobacillus rossiae DSM 15814	833:863	Lactobacillus rossiae DSM 15814(T)	833:866	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	3	58	theme	xylan-containing	516:531	arg1	polymers					539:546	xylan-containing plant polymers	516:546	xylan-containing plant polymers	516:546	Because of the structural complexity and chemical heterogeneity, complete degradation of xylan-containing plant polymers requires the synergistic activity of several enzymes.
27142164	8	59	dep	Genomic	1098:1104	arg1	RESULTS					1090:1096	RESULTS	1090:1096	RESULTS	1090:1096	RESULTS Genomic and transcriptomic analyses revealed the presence of two gene clusters, designated xyl and ara, encoding proteins predicted to be responsible for XOS uptake and hydrolysis and D-xylose utilization, and L-arabinose metabolism, respectively.
27142164	10	60	theme	sourdough	1814:1822	arg1	conditions					1824:1833	sourdough conditions	1814:1833	sourdough conditions	1814:1833	Heterologously expressed XylA was shown to completely hydrolyse XOS to xylose and showed optimal activity at pH 6.0 and 40 °C. Furthermore, β-D-xylosidase activity of L. rossiae DSM 15814(T) was also measured under sourdough conditions.
27142164	6	61	theme	carbon	900:905	arg1	likely					935:940	likely	935:940	likely	935:940	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	6	61	theme	carbon	900:905	arg1	sources					907:913	carbon sources	900:913	carbon sources	900:913	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	6	61	theme	carbon	900:905	arg1	ability					919:925	an ability	916:925	an ability that is likely to contribute to its adaptive ability	916:978	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	10	62	theme	rossiae	1769:1775	arg1	DSM					1777:1779	L. rossiae DSM 15814	1766:1785	L. rossiae DSM 15814(T)	1766:1788	Heterologously expressed XylA was shown to completely hydrolyse XOS to xylose and showed optimal activity at pH 6.0 and 40 °C. Furthermore, β-D-xylosidase activity of L. rossiae DSM 15814(T) was also measured under sourdough conditions.
27142164	10	62	theme	rossiae	1769:1775	arg1	T					1787:1787	T	1787:1787	T	1787:1787	Heterologously expressed XylA was shown to completely hydrolyse XOS to xylose and showed optimal activity at pH 6.0 and 40 °C. Furthermore, β-D-xylosidase activity of L. rossiae DSM 15814(T) was also measured under sourdough conditions.
27142164	3	63	theme	plant	533:537	arg1	polymers					539:546	xylan-containing plant polymers	516:546	xylan-containing plant polymers	516:546	Because of the structural complexity and chemical heterogeneity, complete degradation of xylan-containing plant polymers requires the synergistic activity of several enzymes.
27142164	9	64	theme	lactic	1532:1537	arg1	bacteria					1544:1551	various lactic acid bacteria	1524:1551	various lactic acid bacteria	1524:1551	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	9	65	theme	genes	1392:1396	arg1	LROS_1108					1423:1431	LROS_1108	1423:1431	LROS_1108	1423:1431	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	9	65	theme	genes	1392:1396	arg1	one					1381:1383	one	1381:1383	one	1381:1383	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	9	65	theme	genes	1392:1396	arg1	genes					1392:1396	the genes	1388:1396	the genes of the xyl gene cluster	1388:1420	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	5	66	theme	Xylanase	751:758	arg1	cocktails					760:768	Xylanase cocktails	751:768	Xylanase cocktails	751:768	Xylanase cocktails are used on an industrial scale for biotechnological purposes.
27142164	1	67	contain	possess	256:262	arg1	xylo-oligosaccharides					187:207	xylo-oligosaccharides	187:207	xylo-oligosaccharides (XOS)	187:213	BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties.
27142164	1	67	contain	possess	256:262	arg1	XOS					210:212	XOS	210:212	XOS	210:212	BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties.
27142164	1	67	contain	possess	256:262	arg1	oligosaccharides					232:247	arabinoxylan oligosaccharides	219:247	arabinoxylan oligosaccharides (AXOS)	219:254	BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties.
27142164	1	67	contain	possess	256:262	arg1	AXOS					250:253	AXOS	250:253	AXOS	250:253	BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties.
27142164	1	67	contain	possess	256:262	arg2	properties					285:294	promising functional properties	264:294	promising functional properties	264:294	BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties.
27142164	11	68	dep	CONCLUSIONS	1836:1846	arg1	highlights					1859:1868	highlights	1859:1868	highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics	1859:2057	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	11	69	theme	sourdough	2019:2027	arg1	fermentation					2029:2040	sourdough fermentation	2019:2040	sourdough fermentation	2019:2040	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	9	70	theme	various	1524:1530	arg1	bacteria					1544:1551	various lactic acid bacteria	1524:1551	various lactic acid bacteria	1524:1551	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
27142164	7	71	theme	strain	1017:1022	arg1	capacity					1000:1007	the capacity	996:1007	the capacity of this strain to utilize XOS, xylan, D-xylose and L-arabinose	996:1070	In this study, the capacity of this strain to utilize XOS, xylan, D-xylose and L-arabinose was investigated.
27142164	4	72	theme	xylan	744:748	arg1	hydrolysis					730:739	the sequential hydrolysis	715:739	the sequential hydrolysis of xylan	715:748	Endo-xylanases and β-D-xylosidases, collectively termed xylanases, represent the two key enzymes responsible for the sequential hydrolysis of xylan.
27142164	0	73	theme	Lactobacillus	66:78	arg1	DSM					88:90	Lactobacillus rossiae DSM 15814	66:96	Lactobacillus rossiae DSM 15814(T)	66:99	Cloning, expression and characterization of a β-D-xylosidase from Lactobacillus rossiae DSM 15814(T).
27142164	0	73	theme	Lactobacillus	66:78	arg1	T					98:98	T	98:98	T	98:98	Cloning, expression and characterization of a β-D-xylosidase from Lactobacillus rossiae DSM 15814(T).
27142164	6	74	theme	extensive	883:891	arg1	likely					935:940	likely	935:940	likely	935:940	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	6	74	theme	extensive	883:891	arg1	set					893:895	an extensive set	880:895	an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability	880:978	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	6	74	theme	extensive	883:891	arg1	sources					907:913	carbon sources	900:913	carbon sources	900:913	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	6	74	theme	extensive	883:891	arg1	ability					919:925	an ability	916:925	an ability that is likely to contribute to its adaptive ability	916:978	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	11	75	theme	DSM	1896:1898	arg1	ability					1874:1880	the ability	1870:1880	the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics	1870:2057	CONCLUSIONS This study highlights the ability of L. rossiae DSM 15814(T) to utilize XOS, which is a very useful trait when selecting starters with specific metabolic performances for sourdough fermentation or as probiotics.
27142164	2	76	theme	anti-bacterial	363:376	arg1	activities					415:424	anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities	349:424	anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities	349:424	Ingestion of XOS has been reported to contribute to anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities.
27142164	1	77	theme	arabinoxylan	219:230	arg1	AXOS					250:253	AXOS	250:253	AXOS	250:253	BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties.
27142164	1	77	theme	arabinoxylan	219:230	arg1	oligosaccharides					232:247	arabinoxylan oligosaccharides	219:247	arabinoxylan oligosaccharides (AXOS)	219:254	BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties.
27142164	5	78	used	used	774:777	arg2	cocktails					760:768	Xylanase cocktails	751:768	Xylanase cocktails	751:768	Xylanase cocktails are used on an industrial scale for biotechnological purposes.
27142164	6	79	theme	adaptive	963:970	arg1	ability					972:978	its adaptive ability	959:978	its adaptive ability	959:978	Lactobacillus rossiae DSM 15814(T) can utilize an extensive set of carbon sources, an ability that is likely to contribute to its adaptive ability.
27142164	3	80	theme	synergistic	561:571	arg1	activity					573:580	the synergistic activity	557:580	the synergistic activity of several enzymes	557:599	Because of the structural complexity and chemical heterogeneity, complete degradation of xylan-containing plant polymers requires the synergistic activity of several enzymes.
27142164	10	81	theme	expressed	1614:1622	arg1	XylA					1624:1627	Heterologously expressed XylA	1599:1627	Heterologously expressed XylA	1599:1627	Heterologously expressed XylA was shown to completely hydrolyse XOS to xylose and showed optimal activity at pH 6.0 and 40 °C. Furthermore, β-D-xylosidase activity of L. rossiae DSM 15814(T) was also measured under sourdough conditions.
27142164	9	82	theme	gene	1409:1412	arg1	cluster					1414:1420	the xyl gene cluster	1401:1420	the xyl gene cluster	1401:1420	The deduced amino acid sequence of one of the genes of the xyl gene cluster, LROS_1108 (designated here as xylA), shows high similarity to (predicted) β-D-xylosidases encoded by various lactic acid bacteria, and belongs to glycosyl hydrolase family 43.
29326706	4	0	theme	IL-12	645:649	arg1	production					603:612	production	603:612	production	603:612	In vitro, these interactions trigger cell signaling that leads to NF-κB activation and production of the Th1 polarizing cytokine IL-12.
29326706	4	0	theme	IL-12	645:649	arg1	activation					588:597	activation	588:597	activation	588:597	In vitro, these interactions trigger cell signaling that leads to NF-κB activation and production of the Th1 polarizing cytokine IL-12.
29326706	1	1	theme	toll-like	134:142	arg1	TLRs					155:158	TLRs	155:158	TLRs	155:158	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	1	1	theme	toll-like	134:142	arg1	receptors					144:152	toll-like receptors	134:152	toll-like receptors (TLRs)	134:159	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	9	2	theme	other	1329:1333	arg1	hand					1335:1338	the other hand	1325:1338	the other hand	1325:1338	On the other hand, we showed that TLR-mediated cell activation might be triggered by an alternative way, in which lectins bind to TLRs N-glycans and stimulate cells to increase the expression of pro-inflammatory cytokines.
29326706	6	3	theme	pathogens	847:855	arg1	lectins					826:832	The lectins	822:832	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin)	822:940	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	3	4	theme	different	466:474	arg1	structures					476:485	different structures	466:485	different structures	466:485	Expression of the receptors on the surface of antigen-presenting cells exposes N-glycans that may be targeted by lectins of different structures, specificities, and origins.
29326706	6	5	theme	in	1014:1015	arg1	effects					1022:1028	in vivo effects	1014:1028	in vivo effects	1014:1028	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	6	6	theme	human	841:845	arg1	pathogens					847:855	the human pathogens	837:855	the human pathogens	837:855	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	10	7	theme	intracellular	1672:1684	arg1	pathogens					1686:1694	intracellular pathogens	1672:1694	intracellular pathogens	1672:1694	This process may lead to the development of new pharmaceutical tools that promote protective immune responses directed against intracellular pathogens and tumors.
29326706	1	8	theme	receptors	144:152	arg1	properties					187:196	immunomodulatory properties	170:196	immunomodulatory properties	170:196	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	1	8	theme	receptors	144:152	arg1	lectins					107:113	some lectins	102:113	some lectins	102:113	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	1	8	theme	receptors	144:152	arg1	agonists					122:129	agonists	122:129	agonists of toll-like receptors (TLRs)	122:159	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	6	9	theme	lectin	1070:1075	arg1	ArtinM					1077:1082	the plant lectin ArtinM	1060:1082	the plant lectin ArtinM	1060:1082	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	3	10	theme	origins	507:513	arg1	lectins					455:461	lectins	455:461	lectins of different structures, specificities, and origins	455:513	Expression of the receptors on the surface of antigen-presenting cells exposes N-glycans that may be targeted by lectins of different structures, specificities, and origins.
29326706	5	11	theme	events	680:685	arg1	sequence					668:675	a same sequence	661:675	a same sequence of events	661:685	In vivo, a same sequence of events follows the administration of an active lectin to mice infected with an intracellular pathogen, conferring resistance to the pathogen.
29326706	10	12	theme	tools	1608:1612	arg1	development					1574:1584	the development	1570:1584	the development of new pharmaceutical tools that promote protective immune responses directed against intracellular pathogens and tumors	1570:1705	This process may lead to the development of new pharmaceutical tools that promote protective immune responses directed against intracellular pathogens and tumors.
29326706	4	13	theme	NF-κB	582:586	arg1	activation					588:597	activation	588:597	activation	588:597	In vitro, these interactions trigger cell signaling that leads to NF-κB activation and production of the Th1 polarizing cytokine IL-12.
29326706	3	14	theme	specificities	488:500	arg1	lectins					455:461	lectins	455:461	lectins of different structures, specificities, and origins	455:513	Expression of the receptors on the surface of antigen-presenting cells exposes N-glycans that may be targeted by lectins of different structures, specificities, and origins.
29326706	3	15	theme	antigen-presenting	388:405	arg1	cells					407:411	antigen-presenting cells	388:411	antigen-presenting cells	388:411	Expression of the receptors on the surface of antigen-presenting cells exposes N-glycans that may be targeted by lectins of different structures, specificities, and origins.
29326706	3	16	from	Expression	342:351	arg1	surface					377:383	the surface	373:383	the surface of antigen-presenting cells	373:411	Expression of the receptors on the surface of antigen-presenting cells exposes N-glycans that may be targeted by lectins of different structures, specificities, and origins.
29326706	5	17	theme	active	720:725	arg1	lectin					727:732	an active lectin	717:732	an active lectin	717:732	In vivo, a same sequence of events follows the administration of an active lectin to mice infected with an intracellular pathogen, conferring resistance to the pathogen.
29326706	9	18	theme	alternative	1410:1420	arg1	way					1422:1424	an alternative way	1407:1424	an alternative way	1407:1424	On the other hand, we showed that TLR-mediated cell activation might be triggered by an alternative way, in which lectins bind to TLRs N-glycans and stimulate cells to increase the expression of pro-inflammatory cytokines.
29326706	8	19	theme	conventional	1243:1254	arg1	patterns					1286:1293	conventional pathogen-associated molecular patterns	1243:1293	conventional pathogen-associated molecular patterns	1243:1293	On the one hand, TLRs act as sensors for the presence of conventional pathogen-associated molecular patterns, such as microbial lipids.
29326706	8	20	theme	microbial	1304:1312	arg1	lipids					1314:1319	microbial lipids	1304:1319	microbial lipids	1304:1319	On the one hand, TLRs act as sensors for the presence of conventional pathogen-associated molecular patterns, such as microbial lipids.
29326706	3	21	theme	cells	407:411	arg1	surface					377:383	the surface	373:383	the surface of antigen-presenting cells	373:411	Expression of the receptors on the surface of antigen-presenting cells exposes N-glycans that may be targeted by lectins of different structures, specificities, and origins.
29326706	2	22	theme	other	279:283	arg1	lectins					285:291	other lectins	279:291	other lectins of microbial origin	279:311	The plant lectin ArtinM, for example, interacts with N-glycans of TLR2, whereas other lectins of microbial origin interact with TLR2 and TLR4.
29326706	4	23	theme	polarizing	625:634	arg1	IL-12					645:649	the Th1 polarizing cytokine IL-12	617:649	the Th1 polarizing cytokine IL-12	617:649	In vitro, these interactions trigger cell signaling that leads to NF-κB activation and production of the Th1 polarizing cytokine IL-12.
29326706	6	24	theme	plant	1064:1068	arg1	ArtinM					1077:1082	the plant lectin ArtinM	1060:1082	the plant lectin ArtinM	1060:1082	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	6	25	dep	gondii	868:873	arg1	TgMIC4					887:892	TgMIC4	887:892	TgMIC4	887:892	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	6	25	dep	gondii	868:873	arg1	TgMIC1					876:881	TgMIC1	876:881	TgMIC1	876:881	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	4	26	theme	Th1	621:623	arg1	IL-12					645:649	the Th1 polarizing cytokine IL-12	617:649	the Th1 polarizing cytokine IL-12	617:649	In vitro, these interactions trigger cell signaling that leads to NF-κB activation and production of the Th1 polarizing cytokine IL-12.
29326706	6	27	dep	in	1014:1015	arg1	vivo					1017:1020	vivo	1017:1020	vivo	1017:1020	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	6	28	theme	TLR4	985:988	arg1	recognition					946:956	recognition	946:956	recognition	946:956	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	6	28	theme	TLR4	985:988	arg1	activation					962:971	activation	962:971	activation	962:971	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	5	29	theme	intracellular	759:771	arg1	pathogen					773:780	an intracellular pathogen	756:780	an intracellular pathogen	756:780	In vivo, a same sequence of events follows the administration of an active lectin to mice infected with an intracellular pathogen, conferring resistance to the pathogen.
29326706	8	30	theme	pathogen-associated	1256:1274	arg1	patterns					1286:1293	conventional pathogen-associated molecular patterns	1243:1293	conventional pathogen-associated molecular patterns	1243:1293	On the one hand, TLRs act as sensors for the presence of conventional pathogen-associated molecular patterns, such as microbial lipids.
29326706	2	31	theme	TLR2	265:268	arg1	N-glycans					252:260	N-glycans	252:260	N-glycans of TLR2	252:268	The plant lectin ArtinM, for example, interacts with N-glycans of TLR2, whereas other lectins of microbial origin interact with TLR2 and TLR4.
29326706	1	32	theme	immunomodulatory	170:185	arg1	properties					187:196	immunomodulatory properties	170:196	immunomodulatory properties	170:196	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	1	32	theme	immunomodulatory	170:185	arg1	lectins					107:113	some lectins	102:113	some lectins	102:113	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	1	32	theme	immunomodulatory	170:185	arg1	agonists					122:129	agonists	122:129	agonists of toll-like receptors (TLRs)	122:159	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	0	33	theme	Receptors	39:47	arg1	Recognition					14:24	Recognition	14:24	Recognition	14:24	Targeting and Recognition of Toll-Like Receptors by Plant and Pathogen Lectins.
29326706	0	33	theme	Receptors	39:47	arg1	Targeting					0:8	Targeting	0:8	Targeting	0:8	Targeting and Recognition of Toll-Like Receptors by Plant and Pathogen Lectins.
29326706	9	34	theme	TLRs	1452:1455	arg1	N-glycans					1457:1465	TLRs N-glycans	1452:1465	TLRs N-glycans	1452:1465	On the other hand, we showed that TLR-mediated cell activation might be triggered by an alternative way, in which lectins bind to TLRs N-glycans and stimulate cells to increase the expression of pro-inflammatory cytokines.
29326706	7	35	theme	antigen-presenting	1160:1177	arg1	cells					1179:1183	antigen-presenting cells	1160:1183	antigen-presenting cells	1160:1183	In this article, we highlight these two distinct mechanisms for activating antigen-presenting cells.
29326706	0	36	dep	Plant	52:56	arg1	Lectins					71:77	Lectins	71:77	Lectins	71:77	Targeting and Recognition of Toll-Like Receptors by Plant and Pathogen Lectins.
29326706	6	37	theme	TLR2	976:979	arg1	recognition					946:956	recognition	946:956	recognition	946:956	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	6	37	theme	TLR2	976:979	arg1	activation					962:971	activation	962:971	activation	962:971	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	0	38	theme	Toll-Like	29:37	arg1	Receptors					39:47	Toll-Like Receptors	29:47	Toll-Like Receptors	29:47	Targeting and Recognition of Toll-Like Receptors by Plant and Pathogen Lectins.
29326706	5	39	theme	same	663:666	arg1	sequence					668:675	a same sequence	661:675	a same sequence of events	661:685	In vivo, a same sequence of events follows the administration of an active lectin to mice infected with an intracellular pathogen, conferring resistance to the pathogen.
29326706	5	40	theme	lectin	727:732	arg1	administration					699:712	the administration	695:712	the administration of an active lectin to mice infected with an intracellular pathogen	695:780	In vivo, a same sequence of events follows the administration of an active lectin to mice infected with an intracellular pathogen, conferring resistance to the pathogen.
29326706	2	41	theme	lectin	209:214	arg1	ArtinM					216:221	The plant lectin ArtinM	199:221	The plant lectin ArtinM	199:221	The plant lectin ArtinM, for example, interacts with N-glycans of TLR2, whereas other lectins of microbial origin interact with TLR2 and TLR4.
29326706	10	42	theme	protective	1627:1636	arg1	responses					1645:1653	protective immune responses	1627:1653	protective immune responses directed against intracellular pathogens and tumors	1627:1705	This process may lead to the development of new pharmaceutical tools that promote protective immune responses directed against intracellular pathogens and tumors.
29326706	8	43	theme	patterns	1286:1293	arg1	presence					1231:1238	the presence	1227:1238	the presence of conventional pathogen-associated molecular patterns	1227:1293	On the one hand, TLRs act as sensors for the presence of conventional pathogen-associated molecular patterns, such as microbial lipids.
29326706	2	44	theme	plant	203:207	arg1	ArtinM					216:221	The plant lectin ArtinM	199:221	The plant lectin ArtinM	199:221	The plant lectin ArtinM, for example, interacts with N-glycans of TLR2, whereas other lectins of microbial origin interact with TLR2 and TLR4.
29326706	8	45	theme	molecular	1276:1284	arg1	patterns					1286:1293	conventional pathogen-associated molecular patterns	1243:1293	conventional pathogen-associated molecular patterns	1243:1293	On the one hand, TLRs act as sensors for the presence of conventional pathogen-associated molecular patterns, such as microbial lipids.
29326706	2	46	theme	origin	306:311	arg1	lectins					285:291	other lectins	279:291	other lectins of microbial origin	279:311	The plant lectin ArtinM, for example, interacts with N-glycans of TLR2, whereas other lectins of microbial origin interact with TLR2 and TLR4.
29326706	3	47	theme	receptors	360:368	arg1	Expression					342:351	Expression	342:351	Expression of the receptors on the surface of antigen-presenting cells	342:411	Expression of the receptors on the surface of antigen-presenting cells exposes N-glycans that may be targeted by lectins of different structures, specificities, and origins.
29326706	10	48	theme	pharmaceutical	1593:1606	arg1	tools					1608:1612	new pharmaceutical tools	1589:1612	new pharmaceutical tools that promote protective immune responses directed against intracellular pathogens and tumors	1589:1705	This process may lead to the development of new pharmaceutical tools that promote protective immune responses directed against intracellular pathogens and tumors.
29326706	9	49	theme	cell	1369:1372	arg1	activation					1374:1383	TLR-mediated cell activation	1356:1383	TLR-mediated cell activation	1356:1383	On the other hand, we showed that TLR-mediated cell activation might be triggered by an alternative way, in which lectins bind to TLRs N-glycans and stimulate cells to increase the expression of pro-inflammatory cytokines.
29326706	2	50	theme	microbial	296:304	arg1	origin					306:311	microbial origin	296:311	microbial origin	296:311	The plant lectin ArtinM, for example, interacts with N-glycans of TLR2, whereas other lectins of microbial origin interact with TLR2 and TLR4.
29326706	10	51	theme	new	1589:1591	arg1	tools					1608:1612	new pharmaceutical tools	1589:1612	new pharmaceutical tools that promote protective immune responses directed against intracellular pathogens and tumors	1589:1705	This process may lead to the development of new pharmaceutical tools that promote protective immune responses directed against intracellular pathogens and tumors.
29326706	10	52	theme	immune	1638:1643	arg1	responses					1645:1653	protective immune responses	1627:1653	protective immune responses directed against intracellular pathogens and tumors	1627:1705	This process may lead to the development of new pharmaceutical tools that promote protective immune responses directed against intracellular pathogens and tumors.
29326706	6	53	dep	lectins	826:832	arg1	gondii					868:873	Toxoplasma gondii	857:873	Toxoplasma gondii (TgMIC1 and TgMIC4)	857:893	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	6	53	dep	lectins	826:832	arg1	Paracoccin					930:939	Paracoccin	930:939	Paracoccin	930:939	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	6	53	dep	lectins	826:832	arg1	brasiliensis					916:927	Paracoccidioides brasiliensis	899:927	Paracoccidioides brasiliensis (Paracoccin)	899:940	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	6	54	theme	comparable	1030:1039	arg1	events					1003:1008	cell events	998:1008	cell events	998:1008	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	7	55	theme	distinct	1125:1132	arg1	mechanisms					1134:1143	these two distinct mechanisms	1115:1143	these two distinct mechanisms	1115:1143	In this article, we highlight these two distinct mechanisms for activating antigen-presenting cells.
29326706	1	56	contain	have	165:168	arg2	properties					187:196	immunomodulatory properties	170:196	immunomodulatory properties	170:196	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	1	56	contain	have	165:168	arg1	agonists					122:129	agonists	122:129	agonists of toll-like receptors (TLRs)	122:159	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	1	56	contain	have	165:168	arg2	lectins					107:113	some lectins	102:113	some lectins	102:113	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	1	56	contain	have	165:168	arg1	properties					187:196	immunomodulatory properties	170:196	immunomodulatory properties	170:196	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	1	56	contain	have	165:168	arg1	lectins					107:113	some lectins	102:113	some lectins	102:113	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	1	56	contain	have	165:168	arg2	agonists					122:129	agonists	122:129	agonists of toll-like receptors (TLRs)	122:159	We have reported that some lectins act as agonists of toll-like receptors (TLRs) and have immunomodulatory properties.
29326706	9	57	theme	pro-inflammatory	1517:1532	arg1	cytokines					1534:1542	pro-inflammatory cytokines	1517:1542	pro-inflammatory cytokines	1517:1542	On the other hand, we showed that TLR-mediated cell activation might be triggered by an alternative way, in which lectins bind to TLRs N-glycans and stimulate cells to increase the expression of pro-inflammatory cytokines.
29326706	9	58	theme	TLR-mediated	1356:1367	arg1	activation					1374:1383	TLR-mediated cell activation	1356:1383	TLR-mediated cell activation	1356:1383	On the other hand, we showed that TLR-mediated cell activation might be triggered by an alternative way, in which lectins bind to TLRs N-glycans and stimulate cells to increase the expression of pro-inflammatory cytokines.
29326706	3	59	theme	structures	476:485	arg1	lectins					455:461	lectins	455:461	lectins of different structures, specificities, and origins	455:513	Expression of the receptors on the surface of antigen-presenting cells exposes N-glycans that may be targeted by lectins of different structures, specificities, and origins.
29326706	6	60	theme	cell	998:1001	arg1	events					1003:1008	cell events	998:1008	cell events	998:1008	The lectins of the human pathogens Toxoplasma gondii (TgMIC1 and TgMIC4) and Paracoccidioides brasiliensis (Paracoccin), by recognition and activation of TLR2 and TLR4, induce cell events and in vivo effects comparable to the promoted by the plant lectin ArtinM.
29326706	4	61	theme	cell	553:556	arg1	signaling					558:566	cell signaling	553:566	cell signaling that leads to NF-κB activation and production of the Th1 polarizing cytokine IL-12	553:649	In vitro, these interactions trigger cell signaling that leads to NF-κB activation and production of the Th1 polarizing cytokine IL-12.
29326706	9	62	theme	cytokines	1534:1542	arg1	expression					1503:1512	the expression	1499:1512	the expression of pro-inflammatory cytokines	1499:1542	On the other hand, we showed that TLR-mediated cell activation might be triggered by an alternative way, in which lectins bind to TLRs N-glycans and stimulate cells to increase the expression of pro-inflammatory cytokines.
29326706	4	63	theme	cytokine	636:643	arg1	IL-12					645:649	the Th1 polarizing cytokine IL-12	617:649	the Th1 polarizing cytokine IL-12	617:649	In vitro, these interactions trigger cell signaling that leads to NF-κB activation and production of the Th1 polarizing cytokine IL-12.
29138307	6	0	theme	grassland	1331:1339	arg1	litter					1346:1351	grassland leaf litter	1331:1351	grassland leaf litter	1331:1351	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	2	1	theme	relevant	372:379	arg1	traits					381:386	ecologically relevant traits	359:386	ecologically relevant traits	359:386	However, it is unclear how variation within this microdiversity influences ecologically relevant traits.
29138307	1	2	theme	gene	268:271	arg1	sequences					273:281	16S rRNA gene sequences	259:281	16S rRNA gene sequences	259:281	Much genetic diversity within a bacterial community is likely obscured by microdiversity within operational taxonomic units (OTUs) defined by 16S rRNA gene sequences.
29138307	14	3	theme	preference	2660:2669	arg1	degree					2615:2620	the degree	2611:2620	the degree of carbohydrate usage and temperature preference	2611:2669	We find that the degree of carbohydrate usage and temperature preference vary within the OTU, whereas its responses to changes in precipitation are relatively uniform.
29138307	3	4	theme	community	556:564	arg1	%					537:537	7.8%	534:537	7.8% of the bacterial community at a grassland site undergoing global change manipulations	534:623	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	3	4	theme	community	556:564	arg1	community					556:564	the bacterial community	542:564	the bacterial community at a grassland site undergoing global change manipulations	542:623	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	5	5	theme	global	901:906	arg1	change					908:913	the global change	897:913	the global change	897:913	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	10	6	theme	genetic	2084:2090	arg1	level					2092:2096	a broad genetic level	2076:2096	a broad genetic level	2076:2096	Indeed, most studies, particularly studies of soil, assess community composition at a broad genetic level by classifying diversity into taxa (OTUs) defined by 16S rRNA sequence similarity.
29138307	3	7	theme	global	597:602	arg1	manipulations					611:623	global change manipulations	597:623	global change manipulations	597:623	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	1	8	theme	operational	213:223	arg1	units					235:239	operational taxonomic units	213:239	operational taxonomic units (OTUs) defined by 16S rRNA gene sequences	213:281	Much genetic diversity within a bacterial community is likely obscured by microdiversity within operational taxonomic units (OTUs) defined by 16S rRNA gene sequences.
29138307	1	8	theme	operational	213:223	arg1	OTUs					242:245	OTUs	242:245	OTUs	242:245	Much genetic diversity within a bacterial community is likely obscured by microdiversity within operational taxonomic units (OTUs) defined by 16S rRNA gene sequences.
29138307	5	9	theme	water	993:997	arg1	availability					999:1010	decreased water availability	983:1010	decreased water availability	983:1010	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	5	10	theme	seasonal	872:879	arg1	variability					881:891	seasonal variability	872:891	seasonal variability	872:891	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	11	11	theme	fine-scale	2267:2276	arg1	differentiation					2289:2303	fine-scale ecological differentiation	2267:2303	fine-scale ecological differentiation among closely related strains	2267:2333	However, these classifications potentially obscure variation in traits that result in fine-scale ecological differentiation among closely related strains.
29138307	0	12	from	Variation	74:82	arg1	Traits					109:114	Ecologically Relevant Traits	87:114	Ecologically Relevant Traits	87:114	Microdiversity of an Abundant Terrestrial Bacterium Encompasses Extensive Variation in Ecologically Relevant Traits.
29138307	4	13	theme	lab-based	738:746	arg1	assays					762:767	lab-based physiological assays	738:767	metagenomic data, collected in situ over 2 years, together with lab-based physiological assays	674:767	We use cultured bacterial isolates to interpret metagenomic data, collected in situ over 2 years, together with lab-based physiological assays to determine the extent of trait variation within this abundant OTU.
29138307	11	14	from	variation	2232:2240	arg1	traits					2245:2250	traits	2245:2250	traits that result in fine-scale ecological differentiation among closely related strains	2245:2333	However, these classifications potentially obscure variation in traits that result in fine-scale ecological differentiation among closely related strains.
29138307	5	15	theme	relative	958:965	arg1	abundance					967:975	relative abundance	958:975	relative abundance under decreased water availability	958:1010	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	9	16	theme	plummeting	1823:1832	arg1	costs					1834:1838	the plummeting costs	1819:1838	the plummeting costs of sequencing	1819:1852	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	7	17	theme	temperature	1406:1416	arg1	preference					1418:1427	temperature preference	1406:1427	temperature preference	1406:1427	However, the degree of carbohydrate utilization and temperature preference for this degradation varied greatly among clades.
29138307	6	18	from	analyses	1124:1131	arg1	lab					1140:1142	the lab	1136:1142	the lab	1136:1142	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	3	19	from	site	581:584	arg1	%					537:537	7.8%	534:537	7.8% of the bacterial community at a grassland site undergoing global change manipulations	534:623	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	3	19	from	site	581:584	arg1	community					556:564	the bacterial community	542:564	the bacterial community at a grassland site undergoing global change manipulations	542:623	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	15	20	theme	soil	2840:2843	arg1	diversity					2855:2863	soil bacterial diversity	2840:2863	soil bacterial diversity	2840:2863	These results suggest that microdiversity may be key to understanding how soil bacterial diversity is linked to ecosystem functioning.
29138307	1	21	theme	bacterial	149:157	arg1	community					159:167	a bacterial community	147:167	a bacterial community	147:167	Much genetic diversity within a bacterial community is likely obscured by microdiversity within operational taxonomic units (OTUs) defined by 16S rRNA gene sequences.
29138307	7	22	theme	utilization	1390:1400	arg1	degree					1367:1372	the degree	1363:1372	the degree of carbohydrate utilization and temperature preference for this degradation	1363:1448	However, the degree of carbohydrate utilization and temperature preference for this degradation varied greatly among clades.
29138307	12	23	theme	Californian	2459:2469	arg1	grassland					2471:2479	a southern Californian grassland	2448:2479	a southern Californian grassland	2448:2479	Here, we investigated "microdiversity" in a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland).
29138307	11	24	theme	ecological	2278:2287	arg1	differentiation					2289:2303	fine-scale ecological differentiation	2267:2303	fine-scale ecological differentiation among closely related strains	2267:2333	However, these classifications potentially obscure variation in traits that result in fine-scale ecological differentiation among closely related strains.
29138307	8	25	theme	phylogenetic	1557:1568	arg1	depths					1570:1575	different phylogenetic depths	1547:1575	different phylogenetic depths	1547:1575	Overall, we find that traits within Curtobacterium are conserved at different phylogenetic depths.
29138307	10	26	theme	16S	2151:2153	arg1	similarity					2169:2178	16S rRNA sequence similarity	2151:2178	16S rRNA sequence similarity	2151:2178	Indeed, most studies, particularly studies of soil, assess community composition at a broad genetic level by classifying diversity into taxa (OTUs) defined by 16S rRNA sequence similarity.
29138307	9	27	from	bacteria	1607:1614	arg1	systems					1626:1632	marine systems	1619:1632	marine systems	1619:1632	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	12	28	theme	soil	2420:2423	arg1	system					2425:2430	a highly diverse and poorly characterized soil system	2378:2430	a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland)	2378:2480	Here, we investigated "microdiversity" in a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland).
29138307	4	29	theme	trait	796:800	arg1	variation					802:810	trait variation	796:810	trait variation within this abundant OTU	796:835	We use cultured bacterial isolates to interpret metagenomic data, collected in situ over 2 years, together with lab-based physiological assays to determine the extent of trait variation within this abundant OTU.
29138307	14	30	theme	temperature	2648:2658	arg1	preference					2660:2669	temperature preference	2648:2669	temperature preference	2648:2669	We find that the degree of carbohydrate usage and temperature preference vary within the OTU, whereas its responses to changes in precipitation are relatively uniform.
29138307	9	31	theme	genetic	1885:1891	arg1	diversity					1893:1901	the fine-scale genetic diversity	1870:1901	the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge	1870:1989	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	7	32	theme	carbohydrate	1377:1388	arg1	utilization					1390:1400	carbohydrate utilization	1377:1400	carbohydrate utilization	1377:1400	However, the degree of carbohydrate utilization and temperature preference for this degradation varied greatly among clades.
29138307	12	33	theme	diverse	2387:2393	arg1	system					2425:2430	a highly diverse and poorly characterized soil system	2378:2430	a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland)	2378:2480	Here, we investigated "microdiversity" in a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland).
29138307	9	34	theme	diverse	1635:1641	arg1	microbes					1643:1650	diverse microbes	1635:1650	diverse microbes within this taxon	1635:1668	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	9	35	theme	microbial	1908:1916	arg1	community-and					1918:1930	a microbial community-and	1906:1930	a microbial community-and interpreting its functional importance-remains a challenge	1906:1989	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	5	36	theme	Curtobacterium	854:867	arg1	response					842:849	The response	838:849	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability,	838:1011	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	1	37	theme	genetic	122:128	arg1	diversity					130:138	Much genetic diversity	117:138	Much genetic diversity within a bacterial community	117:167	Much genetic diversity within a bacterial community is likely obscured by microdiversity within operational taxonomic units (OTUs) defined by 16S rRNA gene sequences.
29138307	3	38	theme	leaf	479:482	arg1	bacterium					491:499	the dominant leaf litter bacterium	466:499	the dominant leaf litter bacterium	466:499	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	0	39	theme	Terrestrial	30:40	arg1	Bacterium					42:50	an Abundant Terrestrial Bacterium	18:50	an Abundant Terrestrial Bacterium	18:50	Microdiversity of an Abundant Terrestrial Bacterium Encompasses Extensive Variation in Ecologically Relevant Traits.
29138307	12	40	dep	system	2425:2430	arg1	litter					2438:2443	leaf litter	2433:2443	leaf litter in a southern Californian grassland	2433:2479	Here, we investigated "microdiversity" in a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland).
29138307	12	41	from	litter	2438:2443	arg1	grassland					2471:2479	a southern Californian grassland	2448:2479	a southern Californian grassland	2448:2479	Here, we investigated "microdiversity" in a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland).
29138307	14	42	theme	carbohydrate	2625:2636	arg1	usage					2638:2642	carbohydrate usage	2625:2642	carbohydrate usage	2625:2642	We find that the degree of carbohydrate usage and temperature preference vary within the OTU, whereas its responses to changes in precipitation are relatively uniform.
29138307	9	43	theme	functional	1949:1958	arg1	challenge					1981:1989	its functional importance-remains a challenge	1945:1989	its functional importance-remains a challenge	1945:1989	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	13	44	theme	abundant	2506:2513	arg1	bacterium					2515:2523	the most abundant bacterium	2497:2523	the most abundant bacterium	2497:2523	We focused on the most abundant bacterium, Curtobacterium, which by standard methods is grouped into only one OTU.
29138307	6	45	theme	carbohydrates	1192:1204	arg1	degradation					1158:1168	degradation	1158:1168	degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan,	1158:1245	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	9	46	theme	a	1979:1979	arg1	challenge					1981:1989	its functional importance-remains a challenge	1945:1989	its functional importance-remains a challenge	1945:1989	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	4	47	theme	abundant	824:831	arg1	OTU					833:835	this abundant OTU	819:835	this abundant OTU	819:835	We use cultured bacterial isolates to interpret metagenomic data, collected in situ over 2 years, together with lab-based physiological assays to determine the extent of trait variation within this abundant OTU.
29138307	6	48	theme	abundant	1173:1180	arg1	xylan					1240:1244	xylan	1240:1244	xylan	1240:1244	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	6	48	theme	abundant	1173:1180	arg1	cellulose					1226:1234	cellulose	1226:1234	cellulose	1226:1234	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	6	48	theme	abundant	1173:1180	arg1	carbohydrates					1192:1204	abundant polymeric carbohydrates	1173:1204	abundant polymeric carbohydrates within leaf litter	1173:1223	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	10	49	theme	sequence	2160:2167	arg1	similarity					2169:2178	16S rRNA sequence similarity	2151:2178	16S rRNA sequence similarity	2151:2178	Indeed, most studies, particularly studies of soil, assess community composition at a broad genetic level by classifying diversity into taxa (OTUs) defined by 16S rRNA sequence similarity.
29138307	3	50	theme	multifaceted	407:418	arg1	approach					420:427	a multifaceted approach	405:427	a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations	405:623	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	4	51	theme	bacterial	642:650	arg1	isolates					652:659	cultured bacterial isolates	633:659	cultured bacterial isolates	633:659	We use cultured bacterial isolates to interpret metagenomic data, collected in situ over 2 years, together with lab-based physiological assays to determine the extent of trait variation within this abundant OTU.
29138307	6	52	theme	leaf	1341:1344	arg1	litter					1346:1351	grassland leaf litter	1331:1351	grassland leaf litter	1331:1351	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	1	53	theme	rRNA	263:266	arg1	sequences					273:281	16S rRNA gene sequences	259:281	16S rRNA gene sequences	259:281	Much genetic diversity within a bacterial community is likely obscured by microdiversity within operational taxonomic units (OTUs) defined by 16S rRNA gene sequences.
29138307	3	54	from	%	537:537	arg1	site					581:584	a grassland site	569:584	a grassland site undergoing global change manipulations	569:623	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	11	55	theme	obscure	2224:2230	arg1	variation					2232:2240	obscure variation	2224:2240	obscure variation in traits that result in fine-scale ecological differentiation among closely related strains	2224:2333	However, these classifications potentially obscure variation in traits that result in fine-scale ecological differentiation among closely related strains.
29138307	10	56	theme	community	2051:2059	arg1	composition					2061:2071	community composition	2051:2071	community composition	2051:2071	Indeed, most studies, particularly studies of soil, assess community composition at a broad genetic level by classifying diversity into taxa (OTUs) defined by 16S rRNA sequence similarity.
29138307	6	57	theme	high	1313:1316	arg1	abundance					1318:1326	its high abundance	1309:1326	its high abundance in grassland leaf litter	1309:1351	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	3	58	theme	bacterial	546:554	arg1	community					556:564	the bacterial community	542:564	the bacterial community at a grassland site undergoing global change manipulations	542:623	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	12	59	from	"	2373:2373	arg1	system					2425:2430	a highly diverse and poorly characterized soil system	2378:2430	a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland)	2378:2480	Here, we investigated "microdiversity" in a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland).
29138307	11	60	theme	related	2319:2325	arg1	strains					2327:2333	closely related strains	2311:2333	closely related strains	2311:2333	However, these classifications potentially obscure variation in traits that result in fine-scale ecological differentiation among closely related strains.
29138307	9	61	theme	Curtobacterium	1739:1752	arg1	abundance					1754:1762	Curtobacterium abundance	1739:1762	Curtobacterium abundance	1739:1762	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	5	62	theme	change	908:913	arg1	increase					946:953	specifically an increase	930:953	specifically an increase in relative abundance under decreased water availability	930:1010	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	5	62	theme	change	908:913	arg1	manipulations					915:927	seasonal variability and the global change manipulations	872:927	seasonal variability and the global change manipulations	872:927	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	9	63	from	abundance	1754:1762	arg1	environment.IMPORTANCE					1788:1809	the environment.IMPORTANCE	1784:1809	the environment.IMPORTANCE	1784:1809	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	10	64	theme	broad	2078:2082	arg1	level					2092:2096	a broad genetic level	2076:2096	a broad genetic level	2076:2096	Indeed, most studies, particularly studies of soil, assess community composition at a broad genetic level by classifying diversity into taxa (OTUs) defined by 16S rRNA sequence similarity.
29138307	3	65	theme	grassland	571:579	arg1	site					581:584	a grassland site	569:584	a grassland site undergoing global change manipulations	569:623	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	1	66	theme	taxonomic	225:233	arg1	units					235:239	operational taxonomic units	213:239	operational taxonomic units (OTUs) defined by 16S rRNA gene sequences	213:281	Much genetic diversity within a bacterial community is likely obscured by microdiversity within operational taxonomic units (OTUs) defined by 16S rRNA gene sequences.
29138307	1	66	theme	taxonomic	225:233	arg1	OTUs					242:245	OTUs	242:245	OTUs	242:245	Much genetic diversity within a bacterial community is likely obscured by microdiversity within operational taxonomic units (OTUs) defined by 16S rRNA gene sequences.
29138307	12	67	theme	southern	2450:2457	arg1	grassland					2471:2479	a southern Californian grassland	2448:2479	a southern Californian grassland	2448:2479	Here, we investigated "microdiversity" in a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland).
29138307	6	68	theme	leaf	1213:1216	arg1	litter					1218:1223	leaf litter	1213:1223	leaf litter	1213:1223	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	5	69	theme	variability	881:891	arg1	increase					946:953	specifically an increase	930:953	specifically an increase in relative abundance under decreased water availability	930:1010	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	5	69	theme	variability	881:891	arg1	manipulations					915:927	seasonal variability and the global change manipulations	872:927	seasonal variability and the global change manipulations	872:927	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	4	70	theme	metagenomic	674:684	arg1	data					686:689	metagenomic data	674:689	metagenomic data	674:689	We use cultured bacterial isolates to interpret metagenomic data, collected in situ over 2 years, together with lab-based physiological assays to determine the extent of trait variation within this abundant OTU.
29138307	14	71	from	changes	2717:2723	arg1	precipitation					2728:2740	precipitation	2728:2740	precipitation	2728:2740	We find that the degree of carbohydrate usage and temperature preference vary within the OTU, whereas its responses to changes in precipitation are relatively uniform.
29138307	3	72	theme	change	604:609	arg1	manipulations					611:623	global change manipulations	597:623	global change manipulations	597:623	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	12	73	theme	leaf	2433:2436	arg1	litter					2438:2443	leaf litter	2433:2443	leaf litter in a southern Californian grassland	2433:2479	Here, we investigated "microdiversity" in a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland).
29138307	8	74	theme	different	1547:1555	arg1	depths					1570:1575	different phylogenetic depths	1547:1575	different phylogenetic depths	1547:1575	Overall, we find that traits within Curtobacterium are conserved at different phylogenetic depths.
29138307	7	75	theme	preference	1418:1427	arg1	degree					1367:1372	the degree	1363:1372	the degree of carbohydrate utilization and temperature preference for this degradation	1363:1448	However, the degree of carbohydrate utilization and temperature preference for this degradation varied greatly among clades.
29138307	4	76	theme	physiological	748:760	arg1	assays					762:767	lab-based physiological assays	738:767	metagenomic data, collected in situ over 2 years, together with lab-based physiological assays	674:767	We use cultured bacterial isolates to interpret metagenomic data, collected in situ over 2 years, together with lab-based physiological assays to determine the extent of trait variation within this abundant OTU.
29138307	9	77	theme	sequencing	1843:1852	arg1	costs					1834:1838	the plummeting costs	1819:1838	the plummeting costs of sequencing	1819:1852	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	12	78	theme	characterized	2406:2418	arg1	system					2425:2430	a highly diverse and poorly characterized soil system	2378:2430	a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland)	2378:2480	Here, we investigated "microdiversity" in a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland).
29138307	0	79	theme	Relevant	100:107	arg1	Traits					109:114	Ecologically Relevant Traits	87:114	Ecologically Relevant Traits	87:114	Microdiversity of an Abundant Terrestrial Bacterium Encompasses Extensive Variation in Ecologically Relevant Traits.
29138307	5	80	from	increase	946:953	arg1	abundance					967:975	relative abundance	958:975	relative abundance under decreased water availability	958:1010	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	4	81	dep	together	724:731	arg1	with					733:736	with	733:736	with	733:736	We use cultured bacterial isolates to interpret metagenomic data, collected in situ over 2 years, together with lab-based physiological assays to determine the extent of trait variation within this abundant OTU.
29138307	9	82	from	distribution	1768:1779	arg1	environment.IMPORTANCE					1788:1809	the environment.IMPORTANCE	1784:1809	the environment.IMPORTANCE	1784:1809	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	9	83	theme	fine-scale	1874:1883	arg1	diversity					1893:1901	the fine-scale genetic diversity	1870:1901	the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge	1870:1989	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	12	84	theme	microdiversity	2359:2372	arg1	"					2373:2373	"microdiversity"	2358:2373	"microdiversity" in a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland)	2358:2480	Here, we investigated "microdiversity" in a highly diverse and poorly characterized soil system (leaf litter in a southern Californian grassland).
29138307	9	85	theme	marine	1619:1624	arg1	systems					1626:1632	marine systems	1619:1632	marine systems	1619:1632	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	15	86	attach	linked	2868:2873	arg2	diversity					2855:2863	soil bacterial diversity	2840:2863	soil bacterial diversity	2840:2863	These results suggest that microdiversity may be key to understanding how soil bacterial diversity is linked to ecosystem functioning.
29138307	15	86	attach	linked	2868:2873	arg1	ecosystem					2878:2886	ecosystem	2878:2886	ecosystem functioning	2878:2898	These results suggest that microdiversity may be key to understanding how soil bacterial diversity is linked to ecosystem functioning.
29138307	4	87	theme	variation	802:810	arg1	extent					786:791	the extent	782:791	the extent of trait variation within this abundant OTU	782:835	We use cultured bacterial isolates to interpret metagenomic data, collected in situ over 2 years, together with lab-based physiological assays to determine the extent of trait variation within this abundant OTU.
29138307	3	88	theme	dominant	470:477	arg1	bacterium					491:499	the dominant leaf litter bacterium	466:499	the dominant leaf litter bacterium	466:499	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	15	89	theme	bacterial	2845:2853	arg1	diversity					2855:2863	soil bacterial diversity	2840:2863	soil bacterial diversity	2840:2863	These results suggest that microdiversity may be key to understanding how soil bacterial diversity is linked to ecosystem functioning.
29138307	5	90	theme	decreased	983:991	arg1	availability					999:1010	decreased water availability	983:1010	decreased water availability	983:1010	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	0	91	theme	Abundant	21:28	arg1	Bacterium					42:50	an Abundant Terrestrial Bacterium	18:50	an Abundant Terrestrial Bacterium	18:50	Microdiversity of an Abundant Terrestrial Bacterium Encompasses Extensive Variation in Ecologically Relevant Traits.
29138307	5	92	theme	Curtobacterium	1049:1062	arg1	lineages					1064:1071	six Curtobacterium lineages	1045:1071	six Curtobacterium lineages identified at this site	1045:1095	The response of Curtobacterium to seasonal variability and the global change manipulations, specifically an increase in relative abundance under decreased water availability, appeared to be conserved across six Curtobacterium lineages identified at this site.
29138307	1	93	theme	Much	117:120	arg1	diversity					130:138	Much genetic diversity	117:138	Much genetic diversity within a bacterial community	117:167	Much genetic diversity within a bacterial community is likely obscured by microdiversity within operational taxonomic units (OTUs) defined by 16S rRNA gene sequences.
29138307	6	94	theme	physiological	1110:1122	arg1	analyses					1124:1131	Genomic and physiological analyses	1098:1131	Genomic and physiological analyses in the lab	1098:1142	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	9	95	theme	community-and	1918:1930	arg1	diversity					1893:1901	the fine-scale genetic diversity	1870:1901	the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge	1870:1989	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	13	96	theme	standard	2551:2558	arg1	methods					2560:2566	standard methods	2551:2566	standard methods	2551:2566	We focused on the most abundant bacterium, Curtobacterium, which by standard methods is grouped into only one OTU.
29138307	0	97	theme	Bacterium	42:50	arg1	Microdiversity					0:13	Microdiversity	0:13	Microdiversity of an Abundant Terrestrial Bacterium	0:50	Microdiversity of an Abundant Terrestrial Bacterium Encompasses Extensive Variation in Ecologically Relevant Traits.
29138307	14	98	theme	usage	2638:2642	arg1	degree					2615:2620	the degree	2611:2620	the degree of carbohydrate usage and temperature preference	2611:2669	We find that the degree of carbohydrate usage and temperature preference vary within the OTU, whereas its responses to changes in precipitation are relatively uniform.
29138307	6	99	theme	Genomic	1098:1104	arg1	analyses					1124:1131	Genomic and physiological analyses	1098:1131	Genomic and physiological analyses in the lab	1098:1142	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	3	100	theme	litter	484:489	arg1	bacterium					491:499	the dominant leaf litter bacterium	466:499	the dominant leaf litter bacterium	466:499	Here, we employ a multifaceted approach to investigate microdiversity within the dominant leaf litter bacterium, Curtobacterium, which comprises 7.8% of the bacterial community at a grassland site undergoing global change manipulations.
29138307	0	101	theme	Extensive	64:72	arg1	Variation					74:82	Extensive Variation	64:82	Extensive Variation in Ecologically Relevant Traits	64:114	Microdiversity of an Abundant Terrestrial Bacterium Encompasses Extensive Variation in Ecologically Relevant Traits.
29138307	9	102	theme	importance-remains	1960:1977	arg1	challenge					1981:1989	its functional importance-remains a challenge	1945:1989	its functional importance-remains a challenge	1945:1989	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	6	103	theme	polymeric	1182:1190	arg1	xylan					1240:1244	xylan	1240:1244	xylan	1240:1244	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	6	103	theme	polymeric	1182:1190	arg1	cellulose					1226:1234	cellulose	1226:1234	cellulose	1226:1234	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	6	103	theme	polymeric	1182:1190	arg1	carbohydrates					1192:1204	abundant polymeric carbohydrates	1173:1204	abundant polymeric carbohydrates within leaf litter	1173:1223	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	4	104	theme	cultured	633:640	arg1	isolates					652:659	cultured bacterial isolates	633:659	cultured bacterial isolates	633:659	We use cultured bacterial isolates to interpret metagenomic data, collected in situ over 2 years, together with lab-based physiological assays to determine the extent of trait variation within this abundant OTU.
29138307	10	105	theme	most	2000:2003	arg1	studies					2005:2011	most studies	2000:2011	most studies	2000:2011	Indeed, most studies, particularly studies of soil, assess community composition at a broad genetic level by classifying diversity into taxa (OTUs) defined by 16S rRNA sequence similarity.
29138307	10	105	theme	most	2000:2003	arg1	studies					2027:2033	studies	2027:2033	particularly studies of soil	2014:2041	Indeed, most studies, particularly studies of soil, assess community composition at a broad genetic level by classifying diversity into taxa (OTUs) defined by 16S rRNA sequence similarity.
29138307	6	106	from	abundance	1318:1326	arg1	litter					1346:1351	grassland leaf litter	1331:1351	grassland leaf litter	1331:1351	Genomic and physiological analyses in the lab revealed that degradation of abundant polymeric carbohydrates within leaf litter, cellulose and xylan, is nearly universal across the genus, which may contribute to its high abundance in grassland leaf litter.
29138307	9	107	theme	distinct	1691:1698	arg1	ecotypes					1700:1707	distinct ecotypes	1691:1707	distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge	1691:1989	We speculate that similar to bacteria in marine systems, diverse microbes within this taxon may be structured in distinct ecotypes that are key to understanding Curtobacterium abundance and distribution in the environment.IMPORTANCE Despite the plummeting costs of sequencing, characterizing the fine-scale genetic diversity of a microbial community-and interpreting its functional importance-remains a challenge.
29138307	10	108	theme	rRNA	2155:2158	arg1	similarity					2169:2178	16S rRNA sequence similarity	2151:2178	16S rRNA sequence similarity	2151:2178	Indeed, most studies, particularly studies of soil, assess community composition at a broad genetic level by classifying diversity into taxa (OTUs) defined by 16S rRNA sequence similarity.
29138307	10	109	theme	soil	2038:2041	arg1	studies					2005:2011	most studies	2000:2011	most studies	2000:2011	Indeed, most studies, particularly studies of soil, assess community composition at a broad genetic level by classifying diversity into taxa (OTUs) defined by 16S rRNA sequence similarity.
29138307	10	109	theme	soil	2038:2041	arg1	studies					2027:2033	studies	2027:2033	particularly studies of soil	2014:2041	Indeed, most studies, particularly studies of soil, assess community composition at a broad genetic level by classifying diversity into taxa (OTUs) defined by 16S rRNA sequence similarity.
29138307	1	110	theme	16S	259:261	arg1	sequences					273:281	16S rRNA gene sequences	259:281	16S rRNA gene sequences	259:281	Much genetic diversity within a bacterial community is likely obscured by microdiversity within operational taxonomic units (OTUs) defined by 16S rRNA gene sequences.
29103485	2	0	theme	cellulose/hydroxyapatite	488:511	arg1	scaffold					525:532	a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold	456:532	a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold	456:532	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	2	1	theme	casting	373:379	arg1	technologies					330:341	technologies	330:341	technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding	330:443	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	2	2	theme	/ethyl	481:486	arg1	scaffold					525:532	a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold	456:532	a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold	456:532	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	1	3	from	challenge	144:152	arg1	engineering					169:179	bone tissue engineering	157:179	bone tissue engineering	157:179	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	2	4	theme	solvent	365:371	arg1	casting					373:379	high concentration solvent casting	346:379	high concentration solvent casting	346:379	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	1	5	theme	mechanical	257:266	arg1	strength					268:275	mechanical strength	257:275	mechanical strength	257:275	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	2	6	dep	casting	373:379	arg1	molding					437:443	compression molding	425:443	compression molding	425:443	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	4	7	theme	porous	771:776	arg1	structure					778:786	desired porous structure	763:786	desired porous structure	763:786	The results indicated that the PLA/EC/HA scaffolds at the 20wt% HA loading level showed optimal mechanical properties and desired porous structure.
29103485	1	8	theme	strength	268:275	arg1	requirements					296:307	mechanical strength and pore structure requirements	257:307	mechanical strength and pore structure requirements	257:307	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	6	9	contain	have	1052:1055	arg2	potential					1057:1065	potential	1057:1065	potential to be used as a bone substitute material for bone tissue engineering application	1057:1146	Thus, the developed scaffolds have potential to be used as a bone substitute material for bone tissue engineering application.
29103485	6	9	contain	have	1052:1055	arg1	scaffolds					1042:1050	the developed scaffolds	1028:1050	the developed scaffolds	1028:1050	Thus, the developed scaffolds have potential to be used as a bone substitute material for bone tissue engineering application.
29103485	4	10	theme	mechanical	737:746	arg1	properties					748:757	optimal mechanical properties	729:757	optimal mechanical properties	729:757	The results indicated that the PLA/EC/HA scaffolds at the 20wt% HA loading level showed optimal mechanical properties and desired porous structure.
29103485	4	11	theme	optimal	729:735	arg1	properties					748:757	optimal mechanical properties	729:757	optimal mechanical properties	729:757	The results indicated that the PLA/EC/HA scaffolds at the 20wt% HA loading level showed optimal mechanical properties and desired porous structure.
29103485	5	12	theme	yield	830:834	arg1	strength					836:843	compressive yield strength	818:843	compressive yield strength	818:843	Its porosity, contact angle, compressive yield strength and weight loss after 56days were 84.28±7.04%, 45.13±2.40°, 1.57±0.09MPa and 4.77±0.32%, respectively, which could satisfy the physiological demands to guide bone regeneration.
29103485	3	13	theme	functional	539:548	arg1	properties					577:586	The functional, structural and mechanical properties	535:586	The functional, structural and mechanical properties of the obtained porous scaffolds	535:619	The functional, structural and mechanical properties of the obtained porous scaffolds were characterized.
29103485	1	14	theme	bone	157:160	arg1	engineering					169:179	bone tissue engineering	157:179	bone tissue engineering	157:179	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	0	15	theme	combined	97:104	arg1	method					106:111	a combined method	95:111	a combined method for bone regeneration	95:133	Porous stable poly(lactic acid)/ethyl cellulose/hydroxyapatite composite scaffolds prepared by a combined method for bone regeneration.
29103485	2	16	theme	novel	458:462	arg1	scaffold					525:532	a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold	456:532	a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold	456:532	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	1	17	theme	tissue	162:167	arg1	engineering					169:179	bone tissue engineering	157:179	bone tissue engineering	157:179	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	2	18	theme	poly	464:467	arg1	scaffold					525:532	a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold	456:532	a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold	456:532	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	6	19	theme	bone	1083:1086	arg1	material					1099:1106	a bone substitute material	1081:1106	a bone substitute material for bone tissue engineering application	1081:1146	Thus, the developed scaffolds have potential to be used as a bone substitute material for bone tissue engineering application.
29103485	2	20	theme	PLA/EC/HA	514:522	arg1	scaffold					525:532	a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold	456:532	a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold	456:532	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	4	21	theme	desired	763:769	arg1	structure					778:786	desired porous structure	763:786	desired porous structure	763:786	The results indicated that the PLA/EC/HA scaffolds at the 20wt% HA loading level showed optimal mechanical properties and desired porous structure.
29103485	4	22	theme	20wt	699:702	arg1	level					716:720	the 20wt% HA loading level	695:720	the 20wt% HA loading level	695:720	The results indicated that the PLA/EC/HA scaffolds at the 20wt% HA loading level showed optimal mechanical properties and desired porous structure.
29103485	0	23	theme	Porous	0:5	arg1	/ethyl					31:36	Porous stable poly(lactic acid)/ethyl	0:36	Porous stable poly(lactic acid)/ethyl	0:36	Porous stable poly(lactic acid)/ethyl cellulose/hydroxyapatite composite scaffolds prepared by a combined method for bone regeneration.
29103485	1	24	theme	pore	281:284	arg1	structure					286:294	pore structure	281:294	pore structure	281:294	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	2	25	theme	leaching	394:401	arg1	technologies					330:341	technologies	330:341	technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding	330:443	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	1	26	theme	structure	286:294	arg1	requirements					296:307	mechanical strength and pore structure requirements	257:307	mechanical strength and pore structure requirements	257:307	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	0	27	theme	bone	117:120	arg1	regeneration					122:133	bone regeneration	117:133	bone regeneration	117:133	Porous stable poly(lactic acid)/ethyl cellulose/hydroxyapatite composite scaffolds prepared by a combined method for bone regeneration.
29103485	3	28	theme	mechanical	566:575	arg1	properties					577:586	The functional, structural and mechanical properties	535:586	The functional, structural and mechanical properties of the obtained porous scaffolds	535:619	The functional, structural and mechanical properties of the obtained porous scaffolds were characterized.
29103485	6	29	dep	material	1099:1106	arg1	substitute					1088:1097	substitute	1088:1097	substitute	1088:1097	Thus, the developed scaffolds have potential to be used as a bone substitute material for bone tissue engineering application.
29103485	5	30	theme	physiological	972:984	arg1	demands					986:992	the physiological demands	968:992	the physiological demands to guide bone regeneration	968:1019	Its porosity, contact angle, compressive yield strength and weight loss after 56days were 84.28±7.04%, 45.13±2.40°, 1.57±0.09MPa and 4.77±0.32%, respectively, which could satisfy the physiological demands to guide bone regeneration.
29103485	0	31	theme	poly	14:17	arg1	/ethyl					31:36	Porous stable poly(lactic acid)/ethyl	0:36	Porous stable poly(lactic acid)/ethyl	0:36	Porous stable poly(lactic acid)/ethyl cellulose/hydroxyapatite composite scaffolds prepared by a combined method for bone regeneration.
29103485	4	32	theme	loading	708:714	arg1	level					716:720	the 20wt% HA loading level	695:720	the 20wt% HA loading level	695:720	The results indicated that the PLA/EC/HA scaffolds at the 20wt% HA loading level showed optimal mechanical properties and desired porous structure.
29103485	4	33	theme	HA	705:706	arg1	level					716:720	the 20wt% HA loading level	695:720	the 20wt% HA loading level	695:720	The results indicated that the PLA/EC/HA scaffolds at the 20wt% HA loading level showed optimal mechanical properties and desired porous structure.
29103485	5	34	theme	contact	803:809	arg1	angle					811:815	contact angle	803:815	contact angle	803:815	Its porosity, contact angle, compressive yield strength and weight loss after 56days were 84.28±7.04%, 45.13±2.40°, 1.57±0.09MPa and 4.77±0.32%, respectively, which could satisfy the physiological demands to guide bone regeneration.
29103485	4	35	theme	PLA/EC/HA	672:680	arg1	scaffolds					682:690	the PLA/EC/HA scaffolds	668:690	the PLA/EC/HA scaffolds at the 20wt% HA loading level	668:720	The results indicated that the PLA/EC/HA scaffolds at the 20wt% HA loading level showed optimal mechanical properties and desired porous structure.
29103485	3	36	theme	scaffolds	611:619	arg1	properties					577:586	The functional, structural and mechanical properties	535:586	The functional, structural and mechanical properties of the obtained porous scaffolds	535:619	The functional, structural and mechanical properties of the obtained porous scaffolds were characterized.
29103485	2	37	theme	compression	425:435	arg1	molding					437:443	compression molding	425:443	compression molding	425:443	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	0	38	theme	lactic	19:24	arg1	poly					14:17	stable poly	7:17	Porous stable poly(lactic acid)/ethyl	0:36	Porous stable poly(lactic acid)/ethyl cellulose/hydroxyapatite composite scaffolds prepared by a combined method for bone regeneration.
29103485	0	38	theme	lactic	19:24	arg1	acid					26:29	lactic acid	19:29	lactic acid	19:29	Porous stable poly(lactic acid)/ethyl cellulose/hydroxyapatite composite scaffolds prepared by a combined method for bone regeneration.
29103485	1	39	theme	biomimetic	203:212	arg1	scaffolds					214:222	biomimetic scaffolds	203:222	biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements	203:307	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	2	40	theme	lactic	469:474	arg1	poly					464:467	poly	464:467	a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold	456:532	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	2	40	theme	lactic	469:474	arg1	acid					476:479	lactic acid	469:479	lactic acid	469:479	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	3	41	theme	obtained	595:602	arg1	scaffolds					611:619	the obtained porous scaffolds	591:619	the obtained porous scaffolds	591:619	The functional, structural and mechanical properties of the obtained porous scaffolds were characterized.
29103485	6	42	theme	bone	1112:1115	arg1	engineering					1124:1134	bone tissue engineering	1112:1134	bone tissue engineering application	1112:1146	Thus, the developed scaffolds have potential to be used as a bone substitute material for bone tissue engineering application.
29103485	2	43	theme	concentration	351:363	arg1	casting					373:379	high concentration solvent casting	346:379	high concentration solvent casting	346:379	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	5	44	theme	compressive	818:828	arg1	strength					836:843	compressive yield strength	818:843	compressive yield strength	818:843	Its porosity, contact angle, compressive yield strength and weight loss after 56days were 84.28±7.04%, 45.13±2.40°, 1.57±0.09MPa and 4.77±0.32%, respectively, which could satisfy the physiological demands to guide bone regeneration.
29103485	2	45	theme	room	408:411	arg1	temperature					413:423	room temperature	408:423	room temperature	408:423	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	3	46	theme	porous	604:609	arg1	scaffolds					611:619	the obtained porous scaffolds	591:619	the obtained porous scaffolds	591:619	The functional, structural and mechanical properties of the obtained porous scaffolds were characterized.
29103485	2	47	theme	high	346:349	arg1	casting					373:379	high concentration solvent casting	346:379	high concentration solvent casting	346:379	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	5	48	theme	bone	1003:1006	arg1	regeneration					1008:1019	bone regeneration	1003:1019	bone regeneration	1003:1019	Its porosity, contact angle, compressive yield strength and weight loss after 56days were 84.28±7.04%, 45.13±2.40°, 1.57±0.09MPa and 4.77±0.32%, respectively, which could satisfy the physiological demands to guide bone regeneration.
29103485	4	49	theme	%	703:703	arg1	level					716:720	the 20wt% HA loading level	695:720	the 20wt% HA loading level	695:720	The results indicated that the PLA/EC/HA scaffolds at the 20wt% HA loading level showed optimal mechanical properties and desired porous structure.
29103485	6	50	theme	engineering	1124:1134	arg1	application					1136:1146	bone tissue engineering application	1112:1146	bone tissue engineering application	1112:1146	Thus, the developed scaffolds have potential to be used as a bone substitute material for bone tissue engineering application.
29103485	6	51	theme	tissue	1117:1122	arg1	engineering					1124:1134	bone tissue engineering	1112:1134	bone tissue engineering application	1112:1146	Thus, the developed scaffolds have potential to be used as a bone substitute material for bone tissue engineering application.
29103485	1	52	theme	scaffolds	214:222	arg1	development					188:198	the development	184:198	the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements	184:307	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	1	52	theme	scaffolds	214:222	arg1	challenge					144:152	A major challenge	136:152	A major challenge in bone tissue engineering	136:179	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	3	53	theme	structural	551:560	arg1	properties					577:586	The functional, structural and mechanical properties	535:586	The functional, structural and mechanical properties of the obtained porous scaffolds	535:619	The functional, structural and mechanical properties of the obtained porous scaffolds were characterized.
29103485	1	54	theme	major	138:142	arg1	development					188:198	the development	184:198	the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements	184:307	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	1	54	theme	major	138:142	arg1	challenge					144:152	A major challenge	136:152	A major challenge in bone tissue engineering	136:179	A major challenge in bone tissue engineering is the development of biomimetic scaffolds which should simultaneously meet mechanical strength and pore structure requirements.
29103485	6	55	theme	developed	1032:1040	arg1	scaffolds					1042:1050	the developed scaffolds	1028:1050	the developed scaffolds	1028:1050	Thus, the developed scaffolds have potential to be used as a bone substitute material for bone tissue engineering application.
29103485	0	56	theme	composite	63:71	arg1	scaffolds					73:81	composite scaffolds	63:81	composite scaffolds prepared by a combined method for bone regeneration	63:133	Porous stable poly(lactic acid)/ethyl cellulose/hydroxyapatite composite scaffolds prepared by a combined method for bone regeneration.
29103485	2	57	theme	temperature	413:423	arg1	technologies					330:341	technologies	330:341	technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding	330:443	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	2	58	theme	particulate	382:392	arg1	leaching					394:401	particulate leaching	382:401	particulate leaching	382:401	Herein, we combined technologies of high concentration solvent casting, particulate leaching, and room temperature compression molding to prepare a novel poly(lactic acid)/ethyl cellulose/hydroxyapatite (PLA/EC/HA) scaffold.
29103485	5	59	theme	weight	849:854	arg1	loss					856:859	weight loss	849:859	weight loss	849:859	Its porosity, contact angle, compressive yield strength and weight loss after 56days were 84.28±7.04%, 45.13±2.40°, 1.57±0.09MPa and 4.77±0.32%, respectively, which could satisfy the physiological demands to guide bone regeneration.
29103485	0	60	theme	stable	7:12	arg1	poly					14:17	stable poly	7:17	Porous stable poly(lactic acid)/ethyl	0:36	Porous stable poly(lactic acid)/ethyl cellulose/hydroxyapatite composite scaffolds prepared by a combined method for bone regeneration.
29103485	0	60	theme	stable	7:12	arg1	acid					26:29	lactic acid	19:29	lactic acid	19:29	Porous stable poly(lactic acid)/ethyl cellulose/hydroxyapatite composite scaffolds prepared by a combined method for bone regeneration.
29103485	4	61	from	level	716:720	arg1	scaffolds					682:690	the PLA/EC/HA scaffolds	668:690	the PLA/EC/HA scaffolds at the 20wt% HA loading level	668:720	The results indicated that the PLA/EC/HA scaffolds at the 20wt% HA loading level showed optimal mechanical properties and desired porous structure.
27896089	0	0	theme	recessive	77:85	arg1	laxa					93:96	an autosomal recessive cutis laxa	64:96	an autosomal recessive cutis laxa syndrome type IIA	64:114	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	4	1	theme	abnormal	597:604	arg1	glycosylation					629:641	abnormal transferrin (N-linked) glycosylation	597:641	abnormal transferrin (N-linked) glycosylation	597:641	Both patients presented abnormal transferrin (N-linked) glycosylation but Patient 1 had a normal ApoCIII (O-linked) glycosylation profile.
27896089	6	2	theme	RNA	1043:1045	arg1	processing					1047:1056	RNA processing	1043:1056	RNA processing	1043:1056	In Patient 2 we found a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10).
27896089	2	3	theme	laxa	376:379	arg1	syndromes					381:389	other cutis laxa syndromes	364:389	other cutis laxa syndromes	364:389	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	0	4	theme	autosomal	67:75	arg1	laxa					93:96	an autosomal recessive cutis laxa	64:96	an autosomal recessive cutis laxa syndrome type IIA	64:114	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	1	5	from	mutations	147:155	arg1	ATP6V0A2					160:167	ATP6V0A2	160:167	ATP6V0A2	160:167	Patients with ARCL-IIA harbor mutations in ATP6V0A2 that codes for an organelle proton pump.
27896089	2	6	theme	cutis	370:374	arg1	syndromes					381:389	other cutis laxa syndromes	364:389	other cutis laxa syndromes	364:389	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	4	7	theme	transferrin	606:616	arg1	glycosylation					629:641	abnormal transferrin (N-linked) glycosylation	597:641	abnormal transferrin (N-linked) glycosylation	597:641	Both patients presented abnormal transferrin (N-linked) glycosylation but Patient 1 had a normal ApoCIII (O-linked) glycosylation profile.
27896089	0	8	theme	laxa	93:96	arg1	syndrome					98:105	an autosomal recessive cutis laxa syndrome type IIA	64:114	an autosomal recessive cutis laxa syndrome type IIA	64:114	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	7	9	theme	world	1356:1360	arg1	regions					1341:1347	different regions	1331:1347	different regions of the world	1331:1360	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	2	10	theme	N-linked	302:309	arg1	glycosylations					324:337	N-linked and O-linked glycosylations	302:337	N-linked and O-linked glycosylations	302:337	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	0	11	theme	cutis	87:91	arg1	laxa					93:96	an autosomal recessive cutis laxa	64:96	an autosomal recessive cutis laxa syndrome type IIA	64:114	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	7	12	dep	report	1164:1169	arg1	establish					1257:1265	establish	1257:1265	to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds	1254:1399	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	7	12	dep	report	1164:1169	arg1	describe					1174:1181	describe	1174:1181	to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG	1171:1248	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	0	13	attach	present	19:25	arg2	mutations					9:17	ATP6V0A2 mutations	0:17	ATP6V0A2 mutations	0:17	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	0	13	attach	present	19:25	arg1	children					50:57	two Mexican Mestizo children	30:57	two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA	30:114	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	0	14	dep	syndrome	98:105	arg1	IIA					112:114	type IIA	107:114	an autosomal recessive cutis laxa syndrome type IIA	64:114	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	4	15	theme	N-linked	619:626	arg1	glycosylation					629:641	abnormal transferrin (N-linked) glycosylation	597:641	abnormal transferrin (N-linked) glycosylation	597:641	Both patients presented abnormal transferrin (N-linked) glycosylation but Patient 1 had a normal ApoCIII (O-linked) glycosylation profile.
27896089	0	16	theme	type	107:110	arg1	IIA					112:114	type IIA	107:114	an autosomal recessive cutis laxa syndrome type IIA	64:114	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	1	17	with	Patients	117:124	arg1	ARCL-IIA					131:138	ARCL-IIA	131:138	ARCL-IIA	131:138	Patients with ARCL-IIA harbor mutations in ATP6V0A2 that codes for an organelle proton pump.
27896089	3	18	theme	Mexican	483:489	arg1	patients					499:506	two Mexican Mestizo patients	479:506	two Mexican Mestizo patients with a clinical phenotype corresponding to an ARCL-IIA syndrome	479:570	We studied two Mexican Mestizo patients with a clinical phenotype corresponding to an ARCL-IIA syndrome.
27896089	7	19	from	regions	1341:1347	arg1	patients					1317:1324	patients	1317:1324	patients from different regions of the world and with different genetic backgrounds	1317:1399	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	2	20	dep	presents	251:258	arg1	differentiating					340:354	differentiating	340:354	differentiating it from other cutis laxa syndromes	340:389	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	2	20	dep	presents	251:258	arg1	classifying					395:405	classifying	395:405	classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG)	395:465	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	5	21	theme	nonsense	827:834	arg1	mutation					836:843	a previously reported homozygous nonsense mutation c.187C>T	794:852	a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD	794:911	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
27896089	5	21	theme	nonsense	827:834	arg1	p.R63X					855:860	p.R63X	855:860	p.R63X	855:860	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
27896089	3	22	with	patients	499:506	arg1	phenotype					524:532	a clinical phenotype	513:532	a clinical phenotype corresponding to an ARCL-IIA syndrome	513:570	We studied two Mexican Mestizo patients with a clinical phenotype corresponding to an ARCL-IIA syndrome.
27896089	7	23	theme	Mestizo	1183:1189	arg1	patients					1191:1198	Mestizo patients	1183:1198	Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG	1183:1248	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	2	24	theme	glycosylation	271:283	arg1	defect					285:290	a combined glycosylation defect	260:290	a combined glycosylation defect affecting N-linked and O-linked glycosylations	260:337	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	5	25	theme	clinical	887:894	arg1	finding					896:902	a novel clinical finding	879:902	a novel clinical finding of a VSD	879:911	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
27896089	7	26	theme	first	1158:1162	arg1	This					1146:1149	This	1146:1149	This	1146:1149	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	7	26	theme	first	1158:1162	arg1	report					1164:1169	the first report	1154:1169	the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds	1154:1399	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	7	27	theme	molecular	1205:1213	arg1	diagnosis					1215:1223	molecular diagnosis	1205:1223	molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG	1205:1248	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	4	28	theme	glycosylation	689:701	arg1	profile					703:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	Both patients presented abnormal transferrin (N-linked) glycosylation but Patient 1 had a normal ApoCIII (O-linked) glycosylation profile.
27896089	0	29	theme	ATP6V0A2	0:7	arg1	mutations					9:17	ATP6V0A2 mutations	0:17	ATP6V0A2 mutations	0:17	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	7	30	located	found	1308:1312	arg2	mutations					1278:1286	their mutations	1272:1286	their mutations	1272:1286	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	7	30	located	found	1308:1312	arg1	patients					1317:1324	patients	1317:1324	patients from different regions of the world and with different genetic backgrounds	1317:1399	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	7	30	located	found	1308:1312	arg2	first					1296:1300	first	1296:1300	first	1296:1300	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	5	31	theme	novel	881:885	arg1	finding					896:902	a novel clinical finding	879:902	a novel clinical finding of a VSD	879:911	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
27896089	7	32	with	patients	1191:1198	arg1	diagnosis					1215:1223	molecular diagnosis	1205:1223	molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG	1205:1248	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	2	33	theme	O-linked	315:322	arg1	glycosylations					324:337	N-linked and O-linked glycosylations	302:337	N-linked and O-linked glycosylations	302:337	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	2	34	theme	Glycosylation	438:450	arg1	Disorder					426:433	a Congenital Disorder	413:433	a Congenital Disorder of Glycosylation (ATP6V0A2-CDG)	413:465	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	2	34	theme	Glycosylation	438:450	arg1	it					407:408	it	407:408	it	407:408	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	2	35	link	N-linked	302:309	arg1	glycosylations					324:337	N-linked and O-linked glycosylations	302:337	N-linked and O-linked glycosylations	302:337	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	5	36	theme	ATP6V0A2	736:743	arg1	screening					723:731	Mutational screening	712:731	Mutational screening of ATP6V0A2 using cDNA and genomic DNA	712:770	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
27896089	7	37	theme	ARCL-IIA/ATP6V0A2-CDG	1228:1248	arg1	diagnosis					1215:1223	molecular diagnosis	1205:1223	molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG	1205:1248	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	1	38	theme	organelle	187:195	arg1	pump					204:207	an organelle proton pump	184:207	an organelle proton pump	184:207	Patients with ARCL-IIA harbor mutations in ATP6V0A2 that codes for an organelle proton pump.
27896089	0	39	theme	Mexican	34:40	arg1	children					50:57	two Mexican Mestizo children	30:57	two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA	30:114	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	6	40	theme	p.Q765X	960:966	arg1	mutation					969:976	a homozygous c.2293C>T (p.Q765X) mutation	936:976	a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10)	936:1143	In Patient 2 we found a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10).
27896089	4	41	theme	ApoCIII	670:676	arg1	profile					703:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	Both patients presented abnormal transferrin (N-linked) glycosylation but Patient 1 had a normal ApoCIII (O-linked) glycosylation profile.
27896089	1	42	theme	proton	197:202	arg1	pump					204:207	an organelle proton pump	184:207	an organelle proton pump	184:207	Patients with ARCL-IIA harbor mutations in ATP6V0A2 that codes for an organelle proton pump.
27896089	4	43	theme	O-linked	679:686	arg1	profile					703:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	Both patients presented abnormal transferrin (N-linked) glycosylation but Patient 1 had a normal ApoCIII (O-linked) glycosylation profile.
27896089	2	44	link	O-linked	315:322	arg1	glycosylations					324:337	N-linked and O-linked glycosylations	302:337	N-linked and O-linked glycosylations	302:337	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	5	45	theme	reported	807:814	arg1	mutation					836:843	a previously reported homozygous nonsense mutation c.187C>T	794:852	a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD	794:911	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
27896089	5	45	theme	reported	807:814	arg1	p.R63X					855:860	p.R63X	855:860	p.R63X	855:860	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
27896089	4	46	contain	had	657:659	arg2	profile					703:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	Both patients presented abnormal transferrin (N-linked) glycosylation but Patient 1 had a normal ApoCIII (O-linked) glycosylation profile.
27896089	4	46	contain	had	657:659	arg1	Patient					647:653	Patient 1	647:655	Patient 1	647:655	Both patients presented abnormal transferrin (N-linked) glycosylation but Patient 1 had a normal ApoCIII (O-linked) glycosylation profile.
27896089	2	47	theme	ARCL-IIA	214:221	arg1	syndrome					223:230	The ARCL-IIA syndrome	210:230	The ARCL-IIA syndrome	210:230	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	2	48	theme	Congenital	415:424	arg1	Disorder					426:433	a Congenital Disorder	413:433	a Congenital Disorder of Glycosylation (ATP6V0A2-CDG)	413:465	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	2	48	theme	Congenital	415:424	arg1	it					407:408	it	407:408	it	407:408	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	5	49	theme	homozygous	816:825	arg1	mutation					836:843	a previously reported homozygous nonsense mutation c.187C>T	794:852	a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD	794:911	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
27896089	5	49	theme	homozygous	816:825	arg1	p.R63X					855:860	p.R63X	855:860	p.R63X	855:860	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
27896089	3	50	theme	clinical	515:522	arg1	phenotype					524:532	a clinical phenotype	513:532	a clinical phenotype corresponding to an ARCL-IIA syndrome	513:570	We studied two Mexican Mestizo patients with a clinical phenotype corresponding to an ARCL-IIA syndrome.
27896089	4	51	link	O-linked	679:686	arg1	profile					703:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	Both patients presented abnormal transferrin (N-linked) glycosylation but Patient 1 had a normal ApoCIII (O-linked) glycosylation profile.
27896089	7	52	with	patients	1317:1324	arg1	backgrounds					1389:1399	different genetic backgrounds	1371:1399	different genetic backgrounds	1371:1399	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	2	53	theme	other	364:368	arg1	syndromes					381:389	other cutis laxa syndromes	364:389	other cutis laxa syndromes	364:389	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	0	54	theme	Mestizo	42:48	arg1	children					50:57	two Mexican Mestizo children	30:57	two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA	30:114	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	6	55	theme	homozygous	938:947	arg1	mutation					969:976	a homozygous c.2293C>T (p.Q765X) mutation	936:976	a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10)	936:1143	In Patient 2 we found a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10).
27896089	6	56	dep	identified	1103:1112	arg1	r.2176_2293del					1115:1128	r.2176_2293del	1115:1128	r.2176_2293del; p.F726Sfs*10	1115:1142	In Patient 2 we found a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10).
27896089	6	56	dep	identified	1103:1112	arg1	transcript					1077:1086	a novel transcript	1069:1086	a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10)	1069:1143	In Patient 2 we found a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10).
27896089	6	57	theme	p.F726Sfs	1131:1139	arg1	*					1140:1140	p.F726Sfs*10	1131:1142	r.2176_2293del; p.F726Sfs*10	1115:1142	In Patient 2 we found a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10).
27896089	5	58	theme	Mutational	712:721	arg1	screening					723:731	Mutational screening	712:731	Mutational screening of ATP6V0A2 using cDNA and genomic DNA	712:770	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
27896089	4	59	link	N-linked	619:626	arg1	glycosylation					629:641	abnormal transferrin (N-linked) glycosylation	597:641	abnormal transferrin (N-linked) glycosylation	597:641	Both patients presented abnormal transferrin (N-linked) glycosylation but Patient 1 had a normal ApoCIII (O-linked) glycosylation profile.
27896089	2	60	theme	combined	262:269	arg1	defect					285:290	a combined glycosylation defect	260:290	a combined glycosylation defect affecting N-linked and O-linked glycosylations	260:337	The ARCL-IIA syndrome characteristically presents a combined glycosylation defect affecting N-linked and O-linked glycosylations, differentiating it from other cutis laxa syndromes and classifying it as a Congenital Disorder of Glycosylation (ATP6V0A2-CDG).
27896089	7	61	theme	different	1371:1379	arg1	backgrounds					1389:1399	different genetic backgrounds	1371:1399	different genetic backgrounds	1371:1399	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	3	62	theme	Mestizo	491:497	arg1	patients					499:506	two Mexican Mestizo patients	479:506	two Mexican Mestizo patients with a clinical phenotype corresponding to an ARCL-IIA syndrome	479:570	We studied two Mexican Mestizo patients with a clinical phenotype corresponding to an ARCL-IIA syndrome.
27896089	7	63	theme	genetic	1381:1387	arg1	backgrounds					1389:1399	different genetic backgrounds	1371:1399	different genetic backgrounds	1371:1399	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	3	64	theme	ARCL-IIA	554:561	arg1	syndrome					563:570	an ARCL-IIA syndrome	551:570	an ARCL-IIA syndrome	551:570	We studied two Mexican Mestizo patients with a clinical phenotype corresponding to an ARCL-IIA syndrome.
27896089	6	65	dep	r.2176_2293del	1115:1128	arg1	*					1140:1140	p.F726Sfs*10	1131:1142	r.2176_2293del; p.F726Sfs*10	1115:1142	In Patient 2 we found a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10).
27896089	0	66	with	children	50:57	arg1	syndrome					98:105	an autosomal recessive cutis laxa syndrome type IIA	64:114	an autosomal recessive cutis laxa syndrome type IIA	64:114	ATP6V0A2 mutations present in two Mexican Mestizo children with an autosomal recessive cutis laxa syndrome type IIA.
27896089	4	67	theme	normal	663:668	arg1	profile					703:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	a normal ApoCIII (O-linked) glycosylation profile	661:709	Both patients presented abnormal transferrin (N-linked) glycosylation but Patient 1 had a normal ApoCIII (O-linked) glycosylation profile.
27896089	7	68	theme	different	1331:1339	arg1	regions					1341:1347	different regions	1331:1347	different regions of the world	1331:1360	This is the first report to describe Mestizo patients with molecular diagnosis of ARCL-IIA/ATP6V0A2-CDG and to establish that their mutations are the first to be found in patients from different regions of the world and with different genetic backgrounds.
27896089	6	69	theme	novel	1071:1075	arg1	r.2176_2293del					1115:1128	r.2176_2293del	1115:1128	r.2176_2293del; p.F726Sfs*10	1115:1142	In Patient 2 we found a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10).
27896089	6	69	theme	novel	1071:1075	arg1	transcript					1077:1086	a novel transcript	1069:1086	a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10)	1069:1143	In Patient 2 we found a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10).
27896089	5	70	theme	VSD	909:911	arg1	finding					896:902	a novel clinical finding	879:902	a novel clinical finding of a VSD	879:911	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
27896089	6	71	theme	c.2293C>T	949:957	arg1	mutation					969:976	a homozygous c.2293C>T (p.Q765X) mutation	936:976	a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10)	936:1143	In Patient 2 we found a homozygous c.2293C>T (p.Q765X) mutation that had been previously reported but found that it also altered RNA processing generating a novel transcript not previously identified (r.2176_2293del; p.F726Sfs*10).
27896089	5	72	theme	genomic	760:766	arg1	DNA					768:770	genomic DNA	760:770	genomic DNA	760:770	Mutational screening of ATP6V0A2 using cDNA and genomic DNA revealed in Patient 1 a previously reported homozygous nonsense mutation c.187C>T (p.R63X) associated with a novel clinical finding of a VSD.
28822541	6	0	dep	treatments	1105:1114	arg1	MR					1203:1204	a low-lactose MR	1189:1204	a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio)	1189:1309	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	0	dep	treatments	1105:1114	arg1	treatments					1105:1114	2 MR treatments	1100:1114	2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio)	1100:1309	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	0	dep	treatments	1105:1114	arg1	MR					1127:1128	a control MR	1117:1128	a control MR that contained lactose as the only carbohydrate source	1117:1183	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	7	1	from	Relations	1312:1320	arg1	life					1389:1392	later life	1383:1392	later life	1383:1392	Relations between early life characteristics and growth performance in later life were assessed in 117 clinically healthy calves.
28822541	15	2	theme	fecal	2498:2502	arg1	pH					2504:2505	fecal pH	2498:2505	fecal pH	2498:2505	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	0	3	from	metabolism	125:134	arg1	life					180:183	early life	174:183	early life	174:183	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	6	4	theme	control	1119:1125	arg1	treatments					1105:1114	2 MR treatments	1100:1114	2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio)	1100:1309	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	4	theme	control	1119:1125	arg1	MR					1127:1128	a control MR	1117:1128	a control MR that contained lactose as the only carbohydrate source	1117:1183	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	15	5	theme	plasma	2467:2472	arg1	concentrations					2482:2495	fasting plasma glucose concentrations	2459:2495	fasting plasma glucose concentrations	2459:2495	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	4	6	theme	first	783:787	arg1	feeding					789:795	the first feeding	779:795	the first feeding	779:795	A total of 180 male Holstein-Friesian calves arrived at the facilities at 17 ± 3.4 d of age, and blood samples were collected before the first feeding.
28822541	0	7	from	digestion	114:122	arg1	life					180:183	early life	174:183	early life	174:183	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	13	8	theme	later	2222:2226	arg1	life					2228:2231	later life	2222:2231	later life	2222:2231	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	4	9	theme	blood	743:747	arg1	samples					749:755	blood samples	743:755	blood samples	743:755	A total of 180 male Holstein-Friesian calves arrived at the facilities at 17 ± 3.4 d of age, and blood samples were collected before the first feeding.
28822541	10	10	theme	variation	1867:1875	arg1	variation					1867:1875	variation	1867:1875	variation	1867:1875	Variation in observations in period 1 predicted 17% of variation in ADG in period 2.
28822541	10	10	theme	variation	1867:1875	arg1	%					1862:1862	17%	1860:1862	17% of variation	1860:1875	Variation in observations in period 1 predicted 17% of variation in ADG in period 2.
28822541	0	11	theme	behavioral	153:162	arg1	traits					164:169	behavioral traits	153:169	behavioral traits	153:169	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	8	12	theme	control	1504:1510	arg1	calves					1512:1517	control calves	1504:1517	control calves	1504:1517	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	16	13	theme	early	2747:2751	arg1	life					2753:2756	early life	2747:2756	early life on their ability to cope with MR varying in lactose content	2747:2816	These measurements are therefore potential tools for screening calves in early life on their ability to cope with MR varying in lactose content.
28822541	15	14	theme	natural	2535:2541	arg1	antibodies					2543:2552	plasma natural antibodies	2528:2552	plasma natural antibodies	2528:2552	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	9	15	theme	component	1670:1678	arg1	analysis					1680:1687	principal component analysis	1660:1687	principal component analysis	1660:1687	Observations in period 1 were clustered per category using principal component analysis, and the resulting principal components were used to predict performance in period 2 using multiple regression procedures.
28822541	12	16	from	ADG	2058:2060	arg1	%					2023:2023	only 7%	2017:2023	only 7% of the variation in standardized ADG in period 2	2017:2072	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	12	16	from	ADG	2058:2060	arg1	period					2065:2070	period 2	2065:2072	period 2	2065:2072	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	12	16	from	ADG	2058:2060	arg1	variation					2032:2040	the variation	2028:2040	the variation in standardized ADG in period 2	2028:2072	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	15	17	theme	glucose	2474:2480	arg1	concentrations					2482:2495	fasting plasma glucose concentrations	2459:2495	fasting plasma glucose concentrations	2459:2495	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	2	18	theme	calves	498:503	arg1	characterization					472:487	early life characterization	461:487	early life characterization of these calves	461:503	Our first objective was to examine whether variation in growth performance in healthy veal calves can be predicted from early life characterization of these calves.
28822541	12	19	theme	life	2139:2142	arg1	measurements					2144:2155	early life measurements	2133:2155	early life measurements	2133:2155	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	6	20	theme	carbohydrate	1165:1176	arg1	lactose					1145:1151	lactose	1145:1151	lactose	1145:1151	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	20	theme	carbohydrate	1165:1176	arg1	source					1178:1183	the only carbohydrate source	1156:1183	the only carbohydrate source	1156:1183	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	5	21	theme	feeding	944:950	arg1	categories					932:941	5 categories	930:941	5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology	930:1036	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	5	21	theme	feeding	944:950	arg1	motivation					952:961	feeding motivation	944:961	feeding motivation	944:961	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	8	22	theme	±	1526:1526	arg1	g/d					1532:1534	1,292 ± 111 g/d	1520:1534	1,292 ± 111 g/d	1520:1534	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	15	23	theme	lactose	2594:2600	arg1	replacer					2602:2609	the lactose replacer	2590:2609	the lactose replacer	2590:2609	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	15	24	theme	later	2635:2639	arg1	life					2641:2644	later life	2635:2644	later life	2635:2644	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	15	25	theme	drinking	2508:2515	arg1	speed					2517:2521	drinking speed	2508:2521	drinking speed	2508:2521	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	13	26	theme	early	2259:2263	arg1	characterization					2270:2285	early life characterization	2259:2285	early life characterization of the calves	2259:2299	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	0	27	from	efficiency	33:42	arg1	%					6:6	Only 7%	0:6	Only 7% of the variation in feed efficiency in veal calves	0:57	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	0	27	from	efficiency	33:42	arg1	variation					15:23	the variation	11:23	the variation in feed efficiency in veal calves	11:57	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	9	28	theme	principal	1708:1716	arg1	components					1718:1727	the resulting principal components	1694:1727	the resulting principal components	1694:1727	Observations in period 1 were clustered per category using principal component analysis, and the resulting principal components were used to predict performance in period 2 using multiple regression procedures.
28822541	13	29	from	efficiency	2208:2217	arg1	variation					2190:2198	the variation	2186:2198	the variation in feed efficiency in later life	2186:2231	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	13	29	from	efficiency	2208:2217	arg1	%					2181:2181	>90%	2178:2181	>90% of the variation in feed efficiency in later life	2178:2231	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	16	30	from	life	2753:2756	arg1	ability					2767:2773	their ability to cope with MR varying in lactose content	2761:2816	their ability to cope with MR varying in lactose content	2761:2816	These measurements are therefore potential tools for screening calves in early life on their ability to cope with MR varying in lactose content.
28822541	11	31	theme	solid	1946:1950	arg1	refusals					1957:1964	solid feed refusals	1946:1964	solid feed refusals	1946:1964	However, this was mainly related to variation in solid feed refusals.
28822541	15	32	from	speed	2517:2521	arg1	life					2563:2566	early life	2557:2566	early life (i.e., not exposed to the lactose replacer)	2557:2610	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	0	33	from	%	6:6	arg1	efficiency					33:42	feed efficiency	28:42	feed efficiency	28:42	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	0	33	from	%	6:6	arg1	calves					52:57	veal calves	47:57	veal calves	47:57	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	0	34	theme	veal	47:50	arg1	calves					52:57	veal calves	47:57	veal calves	47:57	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	16	35	theme	lactose	2802:2808	arg1	content					2810:2816	lactose content	2802:2816	lactose content	2802:2816	These measurements are therefore potential tools for screening calves in early life on their ability to cope with MR varying in lactose content.
28822541	15	36	theme	fasting	2459:2465	arg1	concentrations					2482:2495	fasting plasma glucose concentrations	2459:2495	fasting plasma glucose concentrations	2459:2495	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	7	37	theme	later	1383:1387	arg1	life					1389:1392	later life	1383:1392	later life	1383:1392	Relations between early life characteristics and growth performance in later life were assessed in 117 clinically healthy calves.
28822541	2	38	theme	life	467:470	arg1	characterization					472:487	early life characterization	461:487	early life characterization of these calves	461:503	Our first objective was to examine whether variation in growth performance in healthy veal calves can be predicted from early life characterization of these calves.
28822541	2	39	from	variation	384:392	arg1	performance					404:414	growth performance	397:414	growth performance in healthy veal calves	397:437	Our first objective was to examine whether variation in growth performance in healthy veal calves can be predicted from early life characterization of these calves.
28822541	6	40	theme	low-lactose	1191:1201	arg1	MR					1203:1204	a low-lactose MR	1189:1204	a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio)	1189:1309	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	40	theme	low-lactose	1191:1201	arg1	treatments					1105:1114	2 MR treatments	1100:1114	2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio)	1100:1309	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	1	41	theme	milk	309:312	arg1	composition					328:338	milk replacer (MR) composition	309:338	milk replacer (MR) composition	309:338	High interindividual variation in growth performance is commonly observed in veal calf production and appears to depend on milk replacer (MR) composition.
28822541	13	42	theme	calves	2294:2299	arg1	characterization					2270:2285	early life characterization	2259:2285	early life characterization of the calves	2259:2299	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	1	43	located	observed	251:258	arg1	production					273:282	veal calf production	263:282	veal calf production	263:282	High interindividual variation in growth performance is commonly observed in veal calf production and appears to depend on milk replacer (MR) composition.
28822541	1	43	located	observed	251:258	arg2	variation					207:215	High interindividual variation	186:215	High interindividual variation in growth performance	186:237	High interindividual variation in growth performance is commonly observed in veal calf production and appears to depend on milk replacer (MR) composition.
28822541	9	44	from	performance	1750:1760	arg1	period					1765:1770	period 2	1765:1772	period 2	1765:1772	Observations in period 1 were clustered per category using principal component analysis, and the resulting principal components were used to predict performance in period 2 using multiple regression procedures.
28822541	9	45	theme	regression	1789:1798	arg1	procedures					1800:1809	multiple regression procedures	1780:1809	multiple regression procedures	1780:1809	Observations in period 1 were clustered per category using principal component analysis, and the resulting principal components were used to predict performance in period 2 using multiple regression procedures.
28822541	4	46	theme	male	661:664	arg1	calves					684:689	180 male Holstein-Friesian calves	657:689	180 male Holstein-Friesian calves	657:689	A total of 180 male Holstein-Friesian calves arrived at the facilities at 17 ± 3.4 d of age, and blood samples were collected before the first feeding.
28822541	5	47	theme	following	845:853	arg1	period					861:866	period 1	861:868	period 1	861:868	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	5	47	theme	following	845:853	arg1	wk					857:858	the following 9 wk	841:858	the following 9 wk (period 1)	841:869	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	14	48	theme	feed	2398:2401	arg1	efficiency					2403:2412	feed efficiency	2398:2412	feed efficiency in later life	2398:2426	It is speculated that variation in health status explains a substantial portion of variation in feed efficiency in later life.
28822541	4	49	theme	calves	684:689	arg1	total					648:652	A total	646:652	A total of 180 male Holstein-Friesian calves	646:689	A total of 180 male Holstein-Friesian calves arrived at the facilities at 17 ± 3.4 d of age, and blood samples were collected before the first feeding.
28822541	13	50	theme	life	2265:2268	arg1	characterization					2270:2285	early life characterization	2259:2285	early life characterization of the calves	2259:2299	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	14	51	theme	variation	2385:2393	arg1	portion					2374:2380	a substantial portion	2360:2380	a substantial portion of variation	2360:2393	It is speculated that variation in health status explains a substantial portion of variation in feed efficiency in later life.
28822541	2	52	theme	healthy	419:425	arg1	calves					432:437	healthy veal calves	419:437	healthy veal calves	419:437	Our first objective was to examine whether variation in growth performance in healthy veal calves can be predicted from early life characterization of these calves.
28822541	15	53	from	relations	2441:2449	arg1	life					2641:2644	later life	2635:2644	later life	2635:2644	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	5	54	dep	categories	932:941	arg1	categories					932:941	5 categories	930:941	5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology	930:1036	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	5	54	dep	categories	932:941	arg1	metabolism					990:999	postabsorptive metabolism	975:999	postabsorptive metabolism	975:999	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	5	54	dep	categories	932:941	arg1	immunology					1027:1036	immunology	1027:1036	immunology	1027:1036	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	5	54	dep	categories	932:941	arg1	motivation					952:961	feeding motivation	944:961	feeding motivation	944:961	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	5	54	dep	categories	932:941	arg1	behavior					1002:1009	behavior	1002:1009	behavior	1002:1009	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	5	54	dep	categories	932:941	arg1	digestion					964:972	digestion	964:972	digestion	964:972	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	6	55	theme	lactose	1226:1232	arg1	lactose					1226:1232	the lactose	1222:1232	the lactose	1222:1232	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	55	theme	lactose	1226:1232	arg1	%					1217:1217	51%	1215:1217	51% of the lactose	1215:1232	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	1	56	theme	veal	263:266	arg1	production					273:282	veal calf production	263:282	veal calf production	263:282	High interindividual variation in growth performance is commonly observed in veal calf production and appears to depend on milk replacer (MR) composition.
28822541	12	57	from	%	2023:2023	arg1	ADG					2058:2060	standardized ADG	2045:2060	standardized ADG in period 2	2045:2072	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	7	58	theme	early	1330:1334	arg1	characteristics					1341:1355	early life characteristics	1330:1355	early life characteristics	1330:1355	Relations between early life characteristics and growth performance in later life were assessed in 117 clinically healthy calves.
28822541	12	59	theme	feed	2094:2097	arg1	efficiency					2099:2108	feed efficiency	2094:2108	feed efficiency	2094:2108	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	6	60	theme	MR	1102:1103	arg1	treatments					1105:1114	2 MR treatments	1100:1114	2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio)	1100:1309	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	60	theme	MR	1102:1103	arg1	MR					1203:1204	a low-lactose MR	1189:1204	a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio)	1189:1309	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	60	theme	MR	1102:1103	arg1	MR					1127:1128	a control MR	1117:1128	a control MR that contained lactose as the only carbohydrate source	1117:1183	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	3	61	theme	low-lactose	616:626	arg1	MR					628:629	a high- or low-lactose MR	605:629	a high- or low-lactose MR in later life	605:643	Our second objective was to determine whether these predictions differ between calves that are fed a high- or low-lactose MR in later life.
28822541	6	62	theme	2:1:2	1298:1302	arg1	glycerol					1288:1295	glycerol	1288:1295	glycerol (2:1:2 ratio)	1288:1309	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	62	theme	2:1:2	1298:1302	arg1	ratio					1304:1308	2:1:2 ratio	1298:1308	2:1:2 ratio	1298:1308	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	5	63	theme	targeted	877:884	arg1	challenges					886:895	targeted challenges	877:895	targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology	877:1036	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	16	64	theme	screening	2727:2735	arg1	calves					2737:2742	screening calves	2727:2742	screening calves in early life on their ability to cope with MR varying in lactose content	2727:2816	These measurements are therefore potential tools for screening calves in early life on their ability to cope with MR varying in lactose content.
28822541	3	65	theme	high-	607:611	arg1	MR					628:629	a high- or low-lactose MR	605:629	a high- or low-lactose MR in later life	605:643	Our second objective was to determine whether these predictions differ between calves that are fed a high- or low-lactose MR in later life.
28822541	4	66	theme	3.4	725:727	arg1	d					729:729	17 ± 3.4 d	720:729	17 ± 3.4 d of age	720:736	A total of 180 male Holstein-Friesian calves arrived at the facilities at 17 ± 3.4 d of age, and blood samples were collected before the first feeding.
28822541	1	67	theme	High	186:189	arg1	variation					207:215	High interindividual variation	186:215	High interindividual variation in growth performance	186:237	High interindividual variation in growth performance is commonly observed in veal calf production and appears to depend on milk replacer (MR) composition.
28822541	5	68	theme	related	897:903	arg1	challenges					886:895	targeted challenges	877:895	targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology	877:1036	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	15	69	from	pH	2504:2505	arg1	life					2563:2566	early life	2557:2566	early life (i.e., not exposed to the lactose replacer)	2557:2610	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	13	70	theme	feed	2203:2206	arg1	efficiency					2208:2217	feed efficiency	2203:2217	feed efficiency	2203:2217	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	12	71	theme	solid	1998:2002	arg1	intake					2009:2014	equal solid feed intake	1992:2014	equal solid feed intake	1992:2014	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	13	72	from	life	2228:2231	arg1	variation					2190:2198	the variation	2186:2198	the variation in feed efficiency in later life	2186:2231	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	13	72	from	life	2228:2231	arg1	%					2181:2181	>90%	2178:2181	>90% of the variation in feed efficiency in later life	2178:2231	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	13	73	theme	variation	2190:2198	arg1	variation					2190:2198	the variation	2186:2198	the variation in feed efficiency in later life	2186:2231	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	13	73	theme	variation	2190:2198	arg1	%					2181:2181	>90%	2178:2181	>90% of the variation in feed efficiency in later life	2178:2231	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	15	74	theme	MR	2658:2659	arg1	composition					2661:2671	MR composition	2658:2671	MR composition	2658:2671	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	1	75	theme	growth	220:225	arg1	performance					227:237	growth performance	220:237	growth performance	220:237	High interindividual variation in growth performance is commonly observed in veal calf production and appears to depend on milk replacer (MR) composition.
28822541	16	76	from	ability	2767:2773	arg1	calves					2737:2742	screening calves	2727:2742	screening calves in early life on their ability to cope with MR varying in lactose content	2727:2816	These measurements are therefore potential tools for screening calves in early life on their ability to cope with MR varying in lactose content.
28822541	8	77	theme	daily	1450:1454	arg1	ADG					1462:1464	ADG	1462:1464	ADG	1462:1464	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	8	77	theme	daily	1450:1454	arg1	gain					1456:1459	Average daily gain	1442:1459	Average daily gain (ADG) in period 2	1442:1477	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	15	78	theme	Significant	2429:2439	arg1	relations					2441:2449	Significant relations	2429:2449	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer)	2429:2610	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	16	79	theme	potential	2707:2715	arg1	measurements					2680:2691	These measurements	2674:2691	These measurements	2674:2691	These measurements are therefore potential tools for screening calves in early life on their ability to cope with MR varying in lactose content.
28822541	16	79	theme	potential	2707:2715	arg1	tools					2717:2721	potential tools	2707:2721	potential tools for screening calves in early life on their ability to cope with MR varying in lactose content	2707:2816	These measurements are therefore potential tools for screening calves in early life on their ability to cope with MR varying in lactose content.
28822541	15	80	theme	early	2557:2561	arg1	life					2563:2566	early life	2557:2566	early life (i.e., not exposed to the lactose replacer)	2557:2610	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	9	81	theme	principal	1660:1668	arg1	analysis					1680:1687	principal component analysis	1660:1687	principal component analysis	1660:1687	Observations in period 1 were clustered per category using principal component analysis, and the resulting principal components were used to predict performance in period 2 using multiple regression procedures.
28822541	0	82	theme	early	174:178	arg1	life					180:183	early life	174:183	early life	174:183	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	3	83	theme	second	510:515	arg1	objective					517:525	Our second objective	506:525	Our second objective	506:525	Our second objective was to determine whether these predictions differ between calves that are fed a high- or low-lactose MR in later life.
28822541	13	84	from	variation	2190:2198	arg1	efficiency					2208:2217	feed efficiency	2203:2217	feed efficiency	2203:2217	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	13	84	from	variation	2190:2198	arg1	life					2228:2231	later life	2222:2231	later life	2222:2231	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	0	85	from	calves	52:57	arg1	%					6:6	Only 7%	0:6	Only 7% of the variation in feed efficiency in veal calves	0:57	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	0	85	from	calves	52:57	arg1	variation					15:23	the variation	11:23	the variation in feed efficiency in veal calves	11:57	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	9	86	from	Observations	1601:1612	arg1	period					1617:1622	period 1	1617:1624	period 1	1617:1624	Observations in period 1 were clustered per category using principal component analysis, and the resulting principal components were used to predict performance in period 2 using multiple regression procedures.
28822541	3	87	from	MR	628:629	arg1	life					640:643	later life	634:643	later life	634:643	Our second objective was to determine whether these predictions differ between calves that are fed a high- or low-lactose MR in later life.
28822541	1	88	from	variation	207:215	arg1	performance					227:237	growth performance	220:237	growth performance	220:237	High interindividual variation in growth performance is commonly observed in veal calf production and appears to depend on milk replacer (MR) composition.
28822541	8	89	theme	Average	1442:1448	arg1	ADG					1462:1464	ADG	1462:1464	ADG	1462:1464	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	8	89	theme	Average	1442:1448	arg1	gain					1456:1459	Average daily gain	1442:1459	Average daily gain (ADG) in period 2	1442:1477	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	8	90	theme	1,292	1520:1524	arg1	g/d					1532:1534	1,292 ± 111 g/d	1520:1534	1,292 ± 111 g/d	1520:1534	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	7	91	theme	healthy	1426:1432	arg1	calves					1434:1439	117 clinically healthy calves	1411:1439	117 clinically healthy calves	1411:1439	Relations between early life characteristics and growth performance in later life were assessed in 117 clinically healthy calves.
28822541	13	92	from	%	2181:2181	arg1	efficiency					2208:2217	feed efficiency	2203:2217	feed efficiency	2203:2217	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	13	92	from	%	2181:2181	arg1	life					2228:2231	later life	2222:2231	later life	2222:2231	This indicates that >90% of the variation in feed efficiency in later life could not be explained by early life characterization of the calves.
28822541	12	93	theme	standardized	2045:2056	arg1	ADG					2058:2060	standardized ADG	2045:2060	standardized ADG in period 2	2045:2072	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	9	94	theme	resulting	1698:1706	arg1	components					1718:1727	the resulting principal components	1694:1727	the resulting principal components	1694:1727	Observations in period 1 were clustered per category using principal component analysis, and the resulting principal components were used to predict performance in period 2 using multiple regression procedures.
28822541	8	95	from	gain	1456:1459	arg1	period					1470:1475	period 2	1470:1477	period 2	1470:1477	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	9	96	used	used	1734:1737	arg2	components					1718:1727	the resulting principal components	1694:1727	the resulting principal components	1694:1727	Observations in period 1 were clustered per category using principal component analysis, and the resulting principal components were used to predict performance in period 2 using multiple regression procedures.
28822541	15	97	dep	life	2563:2566	arg1	i.e.					2569:2572	i.e.	2569:2572	i.e.	2569:2572	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	15	98	from	antibodies	2543:2552	arg1	life					2563:2566	early life	2557:2566	early life (i.e., not exposed to the lactose replacer)	2557:2610	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	11	99	theme	feed	1952:1955	arg1	refusals					1957:1964	solid feed refusals	1946:1964	solid feed refusals	1946:1964	However, this was mainly related to variation in solid feed refusals.
28822541	12	100	theme	feed	2004:2007	arg1	intake					2009:2014	equal solid feed intake	1992:2014	equal solid feed intake	1992:2014	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	16	101	from	calves	2737:2742	arg1	ability					2767:2773	their ability to cope with MR varying in lactose content	2761:2816	their ability to cope with MR varying in lactose content	2761:2816	These measurements are therefore potential tools for screening calves in early life on their ability to cope with MR varying in lactose content.
28822541	16	101	from	calves	2737:2742	arg1	life					2753:2756	early life	2747:2756	early life on their ability to cope with MR varying in lactose content	2747:2816	These measurements are therefore potential tools for screening calves in early life on their ability to cope with MR varying in lactose content.
28822541	15	102	from	concentrations	2482:2495	arg1	life					2563:2566	early life	2557:2566	early life (i.e., not exposed to the lactose replacer)	2557:2610	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	14	103	from	variation	2324:2332	arg1	status					2344:2349	health status	2337:2349	health status	2337:2349	It is speculated that variation in health status explains a substantial portion of variation in feed efficiency in later life.
28822541	12	104	theme	early	2133:2137	arg1	measurements					2144:2155	early life measurements	2133:2155	early life measurements	2133:2155	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	6	105	contain	contained	1135:1143	arg2	source					1178:1183	the only carbohydrate source	1156:1183	the only carbohydrate source	1156:1183	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	105	contain	contained	1135:1143	arg2	lactose					1145:1151	lactose	1145:1151	lactose	1145:1151	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	105	contain	contained	1135:1143	arg1	treatments					1105:1114	2 MR treatments	1100:1114	2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio)	1100:1309	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	6	105	contain	contained	1135:1143	arg1	MR					1127:1128	a control MR	1117:1128	a control MR that contained lactose as the only carbohydrate source	1117:1183	In period 2 (wk 10-26), 130 calves were equally divided over 2 MR treatments: a control MR that contained lactose as the only carbohydrate source and a low-lactose MR in which 51% of the lactose was isocalorically replaced by glucose, fructose, and glycerol (2:1:2 ratio).
28822541	2	106	theme	veal	427:430	arg1	calves					432:437	healthy veal calves	419:437	healthy veal calves	419:437	Our first objective was to examine whether variation in growth performance in healthy veal calves can be predicted from early life characterization of these calves.
28822541	12	107	theme	variation	2032:2040	arg1	%					2023:2023	only 7%	2017:2023	only 7% of the variation in standardized ADG in period 2	2017:2072	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	12	107	theme	variation	2032:2040	arg1	variation					2032:2040	the variation	2028:2040	the variation in standardized ADG in period 2	2028:2072	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	8	108	theme	low-lactose	1567:1577	arg1	g/d					1595:1597	1,267 ± 103 g/d	1583:1597	1,267 ± 103 g/d	1583:1597	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	8	108	theme	low-lactose	1567:1577	arg1	MR					1579:1580	the low-lactose MR	1563:1580	the low-lactose MR (1,267 ± 103 g/d)	1563:1598	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	15	109	theme	plasma	2528:2533	arg1	antibodies					2543:2552	plasma natural antibodies	2528:2552	plasma natural antibodies	2528:2552	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	7	110	theme	growth	1361:1366	arg1	performance					1368:1378	growth performance	1361:1378	growth performance	1361:1378	Relations between early life characteristics and growth performance in later life were assessed in 117 clinically healthy calves.
28822541	2	111	theme	early	461:465	arg1	characterization					472:487	early life characterization	461:487	early life characterization of these calves	461:503	Our first objective was to examine whether variation in growth performance in healthy veal calves can be predicted from early life characterization of these calves.
28822541	2	112	from	performance	404:414	arg1	calves					432:437	healthy veal calves	419:437	healthy veal calves	419:437	Our first objective was to examine whether variation in growth performance in healthy veal calves can be predicted from early life characterization of these calves.
28822541	10	113	from	Variation	1812:1820	arg1	observations					1825:1836	observations	1825:1836	observations in period 1	1825:1848	Variation in observations in period 1 predicted 17% of variation in ADG in period 2.
28822541	14	114	from	efficiency	2403:2412	arg1	life					2423:2426	later life	2417:2426	later life	2417:2426	It is speculated that variation in health status explains a substantial portion of variation in feed efficiency in later life.
28822541	1	115	theme	replacer	314:321	arg1	composition					328:338	milk replacer (MR) composition	309:338	milk replacer (MR) composition	309:338	High interindividual variation in growth performance is commonly observed in veal calf production and appears to depend on milk replacer (MR) composition.
28822541	14	116	theme	later	2417:2421	arg1	life					2423:2426	later life	2417:2426	later life	2417:2426	It is speculated that variation in health status explains a substantial portion of variation in feed efficiency in later life.
28822541	0	117	from	variation	15:23	arg1	efficiency					33:42	feed efficiency	28:42	feed efficiency	28:42	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	0	117	from	variation	15:23	arg1	calves					52:57	veal calves	47:57	veal calves	47:57	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	9	118	theme	multiple	1780:1787	arg1	procedures					1800:1809	multiple regression procedures	1780:1809	multiple regression procedures	1780:1809	Observations in period 1 were clustered per category using principal component analysis, and the resulting principal components were used to predict performance in period 2 using multiple regression procedures.
28822541	10	119	from	observations	1825:1836	arg1	period					1841:1846	period 1	1841:1848	period 1	1841:1848	Variation in observations in period 1 predicted 17% of variation in ADG in period 2.
28822541	1	120	theme	MR	324:325	arg1	composition					328:338	milk replacer (MR) composition	309:338	milk replacer (MR) composition	309:338	High interindividual variation in growth performance is commonly observed in veal calf production and appears to depend on milk replacer (MR) composition.
28822541	8	121	theme	±	1589:1589	arg1	g/d					1595:1597	1,267 ± 103 g/d	1583:1597	1,267 ± 103 g/d	1583:1597	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	8	121	theme	±	1589:1589	arg1	MR					1579:1580	the low-lactose MR	1563:1580	the low-lactose MR (1,267 ± 103 g/d)	1563:1598	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	5	122	theme	postabsorptive	975:988	arg1	metabolism					990:999	postabsorptive metabolism	975:999	postabsorptive metabolism	975:999	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	5	122	theme	postabsorptive	975:988	arg1	categories					932:941	5 categories	930:941	5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology	930:1036	Subsequently, calves were characterized in the following 9 wk (period 1) using targeted challenges related to traits within each of 5 categories: feeding motivation, digestion, postabsorptive metabolism, behavior and stress, and immunology.
28822541	8	123	dep	greater	1492:1498	arg1	g/d					1532:1534	1,292 ± 111 g/d	1520:1534	1,292 ± 111 g/d	1520:1534	Average daily gain (ADG) in period 2 tended to be greater for control calves (1,292 ± 111 g/d) than for calves receiving the low-lactose MR (1,267 ± 103 g/d).
28822541	12	124	from	variation	2032:2040	arg1	ADG					2058:2060	standardized ADG	2045:2060	standardized ADG in period 2	2045:2072	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	15	125	theme	feed	2616:2619	arg1	efficiency					2621:2630	feed efficiency	2616:2630	feed efficiency in later life	2616:2644	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	0	126	from	immunology	137:146	arg1	life					180:183	early life	174:183	early life	174:183	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	4	127	theme	Holstein-Friesian	666:682	arg1	calves					684:689	180 male Holstein-Friesian calves	657:689	180 male Holstein-Friesian calves	657:689	A total of 180 male Holstein-Friesian calves arrived at the facilities at 17 ± 3.4 d of age, and blood samples were collected before the first feeding.
28822541	0	128	theme	variation	15:23	arg1	%					6:6	Only 7%	0:6	Only 7% of the variation in feed efficiency in veal calves	0:57	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	0	128	theme	variation	15:23	arg1	variation					15:23	the variation	11:23	the variation in feed efficiency in veal calves	11:57	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	2	129	theme	first	345:349	arg1	objective					351:359	Our first objective	341:359	Our first objective	341:359	Our first objective was to examine whether variation in growth performance in healthy veal calves can be predicted from early life characterization of these calves.
28822541	0	130	theme	feed	28:31	arg1	efficiency					33:42	feed efficiency	28:42	feed efficiency	28:42	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	1	131	theme	calf	268:271	arg1	production					273:282	veal calf production	263:282	veal calf production	263:282	High interindividual variation in growth performance is commonly observed in veal calf production and appears to depend on milk replacer (MR) composition.
28822541	7	132	theme	life	1336:1339	arg1	characteristics					1341:1355	early life characteristics	1330:1355	early life characteristics	1330:1355	Relations between early life characteristics and growth performance in later life were assessed in 117 clinically healthy calves.
28822541	12	133	theme	equal	1992:1996	arg1	intake					2009:2014	equal solid feed intake	1992:2014	equal solid feed intake	1992:2014	When ADG was adjusted to equal solid feed intake, only 7% of the variation in standardized ADG in period 2, in fact reflecting feed efficiency, could be explained by early life measurements.
28822541	14	134	theme	substantial	2362:2372	arg1	portion					2374:2380	a substantial portion	2360:2380	a substantial portion of variation	2360:2393	It is speculated that variation in health status explains a substantial portion of variation in feed efficiency in later life.
28822541	2	135	theme	growth	397:402	arg1	performance					404:414	growth performance	397:414	growth performance in healthy veal calves	397:437	Our first objective was to examine whether variation in growth performance in healthy veal calves can be predicted from early life characterization of these calves.
28822541	0	136	from	traits	164:169	arg1	life					180:183	early life	174:183	early life	174:183	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	11	137	from	variation	1933:1941	arg1	refusals					1957:1964	solid feed refusals	1946:1964	solid feed refusals	1946:1964	However, this was mainly related to variation in solid feed refusals.
28822541	4	138	theme	age	734:736	arg1	d					729:729	17 ± 3.4 d	720:729	17 ± 3.4 d of age	720:736	A total of 180 male Holstein-Friesian calves arrived at the facilities at 17 ± 3.4 d of age, and blood samples were collected before the first feeding.
28822541	15	139	from	efficiency	2621:2630	arg1	life					2641:2644	later life	2635:2644	later life	2635:2644	Significant relations between fasting plasma glucose concentrations, fecal pH, drinking speed, and plasma natural antibodies in early life (i.e., not exposed to the lactose replacer) and feed efficiency in later life depended on MR composition.
28822541	0	140	from	motivation	102:111	arg1	life					180:183	early life	174:183	early life	174:183	Only 7% of the variation in feed efficiency in veal calves can be predicted from variation in feeding motivation, digestion, metabolism, immunology, and behavioral traits in early life.
28822541	10	141	from	ADG	1880:1882	arg1	period					1887:1892	period 2	1887:1894	period 2	1887:1894	Variation in observations in period 1 predicted 17% of variation in ADG in period 2.
28822541	14	142	theme	health	2337:2342	arg1	status					2344:2349	health status	2337:2349	health status	2337:2349	It is speculated that variation in health status explains a substantial portion of variation in feed efficiency in later life.
28822541	3	143	theme	later	634:638	arg1	life					640:643	later life	634:643	later life	634:643	Our second objective was to determine whether these predictions differ between calves that are fed a high- or low-lactose MR in later life.
28822541	4	144	theme	±	723:723	arg1	d					729:729	17 ± 3.4 d	720:729	17 ± 3.4 d of age	720:736	A total of 180 male Holstein-Friesian calves arrived at the facilities at 17 ± 3.4 d of age, and blood samples were collected before the first feeding.
28822541	1	145	theme	interindividual	191:205	arg1	variation					207:215	High interindividual variation	186:215	High interindividual variation in growth performance	186:237	High interindividual variation in growth performance is commonly observed in veal calf production and appears to depend on milk replacer (MR) composition.
27582072	1	0	dep	mucoadhere	298:307	arg1	curcumin					321:328	curcumin	321:328	curcumin	321:328	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	5	1	from	matrix	1009:1014	arg1	media					1024:1028	acid media	1019:1028	acid media	1019:1028	SEM images of the nanoparticles after exposure to the various media indicate a retained matrix in acid media as opposed to a distorted/fragmented matrix in pectinase-enriched medium.
27582072	3	2	with	nanopolymeric	526:538	arg1	z-average					547:555	a z-average	545:555	a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%	545:654	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	1	3	theme	mucoadhesive	141:152	arg1	formulation					189:199	a mucoadhesive chitosan-pectinate nanoparticulate formulation	139:199	a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract	139:278	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	1	4	theme	upper	251:255	arg1	tract					274:278	the upper gastrointestinal tract	247:278	the upper gastrointestinal tract	247:278	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	5	5	theme	pectinase-enriched	1077:1094	arg1	medium					1096:1101	pectinase-enriched medium	1077:1101	pectinase-enriched medium	1077:1101	SEM images of the nanoparticles after exposure to the various media indicate a retained matrix in acid media as opposed to a distorted/fragmented matrix in pectinase-enriched medium.
27582072	5	6	theme	SEM	921:923	arg1	images					925:930	SEM images	921:930	SEM images of the nanoparticles after exposure to the various media	921:987	SEM images of the nanoparticles after exposure to the various media indicate a retained matrix in acid media as opposed to a distorted/fragmented matrix in pectinase-enriched medium.
27582072	1	7	theme	gastrointestinal	257:272	arg1	tract					274:278	the upper gastrointestinal tract	247:278	the upper gastrointestinal tract	247:278	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	1	8	from	release	313:319	arg1	conditions					339:348	colon conditions	333:348	colon conditions	333:348	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	5	9	dep	matrix	1009:1014	arg1	opposed					1033:1039	opposed	1033:1039	opposed to a distorted/fragmented matrix in pectinase-enriched medium	1033:1101	SEM images of the nanoparticles after exposure to the various media indicate a retained matrix in acid media as opposed to a distorted/fragmented matrix in pectinase-enriched medium.
27582072	1	10	theme	tract	274:278	arg1	milieu					237:242	the milieu	233:242	the milieu of the upper gastrointestinal tract	233:278	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	5	11	theme	distorted/fragmented	1046:1065	arg1	matrix					1067:1072	a distorted/fragmented matrix	1044:1072	a distorted/fragmented matrix in pectinase-enriched medium	1044:1101	SEM images of the nanoparticles after exposure to the various media indicate a retained matrix in acid media as opposed to a distorted/fragmented matrix in pectinase-enriched medium.
27582072	4	12	dep	release	768:774	arg1	%					766:766	%	766:766	%	766:766	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	1	13	theme	chitosan-pectinate	154:171	arg1	formulation					189:199	a mucoadhesive chitosan-pectinate nanoparticulate formulation	139:199	a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract	139:278	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	4	14	from	pH	913:914	arg1	release					864:870	negligible release	853:870	negligible release in acidic and enzyme-restricted media at pH 6.8	853:918	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	1	15	from	mucoadhere	298:307	arg1	conditions					339:348	colon conditions	333:348	colon conditions	333:348	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	5	16	theme	nanoparticles	939:951	arg1	images					925:930	SEM images	921:930	SEM images of the nanoparticles after exposure to the various media	921:987	SEM images of the nanoparticles after exposure to the various media indicate a retained matrix in acid media as opposed to a distorted/fragmented matrix in pectinase-enriched medium.
27582072	1	17	theme	nanoparticulate	173:187	arg1	formulation					189:199	a mucoadhesive chitosan-pectinate nanoparticulate formulation	139:199	a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract	139:278	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	5	18	from	matrix	1067:1072	arg1	medium					1096:1101	pectinase-enriched medium	1077:1101	pectinase-enriched medium	1077:1101	SEM images of the nanoparticles after exposure to the various media indicate a retained matrix in acid media as opposed to a distorted/fragmented matrix in pectinase-enriched medium.
27582072	6	19	theme	delivery	1222:1229	arg1	system					1231:1236	a colon-targeted mucoadhesive curcumin delivery system	1183:1236	a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer	1183:1279	The data strongly indicates that the system has the potential to be applied as a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer.
27582072	1	20	theme	formulation	189:199	arg1	properties					125:134	the properties	121:134	the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract	121:278	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	0	21	theme	Mucoadhesive	0:11	arg1	Chitosan-Pectinate					13:30	Mucoadhesive Chitosan-Pectinate	0:30	Mucoadhesive Chitosan-Pectinate	0:30	Mucoadhesive Chitosan-Pectinate Nanoparticles for the Delivery of Curcumin to the Colon.
27582072	5	22	theme	acid	1019:1022	arg1	media					1024:1028	acid media	1019:1028	acid media	1019:1028	SEM images of the nanoparticles after exposure to the various media indicate a retained matrix in acid media as opposed to a distorted/fragmented matrix in pectinase-enriched medium.
27582072	1	23	theme	able	201:204	arg1	formulation					189:199	a mucoadhesive chitosan-pectinate nanoparticulate formulation	139:199	a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract	139:278	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	2	24	theme	possible	420:427	arg1	management					429:438	the possible management	416:438	the possible management of colorectal cancer	416:459	Using this system, we aimed to deliver curcumin to the colon for the possible management of colorectal cancer.
27582072	4	25	theme	curcumin	779:786	arg1	release					768:774	more than 80% release	754:774	more than 80% release of curcumin	754:786	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	4	26	theme	enzyme-restricted	886:902	arg1	media					904:908	acidic and enzyme-restricted media	875:908	acidic and enzyme-restricted media	875:908	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	1	27	theme	present	96:102	arg1	study					104:108	the present study	92:108	the present study	92:108	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	5	28	theme	various	975:981	arg1	media					983:987	the various media	971:987	the various media	971:987	SEM images of the nanoparticles after exposure to the various media indicate a retained matrix in acid media as opposed to a distorted/fragmented matrix in pectinase-enriched medium.
27582072	3	29	theme	efficiency	638:647	arg1	potential					588:596	zeta potential	583:596	zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%	583:654	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	3	29	theme	efficiency	638:647	arg1	±6.6 nm					570:576	±6.6 nm	570:576	±6.6 nm	570:576	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	3	29	theme	efficiency	638:647	arg1	206.0 nm					560:567	206.0 nm	560:567	206.0 nm (±6.6 nm)	560:577	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	3	30	theme	chitosan-pectinate	497:514	arg1	nanopolymeric					526:538	a chitosan-pectinate composite nanopolymeric	495:538	a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%	495:654	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	3	31	theme	potential	588:596	arg1	z-average					547:555	a z-average	545:555	a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%	545:654	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	4	32	dep	%	766:766	arg1	80					764:765	80	764:765	80	764:765	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	6	33	theme	mucoadhesive	1200:1211	arg1	system					1231:1236	a colon-targeted mucoadhesive curcumin delivery system	1183:1236	a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer	1183:1279	The data strongly indicates that the system has the potential to be applied as a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer.
27582072	6	34	theme	colon-targeted	1185:1198	arg1	system					1231:1236	a colon-targeted mucoadhesive curcumin delivery system	1183:1236	a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer	1183:1279	The data strongly indicates that the system has the potential to be applied as a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer.
27582072	2	35	theme	cancer	454:459	arg1	management					429:438	the possible management	416:438	the possible management of colorectal cancer	416:459	Using this system, we aimed to deliver curcumin to the colon for the possible management of colorectal cancer.
27582072	3	36	theme	encapsulation	624:636	arg1	efficiency					638:647	encapsulation efficiency	624:647	encapsulation efficiency of 64%	624:654	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	4	37	theme	pectinase-enriched	804:821	arg1	pH					831:832	pH 6.4	831:836	pH 6.4	831:836	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	4	37	theme	pectinase-enriched	804:821	arg1	medium					823:828	pectinase-enriched medium	804:828	pectinase-enriched medium (pH 6.4)	804:837	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	6	38	theme	cancer	1274:1279	arg1	treatment					1255:1263	the possible treatment	1242:1263	the possible treatment of colon cancer	1242:1279	The data strongly indicates that the system has the potential to be applied as a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer.
27582072	4	39	theme	alkaline	706:713	arg1	pH					715:716	alkaline pH	706:716	alkaline pH	706:716	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	2	40	theme	colorectal	443:452	arg1	cancer					454:459	colorectal cancer	443:459	colorectal cancer	443:459	Using this system, we aimed to deliver curcumin to the colon for the possible management of colorectal cancer.
27582072	4	41	theme	negligible	853:862	arg1	release					864:870	negligible release	853:870	negligible release in acidic and enzyme-restricted media at pH 6.8	853:918	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	5	42	theme	retained	1000:1007	arg1	matrix					1009:1014	a retained matrix	998:1014	a retained matrix in acid media as opposed to a distorted/fragmented matrix in pectinase-enriched medium	998:1101	SEM images of the nanoparticles after exposure to the various media indicate a retained matrix in acid media as opposed to a distorted/fragmented matrix in pectinase-enriched medium.
27582072	3	43	theme	zeta	583:586	arg1	potential					588:596	zeta potential	583:596	zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%	583:654	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	6	44	theme	possible	1246:1253	arg1	treatment					1255:1263	the possible treatment	1242:1263	the possible treatment of colon cancer	1242:1279	The data strongly indicates that the system has the potential to be applied as a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer.
27582072	4	45	theme	acidic	875:880	arg1	media					904:908	acidic and enzyme-restricted media	875:908	acidic and enzyme-restricted media	875:908	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	3	46	theme	delivery	466:473	arg1	system					475:480	The delivery system	462:480	The delivery system	462:480	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	3	47	theme	206.0 nm	560:567	arg1	z-average					547:555	a z-average	545:555	a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%	545:654	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	6	48	theme	colon	1268:1272	arg1	cancer					1274:1279	colon cancer	1268:1279	colon cancer	1268:1279	The data strongly indicates that the system has the potential to be applied as a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer.
27582072	4	49	from	release	864:870	arg1	media					904:908	acidic and enzyme-restricted media	875:908	acidic and enzyme-restricted media	875:908	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	3	50	theme	%	654:654	arg1	efficiency					638:647	encapsulation efficiency	624:647	encapsulation efficiency of 64%	624:654	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	3	50	theme	%	654:654	arg1	±0.5 mV					611:617	±0.5 mV	611:617	±0.5 mV	611:617	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	3	50	theme	%	654:654	arg1	+32.8 mV					601:608	+32.8 mV	601:608	+32.8 mV (±0.5 mV)	601:618	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	3	51	theme	+32.8 mV	601:608	arg1	potential					588:596	zeta potential	583:596	zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%	583:654	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	3	51	theme	+32.8 mV	601:608	arg1	±6.6 nm					570:576	±6.6 nm	570:576	±6.6 nm	570:576	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	3	51	theme	+32.8 mV	601:608	arg1	206.0 nm					560:567	206.0 nm	560:567	206.0 nm (±6.6 nm)	560:577	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	3	52	theme	composite	516:524	arg1	nanopolymeric					526:538	a chitosan-pectinate composite nanopolymeric	495:538	a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%	495:654	The delivery system comprised of a chitosan-pectinate composite nanopolymeric with a z-average of 206.0 nm (±6.6 nm) and zeta potential of +32.8 mV (±0.5 mV) and encapsulation efficiency of 64%.
27582072	6	53	contain	has	1148:1150	arg1	system					1141:1146	the system	1137:1146	the system	1137:1146	The data strongly indicates that the system has the potential to be applied as a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer.
27582072	6	53	contain	has	1148:1150	arg2	potential					1156:1164	the potential to be applied as a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer	1152:1279	the potential to be applied as a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer	1152:1279	The data strongly indicates that the system has the potential to be applied as a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer.
27582072	0	54	theme	Curcumin	66:73	arg1	Delivery					54:61	the Delivery	50:61	the Delivery of Curcumin to the Colon	50:86	Mucoadhesive Chitosan-Pectinate Nanoparticles for the Delivery of Curcumin to the Colon.
27582072	1	55	theme	colon	333:337	arg1	conditions					339:348	colon conditions	333:348	colon conditions	333:348	In the present study, we report the properties of a mucoadhesive chitosan-pectinate nanoparticulate formulation able to retain its integrity in the milieu of the upper gastrointestinal tract and subsequently, mucoadhere and release curcumin in colon conditions.
27582072	6	56	theme	curcumin	1213:1220	arg1	system					1231:1236	a colon-targeted mucoadhesive curcumin delivery system	1183:1236	a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer	1183:1279	The data strongly indicates that the system has the potential to be applied as a colon-targeted mucoadhesive curcumin delivery system for the possible treatment of colon cancer.
27582072	4	57	theme	nanoparticles	661:673	arg1	higher					696:701	higher	696:701	higher	696:701	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
27582072	4	57	theme	nanoparticles	661:673	arg1	mucoadhesiveness					675:690	The nanoparticles mucoadhesiveness	657:690	The nanoparticles mucoadhesiveness	657:690	The nanoparticles mucoadhesiveness was higher at alkaline pH compared to acidic pH. Furthermore, more than 80% release of curcumin was achieved in pectinase-enriched medium (pH 6.4) as opposed to negligible release in acidic and enzyme-restricted media at pH 6.8.
25791762	8	0	theme	inner	1691:1695	arg1	structure					1705:1713	a more porous inner implant structure	1677:1713	a more porous inner implant structure	1677:1713	This and the higher polymer lipophilicity result in more rapid PLGA precipitation and a more porous inner implant structure.
25791762	3	1	from	systems	472:478	arg1	control					429:435	the control	425:435	the control of drug release in these composite systems	425:478	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	3	1	from	systems	472:478	arg1	processes					381:389	the processes	377:389	the processes involved in implant formation	377:419	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	3	1	from	systems	472:478	arg1	complex					484:490	complex	484:490	complex	484:490	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	8	2	theme	implant	1697:1703	arg1	structure					1705:1713	a more porous inner implant structure	1677:1713	a more porous inner implant structure	1677:1713	This and the higher polymer lipophilicity result in more rapid PLGA precipitation and a more porous inner implant structure.
25791762	6	3	theme	opposite	1152:1159	arg1	impact					1161:1166	This opposite impact	1147:1166	This opposite impact on drug release	1147:1182	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	5	4	theme	chain	1094:1098	arg1	PLGA					1100:1103	longer chain PLGA	1087:1103	longer chain PLGA	1087:1103	Interestingly, HPMC addition to shorter chain PLGA slightly slows down drug release, whereas in the case of longer chain PLGA the release rate substantially increases.
25791762	4	5	theme	characterization	618:633	arg1	techniques					635:644	advanced characterization techniques	609:644	advanced characterization techniques (e.g., electron paramagnetic resonance, EPR)	609:689	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	4	5	theme	characterization	618:633	arg1	resonance					675:683	electron paramagnetic resonance	653:683	electron paramagnetic resonance	653:683	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	3	6	from	release	445:451	arg1	systems					472:478	these composite systems	456:478	these composite systems	456:478	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	2	7	theme	release	252:258	arg1	rate					260:263	a drug release rate	245:263	a drug release rate controlling polymer	245:283	Recently, composite implants, based on a drug release rate controlling polymer and an adhesive polymer, have been proposed for an efficient local drug treatment.
25791762	2	8	theme	adhesive	292:299	arg1	polymer					301:307	an adhesive polymer	289:307	an adhesive polymer	289:307	Recently, composite implants, based on a drug release rate controlling polymer and an adhesive polymer, have been proposed for an efficient local drug treatment.
25791762	4	9	dep	implants	745:752	arg1	in-situ					729:735	the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs	725:976	the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs	725:976	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	10	10	theme	chain	1965:1969	arg1	PLGA					1971:1974	longer chain PLGA	1958:1974	longer chain PLGA	1958:1974	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	1	11	theme	disease	197:203	arg1	cause					144:148	the primary cause	132:148	the primary cause of tooth loss in adults and a very wide-spread disease	132:203	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	1	11	theme	disease	197:203	arg1	Periodontitis					115:127	Periodontitis	115:127	Periodontitis	115:127	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	9	12	theme	drug	1730:1733	arg1	release					1735:1741	drug release	1730:1741	drug release	1730:1741	Consequently, drug release is accelerated.
25791762	2	13	theme	drug	352:355	arg1	treatment					357:365	an efficient local drug treatment	333:365	an efficient local drug treatment	333:365	Recently, composite implants, based on a drug release rate controlling polymer and an adhesive polymer, have been proposed for an efficient local drug treatment.
25791762	7	14	theme	HPMC	1481:1484	arg1	addition					1469:1476	the addition	1465:1476	the addition of HPMC	1465:1484	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	10	15	theme	chain	1825:1829	arg1	PLGA					1831:1834	shorter chain PLGA	1817:1834	shorter chain PLGA	1817:1834	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	8	16	theme	higher	1604:1609	arg1	lipophilicity					1619:1631	the higher polymer lipophilicity	1600:1631	the higher polymer lipophilicity	1600:1631	This and the higher polymer lipophilicity result in more rapid PLGA precipitation and a more porous inner implant structure.
25791762	4	17	theme	drug	827:830	arg1	rate					840:843	drug release rate	827:843	drug release rate controlling polymers	827:864	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	10	18	dep	presence	1861:1868	arg1	the					1857:1859	the	1857:1859	the	1857:1859	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	4	19	theme	methylcellulose	886:900	arg1	types					778:782	(i) different types	764:782	(i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs	764:976	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	6	20	theme	polymer	1261:1267	arg1	weight					1279:1284	the polymer molecular weight	1257:1284	the polymer molecular weight of the PLGA	1257:1296	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	6	20	theme	polymer	1261:1267	arg1	difference					1222:1231	the only difference	1213:1231	the only difference in the formulations	1213:1251	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	5	21	theme	shorter	1011:1017	arg1	PLGA					1025:1028	shorter chain PLGA	1011:1028	shorter chain PLGA	1011:1028	Interestingly, HPMC addition to shorter chain PLGA slightly slows down drug release, whereas in the case of longer chain PLGA the release rate substantially increases.
25791762	4	22	dep	types	778:782	arg1	i					765:765	i	765:765	i	765:765	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	1	23	from	adults	167:172	arg1	cause					144:148	the primary cause	132:148	the primary cause of tooth loss in adults and a very wide-spread disease	132:203	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	1	23	from	adults	167:172	arg1	Periodontitis					115:127	Periodontitis	115:127	Periodontitis	115:127	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	1	24	theme	tooth	153:157	arg1	loss					159:162	tooth loss	153:162	tooth loss in adults	153:172	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	7	25	theme	chain	1453:1457	arg1	PLGA					1459:1462	shorter chain PLGA	1445:1462	shorter chain PLGA	1445:1462	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	4	26	theme	paramagnetic	662:673	arg1	techniques					635:644	advanced characterization techniques	609:644	advanced characterization techniques (e.g., electron paramagnetic resonance, EPR)	609:689	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	4	26	theme	paramagnetic	662:673	arg1	resonance					675:683	electron paramagnetic resonance	653:683	electron paramagnetic resonance	653:683	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	7	27	theme	penetration	1540:1550	arg1	facilitation					1518:1529	a much more pronounced facilitation	1495:1529	a much more pronounced facilitation of water penetration into the system (as evidenced by EPR)	1495:1588	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	3	28	from	processes	381:389	arg1	systems					472:478	these composite systems	456:478	these composite systems	456:478	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	1	29	from	loss	159:162	arg1	adults					167:172	adults	167:172	adults	167:172	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	0	30	theme	system	95:100	arg1	performance					102:112	system performance	95:112	system performance	95:112	In-situ forming composite implants for periodontitis treatment: How the formulation determines system performance.
25791762	4	31	dep	doxycycline	940:950	arg1	iii					935:937	iii	935:937	iii	935:937	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	4	32	theme	forming	737:743	arg1	implants					745:752	forming implants	737:752	the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs	725:976	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	0	33	theme	periodontitis	39:51	arg1	treatment					53:61	periodontitis treatment	39:61	periodontitis treatment	39:61	In-situ forming composite implants for periodontitis treatment: How the formulation determines system performance.
25791762	7	34	theme	water	1534:1538	arg1	penetration					1540:1550	water penetration	1534:1550	water penetration into the system (as evidenced by EPR)	1534:1588	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	8	35	theme	porous	1684:1689	arg1	structure					1705:1713	a more porous inner implant structure	1677:1713	a more porous inner implant structure	1677:1713	This and the higher polymer lipophilicity result in more rapid PLGA precipitation and a more porous inner implant structure.
25791762	0	36	theme	composite	16:24	arg1	implants					26:33	composite implants	16:33	composite implants for periodontitis treatment	16:61	In-situ forming composite implants for periodontitis treatment: How the formulation determines system performance.
25791762	7	37	theme	chain	1409:1413	arg1	PLGA					1415:1418	longer chain PLGA	1402:1418	longer chain PLGA	1402:1418	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	4	38	dep	resonance	675:683	arg1	e.g.					647:650	e.g.	647:650	e.g.	647:650	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	4	38	dep	resonance	675:683	arg1	EPR					686:688	EPR	686:688	EPR	686:688	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	4	39	theme	different	768:776	arg1	types					778:782	(i) different types	764:782	(i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs	764:976	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	3	40	from	control	429:435	arg1	systems					472:478	these composite systems	456:478	these composite systems	456:478	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	5	41	theme	drug	1050:1053	arg1	release					1055:1061	drug release	1050:1061	drug release	1050:1061	Interestingly, HPMC addition to shorter chain PLGA slightly slows down drug release, whereas in the case of longer chain PLGA the release rate substantially increases.
25791762	10	42	from	presence	1861:1868	arg1	similar					1846:1852	similar	1846:1852	similar	1846:1852	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	10	43	theme	resulting	1898:1906	arg1	porous					1931:1936	porous	1931:1936	porous	1931:1936	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	10	43	theme	resulting	1898:1906	arg1	implants					1908:1915	the resulting implants	1894:1915	the resulting implants	1894:1915	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	3	44	theme	release	445:451	arg1	control					429:435	the control	425:435	the control of drug release in these composite systems	425:478	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	3	44	theme	release	445:451	arg1	processes					381:389	the processes	377:389	the processes involved in implant formation	377:419	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	3	44	theme	release	445:451	arg1	complex					484:490	complex	484:490	complex	484:490	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	6	45	from	impact	1161:1166	arg1	release					1176:1182	drug release	1171:1182	drug release	1171:1182	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	10	46	from	absence	1874:1880	arg1	similar					1846:1852	similar	1846:1852	similar	1846:1852	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	2	47	theme	local	346:350	arg1	treatment					357:365	an efficient local drug treatment	333:365	an efficient local drug treatment	333:365	Recently, composite implants, based on a drug release rate controlling polymer and an adhesive polymer, have been proposed for an efficient local drug treatment.
25791762	5	48	theme	release	1109:1115	arg1	rate					1117:1120	the release rate	1105:1120	the release rate	1105:1120	Interestingly, HPMC addition to shorter chain PLGA slightly slows down drug release, whereas in the case of longer chain PLGA the release rate substantially increases.
25791762	4	49	theme	doxycycline	940:950	arg1	types					778:782	(i) different types	764:782	(i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs	764:976	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	6	50	theme	drug	1171:1174	arg1	release					1176:1182	drug release	1171:1182	drug release	1171:1182	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	7	51	theme	underlying	1343:1352	arg1	follows					1387:1393	follows	1387:1393	follows	1387:1393	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	7	51	theme	underlying	1343:1352	arg1	mechanisms					1354:1363	the underlying mechanisms	1339:1363	the underlying mechanisms	1339:1363	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	4	52	theme	lactic-co-glycolic	792:809	arg1	poly					787:790	poly	787:790	poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers	787:864	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	4	52	theme	lactic-co-glycolic	792:809	arg1	acid					811:814	lactic-co-glycolic acid	792:814	lactic-co-glycolic acid	792:814	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	4	53	theme	metronidazole	955:967	arg1	types					778:782	(i) different types	764:782	(i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs	764:976	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	5	54	theme	longer	1087:1092	arg1	PLGA					1100:1103	longer chain PLGA	1087:1103	longer chain PLGA	1087:1103	Interestingly, HPMC addition to shorter chain PLGA slightly slows down drug release, whereas in the case of longer chain PLGA the release rate substantially increases.
25791762	10	55	theme	water	1772:1776	arg1	penetration					1778:1788	water penetration	1772:1788	water penetration into formulations based on shorter chain PLGA	1772:1834	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	7	56	theme	physico-chemical	1312:1327	arg1	analyses					1329:1336	the physico-chemical analyses	1308:1336	the physico-chemical analyses	1308:1336	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	4	57	theme	advanced	609:616	arg1	techniques					635:644	advanced characterization techniques	609:644	advanced characterization techniques (e.g., electron paramagnetic resonance, EPR)	609:689	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	4	57	theme	advanced	609:616	arg1	resonance					675:683	electron paramagnetic resonance	653:683	electron paramagnetic resonance	653:683	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	4	58	theme	poly	787:790	arg1	types					778:782	(i) different types	764:782	(i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs	764:976	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	6	59	theme	only	1217:1220	arg1	difference					1222:1231	the only difference	1213:1231	the only difference in the formulations	1213:1251	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	6	59	theme	only	1217:1220	arg1	weight					1279:1284	the polymer molecular weight	1257:1284	the polymer molecular weight of the PLGA	1257:1296	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	8	60	theme	rapid	1648:1652	arg1	precipitation					1659:1671	more rapid PLGA precipitation	1643:1671	more rapid PLGA precipitation	1643:1671	This and the higher polymer lipophilicity result in more rapid PLGA precipitation and a more porous inner implant structure.
25791762	1	61	theme	wide-spread	185:195	arg1	disease					197:203	a very wide-spread disease	178:203	a very wide-spread disease	178:203	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	4	62	theme	release	832:838	arg1	rate					840:843	drug release rate	827:843	drug release rate controlling polymers	827:864	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	10	63	theme	longer	1958:1963	arg1	PLGA					1971:1974	longer chain PLGA	1958:1974	longer chain PLGA	1958:1974	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	10	64	from	contrast	1762:1769	arg1	similar					1846:1852	similar	1846:1852	similar	1846:1852	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	2	65	theme	composite	216:224	arg1	implants					226:233	composite implants	216:233	composite implants	216:233	Recently, composite implants, based on a drug release rate controlling polymer and an adhesive polymer, have been proposed for an efficient local drug treatment.
25791762	10	66	theme	shorter	1817:1823	arg1	PLGA					1831:1834	shorter chain PLGA	1817:1834	shorter chain PLGA	1817:1834	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	4	67	dep	methylcellulose	886:900	arg1	ii					868:869	ii	868:869	ii	868:869	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	2	68	theme	drug	247:250	arg1	rate					260:263	a drug release rate	245:263	a drug release rate controlling polymer	245:283	Recently, composite implants, based on a drug release rate controlling polymer and an adhesive polymer, have been proposed for an efficient local drug treatment.
25791762	1	69	from	disease	197:203	arg1	adults					167:172	adults	167:172	adults	167:172	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	1	70	theme	primary	136:142	arg1	cause					144:148	the primary cause	132:148	the primary cause of tooth loss in adults and a very wide-spread disease	132:203	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	1	70	theme	primary	136:142	arg1	Periodontitis					115:127	Periodontitis	115:127	Periodontitis	115:127	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	6	71	theme	molecular	1269:1277	arg1	weight					1279:1284	the polymer molecular weight	1257:1284	the polymer molecular weight of the PLGA	1257:1296	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	6	71	theme	molecular	1269:1277	arg1	difference					1222:1231	the only difference	1213:1231	the only difference in the formulations	1213:1251	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	8	72	theme	polymer	1611:1617	arg1	lipophilicity					1619:1631	the higher polymer lipophilicity	1600:1631	the higher polymer lipophilicity	1600:1631	This and the higher polymer lipophilicity result in more rapid PLGA precipitation and a more porous inner implant structure.
25791762	7	73	theme	shorter	1445:1451	arg1	PLGA					1459:1462	shorter chain PLGA	1445:1462	shorter chain PLGA	1445:1462	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	4	74	theme	hydroxypropyl	872:884	arg1	methylcellulose					886:900	(ii) hydroxypropyl methylcellulose	867:900	(ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer	867:927	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	4	74	theme	hydroxypropyl	872:884	arg1	HPMC					903:906	HPMC	903:906	HPMC	903:906	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	5	75	theme	PLGA	1100:1103	arg1	case					1079:1082	the case	1075:1082	the case of longer chain PLGA	1075:1103	Interestingly, HPMC addition to shorter chain PLGA slightly slows down drug release, whereas in the case of longer chain PLGA the release rate substantially increases.
25791762	5	76	theme	chain	1019:1023	arg1	PLGA					1025:1028	shorter chain PLGA	1011:1028	shorter chain PLGA	1011:1028	Interestingly, HPMC addition to shorter chain PLGA slightly slows down drug release, whereas in the case of longer chain PLGA the release rate substantially increases.
25791762	1	77	theme	loss	159:162	arg1	cause					144:148	the primary cause	132:148	the primary cause of tooth loss in adults and a very wide-spread disease	132:203	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	1	77	theme	loss	159:162	arg1	Periodontitis					115:127	Periodontitis	115:127	Periodontitis	115:127	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	4	78	theme	electron	653:660	arg1	techniques					635:644	advanced characterization techniques	609:644	advanced characterization techniques (e.g., electron paramagnetic resonance, EPR)	609:689	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	4	78	theme	electron	653:660	arg1	resonance					675:683	electron paramagnetic resonance	653:683	electron paramagnetic resonance	653:683	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	5	79	theme	HPMC	994:997	arg1	addition					999:1006	HPMC addition	994:1006	HPMC addition to shorter chain PLGA	994:1028	Interestingly, HPMC addition to shorter chain PLGA slightly slows down drug release, whereas in the case of longer chain PLGA the release rate substantially increases.
25791762	8	80	theme	PLGA	1654:1657	arg1	precipitation					1659:1671	more rapid PLGA precipitation	1643:1671	more rapid PLGA precipitation	1643:1671	This and the higher polymer lipophilicity result in more rapid PLGA precipitation and a more porous inner implant structure.
25791762	7	81	dep	system	1561:1566	arg1	evidenced					1572:1580	evidenced	1572:1580	evidenced by EPR	1572:1587	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	1	82	from	cause	144:148	arg1	adults					167:172	adults	167:172	adults	167:172	Periodontitis is the primary cause of tooth loss in adults and a very wide-spread disease.
25791762	6	83	from	difference	1222:1231	arg1	formulations					1240:1251	the formulations	1236:1251	the formulations	1236:1251	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	3	84	theme	implant	403:409	arg1	formation					411:419	implant formation	403:419	implant formation	403:419	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	10	85	from	similar	1846:1852	arg1	absence					1874:1880	absence	1874:1880	absence	1874:1880	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	10	85	from	similar	1846:1852	arg1	presence					1861:1868	presence	1861:1868	presence	1861:1868	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	10	85	from	similar	1846:1852	arg1	contrast					1762:1769	contrast	1762:1769	contrast	1762:1769	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	3	86	theme	drug	440:443	arg1	release					445:451	drug release	440:451	drug release in these composite systems	440:478	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	6	87	theme	PLGA	1293:1296	arg1	weight					1279:1284	the polymer molecular weight	1257:1284	the polymer molecular weight of the PLGA	1257:1296	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	6	87	theme	PLGA	1293:1296	arg1	difference					1222:1231	the only difference	1213:1231	the only difference in the formulations	1213:1251	This opposite impact on drug release was rather surprising, since the only difference in the formulations was the polymer molecular weight of the PLGA.
25791762	7	88	theme	pronounced	1507:1516	arg1	facilitation					1518:1529	a much more pronounced facilitation	1495:1529	a much more pronounced facilitation of water penetration into the system (as evidenced by EPR)	1495:1588	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	7	89	theme	longer	1402:1407	arg1	PLGA					1415:1418	longer chain PLGA	1402:1418	longer chain PLGA	1402:1418	Based on the physico-chemical analyses, the underlying mechanisms could be explained as follows: since longer chain PLGA is more hydrophobic than shorter chain PLGA, the addition of HPMC leads to a much more pronounced facilitation of water penetration into the system (as evidenced by EPR).
25791762	4	90	theme	adhesive	912:919	arg1	polymer					921:927	adhesive polymer	912:927	adhesive polymer	912:927	In this study, advanced characterization techniques (e.g., electron paramagnetic resonance, EPR) were applied to better understand the in-situ forming implants based on: (i) different types of poly(lactic-co-glycolic acid) (PLGA) as drug release rate controlling polymers; (ii) hydroxypropyl methylcellulose (HPMC) as adhesive polymer; and (iii) doxycycline or metronidazole as drugs.
25791762	3	91	theme	composite	462:470	arg1	systems					472:478	these composite systems	456:478	these composite systems	456:478	However, the processes involved in implant formation and the control of drug release in these composite systems are complex and the relationships between the systems' composition and the implants' performance are yet unclear.
25791762	10	92	theme	HPMC	1885:1888	arg1	absence					1874:1880	absence	1874:1880	absence	1874:1880	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	10	92	theme	HPMC	1885:1888	arg1	presence					1861:1868	presence	1861:1868	presence	1861:1868	In contrast, water penetration into formulations based on shorter chain PLGA is rather similar in the presence and absence of HPMC and the resulting implants are much less porous than those based on longer chain PLGA.
25791762	2	93	theme	efficient	336:344	arg1	treatment					357:365	an efficient local drug treatment	333:365	an efficient local drug treatment	333:365	Recently, composite implants, based on a drug release rate controlling polymer and an adhesive polymer, have been proposed for an efficient local drug treatment.
27048796	7	0	theme	capable	1312:1318	arg1	scaffoldin					1301:1310	a hexavalent scaffoldin	1288:1310	a hexavalent scaffoldin capable of connecting to the adaptor scaffoldin by the incorporation of an appropriate type II cohesin	1288:1413	In parallel, we designed a hexavalent scaffoldin capable of connecting to the adaptor scaffoldin by the incorporation of an appropriate type II cohesin.
27048796	3	1	theme	scaffoldin	536:545	arg1	polypeptide					547:557	the scaffoldin polypeptide	532:557	the scaffoldin polypeptide	532:557	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	6	2	contain	possesses	1057:1065	arg2	module					1213:1218	a carbohydrate-binding module	1190:1218	a carbohydrate-binding module for targeting to the cellulosic substrate	1190:1260	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	6	2	contain	possesses	1057:1065	arg2	dockerin					1130:1137	a single type II dockerin	1113:1137	a single type II dockerin for interaction with an additional scaffoldin	1113:1183	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	6	2	contain	possesses	1057:1065	arg1	form					1025:1028	an efficient form	1012:1028	an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate	1012:1260	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	6	2	contain	possesses	1057:1065	arg2	cohesins					1080:1087	three type I cohesins	1067:1087	three type I cohesins for enzyme integration	1067:1110	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	12	3	theme	microbial	2141:2149	arg1	inefficient					2184:2194	inefficient	2184:2194	inefficient	2184:2194	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	12	3	theme	microbial	2141:2149	arg1	process					2163:2169	The natural microbial degradation process	2129:2169	The natural microbial degradation process	2129:2169	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	6	4	theme	cellulosic	1241:1250	arg1	substrate					1252:1260	the cellulosic substrate	1237:1260	the cellulosic substrate	1237:1260	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	4	5	theme	designer	726:733	arg1	cellulosomes					735:746	designer cellulosomes	726:746	designer cellulosomes	726:746	Nevertheless, increasing the number of enzymes integrated into designer cellulosomes is critical, in order to further enhance degradation of plant cell wall material.
27048796	14	6	theme	major	2537:2541	arg1	impacts					2543:2549	major impacts	2537:2549	major impacts	2537:2549	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	3	7	theme	limited	564:570	arg1	numbers					572:578	limited numbers	564:578	limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes	564:660	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	1	8	theme	controlled	189:198	arg1	incorporation					200:212	the controlled incorporation	185:212	the controlled incorporation of recombinant dockerin-containing enzymes	185:255	UNLABELLED Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes.
27048796	14	9	theme	biotechnology	2619:2631	arg1	fields					2558:2563	the fields	2554:2563	the fields of environmental pollution, bioenergy production, and biotechnology in general	2554:2642	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	14	10	contain	has	2533:2535	arg1	development					2439:2449	The development	2435:2449	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation	2435:2526	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	14	10	contain	has	2533:2535	arg2	impacts					2543:2549	major impacts	2537:2549	major impacts	2537:2549	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	3	11	theme	hallmark	628:635	arg1	numbers					572:578	limited numbers	564:578	limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes	564:660	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	3	11	theme	hallmark	628:635	arg1	levels					498:503	resultant low expression levels	473:503	resultant low expression levels	473:503	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	3	11	theme	hallmark	628:635	arg1	cleavage					519:526	cleavage	519:526	cleavage	519:526	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	3	11	theme	hallmark	628:635	arg1	instability					506:516	instability	506:516	instability (cleavage) of the scaffoldin polypeptide	506:557	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	6	12	theme	scaffoldin	1041:1050	arg1	form					1025:1028	an efficient form	1012:1028	an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate	1012:1260	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	15	13	theme	enzymes	2885:2891	arg1	number					2875:2880	the number	2871:2880	the number of enzymes included in a single multienzyme complex	2871:2932	The findings reported in this article comprise a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means, thus enabling the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex.
27048796	3	14	theme	cohesin-dockerin	593:608	arg1	hallmark					628:635	available cohesin-dockerin specificities-the hallmark	583:635	available cohesin-dockerin specificities-the hallmark of designer cellulosomes	583:660	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	15	15	theme	biology	2784:2790	arg1	means					2792:2796	synthetic biology means	2774:2796	synthetic biology means	2774:2796	The findings reported in this article comprise a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means, thus enabling the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex.
27048796	1	16	theme	dockerin-containing	229:247	arg1	enzymes					249:255	recombinant dockerin-containing enzymes	217:255	recombinant dockerin-containing enzymes	217:255	UNLABELLED Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes.
27048796	8	17	theme	potent	1544:1549	arg1	cocktail					1561:1568	a potent enzymatic cocktail	1542:1568	a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates	1542:1625	The resultant extended designer cellulosome comprised 8 recombinant enzymes-4 xylanases and 4 cellulases-thereby representing a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates.
27048796	7	18	theme	type	1399:1402	arg1	cohesin					1407:1413	an appropriate type II cohesin	1384:1413	an appropriate type II cohesin	1384:1413	In parallel, we designed a hexavalent scaffoldin capable of connecting to the adaptor scaffoldin by the incorporation of an appropriate type II cohesin.
27048796	12	19	theme	plant-derived	2256:2268	arg1	biomass					2270:2276	plant-derived biomass	2256:2276	plant-derived biomass	2256:2276	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	2	20	theme	designer	270:277	arg1	scaffoldin					322:331	a chimeric scaffoldin	311:331	a chimeric scaffoldin that contains 6 cohesins	311:356	The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
27048796	2	20	theme	designer	270:277	arg1	cellulosome					279:289	The largest designer cellulosome	258:289	The largest designer cellulosome reported to date	258:306	The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
27048796	6	21	theme	efficient	1015:1023	arg1	form					1025:1028	an efficient form	1012:1028	an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate	1012:1260	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	13	22	theme	latter	2381:2386	arg1	accumulation					2361:2372	The accumulation	2357:2372	The accumulation of the latter	2357:2386	The accumulation of the latter is considered a major environmental pollutant.
27048796	12	23	theme	cost-effective	2210:2223	arg1	processes					2225:2233	cost-effective processes	2210:2233	cost-effective processes	2210:2233	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	3	24	theme	designer	640:647	arg1	cellulosomes					649:660	designer cellulosomes	640:660	designer cellulosomes	640:660	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	14	25	theme	cell	2506:2509	arg1	degradation					2516:2526	enhanced plant cell wall degradation	2491:2526	enhanced plant cell wall degradation	2491:2526	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	6	26	theme	enzyme	1093:1098	arg1	integration					1100:1110	enzyme integration	1093:1110	enzyme integration	1093:1110	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	8	27	theme	natural	1592:1598	arg1	substrates					1616:1625	natural lignocellulosic substrates	1592:1625	natural lignocellulosic substrates	1592:1625	The resultant extended designer cellulosome comprised 8 recombinant enzymes-4 xylanases and 4 cellulases-thereby representing a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates.
27048796	14	28	theme	enhanced	2491:2498	arg1	degradation					2516:2526	enhanced plant cell wall degradation	2491:2526	enhanced plant cell wall degradation	2491:2526	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	0	29	theme	Designer	55:62	arg1	Cellulosomes					64:75	Extended Designer Cellulosomes	46:75	Extended Designer Cellulosomes	46:75	Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature.
27048796	10	30	theme	native	1946:1951	arg1	cellulosomes					1953:1964	native cellulosomes	1946:1964	native cellulosomes	1946:1964	After 72 h of incubation, the performance of the extended designer cellulosome was determined to be approximately 70% compared to that of native cellulosomes.
27048796	1	31	theme	cohesin-bearing	153:167	arg1	scaffoldins					169:179	chimeric cohesin-bearing scaffoldins	144:179	chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes	144:255	UNLABELLED Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes.
27048796	8	32	theme	substrates	1616:1625	arg1	solubilization					1574:1587	solubilization	1574:1587	solubilization of natural lignocellulosic substrates	1574:1625	The resultant extended designer cellulosome comprised 8 recombinant enzymes-4 xylanases and 4 cellulases-thereby representing a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates.
27048796	14	33	theme	nanodevices	2475:2485	arg1	development					2439:2449	The development	2435:2449	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation	2435:2526	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	9	34	theme	scaffoldin	1660:1669	arg1	contribution					1632:1643	The contribution	1628:1643	The contribution of the adaptor scaffoldin	1628:1669	The contribution of the adaptor scaffoldin clearly demonstrated that proximity between the two scaffoldins and their composite set of enzymes is crucial for optimized degradation.
27048796	6	35	theme	type	1073:1076	arg1	cohesins					1080:1087	three type I cohesins	1067:1087	three type I cohesins for enzyme integration	1067:1110	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	14	36	theme	designer	2454:2461	arg1	nanodevices					2475:2485	designer cellulosome nanodevices	2454:2485	designer cellulosome nanodevices for enhanced plant cell wall degradation	2454:2526	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	15	37	theme	true	2694:2697	arg1	breakthrough					2699:2710	a true breakthrough	2692:2710	a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means	2692:2796	The findings reported in this article comprise a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means, thus enabling the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex.
27048796	3	38	theme	technical	389:397	arg1	limit					399:403	a technical limit	387:403	a technical limit of sorts	387:412	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	11	39	theme	biofuels	2071:2078	arg1	production					2057:2066	the production	2053:2066	the production of biofuels such as ethanol	2053:2094	IMPORTANCE Plant cell wall residues represent a major source of renewable biomass for the production of biofuels such as ethanol via breakdown to soluble sugars.
27048796	8	40	theme	extended	1430:1437	arg1	cellulosome					1448:1458	The resultant extended designer cellulosome	1416:1458	The resultant extended designer cellulosome	1416:1458	The resultant extended designer cellulosome comprised 8 recombinant enzymes-4 xylanases and 4 cellulases-thereby representing a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates.
27048796	3	41	theme	resultant	473:481	arg1	levels					498:503	resultant low expression levels	473:503	resultant low expression levels	473:503	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	15	42	theme	complexes	2842:2850	arg1	assembly					2817:2824	the assembly	2813:2824	the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex	2813:2932	The findings reported in this article comprise a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means, thus enabling the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex.
27048796	7	43	theme	cohesin	1407:1413	arg1	incorporation					1367:1379	the incorporation	1363:1379	the incorporation of an appropriate type II cohesin	1363:1413	In parallel, we designed a hexavalent scaffoldin capable of connecting to the adaptor scaffoldin by the incorporation of an appropriate type II cohesin.
27048796	4	44	theme	plant	804:808	arg1	material					820:827	plant cell wall material	804:827	plant cell wall material	804:827	Nevertheless, increasing the number of enzymes integrated into designer cellulosomes is critical, in order to further enhance degradation of plant cell wall material.
27048796	0	45	theme	Adaptor	0:6	arg1	Scaffoldins					8:18	Adaptor Scaffoldins	0:18	Adaptor Scaffoldins	0:18	Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature.
27048796	6	46	theme	II	1127:1128	arg1	dockerin					1130:1137	a single type II dockerin	1113:1137	a single type II dockerin for interaction with an additional scaffoldin	1113:1183	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	4	47	theme	wall	815:818	arg1	material					820:827	plant cell wall material	804:827	plant cell wall material	804:827	Nevertheless, increasing the number of enzymes integrated into designer cellulosomes is critical, in order to further enhance degradation of plant cell wall material.
27048796	12	48	theme	human-generated	2322:2336	arg1	wastes					2349:2354	human-generated cellulosic wastes	2322:2354	human-generated cellulosic wastes	2322:2354	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	15	49	theme	multienzyme	2914:2924	arg1	complex					2926:2932	a single multienzyme complex	2905:2932	a single multienzyme complex	2905:2932	The findings reported in this article comprise a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means, thus enabling the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex.
27048796	14	50	from	fields	2558:2563	arg1	general					2636:2642	general	2636:2642	general	2636:2642	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	5	51	attach	attach	940:945	arg2	type					875:878	an intermediate type	859:878	an intermediate type of scaffoldin that can both incorporate various enzymes and attach to an additional scaffoldin	859:973	Adaptor scaffoldins comprise an intermediate type of scaffoldin that can both incorporate various enzymes and attach to an additional scaffoldin.
27048796	5	51	attach	attach	940:945	arg1	scaffoldin					964:973	an additional scaffoldin	950:973	an additional scaffoldin	950:973	Adaptor scaffoldins comprise an intermediate type of scaffoldin that can both incorporate various enzymes and attach to an additional scaffoldin.
27048796	1	52	theme	UNLABELLED	100:109	arg1	cellulosomes					120:131	UNLABELLED Designer cellulosomes	100:131	UNLABELLED Designer cellulosomes	100:131	UNLABELLED Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes.
27048796	6	53	theme	single	1115:1120	arg1	dockerin					1130:1137	a single type II dockerin	1113:1137	a single type II dockerin for interaction with an additional scaffoldin	1113:1183	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	8	54	theme	enzymes-4	1484:1492	arg1	xylanases					1494:1502	8 recombinant enzymes-4 xylanases	1470:1502	8 recombinant enzymes-4 xylanases	1470:1502	The resultant extended designer cellulosome comprised 8 recombinant enzymes-4 xylanases and 4 cellulases-thereby representing a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates.
27048796	3	55	theme	expression	487:496	arg1	levels					498:503	resultant low expression levels	473:503	resultant low expression levels	473:503	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	0	56	theme	Original	24:31	arg1	Strategy					33:40	An Original Strategy	21:40	An Original Strategy for Extended Designer Cellulosomes	21:75	Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature.
27048796	2	57	contain	contains	338:345	arg1	scaffoldin					322:331	a chimeric scaffoldin	311:331	a chimeric scaffoldin that contains 6 cohesins	311:356	The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
27048796	2	57	contain	contains	338:345	arg2	cohesins					349:356	6 cohesins	347:356	6 cohesins	347:356	The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
27048796	2	57	contain	contains	338:345	arg1	cellulosome					279:289	The largest designer cellulosome	258:289	The largest designer cellulosome reported to date	258:306	The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
27048796	6	58	with	interaction	1143:1153	arg1	scaffoldin					1174:1183	an additional scaffoldin	1160:1183	an additional scaffoldin	1160:1183	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	11	59	theme	wall	1989:1992	arg1	residues					1994:2001	IMPORTANCE Plant cell wall residues	1967:2001	IMPORTANCE Plant cell wall residues	1967:2001	IMPORTANCE Plant cell wall residues represent a major source of renewable biomass for the production of biofuels such as ethanol via breakdown to soluble sugars.
27048796	14	60	theme	bioenergy	2593:2601	arg1	production					2603:2612	bioenergy production	2593:2612	bioenergy production	2593:2612	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	11	61	theme	Plant	1978:1982	arg1	residues					1994:2001	IMPORTANCE Plant cell wall residues	1967:2001	IMPORTANCE Plant cell wall residues	1967:2001	IMPORTANCE Plant cell wall residues represent a major source of renewable biomass for the production of biofuels such as ethanol via breakdown to soluble sugars.
27048796	15	62	theme	synthetic	2774:2782	arg1	means					2792:2796	synthetic biology means	2774:2796	synthetic biology means	2774:2796	The findings reported in this article comprise a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means, thus enabling the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex.
27048796	10	63	theme	cellulosome	1875:1885	arg1	performance					1838:1848	the performance	1834:1848	the performance of the extended designer cellulosome	1834:1885	After 72 h of incubation, the performance of the extended designer cellulosome was determined to be approximately 70% compared to that of native cellulosomes.
27048796	10	63	theme	cellulosome	1875:1885	arg1	%					1924:1924	approximately 70%	1908:1924	approximately 70%	1908:1924	After 72 h of incubation, the performance of the extended designer cellulosome was determined to be approximately 70% compared to that of native cellulosomes.
27048796	9	64	theme	enzymes	1762:1768	arg1	enzymes					1762:1768	enzymes	1762:1768	enzymes	1762:1768	The contribution of the adaptor scaffoldin clearly demonstrated that proximity between the two scaffoldins and their composite set of enzymes is crucial for optimized degradation.
27048796	9	64	theme	enzymes	1762:1768	arg1	set					1755:1757	their composite set	1739:1757	their composite set of enzymes	1739:1768	The contribution of the adaptor scaffoldin clearly demonstrated that proximity between the two scaffoldins and their composite set of enzymes is crucial for optimized degradation.
27048796	9	64	theme	enzymes	1762:1768	arg1	scaffoldins					1723:1733	the two scaffoldins	1715:1733	the two scaffoldins	1715:1733	The contribution of the adaptor scaffoldin clearly demonstrated that proximity between the two scaffoldins and their composite set of enzymes is crucial for optimized degradation.
27048796	7	65	theme	adaptor	1341:1347	arg1	scaffoldin					1349:1358	the adaptor scaffoldin	1337:1358	the adaptor scaffoldin	1337:1358	In parallel, we designed a hexavalent scaffoldin capable of connecting to the adaptor scaffoldin by the incorporation of an appropriate type II cohesin.
27048796	14	66	theme	pollution	2582:2590	arg1	fields					2558:2563	the fields	2554:2563	the fields of environmental pollution, bioenergy production, and biotechnology in general	2554:2642	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	3	67	theme	polypeptide	547:557	arg1	numbers					572:578	limited numbers	564:578	limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes	564:660	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	3	67	theme	polypeptide	547:557	arg1	levels					498:503	resultant low expression levels	473:503	resultant low expression levels	473:503	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	3	67	theme	polypeptide	547:557	arg1	cleavage					519:526	cleavage	519:526	cleavage	519:526	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	3	67	theme	polypeptide	547:557	arg1	instability					506:516	instability	506:516	instability (cleavage) of the scaffoldin polypeptide	506:557	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	0	68	theme	Extended	46:53	arg1	Cellulosomes					64:75	Extended Designer Cellulosomes	46:75	Extended Designer Cellulosomes	46:75	Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature.
27048796	6	69	theme	additional	1163:1172	arg1	scaffoldin					1174:1183	an additional scaffoldin	1160:1183	an additional scaffoldin	1160:1183	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	13	70	theme	major	2404:2408	arg1	pollutant					2424:2432	a major environmental pollutant	2402:2432	a major environmental pollutant	2402:2432	The accumulation of the latter is considered a major environmental pollutant.
27048796	15	71	theme	extended	2739:2746	arg1	cellulosomes					2757:2768	extended designer cellulosomes	2739:2768	extended designer cellulosomes	2739:2768	The findings reported in this article comprise a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means, thus enabling the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex.
27048796	9	72	theme	optimized	1785:1793	arg1	degradation					1795:1805	optimized degradation	1785:1805	optimized degradation	1785:1805	The contribution of the adaptor scaffoldin clearly demonstrated that proximity between the two scaffoldins and their composite set of enzymes is crucial for optimized degradation.
27048796	12	73	dep	conversion	2242:2251	arg1	either					2291:2296	either	2291:2296	either	2291:2296	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	12	74	link	plant-derived	2256:2268	arg1	biomass					2270:2276	plant-derived biomass	2256:2276	plant-derived biomass	2256:2276	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	13	75	theme	environmental	2410:2422	arg1	pollutant					2424:2432	a major environmental pollutant	2402:2432	a major environmental pollutant	2402:2432	The accumulation of the latter is considered a major environmental pollutant.
27048796	2	76	theme	chimeric	313:320	arg1	scaffoldin					322:331	a chimeric scaffoldin	311:331	a chimeric scaffoldin that contains 6 cohesins	311:356	The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
27048796	2	76	theme	chimeric	313:320	arg1	cellulosome					279:289	The largest designer cellulosome	258:289	The largest designer cellulosome reported to date	258:306	The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
27048796	12	77	theme	natural	2133:2139	arg1	inefficient					2184:2194	inefficient	2184:2194	inefficient	2184:2194	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	12	77	theme	natural	2133:2139	arg1	process					2163:2169	The natural microbial degradation process	2129:2169	The natural microbial degradation process	2129:2169	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	12	78	theme	biomass	2270:2276	arg1	conversion					2242:2251	the conversion	2238:2251	the conversion of plant-derived biomass to biofuels	2238:2288	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	3	79	theme	specificities-the	610:626	arg1	hallmark					628:635	available cohesin-dockerin specificities-the hallmark	583:635	available cohesin-dockerin specificities-the hallmark of designer cellulosomes	583:660	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	3	80	theme	available	583:591	arg1	hallmark					628:635	available cohesin-dockerin specificities-the hallmark	583:635	available cohesin-dockerin specificities-the hallmark of designer cellulosomes	583:660	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	1	81	theme	recombinant	217:227	arg1	enzymes					249:255	recombinant dockerin-containing enzymes	217:255	recombinant dockerin-containing enzymes	217:255	UNLABELLED Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes.
27048796	6	82	theme	adaptor	1033:1039	arg1	scaffoldin					1041:1050	adaptor scaffoldin	1033:1050	adaptor scaffoldin	1033:1050	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	8	83	theme	enzymatic	1551:1559	arg1	cocktail					1561:1568	a potent enzymatic cocktail	1542:1568	a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates	1542:1625	The resultant extended designer cellulosome comprised 8 recombinant enzymes-4 xylanases and 4 cellulases-thereby representing a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates.
27048796	1	84	theme	chimeric	144:151	arg1	scaffoldins					169:179	chimeric cohesin-bearing scaffoldins	144:179	chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes	144:255	UNLABELLED Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes.
27048796	3	85	theme	cellulosomes	649:660	arg1	hallmark					628:635	available cohesin-dockerin specificities-the hallmark	583:635	available cohesin-dockerin specificities-the hallmark of designer cellulosomes	583:660	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	5	86	theme	various	920:926	arg1	enzymes					928:934	various enzymes	920:934	various enzymes	920:934	Adaptor scaffoldins comprise an intermediate type of scaffoldin that can both incorporate various enzymes and attach to an additional scaffoldin.
27048796	4	87	theme	enzymes	702:708	arg1	number					692:697	the number	688:697	the number of enzymes integrated into designer cellulosomes	688:746	Nevertheless, increasing the number of enzymes integrated into designer cellulosomes is critical, in order to further enhance degradation of plant cell wall material.
27048796	11	88	theme	IMPORTANCE	1967:1976	arg1	residues					1994:2001	IMPORTANCE Plant cell wall residues	1967:2001	IMPORTANCE Plant cell wall residues	1967:2001	IMPORTANCE Plant cell wall residues represent a major source of renewable biomass for the production of biofuels such as ethanol via breakdown to soluble sugars.
27048796	2	89	theme	largest	262:268	arg1	scaffoldin					322:331	a chimeric scaffoldin	311:331	a chimeric scaffoldin that contains 6 cohesins	311:356	The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
27048796	2	89	theme	largest	262:268	arg1	cellulosome					279:289	The largest designer cellulosome	258:289	The largest designer cellulosome reported to date	258:306	The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
27048796	1	90	theme	Designer	111:118	arg1	cellulosomes					120:131	UNLABELLED Designer cellulosomes	100:131	UNLABELLED Designer cellulosomes	100:131	UNLABELLED Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes.
27048796	14	91	theme	wall	2511:2514	arg1	degradation					2516:2526	enhanced plant cell wall degradation	2491:2526	enhanced plant cell wall degradation	2491:2526	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	11	92	theme	soluble	2113:2119	arg1	sugars					2121:2126	soluble sugars	2113:2126	soluble sugars	2113:2126	IMPORTANCE Plant cell wall residues represent a major source of renewable biomass for the production of biofuels such as ethanol via breakdown to soluble sugars.
27048796	8	93	theme	lignocellulosic	1600:1614	arg1	substrates					1616:1625	natural lignocellulosic substrates	1592:1625	natural lignocellulosic substrates	1592:1625	The resultant extended designer cellulosome comprised 8 recombinant enzymes-4 xylanases and 4 cellulases-thereby representing a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates.
27048796	14	94	theme	plant	2500:2504	arg1	degradation					2516:2526	enhanced plant cell wall degradation	2491:2526	enhanced plant cell wall degradation	2491:2526	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	7	95	theme	hexavalent	1290:1299	arg1	scaffoldin					1301:1310	a hexavalent scaffoldin	1288:1310	a hexavalent scaffoldin capable of connecting to the adaptor scaffoldin by the incorporation of an appropriate type II cohesin	1288:1413	In parallel, we designed a hexavalent scaffoldin capable of connecting to the adaptor scaffoldin by the incorporation of an appropriate type II cohesin.
27048796	5	96	theme	additional	953:962	arg1	scaffoldin					964:973	an additional scaffoldin	950:973	an additional scaffoldin	950:973	Adaptor scaffoldins comprise an intermediate type of scaffoldin that can both incorporate various enzymes and attach to an additional scaffoldin.
27048796	15	97	from	breakthrough	2699:2710	arg1	capacity					2719:2726	our capacity to produce extended designer cellulosomes via synthetic biology means	2715:2796	our capacity to produce extended designer cellulosomes via synthetic biology means	2715:2796	The findings reported in this article comprise a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means, thus enabling the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex.
27048796	5	98	theme	Adaptor	830:836	arg1	scaffoldins					838:848	Adaptor scaffoldins	830:848	Adaptor scaffoldins	830:848	Adaptor scaffoldins comprise an intermediate type of scaffoldin that can both incorporate various enzymes and attach to an additional scaffoldin.
27048796	9	99	theme	adaptor	1652:1658	arg1	scaffoldin					1660:1669	the adaptor scaffoldin	1648:1669	the adaptor scaffoldin	1648:1669	The contribution of the adaptor scaffoldin clearly demonstrated that proximity between the two scaffoldins and their composite set of enzymes is crucial for optimized degradation.
27048796	6	100	theme	I	1078:1078	arg1	cohesins					1080:1087	three type I cohesins	1067:1087	three type I cohesins for enzyme integration	1067:1110	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	14	101	theme	cellulosome	2463:2473	arg1	nanodevices					2475:2485	designer cellulosome nanodevices	2454:2485	designer cellulosome nanodevices for enhanced plant cell wall degradation	2454:2526	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	3	102	theme	sorts	408:412	arg1	limit					399:403	a technical limit	387:403	a technical limit of sorts	387:412	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	4	103	theme	material	820:827	arg1	degradation					789:799	degradation	789:799	degradation of plant cell wall material	789:827	Nevertheless, increasing the number of enzymes integrated into designer cellulosomes is critical, in order to further enhance degradation of plant cell wall material.
27048796	10	104	theme	incubation	1822:1831	arg1	h					1817:1817	72 h	1814:1817	72 h of incubation	1814:1831	After 72 h of incubation, the performance of the extended designer cellulosome was determined to be approximately 70% compared to that of native cellulosomes.
27048796	15	105	theme	higher-order	2829:2840	arg1	complexes					2842:2850	higher-order complexes	2829:2850	higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex	2829:2932	The findings reported in this article comprise a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means, thus enabling the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex.
27048796	8	106	theme	resultant	1420:1428	arg1	cellulosome					1448:1458	The resultant extended designer cellulosome	1416:1458	The resultant extended designer cellulosome	1416:1458	The resultant extended designer cellulosome comprised 8 recombinant enzymes-4 xylanases and 4 cellulases-thereby representing a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates.
27048796	3	107	theme	low	483:485	arg1	levels					498:503	resultant low expression levels	473:503	resultant low expression levels	473:503	This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes.
27048796	7	108	theme	appropriate	1387:1397	arg1	cohesin					1407:1413	an appropriate type II cohesin	1384:1413	an appropriate type II cohesin	1384:1413	In parallel, we designed a hexavalent scaffoldin capable of connecting to the adaptor scaffoldin by the incorporation of an appropriate type II cohesin.
27048796	11	109	theme	major	2015:2019	arg1	source					2021:2026	a major source	2013:2026	a major source of renewable biomass for the production of biofuels such as ethanol	2013:2094	IMPORTANCE Plant cell wall residues represent a major source of renewable biomass for the production of biofuels such as ethanol via breakdown to soluble sugars.
27048796	8	110	theme	designer	1439:1446	arg1	cellulosome					1448:1458	The resultant extended designer cellulosome	1416:1458	The resultant extended designer cellulosome	1416:1458	The resultant extended designer cellulosome comprised 8 recombinant enzymes-4 xylanases and 4 cellulases-thereby representing a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates.
27048796	11	111	theme	renewable	2031:2039	arg1	biomass					2041:2047	renewable biomass	2031:2047	renewable biomass	2031:2047	IMPORTANCE Plant cell wall residues represent a major source of renewable biomass for the production of biofuels such as ethanol via breakdown to soluble sugars.
27048796	15	112	theme	single	2907:2912	arg1	complex					2926:2932	a single multienzyme complex	2905:2932	a single multienzyme complex	2905:2932	The findings reported in this article comprise a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means, thus enabling the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex.
27048796	5	113	theme	scaffoldin	883:892	arg1	type					875:878	an intermediate type	859:878	an intermediate type of scaffoldin that can both incorporate various enzymes and attach to an additional scaffoldin	859:973	Adaptor scaffoldins comprise an intermediate type of scaffoldin that can both incorporate various enzymes and attach to an additional scaffoldin.
27048796	6	114	theme	type	1122:1125	arg1	dockerin					1130:1137	a single type II dockerin	1113:1137	a single type II dockerin for interaction with an additional scaffoldin	1113:1183	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	4	115	theme	cell	810:813	arg1	material					820:827	plant cell wall material	804:827	plant cell wall material	804:827	Nevertheless, increasing the number of enzymes integrated into designer cellulosomes is critical, in order to further enhance degradation of plant cell wall material.
27048796	10	116	theme	designer	1866:1873	arg1	cellulosome					1875:1885	the extended designer cellulosome	1853:1885	the extended designer cellulosome	1853:1885	After 72 h of incubation, the performance of the extended designer cellulosome was determined to be approximately 70% compared to that of native cellulosomes.
27048796	1	117	theme	enzymes	249:255	arg1	incorporation					200:212	the controlled incorporation	185:212	the controlled incorporation of recombinant dockerin-containing enzymes	185:255	UNLABELLED Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes.
27048796	11	118	theme	biomass	2041:2047	arg1	source					2021:2026	a major source	2013:2026	a major source of renewable biomass for the production of biofuels such as ethanol	2013:2094	IMPORTANCE Plant cell wall residues represent a major source of renewable biomass for the production of biofuels such as ethanol via breakdown to soluble sugars.
27048796	8	119	theme	recombinant	1472:1482	arg1	xylanases					1494:1502	8 recombinant enzymes-4 xylanases	1470:1502	8 recombinant enzymes-4 xylanases	1470:1502	The resultant extended designer cellulosome comprised 8 recombinant enzymes-4 xylanases and 4 cellulases-thereby representing a potent enzymatic cocktail for solubilization of natural lignocellulosic substrates.
27048796	5	120	theme	intermediate	862:873	arg1	type					875:878	an intermediate type	859:878	an intermediate type of scaffoldin that can both incorporate various enzymes and attach to an additional scaffoldin	859:973	Adaptor scaffoldins comprise an intermediate type of scaffoldin that can both incorporate various enzymes and attach to an additional scaffoldin.
27048796	12	121	theme	degradation	2151:2161	arg1	inefficient					2184:2194	inefficient	2184:2194	inefficient	2184:2194	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	12	121	theme	degradation	2151:2161	arg1	process					2163:2169	The natural microbial degradation process	2129:2169	The natural microbial degradation process	2129:2169	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	9	122	theme	composite	1745:1753	arg1	enzymes					1762:1768	enzymes	1762:1768	enzymes	1762:1768	The contribution of the adaptor scaffoldin clearly demonstrated that proximity between the two scaffoldins and their composite set of enzymes is crucial for optimized degradation.
27048796	9	122	theme	composite	1745:1753	arg1	set					1755:1757	their composite set	1739:1757	their composite set of enzymes	1739:1768	The contribution of the adaptor scaffoldin clearly demonstrated that proximity between the two scaffoldins and their composite set of enzymes is crucial for optimized degradation.
27048796	6	123	theme	carbohydrate-binding	1192:1211	arg1	module					1213:1218	a carbohydrate-binding module	1190:1218	a carbohydrate-binding module for targeting to the cellulosic substrate	1190:1260	Using this strategy, we constructed an efficient form of adaptor scaffoldin that possesses three type I cohesins for enzyme integration, a single type II dockerin for interaction with an additional scaffoldin, and a carbohydrate-binding module for targeting to the cellulosic substrate.
27048796	12	124	theme	cellulosic	2338:2347	arg1	wastes					2349:2354	human-generated cellulosic wastes	2322:2354	human-generated cellulosic wastes	2322:2354	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	14	125	theme	production	2603:2612	arg1	fields					2558:2563	the fields	2554:2563	the fields of environmental pollution, bioenergy production, and biotechnology in general	2554:2642	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	11	126	theme	cell	1984:1987	arg1	residues					1994:2001	IMPORTANCE Plant cell wall residues	1967:2001	IMPORTANCE Plant cell wall residues	1967:2001	IMPORTANCE Plant cell wall residues represent a major source of renewable biomass for the production of biofuels such as ethanol via breakdown to soluble sugars.
27048796	15	127	theme	designer	2748:2755	arg1	cellulosomes					2757:2768	extended designer cellulosomes	2739:2768	extended designer cellulosomes	2739:2768	The findings reported in this article comprise a true breakthrough in our capacity to produce extended designer cellulosomes via synthetic biology means, thus enabling the assembly of higher-order complexes that can supersede the number of enzymes included in a single multienzyme complex.
27048796	12	128	theme	dedicated	2303:2311	arg1	crops					2313:2317	dedicated crops	2303:2317	dedicated crops	2303:2317	The natural microbial degradation process, however, is inefficient for achieving cost-effective processes in the conversion of plant-derived biomass to biofuels, either from dedicated crops or human-generated cellulosic wastes.
27048796	14	129	theme	environmental	2568:2580	arg1	pollution					2582:2590	environmental pollution	2568:2590	environmental pollution	2568:2590	The development of designer cellulosome nanodevices for enhanced plant cell wall degradation thus has major impacts in the fields of environmental pollution, bioenergy production, and biotechnology in general.
27048796	10	130	theme	extended	1857:1864	arg1	cellulosome					1875:1885	the extended designer cellulosome	1853:1885	the extended designer cellulosome	1853:1885	After 72 h of incubation, the performance of the extended designer cellulosome was determined to be approximately 70% compared to that of native cellulosomes.
28971475	13	0	theme	offspring	2009:2017	arg1	composition					2024:2034	young adult offspring body composition	1997:2034	young adult offspring body composition	1997:2034	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	11	1	theme	protein	1612:1618	arg1	B					1627:1627	phosphorylated protein kinase B	1597:1627	phosphorylated protein kinase B	1597:1627	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	7	2	theme	standard	1123:1130	arg1	diet					1132:1135	a standard diet	1121:1135	a standard diet (TRCD or CTCD)	1121:1150	At weaning, pups from each group were fed either a standard diet (TRCD or CTCD) or a high-fat/high-sucrose diet (TRHF or CTHF) for 10 weeks.
28971475	13	3	from	effects	1986:1992	arg1	metabolism					2073:2082	lipid metabolism	2067:2082	lipid metabolism	2067:2082	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	13	3	from	effects	1986:1992	arg1	composition					2024:2034	young adult offspring body composition	1997:2034	young adult offspring body composition	1997:2034	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	13	3	from	effects	1986:1992	arg1	carbohydrate					2050:2061	muscle carbohydrate	2043:2061	muscle carbohydrate	2043:2061	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	5	4	theme	exercise	667:674	arg1	effect					648:653	the effect	644:653	the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle	644:900	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	1	5	theme	body	192:195	arg1	composition					197:207	offspring body composition	182:207	offspring body composition	182:207	KEY POINTS Maternal training during gestation enhances offspring body composition and energy substrates handling in early adulthood.
28971475	6	6	theme	female	932:937	arg1	rats					946:949	Fifteen-week-old nulliparous female Wistar rats	903:949	Fifteen-week-old nulliparous female Wistar rats	903:949	Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT).
28971475	2	7	theme	maternal	328:335	arg1	training					337:344	maternal training	328:344	maternal training	328:344	Offspring nutrition also plays a role as some beneficial effects of maternal training during gestation disappear after consumption of a high-fat diet.
28971475	12	8	theme	CT	1878:1879	arg1	group					1881:1885	the CT group	1874:1885	the CT group (-46% and -58%, respectively, P < 0.05)	1874:1925	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	6	9	theme	submaximal	1018:1027	arg1	TR					1040:1041	TR	1040:1041	TR	1040:1041	Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT).
28971475	6	9	theme	submaximal	1018:1027	arg1	intensity					1029:1037	a constant submaximal intensity	1007:1037	a constant submaximal intensity (TR)	1007:1042	Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT).
28971475	13	10	theme	positive	1977:1984	arg1	effects					1986:1992	positive effects	1977:1992	positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism	1977:2082	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	5	11	dep	composition	781:791	arg1	handling					836:843	handling	836:843	handling	836:843	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	5	12	theme	major	852:856	arg1	liver					885:889	liver	885:889	liver	885:889	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	5	12	theme	major	852:856	arg1	tissues					876:882	two major glucose-utilizing tissues	848:882	two major glucose-utilizing tissues: liver and muscle	848:900	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	5	12	theme	major	852:856	arg1	muscle					895:900	muscle	895:900	muscle	895:900	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	8	13	from	dams	1231:1234	arg1	Offspring					1213:1221	Offspring	1213:1221	Offspring from TR dams	1213:1234	Offspring from TR dams gained less weight compared to those from CT dams.
28971475	12	14	from	rats	1754:1757	arg1	fibres					1739:1744	permeabilized isolated muscle fibres	1709:1744	permeabilized isolated muscle fibres from TR rats	1709:1757	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	1	15	theme	KEY	127:129	arg1	POINTS					131:136	KEY POINTS	127:136	KEY POINTS Maternal training during gestation	127:171	KEY POINTS Maternal training during gestation enhances offspring body composition and energy substrates handling in early adulthood.
28971475	8	16	theme	CT	1278:1279	arg1	dams					1281:1284	CT dams	1278:1284	CT dams	1278:1284	Offspring from TR dams gained less weight compared to those from CT dams.
28971475	5	17	theme	high-fat/high-sucrose	727:747	arg1	HF					755:756	HF	755:756	HF	755:756	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	5	17	theme	high-fat/high-sucrose	727:747	arg1	diet					749:752	a high-fat/high-sucrose diet	725:752	a high-fat/high-sucrose diet (HF)	725:757	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	1	18	theme	Maternal	138:145	arg1	training					147:154	KEY POINTS Maternal training	127:154	KEY POINTS Maternal training during gestation	127:171	KEY POINTS Maternal training during gestation enhances offspring body composition and energy substrates handling in early adulthood.
28971475	11	19	theme	CT	1669:1670	arg1	offspring					1672:1680	CT offspring	1669:1680	CT offspring	1669:1680	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	0	20	theme	male	78:81	arg1	offspring					83:91	male offspring	78:91	male offspring fed a high-fat/high-sucrose diet	78:124	Maternal exercise modifies body composition and energy substrates handling in male offspring fed a high-fat/high-sucrose diet.
28971475	0	21	theme	high-fat/high-sucrose	99:119	arg1	diet					121:124	a high-fat/high-sucrose diet	97:124	a high-fat/high-sucrose diet	97:124	Maternal exercise modifies body composition and energy substrates handling in male offspring fed a high-fat/high-sucrose diet.
28971475	9	22	theme	Selected	1287:1294	arg1	depots					1300:1305	Selected fat depots	1287:1305	Selected fat depots	1287:1305	Selected fat depots were larger with the HF diet compared to control diet (CD) but significantly smaller in TRHF compared to CTHF.
28971475	3	23	theme	ABSTRACT	411:418	arg1	exercise					429:436	ABSTRACT Maternal exercise	411:436	ABSTRACT Maternal exercise during gestation	411:453	ABSTRACT Maternal exercise during gestation has been reported to modify offspring metabolism and health.
28971475	0	24	theme	Maternal	0:7	arg1	exercise					9:16	Maternal exercise	0:16	Maternal exercise	0:16	Maternal exercise modifies body composition and energy substrates handling in male offspring fed a high-fat/high-sucrose diet.
28971475	13	25	contain	has	1973:1975	arg1	exercise					1964:1971	maternal exercise	1955:1971	maternal exercise	1955:1971	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	13	25	contain	has	1973:1975	arg2	effects					1986:1992	positive effects	1977:1992	positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism	1977:2082	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	10	26	theme	secretion	1444:1452	arg1	higher					1464:1469	higher	1464:1469	higher	1464:1469	Surprisingly, the insulin secretion index was higher in islets from HF offspring compared to CD.
28971475	10	26	theme	secretion	1444:1452	arg1	index					1454:1458	the insulin secretion index	1432:1458	the insulin secretion index	1432:1458	Surprisingly, the insulin secretion index was higher in islets from HF offspring compared to CD.
28971475	5	27	theme	glucose-utilizing	858:874	arg1	liver					885:889	liver	885:889	liver	885:889	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	5	27	theme	glucose-utilizing	858:874	arg1	tissues					876:882	two major glucose-utilizing tissues	848:882	two major glucose-utilizing tissues: liver and muscle	848:900	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	5	27	theme	glucose-utilizing	858:874	arg1	muscle					895:900	muscle	895:900	muscle	895:900	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	13	28	theme	nutritional	2101:2111	arg1	status					2113:2118	the nutritional status	2097:2118	the nutritional status	2097:2118	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	6	29	theme	nulliparous	920:930	arg1	rats					946:949	Fifteen-week-old nulliparous female Wistar rats	903:949	Fifteen-week-old nulliparous female Wistar rats	903:949	Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT).
28971475	6	30	from	intensity	1029:1037	arg1	gestation					994:1002	gestation	994:1002	gestation at a constant submaximal intensity (TR)	994:1042	Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT).
28971475	11	31	theme	protein	1638:1644	arg1	B					1653:1653	total protein kinase B	1632:1653	total protein kinase B	1632:1653	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	1	32	dep	composition	197:207	arg1	handling					231:238	handling	231:238	handling	231:238	KEY POINTS Maternal training during gestation enhances offspring body composition and energy substrates handling in early adulthood.
28971475	5	33	theme	energy	818:823	arg1	substrates					825:834	energy substrates	818:834	energy substrates	818:834	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	12	34	theme	apparent	1777:1784	arg1	Km					1805:1806	Km	1805:1806	Km	1805:1806	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	12	34	theme	apparent	1777:1784	arg1	constant					1795:1802	a lower apparent affinity constant	1769:1802	a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates	1769:1860	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	10	35	from	offspring	1489:1497	arg1	islets					1474:1479	islets	1474:1479	islets from HF offspring	1474:1497	Surprisingly, the insulin secretion index was higher in islets from HF offspring compared to CD.
28971475	12	36	theme	isolated	1723:1730	arg1	fibres					1739:1744	permeabilized isolated muscle fibres	1709:1744	permeabilized isolated muscle fibres from TR rats	1709:1757	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	0	37	theme	energy	48:53	arg1	substrates					55:64	energy substrates	48:64	energy substrates	48:64	Maternal exercise modifies body composition and energy substrates handling in male offspring fed a high-fat/high-sucrose diet.
28971475	0	38	dep	composition	32:42	arg1	handling					66:73	handling	66:73	handling	66:73	Maternal exercise modifies body composition and energy substrates handling in male offspring fed a high-fat/high-sucrose diet.
28971475	1	39	theme	early	243:247	arg1	adulthood					249:257	early adulthood	243:257	early adulthood	243:257	KEY POINTS Maternal training during gestation enhances offspring body composition and energy substrates handling in early adulthood.
28971475	5	40	dep	tissues	876:882	arg1	liver					885:889	liver	885:889	liver	885:889	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	5	40	dep	tissues	876:882	arg1	tissues					876:882	two major glucose-utilizing tissues	848:882	two major glucose-utilizing tissues: liver and muscle	848:900	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	5	40	dep	tissues	876:882	arg1	muscle					895:900	muscle	895:900	muscle	895:900	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	2	41	theme	high-fat	396:403	arg1	diet					405:408	a high-fat diet	394:408	a high-fat diet	394:408	Offspring nutrition also plays a role as some beneficial effects of maternal training during gestation disappear after consumption of a high-fat diet.
28971475	13	42	theme	muscle	2043:2048	arg1	carbohydrate					2050:2061	muscle carbohydrate	2043:2061	muscle carbohydrate	2043:2061	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	13	43	theme	lipid	2067:2071	arg1	metabolism					2073:2082	lipid metabolism	2067:2082	lipid metabolism	2067:2082	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	11	44	theme	muscle	1544:1549	arg1	sensitivity					1559:1569	a higher muscle insulin sensitivity	1535:1569	a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B	1535:1653	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	13	45	theme	adult	2003:2007	arg1	composition					2024:2034	young adult offspring body composition	1997:2034	young adult offspring body composition	1997:2034	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	2	46	theme	beneficial	306:315	arg1	effects					317:323	some beneficial effects	301:323	some beneficial effects of maternal training during gestation	301:361	Offspring nutrition also plays a role as some beneficial effects of maternal training during gestation disappear after consumption of a high-fat diet.
28971475	13	47	theme	body	2019:2022	arg1	composition					2024:2034	young adult offspring body composition	1997:2034	young adult offspring body composition	1997:2034	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	11	48	theme	kinase	1620:1625	arg1	B					1627:1627	phosphorylated protein kinase B	1597:1627	phosphorylated protein kinase B	1597:1627	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	5	49	theme	maternal	658:665	arg1	exercise					667:674	maternal exercise	658:674	maternal exercise before and during gestation	658:702	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	1	50	theme	offspring	182:190	arg1	composition					197:207	offspring body composition	182:207	offspring body composition	182:207	KEY POINTS Maternal training during gestation enhances offspring body composition and energy substrates handling in early adulthood.
28971475	6	51	theme	Wistar	939:944	arg1	rats					946:949	Fifteen-week-old nulliparous female Wistar rats	903:949	Fifteen-week-old nulliparous female Wistar rats	903:949	Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT).
28971475	11	52	dep	showed	1528:1533	arg1	P < 0.05					1689:1696	P < 0.05	1689:1696	P < 0.05	1689:1696	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	11	52	dep	showed	1528:1533	arg1	%					1686:1686	+48%	1683:1686	+48%	1683:1686	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	11	53	theme	phosphorylated	1597:1610	arg1	B					1627:1627	phosphorylated protein kinase B	1597:1627	phosphorylated protein kinase B	1597:1627	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	2	54	theme	training	337:344	arg1	effects					317:323	some beneficial effects	301:323	some beneficial effects of maternal training during gestation	301:361	Offspring nutrition also plays a role as some beneficial effects of maternal training during gestation disappear after consumption of a high-fat diet.
28971475	8	55	theme	TR	1228:1229	arg1	dams					1231:1234	TR dams	1228:1234	TR dams	1228:1234	Offspring from TR dams gained less weight compared to those from CT dams.
28971475	7	56	dep	diet	1132:1135	arg1	CTCD					1146:1149	CTCD	1146:1149	CTCD	1146:1149	At weaning, pups from each group were fed either a standard diet (TRCD or CTCD) or a high-fat/high-sucrose diet (TRHF or CTHF) for 10 weeks.
28971475	7	56	dep	diet	1132:1135	arg1	TRCD					1138:1141	TRCD	1138:1141	TRCD	1138:1141	At weaning, pups from each group were fed either a standard diet (TRCD or CTCD) or a high-fat/high-sucrose diet (TRHF or CTHF) for 10 weeks.
28971475	1	57	theme	energy	213:218	arg1	substrates					220:229	energy substrates	213:229	energy substrates	213:229	KEY POINTS Maternal training during gestation enhances offspring body composition and energy substrates handling in early adulthood.
28971475	6	58	theme	constant	1009:1016	arg1	TR					1040:1041	TR	1040:1041	TR	1040:1041	Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT).
28971475	6	58	theme	constant	1009:1016	arg1	intensity					1029:1037	a constant submaximal intensity	1007:1037	a constant submaximal intensity (TR)	1007:1042	Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT).
28971475	2	59	theme	Offspring	260:268	arg1	nutrition					270:278	Offspring nutrition	260:278	Offspring nutrition	260:278	Offspring nutrition also plays a role as some beneficial effects of maternal training during gestation disappear after consumption of a high-fat diet.
28971475	12	60	theme	coenzyme	1837:1844	arg1	A					1846:1846	palmitoyl coenzyme A	1827:1846	palmitoyl coenzyme A	1827:1846	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	13	61	theme	maternal	1955:1962	arg1	exercise					1964:1971	maternal exercise	1955:1971	maternal exercise	1955:1971	These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
28971475	1	62	theme	POINTS	131:136	arg1	training					147:154	KEY POINTS Maternal training	127:154	KEY POINTS Maternal training during gestation	127:171	KEY POINTS Maternal training during gestation enhances offspring body composition and energy substrates handling in early adulthood.
28971475	5	63	from	effect	648:653	arg1	offspring					711:719	the offspring	707:719	the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle	707:900	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	5	64	theme	pancreatic	794:803	arg1	function					805:812	pancreatic function	794:812	pancreatic function	794:812	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	12	65	theme	palmitoyl	1827:1835	arg1	A					1846:1846	palmitoyl coenzyme A	1827:1846	palmitoyl coenzyme A	1827:1846	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	6	66	dep	sedentary	1056:1064	arg1	CT					1067:1068	CT	1067:1068	CT	1067:1068	Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT).
28971475	7	67	dep	diet	1179:1182	arg1	CTHF					1193:1196	CTHF	1193:1196	CTHF	1193:1196	At weaning, pups from each group were fed either a standard diet (TRCD or CTCD) or a high-fat/high-sucrose diet (TRHF or CTHF) for 10 weeks.
28971475	7	67	dep	diet	1179:1182	arg1	TRHF					1185:1188	TRHF	1185:1188	TRHF	1185:1188	At weaning, pups from each group were fed either a standard diet (TRCD or CTCD) or a high-fat/high-sucrose diet (TRHF or CTHF) for 10 weeks.
28971475	7	68	theme	high-fat/high-sucrose	1157:1177	arg1	diet					1179:1182	a high-fat/high-sucrose diet	1155:1182	a high-fat/high-sucrose diet (TRHF or CTHF)	1155:1197	At weaning, pups from each group were fed either a standard diet (TRCD or CTCD) or a high-fat/high-sucrose diet (TRHF or CTHF) for 10 weeks.
28971475	9	69	theme	fat	1296:1298	arg1	depots					1300:1305	Selected fat depots	1287:1305	Selected fat depots	1287:1305	Selected fat depots were larger with the HF diet compared to control diet (CD) but significantly smaller in TRHF compared to CTHF.
28971475	3	70	theme	Maternal	420:427	arg1	exercise					429:436	ABSTRACT Maternal exercise	411:436	ABSTRACT Maternal exercise during gestation	411:453	ABSTRACT Maternal exercise during gestation has been reported to modify offspring metabolism and health.
28971475	7	71	from	group	1099:1103	arg1	pups					1084:1087	pups	1084:1087	pups from each group	1084:1103	At weaning, pups from each group were fed either a standard diet (TRCD or CTCD) or a high-fat/high-sucrose diet (TRHF or CTHF) for 10 weeks.
28971475	2	72	theme	diet	405:408	arg1	consumption					379:389	consumption	379:389	consumption of a high-fat diet	379:408	Offspring nutrition also plays a role as some beneficial effects of maternal training during gestation disappear after consumption of a high-fat diet.
28971475	0	73	theme	body	27:30	arg1	composition					32:42	body composition	27:42	body composition	27:42	Maternal exercise modifies body composition and energy substrates handling in male offspring fed a high-fat/high-sucrose diet.
28971475	12	74	theme	lower	1771:1775	arg1	Km					1805:1806	Km	1805:1806	Km	1805:1806	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	12	74	theme	lower	1771:1775	arg1	constant					1795:1802	a lower apparent affinity constant	1769:1802	a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates	1769:1860	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	10	75	theme	insulin	1436:1442	arg1	higher					1464:1469	higher	1464:1469	higher	1464:1469	Surprisingly, the insulin secretion index was higher in islets from HF offspring compared to CD.
28971475	10	75	theme	insulin	1436:1442	arg1	index					1454:1458	the insulin secretion index	1432:1458	the insulin secretion index	1432:1458	Surprisingly, the insulin secretion index was higher in islets from HF offspring compared to CD.
28971475	11	76	theme	total	1632:1636	arg1	B					1653:1653	total protein kinase B	1632:1653	total protein kinase B	1632:1653	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	12	77	theme	affinity	1786:1793	arg1	Km					1805:1806	Km	1805:1806	Km	1805:1806	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	12	77	theme	affinity	1786:1793	arg1	constant					1795:1802	a lower apparent affinity constant	1769:1802	a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates	1769:1860	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	6	78	theme	Fifteen-week-old	903:918	arg1	rats					946:949	Fifteen-week-old nulliparous female Wistar rats	903:949	Fifteen-week-old nulliparous female Wistar rats	903:949	Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT).
28971475	12	79	dep	group	1881:1885	arg1	P < 0.05					1917:1924	P < 0.05	1917:1924	P < 0.05	1917:1924	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	12	79	dep	group	1881:1885	arg1	%					1891:1891	-46%	1888:1891	-46%	1888:1891	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	12	79	dep	group	1881:1885	arg1	%					1900:1900	-58%	1897:1900	-58%	1897:1900	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	11	80	theme	B	1627:1627	arg1	ratio					1588:1592	the ratio	1584:1592	the ratio of phosphorylated protein kinase B to total protein kinase B	1584:1653	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	5	81	theme	body	776:779	arg1	composition					781:791	its body composition	772:791	its body composition	772:791	Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle.
28971475	9	82	theme	HF	1328:1329	arg1	diet					1331:1334	the HF diet	1324:1334	the HF diet compared to control diet (CD)	1324:1364	Selected fat depots were larger with the HF diet compared to control diet (CD) but significantly smaller in TRHF compared to CTHF.
28971475	12	83	theme	TR	1751:1752	arg1	rats					1754:1757	TR rats	1751:1757	TR rats	1751:1757	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	12	84	theme	permeabilized	1709:1721	arg1	fibres					1739:1744	permeabilized isolated muscle fibres	1709:1744	permeabilized isolated muscle fibres from TR rats	1709:1757	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	4	85	theme	high-fat	585:592	arg1	diet					594:597	a high-fat diet	583:597	a high-fat diet	583:597	Whether these effects are exacerbated when offspring are receiving a high-fat diet remains unclear.
28971475	11	86	theme	TR	1515:1516	arg1	offspring					1518:1526	TR offspring	1515:1526	TR offspring	1515:1526	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	3	87	dep	offspring	483:491	arg1	metabolism					493:502	metabolism	493:502	metabolism	493:502	ABSTRACT Maternal exercise during gestation has been reported to modify offspring metabolism and health.
28971475	12	88	theme	muscle	1732:1737	arg1	fibres					1739:1744	permeabilized isolated muscle fibres	1709:1744	permeabilized isolated muscle fibres from TR rats	1709:1757	With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05).
28971475	11	89	theme	kinase	1646:1651	arg1	B					1653:1653	total protein kinase B	1632:1653	total protein kinase B	1632:1653	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	11	90	theme	insulin	1551:1557	arg1	sensitivity					1559:1569	a higher muscle insulin sensitivity	1535:1569	a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B	1535:1653	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
28971475	9	91	theme	control	1348:1354	arg1	diet					1356:1359	control diet	1348:1359	control diet (CD)	1348:1364	Selected fat depots were larger with the HF diet compared to control diet (CD) but significantly smaller in TRHF compared to CTHF.
28971475	9	91	theme	control	1348:1354	arg1	CD					1362:1363	CD	1362:1363	CD	1362:1363	Selected fat depots were larger with the HF diet compared to control diet (CD) but significantly smaller in TRHF compared to CTHF.
28971475	6	92	dep	exercised	951:959	arg1	gestation					994:1002	gestation	994:1002	gestation at a constant submaximal intensity (TR)	994:1042	Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT).
28971475	10	93	theme	HF	1486:1487	arg1	offspring					1489:1497	HF offspring	1486:1497	HF offspring	1486:1497	Surprisingly, the insulin secretion index was higher in islets from HF offspring compared to CD.
28971475	11	94	theme	higher	1537:1542	arg1	sensitivity					1559:1569	a higher muscle insulin sensitivity	1535:1569	a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B	1535:1653	TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05).
27231729	3	0	theme	cellulose	637:645	arg1	Regeneration					621:632	Regeneration	621:632	Regeneration of cellulose from printed samples using water	621:678	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	2	1	theme	printability	541:552	arg1	<					564:564	printability parameter < 10	541:567	printability parameter < 10	541:567	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	1	theme	printability	541:552	arg1	print-head					524:533	a DIMATIX print-head	514:533	a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility	514:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	3	2	theme	printed	652:658	arg1	samples					660:666	printed samples	652:666	printed samples using water	652:678	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	3	3	theme	light	817:821	arg1	WLI					839:841	WLI	839:841	WLI	839:841	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	3	3	theme	light	817:821	arg1	interferometry					823:836	white light interferometry	811:836	white light interferometry (WLI)	811:842	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	1	4	theme	dissolution	231:241	arg1	printing					255:262	the dissolution and ink-jet printing	227:262	the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%	227:295	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	1	5	from	%	295:295	arg1	printing					255:262	the dissolution and ink-jet printing	227:262	the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%	227:295	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	1	6	dep	acetate	116:122	arg1	[C2C1Im					125:131	[C2C1Im	125:131	[C2C1Im	125:131	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	3	7	theme	scanning	772:779	arg1	SEM					802:804	SEM	802:804	SEM	802:804	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	3	7	theme	scanning	772:779	arg1	microscopy					790:799	scanning electron microscopy	772:799	scanning electron microscopy (SEM)	772:805	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	2	8	theme	producing	466:474	arg1	samples					476:482	41-47 wt% producing samples	456:482	41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility	456:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	1	9	dep	acetate	172:178	arg1	[C4C1Im					181:187	[C4C1Im	181:187	[C4C1Im	181:187	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	4	10	theme	composite	991:999	arg1	materials					1001:1009	single-component and composite materials	970:1009	single-component and composite materials	970:1009	These results indicate the potential of biorenewable materials to be used in the 3D additive manufacture process to generate single-component and composite materials.
27231729	1	11	theme	ink-jet	247:253	arg1	printing					255:262	the dissolution and ink-jet printing	227:262	the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%	227:295	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	2	12	theme	%	464:464	arg1	samples					476:482	41-47 wt% producing samples	456:482	41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility	456:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	1	13	theme	co-solvents	313:323	arg1	1-butanol					325:333	the co-solvents 1-butanol	309:333	the co-solvents 1-butanol	309:333	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	2	14	theme	print-head	524:533	arg1	range					505:509	the printable range	491:509	the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility	491:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	15	theme	wt	462:463	arg1	samples					476:482	41-47 wt% producing samples	456:482	41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility	456:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	4	16	theme	single-component	970:985	arg1	materials					1001:1009	single-component and composite materials	970:1009	single-component and composite materials	970:1009	These results indicate the potential of biorenewable materials to be used in the 3D additive manufacture process to generate single-component and composite materials.
27231729	2	17	from	DMSO	435:438	arg1	range					447:451	the range	443:451	the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility	443:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	18	dep	used	535:538	arg1	<					564:564	printability parameter < 10	541:567	printability parameter < 10	541:567	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	18	dep	used	535:538	arg1	print-head					524:533	a DIMATIX print-head	514:533	a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility	514:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	19	theme	consistent	409:418	arg1	printing					420:427	consistent printing	409:427	consistent printing	409:427	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	4	20	theme	biorenewable	885:896	arg1	materials					898:906	biorenewable materials	885:906	biorenewable materials	885:906	These results indicate the potential of biorenewable materials to be used in the 3D additive manufacture process to generate single-component and composite materials.
27231729	0	21	theme	dimensional	6:16	arg1	printing					26:33	Three dimensional ink-jet printing	0:33	Three dimensional ink-jet printing of biomaterials	0:49	Three dimensional ink-jet printing of biomaterials using ionic liquids and co-solvents.
27231729	1	22	dep	4.8	289:291	arg1	to					286:287	to	286:287	to	286:287	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	2	23	theme	cellulose	599:607	arg1	solubility					609:618	cellulose solubility	599:618	cellulose solubility	599:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	1	24	theme	1-Ethyl-3-methylimidazolium	88:114	arg1	acetate					116:122	1-Ethyl-3-methylimidazolium acetate	88:122	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc])	88:138	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	1	24	theme	1-Ethyl-3-methylimidazolium	88:114	arg1	acetate					172:178	1-butyl-3-methylimidazolium acetate	144:178	1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc])	144:194	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	1	24	theme	1-Ethyl-3-methylimidazolium	88:114	arg1	solvents					214:221	solvents	214:221	solvents	214:221	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	4	25	theme	manufacture	938:948	arg1	process					950:956	the 3D additive manufacture process	922:956	the 3D additive manufacture process	922:956	These results indicate the potential of biorenewable materials to be used in the 3D additive manufacture process to generate single-component and composite materials.
27231729	2	26	theme	parameter	554:562	arg1	<					564:564	printability parameter < 10	541:567	printability parameter < 10	541:567	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	26	theme	parameter	554:562	arg1	print-head					524:533	a DIMATIX print-head	514:533	a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility	514:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	4	27	theme	additive	929:936	arg1	process					950:956	the 3D additive manufacture process	922:956	the 3D additive manufacture process	922:956	These results indicate the potential of biorenewable materials to be used in the 3D additive manufacture process to generate single-component and composite materials.
27231729	3	28	from	samples	660:666	arg1	Regeneration					621:632	Regeneration	621:632	Regeneration of cellulose from printed samples using water	621:678	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	1	29	theme	cellulose	267:275	arg1	printing					255:262	the dissolution and ink-jet printing	227:262	the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%	227:295	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	2	30	theme	printable	495:503	arg1	range					505:509	the printable range	491:509	the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility	491:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	31	theme	rheological	377:387	arg1	modifiers					389:397	rheological modifiers	377:397	rheological modifiers	377:397	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	31	theme	rheological	377:387	arg1	DMSO					359:362	DMSO	359:362	DMSO	359:362	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	31	theme	rheological	377:387	arg1	1-Butanol					345:353	1-Butanol	345:353	1-Butanol	345:353	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	0	32	theme	ink-jet	18:24	arg1	printing					26:33	Three dimensional ink-jet printing	0:33	Three dimensional ink-jet printing of biomaterials	0:49	Three dimensional ink-jet printing of biomaterials using ionic liquids and co-solvents.
27231729	0	33	theme	biomaterials	38:49	arg1	printing					26:33	Three dimensional ink-jet printing	0:33	Three dimensional ink-jet printing of biomaterials	0:49	Three dimensional ink-jet printing of biomaterials using ionic liquids and co-solvents.
27231729	1	34	used	used	206:209	arg2	acetate					116:122	1-Ethyl-3-methylimidazolium acetate	88:122	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc])	88:138	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	1	34	used	used	206:209	arg2	acetate					172:178	1-butyl-3-methylimidazolium acetate	144:178	1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc])	144:194	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	1	34	used	used	206:209	arg2	solvents					214:221	solvents	214:221	solvents	214:221	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	3	35	theme	resulting	707:715	arg1	changes					728:734	the resulting structural changes	703:734	the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI)	703:842	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	2	36	theme	samples	476:482	arg1	range					447:451	the range	443:451	the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility	443:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	0	37	theme	ionic	57:61	arg1	liquids					63:69	liquids	63:69	liquids	63:69	Three dimensional ink-jet printing of biomaterials using ionic liquids and co-solvents.
27231729	3	38	theme	structural	717:726	arg1	changes					728:734	the resulting structural changes	703:734	the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI)	703:842	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	2	39	theme	DIMATIX	516:522	arg1	<					564:564	printability parameter < 10	541:567	printability parameter < 10	541:567	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	39	theme	DIMATIX	516:522	arg1	print-head					524:533	a DIMATIX print-head	514:533	a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility	514:618	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	1	40	dep	[C4C1Im	181:187	arg1	[OAc					189:192	[OAc	189:192	[OAc	189:192	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	2	41	used	used	369:372	arg2	modifiers					389:397	rheological modifiers	377:397	rheological modifiers	377:397	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	41	used	used	369:372	arg2	DMSO					359:362	DMSO	359:362	DMSO	359:362	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	2	41	used	used	369:372	arg2	1-Butanol					345:353	1-Butanol	345:353	1-Butanol	345:353	1-Butanol and DMSO were used as rheological modifiers to ensure consistent printing, with DMSO in the range of 41-47 wt% producing samples within the printable range of a DIMATIX print-head used (printability parameter < 10) at 55 °C, whilst maintaining cellulose solubility.
27231729	4	42	theme	3D	926:927	arg1	process					950:956	the 3D additive manufacture process	922:956	the 3D additive manufacture process	922:956	These results indicate the potential of biorenewable materials to be used in the 3D additive manufacture process to generate single-component and composite materials.
27231729	1	43	theme	wt	293:294	arg1	%					295:295	1.0 to 4.8 wt%	282:295	1.0 to 4.8 wt%	282:295	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	1	44	dep	[C2C1Im	125:131	arg1	[OAc					133:136	[OAc	133:136	[OAc	133:136	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	3	45	theme	electron	781:788	arg1	SEM					802:804	SEM	802:804	SEM	802:804	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	3	45	theme	electron	781:788	arg1	microscopy					790:799	scanning electron microscopy	772:799	scanning electron microscopy (SEM)	772:805	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	3	46	theme	cellulose	743:751	arg1	sample					753:758	the cellulose sample	739:758	the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI)	739:842	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	4	47	theme	materials	898:906	arg1	potential					872:880	the potential	868:880	the potential of biorenewable materials to be used in the 3D additive manufacture process to generate single-component and composite materials	868:1009	These results indicate the potential of biorenewable materials to be used in the 3D additive manufacture process to generate single-component and composite materials.
27231729	1	48	theme	1-butyl-3-methylimidazolium	144:170	arg1	acetate					116:122	1-Ethyl-3-methylimidazolium acetate	88:122	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc])	88:138	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	1	48	theme	1-butyl-3-methylimidazolium	144:170	arg1	acetate					172:178	1-butyl-3-methylimidazolium acetate	144:178	1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc])	144:194	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	1	48	theme	1-butyl-3-methylimidazolium	144:170	arg1	solvents					214:221	solvents	214:221	solvents	214:221	1-Ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and 1-butyl-3-methylimidazolium acetate ([C4C1Im][OAc]) have been used as solvents for the dissolution and ink-jet printing of cellulose from 1.0 to 4.8 wt%, mixed with the co-solvents 1-butanol and DMSO.
27231729	3	49	theme	white	811:815	arg1	WLI					839:841	WLI	839:841	WLI	839:841	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
27231729	3	49	theme	white	811:815	arg1	interferometry					823:836	white light interferometry	811:836	white light interferometry (WLI)	811:842	Regeneration of cellulose from printed samples using water was demonstrated, with the resulting structural changes to the cellulose sample assessed by scanning electron microscopy (SEM) and white light interferometry (WLI).
24771649	2	0	theme	riboflavin	546:555	arg1	photoinitiator					557:570	a riboflavin photoinitiator	544:570	a riboflavin photoinitiator under blue light	544:587	In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light.
24771649	5	1	theme	bone	1290:1293	arg1	regeneration					1295:1306	bone regeneration	1290:1306	bone regeneration	1290:1306	Similarly, when cells were encapsulated within hydrogels, BMSCs exhibited greater proliferation, ALP activity and mineral deposits in the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration.
24771649	6	2	dep	&	1337:1337	arg1	Sons					1339:1342	Sons	1339:1342	Sons	1339:1342	Copyright © 2014 John Wiley & Sons, Ltd.
24771649	4	3	theme	increased	1021:1029	arg1	mineralization					1031:1044	increased mineralization	1021:1044	upregulated alkaline phosphatase (ALP) activity as well as increased mineralization	962:1044	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	4	4	theme	upregulated	962:972	arg1	activity					1001:1008	upregulated alkaline phosphatase (ALP) activity	962:1008	upregulated alkaline phosphatase (ALP) activity as well as increased mineralization	962:1044	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	1	5	theme	Photopolymerizable	76:93	arg1	hydrogels					95:103	Photopolymerizable hydrogels	76:103	Photopolymerizable hydrogels derived from naturally occurring polymers	76:145	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	5	6	dep	Col.	1197:1200	arg1	demonstrate					1217:1227	demonstrate	1217:1227	demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration	1217:1306	Similarly, when cells were encapsulated within hydrogels, BMSCs exhibited greater proliferation, ALP activity and mineral deposits in the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration.
24771649	4	7	theme	marrow	859:864	arg1	BMSCs					881:885	BMSCs	881:885	BMSCs	881:885	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	4	7	theme	marrow	859:864	arg1	cells					874:878	mouse bone marrow stromal cells	848:878	mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels	848:910	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	4	8	theme	MeGC	931:934	arg1	hydrogels					936:944	pure MeGC hydrogels	926:944	pure MeGC hydrogels	926:944	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	2	9	theme	methacrylated	461:473	arg1	chitosan					482:489	photocrosslinkable methacrylated glycol chitosan	442:489	photocrosslinkable methacrylated glycol chitosan (MeGC)	442:496	In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light.
24771649	2	9	theme	methacrylated	461:473	arg1	MeGC					492:495	MeGC	492:495	MeGC	492:495	In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light.
24771649	2	10	theme	blue	578:581	arg1	light					583:587	blue light	578:587	blue light	578:587	In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light.
24771649	4	11	theme	pure	926:929	arg1	hydrogels					936:944	pure MeGC hydrogels	926:944	pure MeGC hydrogels	926:944	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	1	12	from	interest	174:181	arg1	applications					205:216	tissue-engineering applications	186:216	tissue-engineering applications	186:216	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	2	13	theme	semi-interpenetrating	502:522	arg1	Col					534:536	Col	534:536	Col	534:536	In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light.
24771649	2	13	theme	semi-interpenetrating	502:522	arg1	collagen					524:531	semi-interpenetrating collagen	502:531	semi-interpenetrating collagen (Col)	502:537	In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light.
24771649	1	14	theme	excellent	231:239	arg1	biocompatibility					241:256	their excellent biocompatibility	225:256	their excellent biocompatibility	225:256	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	1	14	theme	excellent	231:239	arg1	nature					271:276	hydrophilic nature	259:276	hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure	259:376	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	5	15	theme	Col.	1197:1200	arg1	presence					1185:1192	the presence	1181:1192	the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration	1181:1306	Similarly, when cells were encapsulated within hydrogels, BMSCs exhibited greater proliferation, ALP activity and mineral deposits in the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration.
24771649	4	16	theme	composite	725:733	arg1	hydrogels					735:743	MeGC-Col composite hydrogels	716:743	MeGC-Col composite hydrogels	716:743	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	0	17	theme	Photopolymerizable	0:17	arg1	chitosan-collagen					19:35	Photopolymerizable chitosan-collagen	0:35	Photopolymerizable chitosan-collagen	0:35	Photopolymerizable chitosan-collagen hydrogels for bone tissue engineering.
24771649	1	18	theme	occurring	128:136	arg1	polymers					138:145	naturally occurring polymers	118:145	naturally occurring polymers	118:145	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	4	19	theme	stromal	866:872	arg1	BMSCs					881:885	BMSCs	881:885	BMSCs	881:885	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	4	19	theme	stromal	866:872	arg1	cells					874:878	mouse bone marrow stromal cells	848:878	mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels	848:910	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	5	20	theme	mineral	1161:1167	arg1	deposits					1169:1176	mineral deposits	1161:1176	mineral deposits	1161:1176	Similarly, when cells were encapsulated within hydrogels, BMSCs exhibited greater proliferation, ALP activity and mineral deposits in the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration.
24771649	3	21	theme	MeGC	618:621	arg1	hydrogels					623:631	MeGC hydrogels	618:631	MeGC hydrogels	618:631	The incorporation of Col in MeGC hydrogels enhanced the compressive modulus and slowed the degradation rate of the hydrogels.
24771649	1	22	theme	hydrophilic	259:269	arg1	biocompatibility					241:256	their excellent biocompatibility	225:256	their excellent biocompatibility	225:256	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	1	22	theme	hydrophilic	259:269	arg1	nature					271:276	hydrophilic nature	259:276	hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure	259:376	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	4	23	theme	mouse	848:852	arg1	BMSCs					881:885	BMSCs	881:885	BMSCs	881:885	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	4	23	theme	mouse	848:852	arg1	cells					874:878	mouse bone marrow stromal cells	848:878	mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels	848:910	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	0	24	theme	tissue	56:61	arg1	engineering					63:73	bone tissue engineering	51:73	bone tissue engineering	51:73	Photopolymerizable chitosan-collagen hydrogels for bone tissue engineering.
24771649	1	25	attach	derived	105:111	arg1	polymers					138:145	naturally occurring polymers	118:145	naturally occurring polymers	118:145	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	1	25	attach	derived	105:111	arg2	hydrogels					95:103	Photopolymerizable hydrogels	76:103	Photopolymerizable hydrogels derived from naturally occurring polymers	76:145	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	4	26	theme	MeGC-Col	716:723	arg1	hydrogels					735:743	MeGC-Col composite hydrogels	716:743	MeGC-Col composite hydrogels	716:743	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	1	27	theme	favourable	278:287	arg1	biocompatibility					241:256	their excellent biocompatibility	225:256	their excellent biocompatibility	225:256	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	1	27	theme	favourable	278:287	arg1	nature					271:276	hydrophilic nature	259:276	hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure	259:376	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	0	28	theme	bone	51:54	arg1	engineering					63:73	bone tissue engineering	51:73	bone tissue engineering	51:73	Photopolymerizable chitosan-collagen hydrogels for bone tissue engineering.
24771649	1	29	theme	invasive	359:366	arg1	procedure					368:376	a minimally invasive procedure	347:376	a minimally invasive procedure	347:376	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	2	30	theme	glycol	475:480	arg1	chitosan					482:489	photocrosslinkable methacrylated glycol chitosan	442:489	photocrosslinkable methacrylated glycol chitosan (MeGC)	442:496	In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light.
24771649	2	30	theme	glycol	475:480	arg1	MeGC					492:495	MeGC	492:495	MeGC	492:495	In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light.
24771649	5	31	theme	MeGC-Col	1234:1241	arg1	hydrogels					1253:1261	MeGC-Col composite hydrogels	1234:1261	MeGC-Col composite hydrogels	1234:1261	Similarly, when cells were encapsulated within hydrogels, BMSCs exhibited greater proliferation, ALP activity and mineral deposits in the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration.
24771649	4	32	theme	phosphatase	983:993	arg1	activity					1001:1008	upregulated alkaline phosphatase (ALP) activity	962:1008	upregulated alkaline phosphatase (ALP) activity as well as increased mineralization	962:1044	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	3	33	theme	hydrogels	705:713	arg1	rate					693:696	the degradation rate	677:696	the degradation rate of the hydrogels	677:713	The incorporation of Col in MeGC hydrogels enhanced the compressive modulus and slowed the degradation rate of the hydrogels.
24771649	3	34	theme	compressive	646:656	arg1	modulus					658:664	the compressive modulus	642:664	the compressive modulus	642:664	The incorporation of Col in MeGC hydrogels enhanced the compressive modulus and slowed the degradation rate of the hydrogels.
24771649	4	35	theme	alkaline	974:981	arg1	ALP					996:998	ALP	996:998	ALP	996:998	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	4	35	theme	alkaline	974:981	arg1	phosphatase					983:993	alkaline phosphatase	974:993	upregulated alkaline phosphatase (ALP) activity as well as increased mineralization	962:1044	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	1	36	theme	significant	162:172	arg1	interest					174:181	significant interest	162:181	significant interest in tissue-engineering applications	162:216	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	4	37	theme	osteogenic	818:827	arg1	differentiation					829:843	osteogenic differentiation	818:843	osteogenic differentiation	818:843	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	4	38	theme	bone	854:857	arg1	BMSCs					881:885	BMSCs	881:885	BMSCs	881:885	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	4	38	theme	bone	854:857	arg1	cells					874:878	mouse bone marrow stromal cells	848:878	mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels	848:910	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	1	39	theme	cell	293:296	arg1	ingrowth					298:305	cell ingrowth	293:305	cell ingrowth	293:305	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	4	40	theme	cells	874:878	arg1	proliferation					800:812	proliferation	800:812	proliferation	800:812	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	4	40	theme	cells	874:878	arg1	differentiation					829:843	osteogenic differentiation	818:843	osteogenic differentiation	818:843	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	4	40	theme	cells	874:878	arg1	spreading					789:797	spreading	789:797	spreading	789:797	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	4	40	theme	cells	874:878	arg1	attachment					777:786	cellular attachment	768:786	cellular attachment	768:786	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	5	41	theme	ALP	1144:1146	arg1	activity					1148:1155	ALP activity	1144:1155	ALP activity	1144:1155	Similarly, when cells were encapsulated within hydrogels, BMSCs exhibited greater proliferation, ALP activity and mineral deposits in the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration.
24771649	3	42	theme	Col	611:613	arg1	incorporation					594:606	The incorporation	590:606	The incorporation of Col in MeGC hydrogels	590:631	The incorporation of Col in MeGC hydrogels enhanced the compressive modulus and slowed the degradation rate of the hydrogels.
24771649	4	43	theme	cellular	768:775	arg1	attachment					777:786	cellular attachment	768:786	cellular attachment	768:786	MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization.
24771649	1	44	theme	tissue-engineering	186:203	arg1	applications					205:216	tissue-engineering applications	186:216	tissue-engineering applications	186:216	Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure.
24771649	2	45	theme	photocrosslinkable	442:459	arg1	chitosan					482:489	photocrosslinkable methacrylated glycol chitosan	442:489	photocrosslinkable methacrylated glycol chitosan (MeGC)	442:496	In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light.
24771649	2	45	theme	photocrosslinkable	442:459	arg1	MeGC					492:495	MeGC	492:495	MeGC	492:495	In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light.
24771649	5	46	dep	greater	1121:1127	arg1	proliferation					1129:1141	proliferation	1129:1141	proliferation	1129:1141	Similarly, when cells were encapsulated within hydrogels, BMSCs exhibited greater proliferation, ALP activity and mineral deposits in the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration.
24771649	5	46	dep	greater	1121:1127	arg1	activity					1148:1155	ALP activity	1144:1155	ALP activity	1144:1155	Similarly, when cells were encapsulated within hydrogels, BMSCs exhibited greater proliferation, ALP activity and mineral deposits in the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration.
24771649	5	46	dep	greater	1121:1127	arg1	deposits					1169:1176	mineral deposits	1161:1176	mineral deposits	1161:1176	Similarly, when cells were encapsulated within hydrogels, BMSCs exhibited greater proliferation, ALP activity and mineral deposits in the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration.
24771649	3	47	theme	degradation	681:691	arg1	rate					693:696	the degradation rate	677:696	the degradation rate of the hydrogels	677:713	The incorporation of Col in MeGC hydrogels enhanced the compressive modulus and slowed the degradation rate of the hydrogels.
24771649	2	48	theme	composite	409:417	arg1	hydrogel					419:426	a composite hydrogel	407:426	a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col)	407:537	In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light.
24771649	3	49	from	incorporation	594:606	arg1	hydrogels					623:631	MeGC hydrogels	618:631	MeGC hydrogels	618:631	The incorporation of Col in MeGC hydrogels enhanced the compressive modulus and slowed the degradation rate of the hydrogels.
24771649	5	50	theme	composite	1243:1251	arg1	hydrogels					1253:1261	MeGC-Col composite hydrogels	1234:1261	MeGC-Col composite hydrogels	1234:1261	Similarly, when cells were encapsulated within hydrogels, BMSCs exhibited greater proliferation, ALP activity and mineral deposits in the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration.
26057110	4	0	theme	antimicrobial	944:956	arg1	property					958:965	the antimicrobial property	940:965	the antimicrobial property of the films	940:978	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	1	1	contain	containing	159:168	arg2	compounds					182:190	all natural compounds	170:190	all natural compounds	170:190	Edible antimicrobial composite films from micro-emulsions containing all natural compounds were developed and their antimicrobial properties and microstructures were investigated.
26057110	1	1	contain	containing	159:168	arg1	micro-emulsions					143:157	micro-emulsions	143:157	micro-emulsions containing all natural compounds	143:190	Edible antimicrobial composite films from micro-emulsions containing all natural compounds were developed and their antimicrobial properties and microstructures were investigated.
26057110	4	2	theme	meat	874:877	arg1	samples					879:885	meat samples	874:885	meat samples after 35days at 10°C. AIT	874:911	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	3	theme	soy	719:721	arg1	TSB					730:732	TSB	730:732	TSB	730:732	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	3	theme	soy	719:721	arg1	broth					723:727	tryptic soy broth	711:727	tryptic soy broth (TSB)	711:733	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	4	from	2days	853:857	arg1	samples					879:885	meat samples	874:885	meat samples after 35days at 10°C. AIT	874:911	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	5	theme	additional	1156:1165	arg1	effects					1181:1187	additional antimicrobial effects	1156:1187	additional antimicrobial effects	1156:1187	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	2	6	theme	acetic	372:377	arg1	acids					365:369	organic acids	357:369	organic acids (acetic, lactic and levulinic acids)	357:406	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	6	theme	acetic	372:377	arg1	acids					401:405	acetic, lactic and levulinic acids	372:405	acetic, lactic and levulinic acids	372:405	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	4	7	dep	%	1083:1083	arg1	to					1085:1086	to	1085:1086	to	1085:1086	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	8	from	addition	1095:1102	arg1	%					1089:1089	1.5% to 3%	1080:1089	1.5% to 3%	1080:1089	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	9	theme	ready-to-eat	757:768	arg1	samples					781:787	ready-to-eat (RTE) meat samples	757:787	ready-to-eat (RTE) meat samples	757:787	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	10	theme	antimicrobial	1020:1032	arg1	efficacy					1034:1041	its antimicrobial efficacy	1016:1041	its antimicrobial efficacy	1016:1041	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	2	11	theme	straw	326:330	arg1	BSAX					346:349	BSAX	346:349	BSAX	346:349	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	11	theme	straw	326:330	arg1	Chitosan					281:288	Chitosan	281:288	Chitosan	281:288	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	11	theme	straw	326:330	arg1	agent					455:459	antimicrobial agent	441:459	antimicrobial agent	441:459	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	11	theme	straw	326:330	arg1	arabinoxylan					332:343	barley straw arabinoxylan	319:343	barley straw arabinoxylan (BSAX)	319:350	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	11	theme	straw	326:330	arg1	emulsifier					462:471	emulsifier	462:471	emulsifier	462:471	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	11	theme	straw	326:330	arg1	agent					434:438	film-forming agent	421:438	film-forming agent	421:438	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	11	theme	straw	326:330	arg1	acids					365:369	organic acids	357:369	organic acids (acetic, lactic and levulinic acids)	357:406	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	11	theme	straw	326:330	arg1	isothiocyanate					297:310	allyl isothiocyanate	291:310	allyl isothiocyanate (AIT)	291:316	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	11	theme	straw	326:330	arg1	solvent					478:484	solvent	478:484	solvent	478:484	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	4	12	from	AIT	909:911	arg1	35days					893:898	35days	893:898	35days at 10°C. AIT	893:911	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	13	theme	major	919:923	arg1	contributor					925:935	a major contributor	917:935	a major contributor to the antimicrobial property of the films	917:978	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	14	theme	antimicrobial	1167:1179	arg1	effects					1181:1187	additional antimicrobial effects	1156:1187	additional antimicrobial effects	1156:1187	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	2	15	theme	barley	319:324	arg1	BSAX					346:349	BSAX	346:349	BSAX	346:349	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	15	theme	barley	319:324	arg1	Chitosan					281:288	Chitosan	281:288	Chitosan	281:288	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	15	theme	barley	319:324	arg1	agent					455:459	antimicrobial agent	441:459	antimicrobial agent	441:459	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	15	theme	barley	319:324	arg1	arabinoxylan					332:343	barley straw arabinoxylan	319:343	barley straw arabinoxylan (BSAX)	319:350	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	15	theme	barley	319:324	arg1	emulsifier					462:471	emulsifier	462:471	emulsifier	462:471	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	15	theme	barley	319:324	arg1	agent					434:438	film-forming agent	421:438	film-forming agent	421:438	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	15	theme	barley	319:324	arg1	acids					365:369	organic acids	357:369	organic acids (acetic, lactic and levulinic acids)	357:406	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	15	theme	barley	319:324	arg1	isothiocyanate					297:310	allyl isothiocyanate	291:310	allyl isothiocyanate (AIT)	291:316	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	15	theme	barley	319:324	arg1	solvent					478:484	solvent	478:484	solvent	478:484	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	4	16	theme	Listeria	691:698	arg1	innocua					700:706	Listeria innocua	691:706	Listeria innocua	691:706	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	17	from	%	1089:1089	arg1	addition					1095:1102	addition	1095:1102	addition	1095:1102	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	17	from	%	1089:1089	arg1	chitosan					1066:1073	chitosan	1066:1073	chitosan from 1.5% to 3%	1066:1089	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	17	from	%	1089:1089	arg1	increase					1054:1061	the increase	1050:1061	the increase of chitosan from 1.5% to 3%	1050:1089	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	18	from	4logCFU/ml	829:838	arg1	TSB					843:845	TSB	843:845	TSB	843:845	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	3	19	theme	homogenization	551:564	arg1	processing					572:581	high pressure homogenization (HPH) processing	537:581	high pressure homogenization (HPH) processing	537:581	Micro-emulsions were obtained using high pressure homogenization (HPH) processing at 138MPa for 3cycles.
26057110	4	20	theme	HPH	984:986	arg1	processing					988:997	HPH processing	984:997	HPH processing	984:997	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	1	21	theme	Edible	101:106	arg1	films					132:136	Edible antimicrobial composite films	101:136	Edible antimicrobial composite films from micro-emulsions containing all natural compounds	101:190	Edible antimicrobial composite films from micro-emulsions containing all natural compounds were developed and their antimicrobial properties and microstructures were investigated.
26057110	4	22	theme	3	1088:1088	arg1	%					1083:1083	%	1083:1083	%	1083:1083	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	1	23	theme	antimicrobial	217:229	arg1	properties					231:240	their antimicrobial properties	211:240	their antimicrobial properties	211:240	Edible antimicrobial composite films from micro-emulsions containing all natural compounds were developed and their antimicrobial properties and microstructures were investigated.
26057110	0	24	theme	Antimicrobial	0:12	arg1	property					14:21	Antimicrobial property	0:21	Antimicrobial property	0:21	Antimicrobial property and microstructure of micro-emulsion edible composite films against Listeria.
26057110	4	25	theme	films	974:978	arg1	property					958:965	the antimicrobial property	940:965	the antimicrobial property of the films	940:978	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	1	26	theme	antimicrobial	108:120	arg1	films					132:136	Edible antimicrobial composite films	101:136	Edible antimicrobial composite films from micro-emulsions containing all natural compounds	101:190	Edible antimicrobial composite films from micro-emulsions containing all natural compounds were developed and their antimicrobial properties and microstructures were investigated.
26057110	4	27	theme	chitosan	1066:1073	arg1	addition					1095:1102	addition	1095:1102	addition	1095:1102	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	27	theme	chitosan	1066:1073	arg1	increase					1054:1061	the increase	1050:1061	the increase of chitosan from 1.5% to 3%	1050:1089	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	2	28	theme	levulinic	391:399	arg1	acids					365:369	organic acids	357:369	organic acids (acetic, lactic and levulinic acids)	357:406	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	28	theme	levulinic	391:399	arg1	acids					401:405	acetic, lactic and levulinic acids	372:405	acetic, lactic and levulinic acids	372:405	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	29	theme	antimicrobial	441:453	arg1	agent					455:459	antimicrobial agent	441:459	antimicrobial agent	441:459	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	29	theme	antimicrobial	441:453	arg1	acids					365:369	organic acids	357:369	organic acids (acetic, lactic and levulinic acids)	357:406	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	29	theme	antimicrobial	441:453	arg1	arabinoxylan					332:343	barley straw arabinoxylan	319:343	barley straw arabinoxylan (BSAX)	319:350	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	29	theme	antimicrobial	441:453	arg1	Chitosan					281:288	Chitosan	281:288	Chitosan	281:288	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	29	theme	antimicrobial	441:453	arg1	isothiocyanate					297:310	allyl isothiocyanate	291:310	allyl isothiocyanate (AIT)	291:316	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	1	30	from	micro-emulsions	143:157	arg1	films					132:136	Edible antimicrobial composite films	101:136	Edible antimicrobial composite films from micro-emulsions containing all natural compounds	101:190	Edible antimicrobial composite films from micro-emulsions containing all natural compounds were developed and their antimicrobial properties and microstructures were investigated.
26057110	1	31	theme	composite	122:130	arg1	films					132:136	Edible antimicrobial composite films	101:136	Edible antimicrobial composite films from micro-emulsions containing all natural compounds	101:190	Edible antimicrobial composite films from micro-emulsions containing all natural compounds were developed and their antimicrobial properties and microstructures were investigated.
26057110	4	32	theme	microbial	800:808	arg1	reductions					810:819	microbial reductions	800:819	microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects	800:1187	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	3	33	theme	pressure	542:549	arg1	HPH					567:569	HPH	567:569	HPH	567:569	Micro-emulsions were obtained using high pressure homogenization (HPH) processing at 138MPa for 3cycles.
26057110	3	33	theme	pressure	542:549	arg1	homogenization					551:564	high pressure homogenization	537:564	high pressure homogenization (HPH) processing	537:581	Micro-emulsions were obtained using high pressure homogenization (HPH) processing at 138MPa for 3cycles.
26057110	4	34	theme	tryptic	711:717	arg1	TSB					730:732	TSB	730:732	TSB	730:732	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	34	theme	tryptic	711:717	arg1	broth					723:727	tryptic soy broth	711:727	tryptic soy broth (TSB)	711:733	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	35	theme	1.5	1080:1082	arg1	%					1083:1083	%	1083:1083	%	1083:1083	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	2	36	theme	film-forming	421:432	arg1	acids					365:369	organic acids	357:369	organic acids (acetic, lactic and levulinic acids)	357:406	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	36	theme	film-forming	421:432	arg1	arabinoxylan					332:343	barley straw arabinoxylan	319:343	barley straw arabinoxylan (BSAX)	319:350	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	36	theme	film-forming	421:432	arg1	Chitosan					281:288	Chitosan	281:288	Chitosan	281:288	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	36	theme	film-forming	421:432	arg1	isothiocyanate					297:310	allyl isothiocyanate	291:310	allyl isothiocyanate (AIT)	291:316	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	36	theme	film-forming	421:432	arg1	agent					434:438	film-forming agent	421:438	film-forming agent	421:438	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	0	37	theme	micro-emulsion	45:58	arg1	films					77:81	micro-emulsion edible composite films	45:81	micro-emulsion edible composite films	45:81	Antimicrobial property and microstructure of micro-emulsion edible composite films against Listeria.
26057110	4	38	theme	10°C.	903:907	arg1	AIT					909:911	10°C. AIT	903:911	10°C. AIT	903:911	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	2	39	theme	allyl	291:295	arg1	Chitosan					281:288	Chitosan	281:288	Chitosan	281:288	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	39	theme	allyl	291:295	arg1	isothiocyanate					297:310	allyl isothiocyanate	291:310	allyl isothiocyanate (AIT)	291:316	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	39	theme	allyl	291:295	arg1	agent					455:459	antimicrobial agent	441:459	antimicrobial agent	441:459	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	39	theme	allyl	291:295	arg1	arabinoxylan					332:343	barley straw arabinoxylan	319:343	barley straw arabinoxylan (BSAX)	319:350	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	39	theme	allyl	291:295	arg1	emulsifier					462:471	emulsifier	462:471	emulsifier	462:471	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	39	theme	allyl	291:295	arg1	agent					434:438	film-forming agent	421:438	film-forming agent	421:438	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	39	theme	allyl	291:295	arg1	acids					365:369	organic acids	357:369	organic acids (acetic, lactic and levulinic acids)	357:406	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	39	theme	allyl	291:295	arg1	AIT					313:315	AIT	313:315	AIT	313:315	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	39	theme	allyl	291:295	arg1	solvent					478:484	solvent	478:484	solvent	478:484	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	5	40	theme	antimicrobial	1261:1273	arg1	films					1275:1279	new edible antimicrobial films	1250:1279	new edible antimicrobial films	1250:1279	This study demonstrated an effective approach to developing new edible antimicrobial films and coatings used for food applications.
26057110	4	41	dep	significantly	656:668	arg1	p<0.05					671:676	p<0.05	671:676	p<0.05	671:676	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	2	42	used	used	413:416	arg2	agent					434:438	film-forming agent	421:438	film-forming agent	421:438	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	42	used	used	413:416	arg2	AIT					313:315	AIT	313:315	AIT	313:315	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	42	used	used	413:416	arg2	arabinoxylan					332:343	barley straw arabinoxylan	319:343	barley straw arabinoxylan (BSAX)	319:350	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	42	used	used	413:416	arg2	emulsifier					462:471	emulsifier	462:471	emulsifier	462:471	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	42	used	used	413:416	arg2	solvent					478:484	solvent	478:484	solvent	478:484	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	42	used	used	413:416	arg2	BSAX					346:349	BSAX	346:349	BSAX	346:349	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	42	used	used	413:416	arg2	isothiocyanate					297:310	allyl isothiocyanate	291:310	allyl isothiocyanate (AIT)	291:316	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	42	used	used	413:416	arg2	acids					401:405	acetic, lactic and levulinic acids	372:405	acetic, lactic and levulinic acids	372:405	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	42	used	used	413:416	arg2	acids					365:369	organic acids	357:369	organic acids (acetic, lactic and levulinic acids)	357:406	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	42	used	used	413:416	arg2	Chitosan					281:288	Chitosan	281:288	Chitosan	281:288	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	42	used	used	413:416	arg2	agent					455:459	antimicrobial agent	441:459	antimicrobial agent	441:459	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	0	43	theme	composite	67:75	arg1	films					77:81	micro-emulsion edible composite films	45:81	micro-emulsion edible composite films	45:81	Antimicrobial property and microstructure of micro-emulsion edible composite films against Listeria.
26057110	4	44	theme	acetic	1107:1112	arg1	acid					1114:1117	acetic acid	1107:1117	acetic acid to the formulations	1107:1137	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	45	from	increase	1054:1061	arg1	%					1089:1089	1.5% to 3%	1080:1089	1.5% to 3%	1080:1089	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	0	46	theme	edible	60:65	arg1	films					77:81	micro-emulsion edible composite films	45:81	micro-emulsion edible composite films	45:81	Antimicrobial property and microstructure of micro-emulsion edible composite films against Listeria.
26057110	5	47	theme	new	1250:1252	arg1	films					1275:1279	new edible antimicrobial films	1250:1279	new edible antimicrobial films	1250:1279	This study demonstrated an effective approach to developing new edible antimicrobial films and coatings used for food applications.
26057110	4	48	theme	meat	776:779	arg1	samples					781:787	ready-to-eat (RTE) meat samples	757:787	ready-to-eat (RTE) meat samples	757:787	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	2	49	theme	organic	357:363	arg1	Chitosan					281:288	Chitosan	281:288	Chitosan	281:288	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	49	theme	organic	357:363	arg1	agent					455:459	antimicrobial agent	441:459	antimicrobial agent	441:459	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	49	theme	organic	357:363	arg1	arabinoxylan					332:343	barley straw arabinoxylan	319:343	barley straw arabinoxylan (BSAX)	319:350	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	49	theme	organic	357:363	arg1	emulsifier					462:471	emulsifier	462:471	emulsifier	462:471	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	49	theme	organic	357:363	arg1	agent					434:438	film-forming agent	421:438	film-forming agent	421:438	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	49	theme	organic	357:363	arg1	acids					365:369	organic acids	357:369	organic acids (acetic, lactic and levulinic acids)	357:406	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	49	theme	organic	357:363	arg1	isothiocyanate					297:310	allyl isothiocyanate	291:310	allyl isothiocyanate (AIT)	291:316	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	49	theme	organic	357:363	arg1	acids					401:405	acetic, lactic and levulinic acids	372:405	acetic, lactic and levulinic acids	372:405	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	49	theme	organic	357:363	arg1	solvent					478:484	solvent	478:484	solvent	478:484	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	5	50	theme	effective	1217:1225	arg1	approach					1227:1234	an effective approach	1214:1234	an effective approach to developing new edible antimicrobial films and coatings used for food applications	1214:1319	This study demonstrated an effective approach to developing new edible antimicrobial films and coatings used for food applications.
26057110	4	51	theme	RTE	771:773	arg1	samples					781:787	ready-to-eat (RTE) meat samples	757:787	ready-to-eat (RTE) meat samples	757:787	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	52	theme	acid	1114:1117	arg1	addition					1095:1102	addition	1095:1102	addition	1095:1102	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	52	theme	acid	1114:1117	arg1	increase					1054:1061	the increase	1050:1061	the increase of chitosan from 1.5% to 3%	1050:1089	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	3	53	theme	high	537:540	arg1	HPH					567:569	HPH	567:569	HPH	567:569	Micro-emulsions were obtained using high pressure homogenization (HPH) processing at 138MPa for 3cycles.
26057110	3	53	theme	high	537:540	arg1	homogenization					551:564	high pressure homogenization	537:564	high pressure homogenization (HPH) processing	537:581	Micro-emulsions were obtained using high pressure homogenization (HPH) processing at 138MPa for 3cycles.
26057110	4	54	theme	composite	610:618	arg1	films					620:624	The composite films	606:624	The composite films made from the micro-emulsions	606:654	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	5	55	theme	food	1303:1306	arg1	applications					1308:1319	food applications	1303:1319	food applications	1303:1319	This study demonstrated an effective approach to developing new edible antimicrobial films and coatings used for food applications.
26057110	1	56	theme	natural	174:180	arg1	compounds					182:190	all natural compounds	170:190	all natural compounds	170:190	Edible antimicrobial composite films from micro-emulsions containing all natural compounds were developed and their antimicrobial properties and microstructures were investigated.
26057110	0	57	theme	films	77:81	arg1	microstructure					27:40	microstructure	27:40	microstructure	27:40	Antimicrobial property and microstructure of micro-emulsion edible composite films against Listeria.
26057110	0	57	theme	films	77:81	arg1	property					14:21	Antimicrobial property	0:21	Antimicrobial property	0:21	Antimicrobial property and microstructure of micro-emulsion edible composite films against Listeria.
26057110	4	58	from	22°C	862:865	arg1	2days					853:857	2days	853:857	2days at 22°C and on meat samples after 35days at 10°C. AIT	853:911	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	4	59	theme	samples	781:787	arg1	surface					746:752	the surface	742:752	the surface of ready-to-eat (RTE) meat samples	742:787	The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects.
26057110	5	60	theme	edible	1254:1259	arg1	films					1275:1279	new edible antimicrobial films	1250:1279	new edible antimicrobial films	1250:1279	This study demonstrated an effective approach to developing new edible antimicrobial films and coatings used for food applications.
26057110	2	61	theme	lactic	380:385	arg1	acids					365:369	organic acids	357:369	organic acids (acetic, lactic and levulinic acids)	357:406	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
26057110	2	61	theme	lactic	380:385	arg1	acids					401:405	acetic, lactic and levulinic acids	372:405	acetic, lactic and levulinic acids	372:405	Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively.
29122713	2	0	theme	scanning	498:505	arg1	SEM					528:530	SEM	528:530	SEM	528:530	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	2	0	theme	scanning	498:505	arg1	microscopy					516:525	scanning electron microscopy	498:525	scanning electron microscopy (SEM)	498:531	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	1	1	theme	weight	352:357	arg1	ratios					359:364	different inorganic/organic weight ratios	324:364	different inorganic/organic weight ratios	324:364	In this investigation, porous composite scaffolds were prepared using a freeze-drying procedure by mixing zein (ZN), chitosan (CS) and nanohydroxyapatite (nHAp) in different inorganic/organic weight ratios.
29122713	1	2	theme	composite	190:198	arg1	scaffolds					200:208	porous composite scaffolds	183:208	porous composite scaffolds	183:208	In this investigation, porous composite scaffolds were prepared using a freeze-drying procedure by mixing zein (ZN), chitosan (CS) and nanohydroxyapatite (nHAp) in different inorganic/organic weight ratios.
29122713	3	3	theme	cell	700:703	arg1	attachment					705:714	cell attachment	700:714	cell attachment	700:714	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	6	4	theme	non-	1139:1142	arg1	toxicity					1144:1151	non- toxicity	1139:1151	non- toxicity	1139:1151	Studies indicated non- toxicity to the cells, and the cells were found to be attached to the pore walls within the scaffolds.
29122713	4	5	with	nature	805:810	arg1	interconnections					848:863	interconnections	848:863	interconnections for cell penetration and colonization	848:901	The results showed a porous nature with acceptable pore dimensions and interconnections for cell penetration and colonization.
29122713	4	5	with	nature	805:810	arg1	dimensions					833:842	acceptable pore dimensions	817:842	acceptable pore dimensions	817:842	The results showed a porous nature with acceptable pore dimensions and interconnections for cell penetration and colonization.
29122713	0	6	theme	bone	135:138	arg1	engineering					147:157	bone tissue engineering	135:157	bone tissue engineering	135:157	Fabrication and characterization of nanobiocomposite scaffold of zein/chitosan/nanohydroxyapatite prepared by freeze-drying method for bone tissue engineering.
29122713	6	7	theme	pore	1214:1217	arg1	walls					1219:1223	the pore walls	1210:1223	the pore walls within the scaffolds	1210:1244	Studies indicated non- toxicity to the cells, and the cells were found to be attached to the pore walls within the scaffolds.
29122713	5	8	theme	4,5-dimethylthiazol-2-yl	986:1009	arg1	MTT					1046:1048	MTT	1046:1048	MTT	1046:1048	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	5	8	theme	4,5-dimethylthiazol-2-yl	986:1009	arg1	bromide					1037:1043	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide	983:1043	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation	983:1060	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	2	9	theme	gained	371:376	arg1	scaffolds					392:400	The gained nanocomposite scaffolds	367:400	The gained nanocomposite scaffolds	367:400	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	5	10	theme	scaffolds	957:965	arg1	cytocompatibility					921:937	the cytocompatibility	917:937	the cytocompatibility of the ZN/CS/nHAp scaffolds	917:965	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	2	11	dep	Fourier	449:455	arg1	transform					457:465	transform	457:465	transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique	457:605	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	2	12	theme	X-ray	421:425	arg1	XRD					443:445	XRD	443:445	XRD	443:445	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	2	12	theme	X-ray	421:425	arg1	Fourier					449:455	Fourier	449:455	Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique	449:605	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	2	12	theme	X-ray	421:425	arg1	diffractometer					427:440	X-ray diffractometer	421:440	X-ray diffractometer (XRD)	421:446	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	0	13	theme	tissue	140:145	arg1	engineering					147:157	bone tissue engineering	135:157	bone tissue engineering	135:157	Fabrication and characterization of nanobiocomposite scaffold of zein/chitosan/nanohydroxyapatite prepared by freeze-drying method for bone tissue engineering.
29122713	3	14	theme	cell	721:724	arg1	viability					726:734	cell viability	721:734	cell viability	721:734	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	5	15	theme	-2,5-diphenyl	1011:1023	arg1	MTT					1046:1048	MTT	1046:1048	MTT	1046:1048	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	5	15	theme	-2,5-diphenyl	1011:1023	arg1	bromide					1037:1043	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide	983:1043	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation	983:1060	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	6	16	attach	attached	1198:1205	arg1	walls					1219:1223	the pore walls	1210:1223	the pore walls within the scaffolds	1210:1244	Studies indicated non- toxicity to the cells, and the cells were found to be attached to the pore walls within the scaffolds.
29122713	6	16	attach	attached	1198:1205	arg2	cells					1175:1179	the cells	1171:1179	the cells	1171:1179	Studies indicated non- toxicity to the cells, and the cells were found to be attached to the pore walls within the scaffolds.
29122713	7	17	theme	potential	1372:1380	arg1	materials					1392:1400	potential candidate materials	1372:1400	potential candidate materials for the tissue engineering	1372:1427	The results related to physicochemical properties and superior cytocompatibility suggested that ZN/CS/nHAp scaffold could be potential candidate materials for the tissue engineering.
29122713	4	18	dep	cell	869:872	arg1	penetration					874:884	penetration	874:884	penetration	874:884	The results showed a porous nature with acceptable pore dimensions and interconnections for cell penetration and colonization.
29122713	2	19	dep	transform	457:465	arg1	infrared					467:474	infrared	467:474	transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique	457:605	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	5	20	theme	cancer	1107:1112	arg1	cells					1114:1118	human bone cancer cells	1096:1118	human bone cancer cells	1096:1118	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	1	21	theme	freeze-drying	232:244	arg1	procedure					246:254	a freeze-drying procedure	230:254	a freeze-drying procedure	230:254	In this investigation, porous composite scaffolds were prepared using a freeze-drying procedure by mixing zein (ZN), chitosan (CS) and nanohydroxyapatite (nHAp) in different inorganic/organic weight ratios.
29122713	3	22	theme	composite	743:751	arg1	scaffolds					753:761	the composite scaffolds	739:761	the composite scaffolds	739:761	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	5	23	theme	bone	1102:1105	arg1	cells					1114:1118	human bone cancer cells	1096:1118	human bone cancer cells	1096:1118	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	7	24	theme	ZN/CS/nHAp	1343:1352	arg1	scaffold					1354:1361	ZN/CS/nHAp scaffold	1343:1361	ZN/CS/nHAp scaffold	1343:1361	The results related to physicochemical properties and superior cytocompatibility suggested that ZN/CS/nHAp scaffold could be potential candidate materials for the tissue engineering.
29122713	0	25	theme	scaffold	53:60	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of nanobiocomposite scaffold of zein/chitosan/nanohydroxyapatite prepared by freeze-drying method for bone tissue engineering.
29122713	0	25	theme	scaffold	53:60	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of nanobiocomposite scaffold of zein/chitosan/nanohydroxyapatite prepared by freeze-drying method for bone tissue engineering.
29122713	3	26	theme	scaffolds	753:761	arg1	viability					726:734	cell viability	721:734	cell viability	721:734	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	3	26	theme	scaffolds	753:761	arg1	properties					645:654	mechanical properties	634:654	mechanical properties	634:654	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	3	26	theme	scaffolds	753:761	arg1	attachment					705:714	cell attachment	700:714	cell attachment	700:714	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	3	26	theme	scaffolds	753:761	arg1	swelling					614:621	swelling	614:621	swelling	614:621	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	3	26	theme	scaffolds	753:761	arg1	porosity					624:631	porosity	624:631	porosity	624:631	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	3	26	theme	scaffolds	753:761	arg1	capability					675:684	biomineralization capability	657:684	biomineralization capability	657:684	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	3	26	theme	scaffolds	753:761	arg1	degradation					687:697	degradation	687:697	degradation	687:697	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	3	27	theme	biomineralization	657:673	arg1	capability					675:684	biomineralization capability	657:684	biomineralization capability	657:684	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	4	28	theme	pore	828:831	arg1	dimensions					833:842	acceptable pore dimensions	817:842	acceptable pore dimensions	817:842	The results showed a porous nature with acceptable pore dimensions and interconnections for cell penetration and colonization.
29122713	0	29	theme	nanobiocomposite	36:51	arg1	scaffold					53:60	nanobiocomposite scaffold	36:60	nanobiocomposite scaffold	36:60	Fabrication and characterization of nanobiocomposite scaffold of zein/chitosan/nanohydroxyapatite prepared by freeze-drying method for bone tissue engineering.
29122713	2	30	theme	thermogravimetric	534:550	arg1	TGA					562:564	TGA	562:564	TGA	562:564	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	2	30	theme	thermogravimetric	534:550	arg1	analysis					552:559	thermogravimetric analysis	534:559	thermogravimetric analysis (TGA)	534:565	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	3	31	theme	mechanical	634:643	arg1	properties					645:654	mechanical properties	634:654	mechanical properties	634:654	Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied.
29122713	4	32	theme	acceptable	817:826	arg1	dimensions					833:842	acceptable pore dimensions	817:842	acceptable pore dimensions	817:842	The results showed a porous nature with acceptable pore dimensions and interconnections for cell penetration and colonization.
29122713	0	33	theme	zein/chitosan/nanohydroxyapatite	65:96	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of nanobiocomposite scaffold of zein/chitosan/nanohydroxyapatite prepared by freeze-drying method for bone tissue engineering.
29122713	0	33	theme	zein/chitosan/nanohydroxyapatite	65:96	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of nanobiocomposite scaffold of zein/chitosan/nanohydroxyapatite prepared by freeze-drying method for bone tissue engineering.
29122713	2	34	theme	N2-adsorption-desorption	572:595	arg1	technique					597:605	N2-adsorption-desorption technique	572:605	N2-adsorption-desorption technique	572:605	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	7	35	theme	tissue	1410:1415	arg1	engineering					1417:1427	the tissue engineering	1406:1427	the tissue engineering	1406:1427	The results related to physicochemical properties and superior cytocompatibility suggested that ZN/CS/nHAp scaffold could be potential candidate materials for the tissue engineering.
29122713	5	36	theme	attachment	1071:1080	arg1	studies					1082:1088	cell attachment studies	1066:1088	cell attachment studies using human bone cancer cells	1066:1118	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	5	37	theme	cell	1066:1069	arg1	studies					1082:1088	cell attachment studies	1066:1088	cell attachment studies using human bone cancer cells	1066:1118	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	7	38	theme	superior	1301:1308	arg1	cytocompatibility					1310:1326	superior cytocompatibility	1301:1326	superior cytocompatibility	1301:1326	The results related to physicochemical properties and superior cytocompatibility suggested that ZN/CS/nHAp scaffold could be potential candidate materials for the tissue engineering.
29122713	1	39	theme	different	324:332	arg1	ratios					359:364	different inorganic/organic weight ratios	324:364	different inorganic/organic weight ratios	324:364	In this investigation, porous composite scaffolds were prepared using a freeze-drying procedure by mixing zein (ZN), chitosan (CS) and nanohydroxyapatite (nHAp) in different inorganic/organic weight ratios.
29122713	5	40	theme	tetrazolium	1025:1035	arg1	MTT					1046:1048	MTT	1046:1048	MTT	1046:1048	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	5	40	theme	tetrazolium	1025:1035	arg1	bromide					1037:1043	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide	983:1043	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation	983:1060	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	4	41	theme	porous	798:803	arg1	nature					805:810	a porous nature	796:810	a porous nature with acceptable pore dimensions and interconnections for cell penetration and colonization	796:901	The results showed a porous nature with acceptable pore dimensions and interconnections for cell penetration and colonization.
29122713	5	42	theme	ZN/CS/nHAp	946:955	arg1	scaffolds					957:965	the ZN/CS/nHAp scaffolds	942:965	the ZN/CS/nHAp scaffolds	942:965	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	5	43	theme	bromide	1037:1043	arg1	evaluation					1051:1060	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation	983:1060	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation	983:1060	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	1	44	theme	porous	183:188	arg1	scaffolds					200:208	porous composite scaffolds	183:208	porous composite scaffolds	183:208	In this investigation, porous composite scaffolds were prepared using a freeze-drying procedure by mixing zein (ZN), chitosan (CS) and nanohydroxyapatite (nHAp) in different inorganic/organic weight ratios.
29122713	7	45	theme	physicochemical	1270:1284	arg1	properties					1286:1295	physicochemical properties	1270:1295	physicochemical properties	1270:1295	The results related to physicochemical properties and superior cytocompatibility suggested that ZN/CS/nHAp scaffold could be potential candidate materials for the tissue engineering.
29122713	5	46	theme	human	1096:1100	arg1	cells					1114:1118	human bone cancer cells	1096:1118	human bone cancer cells	1096:1118	In addition, the cytocompatibility of the ZN/CS/nHAp scaffolds was surveyed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) evaluation and cell attachment studies using human bone cancer cells.
29122713	7	47	theme	candidate	1382:1390	arg1	materials					1392:1400	potential candidate materials	1372:1400	potential candidate materials for the tissue engineering	1372:1427	The results related to physicochemical properties and superior cytocompatibility suggested that ZN/CS/nHAp scaffold could be potential candidate materials for the tissue engineering.
29122713	7	48	theme	related	1259:1265	arg1	results					1251:1257	The results	1247:1257	The results related to physicochemical properties and superior cytocompatibility	1247:1326	The results related to physicochemical properties and superior cytocompatibility suggested that ZN/CS/nHAp scaffold could be potential candidate materials for the tissue engineering.
29122713	2	49	theme	electron	507:514	arg1	SEM					528:530	SEM	528:530	SEM	528:530	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	2	49	theme	electron	507:514	arg1	microscopy					516:525	scanning electron microscopy	498:525	scanning electron microscopy (SEM)	498:531	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
29122713	1	50	theme	inorganic/organic	334:350	arg1	ratios					359:364	different inorganic/organic weight ratios	324:364	different inorganic/organic weight ratios	324:364	In this investigation, porous composite scaffolds were prepared using a freeze-drying procedure by mixing zein (ZN), chitosan (CS) and nanohydroxyapatite (nHAp) in different inorganic/organic weight ratios.
29122713	2	51	theme	nanocomposite	378:390	arg1	scaffolds					392:400	The gained nanocomposite scaffolds	367:400	The gained nanocomposite scaffolds	367:400	The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique.
26993532	2	0	theme	Electron	454:461	arg1	Microscopy					463:472	Transmission Electron Microscopy	441:472	Transmission Electron Microscopy (TEM)	441:478	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	2	0	theme	Electron	454:461	arg1	TEM					475:477	TEM	475:477	TEM	475:477	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	4	1	theme	highest	768:774	arg1	86.17mg/g					797:805	86.17mg/g	797:805	86.17mg/g	797:805	The results indicate that the adsorption of Cr(VI) on GEC was highly pH-dependent, with the highest adsorption capacity (86.17mg/g) occurring at pH 2.
26993532	4	1	theme	highest	768:774	arg1	capacity					787:794	the highest adsorption capacity	764:794	the highest adsorption capacity (86.17mg/g)	764:806	The results indicate that the adsorption of Cr(VI) on GEC was highly pH-dependent, with the highest adsorption capacity (86.17mg/g) occurring at pH 2.
26993532	9	2	theme	practical	1329:1337	arg1	application					1339:1349	practical application	1329:1349	practical application	1329:1349	The further regeneration experiments showed the adsorption capacity of GEC for Cr(VI) decreased 5% after 7 times reuse, indicating the potential of the as-prepared material for practical application.
26993532	2	3	theme	Transmission	441:452	arg1	Microscopy					463:472	Transmission Electron Microscopy	441:472	Transmission Electron Microscopy (TEM)	441:478	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	2	3	theme	Transmission	441:452	arg1	TEM					475:477	TEM	475:477	TEM	475:477	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	3	4	theme	Cr	634:635	arg1	adsorption					620:629	the adsorption	616:629	the adsorption of Cr(VI) by GEC	616:646	Batch adsorption experiments were carried out to evaluate the adsorption of Cr(VI) by GEC under different conditions.
26993532	2	5	theme	X-ray	481:485	arg1	XRD					500:502	XRD	500:502	XRD	500:502	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	2	5	theme	X-ray	481:485	arg1	diffraction					487:497	X-ray diffraction	481:497	X-ray diffraction (XRD)	481:503	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	6	6	theme	adsorption	898:907	arg1	data					909:912	The adsorption data	894:912	The adsorption data	894:912	The adsorption data were well described by the Freundlich isotherm model.
26993532	4	7	theme	adsorption	776:785	arg1	86.17mg/g					797:805	86.17mg/g	797:805	86.17mg/g	797:805	The results indicate that the adsorption of Cr(VI) on GEC was highly pH-dependent, with the highest adsorption capacity (86.17mg/g) occurring at pH 2.
26993532	4	7	theme	adsorption	776:785	arg1	capacity					787:794	the highest adsorption capacity	764:794	the highest adsorption capacity (86.17mg/g)	764:806	The results indicate that the adsorption of Cr(VI) on GEC was highly pH-dependent, with the highest adsorption capacity (86.17mg/g) occurring at pH 2.
26993532	5	8	theme	pseudo-second-order	864:882	arg1	behavior					884:891	pseudo-second-order behavior	864:891	pseudo-second-order behavior	864:891	The kinetics of adsorption exhibited pseudo-second-order behavior.
26993532	9	9	dep	decreased	1238:1246	arg1	VI					1234:1235	VI	1234:1235	VI	1234:1235	The further regeneration experiments showed the adsorption capacity of GEC for Cr(VI) decreased 5% after 7 times reuse, indicating the potential of the as-prepared material for practical application.
26993532	3	10	theme	different	654:662	arg1	conditions					664:673	different conditions	654:673	different conditions	654:673	Batch adsorption experiments were carried out to evaluate the adsorption of Cr(VI) by GEC under different conditions.
26993532	8	11	theme	feasible	1134:1141	arg1	adsorption					1089:1098	the adsorption	1085:1098	the adsorption	1085:1098	The calculated thermodynamic parameters indicate that the adsorption is a spontaneous, endothermic and feasible process.
26993532	8	11	theme	feasible	1134:1141	arg1	process					1143:1149	a spontaneous, endothermic and feasible process	1103:1149	a spontaneous, endothermic and feasible process	1103:1149	The calculated thermodynamic parameters indicate that the adsorption is a spontaneous, endothermic and feasible process.
26993532	8	12	theme	spontaneous	1105:1115	arg1	adsorption					1089:1098	the adsorption	1085:1098	the adsorption	1085:1098	The calculated thermodynamic parameters indicate that the adsorption is a spontaneous, endothermic and feasible process.
26993532	8	12	theme	spontaneous	1105:1115	arg1	process					1143:1149	a spontaneous, endothermic and feasible process	1103:1149	a spontaneous, endothermic and feasible process	1103:1149	The calculated thermodynamic parameters indicate that the adsorption is a spontaneous, endothermic and feasible process.
26993532	8	13	theme	endothermic	1118:1128	arg1	adsorption					1089:1098	the adsorption	1085:1098	the adsorption	1085:1098	The calculated thermodynamic parameters indicate that the adsorption is a spontaneous, endothermic and feasible process.
26993532	8	13	theme	endothermic	1118:1128	arg1	process					1143:1149	a spontaneous, endothermic and feasible process	1103:1149	a spontaneous, endothermic and feasible process	1103:1149	The calculated thermodynamic parameters indicate that the adsorption is a spontaneous, endothermic and feasible process.
26993532	1	14	theme	hexavalent	138:147	arg1	chromium					149:156	hexavalent chromium	138:156	hexavalent chromium (Cr(VI))	138:165	A novel adsorbent for removal of hexavalent chromium (Cr(VI)) from aqueous solutions has been successfully prepared by modifying graphene oxide/chitosan composite with disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC).
26993532	1	14	theme	hexavalent	138:147	arg1	Cr					159:160	Cr(VI)	159:164	Cr(VI)	159:164	A novel adsorbent for removal of hexavalent chromium (Cr(VI)) from aqueous solutions has been successfully prepared by modifying graphene oxide/chitosan composite with disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC).
26993532	0	15	theme	novel	2:6	arg1	graphene					17:24	A novel modified graphene	0:24	A novel modified graphene	0:24	A novel modified graphene oxide/chitosan composite used as an adsorbent for Cr(VI) in aqueous solutions.
26993532	2	16	dep	transform	517:525	arg1	infrared					527:534	infrared	527:534	transform infrared spectroscopy (FT-IR)	517:555	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	0	17	dep	Cr	76:77	arg1	VI					79:80	VI	79:80	VI	79:80	A novel modified graphene oxide/chitosan composite used as an adsorbent for Cr(VI) in aqueous solutions.
26993532	1	18	theme	chromium	149:156	arg1	removal					127:133	removal	127:133	removal of hexavalent chromium (Cr(VI)) from aqueous solutions	127:188	A novel adsorbent for removal of hexavalent chromium (Cr(VI)) from aqueous solutions has been successfully prepared by modifying graphene oxide/chitosan composite with disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC).
26993532	2	19	theme	electron	414:421	arg1	SEM					435:437	SEM	435:437	SEM	435:437	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	2	19	theme	electron	414:421	arg1	microscopy					423:432	scanning electron microscopy	405:432	scanning electron microscopy (SEM)	405:438	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	3	20	dep	Cr	634:635	arg1	VI					637:638	VI	637:638	VI	637:638	Batch adsorption experiments were carried out to evaluate the adsorption of Cr(VI) by GEC under different conditions.
26993532	9	21	theme	further	1156:1162	arg1	experiments					1177:1187	The further regeneration experiments	1152:1187	The further regeneration experiments	1152:1187	The further regeneration experiments showed the adsorption capacity of GEC for Cr(VI) decreased 5% after 7 times reuse, indicating the potential of the as-prepared material for practical application.
26993532	2	22	theme	modified	333:340	arg1	composite					342:350	This modified composite	328:350	This modified composite	328:350	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	2	23	theme	scanning	405:412	arg1	SEM					435:437	SEM	435:437	SEM	435:437	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	2	23	theme	scanning	405:412	arg1	microscopy					423:432	scanning electron microscopy	405:432	scanning electron microscopy (SEM)	405:438	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	9	24	theme	times	1259:1263	arg1	reuse					1265:1269	7 times reuse	1257:1269	7 times reuse	1257:1269	The further regeneration experiments showed the adsorption capacity of GEC for Cr(VI) decreased 5% after 7 times reuse, indicating the potential of the as-prepared material for practical application.
26993532	9	25	theme	regeneration	1164:1175	arg1	experiments					1177:1187	The further regeneration experiments	1152:1187	The further regeneration experiments	1152:1187	The further regeneration experiments showed the adsorption capacity of GEC for Cr(VI) decreased 5% after 7 times reuse, indicating the potential of the as-prepared material for practical application.
26993532	0	26	theme	modified	8:15	arg1	graphene					17:24	A novel modified graphene	0:24	A novel modified graphene	0:24	A novel modified graphene oxide/chitosan composite used as an adsorbent for Cr(VI) in aqueous solutions.
26993532	1	27	theme	graphene	234:241	arg1	composite					258:266	graphene oxide/chitosan composite	234:266	graphene oxide/chitosan composite	234:266	A novel adsorbent for removal of hexavalent chromium (Cr(VI)) from aqueous solutions has been successfully prepared by modifying graphene oxide/chitosan composite with disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC).
26993532	0	28	theme	aqueous	86:92	arg1	solutions					94:102	aqueous solutions	86:102	aqueous solutions	86:102	A novel modified graphene oxide/chitosan composite used as an adsorbent for Cr(VI) in aqueous solutions.
26993532	0	29	from	Cr	76:77	arg1	solutions					94:102	aqueous solutions	86:102	aqueous solutions	86:102	A novel modified graphene oxide/chitosan composite used as an adsorbent for Cr(VI) in aqueous solutions.
26993532	5	30	theme	adsorption	843:852	arg1	kinetics					831:838	The kinetics	827:838	The kinetics of adsorption	827:852	The kinetics of adsorption exhibited pseudo-second-order behavior.
26993532	1	31	theme	oxide/chitosan	243:256	arg1	composite					258:266	graphene oxide/chitosan composite	234:266	graphene oxide/chitosan composite	234:266	A novel adsorbent for removal of hexavalent chromium (Cr(VI)) from aqueous solutions has been successfully prepared by modifying graphene oxide/chitosan composite with disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC).
26993532	6	32	theme	isotherm	952:959	arg1	model					961:965	the Freundlich isotherm model	937:965	the Freundlich isotherm model	937:965	The adsorption data were well described by the Freundlich isotherm model.
26993532	2	33	theme	various	373:379	arg1	Microscopy					463:472	Transmission Electron Microscopy	441:472	Transmission Electron Microscopy (TEM)	441:478	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	2	33	theme	various	373:379	arg1	Fourier					509:515	Fourier	509:515	Fourier transform infrared spectroscopy (FT-IR)	509:555	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	2	33	theme	various	373:379	arg1	diffraction					487:497	X-ray diffraction	481:497	X-ray diffraction (XRD)	481:503	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	2	33	theme	various	373:379	arg1	microscopy					423:432	scanning electron microscopy	405:432	scanning electron microscopy (SEM)	405:438	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	2	33	theme	various	373:379	arg1	technologies					381:392	various technologies	373:392	various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR)	373:555	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	7	34	theme	adsorption	972:981	arg1	capacity					983:990	The adsorption capacity	968:990	The adsorption capacity	968:990	The adsorption capacity increased with increasing temperature.
26993532	4	35	dep	pH-dependent	745:756	arg1	VI					723:724	VI	723:724	VI	723:724	The results indicate that the adsorption of Cr(VI) on GEC was highly pH-dependent, with the highest adsorption capacity (86.17mg/g) occurring at pH 2.
26993532	4	35	dep	pH-dependent	745:756	arg1	pH-dependent					745:756	pH-dependent	745:756	pH-dependent	745:756	The results indicate that the adsorption of Cr(VI) on GEC was highly pH-dependent, with the highest adsorption capacity (86.17mg/g) occurring at pH 2.
26993532	4	35	dep	pH-dependent	745:756	arg1	adsorption					706:715	the adsorption	702:715	the adsorption of Cr	702:721	The results indicate that the adsorption of Cr(VI) on GEC was highly pH-dependent, with the highest adsorption capacity (86.17mg/g) occurring at pH 2.
26993532	6	36	theme	Freundlich	941:950	arg1	model					961:965	the Freundlich isotherm model	937:965	the Freundlich isotherm model	937:965	The adsorption data were well described by the Freundlich isotherm model.
26993532	8	37	theme	calculated	1035:1044	arg1	parameters					1060:1069	The calculated thermodynamic parameters	1031:1069	The calculated thermodynamic parameters	1031:1069	The calculated thermodynamic parameters indicate that the adsorption is a spontaneous, endothermic and feasible process.
26993532	9	38	theme	adsorption	1200:1209	arg1	capacity					1211:1218	the adsorption capacity	1196:1218	the adsorption capacity of GEC for Cr	1196:1232	The further regeneration experiments showed the adsorption capacity of GEC for Cr(VI) decreased 5% after 7 times reuse, indicating the potential of the as-prepared material for practical application.
26993532	1	39	theme	disodium	273:280	arg1	EDTA-2Na					311:318	EDTA-2Na	311:318	EDTA-2Na	311:318	A novel adsorbent for removal of hexavalent chromium (Cr(VI)) from aqueous solutions has been successfully prepared by modifying graphene oxide/chitosan composite with disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC).
26993532	1	39	theme	disodium	273:280	arg1	GEC					322:324	GEC	322:324	GEC	322:324	A novel adsorbent for removal of hexavalent chromium (Cr(VI)) from aqueous solutions has been successfully prepared by modifying graphene oxide/chitosan composite with disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC).
26993532	1	39	theme	disodium	273:280	arg1	ethylenediaminetetraacetate					282:308	disodium ethylenediaminetetraacetate	273:308	disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC)	273:325	A novel adsorbent for removal of hexavalent chromium (Cr(VI)) from aqueous solutions has been successfully prepared by modifying graphene oxide/chitosan composite with disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC).
26993532	1	40	from	solutions	180:188	arg1	removal					127:133	removal	127:133	removal of hexavalent chromium (Cr(VI)) from aqueous solutions	127:188	A novel adsorbent for removal of hexavalent chromium (Cr(VI)) from aqueous solutions has been successfully prepared by modifying graphene oxide/chitosan composite with disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC).
26993532	4	41	theme	Cr	720:721	arg1	VI					723:724	VI	723:724	VI	723:724	The results indicate that the adsorption of Cr(VI) on GEC was highly pH-dependent, with the highest adsorption capacity (86.17mg/g) occurring at pH 2.
26993532	4	41	theme	Cr	720:721	arg1	pH-dependent					745:756	pH-dependent	745:756	pH-dependent	745:756	The results indicate that the adsorption of Cr(VI) on GEC was highly pH-dependent, with the highest adsorption capacity (86.17mg/g) occurring at pH 2.
26993532	4	41	theme	Cr	720:721	arg1	adsorption					706:715	the adsorption	702:715	the adsorption of Cr	702:721	The results indicate that the adsorption of Cr(VI) on GEC was highly pH-dependent, with the highest adsorption capacity (86.17mg/g) occurring at pH 2.
26993532	3	42	theme	adsorption	564:573	arg1	experiments					575:585	Batch adsorption experiments	558:585	Batch adsorption experiments	558:585	Batch adsorption experiments were carried out to evaluate the adsorption of Cr(VI) by GEC under different conditions.
26993532	8	43	theme	thermodynamic	1046:1058	arg1	parameters					1060:1069	The calculated thermodynamic parameters	1031:1069	The calculated thermodynamic parameters	1031:1069	The calculated thermodynamic parameters indicate that the adsorption is a spontaneous, endothermic and feasible process.
26993532	9	44	theme	GEC	1223:1225	arg1	capacity					1211:1218	the adsorption capacity	1196:1218	the adsorption capacity of GEC for Cr	1196:1232	The further regeneration experiments showed the adsorption capacity of GEC for Cr(VI) decreased 5% after 7 times reuse, indicating the potential of the as-prepared material for practical application.
26993532	9	45	theme	material	1316:1323	arg1	potential					1287:1295	the potential	1283:1295	the potential of the as-prepared material for practical application	1283:1349	The further regeneration experiments showed the adsorption capacity of GEC for Cr(VI) decreased 5% after 7 times reuse, indicating the potential of the as-prepared material for practical application.
26993532	9	46	theme	as-prepared	1304:1314	arg1	material					1316:1323	the as-prepared material	1300:1323	the as-prepared material	1300:1323	The further regeneration experiments showed the adsorption capacity of GEC for Cr(VI) decreased 5% after 7 times reuse, indicating the potential of the as-prepared material for practical application.
26993532	2	47	dep	Fourier	509:515	arg1	transform					517:525	transform	517:525	transform infrared spectroscopy (FT-IR)	517:555	This modified composite was characterized by various technologies; including scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).
26993532	1	48	dep	Cr	159:160	arg1	VI					162:163	VI	162:163	VI	162:163	A novel adsorbent for removal of hexavalent chromium (Cr(VI)) from aqueous solutions has been successfully prepared by modifying graphene oxide/chitosan composite with disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC).
26993532	1	49	theme	aqueous	172:178	arg1	solutions					180:188	aqueous solutions	172:188	aqueous solutions	172:188	A novel adsorbent for removal of hexavalent chromium (Cr(VI)) from aqueous solutions has been successfully prepared by modifying graphene oxide/chitosan composite with disodium ethylenediaminetetraacetate (EDTA-2Na) (GEC).
26993532	3	50	theme	Batch	558:562	arg1	experiments					575:585	Batch adsorption experiments	558:585	Batch adsorption experiments	558:585	Batch adsorption experiments were carried out to evaluate the adsorption of Cr(VI) by GEC under different conditions.
26993532	9	51	dep	showed	1189:1194	arg1	decreased					1238:1246	decreased	1238:1246	decreased	1238:1246	The further regeneration experiments showed the adsorption capacity of GEC for Cr(VI) decreased 5% after 7 times reuse, indicating the potential of the as-prepared material for practical application.
28539265	0	0	theme	oligosaccharide-graft-maleic	54:81	arg1	composite					124:132	chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite	45:132	chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite	45:132	Removal of toxic heavy metal lead (II) using chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite.
28539265	1	1	theme	heavy	335:339	arg1	ion					357:359	the toxic heavy metal lead (II) ion	325:359	the toxic heavy metal lead (II) ion from aqueous solution	325:381	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	2	2	theme	COS-g-MAH/PVA/SF	619:634	arg1	composite					636:644	COS-g-MAH/PVA/SF composite	619:644	COS-g-MAH/PVA/SF composite	619:644	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer has been prepared by utilizing ceric ammonium nitrate as an initiator and the optimised graft copolymer was then used for synthesizing COS-g-MAH/PVA/SF composite.
28539265	8	3	theme	better	1458:1463	arg1	fit					1465:1467	a better fit	1456:1467	a better fit	1456:1467	The adsorption isotherms of Pb(II) could be described very well by Langmuir model and the kinetic results revealed that pseudo second order kinetics shows a better fit.
28539265	0	4	theme	chitosan	45:52	arg1	composite					124:132	chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite	45:132	chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite	45:132	Removal of toxic heavy metal lead (II) using chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite.
28539265	1	5	theme	metal	341:345	arg1	ion					357:359	the toxic heavy metal lead (II) ion	325:359	the toxic heavy metal lead (II) ion from aqueous solution	325:381	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	4	6	theme	FTIR	716:719	arg1	results					721:727	The FTIR results	712:727	The FTIR results	712:727	The FTIR results indicate that the grafted chitosan oligosaccharide copolymer was mixed homogeneously with silk fibroin and polyvinyl alcohol through intermolecular hydrogen bonding.
28539265	2	7	used	used	597:600	arg2	copolymer					578:586	the optimised graft copolymer	558:586	the optimised graft copolymer	558:586	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer has been prepared by utilizing ceric ammonium nitrate as an initiator and the optimised graft copolymer was then used for synthesizing COS-g-MAH/PVA/SF composite.
28539265	1	8	theme	PVA	279:281	arg1	SF					298:299	SF	298:299	SF	298:299	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	8	theme	PVA	279:281	arg1	fibroin					289:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin	195:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies	195:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	0	9	theme	alcohol/silk	103:114	arg1	composite					124:132	chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite	45:132	chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite	45:132	Removal of toxic heavy metal lead (II) using chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite.
28539265	1	10	theme	lead	347:350	arg1	ion					357:359	the toxic heavy metal lead (II) ion	325:359	the toxic heavy metal lead (II) ion from aqueous solution	325:381	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	7	11	theme	composite	1178:1186	arg1	potential					1149:1157	the adsorption potential	1134:1157	the adsorption potential of the synthesized composite	1134:1186	To evaluate the adsorption potential of the synthesized composite, the parameters such as pH, adsorbent dosage, contact time and initial Pb(II)ion concentration was investigated.
28539265	9	12	theme	toxic	1576:1580	arg1	metals					1588:1593	toxic heavy metals	1576:1593	toxic heavy metals	1576:1593	This work provides a practical and high-efficient method for water treatment at moderate concentration of toxic heavy metals.
28539265	6	13	theme	strong	1053:1058	arg1	interaction					1060:1070	a strong interaction	1051:1070	a strong interaction among COS-g-MAH, PVA and silk fibroin components	1051:1119	Both FTIR and XRD results revealed a strong interaction among COS-g-MAH, PVA and silk fibroin components.
28539265	0	14	theme	anhydride/polyvinyl	83:101	arg1	composite					124:132	chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite	45:132	chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite	45:132	Removal of toxic heavy metal lead (II) using chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite.
28539265	8	15	theme	Langmuir	1368:1375	arg1	model					1377:1381	Langmuir model	1368:1381	Langmuir model	1368:1381	The adsorption isotherms of Pb(II) could be described very well by Langmuir model and the kinetic results revealed that pseudo second order kinetics shows a better fit.
28539265	1	16	theme	/silk	283:287	arg1	SF					298:299	SF	298:299	SF	298:299	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	16	theme	/silk	283:287	arg1	fibroin					289:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin	195:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies	195:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	4	17	theme	silk	819:822	arg1	fibroin					824:830	silk fibroin	819:830	silk fibroin	819:830	The FTIR results indicate that the grafted chitosan oligosaccharide copolymer was mixed homogeneously with silk fibroin and polyvinyl alcohol through intermolecular hydrogen bonding.
28539265	5	18	theme	COS-g-MAH/PVA/silk	978:995	arg1	composite					1005:1013	the prepared COS-g-MAH/PVA/silk fibroin composite	965:1013	the prepared COS-g-MAH/PVA/silk fibroin composite	965:1013	The XRD results elucidate the changes in the crystalline behaviour of the prepared COS-g-MAH/PVA/silk fibroin composite.
28539265	9	19	from	concentration	1559:1571	arg1	treatment					1537:1545	water treatment	1531:1545	water treatment at moderate concentration of toxic heavy metals	1531:1593	This work provides a practical and high-efficient method for water treatment at moderate concentration of toxic heavy metals.
28539265	0	20	theme	fibroin	116:122	arg1	composite					124:132	chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite	45:132	chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite	45:132	Removal of toxic heavy metal lead (II) using chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite.
28539265	7	21	theme	synthesized	1166:1176	arg1	composite					1178:1186	the synthesized composite	1162:1186	the synthesized composite	1162:1186	To evaluate the adsorption potential of the synthesized composite, the parameters such as pH, adsorbent dosage, contact time and initial Pb(II)ion concentration was investigated.
28539265	9	22	theme	water	1531:1535	arg1	treatment					1537:1545	water treatment	1531:1545	water treatment at moderate concentration of toxic heavy metals	1531:1593	This work provides a practical and high-efficient method for water treatment at moderate concentration of toxic heavy metals.
28539265	5	23	theme	composite	1005:1013	arg1	behaviour					952:960	the crystalline behaviour	936:960	the crystalline behaviour of the prepared COS-g-MAH/PVA/silk fibroin composite	936:1013	The XRD results elucidate the changes in the crystalline behaviour of the prepared COS-g-MAH/PVA/silk fibroin composite.
28539265	2	24	theme	oligosaccharide-graft-maleic	435:462	arg1	copolymer					474:482	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer	412:482	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer	412:482	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer has been prepared by utilizing ceric ammonium nitrate as an initiator and the optimised graft copolymer was then used for synthesizing COS-g-MAH/PVA/SF composite.
28539265	8	25	theme	order	1435:1439	arg1	kinetics					1441:1448	pseudo second order kinetics	1421:1448	pseudo second order kinetics	1421:1448	The adsorption isotherms of Pb(II) could be described very well by Langmuir model and the kinetic results revealed that pseudo second order kinetics shows a better fit.
28539265	9	26	theme	heavy	1582:1586	arg1	metals					1588:1593	toxic heavy metals	1576:1593	toxic heavy metals	1576:1593	This work provides a practical and high-efficient method for water treatment at moderate concentration of toxic heavy metals.
28539265	4	27	theme	oligosaccharide	764:778	arg1	copolymer					780:788	the grafted chitosan oligosaccharide copolymer	743:788	the grafted chitosan oligosaccharide copolymer	743:788	The FTIR results indicate that the grafted chitosan oligosaccharide copolymer was mixed homogeneously with silk fibroin and polyvinyl alcohol through intermolecular hydrogen bonding.
28539265	9	28	theme	high-efficient	1505:1518	arg1	method					1520:1525	a practical and high-efficient method	1489:1525	a practical and high-efficient method for water treatment at moderate concentration of toxic heavy metals	1489:1593	This work provides a practical and high-efficient method for water treatment at moderate concentration of toxic heavy metals.
28539265	2	29	theme	chitosan	426:433	arg1	copolymer					474:482	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer	412:482	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer	412:482	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer has been prepared by utilizing ceric ammonium nitrate as an initiator and the optimised graft copolymer was then used for synthesizing COS-g-MAH/PVA/SF composite.
28539265	1	30	theme	fibroin	289:295	arg1	efficiency					181:190	the efficiency	177:190	the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies	177:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	3	31	theme	XRD	699:701	arg1	studies					703:709	FTIR and XRD studies	690:709	FTIR and XRD studies	690:709	The prepared samples were analyzed through FTIR and XRD studies.
28539265	1	32	theme	novel	195:199	arg1	SF					298:299	SF	298:299	SF	298:299	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	32	theme	novel	195:199	arg1	fibroin					289:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin	195:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies	195:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	5	33	theme	XRD	899:901	arg1	results					903:909	The XRD results	895:909	The XRD results	895:909	The XRD results elucidate the changes in the crystalline behaviour of the prepared COS-g-MAH/PVA/silk fibroin composite.
28539265	2	34	theme	optimised	562:570	arg1	copolymer					578:586	the optimised graft copolymer	558:586	the optimised graft copolymer	558:586	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer has been prepared by utilizing ceric ammonium nitrate as an initiator and the optimised graft copolymer was then used for synthesizing COS-g-MAH/PVA/SF composite.
28539265	4	35	theme	hydrogen	877:884	arg1	bonding					886:892	intermolecular hydrogen bonding	862:892	intermolecular hydrogen bonding	862:892	The FTIR results indicate that the grafted chitosan oligosaccharide copolymer was mixed homogeneously with silk fibroin and polyvinyl alcohol through intermolecular hydrogen bonding.
28539265	6	36	theme	fibroin	1102:1108	arg1	components					1110:1119	silk fibroin components	1097:1119	silk fibroin components	1097:1119	Both FTIR and XRD results revealed a strong interaction among COS-g-MAH, PVA and silk fibroin components.
28539265	1	37	theme	chitosan	201:208	arg1	SF					298:299	SF	298:299	SF	298:299	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	37	theme	chitosan	201:208	arg1	fibroin					289:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin	195:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies	195:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	4	38	theme	chitosan	755:762	arg1	copolymer					780:788	the grafted chitosan oligosaccharide copolymer	743:788	the grafted chitosan oligosaccharide copolymer	743:788	The FTIR results indicate that the grafted chitosan oligosaccharide copolymer was mixed homogeneously with silk fibroin and polyvinyl alcohol through intermolecular hydrogen bonding.
28539265	6	39	theme	XRD	1030:1032	arg1	results					1034:1040	Both FTIR and XRD results	1016:1040	Both FTIR and XRD results	1016:1040	Both FTIR and XRD results revealed a strong interaction among COS-g-MAH, PVA and silk fibroin components.
28539265	7	40	theme	adsorption	1138:1147	arg1	potential					1149:1157	the adsorption potential	1134:1157	the adsorption potential of the synthesized composite	1134:1186	To evaluate the adsorption potential of the synthesized composite, the parameters such as pH, adsorbent dosage, contact time and initial Pb(II)ion concentration was investigated.
28539265	8	41	theme	kinetic	1391:1397	arg1	results					1399:1405	the kinetic results	1387:1405	the kinetic results	1387:1405	The adsorption isotherms of Pb(II) could be described very well by Langmuir model and the kinetic results revealed that pseudo second order kinetics shows a better fit.
28539265	9	42	theme	moderate	1550:1557	arg1	concentration					1559:1571	moderate concentration	1550:1571	moderate concentration of toxic heavy metals	1550:1593	This work provides a practical and high-efficient method for water treatment at moderate concentration of toxic heavy metals.
28539265	1	43	theme	oligosaccharide-graft-maleic	210:237	arg1	SF					298:299	SF	298:299	SF	298:299	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	43	theme	oligosaccharide-graft-maleic	210:237	arg1	fibroin					289:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin	195:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies	195:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	7	44	theme	initial	1251:1257	arg1	concentration					1269:1281	initial Pb(II)ion concentration	1251:1281	initial Pb(II)ion concentration	1251:1281	To evaluate the adsorption potential of the synthesized composite, the parameters such as pH, adsorbent dosage, contact time and initial Pb(II)ion concentration was investigated.
28539265	8	45	theme	Pb	1329:1330	arg1	isotherms					1316:1324	The adsorption isotherms	1301:1324	The adsorption isotherms of Pb(II)	1301:1334	The adsorption isotherms of Pb(II) could be described very well by Langmuir model and the kinetic results revealed that pseudo second order kinetics shows a better fit.
28539265	1	46	theme	aqueous	366:372	arg1	solution					374:381	aqueous solution	366:381	aqueous solution	366:381	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	4	47	theme	intermolecular	862:875	arg1	bonding					886:892	intermolecular hydrogen bonding	862:892	intermolecular hydrogen bonding	862:892	The FTIR results indicate that the grafted chitosan oligosaccharide copolymer was mixed homogeneously with silk fibroin and polyvinyl alcohol through intermolecular hydrogen bonding.
28539265	1	48	theme	anhydride	239:247	arg1	SF					298:299	SF	298:299	SF	298:299	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	48	theme	anhydride	239:247	arg1	fibroin					289:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin	195:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies	195:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	0	49	theme	metal	23:27	arg1	Removal					0:6	Removal	0:6	Removal of toxic heavy metal lead (II)	0:37	Removal of toxic heavy metal lead (II) using chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite.
28539265	1	50	theme	composite	302:310	arg1	SF					298:299	SF	298:299	SF	298:299	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	50	theme	composite	302:310	arg1	fibroin					289:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin	195:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies	195:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	9	51	theme	practical	1491:1499	arg1	method					1520:1525	a practical and high-efficient method	1489:1525	a practical and high-efficient method for water treatment at moderate concentration of toxic heavy metals	1489:1593	This work provides a practical and high-efficient method for water treatment at moderate concentration of toxic heavy metals.
28539265	7	52	theme	adsorbent	1216:1224	arg1	dosage					1226:1231	adsorbent dosage	1216:1231	adsorbent dosage	1216:1231	To evaluate the adsorption potential of the synthesized composite, the parameters such as pH, adsorbent dosage, contact time and initial Pb(II)ion concentration was investigated.
28539265	2	53	theme	ammonium	521:528	arg1	nitrate					530:536	ceric ammonium nitrate	515:536	ceric ammonium nitrate	515:536	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer has been prepared by utilizing ceric ammonium nitrate as an initiator and the optimised graft copolymer was then used for synthesizing COS-g-MAH/PVA/SF composite.
28539265	2	53	theme	ammonium	521:528	arg1	initiator					544:552	an initiator	541:552	an initiator	541:552	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer has been prepared by utilizing ceric ammonium nitrate as an initiator and the optimised graft copolymer was then used for synthesizing COS-g-MAH/PVA/SF composite.
28539265	1	54	from	solution	374:381	arg1	ion					357:359	the toxic heavy metal lead (II) ion	325:359	the toxic heavy metal lead (II) ion from aqueous solution	325:381	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	8	55	theme	second	1428:1433	arg1	kinetics					1441:1448	pseudo second order kinetics	1421:1448	pseudo second order kinetics	1421:1448	The adsorption isotherms of Pb(II) could be described very well by Langmuir model and the kinetic results revealed that pseudo second order kinetics shows a better fit.
28539265	2	56	theme	anhydride	464:472	arg1	copolymer					474:482	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer	412:482	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer	412:482	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer has been prepared by utilizing ceric ammonium nitrate as an initiator and the optimised graft copolymer was then used for synthesizing COS-g-MAH/PVA/SF composite.
28539265	1	57	theme	batch	386:390	arg1	studies					403:409	batch adsorption studies	386:409	batch adsorption studies	386:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	0	58	dep	metal	23:27	arg1	lead					29:32	lead	29:32	lead	29:32	Removal of toxic heavy metal lead (II) using chitosan oligosaccharide-graft-maleic anhydride/polyvinyl alcohol/silk fibroin composite.
28539265	7	59	theme	Pb	1259:1260	arg1	concentration					1269:1281	initial Pb(II)ion concentration	1251:1281	initial Pb(II)ion concentration	1251:1281	To evaluate the adsorption potential of the synthesized composite, the parameters such as pH, adsorbent dosage, contact time and initial Pb(II)ion concentration was investigated.
28539265	5	60	theme	prepared	969:976	arg1	composite					1005:1013	the prepared COS-g-MAH/PVA/silk fibroin composite	965:1013	the prepared COS-g-MAH/PVA/silk fibroin composite	965:1013	The XRD results elucidate the changes in the crystalline behaviour of the prepared COS-g-MAH/PVA/silk fibroin composite.
28539265	6	61	theme	FTIR	1021:1024	arg1	results					1034:1040	Both FTIR and XRD results	1016:1040	Both FTIR and XRD results	1016:1040	Both FTIR and XRD results revealed a strong interaction among COS-g-MAH, PVA and silk fibroin components.
28539265	1	62	theme	adsorption	392:401	arg1	studies					403:409	batch adsorption studies	386:409	batch adsorption studies	386:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	63	theme	present	139:145	arg1	work					147:150	The present work	135:150	The present work	135:150	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	64	theme	COS-g-MAH	249:257	arg1	SF					298:299	SF	298:299	SF	298:299	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	64	theme	COS-g-MAH	249:257	arg1	fibroin					289:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin	195:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies	195:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	2	65	theme	graft	572:576	arg1	copolymer					578:586	the optimised graft copolymer	558:586	the optimised graft copolymer	558:586	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer has been prepared by utilizing ceric ammonium nitrate as an initiator and the optimised graft copolymer was then used for synthesizing COS-g-MAH/PVA/SF composite.
28539265	3	66	theme	FTIR	690:693	arg1	studies					703:709	FTIR and XRD studies	690:709	FTIR and XRD studies	690:709	The prepared samples were analyzed through FTIR and XRD studies.
28539265	3	67	theme	prepared	651:658	arg1	samples					660:666	The prepared samples	647:666	The prepared samples	647:666	The prepared samples were analyzed through FTIR and XRD studies.
28539265	5	68	from	changes	925:931	arg1	behaviour					952:960	the crystalline behaviour	936:960	the crystalline behaviour of the prepared COS-g-MAH/PVA/silk fibroin composite	936:1013	The XRD results elucidate the changes in the crystalline behaviour of the prepared COS-g-MAH/PVA/silk fibroin composite.
28539265	5	69	theme	fibroin	997:1003	arg1	composite					1005:1013	the prepared COS-g-MAH/PVA/silk fibroin composite	965:1013	the prepared COS-g-MAH/PVA/silk fibroin composite	965:1013	The XRD results elucidate the changes in the crystalline behaviour of the prepared COS-g-MAH/PVA/silk fibroin composite.
28539265	4	70	theme	grafted	747:753	arg1	copolymer					780:788	the grafted chitosan oligosaccharide copolymer	743:788	the grafted chitosan oligosaccharide copolymer	743:788	The FTIR results indicate that the grafted chitosan oligosaccharide copolymer was mixed homogeneously with silk fibroin and polyvinyl alcohol through intermolecular hydrogen bonding.
28539265	2	71	theme	ceric	515:519	arg1	nitrate					530:536	ceric ammonium nitrate	515:536	ceric ammonium nitrate	515:536	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer has been prepared by utilizing ceric ammonium nitrate as an initiator and the optimised graft copolymer was then used for synthesizing COS-g-MAH/PVA/SF composite.
28539265	2	71	theme	ceric	515:519	arg1	initiator					544:552	an initiator	541:552	an initiator	541:552	Initially the chitosan oligosaccharide-graft-maleic anhydride copolymer has been prepared by utilizing ceric ammonium nitrate as an initiator and the optimised graft copolymer was then used for synthesizing COS-g-MAH/PVA/SF composite.
28539265	6	72	theme	silk	1097:1100	arg1	components					1110:1119	silk fibroin components	1097:1119	silk fibroin components	1097:1119	Both FTIR and XRD results revealed a strong interaction among COS-g-MAH, PVA and silk fibroin components.
28539265	9	73	theme	metals	1588:1593	arg1	concentration					1559:1571	moderate concentration	1550:1571	moderate concentration of toxic heavy metals	1550:1593	This work provides a practical and high-efficient method for water treatment at moderate concentration of toxic heavy metals.
28539265	8	74	theme	adsorption	1305:1314	arg1	isotherms					1316:1324	The adsorption isotherms	1301:1324	The adsorption isotherms of Pb(II)	1301:1334	The adsorption isotherms of Pb(II) could be described very well by Langmuir model and the kinetic results revealed that pseudo second order kinetics shows a better fit.
28539265	8	75	theme	pseudo	1421:1426	arg1	kinetics					1441:1448	pseudo second order kinetics	1421:1448	pseudo second order kinetics	1421:1448	The adsorption isotherms of Pb(II) could be described very well by Langmuir model and the kinetic results revealed that pseudo second order kinetics shows a better fit.
28539265	1	76	theme	/Polyvinyl	259:268	arg1	SF					298:299	SF	298:299	SF	298:299	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	76	theme	/Polyvinyl	259:268	arg1	fibroin					289:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin	195:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies	195:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	7	77	theme	ion	1265:1267	arg1	concentration					1269:1281	initial Pb(II)ion concentration	1251:1281	initial Pb(II)ion concentration	1251:1281	To evaluate the adsorption potential of the synthesized composite, the parameters such as pH, adsorbent dosage, contact time and initial Pb(II)ion concentration was investigated.
28539265	1	78	theme	toxic	329:333	arg1	ion					357:359	the toxic heavy metal lead (II) ion	325:359	the toxic heavy metal lead (II) ion from aqueous solution	325:381	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	4	79	theme	polyvinyl	836:844	arg1	alcohol					846:852	polyvinyl alcohol	836:852	polyvinyl alcohol	836:852	The FTIR results indicate that the grafted chitosan oligosaccharide copolymer was mixed homogeneously with silk fibroin and polyvinyl alcohol through intermolecular hydrogen bonding.
28539265	5	80	theme	crystalline	940:950	arg1	behaviour					952:960	the crystalline behaviour	936:960	the crystalline behaviour of the prepared COS-g-MAH/PVA/silk fibroin composite	936:1013	The XRD results elucidate the changes in the crystalline behaviour of the prepared COS-g-MAH/PVA/silk fibroin composite.
28539265	7	81	theme	contact	1234:1240	arg1	time					1242:1245	contact time	1234:1245	contact time	1234:1245	To evaluate the adsorption potential of the synthesized composite, the parameters such as pH, adsorbent dosage, contact time and initial Pb(II)ion concentration was investigated.
28539265	1	82	theme	alcohol	270:276	arg1	SF					298:299	SF	298:299	SF	298:299	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
28539265	1	82	theme	alcohol	270:276	arg1	fibroin					289:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin	195:295	novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies	195:409	The present work was aimed to investigate the efficiency of novel chitosan oligosaccharide-graft-maleic anhydride(COS-g-MAH)/Polyvinyl alcohol (PVA)/silk fibroin (SF) composite for removing the toxic heavy metal lead (II) ion from aqueous solution by batch adsorption studies.
25922852	8	0	theme	fed	943:945	arg1	batch					947:951	fed batch	943:951	fed batch	943:951	16.5 g/L of lactic acid was produced at batch and 22.0 g/L at fed batch.
25922852	10	1	theme	substrate	1124:1132	arg1	inhibition					1134:1143	no substrate inhibition	1121:1143	no substrate inhibition	1121:1143	The cell growth was checked and no substrate inhibition was observed.
25922852	6	2	theme	fructose	730:737	arg1	glucose					717:723	glucose	717:723	glucose	717:723	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	2	theme	fructose	730:737	arg1	fructose					730:737	fructose	730:737	fructose	730:737	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	2	theme	fructose	730:737	arg1	sucrose					708:714	sucrose	708:714	sucrose	708:714	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	2	theme	fructose	730:737	arg1	amounts					684:690	the amounts	680:690	the amounts of lactic acid, sucrose, glucose, and fructose	680:737	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	2	theme	fructose	730:737	arg1	acid					702:705	lactic acid	695:705	lactic acid	695:705	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	9	3	theme	lactic	971:976	arg1	acid					978:981	lactic acid	971:981	lactic acid	971:981	Considering that lactic acid was produced due to the low concentration of the well consumed sugars, the final amount was considerable.
25922852	11	4	theme	L-lactic	1284:1291	arg1	acid					1293:1296	the L-lactic acid	1280:1296	the L-lactic acid	1280:1296	A sucrose molasses hydrolysis is suggested to better avail the molasses fermentation with this strain, surely increasing the L-lactic acid.
25922852	4	5	theme	Batch	474:478	arg1	fermentations					494:506	Batch and fed batch fermentations	474:506	Batch and fed batch fermentations	474:506	Batch and fed batch fermentations were performed with temperature of 43.4°C and pH of 5.0.
25922852	4	6	theme	batch	488:492	arg1	fermentations					494:506	Batch and fed batch fermentations	474:506	Batch and fed batch fermentations	474:506	Batch and fed batch fermentations were performed with temperature of 43.4°C and pH of 5.0.
25922852	1	7	theme	Lactic	64:69	arg1	acid					71:74	Lactic acid	64:74	Lactic acid	64:74	Lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid).
25922852	4	8	theme	fed	484:486	arg1	fermentations					494:506	Batch and fed batch fermentations	474:506	Batch and fed batch fermentations	474:506	Batch and fed batch fermentations were performed with temperature of 43.4°C and pH of 5.0.
25922852	6	9	theme	glucose	717:723	arg1	glucose					717:723	glucose	717:723	glucose	717:723	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	9	theme	glucose	717:723	arg1	fructose					730:737	fructose	730:737	fructose	730:737	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	9	theme	glucose	717:723	arg1	sucrose					708:714	sucrose	708:714	sucrose	708:714	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	9	theme	glucose	717:723	arg1	amounts					684:690	the amounts	680:690	the amounts of lactic acid, sucrose, glucose, and fructose	680:737	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	9	theme	glucose	717:723	arg1	acid					702:705	lactic acid	695:705	lactic acid	695:705	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	3	10	theme	14.0	451:454	arg1	%					455:455	%	455:455	%	455:455	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	11	11	theme	molasses	1222:1229	arg1	fermentation					1231:1242	the molasses fermentation	1218:1242	the molasses fermentation	1218:1242	A sucrose molasses hydrolysis is suggested to better avail the molasses fermentation with this strain, surely increasing the L-lactic acid.
25922852	0	12	theme	L-lactic	0:7	arg1	production					14:23	L-lactic acid production	0:23	L-lactic acid production by Lactobacillus rhamnosus ATCC 10863	0:61	L-lactic acid production by Lactobacillus rhamnosus ATCC 10863.
25922852	2	13	used	used	224:227	arg2	10863					186:190	L. rhamnosus ATCC 10863	168:190	L. rhamnosus ATCC 10863 that produces L-lactic acid	168:218	L. rhamnosus ATCC 10863 that produces L-lactic acid was used to perform the fermentation and molasses was used as substrate.
25922852	1	14	theme	lactic	154:159	arg1	acid					161:164	lactic acid	154:164	lactic acid	154:164	Lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid).
25922852	1	14	theme	lactic	154:159	arg1	poly					149:152	poly	149:152	poly(lactic acid)	149:165	Lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid).
25922852	0	15	theme	acid	9:12	arg1	production					14:23	L-lactic acid production	0:23	L-lactic acid production by Lactobacillus rhamnosus ATCC 10863	0:61	L-lactic acid production by Lactobacillus rhamnosus ATCC 10863.
25922852	6	16	theme	sucrose	708:714	arg1	glucose					717:723	glucose	717:723	glucose	717:723	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	16	theme	sucrose	708:714	arg1	fructose					730:737	fructose	730:737	fructose	730:737	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	16	theme	sucrose	708:714	arg1	sucrose					708:714	sucrose	708:714	sucrose	708:714	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	16	theme	sucrose	708:714	arg1	amounts					684:690	the amounts	680:690	the amounts of lactic acid, sucrose, glucose, and fructose	680:737	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	16	theme	sucrose	708:714	arg1	acid					702:705	lactic acid	695:705	lactic acid	695:705	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	1	17	from	application	118:128	arg1	biomaterials					133:144	biomaterials	133:144	biomaterials	133:144	Lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid).
25922852	3	18	theme	composition	341:351	arg1	molasses					356:363	main composition of molasses	336:363	main composition of molasses	336:363	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	18	theme	composition	341:351	arg1	sucrose					327:333	sucrose	327:333	sucrose (main composition of molasses)	327:364	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	9	19	theme	consumed	1037:1044	arg1	sugars					1046:1051	the well consumed sugars	1028:1051	the well consumed sugars	1028:1051	Considering that lactic acid was produced due to the low concentration of the well consumed sugars, the final amount was considerable.
25922852	4	20	theme	5.0	560:562	arg1	temperature					528:538	temperature	528:538	temperature of 43.4°C	528:548	Batch and fed batch fermentations were performed with temperature of 43.4°C and pH of 5.0.
25922852	4	20	theme	5.0	560:562	arg1	pH					554:555	pH	554:555	pH of 5.0	554:562	Batch and fed batch fermentations were performed with temperature of 43.4°C and pH of 5.0.
25922852	3	21	theme	sucrose	327:333	arg1	g/L					320:322	27.6 g/L	315:322	27.6 g/L of sucrose (main composition of molasses)	315:364	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	21	theme	sucrose	327:333	arg1	g/L					374:376	3.0 g/L	370:376	3.0 g/L of yeast extract	370:393	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	8	22	theme	lactic	893:898	arg1	acid					900:903	lactic acid	893:903	lactic acid	893:903	16.5 g/L of lactic acid was produced at batch and 22.0 g/L at fed batch.
25922852	9	23	theme	low	1007:1009	arg1	concentration					1011:1023	the low concentration	1003:1023	the low concentration of the well consumed sugars	1003:1051	Considering that lactic acid was produced due to the low concentration of the well consumed sugars, the final amount was considerable.
25922852	10	24	theme	cell	1093:1096	arg1	growth					1098:1103	The cell growth	1089:1103	The cell growth	1089:1103	The cell growth was checked and no substrate inhibition was observed.
25922852	0	25	theme	rhamnosus	42:50	arg1	ATCC					52:55	Lactobacillus rhamnosus ATCC 10863	28:61	Lactobacillus rhamnosus ATCC 10863	28:61	L-lactic acid production by Lactobacillus rhamnosus ATCC 10863.
25922852	6	26	theme	acid	702:705	arg1	glucose					717:723	glucose	717:723	glucose	717:723	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	26	theme	acid	702:705	arg1	fructose					730:737	fructose	730:737	fructose	730:737	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	26	theme	acid	702:705	arg1	sucrose					708:714	sucrose	708:714	sucrose	708:714	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	26	theme	acid	702:705	arg1	amounts					684:690	the amounts	680:690	the amounts of lactic acid, sucrose, glucose, and fructose	680:737	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	6	26	theme	acid	702:705	arg1	acid					702:705	lactic acid	695:705	lactic acid	695:705	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	2	27	theme	L-lactic	206:213	arg1	acid					215:218	L-lactic acid	206:218	L-lactic acid	206:218	L. rhamnosus ATCC 10863 that produces L-lactic acid was used to perform the fermentation and molasses was used as substrate.
25922852	3	28	theme	extract	387:393	arg1	g/L					320:322	27.6 g/L	315:322	27.6 g/L of sucrose (main composition of molasses)	315:364	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	28	theme	extract	387:393	arg1	g/L					374:376	3.0 g/L	370:376	3.0 g/L of yeast extract	370:393	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	0	29	theme	Lactobacillus	28:40	arg1	ATCC					52:55	Lactobacillus rhamnosus ATCC 10863	28:61	Lactobacillus rhamnosus ATCC 10863	28:61	L-lactic acid production by Lactobacillus rhamnosus ATCC 10863.
25922852	1	30	contain	have	94:97	arg1	acid					71:74	Lactic acid	64:74	Lactic acid	64:74	Lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid).
25922852	1	30	contain	have	94:97	arg2	application					118:128	the most promising application	99:128	the most promising application in biomaterials	99:144	Lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid).
25922852	8	31	theme	acid	900:903	arg1	g/L					886:888	16.5 g/L	881:888	16.5 g/L of lactic acid	881:903	16.5 g/L of lactic acid was produced at batch and 22.0 g/L at fed batch.
25922852	6	32	theme	lactic	695:700	arg1	acid					702:705	lactic acid	695:705	lactic acid	695:705	Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC.
25922852	4	33	theme	43.4°C	543:548	arg1	temperature					528:538	temperature	528:538	temperature of 43.4°C	528:548	Batch and fed batch fermentations were performed with temperature of 43.4°C and pH of 5.0.
25922852	4	33	theme	43.4°C	543:548	arg1	pH					554:555	pH	554:555	pH of 5.0	554:562	Batch and fed batch fermentations were performed with temperature of 43.4°C and pH of 5.0.
25922852	3	34	theme	of	353:354	arg1	molasses					356:363	main composition of molasses	336:363	main composition of molasses	336:363	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	34	theme	of	353:354	arg1	sucrose					327:333	sucrose	327:333	sucrose (main composition of molasses)	327:364	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	35	contain	containing	304:313	arg2	g/L					320:322	27.6 g/L	315:322	27.6 g/L of sucrose (main composition of molasses)	315:364	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	35	contain	containing	304:313	arg1	solution					295:302	A solution	293:302	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract	293:393	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	35	contain	containing	304:313	arg2	g/L					374:376	3.0 g/L	370:376	3.0 g/L of yeast extract	370:393	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	36	theme	final	425:429	arg1	inoculum					463:470	14.0% (v/v) inoculum	451:470	14.0% (v/v) inoculum	451:470	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	36	theme	final	425:429	arg1	volume					431:436	the final volume	421:436	the final volume of 3,571 mL (14.0% (v/v) inoculum)	421:471	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	2	37	theme	ATCC	181:184	arg1	10863					186:190	L. rhamnosus ATCC 10863	168:190	L. rhamnosus ATCC 10863 that produces L-lactic acid	168:218	L. rhamnosus ATCC 10863 that produces L-lactic acid was used to perform the fermentation and molasses was used as substrate.
25922852	9	38	theme	sugars	1046:1051	arg1	concentration					1011:1023	the low concentration	1003:1023	the low concentration of the well consumed sugars	1003:1051	Considering that lactic acid was produced due to the low concentration of the well consumed sugars, the final amount was considerable.
25922852	3	39	dep	%	455:455	arg1	v/v					458:460	v/v	458:460	v/v	458:460	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	5	40	theme	fed	572:574	arg1	batch					576:580	the fed batch	568:580	the fed batch	568:580	At the fed batch, three molasses feed were applied at 12, 24, and 36 hours.
25922852	2	41	theme	rhamnosus	171:179	arg1	10863					186:190	L. rhamnosus ATCC 10863	168:190	L. rhamnosus ATCC 10863 that produces L-lactic acid	168:218	L. rhamnosus ATCC 10863 that produces L-lactic acid was used to perform the fermentation and molasses was used as substrate.
25922852	1	42	theme	promising	108:116	arg1	application					118:128	the most promising application	99:128	the most promising application in biomaterials	99:144	Lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid).
25922852	3	43	theme	%	455:455	arg1	inoculum					463:470	14.0% (v/v) inoculum	451:470	14.0% (v/v) inoculum	451:470	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	43	theme	%	455:455	arg1	volume					431:436	the final volume	421:436	the final volume of 3,571 mL (14.0% (v/v) inoculum)	421:471	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	2	44	theme	L.	168:169	arg1	10863					186:190	L. rhamnosus ATCC 10863	168:190	L. rhamnosus ATCC 10863 that produces L-lactic acid	168:218	L. rhamnosus ATCC 10863 that produces L-lactic acid was used to perform the fermentation and molasses was used as substrate.
25922852	2	45	used	used	274:277	arg2	substrate					282:290	substrate	282:290	substrate	282:290	L. rhamnosus ATCC 10863 that produces L-lactic acid was used to perform the fermentation and molasses was used as substrate.
25922852	2	45	used	used	274:277	arg2	molasses					261:268	molasses	261:268	molasses	261:268	L. rhamnosus ATCC 10863 that produces L-lactic acid was used to perform the fermentation and molasses was used as substrate.
25922852	7	46	dep	glucose	829:835	arg1	the					825:827	the	825:827	the	825:827	The sucrose was barely consumed at both processes; otherwise the glucose and fructose were almost entirely consumed.
25922852	11	47	theme	sucrose	1161:1167	arg1	hydrolysis					1178:1187	A sucrose molasses hydrolysis	1159:1187	A sucrose molasses hydrolysis	1159:1187	A sucrose molasses hydrolysis is suggested to better avail the molasses fermentation with this strain, surely increasing the L-lactic acid.
25922852	8	48	from	batch	947:951	arg1	batch					921:925	batch	921:925	batch	921:925	16.5 g/L of lactic acid was produced at batch and 22.0 g/L at fed batch.
25922852	8	48	from	batch	947:951	arg1	g/L					936:938	22.0 g/L	931:938	22.0 g/L at fed batch	931:951	16.5 g/L of lactic acid was produced at batch and 22.0 g/L at fed batch.
25922852	3	49	theme	mL	447:448	arg1	inoculum					463:470	14.0% (v/v) inoculum	451:470	14.0% (v/v) inoculum	451:470	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	49	theme	mL	447:448	arg1	volume					431:436	the final volume	421:436	the final volume of 3,571 mL (14.0% (v/v) inoculum)	421:471	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	50	theme	yeast	381:385	arg1	extract					387:393	yeast extract	381:393	yeast extract	381:393	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	11	51	theme	molasses	1169:1176	arg1	hydrolysis					1178:1187	A sucrose molasses hydrolysis	1159:1187	A sucrose molasses hydrolysis	1159:1187	A sucrose molasses hydrolysis is suggested to better avail the molasses fermentation with this strain, surely increasing the L-lactic acid.
25922852	9	52	theme	final	1058:1062	arg1	considerable					1075:1086	considerable	1075:1086	considerable	1075:1086	Considering that lactic acid was produced due to the low concentration of the well consumed sugars, the final amount was considerable.
25922852	9	52	theme	final	1058:1062	arg1	amount					1064:1069	the final amount	1054:1069	the final amount	1054:1069	Considering that lactic acid was produced due to the low concentration of the well consumed sugars, the final amount was considerable.
25922852	3	53	theme	main	336:339	arg1	molasses					356:363	main composition of molasses	336:363	main composition of molasses	336:363	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
25922852	3	53	theme	main	336:339	arg1	sucrose					327:333	sucrose	327:333	sucrose (main composition of molasses)	327:364	A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum).
26400336	13	0	theme	old	1772:1774	arg1	scar					1776:1779	the old scar	1768:1779	the old scar	1768:1779	Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
26400336	9	1	theme	chain	1173:1177	arg1	reaction					1179:1186	real-time polymerase chain reaction	1152:1186	real-time polymerase chain reaction	1152:1186	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	7	2	theme	cavity	833:838	arg1	contents					840:847	marrow cavity contents	826:847	marrow cavity contents	826:847	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	10	3	theme	DCN	1207:1209	arg1	expression					1193:1202	The expression	1189:1202	The expression of DCN and BMP- 2 in different parts of the nonunion area	1189:1260	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	8	4	theme	histological	937:948	arg1	testing					950:956	histological testing	937:956	histological testing	937:956	Through observation and histological testing, component comparisons were made between fracture fragments and surrounding tissue.
26400336	4	5	theme	inherent	547:554	arg1	ability					570:576	inherent anti-fibrotic ability	547:576	inherent anti-fibrotic ability	547:576	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	10	6	theme	highest	1351:1357	arg1	expression					1359:1368	the highest expression	1347:1368	the highest expression of BMP-2	1347:1377	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	2	7	theme	endogenous	260:269	arg1	BMP-2					271:275	endogenous BMP-2	260:275	endogenous BMP-2	260:275	Although the complex role BMP-2 in bone formation is increasingly understood, the role of endogenous BMP-2 in nonunion remains unclear.
26400336	11	8	theme	marrow	1398:1403	arg1	contents					1412:1419	the marrow cavity contents	1394:1419	the marrow cavity contents	1394:1419	Compared with the marrow cavity contents and sticking scars, bone grafts had the highest expression of DCN.
26400336	5	9	theme	Twenty-three	596:607	arg1	patients					609:616	Twenty-three patients	596:616	Twenty-three patients	596:616	Twenty-three patients were selected, including eight cases of delayed union and 15 cases of nonunion.
26400336	7	10	theme	bone	813:816	arg1	grafts					818:823	bone grafts	813:823	bone grafts	813:823	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	13	11	theme	bone	1723:1726	arg1	formation					1728:1736	bone formation	1723:1736	bone formation	1723:1736	Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
26400336	7	12	theme	surrounding	782:792	arg1	scars					863:867	sticking scars	854:867	sticking scars	854:867	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	7	12	theme	surrounding	782:792	arg1	contents					840:847	marrow cavity contents	826:847	marrow cavity contents	826:847	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	7	12	theme	surrounding	782:792	arg1	tissues					794:800	surrounding tissues	782:800	surrounding tissues	782:800	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	7	12	theme	surrounding	782:792	arg1	grafts					818:823	bone grafts	813:823	bone grafts	813:823	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	13	13	theme	scar	1776:1779	arg1	conversion					1754:1763	the conversion	1750:1763	the conversion of the old scar	1750:1779	Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
26400336	10	14	from	expression	1193:1202	arg1	parts					1235:1239	different parts	1225:1239	different parts of the nonunion area	1225:1260	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	12	15	theme	area	1549:1552	arg1	activity					1524:1531	The low antifibrotic and osteogenic activity	1488:1531	The low antifibrotic and osteogenic activity of the nonunion area	1488:1552	The low antifibrotic and osteogenic activity of the nonunion area was associated with non-co-expression of BMP-2 and DCN.
26400336	5	16	theme	union	666:670	arg1	cases					649:653	eight cases	643:653	eight cases of delayed union	643:670	Twenty-three patients were selected, including eight cases of delayed union and 15 cases of nonunion.
26400336	5	16	theme	union	666:670	arg1	cases					679:683	15 cases	676:683	15 cases of nonunion	676:695	Twenty-three patients were selected, including eight cases of delayed union and 15 cases of nonunion.
26400336	10	17	theme	BMP-	1215:1218	arg1	expression					1193:1202	The expression	1189:1202	The expression of DCN and BMP- 2 in different parts of the nonunion area	1189:1260	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	0	18	theme	old	55:57	arg1	fragments					68:76	old fracture fragments	55:76	old fracture fragments	55:76	Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.
26400336	0	19	from	protein	19:25	arg1	fragments					68:76	old fracture fragments	55:76	old fracture fragments	55:76	Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.
26400336	0	19	from	protein	19:25	arg1	tissues					94:100	surrounding tissues	82:100	surrounding tissues	82:100	Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.
26400336	11	20	theme	DCN	1483:1485	arg1	expression					1469:1478	the highest expression	1457:1478	the highest expression of DCN	1457:1485	Compared with the marrow cavity contents and sticking scars, bone grafts had the highest expression of DCN.
26400336	8	21	theme	fracture	999:1006	arg1	fragments					1008:1016	fracture fragments	999:1016	fracture fragments	999:1016	Through observation and histological testing, component comparisons were made between fracture fragments and surrounding tissue.
26400336	9	22	theme	expression	1046:1055	arg1	levels					1057:1062	The expression levels	1042:1062	The expression levels of DCN and BMP-2 in different tissues	1042:1100	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	0	23	theme	surrounding	82:92	arg1	tissues					94:100	surrounding tissues	82:100	surrounding tissues	82:100	Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.
26400336	12	24	theme	low	1492:1494	arg1	activity					1524:1531	The low antifibrotic and osteogenic activity	1488:1531	The low antifibrotic and osteogenic activity of the nonunion area	1488:1552	The low antifibrotic and osteogenic activity of the nonunion area was associated with non-co-expression of BMP-2 and DCN.
26400336	0	25	from	expression	41:50	arg1	fragments					68:76	old fracture fragments	55:76	old fracture fragments	55:76	Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.
26400336	0	25	from	expression	41:50	arg1	tissues					94:100	surrounding tissues	82:100	surrounding tissues	82:100	Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.
26400336	13	26	theme	nonunion	1807:1814	arg1	treatment					1816:1824	better nonunion treatment	1800:1824	better nonunion treatment	1800:1824	Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
26400336	3	27	theme	bone	349:352	arg1	matrix					354:359	bone matrix	349:359	bone matrix	349:359	Decorin (DCN) can promote the formation of bone matrix and calcium deposition to control bone morphogenesis.
26400336	13	28	theme	factor	1652:1657	arg1	BMP					1659:1661	osteogenic factor BMP	1641:1661	osteogenic factor BMP	1641:1661	Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
26400336	10	29	theme	area	1257:1260	arg1	parts					1235:1239	different parts	1225:1239	different parts of the nonunion area	1225:1260	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	0	30	theme	Bone	0:3	arg1	protein					19:25	Bone morphogenetic protein 2	0:27	Bone morphogenetic protein 2	0:27	Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.
26400336	10	31	theme	bone	1289:1292	arg1	graft					1294:1298	bone graft	1289:1298	bone graft	1289:1298	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	1	32	theme	Bone	103:106	arg1	protein					122:128	Bone morphogenetic protein 2	103:130	Bone morphogenetic protein 2 (BMP-2)	103:138	Bone morphogenetic protein 2 (BMP-2) can promote fracture healing.
26400336	1	32	theme	Bone	103:106	arg1	BMP-2					133:137	BMP-2	133:137	BMP-2	133:137	Bone morphogenetic protein 2 (BMP-2) can promote fracture healing.
26400336	3	33	theme	deposition	373:382	arg1	formation					336:344	the formation	332:344	the formation of bone matrix and calcium deposition to control bone morphogenesis	332:412	Decorin (DCN) can promote the formation of bone matrix and calcium deposition to control bone morphogenesis.
26400336	4	34	theme	old	517:519	arg1	zones					530:534	old fracture zones	517:534	old fracture zones	517:534	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	11	35	theme	sticking	1425:1432	arg1	scars					1434:1438	sticking scars	1425:1438	sticking scars	1425:1438	Compared with the marrow cavity contents and sticking scars, bone grafts had the highest expression of DCN.
26400336	9	36	from	BMP-2	1075:1079	arg1	tissues					1094:1100	different tissues	1084:1100	different tissues	1084:1100	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	10	37	theme	cavity	1311:1316	arg1	contents					1318:1325	marrow cavity contents	1304:1325	marrow cavity contents	1304:1325	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	9	38	from	DCN	1067:1069	arg1	tissues					1094:1100	different tissues	1084:1100	different tissues	1084:1100	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	9	39	theme	immunohistochemical	1119:1137	arg1	staining					1139:1146	immunohistochemical staining	1119:1146	immunohistochemical staining	1119:1146	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	4	40	theme	anti-fibrotic	556:568	arg1	ability					570:576	inherent anti-fibrotic ability	547:576	inherent anti-fibrotic ability	547:576	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	9	41	from	levels	1057:1062	arg1	tissues					1094:1100	different tissues	1084:1100	different tissues	1084:1100	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	6	42	theme	Average	698:704	arg1	duration					706:713	Average duration	698:713	Average duration of delayed union or nonunion	698:742	Average duration of delayed union or nonunion was 15 months.
26400336	9	43	theme	polymerase	1162:1171	arg1	reaction					1179:1186	real-time polymerase chain reaction	1152:1186	real-time polymerase chain reaction	1152:1186	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	4	44	theme	zones	530:534	arg1	parts					508:512	different parts	498:512	different parts of old fracture zones	498:534	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	13	45	theme	formation	1728:1736	arg1	induction					1710:1718	the induction	1706:1718	the induction of bone formation	1706:1736	Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
26400336	2	46	theme	BMP-2	271:275	arg1	role					252:255	the role	248:255	the role of endogenous BMP-2 in nonunion	248:287	Although the complex role BMP-2 in bone formation is increasingly understood, the role of endogenous BMP-2 in nonunion remains unclear.
26400336	6	47	theme	union	726:730	arg1	duration					706:713	Average duration	698:713	Average duration of delayed union or nonunion	698:742	Average duration of delayed union or nonunion was 15 months.
26400336	8	48	theme	component	959:967	arg1	comparisons					969:979	component comparisons	959:979	component comparisons	959:979	Through observation and histological testing, component comparisons were made between fracture fragments and surrounding tissue.
26400336	7	49	theme	marrow	826:831	arg1	contents					840:847	marrow cavity contents	826:847	marrow cavity contents	826:847	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	1	50	theme	fracture	152:159	arg1	healing					161:167	fracture healing	152:167	fracture healing	152:167	Bone morphogenetic protein 2 (BMP-2) can promote fracture healing.
26400336	2	51	from	role	191:194	arg1	formation					210:218	bone formation	205:218	bone formation	205:218	Although the complex role BMP-2 in bone formation is increasingly understood, the role of endogenous BMP-2 in nonunion remains unclear.
26400336	6	52	theme	nonunion	735:742	arg1	duration					706:713	Average duration	698:713	Average duration of delayed union or nonunion	698:742	Average duration of delayed union or nonunion was 15 months.
26400336	6	53	theme	delayed	718:724	arg1	union					726:730	union	726:730	union	726:730	Average duration of delayed union or nonunion was 15 months.
26400336	13	54	theme	nonunion	1680:1687	arg1	area					1689:1692	the nonunion area	1676:1692	the nonunion area	1676:1692	Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
26400336	12	55	theme	nonunion	1540:1547	arg1	area					1549:1552	the nonunion area	1536:1552	the nonunion area	1536:1552	The low antifibrotic and osteogenic activity of the nonunion area was associated with non-co-expression of BMP-2 and DCN.
26400336	2	56	theme	bone	205:208	arg1	formation					210:218	bone formation	205:218	bone formation	205:218	Although the complex role BMP-2 in bone formation is increasingly understood, the role of endogenous BMP-2 in nonunion remains unclear.
26400336	1	57	theme	morphogenetic	108:120	arg1	protein					122:128	Bone morphogenetic protein 2	103:130	Bone morphogenetic protein 2 (BMP-2)	103:138	Bone morphogenetic protein 2 (BMP-2) can promote fracture healing.
26400336	1	57	theme	morphogenetic	108:120	arg1	BMP-2					133:137	BMP-2	133:137	BMP-2	133:137	Bone morphogenetic protein 2 (BMP-2) can promote fracture healing.
26400336	10	58	theme	different	1225:1233	arg1	parts					1235:1239	different parts	1225:1239	different parts of the nonunion area	1225:1260	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	10	59	theme	BMP-2	1373:1377	arg1	expression					1359:1368	the highest expression	1347:1368	the highest expression of BMP-2	1347:1377	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	5	60	theme	delayed	658:664	arg1	union					666:670	delayed union	658:670	delayed union	658:670	Twenty-three patients were selected, including eight cases of delayed union and 15 cases of nonunion.
26400336	2	61	from	role	252:255	arg1	nonunion					280:287	nonunion	280:287	nonunion	280:287	Although the complex role BMP-2 in bone formation is increasingly understood, the role of endogenous BMP-2 in nonunion remains unclear.
26400336	12	62	theme	osteogenic	1513:1522	arg1	activity					1524:1531	The low antifibrotic and osteogenic activity	1488:1531	The low antifibrotic and osteogenic activity of the nonunion area	1488:1552	The low antifibrotic and osteogenic activity of the nonunion area was associated with non-co-expression of BMP-2 and DCN.
26400336	11	63	theme	bone	1441:1444	arg1	grafts					1446:1451	bone grafts	1441:1451	bone grafts	1441:1451	Compared with the marrow cavity contents and sticking scars, bone grafts had the highest expression of DCN.
26400336	0	64	theme	fracture	59:66	arg1	fragments					68:76	old fracture fragments	55:76	old fracture fragments	55:76	Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.
26400336	4	65	theme	BMP-2	467:471	arg1	expression					453:462	expression	453:462	expression of BMP-2 and DCN	453:479	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	4	65	theme	BMP-2	467:471	arg1	composition					437:447	tissue composition	430:447	tissue composition	430:447	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	10	66	theme	nonunion	1248:1255	arg1	area					1257:1260	the nonunion area	1244:1260	the nonunion area	1244:1260	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	4	67	theme	DCN	477:479	arg1	expression					453:462	expression	453:462	expression of BMP-2 and DCN	453:479	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	4	67	theme	DCN	477:479	arg1	composition					437:447	tissue composition	430:447	tissue composition	430:447	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	13	68	theme	DCN	1667:1669	arg1	co-injection					1625:1636	the co-injection	1621:1636	the co-injection of osteogenic factor BMP and DCN into the nonunion area	1621:1692	Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
26400336	11	69	theme	highest	1461:1467	arg1	expression					1469:1478	the highest expression	1457:1478	the highest expression of DCN	1457:1485	Compared with the marrow cavity contents and sticking scars, bone grafts had the highest expression of DCN.
26400336	5	70	theme	nonunion	688:695	arg1	cases					649:653	eight cases	643:653	eight cases of delayed union	643:670	Twenty-three patients were selected, including eight cases of delayed union and 15 cases of nonunion.
26400336	5	70	theme	nonunion	688:695	arg1	cases					679:683	15 cases	676:683	15 cases of nonunion	676:695	Twenty-three patients were selected, including eight cases of delayed union and 15 cases of nonunion.
26400336	8	71	theme	surrounding	1022:1032	arg1	tissue					1034:1039	surrounding tissue	1022:1039	surrounding tissue	1022:1039	Through observation and histological testing, component comparisons were made between fracture fragments and surrounding tissue.
26400336	12	72	theme	antifibrotic	1496:1507	arg1	activity					1524:1531	The low antifibrotic and osteogenic activity	1488:1531	The low antifibrotic and osteogenic activity of the nonunion area	1488:1552	The low antifibrotic and osteogenic activity of the nonunion area was associated with non-co-expression of BMP-2 and DCN.
26400336	11	73	theme	cavity	1405:1410	arg1	contents					1412:1419	the marrow cavity contents	1394:1419	the marrow cavity contents	1394:1419	Compared with the marrow cavity contents and sticking scars, bone grafts had the highest expression of DCN.
26400336	13	74	theme	better	1800:1805	arg1	treatment					1816:1824	better nonunion treatment	1800:1824	better nonunion treatment	1800:1824	Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
26400336	13	75	theme	osteogenic	1641:1650	arg1	BMP					1659:1661	osteogenic factor BMP	1641:1661	osteogenic factor BMP	1641:1661	Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
26400336	9	76	theme	DCN	1067:1069	arg1	levels					1057:1062	The expression levels	1042:1062	The expression levels of DCN and BMP-2 in different tissues	1042:1100	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	0	77	theme	morphogenetic	5:17	arg1	protein					19:25	Bone morphogenetic protein 2	0:27	Bone morphogenetic protein 2	0:27	Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.
26400336	4	78	theme	tissue	430:435	arg1	composition					437:447	tissue composition	430:447	tissue composition	430:447	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	3	79	theme	matrix	354:359	arg1	formation					336:344	the formation	332:344	the formation of bone matrix and calcium deposition to control bone morphogenesis	332:412	Decorin (DCN) can promote the formation of bone matrix and calcium deposition to control bone morphogenesis.
26400336	13	80	theme	BMP	1659:1661	arg1	co-injection					1625:1636	the co-injection	1621:1636	the co-injection of osteogenic factor BMP and DCN into the nonunion area	1621:1692	Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
26400336	4	81	located	detected	486:493	arg2	expression					453:462	expression	453:462	expression of BMP-2 and DCN	453:479	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	4	81	located	detected	486:493	arg1	study					423:427	this study	418:427	this study	418:427	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	4	81	located	detected	486:493	arg2	composition					437:447	tissue composition	430:447	tissue composition	430:447	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	4	81	located	detected	486:493	arg1	parts					508:512	different parts	498:512	different parts of old fracture zones	498:534	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	9	82	theme	BMP-2	1075:1079	arg1	levels					1057:1062	The expression levels	1042:1062	The expression levels of DCN and BMP-2 in different tissues	1042:1100	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	3	83	theme	bone	395:398	arg1	morphogenesis					400:412	bone morphogenesis	395:412	bone morphogenesis	395:412	Decorin (DCN) can promote the formation of bone matrix and calcium deposition to control bone morphogenesis.
26400336	12	84	theme	BMP-2	1595:1599	arg1	non-co-expression					1574:1590	non-co-expression	1574:1590	non-co-expression of BMP-2 and DCN	1574:1607	The low antifibrotic and osteogenic activity of the nonunion area was associated with non-co-expression of BMP-2 and DCN.
26400336	4	85	theme	fracture	521:528	arg1	zones					530:534	old fracture zones	517:534	old fracture zones	517:534	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	9	86	theme	different	1084:1092	arg1	tissues					1094:1100	different tissues	1084:1100	different tissues	1084:1100	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	0	87	theme	decorin	33:39	arg1	expression					41:50	decorin expression	33:50	decorin expression	33:50	Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.
26400336	7	88	theme	Fracture	759:766	arg1	scars					863:867	sticking scars	854:867	sticking scars	854:867	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	7	88	theme	Fracture	759:766	arg1	fragments					768:776	Fracture fragments	759:776	Fracture fragments	759:776	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	7	88	theme	Fracture	759:766	arg1	contents					840:847	marrow cavity contents	826:847	marrow cavity contents	826:847	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	7	88	theme	Fracture	759:766	arg1	grafts					818:823	bone grafts	813:823	bone grafts	813:823	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	12	89	theme	DCN	1605:1607	arg1	non-co-expression					1574:1590	non-co-expression	1574:1590	non-co-expression of BMP-2 and DCN	1574:1607	The low antifibrotic and osteogenic activity of the nonunion area was associated with non-co-expression of BMP-2 and DCN.
26400336	9	90	from	tissues	1094:1100	arg1	levels					1057:1062	The expression levels	1042:1062	The expression levels of DCN and BMP-2 in different tissues	1042:1100	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	3	91	theme	calcium	365:371	arg1	deposition					373:382	calcium deposition	365:382	calcium deposition	365:382	Decorin (DCN) can promote the formation of bone matrix and calcium deposition to control bone morphogenesis.
26400336	4	92	theme	different	498:506	arg1	parts					508:512	different parts	498:512	different parts of old fracture zones	498:534	In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis.
26400336	2	93	theme	BMP-2	196:200	arg1	role					191:194	the complex role BMP-2	179:200	the complex role BMP-2 in bone formation	179:218	Although the complex role BMP-2 in bone formation is increasingly understood, the role of endogenous BMP-2 in nonunion remains unclear.
26400336	10	94	theme	marrow	1304:1309	arg1	contents					1318:1325	marrow cavity contents	1304:1325	marrow cavity contents	1304:1325	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	10	95	contain	had	1343:1345	arg1	scars					1337:1341	sticking scars	1328:1341	sticking scars	1328:1341	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	10	95	contain	had	1343:1345	arg2	expression					1359:1368	the highest expression	1347:1368	the highest expression of BMP-2	1347:1377	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
26400336	9	96	theme	real-time	1152:1160	arg1	reaction					1179:1186	real-time polymerase chain reaction	1152:1186	real-time polymerase chain reaction	1152:1186	The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction.
26400336	2	97	theme	complex	183:189	arg1	role					191:194	the complex role BMP-2	179:200	the complex role BMP-2 in bone formation	179:218	Although the complex role BMP-2 in bone formation is increasingly understood, the role of endogenous BMP-2 in nonunion remains unclear.
26400336	7	98	theme	sticking	854:861	arg1	scars					863:867	sticking scars	854:867	sticking scars	854:867	Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery.
26400336	11	99	contain	had	1453:1455	arg2	expression					1469:1478	the highest expression	1457:1478	the highest expression of DCN	1457:1485	Compared with the marrow cavity contents and sticking scars, bone grafts had the highest expression of DCN.
26400336	11	99	contain	had	1453:1455	arg1	grafts					1446:1451	bone grafts	1441:1451	bone grafts	1441:1451	Compared with the marrow cavity contents and sticking scars, bone grafts had the highest expression of DCN.
26400336	10	100	theme	sticking	1328:1335	arg1	scars					1337:1341	sticking scars	1328:1341	sticking scars	1328:1341	The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2.
24552302	9	0	theme	difra	1612:1616	arg1	formulations					1625:1636	sorghum and difra starch formulations	1600:1636	formulations	1625:1636	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	4	1	theme	factorial	910:918	arg1	studies					933:939	two factorial experimental studies	906:939	two factorial experimental studies	906:939	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments was evaluated in two factorial experimental studies.
24552302	8	2	contain	containing	1462:1471	arg2	starch					1499:1504	sorghum, maize, and difra starch	1473:1504	sorghum, maize, and difra starch	1473:1504	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	8	2	contain	containing	1462:1471	arg1	mixtures					1453:1460	co-processed mixtures	1440:1460	co-processed mixtures containing sorghum, maize, and difra starch	1440:1504	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	3	3	theme	tablets	730:736	arg1	properties					695:704	physical properties	686:704	physical properties of paracetamol (250 mg) tablets	686:736	MATERIALS AND METHODS Three experimental design studies were performed to determine the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets.
24552302	12	4	theme	Difra	2024:2028	arg1	starch					2030:2035	CONCLUSION Difra starch	2013:2035	CONCLUSION Difra starch	2013:2035	CONCLUSION Difra starch can replace maize and sorghum starch as tablet excipient.
24552302	4	5	theme	preceding	867:875	arg1	experiments					877:887	the preceding experiments	863:887	the preceding experiments	863:887	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments was evaluated in two factorial experimental studies.
24552302	3	6	theme	250 mg	722:727	arg1	tablets					730:736	paracetamol (250 mg) tablets	709:736	paracetamol (250 mg) tablets	709:736	MATERIALS AND METHODS Three experimental design studies were performed to determine the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets.
24552302	10	7	contain	containing	1880:1889	arg1	tablets					1872:1878	tablets	1872:1878	tablets containing maize and difra starch	1872:1912	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	10	7	contain	containing	1880:1889	arg2	starch					1907:1912	maize and difra starch	1891:1912	maize and difra starch	1891:1912	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	3	8	theme	experimental	582:593	arg1	studies					602:608	Three experimental design studies	576:608	MATERIALS AND METHODS Three experimental design studies	554:608	MATERIALS AND METHODS Three experimental design studies were performed to determine the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets.
24552302	1	9	theme	botanical	175:183	arg1	sources					185:191	different botanical sources	165:191	different botanical sources	165:191	CONTEXT Starch obtained from different botanical sources exhibit different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions.
24552302	9	10	theme	higher	1670:1675	arg1	capacity					1687:1694	their higher hydration capacity	1664:1694	their higher hydration capacity (difra > sorghum > maize starch)	1664:1727	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	9	10	theme	higher	1670:1675	arg1	starch					1721:1726	difra > sorghum > maize starch	1697:1726	difra > sorghum > maize starch	1697:1726	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	11	11	theme	vitro	1986:1990	arg1	studies					2004:2010	vitro dissolution studies	1986:2010	vitro dissolution studies	1986:2010	However, more than 90% drug was released in vitro dissolution studies.
24552302	8	12	theme	mixtures	1453:1460	arg1	1.19					1511:1514	1.19	1511:1514	1.19	1511:1514	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	8	12	theme	mixtures	1453:1460	arg1	ratios					1430:1435	The Hausner ratios	1418:1435	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch	1418:1504	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	12	13	theme	sorghum	2059:2065	arg1	starch					2067:2072	sorghum starch	2059:2072	sorghum starch	2059:2072	CONCLUSION Difra starch can replace maize and sorghum starch as tablet excipient.
24552302	8	14	theme	sorghum	1473:1479	arg1	starch					1499:1504	sorghum, maize, and difra starch	1473:1504	sorghum, maize, and difra starch	1473:1504	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	6	15	dep	starch	1233:1238	arg1	30:9					1254:1257	30:9	1254:1257	30:9	1254:1257	RESULTS Tablets prepared from maize starch and povidone (30:9, w/w) blend which was previously mixed for 8 min disintegrated (DT) in 10 min.
24552302	6	15	dep	starch	1233:1238	arg1	w/w					1260:1262	w/w	1260:1262	w/w	1260:1262	RESULTS Tablets prepared from maize starch and povidone (30:9, w/w) blend which was previously mixed for 8 min disintegrated (DT) in 10 min.
24552302	9	16	with	2.5 min	1767:1773	arg1	friability					1797:1806	a friability	1795:1806	a friability of 0.32%	1795:1815	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	9	17	theme	formulations	1625:1636	arg1	1.5 min					1755:1761	1.5 min	1755:1761	1.5 min	1755:1761	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	9	17	theme	formulations	1625:1636	arg1	DT					1594:1595	The DT	1590:1595	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property	1590:1749	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	8	18	theme	maize	1482:1486	arg1	starch					1499:1504	sorghum, maize, and difra starch	1473:1504	sorghum, maize, and difra starch	1473:1504	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	6	19	theme	povidone	1244:1251	arg1	blend					1265:1269	maize starch and povidone (30:9, w/w) blend	1227:1269	maize starch and povidone (30:9, w/w) blend which was previously mixed for 8 min	1227:1306	RESULTS Tablets prepared from maize starch and povidone (30:9, w/w) blend which was previously mixed for 8 min disintegrated (DT) in 10 min.
24552302	2	20	dep	OBJECTIVE	332:340	arg1	characterized					360:372	characterized	360:372	characterized Echinochloa colona (L.) Link (Poaceae) starch	360:418	OBJECTIVE The present study characterized Echinochloa colona (L.) Link (Poaceae) starch and evaluated its compressional characteristics for use as tablet excipient against commonly used maize, sorghum, and cassava starch.
24552302	2	20	dep	OBJECTIVE	332:340	arg1	evaluated					424:432	evaluated	424:432	evaluated its compressional characteristics for use as tablet excipient against commonly used maize, sorghum, and cassava starch	424:551	OBJECTIVE The present study characterized Echinochloa colona (L.) Link (Poaceae) starch and evaluated its compressional characteristics for use as tablet excipient against commonly used maize, sorghum, and cassava starch.
24552302	9	21	with	1.5 min	1755:1761	arg1	friability					1797:1806	a friability	1795:1806	a friability of 0.32%	1795:1815	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	3	22	dep	MATERIALS	554:562	arg1	studies					602:608	Three experimental design studies	576:608	MATERIALS AND METHODS Three experimental design studies	554:608	MATERIALS AND METHODS Three experimental design studies were performed to determine the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets.
24552302	9	23	theme	swelling	1733:1740	arg1	property					1742:1749	swelling property	1733:1749	swelling property	1733:1749	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	6	24	theme	starch	1233:1238	arg1	blend					1265:1269	maize starch and povidone (30:9, w/w) blend	1227:1269	maize starch and povidone (30:9, w/w) blend which was previously mixed for 8 min	1227:1306	RESULTS Tablets prepared from maize starch and povidone (30:9, w/w) blend which was previously mixed for 8 min disintegrated (DT) in 10 min.
24552302	0	25	theme	starch	70:75	arg1	binding					12:18	Comparative binding	0:18	Comparative binding	0:18	Comparative binding and disintegrating property of Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch.
24552302	0	25	theme	starch	70:75	arg1	property					39:46	disintegrating property	24:46	disintegrating property	24:46	Comparative binding and disintegrating property of Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch.
24552302	0	26	theme	difra	78:82	arg1	starch					70:75	Echinochloa colona starch	51:75	Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch	51:133	Comparative binding and disintegrating property of Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch.
24552302	0	26	theme	difra	78:82	arg1	starch					84:89	difra starch	78:89	difra starch	78:89	Comparative binding and disintegrating property of Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch.
24552302	3	27	theme	maize	657:661	arg1	starch					663:668	the maize starch	653:668	the maize starch	653:668	MATERIALS AND METHODS Three experimental design studies were performed to determine the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets.
24552302	4	28	theme	starch	779:784	arg1	glycolate					786:794	superdisintegrants sodium starch glycolate	753:794	superdisintegrants sodium starch glycolate	753:794	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments was evaluated in two factorial experimental studies.
24552302	11	29	dep	%	1963:1963	arg1	90					1961:1962	90	1961:1962	90	1961:1962	However, more than 90% drug was released in vitro dissolution studies.
24552302	5	30	theme	lubrication	1137:1147	arg1	sensitivity					1149:1159	lubrication sensitivity	1137:1159	lubrication sensitivity	1137:1159	The maize starch in the optimum formulations was replaced with difra, sorghum, and cassava starch, and tablets prepared from these starches were compared for their compressional characteristics, lubrication sensitivity, moisture uptake, and drug release.
24552302	3	31	from	effects	642:648	arg1	properties					695:704	physical properties	686:704	physical properties of paracetamol (250 mg) tablets	686:736	MATERIALS AND METHODS Three experimental design studies were performed to determine the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets.
24552302	0	32	theme	Comparative	0:10	arg1	binding					12:18	Comparative binding	0:18	Comparative binding	0:18	Comparative binding and disintegrating property of Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch.
24552302	4	33	from	effect	743:748	arg1	composition					839:849	the optimized composition	825:849	the optimized composition obtained in the preceding experiments	825:887	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments was evaluated in two factorial experimental studies.
24552302	2	34	theme	compressional	438:450	arg1	characteristics					452:466	its compressional characteristics	434:466	its compressional characteristics for use as tablet excipient against commonly used maize, sorghum, and cassava starch	434:551	OBJECTIVE The present study characterized Echinochloa colona (L.) Link (Poaceae) starch and evaluated its compressional characteristics for use as tablet excipient against commonly used maize, sorghum, and cassava starch.
24552302	4	35	theme	superdisintegrants	753:770	arg1	glycolate					786:794	superdisintegrants sodium starch glycolate	753:794	superdisintegrants sodium starch glycolate	753:794	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments was evaluated in two factorial experimental studies.
24552302	1	36	theme	different	291:299	arg1	interactions					318:329	different binder substrate interactions	291:329	different binder substrate interactions	291:329	CONTEXT Starch obtained from different botanical sources exhibit different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions.
24552302	3	37	theme	starch	663:668	arg1	effects					642:648	the effects	638:648	the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets	638:736	MATERIALS AND METHODS Three experimental design studies were performed to determine the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets.
24552302	2	38	theme	starch	413:418	arg1	Echinochloa					374:384	Echinochloa	374:384	Echinochloa colona (L.) Link (Poaceae) starch	374:418	OBJECTIVE The present study characterized Echinochloa colona (L.) Link (Poaceae) starch and evaluated its compressional characteristics for use as tablet excipient against commonly used maize, sorghum, and cassava starch.
24552302	1	39	theme	substrate	308:316	arg1	interactions					318:329	different binder substrate interactions	291:329	different binder substrate interactions	291:329	CONTEXT Starch obtained from different botanical sources exhibit different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions.
24552302	9	40	theme	%	1815:1815	arg1	friability					1797:1806	a friability	1795:1806	a friability of 0.32%	1795:1815	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	8	41	theme	equilibrium	1552:1562	arg1	content					1573:1579	equilibrium moisture content	1552:1579	equilibrium moisture content of 8-9%	1552:1587	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	0	42	theme	Echinochloa	51:61	arg1	starch					70:75	Echinochloa colona starch	51:75	Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch	51:133	Comparative binding and disintegrating property of Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch.
24552302	0	42	theme	Echinochloa	51:61	arg1	starch					84:89	difra starch	78:89	difra starch	78:89	Comparative binding and disintegrating property of Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch.
24552302	3	43	theme	physical	686:693	arg1	properties					695:704	physical properties	686:704	physical properties of paracetamol (250 mg) tablets	686:736	MATERIALS AND METHODS Three experimental design studies were performed to determine the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets.
24552302	4	44	theme	sodium	815:820	arg1	effect					743:748	The effect	739:748	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments	739:887	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments was evaluated in two factorial experimental studies.
24552302	2	45	theme	present	346:352	arg1	study					354:358	The present study	342:358	The present study	342:358	OBJECTIVE The present study characterized Echinochloa colona (L.) Link (Poaceae) starch and evaluated its compressional characteristics for use as tablet excipient against commonly used maize, sorghum, and cassava starch.
24552302	1	46	theme	due	227:229	arg1	characteristics					211:225	different characteristics	201:225	different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions	201:329	CONTEXT Starch obtained from different botanical sources exhibit different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions.
24552302	3	47	theme	povidone	674:681	arg1	effects					642:648	the effects	638:648	the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets	638:736	MATERIALS AND METHODS Three experimental design studies were performed to determine the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets.
24552302	8	48	theme	co-processed	1440:1451	arg1	mixtures					1453:1460	co-processed mixtures	1440:1460	co-processed mixtures containing sorghum, maize, and difra starch	1440:1504	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	10	49	dep	DT	1851:1852	arg1	the					1847:1849	the	1847:1849	the	1847:1849	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	10	50	theme	tablets	1872:1878	arg1	friability					1858:1867	friability	1858:1867	friability	1858:1867	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	10	50	theme	tablets	1872:1878	arg1	DT					1851:1852	DT	1851:1852	DT	1851:1852	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	1	51	theme	amylase-amylopectin	247:265	arg1	ratio					267:271	amylase-amylopectin ratio	247:271	amylase-amylopectin ratio	247:271	CONTEXT Starch obtained from different botanical sources exhibit different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions.
24552302	9	52	theme	starch	1618:1623	arg1	formulations					1625:1636	sorghum and difra starch formulations	1600:1636	formulations	1625:1636	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	10	53	theme	difra	1901:1905	arg1	starch					1907:1912	maize and difra starch	1891:1912	maize and difra starch	1891:1912	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	10	54	theme	maize	1891:1895	arg1	starch					1907:1912	maize and difra starch	1891:1912	maize and difra starch	1891:1912	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	1	55	theme	different	165:173	arg1	sources					185:191	different botanical sources	165:191	different botanical sources	165:191	CONTEXT Starch obtained from different botanical sources exhibit different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions.
24552302	6	56	dep	disintegrated	1308:1320	arg1	DT					1323:1324	DT	1323:1324	DT	1323:1324	RESULTS Tablets prepared from maize starch and povidone (30:9, w/w) blend which was previously mixed for 8 min disintegrated (DT) in 10 min.
24552302	12	57	theme	tablet	2077:2082	arg1	excipient					2084:2092	tablet excipient	2077:2092	tablet excipient	2077:2092	CONCLUSION Difra starch can replace maize and sorghum starch as tablet excipient.
24552302	7	58	dep	decreased	1357:1365	arg1	p < 0.05					1396:1403	p < 0.05	1396:1403	p < 0.05	1396:1403	Superdisintegrants decreased DT of tablets significantly (p < 0.05) to 2.2 min.
24552302	9	59	theme	hydration	1677:1685	arg1	capacity					1687:1694	their higher hydration capacity	1664:1694	their higher hydration capacity (difra > sorghum > maize starch)	1664:1727	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	9	59	theme	hydration	1677:1685	arg1	starch					1721:1726	difra > sorghum > maize starch	1697:1726	difra > sorghum > maize starch	1697:1726	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	6	60	dep	RESULTS	1197:1203	arg1	Tablets					1205:1211	Tablets	1205:1211	RESULTS Tablets prepared from maize starch and povidone (30:9, w/w) blend which was previously mixed for 8 min	1197:1306	RESULTS Tablets prepared from maize starch and povidone (30:9, w/w) blend which was previously mixed for 8 min disintegrated (DT) in 10 min.
24552302	0	61	theme	cassava	120:126	arg1	starch					128:133	cassava starch	120:133	cassava starch	120:133	Comparative binding and disintegrating property of Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch.
24552302	1	62	theme	different	201:209	arg1	characteristics					211:225	different characteristics	201:225	different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions	201:329	CONTEXT Starch obtained from different botanical sources exhibit different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions.
24552302	2	63	theme	cassava	538:544	arg1	starch					546:551	cassava starch	538:551	cassava starch	538:551	OBJECTIVE The present study characterized Echinochloa colona (L.) Link (Poaceae) starch and evaluated its compressional characteristics for use as tablet excipient against commonly used maize, sorghum, and cassava starch.
24552302	1	64	from	variation	234:242	arg1	ratio					267:271	amylase-amylopectin ratio	247:271	amylase-amylopectin ratio	247:271	CONTEXT Starch obtained from different botanical sources exhibit different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions.
24552302	5	65	theme	compressional	1106:1118	arg1	characteristics					1120:1134	their compressional characteristics	1100:1134	their compressional characteristics	1100:1134	The maize starch in the optimum formulations was replaced with difra, sorghum, and cassava starch, and tablets prepared from these starches were compared for their compressional characteristics, lubrication sensitivity, moisture uptake, and drug release.
24552302	3	66	theme	design	595:600	arg1	studies					602:608	Three experimental design studies	576:608	MATERIALS AND METHODS Three experimental design studies	554:608	MATERIALS AND METHODS Three experimental design studies were performed to determine the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets.
24552302	5	67	theme	maize	946:950	arg1	starch					952:957	The maize starch	942:957	The maize starch in the optimum formulations	942:985	The maize starch in the optimum formulations was replaced with difra, sorghum, and cassava starch, and tablets prepared from these starches were compared for their compressional characteristics, lubrication sensitivity, moisture uptake, and drug release.
24552302	4	68	theme	experimental	920:931	arg1	studies					933:939	two factorial experimental studies	906:939	two factorial experimental studies	906:939	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments was evaluated in two factorial experimental studies.
24552302	12	69	theme	CONCLUSION	2013:2022	arg1	starch					2030:2035	CONCLUSION Difra starch	2013:2035	CONCLUSION Difra starch	2013:2035	CONCLUSION Difra starch can replace maize and sorghum starch as tablet excipient.
24552302	8	70	theme	difra	1493:1497	arg1	starch					1499:1504	sorghum, maize, and difra starch	1473:1504	sorghum, maize, and difra starch	1473:1504	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	7	71	theme	tablets	1373:1379	arg1	DT					1367:1368	DT	1367:1368	DT of tablets	1367:1379	Superdisintegrants decreased DT of tablets significantly (p < 0.05) to 2.2 min.
24552302	5	72	dep	difra	1005:1009	arg1	starch					1033:1038	starch	1033:1038	starch	1033:1038	The maize starch in the optimum formulations was replaced with difra, sorghum, and cassava starch, and tablets prepared from these starches were compared for their compressional characteristics, lubrication sensitivity, moisture uptake, and drug release.
24552302	11	73	dep	drug	1965:1968	arg1	%					1963:1963	%	1963:1963	%	1963:1963	However, more than 90% drug was released in vitro dissolution studies.
24552302	2	74	theme	used	513:516	arg1	maize					518:522	commonly used maize	504:522	commonly used maize	504:522	OBJECTIVE The present study characterized Echinochloa colona (L.) Link (Poaceae) starch and evaluated its compressional characteristics for use as tablet excipient against commonly used maize, sorghum, and cassava starch.
24552302	6	75	theme	maize	1227:1231	arg1	starch					1233:1238	maize starch	1227:1238	maize starch	1227:1238	RESULTS Tablets prepared from maize starch and povidone (30:9, w/w) blend which was previously mixed for 8 min disintegrated (DT) in 10 min.
24552302	5	76	theme	moisture	1162:1169	arg1	uptake					1171:1176	moisture uptake	1162:1176	moisture uptake	1162:1176	The maize starch in the optimum formulations was replaced with difra, sorghum, and cassava starch, and tablets prepared from these starches were compared for their compressional characteristics, lubrication sensitivity, moisture uptake, and drug release.
24552302	1	77	theme	CONTEXT	136:142	arg1	Starch					144:149	CONTEXT Starch	136:149	CONTEXT Starch obtained from different botanical sources	136:191	CONTEXT Starch obtained from different botanical sources exhibit different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions.
24552302	4	78	theme	croscarmellose	800:813	arg1	sodium					815:820	croscarmellose sodium	800:820	croscarmellose sodium	800:820	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments was evaluated in two factorial experimental studies.
24552302	10	79	dep	significant	1918:1928	arg1	p < 0.05					1931:1938	p < 0.05	1931:1938	p < 0.05	1931:1938	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	10	79	dep	significant	1918:1928	arg1	effect					1822:1827	The effect	1818:1827	The effect of lubrication on the DT and friability of tablets containing maize and difra starch	1818:1912	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	10	79	dep	significant	1918:1928	arg1	significant					1918:1928	significant	1918:1928	significant	1918:1928	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	4	80	theme	sodium	772:777	arg1	glycolate					786:794	superdisintegrants sodium starch glycolate	753:794	superdisintegrants sodium starch glycolate	753:794	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments was evaluated in two factorial experimental studies.
24552302	4	81	theme	glycolate	786:794	arg1	effect					743:748	The effect	739:748	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments	739:887	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments was evaluated in two factorial experimental studies.
24552302	5	82	theme	optimum	966:972	arg1	formulations					974:985	the optimum formulations	962:985	the optimum formulations	962:985	The maize starch in the optimum formulations was replaced with difra, sorghum, and cassava starch, and tablets prepared from these starches were compared for their compressional characteristics, lubrication sensitivity, moisture uptake, and drug release.
24552302	0	83	theme	disintegrating	24:37	arg1	property					39:46	disintegrating property	24:46	disintegrating property	24:46	Comparative binding and disintegrating property of Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch.
24552302	5	84	from	starch	952:957	arg1	formulations					974:985	the optimum formulations	962:985	the optimum formulations	962:985	The maize starch in the optimum formulations was replaced with difra, sorghum, and cassava starch, and tablets prepared from these starches were compared for their compressional characteristics, lubrication sensitivity, moisture uptake, and drug release.
24552302	10	85	theme	lubrication	1832:1842	arg1	p < 0.05					1931:1938	p < 0.05	1931:1938	p < 0.05	1931:1938	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	10	85	theme	lubrication	1832:1842	arg1	effect					1822:1827	The effect	1818:1827	The effect of lubrication on the DT and friability of tablets containing maize and difra starch	1818:1912	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	10	85	theme	lubrication	1832:1842	arg1	significant					1918:1928	significant	1918:1928	significant	1918:1928	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	1	86	theme	binder	301:306	arg1	interactions					318:329	different binder substrate interactions	291:329	different binder substrate interactions	291:329	CONTEXT Starch obtained from different botanical sources exhibit different characteristics due to variation in amylase-amylopectin ratio, which results in different binder substrate interactions.
24552302	2	87	theme	tablet	479:484	arg1	excipient					486:494	tablet excipient	479:494	tablet excipient	479:494	OBJECTIVE The present study characterized Echinochloa colona (L.) Link (Poaceae) starch and evaluated its compressional characteristics for use as tablet excipient against commonly used maize, sorghum, and cassava starch.
24552302	8	88	theme	moisture	1564:1571	arg1	content					1573:1579	equilibrium moisture content	1552:1579	equilibrium moisture content of 8-9%	1552:1587	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	4	89	theme	optimized	829:837	arg1	composition					839:849	the optimized composition	825:849	the optimized composition obtained in the preceding experiments	825:887	The effect of superdisintegrants sodium starch glycolate and croscarmellose sodium on the optimized composition obtained in the preceding experiments was evaluated in two factorial experimental studies.
24552302	0	90	theme	colona	63:68	arg1	starch					70:75	Echinochloa colona starch	51:75	Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch	51:133	Comparative binding and disintegrating property of Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch.
24552302	0	90	theme	colona	63:68	arg1	starch					84:89	difra starch	78:89	difra starch	78:89	Comparative binding and disintegrating property of Echinochloa colona starch (difra starch) against maize, sorghum, and cassava starch.
24552302	5	91	theme	drug	1183:1186	arg1	release					1188:1194	drug release	1183:1194	drug release	1183:1194	The maize starch in the optimum formulations was replaced with difra, sorghum, and cassava starch, and tablets prepared from these starches were compared for their compressional characteristics, lubrication sensitivity, moisture uptake, and drug release.
24552302	11	92	theme	dissolution	1992:2002	arg1	studies					2004:2010	vitro dissolution studies	1986:2010	vitro dissolution studies	1986:2010	However, more than 90% drug was released in vitro dissolution studies.
24552302	8	93	theme	%	1587:1587	arg1	content					1573:1579	equilibrium moisture content	1552:1579	equilibrium moisture content of 8-9%	1552:1587	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	8	94	theme	Hausner	1422:1428	arg1	1.19					1511:1514	1.19	1511:1514	1.19	1511:1514	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	8	94	theme	Hausner	1422:1428	arg1	ratios					1430:1435	The Hausner ratios	1418:1435	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch	1418:1504	The Hausner ratios of co-processed mixtures containing sorghum, maize, and difra starch were 1.19, 1.21, and 1.26, respectively, with equilibrium moisture content of 8-9%.
24552302	10	95	from	effect	1822:1827	arg1	friability					1858:1867	friability	1858:1867	friability	1858:1867	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	10	95	from	effect	1822:1827	arg1	DT					1851:1852	DT	1851:1852	DT	1851:1852	The effect of lubrication on the DT and friability of tablets containing maize and difra starch was significant (p < 0.05).
24552302	9	96	theme	sorghum	1600:1606	arg1	formulations					1625:1636	sorghum and difra starch formulations	1600:1636	formulations	1625:1636	The DT of sorghum and difra starch formulations which related directly to their higher hydration capacity (difra > sorghum > maize starch) and swelling property was 1.5 min and 2.5 min, respectively, with a friability of 0.32%.
24552302	3	97	theme	paracetamol	709:719	arg1	tablets					730:736	paracetamol (250 mg) tablets	709:736	paracetamol (250 mg) tablets	709:736	MATERIALS AND METHODS Three experimental design studies were performed to determine the effects of the maize starch and povidone on physical properties of paracetamol (250 mg) tablets.
26192070	4	0	theme	CaCO3	588:592	arg1	suspension					564:573	the colloidal suspension	550:573	the colloidal suspension of amorphous CaCO3 (ACC) at room temperature	550:618	The crystallization of CaCO3 is conducted by soaking the templates in the colloidal suspension of amorphous CaCO3 (ACC) at room temperature.
26192070	0	1	theme	Biomineralization	91:107	arg1	Processes					109:117	the Biomineralization Processes	87:117	the Biomineralization Processes of Crustacean Cuticles	87:140	Formation of Helically Structured Chitin/CaCO3 Hybrids through an Approach Inspired by the Biomineralization Processes of Crustacean Cuticles.
26192070	5	2	theme	CaCO3	737:741	arg1	nanocrystals					743:754	CaCO3 nanocrystals	737:754	CaCO3 nanocrystals	737:754	At the initial stage of crystallization, ACC particles are introduced inside the templates, and they crystallize to CaCO3 nanocrystals.
26192070	4	3	theme	CaCO3	503:507	arg1	crystallization					484:498	The crystallization	480:498	The crystallization of CaCO3	480:507	The crystallization of CaCO3 is conducted by soaking the templates in the colloidal suspension of amorphous CaCO3 (ACC) at room temperature.
26192070	6	4	theme	CaCO3	792:796	arg1	crystallization					798:812	CaCO3 crystallization	792:812	CaCO3 crystallization	792:812	The acidic polymer networks induce CaCO3 crystallization.
26192070	7	5	theme	CaCO3	949:953	arg1	order					895:899	helical order and homogeneous hybrid structures	887:933	order	895:899	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	7	5	theme	CaCO3	949:953	arg1	structures					924:933	helical order and homogeneous hybrid structures	887:933	structures	924:933	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	0	6	theme	Crustacean	122:131	arg1	Cuticles					133:140	Crustacean Cuticles	122:140	Crustacean Cuticles	122:140	Formation of Helically Structured Chitin/CaCO3 Hybrids through an Approach Inspired by the Biomineralization Processes of Crustacean Cuticles.
26192070	4	7	theme	colloidal	554:562	arg1	suspension					564:573	the colloidal suspension	550:573	the colloidal suspension of amorphous CaCO3 (ACC) at room temperature	550:618	The crystallization of CaCO3 is conducted by soaking the templates in the colloidal suspension of amorphous CaCO3 (ACC) at room temperature.
26192070	2	8	theme	ordered	343:349	arg1	templates					351:359	helically ordered templates	333:359	helically ordered templates	333:359	Liquid-crystalline chitin whiskers are used as helically ordered templates.
26192070	2	8	theme	ordered	343:349	arg1	whiskers					312:319	Liquid-crystalline chitin whiskers	286:319	Liquid-crystalline chitin whiskers	286:319	Liquid-crystalline chitin whiskers are used as helically ordered templates.
26192070	4	9	theme	amorphous	578:586	arg1	ACC					595:597	ACC	595:597	ACC	595:597	The crystallization of CaCO3 is conducted by soaking the templates in the colloidal suspension of amorphous CaCO3 (ACC) at room temperature.
26192070	4	9	theme	amorphous	578:586	arg1	CaCO3					588:592	amorphous CaCO3	578:592	amorphous CaCO3 (ACC)	578:598	The crystallization of CaCO3 is conducted by soaking the templates in the colloidal suspension of amorphous CaCO3 (ACC) at room temperature.
26192070	1	10	with	hybrids	156:162	arg1	structures					177:186	helical structures	169:186	helical structures	169:186	Chitin/CaCO3 hybrids with helical structures are formed through a biomineralization-inspired crystallization process under ambient conditions.
26192070	7	11	contain	possess	879:885	arg1	they					874:877	they	874:877	they	874:877	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	7	11	contain	possess	879:885	arg2	order					895:899	helical order and homogeneous hybrid structures	887:933	order	895:899	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	7	11	contain	possess	879:885	arg2	structures					924:933	helical order and homogeneous hybrid structures	887:933	structures	924:933	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	2	12	used	used	325:328	arg2	whiskers					312:319	Liquid-crystalline chitin whiskers	286:319	Liquid-crystalline chitin whiskers	286:319	Liquid-crystalline chitin whiskers are used as helically ordered templates.
26192070	2	12	used	used	325:328	arg2	templates					351:359	helically ordered templates	333:359	helically ordered templates	333:359	Liquid-crystalline chitin whiskers are used as helically ordered templates.
26192070	5	13	theme	crystallization	645:659	arg1	stage					636:640	the initial stage	624:640	the initial stage of crystallization	624:659	At the initial stage of crystallization, ACC particles are introduced inside the templates, and they crystallize to CaCO3 nanocrystals.
26192070	6	14	theme	polymer	768:774	arg1	networks					776:783	The acidic polymer networks	757:783	The acidic polymer networks	757:783	The acidic polymer networks induce CaCO3 crystallization.
26192070	7	15	dep	structure	976:984	arg1	the					972:974	the	972:974	the	972:974	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	6	16	theme	acidic	761:766	arg1	networks					776:783	The acidic polymer networks	757:783	The acidic polymer networks	757:783	The acidic polymer networks induce CaCO3 crystallization.
26192070	1	17	theme	biomineralization-inspired	209:234	arg1	process					252:258	a biomineralization-inspired crystallization process	207:258	a biomineralization-inspired crystallization process under ambient conditions	207:283	Chitin/CaCO3 hybrids with helical structures are formed through a biomineralization-inspired crystallization process under ambient conditions.
26192070	0	18	theme	Structured	23:32	arg1	Hybrids					47:53	Helically Structured Chitin/CaCO3 Hybrids	13:53	Helically Structured Chitin/CaCO3 Hybrids	13:53	Formation of Helically Structured Chitin/CaCO3 Hybrids through an Approach Inspired by the Biomineralization Processes of Crustacean Cuticles.
26192070	7	19	theme	homogeneous	905:915	arg1	structures					924:933	helical order and homogeneous hybrid structures	887:933	structures	924:933	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	5	20	theme	ACC	662:664	arg1	particles					666:674	ACC particles	662:674	ACC particles	662:674	At the initial stage of crystallization, ACC particles are introduced inside the templates, and they crystallize to CaCO3 nanocrystals.
26192070	7	21	theme	chitin	938:943	arg1	order					895:899	helical order and homogeneous hybrid structures	887:933	order	895:899	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	7	21	theme	chitin	938:943	arg1	structures					924:933	helical order and homogeneous hybrid structures	887:933	structures	924:933	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	1	22	theme	crystallization	236:250	arg1	process					252:258	a biomineralization-inspired crystallization process	207:258	a biomineralization-inspired crystallization process under ambient conditions	207:283	Chitin/CaCO3 hybrids with helical structures are formed through a biomineralization-inspired crystallization process under ambient conditions.
26192070	3	23	theme	chitin	462:467	arg1	templates					469:477	the chitin templates	458:477	the chitin templates	458:477	The liquid-crystalline structures are stabilized by acidic polymer networks which interact with the chitin templates.
26192070	7	24	theme	helical	887:893	arg1	order					895:899	helical order and homogeneous hybrid structures	887:933	order	895:899	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	0	25	theme	Cuticles	133:140	arg1	Processes					109:117	the Biomineralization Processes	87:117	the Biomineralization Processes of Crustacean Cuticles	87:140	Formation of Helically Structured Chitin/CaCO3 Hybrids through an Approach Inspired by the Biomineralization Processes of Crustacean Cuticles.
26192070	7	26	theme	hybrid	917:922	arg1	structures					924:933	helical order and homogeneous hybrid structures	887:933	structures	924:933	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	0	27	theme	Hybrids	47:53	arg1	Formation					0:8	Formation	0:8	Formation of Helically Structured Chitin/CaCO3 Hybrids through an Approach	0:73	Formation of Helically Structured Chitin/CaCO3 Hybrids through an Approach Inspired by the Biomineralization Processes of Crustacean Cuticles.
26192070	2	28	theme	chitin	305:310	arg1	templates					351:359	helically ordered templates	333:359	helically ordered templates	333:359	Liquid-crystalline chitin whiskers are used as helically ordered templates.
26192070	2	28	theme	chitin	305:310	arg1	whiskers					312:319	Liquid-crystalline chitin whiskers	286:319	Liquid-crystalline chitin whiskers	286:319	Liquid-crystalline chitin whiskers are used as helically ordered templates.
26192070	0	29	theme	Chitin/CaCO3	34:45	arg1	Hybrids					47:53	Helically Structured Chitin/CaCO3 Hybrids	13:53	Helically Structured Chitin/CaCO3 Hybrids	13:53	Formation of Helically Structured Chitin/CaCO3 Hybrids through an Approach Inspired by the Biomineralization Processes of Crustacean Cuticles.
26192070	4	30	theme	room	603:606	arg1	temperature					608:618	room temperature	603:618	room temperature	603:618	The crystallization of CaCO3 is conducted by soaking the templates in the colloidal suspension of amorphous CaCO3 (ACC) at room temperature.
26192070	4	31	from	temperature	608:618	arg1	suspension					564:573	the colloidal suspension	550:573	the colloidal suspension of amorphous CaCO3 (ACC) at room temperature	550:618	The crystallization of CaCO3 is conducted by soaking the templates in the colloidal suspension of amorphous CaCO3 (ACC) at room temperature.
26192070	2	32	theme	Liquid-crystalline	286:303	arg1	templates					351:359	helically ordered templates	333:359	helically ordered templates	333:359	Liquid-crystalline chitin whiskers are used as helically ordered templates.
26192070	2	32	theme	Liquid-crystalline	286:303	arg1	whiskers					312:319	Liquid-crystalline chitin whiskers	286:319	Liquid-crystalline chitin whiskers	286:319	Liquid-crystalline chitin whiskers are used as helically ordered templates.
26192070	1	33	theme	ambient	266:272	arg1	conditions					274:283	ambient conditions	266:283	ambient conditions	266:283	Chitin/CaCO3 hybrids with helical structures are formed through a biomineralization-inspired crystallization process under ambient conditions.
26192070	5	34	theme	initial	628:634	arg1	stage					636:640	the initial stage	624:640	the initial stage of crystallization	624:659	At the initial stage of crystallization, ACC particles are introduced inside the templates, and they crystallize to CaCO3 nanocrystals.
26192070	7	35	theme	crustaceans	1024:1034	arg1	exoskeleton					1009:1019	the exoskeleton	1005:1019	the exoskeleton of crustaceans	1005:1034	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	1	36	theme	Chitin/CaCO3	143:154	arg1	hybrids					156:162	Chitin/CaCO3 hybrids	143:162	Chitin/CaCO3 hybrids with helical structures	143:186	Chitin/CaCO3 hybrids with helical structures are formed through a biomineralization-inspired crystallization process under ambient conditions.
26192070	7	37	theme	exoskeleton	1009:1019	arg1	structure					976:984	structure	976:984	structure	976:984	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	7	37	theme	exoskeleton	1009:1019	arg1	composition					990:1000	composition	990:1000	composition	990:1000	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	7	38	theme	resultant	843:851	arg1	hybrids					853:859	the resultant hybrids	839:859	the resultant hybrids	839:859	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	3	39	theme	liquid-crystalline	366:383	arg1	structures					385:394	The liquid-crystalline structures	362:394	The liquid-crystalline structures	362:394	The liquid-crystalline structures are stabilized by acidic polymer networks which interact with the chitin templates.
26192070	7	40	theme	hybrids	853:859	arg1	characterization					819:834	The characterization	815:834	The characterization of the resultant hybrids	815:859	The characterization of the resultant hybrids reveals that they possess helical order and homogeneous hybrid structures of chitin and CaCO3 , which resemble the structure and composition of the exoskeleton of crustaceans.
26192070	3	41	theme	acidic	414:419	arg1	networks					429:436	acidic polymer networks	414:436	acidic polymer networks which interact with the chitin templates	414:477	The liquid-crystalline structures are stabilized by acidic polymer networks which interact with the chitin templates.
26192070	3	42	theme	polymer	421:427	arg1	networks					429:436	acidic polymer networks	414:436	acidic polymer networks which interact with the chitin templates	414:477	The liquid-crystalline structures are stabilized by acidic polymer networks which interact with the chitin templates.
26192070	0	43	theme	Helically	13:21	arg1	Hybrids					47:53	Helically Structured Chitin/CaCO3 Hybrids	13:53	Helically Structured Chitin/CaCO3 Hybrids	13:53	Formation of Helically Structured Chitin/CaCO3 Hybrids through an Approach Inspired by the Biomineralization Processes of Crustacean Cuticles.
26192070	1	44	theme	helical	169:175	arg1	structures					177:186	helical structures	169:186	helical structures	169:186	Chitin/CaCO3 hybrids with helical structures are formed through a biomineralization-inspired crystallization process under ambient conditions.
24299769	1	0	from	temperature	320:330	arg1	photopolymerization					272:290	ultraviolet (UV) photopolymerization	255:290	ultraviolet (UV) photopolymerization in aqueous solution at room temperature	255:330	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	1	0	from	temperature	320:330	arg1	irgacure					337:344	irgacure 651	337:348	irgacure 651	337:348	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	1	1	theme	cotton	188:193	arg1	crosslinker					218:228	maleylated cotton stalk cellulose (MCSC) crosslinker	177:228	maleylated cotton stalk cellulose (MCSC) crosslinker	177:228	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	1	2	theme	ultraviolet	255:265	arg1	photopolymerization					272:290	ultraviolet (UV) photopolymerization	255:290	ultraviolet (UV) photopolymerization in aqueous solution at room temperature	255:330	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	0	3	theme	polymer	100:106	arg1	characterization					24:39	characterization	24:39	characterization	24:39	Photopolymerisation and characterization of maleylatedcellulose-g-poly(acrylic acid) superabsorbent polymer.
24299769	0	3	theme	polymer	100:106	arg1	Photopolymerisation					0:18	Photopolymerisation	0:18	Photopolymerisation	0:18	Photopolymerisation and characterization of maleylatedcellulose-g-poly(acrylic acid) superabsorbent polymer.
24299769	3	4	theme	water	640:644	arg1	absorbency					646:655	the water absorbency	636:655	the water absorbency	636:655	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	2	5	dep	NMR	433:435	arg1	1					429:429	1	429:429	1	429:429	The resulting superabsorbent was characterized by FT-IR, (1)H NMR, SEM and TGA.
24299769	1	6	theme	stalk	195:199	arg1	crosslinker					218:228	maleylated cotton stalk cellulose (MCSC) crosslinker	177:228	maleylated cotton stalk cellulose (MCSC) crosslinker	177:228	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	3	7	theme	substitution	507:518	arg1	degree					497:502	degree	497:502	degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration	497:631	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	1	8	theme	UV	268:269	arg1	photopolymerization					272:290	ultraviolet (UV) photopolymerization	255:290	ultraviolet (UV) photopolymerization in aqueous solution at room temperature	255:330	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	4	9	theme	swelling	713:720	arg1	kinetics					722:729	The swelling kinetics	709:729	The swelling kinetics	709:729	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	4	9	theme	swelling	713:720	arg1	biodegradability					778:793	biodegradability	778:793	biodegradability	778:793	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	4	9	theme	swelling	713:720	arg1	capacity					765:772	water retention capacity	749:772	water retention capacity	749:772	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	4	9	theme	swelling	713:720	arg1	salt-resistance					732:746	salt-resistance	732:746	salt-resistance	732:746	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	3	10	theme	preparation	466:476	arg1	conditions					478:487	preparation conditions	466:487	preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration	466:631	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	10	theme	preparation	466:476	arg1	degree					497:502	degree	497:502	degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration	497:631	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	4	11	theme	superabsorbent	813:826	arg1	kinetics					722:729	The swelling kinetics	709:729	The swelling kinetics	709:729	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	4	11	theme	superabsorbent	813:826	arg1	biodegradability					778:793	biodegradability	778:793	biodegradability	778:793	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	4	11	theme	superabsorbent	813:826	arg1	capacity					765:772	water retention capacity	749:772	water retention capacity	749:772	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	4	11	theme	superabsorbent	813:826	arg1	salt-resistance					732:746	salt-resistance	732:746	salt-resistance	732:746	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	1	12	theme	cellulose	201:209	arg1	crosslinker					218:228	maleylated cotton stalk cellulose (MCSC) crosslinker	177:228	maleylated cotton stalk cellulose (MCSC) crosslinker	177:228	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	0	13	theme	acrylic	71:77	arg1	acid					79:82	acrylic acid	71:82	acrylic acid	71:82	Photopolymerisation and characterization of maleylatedcellulose-g-poly(acrylic acid) superabsorbent polymer.
24299769	3	14	theme	monomer	665:671	arg1	conversion					673:682	the monomer conversion	661:682	the monomer conversion	661:682	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	4	15	theme	MCSC-g-PAA	802:811	arg1	superabsorbent					813:826	the MCSC-g-PAA superabsorbent	798:826	the MCSC-g-PAA superabsorbent	798:826	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	3	16	from	conversion	673:682	arg1	graft					687:691	graft	687:691	graft	687:691	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	5	17	theme	obtained	870:877	arg1	superabsorbent					879:892	the obtained superabsorbent	866:892	the obtained superabsorbent	866:892	It was found that, the obtained superabsorbent have good swelling degree that greatly affected by its composition and preparation conditions.
24299769	1	18	from	photopolymerization	272:290	arg1	solution					303:310	aqueous solution	295:310	aqueous solution	295:310	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	1	19	theme	MCSC	212:215	arg1	crosslinker					218:228	maleylated cotton stalk cellulose (MCSC) crosslinker	177:228	maleylated cotton stalk cellulose (MCSC) crosslinker	177:228	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	2	20	theme	H	431:431	arg1	NMR					433:435	(1)H NMR	428:435	(1)H NMR	428:435	The resulting superabsorbent was characterized by FT-IR, (1)H NMR, SEM and TGA.
24299769	3	21	from	effects	455:461	arg1	conversion					673:682	the monomer conversion	661:682	the monomer conversion	661:682	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	21	from	effects	455:461	arg1	absorbency					646:655	the water absorbency	636:655	the water absorbency	636:655	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	1	22	theme	novel	111:115	arg1	photoinitiator					355:368	a photoinitiator	353:368	a photoinitiator	353:368	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	1	22	theme	novel	111:115	arg1	polymer					146:152	A novel biodegradable superabsorbent polymer	109:152	A novel biodegradable superabsorbent polymer	109:152	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	2	23	theme	resulting	375:383	arg1	superabsorbent					385:398	The resulting superabsorbent	371:398	The resulting superabsorbent	371:398	The resulting superabsorbent was characterized by FT-IR, (1)H NMR, SEM and TGA.
24299769	3	24	theme	maleylated	536:545	arg1	cellulose					560:568	maleylated cotton stalk cellulose	536:568	maleylated cotton stalk cellulose	536:568	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	1	25	theme	biodegradable	117:129	arg1	photoinitiator					355:368	a photoinitiator	353:368	a photoinitiator	353:368	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	1	25	theme	biodegradable	117:129	arg1	polymer					146:152	A novel biodegradable superabsorbent polymer	109:152	A novel biodegradable superabsorbent polymer	109:152	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	1	26	theme	aqueous	295:301	arg1	solution					303:310	aqueous solution	295:310	aqueous solution	295:310	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	5	27	contain	have	894:897	arg2	degree					913:918	good swelling degree that greatly affected by its composition and preparation conditions	899:986	good swelling degree that greatly affected by its composition and preparation conditions	899:986	It was found that, the obtained superabsorbent have good swelling degree that greatly affected by its composition and preparation conditions.
24299769	5	27	contain	have	894:897	arg1	superabsorbent					879:892	the obtained superabsorbent	866:892	the obtained superabsorbent	866:892	It was found that, the obtained superabsorbent have good swelling degree that greatly affected by its composition and preparation conditions.
24299769	1	28	theme	superabsorbent	131:144	arg1	photoinitiator					355:368	a photoinitiator	353:368	a photoinitiator	353:368	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	1	28	theme	superabsorbent	131:144	arg1	polymer					146:152	A novel biodegradable superabsorbent polymer	109:152	A novel biodegradable superabsorbent polymer	109:152	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	6	29	from	application	1116:1126	arg1	field					1143:1147	agriculture field	1131:1147	agriculture field	1131:1147	Owing to its considerable good water retention capacity, being economical and environment-friendly, it might be useful for its application in agriculture field.
24299769	6	30	theme	retention	1026:1034	arg1	capacity					1036:1043	its considerable good water retention capacity	998:1043	its considerable good water retention capacity	998:1043	Owing to its considerable good water retention capacity, being economical and environment-friendly, it might be useful for its application in agriculture field.
24299769	3	31	theme	cotton	547:552	arg1	cellulose					560:568	maleylated cotton stalk cellulose	536:568	maleylated cotton stalk cellulose	536:568	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	1	32	theme	acrylic	234:240	arg1	AA					248:249	AA	248:249	AA	248:249	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	1	32	theme	acrylic	234:240	arg1	acid					242:245	acrylic acid	234:245	acrylic acid (AA)	234:250	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	0	33	theme	maleylatedcellulose-g-poly	44:69	arg1	polymer					100:106	maleylatedcellulose-g-poly(acrylic acid) superabsorbent polymer	44:106	maleylatedcellulose-g-poly(acrylic acid) superabsorbent polymer	44:106	Photopolymerisation and characterization of maleylatedcellulose-g-poly(acrylic acid) superabsorbent polymer.
24299769	3	34	from	absorbency	646:655	arg1	graft					687:691	graft	687:691	graft	687:691	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	35	theme	stalk	554:558	arg1	cellulose					560:568	maleylated cotton stalk cellulose	536:568	maleylated cotton stalk cellulose	536:568	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	6	36	theme	water	1020:1024	arg1	capacity					1036:1043	its considerable good water retention capacity	998:1043	its considerable good water retention capacity	998:1043	Owing to its considerable good water retention capacity, being economical and environment-friendly, it might be useful for its application in agriculture field.
24299769	1	37	theme	room	315:318	arg1	temperature					320:330	room temperature	315:330	room temperature	315:330	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	3	38	theme	photoinitiator	585:598	arg1	amount					600:605	photoinitiator amount	585:605	photoinitiator amount	585:605	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	38	theme	photoinitiator	585:598	arg1	substitution					507:518	substitution	507:518	substitution (DS)	507:523	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	38	theme	photoinitiator	585:598	arg1	cellulose					560:568	maleylated cotton stalk cellulose	536:568	maleylated cotton stalk cellulose	536:568	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	1	39	from	irgacure	337:344	arg1	solution					303:310	aqueous solution	295:310	aqueous solution	295:310	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
24299769	0	40	dep	maleylatedcellulose-g-poly	44:69	arg1	acid					79:82	acrylic acid	71:82	acrylic acid	71:82	Photopolymerisation and characterization of maleylatedcellulose-g-poly(acrylic acid) superabsorbent polymer.
24299769	3	41	theme	conditions	478:487	arg1	effects					455:461	The effects	451:461	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft	451:691	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	4	42	theme	water	749:753	arg1	kinetics					722:729	The swelling kinetics	709:729	The swelling kinetics	709:729	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	4	42	theme	water	749:753	arg1	capacity					765:772	water retention capacity	749:772	water retention capacity	749:772	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	3	43	theme	exposed	571:577	arg1	substitution					507:518	substitution	507:518	substitution (DS)	507:523	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	43	theme	exposed	571:577	arg1	time					579:582	exposed time	571:582	exposed time	571:582	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	5	44	theme	good	899:902	arg1	degree					913:918	good swelling degree that greatly affected by its composition and preparation conditions	899:986	good swelling degree that greatly affected by its composition and preparation conditions	899:986	It was found that, the obtained superabsorbent have good swelling degree that greatly affected by its composition and preparation conditions.
24299769	6	45	theme	agriculture	1131:1141	arg1	field					1143:1147	agriculture field	1131:1147	agriculture field	1131:1147	Owing to its considerable good water retention capacity, being economical and environment-friendly, it might be useful for its application in agriculture field.
24299769	6	46	theme	good	1015:1018	arg1	capacity					1036:1043	its considerable good water retention capacity	998:1043	its considerable good water retention capacity	998:1043	Owing to its considerable good water retention capacity, being economical and environment-friendly, it might be useful for its application in agriculture field.
24299769	3	47	theme	monomer	611:617	arg1	substitution					507:518	substitution	507:518	substitution (DS)	507:523	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	47	theme	monomer	611:617	arg1	concentration					619:631	monomer concentration	611:631	monomer concentration	611:631	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	5	48	theme	swelling	904:911	arg1	degree					913:918	good swelling degree that greatly affected by its composition and preparation conditions	899:986	good swelling degree that greatly affected by its composition and preparation conditions	899:986	It was found that, the obtained superabsorbent have good swelling degree that greatly affected by its composition and preparation conditions.
24299769	6	49	theme	considerable	1002:1013	arg1	capacity					1036:1043	its considerable good water retention capacity	998:1043	its considerable good water retention capacity	998:1043	Owing to its considerable good water retention capacity, being economical and environment-friendly, it might be useful for its application in agriculture field.
24299769	0	50	theme	superabsorbent	85:98	arg1	polymer					100:106	maleylatedcellulose-g-poly(acrylic acid) superabsorbent polymer	44:106	maleylatedcellulose-g-poly(acrylic acid) superabsorbent polymer	44:106	Photopolymerisation and characterization of maleylatedcellulose-g-poly(acrylic acid) superabsorbent polymer.
24299769	3	51	theme	cellulose	560:568	arg1	amount					600:605	photoinitiator amount	585:605	photoinitiator amount	585:605	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	51	theme	cellulose	560:568	arg1	cellulose					560:568	maleylated cotton stalk cellulose	536:568	maleylated cotton stalk cellulose	536:568	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	51	theme	cellulose	560:568	arg1	amount					526:531	amount	526:531	amount of maleylated cotton stalk cellulose	526:568	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	51	theme	cellulose	560:568	arg1	time					579:582	exposed time	571:582	exposed time	571:582	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	51	theme	cellulose	560:568	arg1	substitution					507:518	substitution	507:518	substitution (DS)	507:523	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	3	51	theme	cellulose	560:568	arg1	concentration					619:631	monomer concentration	611:631	monomer concentration	611:631	The effects of preparation conditions such as degree of substitution (DS), amount of maleylated cotton stalk cellulose, exposed time, photoinitiator amount and monomer concentration on the water absorbency and the monomer conversion in graft were evaluated.
24299769	5	52	theme	preparation	965:975	arg1	conditions					977:986	preparation conditions	965:986	preparation conditions	965:986	It was found that, the obtained superabsorbent have good swelling degree that greatly affected by its composition and preparation conditions.
24299769	4	53	theme	retention	755:763	arg1	kinetics					722:729	The swelling kinetics	709:729	The swelling kinetics	709:729	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	4	53	theme	retention	755:763	arg1	capacity					765:772	water retention capacity	749:772	water retention capacity	749:772	The swelling kinetics, salt-resistance, water retention capacity and biodegradability of the MCSC-g-PAA superabsorbent were investigated.
24299769	1	54	theme	maleylated	177:186	arg1	crosslinker					218:228	maleylated cotton stalk cellulose (MCSC) crosslinker	177:228	maleylated cotton stalk cellulose (MCSC) crosslinker	177:228	A novel biodegradable superabsorbent polymer has been prepared from maleylated cotton stalk cellulose (MCSC) crosslinker and acrylic acid (AA) by ultraviolet (UV) photopolymerization in aqueous solution at room temperature, and irgacure 651 as a photoinitiator.
27913288	2	0	theme	females	374:380	arg1	organ					365:369	the copulatory organ	350:369	the copulatory organ of females of the bedbug	350:394	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	2	0	theme	females	374:380	arg1	lectularius					403:413	Cimex lectularius	397:413	Cimex lectularius	397:413	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	1	1	theme	used	248:251	arg1	characteristics					262:276	widely used spectral characteristics	241:276	widely used spectral characteristics	241:276	The decay time of the fluorescence of excited molecules, called fluorescence lifetime, can provide information about the cuticle composition additionally to widely used spectral characteristics.
27913288	0	2	theme	lectularius	71:81	arg1	cuticle					43:49	the cuticle	39:49	the cuticle of the bedbug Cimex lectularius	39:81	Autofluorescence lifetime variation in the cuticle of the bedbug Cimex lectularius.
27913288	1	3	theme	fluorescence	148:159	arg1	lifetime					161:168	fluorescence lifetime	148:168	fluorescence lifetime	148:168	The decay time of the fluorescence of excited molecules, called fluorescence lifetime, can provide information about the cuticle composition additionally to widely used spectral characteristics.
27913288	2	4	from	regions	339:345	arg1	organ					365:369	the copulatory organ	350:369	the copulatory organ of females of the bedbug	350:394	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	2	4	from	regions	339:345	arg1	lectularius					403:413	Cimex lectularius	397:413	Cimex lectularius	397:413	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	1	5	theme	spectral	253:260	arg1	characteristics					262:276	widely used spectral characteristics	241:276	widely used spectral characteristics	241:276	The decay time of the fluorescence of excited molecules, called fluorescence lifetime, can provide information about the cuticle composition additionally to widely used spectral characteristics.
27913288	3	6	theme	mean	555:558	arg1	τm					586:587	the mean autofluorescence lifetime τm	551:587	the mean autofluorescence lifetime τm (tau-m)	551:595	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	3	6	theme	mean	555:558	arg1	tau-m					590:594	tau-m	590:594	tau-m	590:594	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	4	7	theme	second	744:749	arg1	component					751:759	its second component	740:759	its second component	740:759	The mean lifetime, and particularly its second component, can be useful to distinguish resilin-rich from resilin-poor parts of the cuticle.
27913288	5	8	theme	autofluorescent	934:948	arg1	component					950:958	the main autofluorescent component	925:958	the main autofluorescent component observed in the resilin-poor areas in our study	925:1006	The few existing literature data suggest that chitin is unlikely responsible for the main autofluorescent component observed in the resilin-poor areas in our study and that melanin requires further scrutiny.
27913288	6	9	theme	multiphoton	1182:1192	arg1	excitation					1194:1203	multiphoton excitation	1182:1203	multiphoton excitation	1182:1203	Autofluorescence lifetime measurements can help to characterise properties of the arthropod cuticle, especially when coupled with multiphoton excitation to allow for deeper tissue penetration.
27913288	3	10	theme	autofluorescence	560:575	arg1	τm					586:587	the mean autofluorescence lifetime τm	551:587	the mean autofluorescence lifetime τm (tau-m)	551:595	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	3	10	theme	autofluorescence	560:575	arg1	tau-m					590:594	tau-m	590:594	tau-m	590:594	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	2	11	from	organ	365:369	arg1	lifetimes					308:316	autofluorescence lifetimes	291:316	autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius	291:413	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	2	12	theme	autofluorescence	291:306	arg1	lifetimes					308:316	autofluorescence lifetimes	291:316	autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius	291:413	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	5	13	theme	main	929:932	arg1	component					950:958	the main autofluorescent component	925:958	the main autofluorescent component observed in the resilin-poor areas in our study	925:1006	The few existing literature data suggest that chitin is unlikely responsible for the main autofluorescent component observed in the resilin-poor areas in our study and that melanin requires further scrutiny.
27913288	6	14	theme	lifetime	1069:1076	arg1	measurements					1078:1089	Autofluorescence lifetime measurements	1052:1089	Autofluorescence lifetime measurements	1052:1089	Autofluorescence lifetime measurements can help to characterise properties of the arthropod cuticle, especially when coupled with multiphoton excitation to allow for deeper tissue penetration.
27913288	5	15	theme	existing	852:859	arg1	data					872:875	The few existing literature data	844:875	The few existing literature data	844:875	The few existing literature data suggest that chitin is unlikely responsible for the main autofluorescent component observed in the resilin-poor areas in our study and that melanin requires further scrutiny.
27913288	4	16	theme	cuticle	835:841	arg1	parts					822:826	resilin-poor parts	809:826	resilin-poor parts of the cuticle	809:841	The mean lifetime, and particularly its second component, can be useful to distinguish resilin-rich from resilin-poor parts of the cuticle.
27913288	3	17	from	rich	495:498	arg1	resilin					503:509	resilin	503:509	resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns	503:701	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	2	18	theme	Cimex	397:401	arg1	lectularius					403:413	Cimex lectularius	397:413	Cimex lectularius	397:413	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	2	18	theme	Cimex	397:401	arg1	organ					365:369	the copulatory organ	350:369	the copulatory organ of females of the bedbug	350:394	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	2	19	theme	regions	339:345	arg1	lifetimes					308:316	autofluorescence lifetimes	291:316	autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius	291:413	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	3	20	from	resilin	503:509	arg1	rich					495:498	rich	495:498	rich	495:498	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	1	21	theme	decay	88:92	arg1	time					94:97	The decay time	84:97	The decay time	84:97	The decay time of the fluorescence of excited molecules, called fluorescence lifetime, can provide information about the cuticle composition additionally to widely used spectral characteristics.
27913288	2	22	theme	bedbug	389:394	arg1	females					374:380	females	374:380	females of the bedbug	374:394	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	2	23	theme	different	321:329	arg1	regions					339:345	different cuticle regions	321:345	different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius	321:413	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	2	24	theme	cuticle	331:337	arg1	regions					339:345	different cuticle regions	321:345	different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius	321:413	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	6	25	theme	deeper	1218:1223	arg1	penetration					1232:1242	deeper tissue penetration	1218:1242	deeper tissue penetration	1218:1242	Autofluorescence lifetime measurements can help to characterise properties of the arthropod cuticle, especially when coupled with multiphoton excitation to allow for deeper tissue penetration.
27913288	3	26	theme	τm	586:587	arg1	distribution					535:546	a longer bimodal distribution	518:546	a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m)	518:595	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	3	27	theme	bimodal	527:533	arg1	distribution					535:546	a longer bimodal distribution	518:546	a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m)	518:595	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	3	28	theme	two-photon	422:431	arg1	excitation					433:442	two-photon excitation	422:442	two-photon excitation at 720 nm	422:452	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	2	29	theme	copulatory	354:363	arg1	organ					365:369	the copulatory organ	350:369	the copulatory organ of females of the bedbug	350:394	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	2	29	theme	copulatory	354:363	arg1	lectularius					403:413	Cimex lectularius	397:413	Cimex lectularius	397:413	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	3	30	theme	unimodal	660:667	arg1	pattern					669:675	a unimodal pattern	658:675	a unimodal pattern	658:675	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	6	31	theme	Autofluorescence	1052:1067	arg1	measurements					1078:1089	Autofluorescence lifetime measurements	1052:1089	Autofluorescence lifetime measurements	1052:1089	Autofluorescence lifetime measurements can help to characterise properties of the arthropod cuticle, especially when coupled with multiphoton excitation to allow for deeper tissue penetration.
27913288	4	32	theme	resilin-poor	809:820	arg1	parts					822:826	resilin-poor parts	809:826	resilin-poor parts of the cuticle	809:841	The mean lifetime, and particularly its second component, can be useful to distinguish resilin-rich from resilin-poor parts of the cuticle.
27913288	1	33	theme	cuticle	205:211	arg1	composition					213:223	the cuticle composition	201:223	the cuticle composition additionally to widely used spectral characteristics	201:276	The decay time of the fluorescence of excited molecules, called fluorescence lifetime, can provide information about the cuticle composition additionally to widely used spectral characteristics.
27913288	5	34	theme	further	1034:1040	arg1	scrutiny					1042:1049	further scrutiny	1034:1049	further scrutiny	1034:1049	The few existing literature data suggest that chitin is unlikely responsible for the main autofluorescent component observed in the resilin-poor areas in our study and that melanin requires further scrutiny.
27913288	6	35	theme	arthropod	1134:1142	arg1	cuticle					1144:1150	the arthropod cuticle	1130:1150	the arthropod cuticle	1130:1150	Autofluorescence lifetime measurements can help to characterise properties of the arthropod cuticle, especially when coupled with multiphoton excitation to allow for deeper tissue penetration.
27913288	1	36	theme	fluorescence	106:117	arg1	time					94:97	The decay time	84:97	The decay time	84:97	The decay time of the fluorescence of excited molecules, called fluorescence lifetime, can provide information about the cuticle composition additionally to widely used spectral characteristics.
27913288	5	37	located	observed	960:967	arg1	areas					989:993	the resilin-poor areas	972:993	the resilin-poor areas in our study	972:1006	The few existing literature data suggest that chitin is unlikely responsible for the main autofluorescent component observed in the resilin-poor areas in our study and that melanin requires further scrutiny.
27913288	5	37	located	observed	960:967	arg2	component					950:958	the main autofluorescent component	925:958	the main autofluorescent component observed in the resilin-poor areas in our study	925:1006	The few existing literature data suggest that chitin is unlikely responsible for the main autofluorescent component observed in the resilin-poor areas in our study and that melanin requires further scrutiny.
27913288	3	38	theme	lifetime	577:584	arg1	τm					586:587	the mean autofluorescence lifetime τm	551:587	the mean autofluorescence lifetime τm (tau-m)	551:595	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	3	38	theme	lifetime	577:584	arg1	tau-m					590:594	tau-m	590:594	tau-m	590:594	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	6	39	theme	cuticle	1144:1150	arg1	properties					1116:1125	properties	1116:1125	properties of the arthropod cuticle	1116:1150	Autofluorescence lifetime measurements can help to characterise properties of the arthropod cuticle, especially when coupled with multiphoton excitation to allow for deeper tissue penetration.
27913288	3	40	theme	resilin-poor	629:640	arg1	sites					642:646	resilin-poor sites	629:646	resilin-poor sites	629:646	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	1	41	theme	excited	122:128	arg1	molecules					130:138	excited molecules	122:138	excited molecules	122:138	The decay time of the fluorescence of excited molecules, called fluorescence lifetime, can provide information about the cuticle composition additionally to widely used spectral characteristics.
27913288	2	42	from	lifetimes	308:316	arg1	organ					365:369	the copulatory organ	350:369	the copulatory organ of females of the bedbug	350:394	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	2	42	from	lifetimes	308:316	arg1	lectularius					403:413	Cimex lectularius	397:413	Cimex lectularius	397:413	We compared autofluorescence lifetimes of different cuticle regions in the copulatory organ of females of the bedbug, Cimex lectularius.
27913288	0	43	theme	Cimex	65:69	arg1	lectularius					71:81	the bedbug Cimex lectularius	54:81	the bedbug Cimex lectularius	54:81	Autofluorescence lifetime variation in the cuticle of the bedbug Cimex lectularius.
27913288	5	44	theme	few	848:850	arg1	data					872:875	The few existing literature data	844:875	The few existing literature data	844:875	The few existing literature data suggest that chitin is unlikely responsible for the main autofluorescent component observed in the resilin-poor areas in our study and that melanin requires further scrutiny.
27913288	3	45	from	720 nm	447:452	arg1	excitation					433:442	two-photon excitation	422:442	two-photon excitation at 720 nm	422:452	After two-photon excitation at 720 nm, regions recently characterised as being rich in resilin showed a longer bimodal distribution of the mean autofluorescence lifetime τm (tau-m) at 0.4 ns and 1.0-1.5 ns, while resilin-poor sites exhibited a unimodal pattern with a peak around 0.8 ns.
27913288	1	46	theme	molecules	130:138	arg1	fluorescence					106:117	the fluorescence	102:117	the fluorescence of excited molecules	102:138	The decay time of the fluorescence of excited molecules, called fluorescence lifetime, can provide information about the cuticle composition additionally to widely used spectral characteristics.
27913288	5	47	theme	literature	861:870	arg1	data					872:875	The few existing literature data	844:875	The few existing literature data	844:875	The few existing literature data suggest that chitin is unlikely responsible for the main autofluorescent component observed in the resilin-poor areas in our study and that melanin requires further scrutiny.
27913288	0	48	theme	bedbug	58:63	arg1	lectularius					71:81	the bedbug Cimex lectularius	54:81	the bedbug Cimex lectularius	54:81	Autofluorescence lifetime variation in the cuticle of the bedbug Cimex lectularius.
27913288	5	49	theme	resilin-poor	976:987	arg1	areas					989:993	the resilin-poor areas	972:993	the resilin-poor areas in our study	972:1006	The few existing literature data suggest that chitin is unlikely responsible for the main autofluorescent component observed in the resilin-poor areas in our study and that melanin requires further scrutiny.
27913288	6	50	theme	tissue	1225:1230	arg1	penetration					1232:1242	deeper tissue penetration	1218:1242	deeper tissue penetration	1218:1242	Autofluorescence lifetime measurements can help to characterise properties of the arthropod cuticle, especially when coupled with multiphoton excitation to allow for deeper tissue penetration.
27913288	5	51	from	areas	989:993	arg1	study					1002:1006	our study	998:1006	our study	998:1006	The few existing literature data suggest that chitin is unlikely responsible for the main autofluorescent component observed in the resilin-poor areas in our study and that melanin requires further scrutiny.
27913288	4	52	theme	mean	708:711	arg1	lifetime					713:720	The mean lifetime	704:720	The mean lifetime	704:720	The mean lifetime, and particularly its second component, can be useful to distinguish resilin-rich from resilin-poor parts of the cuticle.
27913288	4	52	theme	mean	708:711	arg1	useful					769:774	useful	769:774	useful	769:774	The mean lifetime, and particularly its second component, can be useful to distinguish resilin-rich from resilin-poor parts of the cuticle.
28028582	6	0	theme	grain	1090:1094	arg1	quality					1096:1102	grain quality	1090:1102	grain quality for food, feed, and fuel products	1090:1136	Coordinated association and linkage mapping revealed several robust QTLs that make prime targets to improve grain quality for food, feed, and fuel products.
28028582	2	1	theme	products	332:339	arg1	range					315:319	a wide range	308:319	a wide range of end-use products made from cereal grains	308:363	There is a wide range of end-use products made from cereal grains, and these products often demand different grain characteristics.
28028582	3	2	theme	phenotypic	525:534	arg1	variation					536:544	high phenotypic variation	520:544	high phenotypic variation for traits influencing grain quality	520:581	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	11	3	theme	future	1942:1947	arg1	studies					1949:1955	future studies	1942:1955	future studies for gene validation	1942:1975	Collectively, these genetic regions offer excellent opportunities to manipulate grain composition and set up future studies for gene validation.
28028582	1	4	theme	genetic	263:269	arg1	markers					271:277	high-density genetic markers	250:277	high-density genetic markers in linkage mapping	250:296	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	5	5	dep	environments	826:837	arg1	environments					826:837	two environments	822:837	two environments (SC Coastal Plain and Central TX)	822:871	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	5	dep	environments	826:837	arg1	Plain					851:855	SC Coastal Plain	840:855	SC Coastal Plain	840:855	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	5	dep	environments	826:837	arg1	TX					869:870	Central TX	861:870	Central TX	861:870	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	1	6	from	mapping	290:296	arg1	use					243:245	the use	239:245	the use of high-density genetic markers in linkage mapping	239:296	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	5	7	theme	Coastal	843:849	arg1	environments					826:837	two environments	822:837	two environments (SC Coastal Plain and Central TX)	822:871	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	7	theme	Coastal	843:849	arg1	Plain					851:855	SC Coastal Plain	840:855	SC Coastal Plain	840:855	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	8	theme	major	893:897	arg1	starch					930:935	starch	930:935	starch	930:935	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	8	theme	major	893:897	arg1	fat					959:961	crude fat	953:961	crude fat	953:961	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	8	theme	major	893:897	arg1	traits					913:918	five major grain quality traits	888:918	five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy	888:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	8	theme	major	893:897	arg1	protein					944:950	crude protein	938:950	crude protein	938:950	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	8	theme	major	893:897	arg1	energy					974:979	gross energy	968:979	gross energy	968:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	8	theme	major	893:897	arg1	amylose					921:927	amylose	921:927	amylose	921:927	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	9	9	theme	critical	1620:1627	arg1	locus					1611:1615	the DGAT1 locus	1601:1615	the DGAT1 locus	1601:1615	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	9	9	theme	critical	1620:1627	arg1	gene					1629:1632	a critical gene	1618:1632	a critical gene involved in maize lipid biosynthesis	1618:1669	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	7	10	from	waxy	1263:1266	arg1	12 kb					1252:1256	12 kb	1252:1256	12 kb	1252:1256	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
28028582	7	10	from	waxy	1263:1266	arg1	marker					1201:1206	the marker	1197:1206	the marker with greatest significance	1197:1233	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
28028582	6	11	theme	fuel	1124:1127	arg1	products					1129:1136	fuel products	1124:1136	fuel products	1124:1136	Coordinated association and linkage mapping revealed several robust QTLs that make prime targets to improve grain quality for food, feed, and fuel products.
28028582	3	12	dep	L.	500:501	arg1	Moench					504:509	Moench	504:509	Moench	504:509	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	11	13	theme	gene	1961:1964	arg1	validation					1966:1975	gene validation	1961:1975	gene validation	1961:1975	Collectively, these genetic regions offer excellent opportunities to manipulate grain composition and set up future studies for gene validation.
28028582	8	14	theme	RIL	1407:1409	arg1	populations					1412:1422	recombinant inbred line (RIL) populations	1382:1422	recombinant inbred line (RIL) populations	1382:1422	This suggests higher resolution mapping in recombinant inbred line (RIL) populations can be obtained when genotyped at a high marker density.
28028582	4	15	theme	plant	657:661	arg1	breeders					663:670	plant breeders	657:670	plant breeders	657:670	Identifying genetic variants underlying this phenotypic variation allows plant breeders to develop genotypes with grain attributes optimized for their intended usage.
28028582	10	16	theme	RIL	1732:1734	arg1	populations					1736:1746	both RIL populations	1727:1746	both RIL populations	1727:1746	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	9	17	theme	maize	1646:1650	arg1	biosynthesis					1658:1669	maize lipid biosynthesis	1646:1669	maize lipid biosynthesis	1646:1669	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	11	18	theme	excellent	1875:1883	arg1	opportunities					1885:1897	excellent opportunities	1875:1897	excellent opportunities	1875:1897	Collectively, these genetic regions offer excellent opportunities to manipulate grain composition and set up future studies for gene validation.
28028582	10	19	from	QTL	1680:1682	arg1	chromosome					1687:1696	chromosome 1	1687:1698	chromosome 1	1687:1698	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	10	20	theme	grain	1761:1765	arg1	starch					1792:1797	starch	1792:1797	starch	1792:1797	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	10	20	theme	grain	1761:1765	arg1	protein					1806:1812	crude protein	1800:1812	crude protein	1800:1812	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	10	20	theme	grain	1761:1765	arg1	traits					1775:1780	multiple grain quality traits	1752:1780	multiple grain quality traits including starch, crude protein, and gross energy	1752:1830	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	10	20	theme	grain	1761:1765	arg1	energy					1825:1830	gross energy	1819:1830	gross energy	1819:1830	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	7	21	with	marker	1201:1206	arg1	significance					1222:1233	greatest significance	1213:1233	greatest significance	1213:1233	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
28028582	2	22	theme	different	398:406	arg1	characteristics					414:428	different grain characteristics	398:428	different grain characteristics	398:428	There is a wide range of end-use products made from cereal grains, and these products often demand different grain characteristics.
28028582	4	23	theme	grain	698:702	arg1	attributes					704:713	grain attributes	698:713	grain attributes optimized for their intended usage	698:748	Identifying genetic variants underlying this phenotypic variation allows plant breeders to develop genotypes with grain attributes optimized for their intended usage.
28028582	11	24	theme	grain	1913:1917	arg1	composition					1919:1929	grain composition	1913:1929	grain composition	1913:1929	Collectively, these genetic regions offer excellent opportunities to manipulate grain composition and set up future studies for gene validation.
28028582	5	25	theme	quality	905:911	arg1	starch					930:935	starch	930:935	starch	930:935	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	25	theme	quality	905:911	arg1	fat					959:961	crude fat	953:961	crude fat	953:961	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	25	theme	quality	905:911	arg1	traits					913:918	five major grain quality traits	888:918	five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy	888:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	25	theme	quality	905:911	arg1	protein					944:950	crude protein	938:950	crude protein	938:950	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	25	theme	quality	905:911	arg1	energy					974:979	gross energy	968:979	gross energy	968:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	25	theme	quality	905:911	arg1	amylose					921:927	amylose	921:927	amylose	921:927	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	9	26	theme	major	1485:1489	arg1	QTL					1491:1493	The major QTL	1481:1493	The major QTL	1481:1493	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	6	27	theme	Coordinated	982:992	arg1	mapping					1018:1024	Coordinated association and linkage mapping	982:1024	Coordinated association and linkage mapping	982:1024	Coordinated association and linkage mapping revealed several robust QTLs that make prime targets to improve grain quality for food, feed, and fuel products.
28028582	8	28	theme	high	1460:1463	arg1	density					1472:1478	a high marker density	1458:1478	a high marker density	1458:1478	This suggests higher resolution mapping in recombinant inbred line (RIL) populations can be obtained when genotyped at a high marker density.
28028582	5	29	theme	Multiple	751:758	arg1	populations					776:786	Multiple sorghum mapping populations	751:786	Multiple sorghum mapping populations	751:786	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	30	theme	mapping	768:774	arg1	populations					776:786	Multiple sorghum mapping populations	751:786	Multiple sorghum mapping populations	751:786	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	31	theme	gross	968:972	arg1	traits					913:918	five major grain quality traits	888:918	five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy	888:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	31	theme	gross	968:972	arg1	energy					974:979	gross energy	968:979	gross energy	968:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	32	dep	traits	913:918	arg1	starch					930:935	starch	930:935	starch	930:935	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	32	dep	traits	913:918	arg1	fat					959:961	crude fat	953:961	crude fat	953:961	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	32	dep	traits	913:918	arg1	traits					913:918	five major grain quality traits	888:918	five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy	888:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	32	dep	traits	913:918	arg1	protein					944:950	crude protein	938:950	crude protein	938:950	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	32	dep	traits	913:918	arg1	energy					974:979	gross energy	968:979	gross energy	968:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	32	dep	traits	913:918	arg1	amylose					921:927	amylose	921:927	amylose	921:927	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	0	33	theme	Genetic	0:6	arg1	dissection					8:17	Genetic dissection	0:17	Genetic dissection of sorghum grain quality	0:42	Genetic dissection of sorghum grain quality traits using diverse and segregating populations.
28028582	1	34	theme	markers	271:277	arg1	use					243:245	the use	239:245	the use of high-density genetic markers in linkage mapping	239:296	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	1	35	theme	Coordinated	94:104	arg1	association					106:116	Coordinated association and linkage mapping	94:136	association	106:116	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	7	36	theme	primary	1278:1284	arg1	gene					1286:1289	the primary gene	1274:1289	the primary gene regulating amylose production in cereal grains	1274:1336	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
28028582	7	36	theme	primary	1278:1284	arg1	waxy					1263:1266	waxy	1263:1266	waxy (Wx)	1263:1271	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
28028582	7	37	theme	amylose	1302:1308	arg1	production					1310:1319	amylose production	1302:1319	amylose production in cereal grains	1302:1336	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
28028582	3	38	theme	crop	451:454	arg1	species					456:462	cereal crop species	444:462	cereal crop species including sorghum [Sorghum bicolor (L.) Moench]	444:510	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	1	39	theme	linkage	282:288	arg1	mapping					290:296	linkage mapping	282:296	linkage mapping	282:296	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	5	40	theme	crude	953:957	arg1	traits					913:918	five major grain quality traits	888:918	five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy	888:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	40	theme	crude	953:957	arg1	fat					959:961	crude fat	953:961	crude fat	953:961	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	1	41	theme	grain	152:156	arg1	QTLs					166:169	25 grain quality QTLs	149:169	25 grain quality QTLs	149:169	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	7	42	theme	QTL	1160:1162	arg1	interval					1164:1171	the amylose QTL interval	1148:1171	the amylose QTL interval	1148:1171	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
28028582	8	43	theme	resolution	1360:1369	arg1	mapping					1371:1377	higher resolution mapping	1353:1377	higher resolution mapping in recombinant inbred line (RIL) populations	1353:1422	This suggests higher resolution mapping in recombinant inbred line (RIL) populations can be obtained when genotyped at a high marker density.
28028582	0	44	theme	grain	30:34	arg1	quality					36:42	sorghum grain quality	22:42	sorghum grain quality	22:42	Genetic dissection of sorghum grain quality traits using diverse and segregating populations.
28028582	6	45	theme	several	1035:1041	arg1	QTLs					1050:1053	several robust QTLs	1035:1053	several robust QTLs that make prime targets to improve grain quality for food, feed, and fuel products	1035:1136	Coordinated association and linkage mapping revealed several robust QTLs that make prime targets to improve grain quality for food, feed, and fuel products.
28028582	2	46	theme	end-use	324:330	arg1	products					332:339	end-use products	324:339	end-use products made from cereal grains	324:363	There is a wide range of end-use products made from cereal grains, and these products often demand different grain characteristics.
28028582	11	47	theme	genetic	1853:1859	arg1	regions					1861:1867	these genetic regions	1847:1867	these genetic regions	1847:1867	Collectively, these genetic regions offer excellent opportunities to manipulate grain composition and set up future studies for gene validation.
28028582	8	48	theme	inbred	1394:1399	arg1	populations					1412:1422	recombinant inbred line (RIL) populations	1382:1422	recombinant inbred line (RIL) populations	1382:1422	This suggests higher resolution mapping in recombinant inbred line (RIL) populations can be obtained when genotyped at a high marker density.
28028582	1	49	theme	Wx	221:222	arg1	locus					224:228	the Wx locus	217:228	the Wx locus	217:228	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	9	50	theme	grain	1558:1562	arg1	panel					1582:1586	grain sorghum diversity panel	1558:1586	grain sorghum diversity panel	1558:1586	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	1	51	theme	quality	158:164	arg1	QTLs					166:169	25 grain quality QTLs	149:169	25 grain quality QTLs	149:169	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	4	52	theme	genetic	596:602	arg1	variants					604:611	genetic variants	596:611	genetic variants underlying this phenotypic variation	596:648	Identifying genetic variants underlying this phenotypic variation allows plant breeders to develop genotypes with grain attributes optimized for their intended usage.
28028582	10	53	theme	crude	1800:1804	arg1	protein					1806:1812	crude protein	1800:1812	crude protein	1800:1812	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	2	54	theme	cereal	351:356	arg1	grains					358:363	cereal grains	351:363	cereal grains	351:363	There is a wide range of end-use products made from cereal grains, and these products often demand different grain characteristics.
28028582	9	55	theme	diversity	1572:1580	arg1	panel					1582:1586	grain sorghum diversity panel	1558:1586	grain sorghum diversity panel	1558:1586	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	10	56	theme	gross	1819:1823	arg1	energy					1825:1830	gross energy	1819:1830	gross energy	1819:1830	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	3	57	theme	high	520:523	arg1	variation					536:544	high phenotypic variation	520:544	high phenotypic variation for traits influencing grain quality	520:581	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	1	58	theme	high-density	250:261	arg1	markers					271:277	high-density genetic markers	250:277	high-density genetic markers in linkage mapping	250:296	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	3	59	theme	[Sorghum	482:489	arg1	L.					500:501	L.	500:501	L.	500:501	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	3	59	theme	[Sorghum	482:489	arg1	bicolor					491:497	sorghum [Sorghum bicolor	474:497	sorghum [Sorghum bicolor (L.) Moench]	474:510	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	8	60	from	mapping	1371:1377	arg1	populations					1412:1422	recombinant inbred line (RIL) populations	1382:1422	recombinant inbred line (RIL) populations	1382:1422	This suggests higher resolution mapping in recombinant inbred line (RIL) populations can be obtained when genotyped at a high marker density.
28028582	1	61	theme	multiple	174:181	arg1	environments					183:194	multiple environments	174:194	multiple environments	174:194	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	1	62	from	markers	271:277	arg1	mapping					290:296	linkage mapping	282:296	linkage mapping	282:296	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	5	63	theme	Central	861:867	arg1	environments					826:837	two environments	822:837	two environments (SC Coastal Plain and Central TX)	822:871	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	63	theme	Central	861:867	arg1	TX					869:870	Central TX	861:870	Central TX	861:870	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	4	64	theme	phenotypic	629:638	arg1	variation					640:648	this phenotypic variation	624:648	this phenotypic variation	624:648	Identifying genetic variants underlying this phenotypic variation allows plant breeders to develop genotypes with grain attributes optimized for their intended usage.
28028582	1	65	from	use	243:245	arg1	mapping					290:296	linkage mapping	282:296	linkage mapping	282:296	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	3	66	theme	grain	569:573	arg1	quality					575:581	grain quality	569:581	grain quality	569:581	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	1	67	theme	linkage	122:128	arg1	mapping					130:136	Coordinated association and linkage mapping	94:136	mapping	130:136	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	9	68	theme	lipid	1652:1656	arg1	biosynthesis					1658:1669	maize lipid biosynthesis	1646:1669	maize lipid biosynthesis	1646:1669	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	10	69	theme	quality	1767:1773	arg1	starch					1792:1797	starch	1792:1797	starch	1792:1797	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	10	69	theme	quality	1767:1773	arg1	protein					1806:1812	crude protein	1800:1812	crude protein	1800:1812	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	10	69	theme	quality	1767:1773	arg1	traits					1775:1780	multiple grain quality traits	1752:1780	multiple grain quality traits including starch, crude protein, and gross energy	1752:1830	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	10	69	theme	quality	1767:1773	arg1	energy					1825:1830	gross energy	1819:1830	gross energy	1819:1830	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	7	70	theme	cereal	1324:1329	arg1	grains					1331:1336	cereal grains	1324:1336	cereal grains	1324:1336	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
28028582	4	71	theme	intended	735:742	arg1	usage					744:748	their intended usage	729:748	their intended usage	729:748	Identifying genetic variants underlying this phenotypic variation allows plant breeders to develop genotypes with grain attributes optimized for their intended usage.
28028582	10	72	theme	multiple	1752:1759	arg1	starch					1792:1797	starch	1792:1797	starch	1792:1797	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	10	72	theme	multiple	1752:1759	arg1	protein					1806:1812	crude protein	1800:1812	crude protein	1800:1812	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	10	72	theme	multiple	1752:1759	arg1	traits					1775:1780	multiple grain quality traits	1752:1780	multiple grain quality traits including starch, crude protein, and gross energy	1752:1830	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	10	72	theme	multiple	1752:1759	arg1	energy					1825:1830	gross energy	1819:1830	gross energy	1819:1830	Another QTL on chromosome 1 was consistently mapped in both RIL populations for multiple grain quality traits including starch, crude protein, and gross energy.
28028582	1	73	theme	fine	201:204	arg1	mapping					206:212	fine mapping	201:212	fine mapping of the Wx locus	201:228	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	5	74	theme	grain	899:903	arg1	starch					930:935	starch	930:935	starch	930:935	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	74	theme	grain	899:903	arg1	fat					959:961	crude fat	953:961	crude fat	953:961	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	74	theme	grain	899:903	arg1	traits					913:918	five major grain quality traits	888:918	five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy	888:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	74	theme	grain	899:903	arg1	protein					944:950	crude protein	938:950	crude protein	938:950	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	74	theme	grain	899:903	arg1	energy					974:979	gross energy	968:979	gross energy	968:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	74	theme	grain	899:903	arg1	amylose					921:927	amylose	921:927	amylose	921:927	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	6	75	theme	linkage	1010:1016	arg1	mapping					1018:1024	Coordinated association and linkage mapping	982:1024	Coordinated association and linkage mapping	982:1024	Coordinated association and linkage mapping revealed several robust QTLs that make prime targets to improve grain quality for food, feed, and fuel products.
28028582	9	76	theme	DGAT1	1605:1609	arg1	locus					1611:1615	the DGAT1 locus	1601:1615	the DGAT1 locus	1601:1615	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	9	76	theme	DGAT1	1605:1609	arg1	gene					1629:1632	a critical gene	1618:1632	a critical gene involved in maize lipid biosynthesis	1618:1669	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	6	77	theme	association	994:1004	arg1	mapping					1018:1024	Coordinated association and linkage mapping	982:1024	Coordinated association and linkage mapping	982:1024	Coordinated association and linkage mapping revealed several robust QTLs that make prime targets to improve grain quality for food, feed, and fuel products.
28028582	8	78	theme	marker	1465:1470	arg1	density					1472:1478	a high marker density	1458:1478	a high marker density	1458:1478	This suggests higher resolution mapping in recombinant inbred line (RIL) populations can be obtained when genotyped at a high marker density.
28028582	7	79	theme	many	1181:1184	arg1	megabases					1186:1194	many megabases	1181:1194	many megabases	1181:1194	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
28028582	3	80	theme	sorghum	474:480	arg1	L.					500:501	L.	500:501	L.	500:501	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	3	80	theme	sorghum	474:480	arg1	bicolor					491:497	sorghum [Sorghum bicolor	474:497	sorghum [Sorghum bicolor (L.) Moench]	474:510	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	5	81	theme	sorghum	760:766	arg1	populations					776:786	Multiple sorghum mapping populations	751:786	Multiple sorghum mapping populations	751:786	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	9	82	theme	crude	1499:1503	arg1	content					1509:1515	crude fat content	1499:1515	crude fat content	1499:1515	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	7	83	from	production	1310:1319	arg1	grains					1331:1336	cereal grains	1324:1336	cereal grains	1324:1336	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
28028582	5	84	theme	SC	840:841	arg1	environments					826:837	two environments	822:837	two environments (SC Coastal Plain and Central TX)	822:871	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	84	theme	SC	840:841	arg1	Plain					851:855	SC Coastal Plain	840:855	SC Coastal Plain	840:855	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	85	theme	crude	938:942	arg1	traits					913:918	five major grain quality traits	888:918	five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy	888:979	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	5	85	theme	crude	938:942	arg1	protein					944:950	crude protein	938:950	crude protein	938:950	Multiple sorghum mapping populations were rigorously phenotyped across two environments (SC Coastal Plain and Central TX) in 2 years for five major grain quality traits: amylose, starch, crude protein, crude fat, and gross energy.
28028582	6	86	theme	prime	1065:1069	arg1	targets					1071:1077	prime targets	1065:1077	prime targets	1065:1077	Coordinated association and linkage mapping revealed several robust QTLs that make prime targets to improve grain quality for food, feed, and fuel products.
28028582	0	87	theme	sorghum	22:28	arg1	quality					36:42	sorghum grain quality	22:42	sorghum grain quality	22:42	Genetic dissection of sorghum grain quality traits using diverse and segregating populations.
28028582	2	88	theme	grain	408:412	arg1	characteristics					414:428	different grain characteristics	398:428	different grain characteristics	398:428	There is a wide range of end-use products made from cereal grains, and these products often demand different grain characteristics.
28028582	4	89	with	genotypes	683:691	arg1	attributes					704:713	grain attributes	698:713	grain attributes optimized for their intended usage	698:748	Identifying genetic variants underlying this phenotypic variation allows plant breeders to develop genotypes with grain attributes optimized for their intended usage.
28028582	0	90	theme	quality	36:42	arg1	dissection					8:17	Genetic dissection	0:17	Genetic dissection of sorghum grain quality	0:42	Genetic dissection of sorghum grain quality traits using diverse and segregating populations.
28028582	6	91	theme	robust	1043:1048	arg1	QTLs					1050:1053	several robust QTLs	1035:1053	several robust QTLs that make prime targets to improve grain quality for food, feed, and fuel products	1035:1136	Coordinated association and linkage mapping revealed several robust QTLs that make prime targets to improve grain quality for food, feed, and fuel products.
28028582	3	92	contain	contain	512:518	arg2	variation					536:544	high phenotypic variation	520:544	high phenotypic variation for traits influencing grain quality	520:581	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	3	92	contain	contain	512:518	arg1	species					456:462	cereal crop species	444:462	cereal crop species including sorghum [Sorghum bicolor (L.) Moench]	444:510	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	8	93	theme	higher	1353:1358	arg1	mapping					1371:1377	higher resolution mapping	1353:1377	higher resolution mapping in recombinant inbred line (RIL) populations	1353:1422	This suggests higher resolution mapping in recombinant inbred line (RIL) populations can be obtained when genotyped at a high marker density.
28028582	3	94	theme	cereal	444:449	arg1	species					456:462	cereal crop species	444:462	cereal crop species including sorghum [Sorghum bicolor (L.) Moench]	444:510	Fortunately, cereal crop species including sorghum [Sorghum bicolor (L.) Moench] contain high phenotypic variation for traits influencing grain quality.
28028582	9	95	theme	RIL	1539:1541	arg1	population					1543:1552	a RIL population	1537:1552	a RIL population	1537:1552	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	9	96	theme	fat	1505:1507	arg1	content					1509:1515	crude fat content	1499:1515	crude fat content	1499:1515	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	2	97	theme	wide	310:313	arg1	range					315:319	a wide range	308:319	a wide range of end-use products made from cereal grains	308:363	There is a wide range of end-use products made from cereal grains, and these products often demand different grain characteristics.
28028582	1	98	theme	locus	224:228	arg1	mapping					206:212	fine mapping	201:212	fine mapping of the Wx locus	201:228	Coordinated association and linkage mapping identified 25 grain quality QTLs in multiple environments, and fine mapping of the Wx locus supports the use of high-density genetic markers in linkage mapping.
28028582	9	99	theme	sorghum	1564:1570	arg1	panel					1582:1586	grain sorghum diversity panel	1558:1586	grain sorghum diversity panel	1558:1586	The major QTL for crude fat content, identified in both a RIL population and grain sorghum diversity panel, encompassed the DGAT1 locus, a critical gene involved in maize lipid biosynthesis.
28028582	8	100	theme	line	1401:1404	arg1	populations					1412:1422	recombinant inbred line (RIL) populations	1382:1422	recombinant inbred line (RIL) populations	1382:1422	This suggests higher resolution mapping in recombinant inbred line (RIL) populations can be obtained when genotyped at a high marker density.
28028582	7	101	theme	amylose	1152:1158	arg1	interval					1164:1171	the amylose QTL interval	1148:1171	the amylose QTL interval	1148:1171	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
28028582	7	102	theme	greatest	1213:1220	arg1	significance					1222:1233	greatest significance	1213:1233	greatest significance	1213:1233	Although the amylose QTL interval spanned many megabases, the marker with greatest significance was located just 12 kb from waxy (Wx), the primary gene regulating amylose production in cereal grains.
26886338	5	0	theme	Mechanical	520:529	arg1	viscous					565:571	viscous	565:571	viscous	565:571	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	0	theme	Mechanical	520:529	arg1	viscoelasticity					578:592	viscoelasticity	578:592	viscoelasticity	578:592	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	0	theme	Mechanical	520:529	arg1	variables					646:654	response variables	637:654	response variables	637:654	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	0	theme	Mechanical	520:529	arg1	resistance					531:540	Mechanical resistance	520:540	Mechanical resistance	520:540	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	0	theme	Mechanical	520:529	arg1	propensity					553:562	hydration propensity	543:562	hydration propensity	543:562	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	1	1	theme	Development	147:157	arg1	Design					116:121	Design	116:121	Design of Experiments-Assisted Development	116:157	I. Design of Experiments-Assisted Development.
26886338	6	2	theme	response	741:748	arg1	variable					750:757	each response variable	736:757	each response variable	736:757	The superimposition of the contour plots, calculated by the best fit model for each response variable, permitted to individuate a region of the factor space where the dressing of optimized quantitative composition was chosen.
26886338	8	3	from	properties	1130:1139	arg1	line					1217:1220	human fibroblast cell line	1195:1220	human fibroblast cell line	1195:1220	The optimized formulation was characterized by optimal mechanical properties and by cell proliferation and antioxidant activity on human fibroblast cell line.
26886338	8	4	theme	cell	1148:1151	arg1	proliferation					1153:1165	cell proliferation	1148:1165	cell proliferation	1148:1165	The optimized formulation was characterized by optimal mechanical properties and by cell proliferation and antioxidant activity on human fibroblast cell line.
26886338	4	5	theme	simplex	495:501	arg1	design					512:517	a simplex centroid design	493:517	a simplex centroid design	493:517	Dressing development was assisted by design of experiments, using a simplex centroid design.
26886338	6	6	theme	factor	801:806	arg1	space					808:812	the factor space	797:812	the factor space where the dressing of optimized quantitative composition was chosen	797:880	The superimposition of the contour plots, calculated by the best fit model for each response variable, permitted to individuate a region of the factor space where the dressing of optimized quantitative composition was chosen.
26886338	2	7	theme	skin	326:329	arg1	ulcers					331:336	chronic skin ulcers	318:336	chronic skin ulcers	318:336	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	7	8	theme	saline	952:957	arg1	amount					923:928	high amount	918:928	high amount of phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect	918:1061	Such a dressing was able to absorb high amount of phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect.
26886338	7	8	theme	saline	952:957	arg1	saline					952:957	phosphate-buffered saline	933:957	phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect	933:1061	Such a dressing was able to absorb high amount of phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect.
26886338	6	9	theme	space	808:812	arg1	region					787:792	a region	785:792	a region of the factor space where the dressing of optimized quantitative composition was chosen	785:880	The superimposition of the contour plots, calculated by the best fit model for each response variable, permitted to individuate a region of the factor space where the dressing of optimized quantitative composition was chosen.
26886338	6	10	theme	quantitative	846:857	arg1	composition					859:869	optimized quantitative composition	836:869	optimized quantitative composition	836:869	The superimposition of the contour plots, calculated by the best fit model for each response variable, permitted to individuate a region of the factor space where the dressing of optimized quantitative composition was chosen.
26886338	8	11	theme	optimized	1068:1076	arg1	formulation					1078:1088	The optimized formulation	1064:1088	The optimized formulation	1064:1088	The optimized formulation was characterized by optimal mechanical properties and by cell proliferation and antioxidant activity on human fibroblast cell line.
26886338	2	12	theme	molecular	267:275	arg1	weight					277:282	high molecular weight	262:282	high molecular weight	262:282	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	2	12	theme	molecular	267:275	arg1	glutamate					251:259	chitosan glutamate	242:259	chitosan glutamate (high molecular weight)	242:283	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	0	13	theme	Skin	100:103	arg1	Ulcers					105:110	Chronic Skin Ulcers	92:110	Chronic Skin Ulcers	92:110	Sponge-Like Dressings Based on the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers.
26886338	5	14	theme	dressings	608:616	arg1	variables					646:654	response variables	637:654	response variables	637:654	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	14	theme	dressings	608:616	arg1	propensity					553:562	hydration propensity	543:562	hydration propensity	543:562	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	14	theme	dressings	608:616	arg1	viscoelasticity					578:592	viscoelasticity	578:592	viscoelasticity	578:592	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	14	theme	dressings	608:616	arg1	resistance					531:540	Mechanical resistance	520:540	Mechanical resistance	520:540	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	14	theme	dressings	608:616	arg1	viscous					565:571	viscous	565:571	viscous	565:571	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	2	15	theme	high	262:265	arg1	weight					277:282	high molecular weight	262:282	high molecular weight	262:282	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	2	15	theme	high	262:265	arg1	glutamate					251:259	chitosan glutamate	242:259	chitosan glutamate (high molecular weight)	242:283	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	0	16	theme	Chronic	92:98	arg1	Ulcers					105:110	Chronic Skin Ulcers	92:110	Chronic Skin Ulcers	92:110	Sponge-Like Dressings Based on the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers.
26886338	4	17	theme	experiments	474:484	arg1	design					464:469	design	464:469	design of experiments	464:484	Dressing development was assisted by design of experiments, using a simplex centroid design.
26886338	7	18	theme	high	918:921	arg1	amount					923:928	high amount	918:928	high amount of phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect	918:1061	Such a dressing was able to absorb high amount of phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect.
26886338	7	18	theme	high	918:921	arg1	saline					952:957	phosphate-buffered saline	933:957	phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect	933:1061	Such a dressing was able to absorb high amount of phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect.
26886338	8	19	theme	human	1195:1199	arg1	line					1217:1220	human fibroblast cell line	1195:1220	human fibroblast cell line	1195:1220	The optimized formulation was characterized by optimal mechanical properties and by cell proliferation and antioxidant activity on human fibroblast cell line.
26886338	5	20	dep	resistance	531:540	arg1	properties					594:603	properties	594:603	properties	594:603	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	8	21	theme	fibroblast	1201:1210	arg1	line					1217:1220	human fibroblast cell line	1195:1220	human fibroblast cell line	1195:1220	The optimized formulation was characterized by optimal mechanical properties and by cell proliferation and antioxidant activity on human fibroblast cell line.
26886338	0	22	theme	Sponge-Like	0:10	arg1	Dressings					12:20	Sponge-Like Dressings	0:20	Sponge-Like Dressings	0:20	Sponge-Like Dressings Based on the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers.
26886338	5	23	theme	response	637:644	arg1	viscous					565:571	viscous	565:571	viscous	565:571	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	23	theme	response	637:644	arg1	viscoelasticity					578:592	viscoelasticity	578:592	viscoelasticity	578:592	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	23	theme	response	637:644	arg1	resistance					531:540	Mechanical resistance	520:540	Mechanical resistance	520:540	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	23	theme	response	637:644	arg1	variables					646:654	response variables	637:654	response variables	637:654	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	23	theme	response	637:644	arg1	propensity					553:562	hydration propensity	543:562	hydration propensity	543:562	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	6	24	theme	plots	692:696	arg1	superimposition					661:675	The superimposition	657:675	The superimposition	657:675	The superimposition of the contour plots, calculated by the best fit model for each response variable, permitted to individuate a region of the factor space where the dressing of optimized quantitative composition was chosen.
26886338	3	25	theme	cryoprotectant	405:418	arg1	agent					420:424	cryoprotectant agent	405:424	cryoprotectant agent	405:424	Dressings were prepared by freeze-drying and glycine was added as cryoprotectant agent.
26886338	3	25	theme	cryoprotectant	405:418	arg1	glycine					384:390	glycine	384:390	glycine	384:390	Dressings were prepared by freeze-drying and glycine was added as cryoprotectant agent.
26886338	7	26	theme	phosphate-buffered	933:950	arg1	saline					952:957	phosphate-buffered saline	933:957	phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect	933:1061	Such a dressing was able to absorb high amount of phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect.
26886338	6	27	theme	contour	684:690	arg1	plots					692:696	the contour plots	680:696	the contour plots	680:696	The superimposition of the contour plots, calculated by the best fit model for each response variable, permitted to individuate a region of the factor space where the dressing of optimized quantitative composition was chosen.
26886338	8	28	theme	cell	1212:1215	arg1	line					1217:1220	human fibroblast cell line	1195:1220	human fibroblast cell line	1195:1220	The optimized formulation was characterized by optimal mechanical properties and by cell proliferation and antioxidant activity on human fibroblast cell line.
26886338	0	29	theme	Ulcers	105:110	arg1	Treatment					79:87	the Treatment	75:87	the Treatment of Chronic Skin Ulcers	75:110	Sponge-Like Dressings Based on the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers.
26886338	6	30	theme	composition	859:869	arg1	dressing					824:831	the dressing	820:831	the dressing of optimized quantitative composition	820:869	The superimposition of the contour plots, calculated by the best fit model for each response variable, permitted to individuate a region of the factor space where the dressing of optimized quantitative composition was chosen.
26886338	7	31	theme	protective	1045:1054	arg1	effect					1056:1061	a protective effect	1043:1061	a protective effect	1043:1061	Such a dressing was able to absorb high amount of phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect.
26886338	4	32	theme	Dressing	427:434	arg1	development					436:446	Dressing development	427:446	Dressing development	427:446	Dressing development was assisted by design of experiments, using a simplex centroid design.
26886338	2	33	theme	dressings	223:231	arg1	development					196:206	the development	192:206	the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers	192:336	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	2	33	theme	dressings	223:231	arg1	aim					164:166	The aim	160:166	The aim of the present work	160:186	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	6	34	theme	optimized	836:844	arg1	composition					859:869	optimized quantitative composition	836:869	optimized quantitative composition	836:869	The superimposition of the contour plots, calculated by the best fit model for each response variable, permitted to individuate a region of the factor space where the dressing of optimized quantitative composition was chosen.
26886338	2	35	theme	sponge-like	211:221	arg1	dressings					223:231	sponge-like dressings	211:231	sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers	211:336	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	0	36	theme	Chitosan	50:57	arg1	Association					35:45	the Association	31:45	the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers	31:110	Sponge-Like Dressings Based on the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers.
26886338	4	37	theme	centroid	503:510	arg1	design					512:517	a simplex centroid design	493:517	a simplex centroid design	493:517	Dressing development was assisted by design of experiments, using a simplex centroid design.
26886338	8	38	theme	optimal	1111:1117	arg1	properties					1130:1139	optimal mechanical properties	1111:1139	optimal mechanical properties	1111:1139	The optimized formulation was characterized by optimal mechanical properties and by cell proliferation and antioxidant activity on human fibroblast cell line.
26886338	7	39	theme	rheological	990:1000	arg1	properties					1002:1011	rheological properties	990:1011	rheological properties enabling both a lubricant and a protective effect	990:1061	Such a dressing was able to absorb high amount of phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect.
26886338	2	40	theme	chronic	318:324	arg1	ulcers					331:336	chronic skin ulcers	318:336	chronic skin ulcers	318:336	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	5	41	theme	hydration	543:551	arg1	viscous					565:571	viscous	565:571	viscous	565:571	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	41	theme	hydration	543:551	arg1	viscoelasticity					578:592	viscoelasticity	578:592	viscoelasticity	578:592	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	41	theme	hydration	543:551	arg1	resistance					531:540	Mechanical resistance	520:540	Mechanical resistance	520:540	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	41	theme	hydration	543:551	arg1	variables					646:654	response variables	637:654	response variables	637:654	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	5	41	theme	hydration	543:551	arg1	propensity					553:562	hydration propensity	543:562	hydration propensity	543:562	Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables.
26886338	8	42	theme	antioxidant	1171:1181	arg1	activity					1183:1190	antioxidant activity	1171:1190	antioxidant activity	1171:1190	The optimized formulation was characterized by optimal mechanical properties and by cell proliferation and antioxidant activity on human fibroblast cell line.
26886338	8	43	theme	mechanical	1119:1128	arg1	properties					1130:1139	optimal mechanical properties	1111:1139	optimal mechanical properties	1111:1139	The optimized formulation was characterized by optimal mechanical properties and by cell proliferation and antioxidant activity on human fibroblast cell line.
26886338	2	44	theme	work	183:186	arg1	development					196:206	the development	192:206	the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers	192:336	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	2	44	theme	work	183:186	arg1	aim					164:166	The aim	160:166	The aim of the present work	160:186	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	2	45	theme	chitosan	242:249	arg1	weight					277:282	high molecular weight	262:282	high molecular weight	262:282	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	2	45	theme	chitosan	242:249	arg1	glutamate					251:259	chitosan glutamate	242:259	chitosan glutamate (high molecular weight)	242:283	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	0	46	theme	Sericin	63:69	arg1	Association					35:45	the Association	31:45	the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers	31:110	Sponge-Like Dressings Based on the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers.
26886338	2	47	theme	present	175:181	arg1	work					183:186	the present work	171:186	the present work	171:186	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	2	48	theme	ulcers	331:336	arg1	treatment					305:313	the treatment	301:313	the treatment of chronic skin ulcers	301:336	The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers.
26886338	6	49	theme	fit	722:724	arg1	model					726:730	the best fit model	713:730	the best fit model for each response variable	713:757	The superimposition of the contour plots, calculated by the best fit model for each response variable, permitted to individuate a region of the factor space where the dressing of optimized quantitative composition was chosen.
26886338	1	50	theme	Experiments-Assisted	126:145	arg1	Development					147:157	Experiments-Assisted Development	126:157	Experiments-Assisted Development	126:157	I. Design of Experiments-Assisted Development.
24526327	6	0	theme	auxin	889:893	arg1	accumulation					895:906	auxin accumulation	889:906	auxin accumulation	889:906	Expression of PIN1 in IPC was always preceded by auxin accumulation.
24526327	11	1	theme	successive	1598:1607	arg1	events					1618:1623	successive cellular events	1598:1623	successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis	1598:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	8	2	theme	middle	1152:1157	arg1	region					1159:1164	the middle region	1148:1164	the middle region of the interfascicular area, located between neighboring vascular bundles	1148:1238	These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles.
24526327	4	3	theme	accumulation	574:585	arg1	appearance					554:563	appearance	554:563	follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions	545:694	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	10	4	theme	walls	1368:1372	arg1	composition					1349:1359	the chemical composition	1336:1359	the chemical composition of IPC walls	1336:1372	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
24526327	1	5	theme	mechanism	176:184	arg1	knowledge					159:167	our knowledge	155:167	our knowledge of the mechanism of secondary growth	155:204	Development of cambium and its activity is important for our knowledge of the mechanism of secondary growth.
24526327	8	6	theme	interfascicular	1173:1187	arg1	area					1189:1192	the interfascicular area	1169:1192	the interfascicular area	1169:1192	These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles.
24526327	4	7	theme	basal	649:653	arg1	membrane					662:669	the basal plasma membrane	645:669	the basal plasma membrane	645:669	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	1	8	theme	secondary	189:197	arg1	growth					199:204	secondary growth	189:204	secondary growth	189:204	Development of cambium and its activity is important for our knowledge of the mechanism of secondary growth.
24526327	4	9	theme	polar	610:614	arg1	accumulation					574:585	auxin accumulation	568:585	auxin accumulation	568:585	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	4	9	theme	polar	610:614	arg1	localization					629:640	polar PIN1 protein localization	610:640	polar PIN1 protein localization	610:640	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	4	10	theme	protein	621:627	arg1	accumulation					574:585	auxin accumulation	568:585	auxin accumulation	568:585	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	4	10	theme	protein	621:627	arg1	localization					629:640	polar PIN1 protein localization	610:640	polar PIN1 protein localization	610:640	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	7	11	theme	periclinal	988:997	arg1	division					999:1006	their periclinal division	982:1006	their periclinal division	982:1006	Basal localization of PIN1 was already established in the cells prior to their periclinal division.
24526327	8	12	theme	vascular	1223:1230	arg1	bundles					1232:1238	neighboring vascular bundles	1211:1238	neighboring vascular bundles	1211:1238	These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles.
24526327	1	13	theme	activity	129:136	arg1	Development					98:108	Development	98:108	Development of cambium and its activity	98:136	Development of cambium and its activity is important for our knowledge of the mechanism of secondary growth.
24526327	9	14	theme	which	1266:1270	arg1	closure					1280:1286	the closure	1276:1286	the closure of the cambial ring within the stem	1276:1322	The final consequence of which was the closure of the cambial ring within the stem.
24526327	9	14	theme	which	1266:1270	arg1	consequence					1251:1261	The final consequence	1241:1261	The final consequence of which	1241:1270	The final consequence of which was the closure of the cambial ring within the stem.
24526327	2	15	theme	model	246:250	arg1	plant					252:256	a good model plant	239:256	a good model plant for such a kind of study	239:281	Arabidopsis thaliana emerges as a good model plant for such a kind of study.
24526327	10	16	from	rich	1465:1468	arg1	HRGPs					1489:1493	HRGPs	1489:1493	HRGPs	1489:1493	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
24526327	10	16	from	rich	1465:1468	arg1	hydroxyproline					1473:1486	hydroxyproline	1473:1486	hydroxyproline (HRGPs)	1473:1494	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
24526327	4	17	theme	periclinal	675:684	arg1	divisions					686:694	periclinal divisions	675:694	periclinal divisions	675:694	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	0	18	theme	Arabidopsis	85:95	arg1	stems					76:80	inflorescence stems	62:80	inflorescence stems of Arabidopsis	62:95	Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis.
24526327	10	19	from	hydroxyproline	1473:1486	arg1	rich					1465:1468	rich	1465:1468	rich	1465:1468	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
24526327	10	20	theme	pectic	1417:1422	arg1	epitopes					1424:1431	pectic epitopes	1417:1431	pectic epitopes	1417:1431	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
24526327	9	21	theme	cambial	1295:1301	arg1	ring					1303:1306	the cambial ring	1291:1306	the cambial ring within the stem	1291:1322	The final consequence of which was the closure of the cambial ring within the stem.
24526327	8	22	theme	cellular	1015:1022	arg1	events					1024:1029	These cellular events	1009:1029	These cellular events	1009:1029	These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles.
24526327	7	23	theme	PIN1	931:934	arg1	localization					915:926	Basal localization	909:926	Basal localization of PIN1	909:934	Basal localization of PIN1 was already established in the cells prior to their periclinal division.
24526327	4	24	from	appearance	554:563	arg1	membrane					662:669	the basal plasma membrane	645:669	the basal plasma membrane	645:669	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	4	24	from	appearance	554:563	arg1	divisions					686:694	periclinal divisions	675:694	periclinal divisions	675:694	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	3	25	theme	thaliana	401:408	arg1	stems					389:393	inflorescence stems	375:393	inflorescence stems of A. thaliana	375:408	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	0	26	theme	Cellular	0:7	arg1	events					9:14	Cellular events	0:14	Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis.	0:96	Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis.
24526327	8	27	theme	parenchyma	1048:1057	arg1	cells					1059:1063	parenchyma cells	1048:1063	parenchyma cells adjacent to the vascular bundles	1048:1096	These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles.
24526327	10	28	from	Changes	1325:1331	arg1	composition					1349:1359	the chemical composition	1336:1359	the chemical composition of IPC walls	1336:1372	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
24526327	11	29	from	regions	1648:1654	arg1	terms					1589:1593	terms	1589:1593	terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis	1589:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	8	30	theme	adjacent	1065:1072	arg1	cells					1059:1063	parenchyma cells	1048:1063	parenchyma cells adjacent to the vascular bundles	1048:1096	These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles.
24526327	6	31	theme	PIN1	854:857	arg1	Expression					840:849	Expression	840:849	Expression of PIN1 in IPC	840:864	Expression of PIN1 in IPC was always preceded by auxin accumulation.
24526327	10	32	theme	extensins	1455:1463	arg1	changes					1406:1412	changes	1406:1412	changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs)	1406:1494	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
24526327	11	33	theme	Arabidopsis	1682:1692	arg1	stems					1673:1677	inflorescence stems	1659:1677	inflorescence stems of Arabidopsis	1659:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	5	34	theme	cambium	769:775	arg1	cells					788:792	cambium parenchyma cells	769:792	cambium parenchyma cells	769:792	Distribution of auxin was observed to be higher in differentiating into cambium parenchyma cells compared to cells within the pith and cortex.
24526327	0	35	theme	cambium	39:45	arg1	ontogenesis					47:57	interfascicular cambium ontogenesis	23:57	interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis	23:95	Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis.
24526327	3	36	theme	inflorescence	375:387	arg1	stems					389:393	inflorescence stems	375:393	inflorescence stems of A. thaliana	375:408	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	11	37	theme	stems	1673:1677	arg1	regions					1648:1654	the interfascicular regions	1628:1654	the interfascicular regions of inflorescence stems of Arabidopsis	1628:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	3	38	from	development	426:436	arg1	cells 					512:517	differentiated interfascicular parenchyma cells 	470:517	differentiated interfascicular parenchyma cells (IPC)	470:522	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	3	38	from	development	426:436	arg1	IPC					519:521	IPC	519:521	IPC	519:521	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	3	39	theme	differentiated	470:483	arg1	cells 					512:517	differentiated interfascicular parenchyma cells 	470:517	differentiated interfascicular parenchyma cells (IPC)	470:522	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	3	39	theme	differentiated	470:483	arg1	IPC					519:521	IPC	519:521	IPC	519:521	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	11	40	from	events	1618:1623	arg1	regions					1648:1654	the interfascicular regions	1628:1654	the interfascicular regions of inflorescence stems of Arabidopsis	1628:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	3	41	theme	cambium	457:463	arg1	development					426:436	the development	422:436	the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC)	422:522	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	0	42	theme	inflorescence	62:74	arg1	stems					76:80	inflorescence stems	62:80	inflorescence stems of Arabidopsis	62:95	Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis.
24526327	4	43	theme	gene	593:596	arg1	accumulation					574:585	auxin accumulation	568:585	auxin accumulation	568:585	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	4	43	theme	gene	593:596	arg1	expression					598:607	PIN1 gene expression	588:607	PIN1 gene expression	588:607	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	3	44	theme	parenchyma	501:510	arg1	cells 					512:517	differentiated interfascicular parenchyma cells 	470:517	differentiated interfascicular parenchyma cells (IPC)	470:522	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	3	44	theme	parenchyma	501:510	arg1	IPC					519:521	IPC	519:521	IPC	519:521	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	11	45	theme	cellular	1609:1616	arg1	events					1618:1623	successive cellular events	1598:1623	successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis	1598:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	3	46	from	cells 	512:517	arg1	development					426:436	the development	422:436	the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC)	422:522	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	3	46	from	cells 	512:517	arg1	cambium					457:463	interfascicular cambium	441:463	interfascicular cambium from differentiated interfascicular parenchyma cells (IPC)	441:522	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	2	47	theme	study	277:281	arg1	kind					269:272	such a kind	262:272	such a kind of study	262:281	Arabidopsis thaliana emerges as a good model plant for such a kind of study.
24526327	11	48	theme	cambium	1566:1572	arg1	ontogenesis					1574:1584	interfascicular cambium ontogenesis	1550:1584	interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis	1550:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	4	49	theme	auxin	568:572	arg1	accumulation					574:585	auxin accumulation	568:585	auxin accumulation	568:585	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	4	49	theme	auxin	568:572	arg1	localization					629:640	polar PIN1 protein localization	610:640	polar PIN1 protein localization	610:640	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	4	49	theme	auxin	568:572	arg1	expression					598:607	PIN1 gene expression	588:607	PIN1 gene expression	588:607	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	10	50	theme	IPC	1364:1366	arg1	walls					1368:1372	IPC walls	1364:1372	IPC walls	1364:1372	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
24526327	11	51	from	ontogenesis	1574:1584	arg1	terms					1589:1593	terms	1589:1593	terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis	1589:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	6	52	from	Expression	840:849	arg1	IPC					862:864	IPC	862:864	IPC	862:864	Expression of PIN1 in IPC was always preceded by auxin accumulation.
24526327	11	53	theme	interfascicular	1632:1646	arg1	regions					1648:1654	the interfascicular regions	1628:1654	the interfascicular regions of inflorescence stems of Arabidopsis	1628:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	11	54	from	terms	1589:1593	arg1	regions					1648:1654	the interfascicular regions	1628:1654	the interfascicular regions of inflorescence stems of Arabidopsis	1628:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	1	55	theme	growth	199:204	arg1	mechanism					176:184	the mechanism	172:184	the mechanism of secondary growth	172:204	Development of cambium and its activity is important for our knowledge of the mechanism of secondary growth.
24526327	8	56	theme	area	1189:1192	arg1	region					1159:1164	the middle region	1148:1164	the middle region of the interfascicular area, located between neighboring vascular bundles	1148:1238	These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles.
24526327	2	57	theme	good	241:244	arg1	plant					252:256	a good model plant	239:256	a good model plant for such a kind of study	239:281	Arabidopsis thaliana emerges as a good model plant for such a kind of study.
24526327	8	58	theme	located	1195:1201	arg1	area					1189:1192	the interfascicular area	1169:1192	the interfascicular area	1169:1192	These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles.
24526327	4	59	theme	PIN1	616:619	arg1	accumulation					574:585	auxin accumulation	568:585	auxin accumulation	568:585	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	4	59	theme	PIN1	616:619	arg1	localization					629:640	polar PIN1 protein localization	610:640	polar PIN1 protein localization	610:640	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	9	60	theme	final	1245:1249	arg1	closure					1280:1286	the closure	1276:1286	the closure of the cambial ring within the stem	1276:1322	The final consequence of which was the closure of the cambial ring within the stem.
24526327	9	60	theme	final	1245:1249	arg1	consequence					1251:1261	The final consequence	1241:1261	The final consequence of which	1241:1270	The final consequence of which was the closure of the cambial ring within the stem.
24526327	8	61	theme	neighboring	1211:1221	arg1	bundles					1232:1238	neighboring vascular bundles	1211:1238	neighboring vascular bundles	1211:1238	These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles.
24526327	7	62	theme	prior	973:977	arg1	cells					967:971	the cells	963:971	the cells prior to their periclinal division	963:1006	Basal localization of PIN1 was already established in the cells prior to their periclinal division.
24526327	3	63	theme	cellular	312:319	arg1	events					321:326	cellular events	312:326	cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana	312:408	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	4	64	theme	plasma	655:660	arg1	membrane					662:669	the basal plasma membrane	645:669	the basal plasma membrane	645:669	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	10	65	theme	epitopes	1424:1431	arg1	changes					1406:1412	changes	1406:1412	changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs)	1406:1494	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
24526327	5	66	theme	auxin	713:717	arg1	Distribution					697:708	Distribution	697:708	Distribution of auxin	697:717	Distribution of auxin was observed to be higher in differentiating into cambium parenchyma cells compared to cells within the pith and cortex.
24526327	4	67	dep	follows	545:551	arg1	appearance					554:563	appearance	554:563	follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions	545:694	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	0	68	from	ontogenesis	47:57	arg1	stems					76:80	inflorescence stems	62:80	inflorescence stems of Arabidopsis	62:95	Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis.
24526327	9	69	theme	ring	1303:1306	arg1	closure					1280:1286	the closure	1276:1286	the closure of the cambial ring within the stem	1276:1322	The final consequence of which was the closure of the cambial ring within the stem.
24526327	9	69	theme	ring	1303:1306	arg1	consequence					1251:1261	The final consequence	1241:1261	The final consequence of which	1241:1270	The final consequence of which was the closure of the cambial ring within the stem.
24526327	10	70	theme	xyloglucans	1434:1444	arg1	changes					1406:1412	changes	1406:1412	changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs)	1406:1494	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
24526327	0	71	theme	interfascicular	23:37	arg1	ontogenesis					47:57	interfascicular cambium ontogenesis	23:57	interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis	23:95	Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis.
24526327	7	72	theme	Basal	909:913	arg1	localization					915:926	Basal localization	909:926	Basal localization of PIN1	909:934	Basal localization of PIN1 was already established in the cells prior to their periclinal division.
24526327	1	73	theme	cambium	113:119	arg1	Development					98:108	Development	98:108	Development of cambium and its activity	98:136	Development of cambium and its activity is important for our knowledge of the mechanism of secondary growth.
24526327	3	74	theme	interfascicular	348:362	arg1	regions					364:370	the interfascicular regions	344:370	the interfascicular regions of inflorescence stems of A. thaliana	344:408	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	10	75	theme	rich	1465:1468	arg1	extensins					1455:1463	extensins	1455:1463	extensins rich in hydroxyproline (HRGPs)	1455:1494	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
24526327	3	76	theme	interfascicular	441:455	arg1	cambium					457:463	interfascicular cambium	441:463	interfascicular cambium from differentiated interfascicular parenchyma cells (IPC)	441:522	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	8	77	theme	vascular	1081:1088	arg1	bundles					1090:1096	the vascular bundles	1077:1096	the vascular bundles	1077:1096	These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles.
24526327	3	78	theme	A.	398:399	arg1	thaliana					401:408	A. thaliana	398:408	A. thaliana	398:408	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	11	79	theme	inflorescence	1659:1671	arg1	stems					1673:1677	inflorescence stems	1659:1677	inflorescence stems of Arabidopsis	1659:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	11	80	theme	interfascicular	1550:1564	arg1	ontogenesis					1574:1584	interfascicular cambium ontogenesis	1550:1584	interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis	1550:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	5	81	theme	parenchyma	777:786	arg1	cells					788:792	cambium parenchyma cells	769:792	cambium parenchyma cells	769:792	Distribution of auxin was observed to be higher in differentiating into cambium parenchyma cells compared to cells within the pith and cortex.
24526327	3	82	theme	stems	389:393	arg1	regions					364:370	the interfascicular regions	344:370	the interfascicular regions of inflorescence stems of A. thaliana	344:408	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	3	83	theme	interfascicular	485:499	arg1	cells 					512:517	differentiated interfascicular parenchyma cells 	470:517	differentiated interfascicular parenchyma cells (IPC)	470:522	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	3	83	theme	interfascicular	485:499	arg1	IPC					519:521	IPC	519:521	IPC	519:521	Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC).
24526327	4	84	theme	PIN1	588:591	arg1	accumulation					574:585	auxin accumulation	568:585	auxin accumulation	568:585	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	4	84	theme	PIN1	588:591	arg1	expression					598:607	PIN1 gene expression	588:607	PIN1 gene expression	588:607	These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions.
24526327	11	85	theme	events	1618:1623	arg1	terms					1589:1593	terms	1589:1593	terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis	1589:1692	In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
24526327	5	86	dep	pith	823:826	arg1	the					819:821	the	819:821	the	819:821	Distribution of auxin was observed to be higher in differentiating into cambium parenchyma cells compared to cells within the pith and cortex.
24526327	10	87	theme	chemical	1340:1347	arg1	composition					1349:1359	the chemical composition	1336:1359	the chemical composition of IPC walls	1336:1372	Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
26987354	0	0	theme	rats	78:81	arg1	metabolism					64:73	metabolism	64:73	metabolism of rats receiving high-fat diet	64:105	Effects of beta-glucans ingestion (Saccharomyces cerevisiae) on metabolism of rats receiving high-fat diet.
26987354	9	1	theme	variance	1072:1079	arg1	analysis					1060:1067	analysis	1060:1067	analysis of variance (anova)	1060:1087	Data were submitted to analysis of variance (anova).
26987354	12	2	theme	high-fat	1599:1606	arg1	diet					1608:1611	high-fat diet	1599:1611	high-fat diet	1599:1611	BG from S. cerevisiae promoted beneficial metabolic effects in rats receiving high-fat diet.
26987354	4	3	theme	period	534:539	arg1	end					522:524	the end	518:524	the end of this period	518:539	At the end of this period, body weight, blood glucose and Lee index were assessed.
26987354	6	4	theme	BG	857:858	arg1	30 mg/kg					845:852	30 mg/kg	845:852	30 mg/kg of BG dissolved in saline solution by gavage	845:897	Animals from groups CB and OB received 30 mg/kg of BG dissolved in saline solution by gavage.
26987354	6	5	from	groups	819:824	arg1	Animals					806:812	Animals	806:812	Animals from groups CB and OB	806:834	Animals from groups CB and OB received 30 mg/kg of BG dissolved in saline solution by gavage.
26987354	8	6	theme	factorial	1019:1027	arg1	scheme					1029:1034	2 × 2 factorial scheme	1013:1034	2 × 2 factorial scheme	1013:1034	The design used was totally randomized in 2 × 2 factorial scheme.
26987354	0	7	theme	high-fat	93:100	arg1	diet					102:105	high-fat diet	93:105	high-fat diet	93:105	Effects of beta-glucans ingestion (Saccharomyces cerevisiae) on metabolism of rats receiving high-fat diet.
26987354	0	8	from	Effects	0:6	arg1	metabolism					64:73	metabolism	64:73	metabolism of rats receiving high-fat diet	64:105	Effects of beta-glucans ingestion (Saccharomyces cerevisiae) on metabolism of rats receiving high-fat diet.
26987354	10	9	theme	cholesterol	1155:1165	arg1	p < 0.05					1136:1143	p < 0.05	1136:1143	p < 0.05	1136:1143	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	10	9	theme	cholesterol	1155:1165	arg1	levels					1128:1133	inferior levels	1119:1133	inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%)	1119:1228	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	5	10	theme	obese	775:779	arg1	animals					781:787	(OB) - obese animals	768:787	(OB) - obese animals	768:787	In the second stage, those 24 animals were redivided into four groups: (C) - control diet; (CB) - control diet and treated with Beta-glucan (BG); (O) - obese animals and (OB) - obese animals treated with BG.
26987354	4	11	theme	body	542:545	arg1	weight					547:552	body weight	542:552	body weight	542:552	At the end of this period, body weight, blood glucose and Lee index were assessed.
26987354	10	12	theme	triacylglycerols	1177:1192	arg1	p < 0.05					1136:1143	p < 0.05	1136:1143	p < 0.05	1136:1143	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	10	12	theme	triacylglycerols	1177:1192	arg1	levels					1128:1133	inferior levels	1119:1133	inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%)	1119:1228	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	10	13	theme	total	1149:1153	arg1	cholesterol					1155:1165	total cholesterol	1149:1165	total cholesterol (13.33%)	1149:1174	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	10	13	theme	total	1149:1153	arg1	%					1173:1173	13.33%	1168:1173	13.33%	1168:1173	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	2	14	theme	first	313:317	arg1	one					319:321	the first one	309:321	the first one	309:321	The experimental period was divided into two stages: in the first one, the animals were divided into two groups containing 12 animals each.
26987354	10	15	from	O	1270:1270	arg1	animals					1251:1257	the animals	1247:1257	the animals from group O	1247:1270	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	6	16	dep	groups	819:824	arg1	CB					826:827	CB	826:827	CB	826:827	Animals from groups CB and OB received 30 mg/kg of BG dissolved in saline solution by gavage.
26987354	6	16	dep	groups	819:824	arg1	groups					819:824	groups CB and OB	819:834	groups CB and OB	819:834	Animals from groups CB and OB received 30 mg/kg of BG dissolved in saline solution by gavage.
26987354	6	16	dep	groups	819:824	arg1	OB					833:834	OB	833:834	OB	833:834	Animals from groups CB and OB received 30 mg/kg of BG dissolved in saline solution by gavage.
26987354	10	17	theme	glucose	1213:1219	arg1	p < 0.05					1136:1143	p < 0.05	1136:1143	p < 0.05	1136:1143	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	10	17	theme	glucose	1213:1219	arg1	levels					1128:1133	inferior levels	1119:1133	inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%)	1119:1228	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	3	18	theme	fat	495:497	arg1	%					485:485	20%	483:485	20% of pork fat	483:497	The first group received commercial feed and the second received high-fat diet containing 20% of pork fat during 60 days.
26987354	3	18	theme	fat	495:497	arg1	fat					495:497	pork fat	490:497	pork fat	490:497	The first group received commercial feed and the second received high-fat diet containing 20% of pork fat during 60 days.
26987354	3	19	theme	first	397:401	arg1	group					403:407	The first group	393:407	The first group	393:407	The first group received commercial feed and the second received high-fat diet containing 20% of pork fat during 60 days.
26987354	1	20	theme	cerevisiae	167:176	arg1	ingestion					179:187	beta-glucans (Saccharomyces cerevisiae) ingestion	139:187	beta-glucans (Saccharomyces cerevisiae) ingestion	139:187	We investigated the effects of beta-glucans (Saccharomyces cerevisiae) ingestion on metabolic parameters of Wistar rats receiving high-fat diet.
26987354	3	21	contain	containing	472:481	arg1	diet					467:470	high-fat diet	458:470	high-fat diet containing 20% of pork fat during 60 days	458:512	The first group received commercial feed and the second received high-fat diet containing 20% of pork fat during 60 days.
26987354	3	21	contain	containing	472:481	arg2	fat					495:497	pork fat	490:497	pork fat	490:497	The first group received commercial feed and the second received high-fat diet containing 20% of pork fat during 60 days.
26987354	3	21	contain	containing	472:481	arg2	%					485:485	20%	483:485	20% of pork fat	483:497	The first group received commercial feed and the second received high-fat diet containing 20% of pork fat during 60 days.
26987354	10	22	theme	blood	1207:1211	arg1	%					1227:1227	23.97%	1222:1227	23.97%	1222:1227	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	10	22	theme	blood	1207:1211	arg1	glucose					1213:1219	blood glucose	1207:1219	blood glucose (23.97%)	1207:1228	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	3	23	theme	commercial	418:427	arg1	feed					429:432	commercial feed	418:432	commercial feed	418:432	The first group received commercial feed and the second received high-fat diet containing 20% of pork fat during 60 days.
26987354	1	24	from	effects	128:134	arg1	parameters					202:211	metabolic parameters	192:211	metabolic parameters of Wistar rats receiving high-fat diet	192:250	We investigated the effects of beta-glucans (Saccharomyces cerevisiae) ingestion on metabolic parameters of Wistar rats receiving high-fat diet.
26987354	11	25	from	increase	1308:1315	arg1	p < 0.05					1331:1338	p < 0.05	1331:1338	p < 0.05	1331:1338	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	25	from	increase	1308:1315	arg1	index					1324:1328	Lee index	1320:1328	Lee index (p < 0.05)	1320:1339	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	10	26	from	group	1106:1110	arg1	Animals					1090:1096	Animals	1090:1096	Animals from OB group	1090:1110	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	1	27	theme	ingestion	179:187	arg1	effects					128:134	the effects	124:134	the effects of beta-glucans (Saccharomyces cerevisiae) ingestion on metabolic parameters of Wistar rats receiving high-fat diet	124:250	We investigated the effects of beta-glucans (Saccharomyces cerevisiae) ingestion on metabolic parameters of Wistar rats receiving high-fat diet.
26987354	7	28	dep	groups	913:918	arg1	groups					913:918	groups C and O	913:926	groups C and O	913:926	Animals from groups C and O received only saline solution for 28 days.
26987354	7	28	dep	groups	913:918	arg1	O					926:926	O	926:926	O	926:926	Animals from groups C and O received only saline solution for 28 days.
26987354	7	28	dep	groups	913:918	arg1	C					920:920	C	920:920	C	920:920	Animals from groups C and O received only saline solution for 28 days.
26987354	12	29	theme	metabolic	1563:1571	arg1	effects					1573:1579	beneficial metabolic effects	1552:1579	beneficial metabolic effects	1552:1579	BG from S. cerevisiae promoted beneficial metabolic effects in rats receiving high-fat diet.
26987354	5	30	theme	OB	769:770	arg1	animals					781:787	(OB) - obese animals	768:787	(OB) - obese animals	768:787	In the second stage, those 24 animals were redivided into four groups: (C) - control diet; (CB) - control diet and treated with Beta-glucan (BG); (O) - obese animals and (OB) - obese animals treated with BG.
26987354	0	31	theme	ingestion	24:32	arg1	Effects					0:6	Effects	0:6	Effects of beta-glucans ingestion (Saccharomyces cerevisiae) on metabolism of rats receiving high-fat diet.	0:106	Effects of beta-glucans ingestion (Saccharomyces cerevisiae) on metabolism of rats receiving high-fat diet.
26987354	11	32	theme	stomach	1502:1508	arg1	organs					1402:1407	organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology	1402:1518	organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology	1402:1518	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	32	theme	stomach	1502:1508	arg1	histology					1510:1518	stomach histology	1502:1518	stomach histology	1502:1518	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	1	33	theme	metabolic	192:200	arg1	parameters					202:211	metabolic parameters	192:211	metabolic parameters of Wistar rats receiving high-fat diet	192:250	We investigated the effects of beta-glucans (Saccharomyces cerevisiae) ingestion on metabolic parameters of Wistar rats receiving high-fat diet.
26987354	0	34	theme	beta-glucans	11:22	arg1	ingestion					24:32	beta-glucans ingestion	11:32	beta-glucans ingestion (Saccharomyces cerevisiae)	11:59	Effects of beta-glucans ingestion (Saccharomyces cerevisiae) on metabolism of rats receiving high-fat diet.
26987354	12	35	from	cerevisiae	1532:1541	arg1	BG					1521:1522	BG	1521:1522	BG from S. cerevisiae	1521:1541	BG from S. cerevisiae promoted beneficial metabolic effects in rats receiving high-fat diet.
26987354	11	36	theme	carcass	1436:1442	arg1	composition					1444:1454	carcass composition	1436:1454	carcass composition	1436:1454	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	36	theme	carcass	1436:1442	arg1	organs					1402:1407	organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology	1402:1518	organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology	1402:1518	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	5	37	theme	control	675:681	arg1	diet					683:686	control diet;	675:687	control diet; (CB)	675:692	In the second stage, those 24 animals were redivided into four groups: (C) - control diet; (CB) - control diet and treated with Beta-glucan (BG); (O) - obese animals and (OB) - obese animals treated with BG.
26987354	5	37	theme	control	675:681	arg1	CB					690:691	CB	690:691	CB	690:691	In the second stage, those 24 animals were redivided into four groups: (C) - control diet; (CB) - control diet and treated with Beta-glucan (BG); (O) - obese animals and (OB) - obese animals treated with BG.
26987354	12	38	theme	beneficial	1552:1561	arg1	effects					1573:1579	beneficial metabolic effects	1552:1579	beneficial metabolic effects	1552:1579	BG from S. cerevisiae promoted beneficial metabolic effects in rats receiving high-fat diet.
26987354	11	39	from	alteration	1360:1369	arg1	feed					1374:1377	feed	1374:1377	feed	1374:1377	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	39	from	alteration	1360:1369	arg1	consumption					1389:1399	water consumption	1383:1399	water consumption	1383:1399	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	7	40	theme	saline	942:947	arg1	solution					949:956	saline solution	942:956	saline solution for 28 days	942:968	Animals from groups C and O received only saline solution for 28 days.
26987354	11	41	theme	villus/crypt	1457:1468	arg1	organs					1402:1407	organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology	1402:1518	organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology	1402:1518	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	41	theme	villus/crypt	1457:1468	arg1	ratio					1470:1474	villus/crypt ratio	1457:1474	villus/crypt ratio	1457:1474	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	2	42	contain	containing	365:374	arg1	groups					358:363	two groups	354:363	two groups containing 12 animals each	354:390	The experimental period was divided into two stages: in the first one, the animals were divided into two groups containing 12 animals each.
26987354	2	42	contain	containing	365:374	arg2	animals					379:385	12 animals	376:385	12 animals each	376:390	The experimental period was divided into two stages: in the first one, the animals were divided into two groups containing 12 animals each.
26987354	2	43	dep	animals	379:385	arg1	each					387:390	each	387:390	each	387:390	The experimental period was divided into two stages: in the first one, the animals were divided into two groups containing 12 animals each.
26987354	10	44	theme	inferior	1119:1126	arg1	p < 0.05					1136:1143	p < 0.05	1136:1143	p < 0.05	1136:1143	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	10	44	theme	inferior	1119:1126	arg1	levels					1128:1133	inferior levels	1119:1133	inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%)	1119:1228	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	1	45	theme	Wistar	216:221	arg1	rats					223:226	Wistar rats	216:226	Wistar rats receiving high-fat diet	216:250	We investigated the effects of beta-glucans (Saccharomyces cerevisiae) ingestion on metabolic parameters of Wistar rats receiving high-fat diet.
26987354	8	46	theme	2 × 2	1013:1017	arg1	scheme					1029:1034	2 × 2 factorial scheme	1013:1034	2 × 2 factorial scheme	1013:1034	The design used was totally randomized in 2 × 2 factorial scheme.
26987354	7	47	from	groups	913:918	arg1	Animals					900:906	Animals	900:906	Animals from groups C and O	900:926	Animals from groups C and O received only saline solution for 28 days.
26987354	5	48	theme	second	605:610	arg1	stage					612:616	the second stage	601:616	the second stage	601:616	In the second stage, those 24 animals were redivided into four groups: (C) - control diet; (CB) - control diet and treated with Beta-glucan (BG); (O) - obese animals and (OB) - obese animals treated with BG.
26987354	10	49	theme	group	1264:1268	arg1	O					1270:1270	group O	1264:1270	group O	1264:1270	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	11	50	theme	smaller	1300:1306	arg1	increase					1308:1315	smaller increase	1300:1315	smaller increase in Lee index (p < 0.05)	1300:1339	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	1	51	theme	beta-glucans	139:150	arg1	ingestion					179:187	beta-glucans (Saccharomyces cerevisiae) ingestion	139:187	beta-glucans (Saccharomyces cerevisiae) ingestion	139:187	We investigated the effects of beta-glucans (Saccharomyces cerevisiae) ingestion on metabolic parameters of Wistar rats receiving high-fat diet.
26987354	11	52	theme	BG	1284:1285	arg1	use					1277:1279	The use	1273:1279	The use of BG	1273:1285	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	5	53	theme	obese	750:754	arg1	animals					756:762	obese animals	750:762	obese animals	750:762	In the second stage, those 24 animals were redivided into four groups: (C) - control diet; (CB) - control diet and treated with Beta-glucan (BG); (O) - obese animals and (OB) - obese animals treated with BG.
26987354	0	54	dep	ingestion	24:32	arg1	cerevisiae					49:58	Saccharomyces cerevisiae	35:58	Saccharomyces cerevisiae	35:58	Effects of beta-glucans ingestion (Saccharomyces cerevisiae) on metabolism of rats receiving high-fat diet.
26987354	10	55	theme	OB	1103:1104	arg1	group					1106:1110	OB group	1103:1110	OB group	1103:1110	Animals from OB group showed inferior levels (p < 0.05) of total cholesterol (13.33%), triacylglycerols (16.77%) and blood glucose (23.97%) when compared to the animals from group O.
26987354	1	56	theme	rats	223:226	arg1	parameters					202:211	metabolic parameters	192:211	metabolic parameters of Wistar rats receiving high-fat diet	192:250	We investigated the effects of beta-glucans (Saccharomyces cerevisiae) ingestion on metabolic parameters of Wistar rats receiving high-fat diet.
26987354	11	57	dep	organs	1402:1407	arg1	organs					1402:1407	organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology	1402:1518	organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology	1402:1518	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	57	dep	organs	1402:1407	arg1	pancreas					1481:1488	pancreas	1481:1488	pancreas	1481:1488	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	57	dep	organs	1402:1407	arg1	weight					1409:1414	weight	1409:1414	weight	1409:1414	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	57	dep	organs	1402:1407	arg1	composition					1444:1454	carcass composition	1436:1454	carcass composition	1436:1454	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	57	dep	organs	1402:1407	arg1	HDL-C					1417:1421	HDL-C	1417:1421	HDL-C	1417:1421	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	57	dep	organs	1402:1407	arg1	LDL+VLDL-C					1424:1433	LDL+VLDL-C	1424:1433	LDL+VLDL-C	1424:1433	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	57	dep	organs	1402:1407	arg1	ratio					1470:1474	villus/crypt ratio	1457:1474	villus/crypt ratio	1457:1474	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	2	58	theme	experimental	257:268	arg1	period					270:275	The experimental period	253:275	The experimental period	253:275	The experimental period was divided into two stages: in the first one, the animals were divided into two groups containing 12 animals each.
26987354	11	59	theme	Lee	1320:1322	arg1	p < 0.05					1331:1338	p < 0.05	1331:1338	p < 0.05	1331:1338	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	11	59	theme	Lee	1320:1322	arg1	index					1324:1328	Lee index	1320:1328	Lee index (p < 0.05)	1320:1339	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	3	60	theme	high-fat	458:465	arg1	diet					467:470	high-fat diet	458:470	high-fat diet containing 20% of pork fat during 60 days	458:512	The first group received commercial feed and the second received high-fat diet containing 20% of pork fat during 60 days.
26987354	11	61	theme	water	1383:1387	arg1	consumption					1389:1399	water consumption	1383:1399	water consumption	1383:1399	The use of BG has provided smaller increase in Lee index (p < 0.05), without promoting alteration in feed and water consumption, organs weight, HDL-C, LDL+VLDL-C, carcass composition, villus/crypt ratio, and pancreas, kidney and stomach histology.
26987354	4	62	theme	Lee	573:575	arg1	index					577:581	Lee index	573:581	Lee index	573:581	At the end of this period, body weight, blood glucose and Lee index were assessed.
26987354	6	63	theme	saline	873:878	arg1	solution					880:887	saline solution	873:887	saline solution	873:887	Animals from groups CB and OB received 30 mg/kg of BG dissolved in saline solution by gavage.
26987354	1	64	theme	high-fat	238:245	arg1	diet					247:250	high-fat diet	238:250	high-fat diet	238:250	We investigated the effects of beta-glucans (Saccharomyces cerevisiae) ingestion on metabolic parameters of Wistar rats receiving high-fat diet.
26987354	5	65	dep	redivided	641:649	arg1	C					670:670	C	670:670	C	670:670	In the second stage, those 24 animals were redivided into four groups: (C) - control diet; (CB) - control diet and treated with Beta-glucan (BG); (O) - obese animals and (OB) - obese animals treated with BG.
26987354	5	65	dep	redivided	641:649	arg1	animals					756:762	obese animals	750:762	obese animals	750:762	In the second stage, those 24 animals were redivided into four groups: (C) - control diet; (CB) - control diet and treated with Beta-glucan (BG); (O) - obese animals and (OB) - obese animals treated with BG.
26987354	5	65	dep	redivided	641:649	arg1	animals					781:787	(OB) - obese animals	768:787	(OB) - obese animals	768:787	In the second stage, those 24 animals were redivided into four groups: (C) - control diet; (CB) - control diet and treated with Beta-glucan (BG); (O) - obese animals and (OB) - obese animals treated with BG.
26987354	5	66	theme	control	696:702	arg1	diet					704:707	control diet	696:707	control diet	696:707	In the second stage, those 24 animals were redivided into four groups: (C) - control diet; (CB) - control diet and treated with Beta-glucan (BG); (O) - obese animals and (OB) - obese animals treated with BG.
26987354	3	67	theme	pork	490:493	arg1	fat					495:497	pork fat	490:497	pork fat	490:497	The first group received commercial feed and the second received high-fat diet containing 20% of pork fat during 60 days.
26987354	4	68	theme	blood	555:559	arg1	glucose					561:567	blood glucose	555:567	blood glucose	555:567	At the end of this period, body weight, blood glucose and Lee index were assessed.
24502385	4	0	theme	hydrogenated	838:849	arg1	fat					861:863	hydrogenated vegetable fat	838:863	hydrogenated vegetable fat	838:863	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	3	1	from	presence	510:517	arg1	effect					450:455	the effect	446:455	the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring	446:669	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	13	2	theme	vegetable	1975:1983	arg1	fat					1985:1987	hydrogenated vegetable fat	1962:1987	hydrogenated vegetable fat	1962:1987	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	4	3	dep	groups	738:743	arg1	C					760:760	C	760:760	C	760:760	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	3	dep	groups	738:743	arg1	groups					738:743	four groups	733:743	four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF)	733:970	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	3	dep	groups	738:743	arg1	diet					819:822	diet	819:822	diet enriched with hydrogenated vegetable fat, rich in TFA (T)	819:880	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	3	dep	groups	738:743	arg1	diet					885:888	diet	885:888	diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF)	885:970	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	3	dep	groups	738:743	arg1	diet					754:757	control diet	746:757	control diet (C)	746:761	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	3	dep	groups	738:743	arg1	diet					772:775	control diet	764:775	control diet supplemented with 10% oligofructose (CF)	764:816	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	3	4	from	absence	522:528	arg1	effect					450:455	the effect	446:455	the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring	446:669	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	9	5	theme	statistical	1318:1328	arg1	analysis					1330:1337	the statistical analysis	1314:1337	the statistical analysis	1314:1337	Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures.
24502385	11	6	theme	body	1556:1559	arg1	gain					1568:1571	body weight gain	1556:1571	body weight gain	1556:1571	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	9	7	theme	mean	1305:1308	arg1	error					1292:1296	mean ± standard error	1276:1296	mean ± standard error of the mean	1276:1308	Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures.
24502385	9	7	theme	mean	1305:1308	arg1	Data					1253:1256	Data	1253:1256	Data	1253:1256	Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures.
24502385	11	8	theme	oligofructose	1468:1480	arg1	supplementation					1488:1502	the oligofructose (10%) supplementation	1464:1502	the oligofructose (10%) supplementation during pregnancy and lactation	1464:1533	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	3	9	theme	vegetable	546:554	arg1	fat					556:558	hydrogenated vegetable fat	533:558	hydrogenated vegetable fat	533:558	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	3	10	dep	development	598:608	arg1	the					594:596	the	594:596	the	594:596	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	4	11	from	METHODS	672:678	arg1	day					693:695	the first day	683:695	the first day of pregnancy rats	683:713	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	1	12	theme	trans	198:202	arg1	acids					210:214	trans fatty acids	198:214	trans fatty acids	198:214	BACKGROUND Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring.
24502385	12	13	theme	C	1813:1813	arg1	group					1815:1819	C group	1813:1819	C group	1813:1819	on faeces of the CF group in relation to C group.
24502385	0	14	theme	pups	140:143	arg1	properties					117:126	the intestinal properties	102:126	the intestinal properties of 21-d-old pups	102:143	Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups.
24502385	13	15	theme	independent	1933:1943	arg1	pregnancy					1908:1916	pregnancy	1908:1916	pregnancy	1908:1916	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	2	16	theme	other	307:311	arg1	hand					313:316	the other hand	303:316	the other hand	303:316	On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations.
24502385	9	17	theme	two-way	1361:1367	arg1	ANOVA					1355:1359	ANOVA two-way	1355:1367	ANOVA two-way	1355:1367	Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures.
24502385	13	18	theme	21d-old	2121:2127	arg1	pups					2129:2132	21d-old pups	2121:2132	21d-old pups	2121:2132	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	1	19	from	status	275:280	arg1	offspring					289:297	the offspring	285:297	the offspring	285:297	BACKGROUND Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring.
24502385	13	20	theme	addition	1948:1955	arg1	independent					1933:1943	independent	1933:1943	independent	1933:1943	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	12	21	theme	group	1792:1796	arg1	faeces					1775:1780	faeces	1775:1780	faeces of the CF group in relation to C group	1775:1819	on faeces of the CF group in relation to C group.
24502385	4	22	theme	rich	866:869	arg1	fat					861:863	hydrogenated vegetable fat	838:863	hydrogenated vegetable fat	838:863	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	8	23	theme	Bacterial	1188:1196	arg1	present					1202:1208	Bacterial DNA present	1188:1208	Bacterial DNA present in faeces	1188:1218	Bacterial DNA present in faeces was determined by real-time PCR.
24502385	11	24	theme	21-day-old	1665:1674	arg1	offspring					1676:1684	the 21-day-old offspring	1661:1684	the 21-day-old offspring	1661:1684	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	4	25	from	TFA	874:876	arg1	rich					866:869	rich	866:869	rich	866:869	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	9	26	theme	repeated	1383:1390	arg1	measures					1392:1399	repeated measures	1383:1399	repeated measures	1383:1399	Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures.
24502385	4	27	from	rich	866:869	arg1	T					879:879	T	879:879	T	879:879	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	27	from	rich	866:869	arg1	TFA					874:876	TFA	874:876	TFA (T)	874:880	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	11	28	from	levels	1743:1748	arg1	LPS					1723:1725	serum LPS	1717:1725	serum LPS	1717:1725	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	4	29	theme	pregnancy	700:708	arg1	rats					710:713	pregnancy rats	700:713	pregnancy rats	700:713	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	11	30	theme	TF	1609:1610	arg1	group					1612:1616	the CF and TF group	1598:1616	group	1612:1616	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	7	31	theme	acids	1143:1147	arg1	concentrations					1106:1119	Serum concentrations	1100:1119	Serum concentrations of LPS and free fatty acids (FFA)	1100:1153	Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits.
24502385	3	32	theme	bacterial	627:635	arg1	composition					637:647	bacterial composition	627:647	bacterial composition	627:647	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	11	33	theme	T	1636:1636	arg1	group					1638:1642	T group	1636:1642	T group	1636:1642	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	7	34	theme	free	1132:1135	arg1	FFA					1150:1152	FFA	1150:1152	FFA	1150:1152	Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits.
24502385	7	34	theme	free	1132:1135	arg1	acids					1143:1147	free fatty acids	1132:1147	free fatty acids (FFA)	1132:1153	Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits.
24502385	13	35	theme	oligofructose	1887:1899	arg1	oligofructose					1887:1899	oligofructose	1887:1899	oligofructose	1887:1899	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	13	35	theme	oligofructose	1887:1899	arg1	%					1882:1882	10%	1880:1882	10% of oligofructose	1880:1899	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	8	36	from	present	1202:1208	arg1	faeces					1213:1218	faeces	1213:1218	faeces	1213:1218	Bacterial DNA present in faeces was determined by real-time PCR.
24502385	13	37	theme	lipopolysaccharides	2098:2116	arg1	concentrations					2080:2093	the serum concentrations	2070:2093	the serum concentrations of lipopolysaccharides in 21d-old pups	2070:2132	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	11	38	from	length	1574:1579	arg1	group					1612:1616	the CF and TF group	1598:1616	group	1612:1616	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	11	38	from	length	1574:1579	arg1	group					1638:1642	T group	1636:1642	T group	1636:1642	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	8	39	theme	real-time	1238:1246	arg1	PCR					1248:1250	real-time PCR	1238:1250	real-time PCR	1238:1250	Bacterial DNA present in faeces was determined by real-time PCR.
24502385	0	40	theme	intestinal	106:115	arg1	properties					117:126	the intestinal properties	102:126	the intestinal properties of 21-d-old pups	102:143	Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups.
24502385	13	41	theme	diet	1854:1857	arg1	supplementation					1859:1873	dam's diet supplementation	1848:1873	dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat,	1848:1988	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	4	42	theme	hydrogenated	904:915	arg1	fat					927:929	hydrogenated vegetable fat	904:929	hydrogenated vegetable fat supplemented with 10% oligofructose (TF)	904:970	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	43	theme	control	764:770	arg1	diet					772:775	control diet	764:775	control diet supplemented with 10% oligofructose (CF)	764:816	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	43	theme	control	764:770	arg1	groups					738:743	four groups	733:743	four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF)	733:970	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	44	theme	control	746:752	arg1	diet					754:757	control diet	746:757	control diet (C)	746:761	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	44	theme	control	746:752	arg1	C					760:760	C	760:760	C	760:760	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	44	theme	control	746:752	arg1	groups					738:743	four groups	733:743	four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF)	733:970	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	11	45	theme	DNA	1739:1741	arg1	levels					1743:1748	genomic DNA levels	1731:1748	genomic DNA levels of lactobacillus spp	1731:1769	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	0	46	theme	Oligofructose	0:12	arg1	supplementation					14:28	Oligofructose supplementation	0:28	Oligofructose supplementation during pregnancy and lactation	0:59	Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups.
24502385	13	47	with	addition	1948:1955	arg1	fat					1985:1987	hydrogenated vegetable fat	1962:1987	hydrogenated vegetable fat	1962:1987	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	11	48	from	gain	1568:1571	arg1	group					1612:1616	the CF and TF group	1598:1616	group	1612:1616	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	11	48	from	gain	1568:1571	arg1	group					1638:1642	T group	1636:1642	T group	1636:1642	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	13	49	theme	hydrogenated	1962:1973	arg1	fat					1985:1987	hydrogenated vegetable fat	1962:1987	hydrogenated vegetable fat	1962:1987	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	4	50	theme	%	951:951	arg1	oligofructose					953:965	10% oligofructose	949:965	10% oligofructose (TF)	949:970	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	50	theme	%	951:951	arg1	TF					968:969	TF	968:969	TF	968:969	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	11	51	theme	spp	1767:1769	arg1	increase					1705:1712	an increase	1702:1712	an increase in serum LPS	1702:1725	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	11	51	theme	spp	1767:1769	arg1	levels					1743:1748	genomic DNA levels	1731:1748	genomic DNA levels of lactobacillus spp	1731:1769	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	11	52	from	weight	1548:1553	arg1	group					1612:1616	the CF and TF group	1598:1616	group	1612:1616	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	11	52	from	weight	1548:1553	arg1	group					1638:1642	T group	1636:1642	T group	1636:1642	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	3	53	theme	offspring	661:669	arg1	endotoxemia					611:621	endotoxemia	611:621	endotoxemia	611:621	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	3	53	theme	offspring	661:669	arg1	composition					637:647	bacterial composition	627:647	bacterial composition	627:647	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	3	53	theme	offspring	661:669	arg1	development					598:608	development	598:608	development	598:608	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	2	54	theme	intestinal	344:353	arg1	environment					355:365	the intestinal environment	340:365	the intestinal environment	340:365	On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations.
24502385	3	55	theme	diet	482:485	arg1	supplementation					487:501	the oligofructose 10% diet supplementation	460:501	the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation	460:589	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	9	56	theme	mean ± standard	1276:1290	arg1	error					1292:1296	mean ± standard error	1276:1296	mean ± standard error of the mean	1276:1308	Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures.
24502385	9	56	theme	mean ± standard	1276:1290	arg1	Data					1253:1256	Data	1253:1256	Data	1253:1256	Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures.
24502385	2	57	theme	serum	377:381	arg1	concentrations					409:422	serum lipopolysaccharides (LPS) concentrations	377:422	serum lipopolysaccharides (LPS) concentrations	377:422	On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations.
24502385	13	58	theme	offspring	2000:2008	arg1	development					2010:2020	the offspring development	1996:2020	the offspring development	1996:2020	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	7	59	theme	Serum	1100:1104	arg1	concentrations					1106:1119	Serum concentrations	1100:1119	Serum concentrations of LPS and free fatty acids (FFA)	1100:1153	Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits.
24502385	11	60	theme	serum	1717:1721	arg1	LPS					1723:1725	serum LPS	1717:1725	serum LPS	1717:1725	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	1	61	theme	pro-inflammatory	258:273	arg1	status					275:280	a pro-inflammatory status	256:280	a pro-inflammatory status in the offspring	256:297	BACKGROUND Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring.
24502385	11	62	theme	body	1543:1546	arg1	weight					1548:1553	body weight	1543:1553	body weight	1543:1553	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	3	63	theme	fat	556:558	arg1	absence					522:528	absence	522:528	absence	522:528	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	3	63	theme	fat	556:558	arg1	presence					510:517	presence	510:517	presence	510:517	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	4	64	theme	vegetable	851:859	arg1	fat					861:863	hydrogenated vegetable fat	838:863	hydrogenated vegetable fat	838:863	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	13	65	from	concentrations	2080:2093	arg1	pups					2129:2132	21d-old pups	2121:2132	21d-old pups	2121:2132	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	3	66	theme	hydrogenated	533:544	arg1	fat					556:558	hydrogenated vegetable fat	533:558	hydrogenated vegetable fat	533:558	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	11	67	theme	weight	1561:1566	arg1	gain					1568:1571	body weight gain	1556:1571	body weight gain	1556:1571	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	3	68	from	supplementation	487:501	arg1	absence					522:528	absence	522:528	absence	522:528	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	3	68	from	supplementation	487:501	arg1	presence					510:517	presence	510:517	presence	510:517	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	4	69	theme	first	687:691	arg1	day					693:695	the first day	683:695	the first day of pregnancy rats	683:713	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	1	70	theme	fatty	204:208	arg1	acids					210:214	trans fatty acids	198:214	trans fatty acids	198:214	BACKGROUND Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring.
24502385	12	71	theme	CF	1789:1790	arg1	group					1792:1796	the CF group	1785:1796	the CF group	1785:1796	on faeces of the CF group in relation to C group.
24502385	12	72	from	faeces	1775:1780	arg1	relation					1801:1808	relation	1801:1808	relation to C group	1801:1819	on faeces of the CF group in relation to C group.
24502385	1	73	dep	BACKGROUND	146:155	arg1	showed					172:177	showed	172:177	showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring	172:297	BACKGROUND Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring.
24502385	8	74	theme	DNA	1198:1200	arg1	present					1202:1208	Bacterial DNA present	1188:1208	Bacterial DNA present in faeces	1188:1218	Bacterial DNA present in faeces was determined by real-time PCR.
24502385	7	75	theme	fatty	1137:1141	arg1	FFA					1150:1152	FFA	1150:1152	FFA	1150:1152	Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits.
24502385	7	75	theme	fatty	1137:1141	arg1	acids					1143:1147	free fatty acids	1132:1147	free fatty acids (FFA)	1132:1153	Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits.
24502385	13	76	theme	serum	2074:2078	arg1	concentrations					2080:2093	the serum concentrations	2070:2093	the serum concentrations of lipopolysaccharides in 21d-old pups	2070:2132	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	13	77	with	supplementation	1859:1873	arg1	oligofructose					1887:1899	oligofructose	1887:1899	oligofructose	1887:1899	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	13	77	with	supplementation	1859:1873	arg1	%					1882:1882	10%	1880:1882	10% of oligofructose	1880:1899	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	4	78	theme	vegetable	917:925	arg1	fat					927:929	hydrogenated vegetable fat	904:929	hydrogenated vegetable fat supplemented with 10% oligofructose (TF)	904:970	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	7	79	theme	LPS	1124:1126	arg1	concentrations					1106:1119	Serum concentrations	1100:1119	Serum concentrations of LPS and free fatty acids (FFA)	1100:1153	Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits.
24502385	4	80	theme	rats	710:713	arg1	day					693:695	the first day	683:695	the first day of pregnancy rats	683:713	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	0	81	theme	21-d-old	131:138	arg1	pups					140:143	21-d-old pups	131:143	21-d-old pups	131:143	Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups.
24502385	1	82	theme	acids	210:214	arg1	intake					188:193	the intake	184:193	the intake of trans fatty acids during pregnancy and lactation	184:245	BACKGROUND Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring.
24502385	11	83	from	FFA	1591:1593	arg1	group					1612:1616	the CF and TF group	1598:1616	group	1612:1616	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	11	83	from	FFA	1591:1593	arg1	group					1638:1642	T group	1636:1642	T group	1636:1642	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	11	84	theme	lactobacillus	1753:1765	arg1	spp					1767:1769	lactobacillus spp	1753:1769	lactobacillus spp	1753:1769	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	3	85	dep	presence	510:517	arg1	the					506:508	the	506:508	the	506:508	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	11	86	from	increase	1705:1712	arg1	LPS					1723:1725	serum LPS	1717:1725	serum LPS	1717:1725	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	2	87	theme	lipopolysaccharides	383:401	arg1	concentrations					409:422	serum lipopolysaccharides (LPS) concentrations	377:422	serum lipopolysaccharides (LPS) concentrations	377:422	On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations.
24502385	11	88	theme	serum	1585:1589	arg1	FFA					1591:1593	serum FFA	1585:1593	serum FFA	1585:1593	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	3	89	theme	%	480:480	arg1	supplementation					487:501	the oligofructose 10% diet supplementation	460:501	the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation	460:589	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	3	90	theme	21-d-old	652:659	arg1	offspring					661:669	21-d-old offspring	652:669	21-d-old offspring	652:669	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	4	91	theme	%	797:797	arg1	oligofructose					799:811	10% oligofructose	795:811	10% oligofructose (CF)	795:816	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	4	91	theme	%	797:797	arg1	CF					814:815	CF	814:815	CF	814:815	METHODS On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF).
24502385	3	92	theme	oligofructose	464:476	arg1	supplementation					487:501	the oligofructose 10% diet supplementation	460:501	the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation	460:589	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	0	93	theme	offspring	69:77	arg1	development					79:89	offspring development	69:89	offspring development	69:89	Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups.
24502385	7	94	theme	specific	1173:1180	arg1	kits					1182:1185	specific kits	1173:1185	specific kits	1173:1185	Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits.
24502385	11	95	theme	CF	1602:1603	arg1	group					1612:1616	the CF and TF group	1598:1616	group	1612:1616	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	13	96	theme	bacterial	2034:2042	arg1	composition					2044:2054	the bacterial composition	2030:2054	the bacterial composition	2030:2054	CONCLUSION In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
24502385	11	97	dep	RESULTS	1439:1445	arg1	observed					1450:1457	observed	1450:1457	observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp	1450:1769	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
24502385	3	98	theme	supplementation	487:501	arg1	effect					450:455	the effect	446:455	the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring	446:669	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	2	99	theme	LPS	404:406	arg1	concentrations					409:422	serum lipopolysaccharides (LPS) concentrations	377:422	serum lipopolysaccharides (LPS) concentrations	377:422	On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations.
24502385	3	100	from	effect	450:455	arg1	composition					637:647	bacterial composition	627:647	bacterial composition	627:647	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	3	100	from	effect	450:455	arg1	endotoxemia					611:621	endotoxemia	611:621	endotoxemia	611:621	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	3	100	from	effect	450:455	arg1	absence					522:528	absence	522:528	absence	522:528	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	3	100	from	effect	450:455	arg1	development					598:608	development	598:608	development	598:608	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	3	100	from	effect	450:455	arg1	presence					510:517	presence	510:517	presence	510:517	This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.
24502385	11	101	theme	genomic	1731:1737	arg1	levels					1743:1748	genomic DNA levels	1731:1748	genomic DNA levels of lactobacillus spp	1731:1769	RESULTS We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp.
27617769	6	0	theme	FRAP	1746:1749	arg1	value					1751:1755	it's FRAP value	1741:1755	it's FRAP value	1741:1755	The ORAC value of 400W sonicated pectin increased five hold above the native pectin, while it's FRAP value was almost three fold higher than native pectin.
27617769	4	1	theme	random	860:865	arg1	scission					874:881	random pectin scission	860:881	random pectin scission	860:881	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	2	2	from	dispersions	288:298	arg1	water					342:346	deionized water	332:346	deionized water	332:346	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	3	3	theme	sonicated	675:683	arg1	products					692:699	sonicated pectin products	675:699	sonicated pectin products	675:699	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	5	4	theme	treated	1634:1640	arg1	pectin					1642:1647	native and 100W treated pectin	1618:1647	native and 100W treated pectin	1618:1647	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	5	5	theme	highest	1501:1507	arg1	4mg/mL					1530:1535	highest pectin concentration 4mg/mL	1501:1535	highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin	1501:1647	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	4	6	theme	clear	828:832	arg1	trend					834:838	clear trend	828:838	clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin	828:1360	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	2	7	theme	potato	274:279	arg1	dispersions					288:298	Sweet potato pectin dispersions	268:298	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water	268:346	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	1	8	theme	potato	148:153	arg1	weight					172:177	sweet potato pectin molecular weight	142:177	sweet potato pectin molecular weight	142:177	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	6	9	theme	native	1791:1796	arg1	pectin					1798:1803	native pectin	1791:1803	native pectin	1791:1803	The ORAC value of 400W sonicated pectin increased five hold above the native pectin, while it's FRAP value was almost three fold higher than native pectin.
27617769	5	10	theme	high	1587:1590	arg1	activity					1604:1611	substantially high antioxidant activity	1573:1611	substantially high antioxidant activity than native and 100W treated pectin	1573:1647	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	3	11	theme	products	692:699	arg1	content					614:620	galacturonic acid content	596:620	galacturonic acid content	596:620	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	3	11	theme	products	692:699	arg1	weight					588:593	subsequently the molecular weight	561:593	subsequently the molecular weight	561:593	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	3	11	theme	products	692:699	arg1	activity					663:670	antioxidant activity	651:670	antioxidant activity of sonicated pectin products	651:699	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	3	11	theme	products	692:699	arg1	levels					553:558	duty cycle levels	542:558	duty cycle levels	542:558	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	3	11	theme	products	692:699	arg1	degree					623:628	degree	623:628	degree of methoxylation	623:645	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	1	12	theme	molecular	162:170	arg1	weight					172:177	sweet potato pectin molecular weight	142:177	sweet potato pectin molecular weight	142:177	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	4	13	theme	galacturonic	1072:1083	arg1	content					1090:1096	galacturonic acid content	1072:1096	galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%	1072:1163	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	3	14	theme	cycle	547:551	arg1	levels					553:558	duty cycle levels	542:558	duty cycle levels	542:558	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	4	15	from	increase	1060:1067	arg1	%					1163:1163	92.0±2.7%	1155:1163	92.0±2.7%	1155:1163	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	15	from	increase	1060:1067	arg1	content					1090:1096	galacturonic acid content	1072:1096	galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%	1072:1163	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	15	from	increase	1060:1067	arg1	%					1111:1111	72.0±1.2%	1103:1111	72.0±1.2% in native pectin to between 85.0±3.2%	1103:1149	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	1	16	theme	ultrasound	90:99	arg1	power					116:120	power	116:120	power	116:120	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	16	theme	ultrasound	90:99	arg1	cycle					132:136	duty cycle	127:136	duty cycle	127:136	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	16	theme	ultrasound	90:99	arg1	time					110:113	time	110:113	time	110:113	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	16	theme	ultrasound	90:99	arg1	factors					101:107	ultrasound factors	90:107	ultrasound factors (time, power, and duty cycle)	90:137	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	2	17	theme	concentration	430:442	arg1	effect					393:398	effect	393:398	effect of sonication time and pectin concentration on sonolysis	393:455	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	0	18	theme	antioxidant	54:64	arg1	activity					66:73	its antioxidant activity	50:73	its antioxidant activity	50:73	Ultrasonic degradation of sweet potato pectin and its antioxidant activity.
27617769	4	19	from	100W	1028:1031	arg1	power					1017:1021	increased sonication power	996:1021	increased sonication power from 100W to 400W	996:1039	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	20	theme	threefold	948:956	arg1	reduction					958:966	approximately threefold reduction	934:966	approximately threefold reduction in pectin molecular weight	934:993	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	6	21	theme	sonicated	1673:1681	arg1	pectin					1683:1688	400W sonicated pectin	1668:1688	400W sonicated pectin	1668:1688	The ORAC value of 400W sonicated pectin increased five hold above the native pectin, while it's FRAP value was almost three fold higher than native pectin.
27617769	7	22	theme	primary	1847:1853	arg1	structure					1855:1863	pectin primary structure	1840:1863	pectin primary structure	1840:1863	However, ultrasound did not alter pectin primary structure as showed by FTIR and HPAEC results.
27617769	6	23	theme	ORAC	1654:1657	arg1	value					1659:1663	The ORAC value	1650:1663	The ORAC value of 400W sonicated pectin	1650:1688	The ORAC value of 400W sonicated pectin increased five hold above the native pectin, while it's FRAP value was almost three fold higher than native pectin.
27617769	4	24	theme	methoxylation	1180:1192	arg1	degree					1170:1175	the degree	1166:1175	the degree of methoxylation	1166:1192	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	25	theme	ultrasound	740:749	arg1	treatment					751:759	ultrasound treatment	740:759	ultrasound treatment	740:759	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	8	26	theme	effective	1947:1955	arg1	process					1967:1973	effective and green process	1947:1973	effective and green process	1947:1973	The results indicated that ultrasound offers effective and green process for pectin transformation and creation of antioxidant potent pectin products.
27617769	5	27	theme	antioxidant	1402:1412	arg1	capacity					1414:1421	increased antioxidant capacity	1392:1421	increased antioxidant capacity	1392:1421	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	2	28	theme	deionized	332:340	arg1	water					342:346	deionized water	332:346	deionized water	332:346	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	0	29	theme	Ultrasonic	0:9	arg1	degradation					11:21	Ultrasonic degradation	0:21	Ultrasonic degradation of sweet potato pectin	0:44	Ultrasonic degradation of sweet potato pectin and its antioxidant activity.
27617769	1	30	theme	antioxidant	229:239	arg1	activity					241:248	antioxidant activity	229:248	antioxidant activity	229:248	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	8	31	theme	pectin	1979:1984	arg1	transformation					1986:1999	pectin transformation	1979:1999	pectin transformation	1979:1999	The results indicated that ultrasound offers effective and green process for pectin transformation and creation of antioxidant potent pectin products.
27617769	4	32	theme	sonication	1006:1015	arg1	power					1017:1021	increased sonication power	996:1021	increased sonication power from 100W to 400W	996:1039	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	5	33	theme	higher	1467:1472	arg1	ORAC					1474:1477	higher ORAC and FRAP values	1467:1493	ORAC	1474:1477	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	5	34	theme	antioxidant	1592:1602	arg1	activity					1604:1611	substantially high antioxidant activity	1573:1611	substantially high antioxidant activity than native and 100W treated pectin	1573:1647	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	1	35	theme	factors	101:107	arg1	effect					80:85	The effect	76:85	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity	76:248	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	2	36	theme	sonication	403:412	arg1	time					414:417	sonication time	403:417	sonication time	403:417	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	3	37	theme	galacturonic	596:607	arg1	content					614:620	galacturonic acid content	596:620	galacturonic acid content	596:620	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	0	38	theme	potato	32:37	arg1	pectin					39:44	sweet potato pectin	26:44	sweet potato pectin	26:44	Ultrasonic degradation of sweet potato pectin and its antioxidant activity.
27617769	4	39	theme	molecular	978:986	arg1	weight					988:993	pectin molecular weight	971:993	pectin molecular weight	971:993	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	5	40	theme	400W	1438:1441	arg1	pectin					1453:1458	400W sonicated pectin	1438:1458	400W sonicated pectin	1438:1458	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	5	41	from	4mg/mL	1530:1535	arg1	0.8mg/ml					1549:1556	0.8mg/ml	1549:1556	0.8mg/ml	1549:1556	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	5	41	from	4mg/mL	1530:1535	arg1	ORAC					1540:1543	ORAC	1540:1543	ORAC	1540:1543	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	8	42	theme	potent	2029:2034	arg1	products					2043:2050	antioxidant potent pectin products	2017:2050	antioxidant potent pectin products	2017:2050	The results indicated that ultrasound offers effective and green process for pectin transformation and creation of antioxidant potent pectin products.
27617769	1	43	theme	neutral	180:186	arg1	composition					194:204	neutral sugar composition	180:204	neutral sugar composition	180:204	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	8	44	theme	products	2043:2050	arg1	creation					2005:2012	creation	2005:2012	creation	2005:2012	The results indicated that ultrasound offers effective and green process for pectin transformation and creation of antioxidant potent pectin products.
27617769	8	44	theme	products	2043:2050	arg1	transformation					1986:1999	pectin transformation	1979:1999	pectin transformation	1979:1999	The results indicated that ultrasound offers effective and green process for pectin transformation and creation of antioxidant potent pectin products.
27617769	4	45	theme	significant	1048:1058	arg1	increase					1060:1067	significant increase	1048:1067	significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%	1048:1163	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	5	46	theme	pectin	1509:1514	arg1	4mg/mL					1530:1535	highest pectin concentration 4mg/mL	1501:1535	highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin	1501:1647	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	4	47	theme	molecular	776:784	arg1	weight					786:791	pectin molecular weight	769:791	pectin molecular weight	769:791	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	5	48	from	0.8mg/ml	1549:1556	arg1	FRAP					1561:1564	FRAP	1561:1564	FRAP giving substantially high antioxidant activity than native and 100W treated pectin	1561:1647	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	3	49	theme	antioxidant	651:661	arg1	activity					663:670	antioxidant activity	651:670	antioxidant activity of sonicated pectin products	651:699	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	1	50	theme	pectin	207:212	arg1	structure					214:222	pectin structure	207:222	pectin structure	207:222	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	5	51	from	ORAC	1540:1543	arg1	FRAP					1561:1564	FRAP	1561:1564	FRAP giving substantially high antioxidant activity than native and 100W treated pectin	1561:1647	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	7	52	dep	FTIR	1878:1881	arg1	results					1893:1899	results	1893:1899	results	1893:1899	However, ultrasound did not alter pectin primary structure as showed by FTIR and HPAEC results.
27617769	3	53	theme	methoxylation	633:645	arg1	content					614:620	galacturonic acid content	596:620	galacturonic acid content	596:620	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	3	53	theme	methoxylation	633:645	arg1	weight					588:593	subsequently the molecular weight	561:593	subsequently the molecular weight	561:593	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	3	53	theme	methoxylation	633:645	arg1	activity					663:670	antioxidant activity	651:670	antioxidant activity of sonicated pectin products	651:699	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	3	53	theme	methoxylation	633:645	arg1	levels					553:558	duty cycle levels	542:558	duty cycle levels	542:558	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	3	53	theme	methoxylation	633:645	arg1	degree					623:628	degree	623:628	degree of methoxylation	623:645	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	3	54	theme	pectin	685:690	arg1	products					692:699	sonicated pectin products	675:699	sonicated pectin products	675:699	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	3	55	theme	further	462:468	arg1	experiments					470:480	further experiments	462:480	further experiments	462:480	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	4	56	from	%	1111:1111	arg1	pectin					1123:1128	native pectin	1116:1128	native pectin to between 85.0±3.2%	1116:1149	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	56	from	%	1111:1111	arg1	increase					1060:1067	significant increase	1048:1067	significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%	1048:1163	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	56	from	%	1111:1111	arg1	content					1090:1096	galacturonic acid content	1072:1096	galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%	1072:1163	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	2	57	theme	pectin	281:286	arg1	dispersions					288:298	Sweet potato pectin dispersions	268:298	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water	268:346	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	1	58	theme	sweet	142:146	arg1	weight					172:177	sweet potato pectin molecular weight	142:177	sweet potato pectin molecular weight	142:177	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	4	59	dep	%	920:920	arg1	to					915:916	to	915:916	to	915:916	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	2	60	theme	Sweet	268:272	arg1	dispersions					288:298	Sweet potato pectin dispersions	268:298	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water	268:346	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	1	61	theme	pectin	155:160	arg1	weight					172:177	sweet potato pectin molecular weight	142:177	sweet potato pectin molecular weight	142:177	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	3	62	theme	ultrasonic	521:530	arg1	power					532:536	ultrasonic power	521:536	ultrasonic power	521:536	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	4	63	theme	duty	895:898	arg1	cycle					900:904	duty cycle	895:904	duty cycle	895:904	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	64	theme	acid	1085:1088	arg1	content					1090:1096	galacturonic acid content	1072:1096	galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%	1072:1163	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	65	from	decrease	1303:1310	arg1	rhamnose					1315:1322	rhamnose	1315:1322	rhamnose consistent with debranching of pectin	1315:1360	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	66	theme	consistent	1324:1333	arg1	rhamnose					1315:1322	rhamnose	1315:1322	rhamnose consistent with debranching of pectin	1315:1360	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	3	67	theme	duty	542:545	arg1	levels					553:558	duty cycle levels	542:558	duty cycle levels	542:558	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	4	68	from	galactose	1289:1297	arg1	rhamnose					1315:1322	rhamnose	1315:1322	rhamnose consistent with debranching of pectin	1315:1360	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	69	theme	pectin	867:872	arg1	scission					874:881	random pectin scission	860:881	random pectin scission	860:881	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	5	70	dep	lead	1384:1387	arg1	having					1460:1465	having	1460:1465	having	1460:1465	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	4	71	theme	native	1116:1121	arg1	pectin					1123:1128	native pectin	1116:1128	native pectin to between 85.0±3.2%	1116:1149	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	2	72	theme	pectin	423:428	arg1	concentration					430:442	pectin concentration	423:442	pectin concentration	423:442	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	6	73	theme	pectin	1683:1688	arg1	value					1659:1663	The ORAC value	1650:1663	The ORAC value of 400W sonicated pectin	1650:1688	The ORAC value of 400W sonicated pectin increased five hold above the native pectin, while it's FRAP value was almost three fold higher than native pectin.
27617769	1	74	dep	factors	101:107	arg1	factors					101:107	ultrasound factors	90:107	ultrasound factors (time, power, and duty cycle)	90:137	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	74	dep	factors	101:107	arg1	power					116:120	power	116:120	power	116:120	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	74	dep	factors	101:107	arg1	time					110:113	time	110:113	time	110:113	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	74	dep	factors	101:107	arg1	cycle					132:136	duty cycle	127:136	duty cycle	127:136	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	75	theme	duty	127:130	arg1	factors					101:107	ultrasound factors	90:107	ultrasound factors (time, power, and duty cycle)	90:137	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	75	theme	duty	127:130	arg1	cycle					132:136	duty cycle	127:136	duty cycle	127:136	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	2	76	theme	time	414:417	arg1	effect					393:398	effect	393:398	effect of sonication time and pectin concentration on sonolysis	393:455	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	6	77	theme	400W	1668:1671	arg1	pectin					1683:1688	400W sonicated pectin	1668:1688	400W sonicated pectin	1668:1688	The ORAC value of 400W sonicated pectin increased five hold above the native pectin, while it's FRAP value was almost three fold higher than native pectin.
27617769	7	78	theme	pectin	1840:1845	arg1	structure					1855:1863	pectin primary structure	1840:1863	pectin primary structure	1840:1863	However, ultrasound did not alter pectin primary structure as showed by FTIR and HPAEC results.
27617769	4	79	theme	%	913:913	arg1	%					920:920	20% to 80%	911:920	20% to 80% resulted in approximately threefold reduction in pectin molecular weight	911:993	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	2	80	from	effect	393:398	arg1	sonolysis					447:455	sonolysis	447:455	sonolysis	447:455	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	4	81	with	consistent	1324:1333	arg1	debranching					1340:1350	debranching	1340:1350	debranching of pectin	1340:1360	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	82	theme	pectin	1355:1360	arg1	debranching					1340:1350	debranching	1340:1350	debranching of pectin	1340:1360	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	5	83	theme	increased	1392:1400	arg1	capacity					1414:1421	increased antioxidant capacity	1392:1421	increased antioxidant capacity	1392:1421	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	5	84	theme	100W	1629:1632	arg1	pectin					1642:1647	native and 100W treated pectin	1618:1647	native and 100W treated pectin	1618:1647	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	2	85	dep	dispersions	288:298	arg1	0.0025					301:306	0.0025	301:306	0.0025	301:306	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	2	85	dep	dispersions	288:298	arg1	0.01g/mL					319:326	0.01g/mL	319:326	0.01g/mL	319:326	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	2	85	dep	dispersions	288:298	arg1	0.005					309:313	0.005	309:313	0.005	309:313	Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis.
27617769	0	86	theme	sweet	26:30	arg1	pectin					39:44	sweet potato pectin	26:44	sweet potato pectin	26:44	Ultrasonic degradation of sweet potato pectin and its antioxidant activity.
27617769	4	87	from	%	1163:1163	arg1	pectin					1123:1128	native pectin	1116:1128	native pectin to between 85.0±3.2%	1116:1149	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	87	from	%	1163:1163	arg1	increase					1060:1067	significant increase	1048:1067	significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%	1048:1163	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	87	from	%	1163:1163	arg1	content					1090:1096	galacturonic acid content	1072:1096	galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%	1072:1163	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	4	88	theme	increased	996:1004	arg1	power					1017:1021	increased sonication power	996:1021	increased sonication power from 100W to 400W	996:1039	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	6	89	theme	native	1720:1725	arg1	pectin					1727:1732	the native pectin	1716:1732	the native pectin	1716:1732	The ORAC value of 400W sonicated pectin increased five hold above the native pectin, while it's FRAP value was almost three fold higher than native pectin.
27617769	0	90	theme	pectin	39:44	arg1	degradation					11:21	Ultrasonic degradation	0:21	Ultrasonic degradation of sweet potato pectin	0:44	Ultrasonic degradation of sweet potato pectin and its antioxidant activity.
27617769	0	90	theme	pectin	39:44	arg1	activity					66:73	its antioxidant activity	50:73	its antioxidant activity	50:73	Ultrasonic degradation of sweet potato pectin and its antioxidant activity.
27617769	8	91	theme	antioxidant	2017:2027	arg1	products					2043:2050	antioxidant potent pectin products	2017:2050	antioxidant potent pectin products	2017:2050	The results indicated that ultrasound offers effective and green process for pectin transformation and creation of antioxidant potent pectin products.
27617769	5	92	theme	FRAP	1483:1486	arg1	values					1488:1493	higher ORAC and FRAP values	1467:1493	values	1488:1493	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	3	93	theme	molecular	578:586	arg1	weight					588:593	subsequently the molecular weight	561:593	subsequently the molecular weight	561:593	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	5	94	theme	native	1618:1623	arg1	pectin					1642:1647	native and 100W treated pectin	1618:1647	native and 100W treated pectin	1618:1647	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	8	95	theme	green	1961:1965	arg1	process					1967:1973	effective and green process	1947:1973	effective and green process	1947:1973	The results indicated that ultrasound offers effective and green process for pectin transformation and creation of antioxidant potent pectin products.
27617769	8	96	theme	pectin	2036:2041	arg1	products					2043:2050	antioxidant potent pectin products	2017:2050	antioxidant potent pectin products	2017:2050	The results indicated that ultrasound offers effective and green process for pectin transformation and creation of antioxidant potent pectin products.
27617769	4	97	theme	pectin	971:976	arg1	weight					988:993	pectin molecular weight	971:993	pectin molecular weight	971:993	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	5	98	theme	sonicated	1443:1451	arg1	pectin					1453:1458	400W sonicated pectin	1438:1458	400W sonicated pectin	1438:1458	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	4	99	from	reduction	958:966	arg1	weight					988:993	pectin molecular weight	971:993	pectin molecular weight	971:993	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
27617769	3	100	theme	acid	609:612	arg1	content					614:620	galacturonic acid content	596:620	galacturonic acid content	596:620	For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated.
27617769	1	101	from	effect	80:85	arg1	composition					194:204	neutral sugar composition	180:204	neutral sugar composition	180:204	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	101	from	effect	80:85	arg1	structure					214:222	pectin structure	207:222	pectin structure	207:222	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	101	from	effect	80:85	arg1	weight					172:177	sweet potato pectin molecular weight	142:177	sweet potato pectin molecular weight	142:177	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	101	from	effect	80:85	arg1	activity					241:248	antioxidant activity	229:248	antioxidant activity	229:248	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	1	102	theme	sugar	188:192	arg1	composition					194:204	neutral sugar composition	180:204	neutral sugar composition	180:204	The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated.
27617769	5	103	theme	concentration	1516:1528	arg1	4mg/mL					1530:1535	highest pectin concentration 4mg/mL	1501:1535	highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin	1501:1647	Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin.
27617769	4	104	theme	pectin	769:774	arg1	weight					786:791	pectin molecular weight	769:791	pectin molecular weight	769:791	Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin.
28837924	2	0	theme	Mn3	457:459	arg1	materials					485:493	GOx@Mn3(PO4)2 hybrid functional materials	453:493	GOx@Mn3(PO4)2 hybrid functional materials	453:493	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	2	1	contain	containing	329:338	arg2	oxidase					366:372	glucose oxidase	358:372	glucose oxidase	358:372	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	2	1	contain	containing	329:338	arg2	enzyme					350:355	a diluted enzyme	340:355	a diluted enzyme (glucose oxidase, GOx)	340:378	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	2	1	contain	containing	329:338	arg1	solution					300:307	An inorganic solution	287:307	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx)	287:378	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	6	2	theme	natural	1195:1201	arg1	hybrid					1225:1230	The self-assembled natural and artificial enzyme hybrid	1176:1230	The self-assembled natural and artificial enzyme hybrid on the μPADs	1176:1243	The self-assembled natural and artificial enzyme hybrid on the μPADs specifically detected glucose from a group of interferences, which shows great specificity using this method.
28837924	7	3	from	samples	1506:1512	arg1	range					1475:1479	the physiological range	1457:1479	the physiological range of glucose in biological samples	1457:1512	Moreover, the colorimetric signal exhibited detection limitation for glucose is 0.01mM, which lies in the physiological range of glucose in biological samples.
28837924	1	4	theme	analytical	153:162	arg1	μPAD					172:175	μPAD	172:175	μPAD	172:175	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	1	4	theme	analytical	153:162	arg1	device					164:169	A visual colorimetric microfluidic paper-based analytical device	106:169	A visual colorimetric microfluidic paper-based analytical device (μPAD)	106:176	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	7	5	from	glucose	1484:1490	arg1	samples					1506:1512	biological samples	1495:1512	biological samples	1495:1512	Moreover, the colorimetric signal exhibited detection limitation for glucose is 0.01mM, which lies in the physiological range of glucose in biological samples.
28837924	1	6	theme	nanomaterials	252:264	arg1	synthesis					215:223	the direct synthesis	204:223	the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix	204:284	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	2	7	theme	@	456:456	arg1	materials					485:493	GOx@Mn3(PO4)2 hybrid functional materials	453:493	GOx@Mn3(PO4)2 hybrid functional materials	453:493	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	6	8	theme	self-assembled	1180:1193	arg1	hybrid					1225:1230	The self-assembled natural and artificial enzyme hybrid	1176:1230	The self-assembled natural and artificial enzyme hybrid on the μPADs	1176:1243	The self-assembled natural and artificial enzyme hybrid on the μPADs specifically detected glucose from a group of interferences, which shows great specificity using this method.
28837924	5	9	theme	pipetting	1104:1112	arg1	accuracy					1078:1085	accuracy	1078:1085	accuracy	1078:1085	The sensing material loading was highly reproducible because of the accuracy and stability of pipetting, which eventually contributed to the reliability of the μPAD.
28837924	5	9	theme	pipetting	1104:1112	arg1	stability					1091:1099	stability	1091:1099	stability	1091:1099	The sensing material loading was highly reproducible because of the accuracy and stability of pipetting, which eventually contributed to the reliability of the μPAD.
28837924	2	10	theme	functional	474:483	arg1	materials					485:493	GOx@Mn3(PO4)2 hybrid functional materials	453:493	GOx@Mn3(PO4)2 hybrid functional materials	453:493	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	5	11	dep	accuracy	1078:1085	arg1	the					1074:1076	the	1074:1076	the	1074:1076	The sensing material loading was highly reproducible because of the accuracy and stability of pipetting, which eventually contributed to the reliability of the μPAD.
28837924	2	12	theme	inorganic	290:298	arg1	solution					300:307	An inorganic solution	287:307	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx)	287:378	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	4	13	theme	dry	964:966	arg1	process					968:974	the dry process	960:974	the dry process	960:974	This new approach for the in situ growth of an enzyme-inorganic hybrid on a paper matrix eliminates centrifugation and the dry process by casting the solution on paper.
28837924	2	14	theme	hybrid	467:472	arg1	materials					485:493	GOx@Mn3(PO4)2 hybrid functional materials	453:493	GOx@Mn3(PO4)2 hybrid functional materials	453:493	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	4	15	theme	paper	917:921	arg1	matrix					923:928	a paper matrix	915:928	a paper matrix	915:928	This new approach for the in situ growth of an enzyme-inorganic hybrid on a paper matrix eliminates centrifugation and the dry process by casting the solution on paper.
28837924	7	16	theme	detection	1399:1407	arg1	limitation					1409:1418	detection limitation	1399:1418	detection limitation for glucose	1399:1430	Moreover, the colorimetric signal exhibited detection limitation for glucose is 0.01mM, which lies in the physiological range of glucose in biological samples.
28837924	3	17	from	presence	574:581	arg1	fiber					622:626	the paper fiber	612:626	the paper fiber	612:626	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	1	18	theme	paper	273:277	arg1	matrix					279:284	the paper matrix	269:284	the paper matrix	269:284	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	0	19	theme	glucose	97:103	arg1	detection					84:92	the visual detection	73:92	the visual detection of glucose	73:103	Efficient in situ growth of enzyme-inorganic hybrids on paper strips for the visual detection of glucose.
28837924	3	20	theme	hybrid	656:661	arg1	Mn3					639:641	the Mn3(PO4)2	635:647	the Mn3(PO4)2 of the hybrid	635:661	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	2	21	dep	oxidase	366:372	arg1	GOx					375:377	GOx	375:377	GOx	375:377	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	3	22	theme	enzyme	822:827	arg1	modulation					829:838	enzyme modulation	822:838	enzyme modulation	822:838	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	4	23	from	approach	850:857	arg1	matrix					923:928	a paper matrix	915:928	a paper matrix	915:928	This new approach for the in situ growth of an enzyme-inorganic hybrid on a paper matrix eliminates centrifugation and the dry process by casting the solution on paper.
28837924	2	24	theme	GOx	453:455	arg1	materials					485:493	GOx@Mn3(PO4)2 hybrid functional materials	453:493	GOx@Mn3(PO4)2 hybrid functional materials	453:493	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	2	25	theme	KH2PO4	322:327	arg1	solution					300:307	An inorganic solution	287:307	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx)	287:378	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	4	26	theme	in	867:868	arg1	growth					875:880	the in situ growth	863:880	the in situ growth of an enzyme-inorganic hybrid	863:910	This new approach for the in situ growth of an enzyme-inorganic hybrid on a paper matrix eliminates centrifugation and the dry process by casting the solution on paper.
28837924	0	27	theme	in	10:11	arg1	growth					18:23	Efficient in situ growth	0:23	Efficient in situ growth of enzyme-inorganic hybrids on paper	0:60	Efficient in situ growth of enzyme-inorganic hybrids on paper strips for the visual detection of glucose.
28837924	2	28	dep	in	435:436	arg1	situ					438:441	situ	438:441	situ	438:441	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	3	29	theme	morphology	524:533	arg1	characterization					500:515	The characterization	496:515	The characterization of the morphology and chemical composition	496:558	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	6	30	theme	enzyme	1218:1223	arg1	hybrid					1225:1230	The self-assembled natural and artificial enzyme hybrid	1176:1230	The self-assembled natural and artificial enzyme hybrid on the μPADs	1176:1243	The self-assembled natural and artificial enzyme hybrid on the μPADs specifically detected glucose from a group of interferences, which shows great specificity using this method.
28837924	0	31	theme	Efficient	0:8	arg1	growth					18:23	Efficient in situ growth	0:23	Efficient in situ growth of enzyme-inorganic hybrids on paper	0:60	Efficient in situ growth of enzyme-inorganic hybrids on paper strips for the visual detection of glucose.
28837924	7	32	theme	colorimetric	1369:1380	arg1	0.01mM					1435:1440	0.01mM	1435:1440	0.01mM	1435:1440	Moreover, the colorimetric signal exhibited detection limitation for glucose is 0.01mM, which lies in the physiological range of glucose in biological samples.
28837924	7	32	theme	colorimetric	1369:1380	arg1	signal					1382:1387	the colorimetric signal	1365:1387	the colorimetric signal exhibited detection limitation for glucose	1365:1430	Moreover, the colorimetric signal exhibited detection limitation for glucose is 0.01mM, which lies in the physiological range of glucose in biological samples.
28837924	3	33	theme	higher	714:719	arg1	activity					747:754	a higher peroxidase-like catalytic activity	712:754	a higher peroxidase-like catalytic activity	712:754	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	2	34	theme	MnSO4	312:316	arg1	solution					300:307	An inorganic solution	287:307	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx)	287:378	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	2	35	theme	glucose	358:364	arg1	enzyme					350:355	a diluted enzyme	340:355	a diluted enzyme (glucose oxidase, GOx)	340:378	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	2	35	theme	glucose	358:364	arg1	oxidase					366:372	glucose oxidase	358:372	glucose oxidase	358:372	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	4	36	dep	in	867:868	arg1	situ					870:873	situ	870:873	situ	870:873	This new approach for the in situ growth of an enzyme-inorganic hybrid on a paper matrix eliminates centrifugation and the dry process by casting the solution on paper.
28837924	7	37	theme	physiological	1461:1473	arg1	range					1475:1479	the physiological range	1457:1479	the physiological range of glucose in biological samples	1457:1512	Moreover, the colorimetric signal exhibited detection limitation for glucose is 0.01mM, which lies in the physiological range of glucose in biological samples.
28837924	7	38	theme	biological	1495:1504	arg1	samples					1506:1512	biological samples	1495:1512	biological samples	1495:1512	Moreover, the colorimetric signal exhibited detection limitation for glucose is 0.01mM, which lies in the physiological range of glucose in biological samples.
28837924	2	39	theme	cellulose	411:419	arg1	paper					421:425	cellulose paper	411:425	cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials	411:493	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	5	40	theme	material	1022:1029	arg1	loading					1031:1037	The sensing material loading	1010:1037	The sensing material loading	1010:1037	The sensing material loading was highly reproducible because of the accuracy and stability of pipetting, which eventually contributed to the reliability of the μPAD.
28837924	5	40	theme	material	1022:1029	arg1	reproducible					1050:1061	reproducible	1050:1061	reproducible	1050:1061	The sensing material loading was highly reproducible because of the accuracy and stability of pipetting, which eventually contributed to the reliability of the μPAD.
28837924	6	41	theme	great	1318:1322	arg1	specificity					1324:1334	great specificity	1318:1334	great specificity using this method	1318:1352	The self-assembled natural and artificial enzyme hybrid on the μPADs specifically detected glucose from a group of interferences, which shows great specificity using this method.
28837924	0	42	theme	enzyme-inorganic	28:43	arg1	hybrids					45:51	enzyme-inorganic hybrids	28:51	enzyme-inorganic hybrids	28:51	Efficient in situ growth of enzyme-inorganic hybrids on paper strips for the visual detection of glucose.
28837924	4	43	theme	new	846:848	arg1	approach					850:857	This new approach	841:857	This new approach for the in situ growth of an enzyme-inorganic hybrid on a paper matrix	841:928	This new approach for the in situ growth of an enzyme-inorganic hybrid on a paper matrix eliminates centrifugation and the dry process by casting the solution on paper.
28837924	3	44	theme	paper	616:620	arg1	fiber					622:626	the paper fiber	612:626	the paper fiber	612:626	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	3	45	theme	Mn3	775:777	arg1	crystal					785:791	the Mn3(PO4)2 crystal	771:791	the Mn3(PO4)2 crystal that was synthesized without enzyme modulation	771:838	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	4	46	theme	enzyme-inorganic	888:903	arg1	hybrid					905:910	an enzyme-inorganic hybrid	885:910	an enzyme-inorganic hybrid	885:910	This new approach for the in situ growth of an enzyme-inorganic hybrid on a paper matrix eliminates centrifugation and the dry process by casting the solution on paper.
28837924	2	47	theme	materials	485:493	arg1	growth					443:448	the in situ growth	431:448	the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials	431:493	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	3	48	theme	roots	603:607	arg1	presence					574:581	the presence	570:581	the presence of hybrid materials roots in the paper fiber	570:626	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	7	49	theme	glucose	1484:1490	arg1	range					1475:1479	the physiological range	1457:1479	the physiological range of glucose in biological samples	1457:1512	Moreover, the colorimetric signal exhibited detection limitation for glucose is 0.01mM, which lies in the physiological range of glucose in biological samples.
28837924	1	50	theme	direct	208:213	arg1	synthesis					215:223	the direct synthesis	204:223	the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix	204:284	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	7	51	from	range	1475:1479	arg1	samples					1506:1512	biological samples	1495:1512	biological samples	1495:1512	Moreover, the colorimetric signal exhibited detection limitation for glucose is 0.01mM, which lies in the physiological range of glucose in biological samples.
28837924	6	52	theme	interferences	1291:1303	arg1	interferences					1291:1303	interferences	1291:1303	interferences	1291:1303	The self-assembled natural and artificial enzyme hybrid on the μPADs specifically detected glucose from a group of interferences, which shows great specificity using this method.
28837924	6	52	theme	interferences	1291:1303	arg1	group					1282:1286	a group	1280:1286	a group	1280:1286	The self-assembled natural and artificial enzyme hybrid on the μPADs specifically detected glucose from a group of interferences, which shows great specificity using this method.
28837924	3	53	theme	chemical	539:546	arg1	composition					548:558	chemical composition	539:558	chemical composition	539:558	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	6	54	theme	artificial	1207:1216	arg1	hybrid					1225:1230	The self-assembled natural and artificial enzyme hybrid	1176:1230	The self-assembled natural and artificial enzyme hybrid on the μPADs	1176:1243	The self-assembled natural and artificial enzyme hybrid on the μPADs specifically detected glucose from a group of interferences, which shows great specificity using this method.
28837924	2	55	theme	diluted	342:348	arg1	enzyme					350:355	a diluted enzyme	340:355	a diluted enzyme (glucose oxidase, GOx)	340:378	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	2	55	theme	diluted	342:348	arg1	oxidase					366:372	glucose oxidase	358:372	glucose oxidase	358:372	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	0	56	theme	hybrids	45:51	arg1	growth					18:23	Efficient in situ growth	0:23	Efficient in situ growth of enzyme-inorganic hybrids on paper	0:60	Efficient in situ growth of enzyme-inorganic hybrids on paper strips for the visual detection of glucose.
28837924	6	57	from	hybrid	1225:1230	arg1	μPADs					1239:1243	the μPADs	1235:1243	the μPADs	1235:1243	The self-assembled natural and artificial enzyme hybrid on the μPADs specifically detected glucose from a group of interferences, which shows great specificity using this method.
28837924	5	58	theme	sensing	1014:1020	arg1	loading					1031:1037	The sensing material loading	1010:1037	The sensing material loading	1010:1037	The sensing material loading was highly reproducible because of the accuracy and stability of pipetting, which eventually contributed to the reliability of the μPAD.
28837924	5	58	theme	sensing	1014:1020	arg1	reproducible					1050:1061	reproducible	1050:1061	reproducible	1050:1061	The sensing material loading was highly reproducible because of the accuracy and stability of pipetting, which eventually contributed to the reliability of the μPAD.
28837924	3	59	theme	composition	548:558	arg1	characterization					500:515	The characterization	496:515	The characterization of the morphology and chemical composition	496:558	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	3	60	theme	enzyme	691:696	arg1	anchoring					698:706	enzyme anchoring	691:706	enzyme anchoring	691:706	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	1	61	from	synthesis	215:223	arg1	matrix					279:284	the paper matrix	269:284	the paper matrix	269:284	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	3	62	theme	hybrid	586:591	arg1	roots					603:607	hybrid materials roots	586:607	hybrid materials roots	586:607	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	1	63	theme	visual	108:113	arg1	μPAD					172:175	μPAD	172:175	μPAD	172:175	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	1	63	theme	visual	108:113	arg1	device					164:169	A visual colorimetric microfluidic paper-based analytical device	106:169	A visual colorimetric microfluidic paper-based analytical device (μPAD)	106:176	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	3	64	theme	peroxidase-like	721:735	arg1	activity					747:754	a higher peroxidase-like catalytic activity	712:754	a higher peroxidase-like catalytic activity	712:754	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	4	65	theme	hybrid	905:910	arg1	growth					875:880	the in situ growth	863:880	the in situ growth of an enzyme-inorganic hybrid	863:910	This new approach for the in situ growth of an enzyme-inorganic hybrid on a paper matrix eliminates centrifugation and the dry process by casting the solution on paper.
28837924	4	66	from	solution	991:998	arg1	paper					1003:1007	paper	1003:1007	paper	1003:1007	This new approach for the in situ growth of an enzyme-inorganic hybrid on a paper matrix eliminates centrifugation and the dry process by casting the solution on paper.
28837924	3	67	theme	materials	593:601	arg1	roots					603:607	hybrid materials roots	586:607	hybrid materials roots	586:607	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	1	68	theme	colorimetric	115:126	arg1	μPAD					172:175	μPAD	172:175	μPAD	172:175	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	1	68	theme	colorimetric	115:126	arg1	device					164:169	A visual colorimetric microfluidic paper-based analytical device	106:169	A visual colorimetric microfluidic paper-based analytical device (μPAD)	106:176	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	3	69	theme	catalytic	737:745	arg1	activity					747:754	a higher peroxidase-like catalytic activity	712:754	a higher peroxidase-like catalytic activity	712:754	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	5	70	theme	μPAD	1170:1173	arg1	reliability					1151:1161	the reliability	1147:1161	the reliability of the μPAD	1147:1173	The sensing material loading was highly reproducible because of the accuracy and stability of pipetting, which eventually contributed to the reliability of the μPAD.
28837924	0	71	dep	in	10:11	arg1	situ					13:16	situ	13:16	situ	13:16	Efficient in situ growth of enzyme-inorganic hybrids on paper strips for the visual detection of glucose.
28837924	1	72	theme	microfluidic	128:139	arg1	μPAD					172:175	μPAD	172:175	μPAD	172:175	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	1	72	theme	microfluidic	128:139	arg1	device					164:169	A visual colorimetric microfluidic paper-based analytical device	106:169	A visual colorimetric microfluidic paper-based analytical device (μPAD)	106:176	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	3	73	attach	presence	574:581	arg2	roots					603:607	hybrid materials roots	586:607	hybrid materials roots	586:607	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	3	73	attach	presence	574:581	arg1	fiber					622:626	the paper fiber	612:626	the paper fiber	612:626	The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn3(PO4)2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn3(PO4)2 crystal that was synthesized without enzyme modulation.
28837924	1	74	theme	enzyme-inorganic	228:243	arg1	nanomaterials					252:264	enzyme-inorganic hybrid nanomaterials	228:264	enzyme-inorganic hybrid nanomaterials	228:264	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	2	75	theme	in	435:436	arg1	growth					443:448	the in situ growth	431:448	the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials	431:493	An inorganic solution of MnSO4 and KH2PO4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn3(PO4)2 hybrid functional materials.
28837924	0	76	from	growth	18:23	arg1	paper					56:60	paper	56:60	paper	56:60	Efficient in situ growth of enzyme-inorganic hybrids on paper strips for the visual detection of glucose.
28837924	1	77	theme	hybrid	245:250	arg1	nanomaterials					252:264	enzyme-inorganic hybrid nanomaterials	228:264	enzyme-inorganic hybrid nanomaterials	228:264	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	1	78	theme	paper-based	141:151	arg1	μPAD					172:175	μPAD	172:175	μPAD	172:175	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	1	78	theme	paper-based	141:151	arg1	device					164:169	A visual colorimetric microfluidic paper-based analytical device	106:169	A visual colorimetric microfluidic paper-based analytical device (μPAD)	106:176	A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix.
28837924	0	79	theme	visual	77:82	arg1	detection					84:92	the visual detection	73:92	the visual detection of glucose	73:103	Efficient in situ growth of enzyme-inorganic hybrids on paper strips for the visual detection of glucose.
27516305	1	0	theme	tissue	158:163	arg1	engineering					165:175	bone tissue engineering	153:175	bone tissue engineering	153:175	In this study, suitable scaffold materials for bone tissue engineering were successfully prepared using fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate.
27516305	4	1	theme	pore	625:628	arg1	size					630:633	pore size	625:633	pore size	625:633	The scaffold was characterized for pore size, swelling, density, degradation, mineralization, cell viability and attachment, and its morphological features were examined using scanning electron microscopy.
27516305	0	2	theme	compound	94:101	arg1	K					103:103	Ginseng compound K	86:103	Ginseng compound K	86:103	Collagen/chitosan porous bone tissue engineering composite scaffold incorporated with Ginseng compound K.
27516305	3	3	theme	ginseng	387:393	arg1	K					404:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	3	3	theme	ginseng	387:393	arg1	agent					421:425	a therapeutic agent	407:425	a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2	407:541	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	3	4	theme	therapeutic	409:419	arg1	K					404:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	3	4	theme	therapeutic	409:419	arg1	agent					421:425	a therapeutic agent	407:425	a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2	407:541	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	3	5	theme	compound	395:402	arg1	K					404:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	3	5	theme	compound	395:402	arg1	agent					421:425	a therapeutic agent	407:425	a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2	407:541	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	5	6	theme	prepared	856:863	arg1	biocompatible					878:890	biocompatible	878:890	biocompatible	878:890	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	5	6	theme	prepared	856:863	arg1	scaffold					865:872	the prepared scaffold	852:872	the prepared scaffold	852:872	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	4	7	theme	morphological	723:735	arg1	features					737:744	its morphological features	719:744	its morphological features	719:744	The scaffold was characterized for pore size, swelling, density, degradation, mineralization, cell viability and attachment, and its morphological features were examined using scanning electron microscopy.
27516305	5	8	theme	alternative	967:977	arg1	approach					979:986	an alternative approach	964:986	an alternative approach for bone regeneration	964:1008	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	5	8	theme	alternative	967:977	arg1	potential					951:959	potential	951:959	potential	951:959	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	3	9	theme	traditional	365:375	arg1	K					404:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	3	9	theme	traditional	365:375	arg1	agent					421:425	a therapeutic agent	407:425	a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2	407:541	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	4	10	theme	electron	775:782	arg1	microscopy					784:793	scanning electron microscopy	766:793	scanning electron microscopy	766:793	The scaffold was characterized for pore size, swelling, density, degradation, mineralization, cell viability and attachment, and its morphological features were examined using scanning electron microscopy.
27516305	2	11	theme	composite	295:303	arg1	scaffolds					305:313	Porous composite scaffolds	288:313	Porous composite scaffolds	288:313	Porous composite scaffolds were prepared by freeze drying method.
27516305	3	12	theme	medicinal	377:385	arg1	K					404:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	3	12	theme	medicinal	377:385	arg1	agent					421:425	a therapeutic agent	407:425	a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2	407:541	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	2	13	theme	Porous	288:293	arg1	scaffolds					305:313	Porous composite scaffolds	288:313	Porous composite scaffolds	288:313	Porous composite scaffolds were prepared by freeze drying method.
27516305	4	14	theme	cell	684:687	arg1	viability					689:697	cell viability	684:697	cell viability	684:697	The scaffold was characterized for pore size, swelling, density, degradation, mineralization, cell viability and attachment, and its morphological features were examined using scanning electron microscopy.
27516305	0	15	theme	porous	18:23	arg1	scaffold					59:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold incorporated with Ginseng compound K.
27516305	5	16	theme	in	822:823	arg1	analysis					831:838	in vitro analysis	822:838	in vitro analysis	822:838	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	2	17	theme	drying	339:344	arg1	method					346:351	freeze drying method	332:351	freeze drying method	332:351	Porous composite scaffolds were prepared by freeze drying method.
27516305	5	18	theme	MG-63	920:924	arg1	cells					926:930	MG-63 cells	920:930	MG-63 cells	920:930	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	0	19	theme	Collagen/chitosan	0:16	arg1	scaffold					59:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold incorporated with Ginseng compound K.
27516305	2	20	theme	freeze	332:337	arg1	method					346:351	freeze drying method	332:351	freeze drying method	332:351	Porous composite scaffolds were prepared by freeze drying method.
27516305	3	21	theme	osteoporosis	448:459	arg1	treatment					435:443	the treatment	431:443	the treatment of osteoporosis	431:459	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	0	22	theme	tissue	30:35	arg1	scaffold					59:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold incorporated with Ginseng compound K.
27516305	4	23	theme	scanning	766:773	arg1	microscopy					784:793	scanning electron microscopy	766:793	scanning electron microscopy	766:793	The scaffold was characterized for pore size, swelling, density, degradation, mineralization, cell viability and attachment, and its morphological features were examined using scanning electron microscopy.
27516305	5	24	theme	bone	992:995	arg1	regeneration					997:1008	bone regeneration	992:1008	bone regeneration	992:1008	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	1	25	theme	fish	210:213	arg1	scale					215:219	fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate	210:285	fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate	210:285	In this study, suitable scaffold materials for bone tissue engineering were successfully prepared using fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate.
27516305	0	26	theme	bone	25:28	arg1	scaffold					59:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold incorporated with Ginseng compound K.
27516305	5	27	dep	in	822:823	arg1	vitro					825:829	vitro	825:829	vitro	825:829	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	0	28	theme	composite	49:57	arg1	scaffold					59:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold incorporated with Ginseng compound K.
27516305	3	29	theme	protein-2	533:541	arg1	production					500:509	production	500:509	production of bone morphogenetic protein-2	500:541	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	5	30	theme	cells	926:930	arg1	growth					910:915	the growth	906:915	the growth of MG-63 cells	906:930	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	0	31	theme	engineering	37:47	arg1	scaffold					59:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold	0:66	Collagen/chitosan porous bone tissue engineering composite scaffold incorporated with Ginseng compound K.
27516305	5	32	dep	characterization	801:816	arg1	This					796:799	This	796:799	This	796:799	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	3	33	theme	morphogenetic	519:531	arg1	protein-2					533:541	bone morphogenetic protein-2	514:541	bone morphogenetic protein-2	514:541	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	1	34	theme	suitable	121:128	arg1	materials					139:147	suitable scaffold materials	121:147	suitable scaffold materials for bone tissue engineering	121:175	In this study, suitable scaffold materials for bone tissue engineering were successfully prepared using fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate.
27516305	1	35	dep	collagen	221:228	arg1	phosphate					277:285	phosphate	277:285	phosphate	277:285	In this study, suitable scaffold materials for bone tissue engineering were successfully prepared using fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate.
27516305	1	36	theme	scaffold	130:137	arg1	materials					139:147	suitable scaffold materials	121:147	suitable scaffold materials for bone tissue engineering	121:175	In this study, suitable scaffold materials for bone tissue engineering were successfully prepared using fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate.
27516305	3	37	theme	composite	570:578	arg1	scaffold					580:587	the composite scaffold	566:587	the composite scaffold	566:587	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	5	38	contain	has	947:949	arg2	approach					979:986	an alternative approach	964:986	an alternative approach for bone regeneration	964:1008	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	5	38	contain	has	947:949	arg1	biocompatible					878:890	biocompatible	878:890	biocompatible	878:890	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	5	38	contain	has	947:949	arg1	scaffold					865:872	the prepared scaffold	852:872	the prepared scaffold	852:872	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	5	38	contain	has	947:949	arg2	potential					951:959	potential	951:959	potential	951:959	This characterization and in vitro analysis showed that the prepared scaffold was biocompatible and supported the growth of MG-63 cells, and therefore has potential as an alternative approach for bone regeneration.
27516305	3	39	theme	bone	514:517	arg1	protein-2					533:541	bone morphogenetic protein-2	514:541	bone morphogenetic protein-2	514:541	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	0	40	theme	Ginseng	86:92	arg1	K					103:103	Ginseng compound K	86:103	Ginseng compound K	86:103	Collagen/chitosan porous bone tissue engineering composite scaffold incorporated with Ginseng compound K.
27516305	3	41	theme	Korean	358:363	arg1	K					404:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K	354:404	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	3	41	theme	Korean	358:363	arg1	agent					421:425	a therapeutic agent	407:425	a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2	407:541	The Korean traditional medicinal ginseng compound K, a therapeutic agent for the treatment of osteoporosis that reduces inflammation and enhances production of bone morphogenetic protein-2, was incorporated into the composite scaffold.
27516305	1	42	dep	scale	215:219	arg1	chitosan					247:254	chitosan	247:254	chitosan	247:254	In this study, suitable scaffold materials for bone tissue engineering were successfully prepared using fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate.
27516305	1	42	dep	scale	215:219	arg1	beta-tricalcium					261:275	beta-tricalcium	261:275	beta-tricalcium	261:275	In this study, suitable scaffold materials for bone tissue engineering were successfully prepared using fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate.
27516305	1	42	dep	scale	215:219	arg1	hydroxyapatite					231:244	hydroxyapatite	231:244	hydroxyapatite	231:244	In this study, suitable scaffold materials for bone tissue engineering were successfully prepared using fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate.
27516305	1	42	dep	scale	215:219	arg1	collagen					221:228	collagen	221:228	collagen	221:228	In this study, suitable scaffold materials for bone tissue engineering were successfully prepared using fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate.
27516305	1	43	theme	bone	153:156	arg1	engineering					165:175	bone tissue engineering	153:175	bone tissue engineering	153:175	In this study, suitable scaffold materials for bone tissue engineering were successfully prepared using fish scale collagen, hydroxyapatite, chitosan, and beta-tricalcium phosphate.
28962762	1	0	theme	therapeutic	152:162	arg1	disadvantages					164:176	the therapeutic disadvantages	148:176	the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP)	148:224	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	0	1	theme	gold	88:91	arg1	nanoparticles					93:105	gold nanoparticles	88:105	gold nanoparticles	88:105	Construction of 6-thioguanine and 6-mercaptopurine carriers based on βcyclodextrins and gold nanoparticles.
28962762	3	2	theme	complexes	655:663	arg1	formation					638:646	The formation	634:646	The formation of the complexes	634:663	The formation of the complexes was confirmed using powder X-ray diffraction, thermogravimetric analysis and one and two-dimensional NMR.
28962762	2	3	theme	due	574:576	arg1	transport					564:572	a site-specific transport	548:572	a site-specific transport due to their nanometric dimensions, among other advantages	548:631	This modification increased their solubility and improved their stability, betting by a site-specific transport due to their nanometric dimensions, among other advantages.
28962762	4	4	theme	stable	858:863	arg1	species					876:882	the more stable tautomeric species	849:882	the more stable tautomeric species of TG and MP	849:895	A theoretical study using DFT and molecular modelling was conducted to obtain the more stable tautomeric species of TG and MP in solution and confirm the proposed inclusion geometries.
28962762	5	5	theme	AuNPs	974:978	arg1	deposition					960:969	The deposition	956:969	The deposition of AuNPs onto βCD-TG and βCD-MP via sputtering	956:1016	The deposition of AuNPs onto βCD-TG and βCD-MP via sputtering was confirmed by UV-vis spectroscopy.
28962762	4	6	theme	tautomeric	865:874	arg1	species					876:882	the more stable tautomeric species	849:882	the more stable tautomeric species of TG and MP	849:895	A theoretical study using DFT and molecular modelling was conducted to obtain the more stable tautomeric species of TG and MP in solution and confirm the proposed inclusion geometries.
28962762	6	7	theme	deposited	1156:1164	arg1	AuNPs					1166:1170	the deposited AuNPs	1152:1170	the deposited AuNPs	1152:1170	Subsequently, the ternary systems were characterized by TEM, FE-SEM and EDX to directly observe the deposited AuNPs and evaluate their sizes, size dispersion, and composition.
28962762	1	8	dep	systems	422:428	arg1	systems					422:428	the ternary systems	410:428	the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs	410:459	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	8	dep	systems	422:428	arg1	βCD-MP-AuNPs					448:459	βCD-MP-AuNPs	448:459	βCD-MP-AuNPs	448:459	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	8	dep	systems	422:428	arg1	βCD-TG-AuNPs					431:442	βCD-TG-AuNPs	431:442	βCD-TG-AuNPs	431:442	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	2	9	theme	site-specific	550:562	arg1	transport					564:572	a site-specific transport	548:572	a site-specific transport due to their nanometric dimensions, among other advantages	548:631	This modification increased their solubility and improved their stability, betting by a site-specific transport due to their nanometric dimensions, among other advantages.
28962762	2	10	theme	other	616:620	arg1	advantages					622:631	other advantages	616:631	other advantages	616:631	This modification increased their solubility and improved their stability, betting by a site-specific transport due to their nanometric dimensions, among other advantages.
28962762	6	11	theme	ternary	1074:1080	arg1	systems					1082:1088	the ternary systems	1070:1088	the ternary systems	1070:1088	Subsequently, the ternary systems were characterized by TEM, FE-SEM and EDX to directly observe the deposited AuNPs and evaluate their sizes, size dispersion, and composition.
28962762	4	12	theme	inclusion	934:942	arg1	geometries					944:953	the proposed inclusion geometries	921:953	the proposed inclusion geometries	921:953	A theoretical study using DFT and molecular modelling was conducted to obtain the more stable tautomeric species of TG and MP in solution and confirm the proposed inclusion geometries.
28962762	4	13	theme	proposed	925:932	arg1	geometries					944:953	the proposed inclusion geometries	921:953	the proposed inclusion geometries	921:953	A theoretical study using DFT and molecular modelling was conducted to obtain the more stable tautomeric species of TG and MP in solution and confirm the proposed inclusion geometries.
28962762	3	14	theme	powder	685:690	arg1	diffraction					698:708	powder X-ray diffraction	685:708	powder X-ray diffraction	685:708	The formation of the complexes was confirmed using powder X-ray diffraction, thermogravimetric analysis and one and two-dimensional NMR.
28962762	1	15	theme	ternary	414:420	arg1	systems					422:428	the ternary systems	410:428	the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs	410:459	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	15	theme	ternary	414:420	arg1	βCD-MP-AuNPs					448:459	βCD-MP-AuNPs	448:459	βCD-MP-AuNPs	448:459	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	15	theme	ternary	414:420	arg1	βCD-TG-AuNPs					431:442	βCD-TG-AuNPs	431:442	βCD-TG-AuNPs	431:442	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	7	16	dep	in	1245:1246	arg1	vitro					1248:1252	vitro	1248:1252	vitro	1248:1252	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
28962762	1	17	theme	6-thioguanine	181:193	arg1	disadvantages					164:176	the therapeutic disadvantages	148:176	the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP)	148:224	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	3	18	theme	X-ray	692:696	arg1	diffraction					698:708	powder X-ray diffraction	685:708	powder X-ray diffraction	685:708	The formation of the complexes was confirmed using powder X-ray diffraction, thermogravimetric analysis and one and two-dimensional NMR.
28962762	2	19	theme	nanometric	587:596	arg1	dimensions					598:607	their nanometric dimensions	581:607	their nanometric dimensions	581:607	This modification increased their solubility and improved their stability, betting by a site-specific transport due to their nanometric dimensions, among other advantages.
28962762	7	20	theme	ternary	1274:1280	arg1	systems					1282:1288	the ternary systems	1270:1288	the ternary systems	1270:1288	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
28962762	1	21	dep	complexes	302:310	arg1	complexes					302:310	the complexes βCD-TG and βCD-MP	298:328	the complexes βCD-TG and βCD-MP	298:328	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	21	dep	complexes	302:310	arg1	βCD-MP					323:328	βCD-MP	323:328	βCD-MP	323:328	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	21	dep	complexes	302:310	arg1	βCD-TG					312:317	βCD-TG	312:317	βCD-TG	312:317	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	22	with	interaction	354:364	arg1	nanoparticles					376:388	gold nanoparticles	371:388	gold nanoparticles (AuNPs)	371:396	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	22	with	interaction	354:364	arg1	AuNPs					391:395	AuNPs	391:395	AuNPs	391:395	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	7	23	theme	systems	1282:1288	arg1	permeability					1254:1265	the in vitro permeability	1241:1265	the in vitro permeability of the ternary systems	1241:1288	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
28962762	1	24	theme	drugs	261:265	arg1	strategy					119:126	a novel strategy	111:126	a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP)	111:224	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	24	theme	drugs	261:265	arg1	inclusion					242:250	the inclusion	238:250	the inclusion of these drugs in βcyclodextrin (βCD)	238:288	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	25	from	inclusion	242:250	arg1	βCD					285:287	βCD	285:287	βCD	285:287	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	25	from	inclusion	242:250	arg1	βcyclodextrin					270:282	βcyclodextrin	270:282	βcyclodextrin (βCD)	270:288	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	0	26	theme	6-thioguanine	16:28	arg1	Construction					0:11	Construction	0:11	Construction of 6-thioguanine and 6-mercaptopurine carriers	0:58	Construction of 6-thioguanine and 6-mercaptopurine carriers based on βcyclodextrins and gold nanoparticles.
28962762	3	27	theme	thermogravimetric	711:727	arg1	analysis					729:736	thermogravimetric analysis	711:736	thermogravimetric analysis	711:736	The formation of the complexes was confirmed using powder X-ray diffraction, thermogravimetric analysis and one and two-dimensional NMR.
28962762	4	28	theme	MP	894:895	arg1	species					876:882	the more stable tautomeric species	849:882	the more stable tautomeric species of TG and MP	849:895	A theoretical study using DFT and molecular modelling was conducted to obtain the more stable tautomeric species of TG and MP in solution and confirm the proposed inclusion geometries.
28962762	1	29	theme	novel	113:117	arg1	strategy					119:126	a novel strategy	111:126	a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP)	111:224	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	29	theme	novel	113:117	arg1	inclusion					242:250	the inclusion	238:250	the inclusion of these drugs in βcyclodextrin (βCD)	238:288	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	7	30	theme	membrane	1328:1335	arg1	assay					1350:1354	parallel artificial membrane permeability assay	1308:1354	parallel artificial membrane permeability assay (PAMPA)	1308:1362	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
28962762	7	30	theme	membrane	1328:1335	arg1	PAMPA					1357:1361	PAMPA	1357:1361	PAMPA	1357:1361	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
28962762	1	31	theme	subsequent	343:352	arg1	interaction					354:364	subsequent interaction	343:364	subsequent interaction with gold nanoparticles (AuNPs)	343:396	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	32	theme	6-mercaptopurine	204:219	arg1	disadvantages					164:176	the therapeutic disadvantages	148:176	the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP)	148:224	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	0	33	theme	6-mercaptopurine	34:49	arg1	Construction					0:11	Construction	0:11	Construction of 6-thioguanine and 6-mercaptopurine carriers	0:58	Construction of 6-thioguanine and 6-mercaptopurine carriers based on βcyclodextrins and gold nanoparticles.
28962762	6	34	theme	size	1198:1201	arg1	dispersion					1203:1212	size dispersion	1198:1212	size dispersion	1198:1212	Subsequently, the ternary systems were characterized by TEM, FE-SEM and EDX to directly observe the deposited AuNPs and evaluate their sizes, size dispersion, and composition.
28962762	7	35	theme	permeability	1337:1348	arg1	assay					1350:1354	parallel artificial membrane permeability assay	1308:1354	parallel artificial membrane permeability assay (PAMPA)	1308:1362	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
28962762	7	35	theme	permeability	1337:1348	arg1	PAMPA					1357:1361	PAMPA	1357:1361	PAMPA	1357:1361	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
28962762	1	36	theme	gold	371:374	arg1	nanoparticles					376:388	gold nanoparticles	371:388	gold nanoparticles (AuNPs)	371:396	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	1	36	theme	gold	371:374	arg1	AuNPs					391:395	AuNPs	391:395	AuNPs	391:395	As a novel strategy to overcome some of the therapeutic disadvantages of 6-thioguanine (TG) and 6-mercaptopurine (MP), we propose the inclusion of these drugs in βcyclodextrin (βCD) to form the complexes βCD-TG and βCD-MP, followed by subsequent interaction with gold nanoparticles (AuNPs), generating the ternary systems: βCD-TG-AuNPs and βCD-MP-AuNPs.
28962762	4	37	theme	molecular	805:813	arg1	modelling					815:823	molecular modelling	805:823	molecular modelling	805:823	A theoretical study using DFT and molecular modelling was conducted to obtain the more stable tautomeric species of TG and MP in solution and confirm the proposed inclusion geometries.
28962762	7	38	theme	in	1245:1246	arg1	permeability					1254:1265	the in vitro permeability	1241:1265	the in vitro permeability of the ternary systems	1241:1288	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
28962762	3	39	theme	two-dimensional	750:764	arg1	NMR					766:768	two-dimensional NMR	750:768	two-dimensional NMR	750:768	The formation of the complexes was confirmed using powder X-ray diffraction, thermogravimetric analysis and one and two-dimensional NMR.
28962762	7	40	theme	artificial	1317:1326	arg1	assay					1350:1354	parallel artificial membrane permeability assay	1308:1354	parallel artificial membrane permeability assay (PAMPA)	1308:1362	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
28962762	7	40	theme	artificial	1317:1326	arg1	PAMPA					1357:1361	PAMPA	1357:1361	PAMPA	1357:1361	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
28962762	4	41	theme	theoretical	773:783	arg1	study					785:789	A theoretical study	771:789	A theoretical study using DFT and molecular modelling	771:823	A theoretical study using DFT and molecular modelling was conducted to obtain the more stable tautomeric species of TG and MP in solution and confirm the proposed inclusion geometries.
28962762	4	42	theme	TG	887:888	arg1	species					876:882	the more stable tautomeric species	849:882	the more stable tautomeric species of TG and MP	849:895	A theoretical study using DFT and molecular modelling was conducted to obtain the more stable tautomeric species of TG and MP in solution and confirm the proposed inclusion geometries.
28962762	0	43	dep	6-thioguanine	16:28	arg1	carriers					51:58	carriers	51:58	carriers	51:58	Construction of 6-thioguanine and 6-mercaptopurine carriers based on βcyclodextrins and gold nanoparticles.
28962762	5	44	theme	UV-vis	1035:1040	arg1	spectroscopy					1042:1053	UV-vis spectroscopy	1035:1053	UV-vis spectroscopy	1035:1053	The deposition of AuNPs onto βCD-TG and βCD-MP via sputtering was confirmed by UV-vis spectroscopy.
28962762	7	45	theme	parallel	1308:1315	arg1	assay					1350:1354	parallel artificial membrane permeability assay	1308:1354	parallel artificial membrane permeability assay (PAMPA)	1308:1362	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
28962762	7	45	theme	parallel	1308:1315	arg1	PAMPA					1357:1361	PAMPA	1357:1361	PAMPA	1357:1361	Finally, the in vitro permeability of the ternary systems was studied using parallel artificial membrane permeability assay (PAMPA).
26102296	3	0	theme	water	316:320	arg1	extract					322:328	a water extract	314:328	a water extract of Ganoderma lucidum mycelium (WEGL)	314:365	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	3	1	from	inflammation	388:399	arg1	mice					427:430	mice	427:430	mice fed a high-fat diet (HFD)	427:456	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	5	2	theme	WEGL-treated	828:839	arg1	mice					841:844	WEGL-treated mice	828:844	WEGL-treated mice	828:844	The anti-obesity and microbiota-modulating effects are transmissible via horizontal faeces transfer from WEGL-treated mice to HFD-fed mice.
26102296	5	3	theme	faeces	807:812	arg1	transfer					814:821	horizontal faeces transfer	796:821	horizontal faeces transfer from WEGL-treated mice to HFD-fed mice	796:860	The anti-obesity and microbiota-modulating effects are transmissible via horizontal faeces transfer from WEGL-treated mice to HFD-fed mice.
26102296	4	4	theme	decreased	551:559	arg1	ratios					589:594	the decreased Firmicutes-to-Bacteroidetes ratios	547:594	the decreased Firmicutes-to-Bacteroidetes ratios	547:594	Our data indicate that WEGL not only reverses HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels-but also maintains intestinal barrier integrity and reduces metabolic endotoxemia.
26102296	6	5	theme	anti-obesity	980:991	arg1	effects					1019:1025	similar anti-obesity and microbiota-modulating effects	972:1025	similar anti-obesity and microbiota-modulating effects	972:1025	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	6	6	theme	similar	972:978	arg1	effects					1019:1025	similar anti-obesity and microbiota-modulating effects	972:1025	similar anti-obesity and microbiota-modulating effects	972:1025	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	1	7	theme	intestinal	157:166	arg1	dysbiosis					168:176	intestinal dysbiosis	157:176	intestinal dysbiosis	157:176	Obesity is associated with low-grade chronic inflammation and intestinal dysbiosis.
26102296	7	8	theme	obese	1211:1215	arg1	individuals					1217:1227	obese individuals	1211:1227	obese individuals	1211:1227	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	0	9	theme	microbiota	83:92	arg1	composition					60:70	the composition	56:70	the composition of the gut microbiota	56:92	Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota.
26102296	7	10	theme	G.	1054:1055	arg1	polysaccharides					1095:1109	G. lucidum and its high molecular weight polysaccharides	1054:1109	G. lucidum and its high molecular weight polysaccharides	1054:1109	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	7	10	theme	G.	1054:1055	arg1	agents					1136:1141	prebiotic agents	1126:1141	prebiotic agents	1126:1141	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	0	11	theme	gut	79:81	arg1	microbiota					83:92	the gut microbiota	75:92	the gut microbiota	75:92	Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota.
26102296	4	12	theme	Firmicutes-to-Bacteroidetes	561:587	arg1	ratios					589:594	the decreased Firmicutes-to-Bacteroidetes ratios	547:594	the decreased Firmicutes-to-Bacteroidetes ratios	547:594	Our data indicate that WEGL not only reverses HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels-but also maintains intestinal barrier integrity and reduces metabolic endotoxemia.
26102296	3	13	theme	lucidum	343:349	arg1	mycelium					351:358	Ganoderma lucidum mycelium	333:358	Ganoderma lucidum mycelium (WEGL)	333:365	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	3	13	theme	lucidum	343:349	arg1	WEGL					361:364	WEGL	361:364	WEGL	361:364	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	6	14	theme	WEGL	951:954	arg1	extract					956:962	the WEGL extract	947:962	the WEGL extract	947:962	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	7	15	theme	obesity-related	1172:1186	arg1	disorders					1198:1206	obesity-related metabolic disorders	1172:1206	obesity-related metabolic disorders	1172:1206	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	3	16	theme	mycelium	351:358	arg1	extract					322:328	a water extract	314:328	a water extract of Ganoderma lucidum mycelium (WEGL)	314:365	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	7	17	theme	metabolic	1188:1196	arg1	disorders					1198:1206	obesity-related metabolic disorders	1172:1206	obesity-related metabolic disorders	1172:1206	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	7	18	theme	gut	1154:1156	arg1	dysbiosis					1158:1166	gut dysbiosis	1154:1166	gut dysbiosis	1154:1166	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	5	19	theme	horizontal	796:805	arg1	transfer					814:821	horizontal faeces transfer	796:821	horizontal faeces transfer from WEGL-treated mice to HFD-fed mice	796:860	The anti-obesity and microbiota-modulating effects are transmissible via horizontal faeces transfer from WEGL-treated mice to HFD-fed mice.
26102296	3	20	theme	body	375:378	arg1	weight					380:385	body weight	375:385	body weight	375:385	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	3	21	from	weight	380:385	arg1	mice					427:430	mice	427:430	mice fed a high-fat diet (HFD)	427:456	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	4	22	theme	metabolic	700:708	arg1	endotoxemia					710:720	metabolic endotoxemia	700:720	metabolic endotoxemia	700:720	Our data indicate that WEGL not only reverses HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels-but also maintains intestinal barrier integrity and reduces metabolic endotoxemia.
26102296	0	23	theme	Ganoderma	0:8	arg1	lucidum					10:16	Ganoderma lucidum	0:16	Ganoderma lucidum	0:16	Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota.
26102296	7	24	from	dysbiosis	1158:1166	arg1	individuals					1217:1227	obese individuals	1211:1227	obese individuals	1211:1227	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	6	25	theme	high	884:887	arg1	>300 kDa					923:930	>300 kDa	923:930	>300 kDa	923:930	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	6	25	theme	high	884:887	arg1	polysaccharides					906:920	high molecular weight polysaccharides	884:920	high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract	884:962	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	4	26	theme	gut	517:519	arg1	dysbiosis-as					521:532	HFD-induced gut dysbiosis-as	505:532	HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios	505:594	Our data indicate that WEGL not only reverses HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels-but also maintains intestinal barrier integrity and reduces metabolic endotoxemia.
26102296	5	27	theme	microbiota-modulating	744:764	arg1	transmissible					778:790	transmissible	778:790	transmissible	778:790	The anti-obesity and microbiota-modulating effects are transmissible via horizontal faeces transfer from WEGL-treated mice to HFD-fed mice.
26102296	5	27	theme	microbiota-modulating	744:764	arg1	effects					766:772	The anti-obesity and microbiota-modulating effects	723:772	The anti-obesity and microbiota-modulating effects	723:772	The anti-obesity and microbiota-modulating effects are transmissible via horizontal faeces transfer from WEGL-treated mice to HFD-fed mice.
26102296	7	28	used	used	1118:1121	arg2	polysaccharides					1095:1109	G. lucidum and its high molecular weight polysaccharides	1054:1109	G. lucidum and its high molecular weight polysaccharides	1054:1109	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	7	28	used	used	1118:1121	arg2	agents					1136:1141	prebiotic agents	1126:1141	prebiotic agents	1126:1141	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	2	29	theme	Ganoderma	179:187	arg1	lucidum					189:195	Ganoderma lucidum	179:195	Ganoderma lucidum	179:195	Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects.
26102296	2	29	theme	Ganoderma	179:187	arg1	mushroom					212:219	a medicinal mushroom	200:219	a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects	200:292	Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects.
26102296	2	30	theme	Chinese	241:247	arg1	medicine					249:256	traditional Chinese medicine	229:256	traditional Chinese medicine with putative anti-diabetic effects	229:292	Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects.
26102296	4	31	theme	barrier	670:676	arg1	integrity					678:686	intestinal barrier integrity	659:686	intestinal barrier integrity	659:686	Our data indicate that WEGL not only reverses HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels-but also maintains intestinal barrier integrity and reduces metabolic endotoxemia.
26102296	3	32	theme	insulin	405:411	arg1	resistance					413:422	insulin resistance	405:422	insulin resistance	405:422	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	4	33	dep	WEGL	482:485	arg1	only					491:494	only	491:494	only	491:494	Our data indicate that WEGL not only reverses HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels-but also maintains intestinal barrier integrity and reduces metabolic endotoxemia.
26102296	5	34	theme	HFD-fed	849:855	arg1	mice					857:860	HFD-fed mice	849:860	HFD-fed mice	849:860	The anti-obesity and microbiota-modulating effects are transmissible via horizontal faeces transfer from WEGL-treated mice to HFD-fed mice.
26102296	2	35	theme	traditional	229:239	arg1	medicine					249:256	traditional Chinese medicine	229:256	traditional Chinese medicine with putative anti-diabetic effects	229:292	Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects.
26102296	3	36	theme	high-fat	438:445	arg1	HFD					453:455	HFD	453:455	HFD	453:455	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	3	36	theme	high-fat	438:445	arg1	diet					447:450	a high-fat diet	436:450	a high-fat diet (HFD)	436:456	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	2	37	with	medicine	249:256	arg1	effects					286:292	putative anti-diabetic effects	263:292	putative anti-diabetic effects	263:292	Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects.
26102296	7	38	from	disorders	1198:1206	arg1	individuals					1217:1227	obese individuals	1211:1227	obese individuals	1211:1227	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	6	39	theme	weight	899:904	arg1	>300 kDa					923:930	>300 kDa	923:930	>300 kDa	923:930	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	6	39	theme	weight	899:904	arg1	polysaccharides					906:920	high molecular weight polysaccharides	884:920	high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract	884:962	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	4	40	theme	HFD-induced	505:515	arg1	dysbiosis-as					521:532	HFD-induced gut dysbiosis-as	505:532	HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios	505:594	Our data indicate that WEGL not only reverses HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels-but also maintains intestinal barrier integrity and reduces metabolic endotoxemia.
26102296	7	41	theme	prebiotic	1126:1134	arg1	polysaccharides					1095:1109	G. lucidum and its high molecular weight polysaccharides	1054:1109	G. lucidum and its high molecular weight polysaccharides	1054:1109	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	7	41	theme	prebiotic	1126:1134	arg1	agents					1136:1141	prebiotic agents	1126:1141	prebiotic agents	1126:1141	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	5	42	from	mice	841:844	arg1	transfer					814:821	horizontal faeces transfer	796:821	horizontal faeces transfer from WEGL-treated mice to HFD-fed mice	796:860	The anti-obesity and microbiota-modulating effects are transmissible via horizontal faeces transfer from WEGL-treated mice to HFD-fed mice.
26102296	7	43	theme	molecular	1078:1086	arg1	weight					1088:1093	its high molecular weight	1069:1093	its high molecular weight	1069:1093	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	6	44	theme	microbiota-modulating	997:1017	arg1	effects					1019:1025	similar anti-obesity and microbiota-modulating effects	972:1025	similar anti-obesity and microbiota-modulating effects	972:1025	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	2	45	theme	medicinal	202:210	arg1	lucidum					189:195	Ganoderma lucidum	179:195	Ganoderma lucidum	179:195	Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects.
26102296	2	45	theme	medicinal	202:210	arg1	mushroom					212:219	a medicinal mushroom	200:219	a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects	200:292	Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects.
26102296	2	46	theme	anti-diabetic	272:284	arg1	effects					286:292	putative anti-diabetic effects	263:292	putative anti-diabetic effects	263:292	Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects.
26102296	7	47	theme	weight	1088:1093	arg1	polysaccharides					1095:1109	G. lucidum and its high molecular weight polysaccharides	1054:1109	G. lucidum and its high molecular weight polysaccharides	1054:1109	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	7	47	theme	weight	1088:1093	arg1	agents					1136:1141	prebiotic agents	1126:1141	prebiotic agents	1126:1141	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	4	48	theme	endotoxin-bearing	600:616	arg1	levels-but					633:642	endotoxin-bearing Proteobacteria levels-but	600:642	endotoxin-bearing Proteobacteria levels-but	600:642	Our data indicate that WEGL not only reverses HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels-but also maintains intestinal barrier integrity and reduces metabolic endotoxemia.
26102296	5	49	theme	anti-obesity	727:738	arg1	transmissible					778:790	transmissible	778:790	transmissible	778:790	The anti-obesity and microbiota-modulating effects are transmissible via horizontal faeces transfer from WEGL-treated mice to HFD-fed mice.
26102296	5	49	theme	anti-obesity	727:738	arg1	effects					766:772	The anti-obesity and microbiota-modulating effects	723:772	The anti-obesity and microbiota-modulating effects	723:772	The anti-obesity and microbiota-modulating effects are transmissible via horizontal faeces transfer from WEGL-treated mice to HFD-fed mice.
26102296	6	50	theme	molecular	889:897	arg1	>300 kDa					923:930	>300 kDa	923:930	>300 kDa	923:930	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	6	50	theme	molecular	889:897	arg1	polysaccharides					906:920	high molecular weight polysaccharides	884:920	high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract	884:962	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	2	51	theme	putative	263:270	arg1	effects					286:292	putative anti-diabetic effects	263:292	putative anti-diabetic effects	263:292	Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects.
26102296	3	52	from	resistance	413:422	arg1	mice					427:430	mice	427:430	mice fed a high-fat diet (HFD)	427:456	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	1	53	theme	low-grade	122:130	arg1	inflammation					140:151	low-grade chronic inflammation	122:151	low-grade chronic inflammation	122:151	Obesity is associated with low-grade chronic inflammation and intestinal dysbiosis.
26102296	4	54	theme	intestinal	659:668	arg1	integrity					678:686	intestinal barrier integrity	659:686	intestinal barrier integrity	659:686	Our data indicate that WEGL not only reverses HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels-but also maintains intestinal barrier integrity and reduces metabolic endotoxemia.
26102296	7	55	theme	high	1073:1076	arg1	weight					1088:1093	its high molecular weight	1069:1093	its high molecular weight	1069:1093	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	1	56	theme	chronic	132:138	arg1	inflammation					140:151	low-grade chronic inflammation	122:151	low-grade chronic inflammation	122:151	Obesity is associated with low-grade chronic inflammation and intestinal dysbiosis.
26102296	3	57	theme	Ganoderma	333:341	arg1	lucidum					343:349	Ganoderma lucidum	333:349	Ganoderma lucidum mycelium (WEGL)	333:365	Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD).
26102296	0	58	from	obesity	26:32	arg1	mice					37:40	mice	37:40	mice	37:40	Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota.
26102296	7	59	theme	lucidum	1057:1063	arg1	polysaccharides					1095:1109	G. lucidum and its high molecular weight polysaccharides	1054:1109	G. lucidum and its high molecular weight polysaccharides	1054:1109	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	7	59	theme	lucidum	1057:1063	arg1	agents					1136:1141	prebiotic agents	1126:1141	prebiotic agents	1126:1141	Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.
26102296	6	60	attach	isolated	933:940	arg2	>300 kDa					923:930	>300 kDa	923:930	>300 kDa	923:930	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	6	60	attach	isolated	933:940	arg1	extract					956:962	the WEGL extract	947:962	the WEGL extract	947:962	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	6	60	attach	isolated	933:940	arg2	polysaccharides					906:920	high molecular weight polysaccharides	884:920	high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract	884:962	We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects.
26102296	4	61	theme	Proteobacteria	618:631	arg1	levels-but					633:642	endotoxin-bearing Proteobacteria levels-but	600:642	endotoxin-bearing Proteobacteria levels-but	600:642	Our data indicate that WEGL not only reverses HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels-but also maintains intestinal barrier integrity and reduces metabolic endotoxemia.
25772416	0	0	theme	particulate	69:79	arg1	systems					90:96	topical particulate delivery systems	61:96	topical particulate delivery systems	61:96	Development and validation of an in vitro release method for topical particulate delivery systems.
25772416	9	1	dep	in	1258:1259	arg1	vitro					1261:1265	vitro	1261:1265	vitro	1261:1265	Currently there are no compendial or standard methods and apparatuses for in vitro release testing of topical microparticles.
25772416	7	2	theme	medium	1048:1053	arg1	cm					1022:1023	1cm, 2.5 cm	1013:1023	1cm, 2.5 cm	1013:1023	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	2	theme	medium	1048:1053	arg1	volume					1030:1035	volume	1030:1035	volume of release medium (100ml, 200 ml)	1030:1069	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	2	theme	medium	1048:1053	arg1	speed					965:969	paddle rotation speed	949:969	paddle rotation speed (25 rpm, 50 rpm, 100 rpm)	949:995	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	2	theme	medium	1048:1053	arg1	100ml					1056:1060	100ml	1056:1060	100ml	1056:1060	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	2	theme	medium	1048:1053	arg1	height					1005:1010	paddle height	998:1010	paddle height (1cm, 2.5 cm)	998:1024	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	9	3	theme	microparticles	1294:1307	arg1	testing					1275:1281	in vitro release testing	1258:1281	in vitro release testing of topical microparticles	1258:1307	Currently there are no compendial or standard methods and apparatuses for in vitro release testing of topical microparticles.
25772416	9	4	theme	compendial	1207:1216	arg1	methods					1230:1236	methods	1230:1236	methods	1230:1236	Currently there are no compendial or standard methods and apparatuses for in vitro release testing of topical microparticles.
25772416	0	5	theme	topical	61:67	arg1	systems					90:96	topical particulate delivery systems	61:96	topical particulate delivery systems	61:96	Development and validation of an in vitro release method for topical particulate delivery systems.
25772416	3	6	theme	low	514:516	arg1	adsorption					523:532	low drug adsorption	514:532	low drug adsorption	514:532	Diffusion of the drug occurred across a mixed cellulose ester membrane, which demonstrated low drug adsorption and low diffusional resistance.
25772416	4	7	theme	lag	583:585	arg1	phase					587:591	an initial lag phase	572:591	an initial lag phase	572:591	After an initial lag phase the amount of drug released became proportional to the square root of time.
25772416	7	8	theme	release	1103:1109	arg1	rates					1111:1115	the release rates	1099:1115	the release rates	1099:1115	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	10	9	theme	useful	1340:1345	arg1	guide					1347:1351	a useful guide	1338:1351	a useful guide in formulation development of such delivery systems	1338:1403	The developed method can be a useful guide in formulation development of such delivery systems.
25772416	10	9	theme	useful	1340:1345	arg1	method					1324:1329	The developed method	1310:1329	The developed method	1310:1329	The developed method can be a useful guide in formulation development of such delivery systems.
25772416	2	10	used	used	360:363	arg2	microparticles					312:325	Chitosan- and methacrylate-based microparticles	279:325	Chitosan- and methacrylate-based microparticles with mupirocin	279:340	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	2	10	used	used	360:363	arg2	systems					391:397	model topical delivery systems	368:397	model topical delivery systems for method development	368:420	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	3	11	theme	diffusional	542:552	arg1	resistance					554:563	low diffusional resistance	538:563	low diffusional resistance	538:563	Diffusion of the drug occurred across a mixed cellulose ester membrane, which demonstrated low drug adsorption and low diffusional resistance.
25772416	2	12	with	microparticles	312:325	arg1	mupirocin					332:340	mupirocin	332:340	mupirocin	332:340	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	0	13	theme	delivery	81:88	arg1	systems					90:96	topical particulate delivery systems	61:96	topical particulate delivery systems	61:96	Development and validation of an in vitro release method for topical particulate delivery systems.
25772416	6	14	theme	composition	893:903	arg1	batches					873:879	batches	873:879	batches of the same composition prepared by the same process	873:932	The method was further used to confirm sameness between batches of the same composition prepared by the same process.
25772416	7	15	theme	release	1040:1046	arg1	medium					1048:1053	release medium	1040:1053	release medium	1040:1053	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	16	theme	paddle	949:954	arg1	speed					965:969	paddle rotation speed	949:969	paddle rotation speed (25 rpm, 50 rpm, 100 rpm)	949:995	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	5	17	from	differences	706:716	arg1	formulation					721:731	formulation	721:731	formulation	721:731	The method was discriminative toward differences in formulation, as well as toward differences in drug concentration inside the sample compartment.
25772416	6	18	theme	same	888:891	arg1	composition					893:903	the same composition	884:903	the same composition prepared by the same process	884:932	The method was further used to confirm sameness between batches of the same composition prepared by the same process.
25772416	7	19	dep	100ml	1056:1060	arg1	ml					1067:1068	200 ml	1063:1068	200 ml	1063:1068	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	3	20	theme	cellulose	469:477	arg1	membrane					485:492	a mixed cellulose ester membrane	461:492	a mixed cellulose ester membrane	461:492	Diffusion of the drug occurred across a mixed cellulose ester membrane, which demonstrated low drug adsorption and low diffusional resistance.
25772416	1	21	theme	release	148:154	arg1	method					156:161	an in vitro release method	136:161	an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus	136:276	The aim of this study was to develop an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus.
25772416	1	22	theme	in	139:140	arg1	method					156:161	an in vitro release method	136:161	an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus	136:276	The aim of this study was to develop an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus.
25772416	9	23	theme	standard	1221:1228	arg1	methods					1230:1236	methods	1230:1236	methods	1230:1236	Currently there are no compendial or standard methods and apparatuses for in vitro release testing of topical microparticles.
25772416	3	24	theme	low	538:540	arg1	resistance					554:563	low diffusional resistance	538:563	low diffusional resistance	538:563	Diffusion of the drug occurred across a mixed cellulose ester membrane, which demonstrated low drug adsorption and low diffusional resistance.
25772416	8	25	theme	analysis	1132:1139	arg1	method					1122:1127	The method	1118:1127	The method of analysis	1118:1139	The method of analysis was validated according to ICH guidelines.
25772416	5	26	theme	drug	767:770	arg1	concentration					772:784	drug concentration	767:784	drug concentration inside the sample compartment	767:814	The method was discriminative toward differences in formulation, as well as toward differences in drug concentration inside the sample compartment.
25772416	3	27	theme	drug	518:521	arg1	adsorption					523:532	low drug adsorption	514:532	low drug adsorption	514:532	Diffusion of the drug occurred across a mixed cellulose ester membrane, which demonstrated low drug adsorption and low diffusional resistance.
25772416	2	28	theme	delivery	382:389	arg1	systems					391:397	model topical delivery systems	368:397	model topical delivery systems for method development	368:420	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	2	28	theme	delivery	382:389	arg1	microparticles					312:325	Chitosan- and methacrylate-based microparticles	279:325	Chitosan- and methacrylate-based microparticles with mupirocin	279:340	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	1	29	theme	paddle	249:254	arg1	apparatus					268:276	paddle dissolution apparatus	249:276	paddle dissolution apparatus	249:276	The aim of this study was to develop an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus.
25772416	5	30	from	differences	752:762	arg1	concentration					772:784	drug concentration	767:784	drug concentration inside the sample compartment	767:814	The method was discriminative toward differences in formulation, as well as toward differences in drug concentration inside the sample compartment.
25772416	2	31	theme	methacrylate-based	293:310	arg1	systems					391:397	model topical delivery systems	368:397	model topical delivery systems for method development	368:420	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	2	31	theme	methacrylate-based	293:310	arg1	microparticles					312:325	Chitosan- and methacrylate-based microparticles	279:325	Chitosan- and methacrylate-based microparticles with mupirocin	279:340	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	2	32	theme	topical	374:380	arg1	systems					391:397	model topical delivery systems	368:397	model topical delivery systems for method development	368:420	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	2	32	theme	topical	374:380	arg1	microparticles					312:325	Chitosan- and methacrylate-based microparticles	279:325	Chitosan- and methacrylate-based microparticles with mupirocin	279:340	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	6	33	used	used	840:843	arg2	method					821:826	The method	817:826	The method	817:826	The method was further used to confirm sameness between batches of the same composition prepared by the same process.
25772416	1	34	theme	dissolution	256:266	arg1	apparatus					268:276	paddle dissolution apparatus	249:276	paddle dissolution apparatus	249:276	The aim of this study was to develop an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus.
25772416	7	35	theme	rotation	956:963	arg1	speed					965:969	paddle rotation speed	949:969	paddle rotation speed (25 rpm, 50 rpm, 100 rpm)	949:995	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	10	36	theme	formulation	1356:1366	arg1	development					1368:1378	formulation development	1356:1378	formulation development of such delivery systems	1356:1403	The developed method can be a useful guide in formulation development of such delivery systems.
25772416	3	37	theme	mixed	463:467	arg1	membrane					485:492	a mixed cellulose ester membrane	461:492	a mixed cellulose ester membrane	461:492	Diffusion of the drug occurred across a mixed cellulose ester membrane, which demonstrated low drug adsorption and low diffusional resistance.
25772416	4	38	theme	initial	575:581	arg1	phase					587:591	an initial lag phase	572:591	an initial lag phase	572:591	After an initial lag phase the amount of drug released became proportional to the square root of time.
25772416	2	39	theme	Chitosan-	279:287	arg1	systems					391:397	model topical delivery systems	368:397	model topical delivery systems for method development	368:420	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	2	39	theme	Chitosan-	279:287	arg1	microparticles					312:325	Chitosan- and methacrylate-based microparticles	279:325	Chitosan- and methacrylate-based microparticles with mupirocin	279:340	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	1	40	theme	topical	167:173	arg1	systems					196:202	topical particulate delivery systems	167:202	topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus	167:276	The aim of this study was to develop an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus.
25772416	0	41	theme	in	33:34	arg1	method					50:55	an in vitro release method	30:55	an in vitro release method for topical particulate delivery systems	30:96	Development and validation of an in vitro release method for topical particulate delivery systems.
25772416	9	42	theme	in	1258:1259	arg1	testing					1275:1281	in vitro release testing	1258:1281	in vitro release testing of topical microparticles	1258:1307	Currently there are no compendial or standard methods and apparatuses for in vitro release testing of topical microparticles.
25772416	10	43	theme	systems	1397:1403	arg1	development					1368:1378	formulation development	1356:1378	formulation development of such delivery systems	1356:1403	The developed method can be a useful guide in formulation development of such delivery systems.
25772416	4	44	theme	square	648:653	arg1	root					655:658	the square root	644:658	the square root of time	644:666	After an initial lag phase the amount of drug released became proportional to the square root of time.
25772416	4	45	theme	time	663:666	arg1	root					655:658	the square root	644:658	the square root of time	644:666	After an initial lag phase the amount of drug released became proportional to the square root of time.
25772416	1	46	theme	particulate	175:185	arg1	systems					196:202	topical particulate delivery systems	167:202	topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus	167:276	The aim of this study was to develop an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus.
25772416	3	47	theme	drug	440:443	arg1	Diffusion					423:431	Diffusion	423:431	Diffusion of the drug	423:443	Diffusion of the drug occurred across a mixed cellulose ester membrane, which demonstrated low drug adsorption and low diffusional resistance.
25772416	10	48	theme	delivery	1388:1395	arg1	systems					1397:1403	such delivery systems	1383:1403	such delivery systems	1383:1403	The developed method can be a useful guide in formulation development of such delivery systems.
25772416	1	49	with	combination	232:242	arg1	apparatus					268:276	paddle dissolution apparatus	249:276	paddle dissolution apparatus	249:276	The aim of this study was to develop an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus.
25772416	1	50	theme	delivery	187:194	arg1	systems					196:202	topical particulate delivery systems	167:202	topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus	167:276	The aim of this study was to develop an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus.
25772416	0	51	theme	release	42:48	arg1	method					50:55	an in vitro release method	30:55	an in vitro release method for topical particulate delivery systems	30:96	Development and validation of an in vitro release method for topical particulate delivery systems.
25772416	9	52	theme	release	1267:1273	arg1	testing					1275:1281	in vitro release testing	1258:1281	in vitro release testing of topical microparticles	1258:1307	Currently there are no compendial or standard methods and apparatuses for in vitro release testing of topical microparticles.
25772416	7	53	theme	paddle	998:1003	arg1	cm					1022:1023	1cm, 2.5 cm	1013:1023	1cm, 2.5 cm	1013:1023	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	53	theme	paddle	998:1003	arg1	height					1005:1010	paddle height	998:1010	paddle height (1cm, 2.5 cm)	998:1024	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	1	54	dep	in	139:140	arg1	vitro					142:146	vitro	142:146	vitro	142:146	The aim of this study was to develop an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus.
25772416	10	55	from	guide	1347:1351	arg1	development					1368:1378	formulation development	1356:1378	formulation development of such delivery systems	1356:1403	The developed method can be a useful guide in formulation development of such delivery systems.
25772416	5	56	theme	sample	797:802	arg1	compartment					804:814	the sample compartment	793:814	the sample compartment	793:814	The method was discriminative toward differences in formulation, as well as toward differences in drug concentration inside the sample compartment.
25772416	10	57	theme	developed	1314:1322	arg1	guide					1347:1351	a useful guide	1338:1351	a useful guide in formulation development of such delivery systems	1338:1403	The developed method can be a useful guide in formulation development of such delivery systems.
25772416	10	57	theme	developed	1314:1322	arg1	method					1324:1329	The developed method	1310:1329	The developed method	1310:1329	The developed method can be a useful guide in formulation development of such delivery systems.
25772416	3	58	theme	ester	479:483	arg1	membrane					485:492	a mixed cellulose ester membrane	461:492	a mixed cellulose ester membrane	461:492	Diffusion of the drug occurred across a mixed cellulose ester membrane, which demonstrated low drug adsorption and low diffusional resistance.
25772416	4	59	theme	drug	607:610	arg1	drug					607:610	drug	607:610	drug	607:610	After an initial lag phase the amount of drug released became proportional to the square root of time.
25772416	4	59	theme	drug	607:610	arg1	amount					597:602	the amount	593:602	the amount of drug released	593:619	After an initial lag phase the amount of drug released became proportional to the square root of time.
25772416	0	60	dep	in	33:34	arg1	vitro					36:40	vitro	36:40	vitro	36:40	Development and validation of an in vitro release method for topical particulate delivery systems.
25772416	10	61	theme	such	1383:1386	arg1	systems					1397:1403	such delivery systems	1383:1403	such delivery systems	1383:1403	The developed method can be a useful guide in formulation development of such delivery systems.
25772416	1	62	theme	study	115:119	arg1	aim					103:105	The aim	99:105	The aim of this study	99:119	The aim of this study was to develop an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus.
25772416	7	63	dep	speed	965:969	arg1	rpm					975:977	25 rpm	972:977	25 rpm	972:977	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	63	dep	speed	965:969	arg1	rpm					983:985	50 rpm	980:985	50 rpm	980:985	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	63	dep	speed	965:969	arg1	rpm					992:994	100 rpm	988:994	100 rpm	988:994	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	64	from	Variations	935:944	arg1	cm					1022:1023	1cm, 2.5 cm	1013:1023	1cm, 2.5 cm	1013:1023	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	64	from	Variations	935:944	arg1	height					1005:1010	paddle height	998:1010	paddle height (1cm, 2.5 cm)	998:1024	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	64	from	Variations	935:944	arg1	speed					965:969	paddle rotation speed	949:969	paddle rotation speed (25 rpm, 50 rpm, 100 rpm)	949:995	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	64	from	Variations	935:944	arg1	100ml					1056:1060	100ml	1056:1060	100ml	1056:1060	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	7	64	from	Variations	935:944	arg1	volume					1030:1035	volume	1030:1035	volume of release medium (100ml, 200 ml)	1030:1069	Variations in paddle rotation speed (25 rpm, 50 rpm, 100 rpm), paddle height (1cm, 2.5 cm) and volume of release medium (100ml, 200 ml) did not significantly alter the release rates.
25772416	2	65	theme	method	403:408	arg1	development					410:420	method development	403:420	method development	403:420	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	8	66	theme	ICH	1168:1170	arg1	guidelines					1172:1181	ICH guidelines	1168:1181	ICH guidelines	1168:1181	The method of analysis was validated according to ICH guidelines.
25772416	2	67	theme	model	368:372	arg1	systems					391:397	model topical delivery systems	368:397	model topical delivery systems for method development	368:420	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	2	67	theme	model	368:372	arg1	microparticles					312:325	Chitosan- and methacrylate-based microparticles	279:325	Chitosan- and methacrylate-based microparticles with mupirocin	279:340	Chitosan- and methacrylate-based microparticles with mupirocin were prepared and used as model topical delivery systems for method development.
25772416	0	68	theme	method	50:55	arg1	validation					16:25	validation	16:25	validation	16:25	Development and validation of an in vitro release method for topical particulate delivery systems.
25772416	0	68	theme	method	50:55	arg1	Development					0:10	Development	0:10	Development	0:10	Development and validation of an in vitro release method for topical particulate delivery systems.
25772416	6	69	theme	same	921:924	arg1	process					926:932	the same process	917:932	the same process	917:932	The method was further used to confirm sameness between batches of the same composition prepared by the same process.
25772416	9	70	theme	topical	1286:1292	arg1	microparticles					1294:1307	topical microparticles	1286:1307	topical microparticles	1286:1307	Currently there are no compendial or standard methods and apparatuses for in vitro release testing of topical microparticles.
25772416	1	71	theme	immersion	214:222	arg1	cell					224:227	the immersion cell	210:227	the immersion cell	210:227	The aim of this study was to develop an in vitro release method for topical particulate delivery systems using the immersion cell in combination with paddle dissolution apparatus.
29043796	3	0	theme	sensor	343:348	arg1	design					350:355	The sensor design	339:355	The sensor design	339:355	The sensor design consists of microsized enzymatic sensors that are embedded in an outer hydrogel matrix.
29043796	5	1	theme	lactate	841:847	arg1	challenges					863:872	lactate concentration challenges	841:872	lactate concentration challenges at low physiological oxygen levels	841:907	The response of the composite hydrogel-based lactate sensors was characterized by subjecting the sensors to lactate concentration challenges at low physiological oxygen levels.
29043796	7	2	theme	cyclic	1042:1047	arg1	exposure					1049:1056	Repeated cyclic exposure	1033:1056	Repeated cyclic exposure to high levels of lactate	1033:1082	Repeated cyclic exposure to high levels of lactate revealed that these sensors were extremely stable, with no significant loss in sensor response after 20 cycles.
29043796	6	3	dep	mg/dL	989:993	arg1	mg-1					1013:1016	dL mg-1	1010:1016	9.2 ± 0.83 mg/dL and 11 ± 0.90% dL mg-1	978:1016	The analytical range and the mean sensitivity were determined to be 9.2 ± 0.83 mg/dL and 11 ± 0.90% dL mg-1, respectively.
29043796	5	4	theme	concentration	849:861	arg1	challenges					863:872	lactate concentration challenges	841:872	lactate concentration challenges at low physiological oxygen levels	841:907	The response of the composite hydrogel-based lactate sensors was characterized by subjecting the sensors to lactate concentration challenges at low physiological oxygen levels.
29043796	3	5	from	matrix	437:442	arg1	embedded					407:414	embedded	407:414	embedded	407:414	The sensor design consists of microsized enzymatic sensors that are embedded in an outer hydrogel matrix.
29043796	4	6	theme	lactate	492:498	arg1	permeation					688:697	the permeation	684:697	the permeation of lactate into the microsensors	684:730	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	6	theme	lactate	492:498	arg1	component					532:540	the bioactive component	518:540	the bioactive component	518:540	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	6	theme	lactate	492:498	arg1	oxidase					500:506	encapsulated lactate oxidase	479:506	encapsulated lactate oxidase	479:506	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	7	theme	phosphorescent	543:556	arg1	transducer					595:604	the optical transducer	583:604	the optical transducer	583:604	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	7	theme	phosphorescent	543:556	arg1	metalloporphyrin					558:573	phosphorescent metalloporphyrin	543:573	phosphorescent metalloporphyrin	543:573	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	8	8	contain	have	1320:1323	arg2	potential					1329:1337	the potential	1325:1337	the potential for use as fully implantable optical lactate sensors	1325:1390	These preliminary results support the premise that these composite hydrogels are capable of continuous lactate tracking and have the potential for use as fully implantable optical lactate sensors.
29043796	8	8	contain	have	1320:1323	arg1	hydrogels					1263:1271	these composite hydrogels	1247:1271	these composite hydrogels	1247:1271	These preliminary results support the premise that these composite hydrogels are capable of continuous lactate tracking and have the potential for use as fully implantable optical lactate sensors.
29043796	5	9	theme	low	877:879	arg1	levels					902:907	low physiological oxygen levels	877:907	low physiological oxygen levels	877:907	The response of the composite hydrogel-based lactate sensors was characterized by subjecting the sensors to lactate concentration challenges at low physiological oxygen levels.
29043796	8	10	theme	preliminary	1202:1212	arg1	results					1214:1220	These preliminary results	1196:1220	These preliminary results	1196:1220	These preliminary results support the premise that these composite hydrogels are capable of continuous lactate tracking and have the potential for use as fully implantable optical lactate sensors.
29043796	6	11	theme	±	982:982	arg1	mg/dL					989:993	9.2 ± 0.83 mg/dL and 11 ± 0.90%	978:1008	mg/dL	989:993	The analytical range and the mean sensitivity were determined to be 9.2 ± 0.83 mg/dL and 11 ± 0.90% dL mg-1, respectively.
29043796	6	11	theme	±	982:982	arg1	range					925:929	The analytical range	910:929	The analytical range	910:929	The analytical range and the mean sensitivity were determined to be 9.2 ± 0.83 mg/dL and 11 ± 0.90% dL mg-1, respectively.
29043796	6	11	theme	±	982:982	arg1	sensitivity					944:954	the mean sensitivity	935:954	the mean sensitivity	935:954	The analytical range and the mean sensitivity were determined to be 9.2 ± 0.83 mg/dL and 11 ± 0.90% dL mg-1, respectively.
29043796	7	12	theme	sensor	1163:1168	arg1	response					1170:1177	sensor response	1163:1177	sensor response after 20 cycles	1163:1193	Repeated cyclic exposure to high levels of lactate revealed that these sensors were extremely stable, with no significant loss in sensor response after 20 cycles.
29043796	2	13	theme	optical	309:315	arg1	detection					317:325	optical detection	309:325	optical detection of lactate	309:336	In this work we have demonstrated a composite hydrogel-based sensing platform that may be used for optical detection of lactate.
29043796	3	14	theme	microsized	369:378	arg1	sensors					390:396	microsized enzymatic sensors	369:396	microsized enzymatic sensors that are embedded in an outer hydrogel matrix	369:442	The sensor design consists of microsized enzymatic sensors that are embedded in an outer hydrogel matrix.
29043796	3	15	from	embedded	407:414	arg1	matrix					437:442	an outer hydrogel matrix	419:442	an outer hydrogel matrix	419:442	The sensor design consists of microsized enzymatic sensors that are embedded in an outer hydrogel matrix.
29043796	7	16	theme	significant	1143:1153	arg1	loss					1155:1158	no significant loss	1140:1158	no significant loss in sensor response after 20 cycles	1140:1193	Repeated cyclic exposure to high levels of lactate revealed that these sensors were extremely stable, with no significant loss in sensor response after 20 cycles.
29043796	8	17	theme	lactate	1299:1305	arg1	tracking					1307:1314	continuous lactate tracking	1288:1314	continuous lactate tracking	1288:1314	These preliminary results support the premise that these composite hydrogels are capable of continuous lactate tracking and have the potential for use as fully implantable optical lactate sensors.
29043796	3	18	theme	enzymatic	380:388	arg1	sensors					390:396	microsized enzymatic sensors	369:396	microsized enzymatic sensors that are embedded in an outer hydrogel matrix	369:442	The sensor design consists of microsized enzymatic sensors that are embedded in an outer hydrogel matrix.
29043796	4	19	theme	bioactive	522:530	arg1	permeation					688:697	the permeation	684:697	the permeation of lactate into the microsensors	684:730	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	19	theme	bioactive	522:530	arg1	component					532:540	the bioactive component	518:540	the bioactive component	518:540	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	19	theme	bioactive	522:530	arg1	oxidase					500:506	encapsulated lactate oxidase	479:506	encapsulated lactate oxidase	479:506	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	6	20	theme	mean	939:942	arg1	sensitivity					944:954	the mean sensitivity	935:954	the mean sensitivity	935:954	The analytical range and the mean sensitivity were determined to be 9.2 ± 0.83 mg/dL and 11 ± 0.90% dL mg-1, respectively.
29043796	6	20	theme	mean	939:942	arg1	mg/dL					989:993	9.2 ± 0.83 mg/dL and 11 ± 0.90%	978:1008	mg/dL	989:993	The analytical range and the mean sensitivity were determined to be 9.2 ± 0.83 mg/dL and 11 ± 0.90% dL mg-1, respectively.
29043796	8	21	theme	continuous	1288:1297	arg1	tracking					1307:1314	continuous lactate tracking	1288:1314	continuous lactate tracking	1288:1314	These preliminary results support the premise that these composite hydrogels are capable of continuous lactate tracking and have the potential for use as fully implantable optical lactate sensors.
29043796	5	22	theme	oxygen	895:900	arg1	levels					902:907	low physiological oxygen levels	877:907	low physiological oxygen levels	877:907	The response of the composite hydrogel-based lactate sensors was characterized by subjecting the sensors to lactate concentration challenges at low physiological oxygen levels.
29043796	5	23	from	levels	902:907	arg1	challenges					863:872	lactate concentration challenges	841:872	lactate concentration challenges at low physiological oxygen levels	841:907	The response of the composite hydrogel-based lactate sensors was characterized by subjecting the sensors to lactate concentration challenges at low physiological oxygen levels.
29043796	1	24	theme	monitoring	107:116	arg1	biomarkers					127:136	Continuously monitoring specific biomarkers	94:136	Continuously monitoring specific biomarkers	94:136	Continuously monitoring specific biomarkers offer a promising method to interrogate disease status and progression.
29043796	0	25	theme	Composite	0:8	arg1	Hydrogels					10:18	Composite Hydrogels	0:18	Composite Hydrogels	0:18	Composite Hydrogels Containing Bioactive Microreactors for Optical Enzymatic Lactate Sensing.
29043796	6	26	theme	analytical	914:923	arg1	mg/dL					989:993	9.2 ± 0.83 mg/dL and 11 ± 0.90%	978:1008	mg/dL	989:993	The analytical range and the mean sensitivity were determined to be 9.2 ± 0.83 mg/dL and 11 ± 0.90% dL mg-1, respectively.
29043796	6	26	theme	analytical	914:923	arg1	range					925:929	The analytical range	910:929	The analytical range	910:929	The analytical range and the mean sensitivity were determined to be 9.2 ± 0.83 mg/dL and 11 ± 0.90% dL mg-1, respectively.
29043796	1	27	theme	specific	118:125	arg1	biomarkers					127:136	Continuously monitoring specific biomarkers	94:136	Continuously monitoring specific biomarkers	94:136	Continuously monitoring specific biomarkers offer a promising method to interrogate disease status and progression.
29043796	0	28	theme	Optical	59:65	arg1	Sensing					85:91	Optical Enzymatic Lactate Sensing	59:91	Optical Enzymatic Lactate Sensing	59:91	Composite Hydrogels Containing Bioactive Microreactors for Optical Enzymatic Lactate Sensing.
29043796	8	29	theme	tracking	1307:1314	arg1	capable					1277:1283	capable	1277:1283	capable	1277:1283	These preliminary results support the premise that these composite hydrogels are capable of continuous lactate tracking and have the potential for use as fully implantable optical lactate sensors.
29043796	0	30	theme	Bioactive	31:39	arg1	Microreactors					41:53	Bioactive Microreactors	31:53	Bioactive Microreactors for Optical Enzymatic Lactate Sensing	31:91	Composite Hydrogels Containing Bioactive Microreactors for Optical Enzymatic Lactate Sensing.
29043796	5	31	theme	hydrogel-based	763:776	arg1	sensors					786:792	the composite hydrogel-based lactate sensors	749:792	the composite hydrogel-based lactate sensors	749:792	The response of the composite hydrogel-based lactate sensors was characterized by subjecting the sensors to lactate concentration challenges at low physiological oxygen levels.
29043796	8	32	theme	composite	1253:1261	arg1	hydrogels					1263:1271	these composite hydrogels	1247:1271	these composite hydrogels	1247:1271	These preliminary results support the premise that these composite hydrogels are capable of continuous lactate tracking and have the potential for use as fully implantable optical lactate sensors.
29043796	2	33	theme	lactate	330:336	arg1	detection					317:325	optical detection	309:325	optical detection of lactate	309:336	In this work we have demonstrated a composite hydrogel-based sensing platform that may be used for optical detection of lactate.
29043796	4	34	theme	polyelectrolyte	611:625	arg1	multilayers					627:637	polyelectrolyte multilayers	611:637	polyelectrolyte multilayers coated on the enzymatic microsensors	611:674	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	5	35	theme	lactate	778:784	arg1	sensors					786:792	the composite hydrogel-based lactate sensors	749:792	the composite hydrogel-based lactate sensors	749:792	The response of the composite hydrogel-based lactate sensors was characterized by subjecting the sensors to lactate concentration challenges at low physiological oxygen levels.
29043796	4	36	theme	encapsulated	479:490	arg1	permeation					688:697	the permeation	684:697	the permeation of lactate into the microsensors	684:730	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	36	theme	encapsulated	479:490	arg1	component					532:540	the bioactive component	518:540	the bioactive component	518:540	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	36	theme	encapsulated	479:490	arg1	oxidase					500:506	encapsulated lactate oxidase	479:506	encapsulated lactate oxidase	479:506	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	7	37	from	loss	1155:1158	arg1	response					1170:1177	sensor response	1163:1177	sensor response after 20 cycles	1163:1193	Repeated cyclic exposure to high levels of lactate revealed that these sensors were extremely stable, with no significant loss in sensor response after 20 cycles.
29043796	5	38	theme	sensors	786:792	arg1	response					737:744	The response	733:744	The response of the composite hydrogel-based lactate sensors	733:792	The response of the composite hydrogel-based lactate sensors was characterized by subjecting the sensors to lactate concentration challenges at low physiological oxygen levels.
29043796	2	39	theme	sensing	271:277	arg1	platform					279:286	a composite hydrogel-based sensing platform	244:286	a composite hydrogel-based sensing platform that may be used for optical detection of lactate	244:336	In this work we have demonstrated a composite hydrogel-based sensing platform that may be used for optical detection of lactate.
29043796	7	40	with	stable	1127:1132	arg1	loss					1155:1158	no significant loss	1140:1158	no significant loss in sensor response after 20 cycles	1140:1193	Repeated cyclic exposure to high levels of lactate revealed that these sensors were extremely stable, with no significant loss in sensor response after 20 cycles.
29043796	2	41	theme	hydrogel-based	256:269	arg1	platform					279:286	a composite hydrogel-based sensing platform	244:286	a composite hydrogel-based sensing platform that may be used for optical detection of lactate	244:336	In this work we have demonstrated a composite hydrogel-based sensing platform that may be used for optical detection of lactate.
29043796	4	42	theme	lactate	702:708	arg1	permeation					688:697	the permeation	684:697	the permeation of lactate into the microsensors	684:730	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	42	theme	lactate	702:708	arg1	component					532:540	the bioactive component	518:540	the bioactive component	518:540	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	42	theme	lactate	702:708	arg1	oxidase					500:506	encapsulated lactate oxidase	479:506	encapsulated lactate oxidase	479:506	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	3	43	theme	outer	422:426	arg1	matrix					437:442	an outer hydrogel matrix	419:442	an outer hydrogel matrix	419:442	The sensor design consists of microsized enzymatic sensors that are embedded in an outer hydrogel matrix.
29043796	5	44	theme	physiological	881:893	arg1	levels					902:907	low physiological oxygen levels	877:907	low physiological oxygen levels	877:907	The response of the composite hydrogel-based lactate sensors was characterized by subjecting the sensors to lactate concentration challenges at low physiological oxygen levels.
29043796	0	45	theme	Lactate	77:83	arg1	Sensing					85:91	Optical Enzymatic Lactate Sensing	59:91	Optical Enzymatic Lactate Sensing	59:91	Composite Hydrogels Containing Bioactive Microreactors for Optical Enzymatic Lactate Sensing.
29043796	2	46	theme	composite	246:254	arg1	platform					279:286	a composite hydrogel-based sensing platform	244:286	a composite hydrogel-based sensing platform that may be used for optical detection of lactate	244:336	In this work we have demonstrated a composite hydrogel-based sensing platform that may be used for optical detection of lactate.
29043796	7	47	theme	lactate	1076:1082	arg1	levels					1066:1071	high levels	1061:1071	high levels of lactate	1061:1082	Repeated cyclic exposure to high levels of lactate revealed that these sensors were extremely stable, with no significant loss in sensor response after 20 cycles.
29043796	3	48	theme	hydrogel	428:435	arg1	matrix					437:442	an outer hydrogel matrix	419:442	an outer hydrogel matrix	419:442	The sensor design consists of microsized enzymatic sensors that are embedded in an outer hydrogel matrix.
29043796	4	49	theme	optical	587:593	arg1	transducer					595:604	the optical transducer	583:604	the optical transducer	583:604	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	49	theme	optical	587:593	arg1	metalloporphyrin					558:573	phosphorescent metalloporphyrin	543:573	phosphorescent metalloporphyrin	543:573	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	7	50	theme	high	1061:1064	arg1	levels					1066:1071	high levels	1061:1071	high levels of lactate	1061:1082	Repeated cyclic exposure to high levels of lactate revealed that these sensors were extremely stable, with no significant loss in sensor response after 20 cycles.
29043796	1	51	theme	promising	146:154	arg1	method					156:161	a promising method	144:161	a promising method to interrogate disease status and progression	144:207	Continuously monitoring specific biomarkers offer a promising method to interrogate disease status and progression.
29043796	6	52	theme	±	1002:1002	arg1	%					1008:1008	9.2 ± 0.83 mg/dL and 11 ± 0.90%	978:1008	%	1008:1008	The analytical range and the mean sensitivity were determined to be 9.2 ± 0.83 mg/dL and 11 ± 0.90% dL mg-1, respectively.
29043796	8	53	theme	implantable	1356:1366	arg1	sensors					1384:1390	fully implantable optical lactate sensors	1350:1390	fully implantable optical lactate sensors	1350:1390	These preliminary results support the premise that these composite hydrogels are capable of continuous lactate tracking and have the potential for use as fully implantable optical lactate sensors.
29043796	5	54	theme	composite	753:761	arg1	sensors					786:792	the composite hydrogel-based lactate sensors	749:792	the composite hydrogel-based lactate sensors	749:792	The response of the composite hydrogel-based lactate sensors was characterized by subjecting the sensors to lactate concentration challenges at low physiological oxygen levels.
29043796	8	55	theme	optical	1368:1374	arg1	sensors					1384:1390	fully implantable optical lactate sensors	1350:1390	fully implantable optical lactate sensors	1350:1390	These preliminary results support the premise that these composite hydrogels are capable of continuous lactate tracking and have the potential for use as fully implantable optical lactate sensors.
29043796	4	56	theme	enzymatic	653:661	arg1	microsensors					663:674	the enzymatic microsensors	649:674	the enzymatic microsensors	649:674	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	4	57	theme	engineered	454:463	arg1	microdomains					465:476	these engineered microdomains	448:476	these engineered microdomains	448:476	In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent metalloporphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic microsensors control the permeation of lactate into the microsensors.
29043796	8	58	theme	lactate	1376:1382	arg1	sensors					1384:1390	fully implantable optical lactate sensors	1350:1390	fully implantable optical lactate sensors	1350:1390	These preliminary results support the premise that these composite hydrogels are capable of continuous lactate tracking and have the potential for use as fully implantable optical lactate sensors.
29043796	0	59	theme	Enzymatic	67:75	arg1	Sensing					85:91	Optical Enzymatic Lactate Sensing	59:91	Optical Enzymatic Lactate Sensing	59:91	Composite Hydrogels Containing Bioactive Microreactors for Optical Enzymatic Lactate Sensing.
29043796	7	60	theme	Repeated	1033:1040	arg1	exposure					1049:1056	Repeated cyclic exposure	1033:1056	Repeated cyclic exposure to high levels of lactate	1033:1082	Repeated cyclic exposure to high levels of lactate revealed that these sensors were extremely stable, with no significant loss in sensor response after 20 cycles.
29043796	1	61	theme	disease	178:184	arg1	status					186:191	disease status	178:191	disease status	178:191	Continuously monitoring specific biomarkers offer a promising method to interrogate disease status and progression.
29043796	2	62	used	used	300:303	arg2	platform					279:286	a composite hydrogel-based sensing platform	244:286	a composite hydrogel-based sensing platform that may be used for optical detection of lactate	244:336	In this work we have demonstrated a composite hydrogel-based sensing platform that may be used for optical detection of lactate.
28363875	4	0	theme	ethanol	816:822	arg1	QP					838:839	QP	838:839	QP	838:839	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	0	theme	ethanol	816:822	arg1	productivity					824:835	a very high ethanol productivity	804:835	a very high ethanol productivity (QP) of 7.94g/lh	804:852	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	5	1	dep	cells	898:902	arg1	ergosterol					969:978	ergosterol	969:978	ergosterol	969:978	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	1	dep	cells	898:902	arg1	acids					955:959	total fatty acids	943:959	total fatty acids (TFAs)	943:966	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	1	dep	cells	898:902	arg1	UFAs					936:939	UFAs	936:939	UFAs	936:939	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	1	dep	cells	898:902	arg1	acids					929:933	unsaturated fatty acids	911:933	unsaturated fatty acids (UFAs)	911:940	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	1	dep	cells	898:902	arg1	trehalose					984:992	trehalose	984:992	trehalose	984:992	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	2	theme	fermentation	1025:1036	arg1	efficiency					1038:1047	fermentation efficiency	1025:1047	fermentation efficiency	1025:1047	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	4	3	theme	high	811:814	arg1	QP					838:839	QP	838:839	QP	838:839	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	3	theme	high	811:814	arg1	productivity					824:835	a very high ethanol productivity	804:835	a very high ethanol productivity (QP) of 7.94g/lh	804:852	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	3	4	theme	optimum	491:497	arg1	0.25vvm					536:542	0.25vvm	536:542	0.25vvm	536:542	RSM results revealed that the optimum aeration rate and recycle ratio were 0.25vvm and 0.625, respectively.
28363875	3	4	theme	optimum	491:497	arg1	rate					508:511	the optimum aeration rate	487:511	the optimum aeration rate	487:511	RSM results revealed that the optimum aeration rate and recycle ratio were 0.25vvm and 0.625, respectively.
28363875	0	5	theme	cell	87:90	arg1	system					102:107	a cell recycling system	85:107	a cell recycling system	85:107	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice using a cell recycling system.
28363875	0	6	from	Improvement	0:10	arg1	juice					73:77	sweet sorghum stem juice	54:77	sweet sorghum stem juice	54:77	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice using a cell recycling system.
28363875	7	7	theme	stress	1284:1289	arg1	conditions					1291:1300	stress conditions	1284:1300	stress conditions	1284:1300	Additionally, high trehalose content was observed when the yeast was subjected to stress conditions.
28363875	1	8	dep	Saccharomyces	241:253	arg1	cerevisiae					255:264	cerevisiae	255:264	cerevisiae	255:264	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	4	9	theme	validation	573:582	arg1	experiment					584:593	The validation experiment	569:593	The validation experiment under the optimum conditions	569:622	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	6	10	theme	ethanol	1180:1186	arg1	fermentation					1188:1199	ethanol fermentation	1180:1199	ethanol fermentation	1180:1199	A higher ratio of UFAs/TFAs and ergosterol strongly promoted yeast viability and ethanol fermentation.
28363875	1	11	dep	variables	122:130	arg1	variables					122:130	The process variables	110:130	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice	110:300	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	11	dep	variables	122:130	arg1	rate					142:145	aeration rate	133:145	aeration rate	133:145	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	11	dep	variables	122:130	arg1	ratio					159:163	recycle ratio	151:163	recycle ratio	151:163	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	12	theme	response	323:330	arg1	RSM					353:355	RSM	353:355	RSM	353:355	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	12	theme	response	323:330	arg1	methodology					340:350	response surface methodology	323:350	response surface methodology (RSM)	323:356	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	5	13	theme	intracellular	859:871	arg1	related					1010:1016	related	1010:1016	related	1010:1016	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	13	theme	intracellular	859:871	arg1	composition					873:883	The intracellular composition	855:883	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose)	855:993	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	1	14	theme	recycle	151:157	arg1	variables					122:130	The process variables	110:130	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice	110:300	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	14	theme	recycle	151:157	arg1	ratio					159:163	recycle ratio	151:163	recycle ratio	151:163	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	0	15	theme	recycling	92:100	arg1	system					102:107	a cell recycling system	85:107	a cell recycling system	85:107	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice using a cell recycling system.
28363875	1	16	theme	surface	332:338	arg1	RSM					353:355	RSM	353:355	RSM	353:355	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	16	theme	surface	332:338	arg1	methodology					340:350	response surface methodology	323:350	response surface methodology (RSM)	323:356	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	3	17	theme	recycle	517:523	arg1	ratio					525:529	recycle ratio	517:529	recycle ratio	517:529	RSM results revealed that the optimum aeration rate and recycle ratio were 0.25vvm and 0.625, respectively.
28363875	5	18	theme	adaptive	1059:1066	arg1	response					1068:1075	yeast adaptive response	1053:1075	yeast adaptive response	1053:1075	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	6	19	theme	yeast	1160:1164	arg1	viability					1166:1174	yeast viability	1160:1174	yeast viability	1160:1174	A higher ratio of UFAs/TFAs and ergosterol strongly promoted yeast viability and ethanol fermentation.
28363875	2	20	theme	fermentation	414:425	arg1	efficiency					427:436	fermentation efficiency	414:436	fermentation efficiency	414:436	The relationship between intracellular composition and fermentation efficiency was also investigated.
28363875	6	21	theme	higher	1101:1106	arg1	ratio					1108:1112	A higher ratio	1099:1112	A higher ratio of UFAs/TFAs and ergosterol	1099:1140	A higher ratio of UFAs/TFAs and ergosterol strongly promoted yeast viability and ethanol fermentation.
28363875	0	22	theme	continuous	17:26	arg1	fermentation					36:47	a continuous ethanol fermentation	15:47	a continuous ethanol fermentation from sweet sorghum stem juice	15:77	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice using a cell recycling system.
28363875	1	23	theme	Saccharomyces	241:253	arg1	01					269:270	Saccharomyces cerevisiae NP 01	241:270	Saccharomyces cerevisiae NP 01	241:270	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	5	24	dep	efficiency	1038:1047	arg1	the					1021:1023	the	1021:1023	the	1021:1023	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	6	25	theme	UFAs/TFAs	1117:1125	arg1	ratio					1108:1112	A higher ratio	1099:1112	A higher ratio of UFAs/TFAs and ergosterol	1099:1140	A higher ratio of UFAs/TFAs and ergosterol strongly promoted yeast viability and ethanol fermentation.
28363875	4	26	theme	99.28g/l	734:741	arg1	productivity					824:835	a very high ethanol productivity	804:835	a very high ethanol productivity (QP) of 7.94g/lh	804:852	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	26	theme	99.28g/l	734:741	arg1	PE					727:728	PE	727:728	PE	727:728	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	26	theme	99.28g/l	734:741	arg1	concentration					712:724	an actual ethanol concentration	694:724	an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l),	694:798	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	26	theme	99.28g/l	734:741	arg1	QP					838:839	QP	838:839	QP	838:839	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	2	27	theme	intracellular	384:396	arg1	composition					398:408	intracellular composition	384:408	intracellular composition	384:408	The relationship between intracellular composition and fermentation efficiency was also investigated.
28363875	7	28	theme	high	1216:1219	arg1	content					1231:1237	high trehalose content	1216:1237	high trehalose content	1216:1237	Additionally, high trehalose content was observed when the yeast was subjected to stress conditions.
28363875	1	29	with	fermentation	190:201	arg1	CRS					233:235	CRS	233:235	CRS	233:235	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	29	with	fermentation	190:201	arg1	system					225:230	a cell recycling system	208:230	a cell recycling system (CRS)	208:236	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	5	30	theme	cells	898:902	arg1	related					1010:1016	related	1010:1016	related	1010:1016	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	30	theme	cells	898:902	arg1	composition					873:883	The intracellular composition	855:883	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose)	855:993	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	1	31	theme	continuous	171:180	arg1	fermentation					190:201	a continuous ethanol fermentation	169:201	a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01	169:270	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	0	32	theme	fermentation	36:47	arg1	Improvement					0:10	Improvement	0:10	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice	0:77	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice using a cell recycling system.
28363875	1	33	theme	NP	266:267	arg1	01					269:270	Saccharomyces cerevisiae NP 01	241:270	Saccharomyces cerevisiae NP 01	241:270	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	0	34	from	juice	73:77	arg1	fermentation					36:47	a continuous ethanol fermentation	15:47	a continuous ethanol fermentation from sweet sorghum stem juice	15:77	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice using a cell recycling system.
28363875	0	34	from	juice	73:77	arg1	Improvement					0:10	Improvement	0:10	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice	0:77	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice using a cell recycling system.
28363875	1	35	theme	ethanol	182:188	arg1	fermentation					190:201	a continuous ethanol fermentation	169:201	a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01	169:270	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	0	36	theme	ethanol	28:34	arg1	fermentation					36:47	a continuous ethanol fermentation	15:47	a continuous ethanol fermentation from sweet sorghum stem juice	15:77	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice using a cell recycling system.
28363875	4	37	theme	optimum	605:611	arg1	conditions					613:622	the optimum conditions	601:622	the optimum conditions	601:622	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	1	38	theme	fermentation	190:201	arg1	variables					122:130	The process variables	110:130	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice	110:300	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	38	theme	fermentation	190:201	arg1	rate					142:145	aeration rate	133:145	aeration rate	133:145	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	38	theme	fermentation	190:201	arg1	ratio					159:163	recycle ratio	151:163	recycle ratio	151:163	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	0	39	theme	sweet	54:58	arg1	juice					73:77	sweet sorghum stem juice	54:77	sweet sorghum stem juice	54:77	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice using a cell recycling system.
28363875	5	40	theme	yeast	1053:1057	arg1	response					1068:1075	yeast adaptive response	1053:1075	yeast adaptive response	1053:1075	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	41	theme	ethanol	1083:1089	arg1	stress					1091:1096	ethanol stress	1083:1096	ethanol stress	1083:1096	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	42	theme	total	943:947	arg1	TFAs					962:965	TFAs	962:965	TFAs	962:965	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	42	theme	total	943:947	arg1	acids					955:959	total fatty acids	943:959	total fatty acids (TFAs)	943:966	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	42	theme	total	943:947	arg1	acids					929:933	unsaturated fatty acids	911:933	unsaturated fatty acids (UFAs)	911:940	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	4	43	theme	ethanol	704:710	arg1	PE					727:728	PE	727:728	PE	727:728	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	43	theme	ethanol	704:710	arg1	concentration					712:724	an actual ethanol concentration	694:724	an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l),	694:798	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	1	44	from	juice	296:300	arg1	variables					122:130	The process variables	110:130	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice	110:300	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	44	from	juice	296:300	arg1	rate					142:145	aeration rate	133:145	aeration rate	133:145	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	44	from	juice	296:300	arg1	ratio					159:163	recycle ratio	151:163	recycle ratio	151:163	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	45	theme	sweet	277:281	arg1	juice					296:300	sweet sorghum stem juice	277:300	sweet sorghum stem juice	277:300	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	5	46	theme	fatty	949:953	arg1	TFAs					962:965	TFAs	962:965	TFAs	962:965	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	46	theme	fatty	949:953	arg1	acids					955:959	total fatty acids	943:959	total fatty acids (TFAs)	943:966	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	46	theme	fatty	949:953	arg1	acids					929:933	unsaturated fatty acids	911:933	unsaturated fatty acids (UFAs)	911:940	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	4	47	theme	actual	697:702	arg1	PE					727:728	PE	727:728	PE	727:728	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	47	theme	actual	697:702	arg1	concentration					712:724	an actual ethanol concentration	694:724	an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l),	694:798	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	1	48	theme	sorghum	283:289	arg1	juice					296:300	sweet sorghum stem juice	277:300	sweet sorghum stem juice	277:300	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	4	49	theme	7.94g/lh	845:852	arg1	productivity					824:835	a very high ethanol productivity	804:835	a very high ethanol productivity (QP) of 7.94g/lh	804:852	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	49	theme	7.94g/lh	845:852	arg1	PE					727:728	PE	727:728	PE	727:728	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	49	theme	7.94g/lh	845:852	arg1	concentration					712:724	an actual ethanol concentration	694:724	an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l),	694:798	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	49	theme	7.94g/lh	845:852	arg1	QP					838:839	QP	838:839	QP	838:839	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	5	50	theme	fatty	923:927	arg1	ergosterol					969:978	ergosterol	969:978	ergosterol	969:978	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	50	theme	fatty	923:927	arg1	acids					955:959	total fatty acids	943:959	total fatty acids (TFAs)	943:966	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	50	theme	fatty	923:927	arg1	UFAs					936:939	UFAs	936:939	UFAs	936:939	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	50	theme	fatty	923:927	arg1	acids					929:933	unsaturated fatty acids	911:933	unsaturated fatty acids (UFAs)	911:940	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	50	theme	fatty	923:927	arg1	trehalose					984:992	trehalose	984:992	trehalose	984:992	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	6	51	theme	ergosterol	1131:1140	arg1	ratio					1108:1112	A higher ratio	1099:1112	A higher ratio of UFAs/TFAs and ergosterol	1099:1140	A higher ratio of UFAs/TFAs and ergosterol strongly promoted yeast viability and ethanol fermentation.
28363875	1	52	theme	process	114:120	arg1	variables					122:130	The process variables	110:130	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice	110:300	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	52	theme	process	114:120	arg1	rate					142:145	aeration rate	133:145	aeration rate	133:145	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	52	theme	process	114:120	arg1	ratio					159:163	recycle ratio	151:163	recycle ratio	151:163	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	5	53	theme	yeast	892:896	arg1	cells					898:902	the yeast cells	888:902	the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose)	888:993	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	54	dep	acids	929:933	arg1	i.e.					905:908	i.e.	905:908	i.e.	905:908	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	55	theme	unsaturated	911:921	arg1	ergosterol					969:978	ergosterol	969:978	ergosterol	969:978	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	55	theme	unsaturated	911:921	arg1	acids					955:959	total fatty acids	943:959	total fatty acids (TFAs)	943:966	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	55	theme	unsaturated	911:921	arg1	UFAs					936:939	UFAs	936:939	UFAs	936:939	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	55	theme	unsaturated	911:921	arg1	acids					929:933	unsaturated fatty acids	911:933	unsaturated fatty acids (UFAs)	911:940	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	5	55	theme	unsaturated	911:921	arg1	trehalose					984:992	trehalose	984:992	trehalose	984:992	The intracellular composition of the yeast cells (i.e., unsaturated fatty acids (UFAs), total fatty acids (TFAs), ergosterol and trehalose) was positively related to the fermentation efficiency and yeast adaptive response under ethanol stress.
28363875	4	56	theme	experiment	672:681	arg1	reliability					653:663	reliability	653:663	reliability	653:663	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	56	theme	experiment	672:681	arg1	precision					639:647	high precision	634:647	high precision	634:647	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	3	57	theme	RSM	461:463	arg1	results					465:471	RSM results	461:471	RSM results	461:471	RSM results revealed that the optimum aeration rate and recycle ratio were 0.25vvm and 0.625, respectively.
28363875	0	58	theme	stem	68:71	arg1	juice					73:77	sweet sorghum stem juice	54:77	sweet sorghum stem juice	54:77	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice using a cell recycling system.
28363875	4	59	theme	high	634:637	arg1	precision					639:647	high precision	634:647	high precision	634:647	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	60	theme	predicted	772:780	arg1	99.28g/l					734:741	99.28g/l	734:741	99.28g/l	734:741	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	60	theme	predicted	772:780	arg1	98.01g/l					789:796	98.01g/l	789:796	98.01g/l	789:796	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	4	60	theme	predicted	772:780	arg1	value					782:786	the predicted value	768:786	the predicted value (98.01g/l)	768:797	The validation experiment under the optimum conditions indicated high precision and reliability of the experiment, achieving an actual ethanol concentration (PE) of 99.28g/l, which was very close to the predicted value (98.01g/l), and a very high ethanol productivity (QP) of 7.94g/lh.
28363875	7	61	theme	trehalose	1221:1229	arg1	content					1231:1237	high trehalose content	1216:1237	high trehalose content	1216:1237	Additionally, high trehalose content was observed when the yeast was subjected to stress conditions.
28363875	3	62	theme	aeration	499:506	arg1	0.25vvm					536:542	0.25vvm	536:542	0.25vvm	536:542	RSM results revealed that the optimum aeration rate and recycle ratio were 0.25vvm and 0.625, respectively.
28363875	3	62	theme	aeration	499:506	arg1	rate					508:511	the optimum aeration rate	487:511	the optimum aeration rate	487:511	RSM results revealed that the optimum aeration rate and recycle ratio were 0.25vvm and 0.625, respectively.
28363875	1	63	theme	cell	210:213	arg1	CRS					233:235	CRS	233:235	CRS	233:235	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	63	theme	cell	210:213	arg1	system					225:230	a cell recycling system	208:230	a cell recycling system (CRS)	208:236	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	0	64	theme	sorghum	60:66	arg1	juice					73:77	sweet sorghum stem juice	54:77	sweet sorghum stem juice	54:77	Improvement of a continuous ethanol fermentation from sweet sorghum stem juice using a cell recycling system.
28363875	1	65	theme	stem	291:294	arg1	juice					296:300	sweet sorghum stem juice	277:300	sweet sorghum stem juice	277:300	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	66	theme	aeration	133:140	arg1	variables					122:130	The process variables	110:130	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice	110:300	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	66	theme	aeration	133:140	arg1	rate					142:145	aeration rate	133:145	aeration rate	133:145	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	67	theme	recycling	215:223	arg1	CRS					233:235	CRS	233:235	CRS	233:235	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
28363875	1	67	theme	recycling	215:223	arg1	system					225:230	a cell recycling system	208:230	a cell recycling system (CRS)	208:236	The process variables (aeration rate and recycle ratio) of a continuous ethanol fermentation with a cell recycling system (CRS) by Saccharomyces cerevisiae NP 01 from sweet sorghum stem juice were optimized using response surface methodology (RSM).
27612753	4	0	theme	composite	984:992	arg1	film					994:997	the composite film	980:997	the composite film	980:997	The incorporation of chitosan and PEG into gelatin showed improved mechanical properties of both TS and Eb, which suggested the occurrence of interaction among gelatin, chitosan and PEG molecules in the composite film.
27612753	3	1	theme	aging	718:722	arg1	test					724:727	aging test	718:727	aging test	718:727	Suitable packaging was also selected and stability study and aging test of the composite film were performed after packing.
27612753	9	2	theme	dressing	1536:1543	arg1	material					1545:1552	an ideal wound dressing material	1521:1552	an ideal wound dressing material	1521:1552	Based on the studies related to the performance with confirmed identity, we concluded that our developed bi-layer film is very potential as an ideal wound dressing material.
27612753	2	3	from	capacity	626:633	arg1	TS					488:489	TS	488:489	TS	488:489	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	3	from	capacity	626:633	arg1	elongation					496:505	elongation	496:505	elongation	496:505	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	3	from	capacity	626:633	arg1	strength					478:485	tensile strength	470:485	tensile strength (TS)	470:490	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	7	4	theme	water	1209:1213	arg1	studies					1222:1228	water uptake studies	1209:1228	water uptake studies	1209:1228	On the other hand, water uptake studies suggested excellent fluid drainage capability and hydro-stability of the composite film.
27612753	3	5	theme	stability	698:706	arg1	study					708:712	stability study	698:712	stability study	698:712	Suitable packaging was also selected and stability study and aging test of the composite film were performed after packing.
27612753	4	6	theme	TS	878:879	arg1	properties					859:868	improved mechanical properties	839:868	improved mechanical properties	839:868	The incorporation of chitosan and PEG into gelatin showed improved mechanical properties of both TS and Eb, which suggested the occurrence of interaction among gelatin, chitosan and PEG molecules in the composite film.
27612753	1	7	theme	polyvinyl	181:189	arg1	PVA					200:202	PVA	200:202	PVA	200:202	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	1	7	theme	polyvinyl	181:189	arg1	alcohol					191:197	polyvinyl alcohol	181:197	polyvinyl alcohol (PVA)	181:203	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	2	8	from	Fourier	563:569	arg1	TS					488:489	TS	488:489	TS	488:489	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	8	from	Fourier	563:569	arg1	elongation					496:505	elongation	496:505	elongation	496:505	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	8	from	Fourier	563:569	arg1	strength					478:485	tensile strength	470:485	tensile strength (TS)	470:490	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	7	9	theme	other	1197:1201	arg1	hand					1203:1206	the other hand	1193:1206	the other hand	1193:1206	On the other hand, water uptake studies suggested excellent fluid drainage capability and hydro-stability of the composite film.
27612753	9	10	theme	wound	1530:1534	arg1	material					1545:1552	an ideal wound dressing material	1521:1552	an ideal wound dressing material	1521:1552	Based on the studies related to the performance with confirmed identity, we concluded that our developed bi-layer film is very potential as an ideal wound dressing material.
27612753	4	11	theme	improved	839:846	arg1	properties					859:868	improved mechanical properties	839:868	improved mechanical properties	839:868	The incorporation of chitosan and PEG into gelatin showed improved mechanical properties of both TS and Eb, which suggested the occurrence of interaction among gelatin, chitosan and PEG molecules in the composite film.
27612753	8	12	theme	excellent	1353:1361	arg1	biocompatibility					1363:1378	excellent biocompatibility	1353:1378	excellent biocompatibility	1353:1378	The proposed dressing also showed excellent biocompatibility.
27612753	1	13	theme	biomedical	276:285	arg1	applications					287:298	biomedical applications	276:298	biomedical applications especially as an alternative of advanced tissue scaffold	276:355	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	2	14	theme	scanning	535:542	arg1	DSC					557:559	DSC	557:559	DSC	557:559	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	14	theme	scanning	535:542	arg1	calorimetry					544:554	differential scanning calorimetry	522:554	differential scanning calorimetry (DSC)	522:560	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	6	15	theme	increased	1142:1150	arg1	stability					1160:1168	increased thermal stability	1142:1168	increased thermal stability	1142:1168	Results from the DSC study suggested increased thermal stability after crosslinking.
27612753	4	16	theme	Eb	885:886	arg1	properties					859:868	improved mechanical properties	839:868	improved mechanical properties	839:868	The incorporation of chitosan and PEG into gelatin showed improved mechanical properties of both TS and Eb, which suggested the occurrence of interaction among gelatin, chitosan and PEG molecules in the composite film.
27612753	9	17	with	performance	1417:1427	arg1	identity					1444:1451	confirmed identity	1434:1451	confirmed identity	1434:1451	Based on the studies related to the performance with confirmed identity, we concluded that our developed bi-layer film is very potential as an ideal wound dressing material.
27612753	2	18	theme	differential	522:533	arg1	DSC					557:559	DSC	557:559	DSC	557:559	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	18	theme	differential	522:533	arg1	calorimetry					544:554	differential scanning calorimetry	522:554	differential scanning calorimetry (DSC)	522:560	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	3	19	theme	composite	736:744	arg1	film					746:749	the composite film	732:749	the composite film	732:749	Suitable packaging was also selected and stability study and aging test of the composite film were performed after packing.
27612753	2	20	from	biocompatibility	639:654	arg1	TS					488:489	TS	488:489	TS	488:489	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	20	from	biocompatibility	639:654	arg1	elongation					496:505	elongation	496:505	elongation	496:505	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	20	from	biocompatibility	639:654	arg1	strength					478:485	tensile strength	470:485	tensile strength (TS)	470:490	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	9	21	theme	developed	1476:1484	arg1	film					1495:1498	our developed bi-layer film	1472:1498	our developed bi-layer film	1472:1498	Based on the studies related to the performance with confirmed identity, we concluded that our developed bi-layer film is very potential as an ideal wound dressing material.
27612753	4	22	theme	mechanical	848:857	arg1	properties					859:868	improved mechanical properties	839:868	improved mechanical properties	839:868	The incorporation of chitosan and PEG into gelatin showed improved mechanical properties of both TS and Eb, which suggested the occurrence of interaction among gelatin, chitosan and PEG molecules in the composite film.
27612753	1	23	theme	Bio-active	93:102	arg1	film					118:121	Bio-active bi-layer thin film	93:121	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend	93:256	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	2	24	theme	thermal	416:422	arg1	characterization					445:460	mechanical, thermal and physico-chemical characterization	404:460	mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility	404:654	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	6	25	theme	thermal	1152:1158	arg1	stability					1160:1168	increased thermal stability	1142:1168	increased thermal stability	1142:1168	Results from the DSC study suggested increased thermal stability after crosslinking.
27612753	2	26	theme	drainage	617:624	arg1	capacity					626:633	fluid drainage capacity	611:633	fluid drainage capacity	611:633	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	1	27	theme	bi-layer	104:111	arg1	film					118:121	Bio-active bi-layer thin film	93:121	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend	93:256	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	5	28	theme	FTIR	1093:1096	arg1	study					1098:1102	FTIR study	1093:1102	FTIR study	1093:1102	The presence of crosslinking as an interaction of above three polymers was also confirmed by FTIR study.
27612753	9	29	theme	bi-layer	1486:1493	arg1	film					1495:1498	our developed bi-layer film	1472:1498	our developed bi-layer film	1472:1498	Based on the studies related to the performance with confirmed identity, we concluded that our developed bi-layer film is very potential as an ideal wound dressing material.
27612753	2	30	theme	fluid	611:615	arg1	capacity					626:633	fluid drainage capacity	611:633	fluid drainage capacity	611:633	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	1	31	theme	thin	113:116	arg1	film					118:121	Bio-active bi-layer thin film	93:121	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend	93:256	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	2	32	theme	mechanical	404:413	arg1	characterization					445:460	mechanical, thermal and physico-chemical characterization	404:460	mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility	404:654	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	0	33	theme	Bi-layer	28:35	arg1	dressing					37:44	hydrocolloid Bi-layer dressing	15:44	hydrocolloid Bi-layer dressing	15:44	Development of hydrocolloid Bi-layer dressing with bio-adhesive and non-adhesive properties.
27612753	4	34	theme	interaction	923:933	arg1	occurrence					909:918	the occurrence	905:918	the occurrence of interaction among gelatin, chitosan and PEG molecules in the composite film	905:997	The incorporation of chitosan and PEG into gelatin showed improved mechanical properties of both TS and Eb, which suggested the occurrence of interaction among gelatin, chitosan and PEG molecules in the composite film.
27612753	6	35	from	study	1126:1130	arg1	Results					1105:1111	Results	1105:1111	Results from the DSC study	1105:1130	Results from the DSC study suggested increased thermal stability after crosslinking.
27612753	7	36	theme	film	1313:1316	arg1	hydro-stability					1280:1294	hydro-stability	1280:1294	hydro-stability	1280:1294	On the other hand, water uptake studies suggested excellent fluid drainage capability and hydro-stability of the composite film.
27612753	7	36	theme	film	1313:1316	arg1	capability					1265:1274	excellent fluid drainage capability	1240:1274	excellent fluid drainage capability	1240:1274	On the other hand, water uptake studies suggested excellent fluid drainage capability and hydro-stability of the composite film.
27612753	0	37	theme	hydrocolloid	15:26	arg1	dressing					37:44	hydrocolloid Bi-layer dressing	15:44	hydrocolloid Bi-layer dressing	15:44	Development of hydrocolloid Bi-layer dressing with bio-adhesive and non-adhesive properties.
27612753	3	38	theme	film	746:749	arg1	study					708:712	stability study	698:712	stability study	698:712	Suitable packaging was also selected and stability study and aging test of the composite film were performed after packing.
27612753	3	38	theme	film	746:749	arg1	test					724:727	aging test	718:727	aging test	718:727	Suitable packaging was also selected and stability study and aging test of the composite film were performed after packing.
27612753	3	39	theme	Suitable	657:664	arg1	packaging					666:674	Suitable packaging	657:674	Suitable packaging	657:674	Suitable packaging was also selected and stability study and aging test of the composite film were performed after packing.
27612753	7	40	theme	excellent	1240:1248	arg1	capability					1265:1274	excellent fluid drainage capability	1240:1274	excellent fluid drainage capability	1240:1274	On the other hand, water uptake studies suggested excellent fluid drainage capability and hydro-stability of the composite film.
27612753	1	41	theme	gelatin/chitosan/polyethylene	209:237	arg1	PEG					247:249	PEG	247:249	PEG	247:249	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	1	41	theme	gelatin/chitosan/polyethylene	209:237	arg1	glycol					239:244	gelatin/chitosan/polyethylene glycol	209:244	gelatin/chitosan/polyethylene glycol (PEG)	209:250	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	2	42	theme	developed	362:370	arg1	film					382:385	The developed composite film	358:385	The developed composite film	358:385	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	43	from	break	510:514	arg1	TS					488:489	TS	488:489	TS	488:489	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	43	from	break	510:514	arg1	elongation					496:505	elongation	496:505	elongation	496:505	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	43	from	break	510:514	arg1	strength					478:485	tensile strength	470:485	tensile strength (TS)	470:490	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	0	44	theme	dressing	37:44	arg1	Development					0:10	Development	0:10	Development of hydrocolloid Bi-layer dressing with bio-adhesive and non-adhesive properties.	0:91	Development of hydrocolloid Bi-layer dressing with bio-adhesive and non-adhesive properties.
27612753	1	45	contain	having	123:128	arg1	film					118:121	Bio-active bi-layer thin film	93:121	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend	93:256	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	1	45	contain	having	123:128	arg2	end					165:167	both bio-adhesive and non-adhesive end	130:167	both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend	130:256	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	9	46	theme	confirmed	1434:1442	arg1	identity					1444:1451	confirmed identity	1434:1451	confirmed identity	1434:1451	Based on the studies related to the performance with confirmed identity, we concluded that our developed bi-layer film is very potential as an ideal wound dressing material.
27612753	5	47	theme	crosslinking	1016:1027	arg1	presence					1004:1011	The presence	1000:1011	The presence of crosslinking as an interaction of above three polymers	1000:1069	The presence of crosslinking as an interaction of above three polymers was also confirmed by FTIR study.
27612753	2	48	theme	physico-chemical	428:443	arg1	characterization					445:460	mechanical, thermal and physico-chemical characterization	404:460	mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility	404:654	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	5	49	theme	polymers	1062:1069	arg1	interaction					1035:1045	an interaction	1032:1045	an interaction of above three polymers	1032:1069	The presence of crosslinking as an interaction of above three polymers was also confirmed by FTIR study.
27612753	1	50	theme	advanced	332:339	arg1	scaffold					348:355	advanced tissue scaffold	332:355	advanced tissue scaffold	332:355	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	2	51	dep	Fourier	563:569	arg1	transform					571:579	transform	571:579	transform infrared spectroscopy (FTIR)	571:608	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	1	52	dep	alcohol	191:197	arg1	blend					252:256	blend	252:256	blend	252:256	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	2	53	theme	composite	372:380	arg1	film					382:385	The developed composite film	358:385	The developed composite film	358:385	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	0	54	theme	bio-adhesive	51:62	arg1	properties					81:90	bio-adhesive and non-adhesive properties	51:90	bio-adhesive and non-adhesive properties	51:90	Development of hydrocolloid Bi-layer dressing with bio-adhesive and non-adhesive properties.
27612753	1	55	theme	tissue	341:346	arg1	scaffold					348:355	advanced tissue scaffold	332:355	advanced tissue scaffold	332:355	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	2	56	theme	infrared	581:588	arg1	FTIR					604:607	FTIR	604:607	FTIR	604:607	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	56	theme	infrared	581:588	arg1	spectroscopy					590:601	infrared spectroscopy	581:601	infrared spectroscopy (FTIR)	581:608	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	4	57	from	occurrence	909:918	arg1	film					994:997	the composite film	980:997	the composite film	980:997	The incorporation of chitosan and PEG into gelatin showed improved mechanical properties of both TS and Eb, which suggested the occurrence of interaction among gelatin, chitosan and PEG molecules in the composite film.
27612753	1	58	theme	bio-adhesive	135:146	arg1	end					165:167	both bio-adhesive and non-adhesive end	130:167	both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend	130:256	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	4	59	theme	PEG	963:965	arg1	molecules					967:975	PEG molecules	963:975	PEG molecules	963:975	The incorporation of chitosan and PEG into gelatin showed improved mechanical properties of both TS and Eb, which suggested the occurrence of interaction among gelatin, chitosan and PEG molecules in the composite film.
27612753	1	60	theme	scaffold	348:355	arg1	alternative					317:327	an alternative	314:327	an alternative of advanced tissue scaffold	314:355	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	4	61	theme	chitosan	802:809	arg1	incorporation					785:797	The incorporation	781:797	The incorporation of chitosan and PEG into gelatin	781:830	The incorporation of chitosan and PEG into gelatin showed improved mechanical properties of both TS and Eb, which suggested the occurrence of interaction among gelatin, chitosan and PEG molecules in the composite film.
27612753	0	62	with	Development	0:10	arg1	properties					81:90	bio-adhesive and non-adhesive properties	51:90	bio-adhesive and non-adhesive properties	51:90	Development of hydrocolloid Bi-layer dressing with bio-adhesive and non-adhesive properties.
27612753	9	63	theme	related	1402:1408	arg1	studies					1394:1400	the studies	1390:1400	the studies related to the performance with confirmed identity	1390:1451	Based on the studies related to the performance with confirmed identity, we concluded that our developed bi-layer film is very potential as an ideal wound dressing material.
27612753	2	64	from	calorimetry	544:554	arg1	TS					488:489	TS	488:489	TS	488:489	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	64	from	calorimetry	544:554	arg1	elongation					496:505	elongation	496:505	elongation	496:505	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	64	from	calorimetry	544:554	arg1	strength					478:485	tensile strength	470:485	tensile strength (TS)	470:490	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	8	65	theme	proposed	1323:1330	arg1	dressing					1332:1339	The proposed dressing	1319:1339	The proposed dressing	1319:1339	The proposed dressing also showed excellent biocompatibility.
27612753	1	66	theme	non-adhesive	152:163	arg1	end					165:167	both bio-adhesive and non-adhesive end	130:167	both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend	130:256	Bio-active bi-layer thin film having both bio-adhesive and non-adhesive end composed of polyvinyl alcohol (PVA) and gelatin/chitosan/polyethylene glycol (PEG) blend was developed for biomedical applications especially as an alternative of advanced tissue scaffold.
27612753	6	67	theme	DSC	1122:1124	arg1	study					1126:1130	the DSC study	1118:1130	the DSC study	1118:1130	Results from the DSC study suggested increased thermal stability after crosslinking.
27612753	7	68	theme	uptake	1215:1220	arg1	studies					1222:1228	water uptake studies	1209:1228	water uptake studies	1209:1228	On the other hand, water uptake studies suggested excellent fluid drainage capability and hydro-stability of the composite film.
27612753	7	69	theme	composite	1303:1311	arg1	film					1313:1316	the composite film	1299:1316	the composite film	1299:1316	On the other hand, water uptake studies suggested excellent fluid drainage capability and hydro-stability of the composite film.
27612753	4	70	theme	PEG	815:817	arg1	incorporation					785:797	The incorporation	781:797	The incorporation of chitosan and PEG into gelatin	781:830	The incorporation of chitosan and PEG into gelatin showed improved mechanical properties of both TS and Eb, which suggested the occurrence of interaction among gelatin, chitosan and PEG molecules in the composite film.
27612753	9	71	theme	ideal	1524:1528	arg1	material					1545:1552	an ideal wound dressing material	1521:1552	an ideal wound dressing material	1521:1552	Based on the studies related to the performance with confirmed identity, we concluded that our developed bi-layer film is very potential as an ideal wound dressing material.
27612753	7	72	theme	fluid	1250:1254	arg1	capability					1265:1274	excellent fluid drainage capability	1240:1274	excellent fluid drainage capability	1240:1274	On the other hand, water uptake studies suggested excellent fluid drainage capability and hydro-stability of the composite film.
27612753	0	73	theme	non-adhesive	68:79	arg1	properties					81:90	bio-adhesive and non-adhesive properties	51:90	bio-adhesive and non-adhesive properties	51:90	Development of hydrocolloid Bi-layer dressing with bio-adhesive and non-adhesive properties.
27612753	2	74	theme	tensile	470:476	arg1	TS					488:489	TS	488:489	TS	488:489	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	2	74	theme	tensile	470:476	arg1	strength					478:485	tensile strength	470:485	tensile strength (TS)	470:490	The developed composite film was subjected to mechanical, thermal and physico-chemical characterization such as tensile strength (TS) and elongation at break (Eb), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), fluid drainage capacity and biocompatibility.
27612753	7	75	theme	drainage	1256:1263	arg1	capability					1265:1274	excellent fluid drainage capability	1240:1274	excellent fluid drainage capability	1240:1274	On the other hand, water uptake studies suggested excellent fluid drainage capability and hydro-stability of the composite film.
25504195	5	0	dep	terrestrial	848:858	arg1	humic-like					861:870	humic-like	861:870	humic-like	861:870	The analyses revealed significant differences in optical properties between sampling methods, with the passive samplers preferentially collecting terrestrial, humic-like DOM.
25504195	3	1	theme	passive	486:492	arg1	samplers					494:501	the passive samplers	482:501	the passive samplers	482:501	Two deployments of the passive samplers were conducted, during which grab samples were frequently collected for comparison.
25504195	0	2	theme	matter	71:76	arg1	collection					39:48	the collection	35:48	the collection of dissolved organic matter in streams	35:87	Evaluation of passive samplers for the collection of dissolved organic matter in streams.
25504195	6	3	theme	sampling	937:944	arg1	method					946:951	each sampling method	932:951	each sampling method	932:951	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	2	4	theme	DEAE	352:355	arg1	medium					382:387	a sampling medium	371:387	a sampling medium	371:387	Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana.
25504195	2	4	theme	DEAE	352:355	arg1	cellulose					358:366	diethylaminoethyl (DEAE) cellulose	333:366	diethylaminoethyl (DEAE) cellulose	333:366	Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana.
25504195	6	5	theme	DEAE	1023:1026	arg1	cellulose					1028:1036	the DEAE cellulose	1019:1036	the DEAE cellulose in the passive samplers	1019:1060	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	4	6	from	Differences	587:597	arg1	quality					606:612	DOM quality	602:612	DOM quality	602:612	Differences in DOM quality between sites and sampling methods were assessed using several common optical analyses.
25504195	6	7	from	differences	896:906	arg1	composition					915:925	DOM composition	911:925	DOM composition from each sampling method	911:951	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	6	7	from	differences	896:906	arg1	method					946:951	each sampling method	932:951	each sampling method	932:951	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	1	8	from	matter	141:146	arg1	streams					157:163	streams	157:163	streams	157:163	Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints.
25504195	3	9	theme	grab	532:535	arg1	samples					537:543	grab samples	532:543	grab samples	532:543	Two deployments of the passive samplers were conducted, during which grab samples were frequently collected for comparison.
25504195	5	10	theme	optical	751:757	arg1	properties					759:768	optical properties	751:768	optical properties	751:768	The analyses revealed significant differences in optical properties between sampling methods, with the passive samplers preferentially collecting terrestrial, humic-like DOM.
25504195	0	11	from	collection	39:48	arg1	streams					81:87	streams	81:87	streams	81:87	Evaluation of passive samplers for the collection of dissolved organic matter in streams.
25504195	4	12	theme	several	669:675	arg1	analyses					692:699	several common optical analyses	669:699	several common optical analyses	669:699	Differences in DOM quality between sites and sampling methods were assessed using several common optical analyses.
25504195	6	13	theme	DOM	911:913	arg1	composition					915:925	DOM composition	911:925	DOM composition from each sampling method	911:951	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	6	14	theme	passive	1045:1051	arg1	samplers					1053:1060	the passive samplers	1041:1060	the passive samplers	1041:1060	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	2	15	theme	stream	436:441	arg1	network					443:449	a temperate stream network	424:449	a temperate stream network in Indiana	424:460	Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana.
25504195	6	16	from	method	946:951	arg1	composition					915:925	DOM composition	911:925	DOM composition from each sampling method	911:951	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	6	16	from	method	946:951	arg1	differences					896:906	the differences	892:906	the differences in DOM composition from each sampling method	892:951	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	6	17	from	cellulose	1028:1036	arg1	samplers					1053:1060	the passive samplers	1041:1060	the passive samplers	1041:1060	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	2	18	from	network	443:449	arg1	Indiana					454:460	Indiana	454:460	Indiana	454:460	Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana.
25504195	2	19	theme	sampling	373:380	arg1	medium					382:387	a sampling medium	371:387	a sampling medium	371:387	Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana.
25504195	2	19	theme	sampling	373:380	arg1	cellulose					358:366	diethylaminoethyl (DEAE) cellulose	333:366	diethylaminoethyl (DEAE) cellulose	333:366	Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana.
25504195	2	20	theme	temperate	426:434	arg1	network					443:449	a temperate stream network	424:449	a temperate stream network in Indiana	424:460	Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana.
25504195	6	21	theme	compounds	1006:1014	arg1	binding					981:987	preferential binding	968:987	preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers	968:1060	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	7	22	theme	cost-effective	1111:1124	arg1	sample					1138:1143	a cost-effective, integrated sample	1109:1143	a cost-effective, integrated sample of DOM	1109:1150	Nonetheless, the passive samplers may provide a cost-effective, integrated sample of DOM in situations where the bulk DOM pool is composed mainly of terrestrial, humic-like compounds.
25504195	6	23	dep	caused	958:963	arg1	We					877:878	We	877:878	We	877:878	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	0	24	theme	samplers	22:29	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of passive samplers for the collection of dissolved organic matter in streams.	0:88	Evaluation of passive samplers for the collection of dissolved organic matter in streams.
25504195	6	25	theme	humic	1000:1004	arg1	compounds					1006:1014	complex humic compounds	992:1014	complex humic compounds	992:1014	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	7	26	theme	DOM	1148:1150	arg1	sample					1138:1143	a cost-effective, integrated sample	1109:1143	a cost-effective, integrated sample of DOM	1109:1150	Nonetheless, the passive samplers may provide a cost-effective, integrated sample of DOM in situations where the bulk DOM pool is composed mainly of terrestrial, humic-like compounds.
25504195	1	27	theme	Traditional	90:100	arg1	methods					111:117	Traditional sampling methods	90:117	Traditional sampling methods for dissolved organic matter (DOM) in streams	90:163	Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints.
25504195	0	28	theme	passive	14:20	arg1	samplers					22:29	passive samplers	14:29	passive samplers	14:29	Evaluation of passive samplers for the collection of dissolved organic matter in streams.
25504195	6	29	theme	complex	992:998	arg1	compounds					1006:1014	complex humic compounds	992:1014	complex humic compounds	992:1014	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	1	30	theme	sampling	102:109	arg1	methods					111:117	Traditional sampling methods	90:117	Traditional sampling methods for dissolved organic matter (DOM) in streams	90:163	Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints.
25504195	1	31	theme	long-term	189:197	arg1	studies					199:205	long-term studies	189:205	long-term studies	189:205	Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints.
25504195	7	32	theme	terrestrial	1212:1222	arg1	compounds					1236:1244	terrestrial, humic-like compounds	1212:1244	terrestrial, humic-like compounds	1212:1244	Nonetheless, the passive samplers may provide a cost-effective, integrated sample of DOM in situations where the bulk DOM pool is composed mainly of terrestrial, humic-like compounds.
25504195	7	33	theme	passive	1080:1086	arg1	samplers					1088:1095	the passive samplers	1076:1095	the passive samplers	1076:1095	Nonetheless, the passive samplers may provide a cost-effective, integrated sample of DOM in situations where the bulk DOM pool is composed mainly of terrestrial, humic-like compounds.
25504195	0	34	from	matter	71:76	arg1	streams					81:87	streams	81:87	streams	81:87	Evaluation of passive samplers for the collection of dissolved organic matter in streams.
25504195	7	35	theme	bulk	1176:1179	arg1	pool					1185:1188	the bulk DOM pool	1172:1188	the bulk DOM pool	1172:1188	Nonetheless, the passive samplers may provide a cost-effective, integrated sample of DOM in situations where the bulk DOM pool is composed mainly of terrestrial, humic-like compounds.
25504195	5	36	theme	significant	724:734	arg1	differences					736:746	significant differences	724:746	significant differences in optical properties between sampling methods	724:793	The analyses revealed significant differences in optical properties between sampling methods, with the passive samplers preferentially collecting terrestrial, humic-like DOM.
25504195	5	37	from	differences	736:746	arg1	properties					759:768	optical properties	751:768	optical properties	751:768	The analyses revealed significant differences in optical properties between sampling methods, with the passive samplers preferentially collecting terrestrial, humic-like DOM.
25504195	5	38	theme	terrestrial	848:858	arg1	DOM					872:874	terrestrial, humic-like DOM	848:874	terrestrial, humic-like DOM	848:874	The analyses revealed significant differences in optical properties between sampling methods, with the passive samplers preferentially collecting terrestrial, humic-like DOM.
25504195	4	39	theme	sampling	632:639	arg1	methods					641:647	sampling methods	632:647	sampling methods	632:647	Differences in DOM quality between sites and sampling methods were assessed using several common optical analyses.
25504195	5	40	theme	passive	805:811	arg1	samplers					813:820	the passive samplers	801:820	the passive samplers preferentially collecting terrestrial, humic-like DOM	801:874	The analyses revealed significant differences in optical properties between sampling methods, with the passive samplers preferentially collecting terrestrial, humic-like DOM.
25504195	6	41	theme	preferential	968:979	arg1	binding					981:987	preferential binding	968:987	preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers	968:1060	We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers.
25504195	5	42	theme	sampling	778:785	arg1	methods					787:793	sampling methods	778:793	sampling methods	778:793	The analyses revealed significant differences in optical properties between sampling methods, with the passive samplers preferentially collecting terrestrial, humic-like DOM.
25504195	3	43	theme	samplers	494:501	arg1	deployments					467:477	Two deployments	463:477	Two deployments of the passive samplers	463:501	Two deployments of the passive samplers were conducted, during which grab samples were frequently collected for comparison.
25504195	2	44	theme	diethylaminoethyl	333:349	arg1	medium					382:387	a sampling medium	371:387	a sampling medium	371:387	Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana.
25504195	2	44	theme	diethylaminoethyl	333:349	arg1	cellulose					358:366	diethylaminoethyl (DEAE) cellulose	333:366	diethylaminoethyl (DEAE) cellulose	333:366	Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana.
25504195	7	45	theme	DOM	1181:1183	arg1	pool					1185:1188	the bulk DOM pool	1172:1188	the bulk DOM pool	1172:1188	Nonetheless, the passive samplers may provide a cost-effective, integrated sample of DOM in situations where the bulk DOM pool is composed mainly of terrestrial, humic-like compounds.
25504195	7	46	dep	terrestrial	1212:1222	arg1	humic-like					1225:1234	humic-like	1225:1234	humic-like	1225:1234	Nonetheless, the passive samplers may provide a cost-effective, integrated sample of DOM in situations where the bulk DOM pool is composed mainly of terrestrial, humic-like compounds.
25504195	2	47	theme	DOM	249:251	arg1	samplers					253:260	Passive DOM samplers	241:260	Passive DOM samplers	241:260	Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana.
25504195	1	48	theme	dissolved	123:131	arg1	DOM					149:151	DOM	149:151	DOM	149:151	Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints.
25504195	1	48	theme	dissolved	123:131	arg1	matter					141:146	dissolved organic matter	123:146	dissolved organic matter (DOM) in streams	123:163	Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints.
25504195	4	49	theme	DOM	602:604	arg1	quality					606:612	DOM quality	602:612	DOM quality	602:612	Differences in DOM quality between sites and sampling methods were assessed using several common optical analyses.
25504195	7	50	dep	cost-effective	1111:1124	arg1	integrated					1127:1136	integrated	1127:1136	integrated	1127:1136	Nonetheless, the passive samplers may provide a cost-effective, integrated sample of DOM in situations where the bulk DOM pool is composed mainly of terrestrial, humic-like compounds.
25504195	2	51	theme	Passive	241:247	arg1	samplers					253:260	Passive DOM samplers	241:260	Passive DOM samplers	241:260	Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana.
25504195	1	52	theme	organic	133:139	arg1	DOM					149:151	DOM	149:151	DOM	149:151	Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints.
25504195	1	52	theme	organic	133:139	arg1	matter					141:146	dissolved organic matter	123:146	dissolved organic matter (DOM) in streams	123:163	Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints.
25504195	1	53	theme	time	214:217	arg1	constraints					228:238	time and cost constraints	214:238	time and cost constraints	214:238	Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints.
25504195	0	54	theme	organic	63:69	arg1	matter					71:76	dissolved organic matter	53:76	dissolved organic matter in streams	53:87	Evaluation of passive samplers for the collection of dissolved organic matter in streams.
25504195	0	55	from	streams	81:87	arg1	collection					39:48	the collection	35:48	the collection of dissolved organic matter in streams	35:87	Evaluation of passive samplers for the collection of dissolved organic matter in streams.
25504195	4	56	theme	optical	684:690	arg1	analyses					692:699	several common optical analyses	669:699	several common optical analyses	669:699	Differences in DOM quality between sites and sampling methods were assessed using several common optical analyses.
25504195	0	57	theme	dissolved	53:61	arg1	matter					71:76	dissolved organic matter	53:76	dissolved organic matter in streams	53:87	Evaluation of passive samplers for the collection of dissolved organic matter in streams.
25504195	4	58	theme	common	677:682	arg1	analyses					692:699	several common optical analyses	669:699	several common optical analyses	669:699	Differences in DOM quality between sites and sampling methods were assessed using several common optical analyses.
25504195	1	59	theme	cost	223:226	arg1	constraints					228:238	time and cost constraints	214:238	time and cost constraints	214:238	Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints.
28256031	3	0	theme	plasma	985:990	arg1	levels					1004:1009	plasma cholesterol levels	985:1009	plasma cholesterol levels	985:1009	This was performed at 7, 14 and 30 days after start feeding functional diets, and microbiome diversity increased when fish fed functional diets after 7 days and it was positively correlated with plasma cholesterol levels.
28256031	2	1	theme	high-throughput	609:623	arg1	sequencing					625:634	high-throughput sequencing	609:634	high-throughput sequencing	609:634	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	9	2	theme	STUDY	1982:1986	arg1	SIGNIFICANCE					1951:1962	SIGNIFICANCE	1951:1962	SIGNIFICANCE	1951:1962	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	9	2	theme	STUDY	1982:1986	arg1	IMPACT					1968:1973	IMPACT	1968:1973	IMPACT	1968:1973	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	1	3	dep	%	424:424	arg1	cerevisiae					440:449	Saccharomyces cerevisiae	426:449	0·5% Saccharomyces cerevisiae	421:449	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	8	4	theme	Functional	1757:1766	arg1	diets					1768:1772	Functional diets	1757:1772	CONCLUSIONS Functional diets based on pre- or probiotics	1745:1800	CONCLUSIONS Functional diets based on pre- or probiotics dynamically modulate intestinal microbiota of juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance.
28256031	1	5	theme	rainbow	286:292	arg1	trout					294:298	rainbow trout	286:298	rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both	286:486	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	1	5	theme	rainbow	286:292	arg1	mykiss					314:319	Oncorhynchus mykiss	301:319	Oncorhynchus mykiss	301:319	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	2	6	theme	V3-V4	656:660	arg1	region					662:667	the V3-V4 region	652:667	the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity	652:787	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	2	7	theme	microbiome	768:777	arg1	diversity					779:787	microbiome diversity	768:787	microbiome diversity	768:787	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	9	8	theme	first	2013:2017	arg1	time					2019:2022	the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology	2009:2210	time	2019:2022	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	6	9	theme	microbiome	1362:1371	arg1	composition					1373:1383	the microbiome composition	1358:1383	the microbiome composition	1358:1383	A dynamic shift of the microbiome composition was observed with changes after 7 days of feeding and the modulation by functional diets tend to cluster the corresponding groups apart from CTRL group.
28256031	2	10	theme	mucosa	545:550	arg1	contents					556:563	contents	556:563	contents	556:563	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	2	10	theme	mucosa	545:550	arg1	total					511:515	A total	509:515	A total of 57 fish whole intestinal mucosa	509:550	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	0	11	theme	juvenile	142:149	arg1	trout					159:163	juvenile rainbow trout	142:163	juvenile rainbow trout (Oncorhynchus mykiss)	142:185	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	0	11	theme	juvenile	142:149	arg1	mykiss					179:184	Oncorhynchus mykiss	166:184	Oncorhynchus mykiss	166:184	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	9	12	dep	time	2019:2022	arg1	dynamics					2050:2057	the microbiome modulation dynamics	2024:2057	the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae	2024:2128	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	7	13	theme	severe	1665:1670	arg1	reductions					1672:1681	severe reductions	1665:1681	severe reductions on their abundances	1665:1701	The core microbiome showed an overall stability with functional diets, except genus such as Escherichia-Shigella that suffered severe reductions on their abundances when feeding any of the functional diets.
28256031	0	14	from	pre-	64:67	arg1	trout					159:163	juvenile rainbow trout	142:163	juvenile rainbow trout (Oncorhynchus mykiss)	142:185	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	0	14	from	pre-	64:67	arg1	mykiss					179:184	Oncorhynchus mykiss	166:184	Oncorhynchus mykiss	166:184	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	1	15	used	used	204:207	arg2	study					198:202	This study	193:202	This study	193:202	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	9	16	theme	modulation	2039:2048	arg1	dynamics					2050:2057	the microbiome modulation dynamics	2024:2057	the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae	2024:2128	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	6	17	theme	dynamic	1341:1347	arg1	shift					1349:1353	A dynamic shift	1339:1353	A dynamic shift of the microbiome composition	1339:1383	A dynamic shift of the microbiome composition was observed with changes after 7 days of feeding and the modulation by functional diets tend to cluster the corresponding groups apart from CTRL group.
28256031	2	18	theme	rRNA	680:683	arg1	gene					685:688	the 16S rRNA gene	672:688	the 16S rRNA gene	672:688	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	0	19	theme	Oncorhynchus	166:177	arg1	trout					159:163	juvenile rainbow trout	142:163	juvenile rainbow trout (Oncorhynchus mykiss)	142:185	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	0	19	theme	Oncorhynchus	166:177	arg1	mykiss					179:184	Oncorhynchus mykiss	166:184	Oncorhynchus mykiss	166:184	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	8	20	theme	juvenile	1848:1855	arg1	trout					1857:1861	juvenile trout	1848:1861	juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance	1848:1948	CONCLUSIONS Functional diets based on pre- or probiotics dynamically modulate intestinal microbiota of juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance.
28256031	2	21	theme	biochemical	734:744	arg1	indicators					753:762	plasma biochemical health indicators	727:762	plasma biochemical health indicators	727:762	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	8	22	theme	abundance	1884:1892	arg1	shifts					1894:1899	taxonomical abundance shifts	1872:1899	taxonomical abundance shifts that might impact fish physiological performance	1872:1948	CONCLUSIONS Functional diets based on pre- or probiotics dynamically modulate intestinal microbiota of juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance.
28256031	2	23	theme	whole	528:532	arg1	mucosa					545:550	57 fish whole intestinal mucosa	520:550	57 fish whole intestinal mucosa	520:550	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	2	24	theme	health	746:751	arg1	indicators					753:762	plasma biochemical health indicators	727:762	plasma biochemical health indicators	727:762	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	7	25	theme	functional	1591:1600	arg1	diets					1602:1606	functional diets	1591:1606	functional diets	1591:1606	The core microbiome showed an overall stability with functional diets, except genus such as Escherichia-Shigella that suffered severe reductions on their abundances when feeding any of the functional diets.
28256031	2	26	theme	region	662:667	arg1	sequencing					625:634	high-throughput sequencing	609:634	high-throughput sequencing	609:634	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	2	26	theme	region	662:667	arg1	analysis					640:647	analysis	640:647	analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity	640:787	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	0	27	theme	Microbiome	0:9	arg1	modulation					19:28	Microbiome dynamic modulation	0:28	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).	0:186	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	8	28	theme	physiological	1924:1936	arg1	performance					1938:1948	fish physiological performance	1919:1948	fish physiological performance	1919:1948	CONCLUSIONS Functional diets based on pre- or probiotics dynamically modulate intestinal microbiota of juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance.
28256031	1	29	dep	pre-	368:371	arg1	%					391:391	0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae	388:449	%	391:391	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	1	29	dep	pre-	368:371	arg1	%					424:424	0·5%	421:424	0·5% Saccharomyces cerevisiae	421:449	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	8	30	dep	CONCLUSIONS	1745:1755	arg1	diets					1768:1772	Functional diets	1757:1772	CONCLUSIONS Functional diets based on pre- or probiotics	1745:1800	CONCLUSIONS Functional diets based on pre- or probiotics dynamically modulate intestinal microbiota of juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance.
28256031	6	31	theme	functional	1457:1466	arg1	diets					1468:1472	functional diets	1457:1472	functional diets	1457:1472	A dynamic shift of the microbiome composition was observed with changes after 7 days of feeding and the modulation by functional diets tend to cluster the corresponding groups apart from CTRL group.
28256031	1	32	theme	Oncorhynchus	301:312	arg1	trout					294:298	rainbow trout	286:298	rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both	286:486	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	1	32	theme	Oncorhynchus	301:312	arg1	mykiss					314:319	Oncorhynchus mykiss	301:319	Oncorhynchus mykiss	301:319	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	7	33	theme	overall	1568:1574	arg1	stability					1576:1584	an overall stability	1565:1584	an overall stability with functional diets	1565:1606	The core microbiome showed an overall stability with functional diets, except genus such as Escherichia-Shigella that suffered severe reductions on their abundances when feeding any of the functional diets.
28256031	0	34	dep	pre-	64:67	arg1	mannan-oligosaccharides					85:107	mannan-oligosaccharides	85:107	mannan-oligosaccharides	85:107	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	0	34	dep	pre-	64:67	arg1	cerevisiae					127:136	Saccharomyces cerevisiae	113:136	Saccharomyces cerevisiae	113:136	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	4	35	theme	Dominant	1012:1019	arg1	phyla					1021:1025	Dominant phyla	1012:1025	Dominant phyla	1012:1025	Dominant phyla were, in descending order, Proteobacteria, Firmicutes, Actinobacteria, Acidobacteria, Bacteroidetes and Fusobacteria.
28256031	9	36	theme	independent	2162:2172	arg1	technology					2201:2210	culture independent high-throughput sequencing technology	2154:2210	culture independent high-throughput sequencing technology	2154:2210	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	1	37	dep	AIMS	188:191	arg1	used					204:207	used	204:207	used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both	204:486	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	0	38	theme	functional	38:47	arg1	diets					49:53	functional diets	38:53	functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss)	38:185	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	9	39	theme	sequencing	2190:2199	arg1	technology					2201:2210	culture independent high-throughput sequencing technology	2154:2210	culture independent high-throughput sequencing technology	2154:2210	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	1	40	theme	commercial	326:335	arg1	diets					337:341	commercial diets	326:341	commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both	326:486	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	7	41	theme	core	1542:1545	arg1	microbiome					1547:1556	The core microbiome	1538:1556	The core microbiome	1538:1556	The core microbiome showed an overall stability with functional diets, except genus such as Escherichia-Shigella that suffered severe reductions on their abundances when feeding any of the functional diets.
28256031	6	42	theme	CTRL	1526:1529	arg1	group					1531:1535	CTRL group	1526:1535	CTRL group	1526:1535	A dynamic shift of the microbiome composition was observed with changes after 7 days of feeding and the modulation by functional diets tend to cluster the corresponding groups apart from CTRL group.
28256031	3	43	theme	cholesterol	992:1002	arg1	levels					1004:1009	plasma cholesterol levels	985:1009	plasma cholesterol levels	985:1009	This was performed at 7, 14 and 30 days after start feeding functional diets, and microbiome diversity increased when fish fed functional diets after 7 days and it was positively correlated with plasma cholesterol levels.
28256031	6	44	theme	corresponding	1494:1506	arg1	groups					1508:1513	the corresponding groups	1490:1513	the corresponding groups apart from CTRL group	1490:1535	A dynamic shift of the microbiome composition was observed with changes after 7 days of feeding and the modulation by functional diets tend to cluster the corresponding groups apart from CTRL group.
28256031	1	45	theme	microbiome	263:272	arg1	dynamics					274:281	the intestinal microbiome dynamics	248:281	the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both	248:486	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	9	46	theme	dietary	2249:2255	arg1	modulation					2257:2266	the dietary modulation	2245:2266	the dietary modulation	2245:2266	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	5	47	theme	organisms	1240:1248	arg1	abundances					1226:1235	abundances	1226:1235	abundances of organisms from Firmicutes and Fusobacteria, two phyla	1226:1292	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	0	48	from	trout	159:163	arg1	pre-					64:67	pre-	64:67	pre-	64:67	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	1	49	theme	both	483:486	arg1	mixture					472:478	the mixture	468:478	the mixture of both	468:486	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	9	50	theme	aquatic	2293:2299	arg1	organisms					2301:2309	aquatic organisms	2293:2309	aquatic organisms	2293:2309	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	6	51	theme	composition	1373:1383	arg1	shift					1349:1353	A dynamic shift	1339:1353	A dynamic shift of the microbiome composition	1339:1383	A dynamic shift of the microbiome composition was observed with changes after 7 days of feeding and the modulation by functional diets tend to cluster the corresponding groups apart from CTRL group.
28256031	9	52	dep	SIGNIFICANCE	1951:1962	arg1	shows					1999:2003	shows	1999:2003	shows	1999:2003	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	0	53	theme	rainbow	151:157	arg1	trout					159:163	juvenile rainbow trout	142:163	juvenile rainbow trout (Oncorhynchus mykiss)	142:185	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	0	53	theme	rainbow	151:157	arg1	mykiss					179:184	Oncorhynchus mykiss	166:184	Oncorhynchus mykiss	166:184	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	7	54	with	stability	1576:1584	arg1	diets					1602:1606	functional diets	1591:1606	functional diets	1591:1606	The core microbiome showed an overall stability with functional diets, except genus such as Escherichia-Shigella that suffered severe reductions on their abundances when feeding any of the functional diets.
28256031	1	55	theme	high-throughput	209:223	arg1	sequencing					225:234	high-throughput sequencing	209:234	high-throughput sequencing	209:234	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	2	56	theme	intestinal	534:543	arg1	mucosa					545:550	57 fish whole intestinal mucosa	520:550	57 fish whole intestinal mucosa	520:550	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	2	57	theme	relationship	706:717	arg1	region					662:667	the V3-V4 region	652:667	the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity	652:787	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	5	58	from	Fusobacteria	1270:1281	arg1	organisms					1240:1248	organisms	1240:1248	organisms from Firmicutes and Fusobacteria	1240:1281	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	5	58	from	Fusobacteria	1270:1281	arg1	abundances					1226:1235	abundances	1226:1235	abundances of organisms from Firmicutes and Fusobacteria, two phyla	1226:1292	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	5	58	from	Fusobacteria	1270:1281	arg1	phyla					1288:1292	two phyla	1284:1292	two phyla	1284:1292	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	5	59	from	Firmicutes	1255:1264	arg1	organisms					1240:1248	organisms	1240:1248	organisms from Firmicutes and Fusobacteria	1240:1281	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	5	59	from	Firmicutes	1255:1264	arg1	abundances					1226:1235	abundances	1226:1235	abundances of organisms from Firmicutes and Fusobacteria, two phyla	1226:1292	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	5	59	from	Firmicutes	1255:1264	arg1	phyla					1288:1292	two phyla	1284:1292	two phyla	1284:1292	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	8	60	theme	intestinal	1823:1832	arg1	microbiota					1834:1843	intestinal microbiota	1823:1843	intestinal microbiota of juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance	1823:1948	CONCLUSIONS Functional diets based on pre- or probiotics dynamically modulate intestinal microbiota of juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance.
28256031	2	61	theme	bacterial	565:573	arg1	communities					575:585	bacterial communities	565:585	bacterial communities	565:585	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	9	62	theme	functional	2062:2071	arg1	diets					2073:2077	functional diets	2062:2077	functional diets based on mannan-oligosaccharides and S. cerevisiae	2062:2128	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	5	63	theme	beneficial	1319:1328	arg1	effects					1330:1336	beneficial effects	1319:1336	beneficial effects	1319:1336	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	6	64	theme	feeding	1427:1433	arg1	7 days					1417:1422	7 days	1417:1422	7 days of feeding	1417:1433	A dynamic shift of the microbiome composition was observed with changes after 7 days of feeding and the modulation by functional diets tend to cluster the corresponding groups apart from CTRL group.
28256031	3	65	theme	functional	850:859	arg1	diets					861:865	functional diets	850:865	functional diets	850:865	This was performed at 7, 14 and 30 days after start feeding functional diets, and microbiome diversity increased when fish fed functional diets after 7 days and it was positively correlated with plasma cholesterol levels.
28256031	2	66	theme	plasma	727:732	arg1	indicators					753:762	plasma biochemical health indicators	727:762	plasma biochemical health indicators	727:762	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	8	67	theme	trout	1857:1861	arg1	microbiota					1834:1843	intestinal microbiota	1823:1843	intestinal microbiota of juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance	1823:1948	CONCLUSIONS Functional diets based on pre- or probiotics dynamically modulate intestinal microbiota of juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance.
28256031	2	68	theme	16S	676:678	arg1	gene					685:688	the 16S rRNA gene	672:688	the 16S rRNA gene	672:688	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	5	69	theme	functional	1154:1163	arg1	diets					1165:1169	functional diets	1154:1169	functional diets	1154:1169	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	2	70	dep	METHODS	489:495	arg1	characterized					592:604	characterized	592:604	were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity	587:787	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	8	71	theme	taxonomical	1872:1882	arg1	shifts					1894:1899	taxonomical abundance shifts	1872:1899	taxonomical abundance shifts that might impact fish physiological performance	1872:1948	CONCLUSIONS Functional diets based on pre- or probiotics dynamically modulate intestinal microbiota of juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance.
28256031	3	72	theme	microbiome	872:881	arg1	diversity					883:891	microbiome diversity	872:891	microbiome diversity	872:891	This was performed at 7, 14 and 30 days after start feeding functional diets, and microbiome diversity increased when fish fed functional diets after 7 days and it was positively correlated with plasma cholesterol levels.
28256031	2	73	theme	fish	523:526	arg1	mucosa					545:550	57 fish whole intestinal mucosa	520:550	57 fish whole intestinal mucosa	520:550	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	7	74	from	reductions	1672:1681	arg1	abundances					1692:1701	their abundances	1686:1701	their abundances	1686:1701	The core microbiome showed an overall stability with functional diets, except genus such as Escherichia-Shigella that suffered severe reductions on their abundances when feeding any of the functional diets.
28256031	3	75	theme	functional	917:926	arg1	diets					928:932	functional diets	917:932	functional diets	917:932	This was performed at 7, 14 and 30 days after start feeding functional diets, and microbiome diversity increased when fish fed functional diets after 7 days and it was positively correlated with plasma cholesterol levels.
28256031	0	76	theme	dynamic	11:17	arg1	modulation					19:28	Microbiome dynamic modulation	0:28	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).	0:186	Microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and Saccharomyces cerevisiae) in juvenile rainbow trout (Oncorhynchus mykiss).
28256031	7	77	theme	functional	1727:1736	arg1	diets					1738:1742	the functional diets	1723:1742	the functional diets	1723:1742	The core microbiome showed an overall stability with functional diets, except genus such as Escherichia-Shigella that suffered severe reductions on their abundances when feeding any of the functional diets.
28256031	5	78	from	abundances	1226:1235	arg1	Fusobacteria					1270:1281	Fusobacteria	1270:1281	Fusobacteria	1270:1281	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	5	78	from	abundances	1226:1235	arg1	Firmicutes					1255:1264	Firmicutes	1255:1264	Firmicutes	1255:1264	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	2	79	theme	gene	685:688	arg1	region					662:667	the V3-V4 region	652:667	the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity	652:787	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	5	80	theme	Gammaproteobacteria	1196:1214	arg1	abundance					1183:1191	the abundance	1179:1191	the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects	1179:1336	However, functional diets reduced the abundance of Gammaproteobacteria to favour abundances of organisms from Firmicutes and Fusobacteria, two phyla with members that confer beneficial effects.
28256031	8	81	theme	fish	1919:1922	arg1	performance					1938:1948	fish physiological performance	1919:1948	fish physiological performance	1919:1948	CONCLUSIONS Functional diets based on pre- or probiotics dynamically modulate intestinal microbiota of juvenile trout engaging taxonomical abundance shifts that might impact fish physiological performance.
28256031	9	82	theme	culture	2154:2160	arg1	technology					2201:2210	culture independent high-throughput sequencing technology	2154:2210	culture independent high-throughput sequencing technology	2154:2210	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	9	83	theme	high-throughput	2174:2188	arg1	technology					2201:2210	culture independent high-throughput sequencing technology	2154:2210	culture independent high-throughput sequencing technology	2154:2210	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	2	84	dep	RESULTS	501:507	arg1	contents					556:563	contents	556:563	contents	556:563	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	2	84	dep	RESULTS	501:507	arg1	total					511:515	A total	509:515	A total of 57 fish whole intestinal mucosa	509:550	METHODS AND RESULTS A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity.
28256031	1	85	from	dynamics	274:281	arg1	trout					294:298	rainbow trout	286:298	rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both	286:486	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	1	85	from	dynamics	274:281	arg1	mykiss					314:319	Oncorhynchus mykiss	301:319	Oncorhynchus mykiss	301:319	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	9	86	theme	microbiome	2028:2037	arg1	dynamics					2050:2057	the microbiome modulation dynamics	2024:2057	the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae	2024:2128	SIGNIFICANCE AND IMPACT OF THE STUDY This study shows for the first time the microbiome modulation dynamics by functional diets based on mannan-oligosaccharides and S. cerevisiae and their synergy using culture independent high-throughput sequencing technology, revealing the complexity behind the dietary modulation with functional feeds in aquatic organisms.
28256031	1	87	dep	%	391:391	arg1	mannan-oligosaccharides					393:415	mannan-oligosaccharides	393:415	mannan-oligosaccharides	393:415	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
28256031	1	88	theme	intestinal	252:261	arg1	dynamics					274:281	the intestinal microbiome dynamics	248:281	the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both	248:486	AIMS This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% Saccharomyces cerevisiae respectively) or the mixture of both.
25820256	9	0	from	adiposity	1114:1122	arg1	phenotypes					1158:1167	both prone and resistant obese phenotypes	1127:1167	both prone and resistant obese phenotypes	1127:1167	CONCLUSIONS OFS was able to reduce body weight and adiposity in both prone and resistant obese phenotypes.
25820256	6	1	theme	body	733:736	arg1	weight					738:743	body weight	733:743	body weight	733:743	RESULTS OFS reduced body weight, energy intake, and fat mass in both phenotypes (P < 0.05).
25820256	1	2	theme	satiety	227:233	arg1	hormones					235:242	gut satiety hormones	223:242	gut satiety hormones	223:242	OBJECTIVE Oligofructose (OFS) is a prebiotic that reduces energy intake and fat mass via changes in gut satiety hormones and microbiota.
25820256	5	3	theme	cannabinoid	638:648	arg1	expression					665:674	cannabinoid CB(1) receptor expression	638:674	cannabinoid CB(1) receptor expression	638:674	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	0	4	from	effects	12:18	arg1	microbiota					84:93	microbiota	84:93	microbiota in diet-induced obese rats	84:120	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats.
25820256	0	4	from	effects	12:18	arg1	hormones					71:78	gut hormones	67:78	gut hormones	67:78	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats.
25820256	10	5	theme	gut	1193:1195	arg1	profiles					1208:1215	gut microbiota profiles	1193:1215	gut microbiota profiles in DIO and DR rats	1193:1234	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	10	6	from	changes	1248:1254	arg1	levels					1271:1276	gut hormone levels	1259:1276	gut hormone levels	1259:1276	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	6	7	theme	RESULTS	713:719	arg1	OFS					721:723	RESULTS OFS	713:723	RESULTS OFS	713:723	RESULTS OFS reduced body weight, energy intake, and fat mass in both phenotypes (P < 0.05).
25820256	10	8	theme	microbiota	1197:1206	arg1	profiles					1208:1215	gut microbiota profiles	1193:1215	gut microbiota profiles in DIO and DR rats	1193:1234	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	0	9	theme	diet-induced	98:109	arg1	rats					117:120	diet-induced obese rats	98:120	diet-induced obese rats	98:120	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats.
25820256	9	10	from	weight	1103:1108	arg1	phenotypes					1158:1167	both prone and resistant obese phenotypes	1127:1167	both prone and resistant obese phenotypes	1127:1167	CONCLUSIONS OFS was able to reduce body weight and adiposity in both prone and resistant obese phenotypes.
25820256	10	11	theme	sustained	1304:1312	arg1	weights					1325:1331	the sustained lower body weights	1300:1331	the sustained lower body weights	1300:1331	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	7	12	theme	gut	812:814	arg1	microbiota					816:825	Select gut microbiota	805:825	Select gut microbiota	805:825	Select gut microbiota differed in DIO versus DR rats (P < 0.05), the differences being eliminated by OFS.
25820256	4	13	theme	high-fat/high-sucrose	498:518	arg1	diet					526:529	high-fat/high-sucrose (HFS) diet	498:529	high-fat/high-sucrose (HFS) diet	498:529	METHODS Adult, male DIO, and DR rats were randomized to: high-fat/high-sucrose (HFS) diet or HFS diet + 10% OFS for 6 weeks.
25820256	6	14	theme	energy	746:751	arg1	intake					753:758	energy intake	746:758	energy intake	746:758	RESULTS OFS reduced body weight, energy intake, and fat mass in both phenotypes (P < 0.05).
25820256	8	15	dep	elevated	1035:1042	arg1	P < 0.05					1045:1052	P < 0.05	1045:1052	P < 0.05	1045:1052	OFS did not modify plasma ghrelin or CB(1) expression in nodose ganglia, but plasma levels of GIP were reduced and PYY were elevated (P < 0.05) by OFS.
25820256	5	16	from	intake	589:594	arg1	ganglia					690:696	the nodose ganglia	679:696	the nodose ganglia	679:696	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	5	17	theme	plasma	613:618	arg1	hormones					624:631	plasma gut hormones	613:631	plasma gut hormones	613:631	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	9	18	theme	CONCLUSIONS	1063:1073	arg1	OFS					1075:1077	CONCLUSIONS OFS	1063:1077	CONCLUSIONS OFS	1063:1077	CONCLUSIONS OFS was able to reduce body weight and adiposity in both prone and resistant obese phenotypes.
25820256	3	19	theme	diet-induced	386:397	arg1	DIO					406:408	diet-induced obese (DIO) and diet-resistant (DR) rats	386:438	DIO	406:408	The aim of this study was to examine the effect of OFS in diet-induced obese (DIO) and diet-resistant (DR) rats.
25820256	0	20	theme	obese	111:115	arg1	rats					117:120	diet-induced obese rats	98:120	diet-induced obese rats	98:120	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats.
25820256	5	21	theme	CB	650:651	arg1	expression					665:674	cannabinoid CB(1) receptor expression	638:674	cannabinoid CB(1) receptor expression	638:674	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	8	22	from	ghrelin	937:943	arg1	ganglia					975:981	nodose ganglia	968:981	nodose ganglia	968:981	OFS did not modify plasma ghrelin or CB(1) expression in nodose ganglia, but plasma levels of GIP were reduced and PYY were elevated (P < 0.05) by OFS.
25820256	10	23	dep	along	1237:1241	arg1	with					1243:1246	with	1243:1246	with	1243:1246	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	5	24	from	microbiota	601:610	arg1	ganglia					690:696	the nodose ganglia	679:696	the nodose ganglia	679:696	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	3	25	theme	obese	399:403	arg1	DIO					406:408	diet-induced obese (DIO) and diet-resistant (DR) rats	386:438	DIO	406:408	The aim of this study was to examine the effect of OFS in diet-induced obese (DIO) and diet-resistant (DR) rats.
25820256	5	26	theme	receptor	656:663	arg1	expression					665:674	cannabinoid CB(1) receptor expression	638:674	cannabinoid CB(1) receptor expression	638:674	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	5	27	from	expression	665:674	arg1	ganglia					690:696	the nodose ganglia	679:696	the nodose ganglia	679:696	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	10	28	from	profiles	1208:1215	arg1	rats					1231:1234	DIO and DR rats	1220:1234	rats	1231:1234	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	3	29	theme	OFS	379:381	arg1	effect					369:374	the effect	365:374	the effect of OFS in diet-induced obese (DIO) and diet-resistant (DR) rats	365:438	The aim of this study was to examine the effect of OFS in diet-induced obese (DIO) and diet-resistant (DR) rats.
25820256	0	30	theme	Interactive	0:10	arg1	effects					12:18	Interactive effects	0:18	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats	0:120	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats.
25820256	4	31	theme	DR	470:471	arg1	rats					473:476	DR rats	470:476	DR rats	470:476	METHODS Adult, male DIO, and DR rats were randomized to: high-fat/high-sucrose (HFS) diet or HFS diet + 10% OFS for 6 weeks.
25820256	8	32	from	expression	954:963	arg1	ganglia					975:981	nodose ganglia	968:981	nodose ganglia	968:981	OFS did not modify plasma ghrelin or CB(1) expression in nodose ganglia, but plasma levels of GIP were reduced and PYY were elevated (P < 0.05) by OFS.
25820256	1	33	theme	energy	181:186	arg1	intake					188:193	energy intake	181:193	energy intake	181:193	OBJECTIVE Oligofructose (OFS) is a prebiotic that reduces energy intake and fat mass via changes in gut satiety hormones and microbiota.
25820256	0	34	theme	oligofructose	23:35	arg1	effects					12:18	Interactive effects	0:18	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats	0:120	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats.
25820256	10	35	from	changes	1182:1188	arg1	profiles					1208:1215	gut microbiota profiles	1193:1215	gut microbiota profiles in DIO and DR rats	1193:1234	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	5	36	theme	food	584:587	arg1	intake					589:594	food intake	584:594	food intake	584:594	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	8	37	theme	plasma	988:993	arg1	levels					995:1000	plasma levels	988:1000	plasma levels of GIP	988:1007	OFS did not modify plasma ghrelin or CB(1) expression in nodose ganglia, but plasma levels of GIP were reduced and PYY were elevated (P < 0.05) by OFS.
25820256	4	38	theme	HFS	534:536	arg1	%					547:547	HFS diet + 10%	534:547	HFS diet + 10%	534:547	METHODS Adult, male DIO, and DR rats were randomized to: high-fat/high-sucrose (HFS) diet or HFS diet + 10% OFS for 6 weeks.
25820256	3	39	theme	study	344:348	arg1	aim					332:334	The aim	328:334	The aim of this study	328:348	The aim of this study was to examine the effect of OFS in diet-induced obese (DIO) and diet-resistant (DR) rats.
25820256	0	40	from	microbiota	84:93	arg1	rats					117:120	diet-induced obese rats	98:120	diet-induced obese rats	98:120	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats.
25820256	2	41	theme	OFS	275:277	arg1	effects					264:270	The effects	260:270	The effects of OFS	260:277	The effects of OFS may vary depending on predisposition to obesity.
25820256	8	42	theme	1	951:951	arg1	expression					954:963	CB(1) expression	948:963	CB(1) expression	948:963	OFS did not modify plasma ghrelin or CB(1) expression in nodose ganglia, but plasma levels of GIP were reduced and PYY were elevated (P < 0.05) by OFS.
25820256	0	43	theme	obesity	41:47	arg1	effects					12:18	Interactive effects	0:18	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats	0:120	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats.
25820256	9	44	theme	body	1098:1101	arg1	weight					1103:1108	body weight	1098:1108	body weight	1098:1108	CONCLUSIONS OFS was able to reduce body weight and adiposity in both prone and resistant obese phenotypes.
25820256	10	45	theme	lower	1314:1318	arg1	weights					1325:1331	the sustained lower body weights	1300:1331	the sustained lower body weights	1300:1331	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	7	46	dep	differed	827:834	arg1	eliminated					892:901	eliminated	892:901	being eliminated by OFS	886:908	Select gut microbiota differed in DIO versus DR rats (P < 0.05), the differences being eliminated by OFS.
25820256	1	47	theme	fat	199:201	arg1	mass					203:206	fat mass	199:206	fat mass	199:206	OBJECTIVE Oligofructose (OFS) is a prebiotic that reduces energy intake and fat mass via changes in gut satiety hormones and microbiota.
25820256	4	48	theme	male	456:459	arg1	DIO					461:463	male DIO	456:463	male DIO	456:463	METHODS Adult, male DIO, and DR rats were randomized to: high-fat/high-sucrose (HFS) diet or HFS diet + 10% OFS for 6 weeks.
25820256	4	48	theme	male	456:459	arg1	METHODS					441:447	METHODS	441:447	METHODS Adult, male DIO, and DR rats	441:476	METHODS Adult, male DIO, and DR rats were randomized to: high-fat/high-sucrose (HFS) diet or HFS diet + 10% OFS for 6 weeks.
25820256	5	49	theme	gut	597:599	arg1	microbiota					601:610	gut microbiota	597:610	gut microbiota	597:610	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	0	50	from	hormones	71:78	arg1	rats					117:120	diet-induced obese rats	98:120	diet-induced obese rats	98:120	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats.
25820256	10	51	theme	body	1320:1323	arg1	weights					1325:1331	the sustained lower body weights	1300:1331	the sustained lower body weights	1300:1331	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	9	52	theme	obese	1152:1156	arg1	phenotypes					1158:1167	both prone and resistant obese phenotypes	1127:1167	both prone and resistant obese phenotypes	1127:1167	CONCLUSIONS OFS was able to reduce body weight and adiposity in both prone and resistant obese phenotypes.
25820256	5	53	theme	gut	620:622	arg1	hormones					624:631	plasma gut hormones	613:631	plasma gut hormones	613:631	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	4	54	dep	METHODS	441:447	arg1	DIO					461:463	male DIO	456:463	male DIO	456:463	METHODS Adult, male DIO, and DR rats were randomized to: high-fat/high-sucrose (HFS) diet or HFS diet + 10% OFS for 6 weeks.
25820256	4	54	dep	METHODS	441:447	arg1	METHODS					441:447	METHODS	441:447	METHODS Adult, male DIO, and DR rats	441:476	METHODS Adult, male DIO, and DR rats were randomized to: high-fat/high-sucrose (HFS) diet or HFS diet + 10% OFS for 6 weeks.
25820256	4	54	dep	METHODS	441:447	arg1	rats					473:476	DR rats	470:476	DR rats	470:476	METHODS Adult, male DIO, and DR rats were randomized to: high-fat/high-sucrose (HFS) diet or HFS diet + 10% OFS for 6 weeks.
25820256	4	54	dep	METHODS	441:447	arg1	Adult					449:453	Adult	449:453	Adult	449:453	METHODS Adult, male DIO, and DR rats were randomized to: high-fat/high-sucrose (HFS) diet or HFS diet + 10% OFS for 6 weeks.
25820256	10	55	theme	gut	1259:1261	arg1	levels					1271:1276	gut hormone levels	1259:1276	gut hormone levels	1259:1276	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	8	56	theme	plasma	930:935	arg1	ghrelin					937:943	plasma ghrelin	930:943	plasma ghrelin	930:943	OFS did not modify plasma ghrelin or CB(1) expression in nodose ganglia, but plasma levels of GIP were reduced and PYY were elevated (P < 0.05) by OFS.
25820256	7	57	theme	DR	850:851	arg1	P < 0.05					859:866	P < 0.05	859:866	P < 0.05	859:866	Select gut microbiota differed in DIO versus DR rats (P < 0.05), the differences being eliminated by OFS.
25820256	7	57	theme	DR	850:851	arg1	rats					853:856	DIO versus DR rats	839:856	rats	853:856	Select gut microbiota differed in DIO versus DR rats (P < 0.05), the differences being eliminated by OFS.
25820256	3	58	theme	DR	431:432	arg1	rats					435:438	diet-induced obese (DIO) and diet-resistant (DR) rats	386:438	rats	435:438	The aim of this study was to examine the effect of OFS in diet-induced obese (DIO) and diet-resistant (DR) rats.
25820256	10	59	theme	DR	1228:1229	arg1	rats					1231:1234	DIO and DR rats	1220:1234	rats	1231:1234	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	5	60	theme	Body	566:569	arg1	composition					571:581	Body composition	566:581	Body composition	566:581	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	4	61	dep	diet	526:529	arg1	OFS					549:551	OFS	549:551	OFS	549:551	METHODS Adult, male DIO, and DR rats were randomized to: high-fat/high-sucrose (HFS) diet or HFS diet + 10% OFS for 6 weeks.
25820256	10	62	theme	DIO	1220:1222	arg1	rats					1231:1234	DIO and DR rats	1220:1234	rats	1231:1234	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	1	63	theme	OBJECTIVE	123:131	arg1	Oligofructose					133:145	OBJECTIVE Oligofructose	123:145	OBJECTIVE Oligofructose (OFS)	123:151	OBJECTIVE Oligofructose (OFS) is a prebiotic that reduces energy intake and fat mass via changes in gut satiety hormones and microbiota.
25820256	1	63	theme	OBJECTIVE	123:131	arg1	prebiotic					158:166	a prebiotic	156:166	a prebiotic that reduces energy intake and fat mass via changes in gut satiety hormones and microbiota	156:257	OBJECTIVE Oligofructose (OFS) is a prebiotic that reduces energy intake and fat mass via changes in gut satiety hormones and microbiota.
25820256	1	63	theme	OBJECTIVE	123:131	arg1	OFS					148:150	OFS	148:150	OFS	148:150	OBJECTIVE Oligofructose (OFS) is a prebiotic that reduces energy intake and fat mass via changes in gut satiety hormones and microbiota.
25820256	8	64	theme	nodose	968:973	arg1	ganglia					975:981	nodose ganglia	968:981	nodose ganglia	968:981	OFS did not modify plasma ghrelin or CB(1) expression in nodose ganglia, but plasma levels of GIP were reduced and PYY were elevated (P < 0.05) by OFS.
25820256	10	65	theme	OFS-induced	1170:1180	arg1	changes					1182:1188	OFS-induced changes	1170:1188	OFS-induced changes in gut microbiota profiles in DIO and DR rats	1170:1234	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	0	66	dep	oligofructose	23:35	arg1	predisposition					49:62	predisposition	49:62	predisposition	49:62	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats.
25820256	7	67	theme	DIO	839:841	arg1	P < 0.05					859:866	P < 0.05	859:866	P < 0.05	859:866	Select gut microbiota differed in DIO versus DR rats (P < 0.05), the differences being eliminated by OFS.
25820256	7	67	theme	DIO	839:841	arg1	rats					853:856	DIO versus DR rats	839:856	rats	853:856	Select gut microbiota differed in DIO versus DR rats (P < 0.05), the differences being eliminated by OFS.
25820256	3	68	theme	diet-resistant	415:428	arg1	rats					435:438	diet-induced obese (DIO) and diet-resistant (DR) rats	386:438	rats	435:438	The aim of this study was to examine the effect of OFS in diet-induced obese (DIO) and diet-resistant (DR) rats.
25820256	5	69	from	composition	571:581	arg1	ganglia					690:696	the nodose ganglia	679:696	the nodose ganglia	679:696	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	3	70	from	effect	369:374	arg1	DIO					406:408	diet-induced obese (DIO) and diet-resistant (DR) rats	386:438	DIO	406:408	The aim of this study was to examine the effect of OFS in diet-induced obese (DIO) and diet-resistant (DR) rats.
25820256	3	70	from	effect	369:374	arg1	rats					435:438	diet-induced obese (DIO) and diet-resistant (DR) rats	386:438	rats	435:438	The aim of this study was to examine the effect of OFS in diet-induced obese (DIO) and diet-resistant (DR) rats.
25820256	1	71	from	changes	212:218	arg1	hormones					235:242	gut satiety hormones	223:242	gut satiety hormones	223:242	OBJECTIVE Oligofructose (OFS) is a prebiotic that reduces energy intake and fat mass via changes in gut satiety hormones and microbiota.
25820256	1	71	from	changes	212:218	arg1	microbiota					248:257	microbiota	248:257	microbiota	248:257	OBJECTIVE Oligofructose (OFS) is a prebiotic that reduces energy intake and fat mass via changes in gut satiety hormones and microbiota.
25820256	5	72	from	hormones	624:631	arg1	ganglia					690:696	the nodose ganglia	679:696	the nodose ganglia	679:696	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	9	73	theme	prone	1132:1136	arg1	phenotypes					1158:1167	both prone and resistant obese phenotypes	1127:1167	both prone and resistant obese phenotypes	1127:1167	CONCLUSIONS OFS was able to reduce body weight and adiposity in both prone and resistant obese phenotypes.
25820256	5	74	theme	nodose	683:688	arg1	ganglia					690:696	the nodose ganglia	679:696	the nodose ganglia	679:696	Body composition, food intake, gut microbiota, plasma gut hormones, and cannabinoid CB(1) receptor expression in the nodose ganglia were measured.
25820256	8	75	theme	GIP	1005:1007	arg1	levels					995:1000	plasma levels	988:1000	plasma levels of GIP	988:1007	OFS did not modify plasma ghrelin or CB(1) expression in nodose ganglia, but plasma levels of GIP were reduced and PYY were elevated (P < 0.05) by OFS.
25820256	10	76	theme	hormone	1263:1269	arg1	levels					1271:1276	gut hormone levels	1259:1276	gut hormone levels	1259:1276	OFS-induced changes in gut microbiota profiles in DIO and DR rats, along with changes in gut hormone levels, likely contribute to the sustained lower body weights.
25820256	0	77	theme	gut	67:69	arg1	hormones					71:78	gut hormones	67:78	gut hormones	67:78	Interactive effects of oligofructose and obesity predisposition on gut hormones and microbiota in diet-induced obese rats.
25820256	6	78	theme	fat	765:767	arg1	mass					769:772	fat mass	765:772	fat mass	765:772	RESULTS OFS reduced body weight, energy intake, and fat mass in both phenotypes (P < 0.05).
25820256	9	79	theme	resistant	1142:1150	arg1	phenotypes					1158:1167	both prone and resistant obese phenotypes	1127:1167	both prone and resistant obese phenotypes	1127:1167	CONCLUSIONS OFS was able to reduce body weight and adiposity in both prone and resistant obese phenotypes.
25820256	7	80	theme	Select	805:810	arg1	microbiota					816:825	Select gut microbiota	805:825	Select gut microbiota	805:825	Select gut microbiota differed in DIO versus DR rats (P < 0.05), the differences being eliminated by OFS.
25820256	4	81	theme	HFS	521:523	arg1	diet					526:529	high-fat/high-sucrose (HFS) diet	498:529	high-fat/high-sucrose (HFS) diet	498:529	METHODS Adult, male DIO, and DR rats were randomized to: high-fat/high-sucrose (HFS) diet or HFS diet + 10% OFS for 6 weeks.
25820256	1	82	theme	gut	223:225	arg1	hormones					235:242	gut satiety hormones	223:242	gut satiety hormones	223:242	OBJECTIVE Oligofructose (OFS) is a prebiotic that reduces energy intake and fat mass via changes in gut satiety hormones and microbiota.
26753645	4	0	theme	hydroxyethyl	533:544	arg1	starch					546:551	hydroxyethyl starch	533:551	hydroxyethyl starch 40 injection	533:564	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	7	1	theme	lacic	952:956	arg1	LA					964:965	LA	964:965	LA	964:965	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	1	theme	lacic	952:956	arg1	acid					958:961	arterial blood lacic acid	937:961	arterial blood lacic acid (LA)	937:966	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	4	2	theme	40	553:554	arg1	injection					556:564	injection	556:564	injection	556:564	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	7	3	theme	arterial	937:944	arg1	LA					964:965	LA	964:965	LA	964:965	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	3	theme	arterial	937:944	arg1	acid					958:961	arterial blood lacic acid	937:961	arterial blood lacic acid (LA)	937:966	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	13	4	theme	T₂and	1813:1817	arg1	resuscitation					1827:1839	T₂and T₃after resuscitation	1813:1839	T₂and T₃after resuscitation	1813:1839	At T₂and T₃after resuscitation, CRP in both Group A and Group B was significantly increased compared with that upon admission to hospital, and was lower in Group B than in Group A.
26753645	2	5	from	effect	200:205	arg1	resuscitation					270:282	the emergency resuscitation	256:282	the emergency resuscitation for patients with traumatic shock	256:316	The aim of this study is to investigate the effect of different resuscitation fluid compositions on the emergency resuscitation for patients with traumatic shock.
26753645	9	6	theme	average	1220:1226	arg1	pressure					1237:1244	the average arterial pressure	1216:1244	the average arterial pressure in Group B	1216:1255	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	6	theme	average	1220:1226	arg1	higher					1275:1280	higher	1275:1280	higher	1275:1280	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	7	7	theme	prothrombin	914:924	arg1	PT					932:933	PT	932:933	PT	932:933	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	7	theme	prothrombin	914:924	arg1	time					926:929	prothrombin time	914:929	prothrombin time (PT)	914:934	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	2	8	theme	traumatic	302:310	arg1	shock					312:316	traumatic shock	302:316	traumatic shock	302:316	The aim of this study is to investigate the effect of different resuscitation fluid compositions on the emergency resuscitation for patients with traumatic shock.
26753645	2	9	theme	resuscitation	220:232	arg1	compositions					240:251	different resuscitation fluid compositions	210:251	different resuscitation fluid compositions	210:251	The aim of this study is to investigate the effect of different resuscitation fluid compositions on the emergency resuscitation for patients with traumatic shock.
26753645	9	10	theme	Group	1249:1253	arg1	B					1255:1255	Group B	1249:1255	Group B	1249:1255	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	8	11	theme	Tissue	1026:1031	arg1	oxygenation					1033:1043	Tissue oxygenation	1026:1043	Tissue oxygenation	1026:1043	Tissue oxygenation and hemodynamic profile were also analyzed.
26753645	13	12	theme	Group	1866:1870	arg1	B					1872:1872	Group B	1866:1872	Group B	1866:1872	At T₂and T₃after resuscitation, CRP in both Group A and Group B was significantly increased compared with that upon admission to hospital, and was lower in Group B than in Group A.
26753645	12	13	theme	significant	1724:1734	arg1	increase					1736:1743	a statistically significant increase	1708:1743	a statistically significant increase of PT	1708:1749	At T₂and T₃in Group A and Group B, a statistically significant increase of PT was detected compared with the beginning of resuscitation.
26753645	5	14	theme	chloride	628:635	arg1	starch					650:655	sodium chloride hydroxyethyl starch 40	621:658	hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40)	610:676	The patients in Group B were treated with hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40).
26753645	9	15	dep	A.	1301:1302	arg1	detected					1354:1361	detected	1354:1361	were detected at T₁, T₂and T₃compared with T0 in Group A	1349:1404	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	5	16	theme	starch	650:655	arg1	injection					660:668	hypertonic sodium chloride hydroxyethyl starch 40 injection	610:668	hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40)	610:676	The patients in Group B were treated with hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40).
26753645	5	16	theme	starch	650:655	arg1	HSH40					671:675	HSH40	671:675	HSH40	671:675	The patients in Group B were treated with hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40).
26753645	7	17	theme	indicator	848:856	arg1	parameters					858:867	indicator parameters	848:867	indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP)	848:995	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	17	theme	indicator	848:856	arg1	hematocrit					896:905	hematocrit	896:905	hematocrit (HCT)	896:911	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	17	theme	indicator	848:856	arg1	time					926:929	prothrombin time	914:929	prothrombin time (PT)	914:934	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	17	theme	indicator	848:856	arg1	protein					983:989	C-reactive protein	972:989	C-reactive protein (CRP)	972:995	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	17	theme	indicator	848:856	arg1	hemoglobin					879:888	hemoglobin	879:888	hemoglobin (HB)	879:893	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	17	theme	indicator	848:856	arg1	acid					958:961	arterial blood lacic acid	937:961	arterial blood lacic acid (LA)	937:966	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	4	18	theme	Group	414:418	arg1	A					420:420	Group A	414:420	Group A	414:420	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	0	19	theme	traumatic	83:91	arg1	shock					93:97	traumatic shock	83:97	traumatic shock	83:97	Pre-hospital emergency values of hypertonic-hyperonconic limited resuscitation for traumatic shock.
26753645	10	20	theme	detected	1522:1529	arg1	smaller					1544:1550	smaller	1544:1550	smaller	1544:1550	In contrast, no significant difference was shown in detected HCT at T₂and T₃compared with T₀ in Group B, while the detected HB value was smaller.
26753645	10	20	theme	detected	1522:1529	arg1	value					1534:1538	the detected HB value	1518:1538	the detected HB value	1518:1538	In contrast, no significant difference was shown in detected HCT at T₂and T₃compared with T₀ in Group B, while the detected HB value was smaller.
26753645	2	21	with	patients	288:295	arg1	shock					312:316	traumatic shock	302:316	traumatic shock	302:316	The aim of this study is to investigate the effect of different resuscitation fluid compositions on the emergency resuscitation for patients with traumatic shock.
26753645	4	22	from	patients	402:409	arg1	A					420:420	Group A	414:420	Group A	414:420	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	5	23	theme	hypertonic	610:619	arg1	injection					660:668	hypertonic sodium chloride hydroxyethyl starch 40 injection	610:668	hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40)	610:676	The patients in Group B were treated with hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40).
26753645	5	23	theme	hypertonic	610:619	arg1	HSH40					671:675	HSH40	671:675	HSH40	671:675	The patients in Group B were treated with hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40).
26753645	4	24	theme	crystal	479:485	arg1	ratio					470:474	2:1 ratio	466:474	2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection)	466:565	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	9	25	theme	T₃after	1102:1108	arg1	T₂and					1096:1100	T₂and	1096:1100	T₂and	1096:1100	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	25	theme	T₃after	1102:1108	arg1	resuscitation					1116:1128	T₁, T₂and T₃after fluid resuscitation	1092:1128	resuscitation	1116:1128	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	26	from	patients	1154:1161	arg1	B					1172:1172	Group B	1166:1172	Group B	1166:1172	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	4	27	theme	fluid	454:458	arg1	resuscitation					440:452	resuscitation fluid	440:458	resuscitation fluid	440:458	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	1	28	theme	Traumatic	100:108	arg1	threat					129:134	a serious threat	119:134	a serious threat to life and health	119:153	Traumatic shock is a serious threat to life and health.
26753645	1	28	theme	Traumatic	100:108	arg1	shock					110:114	Traumatic shock	100:114	Traumatic shock	100:114	Traumatic shock is a serious threat to life and health.
26753645	0	29	theme	Pre-hospital	0:11	arg1	values					23:28	Pre-hospital emergency values	0:28	Pre-hospital emergency values of hypertonic-hyperonconic limited resuscitation for traumatic shock	0:97	Pre-hospital emergency values of hypertonic-hyperonconic limited resuscitation for traumatic shock.
26753645	2	30	theme	compositions	240:251	arg1	effect					200:205	the effect	196:205	the effect of different resuscitation fluid compositions on the emergency resuscitation for patients with traumatic shock	196:316	The aim of this study is to investigate the effect of different resuscitation fluid compositions on the emergency resuscitation for patients with traumatic shock.
26753645	7	31	theme	C-reactive	972:981	arg1	CRP					992:994	CRP	992:994	CRP	992:994	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	31	theme	C-reactive	972:981	arg1	protein					983:989	C-reactive protein	972:989	C-reactive protein (CRP)	972:995	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	5	32	from	patients	572:579	arg1	B					590:590	Group B	584:590	Group B	584:590	The patients in Group B were treated with hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40).
26753645	1	33	theme	serious	121:127	arg1	threat					129:134	a serious threat	119:134	a serious threat to life and health	119:153	Traumatic shock is a serious threat to life and health.
26753645	1	33	theme	serious	121:127	arg1	shock					110:114	Traumatic shock	100:114	Traumatic shock	100:114	Traumatic shock is a serious threat to life and health.
26753645	2	34	theme	study	172:176	arg1	aim					160:162	The aim	156:162	The aim of this study	156:176	The aim of this study is to investigate the effect of different resuscitation fluid compositions on the emergency resuscitation for patients with traumatic shock.
26753645	0	35	theme	hypertonic-hyperonconic	33:55	arg1	resuscitation					65:77	hypertonic-hyperonconic limited resuscitation	33:77	hypertonic-hyperonconic limited resuscitation	33:77	Pre-hospital emergency values of hypertonic-hyperonconic limited resuscitation for traumatic shock.
26753645	3	36	theme	Group	389:393	arg1	B					395:395	Group B	389:395	Group B	389:395	Sixty patients were enrolled and divided into two groups, Group A and Group B.
26753645	3	36	theme	Group	389:393	arg1	groups					369:374	two groups	365:374	two groups	365:374	Sixty patients were enrolled and divided into two groups, Group A and Group B.
26753645	4	37	theme	0.9	488:490	arg1	%					491:491	%	491:491	%	491:491	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	6	38	theme	fluid	700:704	arg1	dosage					706:711	fluid dosage	700:711	fluid dosage	700:711	Both vital signs and fluid dosage were monitored and recorded.
26753645	0	39	theme	resuscitation	65:77	arg1	values					23:28	Pre-hospital emergency values	0:28	Pre-hospital emergency values of hypertonic-hyperonconic limited resuscitation for traumatic shock	0:97	Pre-hospital emergency values of hypertonic-hyperonconic limited resuscitation for traumatic shock.
26753645	13	40	theme	Group	1854:1858	arg1	A					1860:1860	Group A	1854:1860	Group A	1854:1860	At T₂and T₃after resuscitation, CRP in both Group A and Group B was significantly increased compared with that upon admission to hospital, and was lower in Group B than in Group A.
26753645	4	41	theme	sodium	493:498	arg1	crystal					479:485	crystal	479:485	crystal (0.9% sodium chloride injection)	479:518	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	4	41	theme	sodium	493:498	arg1	injection					509:517	0.9% sodium chloride injection	488:517	0.9% sodium chloride injection	488:517	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	11	42	located	detected	1600:1607	arg1	T₂and					1616:1620	T₂and	1616:1620	T₂and	1616:1620	a statistically significant decrease of LA was detected at T₁, T₂and T₃in Group A and Group B compared with that at T₀.
26753645	11	42	located	detected	1600:1607	arg2	decrease					1581:1588	a statistically significant decrease	1553:1588	a statistically significant decrease of LA	1553:1594	a statistically significant decrease of LA was detected at T₁, T₂and T₃in Group A and Group B compared with that at T₀.
26753645	11	42	located	detected	1600:1607	arg1	T₃in					1622:1625	T₃in	1622:1625	T₃in	1622:1625	a statistically significant decrease of LA was detected at T₁, T₂and T₃in Group A and Group B compared with that at T₀.
26753645	13	43	theme	T₃after	1819:1825	arg1	resuscitation					1827:1839	T₂and T₃after resuscitation	1813:1839	T₂and T₃after resuscitation	1813:1839	At T₂and T₃after resuscitation, CRP in both Group A and Group B was significantly increased compared with that upon admission to hospital, and was lower in Group B than in Group A.
26753645	4	44	theme	injection	556:564	arg1	starch					546:551	hydroxyethyl starch	533:551	hydroxyethyl starch 40 injection	533:564	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	13	45	dep	hospital	1939:1946	arg1	to					1936:1937	to	1936:1937	to	1936:1937	At T₂and T₃after resuscitation, CRP in both Group A and Group B was significantly increased compared with that upon admission to hospital, and was lower in Group B than in Group A.
26753645	10	46	theme	detected	1459:1466	arg1	HCT					1468:1470	detected HCT	1459:1470	detected HCT	1459:1470	In contrast, no significant difference was shown in detected HCT at T₂and T₃compared with T₀ in Group B, while the detected HB value was smaller.
26753645	7	47	theme	blood	946:950	arg1	LA					964:965	LA	964:965	LA	964:965	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	47	theme	blood	946:950	arg1	acid					958:961	arterial blood lacic acid	937:961	arterial blood lacic acid (LA)	937:966	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	11	48	theme	significant	1569:1579	arg1	decrease					1581:1588	a statistically significant decrease	1553:1588	a statistically significant decrease of LA	1553:1594	a statistically significant decrease of LA was detected at T₁, T₂and T₃in Group A and Group B compared with that at T₀.
26753645	9	49	theme	Group	1199:1203	arg1	A					1205:1205	Group A	1199:1205	Group A	1199:1205	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	50	theme	Group	1398:1402	arg1	A					1404:1404	Group A	1398:1404	Group A	1398:1404	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	5	51	theme	Group	584:588	arg1	B					590:590	Group B	584:590	Group B	584:590	The patients in Group B were treated with hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40).
26753645	10	52	theme	Group	1503:1507	arg1	B					1509:1509	Group B	1503:1509	Group B	1503:1509	In contrast, no significant difference was shown in detected HCT at T₂and T₃compared with T₀ in Group B, while the detected HB value was smaller.
26753645	13	53	theme	Group	1966:1970	arg1	B					1972:1972	Group B	1966:1972	Group B	1966:1972	At T₂and T₃after resuscitation, CRP in both Group A and Group B was significantly increased compared with that upon admission to hospital, and was lower in Group B than in Group A.
26753645	8	54	theme	hemodynamic	1049:1059	arg1	profile					1061:1067	hemodynamic profile	1049:1067	hemodynamic profile	1049:1067	Tissue oxygenation and hemodynamic profile were also analyzed.
26753645	13	55	theme	Group	1982:1986	arg1	A					1988:1988	Group A	1982:1988	Group A	1982:1988	At T₂and T₃after resuscitation, CRP in both Group A and Group B was significantly increased compared with that upon admission to hospital, and was lower in Group B than in Group A.
26753645	9	56	theme	arterial	1228:1235	arg1	pressure					1237:1244	the average arterial pressure	1216:1244	the average arterial pressure in Group B	1216:1255	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	56	theme	arterial	1228:1235	arg1	higher					1275:1280	higher	1275:1280	higher	1275:1280	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	12	57	theme	PT	1748:1749	arg1	increase					1736:1743	a statistically significant increase	1708:1743	a statistically significant increase of PT	1708:1749	At T₂and T₃in Group A and Group B, a statistically significant increase of PT was detected compared with the beginning of resuscitation.
26753645	2	58	theme	fluid	234:238	arg1	compositions					240:251	different resuscitation fluid compositions	210:251	different resuscitation fluid compositions	210:251	The aim of this study is to investigate the effect of different resuscitation fluid compositions on the emergency resuscitation for patients with traumatic shock.
26753645	7	59	theme	resuscitation	762:774	arg1	min					801:803	60 min	798:803	60 min (T₂)	798:808	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	59	theme	resuscitation	762:774	arg1	T₂					806:807	T₂	806:807	T₂	806:807	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	59	theme	resuscitation	762:774	arg1	T₃					823:824	T₃	823:824	T₃	823:824	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	59	theme	resuscitation	762:774	arg1	T₁					793:794	T₁	793:794	T₁	793:794	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	59	theme	resuscitation	762:774	arg1	beginning					749:757	the beginning	745:757	the beginning of resuscitation (T₀)	745:779	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	59	theme	resuscitation	762:774	arg1	min					818:820	120 min	814:820	120 min (T₃)	814:825	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	7	59	theme	resuscitation	762:774	arg1	min					788:790	30 min	785:790	30 min (T₁)	785:795	At the beginning of resuscitation (T₀) and 30 min (T₁), 60 min (T₂) and 120 min (T₃) after resuscitation, indicator parameters including hemoglobin (HB), hematocrit (HCT), prothrombin time (PT), arterial blood lacic acid (LA) and C-reactive protein (CRP) were monitored and recorded.
26753645	5	60	theme	sodium	621:626	arg1	starch					650:655	sodium chloride hydroxyethyl starch 40	621:658	hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40)	610:676	The patients in Group B were treated with hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40).
26753645	2	61	theme	different	210:218	arg1	compositions					240:251	different resuscitation fluid compositions	210:251	different resuscitation fluid compositions	210:251	The aim of this study is to investigate the effect of different resuscitation fluid compositions on the emergency resuscitation for patients with traumatic shock.
26753645	3	62	theme	Sixty	319:323	arg1	patients					325:332	Sixty patients	319:332	Sixty patients	319:332	Sixty patients were enrolled and divided into two groups, Group A and Group B.
26753645	5	63	theme	hydroxyethyl	637:648	arg1	starch					650:655	sodium chloride hydroxyethyl starch 40	621:658	hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40)	610:676	The patients in Group B were treated with hypertonic sodium chloride hydroxyethyl starch 40 injection (HSH40).
26753645	2	64	theme	emergency	260:268	arg1	resuscitation					270:282	the emergency resuscitation	256:282	the emergency resuscitation for patients with traumatic shock	256:316	The aim of this study is to investigate the effect of different resuscitation fluid compositions on the emergency resuscitation for patients with traumatic shock.
26753645	13	65	theme	admission	1926:1934	arg1	hospital					1939:1946	admission to hospital	1926:1946	admission to hospital	1926:1946	At T₂and T₃after resuscitation, CRP in both Group A and Group B was significantly increased compared with that upon admission to hospital, and was lower in Group B than in Group A.
26753645	10	66	theme	HB	1531:1532	arg1	smaller					1544:1550	smaller	1544:1550	smaller	1544:1550	In contrast, no significant difference was shown in detected HCT at T₂and T₃compared with T₀ in Group B, while the detected HB value was smaller.
26753645	10	66	theme	HB	1531:1532	arg1	value					1534:1538	the detected HB value	1518:1538	the detected HB value	1518:1538	In contrast, no significant difference was shown in detected HCT at T₂and T₃compared with T₀ in Group B, while the detected HB value was smaller.
26753645	12	67	theme	Group	1699:1703	arg1	B					1705:1705	Group B	1699:1705	Group B	1699:1705	At T₂and T₃in Group A and Group B, a statistically significant increase of PT was detected compared with the beginning of resuscitation.
26753645	9	68	theme	T₁	1092:1093	arg1	T₂and					1096:1100	T₂and	1096:1100	T₂and	1096:1100	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	68	theme	T₁	1092:1093	arg1	resuscitation					1116:1128	T₁, T₂and T₃after fluid resuscitation	1092:1128	resuscitation	1116:1128	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	12	69	dep	T₃in	1682:1685	arg1	A					1693:1693	Group A	1687:1693	Group A	1687:1693	At T₂and T₃in Group A and Group B, a statistically significant increase of PT was detected compared with the beginning of resuscitation.
26753645	12	69	dep	T₃in	1682:1685	arg1	B					1705:1705	Group B	1699:1705	Group B	1699:1705	At T₂and T₃in Group A and Group B, a statistically significant increase of PT was detected compared with the beginning of resuscitation.
26753645	4	70	dep	colloid	524:530	arg1	starch					546:551	hydroxyethyl starch	533:551	hydroxyethyl starch 40 injection	533:564	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	9	71	theme	fluid	1110:1114	arg1	T₂and					1096:1100	T₂and	1096:1100	T₂and	1096:1100	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	71	theme	fluid	1110:1114	arg1	resuscitation					1116:1128	T₁, T₂and T₃after fluid resuscitation	1092:1128	resuscitation	1116:1128	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	72	from	rates	1141:1145	arg1	B					1172:1172	Group B	1166:1172	Group B	1166:1172	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	4	73	theme	2:1	466:468	arg1	ratio					470:474	2:1 ratio	466:474	2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection)	466:565	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	0	74	theme	emergency	13:21	arg1	values					23:28	Pre-hospital emergency values	0:28	Pre-hospital emergency values of hypertonic-hyperonconic limited resuscitation for traumatic shock	0:97	Pre-hospital emergency values of hypertonic-hyperonconic limited resuscitation for traumatic shock.
26753645	12	75	located	detected	1755:1762	arg2	increase					1736:1743	a statistically significant increase	1708:1743	a statistically significant increase of PT	1708:1749	At T₂and T₃in Group A and Group B, a statistically significant increase of PT was detected compared with the beginning of resuscitation.
26753645	12	75	located	detected	1755:1762	arg1	T₃in					1682:1685	T₃in	1682:1685	T₃in	1682:1685	At T₂and T₃in Group A and Group B, a statistically significant increase of PT was detected compared with the beginning of resuscitation.
26753645	9	76	theme	heart	1135:1139	arg1	rates					1141:1145	the heart rates	1131:1145	the heart rates of the patients in Group B	1131:1172	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	76	theme	heart	1135:1139	arg1	lower					1179:1183	lower	1179:1183	lower	1179:1183	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	77	theme	HB	1338:1339	arg1	decreases					1325:1333	significant decreases	1313:1333	significant decreases of HB and HCT	1313:1347	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	11	78	theme	Group	1639:1643	arg1	B					1645:1645	Group B	1639:1645	Group B	1639:1645	a statistically significant decrease of LA was detected at T₁, T₂and T₃in Group A and Group B compared with that at T₀.
26753645	3	79	theme	Group	377:381	arg1	A					383:383	Group A	377:383	Group A	377:383	Sixty patients were enrolled and divided into two groups, Group A and Group B.
26753645	3	79	theme	Group	377:381	arg1	groups					369:374	two groups	365:374	two groups	365:374	Sixty patients were enrolled and divided into two groups, Group A and Group B.
26753645	4	80	theme	colloid	524:530	arg1	ratio					470:474	2:1 ratio	466:474	2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection)	466:565	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	11	81	theme	Group	1627:1631	arg1	A					1633:1633	Group A	1627:1633	Group A	1627:1633	a statistically significant decrease of LA was detected at T₁, T₂and T₃in Group A and Group B compared with that at T₀.
26753645	0	82	theme	limited	57:63	arg1	resuscitation					65:77	hypertonic-hyperonconic limited resuscitation	33:77	hypertonic-hyperonconic limited resuscitation	33:77	Pre-hospital emergency values of hypertonic-hyperonconic limited resuscitation for traumatic shock.
26753645	9	83	theme	HCT	1345:1347	arg1	decreases					1325:1333	significant decreases	1313:1333	significant decreases of HB and HCT	1313:1347	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	4	84	theme	%	491:491	arg1	crystal					479:485	crystal	479:485	crystal (0.9% sodium chloride injection)	479:518	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	4	84	theme	%	491:491	arg1	injection					509:517	0.9% sodium chloride injection	488:517	0.9% sodium chloride injection	488:517	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	9	85	theme	patients	1154:1161	arg1	rates					1141:1145	the heart rates	1131:1145	the heart rates of the patients in Group B	1131:1172	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	85	theme	patients	1154:1161	arg1	lower					1179:1183	lower	1179:1183	lower	1179:1183	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	4	86	theme	chloride	500:507	arg1	crystal					479:485	crystal	479:485	crystal (0.9% sodium chloride injection)	479:518	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	4	86	theme	chloride	500:507	arg1	injection					509:517	0.9% sodium chloride injection	488:517	0.9% sodium chloride injection	488:517	The patients in Group A were treated with resuscitation fluid, with 2:1 ratio of crystal (0.9% sodium chloride injection) and colloid (hydroxyethyl starch 40 injection).
26753645	13	87	from	CRP	1842:1844	arg1	A					1860:1860	Group A	1854:1860	Group A	1854:1860	At T₂and T₃after resuscitation, CRP in both Group A and Group B was significantly increased compared with that upon admission to hospital, and was lower in Group B than in Group A.
26753645	13	87	from	CRP	1842:1844	arg1	B					1872:1872	Group B	1866:1872	Group B	1866:1872	At T₂and T₃after resuscitation, CRP in both Group A and Group B was significantly increased compared with that upon admission to hospital, and was lower in Group B than in Group A.
26753645	10	88	theme	significant	1423:1433	arg1	difference					1435:1444	no significant difference	1420:1444	no significant difference	1420:1444	In contrast, no significant difference was shown in detected HCT at T₂and T₃compared with T₀ in Group B, while the detected HB value was smaller.
26753645	9	89	theme	Group	1166:1170	arg1	B					1172:1172	Group B	1166:1172	Group B	1166:1172	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	90	from	B	1172:1172	arg1	rates					1141:1145	the heart rates	1131:1145	the heart rates of the patients in Group B	1131:1172	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	90	from	B	1172:1172	arg1	lower					1179:1183	lower	1179:1183	lower	1179:1183	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	91	located	detected	1354:1361	arg1	T₂and					1370:1374	T₂and	1370:1374	T₂and	1370:1374	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	91	located	detected	1354:1361	arg2	decreases					1325:1333	significant decreases	1313:1333	significant decreases of HB and HCT	1313:1347	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	91	located	detected	1354:1361	arg1	T₃compared					1376:1385	T₃compared	1376:1385	T₃compared	1376:1385	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	92	from	pressure	1237:1244	arg1	B					1255:1255	Group B	1249:1255	Group B	1249:1255	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	9	93	theme	significant	1313:1323	arg1	decreases					1325:1333	significant decreases	1313:1333	significant decreases of HB and HCT	1313:1347	At T₁, T₂and T₃after fluid resuscitation, the heart rates of the patients in Group B were lower than those in Group A, whereas the average arterial pressure in Group B was significantly higher than that in Group A. Notably, significant decreases of HB and HCT were detected at T₁, T₂and T₃compared with T0 in Group A.
26753645	12	94	theme	resuscitation	1795:1807	arg1	beginning					1782:1790	the beginning	1778:1790	the beginning of resuscitation	1778:1807	At T₂and T₃in Group A and Group B, a statistically significant increase of PT was detected compared with the beginning of resuscitation.
26753645	6	95	theme	vital	684:688	arg1	signs					690:694	vital signs	684:694	vital signs	684:694	Both vital signs and fluid dosage were monitored and recorded.
26753645	12	96	theme	Group	1687:1691	arg1	A					1693:1693	Group A	1687:1693	Group A	1687:1693	At T₂and T₃in Group A and Group B, a statistically significant increase of PT was detected compared with the beginning of resuscitation.
26753645	11	97	dep	T₃in	1622:1625	arg1	A					1633:1633	Group A	1627:1633	Group A	1627:1633	a statistically significant decrease of LA was detected at T₁, T₂and T₃in Group A and Group B compared with that at T₀.
26753645	11	97	dep	T₃in	1622:1625	arg1	B					1645:1645	Group B	1639:1645	Group B	1639:1645	a statistically significant decrease of LA was detected at T₁, T₂and T₃in Group A and Group B compared with that at T₀.
26753645	11	98	theme	LA	1593:1594	arg1	decrease					1581:1588	a statistically significant decrease	1553:1588	a statistically significant decrease of LA	1553:1594	a statistically significant decrease of LA was detected at T₁, T₂and T₃in Group A and Group B compared with that at T₀.
25952834	2	0	theme	new	458:460	arg1	products					474:481	new stabilizing products	458:481	new stabilizing products	458:481	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	3	1	theme	white	638:642	arg1	wines					644:648	red and white wines	630:648	red and white wines	630:648	The effectiveness of 4 different PASPs and 1 MTA added to red and white wines was compared using the mini-contact test and cold test.
25952834	1	2	theme	bitartrate	149:158	arg1	precipitations					121:134	the precipitations	117:134	the precipitations of potassium bitartrate	117:158	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	1	3	from	wines	290:294	arg1	stable					260:265	stable	260:265	stable	260:265	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	1	4	theme	red	327:329	arg1	wines					331:335	red wines	327:335	red wines	327:335	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	2	5	theme	salts	557:561	arg1	use					527:529	the use	523:529	the use of Na and K polyaspartate salts (PASPs)	523:569	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	1	6	contain	have	160:163	arg2	limits					170:175	some limits	165:175	some limits	165:175	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	1	6	contain	have	160:163	arg1	additives					75:83	All additives	71:83	All additives used to stabilize wines against the precipitations of potassium bitartrate	71:158	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	2	7	theme	polyaspartate	543:555	arg1	PASPs					564:568	PASPs	564:568	PASPs	564:568	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	2	7	theme	polyaspartate	543:555	arg1	salts					557:561	Na and K polyaspartate salts	534:561	Na and K polyaspartate salts (PASPs)	534:569	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	5	8	theme	similar	804:810	arg1	effect					824:829	a similar stabilizing effect	802:829	a similar stabilizing effect	802:829	The PASPs showed a similar stabilizing effect and a longer stability over time compared to MTA.
25952834	2	9	theme	tartaric	486:493	arg1	precipitations					495:508	tartaric precipitations	486:508	tartaric precipitations	486:508	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	2	10	from	effect	448:453	arg1	precipitations					495:508	tartaric precipitations	486:508	tartaric precipitations	486:508	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	3	11	theme	PASPs	605:609	arg1	effectiveness					576:588	The effectiveness	572:588	The effectiveness of 4 different PASPs and 1 MTA added to red and white wines	572:648	The effectiveness of 4 different PASPs and 1 MTA added to red and white wines was compared using the mini-contact test and cold test.
25952834	1	12	from	stable	260:265	arg1	wines					290:294	white wines	284:294	white wines	284:294	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	3	13	theme	red	630:632	arg1	wines					644:648	red and white wines	630:648	red and white wines	630:648	The effectiveness of 4 different PASPs and 1 MTA added to red and white wines was compared using the mini-contact test and cold test.
25952834	1	14	theme	metatartaric	178:189	arg1	MTA					197:199	MTA	197:199	MTA	197:199	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	1	14	theme	metatartaric	178:189	arg1	acid					191:194	metatartaric acid	178:194	metatartaric acid (MTA)	178:200	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	0	15	theme	polyaspartate	7:19	arg1	Use					0:2	Use	0:2	Use of polyaspartate as inhibitor of tartaric	0:44	Use of polyaspartate as inhibitor of tartaric precipitations in wines.
25952834	3	16	theme	different	595:603	arg1	PASPs					605:609	4 different PASPs	593:609	4 different PASPs	593:609	The effectiveness of 4 different PASPs and 1 MTA added to red and white wines was compared using the mini-contact test and cold test.
25952834	5	17	theme	stabilizing	812:822	arg1	effect					824:829	a similar stabilizing effect	802:829	a similar stabilizing effect	802:829	The PASPs showed a similar stabilizing effect and a longer stability over time compared to MTA.
25952834	3	18	theme	mini-contact	673:684	arg1	test					686:689	the mini-contact test	669:689	the mini-contact test	669:689	The effectiveness of 4 different PASPs and 1 MTA added to red and white wines was compared using the mini-contact test and cold test.
25952834	1	19	theme	variable	359:366	arg1	effectiveness					368:380	a variable effectiveness	357:380	a variable effectiveness	357:380	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	1	20	theme	white	284:288	arg1	wines					290:294	white wines	284:294	white wines	284:294	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	6	21	theme	tartaric	939:946	arg1	stabilization					948:960	wine tartaric stabilization	934:960	wine tartaric stabilization	934:960	PASPs can be considered interesting as additives for wine tartaric stabilization.
25952834	2	22	theme	K	541:541	arg1	PASPs					564:568	PASPs	564:568	PASPs	564:568	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	2	22	theme	K	541:541	arg1	salts					557:561	Na and K polyaspartate salts	534:561	Na and K polyaspartate salts (PASPs)	534:569	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	1	23	dep	effective	205:213	arg1	unstable					224:231	unstable	224:231	unstable	224:231	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	4	24	theme	products	747:754	arg1	effect					715:720	The dose effect	706:720	The dose effect	706:720	The dose effect and the stability of the products over time were also studied.
25952834	4	24	theme	products	747:754	arg1	stability					730:738	the stability	726:738	the stability of the products over time	726:764	The dose effect and the stability of the products over time were also studied.
25952834	0	25	theme	tartaric	37:44	arg1	inhibitor					24:32	inhibitor	24:32	inhibitor of tartaric	24:44	Use of polyaspartate as inhibitor of tartaric precipitations in wines.
25952834	3	26	theme	cold	695:698	arg1	test					700:703	cold test	695:703	cold test	695:703	The effectiveness of 4 different PASPs and 1 MTA added to red and white wines was compared using the mini-contact test and cold test.
25952834	7	27	theme	Further	963:969	arg1	work					971:974	Further work	963:974	Further work	963:974	Further work is in progress to better characterize their enological properties.
25952834	3	28	theme	MTA	617:619	arg1	effectiveness					576:588	The effectiveness	572:588	The effectiveness of 4 different PASPs and 1 MTA added to red and white wines	572:648	The effectiveness of 4 different PASPs and 1 MTA added to red and white wines was compared using the mini-contact test and cold test.
25952834	7	29	theme	enological	1020:1029	arg1	properties					1031:1040	their enological properties	1014:1040	their enological properties	1014:1040	Further work is in progress to better characterize their enological properties.
25952834	2	30	theme	Na	534:535	arg1	PASPs					564:568	PASPs	564:568	PASPs	564:568	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	2	30	theme	Na	534:535	arg1	salts					557:561	Na and K polyaspartate salts	534:561	Na and K polyaspartate salts (PASPs)	534:569	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	1	31	theme	wine	395:398	arg1	composition					400:410	wine composition	395:410	wine composition	395:410	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	6	32	theme	wine	934:937	arg1	stabilization					948:960	wine tartaric stabilization	934:960	wine tartaric stabilization	934:960	PASPs can be considered interesting as additives for wine tartaric stabilization.
25952834	4	33	theme	dose	710:713	arg1	effect					715:720	The dose effect	706:720	The dose effect	706:720	The dose effect and the stability of the products over time were also studied.
25952834	5	34	theme	longer	837:842	arg1	stability					844:852	a longer stability	835:852	a longer stability	835:852	The PASPs showed a similar stabilizing effect and a longer stability over time compared to MTA.
25952834	2	35	theme	products	474:481	arg1	effect					448:453	the effect	444:453	the effect of new stabilizing products on tartaric precipitations	444:508	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	1	36	contain	have	352:355	arg1	mannoproteins					338:350	mannoproteins	338:350	mannoproteins	338:350	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	1	36	contain	have	352:355	arg2	effectiveness					368:380	a variable effectiveness	357:380	a variable effectiveness	357:380	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	2	37	theme	stabilizing	462:472	arg1	products					474:481	new stabilizing products	458:481	new stabilizing products	458:481	This work was aimed at testing the effect of new stabilizing products on tartaric precipitations, focusing on the use of Na and K polyaspartate salts (PASPs).
25952834	1	38	theme	color	308:312	arg1	stability					314:322	color stability	308:322	color stability	308:322	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
25952834	1	39	theme	potassium	139:147	arg1	bitartrate					149:158	potassium bitartrate	139:158	potassium bitartrate	139:158	All additives used to stabilize wines against the precipitations of potassium bitartrate have some limits: metatartaric acid (MTA) is effective but very unstable, carboxymethylcellulose is stable and effective in white wines but affects color stability in red wines, mannoproteins have a variable effectiveness depending on wine composition.
24315808	8	0	theme	almond	892:897	arg1	consequence					867:877	a consequence	865:877	a consequence of almond or almond skin supplementation	865:918	were observed in fecal samples as a consequence of almond or almond skin supplementation.
24315808	4	1	theme	Fecal	493:497	arg1	samples					499:505	Fecal samples	493:505	Fecal samples	493:505	Fecal samples were collected at defined time points and analyzed for microbiota composition and selected indicators of microbial activity.
24315808	11	2	theme	skin	1385:1388	arg1	ingestion					1390:1398	almond skin ingestion	1378:1398	almond skin ingestion	1378:1398	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	12	3	contain	possess	1670:1676	arg1	skins					1664:1668	almonds and almond skins	1645:1668	almonds and almond skins	1645:1668	Thus we believe that almonds and almond skins possess potential prebiotic properties.
24315808	12	3	contain	possess	1670:1676	arg2	properties					1698:1707	potential prebiotic properties	1678:1707	potential prebiotic properties	1678:1707	Thus we believe that almonds and almond skins possess potential prebiotic properties.
24315808	10	4	theme	enzyme	1164:1169	arg1	activities					1171:1180	bacterial enzyme activities	1154:1180	bacterial enzyme activities	1154:1180	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	11	5	theme	factors	1577:1583	arg1	promotion					1546:1554	the promotion	1542:1554	the promotion of health beneficial factors	1542:1583	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	11	5	theme	factors	1577:1583	arg1	inhibition					1593:1602	the inhibition	1589:1602	the inhibition of harmful factors	1589:1621	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	4	6	theme	selected	589:596	arg1	indicators					598:607	selected indicators	589:607	selected indicators of microbial activity	589:629	Fecal samples were collected at defined time points and analyzed for microbiota composition and selected indicators of microbial activity.
24315808	11	7	theme	health	1559:1564	arg1	factors					1577:1583	health beneficial factors	1559:1583	health beneficial factors	1559:1583	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	5	8	theme	Different	632:640	arg1	strains					642:648	Different strains	632:648	Different strains of intestinal bacteria	632:671	Different strains of intestinal bacteria had varying degrees of growth sensitivity to almonds or almond skins.
24315808	2	9	theme	skin	275:278	arg1	intake					280:285	almond skin intake	268:285	almond skin intake	268:285	In this study we investigated the prebiotic effects of almond and almond skin intake in healthy humans.
24315808	1	10	theme	prebiotic	180:188	arg1	properties					190:199	potential prebiotic properties	170:199	potential prebiotic properties	170:199	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	9	11	theme	coli	961:964	arg1	populations					934:944	the populations	930:944	the populations of Escherichia coli	930:964	However, the populations of Escherichia coli did not change significantly, while the growth of the pathogen Clostridum perfringens was significantly repressed.
24315808	1	12	theme	almond	108:113	arg1	skins					115:119	Almonds and almond skins	96:119	Almonds and almond skins	96:119	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	11	13	theme	bacterial	1501:1509	arg1	activities					1511:1520	the intestinal bacterial activities	1486:1520	the intestinal bacterial activities	1486:1520	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	2	14	theme	prebiotic	236:244	arg1	effects					246:252	the prebiotic effects	232:252	the prebiotic effects of almond and almond skin intake in healthy humans	232:303	In this study we investigated the prebiotic effects of almond and almond skin intake in healthy humans.
24315808	12	15	theme	almonds	1645:1651	arg1	skins					1664:1668	almonds and almond skins	1645:1668	almonds and almond skins	1645:1668	Thus we believe that almonds and almond skins possess potential prebiotic properties.
24315808	8	16	dep	almond	882:887	arg1	supplementation					904:918	skin supplementation	899:918	skin supplementation	899:918	were observed in fecal samples as a consequence of almond or almond skin supplementation.
24315808	0	17	theme	intestinal	49:58	arg1	microbiota					60:69	intestinal microbiota	49:69	intestinal microbiota in healthy adult humans	49:93	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans.
24315808	3	18	theme	positive	462:469	arg1	control					471:477	positive control	462:477	positive control	462:477	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	4	19	theme	activity	622:629	arg1	composition					573:583	microbiota composition	562:583	microbiota composition	562:583	Fecal samples were collected at defined time points and analyzed for microbiota composition and selected indicators of microbial activity.
24315808	4	19	theme	activity	622:629	arg1	indicators					598:607	selected indicators	589:607	selected indicators of microbial activity	589:629	Fecal samples were collected at defined time points and analyzed for microbiota composition and selected indicators of microbial activity.
24315808	5	20	theme	sensitivity	703:713	arg1	degrees					685:691	varying degrees	677:691	varying degrees of growth sensitivity to almonds or almond skins	677:740	Different strains of intestinal bacteria had varying degrees of growth sensitivity to almonds or almond skins.
24315808	1	21	from	fiber	133:137	arg1	rich					125:128	rich	125:128	rich	125:128	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	3	22	theme	healthy	320:326	arg1	volunteers					334:343	48 healthy adult volunteers	317:343	48 healthy adult volunteers	317:343	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	10	23	theme	fecal	1222:1226	arg1	activity					1244:1251	fecal β-galactosidase activity	1222:1251	fecal β-galactosidase activity	1222:1251	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	0	24	theme	adult	82:86	arg1	humans					88:93	healthy adult humans	74:93	healthy adult humans	74:93	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans.
24315808	1	25	from	rich	125:128	arg1	fiber					133:137	fiber	133:137	fiber	133:137	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	1	25	from	rich	125:128	arg1	components					149:158	other components	143:158	other components	143:158	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	5	26	contain	had	673:675	arg1	strains					642:648	Different strains	632:648	Different strains of intestinal bacteria	632:671	Different strains of intestinal bacteria had varying degrees of growth sensitivity to almonds or almond skins.
24315808	5	26	contain	had	673:675	arg2	degrees					685:691	varying degrees	677:691	varying degrees of growth sensitivity to almonds or almond skins	677:740	Different strains of intestinal bacteria had varying degrees of growth sensitivity to almonds or almond skins.
24315808	10	27	theme	fecal	1270:1274	arg1	β-glucuronidase					1276:1290	fecal β-glucuronidase	1270:1290	fecal β-glucuronidase	1270:1290	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	5	28	theme	varying	677:683	arg1	degrees					685:691	varying degrees	677:691	varying degrees of growth sensitivity to almonds or almond skins	677:740	Different strains of intestinal bacteria had varying degrees of growth sensitivity to almonds or almond skins.
24315808	12	29	theme	potential	1678:1686	arg1	properties					1698:1707	potential prebiotic properties	1678:1707	potential prebiotic properties	1678:1707	Thus we believe that almonds and almond skins possess potential prebiotic properties.
24315808	9	30	theme	pathogen	1020:1027	arg1	growth					1006:1011	the growth	1002:1011	the growth of the pathogen Clostridum perfringens	1002:1050	However, the populations of Escherichia coli did not change significantly, while the growth of the pathogen Clostridum perfringens was significantly repressed.
24315808	0	31	theme	Prebiotic	0:8	arg1	effects					10:16	Prebiotic effects	0:16	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans	0:93	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans.
24315808	3	32	theme	volunteers	334:343	arg1	total					308:312	A total	306:312	A total of 48 healthy adult volunteers	306:343	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	1	33	theme	Almonds	96:102	arg1	skins					115:119	Almonds and almond skins	96:119	Almonds and almond skins	96:119	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	2	34	from	effects	246:252	arg1	humans					298:303	healthy humans	290:303	healthy humans	290:303	In this study we investigated the prebiotic effects of almond and almond skin intake in healthy humans.
24315808	3	35	theme	roasted	370:376	arg1	56 g					387:390	56 g	387:390	56 g	387:390	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	3	35	theme	roasted	370:376	arg1	almonds					378:384	roasted almonds	370:384	roasted almonds (56 g)	370:391	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	3	36	theme	almond	394:399	arg1	skins					401:405	almond skins	394:405	almond skins (10 g)	394:412	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	3	36	theme	almond	394:399	arg1	10 g					408:411	10 g	408:411	10 g	408:411	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	10	37	theme	composition	1123:1133	arg1	Modification					1081:1092	Modification	1081:1092	Modification of the intestinal microbiota composition	1081:1133	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	6	38	theme	spp	803:805	arg1	populations					772:782	the populations	768:782	the populations of Bifidobacterium spp	768:805	Significant increases in the populations of Bifidobacterium spp.
24315808	11	39	theme	harmful	1607:1613	arg1	factors					1615:1621	harmful factors	1607:1621	harmful factors	1607:1621	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	5	40	theme	almond	729:734	arg1	skins					736:740	almond skins	729:740	almond skins	729:740	Different strains of intestinal bacteria had varying degrees of growth sensitivity to almonds or almond skins.
24315808	10	41	theme	intestinal	1101:1110	arg1	composition					1123:1133	the intestinal microbiota composition	1097:1133	the intestinal microbiota composition	1097:1133	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	11	42	from	modification	1470:1481	arg1	profile					1456:1462	the intestinal microbiota profile	1430:1462	the intestinal microbiota profile	1430:1462	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	10	43	theme	bacterial	1154:1162	arg1	activities					1171:1180	bacterial enzyme activities	1154:1180	bacterial enzyme activities	1154:1180	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	8	44	theme	skin	899:902	arg1	supplementation					904:918	skin supplementation	899:918	skin supplementation	899:918	were observed in fecal samples as a consequence of almond or almond skin supplementation.
24315808	4	45	theme	defined	525:531	arg1	points					538:543	defined time points	525:543	defined time points	525:543	Fecal samples were collected at defined time points and analyzed for microbiota composition and selected indicators of microbial activity.
24315808	11	46	theme	almond	1378:1383	arg1	ingestion					1390:1398	almond skin ingestion	1378:1398	almond skin ingestion	1378:1398	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	5	47	theme	growth	696:701	arg1	sensitivity					703:713	growth sensitivity	696:713	growth sensitivity	696:713	Different strains of intestinal bacteria had varying degrees of growth sensitivity to almonds or almond skins.
24315808	3	48	theme	fructooligosaccharides	429:450	arg1	dose					362:365	a daily dose	354:365	a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks	354:490	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	6	49	theme	Significant	743:753	arg1	increases					755:763	Significant increases	743:763	Significant increases in the populations of Bifidobacterium spp.	743:806	Significant increases in the populations of Bifidobacterium spp.
24315808	2	50	theme	almond	268:273	arg1	intake					280:285	almond skin intake	268:285	almond skin intake	268:285	In this study we investigated the prebiotic effects of almond and almond skin intake in healthy humans.
24315808	2	51	theme	almond	257:262	arg1	effects					246:252	the prebiotic effects	232:252	the prebiotic effects of almond and almond skin intake in healthy humans	232:303	In this study we investigated the prebiotic effects of almond and almond skin intake in healthy humans.
24315808	1	52	theme	other	143:147	arg1	components					149:158	other components	143:158	other components	143:158	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	0	53	theme	skins	40:44	arg1	effects					10:16	Prebiotic effects	0:16	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans	0:93	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans.
24315808	10	54	from	increase	1210:1217	arg1	nitroreductase					1293:1306	nitroreductase	1293:1306	nitroreductase	1293:1306	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	10	54	from	increase	1210:1217	arg1	activities					1325:1334	azoreductase activities	1312:1334	azoreductase activities	1312:1334	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	10	54	from	increase	1210:1217	arg1	β-glucuronidase					1276:1290	fecal β-glucuronidase	1270:1290	fecal β-glucuronidase	1270:1290	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	10	54	from	increase	1210:1217	arg1	activity					1244:1251	fecal β-galactosidase activity	1222:1251	fecal β-galactosidase activity	1222:1251	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	4	55	theme	microbiota	562:571	arg1	composition					573:583	microbiota composition	562:583	microbiota composition	562:583	Fecal samples were collected at defined time points and analyzed for microbiota composition and selected indicators of microbial activity.
24315808	2	56	theme	healthy	290:296	arg1	humans					298:303	healthy humans	290:303	healthy humans	290:303	In this study we investigated the prebiotic effects of almond and almond skin intake in healthy humans.
24315808	11	57	theme	beneficial	1566:1575	arg1	factors					1577:1583	health beneficial factors	1559:1583	health beneficial factors	1559:1583	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	2	58	theme	intake	280:285	arg1	effects					246:252	the prebiotic effects	232:252	the prebiotic effects of almond and almond skin intake in healthy humans	232:303	In this study we investigated the prebiotic effects of almond and almond skin intake in healthy humans.
24315808	1	59	theme	potential	170:178	arg1	properties					190:199	potential prebiotic properties	170:199	potential prebiotic properties	170:199	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	11	60	theme	activities	1511:1520	arg1	modification					1470:1481	a modification	1468:1481	a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors	1468:1621	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	11	60	theme	activities	1511:1520	arg1	improvement					1415:1425	an improvement	1412:1425	an improvement in the intestinal microbiota profile	1412:1462	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	0	61	from	microbiota	60:69	arg1	humans					88:93	healthy adult humans	74:93	healthy adult humans	74:93	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans.
24315808	10	62	from	decreases	1257:1265	arg1	nitroreductase					1293:1306	nitroreductase	1293:1306	nitroreductase	1293:1306	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	10	62	from	decreases	1257:1265	arg1	activities					1325:1334	azoreductase activities	1312:1334	azoreductase activities	1312:1334	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	10	62	from	decreases	1257:1265	arg1	β-glucuronidase					1276:1290	fecal β-glucuronidase	1270:1290	fecal β-glucuronidase	1270:1290	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	10	62	from	decreases	1257:1265	arg1	activity					1244:1251	fecal β-galactosidase activity	1222:1251	fecal β-galactosidase activity	1222:1251	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	11	63	from	improvement	1415:1425	arg1	profile					1456:1462	the intestinal microbiota profile	1430:1462	the intestinal microbiota profile	1430:1462	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	11	64	theme	intestinal	1490:1499	arg1	activities					1511:1520	the intestinal bacterial activities	1486:1520	the intestinal bacterial activities	1486:1520	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	10	65	theme	significant	1198:1208	arg1	increase					1210:1217	a significant increase	1196:1217	a significant increase in fecal β-galactosidase activity	1196:1251	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	5	66	theme	bacteria	664:671	arg1	strains					642:648	Different strains	632:648	Different strains of intestinal bacteria	632:671	Different strains of intestinal bacteria had varying degrees of growth sensitivity to almonds or almond skins.
24315808	0	67	theme	healthy	74:80	arg1	humans					88:93	healthy adult humans	74:93	healthy adult humans	74:93	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans.
24315808	1	68	from	components	149:158	arg1	rich					125:128	rich	125:128	rich	125:128	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	12	69	theme	prebiotic	1688:1696	arg1	properties					1698:1707	potential prebiotic properties	1678:1707	potential prebiotic properties	1678:1707	Thus we believe that almonds and almond skins possess potential prebiotic properties.
24315808	10	70	theme	azoreductase	1312:1323	arg1	activities					1325:1334	azoreductase activities	1312:1334	azoreductase activities	1312:1334	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	0	71	from	effects	10:16	arg1	microbiota					60:69	intestinal microbiota	49:69	intestinal microbiota in healthy adult humans	49:93	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans.
24315808	4	72	theme	microbial	612:620	arg1	activity					622:629	microbial activity	612:629	microbial activity	612:629	Fecal samples were collected at defined time points and analyzed for microbiota composition and selected indicators of microbial activity.
24315808	10	73	theme	β-galactosidase	1228:1242	arg1	activity					1244:1251	fecal β-galactosidase activity	1222:1251	fecal β-galactosidase activity	1222:1251	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	5	74	theme	intestinal	653:662	arg1	bacteria					664:671	intestinal bacteria	653:671	intestinal bacteria	653:671	Different strains of intestinal bacteria had varying degrees of growth sensitivity to almonds or almond skins.
24315808	3	75	theme	adult	328:332	arg1	volunteers					334:343	48 healthy adult volunteers	317:343	48 healthy adult volunteers	317:343	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	11	76	theme	intestinal	1434:1443	arg1	profile					1456:1462	the intestinal microbiota profile	1430:1462	the intestinal microbiota profile	1430:1462	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	12	77	theme	almond	1657:1662	arg1	skins					1664:1668	almonds and almond skins	1645:1668	almonds and almond skins	1645:1668	Thus we believe that almonds and almond skins possess potential prebiotic properties.
24315808	6	78	from	increases	755:763	arg1	populations					772:782	the populations	768:782	the populations of Bifidobacterium spp	768:805	Significant increases in the populations of Bifidobacterium spp.
24315808	3	79	theme	daily	356:360	arg1	dose					362:365	a daily dose	354:365	a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks	354:490	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	9	80	theme	Clostridum	1029:1038	arg1	pathogen					1020:1027	the pathogen Clostridum perfringens	1016:1050	the pathogen Clostridum perfringens	1016:1050	However, the populations of Escherichia coli did not change significantly, while the growth of the pathogen Clostridum perfringens was significantly repressed.
24315808	0	81	theme	almonds	21:27	arg1	effects					10:16	Prebiotic effects	0:16	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans	0:93	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans.
24315808	1	82	contain	have	165:168	arg2	properties					190:199	potential prebiotic properties	170:199	potential prebiotic properties	170:199	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	1	82	contain	have	165:168	arg1	fiber					133:137	fiber	133:137	fiber	133:137	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	1	82	contain	have	165:168	arg1	components					149:158	other components	143:158	other components	143:158	Almonds and almond skins are rich in fiber and other components that have potential prebiotic properties.
24315808	0	83	theme	almond	33:38	arg1	skins					40:44	almond skins	33:44	almond skins	33:44	Prebiotic effects of almonds and almond skins on intestinal microbiota in healthy adult humans.
24315808	11	84	theme	microbiota	1445:1454	arg1	profile					1456:1462	the intestinal microbiota profile	1430:1462	the intestinal microbiota profile	1430:1462	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	8	85	theme	fecal	848:852	arg1	samples					854:860	fecal samples	848:860	fecal samples	848:860	were observed in fecal samples as a consequence of almond or almond skin supplementation.
24315808	3	86	theme	almonds	378:384	arg1	dose					362:365	a daily dose	354:365	a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks	354:490	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	3	87	theme	skins	401:405	arg1	dose					362:365	a daily dose	354:365	a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks	354:490	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	10	88	theme	microbiota	1112:1121	arg1	composition					1123:1133	the intestinal microbiota composition	1097:1133	the intestinal microbiota composition	1097:1133	Modification of the intestinal microbiota composition induced changes in bacterial enzyme activities, specifically a significant increase in fecal β-galactosidase activity and decreases in fecal β-glucuronidase, nitroreductase and azoreductase activities.
24315808	6	89	theme	Bifidobacterium	787:801	arg1	spp					803:805	Bifidobacterium spp	787:805	Bifidobacterium spp	787:805	Significant increases in the populations of Bifidobacterium spp.
24315808	9	90	dep	pathogen	1020:1027	arg1	perfringens					1040:1050	the pathogen Clostridum perfringens	1016:1050	the pathogen Clostridum perfringens	1016:1050	However, the populations of Escherichia coli did not change significantly, while the growth of the pathogen Clostridum perfringens was significantly repressed.
24315808	3	91	theme	commercial	418:427	arg1	8 g					453:455	8 g	453:455	8 g	453:455	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	3	91	theme	commercial	418:427	arg1	fructooligosaccharides					429:450	commercial fructooligosaccharides	418:450	commercial fructooligosaccharides (8 g) (as positive control)	418:478	A total of 48 healthy adult volunteers consumed a daily dose of roasted almonds (56 g), almond skins (10 g), or commercial fructooligosaccharides (8 g) (as positive control) for 6 weeks.
24315808	8	92	theme	almond	882:887	arg1	consequence					867:877	a consequence	865:877	a consequence of almond or almond skin supplementation	865:918	were observed in fecal samples as a consequence of almond or almond skin supplementation.
24315808	4	93	theme	time	533:536	arg1	points					538:543	defined time points	525:543	defined time points	525:543	Fecal samples were collected at defined time points and analyzed for microbiota composition and selected indicators of microbial activity.
24315808	11	94	theme	factors	1615:1621	arg1	promotion					1546:1554	the promotion	1542:1554	the promotion of health beneficial factors	1542:1583	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24315808	11	94	theme	factors	1615:1621	arg1	inhibition					1593:1602	the inhibition	1589:1602	the inhibition of harmful factors	1589:1621	Our observations suggest that almond and almond skin ingestion may lead to an improvement in the intestinal microbiota profile and a modification of the intestinal bacterial activities, which would induce the promotion of health beneficial factors and the inhibition of harmful factors.
24862440	5	0	theme	in	884:885	arg1	testing					892:898	extensive in vitro and in vivo testing	861:898	extensive in vitro and in vivo testing	861:898	Most importantly, these non-covalently cross-linked composites are surprisingly robust yet biodegradable upon extensive in vitro and in vivo testing and show bone interactive capacity evidenced by bone ingrowth into material remnants.
24862440	5	1	theme	in	871:872	arg1	testing					892:898	extensive in vitro and in vivo testing	861:898	extensive in vitro and in vivo testing	861:898	Most importantly, these non-covalently cross-linked composites are surprisingly robust yet biodegradable upon extensive in vitro and in vivo testing and show bone interactive capacity evidenced by bone ingrowth into material remnants.
24862440	6	2	theme	reversible	1137:1146	arg1	bonds					1148:1152	reversible bonds	1137:1152	reversible bonds	1137:1152	The herein presented method provides a new methodology for constructing nanoscale composites for biomedical applications, which owe their integrity to reversible bonds.
24862440	2	3	theme	strong	387:392	arg1	materials					394:402	strong materials	387:402	strong materials	387:402	However, the low binding energy of such reversible interactions can be compensated by increasing their number to work in concert to create strong materials.
24862440	6	4	theme	new	1025:1027	arg1	methodology					1029:1039	a new methodology	1023:1039	a new methodology for constructing nanoscale composites for biomedical applications, which owe their integrity to reversible bonds	1023:1152	The herein presented method provides a new methodology for constructing nanoscale composites for biomedical applications, which owe their integrity to reversible bonds.
24862440	5	5	theme	extensive	861:869	arg1	testing					892:898	extensive in vitro and in vivo testing	861:898	extensive in vitro and in vivo testing	861:898	Most importantly, these non-covalently cross-linked composites are surprisingly robust yet biodegradable upon extensive in vitro and in vivo testing and show bone interactive capacity evidenced by bone ingrowth into material remnants.
24862440	5	6	link	cross-linked	790:801	arg1	composites					803:812	these non-covalently cross-linked composites	769:812	these non-covalently cross-linked composites	769:812	Most importantly, these non-covalently cross-linked composites are surprisingly robust yet biodegradable upon extensive in vitro and in vivo testing and show bone interactive capacity evidenced by bone ingrowth into material remnants.
24862440	5	7	theme	interactive	914:924	arg1	capacity					926:933	bone interactive capacity	909:933	bone interactive capacity evidenced by bone ingrowth into material remnants	909:983	Most importantly, these non-covalently cross-linked composites are surprisingly robust yet biodegradable upon extensive in vitro and in vivo testing and show bone interactive capacity evidenced by bone ingrowth into material remnants.
24862440	1	8	theme	substantial	212:222	arg1	integrity					224:232	substantial integrity	212:232	substantial integrity	212:232	Non-covalent interactions are often regarded as insufficient to construct macroscopic materials of substantial integrity and cohesion.
24862440	5	9	theme	bone	909:912	arg1	capacity					926:933	bone interactive capacity	909:933	bone interactive capacity evidenced by bone ingrowth into material remnants	909:983	Most importantly, these non-covalently cross-linked composites are surprisingly robust yet biodegradable upon extensive in vitro and in vivo testing and show bone interactive capacity evidenced by bone ingrowth into material remnants.
24862440	0	10	theme	Self-healing	0:11	arg1	nanocomposites					20:33	Self-healing hybrid nanocomposites	0:33	Self-healing hybrid nanocomposites	0:33	Self-healing hybrid nanocomposites consisting of bisphosphonated hyaluronan and calcium phosphate nanoparticles.
24862440	3	11	theme	hydrogel	489:496	arg1	development					436:446	the successful development	421:446	the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid	421:612	Here we present the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid.
24862440	0	12	theme	hybrid	13:18	arg1	nanocomposites					20:33	Self-healing hybrid nanocomposites	0:33	Self-healing hybrid nanocomposites	0:33	Self-healing hybrid nanocomposites consisting of bisphosphonated hyaluronan and calcium phosphate nanoparticles.
24862440	6	13	theme	presented	997:1005	arg1	method					1007:1012	The herein presented method	986:1012	The herein presented method	986:1012	The herein presented method provides a new methodology for constructing nanoscale composites for biomedical applications, which owe their integrity to reversible bonds.
24862440	1	14	theme	Non-covalent	113:124	arg1	insufficient					161:172	insufficient	161:172	insufficient	161:172	Non-covalent interactions are often regarded as insufficient to construct macroscopic materials of substantial integrity and cohesion.
24862440	1	14	theme	Non-covalent	113:124	arg1	interactions					126:137	Non-covalent interactions	113:137	Non-covalent interactions	113:137	Non-covalent interactions are often regarded as insufficient to construct macroscopic materials of substantial integrity and cohesion.
24862440	3	15	theme	successful	425:434	arg1	development					436:446	the successful development	421:446	the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid	421:612	Here we present the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid.
24862440	3	16	dep	injectable	454:463	arg1	cohesive					466:473	cohesive	466:473	cohesive	466:473	Here we present the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid.
24862440	1	17	theme	cohesion	238:245	arg1	materials					199:207	macroscopic materials	187:207	macroscopic materials of substantial integrity and cohesion	187:245	Non-covalent interactions are often regarded as insufficient to construct macroscopic materials of substantial integrity and cohesion.
24862440	5	18	dep	in	884:885	arg1	vivo					887:890	vivo	887:890	vivo	887:890	Most importantly, these non-covalently cross-linked composites are surprisingly robust yet biodegradable upon extensive in vitro and in vivo testing and show bone interactive capacity evidenced by bone ingrowth into material remnants.
24862440	3	19	theme	reversible	507:516	arg1	bonds					518:522	reversible bonds	507:522	reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid	507:612	Here we present the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid.
24862440	3	20	theme	bisphosphonate-functionalized	568:596	arg1	acid					609:612	bisphosphonate-functionalized hyaluronic acid	568:612	bisphosphonate-functionalized hyaluronic acid	568:612	Here we present the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid.
24862440	0	21	theme	bisphosphonated	49:63	arg1	nanoparticles					98:110	bisphosphonated hyaluronan and calcium phosphate nanoparticles	49:110	bisphosphonated hyaluronan and calcium phosphate nanoparticles	49:110	Self-healing hybrid nanocomposites consisting of bisphosphonated hyaluronan and calcium phosphate nanoparticles.
24862440	3	22	theme	nanocomposite	475:487	arg1	hydrogel					489:496	an injectable, cohesive nanocomposite hydrogel	451:496	an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid	451:612	Here we present the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid.
24862440	6	23	theme	biomedical	1083:1092	arg1	applications					1094:1105	biomedical applications	1083:1105	biomedical applications	1083:1105	The herein presented method provides a new methodology for constructing nanoscale composites for biomedical applications, which owe their integrity to reversible bonds.
24862440	3	24	theme	injectable	454:463	arg1	hydrogel					489:496	an injectable, cohesive nanocomposite hydrogel	451:496	an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid	451:612	Here we present the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid.
24862440	5	25	theme	material	967:974	arg1	remnants					976:983	material remnants	967:983	material remnants	967:983	Most importantly, these non-covalently cross-linked composites are surprisingly robust yet biodegradable upon extensive in vitro and in vivo testing and show bone interactive capacity evidenced by bone ingrowth into material remnants.
24862440	2	26	theme	binding	265:271	arg1	energy					273:278	the low binding energy	257:278	the low binding energy of such reversible interactions	257:310	However, the low binding energy of such reversible interactions can be compensated by increasing their number to work in concert to create strong materials.
24862440	3	27	theme	phosphate	540:548	arg1	nanoparticles					550:562	calcium phosphate nanoparticles	532:562	calcium phosphate nanoparticles	532:562	Here we present the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid.
24862440	0	28	theme	hyaluronan	65:74	arg1	nanoparticles					98:110	bisphosphonated hyaluronan and calcium phosphate nanoparticles	49:110	bisphosphonated hyaluronan and calcium phosphate nanoparticles	49:110	Self-healing hybrid nanocomposites consisting of bisphosphonated hyaluronan and calcium phosphate nanoparticles.
24862440	5	29	dep	in	871:872	arg1	vitro					874:878	vitro	874:878	vitro	874:878	Most importantly, these non-covalently cross-linked composites are surprisingly robust yet biodegradable upon extensive in vitro and in vivo testing and show bone interactive capacity evidenced by bone ingrowth into material remnants.
24862440	2	30	theme	low	261:263	arg1	energy					273:278	the low binding energy	257:278	the low binding energy of such reversible interactions	257:310	However, the low binding energy of such reversible interactions can be compensated by increasing their number to work in concert to create strong materials.
24862440	6	31	theme	nanoscale	1058:1066	arg1	composites					1068:1077	nanoscale composites	1058:1077	nanoscale composites	1058:1077	The herein presented method provides a new methodology for constructing nanoscale composites for biomedical applications, which owe their integrity to reversible bonds.
24862440	5	32	theme	cross-linked	790:801	arg1	composites					803:812	these non-covalently cross-linked composites	769:812	these non-covalently cross-linked composites	769:812	Most importantly, these non-covalently cross-linked composites are surprisingly robust yet biodegradable upon extensive in vitro and in vivo testing and show bone interactive capacity evidenced by bone ingrowth into material remnants.
24862440	4	33	theme	mineral	699:705	arg1	hydroxyapatite					735:748	hydroxyapatite	735:748	hydroxyapatite	735:748	These nanocomposites display a capacity for self-healing as well as adhesiveness to mineral surfaces such as enamel and hydroxyapatite.
24862440	4	33	theme	mineral	699:705	arg1	surfaces					707:714	mineral surfaces	699:714	mineral surfaces such as enamel and hydroxyapatite	699:748	These nanocomposites display a capacity for self-healing as well as adhesiveness to mineral surfaces such as enamel and hydroxyapatite.
24862440	4	33	theme	mineral	699:705	arg1	enamel					724:729	enamel	724:729	enamel	724:729	These nanocomposites display a capacity for self-healing as well as adhesiveness to mineral surfaces such as enamel and hydroxyapatite.
24862440	2	34	theme	interactions	299:310	arg1	energy					273:278	the low binding energy	257:278	the low binding energy of such reversible interactions	257:310	However, the low binding energy of such reversible interactions can be compensated by increasing their number to work in concert to create strong materials.
24862440	3	35	theme	calcium	532:538	arg1	nanoparticles					550:562	calcium phosphate nanoparticles	532:562	calcium phosphate nanoparticles	532:562	Here we present the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid.
24862440	0	36	theme	phosphate	88:96	arg1	nanoparticles					98:110	bisphosphonated hyaluronan and calcium phosphate nanoparticles	49:110	bisphosphonated hyaluronan and calcium phosphate nanoparticles	49:110	Self-healing hybrid nanocomposites consisting of bisphosphonated hyaluronan and calcium phosphate nanoparticles.
24862440	2	37	theme	reversible	288:297	arg1	interactions					299:310	such reversible interactions	283:310	such reversible interactions	283:310	However, the low binding energy of such reversible interactions can be compensated by increasing their number to work in concert to create strong materials.
24862440	5	38	theme	bone	948:951	arg1	ingrowth					953:960	bone ingrowth	948:960	bone ingrowth into material remnants	948:983	Most importantly, these non-covalently cross-linked composites are surprisingly robust yet biodegradable upon extensive in vitro and in vivo testing and show bone interactive capacity evidenced by bone ingrowth into material remnants.
24862440	3	39	theme	hyaluronic	598:607	arg1	acid					609:612	bisphosphonate-functionalized hyaluronic acid	568:612	bisphosphonate-functionalized hyaluronic acid	568:612	Here we present the successful development of an injectable, cohesive nanocomposite hydrogel based on reversible bonds between calcium phosphate nanoparticles and bisphosphonate-functionalized hyaluronic acid.
24862440	1	40	theme	integrity	224:232	arg1	materials					199:207	macroscopic materials	187:207	macroscopic materials of substantial integrity and cohesion	187:245	Non-covalent interactions are often regarded as insufficient to construct macroscopic materials of substantial integrity and cohesion.
24862440	0	41	theme	calcium	80:86	arg1	phosphate					88:96	calcium phosphate	80:96	calcium phosphate	80:96	Self-healing hybrid nanocomposites consisting of bisphosphonated hyaluronan and calcium phosphate nanoparticles.
24862440	2	42	theme	such	283:286	arg1	interactions					299:310	such reversible interactions	283:310	such reversible interactions	283:310	However, the low binding energy of such reversible interactions can be compensated by increasing their number to work in concert to create strong materials.
24862440	1	43	theme	macroscopic	187:197	arg1	materials					199:207	macroscopic materials	187:207	macroscopic materials of substantial integrity and cohesion	187:245	Non-covalent interactions are often regarded as insufficient to construct macroscopic materials of substantial integrity and cohesion.
27748576	5	0	theme	pores	694:698	arg1	number					676:681	the number	672:681	the number of smaller pores on increasing BP content	672:723	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	7	1	dep	In	974:975	arg1	vitro					977:981	vitro	977:981	vitro	977:981	In vitro tests are carried out using cocultures of osteoblast (OB) and osteoclast (OC).
27748576	9	2	theme	bone	1416:1419	arg1	processes					1428:1436	bone repair processes	1416:1436	bone repair processes	1416:1436	The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.
27748576	1	3	theme	metabolic	143:151	arg1	diseases					153:160	metabolic diseases	143:160	metabolic diseases characterized by abnormal bone loss	143:196	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	6	4	theme	drug	923:926	arg1	amount					909:914	a significant amount	895:914	a significant amount of the drug	895:926	The scaffolds exhibit a sustained CaAL•H2 O release profile, and a significant amount of the drug is retained in the scaffolds even after 14 d.
27748576	6	4	theme	drug	923:926	arg1	drug					923:926	the drug	919:926	the drug	919:926	The scaffolds exhibit a sustained CaAL•H2 O release profile, and a significant amount of the drug is retained in the scaffolds even after 14 d.
27748576	8	5	theme	polymerase	1187:1196	arg1	reaction					1204:1211	quantitative polymerase chain reaction	1174:1211	quantitative polymerase chain reaction	1174:1211	The evaluation of differentiation markers is performed both on the supernatants of cell culture and by means of quantitative polymerase chain reaction.
27748576	7	6	theme	osteoblast	1025:1034	arg1	cocultures					1011:1020	cocultures	1011:1020	cocultures of osteoblast (OB) and osteoclast (OC)	1011:1059	In vitro tests are carried out using cocultures of osteoblast (OB) and osteoclast (OC).
27748576	1	7	theme	diseases	153:160	arg1	treatment					130:138	the treatment	126:138	the treatment of metabolic diseases characterized by abnormal bone loss	126:196	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	3	8	theme	interconnected	511:524	arg1	%					539:539	>94%	536:539	>94%	536:539	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	3	8	theme	interconnected	511:524	arg1	porosity					526:533	a relevant interconnected porosity	500:533	a relevant interconnected porosity (>94%)	500:540	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	5	9	theme	strength	820:827	arg1	increase					771:778	the parallel significant increase	746:778	the parallel significant increase of the compressive modulus and collapse strength	746:827	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	5	10	with	agreement	731:739	arg1	increase					771:778	the parallel significant increase	746:778	the parallel significant increase of the compressive modulus and collapse strength	746:827	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	2	11	link	cross-linked	349:360	arg1	systems					405:411	delivery systems	396:411	delivery systems of calcium alendronate monohydrate (CaAL•H2 O)	396:458	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	11	link	cross-linked	349:360	arg1	scaffolds					370:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	6	12	theme	O	872:872	arg1	profile					882:888	a sustained CaAL•H2 O release profile	852:888	a sustained CaAL•H2 O release profile	852:888	The scaffolds exhibit a sustained CaAL•H2 O release profile, and a significant amount of the drug is retained in the scaffolds even after 14 d.
27748576	0	13	theme	Gelatin	0:6	arg1	Porous					8:13	Gelatin Porous	0:13	Gelatin Porous	0:13	Gelatin Porous Scaffolds as Delivery Systems of Calcium Alendronate.
27748576	9	14	theme	containing	1243:1252	arg1	scaffolds					1254:1262	BP containing scaffolds	1240:1262	BP containing scaffolds	1240:1262	The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.
27748576	8	15	theme	cell	1145:1148	arg1	culture					1150:1156	cell culture	1145:1156	cell culture	1145:1156	The evaluation of differentiation markers is performed both on the supernatants of cell culture and by means of quantitative polymerase chain reaction.
27748576	9	16	dep	support	1264:1270	arg1	whereas					1318:1324	whereas	1318:1324	whereas	1318:1324	The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.
27748576	5	17	theme	significant	759:769	arg1	increase					771:778	the parallel significant increase	746:778	the parallel significant increase of the compressive modulus and collapse strength	746:827	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	3	18	theme	3D	465:466	arg1	scaffolds					482:490	The 3D highly porous scaffolds	461:490	The 3D highly porous scaffolds	461:490	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	9	19	from	role	1408:1411	arg1	processes					1428:1436	bone repair processes	1416:1436	bone repair processes	1416:1436	The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.
27748576	5	20	theme	modulus	799:805	arg1	increase					771:778	the parallel significant increase	746:778	the parallel significant increase of the compressive modulus and collapse strength	746:827	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	2	21	theme	delivery	396:403	arg1	systems					405:411	delivery systems	396:411	delivery systems of calcium alendronate monohydrate (CaAL•H2 O)	396:458	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	21	theme	delivery	396:403	arg1	scaffolds					370:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	5	22	theme	number	676:681	arg1	increase					660:667	The relative increase	647:667	The relative increase of the number of smaller pores on increasing BP content	647:723	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	9	23	theme	repair	1421:1426	arg1	processes					1428:1436	bone repair processes	1416:1436	bone repair processes	1416:1436	The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.
27748576	1	24	theme	systemic	73:80	arg1	administration					82:95	The systemic administration	69:95	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss	69:196	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	5	25	theme	compressive	787:797	arg1	modulus					799:805	the compressive modulus	783:805	the compressive modulus	783:805	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	1	26	theme	abnormal	179:186	arg1	loss					193:196	abnormal bone loss	179:196	abnormal bone loss	179:196	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	2	27	theme	CaAL•H2	449:455	arg1	monohydrate					436:446	calcium alendronate monohydrate	416:446	calcium alendronate monohydrate (CaAL•H2 O)	416:458	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	27	theme	CaAL•H2	449:455	arg1	O					457:457	CaAL•H2 O	449:457	CaAL•H2 O	449:457	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	6	28	theme	significant	897:907	arg1	amount					909:914	a significant amount	895:914	a significant amount of the drug	895:926	The scaffolds exhibit a sustained CaAL•H2 O release profile, and a significant amount of the drug is retained in the scaffolds even after 14 d.
27748576	6	28	theme	significant	897:907	arg1	drug					923:926	the drug	919:926	the drug	919:926	The scaffolds exhibit a sustained CaAL•H2 O release profile, and a significant amount of the drug is retained in the scaffolds even after 14 d.
27748576	3	29	theme	CaAL•H2	562:568	arg1	%					595:595	0, 3, and 6 wt%	581:595	0, 3, and 6 wt%	581:595	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	3	29	theme	CaAL•H2	562:568	arg1	content					572:578	CaAL•H2 O content	562:578	CaAL•H2 O content (0, 3, and 6 wt%)	562:596	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	1	30	theme	bone	188:191	arg1	loss					193:196	abnormal bone loss	179:196	abnormal bone loss	179:196	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	7	31	theme	osteoclast	1045:1054	arg1	cocultures					1011:1020	cocultures	1011:1020	cocultures of osteoblast (OB) and osteoclast (OC)	1011:1059	In vitro tests are carried out using cocultures of osteoblast (OB) and osteoclast (OC).
27748576	7	32	theme	In	974:975	arg1	tests					983:987	In vitro tests	974:987	In vitro tests	974:987	In vitro tests are carried out using cocultures of osteoblast (OB) and osteoclast (OC).
27748576	9	33	theme	osteoclast	1339:1348	arg1	viability					1350:1358	osteoclast viability	1339:1358	osteoclast viability	1339:1358	The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.
27748576	1	34	theme	bisphosphonates	100:114	arg1	administration					82:95	The systemic administration	69:95	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss	69:196	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	2	35	theme	alendronate	424:434	arg1	monohydrate					436:446	calcium alendronate monohydrate	416:446	calcium alendronate monohydrate (CaAL•H2 O)	416:458	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	35	theme	alendronate	424:434	arg1	O					457:457	CaAL•H2 O	449:457	CaAL•H2 O	449:457	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	36	theme	monohydrate	436:446	arg1	systems					405:411	delivery systems	396:411	delivery systems of calcium alendronate monohydrate (CaAL•H2 O)	396:458	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	36	theme	monohydrate	436:446	arg1	scaffolds					370:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	37	theme	glutaraldehyde	334:347	arg1	systems					405:411	delivery systems	396:411	delivery systems of calcium alendronate monohydrate (CaAL•H2 O)	396:458	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	37	theme	glutaraldehyde	334:347	arg1	scaffolds					370:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	38	theme	calcium	416:422	arg1	monohydrate					436:446	calcium alendronate monohydrate	416:446	calcium alendronate monohydrate (CaAL•H2 O)	416:458	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	38	theme	calcium	416:422	arg1	O					457:457	CaAL•H2 O	449:457	CaAL•H2 O	449:457	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	8	39	theme	markers	1096:1102	arg1	evaluation					1066:1075	The evaluation	1062:1075	The evaluation of differentiation markers	1062:1102	The evaluation of differentiation markers is performed both on the supernatants of cell culture and by means of quantitative polymerase chain reaction.
27748576	1	40	theme	alternative	283:293	arg1	modes					295:299	alternative modes	283:299	alternative modes of administration	283:317	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	0	41	theme	Delivery	28:35	arg1	Systems					37:43	Delivery Systems	28:43	Delivery Systems of Calcium Alendronate	28:66	Gelatin Porous Scaffolds as Delivery Systems of Calcium Alendronate.
27748576	1	42	theme	modes	295:299	arg1	implementation					265:278	implementation	265:278	implementation of alternative modes of administration	265:317	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	5	43	theme	BP	714:715	arg1	content					717:723	BP content	714:723	BP content	714:723	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	1	44	theme	several	211:217	arg1	effects					232:238	several adverse side effects	211:238	several adverse side effects	211:238	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	0	45	theme	Calcium	48:54	arg1	Alendronate					56:66	Calcium Alendronate	48:66	Calcium Alendronate	48:66	Gelatin Porous Scaffolds as Delivery Systems of Calcium Alendronate.
27748576	5	46	theme	collapse	811:818	arg1	strength					820:827	collapse strength	811:827	collapse strength	811:827	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	8	47	theme	differentiation	1080:1094	arg1	markers					1096:1102	differentiation markers	1080:1102	differentiation markers	1080:1102	The evaluation of differentiation markers is performed both on the supernatants of cell culture and by means of quantitative polymerase chain reaction.
27748576	3	48	theme	wt	593:594	arg1	%					595:595	0, 3, and 6 wt%	581:595	0, 3, and 6 wt%	581:595	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	3	48	theme	wt	593:594	arg1	content					572:578	CaAL•H2 O content	562:578	CaAL•H2 O content (0, 3, and 6 wt%)	562:596	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	3	49	theme	O	570:570	arg1	%					595:595	0, 3, and 6 wt%	581:595	0, 3, and 6 wt%	581:595	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	3	49	theme	O	570:570	arg1	content					572:578	CaAL•H2 O content	562:578	CaAL•H2 O content (0, 3, and 6 wt%)	562:596	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	9	50	theme	beneficial	1397:1406	arg1	role					1408:1411	a promising beneficial role	1385:1411	a promising beneficial role on bone repair processes	1385:1436	The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.
27748576	5	51	theme	relative	651:658	arg1	increase					660:667	The relative increase	647:667	The relative increase of the number of smaller pores on increasing BP content	647:723	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	1	52	theme	administration	304:317	arg1	modes					295:299	alternative modes	283:299	alternative modes of administration	283:317	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	3	53	theme	relevant	502:509	arg1	%					539:539	>94%	536:539	>94%	536:539	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	3	53	theme	relevant	502:509	arg1	porosity					526:533	a relevant interconnected porosity	500:533	a relevant interconnected porosity (>94%)	500:540	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	6	54	theme	CaAL•H2	864:870	arg1	profile					882:888	a sustained CaAL•H2 O release profile	852:888	a sustained CaAL•H2 O release profile	852:888	The scaffolds exhibit a sustained CaAL•H2 O release profile, and a significant amount of the drug is retained in the scaffolds even after 14 d.
27748576	4	55	theme	pore	612:615	arg1	size					617:620	pore size	612:620	pore size	612:620	At variance, pore size varies with composition.
27748576	8	56	theme	quantitative	1174:1185	arg1	reaction					1204:1211	quantitative polymerase chain reaction	1174:1211	quantitative polymerase chain reaction	1174:1211	The evaluation of differentiation markers is performed both on the supernatants of cell culture and by means of quantitative polymerase chain reaction.
27748576	9	57	theme	osteoblast	1272:1281	arg1	proliferation					1283:1295	osteoblast proliferation	1272:1295	osteoblast proliferation	1272:1295	The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.
27748576	1	58	theme	adverse	219:225	arg1	effects					232:238	several adverse side effects	211:238	several adverse side effects	211:238	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	8	59	theme	chain	1198:1202	arg1	reaction					1204:1211	quantitative polymerase chain reaction	1174:1211	quantitative polymerase chain reaction	1174:1211	The evaluation of differentiation markers is performed both on the supernatants of cell culture and by means of quantitative polymerase chain reaction.
27748576	3	60	theme	porous	475:480	arg1	scaffolds					482:490	The 3D highly porous scaffolds	461:490	The 3D highly porous scaffolds	461:490	The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H2 O content (0, 3, and 6 wt%).
27748576	9	61	theme	BP	1240:1241	arg1	scaffolds					1254:1262	BP containing scaffolds	1240:1262	BP containing scaffolds	1240:1262	The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.
27748576	1	62	theme	side	227:230	arg1	effects					232:238	several adverse side effects	211:238	several adverse side effects	211:238	The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration.
27748576	9	63	theme	promising	1387:1395	arg1	role					1408:1411	a promising beneficial role	1385:1411	a promising beneficial role on bone repair processes	1385:1436	The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.
27748576	8	64	theme	culture	1150:1156	arg1	supernatants					1129:1140	the supernatants	1125:1140	the supernatants of cell culture	1125:1156	The evaluation of differentiation markers is performed both on the supernatants of cell culture and by means of quantitative polymerase chain reaction.
27748576	5	65	theme	parallel	750:757	arg1	increase					771:778	the parallel significant increase	746:778	the parallel significant increase of the compressive modulus and collapse strength	746:827	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	2	66	theme	cross-linked	349:360	arg1	systems					405:411	delivery systems	396:411	delivery systems of calcium alendronate monohydrate (CaAL•H2 O)	396:458	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	66	theme	cross-linked	349:360	arg1	scaffolds					370:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	5	67	theme	smaller	686:692	arg1	pores					694:698	smaller pores	686:698	smaller pores on increasing BP content	686:723	The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength.
27748576	0	68	theme	Alendronate	56:66	arg1	Systems					37:43	Delivery Systems	28:43	Delivery Systems of Calcium Alendronate	28:66	Gelatin Porous Scaffolds as Delivery Systems of Calcium Alendronate.
27748576	6	69	theme	sustained	854:862	arg1	profile					882:888	a sustained CaAL•H2 O release profile	852:888	a sustained CaAL•H2 O release profile	852:888	The scaffolds exhibit a sustained CaAL•H2 O release profile, and a significant amount of the drug is retained in the scaffolds even after 14 d.
27748576	2	70	theme	gelatin	362:368	arg1	systems					405:411	delivery systems	396:411	delivery systems of calcium alendronate monohydrate (CaAL•H2 O)	396:458	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	2	70	theme	gelatin	362:368	arg1	scaffolds					370:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	glutaraldehyde cross-linked gelatin scaffolds	334:378	In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H2 O).
27748576	6	71	theme	release	874:880	arg1	profile					882:888	a sustained CaAL•H2 O release profile	852:888	a sustained CaAL•H2 O release profile	852:888	The scaffolds exhibit a sustained CaAL•H2 O release profile, and a significant amount of the drug is retained in the scaffolds even after 14 d.
26572331	6	0	theme	cell	945:948	arg1	current					950:956	100 mA cell current	938:956	100 mA cell current	938:956	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	5	1	theme	reinforced	728:737	arg1	membranes					744:752	textile reinforced agar membranes	720:752	textile reinforced agar membranes	720:752	In this study the potential electroosmosis on textile reinforced agar membranes as separation method was investigated.
26572331	9	2	from	transport	1275:1283	arg1	experiments					1390:1400	electroosmotic separation experiments	1364:1400	electroosmotic separation experiments	1364:1400	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	6	3	theme	100	938:940	arg1	mA					942:943	mA	942:943	mA	942:943	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	9	4	dep	ions	1288:1291	arg1	creatinine					1309:1318	creatinine	1309:1318	creatinine	1309:1318	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	9	4	dep	ions	1288:1291	arg1	PO4					1300:1302	PO4	1300:1302	PO4	1300:1302	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	9	4	dep	ions	1288:1291	arg1	K					1294:1294	K	1294:1294	K	1294:1294	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	5	5	theme	textile	720:726	arg1	membranes					744:752	textile reinforced agar membranes	720:752	textile reinforced agar membranes	720:752	In this study the potential electroosmosis on textile reinforced agar membranes as separation method was investigated.
26572331	6	6	theme	intensive	883:891	arg1	flow					908:911	an intensive electroosmotic flow	880:911	an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current	880:956	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	2	7	theme	physico-chemical	288:303	arg1	principle					305:313	the main physico-chemical principle	279:313	the main physico-chemical principle behind hemodialysis	279:333	Diffusion processes are the main physico-chemical principle behind hemodialysis.
26572331	2	7	theme	physico-chemical	288:303	arg1	processes					265:273	Diffusion processes	255:273	Diffusion processes	255:273	Diffusion processes are the main physico-chemical principle behind hemodialysis.
26572331	8	8	theme	molecular	1198:1206	arg1	molecules					1215:1223	uncharged low molecular weight molecules	1184:1223	uncharged low molecular weight molecules	1184:1223	Separation of uncharged low molecular weight molecules is determined by the membrane characteristic.
26572331	8	9	theme	molecules	1215:1223	arg1	Separation					1170:1179	Separation	1170:1179	Separation of uncharged low molecular weight molecules	1170:1223	Separation of uncharged low molecular weight molecules is determined by the membrane characteristic.
26572331	6	10	theme	membrane/cellulose	844:861	arg1	composite					870:878	an agar membrane/cellulose fabric composite	836:878	an agar membrane/cellulose fabric composite	836:878	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	4	11	dep	observed	634:641	arg1	consists					529:536	consists	529:536	consists of the non-ionic agarose and the negatively charged agaropectine	529:601	Agar consists of the non-ionic agarose and the negatively charged agaropectine thus an electroosmotic flux is observed in analytical electrophoresis.
26572331	10	12	theme	fluids	1618:1623	arg1	filtration					1593:1602	filtration	1593:1602	filtration of biological fluids in the absence of external pressure or high shear rates	1593:1679	The results demonstrate the potential of the method for filtration of biological fluids in the absence of external pressure or high shear rates.
26572331	1	13	theme	patients	219:226	arg1	%					211:211	70%	209:211	70% of US patients	209:226	Renal replacement therapy options are limited to hemodialysis and peritoneal dialysis (70% of US patients) or renal transplantation.
26572331	1	13	theme	patients	219:226	arg1	patients					219:226	US patients	216:226	US patients	216:226	Renal replacement therapy options are limited to hemodialysis and peritoneal dialysis (70% of US patients) or renal transplantation.
26572331	8	14	theme	uncharged	1184:1192	arg1	molecules					1215:1223	uncharged low molecular weight molecules	1184:1223	uncharged low molecular weight molecules	1184:1223	Separation of uncharged low molecular weight molecules is determined by the membrane characteristic.
26572331	6	15	dep	observed	967:974	arg1	Using					793:797	Using	793:797	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite	793:878	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	5	16	theme	agar	739:742	arg1	membranes					744:752	textile reinforced agar membranes	720:752	textile reinforced agar membranes	720:752	In this study the potential electroosmosis on textile reinforced agar membranes as separation method was investigated.
26572331	9	17	theme	ions	1288:1291	arg1	transport					1275:1283	The transport	1271:1283	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments	1271:1400	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	7	18	theme	agar	1027:1030	arg1	structure					1032:1040	the negatively charged agar structure	1004:1040	the negatively charged agar structure	1004:1040	The movement of cations in the negatively charged agar structure led to an intensive electroosmotic flux, which also transported uncharged molecules such as urea, glucose through the membrane.
26572331	1	19	theme	therapy	140:146	arg1	options					148:154	Renal replacement therapy options	122:154	Renal replacement therapy options	122:154	Renal replacement therapy options are limited to hemodialysis and peritoneal dialysis (70% of US patients) or renal transplantation.
26572331	7	20	theme	intensive	1052:1060	arg1	flux					1077:1080	an intensive electroosmotic flux	1049:1080	an intensive electroosmotic flux	1049:1080	The movement of cations in the negatively charged agar structure led to an intensive electroosmotic flux, which also transported uncharged molecules such as urea, glucose through the membrane.
26572331	9	21	theme	transport	1488:1496	arg1	processes					1498:1506	the overall transport processes	1476:1506	the overall transport processes in the electrochemical cell	1476:1534	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	3	22	theme	alternative	339:349	arg1	way					351:353	An alternative way	336:353	An alternative way	336:353	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	3	23	dep	bases	396:400	arg1	membranes					386:394	membranes	386:394	membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges	386:521	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	3	24	from	bases	396:400	arg1	flow					424:427	the electroosmotic flow	405:427	the electroosmotic flow	405:427	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	9	25	theme	electrochemical	1515:1529	arg1	cell					1531:1534	the electrochemical cell	1511:1534	the electrochemical cell	1511:1534	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	3	26	theme	liquid	366:371	arg1	flow					373:376	liquid flow	366:376	liquid flow	366:376	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	1	27	dep	hemodialysis	171:182	arg1	%					211:211	70%	209:211	70% of US patients	209:226	Renal replacement therapy options are limited to hemodialysis and peritoneal dialysis (70% of US patients) or renal transplantation.
26572331	1	27	dep	hemodialysis	171:182	arg1	patients					219:226	US patients	216:226	US patients	216:226	Renal replacement therapy options are limited to hemodialysis and peritoneal dialysis (70% of US patients) or renal transplantation.
26572331	0	28	theme	Multi-chamber	0:12	arg1	electroosmosis					14:27	Multi-chamber electroosmosis	0:27	Multi-chamber electroosmosis using textile	0:41	Multi-chamber electroosmosis using textile reinforced agar membranes--A promising concept for the future of hemodialysis.
26572331	4	29	theme	charged	582:588	arg1	agaropectine					590:601	the negatively charged agaropectine	567:601	the negatively charged agaropectine	567:601	Agar consists of the non-ionic agarose and the negatively charged agaropectine thus an electroosmotic flux is observed in analytical electrophoresis.
26572331	6	30	theme	h	929:929	arg1	flow					908:911	an intensive electroosmotic flow	880:911	an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current	880:956	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	1	31	theme	Renal	122:126	arg1	therapy					140:146	Renal replacement therapy	122:146	Renal replacement therapy options	122:154	Renal replacement therapy options are limited to hemodialysis and peritoneal dialysis (70% of US patients) or renal transplantation.
26572331	10	32	theme	external	1643:1650	arg1	pressure					1652:1659	external pressure	1643:1659	external pressure	1643:1659	The results demonstrate the potential of the method for filtration of biological fluids in the absence of external pressure or high shear rates.
26572331	3	33	theme	porous	479:484	arg1	membranes					486:494	porous membranes	479:494	porous membranes which bear surface charges	479:521	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	9	34	theme	molecules	1335:1343	arg1	transport					1275:1283	The transport	1271:1283	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments	1271:1400	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	0	35	theme	membranes--A	59:70	arg1	concept					82:88	agar membranes--A promising concept	54:88	agar membranes--A promising concept for the future of hemodialysis	54:119	Multi-chamber electroosmosis using textile reinforced agar membranes--A promising concept for the future of hemodialysis.
26572331	6	36	theme	cm	923:924	arg1	-1					931:932	-1	931:932	-1	931:932	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	6	36	theme	cm	923:924	arg1	h					929:929	1-2 ml cm(2) h	916:929	1-2 ml cm(2) h(-1)	916:933	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	6	37	theme	electrolysis	814:825	arg1	cell					827:830	a five-chamber electrolysis cell	799:830	a five-chamber electrolysis cell	799:830	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	3	38	theme	surface	507:513	arg1	charges					515:521	surface charges	507:521	surface charges	507:521	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	1	39	theme	renal	232:236	arg1	transplantation					238:252	renal transplantation	232:252	renal transplantation	232:252	Renal replacement therapy options are limited to hemodialysis and peritoneal dialysis (70% of US patients) or renal transplantation.
26572331	6	40	from	current	950:956	arg1	flow					908:911	an intensive electroosmotic flow	880:911	an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current	880:956	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	5	41	theme	potential	692:700	arg1	electroosmosis					702:715	the potential electroosmosis	688:715	the potential electroosmosis on textile reinforced agar membranes as separation method	688:773	In this study the potential electroosmosis on textile reinforced agar membranes as separation method was investigated.
26572331	9	42	theme	separation	1379:1388	arg1	experiments					1390:1400	electroosmotic separation experiments	1364:1400	electroosmotic separation experiments	1364:1400	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	6	43	theme	mA	942:943	arg1	current					950:956	100 mA cell current	938:956	100 mA cell current	938:956	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	9	44	from	processes	1498:1506	arg1	cell					1531:1534	the electrochemical cell	1511:1534	the electrochemical cell	1511:1534	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	4	45	theme	electroosmotic	611:624	arg1	flux					626:629	an electroosmotic flux	608:629	an electroosmotic flux	608:629	Agar consists of the non-ionic agarose and the negatively charged agaropectine thus an electroosmotic flux is observed in analytical electrophoresis.
26572331	6	46	theme	electroosmotic	893:906	arg1	flow					908:911	an intensive electroosmotic flow	880:911	an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current	880:956	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	10	47	from	filtration	1593:1602	arg1	absence					1632:1638	the absence	1628:1638	the absence of external pressure or high shear rates	1628:1679	The results demonstrate the potential of the method for filtration of biological fluids in the absence of external pressure or high shear rates.
26572331	2	48	theme	main	283:286	arg1	principle					305:313	the main physico-chemical principle	279:313	the main physico-chemical principle behind hemodialysis	279:333	Diffusion processes are the main physico-chemical principle behind hemodialysis.
26572331	2	48	theme	main	283:286	arg1	processes					265:273	Diffusion processes	255:273	Diffusion processes	255:273	Diffusion processes are the main physico-chemical principle behind hemodialysis.
26572331	1	49	theme	peritoneal	188:197	arg1	dialysis					199:206	peritoneal dialysis	188:206	peritoneal dialysis	188:206	Renal replacement therapy options are limited to hemodialysis and peritoneal dialysis (70% of US patients) or renal transplantation.
26572331	8	50	theme	low	1194:1196	arg1	molecules					1215:1223	uncharged low molecular weight molecules	1184:1223	uncharged low molecular weight molecules	1184:1223	Separation of uncharged low molecular weight molecules is determined by the membrane characteristic.
26572331	10	51	theme	method	1582:1587	arg1	potential					1565:1573	the potential	1561:1573	the potential of the method for filtration of biological fluids in the absence of external pressure or high shear rates	1561:1679	The results demonstrate the potential of the method for filtration of biological fluids in the absence of external pressure or high shear rates.
26572331	7	52	theme	uncharged	1106:1114	arg1	molecules					1116:1124	uncharged molecules	1106:1124	uncharged molecules such as urea, glucose	1106:1146	The movement of cations in the negatively charged agar structure led to an intensive electroosmotic flux, which also transported uncharged molecules such as urea, glucose through the membrane.
26572331	7	52	theme	uncharged	1106:1114	arg1	urea					1134:1137	urea	1134:1137	urea	1134:1137	The movement of cations in the negatively charged agar structure led to an intensive electroosmotic flux, which also transported uncharged molecules such as urea, glucose through the membrane.
26572331	6	53	theme	fabric	863:868	arg1	composite					870:878	an agar membrane/cellulose fabric composite	836:878	an agar membrane/cellulose fabric composite	836:878	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	9	54	theme	pH	1424:1425	arg1	electrolyte					1441:1451	a pH 5.5 phosphate electrolyte	1422:1451	a pH 5.5 phosphate electrolyte	1422:1451	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	8	55	theme	weight	1208:1213	arg1	molecules					1215:1223	uncharged low molecular weight molecules	1184:1223	uncharged low molecular weight molecules	1184:1223	Separation of uncharged low molecular weight molecules is determined by the membrane characteristic.
26572331	5	56	theme	separation	757:766	arg1	method					768:773	separation method	757:773	separation method	757:773	In this study the potential electroosmosis on textile reinforced agar membranes as separation method was investigated.
26572331	6	57	theme	agar	839:842	arg1	composite					870:878	an agar membrane/cellulose fabric composite	836:878	an agar membrane/cellulose fabric composite	836:878	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	9	58	theme	phosphate	1431:1439	arg1	electrolyte					1441:1451	a pH 5.5 phosphate electrolyte	1422:1451	a pH 5.5 phosphate electrolyte	1422:1451	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	10	59	theme	biological	1607:1616	arg1	fluids					1618:1623	biological fluids	1607:1623	biological fluids	1607:1623	The results demonstrate the potential of the method for filtration of biological fluids in the absence of external pressure or high shear rates.
26572331	1	60	theme	US	216:217	arg1	patients					219:226	US patients	216:226	US patients	216:226	Renal replacement therapy options are limited to hemodialysis and peritoneal dialysis (70% of US patients) or renal transplantation.
26572331	3	61	contain	bear	502:505	arg1	membranes					486:494	porous membranes	479:494	porous membranes which bear surface charges	479:521	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	3	61	contain	bear	502:505	arg2	charges					515:521	surface charges	507:521	surface charges	507:521	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	7	62	theme	electroosmotic	1062:1075	arg1	flux					1077:1080	an intensive electroosmotic flux	1049:1080	an intensive electroosmotic flux	1049:1080	The movement of cations in the negatively charged agar structure led to an intensive electroosmotic flux, which also transported uncharged molecules such as urea, glucose through the membrane.
26572331	1	63	theme	replacement	128:138	arg1	therapy					140:146	Renal replacement therapy	122:146	Renal replacement therapy options	122:154	Renal replacement therapy options are limited to hemodialysis and peritoneal dialysis (70% of US patients) or renal transplantation.
26572331	8	64	theme	membrane	1246:1253	arg1	characteristic					1255:1268	the membrane characteristic	1242:1268	the membrane characteristic	1242:1268	Separation of uncharged low molecular weight molecules is determined by the membrane characteristic.
26572331	10	65	theme	shear	1669:1673	arg1	rates					1675:1679	high shear rates	1664:1679	high shear rates	1664:1679	The results demonstrate the potential of the method for filtration of biological fluids in the absence of external pressure or high shear rates.
26572331	2	66	theme	Diffusion	255:263	arg1	principle					305:313	the main physico-chemical principle	279:313	the main physico-chemical principle behind hemodialysis	279:333	Diffusion processes are the main physico-chemical principle behind hemodialysis.
26572331	2	66	theme	Diffusion	255:263	arg1	processes					265:273	Diffusion processes	255:273	Diffusion processes	255:273	Diffusion processes are the main physico-chemical principle behind hemodialysis.
26572331	7	67	theme	charged	1019:1025	arg1	structure					1032:1040	the negatively charged agar structure	1004:1040	the negatively charged agar structure	1004:1040	The movement of cations in the negatively charged agar structure led to an intensive electroosmotic flux, which also transported uncharged molecules such as urea, glucose through the membrane.
26572331	9	68	theme	overall	1480:1486	arg1	processes					1498:1506	the overall transport processes	1476:1506	the overall transport processes in the electrochemical cell	1476:1534	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	7	69	from	movement	981:988	arg1	structure					1032:1040	the negatively charged agar structure	1004:1040	the negatively charged agar structure	1004:1040	The movement of cations in the negatively charged agar structure led to an intensive electroosmotic flux, which also transported uncharged molecules such as urea, glucose through the membrane.
26572331	4	70	theme	non-ionic	545:553	arg1	agarose					555:561	the non-ionic agarose	541:561	the non-ionic agarose	541:561	Agar consists of the non-ionic agarose and the negatively charged agaropectine thus an electroosmotic flux is observed in analytical electrophoresis.
26572331	0	71	theme	hemodialysis	108:119	arg1	future					98:103	future	98:103	future	98:103	Multi-chamber electroosmosis using textile reinforced agar membranes--A promising concept for the future of hemodialysis.
26572331	7	72	theme	cations	993:999	arg1	movement					981:988	The movement	977:988	The movement of cations in the negatively charged agar structure	977:1040	The movement of cations in the negatively charged agar structure led to an intensive electroosmotic flux, which also transported uncharged molecules such as urea, glucose through the membrane.
26572331	9	73	dep	molecules	1335:1343	arg1	glucose					1352:1358	glucose	1352:1358	glucose	1352:1358	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	9	73	dep	molecules	1335:1343	arg1	urea					1346:1349	urea	1346:1349	urea	1346:1349	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	3	74	theme	electroosmotic	409:422	arg1	flow					424:427	the electroosmotic flow	405:427	the electroosmotic flow	405:427	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	3	75	theme	electrokinetic	450:463	arg1	phenomenon					465:474	electrokinetic phenomenon	450:474	electrokinetic phenomenon in porous membranes which bear surface charges	450:521	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	3	75	theme	electrokinetic	450:463	arg1	bases					396:400	bases	396:400	bases on the electroosmotic flow	396:427	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	10	76	theme	pressure	1652:1659	arg1	absence					1632:1638	the absence	1628:1638	the absence of external pressure or high shear rates	1628:1679	The results demonstrate the potential of the method for filtration of biological fluids in the absence of external pressure or high shear rates.
26572331	0	77	theme	agar	54:57	arg1	concept					82:88	agar membranes--A promising concept	54:88	agar membranes--A promising concept for the future of hemodialysis	54:119	Multi-chamber electroosmosis using textile reinforced agar membranes--A promising concept for the future of hemodialysis.
26572331	9	78	theme	uncharged	1325:1333	arg1	molecules					1335:1343	uncharged molecules	1325:1343	uncharged molecules (urea, glucose)	1325:1359	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
26572331	0	79	theme	promising	72:80	arg1	concept					82:88	agar membranes--A promising concept	54:88	agar membranes--A promising concept for the future of hemodialysis	54:119	Multi-chamber electroosmosis using textile reinforced agar membranes--A promising concept for the future of hemodialysis.
26572331	10	80	theme	rates	1675:1679	arg1	absence					1632:1638	the absence	1628:1638	the absence of external pressure or high shear rates	1628:1679	The results demonstrate the potential of the method for filtration of biological fluids in the absence of external pressure or high shear rates.
26572331	5	81	from	electroosmosis	702:715	arg1	membranes					744:752	textile reinforced agar membranes	720:752	textile reinforced agar membranes	720:752	In this study the potential electroosmosis on textile reinforced agar membranes as separation method was investigated.
26572331	6	82	theme	ml	920:921	arg1	-1					931:932	-1	931:932	-1	931:932	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	6	82	theme	ml	920:921	arg1	h					929:929	1-2 ml cm(2) h	916:929	1-2 ml cm(2) h(-1)	916:933	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	4	83	theme	analytical	646:655	arg1	electrophoresis					657:671	analytical electrophoresis	646:671	analytical electrophoresis	646:671	Agar consists of the non-ionic agarose and the negatively charged agaropectine thus an electroosmotic flux is observed in analytical electrophoresis.
26572331	6	84	theme	five-chamber	801:812	arg1	cell					827:830	a five-chamber electrolysis cell	799:830	a five-chamber electrolysis cell	799:830	Using a five-chamber electrolysis cell and an agar membrane/cellulose fabric composite an intensive electroosmotic flow of 1-2 ml cm(2) h(-1) at 100 mA cell current could be observed.
26572331	10	85	theme	high	1664:1667	arg1	rates					1675:1679	high shear rates	1664:1679	high shear rates	1664:1679	The results demonstrate the potential of the method for filtration of biological fluids in the absence of external pressure or high shear rates.
26572331	4	86	located	observed	634:641	arg1	electrophoresis					657:671	analytical electrophoresis	646:671	analytical electrophoresis	646:671	Agar consists of the non-ionic agarose and the negatively charged agaropectine thus an electroosmotic flux is observed in analytical electrophoresis.
26572331	4	86	located	observed	634:641	arg2	flux					626:629	an electroosmotic flux	608:629	an electroosmotic flux	608:629	Agar consists of the non-ionic agarose and the negatively charged agaropectine thus an electroosmotic flux is observed in analytical electrophoresis.
26572331	3	87	from	phenomenon	465:474	arg1	membranes					486:494	porous membranes	479:494	porous membranes which bear surface charges	479:521	An alternative way to achieve liquid flow through membranes bases on the electroosmotic flow which is observed as electrokinetic phenomenon in porous membranes which bear surface charges.
26572331	9	88	theme	electroosmotic	1364:1377	arg1	experiments					1390:1400	electroosmotic separation experiments	1364:1400	electroosmotic separation experiments	1364:1400	The transport of ions (K(+), PO4(3-), creatinine) and uncharged molecules (urea, glucose) in electroosmotic separation experiments was monitored using a pH 5.5 phosphate electrolyte with the aim to assess the overall transport processes in the electrochemical cell.
27497286	0	0	theme	pectin	87:92	arg1	modification					94:105	pectin modification	87:105	pectin modification	87:105	Rice putative methyltransferase gene OsTSD2 is required for root development involving pectin modification.
27497286	6	1	theme	acid	990:993	arg1	content					995:1001	the uronic acid content	979:1001	the uronic acid content	979:1001	Biochemical analysis of cell wall pectin polysaccharides revealed that both the monosaccharide composition and the uronic acid content were decreased in Ostsd2 mutants.
27497286	8	2	theme	root	1378:1381	arg1	development					1383:1393	root development	1378:1393	root development	1378:1393	A hypothesis to explain the relationship among OsTSD2, pectin methylesterification, and root development is proposed, based on pectin's function in regional cell extension/division in a zone-dependent manner.
27497286	5	3	theme	roots	782:786	arg1	staining					766:773	Immunofluorescence (with LM19) and Ruthenium Red staining	717:773	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots	717:786	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots showed that unesterified homogalacturonan (HG) was increased in Ostsd2 mutants.
27497286	4	4	theme	cellular	533:540	arg1	adhesion					542:549	abnormal cellular adhesion	524:549	abnormal cellular adhesion	524:549	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	7	5	theme	Ostsd2	1153:1158	arg1	mutants					1160:1166	the Ostsd2 mutants	1149:1166	the Ostsd2 mutants	1149:1166	Increased endogenous ABA content and opposite roles performed by ABA and IAA in regulating cellular adhesion in the Ostsd2 mutants suggested that OsTSD2 is required for root development in rice through a pathway involving pectin synthesis/modification.
27497286	6	6	theme	Ostsd2	1021:1026	arg1	mutants					1028:1034	Ostsd2 mutants	1021:1034	Ostsd2 mutants	1021:1034	Biochemical analysis of cell wall pectin polysaccharides revealed that both the monosaccharide composition and the uronic acid content were decreased in Ostsd2 mutants.
27497286	4	7	theme	abnormal	524:531	arg1	adhesion					542:549	abnormal cellular adhesion	524:549	abnormal cellular adhesion	524:549	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	2	8	theme	OsTSD2	296:301	arg1	methyltransferase					334:350	a putative methyltransferase	323:350	a putative methyltransferase in rice	323:358	Here, we report the functional characterization of the OsTSD2 gene, which encodes a putative methyltransferase in rice.
27497286	2	8	theme	OsTSD2	296:301	arg1	gene					303:306	the OsTSD2 gene	292:306	the OsTSD2 gene	292:306	Here, we report the functional characterization of the OsTSD2 gene, which encodes a putative methyltransferase in rice.
27497286	4	9	theme	root	684:687	arg1	density					689:695	lateral root density	676:695	lateral root density in the mature zone	676:714	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	4	10	theme	root	625:628	arg1	elongation					630:639	root elongation	625:639	root elongation	625:639	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	6	11	theme	polysaccharides	909:923	arg1	analysis					880:887	Biochemical analysis	868:887	Biochemical analysis of cell wall pectin polysaccharides	868:923	Biochemical analysis of cell wall pectin polysaccharides revealed that both the monosaccharide composition and the uronic acid content were decreased in Ostsd2 mutants.
27497286	5	12	theme	Ruthenium	752:760	arg1	staining					766:773	Immunofluorescence (with LM19) and Ruthenium Red staining	717:773	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots	717:786	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots showed that unesterified homogalacturonan (HG) was increased in Ostsd2 mutants.
27497286	6	13	theme	pectin	902:907	arg1	polysaccharides					909:923	cell wall pectin polysaccharides	892:923	cell wall pectin polysaccharides	892:923	Biochemical analysis of cell wall pectin polysaccharides revealed that both the monosaccharide composition and the uronic acid content were decreased in Ostsd2 mutants.
27497286	2	14	from	methyltransferase	334:350	arg1	rice					355:358	rice	355:358	rice	355:358	Here, we report the functional characterization of the OsTSD2 gene, which encodes a putative methyltransferase in rice.
27497286	4	15	from	density	689:695	arg1	zone					711:714	the mature zone	700:714	the mature zone	700:714	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	3	16	theme	serial	446:451	arg1	alterations					453:463	serial alterations	446:463	serial alterations	446:463	All three independent T-DNA insertion lines of OsTSD2 displayed dwarf phenotypes and serial alterations in different zones of the root.
27497286	5	17	theme	Red	762:764	arg1	Ruthenium					752:760	Ruthenium Red	752:764	Ruthenium Red	752:764	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots showed that unesterified homogalacturonan (HG) was increased in Ostsd2 mutants.
27497286	6	18	theme	wall	897:900	arg1	polysaccharides					909:923	cell wall pectin polysaccharides	892:923	cell wall pectin polysaccharides	892:923	Biochemical analysis of cell wall pectin polysaccharides revealed that both the monosaccharide composition and the uronic acid content were decreased in Ostsd2 mutants.
27497286	7	19	theme	cellular	1128:1135	arg1	adhesion					1137:1144	cellular adhesion	1128:1144	cellular adhesion	1128:1144	Increased endogenous ABA content and opposite roles performed by ABA and IAA in regulating cellular adhesion in the Ostsd2 mutants suggested that OsTSD2 is required for root development in rice through a pathway involving pectin synthesis/modification.
27497286	7	20	theme	root	1206:1209	arg1	development					1211:1221	root development	1206:1221	root development in rice through a pathway involving pectin synthesis/modification	1206:1287	Increased endogenous ABA content and opposite roles performed by ABA and IAA in regulating cellular adhesion in the Ostsd2 mutants suggested that OsTSD2 is required for root development in rice through a pathway involving pectin synthesis/modification.
27497286	4	21	theme	lateral	676:682	arg1	density					689:695	lateral root density	676:695	lateral root density in the mature zone	676:714	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	0	22	theme	putative	5:12	arg1	OsTSD2					37:42	Rice putative methyltransferase gene OsTSD2	0:42	Rice putative methyltransferase gene OsTSD2	0:42	Rice putative methyltransferase gene OsTSD2 is required for root development involving pectin modification.
27497286	6	23	theme	cell	892:895	arg1	polysaccharides					909:923	cell wall pectin polysaccharides	892:923	cell wall pectin polysaccharides	892:923	Biochemical analysis of cell wall pectin polysaccharides revealed that both the monosaccharide composition and the uronic acid content were decreased in Ostsd2 mutants.
27497286	8	24	dep	hypothesis	1292:1301	arg1	explain					1306:1312	explain	1306:1312	to explain the relationship among OsTSD2, pectin methylesterification, and root development	1303:1393	A hypothesis to explain the relationship among OsTSD2, pectin methylesterification, and root development is proposed, based on pectin's function in regional cell extension/division in a zone-dependent manner.
27497286	8	25	from	function	1426:1433	arg1	extension/division					1452:1469	regional cell extension/division	1438:1469	regional cell extension/division in a zone-dependent manner	1438:1496	A hypothesis to explain the relationship among OsTSD2, pectin methylesterification, and root development is proposed, based on pectin's function in regional cell extension/division in a zone-dependent manner.
27497286	1	26	theme	underlying	188:197	arg1	mechanisms					199:208	the underlying mechanisms	184:208	the underlying mechanisms	184:208	Pectin synthesis and modification are vital for plant development, although the underlying mechanisms are still not well understood.
27497286	0	27	theme	Rice	0:3	arg1	OsTSD2					37:42	Rice putative methyltransferase gene OsTSD2	0:42	Rice putative methyltransferase gene OsTSD2	0:42	Rice putative methyltransferase gene OsTSD2 is required for root development involving pectin modification.
27497286	6	28	theme	monosaccharide	948:961	arg1	composition					963:973	the monosaccharide composition	944:973	the monosaccharide composition	944:973	Biochemical analysis of cell wall pectin polysaccharides revealed that both the monosaccharide composition and the uronic acid content were decreased in Ostsd2 mutants.
27497286	3	29	theme	independent	371:381	arg1	lines					399:403	All three independent T-DNA insertion lines	361:403	All three independent T-DNA insertion lines of OsTSD2	361:413	All three independent T-DNA insertion lines of OsTSD2 displayed dwarf phenotypes and serial alterations in different zones of the root.
27497286	5	30	theme	Immunofluorescence	717:734	arg1	staining					766:773	Immunofluorescence (with LM19) and Ruthenium Red staining	717:773	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots	717:786	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots showed that unesterified homogalacturonan (HG) was increased in Ostsd2 mutants.
27497286	8	31	theme	regional	1438:1445	arg1	extension/division					1452:1469	regional cell extension/division	1438:1469	regional cell extension/division in a zone-dependent manner	1438:1496	A hypothesis to explain the relationship among OsTSD2, pectin methylesterification, and root development is proposed, based on pectin's function in regional cell extension/division in a zone-dependent manner.
27497286	1	32	theme	Pectin	108:113	arg1	synthesis					115:123	Pectin synthesis	108:123	Pectin synthesis	108:123	Pectin synthesis and modification are vital for plant development, although the underlying mechanisms are still not well understood.
27497286	3	33	theme	T-DNA	383:387	arg1	lines					399:403	All three independent T-DNA insertion lines	361:403	All three independent T-DNA insertion lines of OsTSD2	361:413	All three independent T-DNA insertion lines of OsTSD2 displayed dwarf phenotypes and serial alterations in different zones of the root.
27497286	4	34	theme	meristematic	596:607	arg1	zone					609:612	the meristematic zone	592:612	the meristematic zone	592:612	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	7	35	from	development	1211:1221	arg1	rice					1226:1229	rice	1226:1229	rice	1226:1229	Increased endogenous ABA content and opposite roles performed by ABA and IAA in regulating cellular adhesion in the Ostsd2 mutants suggested that OsTSD2 is required for root development in rice through a pathway involving pectin synthesis/modification.
27497286	2	36	theme	gene	303:306	arg1	characterization					272:287	the functional characterization	257:287	the functional characterization of the OsTSD2 gene, which encodes a putative methyltransferase in rice	257:358	Here, we report the functional characterization of the OsTSD2 gene, which encodes a putative methyltransferase in rice.
27497286	6	37	theme	uronic	983:988	arg1	content					995:1001	the uronic acid content	979:1001	the uronic acid content	979:1001	Biochemical analysis of cell wall pectin polysaccharides revealed that both the monosaccharide composition and the uronic acid content were decreased in Ostsd2 mutants.
27497286	5	38	with	Immunofluorescence	717:734	arg1	LM19					742:745	LM19	742:745	LM19	742:745	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots showed that unesterified homogalacturonan (HG) was increased in Ostsd2 mutants.
27497286	5	39	with	Ruthenium	752:760	arg1	LM19					742:745	LM19	742:745	LM19	742:745	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots showed that unesterified homogalacturonan (HG) was increased in Ostsd2 mutants.
27497286	8	40	from	extension/division	1452:1469	arg1	manner					1491:1496	a zone-dependent manner	1474:1496	a zone-dependent manner	1474:1496	A hypothesis to explain the relationship among OsTSD2, pectin methylesterification, and root development is proposed, based on pectin's function in regional cell extension/division in a zone-dependent manner.
27497286	5	41	theme	Ostsd2	852:857	arg1	mutants					859:865	Ostsd2 mutants	852:865	Ostsd2 mutants	852:865	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots showed that unesterified homogalacturonan (HG) was increased in Ostsd2 mutants.
27497286	3	42	theme	dwarf	425:429	arg1	phenotypes					431:440	dwarf phenotypes	425:440	dwarf phenotypes	425:440	All three independent T-DNA insertion lines of OsTSD2 displayed dwarf phenotypes and serial alterations in different zones of the root.
27497286	6	43	theme	Biochemical	868:878	arg1	analysis					880:887	Biochemical analysis	868:887	Biochemical analysis of cell wall pectin polysaccharides	868:923	Biochemical analysis of cell wall pectin polysaccharides revealed that both the monosaccharide composition and the uronic acid content were decreased in Ostsd2 mutants.
27497286	0	44	theme	gene	32:35	arg1	OsTSD2					37:42	Rice putative methyltransferase gene OsTSD2	0:42	Rice putative methyltransferase gene OsTSD2	0:42	Rice putative methyltransferase gene OsTSD2 is required for root development involving pectin modification.
27497286	7	45	theme	endogenous	1047:1056	arg1	content					1062:1068	Increased endogenous ABA content	1037:1068	Increased endogenous ABA content	1037:1068	Increased endogenous ABA content and opposite roles performed by ABA and IAA in regulating cellular adhesion in the Ostsd2 mutants suggested that OsTSD2 is required for root development in rice through a pathway involving pectin synthesis/modification.
27497286	3	46	theme	different	468:476	arg1	zones					478:482	different zones	468:482	different zones of the root	468:494	All three independent T-DNA insertion lines of OsTSD2 displayed dwarf phenotypes and serial alterations in different zones of the root.
27497286	5	47	theme	unesterified	800:811	arg1	HG					831:832	HG	831:832	HG	831:832	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots showed that unesterified homogalacturonan (HG) was increased in Ostsd2 mutants.
27497286	5	47	theme	unesterified	800:811	arg1	homogalacturonan					813:828	unesterified homogalacturonan	800:828	unesterified homogalacturonan (HG)	800:833	Immunofluorescence (with LM19) and Ruthenium Red staining of the roots showed that unesterified homogalacturonan (HG) was increased in Ostsd2 mutants.
27497286	7	48	theme	opposite	1074:1081	arg1	roles					1083:1087	opposite roles	1074:1087	opposite roles	1074:1087	Increased endogenous ABA content and opposite roles performed by ABA and IAA in regulating cellular adhesion in the Ostsd2 mutants suggested that OsTSD2 is required for root development in rice through a pathway involving pectin synthesis/modification.
27497286	7	49	theme	pectin	1259:1264	arg1	synthesis/modification					1266:1287	pectin synthesis/modification	1259:1287	pectin synthesis/modification	1259:1287	Increased endogenous ABA content and opposite roles performed by ABA and IAA in regulating cellular adhesion in the Ostsd2 mutants suggested that OsTSD2 is required for root development in rice through a pathway involving pectin synthesis/modification.
27497286	8	50	theme	cell	1447:1450	arg1	extension/division					1452:1469	regional cell extension/division	1438:1469	regional cell extension/division in a zone-dependent manner	1438:1496	A hypothesis to explain the relationship among OsTSD2, pectin methylesterification, and root development is proposed, based on pectin's function in regional cell extension/division in a zone-dependent manner.
27497286	7	51	theme	ABA	1058:1060	arg1	content					1062:1068	Increased endogenous ABA content	1037:1068	Increased endogenous ABA content	1037:1068	Increased endogenous ABA content and opposite roles performed by ABA and IAA in regulating cellular adhesion in the Ostsd2 mutants suggested that OsTSD2 is required for root development in rice through a pathway involving pectin synthesis/modification.
27497286	3	52	theme	OsTSD2	408:413	arg1	lines					399:403	All three independent T-DNA insertion lines	361:403	All three independent T-DNA insertion lines of OsTSD2	361:413	All three independent T-DNA insertion lines of OsTSD2 displayed dwarf phenotypes and serial alterations in different zones of the root.
27497286	4	53	theme	aerenchyma	568:577	arg1	formation					579:587	schizogenous aerenchyma formation	555:587	schizogenous aerenchyma formation	555:587	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	1	54	theme	plant	156:160	arg1	development					162:172	plant development	156:172	plant development	156:172	Pectin synthesis and modification are vital for plant development, although the underlying mechanisms are still not well understood.
27497286	4	55	theme	schizogenous	555:566	arg1	formation					579:587	schizogenous aerenchyma formation	555:587	schizogenous aerenchyma formation	555:587	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	2	56	theme	functional	261:270	arg1	characterization					272:287	the functional characterization	257:287	the functional characterization of the OsTSD2 gene, which encodes a putative methyltransferase in rice	257:358	Here, we report the functional characterization of the OsTSD2 gene, which encodes a putative methyltransferase in rice.
27497286	7	57	theme	Increased	1037:1045	arg1	content					1062:1068	Increased endogenous ABA content	1037:1068	Increased endogenous ABA content	1037:1068	Increased endogenous ABA content and opposite roles performed by ABA and IAA in regulating cellular adhesion in the Ostsd2 mutants suggested that OsTSD2 is required for root development in rice through a pathway involving pectin synthesis/modification.
27497286	2	58	theme	putative	325:332	arg1	methyltransferase					334:350	a putative methyltransferase	323:350	a putative methyltransferase in rice	323:358	Here, we report the functional characterization of the OsTSD2 gene, which encodes a putative methyltransferase in rice.
27497286	2	58	theme	putative	325:332	arg1	gene					303:306	the OsTSD2 gene	292:306	the OsTSD2 gene	292:306	Here, we report the functional characterization of the OsTSD2 gene, which encodes a putative methyltransferase in rice.
27497286	4	59	dep	density	689:695	arg1	higher					669:674	higher	669:674	higher	669:674	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	3	60	theme	root	491:494	arg1	zones					478:482	different zones	468:482	different zones of the root	468:494	All three independent T-DNA insertion lines of OsTSD2 displayed dwarf phenotypes and serial alterations in different zones of the root.
27497286	8	61	theme	pectin	1345:1350	arg1	methylesterification					1352:1371	pectin methylesterification	1345:1371	pectin methylesterification	1345:1371	A hypothesis to explain the relationship among OsTSD2, pectin methylesterification, and root development is proposed, based on pectin's function in regional cell extension/division in a zone-dependent manner.
27497286	8	62	theme	zone-dependent	1476:1489	arg1	manner					1491:1496	a zone-dependent manner	1474:1496	a zone-dependent manner	1474:1496	A hypothesis to explain the relationship among OsTSD2, pectin methylesterification, and root development is proposed, based on pectin's function in regional cell extension/division in a zone-dependent manner.
27497286	4	63	theme	mature	704:709	arg1	zone					711:714	the mature zone	700:714	the mature zone	700:714	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	4	64	theme	elongation	648:657	arg1	zone					659:662	the elongation zone	644:662	the elongation zone	644:662	These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone.
27497286	0	65	theme	root	60:63	arg1	development					65:75	root development	60:75	root development involving pectin modification	60:105	Rice putative methyltransferase gene OsTSD2 is required for root development involving pectin modification.
27497286	0	66	theme	methyltransferase	14:30	arg1	OsTSD2					37:42	Rice putative methyltransferase gene OsTSD2	0:42	Rice putative methyltransferase gene OsTSD2	0:42	Rice putative methyltransferase gene OsTSD2 is required for root development involving pectin modification.
27497286	3	67	theme	insertion	389:397	arg1	lines					399:403	All three independent T-DNA insertion lines	361:403	All three independent T-DNA insertion lines of OsTSD2	361:413	All three independent T-DNA insertion lines of OsTSD2 displayed dwarf phenotypes and serial alterations in different zones of the root.
24478313	0	0	theme	secretory	91:99	arg1	modification					20:31	Proteolytic histone modification	0:31	Proteolytic histone modification by mast cell tryptase	0:53	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	0	0	theme	secretory	91:99	arg1	protease					109:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	5	1	theme	cytotoxic	1125:1133	arg1	agent					1135:1139	cytotoxic agent	1125:1139	cytotoxic agent	1125:1139	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	8	2	theme	granule-derived	1522:1536	arg1	axis					1557:1560	a secretory granule-derived serglycin-tryptase axis	1510:1560	a secretory granule-derived serglycin-tryptase axis	1510:1560	Together, these findings implicate histone proteolysis through a secretory granule-derived serglycin-tryptase axis as a novel principle for histone modification, during both cell homeostasis and cell death.
24478313	4	3	theme	cell	729:732	arg1	process					740:746	the cell death process	725:746	the cell death process	725:746	During the cell death process, tryptase was found to relocalize from granules into the cytosol and nucleus, and it was found that the absence of tryptase was associated with a pronounced accumulation of core histones both in the cytosol and in the nucleus.
24478313	5	4	contain	has	1167:1169	arg1	tryptase					1158:1165	tryptase	1158:1165	tryptase	1158:1165	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	5	4	contain	has	1167:1169	arg2	impact					1185:1190	a homeostatic impact	1171:1190	a homeostatic impact on nuclear events	1171:1208	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	5	5	theme	nuclear	1195:1201	arg1	events					1203:1208	nuclear events	1195:1208	nuclear events	1195:1208	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	0	6	theme	proteoglycan-dependent	68:89	arg1	modification					20:31	Proteolytic histone modification	0:31	Proteolytic histone modification by mast cell tryptase	0:53	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	0	6	theme	proteoglycan-dependent	68:89	arg1	protease					109:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	5	7	theme	agent	1135:1139	arg1	absence					1114:1120	the absence	1110:1120	the absence of cytotoxic agent	1110:1139	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	4	8	theme	tryptase	863:870	arg1	absence					852:858	the absence	848:858	the absence of tryptase	848:870	During the cell death process, tryptase was found to relocalize from granules into the cytosol and nucleus, and it was found that the absence of tryptase was associated with a pronounced accumulation of core histones both in the cytosol and in the nucleus.
24478313	1	9	theme	high	161:164	arg1	content					166:172	their high content	155:172	their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	155:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	1	9	theme	high	161:164	arg1	feature					130:136	A hallmark feature	119:136	A hallmark feature of mast cells	119:150	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	5	10	dep	even	1073:1076	arg1	i.e.					1102:1105	i.e.	1102:1105	i.e.	1102:1105	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	1	11	theme	serglycin	358:366	arg1	proteoglycan					368:379	serglycin proteoglycan	358:379	serglycin proteoglycan	358:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	6	12	theme	cells	1263:1267	arg1	nucleus					1245:1251	the nucleus	1241:1251	the nucleus of viable cells	1241:1267	Indeed, tryptase was found in the nucleus of viable cells and was shown to cleave core histones in their N-terminal tail.
24478313	8	13	theme	cell	1621:1624	arg1	homeostasis					1626:1636	cell homeostasis	1621:1636	cell homeostasis	1621:1636	Together, these findings implicate histone proteolysis through a secretory granule-derived serglycin-tryptase axis as a novel principle for histone modification, during both cell homeostasis and cell death.
24478313	1	14	theme	tryptase-	272:280	arg1	type					317:320	tryptase-, chymase-, and carboxypeptidase A3 type	272:320	tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	272:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	0	15	theme	granule	101:107	arg1	modification					20:31	Proteolytic histone modification	0:31	Proteolytic histone modification by mast cell tryptase	0:53	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	0	15	theme	granule	101:107	arg1	protease					109:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	8	16	theme	secretory	1512:1520	arg1	axis					1557:1560	a secretory granule-derived serglycin-tryptase axis	1510:1560	a secretory granule-derived serglycin-tryptase axis	1510:1560	Together, these findings implicate histone proteolysis through a secretory granule-derived serglycin-tryptase axis as a novel principle for histone modification, during both cell homeostasis and cell death.
24478313	6	17	theme	viable	1256:1261	arg1	cells					1263:1267	viable cells	1256:1267	viable cells	1256:1267	Indeed, tryptase was found in the nucleus of viable cells and was shown to cleave core histones in their N-terminal tail.
24478313	8	18	theme	cell	1642:1645	arg1	death					1647:1651	cell death	1642:1651	cell death	1642:1651	Together, these findings implicate histone proteolysis through a secretory granule-derived serglycin-tryptase axis as a novel principle for histone modification, during both cell homeostasis and cell death.
24478313	5	19	from	impact	1185:1190	arg1	events					1203:1208	nuclear events	1195:1208	nuclear events	1195:1208	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	7	20	theme	serglycin-tryptase	1380:1397	arg1	axis					1399:1402	the serglycin-tryptase axis	1376:1402	the serglycin-tryptase axis	1376:1402	Moreover, it was shown that the absence of the serglycin-tryptase axis resulted in altered chromatin composition.
24478313	2	21	theme	cell	527:530	arg1	death					532:536	cell death	527:536	cell death	527:536	Apart from participating in extracellular processes, serglycin proteoglycan and one of its associated proteases, tryptase, are known to regulate cell death by promoting apoptosis over necrosis.
24478313	7	22	theme	altered	1416:1422	arg1	composition					1434:1444	altered chromatin composition	1416:1444	altered chromatin composition	1416:1444	Moreover, it was shown that the absence of the serglycin-tryptase axis resulted in altered chromatin composition.
24478313	7	23	theme	axis	1399:1402	arg1	absence					1365:1371	the absence	1361:1371	the absence of the serglycin-tryptase axis	1361:1402	Moreover, it was shown that the absence of the serglycin-tryptase axis resulted in altered chromatin composition.
24478313	2	24	theme	extracellular	410:422	arg1	processes					424:432	extracellular processes	410:432	extracellular processes	410:432	Apart from participating in extracellular processes, serglycin proteoglycan and one of its associated proteases, tryptase, are known to regulate cell death by promoting apoptosis over necrosis.
24478313	0	25	theme	histone	12:18	arg1	modification					20:31	Proteolytic histone modification	0:31	Proteolytic histone modification by mast cell tryptase	0:53	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	0	25	theme	histone	12:18	arg1	protease					109:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	7	26	theme	chromatin	1424:1432	arg1	composition					1434:1444	altered chromatin composition	1416:1444	altered chromatin composition	1416:1444	Moreover, it was shown that the absence of the serglycin-tryptase axis resulted in altered chromatin composition.
24478313	1	27	theme	cytoplasmic	177:187	arg1	granules					199:206	cytoplasmic secretory granules	177:206	cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	177:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	0	28	theme	Proteolytic	0:10	arg1	modification					20:31	Proteolytic histone modification	0:31	Proteolytic histone modification by mast cell tryptase	0:53	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	0	28	theme	Proteolytic	0:10	arg1	protease					109:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	1	29	theme	chymase-	283:290	arg1	type					317:320	tryptase-, chymase-, and carboxypeptidase A3 type	272:320	tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	272:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	8	30	link	granule-derived	1522:1536	arg1	axis					1557:1560	a secretory granule-derived serglycin-tryptase axis	1510:1560	a secretory granule-derived serglycin-tryptase axis	1510:1560	Together, these findings implicate histone proteolysis through a secretory granule-derived serglycin-tryptase axis as a novel principle for histone modification, during both cell homeostasis and cell death.
24478313	1	31	theme	secretory	189:197	arg1	granules					199:206	cytoplasmic secretory granules	177:206	cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	177:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	5	32	theme	homeostatic	1173:1183	arg1	impact					1185:1190	a homeostatic impact	1171:1190	a homeostatic impact on nuclear events	1171:1208	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	4	33	theme	histones	926:933	arg1	accumulation					905:916	a pronounced accumulation	892:916	a pronounced accumulation of core histones both in the cytosol and in the nucleus	892:972	During the cell death process, tryptase was found to relocalize from granules into the cytosol and nucleus, and it was found that the absence of tryptase was associated with a pronounced accumulation of core histones both in the cytosol and in the nucleus.
24478313	1	34	theme	granules	199:206	arg1	content					166:172	their high content	155:172	their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	155:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	1	34	theme	granules	199:206	arg1	feature					130:136	A hallmark feature	119:136	A hallmark feature of mast cells	119:150	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	8	35	theme	novel	1567:1571	arg1	proteolysis					1490:1500	histone proteolysis	1482:1500	histone proteolysis	1482:1500	Together, these findings implicate histone proteolysis through a secretory granule-derived serglycin-tryptase axis as a novel principle for histone modification, during both cell homeostasis and cell death.
24478313	8	35	theme	novel	1567:1571	arg1	principle					1573:1581	a novel principle	1565:1581	a novel principle for histone modification	1565:1606	Together, these findings implicate histone proteolysis through a secretory granule-derived serglycin-tryptase axis as a novel principle for histone modification, during both cell homeostasis and cell death.
24478313	4	36	theme	core	921:924	arg1	histones					926:933	core histones	921:933	core histones	921:933	During the cell death process, tryptase was found to relocalize from granules into the cytosol and nucleus, and it was found that the absence of tryptase was associated with a pronounced accumulation of core histones both in the cytosol and in the nucleus.
24478313	3	37	theme	proteolytic	665:675	arg1	targets					677:683	primary proteolytic targets	657:683	primary proteolytic targets for the serglycin-tryptase axis	657:715	Here we sought to outline the underlying mechanism and identify core histones as primary proteolytic targets for the serglycin-tryptase axis.
24478313	3	37	theme	proteolytic	665:675	arg1	histones					645:652	core histones	640:652	core histones	640:652	Here we sought to outline the underlying mechanism and identify core histones as primary proteolytic targets for the serglycin-tryptase axis.
24478313	0	38	theme	cell	41:44	arg1	tryptase					46:53	mast cell tryptase	36:53	mast cell tryptase	36:53	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	1	39	theme	carboxypeptidase	297:312	arg1	type					317:320	tryptase-, chymase-, and carboxypeptidase A3 type	272:320	tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	272:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	5	40	theme	baseline	1081:1088	arg1	conditions					1090:1099	baseline conditions	1081:1099	baseline conditions	1081:1099	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	3	41	theme	underlying	606:615	arg1	mechanism					617:625	the underlying mechanism	602:625	the underlying mechanism	602:625	Here we sought to outline the underlying mechanism and identify core histones as primary proteolytic targets for the serglycin-tryptase axis.
24478313	2	42	theme	serglycin	435:443	arg1	proteoglycan					445:456	serglycin proteoglycan	435:456	serglycin proteoglycan	435:456	Apart from participating in extracellular processes, serglycin proteoglycan and one of its associated proteases, tryptase, are known to regulate cell death by promoting apoptosis over necrosis.
24478313	0	43	theme	mast	36:39	arg1	tryptase					46:53	mast cell tryptase	36:53	mast cell tryptase	36:53	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	1	44	theme	A3	314:315	arg1	type					317:320	tryptase-, chymase-, and carboxypeptidase A3 type	272:320	tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	272:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	5	45	theme	defective	1021:1029	arg1	modification					1043:1054	defective proteolytic modification	1021:1054	defective proteolytic modification of core histones	1021:1071	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	1	46	theme	various	220:226	arg1	compounds					238:246	various preformed compounds	220:246	various preformed compounds	220:246	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	1	46	theme	various	220:226	arg1	proteases					259:267	proteases	259:267	proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	259:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	1	47	theme	type	317:320	arg1	proteases					259:267	proteases	259:267	proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	259:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	4	48	theme	pronounced	894:903	arg1	accumulation					905:916	a pronounced accumulation	892:916	a pronounced accumulation of core histones both in the cytosol and in the nucleus	892:972	During the cell death process, tryptase was found to relocalize from granules into the cytosol and nucleus, and it was found that the absence of tryptase was associated with a pronounced accumulation of core histones both in the cytosol and in the nucleus.
24478313	3	49	theme	primary	657:663	arg1	targets					677:683	primary proteolytic targets	657:683	primary proteolytic targets for the serglycin-tryptase axis	657:715	Here we sought to outline the underlying mechanism and identify core histones as primary proteolytic targets for the serglycin-tryptase axis.
24478313	3	49	theme	primary	657:663	arg1	histones					645:652	core histones	640:652	core histones	640:652	Here we sought to outline the underlying mechanism and identify core histones as primary proteolytic targets for the serglycin-tryptase axis.
24478313	5	50	theme	proteolytic	1031:1041	arg1	modification					1043:1054	defective proteolytic modification	1021:1054	defective proteolytic modification of core histones	1021:1071	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	8	51	theme	serglycin-tryptase	1538:1555	arg1	axis					1557:1560	a secretory granule-derived serglycin-tryptase axis	1510:1560	a secretory granule-derived serglycin-tryptase axis	1510:1560	Together, these findings implicate histone proteolysis through a secretory granule-derived serglycin-tryptase axis as a novel principle for histone modification, during both cell homeostasis and cell death.
24478313	1	52	theme	hallmark	121:128	arg1	content					166:172	their high content	155:172	their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	155:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	1	52	theme	hallmark	121:128	arg1	feature					130:136	A hallmark feature	119:136	A hallmark feature of mast cells	119:150	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	2	53	theme	proteases	484:492	arg1	one					462:464	one	462:464	one	462:464	Apart from participating in extracellular processes, serglycin proteoglycan and one of its associated proteases, tryptase, are known to regulate cell death by promoting apoptosis over necrosis.
24478313	2	53	theme	proteases	484:492	arg1	tryptase					495:502	tryptase	495:502	tryptase	495:502	Apart from participating in extracellular processes, serglycin proteoglycan and one of its associated proteases, tryptase, are known to regulate cell death by promoting apoptosis over necrosis.
24478313	2	53	theme	proteases	484:492	arg1	proteases					484:492	its associated proteases	469:492	its associated proteases	469:492	Apart from participating in extracellular processes, serglycin proteoglycan and one of its associated proteases, tryptase, are known to regulate cell death by promoting apoptosis over necrosis.
24478313	6	54	theme	N-terminal	1316:1325	arg1	tail					1327:1330	their N-terminal tail	1310:1330	their N-terminal tail	1310:1330	Indeed, tryptase was found in the nucleus of viable cells and was shown to cleave core histones in their N-terminal tail.
24478313	3	55	theme	serglycin-tryptase	693:710	arg1	axis					712:715	the serglycin-tryptase axis	689:715	the serglycin-tryptase axis	689:715	Here we sought to outline the underlying mechanism and identify core histones as primary proteolytic targets for the serglycin-tryptase axis.
24478313	5	56	theme	core	1059:1062	arg1	histones					1064:1071	core histones	1059:1071	core histones	1059:1071	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	4	57	theme	death	734:738	arg1	process					740:746	the cell death process	725:746	the cell death process	725:746	During the cell death process, tryptase was found to relocalize from granules into the cytosol and nucleus, and it was found that the absence of tryptase was associated with a pronounced accumulation of core histones both in the cytosol and in the nucleus.
24478313	8	58	theme	histone	1587:1593	arg1	modification					1595:1606	histone modification	1587:1606	histone modification	1587:1606	Together, these findings implicate histone proteolysis through a secretory granule-derived serglycin-tryptase axis as a novel principle for histone modification, during both cell homeostasis and cell death.
24478313	4	59	from	accumulation	905:916	arg1	nucleus					966:972	the nucleus	962:972	the nucleus	962:972	During the cell death process, tryptase was found to relocalize from granules into the cytosol and nucleus, and it was found that the absence of tryptase was associated with a pronounced accumulation of core histones both in the cytosol and in the nucleus.
24478313	4	59	from	accumulation	905:916	arg1	cytosol					947:953	the cytosol	943:953	the cytosol	943:953	During the cell death process, tryptase was found to relocalize from granules into the cytosol and nucleus, and it was found that the absence of tryptase was associated with a pronounced accumulation of core histones both in the cytosol and in the nucleus.
24478313	3	60	theme	core	640:643	arg1	targets					677:683	primary proteolytic targets	657:683	primary proteolytic targets for the serglycin-tryptase axis	657:715	Here we sought to outline the underlying mechanism and identify core histones as primary proteolytic targets for the serglycin-tryptase axis.
24478313	3	60	theme	core	640:643	arg1	histones					645:652	core histones	640:652	core histones	640:652	Here we sought to outline the underlying mechanism and identify core histones as primary proteolytic targets for the serglycin-tryptase axis.
24478313	1	61	theme	preformed	228:236	arg1	compounds					238:246	various preformed compounds	220:246	various preformed compounds	220:246	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	1	61	theme	preformed	228:236	arg1	proteases					259:267	proteases	259:267	proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	259:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	5	62	theme	histones	1064:1071	arg1	modification					1043:1054	defective proteolytic modification	1021:1054	defective proteolytic modification of core histones	1021:1071	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	5	63	theme	tryptase	989:996	arg1	deficiency					998:1007	tryptase deficiency	989:1007	tryptase deficiency	989:1007	Intriguingly, tryptase deficiency resulted in defective proteolytic modification of core histones even at baseline conditions, i.e. in the absence of cytotoxic agent, suggesting that tryptase has a homeostatic impact on nuclear events.
24478313	1	64	theme	mast	141:144	arg1	cells					146:150	mast cells	141:150	mast cells	141:150	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	0	65	theme	serglycin	58:66	arg1	modification					20:31	Proteolytic histone modification	0:31	Proteolytic histone modification by mast cell tryptase	0:53	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	0	65	theme	serglycin	58:66	arg1	protease					109:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	a serglycin proteoglycan-dependent secretory granule protease	56:116	Proteolytic histone modification by mast cell tryptase, a serglycin proteoglycan-dependent secretory granule protease.
24478313	1	66	theme	cells	146:150	arg1	content					166:172	their high content	155:172	their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan	155:379	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	1	66	theme	cells	146:150	arg1	feature					130:136	A hallmark feature	119:136	A hallmark feature of mast cells	119:150	A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled with various preformed compounds, including proteases of tryptase-, chymase-, and carboxypeptidase A3 type that are electrostatically bound to serglycin proteoglycan.
24478313	6	67	theme	core	1293:1296	arg1	histones					1298:1305	core histones	1293:1305	core histones	1293:1305	Indeed, tryptase was found in the nucleus of viable cells and was shown to cleave core histones in their N-terminal tail.
24478313	8	68	theme	histone	1482:1488	arg1	proteolysis					1490:1500	histone proteolysis	1482:1500	histone proteolysis	1482:1500	Together, these findings implicate histone proteolysis through a secretory granule-derived serglycin-tryptase axis as a novel principle for histone modification, during both cell homeostasis and cell death.
24478313	8	68	theme	histone	1482:1488	arg1	principle					1573:1581	a novel principle	1565:1581	a novel principle for histone modification	1565:1606	Together, these findings implicate histone proteolysis through a secretory granule-derived serglycin-tryptase axis as a novel principle for histone modification, during both cell homeostasis and cell death.
24478313	2	69	theme	associated	473:482	arg1	proteases					484:492	its associated proteases	469:492	its associated proteases	469:492	Apart from participating in extracellular processes, serglycin proteoglycan and one of its associated proteases, tryptase, are known to regulate cell death by promoting apoptosis over necrosis.
24478313	6	70	located	found	1232:1236	arg1	nucleus					1245:1251	the nucleus	1241:1251	the nucleus of viable cells	1241:1267	Indeed, tryptase was found in the nucleus of viable cells and was shown to cleave core histones in their N-terminal tail.
24478313	6	70	located	found	1232:1236	arg2	tryptase					1219:1226	tryptase	1219:1226	tryptase	1219:1226	Indeed, tryptase was found in the nucleus of viable cells and was shown to cleave core histones in their N-terminal tail.
25562119	6	0	theme	cell	1245:1248	arg1	proliferation					1250:1262	cell proliferation	1245:1262	cell proliferation	1245:1262	Culture of human osteoblast cells on these scaffolds also demonstrated a great improvement of cell proliferation and cell viability.
25562119	8	1	theme	bone	1826:1829	arg1	engineering					1838:1848	bone tissue engineering	1826:1848	bone tissue engineering	1826:1848	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	7	2	theme	phosphate	1397:1405	arg1	crystals					1407:1414	the calcium phosphate crystals	1385:1414	the calcium phosphate crystals	1385:1414	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	8	3	theme	tissue	1831:1836	arg1	engineering					1838:1848	bone tissue engineering	1826:1848	bone tissue engineering	1826:1848	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	4	4	theme	structural	668:677	arg1	characterization					679:694	structural characterization	668:694	structural characterization	668:694	Results of structural characterization revealed that the scaffolds printed with 2.5 wt% alginate-modified TCP powders presented a uniformly distributed and interfusing alginate TCP network.
25562119	8	5	theme	Mohr	1679:1682	arg1	criterion					1693:1701	the applied Mohr fracture criterion	1667:1701	the applied Mohr fracture criterion	1667:1701	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	2	6	theme	scaffolds	331:339	arg1	performance					300:310	the functional performance	285:310	the functional performance of brittle ceramic scaffolds	285:339	Herein, we aimed to improve the functional performance of brittle ceramic scaffolds by developing a promising biopolymer-ceramic network.
25562119	3	7	theme	vacuum	571:576	arg1	infiltration					578:589	vacuum infiltration	571:589	vacuum infiltration	571:589	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	5	8	theme	significant	878:888	arg1	increase					890:897	a significant increase	876:897	a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt%	876:1023	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	7	9	theme	isolated	1342:1349	arg1	gels					1360:1363	isolated alginate gels	1342:1363	isolated alginate gels	1342:1363	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	0	10	theme	alginate-TCP	26:37	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of individual alginate-TCP	0:37	Fabrication of individual alginate-TCP scaffolds for bone tissue engineering by means of powder printing.
25562119	5	11	from	increase	890:897	arg1	reliability					934:944	reliability	934:944	reliability	934:944	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	11	from	increase	890:897	arg1	failure					922:928	energy to failure	912:928	energy to failure	912:928	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	11	from	increase	890:897	arg1	strength					902:909	strength	902:909	strength	902:909	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	1	12	theme	bone	238:241	arg1	regeneration					243:254	bone regeneration	238:254	bone regeneration	238:254	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration.
25562119	7	13	theme	scaffolds	1331:1339	arg1	case					1297:1300	the case	1293:1300	the case of powder-mixed alginate TCP scaffolds	1293:1339	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	6	14	theme	viability	1273:1281	arg1	improvement					1230:1240	a great improvement	1222:1240	a great improvement of cell proliferation and cell viability	1222:1281	Culture of human osteoblast cells on these scaffolds also demonstrated a great improvement of cell proliferation and cell viability.
25562119	3	15	theme	TCP	602:604	arg1	scaffolds					606:614	printed TCP scaffolds	594:614	printed TCP scaffolds with an alginate solution	594:640	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	8	16	theme	powder-modified	1734:1748	arg1	scaffolds					1750:1758	the powder-modified scaffolds	1730:1758	the powder-modified scaffolds	1730:1758	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	5	17	theme	scaffolds	965:973	arg1	reliability					934:944	reliability	934:944	reliability	934:944	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	17	theme	scaffolds	965:973	arg1	failure					922:928	energy to failure	912:928	energy to failure	912:928	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	17	theme	scaffolds	965:973	arg1	strength					902:909	strength	902:909	strength	902:909	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	7	18	theme	alginate	1318:1325	arg1	scaffolds					1331:1339	powder-mixed alginate TCP scaffolds	1305:1339	powder-mixed alginate TCP scaffolds	1305:1339	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	6	19	theme	osteoblast	1168:1177	arg1	cells					1179:1183	human osteoblast cells	1162:1183	human osteoblast cells	1162:1183	Culture of human osteoblast cells on these scaffolds also demonstrated a great improvement of cell proliferation and cell viability.
25562119	4	20	theme	%	743:743	arg1	powders					767:773	2.5 wt% alginate-modified TCP powders	737:773	2.5 wt% alginate-modified TCP powders	737:773	Results of structural characterization revealed that the scaffolds printed with 2.5 wt% alginate-modified TCP powders presented a uniformly distributed and interfusing alginate TCP network.
25562119	1	21	theme	phosphate	141:149	arg1	scaffolds					161:169	polymer-calcium phosphate composite scaffolds	125:169	polymer-calcium phosphate composite scaffolds with tailored architectures and properties	125:212	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration.
25562119	3	22	theme	alginate	624:631	arg1	solution					633:640	an alginate solution	621:640	an alginate solution	621:640	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	8	23	theme	wt	1769:1770	arg1	alginate					1773:1780	2.5 wt% alginate	1765:1780	2.5 wt% alginate	1765:1780	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	1	24	theme	scaffolds	161:169	arg1	development					110:120	The development	106:120	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties	106:212	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration.
25562119	7	25	theme	alginate	1541:1548	arg1	film					1550:1553	a thin alginate film	1534:1553	a thin alginate film	1534:1553	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	6	26	from	Culture	1151:1157	arg1	scaffolds					1194:1202	these scaffolds	1188:1202	these scaffolds	1188:1202	Culture of human osteoblast cells on these scaffolds also demonstrated a great improvement of cell proliferation and cell viability.
25562119	1	27	theme	tailored	176:183	arg1	architectures					185:197	tailored architectures	176:197	tailored architectures	176:197	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration.
25562119	7	28	theme	TCP	1327:1329	arg1	scaffolds					1331:1339	powder-mixed alginate TCP scaffolds	1305:1339	powder-mixed alginate TCP scaffolds	1305:1339	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	0	29	theme	powder	89:94	arg1	printing					96:103	powder printing	89:103	powder printing	89:103	Fabrication of individual alginate-TCP scaffolds for bone tissue engineering by means of powder printing.
25562119	1	30	with	scaffolds	161:169	arg1	properties					203:212	properties	203:212	properties	203:212	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration.
25562119	1	30	with	scaffolds	161:169	arg1	architectures					185:197	tailored architectures	176:197	tailored architectures	176:197	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration.
25562119	7	31	theme	scaffold	1520:1527	arg1	surface					1482:1488	surface	1482:1488	surface	1482:1488	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	7	31	theme	scaffold	1520:1527	arg1	pores					1503:1507	internal pores	1494:1507	internal pores	1494:1507	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	5	32	theme	wt	1021:1022	arg1	%					1023:1023	2.5 wt%	1017:1023	2.5 wt%	1017:1023	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	4	33	theme	TCP	763:765	arg1	powders					767:773	2.5 wt% alginate-modified TCP powders	737:773	2.5 wt% alginate-modified TCP powders	737:773	Results of structural characterization revealed that the scaffolds printed with 2.5 wt% alginate-modified TCP powders presented a uniformly distributed and interfusing alginate TCP network.
25562119	5	34	theme	Mechanical	847:856	arg1	results					858:864	Mechanical results	847:864	Mechanical results	847:864	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	35	contain	containing	1080:1089	arg2	%					1095:1095	5 wt%	1091:1095	5 wt%	1091:1095	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	35	contain	containing	1080:1089	arg2	%					1106:1106	7.5 wt%	1100:1106	7.5 wt% in the educts, in both dry and wet states	1100:1148	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	35	contain	containing	1080:1089	arg1	scaffolds					1070:1078	TCP scaffolds	1066:1078	TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states	1066:1148	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	36	dep	failure	922:928	arg1	to					919:920	to	919:920	to	919:920	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	0	37	theme	individual	15:24	arg1	alginate-TCP					26:37	individual alginate-TCP	15:37	individual alginate-TCP	15:37	Fabrication of individual alginate-TCP scaffolds for bone tissue engineering by means of powder printing.
25562119	3	38	theme	powder	541:546	arg1	mixture					499:505	a 60:40 mixture	491:505	a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder	491:546	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	3	39	theme	composition	465:475	arg1	strategies					417:426	two strategies	413:426	two strategies	413:426	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	3	39	theme	composition	465:475	arg1	printing					444:451	direct printing	437:451	direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder	437:546	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	3	39	theme	composition	465:475	arg1	powder					561:566	alginate powder	552:566	alginate powder	552:566	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	3	39	theme	composition	465:475	arg1	infiltration					578:589	vacuum infiltration	571:589	vacuum infiltration	571:589	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	5	40	from	educts	1115:1120	arg1	states					1143:1148	both dry and wet states	1126:1148	both dry and wet states	1126:1148	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	7	41	theme	internal	1494:1501	arg1	pores					1503:1507	internal pores	1494:1507	internal pores	1494:1507	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	2	42	theme	biopolymer-ceramic	367:384	arg1	network					386:392	a promising biopolymer-ceramic network	355:392	a promising biopolymer-ceramic network	355:392	Herein, we aimed to improve the functional performance of brittle ceramic scaffolds by developing a promising biopolymer-ceramic network.
25562119	0	43	theme	bone	53:56	arg1	engineering					65:75	bone tissue engineering	53:75	bone tissue engineering by means of powder printing	53:103	Fabrication of individual alginate-TCP scaffolds for bone tissue engineering by means of powder printing.
25562119	4	44	theme	alginate	825:832	arg1	network					838:844	a uniformly distributed and interfusing alginate TCP network	785:844	a uniformly distributed and interfusing alginate TCP network	785:844	Results of structural characterization revealed that the scaffolds printed with 2.5 wt% alginate-modified TCP powders presented a uniformly distributed and interfusing alginate TCP network.
25562119	3	45	theme	60:40	493:497	arg1	mixture					499:505	a 60:40 mixture	491:505	a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder	491:546	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	8	46	theme	critical	1602:1609	arg1	conditions					1620:1629	the scaffolds' critical fracture conditions	1587:1629	the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion	1587:1701	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	3	47	theme	TCP	536:538	arg1	powder					541:546	α/β-tricalcium phosphate (TCP) powder	510:546	α/β-tricalcium phosphate (TCP) powder	510:546	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	5	48	from	content	992:998	arg1	educts					1007:1012	the educts	1003:1012	the educts of 2.5 wt%	1003:1023	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	49	from	%	1106:1106	arg1	educts					1115:1120	the educts	1111:1120	the educts	1111:1120	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	6	50	theme	proliferation	1250:1262	arg1	improvement					1230:1240	a great improvement	1222:1240	a great improvement of cell proliferation and cell viability	1222:1281	Culture of human osteoblast cells on these scaffolds also demonstrated a great improvement of cell proliferation and cell viability.
25562119	3	51	theme	phosphate	525:533	arg1	powder					541:546	α/β-tricalcium phosphate (TCP) powder	510:546	α/β-tricalcium phosphate (TCP) powder	510:546	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	3	52	theme	alginate	552:559	arg1	powder					561:566	alginate powder	552:566	alginate powder	552:566	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	3	52	theme	alginate	552:559	arg1	strategies					417:426	two strategies	413:426	two strategies	413:426	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	8	53	theme	stress	1650:1655	arg1	states					1657:1662	more complex stress states	1637:1662	more complex stress states	1637:1662	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	7	54	dep	surface	1482:1488	arg1	the					1478:1480	the	1478:1480	the	1478:1480	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	7	55	theme	vacuum-infiltration	1421:1439	arg1	strategy					1441:1448	the vacuum-infiltration strategy	1417:1448	the vacuum-infiltration strategy	1417:1448	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	6	56	theme	great	1224:1228	arg1	improvement					1230:1240	a great improvement	1222:1240	a great improvement of cell proliferation and cell viability	1222:1281	Culture of human osteoblast cells on these scaffolds also demonstrated a great improvement of cell proliferation and cell viability.
25562119	2	57	theme	functional	289:298	arg1	performance					300:310	the functional performance	285:310	the functional performance of brittle ceramic scaffolds	285:339	Herein, we aimed to improve the functional performance of brittle ceramic scaffolds by developing a promising biopolymer-ceramic network.
25562119	7	58	theme	calcium	1389:1395	arg1	crystals					1407:1414	the calcium phosphate crystals	1385:1414	the calcium phosphate crystals	1385:1414	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	8	59	theme	applied	1671:1677	arg1	criterion					1693:1701	the applied Mohr fracture criterion	1667:1701	the applied Mohr fracture criterion	1667:1701	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	5	60	theme	energy	912:917	arg1	failure					922:928	energy to failure	912:928	energy to failure	912:928	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	4	61	theme	characterization	679:694	arg1	Results					657:663	Results	657:663	Results of structural characterization	657:694	Results of structural characterization revealed that the scaffolds printed with 2.5 wt% alginate-modified TCP powders presented a uniformly distributed and interfusing alginate TCP network.
25562119	2	62	theme	ceramic	323:329	arg1	scaffolds					331:339	brittle ceramic scaffolds	315:339	brittle ceramic scaffolds	315:339	Herein, we aimed to improve the functional performance of brittle ceramic scaffolds by developing a promising biopolymer-ceramic network.
25562119	8	63	theme	fracture	1684:1691	arg1	criterion					1693:1701	the applied Mohr fracture criterion	1667:1701	the applied Mohr fracture criterion	1667:1701	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	1	64	theme	great	218:222	arg1	potential					224:232	great potential	218:232	great potential for bone regeneration	218:254	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration.
25562119	2	65	theme	promising	357:365	arg1	network					386:392	a promising biopolymer-ceramic network	355:392	a promising biopolymer-ceramic network	355:392	Herein, we aimed to improve the functional performance of brittle ceramic scaffolds by developing a promising biopolymer-ceramic network.
25562119	3	66	theme	scaffolds	606:614	arg1	strategies					417:426	two strategies	413:426	two strategies	413:426	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	3	66	theme	scaffolds	606:614	arg1	printing					444:451	direct printing	437:451	direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder	437:546	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	3	66	theme	scaffolds	606:614	arg1	powder					561:566	alginate powder	552:566	alginate powder	552:566	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	3	66	theme	scaffolds	606:614	arg1	infiltration					578:589	vacuum infiltration	571:589	vacuum infiltration	571:589	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	7	67	theme	alginate	1351:1358	arg1	gels					1360:1363	isolated alginate gels	1342:1363	isolated alginate gels	1342:1363	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	5	68	theme	wt	1093:1094	arg1	%					1095:1095	5 wt%	1091:1095	5 wt%	1091:1095	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	3	69	theme	printed	594:600	arg1	scaffolds					606:614	printed TCP scaffolds	594:614	printed TCP scaffolds with an alginate solution	594:640	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	5	70	theme	powder-modified	949:963	arg1	scaffolds					965:973	powder-modified scaffolds	949:973	powder-modified scaffolds with an alginate content in the educts of 2.5 wt%	949:1023	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	6	71	theme	cells	1179:1183	arg1	Culture					1151:1157	Culture	1151:1157	Culture of human osteoblast cells on these scaffolds	1151:1202	Culture of human osteoblast cells on these scaffolds also demonstrated a great improvement of cell proliferation and cell viability.
25562119	6	72	theme	cell	1268:1271	arg1	viability					1273:1281	cell viability	1268:1281	cell viability	1268:1281	Culture of human osteoblast cells on these scaffolds also demonstrated a great improvement of cell proliferation and cell viability.
25562119	1	73	theme	polymer-calcium	125:139	arg1	scaffolds					161:169	polymer-calcium phosphate composite scaffolds	125:169	polymer-calcium phosphate composite scaffolds with tailored architectures and properties	125:212	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration.
25562119	6	74	theme	human	1162:1166	arg1	cells					1179:1183	human osteoblast cells	1162:1183	human osteoblast cells	1162:1183	Culture of human osteoblast cells on these scaffolds also demonstrated a great improvement of cell proliferation and cell viability.
25562119	4	75	theme	wt	741:742	arg1	powders					767:773	2.5 wt% alginate-modified TCP powders	737:773	2.5 wt% alginate-modified TCP powders	737:773	Results of structural characterization revealed that the scaffolds printed with 2.5 wt% alginate-modified TCP powders presented a uniformly distributed and interfusing alginate TCP network.
25562119	5	76	theme	TCP	1066:1068	arg1	scaffolds					1070:1078	TCP scaffolds	1066:1078	TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states	1066:1148	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	8	77	theme	scaffolds	1750:1758	arg1	potential					1717:1725	the potential	1713:1725	the potential of the powder-modified scaffolds	1713:1758	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	1	78	theme	composite	151:159	arg1	scaffolds					161:169	polymer-calcium phosphate composite scaffolds	125:169	polymer-calcium phosphate composite scaffolds with tailored architectures and properties	125:212	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration.
25562119	7	79	theme	thin	1536:1539	arg1	film					1550:1553	a thin alginate film	1534:1553	a thin alginate film	1534:1553	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	7	80	theme	powder-mixed	1305:1316	arg1	scaffolds					1331:1339	powder-mixed alginate TCP scaffolds	1305:1339	powder-mixed alginate TCP scaffolds	1305:1339	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	8	81	theme	2.5	1765:1767	arg1	wt					1769:1770	wt	1769:1770	wt	1769:1770	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	8	82	theme	%	1771:1771	arg1	alginate					1773:1780	2.5 wt% alginate	1765:1780	2.5 wt% alginate	1765:1780	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	2	83	theme	brittle	315:321	arg1	scaffolds					331:339	brittle ceramic scaffolds	315:339	brittle ceramic scaffolds	315:339	Herein, we aimed to improve the functional performance of brittle ceramic scaffolds by developing a promising biopolymer-ceramic network.
25562119	7	84	theme	TCP	1516:1518	arg1	scaffold					1520:1527	the TCP scaffold	1512:1527	the TCP scaffold	1512:1527	While in the case of powder-mixed alginate TCP scaffolds, isolated alginate gels were formed between the calcium phosphate crystals, the vacuum-infiltration strategy resulted in the covering of the surface and internal pores of the TCP scaffold with a thin alginate film.
25562119	3	85	with	scaffolds	606:614	arg1	solution					633:640	an alginate solution	621:640	an alginate solution	621:640	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	4	86	theme	distributed	797:807	arg1	network					838:844	a uniformly distributed and interfusing alginate TCP network	785:844	a uniformly distributed and interfusing alginate TCP network	785:844	Results of structural characterization revealed that the scaffolds printed with 2.5 wt% alginate-modified TCP powders presented a uniformly distributed and interfusing alginate TCP network.
25562119	3	87	theme	powder	458:463	arg1	composition					465:475	a powder composition	456:475	a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder	456:546	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	5	88	theme	wt	1104:1105	arg1	%					1106:1106	7.5 wt%	1100:1106	7.5 wt% in the educts, in both dry and wet states	1100:1148	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	89	theme	alginate	983:990	arg1	content					992:998	an alginate content	980:998	an alginate content in the educts of 2.5 wt%	980:1023	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	90	with	scaffolds	965:973	arg1	content					992:998	an alginate content	980:998	an alginate content in the educts of 2.5 wt%	980:1023	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	4	91	theme	alginate-modified	745:761	arg1	powders					767:773	2.5 wt% alginate-modified TCP powders	737:773	2.5 wt% alginate-modified TCP powders	737:773	Results of structural characterization revealed that the scaffolds printed with 2.5 wt% alginate-modified TCP powders presented a uniformly distributed and interfusing alginate TCP network.
25562119	5	92	from	%	1095:1095	arg1	educts					1115:1120	the educts	1111:1120	the educts	1111:1120	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	93	theme	wet	1139:1141	arg1	states					1143:1148	both dry and wet states	1126:1148	both dry and wet states	1126:1148	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	1	94	contain	has	214:216	arg1	development					110:120	The development	106:120	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties	106:212	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration.
25562119	1	94	contain	has	214:216	arg2	potential					224:232	great potential	218:232	great potential for bone regeneration	218:254	The development of polymer-calcium phosphate composite scaffolds with tailored architectures and properties has great potential for bone regeneration.
25562119	5	95	theme	pure	1042:1045	arg1	TCP					1047:1049	pure TCP	1042:1049	pure TCP	1042:1049	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	3	96	theme	direct	437:442	arg1	printing					444:451	direct printing	437:451	direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder	437:546	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	3	96	theme	direct	437:442	arg1	strategies					417:426	two strategies	413:426	two strategies	413:426	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	0	97	theme	tissue	58:63	arg1	engineering					65:75	bone tissue engineering	53:75	bone tissue engineering by means of powder printing	53:103	Fabrication of individual alginate-TCP scaffolds for bone tissue engineering by means of powder printing.
25562119	3	98	theme	α/β-tricalcium	510:523	arg1	powder					541:546	α/β-tricalcium phosphate (TCP) powder	510:546	α/β-tricalcium phosphate (TCP) powder	510:546	For this purpose, two strategies, namely, direct printing of a powder composition consisting of a 60:40 mixture of α/β-tricalcium phosphate (TCP) powder and alginate powder or vacuum infiltration of printed TCP scaffolds with an alginate solution, were tracked.
25562119	8	99	theme	fracture	1611:1618	arg1	conditions					1620:1629	the scaffolds' critical fracture conditions	1587:1629	the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion	1587:1701	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
25562119	5	100	theme	dry	1131:1133	arg1	states					1143:1148	both dry and wet states	1126:1148	both dry and wet states	1126:1148	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	5	101	theme	%	1023:1023	arg1	educts					1007:1012	the educts	1003:1012	the educts of 2.5 wt%	1003:1023	Mechanical results indicated a significant increase in strength, energy to failure and reliability of powder-modified scaffolds with an alginate content in the educts of 2.5 wt% when compared to pure TCP, as well as to TCP scaffolds containing 5 wt% or 7.5 wt% in the educts, in both dry and wet states.
25562119	4	102	theme	interfusing	813:823	arg1	network					838:844	a uniformly distributed and interfusing alginate TCP network	785:844	a uniformly distributed and interfusing alginate TCP network	785:844	Results of structural characterization revealed that the scaffolds printed with 2.5 wt% alginate-modified TCP powders presented a uniformly distributed and interfusing alginate TCP network.
25562119	4	103	theme	TCP	834:836	arg1	network					838:844	a uniformly distributed and interfusing alginate TCP network	785:844	a uniformly distributed and interfusing alginate TCP network	785:844	Results of structural characterization revealed that the scaffolds printed with 2.5 wt% alginate-modified TCP powders presented a uniformly distributed and interfusing alginate TCP network.
25562119	8	104	theme	complex	1642:1648	arg1	states					1657:1662	more complex stress states	1637:1662	more complex stress states	1637:1662	Furthermore, the prediction of the scaffolds' critical fracture conditions under more complex stress states by the applied Mohr fracture criterion confirmed the potential of the powder-modified scaffolds with 2.5 wt% alginate in the educts as structural biomaterial for bone tissue engineering.
24997896	0	0	theme	adsorbent	82:90	arg1	eco-friendly					69:80	a new eco-friendly	63:80	a new eco-friendly adsorbent to remove micronutrients from agroindustrial effluents	63:145	Formulation of an alginate-vineyard pruning waste composite as a new eco-friendly adsorbent to remove micronutrients from agroindustrial effluents.
24997896	1	1	theme	nutrients	342:350	arg1	removal					321:327	the removal	317:327	the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater)	317:464	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	2	2	theme	adsorbent	544:552	arg1	adsorbent					544:552	adsorbent	544:552	adsorbent	544:552	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	2	theme	adsorbent	544:552	arg1	alginate					571:578	sodium alginate	564:578	sodium alginate (1-5%)	564:585	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	2	theme	adsorbent	544:552	arg1	chloride					599:606	calcium chloride	591:606	calcium chloride (0.05-0.9M)	591:618	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	2	theme	adsorbent	544:552	arg1	0.05-0.9M					609:617	0.05-0.9M	609:617	0.05-0.9M	609:617	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	2	theme	adsorbent	544:552	arg1	%					560:560	0.5-2%	555:560	0.5-2%	555:560	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	2	theme	adsorbent	544:552	arg1	%					584:584	1-5%	581:584	1-5%	581:584	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	2	theme	adsorbent	544:552	arg1	amounts					522:528	the amounts	518:528	the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite	518:647	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	5	3	theme	optimal	1156:1162	arg1	mixture					1164:1170	the optimal mixture	1152:1170	the optimal mixture	1152:1170	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	5	4	theme	sodium	1188:1193	arg1	alginate					1195:1202	sodium alginate	1188:1202	sodium alginate	1188:1202	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	1	5	theme	alginate	255:262	arg1	beads					264:268	calcium alginate beads	247:268	calcium alginate beads	247:268	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	5	6	theme	cellulosic	1238:1247	arg1	waste					1266:1270	cellulosic vineyard pruning waste	1238:1270	0.5% cellulosic vineyard pruning waste	1233:1270	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	5	7	theme	0.475M	1205:1210	arg1	chloride					1220:1227	0.475M calcium chloride	1205:1227	0.475M calcium chloride	1205:1227	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	1	8	theme	vineyard	175:182	arg1	waste					192:196	vineyard pruning waste	175:196	vineyard pruning waste (free of hemicellulosic sugars)	175:228	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	4	9	theme	cellulosic	901:910	arg1	waste					929:933	0.5% cellulosic vineyard pruning waste	896:933	0.5% cellulosic vineyard pruning waste	896:933	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	5	10	theme	pruning	1258:1264	arg1	waste					1266:1270	cellulosic vineyard pruning waste	1238:1270	0.5% cellulosic vineyard pruning waste	1233:1270	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	1	11	theme	micronutrients	356:369	arg1	removal					321:327	the removal	317:327	the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater)	317:464	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	4	12	theme	contaminants	782:793	arg1	adsorption					759:768	the adsorption	755:768	the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN)	755:819	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	2	13	theme	alginate	571:578	arg1	adsorbent					544:552	adsorbent	544:552	adsorbent	544:552	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	13	theme	alginate	571:578	arg1	alginate					571:578	sodium alginate	564:578	sodium alginate (1-5%)	564:585	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	13	theme	alginate	571:578	arg1	chloride					599:606	calcium chloride	591:606	calcium chloride (0.05-0.9M)	591:618	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	13	theme	alginate	571:578	arg1	0.05-0.9M					609:617	0.05-0.9M	609:617	0.05-0.9M	609:617	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	13	theme	alginate	571:578	arg1	%					560:560	0.5-2%	555:560	0.5-2%	555:560	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	13	theme	alginate	571:578	arg1	%					584:584	1-5%	581:584	1-5%	581:584	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	13	theme	alginate	571:578	arg1	amounts					522:528	the amounts	518:528	the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite	518:647	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	4	14	theme	0.9M	1045:1048	arg1	chloride					1058:1065	0.9M calcium chloride	1045:1065	0.9M calcium chloride	1045:1065	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	15	theme	%	899:899	arg1	waste					929:933	0.5% cellulosic vineyard pruning waste	896:933	0.5% cellulosic vineyard pruning waste	896:933	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	16	theme	cationic	773:780	arg1	K					804:804	K	804:804	K	804:804	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	16	theme	cationic	773:780	arg1	N-NH4					807:811	N-NH4	807:811	N-NH4	807:811	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	16	theme	cationic	773:780	arg1	Mg					796:797	Mg	796:797	Mg	796:797	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	16	theme	cationic	773:780	arg1	Zn					800:801	Zn	800:801	Zn	800:801	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	16	theme	cationic	773:780	arg1	TN					817:818	TN	817:818	TN	817:818	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	16	theme	cationic	773:780	arg1	contaminants					782:793	cationic contaminants	773:793	cationic contaminants (Mg, Zn, K, N-NH4 and TN)	773:819	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	2	17	theme	sodium	564:569	arg1	alginate					571:578	sodium alginate	564:578	sodium alginate (1-5%)	564:585	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	17	theme	sodium	564:569	arg1	%					584:584	1-5%	581:584	1-5%	581:584	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	4	18	theme	5	849:849	arg1	%					850:850	%	850:850	%	850:850	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	3	19	theme	target	725:730	arg1	contaminant					732:742	the target contaminant	721:742	the target contaminant	721:742	The optimal formulation of the adsorbent composite varied depending on the target contaminant.
24997896	5	20	theme	calcium	1212:1218	arg1	chloride					1220:1227	0.475M calcium chloride	1205:1227	0.475M calcium chloride	1205:1227	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	21	theme	compounds	967:975	arg1	removal					948:954	removal	948:954	removal of anionic compounds (P, SO4 and PO4)	948:992	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	5	22	theme	alginate	1195:1202	arg1	alginate					1195:1202	sodium alginate	1188:1202	sodium alginate	1188:1202	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	5	22	theme	alginate	1195:1202	arg1	chloride					1220:1227	0.475M calcium chloride	1205:1227	0.475M calcium chloride	1205:1227	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	5	22	theme	alginate	1195:1202	arg1	%					1236:1236	0.5%	1233:1236	0.5% cellulosic vineyard pruning waste	1233:1270	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	5	22	theme	alginate	1195:1202	arg1	%					1183:1183	3%	1182:1183	3% of sodium alginate	1182:1202	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	23	theme	anionic	959:965	arg1	compounds					967:975	anionic compounds	959:975	anionic compounds (P, SO4 and PO4)	959:992	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	1	24	theme	pruning	184:190	arg1	waste					192:196	vineyard pruning waste	175:196	vineyard pruning waste (free of hemicellulosic sugars)	175:228	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	4	25	theme	sodium	852:857	arg1	alginate					859:866	5% sodium alginate	849:866	5% sodium alginate	849:866	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	26	theme	%	850:850	arg1	alginate					859:866	5% sodium alginate	849:866	5% sodium alginate	849:866	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	1	27	theme	waste	192:196	arg1	fraction					163:170	The cellulosic fraction	148:170	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars)	148:228	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	27	theme	waste	192:196	arg1	adsorbent					303:311	adsorbent	303:311	adsorbent	303:311	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	4	28	theme	0.05M	869:873	arg1	chloride					883:890	0.05M calcium chloride	869:890	0.05M calcium chloride	869:890	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	2	29	theme	0.5-2	555:559	arg1	adsorbent					544:552	adsorbent	544:552	adsorbent	544:552	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	29	theme	0.5-2	555:559	arg1	%					560:560	0.5-2%	555:560	0.5-2%	555:560	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	4	30	theme	best	826:829	arg1	mixture					831:837	the best mixture	822:837	the best mixture	822:837	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	5	31	theme	winery	1133:1138	arg1	wastewater					1140:1149	the winery wastewater	1129:1149	the winery wastewater	1129:1149	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	5	32	theme	vineyard	1249:1256	arg1	waste					1266:1270	cellulosic vineyard pruning waste	1238:1270	0.5% cellulosic vineyard pruning waste	1233:1270	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	33	theme	%	1026:1026	arg1	alginate					1035:1042	1% sodium alginate	1025:1042	1% sodium alginate	1025:1042	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	0	34	theme	alginate-vineyard	18:34	arg1	composite					50:58	an alginate-vineyard pruning waste composite	15:58	an alginate-vineyard pruning waste composite	15:58	Formulation of an alginate-vineyard pruning waste composite as a new eco-friendly adsorbent to remove micronutrients from agroindustrial effluents.
24997896	3	35	theme	optimal	654:660	arg1	formulation					662:672	The optimal formulation	650:672	The optimal formulation of the adsorbent composite	650:699	The optimal formulation of the adsorbent composite varied depending on the target contaminant.
24997896	4	36	theme	1	1025:1025	arg1	%					1026:1026	%	1026:1026	%	1026:1026	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	37	dep	contaminants	782:793	arg1	K					804:804	K	804:804	K	804:804	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	37	dep	contaminants	782:793	arg1	N-NH4					807:811	N-NH4	807:811	N-NH4	807:811	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	37	dep	contaminants	782:793	arg1	Mg					796:797	Mg	796:797	Mg	796:797	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	37	dep	contaminants	782:793	arg1	Zn					800:801	Zn	800:801	Zn	800:801	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	37	dep	contaminants	782:793	arg1	TN					817:818	TN	817:818	TN	817:818	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	37	dep	contaminants	782:793	arg1	contaminants					782:793	cationic contaminants	773:793	cationic contaminants (Mg, Zn, K, N-NH4 and TN)	773:819	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	1	38	from	effluent	437:444	arg1	removal					321:327	the removal	317:327	the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater)	317:464	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	4	39	theme	calcium	875:881	arg1	chloride					883:890	0.05M calcium chloride	869:890	0.05M calcium chloride	869:890	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	0	40	theme	agroindustrial	122:135	arg1	effluents					137:145	agroindustrial effluents	122:145	agroindustrial effluents	122:145	Formulation of an alginate-vineyard pruning waste composite as a new eco-friendly adsorbent to remove micronutrients from agroindustrial effluents.
24997896	4	41	theme	cellulosic	1076:1085	arg1	waste					1104:1108	0.5% cellulosic vineyard pruning waste	1071:1108	0.5% cellulosic vineyard pruning waste	1071:1108	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	1	42	theme	hemicellulosic	207:220	arg1	sugars					222:227	hemicellulosic sugars	207:227	hemicellulosic sugars	207:227	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	0	43	theme	waste	44:48	arg1	composite					50:58	an alginate-vineyard pruning waste composite	15:58	an alginate-vineyard pruning waste composite	15:58	Formulation of an alginate-vineyard pruning waste composite as a new eco-friendly adsorbent to remove micronutrients from agroindustrial effluents.
24997896	1	44	dep	nutrients	342:350	arg1	K					383:383	K	383:383	K	383:383	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	44	dep	nutrients	342:350	arg1	TN					398:399	TN	398:399	TN	398:399	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	44	dep	nutrients	342:350	arg1	Mg					372:373	Mg	372:373	Mg	372:373	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	44	dep	nutrients	342:350	arg1	TC					402:403	TC	402:403	TC	402:403	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	44	dep	nutrients	342:350	arg1	P					376:376	P	376:376	P	376:376	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	44	dep	nutrients	342:350	arg1	N-NH4					386:390	N-NH4	386:390	N-NH4	386:390	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	44	dep	nutrients	342:350	arg1	nutrients					342:350	different nutrients	332:350	different nutrients	332:350	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	44	dep	nutrients	342:350	arg1	SO4					393:395	SO4	393:395	SO4	393:395	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	44	dep	nutrients	342:350	arg1	PO4					409:411	PO4	409:411	PO4	409:411	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	44	dep	nutrients	342:350	arg1	Zn					379:380	Zn	379:380	Zn	379:380	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	2	45	theme	adsorption	473:482	arg1	studies					484:490	Batch adsorption studies	467:490	Batch adsorption studies	467:490	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	4	46	theme	%	1074:1074	arg1	waste					1104:1108	0.5% cellulosic vineyard pruning waste	1071:1108	0.5% cellulosic vineyard pruning waste	1071:1108	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	1	47	theme	sugars	222:227	arg1	free					199:202	free	199:202	free	199:202	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	0	48	theme	pruning	36:42	arg1	composite					50:58	an alginate-vineyard pruning waste composite	15:58	an alginate-vineyard pruning waste composite	15:58	Formulation of an alginate-vineyard pruning waste composite as a new eco-friendly adsorbent to remove micronutrients from agroindustrial effluents.
24997896	2	49	theme	Batch	467:471	arg1	studies					484:490	Batch adsorption studies	467:490	Batch adsorption studies	467:490	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	4	50	theme	pruning	1096:1102	arg1	waste					1104:1108	0.5% cellulosic vineyard pruning waste	1071:1108	0.5% cellulosic vineyard pruning waste	1071:1108	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	3	51	theme	adsorbent	681:689	arg1	composite					691:699	the adsorbent composite	677:699	the adsorbent composite	677:699	The optimal formulation of the adsorbent composite varied depending on the target contaminant.
24997896	4	52	dep	comprised	839:847	arg1	whereas					936:942	whereas	936:942	whereas	936:942	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	53	theme	vineyard	1087:1094	arg1	waste					1104:1108	0.5% cellulosic vineyard pruning waste	1071:1108	0.5% cellulosic vineyard pruning waste	1071:1108	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	1	54	theme	agroindustrial	422:435	arg1	effluent					437:444	an agroindustrial effluent	419:444	an agroindustrial effluent (winery wastewater)	419:464	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	54	theme	agroindustrial	422:435	arg1	wastewater					454:463	winery wastewater	447:463	winery wastewater	447:463	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	0	55	theme	composite	50:58	arg1	Formulation					0:10	Formulation	0:10	Formulation of an alginate-vineyard pruning waste composite as a new eco-friendly adsorbent to remove micronutrients from agroindustrial effluents.	0:146	Formulation of an alginate-vineyard pruning waste composite as a new eco-friendly adsorbent to remove micronutrients from agroindustrial effluents.
24997896	1	56	dep	waste	192:196	arg1	free					199:202	free	199:202	free	199:202	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	2	57	theme	chloride	599:606	arg1	adsorbent					544:552	adsorbent	544:552	adsorbent	544:552	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	57	theme	chloride	599:606	arg1	alginate					571:578	sodium alginate	564:578	sodium alginate (1-5%)	564:585	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	57	theme	chloride	599:606	arg1	chloride					599:606	calcium chloride	591:606	calcium chloride (0.05-0.9M)	591:618	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	57	theme	chloride	599:606	arg1	0.05-0.9M					609:617	0.05-0.9M	609:617	0.05-0.9M	609:617	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	57	theme	chloride	599:606	arg1	%					560:560	0.5-2%	555:560	0.5-2%	555:560	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	57	theme	chloride	599:606	arg1	%					584:584	1-5%	581:584	1-5%	581:584	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	57	theme	chloride	599:606	arg1	amounts					522:528	the amounts	518:528	the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite	518:647	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	4	58	theme	pruning	921:927	arg1	waste					929:933	0.5% cellulosic vineyard pruning waste	896:933	0.5% cellulosic vineyard pruning waste	896:933	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	59	theme	calcium	1050:1056	arg1	chloride					1058:1065	0.9M calcium chloride	1045:1065	0.9M calcium chloride	1045:1065	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	2	60	theme	calcium	591:597	arg1	0.05-0.9M					609:617	0.05-0.9M	609:617	0.05-0.9M	609:617	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	2	60	theme	calcium	591:597	arg1	chloride					599:606	calcium chloride	591:606	calcium chloride (0.05-0.9M)	591:618	Batch adsorption studies were performed by varying the amounts of cellulosic adsorbent (0.5-2%), sodium alginate (1-5%) and calcium chloride (0.05-0.9M) included in the biocomposite.
24997896	4	61	theme	optimal	999:1005	arg1	mixture					1007:1013	the optimal mixture	995:1013	the optimal mixture	995:1013	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	62	theme	0.5	1071:1073	arg1	%					1074:1074	%	1074:1074	%	1074:1074	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	5	63	dep	%	1236:1236	arg1	waste					1266:1270	cellulosic vineyard pruning waste	1238:1270	0.5% cellulosic vineyard pruning waste	1233:1270	To remove TC from the winery wastewater, the optimal mixture comprised 3% of sodium alginate, 0.475M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	1	64	theme	winery	447:452	arg1	effluent					437:444	an agroindustrial effluent	419:444	an agroindustrial effluent (winery wastewater)	419:464	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	64	theme	winery	447:452	arg1	wastewater					454:463	winery wastewater	447:463	winery wastewater	447:463	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	0	65	theme	new	65:67	arg1	eco-friendly					69:80	a new eco-friendly	63:80	a new eco-friendly adsorbent to remove micronutrients from agroindustrial effluents	63:145	Formulation of an alginate-vineyard pruning waste composite as a new eco-friendly adsorbent to remove micronutrients from agroindustrial effluents.
24997896	1	66	theme	cellulosic	152:161	arg1	fraction					163:170	The cellulosic fraction	148:170	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars)	148:228	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	66	theme	cellulosic	152:161	arg1	adsorbent					303:311	adsorbent	303:311	adsorbent	303:311	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	4	67	theme	vineyard	912:919	arg1	waste					929:933	0.5% cellulosic vineyard pruning waste	896:933	0.5% cellulosic vineyard pruning waste	896:933	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	3	68	theme	composite	691:699	arg1	formulation					662:672	The optimal formulation	650:672	The optimal formulation of the adsorbent composite	650:699	The optimal formulation of the adsorbent composite varied depending on the target contaminant.
24997896	1	69	theme	different	332:340	arg1	K					383:383	K	383:383	K	383:383	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	69	theme	different	332:340	arg1	TN					398:399	TN	398:399	TN	398:399	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	69	theme	different	332:340	arg1	Mg					372:373	Mg	372:373	Mg	372:373	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	69	theme	different	332:340	arg1	TC					402:403	TC	402:403	TC	402:403	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	69	theme	different	332:340	arg1	P					376:376	P	376:376	P	376:376	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	69	theme	different	332:340	arg1	N-NH4					386:390	N-NH4	386:390	N-NH4	386:390	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	69	theme	different	332:340	arg1	nutrients					342:350	different nutrients	332:350	different nutrients	332:350	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	69	theme	different	332:340	arg1	SO4					393:395	SO4	393:395	SO4	393:395	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	69	theme	different	332:340	arg1	PO4					409:411	PO4	409:411	PO4	409:411	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	1	69	theme	different	332:340	arg1	Zn					379:380	Zn	379:380	Zn	379:380	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
24997896	4	70	dep	compounds	967:975	arg1	P					978:978	P	978:978	P	978:978	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	71	theme	sodium	1028:1033	arg1	alginate					1035:1042	1% sodium alginate	1025:1042	1% sodium alginate	1025:1042	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	4	72	theme	0.5	896:898	arg1	%					899:899	%	899:899	%	899:899	Thus, for the adsorption of cationic contaminants (Mg, Zn, K, N-NH4 and TN), the best mixture comprised 5% sodium alginate, 0.05M calcium chloride and 0.5% cellulosic vineyard pruning waste, whereas for removal of anionic compounds (P, SO4 and PO4), the optimal mixture comprised 1% sodium alginate, 0.9M calcium chloride and 0.5% cellulosic vineyard pruning waste.
24997896	1	73	theme	calcium	247:253	arg1	beads					264:268	calcium alginate beads	247:268	calcium alginate beads	247:268	The cellulosic fraction of vineyard pruning waste (free of hemicellulosic sugars) was entrapped in calcium alginate beads and evaluated as an eco-friendly adsorbent for the removal of different nutrients and micronutrients (Mg, P, Zn, K, N-NH4, SO4, TN, TC and PO4) from an agroindustrial effluent (winery wastewater).
26353385	7	0	theme	cardiolipin	1471:1481	arg1	species					1483:1489	linoleic acid-rich cardiolipin species	1452:1489	linoleic acid-rich cardiolipin species	1452:1489	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	6	1	theme	cardiolipin	1255:1265	arg1	compositions					1267:1278	cardiolipin compositions	1255:1278	cardiolipin compositions	1255:1278	Our results suggest that both intra- and extramitochondrial H2O2 are involved in cytochrome c release, but the persisting difference between C and SF levels can be attributed to the differences in cardiolipin compositions.
26353385	7	2	theme	linoleic	1452:1459	arg1	species					1483:1489	linoleic acid-rich cardiolipin species	1452:1489	linoleic acid-rich cardiolipin species	1452:1489	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	8	3	theme	SF	1617:1618	arg1	mitochondria					1620:1631	SF mitochondria	1617:1631	SF mitochondria	1617:1631	In addition, the content of tafazzin, a protein responsible for cardiolipin remodeling, was decreased in SF mitochondria.
26353385	4	4	theme	mitochondria	639:650	arg1	Exposure					627:634	Exposure	627:634	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system	627:732	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	2	5	theme	ROS	257:259	arg1	generation					261:270	ROS generation	257:270	ROS generation	257:270	With ROS generation being increased in fatty liver from sucrose-fed (SF) rats, we hypothesized that cytochrome c release might be positively associated with H2O2 generation from SF mitochondria.
26353385	2	6	from	rats	325:328	arg1	liver					297:301	fatty liver	291:301	fatty liver from sucrose-fed (SF) rats	291:328	With ROS generation being increased in fatty liver from sucrose-fed (SF) rats, we hypothesized that cytochrome c release might be positively associated with H2O2 generation from SF mitochondria.
26353385	6	7	theme	c	1150:1150	arg1	release					1152:1158	cytochrome c release	1139:1158	cytochrome c release	1139:1158	Our results suggest that both intra- and extramitochondrial H2O2 are involved in cytochrome c release, but the persisting difference between C and SF levels can be attributed to the differences in cardiolipin compositions.
26353385	4	8	theme	exogenous	655:663	arg1	radical					676:682	exogenous superoxide radical	655:682	exogenous superoxide radical generated by the xanthine/xanthine oxidase system	655:732	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	7	9	theme	lipid	1370:1374	arg1	membrane					1376:1383	lipid membrane	1370:1383	lipid membrane from SF mitochondria	1370:1404	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	0	10	theme	sucrose	118:124	arg1	feeding					126:132	sucrose feeding	118:132	sucrose feeding	118:132	Cytochrome c release from rat liver mitochondria is compromised by increased saturated cardiolipin species induced by sucrose feeding.
26353385	3	11	theme	C	571:571	arg1	mitochondria					574:585	control (C) mitochondria	562:585	control (C) mitochondria oxidizing pyruvate/malate or succinate	562:624	Surprisingly, cytochrome c release from mitochondria of SF liver was found to be significantly lower compared with control (C) mitochondria oxidizing pyruvate/malate or succinate.
26353385	1	12	theme	reactive	210:217	arg1	ROS					235:237	ROS	235:237	ROS	235:237	Cytochrome c release from mitochondria has been described to be related to reactive oxygen species (ROS) generation.
26353385	1	12	theme	reactive	210:217	arg1	species					226:232	reactive oxygen species	210:232	reactive oxygen species (ROS) generation	210:249	Cytochrome c release from mitochondria has been described to be related to reactive oxygen species (ROS) generation.
26353385	6	13	theme	SF	1205:1206	arg1	levels					1208:1213	SF levels	1205:1213	SF levels	1205:1213	Our results suggest that both intra- and extramitochondrial H2O2 are involved in cytochrome c release, but the persisting difference between C and SF levels can be attributed to the differences in cardiolipin compositions.
26353385	4	14	theme	xanthine/xanthine	701:717	arg1	system					727:732	the xanthine/xanthine oxidase system	697:732	the xanthine/xanthine oxidase system	697:732	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	3	15	theme	control	562:568	arg1	mitochondria					574:585	control (C) mitochondria	562:585	control (C) mitochondria oxidizing pyruvate/malate or succinate	562:624	Surprisingly, cytochrome c release from mitochondria of SF liver was found to be significantly lower compared with control (C) mitochondria oxidizing pyruvate/malate or succinate.
26353385	9	16	theme	cardiolipin	1705:1715	arg1	species					1727:1733	cardiolipin molecular species	1705:1733	cardiolipin molecular species in SF mitochondria	1705:1752	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	1	17	theme	Cytochrome	135:144	arg1	release					148:154	Cytochrome c release	135:154	Cytochrome c release from mitochondria	135:172	Cytochrome c release from mitochondria has been described to be related to reactive oxygen species (ROS) generation.
26353385	9	18	theme	species	1727:1733	arg1	composition					1690:1700	the composition	1686:1700	the composition of cardiolipin molecular species in SF mitochondria	1686:1752	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	7	19	theme	cardiolipin	1321:1331	arg1	species					1333:1339	palmitic acid-rich cardiolipin species	1302:1339	palmitic acid-rich cardiolipin species	1302:1339	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	7	20	from	mitochondria	1393:1404	arg1	membrane					1376:1383	lipid membrane	1370:1383	lipid membrane from SF mitochondria	1370:1404	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	9	21	theme	SF	1738:1739	arg1	mitochondria					1741:1752	SF mitochondria	1738:1752	SF mitochondria	1738:1752	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	0	22	theme	increased	67:75	arg1	species					99:105	increased saturated cardiolipin species	67:105	increased saturated cardiolipin species induced by sucrose feeding	67:132	Cytochrome c release from rat liver mitochondria is compromised by increased saturated cardiolipin species induced by sucrose feeding.
26353385	0	23	theme	cardiolipin	87:97	arg1	species					99:105	increased saturated cardiolipin species	67:105	increased saturated cardiolipin species induced by sucrose feeding	67:132	Cytochrome c release from rat liver mitochondria is compromised by increased saturated cardiolipin species induced by sucrose feeding.
26353385	9	24	from	mitochondria	1873:1884	arg1	release					1857:1863	its reduced release	1845:1863	its reduced release from SF mitochondria	1845:1884	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	5	25	theme	catalase	959:966	arg1	addition					921:928	the addition	917:928	the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger,	917:985	Furthermore, the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger, significantly reduces cytochrome c release from C and SF mitochondria.
26353385	0	26	from	mitochondria	36:47	arg1	release					13:19	Cytochrome c release	0:19	Cytochrome c release from rat liver mitochondria	0:47	Cytochrome c release from rat liver mitochondria is compromised by increased saturated cardiolipin species induced by sucrose feeding.
26353385	7	27	theme	acid-rich	1311:1319	arg1	species					1333:1339	palmitic acid-rich cardiolipin species	1302:1339	palmitic acid-rich cardiolipin species	1302:1339	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	9	28	theme	cytochrome	1773:1782	arg1	c					1784:1784	cytochrome c	1773:1784	cytochrome c interaction with mitochondrial inner membrane lipid	1773:1836	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	2	29	theme	cytochrome	352:361	arg1	release					365:371	cytochrome c release	352:371	cytochrome c release	352:371	With ROS generation being increased in fatty liver from sucrose-fed (SF) rats, we hypothesized that cytochrome c release might be positively associated with H2O2 generation from SF mitochondria.
26353385	0	30	theme	Cytochrome	0:9	arg1	release					13:19	Cytochrome c release	0:19	Cytochrome c release from rat liver mitochondria	0:47	Cytochrome c release from rat liver mitochondria is compromised by increased saturated cardiolipin species induced by sucrose feeding.
26353385	2	31	theme	H2O2	409:412	arg1	generation					414:423	H2O2 generation	409:423	H2O2 generation from SF mitochondria	409:444	With ROS generation being increased in fatty liver from sucrose-fed (SF) rats, we hypothesized that cytochrome c release might be positively associated with H2O2 generation from SF mitochondria.
26353385	4	32	theme	cytochrome	758:767	arg1	release					771:777	a dose-response cytochrome c release	742:777	a dose-response cytochrome c release	742:777	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	9	33	theme	mitochondrial	1803:1815	arg1	lipid					1832:1836	mitochondrial inner membrane lipid	1803:1836	mitochondrial inner membrane lipid	1803:1836	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	0	34	theme	rat	26:28	arg1	mitochondria					36:47	rat liver mitochondria	26:47	rat liver mitochondria	26:47	Cytochrome c release from rat liver mitochondria is compromised by increased saturated cardiolipin species induced by sucrose feeding.
26353385	5	35	theme	cytochrome	1009:1018	arg1	release					1022:1028	cytochrome c release	1009:1028	cytochrome c release from C and SF mitochondria	1009:1055	Furthermore, the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger, significantly reduces cytochrome c release from C and SF mitochondria.
26353385	9	36	theme	membrane	1823:1830	arg1	lipid					1832:1836	mitochondrial inner membrane lipid	1803:1836	mitochondrial inner membrane lipid	1803:1836	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	4	37	theme	c	831:831	arg1	release					833:839	cytochrome c release	820:839	cytochrome c release	820:839	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	3	38	theme	c	472:472	arg1	release					474:480	cytochrome c release	461:480	cytochrome c release from mitochondria of SF liver	461:510	Surprisingly, cytochrome c release from mitochondria of SF liver was found to be significantly lower compared with control (C) mitochondria oxidizing pyruvate/malate or succinate.
26353385	6	39	theme	persisting	1169:1178	arg1	difference					1180:1189	the persisting difference	1165:1189	the persisting difference between C and SF levels	1165:1213	Our results suggest that both intra- and extramitochondrial H2O2 are involved in cytochrome c release, but the persisting difference between C and SF levels can be attributed to the differences in cardiolipin compositions.
26353385	1	40	theme	oxygen	219:224	arg1	ROS					235:237	ROS	235:237	ROS	235:237	Cytochrome c release from mitochondria has been described to be related to reactive oxygen species (ROS) generation.
26353385	1	40	theme	oxygen	219:224	arg1	species					226:232	reactive oxygen species	210:232	reactive oxygen species (ROS) generation	210:249	Cytochrome c release from mitochondria has been described to be related to reactive oxygen species (ROS) generation.
26353385	9	41	from	species	1727:1733	arg1	mitochondria					1741:1752	SF mitochondria	1738:1752	SF mitochondria	1738:1752	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	7	42	theme	acid-rich	1461:1469	arg1	species					1483:1489	linoleic acid-rich cardiolipin species	1452:1489	linoleic acid-rich cardiolipin species	1452:1489	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	4	43	theme	SF	858:859	arg1	mitochondria					861:872	SF mitochondria	858:872	SF mitochondria	858:872	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	9	44	theme	SF	1870:1871	arg1	mitochondria					1873:1884	SF mitochondria	1870:1884	SF mitochondria	1870:1884	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	3	45	from	mitochondria	487:498	arg1	release					474:480	cytochrome c release	461:480	cytochrome c release from mitochondria of SF liver	461:510	Surprisingly, cytochrome c release from mitochondria of SF liver was found to be significantly lower compared with control (C) mitochondria oxidizing pyruvate/malate or succinate.
26353385	7	46	dep	found	1495:1499	arg1	decreased					1501:1509	decreased	1501:1509	was found decreased	1491:1509	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	7	47	theme	C	1420:1420	arg1	mitochondria					1422:1433	C mitochondria	1420:1433	C mitochondria	1420:1433	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	3	48	theme	liver	506:510	arg1	mitochondria					487:498	mitochondria	487:498	mitochondria of SF liver	487:510	Surprisingly, cytochrome c release from mitochondria of SF liver was found to be significantly lower compared with control (C) mitochondria oxidizing pyruvate/malate or succinate.
26353385	5	49	from	C	1035:1035	arg1	release					1022:1028	cytochrome c release	1009:1028	cytochrome c release from C and SF mitochondria	1009:1055	Furthermore, the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger, significantly reduces cytochrome c release from C and SF mitochondria.
26353385	9	50	with	interaction	1786:1796	arg1	lipid					1832:1836	mitochondrial inner membrane lipid	1803:1836	mitochondrial inner membrane lipid	1803:1836	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	7	51	dep	increased	1357:1365	arg1	compared					1406:1413	compared	1406:1413	compared with C mitochondria	1406:1433	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	6	52	theme	cytochrome	1139:1148	arg1	release					1152:1158	cytochrome c release	1139:1158	cytochrome c release	1139:1158	Our results suggest that both intra- and extramitochondrial H2O2 are involved in cytochrome c release, but the persisting difference between C and SF levels can be attributed to the differences in cardiolipin compositions.
26353385	2	53	theme	fatty	291:295	arg1	liver					297:301	fatty liver	291:301	fatty liver from sucrose-fed (SF) rats	291:328	With ROS generation being increased in fatty liver from sucrose-fed (SF) rats, we hypothesized that cytochrome c release might be positively associated with H2O2 generation from SF mitochondria.
26353385	7	54	theme	SF	1390:1391	arg1	mitochondria					1393:1404	SF mitochondria	1390:1404	SF mitochondria	1390:1404	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	9	55	located	observed	1674:1681	arg2	changes					1666:1672	the changes	1662:1672	the changes observed in the composition of cardiolipin molecular species in SF mitochondria	1662:1752	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	9	55	located	observed	1674:1681	arg1	composition					1690:1700	the composition	1686:1700	the composition of cardiolipin molecular species in SF mitochondria	1686:1752	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	4	56	theme	C	888:888	arg1	mitochondria					890:901	C mitochondria	888:901	C mitochondria	888:901	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	9	57	theme	molecular	1717:1725	arg1	species					1727:1733	cardiolipin molecular species	1705:1733	cardiolipin molecular species in SF mitochondria	1705:1752	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	4	58	theme	oxidase	719:725	arg1	system					727:732	the xanthine/xanthine oxidase system	697:732	the xanthine/xanthine oxidase system	697:732	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	5	59	theme	SF	1041:1042	arg1	mitochondria					1044:1055	SF mitochondria	1041:1055	SF mitochondria	1041:1055	Furthermore, the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger, significantly reduces cytochrome c release from C and SF mitochondria.
26353385	1	60	theme	c	146:146	arg1	release					148:154	Cytochrome c release	135:154	Cytochrome c release from mitochondria	135:172	Cytochrome c release from mitochondria has been described to be related to reactive oxygen species (ROS) generation.
26353385	9	61	from	composition	1690:1700	arg1	mitochondria					1741:1752	SF mitochondria	1738:1752	SF mitochondria	1738:1752	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	7	62	theme	species	1333:1339	arg1	ratio					1293:1297	the ratio	1289:1297	the ratio of palmitic acid-rich cardiolipin species	1289:1339	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	4	63	theme	superoxide	665:674	arg1	radical					676:682	exogenous superoxide radical	655:682	exogenous superoxide radical generated by the xanthine/xanthine oxidase system	655:732	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	0	64	theme	saturated	77:85	arg1	species					99:105	increased saturated cardiolipin species	67:105	increased saturated cardiolipin species induced by sucrose feeding	67:132	Cytochrome c release from rat liver mitochondria is compromised by increased saturated cardiolipin species induced by sucrose feeding.
26353385	1	65	from	mitochondria	161:172	arg1	release					148:154	Cytochrome c release	135:154	Cytochrome c release from mitochondria	135:172	Cytochrome c release from mitochondria has been described to be related to reactive oxygen species (ROS) generation.
26353385	2	66	from	mitochondria	433:444	arg1	generation					414:423	H2O2 generation	409:423	H2O2 generation from SF mitochondria	409:444	With ROS generation being increased in fatty liver from sucrose-fed (SF) rats, we hypothesized that cytochrome c release might be positively associated with H2O2 generation from SF mitochondria.
26353385	4	67	theme	c	769:769	arg1	release					771:777	a dose-response cytochrome c release	742:777	a dose-response cytochrome c release	742:777	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	3	68	theme	cytochrome	461:470	arg1	release					474:480	cytochrome c release	461:480	cytochrome c release from mitochondria of SF liver	461:510	Surprisingly, cytochrome c release from mitochondria of SF liver was found to be significantly lower compared with control (C) mitochondria oxidizing pyruvate/malate or succinate.
26353385	5	69	from	mitochondria	1044:1055	arg1	release					1022:1028	cytochrome c release	1009:1028	cytochrome c release from C and SF mitochondria	1009:1055	Furthermore, the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger, significantly reduces cytochrome c release from C and SF mitochondria.
26353385	7	70	theme	palmitic	1302:1309	arg1	species					1333:1339	palmitic acid-rich cardiolipin species	1302:1339	palmitic acid-rich cardiolipin species	1302:1339	Indeed, the ratio of palmitic acid-rich cardiolipin species was found to be increased in lipid membrane from SF mitochondria compared with C mitochondria, whereas that of linoleic acid-rich cardiolipin species was found decreased.
26353385	1	71	theme	species	226:232	arg1	generation					240:249	reactive oxygen species (ROS) generation	210:249	reactive oxygen species (ROS) generation	210:249	Cytochrome c release from mitochondria has been described to be related to reactive oxygen species (ROS) generation.
26353385	2	72	theme	c	363:363	arg1	release					365:371	cytochrome c release	352:371	cytochrome c release	352:371	With ROS generation being increased in fatty liver from sucrose-fed (SF) rats, we hypothesized that cytochrome c release might be positively associated with H2O2 generation from SF mitochondria.
26353385	0	73	theme	c	11:11	arg1	release					13:19	Cytochrome c release	0:19	Cytochrome c release from rat liver mitochondria	0:47	Cytochrome c release from rat liver mitochondria is compromised by increased saturated cardiolipin species induced by sucrose feeding.
26353385	5	74	theme	ebselen	933:939	arg1	addition					921:928	the addition	917:928	the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger,	917:985	Furthermore, the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger, significantly reduces cytochrome c release from C and SF mitochondria.
26353385	9	75	theme	c	1784:1784	arg1	interaction					1786:1796	cytochrome c interaction	1773:1796	cytochrome c interaction with mitochondrial inner membrane lipid	1773:1836	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	5	76	theme	PEG-catalase	942:953	arg1	addition					921:928	the addition	917:928	the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger,	917:985	Furthermore, the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger, significantly reduces cytochrome c release from C and SF mitochondria.
26353385	8	77	theme	tafazzin	1540:1547	arg1	content					1529:1535	the content	1525:1535	the content of tafazzin, a protein responsible for cardiolipin remodeling,	1525:1598	In addition, the content of tafazzin, a protein responsible for cardiolipin remodeling, was decreased in SF mitochondria.
26353385	4	78	theme	dose-response	744:756	arg1	release					771:777	a dose-response cytochrome c release	742:777	a dose-response cytochrome c release	742:777	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	0	79	theme	liver	30:34	arg1	mitochondria					36:47	rat liver mitochondria	26:47	rat liver mitochondria	26:47	Cytochrome c release from rat liver mitochondria is compromised by increased saturated cardiolipin species induced by sucrose feeding.
26353385	9	80	theme	inner	1817:1821	arg1	lipid					1832:1836	mitochondrial inner membrane lipid	1803:1836	mitochondrial inner membrane lipid	1803:1836	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	5	81	theme	c	1020:1020	arg1	release					1022:1028	cytochrome c release	1009:1028	cytochrome c release from C and SF mitochondria	1009:1055	Furthermore, the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger, significantly reduces cytochrome c release from C and SF mitochondria.
26353385	2	82	theme	SF	430:431	arg1	mitochondria					433:444	SF mitochondria	430:444	SF mitochondria	430:444	With ROS generation being increased in fatty liver from sucrose-fed (SF) rats, we hypothesized that cytochrome c release might be positively associated with H2O2 generation from SF mitochondria.
26353385	8	83	theme	responsible	1560:1570	arg1	tafazzin					1540:1547	tafazzin	1540:1547	tafazzin	1540:1547	In addition, the content of tafazzin, a protein responsible for cardiolipin remodeling, was decreased in SF mitochondria.
26353385	8	83	theme	responsible	1560:1570	arg1	protein					1552:1558	a protein	1550:1558	a protein responsible for cardiolipin remodeling	1550:1597	In addition, the content of tafazzin, a protein responsible for cardiolipin remodeling, was decreased in SF mitochondria.
26353385	4	84	theme	cytochrome	820:829	arg1	release					833:839	cytochrome c release	820:839	cytochrome c release	820:839	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	9	85	from	mitochondria	1741:1752	arg1	composition					1690:1700	the composition	1686:1700	the composition of cardiolipin molecular species in SF mitochondria	1686:1752	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	5	86	theme	H2O2	971:974	arg1	catalase					959:966	catalase	959:966	catalase	959:966	Furthermore, the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger, significantly reduces cytochrome c release from C and SF mitochondria.
26353385	5	86	theme	H2O2	971:974	arg1	scavenger					976:984	a H2O2 scavenger	969:984	a H2O2 scavenger	969:984	Furthermore, the addition of ebselen, PEG-catalase, or catalase, a H2O2 scavenger, significantly reduces cytochrome c release from C and SF mitochondria.
26353385	6	87	theme	extramitochondrial	1099:1116	arg1	H2O2					1118:1121	both intra- and extramitochondrial H2O2	1083:1121	both intra- and extramitochondrial H2O2	1083:1121	Our results suggest that both intra- and extramitochondrial H2O2 are involved in cytochrome c release, but the persisting difference between C and SF levels can be attributed to the differences in cardiolipin compositions.
26353385	8	88	theme	cardiolipin	1576:1586	arg1	remodeling					1588:1597	cardiolipin remodeling	1576:1597	cardiolipin remodeling	1576:1597	In addition, the content of tafazzin, a protein responsible for cardiolipin remodeling, was decreased in SF mitochondria.
26353385	2	89	theme	sucrose-fed	308:318	arg1	SF					321:322	SF	321:322	SF	321:322	With ROS generation being increased in fatty liver from sucrose-fed (SF) rats, we hypothesized that cytochrome c release might be positively associated with H2O2 generation from SF mitochondria.
26353385	2	89	theme	sucrose-fed	308:318	arg1	rats					325:328	sucrose-fed (SF) rats	308:328	sucrose-fed (SF) rats	308:328	With ROS generation being increased in fatty liver from sucrose-fed (SF) rats, we hypothesized that cytochrome c release might be positively associated with H2O2 generation from SF mitochondria.
26353385	6	90	from	differences	1240:1250	arg1	compositions					1267:1278	cardiolipin compositions	1255:1278	cardiolipin compositions	1255:1278	Our results suggest that both intra- and extramitochondrial H2O2 are involved in cytochrome c release, but the persisting difference between C and SF levels can be attributed to the differences in cardiolipin compositions.
26353385	6	91	theme	intra-	1088:1093	arg1	H2O2					1118:1121	both intra- and extramitochondrial H2O2	1083:1121	both intra- and extramitochondrial H2O2	1083:1121	Our results suggest that both intra- and extramitochondrial H2O2 are involved in cytochrome c release, but the persisting difference between C and SF levels can be attributed to the differences in cardiolipin compositions.
26353385	9	92	theme	reduced	1849:1855	arg1	release					1857:1863	its reduced release	1845:1863	its reduced release from SF mitochondria	1845:1884	Therefore, we conclude that the changes observed in the composition of cardiolipin molecular species in SF mitochondria may be involved in cytochrome c interaction with mitochondrial inner membrane lipid and in its reduced release from SF mitochondria.
26353385	4	93	theme	SF	799:800	arg1	mitochondria					802:813	SF mitochondria	799:813	SF mitochondria	799:813	Exposure of mitochondria to exogenous superoxide radical generated by the xanthine/xanthine oxidase system elicits a dose-response cytochrome c release in both control and SF mitochondria, but cytochrome c release remains lower in SF mitochondria compared with C mitochondria.
26353385	3	94	theme	SF	503:504	arg1	liver					506:510	SF liver	503:510	SF liver	503:510	Surprisingly, cytochrome c release from mitochondria of SF liver was found to be significantly lower compared with control (C) mitochondria oxidizing pyruvate/malate or succinate.
27695962	5	0	gly	glycoproteins	905:917	arg1	glycoproteins					905:917	192 glycoproteins	901:917	192 glycoproteins	901:917	Using an Orbitrap Fusion Tribrid mass spectrometer, we identified 699 intact glycopeptides from 192 glycoproteins.
27695962	4	1	theme	new	733:735	arg1	probes					737:742	new probes	733:742	new probes	733:742	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
27695962	3	2	gly	glycopeptides	597:609	arg2	glycopeptides					597:609	azide-labeled intact glycopeptides	576:609	azide-labeled intact glycopeptides from complex proteomes	576:632	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	3	2	gly	glycopeptides	597:609	arg1	proteomes					624:632	complex proteomes	616:632	complex proteomes	616:632	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	1	3	theme	Protein	150:156	arg1	modification					196:207	a post-translational modification	175:207	a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation	175:291	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	1	3	theme	Protein	150:156	arg1	glycosylation					158:170	Protein glycosylation	150:170	Protein glycosylation	150:170	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	1	4	theme	proteomic	247:255	arg1	diversity					257:265	proteomic diversity	247:265	proteomic diversity	247:265	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	0	5	theme	O-glycopeptides	72:86	arg1	analysis					42:49	Isotope-targeted glycoproteomics (IsoTaG) analysis	0:49	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid	0:116	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	3	6	theme	mass	535:538	arg1	glycoproteomics					488:502	isotope-targeted glycoproteomics	471:502	isotope-targeted glycoproteomics (IsoTaG)	471:511	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	3	6	theme	mass	535:538	arg1	method					553:558	a mass-independent mass spectrometry method	516:558	a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes	516:632	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	1	7	theme	diversity	257:265	arg1	aspects					236:242	many aspects	231:242	many aspects of proteomic diversity and biological regulation	231:291	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	0	8	theme	Orbitrap	94:101	arg1	Tribrid					110:116	an Orbitrap Fusion Tribrid	91:116	an Orbitrap Fusion Tribrid	91:116	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	6	9	gly	sialylated	974:983	arg1	structures					992:1001	eight sialylated glycan structures	968:1001	eight sialylated glycan structures across 126 N- and 576 O-glycopeptides	968:1039	These intact glycopeptides represent a total of eight sialylated glycan structures across 126 N- and 576 O-glycopeptides.
27695962	3	10	theme	azide-labeled	576:588	arg1	glycopeptides					597:609	azide-labeled intact glycopeptides	576:609	azide-labeled intact glycopeptides from complex proteomes	576:632	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	4	11	theme	glycoproteome	774:786	arg1	analysis					747:754	analysis	747:754	analysis of the sialylated glycoproteome from PC-3 cells	747:802	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
27695962	4	12	gly	sialylated	763:772	arg1	glycoproteome					774:786	the sialylated glycoproteome	759:786	the sialylated glycoproteome from PC-3 cells	759:802	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
27695962	3	13	theme	intact	590:595	arg1	glycopeptides					597:609	azide-labeled intact glycopeptides	576:609	azide-labeled intact glycopeptides from complex proteomes	576:632	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	4	14	from	cells	798:802	arg1	glycoproteome					774:786	the sialylated glycoproteome	759:786	the sialylated glycoproteome from PC-3 cells	759:802	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
27695962	4	14	from	cells	798:802	arg1	analysis					747:754	analysis	747:754	analysis of the sialylated glycoproteome from PC-3 cells	747:802	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
27695962	3	15	theme	mass-independent	518:533	arg1	glycoproteomics					488:502	isotope-targeted glycoproteomics	471:502	isotope-targeted glycoproteomics (IsoTaG)	471:511	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	3	15	theme	mass-independent	518:533	arg1	method					553:558	a mass-independent mass spectrometry method	516:558	a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes	516:632	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	1	16	theme	biological	271:280	arg1	regulation					282:291	biological regulation	271:291	biological regulation	271:291	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	4	17	theme	sialylated	763:772	arg1	glycoproteome					774:786	the sialylated glycoproteome	759:786	the sialylated glycoproteome from PC-3 cells	759:802	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
27695962	0	18	gly	O-glycopeptides	72:86	arg2	O-glycopeptides					72:86	O-glycopeptides	72:86	O-glycopeptides	72:86	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	4	19	theme	metabolic	705:713	arg1	labels					715:720	metabolic labels	705:720	metabolic labels	705:720	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
27695962	4	20	theme	sugars	695:700	arg1	use					680:682	the use	676:682	the use of alkynyl sugars as metabolic labels	676:720	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
27695962	0	21	theme	Fusion	103:108	arg1	Tribrid					110:116	an Orbitrap Fusion Tribrid	91:116	an Orbitrap Fusion Tribrid	91:116	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	0	22	from	analysis	42:49	arg1	Tribrid					110:116	an Orbitrap Fusion Tribrid	91:116	an Orbitrap Fusion Tribrid	91:116	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	5	23	theme	mass	838:841	arg1	spectrometer					843:854	an Orbitrap Fusion Tribrid mass spectrometer	811:854	an Orbitrap Fusion Tribrid mass spectrometer	811:854	Using an Orbitrap Fusion Tribrid mass spectrometer, we identified 699 intact glycopeptides from 192 glycoproteins.
27695962	4	24	theme	alkynyl	687:693	arg1	sugars					695:700	alkynyl sugars	687:700	alkynyl sugars	687:700	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
27695962	0	25	theme	glycoproteomics	17:31	arg1	analysis					42:49	Isotope-targeted glycoproteomics (IsoTaG) analysis	0:49	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid	0:116	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	5	26	gly	glycopeptides	882:894	arg1	glycoproteins					905:917	192 glycoproteins	901:917	192 glycoproteins	901:917	Using an Orbitrap Fusion Tribrid mass spectrometer, we identified 699 intact glycopeptides from 192 glycoproteins.
27695962	5	26	gly	glycopeptides	882:894	arg2	glycopeptides					882:894	699 intact glycopeptides	871:894	699 intact glycopeptides from 192 glycoproteins	871:917	Using an Orbitrap Fusion Tribrid mass spectrometer, we identified 699 intact glycopeptides from 192 glycoproteins.
27695962	3	27	theme	complex	616:622	arg1	proteomes					624:632	complex proteomes	616:632	complex proteomes	616:632	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	1	28	theme	post-translational	177:194	arg1	PTM					210:212	PTM	210:212	PTM	210:212	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	1	28	theme	post-translational	177:194	arg1	modification					196:207	a post-translational modification	175:207	a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation	175:291	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	1	28	theme	post-translational	177:194	arg1	glycosylation					158:170	Protein glycosylation	150:170	Protein glycosylation	150:170	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	0	29	theme	Isotope-targeted	0:15	arg1	analysis					42:49	Isotope-targeted glycoproteomics (IsoTaG) analysis	0:49	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid	0:116	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	1	30	theme	regulation	282:291	arg1	aspects					236:242	many aspects	231:242	many aspects of proteomic diversity and biological regulation	231:291	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	7	31	theme	azido	1149:1153	arg1	sugars					1155:1160	azido sugars	1149:1160	azido sugars	1149:1160	IsoTaG is therefore an effective platform for identification of intact glycopeptides labeled by alkynyl or azido sugars and will facilitate further studies of the glycoproteome.
27695962	2	32	theme	structures	322:331	arg1	Assignment					294:303	Assignment	294:303	Assignment of intact glycan structures to specific protein attachment sites	294:368	Assignment of intact glycan structures to specific protein attachment sites is a critical step towards elucidating the function encoded in the glycome.
27695962	2	32	theme	structures	322:331	arg1	step					384:387	a critical step	373:387	a critical step towards elucidating the function encoded in the glycome	373:443	Assignment of intact glycan structures to specific protein attachment sites is a critical step towards elucidating the function encoded in the glycome.
27695962	0	33	theme	IsoTaG	34:39	arg1	analysis					42:49	Isotope-targeted glycoproteomics (IsoTaG) analysis	0:49	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid	0:116	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	0	34	theme	azido	124:128	arg1	sugars					142:147	azido and alkynyl sugars	124:147	sugars	142:147	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	2	35	theme	glycan	315:320	arg1	structures					322:331	intact glycan structures	308:331	intact glycan structures	308:331	Assignment of intact glycan structures to specific protein attachment sites is a critical step towards elucidating the function encoded in the glycome.
27695962	7	36	theme	glycopeptides	1113:1125	arg1	identification					1088:1101	identification	1088:1101	identification of intact glycopeptides labeled by alkynyl or azido sugars	1088:1160	IsoTaG is therefore an effective platform for identification of intact glycopeptides labeled by alkynyl or azido sugars and will facilitate further studies of the glycoproteome.
27695962	2	37	theme	intact	308:313	arg1	structures					322:331	intact glycan structures	308:331	intact glycan structures	308:331	Assignment of intact glycan structures to specific protein attachment sites is a critical step towards elucidating the function encoded in the glycome.
27695962	5	38	theme	192	901:903	arg1	glycoproteins					905:917	192 glycoproteins	901:917	192 glycoproteins	901:917	Using an Orbitrap Fusion Tribrid mass spectrometer, we identified 699 intact glycopeptides from 192 glycoproteins.
27695962	7	39	theme	glycoproteome	1205:1217	arg1	studies					1190:1196	further studies	1182:1196	further studies of the glycoproteome	1182:1217	IsoTaG is therefore an effective platform for identification of intact glycopeptides labeled by alkynyl or azido sugars and will facilitate further studies of the glycoproteome.
27695962	0	40	theme	alkynyl	134:140	arg1	sugars					142:147	azido and alkynyl sugars	124:147	sugars	142:147	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	0	41	from	O-glycopeptides	72:86	arg1	Tribrid					110:116	an Orbitrap Fusion Tribrid	91:116	an Orbitrap Fusion Tribrid	91:116	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	5	42	theme	intact	875:880	arg1	glycopeptides					882:894	699 intact glycopeptides	871:894	699 intact glycopeptides from 192 glycoproteins	871:917	Using an Orbitrap Fusion Tribrid mass spectrometer, we identified 699 intact glycopeptides from 192 glycoproteins.
27695962	5	43	theme	Orbitrap	814:821	arg1	spectrometer					843:854	an Orbitrap Fusion Tribrid mass spectrometer	811:854	an Orbitrap Fusion Tribrid mass spectrometer	811:854	Using an Orbitrap Fusion Tribrid mass spectrometer, we identified 699 intact glycopeptides from 192 glycoproteins.
27695962	7	44	theme	further	1182:1188	arg1	studies					1190:1196	further studies	1182:1196	further studies of the glycoproteome	1182:1217	IsoTaG is therefore an effective platform for identification of intact glycopeptides labeled by alkynyl or azido sugars and will facilitate further studies of the glycoproteome.
27695962	4	45	theme	PC-3	793:796	arg1	cells					798:802	PC-3 cells	793:802	PC-3 cells	793:802	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
27695962	7	46	theme	effective	1065:1073	arg1	IsoTaG					1042:1047	IsoTaG	1042:1047	IsoTaG	1042:1047	IsoTaG is therefore an effective platform for identification of intact glycopeptides labeled by alkynyl or azido sugars and will facilitate further studies of the glycoproteome.
27695962	7	46	theme	effective	1065:1073	arg1	platform					1075:1082	an effective platform	1062:1082	an effective platform for identification of intact glycopeptides labeled by alkynyl or azido sugars	1062:1160	IsoTaG is therefore an effective platform for identification of intact glycopeptides labeled by alkynyl or azido sugars and will facilitate further studies of the glycoproteome.
27695962	1	47	theme	responsible	215:225	arg1	PTM					210:212	PTM	210:212	PTM	210:212	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	1	47	theme	responsible	215:225	arg1	modification					196:207	a post-translational modification	175:207	a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation	175:291	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	1	47	theme	responsible	215:225	arg1	glycosylation					158:170	Protein glycosylation	150:170	Protein glycosylation	150:170	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	0	48	theme	sialylated	54:63	arg1	N-					65:66	N-	65:66	N-	65:66	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	6	49	theme	structures	992:1001	arg1	total					959:963	a total	957:963	a total of eight sialylated glycan structures across 126 N- and 576 O-glycopeptides	957:1039	These intact glycopeptides represent a total of eight sialylated glycan structures across 126 N- and 576 O-glycopeptides.
27695962	0	50	from	N-	65:66	arg1	Tribrid					110:116	an Orbitrap Fusion Tribrid	91:116	an Orbitrap Fusion Tribrid	91:116	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	6	51	theme	intact	926:931	arg1	glycopeptides					933:945	These intact glycopeptides	920:945	These intact glycopeptides	920:945	These intact glycopeptides represent a total of eight sialylated glycan structures across 126 N- and 576 O-glycopeptides.
27695962	5	52	theme	Tribrid	830:836	arg1	spectrometer					843:854	an Orbitrap Fusion Tribrid mass spectrometer	811:854	an Orbitrap Fusion Tribrid mass spectrometer	811:854	Using an Orbitrap Fusion Tribrid mass spectrometer, we identified 699 intact glycopeptides from 192 glycoproteins.
27695962	6	53	theme	glycan	985:990	arg1	structures					992:1001	eight sialylated glycan structures	968:1001	eight sialylated glycan structures across 126 N- and 576 O-glycopeptides	968:1039	These intact glycopeptides represent a total of eight sialylated glycan structures across 126 N- and 576 O-glycopeptides.
27695962	7	54	theme	intact	1106:1111	arg1	glycopeptides					1113:1125	intact glycopeptides	1106:1125	intact glycopeptides labeled by alkynyl or azido sugars	1106:1160	IsoTaG is therefore an effective platform for identification of intact glycopeptides labeled by alkynyl or azido sugars and will facilitate further studies of the glycoproteome.
27695962	0	55	from	Tribrid	110:116	arg1	analysis					42:49	Isotope-targeted glycoproteomics (IsoTaG) analysis	0:49	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid	0:116	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	6	56	gly	O-glycopeptides	1025:1039	arg2	O-glycopeptides					1025:1039	576 O-glycopeptides	1021:1039	576 O-glycopeptides	1021:1039	These intact glycopeptides represent a total of eight sialylated glycan structures across 126 N- and 576 O-glycopeptides.
27695962	2	57	theme	attachment	353:362	arg1	sites					364:368	specific protein attachment sites	336:368	specific protein attachment sites	336:368	Assignment of intact glycan structures to specific protein attachment sites is a critical step towards elucidating the function encoded in the glycome.
27695962	6	58	gly	glycopeptides	933:945	arg2	glycopeptides					933:945	These intact glycopeptides	920:945	These intact glycopeptides	920:945	These intact glycopeptides represent a total of eight sialylated glycan structures across 126 N- and 576 O-glycopeptides.
27695962	6	59	theme	sialylated	974:983	arg1	structures					992:1001	eight sialylated glycan structures	968:1001	eight sialylated glycan structures across 126 N- and 576 O-glycopeptides	968:1039	These intact glycopeptides represent a total of eight sialylated glycan structures across 126 N- and 576 O-glycopeptides.
27695962	7	60	gly	glycopeptides	1113:1125	arg2	glycopeptides					1113:1125	intact glycopeptides	1106:1125	intact glycopeptides labeled by alkynyl or azido sugars	1106:1160	IsoTaG is therefore an effective platform for identification of intact glycopeptides labeled by alkynyl or azido sugars and will facilitate further studies of the glycoproteome.
27695962	5	61	theme	Fusion	823:828	arg1	spectrometer					843:854	an Orbitrap Fusion Tribrid mass spectrometer	811:854	an Orbitrap Fusion Tribrid mass spectrometer	811:854	Using an Orbitrap Fusion Tribrid mass spectrometer, we identified 699 intact glycopeptides from 192 glycoproteins.
27695962	2	62	theme	protein	345:351	arg1	sites					364:368	specific protein attachment sites	336:368	specific protein attachment sites	336:368	Assignment of intact glycan structures to specific protein attachment sites is a critical step towards elucidating the function encoded in the glycome.
27695962	4	63	from	analysis	747:754	arg1	cells					798:802	PC-3 cells	793:802	PC-3 cells	793:802	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
27695962	5	64	from	glycoproteins	905:917	arg1	glycopeptides					882:894	699 intact glycopeptides	871:894	699 intact glycopeptides from 192 glycoproteins	871:917	Using an Orbitrap Fusion Tribrid mass spectrometer, we identified 699 intact glycopeptides from 192 glycoproteins.
27695962	2	65	theme	specific	336:343	arg1	sites					364:368	specific protein attachment sites	336:368	specific protein attachment sites	336:368	Assignment of intact glycan structures to specific protein attachment sites is a critical step towards elucidating the function encoded in the glycome.
27695962	1	66	theme	many	231:234	arg1	aspects					236:242	many aspects	231:242	many aspects of proteomic diversity and biological regulation	231:291	Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation.
27695962	3	67	theme	isotope-targeted	471:486	arg1	IsoTaG					505:510	IsoTaG	505:510	IsoTaG	505:510	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	3	67	theme	isotope-targeted	471:486	arg1	glycoproteomics					488:502	isotope-targeted glycoproteomics	471:502	isotope-targeted glycoproteomics (IsoTaG)	471:511	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	3	67	theme	isotope-targeted	471:486	arg1	method					553:558	a mass-independent mass spectrometry method	516:558	a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes	516:632	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	3	68	theme	spectrometry	540:551	arg1	glycoproteomics					488:502	isotope-targeted glycoproteomics	471:502	isotope-targeted glycoproteomics (IsoTaG)	471:511	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	3	68	theme	spectrometry	540:551	arg1	method					553:558	a mass-independent mass spectrometry method	516:558	a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes	516:632	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	3	69	from	proteomes	624:632	arg1	glycopeptides					597:609	azide-labeled intact glycopeptides	576:609	azide-labeled intact glycopeptides from complex proteomes	576:632	Previously, we developed isotope-targeted glycoproteomics (IsoTaG) as a mass-independent mass spectrometry method to characterize azide-labeled intact glycopeptides from complex proteomes.
27695962	0	70	theme	N-	65:66	arg1	analysis					42:49	Isotope-targeted glycoproteomics (IsoTaG) analysis	0:49	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid	0:116	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	0	71	gly	sialylated	54:63	arg1	N-					65:66	N-	65:66	N-	65:66	Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
27695962	2	72	theme	critical	375:382	arg1	Assignment					294:303	Assignment	294:303	Assignment of intact glycan structures to specific protein attachment sites	294:368	Assignment of intact glycan structures to specific protein attachment sites is a critical step towards elucidating the function encoded in the glycome.
27695962	2	72	theme	critical	375:382	arg1	step					384:387	a critical step	373:387	a critical step towards elucidating the function encoded in the glycome	373:443	Assignment of intact glycan structures to specific protein attachment sites is a critical step towards elucidating the function encoded in the glycome.
27695962	4	73	theme	IsoTaG	655:660	arg1	approach					662:669	the IsoTaG approach	651:669	the IsoTaG approach	651:669	Here, we extend the IsoTaG approach with the use of alkynyl sugars as metabolic labels and employ new probes in analysis of the sialylated glycoproteome from PC-3 cells.
26076634	6	0	theme	>Ni	967:969	arg1	order					945:949	the order	941:949	the order of Cu(II)>Cd(II)>Ni(II)	941:973	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	6	1	theme	binary	831:836	arg1	system					838:843	binary system	831:843	binary system	831:843	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	8	2	theme	nitrogen	1216:1223	arg1	atoms					1225:1229	the nitrogen atoms	1212:1229	the nitrogen atoms of cross-linking CTS/REC	1212:1254	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	4	3	theme	kinetic	730:736	arg1	model					738:742	the pseudo-second-order kinetic model	706:742	the pseudo-second-order kinetic model	706:742	In single system, the equilibrium studies showed that the adsorption of Cd(II), Cu(II) and Ni(II) followed the Langmuir model and the pseudo-second-order kinetic model.
26076634	3	4	theme	Ni	516:517	arg1	microsphere					482:492	the microsphere	478:492	the microsphere of Cd(II), Cu(II) and Ni(II)	478:521	The adsorption behavior of the microsphere of Cd(II), Cu(II) and Ni(II) was investigated in single and binary metal systems.
26076634	8	5	theme	cross-linking	1234:1246	arg1	CTS/REC					1248:1254	cross-linking CTS/REC	1234:1254	cross-linking CTS/REC	1234:1254	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	4	6	theme	Langmuir	687:694	arg1	model					696:700	the Langmuir model	683:700	the Langmuir model	683:700	In single system, the equilibrium studies showed that the adsorption of Cd(II), Cu(II) and Ni(II) followed the Langmuir model and the pseudo-second-order kinetic model.
26076634	5	7	theme	ΔG	769:770	arg1	values					758:763	The negative values	745:763	The negative values of (ΔG)	745:771	The negative values of (ΔG) suggested that the adsorption process was spontaneous.
26076634	1	8	theme	CTS/REC	222:228	arg1	proportion					208:217	the proportion	204:217	the proportion of CTS/REC with 2:1, 3:1 and 4:1	204:250	Chitosan/rectorie (CTS/REC) nano-hybrid composite microsphere was prepared by changing the proportion of CTS/REC with 2:1, 3:1 and 4:1.
26076634	0	9	theme	Ni	33:34	arg1	ions					40:43	Cd(II), Cu(II) and Ni(II) ions	14:43	Cd(II), Cu(II) and Ni(II) ions	14:43	Adsorption of Cd(II), Cu(II) and Ni(II) ions by cross-linking chitosan/rectorite nano-hybrid composite microspheres.
26076634	7	10	theme	cross-linking	1061:1073	arg1	microspheres					1075:1086	cross-linking microspheres	1061:1086	cross-linking microspheres	1061:1086	The regeneration studies indicated that EDTA desorbed Cd(II), Cu(II) and Ni(II) from cross-linking microspheres better than HCl.
26076634	4	11	theme	Cu	656:657	arg1	adsorption					634:643	the adsorption	630:643	the adsorption of Cd(II), Cu(II) and Ni(II)	630:672	In single system, the equilibrium studies showed that the adsorption of Cd(II), Cu(II) and Ni(II) followed the Langmuir model and the pseudo-second-order kinetic model.
26076634	8	12	theme	nano-hybrid	1256:1266	arg1	microspheres					1278:1289	nano-hybrid composite microspheres	1256:1289	nano-hybrid composite microspheres	1256:1289	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	3	13	theme	single	543:548	arg1	systems					567:573	single and binary metal systems	543:573	single and binary metal systems	543:573	The adsorption behavior of the microsphere of Cd(II), Cu(II) and Ni(II) was investigated in single and binary metal systems.
26076634	4	14	theme	Cd	648:649	arg1	adsorption					634:643	the adsorption	630:643	the adsorption of Cd(II), Cu(II) and Ni(II)	630:672	In single system, the equilibrium studies showed that the adsorption of Cd(II), Cu(II) and Ni(II) followed the Langmuir model and the pseudo-second-order kinetic model.
26076634	2	15	theme	better	369:374	arg1	capacity					385:392	better sorption capacity	369:392	better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1)	369:448	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	8	16	theme	CTS/REC	1248:1254	arg1	atoms					1225:1229	the nitrogen atoms	1212:1229	the nitrogen atoms of cross-linking CTS/REC	1212:1254	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	8	16	theme	CTS/REC	1248:1254	arg1	II					1204:1205	II	1204:1205	II	1204:1205	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	8	16	theme	CTS/REC	1248:1254	arg1	Ni					1201:1202	Ni	1201:1202	Ni(II)	1201:1206	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	8	16	theme	CTS/REC	1248:1254	arg1	Cu					1190:1191	Cu(II)	1190:1195	Cu(II)	1190:1195	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	8	16	theme	CTS/REC	1248:1254	arg1	Cd					1182:1183	Cd(II)	1182:1187	Cd(II)	1182:1187	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	2	17	theme	Ni	416:417	arg1	capacity					385:392	better sorption capacity	369:392	better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1)	369:448	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	8	18	theme	coordination	1143:1154	arg1	bonds					1156:1160	coordination bonds	1143:1160	coordination bonds	1143:1160	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	6	19	theme	combined	850:857	arg1	antagonistic					896:907	antagonistic	896:907	antagonistic	896:907	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	6	19	theme	combined	850:857	arg1	action					859:864	the combined action	846:864	the combined action of the metals	846:878	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	4	20	theme	single	579:584	arg1	system					586:591	single system	579:591	single system	579:591	In single system, the equilibrium studies showed that the adsorption of Cd(II), Cu(II) and Ni(II) followed the Langmuir model and the pseudo-second-order kinetic model.
26076634	8	21	theme	composite	1268:1276	arg1	microspheres					1278:1289	nano-hybrid composite microspheres	1256:1289	nano-hybrid composite microspheres	1256:1289	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	2	22	theme	Cd	397:398	arg1	capacity					385:392	better sorption capacity	369:392	better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1)	369:448	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	3	23	theme	metal	561:565	arg1	systems					567:573	single and binary metal systems	543:573	single and binary metal systems	543:573	The adsorption behavior of the microsphere of Cd(II), Cu(II) and Ni(II) was investigated in single and binary metal systems.
26076634	6	24	theme	>Cd	960:962	arg1	>Ni					967:969	Cu(II)>Cd(II)>Ni(II)	954:973	Cu(II)>Cd(II)>Ni(II)	954:973	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	2	25	theme	composite	328:336	arg1	microsphere					338:348	the nano-hybrid composite microsphere	312:348	the nano-hybrid composite microsphere	312:348	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	5	26	theme	negative	749:756	arg1	values					758:763	The negative values	745:763	The negative values of (ΔG)	745:771	The negative values of (ΔG) suggested that the adsorption process was spontaneous.
26076634	6	27	theme	metals	873:878	arg1	antagonistic					896:907	antagonistic	896:907	antagonistic	896:907	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	6	27	theme	metals	873:878	arg1	action					859:864	the combined action	846:864	the combined action of the metals	846:878	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	2	28	theme	sorption	376:383	arg1	capacity					385:392	better sorption capacity	369:392	better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1)	369:448	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	0	29	theme	Cd	14:15	arg1	ions					40:43	Cd(II), Cu(II) and Ni(II) ions	14:43	Cd(II), Cu(II) and Ni(II) ions	14:43	Adsorption of Cd(II), Cu(II) and Ni(II) ions by cross-linking chitosan/rectorite nano-hybrid composite microspheres.
26076634	0	30	theme	ions	40:43	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of Cd(II), Cu(II) and Ni(II) ions by cross-linking	0:60	Adsorption of Cd(II), Cu(II) and Ni(II) ions by cross-linking chitosan/rectorite nano-hybrid composite microspheres.
26076634	6	31	theme	metal	917:921	arg1	sorption					923:930	the metal sorption	913:930	the metal sorption	913:930	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	1	32	theme	composite	157:165	arg1	microsphere					167:177	Chitosan/rectorie (CTS/REC) nano-hybrid composite microsphere	117:177	Chitosan/rectorie (CTS/REC) nano-hybrid composite microsphere	117:177	Chitosan/rectorie (CTS/REC) nano-hybrid composite microsphere was prepared by changing the proportion of CTS/REC with 2:1, 3:1 and 4:1.
26076634	2	33	theme	Cu	405:406	arg1	capacity					385:392	better sorption capacity	369:392	better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1)	369:448	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	3	34	theme	binary	554:559	arg1	systems					567:573	single and binary metal systems	543:573	single and binary metal systems	543:573	The adsorption behavior of the microsphere of Cd(II), Cu(II) and Ni(II) was investigated in single and binary metal systems.
26076634	3	35	theme	Cu	505:506	arg1	microsphere					482:492	the microsphere	478:492	the microsphere of Cd(II), Cu(II) and Ni(II)	478:521	The adsorption behavior of the microsphere of Cd(II), Cu(II) and Ni(II) was investigated in single and binary metal systems.
26076634	6	36	theme	Cu	954:955	arg1	>Ni					967:969	Cu(II)>Cd(II)>Ni(II)	954:973	Cu(II)>Cd(II)>Ni(II)	954:973	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	7	37	theme	regeneration	980:991	arg1	studies					993:999	The regeneration studies	976:999	The regeneration studies	976:999	The regeneration studies indicated that EDTA desorbed Cd(II), Cu(II) and Ni(II) from cross-linking microspheres better than HCl.
26076634	1	38	with	CTS/REC	222:228	arg1	3:1					240:242	3:1	240:242	3:1	240:242	Chitosan/rectorie (CTS/REC) nano-hybrid composite microsphere was prepared by changing the proportion of CTS/REC with 2:1, 3:1 and 4:1.
26076634	1	38	with	CTS/REC	222:228	arg1	2:1					235:237	2:1	235:237	2:1	235:237	Chitosan/rectorie (CTS/REC) nano-hybrid composite microsphere was prepared by changing the proportion of CTS/REC with 2:1, 3:1 and 4:1.
26076634	1	38	with	CTS/REC	222:228	arg1	4:1					248:250	4:1	248:250	4:1	248:250	Chitosan/rectorie (CTS/REC) nano-hybrid composite microsphere was prepared by changing the proportion of CTS/REC with 2:1, 3:1 and 4:1.
26076634	0	39	theme	Cu	22:23	arg1	ions					40:43	Cd(II), Cu(II) and Ni(II) ions	14:43	Cd(II), Cu(II) and Ni(II) ions	14:43	Adsorption of Cd(II), Cu(II) and Ni(II) ions by cross-linking chitosan/rectorite nano-hybrid composite microspheres.
26076634	0	40	theme	composite	93:101	arg1	microspheres					103:114	nano-hybrid composite microspheres	81:114	nano-hybrid composite microspheres	81:114	Adsorption of Cd(II), Cu(II) and Ni(II) ions by cross-linking chitosan/rectorite nano-hybrid composite microspheres.
26076634	8	41	dep	Cd	1182:1183	arg1	microspheres					1278:1289	nano-hybrid composite microspheres	1256:1289	nano-hybrid composite microspheres	1256:1289	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	3	42	theme	adsorption	455:464	arg1	behavior					466:473	The adsorption behavior	451:473	The adsorption behavior of the microsphere of Cd(II), Cu(II) and Ni(II)	451:521	The adsorption behavior of the microsphere of Cd(II), Cu(II) and Ni(II) was investigated in single and binary metal systems.
26076634	2	43	theme	chitosan	290:297	arg1	microsphere					299:309	the pure cross-linking chitosan microsphere	267:309	the pure cross-linking chitosan microsphere	267:309	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	0	44	theme	nano-hybrid	81:91	arg1	microspheres					103:114	nano-hybrid composite microspheres	81:114	nano-hybrid composite microspheres	81:114	Adsorption of Cd(II), Cu(II) and Ni(II) ions by cross-linking chitosan/rectorite nano-hybrid composite microspheres.
26076634	2	45	theme	cross-linking	276:288	arg1	microsphere					299:309	the pure cross-linking chitosan microsphere	267:309	the pure cross-linking chitosan microsphere	267:309	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	4	46	theme	Ni	667:668	arg1	adsorption					634:643	the adsorption	630:643	the adsorption of Cd(II), Cu(II) and Ni(II)	630:672	In single system, the equilibrium studies showed that the adsorption of Cd(II), Cu(II) and Ni(II) followed the Langmuir model and the pseudo-second-order kinetic model.
26076634	2	47	contain	have	364:367	arg2	capacity					385:392	better sorption capacity	369:392	better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1)	369:448	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	2	47	contain	have	364:367	arg1	microsphere					338:348	the nano-hybrid composite microsphere	312:348	the nano-hybrid composite microsphere	312:348	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	3	48	theme	Cd	497:498	arg1	microsphere					482:492	the microsphere	478:492	the microsphere of Cd(II), Cu(II) and Ni(II)	478:521	The adsorption behavior of the microsphere of Cd(II), Cu(II) and Ni(II) was investigated in single and binary metal systems.
26076634	2	49	theme	pure	271:274	arg1	microsphere					299:309	the pure cross-linking chitosan microsphere	267:309	the pure cross-linking chitosan microsphere	267:309	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	6	50	located	found	884:888	arg1	system					838:843	binary system	831:843	binary system	831:843	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	6	50	located	found	884:888	arg2	antagonistic					896:907	antagonistic	896:907	antagonistic	896:907	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	6	50	located	found	884:888	arg2	action					859:864	the combined action	846:864	the combined action of the metals	846:878	In binary system, the combined action of the metals was found to be antagonistic and the metal sorption followed the order of Cu(II)>Cd(II)>Ni(II).
26076634	4	51	theme	pseudo-second-order	710:728	arg1	model					738:742	the pseudo-second-order kinetic model	706:742	the pseudo-second-order kinetic model	706:742	In single system, the equilibrium studies showed that the adsorption of Cd(II), Cu(II) and Ni(II) followed the Langmuir model and the pseudo-second-order kinetic model.
26076634	5	52	theme	adsorption	792:801	arg1	spontaneous					815:825	spontaneous	815:825	spontaneous	815:825	The negative values of (ΔG) suggested that the adsorption process was spontaneous.
26076634	5	52	theme	adsorption	792:801	arg1	process					803:809	the adsorption process	788:809	the adsorption process	788:809	The negative values of (ΔG) suggested that the adsorption process was spontaneous.
26076634	3	53	theme	microsphere	482:492	arg1	behavior					466:473	The adsorption behavior	451:473	The adsorption behavior of the microsphere of Cd(II), Cu(II) and Ni(II)	451:521	The adsorption behavior of the microsphere of Cd(II), Cu(II) and Ni(II) was investigated in single and binary metal systems.
26076634	1	54	theme	nano-hybrid	145:155	arg1	microsphere					167:177	Chitosan/rectorie (CTS/REC) nano-hybrid composite microsphere	117:177	Chitosan/rectorie (CTS/REC) nano-hybrid composite microsphere	117:177	Chitosan/rectorie (CTS/REC) nano-hybrid composite microsphere was prepared by changing the proportion of CTS/REC with 2:1, 3:1 and 4:1.
26076634	2	55	theme	nano-hybrid	316:326	arg1	microsphere					338:348	the nano-hybrid composite microsphere	312:348	the nano-hybrid composite microsphere	312:348	Compared with the pure cross-linking chitosan microsphere, the nano-hybrid composite microsphere was proved to have better sorption capacity of Cd(II), Cu(II) and Ni(II), especially 2:1(CTS/REC-1).
26076634	8	56	theme	FT-IR	1109:1113	arg1	spectra					1123:1129	The FT-IR and XPS spectra	1105:1129	spectra	1123:1129	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
26076634	4	57	theme	equilibrium	598:608	arg1	studies					610:616	the equilibrium studies	594:616	the equilibrium studies	594:616	In single system, the equilibrium studies showed that the adsorption of Cd(II), Cu(II) and Ni(II) followed the Langmuir model and the pseudo-second-order kinetic model.
26076634	8	58	theme	XPS	1119:1121	arg1	spectra					1123:1129	The FT-IR and XPS spectra	1105:1129	spectra	1123:1129	The FT-IR and XPS spectra showed that coordination bonds were formed between Cd(II), Cu(II) and Ni(II) and the nitrogen atoms of cross-linking CTS/REC nano-hybrid composite microspheres.
29031849	0	0	theme	ethanol-water	78:90	arg1	solutions					92:100	ethanol-water solutions	78:100	ethanol-water solutions	78:100	Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions.
29031849	4	1	with	bioactivities	701:713	arg1	additives					750:758	these additives	744:758	these additives	744:758	The results showed that the bioactivities of spray dried lysozyme with these additives were about 5-10% higher than that without additives.
29031849	1	2	theme	spray	251:255	arg1	process					264:270	the spray drying process	247:270	the spray drying process	247:270	Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process.
29031849	5	3	theme	solutions	1007:1015	arg1	time					990:993	the storage time	978:993	the storage time of the feed solutions prior to spray drying	978:1037	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	3	4	from	temperature	533:543	arg1	solution					586:593	the feed solution	577:593	the feed solution	577:593	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	1	5	theme	drying	257:262	arg1	process					264:270	the spray drying process	247:270	the spray drying process	247:270	Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process.
29031849	7	6	theme	spray	1427:1431	arg1	drying					1433:1438	spray drying	1427:1438	spray drying from a water-ethanol mixture	1427:1467	This suggests that the bioactivity of lysozyme could be preserved adequately by optimization of both the formulation composition and process conditions even when spray drying from a water-ethanol mixture.
29031849	6	7	theme	powder	1217:1222	arg1	reconstitution					1183:1196	reconstitution	1183:1196	reconstitution of the spray dried powder in water	1183:1231	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	6	8	theme	lysozyme	1147:1154	arg1	structures					1133:1142	the native structures	1122:1142	the native structures of lysozyme	1122:1154	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	4	9	theme	lysozyme	730:737	arg1	higher					777:782	higher	777:782	higher	777:782	The results showed that the bioactivities of spray dried lysozyme with these additives were about 5-10% higher than that without additives.
29031849	4	9	theme	lysozyme	730:737	arg1	bioactivities					701:713	the bioactivities	697:713	the bioactivities of spray dried lysozyme with these additives	697:758	The results showed that the bioactivities of spray dried lysozyme with these additives were about 5-10% higher than that without additives.
29031849	2	10	from	mixtures	402:409	arg1	drying					376:381	spray drying	370:381	spray drying from water-ethanol mixtures	370:409	In this study, lysozyme was chosen as a model pharmaceutical protein to study these aspects when spray drying from water-ethanol mixtures.
29031849	7	11	theme	conditions	1406:1415	arg1	optimization					1345:1356	optimization	1345:1356	optimization of both the formulation composition and process conditions	1345:1415	This suggests that the bioactivity of lysozyme could be preserved adequately by optimization of both the formulation composition and process conditions even when spray drying from a water-ethanol mixture.
29031849	6	12	theme	dried	1211:1215	arg1	powder					1217:1222	the spray dried powder	1201:1222	the spray dried powder	1201:1222	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	5	13	theme	similar	945:951	arg1	findings					953:960	similar findings	945:960	similar findings	945:960	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	6	14	theme	Circular	1078:1085	arg1	CD					1098:1099	CD	1098:1099	CD	1098:1099	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	6	14	theme	Circular	1078:1085	arg1	Dichroism					1087:1095	Circular Dichroism	1078:1095	Circular Dichroism (CD) results	1078:1108	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	6	15	theme	spray	1205:1209	arg1	powder					1217:1222	the spray dried powder	1201:1222	the spray dried powder	1201:1222	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	5	16	theme	lysozyme	848:855	arg1	bioactivity					817:827	The bioactivity	813:827	The bioactivity of the spray dried lysozyme	813:855	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	3	17	theme	formulation	426:436	arg1	saline					492:497	phosphate-buffered saline	473:497	phosphate-buffered saline	473:497	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	17	theme	formulation	426:436	arg1	trehalose					449:457	trehalose	449:457	trehalose	449:457	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	17	theme	formulation	426:436	arg1	additives					438:446	formulation additives	426:446	formulation additives (trehalose, Tween 20 and phosphate-buffered saline)	426:498	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	17	theme	formulation	426:436	arg1	Tween					460:464	Tween	460:464	Tween	460:464	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	18	theme	inlet	527:531	arg1	temperature					533:543	inlet temperature	527:543	inlet temperature	527:543	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	18	theme	inlet	527:531	arg1	conditions					515:524	processing conditions	504:524	processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process)	504:626	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	1	19	theme	structural	184:193	arg1	integrity					195:203	the structural integrity	180:203	the structural integrity as well as the bioactivity of proteins in the spray drying process	180:270	Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process.
29031849	7	20	theme	process	1398:1404	arg1	conditions					1406:1415	process conditions	1398:1415	process conditions	1398:1415	This suggests that the bioactivity of lysozyme could be preserved adequately by optimization of both the formulation composition and process conditions even when spray drying from a water-ethanol mixture.
29031849	7	21	from	mixture	1461:1467	arg1	drying					1433:1438	spray drying	1427:1438	spray drying from a water-ethanol mixture	1427:1467	This suggests that the bioactivity of lysozyme could be preserved adequately by optimization of both the formulation composition and process conditions even when spray drying from a water-ethanol mixture.
29031849	4	22	theme	dried	724:728	arg1	lysozyme					730:737	spray dried lysozyme	718:737	spray dried lysozyme	718:737	The results showed that the bioactivities of spray dried lysozyme with these additives were about 5-10% higher than that without additives.
29031849	2	23	theme	spray	370:374	arg1	drying					376:381	spray drying	370:381	spray drying from water-ethanol mixtures	370:409	In this study, lysozyme was chosen as a model pharmaceutical protein to study these aspects when spray drying from water-ethanol mixtures.
29031849	7	24	theme	formulation	1370:1380	arg1	composition					1382:1392	the formulation composition	1366:1392	the formulation composition	1366:1392	This suggests that the bioactivity of lysozyme could be preserved adequately by optimization of both the formulation composition and process conditions even when spray drying from a water-ethanol mixture.
29031849	7	25	theme	water-ethanol	1447:1459	arg1	mixture					1461:1467	a water-ethanol mixture	1445:1467	a water-ethanol mixture	1445:1467	This suggests that the bioactivity of lysozyme could be preserved adequately by optimization of both the formulation composition and process conditions even when spray drying from a water-ethanol mixture.
29031849	3	26	theme	lysozyme	565:572	arg1	temperature					533:543	inlet temperature	527:543	inlet temperature	527:543	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	26	theme	lysozyme	565:572	arg1	time					557:560	storage time	549:560	storage time	549:560	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	26	theme	lysozyme	565:572	arg1	conditions					515:524	processing conditions	504:524	processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process)	504:626	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	27	from	time	557:560	arg1	solution					586:593	the feed solution	577:593	the feed solution	577:593	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	4	28	theme	spray	718:722	arg1	lysozyme					730:737	spray dried lysozyme	718:737	spray dried lysozyme	718:737	The results showed that the bioactivities of spray dried lysozyme with these additives were about 5-10% higher than that without additives.
29031849	5	29	from	decrease	886:893	arg1	80°C					934:937	80°C	934:937	80°C	934:937	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	5	29	from	decrease	886:893	arg1	temperature					908:918	the inlet temperature	898:918	the inlet temperature from 130°C to 80°C	898:937	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	1	30	theme	Formulation	103:113	arg1	composition					115:125	Formulation composition	103:125	Formulation composition	103:125	Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process.
29031849	0	31	theme	factors	17:23	arg1	Investigation					0:12	Investigation	0:12	Investigation of factors affecting the stability of lysozyme spray	0:65	Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions.
29031849	3	32	dep	conditions	515:524	arg1	temperature					533:543	inlet temperature	527:543	inlet temperature	527:543	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	32	dep	conditions	515:524	arg1	time					557:560	storage time	549:560	storage time	549:560	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	32	dep	conditions	515:524	arg1	conditions					515:524	processing conditions	504:524	processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process)	504:626	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	5	33	theme	dried	842:846	arg1	lysozyme					848:855	the spray dried lysozyme	832:855	the spray dried lysozyme	832:855	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	6	34	dep	Infrared	1058:1065	arg1	FTIR					1068:1071	FTIR	1068:1071	FTIR	1068:1071	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	2	35	theme	pharmaceutical	319:332	arg1	protein					334:340	a model pharmaceutical protein	311:340	a model pharmaceutical protein	311:340	In this study, lysozyme was chosen as a model pharmaceutical protein to study these aspects when spray drying from water-ethanol mixtures.
29031849	2	35	theme	pharmaceutical	319:332	arg1	lysozyme					288:295	lysozyme	288:295	lysozyme	288:295	In this study, lysozyme was chosen as a model pharmaceutical protein to study these aspects when spray drying from water-ethanol mixtures.
29031849	5	36	theme	spray	1026:1030	arg1	drying					1032:1037	spray drying	1026:1037	spray drying	1026:1037	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	3	37	theme	feed	581:584	arg1	solution					586:593	the feed solution	577:593	the feed solution	577:593	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	7	38	theme	composition	1382:1392	arg1	optimization					1345:1356	optimization	1345:1356	optimization of both the formulation composition and process conditions	1345:1415	This suggests that the bioactivity of lysozyme could be preserved adequately by optimization of both the formulation composition and process conditions even when spray drying from a water-ethanol mixture.
29031849	5	39	theme	spray	836:840	arg1	lysozyme					848:855	the spray dried lysozyme	832:855	the spray dried lysozyme	832:855	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	6	40	from	reconstitution	1183:1196	arg1	water					1227:1231	water	1227:1231	water	1227:1231	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	5	41	theme	storage	982:988	arg1	time					990:993	the storage time	978:993	the storage time of the feed solutions prior to spray drying	978:1037	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	2	42	theme	water-ethanol	388:400	arg1	mixtures					402:409	water-ethanol mixtures	388:409	water-ethanol mixtures	388:409	In this study, lysozyme was chosen as a model pharmaceutical protein to study these aspects when spray drying from water-ethanol mixtures.
29031849	0	43	theme	lysozyme	52:59	arg1	spray					61:65	lysozyme spray	52:65	lysozyme spray	52:65	Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions.
29031849	6	44	theme	drying	1249:1254	arg1	process					1256:1262	the spray drying process	1239:1262	the spray drying process	1239:1262	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	3	45	from	effect	416:421	arg1	bioactivity					643:653	the protein bioactivity	631:653	the protein bioactivity	631:653	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	7	46	theme	lysozyme	1303:1310	arg1	bioactivity					1288:1298	the bioactivity	1284:1298	the bioactivity of lysozyme	1284:1310	This suggests that the bioactivity of lysozyme could be preserved adequately by optimization of both the formulation composition and process conditions even when spray drying from a water-ethanol mixture.
29031849	1	47	from	bioactivity	220:230	arg1	process					264:270	the spray drying process	247:270	the spray drying process	247:270	Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process.
29031849	3	48	theme	phosphate-buffered	473:490	arg1	additives					438:446	formulation additives	426:446	formulation additives (trehalose, Tween 20 and phosphate-buffered saline)	426:498	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	48	theme	phosphate-buffered	473:490	arg1	saline					492:497	phosphate-buffered saline	473:497	phosphate-buffered saline	473:497	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	6	49	theme	spray	1243:1247	arg1	process					1256:1262	the spray drying process	1239:1262	the spray drying process	1239:1262	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	3	50	dep	additives	438:446	arg1	additives					438:446	formulation additives	426:446	formulation additives (trehalose, Tween 20 and phosphate-buffered saline)	426:498	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	50	dep	additives	438:446	arg1	trehalose					449:457	trehalose	449:457	trehalose	449:457	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	50	dep	additives	438:446	arg1	saline					492:497	phosphate-buffered saline	473:497	phosphate-buffered saline	473:497	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	50	dep	additives	438:446	arg1	Tween					460:464	Tween	460:464	Tween	460:464	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	1	51	theme	processing	131:140	arg1	conditions					142:151	processing conditions	131:151	processing conditions	131:151	Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process.
29031849	5	52	theme	inlet	902:906	arg1	temperature					908:918	the inlet temperature	898:918	the inlet temperature from 130°C to 80°C	898:937	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	5	53	theme	feed	1002:1005	arg1	solutions					1007:1015	the feed solutions	998:1015	the feed solutions	998:1015	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	3	54	theme	spray	606:610	arg1	process					619:625	the spray drying process	602:625	the spray drying process	602:625	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	2	55	theme	model	313:317	arg1	protein					334:340	a model pharmaceutical protein	311:340	a model pharmaceutical protein	311:340	In this study, lysozyme was chosen as a model pharmaceutical protein to study these aspects when spray drying from water-ethanol mixtures.
29031849	2	55	theme	model	313:317	arg1	lysozyme					288:295	lysozyme	288:295	lysozyme	288:295	In this study, lysozyme was chosen as a model pharmaceutical protein to study these aspects when spray drying from water-ethanol mixtures.
29031849	3	56	theme	storage	549:555	arg1	time					557:560	storage time	549:560	storage time	549:560	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	56	theme	storage	549:555	arg1	conditions					515:524	processing conditions	504:524	processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process)	504:626	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	57	theme	protein	635:641	arg1	bioactivity					643:653	the protein bioactivity	631:653	the protein bioactivity	631:653	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	58	theme	drying	612:617	arg1	process					619:625	the spray drying process	602:625	the spray drying process	602:625	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	1	59	from	integrity	195:203	arg1	process					264:270	the spray drying process	247:270	the spray drying process	247:270	Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process.
29031849	5	60	dep	80°C	934:937	arg1	to					931:932	to	931:932	to	931:932	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	1	61	theme	proteins	235:242	arg1	integrity					195:203	the structural integrity	180:203	the structural integrity as well as the bioactivity of proteins in the spray drying process	180:270	Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process.
29031849	1	61	theme	proteins	235:242	arg1	bioactivity					220:230	the bioactivity	216:230	the structural integrity as well as the bioactivity of proteins in the spray drying process	180:270	Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process.
29031849	5	62	from	80°C	934:937	arg1	decrease					886:893	a decrease	884:893	a decrease in the inlet temperature from 130°C to 80°C	884:937	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	5	62	from	80°C	934:937	arg1	temperature					908:918	the inlet temperature	898:918	the inlet temperature from 130°C to 80°C	898:937	The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying.
29031849	3	63	theme	processing	504:513	arg1	temperature					533:543	inlet temperature	527:543	inlet temperature	527:543	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	63	theme	processing	504:513	arg1	time					557:560	storage time	549:560	storage time	549:560	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	63	theme	processing	504:513	arg1	conditions					515:524	processing conditions	504:524	processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process)	504:626	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	3	64	theme	additives	438:446	arg1	effect					416:421	The effect	412:421	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity	412:653	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
29031849	0	65	theme	spray	61:65	arg1	stability					39:47	the stability	35:47	the stability of lysozyme spray	35:65	Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions.
29031849	6	66	theme	Dichroism	1087:1095	arg1	results					1102:1108	Circular Dichroism (CD) results	1078:1108	Circular Dichroism (CD) results	1078:1108	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	6	67	theme	native	1126:1131	arg1	structures					1133:1142	the native structures	1122:1142	the native structures of lysozyme	1122:1154	Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process.
29031849	3	68	theme	conditions	515:524	arg1	effect					416:421	The effect	412:421	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity	412:653	The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated.
25238818	7	0	theme	faecal	1241:1246	arg1	samples					1248:1254	faecal samples	1241:1254	faecal samples	1241:1254	was increased in XOS-fed rats as compared to CON in faecal samples after the intervention.
25238818	3	1	from	integrity	528:536	arg1	rats					553:556	male Wistar rats	541:556	male Wistar rats	541:556	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	12	2	theme	gene	1973:1976	arg1	expression					1927:1936	expression	1927:1936	expression of a single tight junction protein gene	1927:1976	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	10	3	theme	intestinal	1561:1570	arg1	integrity					1572:1580	intestinal integrity	1561:1580	intestinal integrity	1561:1580	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	11	4	theme	occludin	1646:1653	arg1	expression					1632:1641	the expression	1628:1641	the expression	1628:1641	However, the expression of occludin, which is part of the tight junction complex, was increased in the XOS group compared to the CON group.
25238818	11	4	theme	occludin	1646:1653	arg1	part					1665:1668	part	1665:1668	part of the tight junction complex	1665:1698	However, the expression of occludin, which is part of the tight junction complex, was increased in the XOS group compared to the CON group.
25238818	5	5	theme	gene	888:891	arg1	sequencing					893:902	16S rRNA gene sequencing	879:902	16S rRNA gene sequencing	879:902	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	12	6	theme	single	1943:1948	arg1	protein					1965:1971	a single tight junction protein	1941:1971	a single tight junction protein gene	1941:1976	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	6	7	theme	Bifidobacterium	1168:1182	arg1	abundance					1155:1163	the relative abundance	1142:1163	the relative abundance of Bifidobacterium	1142:1182	However as hypothesised, the relative abundance of Bifidobacterium spp.
25238818	4	8	theme	supplementation	689:703	arg1	effects					678:684	effects	678:684	effects of supplementation	678:703	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	2	9	theme	intestinal	340:349	arg1	integrity					351:359	intestinal integrity	340:359	intestinal integrity	340:359	Previous studies indicate that also changes in intestinal integrity may occur.
25238818	12	10	with	Supplementation	1771:1785	arg1	pseudolongum					1827:1838	a commensal Bifidobacterium pseudolongum	1799:1838	a commensal Bifidobacterium pseudolongum	1799:1838	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	12	10	with	Supplementation	1771:1785	arg1	XOS					1792:1794	XOS	1792:1794	XOS	1792:1794	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	12	11	theme	junction	1956:1963	arg1	protein					1965:1971	a single tight junction protein	1941:1971	a single tight junction protein gene	1941:1976	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	11	12	theme	CON	1748:1750	arg1	group					1752:1756	the CON group	1744:1756	the CON group	1744:1756	However, the expression of occludin, which is part of the tight junction complex, was increased in the XOS group compared to the CON group.
25238818	1	13	theme	bacterial	232:240	arg1	composition					242:252	the bacterial composition	228:252	the bacterial composition	228:252	BACKGROUND Consumption of prebiotics may modulate gut microbiota, subsequently affecting the bacterial composition, metabolite profile, and human health.
25238818	0	14	from	effect	99:104	arg1	integrity					120:128	intestinal integrity	109:128	intestinal integrity in rats	109:136	Dietary xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats.
25238818	4	15	attach	isolated	757:764	arg2	BIF					752:754	BIF	752:754	BIF	752:754	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	4	15	attach	isolated	757:764	arg2	pseudolongum					738:749	a commensal Bifidobacterium pseudolongum	710:749	a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats	710:803	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	4	15	attach	isolated	757:764	arg1	breed					780:784	the same breed	771:784	the same breed of laboratory rats	771:803	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	5	16	theme	caecal	836:841	arg1	composition					853:863	faecal and caecal bacterial composition	825:863	faecal and caecal bacterial composition determined by 16S rRNA gene sequencing	825:902	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	5	17	theme	16S	879:881	arg1	sequencing					893:902	16S rRNA gene sequencing	879:902	16S rRNA gene sequencing	879:902	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	10	18	theme	BIF	1607:1609	arg1	groups					1611:1616	the BIF groups	1603:1616	the BIF groups	1603:1616	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	1	19	theme	BACKGROUND	139:148	arg1	Consumption					150:160	BACKGROUND Consumption	139:160	BACKGROUND Consumption of prebiotics	139:174	BACKGROUND Consumption of prebiotics may modulate gut microbiota, subsequently affecting the bacterial composition, metabolite profile, and human health.
25238818	5	20	theme	BIF	1063:1065	arg1	groups					1067:1072	BIF groups	1063:1072	BIF groups	1063:1072	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	5	21	from	Changes	814:820	arg1	composition					853:863	faecal and caecal bacterial composition	825:863	faecal and caecal bacterial composition determined by 16S rRNA gene sequencing	825:902	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	4	22	theme	Bifidobacterium	722:736	arg1	BIF					752:754	BIF	752:754	BIF	752:754	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	4	22	theme	Bifidobacterium	722:736	arg1	pseudolongum					738:749	a commensal Bifidobacterium pseudolongum	710:749	a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats	710:803	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	0	23	from	integrity	120:128	arg1	rats					133:136	rats	133:136	rats	133:136	Dietary xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats.
25238818	1	24	theme	gut	189:191	arg1	microbiota					193:202	gut microbiota	189:202	gut microbiota	189:202	BACKGROUND Consumption of prebiotics may modulate gut microbiota, subsequently affecting the bacterial composition, metabolite profile, and human health.
25238818	0	25	theme	intestinal	109:118	arg1	integrity					120:128	intestinal integrity	109:128	intestinal integrity in rats	109:136	Dietary xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats.
25238818	5	26	theme	multiple	1095:1102	arg1	comparisons					1104:1114	multiple comparisons	1095:1114	multiple comparisons	1095:1114	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	3	27	dep	microbiota	502:511	arg1	the					494:496	the	494:496	the	494:496	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	10	28	theme	caecum	1485:1490	arg1	water					1492:1496	extracted caecum water	1475:1496	extracted caecum water	1475:1496	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	4	29	theme	rats	800:803	arg1	breed					780:784	the same breed	771:784	the same breed of laboratory rats	771:803	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	3	30	theme	xylo-oligosaccharides	463:483	arg1	effect					430:435	the effect	426:435	the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats	426:556	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	7	31	theme	XOS-fed	1206:1212	arg1	rats					1214:1217	XOS-fed rats	1206:1217	XOS-fed rats	1206:1217	was increased in XOS-fed rats as compared to CON in faecal samples after the intervention.
25238818	5	32	theme	selected	929:936	arg1	groups					948:953	selected bacterial groups	929:953	selected bacterial groups	929:953	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	3	33	theme	putative	444:451	arg1	xylo-oligosaccharides					463:483	the putative prebiotic xylo-oligosaccharides	440:483	the putative prebiotic xylo-oligosaccharides (XOS)	440:489	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	3	33	theme	putative	444:451	arg1	XOS					486:488	XOS	486:488	XOS	486:488	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	0	34	theme	Dietary	0:6	arg1	xylo-oligosaccharide					8:27	Dietary xylo-oligosaccharide	0:27	Dietary xylo-oligosaccharide	0:27	Dietary xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats.
25238818	5	35	theme	overall	973:979	arg1	composition					991:1001	the overall bacterial composition	969:1001	the overall bacterial composition	969:1001	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	10	36	theme	resistance	1518:1527	arg1	assay					1535:1539	trans-epithelial resistance (TER) assay	1501:1539	trans-epithelial resistance (TER) assay	1501:1539	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	4	37	theme	same	775:778	arg1	breed					780:784	the same breed	771:784	the same breed of laboratory rats	771:803	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	5	38	theme	bacterial	843:851	arg1	composition					853:863	faecal and caecal bacterial composition	825:863	faecal and caecal bacterial composition determined by 16S rRNA gene sequencing	825:902	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	1	39	theme	prebiotics	165:174	arg1	Consumption					150:160	BACKGROUND Consumption	139:160	BACKGROUND Consumption of prebiotics	139:174	BACKGROUND Consumption of prebiotics may modulate gut microbiota, subsequently affecting the bacterial composition, metabolite profile, and human health.
25238818	11	40	theme	tight	1677:1681	arg1	complex					1692:1698	the tight junction complex	1673:1698	the tight junction complex	1673:1698	However, the expression of occludin, which is part of the tight junction complex, was increased in the XOS group compared to the CON group.
25238818	9	41	theme	caecum	1352:1357	arg1	content					1359:1365	caecum content	1352:1365	caecum content	1352:1365	was increased in both the XOS and BIF groups in caecum content compared to CON.
25238818	12	42	theme	limited	1849:1855	arg1	effects					1857:1863	very limited effects	1844:1863	very limited effects on intestinal integrity in rats	1844:1895	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	3	43	theme	gut	498:500	arg1	microbiota					502:511	gut microbiota	498:511	gut microbiota	498:511	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	1	44	theme	metabolite	255:264	arg1	profile					266:272	metabolite profile	255:272	metabolite profile	255:272	BACKGROUND Consumption of prebiotics may modulate gut microbiota, subsequently affecting the bacterial composition, metabolite profile, and human health.
25238818	12	45	theme	Bifidobacterium	1811:1825	arg1	pseudolongum					1827:1838	a commensal Bifidobacterium pseudolongum	1799:1838	a commensal Bifidobacterium pseudolongum	1799:1838	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	4	46	theme	expected	616:623	arg1	effect					637:642	the expected bifidogenic effect	612:642	the expected bifidogenic effect of XOS	612:649	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	11	47	theme	complex	1692:1698	arg1	expression					1632:1641	the expression	1628:1641	the expression	1628:1641	However, the expression of occludin, which is part of the tight junction complex, was increased in the XOS group compared to the CON group.
25238818	11	47	theme	complex	1692:1698	arg1	part					1665:1668	part	1665:1668	part of the tight junction complex	1665:1698	However, the expression of occludin, which is part of the tight junction complex, was increased in the XOS group compared to the CON group.
25238818	3	48	theme	male	541:544	arg1	rats					553:556	male Wistar rats	541:556	male Wistar rats	541:556	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	12	49	theme	CONCLUSIONS	1759:1769	arg1	Supplementation					1771:1785	CONCLUSIONS Supplementation	1759:1785	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum	1759:1838	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	12	50	contain	had	1840:1842	arg2	effects					1857:1863	very limited effects	1844:1863	very limited effects on intestinal integrity in rats	1844:1895	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	12	50	contain	had	1840:1842	arg1	Supplementation					1771:1785	CONCLUSIONS Supplementation	1759:1785	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum	1759:1838	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	1	51	theme	human	279:283	arg1	health					285:290	human health	279:290	human health	279:290	BACKGROUND Consumption of prebiotics may modulate gut microbiota, subsequently affecting the bacterial composition, metabolite profile, and human health.
25238818	12	52	theme	XOS	1996:1998	arg1	group					2000:2004	the XOS group	1992:2004	the XOS group	1992:2004	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	10	53	theme	Intestinal	1384:1393	arg1	permeability					1395:1406	Intestinal permeability	1384:1406	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay	1384:1539	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	12	54	theme	protein	1965:1971	arg1	gene					1973:1976	a single tight junction protein gene	1941:1976	a single tight junction protein gene	1941:1976	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	10	55	dep	XOS	1596:1598	arg1	the					1592:1594	the	1592:1594	the	1592:1594	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	5	56	theme	rRNA	883:886	arg1	sequencing					893:902	16S rRNA gene sequencing	879:902	16S rRNA gene sequencing	879:902	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	3	57	from	microbiota	502:511	arg1	rats					553:556	male Wistar rats	541:556	male Wistar rats	541:556	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	12	58	from	effects	1857:1863	arg1	integrity					1879:1887	intestinal integrity	1868:1887	intestinal integrity	1868:1887	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	12	58	from	effects	1857:1863	arg1	rats					1892:1895	rats	1892:1895	rats	1892:1895	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	5	59	dep	RESULTS	806:812	arg1	Changes					814:820	Changes	814:820	Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing	814:902	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	12	60	theme	tight	1950:1954	arg1	protein					1965:1971	a single tight junction protein	1941:1971	a single tight junction protein gene	1941:1976	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	6	61	theme	relative	1146:1153	arg1	abundance					1155:1163	the relative abundance	1142:1163	the relative abundance of Bifidobacterium	1142:1182	However as hypothesised, the relative abundance of Bifidobacterium spp.
25238818	4	62	theme	XOS	647:649	arg1	effect					637:642	the expected bifidogenic effect	612:642	the expected bifidogenic effect of XOS	612:649	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	5	63	theme	bacterial	938:946	arg1	groups					948:953	selected bacterial groups	929:953	selected bacterial groups	929:953	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	2	64	theme	Previous	293:300	arg1	studies					302:308	Previous studies	293:308	Previous studies	293:308	Previous studies indicate that also changes in intestinal integrity may occur.
25238818	7	65	from	CON	1234:1236	arg1	samples					1248:1254	faecal samples	1241:1254	faecal samples	1241:1254	was increased in XOS-fed rats as compared to CON in faecal samples after the intervention.
25238818	11	66	theme	XOS	1722:1724	arg1	group					1726:1730	the XOS group	1718:1730	the XOS group	1718:1730	However, the expression of occludin, which is part of the tight junction complex, was increased in the XOS group compared to the CON group.
25238818	0	67	theme	limited	91:97	arg1	effect					99:104	limited effect	91:104	limited effect on intestinal integrity in rats	91:136	Dietary xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats.
25238818	5	68	theme	quantitative	908:919	arg1	PCR					921:923	quantitative PCR	908:923	quantitative PCR for selected bacterial groups	908:953	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	3	69	from	effect	430:435	arg1	microbiota					502:511	gut microbiota	498:511	gut microbiota	498:511	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	3	69	from	effect	430:435	arg1	integrity					528:536	intestinal integrity	517:536	intestinal integrity	517:536	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	2	70	from	changes	329:335	arg1	integrity					351:359	intestinal integrity	340:359	intestinal integrity	340:359	Previous studies indicate that also changes in intestinal integrity may occur.
25238818	4	71	theme	commensal	712:720	arg1	BIF					752:754	BIF	752:754	BIF	752:754	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	4	71	theme	commensal	712:720	arg1	pseudolongum					738:749	a commensal Bifidobacterium pseudolongum	710:749	a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats	710:803	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	12	72	theme	significant	1905:1915	arg1	change					1917:1922	only significant change	1900:1922	only significant change in expression of a single tight junction protein gene	1900:1976	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	10	73	theme	FITC-dextran	1430:1441	arg1	permeability					1443:1454	FITC-dextran permeability	1430:1454	FITC-dextran permeability	1430:1454	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	9	74	from	groups	1342:1347	arg1	content					1359:1365	caecum content	1352:1365	caecum content	1352:1365	was increased in both the XOS and BIF groups in caecum content compared to CON.
25238818	10	75	theme	extracted	1475:1483	arg1	water					1492:1496	extracted caecum water	1475:1496	extracted caecum water	1475:1496	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	4	76	theme	laboratory	789:798	arg1	rats					800:803	laboratory rats	789:803	laboratory rats	789:803	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	5	77	theme	faecal	825:830	arg1	composition					853:863	faecal and caecal bacterial composition	825:863	faecal and caecal bacterial composition determined by 16S rRNA gene sequencing	825:902	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	9	78	theme	XOS	1330:1332	arg1	groups					1342:1347	both the XOS and BIF groups	1321:1347	groups	1342:1347	was increased in both the XOS and BIF groups in caecum content compared to CON.
25238818	4	79	theme	intestinal	573:582	arg1	integrity					584:592	intestinal integrity	573:592	intestinal integrity	573:592	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	12	80	theme	intestinal	1868:1877	arg1	integrity					1879:1887	intestinal integrity	1868:1887	intestinal integrity	1868:1887	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	3	81	theme	prebiotic	453:461	arg1	xylo-oligosaccharides					463:483	the putative prebiotic xylo-oligosaccharides	440:483	the putative prebiotic xylo-oligosaccharides (XOS)	440:489	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	3	81	theme	prebiotic	453:461	arg1	XOS					486:488	XOS	486:488	XOS	486:488	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	0	82	theme	intestinal	40:49	arg1	bifidobacteria					51:64	intestinal bifidobacteria	40:64	intestinal bifidobacteria	40:64	Dietary xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats.
25238818	9	83	theme	BIF	1338:1340	arg1	groups					1342:1347	both the XOS and BIF groups	1321:1347	groups	1342:1347	was increased in both the XOS and BIF groups in caecum content compared to CON.
25238818	5	84	theme	bacterial	981:989	arg1	composition					991:1001	the overall bacterial composition	969:1001	the overall bacterial composition	969:1001	RESULTS Changes in faecal and caecal bacterial composition determined by 16S rRNA gene sequencing and quantitative PCR for selected bacterial groups revealed that the overall bacterial composition did not differ markedly between the control (CON), XOS, and BIF groups, when correcting for multiple comparisons.
25238818	10	85	theme	trans-epithelial	1501:1516	arg1	TER					1530:1532	TER	1530:1532	TER	1530:1532	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	10	85	theme	trans-epithelial	1501:1516	arg1	resistance					1518:1527	trans-epithelial resistance	1501:1527	trans-epithelial resistance (TER) assay	1501:1539	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	4	86	from	changes	562:568	arg1	integrity					584:592	intestinal integrity	573:592	intestinal integrity	573:592	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	12	87	theme	commensal	1801:1809	arg1	pseudolongum					1827:1838	a commensal Bifidobacterium pseudolongum	1799:1838	a commensal Bifidobacterium pseudolongum	1799:1838	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	10	88	from	effect	1551:1556	arg1	groups					1611:1616	the BIF groups	1603:1616	the BIF groups	1603:1616	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	10	88	from	effect	1551:1556	arg1	XOS					1596:1598	XOS	1596:1598	XOS	1596:1598	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	10	88	from	effect	1551:1556	arg1	integrity					1572:1580	intestinal integrity	1561:1580	intestinal integrity	1561:1580	Intestinal permeability determined in vivo by FITC-dextran permeability and in vitro using extracted caecum water in trans-epithelial resistance (TER) assay showed no effect on intestinal integrity in either the XOS or the BIF groups.
25238818	3	89	theme	intestinal	517:526	arg1	integrity					528:536	intestinal integrity	517:536	intestinal integrity	517:536	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
25238818	4	90	theme	bifidogenic	625:635	arg1	effect					637:642	the expected bifidogenic effect	612:642	the expected bifidogenic effect of XOS	612:649	As changes in intestinal integrity may be related to the expected bifidogenic effect of XOS, we additionally addressed effects of supplementation with a commensal Bifidobacterium pseudolongum (BIF) isolated from the same breed of laboratory rats.
25238818	11	91	theme	junction	1683:1690	arg1	complex					1692:1698	the tight junction complex	1673:1698	the tight junction complex	1673:1698	However, the expression of occludin, which is part of the tight junction complex, was increased in the XOS group compared to the CON group.
25238818	12	92	from	change	1917:1922	arg1	expression					1927:1936	expression	1927:1936	expression of a single tight junction protein gene	1927:1976	CONCLUSIONS Supplementation with XOS or a commensal Bifidobacterium pseudolongum had very limited effects on intestinal integrity in rats as only significant change in expression of a single tight junction protein gene was found for the XOS group.
25238818	0	93	contain	has	87:89	arg1	xylo-oligosaccharide					8:27	Dietary xylo-oligosaccharide	0:27	Dietary xylo-oligosaccharide	0:27	Dietary xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats.
25238818	0	93	contain	has	87:89	arg2	effect					99:104	limited effect	91:104	limited effect on intestinal integrity in rats	91:136	Dietary xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats.
25238818	3	94	theme	Wistar	546:551	arg1	rats					553:556	male Wistar rats	541:556	male Wistar rats	541:556	In order to explore this further we have investigated the effect of the putative prebiotic xylo-oligosaccharides (XOS) on the gut microbiota and intestinal integrity in male Wistar rats.
24888552	0	0	theme	sensory	67:73	arg1	properties					75:84	the physicochemical and sensory properties	43:84	the physicochemical and sensory properties of frankfurter	43:99	Influence of bacterial cellulose (nata) on the physicochemical and sensory properties of frankfurter.
24888552	8	1	theme	product	1164:1170	arg1	quality					1172:1178	product quality	1164:1178	product quality comparable to the control	1164:1204	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	9	2	theme	ingredient	1340:1349	arg1	inclusion					1303:1311	the inclusion	1299:1311	the inclusion of a high-fiber functional ingredient, nata	1299:1355	PRACTICAL APPLICATION We demonstrated the manufacture of quality-improved frankfurters with the inclusion of a high-fiber functional ingredient, nata.
24888552	6	3	dep	%	689:689	arg1	to					684:685	to	684:685	to	684:685	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	1	4	theme	bacterial	226:234	arg1	cellulose					236:244	bacterial cellulose	226:244	bacterial cellulose (nata)	226:251	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	1	4	theme	bacterial	226:234	arg1	nata					247:250	nata	247:250	nata	247:250	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	9	5	theme	PRACTICAL	1207:1215	arg1	APPLICATION					1217:1227	PRACTICAL APPLICATION	1207:1227	PRACTICAL APPLICATION	1207:1227	PRACTICAL APPLICATION We demonstrated the manufacture of quality-improved frankfurters with the inclusion of a high-fiber functional ingredient, nata.
24888552	6	6	theme	textural	749:756	arg1	properties					788:797	the physicochemical, textural, sensory, and microbiological properties	728:797	the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters	728:825	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	1	7	theme	pork	164:167	arg1	frankfurters					169:180	pork frankfurters	164:180	pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata)	164:251	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	1	8	theme	cellulose	236:244	arg1	levels					203:208	different levels	193:208	different levels (10% to 30%) of bacterial cellulose (nata)	193:251	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	1	8	theme	cellulose	236:244	arg1	%					220:220	10% to 30%	211:220	10% to 30%	211:220	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	6	9	contain	have	700:703	arg1	Incorporation					641:653	Incorporation	641:653	Incorporation of nata at lower levels (10% to 20%)	641:690	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	6	9	contain	have	700:703	arg2	effects					717:723	detrimental effects	705:723	detrimental effects	705:723	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	0	10	from	Influence	0:8	arg1	properties					75:84	the physicochemical and sensory properties	43:84	the physicochemical and sensory properties of frankfurter	43:99	Influence of bacterial cellulose (nata) on the physicochemical and sensory properties of frankfurter.
24888552	7	11	theme	nata	864:867	arg1	level					849:853	a higher level	840:853	a higher level (30%) of nata	840:867	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	7	11	theme	nata	864:867	arg1	%					858:858	30%	856:858	30%	856:858	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	6	12	theme	physicochemical	732:746	arg1	properties					788:797	the physicochemical, textural, sensory, and microbiological properties	728:797	the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters	728:825	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	8	13	theme	high-fiber	1136:1145	arg1	nata					1147:1150	10% to 20% high-fiber nata	1125:1150	10% to 20% high-fiber nata	1125:1150	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	8	14	theme	frankfurter	1102:1112	arg1	manufacture					1075:1085	manufacture	1075:1085	manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata	1075:1150	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	3	15	theme	shear	461:465	arg1	values					467:472	shear values	461:472	shear values	461:472	For nata-added treatments, increasing levels of nata in frankfurters resulted in decreased textural hardness and shear values.
24888552	5	16	theme	juiciness	590:598	arg1	score					600:604	juiciness score	590:604	juiciness score	590:604	However, juiciness score did not differ for all treatments.
24888552	8	17	theme	comparable	1180:1189	arg1	quality					1172:1178	product quality	1164:1178	product quality comparable to the control	1164:1204	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	1	18	theme	frankfurters	169:180	arg1	characteristics					145:159	The physicochemical and sensory characteristics	113:159	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata)	102:251	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	0	19	theme	frankfurter	89:99	arg1	properties					75:84	the physicochemical and sensory properties	43:84	the physicochemical and sensory properties of frankfurter	43:99	Influence of bacterial cellulose (nata) on the physicochemical and sensory properties of frankfurter.
24888552	7	20	theme	hardness	1026:1033	arg1	values					1035:1040	higher sensory firmness and instrumental hardness values	985:1040	higher sensory firmness and instrumental hardness values	985:1040	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	6	21	theme	microbiological	772:786	arg1	properties					788:797	the physicochemical, textural, sensory, and microbiological properties	728:797	the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters	728:825	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	6	22	from	levels	672:677	arg1	Incorporation					641:653	Incorporation	641:653	Incorporation of nata at lower levels (10% to 20%)	641:690	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	6	23	theme	lower	666:670	arg1	levels					672:677	lower levels	666:677	lower levels (10% to 20%)	666:690	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	6	23	theme	lower	666:670	arg1	%					689:689	10% to 20%	680:689	10% to 20%	680:689	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	8	24	theme	10	1125:1126	arg1	%					1127:1127	%	1127:1127	%	1127:1127	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	6	25	theme	frankfurters	814:825	arg1	properties					788:797	the physicochemical, textural, sensory, and microbiological properties	728:797	the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters	728:825	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	7	26	theme	firmness	1000:1007	arg1	values					1035:1040	higher sensory firmness and instrumental hardness values	985:1040	higher sensory firmness and instrumental hardness values	985:1040	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	8	27	theme	composite	1056:1064	arg1	results					1066:1072	the composite results	1052:1072	the composite results	1052:1072	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	8	28	dep	%	1127:1127	arg1	to					1129:1130	to	1129:1130	to	1129:1130	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	1	29	theme	different	193:201	arg1	levels					203:208	different levels	193:208	different levels (10% to 30%) of bacterial cellulose (nata)	193:251	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	1	29	theme	different	193:201	arg1	%					220:220	10% to 30%	211:220	10% to 30%	211:220	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	3	30	theme	nata-added	352:361	arg1	treatments					363:372	nata-added treatments	352:372	nata-added treatments	352:372	For nata-added treatments, increasing levels of nata in frankfurters resulted in decreased textural hardness and shear values.
24888552	9	31	theme	quality-improved	1264:1279	arg1	frankfurters					1281:1292	quality-improved frankfurters	1264:1292	quality-improved frankfurters	1264:1292	PRACTICAL APPLICATION We demonstrated the manufacture of quality-improved frankfurters with the inclusion of a high-fiber functional ingredient, nata.
24888552	6	32	theme	detrimental	705:715	arg1	effects					717:723	detrimental effects	705:723	detrimental effects	705:723	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	7	33	theme	instrumental	1013:1024	arg1	hardness					1026:1033	instrumental hardness	1013:1033	instrumental hardness	1013:1033	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	6	34	theme	nata	658:661	arg1	Incorporation					641:653	Incorporation	641:653	Incorporation of nata at lower levels (10% to 20%)	641:690	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	2	35	theme	nata	342:345	arg1	addition					330:337	the addition	326:337	the addition of nata	326:345	Emulsion stability of the raw meat batter decreased with the addition of nata.
24888552	0	36	theme	cellulose	23:31	arg1	Influence					0:8	Influence	0:8	Influence of bacterial cellulose (nata) on the physicochemical and sensory properties of frankfurter.	0:100	Influence of bacterial cellulose (nata) on the physicochemical and sensory properties of frankfurter.
24888552	9	37	theme	frankfurters	1281:1292	arg1	manufacture					1249:1259	the manufacture	1245:1259	the manufacture of quality-improved frankfurters with the inclusion of a high-fiber functional ingredient, nata	1245:1355	PRACTICAL APPLICATION We demonstrated the manufacture of quality-improved frankfurters with the inclusion of a high-fiber functional ingredient, nata.
24888552	7	38	theme	higher	985:990	arg1	firmness					1000:1007	higher sensory firmness	985:1007	higher sensory firmness	985:1007	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	4	39	contain	had	518:520	arg2	scores					552:557	significantly higher firmness scores	522:557	significantly higher firmness scores	522:557	Sensory results indicated that N10 and N20 had significantly higher firmness scores than N30 and control.
24888552	4	39	contain	had	518:520	arg1	N20					514:516	N20	514:516	N20	514:516	Sensory results indicated that N10 and N20 had significantly higher firmness scores than N30 and control.
24888552	4	39	contain	had	518:520	arg1	N10					506:508	N10	506:508	N10	506:508	Sensory results indicated that N10 and N20 had significantly higher firmness scores than N30 and control.
24888552	3	40	theme	decreased	429:437	arg1	hardness					448:455	decreased textural hardness	429:455	decreased textural hardness	429:455	For nata-added treatments, increasing levels of nata in frankfurters resulted in decreased textural hardness and shear values.
24888552	0	41	theme	bacterial	13:21	arg1	nata					34:37	nata	34:37	nata	34:37	Influence of bacterial cellulose (nata) on the physicochemical and sensory properties of frankfurter.
24888552	0	41	theme	bacterial	13:21	arg1	cellulose					23:31	bacterial cellulose	13:31	bacterial cellulose (nata)	13:38	Influence of bacterial cellulose (nata) on the physicochemical and sensory properties of frankfurter.
24888552	4	42	theme	firmness	543:550	arg1	scores					552:557	significantly higher firmness scores	522:557	significantly higher firmness scores	522:557	Sensory results indicated that N10 and N20 had significantly higher firmness scores than N30 and control.
24888552	7	43	theme	sensory	992:998	arg1	firmness					1000:1007	higher sensory firmness	985:1007	higher sensory firmness	985:1007	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	6	44	theme	regular-fat	802:812	arg1	frankfurters					814:825	regular-fat frankfurters	802:825	regular-fat frankfurters	802:825	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	2	45	theme	Emulsion	269:276	arg1	stability					278:286	Emulsion stability	269:286	Emulsion stability of the raw meat batter	269:309	Emulsion stability of the raw meat batter decreased with the addition of nata.
24888552	3	46	theme	textural	439:446	arg1	hardness					448:455	decreased textural hardness	429:455	decreased textural hardness	429:455	For nata-added treatments, increasing levels of nata in frankfurters resulted in decreased textural hardness and shear values.
24888552	3	47	theme	increasing	375:384	arg1	levels					386:391	increasing levels	375:391	increasing levels of nata in frankfurters	375:415	For nata-added treatments, increasing levels of nata in frankfurters resulted in decreased textural hardness and shear values.
24888552	4	48	theme	higher	536:541	arg1	scores					552:557	significantly higher firmness scores	522:557	significantly higher firmness scores	522:557	Sensory results indicated that N10 and N20 had significantly higher firmness scores than N30 and control.
24888552	8	49	dep	%	1134:1134	arg1	%					1127:1127	%	1127:1127	%	1127:1127	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	3	50	theme	nata	396:399	arg1	levels					386:391	increasing levels	375:391	increasing levels of nata in frankfurters	375:415	For nata-added treatments, increasing levels of nata in frankfurters resulted in decreased textural hardness and shear values.
24888552	1	51	theme	%	213:213	arg1	levels					203:208	different levels	193:208	different levels (10% to 30%) of bacterial cellulose (nata)	193:251	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	1	51	theme	%	213:213	arg1	%					220:220	10% to 30%	211:220	10% to 30%	211:220	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	8	52	theme	20	1132:1133	arg1	%					1127:1127	%	1127:1127	%	1127:1127	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	1	53	contain	containing	182:191	arg1	frankfurters					169:180	pork frankfurters	164:180	pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata)	164:251	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	1	53	contain	containing	182:191	arg2	levels					203:208	different levels	193:208	different levels (10% to 30%) of bacterial cellulose (nata)	193:251	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	1	53	contain	containing	182:191	arg2	%					220:220	10% to 30%	211:220	10% to 30%	211:220	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	8	54	theme	regular-fat	1090:1100	arg1	frankfurter					1102:1112	regular-fat frankfurter	1090:1112	regular-fat frankfurter containing 10% to 20% high-fiber nata	1090:1150	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	7	55	from	frankfurters	964:975	arg1	levels					946:951	lower levels	940:951	lower levels of nata in frankfurters	940:975	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	7	56	theme	nata	956:959	arg1	levels					946:951	lower levels	940:951	lower levels of nata in frankfurters	940:975	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	1	57	dep	%	220:220	arg1	to					215:216	to	215:216	to	215:216	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	7	58	theme	lower	940:944	arg1	levels					946:951	lower levels	940:951	lower levels of nata in frankfurters	940:975	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	1	59	theme	UNLABELLED	102:111	arg1	characteristics					145:159	The physicochemical and sensory characteristics	113:159	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata)	102:251	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	3	60	from	levels	386:391	arg1	frankfurters					404:415	frankfurters	404:415	frankfurters	404:415	For nata-added treatments, increasing levels of nata in frankfurters resulted in decreased textural hardness and shear values.
24888552	9	61	dep	demonstrated	1232:1243	arg1	APPLICATION					1217:1227	PRACTICAL APPLICATION	1207:1227	PRACTICAL APPLICATION	1207:1227	PRACTICAL APPLICATION We demonstrated the manufacture of quality-improved frankfurters with the inclusion of a high-fiber functional ingredient, nata.
24888552	8	62	dep	nata	1147:1150	arg1	%					1134:1134	%	1134:1134	%	1134:1134	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	9	63	with	manufacture	1249:1259	arg1	inclusion					1303:1311	the inclusion	1299:1311	the inclusion of a high-fiber functional ingredient, nata	1299:1355	PRACTICAL APPLICATION We demonstrated the manufacture of quality-improved frankfurters with the inclusion of a high-fiber functional ingredient, nata.
24888552	2	64	theme	batter	304:309	arg1	stability					278:286	Emulsion stability	269:286	Emulsion stability of the raw meat batter	269:309	Emulsion stability of the raw meat batter decreased with the addition of nata.
24888552	7	65	theme	level	849:853	arg1	Addition					828:835	Addition	828:835	Addition of a higher level (30%) of nata	828:867	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	9	66	theme	high-fiber	1318:1327	arg1	nata					1352:1355	nata	1352:1355	nata	1352:1355	PRACTICAL APPLICATION We demonstrated the manufacture of quality-improved frankfurters with the inclusion of a high-fiber functional ingredient, nata.
24888552	9	66	theme	high-fiber	1318:1327	arg1	ingredient					1340:1349	a high-fiber functional ingredient	1316:1349	a high-fiber functional ingredient	1316:1349	PRACTICAL APPLICATION We demonstrated the manufacture of quality-improved frankfurters with the inclusion of a high-fiber functional ingredient, nata.
24888552	7	67	theme	comparable	890:899	arg1	properties					909:918	comparable sensory properties	890:918	comparable sensory properties	890:918	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	1	68	theme	physicochemical	117:131	arg1	characteristics					145:159	The physicochemical and sensory characteristics	113:159	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata)	102:251	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	7	69	theme	sensory	901:907	arg1	properties					909:918	comparable sensory properties	890:918	comparable sensory properties	890:918	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	7	70	from	levels	946:951	arg1	frankfurters					964:975	frankfurters	964:975	frankfurters	964:975	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	6	71	theme	sensory	759:765	arg1	properties					788:797	the physicochemical, textural, sensory, and microbiological properties	728:797	the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters	728:825	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	2	72	theme	raw	295:297	arg1	batter					304:309	the raw meat batter	291:309	the raw meat batter	291:309	Emulsion stability of the raw meat batter decreased with the addition of nata.
24888552	2	73	theme	meat	299:302	arg1	batter					304:309	the raw meat batter	291:309	the raw meat batter	291:309	Emulsion stability of the raw meat batter decreased with the addition of nata.
24888552	0	74	theme	physicochemical	47:61	arg1	properties					75:84	the physicochemical and sensory properties	43:84	the physicochemical and sensory properties of frankfurter	43:99	Influence of bacterial cellulose (nata) on the physicochemical and sensory properties of frankfurter.
24888552	8	75	contain	containing	1114:1123	arg2	nata					1147:1150	10% to 20% high-fiber nata	1125:1150	10% to 20% high-fiber nata	1125:1150	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	8	75	contain	containing	1114:1123	arg1	frankfurter					1102:1112	regular-fat frankfurter	1090:1112	regular-fat frankfurter containing 10% to 20% high-fiber nata	1090:1150	Based on the composite results, manufacture of regular-fat frankfurter containing 10% to 20% high-fiber nata resulted in product quality comparable to the control.
24888552	1	76	theme	sensory	137:143	arg1	characteristics					145:159	The physicochemical and sensory characteristics	113:159	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata)	102:251	UNLABELLED The physicochemical and sensory characteristics of pork frankfurters containing different levels (10% to 30%) of bacterial cellulose (nata) were evaluated.
24888552	7	77	theme	higher	842:847	arg1	level					849:853	a higher level	840:853	a higher level (30%) of nata	840:867	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	7	77	theme	higher	842:847	arg1	%					858:858	30%	856:858	30%	856:858	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	7	78	from	nata	956:959	arg1	frankfurters					964:975	frankfurters	964:975	frankfurters	964:975	Addition of a higher level (30%) of nata essentially produced comparable sensory properties to the control, but lower levels of nata in frankfurters yielded higher sensory firmness and instrumental hardness values.
24888552	4	79	theme	Sensory	475:481	arg1	results					483:489	Sensory results	475:489	Sensory results	475:489	Sensory results indicated that N10 and N20 had significantly higher firmness scores than N30 and control.
24888552	6	80	theme	%	682:682	arg1	levels					672:677	lower levels	666:677	lower levels (10% to 20%)	666:690	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	6	80	theme	%	682:682	arg1	%					689:689	10% to 20%	680:689	10% to 20%	680:689	Incorporation of nata at lower levels (10% to 20%) did not have detrimental effects on the physicochemical, textural, sensory, and microbiological properties of regular-fat frankfurters.
24888552	9	81	theme	functional	1329:1338	arg1	nata					1352:1355	nata	1352:1355	nata	1352:1355	PRACTICAL APPLICATION We demonstrated the manufacture of quality-improved frankfurters with the inclusion of a high-fiber functional ingredient, nata.
24888552	9	81	theme	functional	1329:1338	arg1	ingredient					1340:1349	a high-fiber functional ingredient	1316:1349	a high-fiber functional ingredient	1316:1349	PRACTICAL APPLICATION We demonstrated the manufacture of quality-improved frankfurters with the inclusion of a high-fiber functional ingredient, nata.
24522808	0	0	theme	cartilage	94:102	arg1	composition					104:114	articular cartilage composition	84:114	articular cartilage composition	84:114	Optimal variable selection for Fourier transform infrared spectroscopic analysis of articular cartilage composition.
24522808	4	1	theme	absorption	519:528	arg1	spectra					530:536	infrared (FT-IR) absorption spectra	502:536	infrared (FT-IR) absorption spectra	502:536	Fourier transform infrared (FT-IR) absorption spectra exhibit detailed information about the biochemical composition of AC.
24522808	8	2	theme	regression	1034:1043	arg1	models					1045:1050	the PLS regression models	1026:1050	the PLS regression models	1026:1050	The optimal variables for the PLS regression models were selected by using backward interval partial least squares and genetic algorithm.
24522808	4	3	theme	FT-IR	512:516	arg1	spectra					530:536	infrared (FT-IR) absorption spectra	502:536	infrared (FT-IR) absorption spectra	502:536	Fourier transform infrared (FT-IR) absorption spectra exhibit detailed information about the biochemical composition of AC.
24522808	9	4	theme	correlation	1149:1159	arg1	coefficients					1161:1172	The linear correlation coefficients	1138:1172	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents	1138:1265	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents were r=0.923 (p<0.001) and r=0.896 (p<0.001), respectively.
24522808	9	4	theme	correlation	1149:1159	arg1	r=0.923					1272:1278	r=0.923	1272:1278	r=0.923	1272:1278	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents were r=0.923 (p<0.001) and r=0.896 (p<0.001), respectively.
24522808	4	5	dep	transform	492:500	arg1	exhibit					538:544	exhibit	538:544	transform infrared (FT-IR) absorption spectra exhibit detailed information about the biochemical composition of AC	492:605	Fourier transform infrared (FT-IR) absorption spectra exhibit detailed information about the biochemical composition of AC.
24522808	0	6	theme	articular	84:92	arg1	composition					104:114	articular cartilage composition	84:114	articular cartilage composition	84:114	Optimal variable selection for Fourier transform infrared spectroscopic analysis of articular cartilage composition.
24522808	4	7	theme	detailed	546:553	arg1	information					555:565	detailed information	546:565	detailed information about the biochemical composition of AC	546:605	Fourier transform infrared (FT-IR) absorption spectra exhibit detailed information about the biochemical composition of AC.
24522808	8	8	theme	PLS	1030:1032	arg1	models					1045:1050	the PLS regression models	1026:1050	the PLS regression models	1026:1050	The optimal variables for the PLS regression models were selected by using backward interval partial least squares and genetic algorithm.
24522808	5	9	theme	FT-IR	633:637	arg1	analysis					639:646	highly specific FT-IR analysis	617:646	highly specific FT-IR analysis for collagen and proteoglycans	617:677	However, highly specific FT-IR analysis for collagen and proteoglycans is challenging.
24522808	10	10	theme	biochemical	1453:1463	arg1	composition					1465:1475	the biochemical composition	1449:1475	the biochemical composition of AC	1449:1481	The results of the study show that variable selection algorithms can significantly improve the PLS regression models when the biochemical composition of AC is predicted.
24522808	10	11	theme	selection	1371:1379	arg1	algorithms					1381:1390	variable selection algorithms	1362:1390	variable selection algorithms	1362:1390	The results of the study show that variable selection algorithms can significantly improve the PLS regression models when the biochemical composition of AC is predicted.
24522808	8	12	theme	optimal	1004:1010	arg1	variables					1012:1020	The optimal variables	1000:1020	The optimal variables for the PLS regression models	1000:1050	The optimal variables for the PLS regression models were selected by using backward interval partial least squares and genetic algorithm.
24522808	7	13	theme	average	977:983	arg1	spectra					991:997	their average FT-IR spectra	971:997	their average FT-IR spectra	971:997	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	10	14	theme	variable	1362:1369	arg1	algorithms					1381:1390	variable selection algorithms	1362:1390	variable selection algorithms	1362:1390	The results of the study show that variable selection algorithms can significantly improve the PLS regression models when the biochemical composition of AC is predicted.
24522808	0	15	theme	composition	104:114	arg1	analysis					72:79	spectroscopic analysis	58:79	spectroscopic analysis of articular cartilage composition	58:114	Optimal variable selection for Fourier transform infrared spectroscopic analysis of articular cartilage composition.
24522808	9	16	theme	linear	1142:1147	arg1	coefficients					1161:1172	The linear correlation coefficients	1138:1172	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents	1138:1265	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents were r=0.923 (p<0.001) and r=0.896 (p<0.001), respectively.
24522808	9	16	theme	linear	1142:1147	arg1	r=0.923					1272:1278	r=0.923	1272:1278	r=0.923	1272:1278	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents were r=0.923 (p<0.001) and r=0.896 (p<0.001), respectively.
24522808	7	17	theme	samples	958:964	arg1	content					923:929	collagen content	914:929	collagen content (n=28)	914:936	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	17	theme	samples	958:964	arg1	n=28					932:935	n=28	932:935	n=28	932:935	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	17	theme	samples	958:964	arg1	n=32					904:907	n=32	904:907	n=32	904:907	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	17	theme	samples	958:964	arg1	content					895:901	the proteoglycan content	878:901	the proteoglycan content (n=32)	878:908	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	6	18	theme	biochemical	748:758	arg1	composition					760:770	the biochemical composition	744:770	the biochemical composition of AC from the FT-IR spectra	744:799	In this study, a chemometric approach to predict the biochemical composition of AC from the FT-IR spectra was investigated.
24522808	4	19	theme	infrared	502:509	arg1	spectra					530:536	infrared (FT-IR) absorption spectra	502:536	infrared (FT-IR) absorption spectra	502:536	Fourier transform infrared (FT-IR) absorption spectra exhibit detailed information about the biochemical composition of AC.
24522808	3	20	theme	progress	456:463	arg1	function					429:436	the function	425:436	the function of the tissue and progress of osteoarthritis	425:481	Characterization of the spatial distribution of these components in AC is important for understanding the function of the tissue and progress of osteoarthritis.
24522808	3	21	theme	components	377:386	arg1	distribution					355:366	the spatial distribution	343:366	the spatial distribution of these components in AC	343:392	Characterization of the spatial distribution of these components in AC is important for understanding the function of the tissue and progress of osteoarthritis.
24522808	10	22	theme	study	1346:1350	arg1	results					1331:1337	The results	1327:1337	The results of the study	1327:1350	The results of the study show that variable selection algorithms can significantly improve the PLS regression models when the biochemical composition of AC is predicted.
24522808	7	23	theme	bovine	941:946	arg1	samples					958:964	bovine cartilage samples	941:964	bovine cartilage samples from their average FT-IR spectra	941:997	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	2	24	theme	biomechanical	283:295	arg1	properties					297:306	unique biomechanical properties	276:306	unique biomechanical properties	276:306	These constituents are inhomogeneously distributed to provide unique biomechanical properties to the tissue.
24522808	7	25	from	spectra	991:997	arg1	content					923:929	collagen content	914:929	collagen content (n=28)	914:936	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	25	from	spectra	991:997	arg1	n=28					932:935	n=28	932:935	n=28	932:935	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	25	from	spectra	991:997	arg1	n=32					904:907	n=32	904:907	n=32	904:907	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	25	from	spectra	991:997	arg1	samples					958:964	bovine cartilage samples	941:964	bovine cartilage samples from their average FT-IR spectra	941:997	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	25	from	spectra	991:997	arg1	content					895:901	the proteoglycan content	878:901	the proteoglycan content (n=32)	878:908	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	0	26	theme	variable	8:15	arg1	Fourier					31:37	Optimal variable selection for Fourier	0:37	Optimal variable selection for Fourier	0:37	Optimal variable selection for Fourier transform infrared spectroscopic analysis of articular cartilage composition.
24522808	3	27	theme	spatial	347:353	arg1	distribution					355:366	the spatial distribution	343:366	the spatial distribution of these components in AC	343:392	Characterization of the spatial distribution of these components in AC is important for understanding the function of the tissue and progress of osteoarthritis.
24522808	8	28	theme	backward	1075:1082	arg1	squares					1107:1113	squares	1107:1113	squares	1107:1113	The optimal variables for the PLS regression models were selected by using backward interval partial least squares and genetic algorithm.
24522808	7	29	theme	cartilage	948:956	arg1	samples					958:964	bovine cartilage samples	941:964	bovine cartilage samples from their average FT-IR spectra	941:997	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	3	30	theme	tissue	445:450	arg1	function					429:436	the function	425:436	the function of the tissue and progress of osteoarthritis	425:481	Characterization of the spatial distribution of these components in AC is important for understanding the function of the tissue and progress of osteoarthritis.
24522808	0	31	theme	Optimal	0:6	arg1	Fourier					31:37	Optimal variable selection for Fourier	0:37	Optimal variable selection for Fourier	0:37	Optimal variable selection for Fourier transform infrared spectroscopic analysis of articular cartilage composition.
24522808	9	32	theme	collagen	1249:1256	arg1	contents					1258:1265	proteoglycan and collagen contents	1232:1265	proteoglycan and collagen contents	1232:1265	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents were r=0.923 (p<0.001) and r=0.896 (p<0.001), respectively.
24522808	7	33	from	content	923:929	arg1	spectra					991:997	their average FT-IR spectra	971:997	their average FT-IR spectra	971:997	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	0	34	theme	for	27:29	arg1	Fourier					31:37	Optimal variable selection for Fourier	0:37	Optimal variable selection for Fourier	0:37	Optimal variable selection for Fourier transform infrared spectroscopic analysis of articular cartilage composition.
24522808	8	35	theme	partial	1093:1099	arg1	squares					1107:1113	squares	1107:1113	squares	1107:1113	The optimal variables for the PLS regression models were selected by using backward interval partial least squares and genetic algorithm.
24522808	5	36	theme	specific	624:631	arg1	analysis					639:646	highly specific FT-IR analysis	617:646	highly specific FT-IR analysis for collagen and proteoglycans	617:677	However, highly specific FT-IR analysis for collagen and proteoglycans is challenging.
24522808	1	37	theme	Articular	117:125	arg1	AC					138:139	AC	138:139	AC	138:139	Articular cartilage (AC) is mainly composed of collagen, proteoglycans, chondrocytes, and water.
24522808	1	37	theme	Articular	117:125	arg1	cartilage					127:135	Articular cartilage	117:135	Articular cartilage (AC)	117:140	Articular cartilage (AC) is mainly composed of collagen, proteoglycans, chondrocytes, and water.
24522808	6	38	theme	chemometric	712:722	arg1	approach					724:731	a chemometric approach	710:731	a chemometric approach to predict the biochemical composition of AC from the FT-IR spectra	710:799	In this study, a chemometric approach to predict the biochemical composition of AC from the FT-IR spectra was investigated.
24522808	0	39	theme	selection	17:25	arg1	Fourier					31:37	Optimal variable selection for Fourier	0:37	Optimal variable selection for Fourier	0:37	Optimal variable selection for Fourier transform infrared spectroscopic analysis of articular cartilage composition.
24522808	7	40	theme	squares	833:839	arg1	regression					847:856	Partial least squares (PLS) regression	819:856	Partial least squares (PLS) regression	819:856	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	8	41	theme	interval	1084:1091	arg1	squares					1107:1113	squares	1107:1113	squares	1107:1113	The optimal variables for the PLS regression models were selected by using backward interval partial least squares and genetic algorithm.
24522808	10	42	theme	PLS	1422:1424	arg1	models					1437:1442	the PLS regression models	1418:1442	the PLS regression models	1418:1442	The results of the study show that variable selection algorithms can significantly improve the PLS regression models when the biochemical composition of AC is predicted.
24522808	4	43	theme	AC	604:605	arg1	composition					589:599	the biochemical composition	573:599	the biochemical composition of AC	573:605	Fourier transform infrared (FT-IR) absorption spectra exhibit detailed information about the biochemical composition of AC.
24522808	3	44	dep	tissue	445:450	arg1	the					441:443	the	441:443	the	441:443	Characterization of the spatial distribution of these components in AC is important for understanding the function of the tissue and progress of osteoarthritis.
24522808	3	45	theme	osteoarthritis	468:481	arg1	progress					456:463	progress	456:463	progress	456:463	Characterization of the spatial distribution of these components in AC is important for understanding the function of the tissue and progress of osteoarthritis.
24522808	3	45	theme	osteoarthritis	468:481	arg1	tissue					445:450	tissue	445:450	tissue	445:450	Characterization of the spatial distribution of these components in AC is important for understanding the function of the tissue and progress of osteoarthritis.
24522808	7	46	theme	Partial	819:825	arg1	PLS					842:844	PLS	842:844	PLS	842:844	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	46	theme	Partial	819:825	arg1	squares					833:839	Partial least squares	819:839	Partial least squares (PLS) regression	819:856	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	8	47	theme	least	1101:1105	arg1	squares					1107:1113	squares	1107:1113	squares	1107:1113	The optimal variables for the PLS regression models were selected by using backward interval partial least squares and genetic algorithm.
24522808	7	48	theme	proteoglycan	882:893	arg1	n=32					904:907	n=32	904:907	n=32	904:907	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	48	theme	proteoglycan	882:893	arg1	content					895:901	the proteoglycan content	878:901	the proteoglycan content (n=32)	878:908	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	0	49	theme	spectroscopic	58:70	arg1	analysis					72:79	spectroscopic analysis	58:79	spectroscopic analysis of articular cartilage composition	58:114	Optimal variable selection for Fourier transform infrared spectroscopic analysis of articular cartilage composition.
24522808	7	50	theme	least	827:831	arg1	PLS					842:844	PLS	842:844	PLS	842:844	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	50	theme	least	827:831	arg1	squares					833:839	Partial least squares	819:839	Partial least squares (PLS) regression	819:856	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	8	51	theme	genetic	1119:1125	arg1	algorithm					1127:1135	genetic algorithm	1119:1135	genetic algorithm	1119:1135	The optimal variables for the PLS regression models were selected by using backward interval partial least squares and genetic algorithm.
24522808	3	52	from	AC	391:392	arg1	Characterization					323:338	Characterization	323:338	Characterization of the spatial distribution of these components in AC	323:392	Characterization of the spatial distribution of these components in AC is important for understanding the function of the tissue and progress of osteoarthritis.
24522808	0	53	dep	transform	39:47	arg1	infrared					49:56	infrared	49:56	transform infrared spectroscopic analysis of articular cartilage composition	39:114	Optimal variable selection for Fourier transform infrared spectroscopic analysis of articular cartilage composition.
24522808	9	54	theme	contents	1258:1265	arg1	values					1222:1227	the biochemical reference and predicted values	1182:1227	values	1222:1227	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents were r=0.923 (p<0.001) and r=0.896 (p<0.001), respectively.
24522808	9	54	theme	contents	1258:1265	arg1	reference					1198:1206	the biochemical reference and predicted values	1182:1227	reference	1198:1206	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents were r=0.923 (p<0.001) and r=0.896 (p<0.001), respectively.
24522808	4	55	theme	biochemical	577:587	arg1	composition					589:599	the biochemical composition	573:599	the biochemical composition of AC	573:605	Fourier transform infrared (FT-IR) absorption spectra exhibit detailed information about the biochemical composition of AC.
24522808	6	56	theme	FT-IR	787:791	arg1	spectra					793:799	the FT-IR spectra	783:799	the FT-IR spectra	783:799	In this study, a chemometric approach to predict the biochemical composition of AC from the FT-IR spectra was investigated.
24522808	7	57	used	used	862:865	arg2	regression					847:856	Partial least squares (PLS) regression	819:856	Partial least squares (PLS) regression	819:856	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	9	58	theme	proteoglycan	1232:1243	arg1	contents					1258:1265	proteoglycan and collagen contents	1232:1265	proteoglycan and collagen contents	1232:1265	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents were r=0.923 (p<0.001) and r=0.896 (p<0.001), respectively.
24522808	2	59	theme	unique	276:281	arg1	properties					297:306	unique biomechanical properties	276:306	unique biomechanical properties	276:306	These constituents are inhomogeneously distributed to provide unique biomechanical properties to the tissue.
24522808	9	60	theme	predicted	1212:1220	arg1	values					1222:1227	the biochemical reference and predicted values	1182:1227	values	1222:1227	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents were r=0.923 (p<0.001) and r=0.896 (p<0.001), respectively.
24522808	9	61	theme	biochemical	1186:1196	arg1	reference					1198:1206	the biochemical reference and predicted values	1182:1227	reference	1198:1206	The linear correlation coefficients between the biochemical reference and predicted values of proteoglycan and collagen contents were r=0.923 (p<0.001) and r=0.896 (p<0.001), respectively.
24522808	3	62	from	distribution	355:366	arg1	AC					391:392	AC	391:392	AC	391:392	Characterization of the spatial distribution of these components in AC is important for understanding the function of the tissue and progress of osteoarthritis.
24522808	6	63	from	spectra	793:799	arg1	composition					760:770	the biochemical composition	744:770	the biochemical composition of AC from the FT-IR spectra	744:799	In this study, a chemometric approach to predict the biochemical composition of AC from the FT-IR spectra was investigated.
24522808	7	64	theme	collagen	914:921	arg1	content					923:929	collagen content	914:929	collagen content (n=28)	914:936	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	64	theme	collagen	914:921	arg1	n=28					932:935	n=28	932:935	n=28	932:935	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	3	65	theme	distribution	355:366	arg1	Characterization					323:338	Characterization	323:338	Characterization of the spatial distribution of these components in AC	323:392	Characterization of the spatial distribution of these components in AC is important for understanding the function of the tissue and progress of osteoarthritis.
24522808	6	66	theme	AC	775:776	arg1	composition					760:770	the biochemical composition	744:770	the biochemical composition of AC from the FT-IR spectra	744:799	In this study, a chemometric approach to predict the biochemical composition of AC from the FT-IR spectra was investigated.
24522808	3	67	from	Characterization	323:338	arg1	AC					391:392	AC	391:392	AC	391:392	Characterization of the spatial distribution of these components in AC is important for understanding the function of the tissue and progress of osteoarthritis.
24522808	10	68	theme	regression	1426:1435	arg1	models					1437:1442	the PLS regression models	1418:1442	the PLS regression models	1418:1442	The results of the study show that variable selection algorithms can significantly improve the PLS regression models when the biochemical composition of AC is predicted.
24522808	7	69	from	content	895:901	arg1	spectra					991:997	their average FT-IR spectra	971:997	their average FT-IR spectra	971:997	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	7	70	theme	FT-IR	985:989	arg1	spectra					991:997	their average FT-IR spectra	971:997	their average FT-IR spectra	971:997	Partial least squares (PLS) regression was used to predict the proteoglycan content (n=32) and collagen content (n=28) of bovine cartilage samples from their average FT-IR spectra.
24522808	10	71	theme	AC	1480:1481	arg1	composition					1465:1475	the biochemical composition	1449:1475	the biochemical composition of AC	1449:1481	The results of the study show that variable selection algorithms can significantly improve the PLS regression models when the biochemical composition of AC is predicted.
24059748	0	0	theme	whole	83:87	arg1	spp					116:118	extruded whole grain red sorghum (sorghum spp)	74:119	extruded whole grain red sorghum (sorghum spp)	74:119	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	3	1	theme	good	963:966	arg1	properties					968:977	good properties	963:977	good properties	963:977	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	3	2	theme	164 °C-19	883:891	arg1	%					892:892	%	892:892	%	892:892	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	1	3	from	effects	146:152	arg1	properties					296:305	textural and physicochemical properties	267:305	textural and physicochemical properties of red sorghum extrudates	267:331	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	3	4	theme	M.	664:665	arg1	solubility					673:682	200 °C-14%M. Water solubility	654:682	200 °C-14%M. Water solubility	654:682	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	0	5	theme	extruded	74:81	arg1	spp					116:118	extruded whole grain red sorghum (sorghum spp)	74:119	extruded whole grain red sorghum (sorghum spp)	74:119	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	2	6	theme	164 °C-14	524:532	arg1	%					533:533	%	533:533	%	533:533	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	3	7	theme	%	892:892	arg1	Extrusion					896:904	164 °C-19%M. Extrusion	883:904	164 °C-19%M. Extrusion	883:904	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	1	8	theme	sorghum	314:320	arg1	extrudates					322:331	red sorghum extrudates	310:331	red sorghum extrudates	310:331	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	3	9	theme	Water	667:671	arg1	solubility					673:682	200 °C-14%M. Water solubility	654:682	200 °C-14%M. Water solubility	654:682	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	0	10	theme	red	95:97	arg1	spp					116:118	extruded whole grain red sorghum (sorghum spp)	74:119	extruded whole grain red sorghum (sorghum spp)	74:119	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	0	11	from	Effects	0:6	arg1	properties					60:69	physical and nutritional properties	35:69	physical and nutritional properties of extruded whole grain red sorghum (sorghum spp)	35:119	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	1	12	theme	extrudates	322:331	arg1	properties					296:305	textural and physicochemical properties	267:305	textural and physicochemical properties of red sorghum extrudates	267:331	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	3	13	theme	greatest	814:821	arg1	degree					831:836	the greatest cooking degree	810:836	the greatest cooking degree	810:836	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	3	14	theme	200 °C-14	654:662	arg1	%					663:663	%	663:663	%	663:663	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	0	15	theme	grain	89:93	arg1	spp					116:118	extruded whole grain red sorghum (sorghum spp)	74:119	extruded whole grain red sorghum (sorghum spp)	74:119	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	4	16	theme	significant	1028:1038	arg1	differences					1040:1050	statistically significant differences	1014:1050	statistically significant differences	1014:1050	Proximal composition did not show statistically significant differences with raw sample.
24059748	5	17	theme	%	1138:1138	arg1	increase					1140:1147	a 31% increase	1134:1147	a 31% increase in protein digestibility	1134:1172	Extruded sample showed a 25.4% reduction of available lysine and a 31% increase in protein digestibility.
24059748	1	18	theme	grits	205:209	arg1	sample					237:242	g/100 g sample	229:242	g/100 g sample	229:242	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	1	18	theme	grits	205:209	arg1	content					220:226	grits moisture content	205:226	grits moisture content (g/100 g sample) (%M: 14, 16.5, 19)	205:262	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	1	19	theme	whole	334:338	arg1	flour					346:350	whole grain flour	334:350	whole grain flour	334:350	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	0	20	theme	sorghum	99:105	arg1	spp					116:118	extruded whole grain red sorghum (sorghum spp)	74:119	extruded whole grain red sorghum (sorghum spp)	74:119	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	3	21	theme	182 °C-14	909:917	arg1	%					918:918	%	918:918	%	918:918	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	1	22	theme	grain	340:344	arg1	flour					346:350	whole grain flour	334:350	whole grain flour	334:350	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	3	23	theme	expanded	941:948	arg1	product					950:956	an expanded product	938:956	an expanded product with good properties	938:977	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	3	24	theme	M.	893:894	arg1	Extrusion					896:904	164 °C-19%M. Extrusion	883:904	164 °C-19%M. Extrusion	883:904	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	5	25	theme	protein	1152:1158	arg1	digestibility					1160:1172	protein digestibility	1152:1172	protein digestibility	1152:1172	Extruded sample showed a 25.4% reduction of available lysine and a 31% increase in protein digestibility.
24059748	3	26	with	product	950:956	arg1	properties					968:977	good properties	963:977	good properties	963:977	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	3	27	with	related	719:725	arg1	T					732:732	T	732:732	T	732:732	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	3	27	with	related	719:725	arg1	observation					768:778	M. Microscopic observation	753:778	M. Microscopic observation of the samples	753:793	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	1	28	theme	moisture	211:218	arg1	sample					237:242	g/100 g sample	229:242	g/100 g sample	229:242	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	1	28	theme	moisture	211:218	arg1	content					220:226	grits moisture content	205:226	grits moisture content (g/100 g sample) (%M: 14, 16.5, 19)	205:262	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	3	29	theme	%	862:862	arg1	M					863:863	200 °C-4%M	854:863	200 °C-4%M	854:863	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	5	30	theme	lysine	1123:1128	arg1	reduction					1100:1108	a 25.4% reduction	1092:1108	a 25.4% reduction of available lysine	1092:1128	Extruded sample showed a 25.4% reduction of available lysine and a 31% increase in protein digestibility.
24059748	5	30	theme	lysine	1123:1128	arg1	increase					1140:1147	a 31% increase	1134:1147	a 31% increase in protein digestibility	1134:1172	Extruded sample showed a 25.4% reduction of available lysine and a 31% increase in protein digestibility.
24059748	1	31	theme	extrusion	157:165	arg1	T					180:180	T	180:180	T	180:180	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	1	31	theme	extrusion	157:165	arg1	temperature					167:177	extrusion temperature	157:177	extrusion temperature (T: 164, 182, 200 °C)	157:199	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	0	32	theme	conditions	21:30	arg1	Effects					0:6	Effects	0:6	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).	0:120	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	1	33	theme	content	220:226	arg1	effects					146:152	the effects	142:152	the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates	142:331	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	0	34	theme	spp	116:118	arg1	properties					60:69	physical and nutritional properties	35:69	physical and nutritional properties of extruded whole grain red sorghum (sorghum spp)	35:119	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	3	35	theme	%	663:663	arg1	solubility					673:682	200 °C-14%M. Water solubility	654:682	200 °C-14%M. Water solubility	654:682	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	2	36	theme	%	533:533	arg1	M					534:534	164 °C-14%M	524:534	164 °C-14%M	524:534	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	1	37	theme	temperature	167:177	arg1	effects					146:152	the effects	142:152	the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates	142:331	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	0	38	theme	extrusion	11:19	arg1	conditions					21:30	extrusion conditions	11:30	extrusion conditions	11:30	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	3	39	theme	specific	589:596	arg1	volume					598:603	specific volume	589:603	specific volume	589:603	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	0	40	theme	sorghum	108:114	arg1	spp					116:118	extruded whole grain red sorghum (sorghum spp)	74:119	extruded whole grain red sorghum (sorghum spp)	74:119	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	5	41	theme	31	1136:1137	arg1	%					1138:1138	%	1138:1138	%	1138:1138	Extruded sample showed a 25.4% reduction of available lysine and a 31% increase in protein digestibility.
24059748	3	42	theme	M.	753:754	arg1	observation					768:778	M. Microscopic observation	753:778	M. Microscopic observation of the samples	753:793	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	2	43	from	M	576:576	arg1	hardness					554:561	sensorial hardness	544:561	sensorial hardness at 164 °C-19%M	544:576	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	0	44	theme	physical	35:42	arg1	properties					60:69	physical and nutritional properties	35:69	physical and nutritional properties of extruded whole grain red sorghum (sorghum spp)	35:119	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	1	45	dep	M	247:247	arg1	19					260:261	19	260:261	19	260:261	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	3	46	theme	%	918:918	arg1	M					919:919	182 °C-14%M	909:919	182 °C-14%M	909:919	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	3	47	theme	Microscopic	756:766	arg1	observation					768:778	M. Microscopic observation	753:778	M. Microscopic observation of the samples	753:793	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	5	48	theme	%	1098:1098	arg1	reduction					1100:1108	a 25.4% reduction	1092:1108	a 25.4% reduction of available lysine	1092:1128	Extruded sample showed a 25.4% reduction of available lysine and a 31% increase in protein digestibility.
24059748	1	49	theme	g	235:235	arg1	sample					237:242	g/100 g sample	229:242	g/100 g sample	229:242	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	1	49	theme	g	235:235	arg1	content					220:226	grits moisture content	205:226	grits moisture content (g/100 g sample) (%M: 14, 16.5, 19)	205:262	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	2	50	theme	higher	415:420	arg1	values					422:427	The higher values	411:427	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36)	411:505	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	1	51	theme	textural	267:274	arg1	properties					296:305	textural and physicochemical properties	267:305	textural and physicochemical properties of red sorghum extrudates	267:331	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	2	52	theme	%	575:575	arg1	M					576:576	164 °C-19%M	566:576	164 °C-19%M	566:576	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	5	53	from	reduction	1100:1108	arg1	digestibility					1160:1172	protein digestibility	1152:1172	protein digestibility	1152:1172	Extruded sample showed a 25.4% reduction of available lysine and a 31% increase in protein digestibility.
24059748	0	54	theme	nutritional	48:58	arg1	properties					60:69	physical and nutritional properties	35:69	physical and nutritional properties of extruded whole grain red sorghum (sorghum spp)	35:119	Effects of extrusion conditions on physical and nutritional properties of extruded whole grain red sorghum (sorghum spp).
24059748	3	55	theme	samples	787:793	arg1	observation					768:778	M. Microscopic observation	753:778	M. Microscopic observation of the samples	753:793	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	2	56	theme	164 °C-19	566:574	arg1	%					575:575	%	575:575	%	575:575	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	1	57	dep	content	220:226	arg1	M					247:247	%M	246:247	%M	246:247	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	3	58	theme	highest	610:616	arg1	value					618:622	the highest value	606:622	the highest value (10.41 cm³/g)	606:636	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	3	58	theme	highest	610:616	arg1	10.41 cm³/g					625:635	10.41 cm³/g	625:635	10.41 cm³/g	625:635	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	2	59	theme	specific	433:440	arg1	1006.98 J/g					473:483	1006.98 J/g	473:483	1006.98 J/g	473:483	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	2	59	theme	specific	433:440	arg1	consumption					460:470	specific mechanical energy consumption	433:470	specific mechanical energy consumption (1006.98 J/g)	433:484	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	1	60	theme	physicochemical	280:294	arg1	properties					296:305	textural and physicochemical properties	267:305	textural and physicochemical properties of red sorghum extrudates	267:331	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	3	61	theme	water	688:692	arg1	absorption					694:703	water absorption	688:703	water absorption	688:703	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	2	62	theme	energy	453:458	arg1	1006.98 J/g					473:483	1006.98 J/g	473:483	1006.98 J/g	473:483	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	2	62	theme	energy	453:458	arg1	consumption					460:470	specific mechanical energy consumption	433:470	specific mechanical energy consumption (1006.98 J/g)	433:484	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	2	63	theme	sensorial	544:552	arg1	hardness					554:561	sensorial hardness	544:561	sensorial hardness at 164 °C-19%M	544:576	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	1	64	dep	T	180:180	arg1	164					183:185	164	183:185	164	183:185	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	1	64	dep	T	180:180	arg1	200 °C					193:198	200 °C	193:198	200 °C	193:198	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	1	65	theme	factorial	380:388	arg1	design					403:408	a factorial experimental design	378:408	a factorial experimental design	378:408	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	2	66	theme	mechanical	442:451	arg1	1006.98 J/g					473:483	1006.98 J/g	473:483	1006.98 J/g	473:483	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	2	66	theme	mechanical	442:451	arg1	consumption					460:470	specific mechanical energy consumption	433:470	specific mechanical energy consumption (1006.98 J/g)	433:484	The higher values for specific mechanical energy consumption (1006.98 J/g) and expansion (3.36) were obtained at 164 °C-14%M and for sensorial hardness at 164 °C-19%M.
24059748	1	67	theme	experimental	390:401	arg1	design					403:408	a factorial experimental design	378:408	a factorial experimental design	378:408	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	3	68	theme	cooking	823:829	arg1	degree					831:836	the greatest cooking degree	810:836	the greatest cooking degree	810:836	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	1	69	theme	red	310:312	arg1	extrudates					322:331	red sorghum extrudates	310:331	red sorghum extrudates	310:331	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	3	70	theme	200 °C-4	854:861	arg1	%					862:862	%	862:862	%	862:862	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	5	71	theme	available	1113:1121	arg1	lysine					1123:1128	available lysine	1113:1128	available lysine	1113:1128	Extruded sample showed a 25.4% reduction of available lysine and a 31% increase in protein digestibility.
24059748	4	72	theme	raw	1057:1059	arg1	sample					1061:1066	raw sample	1057:1066	raw sample	1057:1066	Proximal composition did not show statistically significant differences with raw sample.
24059748	3	73	dep	M	919:919	arg1	allows					921:926	allows	921:926	allows obtaining an expanded product with good properties	921:977	While for specific volume, the highest value (10.41 cm³/g) was obtained at 200 °C-14%M. Water solubility and water absorption were directly related with T and inversely with M. Microscopic observation of the samples indicates that the greatest cooking degree was obtained at 200 °C-4%M and the lowest at 164 °C-19%M. Extrusion at 182 °C-14%M allows obtaining an expanded product with good properties.
24059748	1	74	theme	%	246:246	arg1	M					247:247	%M	246:247	%M	246:247	In order to analyze the effects of extrusion temperature (T: 164, 182, 200 °C) and grits moisture content (g/100 g sample) (%M: 14, 16.5, 19) on textural and physicochemical properties of red sorghum extrudates, whole grain flour was extruded according to a factorial experimental design.
24059748	5	75	from	increase	1140:1147	arg1	digestibility					1160:1172	protein digestibility	1152:1172	protein digestibility	1152:1172	Extruded sample showed a 25.4% reduction of available lysine and a 31% increase in protein digestibility.
24059748	4	76	theme	Proximal	980:987	arg1	composition					989:999	Proximal composition	980:999	Proximal composition	980:999	Proximal composition did not show statistically significant differences with raw sample.
24059748	5	77	theme	Extruded	1069:1076	arg1	sample					1078:1083	Extruded sample	1069:1083	Extruded sample	1069:1083	Extruded sample showed a 25.4% reduction of available lysine and a 31% increase in protein digestibility.
27287318	10	0	contain	has	1732:1734	arg2	structure					1746:1754	a modular structure	1736:1754	a modular structure with an enzymatically active domain and a cell wall binding domain	1736:1821	This endolysin has a modular structure with an enzymatically active domain and a cell wall binding domain.
27287318	10	0	contain	has	1732:1734	arg1	endolysin					1722:1730	This endolysin	1717:1730	This endolysin	1717:1730	This endolysin has a modular structure with an enzymatically active domain and a cell wall binding domain.
27287318	6	1	theme	enzymatic	1108:1116	arg1	activity					1118:1125	enzymatic activity	1108:1125	enzymatic activity of pH 8	1108:1133	Gp110 is a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance.
27287318	3	2	theme	Gram-negative	530:542	arg1	species					544:550	Gram-negative species	530:550	Gram-negative species	530:550	Despite the wealth of applications relying on the use of endolysin, little is known about the enzymatic properties of these enzymes, especially in the case of endolysins of bacteriophages infecting Gram-negative species.
27287318	5	3	from	bacteria	995:1002	arg1	peptidoglycan					962:974	peptidoglycan	962:974	peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity)	962:1054	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	15	4	theme	known	2466:2470	arg1	structures					2472:2481	known structures	2466:2481	known structures of enzymes with the same peptidoglycan cleavage specificity	2466:2541	Three-dimensional structure predictions for Gp110 suggest a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique.
27287318	5	5	theme	Salmonella	700:709	arg1	Gp110					736:740	Gp110	736:740	Gp110	736:740	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	5	5	theme	Salmonella	700:709	arg1	endolysin					725:733	a novel Salmonella bacteriophage endolysin	692:733	a novel Salmonella bacteriophage endolysin	692:733	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	2	6	theme	biotechnological	308:323	arg1	tools					325:329	biotechnological tools	308:329	biotechnological tools	308:329	Current research focuses on their potential applications in medicine, in food conservation, and as biotechnological tools.
27287318	16	7	theme	thermal	2627:2633	arg1	resistance					2635:2644	increased thermal resistance	2617:2644	increased thermal resistance	2617:2644	All of these features, combined with increased thermal resistance, make Gp110 an attractive candidate for engineering novel endolysin-based antibacterials.
27287318	5	8	theme	cleavage	661:668	arg1	determination					675:687	cleavage site determination	661:687	cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity)	661:1054	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	16	9	theme	increased	2617:2625	arg1	resistance					2635:2644	increased thermal resistance	2617:2644	increased thermal resistance	2617:2644	All of these features, combined with increased thermal resistance, make Gp110 an attractive candidate for engineering novel endolysin-based antibacterials.
27287318	11	10	theme	other	1885:1889	arg1	endolysins					1891:1900	all other endolysins	1881:1900	all other endolysins previously characterized using the same methods	1881:1948	The enzymatic activity of this endolysin exceeds that of all other endolysins previously characterized using the same methods.
27287318	5	11	dep	364-fold	1030:1037	arg1	higher					1039:1044	higher	1039:1044	higher	1039:1044	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	5	11	dep	364-fold	1030:1037	arg1	to					1027:1028	to	1027:1028	to	1027:1028	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	1	12	theme	Bacteriophage-encoded	102:122	arg1	enzymes					154:160	highly diverse enzymes	139:160	highly diverse enzymes that cleave the bacterial peptidoglycan layer	139:206	UNLABELLED Bacteriophage-encoded endolysins are highly diverse enzymes that cleave the bacterial peptidoglycan layer.
27287318	1	12	theme	Bacteriophage-encoded	102:122	arg1	endolysins					124:133	UNLABELLED Bacteriophage-encoded endolysins	91:133	UNLABELLED Bacteriophage-encoded endolysins	91:133	UNLABELLED Bacteriophage-encoded endolysins are highly diverse enzymes that cleave the bacterial peptidoglycan layer.
27287318	10	13	theme	wall	1803:1806	arg1	domain					1816:1821	a cell wall binding domain	1796:1821	a cell wall binding domain	1796:1821	This endolysin has a modular structure with an enzymatically active domain and a cell wall binding domain.
27287318	5	14	theme	higher	856:861	arg1	activity					872:879	a higher specific activity	854:879	a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity)	854:1054	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	5	14	theme	higher	856:861	arg1	U/μM					889:892	34,240 U/μM	882:892	34,240 U/μM	882:892	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	1	15	theme	diverse	146:152	arg1	enzymes					154:160	highly diverse enzymes	139:160	highly diverse enzymes that cleave the bacterial peptidoglycan layer	139:206	UNLABELLED Bacteriophage-encoded endolysins are highly diverse enzymes that cleave the bacterial peptidoglycan layer.
27287318	1	15	theme	diverse	146:152	arg1	endolysins					124:133	UNLABELLED Bacteriophage-encoded endolysins	91:133	UNLABELLED Bacteriophage-encoded endolysins	91:133	UNLABELLED Bacteriophage-encoded endolysins are highly diverse enzymes that cleave the bacterial peptidoglycan layer.
27287318	8	16	theme	peptide	1506:1512	arg1	compositions					1519:1530	various peptide stem compositions	1498:1530	various peptide stem compositions	1498:1530	Gp110 is active against directly cross-linked peptidoglycans with various peptide stem compositions, making it an attractive enzyme for developing novel antimicrobial agents.
27287318	14	17	theme	endolysins	2295:2304	arg1	activities					2281:2290	the enzymatic activities	2267:2290	the enzymatic activities of endolysins, which mostly are inferred by sequence similarities	2267:2356	This experimentally verified activity allows better classification and understanding of the enzymatic activities of endolysins, which mostly are inferred by sequence similarities.
27287318	7	18	theme	N-acetylglucosamine	1402:1420	arg1	residues					1422:1429	N-acetylglucosamine residues	1402:1429	N-acetylglucosamine residues	1402:1429	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	14	19	theme	activities	2281:2290	arg1	classification					2231:2244	classification	2231:2244	classification	2231:2244	This experimentally verified activity allows better classification and understanding of the enzymatic activities of endolysins, which mostly are inferred by sequence similarities.
27287318	14	19	theme	activities	2281:2290	arg1	understanding					2250:2262	understanding	2250:2262	understanding	2250:2262	This experimentally verified activity allows better classification and understanding of the enzymatic activities of endolysins, which mostly are inferred by sequence similarities.
27287318	8	20	with	peptidoglycans	1478:1491	arg1	compositions					1519:1530	various peptide stem compositions	1498:1530	various peptide stem compositions	1498:1530	Gp110 is active against directly cross-linked peptidoglycans with various peptide stem compositions, making it an attractive enzyme for developing novel antimicrobial agents.
27287318	5	21	theme	endolysin	725:733	arg1	determination					675:687	cleavage site determination	661:687	cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity)	661:1054	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	5	21	theme	endolysin	725:733	arg1	analysis					648:655	the biochemical analysis	632:655	the biochemical analysis	632:655	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	15	22	theme	peptidoglycan	2508:2520	arg1	specificity					2531:2541	the same peptidoglycan cleavage specificity	2499:2541	the same peptidoglycan cleavage specificity	2499:2541	Three-dimensional structure predictions for Gp110 suggest a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique.
27287318	5	23	theme	characterized	922:934	arg1	endolysins					936:945	14 previously characterized endolysins	908:945	14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity)	908:1054	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	5	24	theme	active	947:952	arg1	endolysins					936:945	14 previously characterized endolysins	908:945	14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity)	908:1054	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	5	25	dep	comprises	749:757	arg1	pfam11860					815:823	pfam11860	815:823	pfam11860	815:823	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	0	26	theme	Potent	55:60	arg1	Activity					81:88	Potent N-Acetylmuramidase Activity	55:88	Potent N-Acetylmuramidase Activity	55:88	DUF3380 Domain from a Salmonella Phage Endolysin Shows Potent N-Acetylmuramidase Activity.
27287318	14	27	theme	enzymatic	2271:2279	arg1	activities					2281:2290	the enzymatic activities	2267:2290	the enzymatic activities of endolysins, which mostly are inferred by sequence similarities	2267:2356	This experimentally verified activity allows better classification and understanding of the enzymatic activities of endolysins, which mostly are inferred by sequence similarities.
27287318	7	28	theme	mass	1251:1254	arg1	spectrometry					1256:1267	mass spectrometry	1251:1267	mass spectrometry	1251:1267	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	1	29	theme	peptidoglycan	188:200	arg1	layer					202:206	the bacterial peptidoglycan layer	174:206	the bacterial peptidoglycan layer	174:206	UNLABELLED Bacteriophage-encoded endolysins are highly diverse enzymes that cleave the bacterial peptidoglycan layer.
27287318	13	30	theme	peptide	2152:2158	arg1	compositions					2165:2176	various peptide stem compositions	2144:2176	various peptide stem compositions	2144:2176	We report that DUF3380 has N-acetylmuramidase activity against directly cross-linked peptidoglycans with various peptide stem compositions.
27287318	5	31	theme	biochemical	636:646	arg1	analysis					648:655	the biochemical analysis	632:655	the biochemical analysis	632:655	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	8	32	link	cross-linked	1465:1476	arg1	peptidoglycans					1478:1491	directly cross-linked peptidoglycans	1456:1491	directly cross-linked peptidoglycans with various peptide stem compositions	1456:1530	Gp110 is active against directly cross-linked peptidoglycans with various peptide stem compositions, making it an attractive enzyme for developing novel antimicrobial agents.
27287318	0	33	from	Endolysin	39:47	arg1	Domain					8:13	DUF3380 Domain	0:13	DUF3380 Domain from a Salmonella Phage Endolysin	0:47	DUF3380 Domain from a Salmonella Phage Endolysin Shows Potent N-Acetylmuramidase Activity.
27287318	3	34	theme	enzymes	456:462	arg1	properties					436:445	the enzymatic properties	422:445	the enzymatic properties of these enzymes	422:462	Despite the wealth of applications relying on the use of endolysin, little is known about the enzymatic properties of these enzymes, especially in the case of endolysins of bacteriophages infecting Gram-negative species.
27287318	5	35	theme	unknown	788:794	arg1	function					796:803	unknown function	788:803	unknown function	788:803	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	9	36	theme	functional	1632:1641	arg1	characterization					1659:1674	the functional and biochemical characterization	1628:1674	the functional and biochemical characterization of the Salmonella phage endolysin Gp110	1628:1714	IMPORTANCE We report the functional and biochemical characterization of the Salmonella phage endolysin Gp110.
27287318	6	37	theme	optimal	1094:1100	arg1	pH					1102:1103	an optimal pH	1091:1103	an optimal pH of enzymatic activity of pH 8	1091:1133	Gp110 is a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance.
27287318	0	38	theme	N-Acetylmuramidase	62:79	arg1	Activity					81:88	Potent N-Acetylmuramidase Activity	55:88	Potent N-Acetylmuramidase Activity	55:88	DUF3380 Domain from a Salmonella Phage Endolysin Shows Potent N-Acetylmuramidase Activity.
27287318	0	39	theme	DUF3380	0:6	arg1	Domain					8:13	DUF3380 Domain	0:13	DUF3380 Domain from a Salmonella Phage Endolysin	0:47	DUF3380 Domain from a Salmonella Phage Endolysin Shows Potent N-Acetylmuramidase Activity.
27287318	9	40	theme	biochemical	1647:1657	arg1	characterization					1659:1674	the functional and biochemical characterization	1628:1674	the functional and biochemical characterization of the Salmonella phage endolysin Gp110	1628:1714	IMPORTANCE We report the functional and biochemical characterization of the Salmonella phage endolysin Gp110.
27287318	10	41	theme	active	1778:1783	arg1	domain					1785:1790	an enzymatically active domain	1761:1790	an enzymatically active domain	1761:1790	This endolysin has a modular structure with an enzymatically active domain and a cell wall binding domain.
27287318	7	42	theme	liquid	1199:1204	arg1	RP-HPLC					1222:1228	RP-HPLC	1222:1228	RP-HPLC	1222:1228	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	7	42	theme	liquid	1199:1204	arg1	chromatography					1206:1219	Reverse-phase high-performance liquid chromatography	1168:1219	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry	1168:1267	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	8	43	dep	it	1540:1541	arg1	enzyme					1557:1562	an attractive enzyme	1543:1562	it an attractive enzyme for developing novel antimicrobial agents	1540:1604	Gp110 is active against directly cross-linked peptidoglycans with various peptide stem compositions, making it an attractive enzyme for developing novel antimicrobial agents.
27287318	5	44	theme	Gram-negative	981:993	arg1	bacteria					995:1002	Gram-negative bacteria	981:1002	Gram-negative bacteria	981:1002	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	6	45	theme	modular	1068:1074	arg1	endolysin					1076:1084	a modular endolysin	1066:1084	a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance	1066:1165	Gp110 is a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance.
27287318	6	45	theme	modular	1068:1074	arg1	Gp110					1057:1061	Gp110	1057:1061	Gp110	1057:1061	Gp110 is a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance.
27287318	3	46	theme	enzymatic	426:434	arg1	properties					436:445	the enzymatic properties	422:445	the enzymatic properties of these enzymes	422:462	Despite the wealth of applications relying on the use of endolysin, little is known about the enzymatic properties of these enzymes, especially in the case of endolysins of bacteriophages infecting Gram-negative species.
27287318	2	47	theme	Current	209:215	arg1	research					217:224	Current research	209:224	Current research	209:224	Current research focuses on their potential applications in medicine, in food conservation, and as biotechnological tools.
27287318	0	48	theme	Salmonella	22:31	arg1	Endolysin					39:47	a Salmonella Phage Endolysin	20:47	a Salmonella Phage Endolysin	20:47	DUF3380 Domain from a Salmonella Phage Endolysin Shows Potent N-Acetylmuramidase Activity.
27287318	7	49	theme	Reverse-phase	1168:1180	arg1	RP-HPLC					1222:1228	RP-HPLC	1222:1228	RP-HPLC	1222:1228	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	7	49	theme	Reverse-phase	1168:1180	arg1	chromatography					1206:1219	Reverse-phase high-performance liquid chromatography	1168:1219	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry	1168:1267	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	9	50	theme	Salmonella	1683:1692	arg1	Gp110					1710:1714	the Salmonella phage endolysin Gp110	1679:1714	the Salmonella phage endolysin Gp110	1679:1714	IMPORTANCE We report the functional and biochemical characterization of the Salmonella phage endolysin Gp110.
27287318	4	51	theme	Automated	553:561	arg1	annotations					570:580	Automated genome annotations	553:580	Automated genome annotations	553:580	Automated genome annotations therefore remain to be confirmed.
27287318	10	52	theme	cell	1798:1801	arg1	domain					1816:1821	a cell wall binding domain	1796:1821	a cell wall binding domain	1796:1821	This endolysin has a modular structure with an enzymatically active domain and a cell wall binding domain.
27287318	12	53	theme	unknown	1963:1969	arg1	function					1971:1978	unknown function	1963:1978	unknown function	1963:1978	A domain of unknown function (DUF3380) is responsible for this high enzymatic activity.
27287318	13	54	link	cross-linked	2111:2122	arg1	peptidoglycans					2124:2137	directly cross-linked peptidoglycans	2102:2137	directly cross-linked peptidoglycans with various peptide stem compositions	2102:2176	We report that DUF3380 has N-acetylmuramidase activity against directly cross-linked peptidoglycans with various peptide stem compositions.
27287318	9	55	theme	endolysin	1700:1708	arg1	Gp110					1710:1714	the Salmonella phage endolysin Gp110	1679:1714	the Salmonella phage endolysin Gp110	1679:1714	IMPORTANCE We report the functional and biochemical characterization of the Salmonella phage endolysin Gp110.
27287318	13	56	theme	various	2144:2150	arg1	compositions					2165:2176	various peptide stem compositions	2144:2176	various peptide stem compositions	2144:2176	We report that DUF3380 has N-acetylmuramidase activity against directly cross-linked peptidoglycans with various peptide stem compositions.
27287318	11	57	theme	same	1937:1940	arg1	methods					1942:1948	the same methods	1933:1948	the same methods	1933:1948	The enzymatic activity of this endolysin exceeds that of all other endolysins previously characterized using the same methods.
27287318	15	58	theme	enzymes	2486:2492	arg1	structures					2472:2481	known structures	2466:2481	known structures of enzymes with the same peptidoglycan cleavage specificity	2466:2541	Three-dimensional structure predictions for Gp110 suggest a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique.
27287318	6	59	with	endolysin	1076:1084	arg1	resistance					1156:1165	elevated thermal resistance	1139:1165	elevated thermal resistance	1139:1165	Gp110 is a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance.
27287318	6	59	with	endolysin	1076:1084	arg1	pH					1102:1103	an optimal pH	1091:1103	an optimal pH of enzymatic activity of pH 8	1091:1133	Gp110 is a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance.
27287318	7	60	theme	lysozyme	1313:1320	arg1	activity					1323:1330	N-acetylmuramidase (lysozyme) activity	1293:1330	N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues	1293:1429	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	6	61	theme	activity	1118:1125	arg1	resistance					1156:1165	elevated thermal resistance	1139:1165	elevated thermal resistance	1139:1165	Gp110 is a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance.
27287318	6	61	theme	activity	1118:1125	arg1	pH					1102:1103	an optimal pH	1091:1103	an optimal pH of enzymatic activity of pH 8	1091:1133	Gp110 is a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance.
27287318	8	62	theme	various	1498:1504	arg1	compositions					1519:1530	various peptide stem compositions	1498:1530	various peptide stem compositions	1498:1530	Gp110 is active against directly cross-linked peptidoglycans with various peptide stem compositions, making it an attractive enzyme for developing novel antimicrobial agents.
27287318	7	63	theme	N-acetylmuramidase	1293:1310	arg1	activity					1323:1330	N-acetylmuramidase (lysozyme) activity	1293:1330	N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues	1293:1429	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	10	64	theme	binding	1808:1814	arg1	domain					1816:1821	a cell wall binding domain	1796:1821	a cell wall binding domain	1796:1821	This endolysin has a modular structure with an enzymatically active domain and a cell wall binding domain.
27287318	10	65	with	structure	1746:1754	arg1	domain					1785:1790	an enzymatically active domain	1761:1790	an enzymatically active domain	1761:1790	This endolysin has a modular structure with an enzymatically active domain and a cell wall binding domain.
27287318	10	65	with	structure	1746:1754	arg1	domain					1816:1821	a cell wall binding domain	1796:1821	a cell wall binding domain	1796:1821	This endolysin has a modular structure with an enzymatically active domain and a cell wall binding domain.
27287318	5	66	theme	novel	694:698	arg1	Gp110					736:740	Gp110	736:740	Gp110	736:740	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	5	66	theme	novel	694:698	arg1	endolysin					725:733	a novel Salmonella bacteriophage endolysin	692:733	a novel Salmonella bacteriophage endolysin	692:733	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	13	67	with	peptidoglycans	2124:2137	arg1	compositions					2165:2176	various peptide stem compositions	2144:2176	various peptide stem compositions	2144:2176	We report that DUF3380 has N-acetylmuramidase activity against directly cross-linked peptidoglycans with various peptide stem compositions.
27287318	14	68	theme	sequence	2336:2343	arg1	similarities					2345:2356	sequence similarities	2336:2356	sequence similarities	2336:2356	This experimentally verified activity allows better classification and understanding of the enzymatic activities of endolysins, which mostly are inferred by sequence similarities.
27287318	5	69	theme	site	670:673	arg1	determination					675:687	cleavage site determination	661:687	cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity)	661:1054	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	15	70	from	that	2458:2461	arg1	fold					2419:2422	a fold	2417:2422	a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique	2417:2577	Three-dimensional structure predictions for Gp110 suggest a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique.
27287318	15	70	from	that	2458:2461	arg1	different					2443:2451	different	2443:2451	different	2443:2451	Three-dimensional structure predictions for Gp110 suggest a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique.
27287318	8	71	theme	stem	1514:1517	arg1	compositions					1519:1530	various peptide stem compositions	1498:1530	various peptide stem compositions	1498:1530	Gp110 is active against directly cross-linked peptidoglycans with various peptide stem compositions, making it an attractive enzyme for developing novel antimicrobial agents.
27287318	6	72	theme	thermal	1148:1154	arg1	resistance					1156:1165	elevated thermal resistance	1139:1165	elevated thermal resistance	1139:1165	Gp110 is a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance.
27287318	5	73	theme	specific	863:870	arg1	activity					872:879	a higher specific activity	854:879	a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity)	854:1054	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	5	73	theme	specific	863:870	arg1	U/μM					889:892	34,240 U/μM	882:892	34,240 U/μM	882:892	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	12	74	theme	enzymatic	2019:2027	arg1	activity					2029:2036	this high enzymatic activity	2009:2036	this high enzymatic activity	2009:2036	A domain of unknown function (DUF3380) is responsible for this high enzymatic activity.
27287318	5	75	theme	bacteriophage	711:723	arg1	Gp110					736:740	Gp110	736:740	Gp110	736:740	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	5	75	theme	bacteriophage	711:723	arg1	endolysin					725:733	a novel Salmonella bacteriophage endolysin	692:733	a novel Salmonella bacteriophage endolysin	692:733	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	15	76	theme	cleavage	2522:2529	arg1	specificity					2531:2541	the same peptidoglycan cleavage specificity	2499:2541	the same peptidoglycan cleavage specificity	2499:2541	Three-dimensional structure predictions for Gp110 suggest a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique.
27287318	15	77	theme	Three-dimensional	2359:2375	arg1	predictions					2387:2397	Three-dimensional structure predictions	2359:2397	Three-dimensional structure predictions for Gp110	2359:2407	Three-dimensional structure predictions for Gp110 suggest a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique.
27287318	8	78	theme	attractive	1546:1555	arg1	enzyme					1557:1562	an attractive enzyme	1543:1562	it an attractive enzyme for developing novel antimicrobial agents	1540:1604	Gp110 is active against directly cross-linked peptidoglycans with various peptide stem compositions, making it an attractive enzyme for developing novel antimicrobial agents.
27287318	5	79	theme	function	796:803	arg1	domain					778:783	an uncharacterized domain	759:783	an uncharacterized domain of unknown function	759:803	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	5	79	theme	function	796:803	arg1	function					796:803	unknown function	788:803	unknown function	788:803	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	15	80	theme	same	2503:2506	arg1	specificity					2531:2541	the same peptidoglycan cleavage specificity	2499:2541	the same peptidoglycan cleavage specificity	2499:2541	Three-dimensional structure predictions for Gp110 suggest a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique.
27287318	2	81	theme	food	282:285	arg1	conservation					287:298	food conservation	282:298	food conservation	282:298	Current research focuses on their potential applications in medicine, in food conservation, and as biotechnological tools.
27287318	7	82	theme	N-acetylmuramic	1377:1391	arg1	acid					1393:1396	N-acetylmuramic acid	1377:1396	N-acetylmuramic acid	1377:1396	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	11	83	theme	enzymatic	1828:1836	arg1	activity					1838:1845	The enzymatic activity	1824:1845	The enzymatic activity of this endolysin	1824:1863	The enzymatic activity of this endolysin exceeds that of all other endolysins previously characterized using the same methods.
27287318	1	84	theme	bacterial	178:186	arg1	layer					202:206	the bacterial peptidoglycan layer	174:206	the bacterial peptidoglycan layer	174:206	UNLABELLED Bacteriophage-encoded endolysins are highly diverse enzymes that cleave the bacterial peptidoglycan layer.
27287318	15	85	theme	unique	2572:2577	arg1	endolysin					2556:2564	this endolysin	2551:2564	this endolysin quite unique	2551:2577	Three-dimensional structure predictions for Gp110 suggest a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique.
27287318	3	86	theme	applications	354:365	arg1	wealth					344:349	the wealth	340:349	the wealth of applications relying on the use of endolysin	340:397	Despite the wealth of applications relying on the use of endolysin, little is known about the enzymatic properties of these enzymes, especially in the case of endolysins of bacteriophages infecting Gram-negative species.
27287318	8	87	theme	novel	1579:1583	arg1	agents					1599:1604	novel antimicrobial agents	1579:1604	novel antimicrobial agents	1579:1604	Gp110 is active against directly cross-linked peptidoglycans with various peptide stem compositions, making it an attractive enzyme for developing novel antimicrobial agents.
27287318	16	88	theme	endolysin-based	2704:2718	arg1	antibacterials					2720:2733	novel endolysin-based antibacterials	2698:2733	novel endolysin-based antibacterials	2698:2733	All of these features, combined with increased thermal resistance, make Gp110 an attractive candidate for engineering novel endolysin-based antibacterials.
27287318	5	89	theme	uncharacterized	762:776	arg1	domain					778:783	an uncharacterized domain	759:783	an uncharacterized domain of unknown function	759:803	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	5	89	theme	uncharacterized	762:776	arg1	function					796:803	unknown function	788:803	unknown function	788:803	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	7	90	contain	has	1289:1291	arg2	activity					1323:1330	N-acetylmuramidase (lysozyme) activity	1293:1330	N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues	1293:1429	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	7	90	contain	has	1289:1291	arg1	DUF3380					1281:1287	DUF3380	1281:1287	DUF3380	1281:1287	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	12	91	theme	high	2014:2017	arg1	activity					2029:2036	this high enzymatic activity	2009:2036	this high enzymatic activity	2009:2036	A domain of unknown function (DUF3380) is responsible for this high enzymatic activity.
27287318	3	92	theme	endolysin	389:397	arg1	use					382:384	the use	378:384	the use of endolysin	378:397	Despite the wealth of applications relying on the use of endolysin, little is known about the enzymatic properties of these enzymes, especially in the case of endolysins of bacteriophages infecting Gram-negative species.
27287318	6	93	theme	pH	1130:1131	arg1	activity					1118:1125	enzymatic activity	1108:1125	enzymatic activity of pH 8	1108:1133	Gp110 is a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance.
27287318	13	94	theme	stem	2160:2163	arg1	compositions					2165:2176	various peptide stem compositions	2144:2176	various peptide stem compositions	2144:2176	We report that DUF3380 has N-acetylmuramidase activity against directly cross-linked peptidoglycans with various peptide stem compositions.
27287318	2	95	from	applications	253:264	arg1	medicine					269:276	medicine	269:276	medicine	269:276	Current research focuses on their potential applications in medicine, in food conservation, and as biotechnological tools.
27287318	2	95	from	applications	253:264	arg1	conservation					287:298	food conservation	282:298	food conservation	282:298	Current research focuses on their potential applications in medicine, in food conservation, and as biotechnological tools.
27287318	7	96	theme	glycosidic	1353:1362	arg1	bond					1364:1367	the β-(1,4) glycosidic bond	1341:1367	the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues	1341:1429	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	11	97	theme	endolysin	1855:1863	arg1	activity					1838:1845	The enzymatic activity	1824:1845	The enzymatic activity of this endolysin	1824:1863	The enzymatic activity of this endolysin exceeds that of all other endolysins previously characterized using the same methods.
27287318	10	98	theme	modular	1738:1744	arg1	structure					1746:1754	a modular structure	1736:1754	a modular structure with an enzymatically active domain and a cell wall binding domain	1736:1821	This endolysin has a modular structure with an enzymatically active domain and a cell wall binding domain.
27287318	5	99	theme	C	833:833	arg1	terminus					835:842	its C terminus	829:842	its C terminus	829:842	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	1	100	theme	UNLABELLED	91:100	arg1	enzymes					154:160	highly diverse enzymes	139:160	highly diverse enzymes that cleave the bacterial peptidoglycan layer	139:206	UNLABELLED Bacteriophage-encoded endolysins are highly diverse enzymes that cleave the bacterial peptidoglycan layer.
27287318	1	100	theme	UNLABELLED	91:100	arg1	endolysins					124:133	UNLABELLED Bacteriophage-encoded endolysins	91:133	UNLABELLED Bacteriophage-encoded endolysins	91:133	UNLABELLED Bacteriophage-encoded endolysins are highly diverse enzymes that cleave the bacterial peptidoglycan layer.
27287318	8	101	theme	antimicrobial	1585:1597	arg1	agents					1599:1604	novel antimicrobial agents	1579:1604	novel antimicrobial agents	1579:1604	Gp110 is active against directly cross-linked peptidoglycans with various peptide stem compositions, making it an attractive enzyme for developing novel antimicrobial agents.
27287318	13	102	contain	has	2062:2064	arg1	DUF3380					2054:2060	DUF3380	2054:2060	DUF3380	2054:2060	We report that DUF3380 has N-acetylmuramidase activity against directly cross-linked peptidoglycans with various peptide stem compositions.
27287318	13	102	contain	has	2062:2064	arg2	activity					2085:2092	N-acetylmuramidase activity	2066:2092	N-acetylmuramidase activity against directly cross-linked peptidoglycans with various peptide stem compositions	2066:2176	We report that DUF3380 has N-acetylmuramidase activity against directly cross-linked peptidoglycans with various peptide stem compositions.
27287318	9	103	dep	IMPORTANCE	1607:1616	arg1	We					1618:1619	We	1618:1619	We	1618:1619	IMPORTANCE We report the functional and biochemical characterization of the Salmonella phage endolysin Gp110.
27287318	7	104	theme	high-performance	1182:1197	arg1	RP-HPLC					1222:1228	RP-HPLC	1222:1228	RP-HPLC	1222:1228	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	7	104	theme	high-performance	1182:1197	arg1	chromatography					1206:1219	Reverse-phase high-performance liquid chromatography	1168:1219	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry	1168:1267	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
27287318	15	105	theme	structure	2377:2385	arg1	predictions					2387:2397	Three-dimensional structure predictions	2359:2397	Three-dimensional structure predictions for Gp110	2359:2407	Three-dimensional structure predictions for Gp110 suggest a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique.
27287318	14	106	theme	verified	2199:2206	arg1	activity					2208:2215	This experimentally verified activity	2179:2215	This experimentally verified activity	2179:2215	This experimentally verified activity allows better classification and understanding of the enzymatic activities of endolysins, which mostly are inferred by sequence similarities.
27287318	0	107	theme	Phage	33:37	arg1	Endolysin					39:47	a Salmonella Phage Endolysin	20:47	a Salmonella Phage Endolysin	20:47	DUF3380 Domain from a Salmonella Phage Endolysin Shows Potent N-Acetylmuramidase Activity.
27287318	12	108	theme	function	1971:1978	arg1	responsible					1993:2003	responsible	1993:2003	responsible	1993:2003	A domain of unknown function (DUF3380) is responsible for this high enzymatic activity.
27287318	12	108	theme	function	1971:1978	arg1	domain					1953:1958	A domain	1951:1958	A domain of unknown function (DUF3380)	1951:1988	A domain of unknown function (DUF3380) is responsible for this high enzymatic activity.
27287318	12	108	theme	function	1971:1978	arg1	function					1971:1978	unknown function	1963:1978	unknown function	1963:1978	A domain of unknown function (DUF3380) is responsible for this high enzymatic activity.
27287318	4	109	theme	genome	563:568	arg1	annotations					570:580	Automated genome annotations	553:580	Automated genome annotations	553:580	Automated genome annotations therefore remain to be confirmed.
27287318	13	110	theme	cross-linked	2111:2122	arg1	peptidoglycans					2124:2137	directly cross-linked peptidoglycans	2102:2137	directly cross-linked peptidoglycans with various peptide stem compositions	2102:2176	We report that DUF3380 has N-acetylmuramidase activity against directly cross-linked peptidoglycans with various peptide stem compositions.
27287318	16	111	theme	attractive	2661:2670	arg1	candidate					2672:2680	an attractive candidate	2658:2680	an attractive candidate for engineering novel endolysin-based antibacterials	2658:2733	All of these features, combined with increased thermal resistance, make Gp110 an attractive candidate for engineering novel endolysin-based antibacterials.
27287318	9	112	theme	phage	1694:1698	arg1	Gp110					1710:1714	the Salmonella phage endolysin Gp110	1679:1714	the Salmonella phage endolysin Gp110	1679:1714	IMPORTANCE We report the functional and biochemical characterization of the Salmonella phage endolysin Gp110.
27287318	6	113	theme	elevated	1139:1146	arg1	resistance					1156:1165	elevated thermal resistance	1139:1165	elevated thermal resistance	1139:1165	Gp110 is a modular endolysin with an optimal pH of enzymatic activity of pH 8 and elevated thermal resistance.
27287318	2	114	theme	potential	243:251	arg1	applications					253:264	their potential applications	237:264	their potential applications in medicine, in food conservation, and as biotechnological tools	237:329	Current research focuses on their potential applications in medicine, in food conservation, and as biotechnological tools.
27287318	3	115	theme	bacteriophages	505:518	arg1	endolysins					491:500	endolysins	491:500	endolysins of bacteriophages infecting Gram-negative species	491:550	Despite the wealth of applications relying on the use of endolysin, little is known about the enzymatic properties of these enzymes, especially in the case of endolysins of bacteriophages infecting Gram-negative species.
27287318	9	116	theme	Gp110	1710:1714	arg1	characterization					1659:1674	the functional and biochemical characterization	1628:1674	the functional and biochemical characterization of the Salmonella phage endolysin Gp110	1628:1714	IMPORTANCE We report the functional and biochemical characterization of the Salmonella phage endolysin Gp110.
27287318	8	117	theme	cross-linked	1465:1476	arg1	peptidoglycans					1478:1491	directly cross-linked peptidoglycans	1456:1491	directly cross-linked peptidoglycans with various peptide stem compositions	1456:1530	Gp110 is active against directly cross-linked peptidoglycans with various peptide stem compositions, making it an attractive enzyme for developing novel antimicrobial agents.
27287318	13	118	theme	N-acetylmuramidase	2066:2083	arg1	activity					2085:2092	N-acetylmuramidase activity	2066:2092	N-acetylmuramidase activity against directly cross-linked peptidoglycans with various peptide stem compositions	2066:2176	We report that DUF3380 has N-acetylmuramidase activity against directly cross-linked peptidoglycans with various peptide stem compositions.
27287318	3	119	theme	endolysins	491:500	arg1	case					483:486	the case	479:486	the case of endolysins of bacteriophages infecting Gram-negative species	479:550	Despite the wealth of applications relying on the use of endolysin, little is known about the enzymatic properties of these enzymes, especially in the case of endolysins of bacteriophages infecting Gram-negative species.
27287318	16	120	theme	novel	2698:2702	arg1	antibacterials					2720:2733	novel endolysin-based antibacterials	2698:2733	novel endolysin-based antibacterials	2698:2733	All of these features, combined with increased thermal resistance, make Gp110 an attractive candidate for engineering novel endolysin-based antibacterials.
27287318	5	121	theme	364-fold	1030:1037	arg1	activity					1046:1053	1.7- to 364-fold higher activity	1022:1053	1.7- to 364-fold higher activity	1022:1053	Here, we report the biochemical analysis and cleavage site determination of a novel Salmonella bacteriophage endolysin, Gp110, which comprises an uncharacterized domain of unknown function (DUF3380; pfam11860) in its C terminus and shows a higher specific activity (34,240 U/μM) than that of 14 previously characterized endolysins active against peptidoglycan from Gram-negative bacteria (corresponding to 1.7- to 364-fold higher activity).
27287318	15	122	with	enzymes	2486:2492	arg1	specificity					2531:2541	the same peptidoglycan cleavage specificity	2499:2541	the same peptidoglycan cleavage specificity	2499:2541	Three-dimensional structure predictions for Gp110 suggest a fold that is completely different from that of known structures of enzymes with the same peptidoglycan cleavage specificity, making this endolysin quite unique.
27287318	7	123	theme	chromatography	1206:1219	arg1	analysis					1231:1238	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis	1168:1238	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry	1168:1267	Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis coupled to mass spectrometry showed that DUF3380 has N-acetylmuramidase (lysozyme) activity cleaving the β-(1,4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues.
28420224	8	0	theme	lower	1163:1167	arg1	levels					1189:1194	lower relative TNF-α mRNA levels	1163:1194	lower relative TNF-α mRNA levels	1163:1194	Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation.
28420224	4	1	theme	New	423:425	arg1	formation					432:440	New bone formation	423:440	New bone formation	423:440	New bone formation was evaluated by histomorphometric analysis.
28420224	2	2	theme	animal	311:316	arg1	experiments					318:328	the animal experiments	307:328	the animal experiments	307:328	Forty Sprague Dawley rats were used for the animal experiments.
28420224	9	3	contain	had	1335:1337	arg2	expression					1351:1360	lower TNF-α expression	1339:1360	lower TNF-α expression	1339:1360	IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation.
28420224	9	3	contain	had	1335:1337	arg2	expression					1387:1396	higher OPG and Runx2 expression	1366:1396	higher OPG and Runx2 expression	1366:1396	IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation.
28420224	9	3	contain	had	1335:1337	arg1	group					1329:1333	the AL/HA/SF group	1316:1333	the AL/HA/SF group	1316:1333	IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation.
28420224	7	4	theme	bone	1007:1010	arg1	formation					1012:1020	significantly higher new bone formation	982:1020	significantly higher new bone formation	982:1020	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	1	5	theme	bone	156:159	arg1	capability					174:183	the in vivo bone regeneration capability	144:183	the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites	144:264	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	5	6	theme	quantitative	641:652	arg1	reaction					693:700	quantitative reverse transcription polymerase chain reaction	641:700	quantitative reverse transcription polymerase chain reaction (qRT-PCR)	641:710	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	5	6	theme	quantitative	641:652	arg1	qRT-PCR					703:709	qRT-PCR	703:709	qRT-PCR	703:709	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	5	7	theme	-α	581:582	arg1	expression					584:593	tumor necrosis factor (TNF)-α expression	554:593	tumor necrosis factor (TNF)-α expression	554:593	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	8	8	theme	mRNA	1184:1187	arg1	levels					1189:1194	lower relative TNF-α mRNA levels	1163:1194	lower relative TNF-α mRNA levels	1163:1194	Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation.
28420224	8	9	theme	mRNA	1214:1217	arg1	levels					1219:1224	higher FGF-23 mRNA levels	1200:1224	higher FGF-23 mRNA levels	1200:1224	Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation.
28420224	1	10	theme	AL/HA/silk	231:240	arg1	SF					251:252	SF	251:252	SF	251:252	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	1	10	theme	AL/HA/silk	231:240	arg1	fibroin					242:248	AL/HA/silk fibroin	231:248	AL/HA/silk fibroin (SF)	231:253	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	5	11	theme	reverse	654:660	arg1	reaction					693:700	quantitative reverse transcription polymerase chain reaction	641:700	quantitative reverse transcription polymerase chain reaction (qRT-PCR)	641:710	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	5	11	theme	reverse	654:660	arg1	qRT-PCR					703:709	qRT-PCR	703:709	qRT-PCR	703:709	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	5	12	theme	expression	584:593	arg1	level					545:549	the level	541:549	the level of tumor necrosis factor (TNF)-α expression	541:593	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	7	13	theme	control	1035:1041	arg1	p					1050:1050	p = 0.044	1050:1058	p = 0.044	1050:1058	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	7	13	theme	control	1035:1041	arg1	group					1043:1047	the control group	1031:1047	the control group (p = 0.044)	1031:1059	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	8	14	theme	TNF-α	1178:1182	arg1	levels					1189:1194	lower relative TNF-α mRNA levels	1163:1194	lower relative TNF-α mRNA levels	1163:1194	Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation.
28420224	10	15	theme	graft	1544:1548	arg1	material					1550:1557	the residual graft material	1531:1557	the residual graft material	1531:1557	Additionally, no evidence of the inflammatory reaction or giant cell formation was observed around the residual graft material.
28420224	1	16	theme	fibroin	242:248	arg1	composites					255:264	alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites	188:264	alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites	188:264	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	5	17	theme	transcription	662:674	arg1	reaction					693:700	quantitative reverse transcription polymerase chain reaction	641:700	quantitative reverse transcription polymerase chain reaction (qRT-PCR)	641:710	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	5	17	theme	transcription	662:674	arg1	qRT-PCR					703:709	qRT-PCR	703:709	qRT-PCR	703:709	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	9	18	theme	AL/HA/SF	1320:1327	arg1	group					1329:1333	the AL/HA/SF group	1316:1333	the AL/HA/SF group	1316:1333	IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation.
28420224	5	19	theme	tumor	554:558	arg1	factor					569:574	tumor necrosis factor	554:574	tumor necrosis factor (TNF)-α expression	554:593	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	5	19	theme	tumor	554:558	arg1	TNF					577:579	TNF	577:579	TNF	577:579	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	9	20	theme	Runx2	1381:1385	arg1	expression					1387:1396	higher OPG and Runx2 expression	1366:1396	higher OPG and Runx2 expression	1366:1396	IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation.
28420224	1	21	theme	regeneration	161:172	arg1	capability					174:183	the in vivo bone regeneration capability	144:183	the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites	144:264	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	5	22	theme	polymerase	676:685	arg1	reaction					693:700	quantitative reverse transcription polymerase chain reaction	641:700	quantitative reverse transcription polymerase chain reaction (qRT-PCR)	641:710	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	5	22	theme	polymerase	676:685	arg1	qRT-PCR					703:709	qRT-PCR	703:709	qRT-PCR	703:709	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	5	23	theme	necrosis	560:567	arg1	factor					569:574	tumor necrosis factor	554:574	tumor necrosis factor (TNF)-α expression	554:593	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	5	23	theme	necrosis	560:567	arg1	TNF					577:579	TNF	577:579	TNF	577:579	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	0	24	theme	Fibroin-Alginate-Hydroxyapatite	5:35	arg1	Particles					47:55	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles	0:55	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.	0:104	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.
28420224	6	25	theme	factor	806:811	arg1	FGF					814:816	fibroblast growth factor (FGF)-23	788:820	fibroblast growth factor (FGF)-23	788:820	Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation.
28420224	5	26	theme	factor	569:574	arg1	expression					584:593	tumor necrosis factor (TNF)-α expression	554:593	tumor necrosis factor (TNF)-α expression	554:593	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	5	27	theme	chain	687:691	arg1	reaction					693:700	quantitative reverse transcription polymerase chain reaction	641:700	quantitative reverse transcription polymerase chain reaction (qRT-PCR)	641:710	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	5	27	theme	chain	687:691	arg1	qRT-PCR					703:709	qRT-PCR	703:709	qRT-PCR	703:709	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	2	28	theme	Dawley	281:286	arg1	rats					288:291	Forty Sprague Dawley rats	267:291	Forty Sprague Dawley rats	267:291	Forty Sprague Dawley rats were used for the animal experiments.
28420224	7	29	theme	=	1052:1052	arg1	p					1050:1050	p = 0.044	1050:1058	p = 0.044	1050:1058	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	7	29	theme	=	1052:1052	arg1	group					1043:1047	the control group	1031:1047	the control group (p = 0.044)	1031:1059	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	10	30	theme	reaction	1478:1485	arg1	evidence					1449:1456	no evidence	1446:1456	no evidence of the inflammatory reaction or giant cell formation	1446:1509	Additionally, no evidence of the inflammatory reaction or giant cell formation was observed around the residual graft material.
28420224	0	31	theme	Silk	0:3	arg1	Particles					47:55	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles	0:55	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.	0:104	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.
28420224	2	32	used	used	298:301	arg2	rats					288:291	Forty Sprague Dawley rats	267:291	Forty Sprague Dawley rats	267:291	Forty Sprague Dawley rats were used for the animal experiments.
28420224	7	33	dep	did	1027:1029	arg1	weeks					1098:1102	four weeks	1093:1102	four weeks post implantation	1093:1120	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	7	33	dep	did	1027:1029	arg1	than					1022:1025	than	1022:1025	than	1022:1025	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	1	34	theme	alginate	188:195	arg1	composites					255:264	alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites	188:264	alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites	188:264	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	3	35	theme	Central	331:337	arg1	bone					349:352	Central calvarial bone	331:352	Central calvarial bone (diameter: 8.0 mm) defects	331:379	Central calvarial bone (diameter: 8.0 mm) defects were grafted with AL, AL/HA, or AL/HA/SF.
28420224	3	35	theme	Central	331:337	arg1	mm					369:370	diameter: 8.0 mm	355:370	diameter: 8.0 mm	355:370	Central calvarial bone (diameter: 8.0 mm) defects were grafted with AL, AL/HA, or AL/HA/SF.
28420224	11	36	theme	bone	1638:1641	arg1	engineering					1650:1660	bone tissue engineering	1638:1660	bone tissue engineering	1638:1660	We concluded that the AL/HA/SF composite could be effective as a scaffold for bone tissue engineering.
28420224	5	37	theme	group	534:538	arg1	immunocompatibility					506:524	the immunocompatibility	502:524	the immunocompatibility of each group	502:538	To demonstrate the immunocompatibility of each group, the level of tumor necrosis factor (TNF)-α expression was studied by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) at eight weeks post implantation.
28420224	0	38	dep	In	97:98	arg1	Vivo					100:103	Vivo	100:103	Vivo	100:103	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.
28420224	0	39	theme	Composite	37:45	arg1	Particles					47:55	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles	0:55	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.	0:104	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.
28420224	6	40	theme	transcription	863:875	arg1	Runx2					885:889	Runx2	885:889	Runx2	885:889	Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation.
28420224	6	40	theme	transcription	863:875	arg1	factor					877:882	Runt-related transcription factor	850:882	Runt-related transcription factor (Runx2)	850:890	Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation.
28420224	7	41	theme	AL/HA/SF	960:967	arg1	group					969:973	The AL/HA/SF group	956:973	The AL/HA/SF group	956:973	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	6	42	theme	growth	799:804	arg1	FGF					814:816	fibroblast growth factor (FGF)-23	788:820	fibroblast growth factor (FGF)-23	788:820	Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation.
28420224	1	43	dep	in	148:149	arg1	vivo					151:154	vivo	151:154	vivo	151:154	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	9	44	theme	higher	1366:1371	arg1	expression					1387:1396	higher OPG and Runx2 expression	1366:1396	higher OPG and Runx2 expression	1366:1396	IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation.
28420224	9	45	theme	TNF-α	1345:1349	arg1	expression					1351:1360	lower TNF-α expression	1339:1360	lower TNF-α expression	1339:1360	IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation.
28420224	0	46	theme	Bone	60:63	arg1	Applications					84:95	Bone Tissue Engineering Applications	60:95	Bone Tissue Engineering Applications In Vivo	60:103	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.
28420224	1	47	theme	composites	255:264	arg1	capability					174:183	the in vivo bone regeneration capability	144:183	the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites	144:264	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	6	48	theme	fibroblast	788:797	arg1	FGF					814:816	fibroblast growth factor (FGF)-23	788:820	fibroblast growth factor (FGF)-23	788:820	Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation.
28420224	6	49	theme	Runt-related	850:861	arg1	Runx2					885:889	Runx2	885:889	Runx2	885:889	Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation.
28420224	6	49	theme	Runt-related	850:861	arg1	factor					877:882	Runt-related transcription factor	850:882	Runt-related transcription factor (Runx2)	850:890	Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation.
28420224	9	50	theme	IHC	1286:1288	arg1	results					1290:1296	IHC results	1286:1296	IHC results	1286:1296	IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation.
28420224	10	51	theme	formation	1501:1509	arg1	evidence					1449:1456	no evidence	1446:1456	no evidence of the inflammatory reaction or giant cell formation	1446:1509	Additionally, no evidence of the inflammatory reaction or giant cell formation was observed around the residual graft material.
28420224	10	52	theme	giant	1490:1494	arg1	formation					1501:1509	giant cell formation	1490:1509	giant cell formation	1490:1509	Additionally, no evidence of the inflammatory reaction or giant cell formation was observed around the residual graft material.
28420224	1	53	dep	alginate	188:195	arg1	AL					198:199	AL	198:199	AL	198:199	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	10	54	theme	cell	1496:1499	arg1	formation					1501:1509	giant cell formation	1490:1509	giant cell formation	1490:1509	Additionally, no evidence of the inflammatory reaction or giant cell formation was observed around the residual graft material.
28420224	0	55	theme	Engineering	72:82	arg1	Applications					84:95	Bone Tissue Engineering Applications	60:95	Bone Tissue Engineering Applications In Vivo	60:103	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.
28420224	10	56	theme	inflammatory	1465:1476	arg1	reaction					1478:1485	the inflammatory reaction	1461:1485	the inflammatory reaction	1461:1485	Additionally, no evidence of the inflammatory reaction or giant cell formation was observed around the residual graft material.
28420224	8	57	theme	FGF-23	1207:1212	arg1	levels					1219:1224	higher FGF-23 mRNA levels	1200:1224	higher FGF-23 mRNA levels	1200:1224	Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation.
28420224	7	58	theme	=	1081:1081	arg1	0.035					1083:1087	p = 0.035	1079:1087	p = 0.035	1079:1087	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	7	58	theme	=	1081:1081	arg1	group					1072:1076	the AL group	1065:1076	the AL group (p = 0.035)	1065:1088	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	8	59	dep	showed	1156:1161	arg1	did					1248:1250	did	1248:1250	showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation	1156:1283	Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation.
28420224	8	60	theme	higher	1200:1205	arg1	levels					1219:1224	higher FGF-23 mRNA levels	1200:1224	higher FGF-23 mRNA levels	1200:1224	Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation.
28420224	1	61	theme	study	122:126	arg1	aim					110:112	The aim	106:112	The aim of this study	106:126	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	0	62	theme	Tissue	65:70	arg1	Applications					84:95	Bone Tissue Engineering Applications	60:95	Bone Tissue Engineering Applications In Vivo	60:103	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.
28420224	11	63	theme	tissue	1643:1648	arg1	engineering					1650:1660	bone tissue engineering	1638:1660	bone tissue engineering	1638:1660	We concluded that the AL/HA/SF composite could be effective as a scaffold for bone tissue engineering.
28420224	4	64	theme	bone	427:430	arg1	formation					432:440	New bone formation	423:440	New bone formation	423:440	New bone formation was evaluated by histomorphometric analysis.
28420224	7	65	theme	higher	996:1001	arg1	formation					1012:1020	significantly higher new bone formation	982:1020	significantly higher new bone formation	982:1020	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	11	66	theme	AL/HA/SF	1582:1589	arg1	composite					1591:1599	the AL/HA/SF composite	1578:1599	the AL/HA/SF composite	1578:1599	We concluded that the AL/HA/SF composite could be effective as a scaffold for bone tissue engineering.
28420224	11	66	theme	AL/HA/SF	1582:1589	arg1	effective					1610:1618	effective	1610:1618	effective	1610:1618	We concluded that the AL/HA/SF composite could be effective as a scaffold for bone tissue engineering.
28420224	7	67	theme	p	1079:1079	arg1	0.035					1083:1087	p = 0.035	1079:1087	p = 0.035	1079:1087	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	7	67	theme	p	1079:1079	arg1	group					1072:1076	the AL group	1065:1076	the AL group (p = 0.035)	1065:1088	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	4	68	theme	histomorphometric	459:475	arg1	analysis					477:484	histomorphometric analysis	459:484	histomorphometric analysis	459:484	New bone formation was evaluated by histomorphometric analysis.
28420224	8	69	theme	AL/HA/SF	1141:1148	arg1	group					1150:1154	the AL/HA/SF group	1137:1154	the AL/HA/SF group	1137:1154	Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation.
28420224	1	70	theme	AL/hydroxyapatite	203:219	arg1	composites					255:264	alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites	188:264	alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites	188:264	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	6	71	theme	osteogenic	760:769	arg1	markers					771:777	osteogenic markers	760:777	osteogenic markers	760:777	Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation.
28420224	6	71	theme	osteogenic	760:769	arg1	factor					877:882	Runt-related transcription factor	850:882	Runt-related transcription factor (Runx2)	850:890	Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation.
28420224	6	71	theme	osteogenic	760:769	arg1	osteoprotegerin					823:837	osteoprotegerin	823:837	osteoprotegerin (OPG)	823:843	Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation.
28420224	6	71	theme	osteogenic	760:769	arg1	FGF					814:816	fibroblast growth factor (FGF)-23	788:820	fibroblast growth factor (FGF)-23	788:820	Additionally, osteogenic markers, such as fibroblast growth factor (FGF)-23, osteoprotegerin (OPG), and Runt-related transcription factor (Runx2) were evaluated by qPCR or IHC at eight weeks post implantation.
28420224	9	72	theme	OPG	1373:1375	arg1	expression					1387:1396	higher OPG and Runx2 expression	1366:1396	higher OPG and Runx2 expression	1366:1396	IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation.
28420224	7	73	theme	new	1003:1005	arg1	formation					1012:1020	significantly higher new bone formation	982:1020	significantly higher new bone formation	982:1020	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	9	74	theme	lower	1339:1343	arg1	expression					1351:1360	lower TNF-α expression	1339:1360	lower TNF-α expression	1339:1360	IHC results demonstrated that the AL/HA/SF group had lower TNF-α expression and higher OPG and Runx2 expression at eight weeks post implantation.
28420224	8	75	theme	relative	1169:1176	arg1	levels					1189:1194	lower relative TNF-α mRNA levels	1163:1194	lower relative TNF-α mRNA levels	1163:1194	Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation.
28420224	0	76	theme	In	97:98	arg1	Applications					84:95	Bone Tissue Engineering Applications	60:95	Bone Tissue Engineering Applications In Vivo	60:103	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.
28420224	10	77	theme	residual	1535:1542	arg1	material					1550:1557	the residual graft material	1531:1557	the residual graft material	1531:1557	Additionally, no evidence of the inflammatory reaction or giant cell formation was observed around the residual graft material.
28420224	8	78	theme	other	1235:1239	arg1	groups					1241:1246	the other groups	1231:1246	the other groups	1231:1246	Additionally, the AL/HA/SF group showed lower relative TNF-α mRNA levels and higher FGF-23 mRNA levels than the other groups did at eight weeks post implantation.
28420224	3	79	theme	bone	349:352	arg1	defects					373:379	Central calvarial bone (diameter: 8.0 mm) defects	331:379	Central calvarial bone (diameter: 8.0 mm) defects	331:379	Central calvarial bone (diameter: 8.0 mm) defects were grafted with AL, AL/HA, or AL/HA/SF.
28420224	3	80	theme	diameter	355:362	arg1	bone					349:352	Central calvarial bone	331:352	Central calvarial bone (diameter: 8.0 mm) defects	331:379	Central calvarial bone (diameter: 8.0 mm) defects were grafted with AL, AL/HA, or AL/HA/SF.
28420224	3	80	theme	diameter	355:362	arg1	mm					369:370	diameter: 8.0 mm	355:370	diameter: 8.0 mm	355:370	Central calvarial bone (diameter: 8.0 mm) defects were grafted with AL, AL/HA, or AL/HA/SF.
28420224	0	81	from	Particles	47:55	arg1	Applications					84:95	Bone Tissue Engineering Applications	60:95	Bone Tissue Engineering Applications In Vivo	60:103	Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.
28420224	3	82	theme	calvarial	339:347	arg1	bone					349:352	Central calvarial bone	331:352	Central calvarial bone (diameter: 8.0 mm) defects	331:379	Central calvarial bone (diameter: 8.0 mm) defects were grafted with AL, AL/HA, or AL/HA/SF.
28420224	3	82	theme	calvarial	339:347	arg1	mm					369:370	diameter: 8.0 mm	355:370	diameter: 8.0 mm	355:370	Central calvarial bone (diameter: 8.0 mm) defects were grafted with AL, AL/HA, or AL/HA/SF.
28420224	1	83	theme	in	148:149	arg1	capability					174:183	the in vivo bone regeneration capability	144:183	the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites	144:264	The aim of this study was to evaluate the in vivo bone regeneration capability of alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites.
28420224	7	84	theme	AL	1069:1070	arg1	0.035					1083:1087	p = 0.035	1079:1087	p = 0.035	1079:1087	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
28420224	7	84	theme	AL	1069:1070	arg1	group					1072:1076	the AL group	1065:1076	the AL group (p = 0.035)	1065:1088	The AL/HA/SF group showed significantly higher new bone formation than did the control group (p = 0.044) and the AL group (p = 0.035) at four weeks post implantation.
26340627	0	0	theme	Hydroxide-Indocyanine	85:105	arg1	Nanocomposites					113:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	3	1	theme	nanoparticle	679:690	arg1	surface					692:698	the LDH nanoparticle surface	671:698	the LDH nanoparticle surface	671:698	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	7	2	theme	future	1547:1552	arg1	diagnosis					1574:1582	future non-invasive cancer diagnosis	1547:1582	future non-invasive cancer diagnosis	1547:1582	This new class of organic-inorganic hybrid nanocomposites can potentially serve as a platform for future non-invasive cancer diagnosis and therapy.
26340627	7	3	theme	non-invasive	1554:1565	arg1	diagnosis					1574:1582	future non-invasive cancer diagnosis	1547:1582	future non-invasive cancer diagnosis	1547:1582	This new class of organic-inorganic hybrid nanocomposites can potentially serve as a platform for future non-invasive cancer diagnosis and therapy.
26340627	1	4	theme	double	267:272	arg1	hydroxide					274:282	layered double hydroxide	259:282	chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system	237:325	We designed a study for photodynamic therapy (PDT) using chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system.
26340627	1	4	theme	double	267:272	arg1	LDH					285:287	LDH	285:287	LDH	285:287	We designed a study for photodynamic therapy (PDT) using chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system.
26340627	0	5	theme	Double	78:83	arg1	Nanocomposites					113:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	6	6	theme	due	1358:1360	arg1	LDH-NH₂-ICG					1301:1311	uncoated LDH-NH₂-ICG	1292:1311	uncoated LDH-NH₂-ICG	1292:1311	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	6	7	theme	chitosan-coated	1330:1344	arg1	LDH-NH₂-ICG					1346:1356	chitosan-coated LDH-NH₂-ICG	1330:1356	chitosan-coated LDH-NH₂-ICG	1330:1356	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	1	8	theme	hydroxide	274:282	arg1	nanoparticles					290:302	chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles	237:302	chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system	237:325	We designed a study for photodynamic therapy (PDT) using chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system.
26340627	0	9	theme	Nanocomposites	113:126	arg1	Characterization					14:29	Characterization	14:29	Characterization	14:29	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	0	9	theme	Nanocomposites	113:126	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	4	10	from	stabilization	881:893	arg1	interlayer					902:911	the interlayer	898:911	the interlayer of LDH	898:918	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	4	11	dep	leaching	940:947	arg1	the					936:938	the	936:938	the	936:938	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	7	12	theme	new	1454:1456	arg1	class					1458:1462	This new class	1449:1462	This new class of organic-inorganic hybrid nanocomposites	1449:1505	This new class of organic-inorganic hybrid nanocomposites can potentially serve as a platform for future non-invasive cancer diagnosis and therapy.
26340627	7	12	theme	new	1454:1456	arg1	platform					1534:1541	a platform	1532:1541	a platform for future non-invasive cancer diagnosis and therapy	1532:1594	This new class of organic-inorganic hybrid nanocomposites can potentially serve as a platform for future non-invasive cancer diagnosis and therapy.
26340627	3	13	theme	efficient	537:545	arg1	coating					590:596	The efficient positively charged polymer (chitosan (CS)) coating	533:596	The efficient positively charged polymer (chitosan (CS)) coating	533:596	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	7	14	theme	nanocomposites	1492:1505	arg1	class					1458:1462	This new class	1449:1462	This new class of organic-inorganic hybrid nanocomposites	1449:1505	This new class of organic-inorganic hybrid nanocomposites can potentially serve as a platform for future non-invasive cancer diagnosis and therapy.
26340627	7	14	theme	nanocomposites	1492:1505	arg1	platform					1534:1541	a platform	1532:1541	a platform for future non-invasive cancer diagnosis and therapy	1532:1594	This new class of organic-inorganic hybrid nanocomposites can potentially serve as a platform for future non-invasive cancer diagnosis and therapy.
26340627	0	15	theme	Green	107:111	arg1	Nanocomposites					113:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	5	16	theme	significant	1110:1120	arg1	impact					1122:1127	a significant impact	1108:1127	a significant impact	1108:1127	The results indicated that the polymer coating and the number of polymer coats have a significant impact on the photo-toxicity of the nano-composites.
26340627	6	17	theme	photo	1253:1257	arg1	effect					1271:1276	enhanced photo therapeutic effect	1244:1276	enhanced photo therapeutic effect	1244:1276	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	4	18	theme	unique	737:742	arg1	hybridization					744:756	The unique hybridization	733:756	The unique hybridization of organic-inorganic nanocomposites	733:792	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	4	19	from	metabolization	953:966	arg1	conditions					1012:1021	the physiological conditions	994:1021	the physiological conditions	994:1021	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	0	20	theme	Potential	132:140	arg1	Applications					142:153	Potential Applications	132:153	Potential Applications in Photodynamic Therapy	132:177	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	6	21	theme	single	1317:1322	arg1	layer					1324:1328	single layer	1317:1328	single layer	1317:1328	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	4	22	theme	organic-inorganic	761:777	arg1	nanocomposites					779:792	organic-inorganic nanocomposites	761:792	organic-inorganic nanocomposites	761:792	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	2	23	theme	LDH	500:502	arg1	interlayers					504:514	amine modified LDH interlayers	485:514	amine modified LDH interlayers	485:514	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	2	24	dep	green	423:427	arg1	indocyanine					411:421	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green	328:427	indocyanine	411:421	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	6	25	theme	chitosan	1192:1199	arg1	nanoparticles					1220:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles	1175:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles	1175:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	2	26	theme	NIR	389:391	arg1	ICG					430:432	ICG	430:432	ICG	430:432	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	2	26	theme	NIR	389:391	arg1	green					423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green	328:427	green	423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	4	27	theme	physiological	998:1010	arg1	conditions					1012:1021	the physiological conditions	994:1021	the physiological conditions	994:1021	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	4	28	theme	photosensitizer	865:879	arg1	stabilization					881:893	the photosensitizer stabilization	861:893	the photosensitizer stabilization	861:893	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	2	29	theme	modified	491:498	arg1	interlayers					504:514	amine modified LDH interlayers	485:514	amine modified LDH interlayers	485:514	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	6	30	theme	layer	1186:1190	arg1	nanoparticles					1220:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles	1175:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles	1175:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	7	31	theme	cancer	1567:1572	arg1	diagnosis					1574:1582	future non-invasive cancer diagnosis	1547:1582	future non-invasive cancer diagnosis	1547:1582	This new class of organic-inorganic hybrid nanocomposites can potentially serve as a platform for future non-invasive cancer diagnosis and therapy.
26340627	1	32	theme	photodynamic	204:215	arg1	PDT					226:228	PDT	226:228	PDT	226:228	We designed a study for photodynamic therapy (PDT) using chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system.
26340627	1	32	theme	photodynamic	204:215	arg1	therapy					217:223	photodynamic therapy	204:223	photodynamic therapy (PDT)	204:229	We designed a study for photodynamic therapy (PDT) using chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system.
26340627	5	33	contain	have	1103:1106	arg1	number					1079:1084	the number	1075:1084	the number of polymer coats	1075:1101	The results indicated that the polymer coating and the number of polymer coats have a significant impact on the photo-toxicity of the nano-composites.
26340627	5	33	contain	have	1103:1106	arg2	impact					1122:1127	a significant impact	1108:1127	a significant impact	1108:1127	The results indicated that the polymer coating and the number of polymer coats have a significant impact on the photo-toxicity of the nano-composites.
26340627	5	33	contain	have	1103:1106	arg1	coating					1063:1069	the polymer coating	1051:1069	the polymer coating	1051:1069	The results indicated that the polymer coating and the number of polymer coats have a significant impact on the photo-toxicity of the nano-composites.
26340627	2	34	dep	Food	330:333	arg1	A					328:328	A	328:328	A	328:328	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	2	35	theme	near-infrared	374:386	arg1	ICG					430:432	ICG	430:432	ICG	430:432	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	2	35	theme	near-infrared	374:386	arg1	green					423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green	328:427	green	423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	6	36	theme	enhanced	1244:1251	arg1	effect					1271:1276	enhanced photo therapeutic effect	1244:1276	enhanced photo therapeutic effect	1244:1276	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	6	37	theme	thermal	1427:1433	arg1	degradations					1435:1446	thermal degradations	1427:1446	thermal degradations	1427:1446	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	3	38	theme	charged	558:564	arg1	polymer					566:572	positively charged polymer	547:572	The efficient positively charged polymer (chitosan (CS)) coating	533:596	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	6	39	theme	uncoated	1292:1299	arg1	LDH-NH₂-ICG					1301:1311	uncoated LDH-NH₂-ICG	1292:1311	uncoated LDH-NH₂-ICG	1292:1311	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	6	40	theme	double	1179:1184	arg1	nanoparticles					1220:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles	1175:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles	1175:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	6	41	theme	coated	1201:1206	arg1	nanoparticles					1220:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles	1175:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles	1175:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	5	42	theme	polymer	1089:1095	arg1	coats					1097:1101	polymer coats	1089:1101	polymer coats	1089:1101	The results indicated that the polymer coating and the number of polymer coats have a significant impact on the photo-toxicity of the nano-composites.
26340627	3	43	theme	polymer	566:572	arg1	coating					590:596	The efficient positively charged polymer (chitosan (CS)) coating	533:596	The efficient positively charged polymer (chitosan (CS)) coating	533:596	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	2	44	theme	photoactive	440:450	arg1	properties					452:461	photoactive properties	440:461	photoactive properties	440:461	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	0	45	theme	Near-Infrared	50:62	arg1	Nanocomposites					113:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	4	46	from	leaching	940:947	arg1	conditions					1012:1021	the physiological conditions	994:1021	the physiological conditions	994:1021	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	4	47	theme	successful	822:831	arg1	therapy					846:852	more effective and successful photodynamic therapy	803:852	more effective and successful photodynamic therapy	803:852	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	2	48	theme	dye	406:408	arg1	ICG					430:432	ICG	430:432	ICG	430:432	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	2	48	theme	dye	406:408	arg1	green					423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green	328:427	green	423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	0	49	theme	Chitosan-Coated	34:48	arg1	Nanocomposites					113:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	1	50	theme	delivery	311:318	arg1	system					320:325	the delivery system	307:325	the delivery system	307:325	We designed a study for photodynamic therapy (PDT) using chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system.
26340627	0	51	theme	Photodynamic	158:169	arg1	Therapy					171:177	Photodynamic Therapy	158:177	Photodynamic Therapy	158:177	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	7	52	theme	organic-inorganic	1467:1483	arg1	nanocomposites					1492:1505	organic-inorganic hybrid nanocomposites	1467:1505	organic-inorganic hybrid nanocomposites	1467:1505	This new class of organic-inorganic hybrid nanocomposites can potentially serve as a platform for future non-invasive cancer diagnosis and therapy.
26340627	2	53	theme	fluorescent	394:404	arg1	ICG					430:432	ICG	430:432	ICG	430:432	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	2	53	theme	fluorescent	394:404	arg1	green					423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green	328:427	green	423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	0	54	theme	NIR	65:67	arg1	Nanocomposites					113:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	4	55	theme	LDH	916:918	arg1	interlayer					902:911	the interlayer	898:911	the interlayer of LDH	898:918	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	3	56	theme	cross	618:622	arg1	linkage					624:630	the cross linkage	614:630	the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer	614:730	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	5	57	theme	polymer	1055:1061	arg1	coating					1063:1069	the polymer coating	1051:1069	the polymer coating	1051:1069	The results indicated that the polymer coating and the number of polymer coats have a significant impact on the photo-toxicity of the nano-composites.
26340627	2	58	with	green	423:427	arg1	properties					452:461	photoactive properties	440:461	photoactive properties	440:461	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	2	59	theme	Administration	344:357	arg1	ICG					430:432	ICG	430:432	ICG	430:432	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	2	59	theme	Administration	344:357	arg1	green					423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green	328:427	green	423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	5	60	theme	nano-composites	1158:1172	arg1	photo-toxicity					1136:1149	the photo-toxicity	1132:1149	the photo-toxicity of the nano-composites	1132:1172	The results indicated that the polymer coating and the number of polymer coats have a significant impact on the photo-toxicity of the nano-composites.
26340627	6	61	theme	LDH-NH₂-ICG	1208:1218	arg1	nanoparticles					1220:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles	1175:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles	1175:1232	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	4	62	theme	photodynamic	833:844	arg1	therapy					846:852	more effective and successful photodynamic therapy	803:852	more effective and successful photodynamic therapy	803:852	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	2	63	theme	Drug	339:342	arg1	Administration					344:357	Drug Administration	339:357	Drug Administration	339:357	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	4	64	theme	photosensitizer	975:989	arg1	metabolization					953:966	metabolization	953:966	metabolization	953:966	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	4	64	theme	photosensitizer	975:989	arg1	leaching					940:947	leaching	940:947	leaching	940:947	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	6	65	theme	enhanced	1369:1376	arg1	protection					1378:1387	the enhanced protection	1365:1387	the enhanced protection to photosensitizers against photo and thermal degradations	1365:1446	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	6	66	theme	therapeutic	1259:1269	arg1	effect					1271:1276	enhanced photo therapeutic effect	1244:1276	enhanced photo therapeutic effect	1244:1276	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	7	67	theme	hybrid	1485:1490	arg1	nanocomposites					1492:1505	organic-inorganic hybrid nanocomposites	1467:1505	organic-inorganic hybrid nanocomposites	1467:1505	This new class of organic-inorganic hybrid nanocomposites can potentially serve as a platform for future non-invasive cancer diagnosis and therapy.
26340627	1	68	theme	chitosan	237:244	arg1	nanoparticles					290:302	chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles	237:302	chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system	237:325	We designed a study for photodynamic therapy (PDT) using chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system.
26340627	6	69	dep	LDH-NH₂-ICG	1301:1311	arg1	LDH-NH₂-ICG					1346:1356	chitosan-coated LDH-NH₂-ICG	1330:1356	chitosan-coated LDH-NH₂-ICG	1330:1356	The double layer chitosan coated LDH-NH₂-ICG nanoparticles exhibited enhanced photo therapeutic effect compared with uncoated LDH-NH₂-ICG and single layer chitosan-coated LDH-NH₂-ICG due to the enhanced protection to photosensitizers against photo and thermal degradations.
26340627	3	70	theme	surface	638:644	arg1	groups					652:657	surface amine groups	638:657	surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde	638:718	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	3	70	theme	surface	638:644	arg1	spacer					725:730	a spacer	723:730	a spacer	723:730	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	4	71	theme	nanocomposites	779:792	arg1	hybridization					744:756	The unique hybridization	733:756	The unique hybridization of organic-inorganic nanocomposites	733:792	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	5	72	theme	coats	1097:1101	arg1	number					1079:1084	the number	1075:1084	the number of polymer coats	1075:1101	The results indicated that the polymer coating and the number of polymer coats have a significant impact on the photo-toxicity of the nano-composites.
26340627	5	72	theme	coats	1097:1101	arg1	coating					1063:1069	the polymer coating	1051:1069	the polymer coating	1051:1069	The results indicated that the polymer coating and the number of polymer coats have a significant impact on the photo-toxicity of the nano-composites.
26340627	2	73	theme	Food	330:333	arg1	ICG					430:432	ICG	430:432	ICG	430:432	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	2	73	theme	Food	330:333	arg1	green					423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green	328:427	green	423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	1	74	theme	coated	246:251	arg1	nanoparticles					290:302	chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles	237:302	chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system	237:325	We designed a study for photodynamic therapy (PDT) using chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system.
26340627	0	75	theme	Layered	70:76	arg1	Nanocomposites					113:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites	34:126	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	3	76	theme	amine	646:650	arg1	groups					652:657	surface amine groups	638:657	surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde	638:718	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	3	76	theme	amine	646:650	arg1	spacer					725:730	a spacer	723:730	a spacer	723:730	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	4	77	theme	effective	808:816	arg1	therapy					846:852	more effective and successful photodynamic therapy	803:852	more effective and successful photodynamic therapy	803:852	The unique hybridization of organic-inorganic nanocomposites rendered more effective and successful photodynamic therapy due to the photosensitizer stabilization in the interlayer of LDH, which prevents the leaching and metabolization of the photosensitizer in the physiological conditions.
26340627	2	78	theme	approved	365:372	arg1	ICG					430:432	ICG	430:432	ICG	430:432	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	2	78	theme	approved	365:372	arg1	green					423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green	328:427	green	423:427	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	1	79	theme	Mg-Al	253:257	arg1	nanoparticles					290:302	chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles	237:302	chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system	237:325	We designed a study for photodynamic therapy (PDT) using chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system.
26340627	2	80	theme	amine	485:489	arg1	interlayers					504:514	amine modified LDH interlayers	485:514	amine modified LDH interlayers	485:514	A Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye, indocyanine green (ICG) with photoactive properties was intercalated into amine modified LDH interlayers by ion-exchange.
26340627	3	81	theme	LDH	675:677	arg1	surface					692:698	the LDH nanoparticle surface	671:698	the LDH nanoparticle surface	671:698	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	0	82	from	Applications	142:153	arg1	Therapy					171:177	Photodynamic Therapy	158:177	Photodynamic Therapy	158:177	Synthesis and Characterization of Chitosan-Coated Near-Infrared (NIR) Layered Double Hydroxide-Indocyanine Green Nanocomposites for Potential Applications in Photodynamic Therapy.
26340627	3	83	dep	polymer	566:572	arg1	CS					585:586	CS	585:586	CS	585:586	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	3	83	dep	polymer	566:572	arg1	chitosan					575:582	chitosan	575:582	chitosan (CS)	575:587	The efficient positively charged polymer (chitosan (CS)) coating was achieved by the cross linkage using surface amine groups modified on the LDH nanoparticle surface with glutaraldehyde as a spacer.
26340627	1	84	theme	layered	259:265	arg1	hydroxide					274:282	layered double hydroxide	259:282	chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system	237:325	We designed a study for photodynamic therapy (PDT) using chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system.
26340627	1	84	theme	layered	259:265	arg1	LDH					285:287	LDH	285:287	LDH	285:287	We designed a study for photodynamic therapy (PDT) using chitosan coated Mg-Al layered double hydroxide (LDH) nanoparticles as the delivery system.
28709527	1	0	theme	ions	347:350	arg1	removal					327:333	the removal	323:333	the removal of arsenite ions from aqueous solutions	323:373	This study aimed to investigate the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions.
28709527	8	1	from	effect	1149:1154	arg1	removal					1193:1199	arsenite removal	1184:1199	arsenite removal	1184:1199	The central composite design under the Response Surface Methodology (RSM) was employed in order to investigate the effect of independent variables on arsenite removal and to determine the optimum condition.
28709527	12	2	theme	initial	1661:1667	arg1	0.68mgL-1					1693:1701	0.68mgL-1	1693:1701	0.68mgL-1	1693:1701	The predicted optimal conditions for the maximum removal efficiency were achieved with initial arsenite concentration, 0.68mgL-1; C-nZVI, 0.3 (gL-1); time, 31.25 (min) and pH, 5.2.
28709527	12	2	theme	initial	1661:1667	arg1	concentration					1678:1690	initial arsenite concentration	1661:1690	initial arsenite concentration	1661:1690	The predicted optimal conditions for the maximum removal efficiency were achieved with initial arsenite concentration, 0.68mgL-1; C-nZVI, 0.3 (gL-1); time, 31.25 (min) and pH, 5.2.
28709527	4	3	theme	iron	643:646	arg1	nanoparticles					648:660	non-stabilized iron nanoparticles	628:660	non-stabilized iron nanoparticles	628:660	The transmission electron microscope (TEM) images depicted bulkier dendrite flocs of non-stabilized iron nanoparticles.
28709527	4	4	theme	transmission	547:558	arg1	TEM					581:583	TEM	581:583	TEM	581:583	The transmission electron microscope (TEM) images depicted bulkier dendrite flocs of non-stabilized iron nanoparticles.
28709527	4	4	theme	transmission	547:558	arg1	microscope					569:578	transmission electron microscope	547:578	The transmission electron microscope (TEM) images	543:591	The transmission electron microscope (TEM) images depicted bulkier dendrite flocs of non-stabilized iron nanoparticles.
28709527	8	5	theme	Surface	1082:1088	arg1	RSM					1103:1105	RSM	1103:1105	RSM	1103:1105	The central composite design under the Response Surface Methodology (RSM) was employed in order to investigate the effect of independent variables on arsenite removal and to determine the optimum condition.
28709527	8	5	theme	Surface	1082:1088	arg1	Methodology					1090:1100	the Response Surface Methodology	1069:1100	the Response Surface Methodology (RSM)	1069:1106	The central composite design under the Response Surface Methodology (RSM) was employed in order to investigate the effect of independent variables on arsenite removal and to determine the optimum condition.
28709527	7	6	theme	C-nZVI	1016:1021	arg1	composite					1023:1031	the C-nZVI composite	1012:1031	the C-nZVI composite	1012:1031	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	6	7	dep	discrete	838:845	arg1	well-dispersed					848:861	well-dispersed	848:861	well-dispersed	848:861	The scanning electron microscopy (SEM) image showed that C-nZVI is approximately discrete, well-dispersed and an almost spherical shape.
28709527	1	8	theme	carboxymethyl	256:268	arg1	C-nZVI					311:316	C-nZVI	311:316	C-nZVI	311:316	This study aimed to investigate the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions.
28709527	1	8	theme	carboxymethyl	256:268	arg1	nanoparticles					296:308	carboxymethyl cellulose-stabilized iron nanoparticles	256:308	carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI)	256:317	This study aimed to investigate the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions.
28709527	1	9	theme	aqueous	357:363	arg1	solutions					365:373	aqueous solutions	357:373	aqueous solutions	357:373	This study aimed to investigate the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions.
28709527	6	10	theme	spherical	877:885	arg1	shape					887:891	an almost spherical shape	867:891	an almost spherical shape	867:891	The scanning electron microscopy (SEM) image showed that C-nZVI is approximately discrete, well-dispersed and an almost spherical shape.
28709527	10	11	dep	prediction	1407:1416	arg1	the					1403:1405	the	1403:1405	the	1403:1405	Therefore, this model is used for the prediction and optimization of arsenite removal from water.
28709527	7	12	theme	X-ray	946:950	arg1	diffraction					952:962	X-ray diffraction	946:962	X-ray diffraction (XRD)	946:968	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	7	12	theme	X-ray	946:950	arg1	XRD					965:967	XRD	965:967	XRD	965:967	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	1	13	theme	cellulose-stabilized	270:289	arg1	C-nZVI					311:316	C-nZVI	311:316	C-nZVI	311:316	This study aimed to investigate the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions.
28709527	1	13	theme	cellulose-stabilized	270:289	arg1	nanoparticles					296:308	carboxymethyl cellulose-stabilized iron nanoparticles	256:308	carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI)	256:317	This study aimed to investigate the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions.
28709527	0	14	theme	arsenite	106:113	arg1	remediation					91:101	the remediation	87:101	the remediation of arsenite from water	87:124	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	11	15	theme	removal	1479:1485	arg1	efficiency					1487:1496	The maximum removal efficiency	1467:1496	The maximum removal efficiency	1467:1496	The maximum removal efficiency was estimated to be 100% when all parameters are considered simultaneously.
28709527	11	15	theme	removal	1479:1485	arg1	%					1521:1521	100%	1518:1521	100%	1518:1521	The maximum removal efficiency was estimated to be 100% when all parameters are considered simultaneously.
28709527	12	16	theme	predicted	1578:1586	arg1	conditions					1596:1605	The predicted optimal conditions	1574:1605	The predicted optimal conditions for the maximum removal efficiency	1574:1640	The predicted optimal conditions for the maximum removal efficiency were achieved with initial arsenite concentration, 0.68mgL-1; C-nZVI, 0.3 (gL-1); time, 31.25 (min) and pH, 5.2.
28709527	5	17	theme	nanoscale	676:684	arg1	particles					686:694	nanoscale particles	676:694	nanoscale particles	676:694	It described nanoscale particles as not discrete resulting from the aggregation of particles.
28709527	1	18	theme	iron	291:294	arg1	C-nZVI					311:316	C-nZVI	311:316	C-nZVI	311:316	This study aimed to investigate the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions.
28709527	1	18	theme	iron	291:294	arg1	nanoparticles					296:308	carboxymethyl cellulose-stabilized iron nanoparticles	256:308	carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI)	256:317	This study aimed to investigate the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions.
28709527	8	19	theme	optimum	1222:1228	arg1	condition					1230:1238	the optimum condition	1218:1238	the optimum condition	1218:1238	The central composite design under the Response Surface Methodology (RSM) was employed in order to investigate the effect of independent variables on arsenite removal and to determine the optimum condition.
28709527	8	20	theme	central	1038:1044	arg1	design					1056:1061	The central composite design	1034:1061	The central composite design under the Response Surface Methodology (RSM)	1034:1106	The central composite design under the Response Surface Methodology (RSM) was employed in order to investigate the effect of independent variables on arsenite removal and to determine the optimum condition.
28709527	2	21	theme	iron	434:437	arg1	nanoparticles					439:451	carboxymethyl cellulose-stabilized iron nanoparticles	399:451	carboxymethyl cellulose-stabilized iron nanoparticles	399:451	Iron nanoparticles and carboxymethyl cellulose-stabilized iron nanoparticles were freshly synthesized.
28709527	4	22	theme	nanoparticles	648:660	arg1	flocs					619:623	bulkier dendrite flocs	602:623	bulkier dendrite flocs of non-stabilized iron nanoparticles	602:660	The transmission electron microscope (TEM) images depicted bulkier dendrite flocs of non-stabilized iron nanoparticles.
28709527	9	23	theme	second-order	1258:1269	arg1	model					1271:1275	The reduced full second-order model	1241:1275	The reduced full second-order model	1241:1275	The reduced full second-order model indicated a well-fitted model since the experimental values were in good agreement with it.
28709527	9	24	theme	full	1253:1256	arg1	model					1271:1275	The reduced full second-order model	1241:1275	The reduced full second-order model	1241:1275	The reduced full second-order model indicated a well-fitted model since the experimental values were in good agreement with it.
28709527	6	25	theme	microscopy	779:788	arg1	image					796:800	The scanning electron microscopy (SEM) image	757:800	The scanning electron microscopy (SEM) image	757:800	The scanning electron microscopy (SEM) image showed that C-nZVI is approximately discrete, well-dispersed and an almost spherical shape.
28709527	2	26	theme	cellulose-stabilized	413:432	arg1	nanoparticles					439:451	carboxymethyl cellulose-stabilized iron nanoparticles	399:451	carboxymethyl cellulose-stabilized iron nanoparticles	399:451	Iron nanoparticles and carboxymethyl cellulose-stabilized iron nanoparticles were freshly synthesized.
28709527	4	27	theme	dendrite	610:617	arg1	flocs					619:623	bulkier dendrite flocs	602:623	bulkier dendrite flocs of non-stabilized iron nanoparticles	602:660	The transmission electron microscope (TEM) images depicted bulkier dendrite flocs of non-stabilized iron nanoparticles.
28709527	6	28	theme	electron	770:777	arg1	microscopy					779:788	scanning electron microscopy	761:788	The scanning electron microscopy (SEM) image	757:800	The scanning electron microscopy (SEM) image showed that C-nZVI is approximately discrete, well-dispersed and an almost spherical shape.
28709527	6	28	theme	electron	770:777	arg1	SEM					791:793	SEM	791:793	SEM	791:793	The scanning electron microscopy (SEM) image showed that C-nZVI is approximately discrete, well-dispersed and an almost spherical shape.
28709527	2	29	theme	carboxymethyl	399:411	arg1	nanoparticles					439:451	carboxymethyl cellulose-stabilized iron nanoparticles	399:451	carboxymethyl cellulose-stabilized iron nanoparticles	399:451	Iron nanoparticles and carboxymethyl cellulose-stabilized iron nanoparticles were freshly synthesized.
28709527	1	30	theme	nanoparticles	296:308	arg1	feasibility					241:251	the feasibility	237:251	the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions	237:373	This study aimed to investigate the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions.
28709527	8	31	theme	Response	1073:1080	arg1	RSM					1103:1105	RSM	1103:1105	RSM	1103:1105	The central composite design under the Response Surface Methodology (RSM) was employed in order to investigate the effect of independent variables on arsenite removal and to determine the optimum condition.
28709527	8	31	theme	Response	1073:1080	arg1	Methodology					1090:1100	the Response Surface Methodology	1069:1100	the Response Surface Methodology (RSM)	1069:1106	The central composite design under the Response Surface Methodology (RSM) was employed in order to investigate the effect of independent variables on arsenite removal and to determine the optimum condition.
28709527	6	32	theme	scanning	761:768	arg1	microscopy					779:788	scanning electron microscopy	761:788	The scanning electron microscopy (SEM) image	757:800	The scanning electron microscopy (SEM) image showed that C-nZVI is approximately discrete, well-dispersed and an almost spherical shape.
28709527	6	32	theme	scanning	761:768	arg1	SEM					791:793	SEM	791:793	SEM	791:793	The scanning electron microscopy (SEM) image showed that C-nZVI is approximately discrete, well-dispersed and an almost spherical shape.
28709527	9	33	theme	well-fitted	1289:1299	arg1	model					1301:1305	a well-fitted model	1287:1305	a well-fitted model since the experimental values were in good agreement with it	1287:1366	The reduced full second-order model indicated a well-fitted model since the experimental values were in good agreement with it.
28709527	1	34	from	solutions	365:373	arg1	removal					327:333	the removal	323:333	the removal of arsenite ions from aqueous solutions	323:373	This study aimed to investigate the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions.
28709527	4	35	theme	bulkier	602:608	arg1	flocs					619:623	bulkier dendrite flocs	602:623	bulkier dendrite flocs of non-stabilized iron nanoparticles	602:660	The transmission electron microscope (TEM) images depicted bulkier dendrite flocs of non-stabilized iron nanoparticles.
28709527	0	36	theme	response	136:143	arg1	RSM					166:168	RSM	166:168	RSM	166:168	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	0	36	theme	response	136:143	arg1	methodology					153:163	response surface methodology	136:163	the response surface methodology (RSM) model	132:175	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	9	37	theme	reduced	1245:1251	arg1	model					1271:1275	The reduced full second-order model	1241:1275	The reduced full second-order model	1241:1275	The reduced full second-order model indicated a well-fitted model since the experimental values were in good agreement with it.
28709527	7	38	theme	x-ray	916:920	arg1	EDAX					936:939	EDAX	936:939	EDAX	936:939	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	7	38	theme	x-ray	916:920	arg1	spectroscopy					922:933	The energy dispersive x-ray spectroscopy	894:933	The energy dispersive x-ray spectroscopy (EDAX)	894:940	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	12	39	dep	0.68mgL-1	1693:1701	arg1	C-nZVI					1704:1709	C-nZVI	1704:1709	C-nZVI	1704:1709	The predicted optimal conditions for the maximum removal efficiency were achieved with initial arsenite concentration, 0.68mgL-1; C-nZVI, 0.3 (gL-1); time, 31.25 (min) and pH, 5.2.
28709527	12	39	dep	0.68mgL-1	1693:1701	arg1	time					1724:1727	time	1724:1727	time	1724:1727	The predicted optimal conditions for the maximum removal efficiency were achieved with initial arsenite concentration, 0.68mgL-1; C-nZVI, 0.3 (gL-1); time, 31.25 (min) and pH, 5.2.
28709527	10	40	theme	removal	1447:1453	arg1	optimization					1422:1433	optimization	1422:1433	optimization	1422:1433	Therefore, this model is used for the prediction and optimization of arsenite removal from water.
28709527	10	40	theme	removal	1447:1453	arg1	prediction					1407:1416	prediction	1407:1416	prediction	1407:1416	Therefore, this model is used for the prediction and optimization of arsenite removal from water.
28709527	4	41	theme	non-stabilized	628:641	arg1	nanoparticles					648:660	non-stabilized iron nanoparticles	628:660	non-stabilized iron nanoparticles	628:660	The transmission electron microscope (TEM) images depicted bulkier dendrite flocs of non-stabilized iron nanoparticles.
28709527	7	42	theme	dispersive	905:914	arg1	EDAX					936:939	EDAX	936:939	EDAX	936:939	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	7	42	theme	dispersive	905:914	arg1	spectroscopy					922:933	The energy dispersive x-ray spectroscopy	894:933	The energy dispersive x-ray spectroscopy (EDAX)	894:940	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	0	43	theme	cellulose-stabilized	43:62	arg1	nanoparticles					69:81	carboxymethyl cellulose-stabilized iron nanoparticles	29:81	carboxymethyl cellulose-stabilized iron nanoparticles	29:81	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	10	44	from	optimization	1422:1433	arg1	water					1460:1464	water	1460:1464	water	1460:1464	Therefore, this model is used for the prediction and optimization of arsenite removal from water.
28709527	0	45	theme	methodology	153:163	arg1	model					171:175	the response surface methodology (RSM) model	132:175	the response surface methodology (RSM) model	132:175	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	3	46	contain	had	509:511	arg1	nanomaterials					495:507	The synthesized nanomaterials	479:507	The synthesized nanomaterials	479:507	The synthesized nanomaterials had a size of 10nm approximately.
28709527	3	46	contain	had	509:511	arg2	size					515:518	a size	513:518	a size of 10nm approximately	513:540	The synthesized nanomaterials had a size of 10nm approximately.
28709527	7	47	theme	energy	898:903	arg1	EDAX					936:939	EDAX	936:939	EDAX	936:939	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	7	47	theme	energy	898:903	arg1	spectroscopy					922:933	The energy dispersive x-ray spectroscopy	894:933	The energy dispersive x-ray spectroscopy (EDAX)	894:940	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	0	48	theme	carboxymethyl	29:41	arg1	nanoparticles					69:81	carboxymethyl cellulose-stabilized iron nanoparticles	29:81	carboxymethyl cellulose-stabilized iron nanoparticles	29:81	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	8	49	theme	composite	1046:1054	arg1	design					1056:1061	The central composite design	1034:1061	The central composite design under the Response Surface Methodology (RSM)	1034:1106	The central composite design under the Response Surface Methodology (RSM) was employed in order to investigate the effect of independent variables on arsenite removal and to determine the optimum condition.
28709527	0	50	theme	surface	145:151	arg1	RSM					166:168	RSM	166:168	RSM	166:168	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	0	50	theme	surface	145:151	arg1	methodology					153:163	response surface methodology	136:163	the response surface methodology (RSM) model	132:175	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	7	51	attach	presence	993:1000	arg2	Fe0					1005:1007	Fe0	1005:1007	Fe0	1005:1007	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	7	51	attach	presence	993:1000	arg1	composite					1023:1031	the C-nZVI composite	1012:1031	the C-nZVI composite	1012:1031	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	7	52	dep	spectroscopy	922:933	arg1	spectrum					970:977	spectrum	970:977	spectrum	970:977	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	10	53	from	prediction	1407:1416	arg1	water					1460:1464	water	1460:1464	water	1460:1464	Therefore, this model is used for the prediction and optimization of arsenite removal from water.
28709527	0	54	theme	nanoparticles	69:81	arg1	capability					15:24	the capability	11:24	the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water	11:124	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	5	55	theme	particles	746:754	arg1	aggregation					731:741	the aggregation	727:741	the aggregation of particles	727:754	It described nanoscale particles as not discrete resulting from the aggregation of particles.
28709527	3	56	theme	synthesized	483:493	arg1	nanomaterials					495:507	The synthesized nanomaterials	479:507	The synthesized nanomaterials	479:507	The synthesized nanomaterials had a size of 10nm approximately.
28709527	0	57	theme	iron	64:67	arg1	nanoparticles					69:81	carboxymethyl cellulose-stabilized iron nanoparticles	29:81	carboxymethyl cellulose-stabilized iron nanoparticles	29:81	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	4	58	theme	electron	560:567	arg1	TEM					581:583	TEM	581:583	TEM	581:583	The transmission electron microscope (TEM) images depicted bulkier dendrite flocs of non-stabilized iron nanoparticles.
28709527	4	58	theme	electron	560:567	arg1	microscope					569:578	transmission electron microscope	547:578	The transmission electron microscope (TEM) images	543:591	The transmission electron microscope (TEM) images depicted bulkier dendrite flocs of non-stabilized iron nanoparticles.
28709527	9	59	theme	experimental	1317:1328	arg1	values					1330:1335	the experimental values	1313:1335	the experimental values	1313:1335	The reduced full second-order model indicated a well-fitted model since the experimental values were in good agreement with it.
28709527	9	60	theme	good	1345:1348	arg1	agreement					1350:1358	good agreement	1345:1358	good agreement with it	1345:1366	The reduced full second-order model indicated a well-fitted model since the experimental values were in good agreement with it.
28709527	7	61	from	presence	993:1000	arg1	composite					1023:1031	the C-nZVI composite	1012:1031	the C-nZVI composite	1012:1031	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	8	62	theme	variables	1171:1179	arg1	effect					1149:1154	the effect	1145:1154	the effect of independent variables on arsenite removal	1145:1199	The central composite design under the Response Surface Methodology (RSM) was employed in order to investigate the effect of independent variables on arsenite removal and to determine the optimum condition.
28709527	0	63	from	water	120:124	arg1	remediation					91:101	the remediation	87:101	the remediation of arsenite from water	87:124	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	10	64	theme	arsenite	1438:1445	arg1	removal					1447:1453	arsenite removal	1438:1453	arsenite removal from water	1438:1464	Therefore, this model is used for the prediction and optimization of arsenite removal from water.
28709527	3	65	theme	10nm	523:526	arg1	size					515:518	a size	513:518	a size of 10nm approximately	513:540	The synthesized nanomaterials had a size of 10nm approximately.
28709527	8	66	theme	independent	1159:1169	arg1	variables					1171:1179	independent variables	1159:1179	independent variables	1159:1179	The central composite design under the Response Surface Methodology (RSM) was employed in order to investigate the effect of independent variables on arsenite removal and to determine the optimum condition.
28709527	4	67	theme	microscope	569:578	arg1	images					586:591	The transmission electron microscope (TEM) images	543:591	The transmission electron microscope (TEM) images	543:591	The transmission electron microscope (TEM) images depicted bulkier dendrite flocs of non-stabilized iron nanoparticles.
28709527	11	68	theme	maximum	1471:1477	arg1	efficiency					1487:1496	The maximum removal efficiency	1467:1496	The maximum removal efficiency	1467:1496	The maximum removal efficiency was estimated to be 100% when all parameters are considered simultaneously.
28709527	11	68	theme	maximum	1471:1477	arg1	%					1521:1521	100%	1518:1521	100%	1518:1521	The maximum removal efficiency was estimated to be 100% when all parameters are considered simultaneously.
28709527	8	69	theme	arsenite	1184:1191	arg1	removal					1193:1199	arsenite removal	1184:1199	arsenite removal	1184:1199	The central composite design under the Response Surface Methodology (RSM) was employed in order to investigate the effect of independent variables on arsenite removal and to determine the optimum condition.
28709527	10	70	used	used	1394:1397	arg2	model					1385:1389	this model	1380:1389	this model	1380:1389	Therefore, this model is used for the prediction and optimization of arsenite removal from water.
28709527	12	71	theme	arsenite	1669:1676	arg1	0.68mgL-1					1693:1701	0.68mgL-1	1693:1701	0.68mgL-1	1693:1701	The predicted optimal conditions for the maximum removal efficiency were achieved with initial arsenite concentration, 0.68mgL-1; C-nZVI, 0.3 (gL-1); time, 31.25 (min) and pH, 5.2.
28709527	12	71	theme	arsenite	1669:1676	arg1	concentration					1678:1690	initial arsenite concentration	1661:1690	initial arsenite concentration	1661:1690	The predicted optimal conditions for the maximum removal efficiency were achieved with initial arsenite concentration, 0.68mgL-1; C-nZVI, 0.3 (gL-1); time, 31.25 (min) and pH, 5.2.
28709527	10	72	from	water	1460:1464	arg1	optimization					1422:1433	optimization	1422:1433	optimization	1422:1433	Therefore, this model is used for the prediction and optimization of arsenite removal from water.
28709527	10	72	from	water	1460:1464	arg1	removal					1447:1453	arsenite removal	1438:1453	arsenite removal from water	1438:1464	Therefore, this model is used for the prediction and optimization of arsenite removal from water.
28709527	10	72	from	water	1460:1464	arg1	prediction					1407:1416	prediction	1407:1416	prediction	1407:1416	Therefore, this model is used for the prediction and optimization of arsenite removal from water.
28709527	12	73	theme	optimal	1588:1594	arg1	conditions					1596:1605	The predicted optimal conditions	1574:1605	The predicted optimal conditions for the maximum removal efficiency	1574:1640	The predicted optimal conditions for the maximum removal efficiency were achieved with initial arsenite concentration, 0.68mgL-1; C-nZVI, 0.3 (gL-1); time, 31.25 (min) and pH, 5.2.
28709527	12	74	theme	removal	1623:1629	arg1	efficiency					1631:1640	the maximum removal efficiency	1611:1640	the maximum removal efficiency	1611:1640	The predicted optimal conditions for the maximum removal efficiency were achieved with initial arsenite concentration, 0.68mgL-1; C-nZVI, 0.3 (gL-1); time, 31.25 (min) and pH, 5.2.
28709527	7	75	theme	Fe0	1005:1007	arg1	presence					993:1000	the presence	989:1000	the presence of Fe0 in the C-nZVI composite	989:1031	The energy dispersive x-ray spectroscopy (EDAX) and X-ray diffraction (XRD) spectrum confirmed the presence of Fe0 in the C-nZVI composite.
28709527	1	76	theme	arsenite	338:345	arg1	ions					347:350	arsenite ions	338:350	arsenite ions	338:350	This study aimed to investigate the feasibility of carboxymethyl cellulose-stabilized iron nanoparticles (C-nZVI) for the removal of arsenite ions from aqueous solutions.
28709527	0	77	dep	Predicting	0:9	arg1	optimization					191:202	optimization	191:202	optimization	191:202	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	0	77	dep	Predicting	0:9	arg1	Modeling					178:185	Modeling	178:185	Modeling	178:185	Predicting the capability of carboxymethyl cellulose-stabilized iron nanoparticles for the remediation of arsenite from water using the response surface methodology (RSM) model: Modeling and optimization.
28709527	2	78	theme	Iron	376:379	arg1	nanoparticles					381:393	Iron nanoparticles	376:393	Iron nanoparticles	376:393	Iron nanoparticles and carboxymethyl cellulose-stabilized iron nanoparticles were freshly synthesized.
28709527	9	79	with	agreement	1350:1358	arg1	it					1365:1366	it	1365:1366	it	1365:1366	The reduced full second-order model indicated a well-fitted model since the experimental values were in good agreement with it.
28709527	12	80	theme	maximum	1615:1621	arg1	efficiency					1631:1640	the maximum removal efficiency	1611:1640	the maximum removal efficiency	1611:1640	The predicted optimal conditions for the maximum removal efficiency were achieved with initial arsenite concentration, 0.68mgL-1; C-nZVI, 0.3 (gL-1); time, 31.25 (min) and pH, 5.2.
26017173	4	0	theme	post-cryopreservation	490:510	arg1	recovery					512:519	high post-cryopreservation recovery	485:519	high post-cryopreservation recovery	485:519	OBJECTIVE This study was carried out to develop an efficient and practical cryopreservation protocol for sugarcane with high post-cryopreservation recovery.
26017173	9	1	theme	V	1566:1566	arg1	cryo-plate					1568:1577	V cryo-plate	1566:1577	V cryo-plate	1566:1577	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	9	1	theme	V	1566:1566	arg1	method					1609:1614	an efficient and practical method	1582:1614	an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks	1582:1672	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	5	2	from	absence	801:807	arg1	solution					706:713	loading solution	698:713	loading solution	698:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	1	3	theme	sucrose	147:153	arg1	content					155:161	its high sucrose content	138:161	its high sucrose content	138:161	BACKGROUND Sugarcane is a tropical crop of major importance primarily for its high sucrose content.
26017173	5	4	theme	loading	698:704	arg1	solution					706:713	loading solution	698:713	loading solution	698:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	6	5	theme	MS	987:988	arg1	medium					990:995	semi-solid MS medium	976:995	semi-solid MS medium with 0.5 M sucrose	976:1014	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	6	6	theme	mm	918:919	arg1	length					897:902	a length	895:902	a length	895:902	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	5	7	theme	sucrose	673:679	arg1	concentration					681:693	sucrose concentration	673:693	sucrose concentration in loading solution	673:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	8	from	conditions	745:754	arg1	solution					706:713	loading solution	698:713	loading solution	698:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	6	9	theme	semi-solid	976:985	arg1	medium					990:995	semi-solid MS medium	976:995	semi-solid MS medium with 0.5 M sucrose	976:1014	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	5	10	theme	light	739:743	arg1	conditions					745:754	light conditions	739:754	light conditions after liquid nitrogen exposure	739:785	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	11	theme	V	604:604	arg1	method					617:622	the V cryo-plate method	600:622	the V cryo-plate method	600:622	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	12	theme	preculture	634:643	arg1	medium					645:650	preculture medium	634:650	preculture medium	634:650	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	6	13	theme	maximum	1134:1140	arg1	recovery					1149:1156	maximum (100%) recovery	1134:1156	maximum (100%) recovery after cryopreservation	1134:1179	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	6	13	theme	maximum	1134:1140	arg1	%					1146:1146	100%	1143:1146	100%	1143:1146	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	5	14	from	time	725:728	arg1	solution					706:713	loading solution	698:713	loading solution	698:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	3	15	theme	genetic	346:352	arg1	diversity					354:362	its genetic diversity	342:362	its genetic diversity	342:362	Cryopreservation of sugarcane germplasm is an appropriate approach for conserving its genetic diversity.
26017173	1	16	theme	BACKGROUND	64:73	arg1	Sugarcane					75:83	BACKGROUND Sugarcane	64:83	BACKGROUND Sugarcane	64:83	BACKGROUND Sugarcane is a tropical crop of major importance primarily for its high sucrose content.
26017173	1	16	theme	BACKGROUND	64:73	arg1	crop					99:102	a tropical crop	88:102	a tropical crop of major importance primarily for its high sucrose content	88:161	BACKGROUND Sugarcane is a tropical crop of major importance primarily for its high sucrose content.
26017173	7	17	from	cultures	1250:1257	arg1	dark					1266:1269	the dark	1262:1269	the dark	1262:1269	It was also observed that removing the alginate gel and keeping the cultures in the dark for 7 days after cryopreservation significantly improved recovery.
26017173	9	18	from	cryopreservation	1620:1635	arg1	genebanks					1664:1672	genebanks	1664:1672	genebanks	1664:1672	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	8	19	dep	%	1521:1521	arg1	to					1523:1524	to	1523:1524	to	1523:1524	After optimizing the cryopreservation conditions using sugarcane variety Ni-1, 10 additional varieties were cryopreserved using the optimized protocol, with regrowth ranging from 56.7% to 100%.
26017173	6	20	theme	2.0	1054:1056	arg1	M					1058:1058	M	1058:1058	M	1058:1058	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	2	21	theme	abiotic	242:248	arg1	stresses					250:257	biotic and abiotic stresses	231:257	biotic and abiotic stresses	231:257	It is difficult to conserve it in the field or in vitro because of biotic and abiotic stresses.
26017173	4	22	with	protocol	457:464	arg1	recovery					512:519	high post-cryopreservation recovery	485:519	high post-cryopreservation recovery	485:519	OBJECTIVE This study was carried out to develop an efficient and practical cryopreservation protocol for sugarcane with high post-cryopreservation recovery.
26017173	5	23	theme	alginate	812:819	arg1	gel					821:823	alginate gel	812:823	alginate gel	812:823	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	4	24	theme	high	485:488	arg1	recovery					512:519	high post-cryopreservation recovery	485:519	high post-cryopreservation recovery	485:519	OBJECTIVE This study was carried out to develop an efficient and practical cryopreservation protocol for sugarcane with high post-cryopreservation recovery.
26017173	6	25	dep	2.0	914:916	arg1	to					911:912	to	911:912	to	911:912	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	8	26	theme	100	1526:1528	arg1	%					1521:1521	%	1521:1521	%	1521:1521	After optimizing the cryopreservation conditions using sugarcane variety Ni-1, 10 additional varieties were cryopreserved using the optimized protocol, with regrowth ranging from 56.7% to 100%.
26017173	9	27	dep	CONCLUSION	1532:1541	arg1	showed					1554:1559	showed	1554:1559	showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks	1554:1672	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	5	28	theme	gel	821:823	arg1	size					653:656	size	653:656	size of shoot tips	653:670	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	28	theme	gel	821:823	arg1	composition					845:855	recovery medium composition	829:855	recovery medium composition	829:855	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	28	theme	gel	821:823	arg1	presence					788:795	presence	788:795	presence	788:795	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	28	theme	gel	821:823	arg1	conditions					745:754	light conditions	739:754	light conditions after liquid nitrogen exposure	739:785	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	28	theme	gel	821:823	arg1	concentration					681:693	sucrose concentration	673:693	sucrose concentration in loading solution	673:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	28	theme	gel	821:823	arg1	absence					801:807	absence	801:807	absence	801:807	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	28	theme	gel	821:823	arg1	time					725:728	exposure time	716:728	exposure time to PVS2	716:736	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	8	29	theme	optimized	1470:1478	arg1	protocol					1480:1487	the optimized protocol	1466:1487	the optimized protocol	1466:1487	After optimizing the cryopreservation conditions using sugarcane variety Ni-1, 10 additional varieties were cryopreserved using the optimized protocol, with regrowth ranging from 56.7% to 100%.
26017173	5	30	theme	shoot	661:665	arg1	tips					667:670	shoot tips	661:670	shoot tips	661:670	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	31	theme	nitrogen	769:776	arg1	exposure					778:785	liquid nitrogen exposure	762:785	liquid nitrogen exposure	762:785	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	9	32	theme	tips	1656:1659	arg1	cryopreservation					1620:1635	cryopreservation	1620:1635	cryopreservation of sugarcane shoot tips in genebanks	1620:1672	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	6	33	dep	length	897:902	arg1	treated					1027:1033	treated	1027:1033	treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min	1027:1094	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	6	33	dep	length	897:902	arg1	exposed					1100:1106	exposed	1100:1106	exposed to PVS2 for 30 min	1100:1125	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	5	34	theme	tips	667:670	arg1	size					653:656	size	653:656	size of shoot tips	653:670	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	34	theme	tips	667:670	arg1	composition					845:855	recovery medium composition	829:855	recovery medium composition	829:855	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	34	theme	tips	667:670	arg1	presence					788:795	presence	788:795	presence	788:795	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	34	theme	tips	667:670	arg1	conditions					745:754	light conditions	739:754	light conditions after liquid nitrogen exposure	739:785	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	34	theme	tips	667:670	arg1	concentration					681:693	sucrose concentration	673:693	sucrose concentration in loading solution	673:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	34	theme	tips	667:670	arg1	absence					801:807	absence	801:807	absence	801:807	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	34	theme	tips	667:670	arg1	time					725:728	exposure time	716:728	exposure time to PVS2	716:736	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	35	theme	cryo-plate	606:615	arg1	method					617:622	the V cryo-plate method	600:622	the V cryo-plate method	600:622	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	1	36	theme	tropical	90:97	arg1	Sugarcane					75:83	BACKGROUND Sugarcane	64:83	BACKGROUND Sugarcane	64:83	BACKGROUND Sugarcane is a tropical crop of major importance primarily for its high sucrose content.
26017173	1	36	theme	tropical	90:97	arg1	crop					99:102	a tropical crop	88:102	a tropical crop of major importance primarily for its high sucrose content	88:161	BACKGROUND Sugarcane is a tropical crop of major importance primarily for its high sucrose content.
26017173	8	37	theme	cryopreservation	1359:1374	arg1	conditions					1376:1385	the cryopreservation conditions	1355:1385	the cryopreservation conditions using sugarcane variety Ni-1	1355:1414	After optimizing the cryopreservation conditions using sugarcane variety Ni-1, 10 additional varieties were cryopreserved using the optimized protocol, with regrowth ranging from 56.7% to 100%.
26017173	5	38	theme	recovery	829:836	arg1	composition					845:855	recovery medium composition	829:855	recovery medium composition	829:855	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	9	39	theme	practical	1599:1607	arg1	cryo-plate					1568:1577	V cryo-plate	1566:1577	V cryo-plate	1566:1577	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	9	39	theme	practical	1599:1607	arg1	method					1609:1614	an efficient and practical method	1582:1614	an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks	1582:1672	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	8	40	with	protocol	1480:1487	arg1	regrowth					1495:1502	regrowth	1495:1502	regrowth ranging from 56.7% to 100%	1495:1529	After optimizing the cryopreservation conditions using sugarcane variety Ni-1, 10 additional varieties were cryopreserved using the optimized protocol, with regrowth ranging from 56.7% to 100%.
26017173	0	41	theme	sugarcane	20:28	arg1	Cryopreservation					0:15	Cryopreservation	0:15	Cryopreservation of sugarcane	0:28	Cryopreservation of sugarcane using the V cryo-plate technique.
26017173	5	42	from	size	653:656	arg1	solution					706:713	loading solution	698:713	loading solution	698:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	43	from	composition	845:855	arg1	solution					706:713	loading solution	698:713	loading solution	698:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	4	44	theme	practical	430:438	arg1	protocol					457:464	an efficient and practical cryopreservation protocol	413:464	an efficient and practical cryopreservation protocol for sugarcane with high post-cryopreservation recovery	413:519	OBJECTIVE This study was carried out to develop an efficient and practical cryopreservation protocol for sugarcane with high post-cryopreservation recovery.
26017173	7	45	theme	alginate	1221:1228	arg1	gel					1230:1232	the alginate gel	1217:1232	the alginate gel	1217:1232	It was also observed that removing the alginate gel and keeping the cultures in the dark for 7 days after cryopreservation significantly improved recovery.
26017173	0	46	theme	V	40:40	arg1	technique					53:61	the V cryo-plate technique	36:61	the V cryo-plate technique	36:61	Cryopreservation of sugarcane using the V cryo-plate technique.
26017173	6	47	theme	MS	952:953	arg1	medium					955:960	semi-solid 1/2 MS medium	937:960	semi-solid 1/2 MS medium for 1 day	937:970	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	6	48	theme	M	1075:1075	arg1	sucrose					1077:1083	1.6 M sucrose	1071:1083	1.6 M sucrose	1071:1083	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	9	49	theme	shoot	1650:1654	arg1	tips					1656:1659	sugarcane shoot tips	1640:1659	sugarcane shoot tips in genebanks	1640:1672	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	5	50	from	concentration	681:693	arg1	solution					706:713	loading solution	698:713	loading solution	698:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	9	51	theme	sugarcane	1640:1648	arg1	tips					1656:1659	sugarcane shoot tips	1640:1659	sugarcane shoot tips in genebanks	1640:1672	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	8	52	theme	sugarcane	1393:1401	arg1	variety					1403:1409	sugarcane variety	1393:1409	sugarcane variety Ni-1	1393:1414	After optimizing the cryopreservation conditions using sugarcane variety Ni-1, 10 additional varieties were cryopreserved using the optimized protocol, with regrowth ranging from 56.7% to 100%.
26017173	6	53	with	medium	990:995	arg1	sucrose					1008:1014	0.5 M sucrose	1002:1014	0.5 M sucrose	1002:1014	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	5	54	theme	liquid	762:767	arg1	exposure					778:785	liquid nitrogen exposure	762:785	liquid nitrogen exposure	762:785	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	6	55	theme	semi-solid	937:946	arg1	medium					955:960	semi-solid 1/2 MS medium	937:960	semi-solid 1/2 MS medium for 1 day	937:970	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	3	56	theme	sugarcane	280:288	arg1	germplasm					290:298	sugarcane germplasm	280:298	sugarcane germplasm	280:298	Cryopreservation of sugarcane germplasm is an appropriate approach for conserving its genetic diversity.
26017173	4	57	theme	cryopreservation	440:455	arg1	protocol					457:464	an efficient and practical cryopreservation protocol	413:464	an efficient and practical cryopreservation protocol for sugarcane with high post-cryopreservation recovery	413:519	OBJECTIVE This study was carried out to develop an efficient and practical cryopreservation protocol for sugarcane with high post-cryopreservation recovery.
26017173	8	58	theme	additional	1420:1429	arg1	varieties					1431:1439	10 additional varieties	1417:1439	10 additional varieties	1417:1439	After optimizing the cryopreservation conditions using sugarcane variety Ni-1, 10 additional varieties were cryopreserved using the optimized protocol, with regrowth ranging from 56.7% to 100%.
26017173	5	59	dep	MATERIALS	522:530	arg1	Factors					544:550	Factors	544:550	Factors	544:550	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	1	60	theme	major	107:111	arg1	importance					113:122	major importance	107:122	major importance	107:122	BACKGROUND Sugarcane is a tropical crop of major importance primarily for its high sucrose content.
26017173	0	61	theme	cryo-plate	42:51	arg1	technique					53:61	the V cryo-plate technique	36:61	the V cryo-plate technique	36:61	Cryopreservation of sugarcane using the V cryo-plate technique.
26017173	3	62	theme	germplasm	290:298	arg1	approach					318:325	an appropriate approach	303:325	an appropriate approach for conserving its genetic diversity	303:362	Cryopreservation of sugarcane germplasm is an appropriate approach for conserving its genetic diversity.
26017173	3	62	theme	germplasm	290:298	arg1	Cryopreservation					260:275	Cryopreservation	260:275	Cryopreservation of sugarcane germplasm	260:298	Cryopreservation of sugarcane germplasm is an appropriate approach for conserving its genetic diversity.
26017173	6	63	contain	containing	1043:1052	arg2	sucrose					1077:1083	1.6 M sucrose	1071:1083	1.6 M sucrose	1071:1083	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	6	63	contain	containing	1043:1052	arg2	glycerol					1060:1067	2.0 M glycerol	1054:1067	2.0 M glycerol	1054:1067	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	6	63	contain	containing	1043:1052	arg1	LS					1040:1041	LS	1040:1041	LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min	1040:1094	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	5	64	attach	presence	788:795	arg2	tips					667:670	shoot tips	661:670	shoot tips	661:670	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	64	attach	presence	788:795	arg1	solution					706:713	loading solution	698:713	loading solution	698:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	5	64	attach	presence	788:795	arg2	gel					821:823	alginate gel	812:823	alginate gel	812:823	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	1	65	theme	importance	113:122	arg1	Sugarcane					75:83	BACKGROUND Sugarcane	64:83	BACKGROUND Sugarcane	64:83	BACKGROUND Sugarcane is a tropical crop of major importance primarily for its high sucrose content.
26017173	1	65	theme	importance	113:122	arg1	crop					99:102	a tropical crop	88:102	a tropical crop of major importance primarily for its high sucrose content	88:161	BACKGROUND Sugarcane is a tropical crop of major importance primarily for its high sucrose content.
26017173	5	66	from	presence	788:795	arg1	solution					706:713	loading solution	698:713	loading solution	698:713	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	2	67	theme	biotic	231:236	arg1	stresses					250:257	biotic and abiotic stresses	231:257	biotic and abiotic stresses	231:257	It is difficult to conserve it in the field or in vitro because of biotic and abiotic stresses.
26017173	6	68	theme	1.6	1071:1073	arg1	M					1075:1075	M	1075:1075	M	1075:1075	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	4	69	dep	OBJECTIVE	365:373	arg1	carried					390:396	carried	390:396	was carried out to develop an efficient and practical cryopreservation protocol for sugarcane with high post-cryopreservation recovery	386:519	OBJECTIVE This study was carried out to develop an efficient and practical cryopreservation protocol for sugarcane with high post-cryopreservation recovery.
26017173	6	70	theme	M	1006:1006	arg1	sucrose					1008:1014	0.5 M sucrose	1002:1014	0.5 M sucrose	1002:1014	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	5	71	theme	exposure	716:723	arg1	time					725:728	exposure time	716:728	exposure time to PVS2	716:736	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	4	72	theme	efficient	416:424	arg1	protocol					457:464	an efficient and practical cryopreservation protocol	413:464	an efficient and practical cryopreservation protocol for sugarcane with high post-cryopreservation recovery	413:519	OBJECTIVE This study was carried out to develop an efficient and practical cryopreservation protocol for sugarcane with high post-cryopreservation recovery.
26017173	6	73	with	medium	955:960	arg1	sucrose					1008:1014	0.5 M sucrose	1002:1014	0.5 M sucrose	1002:1014	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	6	74	theme	M	1058:1058	arg1	glycerol					1060:1067	2.0 M glycerol	1054:1067	2.0 M glycerol	1054:1067	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	6	75	theme	0.5	1002:1004	arg1	M					1006:1006	M	1006:1006	M	1006:1006	RESULTS Shoot tips with a length of 1.5 to 2.0 mm, precultured on semi-solid 1/2 MS medium for 1 day and semi-solid MS medium with 0.5 M sucrose for 1 day, treated with LS containing 2.0 M glycerol + 1.6 M sucrose for 30 min and exposed to PVS2 for 30 min showed maximum (100%) recovery after cryopreservation.
26017173	3	76	theme	appropriate	306:316	arg1	approach					318:325	an appropriate approach	303:325	an appropriate approach for conserving its genetic diversity	303:362	Cryopreservation of sugarcane germplasm is an appropriate approach for conserving its genetic diversity.
26017173	3	76	theme	appropriate	306:316	arg1	Cryopreservation					260:275	Cryopreservation	260:275	Cryopreservation of sugarcane germplasm	260:298	Cryopreservation of sugarcane germplasm is an appropriate approach for conserving its genetic diversity.
26017173	8	77	dep	variety	1403:1409	arg1	Ni-1					1411:1414	Ni-1	1411:1414	sugarcane variety Ni-1	1393:1414	After optimizing the cryopreservation conditions using sugarcane variety Ni-1, 10 additional varieties were cryopreserved using the optimized protocol, with regrowth ranging from 56.7% to 100%.
26017173	9	78	from	genebanks	1664:1672	arg1	cryopreservation					1620:1635	cryopreservation	1620:1635	cryopreservation of sugarcane shoot tips in genebanks	1620:1672	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	8	79	theme	56.7	1517:1520	arg1	%					1521:1521	%	1521:1521	%	1521:1521	After optimizing the cryopreservation conditions using sugarcane variety Ni-1, 10 additional varieties were cryopreserved using the optimized protocol, with regrowth ranging from 56.7% to 100%.
26017173	9	80	theme	efficient	1585:1593	arg1	cryo-plate					1568:1577	V cryo-plate	1566:1577	V cryo-plate	1566:1577	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	9	80	theme	efficient	1585:1593	arg1	method					1609:1614	an efficient and practical method	1582:1614	an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks	1582:1672	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26017173	5	81	theme	medium	838:843	arg1	composition					845:855	recovery medium composition	829:855	recovery medium composition	829:855	MATERIALS AND METHODS Factors affecting regrowth after cryopreservation using the V cryo-plate method including preculture medium, size of shoot tips, sucrose concentration in loading solution, exposure time to PVS2, light conditions after liquid nitrogen exposure, presence and absence of alginate gel and recovery medium composition were studied.
26017173	1	82	theme	high	142:145	arg1	content					155:161	its high sucrose content	138:161	its high sucrose content	138:161	BACKGROUND Sugarcane is a tropical crop of major importance primarily for its high sucrose content.
26017173	9	83	from	tips	1656:1659	arg1	genebanks					1664:1672	genebanks	1664:1672	genebanks	1664:1672	CONCLUSION This study showed that V cryo-plate is an efficient and practical method for cryopreservation of sugarcane shoot tips in genebanks.
26988183	0	0	theme	phototrophic	92:103	arg1	dinoflagellates					123:137	phototrophic and heterotrophic dinoflagellates	92:137	phototrophic and heterotrophic dinoflagellates	92:137	Differences in the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates.
26988183	6	1	dep	transform	968:976	arg1	infrared					978:985	infrared	978:985	transform infrared spectroscopy	968:998	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	2	2	theme	important	381:389	arg1	dinocyst					316:323	a preservable organic-walled resting cyst (dinocyst)	273:324	a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record	273:501	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	2	2	theme	important	381:389	arg1	link					391:394	an important link	378:394	an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record	378:501	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	10	3	theme	sedimentary	1936:1946	arg1	record					1948:1953	the sedimentary record	1932:1953	the sedimentary record	1932:1953	Our results indicated phylogeny was less important for predicting composition than the nutritional strategy of the dinoflagellate, suggesting potential for cyst wall chemistry to infer past nutritional strategies of extinct taxa preserved in the sedimentary record.
26988183	7	4	theme	different	1242:1250	arg1	compositions					1262:1273	two significantly different cyst wall compositions	1224:1273	two significantly different cyst wall compositions	1224:1273	Based on the presence of characteristic functional groups, two significantly different cyst wall compositions are observed that correspond to the dinoflagellate's nutritional strategy.
26988183	10	5	theme	extinct	1906:1912	arg1	taxa					1914:1917	extinct taxa	1906:1917	extinct taxa preserved in the sedimentary record	1906:1953	Our results indicated phylogeny was less important for predicting composition than the nutritional strategy of the dinoflagellate, suggesting potential for cyst wall chemistry to infer past nutritional strategies of extinct taxa preserved in the sedimentary record.
26988183	1	6	theme	planktonic	193:202	arg1	biomass					204:210	the planktonic biomass	189:210	the planktonic biomass from marine to freshwater environments	189:249	Dinoflagellates constitute a large proportion of the planktonic biomass from marine to freshwater environments.
26988183	10	7	theme	dinoflagellate	1805:1818	arg1	composition					1756:1766	composition	1756:1766	composition than the nutritional strategy of the dinoflagellate	1756:1818	Our results indicated phylogeny was less important for predicting composition than the nutritional strategy of the dinoflagellate, suggesting potential for cyst wall chemistry to infer past nutritional strategies of extinct taxa preserved in the sedimentary record.
26988183	0	8	theme	heterotrophic	109:121	arg1	dinoflagellates					123:137	phototrophic and heterotrophic dinoflagellates	92:137	phototrophic and heterotrophic dinoflagellates	92:137	Differences in the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates.
26988183	0	9	from	Differences	0:10	arg1	composition					28:38	the chemical composition	15:38	the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates	15:137	Differences in the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates.
26988183	5	10	theme	ecological	887:896	arg1	factors					899:905	inherent (phylogenetic) or variable (ecological) factors	850:905	factors	899:905	However, the extent to which this composition varies based on inherent (phylogenetic) or variable (ecological) factors has not been studied.
26988183	8	11	theme	cyst	1427:1430	arg1	wall					1432:1435	the cyst wall	1423:1435	the cyst wall produced by phototrophic dinoflagellates	1423:1476	The dinocyst wall compositions analyzed appeared carbohydrate-based, but the cyst wall produced by phototrophic dinoflagellates suggested a cellulose-like glucan, while heterotrophic forms produced a nitrogen-rich glycan.
26988183	9	12	dep	evidence	1609:1616	arg1	related					1642:1648	related	1642:1648	related	1642:1648	This constitutes the first empirical evidence nutritional strategy is related to different dinocyst wall chemistries.
26988183	1	13	from	proportion	175:184	arg1	marine					217:222	marine	217:222	marine	217:222	Dinoflagellates constitute a large proportion of the planktonic biomass from marine to freshwater environments.
26988183	4	14	theme	wall	772:775	arg1	chemistry					777:785	the cyst wall chemistry	763:785	the cyst wall chemistry	763:785	It is likely that numerous factors, including phylogeny and life strategy, determine the cyst wall chemistry.
26988183	1	15	theme	large	169:173	arg1	proportion					175:184	a large proportion	167:184	a large proportion of the planktonic biomass from marine to freshwater environments	167:249	Dinoflagellates constitute a large proportion of the planktonic biomass from marine to freshwater environments.
26988183	7	16	theme	functional	1205:1214	arg1	groups					1216:1221	characteristic functional groups	1190:1221	characteristic functional groups	1190:1221	Based on the presence of characteristic functional groups, two significantly different cyst wall compositions are observed that correspond to the dinoflagellate's nutritional strategy.
26988183	6	17	theme	extant	1103:1108	arg1	orders					1110:1115	the extant orders	1099:1115	the extant orders	1099:1115	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	1	18	theme	biomass	204:210	arg1	proportion					175:184	a large proportion	167:184	a large proportion of the planktonic biomass from marine to freshwater environments	167:249	Dinoflagellates constitute a large proportion of the planktonic biomass from marine to freshwater environments.
26988183	7	19	theme	groups	1216:1221	arg1	presence					1178:1185	the presence	1174:1185	the presence of characteristic functional groups	1174:1221	Based on the presence of characteristic functional groups, two significantly different cyst wall compositions are observed that correspond to the dinoflagellate's nutritional strategy.
26988183	6	20	dep	species	1021:1027	arg1	Gymnodiniales					1132:1144	Gymnodiniales	1132:1144	Gymnodiniales	1132:1144	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	6	20	dep	species	1021:1027	arg1	Peridiniales					1151:1162	Peridiniales	1151:1162	Peridiniales	1151:1162	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	6	20	dep	species	1021:1027	arg1	Gonyaulacales					1117:1129	Gonyaulacales	1117:1129	Gonyaulacales	1117:1129	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	6	20	dep	species	1021:1027	arg1	species					1021:1027	nine cyst species	1011:1027	nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales	1011:1162	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	9	21	theme	first	1593:1597	arg1	evidence					1609:1616	the first empirical evidence	1589:1616	the first empirical evidence nutritional strategy is related to different dinocyst wall chemistries	1589:1687	This constitutes the first empirical evidence nutritional strategy is related to different dinocyst wall chemistries.
26988183	3	22	theme	proxy	552:556	arg1	indicators					558:567	proxy indicators	552:567	proxy indicators for environmental conditions	552:596	Despite their abundance and widespread usage as proxy indicators for environmental conditions, there is a lack of knowledge regarding the dinocyst wall chemical composition.
26988183	10	23	theme	past	1875:1878	arg1	strategies					1892:1901	past nutritional strategies	1875:1901	past nutritional strategies of extinct taxa preserved in the sedimentary record	1875:1953	Our results indicated phylogeny was less important for predicting composition than the nutritional strategy of the dinoflagellate, suggesting potential for cyst wall chemistry to infer past nutritional strategies of extinct taxa preserved in the sedimentary record.
26988183	0	24	from	composition	28:38	arg1	dinoflagellates					123:137	phototrophic and heterotrophic dinoflagellates	92:137	phototrophic and heterotrophic dinoflagellates	92:137	Differences in the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates.
26988183	7	25	theme	cyst	1252:1255	arg1	compositions					1262:1273	two significantly different cyst wall compositions	1224:1273	two significantly different cyst wall compositions	1224:1273	Based on the presence of characteristic functional groups, two significantly different cyst wall compositions are observed that correspond to the dinoflagellate's nutritional strategy.
26988183	9	26	theme	different	1653:1661	arg1	chemistries					1677:1687	different dinocyst wall chemistries	1653:1687	different dinocyst wall chemistries	1653:1687	This constitutes the first empirical evidence nutritional strategy is related to different dinocyst wall chemistries.
26988183	6	27	theme	heterotrophic	1064:1076	arg1	dinoflagellates					1078:1092	either phototrophic or heterotrophic dinoflagellates	1041:1092	either phototrophic or heterotrophic dinoflagellates	1041:1092	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	7	28	theme	characteristic	1190:1203	arg1	groups					1216:1221	characteristic functional groups	1190:1221	characteristic functional groups	1190:1221	Based on the presence of characteristic functional groups, two significantly different cyst wall compositions are observed that correspond to the dinoflagellate's nutritional strategy.
26988183	4	29	theme	life	738:741	arg1	strategy					743:750	life strategy	738:750	life strategy	738:750	It is likely that numerous factors, including phylogeny and life strategy, determine the cyst wall chemistry.
26988183	9	30	theme	dinocyst	1663:1670	arg1	chemistries					1677:1687	different dinocyst wall chemistries	1653:1687	different dinocyst wall chemistries	1653:1687	This constitutes the first empirical evidence nutritional strategy is related to different dinocyst wall chemistries.
26988183	5	31	theme	variable	877:884	arg1	factors					899:905	inherent (phylogenetic) or variable (ecological) factors	850:905	factors	899:905	However, the extent to which this composition varies based on inherent (phylogenetic) or variable (ecological) factors has not been studied.
26988183	0	32	theme	chemical	19:26	arg1	composition					28:38	the chemical composition	15:38	the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates	15:137	Differences in the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates.
26988183	3	33	theme	knowledge	618:626	arg1	lack					610:613	a lack	608:613	a lack of knowledge regarding the dinocyst wall chemical composition	608:675	Despite their abundance and widespread usage as proxy indicators for environmental conditions, there is a lack of knowledge regarding the dinocyst wall chemical composition.
26988183	8	34	theme	heterotrophic	1519:1531	arg1	forms					1533:1537	heterotrophic forms	1519:1537	heterotrophic forms	1519:1537	The dinocyst wall compositions analyzed appeared carbohydrate-based, but the cyst wall produced by phototrophic dinoflagellates suggested a cellulose-like glucan, while heterotrophic forms produced a nitrogen-rich glycan.
26988183	10	35	theme	taxa	1914:1917	arg1	strategies					1892:1901	past nutritional strategies	1875:1901	past nutritional strategies of extinct taxa preserved in the sedimentary record	1875:1953	Our results indicated phylogeny was less important for predicting composition than the nutritional strategy of the dinoflagellate, suggesting potential for cyst wall chemistry to infer past nutritional strategies of extinct taxa preserved in the sedimentary record.
26988183	3	36	theme	environmental	573:585	arg1	conditions					587:596	environmental conditions	573:596	environmental conditions	573:596	Despite their abundance and widespread usage as proxy indicators for environmental conditions, there is a lack of knowledge regarding the dinocyst wall chemical composition.
26988183	1	37	theme	freshwater	227:236	arg1	environments					238:249	freshwater environments	227:249	freshwater environments	227:249	Dinoflagellates constitute a large proportion of the planktonic biomass from marine to freshwater environments.
26988183	2	38	theme	parent	450:455	arg1	theca					464:468	their parent motile theca	444:468	their parent motile theca	444:468	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	10	39	theme	wall	1851:1854	arg1	chemistry					1856:1864	cyst wall chemistry	1846:1864	cyst wall chemistry	1846:1864	Our results indicated phylogeny was less important for predicting composition than the nutritional strategy of the dinoflagellate, suggesting potential for cyst wall chemistry to infer past nutritional strategies of extinct taxa preserved in the sedimentary record.
26988183	2	40	theme	sexual	337:342	arg1	phase					344:348	the sexual phase	333:348	the sexual phase of their life cycle	333:368	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	9	41	theme	nutritional	1618:1628	arg1	strategy					1630:1637	nutritional strategy	1618:1637	nutritional strategy	1618:1637	This constitutes the first empirical evidence nutritional strategy is related to different dinocyst wall chemistries.
26988183	8	42	theme	dinocyst	1354:1361	arg1	compositions					1368:1379	The dinocyst wall compositions	1350:1379	The dinocyst wall compositions analyzed	1350:1388	The dinocyst wall compositions analyzed appeared carbohydrate-based, but the cyst wall produced by phototrophic dinoflagellates suggested a cellulose-like glucan, while heterotrophic forms produced a nitrogen-rich glycan.
26988183	2	43	theme	sedimentary	484:494	arg1	record					496:501	the sedimentary record	480:501	the sedimentary record	480:501	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	8	44	theme	wall	1363:1366	arg1	compositions					1368:1379	The dinocyst wall compositions	1350:1379	The dinocyst wall compositions analyzed	1350:1388	The dinocyst wall compositions analyzed appeared carbohydrate-based, but the cyst wall produced by phototrophic dinoflagellates suggested a cellulose-like glucan, while heterotrophic forms produced a nitrogen-rich glycan.
26988183	9	45	theme	wall	1672:1675	arg1	chemistries					1677:1687	different dinocyst wall chemistries	1653:1687	different dinocyst wall chemistries	1653:1687	This constitutes the first empirical evidence nutritional strategy is related to different dinocyst wall chemistries.
26988183	2	46	theme	preservable	275:285	arg1	dinocyst					316:323	a preservable organic-walled resting cyst (dinocyst)	273:324	a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record	273:501	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	2	46	theme	preservable	275:285	arg1	link					391:394	an important link	378:394	an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record	378:501	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	10	47	theme	nutritional	1777:1787	arg1	strategy					1789:1796	the nutritional strategy	1773:1796	the nutritional strategy	1773:1796	Our results indicated phylogeny was less important for predicting composition than the nutritional strategy of the dinoflagellate, suggesting potential for cyst wall chemistry to infer past nutritional strategies of extinct taxa preserved in the sedimentary record.
26988183	3	48	theme	dinocyst	642:649	arg1	composition					665:675	the dinocyst wall chemical composition	638:675	the dinocyst wall chemical composition	638:675	Despite their abundance and widespread usage as proxy indicators for environmental conditions, there is a lack of knowledge regarding the dinocyst wall chemical composition.
26988183	3	49	theme	wall	651:654	arg1	composition					665:675	the dinocyst wall chemical composition	638:675	the dinocyst wall chemical composition	638:675	Despite their abundance and widespread usage as proxy indicators for environmental conditions, there is a lack of knowledge regarding the dinocyst wall chemical composition.
26988183	2	50	theme	motile	457:462	arg1	theca					464:468	their parent motile theca	444:468	their parent motile theca	444:468	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	7	51	theme	nutritional	1328:1338	arg1	strategy					1340:1347	the dinoflagellate's nutritional strategy	1307:1347	the dinoflagellate's nutritional strategy	1307:1347	Based on the presence of characteristic functional groups, two significantly different cyst wall compositions are observed that correspond to the dinoflagellate's nutritional strategy.
26988183	0	52	theme	dinoflagellate	58:71	arg1	cysts					81:85	organic-walled dinoflagellate resting cysts	43:85	organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates	43:137	Differences in the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates.
26988183	10	53	theme	cyst	1846:1849	arg1	chemistry					1856:1864	cyst wall chemistry	1846:1864	cyst wall chemistry	1846:1864	Our results indicated phylogeny was less important for predicting composition than the nutritional strategy of the dinoflagellate, suggesting potential for cyst wall chemistry to infer past nutritional strategies of extinct taxa preserved in the sedimentary record.
26988183	3	54	theme	chemical	656:663	arg1	composition					665:675	the dinocyst wall chemical composition	638:675	the dinocyst wall chemical composition	638:675	Despite their abundance and widespread usage as proxy indicators for environmental conditions, there is a lack of knowledge regarding the dinocyst wall chemical composition.
26988183	0	55	theme	organic-walled	43:56	arg1	cysts					81:85	organic-walled dinoflagellate resting cysts	43:85	organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates	43:137	Differences in the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates.
26988183	8	56	theme	phototrophic	1449:1460	arg1	dinoflagellates					1462:1476	phototrophic dinoflagellates	1449:1476	phototrophic dinoflagellates	1449:1476	The dinocyst wall compositions analyzed appeared carbohydrate-based, but the cyst wall produced by phototrophic dinoflagellates suggested a cellulose-like glucan, while heterotrophic forms produced a nitrogen-rich glycan.
26988183	10	57	theme	nutritional	1880:1890	arg1	strategies					1892:1901	past nutritional strategies	1875:1901	past nutritional strategies of extinct taxa preserved in the sedimentary record	1875:1953	Our results indicated phylogeny was less important for predicting composition than the nutritional strategy of the dinoflagellate, suggesting potential for cyst wall chemistry to infer past nutritional strategies of extinct taxa preserved in the sedimentary record.
26988183	8	58	theme	cellulose-like	1490:1503	arg1	glucan					1505:1510	a cellulose-like glucan	1488:1510	a cellulose-like glucan	1488:1510	The dinocyst wall compositions analyzed appeared carbohydrate-based, but the cyst wall produced by phototrophic dinoflagellates suggested a cellulose-like glucan, while heterotrophic forms produced a nitrogen-rich glycan.
26988183	6	59	used	used	949:952	arg2	we					946:947	we	946:947	we	946:947	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	0	60	from	dinoflagellates	123:137	arg1	cysts					81:85	organic-walled dinoflagellate resting cysts	43:85	organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates	43:137	Differences in the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates.
26988183	0	60	from	dinoflagellates	123:137	arg1	composition					28:38	the chemical composition	15:38	the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates	15:137	Differences in the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates.
26988183	9	61	theme	empirical	1599:1607	arg1	evidence					1609:1616	the first empirical evidence	1589:1616	the first empirical evidence nutritional strategy is related to different dinocyst wall chemistries	1589:1687	This constitutes the first empirical evidence nutritional strategy is related to different dinocyst wall chemistries.
26988183	2	62	theme	cycle	364:368	arg1	phase					344:348	the sexual phase	333:348	the sexual phase of their life cycle	333:368	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	4	63	theme	cyst	767:770	arg1	chemistry					777:785	the cyst wall chemistry	763:785	the cyst wall chemistry	763:785	It is likely that numerous factors, including phylogeny and life strategy, determine the cyst wall chemistry.
26988183	6	64	dep	micro-Fourier	954:966	arg1	transform					968:976	transform	968:976	transform infrared spectroscopy	968:998	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	2	65	theme	cyst	310:313	arg1	dinocyst					316:323	a preservable organic-walled resting cyst (dinocyst)	273:324	a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record	273:501	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	2	65	theme	cyst	310:313	arg1	link					391:394	an important link	378:394	an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record	378:501	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	1	66	from	marine	217:222	arg1	biomass					204:210	the planktonic biomass	189:210	the planktonic biomass from marine to freshwater environments	189:249	Dinoflagellates constitute a large proportion of the planktonic biomass from marine to freshwater environments.
26988183	1	66	from	marine	217:222	arg1	proportion					175:184	a large proportion	167:184	a large proportion of the planktonic biomass from marine to freshwater environments	167:249	Dinoflagellates constitute a large proportion of the planktonic biomass from marine to freshwater environments.
26988183	2	67	theme	life	359:362	arg1	cycle					364:368	their life cycle	353:368	their life cycle	353:368	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	6	68	theme	cyst	1016:1019	arg1	Gymnodiniales					1132:1144	Gymnodiniales	1132:1144	Gymnodiniales	1132:1144	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	6	68	theme	cyst	1016:1019	arg1	Peridiniales					1151:1162	Peridiniales	1151:1162	Peridiniales	1151:1162	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	6	68	theme	cyst	1016:1019	arg1	Gonyaulacales					1117:1129	Gonyaulacales	1117:1129	Gonyaulacales	1117:1129	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	6	68	theme	cyst	1016:1019	arg1	species					1021:1027	nine cyst species	1011:1027	nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales	1011:1162	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	0	69	theme	cysts	81:85	arg1	composition					28:38	the chemical composition	15:38	the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates	15:137	Differences in the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates.
26988183	8	70	theme	nitrogen-rich	1550:1562	arg1	glycan					1564:1569	a nitrogen-rich glycan	1548:1569	a nitrogen-rich glycan	1548:1569	The dinocyst wall compositions analyzed appeared carbohydrate-based, but the cyst wall produced by phototrophic dinoflagellates suggested a cellulose-like glucan, while heterotrophic forms produced a nitrogen-rich glycan.
26988183	2	71	theme	resting	302:308	arg1	dinocyst					316:323	a preservable organic-walled resting cyst (dinocyst)	273:324	a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record	273:501	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	2	71	theme	resting	302:308	arg1	link					391:394	an important link	378:394	an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record	378:501	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	6	72	theme	phototrophic	1048:1059	arg1	dinoflagellates					1078:1092	either phototrophic or heterotrophic dinoflagellates	1041:1092	either phototrophic or heterotrophic dinoflagellates	1041:1092	To address this, we used micro-Fourier transform infrared spectroscopy to analyze nine cyst species produced by either phototrophic or heterotrophic dinoflagellates from the extant orders Gonyaulacales, Gymnodiniales, and Peridiniales.
26988183	3	73	theme	widespread	532:541	arg1	usage					543:547	widespread usage	532:547	widespread usage as proxy indicators for environmental conditions	532:596	Despite their abundance and widespread usage as proxy indicators for environmental conditions, there is a lack of knowledge regarding the dinocyst wall chemical composition.
26988183	7	74	theme	wall	1257:1260	arg1	compositions					1262:1273	two significantly different cyst wall compositions	1224:1273	two significantly different cyst wall compositions	1224:1273	Based on the presence of characteristic functional groups, two significantly different cyst wall compositions are observed that correspond to the dinoflagellate's nutritional strategy.
26988183	0	75	theme	resting	73:79	arg1	cysts					81:85	organic-walled dinoflagellate resting cysts	43:85	organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates	43:137	Differences in the chemical composition of organic-walled dinoflagellate resting cysts from phototrophic and heterotrophic dinoflagellates.
26988183	2	76	theme	organic-walled	287:300	arg1	dinocyst					316:323	a preservable organic-walled resting cyst (dinocyst)	273:324	a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record	273:501	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	2	76	theme	organic-walled	287:300	arg1	link					391:394	an important link	378:394	an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record	378:501	Some species produce a preservable organic-walled resting cyst (dinocyst) during the sexual phase of their life cycle that is an important link between the organisms, the environment in which their parent motile theca grew, and the sedimentary record.
26988183	4	77	theme	numerous	696:703	arg1	factors					705:711	numerous factors	696:711	numerous factors	696:711	It is likely that numerous factors, including phylogeny and life strategy, determine the cyst wall chemistry.
26988183	4	77	theme	numerous	696:703	arg1	phylogeny					724:732	phylogeny	724:732	phylogeny	724:732	It is likely that numerous factors, including phylogeny and life strategy, determine the cyst wall chemistry.
26988183	4	77	theme	numerous	696:703	arg1	strategy					743:750	life strategy	738:750	life strategy	738:750	It is likely that numerous factors, including phylogeny and life strategy, determine the cyst wall chemistry.
25864234	5	0	theme	mean	727:730	arg1	thickness					732:740	mean thickness	727:740	mean thickness	727:740	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	2	1	theme	different	124:132	arg1	materials					146:154	different restorative materials	124:154	different restorative materials	124:154	PURPOSE To evaluate the influence of different restorative materials on the biofilm structure accumulated in situ.
25864234	3	2	theme	material	227:234	arg1	discs					213:217	METHODS 15 discs	202:217	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam)	202:318	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	6	3	theme	Kolmogorov-Smirnov	856:873	arg1	tests					894:898	the Kolmogorov-Smirnov and Kruskal-Wallis tests	852:898	the Kolmogorov-Smirnov and Kruskal-Wallis tests (P ≤ 5%)	852:907	The statistical analysis was performed by using the Kolmogorov-Smirnov and Kruskal-Wallis tests (P ≤ 5%).
25864234	5	4	theme	descriptive	606:616	arg1	analysis					618:625	descriptive analysis	606:625	descriptive analysis about cell viability and architecture	606:663	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	6	5	theme	Kruskal-Wallis	879:892	arg1	tests					894:898	the Kolmogorov-Smirnov and Kruskal-Wallis tests	852:898	the Kolmogorov-Smirnov and Kruskal-Wallis tests (P ≤ 5%)	852:907	The statistical analysis was performed by using the Kolmogorov-Smirnov and Kruskal-Wallis tests (P ≤ 5%).
25864234	4	6	theme	scanning	538:545	arg1	CLSM					559:562	CLSM	559:562	CLSM	559:562	After 7 days, the specimens were carefully removed and visualized by confocal laser scanning microscopy (CLSM).
25864234	4	6	theme	scanning	538:545	arg1	microscopy					547:556	confocal laser scanning microscopy	523:556	confocal laser scanning microscopy (CLSM)	523:563	After 7 days, the specimens were carefully removed and visualized by confocal laser scanning microscopy (CLSM).
25864234	8	7	theme	cells	1130:1134	arg1	prevalence					1105:1114	visually a prevalence	1094:1114	visually a prevalence of non-viable cells forming small clusters distributed by the biofilm	1094:1184	However, qualitatively, glass-ionomer cements and amalgam showed visually a prevalence of non-viable cells forming small clusters distributed by the biofilm, and voids were presented in smaller proportion in the biofilm volume compared to composite and ceramic.
25864234	2	8	theme	biofilm	163:169	arg1	structure					171:179	the biofilm structure	159:179	the biofilm structure accumulated in situ	159:199	PURPOSE To evaluate the influence of different restorative materials on the biofilm structure accumulated in situ.
25864234	1	9	theme	CLSM/COMSTAT	64:75	arg1	analysis					77:84	CLSM/COMSTAT analysis	64:84	CLSM/COMSTAT analysis.	64:85	CLSM/COMSTAT analysis.
25864234	6	10	dep	tests	894:898	arg1	≤					903:903	≤	903:903	≤	903:903	The statistical analysis was performed by using the Kolmogorov-Smirnov and Kruskal-Wallis tests (P ≤ 5%).
25864234	3	11	theme	resin	246:250	arg1	composite					252:260	resin composite	246:260	ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam	237:317	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	5	12	dep	qualitatively	591:603	arg1	analysis					618:625	descriptive analysis	606:625	descriptive analysis about cell viability and architecture	606:663	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	5	13	theme	maximum	743:749	arg1	thickness					751:759	maximum thickness	743:759	maximum thickness	743:759	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	8	14	theme	small	1144:1148	arg1	clusters					1150:1157	small clusters	1144:1157	small clusters distributed by the biofilm	1144:1184	However, qualitatively, glass-ionomer cements and amalgam showed visually a prevalence of non-viable cells forming small clusters distributed by the biofilm, and voids were presented in smaller proportion in the biofilm volume compared to composite and ceramic.
25864234	3	15	dep	ceramic	237:243	arg1	amalgam					311:317	amalgam	311:317	ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam	237:317	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	3	15	dep	ceramic	237:243	arg1	glass-ionomers					295:308	resin-modified and conventional glass-ionomers	263:308	ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam	237:317	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	3	15	dep	ceramic	237:243	arg1	composite					252:260	resin composite	246:260	ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam	237:317	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	0	16	theme	In	0:1	arg1	accumulation					18:29	In situ bacterial accumulation	0:29	In situ bacterial accumulation on dental restorative materials.	0:62	In situ bacterial accumulation on dental restorative materials.
25864234	3	17	theme	%	424:424	arg1	solution					434:441	20% sucrose solution	422:441	20% sucrose solution	422:441	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	3	17	theme	%	424:424	arg1	challenge					411:419	a cariogenic challenge	398:419	a cariogenic challenge (20% sucrose solution, 10x/day)	398:451	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	5	18	theme	roughness	765:773	arg1	coefficient					775:785	roughness coefficient	765:785	roughness coefficient	765:785	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	2	19	theme	materials	146:154	arg1	influence					111:119	the influence	107:119	the influence of different restorative materials on the biofilm structure accumulated in situ	107:199	PURPOSE To evaluate the influence of different restorative materials on the biofilm structure accumulated in situ.
25864234	7	20	theme	different	1008:1016	arg1	materials					1018:1026	different materials	1008:1026	different materials	1008:1026	RESULTS The medians of the biofilm parameters analyzed showed no statistical difference regarding different materials.
25864234	2	21	from	influence	111:119	arg1	structure					171:179	the biofilm structure	159:179	the biofilm structure accumulated in situ	159:199	PURPOSE To evaluate the influence of different restorative materials on the biofilm structure accumulated in situ.
25864234	8	22	theme	non-viable	1119:1128	arg1	cells					1130:1134	non-viable cells	1119:1134	non-viable cells forming small clusters distributed by the biofilm	1119:1184	However, qualitatively, glass-ionomer cements and amalgam showed visually a prevalence of non-viable cells forming small clusters distributed by the biofilm, and voids were presented in smaller proportion in the biofilm volume compared to composite and ceramic.
25864234	8	23	theme	smaller	1215:1221	arg1	proportion					1223:1232	smaller proportion	1215:1232	smaller proportion in the biofilm volume	1215:1254	However, qualitatively, glass-ionomer cements and amalgam showed visually a prevalence of non-viable cells forming small clusters distributed by the biofilm, and voids were presented in smaller proportion in the biofilm volume compared to composite and ceramic.
25864234	3	24	dep	material	227:234	arg1	ceramic					237:243	ceramic	237:243	ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam	237:317	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	3	25	theme	METHODS	202:208	arg1	discs					213:217	METHODS 15 discs	202:217	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam)	202:318	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	5	26	theme	biofilm	794:800	arg1	area					709:712	area	709:712	area	709:712	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	5	26	theme	biofilm	794:800	arg1	thickness					751:759	maximum thickness	743:759	maximum thickness	743:759	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	5	26	theme	biofilm	794:800	arg1	coefficient					775:785	roughness coefficient	765:785	roughness coefficient	765:785	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	5	26	theme	biofilm	794:800	arg1	bio-volume					715:724	bio-volume	715:724	bio-volume	715:724	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	5	26	theme	biofilm	794:800	arg1	thickness					732:740	mean thickness	727:740	mean thickness	727:740	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	0	27	theme	bacterial	8:16	arg1	accumulation					18:29	In situ bacterial accumulation	0:29	In situ bacterial accumulation on dental restorative materials.	0:62	In situ bacterial accumulation on dental restorative materials.
25864234	4	28	theme	laser	532:536	arg1	CLSM					559:562	CLSM	559:562	CLSM	559:562	After 7 days, the specimens were carefully removed and visualized by confocal laser scanning microscopy (CLSM).
25864234	4	28	theme	laser	532:536	arg1	microscopy					547:556	confocal laser scanning microscopy	523:556	confocal laser scanning microscopy (CLSM)	523:563	After 7 days, the specimens were carefully removed and visualized by confocal laser scanning microscopy (CLSM).
25864234	3	29	theme	conventional	282:293	arg1	glass-ionomers					295:308	resin-modified and conventional glass-ionomers	263:308	ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam	237:317	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	0	30	theme	dental	34:39	arg1	materials					53:61	dental restorative materials	34:61	dental restorative materials	34:61	In situ bacterial accumulation on dental restorative materials.
25864234	4	31	theme	confocal	523:530	arg1	CLSM					559:562	CLSM	559:562	CLSM	559:562	After 7 days, the specimens were carefully removed and visualized by confocal laser scanning microscopy (CLSM).
25864234	4	31	theme	confocal	523:530	arg1	microscopy					547:556	confocal laser scanning microscopy	523:556	confocal laser scanning microscopy (CLSM)	523:563	After 7 days, the specimens were carefully removed and visualized by confocal laser scanning microscopy (CLSM).
25864234	5	32	theme	COMSTAT	691:697	arg1	software					699:706	COMSTAT software	691:706	COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm)	691:801	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	3	33	theme	sucrose	426:432	arg1	solution					434:441	20% sucrose solution	422:441	20% sucrose solution	422:441	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	3	33	theme	sucrose	426:432	arg1	challenge					411:419	a cariogenic challenge	398:419	a cariogenic challenge (20% sucrose solution, 10x/day)	398:451	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	3	34	theme	cariogenic	400:409	arg1	solution					434:441	20% sucrose solution	422:441	20% sucrose solution	422:441	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	3	34	theme	cariogenic	400:409	arg1	challenge					411:419	a cariogenic challenge	398:419	a cariogenic challenge (20% sucrose solution, 10x/day)	398:451	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	3	35	theme	resin-modified	263:276	arg1	glass-ionomers					295:308	resin-modified and conventional glass-ionomers	263:308	ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam	237:317	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	5	36	dep	cell	633:636	arg1	viability					638:646	viability	638:646	viability	638:646	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	8	37	from	proportion	1223:1232	arg1	volume					1249:1254	the biofilm volume	1237:1254	the biofilm volume	1237:1254	However, qualitatively, glass-ionomer cements and amalgam showed visually a prevalence of non-viable cells forming small clusters distributed by the biofilm, and voids were presented in smaller proportion in the biofilm volume compared to composite and ceramic.
25864234	8	38	theme	biofilm	1241:1247	arg1	volume					1249:1254	the biofilm volume	1237:1254	the biofilm volume	1237:1254	However, qualitatively, glass-ionomer cements and amalgam showed visually a prevalence of non-viable cells forming small clusters distributed by the biofilm, and voids were presented in smaller proportion in the biofilm volume compared to composite and ceramic.
25864234	6	39	theme	statistical	808:818	arg1	analysis					820:827	The statistical analysis	804:827	The statistical analysis	804:827	The statistical analysis was performed by using the Kolmogorov-Smirnov and Kruskal-Wallis tests (P ≤ 5%).
25864234	7	40	theme	statistical	975:985	arg1	difference					987:996	no statistical difference	972:996	no statistical difference regarding different materials	972:1026	RESULTS The medians of the biofilm parameters analyzed showed no statistical difference regarding different materials.
25864234	0	41	theme	restorative	41:51	arg1	materials					53:61	dental restorative materials	34:61	dental restorative materials	34:61	In situ bacterial accumulation on dental restorative materials.
25864234	2	42	theme	restorative	134:144	arg1	materials					146:154	different restorative materials	124:154	different restorative materials	124:154	PURPOSE To evaluate the influence of different restorative materials on the biofilm structure accumulated in situ.
25864234	7	43	theme	parameters	945:954	arg1	medians					922:928	The medians	918:928	RESULTS The medians of the biofilm parameters analyzed	910:963	RESULTS The medians of the biofilm parameters analyzed showed no statistical difference regarding different materials.
25864234	5	44	dep	software	699:706	arg1	area					709:712	area	709:712	area	709:712	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	5	44	dep	software	699:706	arg1	coefficient					775:785	roughness coefficient	765:785	roughness coefficient	765:785	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	5	44	dep	software	699:706	arg1	thickness					751:759	maximum thickness	743:759	maximum thickness	743:759	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	5	44	dep	software	699:706	arg1	bio-volume					715:724	bio-volume	715:724	bio-volume	715:724	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	5	44	dep	software	699:706	arg1	thickness					732:740	mean thickness	727:740	mean thickness	727:740	The images were analyzed qualitatively (descriptive analysis about cell viability and architecture) and quantitatively using COMSTAT software (area, bio-volume, mean thickness, maximum thickness and roughness coefficient of the biofilm).
25864234	3	45	dep	solution	434:441	arg1	10x/day					444:450	10x/day	444:450	10x/day	444:450	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	8	46	dep	glass-ionomer	1053:1065	arg1	cements					1067:1073	cements	1067:1073	cements	1067:1073	However, qualitatively, glass-ionomer cements and amalgam showed visually a prevalence of non-viable cells forming small clusters distributed by the biofilm, and voids were presented in smaller proportion in the biofilm volume compared to composite and ceramic.
25864234	7	47	dep	RESULTS	910:916	arg1	medians					922:928	The medians	918:928	RESULTS The medians of the biofilm parameters analyzed	910:963	RESULTS The medians of the biofilm parameters analyzed showed no statistical difference regarding different materials.
25864234	7	48	theme	biofilm	937:943	arg1	parameters					945:954	the biofilm parameters	933:954	the biofilm parameters analyzed	933:963	RESULTS The medians of the biofilm parameters analyzed showed no statistical difference regarding different materials.
25864234	3	49	theme	palatal	336:342	arg1	devices					344:350	palatal devices	336:350	palatal devices	336:350	METHODS 15 discs of each material (ceramic; resin composite; resin-modified and conventional glass-ionomers; amalgam) were adapted to palatal devices in order to accumulate biofilm in situ, under a cariogenic challenge (20% sucrose solution, 10x/day).
25864234	0	50	from	accumulation	18:29	arg1	materials					53:61	dental restorative materials	34:61	dental restorative materials	34:61	In situ bacterial accumulation on dental restorative materials.
25864234	0	51	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ bacterial accumulation on dental restorative materials.
26906122	0	0	theme	CD44	76:79	arg1	Marker					81:86	CD44 Marker	76:86	CD44 Marker	76:86	Curcumin Induces Apoptosis of Colorectal Cancer Stem Cells by Coupling with CD44 Marker.
26906122	2	1	theme	adenosine	400:408	arg1	monophosphate					410:422	adenosine monophosphate	400:422	adenosine monophosphate (AMP)	400:428	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	1	theme	adenosine	400:408	arg1	AMP					425:427	AMP	425:427	AMP	425:427	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	5	2	theme	glutamine	1216:1224	arg1	transport					1203:1211	the transport	1199:1211	the transport of glutamine into the cells	1199:1239	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	4	3	theme	profile	738:744	arg1	studies					746:752	Further metabolic profile studies	720:752	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells	720:791	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	1	4	theme	colorectal	139:148	arg1	CCSCs					169:173	CCSCs	169:173	CCSCs	169:173	This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism.
26906122	1	4	theme	colorectal	139:148	arg1	cells					162:166	colorectal cancer stem cells	139:166	colorectal cancer stem cells (CCSCs)	139:174	This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism.
26906122	4	5	theme	metabolic	728:736	arg1	studies					746:752	Further metabolic profile studies	720:752	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells	720:791	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	3	6	theme	significant	679:689	arg1	level					691:695	a significant level	677:695	a significant level only in CD44(+) cells	677:717	Our cell culture model study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells.
26906122	4	7	theme	CD44	779:782	arg1	cells					787:791	CD44(-) cells	779:791	CD44(-) cells	779:791	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	1	8	theme	cancer	150:155	arg1	CCSCs					169:173	CCSCs	169:173	CCSCs	169:173	This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism.
26906122	1	8	theme	cancer	150:155	arg1	cells					162:166	colorectal cancer stem cells	139:166	colorectal cancer stem cells (CCSCs)	139:174	This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism.
26906122	5	9	theme	human	1028:1032	arg1	tissues					1034:1040	human tissues	1028:1040	human tissues	1028:1040	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	2	10	theme	adenine	431:437	arg1	composition					385:395	their composition	379:395	their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine	379:527	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	3	11	theme	cell	534:537	arg1	model					547:551	Our cell culture model	530:551	Our cell culture model	530:551	Our cell culture model study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells.
26906122	2	12	theme	threonine	504:512	arg1	composition					385:395	their composition	379:395	their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine	379:527	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	5	13	theme	blocking	1180:1187	arg1	effect					1189:1194	some blocking effect	1175:1194	some blocking effect	1175:1194	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	4	14	theme	hydroxypropionic	856:871	arg1	acid					873:876	hydroxypropionic acid	856:876	hydroxypropionic acid	856:876	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	3	15	dep	treatment	579:587	arg1	μM					593:594	50 μM	590:594	50 μM for 48 h	590:603	Our cell culture model study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells.
26906122	4	16	theme	CD44	766:769	arg1	studies					746:752	Further metabolic profile studies	720:752	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells	720:791	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	5	17	theme	glutamine	1262:1270	arg1	content					1272:1278	the glutamine content	1258:1278	the glutamine content in the CD44(+) cells	1258:1299	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	0	18	with	Coupling	62:69	arg1	Marker					81:86	CD44 Marker	76:86	CD44 Marker	76:86	Curcumin Induces Apoptosis of Colorectal Cancer Stem Cells by Coupling with CD44 Marker.
26906122	2	19	theme	polyp	262:266	arg1	profiles					232:239	the metabolic profiles	218:239	the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue	218:319	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	5	20	from	content	1272:1278	arg1	cells					1295:1299	the CD44(+) cells	1283:1299	the CD44(+) cells	1283:1299	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	2	21	theme	adenomatous	250:260	arg1	polyp					262:266	human adenomatous polyp	244:266	human adenomatous polyp (N = 61)	244:275	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	21	theme	adenomatous	250:260	arg1	N					269:269	N = 61	269:274	N = 61	269:274	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	4	22	theme	glutamine	909:917	arg1	content					919:925	glutamine content	909:925	glutamine content	909:925	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	1	23	theme	stem	157:160	arg1	CCSCs					169:173	CCSCs	169:173	CCSCs	169:173	This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism.
26906122	1	23	theme	stem	157:160	arg1	cells					162:166	colorectal cancer stem cells	139:166	colorectal cancer stem cells (CCSCs)	139:174	This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism.
26906122	4	24	theme	cells	787:791	arg1	studies					746:752	Further metabolic profile studies	720:752	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells	720:791	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	1	25	from	effect	117:122	arg1	mechanism					193:201	its possible mechanism	180:201	its possible mechanism	180:201	This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism.
26906122	1	25	from	effect	117:122	arg1	CCSCs					169:173	CCSCs	169:173	CCSCs	169:173	This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism.
26906122	1	25	from	effect	117:122	arg1	cells					162:166	colorectal cancer stem cells	139:166	colorectal cancer stem cells (CCSCs)	139:174	This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism.
26906122	2	26	theme	human	244:248	arg1	polyp					262:266	human adenomatous polyp	244:266	human adenomatous polyp (N = 61)	244:275	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	26	theme	human	244:248	arg1	N					269:269	N = 61	269:274	N = 61	269:274	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	4	27	theme	curcumin	808:815	arg1	treatment					817:825	curcumin treatment	808:825	curcumin treatment	808:825	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	5	28	theme	cell	1150:1153	arg1	membrane					1155:1162	the cell membrane	1146:1162	the cell membrane	1146:1162	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	4	29	theme	Further	720:726	arg1	studies					746:752	Further metabolic profile studies	720:752	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells	720:791	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	2	30	theme	glutamine	519:527	arg1	composition					385:395	their composition	379:395	their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine	379:527	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	31	theme	=	308:308	arg1	cancer					292:297	colorectal cancer	281:297	colorectal cancer (CRC) (N = 57)	281:312	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	31	theme	=	308:308	arg1	N					306:306	N = 57	306:311	N = 57	306:311	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	32	theme	p	366:366	arg1	<					368:368	p < 0.05	366:373	p < 0.05	366:373	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	32	theme	p	366:366	arg1	differences					353:363	statistically significant differences	327:363	statistically significant differences (p < 0.05)	327:374	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	3	33	theme	CRC	639:641	arg1	cells					643:647	CRC cells	639:647	CRC cells as well as of CCSCs	639:667	Our cell culture model study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells.
26906122	2	34	theme	3-hydroxybutyric	463:478	arg1	acid					480:483	3-hydroxybutyric acid	463:483	3-hydroxybutyric acid	463:483	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	35	theme	=	271:271	arg1	polyp					262:266	human adenomatous polyp	244:266	human adenomatous polyp (N = 61)	244:275	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	35	theme	=	271:271	arg1	N					269:269	N = 61	269:274	N = 61	269:274	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	4	36	theme	CRC	761:763	arg1	studies					746:752	Further metabolic profile studies	720:752	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells	720:791	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	2	37	dep	polyp	262:266	arg1	tissue					314:319	tissue	314:319	tissue	314:319	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	5	38	theme	tissues	1034:1040	arg1	profiles					1016:1023	the metabolic profiles	1002:1023	the metabolic profiles of human tissues and cancer cells	1002:1057	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	2	39	theme	significant	341:351	arg1	<					368:368	p < 0.05	366:373	p < 0.05	366:373	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	39	theme	significant	341:351	arg1	differences					353:363	statistically significant differences	327:363	statistically significant differences (p < 0.05)	327:374	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	0	40	theme	Cancer	41:46	arg1	Cells					53:57	Colorectal Cancer Stem Cells	30:57	Colorectal Cancer Stem Cells	30:57	Curcumin Induces Apoptosis of Colorectal Cancer Stem Cells by Coupling with CD44 Marker.
26906122	2	41	theme	5'-methythioadenosine	440:460	arg1	composition					385:395	their composition	379:395	their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine	379:527	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	5	42	contain	have	1170:1173	arg1	curcumin-CD44					1117:1129	curcumin-CD44	1117:1129	curcumin-CD44	1117:1129	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	5	42	contain	have	1170:1173	arg2	effect					1189:1194	some blocking effect	1175:1194	some blocking effect	1175:1194	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	5	42	contain	have	1170:1173	arg1	coupling					1134:1141	coupling	1134:1141	coupling	1134:1141	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	0	43	theme	Colorectal	30:39	arg1	Cells					53:57	Colorectal Cancer Stem Cells	30:57	Colorectal Cancer Stem Cells	30:57	Curcumin Induces Apoptosis of Colorectal Cancer Stem Cells by Coupling with CD44 Marker.
26906122	3	44	from	level	691:695	arg1	cells					713:717	CD44(+) cells	705:717	CD44(+) cells	705:717	Our cell culture model study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells.
26906122	3	45	theme	culture	539:545	arg1	model					547:551	Our cell culture model	530:551	Our cell culture model	530:551	Our cell culture model study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells.
26906122	1	46	theme	possible	184:191	arg1	mechanism					193:201	its possible mechanism	180:201	its possible mechanism	180:201	This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism.
26906122	0	47	theme	Cells	53:57	arg1	Apoptosis					17:25	Apoptosis	17:25	Apoptosis of Colorectal Cancer Stem Cells	17:57	Curcumin Induces Apoptosis of Colorectal Cancer Stem Cells by Coupling with CD44 Marker.
26906122	5	48	theme	CD44	1287:1290	arg1	cells					1295:1299	the CD44(+) cells	1283:1299	the CD44(+) cells	1283:1299	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	2	49	theme	E2	500:501	arg1	composition					385:395	their composition	379:395	their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine	379:527	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	5	50	dep	have	1170:1173	arg1	decreasing					1247:1256	decreasing	1247:1256	decreasing the glutamine content in the CD44(+) cells	1247:1299	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	5	50	dep	have	1170:1173	arg1	inducing					1305:1312	inducing	1305:1312	inducing apoptosis	1305:1322	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	5	51	from	membrane	1155:1162	arg1	curcumin-CD44					1117:1129	curcumin-CD44	1117:1129	curcumin-CD44	1117:1129	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	0	52	theme	Stem	48:51	arg1	Cells					53:57	Colorectal Cancer Stem Cells	30:57	Colorectal Cancer Stem Cells	30:57	Curcumin Induces Apoptosis of Colorectal Cancer Stem Cells by Coupling with CD44 Marker.
26906122	3	53	theme	cells	643:647	arg1	apoptosis					626:634	apoptosis	626:634	apoptosis of CRC cells as well as of CCSCs	626:667	Our cell culture model study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells.
26906122	2	54	theme	prostaglandin	486:498	arg1	E2					500:501	prostaglandin E2	486:501	prostaglandin E2	486:501	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	55	theme	cancer	292:297	arg1	profiles					232:239	the metabolic profiles	218:239	the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue	218:319	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	3	56	theme	CD44	705:708	arg1	cells					713:717	CD44(+) cells	705:717	CD44(+) cells	705:717	Our cell culture model study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells.
26906122	2	57	theme	colorectal	281:290	arg1	cancer					292:297	colorectal cancer	281:297	colorectal cancer (CRC) (N = 57)	281:312	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	57	theme	colorectal	281:290	arg1	CRC					300:302	CRC	300:302	CRC	300:302	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	57	theme	colorectal	281:290	arg1	N					306:306	N = 57	306:311	N = 57	306:311	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	4	58	theme	CD44	881:884	arg1	cells					889:893	CD44(-) cells	881:893	CD44(-) cells	881:893	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	2	59	theme	acid	480:483	arg1	composition					385:395	their composition	379:395	their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine	379:527	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	2	60	theme	profiles	232:239	arg1	Comparison					204:213	Comparison	204:213	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue	204:319	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	5	61	theme	cells	1053:1057	arg1	profiles					1016:1023	the metabolic profiles	1002:1023	the metabolic profiles of human tissues and cancer cells	1002:1057	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	3	62	theme	curcumin	570:577	arg1	treatment					579:587	curcumin treatment	570:587	curcumin treatment (50 μM for 48 h)	570:604	Our cell culture model study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells.
26906122	2	63	theme	metabolic	222:230	arg1	profiles					232:239	the metabolic profiles	218:239	the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue	218:319	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	4	64	theme	curcumin-treated	935:950	arg1	CRC					952:954	curcumin-treated CRC	935:954	curcumin-treated CRC	935:954	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	1	65	theme	curcumin	127:134	arg1	effect					117:122	the effect	113:122	the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism	113:201	This study investigated the effect of curcumin on colorectal cancer stem cells (CCSCs) and its possible mechanism.
26906122	5	66	theme	cancer	1046:1051	arg1	cells					1053:1057	cancer cells	1046:1057	cancer cells	1046:1057	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	3	67	dep	study	553:557	arg1	found					559:563	found	559:563	study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells	553:717	Our cell culture model study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells.
26906122	2	68	theme	monophosphate	410:422	arg1	composition					385:395	their composition	379:395	their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine	379:527	Comparison of the metabolic profiles of human adenomatous polyp (N = 61) and colorectal cancer (CRC) (N = 57) tissue found statistically significant differences (p < 0.05) in their composition of adenosine monophosphate (AMP), adenine, 5'-methythioadenosine, 3-hydroxybutyric acid, prostaglandin E2, threonine, and glutamine.
26906122	5	69	theme	metabolic	1006:1014	arg1	profiles					1016:1023	the metabolic profiles	1002:1023	the metabolic profiles of human tissues and cancer cells	1002:1057	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	4	70	theme	CD44	960:963	arg1	cells					968:972	CD44(+) cells	960:972	CD44(+) cells	960:972	Further metabolic profile studies of the CRC, CD44(+), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44(+) cells.
26906122	5	71	theme	profiles	1016:1023	arg1	comparison					988:997	our comparison	984:997	our comparison of the metabolic profiles of human tissues and cancer cells	984:1057	Based on our comparison of the metabolic profiles of human tissues and cancer cells, we suggest that curcumin might couple with CD44 and that curcumin-CD44(+) coupling at the cell membrane might have some blocking effect on the transport of glutamine into the cells, thus decreasing the glutamine content in the CD44(+) cells and inducing apoptosis.
26906122	3	72	theme	CCSCs	663:667	arg1	apoptosis					626:634	apoptosis	626:634	apoptosis of CRC cells as well as of CCSCs	626:667	Our cell culture model study found that curcumin treatment (50 μM for 48 h) did indeed increase apoptosis of CRC cells as well as of CCSCs, but at a significant level only in CD44(+) cells.
24592938	8	0	theme	±	1101:1101	arg1	%					1107:1107	1.59 ± 0.06%	1096:1107	1.59 ± 0.06%	1096:1107	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	0	theme	±	1101:1101	arg1	%					1093:1093	0.05%	1089:1093	0.05%	1089:1093	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	2	1	dep	Pomegranate	275:285	arg1	beverages					314:322	yogurt beverages	307:322	yogurt beverages	307:322	Pomegranate (P) and vanilla (V) yogurt beverages were formulated, containing inulin as a prebiotic, along with probiotic bacteria Lactobacillus acidophilus and Bifidobacterium, to produce symbiotic products.
24592938	0	2	theme	sensory	72:78	arg1	properties					80:89	sensory properties	72:89	sensory properties	72:89	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.
24592938	8	3	dep	was	1068:1070	arg1	±					1087:1087	protein: 1.58 ±	1073:1087	protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively	1073:1306	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	9	4	theme	beverage	1412:1419	arg1	flavors					1401:1407	both flavors	1396:1407	both flavors of beverage	1396:1419	Both L. acidophilus and Bifidobacterium were stable and remained above 10(6) CFU/g for both flavors of beverage both with and without carbonation.
24592938	1	5	from	beverage	156:163	arg1	States					179:184	the United States	168:184	the United States	168:184	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24592938	2	6	theme	yogurt	307:312	arg1	beverages					314:322	yogurt beverages	307:322	yogurt beverages	307:322	Pomegranate (P) and vanilla (V) yogurt beverages were formulated, containing inulin as a prebiotic, along with probiotic bacteria Lactobacillus acidophilus and Bifidobacterium, to produce symbiotic products.
24592938	0	7	theme	milk-based	94:103	arg1	beverages					115:123	milk-based symbiotic beverages	94:123	milk-based symbiotic beverages	94:123	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.
24592938	0	8	from	Effects	0:6	arg1	survivability					36:48	probiotic survivability	26:48	probiotic survivability	26:48	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.
24592938	0	8	from	Effects	0:6	arg1	properties					80:89	sensory properties	72:89	sensory properties	72:89	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.
24592938	0	8	from	Effects	0:6	arg1	physicochemical					51:65	physicochemical	51:65	physicochemical	51:65	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.
24592938	8	9	theme	Chemical	1019:1026	arg1	composition					1028:1038	Chemical composition	1019:1038	Chemical composition of the carbonated beverages	1019:1066	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	3	10	theme	high-methoxyl	520:532	arg1	pectin					534:539	high-methoxyl pectin	520:539	high-methoxyl pectin	520:539	These beverages were stabilized with high-methoxyl pectin and whey protein concentrate and compared to samples with approximately 2 volumes of added carbon dioxide (CO2 ).
24592938	10	11	contain	have	1514:1517	arg1	technology					1478:1487	The new manufacturing technology	1456:1487	The new manufacturing technology for these prototypes	1456:1508	The new manufacturing technology for these prototypes may have potential for commercialization of carbonated symbiotic milk-based beverages.
24592938	10	11	contain	have	1514:1517	arg2	potential					1519:1527	potential	1519:1527	potential	1519:1527	The new manufacturing technology for these prototypes may have potential for commercialization of carbonated symbiotic milk-based beverages.
24592938	10	12	theme	carbonated	1554:1563	arg1	beverages					1586:1594	carbonated symbiotic milk-based beverages	1554:1594	carbonated symbiotic milk-based beverages	1554:1594	The new manufacturing technology for these prototypes may have potential for commercialization of carbonated symbiotic milk-based beverages.
24592938	1	13	theme	functional	250:259	arg1	market					267:272	the functional foods market	246:272	the functional foods market	246:272	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24592938	0	14	theme	beverages	115:123	arg1	survivability					36:48	probiotic survivability	26:48	probiotic survivability	26:48	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.
24592938	0	14	theme	beverages	115:123	arg1	properties					80:89	sensory properties	72:89	sensory properties	72:89	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.
24592938	0	14	theme	beverages	115:123	arg1	physicochemical					51:65	physicochemical	51:65	physicochemical	51:65	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.
24592938	8	15	theme	±	1196:1196	arg1	%					1202:1202	0.49 ± 0.02%	1191:1202	0.49 ± 0.02%	1191:1202	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	16	theme	±	1120:1120	arg1	solids					1148:1153	total solids	1142:1153	total solids	1142:1153	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	16	theme	±	1120:1120	arg1	%					1125:1125	1.24 ± 0.2%	1115:1125	1.24 ± 0.2%	1115:1125	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	16	theme	±	1120:1120	arg1	%					1139:1139	1.18 ± 0.11%	1128:1139	1.18 ± 0.11%	1128:1139	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	7	17	from	probiotics	954:963	arg1	viscosity					987:995	viscosity	987:995	viscosity	987:995	Survivability of the probiotics and changes in pH and viscosity were measured weekly.
24592938	7	17	from	probiotics	954:963	arg1	pH					980:981	pH	980:981	pH	980:981	Survivability of the probiotics and changes in pH and viscosity were measured weekly.
24592938	0	18	theme	symbiotic	105:113	arg1	beverages					115:123	milk-based symbiotic beverages	94:123	milk-based symbiotic beverages	94:123	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.
24592938	8	19	dep	±	1087:1087	arg1	%					1183:1183	14.93 ± 0.05%	1171:1183	14.93 ± 0.05%	1171:1183	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	19	dep	±	1087:1087	arg1	%					1107:1107	1.59 ± 0.06%	1096:1107	1.59 ± 0.06%	1096:1107	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	19	dep	±	1087:1087	arg1	ash					1186:1188	ash	1186:1188	ash	1186:1188	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	19	dep	±	1087:1087	arg1	fat					1110:1112	fat	1110:1112	fat	1110:1112	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	19	dep	±	1087:1087	arg1	%					1093:1093	0.05%	1089:1093	0.05%	1089:1093	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	19	dep	±	1087:1087	arg1	%					1280:1280	11.69 ± 0.14%	1268:1280	11.69 ± 0.14% for P and V	1268:1292	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	1	20	theme	United	172:177	arg1	States					179:184	the United States	168:184	the United States	168:184	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24592938	8	21	theme	carbonated	1047:1056	arg1	beverages					1058:1066	the carbonated beverages	1043:1066	the carbonated beverages	1043:1066	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	22	dep	%	1093:1093	arg1	%					1216:1216	0.46 ± 0.03%	1205:1216	0.46 ± 0.03%	1205:1216	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	22	dep	%	1093:1093	arg1	%					1139:1139	1.18 ± 0.11%	1128:1139	1.18 ± 0.11%	1128:1139	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	22	dep	%	1093:1093	arg1	%					1202:1202	0.49 ± 0.02%	1191:1202	0.49 ± 0.02%	1191:1202	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	22	dep	%	1093:1093	arg1	%					1168:1168	14.78 ± 0.11%	1156:1168	14.78 ± 0.11%	1156:1168	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	22	dep	%	1093:1093	arg1	%					1125:1125	1.24 ± 0.2%	1115:1125	1.24 ± 0.2%	1115:1125	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	22	dep	%	1093:1093	arg1	carbohydrate					1223:1234	carbohydrate	1223:1234	carbohydrate	1223:1234	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	22	dep	%	1093:1093	arg1	solids					1148:1153	total solids	1142:1153	total solids	1142:1153	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	22	dep	%	1093:1093	arg1	%					1265:1265	11.47 ± 0.12%	1253:1265	11.47 ± 0.12%	1253:1265	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	3	23	theme	protein	550:556	arg1	concentrate					558:568	whey protein concentrate	545:568	whey protein concentrate	545:568	These beverages were stabilized with high-methoxyl pectin and whey protein concentrate and compared to samples with approximately 2 volumes of added carbon dioxide (CO2 ).
24592938	7	24	from	changes	969:975	arg1	viscosity					987:995	viscosity	987:995	viscosity	987:995	Survivability of the probiotics and changes in pH and viscosity were measured weekly.
24592938	7	24	from	changes	969:975	arg1	pH					980:981	pH	980:981	pH	980:981	Survivability of the probiotics and changes in pH and viscosity were measured weekly.
24592938	8	25	theme	protein	1073:1079	arg1	±					1087:1087	protein: 1.58 ±	1073:1087	protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively	1073:1306	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	1	26	theme	foods	261:265	arg1	market					267:272	the functional foods market	246:272	the functional foods market	246:272	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24592938	2	27	dep	bacteria	396:403	arg1	Bifidobacterium					435:449	Bifidobacterium	435:449	Bifidobacterium	435:449	Pomegranate (P) and vanilla (V) yogurt beverages were formulated, containing inulin as a prebiotic, along with probiotic bacteria Lactobacillus acidophilus and Bifidobacterium, to produce symbiotic products.
24592938	2	27	dep	bacteria	396:403	arg1	acidophilus					419:429	Lactobacillus acidophilus	405:429	Lactobacillus acidophilus	405:429	Pomegranate (P) and vanilla (V) yogurt beverages were formulated, containing inulin as a prebiotic, along with probiotic bacteria Lactobacillus acidophilus and Bifidobacterium, to produce symbiotic products.
24592938	7	28	dep	probiotics	954:963	arg1	the					950:952	the	950:952	the	950:952	Survivability of the probiotics and changes in pH and viscosity were measured weekly.
24592938	2	29	theme	probiotic	386:394	arg1	bacteria					396:403	probiotic bacteria Lactobacillus acidophilus and Bifidobacterium	386:449	probiotic bacteria Lactobacillus acidophilus and Bifidobacterium	386:449	Pomegranate (P) and vanilla (V) yogurt beverages were formulated, containing inulin as a prebiotic, along with probiotic bacteria Lactobacillus acidophilus and Bifidobacterium, to produce symbiotic products.
24592938	10	30	theme	beverages	1586:1594	arg1	commercialization					1533:1549	commercialization	1533:1549	commercialization of carbonated symbiotic milk-based beverages	1533:1594	The new manufacturing technology for these prototypes may have potential for commercialization of carbonated symbiotic milk-based beverages.
24592938	10	31	theme	new	1460:1462	arg1	technology					1478:1487	The new manufacturing technology	1456:1487	The new manufacturing technology for these prototypes	1456:1508	The new manufacturing technology for these prototypes may have potential for commercialization of carbonated symbiotic milk-based beverages.
24592938	7	32	from	pH	980:981	arg1	Survivability					933:945	Survivability	933:945	Survivability of the probiotics and changes in pH and viscosity	933:995	Survivability of the probiotics and changes in pH and viscosity were measured weekly.
24592938	0	33	theme	carbonation	11:21	arg1	Effects					0:6	Effects	0:6	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.	0:124	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.
24592938	8	34	theme	beverages	1058:1066	arg1	composition					1028:1038	Chemical composition	1019:1038	Chemical composition of the carbonated beverages	1019:1066	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	3	35	theme	carbon	632:637	arg1	CO2					648:650	CO2	648:650	CO2	648:650	These beverages were stabilized with high-methoxyl pectin and whey protein concentrate and compared to samples with approximately 2 volumes of added carbon dioxide (CO2 ).
24592938	3	35	theme	carbon	632:637	arg1	dioxide					639:645	added carbon dioxide	626:645	added carbon dioxide (CO2 )	626:652	These beverages were stabilized with high-methoxyl pectin and whey protein concentrate and compared to samples with approximately 2 volumes of added carbon dioxide (CO2 ).
24592938	3	36	theme	whey	545:548	arg1	concentrate					558:568	whey protein concentrate	545:568	whey protein concentrate	545:568	These beverages were stabilized with high-methoxyl pectin and whey protein concentrate and compared to samples with approximately 2 volumes of added carbon dioxide (CO2 ).
24592938	4	37	theme	sealed	678:683	arg1	bottles					691:697	sealed glass bottles	678:697	sealed glass bottles	678:697	Samples were stored in sealed glass bottles at 4 °C for 9 wk for evaluation of physicochemical and functional properties.
24592938	1	38	from	yogurt	236:241	arg1	market					267:272	the functional foods market	246:272	the functional foods market	246:272	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24592938	3	39	theme	dioxide	639:645	arg1	volumes					615:621	approximately 2 volumes	599:621	approximately 2 volumes of added carbon dioxide (CO2 )	599:652	These beverages were stabilized with high-methoxyl pectin and whey protein concentrate and compared to samples with approximately 2 volumes of added carbon dioxide (CO2 ).
24592938	10	40	theme	symbiotic	1565:1573	arg1	beverages					1586:1594	carbonated symbiotic milk-based beverages	1554:1594	carbonated symbiotic milk-based beverages	1554:1594	The new manufacturing technology for these prototypes may have potential for commercialization of carbonated symbiotic milk-based beverages.
24592938	7	41	from	viscosity	987:995	arg1	Survivability					933:945	Survivability	933:945	Survivability of the probiotics and changes in pH and viscosity	933:995	Survivability of the probiotics and changes in pH and viscosity were measured weekly.
24592938	0	42	theme	probiotic	26:34	arg1	survivability					36:48	probiotic survivability	26:48	probiotic survivability	26:48	Effects of carbonation on probiotic survivability, physicochemical, and sensory properties of milk-based symbiotic beverages.
24592938	8	43	theme	±	1177:1177	arg1	%					1093:1093	0.05%	1089:1093	0.05%	1089:1093	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	43	theme	±	1177:1177	arg1	%					1183:1183	14.93 ± 0.05%	1171:1183	14.93 ± 0.05%	1171:1183	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	44	theme	±	1210:1210	arg1	%					1216:1216	0.46 ± 0.03%	1205:1216	0.46 ± 0.03%	1205:1216	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	3	45	with	samples	586:592	arg1	volumes					615:621	approximately 2 volumes	599:621	approximately 2 volumes of added carbon dioxide (CO2 )	599:652	These beverages were stabilized with high-methoxyl pectin and whey protein concentrate and compared to samples with approximately 2 volumes of added carbon dioxide (CO2 ).
24592938	8	46	theme	±	1133:1133	arg1	%					1139:1139	1.18 ± 0.11%	1128:1139	1.18 ± 0.11%	1128:1139	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	46	theme	±	1133:1133	arg1	%					1125:1125	1.24 ± 0.2%	1115:1125	1.24 ± 0.2%	1115:1125	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	6	47	theme	AOAC	919:922	arg1	methods					924:930	standard AOAC methods	910:930	standard AOAC methods	910:930	Physicochemical attributes were analyzed using standard AOAC methods.
24592938	3	48	theme	added	626:630	arg1	CO2					648:650	CO2	648:650	CO2	648:650	These beverages were stabilized with high-methoxyl pectin and whey protein concentrate and compared to samples with approximately 2 volumes of added carbon dioxide (CO2 ).
24592938	3	48	theme	added	626:630	arg1	dioxide					639:645	added carbon dioxide	626:645	added carbon dioxide (CO2 )	626:652	These beverages were stabilized with high-methoxyl pectin and whey protein concentrate and compared to samples with approximately 2 volumes of added carbon dioxide (CO2 ).
24592938	5	49	dep	3	819:819	arg1	replicates					821:830	replicates	821:830	replicates	821:830	Trials were carried out in triplicate and 3 replicates from each trial were analyzed.
24592938	4	50	theme	glass	685:689	arg1	bottles					691:697	sealed glass bottles	678:697	sealed glass bottles	678:697	Samples were stored in sealed glass bottles at 4 °C for 9 wk for evaluation of physicochemical and functional properties.
24592938	6	51	theme	Physicochemical	863:877	arg1	attributes					879:888	Physicochemical attributes	863:888	Physicochemical attributes	863:888	Physicochemical attributes were analyzed using standard AOAC methods.
24592938	6	52	theme	standard	910:917	arg1	methods					924:930	standard AOAC methods	910:930	standard AOAC methods	910:930	Physicochemical attributes were analyzed using standard AOAC methods.
24592938	1	53	theme	Drinkable	126:134	arg1	yogurt					136:141	Drinkable yogurt	126:141	Drinkable yogurt	126:141	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24592938	1	53	theme	Drinkable	126:134	arg1	beverage					156:163	a popular beverage	146:163	a popular beverage in the United States	146:184	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24592938	10	54	theme	milk-based	1575:1584	arg1	beverages					1586:1594	carbonated symbiotic milk-based beverages	1554:1594	carbonated symbiotic milk-based beverages	1554:1594	The new manufacturing technology for these prototypes may have potential for commercialization of carbonated symbiotic milk-based beverages.
24592938	1	55	from	niche	205:209	arg1	States					179:184	the United States	168:184	the United States	168:184	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24592938	8	56	theme	±	1259:1259	arg1	%					1265:1265	11.47 ± 0.12%	1253:1265	11.47 ± 0.12%	1253:1265	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	57	theme	total	1142:1146	arg1	%					1125:1125	1.24 ± 0.2%	1115:1125	1.24 ± 0.2%	1115:1125	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	57	theme	total	1142:1146	arg1	solids					1148:1153	total solids	1142:1153	total solids	1142:1153	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	58	theme	14.93	1171:1175	arg1	%					1093:1093	0.05%	1089:1093	0.05%	1089:1093	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	58	theme	14.93	1171:1175	arg1	%					1183:1183	14.93 ± 0.05%	1171:1183	14.93 ± 0.05%	1171:1183	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	59	theme	±	1162:1162	arg1	%					1168:1168	14.78 ± 0.11%	1156:1168	14.78 ± 0.11%	1156:1168	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	4	60	theme	physicochemical	734:748	arg1	properties					765:774	physicochemical and functional properties	734:774	physicochemical and functional properties	734:774	Samples were stored in sealed glass bottles at 4 °C for 9 wk for evaluation of physicochemical and functional properties.
24592938	7	61	theme	changes	969:975	arg1	Survivability					933:945	Survivability	933:945	Survivability of the probiotics and changes in pH and viscosity	933:995	Survivability of the probiotics and changes in pH and viscosity were measured weekly.
24592938	7	62	from	Survivability	933:945	arg1	viscosity					987:995	viscosity	987:995	viscosity	987:995	Survivability of the probiotics and changes in pH and viscosity were measured weekly.
24592938	7	62	from	Survivability	933:945	arg1	pH					980:981	pH	980:981	pH	980:981	Survivability of the probiotics and changes in pH and viscosity were measured weekly.
24592938	4	63	theme	properties	765:774	arg1	evaluation					720:729	evaluation	720:729	evaluation of physicochemical and functional properties	720:774	Samples were stored in sealed glass bottles at 4 °C for 9 wk for evaluation of physicochemical and functional properties.
24592938	10	64	theme	manufacturing	1464:1476	arg1	technology					1478:1487	The new manufacturing technology	1456:1487	The new manufacturing technology for these prototypes	1456:1508	The new manufacturing technology for these prototypes may have potential for commercialization of carbonated symbiotic milk-based beverages.
24592938	1	65	theme	carbonated	215:224	arg1	yogurt					236:241	carbonated drinkable yogurt	215:241	carbonated drinkable yogurt in the functional foods market	215:272	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24592938	2	66	theme	symbiotic	463:471	arg1	products					473:480	symbiotic products	463:480	symbiotic products	463:480	Pomegranate (P) and vanilla (V) yogurt beverages were formulated, containing inulin as a prebiotic, along with probiotic bacteria Lactobacillus acidophilus and Bifidobacterium, to produce symbiotic products.
24592938	8	67	theme	±	1274:1274	arg1	%					1093:1093	0.05%	1089:1093	0.05%	1089:1093	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	8	67	theme	±	1274:1274	arg1	%					1280:1280	11.69 ± 0.14%	1268:1280	11.69 ± 0.14% for P and V	1268:1292	Chemical composition of the carbonated beverages was: protein: 1.58 ± 0.05%, 1.59 ± 0.06%, fat: 1.24 ± 0.2%, 1.18 ± 0.11%, total solids: 14.78 ± 0.11%, 14.93 ± 0.05%, ash: 0.49 ± 0.02%, 0.46 ± 0.03%, and carbohydrate (by difference): 11.47 ± 0.12%, 11.69 ± 0.14% for P and V, respectively.
24592938	4	68	theme	functional	754:763	arg1	properties					765:774	physicochemical and functional properties	734:774	physicochemical and functional properties	734:774	Samples were stored in sealed glass bottles at 4 °C for 9 wk for evaluation of physicochemical and functional properties.
24592938	7	69	theme	probiotics	954:963	arg1	Survivability					933:945	Survivability	933:945	Survivability of the probiotics and changes in pH and viscosity	933:995	Survivability of the probiotics and changes in pH and viscosity were measured weekly.
24592938	1	70	theme	popular	148:154	arg1	yogurt					136:141	Drinkable yogurt	126:141	Drinkable yogurt	126:141	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24592938	1	70	theme	popular	148:154	arg1	beverage					156:163	a popular beverage	146:163	a popular beverage in the United States	146:184	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24592938	1	71	theme	drinkable	226:234	arg1	yogurt					236:241	carbonated drinkable yogurt	215:241	carbonated drinkable yogurt in the functional foods market	215:272	Drinkable yogurt is a popular beverage in the United States and there may be a niche for carbonated drinkable yogurt in the functional foods market.
24685461	3	0	theme	Au-chit/PLGA-comp	339:355	arg1	NGs					357:359	185nm sized Au-chit/PLGA-comp NGs	327:359	185nm sized Au-chit/PLGA-comp NGs	327:359	185nm sized Au-chit/PLGA-comp NGs, 170nm sized QD-chit/PLGA-comp-NGs and 160nm sized Fe3O4-chit/PLGA-comp-NGs showed RF heating.
24685461	6	1	theme	preliminary	711:721	arg1	data					723:726	The preliminary data	707:726	The preliminary data	707:726	The preliminary data showed that multifaceted chit/PLGA-comp-NGs could be useful for hyperthermia for cancer treatment and microbial labelling and imaging.
24685461	1	2	theme	regeneration	167:178	arg1	method					180:185	regeneration method	167:185	regeneration method	167:185	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs) were prepared by regeneration method and characterized.
24685461	4	3	theme	Candida	586:592	arg1	albicans					594:601	Candida albicans	586:601	Candida albicans	586:601	The QD-chit/PLGA-comp-NGs and 180nm sized umb-chit/PLGA-comp-NGs were well uptaken by Escherichia coli, Staphylococcus aureus and Candida albicans.
24685461	5	4	theme	cancer	691:696	arg1	therapy					698:704	cancer therapy	691:704	cancer therapy	691:704	The chit/PLGA-comp NGs could be useful for microbial monitoring and RF application for cancer therapy.
24685461	3	5	theme	160nm	400:404	arg1	Fe3O4-chit/PLGA-comp-NGs					412:435	160nm sized Fe3O4-chit/PLGA-comp-NGs	400:435	160nm sized Fe3O4-chit/PLGA-comp-NGs	400:435	185nm sized Au-chit/PLGA-comp NGs, 170nm sized QD-chit/PLGA-comp-NGs and 160nm sized Fe3O4-chit/PLGA-comp-NGs showed RF heating.
24685461	4	6	theme	QD-chit/PLGA-comp-NGs	460:480	arg1	umb-chit/PLGA-comp-NGs					498:519	The QD-chit/PLGA-comp-NGs and 180nm sized umb-chit/PLGA-comp-NGs	456:519	The QD-chit/PLGA-comp-NGs and 180nm sized umb-chit/PLGA-comp-NGs	456:519	The QD-chit/PLGA-comp-NGs and 180nm sized umb-chit/PLGA-comp-NGs were well uptaken by Escherichia coli, Staphylococcus aureus and Candida albicans.
24685461	1	7	theme	Cyto-compatible	70:84	arg1	NGs					145:147	chit/PLGA-comp NGs	130:147	chit/PLGA-comp NGs	130:147	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs) were prepared by regeneration method and characterized.
24685461	1	7	theme	Cyto-compatible	70:84	arg1	nanogels					120:127	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels	70:127	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs)	70:148	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs) were prepared by regeneration method and characterized.
24685461	3	8	theme	sized	368:372	arg1	QD-chit/PLGA-comp-NGs					374:394	170nm sized QD-chit/PLGA-comp-NGs	362:394	170nm sized QD-chit/PLGA-comp-NGs	362:394	185nm sized Au-chit/PLGA-comp NGs, 170nm sized QD-chit/PLGA-comp-NGs and 160nm sized Fe3O4-chit/PLGA-comp-NGs showed RF heating.
24685461	6	9	theme	cancer	809:814	arg1	treatment					816:824	cancer treatment	809:824	cancer treatment	809:824	The preliminary data showed that multifaceted chit/PLGA-comp-NGs could be useful for hyperthermia for cancer treatment and microbial labelling and imaging.
24685461	5	10	theme	microbial	647:655	arg1	monitoring					657:666	microbial monitoring	647:666	microbial monitoring	647:666	The chit/PLGA-comp NGs could be useful for microbial monitoring and RF application for cancer therapy.
24685461	0	11	theme	Multifaceted	0:11	arg1	lactic-co-glycolic					25:42	lactic-co-glycolic	25:42	lactic-co-glycolic	25:42	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels.
24685461	0	11	theme	Multifaceted	0:11	arg1	chitin/poly					13:23	Multifaceted chitin/poly	0:23	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels.	0:68	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels.
24685461	0	12	theme	chitin/poly	13:23	arg1	nanogels					60:67	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels	0:67	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels.	0:68	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels.
24685461	3	13	theme	RF	444:445	arg1	heating					447:453	RF heating	444:453	RF heating	444:453	185nm sized Au-chit/PLGA-comp NGs, 170nm sized QD-chit/PLGA-comp-NGs and 160nm sized Fe3O4-chit/PLGA-comp-NGs showed RF heating.
24685461	1	14	theme	chitin/PLGA	98:108	arg1	NGs					145:147	chit/PLGA-comp NGs	130:147	chit/PLGA-comp NGs	130:147	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs) were prepared by regeneration method and characterized.
24685461	1	14	theme	chitin/PLGA	98:108	arg1	nanogels					120:127	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels	70:127	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs)	70:148	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs) were prepared by regeneration method and characterized.
24685461	3	15	theme	sized	406:410	arg1	Fe3O4-chit/PLGA-comp-NGs					412:435	160nm sized Fe3O4-chit/PLGA-comp-NGs	400:435	160nm sized Fe3O4-chit/PLGA-comp-NGs	400:435	185nm sized Au-chit/PLGA-comp NGs, 170nm sized QD-chit/PLGA-comp-NGs and 160nm sized Fe3O4-chit/PLGA-comp-NGs showed RF heating.
24685461	5	16	theme	RF	672:673	arg1	application					675:685	RF application	672:685	RF application	672:685	The chit/PLGA-comp NGs could be useful for microbial monitoring and RF application for cancer therapy.
24685461	1	17	theme	composite	110:118	arg1	NGs					145:147	chit/PLGA-comp NGs	130:147	chit/PLGA-comp NGs	130:147	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs) were prepared by regeneration method and characterized.
24685461	1	17	theme	composite	110:118	arg1	nanogels					120:127	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels	70:127	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs)	70:148	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs) were prepared by regeneration method and characterized.
24685461	0	18	theme	acid	45:48	arg1	nanogels					60:67	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels	0:67	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels.	0:68	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels.
24685461	2	19	dep	modified	255:262	arg1	surface					247:253	surface	247:253	surface	247:253	The multifaceted chit/PLGA-comp NGs were surface modified with Au, Fe3O4, CdTe/ZnTe-QDs and umbelliferone, respectively.
24685461	3	20	theme	185nm	327:331	arg1	NGs					357:359	185nm sized Au-chit/PLGA-comp NGs	327:359	185nm sized Au-chit/PLGA-comp NGs	327:359	185nm sized Au-chit/PLGA-comp NGs, 170nm sized QD-chit/PLGA-comp-NGs and 160nm sized Fe3O4-chit/PLGA-comp-NGs showed RF heating.
24685461	0	21	theme	composite	50:58	arg1	nanogels					60:67	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels	0:67	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels.	0:68	Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels.
24685461	3	22	theme	sized	333:337	arg1	NGs					357:359	185nm sized Au-chit/PLGA-comp NGs	327:359	185nm sized Au-chit/PLGA-comp NGs	327:359	185nm sized Au-chit/PLGA-comp NGs, 170nm sized QD-chit/PLGA-comp-NGs and 160nm sized Fe3O4-chit/PLGA-comp-NGs showed RF heating.
24685461	4	23	theme	180nm	486:490	arg1	umb-chit/PLGA-comp-NGs					498:519	The QD-chit/PLGA-comp-NGs and 180nm sized umb-chit/PLGA-comp-NGs	456:519	The QD-chit/PLGA-comp-NGs and 180nm sized umb-chit/PLGA-comp-NGs	456:519	The QD-chit/PLGA-comp-NGs and 180nm sized umb-chit/PLGA-comp-NGs were well uptaken by Escherichia coli, Staphylococcus aureus and Candida albicans.
24685461	2	24	mod	modified	255:262	arg3	CdTe/ZnTe-QDs					280:292	CdTe/ZnTe-QDs	280:292	CdTe/ZnTe-QDs	280:292	The multifaceted chit/PLGA-comp NGs were surface modified with Au, Fe3O4, CdTe/ZnTe-QDs and umbelliferone, respectively.
24685461	2	24	mod	modified	255:262	arg3	umbelliferone					298:310	umbelliferone	298:310	umbelliferone	298:310	The multifaceted chit/PLGA-comp NGs were surface modified with Au, Fe3O4, CdTe/ZnTe-QDs and umbelliferone, respectively.
24685461	2	24	mod	modified	255:262	arg3	Au					269:270	Au	269:270	Au	269:270	The multifaceted chit/PLGA-comp NGs were surface modified with Au, Fe3O4, CdTe/ZnTe-QDs and umbelliferone, respectively.
24685461	2	24	mod	modified	255:262	arg3	Fe3O4					273:277	Fe3O4	273:277	Fe3O4	273:277	The multifaceted chit/PLGA-comp NGs were surface modified with Au, Fe3O4, CdTe/ZnTe-QDs and umbelliferone, respectively.
24685461	2	24	mod	modified	255:262	arg1	NGs					238:240	The multifaceted chit/PLGA-comp NGs	206:240	The multifaceted chit/PLGA-comp NGs	206:240	The multifaceted chit/PLGA-comp NGs were surface modified with Au, Fe3O4, CdTe/ZnTe-QDs and umbelliferone, respectively.
24685461	1	25	theme	chit/PLGA-comp	130:143	arg1	NGs					145:147	chit/PLGA-comp NGs	130:147	chit/PLGA-comp NGs	130:147	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs) were prepared by regeneration method and characterized.
24685461	1	25	theme	chit/PLGA-comp	130:143	arg1	nanogels					120:127	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels	70:127	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs)	70:148	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs) were prepared by regeneration method and characterized.
24685461	2	26	theme	chit/PLGA-comp	223:236	arg1	NGs					238:240	The multifaceted chit/PLGA-comp NGs	206:240	The multifaceted chit/PLGA-comp NGs	206:240	The multifaceted chit/PLGA-comp NGs were surface modified with Au, Fe3O4, CdTe/ZnTe-QDs and umbelliferone, respectively.
24685461	6	27	theme	multifaceted	740:751	arg1	chit/PLGA-comp-NGs					753:770	multifaceted chit/PLGA-comp-NGs	740:770	multifaceted chit/PLGA-comp-NGs	740:770	The preliminary data showed that multifaceted chit/PLGA-comp-NGs could be useful for hyperthermia for cancer treatment and microbial labelling and imaging.
24685461	2	28	theme	multifaceted	210:221	arg1	NGs					238:240	The multifaceted chit/PLGA-comp NGs	206:240	The multifaceted chit/PLGA-comp NGs	206:240	The multifaceted chit/PLGA-comp NGs were surface modified with Au, Fe3O4, CdTe/ZnTe-QDs and umbelliferone, respectively.
24685461	6	29	theme	microbial	830:838	arg1	labelling					840:848	microbial labelling	830:848	microbial labelling	830:848	The preliminary data showed that multifaceted chit/PLGA-comp-NGs could be useful for hyperthermia for cancer treatment and microbial labelling and imaging.
24685461	1	30	dep	Cyto-compatible	70:84	arg1	sized					92:96	sized	92:96	sized	92:96	Cyto-compatible, 80nm sized chitin/PLGA composite nanogels (chit/PLGA-comp NGs) were prepared by regeneration method and characterized.
24685461	4	31	theme	sized	492:496	arg1	umb-chit/PLGA-comp-NGs					498:519	The QD-chit/PLGA-comp-NGs and 180nm sized umb-chit/PLGA-comp-NGs	456:519	The QD-chit/PLGA-comp-NGs and 180nm sized umb-chit/PLGA-comp-NGs	456:519	The QD-chit/PLGA-comp-NGs and 180nm sized umb-chit/PLGA-comp-NGs were well uptaken by Escherichia coli, Staphylococcus aureus and Candida albicans.
24685461	3	32	theme	170nm	362:366	arg1	QD-chit/PLGA-comp-NGs					374:394	170nm sized QD-chit/PLGA-comp-NGs	362:394	170nm sized QD-chit/PLGA-comp-NGs	362:394	185nm sized Au-chit/PLGA-comp NGs, 170nm sized QD-chit/PLGA-comp-NGs and 160nm sized Fe3O4-chit/PLGA-comp-NGs showed RF heating.
24685461	5	33	theme	chit/PLGA-comp	608:621	arg1	NGs					623:625	The chit/PLGA-comp NGs	604:625	The chit/PLGA-comp NGs	604:625	The chit/PLGA-comp NGs could be useful for microbial monitoring and RF application for cancer therapy.
24685461	5	33	theme	chit/PLGA-comp	608:621	arg1	useful					636:641	useful	636:641	useful	636:641	The chit/PLGA-comp NGs could be useful for microbial monitoring and RF application for cancer therapy.
26423292	0	0	theme	lime	76:79	arg1	mud					81:83	lime mud	76:83	lime mud purified by sucrose method	76:110	Study of CO2 cyclic absorption stability of CaO-based sorbents derived from lime mud purified by sucrose method.
26423292	2	1	theme	cyclic	441:446	arg1	stability					459:467	CO2 cyclic absorption stability	437:467	CO2 cyclic absorption stability	437:467	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability were illustrated.
26423292	2	2	theme	CO2	437:439	arg1	stability					459:467	CO2 cyclic absorption stability	437:467	CO2 cyclic absorption stability	437:467	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability were illustrated.
26423292	6	3	theme	phase	883:887	arg1	Occurrence					857:866	Occurrence	857:866	Occurrence of Ca12Al14O33 phase during precalcination	857:909	Occurrence of Ca12Al14O33 phase during precalcination was probably responsible for the excellent CO2 cyclic stability.
26423292	1	4	from	production	282:291	arg1	BTs					263:265	BTs	263:265	BTs	263:265	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	1	4	from	production	282:291	arg1	rejects-bauxite-tailings					237:260	mineral rejects-bauxite-tailings	229:260	mineral rejects-bauxite-tailings (BTs) from aluminum production	229:291	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	6	5	theme	Ca12Al14O33	871:881	arg1	phase					883:887	Ca12Al14O33 phase	871:887	Ca12Al14O33 phase	871:887	Occurrence of Ca12Al14O33 phase during precalcination was probably responsible for the excellent CO2 cyclic stability.
26423292	1	6	theme	CaO-based	308:316	arg1	sorbents					318:325	the CaO-based sorbents	304:325	the CaO-based sorbents for high-temperature CO2 capture	304:358	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	0	7	theme	sucrose	97:103	arg1	method					105:110	sucrose method	97:110	sucrose method	97:110	Study of CO2 cyclic absorption stability of CaO-based sorbents derived from lime mud purified by sucrose method.
26423292	3	8	theme	cyclic	491:496	arg1	behavior					510:517	The cyclic carbonation behavior	487:517	The cyclic carbonation behavior	487:517	The cyclic carbonation behavior was investigated in a thermogravimetric analyzer (TGA).
26423292	5	9	theme	CO2	744:746	arg1	conversion					777:786	a superior CO2 cyclic absorption-desorption conversion	733:786	a superior CO2 cyclic absorption-desorption conversion	733:786	The results reflected that the as-synthesized CaO-based sorbent doped with 10 wt% BTs showed a superior CO2 cyclic absorption-desorption conversion during multiple cycles, with conversion being >38 % after 50 cycles.
26423292	5	10	theme	wt	718:719	arg1	BTs					722:724	10 wt% BTs	715:724	10 wt% BTs	715:724	The results reflected that the as-synthesized CaO-based sorbent doped with 10 wt% BTs showed a superior CO2 cyclic absorption-desorption conversion during multiple cycles, with conversion being >38 % after 50 cycles.
26423292	5	11	theme	superior	735:742	arg1	conversion					777:786	a superior CO2 cyclic absorption-desorption conversion	733:786	a superior CO2 cyclic absorption-desorption conversion	733:786	The results reflected that the as-synthesized CaO-based sorbent doped with 10 wt% BTs showed a superior CO2 cyclic absorption-desorption conversion during multiple cycles, with conversion being >38 % after 50 cycles.
26423292	3	12	theme	carbonation	498:508	arg1	behavior					510:517	The cyclic carbonation behavior	487:517	The cyclic carbonation behavior	487:517	The cyclic carbonation behavior was investigated in a thermogravimetric analyzer (TGA).
26423292	2	13	theme	time	412:415	arg1	Effects					375:381	Effects	375:381	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability	375:467	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability were illustrated.
26423292	1	14	theme	high-temperature	331:346	arg1	capture					352:358	high-temperature CO2 capture	331:358	high-temperature CO2 capture	331:358	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	6	15	theme	cyclic	958:963	arg1	stability					965:973	the excellent CO2 cyclic stability	940:973	the excellent CO2 cyclic stability	940:973	Occurrence of Ca12Al14O33 phase during precalcination was probably responsible for the excellent CO2 cyclic stability.
26423292	1	16	theme	sucrose	145:151	arg1	method					153:158	sucrose method	145:158	sucrose method	145:158	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	2	17	theme	precalcining	399:410	arg1	time					412:415	precalcining time	399:415	precalcining time	399:415	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability were illustrated.
26423292	1	18	theme	mineral	229:235	arg1	BTs					263:265	BTs	263:265	BTs	263:265	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	1	18	theme	mineral	229:235	arg1	rejects-bauxite-tailings					237:260	mineral rejects-bauxite-tailings	229:260	mineral rejects-bauxite-tailings (BTs) from aluminum production	229:291	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	3	19	theme	thermogravimetric	541:557	arg1	TGA					569:571	TGA	569:571	TGA	569:571	The cyclic carbonation behavior was investigated in a thermogravimetric analyzer (TGA).
26423292	3	19	theme	thermogravimetric	541:557	arg1	analyzer					559:566	a thermogravimetric analyzer	539:566	a thermogravimetric analyzer (TGA)	539:572	The cyclic carbonation behavior was investigated in a thermogravimetric analyzer (TGA).
26423292	0	20	theme	cyclic	13:18	arg1	stability					31:39	CO2 cyclic absorption stability	9:39	CO2 cyclic absorption stability of CaO-based sorbents	9:61	Study of CO2 cyclic absorption stability of CaO-based sorbents derived from lime mud purified by sucrose method.
26423292	6	21	theme	CO2	954:956	arg1	stability					965:973	the excellent CO2 cyclic stability	940:973	the excellent CO2 cyclic stability	940:973	Occurrence of Ca12Al14O33 phase during precalcination was probably responsible for the excellent CO2 cyclic stability.
26423292	5	22	theme	cyclic	748:753	arg1	conversion					777:786	a superior CO2 cyclic absorption-desorption conversion	733:786	a superior CO2 cyclic absorption-desorption conversion	733:786	The results reflected that the as-synthesized CaO-based sorbent doped with 10 wt% BTs showed a superior CO2 cyclic absorption-desorption conversion during multiple cycles, with conversion being >38 % after 50 cycles.
26423292	2	23	theme	BTs	386:388	arg1	content					390:396	BTs content	386:396	BTs content	386:396	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability were illustrated.
26423292	0	24	theme	CO2	9:11	arg1	stability					31:39	CO2 cyclic absorption stability	9:39	CO2 cyclic absorption stability of CaO-based sorbents	9:61	Study of CO2 cyclic absorption stability of CaO-based sorbents derived from lime mud purified by sucrose method.
26423292	6	25	theme	excellent	944:952	arg1	stability					965:973	the excellent CO2 cyclic stability	940:973	the excellent CO2 cyclic stability	940:973	Occurrence of Ca12Al14O33 phase during precalcination was probably responsible for the excellent CO2 cyclic stability.
26423292	5	26	theme	as-synthesized	671:684	arg1	sorbent					696:702	the as-synthesized CaO-based sorbent	667:702	the as-synthesized CaO-based sorbent doped with 10 wt% BTs	667:724	The results reflected that the as-synthesized CaO-based sorbent doped with 10 wt% BTs showed a superior CO2 cyclic absorption-desorption conversion during multiple cycles, with conversion being >38 % after 50 cycles.
26423292	1	27	theme	CO2	348:350	arg1	capture					352:358	high-temperature CO2 capture	331:358	high-temperature CO2 capture	331:358	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	0	28	theme	stability	31:39	arg1	Study					0:4	Study	0:4	Study of CO2 cyclic absorption stability of CaO-based sorbents	0:61	Study of CO2 cyclic absorption stability of CaO-based sorbents derived from lime mud purified by sucrose method.
26423292	5	29	theme	CaO-based	686:694	arg1	sorbent					696:702	the as-synthesized CaO-based sorbent	667:702	the as-synthesized CaO-based sorbent doped with 10 wt% BTs	667:724	The results reflected that the as-synthesized CaO-based sorbent doped with 10 wt% BTs showed a superior CO2 cyclic absorption-desorption conversion during multiple cycles, with conversion being >38 % after 50 cycles.
26423292	0	30	theme	absorption	20:29	arg1	stability					31:39	CO2 cyclic absorption stability	9:39	CO2 cyclic absorption stability of CaO-based sorbents	9:61	Study of CO2 cyclic absorption stability of CaO-based sorbents derived from lime mud purified by sucrose method.
26423292	2	31	theme	temperature	422:432	arg1	Effects					375:381	Effects	375:381	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability	375:467	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability were illustrated.
26423292	5	32	theme	multiple	795:802	arg1	cycles					804:809	multiple cycles	795:809	multiple cycles	795:809	The results reflected that the as-synthesized CaO-based sorbent doped with 10 wt% BTs showed a superior CO2 cyclic absorption-desorption conversion during multiple cycles, with conversion being >38 % after 50 cycles.
26423292	1	33	theme	paper-making	174:185	arg1	industry					187:194	paper-making industry	174:194	paper-making industry	174:194	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	0	34	theme	CaO-based	44:52	arg1	sorbents					54:61	CaO-based sorbents	44:61	CaO-based sorbents	44:61	Study of CO2 cyclic absorption stability of CaO-based sorbents derived from lime mud purified by sucrose method.
26423292	2	35	theme	absorption	448:457	arg1	stability					459:467	CO2 cyclic absorption stability	437:467	CO2 cyclic absorption stability	437:467	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability were illustrated.
26423292	5	36	theme	absorption-desorption	755:775	arg1	conversion					777:786	a superior CO2 cyclic absorption-desorption conversion	733:786	a superior CO2 cyclic absorption-desorption conversion	733:786	The results reflected that the as-synthesized CaO-based sorbent doped with 10 wt% BTs showed a superior CO2 cyclic absorption-desorption conversion during multiple cycles, with conversion being >38 % after 50 cycles.
26423292	1	37	theme	aluminum	273:280	arg1	production					282:291	aluminum production	273:291	aluminum production	273:291	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	1	38	attach	derived	161:167	arg1	industry					187:194	paper-making industry	174:194	paper-making industry	174:194	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	1	38	attach	derived	161:167	arg2	method					153:158	sucrose method	145:158	sucrose method	145:158	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	4	39	theme	Phase	575:579	arg1	composition					581:591	Phase composition	575:591	Phase composition	575:591	Phase composition and morphologies were analyzed by XRD and SEM.
26423292	2	40	from	Effects	375:381	arg1	stability					459:467	CO2 cyclic absorption stability	437:467	CO2 cyclic absorption stability	437:467	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability were illustrated.
26423292	1	41	theme	lime	119:122	arg1	LM					129:130	LM	129:130	LM	129:130	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	1	41	theme	lime	119:122	arg1	mud					124:126	lime mud	119:126	lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor	119:216	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
26423292	5	42	theme	%	720:720	arg1	BTs					722:724	10 wt% BTs	715:724	10 wt% BTs	715:724	The results reflected that the as-synthesized CaO-based sorbent doped with 10 wt% BTs showed a superior CO2 cyclic absorption-desorption conversion during multiple cycles, with conversion being >38 % after 50 cycles.
26423292	0	43	theme	sorbents	54:61	arg1	stability					31:39	CO2 cyclic absorption stability	9:39	CO2 cyclic absorption stability of CaO-based sorbents	9:61	Study of CO2 cyclic absorption stability of CaO-based sorbents derived from lime mud purified by sucrose method.
26423292	2	44	theme	content	390:396	arg1	Effects					375:381	Effects	375:381	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability	375:467	Effects of BTs content, precalcining time, and temperature on CO2 cyclic absorption stability were illustrated.
26423292	1	45	theme	calcium	200:206	arg1	precursor					208:216	calcium precursor	200:216	calcium precursor	200:216	Using lime mud (LM) purified by sucrose method, derived from paper-making industry, as calcium precursor, and using mineral rejects-bauxite-tailings (BTs) from aluminum production as dopant, the CaO-based sorbents for high-temperature CO2 capture were prepared.
27223133	3	0	from	effect	373:378	arg1	composition					432:442	Streptococcus mutans biofilm composition	403:442	Streptococcus mutans biofilm composition	403:442	The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization.
27223133	3	0	from	effect	373:378	arg1	enamel					448:453	enamel	448:453	enamel	448:453	The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization.
27223133	9	1	theme	p	1445:1445	arg1	demineralization					1427:1442	higher demineralization	1420:1442	higher demineralization (p < 0.0001) on either enamel or dentine	1420:1483	Biofilms exposed to starch + sucrose were more acidogenic and caused higher demineralization (p < 0.0001) on either enamel or dentine than those exposed to each carbohydrate alone.
27223133	9	1	theme	p	1445:1445	arg1	<					1447:1447	p < 0.0001	1445:1454	p < 0.0001	1445:1454	Biofilms exposed to starch + sucrose were more acidogenic and caused higher demineralization (p < 0.0001) on either enamel or dentine than those exposed to each carbohydrate alone.
27223133	8	2	theme	surface	1328:1334	arg1	loss					1345:1348	surface hardness loss	1328:1348	surface hardness loss	1328:1348	Dentine and enamel demineralization was assessed by determining the percentage of surface hardness loss.
27223133	1	3	theme	cariogenic	124:133	arg1	Sucrose					104:110	Sucrose	104:110	Sucrose	104:110	Sucrose is the most cariogenic dietary carbohydrate and starch is considered non-cariogenic for enamel and moderately cariogenic for dentine.
27223133	1	3	theme	cariogenic	124:133	arg1	carbohydrate					143:154	the most cariogenic dietary carbohydrate	115:154	the most cariogenic dietary carbohydrate	115:154	Sucrose is the most cariogenic dietary carbohydrate and starch is considered non-cariogenic for enamel and moderately cariogenic for dentine.
27223133	0	4	theme	sucrose	67:73	arg1	combination					41:51	a combination	39:51	a combination of starch and sucrose	39:73	Enamel and dentine demineralization by a combination of starch and sucrose in a biofilm - caries model.
27223133	1	5	theme	dietary	135:141	arg1	Sucrose					104:110	Sucrose	104:110	Sucrose	104:110	Sucrose is the most cariogenic dietary carbohydrate and starch is considered non-cariogenic for enamel and moderately cariogenic for dentine.
27223133	1	5	theme	dietary	135:141	arg1	carbohydrate					143:154	the most cariogenic dietary carbohydrate	115:154	the most cariogenic dietary carbohydrate	115:154	Sucrose is the most cariogenic dietary carbohydrate and starch is considered non-cariogenic for enamel and moderately cariogenic for dentine.
27223133	0	6	theme	biofilm	80:86	arg1	model					97:101	a biofilm - caries model	78:101	a biofilm - caries model	78:101	Enamel and dentine demineralization by a combination of starch and sucrose in a biofilm - caries model.
27223133	0	7	from	Enamel	0:5	arg1	model					97:101	a biofilm - caries model	78:101	a biofilm - caries model	78:101	Enamel and dentine demineralization by a combination of starch and sucrose in a biofilm - caries model.
27223133	8	8	dep	Dentine	1246:1252	arg1	demineralization					1265:1280	demineralization	1265:1280	demineralization	1265:1280	Dentine and enamel demineralization was assessed by determining the percentage of surface hardness loss.
27223133	7	9	theme	polysaccharide	1206:1219	arg1	content					1221:1227	polysaccharide content	1206:1227	polysaccharide content	1206:1227	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	7	10	theme	viable	1174:1179	arg1	count					1195:1199	viable microorganism count	1174:1199	viable microorganism count	1174:1199	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	9	11	from	demineralization	1427:1442	arg1	dentine					1477:1483	dentine	1477:1483	dentine	1477:1483	Biofilms exposed to starch + sucrose were more acidogenic and caused higher demineralization (p < 0.0001) on either enamel or dentine than those exposed to each carbohydrate alone.
27223133	9	11	from	demineralization	1427:1442	arg1	enamel					1467:1472	enamel	1467:1472	enamel	1467:1472	Biofilms exposed to starch + sucrose were more acidogenic and caused higher demineralization (p < 0.0001) on either enamel or dentine than those exposed to each carbohydrate alone.
27223133	4	12	theme	culture	569:575	arg1	medium					577:582	culture medium	569:582	culture medium containing 10% saliva	569:604	Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva.
27223133	0	13	theme	caries	90:95	arg1	model					97:101	a biofilm - caries model	78:101	a biofilm - caries model	78:101	Enamel and dentine demineralization by a combination of starch and sucrose in a biofilm - caries model.
27223133	5	14	dep	exposed	617:623	arg1	times/day					628:636	8 times/day	626:636	8 times/day	626:636	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	3	15	theme	Streptococcus	403:415	arg1	composition					432:442	Streptococcus mutans biofilm composition	403:442	Streptococcus mutans biofilm composition	403:442	The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization.
27223133	5	16	theme	negative	686:693	arg1	NaCl					680:683	0.9% NaCl	675:683	0.9% NaCl (negative control)	675:702	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	16	theme	negative	686:693	arg1	control					695:701	negative control	686:701	negative control	686:701	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	8	17	theme	hardness	1336:1343	arg1	loss					1345:1348	surface hardness loss	1328:1348	surface hardness loss	1328:1348	Dentine and enamel demineralization was assessed by determining the percentage of surface hardness loss.
27223133	7	18	theme	culture	1044:1050	arg1	medium					1052:1057	the culture medium pH.	1040:1061	(2 times/day) the culture medium pH.	1026:1061	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	7	18	theme	culture	1044:1050	arg1	times/day					1029:1037	2 times/day	1027:1037	2 times/day	1027:1037	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	2	19	theme	sucrose	306:312	arg1	combination					280:290	the combination	276:290	the combination of starch and sucrose	276:312	However, the cariogenicity of the combination of starch and sucrose remains unclear.
27223133	5	20	theme	%	748:748	arg1	treatments					663:672	the following treatments	649:672	the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose)	649:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	20	theme	%	748:748	arg1	sucrose					750:756	10% sucrose	746:756	10% sucrose (starch + sucrose)	746:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	21	theme	%	718:718	arg1	sucrose					720:726	10% sucrose	716:726	10% sucrose	716:726	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	21	theme	%	718:718	arg1	treatments					663:672	the following treatments	649:672	the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose)	649:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	22	theme	1	705:705	arg1	%					706:706	%	706:706	%	706:706	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	7	23	theme	microorganism	1181:1193	arg1	count					1195:1199	viable microorganism count	1174:1199	viable microorganism count	1174:1199	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	3	24	theme	combination	388:398	arg1	effect					373:378	the effect	369:378	the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization	369:482	The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization.
27223133	6	25	theme	starch	834:839	arg1	metabolization					841:854	the starch metabolization	830:854	the starch metabolization	830:854	To simulate the effect of human salivary amylase on the starch metabolization, the biofilms were pretreated with saliva before each treatment and saliva was also added to the culture medium.
27223133	5	26	dep	treatments	663:672	arg1	treatments					663:672	the following treatments	649:672	the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose)	649:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	26	dep	treatments	663:672	arg1	control					695:701	negative control	686:701	negative control	686:701	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	26	dep	treatments	663:672	arg1	starch					735:740	1% starch	732:740	1% starch	732:740	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	26	dep	treatments	663:672	arg1	sucrose					720:726	10% sucrose	716:726	10% sucrose	716:726	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	26	dep	treatments	663:672	arg1	NaCl					680:683	0.9% NaCl	675:683	0.9% NaCl (negative control)	675:702	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	26	dep	treatments	663:672	arg1	sucrose					750:756	10% sucrose	746:756	10% sucrose (starch + sucrose)	746:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	26	dep	treatments	663:672	arg1	starch					708:713	1% starch	705:713	1% starch	705:713	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	2	27	theme	starch	295:300	arg1	combination					280:290	the combination	276:290	the combination of starch and sucrose	276:312	However, the cariogenicity of the combination of starch and sucrose remains unclear.
27223133	5	28	theme	%	678:678	arg1	treatments					663:672	the following treatments	649:672	the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose)	649:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	28	theme	%	678:678	arg1	NaCl					680:683	0.9% NaCl	675:683	0.9% NaCl (negative control)	675:702	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	28	theme	%	678:678	arg1	control					695:701	negative control	686:701	negative control	686:701	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	3	29	theme	study	347:351	arg1	aim					335:337	The aim	331:337	The aim of this study	331:351	The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization.
27223133	4	30	theme	saliva-coated	527:539	arg1	enamel					541:546	saliva-coated enamel	527:546	saliva-coated enamel	527:546	Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva.
27223133	5	31	theme	following	653:661	arg1	treatments					663:672	the following treatments	649:672	the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose)	649:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	31	theme	following	653:661	arg1	starch					735:740	1% starch	732:740	1% starch	732:740	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	31	theme	following	653:661	arg1	sucrose					720:726	10% sucrose	716:726	10% sucrose	716:726	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	31	theme	following	653:661	arg1	NaCl					680:683	0.9% NaCl	675:683	0.9% NaCl (negative control)	675:702	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	31	theme	following	653:661	arg1	sucrose					750:756	10% sucrose	746:756	10% sucrose (starch + sucrose)	746:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	31	theme	following	653:661	arg1	starch					708:713	1% starch	705:713	1% starch	705:713	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	10	32	theme	sucrose	1603:1609	arg1	potential					1590:1598	the cariogenic potential	1575:1598	the cariogenic potential of sucrose	1575:1609	The findings suggest that starch increases the cariogenic potential of sucrose.
27223133	5	33	theme	1	732:732	arg1	%					733:733	%	733:733	%	733:733	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	0	34	theme	dentine	11:17	arg1	demineralization					19:34	dentine demineralization	11:34	dentine demineralization	11:34	Enamel and dentine demineralization by a combination of starch and sucrose in a biofilm - caries model.
27223133	4	35	theme	dentine	552:558	arg1	slabs					560:564	dentine slabs	552:564	dentine slabs	552:564	Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva.
27223133	5	36	theme	treatments	663:672	arg1	treatments					663:672	the following treatments	649:672	the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose)	649:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	36	theme	treatments	663:672	arg1	starch					735:740	1% starch	732:740	1% starch	732:740	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	36	theme	treatments	663:672	arg1	one					642:644	one	642:644	one	642:644	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	36	theme	treatments	663:672	arg1	sucrose					720:726	10% sucrose	716:726	10% sucrose	716:726	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	36	theme	treatments	663:672	arg1	NaCl					680:683	0.9% NaCl	675:683	0.9% NaCl (negative control)	675:702	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	36	theme	treatments	663:672	arg1	sucrose					750:756	10% sucrose	746:756	10% sucrose (starch + sucrose)	746:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	36	theme	treatments	663:672	arg1	starch					708:713	1% starch	705:713	1% starch	705:713	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	2	37	theme	combination	280:290	arg1	cariogenicity					259:271	the cariogenicity	255:271	the cariogenicity of the combination of starch and sucrose	255:312	However, the cariogenicity of the combination of starch and sucrose remains unclear.
27223133	5	38	theme	%	733:733	arg1	starch					735:740	1% starch	732:740	1% starch	732:740	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	38	theme	%	733:733	arg1	treatments					663:672	the following treatments	649:672	the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose)	649:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	39	theme	%	706:706	arg1	treatments					663:672	the following treatments	649:672	the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose)	649:775	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	39	theme	%	706:706	arg1	starch					708:713	1% starch	705:713	1% starch	705:713	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	6	40	theme	amylase	819:825	arg1	effect					794:799	the effect	790:799	the effect of human salivary amylase on the starch metabolization	790:854	To simulate the effect of human salivary amylase on the starch metabolization, the biofilms were pretreated with saliva before each treatment and saliva was also added to the culture medium.
27223133	5	41	dep	sucrose	750:756	arg1	sucrose					768:774	sucrose	768:774	sucrose	768:774	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	5	41	dep	sucrose	750:756	arg1	starch					759:764	starch	759:764	starch	759:764	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	3	42	theme	biofilm	424:430	arg1	composition					432:442	Streptococcus mutans biofilm composition	403:442	Streptococcus mutans biofilm composition	403:442	The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization.
27223133	5	43	theme	10	746:747	arg1	%					748:748	%	748:748	%	748:748	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	6	44	theme	salivary	810:817	arg1	amylase					819:825	human salivary amylase	804:825	human salivary amylase	804:825	To simulate the effect of human salivary amylase on the starch metabolization, the biofilms were pretreated with saliva before each treatment and saliva was also added to the culture medium.
27223133	5	45	theme	0.9	675:677	arg1	%					678:678	%	678:678	%	678:678	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	7	46	theme	=	1123:1123	arg1	n					1121:1121	n = 9	1121:1125	n = 9	1121:1125	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	7	46	theme	=	1123:1123	arg1	biofilms					1111:1118	biofilms	1111:1118	biofilms (n = 9)	1111:1126	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	7	47	theme	growth	1103:1108	arg1	days					1095:1098	4 (dentine) or 5 (enamel) days	1069:1098	4 (dentine) or 5 (enamel) days of growth	1069:1108	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	7	48	theme	biofilm	990:996	arg1	Acidogenicity					969:981	Acidogenicity	969:981	Acidogenicity of the biofilm	969:996	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	4	49	theme	UA159	507:511	arg1	Biofilms					485:492	Biofilms	485:492	Biofilms of S. mutans UA159	485:511	Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva.
27223133	8	50	theme	loss	1345:1348	arg1	percentage					1314:1323	the percentage	1310:1323	the percentage of surface hardness loss	1310:1348	Dentine and enamel demineralization was assessed by determining the percentage of surface hardness loss.
27223133	3	51	dep	Streptococcus	403:415	arg1	mutans					417:422	mutans	417:422	mutans	417:422	The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization.
27223133	5	52	theme	10	716:717	arg1	%					718:718	%	718:718	%	718:718	They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose).
27223133	6	53	from	effect	794:799	arg1	metabolization					841:854	the starch metabolization	830:854	the starch metabolization	830:854	To simulate the effect of human salivary amylase on the starch metabolization, the biofilms were pretreated with saliva before each treatment and saliva was also added to the culture medium.
27223133	10	54	theme	cariogenic	1579:1588	arg1	potential					1590:1598	the cariogenic potential	1575:1598	the cariogenic potential of sucrose	1575:1609	The findings suggest that starch increases the cariogenic potential of sucrose.
27223133	7	55	theme	5	1084:1084	arg1	days					1095:1098	4 (dentine) or 5 (enamel) days	1069:1098	4 (dentine) or 5 (enamel) days of growth	1069:1108	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	4	56	theme	10	595:596	arg1	%					597:597	%	597:597	%	597:597	Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva.
27223133	7	57	dep	medium	1052:1057	arg1	pH.					1059:1061	the culture medium pH.	1040:1061	(2 times/day) the culture medium pH.	1026:1061	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	9	58	theme	higher	1420:1425	arg1	demineralization					1427:1442	higher demineralization	1420:1442	higher demineralization (p < 0.0001) on either enamel or dentine	1420:1483	Biofilms exposed to starch + sucrose were more acidogenic and caused higher demineralization (p < 0.0001) on either enamel or dentine than those exposed to each carbohydrate alone.
27223133	9	58	theme	higher	1420:1425	arg1	<					1447:1447	p < 0.0001	1445:1454	p < 0.0001	1445:1454	Biofilms exposed to starch + sucrose were more acidogenic and caused higher demineralization (p < 0.0001) on either enamel or dentine than those exposed to each carbohydrate alone.
27223133	3	59	theme	dentine	459:465	arg1	demineralization					467:482	dentine demineralization	459:482	dentine demineralization	459:482	The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization.
27223133	7	60	theme	4	1069:1069	arg1	days					1095:1098	4 (dentine) or 5 (enamel) days	1069:1098	4 (dentine) or 5 (enamel) days of growth	1069:1108	Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified.
27223133	4	61	contain	containing	584:593	arg2	saliva					599:604	10% saliva	595:604	10% saliva	595:604	Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva.
27223133	4	61	contain	containing	584:593	arg1	medium					577:582	culture medium	569:582	culture medium containing 10% saliva	569:604	Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva.
27223133	0	62	theme	starch	56:61	arg1	combination					41:51	a combination	39:51	a combination of starch and sucrose	39:73	Enamel and dentine demineralization by a combination of starch and sucrose in a biofilm - caries model.
27223133	0	63	from	demineralization	19:34	arg1	model					97:101	a biofilm - caries model	78:101	a biofilm - caries model	78:101	Enamel and dentine demineralization by a combination of starch and sucrose in a biofilm - caries model.
27223133	6	64	theme	culture	953:959	arg1	medium					961:966	the culture medium	949:966	the culture medium	949:966	To simulate the effect of human salivary amylase on the starch metabolization, the biofilms were pretreated with saliva before each treatment and saliva was also added to the culture medium.
27223133	6	65	theme	human	804:808	arg1	amylase					819:825	human salivary amylase	804:825	human salivary amylase	804:825	To simulate the effect of human salivary amylase on the starch metabolization, the biofilms were pretreated with saliva before each treatment and saliva was also added to the culture medium.
27223133	4	66	theme	%	597:597	arg1	saliva					599:604	10% saliva	595:604	10% saliva	595:604	Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva.
25843832	3	0	theme	water/ethanol	675:687	arg1	mixtures					689:696	water/ethanol mixtures	675:696	water/ethanol mixtures	675:696	Shape memory tests were carried out while the samples were immersed in varying compositions of water/ethanol mixtures.
25843832	1	1	from	applications	308:319	arg1	regeneration					329:340	bone regeneration	324:340	bone regeneration	324:340	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	7	2	theme	tissue	1317:1322	arg1	engineering					1324:1334	bone tissue engineering	1312:1334	bone tissue engineering	1312:1334	The results indicate that the developed CHT/BG-NPs nanocomposite scaffolds have potential for being applied in bone tissue engineering.
25843832	4	3	with	Dehydration	699:709	arg1	ethanol					716:722	ethanol	716:722	ethanol	716:722	Dehydration with ethanol enables to fix a temporary shape of a deformed scaffold that recovers the initial geometry upon water uptake.
25843832	3	4	theme	varying	651:657	arg1	compositions					659:670	varying compositions	651:670	varying compositions of water/ethanol mixtures	651:696	Shape memory tests were carried out while the samples were immersed in varying compositions of water/ethanol mixtures.
25843832	6	5	from	accommodation	1133:1145	arg1	defect					1193:1198	a bone defect	1186:1198	a bone defect	1186:1198	The applicability of such structures was demonstrated by a good geometrical accommodation of a previously compressed scaffold in a bone defect.
25843832	6	6	from	scaffold	1174:1181	arg1	defect					1193:1198	a bone defect	1186:1198	a bone defect	1186:1198	The applicability of such structures was demonstrated by a good geometrical accommodation of a previously compressed scaffold in a bone defect.
25843832	2	7	theme	bone-like	510:518	arg1	layer					528:532	bone-like apatite layer	510:532	bone-like apatite layer	510:532	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	5	8	theme	compressive	1031:1041	arg1	deformation					1043:1053	30% compressive deformation	1027:1053	30% compressive deformation	1027:1053	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	4	9	theme	temporary	741:749	arg1	shape					751:755	a temporary shape	739:755	a temporary shape of a deformed scaffold that recovers the initial geometry upon water uptake	739:831	Dehydration with ethanol enables to fix a temporary shape of a deformed scaffold that recovers the initial geometry upon water uptake.
25843832	1	10	theme	chitosan	251:258	arg1	ability					286:292	the biomineralization ability	264:292	the biomineralization ability of BG-NPs for applications in bone regeneration	264:340	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	1	10	theme	chitosan	251:258	arg1	properties					237:246	the shape memory properties	220:246	the shape memory properties of chitosan	220:258	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	4	11	theme	water	820:824	arg1	uptake					826:831	water uptake	820:831	water uptake	820:831	Dehydration with ethanol enables to fix a temporary shape of a deformed scaffold that recovers the initial geometry upon water uptake.
25843832	2	12	theme	CHT	401:403	arg1	matrix					415:420	the CHT polymeric matrix	397:420	the CHT polymeric matrix	397:420	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	3	13	theme	mixtures	689:696	arg1	compositions					659:670	varying compositions	651:670	varying compositions of water/ethanol mixtures	651:696	Shape memory tests were carried out while the samples were immersed in varying compositions of water/ethanol mixtures.
25843832	2	14	theme	scaffold	468:475	arg1	bioactivity					435:445	the bioactivity	431:445	the bioactivity of the nanocomposite scaffold	431:475	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	7	15	theme	developed	1231:1239	arg1	scaffolds					1266:1274	the developed CHT/BG-NPs nanocomposite scaffolds	1227:1274	the developed CHT/BG-NPs nanocomposite scaffolds	1227:1274	The results indicate that the developed CHT/BG-NPs nanocomposite scaffolds have potential for being applied in bone tissue engineering.
25843832	5	16	theme	%	944:944	arg1	ratio					974:978	a fixity ratio	965:978	a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation)	965:1054	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	5	16	theme	%	944:944	arg1	ratio					913:917	a recovery ratio	902:917	a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs	902:959	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	2	17	theme	sol-gel	380:386	arg1	route					388:392	a sol-gel route	378:392	a sol-gel route	378:392	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	0	18	theme	glass	19:23	arg1	nanoparticles					25:37	Chitosan/bioactive glass nanoparticles	0:37	Chitosan/bioactive glass nanoparticles	0:37	Chitosan/bioactive glass nanoparticles scaffolds with shape memory properties.
25843832	5	19	theme	%	1029:1029	arg1	deformation					1043:1053	30% compressive deformation	1027:1053	30% compressive deformation	1027:1053	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	6	20	theme	such	1078:1081	arg1	structures					1083:1092	such structures	1078:1092	such structures	1078:1092	The applicability of such structures was demonstrated by a good geometrical accommodation of a previously compressed scaffold in a bone defect.
25843832	7	21	theme	CHT/BG-NPs	1241:1250	arg1	scaffolds					1266:1274	the developed CHT/BG-NPs nanocomposite scaffolds	1227:1274	the developed CHT/BG-NPs nanocomposite scaffolds	1227:1274	The results indicate that the developed CHT/BG-NPs nanocomposite scaffolds have potential for being applied in bone tissue engineering.
25843832	0	22	theme	Chitosan/bioactive	0:17	arg1	nanoparticles					25:37	Chitosan/bioactive glass nanoparticles	0:37	Chitosan/bioactive glass nanoparticles	0:37	Chitosan/bioactive glass nanoparticles scaffolds with shape memory properties.
25843832	6	23	theme	good	1116:1119	arg1	accommodation					1133:1145	a good geometrical accommodation	1114:1145	a good geometrical accommodation of a previously compressed scaffold in a bone defect	1114:1198	The applicability of such structures was demonstrated by a good geometrical accommodation of a previously compressed scaffold in a bone defect.
25843832	6	24	theme	scaffold	1174:1181	arg1	accommodation					1133:1145	a good geometrical accommodation	1114:1145	a good geometrical accommodation of a previously compressed scaffold in a bone defect	1114:1198	The applicability of such structures was demonstrated by a good geometrical accommodation of a previously compressed scaffold in a bone defect.
25843832	6	25	theme	bone	1188:1191	arg1	defect					1193:1198	a bone defect	1186:1198	a bone defect	1186:1198	The applicability of such structures was demonstrated by a good geometrical accommodation of a previously compressed scaffold in a bone defect.
25843832	5	26	theme	good	856:859	arg1	properties					874:883	good shape memory properties	856:883	good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation)	856:1054	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	1	27	with	combination	92:102	arg1	nanoparticles					143:155	bioactive glass nanoparticles	127:155	bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration	127:340	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	1	27	with	combination	92:102	arg1	BG-NPs					158:163	BG-NPs	158:163	BG-NPs	158:163	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	1	28	theme	biomineralization	268:284	arg1	ability					286:292	the biomineralization ability	264:292	the biomineralization ability of BG-NPs for applications in bone regeneration	264:340	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	7	29	theme	bone	1312:1315	arg1	engineering					1324:1334	bone tissue engineering	1312:1334	bone tissue engineering	1312:1334	The results indicate that the developed CHT/BG-NPs nanocomposite scaffolds have potential for being applied in bone tissue engineering.
25843832	1	30	theme	chitosan	107:114	arg1	combination					92:102	a combination	90:102	a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration	90:340	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	6	31	theme	compressed	1163:1172	arg1	scaffold					1174:1181	a previously compressed scaffold	1150:1181	a previously compressed scaffold in a bone defect	1150:1198	The applicability of such structures was demonstrated by a good geometrical accommodation of a previously compressed scaffold in a bone defect.
25843832	2	32	theme	polymeric	405:413	arg1	matrix					415:420	the CHT polymeric matrix	397:420	the CHT polymeric matrix	397:420	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	1	33	theme	CHT/BG-NPs	186:195	arg1	scaffolds					197:205	CHT/BG-NPs scaffolds	186:205	CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration	186:340	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	0	34	theme	shape	54:58	arg1	properties					67:76	shape memory properties	54:76	shape memory properties	54:76	Chitosan/bioactive glass nanoparticles scaffolds with shape memory properties.
25843832	2	35	theme	layer	528:532	arg1	precipitation					493:505	the precipitation	489:505	the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF)	489:577	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	5	36	theme	%	1005:1005	arg1	ratio					974:978	a fixity ratio	965:978	a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation)	965:1054	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	5	36	theme	%	1005:1005	arg1	ratio					913:917	a recovery ratio	902:917	a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs	902:959	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	5	37	theme	fixity	967:972	arg1	ratio					974:978	a fixity ratio	965:978	a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation)	965:1054	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	7	38	theme	nanocomposite	1252:1264	arg1	scaffolds					1266:1274	the developed CHT/BG-NPs nanocomposite scaffolds	1227:1274	the developed CHT/BG-NPs nanocomposite scaffolds	1227:1274	The results indicate that the developed CHT/BG-NPs nanocomposite scaffolds have potential for being applied in bone tissue engineering.
25843832	2	39	theme	nanocomposite	454:466	arg1	scaffold					468:475	the nanocomposite scaffold	450:475	the nanocomposite scaffold	450:475	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	1	40	theme	BG-NPs	297:302	arg1	ability					286:292	the biomineralization ability	264:292	the biomineralization ability of BG-NPs for applications in bone regeneration	264:340	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	1	40	theme	BG-NPs	297:302	arg1	properties					237:246	the shape memory properties	220:246	the shape memory properties of chitosan	220:258	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	2	41	theme	apatite	520:526	arg1	layer					528:532	bone-like apatite layer	510:532	bone-like apatite layer	510:532	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	2	42	from	immersion	539:547	arg1	SBF					574:576	SBF	574:576	SBF	574:576	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	2	42	from	immersion	539:547	arg1	fluid					567:571	simulated body fluid	552:571	simulated body fluid (SBF)	552:577	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	5	43	theme	shape	861:865	arg1	properties					874:883	good shape memory properties	856:883	good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation)	856:1054	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	5	44	theme	30	1027:1028	arg1	%					1029:1029	%	1029:1029	%	1029:1029	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	0	45	theme	memory	60:65	arg1	properties					67:76	shape memory properties	54:76	shape memory properties	54:76	Chitosan/bioactive glass nanoparticles scaffolds with shape memory properties.
25843832	2	46	theme	body	562:565	arg1	SBF					574:576	SBF	574:576	SBF	574:576	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	2	46	theme	body	562:565	arg1	fluid					567:571	simulated body fluid	552:571	simulated body fluid (SBF)	552:577	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	5	47	theme	%	987:987	arg1	ratio					974:978	a fixity ratio	965:978	a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation)	965:1054	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	5	47	theme	%	987:987	arg1	ratio					913:917	a recovery ratio	902:917	a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs	902:959	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	4	48	theme	initial	798:804	arg1	geometry					806:813	the initial geometry	794:813	the initial geometry	794:813	Dehydration with ethanol enables to fix a temporary shape of a deformed scaffold that recovers the initial geometry upon water uptake.
25843832	4	49	theme	scaffold	771:778	arg1	shape					751:755	a temporary shape	739:755	a temporary shape of a deformed scaffold that recovers the initial geometry upon water uptake	739:831	Dehydration with ethanol enables to fix a temporary shape of a deformed scaffold that recovers the initial geometry upon water uptake.
25843832	2	50	theme	simulated	552:560	arg1	SBF					574:576	SBF	574:576	SBF	574:576	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	2	50	theme	simulated	552:560	arg1	fluid					567:571	simulated body fluid	552:571	simulated body fluid (SBF)	552:577	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	4	51	theme	deformed	762:769	arg1	scaffold					771:778	a deformed scaffold	760:778	a deformed scaffold that recovers the initial geometry upon water uptake	760:831	Dehydration with ethanol enables to fix a temporary shape of a deformed scaffold that recovers the initial geometry upon water uptake.
25843832	5	52	theme	recovery	904:911	arg1	ratio					913:917	a recovery ratio	902:917	a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs	902:959	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	5	53	theme	%	926:926	arg1	ratio					974:978	a fixity ratio	965:978	a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation)	965:1054	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	5	53	theme	%	926:926	arg1	ratio					913:917	a recovery ratio	902:917	a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs	902:959	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	3	54	dep	carried	604:610	arg1	out					612:614	out	612:614	out	612:614	Shape memory tests were carried out while the samples were immersed in varying compositions of water/ethanol mixtures.
25843832	6	55	theme	structures	1083:1092	arg1	applicability					1061:1073	The applicability	1057:1073	The applicability of such structures	1057:1092	The applicability of such structures was demonstrated by a good geometrical accommodation of a previously compressed scaffold in a bone defect.
25843832	7	56	contain	have	1276:1279	arg2	potential					1281:1289	potential	1281:1289	potential for being applied in bone tissue engineering	1281:1334	The results indicate that the developed CHT/BG-NPs nanocomposite scaffolds have potential for being applied in bone tissue engineering.
25843832	7	56	contain	have	1276:1279	arg1	scaffolds					1266:1274	the developed CHT/BG-NPs nanocomposite scaffolds	1227:1274	the developed CHT/BG-NPs nanocomposite scaffolds	1227:1274	The results indicate that the developed CHT/BG-NPs nanocomposite scaffolds have potential for being applied in bone tissue engineering.
25843832	6	57	theme	geometrical	1121:1131	arg1	accommodation					1133:1145	a good geometrical accommodation	1114:1145	a good geometrical accommodation of a previously compressed scaffold in a bone defect	1114:1198	The applicability of such structures was demonstrated by a good geometrical accommodation of a previously compressed scaffold in a bone defect.
25843832	1	58	theme	bioactive	127:135	arg1	nanoparticles					143:155	bioactive glass nanoparticles	127:155	bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration	127:340	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	1	58	theme	bioactive	127:135	arg1	BG-NPs					158:163	BG-NPs	158:163	BG-NPs	158:163	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	6	59	from	defect	1193:1198	arg1	accommodation					1133:1145	a good geometrical accommodation	1114:1145	a good geometrical accommodation of a previously compressed scaffold in a bone defect	1114:1198	The applicability of such structures was demonstrated by a good geometrical accommodation of a previously compressed scaffold in a bone defect.
25843832	5	60	theme	memory	867:872	arg1	properties					874:883	good shape memory properties	856:883	good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation)	856:1054	The scaffolds present good shape memory properties characterized by a recovery ratio of 87.5% for CHT and 89.9% for CHT/BG-NPs and a fixity ratio of 97.2% for CHT and 98.2% for CHT/BG-NPs (for 30% compressive deformation).
25843832	3	61	theme	Shape	580:584	arg1	tests					593:597	Shape memory tests	580:597	Shape memory tests	580:597	Shape memory tests were carried out while the samples were immersed in varying compositions of water/ethanol mixtures.
25843832	1	62	theme	glass	137:141	arg1	nanoparticles					143:155	bioactive glass nanoparticles	127:155	bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration	127:340	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	1	62	theme	glass	137:141	arg1	BG-NPs					158:163	BG-NPs	158:163	BG-NPs	158:163	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	1	63	theme	shape	224:228	arg1	properties					237:246	the shape memory properties	220:246	the shape memory properties of chitosan	220:258	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	1	64	theme	bone	324:327	arg1	regeneration					329:340	bone regeneration	324:340	bone regeneration	324:340	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25843832	3	65	theme	memory	586:591	arg1	tests					593:597	Shape memory tests	580:597	Shape memory tests	580:597	Shape memory tests were carried out while the samples were immersed in varying compositions of water/ethanol mixtures.
25843832	2	66	theme	BG-NPs	359:364	arg1	addition					347:354	The addition	343:354	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix	343:420	The addition of BG-NPs prepared by a sol-gel route to the CHT polymeric matrix improved the bioactivity of the nanocomposite scaffold, as seen by the precipitation of bone-like apatite layer upon immersion in simulated body fluid (SBF).
25843832	1	67	theme	memory	230:235	arg1	properties					237:246	the shape memory properties	220:246	the shape memory properties of chitosan	220:258	We propose a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce CHT/BG-NPs scaffolds that combine the shape memory properties of chitosan and the biomineralization ability of BG-NPs for applications in bone regeneration.
25009978	7	0	theme	platforms	1479:1487	arg1	development					1444:1454	the development	1440:1454	the development of novel gene delivery platforms	1440:1487	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	5	1	from	compositions	914:925	arg1	~300-350nm					1060:1069	~300-350nm	1060:1069	~300-350nm	1060:1069	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	1	2	theme	gene	172:175	arg1	expression					177:186	sustained gene expression	162:186	sustained gene expression	162:186	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	5	3	theme	similar	1120:1126	arg1	transfection					1107:1118	efficient transfection	1097:1118	efficient transfection similar to amphotropic MLV vectors	1097:1153	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	3	4	theme	agents	538:543	arg1	design					507:512	The design	503:512	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors)	503:616	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	1	5	theme	poor	274:277	arg1	stability					279:287	poor stability	274:287	poor stability	274:287	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	5	6	theme	chitosan/10	1001:1011	arg1	M-VLPs					1016:1021	40μg chitosan/10(9) M-VLPs	996:1021	40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml	996:1043	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	2	7	from	contrast	345:352	arg1	robust					386:391	robust	386:391	robust	386:391	In contrast, polymer-based vectors are more robust and allow cell- and tissue-specific deliveries via conjugation of ligands, but are comparatively inefficient.
25009978	6	8	from	non-cytotoxic	1195:1207	arg1	addition					1159:1166	addition	1159:1166	addition	1159:1166	In addition, these nanobiovectors were non-cytotoxic and provided sustained transgene expression for at least three weeks in vitro.
25009978	3	9	link	derived	569:575	arg1	nanobiovectors					602:615	nanobiovectors	602:615	nanobiovectors	602:615	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	3	9	link	derived	569:575	arg1	materials					591:599	both virally derived and synthetic materials	556:599	both virally derived and synthetic materials (nanobiovectors)	556:616	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	7	10	theme	gene	1465:1468	arg1	platforms					1479:1487	novel gene delivery platforms	1459:1487	novel gene delivery platforms	1459:1487	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	3	11	theme	synthetic	581:589	arg1	nanobiovectors					602:615	nanobiovectors	602:615	nanobiovectors	602:615	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	3	11	theme	synthetic	581:589	arg1	materials					591:599	both virally derived and synthetic materials	556:599	both virally derived and synthetic materials (nanobiovectors)	556:616	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	3	12	theme	delivery	529:536	arg1	agents					538:543	hybrid gene delivery agents	517:543	hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors)	517:616	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	6	13	from	addition	1159:1166	arg1	non-cytotoxic					1195:1207	non-cytotoxic	1195:1207	non-cytotoxic	1195:1207	In addition, these nanobiovectors were non-cytotoxic and provided sustained transgene expression for at least three weeks in vitro.
25009978	7	14	theme	delivery	1470:1477	arg1	platforms					1479:1487	novel gene delivery platforms	1459:1487	novel gene delivery platforms	1459:1487	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	7	15	theme	hybrid	1396:1401	arg1	vector					1403:1408	a highly efficient hybrid vector	1377:1408	a highly efficient hybrid vector	1377:1408	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	7	16	theme	biocompatible	1308:1320	arg1	agents					1332:1337	biocompatible synthetic agents	1308:1337	biocompatible synthetic agents	1308:1337	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	1	17	theme	Recombinant	76:86	arg1	retroviruses					88:99	Recombinant retroviruses	76:99	Recombinant retroviruses	76:99	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	4	18	theme	murine	724:729	arg1	particles					751:759	Non-infectious murine leukemia virus-like particles	709:759	Non-infectious murine leukemia virus-like particles (M-VLPs)	709:768	Non-infectious murine leukemia virus-like particles (M-VLPs) were electrostatically complexed with chitosan (χ) to replace the function of the viral envelope protein.
25009978	4	18	theme	murine	724:729	arg1	M-VLPs					762:767	M-VLPs	762:767	M-VLPs	762:767	Non-infectious murine leukemia virus-like particles (M-VLPs) were electrostatically complexed with chitosan (χ) to replace the function of the viral envelope protein.
25009978	2	19	theme	tissue-specific	413:427	arg1	deliveries					429:438	cell- and tissue-specific deliveries	403:438	cell- and tissue-specific deliveries	403:438	In contrast, polymer-based vectors are more robust and allow cell- and tissue-specific deliveries via conjugation of ligands, but are comparatively inefficient.
25009978	3	20	theme	derived	569:575	arg1	nanobiovectors					602:615	nanobiovectors	602:615	nanobiovectors	602:615	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	3	20	theme	derived	569:575	arg1	materials					591:599	both virally derived and synthetic materials	556:599	both virally derived and synthetic materials (nanobiovectors)	556:616	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	7	21	theme	inactive	1344:1351	arg1	particles					1359:1367	inactive viral particles	1344:1367	inactive viral particles to form a highly efficient hybrid vector	1344:1408	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	0	22	theme	in	10:11	arg1	delivery					24:31	Efficient in vitro gene delivery	0:31	Efficient in vitro gene delivery by hybrid biopolymer/virus	0:58	Efficient in vitro gene delivery by hybrid biopolymer/virus nanobiovectors.
25009978	7	23	theme	novel	1459:1463	arg1	platforms					1479:1487	novel gene delivery platforms	1459:1487	novel gene delivery platforms	1459:1487	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	2	24	theme	cell-	403:407	arg1	deliveries					429:438	cell- and tissue-specific deliveries	403:438	cell- and tissue-specific deliveries	403:438	In contrast, polymer-based vectors are more robust and allow cell- and tissue-specific deliveries via conjugation of ligands, but are comparatively inefficient.
25009978	0	25	theme	Efficient	0:8	arg1	delivery					24:31	Efficient in vitro gene delivery	0:31	Efficient in vitro gene delivery by hybrid biopolymer/virus	0:58	Efficient in vitro gene delivery by hybrid biopolymer/virus nanobiovectors.
25009978	1	26	theme	limited	293:299	arg1	flexibility					301:311	limited flexibility	293:311	limited flexibility for modification of tropism	293:339	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	5	27	dep	9μg	946:948	arg1	to					943:944	to	943:944	to	943:944	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	4	28	theme	Non-infectious	709:722	arg1	particles					751:759	Non-infectious murine leukemia virus-like particles	709:759	Non-infectious murine leukemia virus-like particles (M-VLPs)	709:768	Non-infectious murine leukemia virus-like particles (M-VLPs) were electrostatically complexed with chitosan (χ) to replace the function of the viral envelope protein.
25009978	4	28	theme	Non-infectious	709:722	arg1	M-VLPs					762:767	M-VLPs	762:767	M-VLPs	762:767	Non-infectious murine leukemia virus-like particles (M-VLPs) were electrostatically complexed with chitosan (χ) to replace the function of the viral envelope protein.
25009978	0	29	theme	gene	19:22	arg1	delivery					24:31	Efficient in vitro gene delivery	0:31	Efficient in vitro gene delivery by hybrid biopolymer/virus	0:58	Efficient in vitro gene delivery by hybrid biopolymer/virus nanobiovectors.
25009978	7	30	theme	significant	1415:1425	arg1	combination					1293:1303	This combination	1288:1303	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector	1288:1408	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	7	30	theme	significant	1415:1425	arg1	extension					1427:1435	a significant extension	1413:1435	a significant extension in the development of novel gene delivery platforms	1413:1487	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	2	31	from	robust	386:391	arg1	contrast					345:352	contrast	345:352	contrast	345:352	In contrast, polymer-based vectors are more robust and allow cell- and tissue-specific deliveries via conjugation of ligands, but are comparatively inefficient.
25009978	1	32	theme	efficient	116:124	arg1	delivery					131:138	highly efficient gene delivery	109:138	highly efficient gene delivery	109:138	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	4	33	theme	envelope	858:865	arg1	protein					867:873	the viral envelope protein	848:873	the viral envelope protein	848:873	Non-infectious murine leukemia virus-like particles (M-VLPs) were electrostatically complexed with chitosan (χ) to replace the function of the viral envelope protein.
25009978	1	34	theme	significant	205:215	arg1	disadvantages					217:229	significant disadvantages	205:229	significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism	205:339	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	1	34	theme	significant	205:215	arg1	titer					245:249	low titer	241:249	low titer	241:249	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	1	34	theme	significant	205:215	arg1	production					262:271	expensive production	252:271	expensive production	252:271	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	1	34	theme	significant	205:215	arg1	stability					279:287	poor stability	274:287	poor stability	274:287	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	1	34	theme	significant	205:215	arg1	flexibility					301:311	limited flexibility	293:311	limited flexibility for modification of tropism	293:339	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	2	35	theme	ligands	459:465	arg1	conjugation					444:454	conjugation	444:454	conjugation of ligands	444:465	In contrast, polymer-based vectors are more robust and allow cell- and tissue-specific deliveries via conjugation of ligands, but are comparatively inefficient.
25009978	7	36	with	combination	1293:1303	arg1	particles					1359:1367	inactive viral particles	1344:1367	inactive viral particles to form a highly efficient hybrid vector	1344:1408	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	3	37	theme	gene	687:690	arg1	vectors					700:706	safe and efficient gene therapy vectors	668:706	safe and efficient gene therapy vectors	668:706	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	1	38	theme	gene	126:129	arg1	delivery					131:138	highly efficient gene delivery	109:138	highly efficient gene delivery	109:138	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	5	39	theme	optimal	879:885	arg1	conditions					899:908	optimal fabrication conditions	879:908	optimal fabrication conditions	879:908	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	3	40	theme	gene	524:527	arg1	agents					538:543	hybrid gene delivery agents	517:543	hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors)	517:616	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	3	41	theme	therapy	692:698	arg1	vectors					700:706	safe and efficient gene therapy vectors	668:706	safe and efficient gene therapy vectors	668:706	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	3	42	theme	promising	631:639	arg1	approach					641:648	a promising approach	629:648	a promising approach to development of safe and efficient gene therapy vectors	629:706	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	7	43	theme	agents	1332:1337	arg1	combination					1293:1303	This combination	1288:1303	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector	1288:1408	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	7	43	theme	agents	1332:1337	arg1	extension					1427:1435	a significant extension	1413:1435	a significant extension in the development of novel gene delivery platforms	1413:1487	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	5	44	theme	fabrication	887:897	arg1	conditions					899:908	optimal fabrication conditions	879:908	optimal fabrication conditions	879:908	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	5	45	theme	amphotropic	1131:1141	arg1	vectors					1147:1153	amphotropic MLV vectors	1131:1153	amphotropic MLV vectors	1131:1153	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	7	46	theme	synthetic	1322:1330	arg1	agents					1332:1337	biocompatible synthetic agents	1308:1337	biocompatible synthetic agents	1308:1337	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	4	47	theme	viral	852:856	arg1	protein					867:873	the viral envelope protein	848:873	the viral envelope protein	848:873	Non-infectious murine leukemia virus-like particles (M-VLPs) were electrostatically complexed with chitosan (χ) to replace the function of the viral envelope protein.
25009978	1	48	theme	low	241:243	arg1	titer					245:249	low titer	241:249	low titer	241:249	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	1	49	theme	tropism	333:339	arg1	modification					317:328	modification	317:328	modification of tropism	317:339	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	7	50	theme	viral	1353:1357	arg1	particles					1359:1367	inactive viral particles	1344:1367	inactive viral particles to form a highly efficient hybrid vector	1344:1408	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	3	51	theme	efficient	677:685	arg1	vectors					700:706	safe and efficient gene therapy vectors	668:706	safe and efficient gene therapy vectors	668:706	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	6	52	theme	sustained	1222:1230	arg1	expression					1242:1251	sustained transgene expression	1222:1251	sustained transgene expression	1222:1251	In addition, these nanobiovectors were non-cytotoxic and provided sustained transgene expression for at least three weeks in vitro.
25009978	5	53	from	conditions	899:908	arg1	~300-350nm					1060:1069	~300-350nm	1060:1069	~300-350nm	1060:1069	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	3	54	theme	hybrid	517:522	arg1	agents					538:543	hybrid gene delivery agents	517:543	hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors)	517:616	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	0	55	theme	hybrid	36:41	arg1	biopolymer/virus					43:58	hybrid biopolymer/virus	36:58	hybrid biopolymer/virus	36:58	Efficient in vitro gene delivery by hybrid biopolymer/virus nanobiovectors.
25009978	7	56	theme	efficient	1386:1394	arg1	vector					1403:1408	a highly efficient hybrid vector	1377:1408	a highly efficient hybrid vector	1377:1408	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	4	57	theme	protein	867:873	arg1	function					836:843	the function	832:843	the function of the viral envelope protein	832:873	Non-infectious murine leukemia virus-like particles (M-VLPs) were electrostatically complexed with chitosan (χ) to replace the function of the viral envelope protein.
25009978	2	58	theme	polymer-based	355:367	arg1	vectors					369:375	polymer-based vectors	355:375	polymer-based vectors	355:375	In contrast, polymer-based vectors are more robust and allow cell- and tissue-specific deliveries via conjugation of ligands, but are comparatively inefficient.
25009978	5	59	theme	40μg	996:999	arg1	M-VLPs					1016:1021	40μg chitosan/10(9) M-VLPs	996:1021	40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml	996:1043	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	6	60	theme	transgene	1232:1240	arg1	expression					1242:1251	sustained transgene expression	1222:1251	sustained transgene expression	1222:1251	In addition, these nanobiovectors were non-cytotoxic and provided sustained transgene expression for at least three weeks in vitro.
25009978	3	61	theme	vectors	700:706	arg1	development					653:663	development	653:663	development of safe and efficient gene therapy vectors	653:706	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	3	62	theme	safe	668:671	arg1	vectors					700:706	safe and efficient gene therapy vectors	668:706	safe and efficient gene therapy vectors	668:706	The design of hybrid gene delivery agents comprising both virally derived and synthetic materials (nanobiovectors) represents a promising approach to development of safe and efficient gene therapy vectors.
25009978	7	63	dep	particles	1359:1367	arg1	form					1372:1375	form	1372:1375	to form a highly efficient hybrid vector	1369:1408	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	4	64	theme	leukemia	731:738	arg1	particles					751:759	Non-infectious murine leukemia virus-like particles	709:759	Non-infectious murine leukemia virus-like particles (M-VLPs)	709:768	Non-infectious murine leukemia virus-like particles (M-VLPs) were electrostatically complexed with chitosan (χ) to replace the function of the viral envelope protein.
25009978	4	64	theme	leukemia	731:738	arg1	M-VLPs					762:767	M-VLPs	762:767	M-VLPs	762:767	Non-infectious murine leukemia virus-like particles (M-VLPs) were electrostatically complexed with chitosan (χ) to replace the function of the viral envelope protein.
25009978	5	65	from	~300-350nm	1060:1069	arg1	diameter					1074:1081	diameter	1074:1081	diameter	1074:1081	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	0	66	dep	in	10:11	arg1	vitro					13:17	vitro	13:17	vitro	13:17	Efficient in vitro gene delivery by hybrid biopolymer/virus nanobiovectors.
25009978	4	67	theme	virus-like	740:749	arg1	particles					751:759	Non-infectious murine leukemia virus-like particles	709:759	Non-infectious murine leukemia virus-like particles (M-VLPs)	709:768	Non-infectious murine leukemia virus-like particles (M-VLPs) were electrostatically complexed with chitosan (χ) to replace the function of the viral envelope protein.
25009978	4	67	theme	virus-like	740:749	arg1	M-VLPs					762:767	M-VLPs	762:767	M-VLPs	762:767	Non-infectious murine leukemia virus-like particles (M-VLPs) were electrostatically complexed with chitosan (χ) to replace the function of the viral envelope protein.
25009978	1	68	theme	expensive	252:260	arg1	production					262:271	expensive production	252:271	expensive production	252:271	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	5	69	from	M-VLPs/ml	1035:1043	arg1	M-VLPs					1016:1021	40μg chitosan/10(9) M-VLPs	996:1021	40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml	996:1043	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	5	70	theme	efficient	1097:1105	arg1	transfection					1107:1118	efficient transfection	1097:1118	efficient transfection similar to amphotropic MLV vectors	1097:1153	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	5	71	from	diameter	1074:1081	arg1	~300-350nm					1060:1069	~300-350nm	1060:1069	~300-350nm	1060:1069	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
25009978	1	72	theme	sustained	162:170	arg1	expression					177:186	sustained gene expression	162:186	sustained gene expression	162:186	Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
25009978	7	73	from	extension	1427:1435	arg1	development					1444:1454	the development	1440:1454	the development of novel gene delivery platforms	1440:1487	This combination of biocompatible synthetic agents with inactive viral particles to form a highly efficient hybrid vector is a significant extension in the development of novel gene delivery platforms.
25009978	5	74	theme	MLV	1143:1145	arg1	vectors					1147:1153	amphotropic MLV vectors	1131:1153	amphotropic MLV vectors	1131:1153	At optimal fabrication conditions and compositions, ranging from 6 to 9μg chitosan/10(9) M-VLPs at 10×10(9)M-VLPs/ml to 40μg chitosan/10(9) M-VLPs at 2.5×10(9)M-VLPs/ml, χ/M-VLPs were ~300-350nm in diameter and exhibited efficient transfection similar to amphotropic MLV vectors.
26395628	0	0	theme	Tissue	72:77	arg1	Engineering					79:89	Human Skin Tissue Engineering	61:89	Human Skin Tissue Engineering	61:89	Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering.
26395628	4	1	theme	mechanical	947:956	arg1	properties					958:967	mechanical properties	947:967	mechanical properties of the collagen scaffolds	947:993	However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds.
26395628	6	2	theme	skin	1293:1296	arg1	wounds					1298:1303	skin wounds	1293:1303	skin wounds	1293:1303	Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective.
26395628	1	3	theme	scaffolds	147:155	arg1	structure					108:116	structure	108:116	structure	108:116	We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers.
26395628	1	3	theme	scaffolds	147:155	arg1	properties					133:142	mechanical properties	122:142	mechanical properties	122:142	We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers.
26395628	1	4	theme	polymers	219:226	arg1	collagen					171:178	pure collagen	166:178	pure collagen	166:178	We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers.
26395628	1	4	theme	polymers	219:226	arg1	chitosan					186:193	pure chitosan	181:193	pure chitosan	181:193	We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers.
26395628	1	4	theme	polymers	219:226	arg1	mixture					202:208	a mixture	200:208	a mixture of these polymers	200:226	We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers.
26395628	0	5	theme	Skin	67:70	arg1	Engineering					79:89	Human Skin Tissue Engineering	61:89	Human Skin Tissue Engineering	61:89	Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering.
26395628	4	6	theme	chitosan	817:824	arg1	use					810:812	the use	806:812	the use of chitosan as an additive	806:839	However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds.
26395628	2	7	theme	functions	311:319	arg1	maintenance					291:301	the maintenance	287:301	the maintenance of cell functions (proliferation, differentiation, and migration)	287:367	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	8	theme	skin	491:494	arg1	equivalents					496:506	complex skin equivalents	483:506	complex skin equivalents (fibroblasts and keratinocytes)	483:538	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	5	9	from	resistance	1123:1132	arg1	comparison					1185:1194	comparison	1185:1194	comparison with glutaraldehyde treatment	1185:1224	The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment.
26395628	7	10	theme	chitosan	1428:1435	arg1	impractical					1503:1513	impractical	1503:1513	impractical	1503:1513	We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.
26395628	7	10	theme	chitosan	1428:1435	arg1	use					1421:1423	the use	1417:1423	the use of chitosan as the scaffold for skin equivalents populated with skin cells	1417:1498	We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.
26395628	3	11	theme	extracellular	775:787	arg1	matrix					789:794	natural extracellular matrix	767:794	natural extracellular matrix	767:794	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	2	12	theme	complex	483:489	arg1	equivalents					496:506	complex skin equivalents	483:506	complex skin equivalents (fibroblasts and keratinocytes)	483:538	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	13	dep	analogues	433:441	arg1	populated					453:461	populated	453:461	populated by fibroblasts	453:476	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	6	14	theme	complete	1273:1280	arg1	healing					1282:1288	complete healing	1273:1288	complete healing of skin wounds	1273:1303	Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective.
26395628	2	15	theme	composition	245:255	arg1	role					233:236	The role	229:236	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration)	229:367	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	7	16	theme	effective	1537:1545	arg1	modifier					1547:1554	an effective modifier	1534:1554	an effective modifier of polymer scaffolds	1534:1575	We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.
26395628	7	16	theme	effective	1537:1545	arg1	it					1524:1525	it	1524:1525	it	1524:1525	We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.
26395628	6	17	theme	pure	1356:1359	arg1	sponge					1370:1375	pure chitosan sponge	1356:1375	pure chitosan sponge	1356:1375	Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective.
26395628	3	18	theme	specific	686:693	arg1	spheroids					719:727	spheroids	719:727	spheroids	719:727	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	3	18	theme	specific	686:693	arg1	conglomerates					704:716	specific cellular conglomerates	686:716	specific cellular conglomerates	686:716	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	0	19	theme	Artificial	37:46	arg1	Scaffold					48:55	Artificial Scaffold	37:55	Artificial Scaffold for Human Skin Tissue Engineering	37:89	Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering.
26395628	1	20	dep	structure	108:116	arg1	the					104:106	the	104:106	the	104:106	We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers.
26395628	1	21	theme	pure	166:169	arg1	collagen					171:178	pure collagen	166:178	pure collagen	166:178	We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers.
26395628	2	22	dep	composition	245:255	arg1	the					241:243	the	241:243	the	241:243	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	7	23	theme	skin	1457:1460	arg1	equivalents					1462:1472	skin equivalents	1457:1472	skin equivalents populated with skin cells	1457:1498	We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.
26395628	4	24	theme	scaffolds	985:993	arg1	properties					958:967	mechanical properties	947:967	mechanical properties of the collagen scaffolds	947:993	However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds.
26395628	2	25	dep	models	406:411	arg1	equivalents					496:506	complex skin equivalents	483:506	complex skin equivalents (fibroblasts and keratinocytes)	483:538	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	25	dep	models	406:411	arg1	analogues					433:441	homogeneous tissue analogues	414:441	homogeneous tissue analogues (scaffold populated by fibroblasts)	414:477	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	4	26	theme	collagen	976:983	arg1	scaffolds					985:993	the collagen scaffolds	972:993	the collagen scaffolds	972:993	However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds.
26395628	2	27	theme	scaffolds	274:282	arg1	composition					245:255	composition	245:255	composition	245:255	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	27	theme	scaffolds	274:282	arg1	structure					261:269	structure	261:269	structure	261:269	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	6	28	theme	composition	1335:1345	arg1	healing					1347:1353	their composition healing	1329:1353	their composition healing	1329:1353	Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective.
26395628	3	29	dep	scaffolds	565:573	arg1	contrast					544:551	contrast	544:551	contrast	544:551	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	2	30	theme	experimental	393:404	arg1	models					406:411	two experimental models	389:411	two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes)	389:538	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	5	31	theme	fibroblast	1159:1168	arg1	contraction					1170:1180	fibroblast contraction	1159:1180	fibroblast contraction	1159:1180	The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment.
26395628	1	32	theme	pure	181:184	arg1	chitosan					186:193	pure chitosan	181:193	pure chitosan	181:193	We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers.
26395628	3	33	theme	cellular	695:702	arg1	spheroids					719:727	spheroids	719:727	spheroids	719:727	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	3	33	theme	cellular	695:702	arg1	conglomerates					704:716	specific cellular conglomerates	686:716	specific cellular conglomerates	686:716	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	4	34	theme	fibroblasts	878:888	arg1	activity					866:873	proliferative activity	852:873	proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds	852:993	However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds.
26395628	5	35	theme	additional	1032:1041	arg1	agent					1057:1061	an additional cross-linking agent	1029:1061	an additional cross-linking agent	1029:1061	The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment.
26395628	2	36	theme	structure	261:269	arg1	role					233:236	The role	229:236	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration)	229:367	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	37	from	role	233:236	arg1	maintenance					291:301	the maintenance	287:301	the maintenance of cell functions (proliferation, differentiation, and migration)	287:367	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	5	38	with	comparison	1185:1194	arg1	treatment					1216:1224	glutaraldehyde treatment	1201:1224	glutaraldehyde treatment	1201:1224	The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment.
26395628	3	39	theme	fibroblasts	614:624	arg1	growth					604:609	the growth	600:609	the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids	600:727	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	3	40	theme	collagen	556:563	arg1	scaffolds					565:573	collagen scaffolds	556:573	collagen scaffolds	556:573	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	7	41	theme	skin	1489:1492	arg1	cells					1494:1498	skin cells	1489:1498	skin cells	1489:1498	We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.
26395628	4	42	theme	properties	958:967	arg1	improvement					932:942	improvement	932:942	improvement of mechanical properties of the collagen scaffolds	932:993	However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds.
26395628	0	43	theme	Modifying	14:22	arg1	Component					24:32	a Modifying Component	12:32	a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering	12:89	Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering.
26395628	0	44	theme	Scaffold	48:55	arg1	Component					24:32	a Modifying Component	12:32	a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering	12:89	Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering.
26395628	4	45	theme	proliferative	852:864	arg1	activity					866:873	proliferative activity	852:873	proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds	852:993	However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds.
26395628	6	46	theme	Polymer	1227:1233	arg1	scaffolds					1235:1243	Polymer scaffolds	1227:1243	Polymer scaffolds (without cells)	1227:1259	Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective.
26395628	2	47	theme	cell	306:309	arg1	differentiation					337:351	differentiation	337:351	differentiation	337:351	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	47	theme	cell	306:309	arg1	migration					358:366	migration	358:366	migration	358:366	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	47	theme	cell	306:309	arg1	proliferation					322:334	proliferation	322:334	proliferation	322:334	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	47	theme	cell	306:309	arg1	functions					311:319	cell functions	306:319	cell functions (proliferation, differentiation, and migration)	306:367	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	48	theme	homogeneous	414:424	arg1	analogues					433:441	homogeneous tissue analogues	414:441	homogeneous tissue analogues (scaffold populated by fibroblasts)	414:477	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	5	49	theme	collagen	1137:1144	arg1	scaffolds					1146:1154	collagen scaffolds	1137:1154	collagen scaffolds	1137:1154	The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment.
26395628	5	50	theme	chitosan	1017:1024	arg1	effectiveness					1000:1012	The effectiveness	996:1012	The effectiveness of chitosan as an additional cross-linking agent	996:1061	The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment.
26395628	2	51	dep	functions	311:319	arg1	differentiation					337:351	differentiation	337:351	differentiation	337:351	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	51	dep	functions	311:319	arg1	migration					358:366	migration	358:366	migration	358:366	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	51	dep	functions	311:319	arg1	proliferation					322:334	proliferation	322:334	proliferation	322:334	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	51	dep	functions	311:319	arg1	functions					311:319	cell functions	306:319	cell functions (proliferation, differentiation, and migration)	306:367	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	5	52	theme	scaffolds	1146:1154	arg1	resistance					1123:1132	the resistance	1119:1132	the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment	1119:1224	The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment.
26395628	4	53	from	activity	866:873	arg1	collagen					893:900	collagen	893:900	collagen	893:900	However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds.
26395628	5	54	theme	glutaraldehyde	1201:1214	arg1	treatment					1216:1224	glutaraldehyde treatment	1201:1224	glutaraldehyde treatment	1201:1224	The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment.
26395628	6	55	theme	wounds	1298:1303	arg1	healing					1282:1288	complete healing	1273:1288	complete healing of skin wounds	1273:1303	Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective.
26395628	5	56	theme	cross-linking	1043:1055	arg1	agent					1057:1061	an additional cross-linking agent	1029:1061	an additional cross-linking agent	1029:1061	The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment.
26395628	1	57	theme	mechanical	122:131	arg1	properties					133:142	mechanical properties	122:142	mechanical properties	122:142	We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers.
26395628	6	58	theme	chitosan	1361:1368	arg1	sponge					1370:1375	pure chitosan sponge	1356:1375	pure chitosan sponge	1356:1375	Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective.
26395628	3	59	theme	natural	767:773	arg1	matrix					789:794	natural extracellular matrix	767:794	natural extracellular matrix	767:794	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	0	60	theme	Human	61:65	arg1	Engineering					79:89	Human Skin Tissue Engineering	61:89	Human Skin Tissue Engineering	61:89	Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering.
26395628	7	61	theme	scaffolds	1567:1575	arg1	modifier					1547:1554	an effective modifier	1534:1554	an effective modifier of polymer scaffolds	1534:1575	We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.
26395628	7	61	theme	scaffolds	1567:1575	arg1	it					1524:1525	it	1524:1525	it	1524:1525	We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.
26395628	7	62	theme	polymer	1559:1565	arg1	scaffolds					1567:1575	polymer scaffolds	1559:1575	polymer scaffolds	1559:1575	We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.
26395628	2	63	theme	tissue	426:431	arg1	analogues					433:441	homogeneous tissue analogues	414:441	homogeneous tissue analogues (scaffold populated by fibroblasts)	414:477	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	3	64	theme	chitosan	658:665	arg1	fibers					667:672	chitosan fibers	658:672	chitosan fibers	658:672	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	3	65	theme	pure	576:579	arg1	chitosan					581:588	pure chitosan	576:588	pure chitosan	576:588	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	3	66	with	contacts	644:651	arg1	fibers					667:672	chitosan fibers	658:672	chitosan fibers	658:672	In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix.
26395628	2	67	dep	equivalents	496:506	arg1	fibroblasts					509:519	fibroblasts	509:519	fibroblasts	509:519	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
26395628	2	67	dep	equivalents	496:506	arg1	keratinocytes					525:537	keratinocytes	525:537	keratinocytes	525:537	The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes).
28482654	0	0	theme	Nanofibrils	111:121	arg1	Integration					86:96	Nanocomposite Integration	72:96	Nanocomposite Integration of Cellulose Nanofibrils	72:121	Surface-Initiated Controlled Radical Polymerization Approach To Enhance Nanocomposite Integration of Cellulose Nanofibrils.
28482654	2	1	theme	stearyl	537:543	arg1	acrylate					545:552	stearyl acrylate	537:552	stearyl acrylate	537:552	For that purpose, CNF was converted to a CNF-based microinitiator through an esterification reaction on the nanofibril surfaces, which efficiently initiated the controlled radical grafting polymerization of stearyl acrylate.
28482654	5	2	theme	electron	866:873	arg1	microscopy					875:884	Scanning electron microscopy	857:884	Scanning electron microscopy	857:884	Scanning electron microscopy reveals a homogeneous dispersion of the hydrophobic CNF in composite matrix with no signs of aggregation.
28482654	1	3	theme	cellulose	180:188	arg1	CNFs					203:206	CNFs	203:206	CNFs	203:206	A strategy is devised for the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix.
28482654	1	3	theme	cellulose	180:188	arg1	nanofibrils					190:200	hydrophilic cellulose nanofibrils	168:200	hydrophilic cellulose nanofibrils (CNFs)	168:207	A strategy is devised for the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix.
28482654	0	4	theme	Cellulose	101:109	arg1	Nanofibrils					111:121	Cellulose Nanofibrils	101:121	Cellulose Nanofibrils	101:121	Surface-Initiated Controlled Radical Polymerization Approach To Enhance Nanocomposite Integration of Cellulose Nanofibrils.
28482654	6	5	theme	strong	1017:1022	arg1	compatibility					1024:1036	a strong compatibility	1015:1036	a strong compatibility with the polypropylene matrix	1015:1066	Hydrophobic CNF showed a strong compatibility with the polypropylene matrix.
28482654	1	6	theme	nanofibrils	190:200	arg1	conversion					154:163	the conversion	150:163	the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix	150:327	A strategy is devised for the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix.
28482654	1	7	theme	functional	278:287	arg1	reinforcement					289:301	functional reinforcement	278:301	functional reinforcement of a polypropylene matrix	278:327	A strategy is devised for the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix.
28482654	5	8	theme	homogeneous	896:906	arg1	dispersion					908:917	a homogeneous dispersion	894:917	a homogeneous dispersion of the hydrophobic CNF in composite matrix with no signs of aggregation	894:989	Scanning electron microscopy reveals a homogeneous dispersion of the hydrophobic CNF in composite matrix with no signs of aggregation.
28482654	4	9	theme	melt	842:845	arg1	pressing					847:854	a twin-screw mini extruder and melt pressing	811:854	pressing	847:854	CNF-based nanocomposites were prepared using the combination of a twin-screw mini extruder and melt pressing.
28482654	2	10	theme	CNF-based	371:379	arg1	microinitiator					381:394	a CNF-based microinitiator	369:394	a CNF-based microinitiator	369:394	For that purpose, CNF was converted to a CNF-based microinitiator through an esterification reaction on the nanofibril surfaces, which efficiently initiated the controlled radical grafting polymerization of stearyl acrylate.
28482654	6	11	with	compatibility	1024:1036	arg1	matrix					1061:1066	the polypropylene matrix	1043:1066	the polypropylene matrix	1043:1066	Hydrophobic CNF showed a strong compatibility with the polypropylene matrix.
28482654	4	12	theme	CNF-based	747:755	arg1	nanocomposites					757:770	CNF-based nanocomposites	747:770	CNF-based nanocomposites	747:770	CNF-based nanocomposites were prepared using the combination of a twin-screw mini extruder and melt pressing.
28482654	6	13	theme	Hydrophobic	992:1002	arg1	CNF					1004:1006	Hydrophobic CNF	992:1006	Hydrophobic CNF	992:1006	Hydrophobic CNF showed a strong compatibility with the polypropylene matrix.
28482654	3	14	dep	transform	714:722	arg1	infrared					724:731	infrared	724:731	transform infrared spectroscopy	714:744	The grafting-from modification was performed with and without a sacrificial initiator and verified with solid-state 13C nuclear magnetic resonance and Fourier transform infrared spectroscopy.
28482654	5	15	theme	Scanning	857:864	arg1	microscopy					875:884	Scanning electron microscopy	857:884	Scanning electron microscopy	857:884	Scanning electron microscopy reveals a homogeneous dispersion of the hydrophobic CNF in composite matrix with no signs of aggregation.
28482654	0	16	theme	Controlled	18:27	arg1	Approach					52:59	Surface-Initiated Controlled Radical Polymerization Approach	0:59	Surface-Initiated Controlled Radical Polymerization Approach	0:59	Surface-Initiated Controlled Radical Polymerization Approach To Enhance Nanocomposite Integration of Cellulose Nanofibrils.
28482654	2	17	theme	esterification	407:420	arg1	reaction					422:429	an esterification reaction	404:429	an esterification reaction on the nanofibril surfaces, which efficiently initiated the controlled radical grafting polymerization of stearyl acrylate	404:552	For that purpose, CNF was converted to a CNF-based microinitiator through an esterification reaction on the nanofibril surfaces, which efficiently initiated the controlled radical grafting polymerization of stearyl acrylate.
28482654	0	18	theme	Surface-Initiated	0:16	arg1	Approach					52:59	Surface-Initiated Controlled Radical Polymerization Approach	0:59	Surface-Initiated Controlled Radical Polymerization Approach	0:59	Surface-Initiated Controlled Radical Polymerization Approach To Enhance Nanocomposite Integration of Cellulose Nanofibrils.
28482654	4	19	theme	extruder	829:836	arg1	combination					796:806	the combination	792:806	the combination of a twin-screw mini extruder and melt pressing	792:854	CNF-based nanocomposites were prepared using the combination of a twin-screw mini extruder and melt pressing.
28482654	5	20	theme	aggregation	979:989	arg1	signs					970:974	no signs	967:974	no signs of aggregation	967:989	Scanning electron microscopy reveals a homogeneous dispersion of the hydrophobic CNF in composite matrix with no signs of aggregation.
28482654	0	21	theme	Polymerization	37:50	arg1	Approach					52:59	Surface-Initiated Controlled Radical Polymerization Approach	0:59	Surface-Initiated Controlled Radical Polymerization Approach	0:59	Surface-Initiated Controlled Radical Polymerization Approach To Enhance Nanocomposite Integration of Cellulose Nanofibrils.
28482654	1	22	theme	polypropylene	308:320	arg1	matrix					322:327	a polypropylene matrix	306:327	a polypropylene matrix	306:327	A strategy is devised for the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix.
28482654	5	23	with	matrix	955:960	arg1	signs					970:974	no signs	967:974	no signs of aggregation	967:989	Scanning electron microscopy reveals a homogeneous dispersion of the hydrophobic CNF in composite matrix with no signs of aggregation.
28482654	4	24	theme	pressing	847:854	arg1	combination					796:806	the combination	792:806	the combination of a twin-screw mini extruder and melt pressing	792:854	CNF-based nanocomposites were prepared using the combination of a twin-screw mini extruder and melt pressing.
28482654	1	25	theme	hydrophobic	214:224	arg1	CNF					226:228	hydrophobic CNF	214:228	hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix	214:327	A strategy is devised for the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix.
28482654	0	26	theme	Radical	29:35	arg1	Approach					52:59	Surface-Initiated Controlled Radical Polymerization Approach	0:59	Surface-Initiated Controlled Radical Polymerization Approach	0:59	Surface-Initiated Controlled Radical Polymerization Approach To Enhance Nanocomposite Integration of Cellulose Nanofibrils.
28482654	1	27	theme	matrix	322:327	arg1	reinforcement					289:301	functional reinforcement	278:301	functional reinforcement of a polypropylene matrix	278:327	A strategy is devised for the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix.
28482654	3	28	theme	sacrificial	619:629	arg1	initiator					631:639	a sacrificial initiator	617:639	a sacrificial initiator	617:639	The grafting-from modification was performed with and without a sacrificial initiator and verified with solid-state 13C nuclear magnetic resonance and Fourier transform infrared spectroscopy.
28482654	5	29	theme	composite	945:953	arg1	matrix					955:960	composite matrix	945:960	composite matrix with no signs of aggregation	945:989	Scanning electron microscopy reveals a homogeneous dispersion of the hydrophobic CNF in composite matrix with no signs of aggregation.
28482654	3	30	theme	solid-state	659:669	arg1	resonance					692:700	solid-state 13C nuclear magnetic resonance	659:700	solid-state 13C nuclear magnetic resonance	659:700	The grafting-from modification was performed with and without a sacrificial initiator and verified with solid-state 13C nuclear magnetic resonance and Fourier transform infrared spectroscopy.
28482654	2	31	theme	nanofibril	438:447	arg1	surfaces					449:456	the nanofibril surfaces	434:456	the nanofibril surfaces	434:456	For that purpose, CNF was converted to a CNF-based microinitiator through an esterification reaction on the nanofibril surfaces, which efficiently initiated the controlled radical grafting polymerization of stearyl acrylate.
28482654	2	32	theme	grafting	510:517	arg1	polymerization					519:532	the controlled radical grafting polymerization	487:532	the controlled radical grafting polymerization of stearyl acrylate	487:552	For that purpose, CNF was converted to a CNF-based microinitiator through an esterification reaction on the nanofibril surfaces, which efficiently initiated the controlled radical grafting polymerization of stearyl acrylate.
28482654	3	33	theme	13C	671:673	arg1	resonance					692:700	solid-state 13C nuclear magnetic resonance	659:700	solid-state 13C nuclear magnetic resonance	659:700	The grafting-from modification was performed with and without a sacrificial initiator and verified with solid-state 13C nuclear magnetic resonance and Fourier transform infrared spectroscopy.
28482654	5	34	theme	hydrophobic	926:936	arg1	CNF					938:940	the hydrophobic CNF	922:940	the hydrophobic CNF	922:940	Scanning electron microscopy reveals a homogeneous dispersion of the hydrophobic CNF in composite matrix with no signs of aggregation.
28482654	0	35	theme	Nanocomposite	72:84	arg1	Integration					86:96	Nanocomposite Integration	72:96	Nanocomposite Integration of Cellulose Nanofibrils	72:121	Surface-Initiated Controlled Radical Polymerization Approach To Enhance Nanocomposite Integration of Cellulose Nanofibrils.
28482654	2	36	theme	acrylate	545:552	arg1	polymerization					519:532	the controlled radical grafting polymerization	487:532	the controlled radical grafting polymerization of stearyl acrylate	487:552	For that purpose, CNF was converted to a CNF-based microinitiator through an esterification reaction on the nanofibril surfaces, which efficiently initiated the controlled radical grafting polymerization of stearyl acrylate.
28482654	2	37	theme	controlled	491:500	arg1	polymerization					519:532	the controlled radical grafting polymerization	487:532	the controlled radical grafting polymerization of stearyl acrylate	487:552	For that purpose, CNF was converted to a CNF-based microinitiator through an esterification reaction on the nanofibril surfaces, which efficiently initiated the controlled radical grafting polymerization of stearyl acrylate.
28482654	6	38	theme	polypropylene	1047:1059	arg1	matrix					1061:1066	the polypropylene matrix	1043:1066	the polypropylene matrix	1043:1066	Hydrophobic CNF showed a strong compatibility with the polypropylene matrix.
28482654	3	39	theme	grafting-from	559:571	arg1	modification					573:584	The grafting-from modification	555:584	The grafting-from modification	555:584	The grafting-from modification was performed with and without a sacrificial initiator and verified with solid-state 13C nuclear magnetic resonance and Fourier transform infrared spectroscopy.
28482654	5	40	from	dispersion	908:917	arg1	matrix					955:960	composite matrix	945:960	composite matrix with no signs of aggregation	945:989	Scanning electron microscopy reveals a homogeneous dispersion of the hydrophobic CNF in composite matrix with no signs of aggregation.
28482654	2	41	from	reaction	422:429	arg1	surfaces					449:456	the nanofibril surfaces	434:456	the nanofibril surfaces	434:456	For that purpose, CNF was converted to a CNF-based microinitiator through an esterification reaction on the nanofibril surfaces, which efficiently initiated the controlled radical grafting polymerization of stearyl acrylate.
28482654	4	42	theme	mini	824:827	arg1	extruder					829:836	a twin-screw mini extruder and melt pressing	811:854	extruder	829:836	CNF-based nanocomposites were prepared using the combination of a twin-screw mini extruder and melt pressing.
28482654	3	43	dep	Fourier	706:712	arg1	transform					714:722	transform	714:722	transform infrared spectroscopy	714:744	The grafting-from modification was performed with and without a sacrificial initiator and verified with solid-state 13C nuclear magnetic resonance and Fourier transform infrared spectroscopy.
28482654	5	44	theme	CNF	938:940	arg1	dispersion					908:917	a homogeneous dispersion	894:917	a homogeneous dispersion of the hydrophobic CNF in composite matrix with no signs of aggregation	894:989	Scanning electron microscopy reveals a homogeneous dispersion of the hydrophobic CNF in composite matrix with no signs of aggregation.
28482654	4	45	theme	twin-screw	813:822	arg1	extruder					829:836	a twin-screw mini extruder and melt pressing	811:854	extruder	829:836	CNF-based nanocomposites were prepared using the combination of a twin-screw mini extruder and melt pressing.
28482654	1	46	theme	stable	242:247	arg1	dispersion					263:272	a stable nanocomposite dispersion	240:272	a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix	240:327	A strategy is devised for the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix.
28482654	3	47	theme	nuclear	675:681	arg1	resonance					692:700	solid-state 13C nuclear magnetic resonance	659:700	solid-state 13C nuclear magnetic resonance	659:700	The grafting-from modification was performed with and without a sacrificial initiator and verified with solid-state 13C nuclear magnetic resonance and Fourier transform infrared spectroscopy.
28482654	1	48	theme	nanocomposite	249:261	arg1	dispersion					263:272	a stable nanocomposite dispersion	240:272	a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix	240:327	A strategy is devised for the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix.
28482654	2	49	theme	radical	502:508	arg1	polymerization					519:532	the controlled radical grafting polymerization	487:532	the controlled radical grafting polymerization of stearyl acrylate	487:552	For that purpose, CNF was converted to a CNF-based microinitiator through an esterification reaction on the nanofibril surfaces, which efficiently initiated the controlled radical grafting polymerization of stearyl acrylate.
28482654	3	50	theme	magnetic	683:690	arg1	resonance					692:700	solid-state 13C nuclear magnetic resonance	659:700	solid-state 13C nuclear magnetic resonance	659:700	The grafting-from modification was performed with and without a sacrificial initiator and verified with solid-state 13C nuclear magnetic resonance and Fourier transform infrared spectroscopy.
28482654	1	51	theme	hydrophilic	168:178	arg1	CNFs					203:206	CNFs	203:206	CNFs	203:206	A strategy is devised for the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix.
28482654	1	51	theme	hydrophilic	168:178	arg1	nanofibrils					190:200	hydrophilic cellulose nanofibrils	168:200	hydrophilic cellulose nanofibrils (CNFs)	168:207	A strategy is devised for the conversion of hydrophilic cellulose nanofibrils (CNFs) into hydrophobic CNF that form a stable nanocomposite dispersion for functional reinforcement of a polypropylene matrix.
25443671	0	0	theme	isolated	67:74	arg1	microalgae					76:85	newly isolated microalgae	61:85	newly isolated microalgae	61:85	Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition.
25443671	1	1	with	isolate	210:216	arg1	content					234:240	high lipid content	223:240	high lipid content	223:240	In order to produce microalgal lipids that can be transformed to biodiesel fuel, one isolate with high lipid content was identified as Chlorella sp.
25443671	1	2	theme	microalgal	145:154	arg1	lipids					156:161	microalgal lipids	145:161	microalgal lipids that can be transformed to biodiesel fuel	145:203	In order to produce microalgal lipids that can be transformed to biodiesel fuel, one isolate with high lipid content was identified as Chlorella sp.
25443671	4	3	theme	carbon	542:547	arg1	sources					549:555	organic carbon sources	534:555	organic carbon sources	534:555	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	3	4	theme	lipid	295:299	arg1	productivity					301:312	lipid productivity	295:312	lipid productivity	295:312	The growth and lipid productivity of an isolated microalga Chlorella sp.
25443671	8	5	theme	Fatty	801:805	arg1	analysis					812:819	Fatty acid analysis	801:819	Fatty acid analysis of Chlorella sp	801:835	Fatty acid analysis of Chlorella sp.
25443671	4	6	theme	organic	534:540	arg1	sources					549:555	organic carbon sources	534:555	organic carbon sources	534:555	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	3	7	theme	microalga	329:337	arg1	sp					349:350	an isolated microalga Chlorella sp	317:350	an isolated microalga Chlorella sp	317:350	The growth and lipid productivity of an isolated microalga Chlorella sp.
25443671	0	8	from	Effect	0:5	arg1	growth					28:33	growth	28:33	growth	28:33	Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition.
25443671	0	8	from	Effect	0:5	arg1	accumulation					45:56	lipid accumulation	39:56	lipid accumulation	39:56	Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition.
25443671	11	9	theme	biodiesel	1045:1053	arg1	production					1055:1064	biodiesel production	1045:1064	biodiesel production	1045:1064	Y8-1 are appropriate for biodiesel production.
25443671	6	10	theme	higher	654:659	arg1	productivity					667:678	higher lipid productivity	654:678	higher lipid productivity (0.01 g/L/d)	654:691	Y8-1 showed higher lipid content (35.5±4.2%) and higher lipid productivity (0.01 g/L/d) than Chlorella sp.
25443671	6	10	theme	higher	654:659	arg1	g/L/d					686:690	0.01 g/L/d	681:690	0.01 g/L/d	681:690	Y8-1 showed higher lipid content (35.5±4.2%) and higher lipid productivity (0.01 g/L/d) than Chlorella sp.
25443671	3	11	theme	Chlorella	339:347	arg1	sp					349:350	an isolated microalga Chlorella sp	317:350	an isolated microalga Chlorella sp	317:350	The growth and lipid productivity of an isolated microalga Chlorella sp.
25443671	10	12	theme	acid	981:984	arg1	compositions					986:997	The main fatty acid compositions	966:997	The main fatty acid compositions of the Chlorella sp	966:1017	The main fatty acid compositions of the Chlorella sp.
25443671	9	13	theme	oleic	895:899	arg1	C18:1					907:911	C18:1	907:911	C18:1	907:911	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	9	13	theme	oleic	895:899	arg1	acid					901:904	oleic acid	895:904	oleic acid (C18:1)	895:912	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	4	14	theme	cultivation	392:402	arg1	growth					478:483	autotrophic growth (CO2, with light), heterotrophic growth	426:483	autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light)	426:508	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	4	14	theme	cultivation	392:402	arg1	conditions					404:413	different cultivation conditions	382:413	different cultivation conditions	382:413	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	4	14	theme	cultivation	392:402	arg1	growth					526:531	mixotrophic growth	514:531	mixotrophic growth (organic carbon sources and CO2, with light)	514:576	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	7	15	theme	heterotrophic	750:762	arg1	conditions					764:773	autotrophic and heterotrophic conditions	734:773	autotrophic and heterotrophic conditions	734:773	Y8-1 cultivated under autotrophic and heterotrophic conditions on modified Walne medium.
25443671	4	16	dep	growth	438:443	arg1	autotrophic					426:436	autotrophic	426:436	autotrophic	426:436	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	4	16	dep	growth	438:443	arg1	CO2					446:448	CO2	446:448	CO2	446:448	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	4	16	dep	growth	438:443	arg1	heterotrophic					464:476	heterotrophic	464:476	heterotrophic	464:476	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	0	17	theme	mixotrophic	102:112	arg1	condition					114:122	mixotrophic condition	102:122	mixotrophic condition	102:122	Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition.
25443671	4	18	dep	growth	526:531	arg1	sources					549:555	organic carbon sources	534:555	organic carbon sources	534:555	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	4	18	dep	growth	526:531	arg1	CO2					561:563	CO2	561:563	CO2	561:563	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	4	19	theme	different	382:390	arg1	growth					478:483	autotrophic growth (CO2, with light), heterotrophic growth	426:483	autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light)	426:508	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	4	19	theme	different	382:390	arg1	conditions					404:413	different cultivation conditions	382:413	different cultivation conditions	382:413	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	4	19	theme	different	382:390	arg1	growth					526:531	mixotrophic growth	514:531	mixotrophic growth (organic carbon sources and CO2, with light)	514:576	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	8	20	theme	acid	807:810	arg1	analysis					812:819	Fatty acid analysis	801:819	Fatty acid analysis of Chlorella sp	801:835	Fatty acid analysis of Chlorella sp.
25443671	3	21	theme	isolated	320:327	arg1	sp					349:350	an isolated microalga Chlorella sp	317:350	an isolated microalga Chlorella sp	317:350	The growth and lipid productivity of an isolated microalga Chlorella sp.
25443671	10	22	theme	main	970:973	arg1	compositions					986:997	The main fatty acid compositions	966:997	The main fatty acid compositions of the Chlorella sp	966:1017	The main fatty acid compositions of the Chlorella sp.
25443671	1	23	theme	lipid	228:232	arg1	content					234:240	high lipid content	223:240	high lipid content	223:240	In order to produce microalgal lipids that can be transformed to biodiesel fuel, one isolate with high lipid content was identified as Chlorella sp.
25443671	7	24	theme	Walne	787:791	arg1	medium					793:798	modified Walne medium	778:798	modified Walne medium	778:798	Y8-1 cultivated under autotrophic and heterotrophic conditions on modified Walne medium.
25443671	4	25	with	CO2	446:448	arg1	light					456:460	light	456:460	light	456:460	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	5	26	theme	Mixotrophic	579:589	arg1	sp					601:602	Mixotrophic Chlorella sp	579:602	Mixotrophic Chlorella sp.	579:603	Mixotrophic Chlorella sp.
25443671	7	27	theme	autotrophic	734:744	arg1	conditions					764:773	autotrophic and heterotrophic conditions	734:773	autotrophic and heterotrophic conditions	734:773	Y8-1 cultivated under autotrophic and heterotrophic conditions on modified Walne medium.
25443671	10	28	theme	fatty	975:979	arg1	compositions					986:997	The main fatty acid compositions	966:997	The main fatty acid compositions of the Chlorella sp	966:1017	The main fatty acid compositions of the Chlorella sp.
25443671	4	29	theme	mixotrophic	514:524	arg1	growth					526:531	mixotrophic growth	514:531	mixotrophic growth (organic carbon sources and CO2, with light)	514:576	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	5	30	theme	Chlorella	591:599	arg1	sp					601:602	Mixotrophic Chlorella sp	579:602	Mixotrophic Chlorella sp.	579:603	Mixotrophic Chlorella sp.
25443671	10	31	theme	Chlorella	1006:1014	arg1	sp					1016:1017	the Chlorella sp	1002:1017	the Chlorella sp	1002:1017	The main fatty acid compositions of the Chlorella sp.
25443671	3	32	theme	sp	349:350	arg1	productivity					301:312	lipid productivity	295:312	lipid productivity	295:312	The growth and lipid productivity of an isolated microalga Chlorella sp.
25443671	3	32	theme	sp	349:350	arg1	growth					284:289	growth	284:289	growth	284:289	The growth and lipid productivity of an isolated microalga Chlorella sp.
25443671	1	33	theme	Chlorella	260:268	arg1	sp					270:271	Chlorella sp	260:271	Chlorella sp	260:271	In order to produce microalgal lipids that can be transformed to biodiesel fuel, one isolate with high lipid content was identified as Chlorella sp.
25443671	1	33	theme	Chlorella	260:268	arg1	isolate					210:216	one isolate	206:216	one isolate with high lipid content	206:240	In order to produce microalgal lipids that can be transformed to biodiesel fuel, one isolate with high lipid content was identified as Chlorella sp.
25443671	6	34	theme	Chlorella	698:706	arg1	sp					708:709	Chlorella sp	698:709	Chlorella sp	698:709	Y8-1 showed higher lipid content (35.5±4.2%) and higher lipid productivity (0.01 g/L/d) than Chlorella sp.
25443671	7	35	theme	modified	778:785	arg1	medium					793:798	modified Walne medium	778:798	modified Walne medium	778:798	Y8-1 cultivated under autotrophic and heterotrophic conditions on modified Walne medium.
25443671	9	36	theme	acid	901:904	arg1	presence					860:867	the major presence	850:867	the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3)	850:963	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	0	37	theme	carbon	10:15	arg1	sources					17:23	carbon sources	10:23	carbon sources	10:23	Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition.
25443671	9	38	theme	acid	881:884	arg1	presence					860:867	the major presence	850:867	the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3)	850:963	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	6	39	theme	lipid	624:628	arg1	content					630:636	higher lipid content	617:636	higher lipid content (35.5±4.2%)	617:648	Y8-1 showed higher lipid content (35.5±4.2%) and higher lipid productivity (0.01 g/L/d) than Chlorella sp.
25443671	6	39	theme	lipid	624:628	arg1	%					647:647	35.5±4.2%	639:647	35.5±4.2%	639:647	Y8-1 showed higher lipid content (35.5±4.2%) and higher lipid productivity (0.01 g/L/d) than Chlorella sp.
25443671	4	40	theme	growth	438:443	arg1	growth					478:483	autotrophic growth (CO2, with light), heterotrophic growth	426:483	autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light)	426:508	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	9	41	theme	linolenic	941:949	arg1	C18:3					958:962	C18:3	958:962	C18:3	958:962	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	9	41	theme	linolenic	941:949	arg1	acids					951:955	linolenic acids	941:955	linolenic acids (C18:3)	941:963	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	6	42	theme	higher	617:622	arg1	content					630:636	higher lipid content	617:636	higher lipid content (35.5±4.2%)	617:648	Y8-1 showed higher lipid content (35.5±4.2%) and higher lipid productivity (0.01 g/L/d) than Chlorella sp.
25443671	6	42	theme	higher	617:622	arg1	%					647:647	35.5±4.2%	639:647	35.5±4.2%	639:647	Y8-1 showed higher lipid content (35.5±4.2%) and higher lipid productivity (0.01 g/L/d) than Chlorella sp.
25443671	0	43	theme	sources	17:23	arg1	Effect					0:5	Effect	0:5	Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae	0:85	Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition.
25443671	0	44	theme	lipid	39:43	arg1	accumulation					45:56	lipid accumulation	39:56	lipid accumulation	39:56	Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition.
25443671	9	45	theme	palmitic	872:879	arg1	acid					881:884	palmitic acid	872:884	palmitic acid (C16:0)	872:892	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	9	45	theme	palmitic	872:879	arg1	C16:0					887:891	C16:0	887:891	C16:0	887:891	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	4	46	dep	growth	478:483	arg1	sucrose					486:492	sucrose	486:492	sucrose	486:492	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	4	47	with	CO2	561:563	arg1	light					571:575	light	571:575	light	571:575	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	9	48	theme	linoleic	915:922	arg1	C18:2					930:934	C18:2	930:934	C18:2	930:934	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	9	48	theme	linoleic	915:922	arg1	acid					924:927	linoleic acid	915:927	linoleic acid (C18:2)	915:935	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	10	49	theme	sp	1016:1017	arg1	compositions					986:997	The main fatty acid compositions	966:997	The main fatty acid compositions of the Chlorella sp	966:1017	The main fatty acid compositions of the Chlorella sp.
25443671	9	50	theme	acid	924:927	arg1	presence					860:867	the major presence	850:867	the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3)	850:963	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	4	51	with	sources	549:555	arg1	light					571:575	light	571:575	light	571:575	Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light).
25443671	8	52	theme	sp	834:835	arg1	analysis					812:819	Fatty acid analysis	801:819	Fatty acid analysis of Chlorella sp	801:835	Fatty acid analysis of Chlorella sp.
25443671	8	53	theme	Chlorella	824:832	arg1	sp					834:835	Chlorella sp	824:835	Chlorella sp	824:835	Fatty acid analysis of Chlorella sp.
25443671	9	54	theme	acids	951:955	arg1	presence					860:867	the major presence	850:867	the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3)	850:963	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	9	55	theme	major	854:858	arg1	presence					860:867	the major presence	850:867	the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3)	850:963	Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3).
25443671	1	56	theme	high	223:226	arg1	content					234:240	high lipid content	223:240	high lipid content	223:240	In order to produce microalgal lipids that can be transformed to biodiesel fuel, one isolate with high lipid content was identified as Chlorella sp.
25443671	6	57	theme	lipid	661:665	arg1	productivity					667:678	higher lipid productivity	654:678	higher lipid productivity (0.01 g/L/d)	654:691	Y8-1 showed higher lipid content (35.5±4.2%) and higher lipid productivity (0.01 g/L/d) than Chlorella sp.
25443671	6	57	theme	lipid	661:665	arg1	g/L/d					686:690	0.01 g/L/d	681:690	0.01 g/L/d	681:690	Y8-1 showed higher lipid content (35.5±4.2%) and higher lipid productivity (0.01 g/L/d) than Chlorella sp.
25443671	0	58	theme	microalgae	76:85	arg1	growth					28:33	growth	28:33	growth	28:33	Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition.
25443671	0	58	theme	microalgae	76:85	arg1	accumulation					45:56	lipid accumulation	39:56	lipid accumulation	39:56	Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition.
27810877	7	0	theme	relative	1349:1356	arg1	abundance					1358:1366	the relative abundance	1345:1366	the relative abundance of specific microbial genera and the immune response	1345:1419	Thus, MOS and XOS differently changed the relative abundance of specific microbial genera and the immune response during infection, and these changes were correlated with their abilities to reduce S. Enteritidis colonisation.
27810877	2	1	from	expression	423:432	arg1	chickens					450:457	chickens	450:457	chickens infected with S. Enteritidis	450:486	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	0	2	theme	cytokines	99:107	arg1	microbiota					49:58	caecal microbiota	42:58	caecal microbiota	42:58	Mannan- and xylooligosaccharides modulate caecal microbiota and expression of inflammatory-related cytokines and reduce caecal Salmonella Enteritidis colonisation in young chickens.
27810877	0	2	theme	cytokines	99:107	arg1	expression					64:73	expression	64:73	expression	64:73	Mannan- and xylooligosaccharides modulate caecal microbiota and expression of inflammatory-related cytokines and reduce caecal Salmonella Enteritidis colonisation in young chickens.
27810877	7	3	dep	S.	1504:1505	arg1	Enteritidis					1507:1517	Enteritidis	1507:1517	Enteritidis	1507:1517	Thus, MOS and XOS differently changed the relative abundance of specific microbial genera and the immune response during infection, and these changes were correlated with their abilities to reduce S. Enteritidis colonisation.
27810877	3	4	dep	control	562:568	arg1	1					548:548	1	548:548	1	548:548	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	5	5	theme	interferon-γ	1113:1124	arg1	increase					1034:1041	the increase	1030:1041	the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils	1030:1142	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	5	6	theme	caecal	1129:1134	arg1	tonsils					1136:1142	caecal tonsils	1129:1142	caecal tonsils	1129:1142	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	0	7	theme	caecal	120:125	arg1	colonisation					150:161	caecal Salmonella Enteritidis colonisation	120:161	caecal Salmonella Enteritidis colonisation in young chickens	120:179	Mannan- and xylooligosaccharides modulate caecal microbiota and expression of inflammatory-related cytokines and reduce caecal Salmonella Enteritidis colonisation in young chickens.
27810877	3	8	theme	infected	617:624	arg1	MOS					628:630	MOS	628:630	MOS	628:630	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	3	8	theme	infected	617:624	arg1	one					527:529	one	527:529	one	527:529	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	3	8	theme	infected	617:624	arg1	groups					539:544	five groups	534:544	five groups: (1) uninfected control, (2) infected control, (3) infected + XOS	534:610	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	6	9	theme	genus	1294:1298	arg1	level					1300:1304	the genus level	1290:1304	the genus level	1290:1304	The canonical correspondence analysis for cytokine genes showed a correlation with the composition of the microbial community at the genus level.
27810877	6	10	with	correlation	1227:1237	arg1	composition					1248:1258	the composition	1244:1258	the composition of the microbial community at the genus level	1244:1304	The canonical correspondence analysis for cytokine genes showed a correlation with the composition of the microbial community at the genus level.
27810877	7	11	theme	response	1412:1419	arg1	abundance					1358:1366	the relative abundance	1345:1366	the relative abundance of specific microbial genera and the immune response	1345:1419	Thus, MOS and XOS differently changed the relative abundance of specific microbial genera and the immune response during infection, and these changes were correlated with their abilities to reduce S. Enteritidis colonisation.
27810877	0	12	dep	Salmonella	127:136	arg1	Enteritidis					138:148	Enteritidis	138:148	Enteritidis	138:148	Mannan- and xylooligosaccharides modulate caecal microbiota and expression of inflammatory-related cytokines and reduce caecal Salmonella Enteritidis colonisation in young chickens.
27810877	5	13	theme	lipopolysaccharide-induced	1046:1071	arg1	alpha					1096:1100	lipopolysaccharide-induced tumour necrosis factor alpha	1046:1100	lipopolysaccharide-induced tumour necrosis factor alpha factor	1046:1107	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	2	14	theme	caecal	401:406	arg1	microbiota					408:417	caecal microbiota	401:417	caecal microbiota	401:417	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	0	15	theme	Salmonella	127:136	arg1	colonisation					150:161	caecal Salmonella Enteritidis colonisation	120:161	caecal Salmonella Enteritidis colonisation in young chickens	120:179	Mannan- and xylooligosaccharides modulate caecal microbiota and expression of inflammatory-related cytokines and reduce caecal Salmonella Enteritidis colonisation in young chickens.
27810877	5	16	theme	XOS	949:951	arg1	XOS					1016:1018	XOS	1016:1018	XOS	1016:1018	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	5	16	theme	XOS	949:951	arg1	Roseburia					993:1001	Roseburia	993:1001	Roseburia	993:1001	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	5	16	theme	XOS	949:951	arg1	Lactobacillus					975:987	Lactobacillus	975:987	Lactobacillus	975:987	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	5	16	theme	XOS	949:951	arg1	Clostridium					962:972	XOS enriched Clostridium	949:972	XOS enriched Clostridium	949:972	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	3	17	theme	groups	539:544	arg1	virginiamycin					651:663	virginiamycin	651:663	virginiamycin	651:663	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	3	17	theme	groups	539:544	arg1	infected					640:647	infected	640:647	infected	640:647	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	3	17	theme	groups	539:544	arg1	MOS					628:630	MOS	628:630	MOS	628:630	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	3	17	theme	groups	539:544	arg1	one					527:529	one	527:529	one	527:529	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	3	17	theme	groups	539:544	arg1	groups					539:544	five groups	534:544	five groups: (1) uninfected control, (2) infected control, (3) infected + XOS	534:610	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	5	18	theme	enriched	953:960	arg1	XOS					1016:1018	XOS	1016:1018	XOS	1016:1018	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	5	18	theme	enriched	953:960	arg1	Roseburia					993:1001	Roseburia	993:1001	Roseburia	993:1001	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	5	18	theme	enriched	953:960	arg1	Lactobacillus					975:987	Lactobacillus	975:987	Lactobacillus	975:987	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	5	18	theme	enriched	953:960	arg1	Clostridium					962:972	XOS enriched Clostridium	949:972	XOS enriched Clostridium	949:972	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	7	19	theme	S.	1504:1505	arg1	colonisation					1519:1530	S. Enteritidis colonisation	1504:1530	S. Enteritidis colonisation	1504:1530	Thus, MOS and XOS differently changed the relative abundance of specific microbial genera and the immune response during infection, and these changes were correlated with their abilities to reduce S. Enteritidis colonisation.
27810877	3	20	theme	uninfected	551:560	arg1	control					562:568	(1) uninfected control	547:568	(1) uninfected control	547:568	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	6	21	theme	canonical	1165:1173	arg1	analysis					1190:1197	The canonical correspondence analysis	1161:1197	The canonical correspondence analysis for cytokine genes	1161:1216	The canonical correspondence analysis for cytokine genes showed a correlation with the composition of the microbial community at the genus level.
27810877	5	22	dep	Lactobacillus	975:987	arg1	MOS					1003:1005	MOS	1003:1005	MOS	1003:1005	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	7	23	theme	specific	1371:1378	arg1	genera					1390:1395	specific microbial genera	1371:1395	specific microbial genera	1371:1395	Thus, MOS and XOS differently changed the relative abundance of specific microbial genera and the immune response during infection, and these changes were correlated with their abilities to reduce S. Enteritidis colonisation.
27810877	6	24	theme	cytokine	1203:1210	arg1	genes					1212:1216	cytokine genes	1203:1216	cytokine genes	1203:1216	The canonical correspondence analysis for cytokine genes showed a correlation with the composition of the microbial community at the genus level.
27810877	2	25	theme	prebiotics	321:330	arg1	impact					297:302	the impact	293:302	the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis	293:486	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	2	26	theme	cytokines	437:445	arg1	expression					423:432	expression	423:432	expression	423:432	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	2	26	theme	cytokines	437:445	arg1	microbiota					408:417	caecal microbiota	401:417	caecal microbiota	401:417	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	4	27	theme	less	779:782	arg1	extent					784:789	a less extent (1.0 log)	777:799	a less extent (1.0 log)	777:799	The number of S. Enteritidis recovered from the caecum was significantly lower, by 1.6 log, in the MOS, and to a less extent (1.0 log) in the XOS-fed birds compared to the infected control.
27810877	4	27	theme	less	779:782	arg1	log					796:798	1.0 log	792:798	1.0 log	792:798	The number of S. Enteritidis recovered from the caecum was significantly lower, by 1.6 log, in the MOS, and to a less extent (1.0 log) in the XOS-fed birds compared to the infected control.
27810877	6	28	from	level	1300:1304	arg1	composition					1248:1258	the composition	1244:1258	the composition of the microbial community at the genus level	1244:1304	The canonical correspondence analysis for cytokine genes showed a correlation with the composition of the microbial community at the genus level.
27810877	2	29	theme	potential	311:319	arg1	prebiotics					321:330	two potential prebiotics	307:330	two potential prebiotics	307:330	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	2	29	theme	potential	311:319	arg1	xylooligosaccharides					366:385	xylooligosaccharides	366:385	xylooligosaccharides (XOS)	366:391	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	2	29	theme	potential	311:319	arg1	mannanoligosaccharides					333:354	mannanoligosaccharides	333:354	mannanoligosaccharides (MOS)	333:360	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	3	30	dep	control	584:590	arg1	2					572:572	2	572:572	2	572:572	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	4	31	theme	infected	838:845	arg1	control					847:853	the infected control	834:853	the infected control	834:853	The number of S. Enteritidis recovered from the caecum was significantly lower, by 1.6 log, in the MOS, and to a less extent (1.0 log) in the XOS-fed birds compared to the infected control.
27810877	7	32	theme	immune	1405:1410	arg1	response					1412:1419	the immune response	1401:1419	the immune response	1401:1419	Thus, MOS and XOS differently changed the relative abundance of specific microbial genera and the immune response during infection, and these changes were correlated with their abilities to reduce S. Enteritidis colonisation.
27810877	0	33	theme	caecal	42:47	arg1	microbiota					49:58	caecal microbiota	42:58	caecal microbiota	42:58	Mannan- and xylooligosaccharides modulate caecal microbiota and expression of inflammatory-related cytokines and reduce caecal Salmonella Enteritidis colonisation in young chickens.
27810877	0	34	theme	young	166:170	arg1	chickens					172:179	young chickens	166:179	young chickens	166:179	Mannan- and xylooligosaccharides modulate caecal microbiota and expression of inflammatory-related cytokines and reduce caecal Salmonella Enteritidis colonisation in young chickens.
27810877	7	35	theme	genera	1390:1395	arg1	abundance					1358:1366	the relative abundance	1345:1366	the relative abundance of specific microbial genera and the immune response	1345:1419	Thus, MOS and XOS differently changed the relative abundance of specific microbial genera and the immune response during infection, and these changes were correlated with their abilities to reduce S. Enteritidis colonisation.
27810877	2	36	dep	microbiota	408:417	arg1	the					397:399	the	397:399	the	397:399	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	6	37	theme	community	1277:1285	arg1	composition					1248:1258	the composition	1244:1258	the composition of the microbial community at the genus level	1244:1304	The canonical correspondence analysis for cytokine genes showed a correlation with the composition of the microbial community at the genus level.
27810877	6	38	theme	correspondence	1175:1188	arg1	analysis					1190:1197	The canonical correspondence analysis	1161:1197	The canonical correspondence analysis for cytokine genes	1161:1216	The canonical correspondence analysis for cytokine genes showed a correlation with the composition of the microbial community at the genus level.
27810877	5	39	from	increase	1034:1041	arg1	tonsils					1136:1142	caecal tonsils	1129:1142	caecal tonsils	1129:1142	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	5	40	dep	MOS	936:938	arg1	response					924:931	response	924:931	response	924:931	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	5	41	theme	Enterococcus	886:897	arg1	genera					899:904	Enterococcus genera	886:904	Enterococcus genera	886:904	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	3	42	dep	groups	539:544	arg1	infected					597:604	infected	597:604	infected	597:604	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	3	42	dep	groups	539:544	arg1	control					584:590	(2) infected control	571:590	(2) infected control	571:590	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	3	42	dep	groups	539:544	arg1	XOS					608:610	XOS	608:610	XOS	608:610	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	3	42	dep	groups	539:544	arg1	control					562:568	(1) uninfected control	547:568	(1) uninfected control	547:568	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	2	43	from	impact	297:302	arg1	expression					423:432	expression	423:432	expression	423:432	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	2	43	from	impact	297:302	arg1	microbiota					408:417	caecal microbiota	401:417	caecal microbiota	401:417	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	5	44	dep	increased	911:919	arg1	whereas					941:947	whereas	941:947	whereas	941:947	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	7	45	theme	microbial	1380:1388	arg1	genera					1390:1395	specific microbial genera	1371:1395	specific microbial genera	1371:1395	Thus, MOS and XOS differently changed the relative abundance of specific microbial genera and the immune response during infection, and these changes were correlated with their abilities to reduce S. Enteritidis colonisation.
27810877	5	46	theme	tumour	1073:1078	arg1	alpha					1096:1100	lipopolysaccharide-induced tumour necrosis factor alpha	1046:1100	lipopolysaccharide-induced tumour necrosis factor alpha factor	1046:1107	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	4	47	theme	Enteritidis	683:693	arg1	number					670:675	The number	666:675	The number of S. Enteritidis recovered from the caecum	666:719	The number of S. Enteritidis recovered from the caecum was significantly lower, by 1.6 log, in the MOS, and to a less extent (1.0 log) in the XOS-fed birds compared to the infected control.
27810877	4	47	theme	Enteritidis	683:693	arg1	lower					739:743	lower	739:743	lower	739:743	The number of S. Enteritidis recovered from the caecum was significantly lower, by 1.6 log, in the MOS, and to a less extent (1.0 log) in the XOS-fed birds compared to the infected control.
27810877	3	48	theme	hatched	495:501	arg1	chicks					503:508	Newly hatched chicks	489:508	Newly hatched chicks	489:508	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	5	49	theme	necrosis	1080:1087	arg1	alpha					1096:1100	lipopolysaccharide-induced tumour necrosis factor alpha	1046:1100	lipopolysaccharide-induced tumour necrosis factor alpha factor	1046:1107	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	0	50	theme	inflammatory-related	78:97	arg1	cytokines					99:107	inflammatory-related cytokines	78:107	inflammatory-related cytokines	78:107	Mannan- and xylooligosaccharides modulate caecal microbiota and expression of inflammatory-related cytokines and reduce caecal Salmonella Enteritidis colonisation in young chickens.
27810877	3	51	theme	infected	575:582	arg1	control					584:590	(2) infected control	571:590	(2) infected control	571:590	Newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + XOS, (4) infected + MOS and (5) infected + virginiamycin.
27810877	2	52	from	microbiota	408:417	arg1	chickens					450:457	chickens	450:457	chickens infected with S. Enteritidis	450:486	This study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (MOS) and xylooligosaccharides (XOS), on the caecal microbiota and expression of cytokines in chickens infected with S. Enteritidis.
27810877	6	53	theme	microbial	1267:1275	arg1	community					1277:1285	the microbial community	1263:1285	the microbial community	1263:1285	The canonical correspondence analysis for cytokine genes showed a correlation with the composition of the microbial community at the genus level.
27810877	5	54	theme	factor	1089:1094	arg1	alpha					1096:1100	lipopolysaccharide-induced tumour necrosis factor alpha	1046:1100	lipopolysaccharide-induced tumour necrosis factor alpha factor	1046:1107	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	4	55	theme	XOS-fed	808:814	arg1	birds					816:820	the XOS-fed birds	804:820	the XOS-fed birds compared to the infected control	804:853	The number of S. Enteritidis recovered from the caecum was significantly lower, by 1.6 log, in the MOS, and to a less extent (1.0 log) in the XOS-fed birds compared to the infected control.
27810877	4	56	from	MOS	765:767	arg1	birds					816:820	the XOS-fed birds	804:820	the XOS-fed birds compared to the infected control	804:853	The number of S. Enteritidis recovered from the caecum was significantly lower, by 1.6 log, in the MOS, and to a less extent (1.0 log) in the XOS-fed birds compared to the infected control.
27810877	5	57	theme	alpha	1096:1100	arg1	factor					1102:1107	lipopolysaccharide-induced tumour necrosis factor alpha factor	1046:1107	lipopolysaccharide-induced tumour necrosis factor alpha factor	1046:1107	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	5	58	theme	factor	1102:1107	arg1	increase					1034:1041	the increase	1030:1041	the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils	1030:1142	Coprococcus, Ruminococcus and Enterococcus genera were increased in response to MOS, whereas XOS enriched Clostridium, Lactobacillus and Roseburia MOS, but not XOS, lessened the increase of lipopolysaccharide-induced tumour necrosis factor alpha factor and interferon-γ in caecal tonsils after challenge.
27810877	0	59	from	colonisation	150:161	arg1	chickens					172:179	young chickens	166:179	young chickens	166:179	Mannan- and xylooligosaccharides modulate caecal microbiota and expression of inflammatory-related cytokines and reduce caecal Salmonella Enteritidis colonisation in young chickens.
28066722	6	0	from	variation	882:890	arg1	fitness					907:913	competitive fitness	895:913	competitive fitness	895:913	Whereas a large variation in competitive fitness was found among mutants, no correlation was observed between GI fitness and total levels of individual cell wall components.
28066722	7	1	theme	distantly-related	1082:1098	arg1	species					1108:1114	distantly-related Candida species	1082:1114	distantly-related Candida species	1082:1114	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	2	2	theme	human	273:277	arg1	tract					301:305	The human gastrointestinal (GI) tract	269:305	The human gastrointestinal (GI) tract	269:305	The human gastrointestinal (GI) tract represents the largest natural reservoir of Candida species and is a major source of systemic fungal infections.
28066722	1	3	theme	%	266:266	arg1	rate					253:256	an associated mortality rate	229:256	an associated mortality rate of 46-75%	229:266	Candida albicans is responsible for ~400,000 systemic fungal infections annually, with an associated mortality rate of 46-75%.
28066722	9	4	theme	GI	1618:1619	arg1	fitness					1621:1627	GI fitness	1618:1627	GI fitness	1618:1627	The most significant association with GI fitness was found to be the strength of signaling through the Dectin-1 receptor.
28066722	4	5	theme	species	641:647	arg1	fitness					622:628	the competitive fitness	606:628	the competitive fitness of Candida species in the mammalian GI tract	606:673	We hypothesized that the fungal cell wall would play an important role in determining the competitive fitness of Candida species in the mammalian GI tract.
28066722	11	6	dep	architecture	1971:1982	arg1	so					1990:1991	so	1990:1991	so	1990:1991	These data suggest that fungal cell wall architecture, more so than its crude composition, critically determines the ability of fungi to colonize the mammalian GI tract.
28066722	5	7	dep	C.	750:751	arg1	albicans					753:760	albicans	753:760	albicans	753:760	To test this hypothesis, we generated a systematic collection of isogenic C. albicans cell wall mutants and measured their fitness in the mouse GI tract via quantitative competition assays.
28066722	1	8	theme	systemic	187:194	arg1	infections					203:212	~400,000 systemic fungal infections	178:212	~400,000 systemic fungal infections annually	178:221	Candida albicans is responsible for ~400,000 systemic fungal infections annually, with an associated mortality rate of 46-75%.
28066722	11	9	theme	wall	1966:1969	arg1	architecture					1971:1982	fungal cell wall architecture	1954:1982	fungal cell wall architecture	1954:1982	These data suggest that fungal cell wall architecture, more so than its crude composition, critically determines the ability of fungi to colonize the mammalian GI tract.
28066722	4	10	theme	competitive	610:620	arg1	fitness					622:628	the competitive fitness	606:628	the competitive fitness of Candida species in the mammalian GI tract	606:673	We hypothesized that the fungal cell wall would play an important role in determining the competitive fitness of Candida species in the mammalian GI tract.
28066722	7	11	theme	Similar	1040:1046	arg1	results					1048:1054	Similar results	1040:1054	Similar results	1040:1054	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	11	12	theme	fungal	1954:1959	arg1	wall					1966:1969	fungal cell wall	1954:1969	fungal cell wall architecture	1954:1982	These data suggest that fungal cell wall architecture, more so than its crude composition, critically determines the ability of fungi to colonize the mammalian GI tract.
28066722	0	13	theme	Gastrointestinal	118:133	arg1	Tract					135:139	the Mouse Gastrointestinal Tract	108:139	the Mouse Gastrointestinal Tract	108:139	β-glucan Exposure on the Fungal Cell Wall Tightly Correlates with Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract.
28066722	5	14	theme	mouse	814:818	arg1	tract					823:827	the mouse GI tract	810:827	the mouse GI tract via quantitative competition assays	810:863	To test this hypothesis, we generated a systematic collection of isogenic C. albicans cell wall mutants and measured their fitness in the mouse GI tract via quantitative competition assays.
28066722	12	15	theme	promising	2236:2244	arg1	target					2246:2251	a promising target	2234:2251	a promising target to reduce fungal colonization in patients at risks of systemic candidiasis	2234:2326	In particular, recognition of exposed β-glucan by Dectin-1 receptor appears to severely limit Candida GI fitness and hence represents a promising target to reduce fungal colonization in patients at risks of systemic candidiasis.
28066722	4	16	theme	important	576:584	arg1	role					586:589	an important role	573:589	an important role	573:589	We hypothesized that the fungal cell wall would play an important role in determining the competitive fitness of Candida species in the mammalian GI tract.
28066722	10	17	theme	fitness	1899:1905	arg1	predictive					1873:1882	predictive	1873:1882	predictive	1873:1882	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	6	18	theme	competitive	895:905	arg1	fitness					907:913	competitive fitness	895:913	competitive fitness	895:913	Whereas a large variation in competitive fitness was found among mutants, no correlation was observed between GI fitness and total levels of individual cell wall components.
28066722	12	19	theme	GI	2202:2203	arg1	fitness					2205:2211	Candida GI fitness	2194:2211	Candida GI fitness	2194:2211	In particular, recognition of exposed β-glucan by Dectin-1 receptor appears to severely limit Candida GI fitness and hence represents a promising target to reduce fungal colonization in patients at risks of systemic candidiasis.
28066722	8	20	theme	phenotypic	1340:1349	arg1	profiling					1351:1359	an extensive quantitative phenotypic profiling	1314:1359	an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness	1314:1444	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	9	21	theme	Dectin-1	1683:1690	arg1	receptor					1692:1699	the Dectin-1 receptor	1679:1699	the Dectin-1 receptor	1679:1699	The most significant association with GI fitness was found to be the strength of signaling through the Dectin-1 receptor.
28066722	4	22	theme	GI	666:667	arg1	tract					669:673	the mammalian GI tract	652:673	the mammalian GI tract	652:673	We hypothesized that the fungal cell wall would play an important role in determining the competitive fitness of Candida species in the mammalian GI tract.
28066722	10	23	theme	GI	1920:1921	arg1	tract					1923:1927	the mouse GI tract	1910:1927	the mouse GI tract	1910:1927	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	10	24	theme	exposed	1754:1760	arg1	β-glucan					1762:1769	exposed β-glucan	1754:1769	exposed β-glucan	1754:1769	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	1	25	theme	Candida	142:148	arg1	albicans					150:157	Candida albicans	142:157	Candida albicans	142:157	Candida albicans is responsible for ~400,000 systemic fungal infections annually, with an associated mortality rate of 46-75%.
28066722	6	26	theme	GI	976:977	arg1	fitness					979:985	GI fitness	976:985	GI fitness	976:985	Whereas a large variation in competitive fitness was found among mutants, no correlation was observed between GI fitness and total levels of individual cell wall components.
28066722	9	27	with	association	1601:1611	arg1	fitness					1621:1627	GI fitness	1618:1627	GI fitness	1618:1627	The most significant association with GI fitness was found to be the strength of signaling through the Dectin-1 receptor.
28066722	8	28	theme	Candida	1283:1289	arg1	strains					1291:1297	Candida strains	1283:1297	Candida strains	1283:1297	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	0	29	theme	Competitive	66:76	arg1	Fitness					78:84	Competitive Fitness	66:84	Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract	66:139	β-glucan Exposure on the Fungal Cell Wall Tightly Correlates with Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract.
28066722	7	30	theme	components	1171:1180	arg1	components					1171:1180	individual cell wall components	1150:1180	individual cell wall components	1150:1180	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	7	30	theme	components	1171:1180	arg1	amounts					1139:1145	total amounts	1133:1145	total amounts of individual cell wall components	1133:1180	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	8	31	dep	in	1561:1562	arg1	vivo					1564:1567	vivo	1564:1567	vivo	1564:1567	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	12	32	theme	β-glucan	2138:2145	arg1	recognition					2115:2125	recognition	2115:2125	recognition of exposed β-glucan by Dectin-1 receptor	2115:2166	In particular, recognition of exposed β-glucan by Dectin-1 receptor appears to severely limit Candida GI fitness and hence represents a promising target to reduce fungal colonization in patients at risks of systemic candidiasis.
28066722	2	33	theme	major	376:380	arg1	source					382:387	a major source	374:387	a major source of systemic fungal infections	374:417	The human gastrointestinal (GI) tract represents the largest natural reservoir of Candida species and is a major source of systemic fungal infections.
28066722	7	34	theme	cell	1161:1164	arg1	components					1171:1180	individual cell wall components	1150:1180	individual cell wall components	1150:1180	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	0	35	theme	Cell	32:35	arg1	Wall					37:40	the Fungal Cell Wall	21:40	the Fungal Cell Wall	21:40	β-glucan Exposure on the Fungal Cell Wall Tightly Correlates with Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract.
28066722	4	36	theme	fungal	545:550	arg1	wall					557:560	the fungal cell wall	541:560	the fungal cell wall	541:560	We hypothesized that the fungal cell wall would play an important role in determining the competitive fitness of Candida species in the mammalian GI tract.
28066722	5	37	theme	systematic	716:725	arg1	collection					727:736	a systematic collection	714:736	a systematic collection of isogenic C. albicans cell wall mutants	714:778	To test this hypothesis, we generated a systematic collection of isogenic C. albicans cell wall mutants and measured their fitness in the mouse GI tract via quantitative competition assays.
28066722	8	38	theme	extensive	1317:1325	arg1	profiling					1351:1359	an extensive quantitative phenotypic profiling	1314:1359	an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness	1314:1444	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	0	39	theme	β-glucan	0:7	arg1	Exposure					9:16	β-glucan Exposure	0:16	β-glucan Exposure on the Fungal Cell Wall	0:40	β-glucan Exposure on the Fungal Cell Wall Tightly Correlates with Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract.
28066722	6	40	theme	wall	1023:1026	arg1	components					1028:1037	individual cell wall components	1007:1037	individual cell wall components	1007:1037	Whereas a large variation in competitive fitness was found among mutants, no correlation was observed between GI fitness and total levels of individual cell wall components.
28066722	2	41	dep	natural	330:336	arg1	largest					322:328	largest	322:328	largest	322:328	The human gastrointestinal (GI) tract represents the largest natural reservoir of Candida species and is a major source of systemic fungal infections.
28066722	2	42	theme	fungal	401:406	arg1	infections					408:417	systemic fungal infections	392:417	systemic fungal infections	392:417	The human gastrointestinal (GI) tract represents the largest natural reservoir of Candida species and is a major source of systemic fungal infections.
28066722	7	43	theme	total	1133:1137	arg1	components					1171:1180	individual cell wall components	1150:1180	individual cell wall components	1150:1180	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	7	43	theme	total	1133:1137	arg1	amounts					1139:1145	total amounts	1133:1145	total amounts of individual cell wall components	1133:1180	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	6	44	theme	individual	1007:1016	arg1	components					1028:1037	individual cell wall components	1007:1037	individual cell wall components	1007:1037	Whereas a large variation in competitive fitness was found among mutants, no correlation was observed between GI fitness and total levels of individual cell wall components.
28066722	11	45	theme	GI	2090:2091	arg1	tract					2093:2097	the mammalian GI tract	2076:2097	the mammalian GI tract	2076:2097	These data suggest that fungal cell wall architecture, more so than its crude composition, critically determines the ability of fungi to colonize the mammalian GI tract.
28066722	8	46	theme	GI-mimicking	1500:1511	arg1	environments					1527:1538	GI-mimicking and stressful environments	1500:1538	GI-mimicking and stressful environments	1500:1538	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	10	47	theme	fungal	1789:1794	arg1	cells					1796:1800	fungal cells	1789:1800	fungal cells	1789:1800	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	0	48	theme	Fungal	25:30	arg1	Wall					37:40	the Fungal Cell Wall	21:40	the Fungal Cell Wall	21:40	β-glucan Exposure on the Fungal Cell Wall Tightly Correlates with Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract.
28066722	8	49	theme	stressful	1517:1525	arg1	environments					1527:1538	GI-mimicking and stressful environments	1500:1538	GI-mimicking and stressful environments	1500:1538	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	2	50	theme	natural	330:336	arg1	reservoir					338:346	the largest natural reservoir	318:346	the largest natural reservoir of Candida species	318:365	The human gastrointestinal (GI) tract represents the largest natural reservoir of Candida species and is a major source of systemic fungal infections.
28066722	5	51	theme	wall	767:770	arg1	mutants					772:778	isogenic C. albicans cell wall mutants	741:778	isogenic C. albicans cell wall mutants	741:778	To test this hypothesis, we generated a systematic collection of isogenic C. albicans cell wall mutants and measured their fitness in the mouse GI tract via quantitative competition assays.
28066722	12	52	theme	candidiasis	2316:2326	arg1	risks					2298:2302	risks	2298:2302	risks of systemic candidiasis	2298:2326	In particular, recognition of exposed β-glucan by Dectin-1 receptor appears to severely limit Candida GI fitness and hence represents a promising target to reduce fungal colonization in patients at risks of systemic candidiasis.
28066722	11	53	theme	fungi	2058:2062	arg1	ability					2047:2053	the ability	2043:2053	the ability of fungi to colonize the mammalian GI tract	2043:2097	These data suggest that fungal cell wall architecture, more so than its crude composition, critically determines the ability of fungi to colonize the mammalian GI tract.
28066722	3	54	theme	GI	454:455	arg1	colonization					457:468	GI colonization	454:468	GI colonization by Candida species	454:487	However, the factors that control GI colonization by Candida species are not completely understood.
28066722	8	55	theme	in	1548:1549	arg1	virulence					1569:1577	in vitro and in vivo virulence	1548:1577	in vitro and in vivo virulence	1548:1577	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	2	56	theme	species	359:365	arg1	reservoir					338:346	the largest natural reservoir	318:346	the largest natural reservoir of Candida species	318:365	The human gastrointestinal (GI) tract represents the largest natural reservoir of Candida species and is a major source of systemic fungal infections.
28066722	1	57	theme	associated	232:241	arg1	rate					253:256	an associated mortality rate	229:256	an associated mortality rate of 46-75%	229:266	Candida albicans is responsible for ~400,000 systemic fungal infections annually, with an associated mortality rate of 46-75%.
28066722	7	58	theme	Candida	1100:1106	arg1	species					1108:1114	distantly-related Candida species	1082:1114	distantly-related Candida species	1082:1114	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	10	59	theme	β-glucan	1841:1848	arg1	levels					1850:1855	total β-glucan levels	1835:1855	total β-glucan levels	1835:1855	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	6	60	theme	large	876:880	arg1	variation					882:890	a large variation	874:890	a large variation in competitive fitness	874:913	Whereas a large variation in competitive fitness was found among mutants, no correlation was observed between GI fitness and total levels of individual cell wall components.
28066722	9	61	theme	significant	1589:1599	arg1	association					1601:1611	The most significant association	1580:1611	The most significant association with GI fitness	1580:1627	The most significant association with GI fitness was found to be the strength of signaling through the Dectin-1 receptor.
28066722	9	61	theme	significant	1589:1599	arg1	strength					1649:1656	the strength	1645:1656	the strength of signaling through the Dectin-1 receptor	1645:1699	The most significant association with GI fitness was found to be the strength of signaling through the Dectin-1 receptor.
28066722	2	62	theme	gastrointestinal	279:294	arg1	tract					301:305	The human gastrointestinal (GI) tract	269:305	The human gastrointestinal (GI) tract	269:305	The human gastrointestinal (GI) tract represents the largest natural reservoir of Candida species and is a major source of systemic fungal infections.
28066722	11	63	theme	crude	2002:2006	arg1	composition					2008:2018	its crude composition	1998:2018	its crude composition	1998:2018	These data suggest that fungal cell wall architecture, more so than its crude composition, critically determines the ability of fungi to colonize the mammalian GI tract.
28066722	12	64	theme	fungal	2263:2268	arg1	colonization					2270:2281	fungal colonization	2263:2281	fungal colonization in patients	2263:2293	In particular, recognition of exposed β-glucan by Dectin-1 receptor appears to severely limit Candida GI fitness and hence represents a promising target to reduce fungal colonization in patients at risks of systemic candidiasis.
28066722	12	65	from	colonization	2270:2281	arg1	patients					2286:2293	patients	2286:2293	patients	2286:2293	In particular, recognition of exposed β-glucan by Dectin-1 receptor appears to severely limit Candida GI fitness and hence represents a promising target to reduce fungal colonization in patients at risks of systemic candidiasis.
28066722	5	66	theme	GI	820:821	arg1	tract					823:827	the mouse GI tract	810:827	the mouse GI tract via quantitative competition assays	810:863	To test this hypothesis, we generated a systematic collection of isogenic C. albicans cell wall mutants and measured their fitness in the mouse GI tract via quantitative competition assays.
28066722	7	67	theme	species	1108:1114	arg1	set					1075:1077	a set	1073:1077	a set of distantly-related Candida species	1073:1114	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	7	67	theme	species	1108:1114	arg1	species					1108:1114	distantly-related Candida species	1082:1114	distantly-related Candida species	1082:1114	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	4	68	theme	Candida	633:639	arg1	species					641:647	Candida species	633:647	Candida species	633:647	We hypothesized that the fungal cell wall would play an important role in determining the competitive fitness of Candida species in the mammalian GI tract.
28066722	5	69	theme	isogenic	741:748	arg1	mutants					772:778	isogenic C. albicans cell wall mutants	741:778	isogenic C. albicans cell wall mutants	741:778	To test this hypothesis, we generated a systematic collection of isogenic C. albicans cell wall mutants and measured their fitness in the mouse GI tract via quantitative competition assays.
28066722	8	70	from	profiling	1351:1359	arg1	search					1364:1369	search	1364:1369	search for features that might be responsible for their differences in GI fitness	1364:1444	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	12	71	theme	Candida	2194:2200	arg1	fitness					2205:2211	Candida GI fitness	2194:2211	Candida GI fitness	2194:2211	In particular, recognition of exposed β-glucan by Dectin-1 receptor appears to severely limit Candida GI fitness and hence represents a promising target to reduce fungal colonization in patients at risks of systemic candidiasis.
28066722	0	72	theme	Mouse	112:116	arg1	Tract					135:139	the Mouse Gastrointestinal Tract	108:139	the Mouse Gastrointestinal Tract	108:139	β-glucan Exposure on the Fungal Cell Wall Tightly Correlates with Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract.
28066722	2	73	theme	GI	297:298	arg1	tract					301:305	The human gastrointestinal (GI) tract	269:305	The human gastrointestinal (GI) tract	269:305	The human gastrointestinal (GI) tract represents the largest natural reservoir of Candida species and is a major source of systemic fungal infections.
28066722	4	74	from	fitness	622:628	arg1	tract					669:673	the mammalian GI tract	652:673	the mammalian GI tract	652:673	We hypothesized that the fungal cell wall would play an important role in determining the competitive fitness of Candida species in the mammalian GI tract.
28066722	1	75	theme	fungal	196:201	arg1	infections					203:212	~400,000 systemic fungal infections	178:212	~400,000 systemic fungal infections annually	178:221	Candida albicans is responsible for ~400,000 systemic fungal infections annually, with an associated mortality rate of 46-75%.
28066722	0	76	from	Fitness	78:84	arg1	Tract					135:139	the Mouse Gastrointestinal Tract	108:139	the Mouse Gastrointestinal Tract	108:139	β-glucan Exposure on the Fungal Cell Wall Tightly Correlates with Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract.
28066722	5	77	theme	quantitative	833:844	arg1	assays					858:863	quantitative competition assays	833:863	quantitative competition assays	833:863	To test this hypothesis, we generated a systematic collection of isogenic C. albicans cell wall mutants and measured their fitness in the mouse GI tract via quantitative competition assays.
28066722	11	78	theme	cell	1961:1964	arg1	wall					1966:1969	fungal cell wall	1954:1969	fungal cell wall architecture	1954:1982	These data suggest that fungal cell wall architecture, more so than its crude composition, critically determines the ability of fungi to colonize the mammalian GI tract.
28066722	7	79	theme	wall	1166:1169	arg1	components					1171:1180	individual cell wall components	1150:1180	individual cell wall components	1150:1180	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	9	80	theme	signaling	1661:1669	arg1	association					1601:1611	The most significant association	1580:1611	The most significant association with GI fitness	1580:1627	The most significant association with GI fitness was found to be the strength of signaling through the Dectin-1 receptor.
28066722	9	80	theme	signaling	1661:1669	arg1	strength					1649:1656	the strength	1645:1656	the strength of signaling through the Dectin-1 receptor	1645:1699	The most significant association with GI fitness was found to be the strength of signaling through the Dectin-1 receptor.
28066722	8	81	theme	GI	1435:1436	arg1	fitness					1438:1444	GI fitness	1435:1444	GI fitness	1435:1444	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	10	82	theme	competitive	1887:1897	arg1	fitness					1899:1905	competitive fitness	1887:1905	competitive fitness in the mouse GI tract	1887:1927	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	10	83	theme	quantitative	1710:1721	arg1	assay					1723:1727	a quantitative assay	1708:1727	a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells	1708:1800	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	6	84	theme	total	991:995	arg1	levels					997:1002	total levels	991:1002	total levels of individual cell wall components	991:1037	Whereas a large variation in competitive fitness was found among mutants, no correlation was observed between GI fitness and total levels of individual cell wall components.
28066722	8	85	from	differences	1420:1430	arg1	fitness					1438:1444	GI fitness	1435:1444	GI fitness	1435:1444	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	10	86	theme	β-glucan	1762:1769	arg1	amount					1744:1749	the amount	1740:1749	the amount of exposed β-glucan on the surface of fungal cells	1740:1800	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	10	86	theme	β-glucan	1762:1769	arg1	β-glucan					1762:1769	exposed β-glucan	1754:1769	exposed β-glucan	1754:1769	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	5	87	theme	competition	846:856	arg1	assays					858:863	quantitative competition assays	833:863	quantitative competition assays	833:863	To test this hypothesis, we generated a systematic collection of isogenic C. albicans cell wall mutants and measured their fitness in the mouse GI tract via quantitative competition assays.
28066722	4	88	theme	mammalian	656:664	arg1	tract					669:673	the mammalian GI tract	652:673	the mammalian GI tract	652:673	We hypothesized that the fungal cell wall would play an important role in determining the competitive fitness of Candida species in the mammalian GI tract.
28066722	10	89	theme	mouse	1914:1918	arg1	tract					1923:1927	the mouse GI tract	1910:1927	the mouse GI tract	1910:1927	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	0	90	from	Exposure	9:16	arg1	Wall					37:40	the Fungal Cell Wall	21:40	the Fungal Cell Wall	21:40	β-glucan Exposure on the Fungal Cell Wall Tightly Correlates with Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract.
28066722	0	91	theme	Candida	89:95	arg1	Species					97:103	Candida Species	89:103	Candida Species	89:103	β-glucan Exposure on the Fungal Cell Wall Tightly Correlates with Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract.
28066722	12	92	theme	Dectin-1	2150:2157	arg1	receptor					2159:2166	Dectin-1 receptor	2150:2166	Dectin-1 receptor	2150:2166	In particular, recognition of exposed β-glucan by Dectin-1 receptor appears to severely limit Candida GI fitness and hence represents a promising target to reduce fungal colonization in patients at risks of systemic candidiasis.
28066722	8	93	theme	strains	1291:1297	arg1	collection					1269:1278	this collection	1264:1278	this collection of Candida strains and species	1264:1309	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	7	94	theme	individual	1150:1159	arg1	components					1171:1180	individual cell wall components	1150:1180	individual cell wall components	1150:1180	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	10	95	from	fitness	1899:1905	arg1	tract					1923:1927	the mouse GI tract	1910:1927	the mouse GI tract	1910:1927	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	8	96	theme	species	1303:1309	arg1	collection					1269:1278	this collection	1264:1278	this collection of Candida strains and species	1264:1309	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	5	97	theme	mutants	772:778	arg1	collection					727:736	a systematic collection	714:736	a systematic collection of isogenic C. albicans cell wall mutants	714:778	To test this hypothesis, we generated a systematic collection of isogenic C. albicans cell wall mutants and measured their fitness in the mouse GI tract via quantitative competition assays.
28066722	8	98	dep	in	1548:1549	arg1	vitro					1551:1555	vitro	1551:1555	vitro	1551:1555	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	10	99	from	amount	1744:1749	arg1	surface					1778:1784	the surface	1774:1784	the surface of fungal cells	1774:1800	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	6	100	theme	components	1028:1037	arg1	fitness					979:985	GI fitness	976:985	GI fitness	976:985	Whereas a large variation in competitive fitness was found among mutants, no correlation was observed between GI fitness and total levels of individual cell wall components.
28066722	6	100	theme	components	1028:1037	arg1	levels					997:1002	total levels	991:1002	total levels of individual cell wall components	991:1037	Whereas a large variation in competitive fitness was found among mutants, no correlation was observed between GI fitness and total levels of individual cell wall components.
28066722	6	101	theme	cell	1018:1021	arg1	components					1028:1037	individual cell wall components	1007:1037	individual cell wall components	1007:1037	Whereas a large variation in competitive fitness was found among mutants, no correlation was observed between GI fitness and total levels of individual cell wall components.
28066722	2	102	theme	infections	408:417	arg1	source					382:387	a major source	374:387	a major source of systemic fungal infections	374:417	The human gastrointestinal (GI) tract represents the largest natural reservoir of Candida species and is a major source of systemic fungal infections.
28066722	4	103	theme	cell	552:555	arg1	wall					557:560	the fungal cell wall	541:560	the fungal cell wall	541:560	We hypothesized that the fungal cell wall would play an important role in determining the competitive fitness of Candida species in the mammalian GI tract.
28066722	10	104	theme	cells	1796:1800	arg1	surface					1778:1784	the surface	1774:1784	the surface of fungal cells	1774:1800	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	8	105	theme	quantitative	1327:1338	arg1	profiling					1351:1359	an extensive quantitative phenotypic profiling	1314:1359	an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness	1314:1444	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	2	106	theme	systemic	392:399	arg1	infections					408:417	systemic fungal infections	392:417	systemic fungal infections	392:417	The human gastrointestinal (GI) tract represents the largest natural reservoir of Candida species and is a major source of systemic fungal infections.
28066722	5	107	theme	C.	750:751	arg1	mutants					772:778	isogenic C. albicans cell wall mutants	741:778	isogenic C. albicans cell wall mutants	741:778	To test this hypothesis, we generated a systematic collection of isogenic C. albicans cell wall mutants and measured their fitness in the mouse GI tract via quantitative competition assays.
28066722	12	108	theme	systemic	2307:2314	arg1	candidiasis					2316:2326	systemic candidiasis	2307:2326	systemic candidiasis	2307:2326	In particular, recognition of exposed β-glucan by Dectin-1 receptor appears to severely limit Candida GI fitness and hence represents a promising target to reduce fungal colonization in patients at risks of systemic candidiasis.
28066722	0	109	theme	Species	97:103	arg1	Fitness					78:84	Competitive Fitness	66:84	Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract	66:139	β-glucan Exposure on the Fungal Cell Wall Tightly Correlates with Competitive Fitness of Candida Species in the Mouse Gastrointestinal Tract.
28066722	5	110	theme	cell	762:765	arg1	mutants					772:778	isogenic C. albicans cell wall mutants	741:778	isogenic C. albicans cell wall mutants	741:778	To test this hypothesis, we generated a systematic collection of isogenic C. albicans cell wall mutants and measured their fitness in the mouse GI tract via quantitative competition assays.
28066722	7	111	theme	GI	1236:1237	arg1	tract					1239:1243	the GI tract	1232:1243	the GI tract	1232:1243	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	11	112	theme	mammalian	2080:2088	arg1	tract					2093:2097	the mammalian GI tract	2076:2097	the mammalian GI tract	2076:2097	These data suggest that fungal cell wall architecture, more so than its crude composition, critically determines the ability of fungi to colonize the mammalian GI tract.
28066722	3	113	theme	Candida	473:479	arg1	species					481:487	Candida species	473:487	Candida species	473:487	However, the factors that control GI colonization by Candida species are not completely understood.
28066722	1	114	with	responsible	162:172	arg1	rate					253:256	an associated mortality rate	229:256	an associated mortality rate of 46-75%	229:266	Candida albicans is responsible for ~400,000 systemic fungal infections annually, with an associated mortality rate of 46-75%.
28066722	12	115	theme	exposed	2130:2136	arg1	β-glucan					2138:2145	exposed β-glucan	2130:2145	exposed β-glucan	2130:2145	In particular, recognition of exposed β-glucan by Dectin-1 receptor appears to severely limit Candida GI fitness and hence represents a promising target to reduce fungal colonization in patients at risks of systemic candidiasis.
28066722	7	116	theme	fungi	1214:1218	arg1	ability					1203:1209	the ability	1199:1209	the ability of fungi to colonize the GI tract	1199:1243	Similar results were obtained in a set of distantly-related Candida species, suggesting that total amounts of individual cell wall components do not determine the ability of fungi to colonize the GI tract.
28066722	8	117	theme	in	1561:1562	arg1	virulence					1569:1577	in vitro and in vivo virulence	1548:1577	in vitro and in vivo virulence	1548:1577	We then subjected this collection of Candida strains and species to an extensive quantitative phenotypic profiling in search for features that might be responsible for their differences in GI fitness, but found no association with the ability to grow in GI-mimicking and stressful environments or with in vitro and in vivo virulence.
28066722	10	118	theme	total	1835:1839	arg1	levels					1850:1855	total β-glucan levels	1835:1855	total β-glucan levels	1835:1855	Using a quantitative assay to measure the amount of exposed β-glucan on the surface of fungal cells, we found this parameter, unlike total β-glucan levels, to be strongly predictive of competitive fitness in the mouse GI tract.
28066722	2	119	theme	Candida	351:357	arg1	species					359:365	Candida species	351:365	Candida species	351:365	The human gastrointestinal (GI) tract represents the largest natural reservoir of Candida species and is a major source of systemic fungal infections.
28066722	1	120	theme	mortality	243:251	arg1	rate					253:256	an associated mortality rate	229:256	an associated mortality rate of 46-75%	229:266	Candida albicans is responsible for ~400,000 systemic fungal infections annually, with an associated mortality rate of 46-75%.
26632656	6	0	theme	guanidine-HCl	689:701	arg1	buffer					714:719	guanidine-HCl extraction buffer	689:719	guanidine-HCl extraction buffer	689:719	The addition of surfactant to guanidine-HCl extraction buffer improved protein solubility.
26632656	2	1	contain	have	262:265	arg1	studies					209:215	Most prior studies	198:215	Most prior studies defining the zonal distribution of cartilage	198:260	Most prior studies defining the zonal distribution of cartilage have extracted proteins with guanidine-HCl.
26632656	2	1	contain	have	262:265	arg2	proteins					277:284	extracted proteins	267:284	extracted proteins	267:284	Most prior studies defining the zonal distribution of cartilage have extracted proteins with guanidine-HCl.
26632656	11	2	theme	joint	1436:1440	arg1	biomarkers					1451:1460	the joint specific biomarkers	1432:1460	the joint specific biomarkers	1432:1460	The results of this study led to the identifications of the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers.
26632656	4	3	theme	polysaccharide	422:435	arg1	molecules					437:445	anionic polysaccharide molecules	414:445	anionic polysaccharide molecules extracted from cartilage	414:470	In addition, the high abundance of anionic polysaccharide molecules extracted from cartilage adversely affects the chromatographic separation.
26632656	10	4	theme	proteomic	1287:1295	arg1	analyses					1297:1304	quantitative and qualitative proteomic analyses	1258:1304	quantitative and qualitative proteomic analyses	1258:1304	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	2	5	theme	Most	198:201	arg1	studies					209:215	Most prior studies	198:215	Most prior studies defining the zonal distribution of cartilage	198:260	Most prior studies defining the zonal distribution of cartilage have extracted proteins with guanidine-HCl.
26632656	5	6	attach	removing	565:572	arg2	method					554:559	a method	552:559	a method for removing chondrocytes from cartilage sections with minimal extracellular matrix protein loss	552:656	In this study, we established a method for removing chondrocytes from cartilage sections with minimal extracellular matrix protein loss.
26632656	5	6	attach	removing	565:572	arg1	sections					602:609	cartilage sections	592:609	cartilage sections with minimal extracellular matrix protein loss	592:656	In this study, we established a method for removing chondrocytes from cartilage sections with minimal extracellular matrix protein loss.
26632656	4	7	theme	anionic	414:420	arg1	molecules					437:445	anionic polysaccharide molecules	414:445	anionic polysaccharide molecules extracted from cartilage	414:470	In addition, the high abundance of anionic polysaccharide molecules extracted from cartilage adversely affects the chromatographic separation.
26632656	6	8	theme	surfactant	675:684	arg1	addition					663:670	The addition	659:670	The addition of surfactant to guanidine-HCl extraction buffer	659:719	The addition of surfactant to guanidine-HCl extraction buffer improved protein solubility.
26632656	10	9	theme	qualitative	1275:1285	arg1	analyses					1297:1304	quantitative and qualitative proteomic analyses	1258:1304	quantitative and qualitative proteomic analyses	1258:1304	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	11	10	theme	osteoarthritis	1391:1404	arg1	biomarkers					1377:1386	the potential biomarkers	1363:1386	the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers	1363:1460	The results of this study led to the identifications of the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers.
26632656	1	11	theme	Articular	66:74	arg1	cartilage					76:84	Articular cartilage	66:84	Articular cartilage	66:84	Articular cartilage consists of chondrocytes and two major components, a collagen-rich framework and highly abundant proteoglycans.
26632656	11	12	theme	progression	1415:1425	arg1	biomarkers					1377:1386	the potential biomarkers	1363:1386	the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers	1363:1460	The results of this study led to the identifications of the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers.
26632656	5	13	with	sections	602:609	arg1	loss					653:656	minimal extracellular matrix protein loss	616:656	minimal extracellular matrix protein loss	616:656	In this study, we established a method for removing chondrocytes from cartilage sections with minimal extracellular matrix protein loss.
26632656	8	14	dep	in	882:883	arg1	situ					885:888	situ	885:888	situ	885:888	Almost four-times more collagen peptides were extracted by the in situ trypsin digestion method.
26632656	4	15	theme	molecules	437:445	arg1	abundance					401:409	the high abundance	392:409	the high abundance of anionic polysaccharide molecules extracted from cartilage	392:470	In addition, the high abundance of anionic polysaccharide molecules extracted from cartilage adversely affects the chromatographic separation.
26632656	1	16	theme	abundant	174:181	arg1	proteoglycans					183:195	highly abundant proteoglycans	167:195	highly abundant proteoglycans	167:195	Articular cartilage consists of chondrocytes and two major components, a collagen-rich framework and highly abundant proteoglycans.
26632656	1	16	theme	abundant	174:181	arg1	components					125:134	two major components	115:134	two major components	115:134	Articular cartilage consists of chondrocytes and two major components, a collagen-rich framework and highly abundant proteoglycans.
26632656	10	17	from	states	1183:1188	arg1	sections					1063:1070	extract cartilage sections	1045:1070	extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic)	1045:1217	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	10	18	theme	disease	1175:1181	arg1	states					1183:1188	disease states	1175:1188	disease states (healthy and osteoarthritic)	1175:1217	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	8	19	theme	digestion	898:906	arg1	method					908:913	the in situ trypsin digestion method	878:913	the in situ trypsin digestion method	878:913	Almost four-times more collagen peptides were extracted by the in situ trypsin digestion method.
26632656	4	20	theme	high	396:399	arg1	abundance					401:409	the high abundance	392:409	the high abundance of anionic polysaccharide molecules extracted from cartilage	392:470	In addition, the high abundance of anionic polysaccharide molecules extracted from cartilage adversely affects the chromatographic separation.
26632656	10	21	dep	states	1183:1188	arg1	osteoarthritic					1203:1216	osteoarthritic	1203:1216	osteoarthritic	1203:1216	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	10	21	dep	states	1183:1188	arg1	healthy					1191:1197	healthy	1191:1197	healthy	1191:1197	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	11	22	theme	potential	1367:1375	arg1	biomarkers					1377:1386	the potential biomarkers	1363:1386	the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers	1363:1460	The results of this study led to the identifications of the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers.
26632656	5	23	theme	matrix	638:643	arg1	loss					653:656	minimal extracellular matrix protein loss	616:656	minimal extracellular matrix protein loss	616:656	In this study, we established a method for removing chondrocytes from cartilage sections with minimal extracellular matrix protein loss.
26632656	10	24	from	layers	1097:1102	arg1	sections					1063:1070	extract cartilage sections	1045:1070	extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic)	1045:1217	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	10	25	theme	joint	1143:1147	arg1	types					1149:1153	joint types	1143:1153	joint types (knee and hip)	1143:1168	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	10	25	theme	joint	1143:1147	arg1	hip					1165:1167	hip	1165:1167	hip	1165:1167	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	10	25	theme	joint	1143:1147	arg1	knee					1156:1159	knee	1156:1159	knee	1156:1159	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	5	26	theme	protein	645:651	arg1	loss					653:656	minimal extracellular matrix protein loss	616:656	minimal extracellular matrix protein loss	616:656	In this study, we established a method for removing chondrocytes from cartilage sections with minimal extracellular matrix protein loss.
26632656	10	27	theme	different	1077:1085	arg1	layers					1097:1102	different cartilage layers	1077:1102	different cartilage layers (superficial, intermediate, and deep)	1077:1140	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	10	28	theme	cartilage	1053:1061	arg1	sections					1063:1070	extract cartilage sections	1045:1070	extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic)	1045:1217	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	0	29	theme	Molecular	16:24	arg1	Composition					26:36	the Molecular Composition	12:36	the Molecular Composition of Cartilage by Proteomics	12:63	Elucidating the Molecular Composition of Cartilage by Proteomics.
26632656	8	30	theme	more	837:840	arg1	peptides					851:858	Almost four-times more collagen peptides	819:858	Almost four-times more collagen peptides	819:858	Almost four-times more collagen peptides were extracted by the in situ trypsin digestion method.
26632656	4	31	theme	chromatographic	494:508	arg1	separation					510:519	the chromatographic separation	490:519	the chromatographic separation	490:519	In addition, the high abundance of anionic polysaccharide molecules extracted from cartilage adversely affects the chromatographic separation.
26632656	10	32	theme	extract	1045:1051	arg1	sections					1063:1070	extract cartilage sections	1045:1070	extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic)	1045:1217	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	10	33	theme	different	1014:1022	arg1	methods					1024:1030	These different methods	1008:1030	These different methods	1008:1030	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	0	34	theme	Cartilage	41:49	arg1	Composition					26:36	the Molecular Composition	12:36	the Molecular Composition of Cartilage by Proteomics	12:63	Elucidating the Molecular Composition of Cartilage by Proteomics.
26632656	8	35	theme	four-times	826:835	arg1	peptides					851:858	Almost four-times more collagen peptides	819:858	Almost four-times more collagen peptides	819:858	Almost four-times more collagen peptides were extracted by the in situ trypsin digestion method.
26632656	2	36	theme	cartilage	252:260	arg1	distribution					236:247	the zonal distribution	226:247	the zonal distribution of cartilage	226:260	Most prior studies defining the zonal distribution of cartilage have extracted proteins with guanidine-HCl.
26632656	10	37	used	used	1037:1040	arg2	methods					1024:1030	These different methods	1008:1030	These different methods	1008:1030	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	8	38	theme	trypsin	890:896	arg1	method					908:913	the in situ trypsin digestion method	878:913	the in situ trypsin digestion method	878:913	Almost four-times more collagen peptides were extracted by the in situ trypsin digestion method.
26632656	5	39	theme	cartilage	592:600	arg1	sections					602:609	cartilage sections	592:609	cartilage sections with minimal extracellular matrix protein loss	592:656	In this study, we established a method for removing chondrocytes from cartilage sections with minimal extracellular matrix protein loss.
26632656	10	40	dep	layers	1097:1102	arg1	intermediate					1118:1129	intermediate	1118:1129	intermediate	1118:1129	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	10	40	dep	layers	1097:1102	arg1	deep					1136:1139	deep	1136:1139	deep	1136:1139	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	10	40	dep	layers	1097:1102	arg1	superficial					1105:1115	superficial	1105:1115	superficial	1105:1115	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	11	41	theme	OA	1412:1413	arg1	progression					1415:1425	OA progression	1412:1425	OA progression	1412:1425	The results of this study led to the identifications of the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers.
26632656	8	42	theme	collagen	842:849	arg1	peptides					851:858	Almost four-times more collagen peptides	819:858	Almost four-times more collagen peptides	819:858	Almost four-times more collagen peptides were extracted by the in situ trypsin digestion method.
26632656	10	43	theme	cartilage	1087:1095	arg1	layers					1097:1102	different cartilage layers	1077:1102	different cartilage layers (superficial, intermediate, and deep)	1077:1140	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	1	44	theme	major	119:123	arg1	framework					153:161	a collagen-rich framework	137:161	a collagen-rich framework	137:161	Articular cartilage consists of chondrocytes and two major components, a collagen-rich framework and highly abundant proteoglycans.
26632656	1	44	theme	major	119:123	arg1	components					125:134	two major components	115:134	two major components	115:134	Articular cartilage consists of chondrocytes and two major components, a collagen-rich framework and highly abundant proteoglycans.
26632656	1	44	theme	major	119:123	arg1	proteoglycans					183:195	highly abundant proteoglycans	167:195	highly abundant proteoglycans	167:195	Articular cartilage consists of chondrocytes and two major components, a collagen-rich framework and highly abundant proteoglycans.
26632656	9	45	theme	guanidine-HCl	982:994	arg1	extraction					996:1005	the guanidine-HCl extraction	978:1005	the guanidine-HCl extraction	978:1005	However, as expected, proteoglycans were more abundant within the guanidine-HCl extraction.
26632656	2	46	theme	zonal	230:234	arg1	distribution					236:247	the zonal distribution	226:247	the zonal distribution of cartilage	226:260	Most prior studies defining the zonal distribution of cartilage have extracted proteins with guanidine-HCl.
26632656	5	47	theme	extracellular	624:636	arg1	loss					653:656	minimal extracellular matrix protein loss	616:656	minimal extracellular matrix protein loss	616:656	In this study, we established a method for removing chondrocytes from cartilage sections with minimal extracellular matrix protein loss.
26632656	10	48	from	types	1149:1153	arg1	sections					1063:1070	extract cartilage sections	1045:1070	extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic)	1045:1217	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	5	49	theme	minimal	616:622	arg1	loss					653:656	minimal extracellular matrix protein loss	616:656	minimal extracellular matrix protein loss	616:656	In this study, we established a method for removing chondrocytes from cartilage sections with minimal extracellular matrix protein loss.
26632656	6	50	theme	protein	730:736	arg1	solubility					738:747	protein solubility	730:747	protein solubility	730:747	The addition of surfactant to guanidine-HCl extraction buffer improved protein solubility.
26632656	10	51	theme	quantitative	1258:1269	arg1	analyses					1297:1304	quantitative and qualitative proteomic analyses	1258:1304	quantitative and qualitative proteomic analyses	1258:1304	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	11	52	theme	study	1327:1331	arg1	results					1311:1317	The results	1307:1317	The results of this study	1307:1331	The results of this study led to the identifications of the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers.
26632656	11	53	theme	specific	1442:1449	arg1	biomarkers					1451:1460	the joint specific biomarkers	1432:1460	the joint specific biomarkers	1432:1460	The results of this study led to the identifications of the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers.
26632656	2	54	theme	prior	203:207	arg1	studies					209:215	Most prior studies	198:215	Most prior studies defining the zonal distribution of cartilage	198:260	Most prior studies defining the zonal distribution of cartilage have extracted proteins with guanidine-HCl.
26632656	2	55	theme	extracted	267:275	arg1	proteins					277:284	extracted proteins	267:284	extracted proteins	267:284	Most prior studies defining the zonal distribution of cartilage have extracted proteins with guanidine-HCl.
26632656	1	56	theme	collagen-rich	139:151	arg1	framework					153:161	a collagen-rich framework	137:161	a collagen-rich framework	137:161	Articular cartilage consists of chondrocytes and two major components, a collagen-rich framework and highly abundant proteoglycans.
26632656	1	56	theme	collagen-rich	139:151	arg1	components					125:134	two major components	115:134	two major components	115:134	Articular cartilage consists of chondrocytes and two major components, a collagen-rich framework and highly abundant proteoglycans.
26632656	11	57	theme	biomarkers	1451:1460	arg1	biomarkers					1377:1386	the potential biomarkers	1363:1386	the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers	1363:1460	The results of this study led to the identifications of the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers.
26632656	11	58	theme	biomarkers	1377:1386	arg1	identifications					1344:1358	the identifications	1340:1358	the identifications of the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers	1340:1460	The results of this study led to the identifications of the potential biomarkers of osteoarthritis (OA), OA progression, and the joint specific biomarkers.
26632656	10	59	dep	types	1149:1153	arg1	types					1149:1153	joint types	1143:1153	joint types (knee and hip)	1143:1168	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	10	59	dep	types	1149:1153	arg1	hip					1165:1167	hip	1165:1167	hip	1165:1167	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	10	59	dep	types	1149:1153	arg1	knee					1156:1159	knee	1156:1159	knee	1156:1159	These different methods were used to extract cartilage sections from different cartilage layers (superficial, intermediate, and deep), joint types (knee and hip), and disease states (healthy and osteoarthritic), and the extractions were evaluated by quantitative and qualitative proteomic analyses.
26632656	6	60	theme	extraction	703:712	arg1	buffer					714:719	guanidine-HCl extraction buffer	689:719	guanidine-HCl extraction buffer	689:719	The addition of surfactant to guanidine-HCl extraction buffer improved protein solubility.
26632656	8	61	theme	in	882:883	arg1	method					908:913	the in situ trypsin digestion method	878:913	the in situ trypsin digestion method	878:913	Almost four-times more collagen peptides were extracted by the in situ trypsin digestion method.
25801069	0	0	theme	formulas	77:84	arg1	aspects					37:43	the nutritional aspects	21:43	the nutritional aspects of initial and follow-on infant formulas	21:84	[Expert consensus on the nutritional aspects of initial and follow-on infant formulas].
25801069	5	1	theme	criteria	672:679	arg1	consensus					681:689	a professional criteria consensus	657:689	a professional criteria consensus on nutritional aspects of infant formulas	657:731	MATERIAL AND METHODS A survey, using 62 items, stratified into 5 blocks, was performed by 48 panelists using the Delphi method to achieve a professional criteria consensus on nutritional aspects of infant formulas.
25801069	1	2	from	health	162:167	arg1	term					191:194	the short and long term	172:194	the short and long term	172:194	INTRODUCTION Infant feeding in the first months/years of life affects the health in the short and long term.
25801069	1	3	theme	life	145:148	arg1	months/years					129:140	the first months/years	119:140	the first months/years of life	119:148	INTRODUCTION Infant feeding in the first months/years of life affects the health in the short and long term.
25801069	0	4	theme	infant	70:75	arg1	formulas					77:84	initial and follow-on infant formulas	48:84	initial and follow-on infant formulas	48:84	[Expert consensus on the nutritional aspects of initial and follow-on infant formulas].
25801069	0	5	from	consensus	8:16	arg1	aspects					37:43	the nutritional aspects	21:43	the nutritional aspects of initial and follow-on infant formulas	21:84	[Expert consensus on the nutritional aspects of initial and follow-on infant formulas].
25801069	6	6	from	influence	901:909	arg1	development					929:939	body and brain development	914:939	development	929:939	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	6	6	from	influence	901:909	arg1	maturity					952:959	immune maturity	945:959	immune maturity	945:959	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	7	7	from	prebiotics	1108:1117	arg1	formulas					1129:1136	infant formulas	1122:1136	infant formulas	1122:1136	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	5	8	theme	Delphi	632:637	arg1	method					639:644	the Delphi method	628:644	the Delphi method	628:644	MATERIAL AND METHODS A survey, using 62 items, stratified into 5 blocks, was performed by 48 panelists using the Delphi method to achieve a professional criteria consensus on nutritional aspects of infant formulas.
25801069	8	9	theme	nutritional	1264:1274	arg1	quality					1276:1282	nutritional quality	1264:1282	nutritional quality of proteins	1264:1294	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	8	9	theme	nutritional	1264:1274	arg1	supplementation					1324:1338	taurine supplementation	1316:1338	taurine supplementation	1316:1338	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	8	9	theme	nutritional	1264:1274	arg1	use					1297:1299	use	1297:1299	use of thickeners	1297:1313	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	7	10	theme	immune	1163:1168	arg1	development					1182:1192	a correct cerebral, immune and somatic development	1143:1192	a correct cerebral, immune and somatic development	1143:1192	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	7	11	theme	vitamin	1093:1099	arg1	D					1101:1101	vitamin D	1093:1101	vitamin D	1093:1101	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	7	12	from	composition	1052:1062	arg1	formulas					1129:1136	infant formulas	1122:1136	infant formulas	1122:1136	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	4	13	theme	study	360:364	arg1	aim					348:350	The aim	344:350	The aim of this study	344:364	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	9	14	theme	More	1375:1378	arg1	studies					1380:1386	More studies	1375:1386	More studies	1375:1386	More studies are necessary to confirm these subjects.
25801069	6	15	theme	formulas	881:888	arg1	aspects					863:869	the nutritional aspects	847:869	the nutritional aspects of infant formulas	847:888	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	6	15	theme	formulas	881:888	arg1	influence					901:909	their influence	895:909	their influence in body and brain development and immune maturity	895:959	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	3	16	theme	infant	302:307	arg1	formulas					309:316	infant formulas	302:316	infant formulas	302:316	However, when breastfeeding is not possible, infant formulas are the best alternative.
25801069	3	16	theme	infant	302:307	arg1	alternative					331:341	the best alternative	322:341	the best alternative	322:341	However, when breastfeeding is not possible, infant formulas are the best alternative.
25801069	4	17	theme	formulas	408:415	arg1	role					383:386	the role	379:386	the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition	379:516	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	2	18	theme	many	242:245	arg1	benefits					247:254	its many benefits	238:254	its many benefits	238:254	Breastfeeding is the perfect food due to its many benefits.
25801069	5	19	theme	nutritional	694:704	arg1	aspects					706:712	nutritional aspects	694:712	nutritional aspects of infant formulas	694:731	MATERIAL AND METHODS A survey, using 62 items, stratified into 5 blocks, was performed by 48 panelists using the Delphi method to achieve a professional criteria consensus on nutritional aspects of infant formulas.
25801069	6	20	theme	items	782:786	arg1	%					773:773	64.6%	769:773	64.6% of the items	769:786	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	6	20	theme	items	782:786	arg1	items					782:786	the items	778:786	the items	778:786	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	4	21	from	gastroenterology	487:502	arg1	panel					460:464	a panel	458:464	a panel of experts in infant gastroenterology	458:502	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	8	22	dep	yet	1229:1231	arg1	not					1225:1227	not	1225:1227	not	1225:1227	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	5	23	theme	infant	717:722	arg1	formulas					724:731	infant formulas	717:731	infant formulas	717:731	MATERIAL AND METHODS A survey, using 62 items, stratified into 5 blocks, was performed by 48 panelists using the Delphi method to achieve a professional criteria consensus on nutritional aspects of infant formulas.
25801069	4	24	theme	infant	420:425	arg1	nutrition					427:435	infant nutrition	420:435	infant nutrition using the opinion of a panel of experts in infant gastroenterology	420:502	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	6	25	theme	infant	874:879	arg1	formulas					881:888	infant formulas	874:888	infant formulas	874:888	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	7	26	theme	somatic	1174:1180	arg1	development					1182:1192	a correct cerebral, immune and somatic development	1143:1192	a correct cerebral, immune and somatic development	1143:1192	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	0	27	theme	[Expert	0:6	arg1	consensus					8:16	[Expert consensus	0:16	[Expert consensus on the nutritional aspects of initial and follow-on infant formulas	0:84	[Expert consensus on the nutritional aspects of initial and follow-on infant formulas].
25801069	4	28	theme	infant	480:485	arg1	gastroenterology					487:502	infant gastroenterology	480:502	infant gastroenterology	480:502	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	1	29	from	feeding	108:114	arg1	months/years					129:140	the first months/years	119:140	the first months/years of life	119:148	INTRODUCTION Infant feeding in the first months/years of life affects the health in the short and long term.
25801069	8	30	theme	taurine	1316:1322	arg1	quality					1276:1282	nutritional quality	1264:1282	nutritional quality of proteins	1264:1294	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	8	30	theme	taurine	1316:1322	arg1	supplementation					1324:1338	taurine supplementation	1316:1338	taurine supplementation	1316:1338	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	3	31	theme	best	326:329	arg1	formulas					309:316	infant formulas	302:316	infant formulas	302:316	However, when breastfeeding is not possible, infant formulas are the best alternative.
25801069	3	31	theme	best	326:329	arg1	alternative					331:341	the best alternative	322:341	the best alternative	322:341	However, when breastfeeding is not possible, infant formulas are the best alternative.
25801069	5	32	theme	formulas	724:731	arg1	aspects					706:712	nutritional aspects	694:712	nutritional aspects of infant formulas	694:731	MATERIAL AND METHODS A survey, using 62 items, stratified into 5 blocks, was performed by 48 panelists using the Delphi method to achieve a professional criteria consensus on nutritional aspects of infant formulas.
25801069	6	33	from	aspects	863:869	arg1	development					929:939	body and brain development	914:939	development	929:939	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	6	33	from	aspects	863:869	arg1	maturity					952:959	immune maturity	945:959	immune maturity	945:959	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	1	34	theme	INTRODUCTION	88:99	arg1	feeding					108:114	INTRODUCTION Infant feeding	88:114	INTRODUCTION Infant feeding in the first months/years of life	88:148	INTRODUCTION Infant feeding in the first months/years of life affects the health in the short and long term.
25801069	1	35	theme	short	176:180	arg1	term					191:194	the short and long term	172:194	the short and long term	172:194	INTRODUCTION Infant feeding in the first months/years of life affects the health in the short and long term.
25801069	8	36	theme	thickeners	1304:1313	arg1	quality					1276:1282	nutritional quality	1264:1282	nutritional quality of proteins	1264:1294	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	8	36	theme	thickeners	1304:1313	arg1	use					1297:1299	use	1297:1299	use of thickeners	1297:1313	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	7	37	from	lactose	1075:1081	arg1	formulas					1129:1136	infant formulas	1122:1136	infant formulas	1122:1136	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	1	38	theme	Infant	101:106	arg1	feeding					108:114	INTRODUCTION Infant feeding	88:114	INTRODUCTION Infant feeding in the first months/years of life	88:148	INTRODUCTION Infant feeding in the first months/years of life affects the health in the short and long term.
25801069	6	39	dep	RESULTS	734:740	arg1	reached					758:764	reached	758:764	reached	758:764	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	4	40	from	experts	469:475	arg1	gastroenterology					487:502	infant gastroenterology	480:502	infant gastroenterology	480:502	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	6	41	theme	nutritional	851:861	arg1	aspects					863:869	the nutritional aspects	847:869	the nutritional aspects of infant formulas	847:888	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	7	42	theme	cerebral	1153:1160	arg1	development					1182:1192	a correct cerebral, immune and somatic development	1143:1192	a correct cerebral, immune and somatic development	1143:1192	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	1	43	theme	long	186:189	arg1	term					191:194	the short and long term	172:194	the short and long term	172:194	INTRODUCTION Infant feeding in the first months/years of life affects the health in the short and long term.
25801069	0	44	theme	nutritional	25:35	arg1	aspects					37:43	the nutritional aspects	21:43	the nutritional aspects of initial and follow-on infant formulas	21:84	[Expert consensus on the nutritional aspects of initial and follow-on infant formulas].
25801069	4	45	theme	panel	460:464	arg1	opinion					447:453	the opinion	443:453	the opinion of a panel of experts in infant gastroenterology	443:502	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	4	46	from	role	383:386	arg1	nutrition					508:516	nutrition	508:516	nutrition	508:516	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	4	46	from	role	383:386	arg1	nutrition					427:435	infant nutrition	420:435	infant nutrition using the opinion of a panel of experts in infant gastroenterology	420:502	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	4	47	theme	supplemented	395:406	arg1	formulas					408:415	the supplemented formulas	391:415	the supplemented formulas	391:415	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	6	48	theme	brain	923:927	arg1	development					929:939	body and brain development	914:939	development	929:939	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	0	49	theme	initial	48:54	arg1	formulas					77:84	initial and follow-on infant formulas	48:84	initial and follow-on infant formulas	48:84	[Expert consensus on the nutritional aspects of initial and follow-on infant formulas].
25801069	5	50	theme	professional	659:670	arg1	consensus					681:689	a professional criteria consensus	657:689	a professional criteria consensus on nutritional aspects of infant formulas	657:731	MATERIAL AND METHODS A survey, using 62 items, stratified into 5 blocks, was performed by 48 panelists using the Delphi method to achieve a professional criteria consensus on nutritional aspects of infant formulas.
25801069	4	51	theme	experts	469:475	arg1	panel					460:464	a panel	458:464	a panel of experts in infant gastroenterology	458:502	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	6	52	theme	non-unified	796:806	arg1	agreement					808:816	a non-unified agreement	794:816	a non-unified agreement	794:816	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	7	53	from	D	1101:1101	arg1	formulas					1129:1136	infant formulas	1122:1136	infant formulas	1122:1136	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	7	54	theme	correct	1145:1151	arg1	development					1182:1192	a correct cerebral, immune and somatic development	1143:1192	a correct cerebral, immune and somatic development	1143:1192	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	5	55	dep	survey	542:547	arg1	using					550:554	using	550:554	using 62 items	550:563	MATERIAL AND METHODS A survey, using 62 items, stratified into 5 blocks, was performed by 48 panelists using the Delphi method to achieve a professional criteria consensus on nutritional aspects of infant formulas.
25801069	5	56	from	consensus	681:689	arg1	aspects					706:712	nutritional aspects	694:712	nutritional aspects of infant formulas	694:731	MATERIAL AND METHODS A survey, using 62 items, stratified into 5 blocks, was performed by 48 panelists using the Delphi method to achieve a professional criteria consensus on nutritional aspects of infant formulas.
25801069	8	57	theme	well-defined	1233:1244	arg1	such					1256:1259	such	1256:1259	such	1256:1259	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	8	57	theme	well-defined	1233:1244	arg1	subjects					1246:1253	the not yet well-defined subjects	1221:1253	the not yet well-defined subjects	1221:1253	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	8	57	theme	well-defined	1233:1244	arg1	probiotic					1341:1349	probiotic	1341:1349	probiotic	1341:1349	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	8	57	theme	well-defined	1233:1244	arg1	aspects					1366:1372	symbiotic aspects	1356:1372	symbiotic aspects	1356:1372	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	7	58	from	calcium	1084:1090	arg1	formulas					1129:1136	infant formulas	1122:1136	infant formulas	1122:1136	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	2	59	theme	due	231:233	arg1	food					226:229	the perfect food	214:229	the perfect food due to its many benefits	214:254	Breastfeeding is the perfect food due to its many benefits.
25801069	2	59	theme	due	231:233	arg1	Breastfeeding					197:209	Breastfeeding	197:209	Breastfeeding	197:209	Breastfeeding is the perfect food due to its many benefits.
25801069	1	60	theme	first	123:127	arg1	months/years					129:140	the first months/years	119:140	the first months/years of life	119:148	INTRODUCTION Infant feeding in the first months/years of life affects the health in the short and long term.
25801069	5	61	dep	METHODS	532:538	arg1	survey					542:547	A survey	540:547	A survey	540:547	MATERIAL AND METHODS A survey, using 62 items, stratified into 5 blocks, was performed by 48 panelists using the Delphi method to achieve a professional criteria consensus on nutritional aspects of infant formulas.
25801069	6	62	theme	body	914:917	arg1	development					929:939	body and brain development	914:939	development	929:939	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	0	63	theme	follow-on	60:68	arg1	formulas					77:84	initial and follow-on infant formulas	48:84	initial and follow-on infant formulas	48:84	[Expert consensus on the nutritional aspects of initial and follow-on infant formulas].
25801069	8	64	theme	proteins	1287:1294	arg1	quality					1276:1282	nutritional quality	1264:1282	nutritional quality of proteins	1264:1294	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	8	64	theme	proteins	1287:1294	arg1	supplementation					1324:1338	taurine supplementation	1316:1338	taurine supplementation	1316:1338	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	8	64	theme	proteins	1287:1294	arg1	use					1297:1299	use	1297:1299	use of thickeners	1297:1313	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	6	65	theme	immune	945:950	arg1	maturity					952:959	immune maturity	945:959	immune maturity	945:959	RESULTS A consensus was reached in 64.6% of the items, with a non-unified agreement being established as regards the nutritional aspects of infant formulas, and their influence in body and brain development and immune maturity.
25801069	7	66	theme	infant	1122:1127	arg1	formulas					1129:1136	infant formulas	1122:1136	infant formulas	1122:1136	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	7	67	theme	suitable	1043:1050	arg1	composition					1052:1062	the suitable composition	1039:1062	the suitable composition of lipids	1039:1072	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	4	68	from	panel	460:464	arg1	gastroenterology					487:502	infant gastroenterology	480:502	infant gastroenterology	480:502	The aim of this study is to define the role of the supplemented formulas in infant nutrition using the opinion of a panel of experts in infant gastroenterology and nutrition.
25801069	2	69	theme	perfect	218:224	arg1	food					226:229	the perfect food	214:229	the perfect food due to its many benefits	214:254	Breastfeeding is the perfect food due to its many benefits.
25801069	2	69	theme	perfect	218:224	arg1	Breastfeeding					197:209	Breastfeeding	197:209	Breastfeeding	197:209	Breastfeeding is the perfect food due to its many benefits.
25801069	7	70	theme	lipids	1067:1072	arg1	calcium					1084:1090	calcium	1084:1090	calcium	1084:1090	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	7	70	theme	lipids	1067:1072	arg1	D					1101:1101	vitamin D	1093:1101	vitamin D	1093:1101	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	7	70	theme	lipids	1067:1072	arg1	prebiotics					1108:1117	prebiotics	1108:1117	prebiotics	1108:1117	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	7	70	theme	lipids	1067:1072	arg1	lactose					1075:1081	lactose	1075:1081	lactose	1075:1081	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	7	70	theme	lipids	1067:1072	arg1	composition					1052:1062	the suitable composition	1039:1062	the suitable composition of lipids	1039:1072	CONCLUSIONS According to the experts surveyed, there is consensus as regards the suitable composition of lipids, lactose, calcium, vitamin D, and prebiotics in infant formulas, for a correct cerebral, immune and somatic development.
25801069	8	71	theme	symbiotic	1356:1364	arg1	subjects					1246:1253	the not yet well-defined subjects	1221:1253	the not yet well-defined subjects	1221:1253	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25801069	8	71	theme	symbiotic	1356:1364	arg1	aspects					1366:1372	symbiotic aspects	1356:1372	symbiotic aspects	1356:1372	There was no consensus on the not yet well-defined subjects, such as nutritional quality of proteins, use of thickeners, taurine supplementation, probiotic, and symbiotic aspects.
25498708	9	0	theme	dehydrated	1196:1205	arg1	products					1214:1221	dehydrated potato products	1196:1221	dehydrated potato products	1196:1221	Overall, potato parenchyma cells may expand the industrial availability of dehydrated potato products.
25498708	0	1	theme	gelatinized	88:98	arg1	starches					100:107	ungelatinized and gelatinized starches	70:107	ungelatinized and gelatinized starches	70:107	Physicochemical properties of dehydrated potato parenchyma cells with ungelatinized and gelatinized starches.
25498708	5	2	theme	intact	778:783	arg1	walls					801:805	intact parenchyma cell walls	778:805	intact parenchyma cell walls	778:805	Ungelatinized and gelatinized starches within parenchyma cells were tightly wrapped by intact parenchyma cell walls.
25498708	9	3	theme	potato	1207:1212	arg1	products					1214:1221	dehydrated potato products	1196:1221	dehydrated potato products	1196:1221	Overall, potato parenchyma cells may expand the industrial availability of dehydrated potato products.
25498708	1	4	theme	intact	181:186	arg1	walls					204:208	intact parenchyma cell walls	181:208	intact parenchyma cell walls	181:208	Potato parenchyma cells were characterized to identify the function of intact parenchyma cell walls on their physicochemical properties.
25498708	9	5	theme	products	1214:1221	arg1	availability					1180:1191	the industrial availability	1165:1191	the industrial availability of dehydrated potato products	1165:1221	Overall, potato parenchyma cells may expand the industrial availability of dehydrated potato products.
25498708	9	6	theme	industrial	1169:1178	arg1	availability					1180:1191	the industrial availability	1165:1191	the industrial availability of dehydrated potato products	1165:1221	Overall, potato parenchyma cells may expand the industrial availability of dehydrated potato products.
25498708	5	7	theme	Ungelatinized	691:703	arg1	starches					721:728	Ungelatinized and gelatinized starches	691:728	Ungelatinized and gelatinized starches within parenchyma cells	691:752	Ungelatinized and gelatinized starches within parenchyma cells were tightly wrapped by intact parenchyma cell walls.
25498708	8	8	theme	native	1099:1104	arg1	starch					1113:1118	native potato starch	1099:1118	native potato starch	1099:1118	R-Cell revealed the increased gelatinization temperatures than native potato starch.
25498708	9	9	theme	potato	1130:1135	arg1	cells					1148:1152	potato parenchyma cells	1130:1152	potato parenchyma cells	1130:1152	Overall, potato parenchyma cells may expand the industrial availability of dehydrated potato products.
25498708	4	10	theme	cell	675:678	arg1	isolation					680:688	parenchyma cell isolation	664:688	parenchyma cell isolation	664:688	Protein and ash were lost during parenchyma cell isolation.
25498708	3	11	theme	Potato	506:511	arg1	controls					586:593	controls	586:593	controls of R-Cell and G-Cell	586:614	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	11	theme	Potato	506:511	arg1	PGL					545:547	potato granule (PGL)	529:548	potato granule (PGL)	529:548	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	11	theme	Potato	506:511	arg1	flour					513:517	Potato flour	506:517	Potato flour (RPF)	506:523	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	11	theme	Potato	506:511	arg1	RPF					520:522	RPF	520:522	RPF	520:522	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	8	12	theme	gelatinization	1066:1079	arg1	temperatures					1081:1092	the increased gelatinization temperatures	1052:1092	the increased gelatinization temperatures than native potato starch	1052:1118	R-Cell revealed the increased gelatinization temperatures than native potato starch.
25498708	2	13	theme	starch	432:437	arg1	leaching					439:446	starch leaching	432:446	starch leaching	432:446	Parenchyma cells were separated using pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively), and investigated with respect to their morphology, chemical composition, starch leaching and swelling power, gelatinization, and pasting property.
25498708	2	14	theme	cooked	308:313	arg1	potatoes					315:322	cooked potatoes	308:322	cooked potatoes (R-Cell and G-Cell, respectively)	308:356	Parenchyma cells were separated using pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively), and investigated with respect to their morphology, chemical composition, starch leaching and swelling power, gelatinization, and pasting property.
25498708	1	15	theme	parenchyma	188:197	arg1	walls					204:208	intact parenchyma cell walls	181:208	intact parenchyma cell walls	181:208	Potato parenchyma cells were characterized to identify the function of intact parenchyma cell walls on their physicochemical properties.
25498708	2	16	theme	pasting	488:494	arg1	property					496:503	pasting property	488:503	pasting property	488:503	Parenchyma cells were separated using pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively), and investigated with respect to their morphology, chemical composition, starch leaching and swelling power, gelatinization, and pasting property.
25498708	7	17	theme	swelling	941:948	arg1	powers					950:955	swelling powers	941:955	swelling powers	941:955	R-Cell exhibited the reduced swelling powers and pasting viscosities, while the opposite patterns were observed for G-Cell.
25498708	3	18	theme	granule	536:542	arg1	controls					586:593	controls	586:593	controls of R-Cell and G-Cell	586:614	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	18	theme	granule	536:542	arg1	PGL					545:547	potato granule (PGL)	529:548	potato granule (PGL)	529:548	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	18	theme	granule	536:542	arg1	flour					513:517	Potato flour	506:517	Potato flour (RPF)	506:523	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	1	19	theme	cell	199:202	arg1	walls					204:208	intact parenchyma cell walls	181:208	intact parenchyma cell walls	181:208	Potato parenchyma cells were characterized to identify the function of intact parenchyma cell walls on their physicochemical properties.
25498708	1	20	theme	walls	204:208	arg1	function					169:176	the function	165:176	the function of intact parenchyma cell walls on their physicochemical properties	165:244	Potato parenchyma cells were characterized to identify the function of intact parenchyma cell walls on their physicochemical properties.
25498708	0	21	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of dehydrated potato parenchyma cells with ungelatinized and gelatinized starches	0:107	Physicochemical properties of dehydrated potato parenchyma cells with ungelatinized and gelatinized starches.
25498708	1	22	theme	Potato	110:115	arg1	cells					128:132	Potato parenchyma cells	110:132	Potato parenchyma cells	110:132	Potato parenchyma cells were characterized to identify the function of intact parenchyma cell walls on their physicochemical properties.
25498708	0	23	theme	dehydrated	30:39	arg1	cells					59:63	dehydrated potato parenchyma cells	30:63	dehydrated potato parenchyma cells with ungelatinized and gelatinized starches	30:107	Physicochemical properties of dehydrated potato parenchyma cells with ungelatinized and gelatinized starches.
25498708	8	24	theme	potato	1106:1111	arg1	starch					1113:1118	native potato starch	1099:1118	native potato starch	1099:1118	R-Cell revealed the increased gelatinization temperatures than native potato starch.
25498708	1	25	from	function	169:176	arg1	properties					235:244	their physicochemical properties	213:244	their physicochemical properties	213:244	Potato parenchyma cells were characterized to identify the function of intact parenchyma cell walls on their physicochemical properties.
25498708	1	26	theme	parenchyma	117:126	arg1	cells					128:132	Potato parenchyma cells	110:132	Potato parenchyma cells	110:132	Potato parenchyma cells were characterized to identify the function of intact parenchyma cell walls on their physicochemical properties.
25498708	2	27	theme	leaching	439:446	arg1	power					461:465	starch leaching and swelling power	432:465	starch leaching and swelling power	432:465	Parenchyma cells were separated using pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively), and investigated with respect to their morphology, chemical composition, starch leaching and swelling power, gelatinization, and pasting property.
25498708	3	28	theme	potato	529:534	arg1	controls					586:593	controls	586:593	controls of R-Cell and G-Cell	586:614	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	28	theme	potato	529:534	arg1	PGL					545:547	potato granule (PGL)	529:548	potato granule (PGL)	529:548	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	28	theme	potato	529:534	arg1	flour					513:517	Potato flour	506:517	Potato flour (RPF)	506:523	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	6	29	theme	starch	872:877	arg1	leaching					879:886	starch leaching	872:886	starch leaching	872:886	Compared to their controls, the parenchyma cell walls prevented starch leaching from R-Cell and G-Cell.
25498708	2	30	dep	potatoes	315:322	arg1	G-Cell					336:341	G-Cell	336:341	G-Cell	336:341	Parenchyma cells were separated using pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively), and investigated with respect to their morphology, chemical composition, starch leaching and swelling power, gelatinization, and pasting property.
25498708	2	30	dep	potatoes	315:322	arg1	R-Cell					325:330	R-Cell	325:330	R-Cell	325:330	Parenchyma cells were separated using pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively), and investigated with respect to their morphology, chemical composition, starch leaching and swelling power, gelatinization, and pasting property.
25498708	2	31	from	raw	300:302	arg1	pectinase					285:293	pectinase	285:293	pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively)	285:356	Parenchyma cells were separated using pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively), and investigated with respect to their morphology, chemical composition, starch leaching and swelling power, gelatinization, and pasting property.
25498708	1	32	theme	physicochemical	219:233	arg1	properties					235:244	their physicochemical properties	213:244	their physicochemical properties	213:244	Potato parenchyma cells were characterized to identify the function of intact parenchyma cell walls on their physicochemical properties.
25498708	0	33	theme	parenchyma	48:57	arg1	cells					59:63	dehydrated potato parenchyma cells	30:63	dehydrated potato parenchyma cells with ungelatinized and gelatinized starches	30:107	Physicochemical properties of dehydrated potato parenchyma cells with ungelatinized and gelatinized starches.
25498708	7	34	theme	pasting	961:967	arg1	viscosities					969:979	pasting viscosities	961:979	pasting viscosities	961:979	R-Cell exhibited the reduced swelling powers and pasting viscosities, while the opposite patterns were observed for G-Cell.
25498708	5	35	theme	gelatinized	709:719	arg1	starches					721:728	Ungelatinized and gelatinized starches	691:728	Ungelatinized and gelatinized starches within parenchyma cells	691:752	Ungelatinized and gelatinized starches within parenchyma cells were tightly wrapped by intact parenchyma cell walls.
25498708	9	36	theme	parenchyma	1137:1146	arg1	cells					1148:1152	potato parenchyma cells	1130:1152	potato parenchyma cells	1130:1152	Overall, potato parenchyma cells may expand the industrial availability of dehydrated potato products.
25498708	0	37	theme	potato	41:46	arg1	cells					59:63	dehydrated potato parenchyma cells	30:63	dehydrated potato parenchyma cells with ungelatinized and gelatinized starches	30:107	Physicochemical properties of dehydrated potato parenchyma cells with ungelatinized and gelatinized starches.
25498708	5	38	theme	parenchyma	785:794	arg1	walls					801:805	intact parenchyma cell walls	778:805	intact parenchyma cell walls	778:805	Ungelatinized and gelatinized starches within parenchyma cells were tightly wrapped by intact parenchyma cell walls.
25498708	2	39	theme	swelling	452:459	arg1	power					461:465	starch leaching and swelling power	432:465	starch leaching and swelling power	432:465	Parenchyma cells were separated using pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively), and investigated with respect to their morphology, chemical composition, starch leaching and swelling power, gelatinization, and pasting property.
25498708	5	40	theme	cell	796:799	arg1	walls					801:805	intact parenchyma cell walls	778:805	intact parenchyma cell walls	778:805	Ungelatinized and gelatinized starches within parenchyma cells were tightly wrapped by intact parenchyma cell walls.
25498708	6	41	theme	cell	851:854	arg1	walls					856:860	the parenchyma cell walls	836:860	the parenchyma cell walls	836:860	Compared to their controls, the parenchyma cell walls prevented starch leaching from R-Cell and G-Cell.
25498708	3	42	used	used	578:581	arg2	PGL					545:547	potato granule (PGL)	529:548	potato granule (PGL)	529:548	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	42	used	used	578:581	arg2	RPF					520:522	RPF	520:522	RPF	520:522	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	42	used	used	578:581	arg2	flour					513:517	Potato flour	506:517	Potato flour (RPF)	506:523	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	42	used	used	578:581	arg2	controls					586:593	controls	586:593	controls of R-Cell and G-Cell	586:614	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	0	43	theme	cells	59:63	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of dehydrated potato parenchyma cells with ungelatinized and gelatinized starches	0:107	Physicochemical properties of dehydrated potato parenchyma cells with ungelatinized and gelatinized starches.
25498708	2	44	theme	chemical	410:417	arg1	composition					419:429	chemical composition	410:429	chemical composition	410:429	Parenchyma cells were separated using pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively), and investigated with respect to their morphology, chemical composition, starch leaching and swelling power, gelatinization, and pasting property.
25498708	6	45	theme	parenchyma	840:849	arg1	walls					856:860	the parenchyma cell walls	836:860	the parenchyma cell walls	836:860	Compared to their controls, the parenchyma cell walls prevented starch leaching from R-Cell and G-Cell.
25498708	2	46	theme	Parenchyma	247:256	arg1	cells					258:262	Parenchyma cells	247:262	Parenchyma cells	247:262	Parenchyma cells were separated using pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively), and investigated with respect to their morphology, chemical composition, starch leaching and swelling power, gelatinization, and pasting property.
25498708	8	47	theme	increased	1056:1064	arg1	temperatures					1081:1092	the increased gelatinization temperatures	1052:1092	the increased gelatinization temperatures than native potato starch	1052:1118	R-Cell revealed the increased gelatinization temperatures than native potato starch.
25498708	7	48	theme	reduced	933:939	arg1	powers					950:955	swelling powers	941:955	swelling powers	941:955	R-Cell exhibited the reduced swelling powers and pasting viscosities, while the opposite patterns were observed for G-Cell.
25498708	5	49	theme	parenchyma	737:746	arg1	cells					748:752	parenchyma cells	737:752	parenchyma cells	737:752	Ungelatinized and gelatinized starches within parenchyma cells were tightly wrapped by intact parenchyma cell walls.
25498708	3	50	theme	G-Cell	609:614	arg1	PGL					545:547	potato granule (PGL)	529:548	potato granule (PGL)	529:548	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	50	theme	G-Cell	609:614	arg1	controls					586:593	controls	586:593	controls of R-Cell and G-Cell	586:614	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	50	theme	G-Cell	609:614	arg1	flour					513:517	Potato flour	506:517	Potato flour (RPF)	506:523	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	7	51	theme	opposite	992:999	arg1	patterns					1001:1008	the opposite patterns	988:1008	the opposite patterns	988:1008	R-Cell exhibited the reduced swelling powers and pasting viscosities, while the opposite patterns were observed for G-Cell.
25498708	0	52	with	cells	59:63	arg1	starches					100:107	ungelatinized and gelatinized starches	70:107	ungelatinized and gelatinized starches	70:107	Physicochemical properties of dehydrated potato parenchyma cells with ungelatinized and gelatinized starches.
25498708	4	53	theme	parenchyma	664:673	arg1	isolation					680:688	parenchyma cell isolation	664:688	parenchyma cell isolation	664:688	Protein and ash were lost during parenchyma cell isolation.
25498708	2	54	from	potatoes	315:322	arg1	pectinase					285:293	pectinase	285:293	pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively)	285:356	Parenchyma cells were separated using pectinase from raw and cooked potatoes (R-Cell and G-Cell, respectively), and investigated with respect to their morphology, chemical composition, starch leaching and swelling power, gelatinization, and pasting property.
25498708	0	55	theme	ungelatinized	70:82	arg1	starches					100:107	ungelatinized and gelatinized starches	70:107	ungelatinized and gelatinized starches	70:107	Physicochemical properties of dehydrated potato parenchyma cells with ungelatinized and gelatinized starches.
25498708	3	56	theme	R-Cell	598:603	arg1	PGL					545:547	potato granule (PGL)	529:548	potato granule (PGL)	529:548	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	56	theme	R-Cell	598:603	arg1	controls					586:593	controls	586:593	controls of R-Cell and G-Cell	586:614	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
25498708	3	56	theme	R-Cell	598:603	arg1	flour					513:517	Potato flour	506:517	Potato flour (RPF)	506:523	Potato flour (RPF) and potato granule (PGL) prepared in laboratory were used as controls of R-Cell and G-Cell, respectively.
27612644	7	0	theme	higher	1355:1360	arg1	potential					1362:1370	the higher potential	1351:1370	the higher potential of chitosan/Al2O3/Fe3O4 composite nanofibers for nitrate and phosphate	1351:1441	The obtained results revealed the higher potential of chitosan/Al2O3/Fe3O4 composite nanofibers for nitrate and phosphate compared with chitosan/Al2O3/Fe3O4 composite beads.
27612644	2	1	dep	nitrate	516:522	arg1	the					512:514	the	512:514	the	512:514	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	2	2	theme	content	421:427	arg1	influence					386:394	The influence	382:394	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents	382:595	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	4	3	theme	phosphate	917:925	arg1	removal					927:933	phosphate removal	917:933	phosphate removal	917:933	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	0	4	with	Comparison	93:102	arg1	beads					130:134	chitosan/Al2O3/Fe3O4 beads	109:134	chitosan/Al2O3/Fe3O4 beads	109:134	Removal of nitrate and phosphate using chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent: Comparison with chitosan/Al2O3/Fe3O4 beads.
27612644	2	5	theme	composite	411:419	arg1	content					421:427	Al2O3/Fe3O4 composite content	399:427	Al2O3/Fe3O4 composite content	399:427	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	1	6	theme	nitrate	294:300	arg1	removal					283:289	the removal	279:289	the removal of nitrate and phosphate	279:314	In the present study the chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent was prepared by electrospinning process and its application for the removal of nitrate and phosphate were compared with chitosan/Al2O3/Fe3O4 composite bead adsorbent.
27612644	0	7	theme	chitosan/Al2O3/Fe3O4	109:128	arg1	beads					130:134	chitosan/Al2O3/Fe3O4 beads	109:134	chitosan/Al2O3/Fe3O4 beads	109:134	Removal of nitrate and phosphate using chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent: Comparison with chitosan/Al2O3/Fe3O4 beads.
27612644	5	8	theme	sorption	1115:1122	arg1	kinetic					1061:1067	kinetic	1061:1067	kinetic	1061:1067	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	5	9	theme	composite	1151:1159	arg1	beads					1161:1165	chitosan/Al2O3/Fe3O4 composite beads	1130:1165	chitosan/Al2O3/Fe3O4 composite beads	1130:1165	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	5	10	theme	equilibrium	1073:1083	arg1	data					1085:1088	equilibrium data	1073:1088	equilibrium data	1073:1088	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	6	11	theme	phosphate	1297:1305	arg1	efficiency					1271:1280	the sorption efficiency	1258:1280	the sorption efficiency of nitrate and phosphate	1258:1305	The influence of other anions including chloride, fluoride and sulphate on the sorption efficiency of nitrate and phosphate was examined.
27612644	7	12	theme	nanofibers	1406:1415	arg1	potential					1362:1370	the higher potential	1351:1370	the higher potential of chitosan/Al2O3/Fe3O4 composite nanofibers for nitrate and phosphate	1351:1441	The obtained results revealed the higher potential of chitosan/Al2O3/Fe3O4 composite nanofibers for nitrate and phosphate compared with chitosan/Al2O3/Fe3O4 composite beads.
27612644	4	13	theme	nitrate	853:859	arg1	effects					824:830	the interaction effects	808:830	the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency	808:944	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	1	14	theme	phosphate	306:314	arg1	removal					283:289	the removal	279:289	the removal of nitrate and phosphate	279:314	In the present study the chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent was prepared by electrospinning process and its application for the removal of nitrate and phosphate were compared with chitosan/Al2O3/Fe3O4 composite bead adsorbent.
27612644	2	15	theme	nanofibrous	574:584	arg1	adsorbents					586:595	synthesized bead and nanofibrous adsorbents	553:595	synthesized bead and nanofibrous adsorbents	553:595	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	4	16	theme	initial	875:881	arg1	concentrations					883:896	phosphate initial concentrations	865:896	phosphate initial concentrations	865:896	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	4	17	theme	Box-Behnken	765:775	arg1	design					777:782	The Box-Behnken design	761:782	The Box-Behnken design	761:782	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	1	18	theme	adsorbent	205:213	arg1	chitosan/Al2O3/Fe3O4					162:181	the chitosan/Al2O3/Fe3O4	158:181	the chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent	158:213	In the present study the chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent was prepared by electrospinning process and its application for the removal of nitrate and phosphate were compared with chitosan/Al2O3/Fe3O4 composite bead adsorbent.
27612644	4	19	used	used	788:791	arg2	design					777:782	The Box-Behnken design	761:782	The Box-Behnken design	761:782	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	4	20	theme	phosphate	865:873	arg1	concentrations					883:896	phosphate initial concentrations	865:896	phosphate initial concentrations	865:896	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	2	21	theme	time	442:445	arg1	influence					386:394	The influence	382:394	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents	382:595	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	2	22	theme	bead	565:568	arg1	adsorbents					586:595	synthesized bead and nanofibrous adsorbents	553:595	synthesized bead and nanofibrous adsorbents	553:595	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	4	23	theme	adsorbent	835:843	arg1	dosage					845:850	adsorbent dosage	835:850	adsorbent dosage	835:850	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	6	24	theme	anions	1206:1211	arg1	influence					1187:1195	The influence	1183:1195	The influence of other anions including chloride, fluoride and sulphate on the sorption efficiency of nitrate and phosphate	1183:1305	The influence of other anions including chloride, fluoride and sulphate on the sorption efficiency of nitrate and phosphate was examined.
27612644	2	25	theme	contact	434:440	arg1	time					442:445	contact time	434:445	contact time	434:445	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	2	26	theme	temperature	497:507	arg1	influence					386:394	The influence	382:394	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents	382:595	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	5	27	theme	Freundlich	995:1004	arg1	models					1028:1033	known Freundlich and Langmuir isotherm models	989:1033	known Freundlich and Langmuir isotherm models	989:1033	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	5	28	used	used	1040:1043	arg2	models					1028:1033	known Freundlich and Langmuir isotherm models	989:1033	known Freundlich and Langmuir isotherm models	989:1033	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	5	28	used	used	1040:1043	arg2	model					979:983	The pseudo-second-order kinetic model	947:983	The pseudo-second-order kinetic model	947:983	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	2	29	theme	single	619:624	arg1	system					626:631	a single system	617:631	a single system	617:631	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	4	30	theme	removal	927:933	arg1	efficiency					935:944	the nitrate and phosphate removal efficiency	901:944	the nitrate and phosphate removal efficiency	901:944	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	5	31	dep	kinetic	1061:1067	arg1	the					1057:1059	the	1057:1059	the	1057:1059	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	2	32	from	influence	386:394	arg1	nitrate					516:522	nitrate	516:522	nitrate	516:522	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	2	32	from	influence	386:394	arg1	sorption					538:545	phosphate sorption	528:545	phosphate sorption	528:545	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	1	33	theme	electrospinning	231:245	arg1	process					247:253	electrospinning process	231:253	electrospinning process	231:253	In the present study the chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent was prepared by electrospinning process and its application for the removal of nitrate and phosphate were compared with chitosan/Al2O3/Fe3O4 composite bead adsorbent.
27612644	0	34	theme	nitrate	11:17	arg1	Removal					0:6	Removal	0:6	Removal of nitrate and phosphate	0:31	Removal of nitrate and phosphate using chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent: Comparison with chitosan/Al2O3/Fe3O4 beads.
27612644	5	35	theme	nitrate	1093:1099	arg1	kinetic					1061:1067	kinetic	1061:1067	kinetic	1061:1067	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	4	36	theme	dosage	845:850	arg1	effects					824:830	the interaction effects	808:830	the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency	808:944	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	2	37	theme	phosphate	528:536	arg1	sorption					538:545	phosphate sorption	528:545	phosphate sorption	528:545	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	7	38	theme	composite	1478:1486	arg1	beads					1488:1492	chitosan/Al2O3/Fe3O4 composite beads	1457:1492	chitosan/Al2O3/Fe3O4 composite beads	1457:1492	The obtained results revealed the higher potential of chitosan/Al2O3/Fe3O4 composite nanofibers for nitrate and phosphate compared with chitosan/Al2O3/Fe3O4 composite beads.
27612644	7	39	theme	chitosan/Al2O3/Fe3O4	1457:1476	arg1	beads					1488:1492	chitosan/Al2O3/Fe3O4 composite beads	1457:1492	chitosan/Al2O3/Fe3O4 composite beads	1457:1492	The obtained results revealed the higher potential of chitosan/Al2O3/Fe3O4 composite nanofibers for nitrate and phosphate compared with chitosan/Al2O3/Fe3O4 composite beads.
27612644	3	40	theme	composite	674:682	arg1	beads					684:688	chitosan/Al2O3/Fe3O4 composite beads	653:688	chitosan/Al2O3/Fe3O4 composite beads	653:688	The reusability of chitosan/Al2O3/Fe3O4 composite beads and nanofibers after five sorption-desorption cycles were carried out.
27612644	5	41	theme	isotherm	1019:1026	arg1	models					1028:1033	known Freundlich and Langmuir isotherm models	989:1033	known Freundlich and Langmuir isotherm models	989:1033	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	5	42	theme	pseudo-second-order	951:969	arg1	model					979:983	The pseudo-second-order kinetic model	947:983	The pseudo-second-order kinetic model	947:983	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	0	43	theme	phosphate	23:31	arg1	Removal					0:6	Removal	0:6	Removal of nitrate and phosphate	0:31	Removal of nitrate and phosphate using chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent: Comparison with chitosan/Al2O3/Fe3O4 beads.
27612644	5	44	theme	known	989:993	arg1	models					1028:1033	known Freundlich and Langmuir isotherm models	989:1033	known Freundlich and Langmuir isotherm models	989:1033	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	3	45	theme	sorption-desorption	716:734	arg1	cycles					736:741	five sorption-desorption cycles	711:741	five sorption-desorption cycles	711:741	The reusability of chitosan/Al2O3/Fe3O4 composite beads and nanofibers after five sorption-desorption cycles were carried out.
27612644	2	46	dep	nitrate	448:454	arg1	concentrations					478:491	initial concentrations	470:491	initial concentrations	470:491	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	2	47	theme	nitrate	448:454	arg1	influence					386:394	The influence	382:394	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents	382:595	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	0	48	theme	composite	60:68	arg1	nanofibrous					70:80	chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent	39:90	chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent	39:90	Removal of nitrate and phosphate using chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent: Comparison with chitosan/Al2O3/Fe3O4 beads.
27612644	7	49	theme	chitosan/Al2O3/Fe3O4	1375:1394	arg1	nanofibers					1406:1415	chitosan/Al2O3/Fe3O4 composite nanofibers	1375:1415	chitosan/Al2O3/Fe3O4 composite nanofibers	1375:1415	The obtained results revealed the higher potential of chitosan/Al2O3/Fe3O4 composite nanofibers for nitrate and phosphate compared with chitosan/Al2O3/Fe3O4 composite beads.
27612644	0	50	dep	using	33:37	arg1	Comparison					93:102	Comparison	93:102	Comparison with chitosan/Al2O3/Fe3O4 beads	93:134	Removal of nitrate and phosphate using chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent: Comparison with chitosan/Al2O3/Fe3O4 beads.
27612644	2	51	theme	Al2O3/Fe3O4	399:409	arg1	content					421:427	Al2O3/Fe3O4 composite content	399:427	Al2O3/Fe3O4 composite content	399:427	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	0	52	theme	chitosan/Al2O3/Fe3O4	39:58	arg1	nanofibrous					70:80	chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent	39:90	chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent	39:90	Removal of nitrate and phosphate using chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent: Comparison with chitosan/Al2O3/Fe3O4 beads.
27612644	1	53	theme	adsorbent	371:379	arg1	chitosan/Al2O3/Fe3O4					335:354	chitosan/Al2O3/Fe3O4	335:354	chitosan/Al2O3/Fe3O4 composite bead adsorbent	335:379	In the present study the chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent was prepared by electrospinning process and its application for the removal of nitrate and phosphate were compared with chitosan/Al2O3/Fe3O4 composite bead adsorbent.
27612644	6	54	theme	other	1200:1204	arg1	anions					1206:1211	other anions	1200:1211	other anions including chloride, fluoride and sulphate	1200:1253	The influence of other anions including chloride, fluoride and sulphate on the sorption efficiency of nitrate and phosphate was examined.
27612644	6	54	theme	other	1200:1204	arg1	sulphate					1246:1253	sulphate	1246:1253	sulphate	1246:1253	The influence of other anions including chloride, fluoride and sulphate on the sorption efficiency of nitrate and phosphate was examined.
27612644	6	54	theme	other	1200:1204	arg1	fluoride					1233:1240	fluoride	1233:1240	fluoride	1233:1240	The influence of other anions including chloride, fluoride and sulphate on the sorption efficiency of nitrate and phosphate was examined.
27612644	6	54	theme	other	1200:1204	arg1	chloride					1223:1230	chloride	1223:1230	chloride	1223:1230	The influence of other anions including chloride, fluoride and sulphate on the sorption efficiency of nitrate and phosphate was examined.
27612644	3	55	theme	chitosan/Al2O3/Fe3O4	653:672	arg1	beads					684:688	chitosan/Al2O3/Fe3O4 composite beads	653:688	chitosan/Al2O3/Fe3O4 composite beads	653:688	The reusability of chitosan/Al2O3/Fe3O4 composite beads and nanofibers after five sorption-desorption cycles were carried out.
27612644	6	56	theme	sorption	1262:1269	arg1	efficiency					1271:1280	the sorption efficiency	1258:1280	the sorption efficiency of nitrate and phosphate	1258:1305	The influence of other anions including chloride, fluoride and sulphate on the sorption efficiency of nitrate and phosphate was examined.
27612644	4	57	from	effects	824:830	arg1	efficiency					935:944	the nitrate and phosphate removal efficiency	901:944	the nitrate and phosphate removal efficiency	901:944	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	6	58	from	influence	1187:1195	arg1	efficiency					1271:1280	the sorption efficiency	1258:1280	the sorption efficiency of nitrate and phosphate	1258:1305	The influence of other anions including chloride, fluoride and sulphate on the sorption efficiency of nitrate and phosphate was examined.
27612644	5	59	theme	phosphate	1105:1113	arg1	sorption					1115:1122	phosphate sorption	1105:1122	phosphate sorption	1105:1122	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	3	60	theme	beads	684:688	arg1	reusability					638:648	The reusability	634:648	The reusability of chitosan/Al2O3/Fe3O4 composite beads and nanofibers after five sorption-desorption cycles	634:741	The reusability of chitosan/Al2O3/Fe3O4 composite beads and nanofibers after five sorption-desorption cycles were carried out.
27612644	1	61	theme	present	144:150	arg1	study					152:156	the present study	140:156	the present study	140:156	In the present study the chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent was prepared by electrospinning process and its application for the removal of nitrate and phosphate were compared with chitosan/Al2O3/Fe3O4 composite bead adsorbent.
27612644	6	62	theme	nitrate	1285:1291	arg1	efficiency					1271:1280	the sorption efficiency	1258:1280	the sorption efficiency of nitrate and phosphate	1258:1305	The influence of other anions including chloride, fluoride and sulphate on the sorption efficiency of nitrate and phosphate was examined.
27612644	4	63	theme	concentrations	883:896	arg1	effects					824:830	the interaction effects	808:830	the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency	808:944	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	4	64	theme	interaction	812:822	arg1	effects					824:830	the interaction effects	808:830	the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency	808:944	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	0	65	theme	adsorbent	82:90	arg1	nanofibrous					70:80	chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent	39:90	chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent	39:90	Removal of nitrate and phosphate using chitosan/Al2O3/Fe3O4 composite nanofibrous adsorbent: Comparison with chitosan/Al2O3/Fe3O4 beads.
27612644	5	66	theme	kinetic	971:977	arg1	model					979:983	The pseudo-second-order kinetic model	947:983	The pseudo-second-order kinetic model	947:983	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	4	67	theme	nitrate	905:911	arg1	efficiency					935:944	the nitrate and phosphate removal efficiency	901:944	the nitrate and phosphate removal efficiency	901:944	The Box-Behnken design was used to investigate the interaction effects of adsorbent dosage, nitrate and phosphate initial concentrations on the nitrate and phosphate removal efficiency.
27612644	2	68	theme	initial	470:476	arg1	concentrations					478:491	initial concentrations	470:491	initial concentrations	470:491	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	2	69	theme	pH	430:431	arg1	influence					386:394	The influence	382:394	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents	382:595	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	2	70	theme	synthesized	553:563	arg1	adsorbents					586:595	synthesized bead and nanofibrous adsorbents	553:595	synthesized bead and nanofibrous adsorbents	553:595	The influence of Al2O3/Fe3O4 composite content, pH, contact time, nitrate and phosphate initial concentrations and temperature on the nitrate and phosphate sorption using synthesized bead and nanofibrous adsorbents was investigated in a single system.
27612644	7	71	theme	obtained	1325:1332	arg1	results					1334:1340	The obtained results	1321:1340	The obtained results	1321:1340	The obtained results revealed the higher potential of chitosan/Al2O3/Fe3O4 composite nanofibers for nitrate and phosphate compared with chitosan/Al2O3/Fe3O4 composite beads.
27612644	3	72	theme	nanofibers	694:703	arg1	reusability					638:648	The reusability	634:648	The reusability of chitosan/Al2O3/Fe3O4 composite beads and nanofibers after five sorption-desorption cycles	634:741	The reusability of chitosan/Al2O3/Fe3O4 composite beads and nanofibers after five sorption-desorption cycles were carried out.
27612644	5	73	theme	Langmuir	1010:1017	arg1	isotherm					1019:1026	Langmuir isotherm	1010:1026	Langmuir isotherm	1010:1026	The pseudo-second-order kinetic model and known Freundlich and Langmuir isotherm models were used to describe the kinetic and equilibrium data of nitrate and phosphate sorption using chitosan/Al2O3/Fe3O4 composite beads and nanofibers.
27612644	7	74	theme	composite	1396:1404	arg1	nanofibers					1406:1415	chitosan/Al2O3/Fe3O4 composite nanofibers	1375:1415	chitosan/Al2O3/Fe3O4 composite nanofibers	1375:1415	The obtained results revealed the higher potential of chitosan/Al2O3/Fe3O4 composite nanofibers for nitrate and phosphate compared with chitosan/Al2O3/Fe3O4 composite beads.
28552410	0	0	theme	fungal	84:89	arg1	composition					101:111	fungal cell wall composition	84:111	fungal cell wall composition	84:111	Lung eosinophil recruitment in response to Aspergillus fumigatus is correlated with fungal cell wall composition and requires γδ T cells.
28552410	1	1	theme	interest	343:350	arg1	focus					321:325	a focus	319:325	a focus of considerable interest	319:350	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	1	1	theme	interest	343:350	arg1	recognition					155:165	The differential recognition	138:165	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus	138:308	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	2	2	from	levels	419:424	arg1	isolates					497:504	A. fumigatus isolates	484:504	A. fumigatus isolates	484:504	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	2	2	from	levels	419:424	arg1	strains					517:523	mutant strains	510:523	mutant strains	510:523	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	1	3	theme	differential	142:153	arg1	focus					321:325	a focus	319:325	a focus of considerable interest	319:350	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	1	3	theme	differential	142:153	arg1	recognition					155:165	The differential recognition	138:165	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus	138:308	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	2	4	theme	A.	484:485	arg1	isolates					497:504	A. fumigatus isolates	484:504	A. fumigatus isolates	484:504	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	5	5	theme	innate-like	1089:1099	arg1	lymphocytes					1101:1111	innate-like lymphocytes	1089:1111	innate-like lymphocytes	1089:1111	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	1	6	theme	human	252:256	arg1	pathogen					279:286	the human opportunistic fungal pathogen	248:286	the human opportunistic fungal pathogen Aspergillus fumigatus	248:308	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	0	7	theme	wall	96:99	arg1	composition					101:111	fungal cell wall composition	84:111	fungal cell wall composition	84:111	Lung eosinophil recruitment in response to Aspergillus fumigatus is correlated with fungal cell wall composition and requires γδ T cells.
28552410	1	8	dep	Aspergillus	288:298	arg1	fumigatus					300:308	fumigatus	300:308	fumigatus	300:308	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	5	9	theme	detrimental	1158:1168	arg1	responses					1184:1192	potentially detrimental type 2 immune responses	1146:1192	potentially detrimental type 2 immune responses	1146:1192	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	1	10	theme	opportunistic	258:270	arg1	pathogen					279:286	the human opportunistic fungal pathogen	248:286	the human opportunistic fungal pathogen Aspergillus fumigatus	248:308	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	0	11	theme	cell	91:94	arg1	composition					101:111	fungal cell wall composition	84:111	fungal cell wall composition	84:111	Lung eosinophil recruitment in response to Aspergillus fumigatus is correlated with fungal cell wall composition and requires γδ T cells.
28552410	5	12	theme	important	1123:1131	arg1	regulator					1133:1141	an important regulator	1120:1141	an important regulator of potentially detrimental type 2 immune responses	1120:1192	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	5	12	theme	important	1123:1131	arg1	subset					1079:1084	this subset	1074:1084	this subset of innate-like lymphocytes	1074:1111	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	4	13	theme	single	923:928	arg1	aspiration					930:939	a single aspiration	921:939	a single aspiration	921:939	The response to increased A. fumigatus chitin was associated with increased transcription of IL-17A after a single aspiration, although this cytokine was not required for eosinophil recruitment.
28552410	2	14	theme	mouse	358:362	arg1	model					364:368	a mouse model	356:368	a mouse model of fungal conidia aspiration	356:397	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	1	15	theme	fungal	272:277	arg1	pathogen					279:286	the human opportunistic fungal pathogen	248:286	the human opportunistic fungal pathogen Aspergillus fumigatus	248:308	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	5	16	theme	RAG1	1023:1026	arg1	cells					1037:1041	both RAG1 and γδ T cells	1018:1041	both RAG1 and γδ T cells	1018:1041	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	2	17	theme	conidia	380:386	arg1	aspiration					388:397	fungal conidia aspiration	373:397	fungal conidia aspiration	373:397	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	2	18	theme	mutant	510:515	arg1	strains					517:523	mutant strains	510:523	mutant strains	510:523	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	3	19	theme	airway	748:753	arg1	recruitment					766:776	increased murine airway eosinophil recruitment	731:776	increased murine airway eosinophil recruitment	731:776	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	4	20	theme	increased	881:889	arg1	transcription					891:903	increased transcription	881:903	increased transcription of IL-17A after a single aspiration	881:939	The response to increased A. fumigatus chitin was associated with increased transcription of IL-17A after a single aspiration, although this cytokine was not required for eosinophil recruitment.
28552410	2	21	theme	core	439:442	arg1	carbohydrates					444:456	cell wall core carbohydrates	429:456	cell wall core carbohydrates β-1,3-glucan to chitin	429:479	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	1	22	theme	fungal	170:175	arg1	polysaccharides					187:201	fungal cell wall polysaccharides	170:201	fungal cell wall polysaccharides that program innate and adaptive immunity	170:243	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	0	23	theme	eosinophil	5:14	arg1	recruitment					16:26	Lung eosinophil recruitment	0:26	Lung eosinophil recruitment in response to Aspergillus fumigatus	0:63	Lung eosinophil recruitment in response to Aspergillus fumigatus is correlated with fungal cell wall composition and requires γδ T cells.
28552410	3	24	theme	eosinophil	755:764	arg1	recruitment					766:776	increased murine airway eosinophil recruitment	731:776	increased murine airway eosinophil recruitment	731:776	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	4	25	theme	IL-17A	908:913	arg1	transcription					891:903	increased transcription	881:903	increased transcription of IL-17A after a single aspiration	881:939	The response to increased A. fumigatus chitin was associated with increased transcription of IL-17A after a single aspiration, although this cytokine was not required for eosinophil recruitment.
28552410	5	26	theme	fungal	1197:1202	arg1	inhalation					1204:1213	fungal inhalation	1197:1213	fungal inhalation	1197:1213	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	2	27	dep	A.	484:485	arg1	fumigatus					487:495	fumigatus	487:495	fumigatus	487:495	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	1	28	theme	cell	177:180	arg1	polysaccharides					187:201	fungal cell wall polysaccharides	170:201	fungal cell wall polysaccharides that program innate and adaptive immunity	170:243	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	0	29	theme	Lung	0:3	arg1	recruitment					16:26	Lung eosinophil recruitment	0:26	Lung eosinophil recruitment in response to Aspergillus fumigatus	0:63	Lung eosinophil recruitment in response to Aspergillus fumigatus is correlated with fungal cell wall composition and requires γδ T cells.
28552410	3	30	theme	drug	694:697	arg1	caspofungin					699:709	the β-1,3-glucan synthesis-targeting drug caspofungin	657:709	the β-1,3-glucan synthesis-targeting drug caspofungin	657:709	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	1	31	theme	wall	182:185	arg1	polysaccharides					187:201	fungal cell wall polysaccharides	170:201	fungal cell wall polysaccharides that program innate and adaptive immunity	170:243	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	2	32	theme	wall	434:437	arg1	carbohydrates					444:456	cell wall core carbohydrates	429:456	cell wall core carbohydrates β-1,3-glucan to chitin	429:479	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	1	33	theme	Aspergillus	288:298	arg1	pathogen					279:286	the human opportunistic fungal pathogen	248:286	the human opportunistic fungal pathogen Aspergillus fumigatus	248:308	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	0	34	theme	γδ	126:127	arg1	cells					131:135	γδ T cells	126:135	γδ T cells	126:135	Lung eosinophil recruitment in response to Aspergillus fumigatus is correlated with fungal cell wall composition and requires γδ T cells.
28552410	1	35	theme	polysaccharides	187:201	arg1	focus					321:325	a focus	319:325	a focus of considerable interest	319:350	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	1	35	theme	polysaccharides	187:201	arg1	recognition					155:165	The differential recognition	138:165	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus	138:308	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	2	36	theme	cell	429:432	arg1	carbohydrates					444:456	cell wall core carbohydrates	429:456	cell wall core carbohydrates β-1,3-glucan to chitin	429:479	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	5	37	theme	type	1170:1173	arg1	responses					1184:1192	potentially detrimental type 2 immune responses	1146:1192	potentially detrimental type 2 immune responses	1146:1192	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	2	38	theme	airway	556:561	arg1	recruitment					574:584	increased airway eosinophil recruitment	546:584	increased airway eosinophil recruitment	546:584	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	5	39	theme	lymphocytes	1101:1111	arg1	subset					1079:1084	this subset	1074:1084	this subset of innate-like lymphocytes	1074:1111	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	5	39	theme	lymphocytes	1101:1111	arg1	regulator					1133:1141	an important regulator	1120:1141	an important regulator of potentially detrimental type 2 immune responses	1120:1192	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	3	40	from	increase	603:610	arg1	exposure					637:644	fungal surface chitin exposure	615:644	fungal surface chitin exposure	615:644	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	5	41	theme	immune	1177:1182	arg1	responses					1184:1192	potentially detrimental type 2 immune responses	1146:1192	potentially detrimental type 2 immune responses	1146:1192	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	3	42	theme	single	786:791	arg1	challenge					793:801	a single challenge	784:801	a single challenge of conidia	784:812	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	2	43	theme	increased	546:554	arg1	recruitment					574:584	increased airway eosinophil recruitment	546:584	increased airway eosinophil recruitment	546:584	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	3	44	theme	β-1,3-glucan	661:672	arg1	caspofungin					699:709	the β-1,3-glucan synthesis-targeting drug caspofungin	657:709	the β-1,3-glucan synthesis-targeting drug caspofungin	657:709	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	0	45	dep	fumigatus	55:63	arg1	response					31:38	response	31:38	response	31:38	Lung eosinophil recruitment in response to Aspergillus fumigatus is correlated with fungal cell wall composition and requires γδ T cells.
28552410	2	46	theme	fungal	373:378	arg1	aspiration					388:397	fungal conidia aspiration	373:397	fungal conidia aspiration	373:397	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	2	47	theme	relative	410:417	arg1	levels					419:424	decreased relative levels	400:424	decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains	400:523	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	5	48	theme	responses	1184:1192	arg1	regulator					1133:1141	an important regulator	1120:1141	an important regulator of potentially detrimental type 2 immune responses	1120:1192	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	5	48	theme	responses	1184:1192	arg1	subset					1079:1084	this subset	1074:1084	this subset of innate-like lymphocytes	1074:1111	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	0	49	theme	T	129:129	arg1	cells					131:135	γδ T cells	126:135	γδ T cells	126:135	Lung eosinophil recruitment in response to Aspergillus fumigatus is correlated with fungal cell wall composition and requires γδ T cells.
28552410	1	50	theme	innate	216:221	arg1	immunity					236:243	program innate and adaptive immunity	208:243	program innate and adaptive immunity	208:243	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	2	51	theme	β-1,3-glucan	458:469	arg1	carbohydrates					444:456	cell wall core carbohydrates	429:456	cell wall core carbohydrates β-1,3-glucan to chitin	429:479	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	3	52	theme	conidia	806:812	arg1	challenge					793:801	a single challenge	784:801	a single challenge of conidia	784:812	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	2	53	theme	eosinophil	563:572	arg1	recruitment					574:584	increased airway eosinophil recruitment	546:584	increased airway eosinophil recruitment	546:584	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	3	54	theme	increased	731:739	arg1	recruitment					766:776	increased murine airway eosinophil recruitment	731:776	increased murine airway eosinophil recruitment	731:776	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	4	55	theme	A.	841:842	arg1	chitin					854:859	increased A. fumigatus chitin	831:859	increased A. fumigatus chitin	831:859	The response to increased A. fumigatus chitin was associated with increased transcription of IL-17A after a single aspiration, although this cytokine was not required for eosinophil recruitment.
28552410	4	56	theme	increased	831:839	arg1	chitin					854:859	increased A. fumigatus chitin	831:859	increased A. fumigatus chitin	831:859	The response to increased A. fumigatus chitin was associated with increased transcription of IL-17A after a single aspiration, although this cytokine was not required for eosinophil recruitment.
28552410	3	57	theme	chitin	630:635	arg1	exposure					637:644	fungal surface chitin exposure	615:644	fungal surface chitin exposure	615:644	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	3	58	theme	synthesis-targeting	674:692	arg1	caspofungin					699:709	the β-1,3-glucan synthesis-targeting drug caspofungin	657:709	the β-1,3-glucan synthesis-targeting drug caspofungin	657:709	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	5	59	theme	T	1035:1035	arg1	cells					1037:1041	both RAG1 and γδ T cells	1018:1041	both RAG1 and γδ T cells	1018:1041	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	1	60	theme	adaptive	227:234	arg1	immunity					236:243	program innate and adaptive immunity	208:243	program innate and adaptive immunity	208:243	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	2	61	theme	decreased	400:408	arg1	levels					419:424	decreased relative levels	400:424	decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains	400:523	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	3	62	theme	fungal	615:620	arg1	exposure					637:644	fungal surface chitin exposure	615:644	fungal surface chitin exposure	615:644	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	4	63	dep	A.	841:842	arg1	fumigatus					844:852	fumigatus	844:852	fumigatus	844:852	The response to increased A. fumigatus chitin was associated with increased transcription of IL-17A after a single aspiration, although this cytokine was not required for eosinophil recruitment.
28552410	2	64	theme	aspiration	388:397	arg1	model					364:368	a mouse model	356:368	a mouse model of fungal conidia aspiration	356:397	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	5	65	theme	γδ	1032:1033	arg1	cells					1037:1041	both RAG1 and γδ T cells	1018:1041	both RAG1 and γδ T cells	1018:1041	Rather, both RAG1 and γδ T cells were required, suggesting that this subset of innate-like lymphocytes may be an important regulator of potentially detrimental type 2 immune responses to fungal inhalation and infection.
28552410	1	66	theme	considerable	330:341	arg1	interest					343:350	considerable interest	330:350	considerable interest	330:350	The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest.
28552410	3	67	theme	surface	622:628	arg1	exposure					637:644	fungal surface chitin exposure	615:644	fungal surface chitin exposure	615:644	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	3	68	theme	murine	741:746	arg1	recruitment					766:776	increased murine airway eosinophil recruitment	731:776	increased murine airway eosinophil recruitment	731:776	In addition, an increase in fungal surface chitin exposure induced by the β-1,3-glucan synthesis-targeting drug caspofungin was associated with increased murine airway eosinophil recruitment after a single challenge of conidia.
28552410	2	69	theme	carbohydrates	444:456	arg1	levels					419:424	decreased relative levels	400:424	decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains	400:523	In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates β-1,3-glucan to chitin in A. fumigatus isolates and mutant strains were correlated with increased airway eosinophil recruitment.
28552410	4	70	theme	eosinophil	986:995	arg1	recruitment					997:1007	eosinophil recruitment	986:1007	eosinophil recruitment	986:1007	The response to increased A. fumigatus chitin was associated with increased transcription of IL-17A after a single aspiration, although this cytokine was not required for eosinophil recruitment.
24746409	8	0	located	observed	1206:1213	arg2	retardation					1190:1200	release retardation	1182:1200	release retardation	1182:1200	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	8	0	located	observed	1206:1213	arg1	order					1227:1231	the same order	1218:1231	the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR)	1218:1299	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	9	1	theme	polymer	1417:1423	arg1	composition					1400:1410	composition	1400:1410	composition	1400:1410	Therefore, the Tg and composition of a polymer need to be considered in polymer design and formulation of controlled-release matrix systems.
24746409	9	1	theme	polymer	1417:1423	arg1	Tg					1393:1394	Tg	1393:1394	Tg	1393:1394	Therefore, the Tg and composition of a polymer need to be considered in polymer design and formulation of controlled-release matrix systems.
24746409	1	2	theme	study	150:154	arg1	objective					132:140	The objective	128:140	The objective of this study	128:154	The objective of this study was to identify and evaluate key polymer properties affecting direct compression and drug release from water-insoluble matrices.
24746409	7	3	theme	higher	1101:1106	arg1	benefits					1122:1129	benefits	1122:1129	benefits of a high-T(g) polymer (ethyl cellulose)	1122:1170	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	7	3	theme	higher	1101:1106	arg1	permeability					1084:1095	lower permeability	1078:1095	lower permeability	1078:1095	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	7	3	theme	higher	1101:1106	arg1	stability					1108:1116	higher stability	1101:1116	higher stability	1101:1116	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	0	4	from	effect	4:9	arg1	release					64:70	drug release	59:70	drug release	59:70	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	0	4	from	effect	4:9	arg1	compression					43:53	direct compression	36:53	direct compression	36:53	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	9	5	theme	polymer	1450:1456	arg1	design					1458:1463	polymer design	1450:1463	polymer design	1450:1463	Therefore, the Tg and composition of a polymer need to be considered in polymer design and formulation of controlled-release matrix systems.
24746409	4	6	theme	surface	628:634	arg1	properties					636:645	the surface properties	624:645	the surface properties	624:645	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	4	6	theme	surface	628:634	arg1	parameters					573:582	Key parameters	569:582	Key parameters influencing polymer compactibility	569:617	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	6	7	theme	Kollidon	895:902	arg1	RS					922:923	Kollidon(®) SR<Eudragit(®) RS	895:923	Kollidon(®) SR<Eudragit(®) RS	895:923	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	6	7	theme	Kollidon	895:902	arg1	T					889:889	A low T	883:889	A low T(g) (Kollidon(®) SR<Eudragit(®) RS)	883:924	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	0	8	theme	controlled	93:102	arg1	tablets					119:125	water-insoluble controlled release matrix tablets	77:125	water-insoluble controlled release matrix tablets	77:125	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	8	9	theme	®	1263:1263	arg1	integrity					1243:1251	matrix integrity	1236:1251	matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR)	1236:1299	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	8	9	theme	®	1263:1263	arg1	SR					1297:1298	Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR	1254:1298	Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR	1254:1298	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	3	10	theme	similar	482:488	arg1	threshold					507:515	A similar site percolation threshold	480:515	A similar site percolation threshold of 65% v/v	480:526	A similar site percolation threshold of 65% v/v was found for all polymers in dry state.
24746409	1	11	theme	water-insoluble	259:273	arg1	matrices					275:282	water-insoluble matrices	259:282	water-insoluble matrices	259:282	The objective of this study was to identify and evaluate key polymer properties affecting direct compression and drug release from water-insoluble matrices.
24746409	2	12	theme	wet	469:471	arg1	state					473:477	dry and wet state	461:477	dry and wet state	461:477	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	3	13	theme	site	490:493	arg1	threshold					507:515	A similar site percolation threshold	480:515	A similar site percolation threshold of 65% v/v	480:526	A similar site percolation threshold of 65% v/v was found for all polymers in dry state.
24746409	2	14	theme	used	294:297	arg1	polymers					299:306	Commonly used polymers	285:306	Commonly used polymers	285:306	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	2	14	theme	used	294:297	arg1	RS					345:346	Kollidon(®) SR, Eudragit(®) RS	317:346	RS	345:346	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	2	14	theme	used	294:297	arg1	cellulose					358:366	ethyl cellulose	352:366	ethyl cellulose	352:366	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	4	15	dep	temperature	672:682	arg1	T					685:685	T	685:685	T(g)	685:688	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	4	15	dep	temperature	672:682	arg1	g					687:687	g	687:687	g	687:687	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	0	16	theme	matrix	112:117	arg1	tablets					119:125	water-insoluble controlled release matrix tablets	77:125	water-insoluble controlled release matrix tablets	77:125	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	6	17	theme	particle	963:970	arg1	size					972:975	particle size effect and tortuosity	963:997	size	972:975	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	6	17	theme	particle	963:970	arg1	threshold					952:960	the percolation threshold	936:960	the percolation threshold	936:960	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	5	18	theme	important	762:770	arg1	properties					772:781	The important properties	758:781	The important properties observed in dry state	758:803	The important properties observed in dry state also governed matrix characteristics and therefore drug release in wet state.
24746409	6	19	theme	heat/humidity	1046:1058	arg1	treatment					1060:1068	heat/humidity treatment	1046:1068	heat/humidity treatment	1046:1068	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	2	20	theme	ethyl	352:356	arg1	cellulose					358:366	ethyl cellulose	352:366	ethyl cellulose	352:366	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	0	21	theme	release	104:110	arg1	tablets					119:125	water-insoluble controlled release matrix tablets	77:125	water-insoluble controlled release matrix tablets	77:125	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	5	22	theme	wet	872:874	arg1	state					876:880	wet state	872:880	wet state	872:880	The important properties observed in dry state also governed matrix characteristics and therefore drug release in wet state.
24746409	3	23	from	polymers	546:553	arg1	state					562:566	dry state	558:566	dry state	558:566	A similar site percolation threshold of 65% v/v was found for all polymers in dry state.
24746409	5	24	theme	dry	795:797	arg1	state					799:803	dry state	795:803	dry state	795:803	The important properties observed in dry state also governed matrix characteristics and therefore drug release in wet state.
24746409	8	25	theme	Kollidon	1362:1369	arg1	SR					1374:1375	Kollidon(®) SR	1362:1375	Kollidon(®) SR	1362:1375	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	8	26	theme	matrix	1236:1241	arg1	integrity					1243:1251	matrix integrity	1236:1251	matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR)	1236:1299	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	8	26	theme	matrix	1236:1241	arg1	SR					1297:1298	Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR	1254:1298	Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR	1254:1298	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	2	27	theme	Kollidon	317:324	arg1	RS					345:346	Kollidon(®) SR, Eudragit(®) RS	317:346	RS	345:346	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	7	28	theme	g	1143:1143	arg1	cellulose					1161:1169	a high-T(g) polymer (ethyl cellulose)	1134:1170	a high-T(g) polymer (ethyl cellulose)	1134:1170	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	0	29	theme	water-insoluble	77:91	arg1	tablets					119:125	water-insoluble controlled release matrix tablets	77:125	water-insoluble controlled release matrix tablets	77:125	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	7	30	theme	polymer	1146:1152	arg1	cellulose					1161:1169	a high-T(g) polymer (ethyl cellulose)	1134:1170	a high-T(g) polymer (ethyl cellulose)	1134:1170	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	9	31	theme	controlled-release	1484:1501	arg1	systems					1510:1516	controlled-release matrix systems	1484:1516	controlled-release matrix systems	1484:1516	Therefore, the Tg and composition of a polymer need to be considered in polymer design and formulation of controlled-release matrix systems.
24746409	3	32	theme	65	520:521	arg1	%					522:522	%	522:522	%	522:522	A similar site percolation threshold of 65% v/v was found for all polymers in dry state.
24746409	4	33	theme	polymer	596:602	arg1	compactibility					604:617	polymer compactibility	596:617	polymer compactibility	596:617	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	0	34	theme	polymer	14:20	arg1	properties					22:31	polymer properties	14:31	polymer properties	14:31	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	6	35	dep	size	972:975	arg1	effect					977:982	effect	977:982	effect	977:982	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	3	36	theme	percolation	495:505	arg1	threshold					507:515	A similar site percolation threshold	480:515	A similar site percolation threshold of 65% v/v	480:526	A similar site percolation threshold of 65% v/v was found for all polymers in dry state.
24746409	1	37	theme	key	185:187	arg1	properties					197:206	key polymer properties	185:206	key polymer properties affecting direct compression and drug release from water-insoluble matrices	185:282	The objective of this study was to identify and evaluate key polymer properties affecting direct compression and drug release from water-insoluble matrices.
24746409	8	38	theme	same	1222:1225	arg1	order					1227:1231	the same order	1218:1231	the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR)	1218:1299	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	6	39	theme	®	904:904	arg1	RS					922:923	Kollidon(®) SR<Eudragit(®) RS	895:923	Kollidon(®) SR<Eudragit(®) RS	895:923	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	6	39	theme	®	904:904	arg1	T					889:889	A low T	883:889	A low T(g) (Kollidon(®) SR<Eudragit(®) RS)	883:924	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	1	40	theme	polymer	189:195	arg1	properties					197:206	key polymer properties	185:206	key polymer properties affecting direct compression and drug release from water-insoluble matrices	185:282	The objective of this study was to identify and evaluate key polymer properties affecting direct compression and drug release from water-insoluble matrices.
24746409	7	41	theme	ethyl	1155:1159	arg1	cellulose					1161:1169	a high-T(g) polymer (ethyl cellulose)	1134:1170	a high-T(g) polymer (ethyl cellulose)	1134:1170	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	6	42	theme	low	885:887	arg1	RS					922:923	Kollidon(®) SR<Eudragit(®) RS	895:923	Kollidon(®) SR<Eudragit(®) RS	895:923	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	6	42	theme	low	885:887	arg1	T					889:889	A low T	883:889	A low T(g) (Kollidon(®) SR<Eudragit(®) RS)	883:924	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	6	42	theme	low	885:887	arg1	g					891:891	g	891:891	g	891:891	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	8	43	theme	cellulose<Kollidon	1275:1292	arg1	integrity					1243:1251	matrix integrity	1236:1251	matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR)	1236:1299	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	8	43	theme	cellulose<Kollidon	1275:1292	arg1	SR					1297:1298	Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR	1254:1298	Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR	1254:1298	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	4	44	theme	size-dependent	734:747	arg1	binding					749:755	particle size-dependent binding	725:755	particle size-dependent binding	725:755	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	4	45	theme	Key	569:571	arg1	properties					636:645	the surface properties	624:645	the surface properties	624:645	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	4	45	theme	Key	569:571	arg1	parameters					573:582	Key parameters	569:582	Key parameters influencing polymer compactibility	569:617	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	0	46	theme	properties	22:31	arg1	effect					4:9	The effect	0:9	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.	0:126	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	8	47	theme	®	1371:1371	arg1	SR					1374:1375	Kollidon(®) SR	1362:1375	Kollidon(®) SR	1362:1375	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	3	48	theme	dry	558:560	arg1	state					562:566	dry state	558:566	dry state	558:566	A similar site percolation threshold of 65% v/v was found for all polymers in dry state.
24746409	4	49	theme	particle	725:732	arg1	binding					749:755	particle size-dependent binding	725:755	particle size-dependent binding	725:755	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	2	50	theme	dry	461:463	arg1	state					473:477	dry and wet state	461:477	dry and wet state	461:477	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	7	51	theme	lower	1078:1082	arg1	benefits					1122:1129	benefits	1122:1129	benefits of a high-T(g) polymer (ethyl cellulose)	1122:1170	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	7	51	theme	lower	1078:1082	arg1	permeability					1084:1095	lower permeability	1078:1095	lower permeability	1078:1095	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	7	51	theme	lower	1078:1082	arg1	stability					1108:1116	higher stability	1101:1116	higher stability	1101:1116	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	9	52	theme	matrix	1503:1508	arg1	systems					1510:1516	controlled-release matrix systems	1484:1516	controlled-release matrix systems	1484:1516	Therefore, the Tg and composition of a polymer need to be considered in polymer design and formulation of controlled-release matrix systems.
24746409	2	53	from	properties	447:456	arg1	state					473:477	dry and wet state	461:477	dry and wet state	461:477	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	3	54	theme	%	522:522	arg1	v/v					524:526	65% v/v	520:526	65% v/v	520:526	A similar site percolation threshold of 65% v/v was found for all polymers in dry state.
24746409	2	55	theme	SR	329:330	arg1	RS					345:346	Kollidon(®) SR, Eudragit(®) RS	317:346	RS	345:346	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	0	56	theme	direct	36:41	arg1	compression					43:53	direct compression	36:53	direct compression	36:53	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	8	57	theme	Eudragit	1254:1261	arg1	integrity					1243:1251	matrix integrity	1236:1251	matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR)	1236:1299	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	8	57	theme	Eudragit	1254:1261	arg1	SR					1297:1298	Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR	1254:1298	Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR	1254:1298	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	9	58	theme	systems	1510:1516	arg1	formulation					1469:1479	formulation	1469:1479	formulation	1469:1479	Therefore, the Tg and composition of a polymer need to be considered in polymer design and formulation of controlled-release matrix systems.
24746409	9	58	theme	systems	1510:1516	arg1	design					1458:1463	polymer design	1450:1463	polymer design	1450:1463	Therefore, the Tg and composition of a polymer need to be considered in polymer design and formulation of controlled-release matrix systems.
24746409	3	59	theme	v/v	524:526	arg1	threshold					507:515	A similar site percolation threshold	480:515	A similar site percolation threshold of 65% v/v	480:526	A similar site percolation threshold of 65% v/v was found for all polymers in dry state.
24746409	5	60	theme	drug	856:859	arg1	release					861:867	drug release	856:867	drug release	856:867	The important properties observed in dry state also governed matrix characteristics and therefore drug release in wet state.
24746409	7	61	theme	high-T	1136:1141	arg1	cellulose					1161:1169	a high-T(g) polymer (ethyl cellulose)	1134:1170	a high-T(g) polymer (ethyl cellulose)	1134:1170	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	4	62	theme	polymer	702:708	arg1	elasticity					710:719	polymer elasticity	702:719	polymer elasticity	702:719	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	2	63	dep	RS	345:346	arg1	Eudragit					333:340	Kollidon(®) SR, Eudragit(®) RS	317:346	Eudragit	333:340	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	6	64	theme	SR<Eudragit	907:917	arg1	RS					922:923	Kollidon(®) SR<Eudragit(®) RS	895:923	Kollidon(®) SR<Eudragit(®) RS	895:923	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	6	64	theme	SR<Eudragit	907:917	arg1	T					889:889	A low T	883:889	A low T(g) (Kollidon(®) SR<Eudragit(®) RS)	883:924	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	2	65	theme	®	326:326	arg1	RS					345:346	Kollidon(®) SR, Eudragit(®) RS	317:346	RS	345:346	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	1	66	theme	direct	218:223	arg1	compression					225:235	direct compression	218:235	direct compression	218:235	The objective of this study was to identify and evaluate key polymer properties affecting direct compression and drug release from water-insoluble matrices.
24746409	5	67	located	observed	783:790	arg1	state					799:803	dry state	795:803	dry state	795:803	The important properties observed in dry state also governed matrix characteristics and therefore drug release in wet state.
24746409	5	67	located	observed	783:790	arg2	properties					772:781	The important properties	758:781	The important properties observed in dry state	758:803	The important properties observed in dry state also governed matrix characteristics and therefore drug release in wet state.
24746409	4	68	theme	glass	655:659	arg1	transition					661:670	the glass transition	651:670	the glass transition temperature (T(g))	651:689	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	8	69	theme	release	1182:1188	arg1	retardation					1190:1200	release retardation	1182:1200	release retardation	1182:1200	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	4	70	theme	transition	661:670	arg1	temperature					672:682	the glass transition temperature	651:682	the glass transition temperature (T(g))	651:689	Key parameters influencing polymer compactibility were the surface properties and the glass transition temperature (T(g)), affecting polymer elasticity and particle size-dependent binding.
24746409	0	71	theme	drug	59:62	arg1	release					64:70	drug release	59:70	drug release	59:70	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	6	72	theme	®	919:919	arg1	RS					922:923	Kollidon(®) SR<Eudragit(®) RS	895:923	Kollidon(®) SR<Eudragit(®) RS	895:923	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	6	72	theme	®	919:919	arg1	T					889:889	A low T	883:889	A low T(g) (Kollidon(®) SR<Eudragit(®) RS)	883:924	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	8	73	theme	RS<ethyl	1266:1273	arg1	integrity					1243:1251	matrix integrity	1236:1251	matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR)	1236:1299	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	8	73	theme	RS<ethyl	1266:1273	arg1	SR					1297:1298	Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR	1254:1298	Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR	1254:1298	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	7	74	theme	cellulose	1161:1169	arg1	benefits					1122:1129	benefits	1122:1129	benefits of a high-T(g) polymer (ethyl cellulose)	1122:1170	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	7	74	theme	cellulose	1161:1169	arg1	permeability					1084:1095	lower permeability	1078:1095	lower permeability	1078:1095	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	7	74	theme	cellulose	1161:1169	arg1	stability					1108:1116	higher stability	1101:1116	higher stability	1101:1116	Hence, lower permeability and higher stability are benefits of a high-T(g) polymer (ethyl cellulose).
24746409	2	75	theme	®	342:342	arg1	RS					345:346	Kollidon(®) SR, Eudragit(®) RS	317:346	RS	345:346	Commonly used polymers, such as Kollidon(®) SR, Eudragit(®) RS and ethyl cellulose, were characterized, formulated into tablets and compared with regard to their properties in dry and wet state.
24746409	6	76	theme	percolation	940:950	arg1	size					972:975	particle size effect and tortuosity	963:997	size	972:975	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	6	76	theme	percolation	940:950	arg1	tortuosity					988:997	tortuosity	988:997	tortuosity	988:997	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	6	76	theme	percolation	940:950	arg1	threshold					952:960	the percolation threshold	936:960	the percolation threshold	936:960	A low T(g) (Kollidon(®) SR<Eudragit(®) RS) decreased the percolation threshold, particle size effect and tortuosity, but increased permeability and sensitivity to heat/humidity treatment.
24746409	8	77	theme	high	1309:1312	arg1	permeability					1314:1325	the high permeability	1305:1325	the high permeability	1305:1325	However, release retardation was observed in the same order as matrix integrity (Eudragit(®) RS<ethyl cellulose<Kollidon(®) SR), as the high permeability was counteracted by PVP in case of Kollidon(®) SR.
24746409	0	78	from	tablets	119:125	arg1	release					64:70	drug release	59:70	drug release	59:70	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	0	78	from	tablets	119:125	arg1	compression					43:53	direct compression	36:53	direct compression	36:53	The effect of polymer properties on direct compression and drug release from water-insoluble controlled release matrix tablets.
24746409	5	79	theme	matrix	819:824	arg1	characteristics					826:840	matrix characteristics	819:840	matrix characteristics	819:840	The important properties observed in dry state also governed matrix characteristics and therefore drug release in wet state.
24746409	1	80	theme	drug	241:244	arg1	release					246:252	drug release	241:252	drug release	241:252	The objective of this study was to identify and evaluate key polymer properties affecting direct compression and drug release from water-insoluble matrices.
28986683	0	0	theme	loading	93:99	arg1	studies					101:107	their drug loading studies	82:107	their drug loading studies	82:107	Hydroxyapatite-chitosan biocomposites synthesized in the simulated body fluid and their drug loading studies.
28986683	13	1	theme	produced	1433:1440	arg1	material					1442:1449	This new produced material	1424:1449	This new produced material	1424:1449	This new produced material can be applicable for drug delivery.
28986683	2	2	theme	potential	315:323	arg1	use					325:327	its potential use	311:327	its potential use in the drug delivery systems	311:356	Chitosan (CTS), a natural polymer, is another material has been widely investigated for its potential use in the drug delivery systems.
28986683	11	3	theme	crosslinking	1312:1323	arg1	agent					1325:1329	crosslinking agent	1312:1329	crosslinking agent	1312:1329	It is observed that weight ratio of polymer and crosslinking agent can be manipulated to adjust drug loading.
28986683	0	4	theme	drug	88:91	arg1	studies					101:107	their drug loading studies	82:107	their drug loading studies	82:107	Hydroxyapatite-chitosan biocomposites synthesized in the simulated body fluid and their drug loading studies.
28986683	8	5	theme	load	887:890	arg1	efficiency					892:901	the greatest drug load efficiency	869:901	the greatest drug load efficiency	869:901	The weight ratio giving the greatest drug load efficiency is selected for the last step of the study.
28986683	7	6	theme	1:2	821:823	arg1	ratios					806:811	the ratios	802:811	the ratios of 1:1, 1:2 and 1:4 of HAp-CTS	802:842	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	7	7	theme	HAp-CTS	836:842	arg1	1:1					816:818	1:1	816:818	1:1	816:818	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	7	7	theme	HAp-CTS	836:842	arg1	HAp-CTS					836:842	HAp-CTS	836:842	HAp-CTS	836:842	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	13	8	theme	new	1429:1431	arg1	material					1442:1449	This new produced material	1424:1449	This new produced material	1424:1449	This new produced material can be applicable for drug delivery.
28986683	11	9	theme	polymer	1300:1306	arg1	ratio					1291:1295	weight ratio	1284:1295	weight ratio of polymer and crosslinking agent	1284:1329	It is observed that weight ratio of polymer and crosslinking agent can be manipulated to adjust drug loading.
28986683	9	10	theme	drug	1125:1128	arg1	studies					1138:1144	drug release studies	1125:1144	drug release studies	1125:1144	Crosslinking agent, glutaraldehyde, are changed from 0 to 5% on the selected sample, then, drug loading is examined again in various environment owing different pH. Furthermore, drug release studies are conducted.
28986683	9	11	theme	selected	1015:1022	arg1	sample					1024:1029	the selected sample	1011:1029	the selected sample	1011:1029	Crosslinking agent, glutaraldehyde, are changed from 0 to 5% on the selected sample, then, drug loading is examined again in various environment owing different pH. Furthermore, drug release studies are conducted.
28986683	3	12	theme	drug	443:446	arg1	loading					448:454	their drug loading	437:454	their drug loading	437:454	In this study, the composites of HAp-CTS are produced in order to investigate their drug loading and release studies.
28986683	10	13	dep	structure	1179:1187	arg1	the					1175:1177	the	1175:1177	the	1175:1177	To understand the structure and morphology of the samples, XRD, FTIR, SEM and Uv-Spectrum are applied.
28986683	9	14	dep	5	1005:1005	arg1	to					1002:1003	to	1002:1003	to	1002:1003	Crosslinking agent, glutaraldehyde, are changed from 0 to 5% on the selected sample, then, drug loading is examined again in various environment owing different pH. Furthermore, drug release studies are conducted.
28986683	9	15	theme	release	1130:1136	arg1	studies					1138:1144	drug release studies	1125:1144	drug release studies	1125:1144	Crosslinking agent, glutaraldehyde, are changed from 0 to 5% on the selected sample, then, drug loading is examined again in various environment owing different pH. Furthermore, drug release studies are conducted.
28986683	7	16	theme	1:4	829:831	arg1	ratios					806:811	the ratios	802:811	the ratios of 1:1, 1:2 and 1:4 of HAp-CTS	802:842	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	2	17	theme	natural	241:247	arg1	polymer					249:255	a natural polymer	239:255	a natural polymer	239:255	Chitosan (CTS), a natural polymer, is another material has been widely investigated for its potential use in the drug delivery systems.
28986683	2	17	theme	natural	241:247	arg1	Chitosan					223:230	Chitosan	223:230	Chitosan (CTS)	223:236	Chitosan (CTS), a natural polymer, is another material has been widely investigated for its potential use in the drug delivery systems.
28986683	7	18	theme	5-Fluorouracil	767:780	arg1	effect					741:746	its effect	737:746	its effect on drug loading of 5-Fluorouracil, a cancer drug,	737:796	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	1	19	theme	biomedical	162:171	arg1	matrices					188:195	matrices	188:195	matrices for drug release control	188:220	Hydroxyapatite (HAp) is a bioceramic applied in the biomedical areas, such as matrices for drug release control.
28986683	1	19	theme	biomedical	162:171	arg1	areas					173:177	the biomedical areas	158:177	the biomedical areas	158:177	Hydroxyapatite (HAp) is a bioceramic applied in the biomedical areas, such as matrices for drug release control.
28986683	13	20	theme	drug	1473:1476	arg1	delivery					1478:1485	drug delivery	1473:1485	drug delivery	1473:1485	This new produced material can be applicable for drug delivery.
28986683	11	21	theme	drug	1360:1363	arg1	loading					1365:1371	drug loading	1360:1371	drug loading	1360:1371	It is observed that weight ratio of polymer and crosslinking agent can be manipulated to adjust drug loading.
28986683	7	22	theme	drug	751:754	arg1	loading					756:762	drug loading	751:762	drug loading	751:762	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	2	23	theme	delivery	341:348	arg1	systems					350:356	the drug delivery systems	332:356	the drug delivery systems	332:356	Chitosan (CTS), a natural polymer, is another material has been widely investigated for its potential use in the drug delivery systems.
28986683	9	24	dep	pH.	1108:1110	arg1	conducted					1150:1158	conducted	1150:1158	are conducted	1146:1158	Crosslinking agent, glutaraldehyde, are changed from 0 to 5% on the selected sample, then, drug loading is examined again in various environment owing different pH. Furthermore, drug release studies are conducted.
28986683	7	25	theme	1:1	816:818	arg1	ratios					806:811	the ratios	802:811	the ratios of 1:1, 1:2 and 1:4 of HAp-CTS	802:842	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	2	26	theme	drug	336:339	arg1	systems					350:356	the drug delivery systems	332:356	the drug delivery systems	332:356	Chitosan (CTS), a natural polymer, is another material has been widely investigated for its potential use in the drug delivery systems.
28986683	8	27	theme	last	923:926	arg1	step					928:931	the last step	919:931	the last step of the study	919:944	The weight ratio giving the greatest drug load efficiency is selected for the last step of the study.
28986683	8	28	theme	drug	882:885	arg1	efficiency					892:901	the greatest drug load efficiency	869:901	the greatest drug load efficiency	869:901	The weight ratio giving the greatest drug load efficiency is selected for the last step of the study.
28986683	9	29	theme	Crosslinking	947:958	arg1	agent					960:964	Crosslinking agent	947:964	Crosslinking agent	947:964	Crosslinking agent, glutaraldehyde, are changed from 0 to 5% on the selected sample, then, drug loading is examined again in various environment owing different pH. Furthermore, drug release studies are conducted.
28986683	9	29	theme	Crosslinking	947:958	arg1	glutaraldehyde					967:980	glutaraldehyde	967:980	glutaraldehyde	967:980	Crosslinking agent, glutaraldehyde, are changed from 0 to 5% on the selected sample, then, drug loading is examined again in various environment owing different pH. Furthermore, drug release studies are conducted.
28986683	4	30	theme	all	486:488	arg1	First					477:481	First	477:481	First	477:481	First of all, HAp-CTS composites are produced in the presence of simulated body fluid (SBF).
28986683	12	31	theme	Fickian	1405:1411	arg1	diffusion					1413:1421	the Fickian diffusion	1401:1421	the Fickian diffusion	1401:1421	Release kinetics are shown the Fickian diffusion.
28986683	2	32	from	use	325:327	arg1	systems					350:356	the drug delivery systems	332:356	the drug delivery systems	332:356	Chitosan (CTS), a natural polymer, is another material has been widely investigated for its potential use in the drug delivery systems.
28986683	7	33	theme	CTS	712:714	arg1	ratio					703:707	The weight ratio	692:707	The weight ratio of CTS	692:714	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	7	33	theme	CTS	712:714	arg1	varied					719:724	varied	719:724	varied	719:724	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	4	34	theme	fluid	557:561	arg1	presence					530:537	the presence	526:537	the presence of simulated body fluid (SBF)	526:567	First of all, HAp-CTS composites are produced in the presence of simulated body fluid (SBF).
28986683	0	35	theme	simulated	57:65	arg1	fluid					72:76	the simulated body fluid	53:76	the simulated body fluid	53:76	Hydroxyapatite-chitosan biocomposites synthesized in the simulated body fluid and their drug loading studies.
28986683	4	36	theme	HAp-CTS	491:497	arg1	composites					499:508	HAp-CTS composites	491:508	HAp-CTS composites	491:508	First of all, HAp-CTS composites are produced in the presence of simulated body fluid (SBF).
28986683	3	37	theme	HAp-CTS	392:398	arg1	composites					378:387	the composites	374:387	the composites of HAp-CTS	374:398	In this study, the composites of HAp-CTS are produced in order to investigate their drug loading and release studies.
28986683	3	37	theme	HAp-CTS	392:398	arg1	HAp-CTS					392:398	HAp-CTS	392:398	HAp-CTS	392:398	In this study, the composites of HAp-CTS are produced in order to investigate their drug loading and release studies.
28986683	12	38	theme	Release	1374:1380	arg1	kinetics					1382:1389	Release kinetics	1374:1389	Release kinetics	1374:1389	Release kinetics are shown the Fickian diffusion.
28986683	8	39	theme	study	940:944	arg1	step					928:931	the last step	919:931	the last step of the study	919:944	The weight ratio giving the greatest drug load efficiency is selected for the last step of the study.
28986683	8	40	theme	greatest	873:880	arg1	efficiency					892:901	the greatest drug load efficiency	869:901	the greatest drug load efficiency	869:901	The weight ratio giving the greatest drug load efficiency is selected for the last step of the study.
28986683	1	41	theme	drug	201:204	arg1	control					214:220	drug release control	201:220	drug release control	201:220	Hydroxyapatite (HAp) is a bioceramic applied in the biomedical areas, such as matrices for drug release control.
28986683	7	42	from	effect	741:746	arg1	loading					756:762	drug loading	751:762	drug loading	751:762	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	9	43	theme	drug	1038:1041	arg1	loading					1043:1049	drug loading	1038:1049	drug loading	1038:1049	Crosslinking agent, glutaraldehyde, are changed from 0 to 5% on the selected sample, then, drug loading is examined again in various environment owing different pH. Furthermore, drug release studies are conducted.
28986683	0	44	theme	body	67:70	arg1	fluid					72:76	the simulated body fluid	53:76	the simulated body fluid	53:76	Hydroxyapatite-chitosan biocomposites synthesized in the simulated body fluid and their drug loading studies.
28986683	11	45	theme	weight	1284:1289	arg1	ratio					1291:1295	weight ratio	1284:1295	weight ratio of polymer and crosslinking agent	1284:1329	It is observed that weight ratio of polymer and crosslinking agent can be manipulated to adjust drug loading.
28986683	7	46	theme	weight	696:701	arg1	ratio					703:707	The weight ratio	692:707	The weight ratio of CTS	692:714	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	7	46	theme	weight	696:701	arg1	varied					719:724	varied	719:724	varied	719:724	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	5	47	theme	HAp-CTS	606:612	arg1	composites					614:623	HAp-CTS composites	606:623	HAp-CTS composites	606:623	Analysis confirmed the structure of HAp-CTS composites.
28986683	9	48	theme	various	1072:1078	arg1	environment					1080:1090	various environment	1072:1090	various environment owing different pH. Furthermore, drug release studies are conducted	1072:1158	Crosslinking agent, glutaraldehyde, are changed from 0 to 5% on the selected sample, then, drug loading is examined again in various environment owing different pH. Furthermore, drug release studies are conducted.
28986683	11	49	theme	agent	1325:1329	arg1	ratio					1291:1295	weight ratio	1284:1295	weight ratio of polymer and crosslinking agent	1284:1329	It is observed that weight ratio of polymer and crosslinking agent can be manipulated to adjust drug loading.
28986683	10	50	theme	samples	1211:1217	arg1	structure					1179:1187	structure	1179:1187	structure	1179:1187	To understand the structure and morphology of the samples, XRD, FTIR, SEM and Uv-Spectrum are applied.
28986683	10	50	theme	samples	1211:1217	arg1	morphology					1193:1202	morphology	1193:1202	morphology	1193:1202	To understand the structure and morphology of the samples, XRD, FTIR, SEM and Uv-Spectrum are applied.
28986683	8	51	theme	weight	849:854	arg1	ratio					856:860	The weight ratio	845:860	The weight ratio giving the greatest drug load efficiency	845:901	The weight ratio giving the greatest drug load efficiency is selected for the last step of the study.
28986683	1	52	theme	release	206:212	arg1	control					214:220	drug release control	201:220	drug release control	201:220	Hydroxyapatite (HAp) is a bioceramic applied in the biomedical areas, such as matrices for drug release control.
28986683	5	53	theme	composites	614:623	arg1	structure					593:601	the structure	589:601	the structure of HAp-CTS composites	589:623	Analysis confirmed the structure of HAp-CTS composites.
28986683	2	54	dep	material	269:276	arg1	investigated					294:305	investigated	294:305	has been widely investigated for its potential use in the drug delivery systems	278:356	Chitosan (CTS), a natural polymer, is another material has been widely investigated for its potential use in the drug delivery systems.
28986683	0	55	dep	biocomposites	24:36	arg1	synthesized					38:48	synthesized	38:48	biocomposites synthesized in the simulated body fluid and their drug loading studies	24:107	Hydroxyapatite-chitosan biocomposites synthesized in the simulated body fluid and their drug loading studies.
28986683	9	56	from	%	1006:1006	arg1	sample					1024:1029	the selected sample	1011:1029	the selected sample	1011:1029	Crosslinking agent, glutaraldehyde, are changed from 0 to 5% on the selected sample, then, drug loading is examined again in various environment owing different pH. Furthermore, drug release studies are conducted.
28986683	4	57	theme	body	552:555	arg1	SBF					564:566	SBF	564:566	SBF	564:566	First of all, HAp-CTS composites are produced in the presence of simulated body fluid (SBF).
28986683	4	57	theme	body	552:555	arg1	fluid					557:561	simulated body fluid	542:561	simulated body fluid (SBF)	542:567	First of all, HAp-CTS composites are produced in the presence of simulated body fluid (SBF).
28986683	7	58	theme	cancer	785:790	arg1	drug					792:795	a cancer drug	783:795	a cancer drug	783:795	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	7	58	theme	cancer	785:790	arg1	5-Fluorouracil					767:780	5-Fluorouracil	767:780	5-Fluorouracil	767:780	The weight ratio of CTS is varied to realize its effect on drug loading of 5-Fluorouracil, a cancer drug, for the ratios of 1:1, 1:2 and 1:4 of HAp-CTS.
28986683	4	59	theme	simulated	542:550	arg1	SBF					564:566	SBF	564:566	SBF	564:566	First of all, HAp-CTS composites are produced in the presence of simulated body fluid (SBF).
28986683	4	59	theme	simulated	542:550	arg1	fluid					557:561	simulated body fluid	542:561	simulated body fluid (SBF)	542:567	First of all, HAp-CTS composites are produced in the presence of simulated body fluid (SBF).
23852919	6	0	theme	treated	910:916	arg1	cells					918:922	treated cells	910:922	treated cells	910:922	Elevation in reactive oxygen species level indicates induction of oxidative stress in treated cells.
23852919	9	1	from	concentration	1240:1252	arg1	induction					1210:1218	The induction	1206:1218	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs	1206:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	6	2	theme	stress	900:905	arg1	induction					877:885	induction	877:885	induction of oxidative stress in treated cells	877:922	Elevation in reactive oxygen species level indicates induction of oxidative stress in treated cells.
23852919	4	3	theme	refractory	691:700	arg1	U87MG					702:706	refractory U87MG	691:706	refractory U87MG (human glioblastoma) cells	691:733	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	4	3	theme	refractory	691:700	arg1	glioblastoma					715:726	human glioblastoma	709:726	human glioblastoma	709:726	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	1	4	theme	nanoparticles	193:205	arg1	NC					279:280	Ag NPs-Alg-Chi NC	264:280	Ag NPs-Alg-Chi NC	264:280	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	1	4	theme	nanoparticles	193:205	arg1	nanocarrier					251:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier	164:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC)	164:281	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	8	5	theme	TUNEL	1193:1197	arg1	assay					1199:1203	TUNEL assay	1193:1203	TUNEL assay	1193:1203	Fluorescence and SEM images of the treated cells reveal nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay.
23852919	7	6	from	depolarization	985:998	arg1	evident					968:974	evident	968:974	evident	968:974	Mitochondrial dysfunction in cell death is evident from the depolarization of mitochondrial membrane potential (ΔΨm ).
23852919	4	7	from	effect	681:686	arg1	cells					729:733	refractory U87MG (human glioblastoma) cells	691:733	refractory U87MG (human glioblastoma) cells	691:733	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	1	8	theme	NPs	208:210	arg1	NC					279:280	Ag NPs-Alg-Chi NC	264:280	Ag NPs-Alg-Chi NC	264:280	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	1	8	theme	NPs	208:210	arg1	nanocarrier					251:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier	164:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC)	164:281	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	4	9	theme	anticell	658:665	arg1	effect					681:686	anticell proliferative effect	658:686	anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs	658:769	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	2	10	theme	NPs-Alg-Chi	316:326	arg1	NC					328:329	Ag NPs-Alg-Chi NC	313:329	Ag NPs-Alg-Chi NC	313:329	The synthesis of Ag NPs-Alg-Chi NC is based on the polyelectrolyte complex formation between alginate and chitosan.
23852919	1	11	theme	impregnated	213:223	arg1	NC					279:280	Ag NPs-Alg-Chi NC	264:280	Ag NPs-Alg-Chi NC	264:280	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	1	11	theme	impregnated	213:223	arg1	nanocarrier					251:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier	164:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC)	164:281	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	9	12	from	NC	1290:1291	arg1	present					1264:1270	present	1264:1270	present	1264:1270	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	9	12	from	NC	1290:1291	arg1	comparison					1296:1305	comparison	1296:1305	comparison with free Ag NPs	1296:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	5	13	theme	extensive	802:810	arg1	damage					816:821	extensive DNA damage	802:821	extensive DNA damage	802:821	The cell cycle analysis shows extensive DNA damage.
23852919	7	14	theme	potential	1026:1034	arg1	depolarization					985:998	the depolarization	981:998	the depolarization of mitochondrial membrane potential (ΔΨm )	981:1041	Mitochondrial dysfunction in cell death is evident from the depolarization of mitochondrial membrane potential (ΔΨm ).
23852919	1	15	theme	nanocarrier	251:261	arg1	development					149:159	the development	145:159	the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC)	145:281	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	6	16	from	Elevation	824:832	arg1	level					861:865	reactive oxygen species level	837:865	reactive oxygen species level	837:865	Elevation in reactive oxygen species level indicates induction of oxidative stress in treated cells.
23852919	9	17	theme	Ag	1257:1258	arg1	NPs					1260:1262	Ag NPs	1257:1262	Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs	1257:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	7	18	theme	mitochondrial	1003:1015	arg1	ΔΨm					1037:1039	ΔΨm	1037:1039	ΔΨm	1037:1039	Mitochondrial dysfunction in cell death is evident from the depolarization of mitochondrial membrane potential (ΔΨm ).
23852919	7	18	theme	mitochondrial	1003:1015	arg1	potential					1026:1034	mitochondrial membrane potential	1003:1034	mitochondrial membrane potential (ΔΨm )	1003:1041	Mitochondrial dysfunction in cell death is evident from the depolarization of mitochondrial membrane potential (ΔΨm ).
23852919	7	19	from	dysfunction	939:949	arg1	death					959:963	cell death	954:963	cell death	954:963	Mitochondrial dysfunction in cell death is evident from the depolarization of mitochondrial membrane potential (ΔΨm ).
23852919	9	20	theme	present	1264:1270	arg1	NPs					1260:1262	Ag NPs	1257:1262	Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs	1257:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	0	21	theme	human	92:96	arg1	cells					111:115	human glioblastoma cells	92:115	human glioblastoma cells	92:115	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier induces apoptosis in human glioblastoma cells.
23852919	9	22	theme	Ag	1275:1276	arg1	NC					1290:1291	Ag NPs-Alg-Chi NC	1275:1291	Ag NPs-Alg-Chi NC in comparison with free Ag NPs	1275:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	5	23	theme	DNA	812:814	arg1	damage					816:821	extensive DNA damage	802:821	extensive DNA damage	802:821	The cell cycle analysis shows extensive DNA damage.
23852919	1	24	theme	biodegradable	172:184	arg1	NC					279:280	Ag NPs-Alg-Chi NC	264:280	Ag NPs-Alg-Chi NC	264:280	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	1	24	theme	biodegradable	172:184	arg1	nanocarrier					251:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier	164:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC)	164:281	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	1	25	theme	green	128:132	arg1	method					134:139	a green method	126:139	a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC)	126:281	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	7	26	theme	cell	954:957	arg1	death					959:963	cell death	954:963	cell death	954:963	Mitochondrial dysfunction in cell death is evident from the depolarization of mitochondrial membrane potential (ΔΨm ).
23852919	9	27	theme	free	1312:1315	arg1	NPs					1320:1322	free Ag NPs	1312:1322	free Ag NPs	1312:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	4	28	theme	mL	753:754	arg1	IC50					738:741	IC50	738:741	IC50 of 2.4 μg mL(-1) for Ag NPs	738:769	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	3	29	theme	ultraviolet-visible	449:467	arg1	spectroscopy					469:480	ultraviolet-visible spectroscopy	449:480	ultraviolet-visible spectroscopy	449:480	The composite NC is characterized by ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction.
23852919	0	30	theme	Silver	0:5	arg1	nanocarrier					59:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier	0:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier	0:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier induces apoptosis in human glioblastoma cells.
23852919	0	31	theme	impregnated	21:31	arg1	nanocarrier					59:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier	0:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier	0:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier induces apoptosis in human glioblastoma cells.
23852919	3	32	dep	transform	561:569	arg1	infrared					571:578	infrared	571:578	transform infrared spectroscopy	561:591	The composite NC is characterized by ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction.
23852919	3	33	theme	electron	496:503	arg1	microscopy					505:514	transmission electron microscopy	483:514	transmission electron microscopy	483:514	The composite NC is characterized by ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction.
23852919	9	34	theme	promising	1335:1343	arg1	tool					1345:1348	a promising tool	1333:1348	a promising tool for cancer therapy	1333:1367	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	8	35	theme	cells	1087:1091	arg1	images					1065:1070	Fluorescence and SEM images	1044:1070	Fluorescence and SEM images of the treated cells	1044:1091	Fluorescence and SEM images of the treated cells reveal nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay.
23852919	6	36	theme	oxygen	846:851	arg1	species					853:859	reactive oxygen species	837:859	reactive oxygen species level	837:865	Elevation in reactive oxygen species level indicates induction of oxidative stress in treated cells.
23852919	2	37	theme	complex	363:369	arg1	formation					371:379	the polyelectrolyte complex formation	343:379	the polyelectrolyte complex formation between alginate and chitosan	343:409	The synthesis of Ag NPs-Alg-Chi NC is based on the polyelectrolyte complex formation between alginate and chitosan.
23852919	5	38	theme	cycle	781:785	arg1	analysis					787:794	The cell cycle analysis	772:794	The cell cycle analysis	772:794	The cell cycle analysis shows extensive DNA damage.
23852919	8	39	theme	nuclear	1100:1106	arg1	changes					1126:1132	nuclear and morphological changes	1100:1132	nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay	1100:1203	Fluorescence and SEM images of the treated cells reveal nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay.
23852919	3	40	theme	electron	526:533	arg1	SEM					547:549	SEM	547:549	SEM	547:549	The composite NC is characterized by ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction.
23852919	3	40	theme	electron	526:533	arg1	microscopy					535:544	scanning electron microscopy	517:544	scanning electron microscopy (SEM)	517:550	The composite NC is characterized by ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction.
23852919	8	41	theme	morphological	1112:1124	arg1	changes					1126:1132	nuclear and morphological changes	1100:1132	nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay	1100:1203	Fluorescence and SEM images of the treated cells reveal nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay.
23852919	1	42	theme	NPs-Alg-Chi	267:277	arg1	NC					279:280	Ag NPs-Alg-Chi NC	264:280	Ag NPs-Alg-Chi NC	264:280	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	1	42	theme	NPs-Alg-Chi	267:277	arg1	nanocarrier					251:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier	164:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC)	164:281	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	8	43	theme	characteristic	1134:1147	arg1	changes					1126:1132	nuclear and morphological changes	1100:1132	nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay	1100:1203	Fluorescence and SEM images of the treated cells reveal nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay.
23852919	8	44	theme	apoptosis	1152:1160	arg1	characteristic					1134:1147	characteristic	1134:1147	characteristic	1134:1147	Fluorescence and SEM images of the treated cells reveal nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay.
23852919	4	45	theme	Ag	621:622	arg1	NPs					624:626	The Ag NPs	617:626	The Ag NPs in the NC	617:636	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	6	46	from	induction	877:885	arg1	cells					918:922	treated cells	910:922	treated cells	910:922	Elevation in reactive oxygen species level indicates induction of oxidative stress in treated cells.
23852919	2	47	theme	Ag	313:314	arg1	NC					328:329	Ag NPs-Alg-Chi NC	313:329	Ag NPs-Alg-Chi NC	313:329	The synthesis of Ag NPs-Alg-Chi NC is based on the polyelectrolyte complex formation between alginate and chitosan.
23852919	6	48	theme	oxidative	890:898	arg1	stress					900:905	oxidative stress	890:905	oxidative stress	890:905	Elevation in reactive oxygen species level indicates induction of oxidative stress in treated cells.
23852919	1	49	theme	silver	186:191	arg1	NC					279:280	Ag NPs-Alg-Chi NC	264:280	Ag NPs-Alg-Chi NC	264:280	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	1	49	theme	silver	186:191	arg1	nanocarrier					251:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier	164:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC)	164:281	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	4	50	theme	U87MG	702:706	arg1	cells					729:733	refractory U87MG (human glioblastoma) cells	691:733	refractory U87MG (human glioblastoma) cells	691:733	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	9	51	theme	apoptosis	1223:1231	arg1	induction					1210:1218	The induction	1206:1218	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs	1206:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	2	52	theme	NC	328:329	arg1	synthesis					300:308	The synthesis	296:308	The synthesis of Ag NPs-Alg-Chi NC	296:329	The synthesis of Ag NPs-Alg-Chi NC is based on the polyelectrolyte complex formation between alginate and chitosan.
23852919	9	53	theme	low	1236:1238	arg1	concentration					1240:1252	low concentration	1236:1252	low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs	1236:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	1	54	theme	alginate-chitosan-blended	225:249	arg1	NC					279:280	Ag NPs-Alg-Chi NC	264:280	Ag NPs-Alg-Chi NC	264:280	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	1	54	theme	alginate-chitosan-blended	225:249	arg1	nanocarrier					251:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier	164:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC)	164:281	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	4	55	theme	proliferative	667:679	arg1	effect					681:686	anticell proliferative effect	658:686	anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs	658:769	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	9	56	theme	NPs	1260:1262	arg1	concentration					1240:1252	low concentration	1236:1252	low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs	1236:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	7	57	theme	membrane	1017:1024	arg1	ΔΨm					1037:1039	ΔΨm	1037:1039	ΔΨm	1037:1039	Mitochondrial dysfunction in cell death is evident from the depolarization of mitochondrial membrane potential (ΔΨm ).
23852919	7	57	theme	membrane	1017:1024	arg1	potential					1026:1034	mitochondrial membrane potential	1003:1034	mitochondrial membrane potential (ΔΨm )	1003:1041	Mitochondrial dysfunction in cell death is evident from the depolarization of mitochondrial membrane potential (ΔΨm ).
23852919	3	58	theme	X-ray	598:602	arg1	diffraction					604:614	X-ray diffraction	598:614	X-ray diffraction	598:614	The composite NC is characterized by ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction.
23852919	9	59	with	comparison	1296:1305	arg1	NPs					1320:1322	free Ag NPs	1312:1322	free Ag NPs	1312:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	0	60	theme	glioblastoma	98:109	arg1	cells					111:115	human glioblastoma cells	92:115	human glioblastoma cells	92:115	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier induces apoptosis in human glioblastoma cells.
23852919	9	61	attach	present	1264:1270	arg1	NC					1290:1291	Ag NPs-Alg-Chi NC	1275:1291	Ag NPs-Alg-Chi NC in comparison with free Ag NPs	1275:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	9	61	attach	present	1264:1270	arg2	NPs					1260:1262	Ag NPs	1257:1262	Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs	1257:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	4	62	theme	human	709:713	arg1	U87MG					702:706	refractory U87MG	691:706	refractory U87MG (human glioblastoma) cells	691:733	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	4	62	theme	human	709:713	arg1	glioblastoma					715:726	human glioblastoma	709:726	human glioblastoma	709:726	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	9	63	theme	NPs-Alg-Chi	1278:1288	arg1	NC					1290:1291	Ag NPs-Alg-Chi NC	1275:1291	Ag NPs-Alg-Chi NC in comparison with free Ag NPs	1275:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	1	64	theme	novel	166:170	arg1	NC					279:280	Ag NPs-Alg-Chi NC	264:280	Ag NPs-Alg-Chi NC	264:280	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	1	64	theme	novel	166:170	arg1	nanocarrier					251:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier	164:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC)	164:281	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	8	65	theme	Fluorescence	1044:1055	arg1	images					1065:1070	Fluorescence and SEM images	1044:1070	Fluorescence and SEM images of the treated cells	1044:1091	Fluorescence and SEM images of the treated cells reveal nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay.
23852919	4	66	from	NPs	624:626	arg1	NC					635:636	the NC	631:636	the NC	631:636	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	8	67	theme	SEM	1061:1063	arg1	images					1065:1070	Fluorescence and SEM images	1044:1070	Fluorescence and SEM images of the treated cells	1044:1091	Fluorescence and SEM images of the treated cells reveal nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay.
23852919	4	68	theme	Ag	764:765	arg1	NPs					767:769	Ag NPs	764:769	Ag NPs	764:769	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	3	69	theme	transmission	483:494	arg1	microscopy					505:514	transmission electron microscopy	483:514	transmission electron microscopy	483:514	The composite NC is characterized by ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction.
23852919	0	70	theme	nanoparticles	7:19	arg1	nanocarrier					59:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier	0:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier	0:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier induces apoptosis in human glioblastoma cells.
23852919	9	71	theme	Ag	1317:1318	arg1	NPs					1320:1322	free Ag NPs	1312:1322	free Ag NPs	1312:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	3	72	theme	composite	416:424	arg1	NC					426:427	The composite NC	412:427	The composite NC	412:427	The composite NC is characterized by ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction.
23852919	4	73	theme	μg	750:751	arg1	-1					756:757	-1	756:757	-1	756:757	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	4	73	theme	μg	750:751	arg1	mL					753:754	2.4 μg mL	746:754	2.4 μg mL(-1) for Ag NPs	746:769	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	0	74	theme	alginate-chitosan-blended	33:57	arg1	nanocarrier					59:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier	0:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier	0:69	Silver nanoparticles impregnated alginate-chitosan-blended nanocarrier induces apoptosis in human glioblastoma cells.
23852919	8	75	theme	treated	1079:1085	arg1	cells					1087:1091	the treated cells	1075:1091	the treated cells	1075:1091	Fluorescence and SEM images of the treated cells reveal nuclear and morphological changes characteristic of apoptosis, which is further confirmed by TUNEL assay.
23852919	7	76	theme	Mitochondrial	925:937	arg1	dysfunction					939:949	Mitochondrial dysfunction	925:949	Mitochondrial dysfunction in cell death	925:963	Mitochondrial dysfunction in cell death is evident from the depolarization of mitochondrial membrane potential (ΔΨm ).
23852919	3	77	dep	Fourier	553:559	arg1	transform					561:569	transform	561:569	transform infrared spectroscopy	561:591	The composite NC is characterized by ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction.
23852919	3	78	theme	scanning	517:524	arg1	SEM					547:549	SEM	547:549	SEM	547:549	The composite NC is characterized by ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction.
23852919	3	78	theme	scanning	517:524	arg1	microscopy					535:544	scanning electron microscopy	517:544	scanning electron microscopy (SEM)	517:550	The composite NC is characterized by ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction.
23852919	6	79	theme	species	853:859	arg1	level					861:865	reactive oxygen species level	837:865	reactive oxygen species level	837:865	Elevation in reactive oxygen species level indicates induction of oxidative stress in treated cells.
23852919	5	80	theme	cell	776:779	arg1	analysis					787:794	The cell cycle analysis	772:794	The cell cycle analysis	772:794	The cell cycle analysis shows extensive DNA damage.
23852919	6	81	theme	reactive	837:844	arg1	species					853:859	reactive oxygen species	837:859	reactive oxygen species level	837:865	Elevation in reactive oxygen species level indicates induction of oxidative stress in treated cells.
23852919	2	82	theme	polyelectrolyte	347:361	arg1	formation					371:379	the polyelectrolyte complex formation	343:379	the polyelectrolyte complex formation between alginate and chitosan	343:409	The synthesis of Ag NPs-Alg-Chi NC is based on the polyelectrolyte complex formation between alginate and chitosan.
23852919	9	83	theme	cancer	1354:1359	arg1	therapy					1361:1367	cancer therapy	1354:1367	cancer therapy	1354:1367	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
23852919	4	84	from	IC50	738:741	arg1	effect					681:686	anticell proliferative effect	658:686	anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs	658:769	The Ag NPs in the NC are found to elicit anticell proliferative effect on refractory U87MG (human glioblastoma) cells at IC50 of 2.4 μg mL(-1) for Ag NPs.
23852919	1	85	theme	Ag	264:265	arg1	NC					279:280	Ag NPs-Alg-Chi NC	264:280	Ag NPs-Alg-Chi NC	264:280	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	1	85	theme	Ag	264:265	arg1	nanocarrier					251:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier	164:261	a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC)	164:281	Herein, a green method for the development of a novel biodegradable silver nanoparticles (NPs) impregnated alginate-chitosan-blended nanocarrier (Ag NPs-Alg-Chi NC) is reported.
23852919	9	86	from	present	1264:1270	arg1	NC					1290:1291	Ag NPs-Alg-Chi NC	1275:1291	Ag NPs-Alg-Chi NC in comparison with free Ag NPs	1275:1322	The induction of apoptosis at low concentration of Ag NPs present in Ag NPs-Alg-Chi NC in comparison with free Ag NPs makes it a promising tool for cancer therapy.
25007822	8	0	theme	E.	1118:1119	arg1	cells					1135:1139	E. coli bacteria cells	1118:1139	E. coli bacteria cells	1118:1139	Because both BG and chitosan are antibacterial materials, the antibacterial properties of the as-produced coatings were investigated using E. coli bacteria cells.
25007822	8	1	theme	as-produced	1073:1083	arg1	coatings					1085:1092	the as-produced coatings	1069:1092	the as-produced coatings	1069:1092	Because both BG and chitosan are antibacterial materials, the antibacterial properties of the as-produced coatings were investigated using E. coli bacteria cells.
25007822	8	2	theme	coli	1121:1124	arg1	cells					1135:1139	E. coli bacteria cells	1118:1139	E. coli bacteria cells	1118:1139	Because both BG and chitosan are antibacterial materials, the antibacterial properties of the as-produced coatings were investigated using E. coli bacteria cells.
25007822	9	3	theme	coatings	1243:1250	arg1	properties					1225:1234	the antibacterial properties	1207:1234	the antibacterial properties of the coatings	1207:1250	It was shown that the BG particle size has a strong influence on the antibacterial properties of the coatings.
25007822	3	4	dep	parameter	373:381	arg1	characterization					400:415	characterization	400:415	characterization	400:415	The parameter optimization and characterization of the coatings was conducted by visual inspection and by adhesion strength tests.
25007822	3	4	dep	parameter	373:381	arg1	optimization					383:394	optimization	383:394	optimization	383:394	The parameter optimization and characterization of the coatings was conducted by visual inspection and by adhesion strength tests.
25007822	8	5	theme	bacteria	1126:1133	arg1	cells					1135:1139	E. coli bacteria cells	1118:1139	E. coli bacteria cells	1118:1139	Because both BG and chitosan are antibacterial materials, the antibacterial properties of the as-produced coatings were investigated using E. coli bacteria cells.
25007822	4	6	theme	optimized	504:512	arg1	coatings					514:521	The optimized coatings	500:521	The optimized coatings	500:521	The optimized coatings were investigated in terms of their hydroxyapatite (HA) forming ability in simulated body fluid (SBF) for up to 21 days.
25007822	6	7	theme	first	769:773	arg1	investigations					775:788	The first investigations	765:788	The first investigations	765:788	The first investigations were conducted on planar stainless steel sheets.
25007822	2	8	theme	sizes	307:311	arg1	particles					280:288	BG particles	277:288	BG particles of two different sizes	277:311	BG particles of two different sizes were used, i.e., 2 μm and 20-80 nm in average diameter.
25007822	5	9	dep	infrared	662:669	arg1	FTIR					672:675	FTIR	672:675	FTIR	672:675	Fourier transform infrared (FTIR) spectroscopy results showed the successful HA formation on the coatings after 21 days.
25007822	1	10	used	used	171:174	arg2	EPD					162:164	EPD	162:164	EPD	162:164	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	1	10	used	used	171:174	arg2	deposition					150:159	Alternating current (AC) electrophoretic deposition	109:159	Alternating current (AC) electrophoretic deposition (EPD)	109:165	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	2	11	theme	different	297:305	arg1	sizes					307:311	two different sizes	293:311	two different sizes	293:311	BG particles of two different sizes were used, i.e., 2 μm and 20-80 nm in average diameter.
25007822	3	12	theme	strength	484:491	arg1	tests					493:497	adhesion strength tests	475:497	adhesion strength tests	475:497	The parameter optimization and characterization of the coatings was conducted by visual inspection and by adhesion strength tests.
25007822	2	13	from	μm	332:333	arg1	diameter					359:366	average diameter	351:366	average diameter	351:366	BG particles of two different sizes were used, i.e., 2 μm and 20-80 nm in average diameter.
25007822	5	14	theme	successful	710:719	arg1	formation					724:732	the successful HA formation	706:732	the successful HA formation	706:732	Fourier transform infrared (FTIR) spectroscopy results showed the successful HA formation on the coatings after 21 days.
25007822	9	15	theme	BG	1164:1165	arg1	size					1176:1179	the BG particle size	1160:1179	the BG particle size	1160:1179	It was shown that the BG particle size has a strong influence on the antibacterial properties of the coatings.
25007822	2	16	from	nm	345:346	arg1	diameter					359:366	average diameter	351:366	average diameter	351:366	BG particles of two different sizes were used, i.e., 2 μm and 20-80 nm in average diameter.
25007822	1	17	theme	Alternating	109:119	arg1	EPD					162:164	EPD	162:164	EPD	162:164	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	1	17	theme	Alternating	109:119	arg1	deposition					150:159	Alternating current (AC) electrophoretic deposition	109:159	Alternating current (AC) electrophoretic deposition (EPD)	109:165	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	1	18	theme	multifunctional	187:201	arg1	coatings					213:220	multifunctional composite coatings	187:220	multifunctional composite coatings combining bioactive glass (BG) particles and chitosan	187:274	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	0	19	theme	current	12:18	arg1	deposition					36:45	current electrophoretic deposition	12:45	current electrophoretic deposition of antibacterial bioactive glass-chitosan composite coatings	12:106	Alternating current electrophoretic deposition of antibacterial bioactive glass-chitosan composite coatings.
25007822	5	20	theme	HA	721:722	arg1	formation					724:732	the successful HA formation	706:732	the successful HA formation	706:732	Fourier transform infrared (FTIR) spectroscopy results showed the successful HA formation on the coatings after 21 days.
25007822	1	21	theme	current	121:127	arg1	EPD					162:164	EPD	162:164	EPD	162:164	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	1	21	theme	current	121:127	arg1	deposition					150:159	Alternating current (AC) electrophoretic deposition	109:159	Alternating current (AC) electrophoretic deposition (EPD)	109:165	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	1	22	theme	composite	203:211	arg1	coatings					213:220	multifunctional composite coatings	187:220	multifunctional composite coatings combining bioactive glass (BG) particles and chitosan	187:274	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	6	23	theme	stainless	815:823	arg1	sheets					831:836	planar stainless steel sheets	808:836	planar stainless steel sheets	808:836	The first investigations were conducted on planar stainless steel sheets.
25007822	8	24	theme	antibacterial	1012:1024	arg1	BG					992:993	BG	992:993	BG	992:993	Because both BG and chitosan are antibacterial materials, the antibacterial properties of the as-produced coatings were investigated using E. coli bacteria cells.
25007822	8	24	theme	antibacterial	1012:1024	arg1	chitosan					999:1006	chitosan	999:1006	chitosan	999:1006	Because both BG and chitosan are antibacterial materials, the antibacterial properties of the as-produced coatings were investigated using E. coli bacteria cells.
25007822	8	24	theme	antibacterial	1012:1024	arg1	materials					1026:1034	antibacterial materials	1012:1034	antibacterial materials	1012:1034	Because both BG and chitosan are antibacterial materials, the antibacterial properties of the as-produced coatings were investigated using E. coli bacteria cells.
25007822	9	25	theme	particle	1167:1174	arg1	size					1176:1179	the BG particle size	1160:1179	the BG particle size	1160:1179	It was shown that the BG particle size has a strong influence on the antibacterial properties of the coatings.
25007822	3	26	theme	coatings	424:431	arg1	parameter					373:381	The parameter optimization and characterization	369:415	The parameter optimization and characterization of the coatings	369:431	The parameter optimization and characterization of the coatings was conducted by visual inspection and by adhesion strength tests.
25007822	5	27	dep	Fourier	644:650	arg1	transform					652:660	transform	652:660	transform infrared (FTIR) spectroscopy results	652:697	Fourier transform infrared (FTIR) spectroscopy results showed the successful HA formation on the coatings after 21 days.
25007822	1	28	theme	AC	130:131	arg1	EPD					162:164	EPD	162:164	EPD	162:164	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	1	28	theme	AC	130:131	arg1	deposition					150:159	Alternating current (AC) electrophoretic deposition	109:159	Alternating current (AC) electrophoretic deposition (EPD)	109:165	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	0	29	theme	electrophoretic	20:34	arg1	deposition					36:45	current electrophoretic deposition	12:45	current electrophoretic deposition of antibacterial bioactive glass-chitosan composite coatings	12:106	Alternating current electrophoretic deposition of antibacterial bioactive glass-chitosan composite coatings.
25007822	7	30	theme	coating	931:937	arg1	feasibility					916:926	the feasibility	912:926	the feasibility of coating of three dimensional structures by EPD	912:976	In addition, scaffolds made from a TiAl4V6 alloy were considered to show the feasibility of coating of three dimensional structures by EPD.
25007822	2	31	dep	μm	332:333	arg1	i.e.					324:327	i.e.	324:327	i.e.	324:327	BG particles of two different sizes were used, i.e., 2 μm and 20-80 nm in average diameter.
25007822	1	32	theme	bioactive	232:240	arg1	particles					253:261	bioactive glass (BG) particles	232:261	bioactive glass (BG) particles	232:261	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	0	33	theme	antibacterial	50:62	arg1	coatings					99:106	antibacterial bioactive glass-chitosan composite coatings	50:106	antibacterial bioactive glass-chitosan composite coatings	50:106	Alternating current electrophoretic deposition of antibacterial bioactive glass-chitosan composite coatings.
25007822	4	34	theme	hydroxyapatite	559:572	arg1	terms					544:548	terms	544:548	terms of their hydroxyapatite (HA) forming ability in simulated body fluid (SBF)	544:623	The optimized coatings were investigated in terms of their hydroxyapatite (HA) forming ability in simulated body fluid (SBF) for up to 21 days.
25007822	9	35	from	influence	1194:1202	arg1	properties					1225:1234	the antibacterial properties	1207:1234	the antibacterial properties of the coatings	1207:1250	It was shown that the BG particle size has a strong influence on the antibacterial properties of the coatings.
25007822	9	36	contain	has	1181:1183	arg2	influence					1194:1202	a strong influence	1185:1202	a strong influence on the antibacterial properties of the coatings	1185:1250	It was shown that the BG particle size has a strong influence on the antibacterial properties of the coatings.
25007822	9	36	contain	has	1181:1183	arg1	size					1176:1179	the BG particle size	1160:1179	the BG particle size	1160:1179	It was shown that the BG particle size has a strong influence on the antibacterial properties of the coatings.
25007822	1	37	theme	glass	242:246	arg1	particles					253:261	bioactive glass (BG) particles	232:261	bioactive glass (BG) particles	232:261	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	8	38	theme	antibacterial	1041:1053	arg1	properties					1055:1064	the antibacterial properties	1037:1064	the antibacterial properties of the as-produced coatings	1037:1092	Because both BG and chitosan are antibacterial materials, the antibacterial properties of the as-produced coatings were investigated using E. coli bacteria cells.
25007822	6	39	theme	steel	825:829	arg1	sheets					831:836	planar stainless steel sheets	808:836	planar stainless steel sheets	808:836	The first investigations were conducted on planar stainless steel sheets.
25007822	3	40	theme	visual	450:455	arg1	inspection					457:466	visual inspection	450:466	visual inspection	450:466	The parameter optimization and characterization of the coatings was conducted by visual inspection and by adhesion strength tests.
25007822	0	41	theme	glass-chitosan	74:87	arg1	coatings					99:106	antibacterial bioactive glass-chitosan composite coatings	50:106	antibacterial bioactive glass-chitosan composite coatings	50:106	Alternating current electrophoretic deposition of antibacterial bioactive glass-chitosan composite coatings.
25007822	5	42	dep	spectroscopy	678:689	arg1	results					691:697	results	691:697	results	691:697	Fourier transform infrared (FTIR) spectroscopy results showed the successful HA formation on the coatings after 21 days.
25007822	1	43	theme	electrophoretic	134:148	arg1	EPD					162:164	EPD	162:164	EPD	162:164	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	1	43	theme	electrophoretic	134:148	arg1	deposition					150:159	Alternating current (AC) electrophoretic deposition	109:159	Alternating current (AC) electrophoretic deposition (EPD)	109:165	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	0	44	theme	bioactive	64:72	arg1	coatings					99:106	antibacterial bioactive glass-chitosan composite coatings	50:106	antibacterial bioactive glass-chitosan composite coatings	50:106	Alternating current electrophoretic deposition of antibacterial bioactive glass-chitosan composite coatings.
25007822	6	45	theme	planar	808:813	arg1	sheets					831:836	planar stainless steel sheets	808:836	planar stainless steel sheets	808:836	The first investigations were conducted on planar stainless steel sheets.
25007822	7	46	theme	structures	960:969	arg1	coating					931:937	coating	931:937	coating of three dimensional structures	931:969	In addition, scaffolds made from a TiAl4V6 alloy were considered to show the feasibility of coating of three dimensional structures by EPD.
25007822	9	47	theme	strong	1187:1192	arg1	influence					1194:1202	a strong influence	1185:1202	a strong influence on the antibacterial properties of the coatings	1185:1250	It was shown that the BG particle size has a strong influence on the antibacterial properties of the coatings.
25007822	8	48	theme	coatings	1085:1092	arg1	properties					1055:1064	the antibacterial properties	1037:1064	the antibacterial properties of the as-produced coatings	1037:1092	Because both BG and chitosan are antibacterial materials, the antibacterial properties of the as-produced coatings were investigated using E. coli bacteria cells.
25007822	2	49	used	used	318:321	arg2	particles					280:288	BG particles	277:288	BG particles of two different sizes	277:311	BG particles of two different sizes were used, i.e., 2 μm and 20-80 nm in average diameter.
25007822	1	50	theme	BG	249:250	arg1	particles					253:261	bioactive glass (BG) particles	232:261	bioactive glass (BG) particles	232:261	Alternating current (AC) electrophoretic deposition (EPD) was used to produce multifunctional composite coatings combining bioactive glass (BG) particles and chitosan.
25007822	5	51	theme	infrared	662:669	arg1	spectroscopy					678:689	infrared (FTIR) spectroscopy	662:689	infrared (FTIR) spectroscopy results	662:697	Fourier transform infrared (FTIR) spectroscopy results showed the successful HA formation on the coatings after 21 days.
25007822	9	52	theme	antibacterial	1211:1223	arg1	properties					1225:1234	the antibacterial properties	1207:1234	the antibacterial properties of the coatings	1207:1250	It was shown that the BG particle size has a strong influence on the antibacterial properties of the coatings.
25007822	2	53	theme	BG	277:278	arg1	particles					280:288	BG particles	277:288	BG particles of two different sizes	277:311	BG particles of two different sizes were used, i.e., 2 μm and 20-80 nm in average diameter.
25007822	3	54	theme	adhesion	475:482	arg1	tests					493:497	adhesion strength tests	475:497	adhesion strength tests	475:497	The parameter optimization and characterization of the coatings was conducted by visual inspection and by adhesion strength tests.
25007822	0	55	theme	coatings	99:106	arg1	deposition					36:45	current electrophoretic deposition	12:45	current electrophoretic deposition of antibacterial bioactive glass-chitosan composite coatings	12:106	Alternating current electrophoretic deposition of antibacterial bioactive glass-chitosan composite coatings.
25007822	7	56	theme	dimensional	948:958	arg1	structures					960:969	three dimensional structures	942:969	three dimensional structures	942:969	In addition, scaffolds made from a TiAl4V6 alloy were considered to show the feasibility of coating of three dimensional structures by EPD.
25007822	2	57	theme	average	351:357	arg1	diameter					359:366	average diameter	351:366	average diameter	351:366	BG particles of two different sizes were used, i.e., 2 μm and 20-80 nm in average diameter.
25007822	7	58	theme	TiAl4V6	874:880	arg1	alloy					882:886	a TiAl4V6 alloy	872:886	a TiAl4V6 alloy	872:886	In addition, scaffolds made from a TiAl4V6 alloy were considered to show the feasibility of coating of three dimensional structures by EPD.
25007822	0	59	theme	composite	89:97	arg1	coatings					99:106	antibacterial bioactive glass-chitosan composite coatings	50:106	antibacterial bioactive glass-chitosan composite coatings	50:106	Alternating current electrophoretic deposition of antibacterial bioactive glass-chitosan composite coatings.
25007822	4	60	dep	21	635:636	arg1	to					632:633	to	632:633	to	632:633	The optimized coatings were investigated in terms of their hydroxyapatite (HA) forming ability in simulated body fluid (SBF) for up to 21 days.
25007822	4	61	theme	simulated	598:606	arg1	SBF					620:622	SBF	620:622	SBF	620:622	The optimized coatings were investigated in terms of their hydroxyapatite (HA) forming ability in simulated body fluid (SBF) for up to 21 days.
25007822	4	61	theme	simulated	598:606	arg1	fluid					613:617	simulated body fluid	598:617	simulated body fluid (SBF)	598:623	The optimized coatings were investigated in terms of their hydroxyapatite (HA) forming ability in simulated body fluid (SBF) for up to 21 days.
25007822	4	62	theme	body	608:611	arg1	SBF					620:622	SBF	620:622	SBF	620:622	The optimized coatings were investigated in terms of their hydroxyapatite (HA) forming ability in simulated body fluid (SBF) for up to 21 days.
25007822	4	62	theme	body	608:611	arg1	fluid					613:617	simulated body fluid	598:617	simulated body fluid (SBF)	598:623	The optimized coatings were investigated in terms of their hydroxyapatite (HA) forming ability in simulated body fluid (SBF) for up to 21 days.
26959641	2	0	theme	developing	516:525	arg1	limb					527:530	the developing limb	512:530	the developing limb	512:530	Therefore, a biosynthetic hydrogel was developed containing RGD, hyaluronic acid and/or type-I collagen conjugated to poly(ethylene glycol) acrylate to recapitulate the precondensation microenvironment of the developing limb.
26959641	0	1	from	Effects	16:22	arg1	Response					67:74	the Response	63:74	the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment	63:142	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	2	2	theme	hyaluronic	372:381	arg1	acid					383:386	hyaluronic acid	372:386	hyaluronic acid	372:386	Therefore, a biosynthetic hydrogel was developed containing RGD, hyaluronic acid and/or type-I collagen conjugated to poly(ethylene glycol) acrylate to recapitulate the precondensation microenvironment of the developing limb.
26959641	1	3	theme	functional	258:267	arg1	evolution					269:277	the functional evolution	254:277	the functional evolution of native cartilage tissue	254:304	Improve functional quality of cartilage tissue engineered from stem cells requires a better understanding of the functional evolution of native cartilage tissue.
26959641	4	4	theme	sGAG	982:985	arg1	production					987:996	sGAG production	982:996	sGAG production	982:996	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	3	5	theme	composition	692:702	arg1	influence					672:680	the influence	668:680	the influence of ligand composition on chondrogenesis	668:720	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	4	6	theme	markers	862:868	arg1	chondrogenesis					939:952	chondrogenesis	939:952	chondrogenesis (Col2a1 gene expression and sGAG production)	939:997	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	6	theme	markers	862:868	arg1	expression					793:802	Gene expression	788:802	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression)	788:933	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	7	from	expression	793:802	arg1	system					1034:1039	this system	1029:1039	this system	1029:1039	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	8	theme	matrix	1064:1069	arg1	stiffness					1071:1079	matrix stiffness	1064:1079	matrix stiffness	1064:1079	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	1	9	theme	evolution	269:277	arg1	understanding					237:249	a better understanding	228:249	a better understanding of the functional evolution of native cartilage tissue	228:304	Improve functional quality of cartilage tissue engineered from stem cells requires a better understanding of the functional evolution of native cartilage tissue.
26959641	0	10	theme	Cells	97:101	arg1	Response					67:74	the Response	63:74	the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment	63:142	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	6	11	theme	physicochemical	1509:1523	arg1	properties					1525:1534	the matrix physicochemical properties	1498:1534	the matrix physicochemical properties	1498:1534	This biosynthetic hydrogel supports the features of early limb-bud condensation and chondrogenesis and is a novel platform in which the influence of the matrix physicochemical properties on these processes can be elucidated.
26959641	3	12	from	influence	672:680	arg1	chondrogenesis					707:720	chondrogenesis	707:720	chondrogenesis	707:720	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	0	13	theme	Chondroprogenitor	79:95	arg1	Cells					97:101	Chondroprogenitor Cells	79:101	Chondroprogenitor Cells in a 3D Precondensation Microenvironment	79:142	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	4	14	theme	condensation	873:884	arg1	receptors					814:822	ligand receptors	807:822	ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression)	807:933	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	14	theme	condensation	873:884	arg1	CD44					825:828	CD44	825:828	CD44	825:828	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	14	theme	condensation	873:884	arg1	markers					862:868	markers	862:868	ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression)	807:933	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	14	theme	condensation	873:884	arg1	b1-integrin					838:848	the b1-integrin	834:848	the b1-integrin	834:848	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	3	15	theme	ligands	577:583	arg1	combination					552:562	any combination	548:562	any combination of the three ligands	548:583	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	5	16	theme	stiffness-dependent	1321:1339	arg1	manner					1341:1346	a stiffness-dependent manner	1319:1346	a stiffness-dependent manner	1319:1346	Cell proliferation in these 3D matrices for 7 d prior to chondrogenic induction increased the rate of sGAG production in a stiffness-dependent manner.
26959641	1	17	theme	native	282:287	arg1	tissue					299:304	native cartilage tissue	282:304	native cartilage tissue	282:304	Improve functional quality of cartilage tissue engineered from stem cells requires a better understanding of the functional evolution of native cartilage tissue.
26959641	4	18	theme	gene	962:965	arg1	expression					967:976	Col2a1 gene expression	955:976	Col2a1 gene expression	955:976	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	5	19	theme	3D	1226:1227	arg1	matrices					1229:1236	these 3D matrices	1220:1236	these 3D matrices	1220:1236	Cell proliferation in these 3D matrices for 7 d prior to chondrogenic induction increased the rate of sGAG production in a stiffness-dependent manner.
26959641	4	20	theme	gene	1122:1125	arg1	expression					1127:1136	the highest gene expression	1110:1136	the highest gene expression occurring in softer hydrogels containing all three ligands	1110:1195	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	1	21	theme	cartilage	175:183	arg1	tissue					185:190	cartilage tissue	175:190	cartilage tissue engineered from stem cells	175:217	Improve functional quality of cartilage tissue engineered from stem cells requires a better understanding of the functional evolution of native cartilage tissue.
26959641	4	22	theme	Col2a1	955:960	arg1	expression					967:976	Col2a1 gene expression	955:976	Col2a1 gene expression	955:976	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	6	23	theme	biosynthetic	1354:1365	arg1	hydrogel					1367:1374	This biosynthetic hydrogel	1349:1374	This biosynthetic hydrogel	1349:1374	This biosynthetic hydrogel supports the features of early limb-bud condensation and chondrogenesis and is a novel platform in which the influence of the matrix physicochemical properties on these processes can be elucidated.
26959641	4	24	dep	receptors	814:822	arg1	receptors					814:822	ligand receptors	807:822	ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression)	807:933	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	24	dep	receptors	814:822	arg1	CD44					825:828	CD44	825:828	CD44	825:828	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	24	dep	receptors	814:822	arg1	b1-integrin					838:848	the b1-integrin	834:848	the b1-integrin	834:848	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	25	theme	Gene	788:791	arg1	expression					793:802	Gene expression	788:802	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression)	788:933	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	1	26	theme	tissue	185:190	arg1	quality					164:170	functional quality	153:170	functional quality of cartilage tissue engineered from stem cells	153:217	Improve functional quality of cartilage tissue engineered from stem cells requires a better understanding of the functional evolution of native cartilage tissue.
26959641	0	27	theme	Synergistic	4:14	arg1	Effects					16:22	The Synergistic Effects	0:22	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment	0:142	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	3	28	theme	varying	762:768	arg1	stiffness					777:785	varying matrix stiffness	762:785	varying matrix stiffness	762:785	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	4	29	theme	highest	1114:1120	arg1	expression					1127:1136	the highest gene expression	1110:1136	the highest gene expression occurring in softer hydrogels containing all three ligands	1110:1195	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	1	30	theme	cartilage	289:297	arg1	tissue					299:304	native cartilage tissue	282:304	native cartilage tissue	282:304	Improve functional quality of cartilage tissue engineered from stem cells requires a better understanding of the functional evolution of native cartilage tissue.
26959641	2	31	theme	biosynthetic	320:331	arg1	hydrogel					333:340	a biosynthetic hydrogel	318:340	a biosynthetic hydrogel	318:340	Therefore, a biosynthetic hydrogel was developed containing RGD, hyaluronic acid and/or type-I collagen conjugated to poly(ethylene glycol) acrylate to recapitulate the precondensation microenvironment of the developing limb.
26959641	5	32	theme	sGAG	1300:1303	arg1	production					1305:1314	sGAG production	1300:1314	sGAG production	1300:1314	Cell proliferation in these 3D matrices for 7 d prior to chondrogenic induction increased the rate of sGAG production in a stiffness-dependent manner.
26959641	2	33	theme	type-I	395:400	arg1	collagen					402:409	type-I collagen	395:409	type-I collagen	395:409	Therefore, a biosynthetic hydrogel was developed containing RGD, hyaluronic acid and/or type-I collagen conjugated to poly(ethylene glycol) acrylate to recapitulate the precondensation microenvironment of the developing limb.
26959641	1	34	theme	tissue	299:304	arg1	evolution					269:277	the functional evolution	254:277	the functional evolution of native cartilage tissue	254:304	Improve functional quality of cartilage tissue engineered from stem cells requires a better understanding of the functional evolution of native cartilage tissue.
26959641	0	35	theme	Precondensation	111:125	arg1	Microenvironment					127:142	a 3D Precondensation Microenvironment	106:142	a 3D Precondensation Microenvironment	106:142	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	5	36	theme	production	1305:1314	arg1	rate					1292:1295	the rate	1288:1295	the rate of sGAG production	1288:1314	Cell proliferation in these 3D matrices for 7 d prior to chondrogenic induction increased the rate of sGAG production in a stiffness-dependent manner.
26959641	1	37	theme	stem	208:211	arg1	cells					213:217	stem cells	208:217	stem cells	208:217	Improve functional quality of cartilage tissue engineered from stem cells requires a better understanding of the functional evolution of native cartilage tissue.
26959641	0	38	theme	3D	108:109	arg1	Microenvironment					127:142	a 3D Precondensation Microenvironment	106:142	a 3D Precondensation Microenvironment	106:142	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	6	39	theme	chondrogenesis	1433:1446	arg1	features					1389:1396	the features	1385:1396	the features of early limb-bud condensation and chondrogenesis	1385:1446	This biosynthetic hydrogel supports the features of early limb-bud condensation and chondrogenesis and is a novel platform in which the influence of the matrix physicochemical properties on these processes can be elucidated.
26959641	6	40	theme	properties	1525:1534	arg1	influence					1485:1493	the influence	1481:1493	the influence of the matrix physicochemical properties on these processes	1481:1553	This biosynthetic hydrogel supports the features of early limb-bud condensation and chondrogenesis and is a novel platform in which the influence of the matrix physicochemical properties on these processes can be elucidated.
26959641	2	41	theme	precondensation	476:490	arg1	microenvironment					492:507	the precondensation microenvironment	472:507	the precondensation microenvironment of the developing limb	472:530	Therefore, a biosynthetic hydrogel was developed containing RGD, hyaluronic acid and/or type-I collagen conjugated to poly(ethylene glycol) acrylate to recapitulate the precondensation microenvironment of the developing limb.
26959641	2	42	theme	ethylene	430:437	arg1	poly					425:428	poly	425:428	poly(ethylene glycol) acrylate	425:454	Therefore, a biosynthetic hydrogel was developed containing RGD, hyaluronic acid and/or type-I collagen conjugated to poly(ethylene glycol) acrylate to recapitulate the precondensation microenvironment of the developing limb.
26959641	2	42	theme	ethylene	430:437	arg1	glycol					439:444	ethylene glycol	430:444	ethylene glycol	430:444	Therefore, a biosynthetic hydrogel was developed containing RGD, hyaluronic acid and/or type-I collagen conjugated to poly(ethylene glycol) acrylate to recapitulate the precondensation microenvironment of the developing limb.
26959641	0	43	theme	Stiffness	34:42	arg1	Effects					16:22	The Synergistic Effects	0:22	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment	0:142	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	5	44	theme	Cell	1198:1201	arg1	proliferation					1203:1215	Cell proliferation	1198:1215	Cell proliferation in these 3D matrices for 7 d prior to chondrogenic induction	1198:1276	Cell proliferation in these 3D matrices for 7 d prior to chondrogenic induction increased the rate of sGAG production in a stiffness-dependent manner.
26959641	5	45	from	proliferation	1203:1215	arg1	matrices					1229:1236	these 3D matrices	1220:1236	these 3D matrices	1220:1236	Cell proliferation in these 3D matrices for 7 d prior to chondrogenic induction increased the rate of sGAG production in a stiffness-dependent manner.
26959641	0	46	from	Response	67:74	arg1	Microenvironment					127:142	a 3D Precondensation Microenvironment	106:142	a 3D Precondensation Microenvironment	106:142	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	4	47	theme	ligand	1085:1090	arg1	composition					1092:1102	ligand composition	1085:1102	ligand composition	1085:1102	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	5	48	theme	chondrogenic	1255:1266	arg1	induction					1268:1276	chondrogenic induction	1255:1276	chondrogenic induction	1255:1276	Cell proliferation in these 3D matrices for 7 d prior to chondrogenic induction increased the rate of sGAG production in a stiffness-dependent manner.
26959641	0	49	theme	Matrix	27:32	arg1	Stiffness					34:42	Matrix Stiffness	27:42	Matrix Stiffness	27:42	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	6	50	theme	early	1401:1405	arg1	condensation					1416:1427	early limb-bud condensation	1401:1427	early limb-bud condensation	1401:1427	This biosynthetic hydrogel supports the features of early limb-bud condensation and chondrogenesis and is a novel platform in which the influence of the matrix physicochemical properties on these processes can be elucidated.
26959641	4	51	dep	chondrogenesis	939:952	arg1	expression					967:976	Col2a1 gene expression	955:976	Col2a1 gene expression	955:976	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	51	dep	chondrogenesis	939:952	arg1	production					987:996	sGAG production	982:996	sGAG production	982:996	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	3	52	theme	hydrogels	651:659	arg1	moduli					609:614	shear moduli	603:614	shear moduli	603:614	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	3	52	theme	hydrogels	651:659	arg1	properties					629:638	diffusion properties	619:638	diffusion properties	619:638	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	3	53	theme	diffusion	619:627	arg1	properties					629:638	diffusion properties	619:638	diffusion properties	619:638	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	2	54	theme	poly	425:428	arg1	acrylate					447:454	poly(ethylene glycol) acrylate	425:454	poly(ethylene glycol) acrylate	425:454	Therefore, a biosynthetic hydrogel was developed containing RGD, hyaluronic acid and/or type-I collagen conjugated to poly(ethylene glycol) acrylate to recapitulate the precondensation microenvironment of the developing limb.
26959641	0	55	theme	Composition	48:58	arg1	Effects					16:22	The Synergistic Effects	0:22	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment	0:142	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	3	56	theme	matrix	770:775	arg1	stiffness					777:785	varying matrix stiffness	762:785	varying matrix stiffness	762:785	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	3	57	theme	shear	603:607	arg1	moduli					609:614	shear moduli	603:614	shear moduli	603:614	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	6	58	theme	novel	1457:1461	arg1	platform					1463:1470	a novel platform	1455:1470	a novel platform in which the influence of the matrix physicochemical properties on these processes can be elucidated	1455:1571	This biosynthetic hydrogel supports the features of early limb-bud condensation and chondrogenesis and is a novel platform in which the influence of the matrix physicochemical properties on these processes can be elucidated.
26959641	4	59	theme	chondroprogenitor	1002:1018	arg1	cells					1020:1024	chondroprogenitor cells	1002:1024	chondroprogenitor cells	1002:1024	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	3	60	theme	stiffness	777:785	arg1	context					751:757	the context	747:757	the context of varying matrix stiffness	747:785	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	4	61	theme	cell	887:890	arg1	clustering					892:901	cell clustering	887:901	cell clustering	887:901	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	62	from	chondrogenesis	939:952	arg1	system					1034:1039	this system	1029:1039	this system	1029:1039	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	63	dep	condensation	873:884	arg1	expression					923:932	N-cadherin gene expression	907:932	N-cadherin gene expression	907:932	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	63	dep	condensation	873:884	arg1	clustering					892:901	cell clustering	887:901	cell clustering	887:901	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	64	theme	N-cadherin	907:916	arg1	expression					923:932	N-cadherin gene expression	907:932	N-cadherin gene expression	907:932	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	3	65	theme	ligand	685:690	arg1	composition					692:702	ligand composition	685:702	ligand composition	685:702	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	4	66	theme	ligand	807:812	arg1	receptors					814:822	ligand receptors	807:822	ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression)	807:933	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	66	theme	ligand	807:812	arg1	CD44					825:828	CD44	825:828	CD44	825:828	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	66	theme	ligand	807:812	arg1	b1-integrin					838:848	the b1-integrin	834:848	the b1-integrin	834:848	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	6	67	theme	limb-bud	1407:1414	arg1	condensation					1416:1427	early limb-bud condensation	1401:1427	early limb-bud condensation	1401:1427	This biosynthetic hydrogel supports the features of early limb-bud condensation and chondrogenesis and is a novel platform in which the influence of the matrix physicochemical properties on these processes can be elucidated.
26959641	6	68	theme	matrix	1502:1507	arg1	properties					1525:1534	the matrix physicochemical properties	1498:1534	the matrix physicochemical properties	1498:1534	This biosynthetic hydrogel supports the features of early limb-bud condensation and chondrogenesis and is a novel platform in which the influence of the matrix physicochemical properties on these processes can be elucidated.
26959641	1	69	theme	better	230:235	arg1	understanding					237:249	a better understanding	228:249	a better understanding of the functional evolution of native cartilage tissue	228:304	Improve functional quality of cartilage tissue engineered from stem cells requires a better understanding of the functional evolution of native cartilage tissue.
26959641	0	70	from	Cells	97:101	arg1	Microenvironment					127:142	a 3D Precondensation Microenvironment	106:142	a 3D Precondensation Microenvironment	106:142	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	3	71	theme	PEG	647:649	arg1	hydrogels					651:659	the PEG hydrogels	643:659	the PEG hydrogels	643:659	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	3	72	theme	combination	552:562	arg1	Conjugation					533:543	Conjugation	533:543	Conjugation of any combination of the three ligands	533:583	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	4	73	theme	gene	918:921	arg1	expression					923:932	N-cadherin gene expression	907:932	N-cadherin gene expression	907:932	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	0	74	from	Microenvironment	127:142	arg1	Response					67:74	the Response	63:74	the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment	63:142	The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment.
26959641	4	75	contain	containing	1168:1177	arg2	ligands					1189:1195	all three ligands	1179:1195	all three ligands	1179:1195	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	75	contain	containing	1168:1177	arg1	hydrogels					1158:1166	softer hydrogels	1151:1166	softer hydrogels containing all three ligands	1151:1195	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	6	76	from	influence	1485:1493	arg1	processes					1545:1553	these processes	1539:1553	these processes	1539:1553	This biosynthetic hydrogel supports the features of early limb-bud condensation and chondrogenesis and is a novel platform in which the influence of the matrix physicochemical properties on these processes can be elucidated.
26959641	4	77	theme	softer	1151:1156	arg1	hydrogels					1158:1166	softer hydrogels	1151:1166	softer hydrogels containing all three ligands	1151:1195	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	6	78	theme	condensation	1416:1427	arg1	features					1389:1396	the features	1385:1396	the features of early limb-bud condensation and chondrogenesis	1385:1446	This biosynthetic hydrogel supports the features of early limb-bud condensation and chondrogenesis and is a novel platform in which the influence of the matrix physicochemical properties on these processes can be elucidated.
26959641	3	79	dep	moduli	609:614	arg1	the					599:601	the	599:601	the	599:601	Conjugation of any combination of the three ligands did not alter the shear moduli or diffusion properties of the PEG hydrogels; thus, the influence of ligand composition on chondrogenesis could be investigated in the context of varying matrix stiffness.
26959641	2	80	theme	limb	527:530	arg1	microenvironment					492:507	the precondensation microenvironment	472:507	the precondensation microenvironment of the developing limb	472:530	Therefore, a biosynthetic hydrogel was developed containing RGD, hyaluronic acid and/or type-I collagen conjugated to poly(ethylene glycol) acrylate to recapitulate the precondensation microenvironment of the developing limb.
26959641	4	81	theme	receptors	814:822	arg1	chondrogenesis					939:952	chondrogenesis	939:952	chondrogenesis (Col2a1 gene expression and sGAG production)	939:997	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	4	81	theme	receptors	814:822	arg1	expression					793:802	Gene expression	788:802	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression)	788:933	Gene expression of ligand receptors (CD44 and the b1-integrin) as well as markers of condensation (cell clustering and N-cadherin gene expression) and chondrogenesis (Col2a1 gene expression and sGAG production) by chondroprogenitor cells in this system were modulated by both matrix stiffness and ligand composition, with the highest gene expression occurring in softer hydrogels containing all three ligands.
26959641	1	82	theme	functional	153:162	arg1	quality					164:170	functional quality	153:170	functional quality of cartilage tissue engineered from stem cells	153:217	Improve functional quality of cartilage tissue engineered from stem cells requires a better understanding of the functional evolution of native cartilage tissue.
25362893	4	0	theme	CO2	730:732	arg1	environments					734:745	all four p CO2 environments	719:745	all four p CO2 environments	719:745	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	6	1	theme	whole	945:949	arg1	composition					967:977	whole body fatty acid composition	945:977	whole body fatty acid composition	945:977	Elevated p CO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock.
25362893	8	2	theme	identifier	1506:1515	arg1	PXD000835					1517:1525	the identifier PXD000835	1502:1525	the identifier PXD000835	1502:1525	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	2	3	theme	pH	378:379	arg1	reductions					381:390	projected end-of-century open ocean pH reductions	342:390	projected end-of-century open ocean pH reductions	342:390	The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions.
25362893	10	4	from	response	1745:1752	arg1	assessment					1693:1702	the assessment	1689:1702	the assessment of the effects of p CO2 on the proteomic response to a second stress	1689:1771	This is especially apparent in the assessment of the effects of p CO2 on the proteomic response to a second stress.
25362893	9	5	theme	Oyster	1541:1546	arg1	physiology					1548:1557	CONCLUSIONS Oyster physiology	1529:1557	CONCLUSIONS Oyster physiology	1529:1557	CONCLUSIONS Oyster physiology is significantly altered by exposure to elevated p CO2, indicating changes in energy resource use.
25362893	7	6	theme	several	1088:1094	arg1	pathways					1110:1117	several physiological pathways	1088:1117	several physiological pathways	1088:1117	Shotgun proteomics revealed that several physiological pathways were significantly affected by ocean acidification, including antioxidant response, carbohydrate metabolism, and transcription and translation.
25362893	3	7	theme	p	545:545	arg1	levels					551:556	one of four different p CO2 levels	523:556	one of four different p CO2 levels	523:556	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	9	8	theme	CONCLUSIONS	1529:1539	arg1	physiology					1548:1557	CONCLUSIONS Oyster physiology	1529:1557	CONCLUSIONS Oyster physiology	1529:1557	CONCLUSIONS Oyster physiology is significantly altered by exposure to elevated p CO2, indicating changes in energy resource use.
25362893	8	9	from	ProteomeXchange	1480:1494	arg1	available					1463:1471	available	1463:1471	available	1463:1471	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	9	10	theme	elevated	1599:1606	arg1	CO2					1610:1612	elevated p CO2	1599:1612	elevated p CO2	1599:1612	CONCLUSIONS Oyster physiology is significantly altered by exposure to elevated p CO2, indicating changes in energy resource use.
25362893	12	11	theme	acidification	1978:1990	arg1	effects					1961:1967	the effects	1957:1967	the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress	1957:2065	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	2	12	theme	ocean	252:256	arg1	experiences					258:268	The coastal ocean experiences	240:268	The coastal ocean experiences	240:268	The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions.
25362893	5	13	theme	elevated	897:904	arg1	CO2					908:910	elevated p CO2	897:910	elevated p CO2	897:910	However, micromechanical properties of the new shell were compromised by elevated p CO2.
25362893	11	14	theme	ocean	1819:1823	arg1	acidification					1825:1837	ocean acidification	1819:1837	ocean acidification	1819:1837	The altered stress response illustrates that ocean acidification may impact how oysters respond to other changes in their environment.
25362893	12	15	from	view	1949:1952	arg1	trade-offs					2028:2037	physiological trade-offs	2014:2037	oysters as well as physiological trade-offs during environmental stress	1995:2065	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	12	15	from	view	1949:1952	arg1	oysters					1995:2001	oysters	1995:2001	oysters as well as physiological trade-offs during environmental stress	1995:2065	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	8	16	dep	CO2	1434:1436	arg1	available					1463:1471	available	1463:1471	available	1463:1471	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	10	17	theme	effects	1711:1717	arg1	assessment					1693:1702	the assessment	1689:1702	the assessment of the effects of p CO2 on the proteomic response to a second stress	1689:1771	This is especially apparent in the assessment of the effects of p CO2 on the proteomic response to a second stress.
25362893	1	18	theme	increased	141:149	arg1	emissions					169:177	increased anthropogenic CO2 emissions	141:177	increased anthropogenic CO2 emissions	141:177	BACKGROUND Ocean acidification as a result of increased anthropogenic CO2 emissions is occurring in marine and estuarine environments worldwide.
25362893	9	19	from	changes	1626:1632	arg1	use					1653:1655	energy resource use	1637:1655	energy resource use	1637:1655	CONCLUSIONS Oyster physiology is significantly altered by exposure to elevated p CO2, indicating changes in energy resource use.
25362893	6	20	theme	heat	1043:1046	arg1	shock					1048:1052	acute heat shock	1037:1052	acute heat shock	1037:1052	Elevated p CO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock.
25362893	3	21	from	impact	416:421	arg1	invertebrates					456:468	marine invertebrates	449:468	marine invertebrates	449:468	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	1	22	theme	CO2	165:167	arg1	emissions					169:177	increased anthropogenic CO2 emissions	141:177	increased anthropogenic CO2 emissions	141:177	BACKGROUND Ocean acidification as a result of increased anthropogenic CO2 emissions is occurring in marine and estuarine environments worldwide.
25362893	9	23	theme	resource	1644:1651	arg1	use					1653:1655	energy resource use	1637:1655	energy resource use	1637:1655	CONCLUSIONS Oyster physiology is significantly altered by exposure to elevated p CO2, indicating changes in energy resource use.
25362893	8	24	theme	mechanical	1390:1399	arg1	stimulation					1401:1411	mechanical stimulation	1390:1411	mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835)	1390:1526	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	3	25	dep	assess	405:410	arg1	to					402:403	to	402:403	to	402:403	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	12	26	from	oysters	1995:2001	arg1	view					1949:1952	an integrative view	1934:1952	an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress	1934:2065	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	5	27	theme	micromechanical	833:847	arg1	properties					849:858	micromechanical properties	833:858	micromechanical properties of the new shell	833:875	However, micromechanical properties of the new shell were compromised by elevated p CO2.
25362893	10	28	from	assessment	1693:1702	arg1	response					1745:1752	the proteomic response	1731:1752	the proteomic response to a second stress	1731:1771	This is especially apparent in the assessment of the effects of p CO2 on the proteomic response to a second stress.
25362893	10	28	from	assessment	1693:1702	arg1	apparent					1677:1684	apparent	1677:1684	apparent	1677:1684	This is especially apparent in the assessment of the effects of p CO2 on the proteomic response to a second stress.
25362893	0	29	theme	Shotgun	0:6	arg1	proteomics					8:17	Shotgun proteomics	0:17	Shotgun proteomics	0:17	Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas.
25362893	1	30	theme	Ocean	106:110	arg1	acidification					112:124	BACKGROUND Ocean acidification	95:124	BACKGROUND Ocean acidification as a result of increased anthropogenic CO2 emissions	95:177	BACKGROUND Ocean acidification as a result of increased anthropogenic CO2 emissions is occurring in marine and estuarine environments worldwide.
25362893	6	31	theme	Elevated	913:920	arg1	CO2					924:926	Elevated p CO2	913:926	Elevated p CO2	913:926	Elevated p CO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock.
25362893	8	32	theme	p	1432:1432	arg1	CO2					1434:1436	high versus low p CO2	1416:1436	high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835)	1416:1526	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	4	33	theme	exposure	691:698	arg1	oysters					708:714	oysters	708:714	oysters in all four p CO2 environments	708:745	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	4	33	theme	exposure	691:698	arg1	period					700:705	the four week exposure period	677:705	the four week exposure period	677:705	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	7	34	theme	antioxidant	1181:1191	arg1	response					1193:1200	antioxidant response	1181:1200	antioxidant response	1181:1200	Shotgun proteomics revealed that several physiological pathways were significantly affected by ocean acidification, including antioxidant response, carbohydrate metabolism, and transcription and translation.
25362893	3	35	theme	ocean	426:430	arg1	acidification					432:444	ocean acidification	426:444	ocean acidification	426:444	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	11	36	theme	altered	1778:1784	arg1	response					1793:1800	The altered stress response	1774:1800	The altered stress response	1774:1800	The altered stress response illustrates that ocean acidification may impact how oysters respond to other changes in their environment.
25362893	8	37	theme	proteomic	1281:1289	arg1	response					1291:1298	the proteomic response	1277:1298	the proteomic response to a second stress	1277:1317	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	3	38	theme	Pacific	471:477	arg1	oysters					479:485	Pacific oysters	471:485	Pacific oysters (Crassostrea gigas)	471:505	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	3	38	theme	Pacific	471:477	arg1	gigas					500:504	Crassostrea gigas	488:504	Crassostrea gigas	488:504	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	12	39	theme	physiological	2014:2026	arg1	trade-offs					2028:2037	physiological trade-offs	2014:2037	oysters as well as physiological trade-offs during environmental stress	1995:2065	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	8	40	theme	proteomics	1443:1452	arg1	data					1454:1457	all proteomics data	1439:1457	all proteomics data	1439:1457	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	10	41	theme	p	1722:1722	arg1	CO2					1724:1726	p CO2	1722:1726	p CO2	1722:1726	This is especially apparent in the assessment of the effects of p CO2 on the proteomic response to a second stress.
25362893	2	42	theme	ocean	372:376	arg1	reductions					381:390	projected end-of-century open ocean pH reductions	342:390	projected end-of-century open ocean pH reductions	342:390	The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions.
25362893	0	43	theme	ocean	53:57	arg1	acidification					59:71	ocean acidification	53:71	ocean acidification in Crassostrea gigas	53:92	Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas.
25362893	7	44	theme	ocean	1150:1154	arg1	acidification					1156:1168	ocean acidification	1150:1168	ocean acidification	1150:1168	Shotgun proteomics revealed that several physiological pathways were significantly affected by ocean acidification, including antioxidant response, carbohydrate metabolism, and transcription and translation.
25362893	8	45	theme	second	1305:1310	arg1	stress					1312:1317	a second stress	1303:1317	a second stress	1303:1317	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	12	46	theme	environmental	2046:2058	arg1	stress					2060:2065	environmental stress	2046:2065	environmental stress	2046:2065	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	2	47	theme	end-of-century	352:365	arg1	reductions					381:390	projected end-of-century open ocean pH reductions	342:390	projected end-of-century open ocean pH reductions	342:390	The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions.
25362893	11	48	theme	other	1873:1877	arg1	changes					1879:1885	other changes	1873:1885	other changes in their environment	1873:1906	The altered stress response illustrates that ocean acidification may impact how oysters respond to other changes in their environment.
25362893	6	49	theme	acid	962:965	arg1	composition					967:977	whole body fatty acid composition	945:977	whole body fatty acid composition	945:977	Elevated p CO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock.
25362893	10	50	theme	proteomic	1735:1743	arg1	response					1745:1752	the proteomic response	1731:1752	the proteomic response to a second stress	1731:1771	This is especially apparent in the assessment of the effects of p CO2 on the proteomic response to a second stress.
25362893	4	51	theme	four	681:684	arg1	week					686:689	week	686:689	week	686:689	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	5	52	theme	new	867:869	arg1	shell					871:875	the new shell	863:875	the new shell	863:875	However, micromechanical properties of the new shell were compromised by elevated p CO2.
25362893	6	53	theme	body	951:954	arg1	composition					967:977	whole body fatty acid composition	945:977	whole body fatty acid composition	945:977	Elevated p CO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock.
25362893	12	54	from	trade-offs	2028:2037	arg1	view					1949:1952	an integrative view	1934:1952	an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress	1934:2065	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	4	55	theme	p	728:728	arg1	environments					734:745	all four p CO2 environments	719:745	all four p CO2 environments	719:745	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	7	56	theme	physiological	1096:1108	arg1	pathways					1110:1117	several physiological pathways	1088:1117	several physiological pathways	1088:1117	Shotgun proteomics revealed that several physiological pathways were significantly affected by ocean acidification, including antioxidant response, carbohydrate metabolism, and transcription and translation.
25362893	11	57	from	changes	1879:1885	arg1	environment					1896:1906	their environment	1890:1906	their environment	1890:1906	The altered stress response illustrates that ocean acidification may impact how oysters respond to other changes in their environment.
25362893	10	58	theme	second	1759:1764	arg1	stress					1766:1771	a second stress	1757:1771	a second stress	1757:1771	This is especially apparent in the assessment of the effects of p CO2 on the proteomic response to a second stress.
25362893	8	59	from	CO2	1434:1436	arg1	stimulation					1401:1411	mechanical stimulation	1390:1411	mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835)	1390:1526	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	3	60	theme	CO2	547:549	arg1	levels					551:556	one of four different p CO2 levels	523:556	one of four different p CO2 levels	523:556	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	8	61	from	available	1463:1471	arg1	ProteomeXchange					1480:1494	the ProteomeXchange	1476:1494	the ProteomeXchange	1476:1494	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	3	62	theme	different	535:543	arg1	levels					551:556	one of four different p CO2 levels	523:556	one of four different p CO2 levels	523:556	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	6	63	theme	mortality	1006:1014	arg1	rate					1016:1019	mortality rate	1006:1019	mortality rate	1006:1019	Elevated p CO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock.
25362893	1	64	theme	marine	195:200	arg1	environments					216:227	marine and estuarine environments	195:227	environments	216:227	BACKGROUND Ocean acidification as a result of increased anthropogenic CO2 emissions is occurring in marine and estuarine environments worldwide.
25362893	1	65	theme	estuarine	206:214	arg1	environments					216:227	marine and estuarine environments	195:227	environments	216:227	BACKGROUND Ocean acidification as a result of increased anthropogenic CO2 emissions is occurring in marine and estuarine environments worldwide.
25362893	4	66	theme	growth	772:777	arg1	rate					779:782	growth rate	772:782	growth rate	772:782	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	8	67	theme	p	1333:1333	arg1	CO2					1335:1337	p CO2	1333:1337	p CO2	1333:1337	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	6	68	theme	glycogen	984:991	arg1	content					993:999	glycogen content	984:999	glycogen content	984:999	Elevated p CO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock.
25362893	12	69	theme	effects	1961:1967	arg1	view					1949:1952	an integrative view	1934:1952	an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress	1934:2065	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	9	70	theme	p	1608:1608	arg1	CO2					1610:1612	elevated p CO2	1599:1612	elevated p CO2	1599:1612	CONCLUSIONS Oyster physiology is significantly altered by exposure to elevated p CO2, indicating changes in energy resource use.
25362893	12	71	theme	ocean	1972:1976	arg1	acidification					1978:1990	ocean acidification	1972:1990	ocean acidification	1972:1990	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	2	72	theme	coastal	244:250	arg1	experiences					258:268	The coastal ocean experiences	240:268	The coastal ocean experiences	240:268	The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions.
25362893	4	73	theme	new	757:759	arg1	shell					761:765	new shell	757:765	new shell	757:765	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	8	74	theme	numerous	1345:1352	arg1	processes					1354:1362	numerous processes	1345:1362	numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835)	1345:1526	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	12	75	theme	integrative	1937:1947	arg1	view					1949:1952	an integrative view	1934:1952	an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress	1934:2065	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	5	76	theme	p	906:906	arg1	CO2					908:910	elevated p CO2	897:910	elevated p CO2	897:910	However, micromechanical properties of the new shell were compromised by elevated p CO2.
25362893	2	77	theme	seasonal	291:298	arg1	fluctuations					300:311	daily and seasonal fluctuations	281:311	daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions	281:390	The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions.
25362893	2	78	from	fluctuations	300:311	arg1	pH					316:317	pH	316:317	pH	316:317	The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions.
25362893	0	79	theme	Crassostrea	76:86	arg1	gigas					88:92	Crassostrea gigas	76:92	Crassostrea gigas	76:92	Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas.
25362893	2	80	theme	daily	281:285	arg1	fluctuations					300:311	daily and seasonal fluctuations	281:311	daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions	281:390	The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions.
25362893	1	81	theme	anthropogenic	151:163	arg1	CO2					165:167	anthropogenic CO2	151:167	increased anthropogenic CO2 emissions	141:177	BACKGROUND Ocean acidification as a result of increased anthropogenic CO2 emissions is occurring in marine and estuarine environments worldwide.
25362893	9	82	theme	energy	1637:1642	arg1	use					1653:1655	energy resource use	1637:1655	energy resource use	1637:1655	CONCLUSIONS Oyster physiology is significantly altered by exposure to elevated p CO2, indicating changes in energy resource use.
25362893	6	83	theme	acute	1037:1041	arg1	shock					1048:1052	acute heat shock	1037:1052	acute heat shock	1037:1052	Elevated p CO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock.
25362893	7	84	theme	Shotgun	1055:1061	arg1	proteomics					1063:1072	Shotgun proteomics	1055:1072	Shotgun proteomics	1055:1072	Shotgun proteomics revealed that several physiological pathways were significantly affected by ocean acidification, including antioxidant response, carbohydrate metabolism, and transcription and translation.
25362893	4	85	from	end	670:672	arg1	RESULTS					655:661	RESULTS	655:661	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments	655:745	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	1	86	theme	emissions	169:177	arg1	result					131:136	a result	129:136	a result of increased anthropogenic CO2 emissions	129:177	BACKGROUND Ocean acidification as a result of increased anthropogenic CO2 emissions is occurring in marine and estuarine environments worldwide.
25362893	0	87	from	acidification	59:71	arg1	gigas					88:92	Crassostrea gigas	76:92	Crassostrea gigas	76:92	Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas.
25362893	8	88	theme	high	1416:1419	arg1	CO2					1434:1436	high versus low p CO2	1416:1436	high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835)	1416:1526	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	10	89	from	apparent	1677:1684	arg1	assessment					1693:1702	the assessment	1689:1702	the assessment of the effects of p CO2 on the proteomic response to a second stress	1689:1771	This is especially apparent in the assessment of the effects of p CO2 on the proteomic response to a second stress.
25362893	7	90	theme	carbohydrate	1203:1214	arg1	metabolism					1216:1225	carbohydrate metabolism	1203:1225	carbohydrate metabolism	1203:1225	Shotgun proteomics revealed that several physiological pathways were significantly affected by ocean acidification, including antioxidant response, carbohydrate metabolism, and transcription and translation.
25362893	8	91	theme	low	1428:1430	arg1	CO2					1434:1436	high versus low p CO2	1416:1436	high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835)	1416:1526	Additionally, the proteomic response to a second stress differed with p CO2, with numerous processes significantly affected by mechanical stimulation at high versus low p CO2 (all proteomics data are available in the ProteomeXchange under the identifier PXD000835).
25362893	4	92	from	oysters	708:714	arg1	environments					734:745	all four p CO2 environments	719:745	all four p CO2 environments	719:745	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	1	93	theme	BACKGROUND	95:104	arg1	acidification					112:124	BACKGROUND Ocean acidification	95:124	BACKGROUND Ocean acidification as a result of increased anthropogenic CO2 emissions	95:177	BACKGROUND Ocean acidification as a result of increased anthropogenic CO2 emissions is occurring in marine and estuarine environments worldwide.
25362893	6	94	theme	p	922:922	arg1	CO2					924:926	Elevated p CO2	913:926	Elevated p CO2	913:926	Elevated p CO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock.
25362893	3	95	theme	marine	449:454	arg1	invertebrates					456:468	marine invertebrates	449:468	marine invertebrates	449:468	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	0	96	theme	physiological	27:39	arg1	response					41:48	physiological response	27:48	physiological response to ocean acidification in Crassostrea gigas	27:92	Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas.
25362893	12	97	from	effects	1961:1967	arg1	trade-offs					2028:2037	physiological trade-offs	2014:2037	oysters as well as physiological trade-offs during environmental stress	1995:2065	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	12	97	from	effects	1961:1967	arg1	oysters					1995:2001	oysters	1995:2001	oysters as well as physiological trade-offs during environmental stress	1995:2065	These data contribute to an integrative view of the effects of ocean acidification on oysters as well as physiological trade-offs during environmental stress.
25362893	3	98	theme	Crassostrea	488:498	arg1	oysters					479:485	Pacific oysters	471:485	Pacific oysters (Crassostrea gigas)	471:505	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	3	98	theme	Crassostrea	488:498	arg1	gigas					500:504	Crassostrea gigas	488:504	Crassostrea gigas	488:504	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	5	99	theme	shell	871:875	arg1	properties					849:858	micromechanical properties	833:858	micromechanical properties of the new shell	833:875	However, micromechanical properties of the new shell were compromised by elevated p CO2.
25362893	10	100	from	effects	1711:1717	arg1	response					1745:1752	the proteomic response	1731:1752	the proteomic response to a second stress	1731:1771	This is especially apparent in the assessment of the effects of p CO2 on the proteomic response to a second stress.
25362893	3	101	theme	acidification	432:444	arg1	impact					416:421	the impact	412:421	the impact of ocean acidification on marine invertebrates	412:468	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	10	102	theme	CO2	1724:1726	arg1	effects					1711:1717	the effects	1707:1717	the effects of p CO2 on the proteomic response to a second stress	1707:1771	This is especially apparent in the assessment of the effects of p CO2 on the proteomic response to a second stress.
25362893	4	103	theme	week	686:689	arg1	oysters					708:714	oysters	708:714	oysters in all four p CO2 environments	708:745	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	4	103	theme	week	686:689	arg1	period					700:705	the four week exposure period	677:705	the four week exposure period	677:705	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	11	104	theme	stress	1786:1791	arg1	response					1793:1800	The altered stress response	1774:1800	The altered stress response	1774:1800	The altered stress response illustrates that ocean acidification may impact how oysters respond to other changes in their environment.
25362893	4	105	theme	period	700:705	arg1	end					670:672	the end	666:672	the end of the four week exposure period, oysters in all four p CO2 environments	666:745	RESULTS At the end of the four week exposure period, oysters in all four p CO2 environments deposited new shell, but growth rate was not different among the treatments.
25362893	2	106	theme	open	367:370	arg1	reductions					381:390	projected end-of-century open ocean pH reductions	342:390	projected end-of-century open ocean pH reductions	342:390	The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions.
25362893	3	107	dep	exposed	512:518	arg1	μatm					597:600	800 μatm	593:600	800 μatm (pH 7.7)	593:609	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	3	107	dep	exposed	512:518	arg1	pH					584:585	pH 8.0	584:589	pH 8.0	584:589	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	3	107	dep	exposed	512:518	arg1	μatm					640:643	2800 μatm	635:643	2800 μatm (pH 7.3)	635:652	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	3	107	dep	exposed	512:518	arg1	pH					603:604	pH 7.7	603:608	pH 7.7	603:608	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	3	107	dep	exposed	512:518	arg1	pH					623:624	pH 7.6	623:628	pH 7.6	623:628	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	3	107	dep	exposed	512:518	arg1	pH					646:647	pH 7.3	646:651	pH 7.3	646:651	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	3	107	dep	exposed	512:518	arg1	μatm					578:581	400 μatm	574:581	400 μatm (pH 8.0)	574:590	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	3	107	dep	exposed	512:518	arg1	μatm					617:620	1000 μatm	612:620	1000 μatm (pH 7.6)	612:629	In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different p CO2 levels for four weeks: 400 μatm (pH 8.0), 800 μatm (pH 7.7), 1000 μatm (pH 7.6), or 2800 μatm (pH 7.3).
25362893	2	108	theme	projected	342:350	arg1	reductions					381:390	projected end-of-century open ocean pH reductions	342:390	projected end-of-century open ocean pH reductions	342:390	The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end-of-century open ocean pH reductions.
25362893	6	109	theme	fatty	956:960	arg1	composition					967:977	whole body fatty acid composition	945:977	whole body fatty acid composition	945:977	Elevated p CO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock.
28719734	9	0	theme	flap	1299:1302	arg1	layer					1311:1315	a layer	1309:1315	a layer of safranin O-positive cartilage	1309:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	0	theme	flap	1299:1302	arg1	flap					1299:1302	a vascularized platysma flap	1275:1302	a vascularized platysma flap	1275:1302	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	0	theme	flap	1299:1302	arg1	amounts					1245:1251	variable amounts	1236:1251	variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage	1236:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	0	theme	flap	1299:1302	arg1	epithelium					1263:1272	viable epithelium	1256:1272	viable epithelium	1256:1272	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	10	1	theme	viable	1492:1497	arg1	epithelium					1499:1508	a viable epithelium	1490:1508	a viable epithelium with vascular support	1490:1530	Rabbits that had undergone segmental reconstruction showed good tracheal integration, had a viable epithelium with vascular support, and the cartilage was sufficiently strong to maintain a lumen when palpated.
28719734	9	2	theme	epithelium	1263:1272	arg1	layer					1311:1315	a layer	1309:1315	a layer of safranin O-positive cartilage	1309:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	2	theme	epithelium	1263:1272	arg1	flap					1299:1302	a vascularized platysma flap	1275:1302	a vascularized platysma flap	1275:1302	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	2	theme	epithelium	1263:1272	arg1	amounts					1245:1251	variable amounts	1236:1251	variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage	1236:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	2	theme	epithelium	1263:1272	arg1	epithelium					1263:1272	viable epithelium	1256:1272	viable epithelium	1256:1272	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	7	3	theme	sound	878:882	arg1	neotracheas					884:894	structurally sound neotracheas	865:894	structurally sound neotracheas	865:894	Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound.
28719734	4	4	theme	cartilage	567:575	arg1	sheet					577:581	an engineered cartilage sheet	553:581	an engineered cartilage sheet	553:581	Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks.
28719734	3	5	theme	autologous	337:346	arg1	cells					348:352	autologous cells	337:352	autologous cells (neotracheas)	337:366	Engineered tracheal replacements composed of autologous cells (neotracheas) were tested in a New Zealand White rabbit model.
28719734	3	5	theme	autologous	337:346	arg1	neotracheas					355:365	neotracheas	355:365	neotracheas	355:365	Engineered tracheal replacements composed of autologous cells (neotracheas) were tested in a New Zealand White rabbit model.
28719734	6	6	theme	segmental	796:804	arg1	defects					815:821	segmental tracheal defects	796:821	segmental tracheal defects	796:821	In 11 cases, neotracheas deemed sufficiently strong were used to repair segmental tracheal defects.
28719734	10	7	theme	vascular	1515:1522	arg1	support					1524:1530	vascular support	1515:1530	vascular support	1515:1530	Rabbits that had undergone segmental reconstruction showed good tracheal integration, had a viable epithelium with vascular support, and the cartilage was sufficiently strong to maintain a lumen when palpated.
28719734	4	8	theme	engineered	556:565	arg1	sheet					577:581	an engineered cartilage sheet	553:581	an engineered cartilage sheet	553:581	Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks.
28719734	5	9	theme	tissue	705:710	arg1	morphology					712:721	tissue morphology	705:721	tissue morphology	705:721	In total, 28 rabbits were implanted and the neotracheas assessed for tissue morphology.
28719734	9	10	theme	variable	1236:1243	arg1	layer					1311:1315	a layer	1309:1315	a layer of safranin O-positive cartilage	1309:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	10	theme	variable	1236:1243	arg1	flap					1299:1302	a vascularized platysma flap	1275:1302	a vascularized platysma flap	1275:1302	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	10	theme	variable	1236:1243	arg1	amounts					1245:1251	variable amounts	1236:1251	variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage	1236:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	10	theme	variable	1236:1243	arg1	epithelium					1263:1272	viable epithelium	1256:1272	viable epithelium	1256:1272	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	7	11	theme	animal	962:967	arg1	handling					969:976	animal handling	962:976	animal handling	962:976	Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound.
28719734	8	12	from	place	1163:1167	arg1	left					1155:1158	left	1155:1158	left	1155:1158	Of the 15 structurally sound neotracheas, 11 were used for segmental reconstruction and were left in place for up to 21 days.
28719734	2	13	theme	replacements	278:289	arg1	fabrication					239:249	the fabrication	235:249	the fabrication of large segmental trachea replacements	235:289	The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements.
28719734	7	14	theme	cartilage	938:946	arg1	sheets					948:953	the cartilage sheets	934:953	the cartilage sheets	934:953	Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound.
28719734	0	15	theme	Tissue	0:5	arg1	engineering					7:17	Tissue engineering	0:17	Tissue engineering of a composite trachea construct	0:50	Tissue engineering of a composite trachea construct using autologous rabbit chondrocytes.
28719734	7	16	theme	success	839:845	arg1	impeded					900:906	impeded	900:906	impeded	900:906	Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound.
28719734	7	16	theme	success	839:845	arg1	rate					847:850	the success rate	835:850	the success rate of producing structurally sound neotracheas	835:894	Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound.
28719734	4	17	theme	sheet	577:581	arg1	layers					505:510	consecutive layers	493:510	consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet	493:581	Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks.
28719734	7	18	theme	sheets	948:953	arg1	disruption					920:929	physical disruption	911:929	physical disruption of the cartilage sheets during animal handling	911:976	Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound.
28719734	2	19	theme	trachea	270:276	arg1	replacements					278:289	large segmental trachea replacements	254:289	large segmental trachea replacements	254:289	The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements.
28719734	10	20	theme	tracheal	1464:1471	arg1	integration					1473:1483	good tracheal integration	1459:1483	good tracheal integration	1459:1483	Rabbits that had undergone segmental reconstruction showed good tracheal integration, had a viable epithelium with vascular support, and the cartilage was sufficiently strong to maintain a lumen when palpated.
28719734	11	21	theme	native	1758:1763	arg1	trachea					1765:1771	native trachea	1758:1771	native trachea	1758:1771	The results demonstrated that viable, trilayered, scaffold-free neotracheas could be constructed from autologous cells and could be integrated into native trachea to repair a segmental defect.
28719734	0	22	theme	construct	42:50	arg1	engineering					7:17	Tissue engineering	0:17	Tissue engineering of a composite trachea construct	0:50	Tissue engineering of a composite trachea construct using autologous rabbit chondrocytes.
28719734	8	23	from	left	1155:1158	arg1	place					1163:1167	place	1163:1167	place	1163:1167	Of the 15 structurally sound neotracheas, 11 were used for segmental reconstruction and were left in place for up to 21 days.
28719734	8	24	used	used	1112:1115	arg2	11					1104:1105	11	1104:1105	11	1104:1105	Of the 15 structurally sound neotracheas, 11 were used for segmental reconstruction and were left in place for up to 21 days.
28719734	10	25	theme	segmental	1427:1435	arg1	reconstruction					1437:1450	segmental reconstruction	1427:1450	segmental reconstruction	1427:1450	Rabbits that had undergone segmental reconstruction showed good tracheal integration, had a viable epithelium with vascular support, and the cartilage was sufficiently strong to maintain a lumen when palpated.
28719734	2	26	theme	segmental	260:268	arg1	replacements					278:289	large segmental trachea replacements	254:289	large segmental trachea replacements	254:289	The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements.
28719734	9	27	theme	endochondral	1373:1384	arg1	ossification					1386:1397	endochondral ossification	1373:1397	endochondral ossification	1373:1397	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	3	28	theme	New	385:387	arg1	model					410:414	a New Zealand White rabbit model	383:414	a New Zealand White rabbit model	383:414	Engineered tracheal replacements composed of autologous cells (neotracheas) were tested in a New Zealand White rabbit model.
28719734	4	29	theme	silicone	474:481	arg1	tube					483:486	a silicone tube	472:486	a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet	472:581	Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks.
28719734	9	30	theme	amounts	1245:1251	arg1	presence					1224:1231	the presence	1220:1231	the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage	1220:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	31	theme	ossification	1386:1397	arg1	evidence					1361:1368	evidence	1361:1368	evidence of endochondral ossification	1361:1397	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	3	32	theme	Zealand	389:395	arg1	model					410:414	a New Zealand White rabbit model	383:414	a New Zealand White rabbit model	383:414	Engineered tracheal replacements composed of autologous cells (neotracheas) were tested in a New Zealand White rabbit model.
28719734	6	33	theme	tracheal	806:813	arg1	defects					815:821	segmental tracheal defects	796:821	segmental tracheal defects	796:821	In 11 cases, neotracheas deemed sufficiently strong were used to repair segmental tracheal defects.
28719734	7	34	theme	physical	911:918	arg1	disruption					920:929	physical disruption	911:929	physical disruption of the cartilage sheets during animal handling	911:976	Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound.
28719734	4	35	theme	muscle	541:546	arg1	layers					505:510	consecutive layers	493:510	consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet	493:581	Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks.
28719734	10	36	theme	good	1459:1462	arg1	integration					1473:1483	good tracheal integration	1459:1483	good tracheal integration	1459:1483	Rabbits that had undergone segmental reconstruction showed good tracheal integration, had a viable epithelium with vascular support, and the cartilage was sufficiently strong to maintain a lumen when palpated.
28719734	10	37	with	epithelium	1499:1508	arg1	support					1524:1530	vascular support	1515:1530	vascular support	1515:1530	Rabbits that had undergone segmental reconstruction showed good tracheal integration, had a viable epithelium with vascular support, and the cartilage was sufficiently strong to maintain a lumen when palpated.
28719734	11	38	theme	segmental	1785:1793	arg1	defect					1795:1800	a segmental defect	1783:1800	a segmental defect	1783:1800	The results demonstrated that viable, trilayered, scaffold-free neotracheas could be constructed from autologous cells and could be integrated into native trachea to repair a segmental defect.
28719734	6	39	used	used	781:784	arg2	neotracheas					737:747	neotracheas	737:747	neotracheas deemed sufficiently strong	737:774	In 11 cases, neotracheas deemed sufficiently strong were used to repair segmental tracheal defects.
28719734	9	40	theme	vascularized	1277:1288	arg1	flap					1299:1302	a vascularized platysma flap	1275:1302	a vascularized platysma flap	1275:1302	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	4	41	theme	rabbit	448:453	arg1	neck					455:458	the rabbit neck	444:458	the rabbit neck	444:458	Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks.
28719734	1	42	theme	large	104:108	arg1	defects					129:135	large tracheal segmental defects	104:135	large tracheal segmental defects	104:135	The repair of large tracheal segmental defects remains an unsolved problem.
28719734	0	43	theme	trachea	34:40	arg1	construct					42:50	a composite trachea construct	22:50	a composite trachea construct	22:50	Tissue engineering of a composite trachea construct using autologous rabbit chondrocytes.
28719734	9	44	theme	platysma	1290:1297	arg1	flap					1299:1302	a vascularized platysma flap	1275:1302	a vascularized platysma flap	1275:1302	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	0	45	theme	composite	24:32	arg1	construct					42:50	a composite trachea construct	22:50	a composite trachea construct	22:50	Tissue engineering of a composite trachea construct using autologous rabbit chondrocytes.
28719734	4	46	theme	epithelium	520:529	arg1	layers					505:510	consecutive layers	493:510	consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet	493:581	Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks.
28719734	7	47	theme	neotracheas	1017:1027	arg1	15					1008:1009	15	1008:1009	15	1008:1009	Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound.
28719734	7	47	theme	neotracheas	1017:1027	arg1	neotracheas					1017:1027	18 neotracheas	1014:1027	18 neotracheas	1014:1027	Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound.
28719734	7	47	theme	neotracheas	1017:1027	arg1	%					1034:1034	83.3%	1030:1034	83.3%	1030:1034	Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound.
28719734	8	48	theme	sound	1085:1089	arg1	neotracheas					1091:1101	the 15 structurally sound neotracheas	1065:1101	the 15 structurally sound neotracheas	1065:1101	Of the 15 structurally sound neotracheas, 11 were used for segmental reconstruction and were left in place for up to 21 days.
28719734	9	49	theme	cartilage	1340:1348	arg1	layer					1311:1315	a layer	1309:1315	a layer of safranin O-positive cartilage	1309:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	49	theme	cartilage	1340:1348	arg1	flap					1299:1302	a vascularized platysma flap	1275:1302	a vascularized platysma flap	1275:1302	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	49	theme	cartilage	1340:1348	arg1	epithelium					1263:1272	viable epithelium	1256:1272	viable epithelium	1256:1272	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	8	50	theme	segmental	1121:1129	arg1	reconstruction					1131:1144	segmental reconstruction	1121:1144	segmental reconstruction	1121:1144	Of the 15 structurally sound neotracheas, 11 were used for segmental reconstruction and were left in place for up to 21 days.
28719734	3	51	theme	White	397:401	arg1	model					410:414	a New Zealand White rabbit model	383:414	a New Zealand White rabbit model	383:414	Engineered tracheal replacements composed of autologous cells (neotracheas) were tested in a New Zealand White rabbit model.
28719734	4	52	theme	skin	515:518	arg1	epithelium					520:529	skin epithelium	515:529	skin epithelium	515:529	Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks.
28719734	2	53	theme	engineering	208:218	arg1	principles					220:229	tissue engineering principles	201:229	tissue engineering principles for the fabrication of large segmental trachea replacements	201:289	The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements.
28719734	7	54	theme	study	1001:1005	arg1	end					990:992	the end	986:992	the end of the study	986:1005	Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound.
28719734	1	55	theme	tracheal	110:117	arg1	defects					129:135	large tracheal segmental defects	104:135	large tracheal segmental defects	104:135	The repair of large tracheal segmental defects remains an unsolved problem.
28719734	3	56	theme	rabbit	403:408	arg1	model					410:414	a New Zealand White rabbit model	383:414	a New Zealand White rabbit model	383:414	Engineered tracheal replacements composed of autologous cells (neotracheas) were tested in a New Zealand White rabbit model.
28719734	4	57	theme	platysma	532:539	arg1	muscle					541:546	platysma muscle	532:546	platysma muscle	532:546	Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks.
28719734	2	58	theme	tissue	201:206	arg1	principles					220:229	tissue engineering principles	201:229	tissue engineering principles for the fabrication of large segmental trachea replacements	201:289	The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements.
28719734	1	59	theme	segmental	119:127	arg1	defects					129:135	large tracheal segmental defects	104:135	large tracheal segmental defects	104:135	The repair of large tracheal segmental defects remains an unsolved problem.
28719734	9	60	theme	viable	1256:1261	arg1	epithelium					1263:1272	viable epithelium	1256:1272	viable epithelium	1256:1272	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	3	61	theme	Engineered	292:301	arg1	replacements					312:323	Engineered tracheal replacements	292:323	Engineered tracheal replacements composed of autologous cells (neotracheas)	292:366	Engineered tracheal replacements composed of autologous cells (neotracheas) were tested in a New Zealand White rabbit model.
28719734	9	62	theme	Histological	1188:1199	arg1	examination					1201:1211	Histological examination	1188:1211	Histological examination	1188:1211	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	2	63	theme	large	254:258	arg1	replacements					278:289	large segmental trachea replacements	254:289	large segmental trachea replacements	254:289	The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements.
28719734	1	64	theme	defects	129:135	arg1	repair					94:99	The repair	90:99	The repair of large tracheal segmental defects	90:135	The repair of large tracheal segmental defects remains an unsolved problem.
28719734	11	65	dep	viable	1640:1645	arg1	trilayered					1648:1657	trilayered	1648:1657	trilayered	1648:1657	The results demonstrated that viable, trilayered, scaffold-free neotracheas could be constructed from autologous cells and could be integrated into native trachea to repair a segmental defect.
28719734	11	65	dep	viable	1640:1645	arg1	scaffold-free					1660:1672	scaffold-free	1660:1672	scaffold-free	1660:1672	The results demonstrated that viable, trilayered, scaffold-free neotracheas could be constructed from autologous cells and could be integrated into native trachea to repair a segmental defect.
28719734	9	66	theme	layer	1311:1315	arg1	layer					1311:1315	a layer	1309:1315	a layer of safranin O-positive cartilage	1309:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	66	theme	layer	1311:1315	arg1	flap					1299:1302	a vascularized platysma flap	1275:1302	a vascularized platysma flap	1275:1302	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	66	theme	layer	1311:1315	arg1	amounts					1245:1251	variable amounts	1236:1251	variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage	1236:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	9	66	theme	layer	1311:1315	arg1	epithelium					1263:1272	viable epithelium	1256:1272	viable epithelium	1256:1272	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	3	67	theme	tracheal	303:310	arg1	replacements					312:323	Engineered tracheal replacements	292:323	Engineered tracheal replacements composed of autologous cells (neotracheas)	292:366	Engineered tracheal replacements composed of autologous cells (neotracheas) were tested in a New Zealand White rabbit model.
28719734	0	68	theme	rabbit	69:74	arg1	chondrocytes					76:87	autologous rabbit chondrocytes	58:87	autologous rabbit chondrocytes	58:87	Tissue engineering of a composite trachea construct using autologous rabbit chondrocytes.
28719734	2	69	theme	study	183:187	arg1	goal					170:173	The goal	166:173	The goal of this study	166:187	The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements.
28719734	11	70	theme	viable	1640:1645	arg1	neotracheas					1674:1684	viable, trilayered, scaffold-free neotracheas	1640:1684	viable, trilayered, scaffold-free neotracheas	1640:1684	The results demonstrated that viable, trilayered, scaffold-free neotracheas could be constructed from autologous cells and could be integrated into native trachea to repair a segmental defect.
28719734	10	71	dep	showed	1452:1457	arg1	had					1486:1488	had	1486:1488	had a viable epithelium with vascular support	1486:1530	Rabbits that had undergone segmental reconstruction showed good tracheal integration, had a viable epithelium with vascular support, and the cartilage was sufficiently strong to maintain a lumen when palpated.
28719734	4	72	with	tube	483:486	arg1	layers					505:510	consecutive layers	493:510	consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet	493:581	Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks.
28719734	11	73	theme	autologous	1712:1721	arg1	cells					1723:1727	autologous cells	1712:1727	autologous cells	1712:1727	The results demonstrated that viable, trilayered, scaffold-free neotracheas could be constructed from autologous cells and could be integrated into native trachea to repair a segmental defect.
28719734	9	74	theme	safranin	1320:1327	arg1	cartilage					1340:1348	safranin O-positive cartilage	1320:1348	safranin O-positive cartilage	1320:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28719734	0	75	theme	autologous	58:67	arg1	chondrocytes					76:87	autologous rabbit chondrocytes	58:87	autologous rabbit chondrocytes	58:87	Tissue engineering of a composite trachea construct using autologous rabbit chondrocytes.
28719734	4	76	theme	consecutive	493:503	arg1	layers					505:510	consecutive layers	493:510	consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet	493:581	Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle, and an engineered cartilage sheet and allowing the construct to mature for 8-12 weeks.
28719734	1	77	theme	unsolved	148:155	arg1	problem					157:163	an unsolved problem	145:163	an unsolved problem	145:163	The repair of large tracheal segmental defects remains an unsolved problem.
28719734	9	78	theme	O-positive	1329:1338	arg1	cartilage					1340:1348	safranin O-positive cartilage	1320:1348	safranin O-positive cartilage	1320:1348	Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification.
28471055	0	0	theme	capillary	85:93	arg1	column					95:100	a β-cyclodextrin/polydopamine-coated capillary column	48:100	a β-cyclodextrin/polydopamine-coated capillary column	48:100	A novel in situ strategy for the preparation of a β-cyclodextrin/polydopamine-coated capillary column for capillary electrochromatography enantioseparations.
28471055	2	1	theme	streaming	568:576	arg1	potential					578:586	streaming potential	568:586	streaming potential	568:586	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	1	2	theme	recognition	181:191	arg1	ability					193:199	the chiral recognition ability	170:199	the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine	170:269	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	0	3	theme	β-cyclodextrin/polydopamine-coated	50:83	arg1	column					95:100	a β-cyclodextrin/polydopamine-coated capillary column	48:100	a β-cyclodextrin/polydopamine-coated capillary column	48:100	A novel in situ strategy for the preparation of a β-cyclodextrin/polydopamine-coated capillary column for capillary electrochromatography enantioseparations.
28471055	7	4	theme	separation	1365:1374	arg1	efficiency					1376:1385	the separation efficiency	1361:1385	the separation efficiency of the β-cyclodextrin/polydopamine-coated capillary column	1361:1444	Moreover, the separation efficiency of the β-cyclodextrin/polydopamine-coated capillary column did not decrease obviously over 90 runs.
28471055	2	5	theme	studies	614:620	arg1	results					508:514	The results	504:514	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies	504:620	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	3	6	theme	reduced	791:797	arg1	preparation					809:819	a greatly reduced capillary preparation	781:819	a greatly reduced capillary preparation time	781:824	This coating can be achieved within 1 h affording a greatly reduced capillary preparation time.
28471055	6	7	theme	consecutive	1128:1138	arg1	runs					1140:1143	three consecutive runs	1122:1143	three consecutive runs	1122:1143	For three consecutive runs, the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability were in the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively.
28471055	0	8	theme	column	95:100	arg1	preparation					33:43	the preparation	29:43	the preparation of a β-cyclodextrin/polydopamine-coated capillary column	29:100	A novel in situ strategy for the preparation of a β-cyclodextrin/polydopamine-coated capillary column for capillary electrochromatography enantioseparations.
28471055	1	9	theme	β-cyclodextrin/polydopamine	382:408	arg1	columns					446:452	β-cyclodextrin/polydopamine composite material coated-capillary columns	382:452	β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography	382:501	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	2	10	theme	inner	695:699	arg1	wall					701:704	the inner wall	691:704	the inner wall of the capillary column	691:728	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	6	11	theme	1.03-4.18	1310:1318	arg1	range					1290:1294	the range	1286:1294	the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively	1286:1348	For three consecutive runs, the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability were in the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively.
28471055	7	12	theme	β-cyclodextrin/polydopamine-coated	1394:1427	arg1	column					1439:1444	the β-cyclodextrin/polydopamine-coated capillary column	1390:1444	the β-cyclodextrin/polydopamine-coated capillary column	1390:1444	Moreover, the separation efficiency of the β-cyclodextrin/polydopamine-coated capillary column did not decrease obviously over 90 runs.
28471055	2	13	theme	electro-osmotic	593:607	arg1	studies					614:620	electro-osmotic flow studies	593:620	electro-osmotic flow studies	593:620	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	1	14	theme	composite	410:418	arg1	columns					446:452	β-cyclodextrin/polydopamine composite material coated-capillary columns	382:452	β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography	382:501	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	6	15	theme	migration	1187:1195	arg1	times					1197:1201	the migration times	1183:1201	the migration times of the analytes for intraday, interday, and column-to-column repeatability	1183:1276	For three consecutive runs, the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability were in the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively.
28471055	8	16	theme	capillary	1605:1613	arg1	columns					1615:1621	capillary columns	1605:1621	capillary columns	1605:1621	The strategy should also be feasible to introduce and immobilize other chiral selectors on the inner walls surface of capillary columns.
28471055	6	17	theme	%	1334:1334	arg1	range					1290:1294	the range	1286:1294	the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively	1286:1348	For three consecutive runs, the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability were in the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively.
28471055	0	18	theme	capillary	106:114	arg1	electrochromatography					116:136	capillary electrochromatography	106:136	capillary electrochromatography	106:136	A novel in situ strategy for the preparation of a β-cyclodextrin/polydopamine-coated capillary column for capillary electrochromatography enantioseparations.
28471055	1	19	theme	material	420:427	arg1	columns					446:452	β-cyclodextrin/polydopamine composite material coated-capillary columns	382:452	β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography	382:501	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	2	20	theme	electron	528:535	arg1	microscopy					537:546	scanning electron microscopy	519:546	scanning electron microscopy	519:546	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	1	21	theme	β-cyclodextrin	204:217	arg1	ability					193:199	the chiral recognition ability	170:199	the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine	170:269	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	0	22	theme	novel	2:6	arg1	strategy					16:23	A novel in situ strategy	0:23	A novel in situ strategy for the preparation of a β-cyclodextrin/polydopamine-coated capillary column for capillary electrochromatography	0:136	A novel in situ strategy for the preparation of a β-cyclodextrin/polydopamine-coated capillary column for capillary electrochromatography enantioseparations.
28471055	1	23	theme	coated-capillary	429:444	arg1	columns					446:452	β-cyclodextrin/polydopamine composite material coated-capillary columns	382:452	β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography	382:501	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	2	24	theme	column	723:728	arg1	wall					701:704	the inner wall	691:704	the inner wall of the capillary column	691:728	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	5	25	theme	enantioseparation	1064:1080	arg1	efficiencies					1082:1093	Good enantioseparation efficiencies	1059:1093	Good enantioseparation efficiencies	1059:1093	Good enantioseparation efficiencies were achieved for all.
28471055	2	26	theme	scanning	519:526	arg1	microscopy					537:546	scanning electron microscopy	519:546	scanning electron microscopy	519:546	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	2	27	theme	capillary	713:721	arg1	column					723:728	the capillary column	709:728	the capillary column	709:728	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	8	28	theme	inner	1582:1586	arg1	surface					1594:1600	the inner walls surface	1578:1600	the inner walls surface of capillary columns	1578:1621	The strategy should also be feasible to introduce and immobilize other chiral selectors on the inner walls surface of capillary columns.
28471055	8	29	theme	other	1552:1556	arg1	selectors					1565:1573	other chiral selectors	1552:1573	other chiral selectors on the inner walls surface of capillary columns	1552:1621	The strategy should also be feasible to introduce and immobilize other chiral selectors on the inner walls surface of capillary columns.
28471055	4	30	theme	capillary	885:893	arg1	performance					831:841	The performance	827:841	The performance of the β-cyclodextrin/polydopamine-coated capillary	827:893	The performance of the β-cyclodextrin/polydopamine-coated capillary was validated by the analysis of seven pairs of chiral analytes, namely epinephrine, norepinephrine, isoprenaline, terbutaline, verapamil, tryptophane, carvedilol.
28471055	1	31	theme	natural	227:233	arg1	properties					244:253	the natural adhesive properties	223:253	the natural adhesive properties of polydopamine	223:269	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	0	32	theme	in	8:9	arg1	strategy					16:23	A novel in situ strategy	0:23	A novel in situ strategy for the preparation of a β-cyclodextrin/polydopamine-coated capillary column for capillary electrochromatography	0:136	A novel in situ strategy for the preparation of a β-cyclodextrin/polydopamine-coated capillary column for capillary electrochromatography enantioseparations.
28471055	1	33	theme	rapid	315:319	arg1	strategy					346:353	a rapid and in situ modification strategy	313:353	a rapid and in situ modification strategy	313:353	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	4	34	theme	β-cyclodextrin/polydopamine-coated	850:883	arg1	capillary					885:893	the β-cyclodextrin/polydopamine-coated capillary	846:893	the β-cyclodextrin/polydopamine-coated capillary	846:893	The performance of the β-cyclodextrin/polydopamine-coated capillary was validated by the analysis of seven pairs of chiral analytes, namely epinephrine, norepinephrine, isoprenaline, terbutaline, verapamil, tryptophane, carvedilol.
28471055	1	35	theme	adhesive	235:242	arg1	properties					244:253	the natural adhesive properties	223:253	the natural adhesive properties of polydopamine	223:269	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	2	36	theme	spectroscopy	554:565	arg1	results					508:514	The results	504:514	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies	504:620	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	8	37	theme	walls	1588:1592	arg1	surface					1594:1600	the inner walls surface	1578:1600	the inner walls surface of capillary columns	1578:1621	The strategy should also be feasible to introduce and immobilize other chiral selectors on the inner walls surface of capillary columns.
28471055	4	38	theme	chiral	943:948	arg1	analytes					950:957	chiral analytes	943:957	chiral analytes	943:957	The performance of the β-cyclodextrin/polydopamine-coated capillary was validated by the analysis of seven pairs of chiral analytes, namely epinephrine, norepinephrine, isoprenaline, terbutaline, verapamil, tryptophane, carvedilol.
28471055	8	39	from	selectors	1565:1573	arg1	surface					1594:1600	the inner walls surface	1578:1600	the inner walls surface of capillary columns	1578:1621	The strategy should also be feasible to introduce and immobilize other chiral selectors on the inner walls surface of capillary columns.
28471055	1	40	theme	properties	244:253	arg1	ability					193:199	the chiral recognition ability	170:199	the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine	170:269	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	2	41	theme	FTIR	549:552	arg1	spectroscopy					554:565	FTIR spectroscopy	549:565	FTIR spectroscopy	549:565	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	1	42	theme	in	325:326	arg1	strategy					346:353	a rapid and in situ modification strategy	313:353	a rapid and in situ modification strategy	313:353	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	1	43	theme	open	458:461	arg1	electrochromatography					481:501	open tubular capillary electrochromatography	458:501	open tubular capillary electrochromatography	458:501	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	6	44	theme	relative	1150:1157	arg1	deviations					1168:1177	the relative standard deviations	1146:1177	the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability	1146:1276	For three consecutive runs, the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability were in the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively.
28471055	1	45	theme	tubular	463:469	arg1	electrochromatography					481:501	open tubular capillary electrochromatography	458:501	open tubular capillary electrochromatography	458:501	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	8	46	theme	chiral	1558:1563	arg1	selectors					1565:1573	other chiral selectors	1552:1573	other chiral selectors on the inner walls surface of capillary columns	1552:1621	The strategy should also be feasible to introduce and immobilize other chiral selectors on the inner walls surface of capillary columns.
28471055	1	47	dep	in	325:326	arg1	situ					328:331	situ	328:331	situ	328:331	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	7	48	theme	capillary	1429:1437	arg1	column					1439:1444	the β-cyclodextrin/polydopamine-coated capillary column	1390:1444	the β-cyclodextrin/polydopamine-coated capillary column	1390:1444	Moreover, the separation efficiency of the β-cyclodextrin/polydopamine-coated capillary column did not decrease obviously over 90 runs.
28471055	2	49	theme	microscopy	537:546	arg1	results					508:514	The results	504:514	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies	504:620	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	1	50	theme	modification	333:344	arg1	strategy					346:353	a rapid and in situ modification strategy	313:353	a rapid and in situ modification strategy	313:353	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	1	51	theme	capillary	471:479	arg1	electrochromatography					481:501	open tubular capillary electrochromatography	458:501	open tubular capillary electrochromatography	458:501	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	4	52	theme	analytes	950:957	arg1	pairs					934:938	seven pairs	928:938	seven pairs of chiral analytes	928:957	The performance of the β-cyclodextrin/polydopamine-coated capillary was validated by the analysis of seven pairs of chiral analytes, namely epinephrine, norepinephrine, isoprenaline, terbutaline, verapamil, tryptophane, carvedilol.
28471055	7	53	theme	column	1439:1444	arg1	efficiency					1376:1385	the separation efficiency	1361:1385	the separation efficiency of the β-cyclodextrin/polydopamine-coated capillary column	1361:1444	Moreover, the separation efficiency of the β-cyclodextrin/polydopamine-coated capillary column did not decrease obviously over 90 runs.
28471055	8	54	theme	columns	1615:1621	arg1	surface					1594:1600	the inner walls surface	1578:1600	the inner walls surface of capillary columns	1578:1621	The strategy should also be feasible to introduce and immobilize other chiral selectors on the inner walls surface of capillary columns.
28471055	6	55	theme	column-to-column	1247:1262	arg1	repeatability					1264:1276	column-to-column repeatability	1247:1276	column-to-column repeatability	1247:1276	For three consecutive runs, the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability were in the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively.
28471055	1	56	theme	polydopamine	258:269	arg1	properties					244:253	the natural adhesive properties	223:253	the natural adhesive properties of polydopamine	223:269	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	1	56	theme	polydopamine	258:269	arg1	β-cyclodextrin					204:217	β-cyclodextrin	204:217	β-cyclodextrin	204:217	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	6	57	theme	0.41-1.74	1299:1307	arg1	range					1290:1294	the range	1286:1294	the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively	1286:1348	For three consecutive runs, the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability were in the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively.
28471055	0	58	dep	in	8:9	arg1	situ					11:14	situ	11:14	situ	11:14	A novel in situ strategy for the preparation of a β-cyclodextrin/polydopamine-coated capillary column for capillary electrochromatography enantioseparations.
28471055	5	59	theme	Good	1059:1062	arg1	efficiencies					1082:1093	Good enantioseparation efficiencies	1059:1093	Good enantioseparation efficiencies	1059:1093	Good enantioseparation efficiencies were achieved for all.
28471055	6	60	theme	standard	1159:1166	arg1	deviations					1168:1177	the relative standard deviations	1146:1177	the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability	1146:1276	For three consecutive runs, the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability were in the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively.
28471055	4	61	theme	pairs	934:938	arg1	analysis					916:923	the analysis	912:923	the analysis of seven pairs of chiral analytes	912:957	The performance of the β-cyclodextrin/polydopamine-coated capillary was validated by the analysis of seven pairs of chiral analytes, namely epinephrine, norepinephrine, isoprenaline, terbutaline, verapamil, tryptophane, carvedilol.
28471055	4	61	theme	pairs	934:938	arg1	epinephrine					967:977	epinephrine	967:977	epinephrine	967:977	The performance of the β-cyclodextrin/polydopamine-coated capillary was validated by the analysis of seven pairs of chiral analytes, namely epinephrine, norepinephrine, isoprenaline, terbutaline, verapamil, tryptophane, carvedilol.
28471055	6	62	theme	analytes	1210:1217	arg1	times					1197:1201	the migration times	1183:1201	the migration times of the analytes for intraday, interday, and column-to-column repeatability	1183:1276	For three consecutive runs, the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability were in the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively.
28471055	1	63	theme	alkaline	277:284	arg1	conditions					286:295	alkaline conditions	277:295	alkaline conditions	277:295	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	2	64	theme	potential	578:586	arg1	results					508:514	The results	504:514	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies	504:620	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
28471055	3	65	theme	capillary	799:807	arg1	preparation					809:819	a greatly reduced capillary preparation	781:819	a greatly reduced capillary preparation time	781:824	This coating can be achieved within 1 h affording a greatly reduced capillary preparation time.
28471055	3	66	theme	preparation	809:819	arg1	time					821:824	a greatly reduced capillary preparation time	781:824	a greatly reduced capillary preparation time	781:824	This coating can be achieved within 1 h affording a greatly reduced capillary preparation time.
28471055	1	67	theme	chiral	174:179	arg1	ability					193:199	the chiral recognition ability	170:199	the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine	170:269	Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography.
28471055	2	68	theme	flow	609:612	arg1	studies					614:620	electro-osmotic flow studies	593:620	electro-osmotic flow studies	593:620	The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column.
27387504	4	0	theme	relative	880:887	arg1	amount					889:894	the relative amount	876:894	the relative amount of trisulfated disaccharides	876:923	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	4	0	theme	relative	880:887	arg1	disaccharides					911:923	trisulfated disaccharides	899:923	trisulfated disaccharides	899:923	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	2	1	theme	core	390:393	arg1	proteins					395:402	proteoglycan core proteins	377:402	proteoglycan core proteins	377:402	Altered transcript expression of genes encoding other biosynthetic enzymes or proteoglycan core proteins could not account for the observed changes.
27387504	6	2	theme	increased	1327:1335	arg1	content					1353:1359	the increased heparan sulfate content	1323:1359	the increased heparan sulfate content	1323:1359	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	2	3	theme	proteoglycan	377:388	arg1	proteins					395:402	proteoglycan core proteins	377:402	proteoglycan core proteins	377:402	Altered transcript expression of genes encoding other biosynthetic enzymes or proteoglycan core proteins could not account for the observed changes.
27387504	6	4	theme	heparan	1337:1343	arg1	sulfate					1345:1351	heparan sulfate	1337:1351	the increased heparan sulfate content	1323:1359	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	5	5	theme	heparan	1006:1012	arg1	enzymes					1035:1041	the heparan sulfate-polymerizing enzymes	1002:1041	the heparan sulfate-polymerizing enzymes Ext1 and Ext2	1002:1055	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	5	5	theme	heparan	1006:1012	arg1	Ext2					1052:1055	Ext2	1052:1055	Ext2	1052:1055	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	5	5	theme	heparan	1006:1012	arg1	Ext1					1043:1046	Ext1	1043:1046	Ext1	1043:1046	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	4	6	theme	heparan	842:848	arg1	sulfate					850:856	heparan sulfate	842:856	heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2	842:959	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	2	7	theme	transcript	307:316	arg1	expression					318:327	Altered transcript expression	299:327	Altered transcript expression of genes encoding other biosynthetic enzymes or proteoglycan core proteins	299:402	Altered transcript expression of genes encoding other biosynthetic enzymes or proteoglycan core proteins could not account for the observed changes.
27387504	3	8	from	content	574:580	arg1	tissues					585:591	tissues	585:591	tissues isolated from Ndst2(-/-) mice	585:621	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	4	9	theme	NDST2	955:959	arg1	absence					944:950	the absence	940:950	the absence of NDST2	940:959	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	5	10	theme	expression	981:990	arg1	levels					992:997	In vivo transcript expression levels	962:997	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2	962:1055	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	4	11	from	Ndst2	800:804	arg1	sulfate					775:781	heparan sulfate	767:781	heparan sulfate from control and Ndst2(-/-) tissues	767:817	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	2	12	theme	Altered	299:305	arg1	expression					318:327	Altered transcript expression	299:327	Altered transcript expression of genes encoding other biosynthetic enzymes or proteoglycan core proteins	299:402	Altered transcript expression of genes encoding other biosynthetic enzymes or proteoglycan core proteins could not account for the observed changes.
27387504	1	13	theme	NDST1-overexpressing	271:290	arg1	cells					292:296	NDST1-overexpressing cells	271:296	NDST1-overexpressing cells	271:296	Analysis of heparan sulfate synthesized by HEK 293 cells overexpressing murine NDST1 and/or NDST2 demonstrated that the amount of heparan sulfate was increased in NDST2- but not in NDST1-overexpressing cells.
27387504	4	14	theme	structural	737:746	arg1	difference					748:757	no major structural difference	728:757	no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues	728:817	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	3	15	attach	isolated	593:600	arg2	tissues					585:591	tissues	585:591	tissues isolated from Ndst2(-/-) mice	585:621	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	3	15	attach	isolated	593:600	arg1	mice					618:621	Ndst2(-/-) mice	607:621	Ndst2(-/-) mice	607:621	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	4	16	theme	sulfate	850:856	arg1	exception					829:837	the exception	825:837	the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2	825:959	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	4	17	from	control	788:794	arg1	sulfate					775:781	heparan sulfate	767:781	heparan sulfate from control and Ndst2(-/-) tissues	767:817	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	3	18	theme	heparan	558:564	arg1	sulfate					566:572	heparan sulfate	558:572	heparan sulfate content	558:580	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	7	19	theme	chain	1488:1492	arg1	length					1494:1499	increased heparan sulfate chain length	1462:1499	increased heparan sulfate chain length	1462:1499	A model is discussed where NDST2-specific substrate modification stimulates elongation resulting in increased heparan sulfate chain length.
27387504	7	20	theme	NDST2-specific	1389:1402	arg1	modification					1414:1425	NDST2-specific substrate modification	1389:1425	NDST2-specific substrate modification	1389:1425	A model is discussed where NDST2-specific substrate modification stimulates elongation resulting in increased heparan sulfate chain length.
27387504	5	21	dep	other	1136:1140	arg1	than					1142:1145	than	1142:1145	than	1142:1145	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	4	22	dep	control	788:794	arg1	tissues					811:817	tissues	811:817	tissues	811:817	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	6	23	theme	Size	1177:1180	arg1	estimation					1182:1191	Size estimation	1177:1191	Size estimation of heparan sulfate polysaccharide chains	1177:1232	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	4	24	from	spleen	863:868	arg1	exception					829:837	the exception	825:837	the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2	825:959	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	4	24	from	spleen	863:868	arg1	sulfate					850:856	heparan sulfate	842:856	heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2	842:959	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	4	25	theme	disaccharide	687:698	arg1	analysis					712:719	Detailed disaccharide composition analysis	678:719	Detailed disaccharide composition analysis	678:719	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	0	26	theme	NDST2	0:4	arg1	Enzyme					43:48	NDST2 (N-Deacetylase/N-Sulfotransferase-2) Enzyme	0:48	NDST2 (N-Deacetylase/N-Sulfotransferase-2) Enzyme	0:48	NDST2 (N-Deacetylase/N-Sulfotransferase-2) Enzyme Regulates Heparan Sulfate Chain Length.
27387504	6	27	theme	chains	1227:1232	arg1	estimation					1182:1191	Size estimation	1177:1191	Size estimation of heparan sulfate polysaccharide chains	1177:1232	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	6	28	theme	NDST2-overexpressing	1276:1295	arg1	cells					1297:1301	NDST2-overexpressing cells	1276:1301	NDST2-overexpressing cells alone	1276:1307	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	2	29	theme	genes	332:336	arg1	expression					318:327	Altered transcript expression	299:327	Altered transcript expression of genes encoding other biosynthetic enzymes or proteoglycan core proteins	299:402	Altered transcript expression of genes encoding other biosynthetic enzymes or proteoglycan core proteins could not account for the observed changes.
27387504	4	30	theme	Detailed	678:685	arg1	analysis					712:719	Detailed disaccharide composition analysis	678:719	Detailed disaccharide composition analysis	678:719	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	4	31	theme	heparan	767:773	arg1	sulfate					775:781	heparan sulfate	767:781	heparan sulfate from control and Ndst2(-/-) tissues	767:817	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	7	32	theme	substrate	1404:1412	arg1	modification					1414:1425	NDST2-specific substrate modification	1389:1425	NDST2-specific substrate modification	1389:1425	A model is discussed where NDST2-specific substrate modification stimulates elongation resulting in increased heparan sulfate chain length.
27387504	1	33	theme	sulfate	110:116	arg1	Analysis					90:97	Analysis	90:97	Analysis of heparan sulfate synthesized by HEK 293 cells overexpressing murine NDST1 and/or NDST2	90:186	Analysis of heparan sulfate synthesized by HEK 293 cells overexpressing murine NDST1 and/or NDST2 demonstrated that the amount of heparan sulfate was increased in NDST2- but not in NDST1-overexpressing cells.
27387504	5	34	theme	transcript	970:979	arg1	levels					992:997	In vivo transcript expression levels	962:997	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2	962:1055	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	1	35	theme	heparan	102:108	arg1	sulfate					110:116	heparan sulfate	102:116	heparan sulfate synthesized by HEK 293 cells overexpressing murine NDST1 and/or NDST2	102:186	Analysis of heparan sulfate synthesized by HEK 293 cells overexpressing murine NDST1 and/or NDST2 demonstrated that the amount of heparan sulfate was increased in NDST2- but not in NDST1-overexpressing cells.
27387504	6	36	theme	sulfate	1204:1210	arg1	chains					1227:1232	heparan sulfate polysaccharide chains	1196:1232	heparan sulfate polysaccharide chains	1196:1232	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	5	37	theme	NDST2	1089:1093	arg1	levels					1095:1100	NDST2 levels	1089:1100	NDST2 levels	1089:1100	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	7	38	theme	sulfate	1480:1486	arg1	length					1494:1499	increased heparan sulfate chain length	1462:1499	increased heparan sulfate chain length	1462:1499	A model is discussed where NDST2-specific substrate modification stimulates elongation resulting in increased heparan sulfate chain length.
27387504	5	39	dep	enzymes	1035:1041	arg1	enzymes					1035:1041	the heparan sulfate-polymerizing enzymes	1002:1041	the heparan sulfate-polymerizing enzymes Ext1 and Ext2	1002:1055	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	5	39	dep	enzymes	1035:1041	arg1	Ext2					1052:1055	Ext2	1052:1055	Ext2	1052:1055	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	5	39	dep	enzymes	1035:1041	arg1	Ext1					1043:1046	Ext1	1043:1046	Ext1	1043:1046	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	5	40	theme	increased	1147:1155	arg1	transcription					1162:1174	increased gene transcription	1147:1174	regulation other than increased gene transcription	1125:1174	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	3	41	theme	sulfate	511:517	arg1	amount					493:498	the amount	489:498	the amount of heparan sulfate synthesized	489:529	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	3	41	theme	sulfate	511:517	arg1	sulfate					511:517	heparan sulfate	503:517	heparan sulfate synthesized	503:529	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	6	42	theme	chain	1259:1263	arg1	lengths					1265:1271	increased chain lengths	1249:1271	increased chain lengths in NDST2-overexpressing cells alone	1249:1307	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	3	43	theme	NDST2	469:473	arg1	role					461:464	the role	457:464	the role of NDST2 in regulating the amount of heparan sulfate synthesized	457:529	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	3	44	theme	-/-	613:615	arg1	mice					618:621	Ndst2(-/-) mice	607:621	Ndst2(-/-) mice	607:621	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	6	45	theme	polysaccharide	1212:1225	arg1	chains					1227:1232	heparan sulfate polysaccharide chains	1196:1232	heparan sulfate polysaccharide chains	1196:1232	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	1	46	theme	heparan	220:226	arg1	sulfate					228:234	heparan sulfate	220:234	heparan sulfate	220:234	Analysis of heparan sulfate synthesized by HEK 293 cells overexpressing murine NDST1 and/or NDST2 demonstrated that the amount of heparan sulfate was increased in NDST2- but not in NDST1-overexpressing cells.
27387504	5	47	theme	gene	1157:1160	arg1	transcription					1162:1174	increased gene transcription	1147:1174	regulation other than increased gene transcription	1125:1174	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	7	48	theme	heparan	1472:1478	arg1	sulfate					1480:1486	heparan sulfate	1472:1486	increased heparan sulfate chain length	1462:1499	A model is discussed where NDST2-specific substrate modification stimulates elongation resulting in increased heparan sulfate chain length.
27387504	3	49	theme	reduced	640:646	arg1	levels					648:653	reduced levels	640:653	reduced levels of the polysaccharide	640:675	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	7	50	theme	increased	1462:1470	arg1	length					1494:1499	increased heparan sulfate chain length	1462:1499	increased heparan sulfate chain length	1462:1499	A model is discussed where NDST2-specific substrate modification stimulates elongation resulting in increased heparan sulfate chain length.
27387504	1	51	theme	sulfate	228:234	arg1	amount					210:215	the amount	206:215	the amount of heparan sulfate	206:234	Analysis of heparan sulfate synthesized by HEK 293 cells overexpressing murine NDST1 and/or NDST2 demonstrated that the amount of heparan sulfate was increased in NDST2- but not in NDST1-overexpressing cells.
27387504	1	51	theme	sulfate	228:234	arg1	sulfate					228:234	heparan sulfate	220:234	heparan sulfate	220:234	Analysis of heparan sulfate synthesized by HEK 293 cells overexpressing murine NDST1 and/or NDST2 demonstrated that the amount of heparan sulfate was increased in NDST2- but not in NDST1-overexpressing cells.
27387504	0	52	theme	Sulfate	68:74	arg1	Length					82:87	Heparan Sulfate Chain Length	60:87	Heparan Sulfate Chain Length	60:87	NDST2 (N-Deacetylase/N-Sulfotransferase-2) Enzyme Regulates Heparan Sulfate Chain Length.
27387504	5	53	theme	transcription	1162:1174	arg1	mode					1117:1120	a mode	1115:1120	a mode of regulation other than increased gene transcription	1115:1174	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	4	54	theme	disaccharides	911:923	arg1	amount					889:894	the relative amount	876:894	the relative amount of trisulfated disaccharides	876:923	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	4	54	theme	disaccharides	911:923	arg1	disaccharides					911:923	trisulfated disaccharides	899:923	trisulfated disaccharides	899:923	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	3	55	theme	Ndst2	607:611	arg1	mice					618:621	Ndst2(-/-) mice	607:621	Ndst2(-/-) mice	607:621	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	3	56	theme	heparan	503:509	arg1	sulfate					511:517	heparan sulfate	503:517	heparan sulfate synthesized	503:529	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	0	57	theme	Heparan	60:66	arg1	Length					82:87	Heparan Sulfate Chain Length	60:87	Heparan Sulfate Chain Length	60:87	NDST2 (N-Deacetylase/N-Sulfotransferase-2) Enzyme Regulates Heparan Sulfate Chain Length.
27387504	6	58	theme	heparan	1196:1202	arg1	chains					1227:1232	heparan sulfate polysaccharide chains	1196:1232	heparan sulfate polysaccharide chains	1196:1232	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	4	59	theme	trisulfated	899:909	arg1	disaccharides					911:923	trisulfated disaccharides	899:923	trisulfated disaccharides	899:923	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	6	60	theme	increased	1249:1257	arg1	lengths					1265:1271	increased chain lengths	1249:1271	increased chain lengths in NDST2-overexpressing cells alone	1249:1307	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	4	61	from	exception	829:837	arg1	spleen					863:868	spleen	863:868	spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2	863:959	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	1	62	theme	HEK	133:135	arg1	cells					141:145	HEK 293 cells	133:145	HEK 293 cells overexpressing murine NDST1 and/or NDST2	133:186	Analysis of heparan sulfate synthesized by HEK 293 cells overexpressing murine NDST1 and/or NDST2 demonstrated that the amount of heparan sulfate was increased in NDST2- but not in NDST1-overexpressing cells.
27387504	5	63	theme	enzymes	1035:1041	arg1	levels					992:997	In vivo transcript expression levels	962:997	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2	962:1055	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	4	64	theme	composition	700:710	arg1	analysis					712:719	Detailed disaccharide composition analysis	678:719	Detailed disaccharide composition analysis	678:719	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27387504	2	65	theme	biosynthetic	353:364	arg1	enzymes					366:372	other biosynthetic enzymes	347:372	other biosynthetic enzymes	347:372	Altered transcript expression of genes encoding other biosynthetic enzymes or proteoglycan core proteins could not account for the observed changes.
27387504	5	66	theme	In	962:963	arg1	levels					992:997	In vivo transcript expression levels	962:997	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2	962:1055	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	6	67	theme	sulfate	1345:1351	arg1	content					1353:1359	the increased heparan sulfate content	1323:1359	the increased heparan sulfate content	1323:1359	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	3	68	theme	polysaccharide	662:675	arg1	levels					648:653	reduced levels	640:653	reduced levels of the polysaccharide	640:675	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	3	69	theme	sulfate	566:572	arg1	content					574:580	heparan sulfate content	558:580	heparan sulfate content	558:580	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	2	70	theme	other	347:351	arg1	enzymes					366:372	other biosynthetic enzymes	347:372	other biosynthetic enzymes	347:372	Altered transcript expression of genes encoding other biosynthetic enzymes or proteoglycan core proteins could not account for the observed changes.
27387504	2	71	theme	observed	430:437	arg1	changes					439:445	the observed changes	426:445	the observed changes	426:445	Altered transcript expression of genes encoding other biosynthetic enzymes or proteoglycan core proteins could not account for the observed changes.
27387504	5	72	theme	sulfate-polymerizing	1014:1033	arg1	enzymes					1035:1041	the heparan sulfate-polymerizing enzymes	1002:1041	the heparan sulfate-polymerizing enzymes Ext1 and Ext2	1002:1055	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	5	72	theme	sulfate-polymerizing	1014:1033	arg1	Ext2					1052:1055	Ext2	1052:1055	Ext2	1052:1055	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	5	72	theme	sulfate-polymerizing	1014:1033	arg1	Ext1					1043:1046	Ext1	1043:1046	Ext1	1043:1046	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	5	73	dep	In	962:963	arg1	vivo					965:968	vivo	965:968	vivo	965:968	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	6	74	from	lengths	1265:1271	arg1	cells					1297:1301	NDST2-overexpressing cells	1276:1301	NDST2-overexpressing cells alone	1276:1307	Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content.
27387504	3	75	contain	contained	630:638	arg1	content					574:580	heparan sulfate content	558:580	heparan sulfate content	558:580	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	3	75	contain	contained	630:638	arg2	levels					648:653	reduced levels	640:653	reduced levels of the polysaccharide	640:675	However, the role of NDST2 in regulating the amount of heparan sulfate synthesized was confirmed by analyzing heparan sulfate content in tissues isolated from Ndst2(-/-) mice, which contained reduced levels of the polysaccharide.
27387504	0	76	theme	Chain	76:80	arg1	Length					82:87	Heparan Sulfate Chain Length	60:87	Heparan Sulfate Chain Length	60:87	NDST2 (N-Deacetylase/N-Sulfotransferase-2) Enzyme Regulates Heparan Sulfate Chain Length.
27387504	5	77	theme	regulation	1125:1134	arg1	mode					1117:1120	a mode	1115:1120	a mode of regulation other than increased gene transcription	1115:1174	In vivo transcript expression levels of the heparan sulfate-polymerizing enzymes Ext1 and Ext2 were also largely unaffected by NDST2 levels, pointing to a mode of regulation other than increased gene transcription.
27387504	0	78	theme	N-Deacetylase/N-Sulfotransferase-2	7:40	arg1	Enzyme					43:48	NDST2 (N-Deacetylase/N-Sulfotransferase-2) Enzyme	0:48	NDST2 (N-Deacetylase/N-Sulfotransferase-2) Enzyme	0:48	NDST2 (N-Deacetylase/N-Sulfotransferase-2) Enzyme Regulates Heparan Sulfate Chain Length.
27387504	1	79	theme	murine	162:167	arg1	NDST1					169:173	murine NDST1	162:173	murine NDST1	162:173	Analysis of heparan sulfate synthesized by HEK 293 cells overexpressing murine NDST1 and/or NDST2 demonstrated that the amount of heparan sulfate was increased in NDST2- but not in NDST1-overexpressing cells.
27387504	4	80	theme	major	731:735	arg1	difference					748:757	no major structural difference	728:757	no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues	728:817	Detailed disaccharide composition analysis showed no major structural difference between heparan sulfate from control and Ndst2(-/-) tissues, with the exception of heparan sulfate from spleen where the relative amount of trisulfated disaccharides was lowered in the absence of NDST2.
27261748	5	0	from	changes	988:994	arg1	capacity					1014:1021	the adsorption capacity	999:1021	the adsorption capacity	999:1021	After five adsorption-desorption cycles, no significant changes in the adsorption capacity were observed, suggesting its excellent reusability.
27261748	1	1	theme	phase	288:292	arg1	emulsions					294:302	high internal phase emulsions	274:302	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	1	1	theme	phase	288:292	arg1	HIPEs					305:309	HIPEs	305:309	HIPEs	305:309	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	6	2	theme	metals	1236:1241	arg1	removal					1219:1225	highly efficient removal	1202:1225	highly efficient removal of heavy metals	1202:1241	The magnetic porous sphere can be easily separated from the solution and then find its potential as a recyclable material for highly efficient removal of heavy metals.
27261748	3	3	theme	magnetic	559:566	arg1	sphere					575:580	magnetic porous sphere	559:580	magnetic porous sphere	559:580	The results indicated that the silanized Fe3O4 can influence greatly the pore structure of magnetic porous sphere in addition to non-negligible impacts of the proportion of mixed solvent and co-surfactant.
27261748	0	4	theme	spheres	76:82	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+)	0:147	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	0	4	theme	spheres	76:82	arg1	Cd					153:154	Cd	153:154	Cd(2.)	153:158	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	1	5	theme	emulsions	294:302	arg1	polymerization					337:350	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	6	6	theme	heavy	1230:1234	arg1	metals					1236:1241	heavy metals	1230:1241	heavy metals	1230:1241	The magnetic porous sphere can be easily separated from the solution and then find its potential as a recyclable material for highly efficient removal of heavy metals.
27261748	6	7	theme	magnetic	1080:1087	arg1	sphere					1096:1101	The magnetic porous sphere	1076:1101	The magnetic porous sphere	1076:1101	The magnetic porous sphere can be easily separated from the solution and then find its potential as a recyclable material for highly efficient removal of heavy metals.
27261748	1	8	theme	acrylic	213:219	arg1	cellulose-g-poly					196:211	magnetic hydroxypropyl cellulose-g-poly	173:211	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	173:240	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	1	8	theme	acrylic	213:219	arg1	acid					221:224	acrylic acid	213:224	acrylic acid	213:224	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	0	9	theme	high	98:101	arg1	emulsion					118:125	Pickering high internal phase emulsion	88:125	Pickering high internal phase emulsion for removal of Cu(2+)	88:147	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	3	10	theme	mixed	641:645	arg1	solvent					647:653	mixed solvent	641:653	mixed solvent	641:653	The results indicated that the silanized Fe3O4 can influence greatly the pore structure of magnetic porous sphere in addition to non-negligible impacts of the proportion of mixed solvent and co-surfactant.
27261748	4	11	theme	adsorption	911:920	arg1	capacity					922:929	high adsorption capacity	906:929	high adsorption capacity	906:929	The adsorption experiment demonstrated that the adsorption equilibrium can be reached within 40min and the maximal adsorption capacity was 300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+), suggesting its fast adsorption kinetics and high adsorption capacity.
27261748	0	12	theme	Pickering	88:96	arg1	emulsion					118:125	Pickering high internal phase emulsion	88:125	Pickering high internal phase emulsion for removal of Cu(2+)	88:147	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	4	13	theme	adsorption	722:731	arg1	equilibrium					733:743	the adsorption equilibrium	718:743	the adsorption equilibrium	718:743	The adsorption experiment demonstrated that the adsorption equilibrium can be reached within 40min and the maximal adsorption capacity was 300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+), suggesting its fast adsorption kinetics and high adsorption capacity.
27261748	0	14	theme	phase	112:116	arg1	emulsion					118:125	Pickering high internal phase emulsion	88:125	Pickering high internal phase emulsion for removal of Cu(2+)	88:147	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	6	15	theme	efficient	1209:1217	arg1	removal					1219:1225	highly efficient removal	1202:1225	highly efficient removal of heavy metals	1202:1241	The magnetic porous sphere can be easily separated from the solution and then find its potential as a recyclable material for highly efficient removal of heavy metals.
27261748	0	16	theme	internal	103:110	arg1	emulsion					118:125	Pickering high internal phase emulsion	88:125	Pickering high internal phase emulsion for removal of Cu(2+)	88:147	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	1	17	theme	integrated	312:321	arg1	polymerization					337:350	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	2	18	theme	structures	412:421	arg1	composition					371:381	composition	371:381	composition	371:381	The structure and composition of modified Fe3O4 and porous structures were characterized by TEM, XRD, TGA and SEM.
27261748	2	18	theme	structures	412:421	arg1	structure					357:365	structure	357:365	structure	357:365	The structure and composition of modified Fe3O4 and porous structures were characterized by TEM, XRD, TGA and SEM.
27261748	1	19	theme	precipitation	323:335	arg1	polymerization					337:350	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	4	20	theme	high	906:909	arg1	capacity					922:929	high adsorption capacity	906:929	high adsorption capacity	906:929	The adsorption experiment demonstrated that the adsorption equilibrium can be reached within 40min and the maximal adsorption capacity was 300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+), suggesting its fast adsorption kinetics and high adsorption capacity.
27261748	4	21	theme	fast	877:880	arg1	kinetics					893:900	its fast adsorption kinetics	873:900	its fast adsorption kinetics	873:900	The adsorption experiment demonstrated that the adsorption equilibrium can be reached within 40min and the maximal adsorption capacity was 300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+), suggesting its fast adsorption kinetics and high adsorption capacity.
27261748	2	22	theme	porous	405:410	arg1	structures					412:421	porous structures	405:421	porous structures	405:421	The structure and composition of modified Fe3O4 and porous structures were characterized by TEM, XRD, TGA and SEM.
27261748	1	23	theme	porous	227:232	arg1	spheres					234:240	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	173:240	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	173:240	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	0	24	theme	hydroxypropyl	24:36	arg1	cellulose-g-poly					38:53	magnetic hydroxypropyl cellulose-g-poly	15:53	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	15:82	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	0	24	theme	hydroxypropyl	24:36	arg1	acid					63:66	acrylic acid	55:66	acrylic acid	55:66	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	1	25	theme	spheres	234:240	arg1	series					163:168	A series	161:168	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	161:240	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	5	26	theme	significant	976:986	arg1	changes					988:994	no significant changes	973:994	no significant changes in the adsorption capacity	973:1021	After five adsorption-desorption cycles, no significant changes in the adsorption capacity were observed, suggesting its excellent reusability.
27261748	3	27	theme	silanized	499:507	arg1	Fe3O4					509:513	the silanized Fe3O4	495:513	the silanized Fe3O4	495:513	The results indicated that the silanized Fe3O4 can influence greatly the pore structure of magnetic porous sphere in addition to non-negligible impacts of the proportion of mixed solvent and co-surfactant.
27261748	4	28	theme	maximal	781:787	arg1	300.00mg/g					813:822	300.00mg/g	813:822	300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+)	813:859	The adsorption experiment demonstrated that the adsorption equilibrium can be reached within 40min and the maximal adsorption capacity was 300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+), suggesting its fast adsorption kinetics and high adsorption capacity.
27261748	4	28	theme	maximal	781:787	arg1	capacity					800:807	the maximal adsorption capacity	777:807	the maximal adsorption capacity	777:807	The adsorption experiment demonstrated that the adsorption equilibrium can be reached within 40min and the maximal adsorption capacity was 300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+), suggesting its fast adsorption kinetics and high adsorption capacity.
27261748	2	29	theme	modified	386:393	arg1	Fe3O4					395:399	modified Fe3O4	386:399	modified Fe3O4	386:399	The structure and composition of modified Fe3O4 and porous structures were characterized by TEM, XRD, TGA and SEM.
27261748	0	30	theme	magnetic	15:22	arg1	cellulose-g-poly					38:53	magnetic hydroxypropyl cellulose-g-poly	15:53	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	15:82	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	0	30	theme	magnetic	15:22	arg1	acid					63:66	acrylic acid	55:66	acrylic acid	55:66	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	5	31	theme	excellent	1053:1061	arg1	reusability					1063:1073	its excellent reusability	1049:1073	its excellent reusability	1049:1073	After five adsorption-desorption cycles, no significant changes in the adsorption capacity were observed, suggesting its excellent reusability.
27261748	5	32	theme	adsorption-desorption	943:963	arg1	cycles					965:970	five adsorption-desorption cycles	938:970	five adsorption-desorption cycles	938:970	After five adsorption-desorption cycles, no significant changes in the adsorption capacity were observed, suggesting its excellent reusability.
27261748	3	33	theme	non-negligible	597:610	arg1	impacts					612:618	non-negligible impacts	597:618	non-negligible impacts of the proportion of mixed solvent and co-surfactant	597:671	The results indicated that the silanized Fe3O4 can influence greatly the pore structure of magnetic porous sphere in addition to non-negligible impacts of the proportion of mixed solvent and co-surfactant.
27261748	3	34	theme	pore	541:544	arg1	structure					546:554	the pore structure	537:554	the pore structure of magnetic porous sphere	537:580	The results indicated that the silanized Fe3O4 can influence greatly the pore structure of magnetic porous sphere in addition to non-negligible impacts of the proportion of mixed solvent and co-surfactant.
27261748	0	35	dep	Cd	153:154	arg1	2					156:156	2	156:156	2	156:156	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	0	36	theme	cellulose-g-poly	38:53	arg1	spheres					76:82	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	15:82	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	15:82	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	4	37	theme	adsorption	882:891	arg1	kinetics					893:900	its fast adsorption kinetics	873:900	its fast adsorption kinetics	873:900	The adsorption experiment demonstrated that the adsorption equilibrium can be reached within 40min and the maximal adsorption capacity was 300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+), suggesting its fast adsorption kinetics and high adsorption capacity.
27261748	1	38	theme	O/W	260:262	arg1	polymerization					337:350	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	3	39	theme	co-surfactant	659:671	arg1	proportion					627:636	the proportion	623:636	the proportion of mixed solvent and co-surfactant	623:671	The results indicated that the silanized Fe3O4 can influence greatly the pore structure of magnetic porous sphere in addition to non-negligible impacts of the proportion of mixed solvent and co-surfactant.
27261748	6	40	theme	porous	1089:1094	arg1	sphere					1096:1101	The magnetic porous sphere	1076:1101	The magnetic porous sphere	1076:1101	The magnetic porous sphere can be easily separated from the solution and then find its potential as a recyclable material for highly efficient removal of heavy metals.
27261748	0	41	theme	acrylic	55:61	arg1	cellulose-g-poly					38:53	magnetic hydroxypropyl cellulose-g-poly	15:53	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	15:82	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	0	41	theme	acrylic	55:61	arg1	acid					63:66	acrylic acid	55:66	acrylic acid	55:66	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	1	42	theme	Pickering	264:272	arg1	polymerization					337:350	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	0	43	theme	Cu	142:143	arg1	removal					131:137	removal	131:137	removal of Cu(2+)	131:147	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	4	44	theme	adsorption	789:798	arg1	300.00mg/g					813:822	300.00mg/g	813:822	300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+)	813:859	The adsorption experiment demonstrated that the adsorption equilibrium can be reached within 40min and the maximal adsorption capacity was 300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+), suggesting its fast adsorption kinetics and high adsorption capacity.
27261748	4	44	theme	adsorption	789:798	arg1	capacity					800:807	the maximal adsorption capacity	777:807	the maximal adsorption capacity	777:807	The adsorption experiment demonstrated that the adsorption equilibrium can be reached within 40min and the maximal adsorption capacity was 300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+), suggesting its fast adsorption kinetics and high adsorption capacity.
27261748	3	45	theme	solvent	647:653	arg1	proportion					627:636	the proportion	623:636	the proportion of mixed solvent and co-surfactant	623:671	The results indicated that the silanized Fe3O4 can influence greatly the pore structure of magnetic porous sphere in addition to non-negligible impacts of the proportion of mixed solvent and co-surfactant.
27261748	1	46	theme	high	274:277	arg1	emulsions					294:302	high internal phase emulsions	274:302	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	1	46	theme	high	274:277	arg1	HIPEs					305:309	HIPEs	305:309	HIPEs	305:309	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	6	47	theme	recyclable	1178:1187	arg1	material					1189:1196	a recyclable material	1176:1196	a recyclable material	1176:1196	The magnetic porous sphere can be easily separated from the solution and then find its potential as a recyclable material for highly efficient removal of heavy metals.
27261748	3	48	theme	proportion	627:636	arg1	impacts					612:618	non-negligible impacts	597:618	non-negligible impacts of the proportion of mixed solvent and co-surfactant	597:671	The results indicated that the silanized Fe3O4 can influence greatly the pore structure of magnetic porous sphere in addition to non-negligible impacts of the proportion of mixed solvent and co-surfactant.
27261748	3	49	theme	porous	568:573	arg1	sphere					575:580	magnetic porous sphere	559:580	magnetic porous sphere	559:580	The results indicated that the silanized Fe3O4 can influence greatly the pore structure of magnetic porous sphere in addition to non-negligible impacts of the proportion of mixed solvent and co-surfactant.
27261748	4	50	theme	adsorption	678:687	arg1	experiment					689:698	The adsorption experiment	674:698	The adsorption experiment	674:698	The adsorption experiment demonstrated that the adsorption equilibrium can be reached within 40min and the maximal adsorption capacity was 300.00mg/g for Cd(2+) and 242.72mg/g for Cu(2+), suggesting its fast adsorption kinetics and high adsorption capacity.
27261748	3	51	theme	sphere	575:580	arg1	structure					546:554	the pore structure	537:554	the pore structure of magnetic porous sphere	537:580	The results indicated that the silanized Fe3O4 can influence greatly the pore structure of magnetic porous sphere in addition to non-negligible impacts of the proportion of mixed solvent and co-surfactant.
27261748	1	52	theme	magnetic	173:180	arg1	cellulose-g-poly					196:211	magnetic hydroxypropyl cellulose-g-poly	173:211	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	173:240	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	1	52	theme	magnetic	173:180	arg1	acid					221:224	acrylic acid	213:224	acrylic acid	213:224	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	0	53	theme	porous	69:74	arg1	spheres					76:82	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	15:82	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	15:82	Fabrication of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres via Pickering high internal phase emulsion for removal of Cu(2+) and Cd(2.)
27261748	5	54	theme	adsorption	1003:1012	arg1	capacity					1014:1021	the adsorption capacity	999:1021	the adsorption capacity	999:1021	After five adsorption-desorption cycles, no significant changes in the adsorption capacity were observed, suggesting its excellent reusability.
27261748	2	55	dep	structure	357:365	arg1	The					353:355	The	353:355	The	353:355	The structure and composition of modified Fe3O4 and porous structures were characterized by TEM, XRD, TGA and SEM.
27261748	1	56	theme	hydroxypropyl	182:194	arg1	cellulose-g-poly					196:211	magnetic hydroxypropyl cellulose-g-poly	173:211	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	173:240	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	1	56	theme	hydroxypropyl	182:194	arg1	acid					221:224	acrylic acid	213:224	acrylic acid	213:224	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	1	57	theme	internal	279:286	arg1	emulsions					294:302	high internal phase emulsions	274:302	O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization	260:350	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	1	57	theme	internal	279:286	arg1	HIPEs					305:309	HIPEs	305:309	HIPEs	305:309	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
27261748	2	58	theme	Fe3O4	395:399	arg1	composition					371:381	composition	371:381	composition	371:381	The structure and composition of modified Fe3O4 and porous structures were characterized by TEM, XRD, TGA and SEM.
27261748	2	58	theme	Fe3O4	395:399	arg1	structure					357:365	structure	357:365	structure	357:365	The structure and composition of modified Fe3O4 and porous structures were characterized by TEM, XRD, TGA and SEM.
27261748	1	59	theme	cellulose-g-poly	196:211	arg1	spheres					234:240	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	173:240	magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres	173:240	A series of magnetic hydroxypropyl cellulose-g-poly(acrylic acid) porous spheres were prepared via O/W Pickering high internal phase emulsions (HIPEs) integrated precipitation polymerization.
25133925	0	0	theme	fluorescent	81:91	arg1	surface					114:120	fluorescent and patterned device surface	81:120	fluorescent and patterned device surface	81:120	Electroaddressing of ZnS quantum dots by codeposition with chitosan to construct fluorescent and patterned device surface.
25133925	1	1	theme	diverse	372:378	arg1	range					380:384	a diverse range	370:384	a diverse range of applications	370:400	Electroaddressing is an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control, and it also offers a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications.
25133925	4	2	theme	UV	737:738	arg1	light					740:744	UV light	737:744	UV light	737:744	It is shown that the deposited surface displays clear fluorescence under UV light, and the fluorescence intensity of the surface can be controlled by electrodeposition conditions (e.g., deposition time).
25133925	6	3	theme	spatiotemporal	1031:1044	arg1	selectivity					1046:1056	the spatiotemporal selectivity	1027:1056	the spatiotemporal selectivity of electroaddressing	1027:1077	Specifically, taking advantage of the spatiotemporal selectivity of electroaddressing and the pH-responsive property of chitosan, we construct diverse fluorescent patterns by electroaddressing QDs/chitosan composite at the localized region.
25133925	7	4	theme	QDs/chitosan	1284:1295	arg1	composite					1297:1305	QDs/chitosan composite	1284:1305	QDs/chitosan composite	1284:1305	It is also found that the fluorescent patterns of QDs/chitosan composite have reproducibility.
25133925	6	5	theme	diverse	1136:1142	arg1	patterns					1156:1163	diverse fluorescent patterns	1136:1163	diverse fluorescent patterns	1136:1163	Specifically, taking advantage of the spatiotemporal selectivity of electroaddressing and the pH-responsive property of chitosan, we construct diverse fluorescent patterns by electroaddressing QDs/chitosan composite at the localized region.
25133925	3	6	from	surfaces	568:575	arg1	electrodes					614:623	the electrodes	610:623	the electrodes	610:623	First, the surfaces of ZnS QDs/chitosan composite on the electrodes are built by electrodeposition method.
25133925	0	7	theme	patterned	97:105	arg1	surface					114:120	fluorescent and patterned device surface	81:120	fluorescent and patterned device surface	81:120	Electroaddressing of ZnS quantum dots by codeposition with chitosan to construct fluorescent and patterned device surface.
25133925	8	8	theme	fluorescent	1416:1426	arg1	surface					1449:1455	fluorescent and patterned device surface	1416:1455	fluorescent and patterned device surface	1416:1455	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	4	9	theme	deposited	685:693	arg1	surface					695:701	the deposited surface	681:701	the deposited surface	681:701	It is shown that the deposited surface displays clear fluorescence under UV light, and the fluorescence intensity of the surface can be controlled by electrodeposition conditions (e.g., deposition time).
25133925	1	10	theme	controllable	283:294	arg1	means					296:300	a controllable means	281:300	a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications	281:400	Electroaddressing is an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control, and it also offers a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications.
25133925	1	11	theme	applications	389:400	arg1	range					380:384	a diverse range	370:384	a diverse range of applications	370:400	Electroaddressing is an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control, and it also offers a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications.
25133925	4	12	theme	surface	785:791	arg1	intensity					768:776	the fluorescence intensity	751:776	the fluorescence intensity of the surface	751:791	It is shown that the deposited surface displays clear fluorescence under UV light, and the fluorescence intensity of the surface can be controlled by electrodeposition conditions (e.g., deposition time).
25133925	5	13	with	electrodes	947:956	arg1	shapes					985:990	various shapes	977:990	various shapes	977:990	Furthermore, a variety of fluorescent patterns can be constructed by employing electrodes or substrates with various shapes.
25133925	6	14	theme	fluorescent	1144:1154	arg1	patterns					1156:1163	diverse fluorescent patterns	1136:1163	diverse fluorescent patterns	1136:1163	Specifically, taking advantage of the spatiotemporal selectivity of electroaddressing and the pH-responsive property of chitosan, we construct diverse fluorescent patterns by electroaddressing QDs/chitosan composite at the localized region.
25133925	8	15	theme	patterned	1432:1440	arg1	surface					1449:1455	fluorescent and patterned device surface	1416:1455	fluorescent and patterned device surface	1416:1455	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	2	16	theme	novel	426:430	arg1	method					432:437	a novel method	424:437	a novel method to construct fluorescent and patterned device surfaces by electroaddressing of quantum dots (QDs)/chitosan composite	424:554	Here, we demonstrate a novel method to construct fluorescent and patterned device surfaces by electroaddressing of quantum dots (QDs)/chitosan composite.
25133925	0	17	theme	device	107:112	arg1	surface					114:120	fluorescent and patterned device surface	81:120	fluorescent and patterned device surface	81:120	Electroaddressing of ZnS quantum dots by codeposition with chitosan to construct fluorescent and patterned device surface.
25133925	8	18	contain	has	1485:1487	arg2	applications					1499:1510	promising applications	1489:1510	promising applications	1489:1510	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	8	18	contain	has	1485:1487	arg1	electroaddressing					1460:1476	electroaddressing	1460:1476	electroaddressing	1460:1476	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	6	19	theme	localized	1216:1224	arg1	region					1226:1231	the localized region	1212:1231	the localized region	1212:1231	Specifically, taking advantage of the spatiotemporal selectivity of electroaddressing and the pH-responsive property of chitosan, we construct diverse fluorescent patterns by electroaddressing QDs/chitosan composite at the localized region.
25133925	7	20	theme	fluorescent	1260:1270	arg1	patterns					1272:1279	the fluorescent patterns	1256:1279	the fluorescent patterns of QDs/chitosan composite	1256:1305	It is also found that the fluorescent patterns of QDs/chitosan composite have reproducibility.
25133925	3	21	theme	composite	597:605	arg1	surfaces					568:575	the surfaces	564:575	the surfaces of ZnS QDs/chitosan composite on the electrodes	564:623	First, the surfaces of ZnS QDs/chitosan composite on the electrodes are built by electrodeposition method.
25133925	1	22	theme	stimuli-responsive	192:209	arg1	biopolymers					211:221	stimuli-responsive biopolymers	192:221	stimuli-responsive biopolymers with exquisite spatiotemporal control	192:259	Electroaddressing is an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control, and it also offers a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications.
25133925	2	23	theme	device	478:483	arg1	surfaces					485:492	fluorescent and patterned device surfaces	452:492	fluorescent and patterned device surfaces	452:492	Here, we demonstrate a novel method to construct fluorescent and patterned device surfaces by electroaddressing of quantum dots (QDs)/chitosan composite.
25133925	4	24	theme	fluorescence	755:766	arg1	intensity					768:776	the fluorescence intensity	751:776	the fluorescence intensity of the surface	751:791	It is shown that the deposited surface displays clear fluorescence under UV light, and the fluorescence intensity of the surface can be controlled by electrodeposition conditions (e.g., deposition time).
25133925	1	25	theme	biopolymers	211:221	arg1	assembly					180:187	assembly	180:187	assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control	180:259	Electroaddressing is an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control, and it also offers a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications.
25133925	0	26	theme	quantum	25:31	arg1	dots					33:36	ZnS quantum dots	21:36	ZnS quantum dots	21:36	Electroaddressing of ZnS quantum dots by codeposition with chitosan to construct fluorescent and patterned device surface.
25133925	3	27	theme	ZnS	580:582	arg1	composite					597:605	ZnS QDs/chitosan composite	580:605	ZnS QDs/chitosan composite	580:605	First, the surfaces of ZnS QDs/chitosan composite on the electrodes are built by electrodeposition method.
25133925	2	28	theme	fluorescent	452:462	arg1	surfaces					485:492	fluorescent and patterned device surfaces	452:492	fluorescent and patterned device surfaces	452:492	Here, we demonstrate a novel method to construct fluorescent and patterned device surfaces by electroaddressing of quantum dots (QDs)/chitosan composite.
25133925	0	29	theme	ZnS	21:23	arg1	dots					33:36	ZnS quantum dots	21:36	ZnS quantum dots	21:36	Electroaddressing of ZnS quantum dots by codeposition with chitosan to construct fluorescent and patterned device surface.
25133925	8	30	theme	fluorescent	1541:1551	arg1	coating					1567:1573	fluorescent and patterned coating	1541:1573	fluorescent and patterned coating	1541:1573	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	3	31	theme	QDs/chitosan	584:595	arg1	composite					597:605	ZnS QDs/chitosan composite	580:605	ZnS QDs/chitosan composite	580:605	First, the surfaces of ZnS QDs/chitosan composite on the electrodes are built by electrodeposition method.
25133925	1	32	theme	exquisite	228:236	arg1	control					253:259	exquisite spatiotemporal control	228:259	exquisite spatiotemporal control	228:259	Electroaddressing is an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control, and it also offers a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications.
25133925	2	33	theme	quantum	518:524	arg1	QDs					532:534	QDs	532:534	QDs	532:534	Here, we demonstrate a novel method to construct fluorescent and patterned device surfaces by electroaddressing of quantum dots (QDs)/chitosan composite.
25133925	2	33	theme	quantum	518:524	arg1	dots					526:529	quantum dots	518:529	quantum dots (QDs)	518:535	Here, we demonstrate a novel method to construct fluorescent and patterned device surfaces by electroaddressing of quantum dots (QDs)/chitosan composite.
25133925	1	34	theme	biological	327:336	arg1	materials					338:346	biological materials	327:346	biological materials	327:346	Electroaddressing is an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control, and it also offers a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications.
25133925	6	35	theme	pH-responsive	1087:1099	arg1	property					1101:1108	the pH-responsive property	1083:1108	the pH-responsive property of chitosan	1083:1120	Specifically, taking advantage of the spatiotemporal selectivity of electroaddressing and the pH-responsive property of chitosan, we construct diverse fluorescent patterns by electroaddressing QDs/chitosan composite at the localized region.
25133925	8	36	theme	controllable	1383:1394	arg1	method					1396:1401	a convenient, versatile, and controllable method	1354:1401	a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice	1354:1602	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	5	37	theme	fluorescent	894:904	arg1	patterns					906:913	fluorescent patterns	894:913	fluorescent patterns	894:913	Furthermore, a variety of fluorescent patterns can be constructed by employing electrodes or substrates with various shapes.
25133925	1	38	theme	spatiotemporal	238:251	arg1	control					253:259	exquisite spatiotemporal control	228:259	exquisite spatiotemporal control	228:259	Electroaddressing is an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control, and it also offers a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications.
25133925	0	39	theme	dots	33:36	arg1	Electroaddressing					0:16	Electroaddressing	0:16	Electroaddressing of ZnS quantum dots by codeposition with chitosan	0:66	Electroaddressing of ZnS quantum dots by codeposition with chitosan to construct fluorescent and patterned device surface.
25133925	5	40	with	substrates	961:970	arg1	shapes					985:990	various shapes	977:990	various shapes	977:990	Furthermore, a variety of fluorescent patterns can be constructed by employing electrodes or substrates with various shapes.
25133925	8	41	theme	patterned	1557:1565	arg1	coating					1567:1573	fluorescent and patterned coating	1541:1573	fluorescent and patterned coating	1541:1573	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	2	42	theme	patterned	468:476	arg1	surfaces					485:492	fluorescent and patterned device surfaces	452:492	fluorescent and patterned device surfaces	452:492	Here, we demonstrate a novel method to construct fluorescent and patterned device surfaces by electroaddressing of quantum dots (QDs)/chitosan composite.
25133925	5	43	theme	patterns	906:913	arg1	variety					883:889	a variety	881:889	a variety of fluorescent patterns	881:913	Furthermore, a variety of fluorescent patterns can be constructed by employing electrodes or substrates with various shapes.
25133925	5	43	theme	patterns	906:913	arg1	patterns					906:913	fluorescent patterns	894:913	fluorescent patterns	894:913	Furthermore, a variety of fluorescent patterns can be constructed by employing electrodes or substrates with various shapes.
25133925	8	44	theme	promising	1489:1497	arg1	applications					1499:1510	promising applications	1489:1510	promising applications	1489:1510	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	8	45	theme	device	1442:1447	arg1	surface					1449:1455	fluorescent and patterned device surface	1416:1455	fluorescent and patterned device surface	1416:1455	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	1	46	with	biopolymers	211:221	arg1	control					253:259	exquisite spatiotemporal control	228:259	exquisite spatiotemporal control	228:259	Electroaddressing is an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control, and it also offers a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications.
25133925	4	47	theme	deposition	850:859	arg1	time					861:864	deposition time	850:864	deposition time	850:864	It is shown that the deposited surface displays clear fluorescence under UV light, and the fluorescence intensity of the surface can be controlled by electrodeposition conditions (e.g., deposition time).
25133925	8	48	theme	convenient	1356:1365	arg1	method					1396:1401	a convenient, versatile, and controllable method	1354:1401	a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice	1354:1602	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	8	49	theme	photoluminescence	1515:1531	arg1	device					1533:1538	photoluminescence device	1515:1538	photoluminescence device	1515:1538	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	4	50	theme	electrodeposition	814:830	arg1	conditions					832:841	electrodeposition conditions	814:841	electrodeposition conditions (e.g., deposition time)	814:865	It is shown that the deposited surface displays clear fluorescence under UV light, and the fluorescence intensity of the surface can be controlled by electrodeposition conditions (e.g., deposition time).
25133925	0	51	with	Electroaddressing	0:16	arg1	chitosan					59:66	chitosan	59:66	chitosan	59:66	Electroaddressing of ZnS quantum dots by codeposition with chitosan to construct fluorescent and patterned device surface.
25133925	6	52	theme	QDs/chitosan	1186:1197	arg1	composite					1199:1207	QDs/chitosan composite	1186:1207	QDs/chitosan composite	1186:1207	Specifically, taking advantage of the spatiotemporal selectivity of electroaddressing and the pH-responsive property of chitosan, we construct diverse fluorescent patterns by electroaddressing QDs/chitosan composite at the localized region.
25133925	5	53	theme	various	977:983	arg1	shapes					985:990	various shapes	977:990	various shapes	977:990	Furthermore, a variety of fluorescent patterns can be constructed by employing electrodes or substrates with various shapes.
25133925	8	54	theme	nanocomposite	1580:1592	arg1	biodevice					1594:1602	nanocomposite biodevice	1580:1602	nanocomposite biodevice	1580:1602	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	6	55	theme	chitosan	1113:1120	arg1	property					1101:1108	the pH-responsive property	1083:1108	the pH-responsive property of chitosan	1083:1120	Specifically, taking advantage of the spatiotemporal selectivity of electroaddressing and the pH-responsive property of chitosan, we construct diverse fluorescent patterns by electroaddressing QDs/chitosan composite at the localized region.
25133925	6	55	theme	chitosan	1113:1120	arg1	advantage					1014:1022	advantage	1014:1022	advantage of the spatiotemporal selectivity of electroaddressing	1014:1077	Specifically, taking advantage of the spatiotemporal selectivity of electroaddressing and the pH-responsive property of chitosan, we construct diverse fluorescent patterns by electroaddressing QDs/chitosan composite at the localized region.
25133925	8	56	theme	versatile	1368:1376	arg1	method					1396:1401	a convenient, versatile, and controllable method	1354:1401	a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice	1354:1602	Thus, this work presents a convenient, versatile, and controllable method to construct fluorescent and patterned device surface by electroaddressing, which has promising applications in photoluminescence device, fluorescent and patterned coating, and nanocomposite biodevice.
25133925	6	57	theme	electroaddressing	1061:1077	arg1	selectivity					1046:1056	the spatiotemporal selectivity	1027:1056	the spatiotemporal selectivity of electroaddressing	1027:1077	Specifically, taking advantage of the spatiotemporal selectivity of electroaddressing and the pH-responsive property of chitosan, we construct diverse fluorescent patterns by electroaddressing QDs/chitosan composite at the localized region.
25133925	7	58	theme	composite	1297:1305	arg1	patterns					1272:1279	the fluorescent patterns	1256:1279	the fluorescent patterns of QDs/chitosan composite	1256:1305	It is also found that the fluorescent patterns of QDs/chitosan composite have reproducibility.
25133925	4	59	dep	time	861:864	arg1	e.g.					844:847	e.g.	844:847	e.g.	844:847	It is shown that the deposited surface displays clear fluorescence under UV light, and the fluorescence intensity of the surface can be controlled by electrodeposition conditions (e.g., deposition time).
25133925	1	60	theme	attractive	147:156	arg1	method					158:163	an attractive method	144:163	an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control	144:259	Electroaddressing is an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control, and it also offers a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications.
25133925	1	60	theme	attractive	147:156	arg1	Electroaddressing					123:139	Electroaddressing	123:139	Electroaddressing	123:139	Electroaddressing is an attractive method for triggering assembly of stimuli-responsive biopolymers with exquisite spatiotemporal control, and it also offers a controllable means to concurrently assemble biological materials and nanoparticles for a diverse range of applications.
25133925	3	61	theme	electrodeposition	638:654	arg1	method					656:661	electrodeposition method	638:661	electrodeposition method	638:661	First, the surfaces of ZnS QDs/chitosan composite on the electrodes are built by electrodeposition method.
25133925	7	62	contain	have	1307:1310	arg2	reproducibility					1312:1326	reproducibility	1312:1326	reproducibility	1312:1326	It is also found that the fluorescent patterns of QDs/chitosan composite have reproducibility.
25133925	7	62	contain	have	1307:1310	arg1	patterns					1272:1279	the fluorescent patterns	1256:1279	the fluorescent patterns of QDs/chitosan composite	1256:1305	It is also found that the fluorescent patterns of QDs/chitosan composite have reproducibility.
25133925	4	63	theme	clear	712:716	arg1	fluorescence					718:729	clear fluorescence	712:729	clear fluorescence	712:729	It is shown that the deposited surface displays clear fluorescence under UV light, and the fluorescence intensity of the surface can be controlled by electrodeposition conditions (e.g., deposition time).
25133925	6	64	theme	selectivity	1046:1056	arg1	property					1101:1108	the pH-responsive property	1083:1108	the pH-responsive property of chitosan	1083:1120	Specifically, taking advantage of the spatiotemporal selectivity of electroaddressing and the pH-responsive property of chitosan, we construct diverse fluorescent patterns by electroaddressing QDs/chitosan composite at the localized region.
25133925	6	64	theme	selectivity	1046:1056	arg1	advantage					1014:1022	advantage	1014:1022	advantage of the spatiotemporal selectivity of electroaddressing	1014:1077	Specifically, taking advantage of the spatiotemporal selectivity of electroaddressing and the pH-responsive property of chitosan, we construct diverse fluorescent patterns by electroaddressing QDs/chitosan composite at the localized region.
26249848	1	0	theme	UV	171:172	arg1	detection					174:182	an indirect UV detection	159:182	an indirect UV detection	159:182	The CE method employing an indirect UV detection for the enantioseparation of 1,3-dimethylamylamine (DMAA), widely used in various preworkout and dietary supplements labeled as a constituent of geranium extract has been developed.
26249848	3	1	theme	dietary	851:857	arg1	supplements					859:869	commercial dietary supplements	840:869	commercial dietary supplements	840:869	The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
26249848	1	2	theme	various	258:264	arg1	preworkout					266:275	various preworkout	258:275	various preworkout	258:275	The CE method employing an indirect UV detection for the enantioseparation of 1,3-dimethylamylamine (DMAA), widely used in various preworkout and dietary supplements labeled as a constituent of geranium extract has been developed.
26249848	2	3	from	LODs	691:694	arg1	range					703:707	the range	699:707	the range of 7.82-9.24 μg/mL	699:726	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	4	theme	chloride	573:580	arg1	addition					532:539	the addition	528:539	the addition of 10 mM benzyltriethylammonium chloride (BTEAC)	528:588	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	4	theme	chloride	573:580	arg1	additive					610:617	additive	610:617	additive	610:617	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	5	used	used	623:626	arg2	system					384:389	The dual-selector system	366:389	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive	366:617	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	3	6	theme	DMAA	832:835	arg1	composition					817:827	the stereoisomeric composition	798:827	the stereoisomeric composition of DMAA in commercial dietary supplements	798:869	The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
26249848	0	7	theme	dual-selector	113:125	arg1	system					127:132	a dual-selector system	111:132	a dual-selector system	111:132	Enantiomeric separation of 1,3-dimethylamylamine by capillary electrophoresis with indirect UV detection using a dual-selector system.
26249848	2	8	theme	enantiomeric	636:647	arg1	separation					649:658	the enantiomeric separation	632:658	the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL	632:726	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	9	theme	benzyltriethylammonium	550:571	arg1	chloride					573:580	10 mM benzyltriethylammonium chloride	544:580	10 mM benzyltriethylammonium chloride (BTEAC)	544:588	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	9	theme	benzyltriethylammonium	550:571	arg1	BTEAC					583:587	BTEAC	583:587	BTEAC	583:587	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	10	theme	mM	547:548	arg1	chloride					573:580	10 mM benzyltriethylammonium chloride	544:580	10 mM benzyltriethylammonium chloride (BTEAC)	544:588	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	10	theme	mM	547:548	arg1	BTEAC					583:587	BTEAC	583:587	BTEAC	583:587	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	11	theme	dual-selector	370:382	arg1	system					384:389	The dual-selector system	366:389	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive	366:617	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	12	theme	μg/mL	722:726	arg1	range					703:707	the range	699:707	the range of 7.82-9.24 μg/mL	699:726	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	1	13	theme	dietary	281:287	arg1	supplements					289:299	dietary supplements	281:299	dietary supplements	281:299	The CE method employing an indirect UV detection for the enantioseparation of 1,3-dimethylamylamine (DMAA), widely used in various preworkout and dietary supplements labeled as a constituent of geranium extract has been developed.
26249848	2	14	theme	phosphate/Tris	486:499	arg1	pH					509:510	pH 3.0	509:514	pH 3.0	509:514	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	14	theme	phosphate/Tris	486:499	arg1	buffer					501:506	5 mM phosphate/Tris buffer	481:506	5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive	481:617	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	15	from	β-CD	462:465	arg1	pH					509:510	pH 3.0	509:514	pH 3.0	509:514	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	15	from	β-CD	462:465	arg1	buffer					501:506	5 mM phosphate/Tris buffer	481:506	5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive	481:617	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	16	theme	sulfated	424:431	arg1	w/v					444:446	1.1% w/v	439:446	1.1% w/v	439:446	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	16	theme	sulfated	424:431	arg1	α-CD					433:436	negatively charged sulfated α-CD	405:436	negatively charged sulfated α-CD (1.1% w/v)	405:447	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	17	theme	mM	483:484	arg1	pH					509:510	pH 3.0	509:514	pH 3.0	509:514	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	17	theme	mM	483:484	arg1	buffer					501:506	5 mM phosphate/Tris buffer	481:506	5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive	481:617	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	18	theme	%	471:471	arg1	w/v					473:475	0.2% w/v	468:475	0.2% w/v	468:475	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	18	theme	%	471:471	arg1	β-CD					462:465	sulfated β-CD	453:465	sulfated β-CD (0.2% w/v)	453:476	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	19	theme	charged	416:422	arg1	w/v					444:446	1.1% w/v	439:446	1.1% w/v	439:446	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	19	theme	charged	416:422	arg1	α-CD					433:436	negatively charged sulfated α-CD	405:436	negatively charged sulfated α-CD (1.1% w/v)	405:447	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	20	theme	sulfated	453:460	arg1	w/v					473:475	0.2% w/v	468:475	0.2% w/v	468:475	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	20	theme	sulfated	453:460	arg1	β-CD					462:465	sulfated β-CD	453:465	sulfated β-CD (0.2% w/v)	453:476	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	0	21	theme	UV	92:93	arg1	detection					95:103	indirect UV detection	83:103	indirect UV detection using a dual-selector system	83:132	Enantiomeric separation of 1,3-dimethylamylamine by capillary electrophoresis with indirect UV detection using a dual-selector system.
26249848	2	22	contain	containing	517:526	arg2	addition					532:539	the addition	528:539	the addition of 10 mM benzyltriethylammonium chloride (BTEAC)	528:588	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	22	contain	containing	517:526	arg1	buffer					501:506	5 mM phosphate/Tris buffer	481:506	5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive	481:617	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	22	contain	containing	517:526	arg1	pH					509:510	pH 3.0	509:514	pH 3.0	509:514	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	22	contain	containing	517:526	arg2	additive					610:617	additive	610:617	additive	610:617	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	0	23	theme	Enantiomeric	0:11	arg1	separation					13:22	Enantiomeric separation	0:22	Enantiomeric separation of 1,3-dimethylamylamine by capillary electrophoresis with indirect UV detection using a dual-selector system.	0:133	Enantiomeric separation of 1,3-dimethylamylamine by capillary electrophoresis with indirect UV detection using a dual-selector system.
26249848	0	24	theme	1,3-dimethylamylamine	27:47	arg1	separation					13:22	Enantiomeric separation	0:22	Enantiomeric separation of 1,3-dimethylamylamine by capillary electrophoresis with indirect UV detection using a dual-selector system.	0:133	Enantiomeric separation of 1,3-dimethylamylamine by capillary electrophoresis with indirect UV detection using a dual-selector system.
26249848	3	25	from	composition	817:827	arg1	supplements					859:869	commercial dietary supplements	840:869	commercial dietary supplements	840:869	The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
26249848	2	26	theme	%	442:442	arg1	w/v					444:446	1.1% w/v	439:446	1.1% w/v	439:446	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	26	theme	%	442:442	arg1	α-CD					433:436	negatively charged sulfated α-CD	405:436	negatively charged sulfated α-CD (1.1% w/v)	405:447	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	27	from	α-CD	433:436	arg1	pH					509:510	pH 3.0	509:514	pH 3.0	509:514	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	27	from	α-CD	433:436	arg1	buffer					501:506	5 mM phosphate/Tris buffer	481:506	5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive	481:617	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	3	28	theme	DMAA	913:916	arg1	origin					903:908	the potential natural origin	881:908	the potential natural origin of DMAA	881:916	The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
26249848	3	29	theme	potential	885:893	arg1	origin					903:908	the potential natural origin	881:908	the potential natural origin of DMAA	881:916	The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
26249848	0	30	theme	capillary	52:60	arg1	electrophoresis					62:76	capillary electrophoresis	52:76	capillary electrophoresis with indirect UV detection using a dual-selector system	52:132	Enantiomeric separation of 1,3-dimethylamylamine by capillary electrophoresis with indirect UV detection using a dual-selector system.
26249848	3	31	from	determination	781:793	arg1	supplements					859:869	commercial dietary supplements	840:869	commercial dietary supplements	840:869	The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
26249848	3	32	theme	natural	895:901	arg1	origin					903:908	the potential natural origin	881:908	the potential natural origin of DMAA	881:916	The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
26249848	2	33	theme	10	544:545	arg1	mM					547:548	mM	547:548	mM	547:548	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	2	34	theme	stereoisomers	668:680	arg1	separation					649:658	the enantiomeric separation	632:658	the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL	632:726	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	1	35	theme	geranium	329:336	arg1	extract					338:344	geranium extract	329:344	geranium extract	329:344	The CE method employing an indirect UV detection for the enantioseparation of 1,3-dimethylamylamine (DMAA), widely used in various preworkout and dietary supplements labeled as a constituent of geranium extract has been developed.
26249848	2	36	theme	DMAA	663:666	arg1	stereoisomers					668:680	DMAA stereoisomers	663:680	DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL	663:726	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	1	37	theme	CE	139:140	arg1	method					142:147	The CE method	135:147	The CE method employing an indirect UV detection for the enantioseparation of 1,3-dimethylamylamine (DMAA), widely used in various preworkout and dietary supplements labeled as a constituent of geranium extract	135:344	The CE method employing an indirect UV detection for the enantioseparation of 1,3-dimethylamylamine (DMAA), widely used in various preworkout and dietary supplements labeled as a constituent of geranium extract has been developed.
26249848	3	38	theme	composition	817:827	arg1	determination					781:793	the determination	777:793	the determination of the stereoisomeric composition of DMAA in commercial dietary supplements	777:869	The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
26249848	1	39	theme	extract	338:344	arg1	constituent					314:324	a constituent	312:324	a constituent of geranium extract	312:344	The CE method employing an indirect UV detection for the enantioseparation of 1,3-dimethylamylamine (DMAA), widely used in various preworkout and dietary supplements labeled as a constituent of geranium extract has been developed.
26249848	3	40	from	supplements	859:869	arg1	determination					781:793	the determination	777:793	the determination of the stereoisomeric composition of DMAA in commercial dietary supplements	777:869	The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
26249848	2	41	theme	5	481:481	arg1	mM					483:484	mM	483:484	mM	483:484	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	1	42	theme	1,3-dimethylamylamine	213:233	arg1	enantioseparation					192:208	the enantioseparation	188:208	the enantioseparation	188:208	The CE method employing an indirect UV detection for the enantioseparation of 1,3-dimethylamylamine (DMAA), widely used in various preworkout and dietary supplements labeled as a constituent of geranium extract has been developed.
26249848	0	43	with	electrophoresis	62:76	arg1	detection					95:103	indirect UV detection	83:103	indirect UV detection using a dual-selector system	83:132	Enantiomeric separation of 1,3-dimethylamylamine by capillary electrophoresis with indirect UV detection using a dual-selector system.
26249848	3	44	theme	stereoisomeric	802:815	arg1	composition					817:827	the stereoisomeric composition	798:827	the stereoisomeric composition of DMAA in commercial dietary supplements	798:869	The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
26249848	0	45	theme	indirect	83:90	arg1	detection					95:103	indirect UV detection	83:103	indirect UV detection using a dual-selector system	83:132	Enantiomeric separation of 1,3-dimethylamylamine by capillary electrophoresis with indirect UV detection using a dual-selector system.
26249848	2	46	with	stereoisomers	668:680	arg1	LODs					691:694	the LODs	687:694	the LODs in the range of 7.82-9.24 μg/mL	687:726	The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL.
26249848	1	47	theme	indirect	162:169	arg1	detection					174:182	an indirect UV detection	159:182	an indirect UV detection	159:182	The CE method employing an indirect UV detection for the enantioseparation of 1,3-dimethylamylamine (DMAA), widely used in various preworkout and dietary supplements labeled as a constituent of geranium extract has been developed.
26249848	3	48	theme	commercial	840:849	arg1	supplements					859:869	commercial dietary supplements	840:869	commercial dietary supplements	840:869	The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
25816898	0	0	theme	sugars	64:69	arg1	acids					72:76	acids	72:76	acids	72:76	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	0	theme	sugars	64:69	arg1	sugars					64:69	sugars	64:69	sugars	64:69	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	0	theme	sugars	64:69	arg1	measurements					115:126	physicochemical measurements	99:126	physicochemical measurements	99:126	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	0	theme	sugars	64:69	arg1	amount					54:59	amount	54:59	amount of sugars, acids, anthocyanidins, and physicochemical measurements	54:126	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	0	theme	sugars	64:69	arg1	anthocyanidins					79:92	anthocyanidins	79:92	anthocyanidins	79:92	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	14	1	theme	BRIX	1530:1533	arg1	ratio					1538:1542	BRIX:TA ratio	1530:1542	BRIX:TA ratio	1530:1542	The information in this component was related to pH, TA, and BRIX:TA ratio.
25816898	17	2	theme	juice	1707:1711	arg1	composition					1713:1723	juice composition	1707:1723	juice composition	1707:1723	This work shows that the impact of juice composition on flavor is very complicated and that estimating flavor with physicochemical parameters is complicated by the composition of the juice.
25816898	15	3	theme	nonblueberry	1585:1596	arg1	flavors					1604:1610	the nonblueberry fruit flavors	1581:1610	the nonblueberry fruit flavors	1581:1610	Another principal component related the nonblueberry fruit flavors to BRIX.
25816898	4	4	theme	titratable	521:530	arg1	TA					541:542	TA	541:542	TA	541:542	Each sample was measured for sugars, acids, anthocyanidins, Folin-Ciocalteu, soluble solids (BRIX), titratable acidity (TA), and antioxidant capacity (ORACFL ).
25816898	4	4	theme	titratable	521:530	arg1	acidity					532:538	titratable acidity	521:538	titratable acidity (TA)	521:543	Each sample was measured for sugars, acids, anthocyanidins, Folin-Ciocalteu, soluble solids (BRIX), titratable acidity (TA), and antioxidant capacity (ORACFL ).
25816898	0	5	theme	acids	72:76	arg1	acids					72:76	acids	72:76	acids	72:76	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	5	theme	acids	72:76	arg1	sugars					64:69	sugars	64:69	sugars	64:69	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	5	theme	acids	72:76	arg1	measurements					115:126	physicochemical measurements	99:126	physicochemical measurements	99:126	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	5	theme	acids	72:76	arg1	amount					54:59	amount	54:59	amount of sugars, acids, anthocyanidins, and physicochemical measurements	54:126	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	5	theme	acids	72:76	arg1	anthocyanidins					79:92	anthocyanidins	79:92	anthocyanidins	79:92	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	17	6	theme	composition	1713:1723	arg1	complicated					1743:1753	complicated	1743:1753	complicated	1743:1753	This work shows that the impact of juice composition on flavor is very complicated and that estimating flavor with physicochemical parameters is complicated by the composition of the juice.
25816898	17	6	theme	composition	1713:1723	arg1	impact					1697:1702	the impact	1693:1702	the impact of juice composition on flavor	1693:1733	This work shows that the impact of juice composition on flavor is very complicated and that estimating flavor with physicochemical parameters is complicated by the composition of the juice.
25816898	12	7	theme	total	1302:1306	arg1	acids					1308:1312	total acids	1302:1312	total acids	1302:1312	Throat burn correlated with total acids and TA.
25816898	15	8	theme	principal	1553:1561	arg1	component					1563:1571	Another principal component	1545:1571	Another principal component	1545:1571	Another principal component related the nonblueberry fruit flavors to BRIX.
25816898	6	9	theme	Blueberry	653:661	arg1	flavor					663:668	Blueberry flavor	653:668	Blueberry flavor	653:668	Blueberry flavor correlated with 3 parameters, and negatively correlated with 2.
25816898	0	10	theme	anthocyanidins	79:92	arg1	acids					72:76	acids	72:76	acids	72:76	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	10	theme	anthocyanidins	79:92	arg1	sugars					64:69	sugars	64:69	sugars	64:69	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	10	theme	anthocyanidins	79:92	arg1	measurements					115:126	physicochemical measurements	99:126	physicochemical measurements	99:126	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	10	theme	anthocyanidins	79:92	arg1	amount					54:59	amount	54:59	amount of sugars, acids, anthocyanidins, and physicochemical measurements	54:126	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	10	theme	anthocyanidins	79:92	arg1	anthocyanidins					79:92	anthocyanidins	79:92	anthocyanidins	79:92	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	16	11	from	RE	1668:1669	arg1	berries					1651:1657	the SHB berries	1643:1657	the SHB berries from the RE	1643:1669	This PC, also divided the SHB berries from the RE.
25816898	7	12	theme	quinic	816:821	arg1	acid					823:826	quinic acid	816:826	quinic acid	816:826	Strawberry correlated with oxalic acid and negatively correlated with sucrose and quinic acid.
25816898	1	13	theme	southern	146:153	arg1	SHB					165:167	SHB	165:167	SHB	165:167	Six cultivars of southern highbush (SHB) and rabbiteye (RE) blueberry samples were harvested on 2 different dates.
25816898	1	13	theme	southern	146:153	arg1	highbush					155:162	southern highbush	146:162	southern highbush (SHB)	146:168	Six cultivars of southern highbush (SHB) and rabbiteye (RE) blueberry samples were harvested on 2 different dates.
25816898	9	14	theme	Sweet	946:950	arg1	taste					952:956	Sweet taste	946:956	Sweet taste	946:956	Sweet taste correlated with 11 parameters, including the anthocyanidins; and negatively correlated with 3 parameters.
25816898	17	15	theme	juice	1855:1859	arg1	composition					1836:1846	the composition	1832:1846	the composition of the juice	1832:1859	This work shows that the impact of juice composition on flavor is very complicated and that estimating flavor with physicochemical parameters is complicated by the composition of the juice.
25816898	17	16	from	impact	1697:1702	arg1	flavor					1728:1733	flavor	1728:1733	flavor	1728:1733	This work shows that the impact of juice composition on flavor is very complicated and that estimating flavor with physicochemical parameters is complicated by the composition of the juice.
25816898	1	17	theme	highbush	155:162	arg1	cultivars					133:141	Six cultivars	129:141	Six cultivars of southern highbush (SHB) and rabbiteye (RE) blueberry samples	129:205	Six cultivars of southern highbush (SHB) and rabbiteye (RE) blueberry samples were harvested on 2 different dates.
25816898	8	18	theme	total	933:937	arg1	acids					939:943	total acids	933:943	total acids	933:943	Sweet aroma correlated with oxalic and citric acid, but negatively correlated with sucrose, quinic, and total acids.
25816898	8	19	theme	Sweet	829:833	arg1	aroma					835:839	Sweet aroma	829:839	Sweet aroma	829:839	Sweet aroma correlated with oxalic and citric acid, but negatively correlated with sucrose, quinic, and total acids.
25816898	1	20	theme	different	227:235	arg1	dates					237:241	2 different dates	225:241	2 different dates	225:241	Six cultivars of southern highbush (SHB) and rabbiteye (RE) blueberry samples were harvested on 2 different dates.
25816898	17	21	with	flavor	1775:1780	arg1	parameters					1803:1812	physicochemical parameters	1787:1812	physicochemical parameters	1787:1812	This work shows that the impact of juice composition on flavor is very complicated and that estimating flavor with physicochemical parameters is complicated by the composition of the juice.
25816898	3	22	theme	descriptive	395:405	arg1	panel					414:418	a descriptive flavor panel	393:418	a descriptive flavor panel	393:418	Fresh juice was characterized for 18 flavor/taste/feeling factor attributes by a descriptive flavor panel.
25816898	0	23	theme	blueberry	16:24	arg1	juice					26:30	fresh blueberry juice	10:30	fresh blueberry juice	10:30	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	7	24	theme	oxalic	761:766	arg1	acid					768:771	oxalic acid	761:771	oxalic acid	761:771	Strawberry correlated with oxalic acid and negatively correlated with sucrose and quinic acid.
25816898	5	25	theme	physicochemical	631:645	arg1	data					647:650	the composition and physicochemical data	611:650	data	647:650	Flavors were correlated with the composition and physicochemical data.
25816898	12	26	theme	Throat	1274:1279	arg1	burn					1281:1284	Throat burn	1274:1284	Throat burn	1274:1284	Throat burn correlated with total acids and TA.
25816898	0	27	theme	fresh	10:14	arg1	juice					26:30	fresh blueberry juice	10:30	fresh blueberry juice	10:30	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	2	28	theme	treatment	249:257	arg1	combination					259:269	Each treatment combination	244:269	Each treatment combination	244:269	Each treatment combination was pressed 2 times for repeated measures.
25816898	15	29	theme	fruit	1598:1602	arg1	flavors					1604:1610	the nonblueberry fruit flavors	1581:1610	the nonblueberry fruit flavors	1581:1610	Another principal component related the nonblueberry fruit flavors to BRIX.
25816898	17	30	theme	physicochemical	1787:1801	arg1	parameters					1803:1812	physicochemical parameters	1787:1812	physicochemical parameters	1787:1812	This work shows that the impact of juice composition on flavor is very complicated and that estimating flavor with physicochemical parameters is complicated by the composition of the juice.
25816898	13	31	theme	Principal	1322:1330	arg1	analysis					1342:1349	Principal component analysis	1322:1349	Principal component analysis	1322:1349	Principal component analysis negatively related blueberry, sweet aroma, and sweet to sour, bitter, astringent, tongue tingle, and tongue numbness.
25816898	0	32	theme	measurements	115:126	arg1	acids					72:76	acids	72:76	acids	72:76	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	32	theme	measurements	115:126	arg1	sugars					64:69	sugars	64:69	sugars	64:69	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	32	theme	measurements	115:126	arg1	measurements					115:126	physicochemical measurements	99:126	physicochemical measurements	99:126	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	32	theme	measurements	115:126	arg1	amount					54:59	amount	54:59	amount of sugars, acids, anthocyanidins, and physicochemical measurements	54:126	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	32	theme	measurements	115:126	arg1	anthocyanidins					79:92	anthocyanidins	79:92	anthocyanidins	79:92	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	8	33	theme	oxalic	857:862	arg1	acid					875:878	oxalic and citric acid	857:878	acid	875:878	Sweet aroma correlated with oxalic and citric acid, but negatively correlated with sucrose, quinic, and total acids.
25816898	1	34	theme	rabbiteye	174:182	arg1	cultivars					133:141	Six cultivars	129:141	Six cultivars of southern highbush (SHB) and rabbiteye (RE) blueberry samples	129:205	Six cultivars of southern highbush (SHB) and rabbiteye (RE) blueberry samples were harvested on 2 different dates.
25816898	0	35	theme	juice	26:30	arg1	Flavor					0:5	Flavor	0:5	Flavor of fresh blueberry juice	0:30	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	35	theme	juice	26:30	arg1	comparison					40:49	the comparison	36:49	the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements	36:126	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	0	36	theme	physicochemical	99:113	arg1	measurements					115:126	physicochemical measurements	99:126	physicochemical measurements	99:126	Flavor of fresh blueberry juice and the comparison to amount of sugars, acids, anthocyanidins, and physicochemical measurements.
25816898	4	37	theme	antioxidant	550:560	arg1	ORACFL					572:577	ORACFL	572:577	ORACFL	572:577	Each sample was measured for sugars, acids, anthocyanidins, Folin-Ciocalteu, soluble solids (BRIX), titratable acidity (TA), and antioxidant capacity (ORACFL ).
25816898	4	37	theme	antioxidant	550:560	arg1	capacity					562:569	antioxidant capacity	550:569	antioxidant capacity (ORACFL )	550:579	Each sample was measured for sugars, acids, anthocyanidins, Folin-Ciocalteu, soluble solids (BRIX), titratable acidity (TA), and antioxidant capacity (ORACFL ).
25816898	3	38	theme	flavor/taste/feeling	351:370	arg1	attributes					379:388	18 flavor/taste/feeling factor attributes	348:388	18 flavor/taste/feeling factor attributes	348:388	Fresh juice was characterized for 18 flavor/taste/feeling factor attributes by a descriptive flavor panel.
25816898	3	39	theme	Fresh	314:318	arg1	juice					320:324	Fresh juice	314:324	Fresh juice	314:324	Fresh juice was characterized for 18 flavor/taste/feeling factor attributes by a descriptive flavor panel.
25816898	3	40	theme	flavor	407:412	arg1	panel					414:418	a descriptive flavor panel	393:418	a descriptive flavor panel	393:418	Fresh juice was characterized for 18 flavor/taste/feeling factor attributes by a descriptive flavor panel.
25816898	13	41	theme	tongue	1452:1457	arg1	numbness					1459:1466	tongue numbness	1452:1466	tongue numbness	1452:1466	Principal component analysis negatively related blueberry, sweet aroma, and sweet to sour, bitter, astringent, tongue tingle, and tongue numbness.
25816898	8	42	theme	citric	868:873	arg1	acid					875:878	oxalic and citric acid	857:878	acid	875:878	Sweet aroma correlated with oxalic and citric acid, but negatively correlated with sucrose, quinic, and total acids.
25816898	2	43	theme	repeated	295:302	arg1	measures					304:311	repeated measures	295:311	repeated measures	295:311	Each treatment combination was pressed 2 times for repeated measures.
25816898	3	44	theme	factor	372:377	arg1	attributes					379:388	18 flavor/taste/feeling factor attributes	348:388	18 flavor/taste/feeling factor attributes	348:388	Fresh juice was characterized for 18 flavor/taste/feeling factor attributes by a descriptive flavor panel.
25816898	11	45	theme	total	1204:1208	arg1	acids					1210:1214	total acids	1204:1214	total acids	1204:1214	Sour correlated with total acids and TA, while negatively correlating with pH and BRIX:TA.
25816898	1	46	dep	highbush	155:162	arg1	samples					199:205	blueberry samples	189:205	blueberry samples	189:205	Six cultivars of southern highbush (SHB) and rabbiteye (RE) blueberry samples were harvested on 2 different dates.
25816898	4	47	theme	soluble	498:504	arg1	BRIX					514:517	BRIX	514:517	BRIX	514:517	Each sample was measured for sugars, acids, anthocyanidins, Folin-Ciocalteu, soluble solids (BRIX), titratable acidity (TA), and antioxidant capacity (ORACFL ).
25816898	4	47	theme	soluble	498:504	arg1	solids					506:511	soluble solids	498:511	soluble solids (BRIX)	498:518	Each sample was measured for sugars, acids, anthocyanidins, Folin-Ciocalteu, soluble solids (BRIX), titratable acidity (TA), and antioxidant capacity (ORACFL ).
25816898	5	48	theme	composition	615:625	arg1	data					647:650	the composition and physicochemical data	611:650	data	647:650	Flavors were correlated with the composition and physicochemical data.
25816898	13	49	theme	sweet	1381:1385	arg1	aroma					1387:1391	sweet aroma	1381:1391	sweet aroma	1381:1391	Principal component analysis negatively related blueberry, sweet aroma, and sweet to sour, bitter, astringent, tongue tingle, and tongue numbness.
25816898	10	50	theme	total	1170:1174	arg1	acids					1176:1180	total acids	1170:1180	total acids	1170:1180	Neither bitter nor astringent correlated with any of the antioxidant parameters, but both correlated with total acids.
25816898	10	51	theme	antioxidant	1121:1131	arg1	parameters					1133:1142	the antioxidant parameters	1117:1142	the antioxidant parameters	1117:1142	Neither bitter nor astringent correlated with any of the antioxidant parameters, but both correlated with total acids.
25816898	14	52	theme	TA	1535:1536	arg1	ratio					1538:1542	BRIX:TA ratio	1530:1542	BRIX:TA ratio	1530:1542	The information in this component was related to pH, TA, and BRIX:TA ratio.
25816898	13	53	theme	tongue	1433:1438	arg1	tingle					1440:1445	tongue tingle	1433:1445	tongue tingle	1433:1445	Principal component analysis negatively related blueberry, sweet aroma, and sweet to sour, bitter, astringent, tongue tingle, and tongue numbness.
25816898	13	54	theme	component	1332:1340	arg1	analysis					1342:1349	Principal component analysis	1322:1349	Principal component analysis	1322:1349	Principal component analysis negatively related blueberry, sweet aroma, and sweet to sour, bitter, astringent, tongue tingle, and tongue numbness.
25816898	17	55	theme	estimating	1764:1773	arg1	flavor					1775:1780	estimating flavor	1764:1780	estimating flavor with physicochemical parameters	1764:1812	This work shows that the impact of juice composition on flavor is very complicated and that estimating flavor with physicochemical parameters is complicated by the composition of the juice.
25816898	1	56	theme	blueberry	189:197	arg1	samples					199:205	blueberry samples	189:205	blueberry samples	189:205	Six cultivars of southern highbush (SHB) and rabbiteye (RE) blueberry samples were harvested on 2 different dates.
25816898	14	57	from	information	1473:1483	arg1	component					1493:1501	this component	1488:1501	this component	1488:1501	The information in this component was related to pH, TA, and BRIX:TA ratio.
25816898	16	58	theme	SHB	1647:1649	arg1	berries					1651:1657	the SHB berries	1643:1657	the SHB berries from the RE	1643:1669	This PC, also divided the SHB berries from the RE.
27646631	0	0	theme	mechanical	79:88	arg1	properties					90:99	improved mechanical properties	70:99	improved mechanical properties	70:99	Poly (L-lactic acid) porous scaffold-supported alginate hydrogel with improved mechanical properties and biocompatibility.
27646631	3	1	theme	predefined	661:670	arg1	shape					672:676	predefined shape	661:676	predefined shape	661:676	The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape.
27646631	5	2	from	behavior	787:794	arg1	scaffold					803:810	the scaffold	799:810	the scaffold	799:810	The chondrocyte behavior in the scaffold was determined by inverted microscopy, scanning electron microscopy (SEM) and MTT viability assay.
27646631	9	3	theme	new	1548:1550	arg1	formation					1559:1567	new tissue formation	1548:1567	new tissue formation	1548:1567	The histological analyses suggested that the composite scaffold substantially promotes its integration in the host tissue accompanied with a low inflammatory reaction and new tissue formation.
27646631	2	4	theme	alginate	481:488	arg1	Alg					500:502	Alg	500:502	Alg	500:502	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	2	4	theme	alginate	481:488	arg1	hydrogel					490:497	the alginate hydrogel	477:497	the alginate hydrogel (Alg)	477:503	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	7	5	theme	composite	1100:1108	arg1	scaffold					1110:1117	the composite scaffold	1096:1117	the composite scaffold	1096:1117	RESULTS Results showed that the composite scaffold possessed superior mechanical properties and hierarchical porous structure in comparison to pure Alg.
27646631	7	6	contain	possessed	1119:1127	arg2	structure					1184:1192	hierarchical porous structure	1164:1192	hierarchical porous structure	1164:1192	RESULTS Results showed that the composite scaffold possessed superior mechanical properties and hierarchical porous structure in comparison to pure Alg.
27646631	7	6	contain	possessed	1119:1127	arg1	scaffold					1110:1117	the composite scaffold	1096:1117	the composite scaffold	1096:1117	RESULTS Results showed that the composite scaffold possessed superior mechanical properties and hierarchical porous structure in comparison to pure Alg.
27646631	7	6	contain	possessed	1119:1127	arg2	properties					1149:1158	superior mechanical properties	1129:1158	superior mechanical properties	1129:1158	RESULTS Results showed that the composite scaffold possessed superior mechanical properties and hierarchical porous structure in comparison to pure Alg.
27646631	2	7	theme	poly	358:361	arg1	scaffold					386:393	a three-dimensional poly (L-lactic acid) (PLLA) scaffold	338:393	a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores	338:449	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	9	8	theme	composite	1422:1430	arg1	scaffold					1432:1439	the composite scaffold	1418:1439	the composite scaffold	1418:1439	The histological analyses suggested that the composite scaffold substantially promotes its integration in the host tissue accompanied with a low inflammatory reaction and new tissue formation.
27646631	4	9	theme	compression	750:760	arg1	testing					762:768	compression testing	750:768	compression testing	750:768	The mechanical strength of the composite scaffold was characterized by compression testing.
27646631	7	10	theme	pure	1211:1214	arg1	Alg					1216:1218	pure Alg	1211:1218	pure Alg	1211:1218	RESULTS Results showed that the composite scaffold possessed superior mechanical properties and hierarchical porous structure in comparison to pure Alg.
27646631	1	11	theme	drug	276:279	arg1	delivery					281:288	drug delivery	276:288	drug delivery	276:288	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	4	12	theme	scaffold	720:727	arg1	strength					694:701	The mechanical strength	679:701	The mechanical strength of the composite scaffold	679:727	The mechanical strength of the composite scaffold was characterized by compression testing.
27646631	6	13	theme	composite	943:951	arg1	scaffold					953:960	such a composite scaffold	936:960	such a composite scaffold	936:960	The repair efficiency of such a composite scaffold was further investigated in dog spinal defects by histological evaluation after implantation for 4 weeks.
27646631	10	14	theme	scaffold	1628:1635	arg1	preparation					1637:1647	scaffold preparation	1628:1647	scaffold preparation	1628:1647	CONCLUSIONS The method thus provides a useful pathway for scaffold preparation that can simultaneously achieve suitable mechanical properties and good biocompatibility.
27646631	1	15	theme	PURPOSE	123:129	arg1	scaffolds					146:154	PURPOSE Polymer porous scaffolds	123:154	PURPOSE Polymer porous scaffolds	123:154	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	7	16	theme	RESULTS	1068:1074	arg1	Results					1076:1082	RESULTS Results	1068:1082	RESULTS Results	1068:1082	RESULTS Results showed that the composite scaffold possessed superior mechanical properties and hierarchical porous structure in comparison to pure Alg.
27646631	6	17	theme	histological	1012:1023	arg1	evaluation					1025:1034	histological evaluation	1012:1034	histological evaluation after implantation for 4 weeks	1012:1065	The repair efficiency of such a composite scaffold was further investigated in dog spinal defects by histological evaluation after implantation for 4 weeks.
27646631	5	18	theme	MTT	890:892	arg1	assay					904:908	MTT viability assay	890:908	MTT viability assay	890:908	The chondrocyte behavior in the scaffold was determined by inverted microscopy, scanning electron microscopy (SEM) and MTT viability assay.
27646631	5	19	theme	inverted	830:837	arg1	microscopy					839:848	inverted microscopy	830:848	inverted microscopy	830:848	The chondrocyte behavior in the scaffold was determined by inverted microscopy, scanning electron microscopy (SEM) and MTT viability assay.
27646631	10	20	dep	CONCLUSIONS	1570:1580	arg1	provides					1598:1605	provides	1598:1605	provides a useful pathway for scaffold preparation that can simultaneously achieve suitable mechanical properties and good biocompatibility	1598:1736	CONCLUSIONS The method thus provides a useful pathway for scaffold preparation that can simultaneously achieve suitable mechanical properties and good biocompatibility.
27646631	3	21	theme	shape	672:676	arg1	support					650:656	a support	648:656	a support of predefined shape	648:676	The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape.
27646631	8	22	attach	presented	1258:1266	arg1	scaffold					1313:1320	the composite scaffold	1299:1320	the composite scaffold in line with higher adherence and proliferation ratio	1299:1374	Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio.
27646631	8	22	attach	presented	1258:1266	arg2	cells					1252:1256	the cells	1248:1256	the cells	1248:1256	Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio.
27646631	6	23	theme	dog	990:992	arg1	defects					1001:1007	dog spinal defects	990:1007	dog spinal defects	990:1007	The repair efficiency of such a composite scaffold was further investigated in dog spinal defects by histological evaluation after implantation for 4 weeks.
27646631	8	24	with	line	1325:1328	arg1	ratio					1370:1374	higher adherence and proliferation ratio	1335:1374	higher adherence and proliferation ratio	1335:1374	Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio.
27646631	9	25	theme	histological	1381:1392	arg1	analyses					1394:1401	The histological analyses	1377:1401	The histological analyses	1377:1401	The histological analyses suggested that the composite scaffold substantially promotes its integration in the host tissue accompanied with a low inflammatory reaction and new tissue formation.
27646631	5	26	theme	chondrocyte	775:785	arg1	behavior					787:794	The chondrocyte behavior	771:794	The chondrocyte behavior in the scaffold	771:810	The chondrocyte behavior in the scaffold was determined by inverted microscopy, scanning electron microscopy (SEM) and MTT viability assay.
27646631	0	27	theme	improved	70:77	arg1	properties					90:99	improved mechanical properties	70:99	improved mechanical properties	70:99	Poly (L-lactic acid) porous scaffold-supported alginate hydrogel with improved mechanical properties and biocompatibility.
27646631	2	28	with	scaffold	386:393	arg1	pores					445:449	interconnected and homogeneously distributed pores	400:449	interconnected and homogeneously distributed pores	400:449	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	10	29	theme	suitable	1681:1688	arg1	properties					1701:1710	suitable mechanical properties	1681:1710	suitable mechanical properties	1681:1710	CONCLUSIONS The method thus provides a useful pathway for scaffold preparation that can simultaneously achieve suitable mechanical properties and good biocompatibility.
27646631	0	30	theme	Poly	0:3	arg1	porous					21:26	Poly (L-lactic acid) porous	0:26	Poly (L-lactic acid) porous	0:26	Poly (L-lactic acid) porous scaffold-supported alginate hydrogel with improved mechanical properties and biocompatibility.
27646631	8	31	from	scaffold	1313:1320	arg1	line					1325:1328	line	1325:1328	line with higher adherence and proliferation ratio	1325:1374	Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio.
27646631	8	32	theme	proliferation	1356:1368	arg1	ratio					1370:1374	higher adherence and proliferation ratio	1335:1374	higher adherence and proliferation ratio	1335:1374	Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio.
27646631	5	33	theme	scanning	851:858	arg1	SEM					881:883	SEM	881:883	SEM	881:883	The chondrocyte behavior in the scaffold was determined by inverted microscopy, scanning electron microscopy (SEM) and MTT viability assay.
27646631	5	33	theme	scanning	851:858	arg1	microscopy					869:878	scanning electron microscopy	851:878	scanning electron microscopy (SEM)	851:884	The chondrocyte behavior in the scaffold was determined by inverted microscopy, scanning electron microscopy (SEM) and MTT viability assay.
27646631	2	34	theme	distributed	433:443	arg1	pores					445:449	interconnected and homogeneously distributed pores	400:449	interconnected and homogeneously distributed pores	400:449	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	3	35	theme	porous	536:541	arg1	scaffold					543:550	the porous scaffold	532:550	the porous scaffold	532:550	The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape.
27646631	3	35	theme	porous	536:541	arg1	analogue					571:578	an analogue	568:578	an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape	568:676	The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape.
27646631	9	36	theme	host	1487:1490	arg1	tissue					1492:1497	the host tissue	1483:1497	the host tissue accompanied with a low inflammatory reaction and new tissue formation	1483:1567	The histological analyses suggested that the composite scaffold substantially promotes its integration in the host tissue accompanied with a low inflammatory reaction and new tissue formation.
27646631	0	37	theme	L-lactic	6:13	arg1	Poly					0:3	Poly	0:3	Poly (L-lactic acid) porous	0:26	Poly (L-lactic acid) porous scaffold-supported alginate hydrogel with improved mechanical properties and biocompatibility.
27646631	0	37	theme	L-lactic	6:13	arg1	acid					15:18	L-lactic acid	6:18	L-lactic acid	6:18	Poly (L-lactic acid) porous scaffold-supported alginate hydrogel with improved mechanical properties and biocompatibility.
27646631	1	38	theme	tissue	295:300	arg1	engineering					302:312	tissue engineering	295:312	tissue engineering	295:312	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	9	39	theme	low	1518:1520	arg1	reaction					1535:1542	a low inflammatory reaction	1516:1542	a low inflammatory reaction	1516:1542	The histological analyses suggested that the composite scaffold substantially promotes its integration in the host tissue accompanied with a low inflammatory reaction and new tissue formation.
27646631	9	40	theme	tissue	1552:1557	arg1	formation					1559:1567	new tissue formation	1548:1567	new tissue formation	1548:1567	The histological analyses suggested that the composite scaffold substantially promotes its integration in the host tissue accompanied with a low inflammatory reaction and new tissue formation.
27646631	2	41	theme	interconnected	400:413	arg1	pores					445:449	interconnected and homogeneously distributed pores	400:449	interconnected and homogeneously distributed pores	400:449	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	7	42	theme	superior	1129:1136	arg1	properties					1149:1158	superior mechanical properties	1129:1158	superior mechanical properties	1129:1158	RESULTS Results showed that the composite scaffold possessed superior mechanical properties and hierarchical porous structure in comparison to pure Alg.
27646631	8	43	theme	cartilage	1279:1287	arg1	status					1289:1294	a specific cartilage status	1268:1294	a specific cartilage status	1268:1294	Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio.
27646631	7	44	theme	porous	1177:1182	arg1	structure					1184:1192	hierarchical porous structure	1164:1192	hierarchical porous structure	1164:1192	RESULTS Results showed that the composite scaffold possessed superior mechanical properties and hierarchical porous structure in comparison to pure Alg.
27646631	1	45	theme	wide	219:222	arg1	engineering					302:312	tissue engineering	295:312	tissue engineering	295:312	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	1	45	theme	wide	219:222	arg1	delivery					281:288	drug delivery	276:288	drug delivery	276:288	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	1	45	theme	wide	219:222	arg1	encapsulation					261:273	cell encapsulation	256:273	cell encapsulation	256:273	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	1	45	theme	wide	219:222	arg1	range					224:228	a wide range	217:228	a wide range of applications including cell encapsulation, drug delivery, and tissue engineering	217:312	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	7	46	theme	mechanical	1138:1147	arg1	properties					1149:1158	superior mechanical properties	1129:1158	superior mechanical properties	1129:1158	RESULTS Results showed that the composite scaffold possessed superior mechanical properties and hierarchical porous structure in comparison to pure Alg.
27646631	6	47	theme	repair	915:920	arg1	efficiency					922:931	The repair efficiency	911:931	The repair efficiency of such a composite scaffold	911:960	The repair efficiency of such a composite scaffold was further investigated in dog spinal defects by histological evaluation after implantation for 4 weeks.
27646631	8	48	theme	composite	1303:1311	arg1	scaffold					1313:1320	the composite scaffold	1299:1320	the composite scaffold in line with higher adherence and proliferation ratio	1299:1374	Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio.
27646631	0	49	theme	alginate	47:54	arg1	hydrogel					56:63	alginate hydrogel	47:63	alginate hydrogel	47:63	Poly (L-lactic acid) porous scaffold-supported alginate hydrogel with improved mechanical properties and biocompatibility.
27646631	8	50	theme	higher	1335:1340	arg1	ratio					1370:1374	higher adherence and proliferation ratio	1335:1374	higher adherence and proliferation ratio	1335:1374	Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio.
27646631	2	51	theme	three-dimensional	340:356	arg1	PLLA					380:383	PLLA	380:383	PLLA	380:383	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	2	51	theme	three-dimensional	340:356	arg1	poly					358:361	three-dimensional poly	340:361	a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores	338:449	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	2	51	theme	three-dimensional	340:356	arg1	acid					373:376	L-lactic acid	364:376	L-lactic acid	364:376	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	2	52	dep	METHODS	315:321	arg1	study					331:335	this study	326:335	this study	326:335	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	9	53	theme	inflammatory	1522:1533	arg1	reaction					1535:1542	a low inflammatory reaction	1516:1542	a low inflammatory reaction	1516:1542	The histological analyses suggested that the composite scaffold substantially promotes its integration in the host tissue accompanied with a low inflammatory reaction and new tissue formation.
27646631	3	54	theme	cells	635:639	arg1	entrapment					621:630	entrapment	621:630	entrapment of cells within a support of predefined shape	621:676	The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape.
27646631	1	55	theme	Polymer	131:137	arg1	scaffolds					146:154	PURPOSE Polymer porous scaffolds	123:154	PURPOSE Polymer porous scaffolds	123:154	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	4	56	theme	mechanical	683:692	arg1	strength					694:701	The mechanical strength	679:701	The mechanical strength of the composite scaffold	679:727	The mechanical strength of the composite scaffold was characterized by compression testing.
27646631	8	57	theme	specific	1270:1277	arg1	status					1289:1294	a specific cartilage status	1268:1294	a specific cartilage status	1268:1294	Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio.
27646631	7	58	theme	hierarchical	1164:1175	arg1	structure					1184:1192	hierarchical porous structure	1164:1192	hierarchical porous structure	1164:1192	RESULTS Results showed that the composite scaffold possessed superior mechanical properties and hierarchical porous structure in comparison to pure Alg.
27646631	3	59	theme	extracellular	590:602	arg1	ECM					612:614	ECM	612:614	ECM	612:614	The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape.
27646631	3	59	theme	extracellular	590:602	arg1	matrix					604:609	native extracellular matrix	583:609	native extracellular matrix (ECM)	583:615	The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape.
27646631	10	60	theme	good	1716:1719	arg1	biocompatibility					1721:1736	good biocompatibility	1716:1736	good biocompatibility	1716:1736	CONCLUSIONS The method thus provides a useful pathway for scaffold preparation that can simultaneously achieve suitable mechanical properties and good biocompatibility.
27646631	1	61	theme	porous	139:144	arg1	scaffolds					146:154	PURPOSE Polymer porous scaffolds	123:154	PURPOSE Polymer porous scaffolds	123:154	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	4	62	theme	composite	710:718	arg1	scaffold					720:727	the composite scaffold	706:727	the composite scaffold	706:727	The mechanical strength of the composite scaffold was characterized by compression testing.
27646631	6	63	theme	spinal	994:999	arg1	defects					1001:1007	dog spinal defects	990:1007	dog spinal defects	990:1007	The repair efficiency of such a composite scaffold was further investigated in dog spinal defects by histological evaluation after implantation for 4 weeks.
27646631	8	64	theme	adherence	1342:1350	arg1	ratio					1370:1374	higher adherence and proliferation ratio	1335:1374	higher adherence and proliferation ratio	1335:1374	Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio.
27646631	3	65	theme	matrix	604:609	arg1	scaffold					543:550	the porous scaffold	532:550	the porous scaffold	532:550	The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape.
27646631	3	65	theme	matrix	604:609	arg1	analogue					571:578	an analogue	568:578	an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape	568:676	The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape.
27646631	9	66	from	integration	1468:1478	arg1	tissue					1492:1497	the host tissue	1483:1497	the host tissue accompanied with a low inflammatory reaction and new tissue formation	1483:1567	The histological analyses suggested that the composite scaffold substantially promotes its integration in the host tissue accompanied with a low inflammatory reaction and new tissue formation.
27646631	5	67	theme	viability	894:902	arg1	assay					904:908	MTT viability assay	890:908	MTT viability assay	890:908	The chondrocyte behavior in the scaffold was determined by inverted microscopy, scanning electron microscopy (SEM) and MTT viability assay.
27646631	8	68	theme	Cell	1221:1224	arg1	culture					1226:1232	Cell culture	1221:1232	Cell culture	1221:1232	Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio.
27646631	1	69	theme	applications	233:244	arg1	engineering					302:312	tissue engineering	295:312	tissue engineering	295:312	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	1	69	theme	applications	233:244	arg1	delivery					281:288	drug delivery	276:288	drug delivery	276:288	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	1	69	theme	applications	233:244	arg1	encapsulation					261:273	cell encapsulation	256:273	cell encapsulation	256:273	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	1	69	theme	applications	233:244	arg1	range					224:228	a wide range	217:228	a wide range of applications including cell encapsulation, drug delivery, and tissue engineering	217:312	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27646631	5	70	theme	electron	860:867	arg1	SEM					881:883	SEM	881:883	SEM	881:883	The chondrocyte behavior in the scaffold was determined by inverted microscopy, scanning electron microscopy (SEM) and MTT viability assay.
27646631	5	70	theme	electron	860:867	arg1	microscopy					869:878	scanning electron microscopy	851:878	scanning electron microscopy (SEM)	851:884	The chondrocyte behavior in the scaffold was determined by inverted microscopy, scanning electron microscopy (SEM) and MTT viability assay.
27646631	10	71	theme	mechanical	1690:1699	arg1	properties					1701:1710	suitable mechanical properties	1681:1710	suitable mechanical properties	1681:1710	CONCLUSIONS The method thus provides a useful pathway for scaffold preparation that can simultaneously achieve suitable mechanical properties and good biocompatibility.
27646631	6	72	theme	scaffold	953:960	arg1	efficiency					922:931	The repair efficiency	911:931	The repair efficiency of such a composite scaffold	911:960	The repair efficiency of such a composite scaffold was further investigated in dog spinal defects by histological evaluation after implantation for 4 weeks.
27646631	3	73	theme	native	583:588	arg1	ECM					612:614	ECM	612:614	ECM	612:614	The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape.
27646631	3	73	theme	native	583:588	arg1	matrix					604:609	native extracellular matrix	583:609	native extracellular matrix (ECM)	583:615	The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape.
27646631	10	74	theme	useful	1609:1614	arg1	pathway					1616:1622	a useful pathway	1607:1622	a useful pathway for scaffold preparation that can simultaneously achieve suitable mechanical properties and good biocompatibility	1607:1736	CONCLUSIONS The method thus provides a useful pathway for scaffold preparation that can simultaneously achieve suitable mechanical properties and good biocompatibility.
27646631	2	75	theme	L-lactic	364:371	arg1	poly					358:361	three-dimensional poly	340:361	a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores	338:449	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	2	75	theme	L-lactic	364:371	arg1	acid					373:376	L-lactic acid	364:376	L-lactic acid	364:376	METHODS In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg).
27646631	1	76	theme	cell	256:259	arg1	encapsulation					261:273	cell encapsulation	256:273	cell encapsulation	256:273	PURPOSE Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.
27234816	4	0	theme	biomedical	910:919	arg1	applications					921:932	biomedical applications	910:932	biomedical applications	910:932	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	1	1	theme	drug	163:166	arg1	delivery					168:175	sequential and/or sustained drug delivery	135:175	sequential and/or sustained drug delivery	135:175	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	3	2	dep	line	725:728	arg1	cells					758:762	stromal stem cells	745:762	stromal stem cells (BMSC)	745:769	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	3	2	dep	line	725:728	arg1	BMSC					765:768	BMSC	765:768	BMSC	765:768	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	1	3	theme	drugs	248:252	arg1	effects					224:230	the therapeutic effects	208:230	the therapeutic effects of many current drugs	208:252	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	2	4	theme	delivery	462:469	arg1	functionality					471:483	sequential delivery functionality	451:483	sequential delivery functionality	451:483	In this study, with the aid of 3D printing technology, a novel drug delivery device was fabricated and tested to evaluate sequential delivery functionality.
27234816	4	5	theme	drugs	811:815	arg1	release					800:806	The controlled differential release	772:806	The controlled differential release of drugs or proteins through such a delivery system	772:858	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	3	6	theme	alginate	494:501	arg1	shell					503:507	an alginate shell	491:507	an alginate shell	491:507	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	2	7	dep	the	349:351	arg1	aid					353:355	aid	353:355	aid	353:355	In this study, with the aid of 3D printing technology, a novel drug delivery device was fabricated and tested to evaluate sequential delivery functionality.
27234816	3	8	theme	embryonic	694:702	arg1	HEK293					712:717	HEK293	712:717	HEK293	712:717	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	3	8	theme	embryonic	694:702	arg1	kidney					704:709	the human embryonic kidney	684:709	the human embryonic kidney (HEK293) cell line	684:728	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	4	9	theme	proteins	820:827	arg1	release					800:806	The controlled differential release	772:806	The controlled differential release of drugs or proteins through such a delivery system	772:858	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	3	10	theme	lactic-co-glycolic	520:537	arg1	poly					515:518	a poly	513:518	a poly(lactic-co-glycolic acid) (PLGA) core	513:555	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	3	10	theme	lactic-co-glycolic	520:537	arg1	acid					539:542	lactic-co-glycolic acid	520:542	lactic-co-glycolic acid	520:542	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	3	11	theme	kidney	704:709	arg1	line					725:728	the human embryonic kidney (HEK293) cell line	684:728	the human embryonic kidney (HEK293) cell line	684:728	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	4	12	theme	differential	787:798	arg1	release					800:806	The controlled differential release	772:806	The controlled differential release of drugs or proteins through such a delivery system	772:858	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	2	13	theme	sequential	451:460	arg1	functionality					471:483	sequential delivery functionality	451:483	sequential delivery functionality	451:483	In this study, with the aid of 3D printing technology, a novel drug delivery device was fabricated and tested to evaluate sequential delivery functionality.
27234816	3	14	theme	poly	515:518	arg1	core					552:555	a poly(lactic-co-glycolic acid) (PLGA) core	513:555	a poly(lactic-co-glycolic acid) (PLGA) core	513:555	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	3	15	theme	fluorescent	620:630	arg1	dyes					632:635	distinct fluorescent dyes	611:635	distinct fluorescent dyes	611:635	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	3	16	theme	human	688:692	arg1	HEK293					712:717	HEK293	712:717	HEK293	712:717	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	3	16	theme	human	688:692	arg1	kidney					704:709	the human embryonic kidney	684:709	the human embryonic kidney (HEK293) cell line	684:728	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	1	17	theme	Controlled	88:97	arg1	systems					113:119	Controlled drug delivery systems	88:119	Controlled drug delivery systems	88:119	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	2	18	theme	printing	363:370	arg1	technology					372:381	3D printing technology	360:381	3D printing technology	360:381	In this study, with the aid of 3D printing technology, a novel drug delivery device was fabricated and tested to evaluate sequential delivery functionality.
27234816	0	19	theme	Controlled	0:9	arg1	Delivery					26:33	Controlled and Sequential Delivery	0:33	Controlled and Sequential Delivery of Fluorophores from 3D Printed Alginate-PLGA Tubes.	0:86	Controlled and Sequential Delivery of Fluorophores from 3D Printed Alginate-PLGA Tubes.
27234816	1	20	theme	drug	99:102	arg1	systems					113:119	Controlled drug delivery systems	88:119	Controlled drug delivery systems	88:119	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	2	21	theme	3D	360:361	arg1	technology					372:381	3D printing technology	360:381	3D printing technology	360:381	In this study, with the aid of 3D printing technology, a novel drug delivery device was fabricated and tested to evaluate sequential delivery functionality.
27234816	3	22	theme	cell	720:723	arg1	line					725:728	the human embryonic kidney (HEK293) cell line	684:728	the human embryonic kidney (HEK293) cell line	684:728	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	1	23	theme	delivery	104:111	arg1	systems					113:119	Controlled drug delivery systems	88:119	Controlled drug delivery systems	88:119	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	2	24	theme	delivery	397:404	arg1	device					406:411	a novel drug delivery device	384:411	a novel drug delivery device	384:411	In this study, with the aid of 3D printing technology, a novel drug delivery device was fabricated and tested to evaluate sequential delivery functionality.
27234816	0	25	theme	Sequential	15:24	arg1	Delivery					26:33	Controlled and Sequential Delivery	0:33	Controlled and Sequential Delivery of Fluorophores from 3D Printed Alginate-PLGA Tubes.	0:86	Controlled and Sequential Delivery of Fluorophores from 3D Printed Alginate-PLGA Tubes.
27234816	4	26	theme	regenerative	958:969	arg1	medicine					971:978	regenerative medicine	958:978	regenerative medicine	958:978	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	3	27	theme	distinct	611:618	arg1	dyes					632:635	distinct fluorescent dyes	611:635	distinct fluorescent dyes	611:635	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	4	28	contain	has	860:862	arg1	release					800:806	The controlled differential release	772:806	The controlled differential release of drugs or proteins through such a delivery system	772:858	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	4	28	contain	has	860:862	arg2	potential					868:876	the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine	864:978	the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine	864:978	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	2	29	theme	drug	392:395	arg1	device					406:411	a novel drug delivery device	384:411	a novel drug delivery device	384:411	In this study, with the aid of 3D printing technology, a novel drug delivery device was fabricated and tested to evaluate sequential delivery functionality.
27234816	0	30	theme	Fluorophores	38:49	arg1	Delivery					26:33	Controlled and Sequential Delivery	0:33	Controlled and Sequential Delivery of Fluorophores from 3D Printed Alginate-PLGA Tubes.	0:86	Controlled and Sequential Delivery of Fluorophores from 3D Printed Alginate-PLGA Tubes.
27234816	1	31	theme	time-dependent	291:304	arg1	manner					321:326	a time-dependent and repeatable manner	289:326	a time-dependent and repeatable manner	289:326	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	0	32	from	Tubes	81:85	arg1	Delivery					26:33	Controlled and Sequential Delivery	0:33	Controlled and Sequential Delivery of Fluorophores from 3D Printed Alginate-PLGA Tubes.	0:86	Controlled and Sequential Delivery of Fluorophores from 3D Printed Alginate-PLGA Tubes.
27234816	2	33	theme	novel	386:390	arg1	device					406:411	a novel drug delivery device	384:411	a novel drug delivery device	384:411	In this study, with the aid of 3D printing technology, a novel drug delivery device was fabricated and tested to evaluate sequential delivery functionality.
27234816	4	34	theme	applications	921:932	arg1	variety					899:905	a wide variety	892:905	a wide variety of biomedical applications	892:932	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	4	34	theme	applications	921:932	arg1	applications					921:932	biomedical applications	910:932	biomedical applications	910:932	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	3	35	theme	bone	733:736	arg1	marrow					738:743	bone marrow	733:743	bone marrow	733:743	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	3	36	theme	stromal	745:751	arg1	cells					758:762	stromal stem cells	745:762	stromal stem cells (BMSC)	745:769	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	3	36	theme	stromal	745:751	arg1	BMSC					765:768	BMSC	765:768	BMSC	765:768	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	1	37	theme	therapeutic	212:222	arg1	effects					224:230	the therapeutic effects	208:230	the therapeutic effects of many current drugs	208:252	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	0	38	theme	3D	56:57	arg1	Tubes					81:85	3D Printed Alginate-PLGA Tubes	56:85	3D Printed Alginate-PLGA Tubes	56:85	Controlled and Sequential Delivery of Fluorophores from 3D Printed Alginate-PLGA Tubes.
27234816	1	39	theme	repeatable	310:319	arg1	manner					321:326	a time-dependent and repeatable manner	289:326	a time-dependent and repeatable manner	289:326	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	4	40	theme	wide	894:897	arg1	variety					899:905	a wide variety	892:905	a wide variety of biomedical applications	892:932	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	4	40	theme	wide	894:897	arg1	applications					921:932	biomedical applications	910:932	biomedical applications	910:932	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	4	41	theme	controlled	776:785	arg1	release					800:806	The controlled differential release	772:806	The controlled differential release of drugs or proteins through such a delivery system	772:858	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	1	42	used	utilized	188:195	arg2	systems					113:119	Controlled drug delivery systems	88:119	Controlled drug delivery systems	88:119	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	4	43	theme	delivery	844:851	arg1	system					853:858	such a delivery system	837:858	such a delivery system	837:858	The controlled differential release of drugs or proteins through such a delivery system has the potential to be used in a wide variety of biomedical applications from treating cancer to regenerative medicine.
27234816	3	44	theme	dyes	632:635	arg1	release					600:606	sequential release	589:606	sequential release of distinct fluorescent dyes	589:635	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	3	45	theme	sequential	589:598	arg1	release					600:606	sequential release	589:606	sequential release of distinct fluorescent dyes	589:635	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	1	46	theme	sequential	135:144	arg1	delivery					168:175	sequential and/or sustained drug delivery	135:175	sequential and/or sustained drug delivery	135:175	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	0	47	theme	Alginate-PLGA	67:79	arg1	Tubes					81:85	3D Printed Alginate-PLGA Tubes	56:85	3D Printed Alginate-PLGA Tubes	56:85	Controlled and Sequential Delivery of Fluorophores from 3D Printed Alginate-PLGA Tubes.
27234816	3	48	theme	fabricated	562:571	arg1	tubes					573:577	the fabricated tubes	558:577	the fabricated tubes	558:577	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	1	49	theme	many	235:238	arg1	drugs					248:252	many current drugs	235:252	many current drugs	235:252	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	0	50	theme	Printed	59:65	arg1	Tubes					81:85	3D Printed Alginate-PLGA Tubes	56:85	3D Printed Alginate-PLGA Tubes	56:85	Controlled and Sequential Delivery of Fluorophores from 3D Printed Alginate-PLGA Tubes.
27234816	3	51	theme	stem	753:756	arg1	cells					758:762	stromal stem cells	745:762	stromal stem cells (BMSC)	745:769	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	3	51	theme	stem	753:756	arg1	BMSC					765:768	BMSC	765:768	BMSC	765:768	With an alginate shell and a poly(lactic-co-glycolic acid) (PLGA) core, the fabricated tubes displayed sequential release of distinct fluorescent dyes and showed no cytotoxicity when incubated with the human embryonic kidney (HEK293) cell line or bone marrow stromal stem cells (BMSC).
27234816	1	52	theme	sustained	153:161	arg1	delivery					168:175	sequential and/or sustained drug delivery	135:175	sequential and/or sustained drug delivery	135:175	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
27234816	1	53	theme	current	240:246	arg1	drugs					248:252	many current drugs	235:252	many current drugs	235:252	Controlled drug delivery systems, that include sequential and/or sustained drug delivery, have been utilized to enhance the therapeutic effects of many current drugs by effectively delivering drugs in a time-dependent and repeatable manner.
24433914	4	0	theme	obtained	816:823	arg1	hydrogel					835:842	the as obtained composite hydrogel	809:842	the as obtained composite hydrogel	809:842	In addition, the as obtained composite hydrogel was also evaluated for its in vitro cytotoxicity on L929 mouse fibroblast cells and was confirmed to have a good biocompatibility.
24433914	1	1	from	protein	417:423	arg1	system					436:441	aqueous system	428:441	aqueous system	428:441	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	1	2	theme	in-situ	166:172	arg1	encapsulation					174:186	in-situ encapsulation	166:186	in-situ encapsulation	166:186	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	1	3	theme	aldehyde	273:280	arg1	groups					282:287	aldehyde groups	273:287	aldehyde groups	273:287	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	0	4	theme	drug	99:102	arg1	release					104:110	controlled drug release	88:110	controlled drug release	88:110	Using casein and oxidized hyaluronic acid to form biocompatible composite hydrogels for controlled drug release.
24433914	3	5	theme	aldehyde	634:641	arg1	content					649:655	its aldehyde group content	630:655	its aldehyde group content	630:655	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
24433914	3	6	theme	prolonged	772:780	arg1	release					787:793	a prolonged drug release	770:793	a prolonged drug release	770:793	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
24433914	0	7	theme	controlled	88:97	arg1	release					104:110	controlled drug release	88:110	controlled drug release	88:110	Using casein and oxidized hyaluronic acid to form biocompatible composite hydrogels for controlled drug release.
24433914	3	8	theme	gelation	700:707	arg1	time					709:712	a shorten gelation time	690:712	a shorten gelation time	690:712	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
24433914	4	9	theme	composite	825:833	arg1	hydrogel					835:842	the as obtained composite hydrogel	809:842	the as obtained composite hydrogel	809:842	In addition, the as obtained composite hydrogel was also evaluated for its in vitro cytotoxicity on L929 mouse fibroblast cells and was confirmed to have a good biocompatibility.
24433914	3	10	theme	drug	782:785	arg1	release					787:793	a prolonged drug release	770:793	a prolonged drug release	770:793	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
24433914	4	11	theme	fibroblast	907:916	arg1	cells					918:922	L929 mouse fibroblast cells	896:922	L929 mouse fibroblast cells	896:922	In addition, the as obtained composite hydrogel was also evaluated for its in vitro cytotoxicity on L929 mouse fibroblast cells and was confirmed to have a good biocompatibility.
24433914	4	12	contain	have	945:948	arg2	biocompatibility					957:972	a good biocompatibility	950:972	a good biocompatibility	950:972	In addition, the as obtained composite hydrogel was also evaluated for its in vitro cytotoxicity on L929 mouse fibroblast cells and was confirmed to have a good biocompatibility.
24433914	4	12	contain	have	945:948	arg1	hydrogel					835:842	the as obtained composite hydrogel	809:842	the as obtained composite hydrogel	809:842	In addition, the as obtained composite hydrogel was also evaluated for its in vitro cytotoxicity on L929 mouse fibroblast cells and was confirmed to have a good biocompatibility.
24433914	4	13	theme	mouse	901:905	arg1	cells					918:922	L929 mouse fibroblast cells	896:922	L929 mouse fibroblast cells	896:922	In addition, the as obtained composite hydrogel was also evaluated for its in vitro cytotoxicity on L929 mouse fibroblast cells and was confirmed to have a good biocompatibility.
24433914	4	14	dep	in	871:872	arg1	vitro					874:878	vitro	874:878	vitro	874:878	In addition, the as obtained composite hydrogel was also evaluated for its in vitro cytotoxicity on L929 mouse fibroblast cells and was confirmed to have a good biocompatibility.
24433914	3	15	theme	shorten	692:698	arg1	time					709:712	a shorten gelation time	690:712	a shorten gelation time	690:712	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
24433914	4	16	theme	L929	896:899	arg1	cells					918:922	L929 mouse fibroblast cells	896:922	L929 mouse fibroblast cells	896:922	In addition, the as obtained composite hydrogel was also evaluated for its in vitro cytotoxicity on L929 mouse fibroblast cells and was confirmed to have a good biocompatibility.
24433914	1	17	theme	controlled	192:201	arg1	release					203:209	controlled release	192:209	controlled release	192:209	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	3	18	theme	enhanced	718:725	arg1	strength					731:738	an enhanced gel strength	715:738	an enhanced gel strength	715:738	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
24433914	4	19	theme	good	952:955	arg1	biocompatibility					957:972	a good biocompatibility	950:972	a good biocompatibility	950:972	In addition, the as obtained composite hydrogel was also evaluated for its in vitro cytotoxicity on L929 mouse fibroblast cells and was confirmed to have a good biocompatibility.
24433914	3	20	theme	amount	660:665	arg1	increase					618:625	an increase	615:625	an increase of its aldehyde group content or amount	615:665	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
24433914	0	21	theme	oxidized	17:24	arg1	acid					37:40	oxidized hyaluronic acid	17:40	oxidized hyaluronic acid	17:40	Using casein and oxidized hyaluronic acid to form biocompatible composite hydrogels for controlled drug release.
24433914	3	22	theme	gel	727:729	arg1	strength					731:738	an enhanced gel strength	715:738	an enhanced gel strength	715:738	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
24433914	4	23	theme	in	871:872	arg1	cytotoxicity					880:891	its in vitro cytotoxicity	867:891	its in vitro cytotoxicity	867:891	In addition, the as obtained composite hydrogel was also evaluated for its in vitro cytotoxicity on L929 mouse fibroblast cells and was confirmed to have a good biocompatibility.
24433914	2	24	theme	gelation	512:519	arg1	kinetics					521:528	the gelation kinetics	508:528	the gelation kinetics	508:528	By changing its aldehyde group content or amount, we found that the gelation kinetics and the properties of resultant composite hydrogel could be modulated.
24433914	1	25	used	used	373:376	arg2	acid					257:260	the oxidized hyaluronic acid	233:260	the oxidized hyaluronic acid containing aldehyde groups	233:287	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	1	26	theme	first	386:390	arg1	time					392:395	the first time	382:395	the first time	382:395	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	1	27	theme	hydrophilic	214:224	arg1	drugs					226:230	hydrophilic drugs	214:230	hydrophilic drugs	214:230	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	3	28	theme	group	643:647	arg1	content					649:655	its aldehyde group content	630:655	its aldehyde group content	630:655	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
24433914	1	29	theme	drugs	226:230	arg1	encapsulation					174:186	in-situ encapsulation	166:186	in-situ encapsulation	166:186	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	1	29	theme	drugs	226:230	arg1	release					203:209	controlled release	192:209	controlled release	192:209	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	0	30	theme	hyaluronic	26:35	arg1	acid					37:40	oxidized hyaluronic acid	17:40	oxidized hyaluronic acid	17:40	Using casein and oxidized hyaluronic acid to form biocompatible composite hydrogels for controlled drug release.
24433914	2	31	theme	group	469:473	arg1	content					475:481	its aldehyde group content	456:481	its aldehyde group content	456:481	By changing its aldehyde group content or amount, we found that the gelation kinetics and the properties of resultant composite hydrogel could be modulated.
24433914	3	32	theme	swelling	751:758	arg1	ratio					760:764	a reduced swelling ratio	741:764	a reduced swelling ratio	741:764	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
24433914	2	33	theme	aldehyde	460:467	arg1	content					475:481	its aldehyde group content	456:481	its aldehyde group content	456:481	By changing its aldehyde group content or amount, we found that the gelation kinetics and the properties of resultant composite hydrogel could be modulated.
24433914	1	34	theme	hyaluronic	326:335	arg1	acid					337:340	hyaluronic acid	326:340	hyaluronic acid	326:340	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	2	35	theme	hydrogel	572:579	arg1	kinetics					521:528	the gelation kinetics	508:528	the gelation kinetics	508:528	By changing its aldehyde group content or amount, we found that the gelation kinetics and the properties of resultant composite hydrogel could be modulated.
24433914	2	35	theme	hydrogel	572:579	arg1	properties					538:547	the properties	534:547	the properties of resultant composite hydrogel	534:579	By changing its aldehyde group content or amount, we found that the gelation kinetics and the properties of resultant composite hydrogel could be modulated.
24433914	1	36	dep	encapsulation	174:186	arg1	the					162:164	the	162:164	the	162:164	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	2	37	theme	composite	562:570	arg1	hydrogel					572:579	resultant composite hydrogel	552:579	resultant composite hydrogel	552:579	By changing its aldehyde group content or amount, we found that the gelation kinetics and the properties of resultant composite hydrogel could be modulated.
24433914	1	38	theme	casein	410:415	arg1	protein					417:423	casein protein	410:423	casein protein in aqueous system	410:441	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	2	39	theme	resultant	552:560	arg1	hydrogel					572:579	resultant composite hydrogel	552:579	resultant composite hydrogel	552:579	By changing its aldehyde group content or amount, we found that the gelation kinetics and the properties of resultant composite hydrogel could be modulated.
24433914	1	40	theme	biocompatible	124:136	arg1	hydrogels					148:156	biocompatible polymeric hydrogels	124:156	biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs	124:230	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	3	41	theme	content	649:655	arg1	increase					618:625	an increase	615:625	an increase of its aldehyde group content or amount	615:665	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
24433914	1	42	theme	polymeric	138:146	arg1	hydrogels					148:156	biocompatible polymeric hydrogels	124:156	biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs	124:230	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	1	43	theme	oxidized	237:244	arg1	acid					257:260	the oxidized hyaluronic acid	233:260	the oxidized hyaluronic acid containing aldehyde groups	233:287	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	0	44	theme	composite	64:72	arg1	hydrogels					74:82	biocompatible composite hydrogels	50:82	biocompatible composite hydrogels for controlled drug release	50:110	Using casein and oxidized hyaluronic acid to form biocompatible composite hydrogels for controlled drug release.
24433914	1	45	theme	aqueous	428:434	arg1	system					436:441	aqueous system	428:441	aqueous system	428:441	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	1	46	theme	hyaluronic	246:255	arg1	acid					257:260	the oxidized hyaluronic acid	233:260	the oxidized hyaluronic acid containing aldehyde groups	233:287	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	0	47	theme	biocompatible	50:62	arg1	hydrogels					74:82	biocompatible composite hydrogels	50:82	biocompatible composite hydrogels for controlled drug release	50:110	Using casein and oxidized hyaluronic acid to form biocompatible composite hydrogels for controlled drug release.
24433914	1	48	theme	sodium	346:351	arg1	periodate					353:361	sodium periodate	346:361	sodium periodate	346:361	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	1	49	contain	containing	262:271	arg1	acid					257:260	the oxidized hyaluronic acid	233:260	the oxidized hyaluronic acid containing aldehyde groups	233:287	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	1	49	contain	containing	262:271	arg2	groups					282:287	aldehyde groups	273:287	aldehyde groups	273:287	To develop biocompatible polymeric hydrogels for the in-situ encapsulation and controlled release of hydrophilic drugs, the oxidized hyaluronic acid containing aldehyde groups was prepared by the reaction between hyaluronic acid and sodium periodate, and then used for the first time to crosslink casein protein in aqueous system.
24433914	3	50	theme	reduced	743:749	arg1	ratio					760:764	a reduced swelling ratio	741:764	a reduced swelling ratio	741:764	Particularly, an increase of its aldehyde group content or amount was found to result in a shorten gelation time, an enhanced gel strength, a reduced swelling ratio and a prolonged drug release.
28756077	2	0	theme	events	306:311	arg1	occurrence					277:286	the occurrence	273:286	the occurrence of cardiovascular events	273:311	The circulating level of TMAO, a metabolite of the gut microbiota, is directly correlated to the occurrence of cardiovascular events.
28756077	5	1	theme	promising	643:651	arg1	fields					662:667	promising research fields	643:667	promising research fields	643:667	Understanding the implication of the gut microbiota and developing new therapeutic strategies are promising research fields to manage metabolic and cardiovascular diseases.
28756077	2	2	theme	cardiovascular	291:304	arg1	events					306:311	cardiovascular events	291:311	cardiovascular events	291:311	The circulating level of TMAO, a metabolite of the gut microbiota, is directly correlated to the occurrence of cardiovascular events.
28756077	5	3	theme	research	653:660	arg1	fields					662:667	promising research fields	643:667	promising research fields	643:667	Understanding the implication of the gut microbiota and developing new therapeutic strategies are promising research fields to manage metabolic and cardiovascular diseases.
28756077	5	4	theme	gut	582:584	arg1	microbiota					586:595	the gut microbiota	578:595	the gut microbiota	578:595	Understanding the implication of the gut microbiota and developing new therapeutic strategies are promising research fields to manage metabolic and cardiovascular diseases.
28756077	2	5	theme	microbiota	235:244	arg1	TMAO					205:208	TMAO	205:208	TMAO	205:208	The circulating level of TMAO, a metabolite of the gut microbiota, is directly correlated to the occurrence of cardiovascular events.
28756077	2	5	theme	microbiota	235:244	arg1	metabolite					213:222	a metabolite	211:222	a metabolite of the gut microbiota	211:244	The circulating level of TMAO, a metabolite of the gut microbiota, is directly correlated to the occurrence of cardiovascular events.
28756077	3	6	theme	Bile	314:317	arg1	acids					319:323	Bile acids	314:323	Bile acids	314:323	Bile acids are protective metabolites against cardiovascular diseases through their anti-inflammatory and anti-atherogenic effects.
28756077	3	6	theme	Bile	314:317	arg1	metabolites					340:350	protective metabolites	329:350	protective metabolites against cardiovascular diseases	329:382	Bile acids are protective metabolites against cardiovascular diseases through their anti-inflammatory and anti-atherogenic effects.
28756077	5	7	theme	microbiota	586:595	arg1	implication					563:573	the implication	559:573	the implication of the gut microbiota	559:595	Understanding the implication of the gut microbiota and developing new therapeutic strategies are promising research fields to manage metabolic and cardiovascular diseases.
28756077	2	8	theme	gut	231:233	arg1	microbiota					235:244	the gut microbiota	227:244	the gut microbiota	227:244	The circulating level of TMAO, a metabolite of the gut microbiota, is directly correlated to the occurrence of cardiovascular events.
28756077	3	9	theme	cardiovascular	360:373	arg1	diseases					375:382	cardiovascular diseases	360:382	cardiovascular diseases	360:382	Bile acids are protective metabolites against cardiovascular diseases through their anti-inflammatory and anti-atherogenic effects.
28756077	5	10	theme	new	612:614	arg1	strategies					628:637	new therapeutic strategies	612:637	new therapeutic strategies	612:637	Understanding the implication of the gut microbiota and developing new therapeutic strategies are promising research fields to manage metabolic and cardiovascular diseases.
28756077	3	11	theme	anti-atherogenic	420:435	arg1	effects					437:443	their anti-inflammatory and anti-atherogenic effects	392:443	their anti-inflammatory and anti-atherogenic effects	392:443	Bile acids are protective metabolites against cardiovascular diseases through their anti-inflammatory and anti-atherogenic effects.
28756077	0	12	theme	gut	5:7	arg1	microbiota					9:18	[The gut microbiota	0:18	[The gut microbiota	0:18	[The gut microbiota, a new cardiovascular risk factor?]
28756077	0	12	theme	gut	5:7	arg1	factor					47:52	a new cardiovascular risk factor	21:52	a new cardiovascular risk factor	21:52	[The gut microbiota, a new cardiovascular risk factor?]
28756077	5	13	theme	therapeutic	616:626	arg1	strategies					628:637	new therapeutic strategies	612:637	new therapeutic strategies	612:637	Understanding the implication of the gut microbiota and developing new therapeutic strategies are promising research fields to manage metabolic and cardiovascular diseases.
28756077	1	14	theme	gut	60:62	arg1	microbiota					64:73	The gut microbiota	56:73	The gut microbiota	56:73	The gut microbiota is considered as our other "brain" and is implicated in several regulation of physiological metabolisms.
28756077	1	14	theme	gut	60:62	arg1	"					108:108	our other "brain"	92:108	our other "brain"	92:108	The gut microbiota is considered as our other "brain" and is implicated in several regulation of physiological metabolisms.
28756077	1	15	theme	several	131:137	arg1	regulation					139:148	several regulation	131:148	several regulation of physiological metabolisms	131:177	The gut microbiota is considered as our other "brain" and is implicated in several regulation of physiological metabolisms.
28756077	0	16	theme	[The	0:3	arg1	microbiota					9:18	[The gut microbiota	0:18	[The gut microbiota	0:18	[The gut microbiota, a new cardiovascular risk factor?]
28756077	0	16	theme	[The	0:3	arg1	factor					47:52	a new cardiovascular risk factor	21:52	a new cardiovascular risk factor	21:52	[The gut microbiota, a new cardiovascular risk factor?]
28756077	4	17	theme	dysbiosis	533:541	arg1	"					542:542	dysbiosis"	533:542	dysbiosis"	533:542	The disturbance in the metabolism and the composition of the gut microbiota is called "dysbiosis".
28756077	3	18	theme	anti-inflammatory	398:414	arg1	effects					437:443	their anti-inflammatory and anti-atherogenic effects	392:443	their anti-inflammatory and anti-atherogenic effects	392:443	Bile acids are protective metabolites against cardiovascular diseases through their anti-inflammatory and anti-atherogenic effects.
28756077	4	19	from	disturbance	450:460	arg1	metabolism					469:478	the metabolism	465:478	the metabolism	465:478	The disturbance in the metabolism and the composition of the gut microbiota is called "dysbiosis".
28756077	4	19	from	disturbance	450:460	arg1	composition					488:498	the composition	484:498	the composition of the gut microbiota	484:520	The disturbance in the metabolism and the composition of the gut microbiota is called "dysbiosis".
28756077	1	20	theme	physiological	153:165	arg1	metabolisms					167:177	physiological metabolisms	153:177	physiological metabolisms	153:177	The gut microbiota is considered as our other "brain" and is implicated in several regulation of physiological metabolisms.
28756077	0	21	theme	new	23:25	arg1	microbiota					9:18	[The gut microbiota	0:18	[The gut microbiota	0:18	[The gut microbiota, a new cardiovascular risk factor?]
28756077	0	21	theme	new	23:25	arg1	factor					47:52	a new cardiovascular risk factor	21:52	a new cardiovascular risk factor	21:52	[The gut microbiota, a new cardiovascular risk factor?]
28756077	1	22	theme	metabolisms	167:177	arg1	regulation					139:148	several regulation	131:148	several regulation of physiological metabolisms	131:177	The gut microbiota is considered as our other "brain" and is implicated in several regulation of physiological metabolisms.
28756077	4	23	theme	microbiota	511:520	arg1	metabolism					469:478	the metabolism	465:478	the metabolism	465:478	The disturbance in the metabolism and the composition of the gut microbiota is called "dysbiosis".
28756077	4	23	theme	microbiota	511:520	arg1	composition					488:498	the composition	484:498	the composition of the gut microbiota	484:520	The disturbance in the metabolism and the composition of the gut microbiota is called "dysbiosis".
28756077	0	24	theme	risk	42:45	arg1	microbiota					9:18	[The gut microbiota	0:18	[The gut microbiota	0:18	[The gut microbiota, a new cardiovascular risk factor?]
28756077	0	24	theme	risk	42:45	arg1	factor					47:52	a new cardiovascular risk factor	21:52	a new cardiovascular risk factor	21:52	[The gut microbiota, a new cardiovascular risk factor?]
28756077	4	25	theme	gut	507:509	arg1	microbiota					511:520	the gut microbiota	503:520	the gut microbiota	503:520	The disturbance in the metabolism and the composition of the gut microbiota is called "dysbiosis".
28756077	2	26	theme	circulating	184:194	arg1	level					196:200	The circulating level	180:200	The circulating level of TMAO, a metabolite of the gut microbiota,	180:245	The circulating level of TMAO, a metabolite of the gut microbiota, is directly correlated to the occurrence of cardiovascular events.
28756077	0	27	theme	cardiovascular	27:40	arg1	microbiota					9:18	[The gut microbiota	0:18	[The gut microbiota	0:18	[The gut microbiota, a new cardiovascular risk factor?]
28756077	0	27	theme	cardiovascular	27:40	arg1	factor					47:52	a new cardiovascular risk factor	21:52	a new cardiovascular risk factor	21:52	[The gut microbiota, a new cardiovascular risk factor?]
28756077	5	28	theme	cardiovascular	693:706	arg1	diseases					708:715	metabolic and cardiovascular diseases	679:715	metabolic and cardiovascular diseases	679:715	Understanding the implication of the gut microbiota and developing new therapeutic strategies are promising research fields to manage metabolic and cardiovascular diseases.
28756077	5	29	theme	metabolic	679:687	arg1	diseases					708:715	metabolic and cardiovascular diseases	679:715	metabolic and cardiovascular diseases	679:715	Understanding the implication of the gut microbiota and developing new therapeutic strategies are promising research fields to manage metabolic and cardiovascular diseases.
28756077	1	30	theme	other	96:100	arg1	"					108:108	our other "brain"	92:108	our other "brain"	92:108	The gut microbiota is considered as our other "brain" and is implicated in several regulation of physiological metabolisms.
28756077	1	30	theme	other	96:100	arg1	microbiota					64:73	The gut microbiota	56:73	The gut microbiota	56:73	The gut microbiota is considered as our other "brain" and is implicated in several regulation of physiological metabolisms.
28756077	1	31	theme	brain	103:107	arg1	"					108:108	our other "brain"	92:108	our other "brain"	92:108	The gut microbiota is considered as our other "brain" and is implicated in several regulation of physiological metabolisms.
28756077	1	31	theme	brain	103:107	arg1	microbiota					64:73	The gut microbiota	56:73	The gut microbiota	56:73	The gut microbiota is considered as our other "brain" and is implicated in several regulation of physiological metabolisms.
28756077	2	32	theme	TMAO	205:208	arg1	level					196:200	The circulating level	180:200	The circulating level of TMAO, a metabolite of the gut microbiota,	180:245	The circulating level of TMAO, a metabolite of the gut microbiota, is directly correlated to the occurrence of cardiovascular events.
28756077	3	33	theme	protective	329:338	arg1	acids					319:323	Bile acids	314:323	Bile acids	314:323	Bile acids are protective metabolites against cardiovascular diseases through their anti-inflammatory and anti-atherogenic effects.
28756077	3	33	theme	protective	329:338	arg1	metabolites					340:350	protective metabolites	329:350	protective metabolites against cardiovascular diseases	329:382	Bile acids are protective metabolites against cardiovascular diseases through their anti-inflammatory and anti-atherogenic effects.
28484097	6	0	with	porous	1161:1166	arg1	behavior					1205:1212	tunable swelling behavior	1188:1212	tunable swelling behavior	1188:1212	We observed that the matrigel-collagen hydrogel was porous and degradable with tunable swelling behavior.
28484097	1	1	from	matrix	302:307	arg1	environment					337:347	a three-dimensional (3D) environment	312:347	a three-dimensional (3D) environment	312:347	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	3	2	theme	poor	526:529	arg1	behavior					542:549	poor mechanical behavior	526:549	poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets	526:634	Unfortunately, this material has poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets.
28484097	4	3	contain	contains	691:698	arg2	proteoglycans					783:795	sulfated proteoglycans	774:795	sulfated proteoglycans	774:795	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	3	contain	contains	691:698	arg2	collagen					761:768	type IV collagen	753:768	type IV collagen	753:768	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	3	contain	contains	691:698	arg1	matrigel					675:682	matrigel	675:682	matrigel	675:682	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	3	contain	contains	691:698	arg2	components					734:743	other heart valve-relevant matrix components	700:743	other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans	700:795	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	4	theme	physiological	846:858	arg1	milieu					860:865	an appropriate physiological milieu	831:865	an appropriate physiological milieu for in vitro valve interstitial cell culture	831:910	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	6	5	theme	swelling	1196:1203	arg1	behavior					1205:1212	tunable swelling behavior	1188:1212	tunable swelling behavior	1188:1212	We observed that the matrigel-collagen hydrogel was porous and degradable with tunable swelling behavior.
28484097	9	6	theme	matrigel-collagen	1751:1767	arg1	hydrogel					1769:1776	the matrigel-collagen hydrogel	1747:1776	the matrigel-collagen hydrogel	1747:1776	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	9	7	theme	composite	1617:1625	arg1	hydrogel					1627:1634	the composite hydrogel	1613:1634	the composite hydrogel	1613:1634	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	9	7	theme	composite	1617:1625	arg1	amenable					1645:1652	amenable	1645:1652	amenable	1645:1652	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	1	8	theme	dynamic	373:379	arg1	forces					392:397	multiple dynamic mechanical forces	364:397	multiple dynamic mechanical forces	364:397	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	7	9	dep	in	1420:1421	arg1	vitro					1423:1427	vitro	1423:1427	vitro	1423:1427	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	4	10	theme	interstitial	886:897	arg1	culture					904:910	in vitro valve interstitial cell culture	871:910	in vitro valve interstitial cell culture	871:910	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	1	11	dep	in	232:233	arg1	vivo					235:238	vivo	235:238	vivo	235:238	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	4	12	theme	other	700:704	arg1	collagen					761:768	type IV collagen	753:768	type IV collagen	753:768	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	12	theme	other	700:704	arg1	components					734:743	other heart valve-relevant matrix components	700:743	other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans	700:795	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	12	theme	other	700:704	arg1	proteoglycans					783:795	sulfated proteoglycans	774:795	sulfated proteoglycans	774:795	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	5	13	theme	mechanical	1051:1060	arg1	properties					1083:1092	their physical, mechanical and biocompatibility properties	1035:1092	their physical, mechanical and biocompatibility properties	1035:1092	Different semi-interpenetrating mixtures of collagen type I and matrigel were prepared and a thorough characterization of their physical, mechanical and biocompatibility properties was performed.
28484097	3	14	theme	inherent	561:568	arg1	propensity					570:579	an inherent propensity	558:579	an inherent propensity to compact significantly, unlike native valve leaflets	558:634	Unfortunately, this material has poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets.
28484097	5	15	theme	Different	913:921	arg1	mixtures					945:952	Different semi-interpenetrating mixtures	913:952	Different semi-interpenetrating mixtures of collagen type I and matrigel	913:984	Different semi-interpenetrating mixtures of collagen type I and matrigel were prepared and a thorough characterization of their physical, mechanical and biocompatibility properties was performed.
28484097	9	16	theme	hydrogel	1769:1776	arg1	use					1740:1742	the potential use	1726:1742	the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro	1726:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	4	17	theme	valve-relevant	712:725	arg1	collagen					761:768	type IV collagen	753:768	type IV collagen	753:768	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	17	theme	valve-relevant	712:725	arg1	components					734:743	other heart valve-relevant matrix components	700:743	other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans	700:795	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	17	theme	valve-relevant	712:725	arg1	proteoglycans					783:795	sulfated proteoglycans	774:795	sulfated proteoglycans	774:795	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	5	18	theme	biocompatibility	1066:1081	arg1	properties					1083:1092	their physical, mechanical and biocompatibility properties	1035:1092	their physical, mechanical and biocompatibility properties	1035:1092	Different semi-interpenetrating mixtures of collagen type I and matrigel were prepared and a thorough characterization of their physical, mechanical and biocompatibility properties was performed.
28484097	8	19	theme	cell	1538:1541	arg1	phenotype					1543:1551	cell phenotype	1538:1551	cell phenotype	1538:1551	We showed that cells cultured in the composite hydrogel had comparable viability, proliferation and cell phenotype as compared with those in a collagen only gel.
28484097	7	20	theme	matrix	1401:1406	arg1	network					1408:1414	a more enriched extracellular matrix network	1371:1414	a more enriched extracellular matrix network for in vitro culture	1371:1435	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	4	21	theme	matrigel	675:682	arg1	incorporation					658:670	incorporation	658:670	incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen	658:815	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	1	22	theme	three-dimensional	314:330	arg1	environment					337:347	a three-dimensional (3D) environment	312:347	a three-dimensional (3D) environment	312:347	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	2	23	theme	valve	471:475	arg1	cells					477:481	culture valve cells	463:481	culture valve cells	463:481	Type I collagen is typically the most common 3D matrix used to culture valve cells in vitro.
28484097	0	24	theme	valve	85:89	arg1	culture					109:115	dynamic valve interstitial cell culture	77:115	dynamic valve interstitial cell culture	77:115	Fabrication of a matrigel-collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture.
28484097	1	25	theme	mechanisms	167:176	arg1	study					122:126	The study	118:126	The study of heart valve homeostatic and disease mechanisms	118:176	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	0	26	theme	cell	104:107	arg1	culture					109:115	dynamic valve interstitial cell culture	77:115	dynamic valve interstitial cell culture	77:115	Fabrication of a matrigel-collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture.
28484097	5	27	theme	matrigel	977:984	arg1	mixtures					945:952	Different semi-interpenetrating mixtures	913:952	Different semi-interpenetrating mixtures of collagen type I and matrigel	913:984	Different semi-interpenetrating mixtures of collagen type I and matrigel were prepared and a thorough characterization of their physical, mechanical and biocompatibility properties was performed.
28484097	9	28	theme	dynamic	1818:1824	arg1	culture					1837:1843	the dynamic mechanical culture	1814:1843	the dynamic mechanical culture of valve interstitial cells in vitro	1814:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	7	29	theme	extracellular	1387:1399	arg1	network					1408:1414	a more enriched extracellular matrix network	1371:1414	a more enriched extracellular matrix network for in vitro culture	1371:1435	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	2	30	theme	common	438:443	arg1	matrix					448:453	the most common 3D matrix	429:453	the most common 3D matrix used to culture valve cells in vitro	429:490	Type I collagen is typically the most common 3D matrix used to culture valve cells in vitro.
28484097	2	30	theme	common	438:443	arg1	collagen					407:414	Type I collagen	400:414	Type I collagen	400:414	Type I collagen is typically the most common 3D matrix used to culture valve cells in vitro.
28484097	8	31	theme	collagen	1581:1588	arg1	gel					1595:1597	a collagen only gel	1579:1597	a collagen only gel	1579:1597	We showed that cells cultured in the composite hydrogel had comparable viability, proliferation and cell phenotype as compared with those in a collagen only gel.
28484097	5	32	theme	collagen	957:964	arg1	type					966:969	collagen type I	957:971	collagen type I	957:971	Different semi-interpenetrating mixtures of collagen type I and matrigel were prepared and a thorough characterization of their physical, mechanical and biocompatibility properties was performed.
28484097	9	33	theme	valve	1848:1852	arg1	cells					1867:1871	valve interstitial cells	1848:1871	valve interstitial cells in vitro	1848:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	9	34	theme	cells	1867:1871	arg1	culture					1837:1843	the dynamic mechanical culture	1814:1843	the dynamic mechanical culture of valve interstitial cells in vitro	1814:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	4	35	theme	I	806:806	arg1	collagen					808:815	type I collagen	801:815	type I collagen	801:815	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	0	36	theme	semi-interpenetrating	35:55	arg1	scaffold					57:64	a matrigel-collagen semi-interpenetrating scaffold	15:64	a matrigel-collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture	15:115	Fabrication of a matrigel-collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture.
28484097	1	37	theme	extracellular	288:300	arg1	matrix					302:307	the extracellular matrix	284:307	the extracellular matrix in a three-dimensional (3D) environment	284:347	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	4	38	dep	in	871:872	arg1	vitro					874:878	vitro	874:878	vitro	874:878	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	7	39	theme	interstitial	1347:1358	arg1	cells					1360:1364	the cultured valve interstitial cells	1328:1364	the cultured valve interstitial cells	1328:1364	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	3	40	theme	valve	621:625	arg1	leaflets					627:634	native valve leaflets	614:634	native valve leaflets	614:634	Unfortunately, this material has poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets.
28484097	9	41	theme	in	1657:1658	arg1	culture					1684:1690	in vitro cyclic stretching culture	1657:1690	in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro	1657:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	7	42	theme	cultured	1332:1339	arg1	cells					1360:1364	the cultured valve interstitial cells	1328:1364	the cultured valve interstitial cells	1328:1364	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	3	43	contain	has	522:524	arg2	behavior					542:549	poor mechanical behavior	526:549	poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets	526:634	Unfortunately, this material has poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets.
28484097	3	43	contain	has	522:524	arg1	material					513:520	this material	508:520	this material	508:520	Unfortunately, this material has poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets.
28484097	9	44	theme	cyclic	1666:1671	arg1	culture					1684:1690	in vitro cyclic stretching culture	1657:1690	in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro	1657:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	9	45	theme	dimensional	1789:1799	arg1	scaffold					1801:1808	a three dimensional scaffold	1781:1808	a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro	1781:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	7	46	theme	composite	1290:1298	arg1	hydrogel					1300:1307	the composite hydrogel	1286:1307	the composite hydrogel	1286:1307	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	8	47	contain	had	1494:1496	arg1	cells					1453:1457	cells	1453:1457	cells cultured in the composite hydrogel	1453:1492	We showed that cells cultured in the composite hydrogel had comparable viability, proliferation and cell phenotype as compared with those in a collagen only gel.
28484097	8	47	contain	had	1494:1496	arg2	phenotype					1543:1551	cell phenotype	1538:1551	cell phenotype	1538:1551	We showed that cells cultured in the composite hydrogel had comparable viability, proliferation and cell phenotype as compared with those in a collagen only gel.
28484097	8	47	contain	had	1494:1496	arg2	proliferation					1520:1532	proliferation	1520:1532	proliferation	1520:1532	We showed that cells cultured in the composite hydrogel had comparable viability, proliferation and cell phenotype as compared with those in a collagen only gel.
28484097	8	47	contain	had	1494:1496	arg2	viability					1509:1517	comparable viability	1498:1517	comparable viability	1498:1517	We showed that cells cultured in the composite hydrogel had comparable viability, proliferation and cell phenotype as compared with those in a collagen only gel.
28484097	1	48	theme	valve	254:258	arg1	cells					260:264	valve cells	254:264	valve cells	254:264	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	4	49	theme	in	871:872	arg1	culture					904:910	in vitro valve interstitial cell culture	871:910	in vitro valve interstitial cell culture	871:910	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	9	50	dep	in	1657:1658	arg1	vitro					1660:1664	vitro	1660:1664	vitro	1660:1664	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	7	51	theme	mechanical	1263:1272	arg1	behavior					1274:1281	the mechanical behavior	1259:1281	the mechanical behavior of the composite hydrogel	1259:1307	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	3	52	theme	due	551:553	arg1	behavior					542:549	poor mechanical behavior	526:549	poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets	526:634	Unfortunately, this material has poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets.
28484097	4	53	theme	appropriate	834:844	arg1	milieu					860:865	an appropriate physiological milieu	831:865	an appropriate physiological milieu for in vitro valve interstitial cell culture	831:910	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	6	54	theme	tunable	1188:1194	arg1	behavior					1205:1212	tunable swelling behavior	1188:1212	tunable swelling behavior	1188:1212	We observed that the matrigel-collagen hydrogel was porous and degradable with tunable swelling behavior.
28484097	3	55	theme	mechanical	531:540	arg1	behavior					542:549	poor mechanical behavior	526:549	poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets	526:634	Unfortunately, this material has poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets.
28484097	9	56	theme	potential	1730:1738	arg1	use					1740:1742	the potential use	1726:1742	the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro	1726:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	8	57	theme	comparable	1498:1507	arg1	viability					1509:1517	comparable viability	1498:1517	comparable viability	1498:1517	We showed that cells cultured in the composite hydrogel had comparable viability, proliferation and cell phenotype as compared with those in a collagen only gel.
28484097	1	58	theme	mechanical	381:390	arg1	forces					392:397	multiple dynamic mechanical forces	364:397	multiple dynamic mechanical forces	364:397	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	4	59	theme	valve	880:884	arg1	culture					904:910	in vitro valve interstitial cell culture	871:910	in vitro valve interstitial cell culture	871:910	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	5	60	theme	semi-interpenetrating	923:943	arg1	mixtures					945:952	Different semi-interpenetrating mixtures	913:952	Different semi-interpenetrating mixtures of collagen type I and matrigel	913:984	Different semi-interpenetrating mixtures of collagen type I and matrigel were prepared and a thorough characterization of their physical, mechanical and biocompatibility properties was performed.
28484097	4	61	theme	cell	899:902	arg1	culture					904:910	in vitro valve interstitial cell culture	871:910	in vitro valve interstitial cell culture	871:910	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	7	62	theme	in	1420:1421	arg1	culture					1429:1435	in vitro culture	1420:1435	in vitro culture	1420:1435	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	4	63	theme	heart	706:710	arg1	collagen					761:768	type IV collagen	753:768	type IV collagen	753:768	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	63	theme	heart	706:710	arg1	components					734:743	other heart valve-relevant matrix components	700:743	other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans	700:795	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	63	theme	heart	706:710	arg1	proteoglycans					783:795	sulfated proteoglycans	774:795	sulfated proteoglycans	774:795	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	0	64	theme	dynamic	77:83	arg1	culture					109:115	dynamic valve interstitial cell culture	77:115	dynamic valve interstitial cell culture	77:115	Fabrication of a matrigel-collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture.
28484097	5	65	theme	properties	1083:1092	arg1	characterization					1015:1030	a thorough characterization	1004:1030	a thorough characterization of their physical, mechanical and biocompatibility properties	1004:1092	Different semi-interpenetrating mixtures of collagen type I and matrigel were prepared and a thorough characterization of their physical, mechanical and biocompatibility properties was performed.
28484097	1	66	theme	homeostatic	143:153	arg1	study					122:126	The study	118:126	The study of heart valve homeostatic and disease mechanisms	118:176	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	0	67	theme	interstitial	91:102	arg1	culture					109:115	dynamic valve interstitial cell culture	77:115	dynamic valve interstitial cell culture	77:115	Fabrication of a matrigel-collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture.
28484097	1	68	theme	disease	159:165	arg1	mechanisms					167:176	heart valve homeostatic and disease mechanisms	131:176	mechanisms	167:176	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	2	69	theme	culture	463:469	arg1	cells					477:481	culture valve cells	463:481	culture valve cells	463:481	Type I collagen is typically the most common 3D matrix used to culture valve cells in vitro.
28484097	0	70	from	use	70:72	arg1	culture					109:115	dynamic valve interstitial cell culture	77:115	dynamic valve interstitial cell culture	77:115	Fabrication of a matrigel-collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture.
28484097	4	71	theme	type	753:756	arg1	collagen					761:768	type IV collagen	753:768	type IV collagen	753:768	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	7	72	theme	matrigel	1232:1239	arg1	Incorporation					1215:1227	Incorporation	1215:1227	Incorporation of matrigel	1215:1239	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	7	73	theme	enriched	1378:1385	arg1	network					1408:1414	a more enriched extracellular matrix network	1371:1414	a more enriched extracellular matrix network for in vitro culture	1371:1435	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	9	74	theme	mechanical	1826:1835	arg1	culture					1837:1843	the dynamic mechanical culture	1814:1843	the dynamic mechanical culture of valve interstitial cells in vitro	1814:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	8	75	theme	only	1590:1593	arg1	gel					1595:1597	a collagen only gel	1579:1597	a collagen only gel	1579:1597	We showed that cells cultured in the composite hydrogel had comparable viability, proliferation and cell phenotype as compared with those in a collagen only gel.
28484097	9	76	dep	h.	1699:1700	arg1	report					1714:1719	report	1714:1719	report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro	1714:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	1	77	theme	multiple	364:371	arg1	forces					392:397	multiple dynamic mechanical forces	364:397	multiple dynamic mechanical forces	364:397	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	4	78	theme	matrix	727:732	arg1	collagen					761:768	type IV collagen	753:768	type IV collagen	753:768	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	78	theme	matrix	727:732	arg1	components					734:743	other heart valve-relevant matrix components	700:743	other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans	700:795	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	4	78	theme	matrix	727:732	arg1	proteoglycans					783:795	sulfated proteoglycans	774:795	sulfated proteoglycans	774:795	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	0	79	theme	matrigel-collagen	17:33	arg1	scaffold					57:64	a matrigel-collagen semi-interpenetrating scaffold	15:64	a matrigel-collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture	15:115	Fabrication of a matrigel-collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture.
28484097	5	80	theme	thorough	1006:1013	arg1	characterization					1015:1030	a thorough characterization	1004:1030	a thorough characterization of their physical, mechanical and biocompatibility properties	1004:1092	Different semi-interpenetrating mixtures of collagen type I and matrigel were prepared and a thorough characterization of their physical, mechanical and biocompatibility properties was performed.
28484097	5	81	theme	type	966:969	arg1	mixtures					945:952	Different semi-interpenetrating mixtures	913:952	Different semi-interpenetrating mixtures of collagen type I and matrigel	913:984	Different semi-interpenetrating mixtures of collagen type I and matrigel were prepared and a thorough characterization of their physical, mechanical and biocompatibility properties was performed.
28484097	9	82	theme	interstitial	1854:1865	arg1	cells					1867:1871	valve interstitial cells	1848:1871	valve interstitial cells in vitro	1848:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	1	83	dep	three-dimensional	314:330	arg1	3D					333:334	3D	333:334	3D	333:334	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	6	84	theme	matrigel-collagen	1130:1146	arg1	porous					1161:1166	porous	1161:1166	porous	1161:1166	We observed that the matrigel-collagen hydrogel was porous and degradable with tunable swelling behavior.
28484097	6	84	theme	matrigel-collagen	1130:1146	arg1	hydrogel					1148:1155	the matrigel-collagen hydrogel	1126:1155	the matrigel-collagen hydrogel	1126:1155	We observed that the matrigel-collagen hydrogel was porous and degradable with tunable swelling behavior.
28484097	0	85	theme	scaffold	57:64	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of a matrigel-collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture	0:115	Fabrication of a matrigel-collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture.
28484097	7	86	theme	valve	1341:1345	arg1	cells					1360:1364	the cultured valve interstitial cells	1328:1364	the cultured valve interstitial cells	1328:1364	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	3	87	theme	native	614:619	arg1	leaflets					627:634	native valve leaflets	614:634	native valve leaflets	614:634	Unfortunately, this material has poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets.
28484097	4	88	theme	type	801:804	arg1	collagen					808:815	type I collagen	801:815	type I collagen	801:815	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	2	89	theme	Type	400:403	arg1	matrix					448:453	the most common 3D matrix	429:453	the most common 3D matrix used to culture valve cells in vitro	429:490	Type I collagen is typically the most common 3D matrix used to culture valve cells in vitro.
28484097	2	89	theme	Type	400:403	arg1	collagen					407:414	Type I collagen	400:414	Type I collagen	400:414	Type I collagen is typically the most common 3D matrix used to culture valve cells in vitro.
28484097	5	90	theme	physical	1041:1048	arg1	properties					1083:1092	their physical, mechanical and biocompatibility properties	1035:1092	their physical, mechanical and biocompatibility properties	1035:1092	Different semi-interpenetrating mixtures of collagen type I and matrigel were prepared and a thorough characterization of their physical, mechanical and biocompatibility properties was performed.
28484097	2	91	theme	3D	445:446	arg1	matrix					448:453	the most common 3D matrix	429:453	the most common 3D matrix used to culture valve cells in vitro	429:490	Type I collagen is typically the most common 3D matrix used to culture valve cells in vitro.
28484097	2	91	theme	3D	445:446	arg1	collagen					407:414	Type I collagen	400:414	Type I collagen	400:414	Type I collagen is typically the most common 3D matrix used to culture valve cells in vitro.
28484097	4	92	theme	sulfated	774:781	arg1	proteoglycans					783:795	sulfated proteoglycans	774:795	sulfated proteoglycans	774:795	We hypothesized that incorporation of matrigel, which contains other heart valve-relevant matrix components such as type IV collagen and sulfated proteoglycans, to type I collagen would provide an appropriate physiological milieu for in vitro valve interstitial cell culture.
28484097	1	93	theme	in	232:233	arg1	milieu					240:245	the in vivo milieu	228:245	the in vivo milieu	228:245	The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three-dimensional (3D) environment and experience multiple dynamic mechanical forces.
28484097	9	94	theme	stretching	1673:1682	arg1	culture					1684:1690	in vitro cyclic stretching culture	1657:1690	in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro	1657:1880	Importantly, the composite hydrogel was also amenable to in vitro cyclic stretching culture for 48 h. Overall, we report here the potential use of the matrigel-collagen hydrogel as a three dimensional scaffold for the dynamic mechanical culture of valve interstitial cells in vitro.
28484097	7	95	theme	hydrogel	1300:1307	arg1	behavior					1274:1281	the mechanical behavior	1259:1281	the mechanical behavior of the composite hydrogel	1259:1307	Incorporation of matrigel not only enhanced the mechanical behavior of the composite hydrogel but also presented the cultured valve interstitial cells with a more enriched extracellular matrix network for in vitro culture.
28484097	8	96	theme	composite	1475:1483	arg1	hydrogel					1485:1492	the composite hydrogel	1471:1492	the composite hydrogel	1471:1492	We showed that cells cultured in the composite hydrogel had comparable viability, proliferation and cell phenotype as compared with those in a collagen only gel.
26204529	10	0	theme	vein/vascular	1862:1874	arg1	cells					1888:1892	human, umbilical vein/vascular endothelial cells	1845:1892	cells	1888:1892	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	10	0	theme	vein/vascular	1862:1874	arg1	cells					1904:1908	EA.HY926 cells	1895:1908	EA.HY926 cells	1895:1908	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	10	1	dep	poly	1737:1740	arg1	peptide					1801:1807	the His/Gly-tagged RGD peptide	1778:1807	the His/Gly-tagged RGD peptide	1778:1807	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	12	2	theme	scaffold	2086:2093	arg1	biomaterial					2116:2126	a promising biomaterial	2104:2126	a promising biomaterial for future prosthetic vascular grafts	2104:2164	We propose that the metabolically degradable polymeric scaffold bTEBV is a promising biomaterial for future prosthetic vascular grafts.
26204529	12	2	theme	scaffold	2086:2093	arg1	bTEBV					2095:2099	the metabolically degradable polymeric scaffold bTEBV	2047:2099	the metabolically degradable polymeric scaffold bTEBV	2047:2099	We propose that the metabolically degradable polymeric scaffold bTEBV is a promising biomaterial for future prosthetic vascular grafts.
26204529	8	3	theme	4-week	1523:1528	arg1	experiments					1545:1555	4-week pulsatile flow experiments	1523:1555	4-week pulsatile flow experiments	1523:1555	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	11	4	from	effect	1958:1963	arg1	clotting					1993:2000	the clotting	1989:2000	the clotting of human plasma	1989:2016	No significant effect of the polyP samples on the clotting of human plasma is measured.
26204529	8	5	theme	size	1420:1423	arg1	mm					1427:1428	an outer size 6 mm	1411:1428	an outer size 6 mm	1411:1428	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	12	6	theme	prosthetic	2139:2148	arg1	grafts					2159:2164	future prosthetic vascular grafts	2132:2164	future prosthetic vascular grafts	2132:2164	We propose that the metabolically degradable polymeric scaffold bTEBV is a promising biomaterial for future prosthetic vascular grafts.
26204529	8	7	theme	artificial	1371:1380	arg1	grafts					1391:1396	the artificial vascular grafts	1367:1396	the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively)	1367:1493	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	8	8	theme	flow	1540:1543	arg1	experiments					1545:1555	4-week pulsatile flow experiments	1523:1555	4-week pulsatile flow experiments	1523:1555	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	10	9	theme	EA.HY926	1895:1902	arg1	cells					1888:1892	human, umbilical vein/vascular endothelial cells	1845:1892	cells	1888:1892	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	10	9	theme	EA.HY926	1895:1902	arg1	cells					1904:1908	EA.HY926 cells	1895:1908	EA.HY926 cells	1895:1908	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	7	10	theme	biopolymer	1187:1196	arg1	material					1198:1205	the biopolymer material	1183:1205	the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica)	1183:1244	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	10	11	theme	poly	1737:1740	arg1	Incorporation					1707:1719	Incorporation	1707:1719	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide,	1707:1808	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	3	12	theme	inorganic	503:511	arg1	polyphosphate					513:525	inorganic polyphosphate	503:525	inorganic polyphosphate (polyP)	503:533	Into this biologically inert scaffold two biofunctionally active biopolymers are embedded, inorganic polyphosphate (polyP) and silica, as well as gelatin which exposes the cell recognition signal, Arg-Gly-Asp (RGD).
26204529	3	12	theme	inorganic	503:511	arg1	polyP					528:532	polyP	528:532	polyP	528:532	Into this biologically inert scaffold two biofunctionally active biopolymers are embedded, inorganic polyphosphate (polyP) and silica, as well as gelatin which exposes the cell recognition signal, Arg-Gly-Asp (RGD).
26204529	1	13	theme	biomimetic	164:173	arg1	bTEBV					208:212	bTEBV	208:212	bTEBV	208:212	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	1	13	theme	biomimetic	164:173	arg1	vessels					199:205	biomimetic tissue-engineered blood vessels	164:205	biomimetic tissue-engineered blood vessels (bTEBV)	164:213	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	7	14	theme	2+	1220:1221	arg1	O-CMC-polyP-silica					1226:1243	O-CMC-polyP-silica	1226:1243	O-CMC-polyP-silica	1226:1243	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	7	14	theme	2+	1220:1221	arg1	-N					1223:1224	alginate-Ca(2+)-N	1208:1224	alginate-Ca(2+)-N	1208:1224	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	10	15	theme	cells	1888:1892	arg1	adhesion					1833:1840	the adhesion	1829:1840	the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel	1829:1940	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	12	16	theme	degradable	2065:2074	arg1	biomaterial					2116:2126	a promising biomaterial	2104:2126	a promising biomaterial for future prosthetic vascular grafts	2104:2164	We propose that the metabolically degradable polymeric scaffold bTEBV is a promising biomaterial for future prosthetic vascular grafts.
26204529	12	16	theme	degradable	2065:2074	arg1	bTEBV					2095:2099	the metabolically degradable polymeric scaffold bTEBV	2047:2099	the metabolically degradable polymeric scaffold bTEBV	2047:2099	We propose that the metabolically degradable polymeric scaffold bTEBV is a promising biomaterial for future prosthetic vascular grafts.
26204529	8	17	theme	alternating	1563:1573	arg1	pressure					1575:1582	an alternating pressure	1560:1582	an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg)	1560:1628	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	1	18	theme	blood	193:197	arg1	bTEBV					208:212	bTEBV	208:212	bTEBV	208:212	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	1	18	theme	blood	193:197	arg1	vessels					199:205	biomimetic tissue-engineered blood vessels	164:205	biomimetic tissue-engineered blood vessels (bTEBV)	164:213	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	1	19	with	fabrication	85:95	arg1	composition					231:241	a modular composition	221:241	a modular composition	221:241	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	7	20	theme	mechanical	1158:1167	arg1	properties					1169:1178	The mechanical properties	1154:1178	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica)	1154:1244	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	8	21	theme	diameter	1455:1462	arg1	mm					1466:1467	an inner diameter 4 mm and 0.8 mm	1446:1478	mm	1466:1467	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	10	22	theme	hydrogel	1933:1940	arg1	surface					1918:1924	the surface	1914:1924	the surface of the hydrogel	1914:1940	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	5	23	theme	hardening	877:885	arg1	solution					887:894	a hardening solution	875:894	a hardening solution	875:894	The bTEBV are formed by pressing the hydrogel through an extruder into a hardening solution, containing Ca(2+).
26204529	7	24	dep	material	1198:1205	arg1	O-CMC-polyP-silica					1226:1243	O-CMC-polyP-silica	1226:1243	O-CMC-polyP-silica	1226:1243	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	7	24	dep	material	1198:1205	arg1	-N					1223:1224	alginate-Ca(2+)-N	1208:1224	alginate-Ca(2+)-N	1208:1224	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	1	25	theme	small	127:131	arg1	vessels					148:154	artificial small diameter blood vessels	116:154	artificial small diameter blood vessels	116:154	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	1	26	theme	blood	142:146	arg1	vessels					148:154	artificial small diameter blood vessels	116:154	artificial small diameter blood vessels	116:154	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	7	27	theme	incubation	1314:1323	arg1	period					1325:1330	a short incubation period	1306:1330	a short incubation period in CaCl2 solution	1306:1348	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	11	28	theme	plasma	2011:2016	arg1	clotting					1993:2000	the clotting	1989:2000	the clotting of human plasma	1989:2016	No significant effect of the polyP samples on the clotting of human plasma is measured.
26204529	4	29	dep	polyP	765:769	arg1	O-CMC-polyP					790:800	O-CMC-polyP	790:800	O-CMC-polyP	790:800	These materials can be hardened by exposure to Ca(2+) through formation of Ca(2+) bridges between the polyanions, alginate, N,O-CMC, and polyP (alginate-Ca(2+)-N,O-CMC-polyP).
26204529	4	29	dep	polyP	765:769	arg1	-N					787:788	alginate-Ca(2+)-N	772:788	alginate-Ca(2+)-N	772:788	These materials can be hardened by exposure to Ca(2+) through formation of Ca(2+) bridges between the polyanions, alginate, N,O-CMC, and polyP (alginate-Ca(2+)-N,O-CMC-polyP).
26204529	10	30	theme	RGD	1797:1799	arg1	peptide					1801:1807	the His/Gly-tagged RGD peptide	1778:1807	the His/Gly-tagged RGD peptide	1778:1807	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	6	31	theme	endothelial	1113:1123	arg1	cells					1125:1129	endothelial cells	1113:1129	endothelial cells	1113:1129	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	11	32	theme	samples	1978:1984	arg1	effect					1958:1963	No significant effect	1943:1963	No significant effect of the polyP samples on the clotting of human plasma	1943:2016	No significant effect of the polyP samples on the clotting of human plasma is measured.
26204529	0	33	theme	Modular	0:6	arg1	Grafts					32:37	Modular Small Diameter Vascular Grafts	0:37	Modular Small Diameter Vascular Grafts with Bioactive Functionalities	0:68	Modular Small Diameter Vascular Grafts with Bioactive Functionalities.
26204529	8	34	with	bTEBVs	1399:1404	arg1	mm					1466:1467	an inner diameter 4 mm and 0.8 mm	1446:1478	mm	1466:1467	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	8	34	with	bTEBVs	1399:1404	arg1	mm					1427:1428	an outer size 6 mm	1411:1428	an outer size 6 mm	1411:1428	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	8	34	with	bTEBVs	1399:1404	arg1	mm					1438:1439	1.8 mm	1434:1439	1.8 mm	1434:1439	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	0	35	theme	Diameter	14:21	arg1	Grafts					32:37	Modular Small Diameter Vascular Grafts	0:37	Modular Small Diameter Vascular Grafts with Bioactive Functionalities	0:68	Modular Small Diameter Vascular Grafts with Bioactive Functionalities.
26204529	12	36	theme	vascular	2150:2157	arg1	grafts					2159:2164	future prosthetic vascular grafts	2132:2164	future prosthetic vascular grafts	2132:2164	We propose that the metabolically degradable polymeric scaffold bTEBV is a promising biomaterial for future prosthetic vascular grafts.
26204529	1	37	theme	novel	102:106	arg1	type					108:111	a novel type	100:111	a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV),	100:214	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	5	38	contain	containing	897:906	arg2	2+					911:912	2+	911:912	2+	911:912	The bTEBV are formed by pressing the hydrogel through an extruder into a hardening solution, containing Ca(2+).
26204529	5	38	contain	containing	897:906	arg1	solution					887:894	a hardening solution	875:894	a hardening solution	875:894	The bTEBV are formed by pressing the hydrogel through an extruder into a hardening solution, containing Ca(2+).
26204529	5	38	contain	containing	897:906	arg2	Ca					908:909	Ca	908:909	Ca(2+)	908:913	The bTEBV are formed by pressing the hydrogel through an extruder into a hardening solution, containing Ca(2+).
26204529	2	39	theme	O-carboxymethyl	376:390	arg1	chitosan					392:399	O-carboxymethyl chitosan	376:399	O-carboxymethyl chitosan (N,O-CMC)	376:409	They are composed of a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC).
26204529	2	39	theme	O-carboxymethyl	376:390	arg1	chitosan					364:371	a modified chitosan	353:371	a modified chitosan	353:371	They are composed of a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC).
26204529	3	40	theme	recognition	589:599	arg1	signal					601:606	the cell recognition signal	580:606	the cell recognition signal	580:606	Into this biologically inert scaffold two biofunctionally active biopolymers are embedded, inorganic polyphosphate (polyP) and silica, as well as gelatin which exposes the cell recognition signal, Arg-Gly-Asp (RGD).
26204529	3	40	theme	recognition	589:599	arg1	Arg-Gly-Asp					609:619	Arg-Gly-Asp	609:619	Arg-Gly-Asp (RGD)	609:625	Into this biologically inert scaffold two biofunctionally active biopolymers are embedded, inorganic polyphosphate (polyP) and silica, as well as gelatin which exposes the cell recognition signal, Arg-Gly-Asp (RGD).
26204529	0	41	theme	Bioactive	44:52	arg1	Functionalities					54:68	Bioactive Functionalities	44:68	Bioactive Functionalities	44:68	Modular Small Diameter Vascular Grafts with Bioactive Functionalities.
26204529	3	42	theme	inert	435:439	arg1	scaffold					441:448	this biologically inert scaffold	417:448	this biologically inert scaffold	417:448	Into this biologically inert scaffold two biofunctionally active biopolymers are embedded, inorganic polyphosphate (polyP) and silica, as well as gelatin which exposes the cell recognition signal, Arg-Gly-Asp (RGD).
26204529	8	43	theme	vascular	1382:1389	arg1	grafts					1391:1396	the artificial vascular grafts	1367:1396	the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively)	1367:1493	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	12	44	theme	future	2132:2137	arg1	grafts					2159:2164	future prosthetic vascular grafts	2132:2164	future prosthetic vascular grafts	2132:2164	We propose that the metabolically degradable polymeric scaffold bTEBV is a promising biomaterial for future prosthetic vascular grafts.
26204529	12	45	theme	polymeric	2076:2084	arg1	biomaterial					2116:2126	a promising biomaterial	2104:2126	a promising biomaterial for future prosthetic vascular grafts	2104:2164	We propose that the metabolically degradable polymeric scaffold bTEBV is a promising biomaterial for future prosthetic vascular grafts.
26204529	12	45	theme	polymeric	2076:2084	arg1	bTEBV					2095:2099	the metabolically degradable polymeric scaffold bTEBV	2047:2099	the metabolically degradable polymeric scaffold bTEBV	2047:2099	We propose that the metabolically degradable polymeric scaffold bTEBV is a promising biomaterial for future prosthetic vascular grafts.
26204529	8	46	theme	pulsatile	1530:1538	arg1	experiments					1545:1555	4-week pulsatile flow experiments	1523:1555	4-week pulsatile flow experiments	1523:1555	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	0	47	with	Grafts	32:37	arg1	Functionalities					54:68	Bioactive Functionalities	44:68	Bioactive Functionalities	44:68	Modular Small Diameter Vascular Grafts with Bioactive Functionalities.
26204529	3	48	theme	active	470:475	arg1	biopolymers					477:487	two biofunctionally active biopolymers	450:487	two biofunctionally active biopolymers	450:487	Into this biologically inert scaffold two biofunctionally active biopolymers are embedded, inorganic polyphosphate (polyP) and silica, as well as gelatin which exposes the cell recognition signal, Arg-Gly-Asp (RGD).
26204529	10	49	theme	umbilical	1852:1860	arg1	cells					1888:1892	human, umbilical vein/vascular endothelial cells	1845:1892	cells	1888:1892	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	10	49	theme	umbilical	1852:1860	arg1	cells					1904:1908	EA.HY926 cells	1895:1908	EA.HY926 cells	1895:1908	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	6	50	theme	RGD	1053:1055	arg1	peptide					1038:1044	a His/Gly-tagged RGD peptide	1017:1044	a His/Gly-tagged RGD peptide (three RGD units)	1017:1062	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	6	50	theme	RGD	1053:1055	arg1	units					1057:1061	three RGD units	1047:1061	three RGD units	1047:1061	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	2	51	theme	hydrogel	267:274	arg1	scaffold					276:283	a hydrogel scaffold	265:283	a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC)	265:409	They are composed of a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC).
26204529	8	52	theme	outer	1414:1418	arg1	mm					1427:1428	an outer size 6 mm	1411:1428	an outer size 6 mm	1411:1428	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	10	53	theme	human	1845:1849	arg1	cells					1888:1892	human, umbilical vein/vascular endothelial cells	1845:1892	cells	1888:1892	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	10	53	theme	human	1845:1849	arg1	cells					1904:1908	EA.HY926 cells	1895:1908	EA.HY926 cells	1895:1908	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	7	54	theme	alginate-Ca	1208:1218	arg1	O-CMC-polyP-silica					1226:1243	O-CMC-polyP-silica	1226:1243	O-CMC-polyP-silica	1226:1243	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	7	54	theme	alginate-Ca	1208:1218	arg1	-N					1223:1224	alginate-Ca(2+)-N	1208:1224	alginate-Ca(2+)-N	1208:1224	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	7	55	theme	material	1198:1205	arg1	properties					1169:1178	The mechanical properties	1154:1178	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica)	1154:1244	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	10	56	theme	polycationic	1724:1735	arg1	poly					1737:1740	polycationic poly	1724:1740	polycationic poly(L-Lys)	1724:1747	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	10	56	theme	polycationic	1724:1735	arg1	L-Lys					1742:1746	L-Lys	1742:1746	L-Lys	1742:1746	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	11	57	theme	human	2005:2009	arg1	plasma					2011:2016	human plasma	2005:2016	human plasma	2005:2016	No significant effect of the polyP samples on the clotting of human plasma is measured.
26204529	6	58	theme	vessel	1138:1143	arg1	surface					1145:1151	the vessel surface	1134:1151	the vessel surface	1134:1151	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	6	59	theme	RGD	1034:1036	arg1	peptide					1038:1044	a His/Gly-tagged RGD peptide	1017:1044	a His/Gly-tagged RGD peptide (three RGD units)	1017:1062	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	6	59	theme	RGD	1034:1036	arg1	units					1057:1061	three RGD units	1047:1061	three RGD units	1047:1061	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	1	60	theme	tissue-engineered	175:191	arg1	bTEBV					208:212	bTEBV	208:212	bTEBV	208:212	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	1	60	theme	tissue-engineered	175:191	arg1	vessels					199:205	biomimetic tissue-engineered blood vessels	164:205	biomimetic tissue-engineered blood vessels (bTEBV)	164:213	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	6	61	theme	His/Gly-tagged	1019:1032	arg1	peptide					1038:1044	a His/Gly-tagged RGD peptide	1017:1044	a His/Gly-tagged RGD peptide (three RGD units)	1017:1062	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	6	61	theme	His/Gly-tagged	1019:1032	arg1	units					1057:1061	three RGD units	1047:1061	three RGD units	1047:1061	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	10	62	theme	poly	1750:1753	arg1	Incorporation					1707:1719	Incorporation	1707:1719	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide,	1707:1808	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	4	63	theme	2+	784:785	arg1	O-CMC-polyP					790:800	O-CMC-polyP	790:800	O-CMC-polyP	790:800	These materials can be hardened by exposure to Ca(2+) through formation of Ca(2+) bridges between the polyanions, alginate, N,O-CMC, and polyP (alginate-Ca(2+)-N,O-CMC-polyP).
26204529	4	63	theme	2+	784:785	arg1	-N					787:788	alginate-Ca(2+)-N	772:788	alginate-Ca(2+)-N	772:788	These materials can be hardened by exposure to Ca(2+) through formation of Ca(2+) bridges between the polyanions, alginate, N,O-CMC, and polyP (alginate-Ca(2+)-N,O-CMC-polyP).
26204529	6	64	theme	biomaterial	956:966	arg1	scaffold					934:941	this universal scaffold	919:941	this universal scaffold of the bTEBV biomaterial	919:966	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	8	65	theme	grafts	1391:1396	arg1	durable					1512:1518	durable	1512:1518	durable	1512:1518	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	8	65	theme	grafts	1391:1396	arg1	material					1355:1362	The material	1351:1362	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively)	1351:1493	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	8	66	theme	inner	1449:1453	arg1	mm					1466:1467	an inner diameter 4 mm and 0.8 mm	1446:1478	mm	1466:1467	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	1	67	theme	type	108:111	arg1	fabrication					85:95	the fabrication	81:95	the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition	81:241	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	7	68	theme	CaCl2	1335:1339	arg1	solution					1341:1348	CaCl2 solution	1335:1348	CaCl2 solution	1335:1348	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	1	69	theme	artificial	116:125	arg1	vessels					148:154	artificial small diameter blood vessels	116:154	artificial small diameter blood vessels	116:154	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	4	70	theme	alginate-Ca	772:782	arg1	O-CMC-polyP					790:800	O-CMC-polyP	790:800	O-CMC-polyP	790:800	These materials can be hardened by exposure to Ca(2+) through formation of Ca(2+) bridges between the polyanions, alginate, N,O-CMC, and polyP (alginate-Ca(2+)-N,O-CMC-polyP).
26204529	4	70	theme	alginate-Ca	772:782	arg1	-N					787:788	alginate-Ca(2+)-N	772:788	alginate-Ca(2+)-N	772:788	These materials can be hardened by exposure to Ca(2+) through formation of Ca(2+) bridges between the polyanions, alginate, N,O-CMC, and polyP (alginate-Ca(2+)-N,O-CMC-polyP).
26204529	10	71	theme	His/Gly-tagged	1782:1795	arg1	peptide					1801:1807	the His/Gly-tagged RGD peptide	1778:1807	the His/Gly-tagged RGD peptide	1778:1807	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	2	72	theme	charged	314:320	arg1	polymers					330:337	two negatively charged natural polymers	299:337	two negatively charged natural polymers	299:337	They are composed of a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC).
26204529	8	73	theme	mm	1623:1624	arg1	Hg					1626:1627	18.7 and 75.0 mm Hg	1609:1627	18.7 and 75.0 mm Hg	1609:1627	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	8	73	theme	mm	1623:1624	arg1	mbar					1603:1606	25 and 100 mbar	1592:1606	25 and 100 mbar (18.7 and 75.0 mm Hg)	1592:1628	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	1	74	theme	diameter	133:140	arg1	vessels					148:154	artificial small diameter blood vessels	116:154	artificial small diameter blood vessels	116:154	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	12	75	theme	promising	2106:2114	arg1	biomaterial					2116:2126	a promising biomaterial	2104:2126	a promising biomaterial for future prosthetic vascular grafts	2104:2164	We propose that the metabolically degradable polymeric scaffold bTEBV is a promising biomaterial for future prosthetic vascular grafts.
26204529	12	75	theme	promising	2106:2114	arg1	bTEBV					2095:2099	the metabolically degradable polymeric scaffold bTEBV	2047:2099	the metabolically degradable polymeric scaffold bTEBV	2047:2099	We propose that the metabolically degradable polymeric scaffold bTEBV is a promising biomaterial for future prosthetic vascular grafts.
26204529	7	76	theme	short	1308:1312	arg1	period					1325:1330	a short incubation period	1306:1330	a short incubation period in CaCl2 solution	1306:1348	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	9	77	theme	burst	1635:1639	arg1	mbar					1690:1693	850 mbar	1686:1693	850 mbar (145 mbar)	1686:1704	The burst pressure of the larger (smaller) vessels was 850 mbar (145 mbar).
26204529	9	77	theme	burst	1635:1639	arg1	pressure					1641:1648	The burst pressure	1631:1648	The burst pressure of the larger (smaller) vessels	1631:1680	The burst pressure of the larger (smaller) vessels was 850 mbar (145 mbar).
26204529	8	78	from	durable	1512:1518	arg1	experiments					1545:1555	4-week pulsatile flow experiments	1523:1555	4-week pulsatile flow experiments	1523:1555	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	1	79	theme	vessels	148:154	arg1	type					108:111	a novel type	100:111	a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV),	100:214	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
26204529	11	80	theme	significant	1946:1956	arg1	effect					1958:1963	No significant effect	1943:1963	No significant effect of the polyP samples on the clotting of human plasma	1943:2016	No significant effect of the polyP samples on the clotting of human plasma is measured.
26204529	7	81	theme	kPa	1291:1293	arg1	hardness					1257:1264	a hardness	1255:1264	a hardness (elastic modulus) of 475 kPa	1255:1293	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	7	81	theme	kPa	1291:1293	arg1	modulus					1275:1281	elastic modulus	1267:1281	elastic modulus	1267:1281	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	9	82	theme	larger	1657:1662	arg1	vessels					1674:1680	the larger (smaller) vessels	1653:1680	the larger (smaller) vessels	1653:1680	The burst pressure of the larger (smaller) vessels was 850 mbar (145 mbar).
26204529	6	83	theme	cells	1125:1129	arg1	adhesion					1101:1108	the adhesion	1097:1108	the adhesion of endothelial cells to the vessel surface	1097:1151	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	11	84	theme	polyP	1972:1976	arg1	samples					1978:1984	the polyP samples	1968:1984	the polyP samples	1968:1984	No significant effect of the polyP samples on the clotting of human plasma is measured.
26204529	8	85	dep	grafts	1391:1396	arg1	bTEBVs					1399:1404	bTEBVs	1399:1404	bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively	1399:1492	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	0	86	theme	Small	8:12	arg1	Grafts					32:37	Modular Small Diameter Vascular Grafts	0:37	Modular Small Diameter Vascular Grafts with Bioactive Functionalities	0:68	Modular Small Diameter Vascular Grafts with Bioactive Functionalities.
26204529	8	87	from	experiments	1545:1555	arg1	durable					1512:1518	durable	1512:1518	durable	1512:1518	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	8	87	from	experiments	1545:1555	arg1	material					1355:1362	The material	1351:1362	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively)	1351:1493	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	9	88	theme	vessels	1674:1680	arg1	mbar					1690:1693	850 mbar	1686:1693	850 mbar (145 mbar)	1686:1704	The burst pressure of the larger (smaller) vessels was 850 mbar (145 mbar).
26204529	9	88	theme	vessels	1674:1680	arg1	pressure					1641:1648	The burst pressure	1631:1648	The burst pressure of the larger (smaller) vessels	1631:1680	The burst pressure of the larger (smaller) vessels was 850 mbar (145 mbar).
26204529	2	89	theme	modified	355:362	arg1	N					374:374	N	374:374	N	374:374	They are composed of a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC).
26204529	2	89	theme	modified	355:362	arg1	chitosan					392:399	O-carboxymethyl chitosan	376:399	O-carboxymethyl chitosan (N,O-CMC)	376:409	They are composed of a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC).
26204529	2	89	theme	modified	355:362	arg1	chitosan					364:371	a modified chitosan	353:371	a modified chitosan	353:371	They are composed of a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC).
26204529	0	90	theme	Vascular	23:30	arg1	Grafts					32:37	Modular Small Diameter Vascular Grafts	0:37	Modular Small Diameter Vascular Grafts with Bioactive Functionalities	0:68	Modular Small Diameter Vascular Grafts with Bioactive Functionalities.
26204529	8	91	from	pressure	1575:1582	arg1	durable					1512:1518	durable	1512:1518	durable	1512:1518	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	8	91	from	pressure	1575:1582	arg1	material					1355:1362	The material	1351:1362	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively)	1351:1493	The material of the artificial vascular grafts (bTEBVs with an outer size 6 mm and 1.8 mm, and an inner diameter 4 mm and 0.8 mm, respectively) turned out to be durable in 4-week pulsatile flow experiments at an alternating pressure between 25 and 100 mbar (18.7 and 75.0 mm Hg).
26204529	3	92	theme	cell	584:587	arg1	signal					601:606	the cell recognition signal	580:606	the cell recognition signal	580:606	Into this biologically inert scaffold two biofunctionally active biopolymers are embedded, inorganic polyphosphate (polyP) and silica, as well as gelatin which exposes the cell recognition signal, Arg-Gly-Asp (RGD).
26204529	3	92	theme	cell	584:587	arg1	Arg-Gly-Asp					609:619	Arg-Gly-Asp	609:619	Arg-Gly-Asp (RGD)	609:625	Into this biologically inert scaffold two biofunctionally active biopolymers are embedded, inorganic polyphosphate (polyP) and silica, as well as gelatin which exposes the cell recognition signal, Arg-Gly-Asp (RGD).
26204529	4	93	theme	bridges	710:716	arg1	formation					690:698	formation	690:698	formation of Ca(2+) bridges between the polyanions, alginate, N,O-CMC, and polyP (alginate-Ca(2+)-N,O-CMC-polyP)	690:801	These materials can be hardened by exposure to Ca(2+) through formation of Ca(2+) bridges between the polyanions, alginate, N,O-CMC, and polyP (alginate-Ca(2+)-N,O-CMC-polyP).
26204529	6	94	theme	bTEBV	950:954	arg1	biomaterial					956:966	the bTEBV biomaterial	946:966	the bTEBV biomaterial	946:966	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	2	95	dep	chitosan	392:399	arg1	O-CMC					404:408	O-CMC	404:408	O-CMC	404:408	They are composed of a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC).
26204529	2	95	dep	chitosan	392:399	arg1	N					402:402	N	402:402	N	402:402	They are composed of a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC).
26204529	2	96	theme	natural	322:328	arg1	polymers					330:337	two negatively charged natural polymers	299:337	two negatively charged natural polymers	299:337	They are composed of a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC).
26204529	9	97	dep	larger	1657:1662	arg1	smaller					1665:1671	smaller	1665:1671	smaller	1665:1671	The burst pressure of the larger (smaller) vessels was 850 mbar (145 mbar).
26204529	7	98	from	period	1325:1330	arg1	solution					1341:1348	CaCl2 solution	1335:1348	CaCl2 solution	1335:1348	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	7	99	theme	elastic	1267:1273	arg1	hardness					1257:1264	a hardness	1255:1264	a hardness (elastic modulus) of 475 kPa	1255:1293	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	7	99	theme	elastic	1267:1273	arg1	modulus					1275:1281	elastic modulus	1267:1281	elastic modulus	1267:1281	The mechanical properties of the biopolymer material (alginate-Ca(2+)-N,O-CMC-polyP-silica) revealed a hardness (elastic modulus) of 475 kPa even after a short incubation period in CaCl2 solution.
26204529	10	100	theme	endothelial	1876:1886	arg1	cells					1888:1892	human, umbilical vein/vascular endothelial cells	1845:1892	cells	1888:1892	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	10	100	theme	endothelial	1876:1886	arg1	cells					1904:1908	EA.HY926 cells	1895:1908	EA.HY926 cells	1895:1908	Incorporation of polycationic poly(L-Lys), poly(D-Lys), and especially the His/Gly-tagged RGD peptide, markedly increased the adhesion of human, umbilical vein/vascular endothelial cells, EA.HY926 cells, to the surface of the hydrogel.
26204529	4	101	theme	Ca	703:704	arg1	bridges					710:716	Ca(2+) bridges	703:716	Ca(2+) bridges	703:716	These materials can be hardened by exposure to Ca(2+) through formation of Ca(2+) bridges between the polyanions, alginate, N,O-CMC, and polyP (alginate-Ca(2+)-N,O-CMC-polyP).
26204529	6	102	theme	universal	924:932	arg1	scaffold					934:941	this universal scaffold	919:941	this universal scaffold of the bTEBV biomaterial	919:966	In this universal scaffold of the bTEBV biomaterial, polycations such as poly(L-Lys), poly(D-Lys) or a His/Gly-tagged RGD peptide (three RGD units) were incorporated, which promote the adhesion of endothelial cells to the vessel surface.
26204529	1	103	theme	modular	223:229	arg1	composition					231:241	a modular composition	221:241	a modular composition	221:241	We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition.
28089930	5	0	theme	Raman	687:691	arg1	spectra					693:699	Raman spectra	687:699	Raman spectra	687:699	These scaffolds (GO/CS/Gn) were characterized by the SEM, Raman spectra, FT-IR, EDS, swelling, biodegradation, protein adsorption and biomineralization studies.
28089930	8	1	theme	bone	1090:1093	arg1	defect					1095:1100	the rat tibial bone defect	1075:1100	the rat tibial bone defect	1075:1100	The scaffolds also accelerated bridging of the rat tibial bone defect with increased collagen deposition in vivo.
28089930	0	2	theme	tissue	67:72	arg1	regeneration					74:85	bone tissue regeneration	62:85	bone tissue regeneration	62:85	Scaffolds containing chitosan, gelatin and graphene oxide for bone tissue regeneration in vitro and in vivo.
28089930	6	3	theme	CS/Gn	817:821	arg1	scaffolds					823:831	the CS/Gn scaffolds	813:831	the CS/Gn scaffolds	813:831	The inclusion of GO in the CS/Gn scaffolds showed better physico-chemical properties.
28089930	6	4	from	inclusion	794:802	arg1	scaffolds					823:831	the CS/Gn scaffolds	813:831	the CS/Gn scaffolds	813:831	The inclusion of GO in the CS/Gn scaffolds showed better physico-chemical properties.
28089930	9	5	theme	bone	1254:1257	arg1	regeneration					1266:1277	bone tissue regeneration	1254:1277	bone tissue regeneration	1254:1277	Hence, these results strongly suggested the potential nature of GO/CS/Gn scaffolds for their application in bone tissue regeneration.
28089930	6	6	theme	physico-chemical	847:862	arg1	properties					864:873	better physico-chemical properties	840:873	better physico-chemical properties	840:873	The inclusion of GO in the CS/Gn scaffolds showed better physico-chemical properties.
28089930	2	7	theme	multiple	278:285	arg1	polymers					287:294	multiple polymers	278:294	multiple polymers	278:294	Improving the properties of three-dimensional scaffolds with multiple polymers and others is of growing interest in recent decades.
28089930	8	8	theme	collagen	1117:1124	arg1	deposition					1126:1135	increased collagen deposition	1107:1135	increased collagen deposition in vivo	1107:1143	The scaffolds also accelerated bridging of the rat tibial bone defect with increased collagen deposition in vivo.
28089930	6	9	theme	GO	807:808	arg1	inclusion					794:802	The inclusion	790:802	The inclusion of GO in the CS/Gn scaffolds	790:831	The inclusion of GO in the CS/Gn scaffolds showed better physico-chemical properties.
28089930	3	10	contain	has	385:387	arg2	limitations					389:399	limitations	389:399	limitations for its use in bone regeneration	389:432	Chitosan (CS), a natural biopolymer has limitations for its use in bone regeneration, and its properties can be enhanced with other materials.
28089930	3	10	contain	has	385:387	arg1	biopolymer					374:383	a natural biopolymer	364:383	a natural biopolymer	364:383	Chitosan (CS), a natural biopolymer has limitations for its use in bone regeneration, and its properties can be enhanced with other materials.
28089930	3	10	contain	has	385:387	arg1	CS					359:360	CS	359:360	CS	359:360	Chitosan (CS), a natural biopolymer has limitations for its use in bone regeneration, and its properties can be enhanced with other materials.
28089930	3	10	contain	has	385:387	arg1	Chitosan					349:356	Chitosan	349:356	Chitosan (CS)	349:361	Chitosan (CS), a natural biopolymer has limitations for its use in bone regeneration, and its properties can be enhanced with other materials.
28089930	1	11	theme	cell	156:159	arg1	constructs					182:191	cell free and cell loaded constructs	156:191	cell free and cell loaded constructs to bridge bone defects	156:214	Critical-sized bone defects are augmented with cell free and cell loaded constructs to bridge bone defects.
28089930	8	12	theme	increased	1107:1115	arg1	deposition					1126:1135	increased collagen deposition	1107:1135	increased collagen deposition in vivo	1107:1143	The scaffolds also accelerated bridging of the rat tibial bone defect with increased collagen deposition in vivo.
28089930	2	13	theme	scaffolds	263:271	arg1	properties					231:240	the properties	227:240	the properties of three-dimensional scaffolds with multiple polymers and others	227:305	Improving the properties of three-dimensional scaffolds with multiple polymers and others is of growing interest in recent decades.
28089930	1	14	theme	free	161:164	arg1	constructs					182:191	cell free and cell loaded constructs	156:191	cell free and cell loaded constructs to bridge bone defects	156:214	Critical-sized bone defects are augmented with cell free and cell loaded constructs to bridge bone defects.
28089930	8	15	theme	tibial	1083:1088	arg1	defect					1095:1100	the rat tibial bone defect	1075:1100	the rat tibial bone defect	1075:1100	The scaffolds also accelerated bridging of the rat tibial bone defect with increased collagen deposition in vivo.
28089930	8	16	theme	rat	1079:1081	arg1	defect					1095:1100	the rat tibial bone defect	1075:1100	the rat tibial bone defect	1075:1100	The scaffolds also accelerated bridging of the rat tibial bone defect with increased collagen deposition in vivo.
28089930	9	17	theme	tissue	1259:1264	arg1	regeneration					1266:1277	bone tissue regeneration	1254:1277	bone tissue regeneration	1254:1277	Hence, these results strongly suggested the potential nature of GO/CS/Gn scaffolds for their application in bone tissue regeneration.
28089930	7	18	theme	mesenchymal	991:1001	arg1	cells					1008:1012	mouse mesenchymal stem cells	985:1012	mouse mesenchymal stem cells	985:1012	The GO/CS/Gn scaffolds were cyto-friendly to rat osteoprogenitor cells, and they promoted differentiation of mouse mesenchymal stem cells into osteoblasts.
28089930	7	19	theme	cells	1008:1012	arg1	differentiation					966:980	differentiation	966:980	differentiation of mouse mesenchymal stem cells into osteoblasts	966:1029	The GO/CS/Gn scaffolds were cyto-friendly to rat osteoprogenitor cells, and they promoted differentiation of mouse mesenchymal stem cells into osteoblasts.
28089930	7	20	theme	GO/CS/Gn	880:887	arg1	scaffolds					889:897	The GO/CS/Gn scaffolds	876:897	The GO/CS/Gn scaffolds	876:897	The GO/CS/Gn scaffolds were cyto-friendly to rat osteoprogenitor cells, and they promoted differentiation of mouse mesenchymal stem cells into osteoblasts.
28089930	7	20	theme	GO/CS/Gn	880:887	arg1	cells					941:945	rat osteoprogenitor cells	921:945	rat osteoprogenitor cells	921:945	The GO/CS/Gn scaffolds were cyto-friendly to rat osteoprogenitor cells, and they promoted differentiation of mouse mesenchymal stem cells into osteoblasts.
28089930	1	21	theme	loaded	175:180	arg1	constructs					182:191	cell free and cell loaded constructs	156:191	cell free and cell loaded constructs to bridge bone defects	156:214	Critical-sized bone defects are augmented with cell free and cell loaded constructs to bridge bone defects.
28089930	7	22	theme	osteoprogenitor	925:939	arg1	scaffolds					889:897	The GO/CS/Gn scaffolds	876:897	The GO/CS/Gn scaffolds	876:897	The GO/CS/Gn scaffolds were cyto-friendly to rat osteoprogenitor cells, and they promoted differentiation of mouse mesenchymal stem cells into osteoblasts.
28089930	7	22	theme	osteoprogenitor	925:939	arg1	cells					941:945	rat osteoprogenitor cells	921:945	rat osteoprogenitor cells	921:945	The GO/CS/Gn scaffolds were cyto-friendly to rat osteoprogenitor cells, and they promoted differentiation of mouse mesenchymal stem cells into osteoblasts.
28089930	4	23	theme	composite	518:526	arg1	scaffolds					528:536	the composite scaffolds	514:536	the composite scaffolds containing CS, gelatin (Gn) and graphene oxide (GO)	514:588	In the present study, the composite scaffolds containing CS, gelatin (Gn) and graphene oxide (GO) were fabricated through freeze-drying.
28089930	5	24	theme	protein	740:746	arg1	adsorption					748:757	protein adsorption	740:757	protein adsorption	740:757	These scaffolds (GO/CS/Gn) were characterized by the SEM, Raman spectra, FT-IR, EDS, swelling, biodegradation, protein adsorption and biomineralization studies.
28089930	9	25	from	application	1239:1249	arg1	regeneration					1266:1277	bone tissue regeneration	1254:1277	bone tissue regeneration	1254:1277	Hence, these results strongly suggested the potential nature of GO/CS/Gn scaffolds for their application in bone tissue regeneration.
28089930	5	26	dep	SEM	682:684	arg1	the					678:680	the	678:680	the	678:680	These scaffolds (GO/CS/Gn) were characterized by the SEM, Raman spectra, FT-IR, EDS, swelling, biodegradation, protein adsorption and biomineralization studies.
28089930	3	27	theme	bone	416:419	arg1	regeneration					421:432	bone regeneration	416:432	bone regeneration	416:432	Chitosan (CS), a natural biopolymer has limitations for its use in bone regeneration, and its properties can be enhanced with other materials.
28089930	5	28	theme	biomineralization	763:779	arg1	studies					781:787	biomineralization studies	763:787	biomineralization studies	763:787	These scaffolds (GO/CS/Gn) were characterized by the SEM, Raman spectra, FT-IR, EDS, swelling, biodegradation, protein adsorption and biomineralization studies.
28089930	7	29	theme	stem	1003:1006	arg1	cells					1008:1012	mouse mesenchymal stem cells	985:1012	mouse mesenchymal stem cells	985:1012	The GO/CS/Gn scaffolds were cyto-friendly to rat osteoprogenitor cells, and they promoted differentiation of mouse mesenchymal stem cells into osteoblasts.
28089930	4	30	dep	oxide	579:583	arg1	GO					586:587	GO	586:587	GO	586:587	In the present study, the composite scaffolds containing CS, gelatin (Gn) and graphene oxide (GO) were fabricated through freeze-drying.
28089930	1	31	theme	bridge	196:201	arg1	defects					208:214	bridge bone defects	196:214	bridge bone defects	196:214	Critical-sized bone defects are augmented with cell free and cell loaded constructs to bridge bone defects.
28089930	3	32	theme	natural	366:372	arg1	biopolymer					374:383	a natural biopolymer	364:383	a natural biopolymer	364:383	Chitosan (CS), a natural biopolymer has limitations for its use in bone regeneration, and its properties can be enhanced with other materials.
28089930	3	32	theme	natural	366:372	arg1	Chitosan					349:356	Chitosan	349:356	Chitosan (CS)	349:361	Chitosan (CS), a natural biopolymer has limitations for its use in bone regeneration, and its properties can be enhanced with other materials.
28089930	9	33	theme	potential	1190:1198	arg1	nature					1200:1205	the potential nature	1186:1205	the potential nature of GO/CS/Gn scaffolds for their application in bone tissue regeneration	1186:1277	Hence, these results strongly suggested the potential nature of GO/CS/Gn scaffolds for their application in bone tissue regeneration.
28089930	2	34	theme	recent	333:338	arg1	decades					340:346	recent decades	333:346	recent decades	333:346	Improving the properties of three-dimensional scaffolds with multiple polymers and others is of growing interest in recent decades.
28089930	0	35	theme	graphene	43:50	arg1	oxide					52:56	graphene oxide	43:56	graphene oxide	43:56	Scaffolds containing chitosan, gelatin and graphene oxide for bone tissue regeneration in vitro and in vivo.
28089930	4	36	theme	present	499:505	arg1	study					507:511	the present study	495:511	the present study	495:511	In the present study, the composite scaffolds containing CS, gelatin (Gn) and graphene oxide (GO) were fabricated through freeze-drying.
28089930	7	37	theme	mouse	985:989	arg1	cells					1008:1012	mouse mesenchymal stem cells	985:1012	mouse mesenchymal stem cells	985:1012	The GO/CS/Gn scaffolds were cyto-friendly to rat osteoprogenitor cells, and they promoted differentiation of mouse mesenchymal stem cells into osteoblasts.
28089930	4	38	contain	containing	538:547	arg2	Gn					562:563	Gn	562:563	Gn	562:563	In the present study, the composite scaffolds containing CS, gelatin (Gn) and graphene oxide (GO) were fabricated through freeze-drying.
28089930	4	38	contain	containing	538:547	arg1	scaffolds					528:536	the composite scaffolds	514:536	the composite scaffolds containing CS, gelatin (Gn) and graphene oxide (GO)	514:588	In the present study, the composite scaffolds containing CS, gelatin (Gn) and graphene oxide (GO) were fabricated through freeze-drying.
28089930	4	38	contain	containing	538:547	arg2	CS					549:550	CS	549:550	CS	549:550	In the present study, the composite scaffolds containing CS, gelatin (Gn) and graphene oxide (GO) were fabricated through freeze-drying.
28089930	4	38	contain	containing	538:547	arg2	gelatin					553:559	gelatin	553:559	gelatin (Gn)	553:564	In the present study, the composite scaffolds containing CS, gelatin (Gn) and graphene oxide (GO) were fabricated through freeze-drying.
28089930	4	38	contain	containing	538:547	arg2	oxide					579:583	graphene oxide	570:583	graphene oxide (GO)	570:588	In the present study, the composite scaffolds containing CS, gelatin (Gn) and graphene oxide (GO) were fabricated through freeze-drying.
28089930	6	39	theme	better	840:845	arg1	properties					864:873	better physico-chemical properties	840:873	better physico-chemical properties	840:873	The inclusion of GO in the CS/Gn scaffolds showed better physico-chemical properties.
28089930	2	40	theme	three-dimensional	245:261	arg1	scaffolds					263:271	three-dimensional scaffolds	245:271	three-dimensional scaffolds with multiple polymers and others	245:305	Improving the properties of three-dimensional scaffolds with multiple polymers and others is of growing interest in recent decades.
28089930	1	41	theme	Critical-sized	109:122	arg1	defects					129:135	Critical-sized bone defects	109:135	Critical-sized bone defects	109:135	Critical-sized bone defects are augmented with cell free and cell loaded constructs to bridge bone defects.
28089930	1	42	theme	bone	203:206	arg1	defects					208:214	bridge bone defects	196:214	bridge bone defects	196:214	Critical-sized bone defects are augmented with cell free and cell loaded constructs to bridge bone defects.
28089930	9	43	theme	GO/CS/Gn	1210:1217	arg1	scaffolds					1219:1227	GO/CS/Gn scaffolds	1210:1227	GO/CS/Gn scaffolds	1210:1227	Hence, these results strongly suggested the potential nature of GO/CS/Gn scaffolds for their application in bone tissue regeneration.
28089930	7	44	theme	rat	921:923	arg1	scaffolds					889:897	The GO/CS/Gn scaffolds	876:897	The GO/CS/Gn scaffolds	876:897	The GO/CS/Gn scaffolds were cyto-friendly to rat osteoprogenitor cells, and they promoted differentiation of mouse mesenchymal stem cells into osteoblasts.
28089930	7	44	theme	rat	921:923	arg1	cells					941:945	rat osteoprogenitor cells	921:945	rat osteoprogenitor cells	921:945	The GO/CS/Gn scaffolds were cyto-friendly to rat osteoprogenitor cells, and they promoted differentiation of mouse mesenchymal stem cells into osteoblasts.
28089930	1	45	theme	bone	124:127	arg1	defects					129:135	Critical-sized bone defects	109:135	Critical-sized bone defects	109:135	Critical-sized bone defects are augmented with cell free and cell loaded constructs to bridge bone defects.
28089930	2	46	with	scaffolds	263:271	arg1	others					300:305	others	300:305	others	300:305	Improving the properties of three-dimensional scaffolds with multiple polymers and others is of growing interest in recent decades.
28089930	2	46	with	scaffolds	263:271	arg1	polymers					287:294	multiple polymers	278:294	multiple polymers	278:294	Improving the properties of three-dimensional scaffolds with multiple polymers and others is of growing interest in recent decades.
28089930	0	47	theme	bone	62:65	arg1	regeneration					74:85	bone tissue regeneration	62:85	bone tissue regeneration	62:85	Scaffolds containing chitosan, gelatin and graphene oxide for bone tissue regeneration in vitro and in vivo.
28089930	9	48	theme	scaffolds	1219:1227	arg1	nature					1200:1205	the potential nature	1186:1205	the potential nature of GO/CS/Gn scaffolds for their application in bone tissue regeneration	1186:1277	Hence, these results strongly suggested the potential nature of GO/CS/Gn scaffolds for their application in bone tissue regeneration.
28089930	1	49	dep	loaded	175:180	arg1	cell					170:173	cell	170:173	cell	170:173	Critical-sized bone defects are augmented with cell free and cell loaded constructs to bridge bone defects.
28089930	4	50	theme	graphene	570:577	arg1	oxide					579:583	graphene oxide	570:583	graphene oxide (GO)	570:588	In the present study, the composite scaffolds containing CS, gelatin (Gn) and graphene oxide (GO) were fabricated through freeze-drying.
28089930	3	51	theme	other	475:479	arg1	materials					481:489	other materials	475:489	other materials	475:489	Chitosan (CS), a natural biopolymer has limitations for its use in bone regeneration, and its properties can be enhanced with other materials.
28089930	3	52	from	use	409:411	arg1	regeneration					421:432	bone regeneration	416:432	bone regeneration	416:432	Chitosan (CS), a natural biopolymer has limitations for its use in bone regeneration, and its properties can be enhanced with other materials.
26583778	2	0	theme	fermentable	783:793	arg1	inulin					806:811	inulin	806:811	inulin	806:811	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	2	0	theme	fermentable	783:793	arg1	substrate					795:803	a highly fermentable substrate	774:803	a highly fermentable substrate (inulin)	774:812	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	5	1	theme	grain	1332:1336	arg1	intakes					1277:1283	intakes	1277:1283	intakes	1277:1283	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	2	2	from	fermentation	855:866	arg1	results					838:844	results	838:844	results from the fermentation of inulin	838:876	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	5	3	theme	dry	1339:1341	arg1	beans					1343:1347	dry beans	1339:1347	dry beans	1339:1347	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	1	4	theme	microbiota	224:233	arg1	activity					203:210	metabolic activity	193:210	metabolic activity	193:210	Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease.
26583778	1	4	theme	microbiota	224:233	arg1	functionality					175:187	the functionality	171:187	the functionality	171:187	Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease.
26583778	5	5	theme	foods	1308:1312	arg1	intakes					1277:1283	intakes	1277:1283	intakes	1277:1283	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	7	6	theme	butyrate	2014:2021	arg1	metabolism					2002:2011	beneficial metabolism	1991:2011	beneficial metabolism (butyrate production)	1991:2033	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	7	6	theme	butyrate	2014:2021	arg1	production					2023:2032	butyrate production	2014:2032	butyrate production	2014:2032	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	2	7	theme	branched	518:525	arg1	concentrations					553:566	fecal short and branched chain fatty acid (S/BCFA) concentrations	502:566	fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr	502:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	0	8	theme	inulin	100:105	arg1	properties					86:95	in vitro fecal fermentation properties	58:95	in vitro fecal fermentation properties of inulin	58:105	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.
26583778	2	9	theme	short	508:512	arg1	concentrations					553:566	fecal short and branched chain fatty acid (S/BCFA) concentrations	502:566	fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr	502:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	6	10	theme	dairy	1562:1566	arg1	meats					1582:1586	dairy and processed meats that provided cholesterol and little fiber,	1562:1630	meats	1582:1586	In contrast, intake of dairy and processed meats that provided cholesterol and little fiber, were associated with ammonia and BCFA production.
26583778	2	11	theme	ammonia	717:723	arg1	production					725:734	ammonia production	717:734	ammonia production	717:734	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	1	12	theme	beneficial	350:359	arg1	metabolites					361:371	beneficial metabolites	350:371	beneficial metabolites that protect against disease	350:400	Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease.
26583778	2	13	theme	fermentable	934:944	arg1	substrate					946:954	a poorly fermentable substrate	925:954	a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr	925:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	2	14	theme	branched	666:673	arg1	production					701:710	short and branched chain fatty acid (S/BCFA) production	656:710	short and branched chain fatty acid (S/BCFA) production	656:710	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	7	15	theme	microbiota	1966:1975	arg1	propensity					1944:1953	the propensity	1940:1953	the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production)	1940:2077	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	5	16	theme	SCFA	1495:1498	arg1	production					1500:1509	total SCFA production	1489:1509	total SCFA production during inulin fermentation	1489:1536	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	2	17	theme	fatty	533:537	arg1	concentrations					553:566	fecal short and branched chain fatty acid (S/BCFA) concentrations	502:566	fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr	502:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	4	18	theme	dietary	1085:1091	arg1	records					1093:1099	one-year dietary records	1076:1099	one-year dietary records	1076:1099	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	2	19	theme	short	656:660	arg1	production					701:710	short and branched chain fatty acid (S/BCFA) production	656:710	short and branched chain fatty acid (S/BCFA) production	656:710	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	8	20	theme	new	2125:2127	arg1	strategies					2137:2146	new dietary strategies	2125:2146	new dietary strategies	2125:2146	These results may help in the development of new dietary strategies to improve gut microbiota functionality to promote human health.
26583778	7	21	contain	have	1912:1915	arg1	diet					1903:1906	long-term diet	1893:1906	long-term diet	1893:1906	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	7	21	contain	have	1912:1915	arg2	effect					1930:1935	a pronounced effect	1917:1935	a pronounced effect	1917:1935	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	5	22	theme	Few	1189:1191	arg1	variables					1208:1216	Few dietary intake variables	1189:1216	Few dietary intake variables	1189:1216	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	0	23	theme	donor	49:53	arg1	pattern					32:38	long-term dietary pattern	14:38	long-term dietary pattern of fecal donor	14:53	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.
26583778	6	24	theme	little	1618:1623	arg1	fiber					1625:1629	little fiber	1618:1629	little fiber	1618:1629	In contrast, intake of dairy and processed meats that provided cholesterol and little fiber, were associated with ammonia and BCFA production.
26583778	2	25	theme	fatty	681:685	arg1	production					701:710	short and branched chain fatty acid (S/BCFA) production	656:710	short and branched chain fatty acid (S/BCFA) production	656:710	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	2	26	dep	substrate	946:954	arg1	wheat					963:967	whole wheat	957:967	whole wheat; Yang and Rose, Nutr	957:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	0	27	theme	in	58:59	arg1	properties					86:95	in vitro fecal fermentation properties	58:95	in vitro fecal fermentation properties of inulin	58:105	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.
26583778	1	28	theme	gut	140:142	arg1	microbiota					144:153	the gut microbiota	136:153	the gut microbiota	136:153	Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease.
26583778	5	29	theme	dietary	1387:1393	arg1	fiber					1395:1399	dietary fiber	1387:1399	dietary fiber	1387:1399	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	2	30	theme	intake	615:620	arg1	variables					622:630	dietary intake variables	607:630	dietary intake variables	607:630	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	0	31	theme	fecal	67:71	arg1	properties					86:95	in vitro fecal fermentation properties	58:95	in vitro fecal fermentation properties of inulin	58:105	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.
26583778	2	32	theme	intake	481:486	arg1	variables					488:496	dietary intake variables	473:496	dietary intake variables	473:496	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	4	33	theme	fecal	1126:1130	arg1	system					1145:1150	an in vitro fecal fermentation system	1114:1150	an in vitro fecal fermentation system with long-chain inulin	1114:1173	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	0	34	theme	pattern	32:38	arg1	impact					4:9	The impact	0:9	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.	0:106	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.
26583778	6	35	theme	processed	1572:1580	arg1	meats					1582:1586	dairy and processed meats that provided cholesterol and little fiber,	1562:1630	meats	1582:1586	In contrast, intake of dairy and processed meats that provided cholesterol and little fiber, were associated with ammonia and BCFA production.
26583778	5	36	theme	certain	1354:1360	arg1	vegetables					1362:1371	certain vegetables	1354:1371	certain vegetables	1354:1371	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	5	37	theme	S/BCFA	1245:1250	arg1	concentrations					1252:1265	fecal S/BCFA concentrations	1239:1265	fecal S/BCFA concentrations	1239:1265	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	0	38	dep	in	58:59	arg1	vitro					61:65	vitro	61:65	vitro	61:65	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.
26583778	6	39	theme	BCFA	1665:1668	arg1	production					1670:1679	BCFA production	1665:1679	BCFA production	1665:1679	In contrast, intake of dairy and processed meats that provided cholesterol and little fiber, were associated with ammonia and BCFA production.
26583778	5	40	theme	intake	1201:1206	arg1	variables					1208:1216	Few dietary intake variables	1189:1216	Few dietary intake variables	1189:1216	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	1	41	theme	equal	241:245	arg1	importance					247:256	equal importance	241:256	equal importance	241:256	Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease.
26583778	8	42	theme	strategies	2137:2146	arg1	development					2110:2120	the development	2106:2120	the development of new dietary strategies	2106:2146	These results may help in the development of new dietary strategies to improve gut microbiota functionality to promote human health.
26583778	0	43	theme	dietary	24:30	arg1	pattern					32:38	long-term dietary pattern	14:38	long-term dietary pattern of fecal donor	14:53	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.
26583778	7	44	theme	whole	1719:1723	arg1	fermentations					1731:1743	inulin and whole wheat fermentations	1708:1743	inulin and whole wheat fermentations	1708:1743	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	2	45	theme	study	424:428	arg1	purposes					407:414	The purposes	403:414	The purposes of this study	403:428	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	1	46	theme	gut	263:265	arg1	microbiota					267:276	the gut microbiota	259:276	the gut microbiota	259:276	Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease.
26583778	8	47	theme	microbiota	2163:2172	arg1	functionality					2174:2186	gut microbiota functionality	2159:2186	gut microbiota functionality	2159:2186	These results may help in the development of new dietary strategies to improve gut microbiota functionality to promote human health.
26583778	2	48	dep	in	743:744	arg1	vitro					746:750	vitro	746:750	vitro	746:750	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	5	49	theme	plant-based	1296:1306	arg1	foods					1308:1312	several plant-based foods	1288:1312	several plant-based foods	1288:1312	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	2	50	theme	fecal	752:756	arg1	fermentation					758:769	in vitro fecal fermentation	743:769	in vitro fecal fermentation of a highly fermentable substrate (inulin)	743:812	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	4	51	theme	long-chain	1157:1166	arg1	inulin					1168:1173	long-chain inulin	1157:1173	long-chain inulin	1157:1173	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	7	52	theme	detrimental	2038:2048	arg1	metabolism					2050:2059	detrimental metabolism	2038:2059	detrimental metabolism (BCFA production)	2038:2077	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	7	52	theme	detrimental	2038:2048	arg1	production					2067:2076	BCFA production	2062:2076	BCFA production	2062:2076	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	8	53	theme	human	2199:2203	arg1	health					2205:2210	human health	2199:2210	human health	2199:2210	These results may help in the development of new dietary strategies to improve gut microbiota functionality to promote human health.
26583778	2	54	theme	substrate	795:803	arg1	fermentation					758:769	in vitro fecal fermentation	743:769	in vitro fecal fermentation of a highly fermentable substrate (inulin)	743:812	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	4	55	dep	in	1117:1118	arg1	vitro					1120:1124	vitro	1120:1124	vitro	1120:1124	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	1	56	theme	gut	220:222	arg1	microbiota					224:233	the gut microbiota	216:233	the gut microbiota	216:233	Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease.
26583778	2	57	theme	S/BCFA	545:550	arg1	concentrations					553:566	fecal short and branched chain fatty acid (S/BCFA) concentrations	502:566	fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr	502:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	4	58	theme	Stool	1017:1021	arg1	substrate					1178:1186	substrate	1178:1186	substrate	1178:1186	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	4	58	theme	Stool	1017:1021	arg1	samples					1023:1029	Stool samples	1017:1029	Stool samples from eighteen individuals that had completed one-year dietary records	1017:1099	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	5	59	theme	beans	1343:1347	arg1	intakes					1277:1283	intakes	1277:1283	intakes	1277:1283	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	1	60	theme	microbiota	144:153	arg1	composition					121:131	the composition	117:131	the composition of the gut microbiota	117:153	Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease.
26583778	7	61	theme	long-term	1893:1901	arg1	diet					1903:1906	long-term diet	1893:1906	long-term diet	1893:1906	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	2	62	theme	in	743:744	arg1	fermentation					758:769	in vitro fecal fermentation	743:769	in vitro fecal fermentation of a highly fermentable substrate (inulin)	743:812	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	5	63	theme	inulin	1518:1523	arg1	fermentation					1525:1536	inulin fermentation	1518:1536	inulin fermentation	1518:1536	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	5	64	theme	whole	1326:1330	arg1	grain					1332:1336	whole grain	1326:1336	whole grain	1326:1336	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	7	65	theme	inulin	1708:1713	arg1	fermentations					1731:1743	inulin and whole wheat fermentations	1708:1743	inulin and whole wheat fermentations	1708:1743	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	2	66	theme	whole	957:961	arg1	wheat					963:967	whole wheat	957:967	whole wheat; Yang and Rose, Nutr	957:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	4	67	used	used	1106:1109	arg2	substrate					1178:1186	substrate	1178:1186	substrate	1178:1186	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	4	67	used	used	1106:1109	arg2	samples					1023:1029	Stool samples	1017:1029	Stool samples from eighteen individuals that had completed one-year dietary records	1017:1099	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	7	68	theme	carbohydrate	1852:1863	arg1	type					1844:1847	the type	1840:1847	the type of carbohydrate provided to the microbiota	1840:1890	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	2	69	theme	fecal	502:506	arg1	concentrations					553:566	fecal short and branched chain fatty acid (S/BCFA) concentrations	502:566	fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr	502:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	2	70	theme	chain	675:679	arg1	production					701:710	short and branched chain fatty acid (S/BCFA) production	656:710	short and branched chain fatty acid (S/BCFA) production	656:710	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	4	71	with	system	1145:1150	arg1	inulin					1168:1173	long-chain inulin	1157:1173	long-chain inulin	1157:1173	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	5	72	theme	total	1489:1493	arg1	production					1500:1509	total SCFA production	1489:1509	total SCFA production during inulin fermentation	1489:1536	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	2	73	dep	concentrations	553:566	arg1	determine					572:580	determine	572:580	to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin)	569:812	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	2	73	dep	concentrations	553:566	arg1	compare					830:836	compare	830:836	to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr	827:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	2	74	theme	acid	539:542	arg1	concentrations					553:566	fecal short and branched chain fatty acid (S/BCFA) concentrations	502:566	fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr	502:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	4	75	theme	in	1117:1118	arg1	system					1145:1150	an in vitro fecal fermentation system	1114:1150	an in vitro fecal fermentation system with long-chain inulin	1114:1173	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	2	76	theme	chain	527:531	arg1	concentrations					553:566	fecal short and branched chain fatty acid (S/BCFA) concentrations	502:566	fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr	502:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	2	77	theme	S/BCFA	693:698	arg1	production					701:710	short and branched chain fatty acid (S/BCFA) production	656:710	short and branched chain fatty acid (S/BCFA) production	656:710	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	7	78	theme	gut	1962:1964	arg1	microbiota					1966:1975	the gut microbiota	1958:1975	the gut microbiota	1958:1975	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	2	79	dep	wheat	963:967	arg1	Nutr					985:988	Nutr	985:988	Nutr	985:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	2	79	dep	wheat	963:967	arg1	Rose					979:982	Rose	979:982	Rose	979:982	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	2	79	dep	wheat	963:967	arg1	Yang					970:973	Yang	970:973	Yang	970:973	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	2	80	theme	previous	903:910	arg1	report					912:917	a previous report	901:917	a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr	901:988	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	2	81	theme	acid	687:690	arg1	production					701:710	short and branched chain fatty acid (S/BCFA) production	656:710	short and branched chain fatty acid (S/BCFA) production	656:710	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	0	82	from	impact	4:9	arg1	properties					86:95	in vitro fecal fermentation properties	58:95	in vitro fecal fermentation properties of inulin	58:105	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.
26583778	0	83	theme	fermentation	73:84	arg1	properties					86:95	in vitro fecal fermentation properties	58:95	in vitro fecal fermentation properties of inulin	58:105	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.
26583778	1	84	theme	harmful	297:303	arg1	metabolites					305:315	harmful metabolites	297:315	harmful metabolites associated with human disease	297:345	Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease.
26583778	2	85	theme	dietary	607:613	arg1	variables					622:630	dietary intake variables	607:630	dietary intake variables	607:630	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	4	86	theme	fermentation	1132:1143	arg1	system					1145:1150	an in vitro fecal fermentation system	1114:1150	an in vitro fecal fermentation system with long-chain inulin	1114:1173	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	2	87	theme	dietary	473:479	arg1	variables					488:496	dietary intake variables	473:496	dietary intake variables	473:496	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	6	88	theme	meats	1582:1586	arg1	intake					1552:1557	intake	1552:1557	intake of dairy and processed meats that provided cholesterol and little fiber,	1552:1630	In contrast, intake of dairy and processed meats that provided cholesterol and little fiber, were associated with ammonia and BCFA production.
26583778	5	89	theme	fecal	1239:1243	arg1	concentrations					1252:1265	fecal S/BCFA concentrations	1239:1265	fecal S/BCFA concentrations	1239:1265	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	1	90	theme	human	333:337	arg1	disease					339:345	human disease	333:345	human disease	333:345	Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease.
26583778	5	91	theme	vegetables	1362:1371	arg1	intakes					1277:1283	intakes	1277:1283	intakes	1277:1283	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	2	92	theme	inulin	636:641	arg1	degradation					643:653	inulin degradation	636:653	inulin degradation	636:653	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	5	93	theme	dietary	1193:1199	arg1	variables					1208:1216	Few dietary intake variables	1189:1216	Few dietary intake variables	1189:1216	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	0	94	theme	long-term	14:22	arg1	pattern					32:38	long-term dietary pattern	14:38	long-term dietary pattern of fecal donor	14:53	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.
26583778	5	95	theme	B	1421:1421	arg1	vitamins					1423:1430	B vitamins	1421:1430	B vitamins	1421:1430	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	3	96	dep	Res.	991:994	arg1	749-759					1007:1013	749-759	1007:1013	749-759	1007:1013	Res., 2014, 34, 749-759).
26583778	8	97	theme	dietary	2129:2135	arg1	strategies					2137:2146	new dietary strategies	2125:2146	new dietary strategies	2125:2146	These results may help in the development of new dietary strategies to improve gut microbiota functionality to promote human health.
26583778	0	98	theme	fecal	43:47	arg1	donor					49:53	fecal donor	43:53	fecal donor	43:53	The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin.
26583778	7	99	theme	significant	1746:1756	arg1	correlations					1758:1769	significant correlations	1746:1769	significant correlations	1746:1769	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	7	100	theme	wheat	1725:1729	arg1	fermentations					1731:1743	inulin and whole wheat fermentations	1708:1743	inulin and whole wheat fermentations	1708:1743	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	2	101	theme	inulin	871:876	arg1	fermentation					855:866	the fermentation	851:866	the fermentation of inulin	851:876	The purposes of this study were to determine the associations between dietary intake variables and fecal short and branched chain fatty acid (S/BCFA) concentrations; to determine the associations between dietary intake variables and inulin degradation, short and branched chain fatty acid (S/BCFA) production, and ammonia production during in vitro fecal fermentation of a highly fermentable substrate (inulin); and finally to compare results from the fermentation of inulin with those obtained in a previous report using a poorly fermentable substrate (whole wheat; Yang and Rose, Nutr.
26583778	5	102	theme	plant	1402:1406	arg1	protein					1408:1414	plant protein	1402:1414	plant protein	1402:1414	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	5	103	theme	several	1288:1294	arg1	foods					1308:1312	several plant-based foods	1288:1312	several plant-based foods	1288:1312	Few dietary intake variables were correlated with fecal S/BCFA concentrations; however, intakes of several plant-based foods, especially whole grain, dry beans, and certain vegetables that provided dietary fiber, plant protein, and B vitamins, were associated with acetate, propionate, butyrate, and total SCFA production during inulin fermentation.
26583778	8	104	theme	gut	2159:2161	arg1	functionality					2174:2186	gut microbiota functionality	2159:2186	gut microbiota functionality	2159:2186	These results may help in the development of new dietary strategies to improve gut microbiota functionality to promote human health.
26583778	7	105	theme	BCFA	2062:2065	arg1	metabolism					2050:2059	detrimental metabolism	2038:2059	detrimental metabolism (BCFA production)	2038:2077	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	7	105	theme	BCFA	2062:2065	arg1	production					2067:2076	BCFA production	2062:2076	BCFA production	2062:2076	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	4	106	theme	one-year	1076:1083	arg1	records					1093:1099	one-year dietary records	1076:1099	one-year dietary records	1076:1099	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	1	107	theme	metabolic	193:201	arg1	activity					203:210	metabolic activity	193:210	metabolic activity	193:210	Although the composition of the gut microbiota is of interest, the functionality, or metabolic activity, of the gut microbiota is of equal importance: the gut microbiota can produce either harmful metabolites associated with human disease or beneficial metabolites that protect against disease.
26583778	7	108	theme	pronounced	1919:1928	arg1	effect					1930:1935	a pronounced effect	1917:1935	a pronounced effect	1917:1935	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	7	109	theme	beneficial	1991:2000	arg1	metabolism					2002:2011	beneficial metabolism	1991:2011	beneficial metabolism (butyrate production)	1991:2033	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	7	109	theme	beneficial	1991:2000	arg1	production					2023:2032	butyrate production	2014:2032	butyrate production	2014:2032	Comparing results between inulin and whole wheat fermentations, significant correlations were only found for butyrate and BCFA, suggesting that regardless of the type of carbohydrate provided to the microbiota, long-term diet may have a pronounced effect on the propensity of the gut microbiota toward either beneficial metabolism (butyrate production) or detrimental metabolism (BCFA production).
26583778	4	110	from	individuals	1045:1055	arg1	substrate					1178:1186	substrate	1178:1186	substrate	1178:1186	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
26583778	4	110	from	individuals	1045:1055	arg1	samples					1023:1029	Stool samples	1017:1029	Stool samples from eighteen individuals that had completed one-year dietary records	1017:1099	Stool samples from eighteen individuals that had completed one-year dietary records were used in an in vitro fecal fermentation system with long-chain inulin as substrate.
28961197	1	0	theme	related	356:362	arg1	diseases					375:382	related infectious diseases	356:382	related infectious diseases treatment	356:392	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	6	1	from	system	1227:1232	arg1	treatment					1241:1249	the treatment	1237:1249	the treatment of oral infectious diseases such as periodontitis and periodontitis	1237:1317	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	6	2	theme	delivery	1218:1225	arg1	system					1227:1232	an effective drug delivery system	1200:1232	an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft	1200:1345	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	2	3	theme	KSL/PLGA/CS	523:533	arg1	microspheres					509:520	KSL-W-loaded PLGA/chitosan composite microspheres	472:520	KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs)	472:538	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	2	3	theme	KSL/PLGA/CS	523:533	arg1	MSs					535:537	KSL/PLGA/CS MSs	523:537	KSL/PLGA/CS MSs	523:537	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	1	4	theme	infectious	364:373	arg1	diseases					375:382	related infectious diseases	356:382	related infectious diseases treatment	356:392	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	4	5	theme	KSL/PLGA/CS	889:899	arg1	MSs					901:903	KSL/PLGA/CS MSs	889:903	KSL/PLGA/CS MSs	889:903	Antibacterial experiment demonstrated the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria.
28961197	1	6	theme	biofilm	279:285	arg1	formation					287:295	biofilm formation	279:295	biofilm formation on teeth	279:304	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	1	7	theme	diseases	375:382	arg1	treatment					384:392	related infectious diseases treatment	356:392	related infectious diseases treatment	356:392	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	4	8	from	effects	878:884	arg1	bacteria					913:920	oral bacteria	908:920	oral bacteria	908:920	Antibacterial experiment demonstrated the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria.
28961197	4	9	theme	inhibitory	867:876	arg1	effects					878:884	the prolonged antimicrobial and inhibitory effects	835:884	the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria	835:920	Antibacterial experiment demonstrated the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria.
28961197	5	10	theme	MC3T3-E1	1062:1069	arg1	growth					1041:1046	the growth	1037:1046	the growth of osteoblast MC3T3-E1	1037:1069	Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1.
28961197	2	11	theme	electrospraying	557:571	arg1	methods					608:614	electrospraying and combined crosslinking-emulsion methods	557:614	electrospraying and combined crosslinking-emulsion methods	557:614	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	5	12	theme	antibacterial	993:1005	arg1	dosage					1007:1012	the released KSL-W antibacterial dosage	974:1012	the released KSL-W antibacterial dosage	974:1012	Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1.
28961197	6	13	contain	have	1155:1158	arg2	applications					1184:1195	potentially therapeutic applications	1160:1195	potentially therapeutic applications	1160:1195	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	6	13	contain	have	1155:1158	arg1	microspheres					1138:1149	the KSL-W-loaded PLGA/CS composite microspheres	1103:1149	the KSL-W-loaded PLGA/CS composite microspheres	1103:1149	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	6	14	theme	effective	1203:1211	arg1	system					1227:1232	an effective drug delivery system	1200:1232	an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft	1200:1345	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	1	15	theme	stable	196:201	arg1	activity					217:224	stable antimicrobial activity	196:224	stable antimicrobial activity	196:224	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	2	16	theme	PLGA/chitosan	485:497	arg1	microspheres					509:520	KSL-W-loaded PLGA/chitosan composite microspheres	472:520	KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs)	472:538	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	2	16	theme	PLGA/chitosan	485:497	arg1	MSs					535:537	KSL/PLGA/CS MSs	523:537	KSL/PLGA/CS MSs	523:537	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	6	17	theme	diseases	1270:1277	arg1	treatment					1241:1249	the treatment	1237:1249	the treatment of oral infectious diseases such as periodontitis and periodontitis	1237:1317	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	6	18	theme	drug	1213:1216	arg1	system					1227:1232	an effective drug delivery system	1200:1232	an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft	1200:1345	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	1	19	theme	antimicrobial	203:215	arg1	activity					217:224	stable antimicrobial activity	196:224	stable antimicrobial activity	196:224	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	2	20	theme	KSL-W-loaded	472:483	arg1	microspheres					509:520	KSL-W-loaded PLGA/chitosan composite microspheres	472:520	KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs)	472:538	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	2	20	theme	KSL-W-loaded	472:483	arg1	MSs					535:537	KSL/PLGA/CS MSs	523:537	KSL/PLGA/CS MSs	523:537	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	5	21	theme	osteoblast	1051:1060	arg1	MC3T3-E1					1062:1069	osteoblast MC3T3-E1	1051:1069	osteoblast MC3T3-E1	1051:1069	Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1.
28961197	6	22	theme	bone	1372:1375	arg1	augmentation					1377:1388	alveolar bone augmentation	1363:1388	alveolar bone augmentation	1363:1388	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	1	23	theme	antimicrobial	122:134	arg1	KSL-W					149:153	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂)	119:172	KSL-W	149:153	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	5	24	theme	released	978:985	arg1	dosage					1007:1012	the released KSL-W antibacterial dosage	974:1012	the released KSL-W antibacterial dosage	974:1012	Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1.
28961197	0	25	theme	Peptide-Loaded	29:42	arg1	Microspheres					68:79	Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres	15:79	Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres	15:79	Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance.
28961197	4	26	theme	oral	908:911	arg1	bacteria					913:920	oral bacteria	908:920	oral bacteria	908:920	Antibacterial experiment demonstrated the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria.
28961197	1	27	from	formation	287:295	arg1	teeth					300:304	teeth	300:304	teeth	300:304	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	1	28	theme	decapeptide	136:146	arg1	KSL-W					149:153	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂)	119:172	KSL-W	149:153	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	2	29	theme	long-term	440:448	arg1	resistance					460:469	long-term bacterial resistance	440:469	long-term bacterial resistance	440:469	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	0	30	theme	Antimicrobial	15:27	arg1	Microspheres					68:79	Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres	15:79	Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres	15:79	Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance.
28961197	3	31	theme	encapsulation	720:732	arg1	efficiency					734:743	encapsulation efficiency	720:743	encapsulation efficiency	720:743	Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity.
28961197	4	32	theme	prolonged	839:847	arg1	effects					878:884	the prolonged antimicrobial and inhibitory effects	835:884	the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria	835:920	Antibacterial experiment demonstrated the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria.
28961197	5	33	theme	cell	937:940	arg1	assay					956:960	the cell proliferation assay	933:960	the cell proliferation assay	933:960	Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1.
28961197	0	34	theme	Composite	58:66	arg1	Microspheres					68:79	Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres	15:79	Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres	15:79	Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance.
28961197	2	35	theme	crosslinking-emulsion	586:606	arg1	methods					608:614	electrospraying and combined crosslinking-emulsion methods	557:614	electrospraying and combined crosslinking-emulsion methods	557:614	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	5	36	theme	proliferation	942:954	arg1	assay					956:960	the cell proliferation assay	933:960	the cell proliferation assay	933:960	Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1.
28961197	6	37	theme	alveolar	1363:1370	arg1	augmentation					1377:1388	alveolar bone augmentation	1363:1388	alveolar bone augmentation	1363:1388	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	2	38	theme	KSL-W	430:434	arg1	release					419:425	the release	415:425	the release of KSL-W for long-term bacterial resistance	415:469	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	0	39	theme	PLGA/Chitosan	44:56	arg1	Microspheres					68:79	Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres	15:79	Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres	15:79	Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance.
28961197	2	40	theme	combined	577:584	arg1	methods					608:614	electrospraying and combined crosslinking-emulsion methods	557:614	electrospraying and combined crosslinking-emulsion methods	557:614	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	4	41	theme	antimicrobial	849:861	arg1	effects					878:884	the prolonged antimicrobial and inhibitory effects	835:884	the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria	835:920	Antibacterial experiment demonstrated the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria.
28961197	2	42	theme	composite	499:507	arg1	microspheres					509:520	KSL-W-loaded PLGA/chitosan composite microspheres	472:520	KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs)	472:538	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	2	42	theme	composite	499:507	arg1	MSs					535:537	KSL/PLGA/CS MSs	523:537	KSL/PLGA/CS MSs	523:537	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	6	43	theme	bone	1336:1339	arg1	graft					1341:1345	bone graft	1336:1345	bone graft	1336:1345	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	6	44	theme	oral	1254:1257	arg1	periodontitis					1305:1317	periodontitis	1305:1317	periodontitis	1305:1317	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	6	44	theme	oral	1254:1257	arg1	diseases					1270:1277	oral infectious diseases	1254:1277	oral infectious diseases such as periodontitis and periodontitis	1254:1317	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	6	44	theme	oral	1254:1257	arg1	periodontitis					1287:1299	periodontitis	1287:1299	periodontitis	1287:1299	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	0	45	theme	Microspheres	68:79	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance.	0:117	Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance.
28961197	1	46	theme	oral	332:335	arg1	microorganisms					337:350	oral microorganisms	332:350	oral microorganisms for related infectious diseases treatment	332:392	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	3	47	theme	drug	755:758	arg1	release					760:766	in vitro drug release	746:766	in vitro drug release	746:766	Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity.
28961197	1	48	theme	microorganisms	337:350	arg1	growth					322:327	the growth	318:327	the growth of oral microorganisms for related infectious diseases treatment	318:392	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	1	49	used	used	263:266	arg2	KSL-W					149:153	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂)	119:172	KSL-W	149:153	An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH₂), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment.
28961197	4	50	theme	MSs	901:903	arg1	effects					878:884	the prolonged antimicrobial and inhibitory effects	835:884	the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria	835:920	Antibacterial experiment demonstrated the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria.
28961197	3	51	theme	Different	617:625	arg1	formulations					627:638	Different formulations	617:638	Different formulations of microspheres	617:654	Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity.
28961197	3	52	dep	in	746:747	arg1	vitro					749:753	vitro	749:753	vitro	749:753	Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity.
28961197	3	53	theme	size	701:704	arg1	distribution					706:717	size distribution	701:717	size distribution	701:717	Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity.
28961197	5	54	contain	had	1014:1016	arg1	dosage					1007:1012	the released KSL-W antibacterial dosage	974:1012	the released KSL-W antibacterial dosage	974:1012	Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1.
28961197	5	54	contain	had	1014:1016	arg2	cytotoxicity					1021:1032	no cytotoxicity	1018:1032	no cytotoxicity	1018:1032	Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1.
28961197	4	55	theme	Antibacterial	797:809	arg1	experiment					811:820	Antibacterial experiment	797:820	Antibacterial experiment	797:820	Antibacterial experiment demonstrated the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria.
28961197	6	56	theme	composite	1128:1136	arg1	microspheres					1138:1149	the KSL-W-loaded PLGA/CS composite microspheres	1103:1149	the KSL-W-loaded PLGA/CS composite microspheres	1103:1149	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	0	57	theme	Bacterial	97:105	arg1	Resistance					107:116	Long-Acting Bacterial Resistance	85:116	Long-Acting Bacterial Resistance	85:116	Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance.
28961197	3	58	theme	in	746:747	arg1	release					760:766	in vitro drug release	746:766	in vitro drug release	746:766	Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity.
28961197	3	59	theme	surface	681:687	arg1	morphology					689:698	surface morphology	681:698	surface morphology	681:698	Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity.
28961197	6	60	theme	therapeutic	1172:1182	arg1	applications					1184:1195	potentially therapeutic applications	1160:1195	potentially therapeutic applications	1160:1195	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	5	61	theme	KSL-W	987:991	arg1	dosage					1007:1012	the released KSL-W antibacterial dosage	974:1012	the released KSL-W antibacterial dosage	974:1012	Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1.
28961197	6	62	theme	PLGA/CS	1120:1126	arg1	microspheres					1138:1149	the KSL-W-loaded PLGA/CS composite microspheres	1103:1149	the KSL-W-loaded PLGA/CS composite microspheres	1103:1149	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	0	63	theme	Long-Acting	85:95	arg1	Resistance					107:116	Long-Acting Bacterial Resistance	85:116	Long-Acting Bacterial Resistance	85:116	Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance.
28961197	6	64	theme	infectious	1259:1268	arg1	periodontitis					1305:1317	periodontitis	1305:1317	periodontitis	1305:1317	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	6	64	theme	infectious	1259:1268	arg1	diseases					1270:1277	oral infectious diseases	1254:1277	oral infectious diseases such as periodontitis and periodontitis	1254:1317	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	6	64	theme	infectious	1259:1268	arg1	periodontitis					1287:1299	periodontitis	1287:1299	periodontitis	1287:1299	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
28961197	2	65	theme	bacterial	450:458	arg1	resistance					460:469	long-term bacterial resistance	440:469	long-term bacterial resistance	440:469	In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods.
28961197	3	66	theme	antimicrobial	773:785	arg1	activity					787:794	antimicrobial activity	773:794	antimicrobial activity	773:794	Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity.
28961197	3	67	theme	microspheres	643:654	arg1	formulations					627:638	Different formulations	617:638	Different formulations of microspheres	617:654	Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity.
28961197	6	68	theme	KSL-W-loaded	1107:1118	arg1	microspheres					1138:1149	the KSL-W-loaded PLGA/CS composite microspheres	1103:1149	the KSL-W-loaded PLGA/CS composite microspheres	1103:1149	Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
27960600	4	0	theme	intraparticle	820:832	arg1	diffusion					834:842	intraparticle diffusion	820:842	intraparticle diffusion	820:842	In addition, the encapsulating matrix introduces supplementary resistance to intraparticle diffusion, especially when the resin is dried without control: freeze-drying partially limits this effect.
27960600	3	1	theme	kinetic	663:669	arg1	profiles					671:678	the kinetic profiles	659:678	the kinetic profiles	659:678	Sorption isotherms are modeled using the Langmuir equation, while the kinetic profiles are presented a good fit by pseudo-second order rate equation.
27960600	1	2	dep	boron	254:258	arg1	capacity					270:277	sorption capacity	261:277	sorption capacity close to 0.89 mmol B g-1	261:302	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	2	3	theme	alginate	542:549	arg1	introduction					526:537	the introduction	522:537	the introduction of alginate having poor efficiency for boron uptake	522:589	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	1	4	theme	efficient	232:240	arg1	sorption					242:249	an efficient sorption	229:249	an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1)	229:303	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	0	5	theme	hydrogel	102:109	arg1	beads					111:115	alginate hydrogel beads	93:115	alginate hydrogel beads	93:115	Boron removal by a composite sorbent: Polyethylenimine/tannic acid derivative immobilized in alginate hydrogel beads.
27960600	3	6	theme	Langmuir	634:641	arg1	equation					643:650	the Langmuir equation	630:650	the Langmuir equation	630:650	Sorption isotherms are modeled using the Langmuir equation, while the kinetic profiles are presented a good fit by pseudo-second order rate equation.
27960600	4	7	theme	encapsulating	760:772	arg1	matrix					774:779	the encapsulating matrix	756:779	the encapsulating matrix	756:779	In addition, the encapsulating matrix introduces supplementary resistance to intraparticle diffusion, especially when the resin is dried without control: freeze-drying partially limits this effect.
27960600	0	8	theme	alginate	93:100	arg1	beads					111:115	alginate hydrogel beads	93:115	alginate hydrogel beads	93:115	Boron removal by a composite sorbent: Polyethylenimine/tannic acid derivative immobilized in alginate hydrogel beads.
27960600	2	9	from	use	421:423	arg1	columns					438:444	fixed-bed columns	428:444	fixed-bed columns	428:444	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	7	10	with	ion-exchange	1147:1158	arg1	I					1178:1178	I	1178:1178	I	1178:1178	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I)) leads to a decrease in the sorption performance of composite material but the action of Ca(II) ions in the solutions re-stabilizes the hydrogel.
27960600	7	10	with	ion-exchange	1147:1158	arg1	Na					1175:1176	Na	1175:1176	Na(I)	1175:1179	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I)) leads to a decrease in the sorption performance of composite material but the action of Ca(II) ions in the solutions re-stabilizes the hydrogel.
27960600	7	11	from	action	1260:1265	arg1	solutions					1289:1297	the solutions	1285:1297	the solutions	1285:1297	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I)) leads to a decrease in the sorption performance of composite material but the action of Ca(II) ions in the solutions re-stabilizes the hydrogel.
27960600	5	12	theme	nitrogen	1038:1045	arg1	atmosphere					1047:1056	nitrogen atmosphere	1038:1056	nitrogen atmosphere	1038:1056	The stability (at long-term storage) of the sorbent is improved when the sorbent is stored under nitrogen atmosphere.
27960600	2	13	theme	boron	578:582	arg1	uptake					584:589	boron uptake	578:589	boron uptake	578:589	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	7	14	theme	ions	1277:1280	arg1	action					1260:1265	the action	1256:1265	the action of Ca(II) ions in the solutions	1256:1297	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I)) leads to a decrease in the sorption performance of composite material but the action of Ca(II) ions in the solutions re-stabilizes the hydrogel.
27960600	2	15	theme	crushed	358:364	arg1	sorbent					342:348	this chelating sorbent	327:348	this chelating sorbent (finely crushed)	327:365	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	7	16	theme	hydrogel	1134:1141	arg1	degradation					1115:1125	The degradation	1111:1125	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I))	1111:1180	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I)) leads to a decrease in the sorption performance of composite material but the action of Ca(II) ions in the solutions re-stabilizes the hydrogel.
27960600	2	17	theme	solid/liquid	396:407	arg1	use					421:423	use	421:423	use in fixed-bed columns	421:444	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	2	17	theme	solid/liquid	396:407	arg1	separation					409:418	readily solid/liquid separation	388:418	readily solid/liquid separation	388:418	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	7	18	theme	sorption	1209:1216	arg1	performance					1218:1228	the sorption performance	1205:1228	the sorption performance of composite material	1205:1250	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I)) leads to a decrease in the sorption performance of composite material but the action of Ca(II) ions in the solutions re-stabilizes the hydrogel.
27960600	1	19	theme	tannic	177:182	arg1	acid					184:187	tannic acid	177:187	tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1)	177:303	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	0	20	theme	Boron	0:4	arg1	removal					6:12	Boron removal	0:12	Boron removal by a composite sorbent: Polyethylenimine/tannic acid derivative immobilized in alginate hydrogel beads.	0:116	Boron removal by a composite sorbent: Polyethylenimine/tannic acid derivative immobilized in alginate hydrogel beads.
27960600	1	21	theme	boron	254:258	arg1	sorption					242:249	an efficient sorption	229:249	an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1)	229:303	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	2	22	theme	poor	558:561	arg1	efficiency					563:572	poor efficiency	558:572	poor efficiency for boron uptake	558:589	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	6	23	theme	NaCl	1088:1091	arg1	excess					1078:1083	an excess	1075:1083	an excess of NaCl	1075:1091	The presence of an excess of NaCl was investigated.
27960600	3	24	theme	order	722:726	arg1	equation					733:740	pseudo-second order rate equation	708:740	pseudo-second order rate equation	708:740	Sorption isotherms are modeled using the Langmuir equation, while the kinetic profiles are presented a good fit by pseudo-second order rate equation.
27960600	1	25	theme	acid	184:187	arg1	grafting					165:172	the grafting	161:172	the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1)	161:303	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	3	26	theme	good	696:699	arg1	fit					701:703	a good fit	694:703	a good fit	694:703	Sorption isotherms are modeled using the Langmuir equation, while the kinetic profiles are presented a good fit by pseudo-second order rate equation.
27960600	7	27	theme	Ca	1270:1271	arg1	ions					1277:1280	Ca(II) ions	1270:1280	Ca(II) ions	1270:1280	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I)) leads to a decrease in the sorption performance of composite material but the action of Ca(II) ions in the solutions re-stabilizes the hydrogel.
27960600	1	28	theme	sorption	261:268	arg1	capacity					270:277	sorption capacity	261:277	sorption capacity close to 0.89 mmol B g-1	261:302	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	6	29	theme	excess	1078:1083	arg1	presence					1063:1070	The presence	1059:1070	The presence of an excess of NaCl	1059:1091	The presence of an excess of NaCl was investigated.
27960600	5	30	from	storage	969:975	arg1	stability					945:953	The stability	941:953	The stability (at long-term storage) of the sorbent	941:991	The stability (at long-term storage) of the sorbent is improved when the sorbent is stored under nitrogen atmosphere.
27960600	2	31	theme	fixed-bed	428:436	arg1	columns					438:444	fixed-bed columns	428:444	fixed-bed columns	428:444	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	2	32	theme	sorption	475:482	arg1	capacity					484:491	sorption capacity	475:491	sorption capacity	475:491	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	3	33	theme	Sorption	593:600	arg1	isotherms					602:610	Sorption isotherms	593:610	Sorption isotherms	593:610	Sorption isotherms are modeled using the Langmuir equation, while the kinetic profiles are presented a good fit by pseudo-second order rate equation.
27960600	2	34	theme	sorbent	342:348	arg1	encapsulation					310:322	The encapsulation	306:322	The encapsulation of this chelating sorbent (finely crushed)	306:365	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	0	35	theme	composite	19:27	arg1	sorbent					29:35	a composite sorbent	17:35	a composite sorbent	17:35	Boron removal by a composite sorbent: Polyethylenimine/tannic acid derivative immobilized in alginate hydrogel beads.
27960600	3	36	theme	pseudo-second	708:720	arg1	equation					733:740	pseudo-second order rate equation	708:740	pseudo-second order rate equation	708:740	Sorption isotherms are modeled using the Langmuir equation, while the kinetic profiles are presented a good fit by pseudo-second order rate equation.
27960600	0	37	theme	Polyethylenimine/tannic	38:60	arg1	derivative					67:76	Polyethylenimine/tannic acid derivative	38:76	Boron removal by a composite sorbent: Polyethylenimine/tannic acid derivative immobilized in alginate hydrogel beads.	0:116	Boron removal by a composite sorbent: Polyethylenimine/tannic acid derivative immobilized in alginate hydrogel beads.
27960600	2	38	theme	loss	467:470	arg1	expense					454:460	the expense	450:460	the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake)	450:590	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	7	39	theme	Ca	1163:1164	arg1	ion-exchange					1147:1158	ion-exchange	1147:1158	ion-exchange of Ca(II) with Na(I)	1147:1179	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I)) leads to a decrease in the sorption performance of composite material but the action of Ca(II) ions in the solutions re-stabilizes the hydrogel.
27960600	2	40	dep	use	383:385	arg1	use					421:423	use	421:423	use in fixed-bed columns	421:444	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	2	40	dep	use	383:385	arg1	separation					409:418	readily solid/liquid separation	388:418	readily solid/liquid separation	388:418	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	4	41	theme	supplementary	792:804	arg1	resistance					806:815	supplementary resistance	792:815	supplementary resistance to intraparticle diffusion	792:842	In addition, the encapsulating matrix introduces supplementary resistance to intraparticle diffusion, especially when the resin is dried without control: freeze-drying partially limits this effect.
27960600	1	42	theme	0.89 mmol	288:296	arg1	g-1					300:302	0.89 mmol B g-1	288:302	0.89 mmol B g-1	288:302	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	5	43	theme	sorbent	985:991	arg1	stability					945:953	The stability	941:953	The stability (at long-term storage) of the sorbent	941:991	The stability (at long-term storage) of the sorbent is improved when the sorbent is stored under nitrogen atmosphere.
27960600	1	44	theme	novel	120:124	arg1	material					136:143	A novel composite material	118:143	A novel composite material	118:143	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	1	45	theme	B	298:298	arg1	g-1					300:302	0.89 mmol B g-1	288:302	0.89 mmol B g-1	288:302	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	2	46	from	expense	454:460	arg1	capacity					484:491	sorption capacity	475:491	sorption capacity	475:491	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	7	47	theme	composite	1233:1241	arg1	material					1243:1250	composite material	1233:1250	composite material	1233:1250	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I)) leads to a decrease in the sorption performance of composite material but the action of Ca(II) ions in the solutions re-stabilizes the hydrogel.
27960600	1	48	from	polyethylenimine	192:207	arg1	grafting					165:172	the grafting	161:172	the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1)	161:303	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	1	49	theme	composite	126:134	arg1	material					136:143	A novel composite material	118:143	A novel composite material	118:143	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	2	50	from	loss	467:470	arg1	capacity					484:491	sorption capacity	475:491	sorption capacity	475:491	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	7	51	theme	material	1243:1250	arg1	performance					1218:1228	the sorption performance	1205:1228	the sorption performance of composite material	1205:1250	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I)) leads to a decrease in the sorption performance of composite material but the action of Ca(II) ions in the solutions re-stabilizes the hydrogel.
27960600	7	52	from	decrease	1193:1200	arg1	performance					1218:1228	the sorption performance	1205:1228	the sorption performance of composite material	1205:1250	The degradation of the hydrogel (by ion-exchange of Ca(II) with Na(I)) leads to a decrease in the sorption performance of composite material but the action of Ca(II) ions in the solutions re-stabilizes the hydrogel.
27960600	0	53	dep	removal	6:12	arg1	derivative					67:76	Polyethylenimine/tannic acid derivative	38:76	Boron removal by a composite sorbent: Polyethylenimine/tannic acid derivative immobilized in alginate hydrogel beads.	0:116	Boron removal by a composite sorbent: Polyethylenimine/tannic acid derivative immobilized in alginate hydrogel beads.
27960600	1	54	from	acid	184:187	arg1	polyethylenimine					192:207	polyethylenimine	192:207	polyethylenimine (PEI)	192:213	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	1	54	from	acid	184:187	arg1	PEI					210:212	PEI	210:212	PEI	210:212	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	2	55	theme	chelating	332:340	arg1	sorbent					342:348	this chelating sorbent	327:348	this chelating sorbent (finely crushed)	327:365	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	2	56	contain	having	551:556	arg1	alginate					542:549	alginate	542:549	alginate having poor efficiency for boron uptake	542:589	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	2	56	contain	having	551:556	arg2	efficiency					563:572	poor efficiency	558:572	poor efficiency for boron uptake	558:589	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
27960600	3	57	theme	rate	728:731	arg1	equation					733:740	pseudo-second order rate equation	708:740	pseudo-second order rate equation	708:740	Sorption isotherms are modeled using the Langmuir equation, while the kinetic profiles are presented a good fit by pseudo-second order rate equation.
27960600	0	58	theme	acid	62:65	arg1	derivative					67:76	Polyethylenimine/tannic acid derivative	38:76	Boron removal by a composite sorbent: Polyethylenimine/tannic acid derivative immobilized in alginate hydrogel beads.	0:116	Boron removal by a composite sorbent: Polyethylenimine/tannic acid derivative immobilized in alginate hydrogel beads.
27960600	5	59	theme	long-term	959:967	arg1	storage					969:975	long-term storage	959:975	long-term storage	959:975	The stability (at long-term storage) of the sorbent is improved when the sorbent is stored under nitrogen atmosphere.
27960600	1	60	from	grafting	165:172	arg1	polyethylenimine					192:207	polyethylenimine	192:207	polyethylenimine (PEI)	192:213	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	1	60	from	grafting	165:172	arg1	PEI					210:212	PEI	210:212	PEI	210:212	A novel composite material was prepared by the grafting of tannic acid on polyethylenimine (PEI), which allows an efficient sorption of boron (sorption capacity close to 0.89 mmol B g-1).
27960600	2	61	from	capacity	484:491	arg1	expense					454:460	the expense	450:460	the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake)	450:590	The encapsulation of this chelating sorbent (finely crushed) facilitates its use (readily solid/liquid separation, use in fixed-bed columns) at the expense of a loss in sorption capacity (proportionally decreased by the introduction of alginate having poor efficiency for boron uptake).
28346394	4	0	theme	mouse	768:772	arg1	model					784:788	an in vivo pancreatic cancer mouse xenograft model	739:788	an in vivo pancreatic cancer mouse xenograft model	739:788	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	6	1	theme	Pancreatic	983:992	arg1	mice					1011:1014	Pancreatic cancer xenograft mice	983:1014	Pancreatic cancer xenograft mice	983:1014	Pancreatic cancer xenograft mice were subjected to an ERS diet to assess tumor volume and weight as compared to mice fed with a control diet.
28346394	10	2	theme	formate	1804:1810	arg1	growth					1752:1757	enhanced growth	1743:1757	enhanced growth on residual substrates	1743:1780	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	2	theme	formate	1804:1810	arg1	amounts					1839:1845	decreased amounts	1829:1845	decreased amounts of propionate	1829:1859	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	2	theme	formate	1804:1810	arg1	formate					1804:1810	formate	1804:1810	formate	1804:1810	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	2	theme	formate	1804:1810	arg1	lactate					1816:1822	lactate	1816:1822	lactate	1816:1822	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	2	theme	formate	1804:1810	arg1	propionate					1850:1859	propionate	1850:1859	propionate	1850:1859	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	2	theme	formate	1804:1810	arg1	production					1790:1799	higher production	1783:1799	higher production of formate and lactate	1783:1822	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	0	3	theme	Pancreatic	144:153	arg1	Models					162:167	In Vitro and In Vivo Pancreatic Cancer Models	123:167	In Vitro and In Vivo Pancreatic Cancer Models	123:167	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	0	4	with	Parallel	74:81	arg1	Retardation					92:102	the Retardation	88:102	the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models	88:167	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	8	5	dep	phospho-mTOR	1418:1429	arg1	target					1442:1447	mammalian target	1432:1447	mammalian target of rapamycin	1432:1460	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	2	6	from	advances	298:305	arg1	options					320:326	treatment options	310:326	treatment options	310:326	Despite recent advances in treatment options, a modest impact on the outcome of the disease is observed so far.
28346394	4	7	theme	study	545:549	arg1	aim					533:535	The aim	529:535	The aim of this study	529:549	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	12	8	from	efficacy	2259:2266	arg1	patients					2319:2326	pancreatic cancer patients	2301:2326	pancreatic cancer patients	2301:2326	These results suggest that engineered dietary interventions could be supportive as a synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients.
28346394	3	9	theme	chemotherapy	504:515	arg1	efficacy					492:499	the efficacy	488:499	the efficacy of chemotherapy against PC	488:526	We have previously demonstrated that short-term fasting cycles have the potential to improve the efficacy of chemotherapy against PC.
28346394	1	10	theme	leading	233:239	arg1	cause					241:245	the fourth leading cause	222:245	the fourth leading cause of cancer-related deaths worldwide	222:280	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	1	10	theme	leading	233:239	arg1	cancer					197:202	BACKGROUND/AIMS Pancreatic cancer	170:202	BACKGROUND/AIMS Pancreatic cancer (PC)	170:207	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	10	11	from	production	1790:1799	arg1	substrates					1771:1780	residual substrates	1762:1780	residual substrates	1762:1780	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	4	12	theme	in	742:743	arg1	model					784:788	an in vivo pancreatic cancer mouse xenograft model	739:788	an in vivo pancreatic cancer mouse xenograft model	739:788	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	6	13	theme	tumor	1056:1060	arg1	volume					1062:1067	tumor volume	1056:1067	tumor volume	1056:1067	Pancreatic cancer xenograft mice were subjected to an ERS diet to assess tumor volume and weight as compared to mice fed with a control diet.
28346394	4	14	theme	pancreatic	750:759	arg1	model					784:788	an in vivo pancreatic cancer mouse xenograft model	739:788	an in vivo pancreatic cancer mouse xenograft model	739:788	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	11	15	from	decrease	2067:2074	arg1	model					2131:2135	the in vivo PC xenograft mouse model	2100:2135	the in vivo PC xenograft mouse model	2100:2135	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	6	16	theme	control	1111:1117	arg1	diet					1119:1122	a control diet	1109:1122	a control diet	1109:1122	Pancreatic cancer xenograft mice were subjected to an ERS diet to assess tumor volume and weight as compared to mice fed with a control diet.
28346394	10	17	theme	microbial	1686:1694	arg1	cultures					1696:1703	the fecal microbial cultures	1676:1703	the fecal microbial cultures from mice fed with an ERS diet	1676:1734	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	8	18	dep	RESULTS	1250:1256	arg1	showed					1323:1328	showed	1323:1328	showed	1323:1328	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	9	19	theme	significant	1603:1613	arg1	retardation					1615:1625	significant retardation	1603:1625	significant retardation in tumor growth	1603:1641	Consistently, xenograft pancreatic cancer mice subjected to an ERS diet displayed significant retardation in tumor growth.
28346394	6	20	theme	ERS	1037:1039	arg1	diet					1041:1044	an ERS diet	1034:1044	an ERS diet	1034:1044	Pancreatic cancer xenograft mice were subjected to an ERS diet to assess tumor volume and weight as compared to mice fed with a control diet.
28346394	11	21	theme	mouse	2006:2010	arg1	microbiota					2018:2027	mouse fecal microbiota	2006:2027	mouse fecal microbiota shown in vitro	2006:2042	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	10	22	theme	propionate	1850:1859	arg1	growth					1752:1757	enhanced growth	1743:1757	enhanced growth on residual substrates	1743:1780	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	22	theme	propionate	1850:1859	arg1	amounts					1839:1845	decreased amounts	1829:1845	decreased amounts of propionate	1829:1859	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	22	theme	propionate	1850:1859	arg1	formate					1804:1810	formate	1804:1810	formate	1804:1810	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	22	theme	propionate	1850:1859	arg1	lactate					1816:1822	lactate	1816:1822	lactate	1816:1822	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	22	theme	propionate	1850:1859	arg1	propionate					1850:1859	propionate	1850:1859	propionate	1850:1859	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	22	theme	propionate	1850:1859	arg1	production					1790:1799	higher production	1783:1799	higher production of formate and lactate	1783:1822	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	0	23	theme	Tumor	107:111	arg1	Growth					113:118	Tumor Growth	107:118	Tumor Growth	107:118	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	5	24	theme	growth	948:953	arg1	pathways					973:980	tumor growth and proliferation pathways	942:980	tumor growth and proliferation pathways	942:980	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	1	25	theme	Pancreatic	186:195	arg1	PC					205:206	PC	205:206	PC	205:206	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	1	25	theme	Pancreatic	186:195	arg1	cancer					197:202	BACKGROUND/AIMS Pancreatic cancer	170:202	BACKGROUND/AIMS Pancreatic cancer (PC)	170:207	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	1	25	theme	Pancreatic	186:195	arg1	cause					241:245	the fourth leading cause	222:245	the fourth leading cause of cancer-related deaths worldwide	222:280	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	4	26	theme	engineered	582:591	arg1	ERS					611:613	ERS	611:613	ERS	611:613	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	4	26	theme	engineered	582:591	arg1	resistant-starch					593:608	an engineered resistant-starch	579:608	an engineered resistant-starch (ERS) mimicking diet	579:629	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	5	27	theme	proliferation	959:971	arg1	pathways					973:980	tumor growth and proliferation pathways	942:980	tumor growth and proliferation pathways	942:980	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	10	28	from	amounts	1839:1845	arg1	substrates					1771:1780	residual substrates	1762:1780	residual substrates	1762:1780	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	11	29	theme	diet	1968:1971	arg1	effect					1950:1955	A positive effect	1939:1955	A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro	1939:2042	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	8	30	theme	phospho-ERK1/2	1350:1363	arg1	levels					1463:1468	phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels	1350:1468	phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels	1350:1468	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	10	31	theme	fecal	1874:1878	arg1	microbiota					1880:1889	fecal microbiota	1874:1889	fecal microbiota from mice fed with the control diet	1874:1925	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	0	32	from	Retardation	92:102	arg1	Models					162:167	In Vitro and In Vivo Pancreatic Cancer Models	123:167	In Vitro and In Vivo Pancreatic Cancer Models	123:167	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	0	33	dep	In	136:137	arg1	Vivo					139:142	Vivo	139:142	Vivo	139:142	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	8	34	theme	extracellular	1366:1378	arg1	kinase					1397:1402	extracellular signal-regulated kinase	1366:1402	extracellular signal-regulated kinase proteins	1366:1411	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	4	35	theme	cell	655:658	arg1	lines					660:664	cancer cell lines	648:664	cancer cell lines	648:664	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	0	36	theme	In	136:137	arg1	Models					162:167	In Vitro and In Vivo Pancreatic Cancer Models	123:167	In Vitro and In Vivo Pancreatic Cancer Models	123:167	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	8	37	theme	kinase	1397:1402	arg1	phospho-ERK1/2					1350:1363	phospho-ERK1/2	1350:1363	phospho-ERK1/2 (extracellular signal-regulated kinase proteins)	1350:1412	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	8	37	theme	kinase	1397:1402	arg1	proteins					1404:1411	extracellular signal-regulated kinase proteins	1366:1411	extracellular signal-regulated kinase proteins	1366:1411	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	0	38	theme	Microbiota	52:61	arg1	Profile					63:69	Colon Microbiota Profile	46:69	Colon Microbiota Profile	46:69	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	11	39	theme	tumor	2079:2083	arg1	progression					2085:2095	tumor progression	2079:2095	tumor progression	2079:2095	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	11	40	dep	CONCLUSION	1928:1937	arg1	associated					2047:2056	associated	2047:2056	is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model	2044:2135	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	11	41	theme	PC	2112:2113	arg1	model					2131:2135	the in vivo PC xenograft mouse model	2100:2135	the in vivo PC xenograft mouse model	2100:2135	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	8	42	theme	phospho-mTOR	1418:1429	arg1	levels					1463:1468	phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels	1350:1468	phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels	1350:1468	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	5	43	dep	MATERIALS	791:799	arg1	cells					843:847	PANC-1 cells	836:847	PANC-1 cells	836:847	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	5	43	dep	MATERIALS	791:799	arg1	MATERIALS					791:799	MATERIALS	791:799	MATERIALS	791:799	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	5	43	dep	MATERIALS	791:799	arg1	BxPC-3					813:818	BxPC-3	813:818	BxPC-3	813:818	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	5	43	dep	MATERIALS	791:799	arg1	PaCa-2					825:830	MIA PaCa-2	821:830	MIA PaCa-2	821:830	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	11	44	from	effect	1950:1955	arg1	metabolism					1992:2001	metabolism	1992:2001	metabolism	1992:2001	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	11	44	from	effect	1950:1955	arg1	composition					1976:1986	composition	1976:1986	composition	1976:1986	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	10	45	from	mice	1710:1713	arg1	cultures					1696:1703	the fecal microbial cultures	1676:1703	the fecal microbial cultures from mice fed with an ERS diet	1676:1734	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	9	46	from	retardation	1615:1625	arg1	growth					1636:1641	tumor growth	1630:1641	tumor growth	1630:1641	Consistently, xenograft pancreatic cancer mice subjected to an ERS diet displayed significant retardation in tumor growth.
28346394	11	47	theme	in	2104:2105	arg1	model					2131:2135	the in vivo PC xenograft mouse model	2100:2135	the in vivo PC xenograft mouse model	2100:2135	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	8	48	theme	mammalian	1432:1440	arg1	target					1442:1447	mammalian target	1432:1447	mammalian target of rapamycin	1432:1460	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	10	49	dep	in	1647:1648	arg1	vitro					1650:1654	vitro	1650:1654	vitro	1650:1654	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	8	50	theme	Pancreatic	1258:1267	arg1	cells					1276:1280	Pancreatic cancer cells	1258:1280	Pancreatic cancer cells cultured in an ERS diet-mimicking medium	1258:1321	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	0	51	theme	Engineered	0:9	arg1	Diet					34:37	Engineered Resistant-Starch (ERS) Diet	0:37	Engineered Resistant-Starch (ERS) Diet	0:37	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	5	52	theme	PANC-1	836:841	arg1	cells					843:847	PANC-1 cells	836:847	PANC-1 cells	836:847	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	1	53	theme	cancer-related	250:263	arg1	worldwide					272:280	cancer-related deaths worldwide	250:280	cancer-related deaths worldwide	250:280	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	10	54	from	mice	1896:1899	arg1	microbiota					1880:1889	fecal microbiota	1874:1889	fecal microbiota from mice fed with the control diet	1874:1925	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	4	55	theme	tumor	723:727	arg1	growth					729:734	tumor growth	723:734	tumor growth in an in vivo pancreatic cancer mouse xenograft model	723:788	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	2	56	theme	disease	367:373	arg1	outcome					352:358	the outcome	348:358	the outcome of the disease	348:373	Despite recent advances in treatment options, a modest impact on the outcome of the disease is observed so far.
28346394	1	57	theme	worldwide	272:280	arg1	cause					241:245	the fourth leading cause	222:245	the fourth leading cause of cancer-related deaths worldwide	222:280	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	1	57	theme	worldwide	272:280	arg1	cancer					197:202	BACKGROUND/AIMS Pancreatic cancer	170:202	BACKGROUND/AIMS Pancreatic cancer (PC)	170:207	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	5	58	theme	diet	904:907	arg1	condition					919:927	the ERS-mimicking diet culturing condition	886:927	the ERS-mimicking diet culturing condition	886:927	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	7	59	theme	microbiota	1163:1172	arg1	activity					1145:1152	activity	1145:1152	activity	1145:1152	The composition and activity of fecal microbiota were further analyzed in growth experiments by isothermal microcalorimetry.
28346394	7	59	theme	microbiota	1163:1172	arg1	composition					1129:1139	composition	1129:1139	composition	1129:1139	The composition and activity of fecal microbiota were further analyzed in growth experiments by isothermal microcalorimetry.
28346394	12	60	theme	treatments	2287:2296	arg1	efficacy					2259:2266	the efficacy	2255:2266	the efficacy of existing cancer treatments in pancreatic cancer patients	2255:2326	These results suggest that engineered dietary interventions could be supportive as a synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients.
28346394	11	61	dep	in	2104:2105	arg1	vivo					2107:2110	vivo	2107:2110	vivo	2107:2110	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	10	62	theme	enhanced	1743:1750	arg1	growth					1752:1757	enhanced growth	1743:1757	enhanced growth on residual substrates	1743:1780	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	8	63	theme	ERS	1297:1299	arg1	medium					1316:1321	an ERS diet-mimicking medium	1294:1321	an ERS diet-mimicking medium	1294:1321	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	12	64	theme	pancreatic	2301:2310	arg1	patients					2319:2326	pancreatic cancer patients	2301:2326	pancreatic cancer patients	2301:2326	These results suggest that engineered dietary interventions could be supportive as a synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients.
28346394	10	65	theme	higher	1783:1788	arg1	production					1790:1799	higher production	1783:1799	higher production of formate and lactate	1783:1822	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	4	66	theme	microbiota	704:713	arg1	composition					683:693	the composition	679:693	the composition of fecal microbiota	679:713	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	3	67	theme	short-term	432:441	arg1	cycles					451:456	short-term fasting cycles	432:456	short-term fasting cycles	432:456	We have previously demonstrated that short-term fasting cycles have the potential to improve the efficacy of chemotherapy against PC.
28346394	8	68	theme	decreased	1330:1338	arg1	levels					1340:1345	decreased levels	1330:1345	decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels	1330:1468	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	10	69	theme	in	1647:1648	arg1	experiments					1663:1673	in vitro growth experiments	1647:1673	in vitro growth experiments	1647:1673	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	9	70	theme	pancreatic	1545:1554	arg1	mice					1563:1566	xenograft pancreatic cancer mice	1535:1566	xenograft pancreatic cancer mice subjected to an ERS diet	1535:1591	Consistently, xenograft pancreatic cancer mice subjected to an ERS diet displayed significant retardation in tumor growth.
28346394	6	71	theme	cancer	994:999	arg1	mice					1011:1014	Pancreatic cancer xenograft mice	983:1014	Pancreatic cancer xenograft mice	983:1014	Pancreatic cancer xenograft mice were subjected to an ERS diet to assess tumor volume and weight as compared to mice fed with a control diet.
28346394	12	72	theme	existing	2271:2278	arg1	treatments					2287:2296	existing cancer treatments	2271:2296	existing cancer treatments	2271:2296	These results suggest that engineered dietary interventions could be supportive as a synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients.
28346394	0	73	theme	Cancer	155:160	arg1	Models					162:167	In Vitro and In Vivo Pancreatic Cancer Models	123:167	In Vitro and In Vivo Pancreatic Cancer Models	123:167	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	4	74	theme	cancer	761:766	arg1	model					784:788	an in vivo pancreatic cancer mouse xenograft model	739:788	an in vivo pancreatic cancer mouse xenograft model	739:788	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	11	75	theme	microbiota	2018:2027	arg1	metabolism					1992:2001	metabolism	1992:2001	metabolism	1992:2001	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	11	75	theme	microbiota	2018:2027	arg1	composition					1976:1986	composition	1976:1986	composition	1976:1986	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	9	76	theme	cancer	1556:1561	arg1	mice					1563:1566	xenograft pancreatic cancer mice	1535:1566	xenograft pancreatic cancer mice subjected to an ERS diet	1535:1591	Consistently, xenograft pancreatic cancer mice subjected to an ERS diet displayed significant retardation in tumor growth.
28346394	1	77	theme	fourth	226:231	arg1	cause					241:245	the fourth leading cause	222:245	the fourth leading cause of cancer-related deaths worldwide	222:280	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	1	77	theme	fourth	226:231	arg1	cancer					197:202	BACKGROUND/AIMS Pancreatic cancer	170:202	BACKGROUND/AIMS Pancreatic cancer (PC)	170:207	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	4	78	theme	xenograft	774:782	arg1	model					784:788	an in vivo pancreatic cancer mouse xenograft model	739:788	an in vivo pancreatic cancer mouse xenograft model	739:788	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	10	79	theme	decreased	1829:1837	arg1	formate					1804:1810	formate	1804:1810	formate	1804:1810	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	79	theme	decreased	1829:1837	arg1	lactate					1816:1822	lactate	1816:1822	lactate	1816:1822	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	79	theme	decreased	1829:1837	arg1	propionate					1850:1859	propionate	1850:1859	propionate	1850:1859	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	79	theme	decreased	1829:1837	arg1	amounts					1839:1845	decreased amounts	1829:1845	decreased amounts of propionate	1829:1859	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	80	theme	growth	1656:1661	arg1	experiments					1663:1673	in vitro growth experiments	1647:1673	in vitro growth experiments	1647:1673	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	9	81	theme	ERS	1584:1586	arg1	diet					1588:1591	an ERS diet	1581:1591	an ERS diet	1581:1591	Consistently, xenograft pancreatic cancer mice subjected to an ERS diet displayed significant retardation in tumor growth.
28346394	11	82	theme	mouse	2125:2129	arg1	model					2131:2135	the in vivo PC xenograft mouse model	2100:2135	the in vivo PC xenograft mouse model	2100:2135	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	4	83	theme	resistant-starch	593:608	arg1	effect					569:574	the effect	565:574	the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines	565:664	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	2	84	theme	recent	291:296	arg1	advances					298:305	recent advances	291:305	recent advances in treatment options	291:326	Despite recent advances in treatment options, a modest impact on the outcome of the disease is observed so far.
28346394	11	85	theme	fecal	2012:2016	arg1	microbiota					2018:2027	mouse fecal microbiota	2006:2027	mouse fecal microbiota shown in vitro	2006:2042	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	2	86	from	impact	338:343	arg1	outcome					352:358	the outcome	348:358	the outcome of the disease	348:373	Despite recent advances in treatment options, a modest impact on the outcome of the disease is observed so far.
28346394	0	87	theme	Growth	113:118	arg1	Retardation					92:102	the Retardation	88:102	the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models	88:167	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	10	88	theme	fecal	1680:1684	arg1	cultures					1696:1703	the fecal microbial cultures	1676:1703	the fecal microbial cultures from mice fed with an ERS diet	1676:1734	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	1	89	theme	BACKGROUND/AIMS	170:184	arg1	PC					205:206	PC	205:206	PC	205:206	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	1	89	theme	BACKGROUND/AIMS	170:184	arg1	cancer					197:202	BACKGROUND/AIMS Pancreatic cancer	170:202	BACKGROUND/AIMS Pancreatic cancer (PC)	170:207	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	1	89	theme	BACKGROUND/AIMS	170:184	arg1	cause					241:245	the fourth leading cause	222:245	the fourth leading cause of cancer-related deaths worldwide	222:280	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	0	90	theme	In	123:124	arg1	Models					162:167	In Vitro and In Vivo Pancreatic Cancer Models	123:167	In Vitro and In Vivo Pancreatic Cancer Models	123:167	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	4	91	from	effect	569:574	arg1	growth					638:643	the growth	634:643	the growth of cancer cell lines	634:664	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	9	92	theme	tumor	1630:1634	arg1	growth					1636:1641	tumor growth	1630:1641	tumor growth	1630:1641	Consistently, xenograft pancreatic cancer mice subjected to an ERS diet displayed significant retardation in tumor growth.
28346394	12	93	theme	synergistic	2223:2233	arg1	approach					2235:2242	a synergistic approach	2221:2242	a synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients	2221:2326	These results suggest that engineered dietary interventions could be supportive as a synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients.
28346394	4	94	from	growth	729:734	arg1	model					784:788	an in vivo pancreatic cancer mouse xenograft model	739:788	an in vivo pancreatic cancer mouse xenograft model	739:788	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	8	95	theme	signal-regulated	1380:1395	arg1	kinase					1397:1402	extracellular signal-regulated kinase	1366:1402	extracellular signal-regulated kinase proteins	1366:1411	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	5	96	theme	MIA	821:823	arg1	MATERIALS					791:799	MATERIALS	791:799	MATERIALS	791:799	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	5	96	theme	MIA	821:823	arg1	PaCa-2					825:830	MIA PaCa-2	821:830	MIA PaCa-2	821:830	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	11	97	theme	ERS	1964:1966	arg1	diet					1968:1971	the ERS diet	1960:1971	the ERS diet	1960:1971	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	4	98	theme	cancer	648:653	arg1	lines					660:664	cancer cell lines	648:664	cancer cell lines	648:664	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	5	99	theme	tumor	942:946	arg1	growth					948:953	tumor growth	942:953	tumor growth	942:953	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	10	100	theme	ERS	1727:1729	arg1	diet					1731:1734	an ERS diet	1724:1734	an ERS diet	1724:1734	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	0	101	dep	In	123:124	arg1	Vitro					126:130	Vitro	126:130	Vitro	126:130	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	7	102	theme	isothermal	1221:1230	arg1	microcalorimetry					1232:1247	isothermal microcalorimetry	1221:1247	isothermal microcalorimetry	1221:1247	The composition and activity of fecal microbiota were further analyzed in growth experiments by isothermal microcalorimetry.
28346394	11	103	theme	progression	2085:2095	arg1	decrease					2067:2074	the decrease	2063:2074	the decrease of tumor progression in the in vivo PC xenograft mouse model	2063:2135	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	12	104	theme	engineered	2165:2174	arg1	interventions					2184:2196	engineered dietary interventions	2165:2196	engineered dietary interventions	2165:2196	These results suggest that engineered dietary interventions could be supportive as a synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients.
28346394	8	105	theme	diet-mimicking	1301:1314	arg1	medium					1316:1321	an ERS diet-mimicking medium	1294:1321	an ERS diet-mimicking medium	1294:1321	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	7	106	dep	composition	1129:1139	arg1	The					1125:1127	The	1125:1127	The	1125:1127	The composition and activity of fecal microbiota were further analyzed in growth experiments by isothermal microcalorimetry.
28346394	11	107	theme	xenograft	2115:2123	arg1	model					2131:2135	the in vivo PC xenograft mouse model	2100:2135	the in vivo PC xenograft mouse model	2100:2135	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	10	108	theme	control	1914:1920	arg1	diet					1922:1925	the control diet	1910:1925	the control diet	1910:1925	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	7	109	theme	growth	1199:1204	arg1	experiments					1206:1216	growth experiments	1199:1216	growth experiments	1199:1216	The composition and activity of fecal microbiota were further analyzed in growth experiments by isothermal microcalorimetry.
28346394	3	110	contain	have	458:461	arg1	cycles					451:456	short-term fasting cycles	432:456	short-term fasting cycles	432:456	We have previously demonstrated that short-term fasting cycles have the potential to improve the efficacy of chemotherapy against PC.
28346394	3	110	contain	have	458:461	arg2	potential					467:475	the potential to improve the efficacy of chemotherapy against PC	463:526	the potential to improve the efficacy of chemotherapy against PC	463:526	We have previously demonstrated that short-term fasting cycles have the potential to improve the efficacy of chemotherapy against PC.
28346394	0	111	theme	Resistant-Starch	11:26	arg1	Diet					34:37	Engineered Resistant-Starch (ERS) Diet	0:37	Engineered Resistant-Starch (ERS) Diet	0:37	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	12	112	theme	dietary	2176:2182	arg1	interventions					2184:2196	engineered dietary interventions	2165:2196	engineered dietary interventions	2165:2196	These results suggest that engineered dietary interventions could be supportive as a synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients.
28346394	8	113	theme	levels	1463:1468	arg1	levels					1340:1345	decreased levels	1330:1345	decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels	1330:1468	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	4	114	theme	lines	660:664	arg1	growth					638:643	the growth	634:643	the growth of cancer cell lines	634:664	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	4	115	dep	in	742:743	arg1	vivo					745:748	vivo	745:748	vivo	745:748	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	10	116	theme	residual	1762:1769	arg1	substrates					1771:1780	residual substrates	1762:1780	residual substrates	1762:1780	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	8	117	theme	cancer	1269:1274	arg1	cells					1276:1280	Pancreatic cancer cells	1258:1280	Pancreatic cancer cells cultured in an ERS diet-mimicking medium	1258:1321	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	1	118	theme	deaths	265:270	arg1	worldwide					272:280	cancer-related deaths worldwide	250:280	cancer-related deaths worldwide	250:280	BACKGROUND/AIMS Pancreatic cancer (PC) is ranked as the fourth leading cause of cancer-related deaths worldwide.
28346394	5	119	theme	ERS-mimicking	890:902	arg1	condition					919:927	the ERS-mimicking diet culturing condition	886:927	the ERS-mimicking diet culturing condition	886:927	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	7	120	theme	fecal	1157:1161	arg1	microbiota					1163:1172	fecal microbiota	1157:1172	fecal microbiota	1157:1172	The composition and activity of fecal microbiota were further analyzed in growth experiments by isothermal microcalorimetry.
28346394	0	121	theme	Colon	46:50	arg1	Profile					63:69	Colon Microbiota Profile	46:69	Colon Microbiota Profile	46:69	Engineered Resistant-Starch (ERS) Diet Shapes Colon Microbiota Profile in Parallel with the Retardation of Tumor Growth in In Vitro and In Vivo Pancreatic Cancer Models.
28346394	5	122	theme	culturing	909:917	arg1	condition					919:927	the ERS-mimicking diet culturing condition	886:927	the ERS-mimicking diet culturing condition	886:927	MATERIALS AND METHODS BxPC-3, MIA PaCa-2 and PANC-1 cells were cultured in the control, and in the ERS-mimicking diet culturing condition, to evaluate tumor growth and proliferation pathways.
28346394	8	123	theme	rapamycin	1452:1460	arg1	target					1442:1447	mammalian target	1432:1447	mammalian target of rapamycin	1432:1460	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	11	124	theme	positive	1941:1948	arg1	effect					1950:1955	A positive effect	1939:1955	A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro	1939:2042	CONCLUSION A positive effect of the ERS diet on composition and metabolism of mouse fecal microbiota shown in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model.
28346394	12	125	theme	cancer	2312:2317	arg1	patients					2319:2326	pancreatic cancer patients	2301:2326	pancreatic cancer patients	2301:2326	These results suggest that engineered dietary interventions could be supportive as a synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients.
28346394	2	126	theme	treatment	310:318	arg1	options					320:326	treatment options	310:326	treatment options	310:326	Despite recent advances in treatment options, a modest impact on the outcome of the disease is observed so far.
28346394	8	127	theme	standard	1504:1511	arg1	medium					1513:1518	standard medium	1504:1518	standard medium	1504:1518	RESULTS Pancreatic cancer cells cultured in an ERS diet-mimicking medium showed decreased levels of phospho-ERK1/2 (extracellular signal-regulated kinase proteins) and phospho-mTOR (mammalian target of rapamycin) levels, as compared to those cultured in standard medium.
28346394	4	128	theme	fecal	698:702	arg1	microbiota					704:713	fecal microbiota	698:713	fecal microbiota	698:713	The aim of this study was to assess the effect of an engineered resistant-starch (ERS) mimicking diet on the growth of cancer cell lines in vitro, on the composition of fecal microbiota, and on tumor growth in an in vivo pancreatic cancer mouse xenograft model.
28346394	3	129	theme	fasting	443:449	arg1	cycles					451:456	short-term fasting cycles	432:456	short-term fasting cycles	432:456	We have previously demonstrated that short-term fasting cycles have the potential to improve the efficacy of chemotherapy against PC.
28346394	10	130	from	growth	1752:1757	arg1	substrates					1771:1780	residual substrates	1762:1780	residual substrates	1762:1780	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	9	131	theme	xenograft	1535:1543	arg1	mice					1563:1566	xenograft pancreatic cancer mice	1535:1566	xenograft pancreatic cancer mice subjected to an ERS diet	1535:1591	Consistently, xenograft pancreatic cancer mice subjected to an ERS diet displayed significant retardation in tumor growth.
28346394	6	132	theme	xenograft	1001:1009	arg1	mice					1011:1014	Pancreatic cancer xenograft mice	983:1014	Pancreatic cancer xenograft mice	983:1014	Pancreatic cancer xenograft mice were subjected to an ERS diet to assess tumor volume and weight as compared to mice fed with a control diet.
28346394	12	133	theme	cancer	2280:2285	arg1	treatments					2287:2296	existing cancer treatments	2271:2296	existing cancer treatments	2271:2296	These results suggest that engineered dietary interventions could be supportive as a synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients.
28346394	10	134	theme	lactate	1816:1822	arg1	growth					1752:1757	enhanced growth	1743:1757	enhanced growth on residual substrates	1743:1780	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	134	theme	lactate	1816:1822	arg1	amounts					1839:1845	decreased amounts	1829:1845	decreased amounts of propionate	1829:1859	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	134	theme	lactate	1816:1822	arg1	formate					1804:1810	formate	1804:1810	formate	1804:1810	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	134	theme	lactate	1816:1822	arg1	lactate					1816:1822	lactate	1816:1822	lactate	1816:1822	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	134	theme	lactate	1816:1822	arg1	propionate					1850:1859	propionate	1850:1859	propionate	1850:1859	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	10	134	theme	lactate	1816:1822	arg1	production					1790:1799	higher production	1783:1799	higher production of formate and lactate	1783:1822	In in vitro growth experiments, the fecal microbial cultures from mice fed with an ERS diet showed enhanced growth on residual substrates, higher production of formate and lactate, and decreased amounts of propionate, compared to fecal microbiota from mice fed with the control diet.
28346394	2	135	theme	modest	331:336	arg1	impact					338:343	a modest impact	329:343	a modest impact on the outcome of the disease	329:373	Despite recent advances in treatment options, a modest impact on the outcome of the disease is observed so far.
26572342	0	0	theme	polyethylene	72:83	arg1	starch					108:113	linear low density polyethylene and Vietnamese cassava starch	53:113	linear low density polyethylene and Vietnamese cassava starch	53:113	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch biobased blend.
26572342	7	1	theme	film	1021:1024	arg1	weight					1001:1006	weight	1001:1006	weight of LLDPE/TPS film	1001:1024	The biodegradation test shows that more than 60% in weight of LLDPE/TPS film is degraded into CO2, H2O, methane and biomass after 5 months in compost soil.
26572342	1	2	theme	polyethylene	150:161	arg1	TPS					195:197	TPS	195:197	TPS	195:197	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	1	2	theme	polyethylene	150:161	arg1	starch					187:192	low density polyethylene (LLDPE)/thermal plastic starch	138:192	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend	131:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	2	3	from	°C	437:438	arg1	method					423:428	melt mixing method	411:428	melt mixing method at 160 °C for 7 min	411:448	The LLDPE/TPS blend and its nanocomposites were elaborated by melt mixing method at 160 °C for 7 min.
26572342	6	4	theme	TPS	917:919	arg1	phase					921:925	TPS phase	917:925	TPS phase	917:925	The effect of N15 on morphology and particles size of TPS phase is also investigated.
26572342	0	5	theme	density	64:70	arg1	polyethylene					72:83	low density polyethylene	60:83	low density polyethylene	60:83	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch biobased blend.
26572342	7	6	theme	LLDPE/TPS	1011:1019	arg1	film					1021:1024	LLDPE/TPS film	1011:1024	LLDPE/TPS film	1011:1024	The biodegradation test shows that more than 60% in weight of LLDPE/TPS film is degraded into CO2, H2O, methane and biomass after 5 months in compost soil.
26572342	0	7	theme	Vietnamese	89:98	arg1	starch					108:113	linear low density polyethylene and Vietnamese cassava starch	53:113	linear low density polyethylene and Vietnamese cassava starch	53:113	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch biobased blend.
26572342	0	8	from	Effect	0:5	arg1	properties					39:48	properties	39:48	properties	39:48	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch biobased blend.
26572342	0	8	from	Effect	0:5	arg1	morphology					24:33	morphology	24:33	morphology	24:33	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch biobased blend.
26572342	4	9	theme	transmission	677:688	arg1	TEM					713:715	TEM	713:715	TEM	713:715	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	4	9	theme	transmission	677:688	arg1	microscopy					701:710	transmission electronic microscopy	677:710	transmission electronic microscopy (TEM)	677:716	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	1	10	theme	biobased	233:240	arg1	material					256:263	the biobased nanocomposite material	229:263	the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase	229:346	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	1	11	theme	reinforced	331:340	arg1	phase					342:346	the reinforced phase	327:346	the reinforced phase	327:346	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	1	11	theme	reinforced	331:340	arg1	nanofil15					282:290	organoclay nanofil15	271:290	organoclay nanofil15 (N15) modified by alkilammonium	271:322	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	6	12	theme	particles	899:907	arg1	size					909:912	morphology and particles size	884:912	morphology and particles size of TPS phase	884:925	The effect of N15 on morphology and particles size of TPS phase is also investigated.
26572342	3	13	theme	compounded	459:468	arg1	sample					470:475	the compounded sample	455:475	the compounded sample	455:475	And the compounded sample was filmed by blowing method at three different zones of temperature profile which are 160-170-165 °C.
26572342	1	14	theme	nanocomposite	242:254	arg1	material					256:263	the biobased nanocomposite material	229:263	the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase	229:346	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	0	15	theme	starch	108:113	arg1	properties					39:48	properties	39:48	properties	39:48	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch biobased blend.
26572342	0	15	theme	starch	108:113	arg1	morphology					24:33	morphology	24:33	morphology	24:33	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch biobased blend.
26572342	5	16	theme	thermal	769:775	arg1	properties					792:801	The thermal and mechanical properties	765:801	The thermal and mechanical properties of materials	765:814	The thermal and mechanical properties of materials are enhanced when N15 is added to the mixture.
26572342	4	17	theme	blend	623:627	arg1	matrix					629:634	the polymer blend matrix	611:634	the polymer blend matrix	611:634	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	1	18	theme	LLDPE	164:168	arg1	TPS					195:197	TPS	195:197	TPS	195:197	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	1	18	theme	LLDPE	164:168	arg1	starch					187:192	low density polyethylene (LLDPE)/thermal plastic starch	138:192	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend	131:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	0	19	theme	cassava	100:106	arg1	starch					108:113	linear low density polyethylene and Vietnamese cassava starch	53:113	linear low density polyethylene and Vietnamese cassava starch	53:113	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch biobased blend.
26572342	7	20	theme	compost	1091:1097	arg1	soil					1099:1102	compost soil	1091:1102	compost soil	1091:1102	The biodegradation test shows that more than 60% in weight of LLDPE/TPS film is degraded into CO2, H2O, methane and biomass after 5 months in compost soil.
26572342	1	21	theme	/thermal	170:177	arg1	TPS					195:197	TPS	195:197	TPS	195:197	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	1	21	theme	/thermal	170:177	arg1	starch					187:192	low density polyethylene (LLDPE)/thermal plastic starch	138:192	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend	131:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	1	22	theme	plastic	179:185	arg1	TPS					195:197	TPS	195:197	TPS	195:197	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	1	22	theme	plastic	179:185	arg1	starch					187:192	low density polyethylene (LLDPE)/thermal plastic starch	138:192	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend	131:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	2	23	theme	mixing	416:421	arg1	method					423:428	melt mixing method	411:428	melt mixing method at 160 °C for 7 min	411:448	The LLDPE/TPS blend and its nanocomposites were elaborated by melt mixing method at 160 °C for 7 min.
26572342	4	24	theme	semi-exfoliated	725:739	arg1	structure					741:749	a semi-exfoliated structure	723:749	a semi-exfoliated structure	723:749	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	4	25	theme	electronic	690:699	arg1	TEM					713:715	TEM	713:715	TEM	713:715	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	4	25	theme	electronic	690:699	arg1	microscopy					701:710	transmission electronic microscopy	677:710	transmission electronic microscopy (TEM)	677:716	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	1	26	theme	starch	187:192	arg1	blend					200:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend	131:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend	131:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	2	27	theme	melt	411:414	arg1	method					423:428	melt mixing method	411:428	melt mixing method at 160 °C for 7 min	411:448	The LLDPE/TPS blend and its nanocomposites were elaborated by melt mixing method at 160 °C for 7 min.
26572342	1	28	theme	organoclay	271:280	arg1	phase					342:346	the reinforced phase	327:346	the reinforced phase	327:346	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	1	28	theme	organoclay	271:280	arg1	nanofil15					282:290	organoclay nanofil15	271:290	organoclay nanofil15 (N15) modified by alkilammonium	271:322	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	1	28	theme	organoclay	271:280	arg1	N15					293:295	N15	293:295	N15	293:295	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	3	29	theme	different	515:523	arg1	zones					525:529	three different zones	509:529	three different zones of temperature profile which are 160-170-165 °C	509:577	And the compounded sample was filmed by blowing method at three different zones of temperature profile which are 160-170-165 °C.
26572342	4	30	from	dispersion	589:598	arg1	matrix					629:634	the polymer blend matrix	611:634	the polymer blend matrix	611:634	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	4	31	theme	good	584:587	arg1	dispersion					589:598	The good dispersion	580:598	The good dispersion of clay in the polymer blend matrix	580:634	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	6	32	theme	N15	877:879	arg1	effect					867:872	The effect	863:872	The effect of N15 on morphology and particles size of TPS phase	863:925	The effect of N15 on morphology and particles size of TPS phase is also investigated.
26572342	7	33	from	months	1081:1086	arg1	soil					1099:1102	compost soil	1091:1102	compost soil	1091:1102	The biodegradation test shows that more than 60% in weight of LLDPE/TPS film is degraded into CO2, H2O, methane and biomass after 5 months in compost soil.
26572342	4	34	theme	polymer	615:621	arg1	matrix					629:634	the polymer blend matrix	611:634	the polymer blend matrix	611:634	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	4	35	theme	clay	603:606	arg1	dispersion					589:598	The good dispersion	580:598	The good dispersion of clay in the polymer blend matrix	580:634	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	5	36	theme	materials	806:814	arg1	properties					792:801	The thermal and mechanical properties	765:801	The thermal and mechanical properties of materials	765:814	The thermal and mechanical properties of materials are enhanced when N15 is added to the mixture.
26572342	5	37	theme	mechanical	781:790	arg1	properties					792:801	The thermal and mechanical properties	765:801	The thermal and mechanical properties of materials	765:814	The thermal and mechanical properties of materials are enhanced when N15 is added to the mixture.
26572342	7	38	theme	biodegradation	953:966	arg1	test					968:971	The biodegradation test	949:971	The biodegradation test	949:971	The biodegradation test shows that more than 60% in weight of LLDPE/TPS film is degraded into CO2, H2O, methane and biomass after 5 months in compost soil.
26572342	6	39	theme	morphology	884:893	arg1	size					909:912	morphology and particles size	884:912	morphology and particles size of TPS phase	884:925	The effect of N15 on morphology and particles size of TPS phase is also investigated.
26572342	3	40	theme	temperature	534:544	arg1	°C					576:577	160-170-165 °C	564:577	160-170-165 °C	564:577	And the compounded sample was filmed by blowing method at three different zones of temperature profile which are 160-170-165 °C.
26572342	3	40	theme	temperature	534:544	arg1	profile					546:552	temperature profile	534:552	temperature profile which are 160-170-165 °C	534:577	And the compounded sample was filmed by blowing method at three different zones of temperature profile which are 160-170-165 °C.
26572342	3	41	theme	profile	546:552	arg1	zones					525:529	three different zones	509:529	three different zones of temperature profile which are 160-170-165 °C	509:577	And the compounded sample was filmed by blowing method at three different zones of temperature profile which are 160-170-165 °C.
26572342	4	42	theme	X-ray	649:653	arg1	diffraction					655:665	X-ray diffraction	649:665	X-ray diffraction (XRD)	649:671	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	4	42	theme	X-ray	649:653	arg1	XRD					668:670	XRD	668:670	XRD	668:670	The good dispersion of clay in the polymer blend matrix is showed by X-ray diffraction (XRD) and transmission electronic microscopy (TEM), and a semi-exfoliated structure was obtained.
26572342	6	43	from	effect	867:872	arg1	size					909:912	morphology and particles size	884:912	morphology and particles size of TPS phase	884:925	The effect of N15 on morphology and particles size of TPS phase is also investigated.
26572342	2	44	dep	LLDPE/TPS	353:361	arg1	blend					363:367	blend	363:367	blend	363:367	The LLDPE/TPS blend and its nanocomposites were elaborated by melt mixing method at 160 °C for 7 min.
26572342	2	44	dep	LLDPE/TPS	353:361	arg1	nanocomposites					377:390	its nanocomposites	373:390	its nanocomposites	373:390	The LLDPE/TPS blend and its nanocomposites were elaborated by melt mixing method at 160 °C for 7 min.
26572342	7	45	from	%	996:996	arg1	weight					1001:1006	weight	1001:1006	weight of LLDPE/TPS film	1001:1024	The biodegradation test shows that more than 60% in weight of LLDPE/TPS film is degraded into CO2, H2O, methane and biomass after 5 months in compost soil.
26572342	1	46	theme	Linear	131:136	arg1	blend					200:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend	131:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend	131:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	0	47	theme	low	60:62	arg1	polyethylene					72:83	low density polyethylene	60:83	low density polyethylene	60:83	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch biobased blend.
26572342	3	48	theme	160-170-165	564:574	arg1	°C					576:577	160-170-165 °C	564:577	160-170-165 °C	564:577	And the compounded sample was filmed by blowing method at three different zones of temperature profile which are 160-170-165 °C.
26572342	3	48	theme	160-170-165	564:574	arg1	profile					546:552	temperature profile	534:552	temperature profile which are 160-170-165 °C	534:577	And the compounded sample was filmed by blowing method at three different zones of temperature profile which are 160-170-165 °C.
26572342	1	49	theme	low	138:140	arg1	TPS					195:197	TPS	195:197	TPS	195:197	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	1	49	theme	low	138:140	arg1	starch					187:192	low density polyethylene (LLDPE)/thermal plastic starch	138:192	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend	131:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	0	50	theme	linear	53:58	arg1	starch					108:113	linear low density polyethylene and Vietnamese cassava starch	53:113	linear low density polyethylene and Vietnamese cassava starch	53:113	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch biobased blend.
26572342	0	51	theme	organoclay	10:19	arg1	Effect					0:5	Effect	0:5	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch	0:113	Effect of organoclay on morphology and properties of linear low density polyethylene and Vietnamese cassava starch biobased blend.
26572342	1	52	theme	density	142:148	arg1	TPS					195:197	TPS	195:197	TPS	195:197	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	1	52	theme	density	142:148	arg1	starch					187:192	low density polyethylene (LLDPE)/thermal plastic starch	138:192	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend	131:204	Linear low density polyethylene (LLDPE)/thermal plastic starch (TPS) blend was studied to prepare the biobased nanocomposite material using organoclay nanofil15 (N15) modified by alkilammonium as the reinforced phase.
26572342	6	53	theme	phase	921:925	arg1	size					909:912	morphology and particles size	884:912	morphology and particles size of TPS phase	884:925	The effect of N15 on morphology and particles size of TPS phase is also investigated.
27316766	6	0	theme	immobilized	814:824	arg1	β-gal					826:830	6.285±0.22U/g gel immobilized β-gal	796:830	6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value	796:884	The verification experiment run at these optimum conditions offered 6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value.
27316766	5	1	theme	PEI	573:575	arg1	solution					577:584	a 3.49% PEI solution	565:584	a 3.49% PEI solution of pH 10.55	565:596	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	6	2	theme	gel	810:812	arg1	β-gal					826:830	6.285±0.22U/g gel immobilized β-gal	796:830	6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value	796:884	The verification experiment run at these optimum conditions offered 6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value.
27316766	6	3	theme	verification	732:743	arg1	run					756:758	The verification experiment run	728:758	The verification experiment run at these optimum conditions	728:786	The verification experiment run at these optimum conditions offered 6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value.
27316766	6	4	theme	experiment	745:754	arg1	run					756:758	The verification experiment run	728:758	The verification experiment run at these optimum conditions	728:786	The verification experiment run at these optimum conditions offered 6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value.
27316766	4	5	theme	observed	457:464	arg1	response					505:512	a response	503:512	a response	503:512	The central composite design (CCD) was applied to optimize the PEI/GA treatment while employing the observed activity of the immobilized β-gal as a response.
27316766	4	5	theme	observed	457:464	arg1	activity					466:473	the observed activity	453:473	the observed activity of the immobilized β-gal	453:498	The central composite design (CCD) was applied to optimize the PEI/GA treatment while employing the observed activity of the immobilized β-gal as a response.
27316766	7	6	theme	initial	994:1000	arg1	activity					1002:1009	its initial activity	990:1009	its initial activity	990:1009	The reusability study revealed that the β-gal immobilized onto the treated CP beads retained 79.34% of its initial activity after being used for fourteen times.
27316766	5	7	theme	CP	551:552	arg1	beads					554:558	the CP beads	547:558	the CP beads	547:558	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	4	8	theme	PEI/GA	420:425	arg1	treatment					427:435	the PEI/GA treatment	416:435	the PEI/GA treatment	416:435	The central composite design (CCD) was applied to optimize the PEI/GA treatment while employing the observed activity of the immobilized β-gal as a response.
27316766	5	9	theme	pH	589:590	arg1	solution					577:584	a 3.49% PEI solution	565:584	a 3.49% PEI solution of pH 10.55	565:596	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	4	10	theme	composite	369:377	arg1	CCD					387:389	CCD	387:389	CCD	387:389	The central composite design (CCD) was applied to optimize the PEI/GA treatment while employing the observed activity of the immobilized β-gal as a response.
27316766	4	10	theme	composite	369:377	arg1	design					379:384	The central composite design	357:384	The central composite design (CCD)	357:390	The central composite design (CCD) was applied to optimize the PEI/GA treatment while employing the observed activity of the immobilized β-gal as a response.
27316766	6	11	with	agreement	851:859	arg1	value					880:884	the predicted value	866:884	the predicted value	866:884	The verification experiment run at these optimum conditions offered 6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value.
27316766	5	12	theme	0.1M	634:637	arg1	buffer					649:654	0.1M phosphate buffer	634:654	0.1M phosphate buffer pH 7.02	634:662	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	4	13	theme	central	361:367	arg1	CCD					387:389	CCD	387:389	CCD	387:389	The central composite design (CCD) was applied to optimize the PEI/GA treatment while employing the observed activity of the immobilized β-gal as a response.
27316766	4	13	theme	central	361:367	arg1	design					379:384	The central composite design	357:384	The central composite design (CCD)	357:390	The central composite design (CCD) was applied to optimize the PEI/GA treatment while employing the observed activity of the immobilized β-gal as a response.
27316766	5	14	theme	phosphate	639:647	arg1	buffer					649:654	0.1M phosphate buffer	634:654	0.1M phosphate buffer pH 7.02	634:662	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	6	15	theme	optimum	769:775	arg1	conditions					777:786	these optimum conditions	763:786	these optimum conditions	763:786	The verification experiment run at these optimum conditions offered 6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value.
27316766	5	16	theme	6.25U/g	702:708	arg1	gel					710:712	6.25U/g gel	702:712	6.25U/g gel	702:712	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	5	17	theme	3.49	567:570	arg1	%					571:571	%	571:571	%	571:571	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	1	18	theme	calcium	90:96	arg1	pectinate					98:106	calcium pectinate	90:106	The calcium pectinate (CP) gel beads	86:121	The calcium pectinate (CP) gel beads were treated with polyethyleneimine (PEI) and glutaraldehyde (GA).
27316766	1	18	theme	calcium	90:96	arg1	CP					109:110	CP	109:110	CP	109:110	The calcium pectinate (CP) gel beads were treated with polyethyleneimine (PEI) and glutaraldehyde (GA).
27316766	0	19	theme	Treated	0:6	arg1	calcium					8:14	Treated calcium	0:14	Treated calcium	0:14	Treated calcium pectinate beads for the covalent immobilization of β-d-galactosidase.
27316766	6	20	theme	predicted	870:878	arg1	value					880:884	the predicted value	866:884	the predicted value	866:884	The verification experiment run at these optimum conditions offered 6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value.
27316766	5	21	theme	%	571:571	arg1	solution					577:584	a 3.49% PEI solution	565:584	a 3.49% PEI solution of pH 10.55	565:596	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	1	22	theme	pectinate	98:106	arg1	beads					117:121	The calcium pectinate (CP) gel beads	86:121	The calcium pectinate (CP) gel beads	86:121	The calcium pectinate (CP) gel beads were treated with polyethyleneimine (PEI) and glutaraldehyde (GA).
27316766	5	23	theme	gel	710:712	arg1	immobilization					684:697	the immobilization	680:697	the immobilization of 6.25U/g gel of the β-gal	680:725	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	7	24	theme	treated	954:960	arg1	beads					965:969	the treated CP beads	950:969	the treated CP beads	950:969	The reusability study revealed that the β-gal immobilized onto the treated CP beads retained 79.34% of its initial activity after being used for fourteen times.
27316766	5	25	theme	5.66	613:616	arg1	%					617:617	%	617:617	%	617:617	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	5	26	theme	β-gal	721:725	arg1	immobilization					684:697	the immobilization	680:697	the immobilization of 6.25U/g gel of the β-gal	680:725	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	5	27	theme	%	617:617	arg1	solution					622:629	a 5.66% GA solution	611:629	a 5.66% GA solution in 0.1M phosphate buffer pH 7.02	611:662	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	6	28	theme	close	845:849	arg1	agreement					851:859	close agreement	845:859	close agreement with the predicted value	845:884	The verification experiment run at these optimum conditions offered 6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value.
27316766	5	29	theme	GA	619:620	arg1	solution					622:629	a 5.66% GA solution	611:629	a 5.66% GA solution in 0.1M phosphate buffer pH 7.02	611:662	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	5	30	from	solution	622:629	arg1	buffer					649:654	0.1M phosphate buffer	634:654	0.1M phosphate buffer pH 7.02	634:662	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	6	31	from	conditions	777:786	arg1	run					756:758	The verification experiment run	728:758	The verification experiment run at these optimum conditions	728:786	The verification experiment run at these optimum conditions offered 6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value.
27316766	5	32	dep	buffer	649:654	arg1	pH					656:657	pH 7.02	656:662	0.1M phosphate buffer pH 7.02	634:662	The CCD predicted that treating the CP beads with a 3.49% PEI solution of pH 10.55, followed by a 5.66% GA solution in 0.1M phosphate buffer pH 7.02 would allow for the immobilization of 6.25U/g gel of the β-gal.
27316766	7	33	theme	CP	962:963	arg1	beads					965:969	the treated CP beads	950:969	the treated CP beads	950:969	The reusability study revealed that the β-gal immobilized onto the treated CP beads retained 79.34% of its initial activity after being used for fourteen times.
27316766	0	34	theme	covalent	40:47	arg1	immobilization					49:62	the covalent immobilization	36:62	the covalent immobilization of β-d-galactosidase	36:83	Treated calcium pectinate beads for the covalent immobilization of β-d-galactosidase.
27316766	4	35	theme	β-gal	494:498	arg1	response					505:512	a response	503:512	a response	503:512	The central composite design (CCD) was applied to optimize the PEI/GA treatment while employing the observed activity of the immobilized β-gal as a response.
27316766	4	35	theme	β-gal	494:498	arg1	activity					466:473	the observed activity	453:473	the observed activity of the immobilized β-gal	453:498	The central composite design (CCD) was applied to optimize the PEI/GA treatment while employing the observed activity of the immobilized β-gal as a response.
27316766	3	36	theme	CP	279:280	arg1	beads					282:286	the CP beads	275:286	the CP beads	275:286	Moreover, it enabled the CP beads to covalently immobilize enzymes, such as β-d-galactosidase (β-gal).
27316766	1	37	theme	gel	113:115	arg1	beads					117:121	The calcium pectinate (CP) gel beads	86:121	The calcium pectinate (CP) gel beads	86:121	The calcium pectinate (CP) gel beads were treated with polyethyleneimine (PEI) and glutaraldehyde (GA).
27316766	4	38	theme	immobilized	482:492	arg1	β-gal					494:498	the immobilized β-gal	478:498	the immobilized β-gal	478:498	The central composite design (CCD) was applied to optimize the PEI/GA treatment while employing the observed activity of the immobilized β-gal as a response.
27316766	2	39	theme	mechanical	233:242	arg1	strength					244:251	the beads' mechanical strength	222:251	the beads' mechanical strength	222:251	This treatment greatly enhanced the beads' mechanical strength.
27316766	0	40	theme	β-d-galactosidase	67:83	arg1	immobilization					49:62	the covalent immobilization	36:62	the covalent immobilization of β-d-galactosidase	36:83	Treated calcium pectinate beads for the covalent immobilization of β-d-galactosidase.
27316766	7	41	theme	reusability	891:901	arg1	study					903:907	The reusability study	887:907	The reusability study	887:907	The reusability study revealed that the β-gal immobilized onto the treated CP beads retained 79.34% of its initial activity after being used for fourteen times.
27316766	6	42	theme	6.285±0.22U/g	796:808	arg1	β-gal					826:830	6.285±0.22U/g gel immobilized β-gal	796:830	6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value	796:884	The verification experiment run at these optimum conditions offered 6.285±0.22U/g gel immobilized β-gal which was in close agreement with the predicted value.
27316766	7	43	theme	activity	1002:1009	arg1	activity					1002:1009	its initial activity	990:1009	its initial activity	990:1009	The reusability study revealed that the β-gal immobilized onto the treated CP beads retained 79.34% of its initial activity after being used for fourteen times.
27316766	7	43	theme	activity	1002:1009	arg1	%					985:985	79.34%	980:985	79.34% of its initial activity	980:1009	The reusability study revealed that the β-gal immobilized onto the treated CP beads retained 79.34% of its initial activity after being used for fourteen times.
25952848	6	0	theme	microscopy	969:978	arg1	results					903:909	The results	899:909	The results of scanning electron microscopy and transmission electron microscopy	899:978	The results of scanning electron microscopy and transmission electron microscopy showed that the sugarcane bagasse extract might change cell morphology and internal structure.
25952848	2	1	theme	470	350:352	arg1	mg					354:355	mg	354:355	mg	354:355	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	5	2	theme	sodium	723:728	arg1	electrophoresis					765:779	sodium dodecyl sulfate polyacrylamide gel electrophoresis	723:779	sodium dodecyl sulfate polyacrylamide gel electrophoresis	723:779	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	5	3	theme	damaged	843:849	arg1	proteins					860:867	the damaged cellular proteins	839:867	the damaged cellular proteins	839:867	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	6	4	theme	bagasse	1006:1012	arg1	extract					1014:1020	the sugarcane bagasse extract	992:1020	the sugarcane bagasse extract	992:1020	The results of scanning electron microscopy and transmission electron microscopy showed that the sugarcane bagasse extract might change cell morphology and internal structure.
25952848	5	5	theme	antibacterial	795:807	arg1	mechanism					809:817	the antibacterial mechanism	791:817	the antibacterial mechanism	791:817	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	6	6	theme	sugarcane	996:1004	arg1	extract					1014:1020	the sugarcane bagasse extract	992:1020	the sugarcane bagasse extract	992:1020	The results of scanning electron microscopy and transmission electron microscopy showed that the sugarcane bagasse extract might change cell morphology and internal structure.
25952848	2	7	dep	bagasse	336:342	arg1	mg/g					327:330	mg/g	327:330	mg/g	327:330	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	3	8	theme	coli	523:526	arg1	growth					454:459	the growth	450:459	the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium	450:554	The sugarcane bagasse extract showed bacteriostatic activity against the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium.
25952848	3	9	theme	monocytogenes	496:508	arg1	growth					454:459	the growth	450:459	the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium	450:554	The sugarcane bagasse extract showed bacteriostatic activity against the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium.
25952848	0	10	theme	food-borne	103:112	arg1	pathogens					114:122	food-borne pathogens	103:122	food-borne pathogens	103:122	The antibiotic activity and mechanisms of sugarcane (Saccharum officinarum L.) bagasse extract against food-borne pathogens.
25952848	5	11	theme	bagasse	882:888	arg1	extract					890:896	sugarcane bagasse extract	872:896	sugarcane bagasse extract	872:896	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	4	12	theme	electric	618:625	arg1	conductivity					627:638	the electric conductivity	614:638	the electric conductivity of bacterial cell suspensions causing cellular leaking of electrolytes	614:709	Additionally, the sugarcane bagasse extract can increase the electric conductivity of bacterial cell suspensions causing cellular leaking of electrolytes.
25952848	2	13	theme	sugarcane	279:287	arg1	bagasse					289:295	sugarcane bagasse	279:295	sugarcane bagasse	279:295	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	4	14	theme	electrolytes	698:709	arg1	leaking					687:693	leaking	687:693	leaking	687:693	Additionally, the sugarcane bagasse extract can increase the electric conductivity of bacterial cell suspensions causing cellular leaking of electrolytes.
25952848	0	15	theme	antibiotic	4:13	arg1	activity					15:22	The antibiotic activity	0:22	The antibiotic activity	0:22	The antibiotic activity and mechanisms of sugarcane (Saccharum officinarum L.) bagasse extract against food-borne pathogens.
25952848	1	16	contain	contains	143:150	arg2	compositions					160:171	natural compositions	152:171	natural compositions that can significantly inhibit food-borne pathogens growth	152:230	Sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth.
25952848	1	16	contain	contains	143:150	arg1	bagasse					135:141	Sugarcane bagasse	125:141	Sugarcane bagasse	125:141	Sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth.
25952848	5	17	theme	dodecyl	730:736	arg1	electrophoresis					765:779	sodium dodecyl sulfate polyacrylamide gel electrophoresis	723:779	sodium dodecyl sulfate polyacrylamide gel electrophoresis	723:779	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	3	18	theme	sugarcane	385:393	arg1	extract					403:409	The sugarcane bagasse extract	381:409	The sugarcane bagasse extract	381:409	The sugarcane bagasse extract showed bacteriostatic activity against the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium.
25952848	4	19	theme	bagasse	585:591	arg1	extract					593:599	the sugarcane bagasse extract	571:599	the sugarcane bagasse extract	571:599	Additionally, the sugarcane bagasse extract can increase the electric conductivity of bacterial cell suspensions causing cellular leaking of electrolytes.
25952848	2	20	dep	mg/g	327:330	arg1	4					325:325	4	325:325	4	325:325	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	1	21	theme	food-borne	204:213	arg1	pathogens					215:223	food-borne pathogens	204:223	food-borne pathogens growth	204:230	Sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth.
25952848	4	22	theme	sugarcane	575:583	arg1	extract					593:599	the sugarcane bagasse extract	571:599	the sugarcane bagasse extract	571:599	Additionally, the sugarcane bagasse extract can increase the electric conductivity of bacterial cell suspensions causing cellular leaking of electrolytes.
25952848	5	23	theme	gel	761:763	arg1	electrophoresis					765:779	sodium dodecyl sulfate polyacrylamide gel electrophoresis	723:779	sodium dodecyl sulfate polyacrylamide gel electrophoresis	723:779	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	1	24	theme	pathogens	215:223	arg1	growth					225:230	food-borne pathogens growth	204:230	food-borne pathogens growth	204:230	Sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth.
25952848	3	25	theme	typhimurium	544:554	arg1	growth					454:459	the growth	450:459	the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium	450:554	The sugarcane bagasse extract showed bacteriostatic activity against the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium.
25952848	5	26	theme	electrophoresis	765:779	arg1	Results					712:718	Results	712:718	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis	712:779	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	6	27	theme	electron	960:967	arg1	microscopy					969:978	transmission electron microscopy	947:978	transmission electron microscopy	947:978	The results of scanning electron microscopy and transmission electron microscopy showed that the sugarcane bagasse extract might change cell morphology and internal structure.
25952848	0	28	dep	Saccharum	53:61	arg1	L.					75:76	Saccharum officinarum L.	53:76	Saccharum officinarum L.	53:76	The antibiotic activity and mechanisms of sugarcane (Saccharum officinarum L.) bagasse extract against food-borne pathogens.
25952848	5	29	theme	cellular	851:858	arg1	proteins					860:867	the damaged cellular proteins	839:867	the damaged cellular proteins	839:867	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	6	30	theme	internal	1055:1062	arg1	structure					1064:1072	internal structure	1055:1072	internal structure	1055:1072	The results of scanning electron microscopy and transmission electron microscopy showed that the sugarcane bagasse extract might change cell morphology and internal structure.
25952848	6	31	theme	transmission	947:958	arg1	microscopy					969:978	transmission electron microscopy	947:978	transmission electron microscopy	947:978	The results of scanning electron microscopy and transmission electron microscopy showed that the sugarcane bagasse extract might change cell morphology and internal structure.
25952848	2	32	from	content	268:274	arg1	bagasse					289:295	sugarcane bagasse	279:295	sugarcane bagasse	279:295	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	3	33	theme	bacteriostatic	418:431	arg1	activity					433:440	bacteriostatic activity	418:440	bacteriostatic activity against the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium	418:554	The sugarcane bagasse extract showed bacteriostatic activity against the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium.
25952848	3	34	theme	bagasse	395:401	arg1	extract					403:409	The sugarcane bagasse extract	381:409	The sugarcane bagasse extract	381:409	The sugarcane bagasse extract showed bacteriostatic activity against the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium.
25952848	2	35	located	detected	301:308	arg1	study					248:252	the present study	236:252	the present study	236:252	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	2	35	located	detected	301:308	arg2	content					268:274	the phenolic content	255:274	the phenolic content in sugarcane bagasse	255:295	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	2	35	located	detected	301:308	arg2	bagasse					336:342	higher than 4 mg/g dry bagasse	313:342	higher than 4 mg/g dry bagasse	313:342	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	5	36	theme	sugarcane	872:880	arg1	extract					890:896	sugarcane bagasse extract	872:896	sugarcane bagasse extract	872:896	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	6	37	theme	microscopy	932:941	arg1	results					903:909	The results	899:909	The results of scanning electron microscopy and transmission electron microscopy	899:978	The results of scanning electron microscopy and transmission electron microscopy showed that the sugarcane bagasse extract might change cell morphology and internal structure.
25952848	2	38	theme	present	240:246	arg1	study					248:252	the present study	236:252	the present study	236:252	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	0	39	theme	sugarcane	42:50	arg1	activity					15:22	The antibiotic activity	0:22	The antibiotic activity	0:22	The antibiotic activity and mechanisms of sugarcane (Saccharum officinarum L.) bagasse extract against food-borne pathogens.
25952848	0	39	theme	sugarcane	42:50	arg1	mechanisms					28:37	mechanisms	28:37	mechanisms of sugarcane (Saccharum officinarum L.)	28:77	The antibiotic activity and mechanisms of sugarcane (Saccharum officinarum L.) bagasse extract against food-borne pathogens.
25952848	6	40	theme	cell	1035:1038	arg1	morphology					1040:1049	cell morphology	1035:1049	cell morphology	1035:1049	The results of scanning electron microscopy and transmission electron microscopy showed that the sugarcane bagasse extract might change cell morphology and internal structure.
25952848	3	41	theme	Listeria	487:494	arg1	monocytogenes					496:508	Listeria monocytogenes	487:508	Listeria monocytogenes	487:508	The sugarcane bagasse extract showed bacteriostatic activity against the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium.
25952848	6	42	theme	electron	923:930	arg1	microscopy					932:941	scanning electron microscopy	914:941	scanning electron microscopy	914:941	The results of scanning electron microscopy and transmission electron microscopy showed that the sugarcane bagasse extract might change cell morphology and internal structure.
25952848	5	43	theme	sulfate	738:744	arg1	electrophoresis					765:779	sodium dodecyl sulfate polyacrylamide gel electrophoresis	723:779	sodium dodecyl sulfate polyacrylamide gel electrophoresis	723:779	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	6	44	theme	scanning	914:921	arg1	microscopy					932:941	scanning electron microscopy	914:941	scanning electron microscopy	914:941	The results of scanning electron microscopy and transmission electron microscopy showed that the sugarcane bagasse extract might change cell morphology and internal structure.
25952848	1	45	theme	Sugarcane	125:133	arg1	bagasse					135:141	Sugarcane bagasse	125:141	Sugarcane bagasse	125:141	Sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth.
25952848	4	46	theme	bacterial	643:651	arg1	suspensions					658:668	bacterial cell suspensions	643:668	bacterial cell suspensions causing cellular leaking of electrolytes	643:709	Additionally, the sugarcane bagasse extract can increase the electric conductivity of bacterial cell suspensions causing cellular leaking of electrolytes.
25952848	5	47	theme	polyacrylamide	746:759	arg1	electrophoresis					765:779	sodium dodecyl sulfate polyacrylamide gel electrophoresis	723:779	sodium dodecyl sulfate polyacrylamide gel electrophoresis	723:779	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	1	48	theme	natural	152:158	arg1	compositions					160:171	natural compositions	152:171	natural compositions that can significantly inhibit food-borne pathogens growth	152:230	Sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth.
25952848	2	49	theme	dry	332:334	arg1	bagasse					336:342	higher than 4 mg/g dry bagasse	313:342	higher than 4 mg/g dry bagasse	313:342	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	2	49	theme	dry	332:334	arg1	content					268:274	the phenolic content	255:274	the phenolic content in sugarcane bagasse	255:295	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	5	50	dep	suggested	781:789	arg1	proteins					860:867	the damaged cellular proteins	839:867	the damaged cellular proteins	839:867	Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the antibacterial mechanism was probably due to the damaged cellular proteins by sugarcane bagasse extract.
25952848	2	51	theme	phenolic	259:266	arg1	bagasse					336:342	higher than 4 mg/g dry bagasse	313:342	higher than 4 mg/g dry bagasse	313:342	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	2	51	theme	phenolic	259:266	arg1	content					268:274	the phenolic content	255:274	the phenolic content in sugarcane bagasse	255:295	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	3	52	theme	aureus	479:484	arg1	growth					454:459	the growth	450:459	the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium	450:554	The sugarcane bagasse extract showed bacteriostatic activity against the growth of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salomonella typhimurium.
25952848	2	53	theme	quercetin/g	357:367	arg1	polyphenol					369:378	470 mg quercetin/g polyphenol	350:378	470 mg quercetin/g polyphenol	350:378	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	4	54	theme	suspensions	658:668	arg1	conductivity					627:638	the electric conductivity	614:638	the electric conductivity of bacterial cell suspensions causing cellular leaking of electrolytes	614:709	Additionally, the sugarcane bagasse extract can increase the electric conductivity of bacterial cell suspensions causing cellular leaking of electrolytes.
25952848	2	55	theme	mg	354:355	arg1	polyphenol					369:378	470 mg quercetin/g polyphenol	350:378	470 mg quercetin/g polyphenol	350:378	In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol.
25952848	4	56	theme	cell	653:656	arg1	suspensions					658:668	bacterial cell suspensions	643:668	bacterial cell suspensions causing cellular leaking of electrolytes	643:709	Additionally, the sugarcane bagasse extract can increase the electric conductivity of bacterial cell suspensions causing cellular leaking of electrolytes.
26304439	0	0	theme	bread	80:84	arg1	baking					60:65	baking	60:65	baking of model rye bread	60:84	Application of cross-linked and hydrolyzed arabinoxylans in baking of model rye bread.
26304439	1	1	theme	bread	232:236	arg1	formation					215:223	the structure formation	201:223	the structure formation of rye bread	201:236	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	0	2	theme	rye	76:78	arg1	bread					80:84	model rye bread	70:84	model rye bread	70:84	Application of cross-linked and hydrolyzed arabinoxylans in baking of model rye bread.
26304439	6	3	used	used	961:964	arg2	arabinoxylan					939:950	highly soluble, hydrolyzed arabinoxylan	912:950	highly soluble, hydrolyzed arabinoxylan	912:950	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	6	4	theme	rye	1021:1023	arg1	protein					1025:1031	rye protein	1021:1031	rye protein (3% or 6%)	1021:1042	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	6	5	theme	soluble	919:925	arg1	arabinoxylan					939:950	highly soluble, hydrolyzed arabinoxylan	912:950	highly soluble, hydrolyzed arabinoxylan	912:950	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	0	6	from	Application	0:10	arg1	baking					60:65	baking	60:65	baking of model rye bread	60:84	Application of cross-linked and hydrolyzed arabinoxylans in baking of model rye bread.
26304439	0	7	link	cross-linked	15:26	arg1	arabinoxylans					43:55	cross-linked and hydrolyzed arabinoxylans	15:55	cross-linked and hydrolyzed arabinoxylans	15:55	Application of cross-linked and hydrolyzed arabinoxylans in baking of model rye bread.
26304439	3	8	theme	typical	495:501	arg1	bread					507:511	typical rye bread	495:511	typical rye bread	495:511	It was observed that the applied mixes of these constituents result in a product closely resembling typical rye bread, even if arabinoxylan was modified (by cross-linking or hydrolysis).
26304439	2	9	theme	normal	287:292	arg1	flour					294:298	the normal flour	283:298	the normal flour	283:298	Instead of the normal flour, the dough contained starch, arabinoxylan and protein, which were isolated from rye wholemeal.
26304439	2	10	contain	contained	311:319	arg2	arabinoxylan					329:340	arabinoxylan	329:340	arabinoxylan	329:340	Instead of the normal flour, the dough contained starch, arabinoxylan and protein, which were isolated from rye wholemeal.
26304439	2	10	contain	contained	311:319	arg2	starch					321:326	starch	321:326	starch	321:326	Instead of the normal flour, the dough contained starch, arabinoxylan and protein, which were isolated from rye wholemeal.
26304439	2	10	contain	contained	311:319	arg1	dough					305:309	the dough	301:309	the dough	301:309	Instead of the normal flour, the dough contained starch, arabinoxylan and protein, which were isolated from rye wholemeal.
26304439	2	10	contain	contained	311:319	arg2	protein					346:352	protein	346:352	protein	346:352	Instead of the normal flour, the dough contained starch, arabinoxylan and protein, which were isolated from rye wholemeal.
26304439	3	11	theme	rye	503:505	arg1	bread					507:511	typical rye bread	495:511	typical rye bread	495:511	It was observed that the applied mixes of these constituents result in a product closely resembling typical rye bread, even if arabinoxylan was modified (by cross-linking or hydrolysis).
26304439	7	12	theme	decreased	1130:1138	arg1	viscosity					1140:1148	a decreased viscosity	1128:1148	a decreased viscosity of the dough	1128:1161	Further addition of arabinoxylan leads to excessive water absorption, resulting in a decreased viscosity of the dough during baking and insufficient gas retention.
26304439	7	13	theme	Further	1045:1051	arg1	addition					1053:1060	Further addition	1045:1060	Further addition of arabinoxylan	1045:1076	Further addition of arabinoxylan leads to excessive water absorption, resulting in a decreased viscosity of the dough during baking and insufficient gas retention.
26304439	6	14	theme	protein	1025:1031	arg1	amounts					1010:1016	larger amounts	1003:1016	larger amounts of rye protein (3% or 6%)	1003:1042	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	6	14	theme	protein	1025:1031	arg1	protein					1025:1031	rye protein	1021:1031	rye protein (3% or 6%)	1021:1042	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	1	15	with	arabinoxylan	117:128	arg1	structure					158:166	structure	158:166	structure	158:166	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	1	15	with	arabinoxylan	117:128	arg1	mass					149:152	varying molar mass	135:152	varying molar mass	135:152	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	7	16	theme	baking	1170:1175	arg1	retention					1198:1206	baking and insufficient gas retention	1170:1206	baking and insufficient gas retention	1170:1206	Further addition of arabinoxylan leads to excessive water absorption, resulting in a decreased viscosity of the dough during baking and insufficient gas retention.
26304439	4	17	theme	arabinoxylan	596:607	arg1	levels					586:591	The levels	582:591	The levels of arabinoxylan required for bread preparation	582:638	The levels of arabinoxylan required for bread preparation depended on its modification and mix composition.
26304439	5	18	theme	3	693:693	arg1	%					694:694	%	694:694	%	694:694	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	5	19	theme	maximum	709:715	arg1	level					728:732	the maximum applicable level	705:732	the maximum applicable level of poorly soluble cross-linked arabinoxylan	705:776	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	5	19	theme	maximum	709:715	arg1	%					783:783	3%	782:783	3%	782:783	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	5	20	theme	higher	789:794	arg1	amounts					796:802	higher amounts	789:802	higher amounts of this preparation	789:822	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	5	20	theme	higher	789:794	arg1	preparation					812:822	this preparation	807:822	this preparation	807:822	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	5	21	theme	%	694:694	arg1	protein					696:702	3% protein	693:702	3% protein	693:702	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	1	22	dep	mass	149:152	arg1	cross-linked					169:180	cross-linked	169:180	cross-linked	169:180	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	1	22	dep	mass	149:152	arg1	hydrolyzed					186:195	hydrolyzed	186:195	hydrolyzed	186:195	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	3	23	dep	modified	539:546	arg1	cross-linking					552:564	cross-linking	552:564	cross-linking	552:564	It was observed that the applied mixes of these constituents result in a product closely resembling typical rye bread, even if arabinoxylan was modified (by cross-linking or hydrolysis).
26304439	3	23	dep	modified	539:546	arg1	hydrolysis					569:578	hydrolysis	569:578	hydrolysis	569:578	It was observed that the applied mixes of these constituents result in a product closely resembling typical rye bread, even if arabinoxylan was modified (by cross-linking or hydrolysis).
26304439	5	24	theme	soluble	744:750	arg1	arabinoxylan					765:776	poorly soluble cross-linked arabinoxylan	737:776	poorly soluble cross-linked arabinoxylan	737:776	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	6	25	theme	other	901:905	arg1	hand					907:910	the other hand	897:910	the other hand	897:910	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	5	26	from	protein	696:702	arg1	%					783:783	3%	782:783	3%	782:783	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	5	26	from	protein	696:702	arg1	level					728:732	the maximum applicable level	705:732	the maximum applicable level of poorly soluble cross-linked arabinoxylan	705:776	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	7	27	theme	insufficient	1181:1192	arg1	retention					1198:1206	baking and insufficient gas retention	1170:1206	baking and insufficient gas retention	1170:1206	Further addition of arabinoxylan leads to excessive water absorption, resulting in a decreased viscosity of the dough during baking and insufficient gas retention.
26304439	5	28	theme	cross-linked	752:763	arg1	arabinoxylan					765:776	poorly soluble cross-linked arabinoxylan	737:776	poorly soluble cross-linked arabinoxylan	737:776	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	1	29	theme	model	259:263	arg1	bread					265:269	a model bread	257:269	a model bread	257:269	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	4	30	dep	modification	656:667	arg1	composition					677:687	composition	677:687	composition	677:687	The levels of arabinoxylan required for bread preparation depended on its modification and mix composition.
26304439	6	31	theme	higher	971:976	arg1	level					978:982	a higher level	969:982	a higher level (6%) together with larger amounts of rye protein (3% or 6%)	969:1042	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	6	31	theme	higher	971:976	arg1	%					986:986	6%	985:986	6%	985:986	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	0	32	theme	cross-linked	15:26	arg1	arabinoxylans					43:55	cross-linked and hydrolyzed arabinoxylans	15:55	cross-linked and hydrolyzed arabinoxylans	15:55	Application of cross-linked and hydrolyzed arabinoxylans in baking of model rye bread.
26304439	3	33	theme	applied	420:426	arg1	mixes					428:432	the applied mixes	416:432	the applied mixes of these constituents	416:454	It was observed that the applied mixes of these constituents result in a product closely resembling typical rye bread, even if arabinoxylan was modified (by cross-linking or hydrolysis).
26304439	0	34	theme	arabinoxylans	43:55	arg1	Application					0:10	Application	0:10	Application of cross-linked and hydrolyzed arabinoxylans in baking of model rye bread.	0:85	Application of cross-linked and hydrolyzed arabinoxylans in baking of model rye bread.
26304439	3	35	theme	constituents	443:454	arg1	mixes					428:432	the applied mixes	416:432	the applied mixes of these constituents	416:454	It was observed that the applied mixes of these constituents result in a product closely resembling typical rye bread, even if arabinoxylan was modified (by cross-linking or hydrolysis).
26304439	0	36	theme	hydrolyzed	32:41	arg1	arabinoxylans					43:55	cross-linked and hydrolyzed arabinoxylans	15:55	cross-linked and hydrolyzed arabinoxylans	15:55	Application of cross-linked and hydrolyzed arabinoxylans in baking of model rye bread.
26304439	5	37	theme	applicable	717:726	arg1	level					728:732	the maximum applicable level	705:732	the maximum applicable level of poorly soluble cross-linked arabinoxylan	705:776	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	5	37	theme	applicable	717:726	arg1	%					783:783	3%	782:783	3%	782:783	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	7	38	theme	gas	1194:1196	arg1	retention					1198:1206	baking and insufficient gas retention	1170:1206	baking and insufficient gas retention	1170:1206	Further addition of arabinoxylan leads to excessive water absorption, resulting in a decreased viscosity of the dough during baking and insufficient gas retention.
26304439	7	39	theme	dough	1157:1161	arg1	viscosity					1140:1148	a decreased viscosity	1128:1148	a decreased viscosity of the dough	1128:1161	Further addition of arabinoxylan leads to excessive water absorption, resulting in a decreased viscosity of the dough during baking and insufficient gas retention.
26304439	2	40	attach	isolated	366:373	arg2	arabinoxylan					329:340	arabinoxylan	329:340	arabinoxylan	329:340	Instead of the normal flour, the dough contained starch, arabinoxylan and protein, which were isolated from rye wholemeal.
26304439	2	40	attach	isolated	366:373	arg2	starch					321:326	starch	321:326	starch	321:326	Instead of the normal flour, the dough contained starch, arabinoxylan and protein, which were isolated from rye wholemeal.
26304439	2	40	attach	isolated	366:373	arg2	protein					346:352	protein	346:352	protein	346:352	Instead of the normal flour, the dough contained starch, arabinoxylan and protein, which were isolated from rye wholemeal.
26304439	2	40	attach	isolated	366:373	arg1	rye					380:382	rye wholemeal	380:392	rye wholemeal	380:392	Instead of the normal flour, the dough contained starch, arabinoxylan and protein, which were isolated from rye wholemeal.
26304439	6	41	theme	larger	1003:1008	arg1	amounts					1010:1016	larger amounts	1003:1016	larger amounts of rye protein (3% or 6%)	1003:1042	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	6	41	theme	larger	1003:1008	arg1	protein					1025:1031	rye protein	1021:1031	rye protein (3% or 6%)	1021:1042	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	1	42	theme	water	99:103	arg1	arabinoxylan					117:128	water extractable arabinoxylan	99:128	water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed)	99:196	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	6	43	dep	soluble	919:925	arg1	hydrolyzed					928:937	hydrolyzed	928:937	hydrolyzed	928:937	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	7	44	theme	excessive	1087:1095	arg1	absorption					1103:1112	excessive water absorption	1087:1112	excessive water absorption	1087:1112	Further addition of arabinoxylan leads to excessive water absorption, resulting in a decreased viscosity of the dough during baking and insufficient gas retention.
26304439	7	45	theme	arabinoxylan	1065:1076	arg1	addition					1053:1060	Further addition	1045:1060	Further addition of arabinoxylan	1045:1076	Further addition of arabinoxylan leads to excessive water absorption, resulting in a decreased viscosity of the dough during baking and insufficient gas retention.
26304439	1	46	theme	extractable	105:115	arg1	arabinoxylan					117:128	water extractable arabinoxylan	99:128	water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed)	99:196	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	1	47	from	role	91:94	arg1	formation					215:223	the structure formation	201:223	the structure formation of rye bread	201:236	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	5	48	link	cross-linked	752:763	arg1	arabinoxylan					765:776	poorly soluble cross-linked arabinoxylan	737:776	poorly soluble cross-linked arabinoxylan	737:776	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	1	49	theme	arabinoxylan	117:128	arg1	role					91:94	The role	87:94	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread	87:236	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	5	50	theme	bread	880:884	arg1	volume					886:891	bread volume	880:891	bread volume	880:891	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	1	51	theme	structure	205:213	arg1	formation					215:223	the structure formation	201:223	the structure formation of rye bread	201:236	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	5	52	theme	arabinoxylan	765:776	arg1	level					728:732	the maximum applicable level	705:732	the maximum applicable level of poorly soluble cross-linked arabinoxylan	705:776	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	5	52	theme	arabinoxylan	765:776	arg1	%					783:783	3%	782:783	3%	782:783	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	4	53	theme	bread	622:626	arg1	preparation					628:638	bread preparation	622:638	bread preparation	622:638	The levels of arabinoxylan required for bread preparation depended on its modification and mix composition.
26304439	5	54	theme	viscous	851:857	arg1	dough					859:863	an extensively viscous dough	836:863	an extensively viscous dough	836:863	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	0	55	theme	model	70:74	arg1	bread					80:84	model rye bread	70:84	model rye bread	70:84	Application of cross-linked and hydrolyzed arabinoxylans in baking of model rye bread.
26304439	5	56	theme	preparation	812:822	arg1	amounts					796:802	higher amounts	789:802	higher amounts of this preparation	789:822	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	5	56	theme	preparation	812:822	arg1	preparation					812:822	this preparation	807:822	this preparation	807:822	At 3% protein, the maximum applicable level of poorly soluble cross-linked arabinoxylan was 3%, as higher amounts of this preparation resulted in an extensively viscous dough and diminished bread volume.
26304439	6	57	dep	protein	1025:1031	arg1	%					1035:1035	3%	1034:1035	3%	1034:1035	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	6	57	dep	protein	1025:1031	arg1	%					1041:1041	6%	1040:1041	6%	1040:1041	On the other hand highly soluble, hydrolyzed arabinoxylan could be used at a higher level (6%) together with larger amounts of rye protein (3% or 6%).
26304439	1	58	theme	varying	135:141	arg1	mass					149:152	varying molar mass	135:152	varying molar mass	135:152	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	7	59	theme	water	1097:1101	arg1	absorption					1103:1112	excessive water absorption	1087:1112	excessive water absorption	1087:1112	Further addition of arabinoxylan leads to excessive water absorption, resulting in a decreased viscosity of the dough during baking and insufficient gas retention.
26304439	2	60	theme	wholemeal	384:392	arg1	rye					380:382	rye wholemeal	380:392	rye wholemeal	380:392	Instead of the normal flour, the dough contained starch, arabinoxylan and protein, which were isolated from rye wholemeal.
26304439	1	61	theme	molar	143:147	arg1	mass					149:152	varying molar mass	135:152	varying molar mass	135:152	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
26304439	1	62	theme	rye	228:230	arg1	bread					232:236	rye bread	228:236	rye bread	228:236	The role of water extractable arabinoxylan with varying molar mass and structure (cross-linked vs. hydrolyzed) in the structure formation of rye bread was examined using a model bread.
24189381	0	0	theme	life	94:97	arg1	assessment					105:114	life cycle assessment	94:114	life cycle assessment	94:114	Evaluating microalgal integrated biorefinery schemes: empirical controlled growth studies and life cycle assessment.
24189381	7	1	contain	had	1052:1054	arg2	impacts					1082:1088	the lowest eutrophication impacts	1056:1088	the lowest eutrophication impacts	1056:1088	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	7	1	contain	had	1052:1054	arg1	case					975:978	A marine, N-deplete case	955:978	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling	955:1050	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	8	2	theme	Tailoring	1091:1099	arg1	conditions					1122:1131	Tailoring algal species/growth conditions	1091:1131	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products	1091:1221	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products can enable a sustainable integrated microalgal biorefinery.
24189381	7	3	theme	nutrient	1033:1040	arg1	recycling					1042:1050	effective nutrient recycling	1023:1050	effective nutrient recycling	1023:1050	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	2	4	theme	eutrophication	544:557	arg1	potential					559:567	eutrophication potential	544:567	eutrophication potential	544:567	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	6	5	theme	Maximizing	860:869	arg1	productivity					883:894	Maximizing total lipid productivity	860:894	Maximizing total lipid productivity rather than lipid content	860:920	Maximizing total lipid productivity rather than lipid content yielded the least GHG emissions.
24189381	2	6	theme	cycle	337:341	arg1	assessment					343:352	A life cycle assessment	330:352	A life cycle assessment	330:352	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	8	7	theme	sustainable	1236:1246	arg1	biorefinery					1270:1280	a sustainable integrated microalgal biorefinery	1234:1280	a sustainable integrated microalgal biorefinery	1234:1280	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products can enable a sustainable integrated microalgal biorefinery.
24189381	8	8	theme	species/growth	1107:1120	arg1	conditions					1122:1131	Tailoring algal species/growth conditions	1091:1131	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products	1091:1221	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products can enable a sustainable integrated microalgal biorefinery.
24189381	7	9	dep	marine	957:962	arg1	N-deplete					965:973	N-deplete	965:973	N-deplete	965:973	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	1	10	theme	biomolecular	272:283	arg1	starch					301:306	starch	301:306	starch	301:306	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	1	10	theme	biomolecular	272:283	arg1	lipid					322:326	lipid	322:326	lipid	322:326	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	1	10	theme	biomolecular	272:283	arg1	protein					309:315	protein	309:315	protein	309:315	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	1	10	theme	biomolecular	272:283	arg1	fractions					285:293	biomolecular fractions	272:293	biomolecular fractions (i.e. starch, protein, and lipid)	272:327	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	0	11	theme	cycle	99:103	arg1	assessment					105:114	life cycle assessment	94:114	life cycle assessment	94:114	Evaluating microalgal integrated biorefinery schemes: empirical controlled growth studies and life cycle assessment.
24189381	8	12	theme	algal	1101:1105	arg1	conditions					1122:1131	Tailoring algal species/growth conditions	1091:1131	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products	1091:1221	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products can enable a sustainable integrated microalgal biorefinery.
24189381	7	13	theme	low	996:998	arg1	productivity					1006:1017	relatively low lipid productivity	985:1017	relatively low lipid productivity	985:1017	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	8	14	theme	microalgal	1259:1268	arg1	biorefinery					1270:1280	a sustainable integrated microalgal biorefinery	1234:1280	a sustainable integrated microalgal biorefinery	1234:1280	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products can enable a sustainable integrated microalgal biorefinery.
24189381	6	15	theme	GHG	940:942	arg1	emissions					944:952	the least GHG emissions	930:952	the least GHG emissions	930:952	Maximizing total lipid productivity rather than lipid content yielded the least GHG emissions.
24189381	5	16	theme	carbon	838:843	arg1	sequestration					845:857	carbon sequestration	838:857	carbon sequestration	838:857	Composition of the non-lipid fraction presented significant trade-offs among biogas production, animal feed substitution, nutrient recycling, and carbon sequestration.
24189381	2	17	theme	species/growth	388:401	arg1	conditions					403:412	varying species/growth conditions	380:412	varying species/growth conditions	380:412	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	8	18	theme	integrated	1248:1257	arg1	biorefinery					1270:1280	a sustainable integrated microalgal biorefinery	1234:1280	a sustainable integrated microalgal biorefinery	1234:1280	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products can enable a sustainable integrated microalgal biorefinery.
24189381	6	19	theme	least	934:938	arg1	emissions					944:952	the least GHG emissions	930:952	the least GHG emissions	930:952	Maximizing total lipid productivity rather than lipid content yielded the least GHG emissions.
24189381	5	20	theme	nutrient	814:821	arg1	recycling					823:831	nutrient recycling	814:831	nutrient recycling	814:831	Composition of the non-lipid fraction presented significant trade-offs among biogas production, animal feed substitution, nutrient recycling, and carbon sequestration.
24189381	8	21	theme	fractions	1169:1177	arg1	mix					1149:1151	the mix	1145:1151	the mix of biomolecular fractions matched to desired products and co-products	1145:1221	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products can enable a sustainable integrated microalgal biorefinery.
24189381	1	22	theme	nitrogen	183:190	arg1	replete					192:198	nitrogen replete	183:198	nitrogen replete	183:198	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	0	23	theme	microalgal	11:20	arg1	schemes					45:51	microalgal integrated biorefinery schemes	11:51	microalgal integrated biorefinery schemes	11:51	Evaluating microalgal integrated biorefinery schemes: empirical controlled growth studies and life cycle assessment.
24189381	8	24	dep	conditions	1122:1131	arg1	optimize					1136:1143	optimize	1136:1143	to optimize the mix of biomolecular fractions matched to desired products and co-products	1133:1221	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products can enable a sustainable integrated microalgal biorefinery.
24189381	5	25	theme	animal	788:793	arg1	substitution					800:811	animal feed substitution	788:811	animal feed substitution	788:811	Composition of the non-lipid fraction presented significant trade-offs among biogas production, animal feed substitution, nutrient recycling, and carbon sequestration.
24189381	2	26	theme	final	452:456	arg1	substitution					466:477	final product substitution	452:477	final product substitution	452:477	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	0	27	theme	biorefinery	33:43	arg1	schemes					45:51	microalgal integrated biorefinery schemes	11:51	microalgal integrated biorefinery schemes	11:51	Evaluating microalgal integrated biorefinery schemes: empirical controlled growth studies and life cycle assessment.
24189381	7	28	with	case	975:978	arg1	recycling					1042:1050	effective nutrient recycling	1023:1050	effective nutrient recycling	1023:1050	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	7	28	with	case	975:978	arg1	productivity					1006:1017	relatively low lipid productivity	985:1017	relatively low lipid productivity	985:1017	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	5	29	theme	fraction	721:728	arg1	Composition					692:702	Composition	692:702	Composition of the non-lipid fraction	692:728	Composition of the non-lipid fraction presented significant trade-offs among biogas production, animal feed substitution, nutrient recycling, and carbon sequestration.
24189381	7	30	theme	lipid	1000:1004	arg1	productivity					1006:1017	relatively low lipid productivity	985:1017	relatively low lipid productivity	985:1017	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	2	31	theme	biomass	431:437	arg1	fraction					439:446	each biomass fraction	426:446	each biomass fraction	426:446	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	0	32	theme	integrated	22:31	arg1	schemes					45:51	microalgal integrated biorefinery schemes	11:51	microalgal integrated biorefinery schemes	11:51	Evaluating microalgal integrated biorefinery schemes: empirical controlled growth studies and life cycle assessment.
24189381	1	33	from	fractions	285:293	arg1	yield					262:266	total biomass yield	248:266	total biomass yield	248:266	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	7	34	theme	lowest	1060:1065	arg1	impacts					1082:1088	the lowest eutrophication impacts	1056:1088	the lowest eutrophication impacts	1056:1088	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	2	35	theme	energy	488:493	arg1	consumption					495:505	energy consumption	488:505	energy consumption	488:505	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	5	36	theme	significant	740:750	arg1	trade-offs					752:761	significant trade-offs	740:761	significant trade-offs among biogas production, animal feed substitution, nutrient recycling, and carbon sequestration	740:857	Composition of the non-lipid fraction presented significant trade-offs among biogas production, animal feed substitution, nutrient recycling, and carbon sequestration.
24189381	8	37	theme	biomolecular	1156:1167	arg1	fractions					1169:1177	biomolecular fractions	1156:1177	biomolecular fractions matched to desired products and co-products	1156:1221	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products can enable a sustainable integrated microalgal biorefinery.
24189381	5	38	theme	biogas	769:774	arg1	production					776:785	biogas production	769:785	biogas production	769:785	Composition of the non-lipid fraction presented significant trade-offs among biogas production, animal feed substitution, nutrient recycling, and carbon sequestration.
24189381	4	39	theme	Protein	626:632	arg1	fractions					651:659	Protein and carbohydrate fractions	626:659	Protein and carbohydrate fractions	626:659	Protein and carbohydrate fractions were processed as co-products.
24189381	4	39	theme	Protein	626:632	arg1	co-products					679:689	co-products	679:689	co-products	679:689	Protein and carbohydrate fractions were processed as co-products.
24189381	7	40	theme	eutrophication	1067:1080	arg1	impacts					1082:1088	the lowest eutrophication impacts	1056:1088	the lowest eutrophication impacts	1056:1088	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	0	41	theme	controlled	64:73	arg1	studies					82:88	empirical controlled growth studies	54:88	empirical controlled growth studies	54:88	Evaluating microalgal integrated biorefinery schemes: empirical controlled growth studies and life cycle assessment.
24189381	2	42	theme	greenhouse	508:517	arg1	GHG					534:536	GHG	534:536	GHG	534:536	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	2	42	theme	greenhouse	508:517	arg1	emissions					523:531	greenhouse gas emissions	508:531	greenhouse gas emissions (GHG)	508:537	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	2	43	theme	life	332:335	arg1	assessment					343:352	A life cycle assessment	330:352	A life cycle assessment	330:352	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	0	44	theme	empirical	54:62	arg1	studies					82:88	empirical controlled growth studies	54:88	empirical controlled growth studies	54:88	Evaluating microalgal integrated biorefinery schemes: empirical controlled growth studies and life cycle assessment.
24189381	6	45	theme	lipid	908:912	arg1	content					914:920	lipid content	908:920	Maximizing total lipid productivity rather than lipid content	860:920	Maximizing total lipid productivity rather than lipid content yielded the least GHG emissions.
24189381	7	46	theme	marine	957:962	arg1	case					975:978	A marine, N-deplete case	955:978	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling	955:1050	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	1	47	dep	conditions	212:221	arg1	deplete					204:210	deplete	204:210	deplete	204:210	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	1	48	from	impact	238:243	arg1	yield					262:266	total biomass yield	248:266	total biomass yield	248:266	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	1	49	dep	fractions	285:293	arg1	starch					301:306	starch	301:306	starch	301:306	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	1	49	dep	fractions	285:293	arg1	fractions					285:293	biomolecular fractions	272:293	biomolecular fractions (i.e. starch, protein, and lipid)	272:327	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	1	49	dep	fractions	285:293	arg1	protein					309:315	protein	309:315	protein	309:315	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	1	49	dep	fractions	285:293	arg1	lipid					322:326	lipid	322:326	lipid	322:326	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	5	50	theme	feed	795:798	arg1	substitution					800:811	animal feed substitution	788:811	animal feed substitution	788:811	Composition of the non-lipid fraction presented significant trade-offs among biogas production, animal feed substitution, nutrient recycling, and carbon sequestration.
24189381	5	51	theme	non-lipid	711:719	arg1	fraction					721:728	the non-lipid fraction	707:728	the non-lipid fraction	707:728	Composition of the non-lipid fraction presented significant trade-offs among biogas production, animal feed substitution, nutrient recycling, and carbon sequestration.
24189381	6	52	theme	lipid	877:881	arg1	productivity					883:894	Maximizing total lipid productivity	860:894	Maximizing total lipid productivity rather than lipid content	860:920	Maximizing total lipid productivity rather than lipid content yielded the least GHG emissions.
24189381	1	53	theme	marine	140:145	arg1	species					158:164	Two freshwater and two marine microalgae species	117:164	species	158:164	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	2	54	theme	gas	519:521	arg1	GHG					534:536	GHG	534:536	GHG	534:536	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	2	54	theme	gas	519:521	arg1	emissions					523:531	greenhouse gas emissions	508:531	greenhouse gas emissions (GHG)	508:537	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	6	55	theme	total	871:875	arg1	productivity					883:894	Maximizing total lipid productivity	860:894	Maximizing total lipid productivity rather than lipid content	860:920	Maximizing total lipid productivity rather than lipid content yielded the least GHG emissions.
24189381	3	56	theme	primary	609:615	arg1	product					617:623	the primary product	605:623	the primary product	605:623	Lipid for biodiesel was assumed as the primary product.
24189381	3	56	theme	primary	609:615	arg1	Lipid					570:574	Lipid	570:574	Lipid for biodiesel	570:588	Lipid for biodiesel was assumed as the primary product.
24189381	4	57	theme	carbohydrate	638:649	arg1	fractions					651:659	Protein and carbohydrate fractions	626:659	Protein and carbohydrate fractions	626:659	Protein and carbohydrate fractions were processed as co-products.
24189381	4	57	theme	carbohydrate	638:649	arg1	co-products					679:689	co-products	679:689	co-products	679:689	Protein and carbohydrate fractions were processed as co-products.
24189381	1	58	theme	microalgae	147:156	arg1	species					158:164	Two freshwater and two marine microalgae species	117:164	species	158:164	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	2	59	theme	varying	380:386	arg1	conditions					403:412	varying species/growth conditions	380:412	varying species/growth conditions	380:412	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	1	60	theme	total	248:252	arg1	yield					262:266	total biomass yield	248:266	total biomass yield	248:266	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	0	61	theme	growth	75:80	arg1	studies					82:88	empirical controlled growth studies	54:88	empirical controlled growth studies	54:88	Evaluating microalgal integrated biorefinery schemes: empirical controlled growth studies and life cycle assessment.
24189381	1	62	dep	starch	301:306	arg1	i.e.					296:299	i.e.	296:299	i.e.	296:299	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
24189381	0	63	dep	Evaluating	0:9	arg1	assessment					105:114	life cycle assessment	94:114	life cycle assessment	94:114	Evaluating microalgal integrated biorefinery schemes: empirical controlled growth studies and life cycle assessment.
24189381	0	63	dep	Evaluating	0:9	arg1	studies					82:88	empirical controlled growth studies	54:88	empirical controlled growth studies	54:88	Evaluating microalgal integrated biorefinery schemes: empirical controlled growth studies and life cycle assessment.
24189381	7	64	theme	effective	1023:1031	arg1	recycling					1042:1050	effective nutrient recycling	1023:1050	effective nutrient recycling	1023:1050	A marine, N-deplete case with relatively low lipid productivity but effective nutrient recycling had the lowest eutrophication impacts.
24189381	2	65	theme	product	458:464	arg1	substitution					466:477	final product substitution	452:477	final product substitution	452:477	A life cycle assessment was performed to evaluate varying species/growth conditions considering each biomass fraction and final product substitution based on energy consumption, greenhouse gas emissions (GHG), and eutrophication potential.
24189381	8	66	theme	desired	1190:1196	arg1	products					1198:1205	desired products	1190:1205	desired products	1190:1205	Tailoring algal species/growth conditions to optimize the mix of biomolecular fractions matched to desired products and co-products can enable a sustainable integrated microalgal biorefinery.
24189381	1	67	theme	biomass	254:260	arg1	yield					262:266	total biomass yield	248:266	total biomass yield	248:266	Two freshwater and two marine microalgae species were grown under nitrogen replete and deplete conditions evaluating the impact on total biomass yield and biomolecular fractions (i.e. starch, protein, and lipid).
27600967	7	0	theme	samples	1383:1389	arg1	Collection					1360:1369	Collection	1360:1369	Collection of two milk samples only per cow	1360:1402	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	4	1	theme	December	784:791	arg1	2011					793:796	December 2011	784:796	December 2011	784:796	The Gold Standard protocol was applied on five commercial dairy farms with AMS, between December 2011 and February 2013.
27600967	3	2	theme	optimum	608:614	arg1	interval					631:638	the optimum milk recording interval	604:638	the optimum milk recording interval	604:638	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	2	3	theme	recording	322:330	arg1	intervals					332:340	milk recording intervals	317:340	milk recording intervals used for herd testing indoor fed cows	317:378	Therefore, milk recording intervals used for herd testing indoor fed cows may not be suitable for cows on pasture based farms.
27600967	5	4	theme	shorter	1110:1116	arg1	periods					1127:1133	shorter sampling periods	1110:1133	six shorter sampling periods	1106:1133	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	7	5	from	optimal	1408:1414	arg1	terms					1419:1423	terms	1419:1423	terms of high concordance correlation coefficients for milk volume and components	1419:1499	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	7	5	from	optimal	1408:1414	arg1	proportion					1511:1520	a low proportion	1505:1520	a low proportion of missed cow-test days	1505:1544	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	1	6	theme	milking	247:253	arg1	frequencies					255:265	lower milking frequencies	241:265	lower milking frequencies than indoor fed cows milked using AMS	241:303	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	7	7	from	terms	1419:1423	arg1	optimal					1408:1414	optimal	1408:1414	optimal	1408:1414	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	8	8	theme	samples	1751:1757	arg1	number					1741:1746	a fixed number	1733:1746	a fixed number of samples	1733:1757	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	0	9	theme	Shorter	0:6	arg1	periods					17:23	Shorter sampling periods	0:23	Shorter sampling periods	0:23	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	8	10	theme	diurnal	1604:1610	arg1	variations					1612:1621	diurnal variations	1604:1621	diurnal variations in milk composition	1604:1641	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	3	11	theme	milk	530:533	arg1	intervals					545:553	milk recording intervals	530:553	milk recording intervals of less than the Gold Standard (48 hs)	530:592	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	1	12	theme	pasture	179:185	arg1	cows					166:169	Dairy cows	160:169	Dairy cows grazing pasture and milked using automated milking systems (AMS)	160:234	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	7	13	theme	cow-test	1532:1539	arg1	days					1541:1544	missed cow-test days	1525:1544	missed cow-test days	1525:1544	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	3	14	theme	herd	660:663	arg1	average					665:671	the herd average	656:671	the herd average for milking frequency	656:693	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	6	15	theme	48	1225:1226	arg1	h					1228:1228	h	1228:1228	h	1228:1228	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	3	16	theme	24	476:477	arg1	h					479:479	h	479:479	h	479:479	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	1	17	theme	milked	191:196	arg1	cows					166:169	Dairy cows	160:169	Dairy cows grazing pasture and milked using automated milking systems (AMS)	160:234	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	9	18	theme	study	1809:1813	arg1	results					1793:1799	the results	1789:1799	the results of this study	1789:1813	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	3	19	theme	less	558:561	arg1	intervals					545:553	milk recording intervals	530:553	milk recording intervals of less than the Gold Standard (48 hs)	530:592	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	8	20	theme	standardised	1646:1657	arg1	estimates					1664:1672	standardised 24 h estimates	1646:1672	standardised 24 h estimates for milk volume and components	1646:1703	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	1	21	theme	automated	204:212	arg1	AMS					231:233	AMS	231:233	AMS	231:233	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	1	21	theme	automated	204:212	arg1	systems					222:228	automated milking systems	204:228	automated milking systems (AMS)	204:234	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	7	22	theme	low	1507:1509	arg1	proportion					1511:1520	a low proportion	1505:1520	a low proportion of missed cow-test days	1505:1544	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	6	23	theme	pasture	1335:1341	arg1	farms					1353:1357	pasture based AMS farms	1335:1357	pasture based AMS farms	1335:1357	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	0	24	theme	automated	133:141	arg1	systems					151:157	automated milking systems	133:157	automated milking systems	133:157	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	5	25	theme	milk	938:941	arg1	volume					943:948	milk volume	938:948	milk volume	938:948	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	8	26	theme	h	1662:1662	arg1	estimates					1664:1672	standardised 24 h estimates	1646:1672	standardised 24 h estimates for milk volume and components	1646:1703	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	5	27	theme	milk	825:828	arg1	periods					845:851	12 milk recording test periods	822:851	12 milk recording test periods	822:851	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	5	28	theme	sampling	1118:1125	arg1	periods					1127:1133	shorter sampling periods	1110:1133	six shorter sampling periods	1106:1133	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	5	29	theme	samples	1177:1183	arg1	number					1162:1167	a fixed number	1154:1167	a fixed number of milk samples per cow	1154:1191	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	5	30	theme	test	840:843	arg1	periods					845:851	12 milk recording test periods	822:851	12 milk recording test periods	822:851	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	9	31	theme	split	1842:1846	arg1	protocol					1848:1855	a split protocol	1840:1855	a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015)	1840:1958	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	0	32	theme	volume	56:61	arg1	periods					17:23	Shorter sampling periods	0:23	Shorter sampling periods	0:23	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	0	32	theme	volume	56:61	arg1	estimates					38:46	accurate estimates	29:46	accurate estimates	29:46	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	7	33	theme	milk	1474:1477	arg1	volume					1479:1484	milk volume	1474:1484	milk volume	1474:1484	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	5	34	theme	fixed	1156:1160	arg1	number					1162:1167	a fixed number	1154:1167	a fixed number of milk samples per cow	1154:1191	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	0	35	theme	components	67:76	arg1	periods					17:23	Shorter sampling periods	0:23	Shorter sampling periods	0:23	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	0	35	theme	components	67:76	arg1	estimates					38:46	accurate estimates	29:46	accurate estimates	29:46	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	7	36	theme	coefficients	1457:1468	arg1	terms					1419:1423	terms	1419:1423	terms of high concordance correlation coefficients for milk volume and components	1419:1499	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	7	36	theme	coefficients	1457:1468	arg1	proportion					1511:1520	a low proportion	1505:1520	a low proportion of missed cow-test days	1505:1544	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	2	37	from	cows	404:407	arg1	farms					426:430	pasture based farms	412:430	pasture based farms	412:430	Therefore, milk recording intervals used for herd testing indoor fed cows may not be suitable for cows on pasture based farms.
27600967	7	38	theme	concordance	1433:1443	arg1	coefficients					1457:1468	high concordance correlation coefficients	1428:1468	high concordance correlation coefficients for milk volume and components	1428:1499	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	4	39	theme	Gold	700:703	arg1	protocol					714:721	The Gold Standard protocol	696:721	The Gold Standard protocol	696:721	The Gold Standard protocol was applied on five commercial dairy farms with AMS, between December 2011 and February 2013.
27600967	2	40	theme	fed	371:373	arg1	cows					375:378	herd testing indoor fed cows	351:378	herd testing indoor fed cows	351:378	Therefore, milk recording intervals used for herd testing indoor fed cows may not be suitable for cows on pasture based farms.
27600967	0	41	theme	pasture	95:101	arg1	herds					115:119	pasture based dairy herds	95:119	pasture based dairy herds milked with automated milking systems	95:157	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	5	42	theme	Standard	1000:1007	arg1	protocol					1009:1016	the Gold Standard protocol	991:1016	the Gold Standard protocol	991:1016	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	9	43	theme	average	1887:1893	arg1	frequency					1903:1911	the average milking frequency	1883:1911	the average milking frequency for the herd (NZ Herd Test Standard 8100:2015)	1883:1958	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	2	44	theme	testing	356:362	arg1	cows					375:378	herd testing indoor fed cows	351:378	herd testing indoor fed cows	351:378	Therefore, milk recording intervals used for herd testing indoor fed cows may not be suitable for cows on pasture based farms.
27600967	3	45	with	herds	519:523	arg1	intervals					545:553	milk recording intervals	530:553	milk recording intervals of less than the Gold Standard (48 hs)	530:592	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	5	46	theme	sampling	1076:1083	arg1	scenarios					1085:1093	nine alternative sampling scenarios	1059:1093	nine alternative sampling scenarios	1059:1093	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	5	46	theme	sampling	1076:1083	arg1	periods					1127:1133	shorter sampling periods	1110:1133	six shorter sampling periods	1106:1133	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	5	46	theme	sampling	1076:1083	arg1	three					1139:1143	three	1139:1143	three	1139:1143	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	1	47	theme	indoor	272:277	arg1	cows					283:286	indoor fed cows	272:286	indoor fed cows milked using AMS	272:303	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	7	48	theme	days	1541:1544	arg1	terms					1419:1423	terms	1419:1423	terms of high concordance correlation coefficients for milk volume and components	1419:1499	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	7	48	theme	days	1541:1544	arg1	proportion					1511:1520	a low proportion	1505:1520	a low proportion of missed cow-test days	1505:1544	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	0	49	theme	accurate	29:36	arg1	estimates					38:46	accurate estimates	29:46	accurate estimates	29:46	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	5	50	theme	h	922:922	arg1	estimates					924:932	standardised 24 h estimates	906:932	standardised 24 h estimates for milk volume and milk composition	906:969	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	2	51	theme	based	420:424	arg1	farms					426:430	pasture based farms	412:430	pasture based farms	412:430	Therefore, milk recording intervals used for herd testing indoor fed cows may not be suitable for cows on pasture based farms.
27600967	4	52	theme	dairy	754:758	arg1	farms					760:764	five commercial dairy farms	738:764	five commercial dairy farms with AMS, between December 2011 and February 2013	738:814	The Gold Standard protocol was applied on five commercial dairy farms with AMS, between December 2011 and February 2013.
27600967	2	53	theme	milk	317:320	arg1	intervals					332:340	milk recording intervals	317:340	milk recording intervals used for herd testing indoor fed cows	317:378	Therefore, milk recording intervals used for herd testing indoor fed cows may not be suitable for cows on pasture based farms.
27600967	6	54	theme	accurate	1291:1298	arg1	estimates					1300:1308	accurate estimates	1291:1308	accurate estimates	1291:1308	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	9	55	theme	Herd	1930:1933	arg1	herd					1921:1924	the herd	1917:1924	the herd (NZ Herd Test Standard 8100:2015)	1917:1958	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	9	55	theme	Herd	1930:1933	arg1	8100:2015					1949:1957	NZ Herd Test Standard 8100:2015	1927:1957	NZ Herd Test Standard 8100:2015	1927:1957	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	8	56	from	composition	1631:1641	arg1	effects					1593:1599	the effects	1589:1599	the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components	1589:1703	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	1	57	theme	milking	214:220	arg1	AMS					231:233	AMS	231:233	AMS	231:233	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	1	57	theme	milking	214:220	arg1	systems					222:228	automated milking systems	204:228	automated milking systems (AMS)	204:234	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	9	58	theme	Standard	1940:1947	arg1	herd					1921:1924	the herd	1917:1924	the herd (NZ Herd Test Standard 8100:2015)	1917:1958	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	9	58	theme	Standard	1940:1947	arg1	8100:2015					1949:1957	NZ Herd Test Standard 8100:2015	1927:1957	NZ Herd Test Standard 8100:2015	1927:1957	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	3	59	theme	h	479:479	arg1	estimates					481:489	accurate standardised 24 h estimates	454:489	accurate standardised 24 h estimates	454:489	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	8	60	theme	Further	1547:1553	arg1	research					1555:1562	Further research	1547:1562	Further research	1547:1562	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	7	61	theme	milk	1378:1381	arg1	samples					1383:1389	two milk samples only	1374:1394	two milk samples only per cow	1374:1402	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	5	62	theme	standardised	906:917	arg1	estimates					924:932	standardised 24 h estimates	906:932	standardised 24 h estimates for milk volume and milk composition	906:969	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	8	63	theme	fixed	1735:1739	arg1	number					1741:1746	a fixed number	1733:1746	a fixed number of samples	1733:1757	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	3	64	theme	standardised	463:474	arg1	estimates					481:489	accurate standardised 24 h estimates	454:489	accurate standardised 24 h estimates	454:489	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	3	65	theme	milk	616:619	arg1	interval					631:638	the optimum milk recording interval	604:638	the optimum milk recording interval	604:638	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	8	66	from	effects	1593:1599	arg1	estimates					1664:1672	standardised 24 h estimates	1646:1672	standardised 24 h estimates for milk volume and components	1646:1703	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	8	66	from	effects	1593:1599	arg1	composition					1631:1641	milk composition	1626:1641	milk composition	1626:1641	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	6	67	theme	AMS	1349:1351	arg1	farms					1353:1357	pasture based AMS farms	1335:1357	pasture based AMS farms	1335:1357	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	1	68	theme	lower	241:245	arg1	frequencies					255:265	lower milking frequencies	241:265	lower milking frequencies than indoor fed cows milked using AMS	241:303	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	1	69	theme	Dairy	160:164	arg1	cows					166:169	Dairy cows	160:169	Dairy cows grazing pasture and milked using automated milking systems (AMS)	160:234	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	7	70	theme	missed	1525:1530	arg1	days					1541:1544	missed cow-test days	1525:1544	missed cow-test days	1525:1544	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	6	71	theme	h	1228:1228	arg1	protocol					1245:1252	a 48 h milk recording protocol	1223:1252	a 48 h milk recording protocol	1223:1252	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	6	71	theme	h	1228:1228	arg1	long					1271:1274	long	1271:1274	long	1271:1274	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	3	72	theme	AMS	515:517	arg1	herds					519:523	AMS herds	515:523	AMS herds with milk recording intervals of less than the Gold Standard (48 hs)	515:592	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	8	73	theme	variations	1612:1621	arg1	effects					1593:1599	the effects	1589:1599	the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components	1589:1703	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	6	74	from	recording	1322:1330	arg1	farms					1353:1357	pasture based AMS farms	1335:1357	pasture based AMS farms	1335:1357	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	8	75	theme	milk	1626:1629	arg1	composition					1631:1641	milk composition	1626:1641	milk composition	1626:1641	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	9	76	theme	New	1815:1817	arg1	Zealand					1819:1825	New Zealand	1815:1825	New Zealand	1815:1825	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	0	77	theme	milking	143:149	arg1	systems					151:157	automated milking systems	133:157	automated milking systems	133:157	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	3	78	theme	recording	535:543	arg1	intervals					545:553	milk recording intervals	530:553	milk recording intervals of less than the Gold Standard (48 hs)	530:592	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	4	79	theme	February	802:809	arg1	2013					811:814	February 2013	802:814	February 2013	802:814	The Gold Standard protocol was applied on five commercial dairy farms with AMS, between December 2011 and February 2013.
27600967	5	80	theme	milk	1172:1175	arg1	samples					1177:1183	milk samples	1172:1183	milk samples	1172:1183	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	8	81	theme	24	1659:1660	arg1	h					1662:1662	h	1662:1662	h	1662:1662	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	5	82	theme	recording	830:838	arg1	periods					845:851	12 milk recording test periods	822:851	12 milk recording test periods	822:851	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	5	83	theme	milk	954:957	arg1	composition					959:969	milk composition	954:969	milk composition	954:969	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	1	84	contain	have	236:239	arg2	frequencies					255:265	lower milking frequencies	241:265	lower milking frequencies than indoor fed cows milked using AMS	241:303	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	1	84	contain	have	236:239	arg1	cows					166:169	Dairy cows	160:169	Dairy cows grazing pasture and milked using automated milking systems (AMS)	160:234	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	8	85	theme	milk	1678:1681	arg1	volume					1683:1688	milk volume	1678:1688	milk volume	1678:1688	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	7	86	theme	correlation	1445:1455	arg1	coefficients					1457:1468	high concordance correlation coefficients	1428:1468	high concordance correlation coefficients for milk volume and components	1428:1499	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	0	87	theme	dairy	109:113	arg1	herds					115:119	pasture based dairy herds	95:119	pasture based dairy herds milked with automated milking systems	95:157	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	9	88	theme	herd	1861:1864	arg1	testing					1866:1872	herd testing	1861:1872	herd testing	1861:1872	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	5	89	theme	cow-test	869:876	arg1	days					878:881	2211 cow-test days	864:881	2211 cow-test days	864:881	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	4	90	with	farms	760:764	arg1	AMS					771:773	AMS	771:773	AMS	771:773	The Gold Standard protocol was applied on five commercial dairy farms with AMS, between December 2011 and February 2013.
27600967	7	91	theme	high	1428:1431	arg1	coefficients					1457:1468	high concordance correlation coefficients	1428:1468	high concordance correlation coefficients for milk volume and components	1428:1499	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	6	92	theme	recording	1235:1243	arg1	protocol					1245:1252	a 48 h milk recording protocol	1223:1252	a 48 h milk recording protocol	1223:1252	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	6	92	theme	recording	1235:1243	arg1	long					1271:1274	long	1271:1274	long	1271:1274	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	5	93	theme	Gold	995:998	arg1	protocol					1009:1016	the Gold Standard protocol	991:1016	the Gold Standard protocol	991:1016	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	7	94	from	proportion	1511:1520	arg1	optimal					1408:1414	optimal	1408:1414	optimal	1408:1414	Collection of two milk samples only per cow was optimal in terms of high concordance correlation coefficients for milk volume and components and a low proportion of missed cow-test days.
27600967	0	95	theme	based	103:107	arg1	herds					115:119	pasture based dairy herds	95:119	pasture based dairy herds milked with automated milking systems	95:157	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	0	96	theme	sampling	8:15	arg1	periods					17:23	Shorter sampling periods	0:23	Shorter sampling periods	0:23	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	9	97	theme	milking	1895:1901	arg1	frequency					1903:1911	the average milking frequency	1883:1911	the average milking frequency for the herd (NZ Herd Test Standard 8100:2015)	1883:1958	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	2	98	theme	herd	351:354	arg1	cows					375:378	herd testing indoor fed cows	351:378	herd testing indoor fed cows	351:378	Therefore, milk recording intervals used for herd testing indoor fed cows may not be suitable for cows on pasture based farms.
27600967	6	99	theme	milk	1317:1320	arg1	recording					1322:1330	milk recording	1317:1330	milk recording on pasture based AMS farms	1317:1357	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	1	100	theme	fed	279:281	arg1	cows					283:286	indoor fed cows	272:286	indoor fed cows milked using AMS	272:303	Dairy cows grazing pasture and milked using automated milking systems (AMS) have lower milking frequencies than indoor fed cows milked using AMS.
27600967	5	101	theme	24	919:920	arg1	h					922:922	h	922:922	h	922:922	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	6	102	theme	milk	1230:1233	arg1	protocol					1245:1252	a 48 h milk recording protocol	1223:1252	a 48 h milk recording protocol	1223:1252	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	6	102	theme	milk	1230:1233	arg1	long					1271:1274	long	1271:1274	long	1271:1274	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
27600967	0	103	theme	milk	51:54	arg1	volume					56:61	milk volume	51:61	milk volume	51:61	Shorter sampling periods and accurate estimates of milk volume and components are possible for pasture based dairy herds milked with automated milking systems.
27600967	2	104	theme	pasture	412:418	arg1	farms					426:430	pasture based farms	412:430	pasture based farms	412:430	Therefore, milk recording intervals used for herd testing indoor fed cows may not be suitable for cows on pasture based farms.
27600967	4	105	theme	commercial	743:752	arg1	farms					760:764	five commercial dairy farms	738:764	five commercial dairy farms with AMS, between December 2011 and February 2013	738:814	The Gold Standard protocol was applied on five commercial dairy farms with AMS, between December 2011 and February 2013.
27600967	3	106	theme	accurate	454:461	arg1	estimates					481:489	accurate standardised 24 h estimates	454:489	accurate standardised 24 h estimates	454:489	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	5	107	theme	alternative	1064:1074	arg1	scenarios					1085:1093	nine alternative sampling scenarios	1059:1093	nine alternative sampling scenarios	1059:1093	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	5	107	theme	alternative	1064:1074	arg1	periods					1127:1133	shorter sampling periods	1110:1133	six shorter sampling periods	1106:1133	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	5	107	theme	alternative	1064:1074	arg1	three					1139:1143	three	1139:1143	three	1139:1143	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	5	108	theme	cow	892:894	arg1	milkings					896:903	8049 cow milkings	887:903	8049 cow milkings	887:903	From 12 milk recording test periods, involving 2211 cow-test days and 8049 cow milkings, standardised 24 h estimates for milk volume and milk composition were calculated for the Gold Standard protocol and compared with those collected during nine alternative sampling scenarios, including six shorter sampling periods and three in which a fixed number of milk samples per cow were collected.
27600967	9	109	theme	Test	1935:1938	arg1	herd					1921:1924	the herd	1917:1924	the herd (NZ Herd Test Standard 8100:2015)	1917:1958	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	9	109	theme	Test	1935:1938	arg1	8100:2015					1949:1957	NZ Herd Test Standard 8100:2015	1927:1957	NZ Herd Test Standard 8100:2015	1927:1957	Based on the results of this study New Zealand have adopted a split protocol for herd testing based on the average milking frequency for the herd (NZ Herd Test Standard 8100:2015).
27600967	4	110	theme	Standard	705:712	arg1	protocol					714:721	The Gold Standard protocol	696:721	The Gold Standard protocol	696:721	The Gold Standard protocol was applied on five commercial dairy farms with AMS, between December 2011 and February 2013.
27600967	2	111	theme	indoor	364:369	arg1	cows					375:378	herd testing indoor fed cows	351:378	herd testing indoor fed cows	351:378	Therefore, milk recording intervals used for herd testing indoor fed cows may not be suitable for cows on pasture based farms.
27600967	3	112	theme	recording	621:629	arg1	interval					631:638	the optimum milk recording interval	604:638	the optimum milk recording interval	604:638	We hypothesised that accurate standardised 24 h estimates could be determined for AMS herds with milk recording intervals of less than the Gold Standard (48 hs), but that the optimum milk recording interval would depend on the herd average for milking frequency.
27600967	8	113	from	variations	1612:1621	arg1	composition					1631:1641	milk composition	1626:1641	milk composition	1626:1641	Further research is required to determine the effects of diurnal variations in milk composition on standardised 24 h estimates for milk volume and components, before a protocol based on a fixed number of samples could be considered.
27600967	6	114	theme	based	1343:1347	arg1	farms					1353:1357	pasture based AMS farms	1335:1357	pasture based AMS farms	1335:1357	Results infer a 48 h milk recording protocol is unnecessarily long for collecting accurate estimates during milk recording on pasture based AMS farms.
25640843	7	0	theme	microbiota	1598:1607	arg1	balance					1576:1582	the balance	1572:1582	the balance of intestinal microbiota	1572:1607	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	6	1	theme	dietary	1176:1182	arg1	supplementation					1193:1207	dietary β-glucan supplementation	1176:1207	dietary β-glucan supplementation	1176:1207	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	7	2	theme	Dietary	1397:1403	arg1	supplementation					1414:1428	Dietary β-glucan supplementation	1397:1428	Dietary β-glucan supplementation	1397:1428	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	1	3	theme	host	242:245	arg1	microbiota					258:267	the host intestinal microbiota	238:267	the host intestinal microbiota	238:267	β-glucan is a prebiotic well known for its beneficial outcomes on sea cucumber health through modifying the host intestinal microbiota.
25640843	7	4	theme	signaling	1525:1533	arg1	pathway					1535:1541	NF-κB signaling pathway	1519:1541	NF-κB signaling pathway	1519:1541	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	0	5	from	microbiota	11:20	arg1	response					88:95	sea cucumber (Apostichopus japonicus) response	50:95	sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation	50:131	Intestinal microbiota and immune related genes in sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation.
25640843	7	6	theme	intestinal	1587:1596	arg1	microbiota					1598:1607	intestinal microbiota	1587:1607	intestinal microbiota	1587:1607	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	2	7	theme	microbes	378:385	arg1	characterization					358:373	characterization	358:373	characterization	358:373	High-throughput sequencing techniques provide an opportunity for the identification and characterization of microbes.
25640843	2	7	theme	microbes	378:385	arg1	identification					339:352	identification	339:352	identification	339:352	High-throughput sequencing techniques provide an opportunity for the identification and characterization of microbes.
25640843	1	8	theme	intestinal	247:256	arg1	microbiota					258:267	the host intestinal microbiota	238:267	the host intestinal microbiota	238:267	β-glucan is a prebiotic well known for its beneficial outcomes on sea cucumber health through modifying the host intestinal microbiota.
25640843	6	9	theme	OTUs	1365:1368	arg1	composition					1324:1334	microbial community composition	1304:1334	microbial community composition	1304:1334	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	6	9	theme	OTUs	1365:1368	arg1	roles					1352:1356	topological roles	1340:1356	topological roles	1340:1356	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	7	10	theme	cucumber	1496:1503	arg1	intestine					1479:1487	the intestine	1475:1487	the intestine of sea cucumber	1475:1503	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	5	11	theme	β-glucan	858:865	arg1	supplementation					867:881	Dietary β-glucan supplementation	850:881	Dietary β-glucan supplementation	850:881	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	6	12	from	OTUs	1365:1368	arg1	network					1388:1394	the ecological network	1373:1394	the ecological network	1373:1394	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	7	13	theme	β-glucan	1405:1412	arg1	supplementation					1414:1428	Dietary β-glucan supplementation	1397:1428	Dietary β-glucan supplementation	1397:1428	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	6	14	theme	topological	1340:1350	arg1	roles					1352:1356	topological roles	1340:1356	topological roles	1340:1356	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	7	15	theme	positive	1436:1443	arg1	impact					1445:1450	a positive impact	1434:1450	a positive impact on immune responses of the intestine of sea cucumber	1434:1503	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	0	16	theme	dietary	100:106	arg1	supplementation					117:131	dietary β-glucan supplementation	100:131	dietary β-glucan supplementation	100:131	Intestinal microbiota and immune related genes in sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation.
25640843	5	17	theme	family	917:922	arg1	Rhodobacteraceae					924:939	the family Rhodobacteraceae	913:939	the family Rhodobacteraceae of the Alphaproteobacteria class	913:972	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	2	18	theme	sequencing	286:295	arg1	techniques					297:306	High-throughput sequencing techniques	270:306	High-throughput sequencing techniques	270:306	High-throughput sequencing techniques provide an opportunity for the identification and characterization of microbes.
25640843	5	19	theme	class	1033:1037	arg1	Verrucomicrobiaceae					989:1007	the family Verrucomicrobiaceae	978:1007	the family Verrucomicrobiaceae of the Verrucomicrobiae class	978:1037	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	5	19	theme	class	1033:1037	arg1	proliferation					896:908	the proliferation	892:908	the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class	892:972	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	5	20	theme	Dietary	850:856	arg1	supplementation					867:881	Dietary β-glucan supplementation	850:881	Dietary β-glucan supplementation	850:881	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	0	21	from	genes	41:45	arg1	response					88:95	sea cucumber (Apostichopus japonicus) response	50:95	sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation	50:131	Intestinal microbiota and immune related genes in sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation.
25640843	6	22	theme	functional	1270:1279	arg1	groups					1281:1286	different microbial functional groups	1250:1286	different microbial functional groups	1250:1286	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	2	23	theme	High-throughput	270:284	arg1	techniques					297:306	High-throughput sequencing techniques	270:306	High-throughput sequencing techniques	270:306	High-throughput sequencing techniques provide an opportunity for the identification and characterization of microbes.
25640843	6	24	theme	network	1223:1229	arg1	interactions					1231:1242	the network interactions	1219:1242	the network interactions among different microbial functional groups	1219:1286	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	5	25	theme	Alphaproteobacteria	948:966	arg1	class					968:972	the Alphaproteobacteria class	944:972	the Alphaproteobacteria class	944:972	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	7	26	contain	has	1430:1432	arg1	supplementation					1414:1428	Dietary β-glucan supplementation	1397:1428	Dietary β-glucan supplementation	1397:1428	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	7	26	contain	has	1430:1432	arg2	impact					1445:1450	a positive impact	1434:1450	a positive impact on immune responses of the intestine of sea cucumber	1434:1503	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	7	27	theme	immune	1455:1460	arg1	responses					1462:1470	immune responses	1455:1470	immune responses	1455:1470	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	3	28	from	genes	516:520	arg1	cucumber					529:536	sea cucumber	525:536	sea cucumber fed with diet supplemented with or without β-glucan supplementation	525:604	In this study, we investigated the intestinal microbial community composition, interaction among species, and intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation.
25640843	6	29	theme	community	1314:1322	arg1	composition					1324:1334	microbial community composition	1304:1334	microbial community composition	1304:1334	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	6	30	dep	composition	1324:1334	arg1	the					1300:1302	the	1300:1302	the	1300:1302	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	5	31	theme	class	968:972	arg1	Rhodobacteraceae					924:939	the family Rhodobacteraceae	913:939	the family Rhodobacteraceae of the Alphaproteobacteria class	913:972	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	0	32	theme	Intestinal	0:9	arg1	microbiota					11:20	Intestinal microbiota	0:20	Intestinal microbiota	0:20	Intestinal microbiota and immune related genes in sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation.
25640843	5	33	theme	Flavobacteriaceae	1088:1104	arg1	abundance					1064:1072	the relative abundance	1051:1072	the relative abundance of the family Flavobacteriaceae of Flavobacteria class	1051:1127	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	7	34	from	impact	1445:1450	arg1	responses					1462:1470	immune responses	1455:1470	immune responses	1455:1470	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	2	35	dep	identification	339:352	arg1	the					335:337	the	335:337	the	335:337	High-throughput sequencing techniques provide an opportunity for the identification and characterization of microbes.
25640843	1	36	theme	beneficial	177:186	arg1	outcomes					188:195	its beneficial outcomes	173:195	its beneficial outcomes on sea cucumber health	173:218	β-glucan is a prebiotic well known for its beneficial outcomes on sea cucumber health through modifying the host intestinal microbiota.
25640843	0	37	theme	immune	26:31	arg1	genes					41:45	immune related genes	26:45	immune related genes	26:45	Intestinal microbiota and immune related genes in sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation.
25640843	3	38	theme	β-glucan	581:588	arg1	supplementation					590:604	β-glucan supplementation	581:604	β-glucan supplementation	581:604	In this study, we investigated the intestinal microbial community composition, interaction among species, and intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation.
25640843	4	39	theme	control	668:674	arg1	group					676:680	the control group	664:680	the control group	664:680	The results show that the intestinal dominant classes in the control group are Flavobacteriia, Gammaproteobacteria, and Alphaproteobacteria, whereas Alphaproteobacteria, Flavobacteriia, and Verrucomicrobiae are enriched in the β-glucan group.
25640843	6	40	theme	microbial	1304:1312	arg1	composition					1324:1334	microbial community composition	1304:1334	microbial community composition	1304:1334	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	0	41	theme	β-glucan	108:115	arg1	supplementation					117:131	dietary β-glucan supplementation	100:131	dietary β-glucan supplementation	100:131	Intestinal microbiota and immune related genes in sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation.
25640843	0	42	dep	cucumber	54:61	arg1	japonicus					77:85	Apostichopus japonicus	64:85	Apostichopus japonicus	64:85	Intestinal microbiota and immune related genes in sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation.
25640843	3	43	theme	intestinal	498:507	arg1	genes					516:520	intestinal immune genes	498:520	intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation	498:604	In this study, we investigated the intestinal microbial community composition, interaction among species, and intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation.
25640843	3	44	theme	microbial	434:442	arg1	composition					454:464	the intestinal microbial community composition	419:464	the intestinal microbial community composition	419:464	In this study, we investigated the intestinal microbial community composition, interaction among species, and intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation.
25640843	5	45	theme	Verrucomicrobiae	1016:1031	arg1	class					1033:1037	the Verrucomicrobiae class	1012:1037	the Verrucomicrobiae class	1012:1037	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	5	46	theme	Rhodobacteraceae	924:939	arg1	Verrucomicrobiaceae					989:1007	the family Verrucomicrobiaceae	978:1007	the family Verrucomicrobiaceae of the Verrucomicrobiae class	978:1037	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	5	46	theme	Rhodobacteraceae	924:939	arg1	proliferation					896:908	the proliferation	892:908	the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class	892:972	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	1	47	theme	sea	200:202	arg1	cucumber					204:211	sea cucumber	200:211	sea cucumber health	200:218	β-glucan is a prebiotic well known for its beneficial outcomes on sea cucumber health through modifying the host intestinal microbiota.
25640843	0	48	theme	related	33:39	arg1	genes					41:45	immune related genes	26:45	immune related genes	26:45	Intestinal microbiota and immune related genes in sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation.
25640843	3	49	theme	community	444:452	arg1	composition					454:464	the intestinal microbial community composition	419:464	the intestinal microbial community composition	419:464	In this study, we investigated the intestinal microbial community composition, interaction among species, and intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation.
25640843	7	50	theme	sea	1492:1494	arg1	cucumber					1496:1503	sea cucumber	1492:1503	sea cucumber	1492:1503	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	6	51	from	roles	1352:1356	arg1	network					1388:1394	the ecological network	1373:1394	the ecological network	1373:1394	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	1	52	theme	cucumber	204:211	arg1	health					213:218	sea cucumber health	200:218	sea cucumber health	200:218	β-glucan is a prebiotic well known for its beneficial outcomes on sea cucumber health through modifying the host intestinal microbiota.
25640843	0	53	theme	sea	50:52	arg1	cucumber					54:61	sea cucumber	50:61	sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation	50:131	Intestinal microbiota and immune related genes in sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation.
25640843	4	54	from	classes	653:659	arg1	group					676:680	the control group	664:680	the control group	664:680	The results show that the intestinal dominant classes in the control group are Flavobacteriia, Gammaproteobacteria, and Alphaproteobacteria, whereas Alphaproteobacteria, Flavobacteriia, and Verrucomicrobiae are enriched in the β-glucan group.
25640843	6	55	theme	ecological	1377:1386	arg1	network					1388:1394	the ecological network	1373:1394	the ecological network	1373:1394	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	6	56	from	composition	1324:1334	arg1	network					1388:1394	the ecological network	1373:1394	the ecological network	1373:1394	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	3	57	theme	intestinal	423:432	arg1	composition					454:464	the intestinal microbial community composition	419:464	the intestinal microbial community composition	419:464	In this study, we investigated the intestinal microbial community composition, interaction among species, and intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation.
25640843	1	58	from	outcomes	188:195	arg1	health					213:218	sea cucumber health	200:218	sea cucumber health	200:218	β-glucan is a prebiotic well known for its beneficial outcomes on sea cucumber health through modifying the host intestinal microbiota.
25640843	3	59	theme	sea	525:527	arg1	cucumber					529:536	sea cucumber	525:536	sea cucumber fed with diet supplemented with or without β-glucan supplementation	525:604	In this study, we investigated the intestinal microbial community composition, interaction among species, and intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation.
25640843	7	60	theme	NF-κB	1519:1523	arg1	pathway					1535:1541	NF-κB signaling pathway	1519:1541	NF-κB signaling pathway	1519:1541	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	6	61	theme	network	1145:1151	arg1	analysis					1153:1160	The ecological network analysis	1130:1160	The ecological network analysis	1130:1160	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	3	62	theme	immune	509:514	arg1	genes					516:520	intestinal immune genes	498:520	intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation	498:604	In this study, we investigated the intestinal microbial community composition, interaction among species, and intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation.
25640843	5	63	theme	relative	1055:1062	arg1	abundance					1064:1072	the relative abundance	1051:1072	the relative abundance of the family Flavobacteriaceae of Flavobacteria class	1051:1127	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	4	64	theme	dominant	644:651	arg1	classes					653:659	the intestinal dominant classes	629:659	the intestinal dominant classes in the control group	629:680	The results show that the intestinal dominant classes in the control group are Flavobacteriia, Gammaproteobacteria, and Alphaproteobacteria, whereas Alphaproteobacteria, Flavobacteriia, and Verrucomicrobiae are enriched in the β-glucan group.
25640843	4	64	theme	dominant	644:651	arg1	Flavobacteriia					686:699	Flavobacteriia	686:699	Flavobacteriia	686:699	The results show that the intestinal dominant classes in the control group are Flavobacteriia, Gammaproteobacteria, and Alphaproteobacteria, whereas Alphaproteobacteria, Flavobacteriia, and Verrucomicrobiae are enriched in the β-glucan group.
25640843	4	65	theme	β-glucan	834:841	arg1	group					843:847	the β-glucan group	830:847	the β-glucan group	830:847	The results show that the intestinal dominant classes in the control group are Flavobacteriia, Gammaproteobacteria, and Alphaproteobacteria, whereas Alphaproteobacteria, Flavobacteriia, and Verrucomicrobiae are enriched in the β-glucan group.
25640843	5	66	theme	Flavobacteria	1109:1121	arg1	class					1123:1127	Flavobacteria class	1109:1127	Flavobacteria class	1109:1127	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	3	67	from	composition	454:464	arg1	cucumber					529:536	sea cucumber	525:536	sea cucumber fed with diet supplemented with or without β-glucan supplementation	525:604	In this study, we investigated the intestinal microbial community composition, interaction among species, and intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation.
25640843	6	68	theme	microbial	1260:1268	arg1	groups					1281:1286	different microbial functional groups	1250:1286	different microbial functional groups	1250:1286	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	7	69	theme	intestine	1479:1487	arg1	impact					1445:1450	a positive impact	1434:1450	a positive impact on immune responses of the intestine of sea cucumber	1434:1503	Dietary β-glucan supplementation has a positive impact on immune responses of the intestine of sea cucumber by activating NF-κB signaling pathway, probably through modulating the balance of intestinal microbiota.
25640843	4	70	theme	intestinal	633:642	arg1	classes					653:659	the intestinal dominant classes	629:659	the intestinal dominant classes in the control group	629:680	The results show that the intestinal dominant classes in the control group are Flavobacteriia, Gammaproteobacteria, and Alphaproteobacteria, whereas Alphaproteobacteria, Flavobacteriia, and Verrucomicrobiae are enriched in the β-glucan group.
25640843	4	70	theme	intestinal	633:642	arg1	Flavobacteriia					686:699	Flavobacteriia	686:699	Flavobacteriia	686:699	The results show that the intestinal dominant classes in the control group are Flavobacteriia, Gammaproteobacteria, and Alphaproteobacteria, whereas Alphaproteobacteria, Flavobacteriia, and Verrucomicrobiae are enriched in the β-glucan group.
25640843	5	71	theme	family	1081:1086	arg1	Flavobacteriaceae					1088:1104	the family Flavobacteriaceae	1077:1104	the family Flavobacteriaceae of Flavobacteria class	1077:1127	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	5	72	theme	class	1123:1127	arg1	Flavobacteriaceae					1088:1104	the family Flavobacteriaceae	1077:1104	the family Flavobacteriaceae of Flavobacteria class	1077:1127	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
25640843	6	73	theme	β-glucan	1184:1191	arg1	supplementation					1193:1207	dietary β-glucan supplementation	1176:1207	dietary β-glucan supplementation	1176:1207	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	6	74	theme	different	1250:1258	arg1	groups					1281:1286	different microbial functional groups	1250:1286	different microbial functional groups	1250:1286	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	6	75	from	network	1388:1394	arg1	composition					1324:1334	microbial community composition	1304:1334	microbial community composition	1304:1334	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	6	75	from	network	1388:1394	arg1	roles					1352:1356	topological roles	1340:1356	topological roles	1340:1356	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	6	76	theme	ecological	1134:1143	arg1	analysis					1153:1160	The ecological network analysis	1130:1160	The ecological network analysis	1130:1160	The ecological network analysis suggests that dietary β-glucan supplementation can alter the network interactions among different microbial functional groups by changing the microbial community composition and topological roles of the OTUs in the ecological network.
25640843	0	77	theme	cucumber	54:61	arg1	response					88:95	sea cucumber (Apostichopus japonicus) response	50:95	sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation	50:131	Intestinal microbiota and immune related genes in sea cucumber (Apostichopus japonicus) response to dietary β-glucan supplementation.
25640843	4	78	dep	Flavobacteriia	686:699	arg1	whereas					748:754	whereas	748:754	whereas	748:754	The results show that the intestinal dominant classes in the control group are Flavobacteriia, Gammaproteobacteria, and Alphaproteobacteria, whereas Alphaproteobacteria, Flavobacteriia, and Verrucomicrobiae are enriched in the β-glucan group.
25640843	3	79	from	interaction	467:477	arg1	cucumber					529:536	sea cucumber	525:536	sea cucumber fed with diet supplemented with or without β-glucan supplementation	525:604	In this study, we investigated the intestinal microbial community composition, interaction among species, and intestinal immune genes in sea cucumber fed with diet supplemented with or without β-glucan supplementation.
25640843	5	80	theme	family	982:987	arg1	Verrucomicrobiaceae					989:1007	the family Verrucomicrobiaceae	978:1007	the family Verrucomicrobiaceae of the Verrucomicrobiae class	978:1037	Dietary β-glucan supplementation promoted the proliferation of the family Rhodobacteraceae of the Alphaproteobacteria class and the family Verrucomicrobiaceae of the Verrucomicrobiae class and reduced the relative abundance of the family Flavobacteriaceae of Flavobacteria class.
24356985	4	0	theme	type	658:661	arg1	collagen					665:672	type I collagen	658:672	type I collagen	658:672	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	4	1	theme	collagen	665:672	arg1	scaffold					646:653	a degradable biopolymer scaffold	622:653	a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK)	622:810	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	10	2	theme	biomechanical	1845:1857	arg1	properties					1859:1868	the biomechanical properties	1841:1868	the biomechanical properties of ESS in vitro	1841:1884	These values provide instruction for improvement of the biomechanical properties of ESS in vitro that may reduce clinical morbidity from graft loss.
24356985	3	3	theme	composition	476:486	arg1	modifications					455:467	modifications	455:467	modifications of the composition of ESS	455:493	To consider modifications of the composition of ESS to improve homology with native skin, biomechanical analyses of the current composition of ESS were performed.
24356985	6	4	theme	murine	1293:1298	arg1	autograft					1300:1308	murine autograft	1293:1308	murine autograft	1293:1308	These biomechanical end points were also used to evaluate ESS at six weeks after grafting to full-thickness skin wounds in athymic mice and compared to murine autograft or excised murine skin.
24356985	6	5	from	wounds	1254:1259	arg1	mice					1272:1275	athymic mice	1264:1275	athymic mice	1264:1275	These biomechanical end points were also used to evaluate ESS at six weeks after grafting to full-thickness skin wounds in athymic mice and compared to murine autograft or excised murine skin.
24356985	5	6	theme	hydrated	839:846	arg1	scaffold					879:886	the scaffold	875:886	the scaffold populated with hF dermal skin substitute (DSS)	875:933	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	6	theme	hydrated	839:846	arg1	CGS					869:871	the hydrated biopolymer scaffold (CGS)	835:872	the hydrated biopolymer scaffold (CGS)	835:872	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	4	7	theme	I	663:663	arg1	collagen					665:672	type I collagen	658:672	type I collagen	658:672	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	9	8	theme	ESS	1729:1731	arg1	properties					1715:1724	mechanical properties	1704:1724	mechanical properties of ESS	1704:1731	These results demonstrate rapid changes in mechanical properties of ESS after grafting that are comparable to murine autograft.
24356985	10	9	theme	clinical	1902:1909	arg1	morbidity					1911:1919	clinical morbidity	1902:1919	clinical morbidity from graft loss	1902:1935	These values provide instruction for improvement of the biomechanical properties of ESS in vitro that may reduce clinical morbidity from graft loss.
24356985	9	10	from	changes	1693:1699	arg1	properties					1715:1724	mechanical properties	1704:1724	mechanical properties of ESS	1704:1731	These results demonstrate rapid changes in mechanical properties of ESS after grafting that are comparable to murine autograft.
24356985	7	11	theme	p	1389:1389	arg1	differences					1376:1386	statistically significant differences	1350:1386	statistically significant differences (p <0.05) between ESS in vitro and after grafting for all four structural properties	1350:1471	The data showed statistically significant differences (p <0.05) between ESS in vitro and after grafting for all four structural properties.
24356985	7	11	theme	p	1389:1389	arg1	<0.05					1391:1395	p <0.05	1389:1395	p <0.05	1389:1395	The data showed statistically significant differences (p <0.05) between ESS in vitro and after grafting for all four structural properties.
24356985	0	12	theme	full-thickness	90:103	arg1	wounds					105:110	full-thickness wounds	90:110	full-thickness wounds	90:110	Development of the mechanical properties of engineered skin substitutes after grafting to full-thickness wounds.
24356985	5	13	theme	hF	903:904	arg1	DSS					930:932	DSS	930:932	DSS	930:932	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	13	theme	hF	903:904	arg1	skin					913:916	hF dermal skin substitute	903:927	hF dermal skin substitute (DSS)	903:933	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	10	14	theme	graft	1926:1930	arg1	loss					1932:1935	graft loss	1926:1935	graft loss	1926:1935	These values provide instruction for improvement of the biomechanical properties of ESS in vitro that may reduce clinical morbidity from graft loss.
24356985	3	15	theme	native	520:525	arg1	skin					527:530	native skin	520:530	native skin	520:530	To consider modifications of the composition of ESS to improve homology with native skin, biomechanical analyses of the current composition of ESS were performed.
24356985	8	16	theme	maximum	1547:1553	arg1	extension					1555:1563	maximum extension	1547:1563	maximum extension at failure	1547:1574	Grafted ESS differed statistically from murine autograft with respect to maximum extension at failure, and from intact murine skin with respect to linear stiffness and maximum extension.
24356985	4	17	theme	chondroitin-sulfate	678:696	arg1	scaffold					646:653	a degradable biopolymer scaffold	622:653	a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK)	622:810	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	8	18	theme	maximum	1642:1648	arg1	extension					1650:1658	maximum extension	1642:1658	maximum extension	1642:1658	Grafted ESS differed statistically from murine autograft with respect to maximum extension at failure, and from intact murine skin with respect to linear stiffness and maximum extension.
24356985	1	19	dep	skin	124:127	arg1	substitutes					129:139	substitutes	129:139	substitutes	129:139	Engineered skin substitutes (ESSs) have been reported to close full-thickness burn wounds but are subject to loss from mechanical shear due to their deficiencies in tensile strength and elasticity.
24356985	5	20	theme	current	820:826	arg1	study					828:832	the current study	816:832	the current study	816:832	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	4	21	theme	degradable	624:633	arg1	scaffold					646:653	a degradable biopolymer scaffold	622:653	a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK)	622:810	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	5	22	theme	biopolymer	848:857	arg1	scaffold					879:886	the scaffold	875:886	the scaffold populated with hF dermal skin substitute (DSS)	875:933	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	22	theme	biopolymer	848:857	arg1	CGS					869:871	the hydrated biopolymer scaffold (CGS)	835:872	the hydrated biopolymer scaffold (CGS)	835:872	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	23	from	failure	1072:1078	arg1	energy					1090:1095	energy	1090:1095	energy absorbed up to the point of failure (N-mm)	1090:1138	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	23	from	failure	1072:1078	arg1	extension					1059:1067	maximum extension	1051:1067	maximum extension at failure (mm)	1051:1083	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	23	from	failure	1072:1078	arg1	stiffness					995:1003	linear stiffness	988:1003	linear stiffness (N/mm)	988:1010	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	23	from	failure	1072:1078	arg1	N/mm					1006:1009	N/mm	1006:1009	N/mm	1006:1009	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	23	from	failure	1072:1078	arg1	load					1030:1033	ultimate tensile load	1013:1033	ultimate tensile load at failure (N)	1013:1048	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	24	dep	point	1116:1120	arg1	up					1106:1107	up	1106:1107	up	1106:1107	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	4	25	theme	cultured	740:747	arg1	hF					775:776	hF	775:776	hF	775:776	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	4	25	theme	cultured	740:747	arg1	fibroblasts					762:772	cultured human dermal fibroblasts	740:772	cultured human dermal fibroblasts (hF)	740:777	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	2	26	theme	native	380:385	arg1	skin					387:390	native skin	380:390	native skin	380:390	Hypothetically, if the mechanical properties of ESS matched those of native skin, losses due to shear or fracture could be reduced.
24356985	5	27	theme	ultimate	1013:1020	arg1	load					1030:1033	ultimate tensile load	1013:1033	ultimate tensile load at failure (N)	1013:1048	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	1	28	from	deficiencies	262:273	arg1	elasticity					299:308	elasticity	299:308	elasticity	299:308	Engineered skin substitutes (ESSs) have been reported to close full-thickness burn wounds but are subject to loss from mechanical shear due to their deficiencies in tensile strength and elasticity.
24356985	1	28	from	deficiencies	262:273	arg1	strength					286:293	tensile strength	278:293	tensile strength	278:293	Engineered skin substitutes (ESSs) have been reported to close full-thickness burn wounds but are subject to loss from mechanical shear due to their deficiencies in tensile strength and elasticity.
24356985	9	29	theme	mechanical	1704:1713	arg1	properties					1715:1724	mechanical properties	1704:1724	mechanical properties of ESS	1704:1731	These results demonstrate rapid changes in mechanical properties of ESS after grafting that are comparable to murine autograft.
24356985	3	30	theme	biomechanical	533:545	arg1	analyses					547:554	biomechanical analyses	533:554	biomechanical analyses of the current composition of ESS	533:588	To consider modifications of the composition of ESS to improve homology with native skin, biomechanical analyses of the current composition of ESS were performed.
24356985	6	31	theme	biomechanical	1147:1159	arg1	points					1165:1170	These biomechanical end points	1141:1170	These biomechanical end points	1141:1170	These biomechanical end points were also used to evaluate ESS at six weeks after grafting to full-thickness skin wounds in athymic mice and compared to murine autograft or excised murine skin.
24356985	1	32	theme	full-thickness	176:189	arg1	wounds					196:201	close full-thickness burn wounds	170:201	close full-thickness burn wounds	170:201	Engineered skin substitutes (ESSs) have been reported to close full-thickness burn wounds but are subject to loss from mechanical shear due to their deficiencies in tensile strength and elasticity.
24356985	5	33	theme	maximum	1051:1057	arg1	extension					1059:1067	maximum extension	1051:1067	maximum extension at failure (mm)	1051:1083	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	10	34	theme	ESS	1873:1875	arg1	properties					1859:1868	the biomechanical properties	1841:1868	the biomechanical properties of ESS in vitro	1841:1884	These values provide instruction for improvement of the biomechanical properties of ESS in vitro that may reduce clinical morbidity from graft loss.
24356985	10	35	from	loss	1932:1935	arg1	morbidity					1911:1919	clinical morbidity	1902:1919	clinical morbidity from graft loss	1902:1935	These values provide instruction for improvement of the biomechanical properties of ESS in vitro that may reduce clinical morbidity from graft loss.
24356985	1	36	theme	burn	191:194	arg1	wounds					196:201	close full-thickness burn wounds	170:201	close full-thickness burn wounds	170:201	Engineered skin substitutes (ESSs) have been reported to close full-thickness burn wounds but are subject to loss from mechanical shear due to their deficiencies in tensile strength and elasticity.
24356985	0	37	theme	mechanical	19:28	arg1	properties					30:39	the mechanical properties	15:39	the mechanical properties of engineered skin	15:58	Development of the mechanical properties of engineered skin substitutes after grafting to full-thickness wounds.
24356985	9	38	theme	murine	1771:1776	arg1	autograft					1778:1786	murine autograft	1771:1786	murine autograft	1771:1786	These results demonstrate rapid changes in mechanical properties of ESS after grafting that are comparable to murine autograft.
24356985	1	39	theme	tensile	278:284	arg1	strength					286:293	tensile strength	278:293	tensile strength	278:293	Engineered skin substitutes (ESSs) have been reported to close full-thickness burn wounds but are subject to loss from mechanical shear due to their deficiencies in tensile strength and elasticity.
24356985	4	40	theme	human	749:753	arg1	hF					775:776	hF	775:776	hF	775:776	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	4	40	theme	human	749:753	arg1	fibroblasts					762:772	cultured human dermal fibroblasts	740:772	cultured human dermal fibroblasts (hF)	740:777	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	5	41	from	failure	1038:1044	arg1	energy					1090:1095	energy	1090:1095	energy absorbed up to the point of failure (N-mm)	1090:1138	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	41	from	failure	1038:1044	arg1	extension					1059:1067	maximum extension	1051:1067	maximum extension at failure (mm)	1051:1083	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	41	from	failure	1038:1044	arg1	stiffness					995:1003	linear stiffness	988:1003	linear stiffness (N/mm)	988:1010	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	41	from	failure	1038:1044	arg1	N/mm					1006:1009	N/mm	1006:1009	N/mm	1006:1009	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	41	from	failure	1038:1044	arg1	load					1030:1033	ultimate tensile load	1013:1033	ultimate tensile load at failure (N)	1013:1048	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	1	42	theme	Engineered	113:122	arg1	skin					124:127	Engineered skin substitutes	113:139	Engineered skin substitutes (ESSs)	113:146	Engineered skin substitutes (ESSs) have been reported to close full-thickness burn wounds but are subject to loss from mechanical shear due to their deficiencies in tensile strength and elasticity.
24356985	1	42	theme	Engineered	113:122	arg1	ESSs					142:145	ESSs	142:145	ESSs	142:145	Engineered skin substitutes (ESSs) have been reported to close full-thickness burn wounds but are subject to loss from mechanical shear due to their deficiencies in tensile strength and elasticity.
24356985	2	43	theme	due	400:402	arg1	losses					393:398	losses	393:398	losses due to shear or fracture	393:423	Hypothetically, if the mechanical properties of ESS matched those of native skin, losses due to shear or fracture could be reduced.
24356985	7	44	theme	structural	1451:1460	arg1	properties					1462:1471	all four structural properties	1442:1471	all four structural properties	1442:1471	The data showed statistically significant differences (p <0.05) between ESS in vitro and after grafting for all four structural properties.
24356985	1	45	from	shear	243:247	arg1	loss					222:225	loss	222:225	loss from mechanical shear due to their deficiencies in tensile strength and elasticity	222:308	Engineered skin substitutes (ESSs) have been reported to close full-thickness burn wounds but are subject to loss from mechanical shear due to their deficiencies in tensile strength and elasticity.
24356985	5	46	theme	linear	988:993	arg1	N/mm					1006:1009	N/mm	1006:1009	N/mm	1006:1009	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	46	theme	linear	988:993	arg1	stiffness					995:1003	linear stiffness	988:1003	linear stiffness (N/mm)	988:1010	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	3	47	theme	current	563:569	arg1	composition					571:581	the current composition	559:581	the current composition of ESS	559:588	To consider modifications of the composition of ESS to improve homology with native skin, biomechanical analyses of the current composition of ESS were performed.
24356985	4	48	theme	biopolymer	635:644	arg1	scaffold					646:653	a degradable biopolymer scaffold	622:653	a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK)	622:810	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	0	49	theme	properties	30:39	arg1	Development					0:10	Development	0:10	Development of the mechanical properties of engineered skin	0:58	Development of the mechanical properties of engineered skin substitutes after grafting to full-thickness wounds.
24356985	3	50	with	homology	506:513	arg1	skin					527:530	native skin	520:530	native skin	520:530	To consider modifications of the composition of ESS to improve homology with native skin, biomechanical analyses of the current composition of ESS were performed.
24356985	3	51	theme	composition	571:581	arg1	analyses					547:554	biomechanical analyses	533:554	biomechanical analyses of the current composition of ESS	533:588	To consider modifications of the composition of ESS to improve homology with native skin, biomechanical analyses of the current composition of ESS were performed.
24356985	8	52	theme	intact	1586:1591	arg1	skin					1600:1603	intact murine skin	1586:1603	intact murine skin with respect to linear stiffness and maximum extension	1586:1658	Grafted ESS differed statistically from murine autograft with respect to maximum extension at failure, and from intact murine skin with respect to linear stiffness and maximum extension.
24356985	0	53	theme	skin	55:58	arg1	properties					30:39	the mechanical properties	15:39	the mechanical properties of engineered skin	15:58	Development of the mechanical properties of engineered skin substitutes after grafting to full-thickness wounds.
24356985	6	54	theme	end	1161:1163	arg1	points					1165:1170	These biomechanical end points	1141:1170	These biomechanical end points	1141:1170	These biomechanical end points were also used to evaluate ESS at six weeks after grafting to full-thickness skin wounds in athymic mice and compared to murine autograft or excised murine skin.
24356985	7	55	theme	significant	1364:1374	arg1	differences					1376:1386	statistically significant differences	1350:1386	statistically significant differences (p <0.05) between ESS in vitro and after grafting for all four structural properties	1350:1471	The data showed statistically significant differences (p <0.05) between ESS in vitro and after grafting for all four structural properties.
24356985	7	55	theme	significant	1364:1374	arg1	<0.05					1391:1395	p <0.05	1389:1395	p <0.05	1389:1395	The data showed statistically significant differences (p <0.05) between ESS in vitro and after grafting for all four structural properties.
24356985	9	56	theme	rapid	1687:1691	arg1	changes					1693:1699	rapid changes	1687:1699	rapid changes in mechanical properties of ESS after grafting that are comparable to murine autograft	1687:1786	These results demonstrate rapid changes in mechanical properties of ESS after grafting that are comparable to murine autograft.
24356985	2	57	theme	mechanical	334:343	arg1	properties					345:354	the mechanical properties	330:354	the mechanical properties of ESS	330:361	Hypothetically, if the mechanical properties of ESS matched those of native skin, losses due to shear or fracture could be reduced.
24356985	0	58	theme	engineered	44:53	arg1	skin					55:58	engineered skin	44:58	engineered skin	44:58	Development of the mechanical properties of engineered skin substitutes after grafting to full-thickness wounds.
24356985	6	59	theme	skin	1249:1252	arg1	wounds					1254:1259	full-thickness skin wounds	1234:1259	full-thickness skin wounds in athymic mice	1234:1275	These biomechanical end points were also used to evaluate ESS at six weeks after grafting to full-thickness skin wounds in athymic mice and compared to murine autograft or excised murine skin.
24356985	4	60	theme	epidermal	783:791	arg1	hK					808:809	hK	808:809	hK	808:809	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	4	60	theme	epidermal	783:791	arg1	keratinocytes					793:805	epidermal keratinocytes	783:805	epidermal keratinocytes (hK)	783:810	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	5	61	dep	skin	913:916	arg1	substitute					918:927	substitute	918:927	substitute	918:927	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	62	theme	failure	1125:1131	arg1	point					1116:1120	the point	1112:1120	the point of failure (N-mm)	1112:1138	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	6	63	used	used	1182:1185	arg2	points					1165:1170	These biomechanical end points	1141:1170	These biomechanical end points	1141:1170	These biomechanical end points were also used to evaluate ESS at six weeks after grafting to full-thickness skin wounds in athymic mice and compared to murine autograft or excised murine skin.
24356985	8	64	theme	murine	1593:1598	arg1	skin					1600:1603	intact murine skin	1586:1603	intact murine skin with respect to linear stiffness and maximum extension	1586:1658	Grafted ESS differed statistically from murine autograft with respect to maximum extension at failure, and from intact murine skin with respect to linear stiffness and maximum extension.
24356985	6	65	theme	murine	1321:1326	arg1	skin					1328:1331	excised murine skin	1313:1331	excised murine skin	1313:1331	These biomechanical end points were also used to evaluate ESS at six weeks after grafting to full-thickness skin wounds in athymic mice and compared to murine autograft or excised murine skin.
24356985	6	66	theme	athymic	1264:1270	arg1	mice					1272:1275	athymic mice	1264:1275	athymic mice	1264:1275	These biomechanical end points were also used to evaluate ESS at six weeks after grafting to full-thickness skin wounds in athymic mice and compared to murine autograft or excised murine skin.
24356985	5	67	theme	scaffold	859:866	arg1	scaffold					879:886	the scaffold	875:886	the scaffold populated with hF dermal skin substitute (DSS)	875:933	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	67	theme	scaffold	859:866	arg1	CGS					869:871	the hydrated biopolymer scaffold (CGS)	835:872	the hydrated biopolymer scaffold (CGS)	835:872	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	4	68	theme	dermal	755:760	arg1	hF					775:776	hF	775:776	hF	775:776	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	4	68	theme	dermal	755:760	arg1	fibroblasts					762:772	cultured human dermal fibroblasts	740:772	cultured human dermal fibroblasts (hF)	740:777	ESSs consist of a degradable biopolymer scaffold of type I collagen and chondroitin-sulfate (CGS) that is populated sequentially with cultured human dermal fibroblasts (hF) and epidermal keratinocytes (hK).
24356985	6	69	theme	excised	1313:1319	arg1	skin					1328:1331	excised murine skin	1313:1331	excised murine skin	1313:1331	These biomechanical end points were also used to evaluate ESS at six weeks after grafting to full-thickness skin wounds in athymic mice and compared to murine autograft or excised murine skin.
24356985	5	70	theme	dermal	906:911	arg1	DSS					930:932	DSS	930:932	DSS	930:932	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	5	70	theme	dermal	906:911	arg1	skin					913:916	hF dermal skin substitute	903:927	hF dermal skin substitute (DSS)	903:933	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	8	71	from	failure	1568:1574	arg1	extension					1555:1563	maximum extension	1547:1563	maximum extension at failure	1547:1574	Grafted ESS differed statistically from murine autograft with respect to maximum extension at failure, and from intact murine skin with respect to linear stiffness and maximum extension.
24356985	3	72	theme	ESS	491:493	arg1	composition					476:486	the composition	472:486	the composition of ESS	472:493	To consider modifications of the composition of ESS to improve homology with native skin, biomechanical analyses of the current composition of ESS were performed.
24356985	1	73	theme	close	170:174	arg1	wounds					196:201	close full-thickness burn wounds	170:201	close full-thickness burn wounds	170:201	Engineered skin substitutes (ESSs) have been reported to close full-thickness burn wounds but are subject to loss from mechanical shear due to their deficiencies in tensile strength and elasticity.
24356985	10	74	theme	properties	1859:1868	arg1	improvement					1826:1836	improvement	1826:1836	improvement of the biomechanical properties of ESS in vitro	1826:1884	These values provide instruction for improvement of the biomechanical properties of ESS in vitro that may reduce clinical morbidity from graft loss.
24356985	6	75	theme	full-thickness	1234:1247	arg1	wounds					1254:1259	full-thickness skin wounds	1234:1259	full-thickness skin wounds in athymic mice	1234:1275	These biomechanical end points were also used to evaluate ESS at six weeks after grafting to full-thickness skin wounds in athymic mice and compared to murine autograft or excised murine skin.
24356985	5	76	theme	tensile	1022:1028	arg1	load					1030:1033	ultimate tensile load	1013:1033	ultimate tensile load at failure (N)	1013:1048	In the current study, the hydrated biopolymer scaffold (CGS), the scaffold populated with hF dermal skin substitute (DSS), or the complete ESS were evaluated mechanically for linear stiffness (N/mm), ultimate tensile load at failure (N), maximum extension at failure (mm), and energy absorbed up to the point of failure (N-mm).
24356985	3	77	theme	ESS	586:588	arg1	composition					571:581	the current composition	559:581	the current composition of ESS	559:588	To consider modifications of the composition of ESS to improve homology with native skin, biomechanical analyses of the current composition of ESS were performed.
24356985	8	78	theme	linear	1621:1626	arg1	stiffness					1628:1636	linear stiffness	1621:1636	linear stiffness	1621:1636	Grafted ESS differed statistically from murine autograft with respect to maximum extension at failure, and from intact murine skin with respect to linear stiffness and maximum extension.
24356985	2	79	theme	ESS	359:361	arg1	properties					345:354	the mechanical properties	330:354	the mechanical properties of ESS	330:361	Hypothetically, if the mechanical properties of ESS matched those of native skin, losses due to shear or fracture could be reduced.
24356985	8	80	theme	murine	1514:1519	arg1	autograft					1521:1529	murine autograft	1514:1529	murine autograft with respect to maximum extension at failure	1514:1574	Grafted ESS differed statistically from murine autograft with respect to maximum extension at failure, and from intact murine skin with respect to linear stiffness and maximum extension.
24356985	1	81	theme	mechanical	232:241	arg1	shear					243:247	mechanical shear	232:247	mechanical shear	232:247	Engineered skin substitutes (ESSs) have been reported to close full-thickness burn wounds but are subject to loss from mechanical shear due to their deficiencies in tensile strength and elasticity.
25299249	2	0	theme	adenine	518:524	arg1	oxidation					526:534	adenine oxidation	518:534	adenine oxidation	518:534	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	3	1	theme	accumulation	738:749	arg1	potential					751:759	accumulation time and accumulation potential	716:759	potential	751:759	The analytical performance was assessed with respect to the supporting electrolyte and its pH, accumulation time and accumulation potential, and the fluid flow rates.
25299249	5	2	theme	high	1063:1066	arg1	sensitivity					1068:1078	high sensitivity	1063:1078	high sensitivity	1063:1078	The proposed sensor showed advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility.
25299249	0	3	theme	guanine	105:111	arg1	quantification					87:100	simultaneous quantification	74:100	simultaneous quantification of guanine and adenine	74:123	Metal-organic frameworks and β-cyclodextrin-based composite electrode for simultaneous quantification of guanine and adenine in a lab-on-valve manifold.
25299249	0	4	from	frameworks	14:23	arg1	manifold					143:150	manifold	143:150	manifold	143:150	Metal-organic frameworks and β-cyclodextrin-based composite electrode for simultaneous quantification of guanine and adenine in a lab-on-valve manifold.
25299249	2	5	theme	behavior	603:610	arg1	interaction					560:570	the π-π stacking interaction	543:570	the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD	543:618	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	2	6	used	used	364:367	arg2	electrode					350:358	The electrode	346:358	The electrode	346:358	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	1	7	theme	novel	169:173	arg1	electrode					195:203	a novel chemically modified electrode	167:203	a novel chemically modified electrode	167:203	In this work, a novel chemically modified electrode is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material.
25299249	5	8	theme	sensitivity	1068:1078	arg1	advantages					1049:1058	advantages	1049:1058	advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility	1049:1160	The proposed sensor showed advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility.
25299249	2	9	theme	stacking	551:558	arg1	interaction					560:570	the π-π stacking interaction	543:570	the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD	543:618	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	2	10	theme	inclusion	593:601	arg1	behavior					603:610	the inclusion behavior	589:610	the inclusion behavior of β-CD	589:618	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	6	11	theme	practical	1176:1184	arg1	application					1186:1196	the practical application	1172:1196	the practical application of the proposed sensor	1172:1219	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	0	12	theme	adenine	117:123	arg1	quantification					87:100	simultaneous quantification	74:100	simultaneous quantification of guanine and adenine	74:123	Metal-organic frameworks and β-cyclodextrin-based composite electrode for simultaneous quantification of guanine and adenine in a lab-on-valve manifold.
25299249	5	13	theme	good	1141:1144	arg1	reproducibility					1146:1160	good reproducibility	1141:1160	good reproducibility	1141:1160	The proposed sensor showed advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility.
25299249	5	14	theme	reproducibility	1146:1160	arg1	advantages					1049:1058	advantages	1049:1058	advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility	1049:1160	The proposed sensor showed advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility.
25299249	2	15	theme	adenine	415:421	arg1	determination					386:398	simultaneous determination	373:398	simultaneous determination of guanine and adenine	373:421	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	0	16	from	electrode	60:68	arg1	manifold					143:150	manifold	143:150	manifold	143:150	Metal-organic frameworks and β-cyclodextrin-based composite electrode for simultaneous quantification of guanine and adenine in a lab-on-valve manifold.
25299249	1	17	theme	modified	186:193	arg1	electrode					195:203	a novel chemically modified electrode	167:203	a novel chemically modified electrode	167:203	In this work, a novel chemically modified electrode is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material.
25299249	6	18	theme	guanine	1265:1271	arg1	determination					1248:1260	the determination	1244:1260	the determination of guanine and adenine in real samples with satisfactory results	1244:1325	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	3	19	theme	accumulation	716:727	arg1	time					729:732	accumulation time and accumulation potential	716:759	time	729:732	The analytical performance was assessed with respect to the supporting electrolyte and its pH, accumulation time and accumulation potential, and the fluid flow rates.
25299249	4	20	theme	×	988:988	arg1	-1					1003:1004	-1	1003:1004	-1	1003:1004	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	4	20	theme	×	988:988	arg1	L					1001:1001	2.8 × 10(-8) mol L	984:1001	2.8 × 10(-8) mol L(-1)	984:1005	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	2	21	theme	guanine	403:409	arg1	determination					386:398	simultaneous determination	373:398	simultaneous determination of guanine and adenine	373:421	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	0	22	theme	Metal-organic	0:12	arg1	frameworks					14:23	Metal-organic frameworks	0:23	Metal-organic frameworks	0:23	Metal-organic frameworks and β-cyclodextrin-based composite electrode for simultaneous quantification of guanine and adenine in a lab-on-valve manifold.
25299249	2	23	theme	β-CD	615:618	arg1	behavior					603:610	the inclusion behavior	589:610	the inclusion behavior of β-CD	589:618	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	2	23	theme	β-CD	615:618	arg1	BTC					579:581	Cu3(BTC)2	575:583	Cu3(BTC)2	575:583	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	4	24	theme	detection	923:931	arg1	S/N					941:943	S/N = 3	941:947	S/N = 3	941:947	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	4	24	theme	detection	923:931	arg1	limits					933:938	detection limits	923:938	detection limits (S/N = 3)	923:948	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	6	25	with	samples	1293:1299	arg1	results					1319:1325	satisfactory results	1306:1325	satisfactory results	1306:1325	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	0	26	theme	β-cyclodextrin-based	29:48	arg1	electrode					60:68	β-cyclodextrin-based composite electrode	29:68	β-cyclodextrin-based composite electrode	29:68	Metal-organic frameworks and β-cyclodextrin-based composite electrode for simultaneous quantification of guanine and adenine in a lab-on-valve manifold.
25299249	5	27	theme	enhanced	1117:1124	arg1	throughput					1126:1135	enhanced throughput	1117:1135	enhanced throughput	1117:1135	The proposed sensor showed advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility.
25299249	2	28	theme	injection	439:447	arg1	format					462:467	a sequential injection lab-on-valve format	426:467	a sequential injection lab-on-valve format	426:467	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	2	29	theme	lab-on-valve	449:460	arg1	format					462:467	a sequential injection lab-on-valve format	426:467	a sequential injection lab-on-valve format	426:467	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	1	30	theme	BTC	290:292	arg1	benzene-1,3,5-tricarboxylate					296:323	BTC = benzene-1,3,5-tricarboxylate	290:323	BTC = benzene-1,3,5-tricarboxylate	290:323	In this work, a novel chemically modified electrode is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material.
25299249	2	31	theme	Cu3	575:577	arg1	BTC					579:581	Cu3(BTC)2	575:583	Cu3(BTC)2	575:583	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	6	32	from	determination	1248:1260	arg1	samples					1293:1299	real samples	1288:1299	real samples with satisfactory results	1288:1325	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	5	33	theme	throughput	1126:1135	arg1	advantages					1049:1058	advantages	1049:1058	advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility	1049:1160	The proposed sensor showed advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility.
25299249	2	34	theme	sequential	428:437	arg1	injection					439:447	a sequential injection	426:447	a sequential injection lab-on-valve format	426:467	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	1	35	theme	=	294:294	arg1	benzene-1,3,5-tricarboxylate					296:323	BTC = benzene-1,3,5-tricarboxylate	290:323	BTC = benzene-1,3,5-tricarboxylate	290:323	In this work, a novel chemically modified electrode is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material.
25299249	4	36	theme	×	899:899	arg1	L					912:912	1.0 × 10(-5) mol L	895:912	1.0 × 10(-5) mol L(-1)	895:916	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	4	36	theme	×	899:899	arg1	-1					914:915	-1	914:915	-1	914:915	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	6	37	theme	adenine	1277:1283	arg1	determination					1248:1260	the determination	1244:1260	the determination of guanine and adenine in real samples with satisfactory results	1244:1325	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	4	38	theme	linear	814:819	arg1	ranges					833:838	linear calibration ranges	814:838	linear calibration ranges for both guanine and adenine	814:867	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	0	39	theme	composite	50:58	arg1	electrode					60:68	β-cyclodextrin-based composite electrode	29:68	β-cyclodextrin-based composite electrode	29:68	Metal-organic frameworks and β-cyclodextrin-based composite electrode for simultaneous quantification of guanine and adenine in a lab-on-valve manifold.
25299249	3	40	theme	flow	776:779	arg1	rates					781:785	the fluid flow rates	766:785	the fluid flow rates	766:785	The analytical performance was assessed with respect to the supporting electrolyte and its pH, accumulation time and accumulation potential, and the fluid flow rates.
25299249	6	41	from	guanine	1265:1271	arg1	samples					1293:1299	real samples	1288:1299	real samples with satisfactory results	1288:1325	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	4	42	theme	=	945:945	arg1	S/N					941:943	S/N = 3	941:947	S/N = 3	941:947	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	4	42	theme	=	945:945	arg1	limits					933:938	detection limits	923:938	detection limits (S/N = 3)	923:948	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	1	43	theme	metal-organic	229:241	arg1	frameworks					243:252	metal-organic frameworks	229:252	metal-organic frameworks	229:252	In this work, a novel chemically modified electrode is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material.
25299249	0	44	theme	simultaneous	74:85	arg1	quantification					87:100	simultaneous quantification	74:100	simultaneous quantification of guanine and adenine	74:123	Metal-organic frameworks and β-cyclodextrin-based composite electrode for simultaneous quantification of guanine and adenine in a lab-on-valve manifold.
25299249	6	45	theme	proposed	1205:1212	arg1	sensor					1214:1219	the proposed sensor	1201:1219	the proposed sensor	1201:1219	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	6	46	from	adenine	1277:1283	arg1	samples					1293:1299	real samples	1288:1299	real samples with satisfactory results	1288:1325	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	2	47	theme	sensitive	483:491	arg1	responses					493:501	sensitive responses	483:501	sensitive responses to guanine and adenine oxidation	483:534	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	1	48	theme	composite	326:334	arg1	material					336:343	β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material	258:343	β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material	258:343	In this work, a novel chemically modified electrode is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material.
25299249	4	49	theme	calibration	821:831	arg1	ranges					833:838	linear calibration ranges	814:838	linear calibration ranges for both guanine and adenine	814:867	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	5	50	theme	simple	1081:1086	arg1	protocol					1107:1114	simple sample preparation protocol	1081:1114	simple sample preparation protocol	1081:1114	The proposed sensor showed advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility.
25299249	3	51	theme	fluid	770:774	arg1	rates					781:785	the fluid flow rates	766:785	the fluid flow rates	766:785	The analytical performance was assessed with respect to the supporting electrolyte and its pH, accumulation time and accumulation potential, and the fluid flow rates.
25299249	3	52	theme	supporting	681:690	arg1	electrolyte					692:702	the supporting electrolyte	677:702	the supporting electrolyte	677:702	The analytical performance was assessed with respect to the supporting electrolyte and its pH, accumulation time and accumulation potential, and the fluid flow rates.
25299249	6	53	theme	satisfactory	1306:1317	arg1	results					1319:1325	satisfactory results	1306:1325	satisfactory results	1306:1325	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	6	54	from	samples	1293:1299	arg1	determination					1248:1260	the determination	1244:1260	the determination of guanine and adenine in real samples with satisfactory results	1244:1325	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	5	55	theme	sample	1088:1093	arg1	protocol					1107:1114	simple sample preparation protocol	1081:1114	simple sample preparation protocol	1081:1114	The proposed sensor showed advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility.
25299249	5	56	theme	proposed	1026:1033	arg1	sensor					1035:1040	The proposed sensor	1022:1040	The proposed sensor	1022:1040	The proposed sensor showed advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility.
25299249	2	57	theme	BTC	579:581	arg1	interaction					560:570	the π-π stacking interaction	543:570	the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD	543:618	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	1	58	dep	Cu3	274:276	arg1	2/β-CD					282:287	2/β-CD	282:287	2/β-CD	282:287	In this work, a novel chemically modified electrode is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material.
25299249	1	58	dep	Cu3	274:276	arg1	BTC					278:280	BTC	278:280	BTC	278:280	In this work, a novel chemically modified electrode is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material.
25299249	1	58	dep	Cu3	274:276	arg1	benzene-1,3,5-tricarboxylate					296:323	BTC = benzene-1,3,5-tricarboxylate	290:323	BTC = benzene-1,3,5-tricarboxylate	290:323	In this work, a novel chemically modified electrode is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material.
25299249	3	59	theme	analytical	625:634	arg1	performance					636:646	The analytical performance	621:646	The analytical performance	621:646	The analytical performance was assessed with respect to the supporting electrolyte and its pH, accumulation time and accumulation potential, and the fluid flow rates.
25299249	5	60	theme	preparation	1095:1105	arg1	protocol					1107:1114	simple sample preparation protocol	1081:1114	simple sample preparation protocol	1081:1114	The proposed sensor showed advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility.
25299249	6	61	theme	sensor	1214:1219	arg1	application					1186:1196	the practical application	1172:1196	the practical application of the proposed sensor	1172:1219	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	1	62	dep	constructed	208:218	arg1	based					220:224	based	220:224	is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material	205:343	In this work, a novel chemically modified electrode is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material.
25299249	5	63	theme	protocol	1107:1114	arg1	advantages					1049:1058	advantages	1049:1058	advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility	1049:1160	The proposed sensor showed advantages of high sensitivity, simple sample preparation protocol, enhanced throughput and good reproducibility.
25299249	1	64	theme	β-cyclodextrin	258:271	arg1	material					336:343	β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material	258:343	β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material	258:343	In this work, a novel chemically modified electrode is constructed based on metal-organic frameworks and β-cyclodextrin (Cu3(BTC)2/β-CD, BTC = benzene-1,3,5-tricarboxylate) composite material.
25299249	4	65	theme	mol	908:910	arg1	L					912:912	1.0 × 10(-5) mol L	895:912	1.0 × 10(-5) mol L(-1)	895:916	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	4	65	theme	mol	908:910	arg1	-1					914:915	-1	914:915	-1	914:915	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	4	66	theme	optimal	794:800	arg1	conditions					802:811	optimal conditions	794:811	optimal conditions	794:811	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	4	67	theme	mol	997:999	arg1	-1					1003:1004	-1	1003:1004	-1	1003:1004	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	4	67	theme	mol	997:999	arg1	L					1001:1001	2.8 × 10(-8) mol L	984:1001	2.8 × 10(-8) mol L(-1)	984:1005	Under optimal conditions, linear calibration ranges for both guanine and adenine were from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and detection limits (S/N = 3) were found to be 5.2 × 10(-8) and 2.8 × 10(-8) mol L(-1), respectively.
25299249	2	68	theme	simultaneous	373:384	arg1	determination					386:398	simultaneous determination	373:398	simultaneous determination of guanine and adenine	373:421	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
25299249	6	69	theme	real	1288:1291	arg1	samples					1293:1299	real samples	1288:1299	real samples with satisfactory results	1288:1325	Finally, the practical application of the proposed sensor has been performed for the determination of guanine and adenine in real samples with satisfactory results.
25299249	2	70	theme	π-π	547:549	arg1	interaction					560:570	the π-π stacking interaction	543:570	the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD	543:618	The electrode was used for simultaneous determination of guanine and adenine in a sequential injection lab-on-valve format and exhibited sensitive responses to guanine and adenine oxidation due to the π-π stacking interaction of Cu3(BTC)2 and the inclusion behavior of β-CD.
24590624	5	0	theme	groups	762:767	arg1	number					745:750	The number	741:750	The number of methyl groups in a CD ring	741:780	The number of methyl groups in a CD ring should be around 14 to get the highest solubility for the included guest molecules.
24590624	6	1	theme	isomers	895:901	arg1	distribution					879:890	the distribution	875:890	the distribution of isomers and related compounds	875:923	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	4	2	theme	derivatization	694:707	arg1	composition					645:655	the composition	641:655	the composition	641:655	The effects of the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups were studied.
24590624	4	2	theme	derivatization	694:707	arg1	influence					673:681	the influence	669:681	methylation, the composition, as well as the influence of further derivatization with ionic groups	628:725	The effects of the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups were studied.
24590624	4	2	theme	derivatization	694:707	arg1	methylation					628:638	methylation	628:638	methylation	628:638	The effects of the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups were studied.
24590624	3	3	theme	methylated	512:521	arg1	β-CDs					523:527	All the commercially available methylated β-CDs	481:527	All the commercially available methylated β-CDs	481:527	All the commercially available methylated β-CDs are mixtures of various isomers and homologues except trimethyl β-CD.
24590624	3	3	theme	methylated	512:521	arg1	mixtures					533:540	mixtures	533:540	mixtures of various isomers	533:559	All the commercially available methylated β-CDs are mixtures of various isomers and homologues except trimethyl β-CD.
24590624	2	4	theme	solubilizing	359:370	arg1	capability					372:381	solubilizing capability	359:381	solubilizing capability	359:381	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	6	5	theme	substitution	969:980	arg1	degree					959:964	constant degree	950:964	constant degree of substitution	950:980	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	2	6	theme	magnetic	293:300	arg1	resonance					302:310	nuclear magnetic resonance	285:310	nuclear magnetic resonance spectroscopy	285:323	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	6	7	theme	succinyl	1013:1020	arg1	moieties					1022:1029	amino and succinyl moieties	1003:1029	moieties	1022:1029	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	4	8	with	derivatization	694:707	arg1	groups					720:725	ionic groups	714:725	ionic groups	714:725	The effects of the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups were studied.
24590624	2	9	theme	nuclear	285:291	arg1	resonance					302:310	nuclear magnetic resonance	285:310	nuclear magnetic resonance spectroscopy	285:323	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	4	10	theme	further	686:692	arg1	derivatization					694:707	further derivatization	686:707	further derivatization with ionic groups	686:725	The effects of the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups were studied.
24590624	6	11	theme	moieties	1022:1029	arg1	introduction					987:998	the introduction	983:998	the introduction of amino and succinyl moieties on the CD ring	983:1044	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	6	12	theme	amino	1003:1007	arg1	moieties					1022:1029	amino and succinyl moieties	1003:1029	moieties	1022:1029	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	5	13	theme	included	840:847	arg1	molecules					855:863	the included guest molecules	836:863	the included guest molecules	836:863	The number of methyl groups in a CD ring should be around 14 to get the highest solubility for the included guest molecules.
24590624	1	14	theme	soluble	163:169	arg1	compounds					179:187	poorly soluble organic compounds	156:187	poorly soluble organic compounds	156:187	Methylated cyclodextrins (CDs) are effective solubilizers of poorly soluble organic compounds.
24590624	6	15	from	introduction	987:998	arg1	ring					1041:1044	the CD ring	1034:1044	the CD ring	1034:1044	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	3	16	theme	various	545:551	arg1	isomers					553:559	various isomers	545:559	various isomers	545:559	All the commercially available methylated β-CDs are mixtures of various isomers and homologues except trimethyl β-CD.
24590624	6	17	theme	ionic	1051:1055	arg1	interactions					1057:1068	ionic interactions	1051:1068	ionic interactions	1051:1068	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	1	18	theme	organic	171:177	arg1	compounds					179:187	poorly soluble organic compounds	156:187	poorly soluble organic compounds	156:187	Methylated cyclodextrins (CDs) are effective solubilizers of poorly soluble organic compounds.
24590624	3	19	theme	trimethyl	583:591	arg1	β-CD					593:596	trimethyl β-CD	583:596	trimethyl β-CD	583:596	All the commercially available methylated β-CDs are mixtures of various isomers and homologues except trimethyl β-CD.
24590624	1	20	theme	compounds	179:187	arg1	solubilizers					140:151	effective solubilizers	130:151	effective solubilizers of poorly soluble organic compounds	130:187	Methylated cyclodextrins (CDs) are effective solubilizers of poorly soluble organic compounds.
24590624	1	20	theme	compounds	179:187	arg1	cyclodextrins					106:118	Methylated cyclodextrins	95:118	Methylated cyclodextrins (CDs)	95:124	Methylated cyclodextrins (CDs) are effective solubilizers of poorly soluble organic compounds.
24590624	6	21	theme	constant	950:957	arg1	degree					959:964	constant degree	950:964	constant degree of substitution	950:980	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	6	22	theme	formation	1127:1135	arg1	interactions					1089:1100	the hydrophobic interactions	1073:1100	the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization	1073:1192	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	2	23	theme	model	392:396	arg1	furosemide					442:451	furosemide	442:451	furosemide	442:451	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	2	23	theme	model	392:396	arg1	tamoxifen					454:462	tamoxifen	454:462	tamoxifen	454:462	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	2	23	theme	model	392:396	arg1	cholesterol					416:426	cholesterol	416:426	cholesterol	416:426	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	2	23	theme	model	392:396	arg1	acids					435:439	fatty acids	429:439	fatty acids	429:439	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	2	23	theme	model	392:396	arg1	amiodarone					469:478	amiodarone	469:478	amiodarone	469:478	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	2	23	theme	model	392:396	arg1	compounds					398:406	model compounds	392:406	model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone	392:478	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	1	24	theme	Methylated	95:104	arg1	solubilizers					140:151	effective solubilizers	130:151	effective solubilizers of poorly soluble organic compounds	130:187	Methylated cyclodextrins (CDs) are effective solubilizers of poorly soluble organic compounds.
24590624	1	24	theme	Methylated	95:104	arg1	CDs					121:123	CDs	121:123	CDs	121:123	Methylated cyclodextrins (CDs) are effective solubilizers of poorly soluble organic compounds.
24590624	1	24	theme	Methylated	95:104	arg1	cyclodextrins					106:118	Methylated cyclodextrins	95:118	Methylated cyclodextrins (CDs)	95:124	Methylated cyclodextrins (CDs) are effective solubilizers of poorly soluble organic compounds.
24590624	6	25	theme	synergic	1160:1167	arg1	effect					1169:1174	synergic effect	1160:1174	synergic effect in solubilization	1160:1192	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	2	26	theme	resonance	302:310	arg1	spectroscopy					312:323	nuclear magnetic resonance spectroscopy	285:323	nuclear magnetic resonance spectroscopy	285:323	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	2	27	theme	fatty	429:433	arg1	acids					435:439	fatty acids	429:439	fatty acids	429:439	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	0	28	theme	number	39:44	arg1	role					31:34	the role	27:34	Methyl-beta-cyclodextrins: the role of number and types of substituents in solubilizing power.	0:93	Methyl-beta-cyclodextrins: the role of number and types of substituents in solubilizing power.
24590624	5	29	from	number	745:750	arg1	ring					777:780	a CD ring	772:780	a CD ring	772:780	The number of methyl groups in a CD ring should be around 14 to get the highest solubility for the included guest molecules.
24590624	2	30	theme	methylated	224:233	arg1	β-CDs					235:239	various methylated β-CDs	216:239	various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone	216:478	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	5	31	theme	guest	849:853	arg1	molecules					855:863	the included guest molecules	836:863	the included guest molecules	836:863	The number of methyl groups in a CD ring should be around 14 to get the highest solubility for the included guest molecules.
24590624	5	32	theme	highest	813:819	arg1	solubility					821:830	the highest solubility	809:830	the highest solubility for the included guest molecules	809:863	The number of methyl groups in a CD ring should be around 14 to get the highest solubility for the included guest molecules.
24590624	5	33	theme	CD	774:775	arg1	ring					777:780	a CD ring	772:780	a CD ring	772:780	The number of methyl groups in a CD ring should be around 14 to get the highest solubility for the included guest molecules.
24590624	2	34	theme	various	216:222	arg1	β-CDs					235:239	various methylated β-CDs	216:239	various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone	216:478	In this work, we compared various methylated β-CDs concerning their structure characterized by nuclear magnetic resonance spectroscopy, composition analyzed by HPLC and solubilizing capability by using model compounds such as cholesterol, fatty acids, furosemide, tamoxifen, and amiodarone.
24590624	6	35	theme	CD	1038:1039	arg1	ring					1041:1044	the CD ring	1034:1044	the CD ring	1034:1044	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	0	36	theme	types	50:54	arg1	role					31:34	the role	27:34	Methyl-beta-cyclodextrins: the role of number and types of substituents in solubilizing power.	0:93	Methyl-beta-cyclodextrins: the role of number and types of substituents in solubilizing power.
24590624	4	37	theme	ionic	714:718	arg1	groups					720:725	ionic groups	714:725	ionic groups	714:725	The effects of the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups were studied.
24590624	6	38	theme	compounds	915:923	arg1	distribution					879:890	the distribution	875:890	the distribution of isomers and related compounds	875:923	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	3	39	theme	available	502:510	arg1	β-CDs					523:527	All the commercially available methylated β-CDs	481:527	All the commercially available methylated β-CDs	481:527	All the commercially available methylated β-CDs are mixtures of various isomers and homologues except trimethyl β-CD.
24590624	3	39	theme	available	502:510	arg1	mixtures					533:540	mixtures	533:540	mixtures of various isomers	533:559	All the commercially available methylated β-CDs are mixtures of various isomers and homologues except trimethyl β-CD.
24590624	6	40	theme	related	907:913	arg1	compounds					915:923	related compounds	907:923	related compounds	907:923	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	6	41	theme	complex	1119:1125	arg1	formation					1127:1135	the inclusion complex formation	1105:1135	the inclusion complex formation	1105:1135	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	4	42	theme	degree	618:623	arg1	effects					603:609	The effects	599:609	The effects of the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups	599:725	The effects of the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups were studied.
24590624	3	43	theme	isomers	553:559	arg1	β-CDs					523:527	All the commercially available methylated β-CDs	481:527	All the commercially available methylated β-CDs	481:527	All the commercially available methylated β-CDs are mixtures of various isomers and homologues except trimethyl β-CD.
24590624	3	43	theme	isomers	553:559	arg1	homologues					565:574	homologues	565:574	homologues except trimethyl β-CD	565:596	All the commercially available methylated β-CDs are mixtures of various isomers and homologues except trimethyl β-CD.
24590624	3	43	theme	isomers	553:559	arg1	mixtures					533:540	mixtures	533:540	mixtures of various isomers	533:559	All the commercially available methylated β-CDs are mixtures of various isomers and homologues except trimethyl β-CD.
24590624	6	44	theme	hydrophobic	1077:1087	arg1	interactions					1089:1100	the hydrophobic interactions	1073:1100	the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization	1073:1192	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	0	45	theme	substituents	59:70	arg1	number					39:44	number	39:44	number	39:44	Methyl-beta-cyclodextrins: the role of number and types of substituents in solubilizing power.
24590624	0	45	theme	substituents	59:70	arg1	types					50:54	types	50:54	types	50:54	Methyl-beta-cyclodextrins: the role of number and types of substituents in solubilizing power.
24590624	6	46	contain	has	925:927	arg2	effect					940:945	hardly any effect	929:945	hardly any effect	929:945	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	6	46	contain	has	925:927	arg1	distribution					879:890	the distribution	875:890	the distribution of isomers and related compounds	875:923	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	1	47	theme	effective	130:138	arg1	solubilizers					140:151	effective solubilizers	130:151	effective solubilizers of poorly soluble organic compounds	130:187	Methylated cyclodextrins (CDs) are effective solubilizers of poorly soluble organic compounds.
24590624	1	47	theme	effective	130:138	arg1	cyclodextrins					106:118	Methylated cyclodextrins	95:118	Methylated cyclodextrins (CDs)	95:124	Methylated cyclodextrins (CDs) are effective solubilizers of poorly soluble organic compounds.
24590624	4	48	theme	methylation	628:638	arg1	degree					618:623	the degree	614:623	the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups	614:725	The effects of the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups were studied.
24590624	5	49	theme	methyl	755:760	arg1	groups					762:767	methyl groups	755:767	methyl groups	755:767	The number of methyl groups in a CD ring should be around 14 to get the highest solubility for the included guest molecules.
24590624	6	50	theme	inclusion	1109:1117	arg1	formation					1127:1135	the inclusion complex formation	1105:1135	the inclusion complex formation	1105:1135	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
24590624	0	51	dep	Methyl-beta-cyclodextrins	0:24	arg1	role					31:34	the role	27:34	Methyl-beta-cyclodextrins: the role of number and types of substituents in solubilizing power.	0:93	Methyl-beta-cyclodextrins: the role of number and types of substituents in solubilizing power.
24590624	4	52	theme	influence	673:681	arg1	degree					618:623	the degree	614:623	the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups	614:725	The effects of the degree of methylation, the composition, as well as the influence of further derivatization with ionic groups were studied.
24590624	6	53	from	effect	1169:1174	arg1	solubilization					1179:1192	solubilization	1179:1192	solubilization	1179:1192	Although the distribution of isomers and related compounds has hardly any effect at constant degree of substitution, the introduction of amino and succinyl moieties on the CD ring adds ionic interactions to the hydrophobic interactions of the inclusion complex formation, which might result in synergic effect in solubilization.
28425978	5	0	theme	human	752:756	arg1	milk					758:761	human milk	752:761	human milk	752:761	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	3	1	from	use	386:388	arg1	practice					402:409	clinical practice	393:409	clinical practice	393:409	Mid-infrared (MIR) spectroscopy is an evolving technique for analyzing human milk but needs validation before use in clinical practice.
28425978	7	2	used	used	1100:1103	arg2	spectroscopy					1080:1091	MIR spectroscopy	1076:1091	MIR spectroscopy	1076:1091	CONCLUSIONS This methodological paper provides evidence that MIR spectroscopy can be used to analyze macronutrient composition of human milk.
28425978	4	3	theme	term	486:489	arg1	gestation					491:499	term gestation	486:499	term gestation	486:499	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	4	4	theme	chromatography	672:685	arg1	assay					687:691	high-pressure liquid chromatography assay	651:691	high-pressure liquid chromatography assay for lactose	651:703	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	5	5	theme	MIR	714:716	arg1	analysis					718:725	MIR analysis	714:725	MIR analysis of the macronutrients in human milk	714:761	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	5	6	with	tests	804:808	arg1	coefficients					838:849	intraclass correlation coefficients	815:849	intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose	815:907	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	4	7	theme	laboratory	570:579	arg1	methods					581:587	standard laboratory methods	561:587	standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose	561:703	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	3	8	theme	human	347:351	arg1	milk					353:356	human milk	347:356	human milk	347:356	Mid-infrared (MIR) spectroscopy is an evolving technique for analyzing human milk but needs validation before use in clinical practice.
28425978	2	9	theme	infants	267:273	arg1	intake					249:254	the intake	245:254	the intake of preterm infants	245:273	Hence, the nutrient profile is only an estimate and can result in under- or over-estimation of the intake of preterm infants.
28425978	4	10	theme	standard	561:568	arg1	methods					581:587	standard laboratory methods	561:587	standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose	561:703	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	7	11	theme	MIR	1076:1078	arg1	spectroscopy					1080:1091	MIR spectroscopy	1076:1091	MIR spectroscopy	1076:1091	CONCLUSIONS This methodological paper provides evidence that MIR spectroscopy can be used to analyze macronutrient composition of human milk.
28425978	2	12	theme	preterm	259:265	arg1	infants					267:273	preterm infants	259:273	preterm infants	259:273	Hence, the nutrient profile is only an estimate and can result in under- or over-estimation of the intake of preterm infants.
28425978	7	13	theme	macronutrient	1116:1128	arg1	composition					1130:1140	macronutrient composition	1116:1140	macronutrient composition of human milk	1116:1154	CONCLUSIONS This methodological paper provides evidence that MIR spectroscopy can be used to analyze macronutrient composition of human milk.
28425978	1	14	theme	OBJECTIVE	82:90	arg1	milk					98:101	OBJECTIVE Human milk	82:101	OBJECTIVE Human milk	82:101	OBJECTIVE Human milk has considerable variation in its composition.
28425978	5	15	theme	laboratory	793:802	arg1	tests					804:808	standard laboratory tests	784:808	standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose	784:907	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	4	16	theme	MIR	537:539	arg1	spectroscopy					541:552	MIR spectroscopy	537:552	MIR spectroscopy	537:552	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	4	17	from	mothers	452:458	arg1	samples					436:442	Human milk samples	425:442	Human milk samples from 35 mothers delivering at 35 weeks to term gestation	425:499	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	5	18	theme	macronutrients	734:747	arg1	analysis					718:725	MIR analysis	714:725	MIR analysis of the macronutrients in human milk	714:761	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	1	19	theme	Human	92:96	arg1	milk					98:101	OBJECTIVE Human milk	82:101	OBJECTIVE Human milk	82:101	OBJECTIVE Human milk has considerable variation in its composition.
28425978	0	20	theme	spectroscopy	27:38	arg1	Validation					0:9	Validation	0:9	Validation of mid-infrared spectroscopy for macronutrient analysis of human milk	0:79	Validation of mid-infrared spectroscopy for macronutrient analysis of human milk.
28425978	5	21	theme	correlation	826:836	arg1	coefficients					838:849	intraclass correlation coefficients	815:849	intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose	815:907	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	0	22	theme	mid-infrared	14:25	arg1	spectroscopy					27:38	mid-infrared spectroscopy	14:38	mid-infrared spectroscopy	14:38	Validation of mid-infrared spectroscopy for macronutrient analysis of human milk.
28425978	4	23	theme	milk	431:434	arg1	samples					436:442	Human milk samples	425:442	Human milk samples from 35 mothers delivering at 35 weeks to term gestation	425:499	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	1	24	theme	considerable	107:118	arg1	variation					120:128	considerable variation	107:128	considerable variation	107:128	OBJECTIVE Human milk has considerable variation in its composition.
28425978	0	25	theme	macronutrient	44:56	arg1	analysis					58:65	macronutrient analysis	44:65	macronutrient analysis of human milk	44:79	Validation of mid-infrared spectroscopy for macronutrient analysis of human milk.
28425978	0	26	theme	human	70:74	arg1	milk					76:79	human milk	70:79	human milk	70:79	Validation of mid-infrared spectroscopy for macronutrient analysis of human milk.
28425978	4	27	theme	Human	425:429	arg1	samples					436:442	Human milk samples	425:442	Human milk samples from 35 mothers delivering at 35 weeks to term gestation	425:499	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	4	28	theme	Mojonnier	623:631	arg1	assay					633:637	Mojonnier assay	623:637	Mojonnier assay for fat	623:645	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	3	29	theme	clinical	393:400	arg1	practice					402:409	clinical practice	393:409	clinical practice	393:409	Mid-infrared (MIR) spectroscopy is an evolving technique for analyzing human milk but needs validation before use in clinical practice.
28425978	5	30	dep	RESULTS	706:712	arg1	correlated					763:772	correlated	763:772	correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose	763:907	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	7	31	theme	methodological	1032:1045	arg1	paper					1047:1051	This methodological paper	1027:1051	This methodological paper	1027:1051	CONCLUSIONS This methodological paper provides evidence that MIR spectroscopy can be used to analyze macronutrient composition of human milk.
28425978	7	32	theme	human	1145:1149	arg1	milk					1151:1154	human milk	1145:1154	human milk	1145:1154	CONCLUSIONS This methodological paper provides evidence that MIR spectroscopy can be used to analyze macronutrient composition of human milk.
28425978	5	33	theme	0.997	854:858	arg1	coefficients					838:849	intraclass correlation coefficients	815:849	intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose	815:907	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	1	34	contain	has	103:105	arg1	milk					98:101	OBJECTIVE Human milk	82:101	OBJECTIVE Human milk	82:101	OBJECTIVE Human milk has considerable variation in its composition.
28425978	1	34	contain	has	103:105	arg2	variation					120:128	considerable variation	107:128	considerable variation	107:128	OBJECTIVE Human milk has considerable variation in its composition.
28425978	3	35	theme	MIR	290:292	arg1	spectroscopy					295:306	Mid-infrared (MIR) spectroscopy	276:306	Mid-infrared (MIR) spectroscopy	276:306	Mid-infrared (MIR) spectroscopy is an evolving technique for analyzing human milk but needs validation before use in clinical practice.
28425978	3	35	theme	MIR	290:292	arg1	technique					323:331	an evolving technique	311:331	an evolving technique for analyzing human milk	311:356	Mid-infrared (MIR) spectroscopy is an evolving technique for analyzing human milk but needs validation before use in clinical practice.
28425978	2	36	theme	nutrient	161:168	arg1	estimate					189:196	estimate	189:196	estimate	189:196	Hence, the nutrient profile is only an estimate and can result in under- or over-estimation of the intake of preterm infants.
28425978	2	36	theme	nutrient	161:168	arg1	profile					170:176	the nutrient profile	157:176	the nutrient profile	157:176	Hence, the nutrient profile is only an estimate and can result in under- or over-estimation of the intake of preterm infants.
28425978	5	37	theme	standard	784:791	arg1	tests					804:808	standard laboratory tests	784:808	standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose	784:907	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	7	38	theme	milk	1151:1154	arg1	composition					1130:1140	macronutrient composition	1116:1140	macronutrient composition of human milk	1116:1154	CONCLUSIONS This methodological paper provides evidence that MIR spectroscopy can be used to analyze macronutrient composition of human milk.
28425978	3	39	theme	Mid-infrared	276:287	arg1	spectroscopy					295:306	Mid-infrared (MIR) spectroscopy	276:306	Mid-infrared (MIR) spectroscopy	276:306	Mid-infrared (MIR) spectroscopy is an evolving technique for analyzing human milk but needs validation before use in clinical practice.
28425978	3	39	theme	Mid-infrared	276:287	arg1	technique					323:331	an evolving technique	311:331	an evolving technique for analyzing human milk	311:356	Mid-infrared (MIR) spectroscopy is an evolving technique for analyzing human milk but needs validation before use in clinical practice.
28425978	5	40	from	milk	758:761	arg1	analysis					718:725	MIR analysis	714:725	MIR analysis of the macronutrients in human milk	714:761	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	2	41	theme	intake	249:254	arg1	under-					216:221	under-	216:221	under-	216:221	Hence, the nutrient profile is only an estimate and can result in under- or over-estimation of the intake of preterm infants.
28425978	2	41	theme	intake	249:254	arg1	over-estimation					226:240	over-estimation	226:240	over-estimation	226:240	Hence, the nutrient profile is only an estimate and can result in under- or over-estimation of the intake of preterm infants.
28425978	4	42	dep	DESIGN	418:423	arg1	analyzed					506:513	analyzed	506:513	were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose	501:703	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	5	43	from	macronutrients	734:747	arg1	milk					758:761	human milk	752:761	human milk	752:761	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	7	44	dep	CONCLUSIONS	1015:1025	arg1	provides					1053:1060	provides	1053:1060	provides evidence that MIR spectroscopy can be used to analyze macronutrient composition of human milk	1053:1154	CONCLUSIONS This methodological paper provides evidence that MIR spectroscopy can be used to analyze macronutrient composition of human milk.
28425978	0	45	theme	milk	76:79	arg1	analysis					58:65	macronutrient analysis	44:65	macronutrient analysis of human milk	44:79	Validation of mid-infrared spectroscopy for macronutrient analysis of human milk.
28425978	4	46	theme	STUDY	412:416	arg1	DESIGN					418:423	STUDY DESIGN	412:423	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.	412:704	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	4	47	theme	liquid	665:670	arg1	chromatography					672:685	high-pressure liquid chromatography	651:685	high-pressure liquid chromatography assay for lactose	651:703	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	5	48	from	analysis	718:725	arg1	milk					758:761	human milk	752:761	human milk	752:761	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	3	49	theme	evolving	314:321	arg1	spectroscopy					295:306	Mid-infrared (MIR) spectroscopy	276:306	Mid-infrared (MIR) spectroscopy	276:306	Mid-infrared (MIR) spectroscopy is an evolving technique for analyzing human milk but needs validation before use in clinical practice.
28425978	3	49	theme	evolving	314:321	arg1	technique					323:331	an evolving technique	311:331	an evolving technique for analyzing human milk	311:356	Mid-infrared (MIR) spectroscopy is an evolving technique for analyzing human milk but needs validation before use in clinical practice.
28425978	5	50	theme	intraclass	815:824	arg1	coefficients					838:849	intraclass correlation coefficients	815:849	intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose	815:907	RESULTS MIR analysis of the macronutrients in human milk correlated well with standard laboratory tests with intraclass correlation coefficients of 0.997 for fat, 0.839 for protein and 0.776 for lactose.
28425978	4	51	theme	high-pressure	651:663	arg1	chromatography					672:685	high-pressure liquid chromatography	651:685	high-pressure liquid chromatography assay for lactose	651:703	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
28425978	4	52	theme	Kjeldahl	595:602	arg1	assay					604:608	Kjeldahl assay	595:608	Kjeldahl assay for protein	595:620	STUDY DESIGN Human milk samples from 35 mothers delivering at 35 weeks to term gestation were analyzed for macronutrients by MIR spectroscopy and by standard laboratory methods using Kjeldahl assay for protein, Mojonnier assay for fat and high-pressure liquid chromatography assay for lactose.
26629816	0	0	theme	Faecalibacterium	80:95	arg1	Growth					26:31	Associated Growth	15:31	Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota	15:115	Levan Enhances Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota.
26629816	5	1	theme	microbiota	763:772	arg1	composition					742:752	The composition	738:752	The composition of fecal microbiota and profile of metabolites	738:799	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	8	2	dep	microcalorimetry	1334:1349	arg1	analyses					1403:1410	analyses	1403:1410	analyses	1403:1410	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	3	3	theme	defined	495:501	arg1	medium					503:508	phosphate-buffered defined medium	476:508	phosphate-buffered defined medium	476:508	Eleven fecal samples from healthy donors were incubated in phosphate-buffered defined medium with or without levan supplementation and varying presence of amino acids.
26629816	2	4	from	effect	272:277	arg1	dynamics					326:333	the growth dynamics	315:333	the growth dynamics	315:333	Here, we show the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota in vitro by using isothermal microcalorimetry.
26629816	2	4	from	effect	272:277	arg1	metabolism					339:348	metabolism	339:348	metabolism of fecal microbiota	339:368	Here, we show the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota in vitro by using isothermal microcalorimetry.
26629816	1	5	theme	several	237:243	arg1	studies					245:251	several studies	237:251	several studies	237:251	The role of dietary fiber in supporting healthy gut microbiota and overall well-being of the host has been revealed in several studies.
26629816	7	6	dep	acid-producing	1057:1070	arg1	Faecalibacterium					1078:1093	e.g. Faecalibacterium	1073:1093	e.g. Faecalibacterium	1073:1093	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa was observed in levan-supplemented media.
26629816	6	7	theme	metabolism	899:908	arg1	products					881:888	The main products	872:888	The main products of levan metabolism	872:908	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	6	7	theme	metabolism	899:908	arg1	acids					963:967	acetic, lactic, butyric, propionic and succinic acids	915:967	acetic, lactic, butyric, propionic and succinic acids	915:967	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	2	8	theme	growth	319:324	arg1	dynamics					326:333	the growth dynamics	315:333	the growth dynamics	315:333	Here, we show the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota in vitro by using isothermal microcalorimetry.
26629816	8	9	theme	dietary	1210:1216	arg1	fibers/prebiotics					1218:1234	other dietary fibers/prebiotics	1204:1234	other dietary fibers/prebiotics	1204:1234	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	6	10	theme	carbon	973:978	arg1	dioxide					980:986	carbon dioxide	973:986	carbon dioxide	973:986	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	0	11	theme	Fecal	100:104	arg1	Microbiota					106:115	Fecal Microbiota	100:115	Fecal Microbiota	100:115	Levan Enhances Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota.
26629816	6	12	theme	levan	893:897	arg1	metabolism					899:908	levan metabolism	893:908	levan metabolism	893:908	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	8	13	theme	fecal	1354:1358	arg1	samples					1360:1366	fecal samples	1354:1366	fecal samples linked to metabolite	1354:1387	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	4	14	theme	metabolites	691:701	arg1	concentrations					651:664	concentrations	651:664	concentrations of produced and consumed metabolites	651:701	The generation of heat, changes in pH and microbiota composition, concentrations of produced and consumed metabolites during the growth were determined.
26629816	4	14	theme	metabolites	691:701	arg1	changes					609:615	changes	609:615	changes in pH and microbiota composition	609:648	The generation of heat, changes in pH and microbiota composition, concentrations of produced and consumed metabolites during the growth were determined.
26629816	2	15	theme	polyfructan	294:304	arg1	levan					306:310	a bacterial polyfructan levan	282:310	a bacterial polyfructan levan	282:310	Here, we show the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota in vitro by using isothermal microcalorimetry.
26629816	4	16	theme	consumed	682:689	arg1	metabolites					691:701	produced and consumed metabolites	669:701	produced and consumed metabolites	669:701	The generation of heat, changes in pH and microbiota composition, concentrations of produced and consumed metabolites during the growth were determined.
26629816	7	17	located	observed	1105:1112	arg2	growth					1000:1005	Associated growth	989:1005	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa	989:1099	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa was observed in levan-supplemented media.
26629816	7	17	located	observed	1105:1112	arg1	media					1136:1140	levan-supplemented media	1117:1140	levan-supplemented media	1117:1140	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa was observed in levan-supplemented media.
26629816	8	18	theme	samples	1360:1366	arg1	microcalorimetry					1334:1349	isothermal microcalorimetry	1323:1349	isothermal microcalorimetry of fecal samples linked to metabolite	1323:1387	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	8	18	theme	samples	1360:1366	arg1	consortia					1393:1401	consortia	1393:1401	consortia	1393:1401	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	8	19	theme	levan	1180:1184	arg1	capacity					1168:1175	the capacity	1164:1175	the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota	1164:1295	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	1	20	theme	gut	166:168	arg1	microbiota					170:179	healthy gut microbiota	158:179	healthy gut microbiota	158:179	The role of dietary fiber in supporting healthy gut microbiota and overall well-being of the host has been revealed in several studies.
26629816	7	21	theme	levan-supplemented	1117:1134	arg1	media					1136:1140	levan-supplemented media	1117:1140	levan-supplemented media	1117:1140	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa was observed in levan-supplemented media.
26629816	6	22	theme	main	876:879	arg1	products					881:888	The main products	872:888	The main products of levan metabolism	872:908	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	6	22	theme	main	876:879	arg1	acids					963:967	acetic, lactic, butyric, propionic and succinic acids	915:967	acetic, lactic, butyric, propionic and succinic acids	915:967	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	5	23	theme	profile	778:784	arg1	composition					742:752	The composition	738:752	The composition of fecal microbiota and profile of metabolites	738:799	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	8	24	attach	linked	1368:1373	arg1	metabolite					1378:1387	metabolite	1378:1387	metabolite	1378:1387	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	8	24	attach	linked	1368:1373	arg2	samples					1360:1366	fecal samples	1354:1366	fecal samples linked to metabolite	1354:1387	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	3	25	theme	levan	526:530	arg1	supplementation					532:546	levan supplementation	526:546	levan supplementation	526:546	Eleven fecal samples from healthy donors were incubated in phosphate-buffered defined medium with or without levan supplementation and varying presence of amino acids.
26629816	2	26	theme	microbiota	359:368	arg1	dynamics					326:333	the growth dynamics	315:333	the growth dynamics	315:333	Here, we show the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota in vitro by using isothermal microcalorimetry.
26629816	2	26	theme	microbiota	359:368	arg1	metabolism					339:348	metabolism	339:348	metabolism of fecal microbiota	339:368	Here, we show the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota in vitro by using isothermal microcalorimetry.
26629816	4	27	from	changes	609:615	arg1	pH					620:621	pH	620:621	pH	620:621	The generation of heat, changes in pH and microbiota composition, concentrations of produced and consumed metabolites during the growth were determined.
26629816	4	27	from	changes	609:615	arg1	composition					638:648	microbiota composition	627:648	microbiota composition	627:648	The generation of heat, changes in pH and microbiota composition, concentrations of produced and consumed metabolites during the growth were determined.
26629816	2	28	theme	bacterial	284:292	arg1	levan					306:310	a bacterial polyfructan levan	282:310	a bacterial polyfructan levan	282:310	Here, we show the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota in vitro by using isothermal microcalorimetry.
26629816	2	29	theme	fecal	353:357	arg1	microbiota					359:368	fecal microbiota	353:368	fecal microbiota	353:368	Here, we show the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota in vitro by using isothermal microcalorimetry.
26629816	6	30	theme	succinic	954:961	arg1	products					881:888	The main products	872:888	The main products of levan metabolism	872:908	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	6	30	theme	succinic	954:961	arg1	acids					963:967	acetic, lactic, butyric, propionic and succinic acids	915:967	acetic, lactic, butyric, propionic and succinic acids	915:967	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	3	31	theme	amino	572:576	arg1	acids					578:582	amino acids	572:582	amino acids	572:582	Eleven fecal samples from healthy donors were incubated in phosphate-buffered defined medium with or without levan supplementation and varying presence of amino acids.
26629816	1	32	theme	overall	185:191	arg1	well-being					193:202	overall well-being	185:202	overall well-being	185:202	The role of dietary fiber in supporting healthy gut microbiota and overall well-being of the host has been revealed in several studies.
26629816	8	33	dep	composition	1252:1262	arg1	the					1248:1250	the	1248:1250	the	1248:1250	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	2	34	theme	isothermal	388:397	arg1	microcalorimetry					399:414	isothermal microcalorimetry	388:414	isothermal microcalorimetry	388:414	Here, we show the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota in vitro by using isothermal microcalorimetry.
26629816	0	35	theme	Associated	15:24	arg1	Growth					26:31	Associated Growth	15:31	Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota	15:115	Levan Enhances Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota.
26629816	6	36	theme	propionic	940:948	arg1	products					881:888	The main products	872:888	The main products of levan metabolism	872:908	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	6	36	theme	propionic	940:948	arg1	acids					963:967	acetic, lactic, butyric, propionic and succinic acids	915:967	acetic, lactic, butyric, propionic and succinic acids	915:967	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	7	37	theme	acid-producing	1057:1070	arg1	growth					1000:1005	Associated growth	989:1005	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa	989:1099	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa was observed in levan-supplemented media.
26629816	1	38	theme	healthy	158:164	arg1	microbiota					170:179	healthy gut microbiota	158:179	healthy gut microbiota	158:179	The role of dietary fiber in supporting healthy gut microbiota and overall well-being of the host has been revealed in several studies.
26629816	5	39	theme	levan	835:839	arg1	availability					858:869	substrate (levan and amino acids) availability	824:869	substrate (levan and amino acids) availability	824:869	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	5	39	theme	levan	835:839	arg1	acids					851:855	levan and amino acids	835:855	acids	851:855	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	0	40	theme	Bacteroides	36:46	arg1	Growth					26:31	Associated Growth	15:31	Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota	15:115	Levan Enhances Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota.
26629816	8	41	theme	colon	1280:1284	arg1	microbiota					1286:1295	colon microbiota	1280:1295	colon microbiota	1280:1295	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	5	42	dep	availability	858:869	arg1	response					812:819	response	812:819	response	812:819	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	4	43	theme	microbiota	627:636	arg1	composition					638:648	microbiota composition	627:648	microbiota composition	627:648	The generation of heat, changes in pH and microbiota composition, concentrations of produced and consumed metabolites during the growth were determined.
26629816	8	44	theme	fibers/prebiotics	1218:1234	arg1	capacity					1168:1175	the capacity	1164:1175	the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota	1164:1295	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	6	45	theme	butyric	931:937	arg1	products					881:888	The main products	872:888	The main products of levan metabolism	872:908	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	6	45	theme	butyric	931:937	arg1	acids					963:967	acetic, lactic, butyric, propionic and succinic acids	915:967	acetic, lactic, butyric, propionic and succinic acids	915:967	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	1	46	theme	host	211:214	arg1	microbiota					170:179	healthy gut microbiota	158:179	healthy gut microbiota	158:179	The role of dietary fiber in supporting healthy gut microbiota and overall well-being of the host has been revealed in several studies.
26629816	1	46	theme	host	211:214	arg1	well-being					193:202	overall well-being	185:202	overall well-being	185:202	The role of dietary fiber in supporting healthy gut microbiota and overall well-being of the host has been revealed in several studies.
26629816	0	47	theme	Escherichia	49:59	arg1	Growth					26:31	Associated Growth	15:31	Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota	15:115	Levan Enhances Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota.
26629816	7	48	dep	levan-degrading	1010:1024	arg1	Bacteroides					1032:1042	e.g. Bacteroides	1027:1042	e.g. Bacteroides	1027:1042	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa was observed in levan-supplemented media.
26629816	7	48	dep	levan-degrading	1010:1024	arg1	taxa					1096:1099	taxa	1096:1099	taxa	1096:1099	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa was observed in levan-supplemented media.
26629816	5	49	theme	fecal	757:761	arg1	microbiota					763:772	fecal microbiota	757:772	fecal microbiota	757:772	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	3	50	theme	acids	578:582	arg1	presence					560:567	presence	560:567	presence of amino acids	560:582	Eleven fecal samples from healthy donors were incubated in phosphate-buffered defined medium with or without levan supplementation and varying presence of amino acids.
26629816	8	51	theme	microbiota	1286:1295	arg1	function					1268:1275	function	1268:1275	function	1268:1275	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	8	51	theme	microbiota	1286:1295	arg1	composition					1252:1262	composition	1252:1262	composition	1252:1262	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	8	52	theme	other	1204:1208	arg1	fibers/prebiotics					1218:1234	other dietary fibers/prebiotics	1204:1234	other dietary fibers/prebiotics	1204:1234	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	6	53	theme	lactic	923:928	arg1	products					881:888	The main products	872:888	The main products of levan metabolism	872:908	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	6	53	theme	lactic	923:928	arg1	acids					963:967	acetic, lactic, butyric, propionic and succinic acids	915:967	acetic, lactic, butyric, propionic and succinic acids	915:967	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	6	54	theme	acetic	915:920	arg1	products					881:888	The main products	872:888	The main products of levan metabolism	872:908	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	6	54	theme	acetic	915:920	arg1	acids					963:967	acetic, lactic, butyric, propionic and succinic acids	915:967	acetic, lactic, butyric, propionic and succinic acids	915:967	The main products of levan metabolism were acetic, lactic, butyric, propionic and succinic acids and carbon dioxide.
26629816	3	55	theme	phosphate-buffered	476:493	arg1	medium					503:508	phosphate-buffered defined medium	476:508	phosphate-buffered defined medium	476:508	Eleven fecal samples from healthy donors were incubated in phosphate-buffered defined medium with or without levan supplementation and varying presence of amino acids.
26629816	7	56	theme	levan-degrading	1010:1024	arg1	growth					1000:1005	Associated growth	989:1005	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa	989:1099	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa was observed in levan-supplemented media.
26629816	0	57	from	Microbiota	106:115	arg1	Growth					26:31	Associated Growth	15:31	Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota	15:115	Levan Enhances Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota.
26629816	3	58	from	donors	451:456	arg1	samples					430:436	Eleven fecal samples	417:436	Eleven fecal samples from healthy donors	417:456	Eleven fecal samples from healthy donors were incubated in phosphate-buffered defined medium with or without levan supplementation and varying presence of amino acids.
26629816	2	59	theme	levan	306:310	arg1	effect					272:277	the effect	268:277	the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota	268:368	Here, we show the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota in vitro by using isothermal microcalorimetry.
26629816	4	60	theme	heat	603:606	arg1	generation					589:598	The generation	585:598	The generation of heat, changes in pH and microbiota composition, concentrations of produced and consumed metabolites during the growth	585:719	The generation of heat, changes in pH and microbiota composition, concentrations of produced and consumed metabolites during the growth were determined.
26629816	7	61	theme	Associated	989:998	arg1	growth					1000:1005	Associated growth	989:1005	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa	989:1099	Associated growth of levan-degrading (e.g. Bacteroides) and butyric acid-producing (e.g. Faecalibacterium) taxa was observed in levan-supplemented media.
26629816	1	62	theme	dietary	130:136	arg1	fiber					138:142	dietary fiber	130:142	dietary fiber	130:142	The role of dietary fiber in supporting healthy gut microbiota and overall well-being of the host has been revealed in several studies.
26629816	8	63	theme	isothermal	1323:1332	arg1	microcalorimetry					1334:1349	isothermal microcalorimetry	1323:1349	isothermal microcalorimetry of fecal samples linked to metabolite	1323:1387	The study shows that the capacity of levan and possibly also other dietary fibers/prebiotics to modulate the composition and function of colon microbiota can be predicted by using isothermal microcalorimetry of fecal samples linked to metabolite and consortia analyses.
26629816	0	64	theme	Streptococcus	62:74	arg1	Growth					26:31	Associated Growth	15:31	Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota	15:115	Levan Enhances Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota.
26629816	5	65	theme	amino	845:849	arg1	availability					858:869	substrate (levan and amino acids) availability	824:869	substrate (levan and amino acids) availability	824:869	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	5	65	theme	amino	845:849	arg1	acids					851:855	levan and amino acids	835:855	acids	851:855	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	3	66	theme	healthy	443:449	arg1	donors					451:456	healthy donors	443:456	healthy donors	443:456	Eleven fecal samples from healthy donors were incubated in phosphate-buffered defined medium with or without levan supplementation and varying presence of amino acids.
26629816	1	67	theme	fiber	138:142	arg1	role					122:125	The role	118:125	The role of dietary fiber in supporting healthy gut microbiota and overall well-being of the host	118:214	The role of dietary fiber in supporting healthy gut microbiota and overall well-being of the host has been revealed in several studies.
26629816	3	68	theme	fecal	424:428	arg1	samples					430:436	Eleven fecal samples	417:436	Eleven fecal samples from healthy donors	417:456	Eleven fecal samples from healthy donors were incubated in phosphate-buffered defined medium with or without levan supplementation and varying presence of amino acids.
26629816	5	69	theme	metabolites	789:799	arg1	profile					778:784	profile	778:784	profile of metabolites	778:799	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	5	69	theme	metabolites	789:799	arg1	microbiota					763:772	fecal microbiota	757:772	fecal microbiota	757:772	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	0	70	from	Growth	26:31	arg1	Microbiota					106:115	Fecal Microbiota	100:115	Fecal Microbiota	100:115	Levan Enhances Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota.
26629816	5	71	theme	substrate	824:832	arg1	availability					858:869	substrate (levan and amino acids) availability	824:869	substrate (levan and amino acids) availability	824:869	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	5	71	theme	substrate	824:832	arg1	acids					851:855	levan and amino acids	835:855	acids	851:855	The composition of fecal microbiota and profile of metabolites changed in response to substrate (levan and amino acids) availability.
26629816	4	72	theme	produced	669:676	arg1	metabolites					691:701	produced and consumed metabolites	669:701	produced and consumed metabolites	669:701	The generation of heat, changes in pH and microbiota composition, concentrations of produced and consumed metabolites during the growth were determined.
27659164	7	0	theme	other	1397:1401	arg1	ferrites					1416:1423	other mixed spinel ferrites	1397:1423	other mixed spinel ferrites	1397:1423	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	2	1	dep	0.8	529:531	arg1	to					526:527	to	526:527	to	526:527	Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation.
27659164	6	2	theme	AMF	1156:1158	arg1	exposure					1160:1167	AMF exposure	1156:1167	AMF exposure (i.e. heat-induced necrosis)	1156:1196	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	6	3	theme	different	1232:1240	arg1	values					1242:1247	different values	1232:1247	different values of x	1232:1252	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	7	4	theme	ferrites	1416:1423	arg1	characterization					1377:1392	further characterization	1369:1392	further characterization of other mixed spinel ferrites	1369:1423	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	7	5	theme	ground	1353:1358	arg1	work					1360:1363	the ground work	1349:1363	the ground work for further characterization of other mixed spinel ferrites	1349:1423	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	6	6	theme	tumor	1301:1305	arg1	cells					1307:1311	tumor cells	1301:1311	tumor cells	1301:1311	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	1	7	theme	external	205:212	arg1	AMF					242:244	AMF	242:244	AMF	242:244	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	1	7	theme	external	205:212	arg1	field					235:239	an external alternating magnetic field	202:239	an external alternating magnetic field (AMF)	202:245	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	6	8	theme	O4	1212:1213	arg1	composition					1215:1225	Fex Co1-x Fe2 O4 composition	1198:1225	Fex Co1-x Fe2 O4 composition with different values of x	1198:1252	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	0	9	theme	nanoensembles	110:122	arg1	contrast					69:76	hyperthermia and MRI contrast	48:76	hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles	48:122	Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles.
27659164	1	10	theme	water	392:396	arg1	relaxivity					398:407	enhanced water relaxivity	383:407	enhanced water relaxivity	383:407	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	1	11	theme	magnetic	226:233	arg1	AMF					242:244	AMF	242:244	AMF	242:244	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	1	11	theme	magnetic	226:233	arg1	field					235:239	an external alternating magnetic field	202:239	an external alternating magnetic field (AMF)	202:245	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	2	12	theme	chemical	549:556	arg1	co-precipitation					558:573	chemical co-precipitation	549:573	chemical co-precipitation	549:573	Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation.
27659164	6	13	theme	Co1-x	1202:1206	arg1	composition					1215:1225	Fex Co1-x Fe2 O4 composition	1198:1225	Fex Co1-x Fe2 O4 composition with different values of x	1198:1252	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	4	14	theme	water	717:721	arg1	relaxivity					736:745	MRI water (transverse) relaxivity	713:745	MRI water (transverse) relaxivity at 11.7 T	713:755	MRI water (transverse) relaxivity at 11.7 T was also boosted with increasing Co content.
27659164	8	15	dep	&	1613:1613	arg1	Sons					1615:1618	Sons	1615:1618	Sons	1615:1618	Copyright © 2016 John Wiley & Sons, Ltd.
27659164	4	16	theme	transverse	724:733	arg1	relaxivity					736:745	MRI water (transverse) relaxivity	713:745	MRI water (transverse) relaxivity at 11.7 T	713:755	MRI water (transverse) relaxivity at 11.7 T was also boosted with increasing Co content.
27659164	6	17	theme	dramatic	1267:1274	arg1	effects					1276:1282	quite dramatic effects	1261:1282	quite dramatic effects on cell death of tumor cells	1261:1311	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	3	18	theme	content	612:618	arg1	nanoensembles					581:593	nanoensembles	581:593	nanoensembles of increasing Co content	581:618	With nanoensembles of increasing Co content the saturation magnetization improved, while lattice parameter remained relatively constant.
27659164	7	19	theme	good	1532:1535	arg1	potential					1537:1545	good potential	1532:1545	good potential	1532:1545	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	6	20	theme	heat-induced	1175:1186	arg1	necrosis					1188:1195	heat-induced necrosis	1175:1195	heat-induced necrosis	1175:1195	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	0	21	theme	hyperthermia	48:59	arg1	contrast					69:76	hyperthermia and MRI contrast	48:76	hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles	48:122	Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles.
27659164	5	22	theme	heating	828:834	arg1	Efficiency					802:811	Efficiency	802:811	Efficiency of AMF-induced heating	802:834	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	1	23	theme	ferri/superparamagnetic	139:161	arg1	nanoparticles					163:175	Ferrite-based ferri/superparamagnetic nanoparticles	125:175	Ferrite-based ferri/superparamagnetic nanoparticles	125:175	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	2	24	theme	Co1-x	487:491	arg1	O4					497:498	Fex Co1-x Fe2 O4	483:498	Fex Co1-x Fe2 O4	483:498	Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation.
27659164	0	25	theme	MRI	65:67	arg1	contrast					69:76	hyperthermia and MRI contrast	48:76	hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles	48:122	Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles.
27659164	1	26	theme	resonance	340:348	arg1	contrast					364:371	magnetic resonance imaging (MRI) contrast	331:371	magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity	331:407	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	6	27	with	nanoensembles	1047:1059	arg1	coating					1073:1079	either coating	1066:1079	either coating	1066:1079	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	5	28	theme	Fe2	971:973	arg1	O4					975:976	PEG-coated Fe0.2 Co0.8 Fe2 O4	948:976	PEG-coated Fe0.2 Co0.8 Fe2 O4	948:976	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	0	29	theme	Fex	93:95	arg1	nanoensembles					110:122	hydrophilic Fex Co1-x Fe2 O4 nanoensembles	81:122	hydrophilic Fex Co1-x Fe2 O4 nanoensembles	81:122	Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles.
27659164	4	30	from	11.7 T	750:755	arg1	relaxivity					736:745	MRI water (transverse) relaxivity	713:745	MRI water (transverse) relaxivity at 11.7 T	713:755	MRI water (transverse) relaxivity at 11.7 T was also boosted with increasing Co content.
27659164	2	31	theme	mixed	455:459	arg1	ferrites					468:475	Fe-Co mixed spinel ferrites	449:475	Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation	449:573	Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation.
27659164	5	32	with	comparable	846:855	arg1	PEG					924:926	PEG	924:926	PEG	924:926	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	5	32	with	comparable	846:855	arg1	chitosan					891:898	chitosan	891:898	chitosan	891:898	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	5	32	with	comparable	846:855	arg1	glycol					916:921	polyethylene glycol	903:921	polyethylene glycol (PEG)	903:927	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	5	33	dep	chitosan	891:898	arg1	coating					929:935	coating	929:935	coating	929:935	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	0	34	theme	loss	18:21	arg1	power					23:27	specific loss power	9:27	specific loss power	9:27	Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles.
27659164	1	35	theme	adjacent	280:287	arg1	microenvironment					289:304	the adjacent microenvironment	276:304	the adjacent microenvironment	276:304	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	7	36	theme	Co1-x	1485:1489	arg1	O4					1495:1496	PEG coated Fex Co1-x Fe2 O4	1470:1496	PEG coated Fex Co1-x Fe2 O4 of all the compositions	1470:1520	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	5	37	theme	Fe0.2	959:963	arg1	O4					975:976	PEG-coated Fe0.2 Co0.8 Fe2 O4	948:976	PEG-coated Fe0.2 Co0.8 Fe2 O4	948:976	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	1	38	dep	heated	192:197	arg1	provide					323:329	provide	323:329	provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity	323:407	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	6	39	from	effects	1276:1282	arg1	death					1292:1296	cell death	1287:1296	cell death of tumor cells	1287:1311	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	7	40	theme	coated	1474:1479	arg1	O4					1495:1496	PEG coated Fex Co1-x Fe2 O4	1470:1496	PEG coated Fex Co1-x Fe2 O4 of all the compositions	1470:1520	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	2	41	theme	ferrites	468:475	arg1	nanoensembles					432:444	nanoensembles	432:444	nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation	432:573	Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation.
27659164	3	42	theme	saturation	624:633	arg1	magnetization					635:647	the saturation magnetization	620:647	the saturation magnetization	620:647	With nanoensembles of increasing Co content the saturation magnetization improved, while lattice parameter remained relatively constant.
27659164	3	43	theme	increasing	598:607	arg1	content					612:618	increasing Co content	598:618	increasing Co content	598:618	With nanoensembles of increasing Co content the saturation magnetization improved, while lattice parameter remained relatively constant.
27659164	4	44	theme	Co	790:791	arg1	content					793:799	Co content	790:799	Co content	790:799	MRI water (transverse) relaxivity at 11.7 T was also boosted with increasing Co content.
27659164	6	45	theme	tumor	1096:1100	arg1	cultures					1107:1114	9L tumor cell cultures	1093:1114	9L tumor cell cultures	1093:1114	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	7	46	contain	have	1527:1530	arg2	potential					1537:1545	good potential	1532:1545	good potential	1532:1545	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	7	46	contain	have	1527:1530	arg1	O4					1495:1496	PEG coated Fex Co1-x Fe2 O4	1470:1496	PEG coated Fex Co1-x Fe2 O4 of all the compositions	1470:1520	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	7	46	contain	have	1527:1530	arg1	chitosan					1457:1464	chitosan	1457:1464	chitosan	1457:1464	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	6	47	with	composition	1215:1225	arg1	values					1242:1247	different values	1232:1247	different values of x	1232:1252	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	6	48	theme	x	1252:1252	arg1	values					1242:1247	different values	1232:1247	different values of x	1232:1252	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	1	49	theme	tissue	257:262	arg1	necrosis					264:271	tissue necrosis	257:271	tissue necrosis of the adjacent microenvironment	257:304	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	7	50	theme	mixed	1403:1407	arg1	ferrites					1416:1423	other mixed spinel ferrites	1397:1423	other mixed spinel ferrites	1397:1423	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	7	51	theme	spinel	1409:1414	arg1	ferrites					1416:1423	other mixed spinel ferrites	1397:1423	other mixed spinel ferrites	1397:1423	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	7	52	theme	further	1369:1375	arg1	characterization					1377:1392	further characterization	1369:1392	further characterization of other mixed spinel ferrites	1369:1423	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	6	53	theme	cells	1307:1311	arg1	death					1292:1296	cell death	1287:1296	cell death of tumor cells	1287:1311	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	1	54	theme	enhanced	383:390	arg1	relaxivity					398:407	enhanced water relaxivity	383:407	enhanced water relaxivity	383:407	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	1	55	theme	alternating	214:224	arg1	AMF					242:244	AMF	242:244	AMF	242:244	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	1	55	theme	alternating	214:224	arg1	field					235:239	an external alternating magnetic field	202:239	an external alternating magnetic field (AMF)	202:245	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	7	56	theme	preclinical	1551:1561	arg1	applications					1563:1574	preclinical applications	1551:1574	preclinical applications	1551:1574	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	6	57	theme	Fe2	1208:1210	arg1	composition					1215:1225	Fex Co1-x Fe2 O4 composition	1198:1225	Fex Co1-x Fe2 O4 composition with different values of x	1198:1252	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	6	58	theme	cell	1287:1290	arg1	death					1292:1296	cell death	1287:1296	cell death of tumor cells	1287:1311	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	0	59	theme	O4	107:108	arg1	nanoensembles					110:122	hydrophilic Fex Co1-x Fe2 O4 nanoensembles	81:122	hydrophilic Fex Co1-x Fe2 O4 nanoensembles	81:122	Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles.
27659164	6	60	theme	Fex	1198:1200	arg1	composition					1215:1225	Fex Co1-x Fe2 O4 composition	1198:1225	Fex Co1-x Fe2 O4 composition with different values of x	1198:1252	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	4	61	theme	MRI	713:715	arg1	relaxivity					736:745	MRI water (transverse) relaxivity	713:745	MRI water (transverse) relaxivity at 11.7 T	713:755	MRI water (transverse) relaxivity at 11.7 T was also boosted with increasing Co content.
27659164	3	62	theme	Co	609:610	arg1	content					612:618	increasing Co content	598:618	increasing Co content	598:618	With nanoensembles of increasing Co content the saturation magnetization improved, while lattice parameter remained relatively constant.
27659164	6	63	theme	significant	1126:1136	arg1	differences					1138:1148	no significant differences	1123:1148	no significant differences	1123:1148	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	6	64	dep	necrosis	1188:1195	arg1	i.e.					1170:1173	i.e.	1170:1173	i.e.	1170:1173	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	1	65	theme	Ferrite-based	125:137	arg1	nanoparticles					163:175	Ferrite-based ferri/superparamagnetic nanoparticles	125:175	Ferrite-based ferri/superparamagnetic nanoparticles	125:175	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	7	66	theme	compositions	1509:1520	arg1	O4					1495:1496	PEG coated Fex Co1-x Fe2 O4	1470:1496	PEG coated Fex Co1-x Fe2 O4 of all the compositions	1470:1520	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	7	66	theme	compositions	1509:1520	arg1	chitosan					1457:1464	chitosan	1457:1464	chitosan	1457:1464	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	2	67	theme	Fe2	493:495	arg1	O4					497:498	Fex Co1-x Fe2 O4	483:498	Fex Co1-x Fe2 O4	483:498	Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation.
27659164	1	68	theme	magnetic	331:338	arg1	resonance					340:348	magnetic resonance imaging	331:356	magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity	331:407	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	1	68	theme	magnetic	331:338	arg1	MRI					359:361	MRI	359:361	MRI	359:361	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	5	69	theme	polyethylene	903:914	arg1	PEG					924:926	PEG	924:926	PEG	924:926	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	5	69	theme	polyethylene	903:914	arg1	glycol					916:921	polyethylene glycol	903:921	polyethylene glycol (PEG)	903:927	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	2	70	theme	Fex	483:485	arg1	O4					497:498	Fex Co1-x Fe2 O4	483:498	Fex Co1-x Fe2 O4	483:498	Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation.
27659164	0	71	theme	hydrophilic	81:91	arg1	nanoensembles					110:122	hydrophilic Fex Co1-x Fe2 O4 nanoensembles	81:122	hydrophilic Fex Co1-x Fe2 O4 nanoensembles	81:122	Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles.
27659164	1	72	theme	imaging	350:356	arg1	resonance					340:348	magnetic resonance imaging	331:356	magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity	331:407	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	1	72	theme	imaging	350:356	arg1	MRI					359:361	MRI	359:361	MRI	359:361	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	5	73	theme	Co0.8	965:969	arg1	O4					975:976	PEG-coated Fe0.2 Co0.8 Fe2 O4	948:976	PEG-coated Fe0.2 Co0.8 Fe2 O4	948:976	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	0	74	theme	Co1-x	97:101	arg1	nanoensembles					110:122	hydrophilic Fex Co1-x Fe2 O4 nanoensembles	81:122	hydrophilic Fex Co1-x Fe2 O4 nanoensembles	81:122	Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles.
27659164	0	75	theme	specific	9:16	arg1	power					23:27	specific loss power	9:27	specific loss power	9:27	Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles.
27659164	6	76	theme	nanoensembles	1047:1059	arg1	toxicity					1031:1038	toxicity	1031:1038	toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures	1031:1114	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	2	77	theme	Fe-Co	449:453	arg1	ferrites					468:475	Fe-Co mixed spinel ferrites	449:475	Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation	449:573	Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation.
27659164	5	78	theme	AMF-induced	816:826	arg1	heating					828:834	AMF-induced heating	816:834	AMF-induced heating	816:834	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	5	79	theme	PEG-coated	948:957	arg1	O4					975:976	PEG-coated Fe0.2 Co0.8 Fe2 O4	948:976	PEG-coated Fe0.2 Co0.8 Fe2 O4	948:976	Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2 Co0.8 Fe2 O4 , which was twice as less efficient as others.
27659164	6	80	theme	9L	1093:1094	arg1	cultures					1107:1114	9L tumor cell cultures	1093:1114	9L tumor cell cultures	1093:1114	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	0	81	theme	cancer	32:37	arg1	cells					39:43	cancer cells	32:43	cancer cells	32:43	Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles.
27659164	1	82	theme	microenvironment	289:304	arg1	necrosis					264:271	tissue necrosis	257:271	tissue necrosis of the adjacent microenvironment	257:304	Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity.
27659164	7	83	theme	Fe2	1491:1493	arg1	O4					1495:1496	PEG coated Fex Co1-x Fe2 O4	1470:1496	PEG coated Fex Co1-x Fe2 O4 of all the compositions	1470:1520	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	7	84	theme	Fex	1481:1483	arg1	O4					1495:1496	PEG coated Fex Co1-x Fe2 O4	1470:1496	PEG coated Fex Co1-x Fe2 O4 of all the compositions	1470:1520	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	2	85	theme	spinel	461:466	arg1	ferrites					468:475	Fe-Co mixed spinel ferrites	449:475	Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation	449:573	Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation.
27659164	3	86	theme	lattice	665:671	arg1	parameter					673:681	lattice parameter	665:681	lattice parameter	665:681	With nanoensembles of increasing Co content the saturation magnetization improved, while lattice parameter remained relatively constant.
27659164	2	87	dep	O4	497:498	arg1	i.e.					478:481	i.e.	478:481	i.e.	478:481	Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation.
27659164	7	88	theme	PEG	1470:1472	arg1	O4					1495:1496	PEG coated Fex Co1-x Fe2 O4	1470:1496	PEG coated Fex Co1-x Fe2 O4 of all the compositions	1470:1520	This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated Fex Co1-x Fe2 O4 of all the compositions will have good potential for preclinical applications in vivo.
27659164	6	89	theme	cell	1102:1105	arg1	cultures					1107:1114	9L tumor cell cultures	1093:1114	9L tumor cell cultures	1093:1114	While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) Fex Co1-x Fe2 O4 composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings.
27659164	0	90	theme	Fe2	103:105	arg1	nanoensembles					110:122	hydrophilic Fex Co1-x Fe2 O4 nanoensembles	81:122	hydrophilic Fex Co1-x Fe2 O4 nanoensembles	81:122	Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic Fex Co1-x Fe2 O4 nanoensembles.
28135049	0	0	theme	Collagen	101:108	arg1	Networks					110:117	3D Collagen Networks	98:117	3D Collagen Networks	98:117	Instructing Human Macrophage Polarization by Stiffness and Glycosaminoglycan Functionalization in 3D Collagen Networks.
28135049	7	1	theme	dimensionality	1205:1218	arg1	influence					1192:1200	a strong influence	1183:1200	a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization	1183:1295	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	1	2	theme	extracellular	176:188	arg1	matrix					190:195	the extracellular matrix	172:195	the extracellular matrix	172:195	Dynamic alterations of composition and mechanics of the extracellular matrix are suggested to modulate cellular behavior including plasticity of macrophages (MPhs) during wound healing.
28135049	6	3	dep	matrices	1007:1014	arg1	stress					1016:1021	stress	1016:1021	stress	1016:1021	Furthermore, control experiments on 2D matrices stress the relevance of using stiffness-controlled 3D matrices, as MPhs show a reciprocal polarization behavior depending on GAG presence.
28135049	0	4	theme	3D	98:99	arg1	Networks					110:117	3D Collagen Networks	98:117	3D Collagen Networks	98:117	Instructing Human Macrophage Polarization by Stiffness and Glycosaminoglycan Functionalization in 3D Collagen Networks.
28135049	1	5	theme	matrix	190:195	arg1	mechanics					159:167	mechanics	159:167	mechanics	159:167	Dynamic alterations of composition and mechanics of the extracellular matrix are suggested to modulate cellular behavior including plasticity of macrophages (MPhs) during wound healing.
28135049	1	5	theme	matrix	190:195	arg1	composition					143:153	composition	143:153	composition	143:153	Dynamic alterations of composition and mechanics of the extracellular matrix are suggested to modulate cellular behavior including plasticity of macrophages (MPhs) during wound healing.
28135049	3	6	dep	proinflammatory	675:689	arg1	phenotypes					708:717	phenotypes	708:717	phenotypes	708:717	Human MPhs are found to sensitively respond to these microenvironmental cues in terms of polarization toward proinflammatory or wound healing phenotypes over 6 days in vitro.
28135049	2	7	theme	different	531:539	arg1	stages					541:546	different stages	531:546	different stages of wound healing	531:563	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	7	8	theme	3D	1355:1356	arg1	context					1366:1372	the 3D in vivo context	1351:1372	the 3D in vivo context	1351:1372	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	2	9	dep	biopolymers	383:393	arg1	glycosaminoglycans					408:425	glycosaminoglycans	408:425	glycosaminoglycans (GAGs)	408:432	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	2	9	dep	biopolymers	383:393	arg1	collagen					396:403	collagen I	396:405	collagen I	396:405	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	3	10	theme	Human	566:570	arg1	MPhs					572:575	Human MPhs	566:575	Human MPhs	566:575	Human MPhs are found to sensitively respond to these microenvironmental cues in terms of polarization toward proinflammatory or wound healing phenotypes over 6 days in vitro.
28135049	1	11	theme	wound	291:295	arg1	healing					297:303	wound healing	291:303	wound healing	291:303	Dynamic alterations of composition and mechanics of the extracellular matrix are suggested to modulate cellular behavior including plasticity of macrophages (MPhs) during wound healing.
28135049	5	12	theme	GAGs	928:931	arg1	Presence					891:898	Presence	891:898	Presence of sulfated and nonsulfated GAGs	891:931	Presence of sulfated and nonsulfated GAGs inhibits this polarization effect.
28135049	2	13	used	used	439:442	arg2	matrices					345:352	engineered 3D fibrillar matrices	321:352	engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs))	321:433	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	7	14	theme	in	1423:1424	arg1	studies					1432:1438	in vitro studies	1423:1438	in vitro studies	1423:1438	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	7	15	theme	GAG	1236:1238	arg1	presence					1240:1247	GAG presence	1236:1247	GAG presence	1236:1247	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	4	16	theme	healing	762:768	arg1	phenotype					770:778	a wound healing phenotype	754:778	a wound healing phenotype	754:778	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	6	17	theme	polarization	1106:1117	arg1	behavior					1119:1126	a reciprocal polarization behavior	1093:1126	a reciprocal polarization behavior	1093:1126	Furthermore, control experiments on 2D matrices stress the relevance of using stiffness-controlled 3D matrices, as MPhs show a reciprocal polarization behavior depending on GAG presence.
28135049	4	18	theme	relevant	848:855	arg1	cytokines					857:865	relevant cytokines	848:865	relevant cytokines (IL10, IL12, and TNFα)	848:888	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	4	18	theme	relevant	848:855	arg1	TNFα					884:887	TNFα	884:887	TNFα	884:887	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	4	18	theme	relevant	848:855	arg1	IL10					868:871	IL10	868:871	IL10	868:871	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	7	19	from	composition	1408:1418	arg1	studies					1432:1438	in vitro studies	1423:1438	in vitro studies	1423:1438	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	4	20	theme	stiffer	783:789	arg1	matrices					791:798	stiffer matrices	783:798	stiffer matrices	783:798	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	7	21	dep	in	1358:1359	arg1	vivo					1361:1364	vivo	1361:1364	vivo	1361:1364	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	2	22	theme	nonsulfated	511:521	arg1	GAGs					523:526	sulfated and nonsulfated GAGs	498:526	GAGs	523:526	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	5	23	theme	nonsulfated	916:926	arg1	GAGs					928:931	sulfated and nonsulfated GAGs	903:931	sulfated and nonsulfated GAGs	903:931	Presence of sulfated and nonsulfated GAGs inhibits this polarization effect.
28135049	0	24	theme	Human	12:16	arg1	Polarization					29:40	Human Macrophage Polarization	12:40	Human Macrophage Polarization by Stiffness and Glycosaminoglycan Functionalization in 3D Collagen Networks	12:117	Instructing Human Macrophage Polarization by Stiffness and Glycosaminoglycan Functionalization in 3D Collagen Networks.
28135049	3	25	theme	in	731:732	arg1	days					726:729	6 days	724:729	6 days in vitro	724:738	Human MPhs are found to sensitively respond to these microenvironmental cues in terms of polarization toward proinflammatory or wound healing phenotypes over 6 days in vitro.
28135049	2	26	theme	fibrillar	335:343	arg1	matrices					345:352	engineered 3D fibrillar matrices	321:352	engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs))	321:433	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	7	27	theme	biomaterial	1256:1266	arg1	scaffold					1268:1275	the biomaterial scaffold	1252:1275	the biomaterial scaffold	1252:1275	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	4	28	dep	cytokines	857:865	arg1	cytokines					857:865	relevant cytokines	848:865	relevant cytokines (IL10, IL12, and TNFα)	848:888	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	4	28	dep	cytokines	857:865	arg1	IL12					874:877	IL12	874:877	IL12	874:877	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	4	28	dep	cytokines	857:865	arg1	TNFα					884:887	TNFα	884:887	TNFα	884:887	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	4	28	dep	cytokines	857:865	arg1	IL10					868:871	IL10	868:871	IL10	868:871	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	4	29	theme	cytokines	857:865	arg1	expression					834:843	protein and gene expression	817:843	expression	834:843	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	2	30	theme	3D	332:333	arg1	matrices					345:352	engineered 3D fibrillar matrices	321:352	engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs))	321:433	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	6	31	theme	3D	1067:1068	arg1	matrices					1070:1077	stiffness-controlled 3D matrices	1046:1077	stiffness-controlled 3D matrices	1046:1077	Furthermore, control experiments on 2D matrices stress the relevance of using stiffness-controlled 3D matrices, as MPhs show a reciprocal polarization behavior depending on GAG presence.
28135049	2	32	theme	healing	557:563	arg1	stages					541:546	different stages	531:546	different stages of wound healing	531:563	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	6	33	theme	GAG	1141:1143	arg1	presence					1145:1152	GAG presence	1141:1152	GAG presence	1141:1152	Furthermore, control experiments on 2D matrices stress the relevance of using stiffness-controlled 3D matrices, as MPhs show a reciprocal polarization behavior depending on GAG presence.
28135049	2	34	theme	engineered	321:330	arg1	matrices					345:352	engineered 3D fibrillar matrices	321:352	engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs))	321:433	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	1	35	theme	cellular	223:230	arg1	behavior					232:239	cellular behavior	223:239	cellular behavior	223:239	Dynamic alterations of composition and mechanics of the extracellular matrix are suggested to modulate cellular behavior including plasticity of macrophages (MPhs) during wound healing.
28135049	0	36	theme	Macrophage	18:27	arg1	Polarization					29:40	Human Macrophage Polarization	12:40	Human Macrophage Polarization by Stiffness and Glycosaminoglycan Functionalization in 3D Collagen Networks	12:117	Instructing Human Macrophage Polarization by Stiffness and Glycosaminoglycan Functionalization in 3D Collagen Networks.
28135049	2	37	theme	wound	551:555	arg1	healing					557:563	wound healing	551:563	wound healing	551:563	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	2	38	theme	occurring	373:381	arg1	biopolymers					383:393	naturally occurring biopolymers	363:393	naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs))	363:433	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	7	39	theme	presence	1240:1247	arg1	influence					1192:1200	a strong influence	1183:1200	a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization	1183:1295	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	3	40	theme	polarization	655:666	arg1	terms					646:650	terms	646:650	terms of polarization toward proinflammatory or wound healing phenotypes over 6 days in vitro	646:738	Human MPhs are found to sensitively respond to these microenvironmental cues in terms of polarization toward proinflammatory or wound healing phenotypes over 6 days in vitro.
28135049	7	41	theme	MPh	1280:1282	arg1	polarization					1284:1295	MPh polarization	1280:1295	MPh polarization	1280:1295	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	7	42	from	influence	1192:1200	arg1	polarization					1284:1295	MPh polarization	1280:1295	MPh polarization	1280:1295	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	4	43	theme	protein	817:823	arg1	expression					834:843	protein and gene expression	817:843	expression	834:843	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	4	44	theme	wound	756:760	arg1	phenotype					770:778	a wound healing phenotype	754:778	a wound healing phenotype	754:778	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	0	45	theme	Glycosaminoglycan	59:75	arg1	Functionalization					77:93	Glycosaminoglycan Functionalization	59:93	Glycosaminoglycan Functionalization	59:93	Instructing Human Macrophage Polarization by Stiffness and Glycosaminoglycan Functionalization in 3D Collagen Networks.
28135049	3	46	dep	in	731:732	arg1	vitro					734:738	vitro	734:738	vitro	734:738	Human MPhs are found to sensitively respond to these microenvironmental cues in terms of polarization toward proinflammatory or wound healing phenotypes over 6 days in vitro.
28135049	7	47	theme	in	1358:1359	arg1	context					1366:1372	the 3D in vivo context	1351:1372	the 3D in vivo context	1351:1372	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	7	48	theme	scaffold	1268:1275	arg1	presence					1240:1247	GAG presence	1236:1247	GAG presence	1236:1247	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	7	48	theme	scaffold	1268:1275	arg1	stiffness					1221:1229	stiffness	1221:1229	stiffness	1221:1229	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	7	48	theme	scaffold	1268:1275	arg1	dimensionality					1205:1218	dimensionality	1205:1218	dimensionality	1205:1218	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	6	49	from	experiments	989:999	arg1	matrices					1007:1014	2D matrices	1004:1014	2D matrices	1004:1014	Furthermore, control experiments on 2D matrices stress the relevance of using stiffness-controlled 3D matrices, as MPhs show a reciprocal polarization behavior depending on GAG presence.
28135049	4	50	theme	gene	829:832	arg1	expression					834:843	protein and gene expression	817:843	expression	834:843	MPhs exhibit a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, and TNFα).
28135049	7	51	theme	strong	1185:1190	arg1	influence					1192:1200	a strong influence	1183:1200	a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization	1183:1295	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	1	52	theme	composition	143:153	arg1	alterations					128:138	Dynamic alterations	120:138	Dynamic alterations of composition and mechanics of the extracellular matrix	120:195	Dynamic alterations of composition and mechanics of the extracellular matrix are suggested to modulate cellular behavior including plasticity of macrophages (MPhs) during wound healing.
28135049	3	53	theme	wound	694:698	arg1	healing					700:706	wound healing	694:706	wound healing	694:706	Human MPhs are found to sensitively respond to these microenvironmental cues in terms of polarization toward proinflammatory or wound healing phenotypes over 6 days in vitro.
28135049	3	54	theme	microenvironmental	619:636	arg1	cues					638:641	these microenvironmental cues	613:641	these microenvironmental cues	613:641	Human MPhs are found to sensitively respond to these microenvironmental cues in terms of polarization toward proinflammatory or wound healing phenotypes over 6 days in vitro.
28135049	7	55	theme	GAG	1404:1406	arg1	composition					1408:1418	GAG composition	1404:1418	GAG composition	1404:1418	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	5	56	theme	sulfated	903:910	arg1	GAGs					928:931	sulfated and nonsulfated GAGs	903:931	sulfated and nonsulfated GAGs	903:931	Presence of sulfated and nonsulfated GAGs inhibits this polarization effect.
28135049	1	57	theme	Dynamic	120:126	arg1	alterations					128:138	Dynamic alterations	120:138	Dynamic alterations of composition and mechanics of the extracellular matrix	120:195	Dynamic alterations of composition and mechanics of the extracellular matrix are suggested to modulate cellular behavior including plasticity of macrophages (MPhs) during wound healing.
28135049	6	58	theme	control	981:987	arg1	experiments					989:999	control experiments	981:999	control experiments on 2D matrices stress the relevance of using stiffness-controlled 3D matrices, as MPhs	981:1086	Furthermore, control experiments on 2D matrices stress the relevance of using stiffness-controlled 3D matrices, as MPhs show a reciprocal polarization behavior depending on GAG presence.
28135049	5	59	theme	polarization	947:958	arg1	effect					960:965	this polarization effect	942:965	this polarization effect	942:965	Presence of sulfated and nonsulfated GAGs inhibits this polarization effect.
28135049	1	60	theme	mechanics	159:167	arg1	alterations					128:138	Dynamic alterations	120:138	Dynamic alterations of composition and mechanics of the extracellular matrix	120:195	Dynamic alterations of composition and mechanics of the extracellular matrix are suggested to modulate cellular behavior including plasticity of macrophages (MPhs) during wound healing.
28135049	7	61	from	stiffness	1390:1398	arg1	studies					1432:1438	in vitro studies	1423:1438	in vitro studies	1423:1438	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	1	62	theme	macrophages	265:275	arg1	plasticity					251:260	plasticity	251:260	plasticity of macrophages (MPhs) during wound healing	251:303	Dynamic alterations of composition and mechanics of the extracellular matrix are suggested to modulate cellular behavior including plasticity of macrophages (MPhs) during wound healing.
28135049	7	63	theme	stiffness	1221:1229	arg1	influence					1192:1200	a strong influence	1183:1200	a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization	1183:1295	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	6	64	theme	stiffness-controlled	1046:1065	arg1	matrices					1070:1077	stiffness-controlled 3D matrices	1046:1077	stiffness-controlled 3D matrices	1046:1077	Furthermore, control experiments on 2D matrices stress the relevance of using stiffness-controlled 3D matrices, as MPhs show a reciprocal polarization behavior depending on GAG presence.
28135049	6	65	theme	reciprocal	1095:1104	arg1	behavior					1119:1126	a reciprocal polarization behavior	1093:1126	a reciprocal polarization behavior	1093:1126	Furthermore, control experiments on 2D matrices stress the relevance of using stiffness-controlled 3D matrices, as MPhs show a reciprocal polarization behavior depending on GAG presence.
28135049	7	66	dep	in	1423:1424	arg1	vitro					1426:1430	vitro	1426:1430	vitro	1426:1430	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	2	67	theme	sulfated	498:505	arg1	GAGs					523:526	sulfated and nonsulfated GAGs	498:526	GAGs	523:526	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	0	68	from	Polarization	29:40	arg1	Networks					110:117	3D Collagen Networks	98:117	3D Collagen Networks	98:117	Instructing Human Macrophage Polarization by Stiffness and Glycosaminoglycan Functionalization in 3D Collagen Networks.
28135049	2	69	theme	matrix	453:458	arg1	stiffening					460:469	matrix stiffening	453:469	matrix stiffening as well as modification by sulfated and nonsulfated GAGs	453:526	In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) are used to mimic matrix stiffening as well as modification by sulfated and nonsulfated GAGs at different stages of wound healing.
28135049	7	70	theme	variable	1381:1388	arg1	stiffness					1390:1398	a variable stiffness	1379:1398	a variable stiffness	1379:1398	Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
28135049	6	71	theme	2D	1004:1005	arg1	matrices					1007:1014	2D matrices	1004:1014	2D matrices	1004:1014	Furthermore, control experiments on 2D matrices stress the relevance of using stiffness-controlled 3D matrices, as MPhs show a reciprocal polarization behavior depending on GAG presence.
25152492	7	0	theme	nm	1463:1464	arg1	density					1359:1365	proper wet density	1348:1365	proper wet density of 1.64-2.78 g/ml	1348:1383	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	0	theme	nm	1463:1464	arg1	porosity					1414:1421	porosity	1414:1421	porosity of 98-90%	1414:1431	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	0	theme	nm	1463:1464	arg1	content					1392:1398	water content	1386:1398	water content of 62.74-34%	1386:1411	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	0	theme	nm	1463:1464	arg1	size					1442:1445	pore size	1437:1445	pore size of about 38-130 nm	1437:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	0	theme	nm	1463:1464	arg1	μm					1344:1345	126.81-151.47 μm	1330:1345	126.81-151.47 μm	1330:1345	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	1	theme	normal	1253:1258	arg1	distribution					1265:1276	the logarithmic normal size distribution	1237:1276	the logarithmic normal size distribution	1237:1276	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	9	2	theme	obtained	1817:1824	arg1	results					1826:1832	obtained results	1817:1832	obtained results	1817:1832	According to obtained results the expansion factors under the same fluid velocity decreased by increasing the matrix density.
25152492	8	3	theme	other	1788:1792	arg1	matrices					1794:1801	other matrices	1788:1801	other matrices	1788:1801	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	3	4	theme	Agar-Agar	539:547	arg1	polymer					549:555	cost-effective adsorbent with favorable qualities Agar-Agar polymer	489:555	cost-effective adsorbent with favorable qualities Agar-Agar polymer	489:555	In order to fabricate cost-effective adsorbent with favorable qualities Agar-Agar polymer was used.
25152492	13	5	theme	adsorption	2611:2620	arg1	qm					2632:2633	qm	2632:2633	qm	2632:2633	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	13	5	theme	adsorption	2611:2620	arg1	capacity					2622:2629	the maximum adsorption capacity	2599:2629	the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent)	2599:2680	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	13	5	theme	adsorption	2611:2620	arg1	higher					2686:2691	higher	2686:2691	higher	2686:2691	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	11	6	theme	fluid	2303:2307	arg1	operation					2292:2300	high operation	2287:2300	high operation	2287:2300	It was observed that the application of matrix with high density was proper for high operation, fluid velocity, since the addition of densifier improves the rigidity of the matrix.
25152492	11	6	theme	fluid	2303:2307	arg1	velocity					2309:2316	fluid velocity	2303:2316	fluid velocity	2303:2316	It was observed that the application of matrix with high density was proper for high operation, fluid velocity, since the addition of densifier improves the rigidity of the matrix.
25152492	11	7	theme	matrix	2247:2252	arg1	proper					2276:2281	proper	2276:2281	proper	2276:2281	It was observed that the application of matrix with high density was proper for high operation, fluid velocity, since the addition of densifier improves the rigidity of the matrix.
25152492	11	7	theme	matrix	2247:2252	arg1	application					2232:2242	the application	2228:2242	the application of matrix with high density	2228:2270	It was observed that the application of matrix with high density was proper for high operation, fluid velocity, since the addition of densifier improves the rigidity of the matrix.
25152492	4	8	theme	pseudo-affinity	629:643	arg1	RB4					674:676	RB4	674:676	RB4	674:676	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	4	8	theme	pseudo-affinity	629:643	arg1	dye-ligand					645:654	pseudo-affinity dye-ligand	629:654	pseudo-affinity dye-ligand	629:654	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	13	9	theme	Langmuir	2572:2579	arg1	isotherm					2581:2588	a typical Langmuir isotherm	2562:2588	a typical Langmuir isotherm	2562:2588	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	7	10	theme	size	1442:1445	arg1	μm					1303:1304	65-300 μm	1296:1304	65-300 μm	1296:1304	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	10	theme	size	1442:1445	arg1	diameter					1318:1325	average diameter	1310:1325	average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm	1310:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	11	11	theme	high	2259:2262	arg1	density					2264:2270	high density	2259:2270	high density	2259:2270	It was observed that the application of matrix with high density was proper for high operation, fluid velocity, since the addition of densifier improves the rigidity of the matrix.
25152492	10	12	theme	bed	2054:2056	arg1	stability					2032:2040	the stability	2028:2040	the stability of expanded bed	2028:2056	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	3	13	with	adsorbent	504:512	arg1	qualities					529:537	favorable qualities	519:537	favorable qualities	519:537	In order to fabricate cost-effective adsorbent with favorable qualities Agar-Agar polymer was used.
25152492	1	14	theme	nanoporous	189:198	arg1	matrix					210:215	dense nanoporous composite matrix	183:215	dense nanoporous composite matrix for expanded bed application	183:244	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	11	15	with	application	2232:2242	arg1	density					2264:2270	high density	2259:2270	high density	2259:2270	It was observed that the application of matrix with high density was proper for high operation, fluid velocity, since the addition of densifier improves the rigidity of the matrix.
25152492	4	16	theme	customized	583:592	arg1	particle					604:611	the customized composite particle	579:611	the customized composite particle	579:611	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	18	17	theme	high	3386:3389	arg1	stability					3391:3399	high stability	3386:3399	high stability	3386:3399	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	14	18	theme	kinetic	2783:2789	arg1	processes					2802:2810	kinetic adsorption processes	2783:2810	kinetic adsorption processes	2783:2810	Additionally kinetic adsorption processes were characterized by the pseudo-first-order and pseudo-second-order kinetics equations.
25152492	1	19	theme	matrix	210:215	arg1	densifier					307:315	a densifier	305:315	a densifier	305:315	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	1	19	theme	matrix	210:215	arg1	skeleton					359:366	a skeleton	357:366	a skeleton	357:366	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	1	19	theme	matrix	210:215	arg1	kind					175:178	a novel kind	167:178	a novel kind of dense nanoporous composite matrix for expanded bed application	167:244	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	6	20	theme	electronic	1159:1168	arg1	microscope					1170:1179	scanning electronic microscope	1150:1179	scanning electronic microscope (SEM)	1150:1185	Spherical appearance and porous structure of composite particles were observed by the optical microscope (OM) and scanning electronic microscope (SEM).
25152492	6	20	theme	electronic	1159:1168	arg1	SEM					1182:1184	SEM	1182:1184	SEM	1182:1184	Spherical appearance and porous structure of composite particles were observed by the optical microscope (OM) and scanning electronic microscope (SEM).
25152492	9	21	theme	fluid	1871:1875	arg1	velocity					1877:1884	the same fluid velocity	1862:1884	the same fluid velocity	1862:1884	According to obtained results the expansion factors under the same fluid velocity decreased by increasing the matrix density.
25152492	8	22	theme	retention	1698:1706	arg1	method					1726:1731	the retention time distribution method	1694:1731	the retention time distribution method (RTD)	1694:1737	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	8	22	theme	retention	1698:1706	arg1	RTD					1734:1736	RTD	1734:1736	RTD	1734:1736	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	1	23	theme	expanded	221:228	arg1	application					234:244	expanded bed application	221:244	expanded bed application	221:244	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	12	24	theme	momentous	2394:2402	arg1	pH					2413:2414	pH	2413:2414	pH	2413:2414	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	12	24	theme	momentous	2394:2402	arg1	strength					2423:2430	ionic strength	2417:2430	ionic strength	2417:2430	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	12	24	theme	momentous	2394:2402	arg1	factors					2404:2410	Three momentous factors	2388:2410	Three momentous factors	2388:2410	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	12	24	theme	momentous	2394:2402	arg1	concentration					2444:2456	initial concentration	2436:2456	initial concentration	2436:2456	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	1	25	theme	emulsification	404:417	arg1	method					381:386	the method	377:386	the method of water-in-oil emulsification	377:417	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	8	26	theme	distribution	1713:1724	arg1	method					1726:1731	the retention time distribution method	1694:1731	the retention time distribution method (RTD)	1694:1737	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	8	26	theme	distribution	1713:1724	arg1	RTD					1734:1736	RTD	1734:1736	RTD	1734:1736	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	7	27	theme	density	1359:1365	arg1	μm					1303:1304	65-300 μm	1296:1304	65-300 μm	1296:1304	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	27	theme	density	1359:1365	arg1	diameter					1318:1325	average diameter	1310:1325	average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm	1310:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	9	28	theme	matrix	1914:1919	arg1	density					1921:1927	the matrix density	1910:1927	the matrix density	1910:1927	According to obtained results the expansion factors under the same fluid velocity decreased by increasing the matrix density.
25152492	4	29	theme	bioprodut	745:753	arg1	adsorption					755:764	bioprodut adsorption	745:764	bioprodut adsorption from aqueous solution	745:786	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	5	30	theme	obtained	941:948	arg1	results					950:956	the obtained results	937:956	the obtained results	937:956	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	6	31	theme	Spherical	1036:1044	arg1	appearance					1046:1055	Spherical appearance	1036:1055	Spherical appearance	1036:1055	Spherical appearance and porous structure of composite particles were observed by the optical microscope (OM) and scanning electronic microscope (SEM).
25152492	7	32	theme	proper	1348:1353	arg1	density					1359:1365	proper wet density	1348:1365	proper wet density of 1.64-2.78 g/ml	1348:1383	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	13	33	theme	equilibrium	2524:2534	arg1	isotherms					2536:2544	the adsorption equilibrium isotherms	2509:2544	the adsorption equilibrium isotherms	2509:2544	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	10	34	theme	axial	1944:1948	arg1	parameter					2003:2011	the most appropriate parameter	1982:2011	the most appropriate parameter for evaluating the stability of expanded bed	1982:2056	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	34	theme	axial	1944:1948	arg1	Dax					1974:1976	Dax	1974:1976	Dax	1974:1976	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	34	theme	axial	1944:1948	arg1	coefficient					1961:1971	the axial dispersion coefficient	1940:1971	the axial dispersion coefficient (Dax)	1940:1977	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	5	35	theme	Bovine	789:794	arg1	protein					840:846	a model protein	832:846	a model protein	832:846	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	5	35	theme	Bovine	789:794	arg1	BSA					811:813	BSA	811:813	BSA	811:813	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	5	35	theme	Bovine	789:794	arg1	Albumin					802:808	Bovine Serum Albumin	789:808	Bovine Serum Albumin (BSA)	789:814	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	13	36	theme	best	2484:2487	arg1	results					2489:2495	The best results	2480:2495	The best results	2480:2495	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	5	37	theme	expanded	904:911	arg1	chromatography					917:930	expanded bed chromatography	904:930	expanded bed chromatography	904:930	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	18	38	theme	bioproduct	3324:3333	arg1	adsorption					3335:3344	efficient bioproduct adsorption	3314:3344	efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it	3314:3406	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	10	39	theme	flow	2110:2113	arg1	velocity					2115:2122	different flow velocity	2100:2122	different flow velocity	2100:2122	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	4	40	mod	modified	617:624	arg3	RB4					674:676	RB4	674:676	RB4	674:676	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	4	40	mod	modified	617:624	arg3	dye-ligand					645:654	pseudo-affinity dye-ligand	629:654	pseudo-affinity dye-ligand	629:654	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	4	40	mod	modified	617:624	arg1	particle					604:611	the customized composite particle	579:611	the customized composite particle	579:611	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	16	41	theme	breakthrough	2982:2993	arg1	curves					2995:3000	the breakthrough curves	2978:3000	the breakthrough curves	2978:3000	Also the breakthrough curves were investigated.
25152492	8	42	theme	better	1471:1476	arg1	comprehension					1478:1490	better comprehension	1471:1490	better comprehension of the impact of solid phase properties on the performance of the expanded bed	1471:1569	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	17	43	dep	2.78	3237:3240	arg1	to					3234:3235	to	3234:3235	to	3234:3235	It was found that dynamic binding capacity (DBC) decreased with increasing the flow rate and the values of DBC decreased from 21.08 to 11.15 mg/ml adsorbent when the density of composite beads increased from 1.64 to 2.78 g/ml.
25152492	0	44	theme	expanded	118:125	arg1	chromatography					131:144	expanded bed chromatography	118:144	expanded bed chromatography	118:144	Cost-effective nanoporous Agar-Agar polymer/Nickel powder composite particle for effective bio-products adsorption by expanded bed chromatography.
25152492	17	45	dep	11.15	3156:3160	arg1	to					3153:3154	to	3153:3154	to	3153:3154	It was found that dynamic binding capacity (DBC) decreased with increasing the flow rate and the values of DBC decreased from 21.08 to 11.15 mg/ml adsorbent when the density of composite beads increased from 1.64 to 2.78 g/ml.
25152492	13	46	theme	adsorbent	2671:2679	arg1	RB4-Agar-Ni					2646:2656	RB4-Agar-Ni	2646:2656	RB4-Agar-Ni (64.01 mg/ml adsorbent)	2646:2680	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	13	46	theme	adsorbent	2671:2679	arg1	mg/ml					2665:2669	64.01 mg/ml adsorbent	2659:2679	64.01 mg/ml adsorbent	2659:2679	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	18	47	theme	hydrodynamic	3356:3367	arg1	characteristics					3369:3383	good hydrodynamic characteristics	3351:3383	good hydrodynamic characteristics	3351:3383	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	10	48	theme	expansion	2129:2137	arg1	degree					2139:2144	bed expansion degree	2125:2144	bed expansion degree	2125:2144	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	49	from	parameter	2003:2011	arg1	density					2185:2191	the density	2181:2191	the density of adsorbent	2181:2204	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	49	from	parameter	2003:2011	arg1	velocity					2115:2122	different flow velocity	2100:2122	different flow velocity	2100:2122	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	49	from	parameter	2003:2011	arg1	viscosity					2147:2155	viscosity	2147:2155	viscosity of the liquid phase	2147:2175	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	49	from	parameter	2003:2011	arg1	degree					2139:2144	bed expansion degree	2125:2144	bed expansion degree	2125:2144	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	49	from	parameter	2003:2011	arg1	conditions					2080:2089	various operating conditions	2062:2089	various operating conditions	2062:2089	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	4	50	from	solution	779:786	arg1	adsorption					755:764	bioprodut adsorption	745:764	bioprodut adsorption from aqueous solution	745:786	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	18	51	theme	prepared	3278:3285	arg1	promising					3300:3308	promising	3300:3308	promising	3300:3308	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	18	51	theme	prepared	3278:3285	arg1	composite					3287:3295	the prepared composite	3274:3295	the prepared composite	3274:3295	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	8	52	theme	impact	1499:1504	arg1	comprehension					1478:1490	better comprehension	1471:1490	better comprehension of the impact of solid phase properties on the performance of the expanded bed	1471:1569	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	10	53	theme	liquid	2164:2169	arg1	phase					2171:2175	the liquid phase	2160:2175	the liquid phase	2160:2175	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	0	54	theme	Cost-effective	0:13	arg1	Agar-Agar					26:34	Cost-effective nanoporous Agar-Agar	0:34	Cost-effective nanoporous Agar-Agar	0:34	Cost-effective nanoporous Agar-Agar polymer/Nickel powder composite particle for effective bio-products adsorption by expanded bed chromatography.
25152492	6	55	theme	composite	1081:1089	arg1	particles					1091:1099	composite particles	1081:1099	composite particles	1081:1099	Spherical appearance and porous structure of composite particles were observed by the optical microscope (OM) and scanning electronic microscope (SEM).
25152492	2	56	theme	inexpensive	446:456	arg1	polymer					458:464	a porous and inexpensive polymer	433:464	a porous and inexpensive polymer	433:464	Agar-Agar is a porous and inexpensive polymer.
25152492	2	56	theme	inexpensive	446:456	arg1	Agar-Agar					420:428	Agar-Agar	420:428	Agar-Agar	420:428	Agar-Agar is a porous and inexpensive polymer.
25152492	8	57	theme	solid	1509:1513	arg1	properties					1521:1530	solid phase properties	1509:1530	solid phase properties	1509:1530	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	8	58	with	matrix	1629:1634	arg1	series					1643:1648	a series	1641:1648	a series of densities	1641:1661	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	12	59	theme	BSA	2461:2463	arg1	pH					2413:2414	pH	2413:2414	pH	2413:2414	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	12	59	theme	BSA	2461:2463	arg1	strength					2423:2430	ionic strength	2417:2430	ionic strength	2417:2430	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	12	59	theme	BSA	2461:2463	arg1	concentration					2444:2456	initial concentration	2436:2456	initial concentration	2436:2456	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	12	59	theme	BSA	2461:2463	arg1	factors					2404:2410	Three momentous factors	2388:2410	Three momentous factors	2388:2410	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	17	60	theme	composite	3198:3206	arg1	beads					3208:3212	composite beads	3198:3212	composite beads	3198:3212	It was found that dynamic binding capacity (DBC) decreased with increasing the flow rate and the values of DBC decreased from 21.08 to 11.15 mg/ml adsorbent when the density of composite beads increased from 1.64 to 2.78 g/ml.
25152492	2	61	theme	porous	435:440	arg1	polymer					458:464	a porous and inexpensive polymer	433:464	a porous and inexpensive polymer	433:464	Agar-Agar is a porous and inexpensive polymer.
25152492	2	61	theme	porous	435:440	arg1	Agar-Agar					420:428	Agar-Agar	420:428	Agar-Agar	420:428	Agar-Agar is a porous and inexpensive polymer.
25152492	5	62	theme	model	834:838	arg1	protein					840:846	a model protein	832:846	a model protein	832:846	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	5	62	theme	model	834:838	arg1	Albumin					802:808	Bovine Serum Albumin	789:808	Bovine Serum Albumin (BSA)	789:814	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	7	63	theme	diameter	1318:1325	arg1	range					1287:1291	the range	1283:1291	the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm	1283:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	4	64	theme	composite	594:602	arg1	particle					604:611	the customized composite particle	579:611	the customized composite particle	579:611	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	6	65	theme	porous	1061:1066	arg1	structure					1068:1076	porous structure	1061:1076	porous structure	1061:1076	Spherical appearance and porous structure of composite particles were observed by the optical microscope (OM) and scanning electronic microscope (SEM).
25152492	1	66	theme	dense	183:187	arg1	matrix					210:215	dense nanoporous composite matrix	183:215	dense nanoporous composite matrix for expanded bed application	183:244	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	0	67	theme	powder	51:56	arg1	particle					68:75	powder composite particle	51:75	powder composite particle for effective bio-products adsorption	51:113	Cost-effective nanoporous Agar-Agar polymer/Nickel powder composite particle for effective bio-products adsorption by expanded bed chromatography.
25152492	7	68	theme	logarithmic	1241:1251	arg1	distribution					1265:1276	the logarithmic normal size distribution	1237:1276	the logarithmic normal size distribution	1237:1276	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	3	69	theme	cost-effective	489:502	arg1	polymer					549:555	cost-effective adsorbent with favorable qualities Agar-Agar polymer	489:555	cost-effective adsorbent with favorable qualities Agar-Agar polymer	489:555	In order to fabricate cost-effective adsorbent with favorable qualities Agar-Agar polymer was used.
25152492	1	70	theme	novel	169:173	arg1	densifier					307:315	a densifier	305:315	a densifier	305:315	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	1	70	theme	novel	169:173	arg1	skeleton					359:366	a skeleton	357:366	a skeleton	357:366	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	1	70	theme	novel	169:173	arg1	kind					175:178	a novel kind	167:178	a novel kind of dense nanoporous composite matrix for expanded bed application	167:244	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	12	71	theme	initial	2436:2442	arg1	concentration					2444:2456	initial concentration	2436:2456	initial concentration	2436:2456	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	12	71	theme	initial	2436:2442	arg1	factors					2404:2410	Three momentous factors	2388:2410	Three momentous factors	2388:2410	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	8	72	theme	expanded	1558:1565	arg1	bed					1567:1569	the expanded bed	1554:1569	the expanded bed	1554:1569	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	8	73	theme	composite	1619:1627	arg1	matrix					1629:1634	a composite matrix	1617:1634	a composite matrix with a series of densities	1617:1661	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	13	74	theme	adsorbent	2758:2766	arg1	mg.ml					2752:2756	about 54 mg.ml adsorbent	2743:2766	about 54 mg.ml adsorbent	2743:2766	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	13	74	theme	adsorbent	2758:2766	arg1	adsorbent					2732:2740	adsorbent	2732:2740	adsorbent	2732:2740	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	6	75	theme	optical	1122:1128	arg1	OM					1142:1143	OM	1142:1143	OM	1142:1143	Spherical appearance and porous structure of composite particles were observed by the optical microscope (OM) and scanning electronic microscope (SEM).
25152492	6	75	theme	optical	1122:1128	arg1	microscope					1130:1139	the optical microscope	1118:1139	the optical microscope (OM)	1118:1144	Spherical appearance and porous structure of composite particles were observed by the optical microscope (OM) and scanning electronic microscope (SEM).
25152492	13	76	theme	maximum	2603:2609	arg1	qm					2632:2633	qm	2632:2633	qm	2632:2633	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	13	76	theme	maximum	2603:2609	arg1	capacity					2622:2629	the maximum adsorption capacity	2599:2629	the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent)	2599:2680	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	13	76	theme	maximum	2603:2609	arg1	higher					2686:2691	higher	2686:2691	higher	2686:2691	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	14	77	theme	adsorption	2791:2800	arg1	processes					2802:2810	kinetic adsorption processes	2783:2810	kinetic adsorption processes	2783:2810	Additionally kinetic adsorption processes were characterized by the pseudo-first-order and pseudo-second-order kinetics equations.
25152492	7	78	theme	size	1260:1263	arg1	distribution					1265:1276	the logarithmic normal size distribution	1237:1276	the logarithmic normal size distribution	1237:1276	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	1	79	theme	Nickel	288:293	arg1	powder					295:300	Nickel powder	288:300	Nickel powder	288:300	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	7	80	theme	pore	1437:1440	arg1	size					1442:1445	pore size	1437:1445	pore size of about 38-130 nm	1437:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	13	81	from	capacity	2622:2629	arg1	RB4-Agar-Ni					2646:2656	RB4-Agar-Ni	2646:2656	RB4-Agar-Ni (64.01 mg/ml adsorbent)	2646:2680	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	13	81	from	capacity	2622:2629	arg1	mg/ml					2665:2669	64.01 mg/ml adsorbent	2659:2679	64.01 mg/ml adsorbent	2659:2679	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	9	82	theme	expansion	1838:1846	arg1	factors					1848:1854	the expansion factors	1834:1854	the expansion factors under the same fluid velocity	1834:1884	According to obtained results the expansion factors under the same fluid velocity decreased by increasing the matrix density.
25152492	8	83	theme	hydrodynamic	1590:1601	arg1	properties					1603:1612	hydrodynamic properties	1590:1612	hydrodynamic properties	1590:1612	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	18	84	with	adsorption	3335:3344	arg1	it					3405:3406	it	3405:3406	it	3405:3406	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	18	84	with	adsorption	3335:3344	arg1	characteristics					3369:3383	good hydrodynamic characteristics	3351:3383	good hydrodynamic characteristics	3351:3383	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	18	84	with	adsorption	3335:3344	arg1	stability					3391:3399	high stability	3386:3399	high stability	3386:3399	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	8	85	theme	densities	1653:1661	arg1	series					1643:1648	a series	1641:1648	a series of densities	1641:1661	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	5	86	theme	adsorption	867:876	arg1	behavior					878:885	the adsorption behavior	863:885	the adsorption behavior in batchwise and expanded bed chromatography	863:930	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	7	87	theme	%	1431:1431	arg1	density					1359:1365	proper wet density	1348:1365	proper wet density of 1.64-2.78 g/ml	1348:1383	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	87	theme	%	1431:1431	arg1	porosity					1414:1421	porosity	1414:1421	porosity of 98-90%	1414:1431	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	87	theme	%	1431:1431	arg1	content					1392:1398	water content	1386:1398	water content of 62.74-34%	1386:1411	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	87	theme	%	1431:1431	arg1	size					1442:1445	pore size	1437:1445	pore size of about 38-130 nm	1437:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	87	theme	%	1431:1431	arg1	μm					1344:1345	126.81-151.47 μm	1330:1345	126.81-151.47 μm	1330:1345	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	88	theme	porosity	1414:1421	arg1	μm					1303:1304	65-300 μm	1296:1304	65-300 μm	1296:1304	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	88	theme	porosity	1414:1421	arg1	diameter					1318:1325	average diameter	1310:1325	average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm	1310:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	10	89	theme	expanded	2045:2052	arg1	bed					2054:2056	expanded bed	2045:2056	expanded bed	2045:2056	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	8	90	from	performance	1539:1549	arg1	comprehension					1478:1490	better comprehension	1471:1490	better comprehension of the impact of solid phase properties on the performance of the expanded bed	1471:1569	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	1	91	theme	composite	200:208	arg1	matrix					210:215	dense nanoporous composite matrix	183:215	dense nanoporous composite matrix for expanded bed application	183:244	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	10	92	theme	adsorbent	2196:2204	arg1	density					2185:2191	the density	2181:2191	the density of adsorbent	2181:2204	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	92	theme	adsorbent	2196:2204	arg1	velocity					2115:2122	different flow velocity	2100:2122	different flow velocity	2100:2122	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	92	theme	adsorbent	2196:2204	arg1	viscosity					2147:2155	viscosity	2147:2155	viscosity of the liquid phase	2147:2175	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	92	theme	adsorbent	2196:2204	arg1	degree					2139:2144	bed expansion degree	2125:2144	bed expansion degree	2125:2144	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	7	93	theme	%	1411:1411	arg1	density					1359:1365	proper wet density	1348:1365	proper wet density of 1.64-2.78 g/ml	1348:1383	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	93	theme	%	1411:1411	arg1	porosity					1414:1421	porosity	1414:1421	porosity of 98-90%	1414:1431	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	93	theme	%	1411:1411	arg1	content					1392:1398	water content	1386:1398	water content of 62.74-34%	1386:1411	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	93	theme	%	1411:1411	arg1	size					1442:1445	pore size	1437:1445	pore size of about 38-130 nm	1437:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	93	theme	%	1411:1411	arg1	μm					1344:1345	126.81-151.47 μm	1330:1345	126.81-151.47 μm	1330:1345	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	9	94	theme	same	1866:1869	arg1	velocity					1877:1884	the same fluid velocity	1862:1884	the same fluid velocity	1862:1884	According to obtained results the expansion factors under the same fluid velocity decreased by increasing the matrix density.
25152492	17	95	theme	DBC	3128:3130	arg1	values					3118:3123	the values	3114:3123	the values of DBC	3114:3130	It was found that dynamic binding capacity (DBC) decreased with increasing the flow rate and the values of DBC decreased from 21.08 to 11.15 mg/ml adsorbent when the density of composite beads increased from 1.64 to 2.78 g/ml.
25152492	8	96	from	impact	1499:1504	arg1	performance					1539:1549	the performance	1535:1549	the performance of the expanded bed	1535:1569	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	6	97	theme	scanning	1150:1157	arg1	microscope					1170:1179	scanning electronic microscope	1150:1179	scanning electronic microscope (SEM)	1150:1185	Spherical appearance and porous structure of composite particles were observed by the optical microscope (OM) and scanning electronic microscope (SEM).
25152492	6	97	theme	scanning	1150:1157	arg1	SEM					1182:1184	SEM	1182:1184	SEM	1182:1184	Spherical appearance and porous structure of composite particles were observed by the optical microscope (OM) and scanning electronic microscope (SEM).
25152492	10	98	theme	various	2062:2068	arg1	density					2185:2191	the density	2181:2191	the density of adsorbent	2181:2204	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	98	theme	various	2062:2068	arg1	velocity					2115:2122	different flow velocity	2100:2122	different flow velocity	2100:2122	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	98	theme	various	2062:2068	arg1	viscosity					2147:2155	viscosity	2147:2155	viscosity of the liquid phase	2147:2175	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	98	theme	various	2062:2068	arg1	degree					2139:2144	bed expansion degree	2125:2144	bed expansion degree	2125:2144	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	98	theme	various	2062:2068	arg1	conditions					2080:2089	various operating conditions	2062:2089	various operating conditions	2062:2089	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	18	99	theme	expanded	3424:3431	arg1	usage					3437:3441	expanded bed usage	3424:3441	expanded bed usage	3424:3441	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	1	100	theme	water-in-oil	391:402	arg1	emulsification					404:417	water-in-oil emulsification	391:417	water-in-oil emulsification	391:417	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	13	101	theme	typical	2564:2570	arg1	isotherm					2581:2588	a typical Langmuir isotherm	2562:2588	a typical Langmuir isotherm	2562:2588	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	1	102	theme	bed	230:232	arg1	application					234:244	expanded bed application	221:244	expanded bed application	221:244	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	7	103	theme	water	1386:1390	arg1	content					1392:1398	water content	1386:1398	water content of 62.74-34%	1386:1411	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	10	104	theme	different	2100:2108	arg1	velocity					2115:2122	different flow velocity	2100:2122	different flow velocity	2100:2122	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	8	105	theme	time	1708:1711	arg1	method					1726:1731	the retention time distribution method	1694:1731	the retention time distribution method (RTD)	1694:1737	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	8	105	theme	time	1708:1711	arg1	RTD					1734:1736	RTD	1734:1736	RTD	1734:1736	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	17	106	theme	dynamic	3039:3045	arg1	DBC					3065:3067	DBC	3065:3067	DBC	3065:3067	It was found that dynamic binding capacity (DBC) decreased with increasing the flow rate and the values of DBC decreased from 21.08 to 11.15 mg/ml adsorbent when the density of composite beads increased from 1.64 to 2.78 g/ml.
25152492	17	106	theme	dynamic	3039:3045	arg1	capacity					3055:3062	dynamic binding capacity	3039:3062	dynamic binding capacity (DBC)	3039:3068	It was found that dynamic binding capacity (DBC) decreased with increasing the flow rate and the values of DBC decreased from 21.08 to 11.15 mg/ml adsorbent when the density of composite beads increased from 1.64 to 2.78 g/ml.
25152492	7	107	theme	g/ml	1380:1383	arg1	density					1359:1365	proper wet density	1348:1365	proper wet density of 1.64-2.78 g/ml	1348:1383	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	107	theme	g/ml	1380:1383	arg1	porosity					1414:1421	porosity	1414:1421	porosity of 98-90%	1414:1431	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	107	theme	g/ml	1380:1383	arg1	content					1392:1398	water content	1386:1398	water content of 62.74-34%	1386:1411	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	107	theme	g/ml	1380:1383	arg1	size					1442:1445	pore size	1437:1445	pore size of about 38-130 nm	1437:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	107	theme	g/ml	1380:1383	arg1	μm					1344:1345	126.81-151.47 μm	1330:1345	126.81-151.47 μm	1330:1345	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	1	108	theme	present	154:160	arg1	work					162:165	the present work	150:165	the present work	150:165	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	7	109	theme	content	1392:1398	arg1	μm					1303:1304	65-300 μm	1296:1304	65-300 μm	1296:1304	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	109	theme	content	1392:1398	arg1	diameter					1318:1325	average diameter	1310:1325	average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm	1310:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	10	110	theme	bed	2125:2127	arg1	degree					2139:2144	bed expansion degree	2125:2144	bed expansion degree	2125:2144	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	111	theme	operating	2070:2078	arg1	density					2185:2191	the density	2181:2191	the density of adsorbent	2181:2204	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	111	theme	operating	2070:2078	arg1	velocity					2115:2122	different flow velocity	2100:2122	different flow velocity	2100:2122	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	111	theme	operating	2070:2078	arg1	viscosity					2147:2155	viscosity	2147:2155	viscosity of the liquid phase	2147:2175	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	111	theme	operating	2070:2078	arg1	degree					2139:2144	bed expansion degree	2125:2144	bed expansion degree	2125:2144	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	111	theme	operating	2070:2078	arg1	conditions					2080:2089	various operating conditions	2062:2089	various operating conditions	2062:2089	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	112	theme	dispersion	1950:1959	arg1	parameter					2003:2011	the most appropriate parameter	1982:2011	the most appropriate parameter for evaluating the stability of expanded bed	1982:2056	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	112	theme	dispersion	1950:1959	arg1	Dax					1974:1976	Dax	1974:1976	Dax	1974:1976	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	112	theme	dispersion	1950:1959	arg1	coefficient					1961:1971	the axial dispersion coefficient	1940:1971	the axial dispersion coefficient (Dax)	1940:1977	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	0	113	theme	bio-products	91:102	arg1	adsorption					104:113	effective bio-products adsorption	81:113	effective bio-products adsorption	81:113	Cost-effective nanoporous Agar-Agar polymer/Nickel powder composite particle for effective bio-products adsorption by expanded bed chromatography.
25152492	12	114	theme	ionic	2417:2421	arg1	strength					2423:2430	ionic strength	2417:2430	ionic strength	2417:2430	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	12	114	theme	ionic	2417:2421	arg1	factors					2404:2410	Three momentous factors	2388:2410	Three momentous factors	2388:2410	Three momentous factors, pH, ionic strength and initial concentration of BSA were analyzed.
25152492	7	115	theme	wet	1355:1357	arg1	density					1359:1365	proper wet density	1348:1365	proper wet density of 1.64-2.78 g/ml	1348:1383	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	17	116	theme	flow	3100:3103	arg1	rate					3105:3108	the flow rate	3096:3108	the flow rate	3096:3108	It was found that dynamic binding capacity (DBC) decreased with increasing the flow rate and the values of DBC decreased from 21.08 to 11.15 mg/ml adsorbent when the density of composite beads increased from 1.64 to 2.78 g/ml.
25152492	5	117	theme	Serum	796:800	arg1	protein					840:846	a model protein	832:846	a model protein	832:846	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	5	117	theme	Serum	796:800	arg1	BSA					811:813	BSA	811:813	BSA	811:813	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	5	117	theme	Serum	796:800	arg1	Albumin					802:808	Bovine Serum Albumin	789:808	Bovine Serum Albumin (BSA)	789:814	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	5	118	from	behavior	878:885	arg1	chromatography					917:930	expanded bed chromatography	904:930	expanded bed chromatography	904:930	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	5	118	from	behavior	878:885	arg1	batchwise					890:898	batchwise	890:898	batchwise	890:898	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	5	119	theme	bed	913:915	arg1	chromatography					917:930	expanded bed chromatography	904:930	expanded bed chromatography	904:930	Bovine Serum Albumin (BSA) was selected as a model protein to investigate the adsorption behavior in batchwise and expanded bed chromatography, and the obtained results were evaluated with that of Streamline™ (Amersham-Pharmacia Biotech, Sweden).
25152492	18	120	theme	efficient	3314:3322	arg1	adsorption					3335:3344	efficient bioproduct adsorption	3314:3344	efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it	3314:3406	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	4	121	theme	adsorbent	717:725	arg1	pseudo-affinity					701:715	a pseudo-affinity adsorbent	699:725	a pseudo-affinity adsorbent (RB4-Agar-Ni)	699:739	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	4	121	theme	adsorbent	717:725	arg1	RB4-Agar-Ni					728:738	RB4-Agar-Ni	728:738	RB4-Agar-Ni	728:738	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	0	122	theme	bed	127:129	arg1	chromatography					131:144	expanded bed chromatography	118:144	expanded bed chromatography	118:144	Cost-effective nanoporous Agar-Agar polymer/Nickel powder composite particle for effective bio-products adsorption by expanded bed chromatography.
25152492	8	123	theme	expanded	1745:1752	arg1	bed					1754:1756	an expanded bed	1742:1756	an expanded bed	1742:1756	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	17	124	theme	adsorbent	3168:3176	arg1	mg/ml					3162:3166	21.08 to 11.15 mg/ml adsorbent	3147:3176	21.08 to 11.15 mg/ml adsorbent	3147:3176	It was found that dynamic binding capacity (DBC) decreased with increasing the flow rate and the values of DBC decreased from 21.08 to 11.15 mg/ml adsorbent when the density of composite beads increased from 1.64 to 2.78 g/ml.
25152492	7	125	theme	μm	1344:1345	arg1	μm					1303:1304	65-300 μm	1296:1304	65-300 μm	1296:1304	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	7	125	theme	μm	1344:1345	arg1	diameter					1318:1325	average diameter	1310:1325	average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm	1310:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	18	126	theme	good	3351:3354	arg1	characteristics					3369:3383	good hydrodynamic characteristics	3351:3383	good hydrodynamic characteristics	3351:3383	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	11	127	theme	matrix	2380:2385	arg1	rigidity					2364:2371	the rigidity	2360:2371	the rigidity of the matrix	2360:2385	It was observed that the application of matrix with high density was proper for high operation, fluid velocity, since the addition of densifier improves the rigidity of the matrix.
25152492	10	128	theme	phase	2171:2175	arg1	density					2185:2191	the density	2181:2191	the density of adsorbent	2181:2204	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	128	theme	phase	2171:2175	arg1	velocity					2115:2122	different flow velocity	2100:2122	different flow velocity	2100:2122	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	128	theme	phase	2171:2175	arg1	viscosity					2147:2155	viscosity	2147:2155	viscosity of the liquid phase	2147:2175	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	128	theme	phase	2171:2175	arg1	degree					2139:2144	bed expansion degree	2125:2144	bed expansion degree	2125:2144	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	0	129	theme	nanoporous	15:24	arg1	Agar-Agar					26:34	Cost-effective nanoporous Agar-Agar	0:34	Cost-effective nanoporous Agar-Agar	0:34	Cost-effective nanoporous Agar-Agar polymer/Nickel powder composite particle for effective bio-products adsorption by expanded bed chromatography.
25152492	14	130	dep	pseudo-first-order	2838:2855	arg1	equations					2890:2898	equations	2890:2898	equations	2890:2898	Additionally kinetic adsorption processes were characterized by the pseudo-first-order and pseudo-second-order kinetics equations.
25152492	6	131	theme	particles	1091:1099	arg1	structure					1068:1076	porous structure	1061:1076	porous structure	1061:1076	Spherical appearance and porous structure of composite particles were observed by the optical microscope (OM) and scanning electronic microscope (SEM).
25152492	6	131	theme	particles	1091:1099	arg1	appearance					1046:1055	Spherical appearance	1036:1055	Spherical appearance	1036:1055	Spherical appearance and porous structure of composite particles were observed by the optical microscope (OM) and scanning electronic microscope (SEM).
25152492	8	132	theme	phase	1515:1519	arg1	properties					1521:1530	solid phase properties	1509:1530	solid phase properties	1509:1530	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	11	133	theme	high	2287:2290	arg1	operation					2292:2300	high operation	2287:2300	high operation	2287:2300	It was observed that the application of matrix with high density was proper for high operation, fluid velocity, since the addition of densifier improves the rigidity of the matrix.
25152492	11	133	theme	high	2287:2290	arg1	velocity					2309:2316	fluid velocity	2303:2316	fluid velocity	2303:2316	It was observed that the application of matrix with high density was proper for high operation, fluid velocity, since the addition of densifier improves the rigidity of the matrix.
25152492	7	134	theme	average	1310:1316	arg1	diameter					1318:1325	average diameter	1310:1325	average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm	1310:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	4	135	theme	aqueous	771:777	arg1	solution					779:786	aqueous solution	771:786	aqueous solution	771:786	Thereafter, the customized composite particle was modified by pseudo-affinity dye-ligand, Reactive Blue 4 (RB4), aimed at preparing a pseudo-affinity adsorbent (RB4-Agar-Ni) for bioprodut adsorption from aqueous solution.
25152492	17	136	theme	beads	3208:3212	arg1	density					3187:3193	the density	3183:3193	the density of composite beads	3183:3212	It was found that dynamic binding capacity (DBC) decreased with increasing the flow rate and the values of DBC decreased from 21.08 to 11.15 mg/ml adsorbent when the density of composite beads increased from 1.64 to 2.78 g/ml.
25152492	0	137	theme	composite	58:66	arg1	particle					68:75	powder composite particle	51:75	powder composite particle for effective bio-products adsorption	51:113	Cost-effective nanoporous Agar-Agar polymer/Nickel powder composite particle for effective bio-products adsorption by expanded bed chromatography.
25152492	7	138	theme	μm	1303:1304	arg1	range					1287:1291	the range	1283:1291	the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm	1283:1464	The results suggested that the matrices followed the logarithmic normal size distribution with the range of 65-300 μm and average diameter of 126.81-151.47 μm, proper wet density of 1.64-2.78 g/ml, water content of 62.74-34%, porosity of 98-90% and pore size of about 38-130 nm.
25152492	15	139	theme	experimental	2905:2916	arg1	data					2918:2921	The experimental data	2901:2921	The experimental data	2901:2921	The experimental data followed the pseudo-first-order kinetic equation.
25152492	17	140	theme	binding	3047:3053	arg1	DBC					3065:3067	DBC	3065:3067	DBC	3065:3067	It was found that dynamic binding capacity (DBC) decreased with increasing the flow rate and the values of DBC decreased from 21.08 to 11.15 mg/ml adsorbent when the density of composite beads increased from 1.64 to 2.78 g/ml.
25152492	17	140	theme	binding	3047:3053	arg1	capacity					3055:3062	dynamic binding capacity	3039:3062	dynamic binding capacity (DBC)	3039:3068	It was found that dynamic binding capacity (DBC) decreased with increasing the flow rate and the values of DBC decreased from 21.08 to 11.15 mg/ml adsorbent when the density of composite beads increased from 1.64 to 2.78 g/ml.
25152492	15	141	theme	kinetic	2955:2961	arg1	equation					2963:2970	the pseudo-first-order kinetic equation	2932:2970	the pseudo-first-order kinetic equation	2932:2970	The experimental data followed the pseudo-first-order kinetic equation.
25152492	14	142	theme	pseudo-second-order	2861:2879	arg1	kinetics					2881:2888	pseudo-second-order kinetics	2861:2888	pseudo-second-order kinetics	2861:2888	Additionally kinetic adsorption processes were characterized by the pseudo-first-order and pseudo-second-order kinetics equations.
25152492	0	143	theme	effective	81:89	arg1	adsorption					104:113	effective bio-products adsorption	81:113	effective bio-products adsorption	81:113	Cost-effective nanoporous Agar-Agar polymer/Nickel powder composite particle for effective bio-products adsorption by expanded bed chromatography.
25152492	13	144	theme	adsorption	2513:2522	arg1	isotherms					2536:2544	the adsorption equilibrium isotherms	2509:2544	the adsorption equilibrium isotherms	2509:2544	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	8	145	theme	properties	1521:1530	arg1	impact					1499:1504	the impact	1495:1504	the impact of solid phase properties on the performance of the expanded bed	1495:1569	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	3	146	theme	favorable	519:527	arg1	qualities					529:537	favorable qualities	519:537	favorable qualities	519:537	In order to fabricate cost-effective adsorbent with favorable qualities Agar-Agar polymer was used.
25152492	1	147	theme	Agar-Agar	338:346	arg1	layer					348:352	Agar-Agar layer	338:352	Agar-Agar layer	338:352	In the present work a novel kind of dense nanoporous composite matrix for expanded bed application has been successfully first prepared with Nickel powder as a densifier and was covered with Agar-Agar layer as a skeleton, through the method of water-in-oil emulsification.
25152492	13	148	theme	BSA	2639:2641	arg1	qm					2632:2633	qm	2632:2633	qm	2632:2633	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	13	148	theme	BSA	2639:2641	arg1	capacity					2622:2629	the maximum adsorption capacity	2599:2629	the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent)	2599:2680	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	13	148	theme	BSA	2639:2641	arg1	higher					2686:2691	higher	2686:2691	higher	2686:2691	The best results showed that the adsorption equilibrium isotherms seems to follow a typical Langmuir isotherm and also the maximum adsorption capacity (qm) of BSA on RB4-Agar-Ni (64.01 mg/ml adsorbent) was higher than that on RB4-Streamline commercial adsorbent (about 54 mg.ml adsorbent).
25152492	10	149	theme	appropriate	1991:2001	arg1	parameter					2003:2011	the most appropriate parameter	1982:2011	the most appropriate parameter for evaluating the stability of expanded bed	1982:2056	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	10	149	theme	appropriate	1991:2001	arg1	coefficient					1961:1971	the axial dispersion coefficient	1940:1971	the axial dispersion coefficient (Dax)	1940:1977	Moreover, the axial dispersion coefficient (Dax) is the most appropriate parameter for evaluating the stability of expanded bed, on various operating conditions, such as different flow velocity, bed expansion degree, viscosity of the liquid phase and the density of adsorbent.
25152492	8	150	theme	bed	1567:1569	arg1	performance					1539:1549	the performance	1535:1549	the performance of the expanded bed	1535:1569	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	3	151	theme	adsorbent	504:512	arg1	polymer					549:555	cost-effective adsorbent with favorable qualities Agar-Agar polymer	489:555	cost-effective adsorbent with favorable qualities Agar-Agar polymer	489:555	In order to fabricate cost-effective adsorbent with favorable qualities Agar-Agar polymer was used.
25152492	8	152	theme	matrix	1629:1634	arg1	expansion					1576:1584	the expansion	1572:1584	the expansion	1572:1584	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	8	152	theme	matrix	1629:1634	arg1	properties					1603:1612	hydrodynamic properties	1590:1612	hydrodynamic properties	1590:1612	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
25152492	11	153	theme	densifier	2341:2349	arg1	addition					2329:2336	the addition	2325:2336	the addition of densifier	2325:2349	It was observed that the application of matrix with high density was proper for high operation, fluid velocity, since the addition of densifier improves the rigidity of the matrix.
25152492	15	154	theme	pseudo-first-order	2936:2953	arg1	equation					2963:2970	the pseudo-first-order kinetic equation	2932:2970	the pseudo-first-order kinetic equation	2932:2970	The experimental data followed the pseudo-first-order kinetic equation.
25152492	18	155	theme	bed	3433:3435	arg1	usage					3437:3441	expanded bed usage	3424:3441	expanded bed usage	3424:3441	All results indicate that the prepared composite is promising for efficient bioproduct adsorption with good hydrodynamic characteristics, high stability and it is suitable for expanded bed usage as a cost-effective adsorbent.
25152492	8	156	from	comprehension	1478:1490	arg1	performance					1539:1549	the performance	1535:1549	the performance of the expanded bed	1535:1569	For better comprehension of the impact of solid phase properties on the performance of the expanded bed, the expansion and hydrodynamic properties of a composite matrix with a series of densities was evaluated and estimated by the retention time distribution method (RTD) in an expanded bed and was compared with that of other matrices.
28166248	4	0	theme	underlying	938:947	arg1	tissue					960:965	the underlying connective tissue	934:965	the underlying connective tissue	934:965	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	5	1	theme	migration	990:998	arg1	speed					1000:1004	migration speed	990:1004	migration speed	990:1004	Our quantification of migration speed confirms a biphasic role of Matrigel.
28166248	4	2	theme	basement	912:919	arg1	membrane					921:928	a disrupted basement membrane	900:928	a disrupted basement membrane	900:928	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	4	3	theme	lung	733:736	arg1	cancer					738:743	lung cancer cell migration, which turns from mesenchymal in collagen only matrices,	733:815	cancer	738:743	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	2	4	theme	collagen-Matrigel	322:338	arg1	composition					340:350	mixed collagen-Matrigel composition	316:350	mixed collagen-Matrigel composition	316:350	In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments.
28166248	6	5	theme	low	1047:1049	arg1	concentration					1051:1063	low concentration	1047:1063	low concentration	1047:1063	At low concentration, Matrigel facilitates migration, most probably by providing a supportive and growth factor retaining environment.
28166248	3	6	theme	adenocarcinoma	632:645	arg1	cells					654:658	H1299 lung adenocarcinoma cancer cells	621:658	H1299 lung adenocarcinoma cancer cells	621:658	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	10	7	theme	precise	1847:1853	arg1	devices					1838:1844	microfluidic devices	1825:1844	microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs	1825:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	7	theme	precise	1847:1853	arg1	control					1855:1861	precise control	1847:1861	precise control of the environment	1847:1880	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	3	8	dep	different	663:671	arg1	experimental					673:684	experimental	673:684	experimental	673:684	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	8	9	theme	integrin	1305:1312	arg1	blockade					1314:1321	antibody-based integrin blockade	1290:1321	antibody-based integrin blockade	1290:1321	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	3	10	theme	cells	654:658	arg1	migration					608:616	the migration	604:616	the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions	604:695	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	1	11	theme	tissues	239:245	arg1	behavior					217:224	the behavior	213:224	the behavior of cells and tissues	213:245	Microfluidic devices are becoming mainstream tools to recapitulate in vitro the behavior of cells and tissues.
28166248	10	12	theme	environment	1870:1880	arg1	devices					1838:1844	microfluidic devices	1825:1844	microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs	1825:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	12	theme	environment	1870:1880	arg1	control					1855:1861	precise control	1847:1861	precise control of the environment	1847:1880	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	12	theme	environment	1870:1880	arg1	parallelization					1913:1927	parallelization	1913:1927	parallelization for high throughput studies	1913:1955	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	12	theme	environment	1870:1880	arg1	properties					1901:1910	excellent optical properties	1883:1910	excellent optical properties	1883:1910	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	12	theme	environment	1870:1880	arg1	use					1971:1973	efficient use	1961:1973	efficient use of therapeutic drugs	1961:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	8	13	from	change	1334:1339	arg1	lobopodial					1384:1393	lobopodial	1384:1393	lobopodial	1384:1393	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	8	13	from	change	1334:1339	arg1	mesenchymal					1369:1379	mesenchymal	1369:1379	mesenchymal	1369:1379	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	8	13	from	change	1334:1339	arg1	phenotype					1354:1362	migration phenotype	1344:1362	migration phenotype from mesenchymal or lobopodial to amoeboid	1344:1405	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	5	14	theme	speed	1000:1004	arg1	quantification					972:985	Our quantification	968:985	Our quantification of migration speed	968:1004	Our quantification of migration speed confirms a biphasic role of Matrigel.
28166248	3	15	from	migration	608:616	arg1	conditions					686:695	different experimental conditions	663:695	different experimental conditions	663:695	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	4	16	theme	cell	745:748	arg1	migration					750:758	cell migration	745:758	cell migration	745:758	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	0	17	theme	collagen-Matrigel	69:85	arg1	scaffolds					87:95	mixed collagen-Matrigel scaffolds	63:95	mixed collagen-Matrigel scaffolds using microfluidics and image analysis	63:134	Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis.
28166248	3	18	theme	different	663:671	arg1	conditions					686:695	different experimental conditions	663:695	different experimental conditions	663:695	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	9	19	theme	tools	1605:1609	arg1	platform					1574:1581	a robust microfluidic platform	1552:1581	a robust microfluidic platform	1552:1581	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	9	19	theme	tools	1605:1609	arg1	set					1589:1591	a set	1587:1591	a set of software tools	1587:1609	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	9	19	theme	tools	1605:1609	arg1	tools					1605:1609	software tools	1596:1609	software tools	1596:1609	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	0	20	from	Characterization	0:15	arg1	scaffolds					87:95	mixed collagen-Matrigel scaffolds	63:95	mixed collagen-Matrigel scaffolds using microfluidics and image analysis	63:134	Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis.
28166248	8	21	theme	change	1438:1443	arg1	effect					1423:1428	the effect	1419:1428	the effect of this change in migration dynamics, in regards to the structure of the matrix	1419:1508	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	9	22	theme	cancer	1642:1647	arg1	migration					1654:1662	lung cancer cell migration	1637:1662	lung cancer cell migration	1637:1662	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	10	23	used	used	1750:1753	arg2	platform					1732:1739	This platform	1727:1739	This platform	1727:1739	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	8	24	theme	migration	1448:1456	arg1	dynamics					1458:1465	migration dynamics	1448:1465	migration dynamics	1448:1465	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	2	25	theme	invasion	419:426	arg1	microenvironments					428:444	different cancer invasion microenvironments	402:444	different cancer invasion microenvironments	402:444	In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments.
28166248	4	26	from	matrices	807:814	arg1	mesenchymal					778:788	mesenchymal	778:788	mesenchymal	778:788	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	4	27	dep	cancer	738:743	arg1	migration					750:758	cell migration	745:758	cell migration	745:758	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	6	28	theme	retaining	1156:1164	arg1	environment					1166:1176	a supportive and growth factor retaining environment	1125:1176	a supportive and growth factor retaining environment	1125:1176	At low concentration, Matrigel facilitates migration, most probably by providing a supportive and growth factor retaining environment.
28166248	4	29	theme	collagen	793:800	arg1	matrices					807:814	collagen only matrices	793:814	collagen only matrices	793:814	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	2	30	theme	different	402:410	arg1	microenvironments					428:444	different cancer invasion microenvironments	402:444	different cancer invasion microenvironments	402:444	In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments.
28166248	10	31	theme	future	1758:1763	arg1	studies					1765:1771	future studies	1758:1771	future studies	1758:1771	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	0	32	theme	three-dimensional	20:36	arg1	migration					50:58	three-dimensional cancer cell migration	20:58	three-dimensional cancer cell migration	20:58	Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis.
28166248	3	33	theme	image	578:582	arg1	analysis					584:591	quantitative image analysis	565:591	quantitative image analysis	565:591	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	5	34	theme	Matrigel	1034:1041	arg1	role					1026:1029	a biphasic role	1015:1029	a biphasic role of Matrigel	1015:1041	Our quantification of migration speed confirms a biphasic role of Matrigel.
28166248	6	35	theme	supportive	1127:1136	arg1	environment					1166:1176	a supportive and growth factor retaining environment	1125:1176	a supportive and growth factor retaining environment	1125:1176	At low concentration, Matrigel facilitates migration, most probably by providing a supportive and growth factor retaining environment.
28166248	3	36	theme	microfluidic	476:487	arg1	device					489:494	the microfluidic device	472:494	the microfluidic device	472:494	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	0	37	theme	cell	45:48	arg1	migration					50:58	three-dimensional cancer cell migration	20:58	three-dimensional cancer cell migration	20:58	Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis.
28166248	10	38	theme	high	1933:1936	arg1	studies					1949:1955	high throughput studies	1933:1955	high throughput studies	1933:1955	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	1	39	theme	Microfluidic	137:148	arg1	devices					150:156	Microfluidic devices	137:156	Microfluidic devices	137:156	Microfluidic devices are becoming mainstream tools to recapitulate in vitro the behavior of cells and tissues.
28166248	9	40	theme	different	1670:1678	arg1	microenvironments					1680:1696	different microenvironments	1670:1696	different microenvironments	1670:1696	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	8	41	theme	matrix	1503:1508	arg1	structure					1486:1494	the structure	1482:1494	the structure of the matrix	1482:1508	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	10	42	theme	efficient	1961:1969	arg1	devices					1838:1844	microfluidic devices	1825:1844	microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs	1825:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	42	theme	efficient	1961:1969	arg1	use					1971:1973	efficient use	1961:1973	efficient use of therapeutic drugs	1961:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	2	43	theme	cells	390:394	arg1	migration					365:373	the migration	361:373	the migration of lung cancer cells	361:394	In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments.
28166248	2	44	theme	lung	378:381	arg1	cells					390:394	lung cancer cells	378:394	lung cancer cells	378:394	In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments.
28166248	1	45	theme	cells	229:233	arg1	behavior					217:224	the behavior	213:224	the behavior of cells and tissues	213:245	Microfluidic devices are becoming mainstream tools to recapitulate in vitro the behavior of cells and tissues.
28166248	10	46	theme	drugs	1990:1994	arg1	devices					1838:1844	microfluidic devices	1825:1844	microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs	1825:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	46	theme	drugs	1990:1994	arg1	control					1855:1861	precise control	1847:1861	precise control of the environment	1847:1880	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	46	theme	drugs	1990:1994	arg1	parallelization					1913:1927	parallelization	1913:1927	parallelization for high throughput studies	1913:1955	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	46	theme	drugs	1990:1994	arg1	properties					1901:1910	excellent optical properties	1883:1910	excellent optical properties	1883:1910	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	46	theme	drugs	1990:1994	arg1	use					1971:1973	efficient use	1961:1973	efficient use of therapeutic drugs	1961:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	8	47	from	dynamics	1458:1465	arg1	effect					1423:1428	the effect	1419:1428	the effect of this change in migration dynamics, in regards to the structure of the matrix	1419:1508	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	4	48	theme	connective	949:958	arg1	tissue					960:965	the underlying connective tissue	934:965	the underlying connective tissue	934:965	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	7	49	theme	high	1182:1185	arg1	concentration					1187:1199	high concentration	1182:1199	high concentration	1182:1199	At high concentration, Matrigel slows down migration, possibly due excessive attachment.
28166248	10	50	theme	microfluidic	1825:1836	arg1	devices					1838:1844	microfluidic devices	1825:1844	microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs	1825:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	50	theme	microfluidic	1825:1836	arg1	control					1855:1861	precise control	1847:1861	precise control of the environment	1847:1880	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	50	theme	microfluidic	1825:1836	arg1	parallelization					1913:1927	parallelization	1913:1927	parallelization for high throughput studies	1913:1955	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	50	theme	microfluidic	1825:1836	arg1	use					1971:1973	efficient use	1961:1973	efficient use of therapeutic drugs	1961:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	8	51	theme	migration	1344:1352	arg1	phenotype					1354:1362	migration phenotype	1344:1362	migration phenotype from mesenchymal or lobopodial to amoeboid	1344:1405	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	4	52	theme	disrupted	902:910	arg1	membrane					921:928	a disrupted basement membrane	900:928	a disrupted basement membrane	900:928	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	9	53	used	used	1623:1626	arg2	tools					1605:1609	software tools	1596:1609	software tools	1596:1609	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	9	53	used	used	1623:1626	arg2	set					1589:1591	a set	1587:1591	a set of software tools	1587:1609	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	9	53	used	used	1623:1626	arg2	platform					1574:1581	a robust microfluidic platform	1552:1581	a robust microfluidic platform	1552:1581	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	2	54	theme	composition	340:350	arg1	hydrogels					303:311	hydrogels	303:311	hydrogels of mixed collagen-Matrigel composition	303:350	In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments.
28166248	2	55	theme	mixed	316:320	arg1	composition					340:350	mixed collagen-Matrigel composition	316:350	mixed collagen-Matrigel composition	316:350	In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments.
28166248	3	56	theme	H1299	621:625	arg1	cells					654:658	H1299 lung adenocarcinoma cancer cells	621:658	H1299 lung adenocarcinoma cancer cells	621:658	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	3	57	theme	cancer	647:652	arg1	cells					654:658	H1299 lung adenocarcinoma cancer cells	621:658	H1299 lung adenocarcinoma cancer cells	621:658	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	8	58	theme	antibody-based	1290:1303	arg1	blockade					1314:1321	antibody-based integrin blockade	1290:1321	antibody-based integrin blockade	1290:1321	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	3	59	theme	lung	627:630	arg1	cells					654:658	H1299 lung adenocarcinoma cancer cells	621:658	H1299 lung adenocarcinoma cancer cells	621:658	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	9	60	theme	microfluidic	1561:1572	arg1	platform					1574:1581	a robust microfluidic platform	1552:1581	a robust microfluidic platform	1552:1581	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	6	61	dep	retaining	1156:1164	arg1	factor					1149:1154	factor	1149:1154	factor	1149:1154	At low concentration, Matrigel facilitates migration, most probably by providing a supportive and growth factor retaining environment.
28166248	6	61	dep	retaining	1156:1164	arg1	growth					1142:1147	growth	1142:1147	growth	1142:1147	At low concentration, Matrigel facilitates migration, most probably by providing a supportive and growth factor retaining environment.
28166248	7	62	theme	excessive	1246:1254	arg1	attachment					1256:1265	excessive attachment	1246:1265	excessive attachment	1246:1265	At high concentration, Matrigel slows down migration, possibly due excessive attachment.
28166248	2	63	theme	microfluidic	270:281	arg1	devices					283:289	microfluidic devices	270:289	microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition	270:350	In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments.
28166248	8	64	from	mesenchymal	1369:1379	arg1	change					1334:1339	a change	1332:1339	a change in migration phenotype from mesenchymal or lobopodial to amoeboid	1332:1405	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	8	64	from	mesenchymal	1369:1379	arg1	phenotype					1354:1362	migration phenotype	1344:1362	migration phenotype from mesenchymal or lobopodial to amoeboid	1344:1405	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	1	65	theme	mainstream	171:180	arg1	tools					182:186	mainstream tools	171:186	mainstream tools	171:186	Microfluidic devices are becoming mainstream tools to recapitulate in vitro the behavior of cells and tissues.
28166248	4	66	theme	cancer	738:743	arg1	plasticity					719:728	the plasticity	715:728	the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue	715:965	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	9	67	theme	software	1596:1603	arg1	tools					1605:1609	software tools	1596:1609	software tools	1596:1609	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	10	68	dep	devices	1838:1844	arg1	devices					1838:1844	microfluidic devices	1825:1844	microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs	1825:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	68	dep	devices	1838:1844	arg1	control					1855:1861	precise control	1847:1861	precise control of the environment	1847:1880	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	68	dep	devices	1838:1844	arg1	parallelization					1913:1927	parallelization	1913:1927	parallelization for high throughput studies	1913:1955	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	68	dep	devices	1838:1844	arg1	properties					1901:1910	excellent optical properties	1883:1910	excellent optical properties	1883:1910	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	10	68	dep	devices	1838:1844	arg1	use					1971:1973	efficient use	1961:1973	efficient use of therapeutic drugs	1961:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	0	69	theme	image	121:125	arg1	analysis					127:134	image analysis	121:134	image analysis	121:134	Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis.
28166248	4	70	from	lobopodial	820:829	arg1	matrices					852:859	collagen-Matrigel matrices	834:859	collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue	834:965	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	8	71	from	lobopodial	1384:1393	arg1	change					1334:1339	a change	1332:1339	a change in migration phenotype from mesenchymal or lobopodial to amoeboid	1332:1405	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	8	71	from	lobopodial	1384:1393	arg1	phenotype					1354:1362	migration phenotype	1344:1362	migration phenotype from mesenchymal or lobopodial to amoeboid	1344:1405	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	2	72	theme	cancer	412:417	arg1	microenvironments					428:444	different cancer invasion microenvironments	402:444	different cancer invasion microenvironments	402:444	In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments.
28166248	8	73	from	effect	1423:1428	arg1	dynamics					1458:1465	migration dynamics	1448:1465	migration dynamics	1448:1465	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	8	73	from	effect	1423:1428	arg1	regards					1471:1477	regards	1471:1477	regards to the structure of the matrix	1471:1508	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	5	74	theme	biphasic	1017:1024	arg1	role					1026:1029	a biphasic role	1015:1029	a biphasic role of Matrigel	1015:1041	Our quantification of migration speed confirms a biphasic role of Matrigel.
28166248	0	75	theme	cancer	38:43	arg1	migration					50:58	three-dimensional cancer cell migration	20:58	three-dimensional cancer cell migration	20:58	Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis.
28166248	9	76	theme	lung	1637:1640	arg1	migration					1654:1662	lung cancer cell migration	1637:1662	lung cancer cell migration	1637:1662	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	10	77	theme	optical	1893:1899	arg1	properties					1901:1910	excellent optical properties	1883:1910	excellent optical properties	1883:1910	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	4	78	theme	only	802:805	arg1	matrices					807:814	collagen only matrices	793:814	collagen only matrices	793:814	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	0	79	theme	migration	50:58	arg1	Characterization					0:15	Characterization	0:15	Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis.	0:135	Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis.
28166248	9	80	theme	cell	1649:1652	arg1	migration					1654:1662	lung cancer cell migration	1637:1662	lung cancer cell migration	1637:1662	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	10	81	theme	throughput	1938:1947	arg1	studies					1949:1955	high throughput studies	1933:1955	high throughput studies	1933:1955	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	3	82	theme	device	489:494	arg1	design					462:467	the design	458:467	the design of the microfluidic device	458:494	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	0	83	theme	mixed	63:67	arg1	scaffolds					87:95	mixed collagen-Matrigel scaffolds	63:95	mixed collagen-Matrigel scaffolds using microfluidics and image analysis	63:134	Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis.
28166248	10	84	theme	therapeutic	1978:1988	arg1	drugs					1990:1994	therapeutic drugs	1978:1994	therapeutic drugs	1978:1994	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	4	85	from	mesenchymal	778:788	arg1	turns					767:771	turns	767:771	turns	767:771	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	4	85	from	mesenchymal	778:788	arg1	matrices					807:814	collagen only matrices	793:814	collagen only matrices	793:814	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	10	86	theme	excellent	1883:1891	arg1	properties					1901:1910	excellent optical properties	1883:1910	excellent optical properties	1883:1910	This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
28166248	4	87	theme	collagen-Matrigel	834:850	arg1	matrices					852:859	collagen-Matrigel matrices	834:859	collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue	834:965	Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue.
28166248	2	88	theme	cancer	383:388	arg1	cells					390:394	lung cancer cells	378:394	lung cancer cells	378:394	In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments.
28166248	8	89	from	change	1438:1443	arg1	dynamics					1458:1465	migration dynamics	1448:1465	migration dynamics	1448:1465	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	8	89	from	change	1438:1443	arg1	regards					1471:1477	regards	1471:1477	regards to the structure of the matrix	1471:1508	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
28166248	9	90	theme	robust	1554:1559	arg1	platform					1574:1581	a robust microfluidic platform	1552:1581	a robust microfluidic platform	1552:1581	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	9	91	theme	experimental	1702:1713	arg1	conditions					1715:1724	experimental conditions	1702:1724	experimental conditions	1702:1724	In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions.
28166248	3	92	theme	quantitative	565:576	arg1	analysis					584:591	quantitative image analysis	565:591	quantitative image analysis	565:591	We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions.
28166248	8	93	from	regards	1471:1477	arg1	effect					1423:1428	the effect	1419:1428	the effect of this change in migration dynamics, in regards to the structure of the matrix	1419:1508	Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.
27473277	0	0	theme	micelles	89:96	arg1	delivery					44:51	intracellular delivery	30:51	intracellular delivery of drug-loaded hyaluronan polymeric micelles	30:96	Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles.
27473277	2	1	theme	up	515:516	arg1	design					488:493	design	488:493	design	488:493	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	2	1	theme	up	515:516	arg1	development					472:482	micelle development	464:482	micelle development	464:482	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	1	2	theme	administered	173:184	arg1	drugs					195:199	intravenously administered nonpolar drugs	159:199	intravenously administered nonpolar drugs	159:199	Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs.
27473277	2	3	theme	set	511:513	arg1	up					515:516	experimental set up	498:516	experimental set up	498:516	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	5	4	dep	in	848:849	arg1	vitro					851:855	vitro	851:855	vitro	851:855	Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component.
27473277	1	5	theme	nonpolar	186:193	arg1	drugs					195:199	intravenously administered nonpolar drugs	159:199	intravenously administered nonpolar drugs	159:199	Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs.
27473277	5	6	theme	serum	899:903	arg1	concentration					905:917	serum concentration	899:917	serum concentration	899:917	Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component.
27473277	0	7	from	Influence	0:8	arg1	delivery					44:51	intracellular delivery	30:51	intracellular delivery of drug-loaded hyaluronan polymeric micelles	30:96	Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles.
27473277	4	8	theme	loading	775:781	arg1	capacity					783:790	the loading capacity	771:790	the loading capacity	771:790	The composition of micelles affected mainly the loading capacity.
27473277	7	9	theme	intracellular	1232:1244	arg1	uptake					1246:1251	the in vitro intracellular uptake	1219:1251	the in vitro intracellular uptake of drugs loaded in nanocarriers	1219:1283	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	5	10	theme	destabilizing	968:980	arg1	albumin					932:938	serum albumin	926:938	serum albumin	926:938	Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component.
27473277	5	10	theme	destabilizing	968:980	arg1	component					982:990	the primary destabilizing component	956:990	the primary destabilizing component	956:990	Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component.
27473277	2	11	theme	serum	368:372	arg1	interaction					382:392	serum protein interaction	368:392	serum protein interaction	368:392	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	7	12	theme	micelle	1196:1202	arg1	ratio					1204:1208	serum albumin/polymeric micelle ratio	1172:1208	serum albumin/polymeric micelle ratio	1172:1208	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	5	13	theme	cell	857:860	arg1	uptake					862:867	the in vitro cell uptake	844:867	the in vitro cell uptake	844:867	Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component.
27473277	3	14	theme	hyaluronic	564:573	arg1	acid					575:578	amphiphilic hyaluronic acid	552:578	amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic)	552:637	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	2	15	theme	loaded	417:422	arg1	compound					424:431	loaded compound	417:431	loaded compound	417:431	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	7	16	theme	in	1223:1224	arg1	uptake					1246:1251	the in vitro intracellular uptake	1219:1251	the in vitro intracellular uptake of drugs loaded in nanocarriers	1219:1283	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	2	17	theme	experimental	498:509	arg1	up					515:516	experimental set up	498:516	experimental set up	498:516	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	7	18	theme	ratio	1204:1208	arg1	properties					1122:1131	the properties	1118:1131	the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio	1118:1208	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	0	19	theme	polymeric	79:87	arg1	micelles					89:96	drug-loaded hyaluronan polymeric micelles	56:96	drug-loaded hyaluronan polymeric micelles	56:96	Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles.
27473277	2	20	dep	development	472:482	arg1	the					460:462	the	460:462	the	460:462	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	7	21	theme	drugs	1256:1260	arg1	uptake					1246:1251	the in vitro intracellular uptake	1219:1251	the in vitro intracellular uptake of drugs loaded in nanocarriers	1219:1283	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	4	22	theme	micelles	746:753	arg1	composition					731:741	The composition	727:741	The composition of micelles	727:753	The composition of micelles affected mainly the loading capacity.
27473277	2	23	theme	micelle	464:470	arg1	development					472:482	micelle development	464:482	micelle development	464:482	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	1	24	theme	Polymeric	99:107	arg1	systems					147:153	attractive drug delivery systems	122:153	attractive drug delivery systems for intravenously administered nonpolar drugs	122:199	Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs.
27473277	1	24	theme	Polymeric	99:107	arg1	micelles					109:116	Polymeric micelles	99:116	Polymeric micelles	99:116	Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs.
27473277	3	25	dep	acids	625:629	arg1	oleic					632:636	oleic	632:636	oleic	632:636	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	0	26	theme	albumin	19:25	arg1	Influence					0:8	Influence	0:8	Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles.	0:97	Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles.
27473277	3	27	theme	short	593:597	arg1	acids					625:629	short (hexanoic) and long fatty acids	593:629	short (hexanoic) and long fatty acids (oleic)	593:637	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	0	28	theme	serum	13:17	arg1	albumin					19:25	serum albumin	13:25	serum albumin	13:25	Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles.
27473277	7	29	theme	chemical	1087:1094	arg1	structure					1096:1104	the chemical structure	1083:1104	the chemical structure of micelle	1083:1115	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	2	30	theme	physical	211:218	arg1	parameters					220:229	physical parameters	211:229	physical parameters like size, shape and loading capacity	211:267	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	3	31	with	loading	657:663	arg1	Nile					704:707	Nile red	704:711	Nile red	704:711	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	3	31	with	loading	657:663	arg1	curcumin					717:724	curcumin	717:724	curcumin	717:724	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	3	31	with	loading	657:663	arg1	models					696:701	two different hydrophobic models	670:701	two different hydrophobic models	670:701	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	3	32	dep	short	593:597	arg1	hexanoic					600:607	hexanoic	600:607	hexanoic	600:607	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	0	33	theme	intracellular	30:42	arg1	delivery					44:51	intracellular delivery	30:51	intracellular delivery of drug-loaded hyaluronan polymeric micelles	30:96	Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles.
27473277	3	34	theme	Polymeric	519:527	arg1	micelles					529:536	Polymeric micelles	519:536	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic)	519:637	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	3	35	theme	fatty	619:623	arg1	acids					625:629	short (hexanoic) and long fatty acids	593:629	short (hexanoic) and long fatty acids (oleic)	593:637	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	6	36	theme	micelle	1054:1060	arg1	concentration					1062:1074	polymeric micelle concentration	1044:1074	polymeric micelle concentration	1044:1074	This destabilization was found to be influenced by polymeric micelle concentration.
27473277	5	37	theme	encapsulated	798:809	arg1	compounds					811:819	Both encapsulated compounds	793:819	Both encapsulated compounds	793:819	Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component.
27473277	7	38	theme	serum	1172:1176	arg1	ratio					1204:1208	serum albumin/polymeric micelle ratio	1172:1208	serum albumin/polymeric micelle ratio	1172:1208	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	2	39	theme	loading	252:258	arg1	capacity					260:267	loading capacity	252:267	loading capacity	252:267	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	2	40	theme	compound	424:431	arg1	effects					357:363	the effects	353:363	the effects of serum protein interaction	353:392	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	2	40	theme	compound	424:431	arg1	characteristics					398:412	characteristics	398:412	characteristics of loaded compound	398:431	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	7	41	theme	loaded	1151:1156	arg1	substance					1158:1166	non-covalently loaded substance	1136:1166	non-covalently loaded substance	1136:1166	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	6	42	theme	polymeric	1044:1052	arg1	concentration					1062:1074	polymeric micelle concentration	1044:1074	polymeric micelle concentration	1044:1074	This destabilization was found to be influenced by polymeric micelle concentration.
27473277	1	43	theme	attractive	122:131	arg1	systems					147:153	attractive drug delivery systems	122:153	attractive drug delivery systems for intravenously administered nonpolar drugs	122:199	Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs.
27473277	1	43	theme	attractive	122:131	arg1	micelles					109:116	Polymeric micelles	99:116	Polymeric micelles	99:116	Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs.
27473277	7	44	theme	substance	1158:1166	arg1	properties					1122:1131	the properties	1118:1131	the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio	1118:1208	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	7	45	dep	in	1223:1224	arg1	vitro					1226:1230	vitro	1226:1230	vitro	1226:1230	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	3	46	theme	hydrophobic	684:694	arg1	Nile					704:707	Nile red	704:711	Nile red	704:711	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	3	46	theme	hydrophobic	684:694	arg1	curcumin					717:724	curcumin	717:724	curcumin	717:724	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	3	46	theme	hydrophobic	684:694	arg1	models					696:701	two different hydrophobic models	670:701	two different hydrophobic models	670:701	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	3	47	theme	long	614:617	arg1	acids					625:629	short (hexanoic) and long fatty acids	593:629	short (hexanoic) and long fatty acids (oleic)	593:637	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	1	48	theme	drug	133:136	arg1	systems					147:153	attractive drug delivery systems	122:153	attractive drug delivery systems for intravenously administered nonpolar drugs	122:199	Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs.
27473277	1	48	theme	drug	133:136	arg1	micelles					109:116	Polymeric micelles	99:116	Polymeric micelles	99:116	Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs.
27473277	7	49	theme	albumin/polymeric	1178:1194	arg1	ratio					1204:1208	serum albumin/polymeric micelle ratio	1172:1208	serum albumin/polymeric micelle ratio	1172:1208	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	5	50	theme	serum	926:930	arg1	component					982:990	the primary destabilizing component	956:990	the primary destabilizing component	956:990	Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component.
27473277	5	50	theme	serum	926:930	arg1	albumin					932:938	serum albumin	926:938	serum albumin	926:938	Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component.
27473277	7	51	theme	micelle	1109:1115	arg1	structure					1096:1104	the chemical structure	1083:1104	the chemical structure of micelle	1083:1115	Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers.
27473277	1	52	theme	delivery	138:145	arg1	systems					147:153	attractive drug delivery systems	122:153	attractive drug delivery systems for intravenously administered nonpolar drugs	122:199	Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs.
27473277	1	52	theme	delivery	138:145	arg1	micelles					109:116	Polymeric micelles	99:116	Polymeric micelles	99:116	Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs.
27473277	5	53	theme	primary	960:966	arg1	albumin					932:938	serum albumin	926:938	serum albumin	926:938	Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component.
27473277	5	53	theme	primary	960:966	arg1	component					982:990	the primary destabilizing component	956:990	the primary destabilizing component	956:990	Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component.
27473277	3	54	theme	amphiphilic	552:562	arg1	acid					575:578	amphiphilic hyaluronic acid	552:578	amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic)	552:637	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	0	55	theme	hyaluronan	68:77	arg1	micelles					89:96	drug-loaded hyaluronan polymeric micelles	56:96	drug-loaded hyaluronan polymeric micelles	56:96	Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles.
27473277	3	56	theme	different	674:682	arg1	Nile					704:707	Nile red	704:711	Nile red	704:711	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	3	56	theme	different	674:682	arg1	curcumin					717:724	curcumin	717:724	curcumin	717:724	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	3	56	theme	different	674:682	arg1	models					696:701	two different hydrophobic models	670:701	two different hydrophobic models	670:701	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	2	57	theme	interaction	382:392	arg1	effects					357:363	the effects	353:363	the effects of serum protein interaction	353:392	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	2	57	theme	interaction	382:392	arg1	characteristics					398:412	characteristics	398:412	characteristics of loaded compound	398:431	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
27473277	0	58	theme	drug-loaded	56:66	arg1	micelles					89:96	drug-loaded hyaluronan polymeric micelles	56:96	drug-loaded hyaluronan polymeric micelles	56:96	Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles.
27473277	3	59	theme	red	709:711	arg1	Nile					704:707	Nile red	704:711	Nile red	704:711	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	3	59	theme	red	709:711	arg1	models					696:701	two different hydrophobic models	670:701	two different hydrophobic models	670:701	Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin.
27473277	5	60	theme	in	848:849	arg1	uptake					862:867	the in vitro cell uptake	844:867	the in vitro cell uptake	844:867	Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component.
27473277	2	61	theme	protein	374:380	arg1	interaction					382:392	serum protein interaction	368:392	serum protein interaction	368:392	Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up.
24363237	6	0	theme	paper	1228:1232	arg1	FPase					1245:1249	FPase	1245:1249	FPase	1245:1249	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	6	0	theme	paper	1228:1232	arg1	cellulase					1234:1242	the highest filter paper cellulase	1209:1242	the highest filter paper cellulase (FPase)	1209:1250	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	5	1	theme	resulting	1087:1095	arg1	biomass					1097:1103	the resulting biomass	1083:1103	the resulting biomass	1083:1103	However, the high crystallinity (74%) of the resulting biomass was detrimental to microbial uptake and enzyme production.
24363237	1	2	theme	enzymatic	161:169	arg1	inputs					220:225	the most costly inputs	204:225	the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals	204:340	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	1	2	theme	enzymatic	161:169	arg1	one					197:199	one	197:199	one	197:199	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	1	2	theme	enzymatic	161:169	arg1	cocktail					171:178	The enzymatic cocktail	157:178	The enzymatic cocktail of cellulases	157:192	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	3	3	theme	filamentous	878:888	arg1	niger					909:913	the filamentous fungus Aspergillus niger	874:913	the filamentous fungus Aspergillus niger	874:913	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	4	theme	functional	678:687	arg1	groups					689:694	functional groups	678:694	functional groups (Fourier transform infrared spectroscopy)	678:736	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	6	5	theme	carboxymethyl	1253:1265	arg1	CMCase					1278:1283	CMCase	1278:1283	CMCase	1278:1283	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	6	5	theme	carboxymethyl	1253:1265	arg1	cellulase					1267:1275	carboxymethyl cellulase	1253:1275	carboxymethyl cellulase (CMCase)	1253:1284	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	7	6	theme	severe	1476:1481	arg1	conditions					1483:1492	severe conditions	1476:1492	severe conditions	1476:1492	The results showed that a suitable pretreatment for SCB to be used as a substrate for cellulase production should avoid severe conditions in order to preserve amorphous cellulose and to enhance the physical properties that assist microbial access.
24363237	4	7	theme	acid/alkali	960:970	arg1	pretreatment					972:983	The combined acid/alkali pretreatment	947:983	The combined acid/alkali pretreatment	947:983	The combined acid/alkali pretreatment resulted in a SCB with higher cellulose content (86.7%).
24363237	3	8	used	used	795:798	arg2	groups					689:694	functional groups	678:694	functional groups (Fourier transform infrared spectroscopy)	678:736	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	8	used	used	795:798	arg2	crystallinity					629:641	crystallinity	629:641	crystallinity (determined by X-ray diffraction)	629:675	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	8	used	used	795:798	arg2	characteristics					604:618	structural characteristics	593:618	structural characteristics	593:618	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	8	used	used	795:798	arg2	composition					577:587	Chemical composition	568:587	Chemical composition	568:587	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	8	used	used	795:798	arg2	microstructure					743:756	microstructure	743:756	microstructure (scanning electron microscopy)	743:787	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	4	9	theme	combined	951:958	arg1	pretreatment					972:983	The combined acid/alkali pretreatment	947:983	The combined acid/alkali pretreatment	947:983	The combined acid/alkali pretreatment resulted in a SCB with higher cellulose content (86.7%).
24363237	5	10	theme	enzyme	1145:1150	arg1	production					1152:1161	enzyme production	1145:1161	enzyme production	1145:1161	However, the high crystallinity (74%) of the resulting biomass was detrimental to microbial uptake and enzyme production.
24363237	3	11	theme	niger	909:913	arg1	strain					864:869	a strain	862:869	a strain of the filamentous fungus Aspergillus niger under solid-state fermentation	862:944	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	1	12	theme	biochemical	267:277	arg1	route					279:283	the biochemical route	263:283	the biochemical route for biomass conversion into biofuels and other chemicals	263:340	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	0	13	theme	cellulase	104:112	arg1	production					114:123	cellulase production	104:123	cellulase production under solid-state fermentation	104:154	Use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation.
24363237	2	14	theme	dilute	384:389	arg1	acid					391:394	dilute acid	384:394	dilute acid	384:394	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	2	15	theme	cellulase	480:488	arg1	production					490:499	cellulase production	480:499	cellulase production	480:499	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	4	16	theme	cellulose	1015:1023	arg1	content					1025:1031	higher cellulose content	1008:1031	higher cellulose content (86.7%)	1008:1039	The combined acid/alkali pretreatment resulted in a SCB with higher cellulose content (86.7%).
24363237	4	16	theme	cellulose	1015:1023	arg1	%					1038:1038	86.7%	1034:1038	86.7%	1034:1038	The combined acid/alkali pretreatment resulted in a SCB with higher cellulose content (86.7%).
24363237	2	17	from	influence	353:361	arg1	SCB					466:468	SCB	466:468	SCB	466:468	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	2	17	from	influence	353:361	arg1	bagasse					457:463	sugarcane bagasse	447:463	sugarcane bagasse (SCB) used for cellulase production	447:499	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	1	18	theme	cellulases	183:192	arg1	inputs					220:225	the most costly inputs	204:225	the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals	204:340	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	1	18	theme	cellulases	183:192	arg1	one					197:199	one	197:199	one	197:199	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	1	18	theme	cellulases	183:192	arg1	cocktail					171:178	The enzymatic cocktail	157:178	The enzymatic cocktail of cellulases	157:192	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	2	19	theme	pretreatments	430:442	arg1	influence					353:361	the influence	349:361	the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production	349:499	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	3	20	theme	infrared	715:722	arg1	spectroscopy					724:735	infrared spectroscopy	715:735	infrared spectroscopy	715:735	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	21	theme	Aspergillus	897:907	arg1	niger					909:913	the filamentous fungus Aspergillus niger	874:913	the filamentous fungus Aspergillus niger	874:913	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	2	22	theme	water	377:381	arg1	influence					353:361	the influence	349:361	the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production	349:499	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	2	23	theme	acid/alkali	418:428	arg1	pretreatments					430:442	combined acid/alkali pretreatments	409:442	combined acid/alkali pretreatments	409:442	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	1	24	theme	route	279:283	arg1	viability					250:258	the economic viability	237:258	the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals	237:340	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	5	25	theme	high	1055:1058	arg1	detrimental					1109:1119	detrimental	1109:1119	detrimental	1109:1119	However, the high crystallinity (74%) of the resulting biomass was detrimental to microbial uptake and enzyme production.
24363237	5	25	theme	high	1055:1058	arg1	crystallinity					1060:1072	the high crystallinity	1051:1072	the high crystallinity (74%) of the resulting biomass	1051:1103	However, the high crystallinity (74%) of the resulting biomass was detrimental to microbial uptake and enzyme production.
24363237	5	25	theme	high	1055:1058	arg1	%					1077:1077	74%	1075:1077	74%	1075:1077	However, the high crystallinity (74%) of the resulting biomass was detrimental to microbial uptake and enzyme production.
24363237	7	26	theme	amorphous	1515:1523	arg1	cellulose					1525:1533	amorphous cellulose	1515:1533	amorphous cellulose	1515:1533	The results showed that a suitable pretreatment for SCB to be used as a substrate for cellulase production should avoid severe conditions in order to preserve amorphous cellulose and to enhance the physical properties that assist microbial access.
24363237	2	27	theme	hot	373:375	arg1	water					377:381	liquid hot water	366:381	liquid hot water	366:381	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	2	28	theme	combined	409:416	arg1	pretreatments					430:442	combined acid/alkali pretreatments	409:442	combined acid/alkali pretreatments	409:442	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	3	29	dep	groups	689:694	arg1	transform					705:713	transform	705:713	transform infrared spectroscopy	705:735	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	2	30	theme	spectroscopic	530:542	arg1	techniques					556:565	spectroscopic and imaging techniques	530:565	techniques	556:565	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	3	31	dep	filamentous	878:888	arg1	fungus					890:895	fungus	890:895	fungus	890:895	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	32	theme	SCB	813:815	arg1	pretreatments					817:829	SCB pretreatments	813:829	SCB pretreatments	813:829	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	7	33	theme	microbial	1586:1594	arg1	access					1596:1601	microbial access	1586:1601	microbial access	1586:1601	The results showed that a suitable pretreatment for SCB to be used as a substrate for cellulase production should avoid severe conditions in order to preserve amorphous cellulose and to enhance the physical properties that assist microbial access.
24363237	0	34	theme	spectroscopic	7:19	arg1	techniques					33:42	spectroscopic and imaging techniques	7:42	techniques	33:42	Use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation.
24363237	1	35	theme	biomass	289:295	arg1	conversion					297:306	biomass conversion	289:306	biomass conversion into biofuels and other chemicals	289:340	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	3	36	theme	solid-state	921:931	arg1	fermentation					933:944	solid-state fermentation	921:944	solid-state fermentation	921:944	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	2	37	theme	alkali	397:402	arg1	influence					353:361	the influence	349:361	the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production	349:499	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	0	38	theme	techniques	33:42	arg1	Use					0:2	Use	0:2	Use of spectroscopic and imaging techniques	0:42	Use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation.
24363237	6	39	theme	xylanase	1291:1298	arg1	activities					1300:1309	xylanase activities	1291:1309	xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively)	1291:1353	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	7	40	theme	cellulase	1442:1450	arg1	production					1452:1461	cellulase production	1442:1461	cellulase production	1442:1461	The results showed that a suitable pretreatment for SCB to be used as a substrate for cellulase production should avoid severe conditions in order to preserve amorphous cellulose and to enhance the physical properties that assist microbial access.
24363237	6	41	theme	filter	1221:1226	arg1	FPase					1245:1249	FPase	1245:1249	FPase	1245:1249	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	6	41	theme	filter	1221:1226	arg1	cellulase					1234:1242	the highest filter paper cellulase	1209:1242	the highest filter paper cellulase (FPase)	1209:1250	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	0	42	theme	imaging	25:31	arg1	techniques					33:42	spectroscopic and imaging techniques	7:42	techniques	33:42	Use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation.
24363237	5	43	theme	microbial	1124:1132	arg1	uptake					1134:1139	microbial uptake	1124:1139	microbial uptake	1124:1139	However, the high crystallinity (74%) of the resulting biomass was detrimental to microbial uptake and enzyme production.
24363237	0	44	theme	solid-state	131:141	arg1	fermentation					143:154	solid-state fermentation	131:154	solid-state fermentation	131:154	Use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation.
24363237	3	45	theme	structural	593:602	arg1	microstructure					743:756	microstructure	743:756	microstructure (scanning electron microscopy)	743:787	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	45	theme	structural	593:602	arg1	crystallinity					629:641	crystallinity	629:641	crystallinity (determined by X-ray diffraction)	629:675	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	45	theme	structural	593:602	arg1	groups					689:694	functional groups	678:694	functional groups (Fourier transform infrared spectroscopy)	678:736	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	45	theme	structural	593:602	arg1	characteristics					604:618	structural characteristics	593:618	structural characteristics	593:618	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	7	46	theme	physical	1554:1561	arg1	properties					1563:1572	the physical properties	1550:1572	the physical properties that assist microbial access	1550:1601	The results showed that a suitable pretreatment for SCB to be used as a substrate for cellulase production should avoid severe conditions in order to preserve amorphous cellulose and to enhance the physical properties that assist microbial access.
24363237	1	47	theme	costly	213:218	arg1	inputs					220:225	the most costly inputs	204:225	the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals	204:340	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	2	48	theme	sugarcane	447:455	arg1	SCB					466:468	SCB	466:468	SCB	466:468	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	2	48	theme	sugarcane	447:455	arg1	bagasse					457:463	sugarcane bagasse	447:463	sugarcane bagasse (SCB) used for cellulase production	447:499	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	6	49	theme	U	1332:1332	arg1	-1					1336:1337	-1	1336:1337	-1	1336:1337	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	6	49	theme	U	1332:1332	arg1	g					1334:1334	0.4, 14.9, and 26.1 U g	1312:1334	0.4, 14.9, and 26.1 U g(-1)	1312:1338	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	3	50	theme	Chemical	568:575	arg1	composition					577:587	Chemical composition	568:587	Chemical composition	568:587	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	4	51	with	SCB	999:1001	arg1	content					1025:1031	higher cellulose content	1008:1031	higher cellulose content (86.7%)	1008:1039	The combined acid/alkali pretreatment resulted in a SCB with higher cellulose content (86.7%).
24363237	4	51	with	SCB	999:1001	arg1	%					1038:1038	86.7%	1034:1038	86.7%	1034:1038	The combined acid/alkali pretreatment resulted in a SCB with higher cellulose content (86.7%).
24363237	6	52	dep	activities	1300:1309	arg1	-1					1336:1337	-1	1336:1337	-1	1336:1337	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	6	52	dep	activities	1300:1309	arg1	g					1334:1334	0.4, 14.9, and 26.1 U g	1312:1334	0.4, 14.9, and 26.1 U g(-1)	1312:1338	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	3	53	theme	scanning	759:766	arg1	microstructure					743:756	microstructure	743:756	microstructure (scanning electron microscopy)	743:787	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	53	theme	scanning	759:766	arg1	microscopy					777:786	scanning electron microscopy	759:786	scanning electron microscopy	759:786	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	1	54	theme	other	326:330	arg1	chemicals					332:340	other chemicals	326:340	other chemicals	326:340	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	2	55	theme	imaging	548:554	arg1	techniques					556:565	spectroscopic and imaging techniques	530:565	techniques	556:565	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	6	56	theme	highest	1213:1219	arg1	FPase					1245:1249	FPase	1245:1249	FPase	1245:1249	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	6	56	theme	highest	1213:1219	arg1	cellulase					1234:1242	the highest filter paper cellulase	1209:1242	the highest filter paper cellulase (FPase)	1209:1250	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	1	57	theme	inputs	220:225	arg1	inputs					220:225	the most costly inputs	204:225	the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals	204:340	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	1	57	theme	inputs	220:225	arg1	one					197:199	one	197:199	one	197:199	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	1	57	theme	inputs	220:225	arg1	cocktail					171:178	The enzymatic cocktail	157:178	The enzymatic cocktail of cellulases	157:192	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
24363237	3	58	theme	electron	768:775	arg1	microstructure					743:756	microstructure	743:756	microstructure (scanning electron microscopy)	743:787	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	58	theme	electron	768:775	arg1	microscopy					777:786	scanning electron microscopy	759:786	scanning electron microscopy	759:786	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	3	59	theme	X-ray	658:662	arg1	diffraction					664:674	X-ray diffraction	658:674	X-ray diffraction	658:674	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	6	60	theme	hot	1191:1193	arg1	water					1195:1199	liquid hot water	1184:1199	liquid hot water	1184:1199	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	3	61	theme	enzymatic	836:844	arg1	biosynthesis					846:857	enzymatic biosynthesis	836:857	enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation	836:944	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	0	62	theme	sugarcane	67:75	arg1	bagasse					77:83	pretreated sugarcane bagasse	56:83	pretreated sugarcane bagasse	56:83	Use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation.
24363237	0	62	theme	sugarcane	67:75	arg1	substrate					90:98	a substrate	88:98	a substrate for cellulase production under solid-state fermentation	88:154	Use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation.
24363237	2	63	theme	acid	391:394	arg1	influence					353:361	the influence	349:361	the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production	349:499	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	6	64	theme	liquid	1184:1189	arg1	water					1195:1199	liquid hot water	1184:1199	liquid hot water	1184:1199	SCB pretreated with liquid hot water yielded the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase), and xylanase activities (0.4, 14.9, and 26.1 U g(-1), respectively).
24363237	2	65	theme	liquid	366:371	arg1	water					377:381	liquid hot water	366:381	liquid hot water	366:381	Here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (SCB) used for cellulase production was investigated by means of spectroscopic and imaging techniques.
24363237	7	66	theme	suitable	1382:1389	arg1	pretreatment					1391:1402	a suitable pretreatment	1380:1402	a suitable pretreatment for SCB to be used as a substrate for cellulase production	1380:1461	The results showed that a suitable pretreatment for SCB to be used as a substrate for cellulase production should avoid severe conditions in order to preserve amorphous cellulose and to enhance the physical properties that assist microbial access.
24363237	0	67	theme	pretreated	56:65	arg1	bagasse					77:83	pretreated sugarcane bagasse	56:83	pretreated sugarcane bagasse	56:83	Use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation.
24363237	0	67	theme	pretreated	56:65	arg1	substrate					90:98	a substrate	88:98	a substrate for cellulase production under solid-state fermentation	88:154	Use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation.
24363237	5	68	theme	biomass	1097:1103	arg1	detrimental					1109:1119	detrimental	1109:1119	detrimental	1109:1119	However, the high crystallinity (74%) of the resulting biomass was detrimental to microbial uptake and enzyme production.
24363237	5	68	theme	biomass	1097:1103	arg1	crystallinity					1060:1072	the high crystallinity	1051:1072	the high crystallinity (74%) of the resulting biomass	1051:1103	However, the high crystallinity (74%) of the resulting biomass was detrimental to microbial uptake and enzyme production.
24363237	5	68	theme	biomass	1097:1103	arg1	%					1077:1077	74%	1075:1077	74%	1075:1077	However, the high crystallinity (74%) of the resulting biomass was detrimental to microbial uptake and enzyme production.
24363237	4	69	theme	higher	1008:1013	arg1	content					1025:1031	higher cellulose content	1008:1031	higher cellulose content (86.7%)	1008:1039	The combined acid/alkali pretreatment resulted in a SCB with higher cellulose content (86.7%).
24363237	4	69	theme	higher	1008:1013	arg1	%					1038:1038	86.7%	1034:1038	86.7%	1034:1038	The combined acid/alkali pretreatment resulted in a SCB with higher cellulose content (86.7%).
24363237	3	70	dep	crystallinity	629:641	arg1	determined					644:653	determined	644:653	determined by X-ray diffraction	644:674	Chemical composition and structural characteristics, such as crystallinity (determined by X-ray diffraction), functional groups (Fourier transform infrared spectroscopy), and microstructure (scanning electron microscopy), were used to correlate SCB pretreatments with enzymatic biosynthesis by a strain of the filamentous fungus Aspergillus niger under solid-state fermentation.
24363237	1	71	theme	economic	241:248	arg1	viability					250:258	the economic viability	237:258	the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals	237:340	The enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals.
25595634	10	0	theme	freezing	1553:1560	arg1	curve					1573:1577	The freezing controlled curve	1549:1577	The freezing controlled curve used in this study	1549:1596	The freezing controlled curve used in this study allowed standardization of the cryopreservation technique.
25595634	3	1	with	semen	520:524	arg1	curve					552:556	a controlled cooling curve	531:556	a controlled cooling curve	531:556	The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve.
25595634	4	2	dep	solutions	580:588	arg1	glycerol					616:623	3% glycerol	613:623	3% glycerol	613:623	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	2	dep	solutions	580:588	arg1	combination					633:643	the combination	629:643	the combination of these two cryoprotectants	629:672	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	2	dep	solutions	580:588	arg1	dimethylformamide					594:610	5% dimethylformamide	591:610	5% dimethylformamide	591:610	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	2	dep	solutions	580:588	arg1	solutions					580:588	Three cryoprotectant solutions	559:588	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants)	559:673	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	3	3	theme	semen	520:524	arg1	cryopreservation					494:509	cryopreservation	494:509	cryopreservation of swine semen with a controlled cooling curve	494:556	The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve.
25595634	1	4	from	spermatozoa	152:162	arg1	damage					169:174	the damage	165:174	the damage caused by cryopreservation	165:201	In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization.
25595634	1	4	from	spermatozoa	152:162	arg1	severe					211:216	severe	211:216	severe	211:216	In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization.
25595634	1	5	theme	reduced	248:254	arg1	potential					256:264	reduced potential	248:264	reduced potential for fertilization	248:282	In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization.
25595634	0	6	theme	coconut	105:111	arg1	water					113:117	powdered coconut water	96:117	powdered coconut water	96:117	Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose.
25595634	9	7	theme	sperm	1525:1529	arg1	cryopreservation					1531:1546	swine sperm cryopreservation	1519:1546	swine sperm cryopreservation	1519:1546	Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation.
25595634	8	8	theme	water	1329:1333	arg1	solutions					1209:1217	the solutions	1205:1217	the solutions of lactose, trehalose and powdered coconut water associated with glycerol	1205:1291	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	8	8	theme	water	1329:1333	arg1	solution					1309:1316	the solution	1305:1316	the solution of coconut water containing dimethylformamide	1305:1362	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	4	9	theme	3	613:613	arg1	%					614:614	%	614:614	%	614:614	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	0	10	theme	powdered	96:103	arg1	water					113:117	powdered coconut water	96:117	powdered coconut water	96:117	Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose.
25595634	4	11	used	used	680:683	arg2	combination					633:643	the combination	629:643	the combination of these two cryoprotectants	629:672	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	11	used	used	680:683	arg2	solutions					580:588	Three cryoprotectant solutions	559:588	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants)	559:673	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	11	used	used	680:683	arg2	glycerol					616:623	3% glycerol	613:623	3% glycerol	613:623	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	11	used	used	680:683	arg2	dimethylformamide					594:610	5% dimethylformamide	591:610	5% dimethylformamide	591:610	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	10	12	theme	technique	1646:1654	arg1	standardization					1606:1620	standardization	1606:1620	standardization of the cryopreservation technique	1606:1654	The freezing controlled curve used in this study allowed standardization of the cryopreservation technique.
25595634	8	13	theme	coconut	1321:1327	arg1	water					1329:1333	coconut water	1321:1333	coconut water containing dimethylformamide	1321:1362	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	6	14	theme	plasma	955:960	arg1	integrity					971:979	plasma membrane integrity	955:979	plasma membrane integrity	955:979	After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity.
25595634	7	15	theme	swine	1175:1179	arg1	sperm					1181:1185	swine sperm	1175:1185	swine sperm	1175:1185	Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm.
25595634	8	16	theme	coconut	1254:1260	arg1	water					1262:1266	powdered coconut water	1245:1266	powdered coconut water	1245:1266	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	3	17	theme	cooling	544:550	arg1	curve					552:556	a controlled cooling curve	531:556	a controlled cooling curve	531:556	The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve.
25595634	8	18	theme	trehalose	1231:1239	arg1	solutions					1209:1217	the solutions	1205:1217	the solutions of lactose, trehalose and powdered coconut water associated with glycerol	1205:1291	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	8	18	theme	trehalose	1231:1239	arg1	solution					1309:1316	the solution	1305:1316	the solution of coconut water containing dimethylformamide	1305:1362	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	7	19	with	Cryopreservation	1005:1020	arg1	curve					1042:1046	the controlled curve	1027:1046	the controlled curve using an automated system	1027:1072	Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm.
25595634	4	20	with	association	688:698	arg1	media					716:720	three base media	705:720	three base media (powdered coconut water, lactose and trehalose)	705:768	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	20	with	association	688:698	arg1	lactose					747:753	lactose	747:753	lactose	747:753	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	20	with	association	688:698	arg1	water					740:744	powdered coconut water	723:744	powdered coconut water	723:744	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	20	with	association	688:698	arg1	trehalose					759:767	trehalose	759:767	trehalose	759:767	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	3	21	theme	study	416:420	arg1	objective					398:406	The objective	394:406	The objective of this study	394:420	The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve.
25595634	9	22	theme	swine	1519:1523	arg1	cryopreservation					1531:1546	swine sperm cryopreservation	1519:1546	swine sperm cryopreservation	1519:1546	Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation.
25595634	4	23	theme	%	614:614	arg1	glycerol					616:623	3% glycerol	613:623	3% glycerol	613:623	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	23	theme	%	614:614	arg1	solutions					580:588	Three cryoprotectant solutions	559:588	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants)	559:673	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	24	theme	cryoprotectants	658:672	arg1	glycerol					616:623	3% glycerol	613:623	3% glycerol	613:623	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	24	theme	cryoprotectants	658:672	arg1	combination					633:643	the combination	629:643	the combination of these two cryoprotectants	629:672	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	24	theme	cryoprotectants	658:672	arg1	dimethylformamide					594:610	5% dimethylformamide	591:610	5% dimethylformamide	591:610	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	24	theme	cryoprotectants	658:672	arg1	solutions					580:588	Three cryoprotectant solutions	559:588	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants)	559:673	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	8	25	theme	powdered	1245:1252	arg1	water					1262:1266	powdered coconut water	1245:1266	powdered coconut water	1245:1266	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	7	26	theme	satisfactory	1081:1092	arg1	results					1094:1100	satisfactory results	1081:1100	satisfactory results	1081:1100	Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm.
25595634	2	27	theme	extender	313:320	arg1	composition					322:332	the freezing extender composition	300:332	the freezing extender composition	300:332	Adjustments in the freezing extender composition may be an important alternative to increase its efficiency.
25595634	0	28	theme	sperm	25:29	arg1	Cryopreservation					0:15	Cryopreservation	0:15	Cryopreservation of boar sperm comparing different cryoprotectants	0:65	Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose.
25595634	4	29	theme	coconut	732:738	arg1	media					716:720	three base media	705:720	three base media (powdered coconut water, lactose and trehalose)	705:768	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	29	theme	coconut	732:738	arg1	water					740:744	powdered coconut water	723:744	powdered coconut water	723:744	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	7	30	theme	automated	1057:1065	arg1	system					1067:1072	an automated system	1054:1072	an automated system	1054:1072	Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm.
25595634	2	31	theme	freezing	304:311	arg1	composition					322:332	the freezing extender composition	300:332	the freezing extender composition	300:332	Adjustments in the freezing extender composition may be an important alternative to increase its efficiency.
25595634	3	32	theme	cryoprotectant	462:475	arg1	solutions					477:485	different cryoprotectant solutions	452:485	different cryoprotectant solutions	452:485	The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve.
25595634	0	33	theme	boar	20:23	arg1	sperm					25:29	boar sperm	20:29	boar sperm comparing different cryoprotectants	20:65	Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose.
25595634	4	34	theme	5	591:591	arg1	%					592:592	%	592:592	%	592:592	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	8	35	theme	higher	1375:1380	arg1	quality					1382:1388	higher quality	1375:1388	higher quality of sperm	1375:1397	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	3	36	theme	solutions	477:485	arg1	efficiency					438:447	the efficiency	434:447	the efficiency of different cryoprotectant solutions	434:485	The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve.
25595634	0	37	theme	different	41:49	arg1	cryoprotectants					51:65	different cryoprotectants	41:65	different cryoprotectants	41:65	Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose.
25595634	4	38	theme	base	711:714	arg1	media					716:720	three base media	705:720	three base media (powdered coconut water, lactose and trehalose)	705:768	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	38	theme	base	711:714	arg1	lactose					747:753	lactose	747:753	lactose	747:753	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	38	theme	base	711:714	arg1	water					740:744	powdered coconut water	723:744	powdered coconut water	723:744	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	38	theme	base	711:714	arg1	trehalose					759:767	trehalose	759:767	trehalose	759:767	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	8	39	theme	sperm	1393:1397	arg1	quality					1382:1388	higher quality	1375:1388	higher quality of sperm	1375:1397	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	9	40	theme	Powdered	1432:1439	arg1	water					1449:1453	Powdered coconut water	1432:1453	Powdered coconut water associated with dimethylformamide	1432:1487	Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation.
25595634	4	41	theme	%	592:592	arg1	solutions					580:588	Three cryoprotectant solutions	559:588	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants)	559:673	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	41	theme	%	592:592	arg1	dimethylformamide					594:610	5% dimethylformamide	591:610	5% dimethylformamide	591:610	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	3	42	theme	different	452:460	arg1	solutions					477:485	different cryoprotectant solutions	452:485	different cryoprotectant solutions	452:485	The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve.
25595634	8	43	theme	lactose	1222:1228	arg1	solutions					1209:1217	the solutions	1205:1217	the solutions of lactose, trehalose and powdered coconut water associated with glycerol	1205:1291	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	8	43	theme	lactose	1222:1228	arg1	solution					1309:1316	the solution	1305:1316	the solution of coconut water containing dimethylformamide	1305:1362	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	8	44	contain	containing	1335:1344	arg2	dimethylformamide					1346:1362	dimethylformamide	1346:1362	dimethylformamide	1346:1362	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	8	44	contain	containing	1335:1344	arg1	water					1329:1333	coconut water	1321:1333	coconut water containing dimethylformamide	1321:1362	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	7	45	theme	controlled	1031:1040	arg1	curve					1042:1046	the controlled curve	1027:1046	the controlled curve using an automated system	1027:1072	Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm.
25595634	4	46	theme	cryoprotectant	565:578	arg1	solutions					580:588	Three cryoprotectant solutions	559:588	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants)	559:673	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	46	theme	cryoprotectant	565:578	arg1	combination					633:643	the combination	629:643	the combination of these two cryoprotectants	629:672	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	46	theme	cryoprotectant	565:578	arg1	dimethylformamide					594:610	5% dimethylformamide	591:610	5% dimethylformamide	591:610	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	46	theme	cryoprotectant	565:578	arg1	glycerol					616:623	3% glycerol	613:623	3% glycerol	613:623	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	1	47	from	severe	211:216	arg1	spermatozoa					152:162	swine spermatozoa	146:162	swine spermatozoa	146:162	In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization.
25595634	2	48	theme	important	344:352	arg1	Adjustments					285:295	Adjustments	285:295	Adjustments in the freezing extender composition	285:332	Adjustments in the freezing extender composition may be an important alternative to increase its efficiency.
25595634	2	48	theme	important	344:352	arg1	alternative					354:364	an important alternative	341:364	an important alternative to increase its efficiency	341:391	Adjustments in the freezing extender composition may be an important alternative to increase its efficiency.
25595634	10	49	theme	cryopreservation	1629:1644	arg1	technique					1646:1654	the cryopreservation technique	1625:1654	the cryopreservation technique	1625:1654	The freezing controlled curve used in this study allowed standardization of the cryopreservation technique.
25595634	6	50	theme	sperm	920:924	arg1	motility					926:933	total sperm motility	914:933	total sperm motility	914:933	After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity.
25595634	3	51	theme	swine	514:518	arg1	semen					520:524	swine semen	514:524	swine semen with a controlled cooling curve	514:556	The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve.
25595634	6	52	theme	total	914:918	arg1	motility					926:933	total sperm motility	914:933	total sperm motility	914:933	After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity.
25595634	8	53	theme	other	1415:1419	arg1	solutions					1421:1429	the other solutions	1411:1429	the other solutions	1411:1429	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	4	54	theme	powdered	723:730	arg1	media					716:720	three base media	705:720	three base media (powdered coconut water, lactose and trehalose)	705:768	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	54	theme	powdered	723:730	arg1	water					740:744	powdered coconut water	723:744	powdered coconut water	723:744	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	55	dep	media	716:720	arg1	trehalose					759:767	trehalose	759:767	trehalose	759:767	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	55	dep	media	716:720	arg1	lactose					747:753	lactose	747:753	lactose	747:753	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	55	dep	media	716:720	arg1	water					740:744	powdered coconut water	723:744	powdered coconut water	723:744	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	4	55	dep	media	716:720	arg1	media					716:720	three base media	705:720	three base media (powdered coconut water, lactose and trehalose)	705:768	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	9	56	theme	coconut	1441:1447	arg1	water					1449:1453	Powdered coconut water	1432:1453	Powdered coconut water associated with dimethylformamide	1432:1487	Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation.
25595634	10	57	theme	controlled	1562:1571	arg1	curve					1573:1577	The freezing controlled curve	1549:1577	The freezing controlled curve used in this study	1549:1596	The freezing controlled curve used in this study allowed standardization of the cryopreservation technique.
25595634	1	58	theme	swine	146:150	arg1	spermatozoa					152:162	swine spermatozoa	146:162	swine spermatozoa	146:162	In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization.
25595634	1	59	theme	other	223:227	arg1	species					229:235	other species	223:235	other species	223:235	In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization.
25595634	5	60	theme	controlled-rate	840:854	arg1	TK-3000					865:871	TK-3000	865:871	TK-3000	865:871	The semen was frozen using a controlled-rate freezer (TK-3000).
25595634	5	60	theme	controlled-rate	840:854	arg1	freezer					856:862	a controlled-rate freezer	838:862	a controlled-rate freezer (TK-3000)	838:872	The semen was frozen using a controlled-rate freezer (TK-3000).
25595634	8	61	theme	water	1262:1266	arg1	solutions					1209:1217	the solutions	1205:1217	the solutions of lactose, trehalose and powdered coconut water associated with glycerol	1205:1291	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	8	61	theme	water	1262:1266	arg1	solution					1309:1316	the solution	1305:1316	the solution of coconut water containing dimethylformamide	1305:1362	With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions.
25595634	9	62	theme	new	1502:1504	arg1	solution					1506:1513	a new solution	1500:1513	a new solution for swine sperm cryopreservation	1500:1546	Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation.
25595634	2	63	from	Adjustments	285:295	arg1	composition					322:332	the freezing extender composition	300:332	the freezing extender composition	300:332	Adjustments in the freezing extender composition may be an important alternative to increase its efficiency.
25595634	6	64	theme	membrane	962:969	arg1	integrity					971:979	plasma membrane integrity	955:979	plasma membrane integrity	955:979	After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity.
25595634	4	65	theme	different	789:797	arg1	treatments					799:808	nine different treatments	784:808	nine different treatments	784:808	Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments.
25595634	3	66	theme	controlled	533:542	arg1	curve					552:556	a controlled cooling curve	531:556	a controlled cooling curve	531:556	The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve.
25595634	6	67	theme	acrosome	985:992	arg1	integrity					994:1002	acrosome integrity	985:1002	acrosome integrity	985:1002	After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity.
25082625	13	0	theme	hyaline	2462:2468	arg1	cartilage					2470:2478	the normal hip hyaline cartilage	2447:2478	the normal hip hyaline cartilage	2447:2478	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	4	1	theme	cartilage	740:748	arg1	profile					706:712	the T1ρ MRI profile	694:712	the T1ρ MRI profile of the normal hip hyaline cartilage	694:748	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	3	2	from	hip	424:426	arg1	Knowledge					360:368	Knowledge	360:368	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip	360:426	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip is important for establishing a baseline against which comparisons can be made to experimental and clinical arthritic subjects.
25082625	11	3	theme	anterolateral-most	1972:1989	arg1	region					1991:1996	the anterolateral-most region	1968:1996	the anterolateral-most region	1968:1996	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	11	4	theme	slices	1924:1929	arg1	combination					1899:1909	a selective combination	1887:1909	a selective combination of zones and slices	1887:1929	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	8	5	theme	relaxation	1386:1395	arg1	values					1397:1402	the T1ρ relaxation values	1378:1402	the T1ρ relaxation values	1378:1402	RESULTS There was variation in the T1ρ relaxation values depending on zone (anterior to posterior) and slice (medial to lateral).
25082625	12	6	theme	hip	2184:2186	arg1	cartilage					2167:2175	normal hyaline cartilage	2152:2175	normal hyaline cartilage of the hip	2152:2186	CONCLUSIONS The T1ρ profile of normal hyaline cartilage of the hip is not uniform with the topographic differences identified suggesting regional variations in PG concentration.
25082625	6	7	dep	posterosuperior	1081:1095	arg1	3					1078:1078	3	1078:1078	3	1078:1078	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	7	8	theme	T1ρ	1222:1224	arg1	value					1237:1241	the mean T1ρ relaxation value	1213:1241	the mean T1ρ relaxation value	1213:1241	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	7	8	theme	T1ρ	1222:1224	arg1	parameter					1246:1254	a parameter	1244:1254	a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration	1244:1344	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	12	9	theme	PG	2281:2282	arg1	concentration					2284:2296	PG concentration	2281:2296	PG concentration	2281:2296	CONCLUSIONS The T1ρ profile of normal hyaline cartilage of the hip is not uniform with the topographic differences identified suggesting regional variations in PG concentration.
25082625	14	10	contain	has	2523:2525	arg1	MRI					2519:2521	MRI	2519:2521	MRI	2519:2521	Furthermore, it demonstrates that T1ρ MRI has the ability to detect even subtle, microscopic local differences in hyaline cartilage composition.
25082625	14	10	contain	has	2523:2525	arg2	ability					2531:2537	the ability to detect even subtle, microscopic local differences in hyaline cartilage composition	2527:2623	the ability to detect even subtle, microscopic local differences in hyaline cartilage composition	2527:2623	Furthermore, it demonstrates that T1ρ MRI has the ability to detect even subtle, microscopic local differences in hyaline cartilage composition.
25082625	7	11	theme	sagittal	1183:1190	arg1	slice					1192:1196	each sagittal slice	1178:1196	each sagittal slice	1178:1196	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	9	12	theme	posterior	1601:1609	arg1	quadrants					1611:1619	the combined posterior quadrants	1588:1619	the combined posterior quadrants (Zones 3 and 4)	1588:1635	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	9	12	theme	posterior	1601:1609	arg1	Zones					1622:1626	Zones 3 and 4	1622:1634	Zones 3 and 4	1622:1634	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	13	13	theme	normal	2451:2456	arg1	cartilage					2470:2478	the normal hip hyaline cartilage	2447:2478	the normal hip hyaline cartilage	2447:2478	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	4	14	theme	hip	728:730	arg1	cartilage					740:748	the normal hip hyaline cartilage	717:748	the normal hip hyaline cartilage	717:748	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	13	15	theme	cartilage	2470:2478	arg1	region					2437:2442	the more anterolateral region	2414:2442	the more anterolateral region of the normal hip hyaline cartilage	2414:2478	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	7	16	theme	each	1164:1167	arg1	zone					1169:1172	each zone	1164:1172	each zone	1164:1172	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	1	17	theme	hyaline	156:162	arg1	cartilage					164:172	hyaline cartilage	156:172	hyaline cartilage	156:172	BACKGROUND T1ρ MRI is an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage.
25082625	6	18	dep	zones	1036:1040	arg1	anterosuperior					1061:1074	(1) anterior; (2) anterosuperior	1043:1074	(1) anterior; (2) anterosuperior	1043:1074	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	6	18	dep	zones	1036:1040	arg1	zones					1036:1040	four zones	1031:1040	four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior	1031:1118	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	6	18	dep	zones	1036:1040	arg1	4					1103:1103	4	1103:1103	4	1103:1103	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	6	18	dep	zones	1036:1040	arg1	posterior					1110:1118	posterior	1110:1118	posterior	1110:1118	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	4	19	theme	diagnostic	583:592	arg1	study					594:598	this diagnostic study	578:598	this diagnostic study	578:598	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	2	20	theme	normative	184:192	arg1	values					198:203	normative T1ρ values	184:203	normative T1ρ values	184:203	However, normative T1ρ values have not been established for the weightbearing cartilage of the hip, and it is not known whether it is uniform or whether there is topographic variation.
25082625	6	21	theme	anterior	1047:1054	arg1	anterosuperior					1061:1074	(1) anterior; (2) anterosuperior	1043:1074	(1) anterior; (2) anterosuperior	1043:1074	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	6	21	theme	anterior	1047:1054	arg1	zones					1036:1040	four zones	1031:1040	four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior	1031:1118	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	10	22	theme	p	1809:1809	arg1	slices					1801:1806	the sagittal slices	1788:1806	the sagittal slices (p = 0.038)	1788:1818	There was a difference between the T1ρ relaxation values of the sagittal slices (p = 0.038), most pronounced anteriorly in Zone 1 (26.6 msec, p = 0.001).
25082625	10	22	theme	p	1809:1809	arg1	0.038					1813:1817	p = 0.038	1809:1817	p = 0.038	1809:1817	There was a difference between the T1ρ relaxation values of the sagittal slices (p = 0.038), most pronounced anteriorly in Zone 1 (26.6 msec, p = 0.001).
25082625	4	23	theme	MRI	627:629	arg1	values					631:636	the T1ρ MRI values	619:636	(1) the T1ρ MRI values of hyaline cartilage of the normal hip	615:675	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	15	24	theme	growing	2809:2815	arg1	repertoire					2817:2826	the growing repertoire	2805:2826	the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage	2805:2899	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	15	24	theme	growing	2809:2815	arg1	techniques					2857:2866	advanced, biochemical MRI techniques	2831:2866	advanced, biochemical MRI techniques for evaluating hyaline cartilage	2831:2899	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	13	25	theme	T1ρ	2342:2344	arg1	values					2357:2362	lower T1ρ relaxation values	2336:2362	lower T1ρ relaxation values	2336:2362	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	7	26	theme	full	1129:1132	arg1	region					1134:1139	the full region	1125:1139	the full region of interest	1125:1151	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	10	27	theme	slices	1801:1806	arg1	values					1778:1783	the T1ρ relaxation values	1759:1783	the T1ρ relaxation values of the sagittal slices (p = 0.038)	1759:1818	There was a difference between the T1ρ relaxation values of the sagittal slices (p = 0.038), most pronounced anteriorly in Zone 1 (26.6 msec, p = 0.001).
25082625	13	28	theme	anterolateral	2423:2435	arg1	region					2437:2442	the more anterolateral region	2414:2442	the more anterolateral region of the normal hip hyaline cartilage	2414:2478	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	5	29	theme	hip	895:897	arg1	MRI					879:881	1.5-T T1ρ MRI	869:881	1.5-T T1ρ MRI of a single hip	869:897	METHODS Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years) prospectively underwent 1.5-T T1ρ MRI of a single hip.
25082625	1	30	theme	imaging	96:102	arg1	MRI					86:88	BACKGROUND T1ρ MRI	71:88	BACKGROUND T1ρ MRI	71:88	BACKGROUND T1ρ MRI is an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage.
25082625	1	30	theme	imaging	96:102	arg1	technique					104:112	an imaging technique	93:112	an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage	93:172	BACKGROUND T1ρ MRI is an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage.
25082625	15	31	theme	noninvasive	2725:2735	arg1	surrogate					2737:2745	a noninvasive surrogate or biomarker	2723:2758	surrogate	2737:2745	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	11	32	theme	weightbearing	2033:2045	arg1	portion					2047:2053	the weightbearing portion	2029:2053	the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001)	2029:2118	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	3	33	theme	arthritic	536:544	arg1	subjects					546:553	experimental and clinical arthritic subjects	510:553	experimental and clinical arthritic subjects	510:553	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip is important for establishing a baseline against which comparisons can be made to experimental and clinical arthritic subjects.
25082625	13	34	theme	PG	2393:2394	arg1	concentrations					2396:2409	inherently greater PG concentrations	2374:2409	inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage	2374:2478	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	1	35	theme	sensitive	114:122	arg1	MRI					86:88	BACKGROUND T1ρ MRI	71:88	BACKGROUND T1ρ MRI	71:88	BACKGROUND T1ρ MRI is an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage.
25082625	1	35	theme	sensitive	114:122	arg1	technique					104:112	an imaging technique	93:112	an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage	93:172	BACKGROUND T1ρ MRI is an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage.
25082625	7	36	theme	PG	1329:1330	arg1	concentration					1332:1344	PG concentration	1329:1344	PG concentration	1329:1344	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	11	37	theme	hip	2062:2064	arg1	portion					2047:2053	the weightbearing portion	2029:2053	the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001)	2029:2118	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	0	38	theme	normal	51:56	arg1	uniform					62:68	the normal hip uniform	47:68	the normal hip uniform	47:68	Is the T1ρ MRI profile of hyaline cartilage in the normal hip uniform?
25082625	13	39	theme	relaxation	2346:2355	arg1	values					2357:2362	lower T1ρ relaxation values	2336:2362	lower T1ρ relaxation values	2336:2362	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	1	40	theme	proteoglycan	127:138	arg1	content					145:151	proteoglycan (PG) content	127:151	proteoglycan (PG) content of hyaline cartilage	127:172	BACKGROUND T1ρ MRI is an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage.
25082625	13	41	theme	values	2357:2362	arg1	determination					2319:2331	determination	2319:2331	determination of lower T1ρ relaxation values	2319:2362	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	11	42	theme	T1ρ	1954:1956	arg1	values					1958:1963	lower mean T1ρ values	1943:1963	lower mean T1ρ values	1943:1963	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	4	43	theme	hip	673:675	arg1	cartilage					649:657	hyaline cartilage	641:657	hyaline cartilage of the normal hip	641:675	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	12	44	theme	cartilage	2167:2175	arg1	profile					2141:2147	The T1ρ profile	2133:2147	The T1ρ profile of normal hyaline cartilage of the hip	2133:2186	CONCLUSIONS The T1ρ profile of normal hyaline cartilage of the hip is not uniform with the topographic differences identified suggesting regional variations in PG concentration.
25082625	15	45	theme	clinical	2691:2698	arg1	research					2700:2707	clinical research	2691:2707	clinical research	2691:2707	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	10	46	theme	relaxation	1767:1776	arg1	values					1778:1783	the T1ρ relaxation values	1759:1783	the T1ρ relaxation values of the sagittal slices (p = 0.038)	1759:1818	There was a difference between the T1ρ relaxation values of the sagittal slices (p = 0.038), most pronounced anteriorly in Zone 1 (26.6 msec, p = 0.001).
25082625	3	47	theme	hyaline	392:398	arg1	cartilage					400:408	hyaline cartilage	392:408	hyaline cartilage	392:408	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip is important for establishing a baseline against which comparisons can be made to experimental and clinical arthritic subjects.
25082625	5	48	dep	volunteers	792:801	arg1	women					818:822	three women	812:822	three women	812:822	METHODS Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years) prospectively underwent 1.5-T T1ρ MRI of a single hip.
25082625	5	48	dep	volunteers	792:801	arg1	years					838:842	35 years	835:842	35 years	835:842	METHODS Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years) prospectively underwent 1.5-T T1ρ MRI of a single hip.
25082625	5	48	dep	volunteers	792:801	arg1	men					807:809	11 men	804:809	11 men	804:809	METHODS Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years) prospectively underwent 1.5-T T1ρ MRI of a single hip.
25082625	14	49	theme	local	2574:2578	arg1	differences					2580:2590	even subtle, microscopic local differences	2549:2590	differences	2580:2590	Furthermore, it demonstrates that T1ρ MRI has the ability to detect even subtle, microscopic local differences in hyaline cartilage composition.
25082625	6	50	theme	hyaline	918:924	arg1	bilayer					936:942	The weightbearing hyaline cartilage bilayer	900:942	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head	900:977	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	0	51	from	profile	15:21	arg1	uniform					62:68	the normal hip uniform	47:68	the normal hip uniform	47:68	Is the T1ρ MRI profile of hyaline cartilage in the normal hip uniform?
25082625	8	52	dep	slice	1450:1454	arg1	medial					1457:1462	medial	1457:1462	medial	1457:1462	RESULTS There was variation in the T1ρ relaxation values depending on zone (anterior to posterior) and slice (medial to lateral).
25082625	4	53	theme	cartilage	649:657	arg1	values					631:636	the T1ρ MRI values	619:636	(1) the T1ρ MRI values of hyaline cartilage of the normal hip	615:675	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	12	54	from	variations	2267:2276	arg1	concentration					2284:2296	PG concentration	2281:2296	PG concentration	2281:2296	CONCLUSIONS The T1ρ profile of normal hyaline cartilage of the hip is not uniform with the topographic differences identified suggesting regional variations in PG concentration.
25082625	13	55	theme	lower	2336:2340	arg1	values					2357:2362	lower T1ρ relaxation values	2336:2362	lower T1ρ relaxation values	2336:2362	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	4	56	dep	values	631:636	arg1	1					616:616	1	616:616	1	616:616	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	0	57	theme	T1ρ	7:9	arg1	profile					15:21	the T1ρ MRI profile	3:21	the T1ρ MRI profile of hyaline cartilage in the normal hip uniform	3:68	Is the T1ρ MRI profile of hyaline cartilage in the normal hip uniform?
25082625	1	58	theme	T1ρ	82:84	arg1	MRI					86:88	BACKGROUND T1ρ MRI	71:88	BACKGROUND T1ρ MRI	71:88	BACKGROUND T1ρ MRI is an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage.
25082625	1	58	theme	T1ρ	82:84	arg1	technique					104:112	an imaging technique	93:112	an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage	93:172	BACKGROUND T1ρ MRI is an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage.
25082625	10	59	theme	T1ρ	1763:1765	arg1	values					1778:1783	the T1ρ relaxation values	1759:1783	the T1ρ relaxation values of the sagittal slices (p = 0.038)	1759:1818	There was a difference between the T1ρ relaxation values of the sagittal slices (p = 0.038), most pronounced anteriorly in Zone 1 (26.6 msec, p = 0.001).
25082625	9	60	theme	PG	1710:1711	arg1	concentration					1713:1725	higher PG concentration	1703:1725	higher PG concentration	1703:1725	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	4	61	theme	T1ρ	698:700	arg1	profile					706:712	the T1ρ MRI profile	694:712	the T1ρ MRI profile of the normal hip hyaline cartilage	694:748	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	3	62	theme	T1ρ	377:379	arg1	profile					381:387	the T1ρ profile	373:387	the T1ρ profile of hyaline cartilage in the normal hip	373:426	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip is important for establishing a baseline against which comparisons can be made to experimental and clinical arthritic subjects.
25082625	7	63	theme	PG	1283:1284	arg1	content					1286:1292	PG content	1283:1292	PG content	1283:1292	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	5	64	theme	T1ρ	875:877	arg1	MRI					879:881	1.5-T T1ρ MRI	869:881	1.5-T T1ρ MRI of a single hip	869:897	METHODS Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years) prospectively underwent 1.5-T T1ρ MRI of a single hip.
25082625	3	65	from	profile	381:387	arg1	hip					424:426	the normal hip	413:426	the normal hip	413:426	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip is important for establishing a baseline against which comparisons can be made to experimental and clinical arthritic subjects.
25082625	0	66	theme	hyaline	26:32	arg1	cartilage					34:42	hyaline cartilage	26:42	hyaline cartilage	26:42	Is the T1ρ MRI profile of hyaline cartilage in the normal hip uniform?
25082625	4	67	dep	QUESTIONS/PURPOSES	556:573	arg1	uniform					753:759	uniform	753:759	is uniform	750:759	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	4	67	dep	QUESTIONS/PURPOSES	556:573	arg1	whether					686:692	whether	686:692	whether	686:692	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	4	67	dep	QUESTIONS/PURPOSES	556:573	arg1	determined					604:613	determined	604:613	determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip	604:675	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	15	68	theme	techniques	2857:2866	arg1	repertoire					2817:2826	the growing repertoire	2805:2826	the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage	2805:2899	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	15	68	theme	techniques	2857:2866	arg1	techniques					2857:2866	advanced, biochemical MRI techniques	2831:2866	advanced, biochemical MRI techniques for evaluating hyaline cartilage	2831:2899	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	6	69	theme	head	974:977	arg1	bilayer					936:942	The weightbearing hyaline cartilage bilayer	900:942	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head	900:977	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	9	70	theme	relaxation	1545:1554	arg1	lower					1568:1572	lower	1568:1572	lower	1568:1572	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	9	70	theme	relaxation	1545:1554	arg1	values					1556:1561	the T1ρ relaxation values	1537:1561	the T1ρ relaxation values	1537:1561	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	3	71	theme	cartilage	400:408	arg1	profile					381:387	the T1ρ profile	373:387	the T1ρ profile of hyaline cartilage in the normal hip	373:426	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip is important for establishing a baseline against which comparisons can be made to experimental and clinical arthritic subjects.
25082625	9	72	dep	=	1682:1682	arg1	msec					1643:1646	30.4 msec	1638:1646	30.4 msec	1638:1646	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	9	72	dep	=	1682:1682	arg1	msec					1660:1663	32.2 msec	1655:1663	32.2 msec	1655:1663	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	11	73	dep	hip	2062:2064	arg1	=					2111:2111	=	2111:2111	=	2111:2111	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	12	74	theme	hyaline	2159:2165	arg1	cartilage					2167:2175	normal hyaline cartilage	2152:2175	normal hyaline cartilage of the hip	2152:2186	CONCLUSIONS The T1ρ profile of normal hyaline cartilage of the hip is not uniform with the topographic differences identified suggesting regional variations in PG concentration.
25082625	11	75	theme	selective	1889:1897	arg1	combination					1899:1909	a selective combination	1887:1909	a selective combination of zones and slices	1887:1929	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	3	76	from	Knowledge	360:368	arg1	hip					424:426	the normal hip	413:426	the normal hip	413:426	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip is important for establishing a baseline against which comparisons can be made to experimental and clinical arthritic subjects.
25082625	13	77	theme	hip	2458:2460	arg1	cartilage					2470:2478	the normal hip hyaline cartilage	2447:2478	the normal hip hyaline cartilage	2447:2478	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	15	78	theme	advanced	2831:2838	arg1	techniques					2857:2866	advanced, biochemical MRI techniques	2831:2866	advanced, biochemical MRI techniques for evaluating hyaline cartilage	2831:2899	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	6	79	dep	anterosuperior	1061:1074	arg1	2					1058:1058	2	1058:1058	2	1058:1058	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	6	79	dep	anterosuperior	1061:1074	arg1	1					1044:1044	1	1044:1044	1	1044:1044	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	6	79	dep	anterosuperior	1061:1074	arg1	posterosuperior					1081:1095	(3) posterosuperior	1077:1095	(3) posterosuperior	1077:1095	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	7	80	theme	relaxation	1226:1235	arg1	value					1237:1241	the mean T1ρ relaxation value	1213:1241	the mean T1ρ relaxation value	1213:1241	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	7	80	theme	relaxation	1226:1235	arg1	parameter					1246:1254	a parameter	1244:1254	a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration	1244:1344	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	4	81	theme	hyaline	732:738	arg1	cartilage					740:748	the normal hip hyaline cartilage	717:748	the normal hip hyaline cartilage	717:748	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	2	82	theme	weightbearing	239:251	arg1	cartilage					253:261	the weightbearing cartilage	235:261	the weightbearing cartilage of the hip	235:272	However, normative T1ρ values have not been established for the weightbearing cartilage of the hip, and it is not known whether it is uniform or whether there is topographic variation.
25082625	7	83	theme	mean	1217:1220	arg1	value					1237:1241	the mean T1ρ relaxation value	1213:1241	the mean T1ρ relaxation value	1213:1241	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	7	83	theme	mean	1217:1220	arg1	parameter					1246:1254	a parameter	1244:1254	a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration	1244:1344	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	9	84	theme	combined	1592:1599	arg1	quadrants					1611:1619	the combined posterior quadrants	1588:1619	the combined posterior quadrants (Zones 3 and 4)	1588:1635	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	9	84	theme	combined	1592:1599	arg1	Zones					1622:1626	Zones 3 and 4	1622:1634	Zones 3 and 4	1622:1634	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	8	85	theme	T1ρ	1382:1384	arg1	values					1397:1402	the T1ρ relaxation values	1378:1402	the T1ρ relaxation values	1378:1402	RESULTS There was variation in the T1ρ relaxation values depending on zone (anterior to posterior) and slice (medial to lateral).
25082625	14	86	theme	subtle	2554:2559	arg1	differences					2580:2590	even subtle, microscopic local differences	2549:2590	differences	2580:2590	Furthermore, it demonstrates that T1ρ MRI has the ability to detect even subtle, microscopic local differences in hyaline cartilage composition.
25082625	14	87	theme	cartilage	2603:2611	arg1	composition					2613:2623	hyaline cartilage composition	2595:2623	hyaline cartilage composition	2595:2623	Furthermore, it demonstrates that T1ρ MRI has the ability to detect even subtle, microscopic local differences in hyaline cartilage composition.
25082625	2	88	theme	hip	270:272	arg1	cartilage					253:261	the weightbearing cartilage	235:261	the weightbearing cartilage of the hip	235:272	However, normative T1ρ values have not been established for the weightbearing cartilage of the hip, and it is not known whether it is uniform or whether there is topographic variation.
25082625	4	89	theme	normal	721:726	arg1	cartilage					740:748	the normal hip hyaline cartilage	717:748	the normal hip hyaline cartilage	717:748	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	6	90	theme	sagittal	996:1003	arg1	images					1005:1010	sagittal images	996:1010	sagittal images	996:1010	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	10	91	theme	sagittal	1792:1799	arg1	slices					1801:1806	the sagittal slices	1788:1806	the sagittal slices (p = 0.038)	1788:1818	There was a difference between the T1ρ relaxation values of the sagittal slices (p = 0.038), most pronounced anteriorly in Zone 1 (26.6 msec, p = 0.001).
25082625	10	91	theme	sagittal	1792:1799	arg1	0.038					1813:1817	p = 0.038	1809:1817	p = 0.038	1809:1817	There was a difference between the T1ρ relaxation values of the sagittal slices (p = 0.038), most pronounced anteriorly in Zone 1 (26.6 msec, p = 0.001).
25082625	8	92	from	variation	1365:1373	arg1	values					1397:1402	the T1ρ relaxation values	1378:1402	the T1ρ relaxation values	1378:1402	RESULTS There was variation in the T1ρ relaxation values depending on zone (anterior to posterior) and slice (medial to lateral).
25082625	10	93	theme	pronounced	1826:1835	arg1	difference					1740:1749	a difference	1738:1749	a difference	1738:1749	There was a difference between the T1ρ relaxation values of the sagittal slices (p = 0.038), most pronounced anteriorly in Zone 1 (26.6 msec, p = 0.001).
25082625	5	94	theme	asymptomatic	779:790	arg1	volunteers					792:801	Fourteen asymptomatic volunteers	770:801	Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years)	770:843	METHODS Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years) prospectively underwent 1.5-T T1ρ MRI of a single hip.
25082625	15	95	dep	advanced	2831:2838	arg1	biochemical					2841:2851	biochemical	2841:2851	biochemical	2841:2851	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	3	96	theme	experimental	510:521	arg1	subjects					546:553	experimental and clinical arthritic subjects	510:553	experimental and clinical arthritic subjects	510:553	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip is important for establishing a baseline against which comparisons can be made to experimental and clinical arthritic subjects.
25082625	1	97	theme	cartilage	164:172	arg1	content					145:151	proteoglycan (PG) content	127:151	proteoglycan (PG) content of hyaline cartilage	127:172	BACKGROUND T1ρ MRI is an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage.
25082625	12	98	theme	regional	2258:2265	arg1	variations					2267:2276	regional variations	2258:2276	regional variations in PG concentration	2258:2296	CONCLUSIONS The T1ρ profile of normal hyaline cartilage of the hip is not uniform with the topographic differences identified suggesting regional variations in PG concentration.
25082625	10	99	theme	=	1811:1811	arg1	slices					1801:1806	the sagittal slices	1788:1806	the sagittal slices (p = 0.038)	1788:1818	There was a difference between the T1ρ relaxation values of the sagittal slices (p = 0.038), most pronounced anteriorly in Zone 1 (26.6 msec, p = 0.001).
25082625	10	99	theme	=	1811:1811	arg1	0.038					1813:1817	p = 0.038	1809:1817	p = 0.038	1809:1817	There was a difference between the T1ρ relaxation values of the sagittal slices (p = 0.038), most pronounced anteriorly in Zone 1 (26.6 msec, p = 0.001).
25082625	10	100	dep	=	1872:1872	arg1	msec					1864:1867	26.6 msec	1859:1867	26.6 msec	1859:1867	There was a difference between the T1ρ relaxation values of the sagittal slices (p = 0.038), most pronounced anteriorly in Zone 1 (26.6 msec, p = 0.001).
25082625	7	101	theme	interest	1144:1151	arg1	region					1134:1139	the full region	1125:1139	the full region of interest	1125:1151	For the full region of interest and within each zone and each sagittal slice, we calculated the mean T1ρ relaxation value, a parameter that indirectly quantifies PG content, where T1ρ is inversely related to PG concentration.
25082625	8	102	dep	anterior	1423:1430	arg1	to					1432:1433	to	1432:1433	to	1432:1433	RESULTS There was variation in the T1ρ relaxation values depending on zone (anterior to posterior) and slice (medial to lateral).
25082625	8	102	dep	anterior	1423:1430	arg1	posterior					1435:1443	posterior	1435:1443	posterior	1435:1443	RESULTS There was variation in the T1ρ relaxation values depending on zone (anterior to posterior) and slice (medial to lateral).
25082625	4	103	theme	T1ρ	623:625	arg1	values					631:636	the T1ρ MRI values	619:636	(1) the T1ρ MRI values of hyaline cartilage of the normal hip	615:675	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	5	104	theme	single	888:893	arg1	hip					895:897	a single hip	886:897	a single hip	886:897	METHODS Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years) prospectively underwent 1.5-T T1ρ MRI of a single hip.
25082625	12	105	theme	T1ρ	2137:2139	arg1	profile					2141:2147	The T1ρ profile	2133:2147	The T1ρ profile of normal hyaline cartilage of the hip	2133:2186	CONCLUSIONS The T1ρ profile of normal hyaline cartilage of the hip is not uniform with the topographic differences identified suggesting regional variations in PG concentration.
25082625	12	106	theme	topographic	2212:2222	arg1	differences					2224:2234	the topographic differences	2208:2234	the topographic differences identified suggesting regional variations in PG concentration	2208:2296	CONCLUSIONS The T1ρ profile of normal hyaline cartilage of the hip is not uniform with the topographic differences identified suggesting regional variations in PG concentration.
25082625	15	107	contain	has	2641:2643	arg1	technique					2631:2639	This technique	2626:2639	This technique	2626:2639	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	15	107	contain	has	2641:2643	arg2	potential					2649:2657	the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health	2645:2778	the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health	2645:2778	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	8	108	dep	zone	1417:1420	arg1	anterior					1423:1430	anterior	1423:1430	anterior	1423:1430	RESULTS There was variation in the T1ρ relaxation values depending on zone (anterior to posterior) and slice (medial to lateral).
25082625	3	109	theme	clinical	527:534	arg1	subjects					546:553	experimental and clinical arthritic subjects	510:553	experimental and clinical arthritic subjects	510:553	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip is important for establishing a baseline against which comparisons can be made to experimental and clinical arthritic subjects.
25082625	13	110	theme	greater	2385:2391	arg1	concentrations					2396:2409	inherently greater PG concentrations	2374:2409	inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage	2374:2478	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	12	111	dep	CONCLUSIONS	2121:2131	arg1	uniform					2195:2201	uniform	2195:2201	is not uniform with the topographic differences identified suggesting regional variations in PG concentration	2188:2296	CONCLUSIONS The T1ρ profile of normal hyaline cartilage of the hip is not uniform with the topographic differences identified suggesting regional variations in PG concentration.
25082625	0	112	theme	hip	58:60	arg1	uniform					62:68	the normal hip uniform	47:68	the normal hip uniform	47:68	Is the T1ρ MRI profile of hyaline cartilage in the normal hip uniform?
25082625	11	113	theme	portion	2047:2053	arg1	remainder					2016:2024	the remainder	2012:2024	the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001)	2012:2118	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	11	114	theme	mean	1949:1952	arg1	values					1958:1963	lower mean T1ρ values	1943:1963	lower mean T1ρ values	1943:1963	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	14	115	theme	hyaline	2595:2601	arg1	composition					2613:2623	hyaline cartilage composition	2595:2623	hyaline cartilage composition	2595:2623	Furthermore, it demonstrates that T1ρ MRI has the ability to detect even subtle, microscopic local differences in hyaline cartilage composition.
25082625	9	116	dep	lower	1568:1572	arg1	=					1682:1682	=	1682:1682	=	1682:1682	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	5	117	theme	mean	825:828	arg1	age					830:832	mean age	825:832	mean age	825:832	METHODS Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years) prospectively underwent 1.5-T T1ρ MRI of a single hip.
25082625	11	118	dep	=	2111:2111	arg1	msec					2072:2075	28.6 msec	2067:2075	28.6 msec	2067:2075	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	11	118	dep	=	2111:2111	arg1	msec					2089:2092	32.2 msec	2084:2092	32.2 msec	2084:2092	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	9	119	theme	anterior	1501:1508	arg1	Zones					1521:1525	Zones 1 and 2	1521:1533	Zones	1521:1525	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	9	119	theme	anterior	1501:1508	arg1	2					1533:1533	2	1533:1533	2	1533:1533	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	9	119	theme	anterior	1501:1508	arg1	quadrants					1510:1518	the most anterior quadrants	1492:1518	the most anterior quadrants (Zones 1 and 2)	1492:1534	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	4	120	theme	normal	666:671	arg1	hip					673:675	the normal hip	662:675	the normal hip	662:675	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	12	121	theme	normal	2152:2157	arg1	cartilage					2167:2175	normal hyaline cartilage	2152:2175	normal hyaline cartilage of the hip	2152:2186	CONCLUSIONS The T1ρ profile of normal hyaline cartilage of the hip is not uniform with the topographic differences identified suggesting regional variations in PG concentration.
25082625	5	122	dep	men	807:809	arg1	age					830:832	mean age	825:832	mean age	825:832	METHODS Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years) prospectively underwent 1.5-T T1ρ MRI of a single hip.
25082625	6	123	theme	cartilage	926:934	arg1	bilayer					936:942	The weightbearing hyaline cartilage bilayer	900:942	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head	900:977	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	14	124	theme	microscopic	2562:2572	arg1	differences					2580:2590	even subtle, microscopic local differences	2549:2590	differences	2580:2590	Furthermore, it demonstrates that T1ρ MRI has the ability to detect even subtle, microscopic local differences in hyaline cartilage composition.
25082625	8	125	dep	RESULTS	1347:1353	arg1	was					1361:1363	was	1361:1363	was variation in the T1ρ relaxation values depending on zone (anterior to posterior) and slice (medial to lateral)	1361:1474	RESULTS There was variation in the T1ρ relaxation values depending on zone (anterior to posterior) and slice (medial to lateral).
25082625	6	126	theme	weightbearing	904:916	arg1	bilayer					936:942	The weightbearing hyaline cartilage bilayer	900:942	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head	900:977	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	4	127	theme	hyaline	641:647	arg1	cartilage					649:657	hyaline cartilage	641:657	hyaline cartilage of the normal hip	641:675	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	0	128	theme	MRI	11:13	arg1	profile					15:21	the T1ρ MRI profile	3:21	the T1ρ MRI profile of hyaline cartilage in the normal hip uniform	3:68	Is the T1ρ MRI profile of hyaline cartilage in the normal hip uniform?
25082625	8	129	dep	medial	1457:1462	arg1	to					1464:1465	to	1464:1465	to	1464:1465	RESULTS There was variation in the T1ρ relaxation values depending on zone (anterior to posterior) and slice (medial to lateral).
25082625	8	129	dep	medial	1457:1462	arg1	lateral					1467:1473	lateral	1467:1473	lateral	1467:1473	RESULTS There was variation in the T1ρ relaxation values depending on zone (anterior to posterior) and slice (medial to lateral).
25082625	1	130	theme	BACKGROUND	71:80	arg1	MRI					86:88	BACKGROUND T1ρ MRI	71:88	BACKGROUND T1ρ MRI	71:88	BACKGROUND T1ρ MRI is an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage.
25082625	1	130	theme	BACKGROUND	71:80	arg1	technique					104:112	an imaging technique	93:112	an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage	93:172	BACKGROUND T1ρ MRI is an imaging technique sensitive to proteoglycan (PG) content of hyaline cartilage.
25082625	2	131	theme	T1ρ	194:196	arg1	values					198:203	normative T1ρ values	184:203	normative T1ρ values	184:203	However, normative T1ρ values have not been established for the weightbearing cartilage of the hip, and it is not known whether it is uniform or whether there is topographic variation.
25082625	9	132	theme	higher	1703:1708	arg1	concentration					1713:1725	higher PG concentration	1703:1725	higher PG concentration	1703:1725	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	5	133	dep	METHODS	762:768	arg1	underwent					859:867	underwent	859:867	underwent 1.5-T T1ρ MRI of a single hip	859:897	METHODS Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years) prospectively underwent 1.5-T T1ρ MRI of a single hip.
25082625	2	134	theme	topographic	337:347	arg1	variation					349:357	topographic variation	337:357	topographic variation	337:357	However, normative T1ρ values have not been established for the weightbearing cartilage of the hip, and it is not known whether it is uniform or whether there is topographic variation.
25082625	5	135	theme	1.5-T	869:873	arg1	MRI					879:881	1.5-T T1ρ MRI	869:881	1.5-T T1ρ MRI of a single hip	869:897	METHODS Fourteen asymptomatic volunteers (11 men, three women; mean age, 35 years) prospectively underwent 1.5-T T1ρ MRI of a single hip.
25082625	3	136	theme	profile	381:387	arg1	Knowledge					360:368	Knowledge	360:368	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip	360:426	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip is important for establishing a baseline against which comparisons can be made to experimental and clinical arthritic subjects.
25082625	0	137	theme	cartilage	34:42	arg1	profile					15:21	the T1ρ MRI profile	3:21	the T1ρ MRI profile of hyaline cartilage in the normal hip uniform	3:68	Is the T1ρ MRI profile of hyaline cartilage in the normal hip uniform?
25082625	15	138	theme	health	2773:2778	arg1	biomarker					2750:2758	a noninvasive surrogate or biomarker	2723:2758	biomarker	2750:2758	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	15	138	theme	health	2773:2778	arg1	surrogate					2737:2745	a noninvasive surrogate or biomarker	2723:2758	surrogate	2737:2745	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	15	139	theme	hyaline	2883:2889	arg1	cartilage					2891:2899	hyaline cartilage	2883:2899	hyaline cartilage	2883:2899	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	9	140	theme	T1ρ	1541:1543	arg1	lower					1568:1572	lower	1568:1572	lower	1568:1572	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	9	140	theme	T1ρ	1541:1543	arg1	values					1556:1561	the T1ρ relaxation values	1537:1561	the T1ρ relaxation values	1537:1561	When combining the most anterior quadrants (Zones 1 and 2), the T1ρ relaxation values were lower than those in the combined posterior quadrants (Zones 3 and 4) (30.4 msec versus 32.2 msec, respectively; p = 0.002), reflecting higher PG concentration.
25082625	4	141	theme	MRI	702:704	arg1	profile					706:712	the T1ρ MRI profile	694:712	the T1ρ MRI profile of the normal hip hyaline cartilage	694:748	QUESTIONS/PURPOSES In this diagnostic study, we determined (1) the T1ρ MRI values of hyaline cartilage of the normal hip; and (2) whether the T1ρ MRI profile of the normal hip hyaline cartilage is uniform.
25082625	3	142	theme	normal	417:422	arg1	hip					424:426	the normal hip	413:426	the normal hip	413:426	Knowledge of the T1ρ profile of hyaline cartilage in the normal hip is important for establishing a baseline against which comparisons can be made to experimental and clinical arthritic subjects.
25082625	6	143	theme	femoral	966:972	arg1	head					974:977	femoral head	966:977	femoral head	966:977	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	11	144	theme	zones	1914:1918	arg1	combination					1899:1909	a selective combination	1887:1909	a selective combination of zones and slices	1887:1929	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
25082625	15	145	theme	cartilage	2763:2771	arg1	health					2773:2778	cartilage health	2763:2778	cartilage health	2763:2778	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	15	146	theme	MRI	2853:2855	arg1	techniques					2857:2866	advanced, biochemical MRI techniques	2831:2866	advanced, biochemical MRI techniques for evaluating hyaline cartilage	2831:2899	This technique has the potential to facilitate basic science and clinical research by serving as a noninvasive surrogate or biomarker of cartilage health and thus may be added to the growing repertoire of advanced, biochemical MRI techniques for evaluating hyaline cartilage.
25082625	6	147	theme	acetabulum	951:960	arg1	bilayer					936:942	The weightbearing hyaline cartilage bilayer	900:942	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head	900:977	The weightbearing hyaline cartilage bilayer of the acetabulum and femoral head was evaluated on sagittal images and segmented into four zones: (1) anterior; (2) anterosuperior; (3) posterosuperior; and (4) and posterior.
25082625	13	148	from	concentrations	2396:2409	arg1	region					2437:2442	the more anterolateral region	2414:2442	the more anterolateral region of the normal hip hyaline cartilage	2414:2478	This study, through determination of lower T1ρ relaxation values, suggests inherently greater PG concentrations in the more anterolateral region of the normal hip hyaline cartilage.
25082625	11	149	theme	lower	1943:1947	arg1	values					1958:1963	lower mean T1ρ values	1943:1963	lower mean T1ρ values	1943:1963	With a selective combination of zones and slices, there were lower mean T1ρ values in the anterolateral-most region compared with the remainder of the weightbearing portion of the hip (28.6 msec versus 32.2 msec, respectively; p = 0.001).
24564751	6	0	theme	Salt	996:999	arg1	linkage					1001:1007	Salt linkage	996:1007	Salt linkage between lactic acid molecules and amino side chains of chitosan	996:1071	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	6	1	theme	acid	1120:1123	arg1	molecules					1125:1133	the lactic acid molecules	1109:1133	the lactic acid molecules	1109:1133	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	7	2	theme	other	1230:1234	arg1	water					1263:1267	water	1263:1267	water	1263:1267	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	7	2	theme	other	1230:1234	arg1	glycerol					1250:1257	glycerol	1250:1257	glycerol	1250:1257	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	7	2	theme	other	1230:1234	arg1	plasticizers					1236:1247	other plasticizers	1230:1247	other plasticizers (glycerol and water)	1230:1268	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	6	3	theme	lactic	1113:1118	arg1	molecules					1125:1133	the lactic acid molecules	1109:1133	the lactic acid molecules	1109:1133	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	8	4	theme	plasticized	1528:1538	arg1	chitosan					1540:1547	the plasticized chitosan	1524:1547	the plasticized chitosan	1524:1547	The thermal and thermomechanical properties of the plasticized chitosan were measured by thermogravimetric analysis, differential scanning calorimetric, and DMA.
24564751	9	5	theme	microstructure	1701:1714	arg1	levels					1691:1696	the different levels	1677:1696	the different levels of microstructure	1677:1714	These properties were correlated with the different levels of microstructure, including multiple structural units.
24564751	1	6	dep	scale	155:159	arg1	units					172:176	structural units	161:176	multiple length scale structural units	139:176	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	6	7	theme	acid	1024:1027	arg1	molecules					1029:1037	lactic acid molecules	1017:1037	lactic acid molecules	1017:1037	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	7	8	theme	thermal	1427:1433	arg1	energy					1435:1440	thermal energy	1427:1440	thermal energy	1427:1440	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	6	9	theme	lactic	1017:1022	arg1	molecules					1029:1037	lactic acid molecules	1017:1037	lactic acid molecules	1017:1037	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	9	10	theme	different	1681:1689	arg1	levels					1691:1696	the different levels	1677:1696	the different levels of microstructure	1677:1714	These properties were correlated with the different levels of microstructure, including multiple structural units.
24564751	1	11	theme	Plasticized	79:89	arg1	chitosan					91:98	Plasticized chitosan	79:98	Plasticized chitosan	79:98	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	6	12	theme	amino	1043:1047	arg1	chains					1054:1059	amino side chains	1043:1059	amino side chains of chitosan	1043:1071	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	2	13	theme	thermomechanical	348:363	arg1	mixing					365:370	thermomechanical mixing	348:370	thermomechanical mixing	348:370	Chitosan was successfully plasticized by thermomechanical mixing in the presence of concentrated lactic acid and glycerol using a batch mixer.
24564751	7	14	theme	mechanical	1406:1415	arg1	shear					1417:1421	mechanical shear	1406:1421	mechanical shear	1406:1421	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	4	15	theme	chitosan	658:665	arg1	microstructure					609:622	The microstructure	605:622	The microstructure of thermomechanically plasticized chitosan	605:665	The microstructure of thermomechanically plasticized chitosan was investigated by X-ray diffraction, scanning electron microscopy, and optical microscopy.
24564751	6	16	theme	phase	1204:1208	arg1	molecules					1175:1183	the chitosan molecules	1162:1183	the chitosan molecules of the crystalline phase	1162:1208	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	4	17	theme	scanning	706:713	arg1	microscopy					724:733	scanning electron microscopy	706:733	scanning electron microscopy	706:733	The microstructure of thermomechanically plasticized chitosan was investigated by X-ray diffraction, scanning electron microscopy, and optical microscopy.
24564751	2	18	theme	acid	411:414	arg1	presence					379:386	the presence	375:386	the presence of concentrated lactic acid and glycerol	375:427	Chitosan was successfully plasticized by thermomechanical mixing in the presence of concentrated lactic acid and glycerol using a batch mixer.
24564751	5	19	theme	additional	786:795	arg1	water					823:827	water	823:827	water	823:827	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	5	19	theme	additional	786:795	arg1	glycerol					811:818	glycerol	811:818	glycerol	811:818	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	5	19	theme	additional	786:795	arg1	plasticizers					797:808	additional plasticizers	786:808	additional plasticizers (glycerol or water)	786:828	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	7	20	theme	first	1465:1469	arg1	time					1471:1474	the first time	1461:1474	the first time	1461:1474	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	9	21	theme	multiple	1727:1734	arg1	units					1747:1751	multiple structural units	1727:1751	multiple structural units	1727:1751	These properties were correlated with the different levels of microstructure, including multiple structural units.
24564751	6	22	theme	crystalline	1192:1202	arg1	phase					1204:1208	the crystalline phase	1188:1208	the crystalline phase	1188:1208	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	2	23	theme	lactic	404:409	arg1	acid					411:414	concentrated lactic acid	391:414	concentrated lactic acid	391:414	Chitosan was successfully plasticized by thermomechanical mixing in the presence of concentrated lactic acid and glycerol using a batch mixer.
24564751	8	24	theme	differential	1594:1605	arg1	calorimetric					1616:1627	differential scanning calorimetric	1594:1627	differential scanning calorimetric	1594:1627	The thermal and thermomechanical properties of the plasticized chitosan were measured by thermogravimetric analysis, differential scanning calorimetric, and DMA.
24564751	9	25	theme	structural	1736:1745	arg1	units					1747:1751	multiple structural units	1727:1751	multiple structural units	1727:1751	These properties were correlated with the different levels of microstructure, including multiple structural units.
24564751	4	26	theme	X-ray	687:691	arg1	diffraction					693:703	X-ray diffraction	687:703	X-ray diffraction	687:703	The microstructure of thermomechanically plasticized chitosan was investigated by X-ray diffraction, scanning electron microscopy, and optical microscopy.
24564751	1	27	theme	hierarchical	105:116	arg1	structure					118:126	hierarchical structure	105:126	hierarchical structure	105:126	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	2	28	theme	concentrated	391:402	arg1	acid					411:414	concentrated lactic acid	391:414	concentrated lactic acid	391:414	Chitosan was successfully plasticized by thermomechanical mixing in the presence of concentrated lactic acid and glycerol using a batch mixer.
24564751	0	29	theme	Hierarchical	0:11	arg1	structure					13:21	Hierarchical structure	0:21	Hierarchical structure	0:21	Hierarchical structure and physicochemical properties of plasticized chitosan.
24564751	1	30	theme	melt	198:201	arg1	method					210:215	a "melt"-based method	195:215	a "melt"-based method	195:215	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	1	30	theme	melt	198:201	arg1	mixing					244:249	thermomechanical mixing	227:249	thermomechanical mixing	227:249	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	0	31	theme	physicochemical	27:41	arg1	properties					43:52	physicochemical properties	27:52	physicochemical properties	27:52	Hierarchical structure and physicochemical properties of plasticized chitosan.
24564751	7	32	dep	plasticizers	1236:1247	arg1	the					1214:1216	the	1214:1216	the	1214:1216	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	7	32	dep	plasticizers	1236:1247	arg1	water					1263:1267	water	1263:1267	water	1263:1267	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	7	32	dep	plasticizers	1236:1247	arg1	glycerol					1250:1257	glycerol	1250:1257	glycerol	1250:1257	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	7	32	dep	plasticizers	1236:1247	arg1	presence					1218:1225	presence	1218:1225	presence	1218:1225	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	7	32	dep	plasticizers	1236:1247	arg1	plasticizers					1236:1247	other plasticizers	1230:1247	other plasticizers (glycerol and water)	1230:1268	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	3	33	theme	protonation	563:573	arg1	agent					575:579	protonation agent	563:579	protonation agent as well as plasticizer	563:602	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	3	33	theme	protonation	563:573	arg1	acid					546:549	concentrated lactic acid	526:549	concentrated lactic acid	526:549	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	5	34	theme	chitosan	868:875	arg1	crystallinity					835:847	the crystallinity	831:847	the crystallinity of the plasticized chitosan	831:875	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	2	35	theme	batch	437:441	arg1	mixer					443:447	a batch mixer	435:447	a batch mixer	435:447	Chitosan was successfully plasticized by thermomechanical mixing in the presence of concentrated lactic acid and glycerol using a batch mixer.
24564751	1	36	theme	usual	270:274	arg1	procedure					296:304	the usual casting-evaporation procedure	266:304	the usual casting-evaporation procedure	266:304	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	6	37	theme	chitosan	1166:1173	arg1	molecules					1175:1183	the chitosan molecules	1162:1183	the chitosan molecules of the crystalline phase	1162:1208	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	8	38	theme	scanning	1607:1614	arg1	calorimetric					1616:1627	differential scanning calorimetric	1594:1627	differential scanning calorimetric	1594:1627	The thermal and thermomechanical properties of the plasticized chitosan were measured by thermogravimetric analysis, differential scanning calorimetric, and DMA.
24564751	1	39	theme	-based	203:208	arg1	method					210:215	a "melt"-based method	195:215	a "melt"-based method	195:215	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	1	39	theme	-based	203:208	arg1	mixing					244:249	thermomechanical mixing	227:249	thermomechanical mixing	227:249	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	1	40	with	chitosan	91:98	arg1	structure					118:126	hierarchical structure	105:126	hierarchical structure	105:126	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	1	41	theme	casting-evaporation	276:294	arg1	procedure					296:304	the usual casting-evaporation procedure	266:304	the usual casting-evaporation procedure	266:304	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	5	42	dep	plasticizers	797:808	arg1	water					823:827	water	823:827	water	823:827	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	5	42	dep	plasticizers	797:808	arg1	glycerol					811:818	glycerol	811:818	glycerol	811:818	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	5	42	dep	plasticizers	797:808	arg1	plasticizers					797:808	additional plasticizers	786:808	additional plasticizers (glycerol or water)	786:828	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	3	43	theme	plasticization	460:473	arg1	formulations					475:486	Different plasticization formulations	450:486	Different plasticization formulations	450:486	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	3	44	used	used	555:558	arg2	acid					546:549	concentrated lactic acid	526:549	concentrated lactic acid	526:549	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	3	44	used	used	555:558	arg2	agent					575:579	protonation agent	563:579	protonation agent as well as plasticizer	563:602	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	3	44	used	used	555:558	arg2	plasticizer					592:602	plasticizer	592:602	protonation agent as well as plasticizer	563:602	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	2	45	theme	glycerol	420:427	arg1	presence					379:386	the presence	375:386	the presence of concentrated lactic acid and glycerol	375:427	Chitosan was successfully plasticized by thermomechanical mixing in the presence of concentrated lactic acid and glycerol using a batch mixer.
24564751	4	46	theme	optical	740:746	arg1	microscopy					748:757	optical microscopy	740:757	optical microscopy	740:757	The microstructure of thermomechanically plasticized chitosan was investigated by X-ray diffraction, scanning electron microscopy, and optical microscopy.
24564751	6	47	theme	side	1049:1052	arg1	chains					1054:1059	amino side chains	1043:1059	amino side chains of chitosan	1043:1071	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	6	48	theme	chitosan	1064:1071	arg1	molecules					1029:1037	lactic acid molecules	1017:1037	lactic acid molecules	1017:1037	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	6	48	theme	chitosan	1064:1071	arg1	chains					1054:1059	amino side chains	1043:1059	amino side chains of chitosan	1043:1071	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	0	49	theme	chitosan	69:76	arg1	structure					13:21	Hierarchical structure	0:21	Hierarchical structure	0:21	Hierarchical structure and physicochemical properties of plasticized chitosan.
24564751	0	49	theme	chitosan	69:76	arg1	properties					43:52	physicochemical properties	27:52	physicochemical properties	27:52	Hierarchical structure and physicochemical properties of plasticized chitosan.
24564751	5	50	theme	chitosan	915:922	arg1	powder					924:929	the original chitosan powder	902:929	the original chitosan powder	902:929	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	7	51	theme	structural	1289:1298	arg1	nanofibrils					1336:1346	nanofibrils	1336:1346	nanofibrils	1336:1346	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	7	51	theme	structural	1289:1298	arg1	clusters					1360:1367	nanofibril clusters	1349:1367	nanofibril clusters	1349:1367	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	7	51	theme	structural	1289:1298	arg1	phase					1329:1333	an amorphous phase	1316:1333	an amorphous phase	1316:1333	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	7	51	theme	structural	1289:1298	arg1	microfibers					1374:1384	microfibers	1374:1384	microfibers	1374:1384	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	7	51	theme	structural	1289:1298	arg1	units					1300:1304	structural units	1289:1304	structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers	1289:1384	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	8	52	theme	thermogravimetric	1566:1582	arg1	analysis					1584:1591	thermogravimetric analysis	1566:1591	thermogravimetric analysis	1566:1591	The thermal and thermomechanical properties of the plasticized chitosan were measured by thermogravimetric analysis, differential scanning calorimetric, and DMA.
24564751	1	53	theme	multiple	139:146	arg1	scale					155:159	multiple length scale	139:159	multiple length scale structural units	139:176	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	0	54	theme	plasticized	57:67	arg1	chitosan					69:76	plasticized chitosan	57:76	plasticized chitosan	57:76	Hierarchical structure and physicochemical properties of plasticized chitosan.
24564751	8	55	theme	thermal	1481:1487	arg1	properties					1510:1519	The thermal and thermomechanical properties	1477:1519	The thermal and thermomechanical properties of the plasticized chitosan	1477:1547	The thermal and thermomechanical properties of the plasticized chitosan were measured by thermogravimetric analysis, differential scanning calorimetric, and DMA.
24564751	7	56	theme	nanofibril	1349:1358	arg1	clusters					1360:1367	nanofibril clusters	1349:1367	nanofibril clusters	1349:1367	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	1	57	theme	length	148:153	arg1	scale					155:159	multiple length scale	139:159	multiple length scale structural units	139:176	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	7	58	theme	various	1271:1277	arg1	levels					1279:1284	various levels	1271:1284	various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers	1271:1384	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	5	59	theme	original	906:913	arg1	powder					924:929	the original chitosan powder	902:929	the original chitosan powder	902:929	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	4	60	theme	electron	715:722	arg1	microscopy					724:733	scanning electron microscopy	706:733	scanning electron microscopy	706:733	The microstructure of thermomechanically plasticized chitosan was investigated by X-ray diffraction, scanning electron microscopy, and optical microscopy.
24564751	5	61	theme	additional	978:987	arg1	water					989:993	additional water	978:993	additional water	978:993	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	7	62	theme	units	1300:1304	arg1	levels					1279:1284	various levels	1271:1284	various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers	1271:1384	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	6	63	theme	FTIR	1090:1093	arg1	spectroscopy					1095:1106	FTIR spectroscopy	1090:1106	FTIR spectroscopy	1090:1106	Salt linkage between lactic acid molecules and amino side chains of chitosan was confirmed by FTIR spectroscopy: the lactic acid molecules expanded the space between the chitosan molecules of the crystalline phase.
24564751	1	64	theme	structural	161:170	arg1	units					172:176	structural units	161:176	multiple length scale structural units	139:176	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	5	65	theme	plasticizers	797:808	arg1	water					823:827	water	823:827	water	823:827	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	5	65	theme	plasticizers	797:808	arg1	glycerol					811:818	glycerol	811:818	glycerol	811:818	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	5	65	theme	plasticizers	797:808	arg1	amount					776:781	amount	776:781	amount of additional plasticizers (glycerol or water)	776:828	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	5	65	theme	plasticizers	797:808	arg1	plasticizers					797:808	additional plasticizers	786:808	additional plasticizers (glycerol or water)	786:828	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	8	66	theme	thermomechanical	1493:1508	arg1	properties					1510:1519	The thermal and thermomechanical properties	1477:1519	The thermal and thermomechanical properties of the plasticized chitosan	1477:1547	The thermal and thermomechanical properties of the plasticized chitosan were measured by thermogravimetric analysis, differential scanning calorimetric, and DMA.
24564751	7	67	theme	amorphous	1319:1327	arg1	phase					1329:1333	an amorphous phase	1316:1333	an amorphous phase	1316:1333	In the presence of other plasticizers (glycerol and water), various levels of structural units including an amorphous phase, nanofibrils, nanofibril clusters, and microfibers were produced under mechanical shear and thermal energy and identified for the first time.
24564751	3	68	theme	concentrated	526:537	arg1	agent					575:579	protonation agent	563:579	protonation agent as well as plasticizer	563:602	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	3	68	theme	concentrated	526:537	arg1	acid					546:549	concentrated lactic acid	526:549	concentrated lactic acid	526:549	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	3	68	theme	concentrated	526:537	arg1	plasticizer					592:602	plasticizer	592:602	protonation agent as well as plasticizer	563:602	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	3	69	theme	Different	450:458	arg1	formulations					475:486	Different plasticization formulations	450:486	Different plasticization formulations	450:486	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	4	70	theme	plasticized	646:656	arg1	chitosan					658:665	thermomechanically plasticized chitosan	627:665	thermomechanically plasticized chitosan	627:665	The microstructure of thermomechanically plasticized chitosan was investigated by X-ray diffraction, scanning electron microscopy, and optical microscopy.
24564751	5	71	theme	plasticized	856:866	arg1	chitosan					868:875	the plasticized chitosan	852:875	the plasticized chitosan	852:875	With increasing amount of additional plasticizers (glycerol or water), the crystallinity of the plasticized chitosan decreased from 63.7% for the original chitosan powder to almost zero for the sample plasticized with additional water.
24564751	3	72	theme	lactic	539:544	arg1	agent					575:579	protonation agent	563:579	protonation agent as well as plasticizer	563:602	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	3	72	theme	lactic	539:544	arg1	acid					546:549	concentrated lactic acid	526:549	concentrated lactic acid	526:549	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	3	72	theme	lactic	539:544	arg1	plasticizer					592:602	plasticizer	592:602	protonation agent as well as plasticizer	563:602	Different plasticization formulations were compared in this study, in which concentrated lactic acid was used as protonation agent as well as plasticizer.
24564751	8	73	theme	chitosan	1540:1547	arg1	properties					1510:1519	The thermal and thermomechanical properties	1477:1519	The thermal and thermomechanical properties of the plasticized chitosan	1477:1547	The thermal and thermomechanical properties of the plasticized chitosan were measured by thermogravimetric analysis, differential scanning calorimetric, and DMA.
24564751	1	74	theme	thermomechanical	227:242	arg1	method					210:215	a "melt"-based method	195:215	a "melt"-based method	195:215	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
24564751	1	74	theme	thermomechanical	227:242	arg1	mixing					244:249	thermomechanical mixing	227:249	thermomechanical mixing	227:249	Plasticized chitosan with hierarchical structure, including multiple length scale structural units, was prepared by a "melt"-based method, that is, thermomechanical mixing, as opposed to the usual casting-evaporation procedure.
26689482	2	0	theme	GC	328:329	arg1	content					331:337	a GC content	326:337	a GC content of 45.9%	326:346	Here, we present the complete genome sequence, which consists of one circular chromosome of 4,416,600 bp with a GC content of 45.9%.
26689482	0	1	theme	gilvus	40:45	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Agarivorans gilvus WH0801(T)	0:55	Complete genome sequence of Agarivorans gilvus WH0801(T), an agarase-producing bacterium isolated from seaweed.
26689482	0	1	theme	gilvus	40:45	arg1	bacterium					79:87	an agarase-producing bacterium	58:87	an agarase-producing bacterium isolated from seaweed	58:109	Complete genome sequence of Agarivorans gilvus WH0801(T), an agarase-producing bacterium isolated from seaweed.
26689482	2	2	theme	circular	285:292	arg1	chromosome					294:303	one circular chromosome	281:303	one circular chromosome of 4,416,600 bp with a GC content of 45.9%	281:346	Here, we present the complete genome sequence, which consists of one circular chromosome of 4,416,600 bp with a GC content of 45.9%.
26689482	1	3	theme	Agarivorans	112:122	arg1	bacterium					163:171	an agarase-producing bacterium	142:171	an agarase-producing bacterium	142:171	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	1	3	theme	Agarivorans	112:122	arg1	T					138:138	T	138:138	T	138:138	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	1	3	theme	Agarivorans	112:122	arg1	WH0801					131:136	Agarivorans gilvus WH0801	112:136	Agarivorans gilvus WH0801(T)	112:139	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	0	4	theme	genome	9:14	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Agarivorans gilvus WH0801(T)	0:55	Complete genome sequence of Agarivorans gilvus WH0801(T), an agarase-producing bacterium isolated from seaweed.
26689482	0	4	theme	genome	9:14	arg1	bacterium					79:87	an agarase-producing bacterium	58:87	an agarase-producing bacterium isolated from seaweed	58:109	Complete genome sequence of Agarivorans gilvus WH0801(T), an agarase-producing bacterium isolated from seaweed.
26689482	3	5	theme	genetic	354:360	arg1	information					362:372	This genetic information	349:372	This genetic information	349:372	This genetic information will provide insight into biotechnological applications of producing agar for food and industry.
26689482	2	6	theme	%	346:346	arg1	content					331:337	a GC content	326:337	a GC content of 45.9%	326:346	Here, we present the complete genome sequence, which consists of one circular chromosome of 4,416,600 bp with a GC content of 45.9%.
26689482	1	7	theme	gilvus	124:129	arg1	bacterium					163:171	an agarase-producing bacterium	142:171	an agarase-producing bacterium	142:171	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	1	7	theme	gilvus	124:129	arg1	T					138:138	T	138:138	T	138:138	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	1	7	theme	gilvus	124:129	arg1	WH0801					131:136	Agarivorans gilvus WH0801	112:136	Agarivorans gilvus WH0801(T)	112:139	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	1	8	theme	agarase-producing	145:161	arg1	bacterium					163:171	an agarase-producing bacterium	142:171	an agarase-producing bacterium	142:171	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	1	8	theme	agarase-producing	145:161	arg1	WH0801					131:136	Agarivorans gilvus WH0801	112:136	Agarivorans gilvus WH0801(T)	112:139	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	0	9	theme	Complete	0:7	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Agarivorans gilvus WH0801(T)	0:55	Complete genome sequence of Agarivorans gilvus WH0801(T), an agarase-producing bacterium isolated from seaweed.
26689482	0	9	theme	Complete	0:7	arg1	bacterium					79:87	an agarase-producing bacterium	58:87	an agarase-producing bacterium isolated from seaweed	58:109	Complete genome sequence of Agarivorans gilvus WH0801(T), an agarase-producing bacterium isolated from seaweed.
26689482	2	10	theme	genome	246:251	arg1	sequence					253:260	the complete genome sequence	233:260	the complete genome sequence	233:260	Here, we present the complete genome sequence, which consists of one circular chromosome of 4,416,600 bp with a GC content of 45.9%.
26689482	1	11	attach	isolated	178:185	arg2	T					138:138	T	138:138	T	138:138	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	1	11	attach	isolated	178:185	arg2	bacterium					163:171	an agarase-producing bacterium	142:171	an agarase-producing bacterium	142:171	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	1	11	attach	isolated	178:185	arg2	WH0801					131:136	Agarivorans gilvus WH0801	112:136	Agarivorans gilvus WH0801(T)	112:139	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	1	11	attach	isolated	178:185	arg1	surface					196:202	the surface	192:202	the surface of seaweed	192:213	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
26689482	0	12	theme	agarase-producing	61:77	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Agarivorans gilvus WH0801(T)	0:55	Complete genome sequence of Agarivorans gilvus WH0801(T), an agarase-producing bacterium isolated from seaweed.
26689482	0	12	theme	agarase-producing	61:77	arg1	bacterium					79:87	an agarase-producing bacterium	58:87	an agarase-producing bacterium isolated from seaweed	58:109	Complete genome sequence of Agarivorans gilvus WH0801(T), an agarase-producing bacterium isolated from seaweed.
26689482	2	13	theme	complete	237:244	arg1	sequence					253:260	the complete genome sequence	233:260	the complete genome sequence	233:260	Here, we present the complete genome sequence, which consists of one circular chromosome of 4,416,600 bp with a GC content of 45.9%.
26689482	2	14	with	bp	318:319	arg1	content					331:337	a GC content	326:337	a GC content of 45.9%	326:346	Here, we present the complete genome sequence, which consists of one circular chromosome of 4,416,600 bp with a GC content of 45.9%.
26689482	0	15	attach	isolated	89:96	arg2	bacterium					79:87	an agarase-producing bacterium	58:87	an agarase-producing bacterium isolated from seaweed	58:109	Complete genome sequence of Agarivorans gilvus WH0801(T), an agarase-producing bacterium isolated from seaweed.
26689482	0	15	attach	isolated	89:96	arg1	seaweed					103:109	seaweed	103:109	seaweed	103:109	Complete genome sequence of Agarivorans gilvus WH0801(T), an agarase-producing bacterium isolated from seaweed.
26689482	0	15	attach	isolated	89:96	arg2	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Agarivorans gilvus WH0801(T)	0:55	Complete genome sequence of Agarivorans gilvus WH0801(T), an agarase-producing bacterium isolated from seaweed.
26689482	2	16	theme	bp	318:319	arg1	chromosome					294:303	one circular chromosome	281:303	one circular chromosome of 4,416,600 bp with a GC content of 45.9%	281:346	Here, we present the complete genome sequence, which consists of one circular chromosome of 4,416,600 bp with a GC content of 45.9%.
26689482	3	17	theme	biotechnological	400:415	arg1	applications					417:428	biotechnological applications	400:428	biotechnological applications of producing agar for food and industry	400:468	This genetic information will provide insight into biotechnological applications of producing agar for food and industry.
26689482	1	18	theme	seaweed	207:213	arg1	surface					196:202	the surface	192:202	the surface of seaweed	192:213	Agarivorans gilvus WH0801(T), an agarase-producing bacterium, was isolated from the surface of seaweed.
27021080	4	0	from	use	668:670	arg1	treatment					679:687	the treatment	675:687	the treatment of wounds with profuse exudation	675:720	High absorption capacity recommends its use in the treatment of wounds with profuse exudation.
27021080	4	1	with	treatment	679:687	arg1	exudation					712:720	profuse exudation	704:720	profuse exudation	704:720	High absorption capacity recommends its use in the treatment of wounds with profuse exudation.
27021080	1	2	theme	natural	135:141	arg1	chitosan					154:161	natural biopolymer chitosan	135:161	natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol)	135:238	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	0	3	theme	Natural	78:84	arg1	Chitosan					68:75	Chitosan	68:75	Chitosan (Natural Biopolymer)	68:96	Soft Tissue Regeneration under the Effect of Wound Coating Based on Chitosan (Natural Biopolymer).
27021080	0	3	theme	Natural	78:84	arg1	Biopolymer					86:95	Natural Biopolymer	78:95	Natural Biopolymer	78:95	Soft Tissue Regeneration under the Effect of Wound Coating Based on Chitosan (Natural Biopolymer).
27021080	2	4	theme	male	263:266	arg1	rats					268:271	albino male rats	256:271	albino male rats	256:271	Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin.
27021080	2	5	theme	antioxidant	304:314	arg1	effects					335:341	its regeneratory, antioxidant, and antibacterial effects	286:341	its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin	286:384	Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin.
27021080	2	6	theme	albino	256:261	arg1	rats					268:271	albino male rats	256:271	albino male rats	256:271	Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin.
27021080	1	7	theme	biopolymer	143:152	arg1	chitosan					154:161	natural biopolymer chitosan	135:161	natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol)	135:238	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	3	8	theme	buffer	419:424	arg1	component					426:434	buffer component	419:434	buffer component	419:434	Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites.
27021080	0	9	theme	Tissue	5:10	arg1	Regeneration					12:23	Soft Tissue Regeneration	0:23	Soft Tissue Regeneration under the Effect of Wound Coating	0:57	Soft Tissue Regeneration under the Effect of Wound Coating Based on Chitosan (Natural Biopolymer).
27021080	2	10	from	effects	335:341	arg1	wounds					346:351	wounds	346:351	wounds involving all layers of the skin	346:384	Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin.
27021080	4	11	theme	profuse	704:710	arg1	exudation					712:720	profuse exudation	704:720	profuse exudation	704:720	High absorption capacity recommends its use in the treatment of wounds with profuse exudation.
27021080	3	12	theme	chemical	394:401	arg1	composition					403:413	chemical composition	394:413	chemical composition	394:413	Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites.
27021080	0	13	theme	Soft	0:3	arg1	Regeneration					12:23	Soft Tissue Regeneration	0:23	Soft Tissue Regeneration under the Effect of Wound Coating	0:57	Soft Tissue Regeneration under the Effect of Wound Coating Based on Chitosan (Natural Biopolymer).
27021080	2	14	theme	regeneratory	290:301	arg1	effects					335:341	its regeneratory, antioxidant, and antibacterial effects	286:341	its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin	286:384	Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin.
27021080	1	15	theme	additional	168:177	arg1	ceruloplasmin					191:203	ceruloplasmin	191:203	ceruloplasmin	191:203	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	1	15	theme	additional	168:177	arg1	acid					220:223	L-asparaginic acid	206:223	L-asparaginic acid	206:223	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	1	15	theme	additional	168:177	arg1	components					179:188	additional components	168:188	additional components (ceruloplasmin, L-asparaginic acid, and glycerol)	168:238	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	1	15	theme	additional	168:177	arg1	glycerol					230:237	glycerol	230:237	glycerol	230:237	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	3	16	theme	reparative	536:545	arg1	regeneration					547:558	the reparative regeneration	532:558	the reparative regeneration	532:558	Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites.
27021080	3	17	theme	process	501:507	arg1	phases					481:486	all phases	477:486	all phases of the wound process	477:507	Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites.
27021080	3	18	theme	injured	613:619	arg1	sites					621:625	injured sites	613:625	injured sites	613:625	Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites.
27021080	2	19	from	Experiments	241:251	arg1	rats					268:271	albino male rats	256:271	albino male rats	256:271	Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin.
27021080	3	20	theme	biodegraded	441:451	arg1	coating					459:465	the biodegraded wound coating	437:465	the biodegraded wound coating	437:465	Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites.
27021080	3	21	theme	sites	621:625	arg1	restoration					598:608	anatomic and functional restoration	574:608	anatomic and functional restoration of injured sites	574:625	Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites.
27021080	4	22	theme	absorption	633:642	arg1	capacity					644:651	High absorption capacity	628:651	High absorption capacity	628:651	High absorption capacity recommends its use in the treatment of wounds with profuse exudation.
27021080	4	23	theme	High	628:631	arg1	capacity					644:651	High absorption capacity	628:651	High absorption capacity	628:651	High absorption capacity recommends its use in the treatment of wounds with profuse exudation.
27021080	2	24	theme	skin	381:384	arg1	layers					367:372	all layers	363:372	all layers of the skin	363:384	Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin.
27021080	0	25	theme	Wound	45:49	arg1	Coating					51:57	Wound Coating	45:57	Wound Coating	45:57	Soft Tissue Regeneration under the Effect of Wound Coating Based on Chitosan (Natural Biopolymer).
27021080	2	26	theme	antibacterial	321:333	arg1	effects					335:341	its regeneratory, antioxidant, and antibacterial effects	286:341	its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin	286:384	Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin.
27021080	3	27	theme	wound	453:457	arg1	coating					459:465	the biodegraded wound coating	437:465	the biodegraded wound coating	437:465	Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites.
27021080	1	28	theme	L-asparaginic	206:218	arg1	acid					220:223	L-asparaginic acid	206:223	L-asparaginic acid	206:223	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	1	28	theme	L-asparaginic	206:218	arg1	components					179:188	additional components	168:188	additional components (ceruloplasmin, L-asparaginic acid, and glycerol)	168:238	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	1	29	with	chitosan	154:161	arg1	ceruloplasmin					191:203	ceruloplasmin	191:203	ceruloplasmin	191:203	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	1	29	with	chitosan	154:161	arg1	acid					220:223	L-asparaginic acid	206:223	L-asparaginic acid	206:223	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	1	29	with	chitosan	154:161	arg1	components					179:188	additional components	168:188	additional components (ceruloplasmin, L-asparaginic acid, and glycerol)	168:238	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	1	29	with	chitosan	154:161	arg1	glycerol					230:237	glycerol	230:237	glycerol	230:237	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	3	30	theme	functional	587:596	arg1	restoration					598:608	anatomic and functional restoration	574:608	anatomic and functional restoration of injured sites	574:625	Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites.
27021080	1	31	theme	wound	112:116	arg1	coating					118:124	wound coating	112:124	wound coating	112:124	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	4	32	theme	wounds	692:697	arg1	treatment					679:687	the treatment	675:687	the treatment of wounds with profuse exudation	675:720	High absorption capacity recommends its use in the treatment of wounds with profuse exudation.
27021080	1	33	dep	components	179:188	arg1	components					179:188	additional components	168:188	additional components (ceruloplasmin, L-asparaginic acid, and glycerol)	168:238	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	1	33	dep	components	179:188	arg1	ceruloplasmin					191:203	ceruloplasmin	191:203	ceruloplasmin	191:203	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	1	33	dep	components	179:188	arg1	acid					220:223	L-asparaginic acid	206:223	L-asparaginic acid	206:223	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	1	33	dep	components	179:188	arg1	glycerol					230:237	glycerol	230:237	glycerol	230:237	We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol).
27021080	0	34	theme	Coating	51:57	arg1	Effect					35:40	the Effect	31:40	the Effect of Wound Coating	31:57	Soft Tissue Regeneration under the Effect of Wound Coating Based on Chitosan (Natural Biopolymer).
27021080	3	35	theme	anatomic	574:581	arg1	restoration					598:608	anatomic and functional restoration	574:608	anatomic and functional restoration of injured sites	574:625	Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites.
27021080	3	36	theme	wound	495:499	arg1	process					501:507	the wound process	491:507	the wound process	491:507	Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites.
25661466	9	0	theme	Transcript	1357:1366	arg1	profiling					1368:1376	Transcript profiling	1357:1376	Transcript profiling of these genes across sorghum tissues	1357:1414	Transcript profiling of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, CslF6 transcripts dominated.
25661466	6	1	theme	electron	1050:1057	arg1	microscopy					1059:1068	fluorescence and transmission electron microscopy	1020:1068	microscopy	1059:1068	The distribution of (1,3;1,4)-β-glucan was examined using a specific antibody and observed with fluorescence and transmission electron microscopy.
25661466	1	2	theme	human	210:214	arg1	health					216:221	human health	210:221	human health	210:221	In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications.
25661466	0	3	dep	1,4	63:65	arg1	of					55:56	of	55:56	of	55:56	Distribution, structure and biosynthetic gene families of (1,3;1,4)-β-glucan in Sorghum bicolor.
25661466	3	4	theme	sorghum	446:452	arg1	tissues					454:460	sorghum tissues	446:460	sorghum tissues	446:460	Here, the amount of (1,3;1,4)-β-glucan present in sorghum tissues was measured using a Megazyme assay.
25661466	9	5	theme	genes	1387:1391	arg1	profiling					1368:1376	Transcript profiling	1357:1376	Transcript profiling of these genes across sorghum tissues	1357:1414	Transcript profiling of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, CslF6 transcripts dominated.
25661466	3	6	theme	present	435:441	arg1	amount					406:411	the amount	402:411	the amount of (1,3;1,4)-β-glucan present in sorghum tissues	402:460	Here, the amount of (1,3;1,4)-β-glucan present in sorghum tissues was measured using a Megazyme assay.
25661466	3	6	theme	present	435:441	arg1	present					435:441	(1,3;1,4)-β-glucan present	416:441	(1,3;1,4)-β-glucan present	416:441	Here, the amount of (1,3;1,4)-β-glucan present in sorghum tissues was measured using a Megazyme assay.
25661466	1	7	theme	polysaccharides	133:147	arg1	presence					113:120	the presence	109:120	the presence of soluble polysaccharides including (1,3;1,4)-β-glucan	109:176	In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications.
25661466	9	8	theme	sorghum	1400:1406	arg1	tissues					1408:1414	sorghum tissues	1400:1414	sorghum tissues	1400:1414	Transcript profiling of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, CslF6 transcripts dominated.
25661466	8	9	dep	1,3	1243:1245	arg1	1,4					1247:1249	1,4	1247:1249	1,3;1,4	1243:1249	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	2	10	theme	Sorghum	262:268	arg1	L.					279:280	L.	279:280	L.	279:280	Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides.
25661466	2	10	theme	Sorghum	262:268	arg1	bicolor					270:276	Sorghum bicolor	262:276	Sorghum bicolor (L.) Moench	262:288	Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides.
25661466	4	11	from	grain	536:540	arg1	present					521:527	present	521:527	present	521:527	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	6	12	dep	1,3	945:947	arg1	1,4					949:951	1,4	949:951	1,4	949:951	The distribution of (1,3;1,4)-β-glucan was examined using a specific antibody and observed with fluorescence and transmission electron microscopy.
25661466	8	13	theme	genes	1205:1209	arg1	orthologs					1192:1200	The sorghum orthologs	1180:1200	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families	1180:1341	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	9	14	theme	real-time	1438:1446	arg1	reaction					1478:1485	real-time quantitative polymerase chain reaction	1438:1485	real-time quantitative polymerase chain reaction	1438:1485	Transcript profiling of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, CslF6 transcripts dominated.
25661466	7	15	theme	-β-glucan	1126:1134	arg1	distribution					1101:1112	a variable distribution	1090:1112	a variable distribution of (1,3;1,4)-β-glucan	1090:1134	Micrographs showed a variable distribution of (1,3;1,4)-β-glucan influenced by temporal and spatial factors.
25661466	6	16	dep	-β-glucan	953:961	arg1	1,3					945:947	1,3	945:947	1,3;1,4	945:951	The distribution of (1,3;1,4)-β-glucan was examined using a specific antibody and observed with fluorescence and transmission electron microscopy.
25661466	3	17	theme	Megazyme	483:490	arg1	assay					492:496	a Megazyme assay	481:496	a Megazyme assay	481:496	Here, the amount of (1,3;1,4)-β-glucan present in sorghum tissues was measured using a Megazyme assay.
25661466	3	18	dep	present	435:441	arg1	1,3					417:419	1,3	417:419	1,3;1,4	417:423	Here, the amount of (1,3;1,4)-β-glucan present in sorghum tissues was measured using a Megazyme assay.
25661466	7	19	dep	-β-glucan	1126:1134	arg1	1,3					1118:1120	1,3	1118:1120	1,3;1,4	1118:1124	Micrographs showed a variable distribution of (1,3;1,4)-β-glucan influenced by temporal and spatial factors.
25661466	9	20	theme	polymerase	1461:1470	arg1	reaction					1478:1485	real-time quantitative polymerase chain reaction	1438:1485	real-time quantitative polymerase chain reaction	1438:1485	Transcript profiling of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, CslF6 transcripts dominated.
25661466	5	21	dep	-β-glucan	684:692	arg1	1,3					676:678	1,3	676:678	1,3;1,4	676:682	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	6	22	theme	-β-glucan	953:961	arg1	distribution					928:939	The distribution	924:939	The distribution of (1,3;1,4)-β-glucan	924:961	The distribution of (1,3;1,4)-β-glucan was examined using a specific antibody and observed with fluorescence and transmission electron microscopy.
25661466	5	23	theme	vegetative	880:889	arg1	tissues					891:897	vegetative tissues	880:897	vegetative tissues	880:897	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	2	24	theme	soluble	371:377	arg1	polysaccharides					379:393	such soluble polysaccharides	366:393	such soluble polysaccharides	366:393	Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides.
25661466	0	25	theme	1,3	59:61	arg1	Distribution					0:11	Distribution	0:11	Distribution	0:11	Distribution, structure and biosynthetic gene families of (1,3;1,4)-β-glucan in Sorghum bicolor.
25661466	0	25	theme	1,3	59:61	arg1	1,4					63:65	1,3;1,4	59:65	1,3;1,4	59:65	Distribution, structure and biosynthetic gene families of (1,3;1,4)-β-glucan in Sorghum bicolor.
25661466	1	26	theme	animal	224:229	arg1	feed					231:234	animal feed	224:234	animal feed	224:234	In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications.
25661466	9	27	theme	CslF6	1526:1530	arg1	transcripts					1532:1542	CslF6 transcripts	1526:1542	CslF6 transcripts	1526:1542	Transcript profiling of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, CslF6 transcripts dominated.
25661466	4	28	theme	greater	593:599	arg1	quantity					601:608	a greater quantity	591:608	a greater quantity in vegetative tissues	591:630	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	5	29	theme	residues	754:761	arg1	ratio					713:717	the ratio	709:717	the ratio of cellotriosyl and cellotetraosyl residues	709:761	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	0	30	theme	biosynthetic	28:39	arg1	families					46:53	biosynthetic gene families	28:53	biosynthetic gene families	28:53	Distribution, structure and biosynthetic gene families of (1,3;1,4)-β-glucan in Sorghum bicolor.
25661466	3	31	dep	1,3	417:419	arg1	1,4					421:423	1,4	421:423	1,3;1,4	417:423	Here, the amount of (1,3;1,4)-β-glucan present in sorghum tissues was measured using a Megazyme assay.
25661466	8	32	theme	synthase-like	1301:1313	arg1	F					1321:1321	the Cellulose synthase-like (Csl) F	1287:1321	the Cellulose synthase-like (Csl) F	1287:1321	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	5	33	dep	1,3	676:678	arg1	1,4					680:682	1,4	680:682	1,3;1,4	676:682	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	8	34	theme	Csl	1316:1318	arg1	F					1321:1321	the Cellulose synthase-like (Csl) F	1287:1321	the Cellulose synthase-like (Csl) F	1287:1321	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	8	35	theme	sorghum	1184:1190	arg1	orthologs					1192:1200	The sorghum orthologs	1180:1200	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families	1180:1341	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	5	36	theme	performance	785:795	arg1	HPLC					820:823	HPLC	820:823	HPLC	820:823	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	5	36	theme	performance	785:795	arg1	chromatography					804:817	high performance liquid chromatography	780:817	high performance liquid chromatography (HPLC)	780:824	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	8	37	theme	F	1321:1321	arg1	families					1334:1341	the Cellulose synthase-like (Csl) F and H gene families	1287:1341	the Cellulose synthase-like (Csl) F and H gene families	1287:1341	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	1	38	dep	1,3	160:162	arg1	1,4					164:166	1,4	164:166	1,3;1,4	160:166	In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications.
25661466	1	39	theme	downstream	182:191	arg1	implications					193:204	downstream implications	182:204	downstream implications for human health, animal feed and biofuel applications	182:259	In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications.
25661466	8	40	theme	H	1327:1327	arg1	families					1334:1341	the Cellulose synthase-like (Csl) F and H gene families	1287:1341	the Cellulose synthase-like (Csl) F and H gene families	1287:1341	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	8	41	dep	-β-glucan	1251:1259	arg1	1,3					1243:1245	1,3	1243:1245	1,3;1,4	1243:1249	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	6	42	theme	transmission	1037:1048	arg1	microscopy					1059:1068	fluorescence and transmission electron microscopy	1020:1068	microscopy	1059:1068	The distribution of (1,3;1,4)-β-glucan was examined using a specific antibody and observed with fluorescence and transmission electron microscopy.
25661466	6	43	theme	fluorescence	1020:1031	arg1	microscopy					1059:1068	fluorescence and transmission electron microscopy	1020:1068	microscopy	1059:1068	The distribution of (1,3;1,4)-β-glucan was examined using a specific antibody and observed with fluorescence and transmission electron microscopy.
25661466	3	44	theme	-β-glucan	425:433	arg1	present					435:441	(1,3;1,4)-β-glucan present	416:441	(1,3;1,4)-β-glucan present	416:441	Here, the amount of (1,3;1,4)-β-glucan present in sorghum tissues was measured using a Megazyme assay.
25661466	1	45	theme	soluble	125:131	arg1	-β-glucan					168:176	(1,3;1,4)-β-glucan	159:176	(1,3;1,4)-β-glucan	159:176	In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications.
25661466	1	45	theme	soluble	125:131	arg1	polysaccharides					133:147	soluble polysaccharides	125:147	soluble polysaccharides including (1,3;1,4)-β-glucan	125:176	In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications.
25661466	3	46	from	amount	406:411	arg1	tissues					454:460	sorghum tissues	446:460	sorghum tissues	446:460	Here, the amount of (1,3;1,4)-β-glucan present in sorghum tissues was measured using a Megazyme assay.
25661466	2	47	theme	bicolor	270:276	arg1	Moench					283:288	Sorghum bicolor (L.) Moench	262:288	Sorghum bicolor (L.) Moench	262:288	Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides.
25661466	2	47	theme	bicolor	270:276	arg1	crop					305:308	a versatile crop	293:308	a versatile crop	293:308	Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides.
25661466	5	48	theme	fine	657:660	arg1	structure					662:670	The fine structure	653:670	The fine structure	653:670	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	7	49	theme	spatial	1163:1169	arg1	factors					1171:1177	temporal and spatial factors	1150:1177	temporal and spatial factors	1150:1177	Micrographs showed a variable distribution of (1,3;1,4)-β-glucan influenced by temporal and spatial factors.
25661466	4	50	from	present	521:527	arg1	grain					536:540	the grain	532:540	the grain	532:540	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	1	51	dep	-β-glucan	168:176	arg1	1,3					160:162	1,3	160:162	1,3;1,4	160:166	In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications.
25661466	6	52	theme	specific	984:991	arg1	antibody					993:1000	a specific antibody	982:1000	a specific antibody	982:1000	The distribution of (1,3;1,4)-β-glucan was examined using a specific antibody and observed with fluorescence and transmission electron microscopy.
25661466	1	53	contain	has	178:180	arg2	implications					193:204	downstream implications	182:204	downstream implications for human health, animal feed and biofuel applications	182:259	In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications.
25661466	1	53	contain	has	178:180	arg1	presence					113:120	the presence	109:120	the presence of soluble polysaccharides including (1,3;1,4)-β-glucan	109:176	In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications.
25661466	9	54	theme	quantitative	1448:1459	arg1	reaction					1478:1485	real-time quantitative polymerase chain reaction	1438:1485	real-time quantitative polymerase chain reaction	1438:1485	Transcript profiling of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, CslF6 transcripts dominated.
25661466	4	55	located	present	521:527	arg1	grain					536:540	the grain	532:540	the grain	532:540	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	4	55	located	present	521:527	arg2	amounts					508:514	Very low amounts	499:514	Very low amounts	499:514	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	9	56	theme	chain	1472:1476	arg1	reaction					1478:1485	real-time quantitative polymerase chain reaction	1438:1485	real-time quantitative polymerase chain reaction	1438:1485	Transcript profiling of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, CslF6 transcripts dominated.
25661466	5	57	from	2.6-3:1	842:848	arg1	grain					857:861	the grain	853:861	the grain	853:861	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	7	58	dep	1,3	1118:1120	arg1	1,4					1122:1124	1,4	1122:1124	1,3;1,4	1118:1124	Micrographs showed a variable distribution of (1,3;1,4)-β-glucan influenced by temporal and spatial factors.
25661466	5	59	theme	-β-glucan	684:692	arg1	structure					662:670	The fine structure	653:670	The fine structure	653:670	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	4	60	theme	%	560:560	arg1	%					566:566	0.16%-0.27%	556:566	0.16%-0.27% (w/w)	556:572	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	4	60	theme	%	560:560	arg1	w/w					569:571	w/w	569:571	w/w	569:571	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	8	61	theme	-β-glucan	1251:1259	arg1	synthesis					1229:1237	the synthesis	1225:1237	the synthesis of (1,3;1,4)-β-glucan	1225:1259	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	8	62	theme	other	1264:1268	arg1	cereals					1270:1276	other cereals	1264:1276	other cereals	1264:1276	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	0	63	theme	Sorghum	80:86	arg1	bicolor					88:94	Sorghum bicolor	80:94	Sorghum bicolor	80:94	Distribution, structure and biosynthetic gene families of (1,3;1,4)-β-glucan in Sorghum bicolor.
25661466	4	64	from	quantity	601:608	arg1	tissues					624:630	vegetative tissues	613:630	vegetative tissues	613:630	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	9	65	theme	other	1511:1515	arg1	cereals					1517:1523	other cereals	1511:1523	other cereals	1511:1523	Transcript profiling of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, CslF6 transcripts dominated.
25661466	4	66	theme	vegetative	613:622	arg1	tissues					624:630	vegetative tissues	613:630	vegetative tissues	613:630	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	5	67	theme	cellotriosyl	722:733	arg1	residues					754:761	cellotriosyl and cellotetraosyl residues	722:761	cellotriosyl and cellotetraosyl residues	722:761	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	7	68	theme	variable	1092:1099	arg1	distribution					1101:1112	a variable distribution	1090:1112	a variable distribution of (1,3;1,4)-β-glucan	1090:1134	Micrographs showed a variable distribution of (1,3;1,4)-β-glucan influenced by temporal and spatial factors.
25661466	2	69	theme	such	366:369	arg1	polysaccharides					379:393	such soluble polysaccharides	366:393	such soluble polysaccharides	366:393	Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides.
25661466	5	70	from	ratios	870:875	arg1	tissues					891:897	vegetative tissues	880:897	vegetative tissues	880:897	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	7	71	dep	showed	1083:1088	arg1	influenced					1136:1145	influenced	1136:1145	showed a variable distribution of (1,3;1,4)-β-glucan influenced by temporal and spatial factors	1083:1177	Micrographs showed a variable distribution of (1,3;1,4)-β-glucan influenced by temporal and spatial factors.
25661466	5	72	theme	cellotetraosyl	739:752	arg1	residues					754:761	cellotriosyl and cellotetraosyl residues	722:761	cellotriosyl and cellotetraosyl residues	722:761	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	8	73	theme	Cellulose	1291:1299	arg1	F					1321:1321	the Cellulose synthase-like (Csl) F	1287:1321	the Cellulose synthase-like (Csl) F	1287:1321	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	0	74	theme	gene	41:44	arg1	families					46:53	biosynthetic gene families	28:53	biosynthetic gene families	28:53	Distribution, structure and biosynthetic gene families of (1,3;1,4)-β-glucan in Sorghum bicolor.
25661466	1	75	theme	biofuel	240:246	arg1	applications					248:259	biofuel applications	240:259	biofuel applications	240:259	In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications.
25661466	2	76	theme	limited	325:331	arg1	reports					333:339	limited reports	325:339	limited reports regarding the content of such soluble polysaccharides	325:393	Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides.
25661466	2	77	theme	polysaccharides	379:393	arg1	content					355:361	the content	351:361	the content of such soluble polysaccharides	351:393	Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides.
25661466	4	78	attach	present	521:527	arg2	amounts					508:514	Very low amounts	499:514	Very low amounts	499:514	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	4	78	attach	present	521:527	arg1	grain					536:540	the grain	532:540	the grain	532:540	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	5	79	theme	high	780:783	arg1	HPLC					820:823	HPLC	820:823	HPLC	820:823	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	5	79	theme	high	780:783	arg1	chromatography					804:817	high performance liquid chromatography	780:817	high performance liquid chromatography (HPLC)	780:824	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	7	80	theme	temporal	1150:1157	arg1	factors					1171:1177	temporal and spatial factors	1150:1177	temporal and spatial factors	1150:1177	Micrographs showed a variable distribution of (1,3;1,4)-β-glucan influenced by temporal and spatial factors.
25661466	4	81	theme	low	504:506	arg1	amounts					508:514	Very low amounts	499:514	Very low amounts	499:514	Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w).
25661466	5	82	theme	liquid	797:802	arg1	HPLC					820:823	HPLC	820:823	HPLC	820:823	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	5	82	theme	liquid	797:802	arg1	chromatography					804:817	high performance liquid chromatography	780:817	high performance liquid chromatography (HPLC)	780:824	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	8	83	theme	gene	1329:1332	arg1	families					1334:1341	the Cellulose synthase-like (Csl) F and H gene families	1287:1341	the Cellulose synthase-like (Csl) F and H gene families	1287:1341	The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined.
25661466	5	84	dep	structure	662:670	arg1	denoted					698:704	denoted	698:704	denoted by the ratio of cellotriosyl and cellotetraosyl residues	698:761	The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1.
25661466	2	85	theme	versatile	295:303	arg1	Moench					283:288	Sorghum bicolor (L.) Moench	262:288	Sorghum bicolor (L.) Moench	262:288	Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides.
25661466	2	85	theme	versatile	295:303	arg1	crop					305:308	a versatile crop	293:308	a versatile crop	293:308	Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides.
28855133	8	0	theme	hydrophobic	1290:1300	arg1	matrices					1310:1317	hydrophobic polymer matrices	1290:1317	hydrophobic polymer matrices	1290:1317	Thus, The silylation of CNCs may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices, and as nano-adsorbers due to the presence of amino groups attached on the surface.
28855133	8	1	theme	amino	1365:1369	arg1	groups					1371:1376	amino groups	1365:1376	amino groups attached on the surface	1365:1400	Thus, The silylation of CNCs may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices, and as nano-adsorbers due to the presence of amino groups attached on the surface.
28855133	1	2	theme	Surface	94:100	arg1	functionalization					102:118	Surface functionalization	94:118	Surface functionalization of cellulose nanocrystals (CNCs)	94:151	Surface functionalization of cellulose nanocrystals (CNCs) is valuable option to tailor properties as well as increase opportunities for their application.
28855133	1	3	dep	option	165:170	arg1	increase					204:211	increase	204:211	to tailor properties as well as increase opportunities for their application	172:247	Surface functionalization of cellulose nanocrystals (CNCs) is valuable option to tailor properties as well as increase opportunities for their application.
28855133	1	3	dep	option	165:170	arg1	tailor					175:180	tailor	175:180	to tailor properties as well as increase opportunities for their application	172:247	Surface functionalization of cellulose nanocrystals (CNCs) is valuable option to tailor properties as well as increase opportunities for their application.
28855133	6	4	theme	APTES	985:989	arg1	exsistence					971:980	the exsistence	967:980	the exsistence of APTES onto the CNCs	967:1003	The EDX and XPS results confirmed the exsistence of APTES onto the CNCs.
28855133	4	5	theme	CNCs	727:730	arg1	surface					732:738	the CNCs surface	723:738	the CNCs surface	723:738	The chemical modification of the CNCs surface was confirmed by ATR-IR and NMR spectroscopy.
28855133	5	6	theme	crystalline	878:888	arg1	structure					890:898	crystalline structure	878:898	crystalline structure	878:898	Experiments conducted by AFM and XRD showed no significant change in the CNC dimensions and crystalline structure as a result of the modification.
28855133	2	7	theme	straightforward	408:422	arg1	method					424:429	a direct, simple and straightforward method	387:429	method	424:429	In this study, the surface of CNCs was functionalized with 3-aminopropyltriethoxysilane (APTES), without using hazardous solvents and by a direct, simple and straightforward method.
28855133	8	8	attach	attached	1378:1385	arg3	surface					1394:1400	the surface	1390:1400	the surface	1390:1400	Thus, The silylation of CNCs may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices, and as nano-adsorbers due to the presence of amino groups attached on the surface.
28855133	8	8	attach	attached	1378:1385	arg2	groups					1371:1376	amino groups	1365:1376	amino groups attached on the surface	1365:1400	Thus, The silylation of CNCs may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices, and as nano-adsorbers due to the presence of amino groups attached on the surface.
28855133	7	9	theme	modified	1139:1146	arg1	CNC					1148:1150	non-chemically modified CNC	1124:1150	non-chemically modified CNC	1124:1150	Silylated CNC exhibited good thermal stability and a greater amount of residual char was formed at 500 °C compared to non-chemically modified CNC.
28855133	0	10	theme	chemical	8:15	arg1	functionalization					17:33	Surface chemical functionalization	0:33	Surface chemical functionalization of cellulose nanocrystals by 3-aminopropyltriethoxysilane.	0:92	Surface chemical functionalization of cellulose nanocrystals by 3-aminopropyltriethoxysilane.
28855133	7	11	theme	residual	1077:1084	arg1	char					1086:1089	residual char	1077:1089	residual char	1077:1089	Silylated CNC exhibited good thermal stability and a greater amount of residual char was formed at 500 °C compared to non-chemically modified CNC.
28855133	1	12	theme	cellulose	123:131	arg1	nanocrystals					133:144	cellulose nanocrystals	123:144	cellulose nanocrystals (CNCs)	123:151	Surface functionalization of cellulose nanocrystals (CNCs) is valuable option to tailor properties as well as increase opportunities for their application.
28855133	1	12	theme	cellulose	123:131	arg1	CNCs					147:150	CNCs	147:150	CNCs	147:150	Surface functionalization of cellulose nanocrystals (CNCs) is valuable option to tailor properties as well as increase opportunities for their application.
28855133	2	13	theme	simple	397:402	arg1	method					424:429	a direct, simple and straightforward method	387:429	method	424:429	In this study, the surface of CNCs was functionalized with 3-aminopropyltriethoxysilane (APTES), without using hazardous solvents and by a direct, simple and straightforward method.
28855133	0	14	theme	Surface	0:6	arg1	functionalization					17:33	Surface chemical functionalization	0:33	Surface chemical functionalization of cellulose nanocrystals by 3-aminopropyltriethoxysilane.	0:92	Surface chemical functionalization of cellulose nanocrystals by 3-aminopropyltriethoxysilane.
28855133	7	15	theme	char	1086:1089	arg1	amount					1067:1072	a greater amount	1057:1072	a greater amount of residual char	1057:1089	Silylated CNC exhibited good thermal stability and a greater amount of residual char was formed at 500 °C compared to non-chemically modified CNC.
28855133	7	15	theme	char	1086:1089	arg1	char					1086:1089	residual char	1077:1089	residual char	1077:1089	Silylated CNC exhibited good thermal stability and a greater amount of residual char was formed at 500 °C compared to non-chemically modified CNC.
28855133	1	16	theme	nanocrystals	133:144	arg1	functionalization					102:118	Surface functionalization	94:118	Surface functionalization of cellulose nanocrystals (CNCs)	94:151	Surface functionalization of cellulose nanocrystals (CNCs) is valuable option to tailor properties as well as increase opportunities for their application.
28855133	3	17	attach	linked	566:571	arg2	hydrocarbon					539:549	the chain hydrocarbon	529:549	the chain hydrocarbon	529:549	APTES was firstly hydrolyzed in water and then adsorbed onto CNC through hydrogen bonds, finally the chain hydrocarbon was covalently linked to the surface of CNC through SiOC bonds which formed via the condensation reaction between hydroxyl and silanol groups.
28855133	3	17	attach	linked	566:571	arg1	surface					580:586	the surface	576:586	the surface of CNC	576:593	APTES was firstly hydrolyzed in water and then adsorbed onto CNC through hydrogen bonds, finally the chain hydrocarbon was covalently linked to the surface of CNC through SiOC bonds which formed via the condensation reaction between hydroxyl and silanol groups.
28855133	8	18	theme	polymer	1302:1308	arg1	matrices					1310:1317	hydrophobic polymer matrices	1290:1317	hydrophobic polymer matrices	1290:1317	Thus, The silylation of CNCs may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices, and as nano-adsorbers due to the presence of amino groups attached on the surface.
28855133	5	19	theme	significant	833:843	arg1	result					905:910	a result	903:910	a result of the modification	903:930	Experiments conducted by AFM and XRD showed no significant change in the CNC dimensions and crystalline structure as a result of the modification.
28855133	5	19	theme	significant	833:843	arg1	change					845:850	no significant change	830:850	no significant change	830:850	Experiments conducted by AFM and XRD showed no significant change in the CNC dimensions and crystalline structure as a result of the modification.
28855133	8	20	theme	groups	1371:1376	arg1	presence					1353:1360	the presence	1349:1360	the presence of amino groups attached on the surface	1349:1400	Thus, The silylation of CNCs may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices, and as nano-adsorbers due to the presence of amino groups attached on the surface.
28855133	4	21	theme	chemical	698:705	arg1	modification					707:718	The chemical modification	694:718	The chemical modification of the CNCs surface	694:738	The chemical modification of the CNCs surface was confirmed by ATR-IR and NMR spectroscopy.
28855133	0	22	theme	nanocrystals	48:59	arg1	functionalization					17:33	Surface chemical functionalization	0:33	Surface chemical functionalization of cellulose nanocrystals by 3-aminopropyltriethoxysilane.	0:92	Surface chemical functionalization of cellulose nanocrystals by 3-aminopropyltriethoxysilane.
28855133	5	23	theme	modification	919:930	arg1	result					905:910	a result	903:910	a result of the modification	903:930	Experiments conducted by AFM and XRD showed no significant change in the CNC dimensions and crystalline structure as a result of the modification.
28855133	5	23	theme	modification	919:930	arg1	change					845:850	no significant change	830:850	no significant change	830:850	Experiments conducted by AFM and XRD showed no significant change in the CNC dimensions and crystalline structure as a result of the modification.
28855133	3	24	theme	hydroxyl	665:672	arg1	groups					686:691	hydroxyl and silanol groups	665:691	groups	686:691	APTES was firstly hydrolyzed in water and then adsorbed onto CNC through hydrogen bonds, finally the chain hydrocarbon was covalently linked to the surface of CNC through SiOC bonds which formed via the condensation reaction between hydroxyl and silanol groups.
28855133	3	25	theme	SiOC	603:606	arg1	bonds					608:612	SiOC bonds	603:612	SiOC bonds which formed via the condensation reaction between hydroxyl and silanol groups	603:691	APTES was firstly hydrolyzed in water and then adsorbed onto CNC through hydrogen bonds, finally the chain hydrocarbon was covalently linked to the surface of CNC through SiOC bonds which formed via the condensation reaction between hydroxyl and silanol groups.
28855133	0	26	theme	cellulose	38:46	arg1	nanocrystals					48:59	cellulose nanocrystals	38:59	cellulose nanocrystals	38:59	Surface chemical functionalization of cellulose nanocrystals by 3-aminopropyltriethoxysilane.
28855133	7	27	theme	greater	1059:1065	arg1	amount					1067:1072	a greater amount	1057:1072	a greater amount of residual char	1057:1089	Silylated CNC exhibited good thermal stability and a greater amount of residual char was formed at 500 °C compared to non-chemically modified CNC.
28855133	7	27	theme	greater	1059:1065	arg1	char					1086:1089	residual char	1077:1089	residual char	1077:1089	Silylated CNC exhibited good thermal stability and a greater amount of residual char was formed at 500 °C compared to non-chemically modified CNC.
28855133	3	28	theme	condensation	635:646	arg1	reaction					648:655	the condensation reaction	631:655	the condensation reaction between hydroxyl and silanol groups	631:691	APTES was firstly hydrolyzed in water and then adsorbed onto CNC through hydrogen bonds, finally the chain hydrocarbon was covalently linked to the surface of CNC through SiOC bonds which formed via the condensation reaction between hydroxyl and silanol groups.
28855133	5	29	theme	CNC	859:861	arg1	dimensions					863:872	the CNC dimensions	855:872	the CNC dimensions	855:872	Experiments conducted by AFM and XRD showed no significant change in the CNC dimensions and crystalline structure as a result of the modification.
28855133	8	30	theme	composite	1208:1216	arg1	manufacturing					1218:1230	composite manufacturing	1208:1230	composite manufacturing	1208:1230	Thus, The silylation of CNCs may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices, and as nano-adsorbers due to the presence of amino groups attached on the surface.
28855133	6	31	theme	XPS	945:947	arg1	results					949:955	The EDX and XPS results	933:955	The EDX and XPS results	933:955	The EDX and XPS results confirmed the exsistence of APTES onto the CNCs.
28855133	4	32	theme	NMR	768:770	arg1	spectroscopy					772:783	NMR spectroscopy	768:783	NMR spectroscopy	768:783	The chemical modification of the CNCs surface was confirmed by ATR-IR and NMR spectroscopy.
28855133	3	33	theme	CNC	591:593	arg1	surface					580:586	the surface	576:586	the surface of CNC	576:593	APTES was firstly hydrolyzed in water and then adsorbed onto CNC through hydrogen bonds, finally the chain hydrocarbon was covalently linked to the surface of CNC through SiOC bonds which formed via the condensation reaction between hydroxyl and silanol groups.
28855133	3	34	theme	hydrogen	505:512	arg1	bonds					514:518	hydrogen bonds	505:518	hydrogen bonds	505:518	APTES was firstly hydrolyzed in water and then adsorbed onto CNC through hydrogen bonds, finally the chain hydrocarbon was covalently linked to the surface of CNC through SiOC bonds which formed via the condensation reaction between hydroxyl and silanol groups.
28855133	2	35	theme	hazardous	361:369	arg1	solvents					371:378	hazardous solvents	361:378	hazardous solvents	361:378	In this study, the surface of CNCs was functionalized with 3-aminopropyltriethoxysilane (APTES), without using hazardous solvents and by a direct, simple and straightforward method.
28855133	6	36	theme	EDX	937:939	arg1	results					949:955	The EDX and XPS results	933:955	The EDX and XPS results	933:955	The EDX and XPS results confirmed the exsistence of APTES onto the CNCs.
28855133	7	37	theme	good	1030:1033	arg1	stability					1043:1051	good thermal stability	1030:1051	good thermal stability	1030:1051	Silylated CNC exhibited good thermal stability and a greater amount of residual char was formed at 500 °C compared to non-chemically modified CNC.
28855133	2	38	theme	CNCs	280:283	arg1	surface					269:275	the surface	265:275	the surface of CNCs	265:283	In this study, the surface of CNCs was functionalized with 3-aminopropyltriethoxysilane (APTES), without using hazardous solvents and by a direct, simple and straightforward method.
28855133	7	39	theme	Silylated	1006:1014	arg1	CNC					1016:1018	Silylated CNC	1006:1018	Silylated CNC	1006:1018	Silylated CNC exhibited good thermal stability and a greater amount of residual char was formed at 500 °C compared to non-chemically modified CNC.
28855133	1	40	theme	valuable	156:163	arg1	option					165:170	valuable option	156:170	valuable option to tailor properties as well as increase opportunities for their application	156:247	Surface functionalization of cellulose nanocrystals (CNCs) is valuable option to tailor properties as well as increase opportunities for their application.
28855133	7	41	theme	thermal	1035:1041	arg1	stability					1043:1051	good thermal stability	1030:1051	good thermal stability	1030:1051	Silylated CNC exhibited good thermal stability and a greater amount of residual char was formed at 500 °C compared to non-chemically modified CNC.
28855133	8	42	from	dispersibility	1272:1285	arg1	matrices					1310:1317	hydrophobic polymer matrices	1290:1317	hydrophobic polymer matrices	1290:1317	Thus, The silylation of CNCs may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices, and as nano-adsorbers due to the presence of amino groups attached on the surface.
28855133	4	43	theme	surface	732:738	arg1	modification					707:718	The chemical modification	694:718	The chemical modification of the CNCs surface	694:738	The chemical modification of the CNCs surface was confirmed by ATR-IR and NMR spectroscopy.
28855133	8	44	theme	due	1342:1344	arg1	nano-adsorbers					1327:1340	nano-adsorbers	1327:1340	nano-adsorbers due to the presence of amino groups attached on the surface	1327:1400	Thus, The silylation of CNCs may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices, and as nano-adsorbers due to the presence of amino groups attached on the surface.
28855133	3	45	theme	silanol	678:684	arg1	groups					686:691	hydroxyl and silanol groups	665:691	groups	686:691	APTES was firstly hydrolyzed in water and then adsorbed onto CNC through hydrogen bonds, finally the chain hydrocarbon was covalently linked to the surface of CNC through SiOC bonds which formed via the condensation reaction between hydroxyl and silanol groups.
28855133	2	46	theme	direct	389:394	arg1	method					424:429	a direct, simple and straightforward method	387:429	method	424:429	In this study, the surface of CNCs was functionalized with 3-aminopropyltriethoxysilane (APTES), without using hazardous solvents and by a direct, simple and straightforward method.
28855133	8	47	theme	CNCs	1177:1180	arg1	silylation					1163:1172	The silylation	1159:1172	The silylation of CNCs	1159:1180	Thus, The silylation of CNCs may offer applications in composite manufacturing, where these nanoparticles have limited dispersibility in hydrophobic polymer matrices, and as nano-adsorbers due to the presence of amino groups attached on the surface.
28855133	3	48	theme	chain	533:537	arg1	hydrocarbon					539:549	the chain hydrocarbon	529:549	the chain hydrocarbon	529:549	APTES was firstly hydrolyzed in water and then adsorbed onto CNC through hydrogen bonds, finally the chain hydrocarbon was covalently linked to the surface of CNC through SiOC bonds which formed via the condensation reaction between hydroxyl and silanol groups.
28242333	1	0	theme	oxide	266:270	arg1	composite					272:280	chitosan/zinc oxide composite	252:280	chitosan/zinc oxide composite (CZC)	252:286	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	1	0	theme	oxide	266:270	arg1	CZC					283:285	CZC	283:285	CZC	283:285	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	7	1	theme	photocatalytic	1056:1069	arg1	reactions					1071:1079	the photocatalytic reactions	1052:1079	the photocatalytic reactions	1052:1079	The kinetic studies demonstrated that the photocatalytic reactions followed the pseudo-first-order model.
28242333	3	2	dep	irradiation	647:657	arg1	VI					634:635	VI	634:635	VI	634:635	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	9	3	theme	UV	1222:1223	arg1	light					1225:1229	UV light	1222:1229	UV light	1222:1229	The Cr(VI) reduction by ZCB and CZC under UV light is governed by adsorption coupled photocatalytic reduction.
28242333	0	4	theme	oxide	56:60	arg1	materials					62:70	zinc oxide materials	51:70	zinc oxide materials	51:70	Photo-reduction of Cr(VI) using chitosan supported zinc oxide materials.
28242333	1	5	theme	composite	272:280	arg1	reduction					138:146	the photocatalytic reduction	119:146	the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC)	119:286	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	4	6	theme	UV	727:728	arg1	light					730:734	UV light	727:734	UV light	727:734	The maximum reduction percentage of Cr(VI) using ZCB and CZC under UV light was obtained at 60min of irradiation time.
28242333	5	7	dep	percentage	800:809	arg1	compared					837:844	compared	837:844	compared to ZCB which in turn higher than ZnO under UV light irradiation	837:908	CZC possesses higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation.
28242333	5	8	theme	UV	889:890	arg1	irradiation					898:908	UV light irradiation	889:908	UV light irradiation	889:908	CZC possesses higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation.
28242333	4	9	theme	reduction	672:680	arg1	percentage					682:691	The maximum reduction percentage	660:691	The maximum reduction percentage of Cr(VI) using ZCB and CZC under UV light	660:734	The maximum reduction percentage of Cr(VI) using ZCB and CZC under UV light was obtained at 60min of irradiation time.
28242333	5	10	theme	%	818:818	arg1	Cr					820:821	99.8% Cr	814:821	99.8% Cr	814:821	CZC possesses higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation.
28242333	5	11	dep	higher	867:872	arg1	ZCB					849:851	ZCB	849:851	ZCB which in turn higher than ZnO under UV light irradiation	849:908	CZC possesses higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation.
28242333	7	12	theme	pseudo-first-order	1094:1111	arg1	model					1113:1117	the pseudo-first-order model	1090:1117	the pseudo-first-order model	1090:1117	The kinetic studies demonstrated that the photocatalytic reactions followed the pseudo-first-order model.
28242333	3	13	theme	Cr	631:632	arg1	parameters					494:503	various parameters	486:503	various parameters viz.	486:508	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	13	theme	Cr	631:632	arg1	concentration					614:626	initial concentration	606:626	initial concentration of Cr	606:632	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	5	14	theme	Cr	820:821	arg1	percentage					800:809	higher percentage	793:809	higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation	793:908	CZC possesses higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation.
28242333	1	15	theme	zinc	204:207	arg1	oxide					191:195	zinc oxide	186:195	zinc oxide (ZnO)	186:201	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	1	15	theme	zinc	204:207	arg1	oxide					209:213	zinc oxide	204:213	zinc oxide	204:213	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	4	16	theme	maximum	664:670	arg1	percentage					682:691	The maximum reduction percentage	660:691	The maximum reduction percentage of Cr(VI) using ZCB and CZC under UV light	660:734	The maximum reduction percentage of Cr(VI) using ZCB and CZC under UV light was obtained at 60min of irradiation time.
28242333	1	17	theme	hexavalent	151:160	arg1	ZCB					243:245	ZCB	243:245	ZCB	243:245	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	1	17	theme	hexavalent	151:160	arg1	chromium					162:169	hexavalent chromium	151:169	hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB)	151:246	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	1	18	theme	chromium	162:169	arg1	reduction					138:146	the photocatalytic reduction	119:146	the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC)	119:286	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	8	19	theme	ZCB	1154:1156	arg1	reusability					1124:1134	The reusability	1120:1134	The reusability of as-synthesized ZCB and CZC	1120:1164	The reusability of as-synthesized ZCB and CZC was assessed.
28242333	1	20	theme	UV	294:295	arg1	irradiation					303:313	UV light irradiation	294:313	UV light irradiation	294:313	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	2	21	theme	EPR	419:421	arg1	studies					423:429	EPR studies	419:429	EPR studies	419:429	The as-synthesized chitosan modified zinc oxide materials were characterized using FTIR, SEM, EDAX and EPR studies.
28242333	5	22	theme	higher	793:798	arg1	percentage					800:809	higher percentage	793:809	higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation	793:908	CZC possesses higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation.
28242333	9	23	theme	coupled	1257:1263	arg1	reduction					1280:1288	adsorption coupled photocatalytic reduction	1246:1288	adsorption coupled photocatalytic reduction	1246:1288	The Cr(VI) reduction by ZCB and CZC under UV light is governed by adsorption coupled photocatalytic reduction.
28242333	9	24	theme	photocatalytic	1265:1278	arg1	reduction					1280:1288	adsorption coupled photocatalytic reduction	1246:1288	adsorption coupled photocatalytic reduction	1246:1288	The Cr(VI) reduction by ZCB and CZC under UV light is governed by adsorption coupled photocatalytic reduction.
28242333	10	25	theme	industrial	1401:1410	arg1	area					1412:1415	a nearby industrial area	1392:1415	a nearby industrial area using photocatalytic method	1392:1443	The suitability of ZCB and CZC was checked by employing these catalysts with the effluent taken from a nearby industrial area using photocatalytic method.
28242333	1	26	theme	impregnated	215:225	arg1	beads					236:240	zinc oxide (ZnO), zinc oxide impregnated chitosan beads	186:240	zinc oxide (ZnO), zinc oxide impregnated chitosan beads	186:240	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	10	27	theme	CZC	1318:1320	arg1	suitability					1295:1305	The suitability	1291:1305	The suitability of ZCB and CZC	1291:1320	The suitability of ZCB and CZC was checked by employing these catalysts with the effluent taken from a nearby industrial area using photocatalytic method.
28242333	3	28	theme	various	486:492	arg1	parameters					494:503	various parameters	486:503	various parameters viz.	486:508	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	28	theme	various	486:492	arg1	time					523:526	irradiation time	511:526	irradiation time	511:526	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	28	theme	various	486:492	arg1	concentration					614:626	initial concentration	606:626	initial concentration of Cr	606:632	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	2	29	theme	zinc	353:356	arg1	materials					364:372	The as-synthesized chitosan modified zinc oxide materials	316:372	The as-synthesized chitosan modified zinc oxide materials	316:372	The as-synthesized chitosan modified zinc oxide materials were characterized using FTIR, SEM, EDAX and EPR studies.
28242333	0	30	theme	Cr	19:20	arg1	Photo-reduction					0:14	Photo-reduction	0:14	Photo-reduction of Cr(VI) using chitosan	0:39	Photo-reduction of Cr(VI) using chitosan supported zinc oxide materials.
28242333	1	31	theme	chitosan	227:234	arg1	beads					236:240	zinc oxide (ZnO), zinc oxide impregnated chitosan beads	186:240	zinc oxide (ZnO), zinc oxide impregnated chitosan beads	186:240	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	4	32	theme	time	773:776	arg1	60min					752:756	60min	752:756	60min of irradiation time	752:776	The maximum reduction percentage of Cr(VI) using ZCB and CZC under UV light was obtained at 60min of irradiation time.
28242333	1	33	theme	light	297:301	arg1	irradiation					303:313	UV light irradiation	294:313	UV light irradiation	294:313	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	5	34	theme	99.8	814:817	arg1	%					818:818	%	818:818	%	818:818	CZC possesses higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation.
28242333	2	35	theme	modified	344:351	arg1	materials					364:372	The as-synthesized chitosan modified zinc oxide materials	316:372	The as-synthesized chitosan modified zinc oxide materials	316:372	The as-synthesized chitosan modified zinc oxide materials were characterized using FTIR, SEM, EDAX and EPR studies.
28242333	10	36	theme	ZCB	1310:1312	arg1	suitability					1295:1305	The suitability	1291:1305	The suitability of ZCB and CZC	1291:1320	The suitability of ZCB and CZC was checked by employing these catalysts with the effluent taken from a nearby industrial area using photocatalytic method.
28242333	5	37	theme	light	892:896	arg1	irradiation					898:908	UV light irradiation	889:908	UV light irradiation	889:908	CZC possesses higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation.
28242333	8	38	theme	CZC	1162:1164	arg1	reusability					1124:1134	The reusability	1120:1134	The reusability of as-synthesized ZCB and CZC	1120:1164	The reusability of as-synthesized ZCB and CZC was assessed.
28242333	2	39	theme	chitosan	335:342	arg1	materials					364:372	The as-synthesized chitosan modified zinc oxide materials	316:372	The as-synthesized chitosan modified zinc oxide materials	316:372	The as-synthesized chitosan modified zinc oxide materials were characterized using FTIR, SEM, EDAX and EPR studies.
28242333	4	40	dep	Cr	696:697	arg1	VI					699:700	VI	699:700	VI	699:700	The maximum reduction percentage of Cr(VI) using ZCB and CZC under UV light was obtained at 60min of irradiation time.
28242333	1	41	dep	Cr	172:173	arg1	VI					175:176	VI	175:176	VI	175:176	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	5	42	contain	possesses	783:791	arg1	CZC					779:781	CZC	779:781	CZC	779:781	CZC possesses higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation.
28242333	5	42	contain	possesses	783:791	arg2	percentage					800:809	higher percentage	793:809	higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation	793:908	CZC possesses higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation.
28242333	2	43	theme	as-synthesized	320:333	arg1	materials					364:372	The as-synthesized chitosan modified zinc oxide materials	316:372	The as-synthesized chitosan modified zinc oxide materials	316:372	The as-synthesized chitosan modified zinc oxide materials were characterized using FTIR, SEM, EDAX and EPR studies.
28242333	3	44	theme	UV	644:645	arg1	irradiation					647:657	UV irradiation	644:657	UV irradiation	644:657	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	45	theme	irradiation	511:521	arg1	pH					553:554	pH	553:554	pH	553:554	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	45	theme	irradiation	511:521	arg1	concentration					557:569	concentration	557:569	concentration of H2O2	557:577	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	45	theme	irradiation	511:521	arg1	parameters					494:503	various parameters	486:503	various parameters viz.	486:508	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	45	theme	irradiation	511:521	arg1	co-ions					597:603	co-ions	597:603	co-ions	597:603	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	45	theme	irradiation	511:521	arg1	amount					533:538	the amount	529:538	the amount of catalyst	529:550	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	45	theme	irradiation	511:521	arg1	catalyst					543:550	catalyst	543:550	catalyst	543:550	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	45	theme	irradiation	511:521	arg1	time					523:526	irradiation time	511:526	irradiation time	511:526	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	45	theme	irradiation	511:521	arg1	intensity					586:594	light intensity	580:594	light intensity	580:594	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	6	46	theme	photo-reduction	927:941	arg1	kinetics					915:922	The kinetics	911:922	The kinetics of photo-reduction of Cr(VI)	911:951	The kinetics of photo-reduction of Cr(VI) is explained on the basis of Langmuir-Hinshelwood mechanism.
28242333	10	47	theme	photocatalytic	1423:1436	arg1	method					1438:1443	photocatalytic method	1423:1443	photocatalytic method	1423:1443	The suitability of ZCB and CZC was checked by employing these catalysts with the effluent taken from a nearby industrial area using photocatalytic method.
28242333	9	48	dep	Cr	1184:1185	arg1	reduction					1191:1199	reduction	1191:1199	The Cr(VI) reduction by ZCB and CZC under UV light	1180:1229	The Cr(VI) reduction by ZCB and CZC under UV light is governed by adsorption coupled photocatalytic reduction.
28242333	9	48	dep	Cr	1184:1185	arg1	VI					1187:1188	VI	1187:1188	VI	1187:1188	The Cr(VI) reduction by ZCB and CZC under UV light is governed by adsorption coupled photocatalytic reduction.
28242333	3	49	theme	light	580:584	arg1	time					523:526	irradiation time	511:526	irradiation time	511:526	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	49	theme	light	580:584	arg1	intensity					586:594	light intensity	580:594	light intensity	580:594	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	1	50	dep	chromium	162:169	arg1	Cr					172:173	Cr	172:173	Cr	172:173	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	10	51	theme	nearby	1394:1399	arg1	area					1412:1415	a nearby industrial area	1392:1415	a nearby industrial area using photocatalytic method	1392:1443	The suitability of ZCB and CZC was checked by employing these catalysts with the effluent taken from a nearby industrial area using photocatalytic method.
28242333	6	52	theme	mechanism	1003:1011	arg1	basis					973:977	the basis	969:977	the basis of Langmuir-Hinshelwood mechanism	969:1011	The kinetics of photo-reduction of Cr(VI) is explained on the basis of Langmuir-Hinshelwood mechanism.
28242333	4	53	theme	irradiation	761:771	arg1	time					773:776	irradiation time	761:776	irradiation time	761:776	The maximum reduction percentage of Cr(VI) using ZCB and CZC under UV light was obtained at 60min of irradiation time.
28242333	6	54	dep	Cr	946:947	arg1	VI					949:950	VI	949:950	VI	949:950	The kinetics of photo-reduction of Cr(VI) is explained on the basis of Langmuir-Hinshelwood mechanism.
28242333	3	55	theme	catalyst	543:550	arg1	catalyst					543:550	catalyst	543:550	catalyst	543:550	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	55	theme	catalyst	543:550	arg1	time					523:526	irradiation time	511:526	irradiation time	511:526	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	55	theme	catalyst	543:550	arg1	amount					533:538	the amount	529:538	the amount of catalyst	529:550	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	6	56	theme	Cr	946:947	arg1	photo-reduction					927:941	photo-reduction	927:941	photo-reduction of Cr(VI)	927:951	The kinetics of photo-reduction of Cr(VI) is explained on the basis of Langmuir-Hinshelwood mechanism.
28242333	6	57	theme	Langmuir-Hinshelwood	982:1001	arg1	mechanism					1003:1011	Langmuir-Hinshelwood mechanism	982:1011	Langmuir-Hinshelwood mechanism	982:1011	The kinetics of photo-reduction of Cr(VI) is explained on the basis of Langmuir-Hinshelwood mechanism.
28242333	3	58	theme	H2O2	574:577	arg1	concentration					557:569	concentration	557:569	concentration of H2O2	557:577	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	58	theme	H2O2	574:577	arg1	time					523:526	irradiation time	511:526	irradiation time	511:526	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	0	59	dep	Cr	19:20	arg1	VI					22:23	VI	22:23	VI	22:23	Photo-reduction of Cr(VI) using chitosan supported zinc oxide materials.
28242333	8	60	theme	as-synthesized	1139:1152	arg1	ZCB					1154:1156	as-synthesized ZCB	1139:1156	as-synthesized ZCB	1139:1156	The reusability of as-synthesized ZCB and CZC was assessed.
28242333	1	61	theme	zinc	186:189	arg1	oxide					191:195	zinc oxide	186:195	zinc oxide (ZnO)	186:201	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	1	61	theme	zinc	186:189	arg1	oxide					209:213	zinc oxide	204:213	zinc oxide	204:213	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	1	61	theme	zinc	186:189	arg1	ZnO					198:200	ZnO	198:200	ZnO	198:200	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	0	62	theme	zinc	51:54	arg1	materials					62:70	zinc oxide materials	51:70	zinc oxide materials	51:70	Photo-reduction of Cr(VI) using chitosan supported zinc oxide materials.
28242333	4	63	theme	Cr	696:697	arg1	percentage					682:691	The maximum reduction percentage	660:691	The maximum reduction percentage of Cr(VI) using ZCB and CZC under UV light	660:734	The maximum reduction percentage of Cr(VI) using ZCB and CZC under UV light was obtained at 60min of irradiation time.
28242333	5	64	dep	compared	837:844	arg1	VI					823:824	VI	823:824	VI	823:824	CZC possesses higher percentage of 99.8% Cr(VI) reduction compared to ZCB which in turn higher than ZnO under UV light irradiation.
28242333	3	65	theme	Photocatalytic	432:445	arg1	experiments					447:457	Photocatalytic experiments	432:457	Photocatalytic experiments	432:457	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	9	66	theme	adsorption	1246:1255	arg1	reduction					1280:1288	adsorption coupled photocatalytic reduction	1246:1288	adsorption coupled photocatalytic reduction	1246:1288	The Cr(VI) reduction by ZCB and CZC under UV light is governed by adsorption coupled photocatalytic reduction.
28242333	1	67	theme	oxide	191:195	arg1	beads					236:240	zinc oxide (ZnO), zinc oxide impregnated chitosan beads	186:240	zinc oxide (ZnO), zinc oxide impregnated chitosan beads	186:240	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	1	68	theme	chitosan/zinc	252:264	arg1	composite					272:280	chitosan/zinc oxide composite	252:280	chitosan/zinc oxide composite (CZC)	252:286	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	1	68	theme	chitosan/zinc	252:264	arg1	CZC					283:285	CZC	283:285	CZC	283:285	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	2	69	theme	oxide	358:362	arg1	materials					364:372	The as-synthesized chitosan modified zinc oxide materials	316:372	The as-synthesized chitosan modified zinc oxide materials	316:372	The as-synthesized chitosan modified zinc oxide materials were characterized using FTIR, SEM, EDAX and EPR studies.
28242333	3	70	theme	initial	606:612	arg1	parameters					494:503	various parameters	486:503	various parameters viz.	486:508	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	3	70	theme	initial	606:612	arg1	concentration					614:626	initial concentration	606:626	initial concentration of Cr	606:632	Photocatalytic experiments were conducted to optimize various parameters viz., irradiation time, the amount of catalyst, pH, concentration of H2O2, light intensity, co-ions, initial concentration of Cr(VI) under UV irradiation.
28242333	1	71	theme	photocatalytic	123:136	arg1	reduction					138:146	the photocatalytic reduction	119:146	the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC)	119:286	Investigations were made in order to evaluate the photocatalytic reduction of hexavalent chromium (Cr(VI)) using zinc oxide (ZnO), zinc oxide impregnated chitosan beads (ZCB) and chitosan/zinc oxide composite (CZC) under UV light irradiation.
28242333	7	72	theme	kinetic	1018:1024	arg1	studies					1026:1032	The kinetic studies	1014:1032	The kinetic studies	1014:1032	The kinetic studies demonstrated that the photocatalytic reactions followed the pseudo-first-order model.
25164347	0	0	theme	airlift	94:100	arg1	photobioreactor					114:128	an airlift circulation photobioreactor	91:128	an airlift circulation photobioreactor	91:128	Chlorella pyrenoidosa cultivation using anaerobic digested starch processing wastewater in an airlift circulation photobioreactor.
25164347	1	1	theme	sludge	384:389	arg1	reactor					402:408	an upflow anaerobic sludge bed (UASB) reactor	364:408	an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment	364:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	2	2	theme	different	608:616	arg1	seasons					618:624	different seasons	608:624	different seasons	608:624	Chlorella pyrenoidosa completely adapted to the digested SPW without any chemical additives, and it grew normally under a wide temperature range in different seasons.
25164347	2	3	from	range	599:603	arg1	seasons					618:624	different seasons	608:624	different seasons	608:624	Chlorella pyrenoidosa completely adapted to the digested SPW without any chemical additives, and it grew normally under a wide temperature range in different seasons.
25164347	1	4	theme	bed	391:393	arg1	reactor					402:408	an upflow anaerobic sludge bed (UASB) reactor	364:408	an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment	364:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	1	5	theme	anaerobic	188:196	arg1	processing					198:207	anaerobic processing	188:207	anaerobic processing for wastewater treatment	188:232	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	1	6	theme	dynamic	292:298	arg1	reactor					309:315	a dynamic membrane reactor	290:315	a dynamic membrane reactor	290:315	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	3	7	from	microorganism	666:678	arg1	photobioreactors					687:702	the photobioreactors	683:702	the photobioreactors	683:702	C. pyrenoidosa was always the dominant microorganism in the photobioreactors although bacteria and some wild type microalgae were observed.
25164347	3	8	theme	type	736:739	arg1	microalgae					741:750	some wild type microalgae	726:750	some wild type microalgae	726:750	C. pyrenoidosa was always the dominant microorganism in the photobioreactors although bacteria and some wild type microalgae were observed.
25164347	6	9	theme	biomass	1135:1141	arg1	production					1143:1152	microalgae biomass production	1124:1152	microalgae biomass production	1124:1152	The results demonstrate the successful integration of microalgae biomass production and anaerobic processing for wastewater treatment.
25164347	1	10	theme	membrane	300:307	arg1	reactor					309:315	a dynamic membrane reactor	290:315	a dynamic membrane reactor	290:315	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	0	11	theme	circulation	102:112	arg1	photobioreactor					114:128	an airlift circulation photobioreactor	91:128	an airlift circulation photobioreactor	91:128	Chlorella pyrenoidosa cultivation using anaerobic digested starch processing wastewater in an airlift circulation photobioreactor.
25164347	1	12	theme	UASB	396:399	arg1	reactor					402:408	an upflow anaerobic sludge bed (UASB) reactor	364:408	an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment	364:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	1	13	with	combination	347:357	arg1	reactor					402:408	an upflow anaerobic sludge bed (UASB) reactor	364:408	an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment	364:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	6	14	theme	wastewater	1183:1192	arg1	treatment					1194:1202	wastewater treatment	1183:1202	wastewater treatment	1183:1202	The results demonstrate the successful integration of microalgae biomass production and anaerobic processing for wastewater treatment.
25164347	6	15	theme	successful	1098:1107	arg1	integration					1109:1119	the successful integration	1094:1119	the successful integration of microalgae biomass production	1094:1152	The results demonstrate the successful integration of microalgae biomass production and anaerobic processing for wastewater treatment.
25164347	4	16	theme	0.37gL	954:959	arg1	-1					966:967	-1	966:967	-1	966:967	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	16	theme	0.37gL	954:959	arg1	d					964:964	0.37gL(-1)d	954:964	0.37gL(-1)d(-1)	954:968	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	0	17	theme	pyrenoidosa	10:20	arg1	cultivation					22:32	Chlorella pyrenoidosa cultivation	0:32	Chlorella pyrenoidosa cultivation	0:32	Chlorella pyrenoidosa cultivation using anaerobic digested starch processing wastewater in an airlift circulation photobioreactor.
25164347	4	18	theme	COD	892:894	arg1	TP					921:922	TP	921:922	TP	921:922	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	18	theme	COD	892:894	arg1	%					887:887	65.99%	882:887	65.99% of COD	882:894	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	18	theme	COD	892:894	arg1	TN					907:908	TN	907:908	TN	907:908	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	18	theme	COD	892:894	arg1	COD					892:894	COD	892:894	COD	892:894	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	18	theme	COD	892:894	arg1	d					964:964	0.37gL(-1)d	954:964	0.37gL(-1)d(-1)	954:968	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	18	theme	COD	892:894	arg1	%					916:916	96.97%	911:916	96.97% of TP	911:922	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	18	theme	COD	892:894	arg1	productivity					938:949	a biomass productivity	928:949	a biomass productivity of 0.37gL(-1)d(-1)	928:968	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	18	theme	COD	892:894	arg1	%					902:902	83.06%	897:902	83.06% of TN	897:908	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	18	theme	COD	892:894	arg1	-1					966:967	-1	966:967	-1	966:967	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	6	19	theme	anaerobic	1158:1166	arg1	processing					1168:1177	anaerobic processing	1158:1177	anaerobic processing	1158:1177	The results demonstrate the successful integration of microalgae biomass production and anaerobic processing for wastewater treatment.
25164347	4	20	theme	TN	907:908	arg1	TP					921:922	TP	921:922	TP	921:922	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	20	theme	TN	907:908	arg1	%					887:887	65.99%	882:887	65.99% of COD	882:894	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	20	theme	TN	907:908	arg1	TN					907:908	TN	907:908	TN	907:908	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	20	theme	TN	907:908	arg1	COD					892:894	COD	892:894	COD	892:894	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	20	theme	TN	907:908	arg1	d					964:964	0.37gL(-1)d	954:964	0.37gL(-1)d(-1)	954:968	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	20	theme	TN	907:908	arg1	%					916:916	96.97%	911:916	96.97% of TP	911:922	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	20	theme	TN	907:908	arg1	productivity					938:949	a biomass productivity	928:949	a biomass productivity of 0.37gL(-1)d(-1)	928:968	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	20	theme	TN	907:908	arg1	%					902:902	83.06%	897:902	83.06% of TN	897:908	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	20	theme	TN	907:908	arg1	-1					966:967	-1	966:967	-1	966:967	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	1	21	theme	wastewater	213:222	arg1	treatment					224:232	wastewater treatment	213:232	wastewater treatment	213:232	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	0	22	theme	Chlorella	0:8	arg1	cultivation					22:32	Chlorella pyrenoidosa cultivation	0:32	Chlorella pyrenoidosa cultivation	0:32	Chlorella pyrenoidosa cultivation using anaerobic digested starch processing wastewater in an airlift circulation photobioreactor.
25164347	5	23	theme	lipid	1020:1024	arg1	contents					1026:1033	lipid contents	1020:1033	lipid contents	1020:1033	Temperature fluctuation significantly influenced lipid contents and FAMEs compositions in biomass.
25164347	1	24	theme	microalgae	321:330	arg1	cultivation					332:342	microalgae cultivation	321:342	microalgae cultivation	321:342	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	2	25	theme	wide	582:585	arg1	range					599:603	a wide temperature range	580:603	a wide temperature range in different seasons	580:624	Chlorella pyrenoidosa completely adapted to the digested SPW without any chemical additives, and it grew normally under a wide temperature range in different seasons.
25164347	6	26	theme	production	1143:1152	arg1	integration					1109:1119	the successful integration	1094:1119	the successful integration of microalgae biomass production	1094:1152	The results demonstrate the successful integration of microalgae biomass production and anaerobic processing for wastewater treatment.
25164347	6	26	theme	production	1143:1152	arg1	processing					1168:1177	anaerobic processing	1158:1177	anaerobic processing	1158:1177	The results demonstrate the successful integration of microalgae biomass production and anaerobic processing for wastewater treatment.
25164347	5	27	theme	Temperature	971:981	arg1	fluctuation					983:993	Temperature fluctuation	971:993	Temperature fluctuation	971:993	Temperature fluctuation significantly influenced lipid contents and FAMEs compositions in biomass.
25164347	4	28	theme	d	964:964	arg1	TP					921:922	TP	921:922	TP	921:922	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	28	theme	d	964:964	arg1	%					887:887	65.99%	882:887	65.99% of COD	882:894	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	28	theme	d	964:964	arg1	TN					907:908	TN	907:908	TN	907:908	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	28	theme	d	964:964	arg1	COD					892:894	COD	892:894	COD	892:894	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	28	theme	d	964:964	arg1	d					964:964	0.37gL(-1)d	954:964	0.37gL(-1)d(-1)	954:968	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	28	theme	d	964:964	arg1	%					916:916	96.97%	911:916	96.97% of TP	911:922	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	28	theme	d	964:964	arg1	productivity					938:949	a biomass productivity	928:949	a biomass productivity of 0.37gL(-1)d(-1)	928:968	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	28	theme	d	964:964	arg1	%					902:902	83.06%	897:902	83.06% of TN	897:908	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	28	theme	d	964:964	arg1	-1					966:967	-1	966:967	-1	966:967	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	2	29	theme	Chlorella	460:468	arg1	pyrenoidosa					470:480	Chlorella pyrenoidosa	460:480	Chlorella pyrenoidosa	460:480	Chlorella pyrenoidosa completely adapted to the digested SPW without any chemical additives, and it grew normally under a wide temperature range in different seasons.
25164347	1	30	from	reactor	309:315	arg1	combination					347:357	combination	347:357	combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment	347:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	1	31	theme	starch	414:419	arg1	treatment					449:457	starch processing wastewater (SPW) treatment	414:457	starch processing wastewater (SPW) treatment	414:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	4	32	theme	pollutants	794:803	arg1	removal					805:811	pollutants removal	794:811	pollutants removal	794:811	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	0	33	theme	digested	50:57	arg1	wastewater					77:86	anaerobic digested starch processing wastewater	40:86	anaerobic digested starch processing wastewater in an airlift circulation photobioreactor	40:128	Chlorella pyrenoidosa cultivation using anaerobic digested starch processing wastewater in an airlift circulation photobioreactor.
25164347	1	34	theme	processing	421:430	arg1	treatment					449:457	starch processing wastewater (SPW) treatment	414:457	starch processing wastewater (SPW) treatment	414:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	3	35	theme	C.	627:628	arg1	microorganism					666:678	the dominant microorganism	653:678	the dominant microorganism in the photobioreactors	653:702	C. pyrenoidosa was always the dominant microorganism in the photobioreactors although bacteria and some wild type microalgae were observed.
25164347	3	35	theme	C.	627:628	arg1	pyrenoidosa					630:640	C. pyrenoidosa	627:640	C. pyrenoidosa	627:640	C. pyrenoidosa was always the dominant microorganism in the photobioreactors although bacteria and some wild type microalgae were observed.
25164347	0	36	theme	anaerobic	40:48	arg1	wastewater					77:86	anaerobic digested starch processing wastewater	40:86	anaerobic digested starch processing wastewater in an airlift circulation photobioreactor	40:128	Chlorella pyrenoidosa cultivation using anaerobic digested starch processing wastewater in an airlift circulation photobioreactor.
25164347	2	37	theme	digested	508:515	arg1	SPW					517:519	the digested SPW	504:519	the digested SPW	504:519	Chlorella pyrenoidosa completely adapted to the digested SPW without any chemical additives, and it grew normally under a wide temperature range in different seasons.
25164347	1	38	theme	wastewater	432:441	arg1	treatment					449:457	starch processing wastewater (SPW) treatment	414:457	starch processing wastewater (SPW) treatment	414:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	1	39	used	utilized	238:245	arg2	we					235:236	we	235:236	we	235:236	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	0	40	theme	processing	66:75	arg1	wastewater					77:86	anaerobic digested starch processing wastewater	40:86	anaerobic digested starch processing wastewater in an airlift circulation photobioreactor	40:128	Chlorella pyrenoidosa cultivation using anaerobic digested starch processing wastewater in an airlift circulation photobioreactor.
25164347	1	41	from	photobioreactor	270:284	arg1	combination					347:357	combination	347:357	combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment	347:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	0	42	from	wastewater	77:86	arg1	photobioreactor					114:128	an airlift circulation photobioreactor	91:128	an airlift circulation photobioreactor	91:128	Chlorella pyrenoidosa cultivation using anaerobic digested starch processing wastewater in an airlift circulation photobioreactor.
25164347	6	43	theme	microalgae	1124:1133	arg1	production					1143:1152	microalgae biomass production	1124:1152	microalgae biomass production	1124:1152	The results demonstrate the successful integration of microalgae biomass production and anaerobic processing for wastewater treatment.
25164347	0	44	theme	starch	59:64	arg1	wastewater					77:86	anaerobic digested starch processing wastewater	40:86	anaerobic digested starch processing wastewater in an airlift circulation photobioreactor	40:128	Chlorella pyrenoidosa cultivation using anaerobic digested starch processing wastewater in an airlift circulation photobioreactor.
25164347	4	45	theme	TP	921:922	arg1	TP					921:922	TP	921:922	TP	921:922	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	45	theme	TP	921:922	arg1	%					887:887	65.99%	882:887	65.99% of COD	882:894	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	45	theme	TP	921:922	arg1	TN					907:908	TN	907:908	TN	907:908	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	45	theme	TP	921:922	arg1	COD					892:894	COD	892:894	COD	892:894	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	45	theme	TP	921:922	arg1	d					964:964	0.37gL(-1)d	954:964	0.37gL(-1)d(-1)	954:968	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	45	theme	TP	921:922	arg1	%					916:916	96.97%	911:916	96.97% of TP	911:922	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	45	theme	TP	921:922	arg1	productivity					938:949	a biomass productivity	928:949	a biomass productivity of 0.37gL(-1)d(-1)	928:968	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	45	theme	TP	921:922	arg1	%					902:902	83.06%	897:902	83.06% of TN	897:908	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	4	45	theme	TP	921:922	arg1	-1					966:967	-1	966:967	-1	966:967	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	1	46	theme	SPW	444:446	arg1	treatment					449:457	starch processing wastewater (SPW) treatment	414:457	starch processing wastewater (SPW) treatment	414:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	1	47	theme	upflow	367:372	arg1	reactor					402:408	an upflow anaerobic sludge bed (UASB) reactor	364:408	an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment	364:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	3	48	theme	wild	731:734	arg1	type					736:739	some wild type	726:739	some wild type microalgae	726:750	C. pyrenoidosa was always the dominant microorganism in the photobioreactors although bacteria and some wild type microalgae were observed.
25164347	4	49	theme	biomass	775:781	arg1	growth					783:788	Optimal biomass growth	767:788	Optimal biomass growth	767:788	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	1	50	theme	airlift	250:256	arg1	photobioreactor					270:284	an airlift circulation photobioreactor	247:284	an airlift circulation photobioreactor	247:284	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	2	51	theme	temperature	587:597	arg1	range					599:603	a wide temperature range	580:603	a wide temperature range in different seasons	580:624	Chlorella pyrenoidosa completely adapted to the digested SPW without any chemical additives, and it grew normally under a wide temperature range in different seasons.
25164347	3	52	theme	dominant	657:664	arg1	microorganism					666:678	the dominant microorganism	653:678	the dominant microorganism in the photobioreactors	653:702	C. pyrenoidosa was always the dominant microorganism in the photobioreactors although bacteria and some wild type microalgae were observed.
25164347	3	52	theme	dominant	657:664	arg1	pyrenoidosa					630:640	C. pyrenoidosa	627:640	C. pyrenoidosa	627:640	C. pyrenoidosa was always the dominant microorganism in the photobioreactors although bacteria and some wild type microalgae were observed.
25164347	4	53	theme	Optimal	767:773	arg1	growth					783:788	Optimal biomass growth	767:788	Optimal biomass growth	767:788	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	1	54	theme	circulation	258:268	arg1	photobioreactor					270:284	an airlift circulation photobioreactor	247:284	an airlift circulation photobioreactor	247:284	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	5	55	theme	FAMEs	1039:1043	arg1	compositions					1045:1056	FAMEs compositions	1039:1056	FAMEs compositions	1039:1056	Temperature fluctuation significantly influenced lipid contents and FAMEs compositions in biomass.
25164347	1	56	theme	microalgae	161:170	arg1	cultivation					172:182	microalgae cultivation	161:182	microalgae cultivation	161:182	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	2	57	theme	chemical	533:540	arg1	additives					542:550	any chemical additives	529:550	any chemical additives	529:550	Chlorella pyrenoidosa completely adapted to the digested SPW without any chemical additives, and it grew normally under a wide temperature range in different seasons.
25164347	1	58	theme	anaerobic	374:382	arg1	reactor					402:408	an upflow anaerobic sludge bed (UASB) reactor	364:408	an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment	364:457	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	4	59	from	35	850:851	arg1	summer					865:870	summer	865:870	summer	865:870	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
25164347	1	60	theme	cultivation	172:182	arg1	integration					146:156	the integration	142:156	the integration of microalgae cultivation	142:182	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	1	60	theme	cultivation	172:182	arg1	processing					198:207	anaerobic processing	188:207	anaerobic processing for wastewater treatment	188:232	To explore the integration of microalgae cultivation and anaerobic processing for wastewater treatment, we utilized an airlift circulation photobioreactor and a dynamic membrane reactor for microalgae cultivation in combination with an upflow anaerobic sludge bed (UASB) reactor for starch processing wastewater (SPW) treatment.
25164347	4	61	theme	biomass	930:936	arg1	productivity					938:949	a biomass productivity	928:949	a biomass productivity of 0.37gL(-1)d(-1)	928:968	Optimal biomass growth and pollutants removal was achieved at temperatures between 35 and 38°C in summer, removing 65.99% of COD, 83.06% of TN, 96.97% of TP and a biomass productivity of 0.37gL(-1)d(-1).
27274950	8	0	theme	secondary	1652:1660	arg1	structure					1662:1670	the secondary structure	1648:1670	the secondary structure	1648:1670	During regeneration, these interactions were reestablished to reform partially the secondary structure.
27274950	6	1	theme	β-turn	1354:1359	arg1	form					1372:1375	relatively lower α-helix, higher β-turn and random form	1321:1375	relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool	1321:1418	Results obtained with this method agree well with those by XRD, namely they indicate that although KER retains its second structure when incorporated into the [CEL+CS] composites, it has relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool.
27274950	0	2	theme	Recyclable	64:73	arg1	Synthesis					75:83	Synthesis	75:83	Synthesis	75:83	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	2	theme	Recyclable	64:73	arg1	Materials					42:50	Cellulose, Chitosan and Keratin Composite Materials	0:50	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.	0:113	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	3	3	theme	SEM	545:547	arg1	results					549:555	FTIR, XRD, 13C CP-MAS NMR and SEM results	515:555	FTIR, XRD, 13C CP-MAS NMR and SEM results	515:555	FTIR, XRD, 13C CP-MAS NMR and SEM results confirm that KER, CS and CEL remain chemically intact and distributed homogeneously in the composites.
27274950	11	4	theme	[CS/CEL+KER	2087:2097	arg1	composites					2100:2109	the [CS/CEL+KER] composites	2083:2109	the [CS/CEL+KER] composites	2083:2109	As anticipated, results of tensile strength and TGA confirm that adding CEL or CS into KER substantially increase the mechanical strength and thermal stability of the [CS/CEL+KER] composites.
27274950	5	5	theme	amide	1036:1040	arg1	bands					1042:1046	the amide bands	1032:1046	the amide bands	1032:1046	A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information.
27274950	1	6	theme	wider	292:296	arg1	utilization					298:308	wider utilization	292:308	wider utilization	292:308	A method was developed in which cellulose (CEL) and/or chitosan (CS) were added to keratin (KER) to enable [CEL/CS+KER] composites formed to have better mechanical strength and wider utilization.
27274950	7	7	from	inter-	1473:1478	arg1	KER					1509:1511	KER	1509:1511	KER	1509:1511	It seems that during dissolution by [BMIm+Cl-], the inter- and intramolecular forces in KER were broken thereby destroying its secondary structure.
27274950	4	8	theme	parameters	914:923	arg1	choice					904:909	the choice	900:909	the choice of parameters selected for curve fitting	900:950	We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting.
27274950	5	9	theme	bands	1042:1046	arg1	PLSR					1023:1026	PLSR	1023:1026	PLSR	1023:1026	A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information.
27274950	5	9	theme	bands	1042:1046	arg1	analysis					1013:1020	the partial least squares regression analysis	976:1020	the partial least squares regression analysis (PLSR) of the amide bands	976:1046	A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information.
27274950	4	10	theme	secondary	789:797	arg1	structure					799:807	secondary structure	789:807	secondary structure of proteins	789:819	We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting.
27274950	2	11	theme	[BMIm+Cl-	433:441	arg1	majority					421:428	the majority	417:428	the majority of [BMIm+Cl-] used (at least 88%)	417:462	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	5	12	theme	new	955:957	arg1	method					959:964	A new method	953:964	A new method	953:964	A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information.
27274950	4	13	theme	used	704:707	arg1	method					709:714	the widely used method	693:714	the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins	693:819	We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting.
27274950	4	13	theme	used	704:707	arg1	subjective					835:844	subjective	835:844	subjective	835:844	We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting.
27274950	11	14	theme	thermal	2062:2068	arg1	stability					2070:2078	thermal stability	2062:2078	thermal stability	2062:2078	As anticipated, results of tensile strength and TGA confirm that adding CEL or CS into KER substantially increase the mechanical strength and thermal stability of the [CS/CEL+KER] composites.
27274950	2	15	used	used	379:382	arg2	chloride					335:342	Butylmethylimmidazolium chloride	311:342	Butylmethylimmidazolium chloride ([BMIm+Cl-])	311:355	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	2	15	used	used	379:382	arg2	liquid					367:372	liquid	367:372	liquid	367:372	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	2	15	used	used	379:382	arg2	solvent					396:402	the sole solvent	387:402	the sole solvent	387:402	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	2	15	used	used	379:382	arg2	[BMIm+Cl-					345:353	[BMIm+Cl-	345:353	[BMIm+Cl-	345:353	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	11	16	theme	tensile	1947:1953	arg1	strength					1955:1962	tensile strength	1947:1962	tensile strength	1947:1962	As anticipated, results of tensile strength and TGA confirm that adding CEL or CS into KER substantially increase the mechanical strength and thermal stability of the [CS/CEL+KER] composites.
27274950	6	17	theme	native	1408:1413	arg1	wool					1415:1418	native wool	1408:1418	native wool	1408:1418	Results obtained with this method agree well with those by XRD, namely they indicate that although KER retains its second structure when incorporated into the [CEL+CS] composites, it has relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool.
27274950	6	18	theme	α-helix	1338:1344	arg1	form					1372:1375	relatively lower α-helix, higher β-turn and random form	1321:1375	relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool	1321:1418	Results obtained with this method agree well with those by XRD, namely they indicate that although KER retains its second structure when incorporated into the [CEL+CS] composites, it has relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool.
27274950	4	19	theme	proteins	812:819	arg1	structure					799:807	secondary structure	789:807	secondary structure of proteins	789:819	We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting.
27274950	6	20	theme	second	1249:1254	arg1	structure					1256:1264	its second structure	1245:1264	its second structure	1245:1264	Results obtained with this method agree well with those by XRD, namely they indicate that although KER retains its second structure when incorporated into the [CEL+CS] composites, it has relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool.
27274950	11	21	theme	TGA	1968:1970	arg1	results					1936:1942	results	1936:1942	results of tensile strength and TGA	1936:1970	As anticipated, results of tensile strength and TGA confirm that adding CEL or CS into KER substantially increase the mechanical strength and thermal stability of the [CS/CEL+KER] composites.
27274950	6	22	contain	has	1317:1319	arg2	form					1372:1375	relatively lower α-helix, higher β-turn and random form	1321:1375	relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool	1321:1418	Results obtained with this method agree well with those by XRD, namely they indicate that although KER retains its second structure when incorporated into the [CEL+CS] composites, it has relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool.
27274950	6	22	contain	has	1317:1319	arg1	it					1314:1315	it	1314:1315	it	1314:1315	Results obtained with this method agree well with those by XRD, namely they indicate that although KER retains its second structure when incorporated into the [CEL+CS] composites, it has relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool.
27274950	7	23	theme	secondary	1548:1556	arg1	structure					1558:1566	its secondary structure	1544:1566	its secondary structure	1544:1566	It seems that during dissolution by [BMIm+Cl-], the inter- and intramolecular forces in KER were broken thereby destroying its secondary structure.
27274950	9	24	theme	CEL	1708:1710	arg1	presence					1689:1696	the presence	1685:1696	the presence of either CEL or CS	1685:1716	However, in the presence of either CEL or CS, the chains seem to prefer the extended form thereby hindering reformation of the α-helix.
27274950	0	25	theme	Cellulose	0:8	arg1	Conformation					86:97	Conformation	86:97	Conformation	86:97	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	25	theme	Cellulose	0:8	arg1	Synthesis					75:83	Synthesis	75:83	Synthesis	75:83	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	25	theme	Cellulose	0:8	arg1	Materials					42:50	Cellulose, Chitosan and Keratin Composite Materials	0:50	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.	0:113	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	7	26	from	KER	1509:1511	arg1	inter-					1473:1478	inter-	1473:1478	inter-	1473:1478	It seems that during dissolution by [BMIm+Cl-], the inter- and intramolecular forces in KER were broken thereby destroying its secondary structure.
27274950	5	27	theme	accurate	1112:1119	arg1	information					1121:1131	more accurate information	1107:1131	more accurate information	1107:1131	A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information.
27274950	9	28	theme	α-helix	1800:1806	arg1	reformation					1781:1791	reformation	1781:1791	reformation of the α-helix	1781:1806	However, in the presence of either CEL or CS, the chains seem to prefer the extended form thereby hindering reformation of the α-helix.
27274950	1	29	theme	[CEL/CS+KER	222:232	arg1	composites					235:244	[CEL/CS+KER] composites	222:244	[CEL/CS+KER] composites formed to have better mechanical strength and wider utilization	222:308	A method was developed in which cellulose (CEL) and/or chitosan (CS) were added to keratin (KER) to enable [CEL/CS+KER] composites formed to have better mechanical strength and wider utilization.
27274950	4	30	theme	bonds	770:774	arg1	bands					755:759	the FTIR bands	746:759	the FTIR bands of amide bonds to determine secondary structure of proteins	746:819	We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting.
27274950	9	31	theme	CS	1715:1716	arg1	presence					1689:1696	the presence	1685:1696	the presence of either CEL or CS	1685:1716	However, in the presence of either CEL or CS, the chains seem to prefer the extended form thereby hindering reformation of the α-helix.
27274950	6	32	from	KER	1401:1403	arg1	wool					1415:1418	native wool	1408:1418	native wool	1408:1418	Results obtained with this method agree well with those by XRD, namely they indicate that although KER retains its second structure when incorporated into the [CEL+CS] composites, it has relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool.
27274950	0	33	theme	Chitosan	11:18	arg1	Conformation					86:97	Conformation	86:97	Conformation	86:97	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	33	theme	Chitosan	11:18	arg1	Synthesis					75:83	Synthesis	75:83	Synthesis	75:83	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	33	theme	Chitosan	11:18	arg1	Materials					42:50	Cellulose, Chitosan and Keratin Composite Materials	0:50	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.	0:113	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	4	34	theme	amide	764:768	arg1	bonds					770:774	amide bonds	764:774	amide bonds	764:774	We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting.
27274950	3	35	theme	XRD	521:523	arg1	results					549:555	FTIR, XRD, 13C CP-MAS NMR and SEM results	515:555	FTIR, XRD, 13C CP-MAS NMR and SEM results	515:555	FTIR, XRD, 13C CP-MAS NMR and SEM results confirm that KER, CS and CEL remain chemically intact and distributed homogeneously in the composites.
27274950	11	36	theme	mechanical	2038:2047	arg1	strength					2049:2056	mechanical strength	2038:2056	mechanical strength	2038:2056	As anticipated, results of tensile strength and TGA confirm that adding CEL or CS into KER substantially increase the mechanical strength and thermal stability of the [CS/CEL+KER] composites.
27274950	10	37	theme	β-sheet	1911:1917	arg1	content					1881:1887	lower content	1875:1887	lower content of α-helix and higher β-sheet	1875:1917	Consequently, the KER in these matrices may adopt structures with lower content of α-helix and higher β-sheet.
27274950	0	38	theme	Composite	32:40	arg1	Conformation					86:97	Conformation	86:97	Conformation	86:97	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	38	theme	Composite	32:40	arg1	Synthesis					75:83	Synthesis	75:83	Synthesis	75:83	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	38	theme	Composite	32:40	arg1	Materials					42:50	Cellulose, Chitosan and Keratin Composite Materials	0:50	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.	0:113	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	2	39	dep	used	444:447	arg1	[BMIm+Cl-					433:441	[BMIm+Cl-]	433:442	[BMIm+Cl-] used (at least 88%)	433:462	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	2	39	dep	used	444:447	arg1	%					461:461	at least 88%	450:461	at least 88%	450:461	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	10	40	theme	α-helix	1892:1898	arg1	content					1881:1887	lower content	1875:1887	lower content of α-helix and higher β-sheet	1875:1917	Consequently, the KER in these matrices may adopt structures with lower content of α-helix and higher β-sheet.
27274950	0	41	theme	Keratin	24:30	arg1	Conformation					86:97	Conformation	86:97	Conformation	86:97	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	41	theme	Keratin	24:30	arg1	Synthesis					75:83	Synthesis	75:83	Synthesis	75:83	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	41	theme	Keratin	24:30	arg1	Materials					42:50	Cellulose, Chitosan and Keratin Composite Materials	0:50	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.	0:113	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	5	42	theme	least	988:992	arg1	squares					994:1000	the partial least squares	976:1000	the partial least squares regression analysis (PLSR) of the amide bands	976:1046	A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information.
27274950	11	43	theme	strength	1955:1962	arg1	results					1936:1942	results	1936:1942	results of tensile strength and TGA	1936:1970	As anticipated, results of tensile strength and TGA confirm that adding CEL or CS into KER substantially increase the mechanical strength and thermal stability of the [CS/CEL+KER] composites.
27274950	3	44	theme	FTIR	515:518	arg1	results					549:555	FTIR, XRD, 13C CP-MAS NMR and SEM results	515:555	FTIR, XRD, 13C CP-MAS NMR and SEM results	515:555	FTIR, XRD, 13C CP-MAS NMR and SEM results confirm that KER, CS and CEL remain chemically intact and distributed homogeneously in the composites.
27274950	0	45	dep	Materials	42:50	arg1	Conformation					86:97	Conformation	86:97	Conformation	86:97	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	45	dep	Materials	42:50	arg1	Synthesis					75:83	Synthesis	75:83	Synthesis	75:83	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	45	dep	Materials	42:50	arg1	Materials					42:50	Cellulose, Chitosan and Keratin Composite Materials	0:50	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.	0:113	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	45	dep	Materials	42:50	arg1	Properties					103:112	Properties	103:112	Properties	103:112	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	2	46	theme	sole	391:394	arg1	solvent					396:402	the sole solvent	387:402	the sole solvent	387:402	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	2	46	theme	sole	391:394	arg1	chloride					335:342	Butylmethylimmidazolium chloride	311:342	Butylmethylimmidazolium chloride ([BMIm+Cl-])	311:355	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	5	47	theme	squares	994:1000	arg1	PLSR					1023:1026	PLSR	1023:1026	PLSR	1023:1026	A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information.
27274950	5	47	theme	squares	994:1000	arg1	analysis					1013:1020	the partial least squares regression analysis	976:1020	the partial least squares regression analysis (PLSR) of the amide bands	976:1046	A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information.
27274950	4	48	theme	FTIR	750:753	arg1	bands					755:759	the FTIR bands	746:759	the FTIR bands of amide bonds to determine secondary structure of proteins	746:819	We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting.
27274950	4	49	dep	bands	755:759	arg1	determine					779:787	determine	779:787	to determine secondary structure of proteins	776:819	We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting.
27274950	5	50	theme	partial	980:986	arg1	squares					994:1000	the partial least squares	976:1000	the partial least squares regression analysis (PLSR) of the amide bands	976:1046	A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information.
27274950	5	51	theme	regression	1002:1011	arg1	PLSR					1023:1026	PLSR	1023:1026	PLSR	1023:1026	A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information.
27274950	5	51	theme	regression	1002:1011	arg1	analysis					1013:1020	the partial least squares regression analysis	976:1020	the partial least squares regression analysis (PLSR) of the amide bands	976:1046	A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information.
27274950	3	52	theme	NMR	537:539	arg1	results					549:555	FTIR, XRD, 13C CP-MAS NMR and SEM results	515:555	FTIR, XRD, 13C CP-MAS NMR and SEM results	515:555	FTIR, XRD, 13C CP-MAS NMR and SEM results confirm that KER, CS and CEL remain chemically intact and distributed homogeneously in the composites.
27274950	4	53	theme	curve	938:942	arg1	fitting					944:950	curve fitting	938:950	curve fitting	938:950	We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting.
27274950	10	54	with	structures	1859:1868	arg1	content					1881:1887	lower content	1875:1887	lower content of α-helix and higher β-sheet	1875:1917	Consequently, the KER in these matrices may adopt structures with lower content of α-helix and higher β-sheet.
27274950	6	55	dep	α-helix	1338:1344	arg1	higher					1347:1352	higher	1347:1352	higher	1347:1352	Results obtained with this method agree well with those by XRD, namely they indicate that although KER retains its second structure when incorporated into the [CEL+CS] composites, it has relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool.
27274950	7	56	dep	inter-	1473:1478	arg1	forces					1499:1504	forces	1499:1504	forces	1499:1504	It seems that during dissolution by [BMIm+Cl-], the inter- and intramolecular forces in KER were broken thereby destroying its secondary structure.
27274950	7	56	dep	inter-	1473:1478	arg1	the					1469:1471	the	1469:1471	the	1469:1471	It seems that during dissolution by [BMIm+Cl-], the inter- and intramolecular forces in KER were broken thereby destroying its secondary structure.
27274950	4	57	theme	bands	755:759	arg1	deconvolution					729:741	the deconvolution	725:741	the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins	725:819	We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting.
27274950	3	58	theme	13C	526:528	arg1	NMR					537:539	13C CP-MAS NMR	526:539	13C CP-MAS NMR	526:539	FTIR, XRD, 13C CP-MAS NMR and SEM results confirm that KER, CS and CEL remain chemically intact and distributed homogeneously in the composites.
27274950	10	59	from	KER	1827:1829	arg1	matrices					1840:1847	these matrices	1834:1847	these matrices	1834:1847	Consequently, the KER in these matrices may adopt structures with lower content of α-helix and higher β-sheet.
27274950	10	60	theme	higher	1904:1909	arg1	β-sheet					1911:1917	higher β-sheet	1904:1917	higher β-sheet	1904:1917	Consequently, the KER in these matrices may adopt structures with lower content of α-helix and higher β-sheet.
27274950	6	61	theme	[CEL+CS	1293:1299	arg1	composites					1302:1311	the [CEL+CS] composites	1289:1311	the [CEL+CS] composites	1289:1311	Results obtained with this method agree well with those by XRD, namely they indicate that although KER retains its second structure when incorporated into the [CEL+CS] composites, it has relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool.
27274950	10	62	theme	lower	1875:1879	arg1	content					1881:1887	lower content	1875:1887	lower content of α-helix and higher β-sheet	1875:1917	Consequently, the KER in these matrices may adopt structures with lower content of α-helix and higher β-sheet.
27274950	3	63	theme	CP-MAS	530:535	arg1	NMR					537:539	13C CP-MAS NMR	526:539	13C CP-MAS NMR	526:539	FTIR, XRD, 13C CP-MAS NMR and SEM results confirm that KER, CS and CEL remain chemically intact and distributed homogeneously in the composites.
27274950	6	64	theme	random	1365:1370	arg1	form					1372:1375	relatively lower α-helix, higher β-turn and random form	1321:1375	relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool	1321:1418	Results obtained with this method agree well with those by XRD, namely they indicate that although KER retains its second structure when incorporated into the [CEL+CS] composites, it has relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool.
27274950	0	65	theme	Facile	53:58	arg1	Synthesis					75:83	Synthesis	75:83	Synthesis	75:83	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	0	65	theme	Facile	53:58	arg1	Materials					42:50	Cellulose, Chitosan and Keratin Composite Materials	0:50	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.	0:113	Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties.
27274950	1	66	theme	mechanical	268:277	arg1	strength					279:286	mechanical strength	268:286	mechanical strength	268:286	A method was developed in which cellulose (CEL) and/or chitosan (CS) were added to keratin (KER) to enable [CEL/CS+KER] composites formed to have better mechanical strength and wider utilization.
27274950	11	67	dep	strength	2049:2056	arg1	the					2034:2036	the	2034:2036	the	2034:2036	As anticipated, results of tensile strength and TGA confirm that adding CEL or CS into KER substantially increase the mechanical strength and thermal stability of the [CS/CEL+KER] composites.
27274950	2	68	theme	Butylmethylimmidazolium	311:333	arg1	liquid					367:372	liquid	367:372	liquid	367:372	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	2	68	theme	Butylmethylimmidazolium	311:333	arg1	solvent					396:402	the sole solvent	387:402	the sole solvent	387:402	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	2	68	theme	Butylmethylimmidazolium	311:333	arg1	[BMIm+Cl-					345:353	[BMIm+Cl-	345:353	[BMIm+Cl-	345:353	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	2	68	theme	Butylmethylimmidazolium	311:333	arg1	chloride					335:342	Butylmethylimmidazolium chloride	311:342	Butylmethylimmidazolium chloride ([BMIm+Cl-])	311:355	Butylmethylimmidazolium chloride ([BMIm+Cl-]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl-] used (at least 88%) was recovered, the method is green and recyclable.
27274950	11	69	theme	composites	2100:2109	arg1	strength					2049:2056	mechanical strength	2038:2056	mechanical strength	2038:2056	As anticipated, results of tensile strength and TGA confirm that adding CEL or CS into KER substantially increase the mechanical strength and thermal stability of the [CS/CEL+KER] composites.
27274950	11	69	theme	composites	2100:2109	arg1	stability					2070:2078	thermal stability	2062:2078	thermal stability	2062:2078	As anticipated, results of tensile strength and TGA confirm that adding CEL or CS into KER substantially increase the mechanical strength and thermal stability of the [CS/CEL+KER] composites.
27274950	9	70	theme	extended	1749:1756	arg1	form					1758:1761	the extended form	1745:1761	the extended form	1745:1761	However, in the presence of either CEL or CS, the chains seem to prefer the extended form thereby hindering reformation of the α-helix.
28275130	9	0	theme	score	1493:1497	arg1	%					1508:1508	0.04 SD score (SDS); 95% CI: 0.00	1485:1517	0.04 SD score (SDS); 95% CI: 0.00	1485:1517	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	7	1	theme	fat-free	992:999	arg1	index					1006:1010	fat-free mass index	992:1010	fat-free mass index	992:1010	At 6 y of age, we further measured fat mass index (FMI) and fat-free mass index with dual-energy X-ray absorptiometry.
28275130	9	2	theme	SDS	1500:1502	arg1	%					1508:1508	0.04 SD score (SDS); 95% CI: 0.00	1485:1517	0.04 SD score (SDS); 95% CI: 0.00	1485:1517	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	1	3	theme	obesity	242:248	arg1	risk					234:237	increased risk	224:237	increased risk of obesity	224:248	Background: High intake of sugar-containing beverages (SCBs) has been linked to increased risk of obesity.
28275130	10	4	theme	children	1760:1767	arg1	SDS					1715:1717	0.04 SDS	1710:1717	a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children	1636:1767	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	4	theme	children	1760:1767	arg1	CI					1724:1725	95% CI	1720:1725	95% CI	1720:1725	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	7	5	theme	mass	971:974	arg1	index					976:980	fat mass index	967:980	fat mass index (FMI)	967:986	At 6 y of age, we further measured fat mass index (FMI) and fat-free mass index with dual-energy X-ray absorptiometry.
28275130	7	5	theme	mass	971:974	arg1	FMI					983:985	FMI	983:985	FMI	983:985	At 6 y of age, we further measured fat mass index (FMI) and fat-free mass index with dual-energy X-ray absorptiometry.
28275130	2	6	theme	intake	280:285	arg1	associations					260:271	associations	260:271	associations of SCB intake during pregnancy with child body composition	260:330	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	1	7	theme	sugar-containing	171:186	arg1	SCBs					199:202	SCBs	199:202	SCBs	199:202	Background: High intake of sugar-containing beverages (SCBs) has been linked to increased risk of obesity.
28275130	1	7	theme	sugar-containing	171:186	arg1	beverages					188:196	sugar-containing beverages	171:196	sugar-containing beverages (SCBs)	171:203	Background: High intake of sugar-containing beverages (SCBs) has been linked to increased risk of obesity.
28275130	9	8	theme	SCB	1112:1114	arg1	intake					1116:1121	SCB intake	1112:1121	SCB intake	1112:1121	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	10	9	theme	total	1555:1559	arg1	SCBs					1561:1564	total SCBs	1555:1564	total SCBs	1555:1564	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	10	theme	fruit	1697:1701	arg1	juice					1703:1707	fruit juice	1697:1707	a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children	1636:1767	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	0	11	theme	Generation	124:133	arg1	Study					137:141	the Generation R Study	120:141	the Generation R Study	120:141	Mothers' intake of sugar-containing beverages during pregnancy and body composition of their children during childhood: the Generation R Study.
28275130	3	12	dep	juice	568:572	arg1	i.e.					556:559	i.e.	556:559	i.e.	556:559	In addition, we examined different types of SCBs (i.e., fruit juice, soda, and concentrate).
28275130	11	13	dep	significant	1798:1808	arg1	<					1813:1813	P < 0.05	1811:1818	P < 0.05	1811:1818	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	7	14	theme	age	942:944	arg1	y					937:937	6 y	935:937	6 y of age	935:944	At 6 y of age, we further measured fat mass index (FMI) and fat-free mass index with dual-energy X-ray absorptiometry.
28275130	1	15	attach	linked	214:219	arg1	risk					234:237	increased risk	224:237	increased risk of obesity	224:248	Background: High intake of sugar-containing beverages (SCBs) has been linked to increased risk of obesity.
28275130	1	15	attach	linked	214:219	arg2	intake					161:166	High intake	156:166	High intake of sugar-containing beverages (SCBs)	156:203	Background: High intake of sugar-containing beverages (SCBs) has been linked to increased risk of obesity.
28275130	3	16	theme	fruit	562:566	arg1	juice					568:572	fruit juice	562:572	fruit juice	562:572	In addition, we examined different types of SCBs (i.e., fruit juice, soda, and concentrate).
28275130	9	17	dep	associated	1420:1429	arg1	%					1508:1508	0.04 SD score (SDS); 95% CI: 0.00	1485:1517	0.04 SD score (SDS); 95% CI: 0.00	1485:1517	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	9	18	theme	total	1388:1392	arg1	intake					1398:1403	mothers' total SCB intake	1379:1403	mothers' total SCB intake	1379:1403	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	11	19	theme	weight	1865:1870	arg1	gain					1872:1875	gestational weight gain	1853:1875	gestational weight gain	1853:1875	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	9	20	theme	body	1160:1163	arg1	composition					1165:1175	body composition	1160:1175	body composition	1160:1175	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	5	21	theme	Energy-adjusted	733:747	arg1	intake					753:758	Energy-adjusted SCB intake	733:758	Energy-adjusted SCB intake	733:758	Energy-adjusted SCB intake was assessed in the first trimester with a food-frequency questionnaire.
28275130	11	22	theme	birth	1878:1882	arg1	weight					1884:1889	birth weight	1878:1889	birth weight	1878:1889	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	11	23	theme	fat	2100:2102	arg1	mass					2104:2107	higher fat mass	2093:2107	higher fat mass	2093:2107	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	2	24	theme	body	488:491	arg1	composition					493:503	body composition	488:503	body composition	488:503	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	4	25	theme	prospective	671:681	arg1	Study					662:666	the Generation R Study	645:666	the Generation R Study	645:666	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	4	25	theme	prospective	671:681	arg1	cohort					683:688	a prospective cohort	669:688	a prospective cohort from fetal life onward in the Netherlands	669:730	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	6	26	theme	age	903:905	arg1	y					898:898	y	898:898	y of age	898:905	Anthropometric data of the children were collected repeatedly ≤6 y of age, and BMI was calculated.
28275130	9	27	theme	linear	1210:1215	arg1	models.Results					1238:1251	multivariable linear mixed and regression models.Results	1196:1251	multivariable linear mixed and regression models.Results	1196:1251	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	0	28	theme	beverages	36:44	arg1	intake					9:14	Mothers' intake	0:14	Mothers' intake of sugar-containing beverages during pregnancy	0:61	Mothers' intake of sugar-containing beverages during pregnancy and body composition of their children during childhood: the Generation R Study.
28275130	0	28	theme	beverages	36:44	arg1	composition					72:82	body composition	67:82	body composition of their children during childhood	67:117	Mothers' intake of sugar-containing beverages during pregnancy and body composition of their children during childhood: the Generation R Study.
28275130	11	29	theme	early	2055:2059	arg1	childhood					2061:2069	early childhood	2055:2069	early childhood	2055:2069	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	4	30	theme	R	660:660	arg1	Study					662:666	the Generation R Study	645:666	the Generation R Study	645:666	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	4	30	theme	R	660:660	arg1	cohort					683:688	a prospective cohort	669:688	a prospective cohort from fetal life onward in the Netherlands	669:730	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	9	31	with	Associations	1096:1107	arg1	trajectories					1143:1154	children's BMI trajectories	1128:1154	children's BMI trajectories	1128:1154	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	9	31	with	Associations	1096:1107	arg1	composition					1165:1175	body composition	1160:1175	body composition	1160:1175	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	9	32	theme	≤6	1451:1452	arg1	y					1454:1454	children's BMI ≤6 y	1436:1454	children's BMI ≤6 y of age [per serving per day	1436:1482	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	4	33	from	onward	706:711	arg1	Study					662:666	the Generation R Study	645:666	the Generation R Study	645:666	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	4	33	from	onward	706:711	arg1	cohort					683:688	a prospective cohort	669:688	a prospective cohort from fetal life onward in the Netherlands	669:730	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	11	34	theme	additional	1827:1836	arg1	adjustment					1838:1847	additional adjustment	1827:1847	additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass	1827:2107	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	11	35	theme	insulin	1907:1913	arg1	concentrations.Conclusion					1915:1939	children's insulin concentrations.Conclusion	1896:1939	children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass	1896:2107	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	0	36	theme	sugar-containing	19:34	arg1	beverages					36:44	sugar-containing beverages	19:44	sugar-containing beverages	19:44	Mothers' intake of sugar-containing beverages during pregnancy and body composition of their children during childhood: the Generation R Study.
28275130	7	37	theme	dual-energy	1017:1027	arg1	absorptiometry					1035:1048	dual-energy X-ray absorptiometry	1017:1048	dual-energy X-ray absorptiometry	1017:1048	At 6 y of age, we further measured fat mass index (FMI) and fat-free mass index with dual-energy X-ray absorptiometry.
28275130	9	38	theme	[per	1463:1466	arg1	y					1454:1454	children's BMI ≤6 y	1436:1454	children's BMI ≤6 y of age [per serving per day	1436:1482	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	2	39	theme	body	441:444	arg1	BMI					458:460	BMI	458:460	BMI	458:460	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	2	39	theme	body	441:444	arg1	index					451:455	children's body mass index	430:455	children's body mass index (BMI)	430:461	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	2	40	theme	body	315:318	arg1	composition					320:330	child body composition	309:330	child body composition	309:330	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	9	41	theme	linear	1267:1272	arg1	models					1280:1285	linear mixed models	1267:1285	linear mixed models	1267:1285	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	11	42	theme	maternal	1966:1973	arg1	intake					1979:1984	maternal SCB intake	1966:1984	maternal SCB intake during pregnancy	1966:2001	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	4	43	theme	life	701:704	arg1	onward					706:711	fetal life onward	695:711	fetal life onward	695:711	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	9	44	theme	SD	1490:1491	arg1	%					1508:1508	0.04 SD score (SDS); 95% CI: 0.00	1485:1517	0.04 SD score (SDS); 95% CI: 0.00	1485:1517	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	10	45	dep	FMI	1645:1647	arg1	SDS					1715:1717	0.04 SDS	1710:1717	a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children	1636:1767	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	45	dep	FMI	1645:1647	arg1	CI					1676:1677	95% CI	1672:1677	95% CI	1672:1677	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	45	dep	FMI	1645:1647	arg1	SCBs					1656:1659	[total SCBs	1649:1659	a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children	1636:1767	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	45	dep	FMI	1645:1647	arg1	SDS					1667:1669	0.05 SDS	1662:1669	a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children	1636:1767	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	45	dep	FMI	1645:1647	arg1	CI					1724:1725	95% CI	1720:1725	95% CI	1720:1725	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	45	dep	FMI	1645:1647	arg1	juice					1703:1707	fruit juice	1697:1707	a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children	1636:1767	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	1	46	theme	increased	224:232	arg1	risk					234:237	increased risk	224:237	increased risk of obesity	224:248	Background: High intake of sugar-containing beverages (SCBs) has been linked to increased risk of obesity.
28275130	6	47	theme	Anthropometric	833:846	arg1	data					848:851	Anthropometric data	833:851	Anthropometric data of the children	833:867	Anthropometric data of the children were collected repeatedly ≤6 y of age, and BMI was calculated.
28275130	10	48	theme	%	1674:1674	arg1	SDS					1667:1669	0.05 SDS	1662:1669	a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children	1636:1767	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	48	theme	%	1674:1674	arg1	CI					1676:1677	95% CI	1672:1677	95% CI	1672:1677	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	5	49	theme	first	780:784	arg1	trimester					786:794	the first trimester	776:794	the first trimester	776:794	Energy-adjusted SCB intake was assessed in the first trimester with a food-frequency questionnaire.
28275130	1	50	theme	High	156:159	arg1	intake					161:166	High intake	156:166	High intake of sugar-containing beverages (SCBs)	156:203	Background: High intake of sugar-containing beverages (SCBs) has been linked to increased risk of obesity.
28275130	10	51	theme	6-y-old	1752:1758	arg1	children					1760:1767	the 6-y-old children	1748:1767	the 6-y-old children	1748:1767	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	7	52	theme	fat	967:969	arg1	index					976:980	fat mass index	967:980	fat mass index (FMI)	967:986	At 6 y of age, we further measured fat mass index (FMI) and fat-free mass index with dual-energy X-ray absorptiometry.
28275130	7	52	theme	fat	967:969	arg1	FMI					983:985	FMI	983:985	FMI	983:985	At 6 y of age, we further measured fat mass index (FMI) and fat-free mass index with dual-energy X-ray absorptiometry.
28275130	10	53	theme	fruit	1570:1574	arg1	juice					1576:1580	fruit juice	1570:1580	fruit juice	1570:1580	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	9	54	theme	SCB	1347:1349	arg1	intake					1351:1356	the SCB intake	1343:1356	the SCB intake of the child itself	1343:1376	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	2	55	theme	SCB	276:278	arg1	intake					280:285	SCB intake	276:285	SCB intake during pregnancy with child body composition	276:330	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	5	56	theme	SCB	749:751	arg1	intake					753:758	Energy-adjusted SCB intake	733:758	Energy-adjusted SCB intake	733:758	Energy-adjusted SCB intake was assessed in the first trimester with a food-frequency questionnaire.
28275130	3	57	theme	different	531:539	arg1	types					541:545	different types	531:545	different types of SCBs (i.e., fruit juice, soda, and concentrate)	531:596	In addition, we examined different types of SCBs (i.e., fruit juice, soda, and concentrate).
28275130	0	58	theme	R	135:135	arg1	Study					137:141	the Generation R Study	120:141	the Generation R Study	120:141	Mothers' intake of sugar-containing beverages during pregnancy and body composition of their children during childhood: the Generation R Study.
28275130	9	59	theme	intake	1116:1121	arg1	Associations					1096:1107	Associations	1096:1107	Associations of SCB intake with children's BMI trajectories and body composition	1096:1175	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	3	60	theme	SCBs	550:553	arg1	types					541:545	different types	531:545	different types of SCBs (i.e., fruit juice, soda, and concentrate)	531:596	In addition, we examined different types of SCBs (i.e., fruit juice, soda, and concentrate).
28275130	0	61	dep	Study	137:141	arg1	intake					9:14	Mothers' intake	0:14	Mothers' intake of sugar-containing beverages during pregnancy	0:61	Mothers' intake of sugar-containing beverages during pregnancy and body composition of their children during childhood: the Generation R Study.
28275130	0	61	dep	Study	137:141	arg1	composition					72:82	body composition	67:82	body composition of their children during childhood	67:117	Mothers' intake of sugar-containing beverages during pregnancy and body composition of their children during childhood: the Generation R Study.
28275130	9	62	theme	child	1365:1369	arg1	intake					1351:1356	the SCB intake	1343:1356	the SCB intake of the child itself	1343:1376	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	2	63	theme	composition	493:503	arg1	measures					476:483	detailed measures	467:483	detailed measures of body composition	467:503	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	2	63	theme	composition	493:503	arg1	BMI					458:460	BMI	458:460	BMI	458:460	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	2	63	theme	composition	493:503	arg1	index					451:455	children's body mass index	430:455	children's body mass index (BMI)	430:461	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	10	64	dep	CI	1724:1725	arg1	0.01					1728:1731	0.01	1728:1731	0.01	1728:1731	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	64	dep	CI	1724:1725	arg1	SDS					1739:1741	0.06 SDS	1734:1741	0.06 SDS	1734:1741	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	6	65	theme	children	860:867	arg1	data					848:851	Anthropometric data	833:851	Anthropometric data of the children	833:867	Anthropometric data of the children were collected repeatedly ≤6 y of age, and BMI was calculated.
28275130	4	66	theme	mother-child	623:634	arg1	pairs					636:640	3312 mother-child pairs	618:640	3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands	618:730	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	9	67	theme	BMI	1139:1141	arg1	trajectories					1143:1154	children's BMI trajectories	1128:1154	children's BMI trajectories	1128:1154	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	10	68	theme	concentrate	1602:1612	arg1	intakes					1544:1550	intakes	1544:1550	intakes of total SCBs and fruit juice, but not of soda or concentrate,	1544:1613	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	69	theme	SCBs	1561:1564	arg1	intakes					1544:1550	intakes	1544:1550	intakes of total SCBs and fruit juice, but not of soda or concentrate,	1544:1613	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	0	70	theme	body	67:70	arg1	composition					72:82	body composition	67:82	body composition of their children during childhood	67:117	Mothers' intake of sugar-containing beverages during pregnancy and body composition of their children during childhood: the Generation R Study.
28275130	10	71	theme	soda	1594:1597	arg1	intakes					1544:1550	intakes	1544:1550	intakes of total SCBs and fruit juice, but not of soda or concentrate,	1544:1613	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	1	72	theme	beverages	188:196	arg1	intake					161:166	High intake	156:166	High intake of sugar-containing beverages (SCBs)	156:203	Background: High intake of sugar-containing beverages (SCBs) has been linked to increased risk of obesity.
28275130	9	73	theme	SCB	1394:1396	arg1	intake					1398:1403	mothers' total SCB intake	1379:1403	mothers' total SCB intake	1379:1403	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	11	74	theme	higher	2093:2098	arg1	mass					2104:2107	higher fat mass	2093:2107	higher fat mass	2093:2107	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	5	75	theme	food-frequency	803:816	arg1	questionnaire					818:830	a food-frequency questionnaire	801:830	a food-frequency questionnaire	801:830	Energy-adjusted SCB intake was assessed in the first trimester with a food-frequency questionnaire.
28275130	11	76	theme	gestational	1853:1863	arg1	gain					1872:1875	gestational weight gain	1853:1875	gestational weight gain	1853:1875	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	10	77	theme	%	1722:1722	arg1	SDS					1715:1717	0.04 SDS	1710:1717	a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children	1636:1767	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	77	theme	%	1722:1722	arg1	CI					1724:1725	95% CI	1720:1725	95% CI	1720:1725	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	0	78	theme	children	93:100	arg1	intake					9:14	Mothers' intake	0:14	Mothers' intake of sugar-containing beverages during pregnancy	0:61	Mothers' intake of sugar-containing beverages during pregnancy and body composition of their children during childhood: the Generation R Study.
28275130	0	78	theme	children	93:100	arg1	composition					72:82	body composition	67:82	body composition of their children during childhood	67:117	Mothers' intake of sugar-containing beverages during pregnancy and body composition of their children during childhood: the Generation R Study.
28275130	4	79	from	cohort	683:688	arg1	Netherlands					720:730	the Netherlands	716:730	the Netherlands	716:730	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	9	80	theme	multivariable	1196:1208	arg1	models.Results					1238:1251	multivariable linear mixed and regression models.Results	1196:1251	multivariable linear mixed and regression models.Results	1196:1251	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	2	81	theme	detailed	467:474	arg1	measures					476:483	detailed measures	467:483	detailed measures of body composition	467:503	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	9	82	theme	mixed	1217:1221	arg1	models.Results					1238:1251	multivariable linear mixed and regression models.Results	1196:1251	multivariable linear mixed and regression models.Results	1196:1251	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	9	83	theme	BMI	1447:1449	arg1	y					1454:1454	children's BMI ≤6 y	1436:1454	children's BMI ≤6 y of age [per serving per day	1436:1482	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	4	84	theme	Generation	649:658	arg1	Study					662:666	the Generation R Study	645:666	the Generation R Study	645:666	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	4	84	theme	Generation	649:658	arg1	cohort					683:688	a prospective cohort	669:688	a prospective cohort from fetal life onward in the Netherlands	669:730	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	7	85	theme	X-ray	1029:1033	arg1	absorptiometry					1035:1048	dual-energy X-ray absorptiometry	1017:1048	dual-energy X-ray absorptiometry	1017:1048	At 6 y of age, we further measured fat mass index (FMI) and fat-free mass index with dual-energy X-ray absorptiometry.
28275130	9	86	theme	regression	1227:1236	arg1	models.Results					1238:1251	multivariable linear mixed and regression models.Results	1196:1251	multivariable linear mixed and regression models.Results	1196:1251	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	11	87	theme	P	1811:1811	arg1	<					1813:1813	P < 0.05	1811:1818	P < 0.05	1811:1818	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	10	88	dep	CI	1676:1677	arg1	0.01					1680:1683	0.01	1680:1683	0.01	1680:1683	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	10	88	dep	CI	1676:1677	arg1	SDS					1691:1693	0.08 SDS	1686:1693	0.08 SDS	1686:1693	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	4	89	theme	Study	662:666	arg1	pairs					636:640	3312 mother-child pairs	618:640	3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands	618:730	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	9	90	dep	%	1508:1508	arg1	SDS					1525:1527	0.07 SDS	1520:1527	0.07 SDS	1520:1527	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	9	91	theme	age	1459:1461	arg1	[per					1463:1466	age [per	1459:1466	age [per serving per day	1459:1482	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	2	92	theme	mass	446:449	arg1	BMI					458:460	BMI	458:460	BMI	458:460	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	2	92	theme	mass	446:449	arg1	index					451:455	children's body mass index	430:455	children's body mass index (BMI)	430:461	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	2	93	theme	SCB	382:384	arg1	intake					386:391	SCB intake	382:391	SCB intake during pregnancy	382:408	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	7	94	theme	mass	1001:1004	arg1	index					1006:1010	fat-free mass index	992:1010	fat-free mass index	992:1010	At 6 y of age, we further measured fat mass index (FMI) and fat-free mass index with dual-energy X-ray absorptiometry.
28275130	10	95	theme	[total	1649:1654	arg1	SCBs					1656:1659	[total SCBs	1649:1659	a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children	1636:1767	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	2	96	theme	child	309:313	arg1	composition					320:330	child body composition	309:330	child body composition	309:330	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
28275130	9	97	theme	mixed	1274:1278	arg1	models					1280:1285	linear mixed models	1267:1285	linear mixed models	1267:1285	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	9	98	from	models	1280:1285	arg1	Results					1254:1260	Results	1254:1260	Results from linear mixed models	1254:1285	Associations of SCB intake with children's BMI trajectories and body composition were analyzed with multivariable linear mixed and regression models.Results: Results from linear mixed models showed that, after adjustment for confounders including the SCB intake of the child itself, mothers' total SCB intake was positively associated with children's BMI ≤6 y of age [per serving per day: 0.04 SD score (SDS); 95% CI: 0.00, 0.07 SDS].
28275130	11	99	theme	SCB	1975:1977	arg1	intake					1979:1984	maternal SCB intake	1966:1984	maternal SCB intake during pregnancy	1966:2001	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	10	100	theme	higher	1638:1643	arg1	FMI					1645:1647	a higher FMI	1636:1647	a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children	1636:1767	In addition, intakes of total SCBs and fruit juice, but not of soda or concentrate, were associated with a higher FMI [total SCBs: 0.05 SDS (95% CI: 0.01, 0.08 SDS); fruit juice: 0.04 SDS (95% CI: 0.01, 0.06 SDS)] of the 6-y-old children.
28275130	4	101	theme	fetal	695:699	arg1	onward					706:711	fetal life onward	695:711	fetal life onward	695:711	Design: We included 3312 mother-child pairs of the Generation R Study, a prospective cohort from fetal life onward in the Netherlands.
28275130	11	102	dep	concentrations.Conclusion	1915:1939	arg1	suggests					1952:1959	suggests	1952:1959	suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass	1952:2107	These associations remained significant (P < 0.05) after additional adjustment for gestational weight gain, birth weight, and children's insulin concentrations.Conclusion: Our study suggests that maternal SCB intake during pregnancy is positively associated with children's BMI during early childhood and particularly with higher fat mass.
28275130	2	103	with	intake	280:285	arg1	composition					320:330	child body composition	309:330	child body composition	309:330	However, associations of SCB intake during pregnancy with child body composition have been unclear.Objectives: We explored whether SCB intake during pregnancy was associated with children's body mass index (BMI) and detailed measures of body composition.
26012895	5	0	theme	cell-mimicking	1042:1055	arg1	compartments					1057:1068	cell-mimicking compartments	1042:1068	cell-mimicking compartments	1042:1068	In addition, the ability to encapsulate different materials into coacervate droplets using a microfluidic channel structure allows for their use as cell-mimicking compartments.
26012895	3	1	theme	information	765:775	arg1	exchange					745:752	detectable exchange	734:752	detectable exchange of genetic information	734:775	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	3	2	theme	distinct	572:579	arg1	populations					581:591	two distinct populations	568:591	two distinct populations of coacervate droplets containing different DNA oligonucleotides	568:656	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	2	3	theme	narrower	326:333	arg1	distributions					340:352	narrower size distributions	326:352	narrower size distributions	326:352	The formed droplets exhibit improved stability and narrower size distributions for both coacervate compositions when compared to the conventional vortex dispersion techniques.
26012895	4	4	theme	droplet	829:835	arg1	stability					837:845	droplet stability	829:845	droplet stability	829:845	Our results show that the observed improvements in droplet stability and size distribution may be scaled with ease.
26012895	3	5	theme	different	627:635	arg1	oligonucleotides					641:656	different DNA oligonucleotides	627:656	different DNA oligonucleotides	627:656	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	5	6	theme	different	934:942	arg1	materials					944:952	different materials	934:952	different materials	934:952	In addition, the ability to encapsulate different materials into coacervate droplets using a microfluidic channel structure allows for their use as cell-mimicking compartments.
26012895	5	7	theme	channel	1000:1006	arg1	structure					1008:1016	a microfluidic channel structure	985:1016	a microfluidic channel structure	985:1016	In addition, the ability to encapsulate different materials into coacervate droplets using a microfluidic channel structure allows for their use as cell-mimicking compartments.
26012895	3	8	theme	simultaneous	526:537	arg1	formation					539:547	simultaneous formation	526:547	simultaneous formation	526:547	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	3	9	theme	coacervate	596:605	arg1	droplets					607:614	coacervate droplets	596:614	coacervate droplets containing different DNA oligonucleotides	596:656	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	3	10	theme	parallel	486:493	arg1	channels					509:516	two parallel flow-focusing channels	482:516	two parallel flow-focusing channels	482:516	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	5	11	theme	microfluidic	987:998	arg1	structure					1008:1016	a microfluidic channel structure	985:1016	a microfluidic channel structure	985:1016	In addition, the ability to encapsulate different materials into coacervate droplets using a microfluidic channel structure allows for their use as cell-mimicking compartments.
26012895	1	12	with	poly	133:136	arg1	carboxymethyl-dextran					210:230	carboxymethyl-dextran	210:230	carboxymethyl-dextran	210:230	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	1	12	with	poly	133:136	arg1	triphosphate					194:205	adenosine triphosphate	184:205	adenosine triphosphate	184:205	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	2	13	theme	improved	303:310	arg1	stability					312:320	improved stability	303:320	improved stability	303:320	The formed droplets exhibit improved stability and narrower size distributions for both coacervate compositions when compared to the conventional vortex dispersion techniques.
26012895	3	14	theme	flow-focusing	495:507	arg1	channels					509:516	two parallel flow-focusing channels	482:516	two parallel flow-focusing channels	482:516	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	1	15	from	poly	133:136	arg1	formation					95:103	the formation	91:103	the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran	91:230	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	1	15	from	poly	133:136	arg1	droplets					119:126	coacervate droplets	108:126	coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran	108:230	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	1	16	theme	adenosine	184:192	arg1	triphosphate					194:205	adenosine triphosphate	184:205	adenosine triphosphate	184:205	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	0	17	theme	Microfluidic	0:11	arg1	Formation					13:21	Microfluidic Formation	0:21	Microfluidic Formation of Membrane-Free Aqueous Coacervate	0:57	Microfluidic Formation of Membrane-Free Aqueous Coacervate Droplets in Water.
26012895	4	18	theme	size	851:854	arg1	distribution					856:867	size distribution	851:867	size distribution	851:867	Our results show that the observed improvements in droplet stability and size distribution may be scaled with ease.
26012895	2	19	theme	dispersion	428:437	arg1	techniques					439:448	the conventional vortex dispersion techniques	404:448	the conventional vortex dispersion techniques	404:448	The formed droplets exhibit improved stability and narrower size distributions for both coacervate compositions when compared to the conventional vortex dispersion techniques.
26012895	0	20	theme	Membrane-Free	26:38	arg1	Coacervate					48:57	Membrane-Free Aqueous Coacervate	26:57	Membrane-Free Aqueous Coacervate	26:57	Microfluidic Formation of Membrane-Free Aqueous Coacervate Droplets in Water.
26012895	4	21	from	improvements	813:824	arg1	stability					837:845	droplet stability	829:845	droplet stability	829:845	Our results show that the observed improvements in droplet stability and size distribution may be scaled with ease.
26012895	4	21	from	improvements	813:824	arg1	distribution					856:867	size distribution	851:867	size distribution	851:867	Our results show that the observed improvements in droplet stability and size distribution may be scaled with ease.
26012895	3	22	theme	DNA	637:639	arg1	oligonucleotides					641:656	different DNA oligonucleotides	627:656	different DNA oligonucleotides	627:656	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	2	23	theme	size	335:338	arg1	distributions					340:352	narrower size distributions	326:352	narrower size distributions	326:352	The formed droplets exhibit improved stability and narrower size distributions for both coacervate compositions when compared to the conventional vortex dispersion techniques.
26012895	3	24	theme	populations	581:591	arg1	co-location					553:563	co-location	553:563	co-location	553:563	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	3	24	theme	populations	581:591	arg1	formation					539:547	simultaneous formation	526:547	simultaneous formation	526:547	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	0	25	theme	Coacervate	48:57	arg1	Formation					13:21	Microfluidic Formation	0:21	Microfluidic Formation of Membrane-Free Aqueous Coacervate	0:57	Microfluidic Formation of Membrane-Free Aqueous Coacervate Droplets in Water.
26012895	5	26	theme	coacervate	959:968	arg1	droplets					970:977	coacervate droplets	959:977	coacervate droplets using a microfluidic channel structure	959:1016	In addition, the ability to encapsulate different materials into coacervate droplets using a microfluidic channel structure allows for their use as cell-mimicking compartments.
26012895	3	27	theme	channels	509:516	arg1	use					475:477	the use	471:477	the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides	471:656	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	0	28	theme	Aqueous	40:46	arg1	Coacervate					48:57	Membrane-Free Aqueous Coacervate	26:57	Membrane-Free Aqueous Coacervate	26:57	Microfluidic Formation of Membrane-Free Aqueous Coacervate Droplets in Water.
26012895	1	29	theme	coacervate	108:117	arg1	droplets					119:126	coacervate droplets	108:126	coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran	108:230	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	1	30	theme	diallyldimethylammonium	138:160	arg1	poly					133:136	poly	133:136	poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran	133:230	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	1	30	theme	diallyldimethylammonium	138:160	arg1	chloride					162:169	diallyldimethylammonium chloride	138:169	diallyldimethylammonium chloride	138:169	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	1	31	theme	droplets	119:126	arg1	formation					95:103	the formation	91:103	the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran	91:230	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	1	32	from	formation	95:103	arg1	poly					133:136	poly	133:136	poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran	133:230	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	1	32	from	formation	95:103	arg1	chloride					162:169	diallyldimethylammonium chloride	138:169	diallyldimethylammonium chloride	138:169	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	3	33	contain	containing	616:625	arg2	oligonucleotides					641:656	different DNA oligonucleotides	627:656	different DNA oligonucleotides	627:656	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	3	33	contain	containing	616:625	arg1	droplets					607:614	coacervate droplets	596:614	coacervate droplets containing different DNA oligonucleotides	596:656	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	3	34	theme	genetic	757:763	arg1	information					765:775	genetic information	757:775	genetic information	757:775	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	3	35	theme	close	699:703	arg1	proximity					705:713	close proximity	699:713	close proximity up to 48 h	699:724	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	4	36	theme	observed	804:811	arg1	improvements					813:824	the observed improvements	800:824	the observed improvements in droplet stability and size distribution	800:867	Our results show that the observed improvements in droplet stability and size distribution may be scaled with ease.
26012895	1	37	theme	microfluidic	240:251	arg1	system					267:272	a microfluidic flow-focusing system	238:272	a microfluidic flow-focusing system	238:272	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	3	38	theme	droplets	607:614	arg1	populations					581:591	two distinct populations	568:591	two distinct populations of coacervate droplets containing different DNA oligonucleotides	568:656	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	3	39	theme	detectable	734:743	arg1	exchange					745:752	detectable exchange	734:752	detectable exchange of genetic information	734:775	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	3	40	dep	formation	539:547	arg1	the					522:524	the	522:524	the	522:524	We also demonstrate the use of two parallel flow-focusing channels for the simultaneous formation and co-location of two distinct populations of coacervate droplets containing different DNA oligonucleotides, and that the populations can coexist in close proximity up to 48 h without detectable exchange of genetic information.
26012895	2	41	theme	coacervate	363:372	arg1	compositions					374:385	both coacervate compositions	358:385	both coacervate compositions	358:385	The formed droplets exhibit improved stability and narrower size distributions for both coacervate compositions when compared to the conventional vortex dispersion techniques.
26012895	1	42	theme	flow-focusing	253:265	arg1	system					267:272	a microfluidic flow-focusing system	238:272	a microfluidic flow-focusing system	238:272	We report on the formation of coacervate droplets from poly(diallyldimethylammonium chloride) with either adenosine triphosphate or carboxymethyl-dextran using a microfluidic flow-focusing system.
26012895	2	43	theme	formed	279:284	arg1	droplets					286:293	The formed droplets	275:293	The formed droplets	275:293	The formed droplets exhibit improved stability and narrower size distributions for both coacervate compositions when compared to the conventional vortex dispersion techniques.
26012895	2	44	theme	vortex	421:426	arg1	techniques					439:448	the conventional vortex dispersion techniques	404:448	the conventional vortex dispersion techniques	404:448	The formed droplets exhibit improved stability and narrower size distributions for both coacervate compositions when compared to the conventional vortex dispersion techniques.
26012895	2	45	theme	conventional	408:419	arg1	techniques					439:448	the conventional vortex dispersion techniques	404:448	the conventional vortex dispersion techniques	404:448	The formed droplets exhibit improved stability and narrower size distributions for both coacervate compositions when compared to the conventional vortex dispersion techniques.
25047626	6	0	theme	CD44	1240:1243	arg1	expression					1226:1235	expression	1226:1235	expression	1226:1235	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	6	0	theme	CD44	1240:1243	arg1	layer					1216:1220	the dorsal layer	1205:1220	the dorsal layer	1205:1220	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	4	1	theme	vascular	856:863	arg1	membrane					874:881	vascular basement membrane	856:881	vascular basement membrane	856:881	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	4	2	from	system	755:760	arg1	sandwiched					787:796	sandwiched	787:796	sandwiched	787:796	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	2	3	theme	rapid	334:338	arg1	mode					340:343	The most rapid mode	325:343	The most rapid mode of tissue infiltration	325:366	The most rapid mode of tissue infiltration occurs along blood vessels or white matter tracts, which represent topological interfaces thought to serve as "tracks" that speed cell migration.
25047626	0	4	theme	migration	63:71	arg1	dissection					38:47	the dissection	34:47	the dissection of tumor cell migration at tissue interfaces	34:92	A composite hydrogel platform for the dissection of tumor cell migration at tissue interfaces.
25047626	5	5	theme	complexes	1034:1042	arg1	formation					1012:1020	formation	1012:1020	formation of adhesive complexes	1012:1042	We find that inclusion of the dorsal HA surface induces formation of adhesive complexes and significantly slows cell migration relative to a free fibronectin-coated surface.
25047626	1	6	theme	prevalent	135:143	arg1	cancer					159:164	the most prevalent primary brain cancer	126:164	the most prevalent primary brain cancer	126:164	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	1	6	theme	prevalent	135:143	arg1	multiforme					108:117	Glioblastoma multiforme	95:117	Glioblastoma multiforme (GBM)	95:123	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	1	7	theme	brain	228:232	arg1	tissue					234:239	brain tissue	228:239	brain tissue	228:239	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	3	8	theme	mechanisms	669:678	arg1	dissection					655:664	reductionist dissection	642:664	reductionist dissection of mechanisms of this interfacial motility	642:707	Despite this observation, the field lacks experimental paradigms that capture key features of these tissue interfaces and allow reductionist dissection of mechanisms of this interfacial motility.
25047626	6	9	theme	dorsal	1266:1271	arg1	surface					1273:1279	the dorsal surface	1262:1279	the dorsal surface	1262:1279	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	6	10	theme	dorsal	1209:1214	arg1	layer					1216:1220	the dorsal layer	1205:1220	the dorsal layer	1205:1220	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	7	11	theme	migration	1408:1416	arg1	speed					1418:1422	migration speed	1408:1422	migration speed	1408:1422	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	0	12	theme	tissue	76:81	arg1	interfaces					83:92	tissue interfaces	76:92	tissue interfaces	76:92	A composite hydrogel platform for the dissection of tumor cell migration at tissue interfaces.
25047626	7	13	theme	myosin	1467:1472	arg1	activation					1474:1483	myosin activation	1467:1483	myosin activation	1467:1483	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	4	14	theme	basement	865:872	arg1	membrane					874:881	vascular basement membrane	856:881	vascular basement membrane	856:881	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	6	15	theme	integrin	1176:1183	arg1	peptides					1193:1200	integrin binding peptides	1176:1200	integrin binding peptides	1176:1200	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	7	16	theme	ventral	1510:1516	arg1	layer					1518:1522	the ventral layer	1506:1522	the ventral layer	1506:1522	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	4	17	theme	fibronectin-coated	808:825	arg1	surface					835:841	a fibronectin-coated ventral surface	806:841	a fibronectin-coated ventral surface representing vascular basement membrane	806:881	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	4	18	theme	culture	747:753	arg1	system					755:760	a culture system	745:760	a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma	745:953	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	6	19	from	inclusion	1163:1171	arg1	expression					1226:1235	expression	1226:1235	expression	1226:1235	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	6	19	from	inclusion	1163:1171	arg1	layer					1216:1220	the dorsal layer	1205:1220	the dorsal layer	1205:1220	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	1	20	theme	primary	145:151	arg1	cancer					159:164	the most prevalent primary brain cancer	126:164	the most prevalent primary brain cancer	126:164	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	1	20	theme	primary	145:151	arg1	multiforme					108:117	Glioblastoma multiforme	95:117	Glioblastoma multiforme (GBM)	95:123	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	2	21	theme	tracks	479:484	arg1	"					485:485	"tracks"	478:485	"tracks" that speed cell migration	478:511	The most rapid mode of tissue infiltration occurs along blood vessels or white matter tracts, which represent topological interfaces thought to serve as "tracks" that speed cell migration.
25047626	3	22	theme	interfacial	688:698	arg1	motility					700:707	this interfacial motility	683:707	this interfacial motility	683:707	Despite this observation, the field lacks experimental paradigms that capture key features of these tissue interfaces and allow reductionist dissection of mechanisms of this interfacial motility.
25047626	5	23	theme	fibronectin-coated	1102:1119	arg1	surface					1121:1127	a free fibronectin-coated surface	1095:1127	a free fibronectin-coated surface	1095:1127	We find that inclusion of the dorsal HA surface induces formation of adhesive complexes and significantly slows cell migration relative to a free fibronectin-coated surface.
25047626	1	24	theme	brain	153:157	arg1	cancer					159:164	the most prevalent primary brain cancer	126:164	the most prevalent primary brain cancer	126:164	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	1	24	theme	brain	153:157	arg1	multiforme					108:117	Glioblastoma multiforme	95:117	Glioblastoma multiforme (GBM)	95:123	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	0	25	theme	composite	2:10	arg1	platform					21:28	A composite hydrogel platform	0:28	A composite hydrogel platform for the dissection of tumor cell migration at tissue interfaces	0:92	A composite hydrogel platform for the dissection of tumor cell migration at tissue interfaces.
25047626	3	26	theme	motility	700:707	arg1	mechanisms					669:678	mechanisms	669:678	mechanisms of this interfacial motility	669:707	Despite this observation, the field lacks experimental paradigms that capture key features of these tissue interfaces and allow reductionist dissection of mechanisms of this interfacial motility.
25047626	2	27	theme	infiltration	355:366	arg1	mode					340:343	The most rapid mode	325:343	The most rapid mode of tissue infiltration	325:366	The most rapid mode of tissue infiltration occurs along blood vessels or white matter tracts, which represent topological interfaces thought to serve as "tracks" that speed cell migration.
25047626	7	28	theme	dorsal	1440:1445	arg1	adhesions					1447:1455	dorsal adhesions	1440:1455	dorsal adhesions	1440:1455	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	4	29	theme	ventral	827:833	arg1	surface					835:841	a fibronectin-coated ventral surface	806:841	a fibronectin-coated ventral surface representing vascular basement membrane	806:881	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	7	30	theme	layer	1518:1522	arg1	activation					1474:1483	myosin activation	1467:1483	myosin activation	1467:1483	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	7	30	theme	layer	1518:1522	arg1	stiffness					1493:1501	the stiffness	1489:1501	the stiffness of the ventral layer	1489:1522	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	2	31	theme	tissue	348:353	arg1	infiltration					355:366	tissue infiltration	348:366	tissue infiltration	348:366	The most rapid mode of tissue infiltration occurs along blood vessels or white matter tracts, which represent topological interfaces thought to serve as "tracks" that speed cell migration.
25047626	6	32	theme	simple	1351:1356	arg1	hindrance					1365:1373	simple steric hindrance	1351:1373	biochemically specific mechanisms rather than simple steric hindrance	1305:1373	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	6	33	theme	steric	1358:1363	arg1	hindrance					1365:1373	simple steric hindrance	1351:1373	biochemically specific mechanisms rather than simple steric hindrance	1305:1373	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	5	34	theme	HA	993:994	arg1	surface					996:1002	the dorsal HA surface	982:1002	the dorsal HA surface	982:1002	We find that inclusion of the dorsal HA surface induces formation of adhesive complexes and significantly slows cell migration relative to a free fibronectin-coated surface.
25047626	2	35	theme	white	398:402	arg1	tracts					411:416	white matter tracts	398:416	white matter tracts	398:416	The most rapid mode of tissue infiltration occurs along blood vessels or white matter tracts, which represent topological interfaces thought to serve as "tracks" that speed cell migration.
25047626	2	36	theme	matter	404:409	arg1	tracts					411:416	white matter tracts	398:416	white matter tracts	398:416	The most rapid mode of tissue infiltration occurs along blood vessels or white matter tracts, which represent topological interfaces thought to serve as "tracks" that speed cell migration.
25047626	7	37	theme	ventral	1580:1586	arg1	layer					1588:1592	the ventral layer	1576:1592	the ventral layer	1576:1592	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	4	38	theme	acid	907:910	arg1	surface					917:923	a dorsal hyaluronic acid (HA) surface	887:923	a dorsal hyaluronic acid (HA) surface	887:923	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	3	39	theme	tissue	614:619	arg1	interfaces					621:630	these tissue interfaces	608:630	these tissue interfaces	608:630	Despite this observation, the field lacks experimental paradigms that capture key features of these tissue interfaces and allow reductionist dissection of mechanisms of this interfacial motility.
25047626	1	40	theme	Glioblastoma	95:106	arg1	GBM					120:122	GBM	120:122	GBM	120:122	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	1	40	theme	Glioblastoma	95:106	arg1	cancer					159:164	the most prevalent primary brain cancer	126:164	the most prevalent primary brain cancer	126:164	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	1	40	theme	Glioblastoma	95:106	arg1	multiforme					108:117	Glioblastoma multiforme	95:117	Glioblastoma multiforme (GBM)	95:123	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	1	41	theme	surgical	269:276	arg1	resection					278:286	surgical resection	269:286	surgical resection	269:286	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	7	42	theme	mechanochemical	1539:1553	arg1	feedback					1555:1562	mechanochemical feedback	1539:1562	mechanochemical feedback directed by the ventral layer	1539:1592	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	3	43	theme	interfaces	621:630	arg1	features					596:603	key features	592:603	key features of these tissue interfaces	592:630	Despite this observation, the field lacks experimental paradigms that capture key features of these tissue interfaces and allow reductionist dissection of mechanisms of this interfacial motility.
25047626	3	44	theme	experimental	556:567	arg1	paradigms					569:577	experimental paradigms	556:577	experimental paradigms that capture key features of these tissue interfaces and allow reductionist dissection of mechanisms of this interfacial motility	556:707	Despite this observation, the field lacks experimental paradigms that capture key features of these tissue interfaces and allow reductionist dissection of mechanisms of this interfacial motility.
25047626	6	45	theme	peptides	1193:1200	arg1	inclusion					1163:1171	inclusion	1163:1171	inclusion of integrin binding peptides in the dorsal layer and expression of CD44	1163:1243	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	7	46	theme	adhesions	1447:1455	arg1	assembly					1428:1435	assembly	1428:1435	assembly of dorsal adhesions	1428:1455	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	7	46	theme	adhesions	1447:1455	arg1	reduction					1395:1403	the reduction	1391:1403	the reduction in migration speed	1391:1422	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	2	47	theme	cell	498:501	arg1	migration					503:511	cell migration	498:511	cell migration	498:511	The most rapid mode of tissue infiltration occurs along blood vessels or white matter tracts, which represent topological interfaces thought to serve as "tracks" that speed cell migration.
25047626	5	48	theme	cell	1068:1071	arg1	migration					1073:1081	cell migration	1068:1081	cell migration	1068:1081	We find that inclusion of the dorsal HA surface induces formation of adhesive complexes and significantly slows cell migration relative to a free fibronectin-coated surface.
25047626	5	49	theme	surface	996:1002	arg1	inclusion					969:977	inclusion	969:977	inclusion of the dorsal HA surface	969:1002	We find that inclusion of the dorsal HA surface induces formation of adhesive complexes and significantly slows cell migration relative to a free fibronectin-coated surface.
25047626	1	50	theme	diffuse	187:193	arg1	infiltration					195:206	diffuse infiltration	187:206	diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence	187:322	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	2	51	theme	topological	435:445	arg1	interfaces					447:456	topological interfaces	435:456	topological interfaces thought to serve as "tracks" that speed cell migration	435:511	The most rapid mode of tissue infiltration occurs along blood vessels or white matter tracts, which represent topological interfaces thought to serve as "tracks" that speed cell migration.
25047626	7	52	theme	dorsal	1634:1639	arg1	surface					1641:1647	the dorsal surface	1630:1647	the dorsal surface	1630:1647	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	6	53	theme	specific	1319:1326	arg1	mechanisms					1328:1337	biochemically specific mechanisms	1305:1337	biochemically specific mechanisms rather than simple steric hindrance	1305:1373	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	4	54	from	sandwiched	787:796	arg1	system					755:760	a culture system	745:760	a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma	745:953	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	5	55	theme	free	1097:1100	arg1	surface					1121:1127	a free fibronectin-coated surface	1095:1127	a free fibronectin-coated surface	1095:1127	We find that inclusion of the dorsal HA surface induces formation of adhesive complexes and significantly slows cell migration relative to a free fibronectin-coated surface.
25047626	3	56	theme	reductionist	642:653	arg1	dissection					655:664	reductionist dissection	642:664	reductionist dissection of mechanisms of this interfacial motility	642:707	Despite this observation, the field lacks experimental paradigms that capture key features of these tissue interfaces and allow reductionist dissection of mechanisms of this interfacial motility.
25047626	5	57	theme	dorsal	986:991	arg1	surface					996:1002	the dorsal HA surface	982:1002	the dorsal HA surface	982:1002	We find that inclusion of the dorsal HA surface induces formation of adhesive complexes and significantly slows cell migration relative to a free fibronectin-coated surface.
25047626	4	58	theme	hyaluronic	896:905	arg1	HA					913:914	HA	913:914	HA	913:914	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	4	58	theme	hyaluronic	896:905	arg1	acid					907:910	hyaluronic acid	896:910	a dorsal hyaluronic acid (HA) surface	887:923	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	5	59	theme	adhesive	1025:1032	arg1	complexes					1034:1042	adhesive complexes	1025:1042	adhesive complexes	1025:1042	We find that inclusion of the dorsal HA surface induces formation of adhesive complexes and significantly slows cell migration relative to a free fibronectin-coated surface.
25047626	3	60	theme	key	592:594	arg1	features					596:603	key features	592:603	key features of these tissue interfaces	592:630	Despite this observation, the field lacks experimental paradigms that capture key features of these tissue interfaces and allow reductionist dissection of mechanisms of this interfacial motility.
25047626	4	61	theme	dorsal	889:894	arg1	surface					917:923	a dorsal hyaluronic acid (HA) surface	887:923	a dorsal hyaluronic acid (HA) surface	887:923	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	0	62	from	interfaces	83:92	arg1	dissection					38:47	the dissection	34:47	the dissection of tumor cell migration at tissue interfaces	34:92	A composite hydrogel platform for the dissection of tumor cell migration at tissue interfaces.
25047626	6	63	theme	binding	1185:1191	arg1	peptides					1193:1200	integrin binding peptides	1176:1200	integrin binding peptides	1176:1200	This retardation is amplified by inclusion of integrin binding peptides in the dorsal layer and expression of CD44, suggesting that the dorsal surface slows migration through biochemically specific mechanisms rather than simple steric hindrance.
25047626	7	64	theme	adhesive	1608:1615	arg1	signaling					1617:1625	adhesive signaling	1608:1625	adhesive signaling	1608:1625	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	0	65	theme	cell	58:61	arg1	migration					63:71	tumor cell migration	52:71	tumor cell migration	52:71	A composite hydrogel platform for the dissection of tumor cell migration at tissue interfaces.
25047626	2	66	theme	blood	381:385	arg1	vessels					387:393	blood vessels	381:393	blood vessels	381:393	The most rapid mode of tissue infiltration occurs along blood vessels or white matter tracts, which represent topological interfaces thought to serve as "tracks" that speed cell migration.
25047626	4	67	theme	tumor	771:775	arg1	cells					777:781	tumor cells	771:781	tumor cells	771:781	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	7	68	from	reduction	1395:1403	arg1	speed					1418:1422	migration speed	1408:1422	migration speed	1408:1422	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	1	69	theme	tumor	211:215	arg1	cells					217:221	tumor cells	211:221	tumor cells	211:221	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	0	70	theme	tumor	52:56	arg1	migration					63:71	tumor cell migration	52:71	tumor cell migration	52:71	A composite hydrogel platform for the dissection of tumor cell migration at tissue interfaces.
25047626	1	71	theme	tumor	307:311	arg1	recurrence					313:322	tumor recurrence	307:322	tumor recurrence	307:322	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
25047626	0	72	theme	hydrogel	12:19	arg1	platform					21:28	A composite hydrogel platform	0:28	A composite hydrogel platform for the dissection of tumor cell migration at tissue interfaces	0:92	A composite hydrogel platform for the dissection of tumor cell migration at tissue interfaces.
25047626	4	73	theme	brain	938:942	arg1	parenchyma					944:953	brain parenchyma	938:953	brain parenchyma	938:953	To address this need, we developed a culture system in which tumor cells are sandwiched between a fibronectin-coated ventral surface representing vascular basement membrane and a dorsal hyaluronic acid (HA) surface representing brain parenchyma.
25047626	7	74	from	assembly	1428:1435	arg1	speed					1418:1422	migration speed	1408:1422	migration speed	1408:1422	Moreover, both the reduction in migration speed and assembly of dorsal adhesions depend on myosin activation and the stiffness of the ventral layer, implying that mechanochemical feedback directed by the ventral layer can influence adhesive signaling at the dorsal surface.
25047626	1	75	theme	cells	217:221	arg1	infiltration					195:206	diffuse infiltration	187:206	diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence	187:322	Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence.
28339105	0	0	theme	Sensory	71:77	arg1	Properties					79:88	Physical and Sensory Properties	58:88	Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia)	58:143	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	0	0	theme	Sensory	71:77	arg1	Digestibility					43:55	In Vitro Digestibility	34:55	In Vitro Digestibility	34:55	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	3	1	from	alternative	390:400	arg1	context					348:354	this context	343:354	this context	343:354	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	5	2	theme	screw	730:734	arg1	250 rpm					750:756	250 rpm	750:756	250 rpm	750:756	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	2	theme	screw	730:734	arg1	speed					736:740	screw speed	730:740	screw speed (100 to 250 rpm)	730:757	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	9	3	theme	experimental	1256:1267	arg1	conditions					1269:1278	The experimental conditions	1252:1278	The experimental conditions of this study	1252:1292	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	8	4	theme	L	1130:1130	arg1	luminosity					1118:1127	the luminosity	1114:1127	the luminosity (L* )	1114:1133	An increase in moisture positively affected the hardness and the luminosity (L* ), although it negatively affected the volumetric expansion index, crispness, and color parameters (a* , b* , and ΔE).
28339105	8	4	theme	L	1130:1130	arg1	*					1131:1131	L*	1130:1131	L*	1130:1131	An increase in moisture positively affected the hardness and the luminosity (L* ), although it negatively affected the volumetric expansion index, crispness, and color parameters (a* , b* , and ΔE).
28339105	6	5	theme	structural	821:830	arg1	characteristics					832:846	The structural characteristics	817:846	The structural characteristics	817:846	The structural characteristics, in vitro digestibility and sensory acceptance were also evaluated.
28339105	1	6	theme	Brazilian	146:154	arg1	pinhão					168:173	pinhão	168:173	pinhão	168:173	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	1	6	theme	Brazilian	146:154	arg1	seeds					161:165	Brazilian pine seeds	146:165	Brazilian pine seeds (pinhão)	146:174	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	1	6	theme	Brazilian	146:154	arg1	products					192:199	gluten-free products	180:199	gluten-free products derived from Araucaria angustifolia	180:235	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	5	7	from	content	702:708	arg1	°C					812:813	120 to 200 °C	801:813	120 to 200 °C	801:813	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	7	from	content	702:708	arg1	zone					795:798	the 3rd heating zone	779:798	the 3rd heating zone (120 to 200 °C)	779:814	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	0	8	from	Influence	0:8	arg1	Properties					79:88	Physical and Sensory Properties	58:88	Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia)	58:143	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	0	8	from	Influence	0:8	arg1	Digestibility					43:55	In Vitro Digestibility	34:55	In Vitro Digestibility	34:55	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	8	9	theme	b	1238:1238	arg1	*					1239:1239	b*	1238:1239	b*	1238:1239	An increase in moisture positively affected the hardness and the luminosity (L* ), although it negatively affected the volumetric expansion index, crispness, and color parameters (a* , b* , and ΔE).
28339105	7	10	dep	starch	930:935	arg1	contents					937:944	contents	937:944	The resistant starch contents	916:944	The resistant starch contents is almost reduced to zero after extrusion cooking while the slowly digestible starch content is increased.
28339105	5	11	dep	considered	681:690	arg1	temperature					764:774	temperature	764:774	temperature in the 3rd heating zone (120 to 200 °C)	764:814	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	11	dep	considered	681:690	arg1	content					702:708	moisture content	693:708	moisture content (14 to 22 g/100 g)	693:727	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	11	dep	considered	681:690	arg1	22 g/100 g					717:726	22 g/100 g	717:726	22 g/100 g	717:726	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	11	dep	considered	681:690	arg1	250 rpm					750:756	250 rpm	750:756	250 rpm	750:756	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	11	dep	considered	681:690	arg1	speed					736:740	screw speed	730:740	screw speed (100 to 250 rpm)	730:757	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	8	12	theme	expansion	1183:1191	arg1	index					1193:1197	the volumetric expansion index	1168:1197	the volumetric expansion index	1168:1197	An increase in moisture positively affected the hardness and the luminosity (L* ), although it negatively affected the volumetric expansion index, crispness, and color parameters (a* , b* , and ΔE).
28339105	0	13	theme	Pine	103:106	arg1	Flour					114:118	Brazilian Pine Seeds Flour	93:118	Brazilian Pine Seeds Flour (Araucaria Angustifolia)	93:143	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	0	13	theme	Pine	103:106	arg1	Angustifolia					131:142	Angustifolia	131:142	Angustifolia	131:142	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	5	14	from	temperature	764:774	arg1	°C					812:813	120 to 200 °C	801:813	120 to 200 °C	801:813	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	14	from	temperature	764:774	arg1	zone					795:798	the 3rd heating zone	779:798	the 3rd heating zone (120 to 200 °C)	779:814	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	7	15	theme	starch	1024:1029	arg1	content					1031:1037	the slowly digestible starch content	1002:1037	the slowly digestible starch content	1002:1037	The resistant starch contents is almost reduced to zero after extrusion cooking while the slowly digestible starch content is increased.
28339105	8	16	theme	volumetric	1172:1181	arg1	index					1193:1197	the volumetric expansion index	1168:1197	the volumetric expansion index	1168:1197	An increase in moisture positively affected the hardness and the luminosity (L* ), although it negatively affected the volumetric expansion index, crispness, and color parameters (a* , b* , and ΔE).
28339105	2	17	theme	seeds	269:273	arg1	commercialization					242:258	The commercialization	238:258	The commercialization of these seeds	238:273	The commercialization of these seeds is essentially associated with a low level of industrialization.
28339105	1	18	theme	pine	156:159	arg1	pinhão					168:173	pinhão	168:173	pinhão	168:173	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	1	18	theme	pine	156:159	arg1	seeds					161:165	Brazilian pine seeds	146:165	Brazilian pine seeds (pinhão)	146:174	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	1	18	theme	pine	156:159	arg1	products					192:199	gluten-free products	180:199	gluten-free products derived from Araucaria angustifolia	180:235	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	0	19	theme	Brazilian	93:101	arg1	Flour					114:118	Brazilian Pine Seeds Flour	93:118	Brazilian Pine Seeds Flour (Araucaria Angustifolia)	93:143	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	0	19	theme	Brazilian	93:101	arg1	Angustifolia					131:142	Angustifolia	131:142	Angustifolia	131:142	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	5	20	dep	250 rpm	750:756	arg1	to					747:748	to	747:748	to	747:748	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	3	21	theme	human	502:506	arg1	consumption					508:518	human consumption	502:518	human consumption	502:518	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	10	22	theme	extrusion	1439:1447	arg1	method					1485:1490	a potential method	1473:1490	a potential method for the industrialization of Brazilian pine seeds	1473:1540	Thus, extrusion cooking was found to be a potential method for the industrialization of Brazilian pine seeds as a food product.
28339105	10	22	theme	extrusion	1439:1447	arg1	cooking					1449:1455	extrusion cooking	1439:1455	extrusion cooking	1439:1455	Thus, extrusion cooking was found to be a potential method for the industrialization of Brazilian pine seeds as a food product.
28339105	3	23	theme	extrusion	357:365	arg1	alternative					390:400	a potential alternative	378:400	a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption	378:518	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	3	23	theme	extrusion	357:365	arg1	cooking					367:373	extrusion cooking	357:373	extrusion cooking	357:373	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	2	24	theme	low	308:310	arg1	level					312:316	a low level	306:316	a low level of industrialization	306:337	The commercialization of these seeds is essentially associated with a low level of industrialization.
28339105	9	25	theme	expanded	1320:1327	arg1	extrudates					1329:1338	expanded extrudates	1320:1338	expanded extrudates from Brazilian pine seeds	1320:1364	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	7	26	theme	extrusion	978:986	arg1	cooking					988:994	extrusion cooking	978:994	extrusion cooking	978:994	The resistant starch contents is almost reduced to zero after extrusion cooking while the slowly digestible starch content is increased.
28339105	4	27	theme	pine	531:534	arg1	flour					542:546	Brazilian pine seeds flour	521:546	Brazilian pine seeds flour	521:546	Brazilian pine seeds flour was processed in a single-screw extruder following a central composite rotatable design.
28339105	7	28	theme	digestible	1013:1022	arg1	starch					1024:1029	the slowly digestible starch	1002:1029	the slowly digestible starch content	1002:1037	The resistant starch contents is almost reduced to zero after extrusion cooking while the slowly digestible starch content is increased.
28339105	6	29	dep	in	849:850	arg1	vitro					852:856	vitro	852:856	vitro	852:856	The structural characteristics, in vitro digestibility and sensory acceptance were also evaluated.
28339105	0	30	theme	Cooking	23:29	arg1	Influence					0:8	Influence	0:8	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).	0:144	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	4	31	theme	Brazilian	521:529	arg1	flour					542:546	Brazilian pine seeds flour	521:546	Brazilian pine seeds flour	521:546	Brazilian pine seeds flour was processed in a single-screw extruder following a central composite rotatable design.
28339105	0	32	theme	Flour	114:118	arg1	Properties					79:88	Physical and Sensory Properties	58:88	Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia)	58:143	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	0	32	theme	Flour	114:118	arg1	Digestibility					43:55	In Vitro Digestibility	34:55	In Vitro Digestibility	34:55	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	5	33	dep	200	808:810	arg1	to					805:806	to	805:806	to	805:806	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	4	34	theme	composite	609:617	arg1	design					629:634	a central composite rotatable design	599:634	a central composite rotatable design	599:634	Brazilian pine seeds flour was processed in a single-screw extruder following a central composite rotatable design.
28339105	0	35	theme	Extrusion	13:21	arg1	Cooking					23:29	Extrusion Cooking	13:29	Extrusion Cooking	13:29	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	9	36	theme	pine	1355:1358	arg1	seeds					1360:1364	Brazilian pine seeds	1345:1364	Brazilian pine seeds	1345:1364	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	0	37	theme	Seeds	108:112	arg1	Flour					114:118	Brazilian Pine Seeds Flour	93:118	Brazilian Pine Seeds Flour (Araucaria Angustifolia)	93:143	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	0	37	theme	Seeds	108:112	arg1	Angustifolia					131:142	Angustifolia	131:142	Angustifolia	131:142	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	8	38	theme	a	1233:1233	arg1	*					1234:1234	a*	1233:1234	a*	1233:1234	An increase in moisture positively affected the hardness and the luminosity (L* ), although it negatively affected the volumetric expansion index, crispness, and color parameters (a* , b* , and ΔE).
28339105	4	39	theme	central	601:607	arg1	design					629:634	a central composite rotatable design	599:634	a central composite rotatable design	599:634	Brazilian pine seeds flour was processed in a single-screw extruder following a central composite rotatable design.
28339105	0	40	theme	In	34:35	arg1	Properties					79:88	Physical and Sensory Properties	58:88	Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia)	58:143	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	0	40	theme	In	34:35	arg1	Digestibility					43:55	In Vitro Digestibility	34:55	In Vitro Digestibility	34:55	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	4	41	theme	seeds	536:540	arg1	flour					542:546	Brazilian pine seeds flour	521:546	Brazilian pine seeds flour	521:546	Brazilian pine seeds flour was processed in a single-screw extruder following a central composite rotatable design.
28339105	5	42	theme	moisture	693:700	arg1	22 g/100 g					717:726	22 g/100 g	717:726	22 g/100 g	717:726	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	42	theme	moisture	693:700	arg1	content					702:708	moisture content	693:708	moisture content (14 to 22 g/100 g)	693:727	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	43	theme	3rd	783:785	arg1	°C					812:813	120 to 200 °C	801:813	120 to 200 °C	801:813	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	43	theme	3rd	783:785	arg1	zone					795:798	the 3rd heating zone	779:798	the 3rd heating zone (120 to 200 °C)	779:814	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	6	44	theme	sensory	876:882	arg1	acceptance					884:893	sensory acceptance	876:893	sensory acceptance	876:893	The structural characteristics, in vitro digestibility and sensory acceptance were also evaluated.
28339105	9	45	theme	extrudates	1329:1338	arg1	production					1306:1315	the production	1302:1315	the production of expanded extrudates from Brazilian pine seeds	1302:1364	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	1	46	theme	gluten-free	180:190	arg1	seeds					161:165	Brazilian pine seeds	146:165	Brazilian pine seeds (pinhão)	146:174	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	1	46	theme	gluten-free	180:190	arg1	products					192:199	gluten-free products	180:199	gluten-free products derived from Araucaria angustifolia	180:235	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	3	47	theme	pinhão	430:435	arg1	extrudates					416:425	extrudates	416:425	extrudates of pinhão	416:435	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	3	47	theme	pinhão	430:435	arg1	product					447:453	a food product	440:453	a food product	440:453	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	3	47	theme	pinhão	430:435	arg1	ready					492:496	ready	492:496	ready	492:496	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	9	48	theme	study	1288:1292	arg1	conditions					1269:1278	The experimental conditions	1252:1278	The experimental conditions of this study	1252:1292	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	1	49	attach	derived	201:207	arg2	products					192:199	gluten-free products	180:199	gluten-free products derived from Araucaria angustifolia	180:235	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	1	49	attach	derived	201:207	arg1	angustifolia					224:235	Araucaria angustifolia	214:235	Araucaria angustifolia	214:235	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	1	49	attach	derived	201:207	arg2	seeds					161:165	Brazilian pine seeds	146:165	Brazilian pine seeds (pinhão)	146:174	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	5	50	theme	heating	787:793	arg1	°C					812:813	120 to 200 °C	801:813	120 to 200 °C	801:813	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	50	theme	heating	787:793	arg1	zone					795:798	the 3rd heating zone	779:798	the 3rd heating zone (120 to 200 °C)	779:814	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	3	51	theme	food	442:445	arg1	extrudates					416:425	extrudates	416:425	extrudates of pinhão	416:435	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	3	51	theme	food	442:445	arg1	product					447:453	a food product	440:453	a food product	440:453	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	3	51	theme	food	442:445	arg1	ready					492:496	ready	492:496	ready	492:496	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	3	52	theme	potential	380:388	arg1	alternative					390:400	a potential alternative	378:400	a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption	378:518	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	3	52	theme	potential	380:388	arg1	cooking					367:373	extrusion cooking	357:373	extrusion cooking	357:373	In this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption.
28339105	5	53	theme	independent	652:662	arg1	factors					643:649	Three factors	637:649	Three factors (independent parameters)	637:674	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	53	theme	independent	652:662	arg1	parameters					664:673	independent parameters	652:673	independent parameters	652:673	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	8	54	from	increase	1056:1063	arg1	moisture					1068:1075	moisture	1068:1075	moisture	1068:1075	An increase in moisture positively affected the hardness and the luminosity (L* ), although it negatively affected the volumetric expansion index, crispness, and color parameters (a* , b* , and ΔE).
28339105	0	55	dep	In	34:35	arg1	Vitro					37:41	Vitro	37:41	Vitro	37:41	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	9	56	from	seeds	1360:1364	arg1	production					1306:1315	the production	1302:1315	the production of expanded extrudates from Brazilian pine seeds	1302:1364	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	9	56	from	seeds	1360:1364	arg1	extrudates					1329:1338	expanded extrudates	1320:1338	expanded extrudates from Brazilian pine seeds	1320:1364	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	10	57	theme	potential	1475:1483	arg1	method					1485:1490	a potential method	1473:1490	a potential method for the industrialization of Brazilian pine seeds	1473:1540	Thus, extrusion cooking was found to be a potential method for the industrialization of Brazilian pine seeds as a food product.
28339105	10	57	theme	potential	1475:1483	arg1	cooking					1449:1455	extrusion cooking	1439:1455	extrusion cooking	1439:1455	Thus, extrusion cooking was found to be a potential method for the industrialization of Brazilian pine seeds as a food product.
28339105	5	58	dep	22 g/100 g	717:726	arg1	to					714:715	to	714:715	to	714:715	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	8	59	dep	parameters	1221:1230	arg1	*					1239:1239	b*	1238:1239	b*	1238:1239	An increase in moisture positively affected the hardness and the luminosity (L* ), although it negatively affected the volumetric expansion index, crispness, and color parameters (a* , b* , and ΔE).
28339105	8	59	dep	parameters	1221:1230	arg1	ΔE					1247:1248	ΔE	1247:1248	ΔE	1247:1248	An increase in moisture positively affected the hardness and the luminosity (L* ), although it negatively affected the volumetric expansion index, crispness, and color parameters (a* , b* , and ΔE).
28339105	8	59	dep	parameters	1221:1230	arg1	*					1234:1234	a*	1233:1234	a*	1233:1234	An increase in moisture positively affected the hardness and the luminosity (L* ), although it negatively affected the volumetric expansion index, crispness, and color parameters (a* , b* , and ΔE).
28339105	9	60	theme	Brazilian	1345:1353	arg1	seeds					1360:1364	Brazilian pine seeds	1345:1364	Brazilian pine seeds	1345:1364	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	2	61	theme	industrialization	321:337	arg1	level					312:316	a low level	306:316	a low level of industrialization	306:337	The commercialization of these seeds is essentially associated with a low level of industrialization.
28339105	6	62	theme	in	849:850	arg1	digestibility					858:870	in vitro digestibility	849:870	in vitro digestibility	849:870	The structural characteristics, in vitro digestibility and sensory acceptance were also evaluated.
28339105	5	63	from	speed	736:740	arg1	°C					812:813	120 to 200 °C	801:813	120 to 200 °C	801:813	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	5	63	from	speed	736:740	arg1	zone					795:798	the 3rd heating zone	779:798	the 3rd heating zone (120 to 200 °C)	779:814	Three factors (independent parameters) were considered: moisture content (14 to 22 g/100 g), screw speed (100 to 250 rpm), and temperature in the 3rd heating zone (120 to 200 °C).
28339105	8	64	theme	color	1215:1219	arg1	parameters					1221:1230	color parameters	1215:1230	color parameters (a* , b* , and ΔE)	1215:1249	An increase in moisture positively affected the hardness and the luminosity (L* ), although it negatively affected the volumetric expansion index, crispness, and color parameters (a* , b* , and ΔE).
28339105	9	65	theme	acceptance	1411:1420	arg1	qualities					1422:1430	acceptance qualities	1411:1430	acceptance qualities	1411:1430	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	10	66	theme	seeds	1536:1540	arg1	industrialization					1500:1516	the industrialization	1496:1516	the industrialization of Brazilian pine seeds	1496:1540	Thus, extrusion cooking was found to be a potential method for the industrialization of Brazilian pine seeds as a food product.
28339105	10	67	theme	food	1547:1550	arg1	product					1552:1558	a food product	1545:1558	a food product	1545:1558	Thus, extrusion cooking was found to be a potential method for the industrialization of Brazilian pine seeds as a food product.
28339105	4	68	theme	single-screw	567:578	arg1	extruder					580:587	a single-screw extruder	565:587	a single-screw extruder following a central composite rotatable design	565:634	Brazilian pine seeds flour was processed in a single-screw extruder following a central composite rotatable design.
28339105	0	69	theme	Physical	58:65	arg1	Properties					79:88	Physical and Sensory Properties	58:88	Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia)	58:143	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	0	69	theme	Physical	58:65	arg1	Digestibility					43:55	In Vitro Digestibility	34:55	In Vitro Digestibility	34:55	Influence of Extrusion Cooking on In Vitro Digestibility, Physical and Sensory Properties of Brazilian Pine Seeds Flour (Araucaria Angustifolia).
28339105	4	70	theme	rotatable	619:627	arg1	design					629:634	a central composite rotatable design	599:634	a central composite rotatable design	599:634	Brazilian pine seeds flour was processed in a single-screw extruder following a central composite rotatable design.
28339105	7	71	theme	resistant	920:928	arg1	starch					930:935	The resistant starch	916:935	The resistant starch contents	916:944	The resistant starch contents is almost reduced to zero after extrusion cooking while the slowly digestible starch content is increased.
28339105	9	72	theme	texture	1387:1393	arg1	properties					1395:1404	texture properties	1387:1404	texture properties	1387:1404	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	10	73	theme	Brazilian	1521:1529	arg1	seeds					1536:1540	Brazilian pine seeds	1521:1540	Brazilian pine seeds	1521:1540	Thus, extrusion cooking was found to be a potential method for the industrialization of Brazilian pine seeds as a food product.
28339105	1	74	theme	Araucaria	214:222	arg1	angustifolia					224:235	Araucaria angustifolia	214:235	Araucaria angustifolia	214:235	Brazilian pine seeds (pinhão) are gluten-free products derived from Araucaria angustifolia.
28339105	9	75	theme	good	1371:1374	arg1	expansion					1376:1384	good expansion	1371:1384	good expansion	1371:1384	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	9	76	from	production	1306:1315	arg1	seeds					1360:1364	Brazilian pine seeds	1345:1364	Brazilian pine seeds	1345:1364	The experimental conditions of this study allowed the production of expanded extrudates from Brazilian pine seeds with good expansion, texture properties, and acceptance qualities.
28339105	10	77	theme	pine	1531:1534	arg1	seeds					1536:1540	Brazilian pine seeds	1521:1540	Brazilian pine seeds	1521:1540	Thus, extrusion cooking was found to be a potential method for the industrialization of Brazilian pine seeds as a food product.
26794756	6	0	theme	swelling	929:936	arg1	ratio					938:942	the swelling ratio	925:942	the swelling ratio of GO-RCE/PVA ternary hydrogel	925:973	With 0.8wt% GO loading, the swelling ratio of GO-RCE/PVA ternary hydrogel was improved from 150% (pH=2) to 310% (pH=14).
26794756	7	1	theme	slight	1037:1042	arg1	increase					1044:1051	a slight increase	1035:1051	a slight increase in the water content of the ternary hydrogel	1035:1096	In addition, a slight increase in the water content of the ternary hydrogel was achieved with increasing concentrations of GO.
26794756	1	2	theme	alcohol	195:201	arg1	hydrogels					224:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	2	3	from	effect	339:344	arg1	properties					378:387	the mechanical properties	363:387	the mechanical properties	363:387	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	2	3	from	effect	339:344	arg1	behavior					399:406	swelling behavior	390:406	swelling behavior	390:406	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	2	3	from	effect	339:344	arg1	content					415:421	water content	409:421	water content of composite hydrogels	409:444	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	1	4	theme	thawing	289:295	arg1	method					297:302	a repeated freezing and thawing method	265:302	a repeated freezing and thawing method in NaOH/urea aqueous solution	265:332	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	0	5	theme	pH-sensitive	99:110	arg1	behavior					112:119	pH-sensitive behavior	99:119	pH-sensitive behavior	99:119	Preparation and properties of graphene oxide-regenerated cellulose/polyvinyl alcohol hydrogel with pH-sensitive behavior.
26794756	5	6	theme	higher	878:883	arg1	ratio					894:898	higher swelling ratio	878:898	higher swelling ratio	878:898	Meanwhile, GO-RCE/PVA ternary hydrogels performed the excellent pH-sensitivity, and the higher pH leaded to higher swelling ratio.
26794756	4	7	dep	%	758:758	arg1	to					760:761	to	760:761	to	760:761	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	4	8	theme	%	619:619	arg1	addition					602:609	the addition	598:609	the addition of 1.0wt% GO	598:622	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	6	9	dep	loading	916:922	arg1	GO					913:914	GO	913:914	GO	913:914	With 0.8wt% GO loading, the swelling ratio of GO-RCE/PVA ternary hydrogel was improved from 150% (pH=2) to 310% (pH=14).
26794756	8	10	theme	engineering	1255:1265	arg1	devices					1283:1289	biomedical engineering and intelligent devices	1244:1289	biomedical engineering and intelligent devices	1244:1289	It is believed that this novel ternary hydrogels is a promising material in the application of biomedical engineering and intelligent devices.
26794756	6	11	theme	ternary	958:964	arg1	hydrogel					966:973	GO-RCE/PVA ternary hydrogel	947:973	GO-RCE/PVA ternary hydrogel	947:973	With 0.8wt% GO loading, the swelling ratio of GO-RCE/PVA ternary hydrogel was improved from 150% (pH=2) to 310% (pH=14).
26794756	7	12	theme	increasing	1116:1125	arg1	concentrations					1127:1140	increasing concentrations	1116:1140	increasing concentrations of GO	1116:1146	In addition, a slight increase in the water content of the ternary hydrogel was achieved with increasing concentrations of GO.
26794756	8	13	theme	biomedical	1244:1253	arg1	devices					1283:1289	biomedical engineering and intelligent devices	1244:1289	biomedical engineering and intelligent devices	1244:1289	It is believed that this novel ternary hydrogels is a promising material in the application of biomedical engineering and intelligent devices.
26794756	2	14	theme	swelling	390:397	arg1	properties					378:387	the mechanical properties	363:387	the mechanical properties	363:387	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	2	14	theme	swelling	390:397	arg1	behavior					399:406	swelling behavior	390:406	swelling behavior	390:406	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	1	15	theme	NaOH/urea	307:315	arg1	solution					325:332	NaOH/urea aqueous solution	307:332	NaOH/urea aqueous solution	307:332	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	5	16	dep	Meanwhile	770:778	arg1	ternary					792:798	ternary	792:798	ternary	792:798	Meanwhile, GO-RCE/PVA ternary hydrogels performed the excellent pH-sensitivity, and the higher pH leaded to higher swelling ratio.
26794756	3	17	theme	RCE/PVA	574:580	arg1	hydrogels					582:590	RCE/PVA hydrogels	574:590	RCE/PVA hydrogels	574:590	It was found that the mechanical properties of GO-RCE/PVA ternary hydrogels were largely enhanced relative to RCE/PVA hydrogels.
26794756	5	18	theme	higher	858:863	arg1	pH					865:866	the higher pH	854:866	the higher pH	854:866	Meanwhile, GO-RCE/PVA ternary hydrogels performed the excellent pH-sensitivity, and the higher pH leaded to higher swelling ratio.
26794756	1	19	theme	GO-RCE/PVA	204:213	arg1	hydrogels					224:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	6	20	theme	GO-RCE/PVA	947:956	arg1	hydrogel					966:973	GO-RCE/PVA ternary hydrogel	947:973	GO-RCE/PVA ternary hydrogel	947:973	With 0.8wt% GO loading, the swelling ratio of GO-RCE/PVA ternary hydrogel was improved from 150% (pH=2) to 310% (pH=14).
26794756	4	21	theme	238	763:765	arg1	%					758:758	%	758:758	%	758:758	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	2	22	dep	content	352:358	arg1	GO					349:350	GO	349:350	GO	349:350	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	7	23	theme	ternary	1081:1087	arg1	hydrogel					1089:1096	the ternary hydrogel	1077:1096	the ternary hydrogel	1077:1096	In addition, a slight increase in the water content of the ternary hydrogel was achieved with increasing concentrations of GO.
26794756	2	24	theme	content	352:358	arg1	effect					339:344	The effect	335:344	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels	335:444	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	4	25	from	0.52MPa	674:680	arg1	%					667:667	40.4%	663:667	40.4% from 0.52MPa to 0.73MPa	663:691	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	1	26	theme	ternary	216:222	arg1	hydrogels					224:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	7	27	theme	hydrogel	1089:1096	arg1	content					1066:1072	the water content	1056:1072	the water content of the ternary hydrogel	1056:1096	In addition, a slight increase in the water content of the ternary hydrogel was achieved with increasing concentrations of GO.
26794756	1	28	theme	aqueous	317:323	arg1	solution					325:332	NaOH/urea aqueous solution	307:332	NaOH/urea aqueous solution	307:332	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	4	29	theme	elongation	729:738	arg1	increase					713:720	the increase	709:720	the increase of the elongation at break	709:747	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	8	30	theme	devices	1283:1289	arg1	application					1229:1239	the application	1225:1239	the application of biomedical engineering and intelligent devices	1225:1289	It is believed that this novel ternary hydrogels is a promising material in the application of biomedical engineering and intelligent devices.
26794756	2	31	theme	water	409:413	arg1	properties					378:387	the mechanical properties	363:387	the mechanical properties	363:387	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	2	31	theme	water	409:413	arg1	content					415:421	water content	409:421	water content of composite hydrogels	409:444	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	4	32	theme	tensile	629:635	arg1	strength					637:644	the tensile strength	625:644	the tensile strength	625:644	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	8	33	theme	promising	1203:1211	arg1	material					1213:1220	a promising material	1201:1220	a promising material in the application of biomedical engineering and intelligent devices	1201:1289	It is believed that this novel ternary hydrogels is a promising material in the application of biomedical engineering and intelligent devices.
26794756	8	33	theme	promising	1203:1211	arg1	hydrogels					1188:1196	this novel ternary hydrogels	1169:1196	this novel ternary hydrogels	1169:1196	It is believed that this novel ternary hydrogels is a promising material in the application of biomedical engineering and intelligent devices.
26794756	4	34	from	break	743:747	arg1	increase					713:720	the increase	709:720	the increase of the elongation at break	709:747	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	4	34	from	break	743:747	arg1	elongation					729:738	the elongation	725:738	the elongation at break	725:747	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	4	35	theme	103	755:757	arg1	%					758:758	%	758:758	%	758:758	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	8	36	from	material	1213:1220	arg1	application					1229:1239	the application	1225:1239	the application of biomedical engineering and intelligent devices	1225:1289	It is believed that this novel ternary hydrogels is a promising material in the application of biomedical engineering and intelligent devices.
26794756	8	37	theme	novel	1174:1178	arg1	material					1213:1220	a promising material	1201:1220	a promising material in the application of biomedical engineering and intelligent devices	1201:1289	It is believed that this novel ternary hydrogels is a promising material in the application of biomedical engineering and intelligent devices.
26794756	8	37	theme	novel	1174:1178	arg1	hydrogels					1188:1196	this novel ternary hydrogels	1169:1196	this novel ternary hydrogels	1169:1196	It is believed that this novel ternary hydrogels is a promising material in the application of biomedical engineering and intelligent devices.
26794756	4	38	theme	1.0wt	614:618	arg1	%					619:619	1.0wt% GO	614:622	1.0wt% GO	614:622	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	8	39	theme	intelligent	1271:1281	arg1	devices					1283:1289	biomedical engineering and intelligent devices	1244:1289	biomedical engineering and intelligent devices	1244:1289	It is believed that this novel ternary hydrogels is a promising material in the application of biomedical engineering and intelligent devices.
26794756	5	40	theme	swelling	885:892	arg1	ratio					894:898	higher swelling ratio	878:898	higher swelling ratio	878:898	Meanwhile, GO-RCE/PVA ternary hydrogels performed the excellent pH-sensitivity, and the higher pH leaded to higher swelling ratio.
26794756	5	41	theme	excellent	824:832	arg1	pH-sensitivity					834:847	the excellent pH-sensitivity	820:847	the excellent pH-sensitivity	820:847	Meanwhile, GO-RCE/PVA ternary hydrogels performed the excellent pH-sensitivity, and the higher pH leaded to higher swelling ratio.
26794756	7	42	theme	water	1060:1064	arg1	content					1066:1072	the water content	1056:1072	the water content of the ternary hydrogel	1056:1096	In addition, a slight increase in the water content of the ternary hydrogel was achieved with increasing concentrations of GO.
26794756	2	43	theme	mechanical	367:376	arg1	properties					378:387	the mechanical properties	363:387	the mechanical properties	363:387	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	2	43	theme	mechanical	367:376	arg1	behavior					399:406	swelling behavior	390:406	swelling behavior	390:406	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	2	43	theme	mechanical	367:376	arg1	content					415:421	water content	409:421	water content of composite hydrogels	409:444	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	0	44	theme	graphene	30:37	arg1	properties					16:25	properties	16:25	properties	16:25	Preparation and properties of graphene oxide-regenerated cellulose/polyvinyl alcohol hydrogel with pH-sensitive behavior.
26794756	0	44	theme	graphene	30:37	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of graphene oxide-regenerated cellulose/polyvinyl alcohol hydrogel with pH-sensitive behavior.
26794756	1	45	from	method	297:302	arg1	solution					325:332	NaOH/urea aqueous solution	307:332	NaOH/urea aqueous solution	307:332	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	6	46	theme	hydrogel	966:973	arg1	ratio					938:942	the swelling ratio	925:942	the swelling ratio of GO-RCE/PVA ternary hydrogel	925:973	With 0.8wt% GO loading, the swelling ratio of GO-RCE/PVA ternary hydrogel was improved from 150% (pH=2) to 310% (pH=14).
26794756	2	47	theme	hydrogels	436:444	arg1	properties					378:387	the mechanical properties	363:387	the mechanical properties	363:387	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	2	47	theme	hydrogels	436:444	arg1	content					415:421	water content	409:421	water content of composite hydrogels	409:444	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	0	48	theme	alcohol	77:83	arg1	hydrogel					85:92	cellulose/polyvinyl alcohol hydrogel	57:92	cellulose/polyvinyl alcohol hydrogel	57:92	Preparation and properties of graphene oxide-regenerated cellulose/polyvinyl alcohol hydrogel with pH-sensitive behavior.
26794756	4	49	dep	%	619:619	arg1	GO					621:622	GO	621:622	GO	621:622	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	7	50	from	increase	1044:1051	arg1	content					1066:1072	the water content	1056:1072	the water content of the ternary hydrogel	1056:1096	In addition, a slight increase in the water content of the ternary hydrogel was achieved with increasing concentrations of GO.
26794756	3	51	theme	hydrogels	530:538	arg1	properties					497:506	the mechanical properties	482:506	the mechanical properties of GO-RCE/PVA ternary hydrogels	482:538	It was found that the mechanical properties of GO-RCE/PVA ternary hydrogels were largely enhanced relative to RCE/PVA hydrogels.
26794756	0	52	theme	cellulose/polyvinyl	57:75	arg1	hydrogel					85:92	cellulose/polyvinyl alcohol hydrogel	57:92	cellulose/polyvinyl alcohol hydrogel	57:92	Preparation and properties of graphene oxide-regenerated cellulose/polyvinyl alcohol hydrogel with pH-sensitive behavior.
26794756	2	53	theme	composite	426:434	arg1	hydrogels					436:444	composite hydrogels	426:444	composite hydrogels	426:444	The effect of GO content on the mechanical properties, swelling behavior, water content of composite hydrogels was investigated.
26794756	3	54	theme	GO-RCE/PVA	511:520	arg1	hydrogels					530:538	GO-RCE/PVA ternary hydrogels	511:538	GO-RCE/PVA ternary hydrogels	511:538	It was found that the mechanical properties of GO-RCE/PVA ternary hydrogels were largely enhanced relative to RCE/PVA hydrogels.
26794756	4	55	dep	increased	650:658	arg1	accompanied					694:704	accompanied	694:704	accompanied by the increase of the elongation at break (from 103% to 238%)	694:767	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	1	56	theme	graphene	137:144	arg1	hydrogels					224:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	6	57	theme	%	911:911	arg1	loading					916:922	0.8wt% GO loading	906:922	0.8wt% GO loading	906:922	With 0.8wt% GO loading, the swelling ratio of GO-RCE/PVA ternary hydrogel was improved from 150% (pH=2) to 310% (pH=14).
26794756	1	58	theme	oxide	146:150	arg1	hydrogels					224:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	8	59	theme	ternary	1180:1186	arg1	material					1213:1220	a promising material	1201:1220	a promising material in the application of biomedical engineering and intelligent devices	1201:1289	It is believed that this novel ternary hydrogels is a promising material in the application of biomedical engineering and intelligent devices.
26794756	8	59	theme	ternary	1180:1186	arg1	hydrogels					1188:1196	this novel ternary hydrogels	1169:1196	this novel ternary hydrogels	1169:1196	It is believed that this novel ternary hydrogels is a promising material in the application of biomedical engineering and intelligent devices.
26794756	4	60	dep	accompanied	694:704	arg1	%					766:766	103% to 238%	755:766	103% to 238%	755:766	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
26794756	1	61	theme	reinforced	152:161	arg1	hydrogels					224:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	1	62	theme	repeated	267:274	arg1	method					297:302	a repeated freezing and thawing method	265:302	a repeated freezing and thawing method in NaOH/urea aqueous solution	265:332	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	7	63	theme	GO	1145:1146	arg1	concentrations					1127:1140	increasing concentrations	1116:1140	increasing concentrations of GO	1116:1146	In addition, a slight increase in the water content of the ternary hydrogel was achieved with increasing concentrations of GO.
26794756	3	64	theme	mechanical	486:495	arg1	properties					497:506	the mechanical properties	482:506	the mechanical properties of GO-RCE/PVA ternary hydrogels	482:538	It was found that the mechanical properties of GO-RCE/PVA ternary hydrogels were largely enhanced relative to RCE/PVA hydrogels.
26794756	1	65	theme	regenerated	163:173	arg1	hydrogels					224:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	3	66	theme	ternary	522:528	arg1	hydrogels					530:538	GO-RCE/PVA ternary hydrogels	511:538	GO-RCE/PVA ternary hydrogels	511:538	It was found that the mechanical properties of GO-RCE/PVA ternary hydrogels were largely enhanced relative to RCE/PVA hydrogels.
26794756	1	67	theme	freezing	276:283	arg1	method					297:302	a repeated freezing and thawing method	265:302	a repeated freezing and thawing method in NaOH/urea aqueous solution	265:332	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	5	68	theme	Meanwhile	770:778	arg1	hydrogels					800:808	Meanwhile, GO-RCE/PVA ternary hydrogels	770:808	Meanwhile, GO-RCE/PVA ternary hydrogels	770:808	Meanwhile, GO-RCE/PVA ternary hydrogels performed the excellent pH-sensitivity, and the higher pH leaded to higher swelling ratio.
26794756	6	69	theme	0.8wt	906:910	arg1	loading					916:922	0.8wt% GO loading	906:922	0.8wt% GO loading	906:922	With 0.8wt% GO loading, the swelling ratio of GO-RCE/PVA ternary hydrogel was improved from 150% (pH=2) to 310% (pH=14).
26794756	1	70	theme	cellulose/polyvinyl	175:193	arg1	hydrogels					224:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels	137:232	In this study, graphene oxide reinforced regenerated cellulose/polyvinyl alcohol (GO-RCE/PVA) ternary hydrogels were successfully prepared via a repeated freezing and thawing method in NaOH/urea aqueous solution.
26794756	4	71	from	increase	713:720	arg1	break					743:747	break	743:747	break	743:747	With the addition of 1.0wt% GO, the tensile strength was increased by 40.4% from 0.52MPa to 0.73MPa, accompanied by the increase of the elongation at break (from 103% to 238%).
27211647	5	0	theme	complex	734:740	arg1	similar					746:752	similar	746:752	similar	746:752	The structure of the PSE/γ-CD inclusion complex was similar to that of ChO/γ-CD, based on differential scanning calorimetry and powder X-ray diffractometry results.
27211647	5	0	theme	complex	734:740	arg1	structure					698:706	The structure	694:706	The structure of the PSE/γ-CD inclusion complex	694:740	The structure of the PSE/γ-CD inclusion complex was similar to that of ChO/γ-CD, based on differential scanning calorimetry and powder X-ray diffractometry results.
27211647	4	1	theme	molar	674:678	arg1	ratio					680:684	a molar ratio	672:684	a molar ratio of 2:5	672:691	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	4	2	theme	components	566:575	arg1	one					555:557	one	555:557	one	555:557	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	4	2	theme	components	566:575	arg1	components					566:575	the components	562:575	the components of PSE	562:582	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	4	2	theme	components	566:575	arg1	oleate					541:546	cholesteryl oleate	529:546	cholesteryl oleate (ChO)	529:552	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	2	3	theme	particle	323:330	arg1	structure					297:305	the structure	293:305	the structure of the PSE/γ-CD particle	293:330	This paper elucidates the structure of the PSE/γ-CD particle.
27211647	3	4	theme	Cryogenic	333:341	arg1	microscopy					361:370	Cryogenic scanning electron microscopy	333:370	Cryogenic scanning electron microscopy	333:370	Cryogenic scanning electron microscopy and contact angle measurements showed that the PSE/γ-CD particles formed a capsule-like structure with a hydrophilic surface.
27211647	4	5	theme	PSE	580:582	arg1	components					566:575	the components	562:575	the components of PSE	562:582	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	3	6	theme	scanning	343:350	arg1	microscopy					361:370	Cryogenic scanning electron microscopy	333:370	Cryogenic scanning electron microscopy	333:370	Cryogenic scanning electron microscopy and contact angle measurements showed that the PSE/γ-CD particles formed a capsule-like structure with a hydrophilic surface.
27211647	4	7	theme	2:5	689:691	arg1	ratio					680:684	a molar ratio	672:684	a molar ratio of 2:5	672:691	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	6	8	theme	hydrophilic	1005:1015	arg1	complex					1036:1042	the hydrophilic PSE/γ-CD inclusion complex	1001:1042	the hydrophilic PSE/γ-CD inclusion complex	1001:1042	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	6	9	theme	complex	1036:1042	arg1	layer					992:996	an outer layer	983:996	an outer layer of the hydrophilic PSE/γ-CD inclusion complex	983:1042	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	4	10	theme	inclusion	641:649	arg1	complex					651:657	a hydrophilic and stoichiometric inclusion complex	608:657	a hydrophilic and stoichiometric inclusion complex with γ-CD	608:667	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	6	11	theme	PSE	954:956	arg1	droplet					958:964	a hydrophobic PSE droplet	940:964	a hydrophobic PSE droplet	940:964	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	4	12	theme	stoichiometric	626:639	arg1	complex					651:657	a hydrophilic and stoichiometric inclusion complex	608:657	a hydrophilic and stoichiometric inclusion complex with γ-CD	608:667	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	0	13	theme	novel	2:6	arg1	structure					21:29	A novel capsule-like structure	0:29	A novel capsule-like structure of micro-sized particles	0:54	A novel capsule-like structure of micro-sized particles formed by phytosterol ester and γ-cyclodextrin in water.
27211647	5	14	theme	PSE/γ-CD	715:722	arg1	complex					734:740	the PSE/γ-CD inclusion complex	711:740	the PSE/γ-CD inclusion complex	711:740	The structure of the PSE/γ-CD inclusion complex was similar to that of ChO/γ-CD, based on differential scanning calorimetry and powder X-ray diffractometry results.
27211647	5	15	theme	diffractometry	835:848	arg1	results					850:856	differential scanning calorimetry and powder X-ray diffractometry results	784:856	differential scanning calorimetry and powder X-ray diffractometry results	784:856	The structure of the PSE/γ-CD inclusion complex was similar to that of ChO/γ-CD, based on differential scanning calorimetry and powder X-ray diffractometry results.
27211647	3	16	theme	contact	376:382	arg1	measurements					390:401	contact angle measurements	376:401	contact angle measurements	376:401	Cryogenic scanning electron microscopy and contact angle measurements showed that the PSE/γ-CD particles formed a capsule-like structure with a hydrophilic surface.
27211647	6	17	theme	hydrophobic	942:952	arg1	droplet					958:964	a hydrophobic PSE droplet	940:964	a hydrophobic PSE droplet	940:964	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	3	18	theme	PSE/γ-CD	419:426	arg1	particles					428:436	the PSE/γ-CD particles	415:436	the PSE/γ-CD particles	415:436	Cryogenic scanning electron microscopy and contact angle measurements showed that the PSE/γ-CD particles formed a capsule-like structure with a hydrophilic surface.
27211647	3	19	theme	electron	352:359	arg1	microscopy					361:370	Cryogenic scanning electron microscopy	333:370	Cryogenic scanning electron microscopy	333:370	Cryogenic scanning electron microscopy and contact angle measurements showed that the PSE/γ-CD particles formed a capsule-like structure with a hydrophilic surface.
27211647	1	20	theme	undesirable	249:259	arg1	flavours					261:268	undesirable flavours	249:268	undesirable flavours	249:268	The composite material formed by phytosterol ester (PSE) and γ-cyclodextrin (γ-CD) disperses readily in water and has been used to mask undesirable flavours.
27211647	4	21	with	complex	651:657	arg1	γ-CD					664:667	γ-CD	664:667	γ-CD	664:667	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	5	22	theme	differential	784:795	arg1	calorimetry					806:816	differential scanning calorimetry	784:816	differential scanning calorimetry	784:816	The structure of the PSE/γ-CD inclusion complex was similar to that of ChO/γ-CD, based on differential scanning calorimetry and powder X-ray diffractometry results.
27211647	0	23	theme	capsule-like	8:19	arg1	structure					21:29	A novel capsule-like structure	0:29	A novel capsule-like structure of micro-sized particles	0:54	A novel capsule-like structure of micro-sized particles formed by phytosterol ester and γ-cyclodextrin in water.
27211647	5	24	theme	scanning	797:804	arg1	calorimetry					806:816	differential scanning calorimetry	784:816	differential scanning calorimetry	784:816	The structure of the PSE/γ-CD inclusion complex was similar to that of ChO/γ-CD, based on differential scanning calorimetry and powder X-ray diffractometry results.
27211647	0	25	theme	micro-sized	34:44	arg1	particles					46:54	micro-sized particles	34:54	micro-sized particles	34:54	A novel capsule-like structure of micro-sized particles formed by phytosterol ester and γ-cyclodextrin in water.
27211647	6	26	theme	structure	922:930	arg1	wherein					932:938	a capsule-like structure wherein	907:938	a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex	907:1042	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	3	27	with	structure	460:468	arg1	surface					489:495	a hydrophilic surface	475:495	a hydrophilic surface	475:495	Cryogenic scanning electron microscopy and contact angle measurements showed that the PSE/γ-CD particles formed a capsule-like structure with a hydrophilic surface.
27211647	1	28	used	used	236:239	arg2	material					127:134	The composite material	113:134	The composite material formed by phytosterol ester (PSE) and γ-cyclodextrin (γ-CD)	113:194	The composite material formed by phytosterol ester (PSE) and γ-cyclodextrin (γ-CD) disperses readily in water and has been used to mask undesirable flavours.
27211647	6	29	theme	inclusion	1026:1034	arg1	complex					1036:1042	the hydrophilic PSE/γ-CD inclusion complex	1001:1042	the hydrophilic PSE/γ-CD inclusion complex	1001:1042	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	5	30	theme	calorimetry	806:816	arg1	results					850:856	differential scanning calorimetry and powder X-ray diffractometry results	784:856	differential scanning calorimetry and powder X-ray diffractometry results	784:856	The structure of the PSE/γ-CD inclusion complex was similar to that of ChO/γ-CD, based on differential scanning calorimetry and powder X-ray diffractometry results.
27211647	5	31	theme	powder	822:827	arg1	diffractometry					835:848	powder X-ray diffractometry	822:848	powder X-ray diffractometry	822:848	The structure of the PSE/γ-CD inclusion complex was similar to that of ChO/γ-CD, based on differential scanning calorimetry and powder X-ray diffractometry results.
27211647	6	32	theme	capsule-like	909:920	arg1	wherein					932:938	a capsule-like structure wherein	907:938	a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex	907:1042	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	3	33	theme	capsule-like	447:458	arg1	structure					460:468	a capsule-like structure	445:468	a capsule-like structure with a hydrophilic surface	445:495	Cryogenic scanning electron microscopy and contact angle measurements showed that the PSE/γ-CD particles formed a capsule-like structure with a hydrophilic surface.
27211647	3	34	theme	angle	384:388	arg1	measurements					390:401	contact angle measurements	376:401	contact angle measurements	376:401	Cryogenic scanning electron microscopy and contact angle measurements showed that the PSE/γ-CD particles formed a capsule-like structure with a hydrophilic surface.
27211647	4	35	theme	cholesteryl	529:539	arg1	ChO					549:551	ChO	549:551	ChO	549:551	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	4	35	theme	cholesteryl	529:539	arg1	one					555:557	one	555:557	one	555:557	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	4	35	theme	cholesteryl	529:539	arg1	oleate					541:546	cholesteryl oleate	529:546	cholesteryl oleate (ChO)	529:552	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	4	35	theme	cholesteryl	529:539	arg1	components					566:575	the components	562:575	the components of PSE	562:582	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	2	36	theme	PSE/γ-CD	314:321	arg1	particle					323:330	the PSE/γ-CD particle	310:330	the PSE/γ-CD particle	310:330	This paper elucidates the structure of the PSE/γ-CD particle.
27211647	0	37	theme	particles	46:54	arg1	structure					21:29	A novel capsule-like structure	0:29	A novel capsule-like structure of micro-sized particles	0:54	A novel capsule-like structure of micro-sized particles formed by phytosterol ester and γ-cyclodextrin in water.
27211647	6	38	theme	PSE/γ-CD	885:892	arg1	particle					894:901	the PSE/γ-CD particle	881:901	the PSE/γ-CD particle	881:901	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	6	39	dep	wherein	932:938	arg1	surrounded					969:978	surrounded	969:978	is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex	966:1042	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	1	40	theme	composite	117:125	arg1	material					127:134	The composite material	113:134	The composite material formed by phytosterol ester (PSE) and γ-cyclodextrin (γ-CD)	113:194	The composite material formed by phytosterol ester (PSE) and γ-cyclodextrin (γ-CD) disperses readily in water and has been used to mask undesirable flavours.
27211647	6	41	contain	has	903:905	arg1	particle					894:901	the PSE/γ-CD particle	881:901	the PSE/γ-CD particle	881:901	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	6	41	contain	has	903:905	arg2	wherein					932:938	a capsule-like structure wherein	907:938	a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex	907:1042	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	3	42	theme	hydrophilic	477:487	arg1	surface					489:495	a hydrophilic surface	475:495	a hydrophilic surface	475:495	Cryogenic scanning electron microscopy and contact angle measurements showed that the PSE/γ-CD particles formed a capsule-like structure with a hydrophilic surface.
27211647	6	43	theme	outer	986:990	arg1	layer					992:996	an outer layer	983:996	an outer layer of the hydrophilic PSE/γ-CD inclusion complex	983:1042	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	0	44	theme	phytosterol	66:76	arg1	ester					78:82	phytosterol ester	66:82	phytosterol ester	66:82	A novel capsule-like structure of micro-sized particles formed by phytosterol ester and γ-cyclodextrin in water.
27211647	4	45	theme	hydrophilic	610:620	arg1	complex					651:657	a hydrophilic and stoichiometric inclusion complex	608:657	a hydrophilic and stoichiometric inclusion complex with γ-CD	608:667	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	6	46	theme	PSE/γ-CD	1017:1024	arg1	complex					1036:1042	the hydrophilic PSE/γ-CD inclusion complex	1001:1042	the hydrophilic PSE/γ-CD inclusion complex	1001:1042	Thus, we propose that the PSE/γ-CD particle has a capsule-like structure wherein a hydrophobic PSE droplet is surrounded by an outer layer of the hydrophilic PSE/γ-CD inclusion complex.
27211647	5	47	theme	X-ray	829:833	arg1	diffractometry					835:848	powder X-ray diffractometry	822:848	powder X-ray diffractometry	822:848	The structure of the PSE/γ-CD inclusion complex was similar to that of ChO/γ-CD, based on differential scanning calorimetry and powder X-ray diffractometry results.
27211647	5	48	theme	inclusion	724:732	arg1	complex					734:740	the PSE/γ-CD inclusion complex	711:740	the PSE/γ-CD inclusion complex	711:740	The structure of the PSE/γ-CD inclusion complex was similar to that of ChO/γ-CD, based on differential scanning calorimetry and powder X-ray diffractometry results.
27211647	4	49	theme	phase-solubility	500:515	arg1	study					517:521	A phase-solubility study	498:521	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE,	498:583	A phase-solubility study using cholesteryl oleate (ChO), one of the components of PSE, showed that ChO formed a hydrophilic and stoichiometric inclusion complex with γ-CD at a molar ratio of 2:5.
27211647	1	50	theme	phytosterol	146:156	arg1	PSE					165:167	PSE	165:167	PSE	165:167	The composite material formed by phytosterol ester (PSE) and γ-cyclodextrin (γ-CD) disperses readily in water and has been used to mask undesirable flavours.
27211647	1	50	theme	phytosterol	146:156	arg1	ester					158:162	phytosterol ester	146:162	phytosterol ester (PSE)	146:168	The composite material formed by phytosterol ester (PSE) and γ-cyclodextrin (γ-CD) disperses readily in water and has been used to mask undesirable flavours.
25597618	8	0	theme	release	1216:1222	arg1	result					1202:1207	a result	1200:1207	a result of the release of water molecule from the frozen structure	1200:1266	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	8	0	theme	release	1216:1222	arg1	structure					1155:1163	more porous structure	1143:1163	more porous structure	1143:1163	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	8	1	from	structure	1258:1266	arg1	result					1202:1207	a result	1200:1207	a result of the release of water molecule from the frozen structure	1200:1266	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	8	1	from	structure	1258:1266	arg1	release					1216:1222	the release	1212:1222	the release of water molecule from the frozen structure	1212:1266	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	8	1	from	structure	1258:1266	arg1	structure					1155:1163	more porous structure	1143:1163	more porous structure	1143:1163	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	1	2	theme	disintegrating	171:184	arg1	films					186:190	disintegrating films	171:190	disintegrating films	171:190	Freeze drying technology has not been maximized and reported in manufacturing orally disintegrating films.
25597618	7	3	theme	starch	947:952	arg1	starch					947:952	starch	947:952	starch	947:952	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	7	3	theme	starch	947:952	arg1	carbopol					934:941	carbopol	934:941	carbopol	934:941	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	7	3	theme	starch	947:952	arg1	amount					924:929	higher amount	917:929	higher amount of carbopol and starch	917:952	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	6	4	theme	disintegration	878:891	arg1	time					893:896	disintegration time	878:896	disintegration time	878:896	Moreover, films prepared using freeze-dried methods were thicker and had faster disintegration time.
25597618	7	5	theme	PEG	1032:1034	arg1	content					1040:1046	higher PEG 400 content	1025:1046	higher PEG 400 content	1025:1046	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	3	6	theme	polyethylene	530:541	arg1	factors					470:476	three factors	464:476	three factors	464:476	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	3	6	theme	polyethylene	530:541	arg1	glycol					543:548	polyethylene glycol 400	530:552	polyethylene glycol 400	530:552	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	8	7	theme	water	1227:1231	arg1	molecule					1233:1240	water molecule	1227:1240	water molecule	1227:1240	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	0	8	from	Application	0:10	arg1	manufacturing					43:55	manufacturing	43:55	manufacturing	43:55	Application of freeze-drying technology in manufacturing orally disintegrating films.
25597618	8	9	theme	freeze-dried	1120:1131	arg1	films					1133:1137	the freeze-dried films	1116:1137	the freeze-dried films	1116:1137	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	3	10	theme	tensile	561:567	arg1	strength					569:576	tensile strength	561:576	tensile strength	561:576	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	3	11	theme	disintegration	582:595	arg1	time					597:600	disintegration time	582:600	disintegration time	582:600	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	4	12	theme	Heat-dried	615:624	arg1	films					626:630	Heat-dried films	615:630	Heat-dried films	615:630	Heat-dried films had higher tensile strength than films prepared using freeze-dried method.
25597618	7	13	theme	better	1055:1060	arg1	flexibility					1062:1072	better flexibility	1055:1072	better flexibility	1055:1072	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	10	14	theme	51.04	1448:1452	arg1	f2					1442:1443	f2	1442:1443	f2 of 51.04 and 65.98	1442:1462	The dissolution profiles of freeze-dried and heat-dried films were similar to Viagra® with f2 of 51.04 and 65.98, respectively.
25597618	2	15	theme	disintegrating	379:392	arg1	film					394:397	heat-dried orally disintegrating film	361:397	heat-dried orally disintegrating film	361:397	The aim of this study was to explore the freeze drying technology in the formulation of sildenafil orally disintegrating films and compare the physical properties with heat-dried orally disintegrating film.
25597618	7	16	theme	higher	917:922	arg1	starch					947:952	starch	947:952	starch	947:952	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	7	16	theme	higher	917:922	arg1	carbopol					934:941	carbopol	934:941	carbopol	934:941	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	7	16	theme	higher	917:922	arg1	amount					924:929	higher amount	917:929	higher amount of carbopol and starch	917:952	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	3	17	dep	strength	569:576	arg1	the					557:559	the	557:559	the	557:559	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	5	18	theme	heat-dried	780:789	arg1	films					791:795	heat-dried films	780:795	heat-dried films	780:795	For folding endurance, freeze-dried films showed improved endurance than heat-dried films.
25597618	2	19	theme	heat-dried	361:370	arg1	film					394:397	heat-dried orally disintegrating film	361:397	heat-dried orally disintegrating film	361:397	The aim of this study was to explore the freeze drying technology in the formulation of sildenafil orally disintegrating films and compare the physical properties with heat-dried orally disintegrating film.
25597618	10	20	theme	dissolution	1355:1365	arg1	similar					1418:1424	similar	1418:1424	similar	1418:1424	The dissolution profiles of freeze-dried and heat-dried films were similar to Viagra® with f2 of 51.04 and 65.98, respectively.
25597618	10	20	theme	dissolution	1355:1365	arg1	profiles					1367:1374	The dissolution profiles	1351:1374	The dissolution profiles of freeze-dried and heat-dried films	1351:1411	The dissolution profiles of freeze-dried and heat-dried films were similar to Viagra® with f2 of 51.04 and 65.98, respectively.
25597618	7	21	theme	higher	1025:1030	arg1	content					1040:1046	higher PEG 400 content	1025:1046	higher PEG 400 content	1025:1046	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	1	22	theme	Freeze	86:91	arg1	technology					100:109	Freeze drying technology	86:109	Freeze drying technology	86:109	Freeze drying technology has not been maximized and reported in manufacturing orally disintegrating films.
25597618	10	23	theme	freeze-dried	1379:1390	arg1	films					1407:1411	freeze-dried and heat-dried films	1379:1411	freeze-dried and heat-dried films	1379:1411	The dissolution profiles of freeze-dried and heat-dried films were similar to Viagra® with f2 of 51.04 and 65.98, respectively.
25597618	4	24	theme	freeze-dried	686:697	arg1	method					699:704	freeze-dried method	686:704	freeze-dried method	686:704	Heat-dried films had higher tensile strength than films prepared using freeze-dried method.
25597618	8	25	theme	molecule	1233:1240	arg1	release					1216:1222	the release	1212:1222	the release of water molecule from the frozen structure	1212:1266	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	8	26	theme	Scanning	1075:1082	arg1	microscopy					1093:1102	Scanning electron microscopy	1075:1102	Scanning electron microscopy	1075:1102	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	1	27	theme	drying	93:98	arg1	technology					100:109	Freeze drying technology	86:109	Freeze drying technology	86:109	Freeze drying technology has not been maximized and reported in manufacturing orally disintegrating films.
25597618	3	28	theme	factors	470:476	arg1	effects					453:459	the effects	449:459	the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film	449:612	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	8	29	theme	frozen	1251:1256	arg1	structure					1258:1266	the frozen structure	1247:1266	the frozen structure	1247:1266	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	3	30	theme	Central	400:406	arg1	design					418:423	Central composite design	400:423	Central composite design	400:423	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	8	31	theme	porous	1148:1153	arg1	structure					1155:1163	more porous structure	1143:1163	more porous structure	1143:1163	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	8	31	theme	porous	1148:1153	arg1	result					1202:1207	a result	1200:1207	a result of the release of water molecule from the frozen structure	1200:1266	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	6	32	theme	freeze-dried	829:840	arg1	methods					842:848	freeze-dried methods	829:848	freeze-dried methods	829:848	Moreover, films prepared using freeze-dried methods were thicker and had faster disintegration time.
25597618	5	33	theme	folding	711:717	arg1	endurance					719:727	folding endurance	711:727	folding endurance	711:727	For folding endurance, freeze-dried films showed improved endurance than heat-dried films.
25597618	8	34	theme	heat-dried	1181:1190	arg1	film					1192:1195	the heat-dried film	1177:1195	the heat-dried film	1177:1195	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	8	35	theme	more	1143:1146	arg1	structure					1155:1163	more porous structure	1143:1163	more porous structure	1143:1163	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	8	35	theme	more	1143:1146	arg1	result					1202:1207	a result	1200:1207	a result of the release of water molecule from the frozen structure	1200:1266	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	0	36	theme	technology	29:38	arg1	Application					0:10	Application	0:10	Application of freeze-drying technology in manufacturing	0:55	Application of freeze-drying technology in manufacturing orally disintegrating films.
25597618	6	37	dep	faster	871:876	arg1	time					893:896	disintegration time	878:896	disintegration time	878:896	Moreover, films prepared using freeze-dried methods were thicker and had faster disintegration time.
25597618	5	38	theme	freeze-dried	730:741	arg1	films					743:747	freeze-dried films	730:747	freeze-dried films	730:747	For folding endurance, freeze-dried films showed improved endurance than heat-dried films.
25597618	8	39	theme	electron	1084:1091	arg1	microscopy					1093:1102	Scanning electron microscopy	1075:1102	Scanning electron microscopy	1075:1102	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	10	40	theme	65.98	1458:1462	arg1	f2					1442:1443	f2	1442:1443	f2 of 51.04 and 65.98	1442:1462	The dissolution profiles of freeze-dried and heat-dried films were similar to Viagra® with f2 of 51.04 and 65.98, respectively.
25597618	0	41	theme	freeze-drying	15:27	arg1	technology					29:38	freeze-drying technology	15:38	freeze-drying technology	15:38	Application of freeze-drying technology in manufacturing orally disintegrating films.
25597618	2	42	theme	sildenafil	281:290	arg1	films					314:318	sildenafil orally disintegrating films	281:318	sildenafil orally disintegrating films	281:318	The aim of this study was to explore the freeze drying technology in the formulation of sildenafil orally disintegrating films and compare the physical properties with heat-dried orally disintegrating film.
25597618	2	43	with	properties	345:354	arg1	film					394:397	heat-dried orally disintegrating film	361:397	heat-dried orally disintegrating film	361:397	The aim of this study was to explore the freeze drying technology in the formulation of sildenafil orally disintegrating films and compare the physical properties with heat-dried orally disintegrating film.
25597618	2	44	theme	films	314:318	arg1	formulation					266:276	the formulation	262:276	the formulation of sildenafil orally disintegrating films	262:318	The aim of this study was to explore the freeze drying technology in the formulation of sildenafil orally disintegrating films and compare the physical properties with heat-dried orally disintegrating film.
25597618	7	45	with	formulations	1007:1018	arg1	content					1040:1046	higher PEG 400 content	1025:1046	higher PEG 400 content	1025:1046	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	2	46	theme	drying	241:246	arg1	technology					248:257	the freeze drying technology	230:257	the freeze drying technology	230:257	The aim of this study was to explore the freeze drying technology in the formulation of sildenafil orally disintegrating films and compare the physical properties with heat-dried orally disintegrating film.
25597618	10	47	with	similar	1418:1424	arg1	f2					1442:1443	f2	1442:1443	f2 of 51.04 and 65.98	1442:1462	The dissolution profiles of freeze-dried and heat-dried films were similar to Viagra® with f2 of 51.04 and 65.98, respectively.
25597618	8	48	theme	drying	1300:1305	arg1	process					1307:1313	drying process	1300:1313	drying process	1300:1313	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	5	49	theme	improved	756:763	arg1	endurance					765:773	improved endurance	756:773	improved endurance than heat-dried films	756:795	For folding endurance, freeze-dried films showed improved endurance than heat-dried films.
25597618	2	50	theme	freeze	234:239	arg1	technology					248:257	the freeze drying technology	230:257	the freeze drying technology	230:257	The aim of this study was to explore the freeze drying technology in the formulation of sildenafil orally disintegrating films and compare the physical properties with heat-dried orally disintegrating film.
25597618	0	51	theme	disintegrating	64:77	arg1	films					79:83	disintegrating films	64:83	disintegrating films	64:83	Application of freeze-drying technology in manufacturing orally disintegrating films.
25597618	8	52	from	result	1202:1207	arg1	structure					1258:1266	the frozen structure	1247:1266	the frozen structure	1247:1266	Scanning electron microscopy showed that the freeze-dried films had more porous structure compared to the heat-dried film as a result of the release of water molecule from the frozen structure when it was subjected to freeze drying process.
25597618	3	53	theme	wheat	513:517	arg1	factors					470:476	three factors	464:476	three factors	464:476	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	3	53	theme	wheat	513:517	arg1	starch					519:524	wheat starch	513:524	wheat starch	513:524	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	3	54	used	used	429:432	arg2	design					418:423	Central composite design	400:423	Central composite design	400:423	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	7	55	with	Formulations	899:910	arg1	starch					947:952	starch	947:952	starch	947:952	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	7	55	with	Formulations	899:910	arg1	carbopol					934:941	carbopol	934:941	carbopol	934:941	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	7	55	with	Formulations	899:910	arg1	amount					924:929	higher amount	917:929	higher amount of carbopol and starch	917:952	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	3	56	theme	film	609:612	arg1	strength					569:576	tensile strength	561:576	tensile strength	561:576	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	3	56	theme	film	609:612	arg1	time					597:600	disintegration time	582:600	disintegration time	582:600	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	10	57	theme	films	1407:1411	arg1	similar					1418:1424	similar	1418:1424	similar	1418:1424	The dissolution profiles of freeze-dried and heat-dried films were similar to Viagra® with f2 of 51.04 and 65.98, respectively.
25597618	10	57	theme	films	1407:1411	arg1	profiles					1367:1374	The dissolution profiles	1351:1374	The dissolution profiles of freeze-dried and heat-dried films	1351:1411	The dissolution profiles of freeze-dried and heat-dried films were similar to Viagra® with f2 of 51.04 and 65.98, respectively.
25597618	7	58	theme	tensile	968:974	arg1	strength					976:983	higher tensile strength	961:983	higher tensile strength	961:983	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	4	59	contain	had	632:634	arg2	strength					651:658	higher tensile strength	636:658	higher tensile strength	636:658	Heat-dried films had higher tensile strength than films prepared using freeze-dried method.
25597618	4	59	contain	had	632:634	arg1	films					626:630	Heat-dried films	615:630	Heat-dried films	615:630	Heat-dried films had higher tensile strength than films prepared using freeze-dried method.
25597618	9	60	theme	sildenafil	1320:1329	arg1	palatable					1340:1348	palatable	1340:1348	palatable	1340:1348	The sildenafil film was palatable.
25597618	9	60	theme	sildenafil	1320:1329	arg1	film					1331:1334	The sildenafil film	1316:1334	The sildenafil film	1316:1334	The sildenafil film was palatable.
25597618	10	61	theme	heat-dried	1396:1405	arg1	films					1407:1411	freeze-dried and heat-dried films	1379:1411	freeze-dried and heat-dried films	1379:1411	The dissolution profiles of freeze-dried and heat-dried films were similar to Viagra® with f2 of 51.04 and 65.98, respectively.
25597618	3	62	theme	carbopol	503:510	arg1	factors					470:476	three factors	464:476	three factors	464:476	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	3	62	theme	carbopol	503:510	arg1	glycol					543:548	polyethylene glycol 400	530:552	polyethylene glycol 400	530:552	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	3	62	theme	carbopol	503:510	arg1	starch					519:524	wheat starch	513:524	wheat starch	513:524	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	3	62	theme	carbopol	503:510	arg1	concentration					486:498	concentration	486:498	concentration of carbopol	486:510	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	2	63	theme	study	209:213	arg1	aim					197:199	The aim	193:199	The aim of this study	193:213	The aim of this study was to explore the freeze drying technology in the formulation of sildenafil orally disintegrating films and compare the physical properties with heat-dried orally disintegrating film.
25597618	2	64	theme	disintegrating	299:312	arg1	films					314:318	sildenafil orally disintegrating films	281:318	sildenafil orally disintegrating films	281:318	The aim of this study was to explore the freeze drying technology in the formulation of sildenafil orally disintegrating films and compare the physical properties with heat-dried orally disintegrating film.
25597618	3	65	from	effects	453:459	arg1	strength					569:576	tensile strength	561:576	tensile strength	561:576	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	3	65	from	effects	453:459	arg1	time					597:600	disintegration time	582:600	disintegration time	582:600	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	2	66	theme	physical	336:343	arg1	properties					345:354	the physical properties	332:354	the physical properties with heat-dried orally disintegrating film	332:397	The aim of this study was to explore the freeze drying technology in the formulation of sildenafil orally disintegrating films and compare the physical properties with heat-dried orally disintegrating film.
25597618	7	67	theme	carbopol	934:941	arg1	starch					947:952	starch	947:952	starch	947:952	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	7	67	theme	carbopol	934:941	arg1	carbopol					934:941	carbopol	934:941	carbopol	934:941	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	7	67	theme	carbopol	934:941	arg1	amount					924:929	higher amount	917:929	higher amount of carbopol and starch	917:952	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	7	68	theme	higher	961:966	arg1	strength					976:983	higher tensile strength	961:983	higher tensile strength	961:983	Formulations with higher amount of carbopol and starch showed higher tensile strength and thickness whereas formulations with higher PEG 400 content showed better flexibility.
25597618	4	69	theme	tensile	643:649	arg1	strength					651:658	higher tensile strength	636:658	higher tensile strength	636:658	Heat-dried films had higher tensile strength than films prepared using freeze-dried method.
25597618	3	70	theme	composite	408:416	arg1	design					418:423	Central composite design	400:423	Central composite design	400:423	Central composite design was used to investigate the effects of three factors, namely concentration of carbopol, wheat starch and polyethylene glycol 400 on the tensile strength and disintegration time of the film.
25597618	4	71	theme	higher	636:641	arg1	strength					651:658	higher tensile strength	636:658	higher tensile strength	636:658	Heat-dried films had higher tensile strength than films prepared using freeze-dried method.
24719381	3	0	theme	negative	718:725	arg1	shift					727:731	a negative shift	716:731	a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes	716:811	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	0	1	dep	units	184:188	arg1	both					164:167	both	164:167	both	164:167	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.
24719381	0	1	dep	units	184:188	arg1	host					179:182	host	179:182	host	179:182	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.
24719381	1	2	theme	ion-recognition	217:231	arg1	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide					249:300	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide	249:300	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide	249:300	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	2	theme	ion-recognition	217:231	arg1	poly					244:247	Novel dual molecular- and ion-recognition responsive poly	191:247	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units	191:390	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	2	theme	ion-recognition	217:231	arg1	C4					310:311	PNB12 C4	304:311	PNB12 C4	304:311	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	4	3	theme	competitive	896:906	arg1	actions					921:927	competitive complexation actions	896:927	competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+)	896:987	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	3	4	theme	copolymer	589:597	arg1	LCST					571:574	LCST	571:574	LCST	571:574	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	3	4	theme	copolymer	589:597	arg1	temperature					558:568	the lower critical solution temperature	530:568	the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value	530:624	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	3	5	theme	host-guest	792:801	arg1	complexes					803:811	2:1 "sandwich" B12C4/Na(+) host-guest complexes	765:811	2:1 "sandwich" B12C4/Na(+) host-guest complexes	765:811	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	4	6	theme	LCST	1072:1075	arg1	values					1077:1082	the final LCST values	1062:1082	the final LCST values of PNB12 C4 copolymer	1062:1104	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	0	7	with	copolymers	128:137	arg1	benzo-12-crown-4					144:159	benzo-12-crown-4	144:159	benzo-12-crown-4	144:159	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.
24719381	2	8	theme	selective	436:444	arg1	sensitivities					446:458	highly selective sensitivities	429:458	highly selective sensitivities	429:458	The copolymers exhibit highly selective sensitivities toward γ-cyclodextrin (γ-CD) and Na(+) .
24719381	5	9	theme	various	1235:1241	arg1	applications					1243:1254	various applications	1235:1254	various applications	1235:1254	The results provide valuable guidance for designing and applying PNB12 C4 -based smart materials in various applications.
24719381	3	10	theme	complexes	803:811	arg1	formation					752:760	the formation	748:760	the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes	748:811	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	0	11	dep	both	164:167	arg1	guest					169:173	guest	169:173	guest	169:173	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.
24719381	1	12	theme	responsive	233:242	arg1	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide					249:300	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide	249:300	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide	249:300	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	12	theme	responsive	233:242	arg1	poly					244:247	Novel dual molecular- and ion-recognition responsive poly	191:247	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units	191:390	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	12	theme	responsive	233:242	arg1	C4					310:311	PNB12 C4	304:311	PNB12 C4	304:311	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	4	13	theme	complexation	908:919	arg1	actions					921:927	competitive complexation actions	896:927	competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+)	896:987	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	3	14	theme	C4	586:587	arg1	copolymer					589:597	PNB12 C4 copolymer	580:597	PNB12 C4 copolymer	580:597	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	1	15	theme	poly	244:247	arg1	copolymers					322:331	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers	191:331	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units	191:390	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	0	16	theme	molecular-/ion-recognition	12:37	arg1	characteristics					50:64	Competitive molecular-/ion-recognition responsive characteristics	0:64	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.	0:189	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.
24719381	4	17	theme	B12C4	932:936	arg1	actions					921:927	competitive complexation actions	896:927	competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+)	896:987	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	0	18	theme	Competitive	0:10	arg1	characteristics					50:64	Competitive molecular-/ion-recognition responsive characteristics	0:64	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.	0:189	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.
24719381	4	19	theme	complexation	832:843	arg1	order					845:849	the complexation order	828:849	the complexation order	828:849	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	3	20	theme	higher	613:618	arg1	value					620:624	a higher value	611:624	a higher value	611:624	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	5	21	theme	-based	1209:1214	arg1	materials					1222:1230	PNB12 C4 -based smart materials	1200:1230	PNB12 C4 -based smart materials	1200:1230	The results provide valuable guidance for designing and applying PNB12 C4 -based smart materials in various applications.
24719381	4	22	theme	2:2:1	1014:1018	arg1	γ-CD/B12C4/Na					1020:1032	equilibrium 2:2:1 γ-CD/B12C4/Na	1002:1032	equilibrium 2:2:1 γ-CD/B12C4/Na	1002:1032	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	4	23	theme	same	1123:1126	arg1	level					1128:1132	almost the same level	1112:1132	almost the same level	1112:1132	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	5	24	theme	smart	1216:1220	arg1	materials					1222:1230	PNB12 C4 -based smart materials	1200:1230	PNB12 C4 -based smart materials	1200:1230	The results provide valuable guidance for designing and applying PNB12 C4 -based smart materials in various applications.
24719381	0	25	theme	responsive	39:48	arg1	characteristics					50:64	Competitive molecular-/ion-recognition responsive characteristics	0:64	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.	0:189	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.
24719381	1	26	theme	guest	371:375	arg1	units					386:390	both guest and host units	366:390	units	386:390	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	3	27	theme	PNB12	580:584	arg1	copolymer					589:597	PNB12 C4 copolymer	580:597	PNB12 C4 copolymer	580:597	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	3	28	theme	complexes	686:694	arg1	formation					637:645	the formation	633:645	the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes	633:694	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	4	29	theme	composite	1037:1045	arg1	complexes					1047:1055	equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes	1002:1055	equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes	1002:1055	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	0	30	theme	poly	69:72	arg1	copolymers					128:137	poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers	69:137	poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4	69:159	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.
24719381	3	31	from	shift	727:731	arg1	LCST					736:739	LCST	736:739	LCST	736:739	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	4	32	theme	guest	946:950	arg1	units					961:965	both guest and host units	941:965	units	961:965	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	1	33	theme	host	381:384	arg1	units					386:390	both guest and host units	366:390	units	386:390	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	4	34	theme	PNB12	885:889	arg1	C4					891:892	PNB12 C4	885:892	PNB12 C4	885:892	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	3	35	theme	host-guest	665:674	arg1	complexes					686:694	1:1 γ-CD/B12C4 host-guest inclusion complexes	650:694	1:1 γ-CD/B12C4 host-guest inclusion complexes	650:694	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	4	36	theme	C4	1093:1094	arg1	copolymer					1096:1104	PNB12 C4 copolymer	1087:1104	PNB12 C4 copolymer	1087:1104	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	3	37	theme	γ-CD	517:520	arg1	presence					505:512	The presence	501:512	The presence of γ-CD	501:520	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	3	38	theme	sandwich	770:777	arg1	B12C4/Na					780:787	2:1 "sandwich" B12C4/Na	765:787	2:1 "sandwich" B12C4/Na	765:787	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	3	39	theme	inclusion	676:684	arg1	complexes					686:694	1:1 γ-CD/B12C4 host-guest inclusion complexes	650:694	1:1 γ-CD/B12C4 host-guest inclusion complexes	650:694	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	4	40	theme	PNB12	1087:1091	arg1	copolymer					1096:1104	PNB12 C4 copolymer	1087:1104	PNB12 C4 copolymer	1087:1104	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	3	41	theme	solution	549:556	arg1	LCST					571:574	LCST	571:574	LCST	571:574	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	3	41	theme	solution	549:556	arg1	temperature					558:568	the lower critical solution temperature	530:568	the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value	530:624	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	4	42	theme	host	956:959	arg1	units					961:965	both guest and host units	941:965	units	961:965	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	3	43	theme	2:1	765:767	arg1	B12C4/Na					780:787	2:1 "sandwich" B12C4/Na	765:787	2:1 "sandwich" B12C4/Na	765:787	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	4	44	theme	equilibrium	1002:1012	arg1	γ-CD/B12C4/Na					1020:1032	equilibrium 2:2:1 γ-CD/B12C4/Na	1002:1032	equilibrium 2:2:1 γ-CD/B12C4/Na	1002:1032	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	3	45	theme	1:1	650:652	arg1	complexes					686:694	1:1 γ-CD/B12C4 host-guest inclusion complexes	650:694	1:1 γ-CD/B12C4 host-guest inclusion complexes	650:694	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	4	46	theme	copolymer	1096:1104	arg1	values					1077:1082	the final LCST values	1062:1082	the final LCST values of PNB12 C4 copolymer	1062:1104	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	3	47	theme	lower	534:538	arg1	LCST					571:574	LCST	571:574	LCST	571:574	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	3	47	theme	lower	534:538	arg1	temperature					558:568	the lower critical solution temperature	530:568	the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value	530:624	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	3	48	theme	γ-CD/B12C4	654:663	arg1	complexes					686:694	1:1 γ-CD/B12C4 host-guest inclusion complexes	650:694	1:1 γ-CD/B12C4 host-guest inclusion complexes	650:694	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	1	49	with	copolymers	322:331	arg1	B12C4					356:360	B12C4	356:360	B12C4	356:360	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	49	with	copolymers	322:331	arg1	benzo-12-crown-4					338:353	benzo-12-crown-4	338:353	benzo-12-crown-4 (B12C4)	338:361	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	50	theme	Novel	191:195	arg1	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide					249:300	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide	249:300	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide	249:300	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	50	theme	Novel	191:195	arg1	poly					244:247	Novel dual molecular- and ion-recognition responsive poly	191:247	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units	191:390	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	50	theme	Novel	191:195	arg1	C4					310:311	PNB12 C4	304:311	PNB12 C4	304:311	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	4	51	theme	γ-CD/B12C4/Na	1020:1032	arg1	complexes					1047:1055	equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes	1002:1055	equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes	1002:1055	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	1	52	theme	linear	315:320	arg1	copolymers					322:331	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers	191:331	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units	191:390	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	0	53	theme	copolymers	128:137	arg1	characteristics					50:64	Competitive molecular-/ion-recognition responsive characteristics	0:64	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.	0:189	Competitive molecular-/ion-recognition responsive characteristics of poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) copolymers with benzo-12-crown-4 as both guest and host units.
24719381	4	54	theme	final	1066:1070	arg1	values					1077:1082	the final LCST values	1062:1082	the final LCST values of PNB12 C4 copolymer	1062:1104	Regardless of the complexation order, when γ-CD and Na(+) coexist with PNB12 C4 , competitive complexation actions of B12C4 as both guest and host units toward γ-CD and Na(+) finally form equilibrium 2:2:1 γ-CD/B12C4/Na(+) composite complexes, and the final LCST values of PNB12 C4 copolymer reach almost the same level.
24719381	1	55	theme	dual	197:200	arg1	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide					249:300	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide	249:300	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide	249:300	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	55	theme	dual	197:200	arg1	poly					244:247	Novel dual molecular- and ion-recognition responsive poly	191:247	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units	191:390	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	55	theme	dual	197:200	arg1	C4					310:311	PNB12 C4	304:311	PNB12 C4	304:311	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	3	56	theme	critical	540:547	arg1	LCST					571:574	LCST	571:574	LCST	571:574	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	3	56	theme	critical	540:547	arg1	temperature					558:568	the lower critical solution temperature	530:568	the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value	530:624	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	3	57	theme	B12C4/Na	780:787	arg1	complexes					803:811	2:1 "sandwich" B12C4/Na(+) host-guest complexes	765:811	2:1 "sandwich" B12C4/Na(+) host-guest complexes	765:811	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
24719381	1	58	theme	molecular-	202:211	arg1	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide					249:300	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide	249:300	N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide	249:300	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	58	theme	molecular-	202:211	arg1	poly					244:247	Novel dual molecular- and ion-recognition responsive poly	191:247	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units	191:390	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	1	58	theme	molecular-	202:211	arg1	C4					310:311	PNB12 C4	304:311	PNB12 C4	304:311	Novel dual molecular- and ion-recognition responsive poly(N-isopropylacrylamide-co-benzo-12-crown-4-acrylamide) (PNB12 C4 ) linear copolymers with benzo-12-crown-4 (B12C4) as both guest and host units are prepared.
24719381	5	59	theme	valuable	1155:1162	arg1	guidance					1164:1171	valuable guidance	1155:1171	valuable guidance for designing and applying PNB12 C4 -based smart materials in various applications	1155:1254	The results provide valuable guidance for designing and applying PNB12 C4 -based smart materials in various applications.
24719381	3	60	theme	"	778:778	arg1	B12C4/Na					780:787	2:1 "sandwich" B12C4/Na	765:787	2:1 "sandwich" B12C4/Na	765:787	The presence of γ-CD induces the lower critical solution temperature (LCST) of PNB12 C4 copolymer to shift to a higher value due to the formation of 1:1 γ-CD/B12C4 host-guest inclusion complexes, while Na(+) causes a negative shift in LCST due to the formation of 2:1 "sandwich" B12C4/Na(+) host-guest complexes.
26050895	0	0	theme	pectin	74:79	arg1	yield					45:49	yield	45:49	yield	45:49	Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid.
26050895	0	0	theme	pectin	74:79	arg1	content					63:69	protein content	55:69	protein content	55:69	Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid.
26050895	7	1	theme	different	1120:1128	arg1	property					1142:1149	different emulsifying property	1120:1149	different emulsifying property	1120:1149	Therefore, it was inferred that the extraction conditions could influence yield and protein content, resulting in different emulsifying property.
26050895	1	2	theme	different	211:219	arg1	conditions					221:230	different conditions	211:230	different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio)	211:336	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	7	3	theme	emulsifying	1130:1140	arg1	property					1142:1149	different emulsifying property	1120:1149	different emulsifying property	1120:1149	Therefore, it was inferred that the extraction conditions could influence yield and protein content, resulting in different emulsifying property.
26050895	0	4	from	effects	9:15	arg1	yield					45:49	yield	45:49	yield	45:49	Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid.
26050895	0	4	from	effects	9:15	arg1	content					63:69	protein content	55:69	protein content	55:69	Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid.
26050895	5	5	theme	chemical	801:808	arg1	composition					810:820	The chemical composition	797:820	The chemical composition of collected samples	797:841	The chemical composition of collected samples was determined.
26050895	6	6	theme	properties	901:910	arg1	study					912:916	emulsifying properties study	889:916	emulsifying properties study	889:916	Moreover, from the results of emulsifying properties study, the extracted pectin from sugar beet pulp could prepare steady oil-in-water emulsions.
26050895	2	7	theme	1.5	434:436	arg1	%					437:437	%	437:437	%	437:437	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	2	8	dep	%	399:399	arg1	to					392:393	to	392:393	to	392:393	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	6	9	theme	sugar	945:949	arg1	pulp					956:959	sugar beet pulp	945:959	sugar beet pulp	945:959	Moreover, from the results of emulsifying properties study, the extracted pectin from sugar beet pulp could prepare steady oil-in-water emulsions.
26050895	6	10	theme	emulsifying	889:899	arg1	study					912:916	emulsifying properties study	889:916	emulsifying properties study	889:916	Moreover, from the results of emulsifying properties study, the extracted pectin from sugar beet pulp could prepare steady oil-in-water emulsions.
26050895	2	11	dep	%	437:437	arg1	to					439:440	to	439:440	to	439:440	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	3	12	theme	protein	582:588	arg1	content					590:596	the protein content	578:596	the protein content	578:596	All independent variables significantly affected the yield, and all variables except liquid to solid ratio significantly affected the protein content.
26050895	4	13	theme	protein	775:781	arg1	variables					750:758	variables	750:758	variables	750:758	The yield increased as decreasing pH of extracting solution, extending time and advancing temperature, and an opposite relationship of effects between variables and content of protein was obtained.
26050895	4	13	theme	protein	775:781	arg1	content					764:770	content	764:770	content of protein	764:781	The yield increased as decreasing pH of extracting solution, extending time and advancing temperature, and an opposite relationship of effects between variables and content of protein was obtained.
26050895	1	14	theme	Box-Behnken	238:248	arg1	design					250:255	Box-Behnken design	238:255	Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio)	238:336	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	0	15	theme	beet	102:105	arg1	pulp					107:110	sugar beet pulp	96:110	sugar beet pulp	96:110	Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid.
26050895	6	16	theme	beet	951:954	arg1	pulp					956:959	sugar beet pulp	945:959	sugar beet pulp	945:959	Moreover, from the results of emulsifying properties study, the extracted pectin from sugar beet pulp could prepare steady oil-in-water emulsions.
26050895	1	17	theme	pectin	146:151	arg1	extraction					132:141	The extraction	128:141	The extraction of pectin from sugar beet pulp by citric acid	128:187	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	0	18	theme	sugar	96:100	arg1	pulp					107:110	sugar beet pulp	96:110	sugar beet pulp	96:110	Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid.
26050895	1	19	theme	solid	325:329	arg1	ratio					331:335	solid ratio	325:335	solid ratio	325:335	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	4	20	theme	effects	734:740	arg1	relationship					718:729	an opposite relationship	706:729	an opposite relationship of effects between variables and content of protein	706:781	The yield increased as decreasing pH of extracting solution, extending time and advancing temperature, and an opposite relationship of effects between variables and content of protein was obtained.
26050895	7	21	theme	protein	1090:1096	arg1	content					1098:1104	protein content	1090:1104	protein content	1090:1104	Therefore, it was inferred that the extraction conditions could influence yield and protein content, resulting in different emulsifying property.
26050895	2	22	theme	protein	421:427	arg1	protein					421:427	protein	421:427	protein	421:427	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	2	22	theme	protein	421:427	arg1	content					410:416	the content	406:416	the content of protein	406:427	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	2	22	theme	protein	421:427	arg1	%					399:399	6.3% to 23.0%	387:399	6.3% to 23.0%	387:399	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	3	23	theme	solid	543:547	arg1	ratio					549:553	solid ratio	543:553	solid ratio	543:553	All independent variables significantly affected the yield, and all variables except liquid to solid ratio significantly affected the protein content.
26050895	6	24	theme	oil-in-water	982:993	arg1	emulsions					995:1003	steady oil-in-water emulsions	975:1003	steady oil-in-water emulsions	975:1003	Moreover, from the results of emulsifying properties study, the extracted pectin from sugar beet pulp could prepare steady oil-in-water emulsions.
26050895	3	25	theme	independent	452:462	arg1	variables					464:472	All independent variables	448:472	All independent variables	448:472	All independent variables significantly affected the yield, and all variables except liquid to solid ratio significantly affected the protein content.
26050895	1	26	theme	sugar	158:162	arg1	pulp					169:172	sugar beet pulp	158:172	sugar beet pulp	158:172	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	0	27	theme	Combined	0:7	arg1	effects					9:15	Combined effects	0:15	Combined effects of independent variables on yield and protein content of pectin	0:79	Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid.
26050895	6	28	from	pulp	956:959	arg1	pectin					933:938	the extracted pectin	919:938	the extracted pectin from sugar beet pulp	919:959	Moreover, from the results of emulsifying properties study, the extracted pectin from sugar beet pulp could prepare steady oil-in-water emulsions.
26050895	6	29	theme	study	912:916	arg1	results					878:884	the results	874:884	the results of emulsifying properties study	874:916	Moreover, from the results of emulsifying properties study, the extracted pectin from sugar beet pulp could prepare steady oil-in-water emulsions.
26050895	1	30	theme	beet	164:167	arg1	pulp					169:172	sugar beet pulp	158:172	sugar beet pulp	158:172	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	0	31	theme	independent	20:30	arg1	variables					32:40	independent variables	20:40	independent variables	20:40	Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid.
26050895	4	32	theme	solution	650:657	arg1	pH					633:634	pH	633:634	pH of extracting solution	633:657	The yield increased as decreasing pH of extracting solution, extending time and advancing temperature, and an opposite relationship of effects between variables and content of protein was obtained.
26050895	2	33	theme	pulp	363:366	arg1	pectin					368:373	sugar beet pulp pectin	352:373	sugar beet pulp pectin	352:373	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	2	34	theme	pectin	368:373	arg1	yield					343:347	The yield	339:347	The yield of sugar beet pulp pectin	339:373	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	2	35	theme	4.5	442:444	arg1	%					437:437	%	437:437	%	437:437	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	4	36	theme	extracting	639:648	arg1	solution					650:657	extracting solution	639:657	extracting solution	639:657	The yield increased as decreasing pH of extracting solution, extending time and advancing temperature, and an opposite relationship of effects between variables and content of protein was obtained.
26050895	2	37	theme	beet	358:361	arg1	pectin					368:373	sugar beet pulp pectin	352:373	sugar beet pulp pectin	352:373	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	1	38	theme	independent	266:276	arg1	time					306:309	time	306:309	time	306:309	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	1	38	theme	independent	266:276	arg1	temperature					293:303	temperature	293:303	temperature	293:303	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	1	38	theme	independent	266:276	arg1	pH					289:290	pH	289:290	pH	289:290	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	1	38	theme	independent	266:276	arg1	variables					278:286	four independent variables	261:286	four independent variables (pH, temperature, time and liquid to solid ratio)	261:336	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	2	39	theme	sugar	352:356	arg1	pectin					368:373	sugar beet pulp pectin	352:373	sugar beet pulp pectin	352:373	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	4	40	theme	opposite	709:716	arg1	relationship					718:729	an opposite relationship	706:729	an opposite relationship of effects between variables and content of protein	706:781	The yield increased as decreasing pH of extracting solution, extending time and advancing temperature, and an opposite relationship of effects between variables and content of protein was obtained.
26050895	1	41	theme	citric	177:182	arg1	acid					184:187	citric acid	177:187	citric acid	177:187	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	0	42	theme	variables	32:40	arg1	effects					9:15	Combined effects	0:15	Combined effects of independent variables on yield and protein content of pectin	0:79	Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid.
26050895	0	43	theme	citric	115:120	arg1	acid					122:125	citric acid	115:125	citric acid	115:125	Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid.
26050895	1	44	dep	variables	278:286	arg1	time					306:309	time	306:309	time	306:309	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	1	44	dep	variables	278:286	arg1	temperature					293:303	temperature	293:303	temperature	293:303	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	1	44	dep	variables	278:286	arg1	pH					289:290	pH	289:290	pH	289:290	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	1	44	dep	variables	278:286	arg1	variables					278:286	four independent variables	261:286	four independent variables (pH, temperature, time and liquid to solid ratio)	261:336	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	1	44	dep	variables	278:286	arg1	liquid					315:320	liquid	315:320	liquid	315:320	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	2	45	theme	%	390:390	arg1	protein					421:427	protein	421:427	protein	421:427	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	2	45	theme	%	390:390	arg1	%					399:399	6.3% to 23.0%	387:399	6.3% to 23.0%	387:399	The yield of sugar beet pulp pectin ranged from 6.3% to 23.0%, and the content of protein from 1.5% to 4.5%.
26050895	1	46	from	pulp	169:172	arg1	extraction					132:141	The extraction	128:141	The extraction of pectin from sugar beet pulp by citric acid	128:187	The extraction of pectin from sugar beet pulp by citric acid was carried out under different conditions using Box-Behnken design for four independent variables (pH, temperature, time and liquid to solid ratio).
26050895	7	47	theme	extraction	1042:1051	arg1	conditions					1053:1062	the extraction conditions	1038:1062	the extraction conditions	1038:1062	Therefore, it was inferred that the extraction conditions could influence yield and protein content, resulting in different emulsifying property.
26050895	5	48	theme	collected	825:833	arg1	samples					835:841	collected samples	825:841	collected samples	825:841	The chemical composition of collected samples was determined.
26050895	5	49	theme	samples	835:841	arg1	composition					810:820	The chemical composition	797:820	The chemical composition of collected samples	797:841	The chemical composition of collected samples was determined.
26050895	6	50	theme	extracted	923:931	arg1	pectin					933:938	the extracted pectin	919:938	the extracted pectin from sugar beet pulp	919:959	Moreover, from the results of emulsifying properties study, the extracted pectin from sugar beet pulp could prepare steady oil-in-water emulsions.
26050895	6	51	theme	steady	975:980	arg1	emulsions					995:1003	steady oil-in-water emulsions	975:1003	steady oil-in-water emulsions	975:1003	Moreover, from the results of emulsifying properties study, the extracted pectin from sugar beet pulp could prepare steady oil-in-water emulsions.
26050895	0	52	theme	protein	55:61	arg1	content					63:69	protein content	55:69	protein content	55:69	Combined effects of independent variables on yield and protein content of pectin extracted from sugar beet pulp by citric acid.
25656863	6	0	theme	energy	876:881	arg1	glucose/kWh					906:916	η=0.116 kg glucose/kWh	895:916	η=0.116 kg glucose/kWh	895:916	Moreover, the highest energy efficiency (η=0.116 kg glucose/kWh) was obtained with NaOH-CM.
25656863	6	0	theme	energy	876:881	arg1	efficiency					883:892	the highest energy efficiency	864:892	the highest energy efficiency (η=0.116 kg glucose/kWh)	864:917	Moreover, the highest energy efficiency (η=0.116 kg glucose/kWh) was obtained with NaOH-CM.
25656863	7	1	theme	bioethanol	1069:1078	arg1	production					1055:1064	the production	1051:1064	the production of bioethanol from SB	1051:1086	Therefore, the combination of dry NaOH and CM appears the most suitable and interesting pretreatment for the production of bioethanol from SB.
25656863	1	2	theme	dry	153:155	arg1	pretreatment					174:185	an innovative dry chemo-mechanical pretreatment	139:185	an innovative dry chemo-mechanical pretreatment using different mechanical stresses	139:221	The aim of this study was the application of an innovative dry chemo-mechanical pretreatment using different mechanical stresses to produce bioethanol from sugarcane bagasse (SB).
25656863	7	3	theme	CM	989:990	arg1	combination					961:971	the combination	957:971	the combination of dry NaOH and CM	957:990	Therefore, the combination of dry NaOH and CM appears the most suitable and interesting pretreatment for the production of bioethanol from SB.
25656863	6	4	theme	highest	868:874	arg1	glucose/kWh					906:916	η=0.116 kg glucose/kWh	895:916	η=0.116 kg glucose/kWh	895:916	Moreover, the highest energy efficiency (η=0.116 kg glucose/kWh) was obtained with NaOH-CM.
25656863	6	4	theme	highest	868:874	arg1	efficiency					883:892	the highest energy efficiency	864:892	the highest energy efficiency (η=0.116 kg glucose/kWh)	864:917	Moreover, the highest energy efficiency (η=0.116 kg glucose/kWh) was obtained with NaOH-CM.
25656863	1	5	theme	chemo-mechanical	157:172	arg1	pretreatment					174:185	an innovative dry chemo-mechanical pretreatment	139:185	an innovative dry chemo-mechanical pretreatment using different mechanical stresses	139:221	The aim of this study was the application of an innovative dry chemo-mechanical pretreatment using different mechanical stresses to produce bioethanol from sugarcane bagasse (SB).
25656863	2	6	theme	bioethanol	368:377	arg1	production					379:388	bioethanol production	368:388	bioethanol production	368:388	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency was also evaluated.
25656863	2	7	theme	milling	298:304	arg1	methods					306:312	different milling methods	288:312	different milling methods	288:312	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency was also evaluated.
25656863	2	8	theme	methods	306:312	arg1	effect					278:283	The effect	274:283	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency	274:410	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency was also evaluated.
25656863	4	9	from	reduction	584:592	arg1	effective					567:575	effective	567:575	effective	567:575	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	2	10	theme	different	288:296	arg1	methods					306:312	different milling methods	288:312	different milling methods	288:312	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency was also evaluated.
25656863	5	11	theme	bioethanol	777:786	arg1	production					788:797	bioethanol production	777:797	bioethanol production	777:797	NaOH pretreatment coupling to BM and VBM was preferred to enhance glucose yields and bioethanol production, while CM consumed less energy compared to BM and VBM.
25656863	1	12	theme	pretreatment	174:185	arg1	application					124:134	the application	120:134	the application of an innovative dry chemo-mechanical pretreatment using different mechanical stresses	120:221	The aim of this study was the application of an innovative dry chemo-mechanical pretreatment using different mechanical stresses to produce bioethanol from sugarcane bagasse (SB).
25656863	4	13	theme	particles	597:605	arg1	reduction					584:592	the reduction	580:592	the reduction of particles size and cellulose crystallinity	580:638	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	4	14	from	effective	567:575	arg1	reduction					584:592	the reduction	580:592	the reduction of particles size and cellulose crystallinity	580:638	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	7	15	theme	interesting	1022:1032	arg1	pretreatment					1034:1045	the most suitable and interesting pretreatment	1000:1045	the most suitable and interesting pretreatment for the production of bioethanol from SB	1000:1086	Therefore, the combination of dry NaOH and CM appears the most suitable and interesting pretreatment for the production of bioethanol from SB.
25656863	1	16	theme	different	193:201	arg1	stresses					214:221	different mechanical stresses	193:221	different mechanical stresses	193:221	The aim of this study was the application of an innovative dry chemo-mechanical pretreatment using different mechanical stresses to produce bioethanol from sugarcane bagasse (SB).
25656863	2	17	theme	physicochemical	317:331	arg1	composition					333:343	physicochemical composition	317:343	physicochemical composition	317:343	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency was also evaluated.
25656863	1	18	theme	mechanical	203:212	arg1	stresses					214:221	different mechanical stresses	193:221	different mechanical stresses	193:221	The aim of this study was the application of an innovative dry chemo-mechanical pretreatment using different mechanical stresses to produce bioethanol from sugarcane bagasse (SB).
25656863	7	19	theme	suitable	1009:1016	arg1	pretreatment					1034:1045	the most suitable and interesting pretreatment	1000:1045	the most suitable and interesting pretreatment for the production of bioethanol from SB	1000:1086	Therefore, the combination of dry NaOH and CM appears the most suitable and interesting pretreatment for the production of bioethanol from SB.
25656863	3	20	theme	materials	478:486	arg1	kg/L					505:508	5 kg/L	503:508	5 kg/L	503:508	SB was pretreated with NaOH and H3PO4 at high materials concentration (5 kg/L).
25656863	3	20	theme	materials	478:486	arg1	concentration					488:500	high materials concentration	473:500	high materials concentration (5 kg/L)	473:509	SB was pretreated with NaOH and H3PO4 at high materials concentration (5 kg/L).
25656863	0	21	theme	chemo-mechanical	12:27	arg1	deconstruction					29:42	chemo-mechanical deconstruction	12:42	chemo-mechanical deconstruction for bioethanol production from sugarcane bagasse	12:91	One-Pot dry chemo-mechanical deconstruction for bioethanol production from sugarcane bagasse.
25656863	1	22	theme	study	110:114	arg1	aim					98:100	The aim	94:100	The aim of this study	94:114	The aim of this study was the application of an innovative dry chemo-mechanical pretreatment using different mechanical stresses to produce bioethanol from sugarcane bagasse (SB).
25656863	0	23	theme	bioethanol	48:57	arg1	production					59:68	bioethanol production	48:68	bioethanol production from sugarcane bagasse	48:91	One-Pot dry chemo-mechanical deconstruction for bioethanol production from sugarcane bagasse.
25656863	5	24	theme	NaOH	692:695	arg1	coupling					710:717	NaOH pretreatment coupling	692:717	NaOH pretreatment coupling to BM and VBM	692:731	NaOH pretreatment coupling to BM and VBM was preferred to enhance glucose yields and bioethanol production, while CM consumed less energy compared to BM and VBM.
25656863	5	25	theme	glucose	758:764	arg1	yields					766:771	glucose yields	758:771	glucose yields	758:771	NaOH pretreatment coupling to BM and VBM was preferred to enhance glucose yields and bioethanol production, while CM consumed less energy compared to BM and VBM.
25656863	5	26	theme	pretreatment	697:708	arg1	coupling					710:717	NaOH pretreatment coupling	692:717	NaOH pretreatment coupling to BM and VBM	692:731	NaOH pretreatment coupling to BM and VBM was preferred to enhance glucose yields and bioethanol production, while CM consumed less energy compared to BM and VBM.
25656863	0	27	from	bagasse	85:91	arg1	production					59:68	bioethanol production	48:68	bioethanol production from sugarcane bagasse	48:91	One-Pot dry chemo-mechanical deconstruction for bioethanol production from sugarcane bagasse.
25656863	3	28	theme	high	473:476	arg1	kg/L					505:508	5 kg/L	503:508	5 kg/L	503:508	SB was pretreated with NaOH and H3PO4 at high materials concentration (5 kg/L).
25656863	3	28	theme	high	473:476	arg1	concentration					488:500	high materials concentration	473:500	high materials concentration (5 kg/L)	473:509	SB was pretreated with NaOH and H3PO4 at high materials concentration (5 kg/L).
25656863	4	29	theme	vibratory	534:542	arg1	VBM					553:555	VBM	553:555	VBM	553:555	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	4	29	theme	vibratory	534:542	arg1	milling					544:550	vibratory milling	534:550	vibratory milling (VBM)	534:556	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	1	30	from	bagasse	260:266	arg1	bioethanol					234:243	bioethanol	234:243	bioethanol from sugarcane bagasse (SB)	234:271	The aim of this study was the application of an innovative dry chemo-mechanical pretreatment using different mechanical stresses to produce bioethanol from sugarcane bagasse (SB).
25656863	7	31	theme	dry	976:978	arg1	NaOH					980:983	dry NaOH	976:983	dry NaOH	976:983	Therefore, the combination of dry NaOH and CM appears the most suitable and interesting pretreatment for the production of bioethanol from SB.
25656863	6	32	theme	η=0.116	895:901	arg1	glucose/kWh					906:916	η=0.116 kg glucose/kWh	895:916	η=0.116 kg glucose/kWh	895:916	Moreover, the highest energy efficiency (η=0.116 kg glucose/kWh) was obtained with NaOH-CM.
25656863	6	32	theme	η=0.116	895:901	arg1	efficiency					883:892	the highest energy efficiency	864:892	the highest energy efficiency (η=0.116 kg glucose/kWh)	864:917	Moreover, the highest energy efficiency (η=0.116 kg glucose/kWh) was obtained with NaOH-CM.
25656863	7	33	theme	NaOH	980:983	arg1	combination					961:971	the combination	957:971	the combination of dry NaOH and CM	957:990	Therefore, the combination of dry NaOH and CM appears the most suitable and interesting pretreatment for the production of bioethanol from SB.
25656863	2	34	theme	enzymatic	346:354	arg1	hydrolysis					356:365	enzymatic hydrolysis	346:365	enzymatic hydrolysis	346:365	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency was also evaluated.
25656863	2	35	from	effect	278:283	arg1	hydrolysis					356:365	enzymatic hydrolysis	346:365	enzymatic hydrolysis	346:365	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency was also evaluated.
25656863	2	35	from	effect	278:283	arg1	production					379:388	bioethanol production	368:388	bioethanol production	368:388	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency was also evaluated.
25656863	2	35	from	effect	278:283	arg1	efficiency					401:410	energy efficiency	394:410	energy efficiency	394:410	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency was also evaluated.
25656863	2	35	from	effect	278:283	arg1	composition					333:343	physicochemical composition	317:343	physicochemical composition	317:343	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency was also evaluated.
25656863	4	36	theme	ball	673:676	arg1	BM					679:680	BM	679:680	BM	679:680	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	4	36	theme	ball	673:676	arg1	milling					683:689	centrifugal (CM) and ball (BM) milling	652:689	milling	683:689	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	1	37	theme	sugarcane	250:258	arg1	bagasse					260:266	sugarcane bagasse	250:266	sugarcane bagasse (SB)	250:271	The aim of this study was the application of an innovative dry chemo-mechanical pretreatment using different mechanical stresses to produce bioethanol from sugarcane bagasse (SB).
25656863	1	37	theme	sugarcane	250:258	arg1	SB					269:270	SB	269:270	SB	269:270	The aim of this study was the application of an innovative dry chemo-mechanical pretreatment using different mechanical stresses to produce bioethanol from sugarcane bagasse (SB).
25656863	4	38	dep	particles	597:605	arg1	size					607:610	size	607:610	size	607:610	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	4	38	dep	particles	597:605	arg1	crystallinity					626:638	cellulose crystallinity	616:638	cellulose crystallinity	616:638	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	4	38	dep	particles	597:605	arg1	particles					597:605	particles size and cellulose crystallinity	597:638	particles size and cellulose crystallinity	597:638	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	2	39	theme	energy	394:399	arg1	efficiency					401:410	energy efficiency	394:410	energy efficiency	394:410	The effect of different milling methods on physicochemical composition, enzymatic hydrolysis, bioethanol production and energy efficiency was also evaluated.
25656863	4	40	theme	cellulose	616:624	arg1	crystallinity					626:638	cellulose crystallinity	616:638	cellulose crystallinity	616:638	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	4	40	theme	cellulose	616:624	arg1	particles					597:605	particles size and cellulose crystallinity	597:638	particles size and cellulose crystallinity	597:638	Results indicate that vibratory milling (VBM) was more effective in the reduction of particles size and cellulose crystallinity compared to centrifugal (CM) and ball (BM) milling.
25656863	0	41	theme	sugarcane	75:83	arg1	bagasse					85:91	sugarcane bagasse	75:91	sugarcane bagasse	75:91	One-Pot dry chemo-mechanical deconstruction for bioethanol production from sugarcane bagasse.
25656863	7	42	from	SB	1085:1086	arg1	production					1055:1064	the production	1051:1064	the production of bioethanol from SB	1051:1086	Therefore, the combination of dry NaOH and CM appears the most suitable and interesting pretreatment for the production of bioethanol from SB.
25656863	1	43	theme	innovative	142:151	arg1	pretreatment					174:185	an innovative dry chemo-mechanical pretreatment	139:185	an innovative dry chemo-mechanical pretreatment using different mechanical stresses	139:221	The aim of this study was the application of an innovative dry chemo-mechanical pretreatment using different mechanical stresses to produce bioethanol from sugarcane bagasse (SB).
24274538	4	0	dep	Tl	451:452	arg1	PB					467:468	PB	467:468	probably PB	458:468	This means that the metal ion can be bound through two different sorption sites: one having a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material).
24274538	0	1	theme	alginate	56:63	arg1	capsules					65:72	alginate capsules	56:72	alginate capsules	56:72	Thallium(I) sorption using Prussian blue immobilized in alginate capsules.
24274538	6	2	theme	CaCl₂	743:747	arg1	addition					718:725	addition	718:725	addition of NaCl, KCl or CaCl₂	718:747	The ionic strength (increased by addition of NaCl, KCl or CaCl₂) slightly decreased sorption capacity.
24274538	3	3	theme	Langmuir	309:316	arg1	isotherm					327:334	the bi-site Langmuir sorption isotherm	297:334	the bi-site Langmuir sorption isotherm	297:334	The sorption isotherm can be described by the bi-site Langmuir sorption isotherm.
24274538	2	4	theme	optimum	226:232	arg1	pH					234:235	optimum pH	226:235	optimum pH	226:235	The composite sorbent was used for the recovery of Tl(I) ions from slightly acidic solutions: optimum pH being close to 4.
24274538	7	5	theme	capsules	824:831	arg1	analysis					800:807	The SEM-EDX analysis	788:807	The SEM-EDX analysis of PB-alginate capsules (before and after Tl(I) sorption)	788:865	The SEM-EDX analysis of PB-alginate capsules (before and after Tl(I) sorption) shows that the PB is homogeneously distributed in the capsules and that all reactive groups remain available for metal binding.
24274538	5	6	theme	pseudo-second	586:598	arg1	equation					611:618	the pseudo-second order rate equation	582:618	the pseudo-second order rate equation	582:618	The kinetics are described by either the pseudo-second order rate equation or the Crank's equation (resistance to intraparticle diffusion).
24274538	6	7	theme	KCl	736:738	arg1	addition					718:725	addition	718:725	addition of NaCl, KCl or CaCl₂	718:747	The ionic strength (increased by addition of NaCl, KCl or CaCl₂) slightly decreased sorption capacity.
24274538	5	8	theme	order	600:604	arg1	equation					611:618	the pseudo-second order rate equation	582:618	the pseudo-second order rate equation	582:618	The kinetics are described by either the pseudo-second order rate equation or the Crank's equation (resistance to intraparticle diffusion).
24274538	7	9	theme	SEM-EDX	792:798	arg1	analysis					800:807	The SEM-EDX analysis	788:807	The SEM-EDX analysis of PB-alginate capsules (before and after Tl(I) sorption)	788:865	The SEM-EDX analysis of PB-alginate capsules (before and after Tl(I) sorption) shows that the PB is homogeneously distributed in the capsules and that all reactive groups remain available for metal binding.
24274538	5	10	theme	rate	606:609	arg1	equation					611:618	the pseudo-second order rate equation	582:618	the pseudo-second order rate equation	582:618	The kinetics are described by either the pseudo-second order rate equation or the Crank's equation (resistance to intraparticle diffusion).
24274538	4	11	theme	sorption	402:409	arg1	sites					411:415	two different sorption sites	388:415	two different sorption sites	388:415	This means that the metal ion can be bound through two different sorption sites: one having a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material).
24274538	2	12	theme	acidic	208:213	arg1	solutions					215:223	slightly acidic solutions	199:223	slightly acidic solutions	199:223	The composite sorbent was used for the recovery of Tl(I) ions from slightly acidic solutions: optimum pH being close to 4.
24274538	3	13	theme	bi-site	301:307	arg1	isotherm					327:334	the bi-site Langmuir sorption isotherm	297:334	the bi-site Langmuir sorption isotherm	297:334	The sorption isotherm can be described by the bi-site Langmuir sorption isotherm.
24274538	4	14	theme	different	392:400	arg1	sites					411:415	two different sorption sites	388:415	two different sorption sites	388:415	This means that the metal ion can be bound through two different sorption sites: one having a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material).
24274538	7	15	theme	metal	980:984	arg1	binding					986:992	metal binding	980:992	metal binding	980:992	The SEM-EDX analysis of PB-alginate capsules (before and after Tl(I) sorption) shows that the PB is homogeneously distributed in the capsules and that all reactive groups remain available for metal binding.
24274538	0	16	theme	Thallium	0:7	arg1	sorption					12:19	Thallium(I) sorption	0:19	Thallium(I) sorption	0:19	Thallium(I) sorption using Prussian blue immobilized in alginate capsules.
24274538	5	17	dep	equation	635:642	arg1	resistance					645:654	resistance	645:654	resistance to intraparticle diffusion	645:681	The kinetics are described by either the pseudo-second order rate equation or the Crank's equation (resistance to intraparticle diffusion).
24274538	4	18	theme	encapsulating	520:532	arg1	material					534:541	the encapsulating material	516:541	probably the encapsulating material	507:541	This means that the metal ion can be bound through two different sorption sites: one having a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material).
24274538	5	19	theme	intraparticle	659:671	arg1	diffusion					673:681	intraparticle diffusion	659:681	intraparticle diffusion	659:681	The kinetics are described by either the pseudo-second order rate equation or the Crank's equation (resistance to intraparticle diffusion).
24274538	4	20	theme	lower	491:495	arg1	affinity					497:504	a lower affinity	489:504	a lower affinity (probably the encapsulating material)	489:542	This means that the metal ion can be bound through two different sorption sites: one having a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material).
24274538	6	21	dep	strength	695:702	arg1	increased					705:713	increased	705:713	increased by addition of NaCl, KCl or CaCl₂	705:747	The ionic strength (increased by addition of NaCl, KCl or CaCl₂) slightly decreased sorption capacity.
24274538	4	22	theme	strong	431:436	arg1	affinity					438:445	a strong affinity	429:445	a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material)	429:542	This means that the metal ion can be bound through two different sorption sites: one having a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material).
24274538	2	23	theme	composite	136:144	arg1	sorbent					146:152	The composite sorbent	132:152	The composite sorbent	132:152	The composite sorbent was used for the recovery of Tl(I) ions from slightly acidic solutions: optimum pH being close to 4.
24274538	4	24	dep	affinity	497:504	arg1	material					534:541	the encapsulating material	516:541	probably the encapsulating material	507:541	This means that the metal ion can be bound through two different sorption sites: one having a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material).
24274538	3	25	theme	sorption	318:325	arg1	isotherm					327:334	the bi-site Langmuir sorption isotherm	297:334	the bi-site Langmuir sorption isotherm	297:334	The sorption isotherm can be described by the bi-site Langmuir sorption isotherm.
24274538	3	26	theme	sorption	259:266	arg1	isotherm					268:275	The sorption isotherm	255:275	The sorption isotherm	255:275	The sorption isotherm can be described by the bi-site Langmuir sorption isotherm.
24274538	2	27	theme	Tl	183:184	arg1	ions					189:192	Tl(I) ions	183:192	Tl(I) ions	183:192	The composite sorbent was used for the recovery of Tl(I) ions from slightly acidic solutions: optimum pH being close to 4.
24274538	6	28	theme	NaCl	730:733	arg1	addition					718:725	addition	718:725	addition of NaCl, KCl or CaCl₂	718:747	The ionic strength (increased by addition of NaCl, KCl or CaCl₂) slightly decreased sorption capacity.
24274538	7	29	theme	reactive	943:950	arg1	groups					952:957	all reactive groups	939:957	all reactive groups	939:957	The SEM-EDX analysis of PB-alginate capsules (before and after Tl(I) sorption) shows that the PB is homogeneously distributed in the capsules and that all reactive groups remain available for metal binding.
24274538	6	30	theme	sorption	769:776	arg1	capacity					778:785	sorption capacity	769:785	sorption capacity	769:785	The ionic strength (increased by addition of NaCl, KCl or CaCl₂) slightly decreased sorption capacity.
24274538	2	31	from	solutions	215:223	arg1	recovery					171:178	the recovery	167:178	the recovery of Tl(I) ions from slightly acidic solutions	167:223	The composite sorbent was used for the recovery of Tl(I) ions from slightly acidic solutions: optimum pH being close to 4.
24274538	6	32	theme	ionic	689:693	arg1	strength					695:702	The ionic strength	685:702	The ionic strength (increased by addition of NaCl, KCl or CaCl₂)	685:748	The ionic strength (increased by addition of NaCl, KCl or CaCl₂) slightly decreased sorption capacity.
24274538	4	33	theme	metal	357:361	arg1	ion					363:365	the metal ion	353:365	the metal ion	353:365	This means that the metal ion can be bound through two different sorption sites: one having a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material).
24274538	4	34	contain	having	482:487	arg1	other					476:480	other	476:480	other	476:480	This means that the metal ion can be bound through two different sorption sites: one having a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material).
24274538	4	34	contain	having	482:487	arg1	Tl					451:452	Tl	451:452	Tl(I) (probably PB)	451:469	This means that the metal ion can be bound through two different sorption sites: one having a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material).
24274538	4	34	contain	having	482:487	arg2	affinity					497:504	a lower affinity	489:504	a lower affinity (probably the encapsulating material)	489:542	This means that the metal ion can be bound through two different sorption sites: one having a strong affinity for Tl(I) (probably PB), the other having a lower affinity (probably the encapsulating material).
24274538	2	35	used	used	158:161	arg2	sorbent					146:152	The composite sorbent	132:152	The composite sorbent	132:152	The composite sorbent was used for the recovery of Tl(I) ions from slightly acidic solutions: optimum pH being close to 4.
24274538	2	36	theme	ions	189:192	arg1	recovery					171:178	the recovery	167:178	the recovery of Tl(I) ions from slightly acidic solutions	167:223	The composite sorbent was used for the recovery of Tl(I) ions from slightly acidic solutions: optimum pH being close to 4.
24274538	0	37	theme	blue	36:39	arg1	Prussian					27:34	Prussian blue	27:39	Prussian blue immobilized in alginate capsules	27:72	Thallium(I) sorption using Prussian blue immobilized in alginate capsules.
24274538	7	38	theme	PB-alginate	812:822	arg1	sorption					857:864	sorption	857:864	sorption	857:864	The SEM-EDX analysis of PB-alginate capsules (before and after Tl(I) sorption) shows that the PB is homogeneously distributed in the capsules and that all reactive groups remain available for metal binding.
24274538	7	38	theme	PB-alginate	812:822	arg1	capsules					824:831	PB-alginate capsules	812:831	PB-alginate capsules (before and after Tl(I) sorption)	812:865	The SEM-EDX analysis of PB-alginate capsules (before and after Tl(I) sorption) shows that the PB is homogeneously distributed in the capsules and that all reactive groups remain available for metal binding.
24274538	1	39	theme	alginate	113:120	arg1	capsules					122:129	alginate capsules	113:129	alginate capsules	113:129	Prussian blue (PB) was immobilized in alginate capsules.
27810730	9	0	theme	nucleus	1303:1309	arg1	pulposus					1311:1318	the native nucleus pulposus	1292:1318	the native nucleus pulposus	1292:1318	The incorporation of polymeric reinforcement within the gels increased material stiffness to that comparable to the native nucleus pulposus, however permeability was significantly greater than native values.
27810730	1	1	contain	has	129:131	arg2	effect					159:164	an immensely debilitating effect	133:164	an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world	133:257	Severe degeneration of the intervertebral disc has an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world.
27810730	1	1	contain	has	129:131	arg1	degeneration					89:100	Severe degeneration	82:100	Severe degeneration of the intervertebral disc	82:127	Severe degeneration of the intervertebral disc has an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world.
27810730	7	2	theme	pulposus	1042:1049	arg1	engineering					1058:1068	nucleus pulposus tissue engineering	1034:1068	nucleus pulposus tissue engineering	1034:1068	This study investigates the potential of a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering.
27810730	10	3	theme	cell	1402:1405	arg1	evaluation					1407:1416	A preliminary cell evaluation	1388:1416	A preliminary cell evaluation culturing NIH 3T3s over 21 days suggested the incorporation of polymeric networks	1388:1498	A preliminary cell evaluation culturing NIH 3T3s over 21 days suggested the incorporation of polymeric networks also enhanced cellular proliferation compared to gels alone.
27810730	9	4	theme	native	1373:1378	arg1	values					1380:1385	native values	1373:1385	native values	1373:1385	The incorporation of polymeric reinforcement within the gels increased material stiffness to that comparable to the native nucleus pulposus, however permeability was significantly greater than native values.
27810730	7	5	theme	tissue	1051:1056	arg1	engineering					1058:1068	nucleus pulposus tissue engineering	1034:1068	nucleus pulposus tissue engineering	1034:1068	This study investigates the potential of a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering.
27810730	2	6	theme	integral	278:285	arg1	role					287:290	an integral role	275:290	an integral role	275:290	The disc plays an integral role in biomechanical movement and support within the spine.
27810730	7	7	theme	carrageenan	980:990	arg1	scaffold					1021:1028	a carrageenan gel-infused polycaprolactone scaffold	978:1028	a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering	978:1068	This study investigates the potential of a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering.
27810730	8	8	dep	viscoelastic	1118:1129	arg1	frameworks					1147:1156	frameworks	1147:1156	frameworks	1147:1156	Mechanical properties were characterised using viscoelastic and poroelastic frameworks via microindentation.
27810730	4	9	theme	architectural	595:607	arg1	resemblance					609:619	its architectural resemblance	591:619	its architectural resemblance	591:619	Hydrogels have been widely investigated as a candidate for regeneration of the gelatinous nucleus pulposus due to its architectural resemblance and fluid retention characteristics.
27810730	5	10	theme	extrusion	762:770	arg1	complications					772:784	extrusion complications	762:784	extrusion complications	762:784	However, hydrogels are often limited due to small compressive stiffness and tear resistance, leading to extrusion complications.
27810730	1	11	from	effect	159:164	arg1	quality					169:175	quality	169:175	quality of life	169:183	Severe degeneration of the intervertebral disc has an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world.
27810730	9	12	theme	polymeric	1201:1209	arg1	reinforcement					1211:1223	polymeric reinforcement	1201:1223	polymeric reinforcement	1201:1223	The incorporation of polymeric reinforcement within the gels increased material stiffness to that comparable to the native nucleus pulposus, however permeability was significantly greater than native values.
27810730	6	13	theme	polymeric	831:839	arg1	scaffolds					841:849	polymeric scaffolds	831:849	polymeric scaffolds	831:849	Reinforcement of the hydrogel network using polymeric scaffolds may address these issues of inadequate mechanical properties and implant instability.
27810730	1	14	theme	Severe	82:87	arg1	degeneration					89:100	Severe degeneration	82:100	Severe degeneration of the intervertebral disc	82:127	Severe degeneration of the intervertebral disc has an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world.
27810730	9	15	theme	reinforcement	1211:1223	arg1	incorporation					1184:1196	The incorporation	1180:1196	The incorporation of polymeric reinforcement within the gels	1180:1239	The incorporation of polymeric reinforcement within the gels increased material stiffness to that comparable to the native nucleus pulposus, however permeability was significantly greater than native values.
27810730	1	16	theme	life	180:183	arg1	quality					169:175	quality	169:175	quality of life	169:183	Severe degeneration of the intervertebral disc has an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world.
27810730	0	17	theme	interpenetrating	3:18	arg1	network					20:26	An interpenetrating network	0:26	An interpenetrating network	0:26	An interpenetrating network composite for a regenerative spinal disc application.
27810730	7	18	theme	scaffold	1021:1028	arg1	potential					965:973	the potential	961:973	the potential of a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering	961:1068	This study investigates the potential of a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering.
27810730	10	19	theme	NIH	1428:1430	arg1	3T3s					1432:1435	NIH 3T3s	1428:1435	NIH 3T3s	1428:1435	A preliminary cell evaluation culturing NIH 3T3s over 21 days suggested the incorporation of polymeric networks also enhanced cellular proliferation compared to gels alone.
27810730	4	20	theme	nucleus	567:573	arg1	pulposus					575:582	the gelatinous nucleus pulposus	552:582	the gelatinous nucleus pulposus due to its architectural resemblance and fluid retention characteristics	552:655	Hydrogels have been widely investigated as a candidate for regeneration of the gelatinous nucleus pulposus due to its architectural resemblance and fluid retention characteristics.
27810730	6	21	theme	network	817:823	arg1	Reinforcement					787:799	Reinforcement	787:799	Reinforcement of the hydrogel network using polymeric scaffolds	787:849	Reinforcement of the hydrogel network using polymeric scaffolds may address these issues of inadequate mechanical properties and implant instability.
27810730	2	22	theme	biomechanical	295:307	arg1	movement					309:316	biomechanical movement	295:316	biomechanical movement	295:316	The disc plays an integral role in biomechanical movement and support within the spine.
27810730	4	23	theme	gelatinous	556:565	arg1	pulposus					575:582	the gelatinous nucleus pulposus	552:582	the gelatinous nucleus pulposus due to its architectural resemblance and fluid retention characteristics	552:655	Hydrogels have been widely investigated as a candidate for regeneration of the gelatinous nucleus pulposus due to its architectural resemblance and fluid retention characteristics.
27810730	6	24	theme	hydrogel	808:815	arg1	network					817:823	the hydrogel network	804:823	the hydrogel network using polymeric scaffolds	804:849	Reinforcement of the hydrogel network using polymeric scaffolds may address these issues of inadequate mechanical properties and implant instability.
27810730	3	25	theme	tissue	469:474	arg1	functionality					441:453	functionality	441:453	functionality of the native tissue	441:474	The emergence of tissue engineering endeavours to restore the structural characteristics and functionality of the native tissue.
27810730	3	25	theme	tissue	469:474	arg1	characteristics					421:435	the structural characteristics	406:435	the structural characteristics	406:435	The emergence of tissue engineering endeavours to restore the structural characteristics and functionality of the native tissue.
27810730	3	26	theme	tissue	365:370	arg1	engineering					372:382	tissue engineering	365:382	tissue engineering	365:382	The emergence of tissue engineering endeavours to restore the structural characteristics and functionality of the native tissue.
27810730	4	27	theme	due	584:586	arg1	pulposus					575:582	the gelatinous nucleus pulposus	552:582	the gelatinous nucleus pulposus due to its architectural resemblance and fluid retention characteristics	552:655	Hydrogels have been widely investigated as a candidate for regeneration of the gelatinous nucleus pulposus due to its architectural resemblance and fluid retention characteristics.
27810730	5	28	theme	tear	734:737	arg1	resistance					739:748	tear resistance	734:748	tear resistance	734:748	However, hydrogels are often limited due to small compressive stiffness and tear resistance, leading to extrusion complications.
27810730	4	29	dep	resemblance	609:619	arg1	characteristics					641:655	characteristics	641:655	characteristics	641:655	Hydrogels have been widely investigated as a candidate for regeneration of the gelatinous nucleus pulposus due to its architectural resemblance and fluid retention characteristics.
27810730	1	30	theme	intervertebral	109:122	arg1	disc					124:127	the intervertebral disc	105:127	the intervertebral disc	105:127	Severe degeneration of the intervertebral disc has an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world.
27810730	7	31	theme	nucleus	1034:1040	arg1	engineering					1058:1068	nucleus pulposus tissue engineering	1034:1068	nucleus pulposus tissue engineering	1034:1068	This study investigates the potential of a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering.
27810730	4	32	theme	pulposus	575:582	arg1	regeneration					536:547	regeneration	536:547	regeneration of the gelatinous nucleus pulposus due to its architectural resemblance and fluid retention characteristics	536:655	Hydrogels have been widely investigated as a candidate for regeneration of the gelatinous nucleus pulposus due to its architectural resemblance and fluid retention characteristics.
27810730	9	33	theme	material	1251:1258	arg1	stiffness					1260:1268	material stiffness	1251:1268	material stiffness	1251:1268	The incorporation of polymeric reinforcement within the gels increased material stiffness to that comparable to the native nucleus pulposus, however permeability was significantly greater than native values.
27810730	1	34	theme	serious	203:209	arg1	health					211:216	a serious health	201:216	a serious health	201:216	Severe degeneration of the intervertebral disc has an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world.
27810730	3	35	theme	structural	410:419	arg1	characteristics					421:435	the structural characteristics	406:435	the structural characteristics	406:435	The emergence of tissue engineering endeavours to restore the structural characteristics and functionality of the native tissue.
27810730	3	36	theme	native	462:467	arg1	tissue					469:474	the native tissue	458:474	the native tissue	458:474	The emergence of tissue engineering endeavours to restore the structural characteristics and functionality of the native tissue.
27810730	0	37	theme	spinal	57:62	arg1	application					69:79	a regenerative spinal disc application	42:79	a regenerative spinal disc application	42:79	An interpenetrating network composite for a regenerative spinal disc application.
27810730	6	38	theme	properties	901:910	arg1	issues					869:874	these issues	863:874	these issues of inadequate mechanical properties	863:910	Reinforcement of the hydrogel network using polymeric scaffolds may address these issues of inadequate mechanical properties and implant instability.
27810730	10	39	theme	networks	1491:1498	arg1	incorporation					1464:1476	the incorporation	1460:1476	the incorporation of polymeric networks	1460:1498	A preliminary cell evaluation culturing NIH 3T3s over 21 days suggested the incorporation of polymeric networks also enhanced cellular proliferation compared to gels alone.
27810730	1	40	theme	disc	124:127	arg1	degeneration					89:100	Severe degeneration	82:100	Severe degeneration of the intervertebral disc	82:127	Severe degeneration of the intervertebral disc has an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world.
27810730	0	41	theme	regenerative	44:55	arg1	application					69:79	a regenerative spinal disc application	42:79	a regenerative spinal disc application	42:79	An interpenetrating network composite for a regenerative spinal disc application.
27810730	5	42	theme	small	702:706	arg1	stiffness					720:728	small compressive stiffness	702:728	small compressive stiffness	702:728	However, hydrogels are often limited due to small compressive stiffness and tear resistance, leading to extrusion complications.
27810730	6	43	theme	mechanical	890:899	arg1	properties					901:910	inadequate mechanical properties	879:910	inadequate mechanical properties	879:910	Reinforcement of the hydrogel network using polymeric scaffolds may address these issues of inadequate mechanical properties and implant instability.
27810730	7	44	theme	polycaprolactone	1004:1019	arg1	scaffold					1021:1028	a carrageenan gel-infused polycaprolactone scaffold	978:1028	a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering	978:1068	This study investigates the potential of a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering.
27810730	10	45	theme	polymeric	1481:1489	arg1	networks					1491:1498	polymeric networks	1481:1498	polymeric networks	1481:1498	A preliminary cell evaluation culturing NIH 3T3s over 21 days suggested the incorporation of polymeric networks also enhanced cellular proliferation compared to gels alone.
27810730	7	46	theme	gel-infused	992:1002	arg1	scaffold					1021:1028	a carrageenan gel-infused polycaprolactone scaffold	978:1028	a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering	978:1068	This study investigates the potential of a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering.
27810730	5	47	theme	compressive	708:718	arg1	stiffness					720:728	small compressive stiffness	702:728	small compressive stiffness	702:728	However, hydrogels are often limited due to small compressive stiffness and tear resistance, leading to extrusion complications.
27810730	6	48	theme	inadequate	879:888	arg1	properties					901:910	inadequate mechanical properties	879:910	inadequate mechanical properties	879:910	Reinforcement of the hydrogel network using polymeric scaffolds may address these issues of inadequate mechanical properties and implant instability.
27810730	8	49	theme	Mechanical	1071:1080	arg1	properties					1082:1091	Mechanical properties	1071:1091	Mechanical properties	1071:1091	Mechanical properties were characterised using viscoelastic and poroelastic frameworks via microindentation.
27810730	10	50	theme	cellular	1514:1521	arg1	proliferation					1523:1535	cellular proliferation	1514:1535	cellular proliferation	1514:1535	A preliminary cell evaluation culturing NIH 3T3s over 21 days suggested the incorporation of polymeric networks also enhanced cellular proliferation compared to gels alone.
27810730	4	51	theme	fluid	625:629	arg1	retention					631:639	fluid retention	625:639	fluid retention	625:639	Hydrogels have been widely investigated as a candidate for regeneration of the gelatinous nucleus pulposus due to its architectural resemblance and fluid retention characteristics.
27810730	9	52	dep	pulposus	1311:1318	arg1	greater					1360:1366	greater	1360:1366	greater	1360:1366	The incorporation of polymeric reinforcement within the gels increased material stiffness to that comparable to the native nucleus pulposus, however permeability was significantly greater than native values.
27810730	1	53	theme	economic	222:229	arg1	burden					231:236	economic burden	222:236	economic burden	222:236	Severe degeneration of the intervertebral disc has an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world.
27810730	10	54	theme	preliminary	1390:1400	arg1	evaluation					1407:1416	A preliminary cell evaluation	1388:1416	A preliminary cell evaluation culturing NIH 3T3s over 21 days suggested the incorporation of polymeric networks	1388:1498	A preliminary cell evaluation culturing NIH 3T3s over 21 days suggested the incorporation of polymeric networks also enhanced cellular proliferation compared to gels alone.
27810730	1	55	theme	debilitating	146:157	arg1	effect					159:164	an immensely debilitating effect	133:164	an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world	133:257	Severe degeneration of the intervertebral disc has an immensely debilitating effect on quality of life that has become a serious health and economic burden throughout the world.
27810730	0	56	theme	disc	64:67	arg1	application					69:79	a regenerative spinal disc application	42:79	a regenerative spinal disc application	42:79	An interpenetrating network composite for a regenerative spinal disc application.
27810730	9	57	theme	native	1296:1301	arg1	pulposus					1311:1318	the native nucleus pulposus	1292:1318	the native nucleus pulposus	1292:1318	The incorporation of polymeric reinforcement within the gels increased material stiffness to that comparable to the native nucleus pulposus, however permeability was significantly greater than native values.
27810730	3	58	theme	engineering	372:382	arg1	emergence					352:360	The emergence	348:360	The emergence of tissue engineering	348:382	The emergence of tissue engineering endeavours to restore the structural characteristics and functionality of the native tissue.
28543403	4	0	theme	quantitative	720:731	arg1	specifications					740:753	quantitative design specifications	720:753	quantitative design specifications for fabricating scaffolds for hepatic tissue engineering using human livers as a template	720:843	The intention of this work was to obtain quantitative design specifications for fabricating scaffolds for hepatic tissue engineering using human livers as a template.
28543403	1	1	theme	extracellular	172:184	arg1	ECM					194:196	ECM	194:196	ECM	194:196	Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved.
28543403	1	1	theme	extracellular	172:184	arg1	matrix					186:191	the perfect extracellular matrix	160:191	the perfect extracellular matrix (ECM)	160:197	Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved.
28543403	6	2	theme	decellularization	1055:1071	arg1	outcome					1073:1079	The decellularization outcome	1051:1079	The decellularization outcome	1051:1079	The decellularization outcome was evaluated through histological and quantitative image analyses to evaluate cell removal, protein, and glycosaminoglycan content per unit area.
28543403	0	3	theme	Extracellular	72:84	arg1	Matrix					86:91	a Generic Extracellular Matrix Mimic Hepatic Scaffold	62:114	a Generic Extracellular Matrix Mimic Hepatic Scaffold	62:114	Decellularized Human Liver Is Too Heterogeneous for Designing a Generic Extracellular Matrix Mimic Hepatic Scaffold.
28543403	1	4	theme	hepatic	284:290	arg1	microenvironment					292:307	the hepatic microenvironment	280:307	the hepatic microenvironment	280:307	Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved.
28543403	0	5	theme	Hepatic	99:105	arg1	Scaffold					107:114	Hepatic Scaffold	99:114	a Generic Extracellular Matrix Mimic Hepatic Scaffold	62:114	Decellularized Human Liver Is Too Heterogeneous for Designing a Generic Extracellular Matrix Mimic Hepatic Scaffold.
28543403	8	6	theme	matrix	1493:1498	arg1	levels					1463:1468	different levels	1453:1468	different levels of remaining cells and matrix	1453:1498	Only one liver out of five was completely decellularized, while the other four showed different levels of remaining cells and matrix.
28543403	3	7	theme	structural	556:565	arg1	properties					583:592	the structural and biochemical properties	552:592	the structural and biochemical properties of human hepatic ECM	552:613	It is hence of interest to determine the structural and biochemical properties of human hepatic ECM to derive design criteria for engineering biomimetic scaffolds.
28543403	5	8	from	microstructure	1017:1030	arg1	livers					1043:1048	porcine livers	1035:1048	porcine livers	1035:1048	To this end, hepatic samples from five patients scheduled for hepatic resection were decellularized using a protocol shown to reproducibly conserve matrix composition and microstructure in porcine livers.
28543403	1	9	theme	microenvironment	292:307	arg1	features					268:275	biological features	257:275	biological features of the hepatic microenvironment	257:307	Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved.
28543403	1	9	theme	microenvironment	292:307	arg1	architecture					240:251	three-dimensional architecture	222:251	three-dimensional architecture	222:251	Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved.
28543403	2	10	theme	donor	347:351	arg1	livers					359:364	donor human livers	347:364	donor human livers	347:364	However, donor human livers are in chronically short supply, both for transplantation or as decellularized scaffolds, and will become even scarcer as life expectancy increases.
28543403	2	11	theme	short	385:389	arg1	supply					391:396	chronically short supply	373:396	chronically short supply	373:396	However, donor human livers are in chronically short supply, both for transplantation or as decellularized scaffolds, and will become even scarcer as life expectancy increases.
28543403	9	12	theme	unit	1553:1556	arg1	area					1558:1561	unit area	1553:1561	unit area after decellularization	1553:1585	Moreover, protein and glycosaminoglycan content per unit area after decellularization were also found to be patient- (or donor-) dependent.
28543403	9	13	theme	protein	1511:1517	arg1	content					1541:1547	protein and glycosaminoglycan content	1511:1547	content	1541:1547	Moreover, protein and glycosaminoglycan content per unit area after decellularization were also found to be patient- (or donor-) dependent.
28543403	10	14	theme	donor-to-donor	1646:1659	arg1	variability					1661:1671	This donor-to-donor variability	1641:1671	This donor-to-donor variability of human livers	1641:1687	This donor-to-donor variability of human livers thus precludes their use as templates for engineering a generic "one-size fits all" ECM-mimic hepatic scaffold.
28543403	10	15	theme	generic	1745:1751	arg1	one-size					1754:1761	a generic "one-size	1743:1761	a generic "one-size fits all" ECM-mimic hepatic scaffold	1743:1798	This donor-to-donor variability of human livers thus precludes their use as templates for engineering a generic "one-size fits all" ECM-mimic hepatic scaffold.
28543403	7	16	theme	human	1276:1280	arg1	samples					1288:1294	human liver samples	1276:1294	human liver samples obtained from five different patients	1276:1332	Applying the same decellularization protocol to human liver samples obtained from five different patients yielded five different outcomes.
28543403	10	17	theme	ECM-mimic	1773:1781	arg1	scaffold					1791:1798	all" ECM-mimic hepatic scaffold	1768:1798	all" ECM-mimic hepatic scaffold	1768:1798	This donor-to-donor variability of human livers thus precludes their use as templates for engineering a generic "one-size fits all" ECM-mimic hepatic scaffold.
28543403	6	18	theme	glycosaminoglycan	1187:1203	arg1	content					1205:1211	glycosaminoglycan content	1187:1211	glycosaminoglycan content per unit area	1187:1225	The decellularization outcome was evaluated through histological and quantitative image analyses to evaluate cell removal, protein, and glycosaminoglycan content per unit area.
28543403	4	19	theme	human	818:822	arg1	template					836:843	a template	834:843	a template	834:843	The intention of this work was to obtain quantitative design specifications for fabricating scaffolds for hepatic tissue engineering using human livers as a template.
28543403	4	19	theme	human	818:822	arg1	livers					824:829	human livers	818:829	human livers	818:829	The intention of this work was to obtain quantitative design specifications for fabricating scaffolds for hepatic tissue engineering using human livers as a template.
28543403	9	20	theme	glycosaminoglycan	1523:1539	arg1	content					1541:1547	protein and glycosaminoglycan content	1511:1547	content	1541:1547	Moreover, protein and glycosaminoglycan content per unit area after decellularization were also found to be patient- (or donor-) dependent.
28543403	0	21	theme	Human	15:19	arg1	Liver					21:25	Decellularized Human Liver	0:25	Decellularized Human Liver	0:25	Decellularized Human Liver Is Too Heterogeneous for Designing a Generic Extracellular Matrix Mimic Hepatic Scaffold.
28543403	0	22	dep	Matrix	86:91	arg1	Scaffold					107:114	Hepatic Scaffold	99:114	a Generic Extracellular Matrix Mimic Hepatic Scaffold	62:114	Decellularized Human Liver Is Too Heterogeneous for Designing a Generic Extracellular Matrix Mimic Hepatic Scaffold.
28543403	0	22	dep	Matrix	86:91	arg1	Mimic					93:97	Mimic	93:97	Mimic	93:97	Decellularized Human Liver Is Too Heterogeneous for Designing a Generic Extracellular Matrix Mimic Hepatic Scaffold.
28543403	7	23	theme	decellularization	1246:1262	arg1	protocol					1264:1271	the same decellularization protocol	1237:1271	the same decellularization protocol	1237:1271	Applying the same decellularization protocol to human liver samples obtained from five different patients yielded five different outcomes.
28543403	4	24	theme	work	701:704	arg1	intention					683:691	The intention	679:691	The intention of this work	679:704	The intention of this work was to obtain quantitative design specifications for fabricating scaffolds for hepatic tissue engineering using human livers as a template.
28543403	0	25	theme	Decellularized	0:13	arg1	Liver					21:25	Decellularized Human Liver	0:25	Decellularized Human Liver	0:25	Decellularized Human Liver Is Too Heterogeneous for Designing a Generic Extracellular Matrix Mimic Hepatic Scaffold.
28543403	10	26	theme	"	1771:1771	arg1	scaffold					1791:1798	all" ECM-mimic hepatic scaffold	1768:1798	all" ECM-mimic hepatic scaffold	1768:1798	This donor-to-donor variability of human livers thus precludes their use as templates for engineering a generic "one-size fits all" ECM-mimic hepatic scaffold.
28543403	3	27	theme	human	597:601	arg1	ECM					611:613	human hepatic ECM	597:613	human hepatic ECM	597:613	It is hence of interest to determine the structural and biochemical properties of human hepatic ECM to derive design criteria for engineering biomimetic scaffolds.
28543403	5	28	theme	hepatic	908:914	arg1	resection					916:924	hepatic resection	908:924	hepatic resection	908:924	To this end, hepatic samples from five patients scheduled for hepatic resection were decellularized using a protocol shown to reproducibly conserve matrix composition and microstructure in porcine livers.
28543403	10	29	theme	livers	1682:1687	arg1	variability					1661:1671	This donor-to-donor variability	1641:1671	This donor-to-donor variability of human livers	1641:1687	This donor-to-donor variability of human livers thus precludes their use as templates for engineering a generic "one-size fits all" ECM-mimic hepatic scaffold.
28543403	3	30	theme	biochemical	571:581	arg1	properties					583:592	the structural and biochemical properties	552:592	the structural and biochemical properties of human hepatic ECM	552:613	It is hence of interest to determine the structural and biochemical properties of human hepatic ECM to derive design criteria for engineering biomimetic scaffolds.
28543403	10	31	dep	one-size	1754:1761	arg1	fits					1763:1766	fits	1763:1766	fits all" ECM-mimic hepatic scaffold	1763:1798	This donor-to-donor variability of human livers thus precludes their use as templates for engineering a generic "one-size fits all" ECM-mimic hepatic scaffold.
28543403	5	32	theme	hepatic	859:865	arg1	samples					867:873	hepatic samples	859:873	hepatic samples from five patients scheduled for hepatic resection	859:924	To this end, hepatic samples from five patients scheduled for hepatic resection were decellularized using a protocol shown to reproducibly conserve matrix composition and microstructure in porcine livers.
28543403	7	33	theme	same	1241:1244	arg1	protocol					1264:1271	the same decellularization protocol	1237:1271	the same decellularization protocol	1237:1271	Applying the same decellularization protocol to human liver samples obtained from five different patients yielded five different outcomes.
28543403	4	34	theme	hepatic	785:791	arg1	engineering					800:810	hepatic tissue engineering	785:810	hepatic tissue engineering using human livers as a template	785:843	The intention of this work was to obtain quantitative design specifications for fabricating scaffolds for hepatic tissue engineering using human livers as a template.
28543403	6	35	theme	quantitative	1120:1131	arg1	analyses					1139:1146	histological and quantitative image analyses	1103:1146	histological and quantitative image analyses	1103:1146	The decellularization outcome was evaluated through histological and quantitative image analyses to evaluate cell removal, protein, and glycosaminoglycan content per unit area.
28543403	7	36	theme	liver	1282:1286	arg1	samples					1288:1294	human liver samples	1276:1294	human liver samples obtained from five different patients	1276:1332	Applying the same decellularization protocol to human liver samples obtained from five different patients yielded five different outcomes.
28543403	4	37	theme	design	733:738	arg1	specifications					740:753	quantitative design specifications	720:753	quantitative design specifications for fabricating scaffolds for hepatic tissue engineering using human livers as a template	720:843	The intention of this work was to obtain quantitative design specifications for fabricating scaffolds for hepatic tissue engineering using human livers as a template.
28543403	1	38	theme	three-dimensional	222:238	arg1	architecture					240:251	three-dimensional architecture	222:251	three-dimensional architecture	222:251	Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved.
28543403	8	39	theme	cells	1483:1487	arg1	levels					1463:1468	different levels	1453:1468	different levels of remaining cells and matrix	1453:1498	Only one liver out of five was completely decellularized, while the other four showed different levels of remaining cells and matrix.
28543403	2	40	theme	decellularized	430:443	arg1	scaffolds					445:453	decellularized scaffolds	430:453	decellularized scaffolds	430:453	However, donor human livers are in chronically short supply, both for transplantation or as decellularized scaffolds, and will become even scarcer as life expectancy increases.
28543403	2	41	theme	life	488:491	arg1	expectancy					493:502	life expectancy	488:502	life expectancy	488:502	However, donor human livers are in chronically short supply, both for transplantation or as decellularized scaffolds, and will become even scarcer as life expectancy increases.
28543403	3	42	theme	biomimetic	657:666	arg1	scaffolds					668:676	biomimetic scaffolds	657:676	biomimetic scaffolds	657:676	It is hence of interest to determine the structural and biochemical properties of human hepatic ECM to derive design criteria for engineering biomimetic scaffolds.
28543403	8	43	theme	remaining	1473:1481	arg1	cells					1483:1487	remaining cells	1473:1487	remaining cells	1473:1487	Only one liver out of five was completely decellularized, while the other four showed different levels of remaining cells and matrix.
28543403	6	44	theme	histological	1103:1114	arg1	analyses					1139:1146	histological and quantitative image analyses	1103:1146	histological and quantitative image analyses	1103:1146	The decellularization outcome was evaluated through histological and quantitative image analyses to evaluate cell removal, protein, and glycosaminoglycan content per unit area.
28543403	6	45	dep	cell	1160:1163	arg1	removal					1165:1171	removal	1165:1171	removal	1165:1171	The decellularization outcome was evaluated through histological and quantitative image analyses to evaluate cell removal, protein, and glycosaminoglycan content per unit area.
28543403	8	46	theme	different	1453:1461	arg1	levels					1463:1468	different levels	1453:1468	different levels of remaining cells and matrix	1453:1498	Only one liver out of five was completely decellularized, while the other four showed different levels of remaining cells and matrix.
28543403	3	47	theme	hepatic	603:609	arg1	ECM					611:613	human hepatic ECM	597:613	human hepatic ECM	597:613	It is hence of interest to determine the structural and biochemical properties of human hepatic ECM to derive design criteria for engineering biomimetic scaffolds.
28543403	1	48	theme	Decellularized	117:130	arg1	livers					138:143	Decellularized human livers	117:143	Decellularized human livers	117:143	Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved.
28543403	5	49	theme	matrix	994:999	arg1	composition					1001:1011	matrix composition	994:1011	matrix composition	994:1011	To this end, hepatic samples from five patients scheduled for hepatic resection were decellularized using a protocol shown to reproducibly conserve matrix composition and microstructure in porcine livers.
28543403	3	50	theme	ECM	611:613	arg1	properties					583:592	the structural and biochemical properties	552:592	the structural and biochemical properties of human hepatic ECM	552:613	It is hence of interest to determine the structural and biochemical properties of human hepatic ECM to derive design criteria for engineering biomimetic scaffolds.
28543403	1	51	theme	human	132:136	arg1	livers					138:143	Decellularized human livers	117:143	Decellularized human livers	117:143	Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved.
28543403	7	52	theme	different	1347:1355	arg1	outcomes					1357:1364	five different outcomes	1342:1364	five different outcomes	1342:1364	Applying the same decellularization protocol to human liver samples obtained from five different patients yielded five different outcomes.
28543403	4	53	theme	fabricating	759:769	arg1	scaffolds					771:779	fabricating scaffolds	759:779	fabricating scaffolds	759:779	The intention of this work was to obtain quantitative design specifications for fabricating scaffolds for hepatic tissue engineering using human livers as a template.
28543403	4	54	theme	tissue	793:798	arg1	engineering					800:810	hepatic tissue engineering	785:810	hepatic tissue engineering using human livers as a template	785:843	The intention of this work was to obtain quantitative design specifications for fabricating scaffolds for hepatic tissue engineering using human livers as a template.
28543403	5	55	theme	porcine	1035:1041	arg1	livers					1043:1048	porcine livers	1035:1048	porcine livers	1035:1048	To this end, hepatic samples from five patients scheduled for hepatic resection were decellularized using a protocol shown to reproducibly conserve matrix composition and microstructure in porcine livers.
28543403	2	56	theme	human	353:357	arg1	livers					359:364	donor human livers	347:364	donor human livers	347:364	However, donor human livers are in chronically short supply, both for transplantation or as decellularized scaffolds, and will become even scarcer as life expectancy increases.
28543403	1	57	theme	biological	257:266	arg1	features					268:275	biological features	257:275	biological features of the hepatic microenvironment	257:307	Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved.
28543403	0	58	theme	Generic	64:70	arg1	Matrix					86:91	a Generic Extracellular Matrix Mimic Hepatic Scaffold	62:114	a Generic Extracellular Matrix Mimic Hepatic Scaffold	62:114	Decellularized Human Liver Is Too Heterogeneous for Designing a Generic Extracellular Matrix Mimic Hepatic Scaffold.
28543403	10	59	theme	hepatic	1783:1789	arg1	scaffold					1791:1798	all" ECM-mimic hepatic scaffold	1768:1798	all" ECM-mimic hepatic scaffold	1768:1798	This donor-to-donor variability of human livers thus precludes their use as templates for engineering a generic "one-size fits all" ECM-mimic hepatic scaffold.
28543403	3	60	theme	design	625:630	arg1	criteria					632:639	design criteria	625:639	design criteria for engineering biomimetic scaffolds	625:676	It is hence of interest to determine the structural and biochemical properties of human hepatic ECM to derive design criteria for engineering biomimetic scaffolds.
28543403	6	61	theme	image	1133:1137	arg1	analyses					1139:1146	histological and quantitative image analyses	1103:1146	histological and quantitative image analyses	1103:1146	The decellularization outcome was evaluated through histological and quantitative image analyses to evaluate cell removal, protein, and glycosaminoglycan content per unit area.
28543403	5	62	from	composition	1001:1011	arg1	livers					1043:1048	porcine livers	1035:1048	porcine livers	1035:1048	To this end, hepatic samples from five patients scheduled for hepatic resection were decellularized using a protocol shown to reproducibly conserve matrix composition and microstructure in porcine livers.
28543403	7	63	theme	different	1315:1323	arg1	patients					1325:1332	five different patients	1310:1332	five different patients	1310:1332	Applying the same decellularization protocol to human liver samples obtained from five different patients yielded five different outcomes.
28543403	6	64	theme	unit	1217:1220	arg1	area					1222:1225	unit area	1217:1225	unit area	1217:1225	The decellularization outcome was evaluated through histological and quantitative image analyses to evaluate cell removal, protein, and glycosaminoglycan content per unit area.
28543403	10	65	theme	human	1676:1680	arg1	livers					1682:1687	human livers	1676:1687	human livers	1676:1687	This donor-to-donor variability of human livers thus precludes their use as templates for engineering a generic "one-size fits all" ECM-mimic hepatic scaffold.
28543403	5	66	from	patients	885:892	arg1	samples					867:873	hepatic samples	859:873	hepatic samples from five patients scheduled for hepatic resection	859:924	To this end, hepatic samples from five patients scheduled for hepatic resection were decellularized using a protocol shown to reproducibly conserve matrix composition and microstructure in porcine livers.
28543403	1	67	theme	the perfect	160:170	arg1	ECM					194:196	ECM	194:196	ECM	194:196	Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved.
28543403	1	67	theme	the perfect	160:170	arg1	matrix					186:191	the perfect extracellular matrix	160:191	the perfect extracellular matrix (ECM)	160:197	Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved.
26403803	7	0	from	pasta	1227:1231	arg1	significant					1291:1301	significant	1291:1301	significant	1291:1301	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	7	0	from	pasta	1227:1231	arg1	decrease					1249:1256	the decrease	1245:1256	the decrease of saturated fatty acids	1245:1281	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	6	1	theme	acids	1023:1027	arg1	losses					1007:1012	losses	1007:1012	losses of amino acids and tocopherols	1007:1043	Cooking decreased all of the metabolites: the high-fibre pasta (A-C) and Control showed losses of amino acids and tocopherols, while for sugars and organic acids, the decrease depended on the pasta sample.
26403803	3	2	theme	available	652:660	arg1	pasta					673:677	the commercially available high-fibre pasta	635:677	the commercially available high-fibre pasta (samples A-C)	635:691	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	3	2	theme	available	652:660	arg1	A-C					688:690	samples A-C	680:690	samples A-C	680:690	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	2	3	from	metabolites	293:303	arg1	samples					324:330	different pasta samples	308:330	different pasta samples	308:330	The aim of the present study was to determine and compare the metabolites in different pasta samples with fibre, and to investigate the modifications induced in these different kinds of pasta during cooking, using a gas chromatography-mass spectrometry-based metabolomics approach.
26403803	4	4	theme	enriched	786:793	arg1	samples					795:801	enriched samples	786:801	enriched samples	786:801	The highest content in reducing sugars was observed in enriched samples in comparison with high-fibre pasta.
26403803	3	5	from	present	619:625	arg1	pasta					710:714	the enriched pasta	697:714	the enriched pasta (samples D-F)	697:728	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	3	5	from	present	619:625	arg1	pasta					673:677	the commercially available high-fibre pasta	635:677	the commercially available high-fibre pasta (samples A-C)	635:691	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	3	5	from	present	619:625	arg1	A-C					688:690	samples A-C	680:690	samples A-C	680:690	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	3	5	from	present	619:625	arg1	D-F					725:727	samples D-F	717:727	samples D-F	717:727	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	1	6	contain	has	155:157	arg2	place					170:174	a definite place	159:174	a definite place	159:174	BACKGROUND Several studies have demonstrated that metabolomics has a definite place in food quality, nutritional value, and safety issues.
26403803	1	6	contain	has	155:157	arg1	metabolomics					142:153	metabolomics	142:153	metabolomics	142:153	BACKGROUND Several studies have demonstrated that metabolomics has a definite place in food quality, nutritional value, and safety issues.
26403803	2	7	theme	present	246:252	arg1	study					254:258	the present study	242:258	the present study	242:258	The aim of the present study was to determine and compare the metabolites in different pasta samples with fibre, and to investigate the modifications induced in these different kinds of pasta during cooking, using a gas chromatography-mass spectrometry-based metabolomics approach.
26403803	5	8	attach	presence	844:851	arg1	samples					872:878	samples	872:878	samples enriched with wheat bran	872:903	The presence of stigmasterol in samples enriched with wheat bran was relevant.
26403803	5	8	attach	presence	844:851	arg2	stigmasterol					856:867	stigmasterol	856:867	stigmasterol	856:867	The presence of stigmasterol in samples enriched with wheat bran was relevant.
26403803	3	9	theme	amino	559:563	arg1	acids					565:569	the amino acids	555:569	the amino acids	555:569	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	3	9	theme	amino	559:563	arg1	absent					583:588	absent	583:588	absent	583:588	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	9	10	theme	useful	1600:1605	arg1	tools					1607:1611	useful tools	1600:1611	useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta	1600:1702	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	6	11	theme	organic	1067:1073	arg1	acids					1075:1079	organic acids	1067:1079	organic acids	1067:1079	Cooking decreased all of the metabolites: the high-fibre pasta (A-C) and Control showed losses of amino acids and tocopherols, while for sugars and organic acids, the decrease depended on the pasta sample.
26403803	9	12	dep	CONCLUSIONS	1523:1533	arg1	established					1549:1559	established	1549:1559	has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking	1545:1733	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	1	13	theme	nutritional	193:203	arg1	value					205:209	nutritional value	193:209	nutritional value	193:209	BACKGROUND Several studies have demonstrated that metabolomics has a definite place in food quality, nutritional value, and safety issues.
26403803	7	14	theme	fatty	1271:1275	arg1	acids					1277:1281	saturated fatty acids	1261:1281	saturated fatty acids	1261:1281	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	3	15	theme	samples	717:723	arg1	pasta					710:714	the enriched pasta	697:714	the enriched pasta (samples D-F)	697:728	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	3	15	theme	samples	717:723	arg1	D-F					725:727	samples D-F	717:727	samples D-F	717:727	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	8	16	theme	component	1351:1359	arg1	analysis					1361:1368	Principal component analysis	1341:1368	Principal component analysis of the metabolites	1341:1387	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	7	17	with	pasta	1227:1231	arg1	barley					1238:1243	barley	1238:1243	barley	1238:1243	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	7	18	from	significant	1291:1301	arg1	pasta					1227:1231	pasta	1227:1231	pasta with barley	1227:1243	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	9	19	from	changes	1638:1644	arg1	compounds					1661:1669	nutritional compounds	1649:1669	nutritional compounds	1649:1669	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	9	19	from	changes	1638:1644	arg1	pasta					1698:1702	high-fibre and enriched pasta	1674:1702	high-fibre and enriched pasta	1674:1702	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	2	20	theme	spectrometry-based	471:488	arg1	approach					503:510	a gas chromatography-mass spectrometry-based metabolomics approach	445:510	a gas chromatography-mass spectrometry-based metabolomics approach	445:510	The aim of the present study was to determine and compare the metabolites in different pasta samples with fibre, and to investigate the modifications induced in these different kinds of pasta during cooking, using a gas chromatography-mass spectrometry-based metabolomics approach.
26403803	6	21	theme	pasta	1111:1115	arg1	sample					1117:1122	the pasta sample	1107:1122	the pasta sample	1107:1122	Cooking decreased all of the metabolites: the high-fibre pasta (A-C) and Control showed losses of amino acids and tocopherols, while for sugars and organic acids, the decrease depended on the pasta sample.
26403803	2	22	theme	gas	447:449	arg1	approach					503:510	a gas chromatography-mass spectrometry-based metabolomics approach	445:510	a gas chromatography-mass spectrometry-based metabolomics approach	445:510	The aim of the present study was to determine and compare the metabolites in different pasta samples with fibre, and to investigate the modifications induced in these different kinds of pasta during cooking, using a gas chromatography-mass spectrometry-based metabolomics approach.
26403803	8	23	theme	metabolites	1377:1387	arg1	analysis					1361:1368	Principal component analysis	1341:1368	Principal component analysis of the metabolites	1341:1387	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	8	23	theme	metabolites	1377:1387	arg1	effective					1422:1430	effective	1422:1430	effective	1422:1430	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	8	23	theme	metabolites	1377:1387	arg1	discrimination					1403:1416	the pasta discrimination	1393:1416	the pasta discrimination	1393:1416	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	7	24	theme	enriched	1129:1136	arg1	D-F					1153:1155	D-F	1153:1155	D-F	1153:1155	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	7	24	theme	enriched	1129:1136	arg1	samples					1144:1150	The enriched pasta samples	1125:1150	The enriched pasta samples (D-F)	1125:1156	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	5	25	from	presence	844:851	arg1	samples					872:878	samples	872:878	samples enriched with wheat bran	872:903	The presence of stigmasterol in samples enriched with wheat bran was relevant.
26403803	1	26	theme	definite	161:168	arg1	place					170:174	a definite place	159:174	a definite place	159:174	BACKGROUND Several studies have demonstrated that metabolomics has a definite place in food quality, nutritional value, and safety issues.
26403803	9	27	theme	high-fibre	1674:1683	arg1	pasta					1698:1702	high-fibre and enriched pasta	1674:1702	high-fibre and enriched pasta	1674:1702	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	9	28	from	tools	1607:1611	arg1	evaluation					1620:1629	the evaluation	1616:1629	the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta	1616:1702	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	9	29	theme	enriched	1689:1696	arg1	pasta					1698:1702	high-fibre and enriched pasta	1674:1702	high-fibre and enriched pasta	1674:1702	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	6	30	theme	high-fibre	965:974	arg1	A-C					983:985	A-C	983:985	A-C	983:985	Cooking decreased all of the metabolites: the high-fibre pasta (A-C) and Control showed losses of amino acids and tocopherols, while for sugars and organic acids, the decrease depended on the pasta sample.
26403803	6	30	theme	high-fibre	965:974	arg1	pasta					976:980	the high-fibre pasta	961:980	the high-fibre pasta (A-C)	961:986	Cooking decreased all of the metabolites: the high-fibre pasta (A-C) and Control showed losses of amino acids and tocopherols, while for sugars and organic acids, the decrease depended on the pasta sample.
26403803	2	31	theme	pasta	417:421	arg1	kinds					408:412	these different kinds	392:412	these different kinds of pasta	392:421	The aim of the present study was to determine and compare the metabolites in different pasta samples with fibre, and to investigate the modifications induced in these different kinds of pasta during cooking, using a gas chromatography-mass spectrometry-based metabolomics approach.
26403803	3	32	from	pasta	710:714	arg1	present					619:625	present	619:625	present	619:625	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	3	33	from	pasta	601:605	arg1	acids					565:569	the amino acids	555:569	the amino acids	555:569	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	3	33	from	pasta	601:605	arg1	absent					583:588	absent	583:588	absent	583:588	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	0	34	theme	Metabolomic	0:10	arg1	analysis					12:19	Metabolomic analysis	0:19	Metabolomic analysis	0:19	Metabolomic analysis can detect the composition of pasta enriched with fibre after cooking.
26403803	5	35	theme	wheat	894:898	arg1	bran					900:903	wheat bran	894:903	wheat bran	894:903	The presence of stigmasterol in samples enriched with wheat bran was relevant.
26403803	3	36	dep	pasta	673:677	arg1	both					627:630	both	627:630	both	627:630	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	1	37	theme	Several	103:109	arg1	studies					111:117	BACKGROUND Several studies	92:117	BACKGROUND Several studies	92:117	BACKGROUND Several studies have demonstrated that metabolomics has a definite place in food quality, nutritional value, and safety issues.
26403803	7	38	theme	phytosterols	1206:1217	arg1	exception					1193:1201	the exception	1189:1201	the exception of phytosterols	1189:1217	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	8	39	theme	enriched	1455:1462	arg1	pasta					1464:1468	the enriched pasta	1451:1468	the enriched pasta from the commercial pasta, both uncooked and cooked	1451:1520	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	2	40	theme	chromatography-mass	451:469	arg1	approach					503:510	a gas chromatography-mass spectrometry-based metabolomics approach	445:510	a gas chromatography-mass spectrometry-based metabolomics approach	445:510	The aim of the present study was to determine and compare the metabolites in different pasta samples with fibre, and to investigate the modifications induced in these different kinds of pasta during cooking, using a gas chromatography-mass spectrometry-based metabolomics approach.
26403803	3	41	theme	samples	680:686	arg1	pasta					673:677	the commercially available high-fibre pasta	635:677	the commercially available high-fibre pasta (samples A-C)	635:691	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	3	41	theme	samples	680:686	arg1	A-C					688:690	samples A-C	680:690	samples A-C	680:690	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	2	42	theme	pasta	318:322	arg1	samples					324:330	different pasta samples	308:330	different pasta samples	308:330	The aim of the present study was to determine and compare the metabolites in different pasta samples with fibre, and to investigate the modifications induced in these different kinds of pasta during cooking, using a gas chromatography-mass spectrometry-based metabolomics approach.
26403803	7	43	with	pasta	1325:1329	arg1	oat					1336:1338	oat	1336:1338	oat	1336:1338	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	9	44	from	compounds	1661:1669	arg1	evaluation					1620:1629	the evaluation	1616:1629	the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta	1616:1702	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	8	45	theme	commercial	1479:1488	arg1	pasta					1490:1494	the commercial pasta	1475:1494	the commercial pasta	1475:1494	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	4	46	theme	highest	735:741	arg1	content					743:749	The highest content	731:749	The highest content in reducing sugars	731:768	The highest content in reducing sugars was observed in enriched samples in comparison with high-fibre pasta.
26403803	7	47	theme	same	1169:1172	arg1	decreases					1174:1182	the same decreases	1165:1182	the same decreases	1165:1182	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	8	48	theme	uncooked	1502:1509	arg1	pasta					1490:1494	the commercial pasta	1475:1494	the commercial pasta	1475:1494	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	6	49	theme	amino	1017:1021	arg1	acids					1023:1027	amino acids	1017:1027	amino acids	1017:1027	Cooking decreased all of the metabolites: the high-fibre pasta (A-C) and Control showed losses of amino acids and tocopherols, while for sugars and organic acids, the decrease depended on the pasta sample.
26403803	8	50	theme	cooked	1515:1520	arg1	pasta					1490:1494	the commercial pasta	1475:1494	the commercial pasta	1475:1494	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	3	51	theme	high-fibre	662:671	arg1	pasta					673:677	the commercially available high-fibre pasta	635:677	the commercially available high-fibre pasta (samples A-C)	635:691	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	3	51	theme	high-fibre	662:671	arg1	A-C					688:690	samples A-C	680:690	samples A-C	680:690	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	4	52	theme	high-fibre	822:831	arg1	pasta					833:837	high-fibre pasta	822:837	high-fibre pasta	822:837	The highest content in reducing sugars was observed in enriched samples in comparison with high-fibre pasta.
26403803	8	53	from	pasta	1490:1494	arg1	pasta					1464:1468	the enriched pasta	1451:1468	the enriched pasta from the commercial pasta, both uncooked and cooked	1451:1520	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	2	54	theme	study	254:258	arg1	aim					235:237	The aim	231:237	The aim of the present study	231:258	The aim of the present study was to determine and compare the metabolites in different pasta samples with fibre, and to investigate the modifications induced in these different kinds of pasta during cooking, using a gas chromatography-mass spectrometry-based metabolomics approach.
26403803	9	55	theme	metabolomic	1571:1581	arg1	analyses					1583:1590	such metabolomic analyses	1566:1590	such metabolomic analyses	1566:1590	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	1	56	theme	food	179:182	arg1	quality					184:190	food quality	179:190	food quality	179:190	BACKGROUND Several studies have demonstrated that metabolomics has a definite place in food quality, nutritional value, and safety issues.
26403803	8	57	theme	pasta	1397:1401	arg1	effective					1422:1430	effective	1422:1430	effective	1422:1430	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	8	57	theme	pasta	1397:1401	arg1	discrimination					1403:1416	the pasta discrimination	1393:1416	the pasta discrimination	1393:1416	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	3	58	theme	enriched	701:708	arg1	pasta					710:714	the enriched pasta	697:714	the enriched pasta (samples D-F)	697:728	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	3	58	theme	enriched	701:708	arg1	D-F					725:727	samples D-F	717:727	samples D-F	717:727	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	8	59	theme	Principal	1341:1349	arg1	analysis					1361:1368	Principal component analysis	1341:1368	Principal component analysis of the metabolites	1341:1387	Principal component analysis of the metabolites and the pasta discrimination was effective in differentiating the enriched pasta from the commercial pasta, both uncooked and cooked.
26403803	7	60	theme	acids	1277:1281	arg1	significant					1291:1301	significant	1291:1301	significant	1291:1301	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	7	60	theme	acids	1277:1281	arg1	decrease					1249:1256	the decrease	1245:1256	the decrease of saturated fatty acids	1245:1281	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	3	61	from	pasta	673:677	arg1	present					619:625	present	619:625	present	619:625	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	5	62	theme	stigmasterol	856:867	arg1	presence					844:851	The presence	840:851	The presence of stigmasterol in samples enriched with wheat bran	840:903	The presence of stigmasterol in samples enriched with wheat bran was relevant.
26403803	5	62	theme	stigmasterol	856:867	arg1	relevant					909:916	relevant	909:916	relevant	909:916	The presence of stigmasterol in samples enriched with wheat bran was relevant.
26403803	7	63	theme	saturated	1261:1269	arg1	acids					1277:1281	saturated fatty acids	1261:1281	saturated fatty acids	1261:1281	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	3	64	theme	control	593:599	arg1	pasta					601:605	control pasta	593:605	control pasta	593:605	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	9	65	theme	changes	1638:1644	arg1	evaluation					1620:1629	the evaluation	1616:1629	the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta	1616:1702	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	7	66	theme	pasta	1138:1142	arg1	D-F					1153:1155	D-F	1153:1155	D-F	1153:1155	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	7	66	theme	pasta	1138:1142	arg1	samples					1144:1150	The enriched pasta samples	1125:1150	The enriched pasta samples (D-F)	1125:1156	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	4	67	from	content	743:749	arg1	sugars					763:768	reducing sugars	754:768	reducing sugars	754:768	The highest content in reducing sugars was observed in enriched samples in comparison with high-fibre pasta.
26403803	4	68	with	comparison	806:815	arg1	pasta					833:837	high-fibre pasta	822:837	high-fibre pasta	822:837	The highest content in reducing sugars was observed in enriched samples in comparison with high-fibre pasta.
26403803	9	69	from	evaluation	1620:1629	arg1	compounds					1661:1669	nutritional compounds	1649:1669	nutritional compounds	1649:1669	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	9	69	from	evaluation	1620:1629	arg1	pasta					1698:1702	high-fibre and enriched pasta	1674:1702	high-fibre and enriched pasta	1674:1702	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	9	70	theme	nutritional	1649:1659	arg1	compounds					1661:1669	nutritional compounds	1649:1669	nutritional compounds	1649:1669	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	9	71	theme	such	1566:1569	arg1	analyses					1583:1590	such metabolomic analyses	1566:1590	such metabolomic analyses	1566:1590	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	7	72	from	tocopherols	1310:1320	arg1	pasta					1325:1329	pasta	1325:1329	pasta with oat	1325:1338	The enriched pasta samples (D-F) showed the same decreases with the exception of phytosterols, and in pasta with barley the decrease of saturated fatty acids was not significant as for tocopherols in pasta with oat.
26403803	6	73	dep	metabolites	948:958	arg1	showed					1000:1005	showed	1000:1005	showed	1000:1005	Cooking decreased all of the metabolites: the high-fibre pasta (A-C) and Control showed losses of amino acids and tocopherols, while for sugars and organic acids, the decrease depended on the pasta sample.
26403803	3	74	from	absent	583:588	arg1	pasta					601:605	control pasta	593:605	control pasta	593:605	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
26403803	2	75	theme	metabolomics	490:501	arg1	approach					503:510	a gas chromatography-mass spectrometry-based metabolomics approach	445:510	a gas chromatography-mass spectrometry-based metabolomics approach	445:510	The aim of the present study was to determine and compare the metabolites in different pasta samples with fibre, and to investigate the modifications induced in these different kinds of pasta during cooking, using a gas chromatography-mass spectrometry-based metabolomics approach.
26403803	1	76	theme	BACKGROUND	92:101	arg1	studies					111:117	BACKGROUND Several studies	92:117	BACKGROUND Several studies	92:117	BACKGROUND Several studies have demonstrated that metabolomics has a definite place in food quality, nutritional value, and safety issues.
26403803	2	77	theme	different	398:406	arg1	kinds					408:412	these different kinds	392:412	these different kinds of pasta	392:421	The aim of the present study was to determine and compare the metabolites in different pasta samples with fibre, and to investigate the modifications induced in these different kinds of pasta during cooking, using a gas chromatography-mass spectrometry-based metabolomics approach.
26403803	10	78	theme	Chemical	1754:1761	arg1	Industry					1763:1770	Chemical Industry	1754:1770	Chemical Industry	1754:1770	© 2015 Society of Chemical Industry.
26403803	9	79	from	pasta	1698:1702	arg1	evaluation					1620:1629	the evaluation	1616:1629	the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta	1616:1702	CONCLUSIONS The study has established that such metabolomic analyses provide useful tools in the evaluation of the changes in nutritional compounds in high-fibre and enriched pasta, both before and after cooking.
26403803	4	80	theme	reducing	754:761	arg1	sugars					763:768	reducing sugars	754:768	reducing sugars	754:768	The highest content in reducing sugars was observed in enriched samples in comparison with high-fibre pasta.
26403803	0	81	theme	pasta	51:55	arg1	composition					36:46	the composition	32:46	the composition of pasta enriched with fibre after cooking	32:89	Metabolomic analysis can detect the composition of pasta enriched with fibre after cooking.
26403803	2	82	theme	different	308:316	arg1	samples					324:330	different pasta samples	308:330	different pasta samples	308:330	The aim of the present study was to determine and compare the metabolites in different pasta samples with fibre, and to investigate the modifications induced in these different kinds of pasta during cooking, using a gas chromatography-mass spectrometry-based metabolomics approach.
26403803	1	83	theme	safety	216:221	arg1	issues					223:228	safety issues	216:228	safety issues	216:228	BACKGROUND Several studies have demonstrated that metabolomics has a definite place in food quality, nutritional value, and safety issues.
26403803	6	84	theme	tocopherols	1033:1043	arg1	losses					1007:1012	losses	1007:1012	losses of amino acids and tocopherols	1007:1043	Cooking decreased all of the metabolites: the high-fibre pasta (A-C) and Control showed losses of amino acids and tocopherols, while for sugars and organic acids, the decrease depended on the pasta sample.
26403803	4	85	located	observed	774:781	arg1	samples					795:801	enriched samples	786:801	enriched samples	786:801	The highest content in reducing sugars was observed in enriched samples in comparison with high-fibre pasta.
26403803	4	85	located	observed	774:781	arg1	comparison					806:815	comparison	806:815	comparison with high-fibre pasta	806:837	The highest content in reducing sugars was observed in enriched samples in comparison with high-fibre pasta.
26403803	4	85	located	observed	774:781	arg2	content					743:749	The highest content	731:749	The highest content in reducing sugars	731:768	The highest content in reducing sugars was observed in enriched samples in comparison with high-fibre pasta.
26403803	3	86	theme	RESULTS	513:519	arg1	Differences					521:531	RESULTS Differences	513:531	RESULTS Differences	513:531	RESULTS Differences were seen for some of the amino acids, which were absent in control pasta, while were present both in the commercially available high-fibre pasta (samples A-C) and the enriched pasta (samples D-F).
29083485	0	0	theme	Oil/β-Cyclodextrin	78:95	arg1	Complex					107:113	Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex	57:113	Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex	57:113	Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex.
29083485	6	1	theme	complexes	1284:1292	arg1	characteristics					1251:1265	the characteristics	1247:1265	the characteristics of the inclusion complexes	1247:1292	Therefore, the results showed that the chemical composition, molecular size, and structure of the essential oils influence the characteristics of the inclusion complexes.
29083485	7	2	theme	complexes	1353:1361	arg1	formation					1330:1338	the formation	1326:1338	the formation of inclusion complexes with essential oils	1326:1381	The application of the β-CD in the formation of inclusion complexes with essential oils can expand the potential applications in foods.
29083485	1	3	theme	orange	229:234	arg1	OEO					251:253	OEO	251:253	OEO	251:253	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	1	3	theme	orange	229:234	arg1	oil					246:248	orange essential oil	229:248	orange essential oil (OEO)	229:254	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	2	4	theme	thermal	425:431	arg1	stability					433:441	thermal stability	425:441	thermal stability	425:441	The samples were evaluated by chemical composition, morphology, thermal stability, and volatile compounds by static headspace-gas chromatography (SH-GC).
29083485	6	5	theme	molecular	1185:1193	arg1	size					1195:1198	molecular size	1185:1198	molecular size	1185:1198	Therefore, the results showed that the chemical composition, molecular size, and structure of the essential oils influence the characteristics of the inclusion complexes.
29083485	7	6	theme	potential	1398:1406	arg1	applications					1408:1419	the potential applications	1394:1419	the potential applications in foods	1394:1428	The application of the β-CD in the formation of inclusion complexes with essential oils can expand the potential applications in foods.
29083485	1	7	theme	essential	236:244	arg1	OEO					251:253	OEO	251:253	OEO	251:253	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	1	7	theme	essential	236:244	arg1	oil					246:248	orange essential oil	229:248	orange essential oil (OEO)	229:254	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	0	8	theme	Complex	107:113	arg1	Characterization					12:27	Characterization	12:27	Characterization	12:27	Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex.
29083485	0	8	theme	Complex	107:113	arg1	Stability					34:42	Stability	34:42	Stability	34:42	Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex.
29083485	0	8	theme	Complex	107:113	arg1	Production					0:9	Production	0:9	Production	0:9	Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex.
29083485	4	9	theme	method	864:869	arg1	necessity					833:841	the necessity	829:841	the necessity of the precipitation method used to guarantee the interaction between β-CD and essential oils	829:935	These differences show that there was the formation of the inclusion complex and demonstrate the necessity of the precipitation method used to guarantee the interaction between β-CD and essential oils.
29083485	4	10	theme	inclusion	795:803	arg1	complex					805:811	the inclusion complex	791:811	the inclusion complex	791:811	These differences show that there was the formation of the inclusion complex and demonstrate the necessity of the precipitation method used to guarantee the interaction between β-CD and essential oils.
29083485	3	11	dep	oils	615:618	arg1	EEO					629:631	EEO	629:631	EEO	629:631	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	3	11	dep	oils	615:618	arg1	OEO					621:623	OEO	621:623	OEO	621:623	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	3	11	dep	oils	615:618	arg1	oils					615:618	both essential oils	600:618	both essential oils (OEO and EEO)	600:632	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	0	12	theme	Inclusion	97:105	arg1	Complex					107:113	Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex	57:113	Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex	57:113	Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex.
29083485	7	13	theme	essential	1368:1376	arg1	oils					1378:1381	essential oils	1368:1381	essential oils	1368:1381	The application of the β-CD in the formation of inclusion complexes with essential oils can expand the potential applications in foods.
29083485	6	14	theme	chemical	1163:1170	arg1	composition					1172:1182	chemical composition	1163:1182	chemical composition	1163:1182	Therefore, the results showed that the chemical composition, molecular size, and structure of the essential oils influence the characteristics of the inclusion complexes.
29083485	2	15	theme	volatile	448:455	arg1	compounds					457:465	volatile compounds	448:465	volatile compounds	448:465	The samples were evaluated by chemical composition, morphology, thermal stability, and volatile compounds by static headspace-gas chromatography (SH-GC).
29083485	7	16	theme	inclusion	1343:1351	arg1	complexes					1353:1361	inclusion complexes	1343:1361	inclusion complexes with essential oils	1343:1381	The application of the β-CD in the formation of inclusion complexes with essential oils can expand the potential applications in foods.
29083485	5	17	theme	free	1103:1106	arg1	oils					1118:1121	free essential oils	1103:1121	free essential oils	1103:1121	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils.
29083485	1	18	theme	inclusion	170:178	arg1	complexes					180:188	inclusion complexes	170:188	inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO)	170:288	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	1	18	theme	inclusion	170:178	arg1	IC					191:192	IC	191:192	IC	191:192	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	2	19	theme	chemical	391:398	arg1	composition					400:410	chemical composition	391:410	chemical composition	391:410	The samples were evaluated by chemical composition, morphology, thermal stability, and volatile compounds by static headspace-gas chromatography (SH-GC).
29083485	0	20	theme	Essential	68:76	arg1	Complex					107:113	Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex	57:113	Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex	57:113	Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex.
29083485	6	21	theme	essential	1222:1230	arg1	oils					1232:1235	the essential oils	1218:1235	the essential oils	1218:1235	Therefore, the results showed that the chemical composition, molecular size, and structure of the essential oils influence the characteristics of the inclusion complexes.
29083485	7	22	theme	β-CD	1318:1321	arg1	application					1299:1309	The application	1295:1309	The application of the β-CD in the formation of inclusion complexes with essential oils	1295:1381	The application of the β-CD in the formation of inclusion complexes with essential oils can expand the potential applications in foods.
29083485	5	23	from	loss	947:950	arg1	oils					998:1001	both essential oils	983:1001	both essential oils	983:1001	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils.
29083485	5	24	theme	physical	1081:1088	arg1	mixtures					1090:1097	their physical mixtures	1075:1097	their physical mixtures	1075:1097	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils.
29083485	6	25	theme	inclusion	1274:1282	arg1	complexes					1284:1292	the inclusion complexes	1270:1292	the inclusion complexes	1270:1292	Therefore, the results showed that the chemical composition, molecular size, and structure of the essential oils influence the characteristics of the inclusion complexes.
29083485	6	26	theme	oils	1232:1235	arg1	structure					1205:1213	structure	1205:1213	structure	1205:1213	Therefore, the results showed that the chemical composition, molecular size, and structure of the essential oils influence the characteristics of the inclusion complexes.
29083485	6	26	theme	oils	1232:1235	arg1	composition					1172:1182	chemical composition	1163:1182	chemical composition	1163:1182	Therefore, the results showed that the chemical composition, molecular size, and structure of the essential oils influence the characteristics of the inclusion complexes.
29083485	6	26	theme	oils	1232:1235	arg1	size					1195:1198	molecular size	1185:1198	molecular size	1185:1198	Therefore, the results showed that the chemical composition, molecular size, and structure of the essential oils influence the characteristics of the inclusion complexes.
29083485	1	27	theme	pure	323:326	arg1	compounds					328:336	their pure compounds	317:336	their pure compounds	317:336	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	4	28	theme	essential	922:930	arg1	oils					932:935	essential oils	922:935	essential oils	922:935	These differences show that there was the formation of the inclusion complex and demonstrate the necessity of the precipitation method used to guarantee the interaction between β-CD and essential oils.
29083485	1	29	theme	eucalyptus	259:268	arg1	oil					280:282	eucalyptus essential oil	259:282	eucalyptus essential oil (EEO)	259:288	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	1	29	theme	eucalyptus	259:268	arg1	EEO					285:287	EEO	285:287	EEO	285:287	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	4	30	theme	precipitation	850:862	arg1	method					864:869	the precipitation method	846:869	the precipitation method used to guarantee the interaction between β-CD and essential oils	846:935	These differences show that there was the formation of the inclusion complex and demonstrate the necessity of the precipitation method used to guarantee the interaction between β-CD and essential oils.
29083485	5	31	theme	essential	988:996	arg1	oils					998:1001	both essential oils	983:1001	both essential oils	983:1001	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils.
29083485	5	32	theme	essential	1108:1116	arg1	oils					1118:1121	free essential oils	1103:1121	free essential oils	1103:1121	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils.
29083485	1	33	theme	essential	270:278	arg1	oil					280:282	eucalyptus essential oil	259:282	eucalyptus essential oil (EEO)	259:288	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	1	33	theme	essential	270:278	arg1	EEO					285:287	EEO	285:287	EEO	285:287	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	2	34	theme	headspace-gas	477:489	arg1	SH-GC					507:511	SH-GC	507:511	SH-GC	507:511	The samples were evaluated by chemical composition, morphology, thermal stability, and volatile compounds by static headspace-gas chromatography (SH-GC).
29083485	2	34	theme	headspace-gas	477:489	arg1	chromatography					491:504	static headspace-gas chromatography	470:504	static headspace-gas chromatography (SH-GC)	470:512	The samples were evaluated by chemical composition, morphology, thermal stability, and volatile compounds by static headspace-gas chromatography (SH-GC).
29083485	3	35	theme	inclusion	578:586	arg1	complex					588:594	the inclusion complex	574:594	the inclusion complex	574:594	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	5	36	theme	slow	942:945	arg1	loss					947:950	The slow loss	938:950	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD,	938:1028	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils.
29083485	0	37	theme	Orange	47:52	arg1	Characterization					12:27	Characterization	12:27	Characterization	12:27	Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex.
29083485	0	37	theme	Orange	47:52	arg1	Stability					34:42	Stability	34:42	Stability	34:42	Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex.
29083485	0	37	theme	Orange	47:52	arg1	Production					0:9	Production	0:9	Production	0:9	Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex.
29083485	2	38	theme	static	470:475	arg1	SH-GC					507:511	SH-GC	507:511	SH-GC	507:511	The samples were evaluated by chemical composition, morphology, thermal stability, and volatile compounds by static headspace-gas chromatography (SH-GC).
29083485	2	38	theme	static	470:475	arg1	chromatography					491:504	static headspace-gas chromatography	470:504	static headspace-gas chromatography (SH-GC)	470:512	The samples were evaluated by chemical composition, morphology, thermal stability, and volatile compounds by static headspace-gas chromatography (SH-GC).
29083485	5	39	from	oils	998:1001	arg1	compounds					968:976	the volatile compounds	955:976	the volatile compounds from both essential oils, when complexed with β-CD,	955:1028	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils.
29083485	5	39	from	oils	998:1001	arg1	loss					947:950	The slow loss	938:950	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD,	938:1028	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils.
29083485	1	40	theme	physical	342:349	arg1	mixtures					351:358	physical mixtures	342:358	physical mixtures	342:358	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	3	41	theme	free	529:532	arg1	oil					544:546	the free essential oil	525:546	the free essential oil	525:546	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	5	42	theme	higher	1039:1044	arg1	stability					1046:1054	a higher stability	1037:1054	a higher stability	1037:1054	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils.
29083485	3	43	theme	chemical	679:686	arg1	composition					688:698	the chemical composition	675:698	the chemical composition	675:698	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	4	44	theme	complex	805:811	arg1	formation					778:786	the formation	774:786	the formation of the inclusion complex	774:811	These differences show that there was the formation of the inclusion complex and demonstrate the necessity of the precipitation method used to guarantee the interaction between β-CD and essential oils.
29083485	3	45	theme	essential	534:542	arg1	oil					544:546	the free essential oil	525:546	the free essential oil	525:546	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	7	46	from	application	1299:1309	arg1	formation					1330:1338	the formation	1326:1338	the formation of inclusion complexes with essential oils	1326:1381	The application of the β-CD in the formation of inclusion complexes with essential oils can expand the potential applications in foods.
29083485	3	47	dep	observed	642:649	arg1	Comparing					515:523	Comparing	515:523	Comparing the free essential oil and physical mixture with the inclusion complex	515:594	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	5	48	theme	volatile	959:966	arg1	compounds					968:976	the volatile compounds	955:976	the volatile compounds from both essential oils, when complexed with β-CD,	955:1028	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils.
29083485	7	49	with	complexes	1353:1361	arg1	oils					1378:1381	essential oils	1368:1381	essential oils	1368:1381	The application of the β-CD in the formation of inclusion complexes with essential oils can expand the potential applications in foods.
29083485	5	50	theme	compounds	968:976	arg1	loss					947:950	The slow loss	938:950	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD,	938:1028	The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils.
29083485	1	51	theme	study	132:136	arg1	aim					120:122	The aim	116:122	The aim of this study	116:136	The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures.
29083485	0	52	theme	Eucalyptus	57:66	arg1	Complex					107:113	Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex	57:113	Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex	57:113	Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex.
29083485	3	53	theme	essential	605:613	arg1	EEO					629:631	EEO	629:631	EEO	629:631	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	3	53	theme	essential	605:613	arg1	OEO					621:623	OEO	621:623	OEO	621:623	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	3	53	theme	essential	605:613	arg1	oils					615:618	both essential oils	600:618	both essential oils (OEO and EEO)	600:632	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	3	54	theme	physical	552:559	arg1	mixture					561:567	physical mixture	552:567	physical mixture	552:567	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
29083485	7	55	from	applications	1408:1419	arg1	foods					1424:1428	foods	1424:1428	foods	1424:1428	The application of the β-CD in the formation of inclusion complexes with essential oils can expand the potential applications in foods.
29083485	3	56	theme	thermal	701:707	arg1	stability					709:717	thermal stability	701:717	thermal stability	701:717	Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology.
26398200	0	0	theme	Cooling	78:84	arg1	Rate					86:89	Cooling Rate	78:89	Cooling Rate	78:89	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.
26398200	5	1	theme	crystallized	1123:1134	arg1	dihydrate					1146:1154	crystallized trehalose dihydrate	1123:1154	crystallized trehalose dihydrate	1123:1154	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	4	2	theme	higher	866:871	arg1	amount					873:878	a relatively higher amount	853:878	a relatively higher amount of cumulative aggregation	853:904	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	2	theme	higher	866:871	arg1	aggregation					894:904	cumulative aggregation	883:904	cumulative aggregation	883:904	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	1	3	from	effects	155:161	arg1	stability					273:281	protein stability	265:281	protein stability	265:281	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	1	3	from	effects	155:161	arg1	distribution					248:259	trehalose phase distribution	232:259	trehalose phase distribution	232:259	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	4	4	dep	higher	790:795	arg1	-8°C					798:801	-8°C	798:801	-8°C	798:801	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	6	5	theme	capable	1295:1301	arg1	ratio					1279:1283	trehalose-mAb (w/w) ratio	1259:1283	trehalose-mAb (w/w) ratio	1259:1283	The results identify an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations.
26398200	5	6	theme	trehalose	1200:1208	arg1	ratio					1191:1195	the ratio	1187:1195	the ratio of trehalose to mAb	1187:1215	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	1	7	from	distribution	248:259	arg1	solutions					293:301	frozen solutions	286:301	frozen solutions	286:301	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	0	8	from	Aggregation	8:18	arg1	Formulations					40:51	Frozen Trehalose Formulations	23:51	Frozen Trehalose Formulations	23:51	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.
26398200	2	9	theme	cooling	337:343	arg1	rates					345:349	faster cooling rates	330:349	faster cooling rates (>100°C/min)	330:362	The data demonstrate that faster cooling rates (>100°C/min) result in trehalose crystallization and protein aggregation as determined by Fourier Transform Near-Infrared (FT-NIR) spectroscopy and size-exclusion chromatography, respectively.
26398200	2	9	theme	cooling	337:343	arg1	>100°C/min					352:361	>100°C/min	352:361	>100°C/min	352:361	The data demonstrate that faster cooling rates (>100°C/min) result in trehalose crystallization and protein aggregation as determined by Fourier Transform Near-Infrared (FT-NIR) spectroscopy and size-exclusion chromatography, respectively.
26398200	1	10	theme	trehalose	232:240	arg1	distribution					248:259	trehalose phase distribution	232:259	trehalose phase distribution	232:259	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	6	11	theme	frozen	1363:1368	arg1	storage-even					1370:1381	long-term frozen storage-even	1353:1381	long-term frozen storage-even for fast cooled (>100°C/min) formulations	1353:1423	The results identify an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations.
26398200	5	12	theme	dihydrate	1146:1154	arg1	distribution					1093:1104	the phase distribution	1083:1104	the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions	1083:1174	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	6	13	theme	w/w	1274:1276	arg1	ratio					1279:1283	trehalose-mAb (w/w) ratio	1259:1283	trehalose-mAb (w/w) ratio	1259:1283	The results identify an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations.
26398200	4	14	theme	higher	790:795	arg1	temperatures					830:841	higher (-8°C) and lower (-20°C) storage temperatures	790:841	higher (-8°C) and lower (-20°C) storage temperatures	790:841	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	1	15	theme	phase	242:246	arg1	distribution					248:259	trehalose phase distribution	232:259	trehalose phase distribution	232:259	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	0	16	theme	Rate	86:89	arg1	Effects					54:60	Effects	54:60	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.	0:115	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.
26398200	5	17	theme	phase	1087:1091	arg1	distribution					1093:1104	the phase distribution	1083:1104	the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions	1083:1174	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	3	18	theme	slower	559:564	arg1	rates					574:578	slower cooling rates	559:578	slower cooling rates (≤1°C/min)	559:589	Conversely, at slower cooling rates (≤1°C/min), trehalose remains predominantly amorphous and there is no effect on protein stability.
26398200	3	18	theme	slower	559:564	arg1	≤1°C/min					581:588	≤1°C/min	581:588	≤1°C/min	581:588	Conversely, at slower cooling rates (≤1°C/min), trehalose remains predominantly amorphous and there is no effect on protein stability.
26398200	6	19	theme	mAb	1329:1331	arg1	formulations					1333:1344	mAb formulations	1329:1344	mAb formulations	1329:1344	The results identify an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations.
26398200	0	20	theme	Storage	96:102	arg1	Temperature					104:114	Storage Temperature	96:114	Storage Temperature	96:114	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.
26398200	1	21	from	stability	273:281	arg1	solutions					293:301	frozen solutions	286:301	frozen solutions	286:301	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	2	22	theme	trehalose	374:382	arg1	crystallization					384:398	trehalose crystallization	374:398	trehalose crystallization	374:398	The data demonstrate that faster cooling rates (>100°C/min) result in trehalose crystallization and protein aggregation as determined by Fourier Transform Near-Infrared (FT-NIR) spectroscopy and size-exclusion chromatography, respectively.
26398200	3	23	from	effect	650:655	arg1	stability					668:676	protein stability	660:676	protein stability	660:676	Conversely, at slower cooling rates (≤1°C/min), trehalose remains predominantly amorphous and there is no effect on protein stability.
26398200	4	24	theme	storage	969:975	arg1	-8°C					1001:1004	-8°C	1001:1004	-8°C	1001:1004	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	24	theme	storage	969:975	arg1	-14°C					991:995	-14°C	991:995	-14°C	991:995	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	24	theme	storage	969:975	arg1	temperatures					977:988	higher storage temperatures	962:988	higher storage temperatures (-14°C and -8°C)	962:1005	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	5	25	theme	amorphous	1109:1117	arg1	distribution					1093:1104	the phase distribution	1083:1104	the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions	1083:1174	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	2	26	dep	Fourier	441:447	arg1	Transform					449:457	Transform	449:457	Transform	449:457	The data demonstrate that faster cooling rates (>100°C/min) result in trehalose crystallization and protein aggregation as determined by Fourier Transform Near-Infrared (FT-NIR) spectroscopy and size-exclusion chromatography, respectively.
26398200	2	27	theme	size-exclusion	499:512	arg1	chromatography					514:527	size-exclusion chromatography	499:527	size-exclusion chromatography	499:527	The data demonstrate that faster cooling rates (>100°C/min) result in trehalose crystallization and protein aggregation as determined by Fourier Transform Near-Infrared (FT-NIR) spectroscopy and size-exclusion chromatography, respectively.
26398200	4	28	dep	temperatures	977:988	arg1	-8°C					1001:1004	-8°C	1001:1004	-8°C	1001:1004	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	28	dep	temperatures	977:988	arg1	-14°C					991:995	-14°C	991:995	-14°C	991:995	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	28	dep	temperatures	977:988	arg1	temperatures					977:988	higher storage temperatures	962:988	higher storage temperatures (-14°C and -8°C)	962:1005	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	0	29	theme	Protein	0:6	arg1	Aggregation					8:18	Protein Aggregation	0:18	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.	0:115	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.
26398200	6	30	theme	optimal	1242:1248	arg1	range					1250:1254	an optimal range	1239:1254	an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations	1239:1423	The results identify an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations.
26398200	6	31	theme	trehalose-mAb	1259:1271	arg1	ratio					1279:1283	trehalose-mAb (w/w) ratio	1259:1283	trehalose-mAb (w/w) ratio	1259:1283	The results identify an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations.
26398200	5	32	theme	trehalose	1136:1144	arg1	dihydrate					1146:1154	crystallized trehalose dihydrate	1123:1154	crystallized trehalose dihydrate	1123:1154	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	1	33	theme	cooling	166:172	arg1	rate					174:177	cooling rate	166:177	cooling rate	166:177	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	0	34	theme	Frozen	23:28	arg1	Formulations					40:51	Frozen Trehalose Formulations	23:51	Frozen Trehalose Formulations	23:51	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.
26398200	1	35	theme	protein	265:271	arg1	stability					273:281	protein stability	265:281	protein stability	265:281	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	4	36	theme	lower	922:926	arg1	temperature					936:946	lower (-20°C) temperature	922:946	lower (-20°C) temperature	922:946	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	3	37	theme	cooling	566:572	arg1	rates					574:578	slower cooling rates	559:578	slower cooling rates (≤1°C/min)	559:589	Conversely, at slower cooling rates (≤1°C/min), trehalose remains predominantly amorphous and there is no effect on protein stability.
26398200	3	37	theme	cooling	566:572	arg1	≤1°C/min					581:588	≤1°C/min	581:588	≤1°C/min	581:588	Conversely, at slower cooling rates (≤1°C/min), trehalose remains predominantly amorphous and there is no effect on protein stability.
26398200	1	38	theme	rate	174:177	arg1	effects					155:161	the effects	151:161	the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions	151:301	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	5	39	from	distribution	1093:1104	arg1	solutions					1166:1174	frozen solutions	1159:1174	frozen solutions	1159:1174	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	0	40	theme	Temperature	104:114	arg1	Effects					54:60	Effects	54:60	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.	0:115	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.
26398200	5	41	theme	composition	1057:1067	arg1	effects					1034:1040	the effects	1030:1040	the effects of formulation composition	1030:1067	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	4	42	theme	temperatures	701:712	arg1	Evaluation					679:688	Evaluation	679:688	Evaluation of storage temperatures	679:712	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	2	43	theme	protein	404:410	arg1	aggregation					412:422	protein aggregation	404:422	protein aggregation	404:422	The data demonstrate that faster cooling rates (>100°C/min) result in trehalose crystallization and protein aggregation as determined by Fourier Transform Near-Infrared (FT-NIR) spectroscopy and size-exclusion chromatography, respectively.
26398200	2	44	theme	FT-NIR	474:479	arg1	spectroscopy					482:493	Near-Infrared (FT-NIR) spectroscopy	459:493	Near-Infrared (FT-NIR) spectroscopy	459:493	The data demonstrate that faster cooling rates (>100°C/min) result in trehalose crystallization and protein aggregation as determined by Fourier Transform Near-Infrared (FT-NIR) spectroscopy and size-exclusion chromatography, respectively.
26398200	6	45	theme	ratio	1279:1283	arg1	range					1250:1254	an optimal range	1239:1254	an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations	1239:1423	The results identify an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations.
26398200	4	46	located	observed	910:917	arg2	amount					873:878	a relatively higher amount	853:878	a relatively higher amount of cumulative aggregation	853:904	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	46	located	observed	910:917	arg1	temperature					936:946	lower (-20°C) temperature	922:946	lower (-20°C) temperature	922:946	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	46	located	observed	910:917	arg2	aggregation					894:904	cumulative aggregation	883:904	cumulative aggregation	883:904	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	1	47	theme	storage	180:186	arg1	temperature					188:198	storage temperature	180:198	storage temperature	180:198	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	0	48	theme	Trehalose	30:38	arg1	Formulations					40:51	Frozen Trehalose Formulations	23:51	Frozen Trehalose Formulations	23:51	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.
26398200	1	49	theme	frozen	286:291	arg1	solutions					293:301	frozen solutions	286:301	frozen solutions	286:301	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	4	50	dep	lower	922:926	arg1	-20°C					929:933	-20°C	929:933	-20°C	929:933	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	1	51	theme	temperature	188:198	arg1	effects					155:161	the effects	151:161	the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions	151:301	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	2	52	theme	Near-Infrared	459:471	arg1	spectroscopy					482:493	Near-Infrared (FT-NIR) spectroscopy	459:493	Near-Infrared (FT-NIR) spectroscopy	459:493	The data demonstrate that faster cooling rates (>100°C/min) result in trehalose crystallization and protein aggregation as determined by Fourier Transform Near-Infrared (FT-NIR) spectroscopy and size-exclusion chromatography, respectively.
26398200	5	53	theme	frozen	1159:1164	arg1	solutions					1166:1174	frozen solutions	1159:1174	frozen solutions	1159:1174	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	4	54	theme	aggregation	894:904	arg1	amount					873:878	a relatively higher amount	853:878	a relatively higher amount of cumulative aggregation	853:904	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	54	theme	aggregation	894:904	arg1	aggregation					894:904	cumulative aggregation	883:904	cumulative aggregation	883:904	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	2	55	theme	faster	330:335	arg1	rates					345:349	faster cooling rates	330:349	faster cooling rates (>100°C/min)	330:362	The data demonstrate that faster cooling rates (>100°C/min) result in trehalose crystallization and protein aggregation as determined by Fourier Transform Near-Infrared (FT-NIR) spectroscopy and size-exclusion chromatography, respectively.
26398200	2	55	theme	faster	330:335	arg1	>100°C/min					352:361	>100°C/min	352:361	>100°C/min	352:361	The data demonstrate that faster cooling rates (>100°C/min) result in trehalose crystallization and protein aggregation as determined by Fourier Transform Near-Infrared (FT-NIR) spectroscopy and size-exclusion chromatography, respectively.
26398200	4	56	theme	cumulative	883:892	arg1	aggregation					894:904	cumulative aggregation	883:904	cumulative aggregation	883:904	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	57	theme	storage	822:828	arg1	temperatures					830:841	higher (-8°C) and lower (-20°C) storage temperatures	790:841	higher (-8°C) and lower (-20°C) storage temperatures	790:841	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	6	58	theme	0.2-2.4	1286:1292	arg1	ratio					1279:1283	trehalose-mAb (w/w) ratio	1259:1283	trehalose-mAb (w/w) ratio	1259:1283	The results identify an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations.
26398200	5	59	theme	effects	1034:1040	arg1	evaluation					1016:1025	Further evaluation	1008:1025	Further evaluation of the effects of formulation composition	1008:1067	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	5	60	theme	Further	1008:1014	arg1	evaluation					1016:1025	Further evaluation	1008:1025	Further evaluation of the effects of formulation composition	1008:1067	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	3	61	theme	protein	660:666	arg1	stability					668:676	protein stability	660:676	protein stability	660:676	Conversely, at slower cooling rates (≤1°C/min), trehalose remains predominantly amorphous and there is no effect on protein stability.
26398200	6	62	theme	cooled	1392:1397	arg1	formulations					1412:1423	fast cooled (>100°C/min) formulations	1387:1423	fast cooled (>100°C/min) formulations	1387:1423	The results identify an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations.
26398200	6	63	theme	long-term	1353:1361	arg1	storage-even					1370:1381	long-term frozen storage-even	1353:1381	long-term frozen storage-even for fast cooled (>100°C/min) formulations	1353:1423	The results identify an optimal range of trehalose-mAb (w/w) ratio, 0.2-2.4, capable of physically stabilizing mAb formulations during long-term frozen storage-even for fast cooled (>100°C/min) formulations.
26398200	0	64	theme	Composition	65:75	arg1	Effects					54:60	Effects	54:60	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.	0:115	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.
26398200	5	65	theme	formulation	1045:1055	arg1	composition					1057:1067	formulation composition	1045:1067	formulation composition	1045:1067	Further evaluation of the effects of formulation composition suggests that the phase distribution of amorphous and crystallized trehalose dihydrate in frozen solutions depends on the ratio of trehalose to mAb.
26398200	0	66	dep	Aggregation	8:18	arg1	Effects					54:60	Effects	54:60	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.	0:115	Protein Aggregation in Frozen Trehalose Formulations: Effects of Composition, Cooling Rate, and Storage Temperature.
26398200	4	67	theme	storage	693:699	arg1	temperatures					701:712	storage temperatures	693:712	storage temperatures	693:712	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	68	dep	lower	808:812	arg1	-20°C					815:819	-20°C	815:819	-20°C	815:819	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	1	69	theme	formulation	205:215	arg1	composition					217:227	formulation composition	205:227	formulation composition	205:227	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26398200	4	70	theme	higher	962:967	arg1	-8°C					1001:1004	-8°C	1001:1004	-8°C	1001:1004	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	70	theme	higher	962:967	arg1	-14°C					991:995	-14°C	991:995	-14°C	991:995	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	70	theme	higher	962:967	arg1	temperatures					977:988	higher storage temperatures	962:988	higher storage temperatures (-14°C and -8°C)	962:1005	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	4	71	theme	lower	808:812	arg1	temperatures					830:841	higher (-8°C) and lower (-20°C) storage temperatures	790:841	higher (-8°C) and lower (-20°C) storage temperatures	790:841	Evaluation of storage temperatures demonstrates that aggregation increases more rapidly at -14°C compared with higher (-8°C) and lower (-20°C) storage temperatures; however, a relatively higher amount of cumulative aggregation was observed at lower (-20°C) temperature compared with higher storage temperatures (-14°C and -8°C).
26398200	1	72	theme	composition	217:227	arg1	effects					155:161	the effects	151:161	the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions	151:301	This study was designed to assess the effects of cooling rate, storage temperature, and formulation composition on trehalose phase distribution and protein stability in frozen solutions.
26608007	7	0	contain	containing	947:956	arg1	beads					941:945	Various nHAp-alginate-PVP beads	915:945	Various nHAp-alginate-PVP beads containing DS	915:959	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	7	0	contain	containing	947:956	arg2	DS					958:959	DS	958:959	DS	958:959	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	1	1	theme	nano-hydroxyapatite	217:235	arg1	powders					244:250	nano-hydroxyapatite [nHAp] powders	217:250	nano-hydroxyapatite [nHAp] powders	217:250	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	1	2	theme	polymeric	342:350	arg1	systems					352:358	ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems	259:358	ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems	259:358	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	5	3	theme	drug	671:674	arg1	%					699:699	%	699:699	%	699:699	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	5	3	theme	drug	671:674	arg1	efficiency					687:696	drug entrapment efficiency	671:696	drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72%	671:729	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	1	4	theme	[nHAp	237:241	arg1	powders					244:250	nano-hydroxyapatite [nHAp] powders	217:250	nano-hydroxyapatite [nHAp] powders	217:250	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	7	5	theme	release	1053:1059	arg1	model					1039:1043	the Koresmeyer-Peppas model	1017:1043	the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978)	1017:1078	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	5	6	theme	%	729:729	arg1	sizes					748:752	average bead sizes	735:752	average bead sizes of 0.98±0.07 to 1.23±0.15 mm	735:781	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	5	6	theme	%	729:729	arg1	%					699:699	%	699:699	%	699:699	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	5	6	theme	%	729:729	arg1	efficiency					687:696	drug entrapment efficiency	671:696	drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72%	671:729	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	5	7	theme	entrapment	676:685	arg1	%					699:699	%	699:699	%	699:699	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	5	7	theme	entrapment	676:685	arg1	efficiency					687:696	drug entrapment efficiency	671:696	drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72%	671:729	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	2	8	theme	precipitation	394:406	arg1	technique					408:416	precipitation technique	394:416	precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials	394:500	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	4	9	contain	containing	634:643	arg2	DS					645:646	DS	645:646	DS	645:646	nHAp powders was found as 19.04 nm and used to prepare nHAp-alginate-PVP beads containing DS.
26608007	4	9	contain	containing	634:643	arg1	beads					628:632	nHAp-alginate-PVP beads	610:632	nHAp-alginate-PVP beads containing DS	610:646	nHAp powders was found as 19.04 nm and used to prepare nHAp-alginate-PVP beads containing DS.
26608007	4	10	theme	nHAp	555:558	arg1	powders					560:566	nHAp powders	555:566	nHAp powders	555:566	nHAp powders was found as 19.04 nm and used to prepare nHAp-alginate-PVP beads containing DS.
26608007	4	10	theme	nHAp	555:558	arg1	nm					587:588	19.04 nm	581:588	19.04 nm	581:588	nHAp powders was found as 19.04 nm and used to prepare nHAp-alginate-PVP beads containing DS.
26608007	7	11	theme	anomalous	1106:1114	arg1	mechanism					1127:1135	non-Fickian release (anomalous transport) mechanism (n=0.73-0.84)	1085:1149	non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h	1085:1175	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	6	12	theme	Fourier	857:863	arg1	spectroscopy					892:903	Fourier transform-infra red (FTIR) spectroscopy	857:903	Fourier transform-infra red (FTIR) spectroscopy	857:903	These beads were characterized by scanning electron microscopy (SEM) and Fourier transform-infra red (FTIR) spectroscopy analyses.
26608007	1	13	theme	Alginate-based	89:102	arg1	matrices					141:148	Alginate-based bipolymeric-nanobioceramic composite matrices	89:148	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release	89:175	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	7	14	theme	Various	915:921	arg1	beads					941:945	Various nHAp-alginate-PVP beads	915:945	Various nHAp-alginate-PVP beads containing DS	915:959	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	1	15	theme	bipolymeric-nanobioceramic	104:129	arg1	matrices					141:148	Alginate-based bipolymeric-nanobioceramic composite matrices	89:148	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release	89:175	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	6	16	dep	microscopy	836:845	arg1	analyses					905:912	analyses	905:912	analyses	905:912	These beads were characterized by scanning electron microscopy (SEM) and Fourier transform-infra red (FTIR) spectroscopy analyses.
26608007	3	17	theme	particle	515:522	arg1	size					524:527	The average particle size	503:527	The average particle size of these	503:536	The average particle size of these was synthesized.
26608007	3	18	theme	average	507:513	arg1	size					524:527	The average particle size	503:527	The average particle size of these	503:536	The average particle size of these was synthesized.
26608007	1	19	theme	composite	131:139	arg1	matrices					141:148	Alginate-based bipolymeric-nanobioceramic composite matrices	89:148	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release	89:175	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	1	20	theme	powders	244:250	arg1	incorporation					200:212	incorporation	200:212	incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems	200:358	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	0	21	theme	bipolymeric-nanobioceramic	15:40	arg1	matrices					52:59	bipolymeric-nanobioceramic composite matrices	15:59	bipolymeric-nanobioceramic composite matrices	15:59	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release.
26608007	7	22	theme	Koresmeyer-Peppas	1021:1037	arg1	model					1039:1043	the Koresmeyer-Peppas model	1017:1043	the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978)	1017:1078	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	2	23	theme	calcium	424:430	arg1	[H3PO4					477:482	orthophosphoric acid [H3PO4]	456:483	orthophosphoric acid [H3PO4]	456:483	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	23	theme	calcium	424:430	arg1	OH					446:447	OH	446:447	OH	446:447	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	23	theme	calcium	424:430	arg1	materials					492:500	raw materials	488:500	raw materials	488:500	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	23	theme	calcium	424:430	arg1	[Ca					442:444	calcium hydroxide [Ca(OH)2]	424:450	calcium hydroxide [Ca(OH)2]	424:450	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	24	theme	acid	472:475	arg1	[H3PO4					477:482	orthophosphoric acid [H3PO4]	456:483	orthophosphoric acid [H3PO4]	456:483	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	24	theme	acid	472:475	arg1	materials					492:500	raw materials	488:500	raw materials	488:500	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	24	theme	acid	472:475	arg1	[Ca					442:444	calcium hydroxide [Ca(OH)2]	424:450	calcium hydroxide [Ca(OH)2]	424:450	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	4	25	used	used	594:597	arg2	nm					587:588	19.04 nm	581:588	19.04 nm	581:588	nHAp powders was found as 19.04 nm and used to prepare nHAp-alginate-PVP beads containing DS.
26608007	4	25	used	used	594:597	arg2	powders					560:566	nHAp powders	555:566	nHAp powders	555:566	nHAp powders was found as 19.04 nm and used to prepare nHAp-alginate-PVP beads containing DS.
26608007	1	26	theme	ionotropically-gelled	259:279	arg1	alginate-poly					301:313	ionotropically-gelled calcium ion-induced alginate-poly	259:313	ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems	259:358	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	1	26	theme	ionotropically-gelled	259:279	arg1	pyrrolidone					322:332	vinyl pyrrolidone	316:332	vinyl pyrrolidone	316:332	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	4	27	theme	nHAp-alginate-PVP	610:626	arg1	beads					628:632	nHAp-alginate-PVP beads	610:632	nHAp-alginate-PVP beads containing DS	610:646	nHAp powders was found as 19.04 nm and used to prepare nHAp-alginate-PVP beads containing DS.
26608007	1	28	theme	sustained	154:162	arg1	release					169:175	sustained drug release	154:175	sustained drug release	154:175	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	6	29	theme	red	881:883	arg1	spectroscopy					892:903	Fourier transform-infra red (FTIR) spectroscopy	857:903	Fourier transform-infra red (FTIR) spectroscopy	857:903	These beads were characterized by scanning electron microscopy (SEM) and Fourier transform-infra red (FTIR) spectroscopy analyses.
26608007	1	30	theme	calcium	281:287	arg1	alginate-poly					301:313	ionotropically-gelled calcium ion-induced alginate-poly	259:313	ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems	259:358	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	1	30	theme	calcium	281:287	arg1	pyrrolidone					322:332	vinyl pyrrolidone	316:332	vinyl pyrrolidone	316:332	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	0	31	theme	composite	42:50	arg1	matrices					52:59	bipolymeric-nanobioceramic composite matrices	15:59	bipolymeric-nanobioceramic composite matrices	15:59	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release.
26608007	7	32	theme	drug	1155:1158	arg1	release					1160:1166	drug release	1155:1166	drug release over 8 h	1155:1175	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	1	33	theme	drug	164:167	arg1	release					169:175	sustained drug release	154:175	sustained drug release	154:175	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	1	34	theme	ion-induced	289:299	arg1	alginate-poly					301:313	ionotropically-gelled calcium ion-induced alginate-poly	259:313	ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems	259:358	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	1	34	theme	ion-induced	289:299	arg1	pyrrolidone					322:332	vinyl pyrrolidone	316:332	vinyl pyrrolidone	316:332	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	0	35	theme	sustained	65:73	arg1	release					80:86	sustained drug release	65:86	sustained drug release	65:86	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release.
26608007	7	36	theme	nHAp-alginate-PVP	923:939	arg1	beads					941:945	Various nHAp-alginate-PVP beads	915:945	Various nHAp-alginate-PVP beads containing DS	915:959	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	2	37	theme	raw	488:490	arg1	[H3PO4					477:482	orthophosphoric acid [H3PO4]	456:483	orthophosphoric acid [H3PO4]	456:483	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	37	theme	raw	488:490	arg1	materials					492:500	raw materials	488:500	raw materials	488:500	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	37	theme	raw	488:490	arg1	[Ca					442:444	calcium hydroxide [Ca(OH)2]	424:450	calcium hydroxide [Ca(OH)2]	424:450	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	38	theme	orthophosphoric	456:470	arg1	[H3PO4					477:482	orthophosphoric acid [H3PO4]	456:483	orthophosphoric acid [H3PO4]	456:483	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	38	theme	orthophosphoric	456:470	arg1	materials					492:500	raw materials	488:500	raw materials	488:500	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	38	theme	orthophosphoric	456:470	arg1	[Ca					442:444	calcium hydroxide [Ca(OH)2]	424:450	calcium hydroxide [Ca(OH)2]	424:450	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	1	39	theme	alginate-poly	301:313	arg1	systems					352:358	ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems	259:358	ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems	259:358	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	5	40	theme	mm	780:781	arg1	sizes					748:752	average bead sizes	735:752	average bead sizes of 0.98±0.07 to 1.23±0.15 mm	735:781	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	5	40	theme	mm	780:781	arg1	%					699:699	%	699:699	%	699:699	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	5	40	theme	mm	780:781	arg1	efficiency					687:696	drug entrapment efficiency	671:696	drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72%	671:729	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	6	41	theme	electron	827:834	arg1	SEM					848:850	SEM	848:850	SEM	848:850	These beads were characterized by scanning electron microscopy (SEM) and Fourier transform-infra red (FTIR) spectroscopy analyses.
26608007	6	41	theme	electron	827:834	arg1	microscopy					836:845	scanning electron microscopy	818:845	scanning electron microscopy (SEM)	818:851	These beads were characterized by scanning electron microscopy (SEM) and Fourier transform-infra red (FTIR) spectroscopy analyses.
26608007	3	42	theme	these	532:536	arg1	size					524:527	The average particle size	503:527	The average particle size of these	503:536	The average particle size of these was synthesized.
26608007	5	43	theme	average	735:741	arg1	sizes					748:752	average bead sizes	735:752	average bead sizes of 0.98±0.07 to 1.23±0.15 mm	735:781	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	6	44	theme	scanning	818:825	arg1	SEM					848:850	SEM	848:850	SEM	848:850	These beads were characterized by scanning electron microscopy (SEM) and Fourier transform-infra red (FTIR) spectroscopy analyses.
26608007	6	44	theme	scanning	818:825	arg1	microscopy					836:845	scanning electron microscopy	818:845	scanning electron microscopy (SEM)	818:851	These beads were characterized by scanning electron microscopy (SEM) and Fourier transform-infra red (FTIR) spectroscopy analyses.
26608007	0	45	theme	drug	75:78	arg1	release					80:86	sustained drug release	65:86	sustained drug release	65:86	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release.
26608007	5	46	theme	bead	743:746	arg1	sizes					748:752	average bead sizes	735:752	average bead sizes of 0.98±0.07 to 1.23±0.15 mm	735:781	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	7	47	theme	non-Fickian	1085:1095	arg1	mechanism					1127:1135	non-Fickian release (anomalous transport) mechanism (n=0.73-0.84)	1085:1149	non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h	1085:1175	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	7	48	theme	drug	991:994	arg1	release					996:1002	prolonged sustained drug release	971:1002	prolonged sustained drug release	971:1002	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	7	49	theme	transport	1116:1124	arg1	mechanism					1127:1135	non-Fickian release (anomalous transport) mechanism (n=0.73-0.84)	1085:1149	non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h	1085:1175	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	2	50	theme	nHAp	361:364	arg1	powders					366:372	nHAp powders	361:372	nHAp powders	361:372	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	6	51	theme	transform-infra	865:879	arg1	spectroscopy					892:903	Fourier transform-infra red (FTIR) spectroscopy	857:903	Fourier transform-infra red (FTIR) spectroscopy	857:903	These beads were characterized by scanning electron microscopy (SEM) and Fourier transform-infra red (FTIR) spectroscopy analyses.
26608007	1	52	theme	vinyl	316:320	arg1	alginate-poly					301:313	ionotropically-gelled calcium ion-induced alginate-poly	259:313	ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems	259:358	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	1	52	theme	vinyl	316:320	arg1	pyrrolidone					322:332	vinyl pyrrolidone	316:332	vinyl pyrrolidone	316:332	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26608007	7	53	theme	release	1097:1103	arg1	mechanism					1127:1135	non-Fickian release (anomalous transport) mechanism (n=0.73-0.84)	1085:1149	non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h	1085:1175	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	6	54	theme	FTIR	886:889	arg1	spectroscopy					892:903	Fourier transform-infra red (FTIR) spectroscopy	857:903	Fourier transform-infra red (FTIR) spectroscopy	857:903	These beads were characterized by scanning electron microscopy (SEM) and Fourier transform-infra red (FTIR) spectroscopy analyses.
26608007	2	55	theme	hydroxide	432:440	arg1	[H3PO4					477:482	orthophosphoric acid [H3PO4]	456:483	orthophosphoric acid [H3PO4]	456:483	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	55	theme	hydroxide	432:440	arg1	OH					446:447	OH	446:447	OH	446:447	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	55	theme	hydroxide	432:440	arg1	materials					492:500	raw materials	488:500	raw materials	488:500	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	2	55	theme	hydroxide	432:440	arg1	[Ca					442:444	calcium hydroxide [Ca(OH)2]	424:450	calcium hydroxide [Ca(OH)2]	424:450	nHAp powders were synthesized by precipitation technique using calcium hydroxide [Ca(OH)2] and orthophosphoric acid [H3PO4] as raw materials.
26608007	7	56	theme	prolonged	971:979	arg1	release					996:1002	prolonged sustained drug release	971:1002	prolonged sustained drug release	971:1002	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	5	57	dep	1.23±0.15	770:778	arg1	to					767:768	to	767:768	to	767:768	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	7	58	theme	drug	1048:1051	arg1	R2=0.9908-0.9978					1062:1077	R2=0.9908-0.9978	1062:1077	R2=0.9908-0.9978	1062:1077	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	7	58	theme	drug	1048:1051	arg1	release					1053:1059	drug release	1048:1059	drug release (R2=0.9908-0.9978)	1048:1078	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	7	59	theme	sustained	981:989	arg1	release					996:1002	prolonged sustained drug release	971:1002	prolonged sustained drug release	971:1002	Various nHAp-alginate-PVP beads containing DS exhibited prolonged sustained drug release and followed the Koresmeyer-Peppas model of drug release (R2=0.9908-0.9978) with non-Fickian release (anomalous transport) mechanism (n=0.73-0.84) for drug release over 8 h.
26608007	5	60	dep	94.45±3.72	719:728	arg1	to					716:717	to	716:717	to	716:717	These beads exhibited drug entrapment efficiency (%) of 65.82±1.88 to 94.45±3.72% and average bead sizes of 0.98±0.07 to 1.23±0.15 mm.
26608007	1	61	theme	blends	335:340	arg1	systems					352:358	ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems	259:358	ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems	259:358	Alginate-based bipolymeric-nanobioceramic composite matrices for sustained drug release were developed through incorporation of nano-hydroxyapatite [nHAp] powders within ionotropically-gelled calcium ion-induced alginate-poly (vinyl pyrrolidone) blends polymeric systems.
26347257	0	0	theme	molecular	87:95	arg1	structure					103:111	the mucus molecular sieve structure	77:111	the mucus molecular sieve structure	77:111	Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure.
26347257	9	1	theme	molecular	1293:1301	arg1	effect					1309:1314	the molecular sieve effect	1289:1314	the molecular sieve effect	1289:1314	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	8	2	theme	simulated	1013:1021	arg1	system					1037:1042	simulated vaginal mucus system	1013:1042	simulated vaginal mucus system	1013:1042	They exhibited different potential of opening mesh size of molecular sieve in simulated vaginal mucus system.
26347257	0	3	theme	mucus	81:85	arg1	structure					103:111	the mucus molecular sieve structure	77:111	the mucus molecular sieve structure	77:111	Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure.
26347257	4	4	theme	Pluronic	551:558	arg1	F127					566:569	F127	566:569	F127	566:569	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	4	4	theme	Pluronic	551:558	arg1	F127					560:563	Pluronic F127	551:563	Pluronic F127 (F127)	551:570	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	10	5	theme	powder	1457:1462	arg1	formulations					1464:1475	powder formulations	1457:1475	powder formulations with optimized compositions	1457:1503	This study suggests that powder formulations with optimized compositions have the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery.
26347257	9	6	with	formulation	1054:1064	arg1	HPMC					1090:1093	0.1% HPMC	1085:1093	0.1% HPMC	1085:1093	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	9	6	with	formulation	1054:1064	arg1	F127					1076:1079	0.6% F127	1071:1079	0.6% F127	1071:1079	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	5	7	theme	μm	756:757	arg1	diameters					738:746	diameters	738:746	diameters of 5-15 μm	738:757	Highly dispersed dry powders with diameters of 5-15 μm were produced.
26347257	9	8	theme	effect	1309:1314	arg1	modulation					1275:1284	the resulting modulation	1261:1284	the resulting modulation of the molecular sieve effect	1261:1314	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	8	9	theme	mucus	1031:1035	arg1	system					1037:1042	simulated vaginal mucus system	1013:1042	simulated vaginal mucus system	1013:1042	They exhibited different potential of opening mesh size of molecular sieve in simulated vaginal mucus system.
26347257	4	10	theme	spray-drying	680:691	arg1	technique					693:701	an ultrasound-assisted spray-drying technique	657:701	an ultrasound-assisted spray-drying technique	657:701	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	0	11	theme	sieve	97:101	arg1	structure					103:111	the mucus molecular sieve structure	77:111	the mucus molecular sieve structure	77:111	Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure.
26347257	3	12	contain	containing	499:508	arg1	powders					491:497	spray-dried powders	479:497	spray-dried powders containing siRNA-loaded nanoparticles	479:535	In order to overcome this barrier and enhance vaginal mucus penetration, we prepared spray-dried powders containing siRNA-loaded nanoparticles.
26347257	3	12	contain	containing	499:508	arg2	nanoparticles					523:535	siRNA-loaded nanoparticles	510:535	siRNA-loaded nanoparticles	510:535	In order to overcome this barrier and enhance vaginal mucus penetration, we prepared spray-dried powders containing siRNA-loaded nanoparticles.
26347257	9	13	theme	gel	1155:1157	arg1	size					1137:1140	the pore size	1128:1140	the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel	1128:1258	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	9	14	theme	maximum	1108:1114	arg1	increase					1116:1123	the maximum increase	1104:1123	the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel	1104:1258	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	3	15	theme	siRNA-loaded	510:521	arg1	nanoparticles					523:535	siRNA-loaded nanoparticles	510:535	siRNA-loaded nanoparticles	510:535	In order to overcome this barrier and enhance vaginal mucus penetration, we prepared spray-dried powders containing siRNA-loaded nanoparticles.
26347257	2	16	theme	mucus	279:283	arg1	layer					285:289	the densely cross-linked mucus layer	254:289	the densely cross-linked mucus layer on the vaginal wall	254:309	However, the densely cross-linked mucus layer on the vaginal wall severely restricts nanoparticle-mediated siRNA delivery to the vaginal epithelium.
26347257	3	17	theme	mucus	448:452	arg1	penetration					454:464	vaginal mucus penetration	440:464	vaginal mucus penetration	440:464	In order to overcome this barrier and enhance vaginal mucus penetration, we prepared spray-dried powders containing siRNA-loaded nanoparticles.
26347257	9	18	theme	17.8-fold	1327:1335	arg1	improvement					1337:1347	a 17.8-fold improvement	1325:1347	a 17.8-fold improvement of siRNA delivery in vaginal tract	1325:1382	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	6	19	theme	sustained	826:834	arg1	release					836:842	sustained release	826:842	sustained release	826:842	These powders showed effective siRNA protection and sustained release.
26347257	2	20	theme	cross-linked	266:277	arg1	layer					285:289	the densely cross-linked mucus layer	254:289	the densely cross-linked mucus layer on the vaginal wall	254:309	However, the densely cross-linked mucus layer on the vaginal wall severely restricts nanoparticle-mediated siRNA delivery to the vaginal epithelium.
26347257	10	21	with	formulations	1464:1475	arg1	compositions					1492:1503	optimized compositions	1482:1503	optimized compositions	1482:1503	This study suggests that powder formulations with optimized compositions have the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery.
26347257	1	22	theme	promising	172:180	arg1	strategy					182:189	a promising strategy	170:189	a promising strategy for the prevention and treatment of vaginal diseases	170:242	Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases.
26347257	4	23	theme	methyl	587:592	arg1	HPMC					605:608	HPMC	605:608	HPMC	605:608	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	4	23	theme	methyl	587:592	arg1	cellulose					594:602	hydroxypropyl methyl cellulose	573:602	hydroxypropyl methyl cellulose (HPMC)	573:609	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	10	24	theme	optimized	1482:1490	arg1	compositions					1492:1503	optimized compositions	1482:1503	optimized compositions	1482:1503	This study suggests that powder formulations with optimized compositions have the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery.
26347257	4	25	theme	ultrasound-assisted	660:678	arg1	technique					693:701	an ultrasound-assisted spray-drying technique	657:701	an ultrasound-assisted spray-drying technique	657:701	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	0	26	theme	Spray-dried	0:10	arg1	powders					12:18	Spray-dried powders	0:18	Spray-dried powders	0:18	Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure.
26347257	4	27	theme	hydroxypropyl	573:585	arg1	HPMC					605:608	HPMC	605:608	HPMC	605:608	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	4	27	theme	hydroxypropyl	573:585	arg1	cellulose					594:602	hydroxypropyl methyl cellulose	573:602	hydroxypropyl methyl cellulose (HPMC)	573:609	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	9	28	theme	pore	1132:1135	arg1	size					1137:1140	the pore size	1128:1140	the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel	1128:1258	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	3	29	theme	vaginal	440:446	arg1	penetration					454:464	vaginal mucus penetration	440:464	vaginal mucus penetration	440:464	In order to overcome this barrier and enhance vaginal mucus penetration, we prepared spray-dried powders containing siRNA-loaded nanoparticles.
26347257	0	30	theme	vaginal	28:34	arg1	delivery					42:49	vaginal siRNA delivery	28:49	vaginal siRNA delivery	28:49	Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure.
26347257	2	31	link	cross-linked	266:277	arg1	layer					285:289	the densely cross-linked mucus layer	254:289	the densely cross-linked mucus layer on the vaginal wall	254:309	However, the densely cross-linked mucus layer on the vaginal wall severely restricts nanoparticle-mediated siRNA delivery to the vaginal epithelium.
26347257	6	32	theme	siRNA	805:809	arg1	protection					811:820	effective siRNA protection	795:820	effective siRNA protection	795:820	These powders showed effective siRNA protection and sustained release.
26347257	2	33	theme	vaginal	298:304	arg1	wall					306:309	the vaginal wall	294:309	the vaginal wall	294:309	However, the densely cross-linked mucus layer on the vaginal wall severely restricts nanoparticle-mediated siRNA delivery to the vaginal epithelium.
26347257	7	34	theme	powders	885:891	arg1	properties					867:876	The mucus-penetrating properties	845:876	The mucus-penetrating properties of the powders	845:891	The mucus-penetrating properties of the powders differed depending on their compositions.
26347257	9	35	theme	vaginal	1176:1182	arg1	mucus					1184:1188	vaginal mucus	1176:1188	vaginal mucus	1176:1188	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	9	36	theme	delivery	1358:1365	arg1	delivery					1404:1411	effective siRNA delivery	1388:1411	effective siRNA delivery to the epithelium	1388:1429	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	9	36	theme	delivery	1358:1365	arg1	improvement					1337:1347	a 17.8-fold improvement	1325:1347	a 17.8-fold improvement of siRNA delivery in vaginal tract	1325:1382	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	9	37	from	improvement	1337:1347	arg1	tract					1378:1382	vaginal tract	1370:1382	vaginal tract	1370:1382	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	1	38	theme	Vaginal	114:120	arg1	delivery					152:159	Vaginal small interfering RNA (siRNA) delivery	114:159	Vaginal small interfering RNA (siRNA) delivery	114:159	Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases.
26347257	8	39	theme	opening	973:979	arg1	size					986:989	opening mesh size	973:989	opening mesh size of molecular sieve	973:1008	They exhibited different potential of opening mesh size of molecular sieve in simulated vaginal mucus system.
26347257	3	40	theme	spray-dried	479:489	arg1	powders					491:497	spray-dried powders	479:497	spray-dried powders containing siRNA-loaded nanoparticles	479:535	In order to overcome this barrier and enhance vaginal mucus penetration, we prepared spray-dried powders containing siRNA-loaded nanoparticles.
26347257	9	41	theme	powder	1047:1052	arg1	formulation					1054:1064	A powder formulation	1045:1064	A powder formulation with 0.6% F127 and 0.1% HPMC	1045:1093	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	2	42	theme	siRNA	352:356	arg1	delivery					358:365	nanoparticle-mediated siRNA delivery	330:365	nanoparticle-mediated siRNA delivery	330:365	However, the densely cross-linked mucus layer on the vaginal wall severely restricts nanoparticle-mediated siRNA delivery to the vaginal epithelium.
26347257	8	43	theme	size	986:989	arg1	potential					960:968	different potential	950:968	different potential of opening mesh size of molecular sieve	950:1008	They exhibited different potential of opening mesh size of molecular sieve in simulated vaginal mucus system.
26347257	10	44	theme	siRNA	1606:1610	arg1	delivery					1612:1619	effective vaginal siRNA delivery	1588:1619	effective vaginal siRNA delivery	1588:1619	This study suggests that powder formulations with optimized compositions have the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery.
26347257	9	45	theme	0.1	1085:1087	arg1	%					1088:1088	%	1088:1088	%	1088:1088	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	0	46	theme	siRNA	36:40	arg1	delivery					42:49	vaginal siRNA delivery	28:49	vaginal siRNA delivery	28:49	Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure.
26347257	9	47	theme	vaginal	1370:1376	arg1	tract					1378:1382	vaginal tract	1370:1382	vaginal tract	1370:1382	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	9	48	theme	model	1149:1153	arg1	gel					1155:1157	the model gel	1145:1157	the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel	1145:1258	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	9	49	theme	siRNA	1352:1356	arg1	delivery					1358:1365	siRNA delivery	1352:1365	siRNA delivery	1352:1365	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	6	50	theme	effective	795:803	arg1	protection					811:820	effective siRNA protection	795:820	effective siRNA protection	795:820	These powders showed effective siRNA protection and sustained release.
26347257	1	51	theme	vaginal	227:233	arg1	diseases					235:242	vaginal diseases	227:242	vaginal diseases	227:242	Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases.
26347257	10	52	theme	steric	1537:1542	arg1	barrier					1544:1550	the steric barrier	1533:1550	the steric barrier posed by mucus	1533:1565	This study suggests that powder formulations with optimized compositions have the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery.
26347257	10	53	theme	vaginal	1598:1604	arg1	delivery					1612:1619	effective vaginal siRNA delivery	1588:1619	effective vaginal siRNA delivery	1588:1619	This study suggests that powder formulations with optimized compositions have the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery.
26347257	7	54	theme	mucus-penetrating	849:865	arg1	properties					867:876	The mucus-penetrating properties	845:876	The mucus-penetrating properties of the powders	845:891	The mucus-penetrating properties of the powders differed depending on their compositions.
26347257	8	55	theme	mesh	981:984	arg1	size					986:989	opening mesh size	973:989	opening mesh size of molecular sieve	973:1008	They exhibited different potential of opening mesh size of molecular sieve in simulated vaginal mucus system.
26347257	1	56	theme	small	122:126	arg1	RNA					140:142	small interfering RNA	122:142	Vaginal small interfering RNA (siRNA) delivery	114:159	Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases.
26347257	1	56	theme	small	122:126	arg1	siRNA					145:149	siRNA	145:149	siRNA	145:149	Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases.
26347257	9	57	theme	0.6	1071:1073	arg1	%					1074:1074	%	1074:1074	%	1074:1074	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	5	58	theme	dispersed	711:719	arg1	powders					725:731	Highly dispersed dry powders	704:731	Highly dispersed dry powders with diameters of 5-15 μm	704:757	Highly dispersed dry powders with diameters of 5-15 μm were produced.
26347257	8	59	theme	molecular	994:1002	arg1	sieve					1004:1008	molecular sieve	994:1008	molecular sieve	994:1008	They exhibited different potential of opening mesh size of molecular sieve in simulated vaginal mucus system.
26347257	1	60	theme	interfering	128:138	arg1	RNA					140:142	small interfering RNA	122:142	Vaginal small interfering RNA (siRNA) delivery	114:159	Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases.
26347257	1	60	theme	interfering	128:138	arg1	siRNA					145:149	siRNA	145:149	siRNA	145:149	Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases.
26347257	9	61	theme	%	1074:1074	arg1	F127					1076:1079	0.6% F127	1071:1079	0.6% F127	1071:1079	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	9	62	theme	effective	1388:1396	arg1	delivery					1404:1411	effective siRNA delivery	1388:1411	effective siRNA delivery to the epithelium	1388:1429	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	5	63	theme	dry	721:723	arg1	powders					725:731	Highly dispersed dry powders	704:731	Highly dispersed dry powders with diameters of 5-15 μm	704:757	Highly dispersed dry powders with diameters of 5-15 μm were produced.
26347257	9	64	theme	resulting	1265:1273	arg1	modulation					1275:1284	the resulting modulation	1261:1284	the resulting modulation of the molecular sieve effect	1261:1314	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	1	65	theme	RNA	140:142	arg1	delivery					152:159	Vaginal small interfering RNA (siRNA) delivery	114:159	Vaginal small interfering RNA (siRNA) delivery	114:159	Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases.
26347257	9	66	theme	siRNA	1398:1402	arg1	delivery					1404:1411	effective siRNA delivery	1388:1411	effective siRNA delivery to the epithelium	1388:1429	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	9	67	from	delivery	1404:1411	arg1	tract					1378:1382	vaginal tract	1370:1382	vaginal tract	1370:1382	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	4	68	with	Powders	538:544	arg1	F127					566:569	F127	566:569	F127	566:569	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	4	68	with	Powders	538:544	arg1	mannitol					616:623	mannitol	616:623	mannitol	616:623	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	4	68	with	Powders	538:544	arg1	F127					560:563	Pluronic F127	551:563	Pluronic F127 (F127)	551:570	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	4	68	with	Powders	538:544	arg1	HPMC					605:608	HPMC	605:608	HPMC	605:608	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	4	68	with	Powders	538:544	arg1	cellulose					594:602	hydroxypropyl methyl cellulose	573:602	hydroxypropyl methyl cellulose (HPMC)	573:609	Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique.
26347257	8	69	theme	different	950:958	arg1	potential					960:968	different potential	950:968	different potential of opening mesh size of molecular sieve	950:1008	They exhibited different potential of opening mesh size of molecular sieve in simulated vaginal mucus system.
26347257	2	70	theme	nanoparticle-mediated	330:350	arg1	delivery					358:365	nanoparticle-mediated siRNA delivery	330:365	nanoparticle-mediated siRNA delivery	330:365	However, the densely cross-linked mucus layer on the vaginal wall severely restricts nanoparticle-mediated siRNA delivery to the vaginal epithelium.
26347257	10	71	contain	have	1505:1508	arg1	formulations					1464:1475	powder formulations	1457:1475	powder formulations with optimized compositions	1457:1503	This study suggests that powder formulations with optimized compositions have the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery.
26347257	10	71	contain	have	1505:1508	arg2	potential					1514:1522	the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery	1510:1619	the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery	1510:1619	This study suggests that powder formulations with optimized compositions have the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery.
26347257	8	72	theme	sieve	1004:1008	arg1	size					986:989	opening mesh size	973:989	opening mesh size of molecular sieve	973:1008	They exhibited different potential of opening mesh size of molecular sieve in simulated vaginal mucus system.
26347257	9	73	from	increase	1116:1123	arg1	size					1137:1140	the pore size	1128:1140	the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel	1128:1258	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	2	74	from	layer	285:289	arg1	wall					306:309	the vaginal wall	294:309	the vaginal wall	294:309	However, the densely cross-linked mucus layer on the vaginal wall severely restricts nanoparticle-mediated siRNA delivery to the vaginal epithelium.
26347257	1	75	theme	diseases	235:242	arg1	prevention					199:208	prevention	199:208	prevention	199:208	Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases.
26347257	1	75	theme	diseases	235:242	arg1	treatment					214:222	treatment	214:222	treatment	214:222	Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases.
26347257	5	76	with	powders	725:731	arg1	diameters					738:746	diameters	738:746	diameters of 5-15 μm	738:757	Highly dispersed dry powders with diameters of 5-15 μm were produced.
26347257	9	77	theme	sieve	1303:1307	arg1	effect					1309:1314	the molecular sieve effect	1289:1314	the molecular sieve effect	1289:1314	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	1	78	dep	prevention	199:208	arg1	the					195:197	the	195:197	the	195:197	Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases.
26347257	8	79	theme	vaginal	1023:1029	arg1	system					1037:1042	simulated vaginal mucus system	1013:1042	simulated vaginal mucus system	1013:1042	They exhibited different potential of opening mesh size of molecular sieve in simulated vaginal mucus system.
26347257	2	80	theme	vaginal	374:380	arg1	epithelium					382:391	the vaginal epithelium	370:391	the vaginal epithelium	370:391	However, the densely cross-linked mucus layer on the vaginal wall severely restricts nanoparticle-mediated siRNA delivery to the vaginal epithelium.
26347257	9	81	theme	%	1088:1088	arg1	HPMC					1090:1093	0.1% HPMC	1085:1093	0.1% HPMC	1085:1093	A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA delivery in vaginal tract and effective siRNA delivery to the epithelium.
26347257	10	82	theme	effective	1588:1596	arg1	delivery					1612:1619	effective vaginal siRNA delivery	1588:1619	effective vaginal siRNA delivery	1588:1619	This study suggests that powder formulations with optimized compositions have the potential to alter the steric barrier posed by mucus and hold promise for effective vaginal siRNA delivery.
28929656	4	0	theme	soluble	950:956	arg1	polysaccharides					958:972	soluble polysaccharides	950:972	soluble polysaccharides	950:972	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	0	1	from	utilization	14:24	arg1	tissues					89:95	different tissues	79:95	different tissues of Abelmoschus manihot]	79:119	[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].
28929656	1	2	from	composition	175:185	arg1	leaf					221:224	leaf	221:224	leaf	221:224	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	2	from	composition	175:185	arg1	stem					215:218	stem	215:218	stem	215:218	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	2	from	composition	175:185	arg1	root					209:212	root	209:212	root	209:212	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	2	from	composition	175:185	arg1	flower					230:235	flower	230:235	flower	230:235	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	2	from	composition	175:185	arg1	tissues					200:206	different tissues	190:206	different tissues (root, stem, leaf and flower)	190:236	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	3	3	from	rich	511:514	arg1	compositions					544:555	the resourceful chemical compositions	519:555	the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3'-O-glucoside, rutin and quercetin	519:701	The flowers are rich in the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3'-O-glucoside, rutin and quercetin.
28929656	2	4	theme	UV-Vis	453:458	arg1	spectrophotpmetry					460:476	UV-Vis spectrophotpmetry	453:476	UV-Vis spectrophotpmetry	453:476	The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS.
28929656	4	5	from	rich	853:856	arg1	celluloses					889:898	celluloses	889:898	celluloses	889:898	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	5	from	rich	853:856	arg1	polysaccharides					869:883	soluble polysaccharides	861:883	soluble polysaccharides	861:883	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	5	6	contain	have	1126:1129	arg1	flowers					1118:1124	the flowers	1114:1124	the flowers	1114:1124	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	5	6	contain	have	1126:1129	arg2	content					1143:1149	the highest content	1131:1149	the highest content of amino acids(4.737 mg•g⁻¹)	1131:1178	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	5	7	dep	the	1187:1189	arg1	leaves					1191:1196	leaves	1191:1196	leaves	1191:1196	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	1	8	theme	resourceful	154:164	arg1	composition					175:185	the resourceful chemical composition	150:185	the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot	150:259	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	0	9	theme	Abelmoschus	100:110	arg1	manihot					112:118	Abelmoschus manihot]	100:119	Abelmoschus manihot]	100:119	[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].
28929656	0	10	from	[Analysis	0:8	arg1	tissues					89:95	different tissues	79:95	different tissues of Abelmoschus manihot]	79:119	[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].
28929656	4	11	theme	soluble	861:867	arg1	polysaccharides					869:883	soluble polysaccharides	861:883	soluble polysaccharides	861:883	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	2	12	from	flavonoids	301:310	arg1	tissues					394:400	the different tissues	380:400	the different tissues of A. manihot	380:414	The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS.
28929656	4	13	theme	tissues.Different	809:825	arg1	rich					853:856	rich	853:856	rich	853:856	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	13	theme	tissues.Different	809:825	arg1	tissues					827:833	the other tissues.Different tissues	799:833	the other tissues.Different tissues of A. manihot	799:847	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	14	theme	highest	923:929	arg1	content					931:937	the highest content	919:937	the highest content(19.76%) of soluble polysaccharides	919:972	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	14	theme	highest	923:929	arg1	%					944:944	19.76%	939:944	19.76%	939:944	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	2	15	from	polysaccharides	321:335	arg1	tissues					394:400	the different tissues	380:400	the different tissues of A. manihot	380:414	The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS.
28929656	1	16	theme	Abelmoschus	241:251	arg1	manihot					253:259	Abelmoschus manihot	241:259	Abelmoschus manihot	241:259	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	5	17	theme	highest	1135:1141	arg1	content					1143:1149	the highest content	1131:1149	the highest content of amino acids(4.737 mg•g⁻¹)	1131:1178	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	5	18	theme	nucleosides	1226:1236	arg1	content					1215:1221	the highest content	1203:1221	the highest content of nucleosides (1.474 mg•g⁻¹)	1203:1251	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	1	19	theme	chemical	166:173	arg1	composition					175:185	the resourceful chemical composition	150:185	the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot	150:259	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	0	20	theme	manihot	112:118	arg1	tissues					89:95	different tissues	79:95	different tissues of Abelmoschus manihot]	79:119	[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].
28929656	2	21	from	cellulose	338:346	arg1	tissues					394:400	the different tissues	380:400	the different tissues of A. manihot	380:414	The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS.
28929656	6	22	theme	scientific	1419:1428	arg1	basis					1430:1434	a scientific basis	1417:1434	a scientific basis for the utilization and industrial development of A. manihot plants	1417:1502	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	6	23	from	rich	1268:1271	arg1	compositions					1301:1312	the resourceful chemical compositions	1276:1312	the resourceful chemical compositions	1276:1312	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	0	24	theme	different	79:87	arg1	tissues					89:95	different tissues	79:95	different tissues of Abelmoschus manihot]	79:119	[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].
28929656	4	25	theme	manihot	841:847	arg1	rich					853:856	rich	853:856	rich	853:856	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	25	theme	manihot	841:847	arg1	tissues					827:833	the other tissues.Different tissues	799:833	the other tissues.Different tissues of A. manihot	799:847	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	5	26	located	detected	1086:1093	arg2	Total					1039:1043	Total	1039:1043	Total of 21 amino acids and 9 nucleosides	1039:1079	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	5	26	located	detected	1086:1093	arg1	plant					1103:1107	this plant	1098:1107	this plant	1098:1107	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	2	27	theme	amino	365:369	arg1	acids					371:375	amino acids	365:375	amino acids in the different tissues of A. manihot	365:414	The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS.
28929656	1	28	theme	manihot	253:259	arg1	composition					175:185	the resourceful chemical composition	150:185	the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot	150:259	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	4	29	theme	A.	838:839	arg1	manihot					841:847	A. manihot	838:847	A. manihot	838:847	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	2	30	from	nucleosides	349:359	arg1	tissues					394:400	the different tissues	380:400	the different tissues of A. manihot	380:414	The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS.
28929656	4	31	theme	flavonoids	731:740	arg1	content					714:720	The total content	704:720	The total content of these flavonoids	704:740	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	31	theme	flavonoids	731:740	arg1	mg•g-1					752:757	25.450 mg•g-1	745:757	25.450 mg•g-1 in the flowers	745:772	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	32	contain	have	914:917	arg1	stems					908:912	the stems	904:912	the stems	904:912	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	32	contain	have	914:917	arg2	%					944:944	19.76%	939:944	19.76%	939:944	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	32	contain	have	914:917	arg2	content					931:937	the highest content	919:937	the highest content(19.76%) of soluble polysaccharides	919:972	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	1	33	theme	different	190:198	arg1	leaf					221:224	leaf	221:224	leaf	221:224	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	33	theme	different	190:198	arg1	stem					215:218	stem	215:218	stem	215:218	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	33	theme	different	190:198	arg1	root					209:212	root	209:212	root	209:212	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	33	theme	different	190:198	arg1	flower					230:235	flower	230:235	flower	230:235	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	33	theme	different	190:198	arg1	tissues					200:206	different tissues	190:206	different tissues (root, stem, leaf and flower)	190:236	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	2	34	theme	manihot	408:414	arg1	tissues					394:400	the different tissues	380:400	the different tissues of A. manihot	380:414	The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS.
28929656	6	35	from	various	1353:1359	arg1	tissues					1374:1380	different tissues	1364:1380	different tissues	1364:1380	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	4	36	contain	have	991:994	arg2	content					1008:1014	the highest content	996:1014	the highest content (29.88%) of cellulose	996:1036	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	36	contain	have	991:994	arg2	%					1022:1022	29.88%	1017:1022	29.88%	1017:1022	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	36	contain	have	991:994	arg1	roots					985:989	the roots	981:989	the roots	981:989	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	3	37	theme	resourceful	523:533	arg1	compositions					544:555	the resourceful chemical compositions	519:555	the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3'-O-glucoside, rutin and quercetin	519:701	The flowers are rich in the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3'-O-glucoside, rutin and quercetin.
28929656	6	38	from	tissues	1374:1380	arg1	various					1353:1359	various	1353:1359	various	1353:1359	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	2	39	theme	A.	405:406	arg1	manihot					408:414	A. manihot	405:414	A. manihot	405:414	The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS.
28929656	3	40	theme	chemical	535:542	arg1	compositions					544:555	the resourceful chemical compositions	519:555	the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3'-O-glucoside, rutin and quercetin	519:701	The flowers are rich in the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3'-O-glucoside, rutin and quercetin.
28929656	5	41	theme	highest	1207:1213	arg1	content					1215:1221	the highest content	1203:1221	the highest content of nucleosides (1.474 mg•g⁻¹)	1203:1251	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	6	42	theme	different	1364:1372	arg1	tissues					1374:1380	different tissues	1364:1380	different tissues	1364:1380	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	5	43	theme	nucleosides	1069:1079	arg1	Total					1039:1043	Total	1039:1043	Total of 21 amino acids and 9 nucleosides	1039:1079	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	4	44	theme	total	708:712	arg1	content					714:720	The total content	704:720	The total content of these flavonoids	704:740	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	44	theme	total	708:712	arg1	mg•g-1					752:757	25.450 mg•g-1	745:757	25.450 mg•g-1 in the flowers	745:772	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	45	theme	highest	1000:1006	arg1	content					1008:1014	the highest content	996:1014	the highest content (29.88%) of cellulose	996:1036	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	45	theme	highest	1000:1006	arg1	%					1022:1022	29.88%	1017:1022	29.88%	1017:1022	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	2	46	theme	different	384:392	arg1	tissues					394:400	the different tissues	380:400	the different tissues of A. manihot	380:414	The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS.
28929656	4	47	theme	polysaccharides	958:972	arg1	content					931:937	the highest content	919:937	the highest content(19.76%) of soluble polysaccharides	919:972	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	47	theme	polysaccharides	958:972	arg1	%					944:944	19.76%	939:944	19.76%	939:944	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	1	48	theme	utilizing	280:288	arg1	value					290:294	their utilizing value	274:294	their utilizing value	274:294	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	6	49	theme	chemical	1292:1299	arg1	compositions					1301:1312	the resourceful chemical compositions	1276:1312	the resourceful chemical compositions	1276:1312	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	0	50	theme	value	26:30	arg1	discussion					32:41	value discussion	26:41	value discussion	26:41	[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].
28929656	6	51	theme	manihot	1489:1495	arg1	plants					1497:1502	A. manihot plants	1486:1502	A. manihot plants	1486:1502	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	0	52	theme	chemical	55:62	arg1	composition					64:74	multiple chemical composition	46:74	multiple chemical composition	46:74	[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].
28929656	6	53	dep	results	1400:1406	arg1	provided					1408:1415	provided	1408:1415	results provided a scientific basis for the utilization and industrial development of A. manihot plants	1400:1502	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	4	54	from	celluloses	889:898	arg1	rich					853:856	rich	853:856	rich	853:856	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	54	from	celluloses	889:898	arg1	tissues					827:833	the other tissues.Different tissues	799:833	the other tissues.Different tissues of A. manihot	799:847	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	3	55	theme	flavonoids	560:569	arg1	compositions					544:555	the resourceful chemical compositions	519:555	the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3'-O-glucoside, rutin and quercetin	519:701	The flowers are rich in the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3'-O-glucoside, rutin and quercetin.
28929656	6	56	theme	A.	1486:1487	arg1	plants					1497:1502	A. manihot plants	1486:1502	A. manihot plants	1486:1502	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	0	57	theme	multiple	46:53	arg1	composition					64:74	multiple chemical composition	46:74	multiple chemical composition	46:74	[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].
28929656	6	58	theme	resourceful	1280:1290	arg1	compositions					1301:1312	the resourceful chemical compositions	1276:1312	the resourceful chemical compositions	1276:1312	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	5	59	theme	amino	1051:1055	arg1	acids					1057:1061	21 amino acids	1048:1061	21 amino acids	1048:1061	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	4	60	theme	other	803:807	arg1	rich					853:856	rich	853:856	rich	853:856	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	60	theme	other	803:807	arg1	tissues					827:833	the other tissues.Different tissues	799:833	the other tissues.Different tissues of A. manihot	799:847	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	2	61	theme	soluble	313:319	arg1	polysaccharides					321:335	soluble polysaccharides	313:335	soluble polysaccharides	313:335	The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS.
28929656	5	62	contain	have	1198:1201	arg2	content					1215:1221	the highest content	1203:1221	the highest content of nucleosides (1.474 mg•g⁻¹)	1203:1251	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	5	62	contain	have	1198:1201	arg1	the					1187:1189	the	1187:1189	the	1187:1189	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	6	63	theme	plants	1497:1502	arg1	utilization					1444:1454	utilization	1444:1454	utilization	1444:1454	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	6	63	theme	plants	1497:1502	arg1	development					1471:1481	industrial development	1460:1481	industrial development	1460:1481	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	4	64	from	mg•g-1	752:757	arg1	flowers					766:772	the flowers	762:772	the flowers	762:772	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	2	65	from	acids	371:375	arg1	tissues					394:400	the different tissues	380:400	the different tissues of A. manihot	380:414	The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS.
28929656	6	66	from	compositions	1301:1312	arg1	rich					1268:1271	rich	1268:1271	rich	1268:1271	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	6	67	theme	industrial	1460:1469	arg1	development					1471:1481	industrial development	1460:1481	industrial development	1460:1481	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	3	68	from	compositions	544:555	arg1	rich					511:514	rich	511:514	rich	511:514	The flowers are rich in the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3'-O-glucoside, rutin and quercetin.
28929656	3	68	from	compositions	544:555	arg1	flowers					499:505	The flowers	495:505	The flowers	495:505	The flowers are rich in the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3'-O-glucoside, rutin and quercetin.
28929656	4	69	from	polysaccharides	869:883	arg1	rich					853:856	rich	853:856	rich	853:856	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	69	from	polysaccharides	869:883	arg1	tissues					827:833	the other tissues.Different tissues	799:833	the other tissues.Different tissues of A. manihot	799:847	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	1	70	dep	tissues	200:206	arg1	leaf					221:224	leaf	221:224	leaf	221:224	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	70	dep	tissues	200:206	arg1	stem					215:218	stem	215:218	stem	215:218	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	70	dep	tissues	200:206	arg1	root					209:212	root	209:212	root	209:212	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	70	dep	tissues	200:206	arg1	tissues					200:206	different tissues	190:206	different tissues (root, stem, leaf and flower)	190:236	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	1	70	dep	tissues	200:206	arg1	flower					230:235	flower	230:235	flower	230:235	This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value.
28929656	6	71	dep	utilization	1444:1454	arg1	the					1440:1442	the	1440:1442	the	1440:1442	A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.
28929656	4	72	theme	cellulose	1028:1036	arg1	content					1008:1014	the highest content	996:1014	the highest content (29.88%) of cellulose	996:1036	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	4	72	theme	cellulose	1028:1036	arg1	%					1022:1022	29.88%	1017:1022	29.88%	1017:1022	The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose.
28929656	5	73	theme	amino	1154:1158	arg1	mg•g⁻¹					1172:1177	4.737 mg•g⁻¹	1166:1177	4.737 mg•g⁻¹	1166:1177	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	5	73	theme	amino	1154:1158	arg1	acids					1160:1164	amino acids	1154:1164	amino acids(4.737 mg•g⁻¹)	1154:1178	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	0	74	theme	composition	64:74	arg1	utilization					14:24	utilization	14:24	utilization	14:24	[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].
28929656	0	74	theme	composition	64:74	arg1	[Analysis					0:8	[Analysis	0:8	[Analysis	0:8	[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].
28929656	5	75	theme	acids	1057:1061	arg1	Total					1039:1043	Total	1039:1043	Total of 21 amino acids and 9 nucleosides	1039:1079	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
28929656	0	76	dep	[Analysis	0:8	arg1	discussion					32:41	value discussion	26:41	value discussion	26:41	[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].
28929656	5	77	theme	acids	1160:1164	arg1	content					1143:1149	the highest content	1131:1149	the highest content of amino acids(4.737 mg•g⁻¹)	1131:1178	Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹).
29253545	0	0	theme	calcium	85:91	arg1	pectate					93:99	calcium pectate	85:99	calcium pectate	85:99	Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.
29253545	5	1	with	beads	904:908	arg1	CP					915:916	CP	915:916	CP	915:916	In addition, it was found that it was relatively easier to form CaP beads with CP compared with AP.
29253545	1	2	theme	notable	149:155	arg1	ability					194:200	the ability to form gel	190:212	the ability to form gel	190:212	The most notable and unique property of pectin is the ability to form gel; thus, many biological applications of pectin are based on its gelation properties.
29253545	1	2	theme	notable	149:155	arg1	property					168:175	The most notable and unique property	140:175	The most notable and unique property of pectin	140:185	The most notable and unique property of pectin is the ability to form gel; thus, many biological applications of pectin are based on its gelation properties.
29253545	2	3	theme	function	448:455	arg1	properties					457:466	different gelling and function properties	426:466	different gelling and function properties	426:466	Pectin isolated from different plant cell walls may differ in molecular structure and distribution pattern, which may result in different gelling and function properties.
29253545	3	4	theme	citrus	631:636	arg1	CP					646:647	CP	646:647	CP	646:647	In this work, we investigated the chemical characteristics, gelation properties, and biological application of calcium pectate (CaP) prepared using apple (AP) or citrus pectin (CP).
29253545	3	4	theme	citrus	631:636	arg1	pectin					638:643	citrus pectin	631:643	citrus pectin (CP)	631:648	In this work, we investigated the chemical characteristics, gelation properties, and biological application of calcium pectate (CaP) prepared using apple (AP) or citrus pectin (CP).
29253545	4	5	theme	composition	787:797	arg1	proportion					799:808	the composition proportion	783:808	the composition proportion of single monosaccharide	783:833	These two types of pectins exhibited similar molecular parameters and glycosidic bone structure; however, there was a difference in the composition proportion of single monosaccharide.
29253545	7	6	contain	have	1049:1052	arg1	beads					1043:1047	CP-CaP beads	1036:1047	CP-CaP beads	1036:1047	The morphological study suggests that CP-CaP beads have a rough wrinkle structure on the surface, which might benefit mass transfer and cell proliferation.
29253545	7	6	contain	have	1049:1052	arg2	structure					1070:1078	a rough wrinkle structure	1054:1078	a rough wrinkle structure	1054:1078	The morphological study suggests that CP-CaP beads have a rough wrinkle structure on the surface, which might benefit mass transfer and cell proliferation.
29253545	2	7	theme	different	319:327	arg1	walls					340:344	different plant cell walls	319:344	different plant cell walls	319:344	Pectin isolated from different plant cell walls may differ in molecular structure and distribution pattern, which may result in different gelling and function properties.
29253545	1	8	theme	many	221:224	arg1	applications					237:248	many biological applications	221:248	many biological applications of pectin	221:258	The most notable and unique property of pectin is the ability to form gel; thus, many biological applications of pectin are based on its gelation properties.
29253545	8	9	theme	CP-CaP	1275:1280	arg1	beads					1282:1286	the AP-CaP and CP-CaP beads	1260:1286	beads	1282:1286	Moreover, although there are some differences in the viability and proliferation of cells encapsulated in the AP-CaP and CP-CaP beads, both can be used for cell encapsulation.
29253545	4	10	theme	glycosidic	721:730	arg1	structure					737:745	glycosidic bone structure	721:745	glycosidic bone structure	721:745	These two types of pectins exhibited similar molecular parameters and glycosidic bone structure; however, there was a difference in the composition proportion of single monosaccharide.
29253545	4	11	theme	single	813:818	arg1	monosaccharide					820:833	single monosaccharide	813:833	single monosaccharide	813:833	These two types of pectins exhibited similar molecular parameters and glycosidic bone structure; however, there was a difference in the composition proportion of single monosaccharide.
29253545	3	12	theme	chemical	503:510	arg1	characteristics					512:526	the chemical characteristics	499:526	the chemical characteristics	499:526	In this work, we investigated the chemical characteristics, gelation properties, and biological application of calcium pectate (CaP) prepared using apple (AP) or citrus pectin (CP).
29253545	4	13	theme	pectins	670:676	arg1	types					661:665	These two types	651:665	These two types of pectins	651:676	These two types of pectins exhibited similar molecular parameters and glycosidic bone structure; however, there was a difference in the composition proportion of single monosaccharide.
29253545	1	14	theme	biological	226:235	arg1	applications					237:248	many biological applications	221:248	many biological applications of pectin	221:258	The most notable and unique property of pectin is the ability to form gel; thus, many biological applications of pectin are based on its gelation properties.
29253545	0	15	theme	pectate	93:99	arg1	characteristics					18:32	chemical characteristics	9:32	chemical characteristics	9:32	Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.
29253545	0	15	theme	pectate	93:99	arg1	properties					44:53	gelation properties	35:53	gelation properties	35:53	Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.
29253545	0	15	theme	pectate	93:99	arg1	application					70:80	biological application	59:80	biological application of calcium pectate	59:99	Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.
29253545	2	16	attach	isolated	305:312	arg2	Pectin					298:303	Pectin	298:303	Pectin isolated from different plant cell walls	298:344	Pectin isolated from different plant cell walls may differ in molecular structure and distribution pattern, which may result in different gelling and function properties.
29253545	2	16	attach	isolated	305:312	arg1	walls					340:344	different plant cell walls	319:344	different plant cell walls	319:344	Pectin isolated from different plant cell walls may differ in molecular structure and distribution pattern, which may result in different gelling and function properties.
29253545	4	17	theme	similar	688:694	arg1	parameters					706:715	similar molecular parameters	688:715	similar molecular parameters	688:715	These two types of pectins exhibited similar molecular parameters and glycosidic bone structure; however, there was a difference in the composition proportion of single monosaccharide.
29253545	8	18	from	differences	1188:1198	arg1	proliferation					1221:1233	proliferation	1221:1233	proliferation	1221:1233	Moreover, although there are some differences in the viability and proliferation of cells encapsulated in the AP-CaP and CP-CaP beads, both can be used for cell encapsulation.
29253545	8	18	from	differences	1188:1198	arg1	viability					1207:1215	viability	1207:1215	viability	1207:1215	Moreover, although there are some differences in the viability and proliferation of cells encapsulated in the AP-CaP and CP-CaP beads, both can be used for cell encapsulation.
29253545	2	19	theme	plant	329:333	arg1	walls					340:344	different plant cell walls	319:344	different plant cell walls	319:344	Pectin isolated from different plant cell walls may differ in molecular structure and distribution pattern, which may result in different gelling and function properties.
29253545	1	20	theme	unique	161:166	arg1	ability					194:200	the ability to form gel	190:212	the ability to form gel	190:212	The most notable and unique property of pectin is the ability to form gel; thus, many biological applications of pectin are based on its gelation properties.
29253545	1	20	theme	unique	161:166	arg1	property					168:175	The most notable and unique property	140:175	The most notable and unique property of pectin	140:185	The most notable and unique property of pectin is the ability to form gel; thus, many biological applications of pectin are based on its gelation properties.
29253545	7	21	theme	morphological	1002:1014	arg1	study					1016:1020	The morphological study	998:1020	The morphological study	998:1020	The morphological study suggests that CP-CaP beads have a rough wrinkle structure on the surface, which might benefit mass transfer and cell proliferation.
29253545	8	22	used	used	1301:1304	arg2	both					1289:1292	both	1289:1292	both	1289:1292	Moreover, although there are some differences in the viability and proliferation of cells encapsulated in the AP-CaP and CP-CaP beads, both can be used for cell encapsulation.
29253545	5	23	theme	CaP	900:902	arg1	beads					904:908	CaP beads	900:908	CaP beads with CP	900:916	In addition, it was found that it was relatively easier to form CaP beads with CP compared with AP.
29253545	7	24	theme	mass	1116:1119	arg1	transfer					1121:1128	mass transfer	1116:1128	mass transfer	1116:1128	The morphological study suggests that CP-CaP beads have a rough wrinkle structure on the surface, which might benefit mass transfer and cell proliferation.
29253545	6	25	theme	binding	968:974	arg1	capability					976:985	a higher binding capability	959:985	a higher binding capability	959:985	Moreover, CP exhibited a higher binding capability with Ca2+.
29253545	5	26	located	found	856:860	arg2	it					849:850	it	849:850	it	849:850	In addition, it was found that it was relatively easier to form CaP beads with CP compared with AP.
29253545	5	26	located	found	856:860	arg1	addition					839:846	addition	839:846	addition	839:846	In addition, it was found that it was relatively easier to form CaP beads with CP compared with AP.
29253545	8	27	theme	AP-CaP	1264:1269	arg1	beads					1282:1286	the AP-CaP and CP-CaP beads	1260:1286	beads	1282:1286	Moreover, although there are some differences in the viability and proliferation of cells encapsulated in the AP-CaP and CP-CaP beads, both can be used for cell encapsulation.
29253545	2	28	theme	molecular	360:368	arg1	structure					370:378	molecular structure	360:378	molecular structure	360:378	Pectin isolated from different plant cell walls may differ in molecular structure and distribution pattern, which may result in different gelling and function properties.
29253545	1	29	theme	pectin	180:185	arg1	ability					194:200	the ability to form gel	190:212	the ability to form gel	190:212	The most notable and unique property of pectin is the ability to form gel; thus, many biological applications of pectin are based on its gelation properties.
29253545	1	29	theme	pectin	180:185	arg1	property					168:175	The most notable and unique property	140:175	The most notable and unique property of pectin	140:185	The most notable and unique property of pectin is the ability to form gel; thus, many biological applications of pectin are based on its gelation properties.
29253545	0	30	theme	characteristics	18:32	arg1	Study					0:4	Study	0:4	Study of chemical characteristics, gelation properties and biological application of calcium pectate	0:99	Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.
29253545	1	31	theme	pectin	253:258	arg1	applications					237:248	many biological applications	221:248	many biological applications of pectin	221:258	The most notable and unique property of pectin is the ability to form gel; thus, many biological applications of pectin are based on its gelation properties.
29253545	0	32	theme	citrus	125:130	arg1	pectin					132:137	citrus pectin	125:137	citrus pectin	125:137	Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.
29253545	8	33	dep	viability	1207:1215	arg1	the					1203:1205	the	1203:1205	the	1203:1205	Moreover, although there are some differences in the viability and proliferation of cells encapsulated in the AP-CaP and CP-CaP beads, both can be used for cell encapsulation.
29253545	0	34	theme	chemical	9:16	arg1	characteristics					18:32	chemical characteristics	9:32	chemical characteristics	9:32	Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.
29253545	3	35	theme	gelation	529:536	arg1	properties					538:547	gelation properties	529:547	gelation properties	529:547	In this work, we investigated the chemical characteristics, gelation properties, and biological application of calcium pectate (CaP) prepared using apple (AP) or citrus pectin (CP).
29253545	2	36	theme	distribution	384:395	arg1	pattern					397:403	distribution pattern	384:403	distribution pattern	384:403	Pectin isolated from different plant cell walls may differ in molecular structure and distribution pattern, which may result in different gelling and function properties.
29253545	0	37	theme	gelation	35:42	arg1	properties					44:53	gelation properties	35:53	gelation properties	35:53	Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.
29253545	6	38	theme	higher	961:966	arg1	capability					976:985	a higher binding capability	959:985	a higher binding capability	959:985	Moreover, CP exhibited a higher binding capability with Ca2+.
29253545	2	39	theme	gelling	436:442	arg1	properties					457:466	different gelling and function properties	426:466	different gelling and function properties	426:466	Pectin isolated from different plant cell walls may differ in molecular structure and distribution pattern, which may result in different gelling and function properties.
29253545	7	40	theme	rough	1056:1060	arg1	structure					1070:1078	a rough wrinkle structure	1054:1078	a rough wrinkle structure	1054:1078	The morphological study suggests that CP-CaP beads have a rough wrinkle structure on the surface, which might benefit mass transfer and cell proliferation.
29253545	7	41	theme	wrinkle	1062:1068	arg1	structure					1070:1078	a rough wrinkle structure	1054:1078	a rough wrinkle structure	1054:1078	The morphological study suggests that CP-CaP beads have a rough wrinkle structure on the surface, which might benefit mass transfer and cell proliferation.
29253545	2	42	theme	different	426:434	arg1	properties					457:466	different gelling and function properties	426:466	different gelling and function properties	426:466	Pectin isolated from different plant cell walls may differ in molecular structure and distribution pattern, which may result in different gelling and function properties.
29253545	0	43	theme	properties	44:53	arg1	Study					0:4	Study	0:4	Study of chemical characteristics, gelation properties and biological application of calcium pectate	0:99	Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.
29253545	1	44	theme	gelation	277:284	arg1	properties					286:295	its gelation properties	273:295	its gelation properties	273:295	The most notable and unique property of pectin is the ability to form gel; thus, many biological applications of pectin are based on its gelation properties.
29253545	4	45	theme	monosaccharide	820:833	arg1	proportion					799:808	the composition proportion	783:808	the composition proportion of single monosaccharide	783:833	These two types of pectins exhibited similar molecular parameters and glycosidic bone structure; however, there was a difference in the composition proportion of single monosaccharide.
29253545	3	46	theme	biological	554:563	arg1	application					565:575	biological application	554:575	biological application	554:575	In this work, we investigated the chemical characteristics, gelation properties, and biological application of calcium pectate (CaP) prepared using apple (AP) or citrus pectin (CP).
29253545	7	47	theme	CP-CaP	1036:1041	arg1	beads					1043:1047	CP-CaP beads	1036:1047	CP-CaP beads	1036:1047	The morphological study suggests that CP-CaP beads have a rough wrinkle structure on the surface, which might benefit mass transfer and cell proliferation.
29253545	8	48	theme	cell	1310:1313	arg1	encapsulation					1315:1327	cell encapsulation	1310:1327	cell encapsulation	1310:1327	Moreover, although there are some differences in the viability and proliferation of cells encapsulated in the AP-CaP and CP-CaP beads, both can be used for cell encapsulation.
29253545	4	49	theme	bone	732:735	arg1	structure					737:745	glycosidic bone structure	721:745	glycosidic bone structure	721:745	These two types of pectins exhibited similar molecular parameters and glycosidic bone structure; however, there was a difference in the composition proportion of single monosaccharide.
29253545	4	50	theme	molecular	696:704	arg1	parameters					706:715	similar molecular parameters	688:715	similar molecular parameters	688:715	These two types of pectins exhibited similar molecular parameters and glycosidic bone structure; however, there was a difference in the composition proportion of single monosaccharide.
29253545	8	51	theme	cells	1238:1242	arg1	proliferation					1221:1233	proliferation	1221:1233	proliferation	1221:1233	Moreover, although there are some differences in the viability and proliferation of cells encapsulated in the AP-CaP and CP-CaP beads, both can be used for cell encapsulation.
29253545	8	51	theme	cells	1238:1242	arg1	viability					1207:1215	viability	1207:1215	viability	1207:1215	Moreover, although there are some differences in the viability and proliferation of cells encapsulated in the AP-CaP and CP-CaP beads, both can be used for cell encapsulation.
29253545	0	52	theme	application	70:80	arg1	Study					0:4	Study	0:4	Study of chemical characteristics, gelation properties and biological application of calcium pectate	0:99	Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.
29253545	3	53	theme	calcium	580:586	arg1	pectate					588:594	calcium pectate	580:594	calcium pectate (CaP) prepared using apple (AP) or citrus pectin (CP)	580:648	In this work, we investigated the chemical characteristics, gelation properties, and biological application of calcium pectate (CaP) prepared using apple (AP) or citrus pectin (CP).
29253545	3	53	theme	calcium	580:586	arg1	CaP					597:599	CaP	597:599	CaP	597:599	In this work, we investigated the chemical characteristics, gelation properties, and biological application of calcium pectate (CaP) prepared using apple (AP) or citrus pectin (CP).
29253545	0	54	theme	biological	59:68	arg1	application					70:80	biological application	59:80	biological application of calcium pectate	59:99	Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.
29253545	3	55	theme	pectate	588:594	arg1	application					565:575	biological application	554:575	biological application	554:575	In this work, we investigated the chemical characteristics, gelation properties, and biological application of calcium pectate (CaP) prepared using apple (AP) or citrus pectin (CP).
29253545	3	55	theme	pectate	588:594	arg1	characteristics					512:526	the chemical characteristics	499:526	the chemical characteristics	499:526	In this work, we investigated the chemical characteristics, gelation properties, and biological application of calcium pectate (CaP) prepared using apple (AP) or citrus pectin (CP).
29253545	3	55	theme	pectate	588:594	arg1	properties					538:547	gelation properties	529:547	gelation properties	529:547	In this work, we investigated the chemical characteristics, gelation properties, and biological application of calcium pectate (CaP) prepared using apple (AP) or citrus pectin (CP).
29253545	2	56	theme	cell	335:338	arg1	walls					340:344	different plant cell walls	319:344	different plant cell walls	319:344	Pectin isolated from different plant cell walls may differ in molecular structure and distribution pattern, which may result in different gelling and function properties.
29253545	7	57	theme	cell	1134:1137	arg1	proliferation					1139:1151	cell proliferation	1134:1151	cell proliferation	1134:1151	The morphological study suggests that CP-CaP beads have a rough wrinkle structure on the surface, which might benefit mass transfer and cell proliferation.
24918655	4	0	theme	homogenous	734:743	arg1	solutions					753:761	homogenous ternary solutions	734:761	homogenous ternary solutions of nonelectrolytes	734:780	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	1	theme	other	914:918	arg1	one					920:922	the other one	910:922	the other one	910:922	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	8	2	theme	solution	1434:1441	arg1	C1					1454:1455	C1	1454:1455	C1	1454:1455	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	8	2	theme	solution	1434:1441	arg1	component					1443:1451	one solution component	1430:1451	one solution component (C1)	1430:1456	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	6	3	theme	K-K	1140:1142	arg1	method					1202:1207	a study method	1194:1207	a study method	1194:1207	The authors applied PNT formalism and K-K equations for ternary nonelectrotyle solutions as a study method.
24918655	6	3	theme	K-K	1140:1142	arg1	formalism					1126:1134	PNT formalism	1122:1134	PNT formalism	1122:1134	The authors applied PNT formalism and K-K equations for ternary nonelectrotyle solutions as a study method.
24918655	6	3	theme	K-K	1140:1142	arg1	equations					1144:1152	K-K equations	1140:1152	K-K equations	1140:1152	The authors applied PNT formalism and K-K equations for ternary nonelectrotyle solutions as a study method.
24918655	8	4	theme	other	1488:1492	arg1	one					1494:1496	the other one	1484:1496	the other one	1484:1496	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	5	5	theme	ethanol	1063:1069	arg1	solutions					1071:1079	aqueous glucose and ethanol solutions	1043:1079	aqueous glucose and ethanol solutions as a study material	1043:1099	MATERIAL AND METHODS The authors used celulose Nephrophan membrane of known transport parameters for aqueous glucose and ethanol solutions as a study material.
24918655	2	6	theme	BACKGROUND	155:164	arg1	PNT					200:202	PNT	200:202	PNT	200:202	BACKGROUND Peusner's network thermodynamics (PNT) allows symmetrical and/or hybrid transformation of Kedem-Katchalsky (K-K) equations to network form.
24918655	2	6	theme	BACKGROUND	155:164	arg1	thermodynamics					184:197	BACKGROUND Peusner's network thermodynamics	155:197	BACKGROUND Peusner's network thermodynamics (PNT)	155:203	BACKGROUND Peusner's network thermodynamics (PNT) allows symmetrical and/or hybrid transformation of Kedem-Katchalsky (K-K) equations to network form.
24918655	7	7	theme	dissolved	1316:1324	arg1	substances					1342:1351	two dissolved non-electrolyte substances	1312:1351	two dissolved non-electrolyte substances	1312:1351	RESULTS Hybrid network form of K-K equations was obtained for solutions that consist of a solvent and two dissolved non-electrolyte substances.
24918655	11	8	theme	solutions	2014:2022	arg1	composition					1999:2009	composition	1999:2009	composition	1999:2009	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	11	8	theme	solutions	2014:2022	arg1	concentration					1981:1993	concentration	1981:1993	concentration	1981:1993	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	9	9	theme	solute	1686:1691	arg1	permeability					1693:1704	solute permeability	1686:1704	solute permeability (ω)	1686:1708	These calculations were done using experimentally determined coefficients of reflection (σ), hydraulic (Lp) and solute permeability (ω).
24918655	9	9	theme	solute	1686:1691	arg1	ω					1707:1707	ω	1707:1707	ω	1707:1707	These calculations were done using experimentally determined coefficients of reflection (σ), hydraulic (Lp) and solute permeability (ω).
24918655	10	10	theme	K-K	1739:1741	arg1	equations					1743:1751	K-K equations	1739:1751	K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3})	1739:1808	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	11	11	from	alterations	1966:1976	arg1	composition					1999:2009	composition	1999:2009	composition	1999:2009	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	11	11	from	alterations	1966:1976	arg1	concentration					1981:1993	concentration	1981:1993	concentration	1981:1993	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	4	12	dep	i	712:712	arg1	}					727:727	j ∈ {1, 2, 3}	715:727	j ∈ {1, 2, 3}	715:727	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	10	13	theme	membrane	1853:1860	arg1	transport					1862:1870	membrane transport	1853:1870	membrane transport	1853:1870	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	6	14	theme	study	1196:1200	arg1	equations					1144:1152	K-K equations	1140:1152	K-K equations	1140:1152	The authors applied PNT formalism and K-K equations for ternary nonelectrotyle solutions as a study method.
24918655	6	14	theme	study	1196:1200	arg1	formalism					1126:1134	PNT formalism	1122:1134	PNT formalism	1122:1134	The authors applied PNT formalism and K-K equations for ternary nonelectrotyle solutions as a study method.
24918655	6	14	theme	study	1196:1200	arg1	method					1202:1207	a study method	1194:1207	a study method	1194:1207	The authors applied PNT formalism and K-K equations for ternary nonelectrotyle solutions as a study method.
24918655	7	15	dep	RESULTS	1210:1216	arg1	obtained					1259:1266	obtained	1259:1266	was obtained for solutions that consist of a solvent and two dissolved non-electrolyte substances	1255:1351	RESULTS Hybrid network form of K-K equations was obtained for solutions that consist of a solvent and two dissolved non-electrolyte substances.
24918655	4	16	theme	coefficients	814:825	arg1	Sij					827:829	coefficients Sij	814:829	coefficients Sij	814:829	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	7	17	theme	K-K	1241:1243	arg1	equations					1245:1253	K-K equations	1241:1253	K-K equations	1241:1253	RESULTS Hybrid network form of K-K equations was obtained for solutions that consist of a solvent and two dissolved non-electrolyte substances.
24918655	10	18	theme	Peusner	1766:1772	arg1	i					1792:1792	i	1792:1792	i	1792:1792	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	10	18	theme	Peusner	1766:1772	arg1	Sij					1787:1789	Peusner coefficients Sij	1766:1789	Peusner coefficients Sij (i, j ∈ {1, 2, 3})	1766:1808	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	4	19	from	dependences	799:809	arg1	concentration					839:851	mean concentration	834:851	mean concentration of one solution component (C1)	834:882	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	2	20	theme	hybrid	231:236	arg1	transformation					238:251	symmetrical and/or hybrid transformation	212:251	transformation	238:251	BACKGROUND Peusner's network thermodynamics (PNT) allows symmetrical and/or hybrid transformation of Kedem-Katchalsky (K-K) equations to network form.
24918655	3	21	dep	coefficients	562:573	arg1	Kij					542:544	Kij	542:544	Kij	542:544	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	21	dep	coefficients	562:573	arg1	Nij					537:539	Nij	537:539	Nij	537:539	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	21	dep	coefficients	562:573	arg1	Pij					549:551	Pij	549:551	Pij	549:551	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	21	dep	coefficients	562:573	arg1	Wij					527:529	Wij	527:529	Wij	527:529	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	21	dep	coefficients	562:573	arg1	coefficients					562:573	symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients	486:573	coefficients	562:573	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	4	22	theme	nonelectrolytes	766:780	arg1	solutions					753:761	homogenous ternary solutions	734:761	homogenous ternary solutions of nonelectrolytes	734:780	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	6	23	theme	nonelectrotyle	1166:1179	arg1	solutions					1181:1189	ternary nonelectrotyle solutions	1158:1189	ternary nonelectrotyle solutions	1158:1189	The authors applied PNT formalism and K-K equations for ternary nonelectrotyle solutions as a study method.
24918655	2	24	theme	symmetrical	212:222	arg1	transformation					238:251	symmetrical and/or hybrid transformation	212:251	transformation	238:251	BACKGROUND Peusner's network thermodynamics (PNT) allows symmetrical and/or hybrid transformation of Kedem-Katchalsky (K-K) equations to network form.
24918655	2	25	theme	K-K	274:276	arg1	equations					279:287	Kedem-Katchalsky (K-K) equations	256:287	Kedem-Katchalsky (K-K) equations	256:287	BACKGROUND Peusner's network thermodynamics (PNT) allows symmetrical and/or hybrid transformation of Kedem-Katchalsky (K-K) equations to network form.
24918655	7	26	theme	Hybrid	1218:1223	arg1	form					1233:1236	Hybrid network form	1218:1236	Hybrid network form of K-K equations	1218:1253	RESULTS Hybrid network form of K-K equations was obtained for solutions that consist of a solvent and two dissolved non-electrolyte substances.
24918655	4	27	theme	K-K	652:654	arg1	equations					656:664	network K-K equations	644:664	network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes	644:780	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	1	28	theme	polymeric	134:142	arg1	membrane					144:151	polymeric membrane	134:151	polymeric membrane	134:151	Evaluation of Sij Peusner's coefficients for polymeric membrane].
24918655	0	29	theme	non-electrolyte	60:74	arg1	solutions					76:84	ternary non-electrolyte solutions	52:84	ternary non-electrolyte solutions	52:84	[Network form of the Kedem-Katchalsky equations for ternary non-electrolyte solutions 7.
24918655	4	30	theme	component	869:877	arg1	concentration					839:851	mean concentration	834:851	mean concentration of one solution component (C1)	834:882	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	31	theme	hybrid	629:634	arg1	form					636:639	the hybrid form	625:639	the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes	625:780	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	10	32	theme	CONCLUSIONS	1711:1721	arg1	form					1731:1734	CONCLUSIONS Network form	1711:1734	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3})	1711:1808	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	10	33	theme	{	1799:1799	arg1	}					1807:1807	j ∈ {1, 2, 3}	1795:1807	j ∈ {1, 2, 3}	1795:1807	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	4	34	dep	constant	927:934	arg1	C2					937:938	C2	937:938	C2	937:938	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	34	dep	constant	927:934	arg1	constant					927:934	constant	927:934	constant	927:934	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	34	dep	constant	927:934	arg1	concentration					893:905	the concentration	889:905	the concentration of the other one	889:922	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	8	35	theme	Sij	1382:1384	arg1	Dependences					1354:1364	Dependences	1354:1364	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1)	1354:1456	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	11	36	theme	polymer	2039:2045	arg1	membrane					2047:2054	a polymer membrane	2037:2054	a polymer membrane	2037:2054	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	4	37	theme	coefficients	694:705	arg1	i					712:712	i	712:712	i	712:712	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	37	theme	coefficients	694:705	arg1	Sij					707:709	tensor Peusner coefficients Sij	679:709	tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes	679:780	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	10	38	theme	j	1795:1795	arg1	}					1807:1807	j ∈ {1, 2, 3}	1795:1807	j ∈ {1, 2, 3}	1795:1807	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	3	39	dep	symmetrical	486:496	arg1	Lij					506:508	Lij	506:508	Lij	506:508	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	39	dep	symmetrical	486:496	arg1	Rij					499:501	Rij	499:501	Rij	499:501	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	40	contain	contain	478:484	arg2	coefficients					562:573	symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients	486:573	coefficients	562:573	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	40	contain	contain	478:484	arg2	Nij					537:539	Nij	537:539	Nij	537:539	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	40	contain	contain	478:484	arg2	Wij					527:529	Wij	527:529	Wij	527:529	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	40	contain	contain	478:484	arg2	Kij					542:544	Kij	542:544	Kij	542:544	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	40	contain	contain	478:484	arg1	equations					463:471	network K-K equations	451:471	network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients	451:573	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	40	contain	contain	478:484	arg2	Pij					549:551	Pij	549:551	Pij	549:551	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	40	contain	contain	478:484	arg2	symmetrical					486:496	symmetrical	486:496	symmetrical	486:496	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	11	41	dep	coefficients	1888:1899	arg1	S21					1916:1918	S21	1916:1918	S21	1916:1918	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	11	41	dep	coefficients	1888:1899	arg1	S31					1931:1933	S31	1931:1933	S31	1931:1933	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	11	41	dep	coefficients	1888:1899	arg1	coefficients					1888:1899	coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33	1888:1946	coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33	1888:1946	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	11	41	dep	coefficients	1888:1899	arg1	S11					1901:1903	S11	1901:1903	S11	1901:1903	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	11	41	dep	coefficients	1888:1899	arg1	S32					1936:1938	S32	1936:1938	S32	1936:1938	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	11	41	dep	coefficients	1888:1899	arg1	S12					1906:1908	S12	1906:1908	S12	1906:1908	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	11	41	dep	coefficients	1888:1899	arg1	S22					1921:1923	S22	1921:1923	S22	1921:1923	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	11	41	dep	coefficients	1888:1899	arg1	S33					1944:1946	S33	1944:1946	S33	1944:1946	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	11	41	dep	coefficients	1888:1899	arg1	S13					1911:1913	S13	1911:1913	S13	1911:1913	We showed that coefficients S11, S12, S13, S21, S22, S23, S31, S32 and S33 were sensitive to alterations in concentration and composition of solutions separated by a polymer membrane.
24918655	9	42	theme	determined	1624:1633	arg1	coefficients					1635:1646	experimentally determined coefficients	1609:1646	experimentally determined coefficients of reflection (σ), hydraulic (Lp) and solute permeability (ω)	1609:1708	These calculations were done using experimentally determined coefficients of reflection (σ), hydraulic (Lp) and solute permeability (ω).
24918655	8	43	theme	j	1390:1390	arg1	}					1402:1402	j ∈ {1, 2, 3}	1390:1402	j ∈ {1, 2, 3}	1390:1402	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	3	44	theme	six	431:433	arg1	hybrid					435:440	hybrid	435:440	hybrid	435:440	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	0	45	theme	[Network	0:7	arg1	form					9:12	[Network form	0:12	[Network form of the Kedem-Katchalsky equations for ternary non-electrolyte solutions	0:84	[Network form of the Kedem-Katchalsky equations for ternary non-electrolyte solutions 7.
24918655	5	46	theme	parameters	1028:1037	arg1	membrane					1000:1007	Nephrophan membrane	989:1007	Nephrophan membrane of known transport parameters	989:1037	MATERIAL AND METHODS The authors used celulose Nephrophan membrane of known transport parameters for aqueous glucose and ethanol solutions as a study material.
24918655	0	47	dep	7	86:86	arg1	form					9:12	[Network form	0:12	[Network form of the Kedem-Katchalsky equations for ternary non-electrolyte solutions	0:84	[Network form of the Kedem-Katchalsky equations for ternary non-electrolyte solutions 7.
24918655	3	48	theme	nonelec-metrolyte	371:387	arg1	substances					389:398	two soluble nonelec-metrolyte substances	359:398	two soluble nonelec-metrolyte substances	359:398	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	8	49	theme	{	1394:1394	arg1	}					1402:1402	j ∈ {1, 2, 3}	1390:1402	j ∈ {1, 2, 3}	1390:1402	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	4	50	theme	tensor	679:684	arg1	i					712:712	i	712:712	i	712:712	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	50	theme	tensor	679:684	arg1	Sij					707:709	tensor Peusner coefficients Sij	679:709	tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes	679:780	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	3	51	theme	equations	463:471	arg1	forms					442:446	two symmetrical and six hybrid forms	411:446	two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients	411:573	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	5	52	theme	aqueous	1043:1049	arg1	glucose					1051:1057	aqueous glucose	1043:1057	aqueous glucose	1043:1057	MATERIAL AND METHODS The authors used celulose Nephrophan membrane of known transport parameters for aqueous glucose and ethanol solutions as a study material.
24918655	3	53	theme	hybrid	514:519	arg1	Kij					542:544	Kij	542:544	Kij	542:544	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	53	theme	hybrid	514:519	arg1	Nij					537:539	Nij	537:539	Nij	537:539	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	53	theme	hybrid	514:519	arg1	Pij					549:551	Pij	549:551	Pij	549:551	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	53	theme	hybrid	514:519	arg1	Wij					527:529	Wij	527:529	Wij	527:529	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	53	theme	hybrid	514:519	arg1	coefficients					562:573	symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients	486:573	coefficients	562:573	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	54	theme	symmetrical	415:425	arg1	forms					442:446	two symmetrical and six hybrid forms	411:446	two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients	411:573	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	10	55	theme	research	1830:1837	arg1	tool					1839:1842	a novel research tool	1822:1842	a novel research tool to study membrane transport	1822:1870	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	2	56	theme	network	176:182	arg1	PNT					200:202	PNT	200:202	PNT	200:202	BACKGROUND Peusner's network thermodynamics (PNT) allows symmetrical and/or hybrid transformation of Kedem-Katchalsky (K-K) equations to network form.
24918655	2	56	theme	network	176:182	arg1	thermodynamics					184:197	BACKGROUND Peusner's network thermodynamics	155:197	BACKGROUND Peusner's network thermodynamics (PNT)	155:203	BACKGROUND Peusner's network thermodynamics (PNT) allows symmetrical and/or hybrid transformation of Kedem-Katchalsky (K-K) equations to network form.
24918655	3	57	theme	network	451:457	arg1	equations					463:471	network K-K equations	451:471	network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients	451:573	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	0	58	theme	Kedem-Katchalsky	21:36	arg1	equations					38:46	the Kedem-Katchalsky equations	17:46	the Kedem-Katchalsky equations for ternary non-electrolyte solutions	17:84	[Network form of the Kedem-Katchalsky equations for ternary non-electrolyte solutions 7.
24918655	5	59	theme	known	1012:1016	arg1	parameters					1028:1037	known transport parameters	1012:1037	known transport parameters	1012:1037	MATERIAL AND METHODS The authors used celulose Nephrophan membrane of known transport parameters for aqueous glucose and ethanol solutions as a study material.
24918655	10	60	theme	novel	1824:1828	arg1	tool					1839:1842	a novel research tool	1822:1842	a novel research tool to study membrane transport	1822:1870	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	4	61	theme	∈	717:717	arg1	}					727:727	j ∈ {1, 2, 3}	715:727	j ∈ {1, 2, 3}	715:727	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	10	62	theme	coefficients	1774:1785	arg1	i					1792:1792	i	1792:1792	i	1792:1792	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	10	62	theme	coefficients	1774:1785	arg1	Sij					1787:1789	Peusner coefficients Sij	1766:1789	Peusner coefficients Sij (i, j ∈ {1, 2, 3})	1766:1808	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	4	63	theme	one	920:922	arg1	concentration					893:905	the concentration	889:905	the concentration of the other one	889:922	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	63	theme	one	920:922	arg1	constant					927:934	constant	927:934	constant	927:934	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	63	theme	one	920:922	arg1	C2					937:938	C2	937:938	C2	937:938	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	8	64	gly	homogeneity	1548:1558	arg1	solutions					1563:1571	solutions	1563:1571	solutions	1563:1571	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	7	65	theme	non-electrolyte	1326:1340	arg1	substances					1342:1351	two dissolved non-electrolyte substances	1312:1351	two dissolved non-electrolyte substances	1312:1351	RESULTS Hybrid network form of K-K equations was obtained for solutions that consist of a solvent and two dissolved non-electrolyte substances.
24918655	2	66	theme	network	292:298	arg1	form					300:303	network form	292:303	network form	292:303	BACKGROUND Peusner's network thermodynamics (PNT) allows symmetrical and/or hybrid transformation of Kedem-Katchalsky (K-K) equations to network form.
24918655	4	67	theme	ternary	745:751	arg1	solutions					753:761	homogenous ternary solutions	734:761	homogenous ternary solutions of nonelectrolytes	734:780	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	8	68	theme	component	1443:1451	arg1	concentration					1413:1425	mean concentration	1408:1425	mean concentration of one solution component (C1)	1408:1456	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	6	69	theme	PNT	1122:1124	arg1	equations					1144:1152	K-K equations	1140:1152	K-K equations	1140:1152	The authors applied PNT formalism and K-K equations for ternary nonelectrotyle solutions as a study method.
24918655	6	69	theme	PNT	1122:1124	arg1	formalism					1126:1134	PNT formalism	1122:1134	PNT formalism	1122:1134	The authors applied PNT formalism and K-K equations for ternary nonelectrotyle solutions as a study method.
24918655	6	69	theme	PNT	1122:1124	arg1	method					1202:1207	a study method	1194:1207	a study method	1194:1207	The authors applied PNT formalism and K-K equations for ternary nonelectrotyle solutions as a study method.
24918655	2	70	theme	equations	279:287	arg1	transformation					238:251	symmetrical and/or hybrid transformation	212:251	transformation	238:251	BACKGROUND Peusner's network thermodynamics (PNT) allows symmetrical and/or hybrid transformation of Kedem-Katchalsky (K-K) equations to network form.
24918655	8	71	theme	one	1494:1496	arg1	C2					1510:1511	constant C2	1501:1511	constant C2	1501:1511	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	8	71	theme	one	1494:1496	arg1	concentration					1467:1479	the concentration	1463:1479	the concentration of the other one	1463:1496	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	9	72	theme	permeability	1693:1704	arg1	coefficients					1635:1646	experimentally determined coefficients	1609:1646	experimentally determined coefficients of reflection (σ), hydraulic (Lp) and solute permeability (ω)	1609:1708	These calculations were done using experimentally determined coefficients of reflection (σ), hydraulic (Lp) and solute permeability (ω).
24918655	8	73	theme	constant	1501:1508	arg1	C2					1510:1511	constant C2	1501:1511	constant C2	1501:1511	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	8	73	theme	constant	1501:1508	arg1	concentration					1467:1479	the concentration	1463:1479	the concentration of the other one	1463:1496	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	4	74	theme	study	603:607	arg1	aim					591:593	The aim	587:593	The aim of this study	587:607	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	10	75	theme	Network	1723:1729	arg1	form					1731:1734	CONCLUSIONS Network form	1711:1734	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3})	1711:1808	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	7	76	theme	equations	1245:1253	arg1	form					1233:1236	Hybrid network form	1218:1236	Hybrid network form of K-K equations	1218:1253	RESULTS Hybrid network form of K-K equations was obtained for solutions that consist of a solvent and two dissolved non-electrolyte substances.
24918655	3	77	theme	homogenous	310:319	arg1	solutions					321:329	homogenous solutions	310:329	homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances	310:398	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	10	78	theme	equations	1743:1751	arg1	form					1731:1734	CONCLUSIONS Network form	1711:1734	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3})	1711:1808	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	6	79	theme	ternary	1158:1164	arg1	solutions					1181:1189	ternary nonelectrotyle solutions	1158:1189	ternary nonelectrotyle solutions	1158:1189	The authors applied PNT formalism and K-K equations for ternary nonelectrotyle solutions as a study method.
24918655	3	80	theme	Peusner	554:560	arg1	Kij					542:544	Kij	542:544	Kij	542:544	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	80	theme	Peusner	554:560	arg1	Nij					537:539	Nij	537:539	Nij	537:539	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	80	theme	Peusner	554:560	arg1	Pij					549:551	Pij	549:551	Pij	549:551	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	80	theme	Peusner	554:560	arg1	Wij					527:529	Wij	527:529	Wij	527:529	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	3	80	theme	Peusner	554:560	arg1	coefficients					562:573	symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients	486:573	coefficients	562:573	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	8	81	theme	homogeneity	1548:1558	arg1	conditions					1534:1543	conditions	1534:1543	conditions of homogeneity of solutions	1534:1571	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	10	82	dep	i	1792:1792	arg1	}					1807:1807	j ∈ {1, 2, 3}	1795:1807	j ∈ {1, 2, 3}	1795:1807	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	7	83	theme	network	1225:1231	arg1	form					1233:1236	Hybrid network form	1218:1236	Hybrid network form of K-K equations	1218:1253	RESULTS Hybrid network form of K-K equations was obtained for solutions that consist of a solvent and two dissolved non-electrolyte substances.
24918655	4	84	theme	network	644:650	arg1	equations					656:664	network K-K equations	644:664	network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes	644:780	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	2	85	theme	Kedem-Katchalsky	256:271	arg1	equations					279:287	Kedem-Katchalsky (K-K) equations	256:287	Kedem-Katchalsky (K-K) equations	256:287	BACKGROUND Peusner's network thermodynamics (PNT) allows symmetrical and/or hybrid transformation of Kedem-Katchalsky (K-K) equations to network form.
24918655	8	86	theme	solutions	1563:1571	arg1	homogeneity					1548:1558	homogeneity	1548:1558	homogeneity of solutions	1548:1571	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	8	87	theme	coefficients	1369:1380	arg1	Sij					1382:1384	coefficients Sij	1369:1384	coefficients Sij (i, j ∈ {1, 2, 3})	1369:1403	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	8	87	theme	coefficients	1369:1380	arg1	i					1387:1387	i	1387:1387	i	1387:1387	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	4	88	theme	Sij	827:829	arg1	dependences					799:809	dependences	799:809	dependences of coefficients Sij on mean concentration of one solution component (C1)	799:882	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	89	theme	solution	860:867	arg1	C1					880:881	C1	880:881	C1	880:881	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	89	theme	solution	860:867	arg1	component					869:877	one solution component	856:877	one solution component (C1)	856:882	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	10	90	theme	∈	1797:1797	arg1	}					1807:1807	j ∈ {1, 2, 3}	1795:1807	j ∈ {1, 2, 3}	1795:1807	CONCLUSIONS Network form of K-K equations that include Peusner coefficients Sij (i, j ∈ {1, 2, 3}) constitutes a novel research tool to study membrane transport.
24918655	8	91	from	Dependences	1354:1364	arg1	concentration					1413:1425	mean concentration	1408:1425	mean concentration of one solution component (C1)	1408:1456	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	5	92	theme	Nephrophan	989:998	arg1	membrane					1000:1007	Nephrophan membrane	989:1007	Nephrophan membrane of known transport parameters	989:1037	MATERIAL AND METHODS The authors used celulose Nephrophan membrane of known transport parameters for aqueous glucose and ethanol solutions as a study material.
24918655	3	93	theme	soluble	363:369	arg1	substances					389:398	two soluble nonelec-metrolyte substances	359:398	two soluble nonelec-metrolyte substances	359:398	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	4	94	theme	Peusner	686:692	arg1	i					712:712	i	712:712	i	712:712	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	94	theme	Peusner	686:692	arg1	Sij					707:709	tensor Peusner coefficients Sij	679:709	tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes	679:780	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	4	95	theme	mean	834:837	arg1	concentration					839:851	mean concentration	834:851	mean concentration of one solution component (C1)	834:882	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	8	96	theme	∈	1392:1392	arg1	}					1402:1402	j ∈ {1, 2, 3}	1390:1402	j ∈ {1, 2, 3}	1390:1402	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	4	97	theme	equations	656:664	arg1	form					636:639	the hybrid form	625:639	the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes	625:780	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	3	98	theme	hybrid	435:440	arg1	forms					442:446	two symmetrical and six hybrid forms	411:446	two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients	411:573	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	1	99	theme	Sij	103:105	arg1	coefficients					117:128	Sij Peusner's coefficients	103:128	Sij Peusner's coefficients	103:128	Evaluation of Sij Peusner's coefficients for polymeric membrane].
24918655	0	100	theme	equations	38:46	arg1	form					9:12	[Network form	0:12	[Network form of the Kedem-Katchalsky equations for ternary non-electrolyte solutions	0:84	[Network form of the Kedem-Katchalsky equations for ternary non-electrolyte solutions 7.
24918655	5	101	theme	glucose	1051:1057	arg1	solutions					1071:1079	aqueous glucose and ethanol solutions	1043:1079	aqueous glucose and ethanol solutions as a study material	1043:1099	MATERIAL AND METHODS The authors used celulose Nephrophan membrane of known transport parameters for aqueous glucose and ethanol solutions as a study material.
24918655	4	102	theme	{	719:719	arg1	}					727:727	j ∈ {1, 2, 3}	715:727	j ∈ {1, 2, 3}	715:727	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	3	103	theme	K-K	459:461	arg1	equations					463:471	network K-K equations	451:471	network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients	451:573	For homogenous solutions that consist of solvent and two soluble nonelec-metrolyte substances, there are two symmetrical and six hybrid forms of network K-K equations that contain symmetrical (Rij or Lij) or hybrid (Hij, Wij, Sij, Nij, Kij or Pij) Peusner coefficients.
24918655	0	104	theme	ternary	52:58	arg1	solutions					76:84	ternary non-electrolyte solutions	52:84	ternary non-electrolyte solutions	52:84	[Network form of the Kedem-Katchalsky equations for ternary non-electrolyte solutions 7.
24918655	4	105	dep	OBJECTIVES	576:585	arg1	is					609:610	is	609:610	is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2)	609:939	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	9	106	theme	reflection	1651:1660	arg1	σ					1663:1663	reflection (σ)	1651:1664	reflection (σ)	1651:1664	These calculations were done using experimentally determined coefficients of reflection (σ), hydraulic (Lp) and solute permeability (ω).
24918655	5	107	dep	MATERIAL	942:949	arg1	authors					967:973	The authors	963:973	The authors used	963:978	MATERIAL AND METHODS The authors used celulose Nephrophan membrane of known transport parameters for aqueous glucose and ethanol solutions as a study material.
24918655	5	108	theme	transport	1018:1026	arg1	parameters					1028:1037	known transport parameters	1012:1037	known transport parameters	1012:1037	MATERIAL AND METHODS The authors used celulose Nephrophan membrane of known transport parameters for aqueous glucose and ethanol solutions as a study material.
24918655	9	109	theme	σ	1663:1663	arg1	coefficients					1635:1646	experimentally determined coefficients	1609:1646	experimentally determined coefficients of reflection (σ), hydraulic (Lp) and solute permeability (ω)	1609:1708	These calculations were done using experimentally determined coefficients of reflection (σ), hydraulic (Lp) and solute permeability (ω).
24918655	8	110	theme	mean	1408:1411	arg1	concentration					1413:1425	mean concentration	1408:1425	mean concentration of one solution component (C1)	1408:1456	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
24918655	5	111	theme	study	1086:1090	arg1	material					1092:1099	a study material	1084:1099	a study material	1084:1099	MATERIAL AND METHODS The authors used celulose Nephrophan membrane of known transport parameters for aqueous glucose and ethanol solutions as a study material.
24918655	9	112	theme	hydraulic	1667:1675	arg1	coefficients					1635:1646	experimentally determined coefficients	1609:1646	experimentally determined coefficients of reflection (σ), hydraulic (Lp) and solute permeability (ω)	1609:1708	These calculations were done using experimentally determined coefficients of reflection (σ), hydraulic (Lp) and solute permeability (ω).
24918655	4	113	theme	j	715:715	arg1	}					727:727	j ∈ {1, 2, 3}	715:727	j ∈ {1, 2, 3}	715:727	OBJECTIVES The aim of this study is to introduce the hybrid form of network K-K equations that include tensor Peusner coefficients Sij (i, j ∈ {1, 2, 3}) for homogenous ternary solutions of nonelectrolytes and to calculate dependences of coefficients Sij on mean concentration of one solution component (C1) when the concentration of the other one is constant (C2).
24918655	8	114	dep	i	1387:1387	arg1	}					1402:1402	j ∈ {1, 2, 3}	1390:1402	j ∈ {1, 2, 3}	1390:1402	Dependences of coefficients Sij (i, j ∈ {1, 2, 3}) on mean concentration of one solution component (C1) when the concentration of the other one is constant C2, were calculated for conditions of homogeneity of solutions.
28863216	10	0	from	present	1559:1565	arg1	rest					1574:1577	the rest	1570:1577	the rest of the corneal epithelium	1570:1603	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	2	1	theme	self-renewal	307:318	arg1	capacity					295:302	a high capacity	288:302	a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation	288:397	LSCs have a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation.
28863216	10	2	theme	LSC	1663:1665	arg1	niche					1667:1671	the LSC niche	1659:1671	the LSC niche	1659:1671	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	10	3	theme	epithelium	1594:1603	arg1	rest					1574:1577	the rest	1570:1577	the rest of the corneal epithelium	1570:1603	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	8	4	theme	HAS3-/-	1177:1183	arg1	mice					1214:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice	1169:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice	1169:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice have delayed wound healing and increased inflammation after injury.
28863216	6	5	dep	LSC	1035:1037	arg1	differentiation					1039:1053	differentiation	1039:1053	differentiation	1039:1053	Wild-type and HAS and TSG-6 knockout mice-HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, TSG-6-/--were used to determine the importance of the HA niche in LSC differentiation and specification.
28863216	6	5	dep	LSC	1035:1037	arg1	specification					1059:1071	specification	1059:1071	specification	1059:1071	Wild-type and HAS and TSG-6 knockout mice-HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, TSG-6-/--were used to determine the importance of the HA niche in LSC differentiation and specification.
28863216	5	6	theme	hyaluronan	796:805	arg1	HASs					818:821	HASs	818:821	HASs	818:821	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	6	theme	hyaluronan	796:805	arg1	synthases					807:815	hyaluronan synthases	796:815	hyaluronan synthases (HASs)	796:822	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	10	7	theme	matrix	1645:1650	arg1	disruption					1614:1623	the disruption	1610:1623	the disruption of this specific HA matrix within the LSC niche	1610:1671	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	1	8	theme	basal	167:171	arg1	layer					173:177	the basal layer	163:177	the basal layer of the corneal epithelium in the corneal limbus	163:225	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	10	9	theme	specific	1633:1640	arg1	matrix					1645:1650	this specific HA matrix	1628:1650	this specific HA matrix	1628:1650	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	1	10	theme	epithelial	122:131	arg1	LSCs					145:148	LSCs	145:148	LSCs	145:148	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	1	10	theme	epithelial	122:131	arg1	cells					138:142	Purpose Limbal epithelial stem cells	107:142	Purpose Limbal epithelial stem cells (LSCs)	107:149	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	8	11	theme	HAS1-/-	1169:1175	arg1	mice					1214:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice	1169:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice	1169:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice have delayed wound healing and increased inflammation after injury.
28863216	3	12	theme	stem	544:547	arg1	niche					554:558	the specific stem cell niche	531:558	the specific stem cell niche	531:558	To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function.
28863216	1	13	theme	corneal	186:192	arg1	epithelium					194:203	the corneal epithelium	182:203	the corneal epithelium	182:203	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	4	14	theme	extracellular	651:663	arg1	matrix					665:670	the extracellular matrix	647:670	the extracellular matrix	647:670	Our work investigates the composition of the extracellular matrix in the LSC niche and how it regulates LSC differentiation and function.
28863216	1	15	from	layer	173:177	arg1	located					152:158	located	152:158	located	152:158	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	1	15	from	layer	173:177	arg1	limbus					220:225	the corneal limbus	208:225	the corneal limbus	208:225	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	9	16	theme	compensatory	1384:1395	arg1	mechanism					1397:1405	a compensatory mechanism	1382:1405	a compensatory mechanism	1382:1405	Interestingly, upon insult the HAS knock-out mice up-regulate HA throughout the cornea through a compensatory mechanism, and in turn this alters LSC and epithelial cell specification.
28863216	8	17	theme	HAS2Δ/ΔCorEpi	1186:1198	arg1	mice					1214:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice	1169:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice	1169:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice have delayed wound healing and increased inflammation after injury.
28863216	10	18	theme	corneal	1694:1700	arg1	regeneration					1713:1724	compromised corneal epithelial regeneration	1682:1724	compromised corneal epithelial regeneration	1682:1724	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	5	19	theme	following	850:858	arg1	HAS2					879:882	HAS2	879:882	HAS2	879:882	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	19	theme	following	850:858	arg1	HAS3					889:892	HAS3	889:892	HAS3	889:892	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	19	theme	following	850:858	arg1	HAS1					873:876	HAS1	873:876	HAS1	873:876	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	19	theme	following	850:858	arg1	types					866:870	the following three types	846:870	the following three types: HAS1, HAS2, and HAS3	846:892	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	1	20	from	located	152:158	arg1	layer					173:177	the basal layer	163:177	the basal layer of the corneal epithelium in the corneal limbus	163:225	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	0	21	from	Microenvironment	16:31	arg1	Niche					57:61	the Limbal Stem Cell Niche	36:61	the Limbal Stem Cell Niche	36:61	Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation.
28863216	10	22	attach	present	1559:1565	arg2	that					1554:1557	that	1554:1557	that	1554:1557	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	10	22	attach	present	1559:1565	arg1	rest					1574:1577	the rest	1570:1577	the rest of the corneal epithelium	1570:1603	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	0	23	theme	Limbal	73:78	arg1	Differentiation					90:104	Limbal Stem Cell Differentiation	73:104	Limbal Stem Cell Differentiation	73:104	Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation.
28863216	6	24	dep	Wild-type	895:903	arg1	HAS3-/-					945:951	HAS3-/-	945:951	HAS3-/-	945:951	Wild-type and HAS and TSG-6 knockout mice-HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, TSG-6-/--were used to determine the importance of the HA niche in LSC differentiation and specification.
28863216	7	25	dep	Results	1074:1080	arg1	demonstrate					1091:1101	demonstrate	1091:1101	demonstrate that the LSC niche is composed of a HA rich extracellular matrix	1091:1166	Results Our data demonstrate that the LSC niche is composed of a HA rich extracellular matrix.
28863216	0	26	theme	Cell	85:88	arg1	Differentiation					90:104	Limbal Stem Cell Differentiation	73:104	Limbal Stem Cell Differentiation	73:104	Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation.
28863216	4	27	theme	LSC	710:712	arg1	differentiation					714:728	LSC differentiation	710:728	LSC differentiation	710:728	Our work investigates the composition of the extracellular matrix in the LSC niche and how it regulates LSC differentiation and function.
28863216	3	28	theme	limited	409:415	arg1	research					417:424	limited research	409:424	limited research	409:424	To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function.
28863216	9	29	theme	epithelial	1440:1449	arg1	specification					1456:1468	epithelial cell specification	1440:1468	epithelial cell specification	1440:1468	Interestingly, upon insult the HAS knock-out mice up-regulate HA throughout the cornea through a compensatory mechanism, and in turn this alters LSC and epithelial cell specification.
28863216	4	30	theme	LSC	679:681	arg1	niche					683:687	the LSC niche	675:687	the LSC niche	675:687	Our work investigates the composition of the extracellular matrix in the LSC niche and how it regulates LSC differentiation and function.
28863216	2	31	theme	error-free	349:358	arg1	proliferation					360:372	error-free proliferation	349:372	error-free proliferation	349:372	LSCs have a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation.
28863216	0	32	theme	Hyaluronan	0:9	arg1	Microenvironment					16:31	Hyaluronan Rich Microenvironment	0:31	Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche	0:61	Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation.
28863216	3	33	theme	cell	549:552	arg1	niche					554:558	the specific stem cell niche	531:558	the specific stem cell niche	531:558	To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function.
28863216	1	34	theme	Purpose	107:113	arg1	LSCs					145:148	LSCs	145:148	LSCs	145:148	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	1	34	theme	Purpose	107:113	arg1	cells					138:142	Purpose Limbal epithelial stem cells	107:142	Purpose Limbal epithelial stem cells (LSCs)	107:149	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	9	35	theme	knock-out	1322:1330	arg1	mice					1332:1335	knock-out mice	1322:1335	knock-out mice	1322:1335	Interestingly, upon insult the HAS knock-out mice up-regulate HA throughout the cornea through a compensatory mechanism, and in turn this alters LSC and epithelial cell specification.
28863216	0	36	theme	Stem	47:50	arg1	Niche					57:61	the Limbal Stem Cell Niche	36:61	the Limbal Stem Cell Niche	36:61	Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation.
28863216	6	37	theme	HA	1023:1024	arg1	niche					1026:1030	the HA niche	1019:1030	the HA niche	1019:1030	Wild-type and HAS and TSG-6 knockout mice-HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, TSG-6-/--were used to determine the importance of the HA niche in LSC differentiation and specification.
28863216	2	38	theme	high	290:293	arg1	capacity					295:302	a high capacity	288:302	a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation	288:397	LSCs have a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation.
28863216	1	39	theme	epithelium	194:203	arg1	layer					173:177	the basal layer	163:177	the basal layer of the corneal epithelium in the corneal limbus	163:225	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	3	40	theme	cell	486:489	arg1	niche					491:495	the limbal stem cell niche	470:495	the limbal stem cell niche	470:495	To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function.
28863216	7	41	theme	rich	1142:1145	arg1	matrix					1161:1166	a HA rich extracellular matrix	1137:1166	a HA rich extracellular matrix	1137:1166	Results Our data demonstrate that the LSC niche is composed of a HA rich extracellular matrix.
28863216	7	42	theme	LSC	1112:1114	arg1	niche					1116:1120	the LSC niche	1108:1120	the LSC niche	1108:1120	Results Our data demonstrate that the LSC niche is composed of a HA rich extracellular matrix.
28863216	3	43	theme	limbal	474:479	arg1	niche					491:495	the limbal stem cell niche	470:495	the limbal stem cell niche	470:495	To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function.
28863216	10	44	from	rest	1574:1577	arg1	present					1559:1565	present	1559:1565	present	1559:1565	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	10	45	theme	Conclusions	1471:1481	arg1	niche					1491:1495	Conclusions The LSC niche	1471:1495	Conclusions The LSC niche	1471:1495	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	1	46	theme	located	152:158	arg1	LSCs					145:148	LSCs	145:148	LSCs	145:148	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	1	46	theme	located	152:158	arg1	cells					138:142	Purpose Limbal epithelial stem cells	107:142	Purpose Limbal epithelial stem cells (LSCs)	107:149	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	10	47	theme	LSC	1487:1489	arg1	niche					1491:1495	Conclusions The LSC niche	1471:1495	Conclusions The LSC niche	1471:1495	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	10	48	theme	HA	1642:1643	arg1	matrix					1645:1650	this specific HA matrix	1628:1650	this specific HA matrix	1628:1650	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	8	49	theme	TSG-6-/-	1205:1212	arg1	mice					1214:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice	1169:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice	1169:1217	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice have delayed wound healing and increased inflammation after injury.
28863216	10	50	theme	specialized	1514:1524	arg1	matrix					1529:1534	a specialized HA matrix	1512:1534	a specialized HA matrix that differs from that present in the rest of the corneal epithelium	1512:1603	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	3	51	theme	specific	535:542	arg1	niche					554:558	the specific stem cell niche	531:558	the specific stem cell niche	531:558	To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function.
28863216	11	52	theme	LSC	1801:1803	arg1	phenotype					1805:1813	the LSC phenotype	1797:1813	the LSC phenotype	1797:1813	Finally, our findings suggest that HA has a major role in maintaining the LSC phenotype.
28863216	1	53	theme	Limbal	115:120	arg1	LSCs					145:148	LSCs	145:148	LSCs	145:148	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	1	53	theme	Limbal	115:120	arg1	cells					138:142	Purpose Limbal epithelial stem cells	107:142	Purpose Limbal epithelial stem cells (LSCs)	107:149	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	5	54	contain	have	841:844	arg2	HAS3					889:892	HAS3	889:892	HAS3	889:892	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	54	contain	have	841:844	arg2	HAS2					879:882	HAS2	879:882	HAS2	879:882	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	54	contain	have	841:844	arg2	HAS1					873:876	HAS1	873:876	HAS1	873:876	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	54	contain	have	841:844	arg2	types					866:870	the following three types	846:870	the following three types: HAS1, HAS2, and HAS3	846:892	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	54	contain	have	841:844	arg1	vertebrates					829:839	vertebrates	829:839	vertebrates	829:839	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	10	55	theme	epithelial	1702:1711	arg1	regeneration					1713:1724	compromised corneal epithelial regeneration	1682:1724	compromised corneal epithelial regeneration	1682:1724	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	1	56	theme	stem	133:136	arg1	LSCs					145:148	LSCs	145:148	LSCs	145:148	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	1	56	theme	stem	133:136	arg1	cells					138:142	Purpose Limbal epithelial stem cells	107:142	Purpose Limbal epithelial stem cells (LSCs)	107:149	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	4	57	theme	matrix	665:670	arg1	composition					632:642	the composition	628:642	the composition of the extracellular matrix	628:670	Our work investigates the composition of the extracellular matrix in the LSC niche and how it regulates LSC differentiation and function.
28863216	10	58	theme	compromised	1682:1692	arg1	regeneration					1713:1724	compromised corneal epithelial regeneration	1682:1724	compromised corneal epithelial regeneration	1682:1724	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	10	59	theme	corneal	1586:1592	arg1	epithelium					1594:1603	the corneal epithelium	1582:1603	the corneal epithelium	1582:1603	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	0	60	theme	Stem	80:83	arg1	Differentiation					90:104	Limbal Stem Cell Differentiation	73:104	Limbal Stem Cell Differentiation	73:104	Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation.
28863216	5	61	dep	types	866:870	arg1	HAS2					879:882	HAS2	879:882	HAS2	879:882	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	61	dep	types	866:870	arg1	HAS3					889:892	HAS3	889:892	HAS3	889:892	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	61	dep	types	866:870	arg1	HAS1					873:876	HAS1	873:876	HAS1	873:876	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	61	dep	types	866:870	arg1	types					866:870	the following three types	846:870	the following three types: HAS1, HAS2, and HAS3	846:892	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	11	62	contain	has	1765:1767	arg1	HA					1762:1763	HA	1762:1763	HA	1762:1763	Finally, our findings suggest that HA has a major role in maintaining the LSC phenotype.
28863216	11	62	contain	has	1765:1767	arg2	role					1777:1780	a major role	1769:1780	a major role	1769:1780	Finally, our findings suggest that HA has a major role in maintaining the LSC phenotype.
28863216	8	63	theme	wound	1232:1236	arg1	healing					1238:1244	wound healing	1232:1244	wound healing	1232:1244	HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, and TSG-6-/- mice have delayed wound healing and increased inflammation after injury.
28863216	11	64	theme	major	1771:1775	arg1	role					1777:1780	a major role	1769:1780	a major role	1769:1780	Finally, our findings suggest that HA has a major role in maintaining the LSC phenotype.
28863216	5	65	theme	Methods	744:750	arg1	Hyaluronan					752:761	Methods Hyaluronan	744:761	Methods Hyaluronan (HA)	744:766	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	5	65	theme	Methods	744:750	arg1	HA					764:765	HA	764:765	HA	764:765	Methods Hyaluronan (HA) is naturally synthesized by hyaluronan synthases (HASs), and vertebrates have the following three types: HAS1, HAS2, and HAS3.
28863216	9	66	theme	cell	1451:1454	arg1	specification					1456:1468	epithelial cell specification	1440:1468	epithelial cell specification	1440:1468	Interestingly, upon insult the HAS knock-out mice up-regulate HA throughout the cornea through a compensatory mechanism, and in turn this alters LSC and epithelial cell specification.
28863216	10	67	theme	HA	1526:1527	arg1	matrix					1529:1534	a specialized HA matrix	1512:1534	a specialized HA matrix that differs from that present in the rest of the corneal epithelium	1512:1603	Conclusions The LSC niche is composed of a specialized HA matrix that differs from that present in the rest of the corneal epithelium, and the disruption of this specific HA matrix within the LSC niche leads to compromised corneal epithelial regeneration.
28863216	6	68	dep	TSG-6-/--were	969:981	arg1	used					983:986	used	983:986	TSG-6-/--were used to determine the importance of the HA niche in LSC differentiation and specification	969:1071	Wild-type and HAS and TSG-6 knockout mice-HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, TSG-6-/--were used to determine the importance of the HA niche in LSC differentiation and specification.
28863216	0	69	theme	Limbal	40:45	arg1	Niche					57:61	the Limbal Stem Cell Niche	36:61	the Limbal Stem Cell Niche	36:61	Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation.
28863216	1	70	theme	corneal	258:264	arg1	epithelium					266:275	the corneal epithelium	254:275	the corneal epithelium	254:275	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
28863216	2	71	theme	poor	378:381	arg1	differentiation					383:397	poor differentiation	378:397	poor differentiation	378:397	LSCs have a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation.
28863216	0	72	theme	Cell	52:55	arg1	Niche					57:61	the Limbal Stem Cell Niche	36:61	the Limbal Stem Cell Niche	36:61	Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation.
28863216	2	73	with	capacity	295:302	arg1	potential					335:343	increased potential	325:343	increased potential for error-free proliferation and poor differentiation	325:397	LSCs have a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation.
28863216	6	74	theme	niche	1026:1030	arg1	importance					1005:1014	the importance	1001:1014	the importance of the HA niche in LSC differentiation and specification	1001:1071	Wild-type and HAS and TSG-6 knockout mice-HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, TSG-6-/--were used to determine the importance of the HA niche in LSC differentiation and specification.
28863216	2	75	theme	increased	325:333	arg1	potential					335:343	increased potential	325:343	increased potential for error-free proliferation and poor differentiation	325:397	LSCs have a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation.
28863216	3	76	theme	stem	481:484	arg1	niche					491:495	the limbal stem cell niche	470:495	the limbal stem cell niche	470:495	To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function.
28863216	2	77	contain	have	283:286	arg1	LSCs					278:281	LSCs	278:281	LSCs	278:281	LSCs have a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation.
28863216	2	77	contain	have	283:286	arg2	capacity					295:302	a high capacity	288:302	a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation	288:397	LSCs have a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation.
28863216	7	78	theme	extracellular	1147:1159	arg1	matrix					1161:1166	a HA rich extracellular matrix	1137:1166	a HA rich extracellular matrix	1137:1166	Results Our data demonstrate that the LSC niche is composed of a HA rich extracellular matrix.
28863216	4	79	dep	investigates	615:626	arg1	regulates					700:708	regulates	700:708	regulates LSC differentiation and function	700:741	Our work investigates the composition of the extracellular matrix in the LSC niche and how it regulates LSC differentiation and function.
28863216	3	80	theme	niche	491:495	arg1	composition					455:465	the composition	451:465	the composition of the limbal stem cell niche	451:495	To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function.
28863216	7	81	theme	HA	1139:1140	arg1	matrix					1161:1166	a HA rich extracellular matrix	1137:1166	a HA rich extracellular matrix	1137:1166	Results Our data demonstrate that the LSC niche is composed of a HA rich extracellular matrix.
28863216	3	82	contain	have	564:567	arg1	niche					554:558	the specific stem cell niche	531:558	the specific stem cell niche	531:558	To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function.
28863216	3	82	contain	have	564:567	arg2	function					596:603	function	596:603	function	596:603	To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function.
28863216	3	82	contain	have	564:567	arg2	differentiation					576:590	differentiation	576:590	differentiation	576:590	To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function.
28863216	6	83	from	importance	1005:1014	arg1	LSC					1035:1037	LSC	1035:1037	LSC	1035:1037	Wild-type and HAS and TSG-6 knockout mice-HAS1-/-;HAS3-/-, HAS2Δ/ΔCorEpi, TSG-6-/--were used to determine the importance of the HA niche in LSC differentiation and specification.
28863216	0	84	theme	Rich	11:14	arg1	Microenvironment					16:31	Hyaluronan Rich Microenvironment	0:31	Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche	0:61	Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation.
28863216	1	85	theme	corneal	212:218	arg1	limbus					220:225	the corneal limbus	208:225	the corneal limbus	208:225	Purpose Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium.
26518696	12	0	theme	pathogenic	1878:1887	arg1	LPS					1930:1932	pathogenic and intermediately pathogenic Leptospira LPS	1878:1932	pathogenic and intermediately pathogenic Leptospira LPS	1878:1932	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	11	1	theme	electron	1669:1676	arg1	examination					1690:1700	Negative staining electron microscopic examination	1651:1700	Negative staining electron microscopic examination of LPS	1651:1707	Negative staining electron microscopic examination of LPS showed different filamentous morphologies in L. interrogans serovar Copenhageni vs. L. licerasiae serovar Varillal.
26518696	7	2	theme	molecular	1123:1131	arg1	weight					1133:1138	a far higher molecular weight	1110:1138	a far higher molecular weight	1110:1138	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	8	3	contain	has	1272:1274	arg2	polysaccharide					1300:1313	an extended O-antigenic polysaccharide	1276:1313	an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal	1276:1381	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	8	3	contain	has	1272:1274	arg1	LPS					1268:1270	L. interrogans serovar Copenhageni LPS	1233:1270	L. interrogans serovar Copenhageni LPS	1233:1270	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	11	4	theme	different	1716:1724	arg1	morphologies					1738:1749	different filamentous morphologies	1716:1749	different filamentous morphologies	1716:1749	Negative staining electron microscopic examination of LPS showed different filamentous morphologies in L. interrogans serovar Copenhageni vs. L. licerasiae serovar Varillal.
26518696	11	5	dep	L.	1793:1794	arg1	serovar					1807:1813	L. interrogans serovar Copenhageni vs. L. licerasiae serovar	1754:1813	serovar	1807:1813	Negative staining electron microscopic examination of LPS showed different filamentous morphologies in L. interrogans serovar Copenhageni vs. L. licerasiae serovar Varillal.
26518696	12	6	theme	comparative	1842:1852	arg1	analysis					1866:1873	This comparative biochemical analysis	1837:1873	This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS	1837:1932	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	4	7	theme	VAR	709:711	arg1	species					747:753	an intermediately pathogenic species	718:753	an intermediately pathogenic species	718:753	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	4	7	theme	VAR	709:711	arg1	010					713:715	L. licerasiae serovar Varillal strain VAR 010	671:715	L. licerasiae serovar Varillal strain VAR 010	671:715	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	11	8	theme	LPS	1705:1707	arg1	examination					1690:1700	Negative staining electron microscopic examination	1651:1700	Negative staining electron microscopic examination of LPS	1651:1707	Negative staining electron microscopic examination of LPS showed different filamentous morphologies in L. interrogans serovar Copenhageni vs. L. licerasiae serovar Varillal.
26518696	7	9	theme	licerasiae	1171:1180	arg1	Varillal					1190:1197	L. licerasiae serovar Varillal	1168:1197	L. licerasiae serovar Varillal	1168:1197	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	1	10	theme	major	203:207	arg1	component					217:225	the major surface component	199:225	the major surface component of Gram-negative bacteria	199:251	BACKGROUND Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria.
26518696	7	11	theme	Copenhageni	1090:1100	arg1	LPS					1102:1104	L. interrogans serovar Copenhageni LPS	1067:1104	L. interrogans serovar Copenhageni LPS	1067:1104	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	8	12	theme	Chemical	1200:1207	arg1	composition					1209:1219	Chemical composition	1200:1219	Chemical composition	1200:1219	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	4	13	theme	licerasiae	674:683	arg1	species					747:753	an intermediately pathogenic species	718:753	an intermediately pathogenic species	718:753	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	4	13	theme	licerasiae	674:683	arg1	010					713:715	L. licerasiae serovar Varillal strain VAR 010	671:715	L. licerasiae serovar Varillal strain VAR 010	671:715	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	7	14	theme	interrogans	1070:1080	arg1	LPS					1102:1104	L. interrogans serovar Copenhageni LPS	1067:1104	L. interrogans serovar Copenhageni LPS	1067:1104	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	1	15	theme	Gram-negative	230:242	arg1	bacteria					244:251	Gram-negative bacteria	230:251	Gram-negative bacteria	230:251	BACKGROUND Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria.
26518696	12	16	theme	pathogenicity	2065:2077	arg1	mechanisms					2034:2043	the mechanisms	2030:2043	the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis	2030:2118	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	4	17	theme	Varillal	693:700	arg1	species					747:753	an intermediately pathogenic species	718:753	an intermediately pathogenic species	718:753	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	4	17	theme	Varillal	693:700	arg1	010					713:715	L. licerasiae serovar Varillal strain VAR 010	671:715	L. licerasiae serovar Varillal strain VAR 010	671:715	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	8	18	theme	L.	1233:1234	arg1	LPS					1268:1270	L. interrogans serovar Copenhageni LPS	1233:1270	L. interrogans serovar Copenhageni LPS	1233:1270	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	6	19	theme	high	969:972	arg1	GlcNAc					984:989	GlcNAc	984:989	GlcNAc (12.3%)	984:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	19	theme	high	969:972	arg1	%					996:996	12.3%	992:996	12.3%	992:996	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	19	theme	high	969:972	arg1	amount					974:979	a high amount	967:979	a high amount of GlcNAc (12.3%)	967:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	19	theme	high	969:972	arg1	HexNAc					1035:1040	dideoxy HexNAc	1027:1040	dideoxy HexNAc	1027:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	0	20	theme	pathogenic	77:86	arg1	species					99:105	pathogenic and intermediately pathogenic Leptospira species	47:105	pathogenic and intermediately pathogenic Leptospira species	47:105	Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species.
26518696	2	21	theme	human-pathogenic	279:294	arg1	spirochetes					296:306	other human-pathogenic spirochetes	273:306	other human-pathogenic spirochetes	273:306	Leptospira, unlike other human-pathogenic spirochetes, produce LPS, which is fundamental to the taxonomy of the genus, involved in host-adaption and also the target of diagnostic antibodies.
26518696	0	22	theme	species	99:105	arg1	lipopolysaccharides					24:42	lipopolysaccharides	24:42	lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species	24:105	Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species.
26518696	4	23	dep	RESULTS	570:576	arg1	studied					761:767	studied	761:767	were studied	756:767	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	1	24	dep	complex	149:155	arg1	amphipathic					158:168	amphipathic	158:168	amphipathic	158:168	BACKGROUND Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria.
26518696	12	25	dep	CONCLUSIONS	1825:1835	arg1	reveals					1934:1940	reveals	1934:1940	reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis	1934:2118	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	4	26	from	strain	630:635	arg1	LPS					578:580	LPS	578:580	LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species,	578:754	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	12	27	theme	future	2001:2006	arg1	work					2008:2011	future work	2001:2011	future work	2001:2011	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	10	28	theme	Fatty	1486:1490	arg1	analysis					1497:1504	Fatty acid analysis	1486:1504	Fatty acid analysis by gas chromatography-mass spectrometry (GC-MS)	1486:1552	Fatty acid analysis by gas chromatography-mass spectrometry (GC-MS) showed the presence of hydroxypalmitate (3-OH-C16:0) only in L. interrogans serovar Copenhageni.
26518696	0	29	theme	Comparative	0:10	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species	0:105	Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species.
26518696	4	30	theme	Leptospira	587:596	arg1	strain					630:635	Leptospira interrogans serovar Copenhageni strain L1-130	587:642	Leptospira interrogans serovar Copenhageni strain L1-130	587:642	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	12	31	theme	lipid	1969:1973	arg1	differences					1975:1985	important carbohydrate and lipid differences	1942:1985	differences	1975:1985	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	6	32	theme	serovar	924:930	arg1	Varillal					932:939	L. licerasiae serovar Varillal	910:939	L. licerasiae serovar Varillal	910:939	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	4	33	theme	serovar	610:616	arg1	strain					630:635	Leptospira interrogans serovar Copenhageni strain L1-130	587:642	Leptospira interrogans serovar Copenhageni strain L1-130	587:642	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	10	34	theme	gas	1509:1511	arg1	GC-MS					1547:1551	GC-MS	1547:1551	GC-MS	1547:1551	Fatty acid analysis by gas chromatography-mass spectrometry (GC-MS) showed the presence of hydroxypalmitate (3-OH-C16:0) only in L. interrogans serovar Copenhageni.
26518696	10	34	theme	gas	1509:1511	arg1	spectrometry					1533:1544	gas chromatography-mass spectrometry	1509:1544	gas chromatography-mass spectrometry (GC-MS)	1509:1552	Fatty acid analysis by gas chromatography-mass spectrometry (GC-MS) showed the presence of hydroxypalmitate (3-OH-C16:0) only in L. interrogans serovar Copenhageni.
26518696	4	35	theme	serovar	685:691	arg1	species					747:753	an intermediately pathogenic species	718:753	an intermediately pathogenic species	718:753	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	4	35	theme	serovar	685:691	arg1	010					713:715	L. licerasiae serovar Varillal strain VAR 010	671:715	L. licerasiae serovar Varillal strain VAR 010	671:715	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	2	36	theme	antibodies	433:442	arg1	target					412:417	the target	408:417	the target of diagnostic antibodies	408:442	Leptospira, unlike other human-pathogenic spirochetes, produce LPS, which is fundamental to the taxonomy of the genus, involved in host-adaption and also the target of diagnostic antibodies.
26518696	8	37	theme	serovar	1248:1254	arg1	LPS					1268:1270	L. interrogans serovar Copenhageni LPS	1233:1270	L. interrogans serovar Copenhageni LPS	1233:1270	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	6	38	theme	L.	910:911	arg1	Varillal					932:939	L. licerasiae serovar Varillal	910:939	L. licerasiae serovar Varillal	910:939	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	12	39	from	mechanisms	2034:2043	arg1	leptospirosis					2106:2118	leptospirosis	2106:2118	leptospirosis	2106:2118	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	12	40	theme	pathogenic	1908:1917	arg1	LPS					1930:1932	pathogenic and intermediately pathogenic Leptospira LPS	1878:1932	pathogenic and intermediately pathogenic Leptospira LPS	1878:1932	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	6	41	theme	dideoxy	1027:1033	arg1	HexNAc					1035:1040	dideoxy HexNAc	1027:1040	dideoxy HexNAc	1027:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	11	42	dep	different	1716:1724	arg1	filamentous					1726:1736	filamentous	1726:1736	filamentous	1726:1736	Negative staining electron microscopic examination of LPS showed different filamentous morphologies in L. interrogans serovar Copenhageni vs. L. licerasiae serovar Varillal.
26518696	12	43	theme	LPS	1930:1932	arg1	analysis					1866:1873	This comparative biochemical analysis	1837:1873	This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS	1837:1932	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	3	44	theme	LPS	501:503	arg1	composition					505:515	Leptospira LPS composition	490:515	Leptospira LPS composition	490:515	Despite its significance, little is known of Leptospira LPS composition and carbohydrate structure among different serovars.
26518696	4	45	theme	pathogenic	647:656	arg1	species					658:664	a pathogenic species	645:664	a pathogenic species	645:664	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	10	46	theme	hydroxypalmitate	1577:1592	arg1	presence					1565:1572	the presence	1561:1572	the presence of hydroxypalmitate (3-OH-C16:0)	1561:1605	Fatty acid analysis by gas chromatography-mass spectrometry (GC-MS) showed the presence of hydroxypalmitate (3-OH-C16:0) only in L. interrogans serovar Copenhageni.
26518696	11	47	theme	staining	1660:1667	arg1	examination					1690:1700	Negative staining electron microscopic examination	1651:1700	Negative staining electron microscopic examination of LPS	1651:1707	Negative staining electron microscopic examination of LPS showed different filamentous morphologies in L. interrogans serovar Copenhageni vs. L. licerasiae serovar Varillal.
26518696	3	48	theme	different	550:558	arg1	serovars					560:567	different serovars	550:567	different serovars	550:567	Despite its significance, little is known of Leptospira LPS composition and carbohydrate structure among different serovars.
26518696	4	49	theme	strain	702:707	arg1	species					747:753	an intermediately pathogenic species	718:753	an intermediately pathogenic species	718:753	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	4	49	theme	strain	702:707	arg1	010					713:715	L. licerasiae serovar Varillal strain VAR 010	671:715	L. licerasiae serovar Varillal strain VAR 010	671:715	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	7	50	theme	higher	1116:1121	arg1	weight					1133:1138	a far higher molecular weight	1110:1138	a far higher molecular weight	1110:1138	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	11	51	theme	microscopic	1678:1688	arg1	examination					1690:1700	Negative staining electron microscopic examination	1651:1700	Negative staining electron microscopic examination of LPS	1651:1707	Negative staining electron microscopic examination of LPS showed different filamentous morphologies in L. interrogans serovar Copenhageni vs. L. licerasiae serovar Varillal.
26518696	12	52	theme	biochemical	1854:1864	arg1	analysis					1866:1873	This comparative biochemical analysis	1837:1873	This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS	1837:1932	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	10	53	dep	L.	1615:1616	arg1	serovar					1630:1636	L. interrogans serovar	1615:1636	L. interrogans serovar Copenhageni	1615:1648	Fatty acid analysis by gas chromatography-mass spectrometry (GC-MS) showed the presence of hydroxypalmitate (3-OH-C16:0) only in L. interrogans serovar Copenhageni.
26518696	4	54	from	010	713:715	arg1	LPS					578:580	LPS	578:580	LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species,	578:754	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	6	55	dep	L.	840:841	arg1	serovar					855:861	L. interrogans serovar	840:861	L. interrogans serovar Copenhageni	840:873	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	8	56	theme	interrogans	1236:1246	arg1	LPS					1268:1270	L. interrogans serovar Copenhageni LPS	1233:1270	L. interrogans serovar Copenhageni LPS	1233:1270	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	7	57	theme	serovar	1082:1088	arg1	LPS					1102:1104	L. interrogans serovar Copenhageni LPS	1067:1104	L. interrogans serovar Copenhageni LPS	1067:1104	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	1	58	theme	surface	209:215	arg1	component					217:225	the major surface component	199:225	the major surface component of Gram-negative bacteria	199:251	BACKGROUND Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria.
26518696	12	59	theme	development	2091:2101	arg1	mechanisms					2034:2043	the mechanisms	2030:2043	the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis	2030:2118	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	8	60	theme	Copenhageni	1256:1266	arg1	LPS					1268:1270	L. interrogans serovar Copenhageni LPS	1233:1270	L. interrogans serovar Copenhageni LPS	1233:1270	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	7	61	theme	L.	1067:1068	arg1	LPS					1102:1104	L. interrogans serovar Copenhageni LPS	1067:1104	L. interrogans serovar Copenhageni LPS	1067:1104	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	6	62	theme	GlcNAc	984:989	arg1	%					963:963	2.7%	960:963	2.7%	960:963	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	62	theme	GlcNAc	984:989	arg1	HexNAc					1035:1040	dideoxy HexNAc	1027:1040	dideoxy HexNAc	1027:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	62	theme	GlcNAc	984:989	arg1	GlcNAc					984:989	GlcNAc	984:989	GlcNAc (12.3%)	984:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	62	theme	GlcNAc	984:989	arg1	fucose					952:957	fucose	952:957	fucose (2.7%)	952:964	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	62	theme	GlcNAc	984:989	arg1	types					1018:1022	two different types	1004:1022	two different types of dideoxy HexNAc	1004:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	62	theme	GlcNAc	984:989	arg1	%					996:996	12.3%	992:996	12.3%	992:996	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	62	theme	GlcNAc	984:989	arg1	amount					974:979	a high amount	967:979	a high amount of GlcNAc (12.3%)	967:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	8	63	theme	present	1341:1347	arg1	sugars					1329:1334	sugars	1329:1334	sugars	1329:1334	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	4	64	theme	L.	671:672	arg1	species					747:753	an intermediately pathogenic species	718:753	an intermediately pathogenic species	718:753	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	4	64	theme	L.	671:672	arg1	010					713:715	L. licerasiae serovar Varillal strain VAR 010	671:715	L. licerasiae serovar Varillal strain VAR 010	671:715	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	2	65	theme	genus	366:370	arg1	taxonomy					350:357	the taxonomy	346:357	the taxonomy	346:357	Leptospira, unlike other human-pathogenic spirochetes, produce LPS, which is fundamental to the taxonomy of the genus, involved in host-adaption and also the target of diagnostic antibodies.
26518696	2	66	theme	other	273:277	arg1	spirochetes					296:306	other human-pathogenic spirochetes	273:306	other human-pathogenic spirochetes	273:306	Leptospira, unlike other human-pathogenic spirochetes, produce LPS, which is fundamental to the taxonomy of the genus, involved in host-adaption and also the target of diagnostic antibodies.
26518696	1	67	theme	bacteria	244:251	arg1	component					217:225	the major surface component	199:225	the major surface component of Gram-negative bacteria	199:251	BACKGROUND Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria.
26518696	8	68	theme	O-antigenic	1288:1298	arg1	polysaccharide					1300:1313	an extended O-antigenic polysaccharide	1276:1313	an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal	1276:1381	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	8	69	theme	extended	1279:1286	arg1	polysaccharide					1300:1313	an extended O-antigenic polysaccharide	1276:1313	an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal	1276:1381	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	5	70	dep	aqueous	788:794	arg1	phases					807:812	phases	807:812	phases	807:812	LPS prepared from aqueous and phenol phases were analyzed separately.
26518696	6	71	located	found	901:905	arg2	types					1018:1022	two different types	1004:1022	two different types of dideoxy HexNAc	1004:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	71	located	found	901:905	arg2	fucose					952:957	fucose	952:957	fucose (2.7%)	952:964	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	71	located	found	901:905	arg2	sugars					890:895	additional sugars	879:895	additional sugars	879:895	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	71	located	found	901:905	arg1	Varillal					932:939	L. licerasiae serovar Varillal	910:939	L. licerasiae serovar Varillal	910:939	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	71	located	found	901:905	arg2	amount					974:979	a high amount	967:979	a high amount of GlcNAc (12.3%)	967:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	71	located	found	901:905	arg2	GlcNAc					984:989	GlcNAc	984:989	GlcNAc (12.3%)	984:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	71	located	found	901:905	arg2	HexNAc					1035:1040	dideoxy HexNAc	1027:1040	dideoxy HexNAc	1027:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	8	72	attach	present	1341:1347	arg1	Varillal					1374:1381	L. licerasiae serovar Varillal	1352:1381	L. licerasiae serovar Varillal	1352:1381	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	8	72	attach	present	1341:1347	arg2	sugars					1329:1334	sugars	1329:1334	sugars	1329:1334	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	1	73	theme	complex	149:155	arg1	biomolecules					170:181	complex, amphipathic biomolecules	149:181	complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria	149:251	BACKGROUND Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria.
26518696	1	73	theme	complex	149:155	arg1	Lipopolysaccharides					119:137	BACKGROUND Lipopolysaccharides	108:137	BACKGROUND Lipopolysaccharides (LPS)	108:143	BACKGROUND Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria.
26518696	8	74	theme	licerasiae	1355:1364	arg1	Varillal					1374:1381	L. licerasiae serovar Varillal	1352:1381	L. licerasiae serovar Varillal	1352:1381	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	7	75	contain	had	1106:1108	arg1	LPS					1102:1104	L. interrogans serovar Copenhageni LPS	1067:1104	L. interrogans serovar Copenhageni LPS	1067:1104	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	7	75	contain	had	1106:1108	arg2	weight					1133:1138	a far higher molecular weight	1110:1138	a far higher molecular weight	1110:1138	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	7	75	contain	had	1106:1108	arg2	complexity					1144:1153	complexity	1144:1153	complexity	1144:1153	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	0	76	theme	Leptospira	88:97	arg1	species					99:105	pathogenic and intermediately pathogenic Leptospira species	47:105	pathogenic and intermediately pathogenic Leptospira species	47:105	Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species.
26518696	6	77	theme	different	1008:1016	arg1	types					1018:1022	two different types	1004:1022	two different types of dideoxy HexNAc	1004:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	4	78	from	species	658:664	arg1	LPS					578:580	LPS	578:580	LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species,	578:754	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	6	79	contain	has	875:877	arg2	types					1018:1022	two different types	1004:1022	two different types of dideoxy HexNAc	1004:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	79	contain	has	875:877	arg2	fucose					952:957	fucose	952:957	fucose (2.7%)	952:964	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	79	contain	has	875:877	arg2	sugars					890:895	additional sugars	879:895	additional sugars	879:895	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	79	contain	has	875:877	arg2	amount					974:979	a high amount	967:979	a high amount of GlcNAc (12.3%)	967:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	79	contain	has	875:877	arg2	GlcNAc					984:989	GlcNAc	984:989	GlcNAc (12.3%)	984:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	79	contain	has	875:877	arg1	Copenhageni					863:873	Copenhageni	863:873	Copenhageni	863:873	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	79	contain	has	875:877	arg2	HexNAc					1035:1040	dideoxy HexNAc	1027:1040	dideoxy HexNAc	1027:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	4	80	theme	pathogenic	736:745	arg1	species					747:753	an intermediately pathogenic species	718:753	an intermediately pathogenic species	718:753	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	4	80	theme	pathogenic	736:745	arg1	010					713:715	L. licerasiae serovar Varillal strain VAR 010	671:715	L. licerasiae serovar Varillal strain VAR 010	671:715	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	12	81	theme	host-adaptation	2048:2062	arg1	mechanisms					2034:2043	the mechanisms	2030:2043	the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis	2030:2118	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	8	82	theme	L.	1352:1353	arg1	Varillal					1374:1381	L. licerasiae serovar Varillal	1352:1381	L. licerasiae serovar Varillal	1352:1381	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	7	83	theme	serovar	1182:1188	arg1	Varillal					1190:1197	L. licerasiae serovar Varillal	1168:1197	L. licerasiae serovar Varillal	1168:1197	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	10	84	theme	acid	1492:1495	arg1	analysis					1497:1504	Fatty acid analysis	1486:1504	Fatty acid analysis by gas chromatography-mass spectrometry (GC-MS)	1486:1552	Fatty acid analysis by gas chromatography-mass spectrometry (GC-MS) showed the presence of hydroxypalmitate (3-OH-C16:0) only in L. interrogans serovar Copenhageni.
26518696	8	85	theme	serovar	1366:1372	arg1	Varillal					1374:1381	L. licerasiae serovar Varillal	1352:1381	L. licerasiae serovar Varillal	1352:1381	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	7	86	theme	L.	1168:1169	arg1	Varillal					1190:1197	L. licerasiae serovar Varillal	1168:1197	L. licerasiae serovar Varillal	1168:1197	SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal.
26518696	8	87	from	present	1341:1347	arg1	Varillal					1374:1381	L. licerasiae serovar Varillal	1352:1381	L. licerasiae serovar Varillal	1352:1381	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	1	88	theme	BACKGROUND	108:117	arg1	Lipopolysaccharides					119:137	BACKGROUND Lipopolysaccharides	108:137	BACKGROUND Lipopolysaccharides (LPS)	108:143	BACKGROUND Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria.
26518696	1	88	theme	BACKGROUND	108:117	arg1	biomolecules					170:181	complex, amphipathic biomolecules	149:181	complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria	149:251	BACKGROUND Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria.
26518696	1	88	theme	BACKGROUND	108:117	arg1	LPS					140:142	LPS	140:142	LPS	140:142	BACKGROUND Lipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria.
26518696	4	89	theme	interrogans	598:608	arg1	strain					630:635	Leptospira interrogans serovar Copenhageni strain L1-130	587:642	Leptospira interrogans serovar Copenhageni strain L1-130	587:642	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	9	90	located	detected	1456:1463	arg1	species					1477:1483	both the species	1468:1483	both the species	1468:1483	Arabinose, xylose, mannose, galactose and L-glycero-D-mannoheptose were detected in both the species.
26518696	9	90	located	detected	1456:1463	arg2	mannose					1403:1409	mannose	1403:1409	mannose	1403:1409	Arabinose, xylose, mannose, galactose and L-glycero-D-mannoheptose were detected in both the species.
26518696	9	90	located	detected	1456:1463	arg2	Arabinose					1384:1392	Arabinose	1384:1392	Arabinose	1384:1392	Arabinose, xylose, mannose, galactose and L-glycero-D-mannoheptose were detected in both the species.
26518696	9	90	located	detected	1456:1463	arg2	galactose					1412:1420	galactose	1412:1420	galactose	1412:1420	Arabinose, xylose, mannose, galactose and L-glycero-D-mannoheptose were detected in both the species.
26518696	9	90	located	detected	1456:1463	arg2	xylose					1395:1400	xylose	1395:1400	xylose	1395:1400	Arabinose, xylose, mannose, galactose and L-glycero-D-mannoheptose were detected in both the species.
26518696	9	90	located	detected	1456:1463	arg2	L-glycero-D-mannoheptose					1426:1449	L-glycero-D-mannoheptose	1426:1449	L-glycero-D-mannoheptose	1426:1449	Arabinose, xylose, mannose, galactose and L-glycero-D-mannoheptose were detected in both the species.
26518696	0	91	theme	lipopolysaccharides	24:42	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species	0:105	Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species.
26518696	4	92	theme	Copenhageni	618:628	arg1	strain					630:635	Leptospira interrogans serovar Copenhageni strain L1-130	587:642	Leptospira interrogans serovar Copenhageni strain L1-130	587:642	RESULTS LPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied.
26518696	10	93	theme	chromatography-mass	1513:1531	arg1	GC-MS					1547:1551	GC-MS	1547:1551	GC-MS	1547:1551	Fatty acid analysis by gas chromatography-mass spectrometry (GC-MS) showed the presence of hydroxypalmitate (3-OH-C16:0) only in L. interrogans serovar Copenhageni.
26518696	10	93	theme	chromatography-mass	1513:1531	arg1	spectrometry					1533:1544	gas chromatography-mass spectrometry	1509:1544	gas chromatography-mass spectrometry (GC-MS)	1509:1552	Fatty acid analysis by gas chromatography-mass spectrometry (GC-MS) showed the presence of hydroxypalmitate (3-OH-C16:0) only in L. interrogans serovar Copenhageni.
26518696	3	94	theme	Leptospira	490:499	arg1	composition					505:515	Leptospira LPS composition	490:515	Leptospira LPS composition	490:515	Despite its significance, little is known of Leptospira LPS composition and carbohydrate structure among different serovars.
26518696	0	95	theme	pathogenic	47:56	arg1	species					99:105	pathogenic and intermediately pathogenic Leptospira species	47:105	pathogenic and intermediately pathogenic Leptospira species	47:105	Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species.
26518696	6	96	theme	licerasiae	913:922	arg1	Varillal					932:939	L. licerasiae serovar Varillal	910:939	L. licerasiae serovar Varillal	910:939	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	97	theme	HexNAc	1035:1040	arg1	%					963:963	2.7%	960:963	2.7%	960:963	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	97	theme	HexNAc	1035:1040	arg1	HexNAc					1035:1040	dideoxy HexNAc	1027:1040	dideoxy HexNAc	1027:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	97	theme	HexNAc	1035:1040	arg1	GlcNAc					984:989	GlcNAc	984:989	GlcNAc (12.3%)	984:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	97	theme	HexNAc	1035:1040	arg1	fucose					952:957	fucose	952:957	fucose (2.7%)	952:964	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	97	theme	HexNAc	1035:1040	arg1	types					1018:1022	two different types	1004:1022	two different types of dideoxy HexNAc	1004:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	97	theme	HexNAc	1035:1040	arg1	%					996:996	12.3%	992:996	12.3%	992:996	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	97	theme	HexNAc	1035:1040	arg1	amount					974:979	a high amount	967:979	a high amount of GlcNAc (12.3%)	967:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	2	98	theme	diagnostic	422:431	arg1	antibodies					433:442	diagnostic antibodies	422:442	diagnostic antibodies	422:442	Leptospira, unlike other human-pathogenic spirochetes, produce LPS, which is fundamental to the taxonomy of the genus, involved in host-adaption and also the target of diagnostic antibodies.
26518696	12	99	theme	important	1942:1950	arg1	carbohydrate					1952:1963	important carbohydrate and lipid differences	1942:1985	carbohydrate	1952:1963	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	3	100	theme	carbohydrate	521:532	arg1	structure					534:542	carbohydrate structure	521:542	carbohydrate structure	521:542	Despite its significance, little is known of Leptospira LPS composition and carbohydrate structure among different serovars.
26518696	8	101	from	Varillal	1374:1381	arg1	present					1341:1347	present	1341:1347	present	1341:1347	Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal.
26518696	12	102	theme	Leptospira	1919:1928	arg1	LPS					1930:1932	pathogenic and intermediately pathogenic Leptospira LPS	1878:1932	pathogenic and intermediately pathogenic Leptospira LPS	1878:1932	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26518696	11	103	theme	Negative	1651:1658	arg1	examination					1690:1700	Negative staining electron microscopic examination	1651:1700	Negative staining electron microscopic examination of LPS	1651:1707	Negative staining electron microscopic examination of LPS showed different filamentous morphologies in L. interrogans serovar Copenhageni vs. L. licerasiae serovar Varillal.
26518696	6	104	theme	additional	879:888	arg1	sugars					890:895	additional sugars	879:895	additional sugars	879:895	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	104	theme	additional	879:888	arg1	GlcNAc					984:989	GlcNAc	984:989	GlcNAc (12.3%)	984:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	104	theme	additional	879:888	arg1	fucose					952:957	fucose	952:957	fucose (2.7%)	952:964	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	104	theme	additional	879:888	arg1	types					1018:1022	two different types	1004:1022	two different types of dideoxy HexNAc	1004:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	104	theme	additional	879:888	arg1	amount					974:979	a high amount	967:979	a high amount of GlcNAc (12.3%)	967:997	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	6	104	theme	additional	879:888	arg1	HexNAc					1035:1040	dideoxy HexNAc	1027:1040	dideoxy HexNAc	1027:1040	L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc.
26518696	12	105	theme	vaccine	2083:2089	arg1	development					2091:2101	vaccine development	2083:2101	vaccine development	2083:2101	CONCLUSIONS This comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
26876857	1	0	theme	pore	221:224	arg1	size					226:229	controlled pore size	210:229	controlled pore size	210:229	Chitosan nanofibers (average diameter of 75nm) were electrospun on polyester (PET) scrim to form composite nanofiber membranes with controlled pore size.
26876857	0	1	theme	nanofiber	57:65	arg1	membranes					67:75	electrospun chitosan nanofiber membranes	36:75	electrospun chitosan nanofiber membranes	36:75	Chemical filtration of Cr (VI) with electrospun chitosan nanofiber membranes.
26876857	6	2	theme	BDST	1171:1174	arg1	model					1177:1181	bed depth service time (BDST) model	1147:1181	bed depth service time (BDST) model	1147:1181	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	1	3	from	electrospun	130:140	arg1	scrim					161:165	polyester (PET) scrim	145:165	polyester (PET) scrim	145:165	Chitosan nanofibers (average diameter of 75nm) were electrospun on polyester (PET) scrim to form composite nanofiber membranes with controlled pore size.
26876857	2	4	theme	Cr	309:310	arg1	filtration					295:304	chemical filtration	286:304	chemical filtration of Cr (VI) of 1-5mg/L	286:326	The membranes were then stacked as a membrane bed for chemical filtration of Cr (VI) of 1-5mg/L.
26876857	6	5	theme	1g/m	1127:1130	arg1	density					1116:1122	nanofiber deposition density	1095:1122	nanofiber deposition density of 1g/m(2)	1095:1133	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	6	6	theme	stacked	1072:1078	arg1	membranes					1080:1088	stacked membranes	1072:1088	stacked membranes with nanofiber deposition density of 1g/m(2)	1072:1133	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	6	7	theme	nanofiber	1095:1103	arg1	density					1116:1122	nanofiber deposition density	1095:1122	nanofiber deposition density of 1g/m(2)	1095:1133	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	6	8	theme	time	1165:1168	arg1	model					1177:1181	bed depth service time (BDST) model	1147:1181	bed depth service time (BDST) model	1147:1181	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	5	9	from	breakthrough	762:773	arg1	16.5mg-chromium/g-chitosan					791:816	16.5mg-chromium/g-chitosan	791:816	16.5mg-chromium/g-chitosan	791:816	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	9	from	breakthrough	762:773	arg1	higher					819:824	higher	819:824	higher	819:824	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	9	from	breakthrough	762:773	arg1	capacity					721:728	The maximum bed loading capacity	697:728	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough	697:773	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	2	10	dep	Cr	309:310	arg1	VI					313:314	VI	313:314	VI	313:314	The membranes were then stacked as a membrane bed for chemical filtration of Cr (VI) of 1-5mg/L.
26876857	3	11	dep	performance	333:343	arg1	efficiency					437:446	efficiency	437:446	efficiency	437:446	The performance of the bed with respect to loading capacity at breakthrough, bed saturation and utilization efficiency were carefully investigated.
26876857	6	12	theme	bed	993:995	arg1	layers					1062:1067	three layers	1056:1067	three layers of stacked membranes with nanofiber deposition density of 1g/m(2)	1056:1133	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	6	12	theme	bed	993:995	arg1	length					997:1002	The minimum bed length	981:1002	The minimum bed length required to avoid breakthrough	981:1033	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	5	13	theme	11.0mg-chromium/g-chitosan	865:890	arg1	capacity					853:860	the static adsorption capacity	831:860	the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats	831:911	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	1	14	theme	PET	156:158	arg1	scrim					161:165	polyester (PET) scrim	145:165	polyester (PET) scrim	145:165	Chitosan nanofibers (average diameter of 75nm) were electrospun on polyester (PET) scrim to form composite nanofiber membranes with controlled pore size.
26876857	1	15	theme	polyester	145:153	arg1	scrim					161:165	polyester (PET) scrim	145:165	polyester (PET) scrim	145:165	Chitosan nanofibers (average diameter of 75nm) were electrospun on polyester (PET) scrim to form composite nanofiber membranes with controlled pore size.
26876857	6	16	theme	depth	1151:1155	arg1	model					1177:1181	bed depth service time (BDST) model	1147:1181	bed depth service time (BDST) model	1147:1181	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	5	17	theme	bed	709:711	arg1	16.5mg-chromium/g-chitosan					791:816	16.5mg-chromium/g-chitosan	791:816	16.5mg-chromium/g-chitosan	791:816	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	17	theme	bed	709:711	arg1	higher					819:824	higher	819:824	higher	819:824	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	17	theme	bed	709:711	arg1	capacity					721:728	The maximum bed loading capacity	697:728	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough	697:773	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	3	18	theme	loading	372:378	arg1	capacity					380:387	loading capacity	372:387	loading capacity at breakthrough	372:403	The performance of the bed with respect to loading capacity at breakthrough, bed saturation and utilization efficiency were carefully investigated.
26876857	5	19	theme	maximum	701:707	arg1	16.5mg-chromium/g-chitosan					791:816	16.5mg-chromium/g-chitosan	791:816	16.5mg-chromium/g-chitosan	791:816	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	19	theme	maximum	701:707	arg1	higher					819:824	higher	819:824	higher	819:824	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	19	theme	maximum	701:707	arg1	capacity					721:728	The maximum bed loading capacity	697:728	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough	697:773	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	6	20	theme	bed	1147:1149	arg1	model					1177:1181	bed depth service time (BDST) model	1147:1181	bed depth service time (BDST) model	1147:1181	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	6	21	with	membranes	1080:1088	arg1	density					1116:1122	nanofiber deposition density	1095:1122	nanofiber deposition density of 1g/m(2)	1095:1133	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	4	22	theme	feed	654:657	arg1	Cr					659:660	feed Cr (VI) concentration and bed length	654:694	feed Cr (VI) concentration and bed length	654:694	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	2	23	theme	1-5mg/L	320:326	arg1	Cr					309:310	Cr	309:310	Cr (VI) of 1-5mg/L	309:326	The membranes were then stacked as a membrane bed for chemical filtration of Cr (VI) of 1-5mg/L.
26876857	0	24	theme	Chemical	0:7	arg1	filtration					9:18	Chemical filtration	0:18	Chemical filtration of Cr (VI) with electrospun chitosan nanofiber membranes.	0:76	Chemical filtration of Cr (VI) with electrospun chitosan nanofiber membranes.
26876857	6	25	theme	service	1157:1163	arg1	model					1177:1181	bed depth service time (BDST) model	1147:1181	bed depth service time (BDST) model	1147:1181	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	2	26	theme	membrane	269:276	arg1	membranes					236:244	The membranes	232:244	The membranes	232:244	The membranes were then stacked as a membrane bed for chemical filtration of Cr (VI) of 1-5mg/L.
26876857	2	26	theme	membrane	269:276	arg1	bed					278:280	a membrane bed	267:280	a membrane bed for chemical filtration of Cr (VI) of 1-5mg/L	267:326	The membranes were then stacked as a membrane bed for chemical filtration of Cr (VI) of 1-5mg/L.
26876857	0	27	theme	Cr	23:24	arg1	filtration					9:18	Chemical filtration	0:18	Chemical filtration of Cr (VI) with electrospun chitosan nanofiber membranes.	0:76	Chemical filtration of Cr (VI) with electrospun chitosan nanofiber membranes.
26876857	6	28	theme	minimum	985:991	arg1	layers					1062:1067	three layers	1056:1067	three layers of stacked membranes with nanofiber deposition density of 1g/m(2)	1056:1133	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	6	28	theme	minimum	985:991	arg1	length					997:1002	The minimum bed length	981:1002	The minimum bed length required to avoid breakthrough	981:1033	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	5	29	theme	static	835:840	arg1	capacity					853:860	the static adsorption capacity	831:860	the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats	831:911	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	4	30	theme	flow	563:566	arg1	distribution					568:579	flow distribution	563:579	flow distribution	563:579	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	1	31	theme	Chitosan	78:85	arg1	nanofibers					87:96	Chitosan nanofibers	78:96	Chitosan nanofibers (average diameter of 75nm)	78:123	Chitosan nanofibers (average diameter of 75nm) were electrospun on polyester (PET) scrim to form composite nanofiber membranes with controlled pore size.
26876857	3	32	from	breakthrough	392:403	arg1	capacity					380:387	loading capacity	372:387	loading capacity at breakthrough	372:403	The performance of the bed with respect to loading capacity at breakthrough, bed saturation and utilization efficiency were carefully investigated.
26876857	5	33	theme	adsorption	842:851	arg1	capacity					853:860	the static adsorption capacity	831:860	the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats	831:911	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	34	theme	1mg/L	734:738	arg1	filtration					748:757	1mg/L Cr (VI) filtration	734:757	1mg/L Cr (VI) filtration	734:757	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	35	dep	filtration	748:757	arg1	VI					744:745	VI	744:745	VI	744:745	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	36	theme	Cr	740:741	arg1	filtration					748:757	1mg/L Cr (VI) filtration	734:757	1mg/L Cr (VI) filtration	734:757	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	3	37	theme	bed	352:354	arg1	utilization					425:435	utilization	425:435	utilization	425:435	The performance of the bed with respect to loading capacity at breakthrough, bed saturation and utilization efficiency were carefully investigated.
26876857	3	37	theme	bed	352:354	arg1	saturation					410:419	bed saturation	406:419	bed saturation	406:419	The performance of the bed with respect to loading capacity at breakthrough, bed saturation and utilization efficiency were carefully investigated.
26876857	3	37	theme	bed	352:354	arg1	performance					333:343	The performance	329:343	The performance of the bed with respect to loading capacity at breakthrough	329:403	The performance of the bed with respect to loading capacity at breakthrough, bed saturation and utilization efficiency were carefully investigated.
26876857	5	38	theme	loading	713:719	arg1	16.5mg-chromium/g-chitosan					791:816	16.5mg-chromium/g-chitosan	791:816	16.5mg-chromium/g-chitosan	791:816	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	38	theme	loading	713:719	arg1	higher					819:824	higher	819:824	higher	819:824	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	38	theme	loading	713:719	arg1	capacity					721:728	The maximum bed loading capacity	697:728	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough	697:773	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	1	39	theme	composite	175:183	arg1	membranes					195:203	composite nanofiber membranes	175:203	composite nanofiber membranes	175:203	Chitosan nanofibers (average diameter of 75nm) were electrospun on polyester (PET) scrim to form composite nanofiber membranes with controlled pore size.
26876857	1	40	theme	average	99:105	arg1	diameter					107:114	average diameter	99:114	average diameter of 75nm	99:122	Chitosan nanofibers (average diameter of 75nm) were electrospun on polyester (PET) scrim to form composite nanofiber membranes with controlled pore size.
26876857	5	41	theme	better	940:945	arg1	potential					947:955	the membranes' better potential	925:955	the membranes' better potential for dynamic adsorption	925:978	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	0	42	with	filtration	9:18	arg1	membranes					67:75	electrospun chitosan nanofiber membranes	36:75	electrospun chitosan nanofiber membranes	36:75	Chemical filtration of Cr (VI) with electrospun chitosan nanofiber membranes.
26876857	4	43	dep	Cr	659:660	arg1	concentration					667:679	concentration	667:679	concentration	667:679	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	4	43	dep	Cr	659:660	arg1	length					689:694	bed length	685:694	bed length	685:694	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	4	43	dep	Cr	659:660	arg1	VI					663:664	VI	663:664	VI	663:664	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	6	44	theme	deposition	1105:1114	arg1	density					1116:1122	nanofiber deposition density	1095:1122	nanofiber deposition density of 1g/m(2)	1095:1133	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	5	45	theme	nanofiber	898:906	arg1	mats					908:911	nanofiber mats	898:911	nanofiber mats	898:911	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	0	46	dep	Cr	23:24	arg1	VI					27:28	VI	27:28	VI	27:28	Chemical filtration of Cr (VI) with electrospun chitosan nanofiber membranes.
26876857	2	47	theme	chemical	286:293	arg1	filtration					295:304	chemical filtration	286:304	chemical filtration of Cr (VI) of 1-5mg/L	286:326	The membranes were then stacked as a membrane bed for chemical filtration of Cr (VI) of 1-5mg/L.
26876857	1	48	theme	nanofiber	185:193	arg1	membranes					195:203	composite nanofiber membranes	175:203	composite nanofiber membranes	175:203	Chitosan nanofibers (average diameter of 75nm) were electrospun on polyester (PET) scrim to form composite nanofiber membranes with controlled pore size.
26876857	5	49	dep	16.5mg-chromium/g-chitosan	791:816	arg1	16.5mg-chromium/g-chitosan					791:816	16.5mg-chromium/g-chitosan	791:816	16.5mg-chromium/g-chitosan	791:816	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	49	dep	16.5mg-chromium/g-chitosan	791:816	arg1	capacity					721:728	The maximum bed loading capacity	697:728	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough	697:773	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	49	dep	16.5mg-chromium/g-chitosan	791:816	arg1	higher					819:824	higher	819:824	higher	819:824	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	5	50	theme	dynamic	961:967	arg1	adsorption					969:978	dynamic adsorption	961:978	dynamic adsorption	961:978	The maximum bed loading capacity for 1mg/L Cr (VI) filtration at breakthrough was found to be 16.5mg-chromium/g-chitosan, higher than the static adsorption capacity of 11.0mg-chromium/g-chitosan using nanofiber mats, indicating the membranes' better potential for dynamic adsorption.
26876857	4	51	theme	bed	685:687	arg1	length					689:694	bed length	685:694	bed length	685:694	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	6	52	theme	membranes	1080:1088	arg1	layers					1062:1067	three layers	1056:1067	three layers of stacked membranes with nanofiber deposition density of 1g/m(2)	1056:1133	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	6	52	theme	membranes	1080:1088	arg1	length					997:1002	The minimum bed length	981:1002	The minimum bed length required to avoid breakthrough	981:1033	The minimum bed length required to avoid breakthrough was determined to be three layers of stacked membranes with nanofiber deposition density of 1g/m(2) by applying bed depth service time (BDST) model.
26876857	1	53	theme	75nm	119:122	arg1	diameter					107:114	average diameter	99:114	average diameter of 75nm	99:122	Chitosan nanofibers (average diameter of 75nm) were electrospun on polyester (PET) scrim to form composite nanofiber membranes with controlled pore size.
26876857	3	54	theme	bed	406:408	arg1	saturation					410:419	bed saturation	406:419	bed saturation	406:419	The performance of the bed with respect to loading capacity at breakthrough, bed saturation and utilization efficiency were carefully investigated.
26876857	0	55	theme	chitosan	48:55	arg1	membranes					67:75	electrospun chitosan nanofiber membranes	36:75	electrospun chitosan nanofiber membranes	36:75	Chemical filtration of Cr (VI) with electrospun chitosan nanofiber membranes.
26876857	1	56	dep	nanofibers	87:96	arg1	diameter					107:114	average diameter	99:114	average diameter of 75nm	99:122	Chitosan nanofibers (average diameter of 75nm) were electrospun on polyester (PET) scrim to form composite nanofiber membranes with controlled pore size.
26876857	4	57	theme	packed	585:590	arg1	pattern					592:598	packed pattern	585:598	packed pattern	585:598	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	0	58	theme	electrospun	36:46	arg1	membranes					67:75	electrospun chitosan nanofiber membranes	36:75	electrospun chitosan nanofiber membranes	36:75	Chemical filtration of Cr (VI) with electrospun chitosan nanofiber membranes.
26876857	4	59	theme	flow	552:555	arg1	rate					557:560	flow rate	552:560	flow rate	552:560	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	4	60	theme	membrane	607:614	arg1	distribution					568:579	flow distribution	563:579	flow distribution	563:579	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	4	60	theme	membrane	607:614	arg1	pattern					592:598	packed pattern	585:598	packed pattern	585:598	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	4	60	theme	membrane	607:614	arg1	pH					548:549	pH	548:549	pH	548:549	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	4	60	theme	membrane	607:614	arg1	rate					557:560	flow rate	552:560	flow rate	552:560	The results showed that while these three parameters were dependent on pH, flow rate, flow distribution and packed pattern of the membrane, the latter two were less affected by feed Cr (VI) concentration and bed length.
26876857	1	61	theme	controlled	210:219	arg1	size					226:229	controlled pore size	210:229	controlled pore size	210:229	Chitosan nanofibers (average diameter of 75nm) were electrospun on polyester (PET) scrim to form composite nanofiber membranes with controlled pore size.
24460772	2	0	theme	films	549:553	arg1	properties					528:537	the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties	451:537	the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films	451:553	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	9	1	theme	non-agglomerated	1680:1695	arg1	CNF					1716:1718	non-agglomerated and well dispersed CNF	1680:1718	non-agglomerated and well dispersed CNF	1680:1718	The scanning electron microscopy (SEM) images showed non-agglomerated and well dispersed CNF in the gluten matrix.
24460772	8	2	theme	MPa	1464:1466	arg1	TS					1452:1453	TS	1452:1453	TS of 3.721 MPa	1452:1466	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	8	2	theme	MPa	1464:1466	arg1	b					1474:1474	b	1474:1474	b	1474:1474	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	8	2	theme	MPa	1464:1466	arg1	ε					1472:1472	ε	1472:1472	ε(b) of 88.935%	1472:1486	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	5	3	theme	glycerol	1072:1079	arg1	concentration					1081:1093	glycerol concentration	1072:1093	glycerol concentration	1072:1093	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	5	3	theme	glycerol	1072:1079	arg1	conditions					957:966	The optimum conditions	945:966	The optimum conditions determined using response surface methodology (RSM)	945:1018	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	3	4	theme	regression	678:687	arg1	analysis					689:696	regression analysis	678:696	regression analysis	678:696	Through regression analysis, it was found that TS and ε(b) well fitted by quadratic polynomial equations (R² = 0.99 and 0.98, respectively) and the glycerol concentration was the most significant factor influencing them.
24460772	10	5	theme	transition	1874:1883	arg1	temperature					1885:1895	the glass transition temperature	1864:1895	the glass transition temperature (T(g))	1864:1902	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	1	6	theme	gluten	185:190	arg1	nanofibrils					218:228	gluten film containing cellulose nanofibrils	185:228	gluten film containing cellulose nanofibrils (CNF)	185:234	The aim of this investigation was the optimization of preparing gluten film containing cellulose nanofibrils (CNF).
24460772	1	6	theme	gluten	185:190	arg1	CNF					231:233	CNF	231:233	CNF	231:233	The aim of this investigation was the optimization of preparing gluten film containing cellulose nanofibrils (CNF).
24460772	10	7	theme	similar	2032:2038	arg1	behavior					2052:2059	similar degradation behavior	2032:2059	similar degradation behavior	2032:2059	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	1	8	theme	containing	197:206	arg1	nanofibrils					218:228	gluten film containing cellulose nanofibrils	185:228	gluten film containing cellulose nanofibrils (CNF)	185:234	The aim of this investigation was the optimization of preparing gluten film containing cellulose nanofibrils (CNF).
24460772	1	8	theme	containing	197:206	arg1	CNF					231:233	CNF	231:233	CNF	231:233	The aim of this investigation was the optimization of preparing gluten film containing cellulose nanofibrils (CNF).
24460772	10	9	theme	calorimetry	1764:1774	arg1	results					1782:1788	Differential scanning calorimetry (DSC) results	1742:1788	Differential scanning calorimetry (DSC) results	1742:1788	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	5	10	theme	optimum	949:955	arg1	concentration					1081:1093	glycerol concentration	1072:1093	glycerol concentration	1072:1093	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	5	10	theme	optimum	949:955	arg1	concentration					1119:1131	SDS concentration	1115:1131	SDS concentration	1115:1131	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	5	10	theme	optimum	949:955	arg1	g					1069:1069	11.129 g/100 g	1056:1069	11.129 g/100 g	1056:1069	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	5	10	theme	optimum	949:955	arg1	g					1109:1109	35.440 g/100 g	1096:1109	35.440 g/100 g	1096:1109	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	5	10	theme	optimum	949:955	arg1	conditions					957:966	The optimum conditions	945:966	The optimum conditions determined using response surface methodology (RSM)	945:1018	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	5	10	theme	optimum	949:955	arg1	concentration					1041:1053	CNF concentration	1037:1053	CNF concentration	1037:1053	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	0	11	from	matrix	87:92	arg1	properties					35:44	Thermomechanical and morphological properties	0:44	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.	0:119	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	0	11	from	matrix	87:92	arg1	films					63:67	nanocomposite films	49:67	nanocomposite films from wheat gluten matrix and cellulose nanofibrils	49:118	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	8	12	dep	predicted	1423:1431	arg1	TS					1452:1453	TS	1452:1453	TS of 3.721 MPa	1452:1466	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	8	12	dep	predicted	1423:1431	arg1	b					1474:1474	b	1474:1474	b	1474:1474	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	8	12	dep	predicted	1423:1431	arg1	ε					1472:1472	ε	1472:1472	ε(b) of 88.935%	1472:1486	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	2	13	theme	thermal	520:526	arg1	properties					528:537	the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties	451:537	the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films	451:553	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	14	theme	mechanical	647:656	arg1	properties					658:667	mechanical properties	647:667	mechanical properties	647:667	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	10	15	theme	nanocomposite	1915:1927	arg1	difference					1834:1843	any significant difference	1818:1843	any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C)	1818:1939	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	10	15	theme	nanocomposite	1915:1927	arg1	>					1848:1848	P > 0.05	1846:1853	P > 0.05	1846:1853	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	6	16	theme	predicted	1153:1161	arg1	responses					1163:1171	The predicted responses	1149:1171	The predicted responses for these film preparation conditions	1149:1209	The predicted responses for these film preparation conditions were a TS of 3.630 MPa and ε(b) of 86.033%.
24460772	6	16	theme	predicted	1153:1161	arg1	TS					1218:1219	a TS	1216:1219	a TS of 3.630 MPa	1216:1232	The predicted responses for these film preparation conditions were a TS of 3.630 MPa and ε(b) of 86.033%.
24460772	2	17	theme	central	269:275	arg1	CCD					295:297	CCD	295:297	CCD	295:297	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	17	theme	central	269:275	arg1	design					287:292	central composite design	269:292	central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations)	269:382	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	1	18	theme	investigation	137:149	arg1	aim					125:127	The aim	121:127	The aim of this investigation	121:149	The aim of this investigation was the optimization of preparing gluten film containing cellulose nanofibrils (CNF).
24460772	1	18	theme	investigation	137:149	arg1	optimization					159:170	the optimization	155:170	the optimization of preparing gluten film containing cellulose nanofibrils (CNF)	155:234	The aim of this investigation was the optimization of preparing gluten film containing cellulose nanofibrils (CNF).
24460772	2	19	with	design	287:292	arg1	glycerol					325:332	glycerol	325:332	glycerol	325:332	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	19	with	design	287:292	arg1	CNF					320:322	CNF	320:322	CNF	320:322	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	19	with	design	287:292	arg1	factors					311:317	three factors	305:317	three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations)	305:382	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	10	20	theme	control	1945:1951	arg1	°C					1966:1967	-29.64 °C	1959:1967	-29.64 °C	1959:1967	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	10	20	theme	control	1945:1951	arg1	film					1953:1956	control film	1945:1956	control film (-29.64 °C)	1945:1968	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	0	21	theme	wheat	74:78	arg1	matrix					87:92	wheat gluten matrix	74:92	wheat gluten matrix	74:92	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	6	22	theme	%	1252:1252	arg1	b					1240:1240	b	1240:1240	b	1240:1240	The predicted responses for these film preparation conditions were a TS of 3.630 MPa and ε(b) of 86.033%.
24460772	6	22	theme	%	1252:1252	arg1	ε					1238:1238	ε	1238:1238	ε(b) of 86.033%	1238:1252	The predicted responses for these film preparation conditions were a TS of 3.630 MPa and ε(b) of 86.033%.
24460772	6	22	theme	%	1252:1252	arg1	TS					1218:1219	a TS	1216:1219	a TS of 3.630 MPa	1216:1232	The predicted responses for these film preparation conditions were a TS of 3.630 MPa and ε(b) of 86.033%.
24460772	6	22	theme	%	1252:1252	arg1	responses					1163:1171	The predicted responses	1149:1171	The predicted responses for these film preparation conditions	1149:1209	The predicted responses for these film preparation conditions were a TS of 3.630 MPa and ε(b) of 86.033%.
24460772	10	23	theme	Differential	1742:1753	arg1	DSC					1777:1779	DSC	1777:1779	DSC	1777:1779	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	10	23	theme	Differential	1742:1753	arg1	calorimetry					1764:1774	Differential scanning calorimetry	1742:1774	Differential scanning calorimetry (DSC) results	1742:1788	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	3	24	theme	quadratic	744:752	arg1	equations					765:773	quadratic polynomial equations	744:773	quadratic polynomial equations	744:773	Through regression analysis, it was found that TS and ε(b) well fitted by quadratic polynomial equations (R² = 0.99 and 0.98, respectively) and the glycerol concentration was the most significant factor influencing them.
24460772	6	25	theme	MPa	1230:1232	arg1	b					1240:1240	b	1240:1240	b	1240:1240	The predicted responses for these film preparation conditions were a TS of 3.630 MPa and ε(b) of 86.033%.
24460772	6	25	theme	MPa	1230:1232	arg1	ε					1238:1238	ε	1238:1238	ε(b) of 86.033%	1238:1252	The predicted responses for these film preparation conditions were a TS of 3.630 MPa and ε(b) of 86.033%.
24460772	6	25	theme	MPa	1230:1232	arg1	TS					1218:1219	a TS	1216:1219	a TS of 3.630 MPa	1216:1232	The predicted responses for these film preparation conditions were a TS of 3.630 MPa and ε(b) of 86.033%.
24460772	6	25	theme	MPa	1230:1232	arg1	responses					1163:1171	The predicted responses	1149:1171	The predicted responses for these film preparation conditions	1149:1209	The predicted responses for these film preparation conditions were a TS of 3.630 MPa and ε(b) of 86.033%.
24460772	8	26	theme	variables	1609:1617	arg1	values					1619:1624	dependent variables values	1599:1624	dependent variables values	1599:1624	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	0	27	theme	cellulose	98:106	arg1	nanofibrils					108:118	cellulose nanofibrils	98:118	cellulose nanofibrils	98:118	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	5	28	theme	response	985:992	arg1	methodology					1002:1012	response surface methodology	985:1012	response surface methodology (RSM)	985:1018	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	5	28	theme	response	985:992	arg1	RSM					1015:1017	RSM	1015:1017	RSM	1015:1017	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	2	29	from	effect	421:426	arg1	properties					528:537	the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties	451:537	the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films	451:553	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	10	30	theme	thermogravimetric	1980:1996	arg1	TGA					2008:2010	TGA	2008:2010	TGA	2008:2010	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	10	30	theme	thermogravimetric	1980:1996	arg1	analysis					1998:2005	their thermogravimetric analysis	1974:2005	their thermogravimetric analysis (TGA)	1974:2011	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	0	31	theme	Thermomechanical	0:15	arg1	properties					35:44	Thermomechanical and morphological properties	0:44	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.	0:119	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	0	32	theme	morphological	21:33	arg1	properties					35:44	Thermomechanical and morphological properties	0:44	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.	0:119	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	7	33	theme	variables	1376:1384	arg1	values					1356:1361	predicted and actual values	1335:1361	predicted and actual values of dependent variables	1335:1384	The verification experiments were conducted under optimal conditions to compare predicted and actual values of dependent variables.
24460772	3	34	theme	glycerol	818:825	arg1	factor					866:871	the most significant factor	845:871	the most significant factor influencing them	845:888	Through regression analysis, it was found that TS and ε(b) well fitted by quadratic polynomial equations (R² = 0.99 and 0.98, respectively) and the glycerol concentration was the most significant factor influencing them.
24460772	3	34	theme	glycerol	818:825	arg1	concentration					827:839	the glycerol concentration	814:839	the glycerol concentration	814:839	Through regression analysis, it was found that TS and ε(b) well fitted by quadratic polynomial equations (R² = 0.99 and 0.98, respectively) and the glycerol concentration was the most significant factor influencing them.
24460772	2	35	theme	parameters	437:446	arg1	effect					421:426	the effect	417:426	the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films	417:553	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	9	36	theme	electron	1640:1647	arg1	SEM					1661:1663	SEM	1661:1663	SEM	1661:1663	The scanning electron microscopy (SEM) images showed non-agglomerated and well dispersed CNF in the gluten matrix.
24460772	9	36	theme	electron	1640:1647	arg1	microscopy					1649:1658	scanning electron microscopy	1631:1658	The scanning electron microscopy (SEM) images	1627:1671	The scanning electron microscopy (SEM) images showed non-agglomerated and well dispersed CNF in the gluten matrix.
24460772	0	37	theme	films	63:67	arg1	properties					35:44	Thermomechanical and morphological properties	0:44	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.	0:119	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	5	38	theme	CNF	1037:1039	arg1	g					1146:1146	6.259 g/100 g	1134:1146	6.259 g/100 g	1134:1146	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	5	38	theme	CNF	1037:1039	arg1	concentration					1041:1053	CNF concentration	1037:1053	CNF concentration	1037:1053	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	5	38	theme	CNF	1037:1039	arg1	conditions					957:966	The optimum conditions	945:966	The optimum conditions determined using response surface methodology (RSM)	945:1018	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	7	39	theme	actual	1349:1354	arg1	values					1356:1361	predicted and actual values	1335:1361	predicted and actual values of dependent variables	1335:1384	The verification experiments were conducted under optimal conditions to compare predicted and actual values of dependent variables.
24460772	10	40	theme	degradation	2040:2050	arg1	behavior					2052:2059	similar degradation behavior	2032:2059	similar degradation behavior	2032:2059	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	2	41	dep	mechanical	455:464	arg1	strength--TS					475:486	tensile strength--TS	467:486	tensile strength--TS	467:486	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	41	dep	mechanical	455:464	arg1	elongation					489:498	elongation	489:498	elongation at break--ε(b)	489:513	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	7	42	theme	predicted	1335:1343	arg1	values					1356:1361	predicted and actual values	1335:1361	predicted and actual values of dependent variables	1335:1384	The verification experiments were conducted under optimal conditions to compare predicted and actual values of dependent variables.
24460772	2	43	theme	sulfate	354:360	arg1	concentrations					368:381	sodium dodecyl sulfate (SDS) concentrations	339:381	sodium dodecyl sulfate (SDS) concentrations	339:381	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	44	theme	sodium	339:344	arg1	SDS					363:365	SDS	363:365	SDS	363:365	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	44	theme	sodium	339:344	arg1	sulfate					354:360	sodium dodecyl sulfate	339:360	sodium dodecyl sulfate (SDS) concentrations	339:381	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	7	45	theme	optimal	1305:1311	arg1	conditions					1313:1322	optimal conditions	1305:1322	optimal conditions	1305:1322	The verification experiments were conducted under optimal conditions to compare predicted and actual values of dependent variables.
24460772	2	46	theme	gluten	542:547	arg1	films					549:553	gluten films	542:553	gluten films	542:553	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	10	47	theme	glass	1868:1872	arg1	temperature					1885:1895	the glass transition temperature	1864:1895	the glass transition temperature (T(g))	1864:1902	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	10	48	dep	temperature	1885:1895	arg1	g					1900:1900	g	1900:1900	g	1900:1900	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	10	48	dep	temperature	1885:1895	arg1	T					1898:1898	T	1898:1898	T(g)	1898:1901	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	7	49	theme	verification	1259:1270	arg1	experiments					1272:1282	The verification experiments	1255:1282	The verification experiments	1255:1282	The verification experiments were conducted under optimal conditions to compare predicted and actual values of dependent variables.
24460772	9	50	theme	dispersed	1706:1714	arg1	CNF					1716:1718	non-agglomerated and well dispersed CNF	1680:1718	non-agglomerated and well dispersed CNF	1680:1718	The scanning electron microscopy (SEM) images showed non-agglomerated and well dispersed CNF in the gluten matrix.
24460772	10	51	theme	P	1846:1846	arg1	difference					1834:1843	any significant difference	1818:1843	any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C)	1818:1939	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	10	51	theme	P	1846:1846	arg1	>					1848:1848	P > 0.05	1846:1853	P > 0.05	1846:1853	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	1	52	theme	film	192:195	arg1	nanofibrils					218:228	gluten film containing cellulose nanofibrils	185:228	gluten film containing cellulose nanofibrils (CNF)	185:234	The aim of this investigation was the optimization of preparing gluten film containing cellulose nanofibrils (CNF).
24460772	1	52	theme	film	192:195	arg1	CNF					231:233	CNF	231:233	CNF	231:233	The aim of this investigation was the optimization of preparing gluten film containing cellulose nanofibrils (CNF).
24460772	10	53	theme	optimum	1907:1913	arg1	nanocomposite					1915:1927	optimum nanocomposite	1907:1927	optimum nanocomposite (-29.12 °C)	1907:1939	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	10	53	theme	optimum	1907:1913	arg1	°C					1937:1938	-29.12 °C	1930:1938	-29.12 °C	1930:1938	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	1	54	theme	cellulose	208:216	arg1	nanofibrils					218:228	gluten film containing cellulose nanofibrils	185:228	gluten film containing cellulose nanofibrils (CNF)	185:234	The aim of this investigation was the optimization of preparing gluten film containing cellulose nanofibrils (CNF).
24460772	1	54	theme	cellulose	208:216	arg1	CNF					231:233	CNF	231:233	CNF	231:233	The aim of this investigation was the optimization of preparing gluten film containing cellulose nanofibrils (CNF).
24460772	9	55	theme	gluten	1727:1732	arg1	matrix					1734:1739	the gluten matrix	1723:1739	the gluten matrix	1723:1739	The scanning electron microscopy (SEM) images showed non-agglomerated and well dispersed CNF in the gluten matrix.
24460772	10	56	theme	scanning	1755:1762	arg1	DSC					1777:1779	DSC	1777:1779	DSC	1777:1779	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	10	56	theme	scanning	1755:1762	arg1	calorimetry					1764:1774	Differential scanning calorimetry	1742:1774	Differential scanning calorimetry (DSC) results	1742:1788	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	6	57	theme	preparation	1188:1198	arg1	conditions					1200:1209	these film preparation conditions	1177:1209	these film preparation conditions	1177:1209	The predicted responses for these film preparation conditions were a TS of 3.630 MPa and ε(b) of 86.033%.
24460772	2	58	theme	optimization	240:251	arg1	procedure					253:261	An optimization procedure	237:261	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations)	237:382	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	8	59	theme	estimated	1534:1542	arg1	reasonable					1556:1565	reasonable	1556:1565	reasonable	1556:1565	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	8	59	theme	estimated	1534:1542	arg1	models					1544:1549	the estimated models	1530:1549	the estimated models	1530:1549	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	2	60	used	used	388:391	arg2	procedure					253:261	An optimization procedure	237:261	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations)	237:382	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	61	theme	composite	277:285	arg1	CCD					295:297	CCD	295:297	CCD	295:297	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	61	theme	composite	277:285	arg1	design					287:292	central composite design	269:292	central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations)	269:382	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	0	62	from	nanofibrils	108:118	arg1	properties					35:44	Thermomechanical and morphological properties	0:44	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.	0:119	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	0	62	from	nanofibrils	108:118	arg1	films					63:67	nanocomposite films	49:67	nanocomposite films from wheat gluten matrix and cellulose nanofibrils	49:118	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	10	63	theme	film	1953:1956	arg1	thermograms					2013:2023	control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms	1945:2023	control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms	1945:2023	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	2	64	dep	factors	311:317	arg1	glycerol					325:332	glycerol	325:332	glycerol	325:332	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	64	dep	factors	311:317	arg1	CNF					320:322	CNF	320:322	CNF	320:322	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	64	dep	factors	311:317	arg1	concentrations					368:381	sodium dodecyl sulfate (SDS) concentrations	339:381	sodium dodecyl sulfate (SDS) concentrations	339:381	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	64	dep	factors	311:317	arg1	factors					311:317	three factors	305:317	three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations)	305:382	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	0	65	theme	gluten	80:85	arg1	matrix					87:92	wheat gluten matrix	74:92	wheat gluten matrix	74:92	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	8	66	theme	high	1574:1577	arg1	accuracy					1579:1586	high accuracy	1574:1586	high accuracy	1574:1586	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	2	67	theme	mechanical	455:464	arg1	properties					528:537	the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties	451:537	the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films	451:553	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	0	68	from	properties	35:44	arg1	matrix					87:92	wheat gluten matrix	74:92	wheat gluten matrix	74:92	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	0	68	from	properties	35:44	arg1	nanofibrils					108:118	cellulose nanofibrils	98:118	cellulose nanofibrils	98:118	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	5	69	theme	SDS	1115:1117	arg1	conditions					957:966	The optimum conditions	945:966	The optimum conditions determined using response surface methodology (RSM)	945:1018	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	5	69	theme	SDS	1115:1117	arg1	concentration					1119:1131	SDS concentration	1115:1131	SDS concentration	1115:1131	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	3	70	theme	polynomial	754:763	arg1	equations					765:773	quadratic polynomial equations	744:773	quadratic polynomial equations	744:773	Through regression analysis, it was found that TS and ε(b) well fitted by quadratic polynomial equations (R² = 0.99 and 0.98, respectively) and the glycerol concentration was the most significant factor influencing them.
24460772	8	71	theme	dependent	1599:1607	arg1	variables					1609:1617	dependent variables	1599:1617	dependent variables values	1599:1624	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	10	72	theme	significant	1822:1832	arg1	difference					1834:1843	any significant difference	1818:1843	any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C)	1818:1939	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	10	72	theme	significant	1822:1832	arg1	>					1848:1848	P > 0.05	1846:1853	P > 0.05	1846:1853	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	5	73	theme	surface	994:1000	arg1	methodology					1002:1012	response surface methodology	985:1012	response surface methodology (RSM)	985:1018	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	5	73	theme	surface	994:1000	arg1	RSM					1015:1017	RSM	1015:1017	RSM	1015:1017	The optimum conditions determined using response surface methodology (RSM) were defined as: CNF concentration, 11.129 g/100 g, glycerol concentration, 35.440 g/100 g and SDS concentration, 6.259 g/100 g.
24460772	2	74	theme	tensile	467:473	arg1	strength--TS					475:486	tensile strength--TS	467:486	tensile strength--TS	467:486	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	74	theme	tensile	467:473	arg1	elongation					489:498	elongation	489:498	elongation at break--ε(b)	489:513	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	75	theme	mentioned	625:633	arg1	factors					635:641	mentioned factors	625:641	mentioned factors	625:641	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	76	dep	factors	635:641	arg1	the					621:623	the	621:623	the	621:623	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	0	77	theme	nanocomposite	49:61	arg1	films					63:67	nanocomposite films	49:67	nanocomposite films from wheat gluten matrix and cellulose nanofibrils	49:118	Thermomechanical and morphological properties of nanocomposite films from wheat gluten matrix and cellulose nanofibrils.
24460772	9	78	theme	scanning	1631:1638	arg1	SEM					1661:1663	SEM	1661:1663	SEM	1661:1663	The scanning electron microscopy (SEM) images showed non-agglomerated and well dispersed CNF in the gluten matrix.
24460772	9	78	theme	scanning	1631:1638	arg1	microscopy					1649:1658	scanning electron microscopy	1631:1658	The scanning electron microscopy (SEM) images	1627:1671	The scanning electron microscopy (SEM) images showed non-agglomerated and well dispersed CNF in the gluten matrix.
24460772	7	79	theme	dependent	1366:1374	arg1	variables					1376:1384	dependent variables	1366:1384	dependent variables	1366:1384	The verification experiments were conducted under optimal conditions to compare predicted and actual values of dependent variables.
24460772	9	80	theme	microscopy	1649:1658	arg1	images					1666:1671	The scanning electron microscopy (SEM) images	1627:1671	The scanning electron microscopy (SEM) images	1627:1671	The scanning electron microscopy (SEM) images showed non-agglomerated and well dispersed CNF in the gluten matrix.
24460772	8	81	theme	actual	1437:1442	arg1	values					1444:1449	actual values	1437:1449	actual values	1437:1449	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	10	82	theme	analysis	1998:2005	arg1	thermograms					2013:2023	control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms	1945:2023	control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms	1945:2023	Differential scanning calorimetry (DSC) results indicated that there is not any significant difference (P > 0.05) between the glass transition temperature (T(g)) of optimum nanocomposite (-29.12 °C) and control film (-29.64 °C) and their thermogravimetric analysis (TGA) thermograms showed similar degradation behavior.
24460772	3	83	theme	significant	854:864	arg1	factor					866:871	the most significant factor	845:871	the most significant factor influencing them	845:888	Through regression analysis, it was found that TS and ε(b) well fitted by quadratic polynomial equations (R² = 0.99 and 0.98, respectively) and the glycerol concentration was the most significant factor influencing them.
24460772	3	83	theme	significant	854:864	arg1	concentration					827:839	the glycerol concentration	814:839	the glycerol concentration	814:839	Through regression analysis, it was found that TS and ε(b) well fitted by quadratic polynomial equations (R² = 0.99 and 0.98, respectively) and the glycerol concentration was the most significant factor influencing them.
24460772	2	84	from	break--ε	503:510	arg1	strength--TS					475:486	tensile strength--TS	467:486	tensile strength--TS	467:486	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	84	from	break--ε	503:510	arg1	elongation					489:498	elongation	489:498	elongation at break--ε(b)	489:513	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	85	theme	dodecyl	346:352	arg1	SDS					363:365	SDS	363:365	SDS	363:365	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	2	85	theme	dodecyl	346:352	arg1	sulfate					354:360	sodium dodecyl sulfate	339:360	sodium dodecyl sulfate (SDS) concentrations	339:381	An optimization procedure using central composite design (CCD) with three factors (CNF, glycerol, and sodium dodecyl sulfate (SDS) concentrations) was used in order to investigate the effect of these parameters on the mechanical (tensile strength--TS, elongation at break--ε(b)) and thermal properties of gluten films and to establish a formulation to depict the relationship between the mentioned factors and mechanical properties.
24460772	8	86	theme	%	1486:1486	arg1	TS					1452:1453	TS	1452:1453	TS of 3.721 MPa	1452:1466	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	8	86	theme	%	1486:1486	arg1	b					1474:1474	b	1474:1474	b	1474:1474	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
24460772	8	86	theme	%	1486:1486	arg1	ε					1472:1472	ε	1472:1472	ε(b) of 88.935%	1472:1486	This experiment indicated that both predicted and actual values (TS of 3.721 MPa and ε(b) of 88.935%) almost coincide each other and therefore the estimated models were reasonable and of high accuracy to predict dependent variables values.
28867228	7	0	theme	significant	1246:1256	arg1	difference					1258:1267	significant difference	1246:1267	significant difference	1246:1267	Although TiO2 NPs alone were effective more than negative control in reduction of promastigots and amastigotes but they didn't show significant difference compared with free glucantime (p>0.05).
28867228	7	1	from	reduction	1183:1191	arg1	effective					1143:1151	effective	1143:1151	effective	1143:1151	Although TiO2 NPs alone were effective more than negative control in reduction of promastigots and amastigotes but they didn't show significant difference compared with free glucantime (p>0.05).
28867228	5	2	theme	tripan	876:881	arg1	test					892:895	tripan blue dye test	876:895	tripan blue dye test	876:895	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies was studied by tripan blue dye test and their anti-amastigotes effect by counting the average number of parasites per infected J774 macrophages in 100 cells.
28867228	3	3	theme	NPs	530:532	arg1	amounts					534:540	TiO2 NPs amounts	525:540	TiO2 NPs amounts	525:540	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	8	4	theme	72h	1360:1362	arg1	exposure					1364:1371	72h exposure	1360:1371	72h exposure	1360:1371	However, at the concentration of 50μg/mL and after 72h exposure nanoassemblies decreased the proliferation of L. major promastigotes and amastigotes 13 and 4-fold, respectively compared with glucantime alone.
28867228	0	5	theme	major	105:109	arg1	promastigote					63:74	promastigote	63:74	promastigote	63:74	Chitosan-titanium dioxide-glucantime nanoassemblies effects on promastigote and amastigote of Leishmania major.
28867228	6	6	theme	6mg	1100:1102	arg1	NPs					1109:1111	6mg TiO2 NPs	1100:1111	6mg TiO2 NPs	1100:1111	The optimized formulation obtained by using 12.5mg glucantime, 25mg chitosan and 6mg TiO2 NPs.
28867228	7	7	theme	negative	1163:1170	arg1	control					1172:1178	negative control	1163:1178	negative control	1163:1178	Although TiO2 NPs alone were effective more than negative control in reduction of promastigots and amastigotes but they didn't show significant difference compared with free glucantime (p>0.05).
28867228	1	8	theme	chitosan-titanium	177:193	arg1	TiO2					204:207	TiO2	204:207	TiO2	204:207	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	1	8	theme	chitosan-titanium	177:193	arg1	dioxide					195:201	chitosan-titanium dioxide	177:201	chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites	177:357	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	8	9	theme	L.	1419:1420	arg1	promastigotes					1428:1440	L. major promastigotes	1419:1440	L. major promastigotes	1419:1440	However, at the concentration of 50μg/mL and after 72h exposure nanoassemblies decreased the proliferation of L. major promastigotes and amastigotes 13 and 4-fold, respectively compared with glucantime alone.
28867228	1	10	from	effects	313:319	arg1	parasites					349:357	Leishmania parasites	338:357	Leishmania parasites	338:357	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	3	11	theme	particle	562:569	arg1	size					571:574	particle size	562:574	particle size	562:574	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	3	12	from	size	571:574	arg1	nanoassemblies					649:662	nanoassemblies	649:662	nanoassemblies	649:662	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	1	13	theme	synergistic	269:279	arg1	effects					281:287	their synergistic effects	263:287	their synergistic effects	263:287	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	3	14	from	nanoassemblies	649:662	arg1	efficiency					625:634	release efficiency	617:634	release efficiency	617:634	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	3	14	from	nanoassemblies	649:662	arg1	size					571:574	particle size	562:574	particle size	562:574	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	3	14	from	nanoassemblies	649:662	arg1	potential					582:590	zeta potential	577:590	zeta potential	577:590	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	3	14	from	nanoassemblies	649:662	arg1	efficiency					601:610	loading efficiency	593:610	loading efficiency	593:610	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	3	14	from	nanoassemblies	649:662	arg1	drug					639:642	drug	639:642	drug from nanoassemblies	639:662	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	7	15	theme	free	1283:1286	arg1	p>0.05					1300:1305	p>0.05	1300:1305	p>0.05	1300:1305	Although TiO2 NPs alone were effective more than negative control in reduction of promastigots and amastigotes but they didn't show significant difference compared with free glucantime (p>0.05).
28867228	7	15	theme	free	1283:1286	arg1	glucantime					1288:1297	free glucantime	1283:1297	free glucantime (p>0.05)	1283:1306	Although TiO2 NPs alone were effective more than negative control in reduction of promastigots and amastigotes but they didn't show significant difference compared with free glucantime (p>0.05).
28867228	6	16	theme	optimized	1023:1031	arg1	formulation					1033:1043	The optimized formulation	1019:1043	The optimized formulation	1019:1043	The optimized formulation obtained by using 12.5mg glucantime, 25mg chitosan and 6mg TiO2 NPs.
28867228	4	17	theme	UV	725:726	arg1	spectroscopy					728:739	UV spectroscopy	725:739	UV spectroscopy	725:739	The conjugation of TiO2/chitosan-glucantime was verified by UV spectroscopy and changes in surface charge of NPs.
28867228	8	18	dep	respectively	1473:1484	arg1	glucantime					1500:1509	glucantime	1500:1509	glucantime alone	1500:1515	However, at the concentration of 50μg/mL and after 72h exposure nanoassemblies decreased the proliferation of L. major promastigotes and amastigotes 13 and 4-fold, respectively compared with glucantime alone.
28867228	5	19	theme	blue	883:886	arg1	test					892:895	tripan blue dye test	876:895	tripan blue dye test	876:895	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies was studied by tripan blue dye test and their anti-amastigotes effect by counting the average number of parasites per infected J774 macrophages in 100 cells.
28867228	3	20	theme	glucantime	500:509	arg1	effects					489:495	The effects	485:495	The effects of glucantime, chitosan and TiO2 NPs amounts	485:540	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	3	21	from	efficiency	625:634	arg1	nanoassemblies					649:662	nanoassemblies	649:662	nanoassemblies	649:662	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	1	22	theme	dioxide	195:201	arg1	NPs					225:227	NPs	225:227	NPs	225:227	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	1	22	theme	dioxide	195:201	arg1	nanoparticles					210:222	chitosan-titanium dioxide (TiO2) nanoparticles	177:222	chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites	177:357	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	5	23	theme	average	947:953	arg1	number					955:960	the average number	943:960	the average number of parasites per infected J774 macrophages	943:1003	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies was studied by tripan blue dye test and their anti-amastigotes effect by counting the average number of parasites per infected J774 macrophages in 100 cells.
28867228	4	24	from	changes	745:751	arg1	charge					764:769	surface charge	756:769	surface charge of NPs	756:776	The conjugation of TiO2/chitosan-glucantime was verified by UV spectroscopy and changes in surface charge of NPs.
28867228	7	25	theme	amastigotes	1213:1223	arg1	reduction					1183:1191	reduction	1183:1191	reduction of promastigots and amastigotes	1183:1223	Although TiO2 NPs alone were effective more than negative control in reduction of promastigots and amastigotes but they didn't show significant difference compared with free glucantime (p>0.05).
28867228	7	26	theme	TiO2	1123:1126	arg1	NPs					1128:1130	TiO2 NPs	1123:1130	TiO2 NPs	1123:1130	Although TiO2 NPs alone were effective more than negative control in reduction of promastigots and amastigotes but they didn't show significant difference compared with free glucantime (p>0.05).
28867228	5	27	theme	infected	979:986	arg1	macrophages					993:1003	infected J774 macrophages	979:1003	infected J774 macrophages	979:1003	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies was studied by tripan blue dye test and their anti-amastigotes effect by counting the average number of parasites per infected J774 macrophages in 100 cells.
28867228	5	28	theme	anti-amastigotes	907:922	arg1	effect					924:929	their anti-amastigotes effect	901:929	their anti-amastigotes effect	901:929	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies was studied by tripan blue dye test and their anti-amastigotes effect by counting the average number of parasites per infected J774 macrophages in 100 cells.
28867228	0	29	theme	Chitosan-titanium	0:16	arg1	dioxide-glucantime					18:35	Chitosan-titanium dioxide-glucantime	0:35	Chitosan-titanium dioxide-glucantime	0:35	Chitosan-titanium dioxide-glucantime nanoassemblies effects on promastigote and amastigote of Leishmania major.
28867228	0	30	dep	major	105:109	arg1	Leishmania					94:103	Leishmania major	94:109	Leishmania major	94:109	Chitosan-titanium dioxide-glucantime nanoassemblies effects on promastigote and amastigote of Leishmania major.
28867228	2	31	theme	electrostatic	396:408	arg1	interactions					410:421	electrostatic interactions	396:421	electrostatic interactions	396:421	The nanoassemblies were prepared by electrostatic interactions and optimized by a response surface central composite design.
28867228	2	32	theme	composite	467:475	arg1	design					477:482	a response surface central composite design	440:482	a response surface central composite design	440:482	The nanoassemblies were prepared by electrostatic interactions and optimized by a response surface central composite design.
28867228	8	33	theme	major	1422:1426	arg1	promastigotes					1428:1440	L. major promastigotes	1419:1440	L. major promastigotes	1419:1440	However, at the concentration of 50μg/mL and after 72h exposure nanoassemblies decreased the proliferation of L. major promastigotes and amastigotes 13 and 4-fold, respectively compared with glucantime alone.
28867228	3	34	from	efficiency	601:610	arg1	nanoassemblies					649:662	nanoassemblies	649:662	nanoassemblies	649:662	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	2	35	theme	surface	451:457	arg1	design					477:482	a response surface central composite design	440:482	a response surface central composite design	440:482	The nanoassemblies were prepared by electrostatic interactions and optimized by a response surface central composite design.
28867228	2	36	theme	central	459:465	arg1	design					477:482	a response surface central composite design	440:482	a response surface central composite design	440:482	The nanoassemblies were prepared by electrostatic interactions and optimized by a response surface central composite design.
28867228	5	37	theme	J774	988:991	arg1	macrophages					993:1003	infected J774 macrophages	979:1003	infected J774 macrophages	979:1003	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies was studied by tripan blue dye test and their anti-amastigotes effect by counting the average number of parasites per infected J774 macrophages in 100 cells.
28867228	3	38	theme	zeta	577:580	arg1	potential					582:590	zeta potential	577:590	zeta potential	577:590	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	2	39	theme	response	442:449	arg1	design					477:482	a response surface central composite design	440:482	a response surface central composite design	440:482	The nanoassemblies were prepared by electrostatic interactions and optimized by a response surface central composite design.
28867228	5	40	theme	glucantime	811:820	arg1	effect					801:806	The anti-promastigots effect	779:806	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies	779:859	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies was studied by tripan blue dye test and their anti-amastigotes effect by counting the average number of parasites per infected J774 macrophages in 100 cells.
28867228	1	41	theme	toxic	307:311	arg1	effects					313:319	the toxic effects	303:319	the toxic effects of glucantime on Leishmania parasites	303:357	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	4	42	theme	TiO2/chitosan-glucantime	684:707	arg1	conjugation					669:679	The conjugation	665:679	The conjugation of TiO2/chitosan-glucantime	665:707	The conjugation of TiO2/chitosan-glucantime was verified by UV spectroscopy and changes in surface charge of NPs.
28867228	5	43	theme	dye	888:890	arg1	test					892:895	tripan blue dye test	876:895	tripan blue dye test	876:895	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies was studied by tripan blue dye test and their anti-amastigotes effect by counting the average number of parasites per infected J774 macrophages in 100 cells.
28867228	7	44	from	effective	1143:1151	arg1	reduction					1183:1191	reduction	1183:1191	reduction of promastigots and amastigotes	1183:1223	Although TiO2 NPs alone were effective more than negative control in reduction of promastigots and amastigotes but they didn't show significant difference compared with free glucantime (p>0.05).
28867228	8	45	theme	50μg/mL	1342:1348	arg1	concentration					1325:1337	the concentration	1321:1337	the concentration of 50μg/mL	1321:1348	However, at the concentration of 50μg/mL and after 72h exposure nanoassemblies decreased the proliferation of L. major promastigotes and amastigotes 13 and 4-fold, respectively compared with glucantime alone.
28867228	7	46	theme	promastigots	1196:1207	arg1	reduction					1183:1191	reduction	1183:1191	reduction of promastigots and amastigotes	1183:1223	Although TiO2 NPs alone were effective more than negative control in reduction of promastigots and amastigotes but they didn't show significant difference compared with free glucantime (p>0.05).
28867228	6	47	theme	25mg	1082:1085	arg1	chitosan					1087:1094	25mg chitosan	1082:1094	25mg chitosan	1082:1094	The optimized formulation obtained by using 12.5mg glucantime, 25mg chitosan and 6mg TiO2 NPs.
28867228	6	48	theme	TiO2	1104:1107	arg1	NPs					1109:1111	6mg TiO2 NPs	1100:1111	6mg TiO2 NPs	1100:1111	The optimized formulation obtained by using 12.5mg glucantime, 25mg chitosan and 6mg TiO2 NPs.
28867228	3	49	theme	loading	593:599	arg1	efficiency					601:610	loading efficiency	593:610	loading efficiency	593:610	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	4	50	from	spectroscopy	728:739	arg1	charge					764:769	surface charge	756:769	surface charge of NPs	756:776	The conjugation of TiO2/chitosan-glucantime was verified by UV spectroscopy and changes in surface charge of NPs.
28867228	1	51	theme	present	131:137	arg1	study					139:143	the present study	127:143	the present study	127:143	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	5	52	theme	TiO2/chitosan	832:844	arg1	nanoassemblies					846:859	TiO2/chitosan nanoassemblies	832:859	TiO2/chitosan nanoassemblies	832:859	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies was studied by tripan blue dye test and their anti-amastigotes effect by counting the average number of parasites per infected J774 macrophages in 100 cells.
28867228	1	53	theme	glucantime	324:333	arg1	effects					313:319	the toxic effects	303:319	the toxic effects of glucantime on Leishmania parasites	303:357	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	1	54	theme	Leishmania	338:347	arg1	parasites					349:357	Leishmania parasites	338:357	Leishmania parasites	338:357	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	8	55	theme	amastigotes	1446:1456	arg1	proliferation					1402:1414	the proliferation	1398:1414	the proliferation of L. major promastigotes and amastigotes 13	1398:1459	However, at the concentration of 50μg/mL and after 72h exposure nanoassemblies decreased the proliferation of L. major promastigotes and amastigotes 13 and 4-fold, respectively compared with glucantime alone.
28867228	3	56	theme	amounts	534:540	arg1	effects					489:495	The effects	485:495	The effects of glucantime, chitosan and TiO2 NPs amounts	485:540	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	1	57	theme	study	139:143	arg1	purpose					116:122	The purpose	112:122	The purpose of the present study	112:143	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	8	58	theme	promastigotes	1428:1440	arg1	proliferation					1402:1414	the proliferation	1398:1414	the proliferation of L. major promastigotes and amastigotes 13	1398:1459	However, at the concentration of 50μg/mL and after 72h exposure nanoassemblies decreased the proliferation of L. major promastigotes and amastigotes 13 and 4-fold, respectively compared with glucantime alone.
28867228	4	59	theme	surface	756:762	arg1	charge					764:769	surface charge	756:769	surface charge of NPs	756:776	The conjugation of TiO2/chitosan-glucantime was verified by UV spectroscopy and changes in surface charge of NPs.
28867228	3	60	theme	drug	639:642	arg1	efficiency					625:634	release efficiency	617:634	release efficiency	617:634	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	3	60	theme	drug	639:642	arg1	size					571:574	particle size	562:574	particle size	562:574	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	3	60	theme	drug	639:642	arg1	potential					582:590	zeta potential	577:590	zeta potential	577:590	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	3	60	theme	drug	639:642	arg1	efficiency					601:610	loading efficiency	593:610	loading efficiency	593:610	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	4	61	theme	NPs	774:776	arg1	charge					764:769	surface charge	756:769	surface charge of NPs	756:776	The conjugation of TiO2/chitosan-glucantime was verified by UV spectroscopy and changes in surface charge of NPs.
28867228	3	62	theme	chitosan	512:519	arg1	effects					489:495	The effects	485:495	The effects of glucantime, chitosan and TiO2 NPs amounts	485:540	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	3	63	from	potential	582:590	arg1	nanoassemblies					649:662	nanoassemblies	649:662	nanoassemblies	649:662	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	5	64	theme	parasites	965:973	arg1	number					955:960	the average number	943:960	the average number of parasites per infected J774 macrophages	943:1003	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies was studied by tripan blue dye test and their anti-amastigotes effect by counting the average number of parasites per infected J774 macrophages in 100 cells.
28867228	3	65	theme	release	617:623	arg1	efficiency					625:634	release efficiency	617:634	release efficiency	617:634	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28867228	1	66	theme	nanoparticles	210:222	arg1	nanoassemblies					159:172	nanoassemblies	159:172	nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites	159:357	The purpose of the present study was to design nanoassemblies of chitosan-titanium dioxide (TiO2) nanoparticles (NPs) loaded with glucantime for using their synergistic effects and enhancing the toxic effects of glucantime on Leishmania parasites.
28867228	6	67	theme	12.5mg	1063:1068	arg1	glucantime					1070:1079	12.5mg glucantime	1063:1079	12.5mg glucantime	1063:1079	The optimized formulation obtained by using 12.5mg glucantime, 25mg chitosan and 6mg TiO2 NPs.
28867228	5	68	theme	anti-promastigots	783:799	arg1	effect					801:806	The anti-promastigots effect	779:806	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies	779:859	The anti-promastigots effect of glucantime loaded in TiO2/chitosan nanoassemblies was studied by tripan blue dye test and their anti-amastigotes effect by counting the average number of parasites per infected J774 macrophages in 100 cells.
28867228	0	69	from	effects	52:58	arg1	promastigote					63:74	promastigote	63:74	promastigote	63:74	Chitosan-titanium dioxide-glucantime nanoassemblies effects on promastigote and amastigote of Leishmania major.
28867228	0	69	from	effects	52:58	arg1	amastigote					80:89	amastigote	80:89	amastigote	80:89	Chitosan-titanium dioxide-glucantime nanoassemblies effects on promastigote and amastigote of Leishmania major.
28867228	7	70	dep	effective	1143:1151	arg1	Although					1114:1121	Although	1114:1121	Although	1114:1121	Although TiO2 NPs alone were effective more than negative control in reduction of promastigots and amastigotes but they didn't show significant difference compared with free glucantime (p>0.05).
28867228	3	71	theme	TiO2	525:528	arg1	amounts					534:540	TiO2 NPs amounts	525:540	TiO2 NPs amounts	525:540	The effects of glucantime, chitosan and TiO2 NPs amounts were studied on the particle size, zeta potential, loading efficiency, and release efficiency of drug from nanoassemblies.
28445626	8	0	theme	release	1037:1043	arg1	model					1045:1049	two-step release model	1028:1049	two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2	1028:1118	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	4	1	theme	crystalline	608:618	arg1	nature					620:625	highly crystalline nature	601:625	highly crystalline nature of CNWs	601:633	X-ray powder diffraction analysis confirmed highly crystalline nature of CNWs with 92.81% crystallinity.
28445626	11	2	theme	dressing	1514:1521	arg1	applications					1523:1534	wound dressing applications	1508:1534	wound dressing applications with sustained drug release	1508:1562	Overall, the performed studies confirmed CS-PVP-CNWs based release system can as a potential candidate for wound dressing applications with sustained drug release.
28445626	5	3	theme	thermal	697:703	arg1	properties					720:729	the thermal and mechanical properties	693:729	the thermal and mechanical properties of films	693:738	Incorporation of CNWs enhanced the thermal and mechanical properties of films.
28445626	9	4	theme	different	1242:1250	arg1	rates					1260:1264	different release rates	1242:1264	different release rates	1242:1264	The release kinetics confirmed biphasic release profile with different release rates along with diffusion controlled curcumin release.
28445626	7	5	theme	sustained	934:942	arg1	release					949:955	sustained drug release	934:955	sustained drug release from polymeric matrix	934:977	Films prepared with CNWs showed improved swelling behavior which resulted in sustained drug release from polymeric matrix.
28445626	1	6	theme	incorporated	271:282	arg1	CNWs					308:311	CNWs	308:311	CNWs	308:311	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	1	6	theme	incorporated	271:282	arg1	nanowhiskers					294:305	incorporated cellulose nanowhiskers	271:305	incorporated cellulose nanowhiskers (CNWs)	271:312	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	2	7	theme	cellulose	386:394	arg1	hydrolysis					372:381	acid hydrolysis	367:381	acid hydrolysis of cellulose with sulfuric acid	367:413	CNWs were prepared by acid hydrolysis of cellulose with sulfuric acid.
28445626	1	8	theme	film	210:213	arg1	preparation					185:195	the preparation	181:195	the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application	181:342	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	11	9	theme	performed	1414:1422	arg1	studies					1424:1430	the performed studies	1410:1430	the performed studies confirmed CS-PVP-CNWs based release system	1410:1473	Overall, the performed studies confirmed CS-PVP-CNWs based release system can as a potential candidate for wound dressing applications with sustained drug release.
28445626	0	10	theme	drug	133:136	arg1	application					147:157	wound healing drug delivery application	119:157	wound healing drug delivery application	119:157	Fabrication and characterization of chitosan, polyvinylpyrrolidone, and cellulose nanowhiskers nanocomposite films for wound healing drug delivery application.
28445626	9	11	theme	controlled	1287:1296	arg1	release					1307:1313	diffusion controlled curcumin release	1277:1313	diffusion controlled curcumin release	1277:1313	The release kinetics confirmed biphasic release profile with different release rates along with diffusion controlled curcumin release.
28445626	7	12	theme	improved	889:896	arg1	behavior					907:914	improved swelling behavior	889:914	improved swelling behavior which resulted in sustained drug release from polymeric matrix	889:977	Films prepared with CNWs showed improved swelling behavior which resulted in sustained drug release from polymeric matrix.
28445626	8	13	from	surface	1088:1094	arg1	desorption					1062:1071	desorption	1062:1071	desorption from the outer surface of the film	1062:1106	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	11	14	theme	based	1454:1458	arg1	system					1468:1473	CS-PVP-CNWs based release system	1442:1473	CS-PVP-CNWs based release system	1442:1473	Overall, the performed studies confirmed CS-PVP-CNWs based release system can as a potential candidate for wound dressing applications with sustained drug release.
28445626	5	15	theme	mechanical	709:718	arg1	properties					720:729	the thermal and mechanical properties	693:729	the thermal and mechanical properties of films	693:738	Incorporation of CNWs enhanced the thermal and mechanical properties of films.
28445626	3	16	theme	emission	422:429	arg1	microscopy					449:458	Field emission scanning electron microscopy	416:458	Field emission scanning electron microscopy studies	416:466	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	10	17	with	biocompatibility	1343:1358	arg1	activities					1389:1398	excellent antibacterial activities	1365:1398	excellent antibacterial activities	1365:1398	Prepared films showed high biocompatibility with excellent antibacterial activities.
28445626	8	18	dep	In	980:981	arg1	vitro					983:987	vitro	983:987	vitro	983:987	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	10	19	theme	Prepared	1316:1323	arg1	films					1325:1329	Prepared films	1316:1329	Prepared films	1316:1329	Prepared films showed high biocompatibility with excellent antibacterial activities.
28445626	11	20	theme	drug	1551:1554	arg1	release					1556:1562	sustained drug release	1541:1562	sustained drug release	1541:1562	Overall, the performed studies confirmed CS-PVP-CNWs based release system can as a potential candidate for wound dressing applications with sustained drug release.
28445626	2	21	theme	sulfuric	401:408	arg1	acid					410:413	sulfuric acid	401:413	sulfuric acid	401:413	CNWs were prepared by acid hydrolysis of cellulose with sulfuric acid.
28445626	0	22	theme	wound	119:123	arg1	healing					125:131	wound healing	119:131	wound healing drug delivery application	119:157	Fabrication and characterization of chitosan, polyvinylpyrrolidone, and cellulose nanowhiskers nanocomposite films for wound healing drug delivery application.
28445626	3	23	theme	scanning	431:438	arg1	microscopy					449:458	Field emission scanning electron microscopy	416:458	Field emission scanning electron microscopy studies	416:466	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	1	24	theme	cellulose	284:292	arg1	CNWs					308:311	CNWs	308:311	CNWs	308:311	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	1	24	theme	cellulose	284:292	arg1	nanowhiskers					294:305	incorporated cellulose nanowhiskers	271:305	incorporated cellulose nanowhiskers (CNWs)	271:312	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	8	25	theme	In	980:981	arg1	data					1006:1009	In vitro curcumin release data	980:1009	In vitro curcumin release data	980:1009	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	8	25	theme	In	980:981	arg1	diffusion					1123:1131	diffusion	1123:1131	diffusion from within the film and subsequent desorption	1123:1178	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	4	26	theme	powder	563:568	arg1	diffraction					570:580	X-ray powder diffraction	557:580	X-ray powder diffraction analysis	557:589	X-ray powder diffraction analysis confirmed highly crystalline nature of CNWs with 92.81% crystallinity.
28445626	6	27	dep	Fourier	741:747	arg1	transform					749:757	transform	749:757	transform infrared spectroscopy data	749:784	Fourier transform infrared spectroscopy data showed physical interactions between polymer-polymer and polymer-drug.
28445626	8	28	theme	curcumin	989:996	arg1	data					1006:1009	In vitro curcumin release data	980:1009	In vitro curcumin release data	980:1009	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	8	28	theme	curcumin	989:996	arg1	diffusion					1123:1131	diffusion	1123:1131	diffusion from within the film and subsequent desorption	1123:1178	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	13	29	dep	105A	1623:1626	arg1	2391-2404					1629:1637	2391-2404	1629:1637	2391-2404	1629:1637	J Biomed Mater Res Part A: 105A: 2391-2404, 2017.
28445626	3	30	theme	Field	416:420	arg1	microscopy					449:458	Field emission scanning electron microscopy	416:458	Field emission scanning electron microscopy studies	416:466	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	0	31	theme	chitosan	36:43	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of chitosan, polyvinylpyrrolidone, and cellulose nanowhiskers nanocomposite films for wound healing drug delivery application.
28445626	0	31	theme	chitosan	36:43	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of chitosan, polyvinylpyrrolidone, and cellulose nanowhiskers nanocomposite films for wound healing drug delivery application.
28445626	1	32	with	chitosan	221:228	arg1	CNWs					308:311	CNWs	308:311	CNWs	308:311	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	1	32	with	chitosan	221:228	arg1	nanowhiskers					294:305	incorporated cellulose nanowhiskers	271:305	incorporated cellulose nanowhiskers (CNWs)	271:312	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	3	33	theme	20-30	513:517	arg1	nm					519:520	nm	519:520	nm	519:520	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	0	34	theme	polyvinylpyrrolidone	46:65	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of chitosan, polyvinylpyrrolidone, and cellulose nanowhiskers nanocomposite films for wound healing drug delivery application.
28445626	0	34	theme	polyvinylpyrrolidone	46:65	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of chitosan, polyvinylpyrrolidone, and cellulose nanowhiskers nanocomposite films for wound healing drug delivery application.
28445626	1	35	theme	drug	318:321	arg1	application					332:342	drug delivery application	318:342	drug delivery application	318:342	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	6	36	theme	physical	793:800	arg1	interactions					802:813	physical interactions	793:813	physical interactions between polymer-polymer and polymer-drug	793:854	Fourier transform infrared spectroscopy data showed physical interactions between polymer-polymer and polymer-drug.
28445626	3	37	theme	CNWs	503:506	arg1	morphology					489:498	nanofibrous morphology	477:498	nanofibrous morphology of CNWs with 20-30 nm diameter	477:529	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	3	37	theme	CNWs	503:506	arg1	nm					543:544	200-250 nm	535:544	200-250 nm in length	535:554	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	2	38	with	hydrolysis	372:381	arg1	acid					410:413	sulfuric acid	401:413	sulfuric acid	401:413	CNWs were prepared by acid hydrolysis of cellulose with sulfuric acid.
28445626	8	39	theme	two-step	1028:1035	arg1	model					1045:1049	two-step release model	1028:1049	two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2	1028:1118	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	4	40	theme	CNWs	630:633	arg1	nature					620:625	highly crystalline nature	601:625	highly crystalline nature of CNWs	601:633	X-ray powder diffraction analysis confirmed highly crystalline nature of CNWs with 92.81% crystallinity.
28445626	4	41	theme	92.81	640:644	arg1	%					645:645	%	645:645	%	645:645	X-ray powder diffraction analysis confirmed highly crystalline nature of CNWs with 92.81% crystallinity.
28445626	5	42	theme	CNWs	679:682	arg1	Incorporation					662:674	Incorporation	662:674	Incorporation of CNWs	662:682	Incorporation of CNWs enhanced the thermal and mechanical properties of films.
28445626	9	43	theme	release	1221:1227	arg1	profile					1229:1235	biphasic release profile	1212:1235	biphasic release profile	1212:1235	The release kinetics confirmed biphasic release profile with different release rates along with diffusion controlled curcumin release.
28445626	11	44	theme	wound	1508:1512	arg1	applications					1523:1534	wound dressing applications	1508:1534	wound dressing applications with sustained drug release	1508:1562	Overall, the performed studies confirmed CS-PVP-CNWs based release system can as a potential candidate for wound dressing applications with sustained drug release.
28445626	3	45	with	CNWs	503:506	arg1	diameter					522:529	20-30 nm diameter	513:529	20-30 nm diameter	513:529	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	3	46	from	nm	543:544	arg1	length					549:554	length	549:554	length	549:554	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	7	47	theme	drug	944:947	arg1	release					949:955	sustained drug release	934:955	sustained drug release from polymeric matrix	934:977	Films prepared with CNWs showed improved swelling behavior which resulted in sustained drug release from polymeric matrix.
28445626	1	48	theme	composite	200:208	arg1	film					210:213	composite film	200:213	composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application	200:342	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	8	49	theme	outer	1082:1086	arg1	surface					1088:1094	the outer surface	1078:1094	the outer surface of the film	1078:1106	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	8	50	dep	film	1149:1152	arg1	the					1145:1147	the	1145:1147	the	1145:1147	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	0	51	theme	delivery	138:145	arg1	application					147:157	wound healing drug delivery application	119:157	wound healing drug delivery application	119:157	Fabrication and characterization of chitosan, polyvinylpyrrolidone, and cellulose nanowhiskers nanocomposite films for wound healing drug delivery application.
28445626	9	52	theme	diffusion	1277:1285	arg1	release					1307:1313	diffusion controlled curcumin release	1277:1313	diffusion controlled curcumin release	1277:1313	The release kinetics confirmed biphasic release profile with different release rates along with diffusion controlled curcumin release.
28445626	11	53	theme	release	1460:1466	arg1	system					1468:1473	CS-PVP-CNWs based release system	1442:1473	CS-PVP-CNWs based release system	1442:1473	Overall, the performed studies confirmed CS-PVP-CNWs based release system can as a potential candidate for wound dressing applications with sustained drug release.
28445626	9	54	theme	curcumin	1298:1305	arg1	release					1307:1313	diffusion controlled curcumin release	1277:1313	diffusion controlled curcumin release	1277:1313	The release kinetics confirmed biphasic release profile with different release rates along with diffusion controlled curcumin release.
28445626	7	55	theme	swelling	898:905	arg1	behavior					907:914	improved swelling behavior	889:914	improved swelling behavior which resulted in sustained drug release from polymeric matrix	889:977	Films prepared with CNWs showed improved swelling behavior which resulted in sustained drug release from polymeric matrix.
28445626	13	56	dep	Res	1611:1613	arg1	105A					1623:1626	105A	1623:1626	105A	1623:1626	J Biomed Mater Res Part A: 105A: 2391-2404, 2017.
28445626	13	56	dep	Res	1611:1613	arg1	A					1620:1620	Part A	1615:1620	J Biomed Mater Res Part A: 105A: 2391-2404, 2017.	1596:1644	J Biomed Mater Res Part A: 105A: 2391-2404, 2017.
28445626	12	57	dep	©	1565:1565	arg1	Inc.					1591:1594	Inc.	1591:1594	Inc.	1591:1594	© 2017 Wiley Periodicals, Inc.
28445626	5	58	theme	films	734:738	arg1	properties					720:729	the thermal and mechanical properties	693:729	the thermal and mechanical properties of films	693:738	Incorporation of CNWs enhanced the thermal and mechanical properties of films.
28445626	11	59	with	applications	1523:1534	arg1	release					1556:1562	sustained drug release	1541:1562	sustained drug release	1541:1562	Overall, the performed studies confirmed CS-PVP-CNWs based release system can as a potential candidate for wound dressing applications with sustained drug release.
28445626	8	60	dep	model	1045:1049	arg1	Step					1113:1116	Step 2	1113:1118	Step 2	1113:1118	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	8	60	dep	model	1045:1049	arg1	Step					1052:1055	Step 1	1052:1057	Step 1 as desorption from the outer surface of the film	1052:1106	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	13	61	theme	Part	1615:1618	arg1	A					1620:1620	Part A	1615:1620	J Biomed Mater Res Part A: 105A: 2391-2404, 2017.	1596:1644	J Biomed Mater Res Part A: 105A: 2391-2404, 2017.
28445626	9	62	theme	release	1185:1191	arg1	kinetics					1193:1200	The release kinetics	1181:1200	The release kinetics	1181:1200	The release kinetics confirmed biphasic release profile with different release rates along with diffusion controlled curcumin release.
28445626	0	63	theme	cellulose	72:80	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of chitosan, polyvinylpyrrolidone, and cellulose nanowhiskers nanocomposite films for wound healing drug delivery application.
28445626	0	63	theme	cellulose	72:80	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of chitosan, polyvinylpyrrolidone, and cellulose nanowhiskers nanocomposite films for wound healing drug delivery application.
28445626	10	64	theme	high	1338:1341	arg1	biocompatibility					1343:1358	high biocompatibility	1338:1358	high biocompatibility with excellent antibacterial activities	1338:1398	Prepared films showed high biocompatibility with excellent antibacterial activities.
28445626	0	65	theme	nanocomposite	95:107	arg1	films					109:113	nanocomposite films	95:113	nanocomposite films for wound healing drug delivery application	95:157	Fabrication and characterization of chitosan, polyvinylpyrrolidone, and cellulose nanowhiskers nanocomposite films for wound healing drug delivery application.
28445626	8	66	theme	film	1103:1106	arg1	surface					1088:1094	the outer surface	1078:1094	the outer surface of the film	1078:1106	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	1	67	with	polyvinylpyrrolidone	239:258	arg1	CNWs					308:311	CNWs	308:311	CNWs	308:311	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	1	67	with	polyvinylpyrrolidone	239:258	arg1	nanowhiskers					294:305	incorporated cellulose nanowhiskers	271:305	incorporated cellulose nanowhiskers (CNWs)	271:312	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	11	68	theme	sustained	1541:1549	arg1	release					1556:1562	sustained drug release	1541:1562	sustained drug release	1541:1562	Overall, the performed studies confirmed CS-PVP-CNWs based release system can as a potential candidate for wound dressing applications with sustained drug release.
28445626	0	69	theme	healing	125:131	arg1	application					147:157	wound healing drug delivery application	119:157	wound healing drug delivery application	119:157	Fabrication and characterization of chitosan, polyvinylpyrrolidone, and cellulose nanowhiskers nanocomposite films for wound healing drug delivery application.
28445626	10	70	theme	antibacterial	1375:1387	arg1	activities					1389:1398	excellent antibacterial activities	1365:1398	excellent antibacterial activities	1365:1398	Prepared films showed high biocompatibility with excellent antibacterial activities.
28445626	7	71	from	matrix	972:977	arg1	release					949:955	sustained drug release	934:955	sustained drug release from polymeric matrix	934:977	Films prepared with CNWs showed improved swelling behavior which resulted in sustained drug release from polymeric matrix.
28445626	4	72	theme	diffraction	570:580	arg1	analysis					582:589	X-ray powder diffraction analysis	557:589	X-ray powder diffraction analysis	557:589	X-ray powder diffraction analysis confirmed highly crystalline nature of CNWs with 92.81% crystallinity.
28445626	6	73	dep	transform	749:757	arg1	infrared					759:766	infrared	759:766	transform infrared spectroscopy data	749:784	Fourier transform infrared spectroscopy data showed physical interactions between polymer-polymer and polymer-drug.
28445626	3	74	theme	electron	440:447	arg1	microscopy					449:458	Field emission scanning electron microscopy	416:458	Field emission scanning electron microscopy studies	416:466	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	10	75	theme	excellent	1365:1373	arg1	activities					1389:1398	excellent antibacterial activities	1365:1398	excellent antibacterial activities	1365:1398	Prepared films showed high biocompatibility with excellent antibacterial activities.
28445626	3	76	theme	microscopy	449:458	arg1	studies					460:466	Field emission scanning electron microscopy studies	416:466	Field emission scanning electron microscopy studies	416:466	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	3	77	theme	nanofibrous	477:487	arg1	morphology					489:498	nanofibrous morphology	477:498	nanofibrous morphology of CNWs with 20-30 nm diameter	477:529	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	8	78	theme	release	998:1004	arg1	data					1006:1009	In vitro curcumin release data	980:1009	In vitro curcumin release data	980:1009	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	8	78	theme	release	998:1004	arg1	diffusion					1123:1131	diffusion	1123:1131	diffusion from within the film and subsequent desorption	1123:1178	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	4	79	theme	X-ray	557:561	arg1	diffraction					570:580	X-ray powder diffraction	557:580	X-ray powder diffraction analysis	557:589	X-ray powder diffraction analysis confirmed highly crystalline nature of CNWs with 92.81% crystallinity.
28445626	3	80	from	morphology	489:498	arg1	length					549:554	length	549:554	length	549:554	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	3	81	theme	nm	519:520	arg1	diameter					522:529	20-30 nm diameter	513:529	20-30 nm diameter	513:529	Field emission scanning electron microscopy studies revealed nanofibrous morphology of CNWs with 20-30 nm diameter and 200-250 nm in length.
28445626	1	82	theme	delivery	323:330	arg1	application					332:342	drug delivery application	318:342	drug delivery application	318:342	This study describes the preparation of composite film using chitosan (CS) and polyvinylpyrrolidone (PVP) with incorporated cellulose nanowhiskers (CNWs) for drug delivery application.
28445626	8	83	theme	subsequent	1158:1167	arg1	desorption					1169:1178	subsequent desorption	1158:1178	subsequent desorption	1158:1178	In vitro curcumin release data were fitted with two-step release model; Step 1 as desorption from the outer surface of the film, and Step 2 as diffusion from within the film and subsequent desorption.
28445626	6	84	theme	spectroscopy	768:779	arg1	data					781:784	spectroscopy data	768:784	spectroscopy data	768:784	Fourier transform infrared spectroscopy data showed physical interactions between polymer-polymer and polymer-drug.
28445626	9	85	theme	release	1252:1258	arg1	rates					1260:1264	different release rates	1242:1264	different release rates	1242:1264	The release kinetics confirmed biphasic release profile with different release rates along with diffusion controlled curcumin release.
28445626	4	86	theme	%	645:645	arg1	crystallinity					647:659	92.81% crystallinity	640:659	92.81% crystallinity	640:659	X-ray powder diffraction analysis confirmed highly crystalline nature of CNWs with 92.81% crystallinity.
28445626	11	87	theme	potential	1484:1492	arg1	candidate					1494:1502	a potential candidate	1482:1502	a potential candidate for wound dressing applications with sustained drug release	1482:1562	Overall, the performed studies confirmed CS-PVP-CNWs based release system can as a potential candidate for wound dressing applications with sustained drug release.
28445626	9	88	theme	biphasic	1212:1219	arg1	profile					1229:1235	biphasic release profile	1212:1235	biphasic release profile	1212:1235	The release kinetics confirmed biphasic release profile with different release rates along with diffusion controlled curcumin release.
28445626	2	89	theme	acid	367:370	arg1	hydrolysis					372:381	acid hydrolysis	367:381	acid hydrolysis of cellulose with sulfuric acid	367:413	CNWs were prepared by acid hydrolysis of cellulose with sulfuric acid.
28445626	7	90	theme	polymeric	962:970	arg1	matrix					972:977	polymeric matrix	962:977	polymeric matrix	962:977	Films prepared with CNWs showed improved swelling behavior which resulted in sustained drug release from polymeric matrix.
29243216	0	0	theme	Hepatitis	90:98	arg1	virus					150:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus	90:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV)	90:160	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	0	0	theme	Hepatitis	90:98	arg1	HBV					157:159	HBV	157:159	HBV	157:159	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	1	1	theme	estimated	338:346	arg1	340					348:350	an estimated 340	335:350	an estimated 340 million chronic HBV cases	335:376	Moreover, an estimated 340 million chronic HBV cases are in need of treatment.
29243216	5	2	theme	marketed	1047:1054	arg1	vaccine					1056:1062	the marketed vaccine	1043:1062	the marketed vaccine (Genvac B) administered intramuscularly	1043:1102	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	5	2	theme	marketed	1047:1054	arg1	B					1072:1072	Genvac B	1065:1072	Genvac B	1065:1072	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	6	3	theme	nanometric	1115:1124	arg1	morphology					1126:1135	nanometric morphology	1115:1135	nanometric morphology	1115:1135	SFNs with nanometric morphology and structural similarity with chylomicrons assist in improved uptake and processing to lymphatics.
29243216	2	4	theme	vaccine	464:470	arg1	formulation					472:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	Development of safe, stable, and more effective hepatitis B vaccine formulation would address these challenges.
29243216	1	5	theme	340	348:350	arg1	cases					372:376	an estimated 340 million chronic HBV cases	335:376	an estimated 340 million chronic HBV cases	335:376	Moreover, an estimated 340 million chronic HBV cases are in need of treatment.
29243216	2	6	theme	formulation	472:482	arg1	Development					404:414	Development	404:414	Development of safe, stable, and more effective hepatitis B vaccine formulation	404:482	Development of safe, stable, and more effective hepatitis B vaccine formulation would address these challenges.
29243216	9	7	theme	vaccine	1520:1526	arg1	formulation					1528:1538	this vaccine formulation	1515:1538	this vaccine formulation	1515:1538	Improved cellular immune response induced by this vaccine formulation suggests that it could be tested as an immunotherapeutic vaccine as well.
29243216	0	8	theme	Traditional	103:113	arg1	virus					150:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus	90:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV)	90:160	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	0	8	theme	Traditional	103:113	arg1	HBV					157:159	HBV	157:159	HBV	157:159	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	1	9	theme	million	352:358	arg1	cases					372:376	an estimated 340 million chronic HBV cases	335:376	an estimated 340 million chronic HBV cases	335:376	Moreover, an estimated 340 million chronic HBV cases are in need of treatment.
29243216	0	10	theme	disease	200:206	arg1	burden					208:213	the disease burden	196:213	the disease burden	196:213	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	5	11	theme	significant	981:991	arg1	response					1022:1029	MPLA-induced significant higher humoral, and cellular response	968:1029	MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly	968:1102	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	3	12	theme	surface	540:546	arg1	rHBsAg					557:562	rHBsAg	557:562	rHBsAg	557:562	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	12	theme	surface	540:546	arg1	antigen					548:554	Recombinant hepatitis B surface antigen	516:554	Recombinant hepatitis B surface antigen (rHBsAg)	516:563	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	5	13	theme	rHBsAg	941:946	arg1	1 μg					933:936	1 μg	933:936	1 μg of rHBsAg	933:946	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	3	14	theme	composite	762:770	arg1	design					772:777	central composite design	754:777	central composite design (CCD)	754:783	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	14	theme	composite	762:770	arg1	CCD					780:782	CCD	780:782	CCD	780:782	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	0	15	theme	B.	100:101	arg1	virus					150:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus	90:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV)	90:160	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	0	15	theme	B.	100:101	arg1	HBV					157:159	HBV	157:159	HBV	157:159	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	1	16	theme	chronic	360:366	arg1	cases					372:376	an estimated 340 million chronic HBV cases	335:376	an estimated 340 million chronic HBV cases	335:376	Moreover, an estimated 340 million chronic HBV cases are in need of treatment.
29243216	0	17	theme	antibody	259:266	arg1	titers					268:273	seroprotective antibody titers	244:273	seroprotective antibody titers	244:273	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	6	18	theme	structural	1141:1150	arg1	similarity					1152:1161	structural similarity	1141:1161	structural similarity with chylomicrons	1141:1179	SFNs with nanometric morphology and structural similarity with chylomicrons assist in improved uptake and processing to lymphatics.
29243216	7	19	theme	signals	1382:1388	arg1	induction					1362:1370	induction	1362:1370	induction of danger signals	1362:1388	Moreover, the presence of an immunogenic component in its structure further augments delivery of rHBsAg to immune cells with induction of danger signals.
29243216	0	20	theme	recombinant	126:136	arg1	virus					150:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus	90:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV)	90:160	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	0	20	theme	recombinant	126:136	arg1	HBV					157:159	HBV	157:159	HBV	157:159	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	1	21	theme	HBV	368:370	arg1	cases					372:376	an estimated 340 million chronic HBV cases	335:376	an estimated 340 million chronic HBV cases	335:376	Moreover, an estimated 340 million chronic HBV cases are in need of treatment.
29243216	7	22	theme	immune	1344:1349	arg1	cells					1351:1355	immune cells	1344:1355	immune cells	1344:1355	Moreover, the presence of an immunogenic component in its structure further augments delivery of rHBsAg to immune cells with induction of danger signals.
29243216	0	23	theme	seroprotective	244:257	arg1	titers					268:273	seroprotective antibody titers	244:273	seroprotective antibody titers	244:273	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	3	24	theme	hepatitis	528:536	arg1	rHBsAg					557:562	rHBsAg	557:562	rHBsAg	557:562	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	24	theme	hepatitis	528:536	arg1	antigen					548:554	Recombinant hepatitis B surface antigen	516:554	Recombinant hepatitis B surface antigen (rHBsAg)	516:563	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	5	25	contain	containing	922:931	arg2	1 μg					933:936	1 μg	933:936	1 μg of rHBsAg	933:946	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	5	25	contain	containing	922:931	arg1	formulation					910:920	formulation	910:920	formulation containing 1 μg of rHBsAg	910:946	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	0	26	theme	parenteral	115:124	arg1	virus					150:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus	90:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV)	90:160	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	0	26	theme	parenteral	115:124	arg1	HBV					157:159	HBV	157:159	HBV	157:159	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	2	27	theme	B	462:462	arg1	formulation					472:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	Development of safe, stable, and more effective hepatitis B vaccine formulation would address these challenges.
29243216	3	28	theme	B	538:538	arg1	rHBsAg					557:562	rHBsAg	557:562	rHBsAg	557:562	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	28	theme	B	538:538	arg1	antigen					548:554	Recombinant hepatitis B surface antigen	516:554	Recombinant hepatitis B surface antigen (rHBsAg)	516:563	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	5	29	theme	Genvac	1065:1070	arg1	vaccine					1056:1062	the marketed vaccine	1043:1062	the marketed vaccine (Genvac B) administered intramuscularly	1043:1102	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	5	29	theme	Genvac	1065:1070	arg1	B					1072:1072	Genvac B	1065:1072	Genvac B	1065:1072	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	4	30	theme	immune	790:795	arg1	potential					797:805	Its immune potential	786:805	Its immune potential	786:805	Its immune potential was evaluated with preset immunization protocol in a murine model.
29243216	5	31	theme	Dose	874:877	arg1	study					890:894	Dose escalation study	874:894	Dose escalation study	874:894	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	4	32	theme	preset	826:831	arg1	protocol					846:853	preset immunization protocol	826:853	preset immunization protocol	826:853	Its immune potential was evaluated with preset immunization protocol in a murine model.
29243216	0	33	theme	Lipid	0:4	arg1	vaccines					162:169	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines	0:169	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines	0:169	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	3	34	theme	response	712:719	arg1	RSM					742:744	RSM	742:744	RSM	742:744	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	34	theme	response	712:719	arg1	methodology					729:739	response surface methodology	712:739	response surface methodology (RSM)	712:745	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	7	35	from	presence	1251:1258	arg1	structure					1295:1303	its structure	1291:1303	its structure	1291:1303	Moreover, the presence of an immunogenic component in its structure further augments delivery of rHBsAg to immune cells with induction of danger signals.
29243216	5	36	theme	escalation	879:888	arg1	study					890:894	Dose escalation study	874:894	Dose escalation study	874:894	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	0	37	theme	Chylomicron	19:29	arg1	vaccines					162:169	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines	0:169	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines	0:169	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	3	38	theme	surface	721:727	arg1	RSM					742:744	RSM	742:744	RSM	742:744	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	38	theme	surface	721:727	arg1	methodology					729:739	response surface methodology	712:739	response surface methodology (RSM)	712:745	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	0	39	theme	B	148:148	arg1	virus					150:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus	90:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV)	90:160	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	0	39	theme	B	148:148	arg1	HBV					157:159	HBV	157:159	HBV	157:159	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	6	40	theme	improved	1191:1198	arg1	uptake					1200:1205	improved uptake	1191:1205	improved uptake	1191:1205	SFNs with nanometric morphology and structural similarity with chylomicrons assist in improved uptake and processing to lymphatics.
29243216	2	41	theme	safe	419:422	arg1	formulation					472:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	Development of safe, stable, and more effective hepatitis B vaccine formulation would address these challenges.
29243216	0	42	theme	adjuvanted	8:17	arg1	Chylomicron					19:29	A adjuvanted Chylomicron	6:29	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines	0:169	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	6	43	with	similarity	1152:1161	arg1	chylomicrons					1168:1179	chylomicrons	1168:1179	chylomicrons	1168:1179	SFNs with nanometric morphology and structural similarity with chylomicrons assist in improved uptake and processing to lymphatics.
29243216	6	43	with	similarity	1152:1161	arg1	morphology					1126:1135	nanometric morphology	1115:1135	nanometric morphology	1115:1135	SFNs with nanometric morphology and structural similarity with chylomicrons assist in improved uptake and processing to lymphatics.
29243216	0	44	theme	hepatitis	138:146	arg1	virus					150:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus	90:154	Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV)	90:160	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	0	44	theme	hepatitis	138:146	arg1	HBV					157:159	HBV	157:159	HBV	157:159	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	8	45	theme	new	1435:1437	arg1	prospect					1439:1446	new prospect	1435:1446	new prospect for the dose sparing	1435:1467	This multi-adjuvant based approach explores new prospect for the dose sparing.
29243216	7	46	theme	danger	1375:1380	arg1	signals					1382:1388	danger signals	1375:1388	danger signals	1375:1388	Moreover, the presence of an immunogenic component in its structure further augments delivery of rHBsAg to immune cells with induction of danger signals.
29243216	7	47	attach	presence	1251:1258	arg1	structure					1295:1303	its structure	1291:1303	its structure	1291:1303	Moreover, the presence of an immunogenic component in its structure further augments delivery of rHBsAg to immune cells with induction of danger signals.
29243216	7	47	attach	presence	1251:1258	arg2	component					1278:1286	an immunogenic component	1263:1286	an immunogenic component	1263:1286	Moreover, the presence of an immunogenic component in its structure further augments delivery of rHBsAg to immune cells with induction of danger signals.
29243216	8	48	theme	dose	1456:1459	arg1	sparing					1461:1467	the dose sparing	1452:1467	the dose sparing	1452:1467	This multi-adjuvant based approach explores new prospect for the dose sparing.
29243216	0	49	theme	Solid	41:45	arg1	Nanoemulsions					51:63	Solid Fat Nanoemulsions	41:63	Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV)	41:160	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	9	50	theme	immunotherapeutic	1579:1595	arg1	vaccine					1597:1603	an immunotherapeutic vaccine	1576:1603	an immunotherapeutic vaccine as well	1576:1611	Improved cellular immune response induced by this vaccine formulation suggests that it could be tested as an immunotherapeutic vaccine as well.
29243216	9	50	theme	immunotherapeutic	1579:1595	arg1	it					1554:1555	it	1554:1555	it	1554:1555	Improved cellular immune response induced by this vaccine formulation suggests that it could be tested as an immunotherapeutic vaccine as well.
29243216	2	51	theme	hepatitis	452:460	arg1	formulation					472:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	Development of safe, stable, and more effective hepatitis B vaccine formulation would address these challenges.
29243216	6	52	with	SFNs	1105:1108	arg1	chylomicrons					1168:1179	chylomicrons	1168:1179	chylomicrons	1168:1179	SFNs with nanometric morphology and structural similarity with chylomicrons assist in improved uptake and processing to lymphatics.
29243216	6	52	with	SFNs	1105:1108	arg1	morphology					1126:1135	nanometric morphology	1115:1135	nanometric morphology	1115:1135	SFNs with nanometric morphology and structural similarity with chylomicrons assist in improved uptake and processing to lymphatics.
29243216	3	53	theme	lipid	632:636	arg1	MPLA					641:644	MPLA	641:644	MPLA	641:644	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	53	theme	lipid	632:636	arg1	A					638:638	monophosphoryl lipid A	617:638	monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM)	617:745	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	2	54	theme	stable	425:430	arg1	formulation					472:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	Development of safe, stable, and more effective hepatitis B vaccine formulation would address these challenges.
29243216	7	55	theme	immunogenic	1266:1276	arg1	component					1278:1286	an immunogenic component	1263:1286	an immunogenic component	1263:1286	Moreover, the presence of an immunogenic component in its structure further augments delivery of rHBsAg to immune cells with induction of danger signals.
29243216	2	56	theme	effective	442:450	arg1	formulation					472:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	safe, stable, and more effective hepatitis B vaccine formulation	419:482	Development of safe, stable, and more effective hepatitis B vaccine formulation would address these challenges.
29243216	4	57	theme	immunization	833:844	arg1	protocol					846:853	preset immunization protocol	826:853	preset immunization protocol	826:853	Its immune potential was evaluated with preset immunization protocol in a murine model.
29243216	3	58	theme	solid	575:579	arg1	nanoemulsions					585:597	solid fat nanoemulsions	575:597	solid fat nanoemulsions (SFNs)	575:604	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	58	theme	solid	575:579	arg1	SFNs					600:603	SFNs	600:603	SFNs	600:603	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	0	59	theme	vaccinated	284:293	arg1	non-responders					308:321	non-responders	308:321	non-responders	308:321	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	0	59	theme	vaccinated	284:293	arg1	individuals					295:305	5-10% vaccinated individuals	278:305	5-10% vaccinated individuals (non-responders)	278:322	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	9	60	theme	Improved	1470:1477	arg1	response					1495:1502	Improved cellular immune response	1470:1502	Improved cellular immune response induced by this vaccine formulation	1470:1538	Improved cellular immune response induced by this vaccine formulation suggests that it could be tested as an immunotherapeutic vaccine as well.
29243216	0	61	theme	Fat	47:49	arg1	Nanoemulsions					51:63	Solid Fat Nanoemulsions	41:63	Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV)	41:160	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	4	62	theme	murine	860:865	arg1	model					867:871	a murine model	858:871	a murine model	858:871	Its immune potential was evaluated with preset immunization protocol in a murine model.
29243216	5	63	theme	humoral	1000:1006	arg1	response					1022:1029	MPLA-induced significant higher humoral, and cellular response	968:1029	MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly	968:1102	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	3	64	theme	fat	581:583	arg1	nanoemulsions					585:597	solid fat nanoemulsions	575:597	solid fat nanoemulsions (SFNs)	575:604	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	64	theme	fat	581:583	arg1	SFNs					600:603	SFNs	600:603	SFNs	600:603	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	9	65	theme	cellular	1479:1486	arg1	response					1495:1502	Improved cellular immune response	1470:1502	Improved cellular immune response induced by this vaccine formulation	1470:1538	Improved cellular immune response induced by this vaccine formulation suggests that it could be tested as an immunotherapeutic vaccine as well.
29243216	8	66	theme	multi-adjuvant	1396:1409	arg1	approach					1417:1424	This multi-adjuvant based approach	1391:1424	This multi-adjuvant based approach	1391:1424	This multi-adjuvant based approach explores new prospect for the dose sparing.
29243216	0	67	dep	vaccinated	284:293	arg1	%					282:282	5-10%	278:282	5-10%	278:282	Lipid A adjuvanted Chylomicron Mimicking Solid Fat Nanoemulsions for Immunization Against Hepatitis B. Traditional parenteral recombinant hepatitis B virus (HBV) vaccines have effectively reduced the disease burden despite being able to induce seroprotective antibody titers in 5-10% vaccinated individuals (non-responders).
29243216	3	68	dep	design	772:777	arg1	i.e.					748:751	i.e.	748:751	i.e.	748:751	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	69	theme	central	754:760	arg1	design					772:777	central composite design	754:777	central composite design (CCD)	754:783	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	69	theme	central	754:760	arg1	CCD					780:782	CCD	780:782	CCD	780:782	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	5	70	theme	MPLA-induced	968:979	arg1	response					1022:1029	MPLA-induced significant higher humoral, and cellular response	968:1029	MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly	968:1102	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	9	71	theme	immune	1488:1493	arg1	response					1495:1502	Improved cellular immune response	1470:1502	Improved cellular immune response induced by this vaccine formulation	1470:1538	Improved cellular immune response induced by this vaccine formulation suggests that it could be tested as an immunotherapeutic vaccine as well.
29243216	3	72	theme	monophosphoryl	617:630	arg1	MPLA					641:644	MPLA	641:644	MPLA	641:644	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	72	theme	monophosphoryl	617:630	arg1	A					638:638	monophosphoryl lipid A	617:638	monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM)	617:745	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	1	73	dep	treatment	393:401	arg1	need					385:388	need	385:388	need	385:388	Moreover, an estimated 340 million chronic HBV cases are in need of treatment.
29243216	5	74	theme	cellular	1013:1020	arg1	response					1022:1029	MPLA-induced significant higher humoral, and cellular response	968:1029	MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly	968:1102	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	7	75	theme	component	1278:1286	arg1	presence					1251:1258	the presence	1247:1258	the presence of an immunogenic component in its structure	1247:1303	Moreover, the presence of an immunogenic component in its structure further augments delivery of rHBsAg to immune cells with induction of danger signals.
29243216	5	76	with	SFNs	958:961	arg1	response					1022:1029	MPLA-induced significant higher humoral, and cellular response	968:1029	MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly	968:1102	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	7	77	theme	rHBsAg	1334:1339	arg1	delivery					1322:1329	delivery	1322:1329	delivery of rHBsAg to immune cells	1322:1355	Moreover, the presence of an immunogenic component in its structure further augments delivery of rHBsAg to immune cells with induction of danger signals.
29243216	8	78	theme	based	1411:1415	arg1	approach					1417:1424	This multi-adjuvant based approach	1391:1424	This multi-adjuvant based approach	1391:1424	This multi-adjuvant based approach explores new prospect for the dose sparing.
29243216	3	79	theme	Recombinant	516:526	arg1	rHBsAg					557:562	rHBsAg	557:562	rHBsAg	557:562	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	79	theme	Recombinant	516:526	arg1	antigen					548:554	Recombinant hepatitis B surface antigen	516:554	Recombinant hepatitis B surface antigen (rHBsAg)	516:563	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	5	80	dep	humoral	1000:1006	arg1	higher					993:998	higher	993:998	higher	993:998	Dose escalation study revealed that formulation containing 1 μg of rHBsAg entrapped SFNs with MPLA-induced significant higher humoral, and cellular response compared to the marketed vaccine (Genvac B) administered intramuscularly.
29243216	3	81	contain	containing	606:615	arg1	nanoemulsions					585:597	solid fat nanoemulsions	575:597	solid fat nanoemulsions (SFNs)	575:604	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	81	contain	containing	606:615	arg2	MPLA					641:644	MPLA	641:644	MPLA	641:644	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	81	contain	containing	606:615	arg1	SFNs					600:603	SFNs	600:603	SFNs	600:603	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
29243216	3	81	contain	containing	606:615	arg2	A					638:638	monophosphoryl lipid A	617:638	monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM)	617:745	Recombinant hepatitis B surface antigen (rHBsAg) entrapped solid fat nanoemulsions (SFNs) containing monophosphoryl lipid A (MPLA) that was prepared and optimized by quality by design (QbD) using response surface methodology (RSM), i.e., central composite design (CCD).
25861570	5	0	theme	NMR	1011:1013	arg1	combination					954:964	a combination	952:964	a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts	952:1044	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	0	1	from	Elucidation	11:21	arg1	O181					77:80	Escherichia coli O181	60:80	Escherichia coli O181	60:80	Structural Elucidation of the O-Antigen Polysaccharide from Escherichia coli O181.
25861570	7	2	theme	proton	1462:1467	arg1	resonances					1469:1478	the five anomeric proton resonances	1444:1478	the five anomeric proton resonances	1444:1478	Both the OS and PSs exhibit rare conformational behavior since two of the five anomeric proton resonances could only be observed at an elevated temperature.
25861570	1	3	theme	food-borne	168:177	arg1	infection					179:187	food-borne infection	168:187	food-borne infection in humans	168:197	Shiga-toxin-producing Escherichia coli (STEC) is an important pathogen associated to food-borne infection in humans; strains of E. coli O181, isolated from human cases of diarrhea, have been classified as belonging to this pathotype.
25861570	7	4	theme	anomeric	1453:1460	arg1	resonances					1469:1478	the five anomeric proton resonances	1444:1478	the five anomeric proton resonances	1444:1478	Both the OS and PSs exhibit rare conformational behavior since two of the five anomeric proton resonances could only be observed at an elevated temperature.
25861570	5	5	theme	chemical	832:839	arg1	shift					841:845	(13)C NMR chemical shift	822:845	(13)C NMR chemical shift	822:845	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	6	6	dep	determined	1082:1091	arg1	→4					1212:1213	→4	1212:1213	→4	1212:1213	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	6	6	dep	determined	1082:1091	arg1	-β-d-GlcpNAc-					1290:1302	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	6	7	dep	-β-d-GlcpNAc-	1290:1302	arg1	represent					1328:1336	represent	1328:1336	represent 0.4 equivalents per repeating unit	1328:1371	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	5	8	theme	OS	866:867	arg1	assignments					847:857	The (1)H and (13)C NMR chemical shift assignments	809:857	The (1)H and (13)C NMR chemical shift assignments of the OS	809:867	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	4	9	theme	48 	689:691	arg1	%					692:692	%	692:692	%	692:692	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	6	10	theme	-α-d-GalpNAc6Ac-	1234:1249	arg1	-β-d-GlcpNAc-					1290:1302	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	1	11	theme	diarrhea	254:261	arg1	cases					245:249	human cases	239:249	human cases of diarrhea	239:261	Shiga-toxin-producing Escherichia coli (STEC) is an important pathogen associated to food-borne infection in humans; strains of E. coli O181, isolated from human cases of diarrhea, have been classified as belonging to this pathotype.
25861570	6	12	theme	repeating	1161:1169	arg1	units					1171:1175	branched pentasaccharide repeating units	1136:1175	branched pentasaccharide repeating units joined by phosphodiester linkages	1136:1209	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	5	13	dep	spectrometry	981:992	arg1	data					1002:1005	data	1002:1005	data	1002:1005	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	5	13	dep	spectrometry	981:992	arg1	shifts					1039:1044	C NMR glycosylation shifts	1019:1044	C NMR glycosylation shifts	1019:1044	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	6	14	theme	native	1068:1073	arg1	PS					1075:1076	the native PS	1064:1076	the native PS	1064:1076	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	4	15	theme	aqueous	681:687	arg1	acid					707:710	aqueous 48 % hydrofluoric acid	681:710	aqueous 48 % hydrofluoric acid	681:710	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	6	16	theme	pentasaccharide	1145:1159	arg1	units					1171:1175	branched pentasaccharide repeating units	1136:1175	branched pentasaccharide repeating units joined by phosphodiester linkages	1136:1209	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	4	17	theme	hydrofluoric	694:705	arg1	acid					707:710	aqueous 48 % hydrofluoric acid	681:710	aqueous 48 % hydrofluoric acid	681:710	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	4	18	theme	oligosaccharide	599:613	arg1	spectrum					583:590	the high-resolution mass spectrum	558:590	the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid,	558:711	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	6	19	theme	branched	1136:1143	arg1	units					1171:1175	branched pentasaccharide repeating units	1136:1175	branched pentasaccharide repeating units joined by phosphodiester linkages	1136:1209	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	6	20	theme	[α-l-QuipNAc-	1215:1227	arg1	-β-d-GlcpNAc-					1290:1302	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	4	21	theme	%	692:692	arg1	acid					707:710	aqueous 48 % hydrofluoric acid	681:710	aqueous 48 % hydrofluoric acid	681:710	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	4	22	theme	spectrum	583:590	arg1	Analysis					546:553	Analysis	546:553	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid,	546:711	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	2	23	from	structure	329:337	arg1	E. coli					381:387	E. coli	381:387	E. coli	381:387	Herein, the structure of the O-antigen polysaccharide (PS) from E. coli O181 has been investigated.
25861570	4	24	theme	O-deacetylated	658:671	arg1	PS					673:674	the O-deacetylated PS	654:674	the O-deacetylated PS with aqueous 48 % hydrofluoric acid	654:710	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	4	25	theme	mass	578:581	arg1	spectrum					583:590	the high-resolution mass spectrum	558:590	the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid,	558:711	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	0	26	theme	Structural	0:9	arg1	Elucidation					11:21	Structural Elucidation	0:21	Structural Elucidation of the O-Antigen Polysaccharide from Escherichia coli O181	0:80	Structural Elucidation of the O-Antigen Polysaccharide from Escherichia coli O181.
25861570	5	27	theme	2 D	900:902	arg1	experiments					908:918	2 D NMR experiments	900:918	2 D NMR experiments	900:918	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	5	28	theme	H	816:816	arg1	assignments					847:857	The (1)H and (13)C NMR chemical shift assignments	809:857	The (1)H and (13)C NMR chemical shift assignments of the OS	809:867	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	2	29	theme	polysaccharide	356:369	arg1	structure					329:337	the structure	325:337	the structure of the O-antigen polysaccharide (PS) from E. coli O181	325:392	Herein, the structure of the O-antigen polysaccharide (PS) from E. coli O181 has been investigated.
25861570	6	30	theme	1→3	1229:1231	arg1	-β-d-GlcpNAc-					1290:1302	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	7	31	theme	elevated	1509:1516	arg1	temperature					1518:1528	an elevated temperature	1506:1528	an elevated temperature	1506:1528	Both the OS and PSs exhibit rare conformational behavior since two of the five anomeric proton resonances could only be observed at an elevated temperature.
25861570	5	32	dep	H	816:816	arg1	1					814:814	1	814:814	1	814:814	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	1	33	attach	isolated	225:232	arg2	strains					200:206	strains	200:206	strains	200:206	Shiga-toxin-producing Escherichia coli (STEC) is an important pathogen associated to food-borne infection in humans; strains of E. coli O181, isolated from human cases of diarrhea, have been classified as belonging to this pathotype.
25861570	1	33	attach	isolated	225:232	arg1	cases					245:249	human cases	239:249	human cases of diarrhea	239:261	Shiga-toxin-producing Escherichia coli (STEC) is an important pathogen associated to food-borne infection in humans; strains of E. coli O181, isolated from human cases of diarrhea, have been classified as belonging to this pathotype.
25861570	5	34	theme	C NMR	826:830	arg1	shift					841:845	(13)C NMR chemical shift	822:845	(13)C NMR chemical shift	822:845	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	5	35	theme	NMR	904:906	arg1	experiments					908:918	2 D NMR experiments	900:918	2 D NMR experiments	900:918	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	4	36	theme	PS	673:674	arg1	dephosphorylation					633:649	dephosphorylation	633:649	dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid	633:710	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	7	37	theme	rare	1402:1405	arg1	behavior					1422:1429	rare conformational behavior	1402:1429	rare conformational behavior	1402:1429	Both the OS and PSs exhibit rare conformational behavior since two of the five anomeric proton resonances could only be observed at an elevated temperature.
25861570	0	38	theme	Polysaccharide	40:53	arg1	Elucidation					11:21	Structural Elucidation	0:21	Structural Elucidation of the O-Antigen Polysaccharide from Escherichia coli O181	0:80	Structural Elucidation of the O-Antigen Polysaccharide from Escherichia coli O181.
25861570	1	39	theme	E. coli	211:217	arg1	O181					219:222	E. coli O181	211:222	E. coli O181	211:222	Shiga-toxin-producing Escherichia coli (STEC) is an important pathogen associated to food-borne infection in humans; strains of E. coli O181, isolated from human cases of diarrhea, have been classified as belonging to this pathotype.
25861570	0	40	dep	Escherichia	60:70	arg1	coli					72:75	coli	72:75	coli	72:75	Structural Elucidation of the O-Antigen Polysaccharide from Escherichia coli O181.
25861570	6	41	theme	-α-l-QuipNAc-	1272:1284	arg1	-β-d-GlcpNAc-					1290:1302	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	3	42	theme	sugar	421:425	arg1	analysis					427:434	The sugar analysis	417:434	The sugar analysis	417:434	The sugar analysis showed quinovosamine (QuiN), glucosamine (GlcN), galactosamine (GalN), and glucose (Glc) as major components.
25861570	0	43	theme	O-Antigen	30:38	arg1	Polysaccharide					40:53	the O-Antigen Polysaccharide	26:53	the O-Antigen Polysaccharide from Escherichia coli O181	26:80	Structural Elucidation of the O-Antigen Polysaccharide from Escherichia coli O181.
25861570	5	44	theme	tandem	969:974	arg1	spectrometry					981:992	tandem mass spectrometry	969:992	tandem mass spectrometry (MS/MS)	969:1000	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	5	44	theme	tandem	969:974	arg1	MS/MS					995:999	MS/MS	995:999	MS/MS	995:999	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	7	45	theme	conformational	1407:1420	arg1	behavior					1422:1429	rare conformational behavior	1402:1429	rare conformational behavior	1402:1429	Both the OS and PSs exhibit rare conformational behavior since two of the five anomeric proton resonances could only be observed at an elevated temperature.
25861570	0	46	from	O181	77:80	arg1	Polysaccharide					40:53	the O-Antigen Polysaccharide	26:53	the O-Antigen Polysaccharide from Escherichia coli O181	26:80	Structural Elucidation of the O-Antigen Polysaccharide from Escherichia coli O181.
25861570	0	46	from	O181	77:80	arg1	Elucidation					11:21	Structural Elucidation	0:21	Structural Elucidation of the O-Antigen Polysaccharide from Escherichia coli O181	0:80	Structural Elucidation of the O-Antigen Polysaccharide from Escherichia coli O181.
25861570	6	47	theme	repeating	1358:1366	arg1	unit					1368:1371	repeating unit	1358:1371	repeating unit	1358:1371	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	1	48	theme	O181	219:222	arg1	strains					200:206	strains	200:206	strains	200:206	Shiga-toxin-producing Escherichia coli (STEC) is an important pathogen associated to food-borne infection in humans; strains of E. coli O181, isolated from human cases of diarrhea, have been classified as belonging to this pathotype.
25861570	0	49	theme	Escherichia	60:70	arg1	O181					77:80	Escherichia coli O181	60:80	Escherichia coli O181	60:80	Structural Elucidation of the O-Antigen Polysaccharide from Escherichia coli O181.
25861570	4	50	with	PS	673:674	arg1	acid					707:710	aqueous 48 % hydrofluoric acid	681:710	aqueous 48 % hydrofluoric acid	681:710	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	5	51	theme	spectrometry	981:992	arg1	combination					954:964	a combination	952:964	a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts	952:1044	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	7	52	dep	OS	1383:1384	arg1	the					1379:1381	the	1379:1381	the	1379:1381	Both the OS and PSs exhibit rare conformational behavior since two of the five anomeric proton resonances could only be observed at an elevated temperature.
25861570	7	53	located	observed	1494:1501	arg1	temperature					1518:1528	an elevated temperature	1506:1528	an elevated temperature	1506:1528	Both the OS and PSs exhibit rare conformational behavior since two of the five anomeric proton resonances could only be observed at an elevated temperature.
25861570	7	53	located	observed	1494:1501	arg2	two					1437:1439	two	1437:1439	two	1437:1439	Both the OS and PSs exhibit rare conformational behavior since two of the five anomeric proton resonances could only be observed at an elevated temperature.
25861570	7	53	located	observed	1494:1501	arg2	resonances					1469:1478	the five anomeric proton resonances	1444:1478	the five anomeric proton resonances	1444:1478	Both the OS and PSs exhibit rare conformational behavior since two of the five anomeric proton resonances could only be observed at an elevated temperature.
25861570	6	54	theme	PS	1075:1076	arg1	structure					1051:1059	The structure	1047:1059	The structure of the native PS	1047:1076	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	6	55	theme	NMR	1099:1101	arg1	spectroscopy					1103:1114	NMR spectroscopy	1099:1114	NMR spectroscopy	1099:1114	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	5	56	theme	shift	841:845	arg1	assignments					847:857	The (1)H and (13)C NMR chemical shift assignments	809:857	The (1)H and (13)C NMR chemical shift assignments of the OS	809:867	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	6	57	theme	O-acetyl	1312:1319	arg1	groups					1321:1326	the O-acetyl groups	1308:1326	the O-acetyl groups	1308:1326	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	5	58	theme	C NMR	1019:1023	arg1	shifts					1039:1044	C NMR glycosylation shifts	1019:1044	C NMR glycosylation shifts	1019:1044	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	4	59	theme	Glc	796:798	arg1	QuiNAc					756:761	two QuiNAc	752:761	two QuiNAc	752:761	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	4	59	theme	Glc	796:798	arg1	residue					800:806	one Glc residue	792:806	one Glc residue	792:806	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	5	60	theme	13	823:824	arg1	C NMR					826:830	C NMR	826:830	C NMR	826:830	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	2	61	from	E. coli	381:387	arg1	PS					372:373	PS	372:373	PS	372:373	Herein, the structure of the O-antigen polysaccharide (PS) from E. coli O181 has been investigated.
25861570	2	61	from	E. coli	381:387	arg1	polysaccharide					356:369	the O-antigen polysaccharide	342:369	the O-antigen polysaccharide (PS) from E. coli O181	342:392	Herein, the structure of the O-antigen polysaccharide (PS) from E. coli O181 has been investigated.
25861570	2	61	from	E. coli	381:387	arg1	structure					329:337	the structure	325:337	the structure of the O-antigen polysaccharide (PS) from E. coli O181	325:392	Herein, the structure of the O-antigen polysaccharide (PS) from E. coli O181 has been investigated.
25861570	1	62	theme	important	135:143	arg1	coli					117:120	Escherichia coli	105:120	Shiga-toxin-producing Escherichia coli (STEC)	83:127	Shiga-toxin-producing Escherichia coli (STEC) is an important pathogen associated to food-borne infection in humans; strains of E. coli O181, isolated from human cases of diarrhea, have been classified as belonging to this pathotype.
25861570	1	62	theme	important	135:143	arg1	pathogen					145:152	an important pathogen	132:152	an important pathogen associated to food-borne infection in humans	132:197	Shiga-toxin-producing Escherichia coli (STEC) is an important pathogen associated to food-borne infection in humans; strains of E. coli O181, isolated from human cases of diarrhea, have been classified as belonging to this pathotype.
25861570	5	63	theme	glycosylation	1025:1037	arg1	shifts					1039:1044	C NMR glycosylation shifts	1019:1044	C NMR glycosylation shifts	1019:1044	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	4	64	theme	high-resolution	562:576	arg1	spectrum					583:590	the high-resolution mass spectrum	558:590	the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid,	558:711	Analysis of the high-resolution mass spectrum of the oligosaccharide (OS), obtained by dephosphorylation of the O-deacetylated PS with aqueous 48 % hydrofluoric acid, revealed a pentasaccharide composed of two QuiNAc, one GlcNAc, one GalNAc, and one Glc residue.
25861570	3	65	theme	major	528:532	arg1	components					534:543	major components	528:543	major components	528:543	The sugar analysis showed quinovosamine (QuiN), glucosamine (GlcN), galactosamine (GalN), and glucose (Glc) as major components.
25861570	3	65	theme	major	528:532	arg1	glucose					511:517	glucose	511:517	glucose (Glc)	511:523	The sugar analysis showed quinovosamine (QuiN), glucosamine (GlcN), galactosamine (GalN), and glucose (Glc) as major components.
25861570	3	65	theme	major	528:532	arg1	galactosamine					485:497	galactosamine	485:497	galactosamine (GalN)	485:504	The sugar analysis showed quinovosamine (QuiN), glucosamine (GlcN), galactosamine (GalN), and glucose (Glc) as major components.
25861570	3	65	theme	major	528:532	arg1	glucosamine					465:475	glucosamine	465:475	glucosamine (GlcN)	465:482	The sugar analysis showed quinovosamine (QuiN), glucosamine (GlcN), galactosamine (GalN), and glucose (Glc) as major components.
25861570	3	65	theme	major	528:532	arg1	quinovosamine					443:455	quinovosamine	443:455	quinovosamine (QuiN)	443:462	The sugar analysis showed quinovosamine (QuiN), glucosamine (GlcN), galactosamine (GalN), and glucose (Glc) as major components.
25861570	2	66	theme	O-antigen	346:354	arg1	PS					372:373	PS	372:373	PS	372:373	Herein, the structure of the O-antigen polysaccharide (PS) from E. coli O181 has been investigated.
25861570	2	66	theme	O-antigen	346:354	arg1	polysaccharide					356:369	the O-antigen polysaccharide	342:369	the O-antigen polysaccharide (PS) from E. coli O181	342:392	Herein, the structure of the O-antigen polysaccharide (PS) from E. coli O181 has been investigated.
25861570	6	67	theme	-α-d-Glcp-	1255:1264	arg1	-β-d-GlcpNAc-					1290:1302	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.	1215:1372	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	6	68	theme	phosphodiester	1187:1200	arg1	linkages					1202:1209	phosphodiester linkages	1187:1209	phosphodiester linkages	1187:1209	The structure of the native PS was determined using NMR spectroscopy, and it consists of branched pentasaccharide repeating units joined by phosphodiester linkages: →4)[α-l-QuipNAc-(1→3)]-α-d-GalpNAc6Ac-(1→6)-α-d-Glcp-(1→P-4)-α-l-QuipNAc-(1→3)-β-d-GlcpNAc-(1→; the O-acetyl groups represent 0.4 equivalents per repeating unit.
25861570	5	69	theme	mass	976:979	arg1	spectrometry					981:992	tandem mass spectrometry	969:992	tandem mass spectrometry (MS/MS)	969:1000	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	5	69	theme	mass	976:979	arg1	MS/MS					995:999	MS/MS	995:999	MS/MS	995:999	The (1)H and (13)C NMR chemical shift assignments of the OS were carried out using 1 D and 2 D NMR experiments, and the OS was sequenced using a combination of tandem mass spectrometry (MS/MS) data and NMR (13)C NMR glycosylation shifts.
25861570	1	70	from	infection	179:187	arg1	humans					192:197	humans	192:197	humans	192:197	Shiga-toxin-producing Escherichia coli (STEC) is an important pathogen associated to food-borne infection in humans; strains of E. coli O181, isolated from human cases of diarrhea, have been classified as belonging to this pathotype.
25861570	7	71	theme	resonances	1469:1478	arg1	resonances					1469:1478	the five anomeric proton resonances	1444:1478	the five anomeric proton resonances	1444:1478	Both the OS and PSs exhibit rare conformational behavior since two of the five anomeric proton resonances could only be observed at an elevated temperature.
25861570	7	71	theme	resonances	1469:1478	arg1	two					1437:1439	two	1437:1439	two	1437:1439	Both the OS and PSs exhibit rare conformational behavior since two of the five anomeric proton resonances could only be observed at an elevated temperature.
25861570	1	72	theme	human	239:243	arg1	cases					245:249	human cases	239:249	human cases of diarrhea	239:261	Shiga-toxin-producing Escherichia coli (STEC) is an important pathogen associated to food-borne infection in humans; strains of E. coli O181, isolated from human cases of diarrhea, have been classified as belonging to this pathotype.
25031069	5	0	theme	quantitative	583:594	arg1	PCR					596:598	Real-time quantitative PCR	573:598	Real-time quantitative PCR	573:598	Real-time quantitative PCR was employed to determine the effects imposed to gene transcripts in the colon and lymph nodes.
25031069	7	1	theme	semisynthetic	776:788	arg1	diets					790:794	semisynthetic diets	776:794	semisynthetic diets free from FFs	776:808	RESULTS NOD mice fed semisynthetic diets free from FFs were largely protected from diabetes while semisynthetic diets supplemented with the FFs pectin and xylan (PX) resulted in higher diabetes incidence.
25031069	12	2	theme	NOD	1965:1967	arg1	mice					1969:1972	NOD mice	1965:1972	NOD mice	1965:1972	CONCLUSIONS/INTERPRETATION FFs condition microbiota, affect colon homeostasis and are important components of natural, diabetes-promoting diets in NOD mice.
25031069	5	3	theme	Real-time	573:581	arg1	PCR					596:598	Real-time quantitative PCR	573:598	Real-time quantitative PCR	573:598	Real-time quantitative PCR was employed to determine the effects imposed to gene transcripts in the colon and lymph nodes.
25031069	11	4	theme	FF-free	1804:1810	arg1	diet					1812:1815	diabetes-protective FF-free diet	1784:1815	diabetes-protective FF-free diet	1784:1815	16S RNA sequencing revealed each diet to give rise to its particular GM composition, with different Firmicutes to Bacteroidetes ratios, and enrichment of mucin-degrading Ruminococcaceae following diabetes-protective FF-free diet.
25031069	9	5	theme	semisynthetic	1348:1360	arg1	diet					1362:1365	the semisynthetic diet	1344:1365	the semisynthetic diet free from FFs	1344:1379	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	8	6	theme	GM	1077:1078	arg1	composition					1058:1068	the composition	1054:1068	the composition of the GM towards that found in natural-diet-fed mice	1054:1122	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	12	7	theme	CONCLUSIONS/INTERPRETATION	1818:1843	arg1	FFs					1845:1847	CONCLUSIONS/INTERPRETATION FFs	1818:1847	CONCLUSIONS/INTERPRETATION FFs	1818:1847	CONCLUSIONS/INTERPRETATION FFs condition microbiota, affect colon homeostasis and are important components of natural, diabetes-promoting diets in NOD mice.
25031069	9	8	theme	free	1367:1370	arg1	diet					1362:1365	the semisynthetic diet	1344:1365	the semisynthetic diet free from FFs	1344:1379	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	8	9	theme	short-chain	1163:1173	arg1	metabolites					1186:1196	FF-derived short-chain fatty acid metabolites	1152:1196	FF-derived short-chain fatty acid metabolites in the colon	1152:1209	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	11	10	theme	Bacteroidetes	1702:1714	arg1	ratios					1716:1721	Bacteroidetes ratios	1702:1721	Bacteroidetes ratios	1702:1721	16S RNA sequencing revealed each diet to give rise to its particular GM composition, with different Firmicutes to Bacteroidetes ratios, and enrichment of mucin-degrading Ruminococcaceae following diabetes-protective FF-free diet.
25031069	12	11	theme	diets	1956:1960	arg1	components					1914:1923	important components	1904:1923	important components of natural, diabetes-promoting diets in NOD mice	1904:1972	CONCLUSIONS/INTERPRETATION FFs condition microbiota, affect colon homeostasis and are important components of natural, diabetes-promoting diets in NOD mice.
25031069	12	11	theme	diets	1956:1960	arg1	condition					1849:1857	condition	1849:1857	condition	1849:1857	CONCLUSIONS/INTERPRETATION FFs condition microbiota, affect colon homeostasis and are important components of natural, diabetes-promoting diets in NOD mice.
25031069	8	12	theme	acid	1181:1184	arg1	metabolites					1186:1196	FF-derived short-chain fatty acid metabolites	1152:1196	FF-derived short-chain fatty acid metabolites in the colon	1152:1209	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	10	13	theme	same	1481:1484	arg1	diet					1486:1489	the same diet	1477:1489	the same diet	1477:1489	PX in the same diet counteracted these effects and promoted stress-related IL-18 activation in gut epithelial cells.
25031069	2	14	theme	ample	246:250	arg1	supply					252:257	an ample supply	243:257	an ample supply in natural, diabetes-promoting diets	243:294	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	1	15	theme	type	182:185	arg1	diabetes					189:196	type 1 diabetes	182:196	type 1 diabetes	182:196	AIMS/HYPOTHESIS Gut microbiota (GM) and diet both appear to be important in the pathogenesis of type 1 diabetes.
25031069	12	16	theme	important	1904:1912	arg1	components					1914:1923	important components	1904:1923	important components of natural, diabetes-promoting diets in NOD mice	1904:1972	CONCLUSIONS/INTERPRETATION FFs condition microbiota, affect colon homeostasis and are important components of natural, diabetes-promoting diets in NOD mice.
25031069	9	17	theme	Il22	1395:1398	arg1	transcripts					1416:1426	Il4, Il22, Tgfβ and Foxp3 transcripts	1390:1426	Il4, Il22, Tgfβ and Foxp3 transcripts	1390:1426	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	9	18	theme	Tgfβ	1401:1404	arg1	transcripts					1416:1426	Il4, Il22, Tgfβ and Foxp3 transcripts	1390:1426	Il4, Il22, Tgfβ and Foxp3 transcripts	1390:1426	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	2	19	dep	natural	262:268	arg1	diabetes-promoting					271:288	diabetes-promoting	271:288	diabetes-promoting	271:288	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	11	20	theme	particular	1646:1655	arg1	composition					1660:1670	its particular GM composition	1642:1670	its particular GM composition	1642:1670	16S RNA sequencing revealed each diet to give rise to its particular GM composition, with different Firmicutes to Bacteroidetes ratios, and enrichment of mucin-degrading Ruminococcaceae following diabetes-protective FF-free diet.
25031069	7	21	theme	higher	933:938	arg1	incidence					949:957	higher diabetes incidence	933:957	higher diabetes incidence	933:957	RESULTS NOD mice fed semisynthetic diets free from FFs were largely protected from diabetes while semisynthetic diets supplemented with the FFs pectin and xylan (PX) resulted in higher diabetes incidence.
25031069	9	22	theme	Foxp3	1410:1414	arg1	transcripts					1416:1426	Il4, Il22, Tgfβ and Foxp3 transcripts	1390:1426	Il4, Il22, Tgfβ and Foxp3 transcripts	1390:1426	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	1	23	dep	microbiota	106:115	arg1	both					131:134	both	131:134	both	131:134	AIMS/HYPOTHESIS Gut microbiota (GM) and diet both appear to be important in the pathogenesis of type 1 diabetes.
25031069	7	24	theme	FFs	895:897	arg1	pectin					899:904	the FFs pectin	891:904	the FFs pectin	891:904	RESULTS NOD mice fed semisynthetic diets free from FFs were largely protected from diabetes while semisynthetic diets supplemented with the FFs pectin and xylan (PX) resulted in higher diabetes incidence.
25031069	11	25	theme	mucin-degrading	1742:1756	arg1	Ruminococcaceae					1758:1772	mucin-degrading Ruminococcaceae	1742:1772	mucin-degrading Ruminococcaceae	1742:1772	16S RNA sequencing revealed each diet to give rise to its particular GM composition, with different Firmicutes to Bacteroidetes ratios, and enrichment of mucin-degrading Ruminococcaceae following diabetes-protective FF-free diet.
25031069	2	26	from	supply	252:257	arg1	diets					290:294	natural, diabetes-promoting diets	262:294	natural, diabetes-promoting diets	262:294	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	2	27	used	used	301:304	arg2	source					317:322	a source	315:322	a source of energy	315:332	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	2	27	used	used	301:304	arg2	FFs					219:221	FFs	219:221	FFs	219:221	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	2	27	used	used	301:304	arg2	fibres					211:216	Fermentable fibres	199:216	Fermentable fibres (FFs)	199:222	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	8	28	from	colon	1205:1209	arg1	production					1138:1147	production	1138:1147	production of FF-derived short-chain fatty acid metabolites in the colon	1138:1209	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	9	29	theme	pancreatic	1448:1457	arg1	node					1465:1468	pancreatic lymph node	1448:1468	pancreatic lymph node	1448:1468	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	10	30	theme	gut	1566:1568	arg1	cells					1581:1585	gut epithelial cells	1566:1585	gut epithelial cells	1566:1585	PX in the same diet counteracted these effects and promoted stress-related IL-18 activation in gut epithelial cells.
25031069	9	31	theme	natural	1236:1242	arg1	diet					1244:1247	The highly diabetogenic natural diet	1212:1247	The highly diabetogenic natural diet	1212:1247	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	9	32	from	expression	1269:1278	arg1	colon					1331:1335	the colon	1327:1335	the colon	1327:1335	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	7	33	from	FFs	806:808	arg1	free					796:799	free	796:799	free	796:799	RESULTS NOD mice fed semisynthetic diets free from FFs were largely protected from diabetes while semisynthetic diets supplemented with the FFs pectin and xylan (PX) resulted in higher diabetes incidence.
25031069	0	34	theme	Fermentable	0:10	arg1	fibres					12:17	Fermentable fibres	0:17	Fermentable fibres	0:17	Fermentable fibres condition colon microbiota and promote diabetogenesis in NOD mice.
25031069	1	35	theme	Gut	102:104	arg1	GM					118:119	GM	118:119	GM	118:119	AIMS/HYPOTHESIS Gut microbiota (GM) and diet both appear to be important in the pathogenesis of type 1 diabetes.
25031069	1	35	theme	Gut	102:104	arg1	microbiota					106:115	AIMS/HYPOTHESIS Gut microbiota	86:115	AIMS/HYPOTHESIS Gut microbiota (GM)	86:120	AIMS/HYPOTHESIS Gut microbiota (GM) and diet both appear to be important in the pathogenesis of type 1 diabetes.
25031069	1	36	from	pathogenesis	166:177	arg1	important					149:157	important	149:157	important	149:157	AIMS/HYPOTHESIS Gut microbiota (GM) and diet both appear to be important in the pathogenesis of type 1 diabetes.
25031069	4	37	dep	METHODS	423:429	arg1	evaluated					562:570	evaluated	562:570	were evaluated	557:570	METHODS Female NOD mice were weaned to a semisynthetic diet and the effects of FF supplementation on diabetes incidence and insulitis were evaluated.
25031069	4	37	dep	METHODS	423:429	arg1	weaned					452:457	weaned	452:457	were weaned to a semisynthetic diet	447:481	METHODS Female NOD mice were weaned to a semisynthetic diet and the effects of FF supplementation on diabetes incidence and insulitis were evaluated.
25031069	4	38	theme	Female	431:436	arg1	mice					442:445	Female NOD mice	431:445	Female NOD mice	431:445	METHODS Female NOD mice were weaned to a semisynthetic diet and the effects of FF supplementation on diabetes incidence and insulitis were evaluated.
25031069	8	39	located	found	1093:1097	arg1	mice					1119:1122	natural-diet-fed mice	1102:1122	natural-diet-fed mice	1102:1122	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	8	39	located	found	1093:1097	arg2	composition					1058:1068	the composition	1054:1068	the composition of the GM towards that found in natural-diet-fed mice	1054:1122	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	10	40	from	PX	1471:1472	arg1	diet					1486:1489	the same diet	1477:1489	the same diet	1477:1489	PX in the same diet counteracted these effects and promoted stress-related IL-18 activation in gut epithelial cells.
25031069	3	41	theme	diabetes	386:393	arg1	incidence					395:403	diabetes incidence	386:403	diabetes incidence	386:403	Our aim was to determine whether FFs modify GM and diabetes incidence in the NOD mouse.
25031069	1	42	from	important	149:157	arg1	pathogenesis					166:177	the pathogenesis	162:177	the pathogenesis of type 1 diabetes	162:196	AIMS/HYPOTHESIS Gut microbiota (GM) and diet both appear to be important in the pathogenesis of type 1 diabetes.
25031069	9	43	theme	genes	1318:1322	arg1	expression					1269:1278	expression	1269:1278	expression of proinflammatory and stress-related genes in the colon	1269:1335	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	6	44	theme	next-generation	727:741	arg1	sequencing					743:752	next-generation sequencing	727:752	next-generation sequencing	727:752	Changes to GM were analysed by next-generation sequencing.
25031069	2	45	theme	energy	327:332	arg1	source					317:322	a source	315:322	a source of energy	315:332	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	2	45	theme	energy	327:332	arg1	fibres					211:216	Fermentable fibres	199:216	Fermentable fibres (FFs)	199:222	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	4	46	theme	supplementation	505:519	arg1	effects					491:497	the effects	487:497	the effects of FF supplementation on diabetes incidence and insulitis	487:555	METHODS Female NOD mice were weaned to a semisynthetic diet and the effects of FF supplementation on diabetes incidence and insulitis were evaluated.
25031069	3	47	theme	NOD	412:414	arg1	mouse					416:420	the NOD mouse	408:420	the NOD mouse	408:420	Our aim was to determine whether FFs modify GM and diabetes incidence in the NOD mouse.
25031069	11	48	theme	16S	1588:1590	arg1	sequencing					1596:1605	16S RNA sequencing	1588:1605	16S RNA sequencing	1588:1605	16S RNA sequencing revealed each diet to give rise to its particular GM composition, with different Firmicutes to Bacteroidetes ratios, and enrichment of mucin-degrading Ruminococcaceae following diabetes-protective FF-free diet.
25031069	2	49	theme	natural	262:268	arg1	diets					290:294	natural, diabetes-promoting diets	262:294	natural, diabetes-promoting diets	262:294	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	7	50	theme	NOD	763:765	arg1	mice					767:770	NOD mice	763:770	NOD mice fed semisynthetic diets free from FFs	763:808	RESULTS NOD mice fed semisynthetic diets free from FFs were largely protected from diabetes while semisynthetic diets supplemented with the FFs pectin and xylan (PX) resulted in higher diabetes incidence.
25031069	12	51	theme	natural	1928:1934	arg1	diets					1956:1960	natural, diabetes-promoting diets	1928:1960	natural, diabetes-promoting diets	1928:1960	CONCLUSIONS/INTERPRETATION FFs condition microbiota, affect colon homeostasis and are important components of natural, diabetes-promoting diets in NOD mice.
25031069	8	52	theme	FF-derived	1152:1161	arg1	metabolites					1186:1196	FF-derived short-chain fatty acid metabolites	1152:1196	FF-derived short-chain fatty acid metabolites in the colon	1152:1209	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	9	53	theme	Il4	1390:1392	arg1	transcripts					1416:1426	Il4, Il22, Tgfβ and Foxp3 transcripts	1390:1426	Il4, Il22, Tgfβ and Foxp3 transcripts	1390:1426	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	4	54	theme	diabetes	524:531	arg1	incidence					533:541	diabetes incidence	524:541	diabetes incidence	524:541	METHODS Female NOD mice were weaned to a semisynthetic diet and the effects of FF supplementation on diabetes incidence and insulitis were evaluated.
25031069	10	55	theme	stress-related	1531:1544	arg1	activation					1552:1561	stress-related IL-18 activation	1531:1561	stress-related IL-18 activation	1531:1561	PX in the same diet counteracted these effects and promoted stress-related IL-18 activation in gut epithelial cells.
25031069	5	56	from	transcripts	654:664	arg1	nodes					689:693	the colon and lymph nodes	669:693	nodes	689:693	Real-time quantitative PCR was employed to determine the effects imposed to gene transcripts in the colon and lymph nodes.
25031069	8	57	theme	natural-diet-fed	1102:1117	arg1	mice					1119:1122	natural-diet-fed mice	1102:1122	natural-diet-fed mice	1102:1122	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	8	58	theme	metabolites	1186:1196	arg1	production					1138:1147	production	1138:1147	production of FF-derived short-chain fatty acid metabolites in the colon	1138:1209	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	7	59	theme	diabetes	940:947	arg1	incidence					949:957	higher diabetes incidence	933:957	higher diabetes incidence	933:957	RESULTS NOD mice fed semisynthetic diets free from FFs were largely protected from diabetes while semisynthetic diets supplemented with the FFs pectin and xylan (PX) resulted in higher diabetes incidence.
25031069	2	60	theme	Fermentable	199:209	arg1	fibres					211:216	Fermentable fibres	199:216	Fermentable fibres (FFs)	199:222	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	2	60	theme	Fermentable	199:209	arg1	source					317:322	a source	315:322	a source of energy	315:332	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	2	60	theme	Fermentable	199:209	arg1	FFs					219:221	FFs	219:221	FFs	219:221	Fermentable fibres (FFs), of which there is an ample supply in natural, diabetes-promoting diets, are used by GM as a source of energy.
25031069	8	61	from	production	1138:1147	arg1	colon					1205:1209	the colon	1201:1209	the colon	1201:1209	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	11	62	theme	GM	1657:1658	arg1	composition					1660:1670	its particular GM composition	1642:1670	its particular GM composition	1642:1670	16S RNA sequencing revealed each diet to give rise to its particular GM composition, with different Firmicutes to Bacteroidetes ratios, and enrichment of mucin-degrading Ruminococcaceae following diabetes-protective FF-free diet.
25031069	0	63	theme	NOD	76:78	arg1	mice					80:83	NOD mice	76:83	NOD mice	76:83	Fermentable fibres condition colon microbiota and promote diabetogenesis in NOD mice.
25031069	8	64	from	metabolites	1186:1196	arg1	colon					1205:1209	the colon	1201:1209	the colon	1201:1209	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	8	65	from	FFs	989:991	arg1	free					979:982	free	979:982	free	979:982	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	5	66	theme	colon	673:677	arg1	nodes					689:693	the colon and lymph nodes	669:693	nodes	689:693	Real-time quantitative PCR was employed to determine the effects imposed to gene transcripts in the colon and lymph nodes.
25031069	12	67	theme	colon	1878:1882	arg1	homeostasis					1884:1894	colon homeostasis	1878:1894	colon homeostasis	1878:1894	CONCLUSIONS/INTERPRETATION FFs condition microbiota, affect colon homeostasis and are important components of natural, diabetes-promoting diets in NOD mice.
25031069	11	68	theme	different	1678:1686	arg1	Firmicutes					1688:1697	different Firmicutes	1678:1697	different Firmicutes to Bacteroidetes ratios	1678:1721	16S RNA sequencing revealed each diet to give rise to its particular GM composition, with different Firmicutes to Bacteroidetes ratios, and enrichment of mucin-degrading Ruminococcaceae following diabetes-protective FF-free diet.
25031069	4	69	from	effects	491:497	arg1	insulitis					547:555	insulitis	547:555	insulitis	547:555	METHODS Female NOD mice were weaned to a semisynthetic diet and the effects of FF supplementation on diabetes incidence and insulitis were evaluated.
25031069	4	69	from	effects	491:497	arg1	incidence					533:541	diabetes incidence	524:541	diabetes incidence	524:541	METHODS Female NOD mice were weaned to a semisynthetic diet and the effects of FF supplementation on diabetes incidence and insulitis were evaluated.
25031069	12	70	from	condition	1849:1857	arg1	mice					1969:1972	NOD mice	1965:1972	NOD mice	1965:1972	CONCLUSIONS/INTERPRETATION FFs condition microbiota, affect colon homeostasis and are important components of natural, diabetes-promoting diets in NOD mice.
25031069	0	71	from	diabetogenesis	58:71	arg1	mice					80:83	NOD mice	76:83	NOD mice	76:83	Fermentable fibres condition colon microbiota and promote diabetogenesis in NOD mice.
25031069	5	72	theme	gene	649:652	arg1	transcripts					654:664	gene transcripts	649:664	gene transcripts in the colon and lymph nodes	649:693	Real-time quantitative PCR was employed to determine the effects imposed to gene transcripts in the colon and lymph nodes.
25031069	9	73	theme	diabetogenic	1223:1234	arg1	diet					1244:1247	The highly diabetogenic natural diet	1212:1247	The highly diabetogenic natural diet	1212:1247	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	8	74	theme	Semisynthetic	960:972	arg1	diet					974:977	Semisynthetic diet	960:977	Semisynthetic diet free from FFs	960:991	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	8	75	theme	fatty	1175:1179	arg1	metabolites					1186:1196	FF-derived short-chain fatty acid metabolites	1152:1196	FF-derived short-chain fatty acid metabolites in the colon	1152:1209	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	9	76	theme	lymph	1459:1463	arg1	node					1465:1468	pancreatic lymph node	1448:1468	pancreatic lymph node	1448:1468	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	8	77	theme	free	979:982	arg1	diet					974:977	Semisynthetic diet	960:977	Semisynthetic diet free from FFs	960:991	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	7	78	theme	semisynthetic	853:865	arg1	diets					867:871	semisynthetic diets	853:871	semisynthetic diets supplemented with the FFs pectin and xylan (PX)	853:919	RESULTS NOD mice fed semisynthetic diets free from FFs were largely protected from diabetes while semisynthetic diets supplemented with the FFs pectin and xylan (PX) resulted in higher diabetes incidence.
25031069	1	79	theme	AIMS/HYPOTHESIS	86:100	arg1	GM					118:119	GM	118:119	GM	118:119	AIMS/HYPOTHESIS Gut microbiota (GM) and diet both appear to be important in the pathogenesis of type 1 diabetes.
25031069	1	79	theme	AIMS/HYPOTHESIS	86:100	arg1	microbiota					106:115	AIMS/HYPOTHESIS Gut microbiota	86:115	AIMS/HYPOTHESIS Gut microbiota (GM)	86:120	AIMS/HYPOTHESIS Gut microbiota (GM) and diet both appear to be important in the pathogenesis of type 1 diabetes.
25031069	12	80	dep	natural	1928:1934	arg1	diabetes-promoting					1937:1954	diabetes-promoting	1937:1954	diabetes-promoting	1937:1954	CONCLUSIONS/INTERPRETATION FFs condition microbiota, affect colon homeostasis and are important components of natural, diabetes-promoting diets in NOD mice.
25031069	10	81	theme	IL-18	1546:1550	arg1	activation					1552:1561	stress-related IL-18 activation	1531:1561	stress-related IL-18 activation	1531:1561	PX in the same diet counteracted these effects and promoted stress-related IL-18 activation in gut epithelial cells.
25031069	0	82	theme	colon	29:33	arg1	microbiota					35:44	colon microbiota	29:44	colon microbiota	29:44	Fermentable fibres condition colon microbiota and promote diabetogenesis in NOD mice.
25031069	4	83	theme	semisynthetic	464:476	arg1	diet					478:481	a semisynthetic diet	462:481	a semisynthetic diet	462:481	METHODS Female NOD mice were weaned to a semisynthetic diet and the effects of FF supplementation on diabetes incidence and insulitis were evaluated.
25031069	5	84	theme	lymph	683:687	arg1	nodes					689:693	the colon and lymph nodes	669:693	nodes	689:693	Real-time quantitative PCR was employed to determine the effects imposed to gene transcripts in the colon and lymph nodes.
25031069	8	85	theme	GM	1001:1002	arg1	composition					1004:1014	GM composition	1001:1014	GM composition	1001:1014	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	8	86	link	FF-derived	1152:1161	arg1	metabolites					1186:1196	FF-derived short-chain fatty acid metabolites	1152:1196	FF-derived short-chain fatty acid metabolites in the colon	1152:1209	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	4	87	theme	NOD	438:440	arg1	mice					442:445	Female NOD mice	431:445	Female NOD mice	431:445	METHODS Female NOD mice were weaned to a semisynthetic diet and the effects of FF supplementation on diabetes incidence and insulitis were evaluated.
25031069	11	88	theme	RNA	1592:1594	arg1	sequencing					1596:1605	16S RNA sequencing	1588:1605	16S RNA sequencing	1588:1605	16S RNA sequencing revealed each diet to give rise to its particular GM composition, with different Firmicutes to Bacteroidetes ratios, and enrichment of mucin-degrading Ruminococcaceae following diabetes-protective FF-free diet.
25031069	7	89	dep	RESULTS	755:761	arg1	protected					823:831	protected	823:831	were largely protected from diabetes while semisynthetic diets supplemented with the FFs pectin and xylan (PX) resulted in higher diabetes incidence	810:957	RESULTS NOD mice fed semisynthetic diets free from FFs were largely protected from diabetes while semisynthetic diets supplemented with the FFs pectin and xylan (PX) resulted in higher diabetes incidence.
25031069	10	90	theme	epithelial	1570:1579	arg1	cells					1581:1585	gut epithelial cells	1566:1585	gut epithelial cells	1566:1585	PX in the same diet counteracted these effects and promoted stress-related IL-18 activation in gut epithelial cells.
25031069	9	91	theme	proinflammatory	1283:1297	arg1	genes					1318:1322	proinflammatory and stress-related genes	1283:1322	genes	1318:1322	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	11	92	theme	diabetes-protective	1784:1802	arg1	diet					1812:1815	diabetes-protective FF-free diet	1784:1815	diabetes-protective FF-free diet	1784:1815	16S RNA sequencing revealed each diet to give rise to its particular GM composition, with different Firmicutes to Bacteroidetes ratios, and enrichment of mucin-degrading Ruminococcaceae following diabetes-protective FF-free diet.
25031069	9	93	theme	stress-related	1303:1316	arg1	genes					1318:1322	proinflammatory and stress-related genes	1283:1322	genes	1318:1322	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	12	94	from	components	1914:1923	arg1	mice					1969:1972	NOD mice	1965:1972	NOD mice	1965:1972	CONCLUSIONS/INTERPRETATION FFs condition microbiota, affect colon homeostasis and are important components of natural, diabetes-promoting diets in NOD mice.
25031069	4	95	theme	FF	502:503	arg1	supplementation					505:519	FF supplementation	502:519	FF supplementation	502:519	METHODS Female NOD mice were weaned to a semisynthetic diet and the effects of FF supplementation on diabetes incidence and insulitis were evaluated.
25031069	11	96	theme	Ruminococcaceae	1758:1772	arg1	enrichment					1728:1737	enrichment	1728:1737	enrichment of mucin-degrading Ruminococcaceae	1728:1772	16S RNA sequencing revealed each diet to give rise to its particular GM composition, with different Firmicutes to Bacteroidetes ratios, and enrichment of mucin-degrading Ruminococcaceae following diabetes-protective FF-free diet.
25031069	8	97	theme	PX	1043:1044	arg1	addition					1031:1038	addition	1031:1038	addition of PX	1031:1044	Semisynthetic diet free from FFs altered GM composition significantly; addition of PX changed the composition of the GM towards that found in natural-diet-fed mice and increased production of FF-derived short-chain fatty acid metabolites in the colon.
25031069	1	98	theme	diabetes	189:196	arg1	pathogenesis					166:177	the pathogenesis	162:177	the pathogenesis of type 1 diabetes	162:196	AIMS/HYPOTHESIS Gut microbiota (GM) and diet both appear to be important in the pathogenesis of type 1 diabetes.
25031069	9	99	from	FFs	1377:1379	arg1	free					1367:1370	free	1367:1370	free	1367:1370	The highly diabetogenic natural diet was associated with expression of proinflammatory and stress-related genes in the colon, while the semisynthetic diet free from FFs promoted Il4, Il22, Tgfβ and Foxp3 transcripts in the colon and/or pancreatic lymph node.
25031069	7	100	theme	free	796:799	arg1	diets					790:794	semisynthetic diets	776:794	semisynthetic diets free from FFs	776:808	RESULTS NOD mice fed semisynthetic diets free from FFs were largely protected from diabetes while semisynthetic diets supplemented with the FFs pectin and xylan (PX) resulted in higher diabetes incidence.
25452500	6	0	theme	carbohydrate	1025:1036	arg1	–					1048:1048	the primary milk carbohydrate – lactose –	1008:1048	the primary milk carbohydrate – lactose –	1008:1048	However, the concentration of the primary milk carbohydrate – lactose – was higher.
25452500	1	1	theme	selection	214:222	arg1	rate					142:145	the high basal metabolic rate	117:145	the high basal metabolic rate (BMR) of homeotherms	117:166	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	1	1	theme	selection	214:222	arg1	by-product					192:201	a by-product	190:201	a by-product of natural selection for effective parental care	190:250	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	0	2	theme	metabolic	67:75	arg1	rate					77:80	basal metabolic rate	61:80	basal metabolic rate	61:80	Milk output and composition in mice divergently selected for basal metabolic rate.
25452500	0	3	from	output	5:10	arg1	mice					31:34	mice	31:34	mice	31:34	Milk output and composition in mice divergently selected for basal metabolic rate.
25452500	4	4	theme	h	817:817	arg1	period					819:824	a 24 h period	812:824	a 24 h period in both reproductive bouts	812:851	We found that the mice selected for high BMR produced significantly more milk in a 24 h period in both reproductive bouts.
25452500	6	5	theme	milk	1020:1023	arg1	–					1048:1048	the primary milk carbohydrate – lactose –	1008:1048	the primary milk carbohydrate – lactose –	1008:1048	However, the concentration of the primary milk carbohydrate – lactose – was higher.
25452500	3	6	theme	17°C	660:663	arg1	temperature					645:655	an ambient temperature	634:655	an ambient temperature of 17°C	634:663	Additionally, to remedy the potential limitation imposed by the ability of mother mice to dissipate excess heat, we exposed them to an ambient temperature of 17°C during the most energetically demanding second week of lactation.
25452500	5	7	contain	had	906:908	arg2	concentration					924:936	lower protein concentration	910:936	lower protein concentration	910:936	The milk samples obtained from the high BMR females had lower protein concentration and did not differ with respect to fat.
25452500	5	7	contain	had	906:908	arg1	samples					863:869	The milk samples	854:869	The milk samples obtained from the high BMR females	854:904	The milk samples obtained from the high BMR females had lower protein concentration and did not differ with respect to fat.
25452500	1	8	theme	evolutionary	91:102	arg1	perspective					104:114	an evolutionary perspective	88:114	an evolutionary perspective	88:114	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	1	9	theme	effective	228:236	arg1	care					247:250	effective parental care	228:250	effective parental care	228:250	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	3	10	theme	demanding	695:703	arg1	week					712:715	the most energetically demanding second week	672:715	the most energetically demanding second week of lactation	672:728	Additionally, to remedy the potential limitation imposed by the ability of mother mice to dissipate excess heat, we exposed them to an ambient temperature of 17°C during the most energetically demanding second week of lactation.
25452500	5	11	theme	lower	910:914	arg1	concentration					924:936	lower protein concentration	910:936	lower protein concentration	910:936	The milk samples obtained from the high BMR females had lower protein concentration and did not differ with respect to fat.
25452500	2	12	from	bouts	317:321	arg1	mice					326:329	mice	326:329	mice divergently selected for high/low BMR	326:367	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	3	13	theme	potential	530:538	arg1	limitation					540:549	the potential limitation	526:549	the potential limitation imposed by the ability of mother mice to dissipate excess heat	526:612	Additionally, to remedy the potential limitation imposed by the ability of mother mice to dissipate excess heat, we exposed them to an ambient temperature of 17°C during the most energetically demanding second week of lactation.
25452500	1	14	theme	parental	238:245	arg1	care					247:250	effective parental care	228:250	effective parental care	228:250	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	2	15	from	differences	430:440	arg1	abilities					471:479	the between-line suckling abilities	445:479	the between-line suckling abilities of nursed juveniles	445:499	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	5	16	theme	protein	916:922	arg1	concentration					924:936	lower protein concentration	910:936	lower protein concentration	910:936	The milk samples obtained from the high BMR females had lower protein concentration and did not differ with respect to fat.
25452500	7	17	theme	physiological	1262:1274	arg1	traits					1276:1281	the physiological traits	1258:1281	the physiological traits underlying BMR and lactation performance	1258:1322	Although all the above between-line differences were statistically significant, their magnitude was too small to unambiguously ascribe them as stemming from a positive genetic correlation between the physiological traits underlying BMR and lactation performance.
25452500	6	18	theme	primary	1012:1018	arg1	–					1048:1048	the primary milk carbohydrate – lactose –	1008:1048	the primary milk carbohydrate – lactose –	1008:1048	However, the concentration of the primary milk carbohydrate – lactose – was higher.
25452500	2	19	theme	juveniles	491:499	arg1	abilities					471:479	the between-line suckling abilities	445:479	the between-line suckling abilities of nursed juveniles	445:499	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	7	20	theme	BMR	1294:1296	arg1	performance					1312:1322	BMR and lactation performance	1294:1322	BMR and lactation performance	1294:1322	Although all the above between-line differences were statistically significant, their magnitude was too small to unambiguously ascribe them as stemming from a positive genetic correlation between the physiological traits underlying BMR and lactation performance.
25452500	2	21	theme	potential	420:428	arg1	differences					430:440	potential differences	420:440	potential differences in the between-line suckling abilities of nursed juveniles	420:499	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	2	22	theme	suckling	462:469	arg1	abilities					471:479	the between-line suckling abilities	445:479	the between-line suckling abilities of nursed juveniles	445:499	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	4	23	theme	24	814:815	arg1	h					817:817	h	817:817	h	817:817	We found that the mice selected for high BMR produced significantly more milk in a 24 h period in both reproductive bouts.
25452500	2	24	theme	nursed	484:489	arg1	juveniles					491:499	nursed juveniles	484:499	nursed juveniles	484:499	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	3	25	theme	second	705:710	arg1	week					712:715	the most energetically demanding second week	672:715	the most energetically demanding second week of lactation	672:728	Additionally, to remedy the potential limitation imposed by the ability of mother mice to dissipate excess heat, we exposed them to an ambient temperature of 17°C during the most energetically demanding second week of lactation.
25452500	2	26	theme	high/low	356:363	arg1	BMR					365:367	high/low BMR	356:367	high/low BMR	356:367	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	0	27	theme	Milk	0:3	arg1	output					5:10	Milk output	0:10	Milk output	0:10	Milk output and composition in mice divergently selected for basal metabolic rate.
25452500	3	28	theme	lactation	720:728	arg1	week					712:715	the most energetically demanding second week	672:715	the most energetically demanding second week of lactation	672:728	Additionally, to remedy the potential limitation imposed by the ability of mother mice to dissipate excess heat, we exposed them to an ambient temperature of 17°C during the most energetically demanding second week of lactation.
25452500	5	29	theme	high	889:892	arg1	females					898:904	the high BMR females	885:904	the high BMR females	885:904	The milk samples obtained from the high BMR females had lower protein concentration and did not differ with respect to fat.
25452500	1	30	theme	homeotherms	156:166	arg1	BMR					148:150	BMR	148:150	BMR	148:150	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	1	30	theme	homeotherms	156:166	arg1	rate					142:145	the high basal metabolic rate	117:145	the high basal metabolic rate (BMR) of homeotherms	117:166	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	1	30	theme	homeotherms	156:166	arg1	by-product					192:201	a by-product	190:201	a by-product of natural selection for effective parental care	190:250	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	7	31	theme	between-line	1085:1096	arg1	differences					1098:1108	all the above between-line differences	1071:1108	all the above between-line differences	1071:1108	Although all the above between-line differences were statistically significant, their magnitude was too small to unambiguously ascribe them as stemming from a positive genetic correlation between the physiological traits underlying BMR and lactation performance.
25452500	7	31	theme	between-line	1085:1096	arg1	significant					1129:1139	significant	1129:1139	significant	1129:1139	Although all the above between-line differences were statistically significant, their magnitude was too small to unambiguously ascribe them as stemming from a positive genetic correlation between the physiological traits underlying BMR and lactation performance.
25452500	2	32	theme	milk	272:275	arg1	output					277:282	daily milk output	266:282	daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR	266:367	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	8	33	theme	such	1355:1358	arg1	support					1360:1366	such support	1355:1366	such support	1355:1366	Nevertheless, our study lends such support at least at the level of phenotypic variation.
25452500	6	34	theme	–	1048:1048	arg1	higher					1054:1059	higher	1054:1059	higher	1054:1059	However, the concentration of the primary milk carbohydrate – lactose – was higher.
25452500	6	34	theme	–	1048:1048	arg1	concentration					991:1003	the concentration	987:1003	the concentration of the primary milk carbohydrate – lactose –	987:1048	However, the concentration of the primary milk carbohydrate – lactose – was higher.
25452500	8	35	theme	phenotypic	1393:1402	arg1	variation					1404:1412	phenotypic variation	1393:1412	phenotypic variation	1393:1412	Nevertheless, our study lends such support at least at the level of phenotypic variation.
25452500	2	36	theme	daily	266:270	arg1	output					277:282	daily milk output	266:282	daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR	266:367	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	2	37	theme	cross-fostered	383:396	arg1	design					398:403	a cross-fostered design	381:403	a cross-fostered design	381:403	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	2	38	theme	between-line	449:460	arg1	abilities					471:479	the between-line suckling abilities	445:479	the between-line suckling abilities of nursed juveniles	445:499	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	7	39	theme	positive	1221:1228	arg1	correlation					1238:1248	a positive genetic correlation	1219:1248	a positive genetic correlation between the physiological traits underlying BMR and lactation performance	1219:1322	Although all the above between-line differences were statistically significant, their magnitude was too small to unambiguously ascribe them as stemming from a positive genetic correlation between the physiological traits underlying BMR and lactation performance.
25452500	3	40	theme	mother	577:582	arg1	mice					584:587	mother mice	577:587	mother mice	577:587	Additionally, to remedy the potential limitation imposed by the ability of mother mice to dissipate excess heat, we exposed them to an ambient temperature of 17°C during the most energetically demanding second week of lactation.
25452500	7	41	theme	genetic	1230:1236	arg1	correlation					1238:1248	a positive genetic correlation	1219:1248	a positive genetic correlation between the physiological traits underlying BMR and lactation performance	1219:1322	Although all the above between-line differences were statistically significant, their magnitude was too small to unambiguously ascribe them as stemming from a positive genetic correlation between the physiological traits underlying BMR and lactation performance.
25452500	7	42	theme	above	1079:1083	arg1	differences					1098:1108	all the above between-line differences	1071:1108	all the above between-line differences	1071:1108	Although all the above between-line differences were statistically significant, their magnitude was too small to unambiguously ascribe them as stemming from a positive genetic correlation between the physiological traits underlying BMR and lactation performance.
25452500	7	42	theme	above	1079:1083	arg1	significant					1129:1139	significant	1129:1139	significant	1129:1139	Although all the above between-line differences were statistically significant, their magnitude was too small to unambiguously ascribe them as stemming from a positive genetic correlation between the physiological traits underlying BMR and lactation performance.
25452500	3	43	theme	mice	584:587	arg1	ability					566:572	the ability	562:572	the ability of mother mice to dissipate excess heat	562:612	Additionally, to remedy the potential limitation imposed by the ability of mother mice to dissipate excess heat, we exposed them to an ambient temperature of 17°C during the most energetically demanding second week of lactation.
25452500	8	44	theme	variation	1404:1412	arg1	level					1384:1388	the level	1380:1388	the level of phenotypic variation	1380:1412	Nevertheless, our study lends such support at least at the level of phenotypic variation.
25452500	4	45	theme	more	799:802	arg1	milk					804:807	significantly more milk	785:807	significantly more milk	785:807	We found that the mice selected for high BMR produced significantly more milk in a 24 h period in both reproductive bouts.
25452500	6	46	theme	lactose	1040:1046	arg1	–					1048:1048	the primary milk carbohydrate – lactose –	1008:1048	the primary milk carbohydrate – lactose –	1008:1048	However, the concentration of the primary milk carbohydrate – lactose – was higher.
25452500	4	47	from	period	819:824	arg1	bouts					847:851	both reproductive bouts	829:851	both reproductive bouts	829:851	We found that the mice selected for high BMR produced significantly more milk in a 24 h period in both reproductive bouts.
25452500	4	48	theme	reproductive	834:845	arg1	bouts					847:851	both reproductive bouts	829:851	both reproductive bouts	829:851	We found that the mice selected for high BMR produced significantly more milk in a 24 h period in both reproductive bouts.
25452500	3	49	theme	ambient	637:643	arg1	temperature					645:655	an ambient temperature	634:655	an ambient temperature of 17°C	634:663	Additionally, to remedy the potential limitation imposed by the ability of mother mice to dissipate excess heat, we exposed them to an ambient temperature of 17°C during the most energetically demanding second week of lactation.
25452500	2	50	theme	lactation	307:315	arg1	bouts					317:321	two consecutive lactation bouts	291:321	two consecutive lactation bouts in mice divergently selected for high/low BMR	291:367	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	3	51	theme	excess	602:607	arg1	heat					609:612	excess heat	602:612	excess heat	602:612	Additionally, to remedy the potential limitation imposed by the ability of mother mice to dissipate excess heat, we exposed them to an ambient temperature of 17°C during the most energetically demanding second week of lactation.
25452500	2	52	theme	consecutive	295:305	arg1	bouts					317:321	two consecutive lactation bouts	291:321	two consecutive lactation bouts in mice divergently selected for high/low BMR	291:367	We estimated daily milk output during two consecutive lactation bouts in mice divergently selected for high/low BMR and applied a cross-fostered design to control for potential differences in the between-line suckling abilities of nursed juveniles.
25452500	5	53	theme	BMR	894:896	arg1	females					898:904	the high BMR females	885:904	the high BMR females	885:904	The milk samples obtained from the high BMR females had lower protein concentration and did not differ with respect to fat.
25452500	5	54	theme	milk	858:861	arg1	samples					863:869	The milk samples	854:869	The milk samples obtained from the high BMR females	854:904	The milk samples obtained from the high BMR females had lower protein concentration and did not differ with respect to fat.
25452500	1	55	theme	high	121:124	arg1	BMR					148:150	BMR	148:150	BMR	148:150	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	1	55	theme	high	121:124	arg1	rate					142:145	the high basal metabolic rate	117:145	the high basal metabolic rate (BMR) of homeotherms	117:166	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	1	55	theme	high	121:124	arg1	by-product					192:201	a by-product	190:201	a by-product of natural selection for effective parental care	190:250	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	0	56	theme	basal	61:65	arg1	rate					77:80	basal metabolic rate	61:80	basal metabolic rate	61:80	Milk output and composition in mice divergently selected for basal metabolic rate.
25452500	6	57	theme	–	1038:1038	arg1	–					1048:1048	the primary milk carbohydrate – lactose –	1008:1048	the primary milk carbohydrate – lactose –	1008:1048	However, the concentration of the primary milk carbohydrate – lactose – was higher.
25452500	4	58	theme	high	767:770	arg1	BMR					772:774	high BMR	767:774	high BMR	767:774	We found that the mice selected for high BMR produced significantly more milk in a 24 h period in both reproductive bouts.
25452500	1	59	theme	basal	126:130	arg1	BMR					148:150	BMR	148:150	BMR	148:150	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	1	59	theme	basal	126:130	arg1	rate					142:145	the high basal metabolic rate	117:145	the high basal metabolic rate (BMR) of homeotherms	117:166	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	1	59	theme	basal	126:130	arg1	by-product					192:201	a by-product	190:201	a by-product of natural selection for effective parental care	190:250	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	0	60	from	composition	16:26	arg1	mice					31:34	mice	31:34	mice	31:34	Milk output and composition in mice divergently selected for basal metabolic rate.
25452500	7	61	theme	lactation	1302:1310	arg1	performance					1312:1322	BMR and lactation performance	1294:1322	BMR and lactation performance	1294:1322	Although all the above between-line differences were statistically significant, their magnitude was too small to unambiguously ascribe them as stemming from a positive genetic correlation between the physiological traits underlying BMR and lactation performance.
25452500	1	62	theme	metabolic	132:140	arg1	BMR					148:150	BMR	148:150	BMR	148:150	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	1	62	theme	metabolic	132:140	arg1	rate					142:145	the high basal metabolic rate	117:145	the high basal metabolic rate (BMR) of homeotherms	117:166	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	1	62	theme	metabolic	132:140	arg1	by-product					192:201	a by-product	190:201	a by-product of natural selection for effective parental care	190:250	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
25452500	1	63	theme	natural	206:212	arg1	selection					214:222	natural selection	206:222	natural selection	206:222	From an evolutionary perspective, the high basal metabolic rate (BMR) of homeotherms is hypothesised to be a by-product of natural selection for effective parental care.
29224878	2	0	from	days	496:499	arg1	day					517:519	test day	512:519	test day	512:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	0	from	days	496:499	arg1	milk					504:507	milk	504:507	milk on test day	504:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	3	1	dep	Raleigh	793:799	arg1	NC					802:803	NC	802:803	NC	802:803	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	2	2	theme	test	512:515	arg1	day					517:519	test day	512:519	test day	512:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	3	3	theme	Management	773:782	arg1	Raleigh					793:799	Raleigh	793:799	Raleigh	793:799	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	3	3	theme	Management	773:782	arg1	Systems					784:790	Dairy Records Management Systems	759:790	Dairy Records Management Systems (Raleigh, NC)	759:804	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	12	4	theme	cow	2057:2059	arg1	MUN					2061:2063	individual cow MUN	2046:2063	individual cow MUN in Northeast dairy herds	2046:2088	Thus, we conclude that individual cow MUN in Northeast dairy herds fluctuated between 2004 and 2015.
29224878	2	5	from	milk	504:507	arg1	day					517:519	test day	512:519	test day	512:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	3	6	theme	DHIA	624:627	arg1	records					639:645	10,839,461 DHIA dairy cow records	613:645	10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV)	613:734	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	1	7	from	herds	266:270	arg1	States					299:304	the northeastern United States	275:304	the northeastern United States (the Northeast)	275:320	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	1	7	from	herds	266:270	arg1	Northeast					311:319	the Northeast	307:319	the Northeast	307:319	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	1	8	theme	potential	383:391	arg1	relationships					393:405	potential relationships	383:405	potential relationships for MUN and diet composition	383:434	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	13	9	theme	ingredient	2201:2210	arg1	data					2212:2215	ration feed ingredient data	2189:2215	ration feed ingredient data	2189:2215	It appeared that MUN followed variability in feed cost; however, ration feed ingredient data were not available to better define the reasons for the variations in MUN.
29224878	1	10	theme	Herd	230:233	arg1	herds					266:270	Dairy Herd Improvement Association (DHIA) herds	224:270	Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast)	224:320	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	3	11	theme	Dairy	759:763	arg1	Raleigh					793:799	Raleigh	793:799	Raleigh	793:799	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	3	11	theme	Dairy	759:763	arg1	Systems					784:790	Dairy Records Management Systems	759:790	Dairy Records Management Systems (Raleigh, NC)	759:804	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	9	12	theme	soluble	1679:1685	arg1	concentration					1695:1707	soluble protein concentration	1679:1707	soluble protein concentration	1679:1707	Concentration of TMR crude protein (CP) decreased from 17.1% in 2007 to 16.4% in 2015, but there was not an obvious trend in soluble protein concentration.
29224878	10	13	from	increase	1872:1879	arg1	concentration					1895:1907	TMR starch concentration	1884:1907	TMR starch concentration	1884:1907	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	0	14	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.	0:129	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	1	15	theme	survey	158:163	arg1	objective					140:148	The main objective	131:148	The main objective of this survey	131:163	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	2	16	theme	lactation	526:534	arg1	number					536:541	lactation number	526:541	lactation number of the cows	526:553	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	8	17	theme	Valley	1485:1490	arg1	database					1530:1537	the Cumberland Valley Analytical Service (Maugansville, MD) database	1470:1537	database	1530:1537	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	8	18	theme	composition	1349:1359	arg1	survey					1361:1366	the diet composition survey	1340:1366	the diet composition survey	1340:1366	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	3	19	theme	cow	635:637	arg1	records					639:645	10,839,461 DHIA dairy cow records	613:645	10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV)	613:734	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	0	20	theme	northeastern	103:114	arg1	States					123:128	the northeastern United States	99:128	the northeastern United States	99:128	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	3	21	dep	states	672:677	arg1	CT					680:681	CT	680:681	CT	680:681	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	13	22	theme	ration	2189:2194	arg1	data					2212:2215	ration feed ingredient data	2189:2215	ration feed ingredient data	2189:2215	It appeared that MUN followed variability in feed cost; however, ration feed ingredient data were not available to better define the reasons for the variations in MUN.
29224878	7	23	theme	high	1266:1269	arg1	MUN					1271:1273	high MUN	1266:1273	high MUN	1266:1273	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	4	24	theme	milk	841:844	arg1	yield					846:850	Average (across states and years) milk yield	807:850	Average (across states and years) milk yield	807:850	Average (across states and years) milk yield, milk fat, and milk protein were 31.6 ± 0.24 kg/d, 3.85 ± 0.021%, and 3.13 ± 0.013%, respectively.
29224878	8	25	theme	Maugansville	1512:1523	arg1	database					1530:1537	the Cumberland Valley Analytical Service (Maugansville, MD) database	1470:1537	database	1530:1537	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	3	26	dep	CT	680:681	arg1	MD					692:693	MD	692:693	MD	692:693	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	3	26	dep	CT	680:681	arg1	VA					720:721	VA	720:721	VA	720:721	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	3	26	dep	CT	680:681	arg1	ME					696:697	ME	696:697	ME	696:697	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	3	26	dep	CT	680:681	arg1	PA					712:713	PA	712:713	PA	712:713	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	3	26	dep	CT	680:681	arg1	VT					724:725	VT	724:725	VT	724:725	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	3	26	dep	CT	680:681	arg1	WV					732:733	WV	732:733	WV	732:733	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	3	26	dep	CT	680:681	arg1	NJ					704:705	NJ	704:705	NJ	704:705	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	3	26	dep	CT	680:681	arg1	RI					716:717	RI	716:717	RI	716:717	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	9	27	from	trend	1670:1674	arg1	concentration					1695:1707	soluble protein concentration	1679:1707	soluble protein concentration	1679:1707	Concentration of TMR crude protein (CP) decreased from 17.1% in 2007 to 16.4% in 2015, but there was not an obvious trend in soluble protein concentration.
29224878	1	28	theme	milk	195:198	arg1	MUN					215:217	MUN	215:217	MUN	215:217	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	1	28	theme	milk	195:198	arg1	nitrogen					205:212	milk urea nitrogen	195:212	milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast)	195:320	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	10	29	theme	TMR	1884:1886	arg1	concentration					1895:1907	TMR starch concentration	1884:1907	TMR starch concentration	1884:1907	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	2	30	from	Trends	437:442	arg1	days					496:499	days	496:499	days in milk on test day	496:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	30	from	Trends	437:442	arg1	concentrations					468:481	milk fat and protein concentrations	447:481	milk fat and protein concentrations	447:481	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	30	from	Trends	437:442	arg1	yield					489:493	milk yield	484:493	milk yield	484:493	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	30	from	Trends	437:442	arg1	number					536:541	lactation number	526:541	lactation number of the cows	526:553	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	1	31	theme	cow	347:349	arg1	composition					356:366	dairy cow diet composition	341:366	dairy cow diet composition	341:366	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	8	32	theme	total	1386:1390	arg1	ration					1398:1403	total mixed ration	1386:1403	total mixed ration (TMR) analyses	1386:1418	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	8	32	theme	total	1386:1390	arg1	TMR					1406:1408	TMR	1406:1408	TMR	1406:1408	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	10	33	dep	in	1770:1771	arg1	vitro					1773:1777	vitro	1773:1777	vitro	1773:1777	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	10	34	theme	24-h	1765:1768	arg1	degradability					1783:1795	24-h in vitro NDF degradability	1765:1795	24-h in vitro NDF degradability	1765:1795	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	9	35	theme	TMR	1571:1573	arg1	CP					1590:1591	CP	1590:1591	CP	1590:1591	Concentration of TMR crude protein (CP) decreased from 17.1% in 2007 to 16.4% in 2015, but there was not an obvious trend in soluble protein concentration.
29224878	9	35	theme	TMR	1571:1573	arg1	protein					1581:1587	TMR crude protein	1571:1587	TMR crude protein (CP)	1571:1592	Concentration of TMR crude protein (CP) decreased from 17.1% in 2007 to 16.4% in 2015, but there was not an obvious trend in soluble protein concentration.
29224878	8	36	theme	ration	1398:1403	arg1	analyses					1411:1418	total mixed ration (TMR) analyses	1386:1418	total mixed ration (TMR) analyses	1386:1418	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	9	37	theme	crude	1575:1579	arg1	CP					1590:1591	CP	1590:1591	CP	1590:1591	Concentration of TMR crude protein (CP) decreased from 17.1% in 2007 to 16.4% in 2015, but there was not an obvious trend in soluble protein concentration.
29224878	9	37	theme	crude	1575:1579	arg1	protein					1581:1587	TMR crude protein	1571:1587	TMR crude protein (CP)	1571:1592	Concentration of TMR crude protein (CP) decreased from 17.1% in 2007 to 16.4% in 2015, but there was not an obvious trend in soluble protein concentration.
29224878	5	38	theme	obvious	954:960	arg1	trends					962:967	No obvious trends	951:967	No obvious trends	951:967	No obvious trends were observed for milk fat or protein content, but milk yield steadily increased during the survey period.
29224878	13	39	theme	feed	2169:2172	arg1	cost					2174:2177	feed cost	2169:2177	feed cost	2169:2177	It appeared that MUN followed variability in feed cost; however, ration feed ingredient data were not available to better define the reasons for the variations in MUN.
29224878	1	40	theme	northeastern	279:290	arg1	States					299:304	the northeastern United States	275:304	the northeastern United States (the Northeast)	275:320	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	1	40	theme	northeastern	279:290	arg1	Northeast					311:319	the Northeast	307:319	the Northeast	307:319	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	1	41	theme	main	135:138	arg1	objective					140:148	The main objective	131:148	The main objective of this survey	131:163	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	9	42	dep	16.4	1626:1629	arg1	to					1623:1624	to	1623:1624	to	1623:1624	Concentration of TMR crude protein (CP) decreased from 17.1% in 2007 to 16.4% in 2015, but there was not an obvious trend in soluble protein concentration.
29224878	10	43	theme	TMR	1727:1729	arg1	NDF					1756:1758	NDF	1756:1758	NDF	1756:1758	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	10	43	theme	TMR	1727:1729	arg1	fiber					1749:1753	TMR neutral detergent fiber	1727:1753	TMR neutral detergent fiber (NDF)	1727:1759	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	0	44	theme	total	65:69	arg1	composition					84:94	dairy total mixed ration composition	59:94	dairy total mixed ration composition	59:94	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	3	45	dep	MD	692:693	arg1	DE					684:685	DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV	684:733	DE	684:685	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	2	46	theme	protein	460:466	arg1	concentrations					468:481	milk fat and protein concentrations	447:481	milk fat and protein concentrations	447:481	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	11	47	theme	chemical	2001:2008	arg1	composition					2010:2020	diet chemical composition	1996:2020	diet chemical composition	1996:2020	Examination of these unrelated data sets revealed lack of correlation between MUN and diet chemical composition.
29224878	0	48	theme	ration	77:82	arg1	composition					84:94	dairy total mixed ration composition	59:94	dairy total mixed ration composition	59:94	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	8	49	theme	unrelated	1421:1429	arg1	analyses					1411:1418	total mixed ration (TMR) analyses	1386:1418	total mixed ration (TMR) analyses	1386:1418	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	7	50	theme	high	1319:1322	arg1	price					1329:1333	high milk price	1319:1333	high milk price	1319:1333	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	7	51	theme	dairy	1198:1202	arg1	cost					1209:1212	dairy feed cost	1198:1212	dairy feed cost	1198:1212	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	2	52	theme	fat	452:454	arg1	concentrations					468:481	milk fat and protein concentrations	447:481	milk fat and protein concentrations	447:481	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	1	53	from	trends	331:336	arg1	composition					356:366	dairy cow diet composition	341:366	dairy cow diet composition	341:366	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	11	54	theme	data	1941:1944	arg1	sets					1946:1949	these unrelated data sets	1925:1949	these unrelated data sets	1925:1949	Examination of these unrelated data sets revealed lack of correlation between MUN and diet chemical composition.
29224878	5	55	theme	protein	999:1005	arg1	content					1007:1013	protein content	999:1013	protein content	999:1013	No obvious trends were observed for milk fat or protein content, but milk yield steadily increased during the survey period.
29224878	6	56	theme	N	1086:1086	arg1	concentration					1088:1100	Milk urea N concentration	1076:1100	Milk urea N concentration	1076:1100	Milk urea N concentration averaged 13.3 ± 0.13 mg/dL, with no obvious or consistent trends.
29224878	1	57	theme	Improvement	235:245	arg1	herds					266:270	Dairy Herd Improvement Association (DHIA) herds	224:270	Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast)	224:320	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	8	58	theme	composition	1443:1453	arg1	data					1455:1458	the milk composition data	1434:1458	the milk composition data set	1434:1462	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	7	59	theme	high	1300:1303	arg1	cost					1310:1313	high feed cost	1300:1313	high feed cost	1300:1313	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	4	60	theme	±	908:908	arg1	%					915:915	31.6 ± 0.24 kg/d, 3.85 ± 0.021%	885:915	%	915:915	Average (across states and years) milk yield, milk fat, and milk protein were 31.6 ± 0.24 kg/d, 3.85 ± 0.021%, and 3.13 ± 0.013%, respectively.
29224878	6	61	theme	Milk	1076:1079	arg1	concentration					1088:1100	Milk urea N concentration	1076:1100	Milk urea N concentration	1076:1100	Milk urea N concentration averaged 13.3 ± 0.13 mg/dL, with no obvious or consistent trends.
29224878	11	62	theme	sets	1946:1949	arg1	Examination					1910:1920	Examination	1910:1920	Examination of these unrelated data sets	1910:1949	Examination of these unrelated data sets revealed lack of correlation between MUN and diet chemical composition.
29224878	2	63	from	day	517:519	arg1	days					496:499	days	496:499	days in milk on test day	496:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	63	from	day	517:519	arg1	concentrations					468:481	milk fat and protein concentrations	447:481	milk fat and protein concentrations	447:481	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	63	from	day	517:519	arg1	yield					489:493	milk yield	484:493	milk yield	484:493	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	63	from	day	517:519	arg1	number					536:541	lactation number	526:541	lactation number of the cows	526:553	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	7	64	from	cost	1209:1212	arg1	Examination					1168:1178	Examination	1168:1178	Examination of variability in dairy feed cost	1168:1212	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	7	64	from	cost	1209:1212	arg1	price					1227:1231	all milk price	1218:1231	all milk price for the Northeast	1218:1249	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	0	65	theme	urea	41:44	arg1	nitrogen					46:53	milk urea nitrogen	36:53	milk urea nitrogen	36:53	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	12	66	theme	Northeast	2068:2076	arg1	herds					2084:2088	Northeast dairy herds	2068:2088	Northeast dairy herds	2068:2088	Thus, we conclude that individual cow MUN in Northeast dairy herds fluctuated between 2004 and 2015.
29224878	2	67	from	number	536:541	arg1	day					517:519	test day	512:519	test day	512:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	67	from	number	536:541	arg1	milk					504:507	milk	504:507	milk on test day	504:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	4	68	theme	±	927:927	arg1	%					934:934	3.13 ± 0.013%	922:934	3.13 ± 0.013%	922:934	Average (across states and years) milk yield, milk fat, and milk protein were 31.6 ± 0.24 kg/d, 3.85 ± 0.021%, and 3.13 ± 0.013%, respectively.
29224878	10	69	theme	neutral	1731:1737	arg1	NDF					1756:1758	NDF	1756:1758	NDF	1756:1758	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	10	69	theme	neutral	1731:1737	arg1	fiber					1749:1753	TMR neutral detergent fiber	1727:1753	TMR neutral detergent fiber (NDF)	1727:1759	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	1	70	theme	urea	200:203	arg1	MUN					215:217	MUN	215:217	MUN	215:217	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	1	70	theme	urea	200:203	arg1	nitrogen					205:212	milk urea nitrogen	195:212	milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast)	195:320	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	7	71	from	price	1227:1231	arg1	cost					1209:1212	dairy feed cost	1198:1212	dairy feed cost	1198:1212	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	2	72	from	concentrations	468:481	arg1	day					517:519	test day	512:519	test day	512:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	72	from	concentrations	468:481	arg1	milk					504:507	milk	504:507	milk on test day	504:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	12	73	theme	individual	2046:2055	arg1	MUN					2061:2063	individual cow MUN	2046:2063	individual cow MUN in Northeast dairy herds	2046:2088	Thus, we conclude that individual cow MUN in Northeast dairy herds fluctuated between 2004 and 2015.
29224878	5	74	theme	milk	1020:1023	arg1	yield					1025:1029	milk yield	1020:1029	milk yield	1020:1029	No obvious trends were observed for milk fat or protein content, but milk yield steadily increased during the survey period.
29224878	6	75	theme	±	1116:1116	arg1	mg/dL					1123:1127	13.3 ± 0.13 mg/dL	1111:1127	13.3 ± 0.13 mg/dL	1111:1127	Milk urea N concentration averaged 13.3 ± 0.13 mg/dL, with no obvious or consistent trends.
29224878	10	76	theme	survey	1826:1831	arg1	period					1833:1838	the survey period	1822:1838	the survey period	1822:1838	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	3	77	theme	Records	765:771	arg1	Raleigh					793:799	Raleigh	793:799	Raleigh	793:799	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	3	77	theme	Records	765:771	arg1	Systems					784:790	Dairy Records Management Systems	759:790	Dairy Records Management Systems (Raleigh, NC)	759:804	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	4	78	theme	Average	807:813	arg1	yield					846:850	Average (across states and years) milk yield	807:850	Average (across states and years) milk yield	807:850	Average (across states and years) milk yield, milk fat, and milk protein were 31.6 ± 0.24 kg/d, 3.85 ± 0.021%, and 3.13 ± 0.013%, respectively.
29224878	8	79	from	database	1530:1537	arg1	records					1375:1381	9,707 records	1369:1381	9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database	1369:1537	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	9	80	theme	obvious	1662:1668	arg1	trend					1670:1674	an obvious trend	1659:1674	an obvious trend in soluble protein concentration	1659:1707	Concentration of TMR crude protein (CP) decreased from 17.1% in 2007 to 16.4% in 2015, but there was not an obvious trend in soluble protein concentration.
29224878	1	81	theme	Dairy	224:228	arg1	herds					266:270	Dairy Herd Improvement Association (DHIA) herds	224:270	Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast)	224:320	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	7	82	from	Examination	1168:1178	arg1	cost					1209:1212	dairy feed cost	1198:1212	dairy feed cost	1198:1212	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	10	83	theme	degradability	1783:1795	arg1	Concentration					1710:1722	Concentration	1710:1722	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability	1710:1795	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	4	84	theme	milk	853:856	arg1	fat					858:860	milk fat	853:860	milk fat	853:860	Average (across states and years) milk yield, milk fat, and milk protein were 31.6 ± 0.24 kg/d, 3.85 ± 0.021%, and 3.13 ± 0.013%, respectively.
29224878	3	85	theme	dairy	629:633	arg1	records					639:645	10,839,461 DHIA dairy cow records	613:645	10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV)	613:734	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	8	86	theme	Cumberland	1474:1483	arg1	database					1530:1537	the Cumberland Valley Analytical Service (Maugansville, MD) database	1470:1537	database	1530:1537	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	7	87	from	variability	1183:1193	arg1	cost					1209:1212	dairy feed cost	1198:1212	dairy feed cost	1198:1212	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	10	88	theme	starch	1888:1893	arg1	concentration					1895:1907	TMR starch concentration	1884:1907	TMR starch concentration	1884:1907	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	11	89	theme	correlation	1968:1978	arg1	lack					1960:1963	lack	1960:1963	lack of correlation between MUN and diet chemical composition	1960:2020	Examination of these unrelated data sets revealed lack of correlation between MUN and diet chemical composition.
29224878	9	90	theme	protein	1687:1693	arg1	concentration					1695:1707	soluble protein concentration	1679:1707	soluble protein concentration	1679:1707	Concentration of TMR crude protein (CP) decreased from 17.1% in 2007 to 16.4% in 2015, but there was not an obvious trend in soluble protein concentration.
29224878	8	91	theme	Analytical	1492:1501	arg1	database					1530:1537	the Cumberland Valley Analytical Service (Maugansville, MD) database	1470:1537	database	1530:1537	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	5	92	theme	survey	1061:1066	arg1	period					1068:1073	the survey period	1057:1073	the survey period	1057:1073	No obvious trends were observed for milk fat or protein content, but milk yield steadily increased during the survey period.
29224878	0	93	theme	United	116:121	arg1	States					123:128	the northeastern United States	99:128	the northeastern United States	99:128	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	1	94	theme	diet	419:422	arg1	composition					424:434	diet composition	419:434	diet composition	419:434	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	6	95	theme	consistent	1149:1158	arg1	trends					1160:1165	no obvious or consistent trends	1135:1165	no obvious or consistent trends	1135:1165	Milk urea N concentration averaged 13.3 ± 0.13 mg/dL, with no obvious or consistent trends.
29224878	2	96	theme	milk	484:487	arg1	yield					489:493	milk yield	484:493	milk yield	484:493	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	6	97	theme	obvious	1138:1144	arg1	trends					1160:1165	no obvious or consistent trends	1135:1165	no obvious or consistent trends	1135:1165	Milk urea N concentration averaged 13.3 ± 0.13 mg/dL, with no obvious or consistent trends.
29224878	4	98	theme	±	890:890	arg1	%					915:915	31.6 ± 0.24 kg/d, 3.85 ± 0.021%	885:915	%	915:915	Average (across states and years) milk yield, milk fat, and milk protein were 31.6 ± 0.24 kg/d, 3.85 ± 0.021%, and 3.13 ± 0.013%, respectively.
29224878	1	99	theme	dairy	341:345	arg1	composition					356:366	dairy cow diet composition	341:366	dairy cow diet composition	341:366	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	4	100	theme	kg/d	897:900	arg1	%					915:915	31.6 ± 0.24 kg/d, 3.85 ± 0.021%	885:915	%	915:915	Average (across states and years) milk yield, milk fat, and milk protein were 31.6 ± 0.24 kg/d, 3.85 ± 0.021%, and 3.13 ± 0.013%, respectively.
29224878	1	101	theme	diet	351:354	arg1	composition					356:366	dairy cow diet composition	341:366	dairy cow diet composition	341:366	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	1	102	from	variability	180:190	arg1	MUN					215:217	MUN	215:217	MUN	215:217	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	1	102	from	variability	180:190	arg1	nitrogen					205:212	milk urea nitrogen	195:212	milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast)	195:320	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	7	103	theme	milk	1222:1225	arg1	price					1227:1231	all milk price	1218:1231	all milk price for the Northeast	1218:1249	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	10	104	theme	steady	1865:1870	arg1	increase					1872:1879	a steady increase	1863:1879	a steady increase in TMR starch concentration	1863:1907	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	4	105	theme	milk	867:870	arg1	protein					872:878	milk protein	867:878	milk protein	867:878	Average (across states and years) milk yield, milk fat, and milk protein were 31.6 ± 0.24 kg/d, 3.85 ± 0.021%, and 3.13 ± 0.013%, respectively.
29224878	8	106	theme	diet	1344:1347	arg1	composition					1349:1359	the diet composition	1340:1359	the diet composition survey	1340:1366	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	0	107	theme	dairy	59:63	arg1	composition					84:94	dairy total mixed ration composition	59:94	dairy total mixed ration composition	59:94	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	8	108	theme	mixed	1392:1396	arg1	ration					1398:1403	total mixed ration	1386:1403	total mixed ration (TMR) analyses	1386:1418	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	8	108	theme	mixed	1392:1396	arg1	TMR					1406:1408	TMR	1406:1408	TMR	1406:1408	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	0	109	from	Variability	21:31	arg1	States					123:128	the northeastern United States	99:128	the northeastern United States	99:128	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	0	109	from	Variability	21:31	arg1	composition					84:94	dairy total mixed ration composition	59:94	dairy total mixed ration composition	59:94	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	0	109	from	Variability	21:31	arg1	nitrogen					46:53	milk urea nitrogen	36:53	milk urea nitrogen	36:53	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	9	110	theme	protein	1581:1587	arg1	Concentration					1554:1566	Concentration	1554:1566	Concentration of TMR crude protein (CP)	1554:1592	Concentration of TMR crude protein (CP) decreased from 17.1% in 2007 to 16.4% in 2015, but there was not an obvious trend in soluble protein concentration.
29224878	0	111	theme	mixed	71:75	arg1	composition					84:94	dairy total mixed ration composition	59:94	dairy total mixed ration composition	59:94	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	1	112	theme	United	292:297	arg1	States					299:304	the northeastern United States	275:304	the northeastern United States (the Northeast)	275:320	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	1	112	theme	United	292:297	arg1	Northeast					311:319	the Northeast	307:319	the Northeast	307:319	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	7	113	theme	feed	1204:1207	arg1	cost					1209:1212	dairy feed cost	1198:1212	dairy feed cost	1198:1212	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	8	114	theme	Service	1503:1509	arg1	database					1530:1537	the Cumberland Valley Analytical Service (Maugansville, MD) database	1470:1537	database	1530:1537	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	11	115	theme	diet	1996:1999	arg1	composition					2010:2020	diet chemical composition	1996:2020	diet chemical composition	1996:2020	Examination of these unrelated data sets revealed lack of correlation between MUN and diet chemical composition.
29224878	3	116	from	2015	660:663	arg1	records					639:645	10,839,461 DHIA dairy cow records	613:645	10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV)	613:734	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	9	117	from	%	1630:1630	arg1	2015					1635:1638	2015	1635:1638	2015	1635:1638	Concentration of TMR crude protein (CP) decreased from 17.1% in 2007 to 16.4% in 2015, but there was not an obvious trend in soluble protein concentration.
29224878	4	118	theme	3.13	922:925	arg1	%					934:934	3.13 ± 0.013%	922:934	3.13 ± 0.013%	922:934	Average (across states and years) milk yield, milk fat, and milk protein were 31.6 ± 0.24 kg/d, 3.85 ± 0.021%, and 3.13 ± 0.013%, respectively.
29224878	2	119	theme	cows	550:553	arg1	days					496:499	days	496:499	days in milk on test day	496:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	119	theme	cows	550:553	arg1	concentrations					468:481	milk fat and protein concentrations	447:481	milk fat and protein concentrations	447:481	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	119	theme	cows	550:553	arg1	yield					489:493	milk yield	484:493	milk yield	484:493	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	119	theme	cows	550:553	arg1	number					536:541	lactation number	526:541	lactation number of the cows	526:553	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	5	120	theme	milk	987:990	arg1	fat					992:994	milk fat	987:994	milk fat	987:994	No obvious trends were observed for milk fat or protein content, but milk yield steadily increased during the survey period.
29224878	3	121	dep	2015	660:663	arg1	to					657:658	to	657:658	to	657:658	The data set for the survey included 10,839,461 DHIA dairy cow records from 2004 to 2015 for 13 states (CT, DE, MA, MD, ME, NH, NJ, NY, PA, RI, VA, VT, and WV) and was retrieved from Dairy Records Management Systems (Raleigh, NC).
29224878	10	122	theme	detergent	1739:1747	arg1	NDF					1756:1758	NDF	1756:1758	NDF	1756:1758	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	10	122	theme	detergent	1739:1747	arg1	fiber					1749:1753	TMR neutral detergent fiber	1727:1753	TMR neutral detergent fiber (NDF)	1727:1759	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	7	123	theme	milk	1324:1327	arg1	price					1329:1333	high milk price	1319:1333	high milk price	1319:1333	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	2	124	theme	milk	447:450	arg1	fat					452:454	milk fat	447:454	milk fat	447:454	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	10	125	theme	NDF	1779:1781	arg1	degradability					1783:1795	24-h in vitro NDF degradability	1765:1795	24-h in vitro NDF degradability	1765:1795	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	8	126	dep	database	1530:1537	arg1	MD					1526:1527	the Cumberland Valley Analytical Service (Maugansville, MD) database	1470:1537	MD	1526:1527	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	10	127	theme	in	1770:1771	arg1	degradability					1783:1795	24-h in vitro NDF degradability	1765:1795	24-h in vitro NDF degradability	1765:1795	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
29224878	6	128	theme	urea	1081:1084	arg1	concentration					1088:1100	Milk urea N concentration	1076:1100	Milk urea N concentration	1076:1100	Milk urea N concentration averaged 13.3 ± 0.13 mg/dL, with no obvious or consistent trends.
29224878	1	129	theme	Association	247:257	arg1	herds					266:270	Dairy Herd Improvement Association (DHIA) herds	224:270	Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast)	224:320	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	8	130	theme	milk	1438:1441	arg1	data					1455:1458	the milk composition data	1434:1458	the milk composition data set	1434:1462	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	7	131	theme	feed	1305:1308	arg1	cost					1310:1313	high feed cost	1300:1313	high feed cost	1300:1313	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	13	132	theme	feed	2196:2199	arg1	data					2212:2215	ration feed ingredient data	2189:2215	ration feed ingredient data	2189:2215	It appeared that MUN followed variability in feed cost; however, ration feed ingredient data were not available to better define the reasons for the variations in MUN.
29224878	0	133	theme	milk	36:39	arg1	nitrogen					46:53	milk urea nitrogen	36:53	milk urea nitrogen	36:53	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	1	134	theme	DHIA	260:263	arg1	herds					266:270	Dairy Herd Improvement Association (DHIA) herds	224:270	Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast)	224:320	The main objective of this survey was to examine variability in milk urea nitrogen (MUN) for Dairy Herd Improvement Association (DHIA) herds in the northeastern United States (the Northeast), examine trends in dairy cow diet composition, and determine potential relationships for MUN and diet composition.
29224878	7	135	theme	variability	1183:1193	arg1	Examination					1168:1178	Examination	1168:1178	Examination of variability in dairy feed cost	1168:1212	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	7	135	theme	variability	1183:1193	arg1	price					1227:1231	all milk price	1218:1231	all milk price for the Northeast	1218:1249	Examination of variability in dairy feed cost and all milk price for the Northeast indicated that high MUN generally coincided with high feed cost and high milk price.
29224878	11	136	theme	unrelated	1931:1939	arg1	sets					1946:1949	these unrelated data sets	1925:1949	these unrelated data sets	1925:1949	Examination of these unrelated data sets revealed lack of correlation between MUN and diet chemical composition.
29224878	12	137	from	MUN	2061:2063	arg1	herds					2084:2088	Northeast dairy herds	2068:2088	Northeast dairy herds	2068:2088	Thus, we conclude that individual cow MUN in Northeast dairy herds fluctuated between 2004 and 2015.
29224878	13	138	from	variations	2273:2282	arg1	MUN					2287:2289	MUN	2287:2289	MUN	2287:2289	It appeared that MUN followed variability in feed cost; however, ration feed ingredient data were not available to better define the reasons for the variations in MUN.
29224878	8	139	theme	analyses	1411:1418	arg1	records					1375:1381	9,707 records	1369:1381	9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database	1369:1537	For the diet composition survey, 9,707 records of total mixed ration (TMR) analyses, unrelated to the milk composition data set, from the Cumberland Valley Analytical Service (Maugansville, MD) database were examined.
29224878	0	140	dep	communication	6:18	arg1	Variability					21:31	Variability	21:31	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.	0:129	Short communication: Variability in milk urea nitrogen and dairy total mixed ration composition in the northeastern United States.
29224878	12	141	theme	dairy	2078:2082	arg1	herds					2084:2088	Northeast dairy herds	2068:2088	Northeast dairy herds	2068:2088	Thus, we conclude that individual cow MUN in Northeast dairy herds fluctuated between 2004 and 2015.
29224878	2	142	from	yield	489:493	arg1	day					517:519	test day	512:519	test day	512:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	2	142	from	yield	489:493	arg1	milk					504:507	milk	504:507	milk on test day	504:519	Trends in milk fat and protein concentrations, milk yield, days in milk on test day, and lactation number of the cows were also evaluated.
29224878	10	143	theme	fiber	1749:1753	arg1	Concentration					1710:1722	Concentration	1710:1722	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability	1710:1795	Concentration of TMR neutral detergent fiber (NDF) and 24-h in vitro NDF degradability declined steadily during the survey period and was accompanied by a steady increase in TMR starch concentration.
24418313	12	0	theme	structural	1754:1763	arg1	function					1765:1772	a structural function	1752:1772	a structural function	1752:1772	Approximately 35% of the total proteins identified were classified as having a structural function.
24418313	13	1	theme	amino	1799:1803	arg1	catalase					1852:1859	catalase	1852:1859	catalase	1852:1859	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	13	1	theme	amino	1799:1803	arg1	protein					1790:1796	Chitin-binding protein	1775:1796	Chitin-binding protein	1775:1796	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	13	1	theme	amino	1799:1803	arg1	oxidase					1827:1833	amino acid oxidase	1816:1833	amino acid oxidase	1816:1833	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	13	1	theme	amino	1799:1803	arg1	peroxidase					1865:1874	peroxidase	1865:1874	peroxidase	1865:1874	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	13	1	theme	amino	1799:1803	arg1	peptidase					1805:1813	amino peptidase	1799:1813	amino peptidase	1799:1813	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	13	1	theme	amino	1799:1803	arg1	oxidoreductase					1836:1849	oxidoreductase	1836:1849	oxidoreductase	1836:1849	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	8	2	theme	neutral	1282:1288	arg1	lipids					1290:1295	neutral lipids	1282:1295	neutral lipids (64.12%)	1282:1304	In addition carbohydrates, we also identified neutral lipids (64.12%) and phospholipids (35.88%).
24418313	8	2	theme	neutral	1282:1288	arg1	%					1303:1303	64.12%	1298:1303	64.12%	1298:1303	In addition carbohydrates, we also identified neutral lipids (64.12%) and phospholipids (35.88%).
24418313	2	3	attach	derived	318:324	arg2	knowledge					281:289	the knowledge	277:289	the knowledge about the EC of insects	277:313	Most of the knowledge about the EC of insects is derived from studies on ultrastructure and secretion processes during embryonic development, and little is known about the molecular composition of this structure.
24418313	2	3	attach	derived	318:324	arg2	Most					269:272	Most	269:272	Most	269:272	Most of the knowledge about the EC of insects is derived from studies on ultrastructure and secretion processes during embryonic development, and little is known about the molecular composition of this structure.
24418313	2	3	attach	derived	318:324	arg1	studies					331:337	studies	331:337	studies on ultrastructure and secretion processes during embryonic development	331:408	Most of the knowledge about the EC of insects is derived from studies on ultrastructure and secretion processes during embryonic development, and little is known about the molecular composition of this structure.
24418313	9	4	dep	http	1475:1478	arg1	//www.vectorbase.org					1480:1499	//www.vectorbase.org	1480:1499	http://www.vectorbase.org	1475:1499	Proteomic analysis detected 68 proteins (55 were identified and 13 are hypothetical proteins) using the sequences in the R. prolixus genome (http://www.vectorbase.org).
24418313	7	5	theme	R. prolixus	1223:1233	arg1	EC					1217:1218	the EC	1213:1218	the EC of R. prolixus	1213:1233	In addition to chitin, we also identified amino (15%), neutral (12%) and acidic (3%) carbohydrates in the EC of R. prolixus.
24418313	14	6	theme	EC	2009:2010	arg1	function					1993:2000	the function	1989:2000	the function of the EC in assisting the insect during eclosion	1989:2050	Proteins known to be cuticle constituents may be related to the function of the EC in assisting the insect during eclosion.
24418313	1	7	theme	narrow	244:249	arg1	border					261:266	a narrow chorionic border	242:266	a narrow chorionic border	242:266	The embryonic cuticle (EC) of Rhodnius prolixus envelopes the entire body of the embryo during hatching and provides physical protection, allowing the embryo to pass through a narrow chorionic border.
24418313	2	8	theme	insects	307:313	arg1	EC					301:302	the EC	297:302	the EC of insects	297:313	Most of the knowledge about the EC of insects is derived from studies on ultrastructure and secretion processes during embryonic development, and little is known about the molecular composition of this structure.
24418313	9	9	theme	hypothetical	1405:1416	arg1	proteins					1418:1425	hypothetical proteins	1405:1425	hypothetical proteins	1405:1425	Proteomic analysis detected 68 proteins (55 were identified and 13 are hypothetical proteins) using the sequences in the R. prolixus genome (http://www.vectorbase.org).
24418313	2	10	theme	embryonic	388:396	arg1	development					398:408	embryonic development	388:408	embryonic development	388:408	Most of the knowledge about the EC of insects is derived from studies on ultrastructure and secretion processes during embryonic development, and little is known about the molecular composition of this structure.
24418313	1	11	theme	embryonic	72:80	arg1	EC					91:92	EC	91:92	EC	91:92	The embryonic cuticle (EC) of Rhodnius prolixus envelopes the entire body of the embryo during hatching and provides physical protection, allowing the embryo to pass through a narrow chorionic border.
24418313	1	11	theme	embryonic	72:80	arg1	cuticle					82:88	The embryonic cuticle	68:88	The embryonic cuticle (EC) of Rhodnius prolixus	68:114	The embryonic cuticle (EC) of Rhodnius prolixus envelopes the entire body of the embryo during hatching and provides physical protection, allowing the embryo to pass through a narrow chorionic border.
24418313	7	12	theme	amino	1153:1157	arg1	carbohydrates					1196:1208	amino (15%), neutral (12%) and acidic (3%) carbohydrates	1153:1208	amino (15%), neutral (12%) and acidic (3%) carbohydrates	1153:1208	In addition to chitin, we also identified amino (15%), neutral (12%) and acidic (3%) carbohydrates in the EC of R. prolixus.
24418313	1	13	theme	embryo	149:154	arg1	body					137:140	the entire body	126:140	the entire body of the embryo	126:154	The embryonic cuticle (EC) of Rhodnius prolixus envelopes the entire body of the embryo during hatching and provides physical protection, allowing the embryo to pass through a narrow chorionic border.
24418313	9	14	from	sequences	1438:1446	arg1	genome					1467:1472	the R. prolixus genome	1451:1472	the R. prolixus genome (http://www.vectorbase.org)	1451:1500	Proteomic analysis detected 68 proteins (55 were identified and 13 are hypothetical proteins) using the sequences in the R. prolixus genome (http://www.vectorbase.org).
24418313	3	15	theme	eclosion	683:690	arg1	process					692:698	the eclosion process	679:698	the eclosion process	679:698	We performed a comprehensive molecular characterization of the major components extracted from the EC of R. prolixus, and we discuss the role of the different molecules that were identified during the eclosion process.
24418313	7	16	dep	acidic	1184:1189	arg1	%					1193:1193	3%	1192:1193	3%	1192:1193	In addition to chitin, we also identified amino (15%), neutral (12%) and acidic (3%) carbohydrates in the EC of R. prolixus.
24418313	3	17	theme	molecular	511:519	arg1	characterization					521:536	a comprehensive molecular characterization	495:536	a comprehensive molecular characterization of the major components extracted from the EC of R. prolixus	495:597	We performed a comprehensive molecular characterization of the major components extracted from the EC of R. prolixus, and we discuss the role of the different molecules that were identified during the eclosion process.
24418313	9	18	dep	13	1398:1399	arg1	proteins					1418:1425	hypothetical proteins	1405:1425	hypothetical proteins	1405:1425	Proteomic analysis detected 68 proteins (55 were identified and 13 are hypothetical proteins) using the sequences in the R. prolixus genome (http://www.vectorbase.org).
24418313	3	19	theme	components	551:560	arg1	characterization					521:536	a comprehensive molecular characterization	495:536	a comprehensive molecular characterization of the major components extracted from the EC of R. prolixus	495:597	We performed a comprehensive molecular characterization of the major components extracted from the EC of R. prolixus, and we discuss the role of the different molecules that were identified during the eclosion process.
24418313	15	20	theme	global	2111:2116	arg1	composition					2128:2138	the global molecular composition	2107:2138	the global molecular composition of an EC in insects	2107:2158	To our knowledge, this is the first study to describe the global molecular composition of an EC in insects.
24418313	2	21	theme	ultrastructure	342:355	arg1	processes					371:379	ultrastructure and secretion processes	342:379	ultrastructure and secretion processes during embryonic development	342:408	Most of the knowledge about the EC of insects is derived from studies on ultrastructure and secretion processes during embryonic development, and little is known about the molecular composition of this structure.
24418313	0	22	theme	Molecular	0:8	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of Rhodnius prolixus	0:46	Molecular characterization of Rhodnius prolixus' embryonic cuticle.
24418313	1	23	theme	chorionic	251:259	arg1	border					261:266	a narrow chorionic border	242:266	a narrow chorionic border	242:266	The embryonic cuticle (EC) of Rhodnius prolixus envelopes the entire body of the embryo during hatching and provides physical protection, allowing the embryo to pass through a narrow chorionic border.
24418313	2	24	from	studies	331:337	arg1	processes					371:379	ultrastructure and secretion processes	342:379	ultrastructure and secretion processes during embryonic development	342:408	Most of the knowledge about the EC of insects is derived from studies on ultrastructure and secretion processes during embryonic development, and little is known about the molecular composition of this structure.
24418313	13	25	theme	amino	1816:1820	arg1	oxidase					1827:1833	amino acid oxidase	1816:1833	amino acid oxidase	1816:1833	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	13	25	theme	amino	1816:1820	arg1	peptidase					1805:1813	amino peptidase	1799:1813	amino peptidase	1799:1813	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	15	26	theme	molecular	2118:2126	arg1	composition					2128:2138	the global molecular composition	2107:2138	the global molecular composition of an EC in insects	2107:2158	To our knowledge, this is the first study to describe the global molecular composition of an EC in insects.
24418313	0	27	theme	Rhodnius	30:37	arg1	prolixus					39:46	Rhodnius prolixus	30:46	Rhodnius prolixus	30:46	Molecular characterization of Rhodnius prolixus' embryonic cuticle.
24418313	8	28	dep	carbohydrates	1248:1260	arg1	addition					1239:1246	addition	1239:1246	addition	1239:1246	In addition carbohydrates, we also identified neutral lipids (64.12%) and phospholipids (35.88%).
24418313	7	29	theme	acidic	1184:1189	arg1	amino					1153:1157	amino	1153:1157	amino (15%)	1153:1163	In addition to chitin, we also identified amino (15%), neutral (12%) and acidic (3%) carbohydrates in the EC of R. prolixus.
24418313	7	29	theme	acidic	1184:1189	arg1	%					1162:1162	15%	1160:1162	15%	1160:1162	In addition to chitin, we also identified amino (15%), neutral (12%) and acidic (3%) carbohydrates in the EC of R. prolixus.
24418313	9	30	theme	Proteomic	1334:1342	arg1	analysis					1344:1351	Proteomic analysis	1334:1351	Proteomic analysis	1334:1351	Proteomic analysis detected 68 proteins (55 were identified and 13 are hypothetical proteins) using the sequences in the R. prolixus genome (http://www.vectorbase.org).
24418313	3	31	theme	comprehensive	497:509	arg1	characterization					521:536	a comprehensive molecular characterization	495:536	a comprehensive molecular characterization of the major components extracted from the EC of R. prolixus	495:597	We performed a comprehensive molecular characterization of the major components extracted from the EC of R. prolixus, and we discuss the role of the different molecules that were identified during the eclosion process.
24418313	15	32	theme	first	2083:2087	arg1	this					2071:2074	this	2071:2074	this	2071:2074	To our knowledge, this is the first study to describe the global molecular composition of an EC in insects.
24418313	15	32	theme	first	2083:2087	arg1	study					2089:2093	the first study	2079:2093	the first study to describe the global molecular composition of an EC in insects	2079:2158	To our knowledge, this is the first study to describe the global molecular composition of an EC in insects.
24418313	3	33	theme	major	545:549	arg1	components					551:560	the major components	541:560	the major components extracted from the EC of R. prolixus	541:597	We performed a comprehensive molecular characterization of the major components extracted from the EC of R. prolixus, and we discuss the role of the different molecules that were identified during the eclosion process.
24418313	1	34	theme	physical	185:192	arg1	protection					194:203	physical protection	185:203	physical protection	185:203	The embryonic cuticle (EC) of Rhodnius prolixus envelopes the entire body of the embryo during hatching and provides physical protection, allowing the embryo to pass through a narrow chorionic border.
24418313	2	35	theme	molecular	441:449	arg1	composition					451:461	the molecular composition	437:461	the molecular composition of this structure	437:479	Most of the knowledge about the EC of insects is derived from studies on ultrastructure and secretion processes during embryonic development, and little is known about the molecular composition of this structure.
24418313	9	36	dep	proteins	1365:1372	arg1	13					1398:1399	13	1398:1399	13	1398:1399	Proteomic analysis detected 68 proteins (55 were identified and 13 are hypothetical proteins) using the sequences in the R. prolixus genome (http://www.vectorbase.org).
24418313	9	36	dep	proteins	1365:1372	arg1	55					1375:1376	55	1375:1376	55	1375:1376	Proteomic analysis detected 68 proteins (55 were identified and 13 are hypothetical proteins) using the sequences in the R. prolixus genome (http://www.vectorbase.org).
24418313	0	37	dep	cuticle	59:65	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of Rhodnius prolixus	0:46	Molecular characterization of Rhodnius prolixus' embryonic cuticle.
24418313	7	38	dep	neutral	1166:1172	arg1	%					1177:1177	12%	1175:1177	12%	1175:1177	In addition to chitin, we also identified amino (15%), neutral (12%) and acidic (3%) carbohydrates in the EC of R. prolixus.
24418313	2	39	theme	secretion	361:369	arg1	processes					371:379	ultrastructure and secretion processes	342:379	ultrastructure and secretion processes during embryonic development	342:408	Most of the knowledge about the EC of insects is derived from studies on ultrastructure and secretion processes during embryonic development, and little is known about the molecular composition of this structure.
24418313	0	40	theme	prolixus	39:46	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of Rhodnius prolixus	0:46	Molecular characterization of Rhodnius prolixus' embryonic cuticle.
24418313	12	41	theme	total	1700:1704	arg1	proteins					1706:1713	the total proteins	1696:1713	the total proteins identified	1696:1724	Approximately 35% of the total proteins identified were classified as having a structural function.
24418313	9	42	dep	genome	1467:1472	arg1	http					1475:1478	http	1475:1478	http://www.vectorbase.org	1475:1499	Proteomic analysis detected 68 proteins (55 were identified and 13 are hypothetical proteins) using the sequences in the R. prolixus genome (http://www.vectorbase.org).
24418313	9	43	theme	R. prolixus	1455:1465	arg1	genome					1467:1472	the R. prolixus genome	1451:1472	the R. prolixus genome (http://www.vectorbase.org)	1451:1500	Proteomic analysis detected 68 proteins (55 were identified and 13 are hypothetical proteins) using the sequences in the R. prolixus genome (http://www.vectorbase.org).
24418313	11	44	theme	cuticle	1610:1616	arg1	proteins					1583:1590	These proteins	1577:1590	These proteins	1577:1590	These proteins are unequivocally cuticle proteins, and they have been described in other insects.
24418313	11	44	theme	cuticle	1610:1616	arg1	proteins					1618:1625	cuticle proteins	1610:1625	cuticle proteins	1610:1625	These proteins are unequivocally cuticle proteins, and they have been described in other insects.
24418313	4	45	theme	R. prolixus	787:797	arg1	EC					781:782	the EC	777:782	the EC of R. prolixus	777:797	The results showed that, similar to the post-embryonic cuticles of insects, the EC of R. prolixus is primarily composed of carbohydrates (57%), lipids (19%), and proteins (8%).
24418313	13	46	theme	Chitin-binding	1775:1788	arg1	proteins					1884:1891	all proteins	1880:1891	all proteins associated with cuticle metabolism	1880:1926	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	13	46	theme	Chitin-binding	1775:1788	arg1	protein					1790:1796	Chitin-binding protein	1775:1796	Chitin-binding protein	1775:1796	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	13	46	theme	Chitin-binding	1775:1788	arg1	peptidase					1805:1813	amino peptidase	1799:1813	amino peptidase	1799:1813	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	1	47	theme	Rhodnius	98:105	arg1	prolixus					107:114	Rhodnius prolixus	98:114	Rhodnius prolixus	98:114	The embryonic cuticle (EC) of Rhodnius prolixus envelopes the entire body of the embryo during hatching and provides physical protection, allowing the embryo to pass through a narrow chorionic border.
24418313	0	48	theme	embryonic	49:57	arg1	cuticle					59:65	' embryonic cuticle	47:65	' embryonic cuticle	47:65	Molecular characterization of Rhodnius prolixus' embryonic cuticle.
24418313	10	49	theme	cuticle	1557:1563	arg1	metabolism					1565:1574	cuticle metabolism	1557:1574	cuticle metabolism	1557:1574	Among these proteins, 8 out of 15 are associated with cuticle metabolism.
24418313	3	50	theme	molecules	641:649	arg1	role					619:622	the role	615:622	the role of the different molecules that were identified during the eclosion process	615:698	We performed a comprehensive molecular characterization of the major components extracted from the EC of R. prolixus, and we discuss the role of the different molecules that were identified during the eclosion process.
24418313	13	51	theme	acid	1822:1825	arg1	oxidase					1827:1833	amino acid oxidase	1816:1833	amino acid oxidase	1816:1833	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	13	51	theme	acid	1822:1825	arg1	peptidase					1805:1813	amino peptidase	1799:1813	amino peptidase	1799:1813	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	1	52	theme	prolixus	107:114	arg1	EC					91:92	EC	91:92	EC	91:92	The embryonic cuticle (EC) of Rhodnius prolixus envelopes the entire body of the embryo during hatching and provides physical protection, allowing the embryo to pass through a narrow chorionic border.
24418313	1	52	theme	prolixus	107:114	arg1	cuticle					82:88	The embryonic cuticle	68:88	The embryonic cuticle (EC) of Rhodnius prolixus	68:114	The embryonic cuticle (EC) of Rhodnius prolixus envelopes the entire body of the embryo during hatching and provides physical protection, allowing the embryo to pass through a narrow chorionic border.
24418313	5	53	theme	EC	1020:1021	arg1	body					1008:1011	the body	1004:1011	the body of the EC	1004:1021	Considering only the carbohydrates, chitin is by far the major component (approximately 70%), and it is found primarily along the body of the EC.
24418313	5	54	theme	major	935:939	arg1	component					941:949	the major component	931:949	the major component (approximately 70%)	931:969	Considering only the carbohydrates, chitin is by far the major component (approximately 70%), and it is found primarily along the body of the EC.
24418313	5	54	theme	major	935:939	arg1	%					968:968	approximately 70%	952:968	approximately 70%	952:968	Considering only the carbohydrates, chitin is by far the major component (approximately 70%), and it is found primarily along the body of the EC.
24418313	5	54	theme	major	935:939	arg1	chitin					914:919	chitin	914:919	chitin	914:919	Considering only the carbohydrates, chitin is by far the major component (approximately 70%), and it is found primarily along the body of the EC.
24418313	7	55	theme	neutral	1166:1172	arg1	amino					1153:1157	amino	1153:1157	amino (15%)	1153:1163	In addition to chitin, we also identified amino (15%), neutral (12%) and acidic (3%) carbohydrates in the EC of R. prolixus.
24418313	7	55	theme	neutral	1166:1172	arg1	%					1162:1162	15%	1160:1162	15%	1160:1162	In addition to chitin, we also identified amino (15%), neutral (12%) and acidic (3%) carbohydrates in the EC of R. prolixus.
24418313	6	56	from	prolongations	1054:1066	arg1	absent					1040:1045	absent	1040:1045	absent	1040:1045	It is scarce or absent in its prolongations, which are composed of glycosaminoglycans.
24418313	15	57	from	composition	2128:2138	arg1	insects					2152:2158	insects	2152:2158	insects	2152:2158	To our knowledge, this is the first study to describe the global molecular composition of an EC in insects.
24418313	4	58	theme	post-embryonic	741:754	arg1	cuticles					756:763	the post-embryonic cuticles	737:763	the post-embryonic cuticles of insects	737:774	The results showed that, similar to the post-embryonic cuticles of insects, the EC of R. prolixus is primarily composed of carbohydrates (57%), lipids (19%), and proteins (8%).
24418313	14	59	theme	cuticle	1950:1956	arg1	constituents					1958:1969	cuticle constituents	1950:1969	cuticle constituents	1950:1969	Proteins known to be cuticle constituents may be related to the function of the EC in assisting the insect during eclosion.
24418313	2	60	theme	structure	471:479	arg1	composition					451:461	the molecular composition	437:461	the molecular composition of this structure	437:479	Most of the knowledge about the EC of insects is derived from studies on ultrastructure and secretion processes during embryonic development, and little is known about the molecular composition of this structure.
24418313	3	61	theme	different	631:639	arg1	molecules					641:649	the different molecules	627:649	the different molecules that were identified during the eclosion process	627:698	We performed a comprehensive molecular characterization of the major components extracted from the EC of R. prolixus, and we discuss the role of the different molecules that were identified during the eclosion process.
24418313	3	62	theme	R. prolixus	587:597	arg1	EC					581:582	the EC	577:582	the EC of R. prolixus	577:597	We performed a comprehensive molecular characterization of the major components extracted from the EC of R. prolixus, and we discuss the role of the different molecules that were identified during the eclosion process.
24418313	1	63	theme	entire	130:135	arg1	body					137:140	the entire body	126:140	the entire body of the embryo	126:154	The embryonic cuticle (EC) of Rhodnius prolixus envelopes the entire body of the embryo during hatching and provides physical protection, allowing the embryo to pass through a narrow chorionic border.
24418313	13	64	theme	cuticle	1909:1915	arg1	metabolism					1917:1926	cuticle metabolism	1909:1926	cuticle metabolism	1909:1926	Chitin-binding protein, amino peptidase, amino acid oxidase, oxidoreductase, catalase and peroxidase are all proteins associated with cuticle metabolism.
24418313	11	65	theme	other	1660:1664	arg1	insects					1666:1672	other insects	1660:1672	other insects	1660:1672	These proteins are unequivocally cuticle proteins, and they have been described in other insects.
24418313	4	66	theme	insects	768:774	arg1	cuticles					756:763	the post-embryonic cuticles	737:763	the post-embryonic cuticles of insects	737:774	The results showed that, similar to the post-embryonic cuticles of insects, the EC of R. prolixus is primarily composed of carbohydrates (57%), lipids (19%), and proteins (8%).
24418313	15	67	theme	EC	2146:2147	arg1	composition					2128:2138	the global molecular composition	2107:2138	the global molecular composition of an EC in insects	2107:2158	To our knowledge, this is the first study to describe the global molecular composition of an EC in insects.
24418313	6	68	from	absent	1040:1045	arg1	prolongations					1054:1066	its prolongations	1050:1066	its prolongations	1050:1066	It is scarce or absent in its prolongations, which are composed of glycosaminoglycans.
24418313	12	69	theme	proteins	1706:1713	arg1	proteins					1706:1713	the total proteins	1696:1713	the total proteins identified	1696:1724	Approximately 35% of the total proteins identified were classified as having a structural function.
24418313	12	69	theme	proteins	1706:1713	arg1	%					1691:1691	Approximately 35%	1675:1691	Approximately 35% of the total proteins identified	1675:1724	Approximately 35% of the total proteins identified were classified as having a structural function.
24463731	4	0	theme	potassium	541:549	arg1	carbonate					551:559	potassium carbonate	541:559	potassium carbonate	541:559	The glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxing agent.
24463731	4	0	theme	potassium	541:549	arg1	agent					576:580	the fluxing agent	564:580	the fluxing agent	564:580	The glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxing agent.
24463731	4	0	theme	potassium	541:549	arg1	limestone					492:500	limestone	492:500	limestone (calcium and magnesium carbonates)	492:535	The glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxing agent.
24463731	4	0	theme	potassium	541:549	arg1	ash					487:489	ash	487:489	ash	487:489	The glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxing agent.
24463731	4	1	dep	limestone	492:500	arg1	magnesium					515:523	magnesium	515:523	magnesium	515:523	The glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxing agent.
24463731	4	1	dep	limestone	492:500	arg1	calcium					503:509	calcium	503:509	calcium	503:509	The glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxing agent.
24463731	3	2	theme	bagasse	350:356	arg1	SCBA					363:366	SCBA	363:366	SCBA	363:366	In this work we report on the use of sugarcane bagasse ash (SCBA) to produce glass-ceramics with silicates as the major crystalline phases.
24463731	3	2	theme	bagasse	350:356	arg1	ash					358:360	sugarcane bagasse ash	340:360	sugarcane bagasse ash (SCBA)	340:367	In this work we report on the use of sugarcane bagasse ash (SCBA) to produce glass-ceramics with silicates as the major crystalline phases.
24463731	3	3	theme	ash	358:360	arg1	use					333:335	the use	329:335	the use of sugarcane bagasse ash (SCBA)	329:367	In this work we report on the use of sugarcane bagasse ash (SCBA) to produce glass-ceramics with silicates as the major crystalline phases.
24463731	5	4	theme	chemical	628:635	arg1	composition					637:647	the chemical composition	624:647	the chemical composition of the glasses	624:662	X-ray fluorescence was used to determine the chemical composition of the glasses and their crystallization was assessed by using thermal analysis (DTA/DSC/TGA) and X-ray diffraction.
24463731	3	5	with	glass-ceramics	380:393	arg1	silicates					400:408	silicates	400:408	silicates	400:408	In this work we report on the use of sugarcane bagasse ash (SCBA) to produce glass-ceramics with silicates as the major crystalline phases.
24463731	5	6	theme	X-ray	583:587	arg1	fluorescence					589:600	X-ray fluorescence	583:600	X-ray fluorescence	583:600	X-ray fluorescence was used to determine the chemical composition of the glasses and their crystallization was assessed by using thermal analysis (DTA/DSC/TGA) and X-ray diffraction.
24463731	1	7	from	important	144:152	arg1	industries					186:195	the ceramic and construction industries	157:195	the ceramic and construction industries	157:195	Some aluminosilicates, for example mullite and wollastonite, are very important in the ceramic and construction industries.
24463731	2	8	theme	crystal	288:294	arg1	phase					296:300	the main crystal phase	279:300	the main crystal phase	279:300	The most significant glass-ceramic for building applications has wollastonite as the main crystal phase.
24463731	2	8	theme	crystal	288:294	arg1	wollastonite					263:274	wollastonite	263:274	wollastonite	263:274	The most significant glass-ceramic for building applications has wollastonite as the main crystal phase.
24463731	1	9	theme	ceramic	161:167	arg1	industries					186:195	the ceramic and construction industries	157:195	the ceramic and construction industries	157:195	Some aluminosilicates, for example mullite and wollastonite, are very important in the ceramic and construction industries.
24463731	3	10	theme	sugarcane	340:348	arg1	SCBA					363:366	SCBA	363:366	SCBA	363:366	In this work we report on the use of sugarcane bagasse ash (SCBA) to produce glass-ceramics with silicates as the major crystalline phases.
24463731	3	10	theme	sugarcane	340:348	arg1	ash					358:360	sugarcane bagasse ash	340:360	sugarcane bagasse ash (SCBA)	340:367	In this work we report on the use of sugarcane bagasse ash (SCBA) to produce glass-ceramics with silicates as the major crystalline phases.
24463731	6	11	theme	major	854:858	arg1	phase					860:864	the major phase	850:864	the major phase	850:864	The results showed that glass-ceramic material can be produced with wollastonite as the major phase, at a temperature lower than 900 °C.
24463731	6	11	theme	major	854:858	arg1	material					804:811	glass-ceramic material	790:811	glass-ceramic material	790:811	The results showed that glass-ceramic material can be produced with wollastonite as the major phase, at a temperature lower than 900 °C.
24463731	2	12	contain	has	259:261	arg1	glass-ceramic					219:231	The most significant glass-ceramic	198:231	The most significant glass-ceramic for building applications	198:257	The most significant glass-ceramic for building applications has wollastonite as the main crystal phase.
24463731	2	12	contain	has	259:261	arg2	wollastonite					263:274	wollastonite	263:274	wollastonite	263:274	The most significant glass-ceramic for building applications has wollastonite as the main crystal phase.
24463731	2	12	contain	has	259:261	arg2	phase					296:300	the main crystal phase	279:300	the main crystal phase	279:300	The most significant glass-ceramic for building applications has wollastonite as the main crystal phase.
24463731	2	13	theme	main	283:286	arg1	phase					296:300	the main crystal phase	279:300	the main crystal phase	279:300	The most significant glass-ceramic for building applications has wollastonite as the main crystal phase.
24463731	2	13	theme	main	283:286	arg1	wollastonite					263:274	wollastonite	263:274	wollastonite	263:274	The most significant glass-ceramic for building applications has wollastonite as the main crystal phase.
24463731	3	14	theme	crystalline	423:433	arg1	glass-ceramics					380:393	glass-ceramics	380:393	glass-ceramics with silicates	380:408	In this work we report on the use of sugarcane bagasse ash (SCBA) to produce glass-ceramics with silicates as the major crystalline phases.
24463731	3	14	theme	crystalline	423:433	arg1	phases					435:440	the major crystalline phases	413:440	the major crystalline phases	413:440	In this work we report on the use of sugarcane bagasse ash (SCBA) to produce glass-ceramics with silicates as the major crystalline phases.
24463731	1	15	theme	construction	173:184	arg1	industries					186:195	the ceramic and construction industries	157:195	the ceramic and construction industries	157:195	Some aluminosilicates, for example mullite and wollastonite, are very important in the ceramic and construction industries.
24463731	0	16	theme	bagasse	26:32	arg1	Valorization					0:11	Valorization	0:11	Valorization of sugarcane bagasse	0:32	Valorization of sugarcane bagasse ash: producing glass-ceramic materials.
24463731	0	17	theme	sugarcane	16:24	arg1	bagasse					26:32	sugarcane bagasse	16:32	sugarcane bagasse	16:32	Valorization of sugarcane bagasse ash: producing glass-ceramic materials.
24463731	3	18	theme	major	417:421	arg1	glass-ceramics					380:393	glass-ceramics	380:393	glass-ceramics with silicates	380:408	In this work we report on the use of sugarcane bagasse ash (SCBA) to produce glass-ceramics with silicates as the major crystalline phases.
24463731	3	18	theme	major	417:421	arg1	phases					435:440	the major crystalline phases	413:440	the major crystalline phases	413:440	In this work we report on the use of sugarcane bagasse ash (SCBA) to produce glass-ceramics with silicates as the major crystalline phases.
24463731	5	19	theme	X-ray	747:751	arg1	diffraction					753:763	X-ray diffraction	747:763	X-ray diffraction	747:763	X-ray fluorescence was used to determine the chemical composition of the glasses and their crystallization was assessed by using thermal analysis (DTA/DSC/TGA) and X-ray diffraction.
24463731	2	20	theme	building	237:244	arg1	applications					246:257	building applications	237:257	building applications	237:257	The most significant glass-ceramic for building applications has wollastonite as the main crystal phase.
24463731	5	21	theme	thermal	712:718	arg1	DTA/DSC/TGA					730:740	DTA/DSC/TGA	730:740	DTA/DSC/TGA	730:740	X-ray fluorescence was used to determine the chemical composition of the glasses and their crystallization was assessed by using thermal analysis (DTA/DSC/TGA) and X-ray diffraction.
24463731	5	21	theme	thermal	712:718	arg1	analysis					720:727	thermal analysis	712:727	thermal analysis (DTA/DSC/TGA)	712:741	X-ray fluorescence was used to determine the chemical composition of the glasses and their crystallization was assessed by using thermal analysis (DTA/DSC/TGA) and X-ray diffraction.
24463731	0	22	theme	glass-ceramic	49:61	arg1	materials					63:71	glass-ceramic materials	49:71	glass-ceramic materials	49:71	Valorization of sugarcane bagasse ash: producing glass-ceramic materials.
24463731	6	23	theme	lower	884:888	arg1	temperature					872:882	a temperature	870:882	a temperature lower than 900 °C	870:900	The results showed that glass-ceramic material can be produced with wollastonite as the major phase, at a temperature lower than 900 °C.
24463731	2	24	theme	significant	207:217	arg1	glass-ceramic					219:231	The most significant glass-ceramic	198:231	The most significant glass-ceramic for building applications	198:257	The most significant glass-ceramic for building applications has wollastonite as the main crystal phase.
24463731	4	25	theme	fluxing	568:574	arg1	carbonate					551:559	potassium carbonate	541:559	potassium carbonate	541:559	The glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxing agent.
24463731	4	25	theme	fluxing	568:574	arg1	agent					576:580	the fluxing agent	564:580	the fluxing agent	564:580	The glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxing agent.
24463731	4	25	theme	fluxing	568:574	arg1	limestone					492:500	limestone	492:500	limestone (calcium and magnesium carbonates)	492:535	The glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxing agent.
24463731	4	25	theme	fluxing	568:574	arg1	ash					487:489	ash	487:489	ash	487:489	The glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxing agent.
24463731	5	26	theme	glasses	656:662	arg1	composition					637:647	the chemical composition	624:647	the chemical composition of the glasses	624:662	X-ray fluorescence was used to determine the chemical composition of the glasses and their crystallization was assessed by using thermal analysis (DTA/DSC/TGA) and X-ray diffraction.
24463731	5	27	used	used	606:609	arg2	fluorescence					589:600	X-ray fluorescence	583:600	X-ray fluorescence	583:600	X-ray fluorescence was used to determine the chemical composition of the glasses and their crystallization was assessed by using thermal analysis (DTA/DSC/TGA) and X-ray diffraction.
24463731	6	28	theme	glass-ceramic	790:802	arg1	phase					860:864	the major phase	850:864	the major phase	850:864	The results showed that glass-ceramic material can be produced with wollastonite as the major phase, at a temperature lower than 900 °C.
24463731	6	28	theme	glass-ceramic	790:802	arg1	material					804:811	glass-ceramic material	790:811	glass-ceramic material	790:811	The results showed that glass-ceramic material can be produced with wollastonite as the major phase, at a temperature lower than 900 °C.
24463731	1	29	from	industries	186:195	arg1	important					144:152	important	144:152	important	144:152	Some aluminosilicates, for example mullite and wollastonite, are very important in the ceramic and construction industries.
24463731	1	30	dep	example	101:107	arg1	wollastonite					121:132	wollastonite	121:132	wollastonite	121:132	Some aluminosilicates, for example mullite and wollastonite, are very important in the ceramic and construction industries.
24463731	1	30	dep	example	101:107	arg1	mullite					109:115	mullite	109:115	mullite	109:115	Some aluminosilicates, for example mullite and wollastonite, are very important in the ceramic and construction industries.
24463731	4	31	dep	calcium	503:509	arg1	carbonates					525:534	carbonates	525:534	carbonates	525:534	The glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxing agent.
23720392	0	0	theme	engineering	95:105	arg1	applications					107:118	bone tissue engineering applications	83:118	bone tissue engineering applications	83:118	Carboxymethyl cellulose-hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications.
23720392	6	1	theme	flight	999:1004	arg1	ToF-SIMS					1039:1046	ToF-SIMS	1039:1046	ToF-SIMS	1039:1046	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	6	1	theme	flight	999:1004	arg1	spectrometry					1025:1036	flight secondary ion mass spectrometry	999:1036	flight secondary ion mass spectrometry (ToF-SIMS)	999:1047	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	0	2	theme	tissue	88:93	arg1	applications					107:118	bone tissue engineering applications	83:118	bone tissue engineering applications	83:118	Carboxymethyl cellulose-hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications.
23720392	3	3	theme	hydrogel	511:518	arg1	matrix					520:525	the hydrogel matrix	507:525	the hydrogel matrix	507:525	The strategy for inserting HA nanocrystals within the hydrogel matrix consists of making the freeze-dried hydrogel to swell in a solution containing HA microcrystals.
23720392	1	4	theme	hierarchical	276:287	arg1	architecture					289:300	hierarchical architecture	276:300	hierarchical architecture over several length scales	276:327	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	8	5	theme	matrix	1411:1416	arg1	production					1371:1380	the production	1367:1380	the production of mineralized extracellular matrix	1367:1416	The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix.
23720392	7	6	theme	CMC-HA	1090:1095	arg1	hydrogel					1097:1104	CMC-HA hydrogel	1090:1104	CMC-HA hydrogel	1090:1104	The biological performance of CMC-HA hydrogel were tested by using osteoblast MG63 line and compared with a CMC-based hydrogel without HA.
23720392	7	7	theme	hydrogel	1097:1104	arg1	performance					1075:1085	The biological performance	1060:1085	The biological performance of CMC-HA hydrogel	1060:1104	The biological performance of CMC-HA hydrogel were tested by using osteoblast MG63 line and compared with a CMC-based hydrogel without HA.
23720392	3	8	theme	freeze-dried	550:561	arg1	hydrogel					563:570	the freeze-dried hydrogel	546:570	the freeze-dried hydrogel to swell	546:579	The strategy for inserting HA nanocrystals within the hydrogel matrix consists of making the freeze-dried hydrogel to swell in a solution containing HA microcrystals.
23720392	3	9	contain	containing	595:604	arg1	solution					586:593	a solution	584:593	a solution containing HA microcrystals	584:621	The strategy for inserting HA nanocrystals within the hydrogel matrix consists of making the freeze-dried hydrogel to swell in a solution containing HA microcrystals.
23720392	3	9	contain	containing	595:604	arg2	microcrystals					609:621	HA microcrystals	606:621	HA microcrystals	606:621	The strategy for inserting HA nanocrystals within the hydrogel matrix consists of making the freeze-dried hydrogel to swell in a solution containing HA microcrystals.
23720392	8	10	theme	gene	1240:1243	arg1	expression					1245:1254	gene expression	1240:1254	gene expression	1240:1254	The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix.
23720392	4	11	theme	field	793:797	arg1	FESEM					838:842	FESEM	838:842	FESEM	838:842	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	4	11	theme	field	793:797	arg1	microscopy					826:835	field emission scanning electron microscopy	793:835	field emission scanning electron microscopy (FESEM)	793:843	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	8	12	theme	osteoblast	1217:1226	arg1	markers					1228:1234	osteoblast markers	1217:1234	osteoblast markers	1217:1234	The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix.
23720392	7	13	theme	CMC-based	1168:1176	arg1	hydrogel					1178:1185	a CMC-based hydrogel	1166:1185	a CMC-based hydrogel without HA	1166:1196	The biological performance of CMC-HA hydrogel were tested by using osteoblast MG63 line and compared with a CMC-based hydrogel without HA.
23720392	1	14	theme	hydroxyapatite	198:211	arg1	nanocrystals					218:229	hydroxyapatite (HA) nanocrystals	198:229	hydroxyapatite (HA) nanocrystals	198:229	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	8	15	theme	HA	1284:1285	arg1	addition					1272:1279	the addition	1268:1279	the addition of HA to CMC hydrogel	1268:1301	The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix.
23720392	6	16	from	distribution	907:918	arg1	surface					941:947	the surface	937:947	the surface	937:947	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	4	17	theme	physicochemical	684:698	arg1	point					718:722	a physicochemical and morphological point	682:722	a physicochemical and morphological point of view	682:730	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	2	18	theme	-based	440:445	arg1	hydrogel					447:454	a carboxymethylcellulose (CMC)-based hydrogel	410:454	a carboxymethylcellulose (CMC)-based hydrogel	410:454	In this work, we reported a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel.
23720392	1	19	theme	several	307:313	arg1	scales					322:327	several length scales	307:327	several length scales	307:327	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	7	20	theme	biological	1064:1073	arg1	performance					1075:1085	The biological performance	1060:1085	The biological performance of CMC-HA hydrogel	1060:1104	The biological performance of CMC-HA hydrogel were tested by using osteoblast MG63 line and compared with a CMC-based hydrogel without HA.
23720392	4	21	theme	FTIR	744:747	arg1	spectroscopy					749:760	FTIR spectroscopy	744:760	FTIR spectroscopy	744:760	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	8	22	theme	markers	1228:1234	arg1	evaluation					1203:1212	The evaluation	1199:1212	The evaluation of osteoblast markers and gene expression	1199:1254	The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix.
23720392	1	23	theme	length	315:320	arg1	scales					322:327	several length scales	307:327	several length scales	307:327	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	1	24	theme	Natural	121:127	arg1	material					179:186	a complex inorganic-organic nanocomposite material	137:186	a complex inorganic-organic nanocomposite material	137:186	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	1	24	theme	Natural	121:127	arg1	bone					129:132	Natural bone	121:132	Natural bone	121:132	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	5	25	theme	No	846:847	arg1	release					849:855	No release	846:855	No release of HA	846:861	No release of HA was measured in water or NaCl solution.
23720392	0	26	theme	hybrid	39:44	arg1	hydrogel					46:53	hybrid hydrogel	39:53	hybrid hydrogel	39:53	Carboxymethyl cellulose-hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications.
23720392	0	26	theme	hybrid	39:44	arg1	material					70:77	a composite material	58:77	a composite material for bone tissue engineering applications	58:118	Carboxymethyl cellulose-hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications.
23720392	8	27	theme	metabolic	1335:1343	arg1	activity					1345:1352	metabolic activity	1335:1352	metabolic activity	1335:1352	The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix.
23720392	2	28	theme	hybrid	362:367	arg1	material					369:376	a new hybrid material	356:376	a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel	356:454	In this work, we reported a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel.
23720392	2	28	theme	hybrid	362:367	arg1	CMC-HA					379:384	CMC-HA	379:384	CMC-HA	379:384	In this work, we reported a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel.
23720392	4	29	theme	CMC-HA	638:643	arg1	hydrogel					645:652	The composite CMC-HA hydrogel	624:652	The composite CMC-HA hydrogel	624:652	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	7	30	theme	osteoblast	1127:1136	arg1	line					1143:1146	osteoblast MG63 line	1127:1146	osteoblast MG63 line	1127:1146	The biological performance of CMC-HA hydrogel were tested by using osteoblast MG63 line and compared with a CMC-based hydrogel without HA.
23720392	2	31	contain	containing	387:396	arg1	material					369:376	a new hybrid material	356:376	a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel	356:454	In this work, we reported a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel.
23720392	2	31	contain	containing	387:396	arg1	CMC-HA					379:384	CMC-HA	379:384	CMC-HA	379:384	In this work, we reported a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel.
23720392	2	31	contain	containing	387:396	arg2	HA					398:399	HA	398:399	HA drown in a carboxymethylcellulose (CMC)-based hydrogel	398:454	In this work, we reported a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel.
23720392	8	32	theme	CMC	1290:1292	arg1	hydrogel					1294:1301	CMC hydrogel	1290:1301	CMC hydrogel	1290:1301	The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix.
23720392	6	33	theme	spectrometry	1025:1036	arg1	time					991:994	time	991:994	time of flight secondary ion mass spectrometry (ToF-SIMS)	991:1047	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	6	33	theme	spectrometry	1025:1036	arg1	FESEM					1053:1057	FESEM	1053:1057	FESEM	1053:1057	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	4	34	theme	morphological	704:716	arg1	point					718:722	a physicochemical and morphological point	682:722	a physicochemical and morphological point of view	682:730	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	3	35	theme	HA	606:607	arg1	microcrystals					609:621	HA microcrystals	606:621	HA microcrystals	606:621	The strategy for inserting HA nanocrystals within the hydrogel matrix consists of making the freeze-dried hydrogel to swell in a solution containing HA microcrystals.
23720392	1	36	theme	collagen	235:242	arg1	fibrils					244:250	collagen fibrils	235:250	collagen fibrils	235:250	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	6	37	theme	mass	1020:1023	arg1	ToF-SIMS					1039:1046	ToF-SIMS	1039:1046	ToF-SIMS	1039:1046	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	6	37	theme	mass	1020:1023	arg1	spectrometry					1025:1036	flight secondary ion mass spectrometry	999:1036	flight secondary ion mass spectrometry (ToF-SIMS)	999:1047	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	8	38	theme	extracellular	1397:1409	arg1	matrix					1411:1416	mineralized extracellular matrix	1385:1416	mineralized extracellular matrix	1385:1416	The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix.
23720392	2	39	theme	new	358:360	arg1	material					369:376	a new hybrid material	356:376	a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel	356:454	In this work, we reported a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel.
23720392	2	39	theme	new	358:360	arg1	CMC-HA					379:384	CMC-HA	379:384	CMC-HA	379:384	In this work, we reported a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel.
23720392	0	40	theme	composite	60:68	arg1	hydrogel					46:53	hybrid hydrogel	39:53	hybrid hydrogel	39:53	Carboxymethyl cellulose-hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications.
23720392	0	40	theme	composite	60:68	arg1	material					70:77	a composite material	58:77	a composite material for bone tissue engineering applications	58:118	Carboxymethyl cellulose-hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications.
23720392	8	41	theme	mineralized	1385:1395	arg1	matrix					1411:1416	mineralized extracellular matrix	1385:1416	mineralized extracellular matrix	1385:1416	The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix.
23720392	4	42	theme	view	727:730	arg1	point					718:722	a physicochemical and morphological point	682:722	a physicochemical and morphological point of view	682:730	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	6	43	theme	crystal	926:932	arg1	distribution					907:918	The distribution	903:918	The distribution of HA crystal on the surface and inside the hydrogel	903:971	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	4	44	theme	scanning	808:815	arg1	FESEM					838:842	FESEM	838:842	FESEM	838:842	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	4	44	theme	scanning	808:815	arg1	microscopy					826:835	field emission scanning electron microscopy	793:835	field emission scanning electron microscopy (FESEM)	793:843	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	1	45	theme	complex	139:145	arg1	material					179:186	a complex inorganic-organic nanocomposite material	137:186	a complex inorganic-organic nanocomposite material	137:186	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	1	45	theme	complex	139:145	arg1	bone					129:132	Natural bone	121:132	Natural bone	121:132	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	4	46	theme	rheological	763:773	arg1	measurements					775:786	rheological measurements	763:786	rheological measurements	763:786	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	6	47	theme	ion	1016:1018	arg1	ToF-SIMS					1039:1046	ToF-SIMS	1039:1046	ToF-SIMS	1039:1046	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	6	47	theme	ion	1016:1018	arg1	spectrometry					1025:1036	flight secondary ion mass spectrometry	999:1036	flight secondary ion mass spectrometry (ToF-SIMS)	999:1047	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	4	48	theme	emission	799:806	arg1	FESEM					838:842	FESEM	838:842	FESEM	838:842	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	4	48	theme	emission	799:806	arg1	microscopy					826:835	field emission scanning electron microscopy	793:835	field emission scanning electron microscopy (FESEM)	793:843	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	1	49	theme	inorganic-organic	147:163	arg1	material					179:186	a complex inorganic-organic nanocomposite material	137:186	a complex inorganic-organic nanocomposite material	137:186	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	1	49	theme	inorganic-organic	147:163	arg1	bone					129:132	Natural bone	121:132	Natural bone	121:132	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	8	50	theme	cell	1312:1315	arg1	proliferation					1317:1329	cell proliferation	1312:1329	cell proliferation	1312:1329	The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix.
23720392	6	51	theme	secondary	1006:1014	arg1	ToF-SIMS					1039:1046	ToF-SIMS	1039:1046	ToF-SIMS	1039:1046	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	6	51	theme	secondary	1006:1014	arg1	spectrometry					1025:1036	flight secondary ion mass spectrometry	999:1036	flight secondary ion mass spectrometry (ToF-SIMS)	999:1047	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	0	52	theme	bone	83:86	arg1	applications					107:118	bone tissue engineering applications	83:118	bone tissue engineering applications	83:118	Carboxymethyl cellulose-hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications.
23720392	5	53	theme	HA	860:861	arg1	release					849:855	No release	846:855	No release of HA	846:861	No release of HA was measured in water or NaCl solution.
23720392	8	54	theme	expression	1245:1254	arg1	evaluation					1203:1212	The evaluation	1199:1212	The evaluation of osteoblast markers and gene expression	1199:1254	The evaluation of osteoblast markers and gene expression showed that the addition of HA to CMC hydrogel enhanced cell proliferation and metabolic activity and promoted the production of mineralized extracellular matrix.
23720392	1	55	theme	nanocomposite	165:177	arg1	material					179:186	a complex inorganic-organic nanocomposite material	137:186	a complex inorganic-organic nanocomposite material	137:186	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	1	55	theme	nanocomposite	165:177	arg1	bone					129:132	Natural bone	121:132	Natural bone	121:132	Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales.
23720392	3	56	theme	HA	484:485	arg1	nanocrystals					487:498	HA nanocrystals	484:498	HA nanocrystals within the hydrogel matrix	484:525	The strategy for inserting HA nanocrystals within the hydrogel matrix consists of making the freeze-dried hydrogel to swell in a solution containing HA microcrystals.
23720392	4	57	theme	composite	628:636	arg1	hydrogel					645:652	The composite CMC-HA hydrogel	624:652	The composite CMC-HA hydrogel	624:652	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	6	58	theme	HA	923:924	arg1	crystal					926:932	HA crystal	923:932	HA crystal	923:932	The distribution of HA crystal on the surface and inside the hydrogel was determined by time of flight secondary ion mass spectrometry (ToF-SIMS) and FESEM.
23720392	4	59	theme	electron	817:824	arg1	FESEM					838:842	FESEM	838:842	FESEM	838:842	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	4	59	theme	electron	817:824	arg1	microscopy					826:835	field emission scanning electron microscopy	793:835	field emission scanning electron microscopy (FESEM)	793:843	The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM).
23720392	5	60	theme	NaCl	888:891	arg1	solution					893:900	NaCl solution	888:900	NaCl solution	888:900	No release of HA was measured in water or NaCl solution.
26671207	3	0	theme	polidocanol	319:329	arg1	foam					337:340	polidocanol (POL) foam	319:340	polidocanol (POL) foam	319:340	OBJECTIVE To evaluate the stability of polidocanol (POL) foam with the addition of hyaluronic acid (HA).
26671207	4	1	theme	POL	427:429	arg1	mL					418:419	2 mL	416:419	2 mL of 1% POL	416:429	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	1	2	theme	varicose	153:160	arg1	veins					162:166	varicose veins	153:166	varicose veins	153:166	BACKGROUND Foam sclerotherapy is an effective treatment strategy for varicose veins and venous malformations.
26671207	0	3	theme	Foam	68:71	arg1	Stability					73:81	Polidocanol Foam Stability	56:81	Polidocanol Foam Stability	56:81	An Investigation on the Influence of Hyaluronic Acid on Polidocanol Foam Stability.
26671207	0	4	from	Investigation	3:15	arg1	Influence					24:32	the Influence	20:32	the Influence of Hyaluronic Acid on Polidocanol Foam Stability	20:81	An Investigation on the Influence of Hyaluronic Acid on Polidocanol Foam Stability.
26671207	5	5	theme	foam	607:610	arg1	generation					612:621	foam generation	607:621	foam generation	607:621	Tessari's method was used for foam generation.
26671207	8	6	theme	mL	932:933	arg1	addition					915:922	the addition	911:922	the addition of 0.05 mL of 1% HA	911:942	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	4	7	theme	Group	462:466	arg1	B					468:468	Group B	462:468	Group B	462:468	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	8	8	with	seconds	898:904	arg1	addition					915:922	the addition	911:922	the addition of 0.05 mL of 1% HA	911:942	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	8	8	with	seconds	898:904	arg1	addition					981:988	the addition	977:988	the addition of 0.1 mL of 1% HA	977:1007	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	4	9	theme	%	538:538	arg1	POL					540:542	1% POL	537:542	1% POL	537:542	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	4	10	theme	%	425:425	arg1	POL					427:429	1% POL	424:429	1% POL	424:429	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	8	11	theme	1	938:938	arg1	%					939:939	%	939:939	%	939:939	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	4	12	theme	1	537:537	arg1	%					538:538	%	538:538	%	538:538	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	4	13	theme	Group	520:524	arg1	C					526:526	Group C	520:526	Group C	520:526	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	3	14	theme	foam	337:340	arg1	stability					306:314	the stability	302:314	the stability of polidocanol (POL) foam	302:340	OBJECTIVE To evaluate the stability of polidocanol (POL) foam with the addition of hyaluronic acid (HA).
26671207	4	15	theme	1	424:424	arg1	%					425:425	%	425:425	%	425:425	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	6	16	used	used	722:725	arg2	half-life					628:636	The half-life	624:636	The half-life	624:636	The half-life, or the time for a volume of foam to be reduced to half of its original volume, was used to evaluate foam stability.
26671207	6	16	used	used	722:725	arg2	time					646:649	the time	642:649	the time for a volume of foam to be reduced to half of its original volume	642:715	The half-life, or the time for a volume of foam to be reduced to half of its original volume, was used to evaluate foam stability.
26671207	9	17	theme	small	1087:1091	arg1	HA					1104:1105	HA	1104:1105	HA	1104:1105	CONCLUSION The stability of POL foam was highly increased by the addition of small amounts of HA.
26671207	9	17	theme	small	1087:1091	arg1	amounts					1093:1099	small amounts	1087:1099	small amounts of HA	1087:1105	CONCLUSION The stability of POL foam was highly increased by the addition of small amounts of HA.
26671207	4	18	theme	1	499:499	arg1	%					500:500	%	500:500	%	500:500	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	4	19	theme	POL	540:542	arg1	mL					531:532	2 mL	529:532	2 mL of 1% POL	529:542	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	1	20	theme	venous	172:177	arg1	malformations					179:191	venous malformations	172:191	venous malformations	172:191	BACKGROUND Foam sclerotherapy is an effective treatment strategy for varicose veins and venous malformations.
26671207	3	21	theme	acid	374:377	arg1	addition					351:358	the addition	347:358	the addition of hyaluronic acid (HA)	347:382	OBJECTIVE To evaluate the stability of polidocanol (POL) foam with the addition of hyaluronic acid (HA).
26671207	0	22	from	Influence	24:32	arg1	Stability					73:81	Polidocanol Foam Stability	56:81	Polidocanol Foam Stability	56:81	An Investigation on the Influence of Hyaluronic Acid on Polidocanol Foam Stability.
26671207	8	23	dep	RESULTS	797:803	arg1	seconds					898:904	310.6 (±7.53) seconds	884:904	310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA	884:942	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	8	23	dep	RESULTS	797:803	arg1	half-life					809:817	The half-life	805:817	The half-life	805:817	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	8	23	dep	RESULTS	797:803	arg1	seconds					837:843	142.8 (±4.32) seconds	823:843	142.8 (±4.32) seconds for 1% POL without the addition of HA	823:881	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	8	23	dep	RESULTS	797:803	arg1	seconds					964:970	390.4 (±13.06) seconds	949:970	390.4 (±13.06) seconds	949:970	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	0	24	theme	Polidocanol	56:66	arg1	Stability					73:81	Polidocanol Foam Stability	56:81	Polidocanol Foam Stability	56:81	An Investigation on the Influence of Hyaluronic Acid on Polidocanol Foam Stability.
26671207	4	25	theme	HA	502:503	arg1	mL					493:494	0.05 mL	488:494	0.05 mL of 1% HA	488:503	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	4	26	theme	1	441:441	arg1	%					442:442	%	442:442	%	442:442	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	6	27	theme	foam	667:670	arg1	volume					657:662	a volume	655:662	a volume of foam	655:670	The half-life, or the time for a volume of foam to be reduced to half of its original volume, was used to evaluate foam stability.
26671207	4	28	theme	Group	407:411	arg1	A					413:413	Group A	407:413	Group A	407:413	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	1	29	theme	BACKGROUND	84:93	arg1	strategy					140:147	an effective treatment strategy	117:147	an effective treatment strategy for varicose veins and venous malformations	117:191	BACKGROUND Foam sclerotherapy is an effective treatment strategy for varicose veins and venous malformations.
26671207	1	29	theme	BACKGROUND	84:93	arg1	sclerotherapy					100:112	BACKGROUND Foam sclerotherapy	84:112	BACKGROUND Foam sclerotherapy	84:112	BACKGROUND Foam sclerotherapy is an effective treatment strategy for varicose veins and venous malformations.
26671207	4	30	theme	%	500:500	arg1	HA					502:503	1% HA	499:503	1% HA	499:503	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	8	31	theme	1	849:849	arg1	%					850:850	%	850:850	%	850:850	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	8	32	theme	mL	997:998	arg1	addition					981:988	the addition	977:988	the addition of 0.1 mL of 1% HA	977:1007	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	8	33	theme	%	1004:1004	arg1	HA					1006:1007	1% HA	1003:1007	1% HA	1003:1007	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	1	34	theme	Foam	95:98	arg1	strategy					140:147	an effective treatment strategy	117:147	an effective treatment strategy for varicose veins and venous malformations	117:191	BACKGROUND Foam sclerotherapy is an effective treatment strategy for varicose veins and venous malformations.
26671207	1	34	theme	Foam	95:98	arg1	sclerotherapy					100:112	BACKGROUND Foam sclerotherapy	84:112	BACKGROUND Foam sclerotherapy	84:112	BACKGROUND Foam sclerotherapy is an effective treatment strategy for varicose veins and venous malformations.
26671207	3	35	theme	hyaluronic	363:372	arg1	acid					374:377	hyaluronic acid	363:377	hyaluronic acid (HA)	363:382	OBJECTIVE To evaluate the stability of polidocanol (POL) foam with the addition of hyaluronic acid (HA).
26671207	3	35	theme	hyaluronic	363:372	arg1	HA					380:381	HA	380:381	HA	380:381	OBJECTIVE To evaluate the stability of polidocanol (POL) foam with the addition of hyaluronic acid (HA).
26671207	8	36	with	seconds	964:970	arg1	addition					915:922	the addition	911:922	the addition of 0.05 mL of 1% HA	911:942	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	8	36	with	seconds	964:970	arg1	addition					981:988	the addition	977:988	the addition of 0.1 mL of 1% HA	977:1007	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	8	37	theme	1	1003:1003	arg1	%					1004:1004	%	1004:1004	%	1004:1004	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	9	38	theme	HA	1104:1105	arg1	HA					1104:1105	HA	1104:1105	HA	1104:1105	CONCLUSION The stability of POL foam was highly increased by the addition of small amounts of HA.
26671207	9	38	theme	HA	1104:1105	arg1	amounts					1093:1099	small amounts	1087:1099	small amounts of HA	1087:1105	CONCLUSION The stability of POL foam was highly increased by the addition of small amounts of HA.
26671207	4	39	theme	POL	482:484	arg1	mL					473:474	2 mL	471:474	2 mL of 1% POL	471:484	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	4	40	theme	air	457:459	arg1	mL					435:436	0 mL	433:436	0 mL of 1% HA	433:445	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	4	41	theme	HA	559:560	arg1	mL					435:436	0 mL	433:436	0 mL of 1% HA	433:445	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	8	42	theme	HA	880:881	arg1	addition					868:875	the addition	864:875	the addition of HA	864:881	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	8	43	theme	%	850:850	arg1	POL					852:854	1% POL	849:854	1% POL without the addition of HA	849:881	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	4	44	theme	HA	444:445	arg1	mL					435:436	0 mL	433:436	0 mL of 1% HA	433:445	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	8	45	theme	HA	1006:1007	arg1	mL					997:998	0.1 mL	993:998	0.1 mL of 1% HA	993:1007	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	4	46	theme	%	557:557	arg1	HA					559:560	1% HA	556:560	1% HA	556:560	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	6	47	theme	volume	710:715	arg1	half					689:692	half	689:692	half of its original volume	689:715	The half-life, or the time for a volume of foam to be reduced to half of its original volume, was used to evaluate foam stability.
26671207	0	48	theme	Acid	48:51	arg1	Influence					24:32	the Influence	20:32	the Influence of Hyaluronic Acid on Polidocanol Foam Stability	20:81	An Investigation on the Influence of Hyaluronic Acid on Polidocanol Foam Stability.
26671207	4	49	theme	%	442:442	arg1	HA					444:445	1% HA	441:445	1% HA	441:445	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	6	50	dep	time	646:649	arg1	reduced					678:684	reduced	678:684	to be reduced to half of its original volume	672:715	The half-life, or the time for a volume of foam to be reduced to half of its original volume, was used to evaluate foam stability.
26671207	2	51	theme	foam	229:232	arg1	composition					234:244	foam composition	229:244	foam composition	229:244	Foam stability varies according to foam composition, volume, and injection technique.
26671207	4	52	theme	air	572:574	arg1	mL					435:436	0 mL	433:436	0 mL of 1% HA	433:445	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	0	53	theme	Hyaluronic	37:46	arg1	Acid					48:51	Hyaluronic Acid	37:51	Hyaluronic Acid	37:51	An Investigation on the Influence of Hyaluronic Acid on Polidocanol Foam Stability.
26671207	5	54	used	used	598:601	arg2	method					587:592	Tessari's method	577:592	Tessari's method	577:592	Tessari's method was used for foam generation.
26671207	8	55	with	seconds	837:843	arg1	addition					915:922	the addition	911:922	the addition of 0.05 mL of 1% HA	911:942	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	8	55	with	seconds	837:843	arg1	addition					981:988	the addition	977:988	the addition of 0.1 mL of 1% HA	977:1007	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	4	56	theme	air	515:517	arg1	mL					493:494	0.05 mL	488:494	0.05 mL of 1% HA	488:503	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	2	57	theme	injection	259:267	arg1	technique					269:277	injection technique	259:277	injection technique	259:277	Foam stability varies according to foam composition, volume, and injection technique.
26671207	1	58	theme	effective	120:128	arg1	strategy					140:147	an effective treatment strategy	117:147	an effective treatment strategy for varicose veins and venous malformations	117:191	BACKGROUND Foam sclerotherapy is an effective treatment strategy for varicose veins and venous malformations.
26671207	1	58	theme	effective	120:128	arg1	sclerotherapy					100:112	BACKGROUND Foam sclerotherapy	84:112	BACKGROUND Foam sclerotherapy	84:112	BACKGROUND Foam sclerotherapy is an effective treatment strategy for varicose veins and venous malformations.
26671207	6	59	theme	original	701:708	arg1	volume					710:715	its original volume	697:715	its original volume	697:715	The half-life, or the time for a volume of foam to be reduced to half of its original volume, was used to evaluate foam stability.
26671207	8	60	theme	HA	941:942	arg1	mL					932:933	0.05 mL	927:933	0.05 mL of 1% HA	927:942	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	9	61	theme	amounts	1093:1099	arg1	addition					1075:1082	the addition	1071:1082	the addition of small amounts of HA	1071:1105	CONCLUSION The stability of POL foam was highly increased by the addition of small amounts of HA.
26671207	8	62	theme	%	939:939	arg1	HA					941:942	1% HA	938:942	1% HA	938:942	RESULTS The half-life was 142.8 (±4.32) seconds for 1% POL without the addition of HA, 310.6 (±7.53) seconds with the addition of 0.05 mL of 1% HA, and 390.4 (±13.06) seconds with the addition of 0.1 mL of 1% HA.
26671207	1	63	theme	treatment	130:138	arg1	strategy					140:147	an effective treatment strategy	117:147	an effective treatment strategy for varicose veins and venous malformations	117:191	BACKGROUND Foam sclerotherapy is an effective treatment strategy for varicose veins and venous malformations.
26671207	1	63	theme	treatment	130:138	arg1	sclerotherapy					100:112	BACKGROUND Foam sclerotherapy	84:112	BACKGROUND Foam sclerotherapy	84:112	BACKGROUND Foam sclerotherapy is an effective treatment strategy for varicose veins and venous malformations.
26671207	9	64	theme	foam	1042:1045	arg1	stability					1025:1033	The stability	1021:1033	The stability of POL foam	1021:1045	CONCLUSION The stability of POL foam was highly increased by the addition of small amounts of HA.
26671207	3	65	theme	POL	332:334	arg1	foam					337:340	polidocanol (POL) foam	319:340	polidocanol (POL) foam	319:340	OBJECTIVE To evaluate the stability of polidocanol (POL) foam with the addition of hyaluronic acid (HA).
26671207	2	66	theme	Foam	194:197	arg1	stability					199:207	Foam stability	194:207	Foam stability	194:207	Foam stability varies according to foam composition, volume, and injection technique.
26671207	4	67	theme	1	556:556	arg1	%					557:557	%	557:557	%	557:557	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	9	68	theme	POL	1038:1040	arg1	foam					1042:1045	POL foam	1038:1045	POL foam	1038:1045	CONCLUSION The stability of POL foam was highly increased by the addition of small amounts of HA.
26671207	6	69	theme	foam	739:742	arg1	stability					744:752	foam stability	739:752	foam stability	739:752	The half-life, or the time for a volume of foam to be reduced to half of its original volume, was used to evaluate foam stability.
26671207	4	70	theme	%	480:480	arg1	POL					482:484	1% POL	479:484	1% POL	479:484	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	9	71	dep	CONCLUSION	1010:1019	arg1	increased					1058:1066	increased	1058:1066	was highly increased by the addition of small amounts of HA	1047:1105	CONCLUSION The stability of POL foam was highly increased by the addition of small amounts of HA.
26671207	4	72	dep	MATERIALS	385:393	arg1	mL					435:436	0 mL	433:436	0 mL of 1% HA	433:445	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	4	72	dep	MATERIALS	385:393	arg1	A					413:413	Group A	407:413	Group A	407:413	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	4	72	dep	MATERIALS	385:393	arg1	mL					418:419	2 mL	416:419	2 mL of 1% POL	416:429	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26671207	4	73	theme	1	479:479	arg1	%					480:480	%	480:480	%	480:480	MATERIALS AND METHODS Group A: 2 mL of 1% POL + 0 mL of 1% HA + 8 mL of air; Group B: 2 mL of 1% POL + 0.05 mL of 1% HA + 8 mL of air; Group C: 2 mL of 1% POL + 0.1 mL of 1% HA + 8 mL of air.
26700236	9	0	dep	9	1272:1272	arg1	to					1269:1270	to	1269:1270	to	1269:1270	The LbL reservoirs were highly effective for the long-term storage (up to 9 months) of aFGF and bFGF.
26700236	5	1	theme	release	623:629	arg1	assays					631:636	Stability and release assays	609:636	Stability and release assays	609:636	Stability and release assays were performed in a phosphate buffer at physiological conditions.
26700236	9	2	theme	bFGF	1294:1297	arg1	months					1274:1279	up to 9 months	1266:1279	up to 9 months	1266:1279	The LbL reservoirs were highly effective for the long-term storage (up to 9 months) of aFGF and bFGF.
26700236	9	2	theme	bFGF	1294:1297	arg1	storage					1257:1263	the long-term storage	1243:1263	the long-term storage (up to 9 months) of aFGF and bFGF	1243:1297	The LbL reservoirs were highly effective for the long-term storage (up to 9 months) of aFGF and bFGF.
26700236	1	3	theme	basic	209:213	arg1	factors					233:239	fibroblast growth factors	215:239	acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively)	198:271	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi) were prepared for use as reservoirs for acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively).
26700236	0	4	theme	3T3-cells	79:87	arg1	response					67:74	the response	63:74	the response of 3T3-cells	63:87	Layer-by-Layer assembled growth factor reservoirs for steering the response of 3T3-cells.
26700236	7	5	theme	fibroblasts	1071:1081	arg1	proliferation					1042:1054	the proliferation	1038:1054	the proliferation of the NIH-3T3 fibroblasts	1038:1081	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	2	6	from	effects	278:284	arg1	proliferation					361:373	proliferation	361:373	proliferation	361:373	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	2	6	from	effects	278:284	arg1	viability					347:355	viability	347:355	viability	347:355	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	2	7	theme	architecture	293:304	arg1	effects					278:284	The effects	274:284	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells	274:401	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	1	8	theme	fibroblast	215:224	arg1	factors					233:239	fibroblast growth factors	215:239	acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively)	198:271	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi) were prepared for use as reservoirs for acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively).
26700236	10	9	theme	LbL	1340:1342	arg1	reservoirs					1344:1353	LbL reservoirs	1340:1353	LbL reservoirs	1340:1353	This work demonstrates the potential of LbL reservoirs for use as biomaterial coatings.
26700236	7	10	theme	low	883:885	arg1	LSCM					909:912	LSCM	909:912	LSCM	909:912	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	10	theme	low	883:885	arg1	medium					901:906	low serum culture medium	883:906	low serum culture medium (LSCM)	883:913	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	1	11	theme	growth	226:231	arg1	factors					233:239	fibroblast growth factors	215:239	acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively)	198:271	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi) were prepared for use as reservoirs for acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively).
26700236	4	12	theme	dissipation-enhanced	551:570	arg1	microbalance					587:598	a dissipation-enhanced quartz crystal microbalance	549:598	a dissipation-enhanced quartz crystal microbalance (QCM-D)	549:606	The aFGF and bFGF loadings were determined using a dissipation-enhanced quartz crystal microbalance (QCM-D).
26700236	4	12	theme	dissipation-enhanced	551:570	arg1	QCM-D					601:605	QCM-D	601:605	QCM-D	601:605	The aFGF and bFGF loadings were determined using a dissipation-enhanced quartz crystal microbalance (QCM-D).
26700236	8	13	theme	n	1126:1126	arg1	assemblies					1128:1137	(Hep/Chi)n assemblies	1117:1137	(Hep/Chi)n assemblies prepared in the absence of FGF under identical conditions	1117:1195	The cells did not proliferate on (Hep/Chi)n assemblies prepared in the absence of FGF under identical conditions.
26700236	8	14	theme	FGF	1166:1168	arg1	absence					1155:1161	the absence	1151:1161	the absence of FGF	1151:1168	The cells did not proliferate on (Hep/Chi)n assemblies prepared in the absence of FGF under identical conditions.
26700236	9	15	theme	long-term	1247:1255	arg1	months					1274:1279	up to 9 months	1266:1279	up to 9 months	1266:1279	The LbL reservoirs were highly effective for the long-term storage (up to 9 months) of aFGF and bFGF.
26700236	9	15	theme	long-term	1247:1255	arg1	storage					1257:1263	the long-term storage	1243:1263	the long-term storage (up to 9 months) of aFGF and bFGF	1243:1297	The LbL reservoirs were highly effective for the long-term storage (up to 9 months) of aFGF and bFGF.
26700236	10	16	theme	biomaterial	1366:1376	arg1	coatings					1378:1385	biomaterial coatings	1366:1385	biomaterial coatings	1366:1385	This work demonstrates the potential of LbL reservoirs for use as biomaterial coatings.
26700236	7	17	theme	culture	893:899	arg1	LSCM					909:912	LSCM	909:912	LSCM	909:912	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	17	theme	culture	893:899	arg1	medium					901:906	low serum culture medium	883:906	low serum culture medium (LSCM)	883:913	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	8	18	theme	Hep/Chi	1118:1124	arg1	assemblies					1128:1137	(Hep/Chi)n assemblies	1117:1137	(Hep/Chi)n assemblies prepared in the absence of FGF under identical conditions	1117:1195	The cells did not proliferate on (Hep/Chi)n assemblies prepared in the absence of FGF under identical conditions.
26700236	5	19	theme	Stability	609:617	arg1	assays					631:636	Stability and release assays	609:636	Stability and release assays	609:636	Stability and release assays were performed in a phosphate buffer at physiological conditions.
26700236	1	20	theme	Layer-by-Layer	90:103	arg1	assemblies					111:120	Layer-by-Layer (LbL) assemblies	90:120	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi)	90:156	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi) were prepared for use as reservoirs for acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively).
26700236	2	21	theme	reservoirs	329:338	arg1	composition					310:320	composition	310:320	composition	310:320	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	2	21	theme	reservoirs	329:338	arg1	architecture					293:304	architecture	293:304	architecture	293:304	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	2	22	theme	cells	397:401	arg1	proliferation					361:373	proliferation	361:373	proliferation	361:373	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	2	22	theme	cells	397:401	arg1	viability					347:355	viability	347:355	viability	347:355	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	6	23	theme	bFGF	757:760	arg1	amount					738:743	the amount	734:743	the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs	734:837	The results demonstrated that the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs could be controlled.
26700236	6	23	theme	bFGF	757:760	arg1	bFGF					757:760	bFGF	757:760	bFGF	757:760	The results demonstrated that the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs could be controlled.
26700236	6	23	theme	bFGF	757:760	arg1	aFGF					748:751	aFGF	748:751	aFGF	748:751	The results demonstrated that the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs could be controlled.
26700236	10	24	theme	reservoirs	1344:1353	arg1	potential					1327:1335	the potential	1323:1335	the potential of LbL reservoirs for use as biomaterial coatings	1323:1385	This work demonstrates the potential of LbL reservoirs for use as biomaterial coatings.
26700236	0	25	theme	factor	32:37	arg1	reservoirs					39:48	growth factor reservoirs	25:48	growth factor reservoirs for steering the response of 3T3-cells	25:87	Layer-by-Layer assembled growth factor reservoirs for steering the response of 3T3-cells.
26700236	4	26	theme	bFGF	513:516	arg1	loadings					518:525	The aFGF and bFGF loadings	500:525	The aFGF and bFGF loadings	500:525	The aFGF and bFGF loadings were determined using a dissipation-enhanced quartz crystal microbalance (QCM-D).
26700236	7	27	theme	n	1001:1001	arg1	multilayers					1003:1013	the (Hep/Chi)n multilayers	988:1013	the (Hep/Chi)n multilayers	988:1013	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	28	theme	serum	887:891	arg1	LSCM					909:912	LSCM	909:912	LSCM	909:912	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	28	theme	serum	887:891	arg1	medium					901:906	low serum culture medium	883:906	low serum culture medium (LSCM)	883:913	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	1	29	theme	LbL	106:108	arg1	assemblies					111:120	Layer-by-Layer (LbL) assemblies	90:120	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi)	90:156	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi) were prepared for use as reservoirs for acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively).
26700236	6	30	theme	LbL	792:794	arg1	reservoirs					796:805	LbL reservoirs	792:805	LbL reservoirs composed of 3 and 6 layer pairs	792:837	The results demonstrated that the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs could be controlled.
26700236	2	31	theme	NIH-3T3	378:384	arg1	cells					397:401	NIH-3T3 fibroblast cells	378:401	NIH-3T3 fibroblast cells	378:401	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	5	32	theme	physiological	678:690	arg1	conditions					692:701	physiological conditions	678:701	physiological conditions	678:701	Stability and release assays were performed in a phosphate buffer at physiological conditions.
26700236	1	33	theme	chitosan	143:150	arg1	assemblies					111:120	Layer-by-Layer (LbL) assemblies	90:120	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi)	90:156	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi) were prepared for use as reservoirs for acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively).
26700236	7	34	theme	Hep/Chi	993:999	arg1	multilayers					1003:1013	the (Hep/Chi)n multilayers	988:1013	the (Hep/Chi)n multilayers	988:1013	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	6	35	theme	layer	827:831	arg1	pairs					833:837	3 and 6 layer pairs	819:837	3 and 6 layer pairs	819:837	The results demonstrated that the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs could be controlled.
26700236	2	36	theme	starvation	422:431	arg1	conditions					433:442	starvation conditions	422:442	starvation conditions	422:442	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	7	37	theme	NIH-3T3	1063:1069	arg1	fibroblasts					1071:1081	the NIH-3T3 fibroblasts	1059:1081	the NIH-3T3 fibroblasts	1059:1081	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	2	38	dep	viability	347:355	arg1	the					343:345	the	343:345	the	343:345	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	6	39	theme	aFGF	748:751	arg1	amount					738:743	the amount	734:743	the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs	734:837	The results demonstrated that the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs could be controlled.
26700236	6	39	theme	aFGF	748:751	arg1	bFGF					757:760	bFGF	757:760	bFGF	757:760	The results demonstrated that the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs could be controlled.
26700236	6	39	theme	aFGF	748:751	arg1	aFGF					748:751	aFGF	748:751	aFGF	748:751	The results demonstrated that the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs could be controlled.
26700236	9	40	theme	aFGF	1285:1288	arg1	months					1274:1279	up to 9 months	1266:1279	up to 9 months	1266:1279	The LbL reservoirs were highly effective for the long-term storage (up to 9 months) of aFGF and bFGF.
26700236	9	40	theme	aFGF	1285:1288	arg1	storage					1257:1263	the long-term storage	1243:1263	the long-term storage (up to 9 months) of aFGF and bFGF	1243:1297	The LbL reservoirs were highly effective for the long-term storage (up to 9 months) of aFGF and bFGF.
26700236	7	41	theme	bFGF	978:981	arg1	bFGF					978:981	bFGF	978:981	bFGF	978:981	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	41	theme	bFGF	978:981	arg1	aFGF					969:972	aFGF	969:972	aFGF	969:972	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	41	theme	bFGF	978:981	arg1	amounts					958:964	very small amounts	947:964	very small amounts of aFGF and bFGF	947:981	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	3	42	theme	reservoir	449:457	arg1	stability					459:467	The reservoir stability	445:467	The reservoir stability	445:467	The reservoir stability was monitored by ellipsometry.
26700236	2	43	theme	fibroblast	386:395	arg1	cells					397:401	NIH-3T3 fibroblast cells	378:401	NIH-3T3 fibroblast cells	378:401	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	4	44	theme	aFGF	504:507	arg1	loadings					518:525	The aFGF and bFGF loadings	500:525	The aFGF and bFGF loadings	500:525	The aFGF and bFGF loadings were determined using a dissipation-enhanced quartz crystal microbalance (QCM-D).
26700236	5	45	theme	phosphate	658:666	arg1	buffer					668:673	a phosphate buffer	656:673	a phosphate buffer	656:673	Stability and release assays were performed in a phosphate buffer at physiological conditions.
26700236	1	46	dep	factors	233:239	arg1	bFGFs					252:256	bFGFs	252:256	bFGFs	252:256	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi) were prepared for use as reservoirs for acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively).
26700236	1	46	dep	factors	233:239	arg1	aFGFs					242:246	aFGFs	242:246	aFGFs	242:246	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi) were prepared for use as reservoirs for acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively).
26700236	8	47	theme	identical	1176:1184	arg1	conditions					1186:1195	identical conditions	1176:1195	identical conditions	1176:1195	The cells did not proliferate on (Hep/Chi)n assemblies prepared in the absence of FGF under identical conditions.
26700236	1	48	theme	heparin	125:131	arg1	assemblies					111:120	Layer-by-Layer (LbL) assemblies	90:120	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi)	90:156	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi) were prepared for use as reservoirs for acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively).
26700236	7	49	from	assays	873:878	arg1	LSCM					909:912	LSCM	909:912	LSCM	909:912	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	49	from	assays	873:878	arg1	medium					901:906	low serum culture medium	883:906	low serum culture medium (LSCM)	883:913	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	50	theme	aFGF	969:972	arg1	bFGF					978:981	bFGF	978:981	bFGF	978:981	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	50	theme	aFGF	969:972	arg1	aFGF					969:972	aFGF	969:972	aFGF	969:972	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	50	theme	aFGF	969:972	arg1	amounts					958:964	very small amounts	947:964	very small amounts of aFGF and bFGF	947:981	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	51	theme	Cell	860:863	arg1	assays					873:878	Cell culture assays	860:878	Cell culture assays in low serum culture medium (LSCM)	860:913	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	4	52	theme	crystal	579:585	arg1	microbalance					587:598	a dissipation-enhanced quartz crystal microbalance	549:598	a dissipation-enhanced quartz crystal microbalance (QCM-D)	549:606	The aFGF and bFGF loadings were determined using a dissipation-enhanced quartz crystal microbalance (QCM-D).
26700236	4	52	theme	crystal	579:585	arg1	QCM-D					601:605	QCM-D	601:605	QCM-D	601:605	The aFGF and bFGF loadings were determined using a dissipation-enhanced quartz crystal microbalance (QCM-D).
26700236	9	53	theme	LbL	1202:1204	arg1	reservoirs					1206:1215	The LbL reservoirs	1198:1215	The LbL reservoirs	1198:1215	The LbL reservoirs were highly effective for the long-term storage (up to 9 months) of aFGF and bFGF.
26700236	9	53	theme	LbL	1202:1204	arg1	effective					1229:1237	effective	1229:1237	effective	1229:1237	The LbL reservoirs were highly effective for the long-term storage (up to 9 months) of aFGF and bFGF.
26700236	7	54	theme	small	952:956	arg1	bFGF					978:981	bFGF	978:981	bFGF	978:981	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	54	theme	small	952:956	arg1	aFGF					969:972	aFGF	969:972	aFGF	969:972	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	54	theme	small	952:956	arg1	amounts					958:964	very small amounts	947:964	very small amounts of aFGF and bFGF	947:981	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	7	55	theme	culture	865:871	arg1	assays					873:878	Cell culture assays	860:878	Cell culture assays in low serum culture medium (LSCM)	860:913	Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
26700236	1	56	theme	acidic	198:203	arg1	factors					233:239	fibroblast growth factors	215:239	acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively)	198:271	Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi) were prepared for use as reservoirs for acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively).
26700236	4	57	theme	quartz	572:577	arg1	microbalance					587:598	a dissipation-enhanced quartz crystal microbalance	549:598	a dissipation-enhanced quartz crystal microbalance (QCM-D)	549:606	The aFGF and bFGF loadings were determined using a dissipation-enhanced quartz crystal microbalance (QCM-D).
26700236	4	57	theme	quartz	572:577	arg1	QCM-D					601:605	QCM-D	601:605	QCM-D	601:605	The aFGF and bFGF loadings were determined using a dissipation-enhanced quartz crystal microbalance (QCM-D).
26700236	0	58	theme	growth	25:30	arg1	reservoirs					39:48	growth factor reservoirs	25:48	growth factor reservoirs for steering the response of 3T3-cells	25:87	Layer-by-Layer assembled growth factor reservoirs for steering the response of 3T3-cells.
26700236	2	59	theme	composition	310:320	arg1	effects					278:284	The effects	274:284	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells	274:401	The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions.
26700236	6	60	dep	amount	738:743	arg1	loaded					762:767	loaded	762:767	loaded into	762:772	The results demonstrated that the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs could be controlled.
26700236	6	60	dep	amount	738:743	arg1	released					778:785	released	778:785	released from LbL reservoirs composed of 3 and 6 layer pairs	778:837	The results demonstrated that the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs could be controlled.
29352926	4	0	theme	high	444:447	arg1	aldehyde					435:442	aldehyde high	435:447	aldehyde high	435:447	Importantly, high oxidation degree makes the content of aldehyde high, which make the cell biocompatibility poor.
29352926	7	1	theme	%	809:809	arg1	Arg					810:812	MOBC/68.68%Arg	799:812	MOBC/68.68%Arg	799:812	The roughness and surface energy of MOBC/68.68%Arg are 1.5 and 1.16 times than that of BC respectively.
29352926	8	2	theme	cell	914:917	arg1	migration					919:927	the cell migration	910:927	the cell migration	910:927	We applied a microfluidic chip to evaluate the cell migration.
29352926	10	3	theme	wound	1100:1104	arg1	MOBC/Arg					1076:1083	the MOBC/Arg	1072:1083	the MOBC/Arg	1072:1083	It prospects the MOBC/Arg can be used as wound dressing.
29352926	10	3	theme	wound	1100:1104	arg1	dressing					1106:1113	wound dressing	1100:1113	wound dressing	1100:1113	It prospects the MOBC/Arg can be used as wound dressing.
29352926	0	4	theme	fibroblast/endothelial	98:119	arg1	cell					121:124	fibroblast/endothelial cell	98:124	fibroblast/endothelial cell	98:124	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	8	5	theme	microfluidic	880:891	arg1	chip					893:896	a microfluidic chip	878:896	a microfluidic chip to evaluate the cell migration	878:927	We applied a microfluidic chip to evaluate the cell migration.
29352926	9	6	theme	fibroblasts	1024:1034	arg1	Collagen-I					1010:1019	Collagen-I	1010:1019	Collagen-I of fibroblasts and endothelial cells	1010:1056	Comparing with BC, MOBC/Arg promoted proliferation, migration and expression of Collagen-I of fibroblasts and endothelial cells.
29352926	5	7	theme	microporous	546:556	arg1	MOBC					571:574	MOBC	571:574	MOBC	571:574	Herein, we fabricated a novel bio-composite based on microporous oxidized BC (MOBC) and in-situ grafted with Arg.
29352926	5	7	theme	microporous	546:556	arg1	BC					567:568	microporous oxidized BC	546:568	microporous oxidized BC (MOBC)	546:575	Herein, we fabricated a novel bio-composite based on microporous oxidized BC (MOBC) and in-situ grafted with Arg.
29352926	6	8	theme	contact	639:645	arg1	area					647:650	the contact area	635:650	the contact area between BC and oxidizing agent	635:681	The micropores can increase the contact area between BC and oxidizing agent and the reaction between MOBC and Arg, which will enhance the biocompatibility.
29352926	0	9	from	composite	58:66	arg1	behavior					86:93	behavior	86:93	behavior	86:93	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	5	10	theme	oxidized	558:565	arg1	MOBC					571:574	MOBC	571:574	MOBC	571:574	Herein, we fabricated a novel bio-composite based on microporous oxidized BC (MOBC) and in-situ grafted with Arg.
29352926	5	10	theme	oxidized	558:565	arg1	BC					567:568	microporous oxidized BC	546:568	microporous oxidized BC (MOBC)	546:575	Herein, we fabricated a novel bio-composite based on microporous oxidized BC (MOBC) and in-situ grafted with Arg.
29352926	7	11	theme	surface	781:787	arg1	roughness					767:775	roughness	767:775	roughness	767:775	The roughness and surface energy of MOBC/68.68%Arg are 1.5 and 1.16 times than that of BC respectively.
29352926	7	11	theme	surface	781:787	arg1	energy					789:794	surface energy	781:794	surface energy	781:794	The roughness and surface energy of MOBC/68.68%Arg are 1.5 and 1.16 times than that of BC respectively.
29352926	7	11	theme	surface	781:787	arg1	times					831:835	1.5 and 1.16 times	818:835	1.5 and 1.16 times	818:835	The roughness and surface energy of MOBC/68.68%Arg are 1.5 and 1.16 times than that of BC respectively.
29352926	0	12	theme	cell	121:124	arg1	cell					121:124	fibroblast/endothelial cell	98:124	fibroblast/endothelial cell	98:124	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	0	12	theme	cell	121:124	arg1	composite					58:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	0	12	theme	cell	121:124	arg1	effect					76:81	its effect	72:81	its effect on behavior of fibroblast/endothelial cell	72:124	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	4	13	theme	aldehyde	435:442	arg1	content					424:430	the content	420:430	the content	420:430	Importantly, high oxidation degree makes the content of aldehyde high, which make the cell biocompatibility poor.
29352926	2	14	used	used	287:290	arg2	BC					249:250	the oxidized BC	236:250	the oxidized BC	236:250	Although there are many methods to modify BC, such as the oxidized BC, which is biodegradable and can be used as wound dressing.
29352926	2	14	used	used	287:290	arg2	dressing					301:308	wound dressing	295:308	wound dressing	295:308	Although there are many methods to modify BC, such as the oxidized BC, which is biodegradable and can be used as wound dressing.
29352926	2	14	used	used	287:290	arg2	biodegradable					262:274	biodegradable	262:274	biodegradable	262:274	Although there are many methods to modify BC, such as the oxidized BC, which is biodegradable and can be used as wound dressing.
29352926	0	15	theme	novel	2:6	arg1	cell					121:124	fibroblast/endothelial cell	98:124	fibroblast/endothelial cell	98:124	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	0	15	theme	novel	2:6	arg1	composite					58:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	5	16	theme	novel	517:521	arg1	bio-composite					523:535	a novel bio-composite	515:535	a novel bio-composite based on microporous oxidized BC (MOBC) and in-situ grafted with Arg	515:604	Herein, we fabricated a novel bio-composite based on microporous oxidized BC (MOBC) and in-situ grafted with Arg.
29352926	0	17	theme	oxidized	20:27	arg1	cell					121:124	fibroblast/endothelial cell	98:124	fibroblast/endothelial cell	98:124	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	0	17	theme	oxidized	20:27	arg1	composite					58:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	0	18	theme	microporous	8:18	arg1	cell					121:124	fibroblast/endothelial cell	98:124	fibroblast/endothelial cell	98:124	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	0	18	theme	microporous	8:18	arg1	composite					58:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	4	19	theme	oxidation	397:405	arg1	degree					407:412	high oxidation degree	392:412	high oxidation degree	392:412	Importantly, high oxidation degree makes the content of aldehyde high, which make the cell biocompatibility poor.
29352926	0	20	theme	cellulose/arginine	39:56	arg1	cell					121:124	fibroblast/endothelial cell	98:124	fibroblast/endothelial cell	98:124	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	0	20	theme	cellulose/arginine	39:56	arg1	composite					58:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	2	21	theme	wound	295:299	arg1	BC					249:250	the oxidized BC	236:250	the oxidized BC	236:250	Although there are many methods to modify BC, such as the oxidized BC, which is biodegradable and can be used as wound dressing.
29352926	2	21	theme	wound	295:299	arg1	dressing					301:308	wound dressing	295:308	wound dressing	295:308	Although there are many methods to modify BC, such as the oxidized BC, which is biodegradable and can be used as wound dressing.
29352926	4	22	theme	high	392:395	arg1	degree					407:412	high oxidation degree	392:412	high oxidation degree	392:412	Importantly, high oxidation degree makes the content of aldehyde high, which make the cell biocompatibility poor.
29352926	9	23	theme	Collagen-I	1010:1019	arg1	expression					996:1005	expression	996:1005	expression	996:1005	Comparing with BC, MOBC/Arg promoted proliferation, migration and expression of Collagen-I of fibroblasts and endothelial cells.
29352926	9	23	theme	Collagen-I	1010:1019	arg1	migration					982:990	migration	982:990	migration	982:990	Comparing with BC, MOBC/Arg promoted proliferation, migration and expression of Collagen-I of fibroblasts and endothelial cells.
29352926	9	23	theme	Collagen-I	1010:1019	arg1	proliferation					967:979	proliferation	967:979	proliferation	967:979	Comparing with BC, MOBC/Arg promoted proliferation, migration and expression of Collagen-I of fibroblasts and endothelial cells.
29352926	0	24	theme	bacterial	29:37	arg1	cell					121:124	fibroblast/endothelial cell	98:124	fibroblast/endothelial cell	98:124	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	0	24	theme	bacterial	29:37	arg1	composite					58:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite	0:66	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	4	25	theme	cell	465:468	arg1	biocompatibility					470:485	the cell biocompatibility poor	461:490	the cell biocompatibility poor	461:490	Importantly, high oxidation degree makes the content of aldehyde high, which make the cell biocompatibility poor.
29352926	0	26	from	effect	76:81	arg1	behavior					86:93	behavior	86:93	behavior	86:93	A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell.
29352926	7	27	dep	roughness	767:775	arg1	The					763:765	The	763:765	The	763:765	The roughness and surface energy of MOBC/68.68%Arg are 1.5 and 1.16 times than that of BC respectively.
29352926	4	28	theme	poor	487:490	arg1	biocompatibility					470:485	the cell biocompatibility poor	461:490	the cell biocompatibility poor	461:490	Importantly, high oxidation degree makes the content of aldehyde high, which make the cell biocompatibility poor.
29352926	1	29	theme	bacterial	131:139	arg1	cellulose					141:149	The bacterial cellulose	127:149	The bacterial cellulose (BC)	127:154	The bacterial cellulose (BC) has been reported widely.
29352926	1	29	theme	bacterial	131:139	arg1	BC					152:153	BC	152:153	BC	152:153	The bacterial cellulose (BC) has been reported widely.
29352926	2	30	theme	many	201:204	arg1	methods					206:212	many methods	201:212	many methods	201:212	Although there are many methods to modify BC, such as the oxidized BC, which is biodegradable and can be used as wound dressing.
29352926	2	31	theme	oxidized	240:247	arg1	BC					249:250	the oxidized BC	236:250	the oxidized BC	236:250	Although there are many methods to modify BC, such as the oxidized BC, which is biodegradable and can be used as wound dressing.
29352926	2	31	theme	oxidized	240:247	arg1	dressing					301:308	wound dressing	295:308	wound dressing	295:308	Although there are many methods to modify BC, such as the oxidized BC, which is biodegradable and can be used as wound dressing.
29352926	2	31	theme	oxidized	240:247	arg1	biodegradable					262:274	biodegradable	262:274	biodegradable	262:274	Although there are many methods to modify BC, such as the oxidized BC, which is biodegradable and can be used as wound dressing.
29352926	6	32	theme	oxidizing	667:675	arg1	agent					677:681	oxidizing agent	667:681	oxidizing agent	667:681	The micropores can increase the contact area between BC and oxidizing agent and the reaction between MOBC and Arg, which will enhance the biocompatibility.
29352926	9	33	theme	endothelial	1040:1050	arg1	cells					1052:1056	endothelial cells	1040:1056	endothelial cells	1040:1056	Comparing with BC, MOBC/Arg promoted proliferation, migration and expression of Collagen-I of fibroblasts and endothelial cells.
29352926	9	34	theme	cells	1052:1056	arg1	Collagen-I					1010:1019	Collagen-I	1010:1019	Collagen-I of fibroblasts and endothelial cells	1010:1056	Comparing with BC, MOBC/Arg promoted proliferation, migration and expression of Collagen-I of fibroblasts and endothelial cells.
29352926	7	35	theme	Arg	810:812	arg1	roughness					767:775	roughness	767:775	roughness	767:775	The roughness and surface energy of MOBC/68.68%Arg are 1.5 and 1.16 times than that of BC respectively.
29352926	7	35	theme	Arg	810:812	arg1	energy					789:794	surface energy	781:794	surface energy	781:794	The roughness and surface energy of MOBC/68.68%Arg are 1.5 and 1.16 times than that of BC respectively.
29352926	7	35	theme	Arg	810:812	arg1	times					831:835	1.5 and 1.16 times	818:835	1.5 and 1.16 times	818:835	The roughness and surface energy of MOBC/68.68%Arg are 1.5 and 1.16 times than that of BC respectively.
29352926	10	36	used	used	1092:1095	arg2	dressing					1106:1113	wound dressing	1100:1113	wound dressing	1100:1113	It prospects the MOBC/Arg can be used as wound dressing.
29352926	10	36	used	used	1092:1095	arg2	MOBC/Arg					1076:1083	the MOBC/Arg	1072:1083	the MOBC/Arg	1072:1083	It prospects the MOBC/Arg can be used as wound dressing.
29352926	3	37	theme	BC	341:342	arg1	nanostructure					324:336	the nanostructure	320:336	the nanostructure of BC	320:342	However, the nanostructure of BC makes it difficult to be oxidized.
29352926	7	38	theme	MOBC/68.68	799:808	arg1	%					809:809	%	809:809	%	809:809	The roughness and surface energy of MOBC/68.68%Arg are 1.5 and 1.16 times than that of BC respectively.
25412752	9	0	theme	=	1395:1395	arg1	efficiency					1370:1379	efficiency	1370:1379	efficiency for growth (P = 0.025)	1370:1402	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	9	0	theme	=	1395:1395	arg1	P					1393:1393	P = 0.025	1393:1401	P = 0.025	1393:1401	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	7	1	theme	n-3	1029:1031	arg1	acids					1055:1059	n-6 and n-3 polyunsaturated fatty acids	1021:1059	acids	1055:1059	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	7	2	theme	Meat	931:934	arg1	traits					956:961	Meat nutritional quality traits	931:961	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids)	931:1060	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	1	3	theme	rich	265:268	arg1	diets					259:263	high-concentrate diets	242:263	high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products	242:352	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	12	4	theme	lower	1682:1686	arg1	gain					1691:1694	lower BW gain	1682:1694	lower BW gain (P < 0.001)	1682:1706	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	12	4	theme	lower	1682:1686	arg1	<					1699:1699	P < 0.001	1697:1705	P < 0.001	1697:1705	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	3	5	theme	rich	541:544	arg1	mixtures					532:539	concentrate mixtures	520:539	concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw	520:665	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	3	6	theme	diet	495:498	arg1	treatments					500:509	The diet treatments	491:509	The diet treatments	491:509	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	14	7	contain	have	1939:1942	arg1	bulls					1869:1873	SLR bulls	1865:1873	SLR bulls	1865:1873	Compared to bulls fed the FR diet, SLR bulls had lower than planned NEg intake and lower BW gain but did not have differences in body composition.
25412752	14	7	contain	have	1939:1942	arg2	differences					1944:1954	differences	1944:1954	differences in body composition	1944:1974	Compared to bulls fed the FR diet, SLR bulls had lower than planned NEg intake and lower BW gain but did not have differences in body composition.
25412752	13	8	theme	lower	1774:1778	arg1	weight					1788:1793	lower carcass weight	1774:1793	lower carcass weight	1774:1793	They had lower carcass weight but a greater muscle-to-fat ratio.
25412752	14	9	theme	body	1959:1962	arg1	composition					1964:1974	body composition	1959:1974	body composition	1959:1974	Compared to bulls fed the FR diet, SLR bulls had lower than planned NEg intake and lower BW gain but did not have differences in body composition.
25412752	12	10	theme	less	1662:1665	arg1	energy					1667:1672	15% less energy	1658:1672	15% less energy	1658:1672	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	14	11	from	differences	1944:1954	arg1	composition					1964:1974	body composition	1959:1974	body composition	1959:1974	Compared to bulls fed the FR diet, SLR bulls had lower than planned NEg intake and lower BW gain but did not have differences in body composition.
25412752	10	12	theme	greater	1497:1503	arg1	proportion					1505:1514	a greater proportion	1495:1514	a greater proportion of fat	1495:1521	They also had heavier carcasses with a greater proportion of fat.
25412752	3	13	dep	fiber	556:560	arg1	=					569:569	=	569:569	=	569:569	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	4	14	theme	FR	763:764	arg1	diet					766:769	the FR diet	759:769	the FR diet in the SLR diet	759:785	The concentrate mix was offered ad libitum in SL and FR diets but was kept isoenergetic to the FR diet in the SLR diet.
25412752	12	15	theme	P	1753:1753	arg1	0.010					1757:1761	P = 0.010	1753:1761	P = 0.010	1753:1761	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	12	15	theme	P	1753:1753	arg1	growth					1745:1750	growth	1745:1750	growth (P = 0.010)	1745:1762	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	1	16	theme	fattening	195:203	arg1	performance					205:215	fattening performance	195:215	fattening performance	195:215	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	1	17	from	rich	265:268	arg1	lipids					291:296	lipids	291:296	lipids	291:296	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	1	17	from	rich	265:268	arg1	starch					280:285	starch	280:285	starch	280:285	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	1	17	from	rich	265:268	arg1	by-products					342:352	fibrous by-products	334:352	fibrous by-products	334:352	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	9	18	theme	diet	1446:1449	arg1	bulls					1451:1455	FR diet bulls	1443:1455	FR diet bulls	1443:1455	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	15	19	theme	fatty	2039:2043	arg1	acid					2045:2048	a greater omega-3 fatty acid	2021:2048	a greater omega-3 fatty acid content	2021:2056	Compared to the FR diet, the SL diet led to a greater omega-3 fatty acid content because of a greater supply of dietary linoleic acid, especially in lean muscle.
25412752	15	20	theme	greater	2023:2029	arg1	acid					2045:2048	a greater omega-3 fatty acid	2021:2048	a greater omega-3 fatty acid content	2021:2056	Compared to the FR diet, the SL diet led to a greater omega-3 fatty acid content because of a greater supply of dietary linoleic acid, especially in lean muscle.
25412752	9	21	theme	diet	1299:1302	arg1	bulls					1304:1308	The SL diet bulls	1292:1308	The SL diet bulls	1292:1308	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	7	22	theme	semitendinosus	1127:1140	arg1	muscles					1142:1148	semitendinosus muscles	1127:1148	semitendinosus muscles	1127:1148	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	3	23	theme	barley	654:659	arg1	straw					661:665	starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw	578:665	starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw	578:665	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	14	24	theme	lower	1913:1917	arg1	gain					1922:1925	lower BW gain	1913:1925	lower BW gain	1913:1925	Compared to bulls fed the FR diet, SLR bulls had lower than planned NEg intake and lower BW gain but did not have differences in body composition.
25412752	0	25	theme	bulls	71:75	arg1	composition					56:66	muscle composition	49:66	muscle composition	49:66	Fattening performance, metabolic indicators, and muscle composition of bulls fed fiber-rich versus starch-plus-lipid-rich concentrate diets.
25412752	0	25	theme	bulls	71:75	arg1	performance					10:20	Fattening performance	0:20	Fattening performance	0:20	Fattening performance, metabolic indicators, and muscle composition of bulls fed fiber-rich versus starch-plus-lipid-rich concentrate diets.
25412752	0	25	theme	bulls	71:75	arg1	indicators					33:42	metabolic indicators	23:42	metabolic indicators	23:42	Fattening performance, metabolic indicators, and muscle composition of bulls fed fiber-rich versus starch-plus-lipid-rich concentrate diets.
25412752	1	26	theme	fibrous	334:340	arg1	by-products					342:352	fibrous by-products	334:352	fibrous by-products	334:352	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	12	27	theme	SL	1626:1627	arg1	diet					1629:1632	the SL diet	1622:1632	the SL diet	1622:1632	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	15	28	theme	linoleic	2097:2104	arg1	acid					2106:2109	dietary linoleic acid	2089:2109	dietary linoleic acid	2089:2109	Compared to the FR diet, the SL diet led to a greater omega-3 fatty acid content because of a greater supply of dietary linoleic acid, especially in lean muscle.
25412752	9	29	theme	fattening	1322:1330	arg1	performance					1332:1342	greater fattening performance	1314:1342	greater fattening performance	1314:1342	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	4	30	theme	SLR	778:780	arg1	diet					782:785	the SLR diet	774:785	the SLR diet	774:785	The concentrate mix was offered ad libitum in SL and FR diets but was kept isoenergetic to the FR diet in the SLR diet.
25412752	0	31	theme	fiber-rich	81:90	arg1	diets					134:138	fiber-rich versus starch-plus-lipid-rich concentrate diets	81:138	fiber-rich versus starch-plus-lipid-rich concentrate diets	81:138	Fattening performance, metabolic indicators, and muscle composition of bulls fed fiber-rich versus starch-plus-lipid-rich concentrate diets.
25412752	10	32	theme	heavier	1472:1478	arg1	carcasses					1480:1488	heavier carcasses	1472:1488	heavier carcasses	1472:1488	They also had heavier carcasses with a greater proportion of fat.
25412752	13	33	contain	had	1770:1772	arg2	ratio					1823:1827	a greater muscle-to-fat ratio	1799:1827	a greater muscle-to-fat ratio	1799:1827	They had lower carcass weight but a greater muscle-to-fat ratio.
25412752	13	33	contain	had	1770:1772	arg1	They					1765:1768	They	1765:1768	They	1765:1768	They had lower carcass weight but a greater muscle-to-fat ratio.
25412752	13	33	contain	had	1770:1772	arg2	weight					1788:1793	lower carcass weight	1774:1793	lower carcass weight	1774:1793	They had lower carcass weight but a greater muscle-to-fat ratio.
25412752	8	34	theme	Metabolic	1151:1159	arg1	activity					1168:1175	Metabolic enzyme activity	1151:1175	Metabolic enzyme activity (phosphofructokinase, lactate dehydrogenase, and cytochrome-c oxidase)	1151:1246	Metabolic enzyme activity (phosphofructokinase, lactate dehydrogenase, and cytochrome-c oxidase) was measured on these muscles and on liver.
25412752	14	35	theme	planned	1890:1896	arg1	intake					1902:1907	planned NEg intake	1890:1907	planned NEg intake	1890:1907	Compared to bulls fed the FR diet, SLR bulls had lower than planned NEg intake and lower BW gain but did not have differences in body composition.
25412752	7	36	theme	fatty	986:990	arg1	composition					997:1007	fatty acid composition	986:1007	fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids	986:1059	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	15	37	theme	greater	2071:2077	arg1	supply					2079:2084	a greater supply	2069:2084	a greater supply of dietary linoleic acid	2069:2109	Compared to the FR diet, the SL diet led to a greater omega-3 fatty acid content because of a greater supply of dietary linoleic acid, especially in lean muscle.
25412752	11	38	from	difference	1557:1566	arg1	activity					1590:1597	specific metabolic activity	1571:1597	specific metabolic activity	1571:1597	However, liver samples showed no difference in specific metabolic activity.
25412752	2	39	theme	Charolais	370:378	arg1	bulls					380:384	140 Charolais bulls	366:384	140 Charolais bulls (initially 319 ± 27 kg BW)	366:411	A total of 140 Charolais bulls (initially 319 ± 27 kg BW) were allocated to 3 high-concentrate diets and were fattened for up to 18 mo.
25412752	2	39	theme	Charolais	370:378	arg1	BW					409:410	initially 319 ± 27 kg BW	387:410	initially 319 ± 27 kg BW	387:410	A total of 140 Charolais bulls (initially 319 ± 27 kg BW) were allocated to 3 high-concentrate diets and were fattened for up to 18 mo.
25412752	0	40	theme	Fattening	0:8	arg1	performance					10:20	Fattening performance	0:20	Fattening performance	0:20	Fattening performance, metabolic indicators, and muscle composition of bulls fed fiber-rich versus starch-plus-lipid-rich concentrate diets.
25412752	3	41	dep	diets	599:603	arg1	diets					599:603	diets SL and SLR	599:614	diets SL and SLR	599:614	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	3	41	dep	diets	599:603	arg1	SLR					612:614	SLR	612:614	SLR	612:614	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	3	41	dep	diets	599:603	arg1	SL					605:606	SL	605:606	SL	605:606	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	15	42	theme	omega-3	2031:2037	arg1	acid					2045:2048	a greater omega-3 fatty acid	2021:2048	a greater omega-3 fatty acid content	2021:2056	Compared to the FR diet, the SL diet led to a greater omega-3 fatty acid content because of a greater supply of dietary linoleic acid, especially in lean muscle.
25412752	2	43	theme	high-concentrate	433:448	arg1	diets					450:454	3 high-concentrate diets	431:454	3 high-concentrate diets	431:454	A total of 140 Charolais bulls (initially 319 ± 27 kg BW) were allocated to 3 high-concentrate diets and were fattened for up to 18 mo.
25412752	8	44	theme	lactate	1199:1205	arg1	dehydrogenase					1207:1219	lactate dehydrogenase	1199:1219	lactate dehydrogenase	1199:1219	Metabolic enzyme activity (phosphofructokinase, lactate dehydrogenase, and cytochrome-c oxidase) was measured on these muscles and on liver.
25412752	3	45	from	fiber	556:560	arg1	rich					541:544	rich	541:544	rich	541:544	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	1	46	from	by-products	342:352	arg1	rich					265:268	rich	265:268	rich	265:268	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	6	47	theme	Carcass	851:857	arg1	composition					859:869	Carcass composition	851:869	Carcass composition	851:869	Carcass composition was assessed for all animals slaughtered at 699 ± 65 kg BW.
25412752	7	48	theme	quality	948:954	arg1	traits					956:961	Meat nutritional quality traits	931:961	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids)	931:1060	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	3	49	dep	=	619:619	arg1	diets					599:603	diets SL and SLR	599:614	diets SL and SLR	599:614	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	3	49	dep	=	619:619	arg1	SLR					612:614	SLR	612:614	SLR	612:614	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	3	49	dep	=	619:619	arg1	SL					605:606	SL	605:606	SL	605:606	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	9	50	theme	energy	1410:1415	arg1	intake					1417:1422	an energy intake	1407:1422	an energy intake similar to that of FR diet bulls	1407:1455	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	9	51	contain	had	1310:1312	arg2	P					1393:1393	P = 0.025	1393:1401	P = 0.025	1393:1401	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	9	51	contain	had	1310:1312	arg2	performance					1332:1342	greater fattening performance	1314:1342	greater fattening performance	1314:1342	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	9	51	contain	had	1310:1312	arg2	gain					1348:1351	BW gain	1345:1351	BW gain (P = 0.006)	1345:1363	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	9	51	contain	had	1310:1312	arg2	P					1354:1354	P = 0.006	1354:1362	P = 0.006	1354:1362	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	9	51	contain	had	1310:1312	arg2	efficiency					1370:1379	efficiency	1370:1379	efficiency for growth (P = 0.025)	1370:1402	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	9	51	contain	had	1310:1312	arg1	bulls					1304:1308	The SL diet bulls	1292:1308	The SL diet bulls	1292:1308	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	8	52	theme	cytochrome-c	1226:1237	arg1	oxidase					1239:1245	cytochrome-c oxidase	1226:1245	cytochrome-c oxidase	1226:1245	Metabolic enzyme activity (phosphofructokinase, lactate dehydrogenase, and cytochrome-c oxidase) was measured on these muscles and on liver.
25412752	1	53	theme	meat	221:224	arg1	composition					226:236	meat composition	221:236	meat composition	221:236	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	5	54	theme	d.	816:817	arg1	intake					824:829	every 15 d. Feed intake	807:829	every 15 d. Feed intake	807:829	Bulls were weighed every 15 d. Feed intake was measured daily.
25412752	7	55	theme	longissimus	1083:1093	arg1	thoracis					1095:1102	the longissimus thoracis	1079:1102	the longissimus thoracis	1079:1102	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	13	56	theme	muscle-to-fat	1809:1821	arg1	ratio					1823:1827	a greater muscle-to-fat ratio	1799:1827	a greater muscle-to-fat ratio	1799:1827	They had lower carcass weight but a greater muscle-to-fat ratio.
25412752	7	57	theme	nutritional	936:946	arg1	traits					956:961	Meat nutritional quality traits	931:961	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids)	931:1060	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	11	58	theme	metabolic	1580:1588	arg1	activity					1590:1597	specific metabolic activity	1571:1597	specific metabolic activity	1571:1597	However, liver samples showed no difference in specific metabolic activity.
25412752	15	59	theme	lean	2126:2129	arg1	muscle					2131:2136	lean muscle	2126:2136	lean muscle	2126:2136	Compared to the FR diet, the SL diet led to a greater omega-3 fatty acid content because of a greater supply of dietary linoleic acid, especially in lean muscle.
25412752	3	60	dep	starch	578:583	arg1	=					619:619	=	619:619	=	619:619	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	7	61	theme	polyunsaturated	1033:1047	arg1	acids					1055:1059	n-6 and n-3 polyunsaturated fatty acids	1021:1059	acids	1055:1059	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	9	62	theme	greater	1314:1320	arg1	performance					1332:1342	greater fattening performance	1314:1342	greater fattening performance	1314:1342	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	1	63	from	lipids	291:296	arg1	rich					265:268	rich	265:268	rich	265:268	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	12	64	contain	had	1678:1680	arg2	<					1699:1699	P < 0.001	1697:1705	P < 0.001	1697:1705	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	12	64	contain	had	1678:1680	arg2	gain					1691:1694	lower BW gain	1682:1694	lower BW gain (P < 0.001)	1682:1706	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	12	64	contain	had	1678:1680	arg1	bulls					1635:1639	bulls	1635:1639	bulls fed SLR	1635:1647	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	12	65	theme	BW	1688:1689	arg1	gain					1691:1694	lower BW gain	1682:1694	lower BW gain (P < 0.001)	1682:1706	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	12	65	theme	BW	1688:1689	arg1	<					1699:1699	P < 0.001	1697:1705	P < 0.001	1697:1705	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	4	66	theme	ad	700:701	arg1	libitum					703:709	ad libitum	700:709	ad libitum	700:709	The concentrate mix was offered ad libitum in SL and FR diets but was kept isoenergetic to the FR diet in the SLR diet.
25412752	1	67	theme	fatty	318:322	arg1	acids					324:328	omega-3 fatty acids	310:328	especially omega-3 fatty acids	299:328	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	1	68	from	starch	280:285	arg1	rich					265:268	rich	265:268	rich	265:268	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	3	69	theme	concentrate	520:530	arg1	mixtures					532:539	concentrate mixtures	520:539	concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw	520:665	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	8	70	dep	activity	1168:1175	arg1	dehydrogenase					1207:1219	lactate dehydrogenase	1199:1219	lactate dehydrogenase	1199:1219	Metabolic enzyme activity (phosphofructokinase, lactate dehydrogenase, and cytochrome-c oxidase) was measured on these muscles and on liver.
25412752	8	70	dep	activity	1168:1175	arg1	oxidase					1239:1245	cytochrome-c oxidase	1226:1245	cytochrome-c oxidase	1226:1245	Metabolic enzyme activity (phosphofructokinase, lactate dehydrogenase, and cytochrome-c oxidase) was measured on these muscles and on liver.
25412752	8	70	dep	activity	1168:1175	arg1	phosphofructokinase					1178:1196	phosphofructokinase	1178:1196	phosphofructokinase	1178:1196	Metabolic enzyme activity (phosphofructokinase, lactate dehydrogenase, and cytochrome-c oxidase) was measured on these muscles and on liver.
25412752	9	71	theme	similar	1424:1430	arg1	intake					1417:1422	an energy intake	1407:1422	an energy intake similar to that of FR diet bulls	1407:1455	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	4	72	from	diet	766:769	arg1	diet					782:785	the SLR diet	774:785	the SLR diet	774:785	The concentrate mix was offered ad libitum in SL and FR diets but was kept isoenergetic to the FR diet in the SLR diet.
25412752	9	73	theme	FR	1443:1444	arg1	bulls					1451:1455	FR diet bulls	1443:1455	FR diet bulls	1443:1455	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	12	74	theme	=	1755:1755	arg1	0.010					1757:1761	P = 0.010	1753:1761	P = 0.010	1753:1761	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	12	74	theme	=	1755:1755	arg1	growth					1745:1750	growth	1745:1750	growth (P = 0.010)	1745:1762	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	10	75	theme	fat	1519:1521	arg1	proportion					1505:1514	a greater proportion	1495:1514	a greater proportion of fat	1495:1521	They also had heavier carcasses with a greater proportion of fat.
25412752	13	76	theme	carcass	1780:1786	arg1	weight					1788:1793	lower carcass weight	1774:1793	lower carcass weight	1774:1793	They had lower carcass weight but a greater muscle-to-fat ratio.
25412752	11	77	theme	liver	1533:1537	arg1	samples					1539:1545	liver samples	1533:1545	liver samples	1533:1545	However, liver samples showed no difference in specific metabolic activity.
25412752	15	78	theme	acid	2045:2048	arg1	content					2050:2056	a greater omega-3 fatty acid content	2021:2056	a greater omega-3 fatty acid content	2021:2056	Compared to the FR diet, the SL diet led to a greater omega-3 fatty acid content because of a greater supply of dietary linoleic acid, especially in lean muscle.
25412752	9	79	theme	SL	1296:1297	arg1	bulls					1304:1308	The SL diet bulls	1292:1308	The SL diet bulls	1292:1308	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	7	80	theme	n-6	1021:1023	arg1	acids					1055:1059	n-6 and n-3 polyunsaturated fatty acids	1021:1059	acids	1055:1059	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	1	81	from	responses	182:190	arg1	performance					205:215	fattening performance	195:215	fattening performance	195:215	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	1	81	from	responses	182:190	arg1	composition					226:236	meat composition	221:236	meat composition	221:236	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	10	82	contain	had	1468:1470	arg2	carcasses					1480:1488	heavier carcasses	1472:1488	heavier carcasses	1472:1488	They also had heavier carcasses with a greater proportion of fat.
25412752	10	82	contain	had	1468:1470	arg1	They					1458:1461	They	1458:1461	They	1458:1461	They also had heavier carcasses with a greater proportion of fat.
25412752	3	83	dep	=	569:569	arg1	FR					563:564	FR	563:564	FR	563:564	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	14	84	theme	BW	1919:1920	arg1	gain					1922:1925	lower BW gain	1913:1925	lower BW gain	1913:1925	Compared to bulls fed the FR diet, SLR bulls had lower than planned NEg intake and lower BW gain but did not have differences in body composition.
25412752	1	85	theme	study	157:161	arg1	aim					145:147	The aim	141:147	The aim of this study	141:161	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	1	86	dep	starch	280:285	arg1	acids					324:328	omega-3 fatty acids	310:328	especially omega-3 fatty acids	299:328	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	7	87	theme	acid	992:995	arg1	composition					997:1007	fatty acid composition	986:1007	fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids	986:1059	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	15	88	theme	SL	2006:2007	arg1	diet					2009:2012	the SL diet	2002:2012	the SL diet	2002:2012	Compared to the FR diet, the SL diet led to a greater omega-3 fatty acid content because of a greater supply of dietary linoleic acid, especially in lean muscle.
25412752	15	89	theme	acid	2106:2109	arg1	supply					2079:2084	a greater supply	2069:2084	a greater supply of dietary linoleic acid	2069:2109	Compared to the FR diet, the SL diet led to a greater omega-3 fatty acid content because of a greater supply of dietary linoleic acid, especially in lean muscle.
25412752	7	90	dep	content	974:980	arg1	e.g.					964:967	e.g.	964:967	e.g.	964:967	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	14	91	theme	NEg	1898:1900	arg1	intake					1902:1907	planned NEg intake	1890:1907	planned NEg intake	1890:1907	Compared to bulls fed the FR diet, SLR bulls had lower than planned NEg intake and lower BW gain but did not have differences in body composition.
25412752	0	92	theme	concentrate	122:132	arg1	diets					134:138	fiber-rich versus starch-plus-lipid-rich concentrate diets	81:138	fiber-rich versus starch-plus-lipid-rich concentrate diets	81:138	Fattening performance, metabolic indicators, and muscle composition of bulls fed fiber-rich versus starch-plus-lipid-rich concentrate diets.
25412752	15	93	theme	dietary	2089:2095	arg1	acid					2106:2109	dietary linoleic acid	2089:2109	dietary linoleic acid	2089:2109	Compared to the FR diet, the SL diet led to a greater omega-3 fatty acid content because of a greater supply of dietary linoleic acid, especially in lean muscle.
25412752	9	94	theme	BW	1345:1346	arg1	P					1354:1354	P = 0.006	1354:1362	P = 0.006	1354:1362	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	9	94	theme	BW	1345:1346	arg1	gain					1348:1351	BW gain	1345:1351	BW gain (P = 0.006)	1345:1363	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	2	95	theme	bulls	380:384	arg1	total					357:361	A total	355:361	A total of 140 Charolais bulls (initially 319 ± 27 kg BW)	355:411	A total of 140 Charolais bulls (initially 319 ± 27 kg BW) were allocated to 3 high-concentrate diets and were fattened for up to 18 mo.
25412752	4	96	dep	SL	714:715	arg1	diets					724:728	diets	724:728	diets	724:728	The concentrate mix was offered ad libitum in SL and FR diets but was kept isoenergetic to the FR diet in the SLR diet.
25412752	15	97	theme	FR	1993:1994	arg1	diet					1996:1999	the FR diet	1989:1999	the FR diet	1989:1999	Compared to the FR diet, the SL diet led to a greater omega-3 fatty acid content because of a greater supply of dietary linoleic acid, especially in lean muscle.
25412752	7	98	theme	rectus	1105:1110	arg1	abdominis					1112:1120	rectus abdominis	1105:1120	rectus abdominis	1105:1120	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	8	99	theme	enzyme	1161:1166	arg1	activity					1168:1175	Metabolic enzyme activity	1151:1175	Metabolic enzyme activity (phosphofructokinase, lactate dehydrogenase, and cytochrome-c oxidase)	1151:1246	Metabolic enzyme activity (phosphofructokinase, lactate dehydrogenase, and cytochrome-c oxidase) was measured on these muscles and on liver.
25412752	3	100	from	rich	541:544	arg1	fiber					556:560	fiber	556:560	fiber (FR; n = 56)	556:573	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	3	100	from	rich	541:544	arg1	straw					661:665	starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw	578:665	starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw	578:665	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	0	101	theme	metabolic	23:31	arg1	indicators					33:42	metabolic indicators	23:42	metabolic indicators	23:42	Fattening performance, metabolic indicators, and muscle composition of bulls fed fiber-rich versus starch-plus-lipid-rich concentrate diets.
25412752	9	102	theme	=	1356:1356	arg1	P					1354:1354	P = 0.006	1354:1362	P = 0.006	1354:1362	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	9	102	theme	=	1356:1356	arg1	gain					1348:1351	BW gain	1345:1351	BW gain (P = 0.006)	1345:1363	The SL diet bulls had greater fattening performance, BW gain (P = 0.006), and efficiency for growth (P = 0.025) at an energy intake similar to that of FR diet bulls.
25412752	14	103	theme	SLR	1865:1867	arg1	bulls					1869:1873	SLR bulls	1865:1873	SLR bulls	1865:1873	Compared to bulls fed the FR diet, SLR bulls had lower than planned NEg intake and lower BW gain but did not have differences in body composition.
25412752	2	104	dep	18	484:485	arg1	to					481:482	to	481:482	to	481:482	A total of 140 Charolais bulls (initially 319 ± 27 kg BW) were allocated to 3 high-concentrate diets and were fattened for up to 18 mo.
25412752	4	105	theme	concentrate	672:682	arg1	isoenergetic					743:754	isoenergetic	743:754	isoenergetic	743:754	The concentrate mix was offered ad libitum in SL and FR diets but was kept isoenergetic to the FR diet in the SLR diet.
25412752	4	105	theme	concentrate	672:682	arg1	mix					684:686	The concentrate mix	668:686	The concentrate mix	668:686	The concentrate mix was offered ad libitum in SL and FR diets but was kept isoenergetic to the FR diet in the SLR diet.
25412752	0	106	theme	starch-plus-lipid-rich	99:120	arg1	diets					134:138	fiber-rich versus starch-plus-lipid-rich concentrate diets	81:138	fiber-rich versus starch-plus-lipid-rich concentrate diets	81:138	Fattening performance, metabolic indicators, and muscle composition of bulls fed fiber-rich versus starch-plus-lipid-rich concentrate diets.
25412752	0	107	theme	muscle	49:54	arg1	composition					56:66	muscle composition	49:66	muscle composition	49:66	Fattening performance, metabolic indicators, and muscle composition of bulls fed fiber-rich versus starch-plus-lipid-rich concentrate diets.
25412752	1	108	theme	omega-3	310:316	arg1	acids					324:328	omega-3 fatty acids	310:328	especially omega-3 fatty acids	299:328	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
25412752	3	109	from	straw	661:665	arg1	rich					541:544	rich	541:544	rich	541:544	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	3	110	theme	linseed	590:596	arg1	straw					661:665	starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw	578:665	starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw	578:665	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	11	111	theme	specific	1571:1578	arg1	activity					1590:1597	specific metabolic activity	1571:1597	specific metabolic activity	1571:1597	However, liver samples showed no difference in specific metabolic activity.
25412752	3	112	theme	starch	578:583	arg1	straw					661:665	starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw	578:665	starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw	578:665	The diet treatments included concentrate mixtures rich in either fiber (FR; n = 56) or starch plus linseed (diets SL and SLR; n = 56 and n = 28, respectively) and barley straw.
25412752	13	113	theme	greater	1801:1807	arg1	ratio					1823:1827	a greater muscle-to-fat ratio	1799:1827	a greater muscle-to-fat ratio	1799:1827	They had lower carcass weight but a greater muscle-to-fat ratio.
25412752	12	114	theme	P	1697:1697	arg1	gain					1691:1694	lower BW gain	1682:1694	lower BW gain (P < 0.001)	1682:1706	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	12	114	theme	P	1697:1697	arg1	<					1699:1699	P < 0.001	1697:1705	P < 0.001	1697:1705	Compared to bulls fed the SL diet, bulls fed SLR consumed 15% less energy and had lower BW gain (P < 0.001) but were slightly more efficient for growth (P = 0.010).
25412752	5	115	theme	Feed	819:822	arg1	intake					824:829	every 15 d. Feed intake	807:829	every 15 d. Feed intake	807:829	Bulls were weighed every 15 d. Feed intake was measured daily.
25412752	7	116	theme	fatty	1049:1053	arg1	acids					1055:1059	n-6 and n-3 polyunsaturated fatty acids	1021:1059	acids	1055:1059	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	14	117	theme	FR	1856:1857	arg1	diet					1859:1862	the FR diet	1852:1862	the FR diet	1852:1862	Compared to bulls fed the FR diet, SLR bulls had lower than planned NEg intake and lower BW gain but did not have differences in body composition.
25412752	7	118	theme	fat	970:972	arg1	content					974:980	fat content	970:980	fat content	970:980	Meat nutritional quality traits (e.g., fat content and fatty acid composition focusing on n-6 and n-3 polyunsaturated fatty acids) were measured on the longissimus thoracis, rectus abdominis, and semitendinosus muscles.
25412752	1	119	theme	high-concentrate	242:257	arg1	diets					259:263	high-concentrate diets	242:263	high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products	242:352	The aim of this study was to compare the responses in fattening performance and meat composition for high-concentrate diets rich in either starch and lipids (especially omega-3 fatty acids) or fibrous by-products.
26304787	8	0	theme	fibrolytic	1042:1051	arg1	bacteria					1053:1060	fibrolytic bacteria	1042:1060	fibrolytic bacteria	1042:1060	We monitored rumen microbial community composition in Experiment 2 and found increases in fibrolytic bacteria and methanogenic archaea in CE calves, but the overall microbial composition did not differ between the groups.
26304787	9	1	theme	growth	1245:1250	arg1	performance					1252:1262	growth performance	1245:1262	growth performance in weaned calves on pasture	1245:1290	Our results suggest that supplementation with CE may positively affect growth performance in weaned calves on pasture, but longer-term CE supplementation may be required to exhibit the effect.
26304787	4	2	theme	Black	475:479	arg1	calves					481:486	Eight Japanese Black calves	460:486	Eight Japanese Black calves	460:486	Eight Japanese Black calves were assigned to either a control group (CON) or an experimental group (CE) fed CE at a rate of 10 g/day mixed with concentrate.
26304787	9	3	theme	CE	1309:1310	arg1	supplementation					1312:1326	longer-term CE supplementation	1297:1326	longer-term CE supplementation	1297:1326	Our results suggest that supplementation with CE may positively affect growth performance in weaned calves on pasture, but longer-term CE supplementation may be required to exhibit the effect.
26304787	0	4	theme	calves	86:91	arg1	performance					64:74	growth performance	57:74	growth performance of weaned calves on pasture	57:102	Effects of supplementation with cellooligosaccharides on growth performance of weaned calves on pasture.
26304787	6	5	theme	significant	818:828	arg1	differences					830:840	no significant differences	815:840	no significant differences in body weight changes	815:863	In both experiments, average daily gain tended to be greater in CE than in CON, especially in late stages of experiments, but no significant differences in body weight changes were observed.
26304787	1	6	from	effects	158:164	arg1	performance					221:231	growth performance	214:231	growth performance in grazing beef calves	214:254	We conducted two feeding experiments to evaluate the effects of cellooligosaccharide (CE) supplementation on growth performance in grazing beef calves.
26304787	0	7	from	Effects	0:6	arg1	performance					64:74	growth performance	57:74	growth performance of weaned calves on pasture	57:102	Effects of supplementation with cellooligosaccharides on growth performance of weaned calves on pasture.
26304787	8	8	theme	overall	1109:1115	arg1	composition					1127:1137	the overall microbial composition	1105:1137	the overall microbial composition	1105:1137	We monitored rumen microbial community composition in Experiment 2 and found increases in fibrolytic bacteria and methanogenic archaea in CE calves, but the overall microbial composition did not differ between the groups.
26304787	0	9	from	performance	64:74	arg1	pasture					96:102	pasture	96:102	pasture	96:102	Effects of supplementation with cellooligosaccharides on growth performance of weaned calves on pasture.
26304787	0	10	with	supplementation	11:25	arg1	cellooligosaccharides					32:52	cellooligosaccharides	32:52	cellooligosaccharides	32:52	Effects of supplementation with cellooligosaccharides on growth performance of weaned calves on pasture.
26304787	9	11	theme	weaned	1267:1272	arg1	calves					1274:1279	weaned calves	1267:1279	weaned calves	1267:1279	Our results suggest that supplementation with CE may positively affect growth performance in weaned calves on pasture, but longer-term CE supplementation may be required to exhibit the effect.
26304787	4	12	theme	Japanese	466:473	arg1	calves					481:486	Eight Japanese Black calves	460:486	Eight Japanese Black calves	460:486	Eight Japanese Black calves were assigned to either a control group (CON) or an experimental group (CE) fed CE at a rate of 10 g/day mixed with concentrate.
26304787	3	13	used	used	432:435	arg2	Experiment					408:417	Experiment 2	408:419	Experiment 2 (13 weeks)	408:430	Experiment 1 (10 weeks) used 5.6-month-old castrated males; Experiment 2 (13 weeks) used 3.9-month-old females.
26304787	3	13	used	used	432:435	arg2	13 weeks					422:429	13 weeks	422:429	13 weeks	422:429	Experiment 1 (10 weeks) used 5.6-month-old castrated males; Experiment 2 (13 weeks) used 3.9-month-old females.
26304787	1	14	theme	cellooligosaccharide	169:188	arg1	supplementation					195:209	cellooligosaccharide (CE) supplementation	169:209	cellooligosaccharide (CE) supplementation	169:209	We conducted two feeding experiments to evaluate the effects of cellooligosaccharide (CE) supplementation on growth performance in grazing beef calves.
26304787	6	15	from	CON	764:766	arg1	stages					788:793	late stages	783:793	late stages of experiments	783:808	In both experiments, average daily gain tended to be greater in CE than in CON, especially in late stages of experiments, but no significant differences in body weight changes were observed.
26304787	6	16	theme	experiments	798:808	arg1	stages					788:793	late stages	783:793	late stages of experiments	783:808	In both experiments, average daily gain tended to be greater in CE than in CON, especially in late stages of experiments, but no significant differences in body weight changes were observed.
26304787	9	17	from	performance	1252:1262	arg1	pasture					1284:1290	pasture	1284:1290	pasture	1284:1290	Our results suggest that supplementation with CE may positively affect growth performance in weaned calves on pasture, but longer-term CE supplementation may be required to exhibit the effect.
26304787	9	17	from	performance	1252:1262	arg1	calves					1274:1279	weaned calves	1267:1279	weaned calves	1267:1279	Our results suggest that supplementation with CE may positively affect growth performance in weaned calves on pasture, but longer-term CE supplementation may be required to exhibit the effect.
26304787	5	18	from	fields	640:645	arg1	dominant					679:686	dominant	679:686	dominant	679:686	Calves were stocked in fields in which Kentucky bluegrass was dominant.
26304787	3	19	theme	5.6-month-old	377:389	arg1	males					401:405	5.6-month-old castrated males	377:405	5.6-month-old castrated males	377:405	Experiment 1 (10 weeks) used 5.6-month-old castrated males; Experiment 2 (13 weeks) used 3.9-month-old females.
26304787	6	20	from	differences	830:840	arg1	changes					857:863	body weight changes	845:863	body weight changes	845:863	In both experiments, average daily gain tended to be greater in CE than in CON, especially in late stages of experiments, but no significant differences in body weight changes were observed.
26304787	8	21	theme	microbial	971:979	arg1	composition					991:1001	rumen microbial community composition	965:1001	rumen microbial community composition	965:1001	We monitored rumen microbial community composition in Experiment 2 and found increases in fibrolytic bacteria and methanogenic archaea in CE calves, but the overall microbial composition did not differ between the groups.
26304787	1	22	theme	CE	191:192	arg1	supplementation					195:209	cellooligosaccharide (CE) supplementation	169:209	cellooligosaccharide (CE) supplementation	169:209	We conducted two feeding experiments to evaluate the effects of cellooligosaccharide (CE) supplementation on growth performance in grazing beef calves.
26304787	3	23	theme	castrated	391:399	arg1	males					401:405	5.6-month-old castrated males	377:405	5.6-month-old castrated males	377:405	Experiment 1 (10 weeks) used 5.6-month-old castrated males; Experiment 2 (13 weeks) used 3.9-month-old females.
26304787	2	24	theme	period	307:312	arg1	duration					278:285	duration	278:285	duration	278:285	Calf sex and age and duration of the experimental period differed between the experiments.
26304787	2	24	theme	period	307:312	arg1	age					270:272	age	270:272	age	270:272	Calf sex and age and duration of the experimental period differed between the experiments.
26304787	2	24	theme	period	307:312	arg1	sex					262:264	Calf sex	257:264	Calf sex	257:264	Calf sex and age and duration of the experimental period differed between the experiments.
26304787	6	25	theme	late	783:786	arg1	stages					788:793	late stages	783:793	late stages of experiments	783:808	In both experiments, average daily gain tended to be greater in CE than in CON, especially in late stages of experiments, but no significant differences in body weight changes were observed.
26304787	8	26	theme	methanogenic	1066:1077	arg1	archaea					1079:1085	methanogenic archaea	1066:1085	methanogenic archaea	1066:1085	We monitored rumen microbial community composition in Experiment 2 and found increases in fibrolytic bacteria and methanogenic archaea in CE calves, but the overall microbial composition did not differ between the groups.
26304787	3	27	used	used	372:375	arg2	10 weeks					362:369	10 weeks	362:369	10 weeks	362:369	Experiment 1 (10 weeks) used 5.6-month-old castrated males; Experiment 2 (13 weeks) used 3.9-month-old females.
26304787	3	27	used	used	372:375	arg2	Experiment					348:357	Experiment 1	348:359	Experiment 1 (10 weeks)	348:370	Experiment 1 (10 weeks) used 5.6-month-old castrated males; Experiment 2 (13 weeks) used 3.9-month-old females.
26304787	6	28	theme	daily	718:722	arg1	gain					724:727	average daily gain	710:727	average daily gain	710:727	In both experiments, average daily gain tended to be greater in CE than in CON, especially in late stages of experiments, but no significant differences in body weight changes were observed.
26304787	2	29	theme	experimental	294:305	arg1	period					307:312	the experimental period	290:312	the experimental period	290:312	Calf sex and age and duration of the experimental period differed between the experiments.
26304787	1	30	theme	supplementation	195:209	arg1	effects					158:164	the effects	154:164	the effects of cellooligosaccharide (CE) supplementation on growth performance in grazing beef calves	154:254	We conducted two feeding experiments to evaluate the effects of cellooligosaccharide (CE) supplementation on growth performance in grazing beef calves.
26304787	0	31	theme	supplementation	11:25	arg1	Effects					0:6	Effects	0:6	Effects of supplementation with cellooligosaccharides on growth performance of weaned calves on pasture.	0:103	Effects of supplementation with cellooligosaccharides on growth performance of weaned calves on pasture.
26304787	4	32	theme	experimental	540:551	arg1	CE					560:561	CE	560:561	CE	560:561	Eight Japanese Black calves were assigned to either a control group (CON) or an experimental group (CE) fed CE at a rate of 10 g/day mixed with concentrate.
26304787	4	32	theme	experimental	540:551	arg1	group					553:557	an experimental group	537:557	an experimental group (CE) fed CE at a rate of 10 g/day mixed with concentrate	537:614	Eight Japanese Black calves were assigned to either a control group (CON) or an experimental group (CE) fed CE at a rate of 10 g/day mixed with concentrate.
26304787	8	33	theme	rumen	965:969	arg1	composition					991:1001	rumen microbial community composition	965:1001	rumen microbial community composition	965:1001	We monitored rumen microbial community composition in Experiment 2 and found increases in fibrolytic bacteria and methanogenic archaea in CE calves, but the overall microbial composition did not differ between the groups.
26304787	5	34	theme	Kentucky	656:663	arg1	bluegrass					665:673	Kentucky bluegrass	656:673	Kentucky bluegrass	656:673	Calves were stocked in fields in which Kentucky bluegrass was dominant.
26304787	3	35	theme	3.9-month-old	437:449	arg1	females					451:457	3.9-month-old females	437:457	3.9-month-old females	437:457	Experiment 1 (10 weeks) used 5.6-month-old castrated males; Experiment 2 (13 weeks) used 3.9-month-old females.
26304787	4	36	from	rate	576:579	arg1	CE					568:569	CE	568:569	CE at a rate of 10 g/day mixed with concentrate	568:614	Eight Japanese Black calves were assigned to either a control group (CON) or an experimental group (CE) fed CE at a rate of 10 g/day mixed with concentrate.
26304787	1	37	theme	growth	214:219	arg1	performance					221:231	growth performance	214:231	growth performance in grazing beef calves	214:254	We conducted two feeding experiments to evaluate the effects of cellooligosaccharide (CE) supplementation on growth performance in grazing beef calves.
26304787	8	38	theme	community	981:989	arg1	composition					991:1001	rumen microbial community composition	965:1001	rumen microbial community composition	965:1001	We monitored rumen microbial community composition in Experiment 2 and found increases in fibrolytic bacteria and methanogenic archaea in CE calves, but the overall microbial composition did not differ between the groups.
26304787	8	39	from	increases	1029:1037	arg1	bacteria					1053:1060	fibrolytic bacteria	1042:1060	fibrolytic bacteria	1042:1060	We monitored rumen microbial community composition in Experiment 2 and found increases in fibrolytic bacteria and methanogenic archaea in CE calves, but the overall microbial composition did not differ between the groups.
26304787	8	39	from	increases	1029:1037	arg1	archaea					1079:1085	methanogenic archaea	1066:1085	methanogenic archaea	1066:1085	We monitored rumen microbial community composition in Experiment 2 and found increases in fibrolytic bacteria and methanogenic archaea in CE calves, but the overall microbial composition did not differ between the groups.
26304787	6	40	theme	average	710:716	arg1	gain					724:727	average daily gain	710:727	average daily gain	710:727	In both experiments, average daily gain tended to be greater in CE than in CON, especially in late stages of experiments, but no significant differences in body weight changes were observed.
26304787	4	41	theme	10 g/day	584:591	arg1	rate					576:579	a rate	574:579	a rate of 10 g/day mixed with concentrate	574:614	Eight Japanese Black calves were assigned to either a control group (CON) or an experimental group (CE) fed CE at a rate of 10 g/day mixed with concentrate.
26304787	8	42	theme	microbial	1117:1125	arg1	composition					1127:1137	the overall microbial composition	1105:1137	the overall microbial composition	1105:1137	We monitored rumen microbial community composition in Experiment 2 and found increases in fibrolytic bacteria and methanogenic archaea in CE calves, but the overall microbial composition did not differ between the groups.
26304787	4	43	theme	control	514:520	arg1	CON					529:531	CON	529:531	CON	529:531	Eight Japanese Black calves were assigned to either a control group (CON) or an experimental group (CE) fed CE at a rate of 10 g/day mixed with concentrate.
26304787	4	43	theme	control	514:520	arg1	group					522:526	a control group	512:526	a control group (CON)	512:532	Eight Japanese Black calves were assigned to either a control group (CON) or an experimental group (CE) fed CE at a rate of 10 g/day mixed with concentrate.
26304787	5	44	from	dominant	679:686	arg1	fields					640:645	fields	640:645	fields in which Kentucky bluegrass was dominant	640:686	Calves were stocked in fields in which Kentucky bluegrass was dominant.
26304787	1	45	from	performance	221:231	arg1	calves					249:254	grazing beef calves	236:254	grazing beef calves	236:254	We conducted two feeding experiments to evaluate the effects of cellooligosaccharide (CE) supplementation on growth performance in grazing beef calves.
26304787	0	46	theme	growth	57:62	arg1	performance					64:74	growth performance	57:74	growth performance of weaned calves on pasture	57:102	Effects of supplementation with cellooligosaccharides on growth performance of weaned calves on pasture.
26304787	6	47	theme	weight	850:855	arg1	changes					857:863	body weight changes	845:863	body weight changes	845:863	In both experiments, average daily gain tended to be greater in CE than in CON, especially in late stages of experiments, but no significant differences in body weight changes were observed.
26304787	7	48	theme	CON	940:942	arg1	calves					944:949	CON calves	940:949	CON calves	940:949	In Experiment 2, final heart girth was larger in CE than in CON calves.
26304787	1	49	theme	feeding	122:128	arg1	experiments					130:140	two feeding experiments	118:140	two feeding experiments	118:140	We conducted two feeding experiments to evaluate the effects of cellooligosaccharide (CE) supplementation on growth performance in grazing beef calves.
26304787	6	50	theme	body	845:848	arg1	changes					857:863	body weight changes	845:863	body weight changes	845:863	In both experiments, average daily gain tended to be greater in CE than in CON, especially in late stages of experiments, but no significant differences in body weight changes were observed.
26304787	7	51	theme	final	897:901	arg1	girth					909:913	final heart girth	897:913	final heart girth	897:913	In Experiment 2, final heart girth was larger in CE than in CON calves.
26304787	9	52	with	supplementation	1199:1213	arg1	CE					1220:1221	CE	1220:1221	CE	1220:1221	Our results suggest that supplementation with CE may positively affect growth performance in weaned calves on pasture, but longer-term CE supplementation may be required to exhibit the effect.
26304787	1	53	theme	grazing	236:242	arg1	calves					249:254	grazing beef calves	236:254	grazing beef calves	236:254	We conducted two feeding experiments to evaluate the effects of cellooligosaccharide (CE) supplementation on growth performance in grazing beef calves.
26304787	0	54	theme	weaned	79:84	arg1	calves					86:91	weaned calves	79:91	weaned calves	79:91	Effects of supplementation with cellooligosaccharides on growth performance of weaned calves on pasture.
26304787	2	55	theme	Calf	257:260	arg1	sex					262:264	Calf sex	257:264	Calf sex	257:264	Calf sex and age and duration of the experimental period differed between the experiments.
26304787	7	56	theme	heart	903:907	arg1	girth					909:913	final heart girth	897:913	final heart girth	897:913	In Experiment 2, final heart girth was larger in CE than in CON calves.
26304787	1	57	theme	beef	244:247	arg1	calves					249:254	grazing beef calves	236:254	grazing beef calves	236:254	We conducted two feeding experiments to evaluate the effects of cellooligosaccharide (CE) supplementation on growth performance in grazing beef calves.
26304787	8	58	theme	CE	1090:1091	arg1	calves					1093:1098	CE calves	1090:1098	CE calves	1090:1098	We monitored rumen microbial community composition in Experiment 2 and found increases in fibrolytic bacteria and methanogenic archaea in CE calves, but the overall microbial composition did not differ between the groups.
26304787	9	59	theme	longer-term	1297:1307	arg1	supplementation					1312:1326	longer-term CE supplementation	1297:1326	longer-term CE supplementation	1297:1326	Our results suggest that supplementation with CE may positively affect growth performance in weaned calves on pasture, but longer-term CE supplementation may be required to exhibit the effect.
28032196	3	0	theme	Tibetan	666:672	arg1	TP					683:684	TP	683:684	TP	683:684	This novel approach has been tested for a transect at the southeastern Tibetan plateau (TP), where air masses with different isotopic composition overlap.
28032196	3	0	theme	Tibetan	666:672	arg1	plateau					674:680	the southeastern Tibetan plateau	649:680	the southeastern Tibetan plateau (TP)	649:685	This novel approach has been tested for a transect at the southeastern Tibetan plateau (TP), where air masses with different isotopic composition overlap.
28032196	2	1	theme	trajectory	574:583	arg1	modeling					585:592	backward trajectory modeling	565:592	backward trajectory modeling	565:592	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	1	2	theme	terrestrial	273:283	arg1	archives					291:298	terrestrial δ 18O archives	273:298	terrestrial δ 18O archives	273:298	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	4	3	theme	18O	831:833	arg1	values					835:840	monthly precipitation δ 18O values	807:840	monthly precipitation δ 18O values ([Formula: see text])	807:862	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	7	4	theme	tree-ring	1349:1357	arg1	values					1367:1372	tree-ring isotope values	1349:1372	tree-ring isotope values at semi-humid sites	1349:1392	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	6	5	dep	[Formula	1109:1116	arg1	see					1119:1121	see	1119:1121	see text	1119:1126	Here, air package origin [Formula: see text] relations are generally weaker in contrast to our northern located study sites.
28032196	0	6	theme	Air	0:2	arg1	origin					9:14	Air mass origin	0:14	Air mass origin	0:14	Air mass origin signals in δ 18O of tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau.
28032196	1	7	theme	oxygen	162:167	arg1	origins					190:196	stable oxygen isotope source water origins	155:196	stable oxygen isotope source water origins	155:196	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	3	8	theme	different	710:718	arg1	composition					729:739	different isotopic composition	710:739	different isotopic composition	710:739	This novel approach has been tested for a transect at the southeastern Tibetan plateau (TP), where air masses with different isotopic composition overlap.
28032196	6	9	theme	study	1196:1200	arg1	sites					1202:1206	our northern located study sites	1175:1206	our northern located study sites	1175:1206	Here, air package origin [Formula: see text] relations are generally weaker in contrast to our northern located study sites.
28032196	1	10	theme	source	177:182	arg1	origins					190:196	stable oxygen isotope source water origins	155:196	stable oxygen isotope source water origins	155:196	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	2	11	theme	air	531:533	arg1	origins					543:549	mean annual air package origins	519:549	mean annual air package origins	519:549	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	6	12	theme	[Formula	1109:1116	arg1	relations					1129:1137	air package origin [Formula: see text] relations	1090:1137	air package origin [Formula: see text] relations	1090:1137	Here, air package origin [Formula: see text] relations are generally weaker in contrast to our northern located study sites.
28032196	0	13	theme	monsoonal	100:108	arg1	plateau					118:124	the monsoonal Tibetan plateau	96:124	the monsoonal Tibetan plateau	96:124	Air mass origin signals in δ 18O of tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau.
28032196	1	14	theme	origins	190:196	arg1	precondition					203:214	a precondition	201:214	a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives	201:298	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	1	14	theme	origins	190:196	arg1	consideration					138:150	A profound consideration	127:150	A profound consideration of stable oxygen isotope source water origins	127:196	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	4	15	theme	Interim	892:898	arg1	data					978:981	the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data	884:981	the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data	884:981	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	4	15	theme	Interim	892:898	arg1	LMDZiso					969:975	LMDZiso	969:975	LMDZiso	969:975	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	6	16	theme	located	1188:1194	arg1	sites					1202:1206	our northern located study sites	1175:1206	our northern located study sites	1175:1206	Here, air package origin [Formula: see text] relations are generally weaker in contrast to our northern located study sites.
28032196	7	17	theme	tree-ring	1223:1231	arg1	signatures					1241:1250	tree-ring isotope signatures	1223:1250	tree-ring isotope signatures at dry sites with less rain days per year	1223:1292	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	5	18	theme	study	1028:1032	arg1	sites					1034:1038	Particularly the southernmost study sites	998:1038	Particularly the southernmost study sites	998:1038	Particularly the southernmost study sites are influenced by a distinct amount effect.
28032196	4	19	theme	General	942:948	arg1	Model					962:966	Laboratoire de Météorologie Dynamique General Circulation Model	904:966	Laboratoire de Météorologie Dynamique General Circulation Model	904:966	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	0	20	theme	tree-ring	36:44	arg1	cellulose					46:54	tree-ring cellulose	36:54	tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau	36:124	Air mass origin signals in δ 18O of tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau.
28032196	3	21	theme	novel	600:604	arg1	approach					606:613	This novel approach	595:613	This novel approach	595:613	This novel approach has been tested for a transect at the southeastern Tibetan plateau (TP), where air masses with different isotopic composition overlap.
28032196	1	22	theme	profound	129:136	arg1	precondition					203:214	a precondition	201:214	a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives	201:298	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	1	22	theme	profound	129:136	arg1	consideration					138:150	A profound consideration	127:150	A profound consideration of stable oxygen isotope source water origins	127:196	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	4	23	theme	Model	962:966	arg1	data					978:981	the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data	884:981	the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data	884:981	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	4	23	theme	Model	962:966	arg1	LMDZiso					969:975	LMDZiso	969:975	LMDZiso	969:975	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	8	24	theme	hydroclimate	1423:1434	arg1	history					1436:1442	the local hydroclimate history	1413:1442	the local hydroclimate history inferred from [Formula: see text] archives	1413:1485	That implies that the local hydroclimate history inferred from [Formula: see text] archives is better recorded at semi-humid sites.
28032196	2	25	from	influence	315:323	arg1	chronologies					376:387	widely used δ 18O tree-ring chronologies	348:387	widely used δ 18O tree-ring chronologies	348:387	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	4	26	theme	Laboratoire	904:914	arg1	Model					962:966	Laboratoire de Météorologie Dynamique General Circulation Model	904:966	Laboratoire de Météorologie Dynamique General Circulation Model	904:966	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	0	27	theme	back-trajectory	68:82	arg1	modeling					84:91	back-trajectory modeling	68:91	back-trajectory modeling at the monsoonal Tibetan plateau	68:124	Air mass origin signals in δ 18O of tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau.
28032196	5	28	theme	distinct	1060:1067	arg1	effect					1076:1081	a distinct amount effect	1058:1081	a distinct amount effect	1058:1081	Particularly the southernmost study sites are influenced by a distinct amount effect.
28032196	8	29	theme	[Formula	1458:1465	arg1	archives					1478:1485	[Formula: see text] archives	1458:1485	[Formula: see text] archives	1458:1485	That implies that the local hydroclimate history inferred from [Formula: see text] archives is better recorded at semi-humid sites.
28032196	4	30	theme	Detailed	750:757	arg1	examinations					759:770	Detailed examinations	750:770	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text])	750:862	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	3	31	with	masses	698:703	arg1	composition					729:739	different isotopic composition	710:739	different isotopic composition	710:739	This novel approach has been tested for a transect at the southeastern Tibetan plateau (TP), where air masses with different isotopic composition overlap.
28032196	2	32	theme	resolved	445:452	arg1	chronologies					502:513	six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies	432:513	six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies	432:513	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	2	33	theme	tree-ring	366:374	arg1	chronologies					376:387	widely used δ 18O tree-ring chronologies	348:387	widely used δ 18O tree-ring chronologies	348:387	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	4	34	dep	values	835:840	arg1	[Formula					843:850	[Formula	843:850	[Formula: see text]	843:861	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	1	35	theme	δ	285:285	arg1	archives					291:298	terrestrial δ 18O archives	273:298	terrestrial δ 18O archives	273:298	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	1	36	theme	archives	291:298	arg1	interpretation					255:268	an unambiguous palaeoenvironmental interpretation	220:268	an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives	220:298	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	2	37	theme	cellulose	470:478	arg1	chronologies					502:513	six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies	432:513	six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies	432:513	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	2	38	theme	18O	456:458	arg1	chronologies					502:513	six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies	432:513	six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies	432:513	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	4	39	theme	precipitation	815:827	arg1	values					835:840	monthly precipitation δ 18O values	807:840	monthly precipitation δ 18O values ([Formula: see text])	807:862	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	2	40	theme	origins	337:343	arg1	influence					315:323	the influence	311:323	the influence of air mass origins on widely used δ 18O tree-ring chronologies	311:387	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	4	41	dep	[Formula	843:850	arg1	see					853:855	see	853:855	see text	853:860	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	6	42	theme	air	1090:1092	arg1	[Formula					1109:1116	air package origin [Formula	1090:1116	air package origin [Formula: see text] relations	1090:1137	Here, air package origin [Formula: see text] relations are generally weaker in contrast to our northern located study sites.
28032196	8	43	theme	semi-humid	1509:1518	arg1	sites					1520:1524	semi-humid sites	1509:1524	semi-humid sites	1509:1524	That implies that the local hydroclimate history inferred from [Formula: see text] archives is better recorded at semi-humid sites.
28032196	3	44	theme	southeastern	653:664	arg1	TP					683:684	TP	683:684	TP	683:684	This novel approach has been tested for a transect at the southeastern Tibetan plateau (TP), where air masses with different isotopic composition overlap.
28032196	3	44	theme	southeastern	653:664	arg1	plateau					674:680	the southeastern Tibetan plateau	649:680	the southeastern Tibetan plateau (TP)	649:685	This novel approach has been tested for a transect at the southeastern Tibetan plateau (TP), where air masses with different isotopic composition overlap.
28032196	2	45	theme	air	328:330	arg1	origins					337:343	air mass origins	328:343	air mass origins	328:343	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	0	46	from	plateau	118:124	arg1	modeling					84:91	back-trajectory modeling	68:91	back-trajectory modeling at the monsoonal Tibetan plateau	68:124	Air mass origin signals in δ 18O of tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau.
28032196	4	47	theme	precipitation	781:793	arg1	amounts					795:801	daily precipitation amounts	775:801	daily precipitation amounts	775:801	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	2	48	theme	δ	360:360	arg1	chronologies					376:387	widely used δ 18O tree-ring chronologies	348:387	widely used δ 18O tree-ring chronologies	348:387	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	1	49	theme	palaeoenvironmental	235:253	arg1	interpretation					255:268	an unambiguous palaeoenvironmental interpretation	220:268	an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives	220:298	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	1	50	theme	unambiguous	223:233	arg1	interpretation					255:268	an unambiguous palaeoenvironmental interpretation	220:268	an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives	220:298	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	2	51	theme	backward	565:572	arg1	modeling					585:592	backward trajectory modeling	565:592	backward trajectory modeling	565:592	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	3	52	theme	air	694:696	arg1	masses					698:703	air masses	694:703	air masses with different isotopic composition	694:739	This novel approach has been tested for a transect at the southeastern Tibetan plateau (TP), where air masses with different isotopic composition overlap.
28032196	1	53	theme	stable	155:160	arg1	origins					190:196	stable oxygen isotope source water origins	155:196	stable oxygen isotope source water origins	155:196	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	7	54	theme	semi-humid	1377:1386	arg1	sites					1388:1392	semi-humid sites	1377:1392	semi-humid sites	1377:1392	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	4	55	theme	δ	829:829	arg1	values					835:840	monthly precipitation δ 18O values	807:840	monthly precipitation δ 18O values ([Formula: see text])	807:862	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	7	56	theme	isotope	1359:1365	arg1	values					1367:1372	tree-ring isotope values	1349:1372	tree-ring isotope values at semi-humid sites	1349:1392	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	1	57	theme	isotope	169:175	arg1	origins					190:196	stable oxygen isotope source water origins	155:196	stable oxygen isotope source water origins	155:196	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	7	58	from	sites	1388:1392	arg1	values					1367:1372	tree-ring isotope values	1349:1372	tree-ring isotope values at semi-humid sites	1349:1392	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	4	59	theme	values	835:840	arg1	examinations					759:770	Detailed examinations	750:770	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text])	750:862	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	3	60	theme	isotopic	720:727	arg1	composition					729:739	different isotopic composition	710:739	different isotopic composition	710:739	This novel approach has been tested for a transect at the southeastern Tibetan plateau (TP), where air masses with different isotopic composition overlap.
28032196	0	61	theme	Tibetan	110:116	arg1	plateau					118:124	the monsoonal Tibetan plateau	96:124	the monsoonal Tibetan plateau	96:124	Air mass origin signals in δ 18O of tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau.
28032196	1	62	theme	water	184:188	arg1	origins					190:196	stable oxygen isotope source water origins	155:196	stable oxygen isotope source water origins	155:196	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	2	63	theme	annual	524:529	arg1	origins					543:549	mean annual air package origins	519:549	mean annual air package origins	519:549	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	7	64	theme	mass	1332:1335	arg1	origin					1337:1342	air mass origin	1328:1342	air mass origin than tree-ring isotope values at semi-humid sites	1328:1392	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	7	65	theme	isotope	1233:1239	arg1	signatures					1241:1250	tree-ring isotope signatures	1223:1250	tree-ring isotope signatures at dry sites with less rain days per year	1223:1292	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	4	66	dep	Laboratoire	904:914	arg1	de					916:917	Laboratoire de Météorologie Dynamique	904:940	Laboratoire de Météorologie Dynamique General Circulation Model	904:966	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	4	66	dep	Laboratoire	904:914	arg1	Dynamique					932:940	Dynamique	932:940	Dynamique	932:940	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	4	67	theme	ERA	888:890	arg1	Interim					892:898	the ERA Interim	884:898	the ERA Interim	884:898	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	7	68	with	signatures	1241:1250	arg1	days					1280:1283	less rain days	1270:1283	less rain days per year	1270:1292	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	5	69	theme	southernmost	1015:1026	arg1	sites					1034:1038	Particularly the southernmost study sites	998:1038	Particularly the southernmost study sites	998:1038	Particularly the southernmost study sites are influenced by a distinct amount effect.
28032196	7	70	theme	air	1328:1330	arg1	origin					1337:1342	air mass origin	1328:1342	air mass origin than tree-ring isotope values at semi-humid sites	1328:1392	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	2	71	theme	package	535:541	arg1	origins					543:549	mean annual air package origins	519:549	mean annual air package origins	519:549	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	8	72	theme	local	1417:1421	arg1	history					1436:1442	the local hydroclimate history	1413:1442	the local hydroclimate history inferred from [Formula: see text] archives	1413:1485	That implies that the local hydroclimate history inferred from [Formula: see text] archives is better recorded at semi-humid sites.
28032196	0	73	theme	cellulose	46:54	arg1	18O					29:31	δ 18O	27:31	δ 18O of tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau	27:124	Air mass origin signals in δ 18O of tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau.
28032196	4	74	theme	Circulation	950:960	arg1	Model					962:966	Laboratoire de Météorologie Dynamique General Circulation Model	904:966	Laboratoire de Météorologie Dynamique General Circulation Model	904:966	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	2	75	dep	cellulose	470:478	arg1	see					491:493	see	491:493	see text	491:498	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	6	76	theme	origin	1102:1107	arg1	[Formula					1109:1116	air package origin [Formula	1090:1116	air package origin [Formula: see text] relations	1090:1137	Here, air package origin [Formula: see text] relations are generally weaker in contrast to our northern located study sites.
28032196	5	77	theme	amount	1069:1074	arg1	effect					1076:1081	a distinct amount effect	1058:1081	a distinct amount effect	1058:1081	Particularly the southernmost study sites are influenced by a distinct amount effect.
28032196	8	78	dep	[Formula	1458:1465	arg1	see					1468:1470	see	1468:1470	see text	1468:1475	That implies that the local hydroclimate history inferred from [Formula: see text] archives is better recorded at semi-humid sites.
28032196	7	79	theme	rain	1275:1278	arg1	days					1280:1283	less rain days	1270:1283	less rain days per year	1270:1292	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	0	80	theme	mass	4:7	arg1	origin					9:14	Air mass origin	0:14	Air mass origin	0:14	Air mass origin signals in δ 18O of tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau.
28032196	2	81	theme	mean	519:522	arg1	origins					543:549	mean annual air package origins	519:549	mean annual air package origins	519:549	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	2	82	theme	18O	362:364	arg1	chronologies					376:387	widely used δ 18O tree-ring chronologies	348:387	widely used δ 18O tree-ring chronologies	348:387	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	1	83	theme	18O	287:289	arg1	archives					291:298	terrestrial δ 18O archives	273:298	terrestrial δ 18O archives	273:298	A profound consideration of stable oxygen isotope source water origins is a precondition for an unambiguous palaeoenvironmental interpretation of terrestrial δ 18O archives.
28032196	7	84	theme	dry	1255:1257	arg1	sites					1259:1263	dry sites	1255:1263	dry sites	1255:1263	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	2	85	theme	correlation	403:413	arg1	analyses					415:422	correlation analyses	403:422	correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling	403:592	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	0	86	theme	δ	27:27	arg1	18O					29:31	δ 18O	27:31	δ 18O of tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau	27:124	Air mass origin signals in δ 18O of tree-ring cellulose revealed by back-trajectory modeling at the monsoonal Tibetan plateau.
28032196	2	87	theme	tree-ring	460:468	arg1	cellulose					470:478	tree-ring cellulose	460:478	six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies	432:513	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	4	88	theme	amounts	795:801	arg1	examinations					759:770	Detailed examinations	750:770	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text])	750:862	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	6	89	theme	package	1094:1100	arg1	[Formula					1109:1116	air package origin [Formula	1090:1116	air package origin [Formula: see text] relations	1090:1137	Here, air package origin [Formula: see text] relations are generally weaker in contrast to our northern located study sites.
28032196	2	90	theme	δ	454:454	arg1	chronologies					502:513	six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies	432:513	six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies	432:513	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	4	91	theme	monthly	807:813	arg1	values					835:840	monthly precipitation δ 18O values	807:840	monthly precipitation δ 18O values ([Formula: see text])	807:862	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	2	92	theme	mass	332:335	arg1	origins					337:343	air mass origins	328:343	air mass origins	328:343	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	2	93	dep	see	491:493	arg1	[Formula					481:488	[Formula	481:488	[Formula	481:488	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	7	94	from	sites	1259:1263	arg1	signatures					1241:1250	tree-ring isotope signatures	1223:1250	tree-ring isotope signatures at dry sites with less rain days per year	1223:1292	We found that tree-ring isotope signatures at dry sites with less rain days per year tend to be influenced stronger by air mass origin than tree-ring isotope values at semi-humid sites.
28032196	4	95	theme	daily	775:779	arg1	amounts					795:801	daily precipitation amounts	775:801	daily precipitation amounts	775:801	Detailed examinations of daily precipitation amounts and monthly precipitation δ 18O values ([Formula: see text]) were conducted with the ERA Interim and Laboratoire de Météorologie Dynamique General Circulation Model (LMDZiso) data, respectively.
28032196	2	96	theme	used	355:358	arg1	chronologies					376:387	widely used δ 18O tree-ring chronologies	348:387	widely used δ 18O tree-ring chronologies	348:387	To stress the influence of air mass origins on widely used δ 18O tree-ring chronologies, we conducted correlation analyses between six annually resolved δ 18O tree-ring cellulose ([Formula: see text]) chronologies and mean annual air package origins obtained from backward trajectory modeling.
28032196	3	97	dep	a	635:635	arg1	transect					637:644	transect	637:644	transect at the southeastern Tibetan plateau (TP), where air masses with different isotopic composition overlap	637:747	This novel approach has been tested for a transect at the southeastern Tibetan plateau (TP), where air masses with different isotopic composition overlap.
25563959	1	0	theme	viscose/wool	351:362	arg1	blends					364:369	viscose/wool blends	351:369	viscose/wool blends	351:369	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	2	1	theme	microwave	642:650	arg1	fixation					652:659	the pad-dry microwave fixation	630:659	the pad-dry microwave fixation technique	630:669	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	7	2	dep	using	1281:1285	arg1	possible					1272:1279	possible	1272:1279	possible	1272:1279	Practical applications for multifunctionlization of cellulose/wool blended fabrics are possible using these sorts of proper finishing formulations and unique finishing application method.
25563959	1	3	theme	one-bath	163:170	arg1	procedure					193:201	a one-bath functional finishing procedure	161:201	a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations	161:423	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	2	4	theme	pad-dry	634:640	arg1	fixation					652:659	the pad-dry microwave fixation	630:659	the pad-dry microwave fixation technique	630:669	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	1	5	theme	functional	172:181	arg1	procedure					193:201	a one-bath functional finishing procedure	161:201	a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations	161:423	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	7	6	theme	proper	1302:1307	arg1	formulations					1319:1330	proper finishing formulations	1302:1330	proper finishing formulations	1302:1330	Practical applications for multifunctionlization of cellulose/wool blended fabrics are possible using these sorts of proper finishing formulations and unique finishing application method.
25563959	7	7	theme	Practical	1185:1193	arg1	applications					1195:1206	Practical applications	1185:1206	Practical applications for multifunctionlization of cellulose/wool blended fabrics	1185:1266	Practical applications for multifunctionlization of cellulose/wool blended fabrics are possible using these sorts of proper finishing formulations and unique finishing application method.
25563959	7	8	theme	finishing	1309:1317	arg1	formulations					1319:1330	proper finishing formulations	1302:1330	proper finishing formulations	1302:1330	Practical applications for multifunctionlization of cellulose/wool blended fabrics are possible using these sorts of proper finishing formulations and unique finishing application method.
25563959	1	9	theme	diverse	377:383	arg1	combinations					395:406	diverse finishing combinations	377:406	diverse finishing combinations	377:406	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	7	10	dep	these	1287:1291	arg1	sorts					1293:1297	sorts	1293:1297	sorts	1293:1297	Practical applications for multifunctionlization of cellulose/wool blended fabrics are possible using these sorts of proper finishing formulations and unique finishing application method.
25563959	7	11	theme	application	1353:1363	arg1	method					1365:1370	unique finishing application method	1336:1370	unique finishing application method	1336:1370	Practical applications for multifunctionlization of cellulose/wool blended fabrics are possible using these sorts of proper finishing formulations and unique finishing application method.
25563959	1	12	theme	finishing	385:393	arg1	combinations					395:406	diverse finishing combinations	377:406	diverse finishing combinations	377:406	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	4	13	theme	high	902:905	arg1	level					907:911	high level	902:911	high level of functionalities	902:930	The finished fabrics still retained high level of functionalities even after 15 consecutive laundering.
25563959	5	14	theme	electron	1054:1061	arg1	SEM					1075:1077	SEM	1075:1077	SEM	1075:1077	Surface morphology and composition of selected samples were investigated using scan electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis.
25563959	5	14	theme	electron	1054:1061	arg1	microscope					1063:1072	electron microscope	1054:1072	scan electron microscope (SEM)	1049:1078	Surface morphology and composition of selected samples were investigated using scan electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis.
25563959	1	15	theme	finishing	183:191	arg1	procedure					193:201	a one-bath functional finishing procedure	161:201	a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations	161:423	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	0	16	theme	multifunctional	50:64	arg1	cotton/wool					66:76	durable multifunctional cotton/wool	42:76	durable multifunctional cotton/wool	42:76	New finishing possibilities for producing durable multifunctional cotton/wool and viscose/wool blended fabrics.
25563959	7	17	theme	blended	1252:1258	arg1	fabrics					1260:1266	cellulose/wool blended fabrics	1237:1266	cellulose/wool blended fabrics	1237:1266	Practical applications for multifunctionlization of cellulose/wool blended fabrics are possible using these sorts of proper finishing formulations and unique finishing application method.
25563959	1	18	theme	procedure	193:201	arg1	development					146:156	the development	142:156	the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations	142:423	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	0	19	theme	finishing	4:12	arg1	possibilities					14:26	New finishing possibilities	0:26	New finishing possibilities for producing durable multifunctional cotton/wool and viscose/wool	0:93	New finishing possibilities for producing durable multifunctional cotton/wool and viscose/wool blended fabrics.
25563959	5	20	theme	selected	1008:1015	arg1	samples					1017:1023	selected samples	1008:1023	selected samples	1008:1023	Surface morphology and composition of selected samples were investigated using scan electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis.
25563959	3	21	theme	finished	774:781	arg1	substrate					783:791	the finished substrate	770:791	the finished substrate	770:791	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
25563959	0	22	theme	New	0:2	arg1	possibilities					14:26	New finishing possibilities	0:26	New finishing possibilities for producing durable multifunctional cotton/wool and viscose/wool	0:93	New finishing possibilities for producing durable multifunctional cotton/wool and viscose/wool blended fabrics.
25563959	1	23	theme	ultra	303:307	arg1	protection					321:330	ultra violet (UV) protection	303:330	ultra violet (UV) protection	303:330	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	2	24	theme	fixation	652:659	arg1	technique					661:669	the pad-dry microwave fixation technique	630:669	the pad-dry microwave fixation technique	630:669	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	5	25	theme	samples	1017:1023	arg1	composition					993:1003	composition	993:1003	composition	993:1003	Surface morphology and composition of selected samples were investigated using scan electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis.
25563959	5	25	theme	samples	1017:1023	arg1	morphology					978:987	Surface morphology	970:987	Surface morphology	970:987	Surface morphology and composition of selected samples were investigated using scan electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis.
25563959	4	26	theme	functionalities	916:930	arg1	level					907:911	high level	902:911	high level of functionalities	902:930	The finished fabrics still retained high level of functionalities even after 15 consecutive laundering.
25563959	2	27	theme	reactant	465:472	arg1	resin					474:478	reactant resin	465:478	reactant resin	465:478	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	1	28	theme	violet	309:314	arg1	protection					321:330	ultra violet (UV) protection	303:330	ultra violet (UV) protection	303:330	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	1	29	theme	research	117:124	arg1	work					126:129	This research work	112:129	This research work	112:129	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	5	30	theme	energy-dispersive	1084:1100	arg1	analysis					1127:1134	energy-dispersive X-ray spectroscopy (EDX) analysis	1084:1134	energy-dispersive X-ray spectroscopy (EDX) analysis	1084:1134	Surface morphology and composition of selected samples were investigated using scan electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis.
25563959	1	31	theme	durable	217:223	arg1	care					265:268	easy care	260:268	easy care	260:268	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	1	31	theme	durable	217:223	arg1	protection					321:330	ultra violet (UV) protection	303:330	ultra violet (UV) protection	303:330	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	1	31	theme	durable	217:223	arg1	antibacterial					282:294	antibacterial	282:294	antibacterial	282:294	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	1	31	theme	durable	217:223	arg1	properties					241:250	durable multifunctional properties	217:250	durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection	217:330	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	1	31	theme	durable	217:223	arg1	soft-hand					271:279	soft-hand	271:279	soft-hand	271:279	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	3	32	theme	substrate	783:791	arg1	concentration					815:827	concentration	815:827	concentration	815:827	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
25563959	3	32	theme	substrate	783:791	arg1	nature					804:809	nature	804:809	nature	804:809	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
25563959	3	32	theme	substrate	783:791	arg1	type					762:765	type	762:765	type of the finished substrate as well as nature and concentration of finishing formulation components	762:863	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
25563959	2	33	theme	chloride/citric	585:599	arg1	acid					601:604	magnesium chloride/citric acid	575:604	magnesium chloride/citric acid	575:604	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	2	33	theme	chloride/citric	585:599	arg1	catalyst					617:624	a mixed catalyst	609:624	a mixed catalyst	609:624	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	5	34	theme	Surface	970:976	arg1	morphology					978:987	Surface morphology	970:987	Surface morphology	970:987	Surface morphology and composition of selected samples were investigated using scan electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis.
25563959	1	35	theme	multifunctional	225:239	arg1	care					265:268	easy care	260:268	easy care	260:268	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	1	35	theme	multifunctional	225:239	arg1	protection					321:330	ultra violet (UV) protection	303:330	ultra violet (UV) protection	303:330	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	1	35	theme	multifunctional	225:239	arg1	antibacterial					282:294	antibacterial	282:294	antibacterial	282:294	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	1	35	theme	multifunctional	225:239	arg1	properties					241:250	durable multifunctional properties	217:250	durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection	217:330	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	1	35	theme	multifunctional	225:239	arg1	soft-hand					271:279	soft-hand	271:279	soft-hand	271:279	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	0	36	theme	durable	42:48	arg1	cotton/wool					66:76	durable multifunctional cotton/wool	42:76	durable multifunctional cotton/wool	42:76	New finishing possibilities for producing durable multifunctional cotton/wool and viscose/wool blended fabrics.
25563959	1	37	theme	UV	317:318	arg1	protection					321:330	ultra violet (UV) protection	303:330	ultra violet (UV) protection	303:330	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	2	38	theme	magnesium	575:583	arg1	acid					601:604	magnesium chloride/citric acid	575:604	magnesium chloride/citric acid	575:604	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	2	38	theme	magnesium	575:583	arg1	catalyst					617:624	a mixed catalyst	609:624	a mixed catalyst	609:624	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	4	39	theme	finished	870:877	arg1	fabrics					879:885	The finished fabrics	866:885	The finished fabrics	866:885	The finished fabrics still retained high level of functionalities even after 15 consecutive laundering.
25563959	2	40	theme	finishing	440:448	arg1	colorant					546:553	pigment colorant	538:553	pigment colorant	538:553	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	2	40	theme	finishing	440:448	arg1	resin					474:478	reactant resin	465:478	reactant resin	465:478	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	2	40	theme	finishing	440:448	arg1	triclosan					523:531	triclosan	523:531	triclosan	523:531	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	2	40	theme	finishing	440:448	arg1	agents					450:455	finishing agents	440:455	finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant	440:553	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	2	40	theme	finishing	440:448	arg1	softeners					489:497	silicon softeners	481:497	silicon softeners	481:497	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	2	40	theme	finishing	440:448	arg1	4-hydroxybenzophenone					500:520	4-hydroxybenzophenone	500:520	4-hydroxybenzophenone	500:520	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	6	41	theme	interactions	1149:1160	arg1	mode					1141:1144	The mode	1137:1144	The mode of interactions	1137:1160	The mode of interactions was also investigated.
25563959	7	42	theme	fabrics	1260:1266	arg1	multifunctionlization					1212:1232	multifunctionlization	1212:1232	multifunctionlization of cellulose/wool blended fabrics	1212:1266	Practical applications for multifunctionlization of cellulose/wool blended fabrics are possible using these sorts of proper finishing formulations and unique finishing application method.
25563959	5	43	theme	X-ray	1102:1106	arg1	EDX					1122:1124	EDX	1122:1124	EDX	1122:1124	Surface morphology and composition of selected samples were investigated using scan electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis.
25563959	5	43	theme	X-ray	1102:1106	arg1	spectroscopy					1108:1119	X-ray spectroscopy	1102:1119	energy-dispersive X-ray spectroscopy (EDX) analysis	1084:1134	Surface morphology and composition of selected samples were investigated using scan electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis.
25563959	2	44	theme	pigment	538:544	arg1	colorant					546:553	pigment colorant	538:553	pigment colorant	538:553	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	5	45	theme	spectroscopy	1108:1119	arg1	analysis					1127:1134	energy-dispersive X-ray spectroscopy (EDX) analysis	1084:1134	energy-dispersive X-ray spectroscopy (EDX) analysis	1084:1134	Surface morphology and composition of selected samples were investigated using scan electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis.
25563959	2	46	theme	silicon	481:487	arg1	softeners					489:497	silicon softeners	481:497	silicon softeners	481:497	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	2	47	theme	mixed	611:615	arg1	acid					601:604	magnesium chloride/citric acid	575:604	magnesium chloride/citric acid	575:604	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	2	47	theme	mixed	611:615	arg1	catalyst					617:624	a mixed catalyst	609:624	a mixed catalyst	609:624	In this study finishing agents such as reactant resin, silicon softeners, 4-hydroxybenzophenone, triclosan, and pigment colorant were selected using magnesium chloride/citric acid as a mixed catalyst and the pad-dry microwave fixation technique.
25563959	3	48	theme	imparted	715:722	arg1	properties					735:744	the imparted functional properties	711:744	the imparted functional properties	711:744	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
25563959	5	49	dep	microscope	1063:1072	arg1	scan					1049:1052	scan	1049:1052	scan	1049:1052	Surface morphology and composition of selected samples were investigated using scan electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis.
25563959	3	50	from	enhancement	696:706	arg1	properties					735:744	the imparted functional properties	711:744	the imparted functional properties	711:744	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
25563959	3	51	theme	functional	724:733	arg1	properties					735:744	the imparted functional properties	711:744	the imparted functional properties	711:744	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
25563959	7	52	theme	cellulose/wool	1237:1250	arg1	fabrics					1260:1266	cellulose/wool blended fabrics	1237:1266	cellulose/wool blended fabrics	1237:1266	Practical applications for multifunctionlization of cellulose/wool blended fabrics are possible using these sorts of proper finishing formulations and unique finishing application method.
25563959	3	53	theme	components	854:863	arg1	concentration					815:827	concentration	815:827	concentration	815:827	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
25563959	3	53	theme	components	854:863	arg1	nature					804:809	nature	804:809	nature	804:809	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
25563959	3	53	theme	components	854:863	arg1	type					762:765	type	762:765	type of the finished substrate as well as nature and concentration of finishing formulation components	762:863	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
25563959	1	54	theme	easy	260:263	arg1	care					265:268	easy care	260:268	easy care	260:268	This research work focuses on the development of a one-bath functional finishing procedure for imparting durable multifunctional properties such as easy care, soft-hand, antibacterial and/or ultra violet (UV) protection to cotton/wool and viscose/wool blends using diverse finishing combinations and formulations.
25563959	7	55	theme	unique	1336:1341	arg1	method					1365:1370	unique finishing application method	1336:1370	unique finishing application method	1336:1370	Practical applications for multifunctionlization of cellulose/wool blended fabrics are possible using these sorts of proper finishing formulations and unique finishing application method.
25563959	4	56	theme	consecutive	946:956	arg1	laundering					958:967	15 consecutive laundering	943:967	15 consecutive laundering	943:967	The finished fabrics still retained high level of functionalities even after 15 consecutive laundering.
25563959	3	57	theme	finishing	832:840	arg1	components					854:863	finishing formulation components	832:863	finishing formulation components	832:863	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
25563959	7	58	theme	finishing	1343:1351	arg1	method					1365:1370	unique finishing application method	1336:1370	unique finishing application method	1336:1370	Practical applications for multifunctionlization of cellulose/wool blended fabrics are possible using these sorts of proper finishing formulations and unique finishing application method.
25563959	3	59	theme	formulation	842:852	arg1	components					854:863	finishing formulation components	832:863	finishing formulation components	832:863	The results reveal that enhancement in the imparted functional properties are governed by type of the finished substrate as well as nature and concentration of finishing formulation components.
29169107	0	0	theme	terahertz	75:83	arg1	analyses					105:112	terahertz time-domain spectra analyses	75:112	terahertz time-domain spectra analyses	75:112	Mechanistic insights into alginate fouling caused by calcium ions based on terahertz time-domain spectra analyses and DFT calculations.
29169107	3	1	theme	photoelectric	722:734	arg1	XPS					750:752	XPS	750:752	XPS	750:752	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	1	theme	photoelectric	722:734	arg1	Fourier					756:762	Fourier	756:762	Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA)	756:838	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	1	theme	photoelectric	722:734	arg1	spectroscopy					736:747	X-ray photoelectric spectroscopy	716:747	X-ray photoelectric spectroscopy (XPS)	716:753	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	6	2	theme	filtration	1556:1565	arg1	behaviors					1567:1575	the filtration behaviors	1552:1575	the filtration behaviors of alginate solution caused by calcium addition	1552:1623	According to these results, "chemical potential gap" depicted by Flory-Huggins theory was suggested to be responsible for the filtration behaviors of alginate solution caused by calcium addition.
29169107	2	3	with	SFR	565:567	arg1	addition					631:638	1.0 mM calcium addition	616:638	1.0 mM calcium addition	616:638	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	1	4	theme	time-domain	270:280	arg1	spectroscopy					282:293	Terahertz time-domain spectroscopy	260:293	Terahertz time-domain spectroscopy (THz-TDS)	260:303	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	1	4	theme	time-domain	270:280	arg1	THz-TDS					296:302	THz-TDS	296:302	THz-TDS	296:302	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	3	5	dep	Fourier	756:762	arg1	transform					764:772	transform	764:772	transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA)	764:838	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	5	6	theme	chains	1166:1171	arg1	binding					1146:1152	initial binding	1138:1152	initial binding of alginate chains induced by calcium ions	1138:1195	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	2	7	theme	solution	602:609	arg1	2.61 × 1015 m kg-1					570:587	2.61 × 1015 m kg-1	570:587	2.61 × 1015 m kg-1	570:587	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	2	7	theme	solution	602:609	arg1	SFR					565:567	SFR	565:567	SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition	565:638	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	1	8	theme	filtration	170:179	arg1	behaviors					181:189	the filtration behaviors	166:189	the filtration behaviors of alginate solution caused by calcium addition	166:237	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	3	9	theme	groups	882:887	arg1	composition					856:866	the composition	852:866	the composition of functional groups, elements and thermal stability of alginate	852:931	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	0	10	theme	spectra	97:103	arg1	analyses					105:112	terahertz time-domain spectra analyses	75:112	terahertz time-domain spectra analyses	75:112	Mechanistic insights into alginate fouling caused by calcium ions based on terahertz time-domain spectra analyses and DFT calculations.
29169107	2	11	theme	alginate	593:600	arg1	solution					602:609	alginate solution	593:609	alginate solution	593:609	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	4	12	from	variation	1059:1067	arg1	solution					1092:1099	solution	1092:1099	solution	1092:1099	Howbeit, THz-TDS spectra showed that calcium addition caused structural variation of alginate polymer in solution.
29169107	6	13	theme	potential	1468:1476	arg1	"					1481:1481	"chemical potential gap"	1458:1481	"chemical potential gap" depicted by Flory-Huggins theory	1458:1514	According to these results, "chemical potential gap" depicted by Flory-Huggins theory was suggested to be responsible for the filtration behaviors of alginate solution caused by calcium addition.
29169107	0	14	theme	time-domain	85:95	arg1	analyses					105:112	terahertz time-domain spectra analyses	75:112	terahertz time-domain spectra analyses	75:112	Mechanistic insights into alginate fouling caused by calcium ions based on terahertz time-domain spectra analyses and DFT calculations.
29169107	5	15	dep	structure	1363:1371	arg1	cross					1374:1378	cross	1374:1378	cross	1374:1378	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	5	16	theme	tetrahedron	1351:1361	arg1	structure					1363:1371	a tetrahedron structure	1349:1371	a tetrahedron structure (cross to each other) other than parallel to each other	1349:1427	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	2	17	theme	calcium	527:533	arg1	0-1.0 mM					550:557	0-1.0 mM	550:557	0-1.0 mM	550:557	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	2	17	theme	calcium	527:533	arg1	concentration					535:547	calcium concentration	527:547	calcium concentration (0-1.0 mM)	527:558	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	6	18	theme	alginate	1580:1587	arg1	solution					1589:1596	alginate solution	1580:1596	alginate solution caused by calcium addition	1580:1623	According to these results, "chemical potential gap" depicted by Flory-Huggins theory was suggested to be responsible for the filtration behaviors of alginate solution caused by calcium addition.
29169107	4	19	theme	alginate	1072:1079	arg1	polymer					1081:1087	alginate polymer	1072:1087	alginate polymer	1072:1087	Howbeit, THz-TDS spectra showed that calcium addition caused structural variation of alginate polymer in solution.
29169107	3	20	theme	alginate	924:931	arg1	elements					890:897	elements	890:897	elements	890:897	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	20	theme	alginate	924:931	arg1	stability					911:919	thermal stability	903:919	thermal stability	903:919	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	20	theme	alginate	924:931	arg1	groups					882:887	functional groups	871:887	functional groups	871:887	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	5	21	theme	initial	1138:1144	arg1	binding					1146:1152	initial binding	1138:1152	initial binding of alginate chains induced by calcium ions	1138:1195	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	6	22	theme	solution	1589:1596	arg1	behaviors					1567:1575	the filtration behaviors	1552:1575	the filtration behaviors of alginate solution caused by calcium addition	1552:1623	According to these results, "chemical potential gap" depicted by Flory-Huggins theory was suggested to be responsible for the filtration behaviors of alginate solution caused by calcium addition.
29169107	3	23	theme	thermal	903:909	arg1	stability					911:919	thermal stability	903:919	thermal stability	903:919	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	23	theme	thermal	903:909	arg1	groups					882:887	functional groups	871:887	functional groups	871:887	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	4	24	theme	structural	1048:1057	arg1	variation					1059:1067	structural variation	1048:1067	structural variation of alginate polymer in solution	1048:1099	Howbeit, THz-TDS spectra showed that calcium addition caused structural variation of alginate polymer in solution.
29169107	2	25	theme	calcium	623:629	arg1	addition					631:638	1.0 mM calcium addition	616:638	1.0 mM calcium addition	616:638	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	6	26	theme	chemical	1459:1466	arg1	"					1481:1481	"chemical potential gap"	1458:1481	"chemical potential gap" depicted by Flory-Huggins theory	1458:1514	According to these results, "chemical potential gap" depicted by Flory-Huggins theory was suggested to be responsible for the filtration behaviors of alginate solution caused by calcium addition.
29169107	1	27	theme	alginate	194:201	arg1	solution					203:210	alginate solution	194:210	alginate solution caused by calcium addition	194:237	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	0	28	theme	Mechanistic	0:10	arg1	insights					12:19	Mechanistic insights	0:19	Mechanistic insights into alginate fouling	0:41	Mechanistic insights into alginate fouling caused by calcium ions based on terahertz time-domain spectra analyses and DFT calculations.
29169107	7	29	theme	mechanistic	1650:1660	arg1	insights					1662:1669	the mechanistic insights	1646:1669	the mechanistic insights into membrane fouling	1646:1691	This study provided the mechanistic insights into membrane fouling.
29169107	2	30	theme	1.0 mM	616:621	arg1	addition					631:638	1.0 mM calcium addition	616:638	1.0 mM calcium addition	616:638	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	2	31	theme	sludge	676:681	arg1	suspension					683:692	sludge suspension	676:692	sludge suspension	676:692	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	1	32	theme	solution	203:210	arg1	behaviors					181:189	the filtration behaviors	166:189	the filtration behaviors of alginate solution caused by calcium addition	166:237	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	0	33	theme	alginate	26:33	arg1	fouling					35:41	alginate fouling	26:41	alginate fouling	26:41	Mechanistic insights into alginate fouling caused by calcium ions based on terahertz time-domain spectra analyses and DFT calculations.
29169107	3	34	theme	infrared	774:781	arg1	FTIR					797:800	FTIR	797:800	FTIR	797:800	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	34	theme	infrared	774:781	arg1	spectroscopy					783:794	infrared spectroscopy	774:794	infrared spectroscopy (FTIR)	774:801	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	6	35	theme	gap	1478:1480	arg1	"					1481:1481	"chemical potential gap"	1458:1481	"chemical potential gap" depicted by Flory-Huggins theory	1458:1514	According to these results, "chemical potential gap" depicted by Flory-Huggins theory was suggested to be responsible for the filtration behaviors of alginate solution caused by calcium addition.
29169107	5	36	theme	alginate	1157:1164	arg1	chains					1166:1171	alginate chains	1157:1171	alginate chains induced by calcium ions	1157:1195	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	0	37	theme	DFT	118:120	arg1	calculations					122:133	DFT calculations	118:133	DFT calculations	118:133	Mechanistic insights into alginate fouling caused by calcium ions based on terahertz time-domain spectra analyses and DFT calculations.
29169107	2	38	theme	solution	431:438	arg1	SFR					414:416	SFR	414:416	SFR	414:416	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	2	38	theme	solution	431:438	arg1	resistance					402:411	specific filtration resistance	382:411	specific filtration resistance (SFR) of alginate solution (0.75 g L-1)	382:451	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	1	39	theme	functional	317:326	arg1	techniques					341:350	functional theory (DFT) techniques	317:350	functional theory (DFT) techniques	317:350	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	2	40	theme	alginate	422:429	arg1	0.75 g L-1					441:450	0.75 g L-1	441:450	0.75 g L-1	441:450	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	2	40	theme	alginate	422:429	arg1	solution					431:438	alginate solution	422:438	alginate solution (0.75 g L-1)	422:451	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	5	41	dep	other	1239:1243	arg1	than					1245:1248	than	1245:1248	than	1245:1248	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	7	42	theme	membrane	1676:1683	arg1	fouling					1685:1691	membrane fouling	1676:1691	membrane fouling	1676:1691	This study provided the mechanistic insights into membrane fouling.
29169107	1	43	theme	calcium	222:228	arg1	addition					230:237	calcium addition	222:237	calcium addition	222:237	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	1	44	theme	theory	328:333	arg1	techniques					341:350	functional theory (DFT) techniques	317:350	functional theory (DFT) techniques	317:350	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	5	45	theme	calculation	1106:1116	arg1	results					1118:1124	DTF calculation results	1102:1124	DTF calculation results	1102:1124	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	5	46	theme	other	1395:1399	arg1	structure					1363:1371	a tetrahedron structure	1349:1371	a tetrahedron structure (cross to each other) other than parallel to each other	1349:1427	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	0	47	theme	calcium	53:59	arg1	ions					61:64	calcium ions	53:64	calcium ions based on terahertz time-domain spectra analyses and DFT calculations	53:133	Mechanistic insights into alginate fouling caused by calcium ions based on terahertz time-domain spectra analyses and DFT calculations.
29169107	5	48	theme	calcium	1184:1190	arg1	ions					1192:1195	calcium ions	1184:1195	calcium ions	1184:1195	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	2	49	theme	low	514:516	arg1	range					518:522	a relatively low range	501:522	a relatively low range of calcium concentration (0-1.0 mM)	501:558	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	2	50	theme	concentration	535:547	arg1	range					518:522	a relatively low range	501:522	a relatively low range of calcium concentration (0-1.0 mM)	501:558	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	4	51	theme	calcium	1024:1030	arg1	addition					1032:1039	calcium addition	1024:1039	calcium addition	1024:1039	Howbeit, THz-TDS spectra showed that calcium addition caused structural variation of alginate polymer in solution.
29169107	1	52	theme	DFT	336:338	arg1	techniques					341:350	functional theory (DFT) techniques	317:350	functional theory (DFT) techniques	317:350	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	4	53	theme	Howbeit	987:993	arg1	spectra					1004:1010	Howbeit, THz-TDS spectra	987:1010	Howbeit, THz-TDS spectra	987:1010	Howbeit, THz-TDS spectra showed that calcium addition caused structural variation of alginate polymer in solution.
29169107	2	54	theme	Filtration	353:362	arg1	tests					364:368	Filtration tests	353:368	Filtration tests	353:368	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	6	55	theme	Flory-Huggins	1495:1507	arg1	theory					1509:1514	Flory-Huggins theory	1495:1514	Flory-Huggins theory	1495:1514	According to these results, "chemical potential gap" depicted by Flory-Huggins theory was suggested to be responsible for the filtration behaviors of alginate solution caused by calcium addition.
29169107	3	56	theme	calcium	964:970	arg1	concentration					972:984	calcium concentration	964:984	calcium concentration	964:984	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	57	theme	functional	871:880	arg1	elements					890:897	elements	890:897	elements	890:897	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	57	theme	functional	871:880	arg1	stability					911:919	thermal stability	903:919	thermal stability	903:919	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	57	theme	functional	871:880	arg1	groups					882:887	functional groups	871:887	functional groups	871:887	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	4	58	dep	Howbeit	987:993	arg1	THz-TDS					996:1002	THz-TDS	996:1002	THz-TDS	996:1002	Howbeit, THz-TDS spectra showed that calcium addition caused structural variation of alginate polymer in solution.
29169107	6	59	theme	calcium	1608:1614	arg1	addition					1616:1623	calcium addition	1608:1623	calcium addition	1608:1623	According to these results, "chemical potential gap" depicted by Flory-Huggins theory was suggested to be responsible for the filtration behaviors of alginate solution caused by calcium addition.
29169107	5	60	theme	DTF	1102:1104	arg1	results					1118:1124	DTF calculation results	1102:1124	DTF calculation results	1102:1124	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	1	61	theme	Fouling	136:142	arg1	mechanisms					144:153	Fouling mechanisms	136:153	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition	136:237	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	1	62	dep	spectroscopy	282:293	arg1	techniques					341:350	functional theory (DFT) techniques	317:350	functional theory (DFT) techniques	317:350	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	5	63	theme	alginate	1288:1295	arg1	chains					1297:1302	the two alginate chains	1280:1302	the two alginate chains bridged by a calcium atom	1280:1328	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	2	64	theme	filtration	391:400	arg1	SFR					414:416	SFR	414:416	SFR	414:416	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	2	64	theme	filtration	391:400	arg1	resistance					402:411	specific filtration resistance	382:411	specific filtration resistance (SFR) of alginate solution (0.75 g L-1)	382:451	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	5	65	theme	calcium	1317:1323	arg1	atom					1325:1328	a calcium atom	1315:1328	a calcium atom	1315:1328	DTF calculation results showed that initial binding of alginate chains induced by calcium ions preferentially occurred in intermolecular other than intramolecular, and moreover, the two alginate chains bridged by a calcium atom tend to stretch in a tetrahedron structure (cross to each other) other than parallel to each other.
29169107	2	66	theme	calcium	481:487	arg1	addition					489:496	calcium addition	481:496	calcium addition	481:496	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	3	67	theme	X-ray	716:720	arg1	XPS					750:752	XPS	750:752	XPS	750:752	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	67	theme	X-ray	716:720	arg1	Fourier					756:762	Fourier	756:762	Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA)	756:838	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	67	theme	X-ray	716:720	arg1	spectroscopy					736:747	X-ray photoelectric spectroscopy	716:747	X-ray photoelectric spectroscopy (XPS)	716:753	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	68	theme	Thermogravimetric	807:823	arg1	analysis					825:832	Thermogravimetric analysis	807:832	Thermogravimetric analysis (TGA)	807:838	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	3	68	theme	Thermogravimetric	807:823	arg1	TGA					835:837	TGA	835:837	TGA	835:837	Characterizations by X-ray photoelectric spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) showed that the composition of functional groups, elements and thermal stability of alginate was not apparently affected by calcium concentration.
29169107	2	69	theme	specific	382:389	arg1	SFR					414:416	SFR	414:416	SFR	414:416	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	2	69	theme	specific	382:389	arg1	resistance					402:411	specific filtration resistance	382:411	specific filtration resistance (SFR) of alginate solution (0.75 g L-1)	382:451	Filtration tests showed that specific filtration resistance (SFR) of alginate solution (0.75 g L-1) monotonously increased with calcium addition at a relatively low range of calcium concentration (0-1.0 mM), and SFR (2.61 × 1015 m kg-1) of alginate solution with 1.0 mM calcium addition was extremely high as compared with sludge suspension.
29169107	4	70	theme	polymer	1081:1087	arg1	variation					1059:1067	structural variation	1048:1067	structural variation of alginate polymer in solution	1048:1099	Howbeit, THz-TDS spectra showed that calcium addition caused structural variation of alginate polymer in solution.
29169107	1	71	theme	Terahertz	260:268	arg1	spectroscopy					282:293	Terahertz time-domain spectroscopy	260:293	Terahertz time-domain spectroscopy (THz-TDS)	260:303	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29169107	1	71	theme	Terahertz	260:268	arg1	THz-TDS					296:302	THz-TDS	296:302	THz-TDS	296:302	Fouling mechanisms underlying the filtration behaviors of alginate solution caused by calcium addition were investigated by Terahertz time-domain spectroscopy (THz-TDS) and density functional theory (DFT) techniques.
29108433	8	0	dep	RESULTS	990:996	arg1	samples					1009:1015	Human milk samples	998:1015	RESULTS Human milk samples	990:1015	RESULTS Human milk samples showed significantly different HMO patterns between mothers.
29108433	8	1	theme	HMO	1048:1050	arg1	patterns					1052:1059	significantly different HMO patterns	1024:1059	significantly different HMO patterns between mothers	1024:1075	RESULTS Human milk samples showed significantly different HMO patterns between mothers.
29108433	1	2	theme	important	145:153	arg1	OBJECTIVES					86:95	OBJECTIVES Human milk oligosaccharides (HMOs)	86:130	OBJECTIVES Human milk oligosaccharides (HMOs)	86:130	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	1	2	theme	important	145:153	arg1	factors					155:161	important factors	145:161	important factors in neurologic and immunologic development of neonates	145:215	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	7	3	theme	TOF/TOF	891:897	arg1	spectrometry					905:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	HMOs profiles were analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry and compared between samples collected before and after the treatments.
29108433	8	4	theme	different	1038:1046	arg1	patterns					1052:1059	significantly different HMO patterns	1024:1059	significantly different HMO patterns between mothers	1024:1075	RESULTS Human milk samples showed significantly different HMO patterns between mothers.
29108433	5	5	theme	healthy	637:643	arg1	mothers					645:651	three healthy mothers	631:651	three healthy mothers	631:651	METHODS Totally nine fresh human milk samples were collected from three healthy mothers at the first, second, and third week after delivery.
29108433	1	6	from	factors	155:161	arg1	development					193:203	neurologic and immunologic development	166:203	neurologic and immunologic development of neonates	166:215	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	7	7	theme	time-of-flight/time-of-flight	860:888	arg1	spectrometry					905:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	HMOs profiles were analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry and compared between samples collected before and after the treatments.
29108433	13	8	theme	storage	1560:1566	arg1	length					1568:1573	the storage length	1556:1573	the storage length without composition changes of HMOs after freeze-drying	1556:1629	However, the storage length without composition changes of HMOs after freeze-drying needs to be evaluated in the further studies.
29108433	4	9	dep	pasteurization	531:544	arg1	the					527:529	the	527:529	the	527:529	The purpose of this study is to analyze and compare the HMOs profiles of human milk collected before and after the pasteurization and freeze-drying.
29108433	5	10	theme	fresh	586:590	arg1	samples					603:609	nine fresh human milk samples	581:609	nine fresh human milk samples	581:609	METHODS Totally nine fresh human milk samples were collected from three healthy mothers at the first, second, and third week after delivery.
29108433	1	11	dep	OBJECTIVES	86:95	arg1	HMOs					126:129	HMOs	126:129	HMOs	126:129	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	1	11	dep	OBJECTIVES	86:95	arg1	oligosaccharides					108:123	Human milk oligosaccharides	97:123	OBJECTIVES Human milk oligosaccharides (HMOs)	86:130	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	11	12	theme	milks	1435:1439	arg1	freeze-drying					1412:1424	freeze-drying	1412:1424	freeze-drying	1412:1424	CONCLUSION HMO patterns were found not to be affected by pasteurization and freeze-drying of donor milks.
29108433	11	12	theme	milks	1435:1439	arg1	pasteurization					1393:1406	pasteurization	1393:1406	pasteurization	1393:1406	CONCLUSION HMO patterns were found not to be affected by pasteurization and freeze-drying of donor milks.
29108433	5	13	theme	milk	598:601	arg1	samples					603:609	nine fresh human milk samples	581:609	nine fresh human milk samples	581:609	METHODS Totally nine fresh human milk samples were collected from three healthy mothers at the first, second, and third week after delivery.
29108433	5	14	theme	human	592:596	arg1	samples					603:609	nine fresh human milk samples	581:609	nine fresh human milk samples	581:609	METHODS Totally nine fresh human milk samples were collected from three healthy mothers at the first, second, and third week after delivery.
29108433	13	15	theme	HMOs	1606:1609	arg1	changes					1595:1601	composition changes	1583:1601	composition changes of HMOs	1583:1609	However, the storage length without composition changes of HMOs after freeze-drying needs to be evaluated in the further studies.
29108433	7	16	theme	MALDI	853:857	arg1	spectrometry					905:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	HMOs profiles were analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry and compared between samples collected before and after the treatments.
29108433	12	17	theme	present	1532:1538	arg1	study					1540:1544	the present study	1528:1544	the present study	1528:1544	We hope that introducing freeze-drying to the human milk banks would be encouraged by the present study.
29108433	2	18	theme	storage	266:272	arg1	freeze-drying					228:240	freeze-drying	228:240	freeze-drying	228:240	Moreover, freeze-drying seems to be a promising storage method to improve the processes of human milk banks.
29108433	2	18	theme	storage	266:272	arg1	method					274:279	a promising storage method	254:279	a promising storage method to improve the processes of human milk banks	254:324	Moreover, freeze-drying seems to be a promising storage method to improve the processes of human milk banks.
29108433	7	19	theme	laser	824:828	arg1	spectrometry					905:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	HMOs profiles were analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry and compared between samples collected before and after the treatments.
29108433	1	20	theme	neurologic	166:175	arg1	development					193:203	neurologic and immunologic development	166:203	neurologic and immunologic development of neonates	166:215	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	0	21	theme	human	4:8	arg1	oligosaccharides					15:30	The human milk oligosaccharides	0:30	The human milk oligosaccharides	0:30	The human milk oligosaccharides are not affected by pasteurization and freeze-drying.
29108433	8	22	theme	milk	1004:1007	arg1	samples					1009:1015	Human milk samples	998:1015	RESULTS Human milk samples	990:1015	RESULTS Human milk samples showed significantly different HMO patterns between mothers.
29108433	2	23	theme	human	309:313	arg1	banks					320:324	human milk banks	309:324	human milk banks	309:324	Moreover, freeze-drying seems to be a promising storage method to improve the processes of human milk banks.
29108433	7	24	theme	desorption/ionization	830:850	arg1	spectrometry					905:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	HMOs profiles were analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry and compared between samples collected before and after the treatments.
29108433	11	25	theme	HMO	1347:1349	arg1	patterns					1351:1358	CONCLUSION HMO patterns	1336:1358	CONCLUSION HMO patterns	1336:1358	CONCLUSION HMO patterns were found not to be affected by pasteurization and freeze-drying of donor milks.
29108433	7	26	theme	HMOs	777:780	arg1	profiles					782:789	HMOs profiles	777:789	HMOs profiles	777:789	HMOs profiles were analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry and compared between samples collected before and after the treatments.
29108433	6	27	theme	Holder	736:741	arg1	pasteurization					743:756	Holder pasteurization	736:756	Holder pasteurization	736:756	The samples were treated with Holder pasteurization and freeze-drying.
29108433	1	28	theme	Human	97:101	arg1	HMOs					126:129	HMOs	126:129	HMOs	126:129	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	1	28	theme	Human	97:101	arg1	oligosaccharides					108:123	Human milk oligosaccharides	97:123	OBJECTIVES Human milk oligosaccharides (HMOs)	86:130	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	1	29	theme	immunologic	181:191	arg1	development					193:203	neurologic and immunologic development	166:203	neurologic and immunologic development of neonates	166:215	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	3	30	theme	pasteurization	351:364	arg1	effects					340:346	the effects	336:346	the effects of pasteurization and freeze-drying on HMOs	336:390	However, the effects of pasteurization and freeze-drying on HMOs were not evaluated yet.
29108433	5	31	theme	second	667:672	arg1	week					685:688	the first, second, and third week	656:688	the first, second, and third week after delivery	656:703	METHODS Totally nine fresh human milk samples were collected from three healthy mothers at the first, second, and third week after delivery.
29108433	1	32	theme	milk	103:106	arg1	HMOs					126:129	HMOs	126:129	HMOs	126:129	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	1	32	theme	milk	103:106	arg1	oligosaccharides					108:123	Human milk oligosaccharides	97:123	OBJECTIVES Human milk oligosaccharides (HMOs)	86:130	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	7	33	theme	matrix-assisted	808:822	arg1	spectrometry					905:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	HMOs profiles were analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry and compared between samples collected before and after the treatments.
29108433	0	34	theme	milk	10:13	arg1	oligosaccharides					15:30	The human milk oligosaccharides	0:30	The human milk oligosaccharides	0:30	The human milk oligosaccharides are not affected by pasteurization and freeze-drying.
29108433	13	35	theme	composition	1583:1593	arg1	changes					1595:1601	composition changes	1583:1601	composition changes of HMOs	1583:1609	However, the storage length without composition changes of HMOs after freeze-drying needs to be evaluated in the further studies.
29108433	10	36	theme	correlation	1285:1295	arg1	analysis					1297:1304	a correlation analysis	1283:1304	a correlation analysis (r2 = 0.989-0.999, p < .001)	1283:1333	Moreover, both of pasteurization and freeze-drying were found not to affect HMO patterns in a correlation analysis (r2 = 0.989-0.999, p < .001).
29108433	9	37	dep	r2 = 0.972-0.999	1162:1177	arg1	p < .001					1180:1187	p < .001	1180:1187	p < .001	1180:1187	However, HMOs were not affected by lactation periods within 3 weeks after delivery (r2 = 0.972-0.999, p < .001).
29108433	1	38	theme	neonates	208:215	arg1	development					193:203	neurologic and immunologic development	166:203	neurologic and immunologic development of neonates	166:215	OBJECTIVES Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates.
29108433	4	39	theme	milk	495:498	arg1	profiles					477:484	the HMOs profiles	468:484	the HMOs profiles of human milk collected before and after the pasteurization and freeze-drying	468:562	The purpose of this study is to analyze and compare the HMOs profiles of human milk collected before and after the pasteurization and freeze-drying.
29108433	5	40	theme	third	679:683	arg1	week					685:688	the first, second, and third week	656:688	the first, second, and third week after delivery	656:703	METHODS Totally nine fresh human milk samples were collected from three healthy mothers at the first, second, and third week after delivery.
29108433	3	41	from	effects	340:346	arg1	HMOs					387:390	HMOs	387:390	HMOs	387:390	However, the effects of pasteurization and freeze-drying on HMOs were not evaluated yet.
29108433	4	42	theme	human	489:493	arg1	milk					495:498	human milk	489:498	human milk collected before and after the pasteurization and freeze-drying	489:562	The purpose of this study is to analyze and compare the HMOs profiles of human milk collected before and after the pasteurization and freeze-drying.
29108433	4	43	theme	study	436:440	arg1	purpose					420:426	The purpose	416:426	The purpose of this study	416:440	The purpose of this study is to analyze and compare the HMOs profiles of human milk collected before and after the pasteurization and freeze-drying.
29108433	9	44	dep	affected	1101:1108	arg1	r2 = 0.972-0.999					1162:1177	r2 = 0.972-0.999	1162:1177	r2 = 0.972-0.999	1162:1177	However, HMOs were not affected by lactation periods within 3 weeks after delivery (r2 = 0.972-0.999, p < .001).
29108433	8	45	theme	Human	998:1002	arg1	samples					1009:1015	Human milk samples	998:1015	RESULTS Human milk samples	990:1015	RESULTS Human milk samples showed significantly different HMO patterns between mothers.
29108433	9	46	theme	lactation	1113:1121	arg1	periods					1123:1129	lactation periods	1113:1129	lactation periods	1113:1129	However, HMOs were not affected by lactation periods within 3 weeks after delivery (r2 = 0.972-0.999, p < .001).
29108433	5	47	theme	first	660:664	arg1	week					685:688	the first, second, and third week	656:688	the first, second, and third week after delivery	656:703	METHODS Totally nine fresh human milk samples were collected from three healthy mothers at the first, second, and third week after delivery.
29108433	2	48	theme	promising	256:264	arg1	freeze-drying					228:240	freeze-drying	228:240	freeze-drying	228:240	Moreover, freeze-drying seems to be a promising storage method to improve the processes of human milk banks.
29108433	2	48	theme	promising	256:264	arg1	method					274:279	a promising storage method	254:279	a promising storage method to improve the processes of human milk banks	254:324	Moreover, freeze-drying seems to be a promising storage method to improve the processes of human milk banks.
29108433	2	49	theme	banks	320:324	arg1	processes					296:304	the processes	292:304	the processes of human milk banks	292:324	Moreover, freeze-drying seems to be a promising storage method to improve the processes of human milk banks.
29108433	10	50	from	patterns	1271:1278	arg1	analysis					1297:1304	a correlation analysis	1283:1304	a correlation analysis (r2 = 0.989-0.999, p < .001)	1283:1333	Moreover, both of pasteurization and freeze-drying were found not to affect HMO patterns in a correlation analysis (r2 = 0.989-0.999, p < .001).
29108433	11	51	theme	CONCLUSION	1336:1345	arg1	patterns					1351:1358	CONCLUSION HMO patterns	1336:1358	CONCLUSION HMO patterns	1336:1358	CONCLUSION HMO patterns were found not to be affected by pasteurization and freeze-drying of donor milks.
29108433	12	52	theme	milk	1494:1497	arg1	banks					1499:1503	the human milk banks	1484:1503	the human milk banks	1484:1503	We hope that introducing freeze-drying to the human milk banks would be encouraged by the present study.
29108433	2	53	theme	milk	315:318	arg1	banks					320:324	human milk banks	309:324	human milk banks	309:324	Moreover, freeze-drying seems to be a promising storage method to improve the processes of human milk banks.
29108433	11	54	theme	donor	1429:1433	arg1	milks					1435:1439	donor milks	1429:1439	donor milks	1429:1439	CONCLUSION HMO patterns were found not to be affected by pasteurization and freeze-drying of donor milks.
29108433	12	55	theme	human	1488:1492	arg1	banks					1499:1503	the human milk banks	1484:1503	the human milk banks	1484:1503	We hope that introducing freeze-drying to the human milk banks would be encouraged by the present study.
29108433	13	56	theme	further	1660:1666	arg1	studies					1668:1674	the further studies	1656:1674	the further studies	1656:1674	However, the storage length without composition changes of HMOs after freeze-drying needs to be evaluated in the further studies.
29108433	7	57	theme	mass	900:903	arg1	spectrometry					905:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry	808:916	HMOs profiles were analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry and compared between samples collected before and after the treatments.
29108433	4	58	theme	HMOs	472:475	arg1	profiles					477:484	the HMOs profiles	468:484	the HMOs profiles of human milk collected before and after the pasteurization and freeze-drying	468:562	The purpose of this study is to analyze and compare the HMOs profiles of human milk collected before and after the pasteurization and freeze-drying.
29108433	10	59	dep	analysis	1297:1304	arg1	r2 = 0.989-0.999					1307:1322	r2 = 0.989-0.999	1307:1322	r2 = 0.989-0.999	1307:1322	Moreover, both of pasteurization and freeze-drying were found not to affect HMO patterns in a correlation analysis (r2 = 0.989-0.999, p < .001).
29108433	10	59	dep	analysis	1297:1304	arg1	p < .001					1325:1332	p < .001	1325:1332	p < .001	1325:1332	Moreover, both of pasteurization and freeze-drying were found not to affect HMO patterns in a correlation analysis (r2 = 0.989-0.999, p < .001).
29108433	3	60	theme	freeze-drying	370:382	arg1	effects					340:346	the effects	336:346	the effects of pasteurization and freeze-drying on HMOs	336:390	However, the effects of pasteurization and freeze-drying on HMOs were not evaluated yet.
29108433	10	61	theme	HMO	1267:1269	arg1	patterns					1271:1278	HMO patterns	1267:1278	HMO patterns in a correlation analysis (r2 = 0.989-0.999, p < .001)	1267:1333	Moreover, both of pasteurization and freeze-drying were found not to affect HMO patterns in a correlation analysis (r2 = 0.989-0.999, p < .001).
28034292	6	0	theme	modified	940:947	arg1	medium					953:958	the modified RCM medium	936:958	the modified RCM medium	936:958	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	6	0	theme	modified	940:947	arg1	medium					925:930	Experimental medium	912:930	Experimental medium	912:930	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	4	1	theme	99	549:550	arg1	%					551:551	%	551:551	%	551:551	Food grade pullulan 99% purity.
28034292	5	2	theme	standard	604:611	arg1	RCM					613:615	the first standard RCM	594:615	the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM	594:817	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	2	3	theme	pullulan	307:314	arg1	effect					297:302	the effect	293:302	the effect of pullulan on the growth and fermentation activity of selected human intestinal bacteria	293:392	The objective of the study was to determine the effect of pullulan on the growth and fermentation activity of selected human intestinal bacteria.
28034292	3	4	theme	experimental	506:517	arg1	material					519:526	experimental material	506:526	experimental material	506:526	METHODS Commercial pullulan purchased from Focubase (China) of a molecular weight of 100,000 Da constituted as experimental material.
28034292	2	5	from	effect	297:302	arg1	activity					347:354	the growth and fermentation activity	319:354	the growth and fermentation activity of selected human intestinal bacteria	319:392	The objective of the study was to determine the effect of pullulan on the growth and fermentation activity of selected human intestinal bacteria.
28034292	9	6	with	tubes	1325:1329	arg1	sachets					1354:1360	AnaeroGenTM 2.5 l sachets	1336:1360	AnaeroGenTM 2.5 l sachets	1336:1360	After mixing, flasks were placed in anaerobic tubes with AnaeroGenTM 2.5 l sachets.
28034292	16	7	theme	E.	2534:2535	arg1	bacteria					2542:2549	E. coli bacteria	2534:2549	E. coli bacteria	2534:2549	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	1	8	theme	energy	157:162	arg1	value					164:168	low energy value	153:168	low energy value	153:168	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	16	9	theme	increased	2444:2452	arg1	cause					2498:2502	the cause	2494:2502	the cause of reduction in the number of E. coli bacteria	2494:2549	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	16	9	theme	increased	2444:2452	arg1	activity					2465:2472	their increased acidifying activity	2438:2472	their increased acidifying activity	2438:2472	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	12	10	theme	total	1795:1799	arg1	acidity					1801:1807	Higher total acidity	1788:1807	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media	1788:1878	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	16	11	theme	bacteria	2542:2549	arg1	number					2524:2529	the number	2520:2529	the number of E. coli bacteria	2520:2549	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	10	12	theme	bacteria	1494:1501	arg1	number					1431:1436	The number	1427:1436	The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria	1427:1501	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	10	12	theme	bacteria	1494:1501	arg1	pH					1515:1516	pH	1515:1516	pH	1515:1516	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	8	13	from	volume	1207:1212	arg1	suspensions					1190:1200	suspensions	1190:1200	suspensions at a volume of 300 μl	1190:1222	Then, suspensions at a volume of 300 μl were transferred to the media (RCM, RCM+G, and RCM+P).
28034292	12	14	dep	acidity	1801:1807	arg1	ml					1821:1822	ml	1821:1822	ml	1821:1822	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	16	15	from	number	2524:2529	arg1	cause					2498:2502	the cause	2494:2502	the cause of reduction in the number of E. coli bacteria	2494:2549	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	16	15	from	number	2524:2529	arg1	activity					2465:2472	their increased acidifying activity	2438:2472	their increased acidifying activity	2438:2472	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	15	16	theme	natural	2288:2294	arg1	microflora					2296:2305	the natural microflora	2284:2305	the natural microflora of the infants' colon	2284:2327	CONCLUSION In summary, pullulan exhibited a selective effect on the natural microflora of the infants' colon.
28034292	10	17	theme	Escherichia	1477:1487	arg1	bacteria					1494:1501	Escherichia coli bacteria	1477:1501	Escherichia coli bacteria	1477:1501	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	5	18	theme	sodium	728:733	arg1	acetate					735:741	0.3 sodium acetate	724:741	0.3 sodium acetate	724:741	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	15	19	from	effect	2274:2279	arg1	microflora					2296:2305	the natural microflora	2284:2305	the natural microflora of the infants' colon	2284:2327	CONCLUSION In summary, pullulan exhibited a selective effect on the natural microflora of the infants' colon.
28034292	16	20	theme	pullulan	2364:2371	arg1	effect					2354:2359	no stimulating effect	2339:2359	no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus	2339:2422	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	1	21	from	component	181:189	arg1	snacks					227:232	dietary snacks	219:232	dietary snacks for diabetics	219:246	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	11	22	dep	Bifidobacterium	1638:1652	arg1	the					1634:1636	the	1634:1636	the	1634:1636	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	8	23	theme	μl	1221:1222	arg1	volume					1207:1212	a volume	1205:1212	a volume of 300 μl	1205:1222	Then, suspensions at a volume of 300 μl were transferred to the media (RCM, RCM+G, and RCM+P).
28034292	16	24	theme	reduction	2507:2515	arg1	cause					2498:2502	the cause	2494:2502	the cause of reduction in the number of E. coli bacteria	2494:2549	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	16	24	theme	reduction	2507:2515	arg1	activity					2465:2472	their increased acidifying activity	2438:2472	their increased acidifying activity	2438:2472	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	1	25	theme	BACKGROUND	100:109	arg1	Pullulan					111:118	BACKGROUND Pullulan	100:118	BACKGROUND Pullulan	100:118	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	1	25	theme	BACKGROUND	100:109	arg1	polysaccharide					135:148	a microbial polysaccharide	123:148	a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics	123:246	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	5	26	dep	wherein	820:826	arg1	starch					840:845	the soluble starch	828:845	wherein the soluble starch	820:845	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	11	27	theme	Bifidobacterium	1638:1652	arg1	numbers					1611:1617	the numbers	1607:1617	the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan	1607:1700	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	11	27	theme	Bifidobacterium	1638:1652	arg1	lower					1729:1733	lower	1729:1733	lower	1729:1733	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	16	28	from	effect	2354:2359	arg1	growth					2380:2385	the growth	2376:2385	the growth of Bifidobacterium and Lactobacillus	2376:2422	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	14	29	theme	gut	2204:2206	arg1	microbiota					2208:2217	gut microbiota	2204:2217	gut microbiota	2204:2217	Increase in the fermentation activity of bacteria in medium with pullulan favorably influenced modification of the composition of gut microbiota.
28034292	12	30	theme	lower	1923:1927	arg1	4.15					1969:1972	4.15	1969:1972	4.15	1969:1972	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	12	30	theme	lower	1923:1927	arg1	4.70					1978:1981	4.70	1978:1981	4.70	1978:1981	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	12	30	theme	lower	1923:1927	arg1	values					1932:1937	lower pH values	1923:1937	lower pH values than RCM medium	1923:1953	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	0	31	theme	Acidifying	41:50	arg1	Activity					52:59	Acidifying Activity	41:59	Acidifying Activity	41:59	The Effect of Pullulan on the Growth and Acidifying Activity of Selected Stool Microflora of Human.
28034292	12	32	theme	RCM	1944:1946	arg1	medium					1948:1953	RCM medium	1944:1953	RCM medium	1944:1953	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	15	33	theme	selective	2264:2272	arg1	effect					2274:2279	a selective effect	2262:2279	a selective effect on the natural microflora of the infants' colon	2262:2327	CONCLUSION In summary, pullulan exhibited a selective effect on the natural microflora of the infants' colon.
28034292	1	34	theme	microbial	125:133	arg1	Pullulan					111:118	BACKGROUND Pullulan	100:118	BACKGROUND Pullulan	100:118	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	1	34	theme	microbial	125:133	arg1	polysaccharide					135:148	a microbial polysaccharide	123:148	a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics	123:246	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	14	35	theme	composition	2189:2199	arg1	modification					2169:2180	modification	2169:2180	modification of the composition of gut microbiota	2169:2217	Increase in the fermentation activity of bacteria in medium with pullulan favorably influenced modification of the composition of gut microbiota.
28034292	12	36	theme	control	1866:1872	arg1	media					1874:1878	the control media	1862:1878	the control media	1862:1878	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	0	37	theme	Stool	73:77	arg1	Microflora					79:88	Selected Stool Microflora	64:88	Selected Stool Microflora of Human	64:97	The Effect of Pullulan on the Growth and Acidifying Activity of Selected Stool Microflora of Human.
28034292	16	38	theme	Lactobacillus	2410:2422	arg1	growth					2380:2385	the growth	2376:2385	the growth of Bifidobacterium and Lactobacillus	2376:2422	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	5	39	theme	control	565:571	arg1	media					573:577	Two control media	561:577	Two control media	561:577	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	3	40	theme	Commercial	403:412	arg1	pullulan					414:421	METHODS Commercial pullulan	395:421	METHODS Commercial pullulan purchased from Focubase (China) of a molecular weight of 100,000 Da	395:489	METHODS Commercial pullulan purchased from Focubase (China) of a molecular weight of 100,000 Da constituted as experimental material.
28034292	5	41	dep	RCM	613:615	arg1	extract					696:702	1.0 peptone, 1.0 meat extract	674:702	extract	696:702	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	5	41	dep	RCM	613:615	arg1	glucose					645:651	0.5 glucose	641:651	0.5 glucose	641:651	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	5	41	dep	RCM	613:615	arg1	acetate					735:741	0.3 sodium acetate	724:741	0.3 sodium acetate	724:741	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	5	41	dep	RCM	613:615	arg1	NaCl					777:780	0.5 NaCl	773:780	0.5 NaCl	773:780	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	5	41	dep	RCM	613:615	arg1	pH					783:784	pH 6.8	783:788	pH 6.8	783:788	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	5	41	dep	RCM	613:615	arg1	extract					715:721	0.3 yeast extract	705:721	0.3 yeast extract	705:721	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	5	41	dep	RCM	613:615	arg1	RCM					815:817	the second modified RCM	795:817	the second modified RCM	795:817	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	5	41	dep	RCM	613:615	arg1	starch					666:671	0.1 soluble starch	654:671	0.1 soluble starch	654:671	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	5	41	dep	RCM	613:615	arg1	hydrochloride					758:770	0.05 cysteine hydrochloride	744:770	0.05 cysteine hydrochloride	744:770	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	2	42	theme	fermentation	334:345	arg1	activity					347:354	the growth and fermentation activity	319:354	the growth and fermentation activity of selected human intestinal bacteria	319:392	The objective of the study was to determine the effect of pullulan on the growth and fermentation activity of selected human intestinal bacteria.
28034292	5	43	theme	yeast	709:713	arg1	extract					715:721	0.3 yeast extract	705:721	0.3 yeast extract	705:721	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	8	44	dep	media	1248:1252	arg1	RCM+G					1260:1264	RCM+G	1260:1264	RCM+G	1260:1264	Then, suspensions at a volume of 300 μl were transferred to the media (RCM, RCM+G, and RCM+P).
28034292	8	44	dep	media	1248:1252	arg1	RCM+P					1271:1275	RCM+P	1271:1275	RCM+P	1271:1275	Then, suspensions at a volume of 300 μl were transferred to the media (RCM, RCM+G, and RCM+P).
28034292	8	44	dep	media	1248:1252	arg1	RCM					1255:1257	RCM	1255:1257	RCM	1255:1257	Then, suspensions at a volume of 300 μl were transferred to the media (RCM, RCM+G, and RCM+P).
28034292	8	44	dep	media	1248:1252	arg1	media					1248:1252	the media	1244:1252	the media (RCM, RCM+G, and RCM+P)	1244:1276	Then, suspensions at a volume of 300 μl were transferred to the media (RCM, RCM+G, and RCM+P).
28034292	5	45	theme	modified	806:813	arg1	RCM					815:817	the second modified RCM	795:817	the second modified RCM	795:817	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	10	46	theme	total	1522:1526	arg1	acidity					1528:1534	total acidity	1522:1534	total acidity of the culture during 0, 24, and 48 h	1522:1572	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	5	47	theme	glucose	873:879	arg1	concentration					881:893	increased glucose concentration	863:893	increased glucose concentration to 2.0% (RCM+G)	863:909	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	2	48	theme	bacteria	385:392	arg1	activity					347:354	the growth and fermentation activity	319:354	the growth and fermentation activity of selected human intestinal bacteria	319:392	The objective of the study was to determine the effect of pullulan on the growth and fermentation activity of selected human intestinal bacteria.
28034292	10	49	theme	Lactobacillus	1458:1470	arg1	number					1431:1436	The number	1427:1436	The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria	1427:1501	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	10	49	theme	Lactobacillus	1458:1470	arg1	pH					1515:1516	pH	1515:1516	pH	1515:1516	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	4	50	theme	Food	529:532	arg1	grade					534:538	Food grade	529:538	Food grade	529:538	Food grade pullulan 99% purity.
28034292	2	51	theme	human	368:372	arg1	bacteria					385:392	selected human intestinal bacteria	359:392	selected human intestinal bacteria	359:392	The objective of the study was to determine the effect of pullulan on the growth and fermentation activity of selected human intestinal bacteria.
28034292	5	52	theme	meat	691:694	arg1	extract					696:702	1.0 peptone, 1.0 meat extract	674:702	extract	696:702	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	16	53	theme	stimulating	2342:2352	arg1	effect					2354:2359	no stimulating effect	2339:2359	no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus	2339:2422	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	12	54	theme	pullulan	1828:1835	arg1	culture					1837:1843	pullulan culture	1828:1843	pullulan culture	1828:1843	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	9	55	theme	AnaeroGenTM	1336:1346	arg1	sachets					1354:1360	AnaeroGenTM 2.5 l sachets	1336:1360	AnaeroGenTM 2.5 l sachets	1336:1360	After mixing, flasks were placed in anaerobic tubes with AnaeroGenTM 2.5 l sachets.
28034292	7	56	theme	stool	1105:1109	arg1	samples					1111:1117	fresh stool samples	1099:1117	fresh stool samples (1 g)	1099:1123	Stool suspensions were prepared from fresh stool samples (1 g) in peptone water (9 g), which were previously homogenized.
28034292	7	56	theme	stool	1105:1109	arg1	g					1122:1122	1 g	1120:1122	1 g	1120:1122	Stool suspensions were prepared from fresh stool samples (1 g) in peptone water (9 g), which were previously homogenized.
28034292	11	57	from	medium	1681:1686	arg1	numbers					1611:1617	the numbers	1607:1617	the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan	1607:1700	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	11	57	from	medium	1681:1686	arg1	lower					1729:1733	lower	1729:1733	lower	1729:1733	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	6	58	dep	wherein	961:967	arg1	glucose					992:998	glucose	992:998	glucose	992:998	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	6	58	dep	wherein	961:967	arg1	starch					981:986	soluble starch	973:986	soluble starch	973:986	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	1	59	theme	low-calorie	194:204	arg1	foods					206:210	low-calorie foods	194:210	low-calorie foods	194:210	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	9	60	theme	l	1352:1352	arg1	sachets					1354:1360	AnaeroGenTM 2.5 l sachets	1336:1360	AnaeroGenTM 2.5 l sachets	1336:1360	After mixing, flasks were placed in anaerobic tubes with AnaeroGenTM 2.5 l sachets.
28034292	12	61	theme	g/100	1815:1819	arg1	ml					1821:1822	ml	1821:1822	ml	1821:1822	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	15	62	dep	CONCLUSION	2220:2229	arg1	summary					2234:2240	summary	2234:2240	summary	2234:2240	CONCLUSION In summary, pullulan exhibited a selective effect on the natural microflora of the infants' colon.
28034292	10	63	theme	Bifidobacterium	1441:1455	arg1	number					1431:1436	The number	1427:1436	The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria	1427:1501	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	10	63	theme	Bifidobacterium	1441:1455	arg1	pH					1515:1516	pH	1515:1516	pH	1515:1516	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	5	64	theme	cysteine	749:756	arg1	hydrochloride					758:770	0.05 cysteine hydrochloride	744:770	0.05 cysteine hydrochloride	744:770	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	14	65	theme	fermentation	2090:2101	arg1	activity					2103:2110	the fermentation activity	2086:2110	the fermentation activity of bacteria in medium with pullulan	2086:2146	Increase in the fermentation activity of bacteria in medium with pullulan favorably influenced modification of the composition of gut microbiota.
28034292	5	66	theme	increased	863:871	arg1	concentration					881:893	increased glucose concentration	863:893	increased glucose concentration to 2.0% (RCM+G)	863:909	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	6	67	theme	RCM	949:951	arg1	medium					953:958	the modified RCM medium	936:958	the modified RCM medium	936:958	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	6	67	theme	RCM	949:951	arg1	medium					925:930	Experimental medium	912:930	Experimental medium	912:930	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	5	68	theme	peptone	678:684	arg1	extract					696:702	1.0 peptone, 1.0 meat extract	674:702	extract	696:702	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	5	69	theme	first	598:602	arg1	RCM					613:615	the first standard RCM	594:615	the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM	594:817	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	3	70	theme	molecular	460:468	arg1	weight					470:475	a molecular weight	458:475	a molecular weight of 100,000 Da	458:489	METHODS Commercial pullulan purchased from Focubase (China) of a molecular weight of 100,000 Da constituted as experimental material.
28034292	1	71	theme	dietary	219:225	arg1	snacks					227:232	dietary snacks	219:232	dietary snacks for diabetics	219:246	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	11	72	theme	control	1773:1779	arg1	media					1781:1785	the control media	1769:1785	the control media	1769:1785	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	7	73	theme	Stool	1062:1066	arg1	suspensions					1068:1078	Stool suspensions	1062:1078	Stool suspensions	1062:1078	Stool suspensions were prepared from fresh stool samples (1 g) in peptone water (9 g), which were previously homogenized.
28034292	11	74	theme	genus	1672:1676	arg1	numbers					1611:1617	the numbers	1607:1617	the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan	1607:1700	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	11	74	theme	genus	1672:1676	arg1	lower					1729:1733	lower	1729:1733	lower	1729:1733	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	12	75	from	acidity	1801:1807	arg1	comparison					1848:1857	comparison	1848:1857	comparison to the control media	1848:1878	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	14	76	with	medium	2127:2132	arg1	pullulan					2139:2146	pullulan	2139:2146	pullulan	2139:2146	Increase in the fermentation activity of bacteria in medium with pullulan favorably influenced modification of the composition of gut microbiota.
28034292	4	77	theme	%	551:551	arg1	purity					553:558	99% purity	549:558	99% purity	549:558	Food grade pullulan 99% purity.
28034292	7	78	theme	fresh	1099:1103	arg1	samples					1111:1117	fresh stool samples	1099:1117	fresh stool samples (1 g)	1099:1123	Stool suspensions were prepared from fresh stool samples (1 g) in peptone water (9 g), which were previously homogenized.
28034292	7	78	theme	fresh	1099:1103	arg1	g					1122:1122	1 g	1120:1122	1 g	1120:1122	Stool suspensions were prepared from fresh stool samples (1 g) in peptone water (9 g), which were previously homogenized.
28034292	3	79	theme	Da	488:489	arg1	weight					470:475	a molecular weight	458:475	a molecular weight of 100,000 Da	458:489	METHODS Commercial pullulan purchased from Focubase (China) of a molecular weight of 100,000 Da constituted as experimental material.
28034292	1	80	theme	low	153:155	arg1	value					164:168	low energy value	153:168	low energy value	153:168	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	6	81	dep	starch	981:986	arg1	the					969:971	the	969:971	the	969:971	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	11	82	dep	numbers	1758:1764	arg1	comparison					1738:1747	comparison	1738:1747	comparison	1738:1747	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	1	83	theme	value	164:168	arg1	Pullulan					111:118	BACKGROUND Pullulan	100:118	BACKGROUND Pullulan	100:118	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	1	83	theme	value	164:168	arg1	polysaccharide					135:148	a microbial polysaccharide	123:148	a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics	123:246	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	12	84	theme	Higher	1788:1793	arg1	acidity					1801:1807	Higher total acidity	1788:1807	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media	1788:1878	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	2	85	theme	study	270:274	arg1	objective					253:261	The objective	249:261	The objective of the study	249:274	The objective of the study was to determine the effect of pullulan on the growth and fermentation activity of selected human intestinal bacteria.
28034292	2	86	theme	growth	323:328	arg1	activity					347:354	the growth and fermentation activity	319:354	the growth and fermentation activity of selected human intestinal bacteria	319:392	The objective of the study was to determine the effect of pullulan on the growth and fermentation activity of selected human intestinal bacteria.
28034292	16	87	theme	acidifying	2454:2463	arg1	cause					2498:2502	the cause	2494:2502	the cause of reduction in the number of E. coli bacteria	2494:2549	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	16	87	theme	acidifying	2454:2463	arg1	activity					2465:2472	their increased acidifying activity	2438:2472	their increased acidifying activity	2438:2472	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	11	88	from	numbers	1611:1617	arg1	medium					1681:1686	medium	1681:1686	medium with pullulan	1681:1700	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	11	89	theme	logarithmic	1711:1721	arg1	cycle					1723:1727	one logarithmic cycle	1707:1727	one logarithmic cycle	1707:1727	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	6	90	theme	%	1051:1051	arg1	concentration					1031:1043	a concentration	1029:1043	a concentration of 2.0% (RCM+P)	1029:1059	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	10	91	dep	Escherichia	1477:1487	arg1	coli					1489:1492	coli	1489:1492	coli	1489:1492	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	13	92	theme	colon	2067:2071	arg1	microflora					2049:2058	the natural microflora	2037:2058	the natural microflora of the colon	2037:2071	Pullulan exhibited selective effect on the natural microflora of the colon.
28034292	14	93	theme	microbiota	2208:2217	arg1	composition					2189:2199	the composition	2185:2199	the composition of gut microbiota	2185:2217	Increase in the fermentation activity of bacteria in medium with pullulan favorably influenced modification of the composition of gut microbiota.
28034292	12	94	theme	pH	1929:1930	arg1	4.15					1969:1972	4.15	1969:1972	4.15	1969:1972	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	12	94	theme	pH	1929:1930	arg1	4.70					1978:1981	4.70	1978:1981	4.70	1978:1981	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	12	94	theme	pH	1929:1930	arg1	values					1932:1937	lower pH values	1923:1937	lower pH values than RCM medium	1923:1953	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	11	95	theme	bacteria	1622:1629	arg1	numbers					1611:1617	the numbers	1607:1617	the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan	1607:1700	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	11	95	theme	bacteria	1622:1629	arg1	lower					1729:1733	lower	1729:1733	lower	1729:1733	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	16	96	theme	Bifidobacterium	2390:2404	arg1	growth					2380:2385	the growth	2376:2385	the growth of Bifidobacterium and Lactobacillus	2376:2422	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	6	97	from	concentration	1031:1043	arg1	pullulan					1017:1024	pullulan	1017:1024	pullulan at a concentration of 2.0% (RCM+P)	1017:1059	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	0	98	theme	Selected	64:71	arg1	Microflora					79:88	Selected Stool Microflora	64:88	Selected Stool Microflora of Human	64:97	The Effect of Pullulan on the Growth and Acidifying Activity of Selected Stool Microflora of Human.
28034292	16	99	from	reduction	2507:2515	arg1	number					2524:2529	the number	2520:2529	the number of E. coli bacteria	2520:2549	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	0	100	from	Effect	4:9	arg1	Growth					30:35	Growth	30:35	Growth	30:35	The Effect of Pullulan on the Growth and Acidifying Activity of Selected Stool Microflora of Human.
28034292	0	100	from	Effect	4:9	arg1	Activity					52:59	Acidifying Activity	41:59	Acidifying Activity	41:59	The Effect of Pullulan on the Growth and Acidifying Activity of Selected Stool Microflora of Human.
28034292	14	101	from	Increase	2074:2081	arg1	activity					2103:2110	the fermentation activity	2086:2110	the fermentation activity of bacteria in medium with pullulan	2086:2146	Increase in the fermentation activity of bacteria in medium with pullulan favorably influenced modification of the composition of gut microbiota.
28034292	0	102	theme	Microflora	79:88	arg1	Growth					30:35	Growth	30:35	Growth	30:35	The Effect of Pullulan on the Growth and Acidifying Activity of Selected Stool Microflora of Human.
28034292	0	102	theme	Microflora	79:88	arg1	Activity					52:59	Acidifying Activity	41:59	Acidifying Activity	41:59	The Effect of Pullulan on the Growth and Acidifying Activity of Selected Stool Microflora of Human.
28034292	16	103	from	cause	2498:2502	arg1	number					2524:2529	the number	2520:2529	the number of E. coli bacteria	2520:2549	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	16	104	dep	E.	2534:2535	arg1	coli					2537:2540	coli	2537:2540	coli	2537:2540	Although no stimulating effect of pullulan on the growth of Bifidobacterium and Lactobacillus was observed, their increased acidifying activity, which probably was the cause of reduction in the number of E. coli bacteria, was confirmed.
28034292	0	105	theme	Human	93:97	arg1	Microflora					79:88	Selected Stool Microflora	64:88	Selected Stool Microflora of Human	64:97	The Effect of Pullulan on the Growth and Acidifying Activity of Selected Stool Microflora of Human.
28034292	5	106	theme	soluble	832:838	arg1	starch					840:845	the soluble starch	828:845	wherein the soluble starch	820:845	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	7	107	theme	peptone	1128:1134	arg1	g					1145:1145	9 g	1143:1145	9 g	1143:1145	Stool suspensions were prepared from fresh stool samples (1 g) in peptone water (9 g), which were previously homogenized.
28034292	7	107	theme	peptone	1128:1134	arg1	water					1136:1140	peptone water	1128:1140	peptone water (9 g)	1128:1146	Stool suspensions were prepared from fresh stool samples (1 g) in peptone water (9 g), which were previously homogenized.
28034292	10	108	theme	samples	1377:1383	arg1	Incubation					1363:1372	Incubation	1363:1372	Incubation of samples	1363:1383	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	11	109	with	medium	1681:1686	arg1	pullulan					1693:1700	pullulan	1693:1700	pullulan	1693:1700	It was found that the numbers of bacteria of the Bifidobacterium and Lactobacillus genus in medium with pullulan were one logarithmic cycle lower in comparison to their numbers in the control media.
28034292	5	110	theme	second	799:804	arg1	RCM					815:817	the second modified RCM	795:817	the second modified RCM	795:817	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	6	111	theme	Experimental	912:923	arg1	medium					953:958	the modified RCM medium	936:958	the modified RCM medium	936:958	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	6	111	theme	Experimental	912:923	arg1	medium					925:930	Experimental medium	912:930	Experimental medium	912:930	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	12	112	theme	culture	1837:1843	arg1	acidity					1801:1807	Higher total acidity	1788:1807	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media	1788:1878	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	3	113	theme	METHODS	395:401	arg1	pullulan					414:421	METHODS Commercial pullulan	395:421	METHODS Commercial pullulan purchased from Focubase (China) of a molecular weight of 100,000 Da	395:489	METHODS Commercial pullulan purchased from Focubase (China) of a molecular weight of 100,000 Da constituted as experimental material.
28034292	0	114	theme	Pullulan	14:21	arg1	Effect					4:9	The Effect	0:9	The Effect of Pullulan on the Growth and Acidifying Activity of Selected Stool Microflora of Human.	0:98	The Effect of Pullulan on the Growth and Acidifying Activity of Selected Stool Microflora of Human.
28034292	13	115	theme	selective	2017:2025	arg1	effect					2027:2032	selective effect	2017:2032	selective effect	2017:2032	Pullulan exhibited selective effect on the natural microflora of the colon.
28034292	13	116	theme	natural	2041:2047	arg1	microflora					2049:2058	the natural microflora	2037:2058	the natural microflora of the colon	2037:2071	Pullulan exhibited selective effect on the natural microflora of the colon.
28034292	10	117	dep	h.	1416:1417	arg1	RESULTS					1419:1425	RESULTS	1419:1425	RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH	1419:1516	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	2	118	theme	intestinal	374:383	arg1	bacteria					385:392	selected human intestinal bacteria	359:392	selected human intestinal bacteria	359:392	The objective of the study was to determine the effect of pullulan on the growth and fermentation activity of selected human intestinal bacteria.
28034292	15	119	theme	colon	2323:2327	arg1	microflora					2296:2305	the natural microflora	2284:2305	the natural microflora of the infants' colon	2284:2327	CONCLUSION In summary, pullulan exhibited a selective effect on the natural microflora of the infants' colon.
28034292	12	120	dep	obtained	1884:1891	arg1	ml					1914:1915	1.10 and 0.60 g/100 ml	1894:1915	1.10 and 0.60 g/100 ml	1894:1915	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
28034292	9	121	theme	anaerobic	1315:1323	arg1	tubes					1325:1329	anaerobic tubes	1315:1329	anaerobic tubes with AnaeroGenTM 2.5 l sachets	1315:1360	After mixing, flasks were placed in anaerobic tubes with AnaeroGenTM 2.5 l sachets.
28034292	10	122	theme	culture	1543:1549	arg1	acidity					1528:1534	total acidity	1522:1534	total acidity of the culture during 0, 24, and 48 h	1522:1572	Incubation of samples was carried out at 37°C for 48 h. RESULTS The number of Bifidobacterium, Lactobacillus, and Escherichia coli bacteria, as well as pH and total acidity of the culture during 0, 24, and 48 h were measured.
28034292	2	123	theme	selected	359:366	arg1	bacteria					385:392	selected human intestinal bacteria	359:392	selected human intestinal bacteria	359:392	The objective of the study was to determine the effect of pullulan on the growth and fermentation activity of selected human intestinal bacteria.
28034292	6	124	theme	soluble	973:979	arg1	starch					981:986	soluble starch	973:986	soluble starch	973:986	Experimental medium was the modified RCM medium, wherein the soluble starch and glucose were replaced by pullulan at a concentration of 2.0% (RCM+P).
28034292	14	125	from	activity	2103:2110	arg1	medium					2127:2132	medium	2127:2132	medium with pullulan	2127:2146	Increase in the fermentation activity of bacteria in medium with pullulan favorably influenced modification of the composition of gut microbiota.
28034292	14	126	theme	bacteria	2115:2122	arg1	activity					2103:2110	the fermentation activity	2086:2110	the fermentation activity of bacteria in medium with pullulan	2086:2146	Increase in the fermentation activity of bacteria in medium with pullulan favorably influenced modification of the composition of gut microbiota.
28034292	5	127	theme	soluble	658:664	arg1	starch					666:671	0.1 soluble starch	654:671	0.1 soluble starch	654:671	Two control media were prepared: the first standard RCM composed of (g/100 ml): 0.5 glucose, 0.1 soluble starch, 1.0 peptone, 1.0 meat extract, 0.3 yeast extract, 0.3 sodium acetate, 0.05 cysteine hydrochloride, 0.5 NaCl, pH 6.8; and the second modified RCM, wherein the soluble starch was replaced by increased glucose concentration to 2.0% (RCM+G).
28034292	1	128	theme	foods	206:210	arg1	component					181:189	component	181:189	component	181:189	BACKGROUND Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics.
28034292	12	129	theme	1.48	1810:1813	arg1	ml					1821:1822	ml	1821:1822	ml	1821:1822	Higher total acidity (1.48 g/100 ml) of pullulan culture in comparison to the control media was obtained (1.10 and 0.60 g/100 ml), and lower pH values than RCM medium, particularly 4.15 and 4.70, respectively.
24259507	6	0	from	hydrogels	998:1006	arg1	investigation					912:924	A thorough investigation	901:924	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels	901:1006	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	1	theme	alginate-based	983:996	arg1	hydrogels					998:1006	swollen alginate-based hydrogels	975:1006	swollen alginate-based hydrogels	975:1006	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	3	2	theme	patient	525:531	arg1	safety					533:538	patient safety	525:538	patient safety	525:538	To use a hydrogel in a clinical setting, terminal sterilization is necessary to ensure patient safety.
24259507	4	3	theme	negative	614:621	arg1	consequences					623:634	negative consequences	614:634	negative consequences	614:634	Lyophilization, gamma-irradiation, and ethylene oxide treatment all have negative consequences when applied to alginate scaffolds for clinical use.
24259507	0	4	theme	mechanical	69:78	arg1	properties					80:89	mechanical properties	69:89	mechanical properties	69:89	Terminal sterilization of alginate hydrogels: efficacy and impact on mechanical properties.
24259507	6	5	theme	swollen	975:981	arg1	hydrogels					998:1006	swollen alginate-based hydrogels	975:1006	swollen alginate-based hydrogels	975:1006	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	7	6	theme	structural	1221:1230	arg1	integrity					1232:1240	structural integrity	1221:1240	structural integrity	1221:1240	Preservation of structural integrity is evaluated using shear rheology and analysis of water retention, and efficacy of sterilization is determined via bacterial persistence within the hydrogel.
24259507	6	7	theme	composite	1184:1192	arg1	hydrogels					1194:1202	composite hydrogels	1184:1202	composite hydrogels	1184:1202	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	8	theme	alginate/Pluronic®	1161:1178	arg1	F68					1180:1182	alginate/Pluronic® F68	1161:1182	alginate/Pluronic® F68	1161:1182	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	7	9	theme	retention	1298:1306	arg1	rheology					1267:1274	shear rheology	1261:1274	shear rheology	1261:1274	Preservation of structural integrity is evaluated using shear rheology and analysis of water retention, and efficacy of sterilization is determined via bacterial persistence within the hydrogel.
24259507	7	9	theme	retention	1298:1306	arg1	analysis					1280:1287	analysis	1280:1287	analysis of water retention	1280:1306	Preservation of structural integrity is evaluated using shear rheology and analysis of water retention, and efficacy of sterilization is determined via bacterial persistence within the hydrogel.
24259507	9	10	theme	ethanol	1657:1663	arg1	method					1693:1698	an efficacious method	1678:1698	an efficacious method for terminally sterilizing interpenetrating networks or other composite hydrogel systems	1678:1787	Furthermore, this study suggests that ethanol treatment is an efficacious method for terminally sterilizing interpenetrating networks or other composite hydrogel systems.
24259507	9	10	theme	ethanol	1657:1663	arg1	treatment					1665:1673	ethanol treatment	1657:1673	ethanol treatment	1657:1673	Furthermore, this study suggests that ethanol treatment is an efficacious method for terminally sterilizing interpenetrating networks or other composite hydrogel systems.
24259507	9	11	theme	composite	1762:1770	arg1	systems					1781:1787	other composite hydrogel systems	1756:1787	other composite hydrogel systems	1756:1787	Furthermore, this study suggests that ethanol treatment is an efficacious method for terminally sterilizing interpenetrating networks or other composite hydrogel systems.
24259507	5	12	theme	alginate-based	770:783	arg1	hydrogels					795:803	alginate and alginate-based composite hydrogels	757:803	alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications	757:898	Here, we aim to find alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications.
24259507	6	13	theme	methods	964:970	arg1	effect					933:938	the effect	929:938	the effect of common sterilization methods on swollen alginate-based hydrogels	929:1006	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	9	14	theme	efficacious	1681:1691	arg1	method					1693:1698	an efficacious method	1678:1698	an efficacious method for terminally sterilizing interpenetrating networks or other composite hydrogel systems	1678:1787	Furthermore, this study suggests that ethanol treatment is an efficacious method for terminally sterilizing interpenetrating networks or other composite hydrogel systems.
24259507	9	14	theme	efficacious	1681:1691	arg1	treatment					1665:1673	ethanol treatment	1657:1673	ethanol treatment	1657:1673	Furthermore, this study suggests that ethanol treatment is an efficacious method for terminally sterilizing interpenetrating networks or other composite hydrogel systems.
24259507	8	15	theme	best	1451:1454	arg1	sterilization					1430:1442	ethanol sterilization	1422:1442	ethanol sterilization	1422:1442	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	8	15	theme	best	1451:1454	arg1	method					1456:1461	the best method	1447:1461	the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence	1447:1616	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	8	16	from	effects	1528:1534	arg1	properties					1550:1559	mechanical properties	1539:1559	mechanical properties	1539:1559	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	8	16	from	effects	1528:1534	arg1	retention					1571:1579	water retention	1565:1579	water retention	1565:1579	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	5	17	theme	composite	785:793	arg1	hydrogels					795:803	alginate and alginate-based composite hydrogels	757:803	alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications	757:898	Here, we aim to find alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications.
24259507	0	18	from	efficacy	46:53	arg1	properties					80:89	mechanical properties	69:89	mechanical properties	69:89	Terminal sterilization of alginate hydrogels: efficacy and impact on mechanical properties.
24259507	2	19	theme	biomaterial	288:298	arg1	formulations					300:311	traditional biomaterial formulations	276:311	traditional biomaterial formulations	276:311	Recent research has focused on optimizing traditional biomaterial formulations to create better products for commercial and academic use which incorporate hydrophobic compounds or secondary gel networks.
24259507	5	20	theme	composite	837:845	arg1	networks					856:863	composite alginate networks	837:863	composite alginate networks	837:863	Here, we aim to find alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications.
24259507	7	21	theme	water	1292:1296	arg1	retention					1298:1306	water retention	1292:1306	water retention	1292:1306	Preservation of structural integrity is evaluated using shear rheology and analysis of water retention, and efficacy of sterilization is determined via bacterial persistence within the hydrogel.
24259507	6	22	theme	common	943:948	arg1	methods					964:970	common sterilization methods	943:970	common sterilization methods	943:970	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	2	23	theme	traditional	276:286	arg1	formulations					300:311	traditional biomaterial formulations	276:311	traditional biomaterial formulations	276:311	Recent research has focused on optimizing traditional biomaterial formulations to create better products for commercial and academic use which incorporate hydrophobic compounds or secondary gel networks.
24259507	2	24	theme	gel	424:426	arg1	networks					428:435	secondary gel networks	414:435	secondary gel networks	414:435	Recent research has focused on optimizing traditional biomaterial formulations to create better products for commercial and academic use which incorporate hydrophobic compounds or secondary gel networks.
24259507	5	25	from	use	869:871	arg1	applications					887:898	biomedical applications	876:898	biomedical applications	876:898	Here, we aim to find alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications.
24259507	7	26	theme	bacterial	1357:1365	arg1	persistence					1367:1377	bacterial persistence	1357:1377	bacterial persistence within the hydrogel	1357:1397	Preservation of structural integrity is evaluated using shear rheology and analysis of water retention, and efficacy of sterilization is determined via bacterial persistence within the hydrogel.
24259507	6	27	theme	sterilization	950:962	arg1	methods					964:970	common sterilization methods	943:970	common sterilization methods	943:970	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	2	28	theme	secondary	414:422	arg1	networks					428:435	secondary gel networks	414:435	secondary gel networks	414:435	Recent research has focused on optimizing traditional biomaterial formulations to create better products for commercial and academic use which incorporate hydrophobic compounds or secondary gel networks.
24259507	0	29	theme	Terminal	0:7	arg1	sterilization					9:21	Terminal sterilization	0:21	Terminal sterilization of alginate hydrogels: efficacy and impact on mechanical properties.	0:90	Terminal sterilization of alginate hydrogels: efficacy and impact on mechanical properties.
24259507	9	30	theme	interpenetrating	1727:1742	arg1	networks					1744:1751	interpenetrating networks	1727:1751	interpenetrating networks	1727:1751	Furthermore, this study suggests that ethanol treatment is an efficacious method for terminally sterilizing interpenetrating networks or other composite hydrogel systems.
24259507	1	31	theme	Terminal	92:99	arg1	sterilization					121:133	Terminal, or postprocessing, sterilization	92:133	Terminal, or postprocessing, sterilization of composite biomaterials	92:159	Terminal, or postprocessing, sterilization of composite biomaterials is crucial for their use in wound healing and tissue-engineered devices.
24259507	0	32	theme	alginate	26:33	arg1	hydrogels					35:43	alginate hydrogels	26:43	alginate hydrogels	26:43	Terminal sterilization of alginate hydrogels: efficacy and impact on mechanical properties.
24259507	4	33	theme	ethylene	580:587	arg1	oxide					589:593	ethylene oxide	580:593	ethylene oxide	580:593	Lyophilization, gamma-irradiation, and ethylene oxide treatment all have negative consequences when applied to alginate scaffolds for clinical use.
24259507	5	34	theme	sterilization	731:743	arg1	methods					745:751	alternative terminal sterilization methods	710:751	alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications	710:898	Here, we aim to find alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications.
24259507	9	35	theme	hydrogel	1772:1779	arg1	systems					1781:1787	other composite hydrogel systems	1756:1787	other composite hydrogel systems	1756:1787	Furthermore, this study suggests that ethanol treatment is an efficacious method for terminally sterilizing interpenetrating networks or other composite hydrogel systems.
24259507	2	36	theme	better	323:328	arg1	products					330:337	better products	323:337	better products	323:337	Recent research has focused on optimizing traditional biomaterial formulations to create better products for commercial and academic use which incorporate hydrophobic compounds or secondary gel networks.
24259507	1	37	theme	wound	189:193	arg1	healing					195:201	wound healing	189:201	wound healing	189:201	Terminal, or postprocessing, sterilization of composite biomaterials is crucial for their use in wound healing and tissue-engineered devices.
24259507	5	38	theme	biomedical	876:885	arg1	applications					887:898	biomedical applications	876:898	biomedical applications	876:898	Here, we aim to find alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications.
24259507	8	39	theme	those	1466:1470	arg1	sterilization					1430:1442	ethanol sterilization	1422:1442	ethanol sterilization	1422:1442	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	8	39	theme	those	1466:1470	arg1	method					1456:1461	the best method	1447:1461	the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence	1447:1616	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	6	40	theme	effect	933:938	arg1	investigation					912:924	A thorough investigation	901:924	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels	901:1006	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	41	dep	alginate	1148:1155	arg1	hydrogels					1194:1202	composite hydrogels	1184:1202	composite hydrogels	1184:1202	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	42	from	investigation	912:924	arg1	hydrogels					998:1006	swollen alginate-based hydrogels	975:1006	swollen alginate-based hydrogels	975:1006	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	4	43	theme	treatment	595:603	arg1	all					605:607	treatment all	595:607	treatment all	595:607	Lyophilization, gamma-irradiation, and ethylene oxide treatment all have negative consequences when applied to alginate scaffolds for clinical use.
24259507	8	44	theme	water	1565:1569	arg1	retention					1571:1579	water retention	1565:1579	water retention	1565:1579	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	7	45	theme	integrity	1232:1240	arg1	Preservation					1205:1216	Preservation	1205:1216	Preservation of structural integrity	1205:1240	Preservation of structural integrity is evaluated using shear rheology and analysis of water retention, and efficacy of sterilization is determined via bacterial persistence within the hydrogel.
24259507	0	46	theme	hydrogels	35:43	arg1	sterilization					9:21	Terminal sterilization	0:21	Terminal sterilization of alginate hydrogels: efficacy and impact on mechanical properties.	0:90	Terminal sterilization of alginate hydrogels: efficacy and impact on mechanical properties.
24259507	4	47	dep	Lyophilization	541:554	arg1	all					605:607	treatment all	595:607	treatment all	595:607	Lyophilization, gamma-irradiation, and ethylene oxide treatment all have negative consequences when applied to alginate scaffolds for clinical use.
24259507	5	48	theme	alginate	757:764	arg1	hydrogels					795:803	alginate and alginate-based composite hydrogels	757:803	alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications	757:898	Here, we aim to find alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications.
24259507	8	49	theme	ethanol	1422:1428	arg1	sterilization					1430:1442	ethanol sterilization	1422:1442	ethanol sterilization	1422:1442	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	8	49	theme	ethanol	1422:1428	arg1	method					1456:1461	the best method	1447:1461	the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence	1447:1616	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	2	50	theme	Recent	234:239	arg1	research					241:248	Recent research	234:248	Recent research	234:248	Recent research has focused on optimizing traditional biomaterial formulations to create better products for commercial and academic use which incorporate hydrophobic compounds or secondary gel networks.
24259507	1	51	theme	tissue-engineered	207:223	arg1	devices					225:231	tissue-engineered devices	207:231	tissue-engineered devices	207:231	Terminal, or postprocessing, sterilization of composite biomaterials is crucial for their use in wound healing and tissue-engineered devices.
24259507	5	52	theme	alginate	847:854	arg1	networks					856:863	composite alginate networks	837:863	composite alginate networks	837:863	Here, we aim to find alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications.
24259507	6	53	from	effect	933:938	arg1	hydrogels					998:1006	swollen alginate-based hydrogels	975:1006	swollen alginate-based hydrogels	975:1006	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	0	54	from	impact	59:64	arg1	properties					80:89	mechanical properties	69:89	mechanical properties	69:89	Terminal sterilization of alginate hydrogels: efficacy and impact on mechanical properties.
24259507	4	55	contain	have	609:612	arg1	oxide					589:593	ethylene oxide	580:593	ethylene oxide	580:593	Lyophilization, gamma-irradiation, and ethylene oxide treatment all have negative consequences when applied to alginate scaffolds for clinical use.
24259507	4	55	contain	have	609:612	arg2	consequences					623:634	negative consequences	614:634	negative consequences	614:634	Lyophilization, gamma-irradiation, and ethylene oxide treatment all have negative consequences when applied to alginate scaffolds for clinical use.
24259507	4	55	contain	have	609:612	arg1	gamma-irradiation					557:573	gamma-irradiation	557:573	gamma-irradiation	557:573	Lyophilization, gamma-irradiation, and ethylene oxide treatment all have negative consequences when applied to alginate scaffolds for clinical use.
24259507	4	55	contain	have	609:612	arg1	Lyophilization					541:554	Lyophilization	541:554	Lyophilization	541:554	Lyophilization, gamma-irradiation, and ethylene oxide treatment all have negative consequences when applied to alginate scaffolds for clinical use.
24259507	1	56	theme	postprocessing	105:118	arg1	sterilization					121:133	Terminal, or postprocessing, sterilization	92:133	Terminal, or postprocessing, sterilization of composite biomaterials	92:159	Terminal, or postprocessing, sterilization of composite biomaterials is crucial for their use in wound healing and tissue-engineered devices.
24259507	5	57	theme	networks	856:863	arg1	structure					824:832	the structure	820:832	the structure of composite alginate networks for use in biomedical applications	820:898	Here, we aim to find alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications.
24259507	6	58	theme	thorough	903:910	arg1	investigation					912:924	A thorough investigation	901:924	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels	901:1006	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	8	59	theme	minimal	1520:1526	arg1	effects					1528:1534	minimal effects	1520:1534	minimal effects on mechanical properties and water retention	1520:1579	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	2	60	theme	academic	358:365	arg1	use					367:369	academic use	358:369	academic use	358:369	Recent research has focused on optimizing traditional biomaterial formulations to create better products for commercial and academic use which incorporate hydrophobic compounds or secondary gel networks.
24259507	7	61	theme	shear	1261:1265	arg1	rheology					1267:1274	shear rheology	1261:1274	shear rheology	1261:1274	Preservation of structural integrity is evaluated using shear rheology and analysis of water retention, and efficacy of sterilization is determined via bacterial persistence within the hydrogel.
24259507	5	62	theme	alternative	710:720	arg1	methods					745:751	alternative terminal sterilization methods	710:751	alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications	710:898	Here, we aim to find alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications.
24259507	8	63	theme	bacterial	1596:1604	arg1	persistence					1606:1616	bacterial persistence	1596:1616	bacterial persistence	1596:1616	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	8	64	theme	mechanical	1539:1548	arg1	properties					1550:1559	mechanical properties	1539:1559	mechanical properties	1539:1559	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	9	65	theme	other	1756:1760	arg1	systems					1781:1787	other composite hydrogel systems	1756:1787	other composite hydrogel systems	1756:1787	Furthermore, this study suggests that ethanol treatment is an efficacious method for terminally sterilizing interpenetrating networks or other composite hydrogel systems.
24259507	8	66	theme	washing	1501:1507	arg1	ethanol					1493:1499	ethanol washing	1493:1507	ethanol washing	1493:1507	Results indicate that ethanol sterilization is the best method of those investigated because ethanol washing results in minimal effects on mechanical properties and water retention and eliminates bacterial persistence.
24259507	5	67	theme	terminal	722:729	arg1	methods					745:751	alternative terminal sterilization methods	710:751	alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications	710:898	Here, we aim to find alternative terminal sterilization methods for alginate and alginate-based composite hydrogels which maintain the structure of composite alginate networks for use in biomedical applications.
24259507	6	68	theme	sterilization	1119:1131	arg1	autoclaving					1064:1074	autoclaving	1064:1074	autoclaving	1064:1074	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	68	theme	sterilization	1119:1131	arg1	light					1110:1114	ultraviolet light	1098:1114	ultraviolet light	1098:1114	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	68	theme	sterilization	1119:1131	arg1	techniques					1133:1142	sterilization techniques	1119:1142	sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels	1119:1202	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	68	theme	sterilization	1119:1131	arg1	ethanol					1077:1083	ethanol washing	1077:1091	ethanol washing	1077:1091	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	1	69	from	use	182:184	arg1	healing					195:201	wound healing	189:201	wound healing	189:201	Terminal, or postprocessing, sterilization of composite biomaterials is crucial for their use in wound healing and tissue-engineered devices.
24259507	1	69	from	use	182:184	arg1	devices					225:231	tissue-engineered devices	207:231	tissue-engineered devices	207:231	Terminal, or postprocessing, sterilization of composite biomaterials is crucial for their use in wound healing and tissue-engineered devices.
24259507	2	70	theme	hydrophobic	389:399	arg1	compounds					401:409	hydrophobic compounds	389:409	hydrophobic compounds	389:409	Recent research has focused on optimizing traditional biomaterial formulations to create better products for commercial and academic use which incorporate hydrophobic compounds or secondary gel networks.
24259507	6	71	theme	ultraviolet	1098:1108	arg1	autoclaving					1064:1074	autoclaving	1064:1074	autoclaving	1064:1074	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	71	theme	ultraviolet	1098:1108	arg1	light					1110:1114	ultraviolet light	1098:1114	ultraviolet light	1098:1114	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	71	theme	ultraviolet	1098:1108	arg1	techniques					1133:1142	sterilization techniques	1119:1142	sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels	1119:1202	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	71	theme	ultraviolet	1098:1108	arg1	ethanol					1077:1083	ethanol washing	1077:1091	ethanol washing	1077:1091	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	0	72	dep	sterilization	9:21	arg1	impact					59:64	impact	59:64	impact	59:64	Terminal sterilization of alginate hydrogels: efficacy and impact on mechanical properties.
24259507	0	72	dep	sterilization	9:21	arg1	efficacy					46:53	efficacy	46:53	efficacy	46:53	Terminal sterilization of alginate hydrogels: efficacy and impact on mechanical properties.
24259507	3	73	theme	terminal	479:486	arg1	sterilization					488:500	terminal sterilization	479:500	terminal sterilization	479:500	To use a hydrogel in a clinical setting, terminal sterilization is necessary to ensure patient safety.
24259507	1	74	theme	composite	138:146	arg1	biomaterials					148:159	composite biomaterials	138:159	composite biomaterials	138:159	Terminal, or postprocessing, sterilization of composite biomaterials is crucial for their use in wound healing and tissue-engineered devices.
24259507	3	75	theme	clinical	461:468	arg1	setting					470:476	a clinical setting	459:476	a clinical setting	459:476	To use a hydrogel in a clinical setting, terminal sterilization is necessary to ensure patient safety.
24259507	6	76	theme	washing	1085:1091	arg1	autoclaving					1064:1074	autoclaving	1064:1074	autoclaving	1064:1074	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	76	theme	washing	1085:1091	arg1	techniques					1133:1142	sterilization techniques	1119:1142	sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels	1119:1202	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	76	theme	washing	1085:1091	arg1	ethanol					1077:1083	ethanol washing	1077:1091	ethanol washing	1077:1091	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	6	76	theme	washing	1085:1091	arg1	light					1110:1114	ultraviolet light	1098:1114	ultraviolet light	1098:1114	A thorough investigation of the effect of common sterilization methods on swollen alginate-based hydrogels has not been reported and therefore, this work examines autoclaving, ethanol washing, and ultraviolet light as sterilization techniques for alginate and alginate/Pluronic® F68 composite hydrogels.
24259507	7	77	theme	sterilization	1325:1337	arg1	efficacy					1313:1320	efficacy	1313:1320	efficacy of sterilization	1313:1337	Preservation of structural integrity is evaluated using shear rheology and analysis of water retention, and efficacy of sterilization is determined via bacterial persistence within the hydrogel.
24259507	4	78	theme	clinical	675:682	arg1	use					684:686	clinical use	675:686	clinical use	675:686	Lyophilization, gamma-irradiation, and ethylene oxide treatment all have negative consequences when applied to alginate scaffolds for clinical use.
24259507	1	79	theme	biomaterials	148:159	arg1	sterilization					121:133	Terminal, or postprocessing, sterilization	92:133	Terminal, or postprocessing, sterilization of composite biomaterials	92:159	Terminal, or postprocessing, sterilization of composite biomaterials is crucial for their use in wound healing and tissue-engineered devices.
24259507	4	80	theme	alginate	652:659	arg1	scaffolds					661:669	alginate scaffolds	652:669	alginate scaffolds for clinical use	652:686	Lyophilization, gamma-irradiation, and ethylene oxide treatment all have negative consequences when applied to alginate scaffolds for clinical use.
28521997	4	0	theme	water	579:583	arg1	surface					618:624	the positive surface	605:624	the positive surface rich in amino groups and high specific surface area (952.5m2g-1)	605:689	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	0	theme	water	579:583	arg1	ability					596:602	rapid water absorption ability	573:602	rapid water absorption ability	573:602	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	1	theme	specific	656:663	arg1	952.5m2g-1					679:688	952.5m2g-1	679:688	952.5m2g-1	679:688	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	1	theme	specific	656:663	arg1	area					673:676	high specific surface area	651:676	high specific surface area (952.5m2g-1)	651:689	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	2	from	rich	626:629	arg1	952.5m2g-1					679:688	952.5m2g-1	679:688	952.5m2g-1	679:688	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	2	from	rich	626:629	arg1	area					673:676	high specific surface area	651:676	high specific surface area (952.5m2g-1)	651:689	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	2	from	rich	626:629	arg1	groups					640:645	amino groups	634:645	amino groups	634:645	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	3	theme	intrinsic	768:776	arg1	pathway					778:784	the intrinsic pathway	764:784	the intrinsic pathway	764:784	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	4	theme	high	651:654	arg1	952.5m2g-1					679:688	952.5m2g-1	679:688	952.5m2g-1	679:688	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	4	theme	high	651:654	arg1	area					673:676	high specific surface area	651:676	high specific surface area (952.5m2g-1)	651:689	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	3	5	theme	collagen	421:428	arg1	sponge					430:435	a biodegradable collagen sponge	405:435	a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis	405:534	In facing these challenges, we designed a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis.
28521997	5	6	theme	peritoneal	1060:1069	arg1	prevention					1080:1089	peritoneal adhesion prevention	1060:1089	peritoneal adhesion prevention	1060:1089	In addition, the CNPFs-Col sponge can be completely biodegraded in 3 weeks, which is suitable for post-operative treatment and peritoneal adhesion prevention.
28521997	1	7	from	military	206:213	arg1	important					180:188	important	180:188	important	180:188	Efficient and biodegradable hemostatic materials become increasingly important in civilian and military clinical.
28521997	1	8	dep	civilian	193:200	arg1	clinical					215:222	clinical	215:222	clinical	215:222	Efficient and biodegradable hemostatic materials become increasingly important in civilian and military clinical.
28521997	4	9	theme	haemocytes	817:826	arg1	adherence					842:850	haemocytes and platelets adherence	817:850	haemocytes and platelets adherence	817:850	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	2	10	theme	rich	347:350	arg1	vascularity					352:362	rich vascularity	347:362	rich vascularity	347:362	However, traditional hemostatic materials are difficult to achieve expected effects especially in parenchymal organs with rich vascularity.
28521997	4	11	theme	surface	665:671	arg1	952.5m2g-1					679:688	952.5m2g-1	679:688	952.5m2g-1	679:688	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	11	theme	surface	665:671	arg1	area					673:676	high specific surface area	651:676	high specific surface area (952.5m2g-1)	651:689	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	1	12	from	civilian	193:200	arg1	important					180:188	important	180:188	important	180:188	Efficient and biodegradable hemostatic materials become increasingly important in civilian and military clinical.
28521997	5	13	theme	post-operative	1031:1044	arg1	treatment					1046:1054	post-operative treatment	1031:1054	post-operative treatment	1031:1054	In addition, the CNPFs-Col sponge can be completely biodegraded in 3 weeks, which is suitable for post-operative treatment and peritoneal adhesion prevention.
28521997	2	14	from	difficult	271:279	arg1	organs					335:340	parenchymal organs	323:340	parenchymal organs with rich vascularity	323:362	However, traditional hemostatic materials are difficult to achieve expected effects especially in parenchymal organs with rich vascularity.
28521997	3	15	theme	rapid	519:523	arg1	hemostasis					525:534	rapid hemostasis	519:534	rapid hemostasis	519:534	In facing these challenges, we designed a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis.
28521997	3	16	theme	biodegradable	407:419	arg1	sponge					430:435	a biodegradable collagen sponge	405:435	a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis	405:534	In facing these challenges, we designed a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis.
28521997	4	17	theme	rapid	573:577	arg1	surface					618:624	the positive surface	605:624	the positive surface rich in amino groups and high specific surface area (952.5m2g-1)	605:689	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	17	theme	rapid	573:577	arg1	ability					596:602	rapid water absorption ability	573:602	rapid water absorption ability	573:602	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	3	18	theme	CPNFs-Col	497:505	arg1	nanoflowers					484:494	chitosan/calcium pyrophosphate nanoflowers	453:494	chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge)	453:513	In facing these challenges, we designed a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis.
28521997	3	18	theme	CPNFs-Col	497:505	arg1	sponge					507:512	CPNFs-Col sponge	497:512	CPNFs-Col sponge	497:512	In facing these challenges, we designed a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis.
28521997	3	19	theme	pyrophosphate	470:482	arg1	nanoflowers					484:494	chitosan/calcium pyrophosphate nanoflowers	453:494	chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge)	453:513	In facing these challenges, we designed a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis.
28521997	3	19	theme	pyrophosphate	470:482	arg1	sponge					507:512	CPNFs-Col sponge	497:512	CPNFs-Col sponge	497:512	In facing these challenges, we designed a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis.
28521997	6	20	theme	promising	1153:1161	arg1	candidate					1163:1171	a promising candidate	1151:1171	a promising candidate for clinical hemostatic applications	1151:1208	It can be concluded that the CPNFs-Col sponge would become a promising candidate for clinical hemostatic applications.
28521997	3	21	theme	chitosan/calcium	453:468	arg1	nanoflowers					484:494	chitosan/calcium pyrophosphate nanoflowers	453:494	chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge)	453:513	In facing these challenges, we designed a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis.
28521997	3	21	theme	chitosan/calcium	453:468	arg1	sponge					507:512	CPNFs-Col sponge	497:512	CPNFs-Col sponge	497:512	In facing these challenges, we designed a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis.
28521997	4	22	theme	CNPFs-Col	705:713	arg1	sponge					715:720	the obtained CNPFs-Col sponge	692:720	the obtained CNPFs-Col sponge with optimized composition	692:747	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	1	23	from	important	180:188	arg1	civilian					193:200	civilian	193:200	civilian	193:200	Efficient and biodegradable hemostatic materials become increasingly important in civilian and military clinical.
28521997	1	23	from	important	180:188	arg1	military					206:213	military	206:213	military	206:213	Efficient and biodegradable hemostatic materials become increasingly important in civilian and military clinical.
28521997	1	24	theme	Efficient	111:119	arg1	materials					150:158	Efficient and biodegradable hemostatic materials	111:158	Efficient and biodegradable hemostatic materials	111:158	Efficient and biodegradable hemostatic materials become increasingly important in civilian and military clinical.
28521997	0	25	theme	Biodegradable	0:12	arg1	sponge					23:28	Biodegradable collagen sponge	0:28	Biodegradable collagen sponge	0:28	Biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers for rapid hemostasis.
28521997	4	26	theme	amino	634:638	arg1	groups					640:645	amino groups	634:645	amino groups	634:645	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	27	from	groups	640:645	arg1	rich					626:629	rich	626:629	rich	626:629	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	1	28	theme	biodegradable	125:137	arg1	materials					150:158	Efficient and biodegradable hemostatic materials	111:158	Efficient and biodegradable hemostatic materials	111:158	Efficient and biodegradable hemostatic materials become increasingly important in civilian and military clinical.
28521997	4	29	theme	obtained	696:703	arg1	sponge					715:720	the obtained CNPFs-Col sponge	692:720	the obtained CNPFs-Col sponge with optimized composition	692:747	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	1	30	theme	hemostatic	139:148	arg1	materials					150:158	Efficient and biodegradable hemostatic materials	111:158	Efficient and biodegradable hemostatic materials	111:158	Efficient and biodegradable hemostatic materials become increasingly important in civilian and military clinical.
28521997	6	31	theme	clinical	1177:1184	arg1	applications					1197:1208	clinical hemostatic applications	1177:1208	clinical hemostatic applications	1177:1208	It can be concluded that the CPNFs-Col sponge would become a promising candidate for clinical hemostatic applications.
28521997	0	32	theme	chitosan/calcium	46:61	arg1	nanoflowers					77:87	chitosan/calcium pyrophosphate nanoflowers	46:87	chitosan/calcium pyrophosphate nanoflowers for rapid hemostasis	46:108	Biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers for rapid hemostasis.
28521997	2	33	theme	expected	292:299	arg1	effects					301:307	expected effects	292:307	expected effects	292:307	However, traditional hemostatic materials are difficult to achieve expected effects especially in parenchymal organs with rich vascularity.
28521997	4	34	theme	optimized	727:735	arg1	composition					737:747	optimized composition	727:747	optimized composition	727:747	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	35	theme	positive	609:616	arg1	surface					618:624	the positive surface	605:624	the positive surface rich in amino groups and high specific surface area (952.5m2g-1)	605:689	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	35	theme	positive	609:616	arg1	ability					596:602	rapid water absorption ability	573:602	rapid water absorption ability	573:602	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	36	theme	hemorrhage	892:901	arg1	control					903:909	hemorrhage control	892:909	hemorrhage control	892:909	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	37	theme	platelets	832:840	arg1	adherence					842:850	haemocytes and platelets adherence	817:850	haemocytes and platelets adherence	817:850	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	38	from	area	673:676	arg1	rich					626:629	rich	626:629	rich	626:629	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	39	theme	specific	542:549	arg1	ability					596:602	rapid water absorption ability	573:602	rapid water absorption ability	573:602	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	39	theme	specific	542:549	arg1	performances					551:562	specific performances	542:562	specific performances	542:562	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	40	theme	coagulation	789:799	arg1	cascade					801:807	coagulation cascade	789:807	coagulation cascade	789:807	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	0	41	theme	pyrophosphate	63:75	arg1	nanoflowers					77:87	chitosan/calcium pyrophosphate nanoflowers	46:87	chitosan/calcium pyrophosphate nanoflowers for rapid hemostasis	46:108	Biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers for rapid hemostasis.
28521997	4	42	theme	rich	626:629	arg1	surface					618:624	the positive surface	605:624	the positive surface rich in amino groups and high specific surface area (952.5m2g-1)	605:689	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	42	theme	rich	626:629	arg1	ability					596:602	rapid water absorption ability	573:602	rapid water absorption ability	573:602	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	43	with	sponge	715:720	arg1	composition					737:747	optimized composition	727:747	optimized composition	727:747	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	2	44	theme	parenchymal	323:333	arg1	organs					335:340	parenchymal organs	323:340	parenchymal organs with rich vascularity	323:362	However, traditional hemostatic materials are difficult to achieve expected effects especially in parenchymal organs with rich vascularity.
28521997	2	45	from	organs	335:340	arg1	difficult					271:279	difficult	271:279	difficult	271:279	However, traditional hemostatic materials are difficult to achieve expected effects especially in parenchymal organs with rich vascularity.
28521997	4	46	dep	activate	755:762	arg1	induce					810:815	induce	810:815	induce haemocytes and platelets adherence	810:850	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	46	dep	activate	755:762	arg1	achieve					884:890	achieve	884:890	achieve hemorrhage control in vitro	884:918	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	46	dep	activate	755:762	arg1	promote					853:859	promote	853:859	promote the blood clotting	853:878	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	2	47	theme	hemostatic	246:255	arg1	materials					257:265	traditional hemostatic materials	234:265	traditional hemostatic materials	234:265	However, traditional hemostatic materials are difficult to achieve expected effects especially in parenchymal organs with rich vascularity.
28521997	4	48	theme	blood	865:869	arg1	clotting					871:878	the blood clotting	861:878	the blood clotting	861:878	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	6	49	theme	hemostatic	1186:1195	arg1	applications					1197:1208	clinical hemostatic applications	1177:1208	clinical hemostatic applications	1177:1208	It can be concluded that the CPNFs-Col sponge would become a promising candidate for clinical hemostatic applications.
28521997	2	50	with	organs	335:340	arg1	vascularity					352:362	rich vascularity	347:362	rich vascularity	347:362	However, traditional hemostatic materials are difficult to achieve expected effects especially in parenchymal organs with rich vascularity.
28521997	0	51	theme	rapid	93:97	arg1	hemostasis					99:108	rapid hemostasis	93:108	rapid hemostasis	93:108	Biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers for rapid hemostasis.
28521997	2	52	theme	traditional	234:244	arg1	materials					257:265	traditional hemostatic materials	234:265	traditional hemostatic materials	234:265	However, traditional hemostatic materials are difficult to achieve expected effects especially in parenchymal organs with rich vascularity.
28521997	5	53	theme	adhesion	1071:1078	arg1	prevention					1080:1089	peritoneal adhesion prevention	1060:1089	peritoneal adhesion prevention	1060:1089	In addition, the CNPFs-Col sponge can be completely biodegraded in 3 weeks, which is suitable for post-operative treatment and peritoneal adhesion prevention.
28521997	6	54	theme	CPNFs-Col	1121:1129	arg1	sponge					1131:1136	the CPNFs-Col sponge	1117:1136	the CPNFs-Col sponge	1117:1136	It can be concluded that the CPNFs-Col sponge would become a promising candidate for clinical hemostatic applications.
28521997	5	55	theme	CNPFs-Col	950:958	arg1	sponge					960:965	the CNPFs-Col sponge	946:965	the CNPFs-Col sponge	946:965	In addition, the CNPFs-Col sponge can be completely biodegraded in 3 weeks, which is suitable for post-operative treatment and peritoneal adhesion prevention.
28521997	4	56	theme	absorption	585:594	arg1	surface					618:624	the positive surface	605:624	the positive surface rich in amino groups and high specific surface area (952.5m2g-1)	605:689	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	4	56	theme	absorption	585:594	arg1	ability					596:602	rapid water absorption ability	573:602	rapid water absorption ability	573:602	With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5m2g-1), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo.
28521997	0	57	theme	collagen	14:21	arg1	sponge					23:28	Biodegradable collagen sponge	0:28	Biodegradable collagen sponge	0:28	Biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers for rapid hemostasis.
27773574	0	0	theme	ACI	81:83	arg1	tissue					86:91	autologous chondrocyte implantation (ACI) tissue	44:91	autologous chondrocyte implantation (ACI) tissue	44:91	A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures.
27773574	5	1	theme	20months	739:746	arg1	post-ACI-treatment					748:765	20months post-ACI-treatment	739:765	20months post-ACI-treatment	739:765	METHODS Biopsies were taken from one patient (25years old) at 12months and 20months post-ACI-treatment and from three normal cadavers (21, 22 and 25years old).
27773574	5	2	dep	old	718:720	arg1	25years					710:716	25years	710:716	25years	710:716	METHODS Biopsies were taken from one patient (25years old) at 12months and 20months post-ACI-treatment and from three normal cadavers (21, 22 and 25years old).
27773574	1	3	theme	chondrocyte	146:156	arg1	ACI					172:174	ACI	172:174	ACI	172:174	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	1	3	theme	chondrocyte	146:156	arg1	implantation					158:169	BACKGROUND Autologous chondrocyte implantation	124:169	BACKGROUND Autologous chondrocyte implantation (ACI)	124:175	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	7	4	theme	age-matched	1018:1028	arg1	biopsies					1040:1047	the age-matched cadaveric biopsies	1014:1047	the age-matched cadaveric biopsies	1014:1047	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	8	5	theme	chondroitin	1177:1187	arg1	sulphate					1189:1196	chondroitin sulphate	1177:1196	chondroitin sulphate disaccharides	1177:1210	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	7	6	theme	same	1088:1091	arg1	hyaluronan					1103:1112	hyaluronan	1103:1112	hyaluronan	1103:1112	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	7	6	theme	same	1088:1091	arg1	controls					1121:1128	the controls	1117:1128	the controls	1117:1128	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	7	6	theme	same	1088:1091	arg1	amount					1093:1098	the same amount	1084:1098	the same amount of hyaluronan	1084:1112	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	8	7	theme	sulphate	1189:1196	arg1	disaccharides					1198:1210	chondroitin sulphate disaccharides	1177:1210	chondroitin sulphate disaccharides	1177:1210	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	1	8	theme	patients	234:241	arg1	thousands					221:229	thousands	221:229	thousands of patients worldwide with good clinical effectiveness 10-20years after implantation	221:314	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	0	9	theme	tissue	86:91	arg1	profiles					32:39	Glycosaminoglycan profiles	14:39	Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue	14:91	A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures.
27773574	5	10	dep	cadavers	789:796	arg1	old					818:820	old	818:820	old	818:820	METHODS Biopsies were taken from one patient (25years old) at 12months and 20months post-ACI-treatment and from three normal cadavers (21, 22 and 25years old).
27773574	10	11	theme	tissue	1562:1567	arg1	identical					1576:1584	identical	1576:1584	identical	1576:1584	CONCLUSIONS Although the glycosaminoglycan composition of the repair tissue is not identical to mature articular cartilage its quality continues to improve with time.
27773574	10	11	theme	tissue	1562:1567	arg1	composition					1536:1546	the glycosaminoglycan composition	1514:1546	the glycosaminoglycan composition of the repair tissue	1514:1567	CONCLUSIONS Although the glycosaminoglycan composition of the repair tissue is not identical to mature articular cartilage its quality continues to improve with time.
27773574	7	12	theme	ACI	974:976	arg1	biopsy					978:983	the ACI biopsy	970:983	12months the ACI biopsy	961:983	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	1	13	theme	worldwide	243:251	arg1	patients					234:241	patients	234:241	patients worldwide with good clinical effectiveness 10-20years after implantation	234:314	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	6	14	used	used	885:888	arg2	electrophoresis					858:872	Fluorophore-assisted carbohydrate electrophoresis	824:872	Fluorophore-assisted carbohydrate electrophoresis (FACE)	824:879	Fluorophore-assisted carbohydrate electrophoresis (FACE) was used to quantitatively assess the individual glycosaminoglycans.
27773574	6	14	used	used	885:888	arg2	FACE					875:878	FACE	875:878	FACE	875:878	Fluorophore-assisted carbohydrate electrophoresis (FACE) was used to quantitatively assess the individual glycosaminoglycans.
27773574	3	15	theme	repair	556:561	arg1	tissue					563:568	the repair tissue	552:568	the repair tissue	552:568	Glycosaminoglycan structure influences physiological function and is likely to be important in the long term stability of the repair tissue.
27773574	10	16	theme	articular	1596:1604	arg1	cartilage					1606:1614	mature articular cartilage its quality	1589:1626	mature articular cartilage its quality	1589:1626	CONCLUSIONS Although the glycosaminoglycan composition of the repair tissue is not identical to mature articular cartilage its quality continues to improve with time.
27773574	2	17	theme	repair	359:364	arg1	cartilage					366:374	the repair cartilage	355:374	the repair cartilage	355:374	Information concerning the quality of the repair cartilage is still limited because biopsies are small and rare.
27773574	4	18	theme	study	587:591	arg1	aim					575:577	The aim	571:577	The aim of this study	571:591	The aim of this study was to assess glycosaminoglycans in ACI tissue over a two year period.
27773574	8	19	theme	12	1140:1141	arg1	biopsies					1159:1166	Both the 12 and 20month ACI biopsies	1131:1166	biopsies	1159:1166	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	4	20	theme	ACI	629:631	arg1	tissue					633:638	ACI tissue	629:638	ACI tissue	629:638	The aim of this study was to assess glycosaminoglycans in ACI tissue over a two year period.
27773574	10	21	theme	glycosaminoglycan	1518:1534	arg1	identical					1576:1584	identical	1576:1584	identical	1576:1584	CONCLUSIONS Although the glycosaminoglycan composition of the repair tissue is not identical to mature articular cartilage its quality continues to improve with time.
27773574	10	21	theme	glycosaminoglycan	1518:1534	arg1	composition					1536:1546	the glycosaminoglycan composition	1514:1546	the glycosaminoglycan composition of the repair tissue	1514:1567	CONCLUSIONS Although the glycosaminoglycan composition of the repair tissue is not identical to mature articular cartilage its quality continues to improve with time.
27773574	4	22	theme	year	651:654	arg1	period					656:661	a two year period	645:661	a two year period	645:661	The aim of this study was to assess glycosaminoglycans in ACI tissue over a two year period.
27773574	7	23	theme	hyaluronan	1103:1112	arg1	hyaluronan					1103:1112	hyaluronan	1103:1112	hyaluronan	1103:1112	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	7	23	theme	hyaluronan	1103:1112	arg1	controls					1121:1128	the controls	1117:1128	the controls	1117:1128	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	7	23	theme	hyaluronan	1103:1112	arg1	amount					1093:1098	the same amount	1084:1098	the same amount of hyaluronan	1084:1112	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	7	24	theme	less	993:996	arg1	hyaluronan					998:1007	40% less hyaluronan	989:1007	40% less hyaluronan	989:1007	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	4	25	theme	two	647:649	arg1	year					651:654	year	651:654	year	651:654	The aim of this study was to assess glycosaminoglycans in ACI tissue over a two year period.
27773574	0	26	theme	case	2:5	arg1	study					7:11	A case study	0:11	A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures.	0:122	A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures.
27773574	3	27	theme	term	534:537	arg1	stability					539:547	the long term stability	525:547	the long term stability of the repair tissue	525:568	Glycosaminoglycan structure influences physiological function and is likely to be important in the long term stability of the repair tissue.
27773574	10	28	theme	repair	1555:1560	arg1	tissue					1562:1567	the repair tissue	1551:1567	the repair tissue	1551:1567	CONCLUSIONS Although the glycosaminoglycan composition of the repair tissue is not identical to mature articular cartilage its quality continues to improve with time.
27773574	5	29	dep	patient	701:707	arg1	old					718:720	old	718:720	old	718:720	METHODS Biopsies were taken from one patient (25years old) at 12months and 20months post-ACI-treatment and from three normal cadavers (21, 22 and 25years old).
27773574	8	30	theme	chondroitin	1224:1234	arg1	chains					1245:1250	shorter chondroitin sulphate chains	1216:1250	shorter chondroitin sulphate chains	1216:1250	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	9	31	theme	sulphate	1314:1321	arg1	length					1329:1334	chondroitin sulphate chain length	1302:1334	chondroitin sulphate chain length	1302:1334	However, chondroitin sulphate chain length doubled as the ACI repair tissue matured at 12months (3913Da±464) and 20months (6923Da±711) and there was less keratan sulphate as compared to the controls.
27773574	1	32	theme	good	258:261	arg1	10-20years					286:295	good clinical effectiveness 10-20years	258:295	good clinical effectiveness 10-20years after implantation	258:314	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	7	33	theme	ACI	1069:1071	arg1	biopsy					1073:1078	the ACI biopsy	1065:1078	the ACI biopsy	1065:1078	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	8	34	theme	sulphate	1236:1243	arg1	chains					1245:1250	shorter chondroitin sulphate chains	1216:1250	shorter chondroitin sulphate chains	1216:1250	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	3	35	theme	Glycosaminoglycan	430:446	arg1	structure					448:456	Glycosaminoglycan structure	430:456	Glycosaminoglycan structure	430:456	Glycosaminoglycan structure influences physiological function and is likely to be important in the long term stability of the repair tissue.
27773574	1	36	theme	clinical	263:270	arg1	10-20years					286:295	good clinical effectiveness 10-20years	258:295	good clinical effectiveness 10-20years after implantation	258:314	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	0	37	theme	tissue	108:113	arg1	matures					115:121	the tissue matures	104:121	the tissue matures	104:121	A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures.
27773574	3	38	from	important	512:520	arg1	stability					539:547	the long term stability	525:547	the long term stability of the repair tissue	525:568	Glycosaminoglycan structure influences physiological function and is likely to be important in the long term stability of the repair tissue.
27773574	3	39	theme	tissue	563:568	arg1	stability					539:547	the long term stability	525:547	the long term stability of the repair tissue	525:568	Glycosaminoglycan structure influences physiological function and is likely to be important in the long term stability of the repair tissue.
27773574	8	40	theme	ACI	1155:1157	arg1	biopsies					1159:1166	Both the 12 and 20month ACI biopsies	1131:1166	biopsies	1159:1166	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	1	41	theme	effectiveness	272:284	arg1	10-20years					286:295	good clinical effectiveness 10-20years	258:295	good clinical effectiveness 10-20years after implantation	258:314	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	9	42	theme	chain	1323:1327	arg1	length					1329:1334	chondroitin sulphate chain length	1302:1334	chondroitin sulphate chain length	1302:1334	However, chondroitin sulphate chain length doubled as the ACI repair tissue matured at 12months (3913Da±464) and 20months (6923Da±711) and there was less keratan sulphate as compared to the controls.
27773574	6	43	theme	carbohydrate	845:856	arg1	FACE					875:878	FACE	875:878	FACE	875:878	Fluorophore-assisted carbohydrate electrophoresis (FACE) was used to quantitatively assess the individual glycosaminoglycans.
27773574	6	43	theme	carbohydrate	845:856	arg1	electrophoresis					858:872	Fluorophore-assisted carbohydrate electrophoresis	824:872	Fluorophore-assisted carbohydrate electrophoresis (FACE)	824:879	Fluorophore-assisted carbohydrate electrophoresis (FACE) was used to quantitatively assess the individual glycosaminoglycans.
27773574	7	44	from	12months	961:968	arg1	RESULTS					950:956	RESULTS	950:956	RESULTS At 12months the ACI biopsy	950:983	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	3	45	theme	long	529:532	arg1	stability					539:547	the long term stability	525:547	the long term stability of the repair tissue	525:568	Glycosaminoglycan structure influences physiological function and is likely to be important in the long term stability of the repair tissue.
27773574	8	46	dep	less	1172:1175	arg1	chains					1245:1250	shorter chondroitin sulphate chains	1216:1250	shorter chondroitin sulphate chains	1216:1250	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	8	46	dep	less	1172:1175	arg1	disaccharides					1198:1210	chondroitin sulphate disaccharides	1177:1210	chondroitin sulphate disaccharides	1177:1210	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	6	47	theme	Fluorophore-assisted	824:843	arg1	FACE					875:878	FACE	875:878	FACE	875:878	Fluorophore-assisted carbohydrate electrophoresis (FACE) was used to quantitatively assess the individual glycosaminoglycans.
27773574	6	47	theme	Fluorophore-assisted	824:843	arg1	electrophoresis					858:872	Fluorophore-assisted carbohydrate electrophoresis	824:872	Fluorophore-assisted carbohydrate electrophoresis (FACE)	824:879	Fluorophore-assisted carbohydrate electrophoresis (FACE) was used to quantitatively assess the individual glycosaminoglycans.
27773574	8	48	theme	shorter	1216:1222	arg1	chains					1245:1250	shorter chondroitin sulphate chains	1216:1250	shorter chondroitin sulphate chains	1216:1250	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	3	49	theme	physiological	469:481	arg1	function					483:490	physiological function	469:490	physiological function	469:490	Glycosaminoglycan structure influences physiological function and is likely to be important in the long term stability of the repair tissue.
27773574	0	50	theme	Glycosaminoglycan	14:30	arg1	profiles					32:39	Glycosaminoglycan profiles	14:39	Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue	14:91	A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures.
27773574	1	51	with	worldwide	243:251	arg1	10-20years					286:295	good clinical effectiveness 10-20years	258:295	good clinical effectiveness 10-20years after implantation	258:314	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	8	52	theme	20month	1147:1153	arg1	biopsies					1159:1166	Both the 12 and 20month ACI biopsies	1131:1166	biopsies	1159:1166	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	0	53	theme	autologous	44:53	arg1	tissue					86:91	autologous chondrocyte implantation (ACI) tissue	44:91	autologous chondrocyte implantation (ACI) tissue	44:91	A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures.
27773574	6	54	theme	individual	919:928	arg1	glycosaminoglycans					930:947	the individual glycosaminoglycans	915:947	the individual glycosaminoglycans	915:947	Fluorophore-assisted carbohydrate electrophoresis (FACE) was used to quantitatively assess the individual glycosaminoglycans.
27773574	5	55	theme	METHODS	664:670	arg1	Biopsies					672:679	METHODS Biopsies	664:679	METHODS Biopsies	664:679	METHODS Biopsies were taken from one patient (25years old) at 12months and 20months post-ACI-treatment and from three normal cadavers (21, 22 and 25years old).
27773574	4	56	from	glycosaminoglycans	607:624	arg1	tissue					633:638	ACI tissue	629:638	ACI tissue	629:638	The aim of this study was to assess glycosaminoglycans in ACI tissue over a two year period.
27773574	1	57	used	used	186:189	arg2	implantation					158:169	BACKGROUND Autologous chondrocyte implantation	124:169	BACKGROUND Autologous chondrocyte implantation (ACI)	124:175	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	1	57	used	used	186:189	arg2	ACI					172:174	ACI	172:174	ACI	172:174	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	9	58	theme	chondroitin	1302:1312	arg1	sulphate					1314:1321	chondroitin sulphate	1302:1321	chondroitin sulphate chain length	1302:1334	However, chondroitin sulphate chain length doubled as the ACI repair tissue matured at 12months (3913Da±464) and 20months (6923Da±711) and there was less keratan sulphate as compared to the controls.
27773574	8	59	theme	cadaveric	1273:1281	arg1	biopsies					1283:1290	the age-matched cadaveric biopsies	1257:1290	the age-matched cadaveric biopsies	1257:1290	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	5	60	theme	normal	782:787	arg1	cadavers					789:796	three normal cadavers	776:796	three normal cadavers (21, 22 and 25years old)	776:821	METHODS Biopsies were taken from one patient (25years old) at 12months and 20months post-ACI-treatment and from three normal cadavers (21, 22 and 25years old).
27773574	9	61	theme	repair	1355:1360	arg1	tissue					1362:1367	the ACI repair tissue	1347:1367	the ACI repair tissue	1347:1367	However, chondroitin sulphate chain length doubled as the ACI repair tissue matured at 12months (3913Da±464) and 20months (6923Da±711) and there was less keratan sulphate as compared to the controls.
27773574	7	62	theme	cadaveric	1030:1038	arg1	biopsies					1040:1047	the age-matched cadaveric biopsies	1014:1047	the age-matched cadaveric biopsies	1014:1047	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	8	63	theme	age-matched	1261:1271	arg1	biopsies					1283:1290	the age-matched cadaveric biopsies	1257:1290	the age-matched cadaveric biopsies	1257:1290	Both the 12 and 20month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies.
27773574	10	64	dep	CONCLUSIONS	1493:1503	arg1	identical					1576:1584	identical	1576:1584	identical	1576:1584	CONCLUSIONS Although the glycosaminoglycan composition of the repair tissue is not identical to mature articular cartilage its quality continues to improve with time.
27773574	10	64	dep	CONCLUSIONS	1493:1503	arg1	composition					1536:1546	the glycosaminoglycan composition	1514:1546	the glycosaminoglycan composition of the repair tissue	1514:1567	CONCLUSIONS Although the glycosaminoglycan composition of the repair tissue is not identical to mature articular cartilage its quality continues to improve with time.
27773574	10	65	dep	cartilage	1606:1614	arg1	quality					1620:1626	its quality	1616:1626	mature articular cartilage its quality	1589:1626	CONCLUSIONS Although the glycosaminoglycan composition of the repair tissue is not identical to mature articular cartilage its quality continues to improve with time.
27773574	1	66	theme	cartilage	200:208	arg1	defects					210:216	cartilage defects	200:216	cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation	200:314	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	1	67	from	defects	210:216	arg1	thousands					221:229	thousands	221:229	thousands of patients worldwide with good clinical effectiveness 10-20years after implantation	221:314	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	0	68	dep	study	7:11	arg1	improve					93:99	improve	93:99	improve as the tissue matures	93:121	A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures.
27773574	7	69	contain	had	985:987	arg2	hyaluronan					998:1007	40% less hyaluronan	989:1007	40% less hyaluronan	989:1007	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	7	69	contain	had	985:987	arg1	RESULTS					950:956	RESULTS	950:956	RESULTS At 12months the ACI biopsy	950:983	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	2	70	theme	cartilage	366:374	arg1	quality					344:350	the quality	340:350	the quality of the repair cartilage	340:374	Information concerning the quality of the repair cartilage is still limited because biopsies are small and rare.
27773574	7	71	contain	had	1080:1082	arg1	biopsy					1073:1078	the ACI biopsy	1065:1078	the ACI biopsy	1065:1078	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	7	71	contain	had	1080:1082	arg2	hyaluronan					1103:1112	hyaluronan	1103:1112	hyaluronan	1103:1112	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	7	71	contain	had	1080:1082	arg2	amount					1093:1098	the same amount	1084:1098	the same amount of hyaluronan	1084:1112	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	7	71	contain	had	1080:1082	arg2	controls					1121:1128	the controls	1117:1128	the controls	1117:1128	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	0	72	theme	implantation	67:78	arg1	tissue					86:91	autologous chondrocyte implantation (ACI) tissue	44:91	autologous chondrocyte implantation (ACI) tissue	44:91	A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures.
27773574	9	73	theme	keratan	1447:1453	arg1	sulphate					1455:1462	less keratan sulphate	1442:1462	less keratan sulphate	1442:1462	However, chondroitin sulphate chain length doubled as the ACI repair tissue matured at 12months (3913Da±464) and 20months (6923Da±711) and there was less keratan sulphate as compared to the controls.
27773574	10	74	theme	mature	1589:1594	arg1	cartilage					1606:1614	mature articular cartilage its quality	1589:1626	mature articular cartilage its quality	1589:1626	CONCLUSIONS Although the glycosaminoglycan composition of the repair tissue is not identical to mature articular cartilage its quality continues to improve with time.
27773574	1	75	theme	BACKGROUND	124:133	arg1	ACI					172:174	ACI	172:174	ACI	172:174	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	1	75	theme	BACKGROUND	124:133	arg1	implantation					158:169	BACKGROUND Autologous chondrocyte implantation	124:169	BACKGROUND Autologous chondrocyte implantation (ACI)	124:175	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	7	76	dep	12months	961:968	arg1	biopsy					978:983	the ACI biopsy	970:983	12months the ACI biopsy	961:983	RESULTS At 12months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20months the ACI biopsy had the same amount of hyaluronan as the controls.
27773574	0	77	theme	chondrocyte	55:65	arg1	tissue					86:91	autologous chondrocyte implantation (ACI) tissue	44:91	autologous chondrocyte implantation (ACI) tissue	44:91	A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improve as the tissue matures.
27773574	1	78	theme	Autologous	135:144	arg1	ACI					172:174	ACI	172:174	ACI	172:174	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	1	78	theme	Autologous	135:144	arg1	implantation					158:169	BACKGROUND Autologous chondrocyte implantation	124:169	BACKGROUND Autologous chondrocyte implantation (ACI)	124:175	BACKGROUND Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10-20years after implantation.
27773574	3	79	from	stability	539:547	arg1	important					512:520	important	512:520	important	512:520	Glycosaminoglycan structure influences physiological function and is likely to be important in the long term stability of the repair tissue.
27773574	9	80	theme	ACI	1351:1353	arg1	tissue					1362:1367	the ACI repair tissue	1347:1367	the ACI repair tissue	1347:1367	However, chondroitin sulphate chain length doubled as the ACI repair tissue matured at 12months (3913Da±464) and 20months (6923Da±711) and there was less keratan sulphate as compared to the controls.
26908047	8	0	theme	"	1137:1137	arg1	water					1148:1152	"extra" unfrozen water	1131:1152	"extra" unfrozen water	1131:1152	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	4	1	theme	trehalose	491:499	arg1	ratio					501:505	trehalose ratio	491:505	trehalose ratio (R)	491:509	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	4	1	theme	trehalose	491:499	arg1	R					508:508	R	508:508	R	508:508	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	9	2	theme	freeze-concentrate	1293:1310	arg1	temperature					1274:1284	glass transition temperature	1257:1284	glass transition temperature of the freeze-concentrate	1257:1310	When heated above Tg", crystallization of mannitol and the associated unfrozen water resulted in Tg' (glass transition temperature of the freeze-concentrate).
26908047	4	3	theme	Differential	565:576	arg1	Calorimetry					587:597	Differential Scanning Calorimetry	565:597	Differential Scanning Calorimetry	565:597	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	0	4	theme	Frozen	74:79	arg1	Solutions					81:89	Frozen Solutions	74:89	Frozen Solutions	74:89	Mutual Influence of Mannitol and Trehalose on Crystallization Behavior in Frozen Solutions.
26908047	0	5	from	Influence	7:15	arg1	Behavior					62:69	Crystallization Behavior	46:69	Crystallization Behavior in Frozen Solutions	46:89	Mutual Influence of Mannitol and Trehalose on Crystallization Behavior in Frozen Solutions.
26908047	9	6	theme	unfrozen	1225:1232	arg1	water					1234:1238	the associated unfrozen water	1210:1238	the associated unfrozen water	1210:1238	When heated above Tg", crystallization of mannitol and the associated unfrozen water resulted in Tg' (glass transition temperature of the freeze-concentrate).
26908047	9	7	dep	Tg	1252:1253	arg1	temperature					1274:1284	glass transition temperature	1257:1284	glass transition temperature of the freeze-concentrate	1257:1310	When heated above Tg", crystallization of mannitol and the associated unfrozen water resulted in Tg' (glass transition temperature of the freeze-concentrate).
26908047	9	8	theme	transition	1263:1272	arg1	temperature					1274:1284	glass transition temperature	1257:1284	glass transition temperature of the freeze-concentrate	1257:1310	When heated above Tg", crystallization of mannitol and the associated unfrozen water resulted in Tg' (glass transition temperature of the freeze-concentrate).
26908047	10	9	theme	solute	1346:1351	arg1	concentration					1353:1365	the total solute concentration	1336:1365	the total solute concentration	1336:1365	CONCLUSIONS R and not the total solute concentration, dictates the composition of the freeze concentrate as well as the physical stability of the excipients.
26908047	8	10	theme	unfrozen	1139:1146	arg1	water					1148:1152	"extra" unfrozen water	1131:1152	"extra" unfrozen water	1131:1152	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	6	11	theme	R	890:890	arg1	value					881:885	the value	877:885	the value of R	877:890	When R ≥ 1, extent of mannitol crystallization was directly proportional to the value of R.
26908047	8	12	theme	glass	1039:1043	arg1	transitions					1045:1055	two glass transitions	1035:1055	two glass transitions	1035:1055	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	8	12	theme	glass	1039:1043	arg1	"					1095:1095	Tg"	1093:1095	Tg"	1093:1095	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	9	13	theme	Tg	1173:1174	arg1	"					1175:1175	Tg"	1173:1175	Tg"	1173:1175	When heated above Tg", crystallization of mannitol and the associated unfrozen water resulted in Tg' (glass transition temperature of the freeze-concentrate).
26908047	4	14	theme	Scanning	578:585	arg1	Calorimetry					587:597	Differential Scanning Calorimetry	565:597	Differential Scanning Calorimetry	565:597	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	3	15	theme	mutual	334:339	arg1	interaction					341:351	the mutual interaction	330:351	the mutual interaction of these sugars	330:367	It is therefore imperative to understand the mutual interaction of these sugars during freezing to ensure preservation of the API during freeze-drying.
26908047	8	16	theme	freeze	977:982	arg1	concentrate					984:994	the freeze concentrate	973:994	the freeze concentrate	973:994	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	4	17	dep	METHODS	441:447	arg1	investigated					452:463	investigated	452:463	investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing	452:682	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	10	18	theme	physical	1434:1441	arg1	stability					1443:1451	the physical stability	1430:1451	the composition of the freeze concentrate as well as the physical stability of the excipients	1377:1469	CONCLUSIONS R and not the total solute concentration, dictates the composition of the freeze concentrate as well as the physical stability of the excipients.
26908047	9	19	theme	associated	1214:1223	arg1	water					1234:1238	the associated unfrozen water	1210:1238	the associated unfrozen water	1210:1238	When heated above Tg", crystallization of mannitol and the associated unfrozen water resulted in Tg' (glass transition temperature of the freeze-concentrate).
26908047	4	20	theme	mannitol	479:486	arg1	effect					469:474	the effect	465:474	the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes	465:557	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	8	21	theme	temperature	1069:1079	arg1	transition					1081:1090	the lower temperature transition	1059:1090	the lower temperature transition	1059:1090	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	7	22	theme	mannitol	937:944	arg1	crystallization					946:960	mannitol crystallization	937:960	mannitol crystallization	937:960	When R < 1, trehalose completely suppressed mannitol crystallization.
26908047	3	23	theme	API	415:417	arg1	preservation					395:406	preservation	395:406	preservation of the API	395:417	It is therefore imperative to understand the mutual interaction of these sugars during freezing to ensure preservation of the API during freeze-drying.
26908047	0	24	theme	Mutual	0:5	arg1	Influence					7:15	Mutual Influence	0:15	Mutual Influence of Mannitol and Trehalose on Crystallization Behavior in Frozen Solutions	0:89	Mutual Influence of Mannitol and Trehalose on Crystallization Behavior in Frozen Solutions.
26908047	10	25	theme	freeze	1400:1405	arg1	concentrate					1407:1417	the freeze concentrate	1396:1417	the freeze concentrate	1396:1417	CONCLUSIONS R and not the total solute concentration, dictates the composition of the freeze concentrate as well as the physical stability of the excipients.
26908047	4	26	from	effect	469:474	arg1	behavior					534:541	the crystallization behavior	514:541	the crystallization behavior of both solutes	514:557	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	1	27	theme	PURPOSE	92:98	arg1	separation					106:115	PURPOSE Phase separation	92:115	PURPOSE Phase separation of trehalose during freeze-drying	92:149	PURPOSE Phase separation of trehalose during freeze-drying could render it ineffective as a lyoprotectant.
26908047	3	28	theme	sugars	362:367	arg1	interaction					341:351	the mutual interaction	330:351	the mutual interaction of these sugars	330:367	It is therefore imperative to understand the mutual interaction of these sugars during freezing to ensure preservation of the API during freeze-drying.
26908047	10	29	theme	concentrate	1407:1417	arg1	composition					1381:1391	the composition	1377:1391	the composition of the freeze concentrate as well as the physical stability of the excipients	1377:1469	CONCLUSIONS R and not the total solute concentration, dictates the composition of the freeze concentrate as well as the physical stability of the excipients.
26908047	10	29	theme	concentrate	1407:1417	arg1	stability					1443:1451	the physical stability	1430:1451	the composition of the freeze concentrate as well as the physical stability of the excipients	1377:1469	CONCLUSIONS R and not the total solute concentration, dictates the composition of the freeze concentrate as well as the physical stability of the excipients.
26908047	9	30	theme	mannitol	1197:1204	arg1	crystallization					1178:1192	crystallization	1178:1192	crystallization of mannitol and the associated unfrozen water	1178:1238	When heated above Tg", crystallization of mannitol and the associated unfrozen water resulted in Tg' (glass transition temperature of the freeze-concentrate).
26908047	1	31	theme	Phase	100:104	arg1	separation					106:115	PURPOSE Phase separation	92:115	PURPOSE Phase separation of trehalose during freeze-drying	92:149	PURPOSE Phase separation of trehalose during freeze-drying could render it ineffective as a lyoprotectant.
26908047	8	32	from	R > 1	966:970	arg1	heterogeneous					1000:1012	heterogeneous	1000:1012	heterogeneous	1000:1012	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	4	33	theme	solutes	551:557	arg1	behavior					534:541	the crystallization behavior	514:541	the crystallization behavior of both solutes	514:557	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	10	34	theme	excipients	1460:1469	arg1	composition					1381:1391	the composition	1377:1391	the composition of the freeze concentrate as well as the physical stability of the excipients	1377:1469	CONCLUSIONS R and not the total solute concentration, dictates the composition of the freeze concentrate as well as the physical stability of the excipients.
26908047	10	34	theme	excipients	1460:1469	arg1	stability					1443:1451	the physical stability	1430:1451	the composition of the freeze concentrate as well as the physical stability of the excipients	1377:1469	CONCLUSIONS R and not the total solute concentration, dictates the composition of the freeze concentrate as well as the physical stability of the excipients.
26908047	4	35	theme	FTIR	626:629	arg1	Spectrosopy					631:641	FTIR Spectrosopy	626:641	FTIR Spectrosopy	626:641	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	0	36	theme	Trehalose	33:41	arg1	Influence					7:15	Mutual Influence	0:15	Mutual Influence of Mannitol and Trehalose on Crystallization Behavior in Frozen Solutions	0:89	Mutual Influence of Mannitol and Trehalose on Crystallization Behavior in Frozen Solutions.
26908047	4	37	theme	controlled	650:659	arg1	freezing					661:668	controlled freezing	650:668	controlled freezing	650:668	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	5	38	theme	mannitol	729:736	arg1	crystallization					705:719	R = 1, crystallization	698:719	crystallization	705:719	RESULTS When R = 1, crystallization of both mannitol (as hemihydrate) and trehalose (as α-anhydrate) were observed.
26908047	8	39	theme	extra	1132:1136	arg1	water					1148:1152	"extra" unfrozen water	1131:1152	"extra" unfrozen water	1131:1152	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	0	40	theme	Mannitol	20:27	arg1	Influence					7:15	Mutual Influence	0:15	Mutual Influence of Mannitol and Trehalose on Crystallization Behavior in Frozen Solutions	0:89	Mutual Influence of Mannitol and Trehalose on Crystallization Behavior in Frozen Solutions.
26908047	0	41	theme	Crystallization	46:60	arg1	Behavior					62:69	Crystallization Behavior	46:69	Crystallization Behavior in Frozen Solutions	46:89	Mutual Influence of Mannitol and Trehalose on Crystallization Behavior in Frozen Solutions.
26908047	0	42	from	Behavior	62:69	arg1	Solutions					81:89	Frozen Solutions	74:89	Frozen Solutions	74:89	Mutual Influence of Mannitol and Trehalose on Crystallization Behavior in Frozen Solutions.
26908047	10	43	theme	CONCLUSIONS	1314:1324	arg1	R					1326:1326	CONCLUSIONS R	1314:1326	CONCLUSIONS R	1314:1326	CONCLUSIONS R and not the total solute concentration, dictates the composition of the freeze concentrate as well as the physical stability of the excipients.
26908047	8	44	theme	Tg	1093:1094	arg1	"					1095:1095	Tg"	1093:1095	Tg"	1093:1095	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	8	44	theme	Tg	1093:1094	arg1	transitions					1045:1055	two glass transitions	1035:1055	two glass transitions	1035:1055	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	4	45	theme	crystallization	518:532	arg1	behavior					534:541	the crystallization behavior	514:541	the crystallization behavior of both solutes	514:557	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	6	46	theme	crystallization	832:846	arg1	extent					813:818	extent	813:818	extent of mannitol crystallization	813:846	When R ≥ 1, extent of mannitol crystallization was directly proportional to the value of R.
26908047	1	47	theme	trehalose	120:128	arg1	separation					106:115	PURPOSE Phase separation	92:115	PURPOSE Phase separation of trehalose during freeze-drying	92:149	PURPOSE Phase separation of trehalose during freeze-drying could render it ineffective as a lyoprotectant.
26908047	5	48	theme	R = 1	698:702	arg1	crystallization					705:719	R = 1, crystallization	698:719	crystallization	705:719	RESULTS When R = 1, crystallization of both mannitol (as hemihydrate) and trehalose (as α-anhydrate) were observed.
26908047	6	49	theme	mannitol	823:830	arg1	crystallization					832:846	mannitol crystallization	823:846	mannitol crystallization	823:846	When R ≥ 1, extent of mannitol crystallization was directly proportional to the value of R.
26908047	8	50	contain	containing	1120:1129	arg2	water					1148:1152	"extra" unfrozen water	1131:1152	"extra" unfrozen water	1131:1152	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	8	50	contain	containing	1120:1129	arg1	systems					1112:1118	systems	1112:1118	systems containing "extra" unfrozen water	1112:1152	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	4	51	theme	X-Ray	600:604	arg1	Crystallography					606:620	X-Ray Crystallography	600:620	X-Ray Crystallography	600:620	METHODS We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing.
26908047	2	52	theme	bulking	203:209	arg1	mannitol					218:225	mannitol	218:225	mannitol	218:225	The bulking agent, mannitol, on the other hand, should crystallize readily upon freezing.
26908047	2	52	theme	bulking	203:209	arg1	agent					211:215	The bulking agent	199:215	The bulking agent	199:215	The bulking agent, mannitol, on the other hand, should crystallize readily upon freezing.
26908047	10	53	theme	total	1340:1344	arg1	concentration					1353:1365	the total solute concentration	1336:1365	the total solute concentration	1336:1365	CONCLUSIONS R and not the total solute concentration, dictates the composition of the freeze concentrate as well as the physical stability of the excipients.
26908047	9	54	theme	water	1234:1238	arg1	crystallization					1178:1192	crystallization	1178:1192	crystallization of mannitol and the associated unfrozen water	1178:1238	When heated above Tg", crystallization of mannitol and the associated unfrozen water resulted in Tg' (glass transition temperature of the freeze-concentrate).
26908047	8	55	theme	lower	1063:1067	arg1	transition					1081:1090	the lower temperature transition	1059:1090	the lower temperature transition	1059:1090	At R > 1, the freeze concentrate was heterogeneous and characterized by two glass transitions - the lower temperature transition (Tg") attributed to systems containing "extra" unfrozen water.
26908047	5	56	theme	trehalose	759:767	arg1	crystallization					705:719	R = 1, crystallization	698:719	crystallization	705:719	RESULTS When R = 1, crystallization of both mannitol (as hemihydrate) and trehalose (as α-anhydrate) were observed.
26908047	9	57	theme	glass	1257:1261	arg1	transition					1263:1272	glass transition	1257:1272	glass transition temperature of the freeze-concentrate	1257:1310	When heated above Tg", crystallization of mannitol and the associated unfrozen water resulted in Tg' (glass transition temperature of the freeze-concentrate).
26908047	2	58	theme	other	235:239	arg1	hand					241:244	the other hand	231:244	the other hand	231:244	The bulking agent, mannitol, on the other hand, should crystallize readily upon freezing.
27481343	0	0	theme	alginate	48:55	arg1	material					67:74	alginate composite material	48:74	alginate composite material	48:74	Pb(II) adsorption by a novel activated carbon - alginate composite material.
27481343	6	1	theme	adsorption	826:835	arg1	qm					847:848	qm	847:848	qm	847:848	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	6	1	theme	adsorption	826:835	arg1	capacity					837:844	the adsorption capacity	822:844	the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material	822:905	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	2	2	theme	Pb	302:303	arg1	ion					309:311	Pb(II) ion	302:311	Pb(II) ion from aqueous solution	302:333	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	6	3	theme	active	854:859	arg1	AC					869:870	AC	869:870	AC	869:870	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	6	3	theme	active	854:859	arg1	carbon					861:866	active carbon	854:866	active carbon (AC)	854:871	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	6	4	theme	Calcium	788:794	arg1	AA-Ca					806:810	AA-Ca	806:810	AA-Ca	806:810	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	6	4	theme	Calcium	788:794	arg1	alginate					796:803	Calcium alginate	788:803	Calcium alginate (AA-Ca)	788:811	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	3	5	theme	strength	403:410	arg1	0.1-0.75molL-1					418:431	the ionic strength range 0.1-0.75molL-1	393:431	the ionic strength range 0.1-0.75molL-1	393:431	The study was carried out at pH=5, in NaCl medium and in the ionic strength range 0.1-0.75molL-1.
27481343	5	6	theme	pseudo	615:620	arg1	order					628:632	pseudo first order	615:632	pseudo first order	615:632	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	8	7	theme	specific	1277:1284	arg1	contribution					1286:1297	the specific contribution	1273:1297	the specific contribution of AC and AA-Ca to the adsorption of the metal ion	1273:1348	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	5	8	used	used	724:727	arg2	Freundlich					700:709	Freundlich	700:709	Freundlich	700:709	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	5	8	used	used	724:727	arg2	Langmuir					687:694	Langmuir	687:694	Langmuir	687:694	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	5	8	used	used	724:727	arg2	kinetic					606:612	kinetic	606:612	kinetic	606:612	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	5	8	used	used	724:727	arg2	models					712:717	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models	596:717	models	712:717	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	8	9	from	solution	1153:1160	arg1	results					1107:1113	The results	1103:1113	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC	1103:1236	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	0	10	theme	composite	57:65	arg1	material					67:74	alginate composite material	48:74	alginate composite material	48:74	Pb(II) adsorption by a novel activated carbon - alginate composite material.
27481343	8	11	theme	AC	1302:1303	arg1	contribution					1286:1297	the specific contribution	1273:1297	the specific contribution of AC and AA-Ca to the adsorption of the metal ion	1273:1348	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	8	12	theme	Pb	1146:1147	arg1	solution					1153:1160	the Pb(II) solution	1142:1160	the Pb(II) solution	1142:1160	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	8	13	from	results	1107:1113	arg1	solution					1153:1160	the Pb(II) solution	1142:1160	the Pb(II) solution	1142:1160	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	3	14	theme	ionic	397:401	arg1	0.1-0.75molL-1					418:431	the ionic strength range 0.1-0.75molL-1	393:431	the ionic strength range 0.1-0.75molL-1	393:431	The study was carried out at pH=5, in NaCl medium and in the ionic strength range 0.1-0.75molL-1.
27481343	6	15	theme	carbon	861:866	arg1	qm					847:848	qm	847:848	qm	847:848	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	6	15	theme	carbon	861:866	arg1	capacity					837:844	the adsorption capacity	822:844	the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material	822:905	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	7	16	theme	thermogravimetric	1000:1016	arg1	measurements					1024:1035	SEM-EDX and thermogravimetric (TGA) measurements	988:1035	SEM-EDX and thermogravimetric (TGA) measurements	988:1035	SEM-EDX and thermogravimetric (TGA) measurements were carried out in order to characterize the composite material.
27481343	2	17	theme	adsorption	114:123	arg1	capacity					125:132	The adsorption capacity	110:132	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca)	110:203	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	6	18	theme	ACAA-Ca	880:886	arg1	material					898:905	the ACAA-Ca adsorbent material	876:905	the ACAA-Ca adsorbent material	876:905	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	2	19	theme	aqueous	318:324	arg1	solution					326:333	aqueous solution	318:333	aqueous solution	318:333	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	8	20	theme	AA-Ca	1309:1313	arg1	contribution					1286:1297	the specific contribution	1273:1297	the specific contribution of AC and AA-Ca to the adsorption of the metal ion	1273:1348	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	4	21	dep	kinetic	559:565	arg1	experiments					583:593	experiments	583:593	experiments	583:593	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique was used to check the amount of Pb(II) ion removed during kinetic and equilibrium experiments.
27481343	7	22	theme	SEM-EDX	988:994	arg1	measurements					1024:1035	SEM-EDX and thermogravimetric (TGA) measurements	988:1035	SEM-EDX and thermogravimetric (TGA) measurements	988:1035	SEM-EDX and thermogravimetric (TGA) measurements were carried out in order to characterize the composite material.
27481343	5	23	dep	kinetic	606:612	arg1	order					628:632	pseudo first order	615:632	pseudo first order	615:632	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	5	23	dep	kinetic	606:612	arg1	Vermuelen					659:667	Vermuelen	659:667	Vermuelen	659:667	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	5	23	dep	kinetic	606:612	arg1	order					649:653	pseudo second order	635:653	pseudo second order	635:653	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	7	24	theme	composite	1083:1091	arg1	material					1093:1100	the composite material	1079:1100	the composite material	1079:1100	SEM-EDX and thermogravimetric (TGA) measurements were carried out in order to characterize the composite material.
27481343	5	25	theme	experimental	736:747	arg1	data					749:752	experimental data	736:752	experimental data	736:752	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	0	26	theme	Pb	0:1	arg1	adsorption					7:16	Pb(II) adsorption	0:16	Pb(II) adsorption by a novel	0:27	Pb(II) adsorption by a novel activated carbon - alginate composite material.
27481343	4	27	theme	Voltammetry	470:480	arg1	technique					491:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique was used to check the amount of Pb(II) ion removed during kinetic and equilibrium experiments.
27481343	8	28	from	study	1133:1137	arg1	solution					1153:1160	the Pb(II) solution	1142:1160	the Pb(II) solution	1142:1160	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	8	29	theme	AA-Ca	1225:1229	arg1	results					1107:1113	The results	1103:1113	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC	1103:1236	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	4	30	theme	Stripping	460:468	arg1	technique					491:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique was used to check the amount of Pb(II) ion removed during kinetic and equilibrium experiments.
27481343	2	31	theme	new	250:252	arg1	material					283:290	a new and more efficient adsorbent material	248:290	a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution	248:333	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	4	32	theme	Pb	533:534	arg1	ion					540:542	Pb(II) ion	533:542	Pb(II) ion	533:542	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique was used to check the amount of Pb(II) ion removed during kinetic and equilibrium experiments.
27481343	4	33	theme	DP-ASV	483:488	arg1	technique					491:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique was used to check the amount of Pb(II) ion removed during kinetic and equilibrium experiments.
27481343	2	34	theme	carbon	150:155	arg1	capacity					125:132	The adsorption capacity	110:132	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca)	110:203	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	3	35	theme	NaCl	374:377	arg1	medium					379:384	NaCl medium	374:384	NaCl medium	374:384	The study was carried out at pH=5, in NaCl medium and in the ionic strength range 0.1-0.75molL-1.
27481343	7	36	dep	carried	1042:1048	arg1	out					1050:1052	out	1050:1052	out	1050:1052	SEM-EDX and thermogravimetric (TGA) measurements were carried out in order to characterize the composite material.
27481343	2	37	theme	activated	140:148	arg1	carbon					150:155	an activated carbon	137:155	an activated carbon	137:155	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	8	38	theme	study	1133:1137	arg1	results					1107:1113	The results	1103:1113	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC	1103:1236	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	4	39	theme	ion	540:542	arg1	ion					540:542	Pb(II) ion	533:542	Pb(II) ion	533:542	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique was used to check the amount of Pb(II) ion removed during kinetic and equilibrium experiments.
27481343	4	39	theme	ion	540:542	arg1	amount					523:528	the amount	519:528	the amount of Pb(II) ion removed during kinetic and equilibrium experiments	519:593	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique was used to check the amount of Pb(II) ion removed during kinetic and equilibrium experiments.
27481343	4	40	theme	Differential	434:445	arg1	technique					491:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique was used to check the amount of Pb(II) ion removed during kinetic and equilibrium experiments.
27481343	6	41	from	capacity	837:844	arg1	material					898:905	the ACAA-Ca adsorbent material	876:905	the ACAA-Ca adsorbent material	876:905	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	6	42	dep	improves	813:820	arg1	10.5mgg-1					926:934	10.5mgg-1	926:934	10.5mgg-1	926:934	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	6	42	dep	improves	813:820	arg1	qm=15.7					914:920	qm=15.7	914:920	qm=15.7	914:920	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	6	43	dep	qm=15.7	914:920	arg1	e.g.					908:911	e.g.	908:911	e.g.	908:911	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	8	44	theme	ion	1346:1348	arg1	adsorption					1322:1331	the adsorption	1318:1331	the adsorption of the metal ion	1318:1348	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	8	45	theme	metal	1340:1344	arg1	ion					1346:1348	the metal ion	1336:1348	the metal ion	1336:1348	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	8	46	theme	AC	1235:1236	arg1	results					1107:1113	The results	1103:1113	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC	1103:1236	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	8	47	theme	ACAA-Ca	1197:1203	arg1	characterization					1173:1188	the characterization	1169:1188	the characterization of the ACAA-Ca	1169:1203	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	6	48	from	I=0.25molL-1	939:950	arg1	10.5mgg-1					926:934	10.5mgg-1	926:934	10.5mgg-1	926:934	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	6	48	from	I=0.25molL-1	939:950	arg1	qm=15.7					914:920	qm=15.7	914:920	qm=15.7	914:920	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	5	49	theme	equilibrium	674:684	arg1	Langmuir					687:694	Langmuir	687:694	Langmuir	687:694	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	5	49	theme	equilibrium	674:684	arg1	models					712:717	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models	596:717	models	712:717	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	5	49	theme	equilibrium	674:684	arg1	Freundlich					700:709	Freundlich	700:709	Freundlich	700:709	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	4	50	theme	Anodic	453:458	arg1	technique					491:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique was used to check the amount of Pb(II) ion removed during kinetic and equilibrium experiments.
27481343	4	51	used	used	505:508	arg2	technique					491:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique was used to check the amount of Pb(II) ion removed during kinetic and equilibrium experiments.
27481343	2	52	theme	composite	176:184	arg1	ACAA-Ca					196:202	ACAA-Ca	196:202	ACAA-Ca	196:202	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	2	52	theme	composite	176:184	arg1	material					186:193	calcium alginate composite material	159:193	calcium alginate composite material (ACAA-Ca)	159:203	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	2	53	theme	material	186:193	arg1	capacity					125:132	The adsorption capacity	110:132	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca)	110:203	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	4	54	theme	Pulse	447:451	arg1	technique					491:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique	434:499	Differential Pulse Anodic Stripping Voltammetry (DP-ASV) technique was used to check the amount of Pb(II) ion removed during kinetic and equilibrium experiments.
27481343	2	55	theme	alginate	167:174	arg1	ACAA-Ca					196:202	ACAA-Ca	196:202	ACAA-Ca	196:202	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	2	55	theme	alginate	167:174	arg1	material					186:193	calcium alginate composite material	159:193	calcium alginate composite material (ACAA-Ca)	159:203	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	8	56	theme	characterization	1173:1188	arg1	results					1107:1113	The results	1103:1113	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC	1103:1236	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	2	57	theme	adsorbent	273:281	arg1	material					283:290	a new and more efficient adsorbent material	248:290	a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution	248:333	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	2	58	theme	calcium	159:165	arg1	ACAA-Ca					196:202	ACAA-Ca	196:202	ACAA-Ca	196:202	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	2	58	theme	calcium	159:165	arg1	material					186:193	calcium alginate composite material	159:193	calcium alginate composite material (ACAA-Ca)	159:203	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	6	59	theme	adsorbent	888:896	arg1	material					898:905	the ACAA-Ca adsorbent material	876:905	the ACAA-Ca adsorbent material	876:905	Calcium alginate (AA-Ca) improves the adsorption capacity (qm) of active carbon (AC) in the ACAA-Ca adsorbent material (e.g., qm=15.7 and 10.5mgg-1 at I=0.25molL-1, for ACAA-Ca and AC, respectively).
27481343	2	60	theme	efficient	263:271	arg1	material					283:290	a new and more efficient adsorbent material	248:290	a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution	248:333	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	5	61	theme	pseudo	635:640	arg1	order					649:653	pseudo second order	635:653	pseudo second order	635:653	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	8	62	theme	pristine	1216:1223	arg1	AA-Ca					1225:1229	the pristine AA-Ca	1212:1229	the pristine AA-Ca	1212:1229	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	2	63	from	solution	326:333	arg1	ion					309:311	Pb(II) ion	302:311	Pb(II) ion from aqueous solution	302:333	The adsorption capacity of an activated carbon - calcium alginate composite material (ACAA-Ca) has been tested with the aim of developing a new and more efficient adsorbent material to remove Pb(II) ion from aqueous solution.
27481343	3	64	theme	range	412:416	arg1	0.1-0.75molL-1					418:431	the ionic strength range 0.1-0.75molL-1	393:431	the ionic strength range 0.1-0.75molL-1	393:431	The study was carried out at pH=5, in NaCl medium and in the ionic strength range 0.1-0.75molL-1.
27481343	5	65	theme	second	642:647	arg1	order					649:653	pseudo second order	635:653	pseudo second order	635:653	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	5	66	dep	models	712:717	arg1	Langmuir					687:694	Langmuir	687:694	Langmuir	687:694	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	5	66	dep	models	712:717	arg1	models					712:717	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models	596:717	models	712:717	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	5	66	dep	models	712:717	arg1	Freundlich					700:709	Freundlich	700:709	Freundlich	700:709	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
27481343	8	67	theme	speciation	1122:1131	arg1	study					1133:1137	the speciation study	1118:1137	the speciation study on the Pb(II) solution	1118:1160	The results of the speciation study on the Pb(II) solution and of the characterization of the ACAA-Ca and of the pristine AA-Ca and AC were evaluated in order to explain the specific contribution of AC and AA-Ca to the adsorption of the metal ion.
27481343	5	68	theme	first	622:626	arg1	order					628:632	pseudo first order	615:632	pseudo first order	615:632	Different kinetic (pseudo first order, pseudo second order and Vermuelen) and equilibrium (Langmuir and Freundlich) models were used to fit experimental data, and were statistically compared.
25666412	0	0	theme	durum	73:77	arg1	spaghetti					85:93	durum wheat spaghetti	73:93	durum wheat spaghetti	73:93	Effect of raw material on cooking quality and nutritional composition of durum wheat spaghetti.
25666412	2	1	from	decline	393:399	arg1	loss					438:441	high cooking loss	425:441	high cooking loss	425:441	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	2	1	from	decline	393:399	arg1	quality					416:422	the cooking quality	404:422	the cooking quality (high cooking loss)	404:442	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	4	2	theme	other	648:652	arg1	samples					654:660	the other samples	644:660	the other samples	644:660	As compared to the other samples, the wholemeal spaghetti Iride recorded a higher cooking loss.
25666412	5	3	theme	firmness	829:836	arg1	score					808:812	the low score	800:812	the low score of elasticity, firmness and colour	800:847	Moreover, the wholemeal spaghetti showed the lowest overall quality due to the low score of elasticity, firmness and colour.
25666412	1	4	theme	wholemeal	137:145	arg1	flour					147:151	wholemeal flour	137:151	wholemeal flour	137:151	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	6	5	theme	quality	925:931	arg1	rise					905:908	a slight rise	896:908	a slight rise of the overall quality	896:931	Specifically, the wholemeal Cappelli recorded a slight rise of the overall quality with respect to other wholemeal samples.
25666412	0	6	theme	spaghetti	85:93	arg1	quality					34:40	cooking quality	26:40	cooking quality	26:40	Effect of raw material on cooking quality and nutritional composition of durum wheat spaghetti.
25666412	0	6	theme	spaghetti	85:93	arg1	composition					58:68	nutritional composition	46:68	nutritional composition	46:68	Effect of raw material on cooking quality and nutritional composition of durum wheat spaghetti.
25666412	0	7	from	Effect	0:5	arg1	quality					34:40	cooking quality	26:40	cooking quality	26:40	Effect of raw material on cooking quality and nutritional composition of durum wheat spaghetti.
25666412	0	7	from	Effect	0:5	arg1	composition					58:68	nutritional composition	46:68	nutritional composition	46:68	Effect of raw material on cooking quality and nutritional composition of durum wheat spaghetti.
25666412	4	8	theme	cooking	711:717	arg1	loss					719:722	a higher cooking loss	702:722	a higher cooking loss	702:722	As compared to the other samples, the wholemeal spaghetti Iride recorded a higher cooking loss.
25666412	2	9	from	improvement	286:296	arg1	composition					314:324	the chemical composition	301:324	the chemical composition	301:324	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	6	10	theme	overall	917:923	arg1	quality					925:931	the overall quality	913:931	the overall quality	913:931	Specifically, the wholemeal Cappelli recorded a slight rise of the overall quality with respect to other wholemeal samples.
25666412	2	11	theme	semolina	464:471	arg1	spaghetti					473:481	the semolina spaghetti	460:481	the semolina spaghetti	460:481	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	4	12	theme	higher	704:709	arg1	loss					719:722	a higher cooking loss	702:722	a higher cooking loss	702:722	As compared to the other samples, the wholemeal spaghetti Iride recorded a higher cooking loss.
25666412	7	13	theme	sensory	1072:1078	arg1	quality					1096:1102	the sensory and nutritional quality	1068:1102	the sensory and nutritional quality	1068:1102	In conclusion, the wholemeal spaghetti Cappelli was found to be an optimum compromise between the sensory and nutritional quality.
25666412	2	14	theme	spaghetti	258:266	arg1	samples					268:274	The wholemeal spaghetti samples	244:274	The wholemeal spaghetti samples	244:274	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	2	15	theme	wholemeal	248:256	arg1	samples					268:274	The wholemeal spaghetti samples	244:274	The wholemeal spaghetti samples	244:274	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	7	16	dep	optimum	1041:1047	arg1	compromise					1049:1058	compromise	1049:1058	compromise	1049:1058	In conclusion, the wholemeal spaghetti Cappelli was found to be an optimum compromise between the sensory and nutritional quality.
25666412	1	17	theme	flour	147:151	arg1	effect					114:119	the effect	110:119	the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality	110:227	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	2	18	theme	cooking	430:436	arg1	loss					438:441	high cooking loss	425:441	high cooking loss	425:441	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	2	18	theme	cooking	430:436	arg1	quality					416:422	the cooking quality	404:422	the cooking quality (high cooking loss)	404:442	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	6	19	theme	other	949:953	arg1	samples					965:971	other wholemeal samples	949:971	other wholemeal samples	949:971	Specifically, the wholemeal Cappelli recorded a slight rise of the overall quality with respect to other wholemeal samples.
25666412	5	20	theme	low	804:806	arg1	score					808:812	the low score	800:812	the low score of elasticity, firmness and colour	800:847	Moreover, the wholemeal spaghetti showed the lowest overall quality due to the low score of elasticity, firmness and colour.
25666412	2	21	theme	cooking	408:414	arg1	loss					438:441	high cooking loss	425:441	high cooking loss	425:441	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	2	21	theme	cooking	408:414	arg1	quality					416:422	the cooking quality	404:422	the cooking quality (high cooking loss)	404:442	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	1	22	from	effect	114:119	arg1	cultivars					174:182	six durum wheat cultivars	158:182	six durum wheat cultivars	158:182	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	1	22	from	effect	114:119	arg1	cooking					197:203	the pasta cooking	187:203	the pasta cooking	187:203	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	1	22	from	effect	114:119	arg1	quality					221:227	nutritional quality	209:227	nutritional quality	209:227	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	3	23	theme	spaghetti	513:521	arg1	Cappelli					523:530	the wholemeal spaghetti Cappelli	499:530	the wholemeal spaghetti Cappelli	499:530	In particular, the wholemeal spaghetti Cappelli and Core samples recorded the highest protein and insoluble dietary fibre content, respectively.
25666412	2	24	theme	fibre	362:366	arg1	content					368:374	high protein and insoluble dietary fibre content	327:374	content	368:374	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	6	25	theme	wholemeal	955:963	arg1	samples					965:971	other wholemeal samples	949:971	other wholemeal samples	949:971	Specifically, the wholemeal Cappelli recorded a slight rise of the overall quality with respect to other wholemeal samples.
25666412	3	26	theme	wholemeal	503:511	arg1	Cappelli					523:530	the wholemeal spaghetti Cappelli	499:530	the wholemeal spaghetti Cappelli	499:530	In particular, the wholemeal spaghetti Cappelli and Core samples recorded the highest protein and insoluble dietary fibre content, respectively.
25666412	4	27	theme	spaghetti	677:685	arg1	Iride					687:691	the wholemeal spaghetti Iride	663:691	the wholemeal spaghetti Iride	663:691	As compared to the other samples, the wholemeal spaghetti Iride recorded a higher cooking loss.
25666412	1	28	theme	durum	162:166	arg1	cultivars					174:182	six durum wheat cultivars	158:182	six durum wheat cultivars	158:182	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	0	29	theme	material	14:21	arg1	Effect					0:5	Effect	0:5	Effect of raw material on cooking quality and nutritional composition of durum wheat spaghetti.	0:94	Effect of raw material on cooking quality and nutritional composition of durum wheat spaghetti.
25666412	7	30	theme	wholemeal	993:1001	arg1	Cappelli					1013:1020	the wholemeal spaghetti Cappelli	989:1020	the wholemeal spaghetti Cappelli	989:1020	In conclusion, the wholemeal spaghetti Cappelli was found to be an optimum compromise between the sensory and nutritional quality.
25666412	7	30	theme	wholemeal	993:1001	arg1	optimum					1041:1047	an optimum compromise	1038:1058	an optimum compromise between the sensory and nutritional quality	1038:1102	In conclusion, the wholemeal spaghetti Cappelli was found to be an optimum compromise between the sensory and nutritional quality.
25666412	1	31	theme	wheat	168:172	arg1	cultivars					174:182	six durum wheat cultivars	158:182	six durum wheat cultivars	158:182	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	0	32	theme	raw	10:12	arg1	material					14:21	raw material	10:21	raw material	10:21	Effect of raw material on cooking quality and nutritional composition of durum wheat spaghetti.
25666412	6	33	theme	slight	898:903	arg1	rise					905:908	a slight rise	896:908	a slight rise of the overall quality	896:931	Specifically, the wholemeal Cappelli recorded a slight rise of the overall quality with respect to other wholemeal samples.
25666412	7	34	theme	spaghetti	1003:1011	arg1	Cappelli					1013:1020	the wholemeal spaghetti Cappelli	989:1020	the wholemeal spaghetti Cappelli	989:1020	In conclusion, the wholemeal spaghetti Cappelli was found to be an optimum compromise between the sensory and nutritional quality.
25666412	7	34	theme	spaghetti	1003:1011	arg1	optimum					1041:1047	an optimum compromise	1038:1058	an optimum compromise between the sensory and nutritional quality	1038:1102	In conclusion, the wholemeal spaghetti Cappelli was found to be an optimum compromise between the sensory and nutritional quality.
25666412	3	35	theme	fibre	600:604	arg1	content					606:612	insoluble dietary fibre content	582:612	insoluble dietary fibre content	582:612	In particular, the wholemeal spaghetti Cappelli and Core samples recorded the highest protein and insoluble dietary fibre content, respectively.
25666412	2	36	theme	high	327:330	arg1	protein					332:338	high protein and insoluble dietary fibre content	327:374	protein	332:338	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	5	37	theme	elasticity	817:826	arg1	score					808:812	the low score	800:812	the low score of elasticity, firmness and colour	800:847	Moreover, the wholemeal spaghetti showed the lowest overall quality due to the low score of elasticity, firmness and colour.
25666412	0	38	theme	cooking	26:32	arg1	quality					34:40	cooking quality	26:40	cooking quality	26:40	Effect of raw material on cooking quality and nutritional composition of durum wheat spaghetti.
25666412	2	39	theme	dietary	354:360	arg1	content					368:374	high protein and insoluble dietary fibre content	327:374	content	368:374	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	5	40	theme	due	793:795	arg1	quality					785:791	the lowest overall quality	766:791	the lowest overall quality due to the low score of elasticity, firmness and colour	766:847	Moreover, the wholemeal spaghetti showed the lowest overall quality due to the low score of elasticity, firmness and colour.
25666412	2	41	theme	high	425:428	arg1	loss					438:441	high cooking loss	425:441	high cooking loss	425:441	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	2	41	theme	high	425:428	arg1	quality					416:422	the cooking quality	404:422	the cooking quality (high cooking loss)	404:442	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	3	42	theme	insoluble	582:590	arg1	content					606:612	insoluble dietary fibre content	582:612	insoluble dietary fibre content	582:612	In particular, the wholemeal spaghetti Cappelli and Core samples recorded the highest protein and insoluble dietary fibre content, respectively.
25666412	7	43	theme	nutritional	1084:1094	arg1	quality					1096:1102	the sensory and nutritional quality	1068:1102	the sensory and nutritional quality	1068:1102	In conclusion, the wholemeal spaghetti Cappelli was found to be an optimum compromise between the sensory and nutritional quality.
25666412	3	44	theme	dietary	592:598	arg1	content					606:612	insoluble dietary fibre content	582:612	insoluble dietary fibre content	582:612	In particular, the wholemeal spaghetti Cappelli and Core samples recorded the highest protein and insoluble dietary fibre content, respectively.
25666412	5	45	theme	colour	842:847	arg1	score					808:812	the low score	800:812	the low score of elasticity, firmness and colour	800:847	Moreover, the wholemeal spaghetti showed the lowest overall quality due to the low score of elasticity, firmness and colour.
25666412	5	46	theme	wholemeal	739:747	arg1	spaghetti					749:757	the wholemeal spaghetti	735:757	the wholemeal spaghetti	735:757	Moreover, the wholemeal spaghetti showed the lowest overall quality due to the low score of elasticity, firmness and colour.
25666412	6	47	theme	wholemeal	868:876	arg1	Cappelli					878:885	the wholemeal Cappelli	864:885	the wholemeal Cappelli	864:885	Specifically, the wholemeal Cappelli recorded a slight rise of the overall quality with respect to other wholemeal samples.
25666412	3	48	theme	Core	536:539	arg1	samples					541:547	Core samples	536:547	Core samples	536:547	In particular, the wholemeal spaghetti Cappelli and Core samples recorded the highest protein and insoluble dietary fibre content, respectively.
25666412	3	49	theme	highest	562:568	arg1	protein					570:576	the highest protein	558:576	the highest protein	558:576	In particular, the wholemeal spaghetti Cappelli and Core samples recorded the highest protein and insoluble dietary fibre content, respectively.
25666412	1	50	from	cultivars	174:182	arg1	semolina					124:131	semolina	124:131	semolina	124:131	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	1	50	from	cultivars	174:182	arg1	flour					147:151	wholemeal flour	137:151	wholemeal flour	137:151	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	1	50	from	cultivars	174:182	arg1	effect					114:119	the effect	110:119	the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality	110:227	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	2	51	theme	chemical	305:312	arg1	composition					314:324	the chemical composition	301:324	the chemical composition	301:324	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	7	52	located	found	1026:1030	arg2	optimum					1041:1047	an optimum compromise	1038:1058	an optimum compromise between the sensory and nutritional quality	1038:1102	In conclusion, the wholemeal spaghetti Cappelli was found to be an optimum compromise between the sensory and nutritional quality.
25666412	7	52	located	found	1026:1030	arg2	Cappelli					1013:1020	the wholemeal spaghetti Cappelli	989:1020	the wholemeal spaghetti Cappelli	989:1020	In conclusion, the wholemeal spaghetti Cappelli was found to be an optimum compromise between the sensory and nutritional quality.
25666412	7	52	located	found	1026:1030	arg1	conclusion					977:986	conclusion	977:986	conclusion	977:986	In conclusion, the wholemeal spaghetti Cappelli was found to be an optimum compromise between the sensory and nutritional quality.
25666412	1	53	theme	pasta	191:195	arg1	cooking					197:203	the pasta cooking	187:203	the pasta cooking	187:203	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	2	54	contain	have	386:389	arg1	they					381:384	they	381:384	they	381:384	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	2	54	contain	have	386:389	arg2	decline					393:399	a decline	391:399	a decline in the cooking quality (high cooking loss)	391:442	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	5	55	theme	lowest	770:775	arg1	quality					785:791	the lowest overall quality	766:791	the lowest overall quality due to the low score of elasticity, firmness and colour	766:847	Moreover, the wholemeal spaghetti showed the lowest overall quality due to the low score of elasticity, firmness and colour.
25666412	2	56	dep	improvement	286:296	arg1	content					368:374	high protein and insoluble dietary fibre content	327:374	content	368:374	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	2	56	dep	improvement	286:296	arg1	protein					332:338	high protein and insoluble dietary fibre content	327:374	protein	332:338	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	4	57	theme	wholemeal	667:675	arg1	Iride					687:691	the wholemeal spaghetti Iride	663:691	the wholemeal spaghetti Iride	663:691	As compared to the other samples, the wholemeal spaghetti Iride recorded a higher cooking loss.
25666412	5	58	theme	overall	777:783	arg1	quality					785:791	the lowest overall quality	766:791	the lowest overall quality due to the low score of elasticity, firmness and colour	766:847	Moreover, the wholemeal spaghetti showed the lowest overall quality due to the low score of elasticity, firmness and colour.
25666412	2	59	theme	insoluble	344:352	arg1	content					368:374	high protein and insoluble dietary fibre content	327:374	content	368:374	The wholemeal spaghetti samples showed an improvement in the chemical composition (high protein and insoluble dietary fibre content) but they have a decline in the cooking quality (high cooking loss) with respect to the semolina spaghetti.
25666412	0	60	theme	nutritional	46:56	arg1	composition					58:68	nutritional composition	46:68	nutritional composition	46:68	Effect of raw material on cooking quality and nutritional composition of durum wheat spaghetti.
25666412	1	61	theme	nutritional	209:219	arg1	quality					221:227	nutritional quality	209:227	nutritional quality	209:227	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	1	62	theme	semolina	124:131	arg1	effect					114:119	the effect	110:119	the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality	110:227	In this study the effect of semolina and wholemeal flour from six durum wheat cultivars on the pasta cooking and nutritional quality was evaluated.
25666412	0	63	theme	wheat	79:83	arg1	spaghetti					85:93	durum wheat spaghetti	73:93	durum wheat spaghetti	73:93	Effect of raw material on cooking quality and nutritional composition of durum wheat spaghetti.
26344277	3	0	from	pH.	445:447	arg1	charged					427:433	charged	427:433	charged	427:433	Zeta potential measurements showed the composite coatings were negative charged at neural pH. XPS results indicated that the CS/CPP composite coatings were covalently bond to the substrate.
26344277	5	1	theme	mRNA	682:685	arg1	expression					687:696	The bone morphogenetic protein-2 (BMP-2) mRNA expression	641:696	The bone morphogenetic protein-2 (BMP-2) mRNA expression	641:696	The bone morphogenetic protein-2 (BMP-2) mRNA expression was significantly up-regulated in MC3T3-E1 cells cultured on the composite coatings and it was twice as much as that of cells cultured on the bare substrate.
26344277	1	2	theme	chitosan/casein	175:189	arg1	coatings					225:232	functional chitosan/casein phospopeptides (CS/CPP) composite coatings	164:232	functional chitosan/casein phospopeptides (CS/CPP) composite coatings	164:232	Using the layer-by-layer deposition method, functional chitosan/casein phospopeptides (CS/CPP) composite coatings were produced on Co-Cr-Mo alloy.
26344277	3	3	theme	Zeta	355:358	arg1	measurements					370:381	Zeta potential measurements	355:381	Zeta potential measurements	355:381	Zeta potential measurements showed the composite coatings were negative charged at neural pH. XPS results indicated that the CS/CPP composite coatings were covalently bond to the substrate.
26344277	6	4	theme	OPG/receptor	918:929	arg1	activator					931:939	OPG/receptor activator	918:939	OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA	918:980	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	1	5	theme	phospopeptides	191:204	arg1	coatings					225:232	functional chitosan/casein phospopeptides (CS/CPP) composite coatings	164:232	functional chitosan/casein phospopeptides (CS/CPP) composite coatings	164:232	Using the layer-by-layer deposition method, functional chitosan/casein phospopeptides (CS/CPP) composite coatings were produced on Co-Cr-Mo alloy.
26344277	3	6	theme	composite	394:402	arg1	coatings					404:411	the composite coatings	390:411	the composite coatings	390:411	Zeta potential measurements showed the composite coatings were negative charged at neural pH. XPS results indicated that the CS/CPP composite coatings were covalently bond to the substrate.
26344277	4	7	theme	MC3T3-E1	550:557	arg1	cells					559:563	MC3T3-E1 cells	550:563	MC3T3-E1 cells	550:563	When MC3T3-E1 cells were seeded on the CS/CPP composite coatings, no cytotoxicity was observed.
26344277	7	8	theme	matrix	1126:1131	arg1	implants					1145:1152	cobalt matrix orthopaedic implants	1119:1152	cobalt matrix orthopaedic implants	1119:1152	These results suggested the CS/CPP composite coatings may have potential application in cobalt matrix orthopaedic implants.
26344277	0	9	theme	orthopedic	99:108	arg1	implants					110:117	orthopedic implants	99:117	orthopedic implants	99:117	Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants.
26344277	6	10	theme	ratio	909:913	arg1	expression					860:869	The expression	856:869	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA	856:980	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	7	11	theme	orthopaedic	1133:1143	arg1	implants					1145:1152	cobalt matrix orthopaedic implants	1119:1152	cobalt matrix orthopaedic implants	1119:1152	These results suggested the CS/CPP composite coatings may have potential application in cobalt matrix orthopaedic implants.
26344277	5	12	theme	bone	645:648	arg1	BMP-2					675:679	BMP-2	675:679	BMP-2	675:679	The bone morphogenetic protein-2 (BMP-2) mRNA expression was significantly up-regulated in MC3T3-E1 cells cultured on the composite coatings and it was twice as much as that of cells cultured on the bare substrate.
26344277	5	12	theme	bone	645:648	arg1	protein-2					664:672	bone morphogenetic protein-2	645:672	The bone morphogenetic protein-2 (BMP-2) mRNA expression	641:696	The bone morphogenetic protein-2 (BMP-2) mRNA expression was significantly up-regulated in MC3T3-E1 cells cultured on the composite coatings and it was twice as much as that of cells cultured on the bare substrate.
26344277	2	13	contain	had	297:299	arg1	hydrophilic					342:352	hydrophilic	342:352	hydrophilic	342:352	The CS/CPP composite coatings had the dendritic topography, and were quite hydrophilic.
26344277	2	13	contain	had	297:299	arg1	coatings					288:295	The CS/CPP composite coatings	267:295	The CS/CPP composite coatings	267:295	The CS/CPP composite coatings had the dendritic topography, and were quite hydrophilic.
26344277	2	13	contain	had	297:299	arg2	topography					315:324	the dendritic topography	301:324	the dendritic topography	301:324	The CS/CPP composite coatings had the dendritic topography, and were quite hydrophilic.
26344277	5	14	theme	protein-2	664:672	arg1	expression					687:696	The bone morphogenetic protein-2 (BMP-2) mRNA expression	641:696	The bone morphogenetic protein-2 (BMP-2) mRNA expression	641:696	The bone morphogenetic protein-2 (BMP-2) mRNA expression was significantly up-regulated in MC3T3-E1 cells cultured on the composite coatings and it was twice as much as that of cells cultured on the bare substrate.
26344277	5	15	theme	morphogenetic	650:662	arg1	BMP-2					675:679	BMP-2	675:679	BMP-2	675:679	The bone morphogenetic protein-2 (BMP-2) mRNA expression was significantly up-regulated in MC3T3-E1 cells cultured on the composite coatings and it was twice as much as that of cells cultured on the bare substrate.
26344277	5	15	theme	morphogenetic	650:662	arg1	protein-2					664:672	bone morphogenetic protein-2	645:672	The bone morphogenetic protein-2 (BMP-2) mRNA expression	641:696	The bone morphogenetic protein-2 (BMP-2) mRNA expression was significantly up-regulated in MC3T3-E1 cells cultured on the composite coatings and it was twice as much as that of cells cultured on the bare substrate.
26344277	2	16	theme	dendritic	305:313	arg1	topography					315:324	the dendritic topography	301:324	the dendritic topography	301:324	The CS/CPP composite coatings had the dendritic topography, and were quite hydrophilic.
26344277	7	17	theme	cobalt	1119:1124	arg1	implants					1145:1152	cobalt matrix orthopaedic implants	1119:1152	cobalt matrix orthopaedic implants	1119:1152	These results suggested the CS/CPP composite coatings may have potential application in cobalt matrix orthopaedic implants.
26344277	1	18	theme	CS/CPP	207:212	arg1	coatings					225:232	functional chitosan/casein phospopeptides (CS/CPP) composite coatings	164:232	functional chitosan/casein phospopeptides (CS/CPP) composite coatings	164:232	Using the layer-by-layer deposition method, functional chitosan/casein phospopeptides (CS/CPP) composite coatings were produced on Co-Cr-Mo alloy.
26344277	3	19	theme	charged	427:433	arg1	XPS					449:451	negative charged at neural pH. XPS	418:451	negative charged at neural pH. XPS	418:451	Zeta potential measurements showed the composite coatings were negative charged at neural pH. XPS results indicated that the CS/CPP composite coatings were covalently bond to the substrate.
26344277	6	20	theme	mRNA	896:899	arg1	expression					860:869	The expression	856:869	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA	856:980	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	7	21	theme	potential	1094:1102	arg1	application					1104:1114	potential application	1094:1114	potential application	1094:1114	These results suggested the CS/CPP composite coatings may have potential application in cobalt matrix orthopaedic implants.
26344277	0	22	theme	composite	76:84	arg1	coatings					86:93	composite coatings	76:93	composite coatings	76:93	Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants.
26344277	4	23	theme	composite	591:599	arg1	coatings					601:608	the CS/CPP composite coatings	580:608	the CS/CPP composite coatings	580:608	When MC3T3-E1 cells were seeded on the CS/CPP composite coatings, no cytotoxicity was observed.
26344277	1	24	theme	composite	215:223	arg1	coatings					225:232	functional chitosan/casein phospopeptides (CS/CPP) composite coatings	164:232	functional chitosan/casein phospopeptides (CS/CPP) composite coatings	164:232	Using the layer-by-layer deposition method, functional chitosan/casein phospopeptides (CS/CPP) composite coatings were produced on Co-Cr-Mo alloy.
26344277	5	25	theme	bare	840:843	arg1	substrate					845:853	the bare substrate	836:853	the bare substrate	836:853	The bone morphogenetic protein-2 (BMP-2) mRNA expression was significantly up-regulated in MC3T3-E1 cells cultured on the composite coatings and it was twice as much as that of cells cultured on the bare substrate.
26344277	6	26	theme	mRNA	977:980	arg1	activator					931:939	OPG/receptor activator	918:939	OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA	918:980	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	3	27	theme	negative	418:425	arg1	XPS					449:451	negative charged at neural pH. XPS	418:451	negative charged at neural pH. XPS	418:451	Zeta potential measurements showed the composite coatings were negative charged at neural pH. XPS results indicated that the CS/CPP composite coatings were covalently bond to the substrate.
26344277	6	28	theme	activator	931:939	arg1	mRNA					896:899	osteoprotegerin (OPG) mRNA	874:899	osteoprotegerin (OPG) mRNA	874:899	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	6	28	theme	activator	931:939	arg1	ratio					909:913	the ratio	905:913	the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA	905:980	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	0	29	theme	chitosan	40:47	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants.
26344277	0	29	theme	chitosan	40:47	arg1	properties					26:35	bioactive properties	16:35	bioactive properties	16:35	Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants.
26344277	7	30	theme	composite	1066:1074	arg1	coatings					1076:1083	the CS/CPP composite coatings	1055:1083	the CS/CPP composite coatings	1055:1083	These results suggested the CS/CPP composite coatings may have potential application in cobalt matrix orthopaedic implants.
26344277	3	31	theme	composite	487:495	arg1	coatings					497:504	the CS/CPP composite coatings	476:504	the CS/CPP composite coatings	476:504	Zeta potential measurements showed the composite coatings were negative charged at neural pH. XPS results indicated that the CS/CPP composite coatings were covalently bond to the substrate.
26344277	3	31	theme	composite	487:495	arg1	bond					522:525	bond	522:525	bond to the substrate	522:542	Zeta potential measurements showed the composite coatings were negative charged at neural pH. XPS results indicated that the CS/CPP composite coatings were covalently bond to the substrate.
26344277	5	32	theme	composite	763:771	arg1	coatings					773:780	the composite coatings	759:780	the composite coatings	759:780	The bone morphogenetic protein-2 (BMP-2) mRNA expression was significantly up-regulated in MC3T3-E1 cells cultured on the composite coatings and it was twice as much as that of cells cultured on the bare substrate.
26344277	0	33	theme	casein	53:58	arg1	phosphopeptides					60:74	casein phosphopeptides	53:74	casein phosphopeptides	53:74	Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants.
26344277	6	34	theme	ligand	962:967	arg1	mRNA					977:980	nuclear factor-κB ligand (RNAKL) mRNA	944:980	nuclear factor-κB ligand (RNAKL) mRNA	944:980	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	3	35	dep	showed	383:388	arg1	XPS					449:451	negative charged at neural pH. XPS	418:451	negative charged at neural pH. XPS	418:451	Zeta potential measurements showed the composite coatings were negative charged at neural pH. XPS results indicated that the CS/CPP composite coatings were covalently bond to the substrate.
26344277	6	36	theme	osteoprotegerin	874:888	arg1	mRNA					896:899	osteoprotegerin (OPG) mRNA	874:899	osteoprotegerin (OPG) mRNA	874:899	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	0	37	dep	chitosan	40:47	arg1	coatings					86:93	composite coatings	76:93	composite coatings	76:93	Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants.
26344277	6	38	theme	factor-κB	952:960	arg1	ligand					962:967	nuclear factor-κB ligand	944:967	nuclear factor-κB ligand (RNAKL) mRNA	944:980	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	6	38	theme	factor-κB	952:960	arg1	RNAKL					970:974	RNAKL	970:974	RNAKL	970:974	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	1	39	theme	layer-by-layer	130:143	arg1	method					156:161	the layer-by-layer deposition method	126:161	the layer-by-layer deposition method	126:161	Using the layer-by-layer deposition method, functional chitosan/casein phospopeptides (CS/CPP) composite coatings were produced on Co-Cr-Mo alloy.
26344277	6	40	theme	nuclear	944:950	arg1	ligand					962:967	nuclear factor-κB ligand	944:967	nuclear factor-κB ligand (RNAKL) mRNA	944:980	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	6	40	theme	nuclear	944:950	arg1	RNAKL					970:974	RNAKL	970:974	RNAKL	970:974	The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively.
26344277	5	41	theme	MC3T3-E1	732:739	arg1	cells					741:745	MC3T3-E1 cells	732:745	MC3T3-E1 cells cultured on the composite coatings	732:780	The bone morphogenetic protein-2 (BMP-2) mRNA expression was significantly up-regulated in MC3T3-E1 cells cultured on the composite coatings and it was twice as much as that of cells cultured on the bare substrate.
26344277	1	42	theme	deposition	145:154	arg1	method					156:161	the layer-by-layer deposition method	126:161	the layer-by-layer deposition method	126:161	Using the layer-by-layer deposition method, functional chitosan/casein phospopeptides (CS/CPP) composite coatings were produced on Co-Cr-Mo alloy.
26344277	1	43	theme	Co-Cr-Mo	251:258	arg1	alloy					260:264	Co-Cr-Mo alloy	251:264	Co-Cr-Mo alloy	251:264	Using the layer-by-layer deposition method, functional chitosan/casein phospopeptides (CS/CPP) composite coatings were produced on Co-Cr-Mo alloy.
26344277	3	44	theme	potential	360:368	arg1	measurements					370:381	Zeta potential measurements	355:381	Zeta potential measurements	355:381	Zeta potential measurements showed the composite coatings were negative charged at neural pH. XPS results indicated that the CS/CPP composite coatings were covalently bond to the substrate.
26344277	2	45	theme	composite	278:286	arg1	coatings					288:295	The CS/CPP composite coatings	267:295	The CS/CPP composite coatings	267:295	The CS/CPP composite coatings had the dendritic topography, and were quite hydrophilic.
26344277	2	45	theme	composite	278:286	arg1	hydrophilic					342:352	hydrophilic	342:352	hydrophilic	342:352	The CS/CPP composite coatings had the dendritic topography, and were quite hydrophilic.
26344277	0	46	theme	phosphopeptides	60:74	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants.
26344277	0	46	theme	phosphopeptides	60:74	arg1	properties					26:35	bioactive properties	16:35	bioactive properties	16:35	Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants.
26344277	0	47	theme	bioactive	16:24	arg1	properties					26:35	bioactive properties	16:35	bioactive properties	16:35	Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants.
26344277	1	48	theme	functional	164:173	arg1	coatings					225:232	functional chitosan/casein phospopeptides (CS/CPP) composite coatings	164:232	functional chitosan/casein phospopeptides (CS/CPP) composite coatings	164:232	Using the layer-by-layer deposition method, functional chitosan/casein phospopeptides (CS/CPP) composite coatings were produced on Co-Cr-Mo alloy.
26344277	7	49	dep	suggested	1045:1053	arg1	have					1089:1092	have	1089:1092	suggested the CS/CPP composite coatings may have potential application in cobalt matrix orthopaedic implants	1045:1152	These results suggested the CS/CPP composite coatings may have potential application in cobalt matrix orthopaedic implants.
27199227	10	0	dep	feature	1437:1443	arg1	C18 					1459:1462	C18 	1459:1462	C18 	1459:1462	The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c).
27199227	12	1	theme	novel	1560:1564	arg1	species					1566:1572	this novel species	1555:1572	this novel species of the genus Vibrio	1555:1592	The name proposed for this novel species of the genus Vibrio is Vibrio algivorus sp.
27199227	8	2	theme	strain	1135:1140	arg1	SA2T					1142:1145	strain SA2T	1135:1145	strain SA2T	1135:1145	These results suggested that strain SA2T represented a novel species within the genus Vibrio.
27199227	13	3	theme	type	1633:1636	arg1	strain					1638:1643	the type strain	1629:1643	the type strain	1629:1643	nov., with the type strain designated SA2T (=DSM 29824T=NBRC 111146T).
27199227	6	4	theme	carbon	885:890	arg1	source					892:897	a sole carbon source	878:897	a sole carbon source	878:897	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	6	4	theme	carbon	885:890	arg1	agarose					867:873	agarose	867:873	agarose	867:873	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	10	5	dep	C16 	1386:1389	arg1	C16 					1404:1407	C16 	1404:1407	C16 	1404:1407	The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c).
27199227	10	5	dep	C16 	1386:1389	arg1	 1ω6c					1391:1395	 1ω6c	1391:1395	 1ω6c	1391:1395	The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c).
27199227	10	5	dep	C16 	1386:1389	arg1	 1ω7c					1409:1413	 1ω7c	1409:1413	 1ω7c	1409:1413	The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c).
27199227	10	6	theme	fatty	1339:1343	arg1	acids					1345:1349	The major fatty acids	1329:1349	The major fatty acids	1329:1349	The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c).
27199227	4	7	theme	rumoiensis	612:621	arg1	SA2T					579:582	strain SA2T	572:582	strain SA2T	572:582	This suggested that strain SA2T could be a subspecies of V. rumoiensis or V. litoralis.
27199227	4	7	theme	rumoiensis	612:621	arg1	subspecies					595:604	a subspecies	593:604	a subspecies of V. rumoiensis or V. litoralis	593:637	This suggested that strain SA2T could be a subspecies of V. rumoiensis or V. litoralis.
27199227	2	8	dep	agarose-	138:145	arg1	rod-shaped					210:219	rod-shaped	210:219	rod-shaped	210:219	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	2	8	dep	agarose-	138:145	arg1	non-motile					198:207	non-motile	198:207	non-motile	198:207	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	2	8	dep	agarose-	138:145	arg1	Gram-reaction-negative					174:195	Gram-reaction-negative	174:195	Gram-reaction-negative	174:195	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	7	9	theme	ZYM	1018:1020	arg1	kits					1022:1025	API 20NE and API ZYM kits	1001:1025	API 20NE and API ZYM kits	1001:1025	Furthermore, results using API 20NE and API ZYM kits indicated that their enzymic and physiological phenotypes were also different.
27199227	5	10	theme	DNA-DNA	649:655	arg1	hybridization					657:669	DNA-DNA hybridization	649:669	DNA-DNA hybridization results	649:677	However, DNA-DNA hybridization results showed only 37.5 % relatedness to DSM 19141T and 44.7 % relatedness to DSM 17657T, which was far lower than the 70 % widely accepted to define common species.
27199227	1	11	theme	agarose-assimilating	44:63	arg1	bacterium					65:73	an alginate- and agarose-assimilating bacterium	27:73	an alginate- and agarose-assimilating bacterium	27:73	nov., an alginate- and agarose-assimilating bacterium isolated from the gut flora of a turban shell marine snail.
27199227	1	11	theme	agarose-assimilating	44:63	arg1	nov.					21:24	nov.	21:24	nov.	21:24	nov., an alginate- and agarose-assimilating bacterium isolated from the gut flora of a turban shell marine snail.
27199227	1	12	theme	shell	115:119	arg1	snail					128:132	a turban shell marine snail	106:132	a turban shell marine snail	106:132	nov., an alginate- and agarose-assimilating bacterium isolated from the gut flora of a turban shell marine snail.
27199227	5	13	theme	hybridization	657:669	arg1	results					671:677	DNA-DNA hybridization results	649:677	DNA-DNA hybridization results	649:677	However, DNA-DNA hybridization results showed only 37.5 % relatedness to DSM 19141T and 44.7 % relatedness to DSM 17657T, which was far lower than the 70 % widely accepted to define common species.
27199227	8	14	theme	novel	1161:1165	arg1	species					1167:1173	a novel species	1159:1173	a novel species within the genus Vibrio	1159:1197	These results suggested that strain SA2T represented a novel species within the genus Vibrio.
27199227	5	15	theme	DSM	750:752	arg1	17657T					754:759	DSM 17657T	750:759	DSM 17657T	750:759	However, DNA-DNA hybridization results showed only 37.5 % relatedness to DSM 19141T and 44.7 % relatedness to DSM 17657T, which was far lower than the 70 % widely accepted to define common species.
27199227	3	16	theme	rumoiensis	468:477	arg1	19141T					483:488	Vibrio rumoiensis DSM 19141T	461:488	Vibrio rumoiensis DSM 19141T	461:488	The 16S rRNA gene sequence of strain SA2T was 99.59 % identical to that of Vibrio rumoiensis DSM 19141T and 98.19 % identical to that of Vibrio litoralis DSM 17657T.
27199227	3	17	theme	rRNA	394:397	arg1	sequence					404:411	The 16S rRNA gene sequence	386:411	The 16S rRNA gene sequence of strain SA2T	386:426	The 16S rRNA gene sequence of strain SA2T was 99.59 % identical to that of Vibrio rumoiensis DSM 19141T and 98.19 % identical to that of Vibrio litoralis DSM 17657T.
27199227	7	18	theme	enzymic	1048:1054	arg1	phenotypes					1074:1083	their enzymic and physiological phenotypes	1042:1083	their enzymic and physiological phenotypes	1042:1083	Furthermore, results using API 20NE and API ZYM kits indicated that their enzymic and physiological phenotypes were also different.
27199227	6	19	dep	strains	908:914	arg1	19141T					920:925	DSM 19141T	916:925	DSM 19141T	916:925	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	6	19	dep	strains	908:914	arg1	17657T					935:940	DSM 17657T	931:940	DSM 17657T	931:940	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	6	19	dep	strains	908:914	arg1	strains					908:914	strains	908:914	strains DSM 19141T and DSM 17657T	908:940	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	9	20	theme	polar	1260:1264	arg1	lipids					1266:1271	its major polar lipids	1250:1271	its major polar lipids	1250:1271	The major isoprenoid quinone in SA2T was Q-8, and its major polar lipids were phosphatidylethanolamine and phosphatidylglycerol.
27199227	3	21	theme	DSM	479:481	arg1	19141T					483:488	Vibrio rumoiensis DSM 19141T	461:488	Vibrio rumoiensis DSM 19141T	461:488	The 16S rRNA gene sequence of strain SA2T was 99.59 % identical to that of Vibrio rumoiensis DSM 19141T and 98.19 % identical to that of Vibrio litoralis DSM 17657T.
27199227	3	22	theme	gene	399:402	arg1	sequence					404:411	The 16S rRNA gene sequence	386:411	The 16S rRNA gene sequence of strain SA2T	386:426	The 16S rRNA gene sequence of strain SA2T was 99.59 % identical to that of Vibrio rumoiensis DSM 19141T and 98.19 % identical to that of Vibrio litoralis DSM 17657T.
27199227	0	23	theme	algivorus	7:15	arg1	sp					17:18	Vibrio algivorus sp	0:18	Vibrio algivorus sp.	0:19	Vibrio algivorus sp.
27199227	3	24	theme	Vibrio	461:466	arg1	19141T					483:488	Vibrio rumoiensis DSM 19141T	461:488	Vibrio rumoiensis DSM 19141T	461:488	The 16S rRNA gene sequence of strain SA2T was 99.59 % identical to that of Vibrio rumoiensis DSM 19141T and 98.19 % identical to that of Vibrio litoralis DSM 17657T.
27199227	5	25	dep	relatedness	698:708	arg1	%					696:696	%	696:696	%	696:696	However, DNA-DNA hybridization results showed only 37.5 % relatedness to DSM 19141T and 44.7 % relatedness to DSM 17657T, which was far lower than the 70 % widely accepted to define common species.
27199227	2	26	theme	shell	294:298	arg1	Turbo					311:315	Turbo	311:315	Turbo	311:315	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	2	26	theme	shell	294:298	arg1	snail					304:308	a turban shell sea snail	285:308	a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan	285:383	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	0	27	theme	Vibrio	0:5	arg1	sp					17:18	Vibrio algivorus sp	0:18	Vibrio algivorus sp.	0:19	Vibrio algivorus sp.
27199227	10	28	theme	summed	1430:1435	arg1	feature					1437:1443	summed feature 8	1430:1445	summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c)	1430:1487	The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c).
27199227	6	29	dep	assimilate	856:865	arg1	whereas					900:906	whereas	900:906	whereas	900:906	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	2	30	theme	turban	287:292	arg1	Turbo					311:315	Turbo	311:315	Turbo	311:315	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	2	30	theme	turban	287:292	arg1	snail					304:308	a turban shell sea snail	285:308	a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan	285:383	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	2	31	theme	Noto	342:345	arg1	Japan					379:383	Japan	379:383	Japan	379:383	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	2	31	theme	Noto	342:345	arg1	Peninsula					347:355	Noto Peninsula	342:355	Noto Peninsula	342:355	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	5	32	theme	%	733:733	arg1	relatedness					735:745	44.7 % relatedness	728:745	44.7 % relatedness to DSM 17657T, which was far lower than the 70 % widely accepted to define common species	728:835	However, DNA-DNA hybridization results showed only 37.5 % relatedness to DSM 19141T and 44.7 % relatedness to DSM 17657T, which was far lower than the 70 % widely accepted to define common species.
27199227	5	33	theme	44.7 	728:732	arg1	%					733:733	%	733:733	%	733:733	However, DNA-DNA hybridization results showed only 37.5 % relatedness to DSM 19141T and 44.7 % relatedness to DSM 17657T, which was far lower than the 70 % widely accepted to define common species.
27199227	11	34	theme	G+C	1498:1500	arg1	content					1502:1508	The DNA G+C content	1490:1508	The DNA G+C content of SA2T	1490:1516	The DNA G+C content of SA2T was 40.7 mol%.
27199227	11	34	theme	G+C	1498:1500	arg1	%					1530:1530	40.7 mol%	1522:1530	40.7 mol%	1522:1530	The DNA G+C content of SA2T was 40.7 mol%.
27199227	2	35	dep	Turbo	311:315	arg1	cornutus					317:324	Turbo cornutus	311:324	Turbo cornutus	311:324	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	10	36	theme	major	1333:1337	arg1	acids					1345:1349	The major fatty acids	1329:1349	The major fatty acids	1329:1349	The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c).
27199227	3	37	theme	strain	416:421	arg1	SA2T					423:426	strain SA2T	416:426	strain SA2T	416:426	The 16S rRNA gene sequence of strain SA2T was 99.59 % identical to that of Vibrio rumoiensis DSM 19141T and 98.19 % identical to that of Vibrio litoralis DSM 17657T.
27199227	9	38	theme	isoprenoid	1210:1219	arg1	quinone					1221:1227	The major isoprenoid quinone	1200:1227	The major isoprenoid quinone in SA2T	1200:1235	The major isoprenoid quinone in SA2T was Q-8, and its major polar lipids were phosphatidylethanolamine and phosphatidylglycerol.
27199227	9	38	theme	isoprenoid	1210:1219	arg1	Q-8					1241:1243	Q-8	1241:1243	Q-8	1241:1243	The major isoprenoid quinone in SA2T was Q-8, and its major polar lipids were phosphatidylethanolamine and phosphatidylglycerol.
27199227	5	39	dep	%	696:696	arg1	37.5 					691:695	37.5 	691:695	37.5 	691:695	However, DNA-DNA hybridization results showed only 37.5 % relatedness to DSM 19141T and 44.7 % relatedness to DSM 17657T, which was far lower than the 70 % widely accepted to define common species.
27199227	3	40	theme	SA2T	423:426	arg1	sequence					404:411	The 16S rRNA gene sequence	386:411	The 16S rRNA gene sequence of strain SA2T	386:426	The 16S rRNA gene sequence of strain SA2T was 99.59 % identical to that of Vibrio rumoiensis DSM 19141T and 98.19 % identical to that of Vibrio litoralis DSM 17657T.
27199227	13	41	with	nov.	1618:1621	arg1	strain					1638:1643	the type strain	1629:1643	the type strain	1629:1643	nov., with the type strain designated SA2T (=DSM 29824T=NBRC 111146T).
27199227	11	42	theme	DNA	1494:1496	arg1	content					1502:1508	The DNA G+C content	1490:1508	The DNA G+C content of SA2T	1490:1516	The DNA G+C content of SA2T was 40.7 mol%.
27199227	11	42	theme	DNA	1494:1496	arg1	%					1530:1530	40.7 mol%	1522:1530	40.7 mol%	1522:1530	The DNA G+C content of SA2T was 40.7 mol%.
27199227	2	43	theme	alginate-assimilating	151:171	arg1	bacterium					221:229	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium	135:229	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium	135:229	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	3	44	theme	Vibrio	523:528	arg1	DSM					540:542	Vibrio litoralis DSM 17657T	523:549	Vibrio litoralis DSM 17657T	523:549	The 16S rRNA gene sequence of strain SA2T was 99.59 % identical to that of Vibrio rumoiensis DSM 19141T and 98.19 % identical to that of Vibrio litoralis DSM 17657T.
27199227	3	45	theme	16S	390:392	arg1	rRNA					394:397	The 16S rRNA	386:397	The 16S rRNA gene sequence of strain SA2T	386:426	The 16S rRNA gene sequence of strain SA2T was 99.59 % identical to that of Vibrio rumoiensis DSM 19141T and 98.19 % identical to that of Vibrio litoralis DSM 17657T.
27199227	9	46	theme	major	1204:1208	arg1	quinone					1221:1227	The major isoprenoid quinone	1200:1227	The major isoprenoid quinone in SA2T	1200:1235	The major isoprenoid quinone in SA2T was Q-8, and its major polar lipids were phosphatidylethanolamine and phosphatidylglycerol.
27199227	9	46	theme	major	1204:1208	arg1	Q-8					1241:1243	Q-8	1241:1243	Q-8	1241:1243	The major isoprenoid quinone in SA2T was Q-8, and its major polar lipids were phosphatidylethanolamine and phosphatidylglycerol.
27199227	7	47	theme	API	1014:1016	arg1	ZYM					1018:1020	API ZYM	1014:1020	API ZYM	1014:1020	Furthermore, results using API 20NE and API ZYM kits indicated that their enzymic and physiological phenotypes were also different.
27199227	6	48	theme	Strain	838:843	arg1	SA2T					845:848	Strain SA2T	838:848	Strain SA2T	838:848	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	6	49	theme	sole	880:883	arg1	source					892:897	a sole carbon source	878:897	a sole carbon source	878:897	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	6	49	theme	sole	880:883	arg1	agarose					867:873	agarose	867:873	agarose	867:873	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	3	50	theme	litoralis	530:538	arg1	DSM					540:542	Vibrio litoralis DSM 17657T	523:549	Vibrio litoralis DSM 17657T	523:549	The 16S rRNA gene sequence of strain SA2T was 99.59 % identical to that of Vibrio rumoiensis DSM 19141T and 98.19 % identical to that of Vibrio litoralis DSM 17657T.
27199227	4	51	theme	strain	572:577	arg1	SA2T					579:582	strain SA2T	572:582	strain SA2T	572:582	This suggested that strain SA2T could be a subspecies of V. rumoiensis or V. litoralis.
27199227	4	51	theme	strain	572:577	arg1	subspecies					595:604	a subspecies	593:604	a subspecies of V. rumoiensis or V. litoralis	593:637	This suggested that strain SA2T could be a subspecies of V. rumoiensis or V. litoralis.
27199227	5	52	theme	DSM	713:715	arg1	19141T					717:722	DSM 19141T	713:722	DSM 19141T	713:722	However, DNA-DNA hybridization results showed only 37.5 % relatedness to DSM 19141T and 44.7 % relatedness to DSM 17657T, which was far lower than the 70 % widely accepted to define common species.
27199227	12	53	theme	Vibrio	1597:1602	arg1	sp					1614:1615	Vibrio algivorus sp	1597:1615	Vibrio algivorus sp	1597:1615	The name proposed for this novel species of the genus Vibrio is Vibrio algivorus sp.
27199227	12	53	theme	Vibrio	1597:1602	arg1	name					1537:1540	The name	1533:1540	The name proposed for this novel species of the genus Vibrio	1533:1592	The name proposed for this novel species of the genus Vibrio is Vibrio algivorus sp.
27199227	2	54	theme	agarose-	138:145	arg1	bacterium					221:229	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium	135:229	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium	135:229	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	4	55	theme	litoralis	629:637	arg1	SA2T					579:582	strain SA2T	572:582	strain SA2T	572:582	This suggested that strain SA2T could be a subspecies of V. rumoiensis or V. litoralis.
27199227	4	55	theme	litoralis	629:637	arg1	subspecies					595:604	a subspecies	593:604	a subspecies of V. rumoiensis or V. litoralis	593:637	This suggested that strain SA2T could be a subspecies of V. rumoiensis or V. litoralis.
27199227	7	56	theme	20NE	1005:1008	arg1	kits					1022:1025	API 20NE and API ZYM kits	1001:1025	API 20NE and API ZYM kits	1001:1025	Furthermore, results using API 20NE and API ZYM kits indicated that their enzymic and physiological phenotypes were also different.
27199227	2	57	attach	isolated	260:267	arg1	gut					278:280	the gut	274:280	the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan	274:383	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	2	57	attach	isolated	260:267	arg2	bacterium					221:229	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium	135:229	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium	135:229	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	9	58	from	quinone	1221:1227	arg1	SA2T					1232:1235	SA2T	1232:1235	SA2T	1232:1235	The major isoprenoid quinone in SA2T was Q-8, and its major polar lipids were phosphatidylethanolamine and phosphatidylglycerol.
27199227	12	59	theme	Vibrio	1587:1592	arg1	species					1566:1572	this novel species	1555:1572	this novel species of the genus Vibrio	1555:1592	The name proposed for this novel species of the genus Vibrio is Vibrio algivorus sp.
27199227	1	60	theme	alginate-	30:38	arg1	bacterium					65:73	an alginate- and agarose-assimilating bacterium	27:73	an alginate- and agarose-assimilating bacterium	27:73	nov., an alginate- and agarose-assimilating bacterium isolated from the gut flora of a turban shell marine snail.
27199227	1	60	theme	alginate-	30:38	arg1	nov.					21:24	nov.	21:24	nov.	21:24	nov., an alginate- and agarose-assimilating bacterium isolated from the gut flora of a turban shell marine snail.
27199227	5	61	theme	common	822:827	arg1	species					829:835	common species	822:835	common species	822:835	However, DNA-DNA hybridization results showed only 37.5 % relatedness to DSM 19141T and 44.7 % relatedness to DSM 17657T, which was far lower than the 70 % widely accepted to define common species.
27199227	11	62	theme	SA2T	1513:1516	arg1	content					1502:1508	The DNA G+C content	1490:1508	The DNA G+C content of SA2T	1490:1516	The DNA G+C content of SA2T was 40.7 mol%.
27199227	11	62	theme	SA2T	1513:1516	arg1	%					1530:1530	40.7 mol%	1522:1530	40.7 mol%	1522:1530	The DNA G+C content of SA2T was 40.7 mol%.
27199227	10	63	dep	C18 	1459:1462	arg1	C18 					1477:1480	C18 	1477:1480	C18 	1477:1480	The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c).
27199227	10	63	dep	C18 	1459:1462	arg1	 1ω6c					1464:1468	 1ω6c	1464:1468	 1ω6c	1464:1468	The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c).
27199227	10	63	dep	C18 	1459:1462	arg1	 1ω7c					1482:1486	 1ω7c	1482:1486	 1ω7c	1482:1486	The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c).
27199227	12	64	theme	genus	1581:1585	arg1	Vibrio					1587:1592	the genus Vibrio	1577:1592	the genus Vibrio	1577:1592	The name proposed for this novel species of the genus Vibrio is Vibrio algivorus sp.
27199227	1	65	theme	turban	108:113	arg1	snail					128:132	a turban shell marine snail	106:132	a turban shell marine snail	106:132	nov., an alginate- and agarose-assimilating bacterium isolated from the gut flora of a turban shell marine snail.
27199227	1	66	theme	gut	93:95	arg1	flora					97:101	the gut flora	89:101	the gut flora of a turban shell marine snail	89:132	nov., an alginate- and agarose-assimilating bacterium isolated from the gut flora of a turban shell marine snail.
27199227	11	67	theme	mol	1527:1529	arg1	content					1502:1508	The DNA G+C content	1490:1508	The DNA G+C content of SA2T	1490:1516	The DNA G+C content of SA2T was 40.7 mol%.
27199227	11	67	theme	mol	1527:1529	arg1	%					1530:1530	40.7 mol%	1522:1530	40.7 mol%	1522:1530	The DNA G+C content of SA2T was 40.7 mol%.
27199227	12	68	theme	algivorus	1604:1612	arg1	sp					1614:1615	Vibrio algivorus sp	1597:1615	Vibrio algivorus sp	1597:1615	The name proposed for this novel species of the genus Vibrio is Vibrio algivorus sp.
27199227	12	68	theme	algivorus	1604:1612	arg1	name					1537:1540	The name	1533:1540	The name proposed for this novel species of the genus Vibrio	1533:1592	The name proposed for this novel species of the genus Vibrio is Vibrio algivorus sp.
27199227	11	69	theme	40.7	1522:1525	arg1	content					1502:1508	The DNA G+C content	1490:1508	The DNA G+C content of SA2T	1490:1516	The DNA G+C content of SA2T was 40.7 mol%.
27199227	11	69	theme	40.7	1522:1525	arg1	%					1530:1530	40.7 mol%	1522:1530	40.7 mol%	1522:1530	The DNA G+C content of SA2T was 40.7 mol%.
27199227	8	70	theme	genus	1186:1190	arg1	Vibrio					1192:1197	the genus Vibrio	1182:1197	the genus Vibrio	1182:1197	These results suggested that strain SA2T represented a novel species within the genus Vibrio.
27199227	2	71	theme	sea	300:302	arg1	Turbo					311:315	Turbo	311:315	Turbo	311:315	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	2	71	theme	sea	300:302	arg1	snail					304:308	a turban shell sea snail	285:308	a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan	285:383	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	13	72	theme	=DSM	1662:1665	arg1	111146T					1679:1685	=DSM 29824T=NBRC 111146T	1662:1685	=DSM 29824T=NBRC 111146T	1662:1685	nov., with the type strain designated SA2T (=DSM 29824T=NBRC 111146T).
27199227	13	72	theme	=DSM	1662:1665	arg1	SA2T					1656:1659	SA2T	1656:1659	SA2T (=DSM 29824T=NBRC 111146T)	1656:1686	nov., with the type strain designated SA2T (=DSM 29824T=NBRC 111146T).
27199227	2	73	theme	snail	304:308	arg1	gut					278:280	the gut	274:280	the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan	274:383	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	6	74	theme	DSM	916:918	arg1	19141T					920:925	DSM 19141T	916:925	DSM 19141T	916:925	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	6	74	theme	DSM	916:918	arg1	strains					908:914	strains	908:914	strains DSM 19141T and DSM 17657T	908:940	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	6	75	theme	DSM	931:933	arg1	17657T					935:940	DSM 17657T	931:940	DSM 17657T	931:940	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	6	75	theme	DSM	931:933	arg1	strains					908:914	strains	908:914	strains DSM 19141T and DSM 17657T	908:940	Strain SA2T could assimilate agarose as a sole carbon source, whereas strains DSM 19141T and DSM 17657T could not assimilate it at all.
27199227	1	76	theme	marine	121:126	arg1	snail					128:132	a turban shell marine snail	106:132	a turban shell marine snail	106:132	nov., an alginate- and agarose-assimilating bacterium isolated from the gut flora of a turban shell marine snail.
27199227	13	77	theme	29824T=NBRC	1667:1677	arg1	111146T					1679:1685	=DSM 29824T=NBRC 111146T	1662:1685	=DSM 29824T=NBRC 111146T	1662:1685	nov., with the type strain designated SA2T (=DSM 29824T=NBRC 111146T).
27199227	13	77	theme	29824T=NBRC	1667:1677	arg1	SA2T					1656:1659	SA2T	1656:1659	SA2T (=DSM 29824T=NBRC 111146T)	1656:1686	nov., with the type strain designated SA2T (=DSM 29824T=NBRC 111146T).
27199227	7	78	theme	API	1001:1003	arg1	20NE					1005:1008	API 20NE	1001:1008	API 20NE	1001:1008	Furthermore, results using API 20NE and API ZYM kits indicated that their enzymic and physiological phenotypes were also different.
27199227	2	79	theme	strain	243:248	arg1	SA2T					250:253	strain SA2T	243:253	strain SA2T	243:253	An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2T, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan.
27199227	1	80	theme	snail	128:132	arg1	flora					97:101	the gut flora	89:101	the gut flora of a turban shell marine snail	89:132	nov., an alginate- and agarose-assimilating bacterium isolated from the gut flora of a turban shell marine snail.
27199227	9	81	theme	major	1254:1258	arg1	lipids					1266:1271	its major polar lipids	1250:1271	its major polar lipids	1250:1271	The major isoprenoid quinone in SA2T was Q-8, and its major polar lipids were phosphatidylethanolamine and phosphatidylglycerol.
27199227	7	82	theme	physiological	1060:1072	arg1	phenotypes					1074:1083	their enzymic and physiological phenotypes	1042:1083	their enzymic and physiological phenotypes	1042:1083	Furthermore, results using API 20NE and API ZYM kits indicated that their enzymic and physiological phenotypes were also different.
27156697	5	0	theme	SF	698:699	arg1	conformation					682:693	conformation	682:693	conformation of SF from random coil	682:716	FTIR results indicated a change of conformation of SF from random coil to β-sheet after hydrogel formation.
27156697	11	1	theme	biomedical	1564:1573	arg1	applications					1575:1586	the biomedical applications	1560:1586	the biomedical applications in tissue-engineering and regeneration	1560:1625	These findings indicate that Alg/SF hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration.
27156697	1	2	theme	mechanical	149:158	arg1	properties					160:169	adequate mechanical properties	140:169	adequate mechanical properties as well as the ability to supply nutrients and oxygen	140:223	Soft tissue regeneration requires the use of matrices that exhibit adequate mechanical properties as well as the ability to supply nutrients and oxygen, and to remove metabolic bio-products.
27156697	9	3	theme	vein	1322:1325	arg1	HUVECs					1346:1351	HUVECs	1346:1351	HUVECs	1346:1351	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	9	3	theme	vein	1322:1325	arg1	cells					1339:1343	human umbilical vein endothelial cells	1306:1343	human umbilical vein endothelial cells (HUVECs)	1306:1352	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	10	4	theme	Alg/SF	1448:1453	arg1	hydrogels					1455:1463	Alg/SF hydrogels	1448:1463	Alg/SF hydrogels	1448:1463	Viability, attachment, spreading and proliferation of HUVECs were significantly increased on Alg/SF hydrogels compared to neat Alg.
27156697	6	5	theme	thermal	759:765	arg1	behavior					779:786	The thermal degradation behavior	755:786	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF	755:845	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF was dependent on the hydrogel composition and on the geometry of the samples.
27156697	6	5	theme	thermal	759:765	arg1	dependent					851:859	dependent	851:859	dependent	851:859	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF was dependent on the hydrogel composition and on the geometry of the samples.
27156697	6	6	theme	films	791:795	arg1	behavior					779:786	The thermal degradation behavior	755:786	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF	755:845	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF was dependent on the hydrogel composition and on the geometry of the samples.
27156697	6	6	theme	films	791:795	arg1	dependent					851:859	dependent	851:859	dependent	851:859	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF was dependent on the hydrogel composition and on the geometry of the samples.
27156697	6	7	theme	samples	916:922	arg1	geometry					900:907	the geometry	896:907	the geometry of the samples	896:922	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF was dependent on the hydrogel composition and on the geometry of the samples.
27156697	5	8	from	coil	713:716	arg1	SF					698:699	SF	698:699	SF from random coil	698:716	FTIR results indicated a change of conformation of SF from random coil to β-sheet after hydrogel formation.
27156697	5	8	from	coil	713:716	arg1	conformation					682:693	conformation	682:693	conformation of SF from random coil	682:716	FTIR results indicated a change of conformation of SF from random coil to β-sheet after hydrogel formation.
27156697	1	9	theme	metabolic	240:248	arg1	bio-products					250:261	metabolic bio-products	240:261	metabolic bio-products	240:261	Soft tissue regeneration requires the use of matrices that exhibit adequate mechanical properties as well as the ability to supply nutrients and oxygen, and to remove metabolic bio-products.
27156697	3	10	theme	hydrogels	501:509	arg1	2D					518:519	2D	518:519	2D	518:519	Herein, we report two main strategies to combine cells with biomaterials: cells are either seeded onto prefabricated hydrogels films (2D), or encapsulated during hydrogel microcapsules formation (3D).
27156697	3	10	theme	hydrogels	501:509	arg1	films					511:515	prefabricated hydrogels films	487:515	prefabricated hydrogels films (2D)	487:520	Herein, we report two main strategies to combine cells with biomaterials: cells are either seeded onto prefabricated hydrogels films (2D), or encapsulated during hydrogel microcapsules formation (3D).
27156697	11	11	theme	Alg/SF	1516:1521	arg1	material					1547:1554	a promising material	1535:1554	a promising material for the biomedical applications in tissue-engineering and regeneration	1535:1625	These findings indicate that Alg/SF hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration.
27156697	11	11	theme	Alg/SF	1516:1521	arg1	hydrogel					1523:1530	Alg/SF hydrogel	1516:1530	Alg/SF hydrogel	1516:1530	These findings indicate that Alg/SF hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration.
27156697	9	12	theme	different	1262:1270	arg1	geometries					1272:1281	different geometries	1262:1281	different geometries	1262:1281	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	9	13	theme	in	1204:1205	arg1	interaction					1227:1237	the in vitro cell-material interaction	1200:1237	the in vitro cell-material interaction on Alg/SF hydrogels of different geometries	1200:1281	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	8	14	theme	pure	1153:1156	arg1	Alg					1158:1160	pure Alg	1153:1160	pure Alg (6 times higher stiffness)	1153:1187	Mechanical tests showed that addition of SF promotes an increase in storage modulus, leading to a stiffer material as compared with pure Alg (6 times higher stiffness).
27156697	8	14	theme	pure	1153:1156	arg1	stiffness					1178:1186	6 times higher stiffness	1163:1186	6 times higher stiffness	1163:1186	Mechanical tests showed that addition of SF promotes an increase in storage modulus, leading to a stiffer material as compared with pure Alg (6 times higher stiffness).
27156697	8	15	theme	storage	1089:1095	arg1	modulus					1097:1103	storage modulus	1089:1103	storage modulus	1089:1103	Mechanical tests showed that addition of SF promotes an increase in storage modulus, leading to a stiffer material as compared with pure Alg (6 times higher stiffness).
27156697	1	16	theme	Soft	73:76	arg1	regeneration					85:96	Soft tissue regeneration	73:96	Soft tissue regeneration	73:96	Soft tissue regeneration requires the use of matrices that exhibit adequate mechanical properties as well as the ability to supply nutrients and oxygen, and to remove metabolic bio-products.
27156697	11	17	from	applications	1575:1586	arg1	regeneration					1614:1625	regeneration	1614:1625	regeneration	1614:1625	These findings indicate that Alg/SF hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration.
27156697	11	17	from	applications	1575:1586	arg1	tissue-engineering					1591:1608	tissue-engineering	1591:1608	tissue-engineering	1591:1608	These findings indicate that Alg/SF hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration.
27156697	9	18	theme	cell-material	1213:1225	arg1	interaction					1227:1237	the in vitro cell-material interaction	1200:1237	the in vitro cell-material interaction on Alg/SF hydrogels of different geometries	1200:1281	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	7	19	theme	degradation	999:1009	arg1	behavior					1011:1018	the degradation behavior	995:1018	the degradation behavior	995:1018	The presence of SF caused decreased water uptake ability and affected the degradation behavior.
27156697	8	20	theme	SF	1062:1063	arg1	addition					1050:1057	addition	1050:1057	addition of SF	1050:1063	Mechanical tests showed that addition of SF promotes an increase in storage modulus, leading to a stiffer material as compared with pure Alg (6 times higher stiffness).
27156697	1	21	theme	tissue	78:83	arg1	regeneration					85:96	Soft tissue regeneration	73:96	Soft tissue regeneration	73:96	Soft tissue regeneration requires the use of matrices that exhibit adequate mechanical properties as well as the ability to supply nutrients and oxygen, and to remove metabolic bio-products.
27156697	5	22	theme	hydrogel	735:742	arg1	formation					744:752	hydrogel formation	735:752	hydrogel formation	735:752	FTIR results indicated a change of conformation of SF from random coil to β-sheet after hydrogel formation.
27156697	0	23	theme	silk	23:26	arg1	fibroin					28:34	silk fibroin	23:34	silk fibroin	23:34	Soft-matrices based on silk fibroin and alginate for tissue engineering.
27156697	10	24	theme	HUVECs	1409:1414	arg1	attachment					1366:1375	attachment	1366:1375	attachment	1366:1375	Viability, attachment, spreading and proliferation of HUVECs were significantly increased on Alg/SF hydrogels compared to neat Alg.
27156697	10	24	theme	HUVECs	1409:1414	arg1	Viability					1355:1363	Viability	1355:1363	Viability	1355:1363	Viability, attachment, spreading and proliferation of HUVECs were significantly increased on Alg/SF hydrogels compared to neat Alg.
27156697	10	24	theme	HUVECs	1409:1414	arg1	proliferation					1392:1404	proliferation	1392:1404	proliferation	1392:1404	Viability, attachment, spreading and proliferation of HUVECs were significantly increased on Alg/SF hydrogels compared to neat Alg.
27156697	10	24	theme	HUVECs	1409:1414	arg1	spreading					1378:1386	spreading	1378:1386	spreading	1378:1386	Viability, attachment, spreading and proliferation of HUVECs were significantly increased on Alg/SF hydrogels compared to neat Alg.
27156697	9	25	theme	Alg/SF	1242:1247	arg1	hydrogels					1249:1257	Alg/SF hydrogels	1242:1257	Alg/SF hydrogels of different geometries	1242:1281	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	6	26	theme	microcapsules	801:813	arg1	behavior					779:786	The thermal degradation behavior	755:786	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF	755:845	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF was dependent on the hydrogel composition and on the geometry of the samples.
27156697	6	26	theme	microcapsules	801:813	arg1	dependent					851:859	dependent	851:859	dependent	851:859	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF was dependent on the hydrogel composition and on the geometry of the samples.
27156697	3	27	theme	prefabricated	487:499	arg1	2D					518:519	2D	518:519	2D	518:519	Herein, we report two main strategies to combine cells with biomaterials: cells are either seeded onto prefabricated hydrogels films (2D), or encapsulated during hydrogel microcapsules formation (3D).
27156697	3	27	theme	prefabricated	487:499	arg1	films					511:515	prefabricated hydrogels films	487:515	prefabricated hydrogels films (2D)	487:520	Herein, we report two main strategies to combine cells with biomaterials: cells are either seeded onto prefabricated hydrogels films (2D), or encapsulated during hydrogel microcapsules formation (3D).
27156697	5	28	theme	random	706:711	arg1	coil					713:716	random coil	706:716	random coil	706:716	FTIR results indicated a change of conformation of SF from random coil to β-sheet after hydrogel formation.
27156697	5	29	theme	FTIR	647:650	arg1	results					652:658	FTIR results	647:658	FTIR results	647:658	FTIR results indicated a change of conformation of SF from random coil to β-sheet after hydrogel formation.
27156697	1	30	theme	supply	197:202	arg1	nutrients					204:212	supply nutrients	197:212	supply nutrients	197:212	Soft tissue regeneration requires the use of matrices that exhibit adequate mechanical properties as well as the ability to supply nutrients and oxygen, and to remove metabolic bio-products.
27156697	2	31	theme	silk	365:368	arg1	SF					379:380	SF	379:380	SF	379:380	In this work, we describe the development of hydrogels based on the blend between alginate (Alg) and silk fibroin (SF).
27156697	2	31	theme	silk	365:368	arg1	fibroin					370:376	silk fibroin	365:376	silk fibroin (SF)	365:381	In this work, we describe the development of hydrogels based on the blend between alginate (Alg) and silk fibroin (SF).
27156697	8	32	theme	stiffer	1119:1125	arg1	material					1127:1134	a stiffer material	1117:1134	a stiffer material	1117:1134	Mechanical tests showed that addition of SF promotes an increase in storage modulus, leading to a stiffer material as compared with pure Alg (6 times higher stiffness).
27156697	9	33	theme	endothelial	1327:1337	arg1	HUVECs					1346:1351	HUVECs	1346:1351	HUVECs	1346:1351	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	9	33	theme	endothelial	1327:1337	arg1	cells					1339:1343	human umbilical vein endothelial cells	1306:1343	human umbilical vein endothelial cells (HUVECs)	1306:1352	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	3	34	theme	microcapsules	555:567	arg1	3D					580:581	3D	580:581	3D	580:581	Herein, we report two main strategies to combine cells with biomaterials: cells are either seeded onto prefabricated hydrogels films (2D), or encapsulated during hydrogel microcapsules formation (3D).
27156697	3	34	theme	microcapsules	555:567	arg1	formation					569:577	hydrogel microcapsules formation	546:577	hydrogel microcapsules formation (3D)	546:582	Herein, we report two main strategies to combine cells with biomaterials: cells are either seeded onto prefabricated hydrogels films (2D), or encapsulated during hydrogel microcapsules formation (3D).
27156697	8	35	theme	higher	1171:1176	arg1	Alg					1158:1160	pure Alg	1153:1160	pure Alg (6 times higher stiffness)	1153:1187	Mechanical tests showed that addition of SF promotes an increase in storage modulus, leading to a stiffer material as compared with pure Alg (6 times higher stiffness).
27156697	8	35	theme	higher	1171:1176	arg1	stiffness					1178:1186	6 times higher stiffness	1163:1186	6 times higher stiffness	1163:1186	Mechanical tests showed that addition of SF promotes an increase in storage modulus, leading to a stiffer material as compared with pure Alg (6 times higher stiffness).
27156697	6	36	theme	hydrogel	868:875	arg1	composition					877:887	the hydrogel composition	864:887	the hydrogel composition	864:887	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF was dependent on the hydrogel composition and on the geometry of the samples.
27156697	7	37	theme	water	961:965	arg1	ability					974:980	decreased water uptake ability	951:980	decreased water uptake ability	951:980	The presence of SF caused decreased water uptake ability and affected the degradation behavior.
27156697	7	38	theme	SF	941:942	arg1	presence					929:936	The presence	925:936	The presence of SF	925:942	The presence of SF caused decreased water uptake ability and affected the degradation behavior.
27156697	9	39	from	interaction	1227:1237	arg1	hydrogels					1249:1257	Alg/SF hydrogels	1242:1257	Alg/SF hydrogels of different geometries	1242:1281	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	9	40	theme	geometries	1272:1281	arg1	hydrogels					1249:1257	Alg/SF hydrogels	1242:1257	Alg/SF hydrogels of different geometries	1242:1281	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	7	41	theme	uptake	967:972	arg1	ability					974:980	decreased water uptake ability	951:980	decreased water uptake ability	951:980	The presence of SF caused decreased water uptake ability and affected the degradation behavior.
27156697	10	42	theme	neat	1477:1480	arg1	Alg					1482:1484	neat Alg	1477:1484	neat Alg	1477:1484	Viability, attachment, spreading and proliferation of HUVECs were significantly increased on Alg/SF hydrogels compared to neat Alg.
27156697	2	43	theme	hydrogels	309:317	arg1	development					294:304	the development	290:304	the development of hydrogels based on the blend between alginate (Alg) and silk fibroin (SF)	290:381	In this work, we describe the development of hydrogels based on the blend between alginate (Alg) and silk fibroin (SF).
27156697	9	44	dep	in	1204:1205	arg1	vitro					1207:1211	vitro	1207:1211	vitro	1207:1211	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	8	45	theme	Mechanical	1021:1030	arg1	tests					1032:1036	Mechanical tests	1021:1036	Mechanical tests	1021:1036	Mechanical tests showed that addition of SF promotes an increase in storage modulus, leading to a stiffer material as compared with pure Alg (6 times higher stiffness).
27156697	6	46	theme	degradation	767:777	arg1	behavior					779:786	The thermal degradation behavior	755:786	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF	755:845	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF was dependent on the hydrogel composition and on the geometry of the samples.
27156697	6	46	theme	degradation	767:777	arg1	dependent					851:859	dependent	851:859	dependent	851:859	The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF was dependent on the hydrogel composition and on the geometry of the samples.
27156697	1	47	theme	matrices	118:125	arg1	use					111:113	the use	107:113	the use of matrices that exhibit adequate mechanical properties as well as the ability to supply nutrients and oxygen, and to remove metabolic bio-products	107:261	Soft tissue regeneration requires the use of matrices that exhibit adequate mechanical properties as well as the ability to supply nutrients and oxygen, and to remove metabolic bio-products.
27156697	7	48	theme	decreased	951:959	arg1	ability					974:980	decreased water uptake ability	951:980	decreased water uptake ability	951:980	The presence of SF caused decreased water uptake ability and affected the degradation behavior.
27156697	5	49	from	conformation	682:693	arg1	coil					713:716	random coil	706:716	random coil	706:716	FTIR results indicated a change of conformation of SF from random coil to β-sheet after hydrogel formation.
27156697	3	50	theme	hydrogel	546:553	arg1	3D					580:581	3D	580:581	3D	580:581	Herein, we report two main strategies to combine cells with biomaterials: cells are either seeded onto prefabricated hydrogels films (2D), or encapsulated during hydrogel microcapsules formation (3D).
27156697	3	50	theme	hydrogel	546:553	arg1	formation					569:577	hydrogel microcapsules formation	546:577	hydrogel microcapsules formation (3D)	546:582	Herein, we report two main strategies to combine cells with biomaterials: cells are either seeded onto prefabricated hydrogels films (2D), or encapsulated during hydrogel microcapsules formation (3D).
27156697	3	51	theme	main	406:409	arg1	strategies					411:420	two main strategies	402:420	two main strategies to combine cells with biomaterials	402:455	Herein, we report two main strategies to combine cells with biomaterials: cells are either seeded onto prefabricated hydrogels films (2D), or encapsulated during hydrogel microcapsules formation (3D).
27156697	5	52	theme	conformation	682:693	arg1	change					672:677	a change	670:677	a change of conformation of SF from random coil to β-sheet	670:727	FTIR results indicated a change of conformation of SF from random coil to β-sheet after hydrogel formation.
27156697	8	53	from	increase	1077:1084	arg1	modulus					1097:1103	storage modulus	1089:1103	storage modulus	1089:1103	Mechanical tests showed that addition of SF promotes an increase in storage modulus, leading to a stiffer material as compared with pure Alg (6 times higher stiffness).
27156697	11	54	theme	promising	1537:1545	arg1	material					1547:1554	a promising material	1535:1554	a promising material for the biomedical applications in tissue-engineering and regeneration	1535:1625	These findings indicate that Alg/SF hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration.
27156697	11	54	theme	promising	1537:1545	arg1	hydrogel					1523:1530	Alg/SF hydrogel	1516:1530	Alg/SF hydrogel	1516:1530	These findings indicate that Alg/SF hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration.
27156697	9	55	theme	human	1306:1310	arg1	HUVECs					1346:1351	HUVECs	1346:1351	HUVECs	1346:1351	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	9	55	theme	human	1306:1310	arg1	cells					1339:1343	human umbilical vein endothelial cells	1306:1343	human umbilical vein endothelial cells (HUVECs)	1306:1352	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	0	56	theme	tissue	53:58	arg1	engineering					60:70	tissue engineering	53:70	tissue engineering	53:70	Soft-matrices based on silk fibroin and alginate for tissue engineering.
27156697	1	57	theme	adequate	140:147	arg1	properties					160:169	adequate mechanical properties	140:169	adequate mechanical properties as well as the ability to supply nutrients and oxygen	140:223	Soft tissue regeneration requires the use of matrices that exhibit adequate mechanical properties as well as the ability to supply nutrients and oxygen, and to remove metabolic bio-products.
27156697	9	58	theme	umbilical	1312:1320	arg1	HUVECs					1346:1351	HUVECs	1346:1351	HUVECs	1346:1351	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
27156697	9	58	theme	umbilical	1312:1320	arg1	cells					1339:1343	human umbilical vein endothelial cells	1306:1343	human umbilical vein endothelial cells (HUVECs)	1306:1352	Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs).
24564824	3	0	theme	microcapsule	552:563	arg1	graph					539:543	The section graph	527:543	The section graph of APA microcapsule	527:563	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	2	1	theme	membrane	416:423	arg1	thickness					399:407	the thickness	395:407	the thickness of APA membrane	395:423	Specifically, light microscopy with frozen section was used to visualize and quantify the thickness of APA membrane, and monitor temporal changes in the thickness of microcapsules during a month long culture in vitro.
24564824	8	2	theme	other	1695:1699	arg1	types					1701:1705	other types	1695:1705	other types of microcapsules	1695:1722	The paper showed an easy method to prepare APA-Ca and APA-Ba, and examine their thickness and surface, which could be utilized to study other types of microcapsules.
24564824	7	3	theme	vivo	1307:1310	arg1	experiment					1312:1321	vivo experiment	1307:1321	vivo experiment	1307:1321	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	3	4	from	p < 0.05	706:713	arg1	500 ± 100 μm					658:669	500 ± 100 μm	658:669	500 ± 100 μm	658:669	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	4	from	p < 0.05	706:713	arg1	thickness					727:735	layer thickness	721:735	layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05)	721:831	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	4	from	p < 0.05	706:713	arg1	diameter					635:642	diameter 900 ± 100	635:652	diameter 900 ± 100	635:652	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	8	5	theme	microcapsules	1710:1722	arg1	types					1701:1705	other types	1695:1705	other types of microcapsules	1695:1722	The paper showed an easy method to prepare APA-Ca and APA-Ba, and examine their thickness and surface, which could be utilized to study other types of microcapsules.
24564824	6	6	from	results	1126:1132	arg1	surface					1145:1151	the outer surface	1135:1151	the outer surface of APA-Ca and APA-Ba membrane	1135:1181	From the results, the outer surface of APA-Ca and APA-Ba membrane were smooth and dense, the film thickness of the APA-Ca was about 450-690 nm, while the APA-Ba was approximately 335 nm.
24564824	6	6	from	results	1126:1132	arg1	smooth					1188:1193	smooth	1188:1193	smooth	1188:1193	From the results, the outer surface of APA-Ca and APA-Ba membrane were smooth and dense, the film thickness of the APA-Ca was about 450-690 nm, while the APA-Ba was approximately 335 nm.
24564824	3	7	theme	layer	604:608	arg1	thickness					610:618	layer thickness	604:618	layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules	604:906	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	2	8	used	used	364:367	arg2	microscopy					329:338	light microscopy	323:338	light microscopy with frozen section	323:358	Specifically, light microscopy with frozen section was used to visualize and quantify the thickness of APA membrane, and monitor temporal changes in the thickness of microcapsules during a month long culture in vitro.
24564824	5	9	theme	drying	1034:1039	arg1	method					1041:1046	freeze drying method	1027:1046	freeze drying method	1027:1046	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	3	10	theme	thickness	610:618	arg1	measurement					589:599	the accurate measurement	576:599	the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules	576:906	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	11	from	6.01 ± 1.02	674:684	arg1	500 ± 100 μm					658:669	500 ± 100 μm	658:669	500 ± 100 μm	658:669	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	11	from	6.01 ± 1.02	674:684	arg1	thickness					727:735	layer thickness	721:735	layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05)	721:831	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	11	from	6.01 ± 1.02	674:684	arg1	diameter					635:642	diameter 900 ± 100	635:652	diameter 900 ± 100	635:652	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	6	12	theme	APA-Ba	1167:1172	arg1	membrane					1174:1181	APA-Ba membrane	1167:1181	APA-Ba membrane	1167:1181	From the results, the outer surface of APA-Ca and APA-Ba membrane were smooth and dense, the film thickness of the APA-Ca was about 450-690 nm, while the APA-Ba was approximately 335 nm.
24564824	3	13	from	8.21 ± 1.97 μm	807:820	arg1	500 ± 100 μm					658:669	500 ± 100 μm	658:669	500 ± 100 μm	658:669	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	13	from	8.21 ± 1.97 μm	807:820	arg1	thickness					727:735	layer thickness	721:735	layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05)	721:831	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	13	from	8.21 ± 1.97 μm	807:820	arg1	diameter					635:642	diameter 900 ± 100	635:652	diameter 900 ± 100	635:652	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	5	14	dep	surface	1071:1077	arg1	the					1067:1069	the	1067:1069	the	1067:1069	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	6	15	theme	outer	1139:1143	arg1	surface					1145:1151	the outer surface	1135:1151	the outer surface of APA-Ca and APA-Ba membrane	1135:1181	From the results, the outer surface of APA-Ca and APA-Ba membrane were smooth and dense, the film thickness of the APA-Ca was about 450-690 nm, while the APA-Ba was approximately 335 nm.
24564824	6	15	theme	outer	1139:1143	arg1	smooth					1188:1193	smooth	1188:1193	smooth	1188:1193	From the results, the outer surface of APA-Ca and APA-Ba membrane were smooth and dense, the film thickness of the APA-Ca was about 450-690 nm, while the APA-Ba was approximately 335 nm.
24564824	6	16	theme	membrane	1174:1181	arg1	surface					1145:1151	the outer surface	1135:1151	the outer surface of APA-Ca and APA-Ba membrane	1135:1181	From the results, the outer surface of APA-Ca and APA-Ba membrane were smooth and dense, the film thickness of the APA-Ca was about 450-690 nm, while the APA-Ba was approximately 335 nm.
24564824	6	16	theme	membrane	1174:1181	arg1	smooth					1188:1193	smooth	1188:1193	smooth	1188:1193	From the results, the outer surface of APA-Ca and APA-Ba membrane were smooth and dense, the film thickness of the APA-Ca was about 450-690 nm, while the APA-Ba was approximately 335 nm.
24564824	0	17	theme	Preparation	0:10	arg1	examination					27:37	Preparation and microscopy examination	0:37	Preparation and microscopy examination of alginate-poly-L-lysine-alginate	0:72	Preparation and microscopy examination of alginate-poly-L-lysine-alginate microcapsules.
24564824	7	18	from	thickness	1385:1393	arg1	site					1431:1434	intrapertioneal site	1415:1434	intrapertioneal site for 30 days after transplantation (p > 0.05)	1415:1479	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	8	19	theme	easy	1579:1582	arg1	method					1584:1589	an easy method	1576:1589	an easy method	1576:1589	The paper showed an easy method to prepare APA-Ca and APA-Ba, and examine their thickness and surface, which could be utilized to study other types of microcapsules.
24564824	8	20	dep	method	1584:1589	arg1	prepare					1594:1600	prepare	1594:1600	prepare APA-Ca and APA-Ba	1594:1618	The paper showed an easy method to prepare APA-Ca and APA-Ba, and examine their thickness and surface, which could be utilized to study other types of microcapsules.
24564824	8	20	dep	method	1584:1589	arg1	examine					1625:1631	examine	1625:1631	examine their thickness and surface	1625:1659	The paper showed an easy method to prepare APA-Ca and APA-Ba, and examine their thickness and surface, which could be utilized to study other types of microcapsules.
24564824	5	21	theme	electron	1002:1009	arg1	microscopy					1011:1020	Field emission scanning electron microscopy	978:1020	Field emission scanning electron microscopy with freeze drying method	978:1046	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	3	22	with	APA-Ba	740:745	arg1	diameter					752:759	diameter 900 ± 100 and 500 ± 100 μm	752:786	diameter	752:759	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	22	with	APA-Ba	740:745	arg1	500 ± 100 μm					775:786	500 ± 100 μm	775:786	500 ± 100 μm	775:786	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	2	23	theme	long	504:507	arg1	culture					509:515	a month long culture	496:515	a month long culture	496:515	Specifically, light microscopy with frozen section was used to visualize and quantify the thickness of APA membrane, and monitor temporal changes in the thickness of microcapsules during a month long culture in vitro.
24564824	3	24	theme	APA-Ca	623:628	arg1	thickness					610:618	layer thickness	604:618	layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules	604:906	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	8	25	used	utilized	1677:1684	arg2	method					1584:1589	an easy method	1576:1589	an easy method	1576:1589	The paper showed an easy method to prepare APA-Ca and APA-Ba, and examine their thickness and surface, which could be utilized to study other types of microcapsules.
24564824	1	26	theme	Ba-alginate-poly-l-lysine-alginate	137:170	arg1	microcapsules					181:193	Ca-alginate-poly-l-lysine-alginate (APA-Ca) and Ba-alginate-poly-l-lysine-alginate (APA-Ba) microcapsules	89:193	microcapsules	181:193	Ca-alginate-poly-l-lysine-alginate (APA-Ca) and Ba-alginate-poly-l-lysine-alginate (APA-Ba) microcapsules were prepared and their thickness and surface were examined by light microscopy and scanning electron microscopy.
24564824	7	27	theme	significant	1331:1341	arg1	difference					1343:1352	little significant difference	1324:1352	little significant difference	1324:1352	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	2	28	theme	temporal	438:445	arg1	changes					447:453	temporal changes	438:453	temporal changes in the thickness of microcapsules	438:487	Specifically, light microscopy with frozen section was used to visualize and quantify the thickness of APA membrane, and monitor temporal changes in the thickness of microcapsules during a month long culture in vitro.
24564824	2	29	theme	month	498:502	arg1	culture					509:515	a month long culture	496:515	a month long culture	496:515	Specifically, light microscopy with frozen section was used to visualize and quantify the thickness of APA membrane, and monitor temporal changes in the thickness of microcapsules during a month long culture in vitro.
24564824	7	30	theme	film	1380:1383	arg1	thickness					1385:1393	film thickness	1380:1393	film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules	1380:1556	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	7	31	from	microcapsules	1398:1410	arg1	site					1431:1434	intrapertioneal site	1415:1434	intrapertioneal site for 30 days after transplantation (p > 0.05)	1415:1479	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	1	32	theme	light	258:262	arg1	microscopy					264:273	light microscopy	258:273	light microscopy	258:273	Ca-alginate-poly-l-lysine-alginate (APA-Ca) and Ba-alginate-poly-l-lysine-alginate (APA-Ba) microcapsules were prepared and their thickness and surface were examined by light microscopy and scanning electron microscopy.
24564824	0	33	theme	microscopy	16:25	arg1	examination					27:37	Preparation and microscopy examination	0:37	Preparation and microscopy examination of alginate-poly-L-lysine-alginate	0:72	Preparation and microscopy examination of alginate-poly-L-lysine-alginate microcapsules.
24564824	1	34	theme	APA-Ba	173:178	arg1	microcapsules					181:193	Ca-alginate-poly-l-lysine-alginate (APA-Ca) and Ba-alginate-poly-l-lysine-alginate (APA-Ba) microcapsules	89:193	microcapsules	181:193	Ca-alginate-poly-l-lysine-alginate (APA-Ca) and Ba-alginate-poly-l-lysine-alginate (APA-Ba) microcapsules were prepared and their thickness and surface were examined by light microscopy and scanning electron microscopy.
24564824	2	35	theme	microcapsules	475:487	arg1	thickness					462:470	the thickness	458:470	the thickness of microcapsules	458:487	Specifically, light microscopy with frozen section was used to visualize and quantify the thickness of APA membrane, and monitor temporal changes in the thickness of microcapsules during a month long culture in vitro.
24564824	0	36	theme	alginate-poly-L-lysine-alginate	42:72	arg1	examination					27:37	Preparation and microscopy examination	0:37	Preparation and microscopy examination of alginate-poly-L-lysine-alginate	0:72	Preparation and microscopy examination of alginate-poly-L-lysine-alginate microcapsules.
24564824	5	37	theme	Field	978:982	arg1	microscopy					1011:1020	Field emission scanning electron microscopy	978:1020	Field emission scanning electron microscopy with freeze drying method	978:1046	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	3	38	with	APA-Ca	623:628	arg1	500 ± 100 μm					658:669	500 ± 100 μm	658:669	500 ± 100 μm	658:669	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	38	with	APA-Ca	623:628	arg1	thickness					727:735	layer thickness	721:735	layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05)	721:831	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	38	with	APA-Ca	623:628	arg1	diameter					635:642	diameter 900 ± 100	635:652	diameter 900 ± 100	635:652	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	7	39	theme	intrapertioneal	1415:1429	arg1	site					1431:1434	intrapertioneal site	1415:1434	intrapertioneal site for 30 days after transplantation (p > 0.05)	1415:1479	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	6	40	theme	APA-Ca	1232:1237	arg1	thickness					1215:1223	the film thickness	1206:1223	the film thickness of the APA-Ca	1206:1237	From the results, the outer surface of APA-Ca and APA-Ba membrane were smooth and dense, the film thickness of the APA-Ca was about 450-690 nm, while the APA-Ba was approximately 335 nm.
24564824	7	41	theme	little	1324:1329	arg1	difference					1343:1352	little significant difference	1324:1352	little significant difference	1324:1352	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	3	42	from	5.47 ± 0.90	791:801	arg1	500 ± 100 μm					658:669	500 ± 100 μm	658:669	500 ± 100 μm	658:669	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	42	from	5.47 ± 0.90	791:801	arg1	thickness					727:735	layer thickness	721:735	layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05)	721:831	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	42	from	5.47 ± 0.90	791:801	arg1	diameter					635:642	diameter 900 ± 100	635:652	diameter 900 ± 100	635:652	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	2	43	from	changes	447:453	arg1	thickness					462:470	the thickness	458:470	the thickness of microcapsules	458:487	Specifically, light microscopy with frozen section was used to visualize and quantify the thickness of APA membrane, and monitor temporal changes in the thickness of microcapsules during a month long culture in vitro.
24564824	1	44	theme	scanning	279:286	arg1	microscopy					297:306	scanning electron microscopy	279:306	scanning electron microscopy	279:306	Ca-alginate-poly-l-lysine-alginate (APA-Ca) and Ba-alginate-poly-l-lysine-alginate (APA-Ba) microcapsules were prepared and their thickness and surface were examined by light microscopy and scanning electron microscopy.
24564824	7	45	theme	microcapsules	1398:1410	arg1	thickness					1385:1393	film thickness	1380:1393	film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules	1380:1556	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	2	46	theme	APA	412:414	arg1	membrane					416:423	APA membrane	412:423	APA membrane	412:423	Specifically, light microscopy with frozen section was used to visualize and quantify the thickness of APA membrane, and monitor temporal changes in the thickness of microcapsules during a month long culture in vitro.
24564824	5	47	theme	microcapsules	1102:1114	arg1	thickness					1083:1091	thickness	1083:1091	thickness	1083:1091	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	5	47	theme	microcapsules	1102:1114	arg1	surface					1071:1077	surface	1071:1077	surface	1071:1077	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	5	48	theme	dried	1096:1100	arg1	microcapsules					1102:1114	dried microcapsules	1096:1114	dried microcapsules	1096:1114	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	3	49	theme	APA	548:550	arg1	microcapsule					552:563	APA microcapsule	548:563	APA microcapsule	548:563	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	2	50	with	microscopy	329:338	arg1	section					352:358	frozen section	345:358	frozen section	345:358	Specifically, light microscopy with frozen section was used to visualize and quantify the thickness of APA membrane, and monitor temporal changes in the thickness of microcapsules during a month long culture in vitro.
24564824	3	51	theme	APA-Ba	740:745	arg1	500 ± 100 μm					658:669	500 ± 100 μm	658:669	500 ± 100 μm	658:669	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	51	theme	APA-Ba	740:745	arg1	thickness					727:735	layer thickness	721:735	layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05)	721:831	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	3	51	theme	APA-Ba	740:745	arg1	diameter					635:642	diameter 900 ± 100	635:652	diameter 900 ± 100	635:652	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	7	52	theme	thickness	1385:1393	arg1	change					1370:1375	the change	1366:1375	the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules	1366:1556	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	3	53	theme	section	531:537	arg1	graph					539:543	The section graph	527:543	The section graph of APA microcapsule	527:563	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	7	54	from	site	1431:1434	arg1	thickness					1385:1393	film thickness	1380:1393	film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules	1380:1556	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	1	55	theme	electron	288:295	arg1	microscopy					297:306	scanning electron microscopy	279:306	scanning electron microscopy	279:306	Ca-alginate-poly-l-lysine-alginate (APA-Ca) and Ba-alginate-poly-l-lysine-alginate (APA-Ba) microcapsules were prepared and their thickness and surface were examined by light microscopy and scanning electron microscopy.
24564824	6	56	theme	film	1210:1213	arg1	thickness					1215:1223	the film thickness	1206:1223	the film thickness of the APA-Ca	1206:1237	From the results, the outer surface of APA-Ca and APA-Ba membrane were smooth and dense, the film thickness of the APA-Ca was about 450-690 nm, while the APA-Ba was approximately 335 nm.
24564824	3	57	theme	layer	721:725	arg1	thickness					727:735	layer thickness	721:735	layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05)	721:831	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	1	58	theme	Ca-alginate-poly-l-lysine-alginate	89:122	arg1	APA-Ca					125:130	Ca-alginate-poly-l-lysine-alginate (APA-Ca) and Ba-alginate-poly-l-lysine-alginate (APA-Ba) microcapsules	89:193	APA-Ca	125:130	Ca-alginate-poly-l-lysine-alginate (APA-Ca) and Ba-alginate-poly-l-lysine-alginate (APA-Ba) microcapsules were prepared and their thickness and surface were examined by light microscopy and scanning electron microscopy.
24564824	5	59	theme	scanning	993:1000	arg1	microscopy					1011:1020	Field emission scanning electron microscopy	978:1020	Field emission scanning electron microscopy with freeze drying method	978:1046	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	5	60	theme	emission	984:991	arg1	microscopy					1011:1020	Field emission scanning electron microscopy	978:1020	Field emission scanning electron microscopy with freeze drying method	978:1046	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	6	61	theme	APA-Ca	1156:1161	arg1	surface					1145:1151	the outer surface	1135:1151	the outer surface of APA-Ca and APA-Ba membrane	1135:1181	From the results, the outer surface of APA-Ca and APA-Ba membrane were smooth and dense, the film thickness of the APA-Ca was about 450-690 nm, while the APA-Ba was approximately 335 nm.
24564824	6	61	theme	APA-Ca	1156:1161	arg1	smooth					1188:1193	smooth	1188:1193	smooth	1188:1193	From the results, the outer surface of APA-Ca and APA-Ba membrane were smooth and dense, the film thickness of the APA-Ca was about 450-690 nm, while the APA-Ba was approximately 335 nm.
24564824	2	62	theme	light	323:327	arg1	microscopy					329:338	light microscopy	323:338	light microscopy with frozen section	323:358	Specifically, light microscopy with frozen section was used to visualize and quantify the thickness of APA membrane, and monitor temporal changes in the thickness of microcapsules during a month long culture in vitro.
24564824	5	63	used	used	1052:1055	arg2	microscopy					1011:1020	Field emission scanning electron microscopy	978:1020	Field emission scanning electron microscopy with freeze drying method	978:1046	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	5	64	theme	freeze	1027:1032	arg1	method					1041:1046	freeze drying method	1027:1046	freeze drying method	1027:1046	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	3	65	theme	alginate	852:859	arg1	composition					861:871	the alginate composition	848:871	the alginate composition	848:871	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
24564824	7	66	theme	APA-Ba	1510:1515	arg1	higher					1521:1526	higher	1521:1526	higher	1521:1526	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	7	66	theme	APA-Ba	1510:1515	arg1	recovery					1498:1505	the recovery	1494:1505	the recovery of APA-Ba	1494:1515	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	5	67	with	microscopy	1011:1020	arg1	method					1041:1046	freeze drying method	1027:1046	freeze drying method	1027:1046	Field emission scanning electron microscopy with freeze drying method was used to detect the surface and thickness of dried microcapsules.
24564824	7	68	theme	APA-Ca	1537:1542	arg1	microcapsules					1544:1556	the APA-Ca microcapsules	1533:1556	the APA-Ca microcapsules	1533:1556	In vivo experiment, little significant difference was seen in the change of film thickness of microcapsules in intrapertioneal site for 30 days after transplantation (p > 0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules.
24564824	3	69	theme	accurate	580:587	arg1	measurement					589:599	the accurate measurement	576:599	the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules	576:906	The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900 ± 100 and 500 ± 100 μm at 6.01 ± 1.02 and 9.54 ± 2.42 μm (p < 0.05), and layer thickness of APA-Ba with diameter 900 ± 100 and 500 ± 100 μm at 5.47 ± 0.90 and 8.21 ± 1.97 μm (p < 0.05), regardless of the alginate composition used to generate the microcapsules.
28528949	4	0	used	used	477:480	arg2	agent					488:492	an agent	485:492	an agent in preparation of silver nanoparticles	485:531	In this study, the microbial bioflocculant polymer was used as an agent in preparation of silver nanoparticles.
28528949	4	0	used	used	477:480	arg2	polymer					465:471	the microbial bioflocculant polymer	437:471	the microbial bioflocculant polymer	437:471	In this study, the microbial bioflocculant polymer was used as an agent in preparation of silver nanoparticles.
28528949	4	1	theme	nanoparticles	519:531	arg1	preparation					497:507	preparation	497:507	preparation of silver nanoparticles	497:531	In this study, the microbial bioflocculant polymer was used as an agent in preparation of silver nanoparticles.
28528949	6	2	dep	Microscopic	705:715	arg1	SEM					718:720	SEM	718:720	SEM	718:720	The presence of silver nanoparticles inside the cellulose matrix was confirmed by Scanning Electron Microscopic (SEM) and Transmission Electron Microscopic (TEM) analyses.
28528949	6	2	dep	Microscopic	705:715	arg1	TEM					762:764	TEM	762:764	TEM	762:764	The presence of silver nanoparticles inside the cellulose matrix was confirmed by Scanning Electron Microscopic (SEM) and Transmission Electron Microscopic (TEM) analyses.
28528949	3	3	theme	different	351:359	arg1	agents					372:377	different biological agents	351:377	different biological agents	351:377	In nano-biotechnology people are using different biological agents in the preparation of metal nanoparticles.
28528949	10	4	theme	E.Coli	1158:1163	arg1	bacteria					1165:1172	E.Coli bacteria	1158:1172	E.Coli bacteria	1158:1172	The antibacterial activity of silver nanocomposites was tested against E.Coli bacteria.
28528949	7	5	from	presence	824:831	arg1	nanocomposites					860:873	the cellulose nanocomposites	846:873	the cellulose nanocomposites	846:873	The FTIR characterization studies revealed the presence of silver in the cellulose nanocomposites.
28528949	7	6	theme	silver	836:841	arg1	presence					824:831	the presence	820:831	the presence of silver in the cellulose nanocomposites	820:873	The FTIR characterization studies revealed the presence of silver in the cellulose nanocomposites.
28528949	1	7	theme	repeated	179:186	arg1	units					188:192	repeated units	179:192	repeated units of macromolecules	179:210	Microbial secreted polymers have high molecular weight due to the presence of repeated units of macromolecules.
28528949	8	8	theme	peak	914:917	arg1	formation					919:927	the silver peak formation	903:927	the silver peak formation inside the silver nanocomposites	903:960	The XRD analysis indicated the silver peak formation inside the silver nanocomposites.
28528949	9	9	theme	nanocomposites	1005:1018	arg1	studies					987:993	The thermal degradation studies	963:993	The thermal degradation studies of silver nanocomposites	963:1018	The thermal degradation studies of silver nanocomposites showed that at 450°C the residual weight was completely decreased.
28528949	8	10	theme	silver	907:912	arg1	formation					919:927	the silver peak formation	903:927	the silver peak formation inside the silver nanocomposites	903:960	The XRD analysis indicated the silver peak formation inside the silver nanocomposites.
28528949	2	11	attach	derived	238:244	arg2	polymers					225:232	the polymers	221:232	the polymers	221:232	Some of the polymers are derived from natural resources, including marine and soil microorganisms.
28528949	2	11	attach	derived	238:244	arg1	soil					291:294	soil	291:294	soil	291:294	Some of the polymers are derived from natural resources, including marine and soil microorganisms.
28528949	2	11	attach	derived	238:244	arg2	Some					213:216	Some	213:216	Some	213:216	Some of the polymers are derived from natural resources, including marine and soil microorganisms.
28528949	2	11	attach	derived	238:244	arg1	resources					259:267	natural resources	251:267	natural resources	251:267	Some of the polymers are derived from natural resources, including marine and soil microorganisms.
28528949	2	11	attach	derived	238:244	arg1	marine					280:285	marine	280:285	marine	280:285	Some of the polymers are derived from natural resources, including marine and soil microorganisms.
28528949	7	12	attach	presence	824:831	arg2	silver					836:841	silver	836:841	silver	836:841	The FTIR characterization studies revealed the presence of silver in the cellulose nanocomposites.
28528949	7	12	attach	presence	824:831	arg1	nanocomposites					860:873	the cellulose nanocomposites	846:873	the cellulose nanocomposites	846:873	The FTIR characterization studies revealed the presence of silver in the cellulose nanocomposites.
28528949	1	13	theme	macromolecules	197:210	arg1	units					188:192	repeated units	179:192	repeated units of macromolecules	179:210	Microbial secreted polymers have high molecular weight due to the presence of repeated units of macromolecules.
28528949	1	14	theme	units	188:192	arg1	presence					167:174	the presence	163:174	the presence of repeated units of macromolecules	163:210	Microbial secreted polymers have high molecular weight due to the presence of repeated units of macromolecules.
28528949	4	15	theme	silver	512:517	arg1	nanoparticles					519:531	silver nanoparticles	512:531	silver nanoparticles	512:531	In this study, the microbial bioflocculant polymer was used as an agent in preparation of silver nanoparticles.
28528949	1	16	theme	Microbial	101:109	arg1	polymers					120:127	Microbial secreted polymers	101:127	Microbial secreted polymers	101:127	Microbial secreted polymers have high molecular weight due to the presence of repeated units of macromolecules.
28528949	6	17	theme	Microscopic	749:759	arg1	analyses					767:774	Scanning Electron Microscopic (SEM) and Transmission Electron Microscopic (TEM) analyses	687:774	Scanning Electron Microscopic (SEM) and Transmission Electron Microscopic (TEM) analyses	687:774	The presence of silver nanoparticles inside the cellulose matrix was confirmed by Scanning Electron Microscopic (SEM) and Transmission Electron Microscopic (TEM) analyses.
28528949	6	18	theme	cellulose	653:661	arg1	matrix					663:668	the cellulose matrix	649:668	the cellulose matrix	649:668	The presence of silver nanoparticles inside the cellulose matrix was confirmed by Scanning Electron Microscopic (SEM) and Transmission Electron Microscopic (TEM) analyses.
28528949	9	19	theme	silver	998:1003	arg1	nanocomposites					1005:1018	silver nanocomposites	998:1018	silver nanocomposites	998:1018	The thermal degradation studies of silver nanocomposites showed that at 450°C the residual weight was completely decreased.
28528949	9	20	theme	degradation	975:985	arg1	studies					987:993	The thermal degradation studies	963:993	The thermal degradation studies of silver nanocomposites	963:1018	The thermal degradation studies of silver nanocomposites showed that at 450°C the residual weight was completely decreased.
28528949	1	21	theme	secreted	111:118	arg1	polymers					120:127	Microbial secreted polymers	101:127	Microbial secreted polymers	101:127	Microbial secreted polymers have high molecular weight due to the presence of repeated units of macromolecules.
28528949	5	22	theme	prepared	538:545	arg1	nanoparticles					547:559	The prepared nanoparticles	534:559	The prepared nanoparticles	534:559	The prepared nanoparticles were immobilized into the cellulose matrix.
28528949	2	23	dep	marine	280:285	arg1	microorganisms					296:309	microorganisms	296:309	microorganisms	296:309	Some of the polymers are derived from natural resources, including marine and soil microorganisms.
28528949	7	24	theme	cellulose	850:858	arg1	nanocomposites					860:873	the cellulose nanocomposites	846:873	the cellulose nanocomposites	846:873	The FTIR characterization studies revealed the presence of silver in the cellulose nanocomposites.
28528949	3	25	theme	metal	401:405	arg1	nanoparticles					407:419	metal nanoparticles	401:419	metal nanoparticles	401:419	In nano-biotechnology people are using different biological agents in the preparation of metal nanoparticles.
28528949	4	26	from	agent	488:492	arg1	preparation					497:507	preparation	497:507	preparation of silver nanoparticles	497:531	In this study, the microbial bioflocculant polymer was used as an agent in preparation of silver nanoparticles.
28528949	3	27	theme	nanoparticles	407:419	arg1	preparation					386:396	the preparation	382:396	the preparation of metal nanoparticles	382:419	In nano-biotechnology people are using different biological agents in the preparation of metal nanoparticles.
28528949	1	28	theme	high	134:137	arg1	weight					149:154	high molecular weight	134:154	high molecular weight due to the presence of repeated units of macromolecules	134:210	Microbial secreted polymers have high molecular weight due to the presence of repeated units of macromolecules.
28528949	6	29	theme	silver	621:626	arg1	nanoparticles					628:640	silver nanoparticles	621:640	silver nanoparticles	621:640	The presence of silver nanoparticles inside the cellulose matrix was confirmed by Scanning Electron Microscopic (SEM) and Transmission Electron Microscopic (TEM) analyses.
28528949	2	30	theme	natural	251:257	arg1	soil					291:294	soil	291:294	soil	291:294	Some of the polymers are derived from natural resources, including marine and soil microorganisms.
28528949	2	30	theme	natural	251:257	arg1	resources					259:267	natural resources	251:267	natural resources	251:267	Some of the polymers are derived from natural resources, including marine and soil microorganisms.
28528949	2	30	theme	natural	251:257	arg1	marine					280:285	marine	280:285	marine	280:285	Some of the polymers are derived from natural resources, including marine and soil microorganisms.
28528949	0	31	theme	cellulose/silver	34:49	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of cellulose/silver nanocomposites from bioflocculant reducing agent.
28528949	0	31	theme	cellulose/silver	34:49	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of cellulose/silver nanocomposites from bioflocculant reducing agent.
28528949	10	32	theme	antibacterial	1091:1103	arg1	activity					1105:1112	The antibacterial activity	1087:1112	The antibacterial activity of silver nanocomposites	1087:1137	The antibacterial activity of silver nanocomposites was tested against E.Coli bacteria.
28528949	9	33	theme	residual	1045:1052	arg1	weight					1054:1059	the residual weight	1041:1059	the residual weight	1041:1059	The thermal degradation studies of silver nanocomposites showed that at 450°C the residual weight was completely decreased.
28528949	0	34	theme	bioflocculant	71:83	arg1	agent					94:98	bioflocculant reducing agent	71:98	bioflocculant reducing agent	71:98	Synthesis and characterization of cellulose/silver nanocomposites from bioflocculant reducing agent.
28528949	1	35	contain	have	129:132	arg1	polymers					120:127	Microbial secreted polymers	101:127	Microbial secreted polymers	101:127	Microbial secreted polymers have high molecular weight due to the presence of repeated units of macromolecules.
28528949	1	35	contain	have	129:132	arg2	weight					149:154	high molecular weight	134:154	high molecular weight due to the presence of repeated units of macromolecules	134:210	Microbial secreted polymers have high molecular weight due to the presence of repeated units of macromolecules.
28528949	5	36	theme	cellulose	587:595	arg1	matrix					597:602	the cellulose matrix	583:602	the cellulose matrix	583:602	The prepared nanoparticles were immobilized into the cellulose matrix.
28528949	1	37	theme	molecular	139:147	arg1	weight					149:154	high molecular weight	134:154	high molecular weight due to the presence of repeated units of macromolecules	134:210	Microbial secreted polymers have high molecular weight due to the presence of repeated units of macromolecules.
28528949	4	38	theme	bioflocculant	451:463	arg1	polymer					465:471	the microbial bioflocculant polymer	437:471	the microbial bioflocculant polymer	437:471	In this study, the microbial bioflocculant polymer was used as an agent in preparation of silver nanoparticles.
28528949	4	38	theme	bioflocculant	451:463	arg1	agent					488:492	an agent	485:492	an agent in preparation of silver nanoparticles	485:531	In this study, the microbial bioflocculant polymer was used as an agent in preparation of silver nanoparticles.
28528949	4	39	theme	microbial	441:449	arg1	polymer					465:471	the microbial bioflocculant polymer	437:471	the microbial bioflocculant polymer	437:471	In this study, the microbial bioflocculant polymer was used as an agent in preparation of silver nanoparticles.
28528949	4	39	theme	microbial	441:449	arg1	agent					488:492	an agent	485:492	an agent in preparation of silver nanoparticles	485:531	In this study, the microbial bioflocculant polymer was used as an agent in preparation of silver nanoparticles.
28528949	6	40	theme	Microscopic	705:715	arg1	analyses					767:774	Scanning Electron Microscopic (SEM) and Transmission Electron Microscopic (TEM) analyses	687:774	Scanning Electron Microscopic (SEM) and Transmission Electron Microscopic (TEM) analyses	687:774	The presence of silver nanoparticles inside the cellulose matrix was confirmed by Scanning Electron Microscopic (SEM) and Transmission Electron Microscopic (TEM) analyses.
28528949	3	41	theme	nano-biotechnology	315:332	arg1	people					334:339	nano-biotechnology people	315:339	nano-biotechnology people	315:339	In nano-biotechnology people are using different biological agents in the preparation of metal nanoparticles.
28528949	10	42	theme	nanocomposites	1124:1137	arg1	activity					1105:1112	The antibacterial activity	1087:1112	The antibacterial activity of silver nanocomposites	1087:1137	The antibacterial activity of silver nanocomposites was tested against E.Coli bacteria.
28528949	9	43	theme	thermal	967:973	arg1	studies					987:993	The thermal degradation studies	963:993	The thermal degradation studies of silver nanocomposites	963:1018	The thermal degradation studies of silver nanocomposites showed that at 450°C the residual weight was completely decreased.
28528949	8	44	theme	silver	940:945	arg1	nanocomposites					947:960	the silver nanocomposites	936:960	the silver nanocomposites	936:960	The XRD analysis indicated the silver peak formation inside the silver nanocomposites.
28528949	3	45	theme	biological	361:370	arg1	agents					372:377	different biological agents	351:377	different biological agents	351:377	In nano-biotechnology people are using different biological agents in the preparation of metal nanoparticles.
28528949	10	46	theme	silver	1117:1122	arg1	nanocomposites					1124:1137	silver nanocomposites	1117:1137	silver nanocomposites	1117:1137	The antibacterial activity of silver nanocomposites was tested against E.Coli bacteria.
28528949	8	47	theme	XRD	880:882	arg1	analysis					884:891	The XRD analysis	876:891	The XRD analysis	876:891	The XRD analysis indicated the silver peak formation inside the silver nanocomposites.
28528949	7	48	theme	FTIR	781:784	arg1	studies					803:809	The FTIR characterization studies	777:809	The FTIR characterization studies	777:809	The FTIR characterization studies revealed the presence of silver in the cellulose nanocomposites.
28528949	0	49	theme	reducing	85:92	arg1	agent					94:98	bioflocculant reducing agent	71:98	bioflocculant reducing agent	71:98	Synthesis and characterization of cellulose/silver nanocomposites from bioflocculant reducing agent.
28528949	6	50	theme	nanoparticles	628:640	arg1	presence					609:616	The presence	605:616	The presence of silver nanoparticles inside the cellulose matrix	605:668	The presence of silver nanoparticles inside the cellulose matrix was confirmed by Scanning Electron Microscopic (SEM) and Transmission Electron Microscopic (TEM) analyses.
28528949	7	51	theme	characterization	786:801	arg1	studies					803:809	The FTIR characterization studies	777:809	The FTIR characterization studies	777:809	The FTIR characterization studies revealed the presence of silver in the cellulose nanocomposites.
26794738	0	0	theme	laurate-modified	94:109	arg1	nanoparticles					116:128	sodium laurate-modified TiO2 nanoparticles	87:128	sodium laurate-modified TiO2 nanoparticles	87:128	Enhanced physicochemical properties of chitosan/whey protein isolate composite film by sodium laurate-modified TiO2 nanoparticles.
26794738	5	1	dep	strength	792:799	arg1	the					780:782	the	780:782	the	780:782	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	7	2	theme	materials	1201:1209	arg1	development					1165:1175	the development	1161:1175	the development of novel food packaging materials	1161:1209	The findings contributed to the development of novel food packaging materials.
26794738	1	3	theme	laurate-modified	191:206	arg1	nanoparticles					213:225	sodium laurate-modified TiO2 nanoparticles	184:225	sodium laurate-modified TiO2 nanoparticles	184:225	Chitosan/whey protein isolate film incorporated with sodium laurate-modified TiO2 nanoparticles was developed.
26794738	0	4	theme	sodium	87:92	arg1	nanoparticles					116:128	sodium laurate-modified TiO2 nanoparticles	87:128	sodium laurate-modified TiO2 nanoparticles	87:128	Enhanced physicochemical properties of chitosan/whey protein isolate composite film by sodium laurate-modified TiO2 nanoparticles.
26794738	6	5	theme	nanocomposite	979:991	arg1	film					993:996	nanocomposite film	979:996	nanocomposite film	979:996	The equilibrium moisture of nanocomposite film was lower than binary film, and its water sorption isotherm of the nanocomposite film fitted well to Guggenheim-Anderson-deBoer model.
26794738	1	6	theme	TiO2	208:211	arg1	nanoparticles					213:225	sodium laurate-modified TiO2 nanoparticles	184:225	sodium laurate-modified TiO2 nanoparticles	184:225	Chitosan/whey protein isolate film incorporated with sodium laurate-modified TiO2 nanoparticles was developed.
26794738	7	7	theme	food	1186:1189	arg1	materials					1201:1209	novel food packaging materials	1180:1209	novel food packaging materials	1180:1209	The findings contributed to the development of novel food packaging materials.
26794738	3	8	theme	chitosan	622:629	arg1	compatibility					580:592	the compatibility	576:592	the compatibility of whey protein isolate and chitosan	576:629	Our results showed that the nanoparticles improved the compatibility of whey protein isolate and chitosan.
26794738	3	9	dep	protein	602:608	arg1	isolate					610:616	isolate	610:616	isolate	610:616	Our results showed that the nanoparticles improved the compatibility of whey protein isolate and chitosan.
26794738	4	10	theme	chitosan/whey	685:697	arg1	film					715:718	chitosan/whey protein isolate film	685:718	chitosan/whey protein isolate film	685:718	Addition of nanoparticles increased the whiteness of chitosan/whey protein isolate film, but decreased its transparency.
26794738	0	11	theme	TiO2	111:114	arg1	nanoparticles					116:128	sodium laurate-modified TiO2 nanoparticles	87:128	sodium laurate-modified TiO2 nanoparticles	87:128	Enhanced physicochemical properties of chitosan/whey protein isolate composite film by sodium laurate-modified TiO2 nanoparticles.
26794738	2	12	theme	scanning	351:358	arg1	calorimetry					360:370	differential scanning calorimetry	338:370	differential scanning calorimetry	338:370	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	2	13	theme	tensile	430:436	arg1	strength					438:445	tensile strength	430:445	tensile strength	430:445	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	6	14	theme	sorption	1040:1047	arg1	isotherm					1049:1056	its water sorption isotherm	1030:1056	its water sorption isotherm of the nanocomposite film	1030:1082	The equilibrium moisture of nanocomposite film was lower than binary film, and its water sorption isotherm of the nanocomposite film fitted well to Guggenheim-Anderson-deBoer model.
26794738	5	15	theme	nanocomposite	828:840	arg1	film					842:845	nanocomposite film	828:845	nanocomposite film	828:845	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	6	16	theme	equilibrium	955:965	arg1	lower					1002:1006	lower	1002:1006	lower	1002:1006	The equilibrium moisture of nanocomposite film was lower than binary film, and its water sorption isotherm of the nanocomposite film fitted well to Guggenheim-Anderson-deBoer model.
26794738	6	16	theme	equilibrium	955:965	arg1	moisture					967:974	The equilibrium moisture	951:974	The equilibrium moisture of nanocomposite film	951:996	The equilibrium moisture of nanocomposite film was lower than binary film, and its water sorption isotherm of the nanocomposite film fitted well to Guggenheim-Anderson-deBoer model.
26794738	2	17	theme	differential	338:349	arg1	calorimetry					360:370	differential scanning calorimetry	338:370	differential scanning calorimetry	338:370	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	5	18	theme	film	842:845	arg1	break					819:823	elongation at break	805:823	elongation at break	805:823	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	5	18	theme	film	842:845	arg1	strength					792:799	tensile strength	784:799	tensile strength	784:799	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	7	19	theme	novel	1180:1184	arg1	materials					1201:1209	novel food packaging materials	1180:1209	novel food packaging materials	1180:1209	The findings contributed to the development of novel food packaging materials.
26794738	0	20	theme	physicochemical	9:23	arg1	properties					25:34	Enhanced physicochemical properties	0:34	Enhanced physicochemical properties of chitosan/whey protein	0:59	Enhanced physicochemical properties of chitosan/whey protein isolate composite film by sodium laurate-modified TiO2 nanoparticles.
26794738	3	21	theme	protein	602:608	arg1	compatibility					580:592	the compatibility	576:592	the compatibility of whey protein isolate and chitosan	576:629	Our results showed that the nanoparticles improved the compatibility of whey protein isolate and chitosan.
26794738	0	22	theme	Enhanced	0:7	arg1	properties					25:34	Enhanced physicochemical properties	0:34	Enhanced physicochemical properties of chitosan/whey protein	0:59	Enhanced physicochemical properties of chitosan/whey protein isolate composite film by sodium laurate-modified TiO2 nanoparticles.
26794738	6	23	theme	water	1034:1038	arg1	isotherm					1049:1056	its water sorption isotherm	1030:1056	its water sorption isotherm of the nanocomposite film	1030:1082	The equilibrium moisture of nanocomposite film was lower than binary film, and its water sorption isotherm of the nanocomposite film fitted well to Guggenheim-Anderson-deBoer model.
26794738	2	24	theme	adsorption	504:513	arg1	isotherm					515:522	water adsorption isotherm	498:522	water adsorption isotherm	498:522	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	7	25	theme	packaging	1191:1199	arg1	materials					1201:1209	novel food packaging materials	1180:1209	novel food packaging materials	1180:1209	The findings contributed to the development of novel food packaging materials.
26794738	5	26	theme	water	902:906	arg1	permeability					914:925	water vapor permeability	902:925	water vapor permeability	902:925	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	2	27	theme	water	498:502	arg1	isotherm					515:522	water adsorption isotherm	498:522	water adsorption isotherm	498:522	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	6	28	theme	film	1079:1082	arg1	isotherm					1049:1056	its water sorption isotherm	1030:1056	its water sorption isotherm of the nanocomposite film	1030:1082	The equilibrium moisture of nanocomposite film was lower than binary film, and its water sorption isotherm of the nanocomposite film fitted well to Guggenheim-Anderson-deBoer model.
26794738	1	29	theme	Chitosan/whey	131:143	arg1	film					161:164	Chitosan/whey protein isolate film	131:164	Chitosan/whey protein isolate film incorporated with sodium laurate-modified TiO2 nanoparticles	131:225	Chitosan/whey protein isolate film incorporated with sodium laurate-modified TiO2 nanoparticles was developed.
26794738	6	30	theme	film	993:996	arg1	lower					1002:1006	lower	1002:1006	lower	1002:1006	The equilibrium moisture of nanocomposite film was lower than binary film, and its water sorption isotherm of the nanocomposite film fitted well to Guggenheim-Anderson-deBoer model.
26794738	6	30	theme	film	993:996	arg1	moisture					967:974	The equilibrium moisture	951:974	The equilibrium moisture of nanocomposite film	951:996	The equilibrium moisture of nanocomposite film was lower than binary film, and its water sorption isotherm of the nanocomposite film fitted well to Guggenheim-Anderson-deBoer model.
26794738	5	31	theme	vapor	908:912	arg1	permeability					914:925	water vapor permeability	902:925	water vapor permeability	902:925	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	0	32	theme	protein	53:59	arg1	properties					25:34	Enhanced physicochemical properties	0:34	Enhanced physicochemical properties of chitosan/whey protein	0:59	Enhanced physicochemical properties of chitosan/whey protein isolate composite film by sodium laurate-modified TiO2 nanoparticles.
26794738	4	33	theme	nanoparticles	644:656	arg1	Addition					632:639	Addition	632:639	Addition of nanoparticles	632:656	Addition of nanoparticles increased the whiteness of chitosan/whey protein isolate film, but decreased its transparency.
26794738	5	34	theme	elongation	805:814	arg1	break					819:823	elongation at break	805:823	elongation at break	805:823	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	6	35	theme	Guggenheim-Anderson-deBoer	1099:1124	arg1	model					1126:1130	Guggenheim-Anderson-deBoer model	1099:1130	Guggenheim-Anderson-deBoer model	1099:1130	The equilibrium moisture of nanocomposite film was lower than binary film, and its water sorption isotherm of the nanocomposite film fitted well to Guggenheim-Anderson-deBoer model.
26794738	2	36	theme	electron	295:302	arg1	microscopy					304:313	scanning electron microscopy	286:313	scanning electron microscopy	286:313	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	2	37	theme	vapor	475:479	arg1	permeability					481:492	water vapor permeability	469:492	water vapor permeability	469:492	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	0	38	theme	chitosan/whey	39:51	arg1	protein					53:59	chitosan/whey protein	39:59	chitosan/whey protein	39:59	Enhanced physicochemical properties of chitosan/whey protein isolate composite film by sodium laurate-modified TiO2 nanoparticles.
26794738	5	39	theme	at	816:817	arg1	break					819:823	elongation at break	805:823	elongation at break	805:823	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	5	40	from	break	819:823	arg1	elongation					805:814	elongation at break	805:823	elongation at break	805:823	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	5	40	from	break	819:823	arg1	at					816:817	elongation at break	805:823	elongation at break	805:823	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	2	41	theme	scanning	286:293	arg1	microscopy					304:313	scanning electron microscopy	286:313	scanning electron microscopy	286:313	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	2	42	theme	water	469:473	arg1	permeability					481:492	water vapor permeability	469:492	water vapor permeability	469:492	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	0	43	theme	composite	69:77	arg1	film					79:82	composite film	69:82	composite film	69:82	Enhanced physicochemical properties of chitosan/whey protein isolate composite film by sodium laurate-modified TiO2 nanoparticles.
26794738	6	44	theme	binary	1013:1018	arg1	film					1020:1023	binary film	1013:1023	binary film	1013:1023	The equilibrium moisture of nanocomposite film was lower than binary film, and its water sorption isotherm of the nanocomposite film fitted well to Guggenheim-Anderson-deBoer model.
26794738	1	45	theme	protein	145:151	arg1	film					161:164	Chitosan/whey protein isolate film	131:164	Chitosan/whey protein isolate film incorporated with sodium laurate-modified TiO2 nanoparticles	131:225	Chitosan/whey protein isolate film incorporated with sodium laurate-modified TiO2 nanoparticles was developed.
26794738	1	46	theme	isolate	153:159	arg1	film					161:164	Chitosan/whey protein isolate film	131:164	Chitosan/whey protein isolate film incorporated with sodium laurate-modified TiO2 nanoparticles	131:225	Chitosan/whey protein isolate film incorporated with sodium laurate-modified TiO2 nanoparticles was developed.
26794738	2	47	from	break	462:466	arg1	isotherm					515:522	water adsorption isotherm	498:522	water adsorption isotherm	498:522	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	2	47	from	break	462:466	arg1	permeability					481:492	water vapor permeability	469:492	water vapor permeability	469:492	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	2	47	from	break	462:466	arg1	elongation					448:457	elongation	448:457	elongation at break	448:466	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	2	47	from	break	462:466	arg1	color					423:427	color	423:427	color	423:427	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	2	47	from	break	462:466	arg1	strength					438:445	tensile strength	430:445	tensile strength	430:445	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	4	48	theme	isolate	707:713	arg1	film					715:718	chitosan/whey protein isolate film	685:718	chitosan/whey protein isolate film	685:718	Addition of nanoparticles increased the whiteness of chitosan/whey protein isolate film, but decreased its transparency.
26794738	5	49	theme	tensile	784:790	arg1	strength					792:799	tensile strength	784:799	tensile strength	784:799	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	2	50	theme	nanocomposite	246:258	arg1	film					260:263	The nanocomposite film	242:263	The nanocomposite film	242:263	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	2	51	theme	X-ray	316:320	arg1	diffraction					322:332	X-ray diffraction	316:332	X-ray diffraction	316:332	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	2	52	theme	physicochemical	393:407	arg1	properties					409:418	physicochemical properties	393:418	physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm	393:522	The nanocomposite film was characterized by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry, and investigated in physicochemical properties as color, tensile strength, elongation at break, water vapor permeability and water adsorption isotherm.
26794738	4	53	theme	protein	699:705	arg1	film					715:718	chitosan/whey protein isolate film	685:718	chitosan/whey protein isolate film	685:718	Addition of nanoparticles increased the whiteness of chitosan/whey protein isolate film, but decreased its transparency.
26794738	3	54	theme	whey	597:600	arg1	protein					602:608	whey protein isolate and chitosan	597:629	protein	602:608	Our results showed that the nanoparticles improved the compatibility of whey protein isolate and chitosan.
26794738	5	55	theme	binary	767:772	arg1	film					774:777	binary film	767:777	binary film	767:777	Compared with binary film, the tensile strength and elongation at break of nanocomposite film were increased by 11.51% and 12.01%, respectively, and water vapor permeability was decreased by 7.60%.
26794738	6	56	theme	nanocomposite	1065:1077	arg1	film					1079:1082	the nanocomposite film	1061:1082	the nanocomposite film	1061:1082	The equilibrium moisture of nanocomposite film was lower than binary film, and its water sorption isotherm of the nanocomposite film fitted well to Guggenheim-Anderson-deBoer model.
26794738	1	57	theme	sodium	184:189	arg1	nanoparticles					213:225	sodium laurate-modified TiO2 nanoparticles	184:225	sodium laurate-modified TiO2 nanoparticles	184:225	Chitosan/whey protein isolate film incorporated with sodium laurate-modified TiO2 nanoparticles was developed.
26794738	4	58	theme	film	715:718	arg1	whiteness					672:680	the whiteness	668:680	the whiteness of chitosan/whey protein isolate film	668:718	Addition of nanoparticles increased the whiteness of chitosan/whey protein isolate film, but decreased its transparency.
29175782	7	0	theme	reference	1507:1515	arg1	formulation					1517:1527	reference formulation	1507:1527	reference formulation of CBZ (F5-X) with similar composition to F7-X	1507:1574	When reference formulation of CBZ (F5-X) with similar composition to F7-X were given to rabbits, cocrystal formulation gave 1.37fold (***P k 0.05) bioavailability than CBZ reference formulation.
29175782	4	1	theme	Polyvinyl	882:890	arg1	®					958:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	1	2	theme	physico-chemical	253:268	arg1	properties					270:279	overall improved physico-chemical properties	236:279	overall improved physico-chemical properties	236:279	Currently cocrystals are considered as an established approach for making crystalline solids with overall improved physico-chemical properties.
29175782	4	3	theme	acetate-polyethylene	914:933	arg1	®					958:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	7	4	with	formulation	1517:1527	arg1	composition					1556:1566	similar composition	1548:1566	similar composition	1548:1566	When reference formulation of CBZ (F5-X) with similar composition to F7-X were given to rabbits, cocrystal formulation gave 1.37fold (***P k 0.05) bioavailability than CBZ reference formulation.
29175782	6	5	theme	graft	1315:1319	arg1	co-polymer®					1321:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	3	6	theme	crystalline	562:572	arg1	drug					574:577	parent crystalline drug	555:577	parent crystalline drug	555:577	The polymeric carriers are long known to manipulate this conversion during dissolution to parent crystalline drug, which may hinder or accelerate the dissolution process if used in a dosage form.
29175782	8	7	theme	formulation	1715:1725	arg1	Cmax					1697:1700	Cmax	1697:1700	Cmax of reference formulation observed	1697:1734	Cmax of reference formulation observed was 3.9μmL-1.
29175782	4	8	theme	study	678:682	arg1	goal					665:668	The goal	661:668	The goal of this study	661:682	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	6	9	theme	acetatepolyethylene	1288:1306	arg1	co-polymer®					1321:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	6	10	theme	formulation	1206:1216	arg1	F7-X					1218:1221	formulation F7-X	1206:1221	formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®)	1206:1332	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	6	11	dep	%	1225:1225	arg1	co-polymer®					1321:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	7	12	theme	***	1636:1638	arg1	k					1641:1641	***P k 0.05	1636:1646	***P k 0.05	1636:1646	When reference formulation of CBZ (F5-X) with similar composition to F7-X were given to rabbits, cocrystal formulation gave 1.37fold (***P k 0.05) bioavailability than CBZ reference formulation.
29175782	6	13	theme	carpolactam-polyvinyl	1266:1286	arg1	co-polymer®					1321:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	5	14	dep	in	1044:1045	arg1	vitro					1047:1051	vitro	1047:1051	vitro	1047:1051	The concentration of these polymers were systemically varied during in vitro dissolution studies, while selected formulations from dissolution studies were tested in vivo.
29175782	0	15	theme	stable	107:112	arg1	formulation					125:135	a stable suspension formulation	105:135	a stable suspension formulation	105:135	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	7	16	theme	1.37fold	1626:1633	arg1	bioavailability					1649:1663	1.37fold (***P k 0.05) bioavailability	1626:1663	1.37fold (***P k 0.05) bioavailability	1626:1663	When reference formulation of CBZ (F5-X) with similar composition to F7-X were given to rabbits, cocrystal formulation gave 1.37fold (***P k 0.05) bioavailability than CBZ reference formulation.
29175782	4	17	theme	graft	942:946	arg1	®					958:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	6	18	theme	unformulated	1458:1469	arg1	neat					1472:1475	unformulated 'neat'	1458:1476	unformulated 'neat' cocrystal	1458:1486	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	4	19	theme	soluble	714:720	arg1	CBZ-SUC					751:757	CBZ-SUC	751:757	CBZ-SUC	751:757	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	4	19	theme	soluble	714:720	arg1	acid					745:748	a more soluble carbamazepine-succinic acid	707:748	a more soluble carbamazepine-succinic acid (CBZ-SUC)	707:758	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	0	20	theme	carbamazepine	45:57	arg1	performance					30:40	Improved in vitro and in vivo performance	0:40	Improved in vitro and in vivo performance of carbamazepine	0:57	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	4	21	from	cocrystal	760:768	arg1	formulation					784:794	suspension formulation	773:794	suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer	773:973	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	4	22	theme	suspension	773:782	arg1	formulation					784:794	suspension formulation	773:794	suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer	773:973	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	7	23	theme	CBZ	1670:1672	arg1	formulation					1684:1694	CBZ reference formulation	1670:1694	CBZ reference formulation	1670:1694	When reference formulation of CBZ (F5-X) with similar composition to F7-X were given to rabbits, cocrystal formulation gave 1.37fold (***P k 0.05) bioavailability than CBZ reference formulation.
29175782	2	24	theme	parent	406:411	arg1	drug					413:416	parent drug	406:416	parent drug	406:416	However, some otherwise well behaving cocrystals undergo rapid dissociation during dissolution, with ultimate conversion to parent drug and thus apparent loss of improved solubility.
29175782	0	25	theme	Improved	0:7	arg1	performance					30:40	Improved in vitro and in vivo performance	0:40	Improved in vitro and in vivo performance of carbamazepine	0:57	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	2	26	theme	solubility	453:462	arg1	drug					413:416	parent drug	406:416	parent drug	406:416	However, some otherwise well behaving cocrystals undergo rapid dissociation during dissolution, with ultimate conversion to parent drug and thus apparent loss of improved solubility.
29175782	2	26	theme	solubility	453:462	arg1	loss					436:439	thus apparent loss	422:439	thus apparent loss of improved solubility	422:462	However, some otherwise well behaving cocrystals undergo rapid dissociation during dissolution, with ultimate conversion to parent drug and thus apparent loss of improved solubility.
29175782	5	27	theme	polymers	1003:1010	arg1	concentration					980:992	The concentration	976:992	The concentration of these polymers	976:1010	The concentration of these polymers were systemically varied during in vitro dissolution studies, while selected formulations from dissolution studies were tested in vivo.
29175782	5	27	theme	polymers	1003:1010	arg1	varied					1030:1035	varied	1030:1035	varied	1030:1035	The concentration of these polymers were systemically varied during in vitro dissolution studies, while selected formulations from dissolution studies were tested in vivo.
29175782	2	28	theme	apparent	427:434	arg1	loss					436:439	thus apparent loss	422:439	thus apparent loss of improved solubility	422:462	However, some otherwise well behaving cocrystals undergo rapid dissociation during dissolution, with ultimate conversion to parent drug and thus apparent loss of improved solubility.
29175782	3	29	theme	polymeric	469:477	arg1	carriers					479:486	The polymeric carriers	465:486	The polymeric carriers	465:486	The polymeric carriers are long known to manipulate this conversion during dissolution to parent crystalline drug, which may hinder or accelerate the dissolution process if used in a dosage form.
29175782	0	30	theme	in	22:23	arg1	performance					30:40	Improved in vitro and in vivo performance	0:40	Improved in vitro and in vivo performance of carbamazepine	0:57	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	6	31	theme	4.73μ.mL-1	1422:1431	arg1	improvement					1354:1364	almost 6fold improvement	1341:1364	almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally	1341:1499	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	6	31	theme	4.73μ.mL-1	1422:1431	arg1	Cmax					1414:1417	much higher Cmax	1402:1417	almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally	1341:1499	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	7	32	theme	CBZ	1532:1534	arg1	formulation					1517:1527	reference formulation	1507:1527	reference formulation of CBZ (F5-X) with similar composition to F7-X	1507:1574	When reference formulation of CBZ (F5-X) with similar composition to F7-X were given to rabbits, cocrystal formulation gave 1.37fold (***P k 0.05) bioavailability than CBZ reference formulation.
29175782	0	33	dep	in	22:23	arg1	vivo					25:28	vivo	25:28	vivo	25:28	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	1	34	theme	crystalline	212:222	arg1	solids					224:229	crystalline solids	212:229	crystalline solids	212:229	Currently cocrystals are considered as an established approach for making crystalline solids with overall improved physico-chemical properties.
29175782	4	35	theme	methyl	820:825	arg1	inhibitor					868:876	a crystallization inhibitor	850:876	a crystallization inhibitor	850:876	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	4	35	theme	methyl	820:825	arg1	HPMC-AS					838:844	Hydroxypropyl methyl cellulose (HPMC-AS)	806:845	Hydroxypropyl methyl cellulose (HPMC-AS)	806:845	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	6	36	theme	6fold	1348:1352	arg1	improvement					1354:1364	almost 6fold improvement	1341:1364	almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally	1341:1499	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	6	37	theme	cocrystal	1478:1486	arg1	neat					1472:1475	unformulated 'neat'	1458:1476	unformulated 'neat' cocrystal	1458:1486	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	1	38	theme	improved	244:251	arg1	properties					270:279	overall improved physico-chemical properties	236:279	overall improved physico-chemical properties	236:279	Currently cocrystals are considered as an established approach for making crystalline solids with overall improved physico-chemical properties.
29175782	6	39	theme	Pharmacokinetic	1148:1162	arg1	PK					1173:1174	PK	1173:1174	PK	1173:1174	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	6	39	theme	Pharmacokinetic	1148:1162	arg1	studies					1164:1170	Pharmacokinetic studies	1148:1170	Pharmacokinetic studies (PK) in rabbits	1148:1186	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	6	40	theme	neat	1472:1475	arg1	1.07μ.mL-1					1444:1453	1.07μ.mL-1	1444:1453	1.07μ.mL-1 of unformulated 'neat' cocrystal given orally	1444:1499	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	4	41	theme	carpolactam-polyvinyl	892:912	arg1	®					958:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	6	42	theme	cocrystal	1227:1235	arg1	%					1225:1225	1% cocrystal	1224:1235	1% cocrystal	1224:1235	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	0	43	from	formulation	125:135	arg1	cocrystal					92:100	cocrystal	92:100	cocrystal	92:100	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	3	44	theme	parent	555:560	arg1	drug					574:577	parent crystalline drug	555:577	parent crystalline drug	555:577	The polymeric carriers are long known to manipulate this conversion during dissolution to parent crystalline drug, which may hinder or accelerate the dissolution process if used in a dosage form.
29175782	6	45	theme	glycol	1308:1313	arg1	co-polymer®					1321:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	8	46	theme	reference	1705:1713	arg1	formulation					1715:1725	reference formulation	1705:1725	reference formulation observed	1705:1734	Cmax of reference formulation observed was 3.9μmL-1.
29175782	7	47	theme	P	1639:1639	arg1	k					1641:1641	***P k 0.05	1636:1646	***P k 0.05	1636:1646	When reference formulation of CBZ (F5-X) with similar composition to F7-X were given to rabbits, cocrystal formulation gave 1.37fold (***P k 0.05) bioavailability than CBZ reference formulation.
29175782	4	48	theme	crystallization	852:866	arg1	inhibitor					868:876	a crystallization inhibitor	850:876	a crystallization inhibitor	850:876	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	4	48	theme	crystallization	852:866	arg1	HPMC-AS					838:844	Hydroxypropyl methyl cellulose (HPMC-AS)	806:845	Hydroxypropyl methyl cellulose (HPMC-AS)	806:845	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	6	49	dep	F7-X	1218:1221	arg1	%					1254:1254	2%	1253:1254	2%	1253:1254	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	6	49	dep	F7-X	1218:1221	arg1	%					1239:1239	1% HPMC-AS	1238:1247	1% HPMC-AS	1238:1247	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	6	49	dep	F7-X	1218:1221	arg1	%					1225:1225	1% cocrystal	1224:1235	1% cocrystal	1224:1235	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	2	50	theme	behaving	311:318	arg1	cocrystals					320:329	some otherwise well behaving cocrystals	291:329	some otherwise well behaving cocrystals	291:329	However, some otherwise well behaving cocrystals undergo rapid dissociation during dissolution, with ultimate conversion to parent drug and thus apparent loss of improved solubility.
29175782	1	51	theme	established	180:190	arg1	approach					192:199	an established approach	177:199	an established approach for making crystalline solids with overall improved physico-chemical properties	177:279	Currently cocrystals are considered as an established approach for making crystalline solids with overall improved physico-chemical properties.
29175782	1	51	theme	established	180:190	arg1	cocrystals					148:157	Currently cocrystals	138:157	Currently cocrystals	138:157	Currently cocrystals are considered as an established approach for making crystalline solids with overall improved physico-chemical properties.
29175782	0	52	theme	suspension	114:123	arg1	formulation					125:135	a stable suspension formulation	105:135	a stable suspension formulation	105:135	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	3	53	theme	dissolution	615:625	arg1	process					627:633	the dissolution process	611:633	the dissolution process	611:633	The polymeric carriers are long known to manipulate this conversion during dissolution to parent crystalline drug, which may hinder or accelerate the dissolution process if used in a dosage form.
29175782	6	54	theme	Polyvinyl	1256:1264	arg1	co-polymer®					1321:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®	1256:1331	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	7	55	theme	cocrystal	1599:1607	arg1	formulation					1609:1619	cocrystal formulation	1599:1619	cocrystal formulation	1599:1619	When reference formulation of CBZ (F5-X) with similar composition to F7-X were given to rabbits, cocrystal formulation gave 1.37fold (***P k 0.05) bioavailability than CBZ reference formulation.
29175782	4	56	theme	glycol	935:940	arg1	®					958:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	0	57	from	cocrystal	92:100	arg1	formulation					125:135	a stable suspension formulation	105:135	a stable suspension formulation	105:135	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	5	58	theme	dissolution	1107:1117	arg1	studies					1119:1125	dissolution studies	1107:1125	dissolution studies	1107:1125	The concentration of these polymers were systemically varied during in vitro dissolution studies, while selected formulations from dissolution studies were tested in vivo.
29175782	4	59	theme	copolymer	948:956	arg1	®					958:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ®	882:958	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	6	60	theme	HPMC-AS	1241:1247	arg1	%					1239:1239	1% HPMC-AS	1238:1247	1% HPMC-AS	1238:1247	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	5	61	from	studies	1119:1125	arg1	formulations					1089:1100	selected formulations	1080:1100	selected formulations from dissolution studies	1080:1125	The concentration of these polymers were systemically varied during in vitro dissolution studies, while selected formulations from dissolution studies were tested in vivo.
29175782	6	62	theme	higher	1407:1412	arg1	Cmax					1414:1417	much higher Cmax	1402:1417	almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally	1341:1499	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	4	63	from	formulation	784:794	arg1	cocrystal					760:768	cocrystal	760:768	cocrystal	760:768	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	0	64	theme	succinic	78:85	arg1	acid					87:90	a succinic acid	76:90	a succinic acid cocrystal in a stable suspension formulation	76:135	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	6	65	from	Cmax	1414:1417	arg1	AUC0-72					1369:1375	AUC0-72	1369:1375	AUC0-72	1369:1375	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	6	65	from	Cmax	1414:1417	arg1	k					1383:1383	***P k 0.05	1378:1388	***P k 0.05	1378:1388	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	0	66	theme	cocrystal	92:100	arg1	acid					87:90	a succinic acid	76:90	a succinic acid cocrystal in a stable suspension formulation	76:135	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	0	67	theme	in	9:10	arg1	performance					30:40	Improved in vitro and in vivo performance	0:40	Improved in vitro and in vivo performance of carbamazepine	0:57	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	7	68	dep	1.37fold	1626:1633	arg1	k					1641:1641	***P k 0.05	1636:1646	***P k 0.05	1636:1646	When reference formulation of CBZ (F5-X) with similar composition to F7-X were given to rabbits, cocrystal formulation gave 1.37fold (***P k 0.05) bioavailability than CBZ reference formulation.
29175782	7	69	theme	reference	1674:1682	arg1	formulation					1684:1694	CBZ reference formulation	1670:1694	CBZ reference formulation	1670:1694	When reference formulation of CBZ (F5-X) with similar composition to F7-X were given to rabbits, cocrystal formulation gave 1.37fold (***P k 0.05) bioavailability than CBZ reference formulation.
29175782	6	70	theme	P	1381:1381	arg1	AUC0-72					1369:1375	AUC0-72	1369:1375	AUC0-72	1369:1375	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	6	70	theme	P	1381:1381	arg1	k					1383:1383	***P k 0.05	1378:1388	***P k 0.05	1378:1388	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	4	71	theme	carbamazepine-succinic	722:743	arg1	CBZ-SUC					751:757	CBZ-SUC	751:757	CBZ-SUC	751:757	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	4	71	theme	carbamazepine-succinic	722:743	arg1	acid					745:748	a more soluble carbamazepine-succinic acid	707:748	a more soluble carbamazepine-succinic acid (CBZ-SUC)	707:758	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	0	72	dep	in	9:10	arg1	vitro					12:16	vitro	12:16	vitro	12:16	Improved in vitro and in vivo performance of carbamazepine enabled by using a succinic acid cocrystal in a stable suspension formulation.
29175782	2	73	theme	improved	444:451	arg1	solubility					453:462	improved solubility	444:462	improved solubility	444:462	However, some otherwise well behaving cocrystals undergo rapid dissociation during dissolution, with ultimate conversion to parent drug and thus apparent loss of improved solubility.
29175782	7	74	theme	similar	1548:1554	arg1	composition					1556:1566	similar composition	1548:1566	similar composition	1548:1566	When reference formulation of CBZ (F5-X) with similar composition to F7-X were given to rabbits, cocrystal formulation gave 1.37fold (***P k 0.05) bioavailability than CBZ reference formulation.
29175782	3	75	theme	dosage	648:653	arg1	form					655:658	a dosage form	646:658	a dosage form	646:658	The polymeric carriers are long known to manipulate this conversion during dissolution to parent crystalline drug, which may hinder or accelerate the dissolution process if used in a dosage form.
29175782	4	76	theme	cellulose	827:835	arg1	inhibitor					868:876	a crystallization inhibitor	850:876	a crystallization inhibitor	850:876	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	4	76	theme	cellulose	827:835	arg1	HPMC-AS					838:844	Hydroxypropyl methyl cellulose (HPMC-AS)	806:845	Hydroxypropyl methyl cellulose (HPMC-AS)	806:845	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	6	77	from	improvement	1354:1364	arg1	AUC0-72					1369:1375	AUC0-72	1369:1375	AUC0-72	1369:1375	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	6	77	from	improvement	1354:1364	arg1	k					1383:1383	***P k 0.05	1378:1388	***P k 0.05	1378:1388	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	5	78	theme	selected	1080:1087	arg1	formulations					1089:1100	selected formulations	1080:1100	selected formulations from dissolution studies	1080:1125	The concentration of these polymers were systemically varied during in vitro dissolution studies, while selected formulations from dissolution studies were tested in vivo.
29175782	5	79	theme	in	1044:1045	arg1	studies					1065:1071	in vitro dissolution studies	1044:1071	in vitro dissolution studies	1044:1071	The concentration of these polymers were systemically varied during in vitro dissolution studies, while selected formulations from dissolution studies were tested in vivo.
29175782	6	80	from	studies	1164:1170	arg1	rabbits					1180:1186	rabbits	1180:1186	rabbits	1180:1186	Pharmacokinetic studies (PK) in rabbits demonstrated that formulation F7-X (1% cocrystal, 1% HPMC-AS and 2% Polyvinyl carpolactam-polyvinyl acetatepolyethylene glycol graft co-polymer®) caused almost 6fold improvement in AUC0-72 (***P k 0.05) as well as much higher Cmax of 4.73μ.mL-1 to that of 1.07μ.mL-1 of unformulated 'neat' cocrystal given orally.
29175782	2	81	theme	rapid	339:343	arg1	dissociation					345:356	rapid dissociation	339:356	rapid dissociation	339:356	However, some otherwise well behaving cocrystals undergo rapid dissociation during dissolution, with ultimate conversion to parent drug and thus apparent loss of improved solubility.
29175782	5	82	theme	dissolution	1053:1063	arg1	studies					1065:1071	in vitro dissolution studies	1044:1071	in vitro dissolution studies	1044:1071	The concentration of these polymers were systemically varied during in vitro dissolution studies, while selected formulations from dissolution studies were tested in vivo.
29175782	4	83	theme	Hydroxypropyl	806:818	arg1	inhibitor					868:876	a crystallization inhibitor	850:876	a crystallization inhibitor	850:876	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	4	83	theme	Hydroxypropyl	806:818	arg1	HPMC-AS					838:844	Hydroxypropyl methyl cellulose (HPMC-AS)	806:845	Hydroxypropyl methyl cellulose (HPMC-AS)	806:845	The goal of this study was to deliver in vivo a more soluble carbamazepine-succinic acid (CBZ-SUC) cocrystal in suspension formulation utilizing Hydroxypropyl methyl cellulose (HPMC-AS) as a crystallization inhibitor and Polyvinyl carpolactam-polyvinyl acetate-polyethylene glycol graft copolymer ® as solubilizer.
29175782	2	84	theme	ultimate	383:390	arg1	conversion					392:401	ultimate conversion	383:401	ultimate conversion to parent drug and thus apparent loss of improved solubility	383:462	However, some otherwise well behaving cocrystals undergo rapid dissociation during dissolution, with ultimate conversion to parent drug and thus apparent loss of improved solubility.
29175782	1	85	theme	overall	236:242	arg1	properties					270:279	overall improved physico-chemical properties	236:279	overall improved physico-chemical properties	236:279	Currently cocrystals are considered as an established approach for making crystalline solids with overall improved physico-chemical properties.
27657815	0	0	theme	strontium	85:93	arg1	removal					74:80	the removal	70:80	the removal of strontium (Sr) from seawater	70:112	Highly stable and magnetically separable alginate/Fe3O4 composite for the removal of strontium (Sr) from seawater.
27657815	7	1	theme	alginate/Fe3O4	1087:1100	arg1	composite					1102:1110	the alginate/Fe3O4 composite	1083:1110	the alginate/Fe3O4 composite	1083:1110	In real seawater spiked with 50 mg/L of Sr, the alginate/Fe3O4 composite showed 12.5 mg/g of Sr uptake, despite the highly concentrated ions in seawater.
27657815	4	2	theme	alginate/Fe3O4	643:656	arg1	composite					658:666	The alginate/Fe3O4 composite	639:666	The alginate/Fe3O4 composite	639:666	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	3	3	theme	Fe3O4	632:636	arg1	sizes					597:601	particle sizes	588:601	particle sizes of <400 μm	588:612	The synthesized composite was optimized for particle sizes of <400 μm and 1% content of Fe3O4.
27657815	3	3	theme	Fe3O4	632:636	arg1	content					621:627	1% content	618:627	1% content of Fe3O4	618:636	The synthesized composite was optimized for particle sizes of <400 μm and 1% content of Fe3O4.
27657815	5	4	theme	Sr	816:817	arg1	50 mg/L					830:836	50 mg/L	830:836	50 mg/L	830:836	However, in diluted Sr condition (50 mg/L), Ca significantly affected Sr adsorption, resulting in a decrease of Kd value from 3.7 to 2.4 at the 0.01 M Ca.
27657815	5	4	theme	Sr	816:817	arg1	condition					819:827	diluted Sr condition	808:827	diluted Sr condition (50 mg/L)	808:837	However, in diluted Sr condition (50 mg/L), Ca significantly affected Sr adsorption, resulting in a decrease of Kd value from 3.7 to 2.4 at the 0.01 M Ca.
27657815	8	5	from	efficiency	1260:1269	arg1	seawater					1286:1293	real seawater	1281:1293	real seawater	1281:1293	The adsorption experiment for radio-active 90Sr revealed a removal efficiency of 67% in real seawater, demonstrating the reliability of the alginate/Fe3O4 composite.
27657815	1	6	theme	radioactive	315:325	arg1	wastewater					327:336	radioactive wastewater	315:336	radioactive wastewater	315:336	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	4	7	dep	cations	768:774	arg1	Ca					785:786	Ca	785:786	Ca	785:786	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	4	7	dep	cations	768:774	arg1	Mg					781:782	Mg	781:782	Mg	781:782	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	4	7	dep	cations	768:774	arg1	Na					777:778	Na	777:778	Na	777:778	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	4	7	dep	cations	768:774	arg1	cations					768:774	various cations	760:774	various cations (Na, Mg, Ca and K)	760:793	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	4	7	dep	cations	768:774	arg1	K					792:792	K	792:792	K	792:792	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	1	8	theme	strontium	253:261	arg1	removal					268:274	strontium (Sr) removal	253:274	strontium (Sr) removal	253:274	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	2	9	theme	rigid	470:474	arg1	structure					476:484	a more rigid structure	463:484	a more rigid structure with little swelling and facile separation	463:527	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	8	10	theme	radio-active	1223:1234	arg1	90Sr					1236:1239	radio-active 90Sr	1223:1239	radio-active 90Sr	1223:1239	The adsorption experiment for radio-active 90Sr revealed a removal efficiency of 67% in real seawater, demonstrating the reliability of the alginate/Fe3O4 composite.
27657815	5	11	theme	diluted	808:814	arg1	50 mg/L					830:836	50 mg/L	830:836	50 mg/L	830:836	However, in diluted Sr condition (50 mg/L), Ca significantly affected Sr adsorption, resulting in a decrease of Kd value from 3.7 to 2.4 at the 0.01 M Ca.
27657815	5	11	theme	diluted	808:814	arg1	condition					819:827	diluted Sr condition	808:827	diluted Sr condition (50 mg/L)	808:837	However, in diluted Sr condition (50 mg/L), Ca significantly affected Sr adsorption, resulting in a decrease of Kd value from 3.7 to 2.4 at the 0.01 M Ca.
27657815	1	12	theme	stable	139:144	arg1	composite					161:169	a highly stable alginate/Fe3O4 composite	130:169	a highly stable alginate/Fe3O4 composite	130:169	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	7	13	theme	Sr	1079:1080	arg1	50 mg/L					1068:1074	50 mg/L	1068:1074	50 mg/L of Sr	1068:1080	In real seawater spiked with 50 mg/L of Sr, the alginate/Fe3O4 composite showed 12.5 mg/g of Sr uptake, despite the highly concentrated ions in seawater.
27657815	8	14	theme	adsorption	1197:1206	arg1	experiment					1208:1217	The adsorption experiment	1193:1217	The adsorption experiment for radio-active 90Sr	1193:1239	The adsorption experiment for radio-active 90Sr revealed a removal efficiency of 67% in real seawater, demonstrating the reliability of the alginate/Fe3O4 composite.
27657815	4	15	theme	outstanding	723:733	arg1	selectivity					735:745	outstanding selectivity	723:745	outstanding selectivity for Sr among various cations (Na, Mg, Ca and K)	723:793	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	1	16	theme	alginate/Fe3O4	146:159	arg1	composite					161:169	a highly stable alginate/Fe3O4 composite	130:169	a highly stable alginate/Fe3O4 composite	130:169	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	0	17	theme	stable	7:12	arg1	alginate/Fe3O4					41:54	Highly stable and magnetically separable alginate/Fe3O4	0:54	Highly stable and magnetically separable alginate/Fe3O4	0:54	Highly stable and magnetically separable alginate/Fe3O4 composite for the removal of strontium (Sr) from seawater.
27657815	2	18	theme	little	491:496	arg1	swelling					498:505	little swelling	491:505	little swelling	491:505	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	1	19	theme	Sr	264:265	arg1	removal					268:274	strontium (Sr) removal	253:274	strontium (Sr) removal	253:274	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	2	20	with	structure	476:484	arg1	swelling					498:505	little swelling	491:505	little swelling	491:505	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	2	20	with	structure	476:484	arg1	separation					518:527	facile separation	511:527	facile separation	511:527	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	8	21	theme	removal	1252:1258	arg1	efficiency					1260:1269	a removal efficiency	1250:1269	a removal efficiency of 67% in real seawater	1250:1293	The adsorption experiment for radio-active 90Sr revealed a removal efficiency of 67% in real seawater, demonstrating the reliability of the alginate/Fe3O4 composite.
27657815	2	22	theme	high	402:405	arg1	contents					407:414	high contents	402:414	high contents of alginic acid and Fe3O4	402:440	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	8	23	theme	real	1281:1284	arg1	seawater					1286:1293	real seawater	1281:1293	real seawater	1281:1293	The adsorption experiment for radio-active 90Sr revealed a removal efficiency of 67% in real seawater, demonstrating the reliability of the alginate/Fe3O4 composite.
27657815	5	24	theme	Kd	908:909	arg1	value					911:915	Kd value	908:915	Kd value	908:915	However, in diluted Sr condition (50 mg/L), Ca significantly affected Sr adsorption, resulting in a decrease of Kd value from 3.7 to 2.4 at the 0.01 M Ca.
27657815	2	25	theme	microspheres	388:399	arg1	use					372:374	the use	368:374	the use of alginate microspheres	368:399	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	1	26	theme	removal	268:274	arg1	application					238:248	the practical application	224:248	the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater	224:336	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	3	27	theme	<400 μm	606:612	arg1	sizes					597:601	particle sizes	588:601	particle sizes of <400 μm	588:612	The synthesized composite was optimized for particle sizes of <400 μm and 1% content of Fe3O4.
27657815	3	27	theme	<400 μm	606:612	arg1	content					621:627	1% content	618:627	1% content of Fe3O4	618:636	The synthesized composite was optimized for particle sizes of <400 μm and 1% content of Fe3O4.
27657815	7	28	theme	Sr	1132:1133	arg1	uptake					1135:1140	Sr uptake	1132:1140	Sr uptake	1132:1140	In real seawater spiked with 50 mg/L of Sr, the alginate/Fe3O4 composite showed 12.5 mg/g of Sr uptake, despite the highly concentrated ions in seawater.
27657815	2	29	theme	alginate	379:386	arg1	microspheres					388:399	alginate microspheres	379:399	alginate microspheres	379:399	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	2	30	theme	Fe3O4	436:440	arg1	contents					407:414	high contents	402:414	high contents of alginic acid and Fe3O4	402:440	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	5	31	theme	Sr	866:867	arg1	adsorption					869:878	Sr adsorption	866:878	Sr adsorption	866:878	However, in diluted Sr condition (50 mg/L), Ca significantly affected Sr adsorption, resulting in a decrease of Kd value from 3.7 to 2.4 at the 0.01 M Ca.
27657815	1	32	from	application	238:248	arg1	media					287:291	complex media	279:291	complex media	279:291	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	1	32	from	application	238:248	arg1	seawater					302:309	seawater	302:309	seawater	302:309	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	1	32	from	application	238:248	arg1	wastewater					327:336	radioactive wastewater	315:336	radioactive wastewater	315:336	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	3	33	theme	synthesized	548:558	arg1	composite					560:568	The synthesized composite	544:568	The synthesized composite	544:568	The synthesized composite was optimized for particle sizes of <400 μm and 1% content of Fe3O4.
27657815	0	34	theme	separable	31:39	arg1	alginate/Fe3O4					41:54	Highly stable and magnetically separable alginate/Fe3O4	0:54	Highly stable and magnetically separable alginate/Fe3O4	0:54	Highly stable and magnetically separable alginate/Fe3O4 composite for the removal of strontium (Sr) from seawater.
27657815	1	35	theme	complex	279:285	arg1	media					287:291	complex media	279:291	complex media	279:291	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	1	35	theme	complex	279:285	arg1	seawater					302:309	seawater	302:309	seawater	302:309	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	1	35	theme	complex	279:285	arg1	wastewater					327:336	radioactive wastewater	315:336	radioactive wastewater	315:336	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
27657815	7	36	theme	real	1042:1045	arg1	seawater					1047:1054	real seawater	1042:1054	real seawater spiked with 50 mg/L of Sr	1042:1080	In real seawater spiked with 50 mg/L of Sr, the alginate/Fe3O4 composite showed 12.5 mg/g of Sr uptake, despite the highly concentrated ions in seawater.
27657815	8	37	theme	composite	1348:1356	arg1	reliability					1314:1324	the reliability	1310:1324	the reliability of the alginate/Fe3O4 composite	1310:1356	The adsorption experiment for radio-active 90Sr revealed a removal efficiency of 67% in real seawater, demonstrating the reliability of the alginate/Fe3O4 composite.
27657815	5	38	theme	value	911:915	arg1	decrease					896:903	a decrease	894:903	a decrease of Kd value	894:915	However, in diluted Sr condition (50 mg/L), Ca significantly affected Sr adsorption, resulting in a decrease of Kd value from 3.7 to 2.4 at the 0.01 M Ca.
27657815	7	39	from	ions	1175:1178	arg1	seawater					1183:1190	seawater	1183:1190	seawater	1183:1190	In real seawater spiked with 50 mg/L of Sr, the alginate/Fe3O4 composite showed 12.5 mg/g of Sr uptake, despite the highly concentrated ions in seawater.
27657815	2	40	theme	use	372:374	arg1	drawbacks					355:363	the drawbacks	351:363	the drawbacks of the use of alginate microspheres	351:399	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	2	41	theme	acid	427:430	arg1	contents					407:414	high contents	402:414	high contents of alginic acid and Fe3O4	402:440	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	8	42	theme	alginate/Fe3O4	1333:1346	arg1	composite					1348:1356	the alginate/Fe3O4 composite	1329:1356	the alginate/Fe3O4 composite	1329:1356	The adsorption experiment for radio-active 90Sr revealed a removal efficiency of 67% in real seawater, demonstrating the reliability of the alginate/Fe3O4 composite.
27657815	2	43	theme	alginic	419:425	arg1	acid					427:430	alginic acid	419:430	alginic acid	419:430	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	5	44	theme	2.4 at	929:934	arg1	0.01 M					940:945	2.4 at the 0.01 M	929:945	2.4 at the 0.01 M	929:945	However, in diluted Sr condition (50 mg/L), Ca significantly affected Sr adsorption, resulting in a decrease of Kd value from 3.7 to 2.4 at the 0.01 M Ca.
27657815	7	45	theme	concentrated	1162:1173	arg1	ions					1175:1178	the highly concentrated ions	1151:1178	the highly concentrated ions in seawater	1151:1190	In real seawater spiked with 50 mg/L of Sr, the alginate/Fe3O4 composite showed 12.5 mg/g of Sr uptake, despite the highly concentrated ions in seawater.
27657815	2	46	used	used	447:450	arg2	contents					407:414	high contents	402:414	high contents of alginic acid and Fe3O4	402:440	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	6	47	dep	0.1 M	1018:1022	arg1	HCl					1024:1026	HCl	1024:1026	HCl	1024:1026	The alginate/Fe3O4 composite could be completely regenerated using 0.1 M HCl and CaCl2.
27657815	8	48	theme	%	1276:1276	arg1	efficiency					1260:1269	a removal efficiency	1250:1269	a removal efficiency of 67% in real seawater	1250:1293	The adsorption experiment for radio-active 90Sr revealed a removal efficiency of 67% in real seawater, demonstrating the reliability of the alginate/Fe3O4 composite.
27657815	4	49	theme	Sr	685:686	arg1	≈400.0 mg/g					696:706	≈400.0 mg/g	696:706	≈400.0 mg/g	696:706	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	4	49	theme	Sr	685:686	arg1	uptake					688:693	excellent Sr uptake	675:693	excellent Sr uptake (≈400.0 mg/g)	675:707	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	2	50	theme	facile	511:516	arg1	separation					518:527	facile separation	511:527	facile separation	511:527	To overcome the drawbacks of the use of alginate microspheres, high contents of alginic acid and Fe3O4 were used to provide a more rigid structure with little swelling and facile separation, respectively.
27657815	3	51	theme	1	618:618	arg1	%					619:619	%	619:619	%	619:619	The synthesized composite was optimized for particle sizes of <400 μm and 1% content of Fe3O4.
27657815	3	52	theme	particle	588:595	arg1	sizes					597:601	particle sizes	588:601	particle sizes of <400 μm	588:612	The synthesized composite was optimized for particle sizes of <400 μm and 1% content of Fe3O4.
27657815	4	53	theme	excellent	675:683	arg1	≈400.0 mg/g					696:706	≈400.0 mg/g	696:706	≈400.0 mg/g	696:706	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	4	53	theme	excellent	675:683	arg1	uptake					688:693	excellent Sr uptake	675:693	excellent Sr uptake (≈400.0 mg/g)	675:707	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	3	54	theme	%	619:619	arg1	content					621:627	1% content	618:627	1% content of Fe3O4	618:636	The synthesized composite was optimized for particle sizes of <400 μm and 1% content of Fe3O4.
27657815	4	55	theme	various	760:766	arg1	Ca					785:786	Ca	785:786	Ca	785:786	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	4	55	theme	various	760:766	arg1	Mg					781:782	Mg	781:782	Mg	781:782	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	4	55	theme	various	760:766	arg1	Na					777:778	Na	777:778	Na	777:778	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	4	55	theme	various	760:766	arg1	K					792:792	K	792:792	K	792:792	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	4	55	theme	various	760:766	arg1	cations					768:774	various cations	760:774	various cations (Na, Mg, Ca and K)	760:793	The alginate/Fe3O4 composite showed excellent Sr uptake (≈400.0 mg/g) and exhibited outstanding selectivity for Sr among various cations (Na, Mg, Ca and K).
27657815	7	56	theme	uptake	1135:1140	arg1	12.5 mg/g					1119:1127	12.5 mg/g	1119:1127	12.5 mg/g of Sr uptake	1119:1140	In real seawater spiked with 50 mg/L of Sr, the alginate/Fe3O4 composite showed 12.5 mg/g of Sr uptake, despite the highly concentrated ions in seawater.
27657815	6	57	theme	alginate/Fe3O4	955:968	arg1	composite					970:978	The alginate/Fe3O4 composite	951:978	The alginate/Fe3O4 composite	951:978	The alginate/Fe3O4 composite could be completely regenerated using 0.1 M HCl and CaCl2.
27657815	0	58	from	seawater	105:112	arg1	removal					74:80	the removal	70:80	the removal of strontium (Sr) from seawater	70:112	Highly stable and magnetically separable alginate/Fe3O4 composite for the removal of strontium (Sr) from seawater.
27657815	5	59	dep	affected	857:864	arg1	Ca.					947:949	Ca.	947:949	Ca.	947:949	However, in diluted Sr condition (50 mg/L), Ca significantly affected Sr adsorption, resulting in a decrease of Kd value from 3.7 to 2.4 at the 0.01 M Ca.
27657815	1	60	theme	practical	228:236	arg1	application					238:248	the practical application	224:248	the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater	224:336	In this study, a highly stable alginate/Fe3O4 composite was synthesized, and systematically investigated for the practical application of strontium (Sr) removal in complex media, such as seawater and radioactive wastewater.
28039824	0	0	theme	enzyme	104:109	arg1	activity					111:118	lignocellulolytic enzyme activity	86:118	lignocellulolytic enzyme activity	86:118	Design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity.
28039824	0	1	theme	lignocellulolytic	86:102	arg1	activity					111:118	lignocellulolytic enzyme activity	86:118	lignocellulolytic enzyme activity	86:118	Design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity.
28039824	3	2	theme	above	675:679	arg1	strains					681:687	the above strains	671:687	the above strains	671:687	However, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strains, it engendered more microbial interactions with the above strains and universally improved the β-glucosidase activity of the consortia.
28039824	5	3	from	consortia	964:972	arg1	fungal					926:931	fungal	926:931	fungal	926:931	One fungal and 16 bacterial genera in the consortia may interact with T. reesei and are potential members of a devised synergistic microbial consortium.
28039824	5	4	theme	microbial	1053:1061	arg1	consortium					1063:1072	a devised synergistic microbial consortium	1031:1072	a devised synergistic microbial consortium	1031:1072	One fungal and 16 bacterial genera in the consortia may interact with T. reesei and are potential members of a devised synergistic microbial consortium.
28039824	3	5	with	community	525:533	arg1	capacity					553:560	cellulolytic capacity	540:560	cellulolytic capacity	540:560	However, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strains, it engendered more microbial interactions with the above strains and universally improved the β-glucosidase activity of the consortia.
28039824	4	6	from	bacteria	823:830	arg1	consortia					839:847	the consortia	835:847	the consortia	835:847	Analysis of the microbial community structure revealed that the bacteria in the consortia are more important for lignocellulolytic enzyme activity than the fungi.
28039824	0	7	theme	microbial	53:61	arg1	consortia					63:71	synthetic fungal-bacterial microbial consortia	26:71	synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity	26:118	Design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity.
28039824	2	8	theme	consortia	305:313	arg1	series					273:278	a series	271:278	a series of synergistic microbial consortia of increasing diversity and complexity	271:352	To improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including Trichoderma reesei, Penicillium decumbens, Aspergillus tubingensis, and Aspergillus niger.
28039824	3	9	theme	strains	615:621	arg1	number					605:610	the number	601:610	the number of strains	601:621	However, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strains, it engendered more microbial interactions with the above strains and universally improved the β-glucosidase activity of the consortia.
28039824	1	10	theme	metabolic	203:211	arg1	efficiency					213:222	metabolic efficiency	203:222	metabolic efficiency	203:222	Microbial interactions are important for metabolism as they can improve or reduce metabolic efficiency.
28039824	6	11	theme	microbial	1088:1096	arg1	consortia					1098:1106	Such devised microbial consortia	1075:1106	Such devised microbial consortia	1075:1106	Such devised microbial consortia may potentially be applied to effectively and economically degrade lignocellulose.
28039824	4	12	theme	structure	795:803	arg1	Analysis					759:766	Analysis	759:766	Analysis of the microbial community structure	759:803	Analysis of the microbial community structure revealed that the bacteria in the consortia are more important for lignocellulolytic enzyme activity than the fungi.
28039824	5	13	theme	bacterial	940:948	arg1	genera					950:955	16 bacterial genera	937:955	16 bacterial genera	937:955	One fungal and 16 bacterial genera in the consortia may interact with T. reesei and are potential members of a devised synergistic microbial consortium.
28039824	1	14	theme	Microbial	121:129	arg1	interactions					131:142	Microbial interactions	121:142	Microbial interactions	121:142	Microbial interactions are important for metabolism as they can improve or reduce metabolic efficiency.
28039824	4	15	theme	enzyme	890:895	arg1	activity					897:904	lignocellulolytic enzyme activity	872:904	lignocellulolytic enzyme activity than the fungi	872:919	Analysis of the microbial community structure revealed that the bacteria in the consortia are more important for lignocellulolytic enzyme activity than the fungi.
28039824	3	16	theme	screened	506:513	arg1	community					525:533	a screened microbial community	504:533	a screened microbial community with cellulolytic capacity	504:560	However, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strains, it engendered more microbial interactions with the above strains and universally improved the β-glucosidase activity of the consortia.
28039824	5	17	theme	potential	1010:1018	arg1	members					1020:1026	potential members	1010:1026	potential members of a devised synergistic microbial consortium	1010:1072	One fungal and 16 bacterial genera in the consortia may interact with T. reesei and are potential members of a devised synergistic microbial consortium.
28039824	3	18	theme	microbial	515:523	arg1	community					525:533	a screened microbial community	504:533	a screened microbial community with cellulolytic capacity	504:560	However, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strains, it engendered more microbial interactions with the above strains and universally improved the β-glucosidase activity of the consortia.
28039824	0	19	theme	fungal-bacterial	36:51	arg1	consortia					63:71	synthetic fungal-bacterial microbial consortia	26:71	synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity	26:118	Design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity.
28039824	3	20	theme	more	638:641	arg1	interactions					653:664	more microbial interactions	638:664	more microbial interactions	638:664	However, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strains, it engendered more microbial interactions with the above strains and universally improved the β-glucosidase activity of the consortia.
28039824	0	21	theme	synthetic	26:34	arg1	consortia					63:71	synthetic fungal-bacterial microbial consortia	26:71	synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity	26:118	Design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity.
28039824	3	22	theme	microbial	643:651	arg1	interactions					653:664	more microbial interactions	638:664	more microbial interactions	638:664	However, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strains, it engendered more microbial interactions with the above strains and universally improved the β-glucosidase activity of the consortia.
28039824	0	23	theme	consortia	63:71	arg1	composition					11:21	composition	11:21	composition	11:21	Design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity.
28039824	0	23	theme	consortia	63:71	arg1	Design					0:5	Design	0:5	Design	0:5	Design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity.
28039824	3	24	theme	cellulolytic	540:551	arg1	capacity					553:560	cellulolytic capacity	540:560	cellulolytic capacity	540:560	However, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strains, it engendered more microbial interactions with the above strains and universally improved the β-glucosidase activity of the consortia.
28039824	3	25	theme	consortia	748:756	arg1	activity					732:739	the β-glucosidase activity	714:739	the β-glucosidase activity of the consortia	714:756	However, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strains, it engendered more microbial interactions with the above strains and universally improved the β-glucosidase activity of the consortia.
28039824	2	26	theme	lignocellulolytic	236:252	arg1	activity					261:268	lignocellulolytic enzyme activity	236:268	lignocellulolytic enzyme activity	236:268	To improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including Trichoderma reesei, Penicillium decumbens, Aspergillus tubingensis, and Aspergillus niger.
28039824	2	27	theme	enzyme	254:259	arg1	activity					261:268	lignocellulolytic enzyme activity	236:268	lignocellulolytic enzyme activity	236:268	To improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including Trichoderma reesei, Penicillium decumbens, Aspergillus tubingensis, and Aspergillus niger.
28039824	5	28	theme	synergistic	1041:1051	arg1	consortium					1063:1072	a devised synergistic microbial consortium	1031:1072	a devised synergistic microbial consortium	1031:1072	One fungal and 16 bacterial genera in the consortia may interact with T. reesei and are potential members of a devised synergistic microbial consortium.
28039824	2	29	theme	microbial	295:303	arg1	consortia					305:313	synergistic microbial consortia	283:313	synergistic microbial consortia of increasing diversity and complexity	283:352	To improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including Trichoderma reesei, Penicillium decumbens, Aspergillus tubingensis, and Aspergillus niger.
28039824	4	30	theme	lignocellulolytic	872:888	arg1	activity					897:904	lignocellulolytic enzyme activity	872:904	lignocellulolytic enzyme activity than the fungi	872:919	Analysis of the microbial community structure revealed that the bacteria in the consortia are more important for lignocellulolytic enzyme activity than the fungi.
28039824	2	31	theme	fungal	373:378	arg1	strains					380:386	fungal strains	373:386	fungal strains	373:386	To improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including Trichoderma reesei, Penicillium decumbens, Aspergillus tubingensis, and Aspergillus niger.
28039824	2	31	theme	fungal	373:378	arg1	niger					483:487	Aspergillus niger	471:487	Aspergillus niger	471:487	To improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including Trichoderma reesei, Penicillium decumbens, Aspergillus tubingensis, and Aspergillus niger.
28039824	2	31	theme	fungal	373:378	arg1	reesei					411:416	Trichoderma reesei	399:416	Trichoderma reesei	399:416	To improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including Trichoderma reesei, Penicillium decumbens, Aspergillus tubingensis, and Aspergillus niger.
28039824	2	31	theme	fungal	373:378	arg1	decumbens					431:439	Penicillium decumbens	419:439	Penicillium decumbens	419:439	To improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including Trichoderma reesei, Penicillium decumbens, Aspergillus tubingensis, and Aspergillus niger.
28039824	2	31	theme	fungal	373:378	arg1	tubingensis					454:464	Aspergillus tubingensis	442:464	Aspergillus tubingensis	442:464	To improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including Trichoderma reesei, Penicillium decumbens, Aspergillus tubingensis, and Aspergillus niger.
28039824	5	32	theme	devised	1033:1039	arg1	consortium					1063:1072	a devised synergistic microbial consortium	1031:1072	a devised synergistic microbial consortium	1031:1072	One fungal and 16 bacterial genera in the consortia may interact with T. reesei and are potential members of a devised synergistic microbial consortium.
28039824	2	33	theme	synergistic	283:293	arg1	consortia					305:313	synergistic microbial consortia	283:313	synergistic microbial consortia of increasing diversity and complexity	283:352	To improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including Trichoderma reesei, Penicillium decumbens, Aspergillus tubingensis, and Aspergillus niger.
28039824	4	34	theme	microbial	775:783	arg1	structure					795:803	the microbial community structure	771:803	the microbial community structure	771:803	Analysis of the microbial community structure revealed that the bacteria in the consortia are more important for lignocellulolytic enzyme activity than the fungi.
28039824	4	35	theme	community	785:793	arg1	structure					795:803	the microbial community structure	771:803	the microbial community structure	771:803	Analysis of the microbial community structure revealed that the bacteria in the consortia are more important for lignocellulolytic enzyme activity than the fungi.
28039824	3	36	theme	β-glucosidase	718:730	arg1	activity					732:739	the β-glucosidase activity	714:739	the β-glucosidase activity of the consortia	714:756	However, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strains, it engendered more microbial interactions with the above strains and universally improved the β-glucosidase activity of the consortia.
28039824	5	37	theme	consortium	1063:1072	arg1	members					1020:1026	potential members	1010:1026	potential members of a devised synergistic microbial consortium	1010:1072	One fungal and 16 bacterial genera in the consortia may interact with T. reesei and are potential members of a devised synergistic microbial consortium.
28039824	6	38	theme	devised	1080:1086	arg1	consortia					1098:1106	Such devised microbial consortia	1075:1106	Such devised microbial consortia	1075:1106	Such devised microbial consortia may potentially be applied to effectively and economically degrade lignocellulose.
28039824	5	39	from	fungal	926:931	arg1	consortia					964:972	the consortia	960:972	the consortia	960:972	One fungal and 16 bacterial genera in the consortia may interact with T. reesei and are potential members of a devised synergistic microbial consortium.
28039824	6	40	theme	Such	1075:1078	arg1	consortia					1098:1106	Such devised microbial consortia	1075:1106	Such devised microbial consortia	1075:1106	Such devised microbial consortia may potentially be applied to effectively and economically degrade lignocellulose.
26256178	0	0	theme	antimicrobial	75:87	arg1	activity					89:96	its antimicrobial activity	71:96	its antimicrobial activity against Esherichia coli	71:120	Correlation between the sorption of dissolved oxygen onto chitosan and its antimicrobial activity against Esherichia coli.
26256178	6	1	theme	activity	886:893	arg1	sources					861:867	the sources	857:867	the sources of antimicrobial activity of chitosan	857:905	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	4	2	theme	additional	531:540	arg1	treatment					542:550	The additional treatment	527:550	The additional treatment of chitosan with air oxygen or electrochemically produced oxygen	527:615	The additional treatment of chitosan with air oxygen or electrochemically produced oxygen also increases the uptake of dissolved oxygen by chitosan.
26256178	6	3	theme	antimicrobial	872:884	arg1	activity					886:893	antimicrobial activity	872:893	antimicrobial activity of chitosan	872:905	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	1	4	theme	dissolved	157:165	arg1	oxygen					167:172	dissolved oxygen	157:172	dissolved oxygen	157:172	The ability of chitosan to adsorb dissolved oxygen from solution depends on its physical shape and is related to the surface area.
26256178	1	5	theme	surface	240:246	arg1	area					248:251	the surface area	236:251	the surface area	236:251	The ability of chitosan to adsorb dissolved oxygen from solution depends on its physical shape and is related to the surface area.
26256178	6	6	theme	sources	861:867	arg1	ability					914:920	the ability	910:920	the ability to sorb dissolved oxygen	910:945	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	6	6	theme	sources	861:867	arg1	one					850:852	one	850:852	one	850:852	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	6	6	theme	sources	861:867	arg1	sources					861:867	the sources	857:867	the sources of antimicrobial activity of chitosan	857:905	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	4	7	theme	oxygen	656:661	arg1	uptake					636:641	the uptake	632:641	the uptake of dissolved oxygen by chitosan	632:673	The additional treatment of chitosan with air oxygen or electrochemically produced oxygen also increases the uptake of dissolved oxygen by chitosan.
26256178	5	8	theme	antimicrobial	757:769	arg1	activity					771:778	its antimicrobial activity	753:778	its antimicrobial activity against Esherichia coli	753:802	A strong correlation between the amount of oxygen adsorbed onto chitosan and its antimicrobial activity against Esherichia coli has been observed.
26256178	6	9	theme	other	959:963	arg1	factors					976:982	other well-known factors	959:982	other well-known factors such as physical state and chemical composition	959:1030	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	6	9	theme	other	959:963	arg1	state					1001:1005	physical state	992:1005	physical state	992:1005	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	6	9	theme	other	959:963	arg1	composition					1020:1030	chemical composition	1011:1030	chemical composition	1011:1030	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	3	10	theme	Pd	430:431	arg1	ions					437:440	Pd(II) ions	430:440	Pd(II) ions	430:440	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	3	11	theme	succinic	415:422	arg1	acid					424:427	succinic acid	415:427	succinic acid	415:427	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	3	12	theme	antimicrobial	383:395	arg1	activity					397:404	antimicrobial activity	383:404	antimicrobial activity	383:404	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	3	13	contain	possessing	372:381	arg1	acid					424:427	succinic acid	415:427	succinic acid	415:427	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	3	13	contain	possessing	372:381	arg2	activity					397:404	antimicrobial activity	383:404	antimicrobial activity	383:404	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	3	13	contain	possessing	372:381	arg1	Pd					452:453	metallic Pd	443:453	metallic Pd	443:453	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	3	13	contain	possessing	372:381	arg1	substances					361:370	the substances	357:370	the substances	357:370	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	3	13	contain	possessing	372:381	arg1	Ag					458:459	Ag	458:459	Ag	458:459	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	3	13	contain	possessing	372:381	arg1	ions					437:440	Pd(II) ions	430:440	Pd(II) ions	430:440	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	6	14	theme	chemical	1011:1018	arg1	composition					1020:1030	chemical composition	1011:1030	chemical composition	1011:1030	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	3	15	theme	dissolved	509:517	arg1	oxygen					519:524	the dissolved oxygen	505:524	the dissolved oxygen	505:524	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	1	16	theme	chitosan	138:145	arg1	ability					127:133	The ability	123:133	The ability of chitosan to adsorb dissolved oxygen from solution	123:186	The ability of chitosan to adsorb dissolved oxygen from solution depends on its physical shape and is related to the surface area.
26256178	1	16	theme	chitosan	138:145	arg1	related					225:231	related	225:231	related	225:231	The ability of chitosan to adsorb dissolved oxygen from solution depends on its physical shape and is related to the surface area.
26256178	0	17	theme	dissolved	36:44	arg1	oxygen					46:51	dissolved oxygen	36:51	dissolved oxygen	36:51	Correlation between the sorption of dissolved oxygen onto chitosan and its antimicrobial activity against Esherichia coli.
26256178	1	18	theme	physical	203:210	arg1	shape					212:216	its physical shape	199:216	its physical shape	199:216	The ability of chitosan to adsorb dissolved oxygen from solution depends on its physical shape and is related to the surface area.
26256178	5	19	theme	strong	678:683	arg1	correlation					685:695	A strong correlation	676:695	A strong correlation between the amount of oxygen adsorbed onto chitosan and its antimicrobial activity against Esherichia coli	676:802	A strong correlation between the amount of oxygen adsorbed onto chitosan and its antimicrobial activity against Esherichia coli has been observed.
26256178	3	20	theme	II	433:434	arg1	ions					437:440	Pd(II) ions	430:440	Pd(II) ions	430:440	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	6	21	theme	physical	992:999	arg1	state					1001:1005	physical state	992:1005	physical state	992:1005	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	0	22	theme	oxygen	46:51	arg1	sorption					24:31	the sorption	20:31	the sorption of dissolved oxygen onto chitosan	20:65	Correlation between the sorption of dissolved oxygen onto chitosan and its antimicrobial activity against Esherichia coli.
26256178	6	23	theme	chitosan	898:905	arg1	activity					886:893	antimicrobial activity	872:893	antimicrobial activity of chitosan	872:905	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	4	24	dep	air	569:571	arg1	oxygen					573:578	oxygen	573:578	oxygen	573:578	The additional treatment of chitosan with air oxygen or electrochemically produced oxygen also increases the uptake of dissolved oxygen by chitosan.
26256178	4	25	with	treatment	542:550	arg1	oxygen					610:615	electrochemically produced oxygen	583:615	electrochemically produced oxygen	583:615	The additional treatment of chitosan with air oxygen or electrochemically produced oxygen also increases the uptake of dissolved oxygen by chitosan.
26256178	4	25	with	treatment	542:550	arg1	air					569:571	air oxygen or electrochemically produced oxygen	569:615	air	569:571	The additional treatment of chitosan with air oxygen or electrochemically produced oxygen also increases the uptake of dissolved oxygen by chitosan.
26256178	3	26	theme	metallic	443:450	arg1	Pd					452:453	metallic Pd	443:453	metallic Pd	443:453	Chitosan, modificated by the substances possessing antimicrobial activity, such as succinic acid, Pd(II) ions, metallic Pd or Ag, distinctly increases the ability to adsorb the dissolved oxygen.
26256178	4	27	theme	chitosan	555:562	arg1	treatment					542:550	The additional treatment	527:550	The additional treatment of chitosan with air oxygen or electrochemically produced oxygen	527:615	The additional treatment of chitosan with air oxygen or electrochemically produced oxygen also increases the uptake of dissolved oxygen by chitosan.
26256178	4	28	theme	produced	601:608	arg1	oxygen					610:615	electrochemically produced oxygen	583:615	electrochemically produced oxygen	583:615	The additional treatment of chitosan with air oxygen or electrochemically produced oxygen also increases the uptake of dissolved oxygen by chitosan.
26256178	4	29	theme	dissolved	646:654	arg1	oxygen					656:661	dissolved oxygen	646:661	dissolved oxygen	646:661	The additional treatment of chitosan with air oxygen or electrochemically produced oxygen also increases the uptake of dissolved oxygen by chitosan.
26256178	5	30	theme	oxygen	719:724	arg1	oxygen					719:724	oxygen	719:724	oxygen adsorbed onto chitosan	719:747	A strong correlation between the amount of oxygen adsorbed onto chitosan and its antimicrobial activity against Esherichia coli has been observed.
26256178	5	30	theme	oxygen	719:724	arg1	amount					709:714	the amount	705:714	the amount of oxygen adsorbed onto chitosan	705:747	A strong correlation between the amount of oxygen adsorbed onto chitosan and its antimicrobial activity against Esherichia coli has been observed.
26256178	5	30	theme	oxygen	719:724	arg1	activity					771:778	its antimicrobial activity	753:778	its antimicrobial activity against Esherichia coli	753:802	A strong correlation between the amount of oxygen adsorbed onto chitosan and its antimicrobial activity against Esherichia coli has been observed.
26256178	6	31	theme	well-known	965:974	arg1	factors					976:982	other well-known factors	959:982	other well-known factors such as physical state and chemical composition	959:1030	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	6	31	theme	well-known	965:974	arg1	state					1001:1005	physical state	992:1005	physical state	992:1005	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26256178	6	31	theme	well-known	965:974	arg1	composition					1020:1030	chemical composition	1011:1030	chemical composition	1011:1030	This finding suggests that one of the sources of antimicrobial activity of chitosan is the ability to sorb dissolved oxygen, along with other well-known factors such as physical state and chemical composition.
26905884	3	0	theme	phosphate	1107:1115	arg1	pH					1125:1126	pH 6.5	1125:1130	pH 6.5	1125:1130	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	0	theme	phosphate	1107:1115	arg1	buffer					1117:1122	the 10mM sodium dihydrogen phosphate buffer	1080:1122	the 10mM sodium dihydrogen phosphate buffer	1080:1122	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	1	theme	new	940:942	arg1	ion-exchanger					967:979	this new temperature-responsive ion-exchanger	935:979	this new temperature-responsive ion-exchanger	935:979	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	5	2	theme	similar	1720:1726	arg1	conditions					1728:1737	similar conditions	1720:1737	similar conditions of changing temperature	1720:1761	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	2	3	contain	containing	526:535	arg2	w/w					573:575	w/w	573:575	w/w	573:575	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	3	contain	containing	526:535	arg2	%					570:570	5%	569:570	5% (w/w)	569:576	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	3	contain	containing	526:535	arg1	N-iso-propylacrylamide					503:524	N-iso-propylacrylamide	503:524	N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid	503:589	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	3	contain	containing	526:535	arg2	N-phenylacrylamide					546:563	5% (w/w) N-phenylacrylamide	537:563	5% (w/w) N-phenylacrylamide	537:563	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	4	theme	bovine	741:746	arg1	resin					763:767	62 mg bovine lactoferrin/mL resin	735:767	62 mg bovine lactoferrin/mL resin	735:767	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	5	5	theme	bovine	1793:1798	arg1	β-lactoglobulin					1800:1814	bovine β-lactoglobulin	1793:1814	bovine β-lactoglobulin	1793:1814	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	5	5	theme	bovine	1793:1798	arg1	protein					1784:1790	the anionic protein	1772:1790	the anionic protein	1772:1790	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	3	6	theme	flow	1140:1143	arg1	rate					1145:1148	the flow rate	1136:1148	the flow rate	1136:1148	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	7	from	%	838:838	arg1	protein					892:898	a normalised mg protein	876:898	a normalised mg protein per mL resin basis	876:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	4	8	with	resins	1211:1216	arg1	lactoferrin					1230:1240	bovine lactoferrin	1223:1240	bovine lactoferrin	1223:1240	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	4	9	theme	optimal	1565:1571	arg1	desorption/adsorption					1573:1593	optimal desorption/adsorption	1565:1593	optimal desorption/adsorption	1565:1593	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	2	10	dep	resin	717:721	arg1	e.g.					684:687	e.g.	684:687	e.g.	684:687	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	11	theme	acrylic	578:584	arg1	acid					586:589	acrylic acid	578:589	acrylic acid	578:589	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	3	12	from	protein	892:898	arg1	%					838:838	94%	836:838	94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis	836:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	12	from	protein	892:898	arg1	lactoferrin					861:871	the loaded bovine lactoferrin	843:871	the loaded bovine lactoferrin on a normalised mg protein per mL resin basis	843:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	5	13	theme	bovine	1618:1623	arg1	lactoperoxidase					1625:1639	bovine lactoperoxidase	1618:1639	bovine lactoperoxidase	1618:1639	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	5	13	theme	bovine	1618:1623	arg1	protein					1609:1615	The cationic protein	1596:1615	The cationic protein	1596:1615	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	2	14	theme	adsorption	615:624	arg1	Bmax					636:639	Bmax	636:639	Bmax	636:639	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	14	theme	adsorption	615:624	arg1	capacity					626:633	the highest adsorption capacity	603:633	the highest adsorption capacity	603:633	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	1	15	theme	temperature-responsive	116:137	arg1	resins					152:157	temperature-responsive ion exchange resins	116:157	temperature-responsive ion exchange resins of different polymer composition	116:190	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	2	16	theme	lactoferrin/mL	702:715	arg1	resin					717:721	14 mg bovine lactoferrin/mL resin	689:721	14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C	689:776	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	3	17	theme	dynamic	799:805	arg1	conditions					815:824	dynamic loading conditions	799:824	dynamic loading conditions at 40 °C	799:833	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	1	18	theme	exchange	143:150	arg1	resins					152:157	temperature-responsive ion exchange resins	116:157	temperature-responsive ion exchange resins of different polymer composition	116:190	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	2	19	theme	62	735:736	arg1	mg					738:739	mg	738:739	mg	738:739	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	0	20	theme	polymers	66:73	arg1	application					15:25	the application	11:25	the application of temperature-responsive ion exchange polymers with whey proteins	11:92	Studies on the application of temperature-responsive ion exchange polymers with whey proteins.
26905884	5	21	theme	temperature	1855:1865	arg1	conditions					1867:1876	temperature conditions	1855:1876	temperature conditions	1855:1876	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	4	22	theme	dynamic	1424:1430	arg1	capture					1432:1438	the corresponding dynamic capture	1406:1438	the corresponding dynamic capture	1406:1438	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	3	23	theme	bovine	854:859	arg1	lactoferrin					861:871	the loaded bovine lactoferrin	843:871	the loaded bovine lactoferrin on a normalised mg protein per mL resin basis	843:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	0	24	theme	whey	80:83	arg1	proteins					85:92	whey proteins	80:92	whey proteins	80:92	Studies on the application of temperature-responsive ion exchange polymers with whey proteins.
26905884	1	25	theme	polymer	172:178	arg1	composition					180:190	different polymer composition	162:190	different polymer composition	162:190	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	0	26	from	Studies	0:6	arg1	application					15:25	the application	11:25	the application of temperature-responsive ion exchange polymers with whey proteins	11:92	Studies on the application of temperature-responsive ion exchange polymers with whey proteins.
26905884	4	27	theme	binding	1155:1161	arg1	characteristics					1163:1177	The binding characteristics	1151:1177	The binding characteristics of these different ion exchange resins with bovine lactoferrin	1151:1240	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	2	28	dep	%	538:538	arg1	w/w					541:543	w/w	541:543	w/w	541:543	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	3	29	theme	normalised	878:887	arg1	protein					892:898	a normalised mg protein	876:898	a normalised mg protein per mL resin basis	876:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	2	30	theme	adsorption	447:456	arg1	conditions					458:467	batch adsorption conditions	441:467	batch adsorption conditions	441:467	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	4	31	theme	corresponding	1410:1422	arg1	capture					1432:1438	the corresponding dynamic capture	1406:1438	the corresponding dynamic capture	1406:1438	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	2	32	theme	mg	692:693	arg1	resin					717:721	14 mg bovine lactoferrin/mL resin	689:721	14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C	689:776	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	4	33	theme	temperature	1505:1515	arg1	conditions					1517:1526	different temperature conditions	1495:1526	different temperature conditions of 20 °C and 50 °C	1495:1545	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	1	34	theme	acrylic	313:319	arg1	derivatives					326:336	acrylic acid derivatives	313:336	acrylic acid derivatives	313:336	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	1	35	theme	Several	95:101	arg1	types					107:111	Several new types	95:111	Several new types of temperature-responsive ion exchange resins of different polymer composition	95:190	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	3	36	theme	mL	904:905	arg1	basis					913:917	mL resin basis	904:917	mL resin basis	904:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	1	37	theme	derivatives	326:336	arg1	co-polymerisation					245:261	the co-polymerisation	241:261	the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose	241:362	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	4	38	theme	bovine	1467:1472	arg1	lactoferrin					1474:1484	bovine lactoferrin	1467:1484	bovine lactoferrin	1467:1484	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	1	39	theme	cross-linked	343:354	arg1	agarose					356:362	cross-linked agarose	343:362	cross-linked agarose	343:362	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	2	40	theme	5	537:537	arg1	%					538:538	%	538:538	%	538:538	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	41	theme	isotherms	389:397	arg1	Analysis					365:372	Analysis	365:372	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions	365:467	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	0	42	theme	ion	53:55	arg1	polymers					66:73	temperature-responsive ion exchange polymers	30:73	temperature-responsive ion exchange polymers with whey proteins	30:92	Studies on the application of temperature-responsive ion exchange polymers with whey proteins.
26905884	3	43	theme	dihydrogen	1096:1105	arg1	pH					1125:1126	pH 6.5	1125:1130	pH 6.5	1125:1130	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	43	theme	dihydrogen	1096:1105	arg1	buffer					1117:1122	the 10mM sodium dihydrogen phosphate buffer	1080:1122	the 10mM sodium dihydrogen phosphate buffer	1080:1122	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	4	44	theme	different	1188:1196	arg1	resins					1211:1216	these different ion exchange resins	1182:1216	these different ion exchange resins with bovine lactoferrin	1182:1240	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	2	45	from	°C	775:776	arg1	resin					717:721	14 mg bovine lactoferrin/mL resin	689:721	14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C	689:776	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	5	46	theme	anionic	1776:1782	arg1	β-lactoglobulin					1800:1814	bovine β-lactoglobulin	1793:1814	bovine β-lactoglobulin	1793:1814	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	5	46	theme	anionic	1776:1782	arg1	protein					1784:1790	the anionic protein	1772:1790	the anionic protein	1772:1790	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	3	47	theme	10mM	1084:1087	arg1	pH					1125:1126	pH 6.5	1125:1130	pH 6.5	1125:1130	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	47	theme	10mM	1084:1087	arg1	buffer					1117:1122	the 10mM sodium dihydrogen phosphate buffer	1080:1122	the 10mM sodium dihydrogen phosphate buffer	1080:1122	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	48	theme	temperature-responsive	944:965	arg1	ion-exchanger					967:979	this new temperature-responsive ion-exchanger	935:979	this new temperature-responsive ion-exchanger	935:979	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	4	49	theme	exchange	1202:1209	arg1	resins					1211:1216	these different ion exchange resins	1182:1216	these different ion exchange resins with bovine lactoferrin	1182:1240	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	5	50	theme	cationic	1600:1607	arg1	lactoperoxidase					1625:1639	bovine lactoperoxidase	1618:1639	bovine lactoperoxidase	1618:1639	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	5	50	theme	cationic	1600:1607	arg1	protein					1609:1615	The cationic protein	1596:1615	The cationic protein	1596:1615	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	4	51	theme	°C	1534:1535	arg1	conditions					1517:1526	different temperature conditions	1495:1526	different temperature conditions of 20 °C and 50 °C	1495:1545	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	1	52	theme	N-phenylacrylamide	266:283	arg1	co-polymerisation					245:261	the co-polymerisation	241:261	the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose	241:362	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	3	53	theme	buffer	1117:1122	arg1	composition					1065:1075	the composition	1061:1075	the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate	1061:1148	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	5	54	dep	adsorbed	1651:1658	arg1	whereas					1764:1770	whereas	1764:1770	whereas	1764:1770	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	4	55	theme	other	1287:1291	arg1	resins					1293:1298	other resins	1287:1298	other resins	1287:1298	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	1	56	theme	N-iso-propylacrylamide	286:307	arg1	co-polymerisation					245:261	the co-polymerisation	241:261	the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose	241:362	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	0	57	with	polymers	66:73	arg1	proteins					85:92	whey proteins	80:92	whey proteins	80:92	Studies on the application of temperature-responsive ion exchange polymers with whey proteins.
26905884	1	58	theme	composition	180:190	arg1	resins					152:157	temperature-responsive ion exchange resins	116:157	temperature-responsive ion exchange resins of different polymer composition	116:190	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	2	59	theme	whey	650:653	arg1	lactoferrin					671:681	bovine lactoferrin	664:681	bovine lactoferrin	664:681	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	59	theme	whey	650:653	arg1	protein					655:661	the whey protein	646:661	the whey protein	646:661	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	3	60	from	lactoferrin	861:871	arg1	protein					892:898	a normalised mg protein	876:898	a normalised mg protein per mL resin basis	876:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	2	61	theme	lactoferrin/mL	748:761	arg1	resin					763:767	62 mg bovine lactoferrin/mL resin	735:767	62 mg bovine lactoferrin/mL resin	735:767	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	3	62	theme	rate	1145:1148	arg1	composition					1065:1075	the composition	1061:1075	the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate	1061:1148	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	2	63	from	°C	728:729	arg1	resin					717:721	14 mg bovine lactoferrin/mL resin	689:721	14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C	689:776	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	5	64	theme	conditions	1867:1876	arg1	regime					1845:1850	this regime	1840:1850	this regime of temperature conditions	1840:1876	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	4	65	dep	resins	1293:1298	arg1	based					1300:1304	based	1300:1304	based on N-isopropylacrylamide	1300:1329	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	4	65	dep	resins	1293:1298	arg1	contained					1335:1343	contained	1335:1343	contained N-tert-butylacrylamide rather than N-phenylacrylamide	1335:1397	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	1	66	from	co-polymerisation	245:261	arg1	products					227:234	the products	223:234	the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose	223:362	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	2	67	theme	highest	607:613	arg1	Bmax					636:639	Bmax	636:639	Bmax	636:639	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	67	theme	highest	607:613	arg1	capacity					626:633	the highest adsorption capacity	603:633	the highest adsorption capacity	603:633	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	1	68	theme	ion	139:141	arg1	resins					152:157	temperature-responsive ion exchange resins	116:157	temperature-responsive ion exchange resins of different polymer composition	116:190	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	2	69	theme	mg	738:739	arg1	resin					763:767	62 mg bovine lactoferrin/mL resin	735:767	62 mg bovine lactoferrin/mL resin	735:767	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	3	70	theme	loading	807:813	arg1	conditions					815:824	dynamic loading conditions	799:824	dynamic loading conditions at 40 °C	799:833	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	1	71	theme	resins	152:157	arg1	types					107:111	Several new types	95:111	Several new types of temperature-responsive ion exchange resins of different polymer composition	95:190	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	3	72	theme	temperature	1019:1029	arg1	shift					1031:1035	a single cycle temperature shift	1004:1035	a single cycle temperature shift to 4 °C	1004:1043	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	2	73	dep	N-phenylacrylamide	546:563	arg1	acid					586:589	acrylic acid	578:589	acrylic acid	578:589	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	1	74	theme	different	162:170	arg1	composition					180:190	different polymer composition	162:190	different polymer composition	162:190	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	3	75	theme	lactoferrin	861:871	arg1	%					838:838	94%	836:838	94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis	836:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	75	theme	lactoferrin	861:871	arg1	lactoferrin					861:871	the loaded bovine lactoferrin	843:871	the loaded bovine lactoferrin on a normalised mg protein per mL resin basis	843:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	76	theme	cycle	1013:1017	arg1	shift					1031:1035	a single cycle temperature shift	1004:1035	a single cycle temperature shift to 4 °C	1004:1043	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	77	theme	single	1006:1011	arg1	shift					1031:1035	a single cycle temperature shift	1004:1035	a single cycle temperature shift to 4 °C	1004:1043	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	78	theme	loaded	847:852	arg1	lactoferrin					861:871	the loaded bovine lactoferrin	843:871	the loaded bovine lactoferrin on a normalised mg protein per mL resin basis	843:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	2	79	theme	bovine	664:669	arg1	lactoferrin					671:681	bovine lactoferrin	664:681	bovine lactoferrin	664:681	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	79	theme	bovine	664:669	arg1	protein					655:661	the whey protein	646:661	the whey protein	646:661	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	3	80	theme	mg	889:890	arg1	protein					892:898	a normalised mg protein	876:898	a normalised mg protein per mL resin basis	876:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	2	81	theme	bovine	695:700	arg1	resin					717:721	14 mg bovine lactoferrin/mL resin	689:721	14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C	689:776	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	82	theme	batch	441:445	arg1	conditions					458:467	batch adsorption conditions	441:467	batch adsorption conditions	441:467	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	2	83	theme	14	689:690	arg1	mg					692:693	mg	692:693	mg	692:693	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	4	84	theme	different	1495:1503	arg1	conditions					1517:1526	different temperature conditions	1495:1526	different temperature conditions of 20 °C and 50 °C	1495:1545	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	1	85	theme	acid	321:324	arg1	derivatives					326:336	acrylic acid derivatives	313:336	acrylic acid derivatives	313:336	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	1	86	theme	new	103:105	arg1	types					107:111	Several new types	95:111	Several new types of temperature-responsive ion exchange resins of different polymer composition	95:190	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	3	87	theme	resin	907:911	arg1	basis					913:917	mL resin basis	904:917	mL resin basis	904:917	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	4	88	theme	bovine	1223:1228	arg1	lactoferrin					1230:1240	bovine lactoferrin	1223:1240	bovine lactoferrin	1223:1240	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	4	89	theme	release	1444:1450	arg1	properties					1452:1461	release properties	1444:1461	release properties	1444:1461	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	0	90	theme	temperature-responsive	30:51	arg1	polymers					66:73	temperature-responsive ion exchange polymers	30:73	temperature-responsive ion exchange polymers with whey proteins	30:92	Studies on the application of temperature-responsive ion exchange polymers with whey proteins.
26905884	2	91	theme	%	538:538	arg1	N-phenylacrylamide					546:563	5% (w/w) N-phenylacrylamide	537:563	5% (w/w) N-phenylacrylamide	537:563	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	3	92	from	°C	832:833	arg1	conditions					815:824	dynamic loading conditions	799:824	dynamic loading conditions at 40 °C	799:833	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	5	93	theme	temperature-responsive	1684:1705	arg1	resins					1707:1712	these temperature-responsive resins	1678:1712	these temperature-responsive resins	1678:1712	The cationic protein, bovine lactoperoxidase, was also adsorbed and desorbed with these temperature-responsive resins under similar conditions of changing temperature, whereas the anionic protein, bovine β-lactoglobulin, was not adsorbed under this regime of temperature conditions but instead eluted in the flow-through.
26905884	0	94	theme	exchange	57:64	arg1	polymers					66:73	temperature-responsive ion exchange polymers	30:73	temperature-responsive ion exchange polymers with whey proteins	30:92	Studies on the application of temperature-responsive ion exchange polymers with whey proteins.
26905884	3	95	theme	sodium	1089:1094	arg1	pH					1125:1126	pH 6.5	1125:1130	pH 6.5	1125:1130	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	3	95	theme	sodium	1089:1094	arg1	buffer					1117:1122	the 10mM sodium dihydrogen phosphate buffer	1080:1122	the 10mM sodium dihydrogen phosphate buffer	1080:1122	Under dynamic loading conditions at 40 °C, 94% of the loaded bovine lactoferrin on a normalised mg protein per mL resin basis was adsorbed by this new temperature-responsive ion-exchanger, and 76% was eluted by a single cycle temperature shift to 4 °C without varying the composition of the 10mM sodium dihydrogen phosphate buffer, pH 6.5, or the flow rate.
26905884	4	96	theme	ion	1198:1200	arg1	resins					1211:1216	these different ion exchange resins	1182:1216	these different ion exchange resins with bovine lactoferrin	1182:1240	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	2	97	from	resin	763:767	arg1	resin					717:721	14 mg bovine lactoferrin/mL resin	689:721	14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C	689:776	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	1	98	link	cross-linked	343:354	arg1	agarose					356:362	cross-linked agarose	343:362	cross-linked agarose	343:362	Several new types of temperature-responsive ion exchange resins of different polymer composition have been prepared by grafting the products from the co-polymerisation of N-phenylacrylamide, N-iso-propylacrylamide and acrylic acid derivatives onto cross-linked agarose.
26905884	2	99	theme	binding	381:387	arg1	isotherms					389:397	the binding isotherms	377:397	the binding isotherms for these different resins obtained under batch adsorption conditions	377:467	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
26905884	4	100	theme	resins	1211:1216	arg1	characteristics					1163:1177	The binding characteristics	1151:1177	The binding characteristics of these different ion exchange resins with bovine lactoferrin	1151:1240	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	4	101	theme	°C	1544:1545	arg1	conditions					1517:1526	different temperature conditions	1495:1526	different temperature conditions of 20 °C and 50 °C	1495:1545	The binding characteristics of these different ion exchange resins with bovine lactoferrin were also compared to results obtained using other resins based on N-isopropylacrylamide but contained N-tert-butylacrylamide rather than N-phenylacrylamide, where the corresponding dynamic capture and release properties for bovine lactoferrin required different temperature conditions of 20 °C and 50 °C, respectively for optimal desorption/adsorption.
26905884	2	102	theme	different	409:417	arg1	resins					419:424	these different resins	403:424	these different resins obtained under batch adsorption conditions	403:467	Analysis of the binding isotherms for these different resins obtained under batch adsorption conditions indicated that the resin based on N-iso-propylacrylamide containing 5% (w/w) N-phenylacrylamide and 5% (w/w) acrylic acid resulted in the highest adsorption capacity, Bmax, for the whey protein, bovine lactoferrin, e.g. 14 mg bovine lactoferrin/mL resin at 4 °C and 62 mg bovine lactoferrin/mL resin at 40 °C, respectively.
25839803	6	0	theme	significant	842:852	arg1	differences					854:864	no significant differences	839:864	no significant differences in the crystallinity and the recorded Iα fraction of cellulose produced between Hestrin-Schramm and the HWE medium	839:979	There were no significant differences in the crystallinity and the recorded Iα fraction of cellulose produced between Hestrin-Schramm and the HWE medium.
25839803	3	1	theme	wood	468:471	arg1	extracts					483:490	wood hot water extracts	468:490	wood hot water extracts	468:490	In this research, a relatively low-cost culture media was successfully developed from wood hot water extracts for the Acetobacter xylinus 23769 strain.
25839803	5	2	theme	different	727:735	arg1	pH					737:738	different pH	727:738	different pH (5, 6, 7 and 8)	727:754	The effects of different pH (5, 6, 7 and 8) and temperatures (26, 28 and 30°C) were also examined in this research.
25839803	8	3	theme	lignocellulosic	1344:1358	arg1	feedstocks					1360:1369	lignocellulosic feedstocks	1344:1369	lignocellulosic feedstocks	1344:1369	HWE was shown to be a suitable carbon source for BC production, and a process was established for BC production from lignocellulosic feedstocks without using any modification of the HWE.
25839803	7	4	theme	BC	1019:1020	arg1	0.15g/l					1008:1014	0.15g/l	1008:1014	0.15g/l of BC	1008:1020	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	7	5	from	HWE	1099:1101	arg1	temperature					1052:1062	temperature	1052:1062	temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results	1052:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	7	5	from	HWE	1099:1101	arg1	pH					1040:1041	a pH	1038:1041	a pH of 8	1038:1046	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	1	6	theme	Acetobacter	132:142	arg1	xylinum					144:150	Acetobacter xylinum	132:150	Acetobacter xylinum	132:150	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	4	7	theme	water	538:542	arg1	material					572:579	a residual material	561:579	a residual material originating from pulp mills and lignocellulosic biorefineries	561:641	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	4	7	theme	water	538:542	arg1	HWE					553:555	HWE	553:555	HWE	553:555	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	4	7	theme	water	538:542	arg1	extract					544:550	Hot water extract	534:550	Hot water extract (HWE)	534:556	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	7	8	theme	28°C.	1067:1071	arg1	Glucose					1073:1079	28°C. Glucose	1067:1079	28°C. Glucose	1067:1079	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	5	9	dep	temperatures	760:771	arg1	30°C					785:788	30°C	785:788	30°C	785:788	The effects of different pH (5, 6, 7 and 8) and temperatures (26, 28 and 30°C) were also examined in this research.
25839803	5	9	dep	temperatures	760:771	arg1	28					778:779	28	778:779	28	778:779	The effects of different pH (5, 6, 7 and 8) and temperatures (26, 28 and 30°C) were also examined in this research.
25839803	8	10	theme	suitable	1249:1256	arg1	source					1265:1270	a suitable carbon source	1247:1270	a suitable carbon source for BC production	1247:1288	HWE was shown to be a suitable carbon source for BC production, and a process was established for BC production from lignocellulosic feedstocks without using any modification of the HWE.
25839803	8	10	theme	suitable	1249:1256	arg1	HWE					1227:1229	HWE	1227:1229	HWE	1227:1229	HWE was shown to be a suitable carbon source for BC production, and a process was established for BC production from lignocellulosic feedstocks without using any modification of the HWE.
25839803	3	11	theme	Acetobacter	500:510	arg1	strain					526:531	the Acetobacter xylinus 23769 strain	496:531	the Acetobacter xylinus 23769 strain	496:531	In this research, a relatively low-cost culture media was successfully developed from wood hot water extracts for the Acetobacter xylinus 23769 strain.
25839803	10	12	theme	production	1592:1601	arg1	goal					1569:1572	the goal	1565:1572	the goal of large scale BC production	1565:1601	Using HWE for BC production could reduce burdens on the environment and also, achieve the goal of large scale BC production at low cost without using added culture nutrients.
25839803	7	13	theme	Glucose	1073:1079	arg1	temperature					1052:1062	temperature	1052:1062	temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results	1052:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	7	13	theme	Glucose	1073:1079	arg1	pH					1040:1041	a pH	1038:1041	a pH of 8	1038:1046	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	7	14	from	pH	1040:1041	arg1	HWE					1099:1101	the HWE	1095:1101	the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results	1095:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	7	15	theme	0.15g/l	1008:1014	arg1	production					994:1003	The maximum production	982:1003	The maximum production of 0.15g/l of BC	982:1020	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	3	16	theme	xylinus	512:518	arg1	strain					526:531	the Acetobacter xylinus 23769 strain	496:531	the Acetobacter xylinus 23769 strain	496:531	In this research, a relatively low-cost culture media was successfully developed from wood hot water extracts for the Acetobacter xylinus 23769 strain.
25839803	4	17	theme	pulp	598:601	arg1	mills					603:607	pulp mills	598:607	pulp mills	598:607	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	7	18	theme	maximum	986:992	arg1	production					994:1003	The maximum production	982:1003	The maximum production of 0.15g/l of BC	982:1020	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	10	19	theme	large	1577:1581	arg1	production					1592:1601	large scale BC production	1577:1601	large scale BC production	1577:1601	Using HWE for BC production could reduce burdens on the environment and also, achieve the goal of large scale BC production at low cost without using added culture nutrients.
25839803	10	20	theme	BC	1493:1494	arg1	production					1496:1505	BC production	1493:1505	BC production	1493:1505	Using HWE for BC production could reduce burdens on the environment and also, achieve the goal of large scale BC production at low cost without using added culture nutrients.
25839803	7	21	theme	liquid	1189:1194	arg1	HPLC					1212:1215	HPLC	1212:1215	HPLC	1212:1215	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	7	21	theme	liquid	1189:1194	arg1	chromatography					1196:1209	high-pressure liquid chromatography	1175:1209	high-pressure liquid chromatography (HPLC) results	1175:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	8	22	from	feedstocks	1360:1369	arg1	production					1328:1337	BC production	1325:1337	BC production from lignocellulosic feedstocks	1325:1369	HWE was shown to be a suitable carbon source for BC production, and a process was established for BC production from lignocellulosic feedstocks without using any modification of the HWE.
25839803	10	23	theme	scale	1583:1587	arg1	production					1592:1601	large scale BC production	1577:1601	large scale BC production	1577:1601	Using HWE for BC production could reduce burdens on the environment and also, achieve the goal of large scale BC production at low cost without using added culture nutrients.
25839803	5	24	theme	pH	737:738	arg1	effects					716:722	The effects	712:722	The effects of different pH (5, 6, 7 and 8) and temperatures (26, 28 and 30°C)	712:789	The effects of different pH (5, 6, 7 and 8) and temperatures (26, 28 and 30°C) were also examined in this research.
25839803	1	25	theme	Bacterial	72:80	arg1	type					100:103	a type	98:103	a type of nanopolymer produced by Acetobacter xylinum	98:150	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	1	25	theme	Bacterial	72:80	arg1	BC					93:94	BC	93:94	BC	93:94	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	1	25	theme	Bacterial	72:80	arg1	material					172:179	a nanostructured material	155:179	a nanostructured material with unique properties and wide applicability	155:225	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	1	25	theme	Bacterial	72:80	arg1	cellulose					82:90	Bacterial cellulose	72:90	Bacterial cellulose (BC)	72:95	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	6	26	theme	HWE	970:972	arg1	medium					974:979	the HWE medium	966:979	the HWE medium	966:979	There were no significant differences in the crystallinity and the recorded Iα fraction of cellulose produced between Hestrin-Schramm and the HWE medium.
25839803	7	27	theme	8	1046:1046	arg1	temperature					1052:1062	temperature	1052:1062	temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results	1052:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	7	27	theme	8	1046:1046	arg1	pH					1040:1041	a pH	1038:1041	a pH of 8	1038:1046	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	7	28	theme	xylose	1085:1090	arg1	temperature					1052:1062	temperature	1052:1062	temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results	1052:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	7	28	theme	xylose	1085:1090	arg1	pH					1040:1041	a pH	1038:1041	a pH of 8	1038:1046	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	5	29	dep	pH	737:738	arg1	8					753:753	8	753:753	8	753:753	The effects of different pH (5, 6, 7 and 8) and temperatures (26, 28 and 30°C) were also examined in this research.
25839803	5	29	dep	pH	737:738	arg1	7					747:747	7	747:747	7	747:747	The effects of different pH (5, 6, 7 and 8) and temperatures (26, 28 and 30°C) were also examined in this research.
25839803	10	30	theme	low	1606:1608	arg1	cost					1610:1613	low cost	1606:1613	low cost	1606:1613	Using HWE for BC production could reduce burdens on the environment and also, achieve the goal of large scale BC production at low cost without using added culture nutrients.
25839803	2	31	theme	scale	346:350	arg1	extension					352:360	wide scale extension	341:360	wide scale extension of BC applications	341:379	However, a standard medium used for the cultivation of BC, the Hestrin-Schramm medium, is expensive and prevents wide scale extension of BC applications.
25839803	2	32	theme	BC	283:284	arg1	cultivation					268:278	the cultivation	264:278	the cultivation of BC, the Hestrin-Schramm medium,	264:313	However, a standard medium used for the cultivation of BC, the Hestrin-Schramm medium, is expensive and prevents wide scale extension of BC applications.
25839803	7	33	theme	BC	1150:1151	arg1	cultivations					1153:1164	all BC cultivations	1146:1164	all BC cultivations based on high-pressure liquid chromatography (HPLC) results	1146:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	1	34	theme	nanostructured	157:170	arg1	cellulose					82:90	Bacterial cellulose	72:90	Bacterial cellulose (BC)	72:95	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	1	34	theme	nanostructured	157:170	arg1	material					172:179	a nanostructured material	155:179	a nanostructured material with unique properties and wide applicability	155:225	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	0	35	theme	cellulose	23:31	arg1	water					43:47	bacterial cellulose using hot water	13:47	bacterial cellulose using hot water	13:47	Synthesis of bacterial cellulose using hot water extracted wood sugars.
25839803	10	36	theme	BC	1589:1590	arg1	production					1592:1601	large scale BC production	1577:1601	large scale BC production	1577:1601	Using HWE for BC production could reduce burdens on the environment and also, achieve the goal of large scale BC production at low cost without using added culture nutrients.
25839803	3	37	theme	23769	520:524	arg1	strain					526:531	the Acetobacter xylinus 23769 strain	496:531	the Acetobacter xylinus 23769 strain	496:531	In this research, a relatively low-cost culture media was successfully developed from wood hot water extracts for the Acetobacter xylinus 23769 strain.
25839803	10	38	theme	culture	1635:1641	arg1	nutrients					1643:1651	added culture nutrients	1629:1651	added culture nutrients	1629:1651	Using HWE for BC production could reduce burdens on the environment and also, achieve the goal of large scale BC production at low cost without using added culture nutrients.
25839803	3	39	theme	culture	422:428	arg1	media					430:434	a relatively low-cost culture media	400:434	a relatively low-cost culture media	400:434	In this research, a relatively low-cost culture media was successfully developed from wood hot water extracts for the Acetobacter xylinus 23769 strain.
25839803	0	40	theme	bacterial	13:21	arg1	water					43:47	bacterial cellulose using hot water	13:47	bacterial cellulose using hot water	13:47	Synthesis of bacterial cellulose using hot water extracted wood sugars.
25839803	4	41	theme	Hot	534:536	arg1	material					572:579	a residual material	561:579	a residual material originating from pulp mills and lignocellulosic biorefineries	561:641	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	4	41	theme	Hot	534:536	arg1	HWE					553:555	HWE	553:555	HWE	553:555	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	4	41	theme	Hot	534:536	arg1	extract					544:550	Hot water extract	534:550	Hot water extract (HWE)	534:556	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	7	42	from	temperature	1052:1062	arg1	HWE					1099:1101	the HWE	1095:1101	the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results	1095:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	3	43	theme	hot	473:475	arg1	extracts					483:490	wood hot water extracts	468:490	wood hot water extracts	468:490	In this research, a relatively low-cost culture media was successfully developed from wood hot water extracts for the Acetobacter xylinus 23769 strain.
25839803	8	44	theme	HWE	1409:1411	arg1	modification					1389:1400	any modification	1385:1400	any modification of the HWE	1385:1411	HWE was shown to be a suitable carbon source for BC production, and a process was established for BC production from lignocellulosic feedstocks without using any modification of the HWE.
25839803	3	45	theme	water	477:481	arg1	extracts					483:490	wood hot water extracts	468:490	wood hot water extracts	468:490	In this research, a relatively low-cost culture media was successfully developed from wood hot water extracts for the Acetobacter xylinus 23769 strain.
25839803	0	46	theme	hot	39:41	arg1	water					43:47	bacterial cellulose using hot water	13:47	bacterial cellulose using hot water	13:47	Synthesis of bacterial cellulose using hot water extracted wood sugars.
25839803	4	47	theme	monomeric	666:674	arg1	sugars					676:681	mainly monomeric sugars	659:681	mainly monomeric sugars	659:681	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	1	48	with	material	172:179	arg1	applicability					213:225	wide applicability	208:225	wide applicability	208:225	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	1	48	with	material	172:179	arg1	properties					193:202	unique properties	186:202	unique properties	186:202	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	1	49	theme	unique	186:191	arg1	properties					193:202	unique properties	186:202	unique properties	186:202	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	9	50	theme	abundant	1424:1431	arg1	by-product					1467:1476	an abundant and relatively inexpensive forest by-product	1421:1476	an abundant and relatively inexpensive forest by-product	1421:1476	HWE is an abundant and relatively inexpensive forest by-product.
25839803	9	50	theme	abundant	1424:1431	arg1	HWE					1414:1416	HWE	1414:1416	HWE	1414:1416	HWE is an abundant and relatively inexpensive forest by-product.
25839803	2	51	theme	wide	341:344	arg1	extension					352:360	wide scale extension	341:360	wide scale extension of BC applications	341:379	However, a standard medium used for the cultivation of BC, the Hestrin-Schramm medium, is expensive and prevents wide scale extension of BC applications.
25839803	2	52	theme	applications	368:379	arg1	extension					352:360	wide scale extension	341:360	wide scale extension of BC applications	341:379	However, a standard medium used for the cultivation of BC, the Hestrin-Schramm medium, is expensive and prevents wide scale extension of BC applications.
25839803	0	53	theme	using	33:37	arg1	water					43:47	bacterial cellulose using hot water	13:47	bacterial cellulose using hot water	13:47	Synthesis of bacterial cellulose using hot water extracted wood sugars.
25839803	6	54	from	differences	854:864	arg1	fraction					907:914	the recorded Iα fraction	891:914	the recorded Iα fraction	891:914	There were no significant differences in the crystallinity and the recorded Iα fraction of cellulose produced between Hestrin-Schramm and the HWE medium.
25839803	6	54	from	differences	854:864	arg1	crystallinity					873:885	crystallinity	873:885	crystallinity	873:885	There were no significant differences in the crystallinity and the recorded Iα fraction of cellulose produced between Hestrin-Schramm and the HWE medium.
25839803	6	55	theme	Iα	904:905	arg1	fraction					907:914	the recorded Iα fraction	891:914	the recorded Iα fraction	891:914	There were no significant differences in the crystallinity and the recorded Iα fraction of cellulose produced between Hestrin-Schramm and the HWE medium.
25839803	5	56	theme	temperatures	760:771	arg1	effects					716:722	The effects	712:722	The effects of different pH (5, 6, 7 and 8) and temperatures (26, 28 and 30°C)	712:789	The effects of different pH (5, 6, 7 and 8) and temperatures (26, 28 and 30°C) were also examined in this research.
25839803	2	57	theme	BC	365:366	arg1	applications					368:379	BC applications	365:379	BC applications	365:379	However, a standard medium used for the cultivation of BC, the Hestrin-Schramm medium, is expensive and prevents wide scale extension of BC applications.
25839803	6	58	theme	cellulose	919:927	arg1	fraction					907:914	the recorded Iα fraction	891:914	the recorded Iα fraction	891:914	There were no significant differences in the crystallinity and the recorded Iα fraction of cellulose produced between Hestrin-Schramm and the HWE medium.
25839803	6	58	theme	cellulose	919:927	arg1	crystallinity					873:885	crystallinity	873:885	crystallinity	873:885	There were no significant differences in the crystallinity and the recorded Iα fraction of cellulose produced between Hestrin-Schramm and the HWE medium.
25839803	7	59	from	Glucose	1073:1079	arg1	HWE					1099:1101	the HWE	1095:1101	the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results	1095:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	8	60	theme	BC	1325:1326	arg1	production					1328:1337	BC production	1325:1337	BC production from lignocellulosic feedstocks	1325:1369	HWE was shown to be a suitable carbon source for BC production, and a process was established for BC production from lignocellulosic feedstocks without using any modification of the HWE.
25839803	0	61	theme	water	43:47	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of bacterial cellulose using hot water	0:47	Synthesis of bacterial cellulose using hot water extracted wood sugars.
25839803	7	62	theme	high-pressure	1175:1187	arg1	HPLC					1212:1215	HPLC	1212:1215	HPLC	1212:1215	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	7	62	theme	high-pressure	1175:1187	arg1	chromatography					1196:1209	high-pressure liquid chromatography	1175:1209	high-pressure liquid chromatography (HPLC) results	1175:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	9	63	theme	inexpensive	1448:1458	arg1	by-product					1467:1476	an abundant and relatively inexpensive forest by-product	1421:1476	an abundant and relatively inexpensive forest by-product	1421:1476	HWE is an abundant and relatively inexpensive forest by-product.
25839803	9	63	theme	inexpensive	1448:1458	arg1	HWE					1414:1416	HWE	1414:1416	HWE	1414:1416	HWE is an abundant and relatively inexpensive forest by-product.
25839803	4	64	theme	lignocellulosic	613:627	arg1	biorefineries					629:641	lignocellulosic biorefineries	613:641	lignocellulosic biorefineries	613:641	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	10	65	dep	reduce	1513:1518	arg1	achieve					1557:1563	achieve	1557:1563	achieve the goal of large scale BC production at low cost without using added culture nutrients	1557:1651	Using HWE for BC production could reduce burdens on the environment and also, achieve the goal of large scale BC production at low cost without using added culture nutrients.
25839803	7	66	from	xylose	1085:1090	arg1	HWE					1099:1101	the HWE	1095:1101	the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results	1095:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	2	67	theme	standard	239:246	arg1	expensive					318:326	expensive	318:326	expensive	318:326	However, a standard medium used for the cultivation of BC, the Hestrin-Schramm medium, is expensive and prevents wide scale extension of BC applications.
25839803	2	67	theme	standard	239:246	arg1	medium					248:253	a standard medium	237:253	a standard medium used for the cultivation of BC, the Hestrin-Schramm medium,	237:313	However, a standard medium used for the cultivation of BC, the Hestrin-Schramm medium, is expensive and prevents wide scale extension of BC applications.
25839803	10	68	theme	added	1629:1633	arg1	nutrients					1643:1651	added culture nutrients	1629:1651	added culture nutrients	1629:1651	Using HWE for BC production could reduce burdens on the environment and also, achieve the goal of large scale BC production at low cost without using added culture nutrients.
25839803	2	69	theme	Hestrin-Schramm	291:305	arg1	medium					307:312	the Hestrin-Schramm medium	287:312	the Hestrin-Schramm medium	287:312	However, a standard medium used for the cultivation of BC, the Hestrin-Schramm medium, is expensive and prevents wide scale extension of BC applications.
25839803	2	69	theme	Hestrin-Schramm	291:305	arg1	BC					283:284	BC	283:284	BC	283:284	However, a standard medium used for the cultivation of BC, the Hestrin-Schramm medium, is expensive and prevents wide scale extension of BC applications.
25839803	4	70	theme	organic	684:690	arg1	acids					692:696	organic acids	684:696	organic acids	684:696	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	1	71	theme	wide	208:211	arg1	applicability					213:225	wide applicability	208:225	wide applicability	208:225	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	8	72	theme	carbon	1258:1263	arg1	source					1265:1270	a suitable carbon source	1247:1270	a suitable carbon source for BC production	1247:1288	HWE was shown to be a suitable carbon source for BC production, and a process was established for BC production from lignocellulosic feedstocks without using any modification of the HWE.
25839803	8	72	theme	carbon	1258:1263	arg1	HWE					1227:1229	HWE	1227:1229	HWE	1227:1229	HWE was shown to be a suitable carbon source for BC production, and a process was established for BC production from lignocellulosic feedstocks without using any modification of the HWE.
25839803	3	73	theme	low-cost	413:420	arg1	media					430:434	a relatively low-cost culture media	400:434	a relatively low-cost culture media	400:434	In this research, a relatively low-cost culture media was successfully developed from wood hot water extracts for the Acetobacter xylinus 23769 strain.
25839803	8	74	theme	BC	1276:1277	arg1	production					1279:1288	BC production	1276:1288	BC production	1276:1288	HWE was shown to be a suitable carbon source for BC production, and a process was established for BC production from lignocellulosic feedstocks without using any modification of the HWE.
25839803	9	75	theme	forest	1460:1465	arg1	by-product					1467:1476	an abundant and relatively inexpensive forest by-product	1421:1476	an abundant and relatively inexpensive forest by-product	1421:1476	HWE is an abundant and relatively inexpensive forest by-product.
25839803	9	75	theme	forest	1460:1465	arg1	HWE					1414:1416	HWE	1414:1416	HWE	1414:1416	HWE is an abundant and relatively inexpensive forest by-product.
25839803	1	76	theme	nanopolymer	108:118	arg1	type					100:103	a type	98:103	a type of nanopolymer produced by Acetobacter xylinum	98:150	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	1	76	theme	nanopolymer	108:118	arg1	cellulose					82:90	Bacterial cellulose	72:90	Bacterial cellulose (BC)	72:95	Bacterial cellulose (BC), a type of nanopolymer produced by Acetobacter xylinum is a nanostructured material with unique properties and wide applicability.
25839803	7	77	theme	chromatography	1196:1209	arg1	results					1218:1224	high-pressure liquid chromatography (HPLC) results	1175:1224	high-pressure liquid chromatography (HPLC) results	1175:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	4	78	theme	residual	563:570	arg1	material					572:579	a residual material	561:579	a residual material originating from pulp mills and lignocellulosic biorefineries	561:641	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	4	78	theme	residual	563:570	arg1	extract					544:550	Hot water extract	534:550	Hot water extract (HWE)	534:556	Hot water extract (HWE) is a residual material originating from pulp mills and lignocellulosic biorefineries and consists of mainly monomeric sugars, organic acids and organics.
25839803	0	79	theme	wood	59:62	arg1	sugars					64:69	wood sugars	59:69	wood sugars	59:69	Synthesis of bacterial cellulose using hot water extracted wood sugars.
25839803	6	80	dep	crystallinity	873:885	arg1	the					869:871	the	869:871	the	869:871	There were no significant differences in the crystallinity and the recorded Iα fraction of cellulose produced between Hestrin-Schramm and the HWE medium.
25839803	7	81	theme	nutrient	1117:1124	arg1	sources					1126:1132	the main nutrient sources	1108:1132	the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results	1108:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	7	82	theme	main	1112:1115	arg1	sources					1126:1132	the main nutrient sources	1108:1132	the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results	1108:1224	The maximum production of 0.15g/l of BC was obtained at a pH of 8 and temperature of 28°C. Glucose and xylose in the HWE were the main nutrient sources utilized in all BC cultivations based on high-pressure liquid chromatography (HPLC) results.
25839803	6	83	theme	recorded	895:902	arg1	fraction					907:914	the recorded Iα fraction	891:914	the recorded Iα fraction	891:914	There were no significant differences in the crystallinity and the recorded Iα fraction of cellulose produced between Hestrin-Schramm and the HWE medium.
28332587	0	0	theme	novel	93:97	arg1	hydrogel					139:146	a biocompatible novel thermo-reversible methylcellulose-based hydrogel	77:146	a biocompatible novel thermo-reversible methylcellulose-based hydrogel	77:146	Bioreactor mechanically guided 3D mesenchymal stem cell chondrogenesis using a biocompatible novel thermo-reversible methylcellulose-based hydrogel.
28332587	5	1	theme	mechanical	809:818	arg1	properties					820:829	mechanical properties	809:829	mechanical properties	809:829	MC hydrogel was obtained by dispersion technique and its thermo-reversibility, mechanical properties, degradation and swelling were investigated, demonstrating a solution-gelation transition between 34 and 37 °C and a low bulk degradation (<20%) after 1 month.
28332587	5	2	theme	bulk	952:955	arg1	degradation					957:967	a low bulk degradation	946:967	a low bulk degradation (<20%)	946:974	MC hydrogel was obtained by dispersion technique and its thermo-reversibility, mechanical properties, degradation and swelling were investigated, demonstrating a solution-gelation transition between 34 and 37 °C and a low bulk degradation (<20%) after 1 month.
28332587	5	2	theme	bulk	952:955	arg1	%					973:973	<20%	970:973	<20%	970:973	MC hydrogel was obtained by dispersion technique and its thermo-reversibility, mechanical properties, degradation and swelling were investigated, demonstrating a solution-gelation transition between 34 and 37 °C and a low bulk degradation (<20%) after 1 month.
28332587	4	3	theme	novel	628:632	arg1	hydrogel					652:659	a novel thermo-reversible hydrogel	626:659	a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution	626:727	Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution.
28332587	0	4	theme	biocompatible	79:91	arg1	hydrogel					139:146	a biocompatible novel thermo-reversible methylcellulose-based hydrogel	77:146	a biocompatible novel thermo-reversible methylcellulose-based hydrogel	77:146	Bioreactor mechanically guided 3D mesenchymal stem cell chondrogenesis using a biocompatible novel thermo-reversible methylcellulose-based hydrogel.
28332587	9	5	theme	induced	1586:1592	arg1	chondrogenesis					1599:1612	load induced MSCs chondrogenesis	1581:1612	load induced MSCs chondrogenesis	1581:1612	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	0	6	theme	methylcellulose-based	117:137	arg1	hydrogel					139:146	a biocompatible novel thermo-reversible methylcellulose-based hydrogel	77:146	a biocompatible novel thermo-reversible methylcellulose-based hydrogel	77:146	Bioreactor mechanically guided 3D mesenchymal stem cell chondrogenesis using a biocompatible novel thermo-reversible methylcellulose-based hydrogel.
28332587	9	7	from	increase	1385:1392	arg1	expression					1415:1424	chondrogenic gene expression	1397:1424	chondrogenic gene expression	1397:1424	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	5	8	theme	low	948:950	arg1	degradation					957:967	a low bulk degradation	946:967	a low bulk degradation (<20%)	946:974	MC hydrogel was obtained by dispersion technique and its thermo-reversibility, mechanical properties, degradation and swelling were investigated, demonstrating a solution-gelation transition between 34 and 37 °C and a low bulk degradation (<20%) after 1 month.
28332587	5	8	theme	low	948:950	arg1	%					973:973	<20%	970:973	<20%	970:973	MC hydrogel was obtained by dispersion technique and its thermo-reversibility, mechanical properties, degradation and swelling were investigated, demonstrating a solution-gelation transition between 34 and 37 °C and a low bulk degradation (<20%) after 1 month.
28332587	6	9	theme	immunoreaction	1024:1037	arg1	lack					995:998	The lack	991:998	The lack of any hydrogel-derived immunoreaction	991:1037	The lack of any hydrogel-derived immunoreaction was demonstrated in vivo by mice subcutaneous implantation.
28332587	0	10	theme	thermo-reversible	99:115	arg1	hydrogel					139:146	a biocompatible novel thermo-reversible methylcellulose-based hydrogel	77:146	a biocompatible novel thermo-reversible methylcellulose-based hydrogel	77:146	Bioreactor mechanically guided 3D mesenchymal stem cell chondrogenesis using a biocompatible novel thermo-reversible methylcellulose-based hydrogel.
28332587	7	11	theme	MC	1156:1157	arg1	solution					1159:1166	MC solution	1156:1166	MC solution retained within a porous polyurethane (PU) matrix	1156:1216	To induce in vitro chondrogenesis, MSCs were seeded into MC solution retained within a porous polyurethane (PU) matrix.
28332587	7	12	theme	porous	1186:1191	arg1	matrix					1211:1216	a porous polyurethane (PU) matrix	1184:1216	a porous polyurethane (PU) matrix	1184:1216	To induce in vitro chondrogenesis, MSCs were seeded into MC solution retained within a porous polyurethane (PU) matrix.
28332587	4	13	theme	thermo-reversible	634:650	arg1	hydrogel					652:659	a novel thermo-reversible hydrogel	626:659	a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution	626:727	Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution.
28332587	5	14	theme	solution-gelation	892:908	arg1	transition					910:919	a solution-gelation transition	890:919	a solution-gelation transition between 34 and 37 °C	890:940	MC hydrogel was obtained by dispersion technique and its thermo-reversibility, mechanical properties, degradation and swelling were investigated, demonstrating a solution-gelation transition between 34 and 37 °C and a low bulk degradation (<20%) after 1 month.
28332587	4	15	theme	w/v	676:678	arg1	MC					697:698	MC	697:698	MC	697:698	Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution.
28332587	4	15	theme	w/v	676:678	arg1	methylcellulose					680:694	8% w/v methylcellulose	673:694	8% w/v methylcellulose (MC)	673:699	Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution.
28332587	6	16	theme	mice	1067:1070	arg1	implantation					1085:1096	mice subcutaneous implantation	1067:1096	mice subcutaneous implantation	1067:1096	The lack of any hydrogel-derived immunoreaction was demonstrated in vivo by mice subcutaneous implantation.
28332587	8	17	theme	shear	1287:1291	arg1	forces					1293:1298	shear forces	1287:1298	shear forces	1287:1298	PU-MC composites were subjected to a combination of compression and shear forces for 21 days in a custom made bioreactor.
28332587	8	18	theme	compression	1271:1281	arg1	combination					1256:1266	a combination	1254:1266	a combination of compression and shear forces	1254:1298	PU-MC composites were subjected to a combination of compression and shear forces for 21 days in a custom made bioreactor.
28332587	6	19	theme	hydrogel-derived	1007:1022	arg1	immunoreaction					1024:1037	any hydrogel-derived immunoreaction	1003:1037	any hydrogel-derived immunoreaction	1003:1037	The lack of any hydrogel-derived immunoreaction was demonstrated in vivo by mice subcutaneous implantation.
28332587	9	20	theme	Mechanical	1341:1350	arg1	stimulation					1352:1362	Mechanical stimulation	1341:1362	Mechanical stimulation	1341:1362	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	3	21	with	interaction	586:596	arg1	cells					603:607	cells	603:607	cells	603:607	Moreover, MSC supporting material selection is fundamental to allow for an active interaction with cells.
28332587	8	22	theme	PU-MC	1219:1223	arg1	composites					1225:1234	PU-MC composites	1219:1234	PU-MC composites	1219:1234	PU-MC composites were subjected to a combination of compression and shear forces for 21 days in a custom made bioreactor.
28332587	9	23	theme	significant	1373:1383	arg1	increase					1385:1392	a significant increase	1371:1392	a significant increase in chondrogenic gene expression	1371:1424	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	4	24	theme	0.05 M	706:711	arg1	solution					720:727	a 0.05 M Na2SO4 solution	704:727	a 0.05 M Na2SO4 solution	704:727	Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution.
28332587	6	25	link	hydrogel-derived	1007:1022	arg1	immunoreaction					1024:1037	any hydrogel-derived immunoreaction	1003:1037	any hydrogel-derived immunoreaction	1003:1037	The lack of any hydrogel-derived immunoreaction was demonstrated in vivo by mice subcutaneous implantation.
28332587	8	26	theme	forces	1293:1298	arg1	combination					1256:1266	a combination	1254:1266	a combination of compression and shear forces	1254:1298	PU-MC composites were subjected to a combination of compression and shear forces for 21 days in a custom made bioreactor.
28332587	9	27	theme	histological	1433:1444	arg1	analysis					1446:1453	histological analysis	1433:1453	histological analysis	1433:1453	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	2	28	theme	factors	495:501	arg1	use					478:480	the use	474:480	the use of exogenous factors	474:501	Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors.
28332587	9	29	theme	loaded	1517:1522	arg1	specimens					1524:1532	loaded specimens	1517:1532	loaded specimens	1517:1532	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	2	30	theme	exogenous	485:493	arg1	factors					495:501	exogenous factors	485:501	exogenous factors	485:501	Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors.
28332587	9	31	theme	hydrogel	1549:1556	arg1	suitability					1558:1568	MC hydrogel suitability	1546:1568	MC hydrogel suitability	1546:1568	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	3	32	theme	material	529:536	arg1	selection					538:546	material selection	529:546	material selection	529:546	Moreover, MSC supporting material selection is fundamental to allow for an active interaction with cells.
28332587	0	33	theme	stem	46:49	arg1	cell					51:54	3D mesenchymal stem cell	31:54	3D mesenchymal stem cell chondrogenesis	31:69	Bioreactor mechanically guided 3D mesenchymal stem cell chondrogenesis using a biocompatible novel thermo-reversible methylcellulose-based hydrogel.
28332587	9	34	theme	MC	1546:1547	arg1	suitability					1558:1568	MC hydrogel suitability	1546:1568	MC hydrogel suitability	1546:1568	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	0	35	theme	mesenchymal	34:44	arg1	cell					51:54	3D mesenchymal stem cell	31:54	3D mesenchymal stem cell chondrogenesis	31:69	Bioreactor mechanically guided 3D mesenchymal stem cell chondrogenesis using a biocompatible novel thermo-reversible methylcellulose-based hydrogel.
28332587	7	36	theme	polyurethane	1193:1204	arg1	matrix					1211:1216	a porous polyurethane (PU) matrix	1184:1216	a porous polyurethane (PU) matrix	1184:1216	To induce in vitro chondrogenesis, MSCs were seeded into MC solution retained within a porous polyurethane (PU) matrix.
28332587	0	37	theme	3D	31:32	arg1	cell					51:54	3D mesenchymal stem cell	31:54	3D mesenchymal stem cell chondrogenesis	31:69	Bioreactor mechanically guided 3D mesenchymal stem cell chondrogenesis using a biocompatible novel thermo-reversible methylcellulose-based hydrogel.
28332587	1	38	theme	poor	271:274	arg1	capacity					286:293	chondrocytes' poor expansion capacity	257:293	chondrocytes' poor expansion capacity	257:293	Autologous chondrocyte implantation for cartilage repair represents a challenge because strongly limited by chondrocytes' poor expansion capacity in vitro.
28332587	5	39	theme	dispersion	758:767	arg1	technique					769:777	dispersion technique	758:777	dispersion technique	758:777	MC hydrogel was obtained by dispersion technique and its thermo-reversibility, mechanical properties, degradation and swelling were investigated, demonstrating a solution-gelation transition between 34 and 37 °C and a low bulk degradation (<20%) after 1 month.
28332587	1	40	theme	Autologous	149:158	arg1	implantation					172:183	Autologous chondrocyte implantation	149:183	Autologous chondrocyte implantation for cartilage repair	149:204	Autologous chondrocyte implantation for cartilage repair represents a challenge because strongly limited by chondrocytes' poor expansion capacity in vitro.
28332587	0	41	theme	cell	51:54	arg1	chondrogenesis					56:69	3D mesenchymal stem cell chondrogenesis	31:69	3D mesenchymal stem cell chondrogenesis	31:69	Bioreactor mechanically guided 3D mesenchymal stem cell chondrogenesis using a biocompatible novel thermo-reversible methylcellulose-based hydrogel.
28332587	9	42	theme	chondrogenic	1397:1408	arg1	expression					1415:1424	chondrogenic gene expression	1397:1424	chondrogenic gene expression	1397:1424	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	9	43	theme	MSCs	1594:1597	arg1	chondrogenesis					1599:1612	load induced MSCs chondrogenesis	1581:1612	load induced MSCs chondrogenesis	1581:1612	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	1	44	theme	chondrocyte	160:170	arg1	implantation					172:183	Autologous chondrocyte implantation	149:183	Autologous chondrocyte implantation for cartilage repair	149:204	Autologous chondrocyte implantation for cartilage repair represents a challenge because strongly limited by chondrocytes' poor expansion capacity in vitro.
28332587	9	45	theme	gene	1410:1413	arg1	expression					1415:1424	chondrogenic gene expression	1397:1424	chondrogenic gene expression	1397:1424	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	1	46	theme	cartilage	189:197	arg1	repair					199:204	cartilage repair	189:204	cartilage repair	189:204	Autologous chondrocyte implantation for cartilage repair represents a challenge because strongly limited by chondrocytes' poor expansion capacity in vitro.
28332587	2	47	theme	mechanical	378:387	arg1	loading					389:395	mechanical loading	378:395	mechanical loading	378:395	Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors.
28332587	2	47	theme	mechanical	378:387	arg1	strategy					430:437	alternative strategy	418:437	alternative strategy to induce chondrogenesis excluding the use of exogenous factors	418:501	Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors.
28332587	1	48	theme	expansion	276:284	arg1	capacity					286:293	chondrocytes' poor expansion capacity	257:293	chondrocytes' poor expansion capacity	257:293	Autologous chondrocyte implantation for cartilage repair represents a challenge because strongly limited by chondrocytes' poor expansion capacity in vitro.
28332587	5	49	theme	MC	730:731	arg1	hydrogel					733:740	MC hydrogel	730:740	MC hydrogel	730:740	MC hydrogel was obtained by dispersion technique and its thermo-reversibility, mechanical properties, degradation and swelling were investigated, demonstrating a solution-gelation transition between 34 and 37 °C and a low bulk degradation (<20%) after 1 month.
28332587	2	50	theme	stem	317:320	arg1	MSCs					329:332	MSCs	329:332	MSCs	329:332	Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors.
28332587	2	50	theme	stem	317:320	arg1	cells					322:326	Mesenchymal stem cells	305:326	Mesenchymal stem cells (MSCs)	305:333	Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors.
28332587	8	51	from	days	1307:1310	arg1	bioreactor					1329:1338	a custom made bioreactor	1315:1338	a custom made bioreactor	1315:1338	PU-MC composites were subjected to a combination of compression and shear forces for 21 days in a custom made bioreactor.
28332587	4	52	theme	Na2SO4	713:718	arg1	solution					720:727	a 0.05 M Na2SO4 solution	704:727	a 0.05 M Na2SO4 solution	704:727	Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution.
28332587	8	53	theme	made	1324:1327	arg1	bioreactor					1329:1338	a custom made bioreactor	1315:1338	a custom made bioreactor	1315:1338	PU-MC composites were subjected to a combination of compression and shear forces for 21 days in a custom made bioreactor.
28332587	6	54	theme	subcutaneous	1072:1083	arg1	implantation					1085:1096	mice subcutaneous implantation	1067:1096	mice subcutaneous implantation	1067:1096	The lack of any hydrogel-derived immunoreaction was demonstrated in vivo by mice subcutaneous implantation.
28332587	2	55	theme	Mesenchymal	305:315	arg1	MSCs					329:332	MSCs	329:332	MSCs	329:332	Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors.
28332587	2	55	theme	Mesenchymal	305:315	arg1	cells					322:326	Mesenchymal stem cells	305:326	Mesenchymal stem cells (MSCs)	305:333	Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors.
28332587	2	56	theme	alternative	418:428	arg1	loading					389:395	mechanical loading	378:395	mechanical loading	378:395	Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors.
28332587	2	56	theme	alternative	418:428	arg1	strategy					430:437	alternative strategy	418:437	alternative strategy to induce chondrogenesis excluding the use of exogenous factors	418:501	Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors.
28332587	4	57	theme	%	674:674	arg1	MC					697:698	MC	697:698	MC	697:698	Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution.
28332587	4	57	theme	%	674:674	arg1	methylcellulose					680:694	8% w/v methylcellulose	673:694	8% w/v methylcellulose (MC)	673:699	Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution.
28332587	9	58	theme	sulphated	1464:1472	arg1	glycosaminoglycans					1474:1491	sulphated glycosaminoglycans	1464:1491	sulphated glycosaminoglycans	1464:1491	Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
28332587	7	59	theme	PU	1207:1208	arg1	matrix					1211:1216	a porous polyurethane (PU) matrix	1184:1216	a porous polyurethane (PU) matrix	1184:1216	To induce in vitro chondrogenesis, MSCs were seeded into MC solution retained within a porous polyurethane (PU) matrix.
28332587	3	60	theme	active	579:584	arg1	interaction					586:596	an active interaction	576:596	an active interaction with cells	576:607	Moreover, MSC supporting material selection is fundamental to allow for an active interaction with cells.
28332587	4	61	theme	8	673:673	arg1	%					674:674	%	674:674	%	674:674	Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution.
25463320	5	0	theme	Pluronic	800:807	arg1	F127					809:812	Pluronic F127	800:812	Pluronic F127	800:812	Studies indicated 8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan as optimum concentration.
25463320	4	1	theme	%	742:742	arg1	binding					750:756	only the % mucin binding	733:756	only the % mucin binding	733:756	The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding.
25463320	6	2	theme	optimized	895:903	arg1	batch					905:909	the optimized batch	891:909	the optimized batch	891:909	Finally, the optimized batch was characterized by polarized light microscopy, small-angle X-ray scattering (SAXS) and transmission electron microscopy.
25463320	6	3	theme	transmission	1000:1011	arg1	microscopy					1022:1031	transmission electron microscopy	1000:1031	transmission electron microscopy	1000:1031	Finally, the optimized batch was characterized by polarized light microscopy, small-angle X-ray scattering (SAXS) and transmission electron microscopy.
25463320	4	4	theme	particle	636:643	arg1	size					645:648	particle size	636:648	particle size	636:648	The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding.
25463320	0	5	theme	clotrimazole	90:101	arg1	nanoparticles					73:85	chitosan-tailored cubic nanoparticles	49:85	chitosan-tailored cubic nanoparticles of clotrimazole	49:101	Optimization, characterization and evaluation of chitosan-tailored cubic nanoparticles of clotrimazole.
25463320	6	6	theme	polarized	932:940	arg1	microscopy					948:957	polarized light microscopy	932:957	polarized light microscopy	932:957	Finally, the optimized batch was characterized by polarized light microscopy, small-angle X-ray scattering (SAXS) and transmission electron microscopy.
25463320	1	7	theme	monoolein	179:187	arg1	nanoparticles					201:213	monoolein based cubic nanoparticles	179:213	monoolein based cubic nanoparticles	179:213	The present study deals with improvement of the mucoadhesive properties of monoolein based cubic nanoparticles by incorporating chitosan.
25463320	9	8	theme	evaluation	1266:1275	arg1	studies					1277:1283	comparative evaluation studies	1254:1283	comparative evaluation studies	1254:1283	Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
25463320	3	9	theme	fraction	411:418	arg1	effect					387:392	The effect	383:392	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations	383:505	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	9	10	theme	nanoparticles	1381:1393	arg1	activity					1325:1332	significantly higher anti-fungal activity	1292:1332	significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles	1292:1393	Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
25463320	3	11	dep	2-factor	525:532	arg1	3-level					535:541	3-level	535:541	3-level	535:541	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	3	11	dep	2-factor	525:532	arg1	central					544:550	central	544:550	central	544:550	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	6	12	theme	electron	1013:1020	arg1	microscopy					1022:1031	transmission electron microscopy	1000:1031	transmission electron microscopy	1000:1031	Finally, the optimized batch was characterized by polarized light microscopy, small-angle X-ray scattering (SAXS) and transmission electron microscopy.
25463320	7	13	theme	chitosan	1072:1079	arg1	incorporation					1055:1067	incorporation	1055:1067	incorporation of chitosan	1055:1079	The results unveiled incorporation of chitosan did not disrupt the inner cubic structure of nanoparticles.
25463320	5	14	theme	optimum	859:865	arg1	concentration					867:879	optimum concentration	859:879	optimum concentration	859:879	Studies indicated 8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan as optimum concentration.
25463320	3	15	theme	Pluronic	397:404	arg1	fraction					411:418	Pluronic F127 fraction	397:418	Pluronic F127 fraction	397:418	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	9	16	theme	conventional	1400:1411	arg1	suspension					1413:1422	conventional suspension	1400:1422	conventional suspension of clotrimazole against Candida albicans	1400:1463	Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
25463320	7	17	theme	nanoparticles	1126:1138	arg1	structure					1113:1121	the inner cubic structure	1097:1121	the inner cubic structure of nanoparticles	1097:1138	The results unveiled incorporation of chitosan did not disrupt the inner cubic structure of nanoparticles.
25463320	4	18	theme	cubic	673:677	arg1	nanoparticles					679:691	cubic nanoparticles	673:691	cubic nanoparticles	673:691	The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding.
25463320	9	19	theme	cubic	1375:1379	arg1	nanoparticles					1381:1393	clotrimazole-loaded chitosan-tailored cubic nanoparticles	1337:1393	clotrimazole-loaded chitosan-tailored cubic nanoparticles	1337:1393	Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
25463320	3	20	theme	mucin	473:477	arg1	binding					479:485	% mucin binding	471:485	% mucin binding	471:485	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	1	21	theme	based	189:193	arg1	nanoparticles					201:213	monoolein based cubic nanoparticles	179:213	monoolein based cubic nanoparticles	179:213	The present study deals with improvement of the mucoadhesive properties of monoolein based cubic nanoparticles by incorporating chitosan.
25463320	3	22	theme	F127	406:409	arg1	fraction					411:418	Pluronic F127 fraction	397:418	Pluronic F127 fraction	397:418	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	7	23	dep	unveiled	1046:1053	arg1	disrupt					1089:1095	disrupt	1089:1095	unveiled incorporation of chitosan did not disrupt the inner cubic structure of nanoparticles	1046:1138	The results unveiled incorporation of chitosan did not disrupt the inner cubic structure of nanoparticles.
25463320	1	24	theme	cubic	195:199	arg1	nanoparticles					201:213	monoolein based cubic nanoparticles	179:213	monoolein based cubic nanoparticles	179:213	The present study deals with improvement of the mucoadhesive properties of monoolein based cubic nanoparticles by incorporating chitosan.
25463320	3	25	theme	chitosan	441:448	arg1	concentration					424:436	concentration	424:436	concentration of chitosan	424:448	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	3	25	theme	chitosan	441:448	arg1	fraction					411:418	Pluronic F127 fraction	397:418	Pluronic F127 fraction	397:418	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	5	26	theme	F127	809:812	arg1	fraction					788:795	8.33(%w/w) fraction	777:795	8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan	777:854	Studies indicated 8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan as optimum concentration.
25463320	1	27	theme	nanoparticles	201:213	arg1	properties					165:174	the mucoadhesive properties	148:174	the mucoadhesive properties of monoolein based cubic nanoparticles	148:213	The present study deals with improvement of the mucoadhesive properties of monoolein based cubic nanoparticles by incorporating chitosan.
25463320	8	28	theme	cubic	1214:1218	arg1	phases					1220:1225	P-type and D-type cubic phases	1196:1225	P-type and D-type cubic phases	1196:1225	Peak indexing of SAXS data revealed the coexistence of P-type and D-type cubic phases in nanoparticles.
25463320	9	29	theme	clotrimazole	1427:1438	arg1	suspension					1413:1422	conventional suspension	1400:1422	conventional suspension of clotrimazole against Candida albicans	1400:1463	Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
25463320	1	30	theme	present	108:114	arg1	study					116:120	The present study	104:120	The present study	104:120	The present study deals with improvement of the mucoadhesive properties of monoolein based cubic nanoparticles by incorporating chitosan.
25463320	2	31	dep	film	302:305	arg1	hydration					307:315	hydration	307:315	thin film hydration followed by ultrasonication employing clotrimazole as model drug	297:380	Chitosan-tailored cubic nanoparticles were prepared by thin film hydration followed by ultrasonication employing clotrimazole as model drug.
25463320	3	32	theme	concentration	424:436	arg1	effect					387:392	The effect	383:392	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations	383:505	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	4	33	theme	chitosan	604:611	arg1	concentration					587:599	The concentration	583:599	The concentration of chitosan	583:611	The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding.
25463320	7	34	theme	inner	1101:1105	arg1	structure					1113:1121	the inner cubic structure	1097:1121	the inner cubic structure of nanoparticles	1097:1138	The results unveiled incorporation of chitosan did not disrupt the inner cubic structure of nanoparticles.
25463320	9	35	theme	comparative	1254:1264	arg1	studies					1277:1283	comparative evaluation studies	1254:1283	comparative evaluation studies	1254:1283	Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
25463320	4	36	theme	nanoparticles	679:691	arg1	size					645:648	particle size	636:648	particle size	636:648	The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding.
25463320	4	36	theme	nanoparticles	679:691	arg1	binding					662:668	% mucin binding	654:668	% mucin binding	654:668	The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding.
25463320	2	37	theme	model	371:375	arg1	drug					377:380	model drug	371:380	model drug	371:380	Chitosan-tailored cubic nanoparticles were prepared by thin film hydration followed by ultrasonication employing clotrimazole as model drug.
25463320	9	38	theme	Candida	1448:1454	arg1	albicans					1456:1463	Candida albicans	1448:1463	Candida albicans	1448:1463	Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
25463320	7	39	theme	cubic	1107:1111	arg1	structure					1113:1121	the inner cubic structure	1097:1121	the inner cubic structure of nanoparticles	1097:1138	The results unveiled incorporation of chitosan did not disrupt the inner cubic structure of nanoparticles.
25463320	8	40	from	coexistence	1181:1191	arg1	nanoparticles					1230:1242	nanoparticles	1230:1242	nanoparticles	1230:1242	Peak indexing of SAXS data revealed the coexistence of P-type and D-type cubic phases in nanoparticles.
25463320	3	41	theme	composite	552:560	arg1	design					575:580	2-factor, 3-level, central composite experimental design	525:580	2-factor, 3-level, central composite experimental design	525:580	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	6	42	theme	light	942:946	arg1	microscopy					948:957	polarized light microscopy	932:957	polarized light microscopy	932:957	Finally, the optimized batch was characterized by polarized light microscopy, small-angle X-ray scattering (SAXS) and transmission electron microscopy.
25463320	3	43	theme	experimental	562:573	arg1	design					575:580	2-factor, 3-level, central composite experimental design	525:580	2-factor, 3-level, central composite experimental design	525:580	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	5	44	dep	fraction	788:795	arg1	%					782:782	%	782:782	%w/w	782:785	Studies indicated 8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan as optimum concentration.
25463320	4	45	theme	Pluronic	699:706	arg1	fraction					713:720	Pluronic F127 fraction	699:720	Pluronic F127 fraction	699:720	The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding.
25463320	3	46	theme	formulations	494:505	arg1	size					462:465	particle size	453:465	particle size	453:465	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	3	46	theme	formulations	494:505	arg1	binding					479:485	% mucin binding	471:485	% mucin binding	471:485	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	3	47	theme	%	471:471	arg1	binding					479:485	% mucin binding	471:485	% mucin binding	471:485	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	5	48	theme	0.17	818:821	arg1	concentration					830:842	0.17 (%w/v) concentration	818:842	0.17 (%w/v) concentration	818:842	Studies indicated 8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan as optimum concentration.
25463320	5	49	theme	concentration	830:842	arg1	fraction					788:795	8.33(%w/w) fraction	777:795	8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan	777:854	Studies indicated 8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan as optimum concentration.
25463320	8	50	theme	phases	1220:1225	arg1	coexistence					1181:1191	the coexistence	1177:1191	the coexistence of P-type and D-type cubic phases in nanoparticles	1177:1242	Peak indexing of SAXS data revealed the coexistence of P-type and D-type cubic phases in nanoparticles.
25463320	3	51	theme	particle	453:460	arg1	size					462:465	particle size	453:465	particle size	453:465	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	6	52	theme	X-ray	972:976	arg1	SAXS					990:993	SAXS	990:993	SAXS	990:993	Finally, the optimized batch was characterized by polarized light microscopy, small-angle X-ray scattering (SAXS) and transmission electron microscopy.
25463320	6	52	theme	X-ray	972:976	arg1	scattering					978:987	small-angle X-ray scattering	960:987	small-angle X-ray scattering (SAXS)	960:994	Finally, the optimized batch was characterized by polarized light microscopy, small-angle X-ray scattering (SAXS) and transmission electron microscopy.
25463320	5	53	theme	%	824:824	arg1	0.17					818:821	0.17	818:821	0.17	818:821	Studies indicated 8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan as optimum concentration.
25463320	5	53	theme	%	824:824	arg1	w/v					825:827	%w/v	824:827	%w/v	824:827	Studies indicated 8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan as optimum concentration.
25463320	0	54	theme	nanoparticles	73:85	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Optimization, characterization and evaluation of chitosan-tailored cubic nanoparticles of clotrimazole.
25463320	0	54	theme	nanoparticles	73:85	arg1	Optimization					0:11	Optimization	0:11	Optimization	0:11	Optimization, characterization and evaluation of chitosan-tailored cubic nanoparticles of clotrimazole.
25463320	0	54	theme	nanoparticles	73:85	arg1	evaluation					35:44	evaluation	35:44	evaluation	35:44	Optimization, characterization and evaluation of chitosan-tailored cubic nanoparticles of clotrimazole.
25463320	0	55	theme	chitosan-tailored	49:65	arg1	nanoparticles					73:85	chitosan-tailored cubic nanoparticles	49:85	chitosan-tailored cubic nanoparticles of clotrimazole	49:101	Optimization, characterization and evaluation of chitosan-tailored cubic nanoparticles of clotrimazole.
25463320	6	56	theme	small-angle	960:970	arg1	SAXS					990:993	SAXS	990:993	SAXS	990:993	Finally, the optimized batch was characterized by polarized light microscopy, small-angle X-ray scattering (SAXS) and transmission electron microscopy.
25463320	6	56	theme	small-angle	960:970	arg1	scattering					978:987	small-angle X-ray scattering	960:987	small-angle X-ray scattering (SAXS)	960:994	Finally, the optimized batch was characterized by polarized light microscopy, small-angle X-ray scattering (SAXS) and transmission electron microscopy.
25463320	8	57	theme	Peak	1141:1144	arg1	indexing					1146:1153	Peak indexing	1141:1153	Peak indexing of SAXS data	1141:1166	Peak indexing of SAXS data revealed the coexistence of P-type and D-type cubic phases in nanoparticles.
25463320	2	58	theme	Chitosan-tailored	242:258	arg1	nanoparticles					266:278	Chitosan-tailored cubic nanoparticles	242:278	Chitosan-tailored cubic nanoparticles	242:278	Chitosan-tailored cubic nanoparticles were prepared by thin film hydration followed by ultrasonication employing clotrimazole as model drug.
25463320	2	59	theme	cubic	260:264	arg1	nanoparticles					266:278	Chitosan-tailored cubic nanoparticles	242:278	Chitosan-tailored cubic nanoparticles	242:278	Chitosan-tailored cubic nanoparticles were prepared by thin film hydration followed by ultrasonication employing clotrimazole as model drug.
25463320	9	60	theme	chitosan-tailored	1357:1373	arg1	nanoparticles					1381:1393	clotrimazole-loaded chitosan-tailored cubic nanoparticles	1337:1393	clotrimazole-loaded chitosan-tailored cubic nanoparticles	1337:1393	Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
25463320	8	61	theme	SAXS	1158:1161	arg1	data					1163:1166	SAXS data	1158:1166	SAXS data	1158:1166	Peak indexing of SAXS data revealed the coexistence of P-type and D-type cubic phases in nanoparticles.
25463320	9	62	theme	anti-fungal	1313:1323	arg1	activity					1325:1332	significantly higher anti-fungal activity	1292:1332	significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles	1292:1393	Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
25463320	3	63	theme	2-factor	525:532	arg1	design					575:580	2-factor, 3-level, central composite experimental design	525:580	2-factor, 3-level, central composite experimental design	525:580	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	4	64	theme	mucin	744:748	arg1	binding					750:756	only the % mucin binding	733:756	only the % mucin binding	733:756	The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding.
25463320	4	65	theme	mucin	656:660	arg1	binding					662:668	% mucin binding	654:668	% mucin binding	654:668	The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding.
25463320	5	66	dep	%	782:782	arg1	w/w					783:785	w/w	783:785	%w/w	782:785	Studies indicated 8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan as optimum concentration.
25463320	3	67	from	effect	387:392	arg1	size					462:465	particle size	453:465	particle size	453:465	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	3	67	from	effect	387:392	arg1	binding					479:485	% mucin binding	471:485	% mucin binding	471:485	The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design.
25463320	9	68	theme	higher	1306:1311	arg1	activity					1325:1332	significantly higher anti-fungal activity	1292:1332	significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles	1292:1393	Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
25463320	8	69	theme	data	1163:1166	arg1	indexing					1146:1153	Peak indexing	1141:1153	Peak indexing of SAXS data	1141:1166	Peak indexing of SAXS data revealed the coexistence of P-type and D-type cubic phases in nanoparticles.
25463320	4	70	theme	%	654:654	arg1	binding					662:668	% mucin binding	654:668	% mucin binding	654:668	The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding.
25463320	2	71	theme	thin	297:300	arg1	film					302:305	thin film	297:305	thin film hydration followed by ultrasonication employing clotrimazole as model drug	297:380	Chitosan-tailored cubic nanoparticles were prepared by thin film hydration followed by ultrasonication employing clotrimazole as model drug.
25463320	5	72	theme	chitosan	847:854	arg1	fraction					788:795	8.33(%w/w) fraction	777:795	8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan	777:854	Studies indicated 8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan as optimum concentration.
25463320	1	73	theme	mucoadhesive	152:163	arg1	properties					165:174	the mucoadhesive properties	148:174	the mucoadhesive properties of monoolein based cubic nanoparticles	148:213	The present study deals with improvement of the mucoadhesive properties of monoolein based cubic nanoparticles by incorporating chitosan.
25463320	4	74	theme	F127	708:711	arg1	fraction					713:720	Pluronic F127 fraction	699:720	Pluronic F127 fraction	699:720	The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding.
25463320	8	75	theme	P-type	1196:1201	arg1	phases					1220:1225	P-type and D-type cubic phases	1196:1225	P-type and D-type cubic phases	1196:1225	Peak indexing of SAXS data revealed the coexistence of P-type and D-type cubic phases in nanoparticles.
25463320	0	76	theme	cubic	67:71	arg1	nanoparticles					73:85	chitosan-tailored cubic nanoparticles	49:85	chitosan-tailored cubic nanoparticles of clotrimazole	49:101	Optimization, characterization and evaluation of chitosan-tailored cubic nanoparticles of clotrimazole.
25463320	8	77	theme	D-type	1207:1212	arg1	phases					1220:1225	P-type and D-type cubic phases	1196:1225	P-type and D-type cubic phases	1196:1225	Peak indexing of SAXS data revealed the coexistence of P-type and D-type cubic phases in nanoparticles.
25463320	9	78	theme	clotrimazole-loaded	1337:1355	arg1	nanoparticles					1381:1393	clotrimazole-loaded chitosan-tailored cubic nanoparticles	1337:1393	clotrimazole-loaded chitosan-tailored cubic nanoparticles	1337:1393	Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
25463320	1	79	theme	properties	165:174	arg1	improvement					133:143	improvement	133:143	improvement of the mucoadhesive properties of monoolein based cubic nanoparticles	133:213	The present study deals with improvement of the mucoadhesive properties of monoolein based cubic nanoparticles by incorporating chitosan.
28848343	6	0	theme	plasma	1197:1202	arg1	spectrometry					1221:1232	inductively coupled plasma optical emission spectrometry	1177:1232	inductively coupled plasma optical emission spectrometry	1177:1232	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	6	0	theme	plasma	1197:1202	arg1	selenium					1167:1174	selenium	1167:1174	selenium (inductively coupled plasma optical emission spectrometry)	1167:1233	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	11	1	theme	release	1927:1933	arg1	"					1934:1934	the "burst release"	1916:1934	the "burst release" of selenium during the first 10 h	1916:1968	However, because of the "burst release" of selenium during the first 10 h, the granules significantly reduced viability of normal osteoblasts as well.
28848343	6	2	theme	selenium	1167:1174	arg1	release					1156:1162	the release	1152:1162	the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules	1152:1301	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	4	3	theme	obtained	743:750	arg1	microgranules					762:774	the obtained spherical microgranules	739:774	the obtained spherical microgranules of a size of about 1.1-1.5 mm	739:804	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	6	4	theme	liquid	1261:1266	arg1	RIS					1239:1241	RIS	1239:1241	RIS (high-performance liquid chromatography)	1239:1282	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	6	4	theme	liquid	1261:1266	arg1	chromatography					1268:1281	high-performance liquid chromatography	1244:1281	high-performance liquid chromatography	1244:1281	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	3	5	theme	hydroxyapatite/alginate	641:663	arg1	granules					686:693	the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules	617:693	the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules	617:693	SeHA was then used to develop the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules.
28848343	4	6	theme	granules	932:939	arg1	result					913:918	a result	911:918	a result of SeHA/ALG granules soaking in aqueous RIS solution	911:971	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	4	6	theme	granules	932:939	arg1	sodium					708:713	Risedronate sodium	696:713	Risedronate sodium (RIS)	696:719	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	8	7	theme	Selenium	1389:1396	arg1	release					1398:1404	Selenium release	1389:1404	Selenium release	1389:1404	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	2	8	theme	careful	306:312	arg1	analysis					330:337	careful physicochemical analysis	306:337	careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry	306:584	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	6	9	theme	high-performance	1244:1259	arg1	RIS					1239:1241	RIS	1239:1241	RIS (high-performance liquid chromatography)	1239:1282	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	6	9	theme	high-performance	1244:1259	arg1	chromatography					1268:1281	high-performance liquid chromatography	1244:1281	high-performance liquid chromatography	1244:1281	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	0	10	theme	bone	160:163	arg1	tumors					165:170	bone tumors	160:170	bone tumors	160:170	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	5	11	theme	RIS	1103:1105	arg1	interaction					1115:1125	its interaction	1111:1125	its interaction with calcium ions	1111:1143	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	5	11	theme	RIS	1103:1105	arg1	presence					1091:1098	the presence	1087:1098	the presence of RIS	1087:1105	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	2	12	theme	selenium	248:255	arg1	selenium					248:255	selenium)	248:256	selenium)	248:256	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	2	12	theme	selenium	248:255	arg1	%					243:243	%	243:243	% of selenium)	243:256	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	9	13	theme	obtaining	1696:1704	arg1	microgranules					1706:1718	obtaining microgranules	1696:1718	obtaining microgranules containing RIS	1696:1733	The study showed that the method of obtaining microgranules containing RIS significantly affects its release profile.
28848343	6	14	theme	RIS	1239:1241	arg1	release					1156:1162	the release	1152:1162	the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules	1152:1301	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	5	15	theme	magnetic	1058:1065	arg1	resonance					1067:1075	spinning nuclear magnetic resonance	1041:1075	spinning nuclear magnetic resonance	1041:1075	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	3	16	theme	SeHA/ALG	666:673	arg1	granules					686:693	the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules	617:693	the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules	617:693	SeHA was then used to develop the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules.
28848343	8	17	theme	release	1471:1477	arg1	"					1478:1478	a "burst release"	1462:1478	a "burst release" probably from a hydrated surface layer of SeHA crystals	1462:1534	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	10	18	theme	high	1844:1847	arg1	activity					1859:1866	high antitumor activity	1844:1866	high antitumor activity	1844:1866	Performed cytotoxicity test revealed that fabricated granules had high antitumor activity against osteosarcoma cells.
28848343	2	19	theme	optical	556:562	arg1	spectrometry					573:584	inductively coupled plasma optical emission spectrometry	529:584	inductively coupled plasma optical emission spectrometry	529:584	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	6	20	theme	emission	1212:1219	arg1	spectrometry					1221:1232	inductively coupled plasma optical emission spectrometry	1177:1232	inductively coupled plasma optical emission spectrometry	1177:1232	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	6	20	theme	emission	1212:1219	arg1	selenium					1167:1174	selenium	1167:1174	selenium (inductively coupled plasma optical emission spectrometry)	1167:1233	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	5	21	with	interaction	1115:1125	arg1	ions					1140:1143	calcium ions	1132:1143	calcium ions	1132:1143	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	1	22	theme	Nanocrystalline	173:187	arg1	hydroxyapatite					189:202	Nanocrystalline hydroxyapatite	173:202	Nanocrystalline hydroxyapatite	173:202	Nanocrystalline hydroxyapatite containing selenite ions (SeHA; 9.6 wt.
28848343	2	23	theme	coupled	541:547	arg1	spectrometry					573:584	inductively coupled plasma optical emission spectrometry	529:584	inductively coupled plasma optical emission spectrometry	529:584	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	4	24	theme	RIS	960:962	arg1	solution					964:971	aqueous RIS solution	952:971	aqueous RIS solution	952:971	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	8	25	theme	surface	1505:1511	arg1	layer					1513:1517	a hydrated surface layer	1494:1517	a hydrated surface layer of SeHA crystals	1494:1534	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	2	26	dep	transform	376:384	arg1	infrared					386:393	infrared	386:393	transform infrared spectroscopy	376:406	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	0	27	theme	risedronate	111:121	arg1	sodium					123:128	risedronate sodium	111:128	risedronate sodium	111:128	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	11	28	theme	selenium	1939:1946	arg1	"					1934:1934	the "burst release"	1916:1934	the "burst release" of selenium during the first 10 h	1916:1968	However, because of the "burst release" of selenium during the first 10 h, the granules significantly reduced viability of normal osteoblasts as well.
28848343	2	29	theme	dispersive	494:503	arg1	fluorescence					511:522	wavelength dispersive X-ray fluorescence	483:522	wavelength dispersive X-ray fluorescence	483:522	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	8	30	theme	crystals	1527:1534	arg1	layer					1513:1517	a hydrated surface layer	1494:1517	a hydrated surface layer of SeHA crystals	1494:1534	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	2	31	theme	transmission	409:420	arg1	microscopy					431:440	transmission electron microscopy	409:440	transmission electron microscopy	409:440	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	7	32	theme	granules	1354:1361	arg1	cytotoxicity					1327:1338	cytotoxicity	1327:1338	cytotoxicity of fabricated granules	1327:1361	Moreover, cytotoxicity of fabricated granules was assessed by MTT test.
28848343	2	33	theme	magnetic	463:470	arg1	resonance					472:480	solid-state nuclear magnetic resonance	443:480	solid-state nuclear magnetic resonance	443:480	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	0	34	theme	physicochemical	16:30	arg1	characterization					32:47	physicochemical characterization	16:47	physicochemical characterization	16:47	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	8	35	theme	next	1547:1550	arg1	stage					1552:1556	the next stage	1543:1556	the next stage	1543:1556	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	8	35	theme	next	1547:1550	arg1	longer					1576:1581	longer	1576:1581	longer	1576:1581	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	10	36	theme	osteosarcoma	1876:1887	arg1	cells					1889:1893	osteosarcoma cells	1876:1893	osteosarcoma cells	1876:1893	Performed cytotoxicity test revealed that fabricated granules had high antitumor activity against osteosarcoma cells.
28848343	2	37	theme	solid-state	443:453	arg1	resonance					472:480	solid-state nuclear magnetic resonance	443:480	solid-state nuclear magnetic resonance	443:480	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	0	38	theme	composite	59:67	arg1	microgranules					69:81	porous composite microgranules	52:81	porous composite microgranules with selenium oxyanions and risedronate sodium	52:128	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	10	39	theme	antitumor	1849:1857	arg1	activity					1859:1866	high antitumor activity	1844:1866	high antitumor activity	1844:1866	Performed cytotoxicity test revealed that fabricated granules had high antitumor activity against osteosarcoma cells.
28848343	5	40	theme	nuclear	1050:1056	arg1	resonance					1067:1075	spinning nuclear magnetic resonance	1041:1075	spinning nuclear magnetic resonance	1041:1075	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	6	41	theme	optical	1204:1210	arg1	spectrometry					1221:1232	inductively coupled plasma optical emission spectrometry	1177:1232	inductively coupled plasma optical emission spectrometry	1177:1232	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	6	41	theme	optical	1204:1210	arg1	selenium					1167:1174	selenium	1167:1174	selenium (inductively coupled plasma optical emission spectrometry)	1167:1233	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	2	42	theme	X-ray	349:353	arg1	diffraction					355:365	powder X-ray diffraction	342:365	powder X-ray diffraction	342:365	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	4	43	theme	RIS	851:853	arg1	solution					855:862	RIS solution	851:862	RIS solution added to a suspension of ALG and SeHA	851:900	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	5	44	theme	cross-polarization	1010:1027	arg1	angle					1035:1039	13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance	998:1075	angle	1035:1039	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	4	45	theme	Risedronate	696:706	arg1	result					913:918	a result	911:918	a result of SeHA/ALG granules soaking in aqueous RIS solution	911:971	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	4	45	theme	Risedronate	696:706	arg1	RIS					716:718	RIS	716:718	RIS	716:718	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	4	45	theme	Risedronate	696:706	arg1	sodium					708:713	Risedronate sodium	696:713	Risedronate sodium (RIS)	696:719	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	4	46	theme	SeHA	897:900	arg1	suspension					875:884	a suspension	873:884	a suspension of ALG and SeHA	873:900	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	10	47	theme	cytotoxicity	1788:1799	arg1	test					1801:1804	Performed cytotoxicity test	1778:1804	Performed cytotoxicity test	1778:1804	Performed cytotoxicity test revealed that fabricated granules had high antitumor activity against osteosarcoma cells.
28848343	6	48	theme	coupled	1189:1195	arg1	spectrometry					1221:1232	inductively coupled plasma optical emission spectrometry	1177:1232	inductively coupled plasma optical emission spectrometry	1177:1232	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	6	48	theme	coupled	1189:1195	arg1	selenium					1167:1174	selenium	1167:1174	selenium (inductively coupled plasma optical emission spectrometry)	1167:1233	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	8	49	theme	selenium	1622:1629	arg1	release					1611:1617	a sustained release	1599:1617	a sustained release of selenium from the crystals' interior	1599:1657	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	1	50	theme	selenite	215:222	arg1	ions					224:227	selenite ions	215:227	selenite ions (SeHA; 9.6 wt	215:241	Nanocrystalline hydroxyapatite containing selenite ions (SeHA; 9.6 wt.
28848343	3	51	theme	selenium-containing	621:639	arg1	granules					686:693	the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules	617:693	the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules	617:693	SeHA was then used to develop the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules.
28848343	4	52	dep	preparation	838:848	arg1	solution					855:862	RIS solution	851:862	RIS solution added to a suspension of ALG and SeHA	851:900	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	4	53	theme	ALG	889:891	arg1	suspension					875:884	a suspension	873:884	a suspension of ALG and SeHA	873:900	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	5	54	dep	angle	1035:1039	arg1	resonance					1067:1075	spinning nuclear magnetic resonance	1041:1075	spinning nuclear magnetic resonance	1041:1075	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	8	55	theme	first	1424:1428	arg1	stage					1430:1434	the first stage	1420:1434	the first stage	1420:1434	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	2	56	theme	physicochemical	314:328	arg1	analysis					330:337	careful physicochemical analysis	306:337	careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry	306:584	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	10	57	theme	Performed	1778:1786	arg1	test					1801:1804	Performed cytotoxicity test	1778:1804	Performed cytotoxicity test	1778:1804	Performed cytotoxicity test revealed that fabricated granules had high antitumor activity against osteosarcoma cells.
28848343	4	58	theme	SeHA/ALG	923:930	arg1	granules					932:939	SeHA/ALG granules	923:939	SeHA/ALG granules soaking in aqueous RIS solution	923:971	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	10	59	theme	fabricated	1820:1829	arg1	granules					1831:1838	fabricated granules	1820:1838	fabricated granules	1820:1838	Performed cytotoxicity test revealed that fabricated granules had high antitumor activity against osteosarcoma cells.
28848343	3	60	used	used	601:604	arg2	SeHA					587:590	SeHA	587:590	SeHA	587:590	SeHA was then used to develop the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules.
28848343	8	61	theme	burst	1465:1469	arg1	"					1478:1478	a "burst release"	1462:1478	a "burst release" probably from a hydrated surface layer of SeHA crystals	1462:1534	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	9	62	theme	microgranules	1706:1718	arg1	method					1686:1691	the method	1682:1691	the method of obtaining microgranules containing RIS	1682:1733	The study showed that the method of obtaining microgranules containing RIS significantly affects its release profile.
28848343	4	63	theme	size	781:784	arg1	microgranules					762:774	the obtained spherical microgranules	739:774	the obtained spherical microgranules of a size of about 1.1-1.5 mm	739:804	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	2	64	theme	wet	280:282	arg1	method					284:289	wet method	280:289	wet method	280:289	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	11	65	theme	normal	2019:2024	arg1	osteoblasts					2026:2036	normal osteoblasts	2019:2036	normal osteoblasts	2019:2036	However, because of the "burst release" of selenium during the first 10 h, the granules significantly reduced viability of normal osteoblasts as well.
28848343	8	66	from	interior	1650:1657	arg1	release					1611:1617	a sustained release	1599:1617	a sustained release of selenium from the crystals' interior	1599:1657	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	0	67	theme	selenium	88:95	arg1	oxyanions					97:105	selenium oxyanions	88:105	selenium oxyanions	88:105	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	2	68	theme	plasma	549:554	arg1	spectrometry					573:584	inductively coupled plasma optical emission spectrometry	529:584	inductively coupled plasma optical emission spectrometry	529:584	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	8	69	theme	hydrated	1496:1503	arg1	layer					1513:1517	a hydrated surface layer	1494:1517	a hydrated surface layer of SeHA crystals	1494:1534	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	7	70	theme	MTT	1379:1381	arg1	test					1383:1386	MTT test	1379:1386	MTT test	1379:1386	Moreover, cytotoxicity of fabricated granules was assessed by MTT test.
28848343	4	71	theme	spherical	752:760	arg1	microgranules					762:774	the obtained spherical microgranules	739:774	the obtained spherical microgranules of a size of about 1.1-1.5 mm	739:804	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	9	72	theme	release	1761:1767	arg1	profile					1769:1775	its release profile	1757:1775	its release profile	1757:1775	The study showed that the method of obtaining microgranules containing RIS significantly affects its release profile.
28848343	11	73	theme	osteoblasts	2026:2036	arg1	viability					2006:2014	viability	2006:2014	viability of normal osteoblasts	2006:2036	However, because of the "burst release" of selenium during the first 10 h, the granules significantly reduced viability of normal osteoblasts as well.
28848343	4	74	theme	aqueous	952:958	arg1	solution					964:971	aqueous RIS solution	952:971	aqueous RIS solution	952:971	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	8	75	theme	SeHA	1522:1525	arg1	crystals					1527:1534	SeHA crystals	1522:1534	SeHA crystals	1522:1534	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	2	76	dep	Fourier	368:374	arg1	transform					376:384	transform	376:384	transform infrared spectroscopy	376:406	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	0	77	theme	potential	134:142	arg1	applications					144:155	potential applications	134:155	potential applications in bone tumors	134:170	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	2	78	theme	emission	564:571	arg1	spectrometry					573:584	inductively coupled plasma optical emission spectrometry	529:584	inductively coupled plasma optical emission spectrometry	529:584	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	2	79	theme	electron	422:429	arg1	microscopy					431:440	transmission electron microscopy	409:440	transmission electron microscopy	409:440	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	8	80	from	layer	1513:1517	arg1	"					1478:1478	a "burst release"	1462:1478	a "burst release" probably from a hydrated surface layer of SeHA crystals	1462:1534	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	5	81	theme	calcium	1132:1138	arg1	ions					1140:1143	calcium ions	1132:1143	calcium ions	1132:1143	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	7	82	theme	fabricated	1343:1352	arg1	granules					1354:1361	fabricated granules	1343:1361	fabricated granules	1343:1361	Moreover, cytotoxicity of fabricated granules was assessed by MTT test.
28848343	2	83	theme	wavelength	483:492	arg1	fluorescence					511:522	wavelength dispersive X-ray fluorescence	483:522	wavelength dispersive X-ray fluorescence	483:522	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	4	84	theme	mm	803:804	arg1	size					781:784	a size	779:784	a size of about 1.1-1.5 mm	779:804	Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1-1.5 mm in 2 ways: during the granules' preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution.
28848343	11	85	theme	first	1959:1963	arg1	h					1968:1968	the first 10 h	1955:1968	the first 10 h	1955:1968	However, because of the "burst release" of selenium during the first 10 h, the granules significantly reduced viability of normal osteoblasts as well.
28848343	5	86	with	presence	1091:1098	arg1	ions					1140:1143	calcium ions	1132:1143	calcium ions	1132:1143	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	2	87	theme	nuclear	455:461	arg1	resonance					472:480	solid-state nuclear magnetic resonance	443:480	solid-state nuclear magnetic resonance	443:480	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	0	88	theme	porous	52:57	arg1	microgranules					69:81	porous composite microgranules	52:81	porous composite microgranules with selenium oxyanions and risedronate sodium	52:128	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	5	89	theme	magic	1029:1033	arg1	angle					1035:1039	13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance	998:1075	angle	1035:1039	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	10	90	contain	had	1840:1842	arg2	activity					1859:1866	high antitumor activity	1844:1866	high antitumor activity	1844:1866	Performed cytotoxicity test revealed that fabricated granules had high antitumor activity against osteosarcoma cells.
28848343	10	90	contain	had	1840:1842	arg1	granules					1831:1838	fabricated granules	1820:1838	fabricated granules	1820:1838	Performed cytotoxicity test revealed that fabricated granules had high antitumor activity against osteosarcoma cells.
28848343	1	91	dep	SeHA	230:233	arg1	wt					240:241	9.6 wt	236:241	SeHA; 9.6 wt	230:241	Nanocrystalline hydroxyapatite containing selenite ions (SeHA; 9.6 wt.
28848343	0	92	theme	microgranules	69:81	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	0	92	theme	microgranules	69:81	arg1	characterization					32:47	physicochemical characterization	16:47	physicochemical characterization	16:47	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	5	93	theme	spinning	1041:1048	arg1	resonance					1067:1075	spinning nuclear magnetic resonance	1041:1075	spinning nuclear magnetic resonance	1041:1075	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	2	94	theme	X-ray	505:509	arg1	fluorescence					511:522	wavelength dispersive X-ray fluorescence	483:522	wavelength dispersive X-ray fluorescence	483:522	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	1	95	dep	ions	224:227	arg1	SeHA					230:233	SeHA	230:233	SeHA; 9.6 wt	230:241	Nanocrystalline hydroxyapatite containing selenite ions (SeHA; 9.6 wt.
28848343	5	96	theme	13C	998:1000	arg1	angle					1035:1039	13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance	998:1075	angle	1035:1039	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	3	97	theme	composite	676:684	arg1	granules					686:693	the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules	617:693	the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules	617:693	SeHA was then used to develop the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules.
28848343	0	98	with	microgranules	69:81	arg1	oxyanions					97:105	selenium oxyanions	88:105	selenium oxyanions	88:105	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	0	98	with	microgranules	69:81	arg1	sodium					123:128	risedronate sodium	111:128	risedronate sodium	111:128	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	2	99	theme	powder	342:347	arg1	diffraction					355:365	powder X-ray diffraction	342:365	powder X-ray diffraction	342:365	% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry.
28848343	6	100	from	microgranules	1289:1301	arg1	release					1156:1162	the release	1152:1162	the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules	1152:1301	Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined.
28848343	5	101	theme	31P	1006:1008	arg1	angle					1035:1039	13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance	998:1075	angle	1035:1039	The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions.
28848343	11	102	theme	burst	1921:1925	arg1	"					1934:1934	the "burst release"	1916:1934	the "burst release" of selenium during the first 10 h	1916:1968	However, because of the "burst release" of selenium during the first 10 h, the granules significantly reduced viability of normal osteoblasts as well.
28848343	8	103	theme	sustained	1601:1609	arg1	release					1611:1617	a sustained release	1599:1617	a sustained release of selenium from the crystals' interior	1599:1657	Selenium release was biphasic: the first stage was short and ascribed to a "burst release" probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals' interior.
28848343	0	104	from	applications	144:155	arg1	tumors					165:170	bone tumors	160:170	bone tumors	160:170	Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors.
28848343	9	105	contain	containing	1720:1729	arg2	RIS					1731:1733	RIS	1731:1733	RIS	1731:1733	The study showed that the method of obtaining microgranules containing RIS significantly affects its release profile.
28848343	9	105	contain	containing	1720:1729	arg1	microgranules					1706:1718	obtaining microgranules	1696:1718	obtaining microgranules containing RIS	1696:1733	The study showed that the method of obtaining microgranules containing RIS significantly affects its release profile.
27481928	2	0	theme	possible	611:618	arg1	associations					620:631	possible associations	611:631	possible associations between LOS locus class sequence diversity and biochemical heterogeneity	611:704	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	1	1	theme	coli	438:441	arg1	isolates					443:450	unrelated C. coli isolates	425:450	unrelated C. coli isolates sampled from several different host species	425:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	8	2	theme	LOS	2152:2154	arg1	composition					2165:2175	LOS chemical composition	2152:2175	LOS chemical composition	2152:2175	Therefore, we paired C. coli genomic information and LOS chemical composition for the first time to address this paucity of information.
27481928	5	3	theme	A	1234:1234	arg1	backbone					1236:1243	the lipid A backbone	1224:1243	the lipid A backbone	1224:1243	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	7	4	theme	syndrome	2013:2020	arg1	agent					1989:1993	a causative agent	1977:1993	a causative agent of Guillain-Barré syndrome	1977:2020	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	2	5	theme	locus	645:649	arg1	diversity					666:674	LOS locus class sequence diversity	641:674	LOS locus class sequence diversity	641:674	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	5	6	attach	present	1213:1219	arg2	disaccharides					1194:1206	2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides	1156:1206	2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides	1156:1206	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	5	6	attach	present	1213:1219	arg1	backbone					1236:1243	the lipid A backbone	1224:1243	the lipid A backbone	1224:1243	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	5	7	theme	C.	1594:1595	arg1	isolates					1602:1609	all C. coli isolates	1590:1609	all C. coli isolates	1590:1609	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	3	8	theme	locus	739:743	arg1	classes					745:751	three new LOS locus classes	725:751	three new LOS locus classes	725:751	After identifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought.
27481928	5	9	from	present	1213:1219	arg1	backbone					1236:1243	the lipid A backbone	1224:1243	the lipid A backbone	1224:1243	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	1	10	theme	several	465:471	arg1	species					488:494	several different host species	465:494	several different host species	465:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	1	11	theme	host	483:486	arg1	species					488:494	several different host species	465:494	several different host species	465:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	9	12	theme	C.	2388:2389	arg1	jejuni					2391:2396	C. jejuni	2388:2396	C. jejuni	2388:2396	We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	6	13	from	diversity	1654:1662	arg1	coli					1700:1703	C. coli	1697:1703	C. coli	1697:1703	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	7	14	theme	causative	1979:1987	arg1	agent					1989:1993	a causative agent	1977:1993	a causative agent of Guillain-Barré syndrome	1977:2020	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	5	15	from	genomes	1514:1520	arg1	absent					1498:1503	absent	1498:1503	absent	1498:1503	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	5	15	from	genomes	1514:1520	arg1	genes					1406:1410	the genes	1402:1410	the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl)	1402:1480	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	2	16	theme	chemical	556:563	arg1	composition					565:575	LOS chemical composition	552:575	LOS chemical composition	552:575	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	1	17	from	diversity	289:297	arg1	Campylobacter					309:321	Campylobacter	309:321	Campylobacter	309:321	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	1	18	theme	classes	389:395	arg1	distribution					363:374	the distribution	359:374	the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species	359:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	1	19	theme	campylobacteriosis	220:237	arg1	pathogenicity					203:215	the pathogenicity	199:215	the pathogenicity of campylobacteriosis	199:237	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	4	20	theme	unexplored	932:941	arg1	heterogeneity					958:970	a completely unexplored LOS structural heterogeneity	919:970	a completely unexplored LOS structural heterogeneity	919:970	This genetic diversity is at the basis of a completely unexplored LOS structural heterogeneity.
27481928	6	21	theme	host	1869:1872	arg1	adaptation					1874:1883	host adaptation	1869:1883	host adaptation	1869:1883	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	7	22	theme	controversial	1955:1967	arg1	role					1969:1972	its controversial role	1951:1972	its controversial role as a causative agent of Guillain-Barré syndrome	1951:2020	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	2	23	theme	first	585:589	arg1	time					591:594	the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity	581:704	the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity	581:704	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	7	24	theme	LOSs	2093:2096	arg1	diversity					2072:2080	the genetic and phenotypic diversity	2045:2080	the genetic and phenotypic diversity of C. coli LOSs	2045:2096	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	5	25	theme	different	1048:1056	arg1	classes					1062:1068	four different LOS classes	1043:1068	four different LOS classes	1043:1068	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	6	26	theme	biosynthesis	1675:1686	arg1	locus					1688:1692	the LOS biosynthesis locus	1667:1692	the LOS biosynthesis locus	1667:1692	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	8	27	theme	information	2223:2233	arg1	paucity					2212:2218	this paucity	2207:2218	this paucity of information	2207:2233	Therefore, we paired C. coli genomic information and LOS chemical composition for the first time to address this paucity of information.
27481928	7	28	theme	C.	2085:2086	arg1	LOSs					2093:2096	C. coli LOSs	2085:2096	C. coli LOSs	2085:2096	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	5	29	theme	GlcN3N	1318:1323	arg1	backbone					1331:1338	the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone	1261:1338	the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates	1261:1609	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	1	30	theme	unrelated	425:433	arg1	isolates					443:450	unrelated C. coli isolates	425:450	unrelated C. coli isolates sampled from several different host species	425:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	9	31	theme	population	2422:2431	arg1	dynamics					2433:2440	population dynamics	2422:2440	population dynamics	2422:2440	We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	7	32	dep	IMPORTANCE	1886:1895	arg1	importance					1909:1918	the importance	1905:1918	the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome	1905:2020	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	5	33	theme	-GlcN	1325:1329	arg1	backbone					1331:1338	the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone	1261:1338	the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates	1261:1609	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	5	34	theme	lipid	1228:1232	arg1	backbone					1236:1243	the lipid A backbone	1224:1243	the lipid A backbone	1224:1243	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	1	35	theme	lipooligosaccharides	168:187	arg1	importance					154:163	the importance	150:163	the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis	150:237	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	1	36	dep	Campylobacter	309:321	arg1	investigated					346:357	investigated	346:357	investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species	346:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	1	36	dep	Campylobacter	309:321	arg1	coli					323:326	Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species	309:494	Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species	309:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	3	37	theme	higher	827:832	arg1	diversity					842:850	higher genetic diversity	827:850	higher genetic diversity than previously thought	827:874	After identifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought.
27481928	5	38	theme	Campylobacter	1135:1147	arg1	jejuni					1149:1154	Campylobacter jejuni	1135:1154	Campylobacter jejuni	1135:1154	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	3	39	dep	diversity	842:850	arg1	thought					868:874	thought	868:874	thought	868:874	After identifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought.
27481928	2	40	theme	genomic	528:534	arg1	information					536:546	C. coli genomic information	520:546	C. coli genomic information	520:546	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	5	41	theme	isolates	1533:1540	arg1	genomes					1514:1520	the genomes	1510:1520	the genomes of various isolates	1510:1540	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	5	42	theme	3-diamino-2,3-dideoxy-d-glucopyranose	1279:1315	arg1	backbone					1331:1338	the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone	1261:1338	the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates	1261:1609	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	0	43	theme	Campylobacter	90:102	arg1	Lipooligosaccharides					109:128	Campylobacter coli Lipooligosaccharides	90:128	Campylobacter coli Lipooligosaccharides	90:128	Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides.
27481928	6	44	theme	genetic	1646:1652	arg1	diversity					1654:1662	the high genetic diversity	1637:1662	the high genetic diversity of the LOS biosynthesis locus in C. coli	1637:1703	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	5	45	theme	spectrometry	978:989	arg1	analysis					991:998	Mass spectrometry analysis	973:998	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes,	973:1069	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	2	46	theme	C.	520:521	arg1	information					536:546	C. coli genomic information	520:546	C. coli genomic information	520:546	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	6	47	theme	species-specific	1720:1735	arg1	features					1748:1755	species-specific phenotypic features	1720:1755	species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation	1720:1883	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	2	48	theme	class	651:655	arg1	diversity					666:674	LOS locus class sequence diversity	641:674	LOS locus class sequence diversity	641:674	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	5	49	located	found	1563:1567	arg1	core					1582:1585	the outer core	1572:1585	the outer core of all C. coli isolates	1572:1609	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	5	49	located	found	1563:1567	arg2	sugar					1553:1557	this rare sugar	1543:1557	this rare sugar	1543:1557	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	0	50	theme	Lipooligosaccharides	109:128	arg1	Diversity					15:23	Large Sequence Diversity	0:23	Large Sequence Diversity within the Biosynthesis Locus	0:53	Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides.
27481928	0	50	theme	Lipooligosaccharides	109:128	arg1	Features					78:85	Common Biochemical Features	59:85	Common Biochemical Features of Campylobacter coli Lipooligosaccharides	59:128	Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides.
27481928	1	51	theme	LOS	379:381	arg1	classes					389:395	LOS locus classes	379:395	LOS locus classes	379:395	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	0	52	dep	Campylobacter	90:102	arg1	coli					104:107	coli	104:107	coli	104:107	Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides.
27481928	9	53	theme	species-specific	2254:2269	arg1	features					2282:2289	two species-specific phenotypic features	2250:2289	two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation	2250:2460	We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	0	54	theme	Large	0:4	arg1	Diversity					15:23	Large Sequence Diversity	0:23	Large Sequence Diversity within the Biosynthesis Locus	0:53	Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides.
27481928	5	55	theme	-GlcN	1188:1192	arg1	disaccharides					1194:1206	2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides	1156:1206	2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides	1156:1206	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	6	56	theme	C.	1811:1812	arg1	jejuni					1814:1819	C. jejuni	1811:1819	C. jejuni	1811:1819	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	2	57	theme	biochemical	680:690	arg1	heterogeneity					692:704	biochemical heterogeneity	680:704	biochemical heterogeneity	680:704	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	5	58	theme	C.	1352:1353	arg1	jejuni					1355:1360	C. jejuni	1352:1360	C. jejuni	1352:1360	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	9	59	theme	C.	2294:2295	arg1	LOS					2302:2304	C. coli LOS	2294:2304	C. coli LOS	2294:2304	We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	5	60	theme	2-Amino-2-deoxy-d-glucose	1156:1180	arg1	disaccharides					1194:1206	2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides	1156:1206	2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides	1156:1206	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	0	61	theme	Common	59:64	arg1	Features					78:85	Common Biochemical Features	59:85	Common Biochemical Features of Campylobacter coli Lipooligosaccharides	59:128	Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides.
27481928	9	62	theme	LOS	2302:2304	arg1	features					2282:2289	two species-specific phenotypic features	2250:2289	two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation	2250:2460	We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	5	63	theme	GlcN	1183:1186	arg1	disaccharides					1194:1206	2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides	1156:1206	2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides	1156:1206	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	6	64	theme	LOS	1768:1770	arg1	features					1748:1755	species-specific phenotypic features	1720:1755	species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation	1720:1883	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	5	65	theme	LOSs	1007:1010	arg1	analysis					991:998	Mass spectrometry analysis	973:998	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes,	973:1069	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	6	66	theme	population	1845:1854	arg1	dynamics					1856:1863	population dynamics	1845:1863	population dynamics	1845:1863	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	5	67	theme	coli	1597:1600	arg1	isolates					1602:1609	all C. coli isolates	1590:1609	all C. coli isolates	1590:1609	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	7	68	theme	genetic	2049:2055	arg1	diversity					2072:2080	the genetic and phenotypic diversity	2045:2080	the genetic and phenotypic diversity of C. coli LOSs	2045:2096	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	5	69	dep	backbone	1331:1338	arg1	contrast					1249:1256	contrast	1249:1256	contrast	1249:1256	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	8	70	theme	C.	2120:2121	arg1	information					2136:2146	C. coli genomic information	2120:2146	C. coli genomic information	2120:2146	Therefore, we paired C. coli genomic information and LOS chemical composition for the first time to address this paucity of information.
27481928	3	71	theme	LOS	735:737	arg1	classes					745:751	three new LOS locus classes	725:751	three new LOS locus classes	725:751	After identifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought.
27481928	9	72	from	differences	2368:2378	arg1	terms					2413:2417	terms	2413:2417	terms of population dynamics	2413:2440	We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	1	73	theme	genetic	266:272	arg1	diversity					289:297	the genetic and phenotypic diversity	262:297	the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species	262:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	6	74	theme	C.	1760:1761	arg1	LOS					1768:1770	C. coli LOS	1760:1770	C. coli LOS	1760:1770	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	8	75	theme	genomic	2128:2134	arg1	information					2136:2146	C. coli genomic information	2120:2146	C. coli genomic information	2120:2146	Therefore, we paired C. coli genomic information and LOS chemical composition for the first time to address this paucity of information.
27481928	1	76	theme	isolates	443:450	arg1	collection					411:420	a large collection	403:420	a large collection of unrelated C. coli isolates sampled from several different host species	403:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	1	77	theme	phenotypic	278:287	arg1	diversity					289:297	the genetic and phenotypic diversity	262:297	the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species	262:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	4	78	theme	genetic	882:888	arg1	diversity					890:898	This genetic diversity	877:898	This genetic diversity	877:898	This genetic diversity is at the basis of a completely unexplored LOS structural heterogeneity.
27481928	2	79	theme	LOS	641:643	arg1	diversity					666:674	LOS locus class sequence diversity	641:674	LOS locus class sequence diversity	641:674	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	8	80	theme	chemical	2156:2163	arg1	composition					2165:2175	LOS chemical composition	2152:2175	LOS chemical composition	2152:2175	Therefore, we paired C. coli genomic information and LOS chemical composition for the first time to address this paucity of information.
27481928	1	81	theme	different	473:481	arg1	species					488:494	several different host species	465:494	several different host species	465:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	7	82	theme	Guillain-Barré	1998:2011	arg1	syndrome					2013:2020	Guillain-Barré syndrome	1998:2020	Guillain-Barré syndrome	1998:2020	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	2	83	theme	LOS	552:554	arg1	composition					565:575	LOS chemical composition	552:575	LOS chemical composition	552:575	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	5	84	link	β-1'-6-linked	1265:1277	arg1	backbone					1331:1338	the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone	1261:1338	the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates	1261:1609	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	8	85	theme	first	2185:2189	arg1	time					2191:2194	the first time to address this paucity of information	2181:2233	the first time to address this paucity of information	2181:2233	Therefore, we paired C. coli genomic information and LOS chemical composition for the first time to address this paucity of information.
27481928	6	86	theme	adaptation	1874:1883	arg1	terms					1836:1840	terms	1836:1840	terms of population dynamics and host adaptation	1836:1883	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	4	87	theme	structural	947:956	arg1	heterogeneity					958:970	a completely unexplored LOS structural heterogeneity	919:970	a completely unexplored LOS structural heterogeneity	919:970	This genetic diversity is at the basis of a completely unexplored LOS structural heterogeneity.
27481928	1	88	theme	large	405:409	arg1	collection					411:420	a large collection	403:420	a large collection of unrelated C. coli isolates sampled from several different host species	403:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	6	89	theme	locus	1688:1692	arg1	diversity					1654:1662	the high genetic diversity	1637:1662	the high genetic diversity of the LOS biosynthesis locus in C. coli	1637:1703	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	3	90	theme	isolates	774:781	arg1	isolates					774:781	the 144 isolates	766:781	the 144 isolates tested	766:788	After identifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought.
27481928	3	90	theme	isolates	774:781	arg1	%					761:761	only 85%	754:761	only 85% of the 144 isolates tested	754:788	After identifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought.
27481928	5	91	from	backbone	1236:1243	arg1	present					1213:1219	present	1213:1219	present	1213:1219	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	5	92	theme	LOS	1058:1060	arg1	classes					1062:1068	four different LOS classes	1043:1068	four different LOS classes	1043:1068	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	6	93	theme	LOS	1671:1673	arg1	locus					1688:1692	the LOS biosynthesis locus	1667:1692	the LOS biosynthesis locus	1667:1692	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	7	94	theme	coli	2088:2091	arg1	LOSs					2093:2096	C. coli LOSs	2085:2096	C. coli LOSs	2085:2096	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	0	95	theme	Biochemical	66:76	arg1	Features					78:85	Common Biochemical Features	59:85	Common Biochemical Features of Campylobacter coli Lipooligosaccharides	59:128	Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides.
27481928	1	96	theme	C.	435:436	arg1	isolates					443:450	unrelated C. coli isolates	425:450	unrelated C. coli isolates sampled from several different host species	425:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	9	97	theme	dynamics	2433:2440	arg1	terms					2413:2417	terms	2413:2417	terms of population dynamics	2413:2440	We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	6	98	theme	coli	1763:1766	arg1	LOS					1768:1770	C. coli LOS	1760:1770	C. coli LOS	1760:1770	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	2	99	theme	coli	523:526	arg1	information					536:546	C. coli genomic information	520:546	C. coli genomic information	520:546	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	9	100	theme	host	2446:2449	arg1	adaptation					2451:2460	host adaptation	2446:2460	host adaptation	2446:2460	We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	6	101	from	differences	1791:1801	arg1	terms					1836:1840	terms	1836:1840	terms of population dynamics and host adaptation	1836:1883	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	5	102	theme	various	1525:1531	arg1	isolates					1533:1540	various isolates	1525:1540	various isolates	1525:1540	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	7	103	theme	phenotypic	2061:2070	arg1	diversity					2072:2080	the genetic and phenotypic diversity	2045:2080	the genetic and phenotypic diversity of C. coli LOSs	2045:2096	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	5	104	theme	β-1'-6-linked	1265:1277	arg1	backbone					1331:1338	the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone	1261:1338	the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates	1261:1609	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	3	105	theme	genetic	834:840	arg1	diversity					842:850	higher genetic diversity	827:850	higher genetic diversity than previously thought	827:874	After identifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought.
27481928	7	106	theme	human	1934:1938	arg1	health					1940:1945	human health	1934:1945	human health	1934:1945	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	5	107	theme	Mass	973:976	arg1	analysis					991:998	Mass spectrometry analysis	973:998	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes,	973:1069	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	4	108	theme	LOS	943:945	arg1	heterogeneity					958:970	a completely unexplored LOS structural heterogeneity	919:970	a completely unexplored LOS structural heterogeneity	919:970	This genetic diversity is at the basis of a completely unexplored LOS structural heterogeneity.
27481928	6	109	theme	phenotypic	1737:1746	arg1	features					1748:1755	species-specific phenotypic features	1720:1755	species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation	1720:1883	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	2	110	theme	sequence	657:664	arg1	diversity					666:674	LOS locus class sequence diversity	641:674	LOS locus class sequence diversity	641:674	Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity.
27481928	7	111	theme	coli	1926:1929	arg1	importance					1909:1918	the importance	1905:1918	the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome	1905:2020	IMPORTANCE Despite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs.
27481928	6	112	theme	high	1641:1644	arg1	diversity					1654:1662	the high genetic diversity	1637:1662	the high genetic diversity of the LOS biosynthesis locus in C. coli	1637:1703	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	5	113	located	present	1213:1219	arg2	disaccharides					1194:1206	2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides	1156:1206	2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides	1156:1206	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	5	113	located	present	1213:1219	arg1	backbone					1236:1243	the lipid A backbone	1224:1243	the lipid A backbone	1224:1243	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	4	114	theme	heterogeneity	958:970	arg1	basis					910:914	the basis	906:914	the basis of a completely unexplored LOS structural heterogeneity	906:970	This genetic diversity is at the basis of a completely unexplored LOS structural heterogeneity.
27481928	0	115	theme	Sequence	6:13	arg1	Diversity					15:23	Large Sequence Diversity	0:23	Large Sequence Diversity within the Biosynthesis Locus	0:53	Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides.
27481928	1	116	theme	locus	383:387	arg1	classes					389:395	LOS locus classes	379:395	LOS locus classes	379:395	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	1	117	from	importance	154:163	arg1	pathogenicity					203:215	the pathogenicity	199:215	the pathogenicity of campylobacteriosis	199:237	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	9	118	theme	phenotypic	2271:2280	arg1	features					2282:2289	two species-specific phenotypic features	2250:2289	two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation	2250:2460	We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	1	119	theme	LOS	302:304	arg1	diversity					289:297	the genetic and phenotypic diversity	262:297	the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species	262:494	UNLABELLED Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species.
27481928	5	120	dep	that	1127:1130	arg1	present					1213:1219	present	1213:1219	present	1213:1219	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	0	121	theme	Biosynthesis	36:47	arg1	Locus					49:53	the Biosynthesis Locus	32:53	the Biosynthesis Locus	32:53	Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides.
27481928	5	122	theme	isolates	1020:1027	arg1	LOSs					1007:1010	the LOSs	1003:1010	the LOSs of nine isolates, representing four different LOS classes,	1003:1069	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	5	123	theme	outer	1576:1580	arg1	core					1582:1585	the outer core	1572:1585	the outer core of all C. coli isolates	1572:1609	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	9	124	theme	coli	2297:2300	arg1	LOS					2302:2304	C. coli LOS	2294:2304	C. coli LOS	2294:2304	We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	5	125	dep	C.	1110:1111	arg1	LOS					1118:1120	C. coli LOS	1110:1120	C. coli LOS	1110:1120	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	5	126	theme	rare	1548:1551	arg1	sugar					1553:1557	this rare sugar	1543:1557	this rare sugar	1543:1557	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	6	127	theme	dynamics	1856:1863	arg1	terms					1836:1840	terms	1836:1840	terms of population dynamics and host adaptation	1836:1883	Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
27481928	8	128	theme	coli	2123:2126	arg1	information					2136:2146	C. coli genomic information	2120:2146	C. coli genomic information	2120:2146	Therefore, we paired C. coli genomic information and LOS chemical composition for the first time to address this paucity of information.
27481928	5	129	theme	isolates	1602:1609	arg1	core					1582:1585	the outer core	1572:1585	the outer core of all C. coli isolates	1572:1609	Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni 2-Amino-2-deoxy-d-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the β-1'-6-linked 3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-d-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates.
27481928	3	130	theme	new	731:733	arg1	classes					745:751	three new LOS locus classes	725:751	three new LOS locus classes	725:751	After identifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought.
25267245	3	0	theme	day	648:650	arg1	length					652:657	long day length	643:657	long day length	643:657	At the agronomic level, oats exhibit optimal growth in regions of moderate temperature and long day length.
25267245	8	1	theme	wide	1734:1737	arg1	r					1739:1739	r	1739:1739	r	1739:1739	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	8	1	theme	wide	1734:1737	arg1	range					1742:1746	a wide(r) range	1732:1746	a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition	1732:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	2	2	theme	whole	324:328	arg1	grain					330:334	whole grain	324:334	whole grain	324:334	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	8	3	theme	varieties	1589:1597	arg1	generation					1557:1566	the generation	1553:1566	the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition	1553:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	1	4	theme	nutrients	219:227	arg1	many					207:210	many	207:210	many	207:210	Oats are undervalued in comparison with wheat, rice and barley, despite their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence.
25267245	1	4	theme	nutrients	219:227	arg1	nutrients					219:227	the nutrients	215:227	the nutrients required for health and a reduced risk of degenerative disease incidence	215:300	Oats are undervalued in comparison with wheat, rice and barley, despite their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence.
25267245	2	5	theme	health	443:448	arg1	claim					450:454	an approved health claim	431:454	an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d	431:549	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	3	6	theme	temperature	627:637	arg1	regions					607:613	regions	607:613	regions of moderate temperature and long day length	607:657	At the agronomic level, oats exhibit optimal growth in regions of moderate temperature and long day length.
25267245	7	7	theme	analytical	1404:1413	arg1	approaches					1415:1424	the allied next-level analytical approaches	1382:1424	the allied next-level analytical approaches	1382:1424	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	5	8	dep	content	822:828	arg1	the					818:820	the	818:820	the	818:820	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	5	9	theme	different	989:997	arg1	practices					1009:1017	different agronomic practices	989:1017	different agronomic practices	989:1017	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	8	10	with	products	1760:1767	arg1	ultimate					1778:1785	ultimate	1778:1785	ultimate	1778:1785	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	2	11	theme	approved	434:441	arg1	claim					450:454	an approved health claim	431:454	an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d	431:549	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	6	12	theme	plant	1092:1096	arg1	genetics					1098:1105	modern plant genetics	1085:1105	modern plant genetics	1085:1105	The advances in modern plant genetics, developed in sister cereals such as wheat, rice and barley, mean that oat development and exploitation should see an acceleration in the coming decade as they are adopted and applied.
25267245	7	13	theme	genome	1334:1339	arg1	sequencing					1341:1350	genome sequencing	1334:1350	genome sequencing	1334:1350	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	8	14	dep	new	1571:1573	arg1	tailored					1576:1583	tailored	1576:1583	tailored	1576:1583	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	6	15	theme	modern	1085:1090	arg1	genetics					1098:1105	modern plant genetics	1085:1105	modern plant genetics	1085:1105	The advances in modern plant genetics, developed in sister cereals such as wheat, rice and barley, mean that oat development and exploitation should see an acceleration in the coming decade as they are adopted and applied.
25267245	8	16	contain	contain	1635:1641	arg2	proportion					1653:1662	a greater proportion	1643:1662	a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition	1643:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	8	16	contain	contain	1635:1641	arg1	varieties					1589:1597	new, tailored oat varieties	1571:1597	new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition	1571:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	4	17	theme	wet	691:693	arg1	weather					695:701	wet weather	691:701	wet weather	691:701	In addition, they can tolerate wet weather and acidic soils more effectively than other cereals, such as wheat.
25267245	7	18	theme	metabolomics	1473:1484	arg1	sequencing					1367:1376	sequencing	1367:1376	sequencing	1367:1376	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	7	18	theme	metabolomics	1473:1484	arg1	approaches					1415:1424	the allied next-level analytical approaches	1382:1424	the allied next-level analytical approaches	1382:1424	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	2	19	theme	cholesterol	472:482	arg1	levels					484:489	blood cholesterol levels	466:489	blood cholesterol levels	466:489	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	6	20	theme	mean	1168:1171	arg1	advances					1073:1080	The advances	1069:1080	The advances	1069:1080	The advances in modern plant genetics, developed in sister cereals such as wheat, rice and barley, mean that oat development and exploitation should see an acceleration in the coming decade as they are adopted and applied.
25267245	5	21	from	diversity	805:813	arg1	composition					834:844	composition	834:844	composition	834:844	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	5	21	from	diversity	805:813	arg1	content					822:828	content	822:828	content	822:828	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	4	22	theme	acidic	707:712	arg1	soils					714:718	acidic soils	707:718	acidic soils	707:718	In addition, they can tolerate wet weather and acidic soils more effectively than other cereals, such as wheat.
25267245	2	23	theme	associated	354:363	arg1	products					365:372	their associated products	348:372	their associated products	348:372	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	2	24	theme	blood	466:470	arg1	levels					484:489	blood cholesterol levels	466:489	blood cholesterol levels	466:489	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	3	25	theme	length	652:657	arg1	regions					607:613	regions	607:613	regions of moderate temperature and long day length	607:657	At the agronomic level, oats exhibit optimal growth in regions of moderate temperature and long day length.
25267245	1	26	with	comparison	120:129	arg1	wheat					136:140	wheat	136:140	wheat	136:140	Oats are undervalued in comparison with wheat, rice and barley, despite their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence.
25267245	1	26	with	comparison	120:129	arg1	barley					152:157	barley	152:157	barley	152:157	Oats are undervalued in comparison with wheat, rice and barley, despite their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence.
25267245	1	26	with	comparison	120:129	arg1	rice					143:146	rice	143:146	rice	143:146	Oats are undervalued in comparison with wheat, rice and barley, despite their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence.
25267245	2	27	theme	complex	399:405	arg1	polysaccharide					407:420	a complex polysaccharide	397:420	a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d	397:549	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	2	27	theme	complex	399:405	arg1	β-glucan					387:394	β-glucan	387:394	β-glucan	387:394	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	5	28	dep	amenable	962:969	arg1	enhanced					977:984	enhanced	977:984	to be enhanced by different agronomic practices as well as are susceptible to climatic variation	971:1066	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	5	28	dep	amenable	962:969	arg1	susceptible					1034:1044	susceptible	1034:1044	susceptible	1034:1044	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	0	29	theme	agriculture	4:14	arg1	targets					42:48	Oat agriculture, cultivation and breeding targets	0:48	Oat agriculture, cultivation and breeding targets: implications for human nutrition and health.	0:94	Oat agriculture, cultivation and breeding targets: implications for human nutrition and health.
25267245	0	30	theme	human	68:72	arg1	nutrition					74:82	human nutrition	68:82	human nutrition	68:82	Oat agriculture, cultivation and breeding targets: implications for human nutrition and health.
25267245	2	31	contain	has	427:429	arg1	polysaccharide					407:420	a complex polysaccharide	397:420	a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d	397:549	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	2	31	contain	has	427:429	arg2	claim					450:454	an approved health claim	431:454	an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d	431:549	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	2	31	contain	has	427:429	arg1	β-glucan					387:394	β-glucan	387:394	β-glucan	387:394	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	5	32	theme	nutrients	849:857	arg1	composition					834:844	composition	834:844	composition	834:844	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	5	32	theme	nutrients	849:857	arg1	content					822:828	content	822:828	content	822:828	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	0	33	theme	Oat	0:2	arg1	agriculture					4:14	Oat agriculture	0:14	Oat agriculture	0:14	Oat agriculture, cultivation and breeding targets: implications for human nutrition and health.
25267245	8	34	theme	benefits	1806:1813	arg1	ultimate					1778:1785	ultimate	1778:1785	ultimate	1778:1785	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	7	35	theme	next-level	1393:1402	arg1	approaches					1415:1424	the allied next-level analytical approaches	1382:1424	the allied next-level analytical approaches	1382:1424	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	8	36	theme	associated	1795:1804	arg1	benefits					1806:1813	associated benefits	1795:1813	associated benefits to human health and nutrition	1795:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	6	37	from	advances	1073:1080	arg1	genetics					1098:1105	modern plant genetics	1085:1105	modern plant genetics	1085:1105	The advances in modern plant genetics, developed in sister cereals such as wheat, rice and barley, mean that oat development and exploitation should see an acceleration in the coming decade as they are adopted and applied.
25267245	7	38	theme	profiling	1459:1467	arg1	sequencing					1367:1376	sequencing	1367:1376	sequencing	1367:1376	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	7	38	theme	profiling	1459:1467	arg1	approaches					1415:1424	the allied next-level analytical approaches	1382:1424	the allied next-level analytical approaches	1382:1424	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	0	39	theme	cultivation	17:27	arg1	targets					42:48	Oat agriculture, cultivation and breeding targets	0:48	Oat agriculture, cultivation and breeding targets: implications for human nutrition and health.	0:94	Oat agriculture, cultivation and breeding targets: implications for human nutrition and health.
25267245	6	40	from	acceleration	1225:1236	arg1	decade					1252:1257	the coming decade	1241:1257	the coming decade	1241:1257	The advances in modern plant genetics, developed in sister cereals such as wheat, rice and barley, mean that oat development and exploitation should see an acceleration in the coming decade as they are adopted and applied.
25267245	6	41	theme	coming	1245:1250	arg1	decade					1252:1257	the coming decade	1241:1257	the coming decade	1241:1257	The advances in modern plant genetics, developed in sister cereals such as wheat, rice and barley, mean that oat development and exploitation should see an acceleration in the coming decade as they are adopted and applied.
25267245	8	42	theme	consumer	1751:1758	arg1	products					1760:1767	consumer products	1751:1767	consumer products with the ultimate hope of associated benefits to human health and nutrition	1751:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	5	43	theme	health-beneficial	863:879	arg1	components					881:890	health-beneficial components	863:890	health-beneficial components	863:890	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	8	44	theme	greater	1645:1651	arg1	proportion					1653:1662	a greater proportion	1643:1662	a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition	1643:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	1	45	theme	reduced	255:261	arg1	risk					263:266	a reduced risk	253:266	a reduced risk of degenerative disease incidence	253:300	Oats are undervalued in comparison with wheat, rice and barley, despite their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence.
25267245	5	46	theme	available	903:911	arg1	germplasm					933:941	the available wild and cultivated germplasm	899:941	the available wild and cultivated germplasm	899:941	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	3	47	theme	optimal	589:595	arg1	growth					597:602	optimal growth	589:602	optimal growth in regions of moderate temperature and long day length	589:657	At the agronomic level, oats exhibit optimal growth in regions of moderate temperature and long day length.
25267245	5	48	theme	components	881:890	arg1	composition					834:844	composition	834:844	composition	834:844	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	5	48	theme	components	881:890	arg1	content					822:828	content	822:828	content	822:828	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	8	49	theme	health-beneficial	1667:1683	arg1	components					1685:1694	health-beneficial components	1667:1694	health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition	1667:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	1	50	theme	unique	174:179	arg1	composition					181:191	their unique composition	168:191	their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence	168:300	Oats are undervalued in comparison with wheat, rice and barley, despite their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence.
25267245	0	51	theme	breeding	33:40	arg1	targets					42:48	Oat agriculture, cultivation and breeding targets	0:48	Oat agriculture, cultivation and breeding targets: implications for human nutrition and health.	0:94	Oat agriculture, cultivation and breeding targets: implications for human nutrition and health.
25267245	7	52	theme	transcriptome	1445:1457	arg1	profiling					1459:1467	transcriptome profiling	1445:1467	transcriptome profiling	1445:1467	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	5	53	theme	agronomic	999:1007	arg1	practices					1009:1017	different agronomic practices	989:1017	different agronomic practices	989:1017	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	7	54	theme	sequencing	1433:1442	arg1	sequencing					1367:1376	sequencing	1367:1376	sequencing	1367:1376	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	7	54	theme	sequencing	1433:1442	arg1	approaches					1415:1424	the allied next-level analytical approaches	1382:1424	the allied next-level analytical approaches	1382:1424	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	0	55	dep	targets	42:48	arg1	implications					51:62	implications	51:62	Oat agriculture, cultivation and breeding targets: implications for human nutrition and health.	0:94	Oat agriculture, cultivation and breeding targets: implications for human nutrition and health.
25267245	3	56	from	growth	597:602	arg1	regions					607:613	regions	607:613	regions of moderate temperature and long day length	607:657	At the agronomic level, oats exhibit optimal growth in regions of moderate temperature and long day length.
25267245	1	57	theme	degenerative	271:282	arg1	incidence					292:300	degenerative disease incidence	271:300	degenerative disease incidence	271:300	Oats are undervalued in comparison with wheat, rice and barley, despite their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence.
25267245	8	58	dep	collation	1491:1499	arg1	The					1487:1489	The	1487:1489	The	1487:1489	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	8	59	theme	components	1685:1694	arg1	proportion					1653:1662	a greater proportion	1643:1662	a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition	1643:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	8	60	theme	products	1760:1767	arg1	r					1739:1739	r	1739:1739	r	1739:1739	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	8	60	theme	products	1760:1767	arg1	range					1742:1746	a wide(r) range	1732:1746	a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition	1732:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	1	61	theme	disease	284:290	arg1	incidence					292:300	degenerative disease incidence	271:300	degenerative disease incidence	271:300	Oats are undervalued in comparison with wheat, rice and barley, despite their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence.
25267245	7	62	theme	allied	1386:1391	arg1	approaches					1415:1424	the allied next-level analytical approaches	1382:1424	the allied next-level analytical approaches	1382:1424	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	7	63	theme	RNA	1429:1431	arg1	sequencing					1433:1442	RNA sequencing	1429:1442	RNA sequencing	1429:1442	These advances include approaches such as genome sequencing, genotyping by sequencing and the allied next-level analytical approaches of RNA sequencing, transcriptome profiling and metabolomics.
25267245	6	64	theme	oat	1178:1180	arg1	development					1182:1192	oat development	1178:1192	oat development	1178:1192	The advances in modern plant genetics, developed in sister cereals such as wheat, rice and barley, mean that oat development and exploitation should see an acceleration in the coming decade as they are adopted and applied.
25267245	1	65	theme	incidence	292:300	arg1	risk					263:266	a reduced risk	253:266	a reduced risk of degenerative disease incidence	253:300	Oats are undervalued in comparison with wheat, rice and barley, despite their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence.
25267245	1	65	theme	incidence	292:300	arg1	health					242:247	health	242:247	health	242:247	Oats are undervalued in comparison with wheat, rice and barley, despite their unique composition that includes many of the nutrients required for health and a reduced risk of degenerative disease incidence.
25267245	2	66	theme	incidence	518:526	arg1	risk					506:509	the risk	502:509	the risk of CHD incidence	502:526	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	3	67	theme	moderate	618:625	arg1	temperature					627:637	moderate temperature	618:637	moderate temperature	618:637	At the agronomic level, oats exhibit optimal growth in regions of moderate temperature and long day length.
25267245	8	68	theme	human	1818:1822	arg1	health					1824:1829	human health	1818:1829	human health	1818:1829	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	4	69	theme	other	742:746	arg1	cereals					748:754	other cereals	742:754	other cereals	742:754	In addition, they can tolerate wet weather and acidic soils more effectively than other cereals, such as wheat.
25267245	4	69	theme	other	742:746	arg1	wheat					765:769	wheat	765:769	wheat	765:769	In addition, they can tolerate wet weather and acidic soils more effectively than other cereals, such as wheat.
25267245	8	70	theme	new	1571:1573	arg1	varieties					1589:1597	new, tailored oat varieties	1571:1597	new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition	1571:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	8	71	theme	approaches	1527:1536	arg1	coordination					1505:1516	coordination	1505:1516	coordination	1505:1516	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	8	71	theme	approaches	1527:1536	arg1	collation					1491:1499	collation	1491:1499	collation	1491:1499	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	2	72	theme	CHD	514:516	arg1	incidence					518:526	CHD incidence	514:526	CHD incidence	514:526	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	2	73	contain	contain	379:385	arg2	β-glucan					387:394	β-glucan	387:394	β-glucan	387:394	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	2	73	contain	contain	379:385	arg1	some					340:343	some	340:343	some	340:343	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	2	73	contain	contain	379:385	arg1	oats					316:319	oats	316:319	oats as whole grain	316:334	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	2	73	contain	contain	379:385	arg2	polysaccharide					407:420	a complex polysaccharide	397:420	a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d	397:549	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	2	73	contain	contain	379:385	arg1	products					365:372	their associated products	348:372	their associated products	348:372	Furthermore, oats as whole grain and some of their associated products also contain β-glucan, a complex polysaccharide that has an approved health claim to reduce blood cholesterol levels and reduce the risk of CHD incidence if consumed at ≥ 3 g/d.
25267245	5	74	theme	cultivated	922:931	arg1	germplasm					933:941	the available wild and cultivated germplasm	899:941	the available wild and cultivated germplasm	899:941	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	8	75	theme	oat	1585:1587	arg1	varieties					1589:1597	new, tailored oat varieties	1571:1597	new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition	1571:1843	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
25267245	6	76	theme	sister	1121:1126	arg1	cereals					1128:1134	sister cereals	1121:1134	sister cereals such as wheat, rice and barley	1121:1165	The advances in modern plant genetics, developed in sister cereals such as wheat, rice and barley, mean that oat development and exploitation should see an acceleration in the coming decade as they are adopted and applied.
25267245	6	76	theme	sister	1121:1126	arg1	wheat					1144:1148	wheat	1144:1148	wheat	1144:1148	The advances in modern plant genetics, developed in sister cereals such as wheat, rice and barley, mean that oat development and exploitation should see an acceleration in the coming decade as they are adopted and applied.
25267245	6	76	theme	sister	1121:1126	arg1	barley					1160:1165	barley	1160:1165	barley	1160:1165	The advances in modern plant genetics, developed in sister cereals such as wheat, rice and barley, mean that oat development and exploitation should see an acceleration in the coming decade as they are adopted and applied.
25267245	6	76	theme	sister	1121:1126	arg1	rice					1151:1154	rice	1151:1154	rice	1151:1154	The advances in modern plant genetics, developed in sister cereals such as wheat, rice and barley, mean that oat development and exploitation should see an acceleration in the coming decade as they are adopted and applied.
25267245	5	77	theme	climatic	1049:1056	arg1	variation					1058:1066	climatic variation	1049:1066	climatic variation	1049:1066	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	3	78	theme	long	643:646	arg1	length					652:657	long day length	643:657	long day length	643:657	At the agronomic level, oats exhibit optimal growth in regions of moderate temperature and long day length.
25267245	3	79	theme	agronomic	559:567	arg1	level					569:573	the agronomic level	555:573	the agronomic level	555:573	At the agronomic level, oats exhibit optimal growth in regions of moderate temperature and long day length.
25267245	5	80	theme	wild	913:916	arg1	germplasm					933:941	the available wild and cultivated germplasm	899:941	the available wild and cultivated germplasm	899:941	Studies have shown that there is diversity in the content and composition of nutrients and health-beneficial components within the available wild and cultivated germplasm and that these are amenable to be enhanced by different agronomic practices as well as are susceptible to climatic variation.
25267245	8	81	dep	ultimate	1778:1785	arg1	hope					1787:1790	hope	1787:1790	hope	1787:1790	The collation and coordination of these approaches should lead to the generation of new, tailored oat varieties that are nutritionally enhanced and contain a greater proportion of health-beneficial components that can be translated through into a wide(r) range of consumer products with the ultimate hope of associated benefits to human health and nutrition.
26187194	5	0	theme	milieu	926:931	arg1	pH					920:921	pH	920:921	pH of milieu, amount of adsorbent, and contact time	920:970	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
26187194	6	1	theme	isotherm	1092:1099	arg1	constants					1101:1109	adsorption equilibrium and isotherm constants	1065:1109	constants	1101:1109	The Freundlich and Langmuir adsorption models were used for the mathematical description of adsorption equilibrium and isotherm constants.
26187194	9	2	from	solution	1432:1439	arg1	removal					1396:1402	the removal	1392:1402	the removal of Pb(II) ions from aqueous solution	1392:1439	The study suggests that the GO/Ch/FeOOH is a promising nano adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	6	3	theme	mathematical	1037:1048	arg1	description					1050:1060	the mathematical description	1033:1060	the mathematical description of adsorption equilibrium and isotherm constants	1033:1109	The Freundlich and Langmuir adsorption models were used for the mathematical description of adsorption equilibrium and isotherm constants.
26187194	6	4	theme	adsorption	1065:1074	arg1	equilibrium					1076:1086	adsorption equilibrium and isotherm constants	1065:1109	equilibrium	1076:1086	The Freundlich and Langmuir adsorption models were used for the mathematical description of adsorption equilibrium and isotherm constants.
26187194	6	5	used	used	1024:1027	arg2	models					1012:1017	The Freundlich and Langmuir adsorption models	973:1017	The Freundlich and Langmuir adsorption models	973:1017	The Freundlich and Langmuir adsorption models were used for the mathematical description of adsorption equilibrium and isotherm constants.
26187194	4	6	theme	Pb	784:785	arg1	ions					791:794	the Pb(II) ions	780:794	the Pb(II) ions	780:794	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	7	theme	surface	620:626	arg1	property					628:635	the surface property	616:635	the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan	616:716	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	6	8	theme	equilibrium	1076:1086	arg1	description					1050:1060	the mathematical description	1033:1060	the mathematical description of adsorption equilibrium and isotherm constants	1033:1109	The Freundlich and Langmuir adsorption models were used for the mathematical description of adsorption equilibrium and isotherm constants.
26187194	4	9	theme	abundant	660:667	arg1	amino					669:673	the abundant amino and hydroxyl functional groups	656:704	amino	669:673	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	9	theme	abundant	660:667	arg1	oxide					649:653	graphene oxide	640:653	graphene oxide	640:653	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	8	10	theme	Freundlich	1300:1309	arg1	model					1311:1315	Freundlich model	1300:1315	Freundlich model	1300:1315	However, Langmuir model showed higher correlation coefficient (R(2)) than Freundlich model.
26187194	4	11	theme	chitosan	709:716	arg1	amino					669:673	the abundant amino and hydroxyl functional groups	656:704	amino	669:673	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	11	theme	chitosan	709:716	arg1	chitosan					709:716	chitosan	709:716	chitosan	709:716	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	11	theme	chitosan	709:716	arg1	oxide					649:653	graphene oxide	640:653	graphene oxide	640:653	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	11	theme	chitosan	709:716	arg1	groups					699:704	the abundant amino and hydroxyl functional groups	656:704	groups	699:704	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	9	12	theme	Pb	1407:1408	arg1	ions					1414:1417	Pb(II) ions	1407:1417	Pb(II) ions	1407:1417	The study suggests that the GO/Ch/FeOOH is a promising nano adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	9	13	theme	adsorbent	1378:1386	arg1	GO/Ch/FeOOH					1346:1356	the GO/Ch/FeOOH	1342:1356	the GO/Ch/FeOOH	1342:1356	The study suggests that the GO/Ch/FeOOH is a promising nano adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	9	13	theme	adsorbent	1378:1386	arg1	nano					1373:1376	a promising nano	1361:1376	a promising nano adsorbent for the removal of Pb(II) ions from aqueous solution	1361:1439	The study suggests that the GO/Ch/FeOOH is a promising nano adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	7	14	from	description	1148:1158	arg1	range					1211:1215	the concentration range	1193:1215	the concentration range studied	1193:1223	Both models were applicable for the description of Pb(II) adsorption isotherm in the concentration range studied.
26187194	2	15	theme	XRD	339:341	arg1	techniques					352:361	FT-IR, XRD, and SEM techniques	332:361	FT-IR, XRD, and SEM techniques	332:361	The nanocomposite was characterized by FT-IR, XRD, and SEM techniques.
26187194	6	16	theme	Langmuir	992:999	arg1	adsorption					1001:1010	Langmuir adsorption	992:1010	Langmuir adsorption	992:1010	The Freundlich and Langmuir adsorption models were used for the mathematical description of adsorption equilibrium and isotherm constants.
26187194	0	17	theme	aqueous	90:96	arg1	solution					98:105	aqueous solution	90:105	aqueous solution	90:105	Preparation of graphene oxide/chitosan/FeOOH nanocomposite for the removal of Pb(II) from aqueous solution.
26187194	9	18	theme	aqueous	1424:1430	arg1	solution					1432:1439	aqueous solution	1424:1439	aqueous solution	1424:1439	The study suggests that the GO/Ch/FeOOH is a promising nano adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	4	19	theme	functional	688:697	arg1	chitosan					709:716	chitosan	709:716	chitosan	709:716	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	19	theme	functional	688:697	arg1	oxide					649:653	graphene oxide	640:653	graphene oxide	640:653	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	19	theme	functional	688:697	arg1	groups					699:704	the abundant amino and hydroxyl functional groups	656:704	groups	699:704	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	5	20	theme	batch	821:825	arg1	experiments					838:848	The batch adsorption experiments	817:848	The batch adsorption experiments	817:848	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
26187194	7	21	theme	concentration	1197:1209	arg1	range					1211:1215	the concentration range	1193:1215	the concentration range studied	1193:1223	Both models were applicable for the description of Pb(II) adsorption isotherm in the concentration range studied.
26187194	3	22	theme	important	372:380	arg1	parameters					382:391	Several important parameters	364:391	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg)	364:559	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	5	23	theme	contact	959:965	arg1	time					967:970	contact time	959:970	contact time	959:970	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
26187194	4	24	theme	hydroxyl	679:686	arg1	chitosan					709:716	chitosan	709:716	chitosan	709:716	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	24	theme	hydroxyl	679:686	arg1	oxide					649:653	graphene oxide	640:653	graphene oxide	640:653	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	24	theme	hydroxyl	679:686	arg1	groups					699:704	the abundant amino and hydroxyl functional groups	656:704	groups	699:704	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	7	25	theme	Pb	1163:1164	arg1	isotherm					1181:1188	Pb(II) adsorption isotherm	1163:1188	Pb(II) adsorption isotherm	1163:1188	Both models were applicable for the description of Pb(II) adsorption isotherm in the concentration range studied.
26187194	0	26	theme	oxide/chitosan/FeOOH	24:43	arg1	Preparation					0:10	Preparation	0:10	Preparation of graphene oxide/chitosan/FeOOH	0:43	Preparation of graphene oxide/chitosan/FeOOH nanocomposite for the removal of Pb(II) from aqueous solution.
26187194	1	27	theme	Pb	258:259	arg1	ions					265:268	Pb(II) ions	258:268	Pb(II) ions	258:268	In the present study, a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite was prepared and used as an adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	4	28	theme	versatile	755:763	arg1	adsorption					765:774	adequate and versatile adsorption	742:774	adequate and versatile adsorption	742:774	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	9	29	theme	promising	1363:1371	arg1	GO/Ch/FeOOH					1346:1356	the GO/Ch/FeOOH	1342:1356	the GO/Ch/FeOOH	1342:1356	The study suggests that the GO/Ch/FeOOH is a promising nano adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	9	29	theme	promising	1363:1371	arg1	nano					1373:1376	a promising nano	1361:1376	a promising nano adsorbent for the removal of Pb(II) ions from aqueous solution	1361:1439	The study suggests that the GO/Ch/FeOOH is a promising nano adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	3	30	theme	Pb	423:424	arg1	pH					443:444	pH	443:444	pH (3-7)	443:450	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	30	theme	Pb	423:424	arg1	time					514:517	contact time	506:517	contact time (10-70 min)	506:529	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	30	theme	Pb	423:424	arg1	mass					544:547	sorbent mass	536:547	sorbent mass (10-100 mg)	536:559	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	30	theme	Pb	423:424	arg1	ions					430:433	Pb(II) ions	423:433	Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg)	423:559	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	30	theme	Pb	423:424	arg1	temperature					453:463	temperature	453:463	temperature (25-80 °C)	453:474	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	30	theme	Pb	423:424	arg1	speed					485:489	shaking speed	477:489	shaking speed (150-800 rpm)	477:503	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	0	31	theme	graphene	15:22	arg1	oxide/chitosan/FeOOH					24:43	graphene oxide/chitosan/FeOOH	15:43	graphene oxide/chitosan/FeOOH	15:43	Preparation of graphene oxide/chitosan/FeOOH nanocomposite for the removal of Pb(II) from aqueous solution.
26187194	8	32	theme	Langmuir	1235:1242	arg1	model					1244:1248	Langmuir model	1235:1248	Langmuir model	1235:1248	However, Langmuir model showed higher correlation coefficient (R(2)) than Freundlich model.
26187194	7	33	theme	adsorption	1170:1179	arg1	isotherm					1181:1188	Pb(II) adsorption isotherm	1163:1188	Pb(II) adsorption isotherm	1163:1188	Both models were applicable for the description of Pb(II) adsorption isotherm in the concentration range studied.
26187194	6	34	theme	Freundlich	977:986	arg1	models					1012:1017	The Freundlich and Langmuir adsorption models	973:1017	The Freundlich and Langmuir adsorption models	973:1017	The Freundlich and Langmuir adsorption models were used for the mathematical description of adsorption equilibrium and isotherm constants.
26187194	3	35	theme	Several	364:370	arg1	parameters					382:391	Several important parameters	364:391	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg)	364:559	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	8	36	theme	higher	1257:1262	arg1	coefficient					1276:1286	higher correlation coefficient	1257:1286	higher correlation coefficient (R(2))	1257:1293	However, Langmuir model showed higher correlation coefficient (R(2)) than Freundlich model.
26187194	8	36	theme	higher	1257:1262	arg1	R					1289:1289	R(2)	1289:1292	R(2)	1289:1292	However, Langmuir model showed higher correlation coefficient (R(2)) than Freundlich model.
26187194	4	37	theme	oxide	649:653	arg1	property					628:635	the surface property	616:635	the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan	616:716	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	2	38	theme	SEM	348:350	arg1	techniques					352:361	FT-IR, XRD, and SEM techniques	332:361	FT-IR, XRD, and SEM techniques	332:361	The nanocomposite was characterized by FT-IR, XRD, and SEM techniques.
26187194	1	39	from	solution	283:290	arg1	removal					247:253	the removal	243:253	the removal of Pb(II) ions from aqueous solution	243:290	In the present study, a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite was prepared and used as an adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	1	40	theme	ions	265:268	arg1	removal					247:253	the removal	243:253	the removal of Pb(II) ions from aqueous solution	243:290	In the present study, a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite was prepared and used as an adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	5	41	theme	time	967:970	arg1	pH					920:921	pH	920:921	pH of milieu, amount of adsorbent, and contact time	920:970	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
26187194	3	42	theme	shaking	477:483	arg1	speed					485:489	shaking speed	477:489	shaking speed (150-800 rpm)	477:503	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	42	theme	shaking	477:483	arg1	rpm					500:502	150-800 rpm	492:502	150-800 rpm	492:502	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	43	theme	ions	430:433	arg1	adsorption					409:418	the adsorption	405:418	the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg)	405:559	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	5	44	theme	amount	934:939	arg1	pH					920:921	pH	920:921	pH of milieu, amount of adsorbent, and contact time	920:970	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
26187194	0	45	from	solution	98:105	arg1	removal					67:73	the removal	63:73	the removal of Pb(II) from aqueous solution	63:105	Preparation of graphene oxide/chitosan/FeOOH nanocomposite for the removal of Pb(II) from aqueous solution.
26187194	8	46	theme	correlation	1264:1274	arg1	coefficient					1276:1286	higher correlation coefficient	1257:1286	higher correlation coefficient (R(2))	1257:1293	However, Langmuir model showed higher correlation coefficient (R(2)) than Freundlich model.
26187194	8	46	theme	correlation	1264:1274	arg1	R					1289:1289	R(2)	1289:1292	R(2)	1289:1292	However, Langmuir model showed higher correlation coefficient (R(2)) than Freundlich model.
26187194	9	47	theme	ions	1414:1417	arg1	removal					1396:1402	the removal	1392:1402	the removal of Pb(II) ions from aqueous solution	1392:1439	The study suggests that the GO/Ch/FeOOH is a promising nano adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	1	48	theme	aqueous	275:281	arg1	solution					283:290	aqueous solution	275:290	aqueous solution	275:290	In the present study, a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite was prepared and used as an adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	4	49	theme	adequate	742:749	arg1	adsorption					765:774	adequate and versatile adsorption	742:774	adequate and versatile adsorption	742:774	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	3	50	theme	sorbent	536:542	arg1	mass					544:547	sorbent mass	536:547	sorbent mass (10-100 mg)	536:559	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	50	theme	sorbent	536:542	arg1	mg					557:558	10-100 mg	550:558	10-100 mg	550:558	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	6	51	theme	adsorption	1001:1010	arg1	models					1012:1017	The Freundlich and Langmuir adsorption models	973:1017	The Freundlich and Langmuir adsorption models	973:1017	The Freundlich and Langmuir adsorption models were used for the mathematical description of adsorption equilibrium and isotherm constants.
26187194	7	52	theme	isotherm	1181:1188	arg1	description					1148:1158	the description	1144:1158	the description of Pb(II) adsorption isotherm in the concentration range studied	1144:1223	Both models were applicable for the description of Pb(II) adsorption isotherm in the concentration range studied.
26187194	3	53	theme	II	426:427	arg1	pH					443:444	pH	443:444	pH (3-7)	443:450	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	53	theme	II	426:427	arg1	time					514:517	contact time	506:517	contact time (10-70 min)	506:529	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	53	theme	II	426:427	arg1	mass					544:547	sorbent mass	536:547	sorbent mass (10-100 mg)	536:559	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	53	theme	II	426:427	arg1	ions					430:433	Pb(II) ions	423:433	Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg)	423:559	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	53	theme	II	426:427	arg1	temperature					453:463	temperature	453:463	temperature (25-80 °C)	453:474	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	53	theme	II	426:427	arg1	speed					485:489	shaking speed	477:489	shaking speed (150-800 rpm)	477:503	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	6	54	theme	constants	1101:1109	arg1	description					1050:1060	the mathematical description	1033:1060	the mathematical description of adsorption equilibrium and isotherm constants	1033:1109	The Freundlich and Langmuir adsorption models were used for the mathematical description of adsorption equilibrium and isotherm constants.
26187194	1	55	used	used	218:221	arg2	composite					191:199	a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite	130:199	a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite	130:199	In the present study, a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite was prepared and used as an adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	1	55	used	used	218:221	arg2	adsorbent					229:237	adsorbent	229:237	adsorbent	229:237	In the present study, a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite was prepared and used as an adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	3	56	theme	contact	506:512	arg1	time					514:517	contact time	506:517	contact time (10-70 min)	506:529	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	3	56	theme	contact	506:512	arg1	min					526:528	10-70 min	520:528	10-70 min	520:528	Several important parameters influencing the adsorption of Pb(II) ions such as pH (3-7), temperature (25-80 °C), shaking speed (150-800 rpm), contact time (10-70 min), and sorbent mass (10-100 mg) were studied.
26187194	1	57	theme	present	115:121	arg1	study					123:127	the present study	111:127	the present study	111:127	In the present study, a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite was prepared and used as an adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	0	58	theme	Pb	78:79	arg1	removal					67:73	the removal	63:73	the removal of Pb(II) from aqueous solution	63:105	Preparation of graphene oxide/chitosan/FeOOH nanocomposite for the removal of Pb(II) from aqueous solution.
26187194	5	59	theme	Pb	884:885	arg1	adsorption					866:875	the adsorption	862:875	the adsorption of the Pb(II)	862:889	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
26187194	5	59	theme	Pb	884:885	arg1	dependent					907:915	dependent	907:915	dependent	907:915	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
26187194	4	60	theme	graphene	640:647	arg1	amino					669:673	the abundant amino and hydroxyl functional groups	656:704	amino	669:673	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	60	theme	graphene	640:647	arg1	chitosan					709:716	chitosan	709:716	chitosan	709:716	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	60	theme	graphene	640:647	arg1	groups					699:704	the abundant amino and hydroxyl functional groups	656:704	groups	699:704	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	4	60	theme	graphene	640:647	arg1	oxide					649:653	graphene oxide	640:653	graphene oxide	640:653	The results showed that, benefiting from the surface property of graphene oxide, the abundant amino and hydroxyl functional groups of chitosan, the adsorbent provides adequate and versatile adsorption for the Pb(II) ions under investigation.
26187194	1	61	theme	nanostructured	176:189	arg1	composite					191:199	a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite	130:199	a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite	130:199	In the present study, a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite was prepared and used as an adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	1	61	theme	nanostructured	176:189	arg1	adsorbent					229:237	adsorbent	229:237	adsorbent	229:237	In the present study, a graphene oxide/chitosan/FeOOH (GO/Ch/FeOOH) nanostructured composite was prepared and used as an adsorbent for the removal of Pb(II) ions from aqueous solution.
26187194	2	62	theme	FT-IR	332:336	arg1	techniques					352:361	FT-IR, XRD, and SEM techniques	332:361	FT-IR, XRD, and SEM techniques	332:361	The nanocomposite was characterized by FT-IR, XRD, and SEM techniques.
26187194	5	63	theme	adsorption	827:836	arg1	experiments					838:848	The batch adsorption experiments	817:848	The batch adsorption experiments	817:848	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
26187194	5	64	theme	adsorbent	944:952	arg1	milieu					926:931	milieu	926:931	milieu	926:931	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
26187194	5	64	theme	adsorbent	944:952	arg1	amount					934:939	amount	934:939	amount of adsorbent	934:952	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
26187194	5	64	theme	adsorbent	944:952	arg1	adsorbent					944:952	adsorbent	944:952	adsorbent	944:952	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
26187194	5	64	theme	adsorbent	944:952	arg1	time					967:970	contact time	959:970	contact time	959:970	The batch adsorption experiments showed that the adsorption of the Pb(II) is considerably dependent on pH of milieu, amount of adsorbent, and contact time.
24507339	8	0	theme	agar-based	1299:1308	arg1	films					1310:1314	the agar-based films	1295:1314	the agar-based films	1295:1314	The addition of GSE changed film microstructure of the film, but did not influence the crystallinity of agar and thermal stability of the agar-based films.
24507339	9	1	theme	agar/GSE	1321:1328	arg1	films					1330:1334	The agar/GSE films	1317:1334	The agar/GSE films	1317:1334	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	1	2	theme	serious	172:178	arg1	problems					194:201	serious environmental problems	172:201	serious environmental problems due to their difficulty in recycling and poor biodegradability	172:264	The use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability.
24507339	0	3	theme	seed	98:101	arg1	extract					103:109	grapefruit seed extract	87:109	grapefruit seed extract	87:109	Antimicrobial and physical-mechanical properties of agar-based films incorporated with grapefruit seed extract.
24507339	7	4	dep	13.3	1068:1071	arg1	to					1065:1066	to	1065:1066	to	1065:1066	As the concentration of GSE increased from 0.6 to 13.3 μg/mL, the physical and mechanical properties of the films were affected significantly.
24507339	1	5	theme	environmental	180:192	arg1	problems					194:201	serious environmental problems	172:201	serious environmental problems due to their difficulty in recycling and poor biodegradability	172:264	The use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability.
24507339	10	6	theme	food	1567:1570	arg1	systems					1582:1588	an active food packaging systems	1557:1588	an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food	1557:1666	These results suggest that agar/GSE films have potential to be used in an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food.
24507339	6	7	theme	vapor	905:909	arg1	permeability					911:922	water vapor permeability	899:922	water vapor permeability	899:922	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	7	theme	vapor	905:909	arg1	color					841:845	color	841:845	color	841:845	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	2	8	theme	natural	313:319	arg1	alternative					374:384	an alternative	371:384	an alternative for the synthetic packaging films	371:418	Therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films.
24507339	2	8	theme	natural	313:319	arg1	films					362:366	natural biopolymer-based antimicrobial packaging films	313:366	natural biopolymer-based antimicrobial packaging films	313:366	Therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films.
24507339	4	9	theme	solvent	624:630	arg1	method					640:645	a solvent casting method	622:645	a solvent casting method	622:645	The films with different concentrations of GSE were prepared by a solvent casting method and the resulting composite films were examined physically and mechanically.
24507339	10	10	theme	active	1560:1565	arg1	systems					1582:1588	an active food packaging systems	1557:1588	an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food	1557:1666	These results suggest that agar/GSE films have potential to be used in an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food.
24507339	6	11	theme	water	899:903	arg1	permeability					911:922	water vapor permeability	899:922	water vapor permeability	899:922	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	11	theme	water	899:903	arg1	color					841:845	color	841:845	color	841:845	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	7	12	theme	mechanical	1097:1106	arg1	properties					1108:1117	the physical and mechanical properties	1080:1117	the physical and mechanical properties of the films	1080:1130	As the concentration of GSE increased from 0.6 to 13.3 μg/mL, the physical and mechanical properties of the films were affected significantly.
24507339	2	13	theme	synthetic	394:402	arg1	films					414:418	the synthetic packaging films	390:418	the synthetic packaging films	390:418	Therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films.
24507339	9	14	theme	distinctive	1346:1356	arg1	activity					1372:1379	distinctive antimicrobial activity	1346:1379	distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli	1346:1483	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	6	15	theme	elastic	988:994	arg1	modulus					996:1002	elastic modulus	988:1002	elastic modulus of the films	988:1015	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	10	16	contain	have	1528:1531	arg2	potential					1533:1541	potential	1533:1541	potential to be used in an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food	1533:1666	These results suggest that agar/GSE films have potential to be used in an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food.
24507339	10	16	contain	have	1528:1531	arg1	films					1522:1526	agar/GSE films	1513:1526	agar/GSE films	1513:1526	These results suggest that agar/GSE films have potential to be used in an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food.
24507339	6	17	theme	GSE	818:820	arg1	incorporation					801:813	The incorporation	797:813	The incorporation of GSE	797:820	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	8	18	theme	films	1310:1314	arg1	agar					1265:1268	agar	1265:1268	agar	1265:1268	The addition of GSE changed film microstructure of the film, but did not influence the crystallinity of agar and thermal stability of the agar-based films.
24507339	8	18	theme	films	1310:1314	arg1	stability					1282:1290	thermal stability	1274:1290	thermal stability	1274:1290	The addition of GSE changed film microstructure of the film, but did not influence the crystallinity of agar and thermal stability of the agar-based films.
24507339	7	19	theme	films	1126:1130	arg1	properties					1108:1117	the physical and mechanical properties	1080:1117	the physical and mechanical properties of the films	1080:1130	As the concentration of GSE increased from 0.6 to 13.3 μg/mL, the physical and mechanical properties of the films were affected significantly.
24507339	7	20	theme	physical	1084:1091	arg1	properties					1108:1117	the physical and mechanical properties	1080:1117	the physical and mechanical properties of the films	1080:1130	As the concentration of GSE increased from 0.6 to 13.3 μg/mL, the physical and mechanical properties of the films were affected significantly.
24507339	1	21	theme	due	203:205	arg1	problems					194:201	serious environmental problems	172:201	serious environmental problems due to their difficulty in recycling and poor biodegradability	172:264	The use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability.
24507339	8	22	theme	GSE	1177:1179	arg1	addition					1165:1172	The addition	1161:1172	The addition of GSE	1161:1179	The addition of GSE changed film microstructure of the film, but did not influence the crystallinity of agar and thermal stability of the agar-based films.
24507339	6	23	theme	water	878:882	arg1	color					841:845	color	841:845	color	841:845	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	23	theme	water	878:882	arg1	solubility					884:893	water solubility	878:893	water solubility	878:893	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	3	24	theme	seed	466:469	arg1	agent					448:452	a natural antimicrobial agent	424:452	a natural antimicrobial agent	424:452	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	3	24	theme	seed	466:469	arg1	GSE					480:482	GSE	480:482	GSE	480:482	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	3	24	theme	seed	466:469	arg1	extract					471:477	grapefruit seed extract	455:477	grapefruit seed extract (GSE)	455:483	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	3	25	theme	natural	426:432	arg1	agent					448:452	a natural antimicrobial agent	424:452	a natural antimicrobial agent	424:452	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	3	25	theme	natural	426:432	arg1	extract					471:477	grapefruit seed extract	455:477	grapefruit seed extract (GSE)	455:483	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	10	26	theme	food	1606:1609	arg1	safety					1611:1616	food safety	1606:1616	food safety	1606:1616	These results suggest that agar/GSE films have potential to be used in an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food.
24507339	9	27	theme	antimicrobial	1358:1370	arg1	activity					1372:1379	distinctive antimicrobial activity	1346:1379	distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli	1346:1483	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	2	28	theme	packaging	352:360	arg1	alternative					374:384	an alternative	371:384	an alternative for the synthetic packaging films	371:418	Therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films.
24507339	2	28	theme	packaging	352:360	arg1	films					362:366	natural biopolymer-based antimicrobial packaging films	313:366	natural biopolymer-based antimicrobial packaging films	313:366	Therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films.
24507339	6	29	from	increase	829:836	arg1	permeability					911:922	water vapor permeability	899:922	water vapor permeability	899:922	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	29	from	increase	829:836	arg1	color					841:845	color	841:845	color	841:845	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	29	from	increase	829:836	arg1	barrier					851:857	UV barrier	848:857	UV barrier	848:857	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	29	from	increase	829:836	arg1	content					869:875	moisture content	860:875	moisture content	860:875	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	29	from	increase	829:836	arg1	solubility					884:893	water solubility	878:893	water solubility	878:893	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	8	30	theme	film	1189:1192	arg1	microstructure					1194:1207	film microstructure	1189:1207	film microstructure of the film	1189:1219	The addition of GSE changed film microstructure of the film, but did not influence the crystallinity of agar and thermal stability of the agar-based films.
24507339	8	31	theme	stability	1282:1290	arg1	crystallinity					1248:1260	the crystallinity	1244:1260	the crystallinity of agar and thermal stability of the agar-based films	1244:1314	The addition of GSE changed film microstructure of the film, but did not influence the crystallinity of agar and thermal stability of the agar-based films.
24507339	9	32	theme	Listeria	1424:1431	arg1	monocytogenes					1433:1445	Listeria monocytogenes	1424:1445	Listeria monocytogenes	1424:1445	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	2	33	theme	antimicrobial	338:350	arg1	alternative					374:384	an alternative	371:384	an alternative for the synthetic packaging films	371:418	Therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films.
24507339	2	33	theme	antimicrobial	338:350	arg1	films					362:366	natural biopolymer-based antimicrobial packaging films	313:366	natural biopolymer-based antimicrobial packaging films	313:366	Therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films.
24507339	4	34	theme	composite	665:673	arg1	films					675:679	the resulting composite films	651:679	the resulting composite films	651:679	The films with different concentrations of GSE were prepared by a solvent casting method and the resulting composite films were examined physically and mechanically.
24507339	0	35	theme	physical-mechanical	18:36	arg1	properties					38:47	physical-mechanical properties	18:47	physical-mechanical properties	18:47	Antimicrobial and physical-mechanical properties of agar-based films incorporated with grapefruit seed extract.
24507339	6	36	theme	tensile	967:973	arg1	strength					975:982	tensile strength	967:982	tensile strength	967:982	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	2	37	theme	biopolymer-based	321:336	arg1	alternative					374:384	an alternative	371:384	an alternative for the synthetic packaging films	371:418	Therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films.
24507339	2	37	theme	biopolymer-based	321:336	arg1	films					362:366	natural biopolymer-based antimicrobial packaging films	313:366	natural biopolymer-based antimicrobial packaging films	313:366	Therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films.
24507339	4	38	theme	resulting	655:663	arg1	films					675:679	the resulting composite films	651:679	the resulting composite films	651:679	The films with different concentrations of GSE were prepared by a solvent casting method and the resulting composite films were examined physically and mechanically.
24507339	0	39	theme	agar-based	52:61	arg1	films					63:67	agar-based films	52:67	agar-based films	52:67	Antimicrobial and physical-mechanical properties of agar-based films incorporated with grapefruit seed extract.
24507339	4	40	theme	GSE	601:603	arg1	concentrations					583:596	different concentrations	573:596	different concentrations of GSE	573:603	The films with different concentrations of GSE were prepared by a solvent casting method and the resulting composite films were examined physically and mechanically.
24507339	10	41	theme	packaging	1572:1580	arg1	systems					1582:1588	an active food packaging systems	1557:1588	an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food	1557:1666	These results suggest that agar/GSE films have potential to be used in an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food.
24507339	9	42	theme	food	1400:1403	arg1	cereus					1457:1462	Bacillus cereus	1448:1462	Bacillus cereus	1448:1462	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	9	42	theme	food	1400:1403	arg1	monocytogenes					1433:1445	Listeria monocytogenes	1424:1445	Listeria monocytogenes	1424:1445	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	9	42	theme	food	1400:1403	arg1	pathogens					1405:1413	three test food pathogens	1389:1413	three test food pathogens	1389:1413	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	9	42	theme	food	1400:1403	arg1	coli					1480:1483	Escherichia coli	1468:1483	Escherichia coli	1468:1483	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	10	43	theme	food	1663:1666	arg1	shelf-life					1636:1645	the shelf-life	1632:1645	the shelf-life of the packaged food	1632:1666	These results suggest that agar/GSE films have potential to be used in an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food.
24507339	3	44	theme	grapefruit	455:464	arg1	agent					448:452	a natural antimicrobial agent	424:452	a natural antimicrobial agent	424:452	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	3	44	theme	grapefruit	455:464	arg1	GSE					480:482	GSE	480:482	GSE	480:482	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	3	44	theme	grapefruit	455:464	arg1	extract					471:477	grapefruit seed extract	455:477	grapefruit seed extract (GSE)	455:483	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	6	45	theme	moisture	860:867	arg1	color					841:845	color	841:845	color	841:845	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	45	theme	moisture	860:867	arg1	content					869:875	moisture content	860:875	moisture content	860:875	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	8	46	theme	thermal	1274:1280	arg1	stability					1282:1290	thermal stability	1274:1290	thermal stability	1274:1290	The addition of GSE changed film microstructure of the film, but did not influence the crystallinity of agar and thermal stability of the agar-based films.
24507339	3	47	theme	antimicrobial	434:446	arg1	agent					448:452	a natural antimicrobial agent	424:452	a natural antimicrobial agent	424:452	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	3	47	theme	antimicrobial	434:446	arg1	extract					471:477	grapefruit seed extract	455:477	grapefruit seed extract (GSE)	455:483	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	3	48	theme	antimicrobial	528:540	arg1	film					552:555	antimicrobial packaging film	528:555	antimicrobial packaging film	528:555	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	7	49	theme	GSE	1042:1044	arg1	concentration					1025:1037	the concentration	1021:1037	the concentration of GSE	1021:1044	As the concentration of GSE increased from 0.6 to 13.3 μg/mL, the physical and mechanical properties of the films were affected significantly.
24507339	1	50	theme	synthetic	123:131	arg1	films					159:163	synthetic petroleum based packaging films	123:163	synthetic petroleum based packaging films	123:163	The use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability.
24507339	0	51	theme	films	63:67	arg1	Antimicrobial					0:12	Antimicrobial	0:12	Antimicrobial	0:12	Antimicrobial and physical-mechanical properties of agar-based films incorporated with grapefruit seed extract.
24507339	0	51	theme	films	63:67	arg1	properties					38:47	physical-mechanical properties	18:47	physical-mechanical properties	18:47	Antimicrobial and physical-mechanical properties of agar-based films incorporated with grapefruit seed extract.
24507339	4	52	theme	different	573:581	arg1	concentrations					583:596	different concentrations	573:596	different concentrations of GSE	573:603	The films with different concentrations of GSE were prepared by a solvent casting method and the resulting composite films were examined physically and mechanically.
24507339	6	53	from	decrease	931:938	arg1	hydrophobicity					951:964	surface hydrophobicity	943:964	surface hydrophobicity	943:964	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	53	from	decrease	931:938	arg1	strength					975:982	tensile strength	967:982	tensile strength	967:982	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	53	from	decrease	931:938	arg1	modulus					996:1002	elastic modulus	988:1002	elastic modulus of the films	988:1015	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	54	theme	UV	848:849	arg1	color					841:845	color	841:845	color	841:845	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	54	theme	UV	848:849	arg1	barrier					851:857	UV barrier	848:857	UV barrier	848:857	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	1	55	from	difficulty	216:225	arg1	recycling					230:238	recycling	230:238	recycling	230:238	The use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability.
24507339	1	55	from	difficulty	216:225	arg1	biodegradability					249:264	poor biodegradability	244:264	poor biodegradability	244:264	The use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability.
24507339	1	56	theme	petroleum	133:141	arg1	films					159:163	synthetic petroleum based packaging films	123:163	synthetic petroleum based packaging films	123:163	The use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability.
24507339	10	57	theme	agar/GSE	1513:1520	arg1	films					1522:1526	agar/GSE films	1513:1526	agar/GSE films	1513:1526	These results suggest that agar/GSE films have potential to be used in an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food.
24507339	3	58	theme	packaging	542:550	arg1	film					552:555	antimicrobial packaging film	528:555	antimicrobial packaging film	528:555	As a natural antimicrobial agent, grapefruit seed extract (GSE) has been incorporated into agar to prepare antimicrobial packaging film.
24507339	1	59	theme	based	143:147	arg1	films					159:163	synthetic petroleum based packaging films	123:163	synthetic petroleum based packaging films	123:163	The use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability.
24507339	4	60	with	films	562:566	arg1	concentrations					583:596	different concentrations	573:596	different concentrations of GSE	573:603	The films with different concentrations of GSE were prepared by a solvent casting method and the resulting composite films were examined physically and mechanically.
24507339	1	61	theme	poor	244:247	arg1	biodegradability					249:264	poor biodegradability	244:264	poor biodegradability	244:264	The use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability.
24507339	1	62	theme	packaging	149:157	arg1	films					159:163	synthetic petroleum based packaging films	123:163	synthetic petroleum based packaging films	123:163	The use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability.
24507339	4	63	theme	casting	632:638	arg1	method					640:645	a solvent casting method	622:645	a solvent casting method	622:645	The films with different concentrations of GSE were prepared by a solvent casting method and the resulting composite films were examined physically and mechanically.
24507339	0	64	theme	grapefruit	87:96	arg1	extract					103:109	grapefruit seed extract	87:109	grapefruit seed extract	87:109	Antimicrobial and physical-mechanical properties of agar-based films incorporated with grapefruit seed extract.
24507339	10	65	theme	packaged	1654:1661	arg1	food					1663:1666	the packaged food	1650:1666	the packaged food	1650:1666	These results suggest that agar/GSE films have potential to be used in an active food packaging systems for maintaining food safety and extending the shelf-life of the packaged food.
24507339	2	66	theme	present	278:284	arg1	study					286:290	present study	278:290	present study	278:290	Therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films.
24507339	1	67	theme	films	159:163	arg1	use					116:118	The use	112:118	The use of synthetic petroleum based packaging films	112:163	The use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability.
24507339	9	68	theme	test	1395:1398	arg1	cereus					1457:1462	Bacillus cereus	1448:1462	Bacillus cereus	1448:1462	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	9	68	theme	test	1395:1398	arg1	monocytogenes					1433:1445	Listeria monocytogenes	1424:1445	Listeria monocytogenes	1424:1445	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	9	68	theme	test	1395:1398	arg1	pathogens					1405:1413	three test food pathogens	1389:1413	three test food pathogens	1389:1413	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	9	68	theme	test	1395:1398	arg1	coli					1480:1483	Escherichia coli	1468:1483	Escherichia coli	1468:1483	The agar/GSE films exhibited distinctive antimicrobial activity against three test food pathogens, such as Listeria monocytogenes, Bacillus cereus and Escherichia coli.
24507339	8	69	theme	film	1216:1219	arg1	microstructure					1194:1207	film microstructure	1189:1207	film microstructure of the film	1189:1219	The addition of GSE changed film microstructure of the film, but did not influence the crystallinity of agar and thermal stability of the agar-based films.
24507339	6	70	theme	films	1011:1015	arg1	hydrophobicity					951:964	surface hydrophobicity	943:964	surface hydrophobicity	943:964	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	70	theme	films	1011:1015	arg1	strength					975:982	tensile strength	967:982	tensile strength	967:982	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	6	70	theme	films	1011:1015	arg1	modulus					996:1002	elastic modulus	988:1002	elastic modulus of the films	988:1015	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
24507339	2	71	theme	packaging	404:412	arg1	films					414:418	the synthetic packaging films	390:418	the synthetic packaging films	390:418	Therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films.
24507339	8	72	theme	agar	1265:1268	arg1	crystallinity					1248:1260	the crystallinity	1244:1260	the crystallinity of agar and thermal stability of the agar-based films	1244:1314	The addition of GSE changed film microstructure of the film, but did not influence the crystallinity of agar and thermal stability of the agar-based films.
24507339	6	73	theme	surface	943:949	arg1	hydrophobicity					951:964	surface hydrophobicity	943:964	surface hydrophobicity	943:964	The incorporation of GSE caused increase in color, UV barrier, moisture content, water solubility and water vapor permeability, while decrease in surface hydrophobicity, tensile strength and elastic modulus of the films.
25293976	5	0	theme	chemical	714:721	arg1	treatment					723:731	chemical treatment	714:731	chemical treatment	714:731	This approach would help determine if the effect of chemical treatment on cellulose hydrogel improves the hydrophilicity, roughness, and rheological properties of the scaffold.
25293976	5	1	theme	treatment	723:731	arg1	effect					704:709	the effect	700:709	the effect of chemical treatment on cellulose hydrogel	700:753	This approach would help determine if the effect of chemical treatment on cellulose hydrogel improves the hydrophilicity, roughness, and rheological properties of the scaffold.
25293976	6	2	theme	biological	883:892	arg1	responses					894:902	the biological responses	879:902	the biological responses of human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation	879:998	In this study, it was demonstrated that the biological responses of human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation were influenced in vitro by changing the surface chemistry and roughness.
25293976	5	3	theme	rheological	799:809	arg1	properties					811:820	rheological properties	799:820	rheological properties	799:820	This approach would help determine if the effect of chemical treatment on cellulose hydrogel improves the hydrophilicity, roughness, and rheological properties of the scaffold.
25293976	0	4	from	Effect	0:5	arg1	differentiation					75:89	osteogenic differentiation	64:89	osteogenic differentiation	64:89	Effect of citric acid crosslinking cellulose-based hydrogels on osteogenic differentiation.
25293976	2	5	theme	non-toxic	336:344	arg1	acid					382:385	a non-toxic and natural component namely citric acid	334:385	a non-toxic and natural component namely citric acid	334:385	In this study, cellulose hydrogels were crosslinked using a non-toxic and natural component namely citric acid.
25293976	5	6	dep	hydrophilicity	768:781	arg1	the					764:766	the	764:766	the	764:766	This approach would help determine if the effect of chemical treatment on cellulose hydrogel improves the hydrophilicity, roughness, and rheological properties of the scaffold.
25293976	5	7	theme	cellulose	736:744	arg1	hydrogel					746:753	cellulose hydrogel	736:753	cellulose hydrogel	736:753	This approach would help determine if the effect of chemical treatment on cellulose hydrogel improves the hydrophilicity, roughness, and rheological properties of the scaffold.
25293976	4	8	theme	physiochemical	529:542	arg1	analyses					575:582	The physiochemical, morphological, and mechanical analyses	525:582	The physiochemical, morphological, and mechanical analyses	525:582	The physiochemical, morphological, and mechanical analyses were performed to analyze the material surface before and after crosslinking.
25293976	3	9	theme	roughness	476:484	arg1	properties					513:522	the hydrophilicity, roughness, and materials rheological properties	456:522	the hydrophilicity, roughness, and materials rheological properties	456:522	The chemical treatment induces COOH functional groups that improve the hydrophilicity, roughness, and materials rheological properties.
25293976	1	10	theme	designing	199:207	arg1	surfaces					226:233	designing optimal material surfaces	199:233	designing optimal material surfaces for implantation and tissue engineering	199:273	Understanding the relationships between material surface properties and cellular responses is essential to designing optimal material surfaces for implantation and tissue engineering.
25293976	3	11	theme	chemical	392:399	arg1	treatment					401:409	The chemical treatment	388:409	The chemical treatment	388:409	The chemical treatment induces COOH functional groups that improve the hydrophilicity, roughness, and materials rheological properties.
25293976	6	12	theme	cell	950:953	arg1	adhesion					955:962	cell adhesion	950:962	cell adhesion	950:962	In this study, it was demonstrated that the biological responses of human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation were influenced in vitro by changing the surface chemistry and roughness.
25293976	0	13	theme	cellulose-based	35:49	arg1	hydrogels					51:59	citric acid crosslinking cellulose-based hydrogels	10:59	citric acid crosslinking cellulose-based hydrogels	10:59	Effect of citric acid crosslinking cellulose-based hydrogels on osteogenic differentiation.
25293976	1	14	theme	optimal	209:215	arg1	surfaces					226:233	designing optimal material surfaces	199:233	designing optimal material surfaces for implantation and tissue engineering	199:273	Understanding the relationships between material surface properties and cellular responses is essential to designing optimal material surfaces for implantation and tissue engineering.
25293976	4	15	theme	morphological	545:557	arg1	analyses					575:582	The physiochemical, morphological, and mechanical analyses	525:582	The physiochemical, morphological, and mechanical analyses	525:582	The physiochemical, morphological, and mechanical analyses were performed to analyze the material surface before and after crosslinking.
25293976	6	16	theme	surface	1041:1047	arg1	chemistry					1049:1057	the surface chemistry	1037:1057	the surface chemistry	1037:1057	In this study, it was demonstrated that the biological responses of human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation were influenced in vitro by changing the surface chemistry and roughness.
25293976	2	17	theme	component	358:366	arg1	acid					382:385	a non-toxic and natural component namely citric acid	334:385	a non-toxic and natural component namely citric acid	334:385	In this study, cellulose hydrogels were crosslinked using a non-toxic and natural component namely citric acid.
25293976	1	18	theme	material	217:224	arg1	surfaces					226:233	designing optimal material surfaces	199:233	designing optimal material surfaces for implantation and tissue engineering	199:273	Understanding the relationships between material surface properties and cellular responses is essential to designing optimal material surfaces for implantation and tissue engineering.
25293976	5	19	from	effect	704:709	arg1	hydrogel					746:753	cellulose hydrogel	736:753	cellulose hydrogel	736:753	This approach would help determine if the effect of chemical treatment on cellulose hydrogel improves the hydrophilicity, roughness, and rheological properties of the scaffold.
25293976	2	20	theme	natural	350:356	arg1	acid					382:385	a non-toxic and natural component namely citric acid	334:385	a non-toxic and natural component namely citric acid	334:385	In this study, cellulose hydrogels were crosslinked using a non-toxic and natural component namely citric acid.
25293976	0	21	theme	acid	17:20	arg1	hydrogels					51:59	citric acid crosslinking cellulose-based hydrogels	10:59	citric acid crosslinking cellulose-based hydrogels	10:59	Effect of citric acid crosslinking cellulose-based hydrogels on osteogenic differentiation.
25293976	4	22	theme	material	614:621	arg1	surface					623:629	the material surface	610:629	the material surface	610:629	The physiochemical, morphological, and mechanical analyses were performed to analyze the material surface before and after crosslinking.
25293976	0	23	theme	citric	10:15	arg1	hydrogels					51:59	citric acid crosslinking cellulose-based hydrogels	10:59	citric acid crosslinking cellulose-based hydrogels	10:59	Effect of citric acid crosslinking cellulose-based hydrogels on osteogenic differentiation.
25293976	1	24	theme	material	132:139	arg1	properties					149:158	material surface properties	132:158	material surface properties	132:158	Understanding the relationships between material surface properties and cellular responses is essential to designing optimal material surfaces for implantation and tissue engineering.
25293976	3	25	theme	hydrophilicity	460:473	arg1	properties					513:522	the hydrophilicity, roughness, and materials rheological properties	456:522	the hydrophilicity, roughness, and materials rheological properties	456:522	The chemical treatment induces COOH functional groups that improve the hydrophilicity, roughness, and materials rheological properties.
25293976	3	26	theme	rheological	501:511	arg1	properties					513:522	the hydrophilicity, roughness, and materials rheological properties	456:522	the hydrophilicity, roughness, and materials rheological properties	456:522	The chemical treatment induces COOH functional groups that improve the hydrophilicity, roughness, and materials rheological properties.
25293976	0	27	theme	crosslinking	22:33	arg1	hydrogels					51:59	citric acid crosslinking cellulose-based hydrogels	10:59	citric acid crosslinking cellulose-based hydrogels	10:59	Effect of citric acid crosslinking cellulose-based hydrogels on osteogenic differentiation.
25293976	6	28	theme	cell	930:933	arg1	responses					894:902	the biological responses	879:902	the biological responses of human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation	879:998	In this study, it was demonstrated that the biological responses of human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation were influenced in vitro by changing the surface chemistry and roughness.
25293976	1	29	theme	tissue	256:261	arg1	engineering					263:273	tissue engineering	256:273	tissue engineering	256:273	Understanding the relationships between material surface properties and cellular responses is essential to designing optimal material surfaces for implantation and tissue engineering.
25293976	6	30	theme	stem	925:928	arg1	cell					930:933	human mesenchymal stem cell	907:933	human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation	907:998	In this study, it was demonstrated that the biological responses of human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation were influenced in vitro by changing the surface chemistry and roughness.
25293976	1	31	theme	surface	141:147	arg1	properties					149:158	material surface properties	132:158	material surface properties	132:158	Understanding the relationships between material surface properties and cellular responses is essential to designing optimal material surfaces for implantation and tissue engineering.
25293976	0	32	theme	hydrogels	51:59	arg1	Effect					0:5	Effect	0:5	Effect of citric acid crosslinking cellulose-based hydrogels on osteogenic differentiation.	0:90	Effect of citric acid crosslinking cellulose-based hydrogels on osteogenic differentiation.
25293976	6	33	theme	mesenchymal	913:923	arg1	cell					930:933	human mesenchymal stem cell	907:933	human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation	907:998	In this study, it was demonstrated that the biological responses of human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation were influenced in vitro by changing the surface chemistry and roughness.
25293976	2	34	attach	crosslinked	316:326	arg3	study					284:288	this study	279:288	this study	279:288	In this study, cellulose hydrogels were crosslinked using a non-toxic and natural component namely citric acid.
25293976	6	35	theme	human	907:911	arg1	cell					930:933	human mesenchymal stem cell	907:933	human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation	907:998	In this study, it was demonstrated that the biological responses of human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation were influenced in vitro by changing the surface chemistry and roughness.
25293976	2	36	theme	citric	375:380	arg1	acid					382:385	a non-toxic and natural component namely citric acid	334:385	a non-toxic and natural component namely citric acid	334:385	In this study, cellulose hydrogels were crosslinked using a non-toxic and natural component namely citric acid.
25293976	2	37	theme	cellulose	291:299	arg1	hydrogels					301:309	cellulose hydrogels	291:309	cellulose hydrogels	291:309	In this study, cellulose hydrogels were crosslinked using a non-toxic and natural component namely citric acid.
25293976	1	38	theme	cellular	164:171	arg1	responses					173:181	cellular responses	164:181	cellular responses	164:181	Understanding the relationships between material surface properties and cellular responses is essential to designing optimal material surfaces for implantation and tissue engineering.
25293976	5	39	theme	scaffold	829:836	arg1	roughness					784:792	roughness	784:792	roughness	784:792	This approach would help determine if the effect of chemical treatment on cellulose hydrogel improves the hydrophilicity, roughness, and rheological properties of the scaffold.
25293976	5	39	theme	scaffold	829:836	arg1	hydrophilicity					768:781	hydrophilicity	768:781	hydrophilicity	768:781	This approach would help determine if the effect of chemical treatment on cellulose hydrogel improves the hydrophilicity, roughness, and rheological properties of the scaffold.
25293976	5	39	theme	scaffold	829:836	arg1	properties					811:820	rheological properties	799:820	rheological properties	799:820	This approach would help determine if the effect of chemical treatment on cellulose hydrogel improves the hydrophilicity, roughness, and rheological properties of the scaffold.
25293976	3	40	theme	materials	491:499	arg1	properties					513:522	the hydrophilicity, roughness, and materials rheological properties	456:522	the hydrophilicity, roughness, and materials rheological properties	456:522	The chemical treatment induces COOH functional groups that improve the hydrophilicity, roughness, and materials rheological properties.
25293976	0	41	theme	osteogenic	64:73	arg1	differentiation					75:89	osteogenic differentiation	64:89	osteogenic differentiation	64:89	Effect of citric acid crosslinking cellulose-based hydrogels on osteogenic differentiation.
25293976	4	42	theme	mechanical	564:573	arg1	analyses					575:582	The physiochemical, morphological, and mechanical analyses	525:582	The physiochemical, morphological, and mechanical analyses	525:582	The physiochemical, morphological, and mechanical analyses were performed to analyze the material surface before and after crosslinking.
25293976	3	43	theme	functional	425:434	arg1	groups					436:441	COOH functional groups	419:441	COOH functional groups that improve the hydrophilicity, roughness, and materials rheological properties	419:522	The chemical treatment induces COOH functional groups that improve the hydrophilicity, roughness, and materials rheological properties.
26241746	4	0	from	24°C	745:748	arg1	h					740:740	96 h	737:740	96 h at 24°C	737:748	L. amazonensis promastigotes encapsulated in liposomes filled with RPMI-Dextran solution at pH 7.5 or 6.5 survived up to 96 h at 24°C.
26241746	4	1	theme	RPMI-Dextran	683:694	arg1	solution					696:703	RPMI-Dextran solution	683:703	RPMI-Dextran solution	683:703	L. amazonensis promastigotes encapsulated in liposomes filled with RPMI-Dextran solution at pH 7.5 or 6.5 survived up to 96 h at 24°C.
26241746	7	2	theme	biophysical	1059:1069	arg1	properties					1071:1080	biophysical properties	1059:1080	biophysical properties of the PV	1059:1090	This method will also facilitate the study of the temporal evolution of biophysical properties of the PV during its maturation.
26241746	1	3	theme	intracellular	181:193	arg1	PVs					221:223	PVs	221:223	PVs	221:223	After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
26241746	1	3	theme	intracellular	181:193	arg1	vacuoles					211:218	intracellular parasitophorous vacuoles	181:218	intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate	181:284	After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
26241746	0	4	from	Encapsulation	0:12	arg1	Vacuoles					69:76	Artificial Lipid Vacuoles	52:76	Artificial Lipid Vacuoles	52:76	Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles.
26241746	7	5	theme	temporal	1037:1044	arg1	evolution					1046:1054	the temporal evolution	1033:1054	the temporal evolution of biophysical properties of the PV	1033:1090	This method will also facilitate the study of the temporal evolution of biophysical properties of the PV during its maturation.
26241746	3	6	theme	liposomes	497:505	arg1	composition					523:533	the composition	519:533	the composition	519:533	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	3	6	theme	liposomes	497:505	arg1	number					569:574	the number	565:574	the number of internalized parasites per liposome	565:613	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	3	6	theme	liposomes	497:505	arg1	pH					512:513	pH	512:513	pH	512:513	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	3	6	theme	liposomes	497:505	arg1	size					489:492	the size	485:492	the size of liposomes	485:505	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	6	7	theme	certain	836:842	arg1	effectors					844:852	identifying certain effectors	824:852	identifying certain effectors secreted by the parasite	824:877	This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell.
26241746	7	8	theme	evolution	1046:1054	arg1	study					1024:1028	the study	1020:1028	the study of the temporal evolution of biophysical properties of the PV	1020:1090	This method will also facilitate the study of the temporal evolution of biophysical properties of the PV during its maturation.
26241746	1	9	theme	Leishmania	146:155	arg1	parasites					157:165	Leishmania parasites	146:165	Leishmania parasites	146:165	After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
26241746	6	10	theme	identifying	824:834	arg1	effectors					844:852	identifying certain effectors	824:852	identifying certain effectors secreted by the parasite	824:877	This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell.
26241746	1	11	theme	parasitophorous	195:209	arg1	PVs					221:223	PVs	221:223	PVs	221:223	After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
26241746	1	11	theme	parasitophorous	195:209	arg1	vacuoles					211:218	intracellular parasitophorous vacuoles	181:218	intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate	181:284	After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
26241746	4	12	theme	amazonensis	619:629	arg1	promastigotes					631:643	L. amazonensis promastigotes	616:643	L. amazonensis promastigotes encapsulated in liposomes filled with RPMI-Dextran solution at pH 7.5 or 6.5	616:720	L. amazonensis promastigotes encapsulated in liposomes filled with RPMI-Dextran solution at pH 7.5 or 6.5 survived up to 96 h at 24°C.
26241746	2	13	theme	model	450:454	arg1	PVs					456:458	model PVs	450:458	model PVs	450:458	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	2	13	theme	model	450:454	arg1	liposomes					426:434	giant artificial liposomes	409:434	giant artificial liposomes that serve as model PVs	409:458	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	6	14	theme	specific	897:904	arg1	mechanisms					906:915	unraveling specific mechanisms	886:915	unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell	886:984	This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell.
26241746	2	15	theme	inverted	317:324	arg1	method					336:341	the 'inverted emulsion' method	312:341	the 'inverted emulsion' method	312:341	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	4	16	dep	h	740:740	arg1	up					731:732	up	731:732	up	731:732	L. amazonensis promastigotes encapsulated in liposomes filled with RPMI-Dextran solution at pH 7.5 or 6.5 survived up to 96 h at 24°C.
26241746	2	17	theme	L.	381:382	arg1	parasites					396:404	living L. amazonensis parasites	374:404	living L. amazonensis parasites	374:404	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	6	18	theme	fusion	920:925	arg1	mechanisms					906:915	unraveling specific mechanisms	886:915	unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell	886:984	This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell.
26241746	1	19	theme	mammalian	101:109	arg1	macrophages					111:121	mammalian macrophages	101:121	mammalian macrophages	101:121	After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
26241746	2	20	theme	living	374:379	arg1	parasites					396:404	living L. amazonensis parasites	374:404	living L. amazonensis parasites	374:404	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	0	21	theme	Leishmania	24:33	arg1	Promastigotes					35:47	Leishmania Promastigotes	24:47	Living Leishmania Promastigotes	17:47	Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles.
26241746	6	22	theme	cell	981:984	arg1	PV					939:940	PV	939:940	PV	939:940	This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell.
26241746	6	22	theme	cell	981:984	arg1	vesicles					960:967	intracellular vesicles	946:967	intracellular vesicles	946:967	This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell.
26241746	0	23	theme	Living	17:22	arg1	Promastigotes					35:47	Leishmania Promastigotes	24:47	Living Leishmania Promastigotes	17:47	Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles.
26241746	2	24	theme	artificial	415:424	arg1	PVs					456:458	model PVs	450:458	model PVs	450:458	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	2	24	theme	artificial	415:424	arg1	liposomes					426:434	giant artificial liposomes	409:434	giant artificial liposomes that serve as model PVs	409:458	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	3	25	dep	pH	512:513	arg1	the					508:510	the	508:510	the	508:510	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	4	26	theme	L.	616:617	arg1	promastigotes					631:643	L. amazonensis promastigotes	616:643	L. amazonensis promastigotes encapsulated in liposomes filled with RPMI-Dextran solution at pH 7.5 or 6.5	616:720	L. amazonensis promastigotes encapsulated in liposomes filled with RPMI-Dextran solution at pH 7.5 or 6.5 survived up to 96 h at 24°C.
26241746	2	27	theme	giant	409:413	arg1	PVs					456:458	model PVs	450:458	model PVs	450:458	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	2	27	theme	giant	409:413	arg1	liposomes					426:434	giant artificial liposomes	409:434	giant artificial liposomes that serve as model PVs	409:458	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	0	28	theme	Promastigotes	35:47	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles.	0:77	Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles.
26241746	6	29	theme	unraveling	886:895	arg1	mechanisms					906:915	unraveling specific mechanisms	886:915	unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell	886:984	This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell.
26241746	7	30	theme	PV	1089:1090	arg1	properties					1071:1080	biophysical properties	1059:1080	biophysical properties of the PV	1059:1090	This method will also facilitate the study of the temporal evolution of biophysical properties of the PV during its maturation.
26241746	6	31	theme	host	976:979	arg1	cell					981:984	the host cell	972:984	the host cell	972:984	This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell.
26241746	0	32	theme	Lipid	63:67	arg1	Vacuoles					69:76	Artificial Lipid Vacuoles	52:76	Artificial Lipid Vacuoles	52:76	Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles.
26241746	3	33	theme	volume	553:558	arg1	composition					523:533	the composition	519:533	the composition	519:533	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	3	33	theme	volume	553:558	arg1	number					569:574	the number	565:574	the number of internalized parasites per liposome	565:613	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	3	33	theme	volume	553:558	arg1	pH					512:513	pH	512:513	pH	512:513	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	3	33	theme	volume	553:558	arg1	size					489:492	the size	485:492	the size of liposomes	485:505	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	1	34	theme	promastigote	124:135	arg1	forms					137:141	promastigote forms	124:141	promastigote forms of Leishmania parasites	124:165	After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
26241746	0	35	theme	Artificial	52:61	arg1	Vacuoles					69:76	Artificial Lipid Vacuoles	52:76	Artificial Lipid Vacuoles	52:76	Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles.
26241746	6	36	dep	PV	939:940	arg1	the					935:937	the	935:937	the	935:937	This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell.
26241746	2	37	theme	method	336:341	arg1	variant					301:307	a variant	299:307	a variant of the 'inverted emulsion' method	299:341	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	3	38	theme	internalized	579:590	arg1	parasites					592:600	internalized parasites	579:600	internalized parasites	579:600	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	2	39	dep	L.	381:382	arg1	amazonensis					384:394	amazonensis	384:394	amazonensis	384:394	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	3	40	theme	parasites	592:600	arg1	composition					523:533	the composition	519:533	the composition	519:533	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	3	40	theme	parasites	592:600	arg1	number					569:574	the number	565:574	the number of internalized parasites per liposome	565:613	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	3	40	theme	parasites	592:600	arg1	pH					512:513	pH	512:513	pH	512:513	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	3	40	theme	parasites	592:600	arg1	size					489:492	the size	485:492	the size of liposomes	485:505	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	2	41	theme	emulsion	326:333	arg1	method					336:341	the 'inverted emulsion' method	312:341	the 'inverted emulsion' method	312:341	Here, using a variant of the 'inverted emulsion' method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs.
26241746	1	42	theme	parasites	157:165	arg1	forms					137:141	promastigote forms	124:141	promastigote forms of Leishmania parasites	124:165	After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
26241746	1	43	theme	amastigote	255:264	arg1	forms					266:270	amastigote forms	255:270	amastigote forms	255:270	After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
26241746	7	44	theme	properties	1071:1080	arg1	evolution					1046:1054	the temporal evolution	1033:1054	the temporal evolution of biophysical properties of the PV	1033:1090	This method will also facilitate the study of the temporal evolution of biophysical properties of the PV during its maturation.
26241746	3	45	theme	internal	544:551	arg1	volume					553:558	their internal volume	538:558	their internal volume	538:558	We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome.
26241746	6	46	theme	intracellular	946:958	arg1	vesicles					960:967	intracellular vesicles	946:967	intracellular vesicles	946:967	This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell.
25498760	8	0	theme	tested	1468:1473	arg1	mix					1481:1483	the tested fibre mix	1464:1483	the tested fibre mix	1464:1483	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	2	1	theme	microbial	331:339	arg1	composition					341:351	a disturbed microbial composition	319:351	a disturbed microbial composition	319:351	The interaction between a disturbed microbial composition, the intestinal mucosal barrier and the mucosal immune system plays an important role in IBD and its chronicity.
25498760	6	2	theme	observed	1302:1309	arg1	effects					1311:1317	the observed effects	1298:1317	the observed effects	1298:1317	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	8	3	dep	indicated	1579:1587	arg1	as					1576:1577	as	1576:1577	as	1576:1577	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	3	4	theme	microbial	512:520	arg1	composition					522:532	the altered microbial composition	500:532	the altered microbial composition	500:532	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	5	5	from	reduction	894:902	arg1	inflammation					907:918	inflammation	907:918	inflammation	907:918	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	5	6	theme	mesenteric	1081:1090	arg1	nodes					1098:1102	the mesenteric lymph nodes	1077:1102	the mesenteric lymph nodes (MLN)	1077:1108	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	5	6	theme	mesenteric	1081:1090	arg1	MLN					1105:1107	MLN	1105:1107	MLN	1105:1107	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	3	7	theme	inflammatory	635:646	arg1	state					648:652	an inflammatory state	632:652	an inflammatory state	632:652	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	1	8	theme	ulcerative	170:179	arg1	colitis					181:187	ulcerative colitis	170:187	ulcerative colitis (UC)	170:192	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	8	theme	ulcerative	170:179	arg1	UC					190:191	UC	190:191	UC	190:191	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	9	theme	inflammatory	241:252	arg1	colitis					181:187	ulcerative colitis	170:187	ulcerative colitis (UC)	170:192	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	9	theme	inflammatory	241:252	arg1	disorders					254:262	chronic relapsing inflammatory disorders	223:262	chronic relapsing inflammatory disorders of the gastrointestinal tract	223:292	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	9	theme	inflammatory	241:252	arg1	diseases					145:152	Inflammatory bowel diseases	126:152	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD)	126:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	9	theme	inflammatory	241:252	arg1	disease					206:212	Crohn's disease	198:212	Crohn's disease (CD)	198:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	5	10	from	increase	1051:1058	arg1	cytokines					1005:1013	inflammatory cytokines	992:1013	inflammatory cytokines	992:1013	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	5	10	from	increase	1051:1058	arg1	cells					1068:1072	Treg cells	1063:1072	Treg cells in the mesenteric lymph nodes (MLN)	1063:1108	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	5	10	from	increase	1051:1058	arg1	IL-10					1028:1032	IL-10	1028:1032	IL-10	1028:1032	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	4	11	theme	intestinal	808:817	arg1	inflammation					819:830	IBD-like intestinal inflammation	799:830	IBD-like intestinal inflammation	799:830	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	8	12	dep	Optimizing	1419:1428	arg1	know					1486:1489	know	1486:1489	know to modulate the gut microbiota composition	1486:1532	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	4	13	theme	diet	781:784	arg1	content					760:766	the fibre content	750:766	the fibre content of a healthy diet	750:784	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	6	14	from	percentage	1172:1181	arg1	MLN					1256:1258	the MLN	1252:1258	the MLN	1252:1258	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	3	15	theme	regulatory	556:565	arg1	cells					567:571	T regulatory cells	554:571	T regulatory cells (Treg)	554:578	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	3	15	theme	regulatory	556:565	arg1	Treg					574:577	Treg	574:577	Treg	574:577	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	8	16	theme	present	1596:1602	arg1	study					1604:1608	the present study	1592:1608	the present study	1592:1608	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	3	17	theme	T	584:584	arg1	Th					600:601	Th	600:601	Th	600:601	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	3	17	theme	T	584:584	arg1	cells					593:597	T helper cells	584:597	T helper cells (Th)	584:602	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	1	18	theme	bowel	139:143	arg1	colitis					181:187	ulcerative colitis	170:187	ulcerative colitis (UC)	170:192	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	18	theme	bowel	139:143	arg1	IBD					155:157	IBD	155:157	IBD	155:157	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	18	theme	bowel	139:143	arg1	disorders					254:262	chronic relapsing inflammatory disorders	223:262	chronic relapsing inflammatory disorders of the gastrointestinal tract	223:292	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	18	theme	bowel	139:143	arg1	diseases					145:152	Inflammatory bowel diseases	126:152	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD)	126:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	18	theme	bowel	139:143	arg1	disease					206:212	Crohn's disease	198:212	Crohn's disease (CD)	198:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	4	19	theme	sulphate	866:873	arg1	mice					883:886	dextran sodium sulphate treated mice	851:886	dextran sodium sulphate treated mice	851:886	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	4	20	from	loss	843:846	arg1	mice					883:886	dextran sodium sulphate treated mice	851:886	dextran sodium sulphate treated mice	851:886	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	5	21	theme	inflammatory	992:1003	arg1	cytokines					1005:1013	inflammatory cytokines	992:1013	inflammatory cytokines	992:1013	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	0	22	from	role	12:15	arg1	amelioration					47:58	the amelioration	43:58	the amelioration of DSS	43:65	A potential role for regulatory T-cells in the amelioration of DSS induced colitis by dietary non-digestible polysaccharides.
25498760	0	23	theme	DSS	63:65	arg1	amelioration					47:58	the amelioration	43:58	the amelioration of DSS	43:65	A potential role for regulatory T-cells in the amelioration of DSS induced colitis by dietary non-digestible polysaccharides.
25498760	6	24	from	MLN	1256:1258	arg1	percentage					1172:1181	the percentage	1168:1181	the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN	1168:1258	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	6	25	theme	derived	1213:1219	arg1	cells					1243:1247	tolerogenic lamina propria derived CD103+RALDH+dendritic cells	1186:1247	tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN	1186:1258	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	6	26	dep	derived	1213:1219	arg1	propria					1205:1211	tolerogenic lamina propria	1186:1211	tolerogenic lamina propria	1186:1211	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	7	27	theme	used	1386:1389	arg1	nutrition					1358:1366	exclusive enteral nutrition	1340:1366	exclusive enteral nutrition (EEN)	1340:1372	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	7	27	theme	used	1386:1389	arg1	therapy					1410:1416	a widely used safe and effective therapy	1377:1416	a widely used safe and effective therapy	1377:1416	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	4	28	theme	dextran	851:857	arg1	sulphate					866:873	dextran sodium sulphate	851:873	dextran sodium sulphate treated mice	851:886	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	8	29	theme	SCFA	1535:1538	arg1	production					1540:1549	SCFA production	1535:1549	SCFA production	1535:1549	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	1	30	theme	relapsing	231:239	arg1	colitis					181:187	ulcerative colitis	170:187	ulcerative colitis (UC)	170:192	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	30	theme	relapsing	231:239	arg1	disorders					254:262	chronic relapsing inflammatory disorders	223:262	chronic relapsing inflammatory disorders of the gastrointestinal tract	223:292	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	30	theme	relapsing	231:239	arg1	diseases					145:152	Inflammatory bowel diseases	126:152	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD)	126:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	30	theme	relapsing	231:239	arg1	disease					206:212	Crohn's disease	198:212	Crohn's disease (CD)	198:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	2	31	theme	mucosal	369:375	arg1	barrier					377:383	the intestinal mucosal barrier	354:383	the intestinal mucosal barrier	354:383	The interaction between a disturbed microbial composition, the intestinal mucosal barrier and the mucosal immune system plays an important role in IBD and its chronicity.
25498760	5	32	theme	relative	1042:1049	arg1	increase					1051:1058	the relative increase	1038:1058	the relative increase in Treg cells in the mesenteric lymph nodes (MLN)	1038:1108	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	6	33	link	derived	1213:1219	arg1	cells					1243:1247	tolerogenic lamina propria derived CD103+RALDH+dendritic cells	1186:1247	tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN	1186:1258	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	2	34	theme	mucosal	393:399	arg1	system					408:413	the mucosal immune system	389:413	the mucosal immune system	389:413	The interaction between a disturbed microbial composition, the intestinal mucosal barrier and the mucosal immune system plays an important role in IBD and its chronicity.
25498760	0	35	theme	regulatory	21:30	arg1	T-cells					32:38	regulatory T-cells	21:38	regulatory T-cells	21:38	A potential role for regulatory T-cells in the amelioration of DSS induced colitis by dietary non-digestible polysaccharides.
25498760	1	36	theme	gastrointestinal	271:286	arg1	tract					288:292	the gastrointestinal tract	267:292	the gastrointestinal tract	267:292	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	5	37	from	increase	1016:1023	arg1	cytokines					1005:1013	inflammatory cytokines	992:1013	inflammatory cytokines	992:1013	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	5	37	from	increase	1016:1023	arg1	cells					1068:1072	Treg cells	1063:1072	Treg cells in the mesenteric lymph nodes (MLN)	1063:1108	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	5	37	from	increase	1016:1023	arg1	IL-10					1028:1032	IL-10	1028:1032	IL-10	1028:1032	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	2	38	theme	disturbed	321:329	arg1	composition					341:351	a disturbed microbial composition	319:351	a disturbed microbial composition	319:351	The interaction between a disturbed microbial composition, the intestinal mucosal barrier and the mucosal immune system plays an important role in IBD and its chronicity.
25498760	7	39	from	therapy	1410:1416	arg1	children					1323:1330	children	1323:1330	children with CD	1323:1338	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	4	40	theme	multi	710:714	arg1	MF					727:728	MF	727:728	MF	727:728	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	4	40	theme	multi	710:714	arg1	mix					722:724	a specific multi fibre mix	699:724	a specific multi fibre mix (MF)	699:729	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	5	41	theme	lymph	1092:1096	arg1	nodes					1098:1102	the mesenteric lymph nodes	1077:1102	the mesenteric lymph nodes (MLN)	1077:1108	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	5	41	theme	lymph	1092:1096	arg1	MLN					1105:1107	MLN	1105:1107	MLN	1105:1107	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	7	42	theme	exclusive	1340:1348	arg1	nutrition					1358:1366	exclusive enteral nutrition	1340:1366	exclusive enteral nutrition (EEN)	1340:1372	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	7	42	theme	exclusive	1340:1348	arg1	therapy					1410:1416	a widely used safe and effective therapy	1377:1416	a widely used safe and effective therapy	1377:1416	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	7	42	theme	exclusive	1340:1348	arg1	EEN					1369:1371	EEN	1369:1371	EEN	1369:1371	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	8	43	theme	enteral	1430:1436	arg1	concepts					1450:1457	enteral nutritional concepts	1430:1457	enteral nutritional concepts	1430:1457	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	4	44	theme	mix	722:724	arg1	intake					689:694	oral intake	684:694	oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet,	684:785	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	7	45	with	children	1323:1330	arg1	CD					1337:1338	CD	1337:1338	CD	1337:1338	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	4	46	theme	fibre	754:758	arg1	content					760:766	the fibre content	750:766	the fibre content of a healthy diet	750:784	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	6	47	theme	Treg	1125:1128	arg1	percentage					1130:1139	the Treg percentage	1121:1139	the Treg percentage in the MLN	1121:1150	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	3	48	theme	altered	504:510	arg1	composition					522:532	the altered microbial composition	500:532	the altered microbial composition	500:532	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	8	49	theme	microbiota	1511:1520	arg1	composition					1522:1532	the gut microbiota composition	1503:1532	the gut microbiota composition	1503:1532	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	1	50	theme	chronic	223:229	arg1	colitis					181:187	ulcerative colitis	170:187	ulcerative colitis (UC)	170:192	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	50	theme	chronic	223:229	arg1	disorders					254:262	chronic relapsing inflammatory disorders	223:262	chronic relapsing inflammatory disorders of the gastrointestinal tract	223:292	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	50	theme	chronic	223:229	arg1	diseases					145:152	Inflammatory bowel diseases	126:152	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD)	126:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	50	theme	chronic	223:229	arg1	disease					206:212	Crohn's disease	198:212	Crohn's disease (CD)	198:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	8	51	theme	fibre	1475:1479	arg1	mix					1481:1483	the tested fibre mix	1464:1483	the tested fibre mix	1464:1483	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	6	52	theme	lamina	1198:1203	arg1	propria					1205:1211	tolerogenic lamina propria	1186:1211	tolerogenic lamina propria	1186:1211	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	6	53	theme	cells	1243:1247	arg1	percentage					1172:1181	the percentage	1168:1181	the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN	1168:1258	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	3	54	dep	cells	567:571	arg1	17					604:605	17	604:605	17	604:605	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	6	55	theme	tolerogenic	1186:1196	arg1	propria					1205:1211	tolerogenic lamina propria	1186:1211	tolerogenic lamina propria	1186:1211	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	8	56	theme	inflammatory	1555:1566	arg1	status					1568:1573	inflammatory status	1555:1573	inflammatory status (as indicated by the present study)	1555:1609	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	4	57	theme	IBD-like	799:806	arg1	inflammation					819:830	IBD-like intestinal inflammation	799:830	IBD-like intestinal inflammation	799:830	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	5	58	from	cells	1068:1072	arg1	nodes					1098:1102	the mesenteric lymph nodes	1077:1102	the mesenteric lymph nodes (MLN)	1077:1108	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	5	58	from	cells	1068:1072	arg1	MLN					1105:1107	MLN	1105:1107	MLN	1105:1107	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	4	59	from	inflammation	819:830	arg1	mice					883:886	dextran sodium sulphate treated mice	851:886	dextran sodium sulphate treated mice	851:886	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	3	60	theme	T	554:554	arg1	cells					567:571	T regulatory cells	554:571	T regulatory cells (Treg)	554:578	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	3	60	theme	T	554:554	arg1	Treg					574:577	Treg	574:577	Treg	574:577	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	4	61	theme	healthy	773:779	arg1	diet					781:784	a healthy diet	771:784	a healthy diet	771:784	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	1	62	theme	Inflammatory	126:137	arg1	colitis					181:187	ulcerative colitis	170:187	ulcerative colitis (UC)	170:192	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	62	theme	Inflammatory	126:137	arg1	IBD					155:157	IBD	155:157	IBD	155:157	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	62	theme	Inflammatory	126:137	arg1	disorders					254:262	chronic relapsing inflammatory disorders	223:262	chronic relapsing inflammatory disorders of the gastrointestinal tract	223:292	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	62	theme	Inflammatory	126:137	arg1	diseases					145:152	Inflammatory bowel diseases	126:152	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD)	126:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	62	theme	Inflammatory	126:137	arg1	disease					206:212	Crohn's disease	198:212	Crohn's disease (CD)	198:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	7	63	theme	effective	1400:1408	arg1	nutrition					1358:1366	exclusive enteral nutrition	1340:1366	exclusive enteral nutrition (EEN)	1340:1372	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	7	63	theme	effective	1400:1408	arg1	therapy					1410:1416	a widely used safe and effective therapy	1377:1416	a widely used safe and effective therapy	1377:1416	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	4	64	theme	sodium	859:864	arg1	sulphate					866:873	dextran sodium sulphate	851:873	dextran sodium sulphate treated mice	851:886	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	6	65	from	cells	1243:1247	arg1	MLN					1256:1258	the MLN	1252:1258	the MLN	1252:1258	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	7	66	theme	safe	1391:1394	arg1	nutrition					1358:1366	exclusive enteral nutrition	1340:1366	exclusive enteral nutrition (EEN)	1340:1372	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	7	66	theme	safe	1391:1394	arg1	therapy					1410:1416	a widely used safe and effective therapy	1377:1416	a widely used safe and effective therapy	1377:1416	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	4	67	theme	treated	875:881	arg1	mice					883:886	dextran sodium sulphate treated mice	851:886	dextran sodium sulphate treated mice	851:886	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	6	68	theme	CD103+RALDH+dendritic	1221:1241	arg1	cells					1243:1247	tolerogenic lamina propria derived CD103+RALDH+dendritic cells	1186:1247	tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN	1186:1258	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
25498760	4	69	theme	weight	836:841	arg1	loss					843:846	weight loss	836:846	weight loss	836:846	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	8	70	dep	status	1568:1573	arg1	indicated					1579:1587	indicated	1579:1587	indicated by the present study	1579:1608	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	4	71	theme	oral	684:687	arg1	intake					689:694	oral intake	684:694	oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet,	684:785	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	0	72	theme	non-digestible	94:107	arg1	polysaccharides					109:123	dietary non-digestible polysaccharides	86:123	dietary non-digestible polysaccharides	86:123	A potential role for regulatory T-cells in the amelioration of DSS induced colitis by dietary non-digestible polysaccharides.
25498760	5	73	theme	MF-induced	969:978	arg1	decrease					980:987	the MF-induced decrease	965:987	the MF-induced decrease in inflammatory cytokines	965:1013	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	0	74	theme	potential	2:10	arg1	role					12:15	A potential role	0:15	A potential role for regulatory T-cells in the amelioration of DSS	0:65	A potential role for regulatory T-cells in the amelioration of DSS induced colitis by dietary non-digestible polysaccharides.
25498760	2	75	theme	important	424:432	arg1	role					434:437	an important role	421:437	an important role	421:437	The interaction between a disturbed microbial composition, the intestinal mucosal barrier and the mucosal immune system plays an important role in IBD and its chronicity.
25498760	4	76	theme	present	659:665	arg1	study					667:671	The present study	655:671	The present study	655:671	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	2	77	theme	intestinal	358:367	arg1	barrier					377:383	the intestinal mucosal barrier	354:383	the intestinal mucosal barrier	354:383	The interaction between a disturbed microbial composition, the intestinal mucosal barrier and the mucosal immune system plays an important role in IBD and its chronicity.
25498760	2	78	theme	immune	401:406	arg1	system					408:413	the mucosal immune system	389:413	the mucosal immune system	389:413	The interaction between a disturbed microbial composition, the intestinal mucosal barrier and the mucosal immune system plays an important role in IBD and its chronicity.
25498760	5	79	theme	Treg	1063:1066	arg1	cells					1068:1072	Treg cells	1063:1072	Treg cells in the mesenteric lymph nodes (MLN)	1063:1108	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	3	80	theme	helper	586:591	arg1	Th					600:601	Th	600:601	Th	600:601	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	3	80	theme	helper	586:591	arg1	cells					593:597	T helper cells	584:597	T helper cells (Th)	584:602	It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state.
25498760	0	81	theme	dietary	86:92	arg1	polysaccharides					109:123	dietary non-digestible polysaccharides	86:123	dietary non-digestible polysaccharides	86:123	A potential role for regulatory T-cells in the amelioration of DSS induced colitis by dietary non-digestible polysaccharides.
25498760	1	82	theme	tract	288:292	arg1	colitis					181:187	ulcerative colitis	170:187	ulcerative colitis (UC)	170:192	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	82	theme	tract	288:292	arg1	disorders					254:262	chronic relapsing inflammatory disorders	223:262	chronic relapsing inflammatory disorders of the gastrointestinal tract	223:292	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	82	theme	tract	288:292	arg1	diseases					145:152	Inflammatory bowel diseases	126:152	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD)	126:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	1	82	theme	tract	288:292	arg1	disease					206:212	Crohn's disease	198:212	Crohn's disease (CD)	198:217	Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract.
25498760	8	83	theme	nutritional	1438:1448	arg1	concepts					1450:1457	enteral nutritional concepts	1430:1457	enteral nutritional concepts	1430:1457	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	4	84	theme	specific	701:708	arg1	MF					727:728	MF	727:728	MF	727:728	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	4	84	theme	specific	701:708	arg1	mix					722:724	a specific multi fibre mix	699:724	a specific multi fibre mix (MF)	699:729	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	7	85	theme	enteral	1350:1356	arg1	nutrition					1358:1366	exclusive enteral nutrition	1340:1366	exclusive enteral nutrition (EEN)	1340:1372	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	7	85	theme	enteral	1350:1356	arg1	therapy					1410:1416	a widely used safe and effective therapy	1377:1416	a widely used safe and effective therapy	1377:1416	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	7	85	theme	enteral	1350:1356	arg1	EEN					1369:1371	EEN	1369:1371	EEN	1369:1371	In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy.
25498760	4	86	theme	fibre	716:720	arg1	MF					727:728	MF	727:728	MF	727:728	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	4	86	theme	fibre	716:720	arg1	mix					722:724	a specific multi fibre mix	699:724	a specific multi fibre mix (MF)	699:729	The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice.
25498760	5	87	from	decrease	980:987	arg1	cytokines					1005:1013	inflammatory cytokines	992:1013	inflammatory cytokines	992:1013	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	5	87	from	decrease	980:987	arg1	cells					1068:1072	Treg cells	1063:1072	Treg cells in the mesenteric lymph nodes (MLN)	1063:1108	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	5	87	from	decrease	980:987	arg1	IL-10					1028:1032	IL-10	1028:1032	IL-10	1028:1032	This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN).
25498760	8	88	theme	gut	1507:1509	arg1	composition					1522:1532	the gut microbiota composition	1503:1532	the gut microbiota composition	1503:1532	Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
25498760	6	89	from	percentage	1130:1139	arg1	MLN					1148:1150	the MLN	1144:1150	the MLN	1144:1150	Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects.
27267934	7	0	theme	%	1218:1218	arg1	B-GOS					1220:1224	The 65 % B-GOS	1211:1224	The 65 % B-GOS	1211:1224	The 65 % B-GOS showed positive modulation of the microbiota composition during the first 8 h of fermentation with all doses.
27267934	6	1	dep	in	1069:1070	arg1	situ					1072:1075	situ	1072:1075	situ	1072:1075	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	6	2	theme	1H-NMR	1173:1178	arg1	analysis					1180:1187	1H-NMR analysis	1173:1187	1H-NMR analysis	1173:1187	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	4	3	theme	B-GOS	768:772	arg1	Fermentation					747:758	Fermentation	747:758	Fermentation of 65 % B-GOS	747:772	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	1	4	theme	health	294:299	arg1	benefits					301:308	health benefits	294:308	health benefits	294:308	Prebiotic oligosaccharides have the ability to generate important changes in the gut microbiota composition that may confer health benefits to the host.
27267934	10	5	theme	such	1698:1701	arg1	changes					1703:1709	such changes	1698:1709	such changes	1698:1709	However, the applicability of such changes remains to be shown in an in vivo trial.
27267934	9	6	theme	prebiotic	1650:1658	arg1	effect					1660:1665	an efficient prebiotic effect	1637:1665	an efficient prebiotic effect	1637:1665	The 65 % B-GOS in syrup format seems to have, in all the analysis, an efficient prebiotic effect.
27267934	2	7	from	applications	388:399	arg1	industries					409:418	food industries	404:418	food industries	404:418	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	0	8	theme	in	134:135	arg1	parameters					158:167	in vitro gut microbiota parameters	134:167	in vitro gut microbiota parameters	134:167	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	3	9	theme	fermentation	602:613	arg1	properties					615:624	the in vitro potential fermentation properties	579:624	the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites	579:744	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	1	10	theme	important	226:234	arg1	changes					236:242	important changes	226:242	important changes	226:242	Prebiotic oligosaccharides have the ability to generate important changes in the gut microbiota composition that may confer health benefits to the host.
27267934	10	11	theme	in	1737:1738	arg1	trial					1745:1749	an in vivo trial	1734:1749	an in vivo trial	1734:1749	However, the applicability of such changes remains to be shown in an in vivo trial.
27267934	0	12	theme	gut	143:145	arg1	parameters					158:167	in vitro gut microbiota parameters	134:167	in vitro gut microbiota parameters	134:167	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	3	13	theme	galacto-oligosaccharide	636:658	arg1	B-GOS					687:691	B-GOS	687:691	B-GOS	687:691	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	3	13	theme	galacto-oligosaccharide	636:658	arg1	GOS					682:684	a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS	629:684	a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS)	629:692	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	3	14	theme	65	631:632	arg1	%					634:634	%	634:634	%	634:634	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	5	15	theme	0·33	927:930	arg1	g					932:932	1, 0·5 and 0·33 g	916:932	g	932:932	In total, three different doses (1, 0·5 and 0·33 g equivalent to 0·1, 0·05 and 0·033 g/l) were tested.
27267934	4	16	theme	65	763:764	arg1	%					766:766	%	766:766	%	766:766	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	3	17	theme	content	666:672	arg1	B-GOS					687:691	B-GOS	687:691	B-GOS	687:691	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	3	17	theme	content	666:672	arg1	GOS					682:684	a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS	629:684	a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS)	629:692	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	5	18	theme	equivalent	934:943	arg1	g					932:932	1, 0·5 and 0·33 g	916:932	g	932:932	In total, three different doses (1, 0·5 and 0·33 g equivalent to 0·1, 0·05 and 0·033 g/l) were tested.
27267934	6	19	theme	gut	1001:1003	arg1	microbiota					1005:1014	the gut microbiota	997:1014	the gut microbiota	997:1014	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	7	20	theme	65	1215:1216	arg1	%					1218:1218	%	1218:1218	%	1218:1218	The 65 % B-GOS showed positive modulation of the microbiota composition during the first 8 h of fermentation with all doses.
27267934	3	21	theme	GOS	661:663	arg1	B-GOS					687:691	B-GOS	687:691	B-GOS	687:691	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	3	21	theme	GOS	661:663	arg1	GOS					682:684	a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS	629:684	a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS)	629:692	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	5	22	theme	1	916:916	arg1	g					932:932	1, 0·5 and 0·33 g	916:932	g	932:932	In total, three different doses (1, 0·5 and 0·33 g equivalent to 0·1, 0·05 and 0·033 g/l) were tested.
27267934	2	23	from	effect	464:469	arg1	lactobacilli					535:546	lactobacilli	535:546	lactobacilli	535:546	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	2	23	from	effect	464:469	arg1	bacteria					499:506	the potential beneficial bacteria	474:506	the potential beneficial bacteria such as bifidobacteria and lactobacilli	474:546	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	2	23	from	effect	464:469	arg1	bifidobacteria					516:529	bifidobacteria	516:529	bifidobacteria	516:529	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	0	24	theme	65	93:94	arg1	%					96:96	%	96:96	%	96:96	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	4	25	from	B-GOS	797:801	arg1	experiments					870:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	0	26	dep	in	134:135	arg1	vitro					137:141	vitro	137:141	vitro	137:141	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	2	27	from	impurities	336:345	arg1	mixtures					360:367	prebiotic mixtures	350:367	prebiotic mixtures	350:367	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	4	28	theme	52	792:793	arg1	%					795:795	%	795:795	%	795:795	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	0	29	theme	galacto-oligosaccharide	98:120	arg1	content					122:128	65 % galacto-oligosaccharide content	93:128	65 % galacto-oligosaccharide content	93:128	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	0	29	theme	galacto-oligosaccharide	98:120	arg1	galacto-oligosaccharide					68:90	Bimuno® galacto-oligosaccharide	60:90	Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content)	60:129	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	7	30	theme	first	1294:1298	arg1	h					1302:1302	the first 8 h	1290:1302	the first 8 h of fermentation	1290:1318	The 65 % B-GOS showed positive modulation of the microbiota composition during the first 8 h of fermentation with all doses.
27267934	8	31	dep	propionate	1468:1477	arg1	concentrations					1492:1505	concentrations	1492:1505	concentrations	1492:1505	Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates.
27267934	7	32	theme	fermentation	1307:1318	arg1	h					1302:1302	the first 8 h	1290:1302	the first 8 h of fermentation	1290:1318	The 65 % B-GOS showed positive modulation of the microbiota composition during the first 8 h of fermentation with all doses.
27267934	2	33	theme	potential	478:486	arg1	lactobacilli					535:546	lactobacilli	535:546	lactobacilli	535:546	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	2	33	theme	potential	478:486	arg1	bacteria					499:506	the potential beneficial bacteria	474:506	the potential beneficial bacteria such as bifidobacteria and lactobacilli	474:546	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	2	33	theme	potential	478:486	arg1	bifidobacteria					516:529	bifidobacteria	516:529	bifidobacteria	516:529	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	6	34	from	Changes	986:992	arg1	microbiota					1005:1014	the gut microbiota	997:1014	the gut microbiota	997:1014	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	6	35	dep	identified	1042:1051	arg1	whereas					1092:1098	whereas	1092:1098	whereas	1092:1098	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	0	36	theme	Fermentation	0:11	arg1	properties					13:22	Fermentation properties	0:22	Fermentation properties	0:22	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	8	37	theme	specific	1358:1365	arg1	doses					1367:1371	the specific doses	1354:1371	the specific doses of B-GOS	1354:1380	Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates.
27267934	2	38	theme	prebiotic	454:462	arg1	effect					464:469	prebiotic effect	454:469	prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli	454:546	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	8	39	theme	doses	1367:1371	arg1	Administration					1336:1349	Administration	1336:1349	Administration of the specific doses of B-GOS	1336:1380	Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates.
27267934	9	40	theme	65	1574:1575	arg1	%					1577:1577	%	1577:1577	%	1577:1577	The 65 % B-GOS in syrup format seems to have, in all the analysis, an efficient prebiotic effect.
27267934	6	41	theme	time	1025:1028	arg1	course					1030:1035	a time course	1023:1035	a time course	1023:1035	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	0	42	theme	galacto-oligosaccharide	68:90	arg1	activity					48:55	potential prebiotic activity	28:55	potential prebiotic activity	28:55	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	0	42	theme	galacto-oligosaccharide	68:90	arg1	properties					13:22	Fermentation properties	0:22	Fermentation properties	0:22	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	4	43	theme	anaerobic	846:854	arg1	experiments					870:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	0	44	theme	prebiotic	38:46	arg1	activity					48:55	potential prebiotic activity	28:55	potential prebiotic activity	28:55	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	3	45	theme	GOS	682:684	arg1	properties					615:624	the in vitro potential fermentation properties	579:624	the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites	579:744	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	4	46	theme	culture	862:868	arg1	experiments					870:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	9	47	theme	syrup	1588:1592	arg1	format					1594:1599	syrup format	1588:1599	syrup format	1588:1599	The 65 % B-GOS in syrup format seems to have, in all the analysis, an efficient prebiotic effect.
27267934	6	48	theme	molecular	1106:1114	arg1	profiles					1136:1143	small molecular weight metabolomics profiles	1100:1143	small molecular weight metabolomics profiles	1100:1143	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	3	49	theme	microbiota	701:710	arg1	composition					712:722	gut microbiota composition	697:722	gut microbiota composition	697:722	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	8	50	theme	significant	1392:1402	arg1	increase					1404:1411	a significant increase	1390:1411	a significant increase in acetate	1390:1422	Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates.
27267934	8	50	theme	significant	1392:1402	arg1	SCFA					1437:1440	the major SCFA	1427:1440	the major SCFA synthesised compared with propionate and butyrate concentrations	1427:1505	Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates.
27267934	4	51	theme	dose-response	832:844	arg1	experiments					870:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	2	52	theme	prebiotic	350:358	arg1	mixtures					360:367	prebiotic mixtures	350:367	prebiotic mixtures	350:367	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	2	53	from	selectivity	438:448	arg1	lactobacilli					535:546	lactobacilli	535:546	lactobacilli	535:546	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	2	53	from	selectivity	438:448	arg1	bacteria					499:506	the potential beneficial bacteria	474:506	the potential beneficial bacteria such as bifidobacteria and lactobacilli	474:546	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	2	53	from	selectivity	438:448	arg1	bifidobacteria					516:529	bifidobacteria	516:529	bifidobacteria	516:529	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	4	54	theme	volume-controlled	814:830	arg1	experiments					870:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	1	55	theme	microbiota	255:264	arg1	composition					266:276	the gut microbiota composition	247:276	the gut microbiota composition that may confer health benefits to the host	247:320	Prebiotic oligosaccharides have the ability to generate important changes in the gut microbiota composition that may confer health benefits to the host.
27267934	7	56	theme	positive	1233:1240	arg1	modulation					1242:1251	positive modulation	1233:1251	positive modulation of the microbiota composition	1233:1281	The 65 % B-GOS showed positive modulation of the microbiota composition during the first 8 h of fermentation with all doses.
27267934	6	57	theme	in	1069:1070	arg1	hybridisation					1077:1089	fluorescence in situ hybridisation	1056:1089	fluorescence in situ hybridisation	1056:1089	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	3	58	theme	in	583:584	arg1	properties					615:624	the in vitro potential fermentation properties	579:624	the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites	579:744	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	1	59	contain	have	197:200	arg2	ability					206:212	the ability to generate important changes	202:242	the ability to generate important changes	202:242	Prebiotic oligosaccharides have the ability to generate important changes in the gut microbiota composition that may confer health benefits to the host.
27267934	1	59	contain	have	197:200	arg1	oligosaccharides					180:195	Prebiotic oligosaccharides	170:195	Prebiotic oligosaccharides	170:195	Prebiotic oligosaccharides have the ability to generate important changes in the gut microbiota composition that may confer health benefits to the host.
27267934	10	60	theme	changes	1703:1709	arg1	applicability					1681:1693	the applicability	1677:1693	the applicability of such changes	1677:1709	However, the applicability of such changes remains to be shown in an in vivo trial.
27267934	5	61	theme	different	899:907	arg1	doses					909:913	three different doses	893:913	three different doses (1, 0·5 and 0·33 g equivalent to 0·1, 0·05 and 0·033 g/l)	893:971	In total, three different doses (1, 0·5 and 0·33 g equivalent to 0·1, 0·05 and 0·033 g/l) were tested.
27267934	9	62	theme	efficient	1640:1648	arg1	effect					1660:1665	an efficient prebiotic effect	1637:1665	an efficient prebiotic effect	1637:1665	The 65 % B-GOS in syrup format seems to have, in all the analysis, an efficient prebiotic effect.
27267934	8	63	theme	B-GOS	1376:1380	arg1	doses					1367:1371	the specific doses	1354:1371	the specific doses of B-GOS	1354:1380	Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates.
27267934	3	64	theme	potential	592:600	arg1	properties					615:624	the in vitro potential fermentation properties	579:624	the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites	579:744	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	8	65	from	increase	1404:1411	arg1	acetate					1416:1422	acetate	1416:1422	acetate	1416:1422	Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates.
27267934	6	66	theme	metabolomics	1123:1134	arg1	profiles					1136:1143	small molecular weight metabolomics profiles	1100:1143	small molecular weight metabolomics profiles	1100:1143	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	10	67	dep	in	1737:1738	arg1	vivo					1740:1743	vivo	1740:1743	vivo	1740:1743	However, the applicability of such changes remains to be shown in an in vivo trial.
27267934	9	68	contain	have	1610:1613	arg1	B-GOS					1579:1583	The 65 % B-GOS	1570:1583	The 65 % B-GOS in syrup format	1570:1599	The 65 % B-GOS in syrup format seems to have, in all the analysis, an efficient prebiotic effect.
27267934	9	68	contain	have	1610:1613	arg2	effect					1660:1665	an efficient prebiotic effect	1637:1665	an efficient prebiotic effect	1637:1665	The 65 % B-GOS in syrup format seems to have, in all the analysis, an efficient prebiotic effect.
27267934	7	69	theme	microbiota	1260:1269	arg1	composition					1271:1281	the microbiota composition	1256:1281	the microbiota composition	1256:1281	The 65 % B-GOS showed positive modulation of the microbiota composition during the first 8 h of fermentation with all doses.
27267934	0	70	theme	microbiota	147:156	arg1	parameters					158:167	in vitro gut microbiota parameters	134:167	in vitro gut microbiota parameters	134:167	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	3	71	theme	%	634:634	arg1	B-GOS					687:691	B-GOS	687:691	B-GOS	687:691	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	3	71	theme	%	634:634	arg1	GOS					682:684	a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS	629:684	a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS)	629:692	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	8	72	theme	major	1431:1435	arg1	increase					1404:1411	a significant increase	1390:1411	a significant increase in acetate	1390:1422	Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates.
27267934	8	72	theme	major	1431:1435	arg1	SCFA					1437:1440	the major SCFA	1427:1440	the major SCFA synthesised compared with propionate and butyrate concentrations	1427:1505	Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates.
27267934	4	73	theme	pH-	806:808	arg1	experiments					870:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	3	74	dep	in	583:584	arg1	vitro					586:590	vitro	586:590	vitro	586:590	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	3	75	from	properties	615:624	arg1	composition					712:722	gut microbiota composition	697:722	gut microbiota composition	697:722	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	3	75	from	properties	615:624	arg1	metabolites					734:744	their metabolites	728:744	their metabolites	728:744	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	0	76	theme	%	96:96	arg1	content					122:128	65 % galacto-oligosaccharide content	93:128	65 % galacto-oligosaccharide content	93:128	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	0	76	theme	%	96:96	arg1	galacto-oligosaccharide					68:90	Bimuno® galacto-oligosaccharide	60:90	Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content)	60:129	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	1	77	theme	Prebiotic	170:178	arg1	oligosaccharides					180:195	Prebiotic oligosaccharides	170:195	Prebiotic oligosaccharides	170:195	Prebiotic oligosaccharides have the ability to generate important changes in the gut microbiota composition that may confer health benefits to the host.
27267934	0	78	from	properties	13:22	arg1	parameters					158:167	in vitro gut microbiota parameters	134:167	in vitro gut microbiota parameters	134:167	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	6	79	theme	fluorescence	1056:1067	arg1	hybridisation					1077:1089	fluorescence in situ hybridisation	1056:1089	fluorescence in situ hybridisation	1056:1089	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	0	80	from	activity	48:55	arg1	parameters					158:167	in vitro gut microbiota parameters	134:167	in vitro gut microbiota parameters	134:167	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	4	81	theme	%	795:795	arg1	B-GOS					797:801	52 % B-GOS	792:801	52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments	792:880	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	8	82	theme	significant	1526:1536	arg1	differences					1538:1548	no significant differences	1523:1548	no significant differences between substrates	1523:1567	Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates.
27267934	4	83	theme	%	766:766	arg1	B-GOS					768:772	65 % B-GOS	763:772	65 % B-GOS	763:772	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	2	84	theme	food	404:407	arg1	industries					409:418	food industries	404:418	food industries	404:418	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	0	85	theme	potential	28:36	arg1	activity					48:55	potential prebiotic activity	28:55	potential prebiotic activity	28:55	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	9	86	theme	%	1577:1577	arg1	B-GOS					1579:1583	The 65 % B-GOS	1570:1583	The 65 % B-GOS in syrup format	1570:1599	The 65 % B-GOS in syrup format seems to have, in all the analysis, an efficient prebiotic effect.
27267934	9	87	from	B-GOS	1579:1583	arg1	format					1594:1599	syrup format	1588:1599	syrup format	1588:1599	The 65 % B-GOS in syrup format seems to have, in all the analysis, an efficient prebiotic effect.
27267934	7	88	theme	composition	1271:1281	arg1	modulation					1242:1251	positive modulation	1233:1251	positive modulation of the microbiota composition	1233:1281	The 65 % B-GOS showed positive modulation of the microbiota composition during the first 8 h of fermentation with all doses.
27267934	3	89	theme	Bimuno®	674:680	arg1	B-GOS					687:691	B-GOS	687:691	B-GOS	687:691	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	3	89	theme	Bimuno®	674:680	arg1	GOS					682:684	a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS	629:684	a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS)	629:692	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	5	90	dep	doses	909:913	arg1	g					932:932	1, 0·5 and 0·33 g	916:932	g	932:932	In total, three different doses (1, 0·5 and 0·33 g equivalent to 0·1, 0·05 and 0·033 g/l) were tested.
27267934	0	91	theme	Bimuno®	60:66	arg1	content					122:128	65 % galacto-oligosaccharide content	93:128	65 % galacto-oligosaccharide content	93:128	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	0	91	theme	Bimuno®	60:66	arg1	galacto-oligosaccharide					68:90	Bimuno® galacto-oligosaccharide	60:90	Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content)	60:129	Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
27267934	6	92	theme	weight	1116:1121	arg1	profiles					1136:1143	small molecular weight metabolomics profiles	1100:1143	small molecular weight metabolomics profiles	1100:1143	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	2	93	theme	beneficial	488:497	arg1	lactobacilli					535:546	lactobacilli	535:546	lactobacilli	535:546	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	2	93	theme	beneficial	488:497	arg1	bacteria					499:506	the potential beneficial bacteria	474:506	the potential beneficial bacteria such as bifidobacteria and lactobacilli	474:546	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	2	93	theme	beneficial	488:497	arg1	bifidobacteria					516:529	bifidobacteria	516:529	bifidobacteria	516:529	Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli.
27267934	3	94	theme	gut	697:699	arg1	composition					712:722	gut microbiota composition	697:722	gut microbiota composition	697:722	This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites.
27267934	4	95	theme	batch	856:860	arg1	experiments					870:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	pH- and volume-controlled dose-response anaerobic batch culture experiments	806:880	Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments.
27267934	6	96	theme	small	1100:1104	arg1	profiles					1136:1143	small molecular weight metabolomics profiles	1100:1143	small molecular weight metabolomics profiles	1100:1143	Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively.
27267934	1	97	theme	gut	251:253	arg1	composition					266:276	the gut microbiota composition	247:276	the gut microbiota composition that may confer health benefits to the host	247:320	Prebiotic oligosaccharides have the ability to generate important changes in the gut microbiota composition that may confer health benefits to the host.
26698559	0	0	theme	wood	63:66	arg1	sawdust					68:74	alginate-Ayous wood sawdust	48:74	alginate-Ayous wood sawdust (Triplochiton scleroxylon)	48:101	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon) composite material.
26698559	0	0	theme	wood	63:66	arg1	scleroxylon					90:100	Triplochiton scleroxylon	77:100	Triplochiton scleroxylon	77:100	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon) composite material.
26698559	2	1	theme	experimental	434:445	arg1	data					447:450	The experimental data	430:450	The experimental data	430:450	The experimental data fitted well with the Langmuir isotherm, suggesting that monolayer adsorption of the cadmium ions onto alginate-Ayous sawdust composite (a-ASC).
26698559	0	2	theme	alginate-Ayous	48:61	arg1	sawdust					68:74	alginate-Ayous wood sawdust	48:74	alginate-Ayous wood sawdust (Triplochiton scleroxylon)	48:101	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon) composite material.
26698559	0	2	theme	alginate-Ayous	48:61	arg1	scleroxylon					90:100	Triplochiton scleroxylon	77:100	Triplochiton scleroxylon	77:100	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon) composite material.
26698559	3	3	theme	a-ASC	642:646	arg1	mg/g					669:672	6.21 mg/g	664:672	6.21 mg/g	664:672	The obtained monolayer adsorption capacity of a-ASC for Cd (II) was 6.21 mg/g.
26698559	3	3	theme	a-ASC	642:646	arg1	capacity					630:637	The obtained monolayer adsorption capacity	596:637	The obtained monolayer adsorption capacity of a-ASC for Cd (II)	596:658	The obtained monolayer adsorption capacity of a-ASC for Cd (II) was 6.21 mg/g.
26698559	1	4	from	characteristics	139:153	arg1	wastewater					186:195	synthetic wastewater	176:195	synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate	176:289	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	4	5	theme	Cd	798:799	arg1	biosorption					806:816	Cd (II) biosorption	798:816	Cd (II) biosorption	798:816	From the Dubinin-Radushkevich isotherm model, a 5.39 kJ/mol value for the mean free energy was calculated, indicating that Cd (II) biosorption could include an important physisorption stage.
26698559	2	6	theme	Langmuir	473:480	arg1	isotherm					482:489	the Langmuir isotherm	469:489	the Langmuir isotherm	469:489	The experimental data fitted well with the Langmuir isotherm, suggesting that monolayer adsorption of the cadmium ions onto alginate-Ayous sawdust composite (a-ASC).
26698559	2	7	theme	ions	544:547	arg1	adsorption					518:527	monolayer adsorption	508:527	monolayer adsorption of the cadmium ions onto alginate-Ayous	508:567	The experimental data fitted well with the Langmuir isotherm, suggesting that monolayer adsorption of the cadmium ions onto alginate-Ayous sawdust composite (a-ASC).
26698559	1	8	theme	metal	392:396	arg1	time					355:358	contact time	347:358	contact time	347:358	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	8	theme	metal	392:396	arg1	pH					325:326	pH	325:326	pH	325:326	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	8	theme	metal	392:396	arg1	temperature					399:409	temperature	399:409	temperature	399:409	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	8	theme	metal	392:396	arg1	concentration					369:381	initial concentration	361:381	initial concentration of heavy metal	361:396	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	8	theme	metal	392:396	arg1	quantity					337:344	biomass quantity	329:344	biomass quantity	329:344	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	8	theme	metal	392:396	arg1	rate					424:427	stirring rate	415:427	stirring rate	415:427	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	4	9	theme	isotherm	705:712	arg1	model					714:718	the Dubinin-Radushkevich isotherm model	680:718	the Dubinin-Radushkevich isotherm model	680:718	From the Dubinin-Radushkevich isotherm model, a 5.39 kJ/mol value for the mean free energy was calculated, indicating that Cd (II) biosorption could include an important physisorption stage.
26698559	1	10	theme	Triplochiton	227:238	arg1	sawdust					218:224	raw Ayous wood sawdust	203:224	raw Ayous wood sawdust (Triplochiton scleroxylon)	203:251	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	10	theme	Triplochiton	227:238	arg1	scleroxylon					240:250	Triplochiton scleroxylon	227:250	Triplochiton scleroxylon	227:250	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	2	11	theme	cadmium	536:542	arg1	ions					544:547	the cadmium ions	532:547	the cadmium ions	532:547	The experimental data fitted well with the Langmuir isotherm, suggesting that monolayer adsorption of the cadmium ions onto alginate-Ayous sawdust composite (a-ASC).
26698559	4	12	theme	Dubinin-Radushkevich	684:703	arg1	model					714:718	the Dubinin-Radushkevich isotherm model	680:718	the Dubinin-Radushkevich isotherm model	680:718	From the Dubinin-Radushkevich isotherm model, a 5.39 kJ/mol value for the mean free energy was calculated, indicating that Cd (II) biosorption could include an important physisorption stage.
26698559	5	13	from	nature	982:987	arg1	feasible					941:948	feasible	941:948	feasible	941:948	Thermodynamic calculations showed that the Cd (II) biosorption process was feasible, endothermic and spontaneous in nature under examined conditions.
26698559	5	13	from	nature	982:987	arg1	process					929:935	the Cd (II) biosorption process	905:935	the Cd (II) biosorption process	905:935	Thermodynamic calculations showed that the Cd (II) biosorption process was feasible, endothermic and spontaneous in nature under examined conditions.
26698559	3	14	theme	6.21	664:667	arg1	mg/g					669:672	6.21 mg/g	664:672	6.21 mg/g	664:672	The obtained monolayer adsorption capacity of a-ASC for Cd (II) was 6.21 mg/g.
26698559	3	14	theme	6.21	664:667	arg1	capacity					630:637	The obtained monolayer adsorption capacity	596:637	The obtained monolayer adsorption capacity of a-ASC for Cd (II)	596:658	The obtained monolayer adsorption capacity of a-ASC for Cd (II) was 6.21 mg/g.
26698559	6	15	theme	costly	1097:1102	arg1	adsorbents					1104:1113	more costly adsorbents	1092:1113	more costly adsorbents used for the removal of heavy metals	1092:1150	The results indicated that a-ASC could be an alternative material replacing more costly adsorbents used for the removal of heavy metals.
26698559	1	16	theme	Cd	158:159	arg1	ions					166:169	Cd (II) ions	158:169	Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate	158:289	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	0	17	theme	Triplochiton	77:88	arg1	sawdust					68:74	alginate-Ayous wood sawdust	48:74	alginate-Ayous wood sawdust (Triplochiton scleroxylon)	48:101	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon) composite material.
26698559	0	17	theme	Triplochiton	77:88	arg1	scleroxylon					90:100	Triplochiton scleroxylon	77:100	Triplochiton scleroxylon	77:100	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon) composite material.
26698559	5	18	theme	Cd	909:910	arg1	feasible					941:948	feasible	941:948	feasible	941:948	Thermodynamic calculations showed that the Cd (II) biosorption process was feasible, endothermic and spontaneous in nature under examined conditions.
26698559	5	18	theme	Cd	909:910	arg1	process					929:935	the Cd (II) biosorption process	905:935	the Cd (II) biosorption process	905:935	Thermodynamic calculations showed that the Cd (II) biosorption process was feasible, endothermic and spontaneous in nature under examined conditions.
26698559	1	19	theme	biomass	329:335	arg1	quantity					337:344	biomass quantity	329:344	biomass quantity	329:344	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	20	theme	stirring	415:422	arg1	rate					424:427	stirring rate	415:427	stirring rate	415:427	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	21	theme	II	162:163	arg1	ions					166:169	Cd (II) ions	158:169	Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate	158:289	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	5	22	from	feasible	941:948	arg1	nature					982:987	nature	982:987	nature	982:987	Thermodynamic calculations showed that the Cd (II) biosorption process was feasible, endothermic and spontaneous in nature under examined conditions.
26698559	0	23	theme	composite	103:111	arg1	material					113:120	composite material	103:120	composite material	103:120	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon) composite material.
26698559	6	24	theme	heavy	1139:1143	arg1	metals					1145:1150	heavy metals	1139:1150	heavy metals	1139:1150	The results indicated that a-ASC could be an alternative material replacing more costly adsorbents used for the removal of heavy metals.
26698559	5	25	theme	Thermodynamic	866:878	arg1	calculations					880:891	Thermodynamic calculations	866:891	Thermodynamic calculations	866:891	Thermodynamic calculations showed that the Cd (II) biosorption process was feasible, endothermic and spontaneous in nature under examined conditions.
26698559	1	26	theme	ions	166:169	arg1	characteristics					139:153	The biosorption characteristics	123:153	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate	123:289	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	0	27	theme	Cd	11:12	arg1	Removal					0:6	Removal	0:6	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon)	0:101	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon) composite material.
26698559	4	28	theme	physisorption	845:857	arg1	stage					859:863	an important physisorption stage	832:863	an important physisorption stage	832:863	From the Dubinin-Radushkevich isotherm model, a 5.39 kJ/mol value for the mean free energy was calculated, indicating that Cd (II) biosorption could include an important physisorption stage.
26698559	3	29	theme	obtained	600:607	arg1	mg/g					669:672	6.21 mg/g	664:672	6.21 mg/g	664:672	The obtained monolayer adsorption capacity of a-ASC for Cd (II) was 6.21 mg/g.
26698559	3	29	theme	obtained	600:607	arg1	capacity					630:637	The obtained monolayer adsorption capacity	596:637	The obtained monolayer adsorption capacity of a-ASC for Cd (II)	596:658	The obtained monolayer adsorption capacity of a-ASC for Cd (II) was 6.21 mg/g.
26698559	1	30	theme	synthetic	176:184	arg1	wastewater					186:195	synthetic wastewater	176:195	synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate	176:289	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	4	31	theme	important	835:843	arg1	stage					859:863	an important physisorption stage	832:863	an important physisorption stage	832:863	From the Dubinin-Radushkevich isotherm model, a 5.39 kJ/mol value for the mean free energy was calculated, indicating that Cd (II) biosorption could include an important physisorption stage.
26698559	1	32	theme	contact	347:353	arg1	time					355:358	contact time	347:358	contact time	347:358	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	0	33	theme	synthetic	24:32	arg1	wastewater					34:43	synthetic wastewater	24:43	synthetic wastewater	24:43	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon) composite material.
26698559	0	34	from	wastewater	34:43	arg1	Removal					0:6	Removal	0:6	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon)	0:101	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon) composite material.
26698559	4	35	theme	free	754:757	arg1	energy					759:764	the mean free energy	745:764	the mean free energy	745:764	From the Dubinin-Radushkevich isotherm model, a 5.39 kJ/mol value for the mean free energy was calculated, indicating that Cd (II) biosorption could include an important physisorption stage.
26698559	1	36	from	wastewater	186:195	arg1	characteristics					139:153	The biosorption characteristics	123:153	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate	123:289	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	36	from	wastewater	186:195	arg1	ions					166:169	Cd (II) ions	158:169	Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate	158:289	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	37	theme	sodium	275:280	arg1	alginate					282:289	sodium alginate	275:289	sodium alginate	275:289	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	4	38	theme	mean	749:752	arg1	energy					759:764	the mean free energy	745:764	the mean free energy	745:764	From the Dubinin-Radushkevich isotherm model, a 5.39 kJ/mol value for the mean free energy was calculated, indicating that Cd (II) biosorption could include an important physisorption stage.
26698559	5	39	theme	examined	995:1002	arg1	conditions					1004:1013	examined conditions	995:1013	examined conditions	995:1013	Thermodynamic calculations showed that the Cd (II) biosorption process was feasible, endothermic and spontaneous in nature under examined conditions.
26698559	5	40	theme	biosorption	917:927	arg1	feasible					941:948	feasible	941:948	feasible	941:948	Thermodynamic calculations showed that the Cd (II) biosorption process was feasible, endothermic and spontaneous in nature under examined conditions.
26698559	5	40	theme	biosorption	917:927	arg1	process					929:935	the Cd (II) biosorption process	905:935	the Cd (II) biosorption process	905:935	Thermodynamic calculations showed that the Cd (II) biosorption process was feasible, endothermic and spontaneous in nature under examined conditions.
26698559	1	41	theme	initial	361:367	arg1	concentration					369:381	initial concentration	361:381	initial concentration of heavy metal	361:396	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	4	42	theme	5.39	723:726	arg1	kJ/mol					728:733	kJ/mol	728:733	kJ/mol	728:733	From the Dubinin-Radushkevich isotherm model, a 5.39 kJ/mol value for the mean free energy was calculated, indicating that Cd (II) biosorption could include an important physisorption stage.
26698559	6	43	theme	alternative	1061:1071	arg1	material					1073:1080	an alternative material	1058:1080	an alternative material replacing more costly adsorbents used for the removal of heavy metals	1058:1150	The results indicated that a-ASC could be an alternative material replacing more costly adsorbents used for the removal of heavy metals.
26698559	6	43	theme	alternative	1061:1071	arg1	a-ASC					1043:1047	a-ASC	1043:1047	a-ASC	1043:1047	The results indicated that a-ASC could be an alternative material replacing more costly adsorbents used for the removal of heavy metals.
26698559	2	44	theme	monolayer	508:516	arg1	adsorption					518:527	monolayer adsorption	508:527	monolayer adsorption of the cadmium ions onto alginate-Ayous	508:567	The experimental data fitted well with the Langmuir isotherm, suggesting that monolayer adsorption of the cadmium ions onto alginate-Ayous sawdust composite (a-ASC).
26698559	3	45	theme	monolayer	609:617	arg1	mg/g					669:672	6.21 mg/g	664:672	6.21 mg/g	664:672	The obtained monolayer adsorption capacity of a-ASC for Cd (II) was 6.21 mg/g.
26698559	3	45	theme	monolayer	609:617	arg1	capacity					630:637	The obtained monolayer adsorption capacity	596:637	The obtained monolayer adsorption capacity of a-ASC for Cd (II)	596:658	The obtained monolayer adsorption capacity of a-ASC for Cd (II) was 6.21 mg/g.
26698559	0	46	dep	material	113:120	arg1	Removal					0:6	Removal	0:6	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon)	0:101	Removal of Cd (II) from synthetic wastewater by alginate-Ayous wood sawdust (Triplochiton scleroxylon) composite material.
26698559	1	47	theme	raw	203:205	arg1	r-AS					254:257	r-AS	254:257	r-AS	254:257	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	47	theme	raw	203:205	arg1	sawdust					218:224	raw Ayous wood sawdust	203:224	raw Ayous wood sawdust (Triplochiton scleroxylon)	203:251	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	47	theme	raw	203:205	arg1	scleroxylon					240:250	Triplochiton scleroxylon	227:250	Triplochiton scleroxylon	227:250	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	3	48	theme	adsorption	619:628	arg1	mg/g					669:672	6.21 mg/g	664:672	6.21 mg/g	664:672	The obtained monolayer adsorption capacity of a-ASC for Cd (II) was 6.21 mg/g.
26698559	3	48	theme	adsorption	619:628	arg1	capacity					630:637	The obtained monolayer adsorption capacity	596:637	The obtained monolayer adsorption capacity of a-ASC for Cd (II)	596:658	The obtained monolayer adsorption capacity of a-ASC for Cd (II) was 6.21 mg/g.
26698559	6	49	theme	metals	1145:1150	arg1	removal					1128:1134	the removal	1124:1134	the removal of heavy metals	1124:1150	The results indicated that a-ASC could be an alternative material replacing more costly adsorbents used for the removal of heavy metals.
26698559	1	50	theme	Ayous	207:211	arg1	r-AS					254:257	r-AS	254:257	r-AS	254:257	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	50	theme	Ayous	207:211	arg1	sawdust					218:224	raw Ayous wood sawdust	203:224	raw Ayous wood sawdust (Triplochiton scleroxylon)	203:251	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	50	theme	Ayous	207:211	arg1	scleroxylon					240:250	Triplochiton scleroxylon	227:250	Triplochiton scleroxylon	227:250	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	4	51	theme	kJ/mol	728:733	arg1	value					735:739	a 5.39 kJ/mol value	721:739	a 5.39 kJ/mol value for the mean free energy	721:764	From the Dubinin-Radushkevich isotherm model, a 5.39 kJ/mol value for the mean free energy was calculated, indicating that Cd (II) biosorption could include an important physisorption stage.
26698559	1	52	theme	biosorption	127:137	arg1	characteristics					139:153	The biosorption characteristics	123:153	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate	123:289	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	53	theme	wood	213:216	arg1	r-AS					254:257	r-AS	254:257	r-AS	254:257	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	53	theme	wood	213:216	arg1	sawdust					218:224	raw Ayous wood sawdust	203:224	raw Ayous wood sawdust (Triplochiton scleroxylon)	203:251	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
26698559	1	53	theme	wood	213:216	arg1	scleroxylon					240:250	Triplochiton scleroxylon	227:250	Triplochiton scleroxylon	227:250	The biosorption characteristics of Cd (II) ions from synthetic wastewater using raw Ayous wood sawdust (Triplochiton scleroxylon), r-AS, immobilized by sodium alginate were investigated with respect to pH, biomass quantity, contact time, initial concentration of heavy metal, temperature and stirring rate.
28636921	10	0	theme	new	1457:1459	arg1	protocols					1492:1500	new and improved cell preservation protocols	1457:1500	new and improved cell preservation protocols	1457:1500	Raman spectromicroscopy provides a powerful tool for observing IIF and understanding its role in cell death during freezing, and enables the development, to our knowledge, of new and improved cell preservation protocols.
28636921	7	1	theme	cell	983:986	arg1	membrane					988:995	the cell membrane	979:995	the cell membrane	979:995	Reducing the effective stiffness of the cell membrane by disrupting the actin cytoskeleton using cytochalasin D increased the amount of IIF.
28636921	5	2	theme	ice	741:743	arg1	crystals					745:752	Intracellular ice crystals	727:752	Intracellular ice crystals	727:752	Intracellular ice crystals were colocated to the sections of cell membrane in close proximity to extracellular ice.
28636921	4	3	theme	intracellular	597:609	arg1	ice					611:613	intracellular ice	597:613	intracellular ice	597:613	The amount of intracellular ice as well as ice crystal size played a role in determining whether or not ice inside the cell was a lethal event.
28636921	2	4	from	pattern	242:248	arg1	cells					280:284	individual cells	269:284	individual cells	269:284	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	2	5	theme	viability	316:324	arg1	pattern					242:248	The distribution pattern	225:248	The distribution pattern of cytochrome c in individual cells	225:284	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	2	5	theme	viability	316:324	arg1	measure					300:306	a measure	298:306	a measure of cell viability	298:324	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	10	6	theme	cell	1379:1382	arg1	death					1384:1388	cell death	1379:1388	cell death	1379:1388	Raman spectromicroscopy provides a powerful tool for observing IIF and understanding its role in cell death during freezing, and enables the development, to our knowledge, of new and improved cell preservation protocols.
28636921	3	7	theme	cell	571:574	arg1	death					576:580	cell death	571:580	cell death	571:580	Raman imaging of cells demonstrated that intracellular ice formation (IIF) was common and did not necessarily result in cell death.
28636921	6	8	theme	cell	897:900	arg1	membrane					902:909	cell membrane	897:909	cell membrane	897:909	Increasing the distance between extracellular ice and cell membrane decreased the incidence of IIF.
28636921	1	9	theme	Raman	101:105	arg1	microspectroscopy					107:123	Raman microspectroscopy	101:123	Raman microspectroscopy	101:123	Raman microspectroscopy was used to quantify freezing response of cells to various cooling rates and solution compositions.
28636921	2	10	theme	cell	311:314	arg1	viability					316:324	cell viability	311:324	cell viability	311:324	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	4	11	theme	ice	611:613	arg1	ice					611:613	intracellular ice	597:613	intracellular ice	597:613	The amount of intracellular ice as well as ice crystal size played a role in determining whether or not ice inside the cell was a lethal event.
28636921	4	11	theme	ice	611:613	arg1	size					638:641	ice crystal size	626:641	The amount of intracellular ice as well as ice crystal size	583:641	The amount of intracellular ice as well as ice crystal size played a role in determining whether or not ice inside the cell was a lethal event.
28636921	4	11	theme	ice	611:613	arg1	amount					587:592	The amount	583:592	The amount of intracellular ice as well as ice crystal size	583:641	The amount of intracellular ice as well as ice crystal size played a role in determining whether or not ice inside the cell was a lethal event.
28636921	5	12	theme	Intracellular	727:739	arg1	crystals					745:752	Intracellular ice crystals	727:752	Intracellular ice crystals	727:752	Intracellular ice crystals were colocated to the sections of cell membrane in close proximity to extracellular ice.
28636921	5	13	theme	extracellular	824:836	arg1	ice					838:840	extracellular ice	824:840	extracellular ice	824:840	Intracellular ice crystals were colocated to the sections of cell membrane in close proximity to extracellular ice.
28636921	5	14	theme	membrane	793:800	arg1	sections					776:783	the sections	772:783	the sections of cell membrane	772:800	Intracellular ice crystals were colocated to the sections of cell membrane in close proximity to extracellular ice.
28636921	2	15	used	used	290:293	arg2	measure					300:306	a measure	298:306	a measure of cell viability	298:324	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	2	15	used	used	290:293	arg2	pattern					242:248	The distribution pattern	225:248	The distribution pattern of cytochrome c in individual cells	225:284	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	1	16	theme	solution	202:209	arg1	compositions					211:222	solution compositions	202:222	solution compositions	202:222	Raman microspectroscopy was used to quantify freezing response of cells to various cooling rates and solution compositions.
28636921	5	17	theme	cell	788:791	arg1	membrane					793:800	cell membrane	788:800	cell membrane	788:800	Intracellular ice crystals were colocated to the sections of cell membrane in close proximity to extracellular ice.
28636921	10	18	theme	cell	1474:1477	arg1	protocols					1492:1500	new and improved cell preservation protocols	1457:1500	new and improved cell preservation protocols	1457:1500	Raman spectromicroscopy provides a powerful tool for observing IIF and understanding its role in cell death during freezing, and enables the development, to our knowledge, of new and improved cell preservation protocols.
28636921	10	19	theme	protocols	1492:1500	arg1	development					1423:1433	the development	1419:1433	the development	1419:1433	Raman spectromicroscopy provides a powerful tool for observing IIF and understanding its role in cell death during freezing, and enables the development, to our knowledge, of new and improved cell preservation protocols.
28636921	2	20	theme	trypan	417:422	arg1	staining					429:436	trypan blue staining	417:436	trypan blue staining	417:436	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	0	21	theme	Intracellular	15:27	arg1	Formation					33:41	Intracellular Ice Formation	15:41	Intracellular Ice Formation	15:41	Characterizing Intracellular Ice Formation of Lymphoblasts Using Low-Temperature Raman Spectroscopy.
28636921	6	22	theme	extracellular	875:887	arg1	ice					889:891	extracellular ice	875:891	extracellular ice	875:891	Increasing the distance between extracellular ice and cell membrane decreased the incidence of IIF.
28636921	10	23	theme	improved	1465:1472	arg1	protocols					1492:1500	new and improved cell preservation protocols	1457:1500	new and improved cell preservation protocols	1457:1500	Raman spectromicroscopy provides a powerful tool for observing IIF and understanding its role in cell death during freezing, and enables the development, to our knowledge, of new and improved cell preservation protocols.
28636921	9	24	theme	cell	1231:1234	arg1	membrane					1236:1243	the cell membrane	1227:1243	the cell membrane	1227:1243	These observations support the hypothesis that interactions between the cell membrane and extracellular ice result in IIF.
28636921	2	25	theme	frozen	333:338	arg1	state					340:344	the frozen state	329:344	the frozen state	329:344	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	2	26	theme	viability	403:411	arg1	experiments					438:448	the population-averaged viability and trypan blue staining experiments	379:448	the population-averaged viability and trypan blue staining experiments	379:448	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	5	27	theme	close	805:809	arg1	proximity					811:819	close proximity	805:819	close proximity to extracellular ice	805:840	Intracellular ice crystals were colocated to the sections of cell membrane in close proximity to extracellular ice.
28636921	8	28	theme	Strong	1084:1089	arg1	gradients					1113:1121	Strong intracellular osmotic gradients	1084:1121	Strong intracellular osmotic gradients	1084:1121	Strong intracellular osmotic gradients were observed when IIF was present.
28636921	7	29	theme	effective	956:964	arg1	stiffness					966:974	the effective stiffness	952:974	the effective stiffness of the cell membrane	952:995	Reducing the effective stiffness of the cell membrane by disrupting the actin cytoskeleton using cytochalasin D increased the amount of IIF.
28636921	3	30	theme	ice	506:508	arg1	IIF					521:523	IIF	521:523	IIF	521:523	Raman imaging of cells demonstrated that intracellular ice formation (IIF) was common and did not necessarily result in cell death.
28636921	3	30	theme	ice	506:508	arg1	formation					510:518	intracellular ice formation	492:518	intracellular ice formation (IIF)	492:524	Raman imaging of cells demonstrated that intracellular ice formation (IIF) was common and did not necessarily result in cell death.
28636921	0	31	theme	Ice	29:31	arg1	Formation					33:41	Intracellular Ice Formation	15:41	Intracellular Ice Formation	15:41	Characterizing Intracellular Ice Formation of Lymphoblasts Using Low-Temperature Raman Spectroscopy.
28636921	7	32	theme	IIF	1079:1081	arg1	amount					1069:1074	the amount	1065:1074	the amount of IIF	1065:1081	Reducing the effective stiffness of the cell membrane by disrupting the actin cytoskeleton using cytochalasin D increased the amount of IIF.
28636921	7	32	theme	IIF	1079:1081	arg1	IIF					1079:1081	IIF	1079:1081	IIF	1079:1081	Reducing the effective stiffness of the cell membrane by disrupting the actin cytoskeleton using cytochalasin D increased the amount of IIF.
28636921	9	33	theme	extracellular	1249:1261	arg1	ice					1263:1265	extracellular ice	1249:1265	extracellular ice	1249:1265	These observations support the hypothesis that interactions between the cell membrane and extracellular ice result in IIF.
28636921	1	34	theme	freezing	146:153	arg1	response					155:162	freezing response	146:162	freezing response of cells to various cooling rates and solution compositions	146:222	Raman microspectroscopy was used to quantify freezing response of cells to various cooling rates and solution compositions.
28636921	1	35	theme	various	176:182	arg1	rates					192:196	various cooling rates	176:196	various cooling rates	176:196	Raman microspectroscopy was used to quantify freezing response of cells to various cooling rates and solution compositions.
28636921	10	36	theme	powerful	1317:1324	arg1	tool					1326:1329	a powerful tool	1315:1329	a powerful tool for observing IIF and understanding its role in cell death during freezing	1315:1404	Raman spectromicroscopy provides a powerful tool for observing IIF and understanding its role in cell death during freezing, and enables the development, to our knowledge, of new and improved cell preservation protocols.
28636921	8	37	theme	osmotic	1105:1111	arg1	gradients					1113:1121	Strong intracellular osmotic gradients	1084:1121	Strong intracellular osmotic gradients	1084:1121	Strong intracellular osmotic gradients were observed when IIF was present.
28636921	10	38	from	role	1371:1374	arg1	death					1384:1388	cell death	1379:1388	cell death	1379:1388	Raman spectromicroscopy provides a powerful tool for observing IIF and understanding its role in cell death during freezing, and enables the development, to our knowledge, of new and improved cell preservation protocols.
28636921	2	39	theme	blue	424:427	arg1	staining					429:436	trypan blue staining	417:436	trypan blue staining	417:436	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	3	40	theme	Raman	451:455	arg1	imaging					457:463	Raman imaging	451:463	Raman imaging of cells	451:472	Raman imaging of cells demonstrated that intracellular ice formation (IIF) was common and did not necessarily result in cell death.
28636921	10	41	theme	Raman	1282:1286	arg1	spectromicroscopy					1288:1304	Raman spectromicroscopy	1282:1304	Raman spectromicroscopy	1282:1304	Raman spectromicroscopy provides a powerful tool for observing IIF and understanding its role in cell death during freezing, and enables the development, to our knowledge, of new and improved cell preservation protocols.
28636921	2	42	theme	individual	269:278	arg1	cells					280:284	individual cells	269:284	individual cells	269:284	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	7	43	theme	actin	1015:1019	arg1	cytoskeleton					1021:1032	the actin cytoskeleton	1011:1032	the actin cytoskeleton using cytochalasin D	1011:1053	Reducing the effective stiffness of the cell membrane by disrupting the actin cytoskeleton using cytochalasin D increased the amount of IIF.
28636921	2	44	theme	staining	429:436	arg1	experiments					438:448	the population-averaged viability and trypan blue staining experiments	379:448	the population-averaged viability and trypan blue staining experiments	379:448	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	3	45	theme	cells	468:472	arg1	imaging					457:463	Raman imaging	451:463	Raman imaging of cells	451:472	Raman imaging of cells demonstrated that intracellular ice formation (IIF) was common and did not necessarily result in cell death.
28636921	3	46	theme	intracellular	492:504	arg1	IIF					521:523	IIF	521:523	IIF	521:523	Raman imaging of cells demonstrated that intracellular ice formation (IIF) was common and did not necessarily result in cell death.
28636921	3	46	theme	intracellular	492:504	arg1	formation					510:518	intracellular ice formation	492:518	intracellular ice formation (IIF)	492:524	Raman imaging of cells demonstrated that intracellular ice formation (IIF) was common and did not necessarily result in cell death.
28636921	0	47	theme	Low-Temperature	65:79	arg1	Raman Spectroscopy					81:98	Low-Temperature Raman Spectroscopy	65:98	Low-Temperature Raman Spectroscopy	65:98	Characterizing Intracellular Ice Formation of Lymphoblasts Using Low-Temperature Raman Spectroscopy.
28636921	6	48	theme	IIF	938:940	arg1	incidence					925:933	the incidence	921:933	the incidence of IIF	921:940	Increasing the distance between extracellular ice and cell membrane decreased the incidence of IIF.
28636921	2	49	theme	cytochrome	253:262	arg1	c					264:264	cytochrome c	253:264	cytochrome c	253:264	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	2	50	theme	c	264:264	arg1	pattern					242:248	The distribution pattern	225:248	The distribution pattern of cytochrome c in individual cells	225:284	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	2	50	theme	c	264:264	arg1	measure					300:306	a measure	298:306	a measure of cell viability	298:324	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	10	51	theme	preservation	1479:1490	arg1	protocols					1492:1500	new and improved cell preservation protocols	1457:1500	new and improved cell preservation protocols	1457:1500	Raman spectromicroscopy provides a powerful tool for observing IIF and understanding its role in cell death during freezing, and enables the development, to our knowledge, of new and improved cell preservation protocols.
28636921	1	52	theme	cells	167:171	arg1	response					155:162	freezing response	146:162	freezing response of cells to various cooling rates and solution compositions	146:222	Raman microspectroscopy was used to quantify freezing response of cells to various cooling rates and solution compositions.
28636921	7	53	theme	cytochalasin	1040:1051	arg1	D					1053:1053	cytochalasin D	1040:1053	cytochalasin D	1040:1053	Reducing the effective stiffness of the cell membrane by disrupting the actin cytoskeleton using cytochalasin D increased the amount of IIF.
28636921	7	54	theme	membrane	988:995	arg1	stiffness					966:974	the effective stiffness	952:974	the effective stiffness of the cell membrane	952:995	Reducing the effective stiffness of the cell membrane by disrupting the actin cytoskeleton using cytochalasin D increased the amount of IIF.
28636921	1	55	used	used	129:132	arg2	microspectroscopy					107:123	Raman microspectroscopy	101:123	Raman microspectroscopy	101:123	Raman microspectroscopy was used to quantify freezing response of cells to various cooling rates and solution compositions.
28636921	4	56	theme	lethal	713:718	arg1	event					720:724	a lethal event	711:724	a lethal event	711:724	The amount of intracellular ice as well as ice crystal size played a role in determining whether or not ice inside the cell was a lethal event.
28636921	2	57	theme	distribution	229:240	arg1	pattern					242:248	The distribution pattern	225:248	The distribution pattern of cytochrome c in individual cells	225:284	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	2	57	theme	distribution	229:240	arg1	measure					300:306	a measure	298:306	a measure of cell viability	298:324	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	2	58	theme	population-averaged	383:401	arg1	viability					403:411	population-averaged viability	383:411	population-averaged viability	383:411	The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments.
28636921	4	59	theme	crystal	630:636	arg1	size					638:641	ice crystal size	626:641	The amount of intracellular ice as well as ice crystal size	583:641	The amount of intracellular ice as well as ice crystal size played a role in determining whether or not ice inside the cell was a lethal event.
28636921	1	60	theme	cooling	184:190	arg1	rates					192:196	various cooling rates	176:196	various cooling rates	176:196	Raman microspectroscopy was used to quantify freezing response of cells to various cooling rates and solution compositions.
28636921	8	61	theme	intracellular	1091:1103	arg1	gradients					1113:1121	Strong intracellular osmotic gradients	1084:1121	Strong intracellular osmotic gradients	1084:1121	Strong intracellular osmotic gradients were observed when IIF was present.
28636921	4	62	theme	ice	626:628	arg1	size					638:641	ice crystal size	626:641	The amount of intracellular ice as well as ice crystal size	583:641	The amount of intracellular ice as well as ice crystal size played a role in determining whether or not ice inside the cell was a lethal event.
27738635	0	0	theme	Biomethane	80:89	arg1	Production					91:100	Biomethane Production	80:100	Biomethane Production	80:100	Alkaline Pretreatment of Sugarcane Bagasse and Filter Mud Codigested to Improve Biomethane Production.
27738635	2	1	theme	%	443:443	arg1	temperature					394:404	mesophilic temperature	383:404	mesophilic temperature	383:404	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	2	1	theme	%	443:443	arg1	±					410:410	37 ± 1	407:412	37 ± 1	407:412	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	2	1	theme	%	443:443	arg1	°C					414:415	°C	414:415	°C	414:415	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	2	1	theme	%	443:443	arg1	concentrations					424:437	solid concentrations	418:437	solid concentrations of 6%	418:443	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	2	1	theme	%	443:443	arg1	levels					455:460	five levels	450:460	five levels of mixing proportion with and without pretreatment	450:511	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	4	2	theme	significant	1206:1216	arg1	increase					1218:1225	significant increase	1206:1225	significant increase in cumulative methane yield	1206:1253	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	1	3	theme	biomethane	157:166	arg1	potential					179:187	biomethane production potential	157:187	biomethane production potential	157:187	To enhance the codigestion of degradation and improve biomethane production potential, sugarcane bagasse and filter mud were pretreated by sodium hydroxide NaOH 1 N at 100°C for 15, 30, and 45 minutes, respectively.
27738635	4	4	theme	matter	957:962	arg1	solubilization					927:940	solubilization	927:940	solubilization of the organic matter	927:962	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	1	5	theme	NaOH	259:262	arg1	1 N					264:266	sodium hydroxide NaOH 1 N	242:266	sodium hydroxide NaOH 1 N	242:266	To enhance the codigestion of degradation and improve biomethane production potential, sugarcane bagasse and filter mud were pretreated by sodium hydroxide NaOH 1 N at 100°C for 15, 30, and 45 minutes, respectively.
27738635	4	6	dep	observed	1259:1266	arg1	126.2 mL·gVS-1					1269:1282	126.2 mL·gVS-1	1269:1282	126.2 mL·gVS-1	1269:1282	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	7	dep	led	920:922	arg1	 75					1313:1315	 75	1313:1315	 75 between filter mud and bagasse by increase of 81.20% from untreated composition	1313:1395	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	1	8	theme	production	168:177	arg1	potential					179:187	biomethane production potential	157:187	biomethane production potential	157:187	To enhance the codigestion of degradation and improve biomethane production potential, sugarcane bagasse and filter mud were pretreated by sodium hydroxide NaOH 1 N at 100°C for 15, 30, and 45 minutes, respectively.
27738635	2	9	theme	mixing	465:470	arg1	proportion					472:481	mixing proportion	465:481	mixing proportion with and without pretreatment	465:511	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	3	10	theme	single	642:647	arg1	substrate					649:657	single substrate	642:657	single substrate	642:657	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	4	11	from	composition	1385:1395	arg1	increase					1351:1358	increase	1351:1358	increase of 81.20% from untreated composition	1351:1395	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	11	from	composition	1385:1395	arg1	%					1368:1368	81.20%	1363:1368	81.20% from untreated composition	1363:1395	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	3	12	theme	substrate	676:684	arg1	composition					686:696	even codigested substrate composition	660:696	even codigested substrate composition	660:696	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	4	13	theme	pretreated	1072:1081	arg1	bagasse					1083:1089	pretreated bagasse	1072:1089	pretreated bagasse as single substrate	1072:1109	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	14	theme	cooking	1141:1147	arg1	minutes					1130:1136	45 minutes	1127:1136	45 minutes of cooking	1127:1147	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	3	15	theme	codigestion	764:774	arg1	ratio					776:780	codigestion ratio	764:780	codigestion ratio between filter mud and bagasse	764:811	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	3	16	with	codigestion	543:553	arg1	bagasse					574:580	bagasse	574:580	bagasse	574:580	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	3	17	dep	nutrients	728:736	arg1	ratio					743:747	C/N ratio	739:747	C/N ratio of 24.70	739:756	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	4	18	from	increase	1218:1225	arg1	yield					1249:1253	cumulative methane yield	1230:1253	cumulative methane yield	1230:1253	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	3	19	theme	filter	842:847	arg1	mud					849:851	filter mud	842:851	filter mud	842:851	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	4	20	theme	%	1368:1368	arg1	increase					1351:1358	increase	1351:1358	increase of 81.20% from untreated composition	1351:1395	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	21	theme	%	1005:1005	arg1	yield					1022:1026	86.27% and cumulative yield	1000:1026	86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 	1000:1311	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	22	dep	100°C.	1164:1169	arg1	observed					1259:1266	observed	1259:1266	was observed (126.2 mL·gVS-1) at codigestion ratio of 25 	1255:1311	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	23	theme	filter	1325:1330	arg1	mud					1332:1334	filter mud	1325:1334	filter mud	1325:1334	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	24	theme	untreated	1375:1383	arg1	composition					1385:1395	untreated composition	1375:1395	untreated composition	1375:1395	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	3	25	theme	codigested	665:674	arg1	composition					686:696	even codigested substrate composition	660:696	even codigested substrate composition	660:696	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	3	26	dep	25 	817:819	arg1	 75					821:823	 75	821:823	 75	821:823	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	4	27	theme	biomethane	1031:1040	arg1	yield					1022:1026	86.27% and cumulative yield	1000:1026	86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 	1000:1311	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	1	28	theme	sugarcane	190:198	arg1	bagasse					200:206	sugarcane bagasse	190:206	sugarcane bagasse	190:206	To enhance the codigestion of degradation and improve biomethane production potential, sugarcane bagasse and filter mud were pretreated by sodium hydroxide NaOH 1 N at 100°C for 15, 30, and 45 minutes, respectively.
27738635	0	29	theme	Alkaline	0:7	arg1	Pretreatment					9:20	Alkaline Pretreatment	0:20	Alkaline Pretreatment of Sugarcane Bagasse and Filter Mud	0:56	Alkaline Pretreatment of Sugarcane Bagasse and Filter Mud Codigested to Improve Biomethane Production.
27738635	3	30	theme	better	710:715	arg1	balance					717:723	a better balance	708:723	a better balance of nutrients (C/N ratio of 24.70)	708:757	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	4	31	located	observed	1259:1266	arg2	increase					1218:1225	significant increase	1206:1225	significant increase in cumulative methane yield	1206:1253	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	31	located	observed	1259:1266	arg1	ratio					1300:1304	codigestion ratio	1288:1304	codigestion ratio of 25 	1288:1311	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	3	32	theme	single	856:861	arg1	ratio					878:882	C/N ratio 9.68	874:887	C/N ratio 9.68	874:887	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	3	32	theme	single	856:861	arg1	substrate					863:871	single substrate	856:871	single substrate (C/N ratio 9.68)	856:888	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	0	33	theme	Sugarcane	25:33	arg1	Bagasse					35:41	Sugarcane Bagasse	25:41	Sugarcane Bagasse	25:41	Alkaline Pretreatment of Sugarcane Bagasse and Filter Mud Codigested to Improve Biomethane Production.
27738635	4	34	theme	86.27	1000:1004	arg1	%					1005:1005	%	1005:1005	%	1005:1005	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	35	dep	195.8 mL·gVS-1	1043:1056	arg1	digestion					1059:1067	digestion	1059:1067	digestion of pretreated bagasse as single substrate	1059:1109	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	36	theme	25 	1309:1311	arg1	ratio					1300:1304	codigestion ratio	1288:1304	codigestion ratio of 25 	1288:1311	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	37	theme	cumulative	1011:1020	arg1	yield					1022:1026	86.27% and cumulative yield	1000:1026	86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 	1000:1311	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	1	38	theme	filter	212:217	arg1	mud					219:221	filter mud	212:221	filter mud	212:221	To enhance the codigestion of degradation and improve biomethane production potential, sugarcane bagasse and filter mud were pretreated by sodium hydroxide NaOH 1 N at 100°C for 15, 30, and 45 minutes, respectively.
27738635	4	39	theme	codigestion	1288:1298	arg1	ratio					1300:1304	codigestion ratio	1288:1304	codigestion ratio of 25 	1288:1311	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	0	40	theme	Bagasse	35:41	arg1	Pretreatment					9:20	Alkaline Pretreatment	0:20	Alkaline Pretreatment of Sugarcane Bagasse and Filter Mud	0:56	Alkaline Pretreatment of Sugarcane Bagasse and Filter Mud Codigested to Improve Biomethane Production.
27738635	2	41	theme	proportion	472:481	arg1	temperature					394:404	mesophilic temperature	383:404	mesophilic temperature	383:404	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	2	41	theme	proportion	472:481	arg1	±					410:410	37 ± 1	407:412	37 ± 1	407:412	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	2	41	theme	proportion	472:481	arg1	°C					414:415	°C	414:415	°C	414:415	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	2	41	theme	proportion	472:481	arg1	concentrations					424:437	solid concentrations	418:437	solid concentrations of 6%	418:443	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	2	41	theme	proportion	472:481	arg1	levels					455:460	five levels	450:460	five levels of mixing proportion with and without pretreatment	450:511	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	4	42	theme	methane	1241:1247	arg1	yield					1249:1253	cumulative methane yield	1230:1253	cumulative methane yield	1230:1253	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	43	theme	organic	949:955	arg1	matter					957:962	the organic matter	945:962	the organic matter	945:962	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	44	theme	bagasse	1083:1089	arg1	digestion					1059:1067	digestion	1059:1067	digestion of pretreated bagasse as single substrate	1059:1109	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	2	45	theme	mesophilic	383:392	arg1	temperature					394:404	mesophilic temperature	383:404	mesophilic temperature	383:404	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	2	45	theme	mesophilic	383:392	arg1	±					410:410	37 ± 1	407:412	37 ± 1	407:412	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	0	46	theme	Mud	54:56	arg1	Pretreatment					9:20	Alkaline Pretreatment	0:20	Alkaline Pretreatment of Sugarcane Bagasse and Filter Mud	0:56	Alkaline Pretreatment of Sugarcane Bagasse and Filter Mud Codigested to Improve Biomethane Production.
27738635	3	47	theme	C/N	739:741	arg1	ratio					743:747	C/N ratio	739:747	C/N ratio of 24.70	739:756	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	4	48	theme	yield	1022:1026	arg1	reduction					987:995	a maximum lignin reduction	970:995	a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 	970:1311	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	2	49	from	reactor	360:366	arg1	generation					330:339	Biomethane generation	319:339	Biomethane generation from 1-liter batch reactor	319:366	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	2	50	theme	Biomethane	319:328	arg1	generation					330:339	Biomethane generation	319:339	Biomethane generation from 1-liter batch reactor	319:366	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	0	51	theme	Filter	47:52	arg1	Mud					54:56	Filter Mud	47:56	Filter Mud	47:56	Alkaline Pretreatment of Sugarcane Bagasse and Filter Mud Codigested to Improve Biomethane Production.
27738635	3	52	theme	filter	628:633	arg1	mud					635:637	filter mud	628:637	filter mud	628:637	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	4	53	theme	single	1094:1099	arg1	substrate					1101:1109	single substrate	1094:1109	single substrate	1094:1109	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	54	theme	pretreatment	1182:1193	arg1	condition					1195:1203	this pretreatment condition	1177:1203	this pretreatment condition	1177:1203	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	4	55	theme	lignin	980:985	arg1	reduction					987:995	a maximum lignin reduction	970:995	a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 	970:1311	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	3	56	theme	nutrients	728:736	arg1	balance					717:723	a better balance	708:723	a better balance of nutrients (C/N ratio of 24.70)	708:757	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	3	57	theme	mud	635:637	arg1	fermentation					612:623	fermentation	612:623	fermentation of filter mud	612:637	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	3	58	theme	C/N	874:876	arg1	ratio					878:882	C/N ratio 9.68	874:887	C/N ratio 9.68	874:887	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	3	58	theme	C/N	874:876	arg1	substrate					863:871	single substrate	856:871	single substrate (C/N ratio 9.68)	856:888	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	4	59	theme	maximum	972:978	arg1	reduction					987:995	a maximum lignin reduction	970:995	a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 	970:1311	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	3	60	theme	24.70	752:756	arg1	ratio					743:747	C/N ratio	739:747	C/N ratio of 24.70	739:756	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	3	61	dep	mud	849:851	arg1	comparison					828:837	comparison	828:837	comparison	828:837	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	1	62	theme	degradation	133:143	arg1	codigestion					118:128	the codigestion	114:128	the codigestion of degradation	114:143	To enhance the codigestion of degradation and improve biomethane production potential, sugarcane bagasse and filter mud were pretreated by sodium hydroxide NaOH 1 N at 100°C for 15, 30, and 45 minutes, respectively.
27738635	3	63	theme	filter	790:795	arg1	mud					797:799	filter mud	790:799	filter mud	790:799	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	4	64	from	increase	1351:1358	arg1	composition					1385:1395	untreated composition	1375:1395	untreated composition	1375:1395	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	3	65	theme	filter	558:563	arg1	mud					565:567	filter mud	558:567	filter mud	558:567	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	4	66	dep	biomethane	1031:1040	arg1	195.8 mL·gVS-1					1043:1056	195.8 mL·gVS-1	1043:1056	195.8 mL·gVS-1	1043:1056	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	2	67	theme	batch	354:358	arg1	reactor					360:366	1-liter batch reactor	346:366	1-liter batch reactor	346:366	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	1	68	theme	sodium	242:247	arg1	1 N					264:266	sodium hydroxide NaOH 1 N	242:266	sodium hydroxide NaOH 1 N	242:266	To enhance the codigestion of degradation and improve biomethane production potential, sugarcane bagasse and filter mud were pretreated by sodium hydroxide NaOH 1 N at 100°C for 15, 30, and 45 minutes, respectively.
27738635	2	69	theme	solid	418:422	arg1	concentrations					424:437	solid concentrations	418:437	solid concentrations of 6%	418:443	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	4	70	theme	cumulative	1230:1239	arg1	yield					1249:1253	cumulative methane yield	1230:1253	cumulative methane yield	1230:1253	All the pretreatments tested led to solubilization of the organic matter, with a maximum lignin reduction of 86.27% and cumulative yield of biomethane (195.8 mL·gVS-1, digestion of pretreated bagasse as single substrate) obtained after 45 minutes of cooking by NaOH 1 N at 100°C. Under this pretreatment condition, significant increase in cumulative methane yield was observed (126.2 mL·gVS-1) at codigestion ratio of 25 : 75 between filter mud and bagasse by increase of 81.20% from untreated composition.
27738635	3	71	theme	mud	565:567	arg1	codigestion					543:553	codigestion	543:553	codigestion of filter mud with bagasse	543:580	The results demonstrate that codigestion of filter mud with bagasse produces more biomethane than fermentation of filter mud as single substrate; even codigested substrate composition presented a better balance of nutrients (C/N ratio of 24.70) when codigestion ratio between filter mud and bagasse was 25 : 75 in comparison to filter mud as single substrate (C/N ratio 9.68).
27738635	2	72	theme	1-liter	346:352	arg1	reactor					360:366	1-liter batch reactor	346:366	1-liter batch reactor	346:366	Biomethane generation from 1-liter batch reactor was studied at mesophilic temperature (37 ± 1)°C, solid concentrations of 6%, and five levels of mixing proportion with and without pretreatment.
27738635	1	73	theme	hydroxide	249:257	arg1	1 N					264:266	sodium hydroxide NaOH 1 N	242:266	sodium hydroxide NaOH 1 N	242:266	To enhance the codigestion of degradation and improve biomethane production potential, sugarcane bagasse and filter mud were pretreated by sodium hydroxide NaOH 1 N at 100°C for 15, 30, and 45 minutes, respectively.
25465557	7	0	dep	preparations	1125:1136	arg1	protein					1160:1166	approximately 61.40% protein	1139:1166	approximately 61.40% protein	1139:1166	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	7	0	dep	preparations	1125:1136	arg1	preparations					1125:1136	the 2 MPC preparations	1115:1136	the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose	1115:1185	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	7	0	dep	preparations	1125:1136	arg1	lactose					1179:1185	28.49% lactose	1172:1185	28.49% lactose	1172:1185	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	7	1	theme	%	1177:1177	arg1	preparations					1125:1136	the 2 MPC preparations	1115:1136	the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose	1115:1185	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	7	1	theme	%	1177:1177	arg1	lactose					1179:1185	28.49% lactose	1172:1185	28.49% lactose	1172:1185	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	2	2	theme	milk	360:363	arg1	samples					459:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	1	3	theme	concentration	242:254	arg1	technologies					256:267	concentration technologies	242:267	concentration technologies	242:267	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
25465557	0	4	theme	physiochemical	70:83	arg1	properties					85:94	the physiochemical properties	66:94	the physiochemical properties of milk protein during processing of milk protein	66:144	Short communication: Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein concentrate.
25465557	2	5	theme	Skim	355:358	arg1	milk					360:363	Skim milk	355:363	Skim milk	355:363	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	9	6	dep	higher	1336:1341	arg1	0.58mL					1328:1333	0.58mL	1328:1333	0.58mL	1328:1333	However, the insolubility index of evaporated MPC was significantly (0.58mL) higher than that of nanofiltered MPC.
25465557	9	7	theme	insolubility	1272:1283	arg1	higher					1336:1341	higher	1336:1341	higher	1336:1341	However, the insolubility index of evaporated MPC was significantly (0.58mL) higher than that of nanofiltered MPC.
25465557	9	7	theme	insolubility	1272:1283	arg1	index					1285:1289	the insolubility index	1268:1289	the insolubility index of evaporated MPC	1268:1307	However, the insolubility index of evaporated MPC was significantly (0.58mL) higher than that of nanofiltered MPC.
25465557	0	8	theme	milk	99:102	arg1	protein					104:110	milk protein	99:110	milk protein	99:110	Short communication: Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein concentrate.
25465557	3	9	theme	Chemical	514:521	arg1	composition					523:533	Chemical composition	514:533	Chemical composition	514:533	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	7	10	located	found	1077:1081	arg1	composition					1095:1105	chemical composition	1086:1105	chemical composition	1086:1105	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	7	10	located	found	1077:1081	arg2	difference					1062:1071	No significant difference	1047:1071	No significant difference	1047:1071	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	7	11	theme	28.49	1172:1176	arg1	%					1177:1177	%	1177:1177	%	1177:1177	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	3	12	theme	casein	554:559	arg1	micelles					561:568	casein micelles	554:568	casein micelles	554:568	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	6	13	theme	micelles	924:931	arg1	microstructure					895:908	the microstructure	891:908	the microstructure of the casein micelles	891:931	However, the microstructure of the casein micelles was relatively undisturbed after nanofiltration, with reduced surface hydrophobicity and free sulfhydryl content.
25465557	4	14	theme	MPC	681:683	arg1	index					672:676	The insolubility index	655:676	The insolubility index of MPC	655:683	The insolubility index of MPC was also determined.
25465557	2	15	theme	milk	397:400	arg1	samples					459:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	0	16	theme	protein	104:110	arg1	properties					85:94	the physiochemical properties	66:94	the physiochemical properties of milk protein during processing of milk protein	66:144	Short communication: Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein concentrate.
25465557	6	17	theme	casein	917:922	arg1	micelles					924:931	the casein micelles	913:931	the casein micelles	913:931	However, the microstructure of the casein micelles was relatively undisturbed after nanofiltration, with reduced surface hydrophobicity and free sulfhydryl content.
25465557	5	18	theme	sulfhydryl	809:818	arg1	content					820:826	free sulfhydryl content	804:826	free sulfhydryl content	804:826	Casein micelles aggregated compactly after evaporation while surface hydrophobicity increased and free sulfhydryl content decreased in evaporated milk compared with skim milk.
25465557	7	19	dep	%	1158:1158	arg1	61.40					1153:1157	61.40	1153:1157	61.40	1153:1157	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	2	20	theme	evaporated	386:395	arg1	milk					397:400	evaporated milk	386:400	evaporated milk	386:400	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	5	21	theme	evaporated	841:850	arg1	milk					852:855	evaporated milk	841:855	evaporated milk	841:855	Casein micelles aggregated compactly after evaporation while surface hydrophobicity increased and free sulfhydryl content decreased in evaporated milk compared with skim milk.
25465557	5	22	theme	free	804:807	arg1	content					820:826	free sulfhydryl content	804:826	free sulfhydryl content	804:826	Casein micelles aggregated compactly after evaporation while surface hydrophobicity increased and free sulfhydryl content decreased in evaporated milk compared with skim milk.
25465557	6	23	theme	sulfhydryl	1027:1036	arg1	content					1038:1044	free sulfhydryl content	1022:1044	free sulfhydryl content	1022:1044	However, the microstructure of the casein micelles was relatively undisturbed after nanofiltration, with reduced surface hydrophobicity and free sulfhydryl content.
25465557	3	24	theme	free	571:574	arg1	content					587:593	free sulfhydryl content	571:593	free sulfhydryl content	571:593	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	1	25	theme	work	175:178	arg1	aim					163:165	The aim	159:165	The aim of this work	159:178	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
25465557	0	26	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein concentrate.	0:157	Short communication: Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein concentrate.
25465557	1	27	theme	physiochemical	276:289	arg1	properties					291:300	the physiochemical properties	272:300	the physiochemical properties of milk protein concentrate (MPC)	272:334	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
25465557	10	28	theme	products	1446:1453	arg1	solubility					1428:1437	the solubility	1424:1437	the solubility of MPC products	1424:1453	Nanofiltration may be an effective way to improve the solubility of MPC products.
25465557	3	29	theme	sulfhydryl	576:585	arg1	content					587:593	free sulfhydryl content	571:593	free sulfhydryl content	571:593	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	2	30	theme	milk	380:383	arg1	samples					459:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	2	31	theme	MPC	433:435	arg1	samples					459:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	6	32	theme	free	1022:1025	arg1	content					1038:1044	free sulfhydryl content	1022:1044	free sulfhydryl content	1022:1044	However, the microstructure of the casein micelles was relatively undisturbed after nanofiltration, with reduced surface hydrophobicity and free sulfhydryl content.
25465557	5	33	theme	Casein	706:711	arg1	micelles					713:720	Casein micelles	706:720	Casein micelles	706:720	Casein micelles aggregated compactly after evaporation while surface hydrophobicity increased and free sulfhydryl content decreased in evaporated milk compared with skim milk.
25465557	10	34	theme	MPC	1442:1444	arg1	products					1446:1453	MPC products	1442:1453	MPC products	1442:1453	Nanofiltration may be an effective way to improve the solubility of MPC products.
25465557	3	35	theme	micelles	561:568	arg1	content					587:593	free sulfhydryl content	571:593	free sulfhydryl content	571:593	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	3	35	theme	micelles	561:568	arg1	microstructure					536:549	microstructure	536:549	microstructure of casein micelles	536:568	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	3	35	theme	micelles	561:568	arg1	hydrophobicity					608:621	surface hydrophobicity	600:621	surface hydrophobicity of the samples	600:636	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	3	35	theme	micelles	561:568	arg1	composition					523:533	Chemical composition	514:533	Chemical composition	514:533	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	5	36	theme	skim	871:874	arg1	milk					876:879	skim milk	871:879	skim milk	871:879	Casein micelles aggregated compactly after evaporation while surface hydrophobicity increased and free sulfhydryl content decreased in evaporated milk compared with skim milk.
25465557	2	37	theme	evaporated	422:431	arg1	MPC					433:435	evaporated MPC	422:435	evaporated MPC	422:435	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	7	38	theme	chemical	1086:1093	arg1	composition					1095:1105	chemical composition	1086:1105	chemical composition	1086:1105	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	7	39	dep	protein	1160:1166	arg1	%					1158:1158	%	1158:1158	%	1158:1158	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	2	40	theme	milk	416:419	arg1	samples					459:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	0	41	theme	nanofiltration	32:45	arg1	Effects					21:27	Effects	21:27	Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein	21:144	Short communication: Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein concentrate.
25465557	1	42	theme	milk	305:308	arg1	concentrate					318:328	milk protein concentrate	305:328	milk protein concentrate (MPC)	305:334	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
25465557	1	42	theme	milk	305:308	arg1	MPC					331:333	MPC	331:333	MPC	331:333	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
25465557	0	43	theme	protein	138:144	arg1	processing					119:128	processing	119:128	processing of milk protein	119:144	Short communication: Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein concentrate.
25465557	8	44	from	addition	1191:1198	arg1	similar					1250:1256	similar	1250:1256	similar	1250:1256	In addition, the particulate microstructures of both MPC were similar.
25465557	8	44	from	addition	1191:1198	arg1	microstructures					1217:1231	the particulate microstructures	1201:1231	the particulate microstructures of both MPC	1201:1243	In addition, the particulate microstructures of both MPC were similar.
25465557	2	45	theme	nanofiltered	403:414	arg1	milk					416:419	nanofiltered milk	403:419	nanofiltered milk	403:419	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	1	46	theme	protein	310:316	arg1	concentrate					318:328	milk protein concentrate	305:328	milk protein concentrate (MPC)	305:334	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
25465557	1	46	theme	protein	310:316	arg1	MPC					331:333	MPC	331:333	MPC	331:333	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
25465557	0	47	theme	milk	133:136	arg1	protein					138:144	milk protein	133:144	milk protein	133:144	Short communication: Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein concentrate.
25465557	3	48	theme	surface	600:606	arg1	hydrophobicity					608:621	surface hydrophobicity	600:621	surface hydrophobicity of the samples	600:636	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	5	49	theme	surface	767:773	arg1	hydrophobicity					775:788	surface hydrophobicity	767:788	surface hydrophobicity	767:788	Casein micelles aggregated compactly after evaporation while surface hydrophobicity increased and free sulfhydryl content decreased in evaporated milk compared with skim milk.
25465557	0	50	theme	evaporation	51:61	arg1	Effects					21:27	Effects	21:27	Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein	21:144	Short communication: Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein concentrate.
25465557	1	51	theme	concentrate	318:328	arg1	properties					291:300	the physiochemical properties	272:300	the physiochemical properties of milk protein concentrate (MPC)	272:334	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
25465557	1	52	dep	nanofiltration	211:224	arg1	technologies					256:267	concentration technologies	242:267	concentration technologies	242:267	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
25465557	1	53	from	effects	200:206	arg1	properties					291:300	the physiochemical properties	272:300	the physiochemical properties of milk protein concentrate (MPC)	272:334	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
25465557	7	54	theme	MPC	1121:1123	arg1	protein					1160:1166	approximately 61.40% protein	1139:1166	approximately 61.40% protein	1139:1166	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	7	54	theme	MPC	1121:1123	arg1	preparations					1125:1136	the 2 MPC preparations	1115:1136	the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose	1115:1185	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	7	54	theme	MPC	1121:1123	arg1	lactose					1179:1185	28.49% lactose	1172:1185	28.49% lactose	1172:1185	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	2	55	theme	nanofiltered	442:453	arg1	MPC					455:457	nanofiltered MPC	442:457	nanofiltered MPC	442:457	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	8	56	from	similar	1250:1256	arg1	addition					1191:1198	addition	1191:1198	addition	1191:1198	In addition, the particulate microstructures of both MPC were similar.
25465557	9	57	theme	MPC	1305:1307	arg1	higher					1336:1341	higher	1336:1341	higher	1336:1341	However, the insolubility index of evaporated MPC was significantly (0.58mL) higher than that of nanofiltered MPC.
25465557	9	57	theme	MPC	1305:1307	arg1	index					1285:1289	the insolubility index	1268:1289	the insolubility index of evaporated MPC	1268:1307	However, the insolubility index of evaporated MPC was significantly (0.58mL) higher than that of nanofiltered MPC.
25465557	9	58	theme	evaporated	1294:1303	arg1	MPC					1305:1307	evaporated MPC	1294:1307	evaporated MPC	1294:1307	However, the insolubility index of evaporated MPC was significantly (0.58mL) higher than that of nanofiltered MPC.
25465557	9	59	theme	nanofiltered	1356:1367	arg1	MPC					1369:1371	nanofiltered MPC	1356:1371	nanofiltered MPC	1356:1371	However, the insolubility index of evaporated MPC was significantly (0.58mL) higher than that of nanofiltered MPC.
25465557	6	60	theme	surface	995:1001	arg1	hydrophobicity					1003:1016	reduced surface hydrophobicity	987:1016	reduced surface hydrophobicity	987:1016	However, the microstructure of the casein micelles was relatively undisturbed after nanofiltration, with reduced surface hydrophobicity and free sulfhydryl content.
25465557	8	61	theme	particulate	1205:1215	arg1	similar					1250:1256	similar	1250:1256	similar	1250:1256	In addition, the particulate microstructures of both MPC were similar.
25465557	8	61	theme	particulate	1205:1215	arg1	microstructures					1217:1231	the particulate microstructures	1201:1231	the particulate microstructures of both MPC	1201:1243	In addition, the particulate microstructures of both MPC were similar.
25465557	3	62	theme	samples	630:636	arg1	content					587:593	free sulfhydryl content	571:593	free sulfhydryl content	571:593	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	3	62	theme	samples	630:636	arg1	microstructure					536:549	microstructure	536:549	microstructure of casein micelles	536:568	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	3	62	theme	samples	630:636	arg1	hydrophobicity					608:621	surface hydrophobicity	600:621	surface hydrophobicity of the samples	600:636	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	3	62	theme	samples	630:636	arg1	composition					523:533	Chemical composition	514:533	Chemical composition	514:533	Chemical composition, microstructure of casein micelles, free sulfhydryl content, and surface hydrophobicity of the samples were determined.
25465557	2	63	theme	different	485:493	arg1	stages					506:511	different processing stages	485:511	different processing stages	485:511	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	1	64	theme	nanofiltration	211:224	arg1	effects					200:206	the effects	196:206	the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC)	196:334	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
25465557	2	65	theme	processing	495:504	arg1	stages					506:511	different processing stages	485:511	different processing stages	485:511	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	0	66	dep	communication	6:18	arg1	concentrate					146:156	concentrate	146:156	concentrate	146:156	Short communication: Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein concentrate.
25465557	7	67	theme	significant	1050:1060	arg1	difference					1062:1071	No significant difference	1047:1071	No significant difference	1047:1071	No significant difference was found in chemical composition between the 2 MPC preparations: approximately 61.40% protein and 28.49% lactose.
25465557	2	68	theme	ultrafiltered	366:378	arg1	milk					380:383	ultrafiltered milk	366:383	ultrafiltered milk	366:383	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	4	69	theme	insolubility	659:670	arg1	index					672:676	The insolubility index	655:676	The insolubility index of MPC	655:683	The insolubility index of MPC was also determined.
25465557	2	70	theme	MPC	455:457	arg1	samples					459:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples	355:465	Skim milk, ultrafiltered milk, evaporated milk, nanofiltered milk, evaporated MPC, and nanofiltered MPC samples were collected at different processing stages.
25465557	0	71	from	Effects	21:27	arg1	properties					85:94	the physiochemical properties	66:94	the physiochemical properties of milk protein during processing of milk protein	66:144	Short communication: Effects of nanofiltration and evaporation on the physiochemical properties of milk protein during processing of milk protein concentrate.
25465557	10	72	theme	effective	1399:1407	arg1	way					1409:1411	an effective way	1396:1411	an effective way to improve the solubility of MPC products	1396:1453	Nanofiltration may be an effective way to improve the solubility of MPC products.
25465557	10	72	theme	effective	1399:1407	arg1	Nanofiltration					1374:1387	Nanofiltration	1374:1387	Nanofiltration	1374:1387	Nanofiltration may be an effective way to improve the solubility of MPC products.
25465557	8	73	theme	MPC	1241:1243	arg1	similar					1250:1256	similar	1250:1256	similar	1250:1256	In addition, the particulate microstructures of both MPC were similar.
25465557	8	73	theme	MPC	1241:1243	arg1	microstructures					1217:1231	the particulate microstructures	1201:1231	the particulate microstructures of both MPC	1201:1243	In addition, the particulate microstructures of both MPC were similar.
25465557	6	74	theme	reduced	987:993	arg1	hydrophobicity					1003:1016	reduced surface hydrophobicity	987:1016	reduced surface hydrophobicity	987:1016	However, the microstructure of the casein micelles was relatively undisturbed after nanofiltration, with reduced surface hydrophobicity and free sulfhydryl content.
25465557	1	75	theme	evaporation	230:240	arg1	effects					200:206	the effects	196:206	the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC)	196:334	The aim of this work was to evaluate the effects of nanofiltration and evaporation concentration technologies on the physiochemical properties of milk protein concentrate (MPC) during processing.
26869786	6	0	from	studies	1001:1007	arg1	Results					939:945	Results	939:945	Results from real-time quantitative polymerase chain reaction studies	939:1007	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	6	1	theme	reaction	992:999	arg1	studies					1001:1007	real-time quantitative polymerase chain reaction studies	952:1007	real-time quantitative polymerase chain reaction studies	952:1007	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	4	2	theme	anodized	754:761	arg1	surfaces					777:784	The anodized TiO2 nanotube surfaces	750:784	The anodized TiO2 nanotube surfaces	750:784	The anodized TiO2 nanotube surfaces exhibited an amorphous glassy matrix perpendicular to the Ti surface.
26869786	2	3	theme	nanotube	404:411	arg1	coatings					413:420	Lenti-BMP-2-loaded TiO2 nanotube coatings	380:420	Lenti-BMP-2-loaded TiO2 nanotube coatings	380:420	In this paper, Lenti-BMP-2-loaded TiO2 nanotube coatings were fabricated by lyophilization in the presence of trehalose to functionalize the surface.
26869786	0	4	theme	layers	98:103	arg1	activity					20:27	Enhanced osteogenic activity	0:27	Enhanced osteogenic activity	0:27	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO₂ nanotube layers fabricated by lyophilization following trehalose addition.
26869786	0	4	theme	layers	98:103	arg1	properties					51:60	anti-inflammatory properties	33:60	anti-inflammatory properties	33:60	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO₂ nanotube layers fabricated by lyophilization following trehalose addition.
26869786	6	5	theme	chain	986:990	arg1	reaction					992:999	real-time quantitative polymerase chain reaction	952:999	real-time quantitative polymerase chain reaction studies	952:1007	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	2	6	theme	TiO2	399:402	arg1	coatings					413:420	Lenti-BMP-2-loaded TiO2 nanotube coatings	380:420	Lenti-BMP-2-loaded TiO2 nanotube coatings	380:420	In this paper, Lenti-BMP-2-loaded TiO2 nanotube coatings were fabricated by lyophilization in the presence of trehalose to functionalize the surface.
26869786	6	7	theme	polymerase	975:984	arg1	reaction					992:999	real-time quantitative polymerase chain reaction	952:999	real-time quantitative polymerase chain reaction studies	952:1007	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	7	8	theme	Lyo-Tre-Lenti-BMP-2	1236:1254	arg1	nanotubes					1256:1264	Lyo-Tre-Lenti-BMP-2 nanotubes	1236:1264	Lyo-Tre-Lenti-BMP-2 nanotubes	1236:1264	In addition, Lyo-Tre-Lenti-BMP-2 nanotubes effectively inhibited lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production.
26869786	6	9	theme	quantitative	962:973	arg1	reaction					992:999	real-time quantitative polymerase chain reaction	952:999	real-time quantitative polymerase chain reaction studies	952:1007	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	3	10	theme	Lenti-BMP-2	600:610	arg1	terms					556:560	terms	556:560	terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration	556:739	We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo.
26869786	1	11	theme	surface	314:320	arg1	topography					322:331	the surface topography	310:331	the surface topography	310:331	To enhance biocompatibility and osseointegration between titanium implants and surrounding bone tissue, numerous efforts have been made to modify the surface topography and composition of Ti implants.
26869786	0	12	theme	trehalose	144:152	arg1	addition					154:161	trehalose addition	144:161	trehalose addition	144:161	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO₂ nanotube layers fabricated by lyophilization following trehalose addition.
26869786	4	13	theme	nanotube	768:775	arg1	surfaces					777:784	The anodized TiO2 nanotube surfaces	750:784	The anodized TiO2 nanotube surfaces	750:784	The anodized TiO2 nanotube surfaces exhibited an amorphous glassy matrix perpendicular to the Ti surface.
26869786	6	14	theme	stromal	1180:1186	arg1	cells					1188:1192	bone marrow stromal cells	1168:1192	bone marrow stromal cells	1168:1192	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	3	15	theme	surface	580:586	arg1	morphology					588:597	surface morphology	580:597	surface morphology	580:597	We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo.
26869786	1	16	theme	titanium	221:228	arg1	implants					230:237	titanium implants	221:237	titanium implants	221:237	To enhance biocompatibility and osseointegration between titanium implants and surrounding bone tissue, numerous efforts have been made to modify the surface topography and composition of Ti implants.
26869786	7	17	theme	lipopolysaccharide-induced	1288:1313	arg1	interleukin-1β					1315:1328	lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production	1288:1367	interleukin-1β	1315:1328	In addition, Lyo-Tre-Lenti-BMP-2 nanotubes effectively inhibited lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production.
26869786	3	18	theme	morphology	588:597	arg1	terms					556:560	terms	556:560	terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration	556:739	We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo.
26869786	0	19	theme	osteogenic	9:18	arg1	activity					20:27	Enhanced osteogenic activity	0:27	Enhanced osteogenic activity	0:27	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO₂ nanotube layers fabricated by lyophilization following trehalose addition.
26869786	4	20	theme	amorphous	799:807	arg1	perpendicular					823:835	an amorphous glassy matrix perpendicular	796:835	an amorphous glassy matrix perpendicular to the Ti surface	796:853	The anodized TiO2 nanotube surfaces exhibited an amorphous glassy matrix perpendicular to the Ti surface.
26869786	6	21	theme	real-time	952:960	arg1	reaction					992:999	real-time quantitative polymerase chain reaction	952:999	real-time quantitative polymerase chain reaction studies	952:1007	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	5	22	theme	days	933:936	arg1	course					921:926	the course	917:926	the course of 8 days	917:936	Both Lenti-BMP-2 and trehalose showed sustained release over the course of 8 days.
26869786	0	23	theme	Enhanced	0:7	arg1	activity					20:27	Enhanced osteogenic activity	0:27	Enhanced osteogenic activity	0:27	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO₂ nanotube layers fabricated by lyophilization following trehalose addition.
26869786	1	24	theme	surrounding	243:253	arg1	tissue					260:265	surrounding bone tissue	243:265	surrounding bone tissue	243:265	To enhance biocompatibility and osseointegration between titanium implants and surrounding bone tissue, numerous efforts have been made to modify the surface topography and composition of Ti implants.
26869786	7	25	theme	necrosis	1340:1347	arg1	production					1358:1367	lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production	1288:1367	production	1358:1367	In addition, Lyo-Tre-Lenti-BMP-2 nanotubes effectively inhibited lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production.
26869786	9	26	theme	osteogenic	1553:1562	arg1	potential					1564:1572	their osteogenic potential	1547:1572	their osteogenic potential	1547:1572	This study presents a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs.
26869786	4	27	theme	matrix	816:821	arg1	perpendicular					823:835	an amorphous glassy matrix perpendicular	796:835	an amorphous glassy matrix perpendicular to the Ti surface	796:853	The anodized TiO2 nanotube surfaces exhibited an amorphous glassy matrix perpendicular to the Ti surface.
26869786	2	28	theme	trehalose	475:483	arg1	presence					463:470	the presence	459:470	the presence of trehalose to functionalize the surface	459:512	In this paper, Lenti-BMP-2-loaded TiO2 nanotube coatings were fabricated by lyophilization in the presence of trehalose to functionalize the surface.
26869786	5	29	theme	sustained	894:902	arg1	release					904:910	sustained release	894:910	sustained release	894:910	Both Lenti-BMP-2 and trehalose showed sustained release over the course of 8 days.
26869786	4	30	theme	Ti	844:845	arg1	surface					847:853	the Ti surface	840:853	the Ti surface	840:853	The anodized TiO2 nanotube surfaces exhibited an amorphous glassy matrix perpendicular to the Ti surface.
26869786	4	31	theme	glassy	809:814	arg1	perpendicular					823:835	an amorphous glassy matrix perpendicular	796:835	an amorphous glassy matrix perpendicular to the Ti surface	796:853	The anodized TiO2 nanotube surfaces exhibited an amorphous glassy matrix perpendicular to the Ti surface.
26869786	3	32	theme	TiO2	532:535	arg1	layers					546:551	TiO2 nanotube layers	532:551	TiO2 nanotube layers	532:551	We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo.
26869786	9	33	dep	clinical	1633:1640	arg1	needs					1642:1646	needs	1642:1646	needs	1642:1646	This study presents a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs.
26869786	6	34	theme	marrow	1173:1178	arg1	cells					1188:1192	bone marrow stromal cells	1168:1192	bone marrow stromal cells	1168:1192	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	0	35	theme	anti-inflammatory	33:49	arg1	properties					51:60	anti-inflammatory properties	33:60	anti-inflammatory properties	33:60	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO₂ nanotube layers fabricated by lyophilization following trehalose addition.
26869786	1	36	theme	Ti	352:353	arg1	implants					355:362	Ti implants	352:362	Ti implants	352:362	To enhance biocompatibility and osseointegration between titanium implants and surrounding bone tissue, numerous efforts have been made to modify the surface topography and composition of Ti implants.
26869786	7	37	theme	tumor	1334:1338	arg1	production					1358:1367	lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production	1288:1367	production	1358:1367	In addition, Lyo-Tre-Lenti-BMP-2 nanotubes effectively inhibited lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production.
26869786	1	38	theme	numerous	268:275	arg1	efforts					277:283	numerous efforts	268:283	numerous efforts	268:283	To enhance biocompatibility and osseointegration between titanium implants and surrounding bone tissue, numerous efforts have been made to modify the surface topography and composition of Ti implants.
26869786	0	39	theme	Lenti-BMP-2-loaded	65:82	arg1	layers					98:103	Lenti-BMP-2-loaded TiO₂ nanotube layers	65:103	Lenti-BMP-2-loaded TiO₂ nanotube layers	65:103	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO₂ nanotube layers fabricated by lyophilization following trehalose addition.
26869786	1	40	theme	implants	355:362	arg1	composition					337:347	composition	337:347	composition	337:347	To enhance biocompatibility and osseointegration between titanium implants and surrounding bone tissue, numerous efforts have been made to modify the surface topography and composition of Ti implants.
26869786	1	40	theme	implants	355:362	arg1	topography					322:331	the surface topography	310:331	the surface topography	310:331	To enhance biocompatibility and osseointegration between titanium implants and surrounding bone tissue, numerous efforts have been made to modify the surface topography and composition of Ti implants.
26869786	2	41	theme	Lenti-BMP-2-loaded	380:397	arg1	coatings					413:420	Lenti-BMP-2-loaded TiO2 nanotube coatings	380:420	Lenti-BMP-2-loaded TiO2 nanotube coatings	380:420	In this paper, Lenti-BMP-2-loaded TiO2 nanotube coatings were fabricated by lyophilization in the presence of trehalose to functionalize the surface.
26869786	9	42	theme	Ti-based	1503:1510	arg1	implants					1512:1519	biomedical Ti-based implants	1492:1519	biomedical Ti-based implants	1492:1519	This study presents a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs.
26869786	3	43	theme	osseointegration	724:739	arg1	terms					556:560	terms	556:560	terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration	556:739	We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo.
26869786	3	44	theme	ability	641:647	arg1	terms					556:560	terms	556:560	terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration	556:739	We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo.
26869786	4	45	theme	TiO2	763:766	arg1	surfaces					777:784	The anodized TiO2 nanotube surfaces	750:784	The anodized TiO2 nanotube surfaces	750:784	The anodized TiO2 nanotube surfaces exhibited an amorphous glassy matrix perpendicular to the Ti surface.
26869786	6	46	theme	Lenti-BMP-2/TiO2	1039:1054	arg1	nanotubes					1056:1064	lyophilized Lenti-BMP-2/TiO2 nanotubes	1027:1064	lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2)	1027:1113	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	9	47	theme	promising	1449:1457	arg1	method					1463:1468	a promising new method	1447:1468	a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs	1447:1646	This study presents a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs.
26869786	3	48	theme	nanotube	537:544	arg1	layers					546:551	TiO2 nanotube layers	532:551	TiO2 nanotube layers	532:551	We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo.
26869786	9	49	theme	new	1459:1461	arg1	method					1463:1468	a promising new method	1447:1468	a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs	1447:1646	This study presents a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs.
26869786	6	50	theme	cells	1188:1192	arg1	differentiation					1149:1163	osteogenic differentiation	1138:1163	osteogenic differentiation of bone marrow stromal cells but not their proliferation	1138:1220	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	3	51	dep	Lenti-BMP-2	600:610	arg1	release					626:632	release	626:632	release	626:632	We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo.
26869786	9	52	theme	anti-inflammatory	1578:1594	arg1	properties					1596:1605	anti-inflammatory properties	1578:1605	anti-inflammatory properties	1578:1605	This study presents a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs.
26869786	0	53	theme	nanotube	89:96	arg1	layers					98:103	Lenti-BMP-2-loaded TiO₂ nanotube layers	65:103	Lenti-BMP-2-loaded TiO₂ nanotube layers	65:103	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO₂ nanotube layers fabricated by lyophilization following trehalose addition.
26869786	6	54	theme	bone	1168:1171	arg1	cells					1188:1192	bone marrow stromal cells	1168:1192	bone marrow stromal cells	1168:1192	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	1	55	theme	bone	255:258	arg1	tissue					260:265	surrounding bone tissue	243:265	surrounding bone tissue	243:265	To enhance biocompatibility and osseointegration between titanium implants and surrounding bone tissue, numerous efforts have been made to modify the surface topography and composition of Ti implants.
26869786	3	56	theme	following	569:577	arg1	terms					556:560	terms	556:560	terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration	556:739	We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo.
26869786	6	57	theme	osteogenic	1138:1147	arg1	differentiation					1149:1163	osteogenic differentiation	1138:1163	osteogenic differentiation of bone marrow stromal cells but not their proliferation	1138:1220	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
26869786	0	58	theme	TiO₂	84:87	arg1	layers					98:103	Lenti-BMP-2-loaded TiO₂ nanotube layers	65:103	Lenti-BMP-2-loaded TiO₂ nanotube layers	65:103	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO₂ nanotube layers fabricated by lyophilization following trehalose addition.
26869786	9	59	theme	biomedical	1492:1501	arg1	implants					1512:1519	biomedical Ti-based implants	1492:1519	biomedical Ti-based implants	1492:1519	This study presents a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs.
26869786	7	60	theme	factor-α	1349:1356	arg1	production					1358:1367	lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production	1288:1367	production	1358:1367	In addition, Lyo-Tre-Lenti-BMP-2 nanotubes effectively inhibited lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production.
26869786	6	61	theme	lyophilized	1027:1037	arg1	nanotubes					1056:1064	lyophilized Lenti-BMP-2/TiO2 nanotubes	1027:1064	lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2)	1027:1113	Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation.
24534508	7	0	theme	N	1093:1093	arg1	flow					1095:1098	microbial N flow	1083:1098	microbial N flow to the duodenum	1083:1114	The amylase supplement also increased the true ruminal digestibility of organic matter but did not affect microbial N flow to the duodenum.
24534508	2	1	theme	Latin	326:330	arg1	design					339:344	a 4×4 Latin square design	320:344	a 4×4 Latin square design	320:344	Rumen and total-tract nutrient digestibility were measured in a 4×4 Latin square design with 28-d periods using 4 first-lactation cows cannulated at the rumen and duodenum.
24534508	1	2	theme	diets	235:239	arg1	digestion					201:209	digestion	201:209	digestion of low- and high-starch diets in dairy cattle	201:255	The objective of this study was to evaluate the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle.
24534508	8	3	theme	Amylase	1117:1123	arg1	supplement					1125:1134	Amylase supplement	1117:1134	Amylase supplement	1117:1134	Amylase supplement reduced the proportion of acetate and butyrate and increased that of propionate, particularly in the high-starch diet, where it tended to increase the concentration of total volatile fatty acids in the rumen.
24534508	1	4	theme	dairy	244:248	arg1	cattle					250:255	dairy cattle	244:255	dairy cattle	244:255	The objective of this study was to evaluate the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle.
24534508	11	5	theme	starch	1810:1815	arg1	digestion					1817:1825	starch digestion	1810:1825	starch digestion	1810:1825	We conclude that the exogenous amylase improved starch digestion in the rumen in first-lactation cows with moderate intake and production levels.
24534508	4	6	theme	additive	614:621	arg1	enzyme					607:612	the enzyme additive	603:621	the enzyme additive	603:621	Effects of the enzyme additive were observed on ruminal digestibility but not at the total-tract level.
24534508	5	7	with	%	763:763	arg1	supplementation					778:792	amylase supplementation	770:792	amylase supplementation	770:792	Ruminal digestibility of starch increased from 75% in control to 81% with amylase supplementation.
24534508	6	8	from	difference	800:809	arg1	digestion					829:837	ruminal starch digestion	814:837	ruminal starch digestion	814:837	This difference in ruminal starch digestion was compensated postruminally, so that the total-tract digestibility of starch was almost complete and did not differ between treatments.
24534508	7	9	theme	matter	1057:1062	arg1	digestibility					1032:1044	the true ruminal digestibility	1015:1044	the true ruminal digestibility of organic matter	1015:1062	The amylase supplement also increased the true ruminal digestibility of organic matter but did not affect microbial N flow to the duodenum.
24534508	9	10	theme	solid-associated	1397:1412	arg1	community					1424:1432	the solid-associated microbial community	1393:1432	the solid-associated microbial community	1393:1432	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	8	11	theme	acetate	1162:1168	arg1	proportion					1148:1157	the proportion	1144:1157	the proportion of acetate and butyrate	1144:1181	Amylase supplement reduced the proportion of acetate and butyrate and increased that of propionate, particularly in the high-starch diet, where it tended to increase the concentration of total volatile fatty acids in the rumen.
24534508	10	12	dep	digestibility	1611:1623	arg1	fiber					1579:1583	neutral detergent fiber	1561:1583	neutral detergent fiber	1561:1583	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	10	12	dep	digestibility	1611:1623	arg1	fiber					1604:1608	acid detergent fiber	1589:1608	acid detergent fiber	1589:1608	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	3	13	theme	concentrate	517:527	arg1	composition					502:512	the composition	498:512	the composition of concentrate	498:527	Corn silage-based diets had 20 or 30% starch, attained by changing the composition of concentrate, with or without addition of an exogenous amylase preparation.
24534508	1	14	theme	amylase	178:184	arg1	preparation					186:196	an exogenous amylase preparation	165:196	an exogenous amylase preparation	165:196	The objective of this study was to evaluate the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle.
24534508	8	15	theme	butyrate	1174:1181	arg1	proportion					1148:1157	the proportion	1144:1157	the proportion of acetate and butyrate	1144:1181	Amylase supplement reduced the proportion of acetate and butyrate and increased that of propionate, particularly in the high-starch diet, where it tended to increase the concentration of total volatile fatty acids in the rumen.
24534508	2	16	theme	28-d	351:354	arg1	periods					356:362	28-d periods	351:362	28-d periods using 4 first-lactation cows cannulated at the rumen and duodenum	351:428	Rumen and total-tract nutrient digestibility were measured in a 4×4 Latin square design with 28-d periods using 4 first-lactation cows cannulated at the rumen and duodenum.
24534508	8	17	theme	high-starch	1237:1247	arg1	diet					1249:1252	the high-starch diet	1233:1252	the high-starch diet	1233:1252	Amylase supplement reduced the proportion of acetate and butyrate and increased that of propionate, particularly in the high-starch diet, where it tended to increase the concentration of total volatile fatty acids in the rumen.
24534508	10	18	theme	fiber	1554:1558	arg1	digestibility					1611:1623	dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility	1539:1623	digestibility	1611:1623	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	7	19	theme	ruminal	1024:1030	arg1	digestibility					1032:1044	the true ruminal digestibility	1015:1044	the true ruminal digestibility of organic matter	1015:1062	The amylase supplement also increased the true ruminal digestibility of organic matter but did not affect microbial N flow to the duodenum.
24534508	4	20	located	observed	628:635	arg1	digestibility					648:660	ruminal digestibility	640:660	ruminal digestibility	640:660	Effects of the enzyme additive were observed on ruminal digestibility but not at the total-tract level.
24534508	4	20	located	observed	628:635	arg2	Effects					592:598	Effects	592:598	Effects of the enzyme additive	592:621	Effects of the enzyme additive were observed on ruminal digestibility but not at the total-tract level.
24534508	11	21	theme	moderate	1869:1876	arg1	intake					1878:1883	moderate intake	1869:1883	moderate intake	1869:1883	We conclude that the exogenous amylase improved starch digestion in the rumen in first-lactation cows with moderate intake and production levels.
24534508	6	22	theme	total-tract	882:892	arg1	complete					929:936	complete	929:936	complete	929:936	This difference in ruminal starch digestion was compensated postruminally, so that the total-tract digestibility of starch was almost complete and did not differ between treatments.
24534508	6	22	theme	total-tract	882:892	arg1	digestibility					894:906	the total-tract digestibility	878:906	the total-tract digestibility of starch	878:916	This difference in ruminal starch digestion was compensated postruminally, so that the total-tract digestibility of starch was almost complete and did not differ between treatments.
24534508	4	23	theme	total-tract	677:687	arg1	level					689:693	the total-tract level	673:693	the total-tract level	673:693	Effects of the enzyme additive were observed on ruminal digestibility but not at the total-tract level.
24534508	3	24	theme	preparation	579:589	arg1	addition					546:553	addition	546:553	addition of an exogenous amylase preparation	546:589	Corn silage-based diets had 20 or 30% starch, attained by changing the composition of concentrate, with or without addition of an exogenous amylase preparation.
24534508	1	25	theme	exogenous	168:176	arg1	preparation					186:196	an exogenous amylase preparation	165:196	an exogenous amylase preparation	165:196	The objective of this study was to evaluate the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle.
24534508	11	26	theme	first-lactation	1843:1857	arg1	cows					1859:1862	first-lactation cows	1843:1862	first-lactation cows with moderate intake and production levels	1843:1905	We conclude that the exogenous amylase improved starch digestion in the rumen in first-lactation cows with moderate intake and production levels.
24534508	4	27	theme	ruminal	640:646	arg1	digestibility					648:660	ruminal digestibility	640:660	ruminal digestibility	640:660	Effects of the enzyme additive were observed on ruminal digestibility but not at the total-tract level.
24534508	5	28	theme	starch	721:726	arg1	digestibility					704:716	Ruminal digestibility	696:716	Ruminal digestibility of starch	696:726	Ruminal digestibility of starch increased from 75% in control to 81% with amylase supplementation.
24534508	10	29	theme	microbial	1730:1738	arg1	community					1740:1748	the microbial community	1726:1748	the microbial community in general	1726:1759	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	9	30	theme	protozoa	1470:1477	arg1	numbers					1459:1465	lower numbers	1453:1465	lower numbers of protozoa	1453:1477	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	0	31	theme	high	80:83	arg1	diets					100:104	high and low starch diets	80:104	high and low starch diets	80:104	Amylase addition increases starch ruminal digestion in first-lactation cows fed high and low starch diets.
24534508	0	32	theme	low	89:91	arg1	diets					100:104	high and low starch diets	80:104	high and low starch diets	80:104	Amylase addition increases starch ruminal digestion in first-lactation cows fed high and low starch diets.
24534508	3	33	contain	had	455:457	arg2	starch					469:474	20 or 30% starch	459:474	20 or 30% starch	459:474	Corn silage-based diets had 20 or 30% starch, attained by changing the composition of concentrate, with or without addition of an exogenous amylase preparation.
24534508	3	33	contain	had	455:457	arg1	diets					449:453	Corn silage-based diets	431:453	Corn silage-based diets	431:453	Corn silage-based diets had 20 or 30% starch, attained by changing the composition of concentrate, with or without addition of an exogenous amylase preparation.
24534508	8	34	theme	volatile	1310:1317	arg1	acids					1325:1329	total volatile fatty acids	1304:1329	total volatile fatty acids	1304:1329	Amylase supplement reduced the proportion of acetate and butyrate and increased that of propionate, particularly in the high-starch diet, where it tended to increase the concentration of total volatile fatty acids in the rumen.
24534508	9	35	theme	amylase	1373:1379	arg1	activity					1381:1388	a higher amylase activity	1364:1388	a higher amylase activity in the solid-associated microbial community	1364:1432	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	9	35	theme	amylase	1373:1379	arg1	effects					1351:1357	Other effects	1345:1357	Other effects	1345:1357	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	8	36	theme	acids	1325:1329	arg1	concentration					1287:1299	the concentration	1283:1299	the concentration of total volatile fatty acids in the rumen	1283:1342	Amylase supplement reduced the proportion of acetate and butyrate and increased that of propionate, particularly in the high-starch diet, where it tended to increase the concentration of total volatile fatty acids in the rumen.
24534508	10	37	theme	detergent	1569:1577	arg1	fiber					1579:1583	neutral detergent fiber	1561:1583	neutral detergent fiber	1561:1583	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	0	38	theme	Amylase	0:6	arg1	addition					8:15	Amylase addition	0:15	Amylase addition	0:15	Amylase addition increases starch ruminal digestion in first-lactation cows fed high and low starch diets.
24534508	3	39	dep	starch	469:474	arg1	%					467:467	%	467:467	%	467:467	Corn silage-based diets had 20 or 30% starch, attained by changing the composition of concentrate, with or without addition of an exogenous amylase preparation.
24534508	10	40	from	community	1740:1748	arg1	general					1753:1759	general	1753:1759	general	1753:1759	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	3	41	theme	silage-based	436:447	arg1	diets					449:453	Corn silage-based diets	431:453	Corn silage-based diets	431:453	Corn silage-based diets had 20 or 30% starch, attained by changing the composition of concentrate, with or without addition of an exogenous amylase preparation.
24534508	6	42	theme	starch	822:827	arg1	digestion					829:837	ruminal starch digestion	814:837	ruminal starch digestion	814:837	This difference in ruminal starch digestion was compensated postruminally, so that the total-tract digestibility of starch was almost complete and did not differ between treatments.
24534508	10	43	theme	detergent	1594:1602	arg1	fiber					1604:1608	acid detergent fiber	1589:1608	acid detergent fiber	1589:1608	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	0	44	theme	ruminal	34:40	arg1	digestion					42:50	starch ruminal digestion	27:50	starch ruminal digestion in first-lactation cows fed high and low starch diets	27:104	Amylase addition increases starch ruminal digestion in first-lactation cows fed high and low starch diets.
24534508	10	45	theme	ruminal	1629:1635	arg1	digestion					1637:1645	ruminal digestion	1629:1645	ruminal digestion	1629:1645	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	10	46	from	changes	1508:1514	arg1	digestion					1637:1645	ruminal digestion	1629:1645	ruminal digestion	1629:1645	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	10	46	from	changes	1508:1514	arg1	production					1527:1536	production	1527:1536	production	1527:1536	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	10	46	from	changes	1508:1514	arg1	matter					1543:1548	dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility	1539:1623	matter	1543:1548	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	10	46	from	changes	1508:1514	arg1	intake					1519:1524	intake	1519:1524	intake	1519:1524	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	3	47	theme	exogenous	561:569	arg1	preparation					579:589	an exogenous amylase preparation	558:589	an exogenous amylase preparation	558:589	Corn silage-based diets had 20 or 30% starch, attained by changing the composition of concentrate, with or without addition of an exogenous amylase preparation.
24534508	10	48	theme	acid	1589:1592	arg1	fiber					1604:1608	acid detergent fiber	1589:1608	acid detergent fiber	1589:1608	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	8	49	from	concentration	1287:1299	arg1	rumen					1338:1342	the rumen	1334:1342	the rumen	1334:1342	Amylase supplement reduced the proportion of acetate and butyrate and increased that of propionate, particularly in the high-starch diet, where it tended to increase the concentration of total volatile fatty acids in the rumen.
24534508	9	50	theme	Other	1345:1349	arg1	activity					1381:1388	a higher amylase activity	1364:1388	a higher amylase activity in the solid-associated microbial community	1364:1432	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	9	50	theme	Other	1345:1349	arg1	effects					1351:1357	Other effects	1345:1357	Other effects	1345:1357	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	9	51	from	activity	1381:1388	arg1	community					1424:1432	the solid-associated microbial community	1393:1432	the solid-associated microbial community	1393:1432	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	7	52	theme	microbial	1083:1091	arg1	flow					1095:1098	microbial N flow	1083:1098	microbial N flow to the duodenum	1083:1114	The amylase supplement also increased the true ruminal digestibility of organic matter but did not affect microbial N flow to the duodenum.
24534508	2	53	theme	square	332:337	arg1	design					339:344	a 4×4 Latin square design	320:344	a 4×4 Latin square design	320:344	Rumen and total-tract nutrient digestibility were measured in a 4×4 Latin square design with 28-d periods using 4 first-lactation cows cannulated at the rumen and duodenum.
24534508	1	54	theme	high-starch	223:233	arg1	diets					235:239	low- and high-starch diets	214:239	low- and high-starch diets	214:239	The objective of this study was to evaluate the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle.
24534508	3	55	theme	Corn	431:434	arg1	diets					449:453	Corn silage-based diets	431:453	Corn silage-based diets	431:453	Corn silage-based diets had 20 or 30% starch, attained by changing the composition of concentrate, with or without addition of an exogenous amylase preparation.
24534508	9	56	theme	higher	1366:1371	arg1	activity					1381:1388	a higher amylase activity	1364:1388	a higher amylase activity in the solid-associated microbial community	1364:1432	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	9	56	theme	higher	1366:1371	arg1	effects					1351:1357	Other effects	1345:1357	Other effects	1345:1357	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	2	57	theme	4×4	322:324	arg1	design					339:344	a 4×4 Latin square design	320:344	a 4×4 Latin square design	320:344	Rumen and total-tract nutrient digestibility were measured in a 4×4 Latin square design with 28-d periods using 4 first-lactation cows cannulated at the rumen and duodenum.
24534508	10	58	theme	small	1658:1662	arg1	changes					1664:1670	small changes	1658:1670	small changes	1658:1670	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	4	59	theme	enzyme	607:612	arg1	Effects					592:598	Effects	592:598	Effects of the enzyme additive	592:621	Effects of the enzyme additive were observed on ruminal digestibility but not at the total-tract level.
24534508	10	60	theme	dry	1539:1541	arg1	matter					1543:1548	dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility	1539:1623	matter	1543:1548	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	7	61	theme	organic	1049:1055	arg1	matter					1057:1062	organic matter	1049:1062	organic matter	1049:1062	The amylase supplement also increased the true ruminal digestibility of organic matter but did not affect microbial N flow to the duodenum.
24534508	10	62	theme	amylolytic	1699:1708	arg1	bacteria					1710:1717	selected fibrolytic and amylolytic bacteria	1675:1717	selected fibrolytic and amylolytic bacteria	1675:1717	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	11	63	dep	intake	1878:1883	arg1	levels					1900:1905	levels	1900:1905	levels	1900:1905	We conclude that the exogenous amylase improved starch digestion in the rumen in first-lactation cows with moderate intake and production levels.
24534508	9	64	from	tendency	1440:1447	arg1	community					1424:1432	the solid-associated microbial community	1393:1432	the solid-associated microbial community	1393:1432	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	2	65	theme	nutrient	280:287	arg1	digestibility					289:301	total-tract nutrient digestibility	268:301	total-tract nutrient digestibility	268:301	Rumen and total-tract nutrient digestibility were measured in a 4×4 Latin square design with 28-d periods using 4 first-lactation cows cannulated at the rumen and duodenum.
24534508	9	66	theme	microbial	1414:1422	arg1	community					1424:1432	the solid-associated microbial community	1393:1432	the solid-associated microbial community	1393:1432	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	7	67	theme	true	1019:1022	arg1	digestibility					1032:1044	the true ruminal digestibility	1015:1044	the true ruminal digestibility of organic matter	1015:1062	The amylase supplement also increased the true ruminal digestibility of organic matter but did not affect microbial N flow to the duodenum.
24534508	1	68	theme	preparation	186:196	arg1	effect					155:160	the effect	151:160	the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle	151:255	The objective of this study was to evaluate the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle.
24534508	6	69	theme	starch	911:916	arg1	complete					929:936	complete	929:936	complete	929:936	This difference in ruminal starch digestion was compensated postruminally, so that the total-tract digestibility of starch was almost complete and did not differ between treatments.
24534508	6	69	theme	starch	911:916	arg1	digestibility					894:906	the total-tract digestibility	878:906	the total-tract digestibility of starch	878:916	This difference in ruminal starch digestion was compensated postruminally, so that the total-tract digestibility of starch was almost complete and did not differ between treatments.
24534508	11	70	from	rumen	1834:1838	arg1	cows					1859:1862	first-lactation cows	1843:1862	first-lactation cows with moderate intake and production levels	1843:1905	We conclude that the exogenous amylase improved starch digestion in the rumen in first-lactation cows with moderate intake and production levels.
24534508	7	71	theme	amylase	981:987	arg1	supplement					989:998	The amylase supplement	977:998	The amylase supplement	977:998	The amylase supplement also increased the true ruminal digestibility of organic matter but did not affect microbial N flow to the duodenum.
24534508	10	72	theme	selected	1675:1682	arg1	bacteria					1710:1717	selected fibrolytic and amylolytic bacteria	1675:1717	selected fibrolytic and amylolytic bacteria	1675:1717	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	1	73	from	digestion	201:209	arg1	cattle					250:255	dairy cattle	244:255	dairy cattle	244:255	The objective of this study was to evaluate the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle.
24534508	3	74	theme	amylase	571:577	arg1	preparation					579:589	an exogenous amylase preparation	558:589	an exogenous amylase preparation	558:589	Corn silage-based diets had 20 or 30% starch, attained by changing the composition of concentrate, with or without addition of an exogenous amylase preparation.
24534508	9	75	theme	lower	1453:1457	arg1	numbers					1459:1465	lower numbers	1453:1465	lower numbers of protozoa	1453:1477	Other effects were a higher amylase activity in the solid-associated microbial community and a tendency for lower numbers of protozoa.
24534508	10	76	theme	fibrolytic	1684:1693	arg1	bacteria					1710:1717	selected fibrolytic and amylolytic bacteria	1675:1717	selected fibrolytic and amylolytic bacteria	1675:1717	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	5	77	theme	amylase	770:776	arg1	supplementation					778:792	amylase supplementation	770:792	amylase supplementation	770:792	Ruminal digestibility of starch increased from 75% in control to 81% with amylase supplementation.
24534508	1	78	theme	study	129:133	arg1	objective					111:119	The objective	107:119	The objective of this study	107:133	The objective of this study was to evaluate the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle.
24534508	10	79	from	changes	1664:1670	arg1	digestion					1637:1645	ruminal digestion	1629:1645	ruminal digestion	1629:1645	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	10	79	from	changes	1664:1670	arg1	production					1527:1536	production	1527:1536	production	1527:1536	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	10	79	from	changes	1664:1670	arg1	matter					1543:1548	dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility	1539:1623	matter	1543:1548	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	10	79	from	changes	1664:1670	arg1	intake					1519:1524	intake	1519:1524	intake	1519:1524	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	11	80	theme	exogenous	1783:1791	arg1	amylase					1793:1799	the exogenous amylase	1779:1799	the exogenous amylase	1779:1799	We conclude that the exogenous amylase improved starch digestion in the rumen in first-lactation cows with moderate intake and production levels.
24534508	1	81	from	effect	155:160	arg1	digestion					201:209	digestion	201:209	digestion of low- and high-starch diets in dairy cattle	201:255	The objective of this study was to evaluate the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle.
24534508	0	82	theme	starch	93:98	arg1	diets					100:104	high and low starch diets	80:104	high and low starch diets	80:104	Amylase addition increases starch ruminal digestion in first-lactation cows fed high and low starch diets.
24534508	2	83	theme	total-tract	268:278	arg1	digestibility					289:301	total-tract nutrient digestibility	268:301	total-tract nutrient digestibility	268:301	Rumen and total-tract nutrient digestibility were measured in a 4×4 Latin square design with 28-d periods using 4 first-lactation cows cannulated at the rumen and duodenum.
24534508	5	84	theme	Ruminal	696:702	arg1	digestibility					704:716	Ruminal digestibility	696:716	Ruminal digestibility of starch	696:726	Ruminal digestibility of starch increased from 75% in control to 81% with amylase supplementation.
24534508	2	85	dep	rumen	411:415	arg1	the					407:409	the	407:409	the	407:409	Rumen and total-tract nutrient digestibility were measured in a 4×4 Latin square design with 28-d periods using 4 first-lactation cows cannulated at the rumen and duodenum.
24534508	0	86	from	digestion	42:50	arg1	cows					71:74	first-lactation cows	55:74	first-lactation cows fed high and low starch diets	55:104	Amylase addition increases starch ruminal digestion in first-lactation cows fed high and low starch diets.
24534508	8	87	theme	total	1304:1308	arg1	acids					1325:1329	total volatile fatty acids	1304:1329	total volatile fatty acids	1304:1329	Amylase supplement reduced the proportion of acetate and butyrate and increased that of propionate, particularly in the high-starch diet, where it tended to increase the concentration of total volatile fatty acids in the rumen.
24534508	3	88	dep	%	467:467	arg1	30					465:466	30	465:466	30	465:466	Corn silage-based diets had 20 or 30% starch, attained by changing the composition of concentrate, with or without addition of an exogenous amylase preparation.
24534508	3	88	dep	%	467:467	arg1	20					459:460	20	459:460	20	459:460	Corn silage-based diets had 20 or 30% starch, attained by changing the composition of concentrate, with or without addition of an exogenous amylase preparation.
24534508	8	89	theme	fatty	1319:1323	arg1	acids					1325:1329	total volatile fatty acids	1304:1329	total volatile fatty acids	1304:1329	Amylase supplement reduced the proportion of acetate and butyrate and increased that of propionate, particularly in the high-starch diet, where it tended to increase the concentration of total volatile fatty acids in the rumen.
24534508	10	90	theme	neutral	1561:1567	arg1	fiber					1579:1583	neutral detergent fiber	1561:1583	neutral detergent fiber	1561:1583	In contrast, we observed no changes in intake, production, dry matter and fiber (neutral detergent fiber and acid detergent fiber) digestibility, or ruminal digestion, and no or small changes on selected fibrolytic and amylolytic bacteria and on the microbial community in general.
24534508	0	91	theme	starch	27:32	arg1	digestion					42:50	starch ruminal digestion	27:50	starch ruminal digestion in first-lactation cows fed high and low starch diets	27:104	Amylase addition increases starch ruminal digestion in first-lactation cows fed high and low starch diets.
24534508	6	92	theme	ruminal	814:820	arg1	digestion					829:837	ruminal starch digestion	814:837	ruminal starch digestion	814:837	This difference in ruminal starch digestion was compensated postruminally, so that the total-tract digestibility of starch was almost complete and did not differ between treatments.
24534508	1	93	theme	low-	214:217	arg1	diets					235:239	low- and high-starch diets	214:239	low- and high-starch diets	214:239	The objective of this study was to evaluate the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle.
24534508	2	94	theme	first-lactation	372:386	arg1	cows					388:391	4 first-lactation cows	370:391	4 first-lactation cows cannulated at the rumen and duodenum	370:428	Rumen and total-tract nutrient digestibility were measured in a 4×4 Latin square design with 28-d periods using 4 first-lactation cows cannulated at the rumen and duodenum.
24534508	0	95	theme	first-lactation	55:69	arg1	cows					71:74	first-lactation cows	55:74	first-lactation cows fed high and low starch diets	55:104	Amylase addition increases starch ruminal digestion in first-lactation cows fed high and low starch diets.
24534508	11	96	with	cows	1859:1862	arg1	intake					1878:1883	moderate intake	1869:1883	moderate intake	1869:1883	We conclude that the exogenous amylase improved starch digestion in the rumen in first-lactation cows with moderate intake and production levels.
24534508	11	96	with	cows	1859:1862	arg1	production					1889:1898	production	1889:1898	production	1889:1898	We conclude that the exogenous amylase improved starch digestion in the rumen in first-lactation cows with moderate intake and production levels.
27112401	1	0	theme	high	403:406	arg1	efficiency					418:427	high catalytic efficiency	403:427	high catalytic efficiency of certain immobilized enzymes	403:458	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	6	1	theme	TG	1684:1685	arg1	determination					1667:1679	amperometric determination	1654:1679	amperometric determination of TG	1654:1685	The kinetic properties and analytes used for amperometric determination of TG are fully described for others to practice in a trained laboratory.
27112401	3	2	theme	than	996:999	arg1	diffusion					1001:1009	the same particle than diffusion	978:1009	the same particle than diffusion from one particle to the next	978:1039	These products can diffuse much more easily between enzymes on the same particle than diffusion from one particle to the next, in the reaction medium.
27112401	5	3	theme	oxide	1419:1423	arg1	acid					1521:1524	gold nanoparticles-polypyrrole-polyindole carboxylic acid	1468:1524	gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH)	1468:1541	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	3	theme	oxide	1419:1423	arg1	-chitosan					1447:1455	-chitosan	1447:1455	-chitosan (CHIT)	1447:1462	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	3	theme	oxide	1419:1423	arg1	ZnONPs					1440:1445	ZnONPs	1440:1445	ZnONPs	1440:1445	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	3	theme	oxide	1419:1423	arg1	nanoparticles					1425:1437	zinc oxide nanoparticles	1414:1437	zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively	1414:1606	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	3	4	theme	same	982:985	arg1	diffusion					1001:1009	the same particle than diffusion	978:1009	the same particle than diffusion from one particle to the next	978:1039	These products can diffuse much more easily between enzymes on the same particle than diffusion from one particle to the next, in the reaction medium.
27112401	2	5	theme	single	732:737	arg1	support					739:745	a single support	730:745	a single support	730:745	Co-immobilization of multiple enzymes required for a multistep reaction cascade on a single support is more efficient than separately immobilizing the corresponding enzymes and mixing them physically, since products of one enzyme could serve as reactants for another.
27112401	11	6	theme	number	2808:2813	arg1	presence					2794:2801	the presence	2790:2801	the presence of a number of serum substances, tested as potential interferences	2790:2868	The measurement of serum TG by co-immobilized enzymes was unaffected by the presence of a number of serum substances, tested as potential interferences.
27112401	5	7	theme	Pt	1573:1574	arg1	electrodes					1583:1592	Pt and Au electrodes	1573:1592	Pt and Au electrodes	1573:1592	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	1	8	theme	certain	432:438	arg1	enzymes					452:458	certain immobilized enzymes	432:458	certain immobilized enzymes	432:458	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	8	9	theme	enzymes	2073:2079	arg1	activities					2044:2053	The combined activities	2031:2053	The combined activities of co-immobilized enzymes	2031:2079	The combined activities of co-immobilized enzymes was tested amperometrically, and these composite nanobiocatalysts showed optimum activity within 4-5s, at pH 6.5-7.5 and 35°C, when polarized at a potential between 0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein.
27112401	1	10	theme	enzymes	452:458	arg1	kinetics					302:309	enzyme kinetics	295:309	enzyme kinetics	295:309	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	10	theme	enzymes	452:458	arg1	adsorption					535:544	strong adsorption	528:544	strong adsorption of the enzyme of interest by a number of different approaches	528:606	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	10	theme	enzymes	452:458	arg1	efficiency					418:427	high catalytic efficiency	403:427	high catalytic efficiency of certain immobilized enzymes	403:458	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	10	theme	enzymes	452:458	arg1	non-toxicity					461:472	non-toxicity	461:472	non-toxicity of some of the nanoparticle matrices	461:509	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	10	theme	enzymes	452:458	arg1	stability					517:525	high stability	512:525	high stability	512:525	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	10	theme	enzymes	452:458	arg1	transportability					629:644	faster electron transportability	613:644	faster electron transportability	613:644	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	7	11	theme	Cyclic	1755:1760	arg1	voltammetry					1762:1772	Cyclic voltammetry	1755:1772	Cyclic voltammetry	1755:1772	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	1	12	theme	electron	620:627	arg1	transportability					629:644	faster electron transportability	613:644	faster electron transportability	613:644	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	0	13	theme	Kinase	109:114	arg1	Immobilization					74:87	Covalent Immobilization	65:87	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.	0:172	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	12	14	theme	TG	2966:2967	arg1	analysis					2969:2976	TG analysis	2966:2976	TG analysis	2966:2976	Thus, co-immobilization of enzymes onto aggregates of NPs resulted in improved performance for TG analysis.
27112401	4	15	dep	faster	1143:1148	arg1	kinetics					1150:1157	kinetics	1150:1157	kinetics	1150:1157	Thus, co-immobilization of enzymes onto NP aggregates is expected to produce faster kinetics than their individual immobilizations on separate matrices.
27112401	8	16	theme	excellent	2290:2298	arg1	linearity					2300:2308	excellent linearity	2290:2308	excellent linearity	2290:2308	The combined activities of co-immobilized enzymes was tested amperometrically, and these composite nanobiocatalysts showed optimum activity within 4-5s, at pH 6.5-7.5 and 35°C, when polarized at a potential between 0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein.
27112401	11	17	theme	serum	2818:2822	arg1	substances					2824:2833	serum substances	2818:2833	serum substances	2818:2833	The measurement of serum TG by co-immobilized enzymes was unaffected by the presence of a number of serum substances, tested as potential interferences.
27112401	1	18	theme	surface	373:379	arg1	area					381:384	larger surface area	366:384	larger surface area to volume ratio	366:400	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	19	dep	considered	216:225	arg1	supports					234:241	supports	234:241	considered better supports for the immobilization of enzymes	216:275	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	10	20	theme	persons	2615:2621	arg1	sera					2576:2579	the sera	2572:2579	the sera of apparently healthy persons and persons suffering from hypertriglyceridemia, which is recognized as a leading cause for heart disease	2572:2715	Co-immobilized biocatalysts measured triglycerides in the sera of apparently healthy persons and persons suffering from hypertriglyceridemia, which is recognized as a leading cause for heart disease.
27112401	10	21	from	triglycerides	2555:2567	arg1	sera					2576:2579	the sera	2572:2579	the sera of apparently healthy persons and persons suffering from hypertriglyceridemia, which is recognized as a leading cause for heart disease	2572:2715	Co-immobilized biocatalysts measured triglycerides in the sera of apparently healthy persons and persons suffering from hypertriglyceridemia, which is recognized as a leading cause for heart disease.
27112401	0	22	theme	Oxidase	142:148	arg1	Immobilization					74:87	Covalent Immobilization	65:87	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.	0:172	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	7	23	from	bonding	2004:2010	arg1	AuPPy/Pin5COOH					2015:2028	AuPPy/Pin5COOH	2015:2028	AuPPy/Pin5COOH	2015:2028	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	5	24	theme	zinc	1414:1417	arg1	oxide					1419:1423	zinc oxide	1414:1423	zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively	1414:1606	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	10	25	theme	persons	2603:2609	arg1	sera					2576:2579	the sera	2572:2579	the sera of apparently healthy persons and persons suffering from hypertriglyceridemia, which is recognized as a leading cause for heart disease	2572:2715	Co-immobilized biocatalysts measured triglycerides in the sera of apparently healthy persons and persons suffering from hypertriglyceridemia, which is recognized as a leading cause for heart disease.
27112401	1	26	theme	interest	563:570	arg1	enzyme					553:558	the enzyme	549:558	the enzyme of interest	549:570	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	2	27	theme	multiple	668:675	arg1	enzymes					677:683	multiple enzymes	668:683	multiple enzymes required for a multistep reaction cascade	668:725	Co-immobilization of multiple enzymes required for a multistep reaction cascade on a single support is more efficient than separately immobilizing the corresponding enzymes and mixing them physically, since products of one enzyme could serve as reactants for another.
27112401	8	28	theme	lipid	2336:2340	arg1	50-700mg/dl					2317:2327	50-700mg/dl	2317:2327	50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein	2317:2385	The combined activities of co-immobilized enzymes was tested amperometrically, and these composite nanobiocatalysts showed optimum activity within 4-5s, at pH 6.5-7.5 and 35°C, when polarized at a potential between 0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein.
27112401	2	29	theme	reaction	710:717	arg1	cascade					719:725	a multistep reaction cascade	698:725	a multistep reaction cascade	698:725	Co-immobilization of multiple enzymes required for a multistep reaction cascade on a single support is more efficient than separately immobilizing the corresponding enzymes and mixing them physically, since products of one enzyme could serve as reactants for another.
27112401	5	30	theme	glycerol-3-phosphate	1248:1267	arg1	oxidase					1269:1275	glycerol-3-phosphate oxidase	1248:1275	glycerol-3-phosphate oxidase	1248:1275	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	1	31	theme	due	312:314	arg1	kinetics					302:309	enzyme kinetics	295:309	enzyme kinetics	295:309	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	12	32	theme	improved	2941:2948	arg1	performance					2950:2960	improved performance	2941:2960	improved performance for TG analysis	2941:2976	Thus, co-immobilization of enzymes onto aggregates of NPs resulted in improved performance for TG analysis.
27112401	6	33	theme	trained	1735:1741	arg1	laboratory					1743:1752	a trained laboratory	1733:1752	a trained laboratory	1733:1752	The kinetic properties and analytes used for amperometric determination of TG are fully described for others to practice in a trained laboratory.
27112401	0	34	theme	Metal	153:157	arg1	Nanoparticles					159:171	Metal Nanoparticles	153:171	Metal Nanoparticles	153:171	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	5	35	theme	glycerol	1227:1234	arg1	kinase					1236:1241	glycerol kinase	1227:1241	glycerol kinase	1227:1241	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	0	36	theme	Covalent	65:72	arg1	Immobilization					74:87	Covalent Immobilization	65:87	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.	0:172	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	4	37	theme	separate	1200:1207	arg1	matrices					1209:1216	separate matrices	1200:1216	separate matrices	1200:1216	Thus, co-immobilization of enzymes onto NP aggregates is expected to produce faster kinetics than their individual immobilizations on separate matrices.
27112401	1	38	theme	approaches	597:606	arg1	number					577:582	a number	575:582	a number of different approaches	575:606	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	8	39	theme	combined	2035:2042	arg1	activities					2044:2053	The combined activities	2031:2053	The combined activities of co-immobilized enzymes	2031:2079	The combined activities of co-immobilized enzymes was tested amperometrically, and these composite nanobiocatalysts showed optimum activity within 4-5s, at pH 6.5-7.5 and 35°C, when polarized at a potential between 0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein.
27112401	11	40	theme	co-immobilized	2749:2762	arg1	enzymes					2764:2770	co-immobilized enzymes	2749:2770	co-immobilized enzymes	2749:2770	The measurement of serum TG by co-immobilized enzymes was unaffected by the presence of a number of serum substances, tested as potential interferences.
27112401	6	41	theme	kinetic	1613:1619	arg1	properties					1621:1630	The kinetic properties	1609:1630	The kinetic properties	1609:1630	The kinetic properties and analytes used for amperometric determination of TG are fully described for others to practice in a trained laboratory.
27112401	1	42	theme	strong	528:533	arg1	adsorption					535:544	strong adsorption	528:544	strong adsorption of the enzyme of interest by a number of different approaches	528:606	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	0	43	theme	Rational	0:7	arg1	Design					9:14	Rational Design	0:14	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.	0:172	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	1	44	theme	enzymes	269:275	arg1	immobilization					251:264	the immobilization	247:264	the immobilization of enzymes	247:275	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	7	45	theme	glutaraldehyde	1955:1968	arg1	coupling					1970:1977	glutaraldehyde coupling	1955:1977	glutaraldehyde coupling	1955:1977	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	4	46	from	immobilizations	1181:1195	arg1	matrices					1209:1216	separate matrices	1200:1216	separate matrices	1200:1216	Thus, co-immobilization of enzymes onto NP aggregates is expected to produce faster kinetics than their individual immobilizations on separate matrices.
27112401	8	47	theme	optimum	2154:2160	arg1	activity					2162:2169	optimum activity	2154:2169	optimum activity	2154:2169	The combined activities of co-immobilized enzymes was tested amperometrically, and these composite nanobiocatalysts showed optimum activity within 4-5s, at pH 6.5-7.5 and 35°C, when polarized at a potential between 0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein.
27112401	7	48	theme	electron	1784:1791	arg1	microscopy					1793:1802	scanning electron microscopy	1775:1802	scanning electron microscopy	1775:1802	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	10	49	theme	leading	2685:2691	arg1	hypertriglyceridemia					2638:2657	hypertriglyceridemia	2638:2657	hypertriglyceridemia	2638:2657	Co-immobilized biocatalysts measured triglycerides in the sera of apparently healthy persons and persons suffering from hypertriglyceridemia, which is recognized as a leading cause for heart disease.
27112401	10	49	theme	leading	2685:2691	arg1	cause					2693:2697	a leading cause	2683:2697	a leading cause for heart disease	2683:2715	Co-immobilized biocatalysts measured triglycerides in the sera of apparently healthy persons and persons suffering from hypertriglyceridemia, which is recognized as a leading cause for heart disease.
27112401	8	50	theme	detection	2347:2355	arg1	limit					2357:2361	detection limit	2347:2361	detection limit of 20mg/dl	2347:2372	The combined activities of co-immobilized enzymes was tested amperometrically, and these composite nanobiocatalysts showed optimum activity within 4-5s, at pH 6.5-7.5 and 35°C, when polarized at a potential between 0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein.
27112401	10	51	theme	Co-immobilized	2518:2531	arg1	biocatalysts					2533:2544	Co-immobilized biocatalysts	2518:2544	Co-immobilized biocatalysts	2518:2544	Co-immobilized biocatalysts measured triglycerides in the sera of apparently healthy persons and persons suffering from hypertriglyceridemia, which is recognized as a leading cause for heart disease.
27112401	1	52	theme	enzyme	553:558	arg1	kinetics					302:309	enzyme kinetics	295:309	enzyme kinetics	295:309	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	52	theme	enzyme	553:558	arg1	adsorption					535:544	strong adsorption	528:544	strong adsorption of the enzyme of interest by a number of different approaches	528:606	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	52	theme	enzyme	553:558	arg1	efficiency					418:427	high catalytic efficiency	403:427	high catalytic efficiency of certain immobilized enzymes	403:458	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	52	theme	enzyme	553:558	arg1	non-toxicity					461:472	non-toxicity	461:472	non-toxicity of some of the nanoparticle matrices	461:509	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	52	theme	enzyme	553:558	arg1	stability					517:525	high stability	512:525	high stability	512:525	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	52	theme	enzyme	553:558	arg1	transportability					629:644	faster electron transportability	613:644	faster electron transportability	613:644	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	0	53	theme	Platforms	32:40	arg1	Design					9:14	Rational Design	0:14	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.	0:172	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	8	54	with	lipid	2336:2340	arg1	limit					2357:2361	detection limit	2347:2361	detection limit of 20mg/dl	2347:2372	The combined activities of co-immobilized enzymes was tested amperometrically, and these composite nanobiocatalysts showed optimum activity within 4-5s, at pH 6.5-7.5 and 35°C, when polarized at a potential between 0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein.
27112401	7	55	theme	electrochemical	1846:1860	arg1	spectra					1872:1878	electrochemical impedance spectra	1846:1878	electrochemical impedance spectra	1846:1878	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	5	56	from	co-immobilization	1352:1368	arg1	nanocomposites					1396:1409	nanocomposites	1396:1409	nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively	1396:1606	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	56	from	co-immobilization	1352:1368	arg1	nanoparticles					1425:1437	zinc oxide nanoparticles	1414:1437	zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively	1414:1606	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	11	57	theme	TG	2743:2744	arg1	measurement					2722:2732	The measurement	2718:2732	The measurement of serum TG by co-immobilized enzymes	2718:2770	The measurement of serum TG by co-immobilized enzymes was unaffected by the presence of a number of serum substances, tested as potential interferences.
27112401	5	58	theme	gold	1468:1471	arg1	AuPPy-Pin5COOH					1527:1540	AuPPy-Pin5COOH	1527:1540	AuPPy-Pin5COOH	1527:1540	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	58	theme	gold	1468:1471	arg1	acid					1521:1524	gold nanoparticles-polypyrrole-polyindole carboxylic acid	1468:1524	gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH)	1468:1541	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	58	theme	gold	1468:1471	arg1	nanoparticles					1425:1437	zinc oxide nanoparticles	1414:1437	zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively	1414:1606	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	7	59	theme	covalent	1896:1903	arg1	co-immobilization					1905:1921	their covalent co-immobilization	1890:1921	their covalent co-immobilization onto electrode surfaces	1890:1945	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	2	60	theme	corresponding	798:810	arg1	enzymes					812:818	the corresponding enzymes	794:818	the corresponding enzymes	794:818	Co-immobilization of multiple enzymes required for a multistep reaction cascade on a single support is more efficient than separately immobilizing the corresponding enzymes and mixing them physically, since products of one enzyme could serve as reactants for another.
27112401	5	61	theme	carboxylic	1510:1519	arg1	AuPPy-Pin5COOH					1527:1540	AuPPy-Pin5COOH	1527:1540	AuPPy-Pin5COOH	1527:1540	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	61	theme	carboxylic	1510:1519	arg1	acid					1521:1524	gold nanoparticles-polypyrrole-polyindole carboxylic acid	1468:1524	gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH)	1468:1541	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	61	theme	carboxylic	1510:1519	arg1	nanoparticles					1425:1437	zinc oxide nanoparticles	1414:1437	zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively	1414:1606	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	7	62	theme	infra-red	1823:1831	arg1	spectra					1833:1839	infra-red spectra	1823:1839	infra-red spectra	1823:1839	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	3	63	from	particle	1020:1027	arg1	diffusion					1001:1009	the same particle than diffusion	978:1009	the same particle than diffusion from one particle to the next	978:1039	These products can diffuse much more easily between enzymes on the same particle than diffusion from one particle to the next, in the reaction medium.
27112401	1	64	theme	nanoparticle	489:500	arg1	matrices					502:509	the nanoparticle matrices	485:509	the nanoparticle matrices	485:509	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	9	65	theme	co-immobilized	2405:2418	arg1	enzymes					2420:2426	co-immobilized enzymes	2405:2426	co-immobilized enzymes	2405:2426	The half life of co-immobilized enzymes was 7 months, when stored dry at 4°C which is very convenient for practical applications.
27112401	8	66	theme	composite	2120:2128	arg1	nanobiocatalysts					2130:2145	these composite nanobiocatalysts	2114:2145	these composite nanobiocatalysts	2114:2145	The combined activities of co-immobilized enzymes was tested amperometrically, and these composite nanobiocatalysts showed optimum activity within 4-5s, at pH 6.5-7.5 and 35°C, when polarized at a potential between 0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein.
27112401	3	67	theme	particle	987:994	arg1	diffusion					1001:1009	the same particle than diffusion	978:1009	the same particle than diffusion from one particle to the next	978:1039	These products can diffuse much more easily between enzymes on the same particle than diffusion from one particle to the next, in the reaction medium.
27112401	2	68	theme	enzyme	870:875	arg1	reactants					892:900	reactants	892:900	reactants for another	892:912	Co-immobilization of multiple enzymes required for a multistep reaction cascade on a single support is more efficient than separately immobilizing the corresponding enzymes and mixing them physically, since products of one enzyme could serve as reactants for another.
27112401	2	68	theme	enzyme	870:875	arg1	products					854:861	products	854:861	products of one enzyme	854:875	Co-immobilization of multiple enzymes required for a multistep reaction cascade on a single support is more efficient than separately immobilizing the corresponding enzymes and mixing them physically, since products of one enzyme could serve as reactants for another.
27112401	5	69	theme	enzymes	1385:1391	arg1	co-immobilization					1352:1368	the co-immobilization	1348:1368	the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively	1348:1606	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	1	70	theme	catalytic	408:416	arg1	efficiency					418:427	high catalytic efficiency	403:427	high catalytic efficiency of certain immobilized enzymes	403:458	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	7	71	theme	scanning	1775:1782	arg1	microscopy					1793:1802	scanning electron microscopy	1775:1802	scanning electron microscopy	1775:1802	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	4	72	theme	enzymes	1093:1099	arg1	co-immobilization					1072:1088	co-immobilization	1072:1088	co-immobilization of enzymes onto NP aggregates	1072:1118	Thus, co-immobilization of enzymes onto NP aggregates is expected to produce faster kinetics than their individual immobilizations on separate matrices.
27112401	5	73	theme	nanoparticles	1425:1437	arg1	nanocomposites					1396:1409	nanocomposites	1396:1409	nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively	1396:1606	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	73	theme	nanoparticles	1425:1437	arg1	nanoparticles					1425:1437	zinc oxide nanoparticles	1414:1437	zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively	1414:1606	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	4	74	theme	NP	1106:1107	arg1	aggregates					1109:1118	NP aggregates	1106:1118	NP aggregates	1106:1118	Thus, co-immobilization of enzymes onto NP aggregates is expected to produce faster kinetics than their individual immobilizations on separate matrices.
27112401	1	75	theme	immobilized	440:450	arg1	enzymes					452:458	certain immobilized enzymes	432:458	certain immobilized enzymes	432:458	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	7	76	dep	Fourier	1805:1811	arg1	transform					1813:1821	transform	1813:1821	transform infra-red spectra	1813:1839	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	1	77	theme	faster	613:618	arg1	transportability					629:644	faster electron transportability	613:644	faster electron transportability	613:644	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	11	78	theme	potential	2846:2854	arg1	interferences					2856:2868	potential interferences	2846:2868	potential interferences	2846:2868	The measurement of serum TG by co-immobilized enzymes was unaffected by the presence of a number of serum substances, tested as potential interferences.
27112401	1	79	theme	nanoparticles	192:204	arg1	aggregates					178:187	The aggregates	174:187	The aggregates of nanoparticles (NPs)	174:210	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	0	80	from	Immobilization	74:87	arg1	Nanoparticles					159:171	Metal Nanoparticles	153:171	Metal Nanoparticles	153:171	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	8	81	theme	Co-immobilized	2260:2273	arg1	enzymes					2275:2281	0.1 and 0.4V. Co-immobilized enzymes	2246:2281	0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein	2246:2385	The combined activities of co-immobilized enzymes was tested amperometrically, and these composite nanobiocatalysts showed optimum activity within 4-5s, at pH 6.5-7.5 and 35°C, when polarized at a potential between 0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein.
27112401	0	82	theme	Glycerol-3-Phosphate	121:140	arg1	Oxidase					142:148	Glycerol-3-Phosphate Oxidase	121:148	Glycerol-3-Phosphate Oxidase	121:148	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	1	83	theme	larger	366:371	arg1	area					381:384	larger surface area	366:384	larger surface area to volume ratio	366:400	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	7	84	from	CHIT-ZnONPs	1982:1992	arg1	AuPPy/Pin5COOH					2015:2028	AuPPy/Pin5COOH	2015:2028	AuPPy/Pin5COOH	2015:2028	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	5	85	from	analysis	1300:1307	arg1	reaction					1322:1329	a cascade reaction	1312:1329	a cascade reaction	1312:1329	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	11	86	theme	substances	2824:2833	arg1	number					2808:2813	a number	2806:2813	a number of serum substances, tested as potential interferences	2806:2868	The measurement of serum TG by co-immobilized enzymes was unaffected by the presence of a number of serum substances, tested as potential interferences.
27112401	2	87	theme	enzymes	677:683	arg1	Co-immobilization					647:663	Co-immobilization	647:663	Co-immobilization of multiple enzymes required for a multistep reaction cascade on a single support	647:745	Co-immobilization of multiple enzymes required for a multistep reaction cascade on a single support is more efficient than separately immobilizing the corresponding enzymes and mixing them physically, since products of one enzyme could serve as reactants for another.
27112401	1	88	theme	volume	389:394	arg1	ratio					396:400	volume ratio	389:400	volume ratio	389:400	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	9	89	theme	practical	2494:2502	arg1	applications					2504:2515	practical applications	2494:2515	practical applications	2494:2515	The half life of co-immobilized enzymes was 7 months, when stored dry at 4°C which is very convenient for practical applications.
27112401	5	90	dep	nanoparticles	1425:1437	arg1	acid					1521:1524	gold nanoparticles-polypyrrole-polyindole carboxylic acid	1468:1524	gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH)	1468:1541	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	90	dep	nanoparticles	1425:1437	arg1	CHIT					1458:1461	CHIT	1458:1461	CHIT	1458:1461	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	90	dep	nanoparticles	1425:1437	arg1	-chitosan					1447:1455	-chitosan	1447:1455	-chitosan (CHIT)	1447:1462	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	90	dep	nanoparticles	1425:1437	arg1	nanoparticles					1425:1437	zinc oxide nanoparticles	1414:1437	zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively	1414:1606	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	90	dep	nanoparticles	1425:1437	arg1	AuPPy-Pin5COOH					1527:1540	AuPPy-Pin5COOH	1527:1540	AuPPy-Pin5COOH	1527:1540	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	6	91	theme	amperometric	1654:1665	arg1	determination					1667:1679	amperometric determination	1654:1679	amperometric determination of TG	1654:1685	The kinetic properties and analytes used for amperometric determination of TG are fully described for others to practice in a trained laboratory.
27112401	10	92	theme	healthy	2595:2601	arg1	persons					2603:2609	apparently healthy persons	2584:2609	apparently healthy persons	2584:2609	Co-immobilized biocatalysts measured triglycerides in the sera of apparently healthy persons and persons suffering from hypertriglyceridemia, which is recognized as a leading cause for heart disease.
27112401	12	93	theme	NPs	2925:2927	arg1	aggregates					2911:2920	aggregates	2911:2920	aggregates of NPs	2911:2927	Thus, co-immobilization of enzymes onto aggregates of NPs resulted in improved performance for TG analysis.
27112401	2	94	theme	multistep	700:708	arg1	cascade					719:725	a multistep reaction cascade	698:725	a multistep reaction cascade	698:725	Co-immobilization of multiple enzymes required for a multistep reaction cascade on a single support is more efficient than separately immobilizing the corresponding enzymes and mixing them physically, since products of one enzyme could serve as reactants for another.
27112401	7	95	theme	amide	1998:2002	arg1	bonding					2004:2010	amide bonding	1998:2010	amide bonding	1998:2010	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	2	96	from	Co-immobilization	647:663	arg1	support					739:745	a single support	730:745	a single support	730:745	Co-immobilization of multiple enzymes required for a multistep reaction cascade on a single support is more efficient than separately immobilizing the corresponding enzymes and mixing them physically, since products of one enzyme could serve as reactants for another.
27112401	1	97	theme	different	587:595	arg1	approaches					597:606	different approaches	587:606	different approaches	587:606	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	0	98	theme	Lipase	92:97	arg1	Immobilization					74:87	Covalent Immobilization	65:87	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.	0:172	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	1	99	theme	unusual	325:331	arg1	area					381:384	larger surface area	366:384	larger surface area to volume ratio	366:400	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	99	theme	unusual	325:331	arg1	properties					347:356	their unusual but favorable properties	319:356	their unusual but favorable properties such as larger surface area to volume ratio	319:400	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	12	100	theme	enzymes	2898:2904	arg1	co-immobilization					2877:2893	co-immobilization	2877:2893	co-immobilization of enzymes onto aggregates of NPs	2877:2927	Thus, co-immobilization of enzymes onto aggregates of NPs resulted in improved performance for TG analysis.
27112401	1	101	theme	high	512:515	arg1	stability					517:525	high stability	512:525	high stability	512:525	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	0	102	theme	Glycerol	100:107	arg1	Kinase					109:114	Glycerol Kinase	100:114	Glycerol Kinase	100:114	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	1	103	theme	favorable	337:345	arg1	area					381:384	larger surface area	366:384	larger surface area to volume ratio	366:400	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	103	theme	favorable	337:345	arg1	properties					347:356	their unusual but favorable properties	319:356	their unusual but favorable properties such as larger surface area to volume ratio	319:400	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	3	104	theme	reaction	1049:1056	arg1	medium					1058:1063	the reaction medium	1045:1063	the reaction medium	1045:1063	These products can diffuse much more easily between enzymes on the same particle than diffusion from one particle to the next, in the reaction medium.
27112401	4	105	theme	individual	1170:1179	arg1	immobilizations					1181:1195	their individual immobilizations	1164:1195	their individual immobilizations on separate matrices	1164:1216	Thus, co-immobilization of enzymes onto NP aggregates is expected to produce faster kinetics than their individual immobilizations on separate matrices.
27112401	5	106	theme	cascade	1314:1320	arg1	reaction					1322:1329	a cascade reaction	1312:1329	a cascade reaction	1312:1329	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	8	107	theme	co-immobilized	2058:2071	arg1	enzymes					2073:2079	co-immobilized enzymes	2058:2079	co-immobilized enzymes	2058:2079	The combined activities of co-immobilized enzymes was tested amperometrically, and these composite nanobiocatalysts showed optimum activity within 4-5s, at pH 6.5-7.5 and 35°C, when polarized at a potential between 0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein.
27112401	3	108	from	enzymes	967:973	arg1	diffusion					1001:1009	the same particle than diffusion	978:1009	the same particle than diffusion from one particle to the next	978:1039	These products can diffuse much more easily between enzymes on the same particle than diffusion from one particle to the next, in the reaction medium.
27112401	0	109	theme	Nanoparticle	19:30	arg1	Platforms					32:40	Nanoparticle Platforms	19:40	Nanoparticle Platforms for "Cutting-the-Fat"	19:62	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	7	110	theme	electrode	1928:1936	arg1	surfaces					1938:1945	electrode surfaces	1928:1945	electrode surfaces	1928:1945	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	5	111	theme	lipid	1294:1298	arg1	analysis					1300:1307	lipid analysis	1294:1307	lipid analysis in a cascade reaction	1294:1329	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	11	112	theme	serum	2737:2741	arg1	TG					2743:2744	serum TG	2737:2744	serum TG	2737:2744	The measurement of serum TG by co-immobilized enzymes was unaffected by the presence of a number of serum substances, tested as potential interferences.
27112401	0	113	theme	Cutting-the-Fat	47:61	arg1	"					62:62	"Cutting-the-Fat"	46:62	"Cutting-the-Fat"	46:62	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	1	114	theme	enzyme	295:300	arg1	kinetics					302:309	enzyme kinetics	295:309	enzyme kinetics	295:309	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	7	115	theme	impedance	1862:1870	arg1	spectra					1872:1878	electrochemical impedance spectra	1846:1878	electrochemical impedance spectra	1846:1878	Cyclic voltammetry, scanning electron microscopy, Fourier transform infra-red spectra, and electrochemical impedance spectra confirmed their covalent co-immobilization onto electrode surfaces through glutaraldehyde coupling on CHIT-ZnONPs and amide bonding on AuPPy/Pin5COOH.
27112401	5	116	theme	nanoparticles-polypyrrole-polyindole	1473:1508	arg1	AuPPy-Pin5COOH					1527:1540	AuPPy-Pin5COOH	1527:1540	AuPPy-Pin5COOH	1527:1540	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	116	theme	nanoparticles-polypyrrole-polyindole	1473:1508	arg1	acid					1521:1524	gold nanoparticles-polypyrrole-polyindole carboxylic acid	1468:1524	gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH)	1468:1541	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	5	116	theme	nanoparticles-polypyrrole-polyindole	1473:1508	arg1	nanoparticles					1425:1437	zinc oxide nanoparticles	1414:1437	zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively	1414:1606	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	10	117	theme	heart	2703:2707	arg1	disease					2709:2715	heart disease	2703:2715	heart disease	2703:2715	Co-immobilized biocatalysts measured triglycerides in the sera of apparently healthy persons and persons suffering from hypertriglyceridemia, which is recognized as a leading cause for heart disease.
27112401	1	118	theme	some	477:480	arg1	kinetics					302:309	enzyme kinetics	295:309	enzyme kinetics	295:309	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	118	theme	some	477:480	arg1	adsorption					535:544	strong adsorption	528:544	strong adsorption of the enzyme of interest by a number of different approaches	528:606	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	118	theme	some	477:480	arg1	efficiency					418:427	high catalytic efficiency	403:427	high catalytic efficiency of certain immobilized enzymes	403:458	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	118	theme	some	477:480	arg1	non-toxicity					461:472	non-toxicity	461:472	non-toxicity of some of the nanoparticle matrices	461:509	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	118	theme	some	477:480	arg1	stability					517:525	high stability	512:525	high stability	512:525	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	1	118	theme	some	477:480	arg1	transportability					629:644	faster electron transportability	613:644	faster electron transportability	613:644	The aggregates of nanoparticles (NPs) are considered better supports for the immobilization of enzymes, as these promote enzyme kinetics, due to their unusual but favorable properties such as larger surface area to volume ratio, high catalytic efficiency of certain immobilized enzymes, non-toxicity of some of the nanoparticle matrices, high stability, strong adsorption of the enzyme of interest by a number of different approaches, and faster electron transportability.
27112401	9	119	theme	half	2392:2395	arg1	life					2397:2400	The half life	2388:2400	The half life of co-immobilized enzymes	2388:2426	The half life of co-immobilized enzymes was 7 months, when stored dry at 4°C which is very convenient for practical applications.
27112401	9	119	theme	half	2392:2395	arg1	months					2434:2439	7 months	2432:2439	7 months	2432:2439	The half life of co-immobilized enzymes was 7 months, when stored dry at 4°C which is very convenient for practical applications.
27112401	8	120	theme	20mg/dl	2366:2372	arg1	limit					2357:2361	detection limit	2347:2361	detection limit of 20mg/dl	2347:2372	The combined activities of co-immobilized enzymes was tested amperometrically, and these composite nanobiocatalysts showed optimum activity within 4-5s, at pH 6.5-7.5 and 35°C, when polarized at a potential between 0.1 and 0.4V. Co-immobilized enzymes showed excellent linearity within 50-700mg/dl of the lipid with detection limit of 20mg/dl for triolein.
27112401	0	121	dep	Design	9:14	arg1	Immobilization					74:87	Covalent Immobilization	65:87	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.	0:172	Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.
27112401	5	122	theme	Au	1580:1581	arg1	electrodes					1583:1592	Pt and Au electrodes	1573:1592	Pt and Au electrodes	1573:1592	Lipase, glycerol kinase, and glycerol-3-phosphate oxidase are required for lipid analysis in a cascade reaction, and we describe the co-immobilization of these three enzymes on nanocomposites of zinc oxide nanoparticles (ZnONPs)-chitosan (CHIT) and gold nanoparticles-polypyrrole-polyindole carboxylic acid (AuPPy-Pin5COOH) which are electrodeposited on Pt and Au electrodes, respectively.
27112401	9	123	theme	enzymes	2420:2426	arg1	life					2397:2400	The half life	2388:2400	The half life of co-immobilized enzymes	2388:2426	The half life of co-immobilized enzymes was 7 months, when stored dry at 4°C which is very convenient for practical applications.
27112401	9	123	theme	enzymes	2420:2426	arg1	months					2434:2439	7 months	2432:2439	7 months	2432:2439	The half life of co-immobilized enzymes was 7 months, when stored dry at 4°C which is very convenient for practical applications.
25817687	0	0	theme	JNK	89:91	arg1	pathways					93:100	ERK1/2 and JNK pathways	78:100	ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells	78:130	Polysaccharides from Smilax glabra inhibit the pro-inflammatory mediators via ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells.
25817687	4	1	theme	tumor	778:782	arg1	TNF-α					803:807	TNF-α	803:807	TNF-α	803:807	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	1	theme	tumor	778:782	arg1	factor-α					793:800	tumor necrosis factor-α	778:800	tumor necrosis factor-α (TNF-α)	778:808	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	2	theme	synthase	926:933	arg1	expression					889:898	the mRNA expression	880:898	the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6	880:956	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	2	theme	synthase	926:933	arg1	release					748:754	the release	744:754	the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells	744:866	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	1	3	theme	glabra	156:161	arg1	rhizomes					137:144	The rhizomes	133:144	The rhizomes of Smilax glabra	133:161	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	1	3	theme	glabra	156:161	arg1	food					186:189	food	186:189	food	186:189	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	1	3	theme	glabra	156:161	arg1	medicine					200:207	folk medicine	195:207	folk medicine in many countries for a long time	195:241	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	4	4	from	cells	862:866	arg1	expression					889:898	the mRNA expression	880:898	the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6	880:956	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	4	from	cells	862:866	arg1	release					748:754	the release	744:754	the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells	744:866	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	3	5	theme	average	450:456	arg1	weights					468:474	the number average molecular weights	439:474	the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa	439:513	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	4	6	theme	LPS-induced	840:850	arg1	cells					862:866	LPS-induced RAW 264.7 cells	840:866	LPS-induced RAW 264.7 cells	840:866	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	1	7	theme	long	233:236	arg1	time					238:241	a long time	231:241	a long time	231:241	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	4	8	theme	nitric	759:764	arg1	oxide					766:770	nitric oxide	759:770	nitric oxide (NO)	759:775	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	8	theme	nitric	759:764	arg1	NO					773:774	NO	773:774	NO	773:774	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	3	9	theme	×	565:565	arg1	kDa					572:574	1.31 × 10(5)kDa	560:574	1.31 × 10(5)kDa	560:574	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	10	dep	galactose	666:674	arg1	2.5:1					690:694	2.5:1	690:694	2.5:1	690:694	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	11	theme	×	484:484	arg1	kDa					491:493	1.72 × 10(2)kDa	479:493	1.72 × 10(2)kDa	479:493	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	5	12	theme	signal-regulated	1017:1032	arg1	ERK					1042:1044	ERK	1042:1044	ERK	1042:1044	Additionally, SGP-1 and SGP-2 repressed the extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK).
25817687	5	12	theme	signal-regulated	1017:1032	arg1	kinase					1034:1039	the extracellular signal-regulated kinase	999:1039	the extracellular signal-regulated kinase (ERK)	999:1045	Additionally, SGP-1 and SGP-2 repressed the extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK).
25817687	3	13	theme	mainly	621:626	arg1	compositions					643:654	their mainly monosaccharide compositions	615:654	their mainly monosaccharide compositions	615:654	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	14	theme	present	327:333	arg1	study					335:339	the present study	323:339	the present study	323:339	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	15	theme	kDa	572:574	arg1	compositions					643:654	their mainly monosaccharide compositions	615:654	their mainly monosaccharide compositions	615:654	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	15	theme	kDa	572:574	arg1	weights					549:555	the weight average molecular weights	520:555	the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa	520:594	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	15	theme	kDa	572:574	arg1	galactose					666:674	galactose	666:674	galactose	666:674	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	2	16	theme	little	253:258	arg1	research					260:267	little research	253:267	little research	253:267	However, little research has been reported on polysaccharides of S. glabra.
25817687	3	17	theme	monosaccharide	628:641	arg1	compositions					643:654	their mainly monosaccharide compositions	615:654	their mainly monosaccharide compositions	615:654	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	0	18	theme	RAW264.7	117:124	arg1	cells					126:130	LPS-induced RAW264.7 cells	105:130	LPS-induced RAW264.7 cells	105:130	Polysaccharides from Smilax glabra inhibit the pro-inflammatory mediators via ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells.
25817687	4	19	theme	mRNA	884:887	arg1	expression					889:898	the mRNA expression	880:898	the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6	880:956	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	0	20	theme	LPS-induced	105:115	arg1	cells					126:130	LPS-induced RAW264.7 cells	105:130	LPS-induced RAW264.7 cells	105:130	Polysaccharides from Smilax glabra inhibit the pro-inflammatory mediators via ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells.
25817687	4	21	theme	factor-α	793:800	arg1	expression					889:898	the mRNA expression	880:898	the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6	880:956	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	21	theme	factor-α	793:800	arg1	release					748:754	the release	744:754	the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells	744:866	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	3	22	attach	isolated	394:401	arg2	SGP-2					382:386	SGP-2	382:386	SGP-2	382:386	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	22	attach	isolated	394:401	arg2	SGP-1					372:376	SGP-1	372:376	SGP-1	372:376	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	22	attach	isolated	394:401	arg1	rhizomes					412:419	the rhizomes	408:419	the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa	408:513	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	22	attach	isolated	394:401	arg2	fractions					361:369	two polysaccharide fractions	342:369	two polysaccharide fractions	342:369	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	4	23	theme	inducible	903:911	arg1	synthase					926:933	inducible nitric oxide synthase	903:933	inducible nitric oxide synthase (iNOS)	903:940	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	23	theme	inducible	903:911	arg1	IL-6					953:956	IL-6	953:956	IL-6	953:956	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	23	theme	inducible	903:911	arg1	iNOS					936:939	iNOS	936:939	iNOS	936:939	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	23	theme	inducible	903:911	arg1	TNF-α					943:947	TNF-α	943:947	TNF-α	943:947	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	5	24	theme	extracellular	1003:1015	arg1	ERK					1042:1044	ERK	1042:1044	ERK	1042:1044	Additionally, SGP-1 and SGP-2 repressed the extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK).
25817687	5	24	theme	extracellular	1003:1015	arg1	kinase					1034:1039	the extracellular signal-regulated kinase	999:1039	the extracellular signal-regulated kinase (ERK)	999:1045	Additionally, SGP-1 and SGP-2 repressed the extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK).
25817687	3	25	theme	polysaccharide	346:359	arg1	SGP-2					382:386	SGP-2	382:386	SGP-2	382:386	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	25	theme	polysaccharide	346:359	arg1	SGP-1					372:376	SGP-1	372:376	SGP-1	372:376	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	25	theme	polysaccharide	346:359	arg1	fractions					361:369	two polysaccharide fractions	342:369	two polysaccharide fractions	342:369	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	26	theme	molecular	539:547	arg1	weights					549:555	the weight average molecular weights	520:555	the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa	520:594	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	26	theme	molecular	539:547	arg1	galactose					666:674	galactose	666:674	galactose	666:674	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	27	theme	kDa	491:493	arg1	weights					468:474	the number average molecular weights	439:474	the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa	439:513	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	5	28	theme	c-Jun	1051:1055	arg1	JNK					1078:1080	JNK	1078:1080	JNK	1078:1080	Additionally, SGP-1 and SGP-2 repressed the extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK).
25817687	5	28	theme	c-Jun	1051:1055	arg1	kinase					1070:1075	c-Jun NH2-terminal kinase	1051:1075	c-Jun NH2-terminal kinase (JNK)	1051:1081	Additionally, SGP-1 and SGP-2 repressed the extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK).
25817687	0	29	theme	Smilax	21:26	arg1	glabra					28:33	Smilax glabra	21:33	Smilax glabra	21:33	Polysaccharides from Smilax glabra inhibit the pro-inflammatory mediators via ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells.
25817687	6	30	theme	anti-inflammatory	1148:1164	arg1	ingredient					1173:1182	the anti-inflammatory active ingredient	1144:1182	the anti-inflammatory active ingredient for S. glabra	1144:1196	These findings strongly suggested polysaccharides were also the anti-inflammatory active ingredient for S. glabra, and the potential of SGP-1 and SGP-2 as the anti-inflammatory agents.
25817687	5	31	theme	NH2-terminal	1057:1068	arg1	JNK					1078:1080	JNK	1078:1080	JNK	1078:1080	Additionally, SGP-1 and SGP-2 repressed the extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK).
25817687	5	31	theme	NH2-terminal	1057:1068	arg1	kinase					1070:1075	c-Jun NH2-terminal kinase	1051:1075	c-Jun NH2-terminal kinase (JNK)	1051:1081	Additionally, SGP-1 and SGP-2 repressed the extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK).
25817687	3	32	theme	number	443:448	arg1	weights					468:474	the number average molecular weights	439:474	the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa	439:513	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	33	theme	×	585:585	arg1	kDa					592:594	1.18 × 10(5)kDa	580:594	1.18 × 10(5)kDa	580:594	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	34	with	rhizomes	412:419	arg1	weights					468:474	the number average molecular weights	439:474	the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa	439:513	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	35	theme	weight	524:529	arg1	weights					549:555	the weight average molecular weights	520:555	the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa	520:594	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	35	theme	weight	524:529	arg1	galactose					666:674	galactose	666:674	galactose	666:674	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	36	theme	×	504:504	arg1	kDa					511:513	1.31 × 10(2)kDa	499:513	1.31 × 10(2)kDa	499:513	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	6	37	theme	anti-inflammatory	1243:1259	arg1	agents					1261:1266	the anti-inflammatory agents	1239:1266	the anti-inflammatory agents	1239:1266	These findings strongly suggested polysaccharides were also the anti-inflammatory active ingredient for S. glabra, and the potential of SGP-1 and SGP-2 as the anti-inflammatory agents.
25817687	1	38	theme	folk	195:198	arg1	rhizomes					137:144	The rhizomes	133:144	The rhizomes of Smilax glabra	133:161	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	1	38	theme	folk	195:198	arg1	medicine					200:207	folk medicine	195:207	folk medicine in many countries for a long time	195:241	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	4	39	theme	necrosis	784:791	arg1	TNF-α					803:807	TNF-α	803:807	TNF-α	803:807	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	39	theme	necrosis	784:791	arg1	factor-α					793:800	tumor necrosis factor-α	778:800	tumor necrosis factor-α (TNF-α)	778:808	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	3	40	theme	kDa	592:594	arg1	compositions					643:654	their mainly monosaccharide compositions	615:654	their mainly monosaccharide compositions	615:654	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	40	theme	kDa	592:594	arg1	weights					549:555	the weight average molecular weights	520:555	the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa	520:594	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	40	theme	kDa	592:594	arg1	galactose					666:674	galactose	666:674	galactose	666:674	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	4	41	theme	oxide	766:770	arg1	expression					889:898	the mRNA expression	880:898	the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6	880:956	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	41	theme	oxide	766:770	arg1	release					748:754	the release	744:754	the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells	744:866	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	6	42	theme	active	1166:1171	arg1	ingredient					1173:1182	the anti-inflammatory active ingredient	1144:1182	the anti-inflammatory active ingredient for S. glabra	1144:1196	These findings strongly suggested polysaccharides were also the anti-inflammatory active ingredient for S. glabra, and the potential of SGP-1 and SGP-2 as the anti-inflammatory agents.
25817687	1	43	from	medicine	200:207	arg1	countries					217:225	many countries	212:225	many countries for a long time	212:241	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	2	44	theme	glabra	312:317	arg1	polysaccharides					290:304	polysaccharides	290:304	polysaccharides of S. glabra	290:317	However, little research has been reported on polysaccharides of S. glabra.
25817687	0	45	theme	pro-inflammatory	47:62	arg1	mediators					64:72	the pro-inflammatory mediators	43:72	the pro-inflammatory mediators	43:72	Polysaccharides from Smilax glabra inhibit the pro-inflammatory mediators via ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells.
25817687	1	46	from	food	186:189	arg1	countries					217:225	many countries	212:225	many countries for a long time	212:241	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	2	47	theme	S.	309:310	arg1	glabra					312:317	S. glabra	309:317	S. glabra	309:317	However, little research has been reported on polysaccharides of S. glabra.
25817687	4	48	theme	RAW	852:854	arg1	cells					862:866	LPS-induced RAW 264.7 cells	840:866	LPS-induced RAW 264.7 cells	840:866	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	3	49	theme	average	531:537	arg1	weights					549:555	the weight average molecular weights	520:555	the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa	520:594	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	49	theme	average	531:537	arg1	galactose					666:674	galactose	666:674	galactose	666:674	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	3	50	theme	kDa	511:513	arg1	weights					468:474	the number average molecular weights	439:474	the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa	439:513	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	4	51	theme	oxide	920:924	arg1	synthase					926:933	inducible nitric oxide synthase	903:933	inducible nitric oxide synthase (iNOS)	903:940	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	51	theme	oxide	920:924	arg1	IL-6					953:956	IL-6	953:956	IL-6	953:956	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	51	theme	oxide	920:924	arg1	iNOS					936:939	iNOS	936:939	iNOS	936:939	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	51	theme	oxide	920:924	arg1	TNF-α					943:947	TNF-α	943:947	TNF-α	943:947	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	3	52	theme	glabra	427:432	arg1	rhizomes					412:419	the rhizomes	408:419	the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa	408:513	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	6	53	theme	SGP-2	1230:1234	arg1	potential					1207:1215	the potential	1203:1215	the potential of SGP-1 and SGP-2 as the anti-inflammatory agents	1203:1266	These findings strongly suggested polysaccharides were also the anti-inflammatory active ingredient for S. glabra, and the potential of SGP-1 and SGP-2 as the anti-inflammatory agents.
25817687	6	53	theme	SGP-2	1230:1234	arg1	polysaccharides					1118:1132	polysaccharides	1118:1132	polysaccharides were also the anti-inflammatory active ingredient for S. glabra	1118:1196	These findings strongly suggested polysaccharides were also the anti-inflammatory active ingredient for S. glabra, and the potential of SGP-1 and SGP-2 as the anti-inflammatory agents.
25817687	1	54	theme	many	212:215	arg1	countries					217:225	many countries	212:225	many countries for a long time	212:241	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	0	55	theme	ERK1/2	78:83	arg1	pathways					93:100	ERK1/2 and JNK pathways	78:100	ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells	78:130	Polysaccharides from Smilax glabra inhibit the pro-inflammatory mediators via ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells.
25817687	0	56	from	pathways	93:100	arg1	cells					126:130	LPS-induced RAW264.7 cells	105:130	LPS-induced RAW264.7 cells	105:130	Polysaccharides from Smilax glabra inhibit the pro-inflammatory mediators via ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells.
25817687	1	57	used	used	173:176	arg2	medicine					200:207	folk medicine	195:207	folk medicine in many countries for a long time	195:241	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	1	57	used	used	173:176	arg2	rhizomes					137:144	The rhizomes	133:144	The rhizomes of Smilax glabra	133:161	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	1	57	used	used	173:176	arg2	food					186:189	food	186:189	food	186:189	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	4	58	theme	nitric	913:918	arg1	synthase					926:933	inducible nitric oxide synthase	903:933	inducible nitric oxide synthase (iNOS)	903:940	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	58	theme	nitric	913:918	arg1	IL-6					953:956	IL-6	953:956	IL-6	953:956	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	58	theme	nitric	913:918	arg1	iNOS					936:939	iNOS	936:939	iNOS	936:939	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	58	theme	nitric	913:918	arg1	TNF-α					943:947	TNF-α	943:947	TNF-α	943:947	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	3	59	theme	molecular	458:466	arg1	weights					468:474	the number average molecular weights	439:474	the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa	439:513	In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 × 10(2)kDa and 1.31 × 10(2)kDa, and the weight average molecular weights of 1.31 × 10(5)kDa and 1.18 × 10(5)kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1).
25817687	0	60	from	glabra	28:33	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Smilax glabra	0:33	Polysaccharides from Smilax glabra inhibit the pro-inflammatory mediators via ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells.
25817687	4	61	theme	interleukin-6	814:826	arg1	expression					889:898	the mRNA expression	880:898	the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6	880:956	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	4	61	theme	interleukin-6	814:826	arg1	release					748:754	the release	744:754	the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells	744:866	Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS), TNF-α and IL-6.
25817687	1	62	theme	Smilax	149:154	arg1	glabra					156:161	Smilax glabra	149:161	Smilax glabra	149:161	The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time.
25817687	6	63	theme	SGP-1	1220:1224	arg1	potential					1207:1215	the potential	1203:1215	the potential of SGP-1 and SGP-2 as the anti-inflammatory agents	1203:1266	These findings strongly suggested polysaccharides were also the anti-inflammatory active ingredient for S. glabra, and the potential of SGP-1 and SGP-2 as the anti-inflammatory agents.
25817687	6	63	theme	SGP-1	1220:1224	arg1	polysaccharides					1118:1132	polysaccharides	1118:1132	polysaccharides were also the anti-inflammatory active ingredient for S. glabra	1118:1196	These findings strongly suggested polysaccharides were also the anti-inflammatory active ingredient for S. glabra, and the potential of SGP-1 and SGP-2 as the anti-inflammatory agents.
27094334	8	0	theme	cell	1160:1163	arg1	structure					1170:1178	large-scale cell wall structure	1148:1178	large-scale cell wall structure	1148:1178	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	6	1	theme	Dectin-1	913:920	arg1	receptor					931:938	the Dectin-1 β-glucan receptor	909:938	the Dectin-1 β-glucan receptor	909:938	Histoplasma yeasts deficient for Eng1 show increased exposure of cell wall β-glucans, which results in enhanced binding to the Dectin-1 β-glucan receptor.
27094334	14	2	theme	wall	2690:2693	arg1	masking					2674:2680	masking	2674:2680	masking of cell wall	2674:2693	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	5	3	theme	Histoplasma	656:666	arg1	yeasts					668:673	Histoplasma yeasts	656:673	Histoplasma yeasts lacking Eng1 function	656:695	However, Histoplasma yeasts lacking Eng1 function have attenuated virulence in vivo, particularly during the cell-mediated immunity stage.
27094334	14	4	theme	cytokine	2512:2519	arg1	production					2521:2530	less proinflammatory cytokine production	2491:2530	less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo	2491:2594	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	10	5	theme	yeasts	1753:1758	arg1	ability					1742:1748	the ability	1738:1748	the ability of yeasts to escape detection by host phagocytes	1738:1797	Thus, Histoplasma Eng1 has evolved a specialized pathogenesis function to remove exposed β-glucans, thereby enhancing the ability of yeasts to escape detection by host phagocytes.
27094334	2	6	theme	antimicrobial	175:187	arg1	responses					196:204	antimicrobial immune responses	175:204	antimicrobial immune responses	175:204	To avoid antimicrobial immune responses, Histoplasma yeasts must minimize their detection by host receptors while simultaneously interacting with the phagocyte.
27094334	12	7	theme	Histoplasma	2023:2033	arg1	cells					2052:2056	Histoplasma pathogenic yeast cells	2023:2056	Histoplasma pathogenic yeast cells	2023:2056	In this study, we showed that Histoplasma pathogenic yeast cells, but not avirulent mycelia, secrete a β-glucanase, Eng1, which reduces recognition of fungal cell wall β-glucans.
27094334	6	8	theme	enhanced	889:896	arg1	binding					898:904	enhanced binding	889:904	enhanced binding to the Dectin-1 β-glucan receptor	889:938	Histoplasma yeasts deficient for Eng1 show increased exposure of cell wall β-glucans, which results in enhanced binding to the Dectin-1 β-glucan receptor.
27094334	7	9	theme	dendritic	1105:1113	arg1	cells					1115:1119	dendritic cells	1105:1119	dendritic cells	1105:1119	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	9	10	theme	β-glucan	1471:1478	arg1	exposure					1480:1487	β-glucan exposure	1471:1487	β-glucan exposure	1471:1487	In α-glucan-producing Histoplasma strains, Eng1 acts in concert with α-glucan to minimize β-glucan exposure: α-glucan provides a masking function by covering the β-glucan-rich cell wall, while Eng1 removes any remaining exposed β-glucans.
27094334	8	11	theme	yeast	1255:1259	arg1	wall					1266:1269	the yeast cell wall	1251:1269	the yeast cell wall	1251:1269	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	10	12	theme	Histoplasma	1626:1636	arg1	Eng1					1638:1641	Histoplasma Eng1	1626:1641	Histoplasma Eng1	1626:1641	Thus, Histoplasma Eng1 has evolved a specialized pathogenesis function to remove exposed β-glucans, thereby enhancing the ability of yeasts to escape detection by host phagocytes.
27094334	9	13	theme	exposed	1601:1607	arg1	β-glucans					1609:1617	any remaining exposed β-glucans	1587:1617	any remaining exposed β-glucans	1587:1617	In α-glucan-producing Histoplasma strains, Eng1 acts in concert with α-glucan to minimize β-glucan exposure: α-glucan provides a masking function by covering the β-glucan-rich cell wall, while Eng1 removes any remaining exposed β-glucans.
27094334	13	14	theme	Histoplasma	2326:2336	arg1	yeasts					2338:2343	Histoplasma yeasts	2326:2343	Histoplasma yeasts	2326:2343	We demonstrated that the Eng1 β-glucanase promotes Histoplasma virulence by reducing levels of surface-exposed β-glucans on yeast cells, thereby enabling Histoplasma yeasts to escape detection by the host β-glucan receptor, Dectin-1.
27094334	10	15	theme	pathogenesis	1669:1680	arg1	function					1682:1689	a specialized pathogenesis function	1655:1689	a specialized pathogenesis function	1655:1689	Thus, Histoplasma Eng1 has evolved a specialized pathogenesis function to remove exposed β-glucans, thereby enhancing the ability of yeasts to escape detection by host phagocytes.
27094334	4	16	theme	cell	630:633	arg1	separation					635:644	cell separation	630:644	cell separation	630:644	We show that Histoplasma Eng1 is a glucanase that hydrolyzes β-(1,3)-glycosyl linkages but is not required for Histoplasma growth in vitro or for cell separation.
27094334	2	17	theme	host	259:262	arg1	receptors					264:272	host receptors	259:272	host receptors	259:272	To avoid antimicrobial immune responses, Histoplasma yeasts must minimize their detection by host receptors while simultaneously interacting with the phagocyte.
27094334	9	18	theme	masking	1510:1516	arg1	function					1518:1525	a masking function	1508:1525	a masking function	1508:1525	In α-glucan-producing Histoplasma strains, Eng1 acts in concert with α-glucan to minimize β-glucan exposure: α-glucan provides a masking function by covering the β-glucan-rich cell wall, while Eng1 removes any remaining exposed β-glucans.
27094334	8	19	theme	exposed	1230:1236	arg1	β-glucans					1238:1246	exposed β-glucans	1230:1246	exposed β-glucans	1230:1246	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	8	20	theme	potential	1292:1300	arg1	recognition					1302:1312	potential recognition	1292:1312	potential recognition by Dectin-1	1292:1324	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	11	21	theme	intracellular	1855:1867	arg1	pathogen					1869:1876	an intracellular pathogen	1852:1876	an intracellular pathogen	1852:1876	IMPORTANCE The success of Histoplasma capsulatum as an intracellular pathogen results, in part, from an ability to minimize its detection by receptors on phagocytic cells of the immune system.
27094334	11	22	dep	IMPORTANCE	1800:1809	arg1	success					1815:1821	The success	1811:1821	IMPORTANCE The success of Histoplasma capsulatum as an intracellular pathogen	1800:1876	IMPORTANCE The success of Histoplasma capsulatum as an intracellular pathogen results, in part, from an ability to minimize its detection by receptors on phagocytic cells of the immune system.
27094334	11	23	theme	immune	1978:1983	arg1	system					1985:1990	the immune system	1974:1990	the immune system	1974:1990	IMPORTANCE The success of Histoplasma capsulatum as an intracellular pathogen results, in part, from an ability to minimize its detection by receptors on phagocytic cells of the immune system.
27094334	7	24	theme	factor	1018:1023	arg1	alpha					1025:1029	tumor necrosis factor alpha	1003:1029	tumor necrosis factor alpha (TNF-α)	1003:1037	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	7	24	theme	factor	1018:1023	arg1	TNF-α					1032:1036	TNF-α	1032:1036	TNF-α	1032:1036	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	3	25	theme	Histoplasma	338:348	arg1	cells					356:360	Pathogenic Histoplasma yeast cells	327:360	Pathogenic Histoplasma yeast cells	327:360	Pathogenic Histoplasma yeast cells, but not avirulent mycelial cells, secrete the Eng1 protein, which is a member of the glycosylhydrolase 81 (GH81) family.
27094334	5	26	theme	Eng1	683:686	arg1	function					688:695	Eng1 function	683:695	Eng1 function	683:695	However, Histoplasma yeasts lacking Eng1 function have attenuated virulence in vivo, particularly during the cell-mediated immunity stage.
27094334	7	27	theme	tumor	1003:1007	arg1	alpha					1025:1029	tumor necrosis factor alpha	1003:1029	tumor necrosis factor alpha (TNF-α)	1003:1037	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	7	27	theme	tumor	1003:1007	arg1	TNF-α					1032:1036	TNF-α	1032:1036	TNF-α	1032:1036	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	3	28	theme	avirulent	371:379	arg1	cells					390:394	avirulent mycelial cells	371:394	avirulent mycelial cells	371:394	Pathogenic Histoplasma yeast cells, but not avirulent mycelial cells, secrete the Eng1 protein, which is a member of the glycosylhydrolase 81 (GH81) family.
27094334	0	29	theme	β-Glucan	64:71	arg1	Exposure					73:80	β-Glucan Exposure	64:80	β-Glucan Exposure	64:80	The Eng1 β-Glucanase Enhances Histoplasma Virulence by Reducing β-Glucan Exposure.
27094334	9	30	theme	cell	1557:1560	arg1	wall					1562:1565	the β-glucan-rich cell wall	1539:1565	the β-glucan-rich cell wall	1539:1565	In α-glucan-producing Histoplasma strains, Eng1 acts in concert with α-glucan to minimize β-glucan exposure: α-glucan provides a masking function by covering the β-glucan-rich cell wall, while Eng1 removes any remaining exposed β-glucans.
27094334	14	31	theme	Histoplasma	2602:2612	arg1	yeasts					2614:2619	Histoplasma yeasts	2602:2619	Histoplasma yeasts	2602:2619	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	11	32	from	receptors	1941:1949	arg1	cells					1965:1969	phagocytic cells	1954:1969	phagocytic cells of the immune system	1954:1990	IMPORTANCE The success of Histoplasma capsulatum as an intracellular pathogen results, in part, from an ability to minimize its detection by receptors on phagocytic cells of the immune system.
27094334	8	33	theme	cytokine	1346:1353	arg1	production					1355:1364	proinflammatory cytokine production	1330:1364	proinflammatory cytokine production by phagocytes	1330:1378	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	5	34	theme	immunity	770:777	arg1	stage					779:783	the cell-mediated immunity stage	752:783	the cell-mediated immunity stage	752:783	However, Histoplasma yeasts lacking Eng1 function have attenuated virulence in vivo, particularly during the cell-mediated immunity stage.
27094334	7	35	theme	Eng1-deficient	963:976	arg1	yeasts					978:983	Eng1-deficient yeasts	963:983	Eng1-deficient yeasts	963:983	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	1	36	theme	UNLABELLED	83:92	arg1	capsulatum					126:135	The fungal pathogen Histoplasma capsulatum	94:135	UNLABELLED The fungal pathogen Histoplasma capsulatum	83:135	UNLABELLED The fungal pathogen Histoplasma capsulatum parasitizes host phagocytes.
27094334	1	37	theme	fungal	98:103	arg1	capsulatum					126:135	The fungal pathogen Histoplasma capsulatum	94:135	UNLABELLED The fungal pathogen Histoplasma capsulatum	83:135	UNLABELLED The fungal pathogen Histoplasma capsulatum parasitizes host phagocytes.
27094334	3	38	theme	Eng1	409:412	arg1	member					434:439	a member	432:439	a member of the glycosylhydrolase 81 (GH81) family	432:481	Pathogenic Histoplasma yeast cells, but not avirulent mycelial cells, secrete the Eng1 protein, which is a member of the glycosylhydrolase 81 (GH81) family.
27094334	3	38	theme	Eng1	409:412	arg1	protein					414:420	the Eng1 protein	405:420	the Eng1 protein	405:420	Pathogenic Histoplasma yeast cells, but not avirulent mycelial cells, secrete the Eng1 protein, which is a member of the glycosylhydrolase 81 (GH81) family.
27094334	7	39	from	macrophages	1089:1099	arg1	production					1073:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production	993:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells	993:1119	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	3	40	theme	family	476:481	arg1	member					434:439	a member	432:439	a member of the glycosylhydrolase 81 (GH81) family	432:481	Pathogenic Histoplasma yeast cells, but not avirulent mycelial cells, secrete the Eng1 protein, which is a member of the glycosylhydrolase 81 (GH81) family.
27094334	3	40	theme	family	476:481	arg1	protein					414:420	the Eng1 protein	405:420	the Eng1 protein	405:420	Pathogenic Histoplasma yeast cells, but not avirulent mycelial cells, secrete the Eng1 protein, which is a member of the glycosylhydrolase 81 (GH81) family.
27094334	14	41	theme	infection	2578:2586	arg1	production					2521:2530	less proinflammatory cytokine production	2491:2530	less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo	2491:2594	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	1	42	theme	Histoplasma	114:124	arg1	capsulatum					126:135	The fungal pathogen Histoplasma capsulatum	94:135	UNLABELLED The fungal pathogen Histoplasma capsulatum	83:135	UNLABELLED The fungal pathogen Histoplasma capsulatum parasitizes host phagocytes.
27094334	14	43	theme	β-glucans	2750:2758	arg1	α-glucan					2708:2715	α-glucan	2708:2715	α-glucan	2708:2715	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	14	43	theme	β-glucans	2750:2758	arg1	removal					2731:2737	enzymatic removal	2721:2737	enzymatic removal of exposed β-glucans by the Eng1 β-glucanase	2721:2782	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	4	44	theme	-glycosyl	552:560	arg1	linkages					562:569	β-(1,3)-glycosyl linkages	545:569	β-(1,3)-glycosyl linkages	545:569	We show that Histoplasma Eng1 is a glucanase that hydrolyzes β-(1,3)-glycosyl linkages but is not required for Histoplasma growth in vitro or for cell separation.
27094334	3	45	theme	GH81	470:473	arg1	family					476:481	the glycosylhydrolase 81 (GH81) family	444:481	the glycosylhydrolase 81 (GH81) family	444:481	Pathogenic Histoplasma yeast cells, but not avirulent mycelial cells, secrete the Eng1 protein, which is a member of the glycosylhydrolase 81 (GH81) family.
27094334	0	46	theme	Histoplasma	30:40	arg1	Virulence					42:50	Histoplasma Virulence	30:50	Histoplasma Virulence	30:50	The Eng1 β-Glucanase Enhances Histoplasma Virulence by Reducing β-Glucan Exposure.
27094334	13	47	theme	Eng1	2197:2200	arg1	β-glucanase					2202:2212	the Eng1 β-glucanase	2193:2212	the Eng1 β-glucanase	2193:2212	We demonstrated that the Eng1 β-glucanase promotes Histoplasma virulence by reducing levels of surface-exposed β-glucans on yeast cells, thereby enabling Histoplasma yeasts to escape detection by the host β-glucan receptor, Dectin-1.
27094334	6	48	theme	β-glucans	861:869	arg1	exposure					839:846	increased exposure	829:846	increased exposure	829:846	Histoplasma yeasts deficient for Eng1 show increased exposure of cell wall β-glucans, which results in enhanced binding to the Dectin-1 β-glucan receptor.
27094334	2	49	theme	immune	189:194	arg1	responses					196:204	antimicrobial immune responses	175:204	antimicrobial immune responses	175:204	To avoid antimicrobial immune responses, Histoplasma yeasts must minimize their detection by host receptors while simultaneously interacting with the phagocyte.
27094334	14	50	theme	yeasts	2461:2466	arg1	consequence					2411:2421	a consequence	2409:2421	a consequence	2409:2421	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	14	50	theme	yeasts	2461:2466	arg1	recognition					2434:2444	phagocyte recognition	2424:2444	phagocyte recognition of Histoplasma yeasts	2424:2466	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	9	51	theme	Histoplasma	1403:1413	arg1	strains					1415:1421	α-glucan-producing Histoplasma strains	1384:1421	α-glucan-producing Histoplasma strains	1384:1421	In α-glucan-producing Histoplasma strains, Eng1 acts in concert with α-glucan to minimize β-glucan exposure: α-glucan provides a masking function by covering the β-glucan-rich cell wall, while Eng1 removes any remaining exposed β-glucans.
27094334	6	52	theme	cell	851:854	arg1	β-glucans					861:869	cell wall β-glucans	851:869	cell wall β-glucans	851:869	Histoplasma yeasts deficient for Eng1 show increased exposure of cell wall β-glucans, which results in enhanced binding to the Dectin-1 β-glucan receptor.
27094334	14	53	theme	enzymatic	2721:2729	arg1	removal					2731:2737	enzymatic removal	2721:2737	enzymatic removal of exposed β-glucans by the Eng1 β-glucanase	2721:2782	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	12	54	theme	wall	2156:2159	arg1	β-glucans					2161:2169	fungal cell wall β-glucans	2144:2169	fungal cell wall β-glucans	2144:2169	In this study, we showed that Histoplasma pathogenic yeast cells, but not avirulent mycelia, secrete a β-glucanase, Eng1, which reduces recognition of fungal cell wall β-glucans.
27094334	13	55	theme	β-glucan	2377:2384	arg1	receptor					2386:2393	the host β-glucan receptor	2368:2393	the host β-glucan receptor	2368:2393	We demonstrated that the Eng1 β-glucanase promotes Histoplasma virulence by reducing levels of surface-exposed β-glucans on yeast cells, thereby enabling Histoplasma yeasts to escape detection by the host β-glucan receptor, Dectin-1.
27094334	13	55	theme	β-glucan	2377:2384	arg1	Dectin-1					2396:2403	Dectin-1	2396:2403	Dectin-1	2396:2403	We demonstrated that the Eng1 β-glucanase promotes Histoplasma virulence by reducing levels of surface-exposed β-glucans on yeast cells, thereby enabling Histoplasma yeasts to escape detection by the host β-glucan receptor, Dectin-1.
27094334	13	56	theme	surface-exposed	2267:2281	arg1	β-glucans					2283:2291	surface-exposed β-glucans	2267:2291	surface-exposed β-glucans	2267:2291	We demonstrated that the Eng1 β-glucanase promotes Histoplasma virulence by reducing levels of surface-exposed β-glucans on yeast cells, thereby enabling Histoplasma yeasts to escape detection by the host β-glucan receptor, Dectin-1.
27094334	10	57	theme	exposed	1701:1707	arg1	β-glucans					1709:1717	exposed β-glucans	1701:1717	exposed β-glucans	1701:1717	Thus, Histoplasma Eng1 has evolved a specialized pathogenesis function to remove exposed β-glucans, thereby enhancing the ability of yeasts to escape detection by host phagocytes.
27094334	14	58	theme	phagocyte	2424:2432	arg1	consequence					2411:2421	a consequence	2409:2421	a consequence	2409:2421	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	14	58	theme	phagocyte	2424:2432	arg1	recognition					2434:2444	phagocyte recognition	2424:2444	phagocyte recognition of Histoplasma yeasts	2424:2466	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	12	59	theme	pathogenic	2035:2044	arg1	cells					2052:2056	Histoplasma pathogenic yeast cells	2023:2056	Histoplasma pathogenic yeast cells	2023:2056	In this study, we showed that Histoplasma pathogenic yeast cells, but not avirulent mycelia, secrete a β-glucanase, Eng1, which reduces recognition of fungal cell wall β-glucans.
27094334	6	60	theme	β-glucan	922:929	arg1	receptor					931:938	the Dectin-1 β-glucan receptor	909:938	the Dectin-1 β-glucan receptor	909:938	Histoplasma yeasts deficient for Eng1 show increased exposure of cell wall β-glucans, which results in enhanced binding to the Dectin-1 β-glucan receptor.
27094334	14	61	theme	Histoplasma	2449:2459	arg1	yeasts					2461:2466	Histoplasma yeasts	2449:2466	Histoplasma yeasts	2449:2466	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	8	62	theme	large-scale	1148:1158	arg1	structure					1170:1178	large-scale cell wall structure	1148:1178	large-scale cell wall structure	1148:1178	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	14	63	theme	proinflammatory	2496:2510	arg1	production					2521:2530	less proinflammatory cytokine production	2491:2530	less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo	2491:2594	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	14	64	theme	cell	2685:2688	arg1	wall					2690:2693	cell wall	2685:2693	cell wall	2685:2693	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	8	65	theme	wall	1165:1168	arg1	structure					1170:1178	large-scale cell wall structure	1148:1178	large-scale cell wall structure	1148:1178	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	6	66	theme	deficient	805:813	arg1	yeasts					798:803	Histoplasma yeasts	786:803	Histoplasma yeasts deficient for Eng1	786:822	Histoplasma yeasts deficient for Eng1 show increased exposure of cell wall β-glucans, which results in enhanced binding to the Dectin-1 β-glucan receptor.
27094334	10	67	theme	host	1783:1786	arg1	phagocytes					1788:1797	host phagocytes	1783:1797	host phagocytes	1783:1797	Thus, Histoplasma Eng1 has evolved a specialized pathogenesis function to remove exposed β-glucans, thereby enhancing the ability of yeasts to escape detection by host phagocytes.
27094334	12	68	theme	fungal	2144:2149	arg1	β-glucans					2161:2169	fungal cell wall β-glucans	2144:2169	fungal cell wall β-glucans	2144:2169	In this study, we showed that Histoplasma pathogenic yeast cells, but not avirulent mycelia, secrete a β-glucanase, Eng1, which reduces recognition of fungal cell wall β-glucans.
27094334	8	69	theme	β-glucans	1238:1246	arg1	levels					1220:1225	levels	1220:1225	levels of exposed β-glucans	1220:1246	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	8	70	dep	not	1128:1130	arg1	responsible					1132:1142	responsible	1132:1142	responsible	1132:1142	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	6	71	theme	Histoplasma	786:796	arg1	yeasts					798:803	Histoplasma yeasts	786:803	Histoplasma yeasts deficient for Eng1	786:822	Histoplasma yeasts deficient for Eng1 show increased exposure of cell wall β-glucans, which results in enhanced binding to the Dectin-1 β-glucan receptor.
27094334	7	72	theme	cytokine	1064:1071	arg1	production					1073:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production	993:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells	993:1119	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	8	73	theme	secreted	1198:1205	arg1	Eng1					1207:1210	the secreted Eng1	1194:1210	the secreted Eng1	1194:1210	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	7	74	with	Consistent	941:950	arg1	this					957:960	this	957:960	this	957:960	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	3	75	theme	Pathogenic	327:336	arg1	cells					356:360	Pathogenic Histoplasma yeast cells	327:360	Pathogenic Histoplasma yeast cells	327:360	Pathogenic Histoplasma yeast cells, but not avirulent mycelial cells, secrete the Eng1 protein, which is a member of the glycosylhydrolase 81 (GH81) family.
27094334	12	76	theme	avirulent	2067:2075	arg1	mycelia					2077:2083	avirulent mycelia	2067:2083	avirulent mycelia	2067:2083	In this study, we showed that Histoplasma pathogenic yeast cells, but not avirulent mycelia, secrete a β-glucanase, Eng1, which reduces recognition of fungal cell wall β-glucans.
27094334	8	77	theme	cell	1261:1264	arg1	wall					1266:1269	the yeast cell wall	1251:1269	the yeast cell wall	1251:1269	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	10	78	theme	specialized	1657:1667	arg1	function					1682:1689	a specialized pathogenesis function	1655:1689	a specialized pathogenesis function	1655:1689	Thus, Histoplasma Eng1 has evolved a specialized pathogenesis function to remove exposed β-glucans, thereby enhancing the ability of yeasts to escape detection by host phagocytes.
27094334	11	79	theme	capsulatum	1838:1847	arg1	success					1815:1821	The success	1811:1821	IMPORTANCE The success of Histoplasma capsulatum as an intracellular pathogen	1800:1876	IMPORTANCE The success of Histoplasma capsulatum as an intracellular pathogen results, in part, from an ability to minimize its detection by receptors on phagocytic cells of the immune system.
27094334	5	80	theme	attenuated	702:711	arg1	virulence					713:721	attenuated virulence	702:721	attenuated virulence	702:721	However, Histoplasma yeasts lacking Eng1 function have attenuated virulence in vivo, particularly during the cell-mediated immunity stage.
27094334	11	81	theme	system	1985:1990	arg1	cells					1965:1969	phagocytic cells	1954:1969	phagocytic cells of the immune system	1954:1990	IMPORTANCE The success of Histoplasma capsulatum as an intracellular pathogen results, in part, from an ability to minimize its detection by receptors on phagocytic cells of the immune system.
27094334	14	82	theme	phagocyte	2653:2661	arg1	detection					2663:2671	phagocyte detection	2653:2671	phagocyte detection	2653:2671	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	7	83	theme	interleukin-6	1043:1055	arg1	production					1073:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production	993:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells	993:1119	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	13	84	theme	yeast	2296:2300	arg1	cells					2302:2306	yeast cells	2296:2306	yeast cells	2296:2306	We demonstrated that the Eng1 β-glucanase promotes Histoplasma virulence by reducing levels of surface-exposed β-glucans on yeast cells, thereby enabling Histoplasma yeasts to escape detection by the host β-glucan receptor, Dectin-1.
27094334	7	85	theme	alpha	1025:1029	arg1	production					1073:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production	993:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells	993:1119	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	1	86	theme	host	149:152	arg1	phagocytes					154:163	host phagocytes	149:163	host phagocytes	149:163	UNLABELLED The fungal pathogen Histoplasma capsulatum parasitizes host phagocytes.
27094334	3	87	theme	yeast	350:354	arg1	cells					356:360	Pathogenic Histoplasma yeast cells	327:360	Pathogenic Histoplasma yeast cells	327:360	Pathogenic Histoplasma yeast cells, but not avirulent mycelial cells, secrete the Eng1 protein, which is a member of the glycosylhydrolase 81 (GH81) family.
27094334	7	88	theme	necrosis	1009:1016	arg1	alpha					1025:1029	tumor necrosis factor alpha	1003:1029	tumor necrosis factor alpha (TNF-α)	1003:1037	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	7	88	theme	necrosis	1009:1016	arg1	TNF-α					1032:1036	TNF-α	1032:1036	TNF-α	1032:1036	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	9	89	theme	β-glucan-rich	1543:1555	arg1	wall					1562:1565	the β-glucan-rich cell wall	1539:1565	the β-glucan-rich cell wall	1539:1565	In α-glucan-producing Histoplasma strains, Eng1 acts in concert with α-glucan to minimize β-glucan exposure: α-glucan provides a masking function by covering the β-glucan-rich cell wall, while Eng1 removes any remaining exposed β-glucans.
27094334	3	90	theme	mycelial	381:388	arg1	cells					390:394	avirulent mycelial cells	371:394	avirulent mycelial cells	371:394	Pathogenic Histoplasma yeast cells, but not avirulent mycelial cells, secrete the Eng1 protein, which is a member of the glycosylhydrolase 81 (GH81) family.
27094334	5	91	theme	cell-mediated	756:768	arg1	immunity					770:777	the cell-mediated immunity	752:777	the cell-mediated immunity stage	752:783	However, Histoplasma yeasts lacking Eng1 function have attenuated virulence in vivo, particularly during the cell-mediated immunity stage.
27094334	7	92	theme	increased	993:1001	arg1	production					1073:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production	993:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells	993:1119	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	13	93	theme	β-glucans	2283:2291	arg1	levels					2257:2262	levels	2257:2262	levels of surface-exposed β-glucans	2257:2291	We demonstrated that the Eng1 β-glucanase promotes Histoplasma virulence by reducing levels of surface-exposed β-glucans on yeast cells, thereby enabling Histoplasma yeasts to escape detection by the host β-glucan receptor, Dectin-1.
27094334	8	94	theme	proinflammatory	1330:1344	arg1	production					1355:1364	proinflammatory cytokine production	1330:1364	proinflammatory cytokine production by phagocytes	1330:1378	While not responsible for large-scale cell wall structure and function, the secreted Eng1 reduces levels of exposed β-glucans at the yeast cell wall, thereby diminishing potential recognition by Dectin-1 and proinflammatory cytokine production by phagocytes.
27094334	4	95	theme	Histoplasma	497:507	arg1	Eng1					509:512	Histoplasma Eng1	497:512	Histoplasma Eng1	497:512	We show that Histoplasma Eng1 is a glucanase that hydrolyzes β-(1,3)-glycosyl linkages but is not required for Histoplasma growth in vitro or for cell separation.
27094334	4	95	theme	Histoplasma	497:507	arg1	glucanase					519:527	a glucanase	517:527	a glucanase that hydrolyzes β-(1,3)-glycosyl linkages but is not required for Histoplasma growth in vitro or for cell separation	517:644	We show that Histoplasma Eng1 is a glucanase that hydrolyzes β-(1,3)-glycosyl linkages but is not required for Histoplasma growth in vitro or for cell separation.
27094334	0	96	theme	Eng1	4:7	arg1	β-Glucanase					9:19	The Eng1 β-Glucanase	0:19	The Eng1 β-Glucanase	0:19	The Eng1 β-Glucanase Enhances Histoplasma Virulence by Reducing β-Glucan Exposure.
27094334	9	97	theme	remaining	1591:1599	arg1	β-glucans					1609:1617	any remaining exposed β-glucans	1587:1617	any remaining exposed β-glucans	1587:1617	In α-glucan-producing Histoplasma strains, Eng1 acts in concert with α-glucan to minimize β-glucan exposure: α-glucan provides a masking function by covering the β-glucan-rich cell wall, while Eng1 removes any remaining exposed β-glucans.
27094334	14	98	theme	Eng1	2767:2770	arg1	β-glucanase					2772:2782	the Eng1 β-glucanase	2763:2782	the Eng1 β-glucanase	2763:2782	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	3	99	theme	glycosylhydrolase	448:464	arg1	family					476:481	the glycosylhydrolase 81 (GH81) family	444:481	the glycosylhydrolase 81 (GH81) family	444:481	Pathogenic Histoplasma yeast cells, but not avirulent mycelial cells, secrete the Eng1 protein, which is a member of the glycosylhydrolase 81 (GH81) family.
27094334	1	100	theme	pathogen	105:112	arg1	capsulatum					126:135	The fungal pathogen Histoplasma capsulatum	94:135	UNLABELLED The fungal pathogen Histoplasma capsulatum	83:135	UNLABELLED The fungal pathogen Histoplasma capsulatum parasitizes host phagocytes.
27094334	5	101	contain	have	697:700	arg2	virulence					713:721	attenuated virulence	702:721	attenuated virulence	702:721	However, Histoplasma yeasts lacking Eng1 function have attenuated virulence in vivo, particularly during the cell-mediated immunity stage.
27094334	5	101	contain	have	697:700	arg1	yeasts					668:673	Histoplasma yeasts	656:673	Histoplasma yeasts lacking Eng1 function	656:695	However, Histoplasma yeasts lacking Eng1 function have attenuated virulence in vivo, particularly during the cell-mediated immunity stage.
27094334	2	102	theme	Histoplasma	207:217	arg1	yeasts					219:224	Histoplasma yeasts	207:224	Histoplasma yeasts	207:224	To avoid antimicrobial immune responses, Histoplasma yeasts must minimize their detection by host receptors while simultaneously interacting with the phagocyte.
27094334	14	103	theme	Histoplasma	2566:2576	arg1	infection					2578:2586	Histoplasma infection	2566:2586	Histoplasma infection in vivo	2566:2594	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	14	104	theme	exposed	2742:2748	arg1	β-glucans					2750:2758	exposed β-glucans	2742:2758	exposed β-glucans	2742:2758	As a consequence, phagocyte recognition of Histoplasma yeasts is reduced, leading to less proinflammatory cytokine production by phagocytes and less control of Histoplasma infection in vivo Thus, Histoplasma yeasts express two mechanisms to avoid phagocyte detection: masking of cell wall β-glucans by α-glucan and enzymatic removal of exposed β-glucans by the Eng1 β-glucanase.
27094334	7	105	from	cells	1115:1119	arg1	production					1073:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production	993:1082	increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells	993:1119	Consistent with this, Eng1-deficient yeasts trigger increased tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) cytokine production from macrophages and dendritic cells.
27094334	11	106	theme	phagocytic	1954:1963	arg1	cells					1965:1969	phagocytic cells	1954:1969	phagocytic cells of the immune system	1954:1990	IMPORTANCE The success of Histoplasma capsulatum as an intracellular pathogen results, in part, from an ability to minimize its detection by receptors on phagocytic cells of the immune system.
27094334	9	107	theme	α-glucan-producing	1384:1401	arg1	strains					1415:1421	α-glucan-producing Histoplasma strains	1384:1421	α-glucan-producing Histoplasma strains	1384:1421	In α-glucan-producing Histoplasma strains, Eng1 acts in concert with α-glucan to minimize β-glucan exposure: α-glucan provides a masking function by covering the β-glucan-rich cell wall, while Eng1 removes any remaining exposed β-glucans.
27094334	6	108	theme	wall	856:859	arg1	β-glucans					861:869	cell wall β-glucans	851:869	cell wall β-glucans	851:869	Histoplasma yeasts deficient for Eng1 show increased exposure of cell wall β-glucans, which results in enhanced binding to the Dectin-1 β-glucan receptor.
27094334	9	109	with	concert	1437:1443	arg1	α-glucan					1450:1457	α-glucan	1450:1457	α-glucan	1450:1457	In α-glucan-producing Histoplasma strains, Eng1 acts in concert with α-glucan to minimize β-glucan exposure: α-glucan provides a masking function by covering the β-glucan-rich cell wall, while Eng1 removes any remaining exposed β-glucans.
27094334	13	110	theme	Histoplasma	2223:2233	arg1	virulence					2235:2243	Histoplasma virulence	2223:2243	Histoplasma virulence	2223:2243	We demonstrated that the Eng1 β-glucanase promotes Histoplasma virulence by reducing levels of surface-exposed β-glucans on yeast cells, thereby enabling Histoplasma yeasts to escape detection by the host β-glucan receptor, Dectin-1.
27094334	13	111	theme	host	2372:2375	arg1	receptor					2386:2393	the host β-glucan receptor	2368:2393	the host β-glucan receptor	2368:2393	We demonstrated that the Eng1 β-glucanase promotes Histoplasma virulence by reducing levels of surface-exposed β-glucans on yeast cells, thereby enabling Histoplasma yeasts to escape detection by the host β-glucan receptor, Dectin-1.
27094334	13	111	theme	host	2372:2375	arg1	Dectin-1					2396:2403	Dectin-1	2396:2403	Dectin-1	2396:2403	We demonstrated that the Eng1 β-glucanase promotes Histoplasma virulence by reducing levels of surface-exposed β-glucans on yeast cells, thereby enabling Histoplasma yeasts to escape detection by the host β-glucan receptor, Dectin-1.
27094334	4	112	theme	Histoplasma	595:605	arg1	growth					607:612	Histoplasma growth	595:612	Histoplasma growth	595:612	We show that Histoplasma Eng1 is a glucanase that hydrolyzes β-(1,3)-glycosyl linkages but is not required for Histoplasma growth in vitro or for cell separation.
27094334	12	113	theme	cell	2151:2154	arg1	β-glucans					2161:2169	fungal cell wall β-glucans	2144:2169	fungal cell wall β-glucans	2144:2169	In this study, we showed that Histoplasma pathogenic yeast cells, but not avirulent mycelia, secrete a β-glucanase, Eng1, which reduces recognition of fungal cell wall β-glucans.
27094334	12	114	theme	yeast	2046:2050	arg1	cells					2052:2056	Histoplasma pathogenic yeast cells	2023:2056	Histoplasma pathogenic yeast cells	2023:2056	In this study, we showed that Histoplasma pathogenic yeast cells, but not avirulent mycelia, secrete a β-glucanase, Eng1, which reduces recognition of fungal cell wall β-glucans.
27094334	6	115	theme	increased	829:837	arg1	exposure					839:846	increased exposure	829:846	increased exposure	829:846	Histoplasma yeasts deficient for Eng1 show increased exposure of cell wall β-glucans, which results in enhanced binding to the Dectin-1 β-glucan receptor.
27094334	12	116	theme	β-glucans	2161:2169	arg1	recognition					2129:2139	recognition	2129:2139	recognition of fungal cell wall β-glucans	2129:2169	In this study, we showed that Histoplasma pathogenic yeast cells, but not avirulent mycelia, secrete a β-glucanase, Eng1, which reduces recognition of fungal cell wall β-glucans.
26020907	8	0	theme	d	946:946	arg1	length					927:932	a gestation length	915:932	a gestation length (GL) of 113 d	915:946	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	8	0	theme	d	946:946	arg1	GL					935:936	GL	935:936	GL	935:936	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	9	1	theme	piglets	1110:1116	arg1	weight					1091:1096	the litter birth weight	1074:1096	the litter birth weight of suckling piglets (LW)	1074:1121	An interaction was found between the litter birth weight of suckling piglets (LW) and GL ( = 0.03).
26020907	9	1	theme	piglets	1110:1116	arg1	GL					1127:1128	GL	1127:1128	GL ( = 0.03)	1127:1138	An interaction was found between the litter birth weight of suckling piglets (LW) and GL ( = 0.03).
26020907	9	1	theme	piglets	1110:1116	arg1	LW					1119:1120	LW	1119:1120	LW	1119:1120	An interaction was found between the litter birth weight of suckling piglets (LW) and GL ( = 0.03).
26020907	10	2	theme	higher	1194:1199	arg1	LW					1201:1202	higher LW	1194:1202	higher LW ( = 0.009)	1194:1213	In sows with a GL of 114 to 115 d, CY increased with higher LW ( = 0.009).
26020907	2	3	theme	present	223:229	arg1	study					245:249	The present observational study	219:249	The present observational study	219:249	The present observational study aimed to investigate factors influencing CY and CC in 10 commercial herds.
26020907	12	4	theme	Danbred	1469:1475	arg1	sows					1500:1503	PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows	1403:1503	PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows	1403:1503	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	2	5	from	CY	292:293	arg1	herds					319:323	10 commercial herds	305:323	10 commercial herds	305:323	The present observational study aimed to investigate factors influencing CY and CC in 10 commercial herds.
26020907	2	6	from	CC	299:300	arg1	herds					319:323	10 commercial herds	305:323	10 commercial herds	305:323	The present observational study aimed to investigate factors influencing CY and CC in 10 commercial herds.
26020907	14	7	theme	multivariable	1755:1767	arg1	model					1769:1773	the multivariable model	1751:1773	the multivariable model	1751:1773	The percentages of colostral protein and lactose were not significantly associated with any factor in the multivariable model.
26020907	8	8	theme	d	1014:1014	arg1	GL					997:998	a GL	995:998	a GL of 114 to 115 d (3,342 ± 107 g; = 0.04)	995:1038	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	7	9	theme	mixed	843:847	arg1	model					860:864	a linear mixed regression model	834:864	a linear mixed regression model with herd included as a random factor	834:902	The effect of sow, litter, and parturition factors on CY and CC were evaluated with a linear mixed regression model with herd included as a random factor.
26020907	1	10	theme	composition	174:184	arg1	characteristics					96:110	the main characteristics	87:110	the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs	87:197	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	8	11	theme	3,342	1017:1021	arg1	±					1023:1023	±	1023:1023	±	1023:1023	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	10	12	dep	115	1169:1171	arg1	to					1166:1167	to	1166:1167	to	1166:1167	In sows with a GL of 114 to 115 d, CY increased with higher LW ( = 0.009).
26020907	15	13	theme	litter	1864:1869	arg1	factors					1871:1877	different sow and litter factors	1846:1877	different sow and litter factors	1846:1877	In conclusion, this study demonstrated that CY and CF are affected by different sow and litter factors.
26020907	1	14	theme	colostrum	115:123	arg1	intake					125:130	colostrum intake	115:130	colostrum intake (CI)	115:135	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	1	14	theme	colostrum	115:123	arg1	CI					133:134	CI	133:134	CI	133:134	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	10	15	with	sows	1144:1147	arg1	GL					1156:1157	a GL	1154:1157	a GL of 114 to 115 d	1154:1173	In sows with a GL of 114 to 115 d, CY increased with higher LW ( = 0.009).
26020907	8	16	theme	107	1025:1027	arg1	±					1023:1023	±	1023:1023	±	1023:1023	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	6	17	theme	3,500	591:595	arg1	g					603:603	3,500 ± 110 g	591:603	3,500 ± 110 g	591:603	Colostrum yield averaged 3,500 ± 110 g and the percentage of colostral fat (CF), protein, and lactose in colostrum averaged 5.39 ± 0.12, 16.49 ± 0.14, and 2.02 ± 0.05 %, respectively.
26020907	15	18	theme	sow	1856:1858	arg1	factors					1871:1877	different sow and litter factors	1846:1877	different sow and litter factors	1846:1877	In conclusion, this study demonstrated that CY and CF are affected by different sow and litter factors.
26020907	0	19	theme	nutritional	55:65	arg1	composition					67:77	nutritional composition	55:77	nutritional composition	55:77	Sow and litter factors influencing colostrum yield and nutritional composition.
26020907	12	20	dep	±	1447:1447	arg1	0.001					1457:1461	0.001	1457:1461	0.001	1457:1461	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	1	21	theme	colostrum	138:146	arg1	CY					155:156	CY	155:156	CY	155:156	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	1	21	theme	colostrum	138:146	arg1	yield					148:152	colostrum yield	138:152	colostrum yield (CY)	138:157	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	16	22	theme	reproduction	1973:1984	arg1	potential					1986:1994	reproduction potential	1973:1994	reproduction potential	1973:1994	Pig producers may implement these observations in their management to maximize production or reproduction potential by optimizing CI, CY, and CC.
26020907	7	23	theme	factors	793:799	arg1	effect					754:759	The effect	750:759	The effect of sow, litter, and parturition factors on CY and CC	750:812	The effect of sow, litter, and parturition factors on CY and CC were evaluated with a linear mixed regression model with herd included as a random factor.
26020907	8	24	dep	115	1010:1012	arg1	to					1007:1008	to	1007:1008	to	1007:1008	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	13	25	dep	piglets	1630:1636	arg1	=					1640:1640	=	1640:1640	= 0.03	1640:1645	The percentage of CF was negatively associated with parity ( = 0.02) and positively associated with the number of live-born piglets ( = 0.03).
26020907	6	26	theme	colostral	627:635	arg1	CF					642:643	CF	642:643	CF	642:643	Colostrum yield averaged 3,500 ± 110 g and the percentage of colostral fat (CF), protein, and lactose in colostrum averaged 5.39 ± 0.12, 16.49 ± 0.14, and 2.02 ± 0.05 %, respectively.
26020907	6	26	theme	colostral	627:635	arg1	fat					637:639	colostral fat	627:639	colostral fat (CF)	627:644	Colostrum yield averaged 3,500 ± 110 g and the percentage of colostral fat (CF), protein, and lactose in colostrum averaged 5.39 ± 0.12, 16.49 ± 0.14, and 2.02 ± 0.05 %, respectively.
26020907	3	27	theme	breeds	360:365	arg1	sows					340:343	100 sows	336:343	100 sows of 5 different breeds	336:365	In total, 100 sows of 5 different breeds and their 1,455 live-born piglets were included.
26020907	3	27	theme	breeds	360:365	arg1	piglets					393:399	1,455 live-born piglets	377:399	their 1,455 live-born piglets	371:399	In total, 100 sows of 5 different breeds and their 1,455 live-born piglets were included.
26020907	5	28	theme	Colostrum	476:484	arg1	composition					486:496	Colostrum composition	476:496	Colostrum composition	476:496	Colostrum composition was analyzed by LactoScope Fourier transform infrared spectroscopy.
26020907	12	29	from	fat	1338:1340	arg1	colostrums					1345:1354	colostrums	1345:1354	colostrums	1345:1354	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	7	30	from	effect	754:759	arg1	CC					811:812	CC	811:812	CC	811:812	The effect of sow, litter, and parturition factors on CY and CC were evaluated with a linear mixed regression model with herd included as a random factor.
26020907	7	30	from	effect	754:759	arg1	CY					804:805	CY	804:805	CY	804:805	The effect of sow, litter, and parturition factors on CY and CC were evaluated with a linear mixed regression model with herd included as a random factor.
26020907	14	31	theme	colostral	1668:1676	arg1	protein					1678:1684	colostral protein	1668:1684	colostral protein	1668:1684	The percentages of colostral protein and lactose were not significantly associated with any factor in the multivariable model.
26020907	7	32	theme	litter	769:774	arg1	factors					793:799	sow, litter, and parturition factors	764:799	sow, litter, and parturition factors	764:799	The effect of sow, litter, and parturition factors on CY and CC were evaluated with a linear mixed regression model with herd included as a random factor.
26020907	12	33	from	percentage	1324:1333	arg1	colostrums					1345:1354	colostrums	1345:1354	colostrums	1345:1354	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	12	34	dep	Danbred	1469:1475	arg1	0.22					1485:1488	5.34 ± 0.22	1478:1488	5.34 ± 0.22	1478:1488	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	12	35	theme	fat	1338:1340	arg1	higher					1360:1365	higher	1360:1365	higher	1360:1365	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	12	35	theme	fat	1338:1340	arg1	percentage					1324:1333	The percentage	1320:1333	The percentage of fat in colostrums	1320:1354	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	3	36	theme	live-born	383:391	arg1	piglets					393:399	1,455 live-born piglets	377:399	their 1,455 live-born piglets	371:399	In total, 100 sows of 5 different breeds and their 1,455 live-born piglets were included.
26020907	0	37	theme	litter	8:13	arg1	factors					15:21	litter factors	8:21	litter factors	8:21	Sow and litter factors influencing colostrum yield and nutritional composition.
26020907	1	38	theme	characteristics	96:110	arg1	One					80:82	One	80:82	One	80:82	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	1	38	theme	characteristics	96:110	arg1	characteristics					96:110	the main characteristics	87:110	the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs	87:197	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	4	39	theme	piglets	467:473	arg1	CI					446:447	the CI	442:447	the CI of their suckling piglets	442:473	Sows' CY was estimated by the CI of their suckling piglets.
26020907	6	40	theme	±	726:726	arg1	%					733:733	2.02 ± 0.05 %	721:733	2.02 ± 0.05 %	721:733	Colostrum yield averaged 3,500 ± 110 g and the percentage of colostral fat (CF), protein, and lactose in colostrum averaged 5.39 ± 0.12, 16.49 ± 0.14, and 2.02 ± 0.05 %, respectively.
26020907	8	41	dep	=	1032:1032	arg1	g					1029:1029	3,342 ± 107 g	1017:1029	3,342 ± 107 g	1017:1029	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	12	42	dep	0.22	1485:1488	arg1	<					1491:1491	< 0.001	1491:1497	< 0.001	1491:1497	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	13	43	theme	live-born	1620:1628	arg1	piglets					1630:1636	live-born piglets	1620:1636	live-born piglets ( = 0.03)	1620:1646	The percentage of CF was negatively associated with parity ( = 0.02) and positively associated with the number of live-born piglets ( = 0.03).
26020907	12	44	theme	±	1387:1387	arg1	sows					1376:1379	Hypor sows	1370:1379	Hypor sows (6.35 ± 0.51)	1370:1393	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	12	44	theme	±	1387:1387	arg1	0.51					1389:1392	6.35 ± 0.51	1382:1392	6.35 ± 0.51	1382:1392	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	12	45	theme	6.35	1382:1385	arg1	±					1387:1387	±	1387:1387	±	1387:1387	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	5	46	dep	Fourier	525:531	arg1	transform					533:541	transform	533:541	transform infrared spectroscopy	533:563	Colostrum composition was analyzed by LactoScope Fourier transform infrared spectroscopy.
26020907	6	47	theme	protein	647:653	arg1	percentage					613:622	the percentage	609:622	the percentage of colostral fat (CF), protein, and lactose in colostrum	609:679	Colostrum yield averaged 3,500 ± 110 g and the percentage of colostral fat (CF), protein, and lactose in colostrum averaged 5.39 ± 0.12, 16.49 ± 0.14, and 2.02 ± 0.05 %, respectively.
26020907	1	48	from	characteristics	96:110	arg1	pigs					194:197	pigs	194:197	pigs	194:197	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	9	49	theme	litter	1078:1083	arg1	weight					1091:1096	the litter birth weight	1074:1096	the litter birth weight of suckling piglets (LW)	1074:1121	An interaction was found between the litter birth weight of suckling piglets (LW) and GL ( = 0.03).
26020907	9	49	theme	litter	1078:1083	arg1	LW					1119:1120	LW	1119:1120	LW	1119:1120	An interaction was found between the litter birth weight of suckling piglets (LW) and GL ( = 0.03).
26020907	14	50	from	factor	1741:1746	arg1	model					1769:1773	the multivariable model	1751:1773	the multivariable model	1751:1773	The percentages of colostral protein and lactose were not significantly associated with any factor in the multivariable model.
26020907	8	51	with	Sows	905:908	arg1	length					927:932	a gestation length	915:932	a gestation length (GL) of 113 d	915:946	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	8	51	with	Sows	905:908	arg1	GL					935:936	GL	935:936	GL	935:936	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	9	52	dep	=	1132:1132	arg1	0.03					1134:1137	0.03	1134:1137	0.03	1134:1137	An interaction was found between the litter birth weight of suckling piglets (LW) and GL ( = 0.03).
26020907	2	53	theme	commercial	308:317	arg1	herds					319:323	10 commercial herds	305:323	10 commercial herds	305:323	The present observational study aimed to investigate factors influencing CY and CC in 10 commercial herds.
26020907	10	54	theme	d	1173:1173	arg1	GL					1156:1157	a GL	1154:1157	a GL of 114 to 115 d	1154:1173	In sows with a GL of 114 to 115 d, CY increased with higher LW ( = 0.009).
26020907	11	55	theme	first	1253:1257	arg1	suckling					1259:1266	first suckling	1253:1266	first suckling	1253:1266	A shorter interval between birth and first suckling of the litter was related to a higher CY ( < 0.01).
26020907	9	56	theme	suckling	1101:1108	arg1	piglets					1110:1116	suckling piglets	1101:1116	suckling piglets	1101:1116	An interaction was found between the litter birth weight of suckling piglets (LW) and GL ( = 0.03).
26020907	12	57	theme	±	1483:1483	arg1	0.22					1485:1488	5.34 ± 0.22	1478:1488	5.34 ± 0.22	1478:1488	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	12	58	from	colostrums	1345:1354	arg1	higher					1360:1365	higher	1360:1365	higher	1360:1365	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	12	58	from	colostrums	1345:1354	arg1	percentage					1324:1333	The percentage	1320:1333	The percentage of fat in colostrums	1320:1354	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	2	59	theme	observational	231:243	arg1	study					245:249	The present observational study	219:249	The present observational study	219:249	The present observational study aimed to investigate factors influencing CY and CC in 10 commercial herds.
26020907	6	60	from	percentage	613:622	arg1	colostrum					671:679	colostrum	671:679	colostrum	671:679	Colostrum yield averaged 3,500 ± 110 g and the percentage of colostral fat (CF), protein, and lactose in colostrum averaged 5.39 ± 0.12, 16.49 ± 0.14, and 2.02 ± 0.05 %, respectively.
26020907	12	61	dep	PIC	1403:1405	arg1	=					1421:1421	=	1421:1421	= 0.001	1421:1427	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	11	62	theme	litter	1275:1280	arg1	birth					1243:1247	birth	1243:1247	birth	1243:1247	A shorter interval between birth and first suckling of the litter was related to a higher CY ( < 0.01).
26020907	11	62	theme	litter	1275:1280	arg1	suckling					1259:1266	first suckling	1253:1266	first suckling	1253:1266	A shorter interval between birth and first suckling of the litter was related to a higher CY ( < 0.01).
26020907	8	63	contain	had	948:950	arg2	CY					961:962	a higher CY	952:962	a higher CY (4,178 ± 506 g)	952:978	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	8	63	contain	had	948:950	arg1	Sows					905:908	Sows	905:908	Sows with a gestation length (GL) of 113 d	905:946	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	8	63	contain	had	948:950	arg2	g					977:977	4,178 ± 506 g	965:977	4,178 ± 506 g	965:977	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	1	64	theme	yield	148:152	arg1	characteristics					96:110	the main characteristics	87:110	the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs	87:197	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	8	65	theme	higher	954:959	arg1	g					977:977	4,178 ± 506 g	965:977	4,178 ± 506 g	965:977	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	8	65	theme	higher	954:959	arg1	CY					961:962	a higher CY	952:962	a higher CY (4,178 ± 506 g)	952:978	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	16	66	theme	Pig	1880:1882	arg1	producers					1884:1892	Pig producers	1880:1892	Pig producers	1880:1892	Pig producers may implement these observations in their management to maximize production or reproduction potential by optimizing CI, CY, and CC.
26020907	12	67	dep	=	1421:1421	arg1	±					1413:1413	4.98 ± 0.27	1408:1418	4.98 ± 0.27	1408:1418	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	8	68	theme	gestation	917:925	arg1	length					927:932	a gestation length	915:932	a gestation length (GL) of 113 d	915:946	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	8	68	theme	gestation	917:925	arg1	GL					935:936	GL	935:936	GL	935:936	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	7	69	theme	random	890:895	arg1	factor					897:902	a random factor	888:902	a random factor	888:902	The effect of sow, litter, and parturition factors on CY and CC were evaluated with a linear mixed regression model with herd included as a random factor.
26020907	9	70	theme	=	1132:1132	arg1	GL					1127:1128	GL	1127:1128	GL ( = 0.03)	1127:1138	An interaction was found between the litter birth weight of suckling piglets (LW) and GL ( = 0.03).
26020907	8	71	theme	±	971:971	arg1	g					977:977	4,178 ± 506 g	965:977	4,178 ± 506 g	965:977	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	8	71	theme	±	971:971	arg1	CY					961:962	a higher CY	952:962	a higher CY (4,178 ± 506 g)	952:978	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	12	72	dep	Topigs	1431:1436	arg1	±					1447:1447	5.05 ± 0.14	1442:1452	5.05 ± 0.14	1442:1452	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	1	73	theme	colostrum	164:172	arg1	CC					187:188	CC	187:188	CC	187:188	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	1	73	theme	colostrum	164:172	arg1	composition					174:184	colostrum composition	164:184	colostrum composition (CC)	164:189	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	7	74	theme	regression	849:858	arg1	model					860:864	a linear mixed regression model	834:864	a linear mixed regression model with herd included as a random factor	834:902	The effect of sow, litter, and parturition factors on CY and CC were evaluated with a linear mixed regression model with herd included as a random factor.
26020907	6	75	theme	±	597:597	arg1	g					603:603	3,500 ± 110 g	591:603	3,500 ± 110 g	591:603	Colostrum yield averaged 3,500 ± 110 g and the percentage of colostral fat (CF), protein, and lactose in colostrum averaged 5.39 ± 0.12, 16.49 ± 0.14, and 2.02 ± 0.05 %, respectively.
26020907	13	76	theme	CF	1524:1525	arg1	percentage					1510:1519	The percentage	1506:1519	The percentage of CF	1506:1525	The percentage of CF was negatively associated with parity ( = 0.02) and positively associated with the number of live-born piglets ( = 0.03).
26020907	7	77	theme	linear	836:841	arg1	model					860:864	a linear mixed regression model	834:864	a linear mixed regression model with herd included as a random factor	834:902	The effect of sow, litter, and parturition factors on CY and CC were evaluated with a linear mixed regression model with herd included as a random factor.
26020907	1	78	theme	intake	125:130	arg1	characteristics					96:110	the main characteristics	87:110	the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs	87:197	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	8	79	with	sows	985:988	arg1	GL					997:998	a GL	995:998	a GL of 114 to 115 d (3,342 ± 107 g; = 0.04)	995:1038	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	6	80	theme	Colostrum	566:574	arg1	yield					576:580	Colostrum yield	566:580	Colostrum yield	566:580	Colostrum yield averaged 3,500 ± 110 g and the percentage of colostral fat (CF), protein, and lactose in colostrum averaged 5.39 ± 0.12, 16.49 ± 0.14, and 2.02 ± 0.05 %, respectively.
26020907	15	81	theme	different	1846:1854	arg1	factors					1871:1877	different sow and litter factors	1846:1877	different sow and litter factors	1846:1877	In conclusion, this study demonstrated that CY and CF are affected by different sow and litter factors.
26020907	12	82	theme	Topigs	1431:1436	arg1	sows					1500:1503	PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows	1403:1503	PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows	1403:1503	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	5	83	theme	LactoScope	514:523	arg1	Fourier					525:531	LactoScope Fourier	514:531	LactoScope Fourier transform infrared spectroscopy	514:563	Colostrum composition was analyzed by LactoScope Fourier transform infrared spectroscopy.
26020907	8	84	dep	d	1014:1014	arg1	=					1032:1032	=	1032:1032	= 0.04	1032:1037	Sows with a gestation length (GL) of 113 d had a higher CY (4,178 ± 506 g) than sows with a GL of 114 to 115 d (3,342 ± 107 g; = 0.04).
26020907	6	85	theme	fat	637:639	arg1	percentage					613:622	the percentage	609:622	the percentage of colostral fat (CF), protein, and lactose in colostrum	609:679	Colostrum yield averaged 3,500 ± 110 g and the percentage of colostral fat (CF), protein, and lactose in colostrum averaged 5.39 ± 0.12, 16.49 ± 0.14, and 2.02 ± 0.05 %, respectively.
26020907	14	86	theme	lactose	1690:1696	arg1	percentages					1653:1663	The percentages	1649:1663	The percentages of colostral protein and lactose	1649:1696	The percentages of colostral protein and lactose were not significantly associated with any factor in the multivariable model.
26020907	7	87	with	model	860:864	arg1	herd					871:874	herd	871:874	herd included as a random factor	871:902	The effect of sow, litter, and parturition factors on CY and CC were evaluated with a linear mixed regression model with herd included as a random factor.
26020907	14	88	theme	protein	1678:1684	arg1	percentages					1653:1663	The percentages	1649:1663	The percentages of colostral protein and lactose	1649:1696	The percentages of colostral protein and lactose were not significantly associated with any factor in the multivariable model.
26020907	7	89	theme	parturition	781:791	arg1	factors					793:799	sow, litter, and parturition factors	764:799	sow, litter, and parturition factors	764:799	The effect of sow, litter, and parturition factors on CY and CC were evaluated with a linear mixed regression model with herd included as a random factor.
26020907	4	90	theme	suckling	458:465	arg1	piglets					467:473	their suckling piglets	452:473	their suckling piglets	452:473	Sows' CY was estimated by the CI of their suckling piglets.
26020907	3	91	theme	different	350:358	arg1	breeds					360:365	5 different breeds	348:365	5 different breeds	348:365	In total, 100 sows of 5 different breeds and their 1,455 live-born piglets were included.
26020907	12	92	theme	PIC	1403:1405	arg1	sows					1500:1503	PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows	1403:1503	PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows	1403:1503	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	1	93	theme	main	91:94	arg1	characteristics					96:110	the main characteristics	87:110	the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs	87:197	One of the main characteristics of colostrum intake (CI), colostrum yield (CY), and colostrum composition (CC) in pigs is its variability.
26020907	0	94	theme	colostrum	35:43	arg1	yield					45:49	colostrum yield	35:49	colostrum yield	35:49	Sow and litter factors influencing colostrum yield and nutritional composition.
26020907	6	95	theme	lactose	660:666	arg1	percentage					613:622	the percentage	609:622	the percentage of colostral fat (CF), protein, and lactose in colostrum	609:679	Colostrum yield averaged 3,500 ± 110 g and the percentage of colostral fat (CF), protein, and lactose in colostrum averaged 5.39 ± 0.12, 16.49 ± 0.14, and 2.02 ± 0.05 %, respectively.
26020907	12	96	theme	Hypor	1370:1374	arg1	sows					1376:1379	Hypor sows	1370:1379	Hypor sows (6.35 ± 0.51)	1370:1393	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	12	96	theme	Hypor	1370:1374	arg1	0.51					1389:1392	6.35 ± 0.51	1382:1392	6.35 ± 0.51	1382:1392	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
26020907	7	97	theme	sow	764:766	arg1	factors					793:799	sow, litter, and parturition factors	764:799	sow, litter, and parturition factors	764:799	The effect of sow, litter, and parturition factors on CY and CC were evaluated with a linear mixed regression model with herd included as a random factor.
26020907	13	98	theme	piglets	1630:1636	arg1	number					1610:1615	the number	1606:1615	the number of live-born piglets ( = 0.03)	1606:1646	The percentage of CF was negatively associated with parity ( = 0.02) and positively associated with the number of live-born piglets ( = 0.03).
26020907	5	99	dep	transform	533:541	arg1	infrared					543:550	infrared	543:550	transform infrared spectroscopy	533:563	Colostrum composition was analyzed by LactoScope Fourier transform infrared spectroscopy.
26020907	13	100	dep	parity	1558:1563	arg1	=					1567:1567	=	1567:1567	= 0.02	1567:1572	The percentage of CF was negatively associated with parity ( = 0.02) and positively associated with the number of live-born piglets ( = 0.03).
26020907	11	101	theme	higher	1299:1304	arg1	CY					1306:1307	a higher CY	1297:1307	a higher CY ( < 0.01)	1297:1317	A shorter interval between birth and first suckling of the litter was related to a higher CY ( < 0.01).
26020907	11	101	theme	higher	1299:1304	arg1	<					1311:1311	< 0.01	1311:1316	< 0.01	1311:1316	A shorter interval between birth and first suckling of the litter was related to a higher CY ( < 0.01).
26020907	11	102	theme	shorter	1218:1224	arg1	related					1286:1292	related	1286:1292	related	1286:1292	A shorter interval between birth and first suckling of the litter was related to a higher CY ( < 0.01).
26020907	11	102	theme	shorter	1218:1224	arg1	interval					1226:1233	A shorter interval	1216:1233	A shorter interval between birth and first suckling of the litter	1216:1280	A shorter interval between birth and first suckling of the litter was related to a higher CY ( < 0.01).
26020907	9	103	theme	birth	1085:1089	arg1	weight					1091:1096	the litter birth weight	1074:1096	the litter birth weight of suckling piglets (LW)	1074:1121	An interaction was found between the litter birth weight of suckling piglets (LW) and GL ( = 0.03).
26020907	9	103	theme	birth	1085:1089	arg1	LW					1119:1120	LW	1119:1120	LW	1119:1120	An interaction was found between the litter birth weight of suckling piglets (LW) and GL ( = 0.03).
26020907	12	104	theme	5.34	1478:1481	arg1	±					1483:1483	±	1483:1483	±	1483:1483	The percentage of fat in colostrums was higher in Hypor sows (6.35 ± 0.51) than in PIC (4.98 ± 0.27; = 0.001), Topigs 20 (5.05 ± 0.14; < 0.001), and Danbred (5.34 ± 0.22; < 0.001) sows.
25863599	6	0	theme	bags	1122:1125	arg1	types					1113:1117	both types	1108:1117	both types of bags	1108:1125	We found a slight variation in anti-Xa activity when infused over 24hours for both types of bags, with and without in-line filtration, showing that heparin remains stable during this infusion period in both BPN admixtures and G50%.
25863599	3	1	theme	line	724:727	arg1	outlet					701:706	the outlet	697:706	the outlet of the infusion line	697:727	The anti-Xa activity of heparin was measured in bags at T0, after the 24-hour infusion and in eluates at the outlet of the infusion line after 24hours, using a validated chromogenic anti-Xa method.
25863599	2	2	theme	bags	414:417	arg1	types					405:409	Two types	401:409	Two types of bags	401:417	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	6	3	from	period	1222:1227	arg1	admixtures					1241:1250	both BPN admixtures	1232:1250	both BPN admixtures	1232:1250	We found a slight variation in anti-Xa activity when infused over 24hours for both types of bags, with and without in-line filtration, showing that heparin remains stable during this infusion period in both BPN admixtures and G50%.
25863599	1	4	theme	50	379:380	arg1	%					381:381	%	381:381	%	381:381	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	6	5	from	%	1259:1259	arg1	admixtures					1241:1250	both BPN admixtures	1232:1250	both BPN admixtures	1232:1250	We found a slight variation in anti-Xa activity when infused over 24hours for both types of bags, with and without in-line filtration, showing that heparin remains stable during this infusion period in both BPN admixtures and G50%.
25863599	4	6	theme	Student	909:915	arg1	t-test					917:922	the Student t-test	905:922	the Student t-test	905:922	Comparisons of the mean concentration observed with the theoretical value for anti-Xa activity were performed with the Student t-test.
25863599	0	7	theme	premature	95:103	arg1	neonates					105:112	premature neonates	95:112	premature neonates	95:112	Assessment of anti-factor Xa activity of heparin in binary parenteral nutrition admixtures for premature neonates.
25863599	0	8	from	Assessment	0:9	arg1	nutrition					70:78	binary parenteral nutrition	52:78	binary parenteral nutrition	52:78	Assessment of anti-factor Xa activity of heparin in binary parenteral nutrition admixtures for premature neonates.
25863599	2	9	theme	sodium	497:502	arg1	heparin					504:510	sodium heparin	497:510	sodium heparin	497:510	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	1	10	theme	%	381:381	arg1	solution					391:398	a 50% glucose solution	377:398	a 50% glucose solution	377:398	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	3	11	theme	24-hour	662:668	arg1	infusion					670:677	the 24-hour infusion	658:677	the 24-hour infusion	658:677	The anti-Xa activity of heparin was measured in bags at T0, after the 24-hour infusion and in eluates at the outlet of the infusion line after 24hours, using a validated chromogenic anti-Xa method.
25863599	2	12	dep	admixtures	442:451	arg1	1					435:435	1	435:435	1	435:435	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	2	12	dep	admixtures	442:451	arg1	defined					466:472	defined	466:472	defined in the NICU	466:484	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	1	13	theme	24-hour	316:322	arg1	infusion					324:331	a 24-hour infusion	314:331	a 24-hour infusion	314:331	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	1	14	theme	glucose	383:389	arg1	solution					391:398	a 50% glucose solution	377:398	a 50% glucose solution	377:398	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	1	15	theme	premature	246:254	arg1	neonates					256:263	premature neonates	246:263	premature neonates	246:263	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	3	16	theme	anti-Xa	596:602	arg1	activity					604:611	The anti-Xa activity	592:611	The anti-Xa activity of heparin	592:622	The anti-Xa activity of heparin was measured in bags at T0, after the 24-hour infusion and in eluates at the outlet of the infusion line after 24hours, using a validated chromogenic anti-Xa method.
25863599	2	17	with	%	556:556	arg1	heparin					570:576	sodium heparin	563:576	sodium heparin	563:576	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	5	18	theme	activity	948:955	arg1	values					930:935	Mean values	925:935	Mean values of anti-Xa activity	925:955	Mean values of anti-Xa activity do not differ significantly from the values expected for all conditions.
25863599	1	19	dep	in	118:119	arg1	vitro					121:125	vitro	121:125	vitro	121:125	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	5	20	theme	anti-Xa	940:946	arg1	activity					948:955	anti-Xa activity	940:955	anti-Xa activity	940:955	Mean values of anti-Xa activity do not differ significantly from the values expected for all conditions.
25863599	4	21	theme	anti-Xa	868:874	arg1	activity					876:883	anti-Xa activity	868:883	anti-Xa activity	868:883	Comparisons of the mean concentration observed with the theoretical value for anti-Xa activity were performed with the Student t-test.
25863599	4	22	theme	concentration	814:826	arg1	Comparisons					790:800	Comparisons	790:800	Comparisons of the mean concentration observed with the theoretical value for anti-Xa activity	790:883	Comparisons of the mean concentration observed with the theoretical value for anti-Xa activity were performed with the Student t-test.
25863599	4	23	theme	mean	809:812	arg1	concentration					814:826	the mean concentration	805:826	the mean concentration observed with the theoretical value for anti-Xa activity	805:883	Comparisons of the mean concentration observed with the theoretical value for anti-Xa activity were performed with the Student t-test.
25863599	1	24	with	interaction	360:370	arg1	solution					391:398	a 50% glucose solution	377:398	a 50% glucose solution	377:398	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	1	25	theme	anti-Xa	166:172	arg1	activity					174:181	the anti-Xa activity	162:181	the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates	162:263	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	3	26	theme	heparin	616:622	arg1	activity					604:611	The anti-Xa activity	592:611	The anti-Xa activity of heparin	592:622	The anti-Xa activity of heparin was measured in bags at T0, after the 24-hour infusion and in eluates at the outlet of the infusion line after 24hours, using a validated chromogenic anti-Xa method.
25863599	3	27	theme	chromogenic	762:772	arg1	method					782:787	a validated chromogenic anti-Xa method	750:787	a validated chromogenic anti-Xa method	750:787	The anti-Xa activity of heparin was measured in bags at T0, after the 24-hour infusion and in eluates at the outlet of the infusion line after 24hours, using a validated chromogenic anti-Xa method.
25863599	0	28	theme	Xa	26:27	arg1	activity					29:36	anti-factor Xa activity	14:36	anti-factor Xa activity of heparin	14:47	Assessment of anti-factor Xa activity of heparin in binary parenteral nutrition admixtures for premature neonates.
25863599	3	29	theme	anti-Xa	774:780	arg1	method					782:787	a validated chromogenic anti-Xa method	750:787	a validated chromogenic anti-Xa method	750:787	The anti-Xa activity of heparin was measured in bags at T0, after the 24-hour infusion and in eluates at the outlet of the infusion line after 24hours, using a validated chromogenic anti-Xa method.
25863599	2	30	theme	BPN	438:440	arg1	heparin					504:510	sodium heparin	497:510	sodium heparin	497:510	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	2	30	theme	BPN	438:440	arg1	admixtures					442:451	BPN admixtures	438:451	(1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL	434:522	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	0	31	theme	anti-factor	14:24	arg1	Xa					26:27	anti-factor Xa	14:27	anti-factor Xa activity of heparin	14:47	Assessment of anti-factor Xa activity of heparin in binary parenteral nutrition admixtures for premature neonates.
25863599	4	32	theme	theoretical	846:856	arg1	value					858:862	the theoretical value	842:862	the theoretical value for anti-Xa activity	842:883	Comparisons of the mean concentration observed with the theoretical value for anti-Xa activity were performed with the Student t-test.
25863599	3	33	from	outlet	701:706	arg1	T0					648:649	T0	648:649	T0	648:649	The anti-Xa activity of heparin was measured in bags at T0, after the 24-hour infusion and in eluates at the outlet of the infusion line after 24hours, using a validated chromogenic anti-Xa method.
25863599	1	34	theme	heparin	186:192	arg1	activity					174:181	the anti-Xa activity	162:181	the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates	162:263	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	1	35	theme	neonatal	272:279	arg1	NICU					302:305	NICU	302:305	NICU	302:305	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	1	35	theme	neonatal	272:279	arg1	unit					296:299	our neonatal intensive care unit (NICU)	268:306	our neonatal intensive care unit (NICU)	268:306	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	6	36	from	variation	1048:1056	arg1	activity					1069:1076	anti-Xa activity	1061:1076	anti-Xa activity	1061:1076	We found a slight variation in anti-Xa activity when infused over 24hours for both types of bags, with and without in-line filtration, showing that heparin remains stable during this infusion period in both BPN admixtures and G50%.
25863599	3	37	theme	validated	752:760	arg1	method					782:787	a validated chromogenic anti-Xa method	750:787	a validated chromogenic anti-Xa method	750:787	The anti-Xa activity of heparin was measured in bags at T0, after the 24-hour infusion and in eluates at the outlet of the infusion line after 24hours, using a validated chromogenic anti-Xa method.
25863599	0	38	theme	activity	29:36	arg1	Assessment					0:9	Assessment	0:9	Assessment of anti-factor Xa activity of heparin in binary parenteral nutrition	0:78	Assessment of anti-factor Xa activity of heparin in binary parenteral nutrition admixtures for premature neonates.
25863599	1	39	theme	intensive	281:289	arg1	NICU					302:305	NICU	302:305	NICU	302:305	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	1	39	theme	intensive	281:289	arg1	unit					296:299	our neonatal intensive care unit (NICU)	268:306	our neonatal intensive care unit (NICU)	268:306	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	6	40	theme	BPN	1237:1239	arg1	admixtures					1241:1250	both BPN admixtures	1232:1250	both BPN admixtures	1232:1250	We found a slight variation in anti-Xa activity when infused over 24hours for both types of bags, with and without in-line filtration, showing that heparin remains stable during this infusion period in both BPN admixtures and G50%.
25863599	5	41	theme	Mean	925:928	arg1	values					930:935	Mean values	925:935	Mean values of anti-Xa activity	925:955	Mean values of anti-Xa activity do not differ significantly from the values expected for all conditions.
25863599	1	42	theme	binary	197:202	arg1	BPN					226:228	BPN	226:228	BPN	226:228	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	1	42	theme	binary	197:202	arg1	nutrition					215:223	binary parenteral nutrition	197:223	binary parenteral nutrition (BPN) admixtures for premature neonates	197:263	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	1	43	theme	care	291:294	arg1	NICU					302:305	NICU	302:305	NICU	302:305	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	1	43	theme	care	291:294	arg1	unit					296:299	our neonatal intensive care unit (NICU)	268:306	our neonatal intensive care unit (NICU)	268:306	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	6	44	theme	infusion	1213:1220	arg1	period					1222:1227	this infusion period	1208:1227	this infusion period in both BPN admixtures	1208:1250	We found a slight variation in anti-Xa activity when infused over 24hours for both types of bags, with and without in-line filtration, showing that heparin remains stable during this infusion period in both BPN admixtures and G50%.
25863599	6	45	theme	anti-Xa	1061:1067	arg1	activity					1069:1076	anti-Xa activity	1061:1076	anti-Xa activity	1061:1076	We found a slight variation in anti-Xa activity when infused over 24hours for both types of bags, with and without in-line filtration, showing that heparin remains stable during this infusion period in both BPN admixtures and G50%.
25863599	0	46	theme	heparin	41:47	arg1	activity					29:36	anti-factor Xa activity	14:36	anti-factor Xa activity of heparin	14:47	Assessment of anti-factor Xa activity of heparin in binary parenteral nutrition admixtures for premature neonates.
25863599	1	47	theme	drug	355:358	arg1	interaction					360:370	drug interaction	355:370	drug interaction with a 50% glucose solution	355:398	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	1	48	theme	in	118:119	arg1	study					127:131	An in vitro study	115:131	An in vitro study	115:131	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	2	49	theme	sodium	563:568	arg1	heparin					570:576	sodium heparin	563:576	sodium heparin	563:576	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	6	50	theme	in-line	1145:1151	arg1	filtration					1153:1162	in-line filtration	1145:1162	in-line filtration	1145:1162	We found a slight variation in anti-Xa activity when infused over 24hours for both types of bags, with and without in-line filtration, showing that heparin remains stable during this infusion period in both BPN admixtures and G50%.
25863599	1	51	theme	parenteral	204:213	arg1	BPN					226:228	BPN	226:228	BPN	226:228	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	1	51	theme	parenteral	204:213	arg1	nutrition					215:223	binary parenteral nutrition	197:223	binary parenteral nutrition (BPN) admixtures for premature neonates	197:263	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	2	52	dep	prepared	424:431	arg1	bags					532:535	(2) bags	528:535	(2) bags containing only G50% with sodium heparin at 193 UI/mL	528:589	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	2	52	dep	prepared	424:431	arg1	heparin					504:510	sodium heparin	497:510	sodium heparin	497:510	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	2	52	dep	prepared	424:431	arg1	admixtures					442:451	BPN admixtures	438:451	(1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL	434:522	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	6	53	theme	slight	1041:1046	arg1	variation					1048:1056	a slight variation	1039:1056	a slight variation in anti-Xa activity when infused over 24hours for both types of bags	1039:1125	We found a slight variation in anti-Xa activity when infused over 24hours for both types of bags, with and without in-line filtration, showing that heparin remains stable during this infusion period in both BPN admixtures and G50%.
25863599	1	54	theme	nutrition	215:223	arg1	admixtures					231:240	binary parenteral nutrition (BPN) admixtures	197:240	binary parenteral nutrition (BPN) admixtures for premature neonates	197:263	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	0	55	theme	parenteral	59:68	arg1	nutrition					70:78	binary parenteral nutrition	52:78	binary parenteral nutrition	52:78	Assessment of anti-factor Xa activity of heparin in binary parenteral nutrition admixtures for premature neonates.
25863599	1	56	from	activity	174:181	arg1	admixtures					231:240	binary parenteral nutrition (BPN) admixtures	197:240	binary parenteral nutrition (BPN) admixtures for premature neonates	197:263	An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution.
25863599	2	57	from	UI/mL	518:522	arg1	bags					532:535	(2) bags	528:535	(2) bags containing only G50% with sodium heparin at 193 UI/mL	528:589	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	2	57	from	UI/mL	518:522	arg1	heparin					504:510	sodium heparin	497:510	sodium heparin	497:510	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	2	57	from	UI/mL	518:522	arg1	admixtures					442:451	BPN admixtures	438:451	(1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL	434:522	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	0	58	theme	binary	52:57	arg1	nutrition					70:78	binary parenteral nutrition	52:78	binary parenteral nutrition	52:78	Assessment of anti-factor Xa activity of heparin in binary parenteral nutrition admixtures for premature neonates.
25863599	2	59	contain	containing	537:546	arg1	bags					532:535	(2) bags	528:535	(2) bags containing only G50% with sodium heparin at 193 UI/mL	528:589	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	2	59	contain	containing	537:546	arg1	heparin					504:510	sodium heparin	497:510	sodium heparin	497:510	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	2	59	contain	containing	537:546	arg2	%					556:556	only G50%	548:556	only G50% with sodium heparin	548:576	Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL.
25863599	3	60	theme	infusion	715:722	arg1	line					724:727	the infusion line	711:727	the infusion line	711:727	The anti-Xa activity of heparin was measured in bags at T0, after the 24-hour infusion and in eluates at the outlet of the infusion line after 24hours, using a validated chromogenic anti-Xa method.
28799654	11	0	theme	bean	1469:1472	arg1	composition					1483:1493	bean chemical composition	1469:1493	bean chemical composition	1469:1493	Therefore, bean chemical composition may serve as an indicator for beans extrusion behaviour and could be useful in selection of beans for extrusion.
28799654	11	0	theme	bean	1469:1472	arg1	indicator					1511:1519	an indicator	1508:1519	an indicator for beans extrusion behaviour	1508:1549	Therefore, bean chemical composition may serve as an indicator for beans extrusion behaviour and could be useful in selection of beans for extrusion.
28799654	4	1	from	relationships	545:557	arg1	properties					630:639	the extrudate properties	616:639	the extrudate properties	616:639	The relationships between bean characteristics and extrusion conditions on the extrudate properties were analysed.
28799654	9	2	theme	Similar	1260:1266	arg1	trends					1268:1273	Similar trends	1260:1273	Similar trends	1260:1273	Similar trends were observed in principal component analysis.
28799654	11	3	theme	chemical	1474:1481	arg1	composition					1483:1493	bean chemical composition	1469:1493	bean chemical composition	1469:1493	Therefore, bean chemical composition may serve as an indicator for beans extrusion behaviour and could be useful in selection of beans for extrusion.
28799654	11	3	theme	chemical	1474:1481	arg1	indicator					1511:1519	an indicator	1508:1519	an indicator for beans extrusion behaviour	1508:1549	Therefore, bean chemical composition may serve as an indicator for beans extrusion behaviour and could be useful in selection of beans for extrusion.
28799654	2	4	with	association	336:346	arg1	material					357:364	raw material characteristics and extrusion conditions	353:405	material	357:364	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	2	4	with	association	336:346	arg1	conditions					396:405	extrusion conditions	386:405	extrusion conditions	386:405	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	8	5	theme	final	1231:1235	arg1	viscosity					1237:1245	final viscosity	1231:1245	final viscosity	1231:1245	Iron and dietary fibre showed positive correlation while total ash exhibited negative correlation with peak viscosity, final viscosity and setback.
28799654	8	6	with	correlation	1198:1208	arg1	setback					1251:1257	setback	1251:1257	setback	1251:1257	Iron and dietary fibre showed positive correlation while total ash exhibited negative correlation with peak viscosity, final viscosity and setback.
28799654	8	6	with	correlation	1198:1208	arg1	viscosity					1220:1228	peak viscosity	1215:1228	peak viscosity	1215:1228	Iron and dietary fibre showed positive correlation while total ash exhibited negative correlation with peak viscosity, final viscosity and setback.
28799654	8	6	with	correlation	1198:1208	arg1	viscosity					1237:1245	final viscosity	1231:1245	final viscosity	1231:1245	Iron and dietary fibre showed positive correlation while total ash exhibited negative correlation with peak viscosity, final viscosity and setback.
28799654	4	7	theme	extrudate	620:628	arg1	properties					630:639	the extrudate properties	616:639	the extrudate properties	616:639	The relationships between bean characteristics and extrusion conditions on the extrudate properties were analysed.
28799654	9	8	theme	component	1302:1310	arg1	analysis					1312:1319	principal component analysis	1292:1319	principal component analysis	1292:1319	Similar trends were observed in principal component analysis.
28799654	10	9	dep	beans	1404:1408	arg1	starch					1419:1424	starch	1419:1424	starch	1419:1424	CONCLUSION Extrudate physico-chemical properties were found to be associated with beans protein, starch, iron, zinc and fibre contents.
28799654	10	9	dep	beans	1404:1408	arg1	contents					1448:1455	fibre contents	1442:1455	fibre contents	1442:1455	CONCLUSION Extrudate physico-chemical properties were found to be associated with beans protein, starch, iron, zinc and fibre contents.
28799654	10	9	dep	beans	1404:1408	arg1	iron					1427:1430	iron	1427:1430	iron	1427:1430	CONCLUSION Extrudate physico-chemical properties were found to be associated with beans protein, starch, iron, zinc and fibre contents.
28799654	10	9	dep	beans	1404:1408	arg1	beans					1404:1408	beans	1404:1408	beans protein, starch, iron, zinc and fibre contents	1404:1455	CONCLUSION Extrudate physico-chemical properties were found to be associated with beans protein, starch, iron, zinc and fibre contents.
28799654	10	9	dep	beans	1404:1408	arg1	protein					1410:1416	protein	1410:1416	protein	1410:1416	CONCLUSION Extrudate physico-chemical properties were found to be associated with beans protein, starch, iron, zinc and fibre contents.
28799654	10	9	dep	beans	1404:1408	arg1	zinc					1433:1436	zinc	1433:1436	zinc	1433:1436	CONCLUSION Extrudate physico-chemical properties were found to be associated with beans protein, starch, iron, zinc and fibre contents.
28799654	12	10	theme	Chemical	1626:1633	arg1	Industry					1635:1642	Chemical Industry	1626:1642	Chemical Industry	1626:1642	© 2017 Society of Chemical Industry.
28799654	2	11	theme	raw	353:355	arg1	material					357:364	raw material characteristics and extrusion conditions	353:405	material	357:364	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	7	12	theme	positive	953:960	arg1	P < 0.05					975:982	P < 0.05	975:982	P < 0.05	975:982	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	7	12	theme	positive	953:960	arg1	correlation					962:972	significant positive correlation	941:972	significant positive correlation (P < 0.05) with water solubility index	941:1011	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	6	13	theme	water	788:792	arg1	solubility					794:803	water solubility	788:803	water solubility	788:803	Expansion ratio and water solubility decreased, while bulk density, water absorption, peak and breakdown viscosities increased as feed moisture increased.
28799654	3	14	theme	common	511:516	arg1	beans					518:522	RWR2245 common beans	503:522	RWR2245 common beans	503:522	In this study, physico-chemical properties of raw and extruded Bishaz, K131, NABE19, Roba1 and RWR2245 common beans were determined.
28799654	7	15	theme	significant	941:951	arg1	P < 0.05					975:982	P < 0.05	975:982	P < 0.05	975:982	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	7	15	theme	significant	941:951	arg1	correlation					962:972	significant positive correlation	941:972	significant positive correlation (P < 0.05) with water solubility index	941:1011	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	6	16	theme	breakdown	863:871	arg1	viscosities					873:883	bulk density, water absorption, peak and breakdown viscosities	822:883	bulk density, water absorption, peak and breakdown viscosities	822:883	Expansion ratio and water solubility decreased, while bulk density, water absorption, peak and breakdown viscosities increased as feed moisture increased.
28799654	2	17	theme	bean	311:314	arg1	varieties					316:324	different bean varieties	301:324	different bean varieties	301:324	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	3	18	theme	Bishaz	471:476	arg1	properties					440:449	physico-chemical properties	423:449	physico-chemical properties of raw and extruded Bishaz, K131, NABE19, Roba1 and RWR2245 common beans	423:522	In this study, physico-chemical properties of raw and extruded Bishaz, K131, NABE19, Roba1 and RWR2245 common beans were determined.
28799654	1	19	theme	nutritious	194:203	arg1	products					220:227	highly nutritious and functional products	187:227	highly nutritious and functional products	187:227	BACKGROUND Extrusion processing offers the possibility of processing common beans industrially into highly nutritious and functional products.
28799654	3	20	theme	raw	454:456	arg1	properties					440:449	physico-chemical properties	423:449	physico-chemical properties of raw and extruded Bishaz, K131, NABE19, Roba1 and RWR2245 common beans	423:522	In this study, physico-chemical properties of raw and extruded Bishaz, K131, NABE19, Roba1 and RWR2245 common beans were determined.
28799654	7	21	theme	pasting	1091:1097	arg1	viscosities					1099:1109	pasting viscosities	1091:1109	pasting viscosities	1091:1109	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	7	22	theme	negative	1018:1025	arg1	P < 0.05					1041:1048	P < 0.05	1041:1048	P < 0.05	1041:1048	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	7	22	theme	negative	1018:1025	arg1	correlations					1027:1038	negative correlations	1018:1038	negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities	1018:1109	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	1	23	theme	BACKGROUND	87:96	arg1	processing					108:117	BACKGROUND Extrusion processing	87:117	BACKGROUND Extrusion processing	87:117	BACKGROUND Extrusion processing offers the possibility of processing common beans industrially into highly nutritious and functional products.
28799654	3	24	theme	physico-chemical	423:438	arg1	properties					440:449	physico-chemical properties	423:449	physico-chemical properties of raw and extruded Bishaz, K131, NABE19, Roba1 and RWR2245 common beans	423:522	In this study, physico-chemical properties of raw and extruded Bishaz, K131, NABE19, Roba1 and RWR2245 common beans were determined.
28799654	7	25	with	correlations	1027:1038	arg1	index					1007:1011	water solubility index	990:1011	water solubility index	990:1011	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	7	25	with	correlations	1027:1038	arg1	absorption					1062:1071	water absorption	1056:1071	water absorption	1056:1071	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	7	25	with	correlations	1027:1038	arg1	density					1079:1085	bulk density	1074:1085	bulk density	1074:1085	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	7	25	with	correlations	1027:1038	arg1	viscosities					1099:1109	pasting viscosities	1091:1109	pasting viscosities	1091:1109	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	6	26	theme	peak	854:857	arg1	viscosities					873:883	bulk density, water absorption, peak and breakdown viscosities	822:883	bulk density, water absorption, peak and breakdown viscosities	822:883	Expansion ratio and water solubility decreased, while bulk density, water absorption, peak and breakdown viscosities increased as feed moisture increased.
28799654	1	27	theme	Extrusion	98:106	arg1	processing					108:117	BACKGROUND Extrusion processing	87:117	BACKGROUND Extrusion processing	87:117	BACKGROUND Extrusion processing offers the possibility of processing common beans industrially into highly nutritious and functional products.
28799654	2	28	from	information	256:266	arg1	properties					271:280	properties	271:280	properties of extrudates from different bean varieties	271:324	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	1	29	theme	functional	209:218	arg1	products					220:227	highly nutritious and functional products	187:227	highly nutritious and functional products	187:227	BACKGROUND Extrusion processing offers the possibility of processing common beans industrially into highly nutritious and functional products.
28799654	0	30	theme	Physico-chemical	0:15	arg1	properties					17:26	Physico-chemical properties	0:26	Physico-chemical properties	0:26	Physico-chemical properties and extrusion behaviour of selected common bean varieties.
28799654	8	31	theme	negative	1189:1196	arg1	correlation					1198:1208	negative correlation	1189:1208	negative correlation with peak viscosity, final viscosity and setback	1189:1257	Iron and dietary fibre showed positive correlation while total ash exhibited negative correlation with peak viscosity, final viscosity and setback.
28799654	8	32	theme	positive	1142:1149	arg1	correlation					1151:1161	positive correlation	1142:1161	positive correlation	1142:1161	Iron and dietary fibre showed positive correlation while total ash exhibited negative correlation with peak viscosity, final viscosity and setback.
28799654	2	33	dep	material	357:364	arg1	characteristics					366:380	characteristics	366:380	characteristics	366:380	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	0	34	theme	extrusion	32:40	arg1	behaviour					42:50	extrusion behaviour	32:50	extrusion behaviour	32:50	Physico-chemical properties and extrusion behaviour of selected common bean varieties.
28799654	4	35	theme	extrusion	592:600	arg1	conditions					602:611	extrusion conditions	592:611	extrusion conditions	592:611	The relationships between bean characteristics and extrusion conditions on the extrudate properties were analysed.
28799654	8	36	theme	total	1169:1173	arg1	ash					1175:1177	total ash	1169:1177	total ash	1169:1177	Iron and dietary fibre showed positive correlation while total ash exhibited negative correlation with peak viscosity, final viscosity and setback.
28799654	6	37	theme	absorption	842:851	arg1	viscosities					873:883	bulk density, water absorption, peak and breakdown viscosities	822:883	bulk density, water absorption, peak and breakdown viscosities	822:883	Expansion ratio and water solubility decreased, while bulk density, water absorption, peak and breakdown viscosities increased as feed moisture increased.
28799654	2	38	from	properties	271:280	arg1	varieties					316:324	different bean varieties	301:324	different bean varieties	301:324	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	10	39	theme	physico-chemical	1343:1358	arg1	properties					1360:1369	CONCLUSION Extrudate physico-chemical properties	1322:1369	CONCLUSION Extrudate physico-chemical properties	1322:1369	CONCLUSION Extrudate physico-chemical properties were found to be associated with beans protein, starch, iron, zinc and fibre contents.
28799654	11	40	from	selection	1574:1582	arg1	useful					1564:1569	useful	1564:1569	useful	1564:1569	Therefore, bean chemical composition may serve as an indicator for beans extrusion behaviour and could be useful in selection of beans for extrusion.
28799654	6	41	theme	Expansion	768:776	arg1	ratio					778:782	Expansion ratio	768:782	Expansion ratio	768:782	Expansion ratio and water solubility decreased, while bulk density, water absorption, peak and breakdown viscosities increased as feed moisture increased.
28799654	2	42	with	information	256:266	arg1	material					357:364	raw material characteristics and extrusion conditions	353:405	material	357:364	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	2	42	with	information	256:266	arg1	conditions					396:405	extrusion conditions	386:405	extrusion conditions	386:405	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	7	43	theme	water	990:994	arg1	index					1007:1011	water solubility index	990:1011	water solubility index	990:1011	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	3	44	theme	extruded	462:469	arg1	Bishaz					471:476	Bishaz	471:476	Bishaz	471:476	In this study, physico-chemical properties of raw and extruded Bishaz, K131, NABE19, Roba1 and RWR2245 common beans were determined.
28799654	4	45	theme	bean	567:570	arg1	characteristics					572:586	bean characteristics	567:586	bean characteristics	567:586	The relationships between bean characteristics and extrusion conditions on the extrudate properties were analysed.
28799654	5	46	dep	varied	714:719	arg1	P < 0.05					736:743	P < 0.05	736:743	P < 0.05	736:743	RESULTS Extrudate physico-chemical and pasting properties varied significantly (P < 0.05) among bean varieties.
28799654	6	47	theme	water	836:840	arg1	absorption					842:851	water absorption	836:851	water absorption	836:851	Expansion ratio and water solubility decreased, while bulk density, water absorption, peak and breakdown viscosities increased as feed moisture increased.
28799654	0	48	theme	common	64:69	arg1	varieties					76:84	selected common bean varieties	55:84	selected common bean varieties	55:84	Physico-chemical properties and extrusion behaviour of selected common bean varieties.
28799654	11	49	theme	beans	1587:1591	arg1	selection					1574:1582	selection	1574:1582	selection of beans for extrusion	1574:1605	Therefore, bean chemical composition may serve as an indicator for beans extrusion behaviour and could be useful in selection of beans for extrusion.
28799654	2	50	theme	limited	248:254	arg1	information					256:266	limited information	248:266	limited information on properties of extrudates from different bean varieties	248:324	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	10	51	theme	CONCLUSION	1322:1331	arg1	properties					1360:1369	CONCLUSION Extrudate physico-chemical properties	1322:1369	CONCLUSION Extrudate physico-chemical properties	1322:1369	CONCLUSION Extrudate physico-chemical properties were found to be associated with beans protein, starch, iron, zinc and fibre contents.
28799654	2	52	theme	extrusion	386:394	arg1	conditions					396:405	extrusion conditions	386:405	extrusion conditions	386:405	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	0	53	theme	selected	55:62	arg1	varieties					76:84	selected common bean varieties	55:84	selected common bean varieties	55:84	Physico-chemical properties and extrusion behaviour of selected common bean varieties.
28799654	5	54	theme	bean	752:755	arg1	varieties					757:765	bean varieties	752:765	bean varieties	752:765	RESULTS Extrudate physico-chemical and pasting properties varied significantly (P < 0.05) among bean varieties.
28799654	11	55	from	useful	1564:1569	arg1	selection					1574:1582	selection	1574:1582	selection of beans for extrusion	1574:1605	Therefore, bean chemical composition may serve as an indicator for beans extrusion behaviour and could be useful in selection of beans for extrusion.
28799654	6	56	theme	density	827:833	arg1	viscosities					873:883	bulk density, water absorption, peak and breakdown viscosities	822:883	bulk density, water absorption, peak and breakdown viscosities	822:883	Expansion ratio and water solubility decreased, while bulk density, water absorption, peak and breakdown viscosities increased as feed moisture increased.
28799654	7	57	theme	solubility	996:1005	arg1	index					1007:1011	water solubility index	990:1011	water solubility index	990:1011	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	8	58	theme	peak	1215:1218	arg1	viscosity					1220:1228	peak viscosity	1215:1228	peak viscosity	1215:1228	Iron and dietary fibre showed positive correlation while total ash exhibited negative correlation with peak viscosity, final viscosity and setback.
28799654	9	59	theme	principal	1292:1300	arg1	analysis					1312:1319	principal component analysis	1292:1319	principal component analysis	1292:1319	Similar trends were observed in principal component analysis.
28799654	6	60	theme	bulk	822:825	arg1	density					827:833	bulk density	822:833	bulk density	822:833	Expansion ratio and water solubility decreased, while bulk density, water absorption, peak and breakdown viscosities increased as feed moisture increased.
28799654	1	61	theme	processing	145:154	arg1	beans					163:167	processing common beans	145:167	processing common beans	145:167	BACKGROUND Extrusion processing offers the possibility of processing common beans industrially into highly nutritious and functional products.
28799654	5	62	dep	physico-chemical	674:689	arg1	properties					703:712	properties	703:712	properties	703:712	RESULTS Extrudate physico-chemical and pasting properties varied significantly (P < 0.05) among bean varieties.
28799654	2	63	theme	different	301:309	arg1	varieties					316:324	different bean varieties	301:324	different bean varieties	301:324	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	11	64	theme	beans	1525:1529	arg1	extrusion					1531:1539	beans extrusion behaviour	1525:1549	beans extrusion behaviour	1525:1549	Therefore, bean chemical composition may serve as an indicator for beans extrusion behaviour and could be useful in selection of beans for extrusion.
28799654	2	65	theme	extrudates	285:294	arg1	properties					271:280	properties	271:280	properties of extrudates from different bean varieties	271:324	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	1	66	theme	common	156:161	arg1	beans					163:167	processing common beans	145:167	processing common beans	145:167	BACKGROUND Extrusion processing offers the possibility of processing common beans industrially into highly nutritious and functional products.
28799654	10	67	theme	Extrudate	1333:1341	arg1	properties					1360:1369	CONCLUSION Extrudate physico-chemical properties	1322:1369	CONCLUSION Extrudate physico-chemical properties	1322:1369	CONCLUSION Extrudate physico-chemical properties were found to be associated with beans protein, starch, iron, zinc and fibre contents.
28799654	3	68	theme	RWR2245	503:509	arg1	beans					518:522	RWR2245 common beans	503:522	RWR2245 common beans	503:522	In this study, physico-chemical properties of raw and extruded Bishaz, K131, NABE19, Roba1 and RWR2245 common beans were determined.
28799654	0	69	theme	varieties	76:84	arg1	properties					17:26	Physico-chemical properties	0:26	Physico-chemical properties	0:26	Physico-chemical properties and extrusion behaviour of selected common bean varieties.
28799654	0	69	theme	varieties	76:84	arg1	behaviour					42:50	extrusion behaviour	32:50	extrusion behaviour	32:50	Physico-chemical properties and extrusion behaviour of selected common bean varieties.
28799654	6	70	theme	feed	898:901	arg1	moisture					903:910	feed moisture	898:910	feed moisture	898:910	Expansion ratio and water solubility decreased, while bulk density, water absorption, peak and breakdown viscosities increased as feed moisture increased.
28799654	7	71	theme	bulk	1074:1077	arg1	density					1079:1085	bulk density	1074:1085	bulk density	1074:1085	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	1	72	theme	beans	163:167	arg1	possibility					130:140	the possibility	126:140	the possibility of processing common beans	126:167	BACKGROUND Extrusion processing offers the possibility of processing common beans industrially into highly nutritious and functional products.
28799654	2	73	from	varieties	316:324	arg1	extrudates					285:294	extrudates	285:294	extrudates from different bean varieties	285:324	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	2	73	from	varieties	316:324	arg1	properties					271:280	properties	271:280	properties of extrudates from different bean varieties	271:324	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	0	74	theme	bean	71:74	arg1	varieties					76:84	selected common bean varieties	55:84	selected common bean varieties	55:84	Physico-chemical properties and extrusion behaviour of selected common bean varieties.
28799654	7	75	with	correlation	962:972	arg1	viscosities					1099:1109	pasting viscosities	1091:1109	pasting viscosities	1091:1109	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	7	75	with	correlation	962:972	arg1	absorption					1062:1071	water absorption	1056:1071	water absorption	1056:1071	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	7	75	with	correlation	962:972	arg1	density					1079:1085	bulk density	1074:1085	bulk density	1074:1085	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	7	75	with	correlation	962:972	arg1	index					1007:1011	water solubility index	990:1011	water solubility index	990:1011	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	7	76	theme	water	1056:1060	arg1	absorption					1062:1071	water absorption	1056:1071	water absorption	1056:1071	Protein exhibited significant positive correlation (P < 0.05) with water solubility index, and negative correlations (P < 0.05) with water absorption, bulk density and pasting viscosities.
28799654	10	77	theme	fibre	1442:1446	arg1	contents					1448:1455	fibre contents	1442:1455	fibre contents	1442:1455	CONCLUSION Extrudate physico-chemical properties were found to be associated with beans protein, starch, iron, zinc and fibre contents.
28799654	2	78	from	association	336:346	arg1	properties					271:280	properties	271:280	properties of extrudates from different bean varieties	271:324	However, there is limited information on properties of extrudates from different bean varieties and their association with raw material characteristics and extrusion conditions.
28799654	9	79	located	observed	1280:1287	arg2	trends					1268:1273	Similar trends	1260:1273	Similar trends	1260:1273	Similar trends were observed in principal component analysis.
28799654	9	79	located	observed	1280:1287	arg1	analysis					1312:1319	principal component analysis	1292:1319	principal component analysis	1292:1319	Similar trends were observed in principal component analysis.
27306514	7	0	theme	labeling	1166:1173	arg1	technique					1175:1183	the PMP labeling technique	1158:1183	the PMP labeling technique	1158:1183	Our data show that coexisting carbohydrate isomers can be identified by the PMP labeling technique in conjunction with ion-mobility separation and tandem mass spectrometry.
27306514	5	1	theme	corresponding	933:945	arg1	ions					947:950	the corresponding ions	929:950	the corresponding ions	929:950	To chart the structural variation observed in IMMS, the collision cross sections (CCSs) for the corresponding ions were measured.
27306514	1	2	theme	conventional	270:281	arg1	MS					302:303	MS	302:303	MS	302:303	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	1	2	theme	conventional	270:281	arg1	spectrometry					288:299	conventional mass spectrometry	270:299	conventional mass spectrometry (MS) methods	270:312	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	0	3	theme	PMP	90:92	arg1	labeling					94:101	PMP labeling	90:101	PMP labeling in conjunction with IM-MS/MS	90:130	Identification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS.
27306514	9	4	theme	mixtures	1532:1539	arg1	mixtures					1532:1539	heterogeneous sample mixtures	1511:1539	heterogeneous sample mixtures	1511:1539	This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
27306514	9	4	theme	mixtures	1532:1539	arg1	variety					1500:1506	a variety	1498:1506	a variety	1498:1506	This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
27306514	5	5	located	observed	871:878	arg2	variation					861:869	the structural variation	846:869	the structural variation observed in IMMS	846:886	To chart the structural variation observed in IMMS, the collision cross sections (CCSs) for the corresponding ions were measured.
27306514	5	5	located	observed	871:878	arg1	IMMS					883:886	IMMS	883:886	IMMS	883:886	To chart the structural variation observed in IMMS, the collision cross sections (CCSs) for the corresponding ions were measured.
27306514	2	6	theme	IM	351:352	arg1	strategy					361:368	an ion mobility (IM) shift strategy	334:368	an ion mobility (IM) shift strategy	334:368	In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS.
27306514	1	7	theme	mass	283:286	arg1	MS					302:303	MS	302:303	MS	302:303	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	1	7	theme	mass	283:286	arg1	spectrometry					288:299	conventional mass spectrometry	270:299	conventional mass spectrometry (MS) methods	270:312	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	8	8	theme	water-soluble	1409:1421	arg1	extract					1431:1437	water-soluble ginseng extract	1409:1437	water-soluble ginseng extract	1409:1437	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	3	9	theme	PMP	607:609	arg1	derivatization					612:625	1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	575:625	1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	575:625	Higher separation between structural isomers was achieved using 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization in comparison with phenylhydrazine (PHN) derivatization.
27306514	1	10	theme	spectrometry	288:299	arg1	methods					306:312	conventional mass spectrometry (MS) methods	270:312	conventional mass spectrometry (MS) methods	270:312	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	3	11	with	comparison	630:639	arg1	derivatization					668:681	phenylhydrazine (PHN) derivatization	646:681	phenylhydrazine (PHN) derivatization	646:681	Higher separation between structural isomers was achieved using 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization in comparison with phenylhydrazine (PHN) derivatization.
27306514	7	12	theme	PMP	1162:1164	arg1	technique					1175:1183	the PMP labeling technique	1158:1183	the PMP labeling technique	1158:1183	Our data show that coexisting carbohydrate isomers can be identified by the PMP labeling technique in conjunction with ion-mobility separation and tandem mass spectrometry.
27306514	3	13	theme	1-phenyl-3-methyl-5-pyrazolone	575:604	arg1	derivatization					612:625	1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	575:625	1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	575:625	Higher separation between structural isomers was achieved using 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization in comparison with phenylhydrazine (PHN) derivatization.
27306514	4	14	theme	derivatives	744:754	arg1	fragmentation					723:735	pre-IM fragmentation	716:735	pre-IM fragmentation of PMP derivatives	716:754	Furthermore, the combination of pre-IM fragmentation of PMP derivatives provided sufficient resolution to separate the isomers not resolved in the IMMS.
27306514	8	15	theme	sample	1356:1361	arg1	sample					1356:1361	sample	1356:1361	sample	1356:1361	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	8	15	theme	sample	1356:1361	arg1	amounts					1345:1351	only small amounts	1334:1351	only small amounts of sample	1334:1361	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	9	16	contain	have	1552:1555	arg1	mixtures					1532:1539	heterogeneous sample mixtures	1511:1539	heterogeneous sample mixtures	1511:1539	This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
27306514	9	16	contain	have	1552:1555	arg2	impact					1570:1575	an important impact	1557:1575	an important impact	1557:1575	This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
27306514	9	16	contain	have	1552:1555	arg1	variety					1500:1506	a variety	1498:1506	a variety	1498:1506	This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
27306514	8	17	theme	extract	1431:1437	arg1	analysis					1397:1404	the successful analysis	1382:1404	the successful analysis of water-soluble ginseng extract	1382:1437	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	9	18	theme	sample	1525:1530	arg1	mixtures					1532:1539	heterogeneous sample mixtures	1511:1539	heterogeneous sample mixtures	1511:1539	This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
27306514	8	19	theme	effective	1303:1311	arg1	method					1313:1318	this rapid and effective method	1288:1318	this rapid and effective method that requires only small amounts of sample	1288:1361	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	5	20	theme	structural	850:859	arg1	variation					861:869	the structural variation	846:869	the structural variation observed in IMMS	846:886	To chart the structural variation observed in IMMS, the collision cross sections (CCSs) for the corresponding ions were measured.
27306514	2	21	theme	isomers	491:497	arg1	identification					412:425	the identification	408:425	the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS	408:508	In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS.
27306514	8	22	theme	ginseng	1423:1429	arg1	extract					1431:1437	water-soluble ginseng extract	1409:1437	water-soluble ginseng extract	1409:1437	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	9	23	theme	heterogeneous	1511:1523	arg1	mixtures					1532:1539	heterogeneous sample mixtures	1511:1539	heterogeneous sample mixtures	1511:1539	This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
27306514	2	24	theme	related	451:457	arg1	disaccharide					459:470	structurally closely related disaccharide	430:470	structurally closely related disaccharide	430:470	In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS.
27306514	7	25	theme	tandem	1233:1238	arg1	spectrometry					1245:1256	tandem mass spectrometry	1233:1256	tandem mass spectrometry	1233:1256	Our data show that coexisting carbohydrate isomers can be identified by the PMP labeling technique in conjunction with ion-mobility separation and tandem mass spectrometry.
27306514	4	26	theme	fragmentation	723:735	arg1	combination					701:711	the combination	697:711	the combination of pre-IM fragmentation of PMP derivatives	697:754	Furthermore, the combination of pre-IM fragmentation of PMP derivatives provided sufficient resolution to separate the isomers not resolved in the IMMS.
27306514	7	27	theme	mass	1240:1243	arg1	spectrometry					1245:1256	tandem mass spectrometry	1233:1256	tandem mass spectrometry	1233:1256	Our data show that coexisting carbohydrate isomers can be identified by the PMP labeling technique in conjunction with ion-mobility separation and tandem mass spectrometry.
27306514	7	28	theme	ion-mobility	1205:1216	arg1	separation					1218:1227	ion-mobility separation	1205:1227	ion-mobility separation	1205:1227	Our data show that coexisting carbohydrate isomers can be identified by the PMP labeling technique in conjunction with ion-mobility separation and tandem mass spectrometry.
27306514	0	29	with	conjunction	106:116	arg1	IM-MS/MS					123:130	IM-MS/MS	123:130	IM-MS/MS	123:130	Identification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS.
27306514	4	30	theme	pre-IM	716:721	arg1	fragmentation					723:735	pre-IM fragmentation	716:735	pre-IM fragmentation of PMP derivatives	716:754	Furthermore, the combination of pre-IM fragmentation of PMP derivatives provided sufficient resolution to separate the isomers not resolved in the IMMS.
27306514	8	31	theme	practical	1263:1271	arg1	application					1273:1283	The practical application	1259:1283	The practical application of this rapid and effective method that requires only small amounts of sample	1259:1361	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	7	32	theme	carbohydrate	1116:1127	arg1	isomers					1129:1135	coexisting carbohydrate isomers	1105:1135	coexisting carbohydrate isomers	1105:1135	Our data show that coexisting carbohydrate isomers can be identified by the PMP labeling technique in conjunction with ion-mobility separation and tandem mass spectrometry.
27306514	8	33	theme	successful	1386:1395	arg1	analysis					1397:1404	the successful analysis	1382:1404	the successful analysis of water-soluble ginseng extract	1382:1437	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	6	34	theme	monosaccharide	1007:1020	arg1	isomers					1022:1028	nine disaccharide and three monosaccharide isomers	979:1028	isomers	1022:1028	We analyzed nine disaccharide and three monosaccharide isomers that differ in composition, linkages, or configuration.
27306514	0	35	from	labeling	94:101	arg1	conjunction					106:116	conjunction	106:116	conjunction with IM-MS/MS	106:130	Identification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS.
27306514	1	36	theme	unambiguous	179:189	arg1	information					191:201	unambiguous information	179:201	unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods	179:312	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	9	37	theme	glycomics	1593:1601	arg1	field					1584:1588	the field	1580:1588	the field of glycomics	1580:1601	This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
27306514	4	38	theme	PMP	740:742	arg1	derivatives					744:754	PMP derivatives	740:754	PMP derivatives	740:754	Furthermore, the combination of pre-IM fragmentation of PMP derivatives provided sufficient resolution to separate the isomers not resolved in the IMMS.
27306514	0	39	theme	monosaccharide	47:60	arg1	isomers					79:85	structurally closely related monosaccharide and disaccharide isomers	18:85	structurally closely related monosaccharide and disaccharide isomers	18:85	Identification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS.
27306514	3	40	theme	Higher	511:516	arg1	separation					518:527	Higher separation	511:527	Higher separation between structural isomers	511:554	Higher separation between structural isomers was achieved using 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization in comparison with phenylhydrazine (PHN) derivatization.
27306514	9	41	theme	important	1560:1568	arg1	impact					1570:1575	an important impact	1557:1575	an important impact	1557:1575	This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
27306514	8	42	theme	method	1313:1318	arg1	application					1273:1283	The practical application	1259:1283	The practical application of this rapid and effective method that requires only small amounts of sample	1259:1361	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	5	43	theme	collision	893:901	arg1	CCSs					919:922	CCSs	919:922	CCSs	919:922	To chart the structural variation observed in IMMS, the collision cross sections (CCSs) for the corresponding ions were measured.
27306514	5	43	theme	collision	893:901	arg1	sections					909:916	the collision cross sections	889:916	the collision cross sections (CCSs) for the corresponding ions	889:950	To chart the structural variation observed in IMMS, the collision cross sections (CCSs) for the corresponding ions were measured.
27306514	2	44	theme	monosaccharide	476:489	arg1	isomers					491:497	monosaccharide isomers	476:497	monosaccharide isomers using IMMS	476:508	In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS.
27306514	0	45	theme	related	39:45	arg1	isomers					79:85	structurally closely related monosaccharide and disaccharide isomers	18:85	structurally closely related monosaccharide and disaccharide isomers	18:85	Identification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS.
27306514	3	46	theme	phenylhydrazine	646:660	arg1	derivatization					668:681	phenylhydrazine (PHN) derivatization	646:681	phenylhydrazine (PHN) derivatization	646:681	Higher separation between structural isomers was achieved using 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization in comparison with phenylhydrazine (PHN) derivatization.
27306514	1	47	theme	position	227:234	arg1	isomers					236:242	position isomers	227:242	position isomers	227:242	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	0	48	theme	disaccharide	66:77	arg1	isomers					79:85	structurally closely related monosaccharide and disaccharide isomers	18:85	structurally closely related monosaccharide and disaccharide isomers	18:85	Identification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS.
27306514	7	49	theme	coexisting	1105:1114	arg1	isomers					1129:1135	coexisting carbohydrate isomers	1105:1135	coexisting carbohydrate isomers	1105:1135	Our data show that coexisting carbohydrate isomers can be identified by the PMP labeling technique in conjunction with ion-mobility separation and tandem mass spectrometry.
27306514	2	50	theme	disaccharide	459:470	arg1	identification					412:425	the identification	408:425	the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS	408:508	In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS.
27306514	7	51	with	conjunction	1188:1198	arg1	spectrometry					1245:1256	tandem mass spectrometry	1233:1256	tandem mass spectrometry	1233:1256	Our data show that coexisting carbohydrate isomers can be identified by the PMP labeling technique in conjunction with ion-mobility separation and tandem mass spectrometry.
27306514	7	51	with	conjunction	1188:1198	arg1	separation					1218:1227	ion-mobility separation	1205:1227	ion-mobility separation	1205:1227	Our data show that coexisting carbohydrate isomers can be identified by the PMP labeling technique in conjunction with ion-mobility separation and tandem mass spectrometry.
27306514	2	52	theme	mobility	341:348	arg1	strategy					361:368	an ion mobility (IM) shift strategy	334:368	an ion mobility (IM) shift strategy	334:368	In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS.
27306514	2	53	theme	ion	337:339	arg1	strategy					361:368	an ion mobility (IM) shift strategy	334:368	an ion mobility (IM) shift strategy	334:368	In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS.
27306514	8	54	theme	small	1339:1343	arg1	sample					1356:1361	sample	1356:1361	sample	1356:1361	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	8	54	theme	small	1339:1343	arg1	amounts					1345:1351	only small amounts	1334:1351	only small amounts of sample	1334:1361	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	8	55	theme	rapid	1293:1297	arg1	method					1313:1318	this rapid and effective method	1288:1318	this rapid and effective method that requires only small amounts of sample	1288:1361	The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract.
27306514	5	56	theme	cross	903:907	arg1	CCSs					919:922	CCSs	919:922	CCSs	919:922	To chart the structural variation observed in IMMS, the collision cross sections (CCSs) for the corresponding ions were measured.
27306514	5	56	theme	cross	903:907	arg1	sections					909:916	the collision cross sections	889:916	the collision cross sections (CCSs) for the corresponding ions	889:950	To chart the structural variation observed in IMMS, the collision cross sections (CCSs) for the corresponding ions were measured.
27306514	2	57	from	confidence	394:403	arg1	identification					412:425	the identification	408:425	the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS	408:508	In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS.
27306514	3	58	theme	structural	537:546	arg1	isomers					548:554	structural isomers	537:554	structural isomers	537:554	Higher separation between structural isomers was achieved using 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization in comparison with phenylhydrazine (PHN) derivatization.
27306514	1	59	theme	oligosaccharides	247:262	arg1	anomer					206:211	anomer	206:211	anomer	206:211	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	1	59	theme	oligosaccharides	247:262	arg1	isomers					236:242	position isomers	227:242	position isomers	227:242	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	1	59	theme	oligosaccharides	247:262	arg1	linkage					214:220	linkage	214:220	linkage	214:220	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	0	60	theme	isomers	79:85	arg1	Identification					0:13	Identification	0:13	Identification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS.	0:131	Identification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS.
27306514	9	61	theme	method	1480:1485	arg1	potential					1462:1470	the potential	1458:1470	the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics	1458:1601	This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
27306514	1	62	from	information	191:201	arg1	anomer					206:211	anomer	206:211	anomer	206:211	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	1	62	from	information	191:201	arg1	isomers					236:242	position isomers	227:242	position isomers	227:242	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	1	62	from	information	191:201	arg1	linkage					214:220	linkage	214:220	linkage	214:220	It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods.
27306514	2	63	theme	shift	355:359	arg1	strategy					361:368	an ion mobility (IM) shift strategy	334:368	an ion mobility (IM) shift strategy	334:368	In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS.
27306514	4	64	theme	sufficient	765:774	arg1	resolution					776:785	sufficient resolution	765:785	sufficient resolution	765:785	Furthermore, the combination of pre-IM fragmentation of PMP derivatives provided sufficient resolution to separate the isomers not resolved in the IMMS.
25839135	0	0	theme	globo	120:124	arg1	H					126:126	globo H	120:126	globo H	120:126	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.
25839135	5	1	theme	GNB	831:833	arg1	release					811:817	enzymatic release	801:817	enzymatic release of β-linked GNB from natural substrates	801:857	This is the first report describing enzymatic release of β-linked GNB from natural substrates.
25839135	1	2	theme	novel	159:163	arg1	specificities					175:187	the novel substrate specificities	155:187	the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids	155:324	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	1	3	theme	substrate	165:173	arg1	specificities					175:187	the novel substrate specificities	155:187	the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids	155:324	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	1	4	theme	sugar	265:269	arg1	chains					271:276	the sugar chains	261:276	the sugar chains of globo- and ganglio-series glycosphingolipids	261:324	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	6	5	theme	sugar	1022:1026	arg1	chains					1028:1033	sugar chains	1022:1033	sugar chains of glycosphingolipids	1022:1055	These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
25839135	0	6	theme	H	126:126	arg1	oligosaccharides					100:115	β-linked galacto-N-biose-containing oligosaccharides	64:115	β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1	64:140	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.
25839135	3	7	theme	Gb5	552:554	arg1	pentasaccharide					556:570	Gb5 pentasaccharide	552:570	Gb5 pentasaccharide	552:570	longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively.
25839135	2	8	theme	hydrolase	374:382	arg1	family					384:389	the glycoside hydrolase family	360:389	the glycoside hydrolase family	360:389	LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp.
25839135	5	9	theme	enzymatic	801:809	arg1	release					811:817	enzymatic release	801:817	enzymatic release of β-linked GNB from natural substrates	801:857	This is the first report describing enzymatic release of β-linked GNB from natural substrates.
25839135	6	10	theme	chains	1028:1033	arg1	functions					1009:1017	the functions	1005:1017	the functions of sugar chains of glycosphingolipids	1005:1055	These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
25839135	0	11	theme	Gb5	129:131	arg1	oligosaccharides					100:115	β-linked galacto-N-biose-containing oligosaccharides	64:115	β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1	64:140	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.
25839135	0	12	link	β-linked	64:71	arg1	oligosaccharides					100:115	β-linked galacto-N-biose-containing oligosaccharides	64:115	β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1	64:140	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.
25839135	2	13	theme	Bifidobacterium	406:420	arg1	subsp					429:433	Bifidobacterium longum subsp	406:433	Bifidobacterium longum subsp	406:433	LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp.
25839135	0	14	theme	substrate	6:14	arg1	specificities					16:28	Novel substrate specificities	0:28	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.	0:141	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.
25839135	3	15	theme	type-4	525:530	arg1	trisaccharide					532:544	a type-4 trisaccharide	523:544	a type-4 trisaccharide	523:544	longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively.
25839135	3	15	theme	type-4	525:530	arg1	GNB					518:520	2'-fucosyl GNB	507:520	2'-fucosyl GNB (a type-4 trisaccharide)	507:545	longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively.
25839135	3	16	theme	globo	576:580	arg1	H					582:582	globo H	576:582	globo H	576:582	longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively.
25839135	0	17	theme	Novel	0:4	arg1	specificities					16:28	Novel substrate specificities	0:28	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.	0:141	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.
25839135	1	18	theme	globo-	281:286	arg1	glycosphingolipids					307:324	globo- and ganglio-series glycosphingolipids	281:324	globo- and ganglio-series glycosphingolipids	281:324	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	6	19	theme	glycosphingolipids	1038:1055	arg1	chains					1028:1033	sugar chains	1022:1033	sugar chains of glycosphingolipids	1022:1055	These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
25839135	0	20	theme	GA1	138:140	arg1	oligosaccharides					100:115	β-linked galacto-N-biose-containing oligosaccharides	64:115	β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1	64:140	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.
25839135	4	21	theme	GA1	743:745	arg1	oligosaccharides					747:762	GA1 oligosaccharides	743:762	GA1 oligosaccharides	743:762	LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, was shown to release GNB from Gb5 and GA1 oligosaccharides.
25839135	1	22	theme	evolved	210:216	arg1	lacto-N-biosidases					218:235	two independently evolved lacto-N-biosidases	192:235	two independently evolved lacto-N-biosidases (LnbX and LnbB)	192:251	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	1	22	theme	evolved	210:216	arg1	LnbB					247:250	LnbB	247:250	LnbB	247:250	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	1	22	theme	evolved	210:216	arg1	LnbX					238:241	LnbX	238:241	LnbX	238:241	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	5	23	theme	β-linked	822:829	arg1	GNB					831:833	β-linked GNB	822:833	β-linked GNB	822:833	This is the first report describing enzymatic release of β-linked GNB from natural substrates.
25839135	1	24	theme	ganglio-series	292:305	arg1	glycosphingolipids					307:324	globo- and ganglio-series glycosphingolipids	281:324	globo- and ganglio-series glycosphingolipids	281:324	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	2	25	theme	glycoside	364:372	arg1	family					384:389	the glycoside hydrolase family	360:389	the glycoside hydrolase family	360:389	LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp.
25839135	1	26	theme	lacto-N-biosidases	218:235	arg1	specificities					175:187	the novel substrate specificities	155:187	the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids	155:324	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	0	27	theme	lacto-N-biosidases	37:54	arg1	specificities					16:28	Novel substrate specificities	0:28	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.	0:141	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.
25839135	1	28	theme	glycosphingolipids	307:324	arg1	chains					271:276	the sugar chains	261:276	the sugar chains of globo- and ganglio-series glycosphingolipids	261:324	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	5	29	theme	natural	840:846	arg1	substrates					848:857	natural substrates	840:857	natural substrates	840:857	This is the first report describing enzymatic release of β-linked GNB from natural substrates.
25839135	4	30	theme	hydrolase	646:654	arg1	family					656:661	the glycoside hydrolase family 20	632:664	the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum	632:702	LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, was shown to release GNB from Gb5 and GA1 oligosaccharides.
25839135	5	31	theme	first	777:781	arg1	report					783:788	the first report	773:788	the first report describing enzymatic release of β-linked GNB from natural substrates	773:857	This is the first report describing enzymatic release of β-linked GNB from natural substrates.
25839135	5	31	theme	first	777:781	arg1	This					765:768	This	765:768	This	765:768	This is the first report describing enzymatic release of β-linked GNB from natural substrates.
25839135	6	32	theme	gut	947:949	arg1	ecosystem					951:959	the gut ecosystem	943:959	the gut ecosystem	943:959	These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
25839135	6	33	theme	new	979:981	arg1	tools					983:987	new tools	979:987	new tools for elucidating the functions of sugar chains of glycosphingolipids	979:1055	These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
25839135	6	33	theme	new	979:981	arg1	activities					873:882	These unique activities	860:882	These unique activities	860:882	These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
25839135	4	34	attach	isolated	666:673	arg2	family					656:661	the glycoside hydrolase family 20	632:664	the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum	632:702	LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, was shown to release GNB from Gb5 and GA1 oligosaccharides.
25839135	4	34	attach	isolated	666:673	arg1	bifidum					696:702	Bifidobacterium bifidum	680:702	Bifidobacterium bifidum	680:702	LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, was shown to release GNB from Gb5 and GA1 oligosaccharides.
25839135	4	35	theme	glycoside	636:644	arg1	family					656:661	the glycoside hydrolase family 20	632:664	the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum	632:702	LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, was shown to release GNB from Gb5 and GA1 oligosaccharides.
25839135	0	36	theme	β-linked	64:71	arg1	oligosaccharides					100:115	β-linked galacto-N-biose-containing oligosaccharides	64:115	β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1	64:140	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.
25839135	5	37	from	substrates	848:857	arg1	release					811:817	enzymatic release	801:817	enzymatic release of β-linked GNB from natural substrates	801:857	This is the first report describing enzymatic release of β-linked GNB from natural substrates.
25839135	6	38	from	composition	928:938	arg1	ecosystem					951:959	the gut ecosystem	943:959	the gut ecosystem	943:959	These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
25839135	3	39	theme	2'-fucosyl	507:516	arg1	trisaccharide					532:544	a type-4 trisaccharide	523:544	a type-4 trisaccharide	523:544	longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively.
25839135	3	39	theme	2'-fucosyl	507:516	arg1	GNB					518:520	2'-fucosyl GNB	507:520	2'-fucosyl GNB (a type-4 trisaccharide)	507:545	longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively.
25839135	2	40	dep	Bifidobacterium	406:420	arg1	longum					422:427	longum	422:427	longum	422:427	LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp.
25839135	5	41	link	β-linked	822:829	arg1	GNB					831:833	β-linked GNB	822:833	β-linked GNB	822:833	This is the first report describing enzymatic release of β-linked GNB from natural substrates.
25839135	2	42	theme	non-classified	335:348	arg1	LnbX					327:330	LnbX	327:330	LnbX	327:330	LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp.
25839135	2	42	theme	non-classified	335:348	arg1	member					350:355	a non-classified member	333:355	a non-classified member of the glycoside hydrolase family	333:389	LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp.
25839135	6	43	theme	unique	866:871	arg1	tools					983:987	new tools	979:987	new tools for elucidating the functions of sugar chains of glycosphingolipids	979:1055	These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
25839135	6	43	theme	unique	866:871	arg1	activities					873:882	These unique activities	860:882	These unique activities	860:882	These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
25839135	3	44	dep	GNB	483:485	arg1	Galβ1-3GalNAc					488:500	Galβ1-3GalNAc	488:500	GNB: Galβ1-3GalNAc	483:500	longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively.
25839135	3	45	dep	pentasaccharide	556:570	arg1	hexasaccharide					584:597	hexasaccharide	584:597	hexasaccharide	584:597	longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively.
25839135	3	46	theme	a	523:523	arg1	trisaccharide					532:544	a type-4 trisaccharide	523:544	a type-4 trisaccharide	523:544	longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively.
25839135	3	46	theme	a	523:523	arg1	GNB					518:520	2'-fucosyl GNB	507:520	2'-fucosyl GNB (a type-4 trisaccharide)	507:545	longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively.
25839135	4	47	theme	family	656:661	arg1	LnbB					614:617	LnbB	614:617	LnbB	614:617	LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, was shown to release GNB from Gb5 and GA1 oligosaccharides.
25839135	4	47	theme	family	656:661	arg1	member					622:627	a member	620:627	a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum	620:702	LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, was shown to release GNB from Gb5 and GA1 oligosaccharides.
25839135	0	48	theme	galacto-N-biose-containing	73:98	arg1	oligosaccharides					100:115	β-linked galacto-N-biose-containing oligosaccharides	64:115	β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1	64:140	Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.
25839135	6	49	theme	microbial	918:926	arg1	composition					928:938	the microbial composition	914:938	the microbial composition in the gut ecosystem	914:959	These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
25839135	1	50	dep	lacto-N-biosidases	218:235	arg1	lacto-N-biosidases					218:235	two independently evolved lacto-N-biosidases	192:235	two independently evolved lacto-N-biosidases (LnbX and LnbB)	192:251	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	1	50	dep	lacto-N-biosidases	218:235	arg1	LnbB					247:250	LnbB	247:250	LnbB	247:250	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	1	50	dep	lacto-N-biosidases	218:235	arg1	LnbX					238:241	LnbX	238:241	LnbX	238:241	We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids.
25839135	2	51	theme	family	384:389	arg1	LnbX					327:330	LnbX	327:330	LnbX	327:330	LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp.
25839135	2	51	theme	family	384:389	arg1	member					350:355	a non-classified member	333:355	a non-classified member of the glycoside hydrolase family	333:389	LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp.
25265162	3	0	theme	Janus	797:801	arg1	microparticles					803:816	Janus microparticles	797:816	Janus microparticles	797:816	We demonstrate here a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles.
25265162	4	1	theme	simple	1009:1014	arg1	evaporation					1016:1026	simple evaporation	1009:1026	simple evaporation of solvent	1009:1037	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	2	2	theme	electronic	535:544	arg1	paper					546:550	"twisting ball type" electronic paper	514:550	"twisting ball type" electronic paper	514:550	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	3	3	theme	polymer	779:785	arg1	phases					787:792	distinct polymer phases	770:792	distinct polymer phases in Janus microparticles	770:816	We demonstrate here a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles.
25265162	2	4	theme	good	482:485	arg1	microparticles					458:471	Janus microparticles	452:471	Janus microparticles	452:471	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	2	4	theme	good	482:485	arg1	candidates					487:496	good candidates	482:496	good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices	482:626	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	1	5	theme	Janus	222:226	arg1	microparticles					228:241	Janus microparticles	222:241	Janus microparticles	222:241	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	3	6	theme	different	726:734	arg1	nanoparticles					751:763	different such inorganic nanoparticles	726:763	different such inorganic nanoparticles	726:763	We demonstrate here a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles.
25265162	0	7	theme	amphiphilic	138:148	arg1	polymers					150:157	mussel-inspired amphiphilic polymers	122:157	mussel-inspired amphiphilic polymers	122:157	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles encapsulated in mussel-inspired amphiphilic polymers.
25265162	5	8	theme	magnetic	1182:1189	arg1	fields					1191:1196	applied magnetic fields	1174:1196	applied magnetic fields	1174:1196	These Janus microparticles were observed to rotate quickly in response to applied magnetic fields.
25265162	0	9	theme	mussel-inspired	122:136	arg1	polymers					150:157	mussel-inspired amphiphilic polymers	122:157	mussel-inspired amphiphilic polymers	122:157	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles encapsulated in mussel-inspired amphiphilic polymers.
25265162	1	10	theme	external	414:421	arg1	fields					444:449	external electric or magnetic fields	414:449	external electric or magnetic fields	414:449	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	1	11	theme	much	322:325	arg1	interest					327:334	much interest	322:334	much interest	322:334	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	1	12	theme	electric	423:430	arg1	fields					444:449	external electric or magnetic fields	414:449	external electric or magnetic fields	414:449	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	0	13	contain	containing	66:75	arg2	Fe2O3					86:90	Fe2O3	86:90	Fe2O3	86:90	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles encapsulated in mussel-inspired amphiphilic polymers.
25265162	0	13	contain	containing	66:75	arg2	TiO2					77:80	TiO2	77:80	TiO2	77:80	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles encapsulated in mussel-inspired amphiphilic polymers.
25265162	0	13	contain	containing	66:75	arg1	microparticles					51:64	Double-phase-functionalized magnetic Janus polymer microparticles	0:64	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles	0:104	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles encapsulated in mussel-inspired amphiphilic polymers.
25265162	4	14	theme	different	884:892	arg1	copolymers					922:931	two different mussel-inspired amphiphilic copolymers	880:931	two different mussel-inspired amphiphilic copolymers	880:931	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	4	15	theme	Janus	971:975	arg1	microparticles					977:990	then organic-inorganic composite Janus microparticles	938:990	then organic-inorganic composite Janus microparticles	938:990	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	2	16	theme	Janus	452:456	arg1	microparticles					458:471	Janus microparticles	452:471	Janus microparticles	452:471	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	2	16	theme	Janus	452:456	arg1	candidates					487:496	good candidates	482:496	good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices	482:626	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	0	17	theme	magnetic	28:35	arg1	microparticles					51:64	Double-phase-functionalized magnetic Janus polymer microparticles	0:64	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles	0:104	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles encapsulated in mussel-inspired amphiphilic polymers.
25265162	1	18	theme	magnetic	435:442	arg1	fields					444:449	external electric or magnetic fields	414:449	external electric or magnetic fields	414:449	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	3	19	dep	strategy	661:668	arg1	introduce					716:724	introduce	716:724	to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles	713:816	We demonstrate here a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles.
25265162	3	19	dep	strategy	661:668	arg1	encapsulate					673:683	encapsulate	673:683	to encapsulate inorganic nanoparticles	670:707	We demonstrate here a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles.
25265162	0	20	theme	Double-phase-functionalized	0:26	arg1	microparticles					51:64	Double-phase-functionalized magnetic Janus polymer microparticles	0:64	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles	0:104	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles encapsulated in mussel-inspired amphiphilic polymers.
25265162	4	21	theme	TiO2	819:822	arg1	nanoparticles					834:846	TiO2 and Fe2O3 nanoparticles	819:846	TiO2 and Fe2O3 nanoparticles	819:846	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	4	22	theme	Fe2O3	828:832	arg1	nanoparticles					834:846	TiO2 and Fe2O3 nanoparticles	819:846	TiO2 and Fe2O3 nanoparticles	819:846	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	0	23	theme	polymer	43:49	arg1	microparticles					51:64	Double-phase-functionalized magnetic Janus polymer microparticles	0:64	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles	0:104	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles encapsulated in mussel-inspired amphiphilic polymers.
25265162	4	24	theme	organic-inorganic	943:959	arg1	microparticles					977:990	then organic-inorganic composite Janus microparticles	938:990	then organic-inorganic composite Janus microparticles	938:990	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	0	25	theme	Janus	37:41	arg1	microparticles					51:64	Double-phase-functionalized magnetic Janus polymer microparticles	0:64	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles	0:104	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles encapsulated in mussel-inspired amphiphilic polymers.
25265162	3	26	from	phases	787:792	arg1	microparticles					803:816	Janus microparticles	797:816	Janus microparticles	797:816	We demonstrate here a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles.
25265162	2	27	theme	display	612:618	arg1	devices					620:626	next-generation flexible display devices	587:626	next-generation flexible display devices	587:626	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	1	28	contain	have	250:253	arg1	materials					202:210	anisotropic colloidal polymeric materials	170:210	anisotropic colloidal polymeric materials	170:210	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	1	28	contain	have	250:253	arg1	microparticles					228:241	Janus microparticles	222:241	Janus microparticles	222:241	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	1	28	contain	have	250:253	arg2	aspects					268:274	two distinct aspects	255:274	two distinct aspects	255:274	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	5	29	theme	Janus	1106:1110	arg1	microparticles					1112:1125	These Janus microparticles	1100:1125	These Janus microparticles	1100:1125	These Janus microparticles were observed to rotate quickly in response to applied magnetic fields.
25265162	4	30	contain	containing	1059:1068	arg2	nanoparticle					1086:1097	nanoparticle	1086:1097	nanoparticle	1086:1097	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	4	30	contain	containing	1059:1068	arg1	dispersion					1048:1057	the dispersion	1044:1057	the dispersion containing the polymer and nanoparticle	1044:1097	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	4	30	contain	containing	1059:1068	arg2	polymer					1074:1080	the polymer	1070:1080	the polymer	1070:1080	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	1	31	theme	distinct	259:266	arg1	aspects					268:274	two distinct aspects	255:274	two distinct aspects	255:274	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	2	32	theme	"	533:533	arg1	paper					546:550	"twisting ball type" electronic paper	514:550	"twisting ball type" electronic paper	514:550	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	2	33	theme	flexible	603:610	arg1	devices					620:626	next-generation flexible display devices	587:626	next-generation flexible display devices	587:626	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	3	34	theme	such	736:739	arg1	nanoparticles					751:763	different such inorganic nanoparticles	726:763	different such inorganic nanoparticles	726:763	We demonstrate here a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles.
25265162	2	35	theme	type	529:532	arg1	paper					546:550	"twisting ball type" electronic paper	514:550	"twisting ball type" electronic paper	514:550	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	1	36	theme	anisotropic	349:359	arg1	alignment					361:369	their anisotropic alignment	343:369	their anisotropic alignment	343:369	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	2	37	theme	next-generation	587:601	arg1	devices					620:626	next-generation flexible display devices	587:626	next-generation flexible display devices	587:626	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	0	38	dep	TiO2	77:80	arg1	nanoparticles					92:104	nanoparticles	92:104	nanoparticles	92:104	Double-phase-functionalized magnetic Janus polymer microparticles containing TiO2 and Fe2O3 nanoparticles encapsulated in mussel-inspired amphiphilic polymers.
25265162	2	39	theme	ball	524:527	arg1	paper					546:550	"twisting ball type" electronic paper	514:550	"twisting ball type" electronic paper	514:550	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	4	40	theme	composite	961:969	arg1	microparticles					977:990	then organic-inorganic composite Janus microparticles	938:990	then organic-inorganic composite Janus microparticles	938:990	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	2	41	theme	twisting	515:522	arg1	paper					546:550	"twisting ball type" electronic paper	514:550	"twisting ball type" electronic paper	514:550	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	4	42	theme	solvent	1031:1037	arg1	evaporation					1016:1026	simple evaporation	1009:1026	simple evaporation of solvent	1009:1037	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	3	43	theme	universal	651:659	arg1	strategy					661:668	a universal strategy	649:668	a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles	649:816	We demonstrate here a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles.
25265162	1	44	theme	rotational	375:384	arg1	orientation					386:396	rotational orientation	375:396	rotational orientation	375:396	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	3	45	theme	distinct	770:777	arg1	phases					787:792	distinct polymer phases	770:792	distinct polymer phases in Janus microparticles	770:816	We demonstrate here a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles.
25265162	4	46	theme	amphiphilic	910:920	arg1	copolymers					922:931	two different mussel-inspired amphiphilic copolymers	880:931	two different mussel-inspired amphiphilic copolymers	880:931	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	5	47	theme	applied	1174:1180	arg1	fields					1191:1196	applied magnetic fields	1174:1196	applied magnetic fields	1174:1196	These Janus microparticles were observed to rotate quickly in response to applied magnetic fields.
25265162	1	48	theme	anisotropic	170:180	arg1	materials					202:210	anisotropic colloidal polymeric materials	170:210	anisotropic colloidal polymeric materials	170:210	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	1	48	theme	anisotropic	170:180	arg1	microparticles					228:241	Janus microparticles	222:241	Janus microparticles	222:241	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	3	49	theme	inorganic	741:749	arg1	nanoparticles					751:763	different such inorganic nanoparticles	726:763	different such inorganic nanoparticles	726:763	We demonstrate here a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles.
25265162	4	50	theme	mussel-inspired	894:908	arg1	copolymers					922:931	two different mussel-inspired amphiphilic copolymers	880:931	two different mussel-inspired amphiphilic copolymers	880:931	TiO2 and Fe2O3 nanoparticles were separately encapsulated in two different mussel-inspired amphiphilic copolymers, and then organic-inorganic composite Janus microparticles were prepared by simple evaporation of solvent from the dispersion containing the polymer and nanoparticle.
25265162	1	51	theme	colloidal	182:190	arg1	materials					202:210	anisotropic colloidal polymeric materials	170:210	anisotropic colloidal polymeric materials	170:210	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	1	51	theme	colloidal	182:190	arg1	microparticles					228:241	Janus microparticles	222:241	Janus microparticles	222:241	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	1	52	theme	polymeric	192:200	arg1	materials					202:210	anisotropic colloidal polymeric materials	170:210	anisotropic colloidal polymeric materials	170:210	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	1	52	theme	polymeric	192:200	arg1	microparticles					228:241	Janus microparticles	222:241	Janus microparticles	222:241	Recently, anisotropic colloidal polymeric materials including Janus microparticles, which have two distinct aspects on their surfaces or interiors, have garnered much interest due to their anisotropic alignment and rotational orientation with respect to external electric or magnetic fields.
25265162	2	53	from	pigments	502:509	arg1	paper					546:550	"twisting ball type" electronic paper	514:550	"twisting ball type" electronic paper	514:550	Janus microparticles are also good candidates for pigments in "twisting ball type" electronic paper, which is considered promising for next-generation flexible display devices.
25265162	3	54	theme	inorganic	685:693	arg1	nanoparticles					695:707	inorganic nanoparticles	685:707	inorganic nanoparticles	685:707	We demonstrate here a universal strategy to encapsulate inorganic nanoparticles and to introduce different such inorganic nanoparticles into distinct polymer phases in Janus microparticles.
27031424	7	0	theme	55.0	1760:1763	arg1	%					1773:1773	55.0 and 48.3%	1760:1773	%	1773:1773	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	9	1	from	increase	2124:2131	arg1	expression					2189:2198	UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression	2136:2198	UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression	2136:2198	A longer term increase in UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression, probably mediated by a rise in MYBA1 was also observed.
27031424	3	2	from	content	553:559	arg1	berries					564:570	berries	564:570	berries	564:570	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	4	3	theme	genes	899:903	arg1	expression					881:890	the expression	877:890	the expression of key genes in the phenylpropanoid pathways	877:935	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	4	4	link	derived	751:757	arg1	PDO					777:779	PDO	777:779	PDO	777:779	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	4	4	link	derived	751:757	arg1	oligosaccharides					759:774	pectin derived oligosaccharides	744:774	pectin derived oligosaccharides (PDO)	744:780	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	6	5	theme	significant	1258:1268	arg1	rise					1270:1273	a significant rise	1256:1273	a significant rise in total anthocyanin content	1256:1302	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	10	6	located	observed	2313:2320	arg2	increase					2304:2311	the increase	2300:2311	the increase observed in MYB5A expression in PDO and Ethrel treatments	2300:2369	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	10	6	located	observed	2313:2320	arg1	expression					2331:2340	MYB5A expression	2325:2340	MYB5A expression in PDO and Ethrel treatments	2325:2369	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	11	7	theme	grape	2640:2644	arg1	anthocyanins					2652:2663	altering specific grape berry anthocyanins	2622:2663	altering specific grape berry anthocyanins	2622:2663	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	3	8	theme	positive	578:585	arg1	impact					587:592	a positive impact	576:592	a positive impact on the color	576:605	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	6	9	theme	acid	1491:1494	arg1	concentration					1496:1508	tartaric acid concentration	1482:1508	tartaric acid concentration	1482:1508	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	10	10	theme	flavonoid	2386:2394	arg1	3'-hydroxylase					2396:2409	flavonoid 3'-hydroxylase	2386:2409	flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT)	2386:2486	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	10	10	theme	flavonoid	2386:2394	arg1	F-3'-H					2412:2417	F-3'-H	2412:2417	F-3'-H	2412:2417	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	3	11	theme	total	608:612	arg1	concentration					623:635	total phenolic concentration	608:635	total phenolic concentration	608:635	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	10	12	theme	flavonoid	2421:2429	arg1	F-3'5'-H					2449:2456	F-3'5'-H	2449:2456	F-3'5'-H	2449:2456	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	10	12	theme	flavonoid	2421:2429	arg1	3'5'-hydroxylase					2431:2446	flavonoid 3'5'-hydroxylase	2421:2446	flavonoid 3'5'-hydroxylase (F-3'5'-H)	2421:2457	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	10	13	theme	profile	2514:2520	arg1	modifications					2522:2534	the profile modifications	2510:2534	the profile modifications described	2510:2544	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	6	14	theme	soluble	1466:1472	arg1	solids					1474:1479	soluble solids	1466:1479	soluble solids	1466:1479	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	11	15	theme	anthocyanins	2652:2663	arg1	means					2681:2685	a means	2679:2685	a means of positively influencing wine quality	2679:2724	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	11	15	theme	anthocyanins	2652:2663	arg1	application					2583:2593	application	2583:2593	application of PDO	2583:2600	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	11	15	theme	anthocyanins	2652:2663	arg1	means					2613:2617	a novel means	2605:2617	a novel means of altering specific grape berry anthocyanins	2605:2663	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	6	16	theme	e.Mv-3G/g	1393:1401	arg1	FW					1403:1404	1.02 mg e.Mv-3G/g FW	1385:1404	1.02 mg e.Mv-3G/g FW	1385:1404	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	7	17	from	increase	1624:1631	arg1	concentration					1649:1661	the relative concentration	1636:1661	the relative concentration of malvidin	1636:1673	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	11	18	theme	PDO	2598:2600	arg1	application					2583:2593	application	2583:2593	application of PDO	2583:2600	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	11	18	theme	PDO	2598:2600	arg1	means					2613:2617	a novel means	2605:2617	a novel means of altering specific grape berry anthocyanins	2605:2663	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	9	19	theme	term	2119:2122	arg1	increase					2124:2131	A longer term increase	2110:2131	A longer term increase in UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression, probably mediated by a rise in MYBA1	2110:2236	A longer term increase in UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression, probably mediated by a rise in MYBA1 was also observed.
27031424	6	20	theme	significant	1532:1542	arg1	alteration					1544:1553	a significant alteration	1530:1553	a significant alteration in the anthocyanin profile	1530:1580	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	4	21	theme	derived	751:757	arg1	PDO					777:779	PDO	777:779	PDO	777:779	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	4	21	theme	derived	751:757	arg1	oligosaccharides					759:774	pectin derived oligosaccharides	744:774	pectin derived oligosaccharides (PDO)	744:780	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	6	22	theme	anthocyanin	1284:1294	arg1	content					1296:1302	total anthocyanin content	1278:1302	total anthocyanin content	1278:1302	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	8	23	theme	MYB4A	2092:2096	arg1	expression					2098:2107	MYB4A expression	2092:2107	MYB4A expression	2092:2107	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	1	24	theme	secondary	159:167	arg1	Anthocyanins					142:153	Anthocyanins	142:153	Anthocyanins	142:153	Anthocyanins are secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway, with functions ranging from skin coloration to protection against pathogens or UV light.
27031424	1	24	theme	secondary	159:167	arg1	metabolites					169:179	secondary metabolites	159:179	secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway	159:244	Anthocyanins are secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway, with functions ranging from skin coloration to protection against pathogens or UV light.
27031424	9	25	theme	flavonoid	2148:2156	arg1	expression					2189:2198	UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression	2136:2198	UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression	2136:2198	A longer term increase in UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression, probably mediated by a rise in MYBA1 was also observed.
27031424	8	26	theme	total	1955:1959	arg1	content					1973:1979	total anthocyanin content	1955:1979	total anthocyanin content	1955:1979	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	6	27	theme	In	1517:1518	arg1	addition					1520:1527	pH. In addition	1513:1527	pH. In addition	1513:1527	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	4	28	dep	synthesis	789:797	arg1	the					785:787	the	785:787	the	785:787	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	4	29	theme	pre-veraison	716:727	arg1	application					729:739	the pre-veraison application	712:739	the pre-veraison application of pectin derived oligosaccharides (PDO)	712:780	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	8	30	theme	gene	1900:1903	arg1	analyses					1916:1923	gene expression analyses	1900:1923	gene expression analyses	1900:1923	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	1	31	theme	grape	196:200	arg1	skins					208:212	grape berry skins	196:212	grape berry skins	196:212	Anthocyanins are secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway, with functions ranging from skin coloration to protection against pathogens or UV light.
27031424	7	32	theme	malvidin	1666:1673	arg1	concentration					1649:1661	the relative concentration	1636:1661	the relative concentration of malvidin	1636:1673	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	8	33	from	increment	1942:1950	arg1	content					1973:1979	total anthocyanin content	1955:1979	total anthocyanin content	1955:1979	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	7	34	dep	PDO	1697:1699	arg1	treatments					1712:1721	treatments	1712:1721	treatments	1712:1721	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	8	35	theme	analyses	1916:1923	arg1	results					1889:1895	The results	1885:1895	The results of gene expression analyses	1885:1923	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	5	36	theme	gene	1108:1111	arg1	expression					1113:1122	gene expression	1108:1122	gene expression (by qRT-PCR)	1108:1135	To this end, pre-veraison Cabernet Sauvignon bunches were treated with PDO to subsequently determine total anthocyanin content, the anthocyanin profile (by HPLC-DAD) and gene expression (by qRT-PCR), using Ethrel and water treatments for comparison.
27031424	6	37	from	alteration	1544:1553	arg1	profile					1574:1580	the anthocyanin profile	1558:1580	the anthocyanin profile	1558:1580	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	2	38	theme	viticultural	436:447	arg1	practices					449:457	viticultural practices	436:457	viticultural practices	436:457	Accumulation of these compounds is highly variable depending on genetics, environmental factors and viticultural practices.
27031424	0	39	theme	gene	125:128	arg1	expression					130:139	gene expression	125:139	gene expression	125:139	Exogenous application of pectin-derived oligosaccharides to grape berries modifies anthocyanin accumulation, composition and gene expression.
27031424	3	40	from	price	658:662	arg1	color					601:605	the color	597:605	the color	597:605	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	3	41	theme	biological	474:483	arg1	functions					485:493	their biological functions	468:493	their biological functions	468:493	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	0	42	theme	Exogenous	0:8	arg1	application					10:20	Exogenous application	0:20	Exogenous application of pectin-derived oligosaccharides to grape berries	0:72	Exogenous application of pectin-derived oligosaccharides to grape berries modifies anthocyanin accumulation, composition and gene expression.
27031424	6	43	theme	anthocyanin	1562:1572	arg1	profile					1574:1580	the anthocyanin profile	1558:1580	the anthocyanin profile	1558:1580	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	5	44	theme	pre-veraison	951:962	arg1	bunches					983:989	pre-veraison Cabernet Sauvignon bunches	951:989	pre-veraison Cabernet Sauvignon bunches	951:989	To this end, pre-veraison Cabernet Sauvignon bunches were treated with PDO to subsequently determine total anthocyanin content, the anthocyanin profile (by HPLC-DAD) and gene expression (by qRT-PCR), using Ethrel and water treatments for comparison.
27031424	8	45	theme	term	2003:2006	arg1	increase					2008:2015	a short term increase	1995:2015	a short term increase	1995:2015	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	11	46	theme	agrochemicals	2750:2762	arg1	addition					2738:2745	the addition	2734:2745	the addition of agrochemicals	2734:2762	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	3	47	from	concentration	623:635	arg1	color					601:605	the color	597:605	the color	597:605	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	6	48	theme	1.02 mg	1385:1391	arg1	FW					1403:1404	1.02 mg e.Mv-3G/g FW	1385:1404	1.02 mg e.Mv-3G/g FW	1385:1404	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	1	49	theme	UV	326:327	arg1	light					329:333	UV light	326:333	UV light	326:333	Anthocyanins are secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway, with functions ranging from skin coloration to protection against pathogens or UV light.
27031424	6	50	dep	1.32	1370:1373	arg1	1.48					1376:1379	1.48	1376:1379	1.48	1376:1379	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	6	50	dep	1.32	1370:1373	arg1	FW					1403:1404	1.02 mg e.Mv-3G/g FW	1385:1404	1.02 mg e.Mv-3G/g FW	1385:1404	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	5	51	theme	Sauvignon	973:981	arg1	bunches					983:989	pre-veraison Cabernet Sauvignon bunches	951:989	pre-veraison Cabernet Sauvignon bunches	951:989	To this end, pre-veraison Cabernet Sauvignon bunches were treated with PDO to subsequently determine total anthocyanin content, the anthocyanin profile (by HPLC-DAD) and gene expression (by qRT-PCR), using Ethrel and water treatments for comparison.
27031424	0	52	theme	oligosaccharides	40:55	arg1	application					10:20	Exogenous application	0:20	Exogenous application of pectin-derived oligosaccharides to grape berries	0:72	Exogenous application of pectin-derived oligosaccharides to grape berries modifies anthocyanin accumulation, composition and gene expression.
27031424	5	53	theme	anthocyanin	1045:1055	arg1	content					1057:1063	total anthocyanin content	1039:1063	total anthocyanin content	1039:1063	To this end, pre-veraison Cabernet Sauvignon bunches were treated with PDO to subsequently determine total anthocyanin content, the anthocyanin profile (by HPLC-DAD) and gene expression (by qRT-PCR), using Ethrel and water treatments for comparison.
27031424	8	54	from	increase	2008:2015	arg1	expression					2054:2063	phenylalanine ammonia-lyase (PAL) expression	2020:2063	phenylalanine ammonia-lyase (PAL) expression	2020:2063	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	8	55	theme	ammonia-lyase	2034:2046	arg1	expression					2054:2063	phenylalanine ammonia-lyase (PAL) expression	2020:2063	phenylalanine ammonia-lyase (PAL) expression	2020:2063	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	3	56	theme	wine	667:670	arg1	impact					587:592	a positive impact	576:592	a positive impact on the color	576:605	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	3	56	theme	wine	667:670	arg1	concentration					623:635	total phenolic concentration	608:635	total phenolic concentration	608:635	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	3	56	theme	wine	667:670	arg1	price					658:662	the price	654:662	the price of wine	654:670	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	3	57	theme	high	536:539	arg1	content					553:559	a high anthocyanin content	534:559	a high anthocyanin content in berries	534:570	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	0	58	theme	grape	60:64	arg1	berries					66:72	grape berries	60:72	grape berries	60:72	Exogenous application of pectin-derived oligosaccharides to grape berries modifies anthocyanin accumulation, composition and gene expression.
27031424	5	59	theme	water	1155:1159	arg1	treatments					1161:1170	water treatments	1155:1170	water treatments for comparison	1155:1185	To this end, pre-veraison Cabernet Sauvignon bunches were treated with PDO to subsequently determine total anthocyanin content, the anthocyanin profile (by HPLC-DAD) and gene expression (by qRT-PCR), using Ethrel and water treatments for comparison.
27031424	7	60	theme	significant	1798:1808	arg1	rise					1810:1813	a significant rise	1796:1813	a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins	1796:1882	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	10	61	dep	3'-hydroxylase	2396:2409	arg1	F-3'5'-H					2449:2456	F-3'5'-H	2449:2456	F-3'5'-H	2449:2456	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	10	61	dep	3'-hydroxylase	2396:2409	arg1	O-methyltransferase					2462:2480	O-methyltransferase	2462:2480	O-methyltransferase (OMT)	2462:2486	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	10	61	dep	3'-hydroxylase	2396:2409	arg1	3'5'-hydroxylase					2431:2446	flavonoid 3'5'-hydroxylase	2421:2446	flavonoid 3'5'-hydroxylase (F-3'5'-H)	2421:2457	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	10	61	dep	3'-hydroxylase	2396:2409	arg1	OMT					2483:2485	OMT	2483:2485	OMT	2483:2485	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	11	62	theme	wine	2713:2716	arg1	quality					2718:2724	positively influencing wine quality	2690:2724	positively influencing wine quality	2690:2724	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	6	63	from	effect	1444:1449	arg1	solids					1474:1479	soluble solids	1466:1479	soluble solids	1466:1479	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	6	63	from	effect	1444:1449	arg1	addition					1520:1527	pH. In addition	1513:1527	pH. In addition	1513:1527	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	6	63	from	effect	1444:1449	arg1	concentration					1496:1508	tartaric acid concentration	1482:1508	tartaric acid concentration	1482:1508	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	6	63	from	effect	1444:1449	arg1	size					1460:1463	berry size	1454:1463	berry size	1454:1463	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	8	64	theme	anthocyanin	1961:1971	arg1	content					1973:1979	total anthocyanin content	1955:1979	total anthocyanin content	1955:1979	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	6	65	theme	berry	1454:1458	arg1	size					1460:1463	berry size	1454:1463	berry size	1454:1463	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	6	66	from	rise	1270:1273	arg1	content					1296:1302	total anthocyanin content	1278:1302	total anthocyanin content	1278:1302	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	4	67	theme	compounds	825:833	arg1	accumulation					803:814	accumulation	803:814	accumulation	803:814	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	4	67	theme	compounds	825:833	arg1	synthesis					789:797	synthesis	789:797	synthesis	789:797	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	11	68	theme	berry	2646:2650	arg1	anthocyanins					2652:2663	altering specific grape berry anthocyanins	2622:2663	altering specific grape berry anthocyanins	2622:2663	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	10	69	with	related	2497:2503	arg1	modifications					2522:2534	the profile modifications	2510:2534	the profile modifications described	2510:2544	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	8	70	theme	expression	1905:1914	arg1	analyses					1916:1923	gene expression analyses	1900:1923	gene expression analyses	1900:1923	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	6	71	theme	total	1278:1282	arg1	content					1296:1302	total anthocyanin content	1278:1302	total anthocyanin content	1278:1302	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	0	72	link	pectin-derived	25:38	arg1	oligosaccharides					40:55	pectin-derived oligosaccharides	25:55	pectin-derived oligosaccharides	25:55	Exogenous application of pectin-derived oligosaccharides to grape berries modifies anthocyanin accumulation, composition and gene expression.
27031424	4	73	from	effect	702:707	arg1	accumulation					803:814	accumulation	803:814	accumulation	803:814	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	4	73	from	effect	702:707	arg1	synthesis					789:797	synthesis	789:797	synthesis	789:797	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	4	74	theme	key	895:897	arg1	genes					899:903	key genes	895:903	key genes	895:903	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	7	75	theme	water	1738:1742	arg1	controls					1744:1751	water controls	1738:1751	water controls (52.8; 55.0 and 48.3%, respectively)	1738:1788	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	11	76	theme	altering	2622:2629	arg1	anthocyanins					2652:2663	altering specific grape berry anthocyanins	2622:2663	altering specific grape berry anthocyanins	2622:2663	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	1	77	theme	berry	202:206	arg1	skins					208:212	grape berry skins	196:212	grape berry skins	196:212	Anthocyanins are secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway, with functions ranging from skin coloration to protection against pathogens or UV light.
27031424	8	78	from	decrease	2080:2087	arg1	expression					2098:2107	MYB4A expression	2092:2107	MYB4A expression	2092:2107	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	4	79	theme	present	677:683	arg1	work					685:688	The present work	673:688	The present work	673:688	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	6	80	theme	tartaric	1482:1489	arg1	concentration					1496:1508	tartaric acid concentration	1482:1508	tartaric acid concentration	1482:1508	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	11	81	theme	specific	2631:2638	arg1	anthocyanins					2652:2663	altering specific grape berry anthocyanins	2622:2663	altering specific grape berry anthocyanins	2622:2663	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	1	82	theme	phenylpropanoid	222:236	arg1	pathway					238:244	the phenylpropanoid pathway	218:244	the phenylpropanoid pathway	218:244	Anthocyanins are secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway, with functions ranging from skin coloration to protection against pathogens or UV light.
27031424	11	83	theme	novel	2607:2611	arg1	application					2583:2593	application	2583:2593	application of PDO	2583:2600	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	11	83	theme	novel	2607:2611	arg1	means					2613:2617	a novel means	2605:2617	a novel means of altering specific grape berry anthocyanins	2605:2663	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	3	84	theme	phenolic	614:621	arg1	concentration					623:635	total phenolic concentration	608:635	total phenolic concentration	608:635	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	10	85	theme	anthocyanin	2271:2281	arg1	profile					2283:2289	the anthocyanin profile	2267:2289	the anthocyanin profile	2267:2289	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	4	86	theme	application	729:739	arg1	effect					702:707	the effect	698:707	the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds	698:833	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	5	87	theme	anthocyanin	1070:1080	arg1	profile					1082:1088	the anthocyanin profile	1066:1088	the anthocyanin profile (by HPLC-DAD)	1066:1102	To this end, pre-veraison Cabernet Sauvignon bunches were treated with PDO to subsequently determine total anthocyanin content, the anthocyanin profile (by HPLC-DAD) and gene expression (by qRT-PCR), using Ethrel and water treatments for comparison.
27031424	9	88	from	rise	2224:2227	arg1	MYBA1					2232:2236	MYBA1	2232:2236	MYBA1	2232:2236	A longer term increase in UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression, probably mediated by a rise in MYBA1 was also observed.
27031424	4	89	theme	phenylpropanoid	912:926	arg1	pathways					928:935	the phenylpropanoid pathways	908:935	the phenylpropanoid pathways	908:935	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	10	90	from	expression	2331:2340	arg1	Ethrel					2353:2358	Ethrel	2353:2358	Ethrel	2353:2358	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	10	90	from	expression	2331:2340	arg1	PDO					2345:2347	PDO	2345:2347	PDO	2345:2347	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	9	91	theme	UDP-glucose	2136:2146	arg1	expression					2189:2198	UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression	2136:2198	UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression	2136:2198	A longer term increase in UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression, probably mediated by a rise in MYBA1 was also observed.
27031424	0	92	theme	anthocyanin	83:93	arg1	accumulation					95:106	anthocyanin accumulation	83:106	anthocyanin accumulation	83:106	Exogenous application of pectin-derived oligosaccharides to grape berries modifies anthocyanin accumulation, composition and gene expression.
27031424	9	93	theme	3-O-glucosyltransferase	2158:2180	arg1	expression					2189:2198	UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression	2136:2198	UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression	2136:2198	A longer term increase in UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression, probably mediated by a rise in MYBA1 was also observed.
27031424	6	94	theme	water	1352:1356	arg1	treatments					1358:1367	water treatments	1352:1367	water treatments	1352:1367	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	6	95	theme	pH.	1513:1515	arg1	addition					1520:1527	pH. In addition	1513:1527	pH. In addition	1513:1527	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	7	96	from	rise	1810:1813	arg1	a					1845:1845	a	1845:1845	a	1845:1845	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	7	96	from	rise	1810:1813	arg1	forms					1835:1839	tri-hydroxylated forms	1818:1839	tri-hydroxylated forms	1818:1839	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	3	97	contain	has	572:574	arg2	price					658:662	the price	654:662	the price of wine	654:670	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	3	97	contain	has	572:574	arg2	concentration					623:635	total phenolic concentration	608:635	total phenolic concentration	608:635	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	3	97	contain	has	572:574	arg1	content					553:559	a high anthocyanin content	534:559	a high anthocyanin content in berries	534:570	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	3	97	contain	has	572:574	arg2	impact					587:592	a positive impact	576:592	a positive impact on the color	576:605	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	6	98	dep	efficient	1222:1230	arg1	1.32					1370:1373	1.32	1370:1373	1.32	1370:1373	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	6	98	dep	efficient	1222:1230	arg1	compared					1338:1345	compared	1338:1345	compared with water treatments	1338:1367	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	3	99	from	impact	587:592	arg1	color					601:605	the color	597:605	the color	597:605	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	9	100	theme	UFGT	2183:2186	arg1	expression					2189:2198	UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression	2136:2198	UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression	2136:2198	A longer term increase in UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) expression, probably mediated by a rise in MYBA1 was also observed.
27031424	4	101	from	expression	881:890	arg1	pathways					928:935	the phenylpropanoid pathways	908:935	the phenylpropanoid pathways	908:935	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	1	102	with	metabolites	169:179	arg1	functions					252:260	functions	252:260	functions ranging from skin coloration to protection against pathogens or UV light	252:333	Anthocyanins are secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway, with functions ranging from skin coloration to protection against pathogens or UV light.
27031424	10	103	theme	MYB5A	2325:2329	arg1	expression					2331:2340	MYB5A expression	2325:2340	MYB5A expression in PDO and Ethrel treatments	2325:2369	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	10	104	dep	PDO	2345:2347	arg1	treatments					2360:2369	treatments	2360:2369	treatments	2360:2369	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	8	105	theme	short	1997:2001	arg1	increase					2008:2015	a short term increase	1995:2015	a short term increase	1995:2015	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	10	106	from	changes	2375:2381	arg1	3'-hydroxylase					2396:2409	flavonoid 3'-hydroxylase	2386:2409	flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT)	2386:2486	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	10	106	from	changes	2375:2381	arg1	F-3'-H					2412:2417	F-3'-H	2412:2417	F-3'-H	2412:2417	Regarding the anthocyanin profile, despite the increase observed in MYB5A expression in PDO and Ethrel treatments, no changes in flavonoid 3'-hydroxylase (F-3'-H); flavonoid 3'5'-hydroxylase (F-3'5'-H) or O-methyltransferase (OMT) could be related with the profile modifications described.
27031424	4	107	dep	derived	751:757	arg1	pectin					744:749	pectin	744:749	pectin	744:749	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	7	108	theme	relative	1640:1647	arg1	concentration					1649:1661	the relative concentration	1636:1661	the relative concentration of malvidin	1636:1673	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	4	109	theme	oligosaccharides	759:774	arg1	application					729:739	the pre-veraison application	712:739	the pre-veraison application of pectin derived oligosaccharides (PDO)	712:780	The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways.
27031424	2	110	theme	environmental	410:422	arg1	factors					424:430	environmental factors	410:430	environmental factors	410:430	Accumulation of these compounds is highly variable depending on genetics, environmental factors and viticultural practices.
27031424	0	111	theme	pectin-derived	25:38	arg1	oligosaccharides					40:55	pectin-derived oligosaccharides	25:55	pectin-derived oligosaccharides	25:55	Exogenous application of pectin-derived oligosaccharides to grape berries modifies anthocyanin accumulation, composition and gene expression.
27031424	7	112	theme	tri-hydroxylated	1818:1833	arg1	forms					1835:1839	tri-hydroxylated forms	1818:1839	tri-hydroxylated forms	1818:1839	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	5	113	theme	Cabernet	964:971	arg1	bunches					983:989	pre-veraison Cabernet Sauvignon bunches	951:989	pre-veraison Cabernet Sauvignon bunches	951:989	To this end, pre-veraison Cabernet Sauvignon bunches were treated with PDO to subsequently determine total anthocyanin content, the anthocyanin profile (by HPLC-DAD) and gene expression (by qRT-PCR), using Ethrel and water treatments for comparison.
27031424	8	114	theme	phenylalanine	2020:2032	arg1	PAL					2049:2051	PAL	2049:2051	PAL	2049:2051	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	8	114	theme	phenylalanine	2020:2032	arg1	ammonia-lyase					2034:2046	phenylalanine ammonia-lyase	2020:2046	phenylalanine ammonia-lyase (PAL) expression	2020:2063	The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression.
27031424	5	115	theme	total	1039:1043	arg1	content					1057:1063	total anthocyanin content	1039:1063	total anthocyanin content	1039:1063	To this end, pre-veraison Cabernet Sauvignon bunches were treated with PDO to subsequently determine total anthocyanin content, the anthocyanin profile (by HPLC-DAD) and gene expression (by qRT-PCR), using Ethrel and water treatments for comparison.
27031424	7	116	theme	significant	1612:1622	arg1	increase					1624:1631	a significant increase	1610:1631	a significant increase in the relative concentration of malvidin	1610:1673	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	7	117	dep	a	1845:1845	arg1	fall					1847:1850	fall	1847:1850	fall in di-hydroxylated anthocyanins	1847:1882	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	7	118	theme	di-hydroxylated	1855:1869	arg1	anthocyanins					1871:1882	di-hydroxylated anthocyanins	1855:1882	di-hydroxylated anthocyanins	1855:1882	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	3	119	theme	wine	517:520	arg1	quality					522:528	wine quality	517:528	wine quality	517:528	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	3	120	theme	anthocyanin	541:551	arg1	content					553:559	a high anthocyanin content	534:559	a high anthocyanin content in berries	534:570	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	3	121	dep	improve	509:515	arg1	as					531:532	as	531:532	as	531:532	Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine.
27031424	7	122	dep	controls	1744:1751	arg1	52.8					1754:1757	52.8	1754:1757	52.8	1754:1757	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	11	123	theme	quality	2718:2724	arg1	means					2681:2685	a means	2679:2685	a means of positively influencing wine quality	2679:2724	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	11	123	theme	quality	2718:2724	arg1	application					2583:2593	application	2583:2593	application of PDO	2583:2600	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	11	123	theme	quality	2718:2724	arg1	means					2613:2617	a novel means	2605:2617	a novel means of altering specific grape berry anthocyanins	2605:2663	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	2	124	theme	compounds	358:366	arg1	Accumulation					336:347	Accumulation	336:347	Accumulation of these compounds	336:366	Accumulation of these compounds is highly variable depending on genetics, environmental factors and viticultural practices.
27031424	7	125	theme	48.3	1769:1772	arg1	%					1773:1773	55.0 and 48.3%	1760:1773	%	1773:1773	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	7	126	dep	52.8	1754:1757	arg1	%					1773:1773	55.0 and 48.3%	1760:1773	%	1773:1773	Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins.
27031424	11	127	theme	influencing	2701:2711	arg1	quality					2718:2724	positively influencing wine quality	2690:2724	positively influencing wine quality	2690:2724	Overall, this study highlights that application of PDO is a novel means of altering specific grape berry anthocyanins, and could be a means of positively influencing wine quality without the addition of agrochemicals.
27031424	6	128	theme	undesirable	1432:1442	arg1	effect					1444:1449	any undesirable effect	1428:1449	any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition	1428:1527	The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed.
27031424	1	129	theme	skin	275:278	arg1	coloration					280:289	skin coloration	275:289	skin coloration	275:289	Anthocyanins are secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway, with functions ranging from skin coloration to protection against pathogens or UV light.
28084000	5	0	theme	polymer	1124:1130	arg1	formulations					1132:1143	Freeze-dried polymer formulations	1111:1143	Freeze-dried polymer formulations containing low and medium chitosan Mn and concentrations	1111:1200	Freeze-dried polymer formulations containing low and medium chitosan Mn and concentrations were rapidly and completely solubilized in PRP.
28084000	6	1	theme	solid	1314:1318	arg1	hybrids					1333:1339	solid chitosan-PRP hybrids	1314:1339	solid chitosan-PRP hybrids	1314:1339	The paste-like chitosan-PRP mixtures coagulated quickly to form solid chitosan-PRP hybrids, which retracted much less than PRP-only controls.
28084000	10	2	dep	&	1903:1903	arg1	Sons					1905:1908	Sons	1905:1908	Sons	1905:1908	Copyright © 2017 John Wiley & Sons, Ltd.
28084000	6	3	dep	mixtures	1278:1285	arg1	coagulated					1287:1296	coagulated	1287:1296	mixtures coagulated quickly to form solid chitosan-PRP hybrids, which retracted much less than PRP-only controls	1278:1389	The paste-like chitosan-PRP mixtures coagulated quickly to form solid chitosan-PRP hybrids, which retracted much less than PRP-only controls.
28084000	1	4	theme	tissue	335:340	arg1	repair					342:347	tissue repair	335:347	tissue repair	335:347	The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair.
28084000	4	5	theme	dorsal	1048:1053	arg1	injections					1068:1077	dorsal subcutaneous injections	1048:1077	dorsal subcutaneous injections into New Zealand White rabbits	1048:1108	PRP was used to solubilize the freeze-dried cakes and assess in vitro and in vivo performance, the latter as dorsal subcutaneous injections into New Zealand White rabbits.
28084000	0	6	theme	in	103:104	arg1	properties					91:100	in vitro properties	82:100	in vitro properties	82:100	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	0	6	theme	in	103:104	arg1	residence					111:119	residence	111:119	residence	111:119	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	8	7	theme	Chitosan-PRP	1534:1545	arg1	clots					1554:1558	Chitosan-PRP hybrid clots	1534:1558	Chitosan-PRP hybrid clots	1534:1558	Chitosan-PRP hybrid clots were resident subcutaneously in vivo until at least 2 weeks while PRP controls were quickly degraded in one day.
28084000	4	8	dep	in	1000:1001	arg1	vitro					1003:1007	vitro	1003:1007	vitro	1003:1007	PRP was used to solubilize the freeze-dried cakes and assess in vitro and in vivo performance, the latter as dorsal subcutaneous injections into New Zealand White rabbits.
28084000	2	9	theme	handling	639:646	arg1	properties					648:657	paste-like handling properties	628:657	paste-like handling properties	628:657	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	3	10	theme	calcium	816:822	arg1	chloride					824:831	calcium chloride	816:831	calcium chloride	816:831	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	9	11	from	use	1845:1847	arg1	medicine					1865:1872	regenerative medicine	1852:1872	regenerative medicine	1852:1872	Compared to PRP alone, chitosan-PRP hybrids had much greater capacity to induce local cell recruitment accompanied by angiogenesis, suggesting a strong potential for their use in regenerative medicine.
28084000	7	12	theme	chitosan	1477:1484	arg1	Mn					1486:1487	chitosan Mn	1477:1487	chitosan Mn	1477:1487	Homogeneous dispersion of chitosan within the hybrid clots was strongly dependent on chitosan Mn , and occurred only with medium Mn chitosan.
28084000	2	13	theme	paste-like	628:637	arg1	properties					648:657	paste-like handling properties	628:657	paste-like handling properties	628:657	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	7	14	theme	medium	1514:1519	arg1	chitosan					1524:1531	medium Mn chitosan	1514:1531	medium Mn chitosan	1514:1531	Homogeneous dispersion of chitosan within the hybrid clots was strongly dependent on chitosan Mn , and occurred only with medium Mn chitosan.
28084000	3	15	theme	lyoprotectant	895:907	arg1	concentration					909:921	lyoprotectant concentration	895:921	lyoprotectant concentration	895:921	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	3	15	theme	lyoprotectant	895:907	arg1	Mn					863:864	distinct chitosan Mn	845:864	distinct chitosan Mn	845:864	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	9	16	theme	strong	1818:1823	arg1	potential					1825:1833	a strong potential	1816:1833	a strong potential for their use in regenerative medicine	1816:1872	Compared to PRP alone, chitosan-PRP hybrids had much greater capacity to induce local cell recruitment accompanied by angiogenesis, suggesting a strong potential for their use in regenerative medicine.
28084000	0	17	dep	in	103:104	arg1	vivo					106:109	vivo	106:109	vivo	106:109	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	4	18	theme	New	1084:1086	arg1	rabbits					1102:1108	New Zealand White rabbits	1084:1108	New Zealand White rabbits	1084:1108	PRP was used to solubilize the freeze-dried cakes and assess in vitro and in vivo performance, the latter as dorsal subcutaneous injections into New Zealand White rabbits.
28084000	6	19	theme	paste-like	1254:1263	arg1	chitosan-PRP					1265:1276	The paste-like chitosan-PRP	1250:1276	The paste-like chitosan-PRP	1250:1276	The paste-like chitosan-PRP mixtures coagulated quickly to form solid chitosan-PRP hybrids, which retracted much less than PRP-only controls.
28084000	6	20	theme	PRP-only	1373:1380	arg1	controls					1382:1389	PRP-only controls	1373:1389	PRP-only controls	1373:1389	The paste-like chitosan-PRP mixtures coagulated quickly to form solid chitosan-PRP hybrids, which retracted much less than PRP-only controls.
28084000	5	21	dep	low	1156:1158	arg1	concentrations					1187:1200	concentrations	1187:1200	concentrations	1187:1200	Freeze-dried polymer formulations containing low and medium chitosan Mn and concentrations were rapidly and completely solubilized in PRP.
28084000	5	21	dep	low	1156:1158	arg1	Mn					1180:1181	chitosan Mn	1171:1181	chitosan Mn	1171:1181	Freeze-dried polymer formulations containing low and medium chitosan Mn and concentrations were rapidly and completely solubilized in PRP.
28084000	4	22	theme	in	1000:1001	arg1	performance					1021:1031	in vitro and in vivo performance	1000:1031	in vitro and in vivo performance	1000:1031	PRP was used to solubilize the freeze-dried cakes and assess in vitro and in vivo performance, the latter as dorsal subcutaneous injections into New Zealand White rabbits.
28084000	4	23	theme	in	1013:1014	arg1	performance					1021:1031	in vitro and in vivo performance	1000:1031	in vitro and in vivo performance	1000:1031	PRP was used to solubilize the freeze-dried cakes and assess in vitro and in vivo performance, the latter as dorsal subcutaneous injections into New Zealand White rabbits.
28084000	2	24	theme	hybrid	738:743	arg1	implants					745:752	solid chitosan-PRP hybrid implants	719:752	solid chitosan-PRP hybrid implants that are stable and homogenous	719:783	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	25	theme	formulation	382:392	arg1	concentration					467:479	chitosan concentration	458:479	chitosan concentration	458:479	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	25	theme	formulation	382:392	arg1	parameters					394:403	formulation parameters	382:403	formulation parameters	382:403	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	25	theme	formulation	382:392	arg1	number					425:430	chitosan number average molar mass (Mn )	416:455	chitosan number average molar mass (Mn )	416:455	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	25	theme	formulation	382:392	arg1	concentration					499:511	lyoprotectant concentration	485:511	lyoprotectant concentration	485:511	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	26	contain	have	623:626	arg2	properties					648:657	paste-like handling properties	628:657	paste-like handling properties	628:657	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	26	contain	have	623:626	arg1	compositions					541:552	compositions	541:552	compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous	541:783	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	9	27	theme	regenerative	1852:1863	arg1	medicine					1865:1872	regenerative medicine	1852:1872	regenerative medicine	1852:1872	Compared to PRP alone, chitosan-PRP hybrids had much greater capacity to induce local cell recruitment accompanied by angiogenesis, suggesting a strong potential for their use in regenerative medicine.
28084000	4	28	theme	freeze-dried	970:981	arg1	cakes					983:987	the freeze-dried cakes	966:987	the freeze-dried cakes	966:987	PRP was used to solubilize the freeze-dried cakes and assess in vitro and in vivo performance, the latter as dorsal subcutaneous injections into New Zealand White rabbits.
28084000	2	29	theme	chitosan-PRP	725:736	arg1	implants					745:752	solid chitosan-PRP hybrid implants	719:752	solid chitosan-PRP hybrid implants that are stable and homogenous	719:783	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	0	30	theme	Injectable	0:9	arg1	implants					41:48	Injectable chitosan-platelet-rich plasma implants	0:48	Injectable chitosan-platelet-rich plasma implants	0:48	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	1	31	theme	platelet-rich	276:288	arg1	PRP					298:300	PRP	298:300	PRP	298:300	The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair.
28084000	1	31	theme	platelet-rich	276:288	arg1	plasma					290:295	platelet-rich plasma	276:295	platelet-rich plasma (PRP)	276:301	The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair.
28084000	4	32	dep	in	1013:1014	arg1	vivo					1016:1019	vivo	1016:1019	vivo	1016:1019	PRP was used to solubilize the freeze-dried cakes and assess in vitro and in vivo performance, the latter as dorsal subcutaneous injections into New Zealand White rabbits.
28084000	7	33	theme	Mn	1521:1522	arg1	chitosan					1524:1531	medium Mn chitosan	1514:1531	medium Mn chitosan	1514:1531	Homogeneous dispersion of chitosan within the hybrid clots was strongly dependent on chitosan Mn , and occurred only with medium Mn chitosan.
28084000	4	34	theme	White	1096:1100	arg1	rabbits					1102:1108	New Zealand White rabbits	1084:1108	New Zealand White rabbits	1084:1108	PRP was used to solubilize the freeze-dried cakes and assess in vitro and in vivo performance, the latter as dorsal subcutaneous injections into New Zealand White rabbits.
28084000	2	35	theme	molar	440:444	arg1	mass					446:449	average molar mass	432:449	chitosan number average molar mass (Mn )	416:455	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	35	theme	molar	440:444	arg1	Mn					452:453	Mn	452:453	Mn	452:453	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	0	36	dep	in	82:83	arg1	vitro					85:89	vitro	85:89	vitro	85:89	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	4	37	theme	Zealand	1088:1094	arg1	rabbits					1102:1108	New Zealand White rabbits	1084:1108	New Zealand White rabbits	1084:1108	PRP was used to solubilize the freeze-dried cakes and assess in vitro and in vivo performance, the latter as dorsal subcutaneous injections into New Zealand White rabbits.
28084000	1	38	theme	study	193:197	arg1	purpose					177:183	The purpose	173:183	The purpose of this study	173:197	The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair.
28084000	0	39	theme	plasma	34:39	arg1	implants					41:48	Injectable chitosan-platelet-rich plasma implants	0:48	Injectable chitosan-platelet-rich plasma implants	0:48	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	2	40	dep	approach	363:370	arg1	adjust					375:380	adjust	375:380	to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration,	372:512	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	7	41	theme	hybrid	1438:1443	arg1	clots					1445:1449	the hybrid clots	1434:1449	the hybrid clots	1434:1449	Homogeneous dispersion of chitosan within the hybrid clots was strongly dependent on chitosan Mn , and occurred only with medium Mn chitosan.
28084000	2	42	theme	chitosan	416:423	arg1	number					425:430	chitosan number average molar mass (Mn )	416:455	chitosan number average molar mass (Mn )	416:455	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	43	theme	chitosan	458:465	arg1	concentration					467:479	chitosan concentration	458:479	chitosan concentration	458:479	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	0	44	theme	cell	135:138	arg1	properties					91:100	in vitro properties	82:100	in vitro properties	82:100	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	0	44	theme	cell	135:138	arg1	recruitment					140:150	cell recruitment	135:150	cell recruitment	135:150	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	7	45	theme	chitosan	1418:1425	arg1	dispersion					1404:1413	Homogeneous dispersion	1392:1413	Homogeneous dispersion of chitosan within the hybrid clots	1392:1449	Homogeneous dispersion of chitosan within the hybrid clots was strongly dependent on chitosan Mn , and occurred only with medium Mn chitosan.
28084000	2	46	dep	number	425:430	arg1	mass					446:449	average molar mass	432:449	chitosan number average molar mass (Mn )	416:455	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	46	dep	number	425:430	arg1	Mn					452:453	Mn	452:453	Mn	452:453	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	9	47	theme	greater	1726:1732	arg1	capacity					1734:1741	much greater capacity	1721:1741	much greater capacity to induce local cell recruitment accompanied by angiogenesis	1721:1802	Compared to PRP alone, chitosan-PRP hybrids had much greater capacity to induce local cell recruitment accompanied by angiogenesis, suggesting a strong potential for their use in regenerative medicine.
28084000	5	48	theme	chitosan	1171:1178	arg1	Mn					1180:1181	chitosan Mn	1171:1181	chitosan Mn	1171:1181	Freeze-dried polymer formulations containing low and medium chitosan Mn and concentrations were rapidly and completely solubilized in PRP.
28084000	5	49	contain	containing	1145:1154	arg2	low					1156:1158	low	1156:1158	low	1156:1158	Freeze-dried polymer formulations containing low and medium chitosan Mn and concentrations were rapidly and completely solubilized in PRP.
28084000	5	49	contain	containing	1145:1154	arg1	formulations					1132:1143	Freeze-dried polymer formulations	1111:1143	Freeze-dried polymer formulations containing low and medium chitosan Mn and concentrations	1111:1200	Freeze-dried polymer formulations containing low and medium chitosan Mn and concentrations were rapidly and completely solubilized in PRP.
28084000	5	49	contain	containing	1145:1154	arg2	medium					1164:1169	medium	1164:1169	medium	1164:1169	Freeze-dried polymer formulations containing low and medium chitosan Mn and concentrations were rapidly and completely solubilized in PRP.
28084000	2	50	dep	rapidly	565:571	arg1	1 min					576:580	< 1 min	574:580	< 1 min	574:580	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	0	51	dep	promote	53:59	arg1	properties					91:100	in vitro properties	82:100	in vitro properties	82:100	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	0	51	dep	promote	53:59	arg1	vascularization					156:170	vascularization	156:170	vascularization	156:170	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	0	51	dep	promote	53:59	arg1	degradation					122:132	degradation	122:132	degradation	122:132	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	0	51	dep	promote	53:59	arg1	recruitment					140:150	cell recruitment	135:150	cell recruitment	135:150	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	0	51	dep	promote	53:59	arg1	residence					111:119	residence	111:119	residence	111:119	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	2	52	theme	<	702:702	arg1	5 min					704:708	< 5 min	702:708	< 5 min	702:708	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	0	53	theme	tissue	61:66	arg1	regeneration					68:79	tissue regeneration	61:79	tissue regeneration	61:79	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	8	54	theme	PRP	1626:1628	arg1	controls					1630:1637	PRP controls	1626:1637	PRP controls	1626:1637	Chitosan-PRP hybrid clots were resident subcutaneously in vivo until at least 2 weeks while PRP controls were quickly degraded in one day.
28084000	2	55	theme	<	574:574	arg1	1 min					576:580	< 1 min	574:580	< 1 min	574:580	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	9	56	theme	chitosan-PRP	1696:1707	arg1	hybrids					1709:1715	chitosan-PRP hybrids	1696:1715	chitosan-PRP hybrids	1696:1715	Compared to PRP alone, chitosan-PRP hybrids had much greater capacity to induce local cell recruitment accompanied by angiogenesis, suggesting a strong potential for their use in regenerative medicine.
28084000	8	57	theme	hybrid	1547:1552	arg1	clots					1554:1558	Chitosan-PRP hybrid clots	1534:1558	Chitosan-PRP hybrid clots	1534:1558	Chitosan-PRP hybrid clots were resident subcutaneously in vivo until at least 2 weeks while PRP controls were quickly degraded in one day.
28084000	3	58	theme	distinct	845:852	arg1	concentration					909:921	lyoprotectant concentration	895:921	lyoprotectant concentration	895:921	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	3	58	theme	distinct	845:852	arg1	Mn					863:864	distinct chitosan Mn	845:864	distinct chitosan Mn	845:864	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	3	58	theme	distinct	845:852	arg1	concentration					877:889	chitosan concentration	868:889	chitosan concentration	868:889	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	1	59	theme	injectable	311:320	arg1	implants					322:329	injectable implants	311:329	injectable implants for tissue repair	311:347	The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair.
28084000	9	60	theme	cell	1759:1762	arg1	recruitment					1764:1774	local cell recruitment	1753:1774	local cell recruitment accompanied by angiogenesis	1753:1802	Compared to PRP alone, chitosan-PRP hybrids had much greater capacity to induce local cell recruitment accompanied by angiogenesis, suggesting a strong potential for their use in regenerative medicine.
28084000	9	61	theme	local	1753:1757	arg1	recruitment					1764:1774	local cell recruitment	1753:1774	local cell recruitment accompanied by angiogenesis	1753:1802	Compared to PRP alone, chitosan-PRP hybrids had much greater capacity to induce local cell recruitment accompanied by angiogenesis, suggesting a strong potential for their use in regenerative medicine.
28084000	3	62	theme	chitosan	854:861	arg1	concentration					909:921	lyoprotectant concentration	895:921	lyoprotectant concentration	895:921	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	3	62	theme	chitosan	854:861	arg1	Mn					863:864	distinct chitosan Mn	845:864	distinct chitosan Mn	845:864	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	3	62	theme	chitosan	854:861	arg1	concentration					877:889	chitosan concentration	868:889	chitosan concentration	868:889	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	1	63	theme	freeze-dried	214:225	arg1	formulations					236:247	freeze-dried chitosan formulations	214:247	freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair	214:347	The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair.
28084000	6	64	theme	chitosan-PRP	1320:1331	arg1	hybrids					1333:1339	solid chitosan-PRP hybrids	1314:1339	solid chitosan-PRP hybrids	1314:1339	The paste-like chitosan-PRP mixtures coagulated quickly to form solid chitosan-PRP hybrids, which retracted much less than PRP-only controls.
28084000	7	65	theme	Homogeneous	1392:1402	arg1	dispersion					1404:1413	Homogeneous dispersion	1392:1413	Homogeneous dispersion of chitosan within the hybrid clots	1392:1449	Homogeneous dispersion of chitosan within the hybrid clots was strongly dependent on chitosan Mn , and occurred only with medium Mn chitosan.
28084000	2	66	theme	solid	719:723	arg1	implants					745:752	solid chitosan-PRP hybrid implants	719:752	solid chitosan-PRP hybrid implants that are stable and homogenous	719:783	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	67	theme	systematic	352:361	arg1	approach					363:370	A systematic approach	350:370	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration,	350:512	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	68	dep	coagulate	683:691	arg1	5 min					704:708	< 5 min	702:708	< 5 min	702:708	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	1	69	theme	chitosan	227:234	arg1	formulations					236:247	freeze-dried chitosan formulations	214:247	freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair	214:347	The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair.
28084000	0	70	theme	in	82:83	arg1	properties					91:100	in vitro properties	82:100	in vitro properties	82:100	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	0	70	theme	in	82:83	arg1	vascularization					156:170	vascularization	156:170	vascularization	156:170	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	0	70	theme	in	82:83	arg1	degradation					122:132	degradation	122:132	degradation	122:132	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	0	70	theme	in	82:83	arg1	recruitment					140:150	cell recruitment	135:150	cell recruitment	135:150	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	0	70	theme	in	82:83	arg1	residence					111:119	residence	111:119	residence	111:119	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	4	71	used	used	947:950	arg2	PRP					939:941	PRP	939:941	PRP	939:941	PRP was used to solubilize the freeze-dried cakes and assess in vitro and in vivo performance, the latter as dorsal subcutaneous injections into New Zealand White rabbits.
28084000	3	72	contain	containing	805:814	arg1	cakes					799:803	Freeze-dried cakes	786:803	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration,	786:922	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	3	72	contain	containing	805:814	arg2	concentration					877:889	chitosan concentration	868:889	chitosan concentration	868:889	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	3	72	contain	containing	805:814	arg2	chloride					824:831	calcium chloride	816:831	calcium chloride	816:831	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	3	72	contain	containing	805:814	arg2	Mn					863:864	distinct chitosan Mn	845:864	distinct chitosan Mn	845:864	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	3	72	contain	containing	805:814	arg2	concentration					909:921	lyoprotectant concentration	895:921	lyoprotectant concentration	895:921	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	9	73	contain	had	1717:1719	arg1	hybrids					1709:1715	chitosan-PRP hybrids	1696:1715	chitosan-PRP hybrids	1696:1715	Compared to PRP alone, chitosan-PRP hybrids had much greater capacity to induce local cell recruitment accompanied by angiogenesis, suggesting a strong potential for their use in regenerative medicine.
28084000	9	73	contain	had	1717:1719	arg2	capacity					1734:1741	much greater capacity	1721:1741	much greater capacity to induce local cell recruitment accompanied by angiogenesis	1721:1802	Compared to PRP alone, chitosan-PRP hybrids had much greater capacity to induce local cell recruitment accompanied by angiogenesis, suggesting a strong potential for their use in regenerative medicine.
28084000	4	74	theme	subcutaneous	1055:1066	arg1	injections					1068:1077	dorsal subcutaneous injections	1048:1077	dorsal subcutaneous injections into New Zealand White rabbits	1048:1108	PRP was used to solubilize the freeze-dried cakes and assess in vitro and in vivo performance, the latter as dorsal subcutaneous injections into New Zealand White rabbits.
28084000	2	75	theme	lyoprotectant	485:497	arg1	concentration					499:511	lyoprotectant concentration	485:511	lyoprotectant concentration	485:511	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	5	76	theme	Freeze-dried	1111:1122	arg1	formulations					1132:1143	Freeze-dried polymer formulations	1111:1143	Freeze-dried polymer formulations containing low and medium chitosan Mn and concentrations	1111:1200	Freeze-dried polymer formulations containing low and medium chitosan Mn and concentrations were rapidly and completely solubilized in PRP.
28084000	2	77	theme	average	432:438	arg1	mass					446:449	average molar mass	432:449	chitosan number average molar mass (Mn )	416:455	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	2	77	theme	average	432:438	arg1	Mn					452:453	Mn	452:453	Mn	452:453	A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration, was undertaken to identify compositions that would rapidly (< 1 min) and completely solubilize in PRP, would have paste-like handling properties upon solubilization and coagulate rapidly (< 5 min) to form solid chitosan-PRP hybrid implants that are stable and homogenous.
28084000	0	78	theme	chitosan-platelet-rich	11:32	arg1	implants					41:48	Injectable chitosan-platelet-rich plasma implants	0:48	Injectable chitosan-platelet-rich plasma implants	0:48	Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.
28084000	3	79	theme	chitosan	868:875	arg1	Mn					863:864	distinct chitosan Mn	845:864	distinct chitosan Mn	845:864	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	3	79	theme	chitosan	868:875	arg1	concentration					877:889	chitosan concentration	868:889	chitosan concentration	868:889	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28084000	3	80	theme	Freeze-dried	786:797	arg1	cakes					799:803	Freeze-dried cakes	786:803	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration,	786:922	Freeze-dried cakes containing calcium chloride, as well as distinct chitosan Mn , chitosan concentration and lyoprotectant concentration, were prepared.
28527999	2	0	theme	pentapeptide	447:458	arg1	[OGP					460:463	its C-terminal pentapeptide [OGP	432:463	its C-terminal pentapeptide [OGP(10-14)	432:470	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	2	0	theme	pentapeptide	447:458	arg1	10-14					465:469	10-14	465:469	10-14	465:469	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	8	1	theme	BC-COL	1123:1128	arg1	-Ap					1130:1132	the (BC-COL)-Ap	1118:1132	the (BC-COL)-Ap associated with OGP peptides	1118:1161	These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications.
28527999	6	2	theme	OGP-containing	969:982	arg1	BC-Ap					984:988	OGP-containing BC-Ap	969:988	OGP-containing BC-Ap	969:988	Additionally, the association among collagen, apatite, and OGP peptides enhanced cell growth compared with OGP-containing BC-Ap.
28527999	2	3	theme	C-terminal	436:445	arg1	[OGP					460:463	its C-terminal pentapeptide [OGP	432:463	its C-terminal pentapeptide [OGP(10-14)	432:470	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	2	3	theme	C-terminal	436:445	arg1	10-14					465:469	10-14	465:469	10-14	465:469	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	8	4	theme	bone	1209:1212	arg1	applications					1233:1244	bone tissue engineering applications	1209:1244	bone tissue engineering applications	1209:1244	These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications.
28527999	4	5	dep	in	679:680	arg1	situ					682:685	situ	682:685	situ	682:685	SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite.
28527999	1	6	theme	bone	156:159	arg1	repair/regeneration					161:179	bone repair/regeneration	156:179	bone repair/regeneration	156:179	Despite advances in the field of biomaterials for bone repair/regeneration, some challenges for developing an ideal bone substitute need to be overcome.
28527999	8	7	theme	engineering	1221:1231	arg1	applications					1233:1244	bone tissue engineering applications	1209:1244	bone tissue engineering applications	1209:1244	These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications.
28527999	4	8	theme	FTIR	639:642	arg1	analyses					656:663	SEM, FTIR and 31P NMR analyses	634:663	SEM, FTIR and 31P NMR analyses	634:663	SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite.
28527999	8	9	theme	potential	1185:1193	arg1	candidate					1195:1203	a potential candidate	1183:1203	a potential candidate for bone tissue engineering applications	1183:1244	These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications.
28527999	8	10	theme	OGP	1150:1152	arg1	peptides					1154:1161	OGP peptides	1150:1161	OGP peptides	1150:1161	These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications.
28527999	5	11	theme	OGP-containing	771:784	arg1	-Ap					794:796	The OGP-containing (BC-COL)-Ap	767:796	The OGP-containing (BC-COL)-Ap	767:796	The OGP-containing (BC-COL)-Ap stimulated the early development of the osteoblastic phenotype.
28527999	5	11	theme	OGP-containing	771:784	arg1	BC-COL					787:792	BC-COL	787:792	BC-COL	787:792	The OGP-containing (BC-COL)-Ap stimulated the early development of the osteoblastic phenotype.
28527999	6	12	theme	OGP	921:923	arg1	peptides					925:932	OGP peptides	921:932	OGP peptides	921:932	Additionally, the association among collagen, apatite, and OGP peptides enhanced cell growth compared with OGP-containing BC-Ap.
28527999	8	13	theme	tissue	1214:1219	arg1	applications					1233:1244	bone tissue engineering applications	1209:1244	bone tissue engineering applications	1209:1244	These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications.
28527999	4	14	theme	31P	648:650	arg1	NMR					652:654	31P NMR	648:654	31P NMR	648:654	SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite.
28527999	0	15	theme	Nanocellulose-collagen-apatite	0:29	arg1	composite					31:39	Nanocellulose-collagen-apatite composite	0:39	Nanocellulose-collagen-apatite composite	0:39	Nanocellulose-collagen-apatite composite associated with osteogenic growth peptide for bone regeneration.
28527999	6	16	theme	cell	943:946	arg1	growth					948:953	cell growth	943:953	cell growth	943:953	Additionally, the association among collagen, apatite, and OGP peptides enhanced cell growth compared with OGP-containing BC-Ap.
28527999	7	17	theme	mutagenic	1062:1070	arg1	effects					1072:1078	cytotoxic, genotoxic or mutagenic effects	1038:1078	cytotoxic, genotoxic or mutagenic effects	1038:1078	Furthermore, none of the nanocomposites showed cytotoxic, genotoxic or mutagenic effects.
28527999	1	18	theme	ideal	216:220	arg1	bone					222:225	an ideal bone substitute	213:236	an ideal bone substitute	213:236	Despite advances in the field of biomaterials for bone repair/regeneration, some challenges for developing an ideal bone substitute need to be overcome.
28527999	2	19	theme	growth	408:413	arg1	peptide					415:421	osteogenic growth peptide	397:421	osteogenic growth peptide (OGP)	397:427	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	2	19	theme	growth	408:413	arg1	OGP					424:426	OGP	424:426	OGP	424:426	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	4	20	theme	effective	715:723	arg1	synthesis					687:695	in situ synthesis	679:695	in situ synthesis to apatite	679:706	SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite.
28527999	4	20	theme	effective	715:723	arg1	route					725:729	an effective route	712:729	an effective route for obtaining of bone-like apatite	712:764	SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite.
28527999	2	21	theme	osteogenic	397:406	arg1	peptide					415:421	osteogenic growth peptide	397:421	osteogenic growth peptide (OGP)	397:427	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	2	21	theme	osteogenic	397:406	arg1	OGP					424:426	OGP	424:426	OGP	424:426	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	7	22	theme	genotoxic	1049:1057	arg1	effects					1072:1078	cytotoxic, genotoxic or mutagenic effects	1038:1078	cytotoxic, genotoxic or mutagenic effects	1038:1078	Furthermore, none of the nanocomposites showed cytotoxic, genotoxic or mutagenic effects.
28527999	3	23	theme	BC-COL	509:514	arg1	nanocomposites					516:529	The BC-COL nanocomposites	505:529	The BC-COL nanocomposites	505:529	The BC-COL nanocomposites were successfully obtained by carbodiimide-mediated coupling as demonstrated by spectroscopy analysis.
28527999	0	24	theme	growth	68:73	arg1	peptide					75:81	osteogenic growth peptide	57:81	osteogenic growth peptide for bone regeneration	57:103	Nanocellulose-collagen-apatite composite associated with osteogenic growth peptide for bone regeneration.
28527999	4	25	theme	in	679:680	arg1	synthesis					687:695	in situ synthesis	679:695	in situ synthesis to apatite	679:706	SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite.
28527999	4	25	theme	in	679:680	arg1	route					725:729	an effective route	712:729	an effective route for obtaining of bone-like apatite	712:764	SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite.
28527999	2	26	theme	regeneration	482:493	arg1	purposes					495:502	bone regeneration purposes	477:502	bone regeneration purposes	477:502	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	5	27	theme	osteoblastic	838:849	arg1	phenotype					851:859	the osteoblastic phenotype	834:859	the osteoblastic phenotype	834:859	The OGP-containing (BC-COL)-Ap stimulated the early development of the osteoblastic phenotype.
28527999	4	28	theme	SEM	634:636	arg1	analyses					656:663	SEM, FTIR and 31P NMR analyses	634:663	SEM, FTIR and 31P NMR analyses	634:663	SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite.
28527999	0	29	theme	osteogenic	57:66	arg1	peptide					75:81	osteogenic growth peptide	57:81	osteogenic growth peptide for bone regeneration	57:103	Nanocellulose-collagen-apatite composite associated with osteogenic growth peptide for bone regeneration.
28527999	2	30	theme	bone	477:480	arg1	purposes					495:502	bone regeneration purposes	477:502	bone regeneration purposes	477:502	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	3	31	theme	spectroscopy	611:622	arg1	analysis					624:631	spectroscopy analysis	611:631	spectroscopy analysis	611:631	The BC-COL nanocomposites were successfully obtained by carbodiimide-mediated coupling as demonstrated by spectroscopy analysis.
28527999	3	32	theme	carbodiimide-mediated	561:581	arg1	coupling					583:590	carbodiimide-mediated coupling	561:590	carbodiimide-mediated coupling	561:590	The BC-COL nanocomposites were successfully obtained by carbodiimide-mediated coupling as demonstrated by spectroscopy analysis.
28527999	5	33	theme	early	813:817	arg1	development					819:829	the early development	809:829	the early development of the osteoblastic phenotype	809:859	The OGP-containing (BC-COL)-Ap stimulated the early development of the osteoblastic phenotype.
28527999	8	34	theme	promising	1087:1095	arg1	results					1097:1103	These promising results	1081:1103	These promising results	1081:1103	These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications.
28527999	2	35	dep	nanocomposite	315:327	arg1	vitro					307:311	vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP)	307:427	vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP)	307:427	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	7	36	theme	cytotoxic	1038:1046	arg1	effects					1072:1078	cytotoxic, genotoxic or mutagenic effects	1038:1078	cytotoxic, genotoxic or mutagenic effects	1038:1078	Furthermore, none of the nanocomposites showed cytotoxic, genotoxic or mutagenic effects.
28527999	7	37	theme	nanocomposites	1016:1029	arg1	none					1004:1007	none	1004:1007	none of the nanocomposites	1004:1029	Furthermore, none of the nanocomposites showed cytotoxic, genotoxic or mutagenic effects.
28527999	1	38	theme	biomaterials	139:150	arg1	field					130:134	the field	126:134	the field of biomaterials for bone repair/regeneration	126:179	Despite advances in the field of biomaterials for bone repair/regeneration, some challenges for developing an ideal bone substitute need to be overcome.
28527999	4	39	theme	bone-like	748:756	arg1	apatite					758:764	bone-like apatite	748:764	bone-like apatite	748:764	SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite.
28527999	2	40	theme	bacterial	338:346	arg1	BC					359:360	BC	359:360	BC	359:360	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	2	40	theme	bacterial	338:346	arg1	cellulose					348:356	bacterial cellulose	338:356	bacterial cellulose (BC)	338:361	Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes.
28527999	1	41	from	advances	114:121	arg1	field					130:134	the field	126:134	the field of biomaterials for bone repair/regeneration	126:179	Despite advances in the field of biomaterials for bone repair/regeneration, some challenges for developing an ideal bone substitute need to be overcome.
28527999	1	42	dep	bone	222:225	arg1	substitute					227:236	substitute	227:236	substitute	227:236	Despite advances in the field of biomaterials for bone repair/regeneration, some challenges for developing an ideal bone substitute need to be overcome.
28527999	5	43	theme	phenotype	851:859	arg1	development					819:829	the early development	809:829	the early development of the osteoblastic phenotype	809:859	The OGP-containing (BC-COL)-Ap stimulated the early development of the osteoblastic phenotype.
28527999	0	44	theme	bone	87:90	arg1	regeneration					92:103	bone regeneration	87:103	bone regeneration	87:103	Nanocellulose-collagen-apatite composite associated with osteogenic growth peptide for bone regeneration.
28527999	4	45	theme	NMR	652:654	arg1	analyses					656:663	SEM, FTIR and 31P NMR analyses	634:663	SEM, FTIR and 31P NMR analyses	634:663	SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite.
24927660	3	0	theme	modern	321:326	arg1	possibilities					328:340	modern possibilities	321:340	modern possibilities of morphological imaging	321:365	The first part therefore deals with modern possibilities of morphological imaging.
24927660	5	1	theme	transfer	820:827	arg1	technique					839:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	1	2	theme	pathologies	156:166	arg1	detection					126:134	the detection	122:134	the detection of subtle cartilage pathologies	122:166	Excellent morphological imaging of cartilage is now possible and allows the detection of subtle cartilage pathologies.
24927660	5	3	theme	delayed	627:633	arg1	imaging					674:680	delayed gadolinium-enhanced magnetic resonance imaging	627:680	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	627:703	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	3	theme	delayed	627:633	arg1	dGEMRIC					696:702	dGEMRIC	696:702	dGEMRIC	696:702	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	4	4	theme	cartilage	406:414	arg1	imaging					416:422	functional cartilage imaging	395:422	functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions	395:595	The second part deals with functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions.
24927660	1	5	theme	morphological	60:72	arg1	imaging					74:80	Excellent morphological imaging	50:80	Excellent morphological imaging of cartilage	50:93	Excellent morphological imaging of cartilage is now possible and allows the detection of subtle cartilage pathologies.
24927660	4	6	dep	changes	460:466	arg1	osteoarthritis					508:521	early osteoarthritis	502:521	early osteoarthritis	502:521	The second part deals with functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions.
24927660	5	7	theme	T2	709:710	arg1	mapping					712:718	T2 mapping	709:718	T2 mapping	709:718	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	8	theme	magnetic	655:662	arg1	resonance					664:672	gadolinium-enhanced magnetic resonance	635:672	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	627:703	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	3	9	theme	imaging	359:365	arg1	possibilities					328:340	modern possibilities	321:340	modern possibilities of morphological imaging	321:365	The first part therefore deals with modern possibilities of morphological imaging.
24927660	5	10	theme	resonance	664:672	arg1	imaging					674:680	delayed gadolinium-enhanced magnetic resonance imaging	627:680	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	627:703	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	10	theme	resonance	664:672	arg1	dGEMRIC					696:702	dGEMRIC	696:702	dGEMRIC	696:702	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	11	theme	chemical	781:788	arg1	technique					839:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	4	12	theme	early	502:506	arg1	osteoarthritis					508:521	early osteoarthritis	502:521	early osteoarthritis	502:521	The second part deals with functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions.
24927660	5	13	theme	Validated	598:606	arg1	technique					839:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	13	theme	Validated	598:606	arg1	mapping					712:718	T2 mapping	709:718	T2 mapping	709:718	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	13	theme	Validated	598:606	arg1	techniques					608:617	Validated techniques	598:617	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	598:847	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	13	theme	Validated	598:606	arg1	imaging					674:680	delayed gadolinium-enhanced magnetic resonance imaging	627:680	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	627:703	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	1	14	theme	cartilage	85:93	arg1	imaging					74:80	Excellent morphological imaging	50:80	Excellent morphological imaging of cartilage	50:93	Excellent morphological imaging of cartilage is now possible and allows the detection of subtle cartilage pathologies.
24927660	5	15	theme	glycosaminoglycan	763:779	arg1	technique					839:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	16	theme	cartilage	685:693	arg1	imaging					674:680	delayed gadolinium-enhanced magnetic resonance imaging	627:680	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	627:703	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	16	theme	cartilage	685:693	arg1	mapping					712:718	T2 mapping	709:718	T2 mapping	709:718	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	16	theme	cartilage	685:693	arg1	dGEMRIC					696:702	dGEMRIC	696:702	dGEMRIC	696:702	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	4	17	theme	therapeutic	571:581	arg1	interventions					583:595	therapeutic interventions	571:595	therapeutic interventions	571:595	The second part deals with functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions.
24927660	4	18	theme	functional	395:404	arg1	imaging					416:422	functional cartilage imaging	395:422	functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions	395:595	The second part deals with functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions.
24927660	0	19	theme	cartilage	30:38	arg1	imaging					40:46	functional cartilage imaging	19:46	functional cartilage imaging	19:46	[Morphological and functional cartilage imaging].
24927660	5	20	theme	latest	732:737	arg1	technique					839:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	20	theme	latest	732:737	arg1	techniques					739:748	the latest techniques	728:748	the latest techniques	728:748	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	20	theme	latest	732:737	arg1	mapping					712:718	T2 mapping	709:718	T2 mapping	709:718	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	5	20	theme	latest	732:737	arg1	imaging					674:680	delayed gadolinium-enhanced magnetic resonance imaging	627:680	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	627:703	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	0	21	theme	functional	19:28	arg1	imaging					40:46	functional cartilage imaging	19:46	functional cartilage imaging	19:46	[Morphological and functional cartilage imaging].
24927660	3	22	theme	first	289:293	arg1	part					295:298	The first part	285:298	The first part	285:298	The first part therefore deals with modern possibilities of morphological imaging.
24927660	2	23	theme	2D	190:191	arg1	sequences					193:201	the standard 2D sequences	177:201	the standard 2D sequences	177:201	Besides the standard 2D sequences, a multitude of 3D sequences are available for high-resolution cartilage imaging.
24927660	4	24	with	possible	441:448	arg1	imaging					416:422	functional cartilage imaging	395:422	functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions	395:595	The second part deals with functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions.
24927660	5	25	theme	gagCEST	830:836	arg1	technique					839:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	4	26	from	changes	460:466	arg1	composition					481:491	cartilage composition	471:491	cartilage composition	471:491	The second part deals with functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions.
24927660	2	27	theme	standard	181:188	arg1	sequences					193:201	the standard 2D sequences	177:201	the standard 2D sequences	177:201	Besides the standard 2D sequences, a multitude of 3D sequences are available for high-resolution cartilage imaging.
24927660	4	28	theme	cartilage	471:479	arg1	composition					481:491	cartilage composition	471:491	cartilage composition	471:491	The second part deals with functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions.
24927660	2	29	theme	sequences	222:230	arg1	available					236:244	available	236:244	available	236:244	Besides the standard 2D sequences, a multitude of 3D sequences are available for high-resolution cartilage imaging.
24927660	2	29	theme	sequences	222:230	arg1	multitude					206:214	a multitude	204:214	a multitude of 3D sequences	204:230	Besides the standard 2D sequences, a multitude of 3D sequences are available for high-resolution cartilage imaging.
24927660	5	30	theme	saturation	809:818	arg1	technique					839:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	2	31	theme	3D	219:220	arg1	sequences					222:230	3D sequences	219:230	3D sequences	219:230	Besides the standard 2D sequences, a multitude of 3D sequences are available for high-resolution cartilage imaging.
24927660	4	32	theme	second	372:377	arg1	part					379:382	The second part	368:382	The second part	368:382	The second part deals with functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions.
24927660	4	33	theme	biochemical	545:555	arg1	changes					557:563	biochemical changes	545:563	biochemical changes after therapeutic interventions	545:595	The second part deals with functional cartilage imaging with which it is possible to detect changes in cartilage composition and thus early osteoarthritis as well as to monitor biochemical changes after therapeutic interventions.
24927660	2	34	theme	cartilage	266:274	arg1	imaging					276:282	high-resolution cartilage imaging	250:282	high-resolution cartilage imaging	250:282	Besides the standard 2D sequences, a multitude of 3D sequences are available for high-resolution cartilage imaging.
24927660	5	35	theme	gadolinium-enhanced	635:653	arg1	resonance					664:672	gadolinium-enhanced magnetic resonance	635:672	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	627:703	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	2	36	theme	high-resolution	250:264	arg1	imaging					276:282	high-resolution cartilage imaging	250:282	high-resolution cartilage imaging	250:282	Besides the standard 2D sequences, a multitude of 3D sequences are available for high-resolution cartilage imaging.
24927660	5	37	theme	exchange-dependent	790:807	arg1	technique					839:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique	759:847	Validated techniques such as delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping as well the latest techniques, such as the glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) technique will be discussed.
24927660	1	38	theme	subtle	139:144	arg1	pathologies					156:166	subtle cartilage pathologies	139:166	subtle cartilage pathologies	139:166	Excellent morphological imaging of cartilage is now possible and allows the detection of subtle cartilage pathologies.
24927660	3	39	theme	morphological	345:357	arg1	imaging					359:365	morphological imaging	345:365	morphological imaging	345:365	The first part therefore deals with modern possibilities of morphological imaging.
24927660	1	40	theme	Excellent	50:58	arg1	imaging					74:80	Excellent morphological imaging	50:80	Excellent morphological imaging of cartilage	50:93	Excellent morphological imaging of cartilage is now possible and allows the detection of subtle cartilage pathologies.
24927660	1	41	theme	cartilage	146:154	arg1	pathologies					156:166	subtle cartilage pathologies	139:166	subtle cartilage pathologies	139:166	Excellent morphological imaging of cartilage is now possible and allows the detection of subtle cartilage pathologies.
25752097	0	0	theme	enantiomers	80:90	arg1	determination					57:69	determination	57:69	determination of their enantiomers	57:90	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	0	0	theme	enantiomers	80:90	arg1	separation					9:18	[[Chiral separation	0:18	[[Chiral separation of five arylpropionic acid drugs	0:51	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	3	1	dep	%	788:788	arg1	v/v					791:793	v/v	791:793	v/v	791:793	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
25752097	3	2	theme	%	788:788	arg1	acid					803:806	methanol-0.1% (v/v) formic acid	776:806	methanol-0.1% (v/v) formic acid except naproxen	776:822	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
25752097	2	3	theme	flurbiprofen	601:612	arg1	separation					587:596	chiral separation	580:596	chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen	580:661	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	1	4	theme	arylpropionic	296:308	arg1	drugs					315:319	arylpropionic acid drugs	296:319	arylpropionic acid drugs	296:319	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	0	5	from	separation	9:18	arg1	preparations					110:121	pharmaceutical preparations	95:121	pharmaceutical preparations	95:121	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	0	6	theme	4-methylbenzoate	201:216	arg1	phase					230:234	cellulose-tris-(4-methylbenzoate) stationary phase]]	185:236	cellulose-tris-(4-methylbenzoate) stationary phase]]	185:236	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	2	7	theme	naproxen	628:635	arg1	separation					587:596	chiral separation	580:596	chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen	580:661	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	1	8	theme	acid	310:313	arg1	drugs					315:319	arylpropionic acid drugs	296:319	arylpropionic acid drugs	296:319	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	0	9	theme	pharmaceutical	95:108	arg1	preparations					110:121	pharmaceutical preparations	95:121	pharmaceutical preparations	95:121	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	3	10	theme	acid	803:806	arg1	phase					767:771	the mobile phase	756:771	the mobile phase of methanol-0.1% (v/v) formic acid except naproxen	756:822	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
25752097	0	11	with	chromatography	165:178	arg1	phase					230:234	cellulose-tris-(4-methylbenzoate) stationary phase]]	185:236	cellulose-tris-(4-methylbenzoate) stationary phase]]	185:236	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	1	12	theme	drugs	315:319	arg1	separation					282:291	enantiomeric separation	269:291	enantiomeric separation of arylpropionic acid drugs	269:319	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	5	13	theme	chromatographic	1124:1138	arg1	behaviors					1140:1148	their chromatographic behaviors	1118:1148	their chromatographic behaviors	1118:1148	The resolutions between respective enantiomers for arylpropionic acid drugs on CTMB had significant differences due to their chromatographic behaviors.
25752097	0	14	theme	stationary	219:228	arg1	phase					230:234	cellulose-tris-(4-methylbenzoate) stationary phase]]	185:236	cellulose-tris-(4-methylbenzoate) stationary phase]]	185:236	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	4	15	theme	resolution	903:912	arg1	mechanisms					877:886	The mechanisms	873:886	The mechanisms of the racemic resolution for the above mentioned five drugs	873:947	The mechanisms of the racemic resolution for the above mentioned five drugs are discussed thermodynamically and structurally.
25752097	2	16	theme	additives	551:559	arg1	composition					518:528	the composition	514:528	the composition of the mobile phase, additives and temperature	514:575	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	2	17	from	effects	503:509	arg1	separation					587:596	chiral separation	580:596	chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen	580:661	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	7	18	from	preparations	1371:1382	arg1	determination					1306:1318	the determination	1302:1318	the determination of the enantiomers of the five drugs in commercial preparations under the optimized conditions	1302:1413	The method established has been successfully applied to the determination of the enantiomers of the five drugs in commercial preparations under the optimized conditions.
25752097	2	19	theme	composition	518:528	arg1	effects					503:509	The effects	499:509	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen	499:661	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	3	20	theme	%	843:843	arg1	acid					858:861	acetonitrile-0.1% (v/v) formic acid	827:861	acetonitrile-0.1% (v/v) formic acid at 25 °C	827:870	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
25752097	5	21	from	resolutions	1003:1013	arg1	CTMB					1078:1081	CTMB	1078:1081	CTMB	1078:1081	The resolutions between respective enantiomers for arylpropionic acid drugs on CTMB had significant differences due to their chromatographic behaviors.
25752097	5	22	contain	had	1083:1085	arg1	resolutions					1003:1013	The resolutions	999:1013	The resolutions between respective enantiomers for arylpropionic acid drugs on CTMB	999:1081	The resolutions between respective enantiomers for arylpropionic acid drugs on CTMB had significant differences due to their chromatographic behaviors.
25752097	5	22	contain	had	1083:1085	arg2	differences					1099:1109	significant differences	1087:1109	significant differences	1087:1109	The resolutions between respective enantiomers for arylpropionic acid drugs on CTMB had significant differences due to their chromatographic behaviors.
25752097	3	23	theme	mobile	760:765	arg1	phase					767:771	the mobile phase	756:771	the mobile phase of methanol-0.1% (v/v) formic acid except naproxen	756:822	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
25752097	3	24	theme	formic	851:856	arg1	acid					858:861	acetonitrile-0.1% (v/v) formic acid	827:861	acetonitrile-0.1% (v/v) formic acid at 25 °C	827:870	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
25752097	7	25	theme	commercial	1360:1369	arg1	preparations					1371:1382	commercial preparations	1360:1382	commercial preparations under the optimized conditions	1360:1413	The method established has been successfully applied to the determination of the enantiomers of the five drugs in commercial preparations under the optimized conditions.
25752097	0	26	theme	[[Chiral	0:7	arg1	separation					9:18	[[Chiral separation	0:18	[[Chiral separation of five arylpropionic acid drugs	0:51	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	2	27	theme	loxoprofen	652:661	arg1	separation					587:596	chiral separation	580:596	chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen	580:661	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	7	28	from	enantiomers	1327:1337	arg1	preparations					1371:1382	commercial preparations	1360:1382	commercial preparations under the optimized conditions	1360:1413	The method established has been successfully applied to the determination of the enantiomers of the five drugs in commercial preparations under the optimized conditions.
25752097	2	29	theme	ibuprofen	638:646	arg1	separation					587:596	chiral separation	580:596	chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen	580:661	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	2	30	theme	chiral	580:585	arg1	separation					587:596	chiral separation	580:596	chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen	580:661	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	7	31	theme	optimized	1394:1402	arg1	conditions					1404:1413	the optimized conditions	1390:1413	the optimized conditions	1390:1413	The method established has been successfully applied to the determination of the enantiomers of the five drugs in commercial preparations under the optimized conditions.
25752097	0	32	theme	high	141:144	arg1	chromatography					165:178	reversed-phase high performance liquid chromatography	126:178	reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]]	126:236	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	7	33	from	determination	1306:1318	arg1	preparations					1371:1382	commercial preparations	1360:1382	commercial preparations under the optimized conditions	1360:1413	The method established has been successfully applied to the determination of the enantiomers of the five drugs in commercial preparations under the optimized conditions.
25752097	2	34	theme	temperature	565:575	arg1	composition					518:528	the composition	514:528	the composition of the mobile phase, additives and temperature	514:575	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	5	35	theme	significant	1087:1097	arg1	differences					1099:1109	significant differences	1087:1109	significant differences	1087:1109	The resolutions between respective enantiomers for arylpropionic acid drugs on CTMB had significant differences due to their chromatographic behaviors.
25752097	3	36	theme	methanol-0.1	776:787	arg1	%					788:788	%	788:788	%	788:788	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
25752097	0	37	theme	reversed-phase	126:139	arg1	chromatography					165:178	reversed-phase high performance liquid chromatography	126:178	reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]]	126:236	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	3	38	dep	%	843:843	arg1	v/v					846:848	v/v	846:848	v/v	846:848	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
25752097	3	39	from	°C	869:870	arg1	acid					858:861	acetonitrile-0.1% (v/v) formic acid	827:861	acetonitrile-0.1% (v/v) formic acid at 25 °C	827:870	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
25752097	0	40	theme	acid	42:45	arg1	drugs					47:51	five arylpropionic acid drugs	23:51	five arylpropionic acid drugs	23:51	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	1	41	theme	reversed	354:361	arg1	RP-HPLC					409:415	RP-HPLC	409:415	RP-HPLC	409:415	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	1	41	theme	reversed	354:361	arg1	chromatography					393:406	reversed phase-high performance liquid chromatography	354:406	reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP)	354:496	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	0	42	theme	liquid	158:163	arg1	chromatography					165:178	reversed-phase high performance liquid chromatography	126:178	reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]]	126:236	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	4	43	theme	mentioned	928:936	arg1	drugs					943:947	the above mentioned five drugs	918:947	the above mentioned five drugs	918:947	The mechanisms of the racemic resolution for the above mentioned five drugs are discussed thermodynamically and structurally.
25752097	1	44	theme	Chromatographic	239:253	arg1	behaviors					255:263	Chromatographic behaviors	239:263	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs	239:319	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	0	45	theme	arylpropionic	28:40	arg1	drugs					47:51	five arylpropionic acid drugs	23:51	five arylpropionic acid drugs	23:51	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	1	46	theme	phase-high	363:372	arg1	RP-HPLC					409:415	RP-HPLC	409:415	RP-HPLC	409:415	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	1	46	theme	phase-high	363:372	arg1	chromatography					393:406	reversed phase-high performance liquid chromatography	354:406	reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP)	354:496	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	0	47	theme	performance	146:156	arg1	chromatography					165:178	reversed-phase high performance liquid chromatography	126:178	reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]]	126:236	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	5	48	theme	arylpropionic	1050:1062	arg1	drugs					1069:1073	arylpropionic acid drugs	1050:1073	arylpropionic acid drugs	1050:1073	The resolutions between respective enantiomers for arylpropionic acid drugs on CTMB had significant differences due to their chromatographic behaviors.
25752097	1	49	theme	performance	374:384	arg1	RP-HPLC					409:415	RP-HPLC	409:415	RP-HPLC	409:415	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	1	49	theme	performance	374:384	arg1	chromatography					393:406	reversed phase-high performance liquid chromatography	354:406	reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP)	354:496	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	4	50	theme	racemic	895:901	arg1	resolution					903:912	the racemic resolution	891:912	the racemic resolution for the above mentioned five drugs	891:947	The mechanisms of the racemic resolution for the above mentioned five drugs are discussed thermodynamically and structurally.
25752097	0	51	from	determination	57:69	arg1	preparations					110:121	pharmaceutical preparations	95:121	pharmaceutical preparations	95:121	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	5	52	theme	acid	1064:1067	arg1	drugs					1069:1073	arylpropionic acid drugs	1050:1073	arylpropionic acid drugs	1050:1073	The resolutions between respective enantiomers for arylpropionic acid drugs on CTMB had significant differences due to their chromatographic behaviors.
25752097	7	53	theme	enantiomers	1327:1337	arg1	determination					1306:1318	the determination	1302:1318	the determination of the enantiomers of the five drugs in commercial preparations under the optimized conditions	1302:1413	The method established has been successfully applied to the determination of the enantiomers of the five drugs in commercial preparations under the optimized conditions.
25752097	0	54	theme	drugs	47:51	arg1	determination					57:69	determination	57:69	determination of their enantiomers	57:90	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	0	54	theme	drugs	47:51	arg1	separation					9:18	[[Chiral separation	0:18	[[Chiral separation of five arylpropionic acid drugs	0:51	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	1	55	theme	liquid	386:391	arg1	RP-HPLC					409:415	RP-HPLC	409:415	RP-HPLC	409:415	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	1	55	theme	liquid	386:391	arg1	chromatography					393:406	reversed phase-high performance liquid chromatography	354:406	reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP)	354:496	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	2	56	theme	pranoprofen	615:625	arg1	separation					587:596	chiral separation	580:596	chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen	580:661	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	1	57	theme	chiral	468:473	arg1	CSP					493:495	CSP	493:495	CSP	493:495	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	1	57	theme	chiral	468:473	arg1	phase					486:490	chiral stationary phase	468:490	chiral stationary phase (CSP)	468:496	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	6	58	theme	resolutions	1164:1174	arg1	order					1155:1159	The order	1151:1159	The order of resolutions	1151:1174	The order of resolutions ranked pranoprofen, loxoprofen, flurbiprofen, ibuprofen and naproxen.
25752097	3	59	theme	formic	796:801	arg1	acid					803:806	methanol-0.1% (v/v) formic acid	776:806	methanol-0.1% (v/v) formic acid except naproxen	776:822	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
25752097	1	60	theme	stationary	475:484	arg1	CSP					493:495	CSP	493:495	CSP	493:495	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	1	60	theme	stationary	475:484	arg1	phase					486:490	chiral stationary phase	468:490	chiral stationary phase (CSP)	468:496	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	3	61	theme	acetonitrile-0.1	827:842	arg1	%					843:843	%	843:843	%	843:843	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
25752097	0	62	theme	cellulose-tris-	185:199	arg1	phase					230:234	cellulose-tris-(4-methylbenzoate) stationary phase]]	185:236	cellulose-tris-(4-methylbenzoate) stationary phase]]	185:236	[[Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris-(4-methylbenzoate) stationary phase]].
25752097	1	63	theme	enantiomeric	269:280	arg1	separation					282:291	enantiomeric separation	269:291	enantiomeric separation of arylpropionic acid drugs	269:319	Chromatographic behaviors for enantiomeric separation of arylpropionic acid drugs were systematically developed by reversed phase-high performance liquid chromatography (RP-HPLC) using cellulose-tris-(4-methylbenzoate) (CTMB) as chiral stationary phase (CSP).
25752097	2	64	theme	phase	544:548	arg1	composition					518:528	the composition	514:528	the composition of the mobile phase, additives and temperature	514:575	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	4	65	theme	above	922:926	arg1	drugs					943:947	the above mentioned five drugs	918:947	the above mentioned five drugs	918:947	The mechanisms of the racemic resolution for the above mentioned five drugs are discussed thermodynamically and structurally.
25752097	5	66	theme	respective	1023:1032	arg1	enantiomers					1034:1044	respective enantiomers	1023:1044	respective enantiomers for arylpropionic acid drugs	1023:1073	The resolutions between respective enantiomers for arylpropionic acid drugs on CTMB had significant differences due to their chromatographic behaviors.
25752097	7	67	theme	drugs	1351:1355	arg1	enantiomers					1327:1337	the enantiomers	1323:1337	the enantiomers of the five drugs in commercial preparations under the optimized conditions	1323:1413	The method established has been successfully applied to the determination of the enantiomers of the five drugs in commercial preparations under the optimized conditions.
25752097	2	68	theme	mobile	537:542	arg1	phase					544:548	the mobile phase	533:548	the mobile phase	533:548	The effects of the composition of the mobile phase, additives and temperature on chiral separation of flurbiprofen, pranoprofen, naproxen, ibuprofen and loxoprofen were further investigated.
25752097	3	69	theme	CTMB	748:751	arg1	CSP					741:743	CSP	741:743	CSP of CTMB	741:751	The enantiomers had been successfully separated on CSP of CTMB by the mobile phase of methanol-0.1% (v/v) formic acid except naproxen by acetonitrile-0.1% (v/v) formic acid at 25 °C.
24525904	9	0	from	involvement	1767:1777	arg1	biosynthesis					1904:1915	xylan backbone biosynthesis	1889:1915	xylan backbone biosynthesis	1889:1915	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	7	1	theme	Complementation	1192:1206	arg1	analysis					1208:1215	Complementation analysis	1192:1215	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants	1192:1278	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	6	2	located	localized	1135:1143	arg1	Golgi					1152:1156	the Golgi	1148:1156	the Golgi	1148:1156	OsGT43 proteins were shown to be localized in the Golgi, where xylan biosynthesis occurs.
24525904	6	2	located	localized	1135:1143	arg2	proteins					1109:1116	OsGT43 proteins	1102:1116	OsGT43 proteins	1102:1116	OsGT43 proteins were shown to be localized in the Golgi, where xylan biosynthesis occurs.
24525904	7	3	theme	stem	1440:1443	arg1	strength					1456:1463	defective stem mechanical strength	1430:1463	defective stem mechanical strength	1430:1463	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	5	4	from	activity	868:875	arg1	microsomes					890:899	rice stem microsomes	880:899	rice stem microsomes	880:899	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	3	5	theme	IRX9/IRX9	560:568	arg1	groups					552:557	2 functionally nonredundant groups	524:557	2 functionally nonredundant groups	524:557	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	3	5	theme	IRX9/IRX9	560:568	arg1	homolog					570:576	IRX9/IRX9 homolog	560:576	IRX9/IRX9 homolog	560:576	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	7	6	from	expression	1220:1229	arg1	irx9					1257:1260	Arabidopsis irx9	1245:1260	Arabidopsis irx9	1245:1260	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	7	6	from	expression	1220:1229	arg1	mutants					1272:1278	irx14 mutants	1266:1278	irx14 mutants	1266:1278	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	8	7	theme	other	1578:1582	arg1	hand					1584:1587	the other hand	1574:1587	the other hand	1574:1587	On the other hand, OsGT43J but not OsGT43A, OsGT43E, and OsGT43H restored the defective xylan phenotype in the irx14 mutant.
24525904	8	8	theme	defective	1649:1657	arg1	phenotype					1665:1673	the defective xylan phenotype	1645:1673	the defective xylan phenotype in the irx14 mutant	1645:1693	On the other hand, OsGT43J but not OsGT43A, OsGT43E, and OsGT43H restored the defective xylan phenotype in the irx14 mutant.
24525904	1	9	theme	rice	165:168	arg1	tissues					181:187	rice vegetative tissues	165:187	rice vegetative tissues	165:187	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	4	10	theme	family	689:694	arg1	members					701:707	family GT43 members	689:707	family GT43 members	689:707	The rice genome harbors 10 genes encoding family GT43 members and it is currently unknown whether they are all involved in xylan biosynthesis.
24525904	1	11	theme	tissues	181:187	arg1	walls					156:160	both primary and secondary cell walls	124:160	both primary and secondary cell walls of rice vegetative tissues	124:187	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	4	12	theme	rice	651:654	arg1	genome					656:661	The rice genome	647:661	The rice genome	647:661	The rice genome harbors 10 genes encoding family GT43 members and it is currently unknown whether they are all involved in xylan biosynthesis.
24525904	5	13	theme	xylan	843:847	arg1	activity					868:875	xylan xylosyltransferase activity	843:875	xylan xylosyltransferase activity in rice stem microsomes	843:899	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	14	theme	biochemical	819:829	arg1	analysis					831:838	biochemical analysis	819:838	biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes	819:899	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	15	theme	activity	868:875	arg1	analysis					831:838	biochemical analysis	819:838	biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes	819:899	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	9	16	theme	IRX14	1870:1874	arg1	homolog					1876:1882	an IRX14 homolog	1867:1882	an IRX14 homolog	1867:1882	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	9	16	theme	IRX14	1870:1874	arg1	OsGT43A					1818:1824	OsGT43A	1818:1824	OsGT43A	1818:1824	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	2	17	theme	biomass	240:246	arg1	recalcitrance					248:260	biomass recalcitrance	240:260	biomass recalcitrance	240:260	Since xylan is one of the factors contributing to biomass recalcitrance, understanding how xylan is synthesized in rice will potentially provide tools to modify grass biomass composition better suited for biofuel production.
24525904	5	18	theme	rice	948:951	arg1	OsGT43A					967:973	OsGT43A	967:973	OsGT43A	967:973	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	18	theme	rice	948:951	arg1	OsGT43H					1021:1027	OsGT43H	1021:1027	OsGT43H	1021:1027	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	18	theme	rice	948:951	arg1	OsGT43J					1052:1058	OsGT43J	1052:1058	OsGT43J	1052:1058	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	18	theme	rice	948:951	arg1	members					958:964	4 representative rice GT43 members	931:964	4 representative rice GT43 members	931:964	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	18	theme	rice	948:951	arg1	OsGT43E					994:1000	OsGT43E	994:1000	OsGT43E	994:1000	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	2	19	dep	understanding	263:275	arg1	synthesized					290:300	synthesized	290:300	is synthesized in rice	287:308	Since xylan is one of the factors contributing to biomass recalcitrance, understanding how xylan is synthesized in rice will potentially provide tools to modify grass biomass composition better suited for biofuel production.
24525904	0	20	theme	xylan	60:64	arg1	biosynthesis					66:77	xylan biosynthesis	60:77	xylan biosynthesis	60:77	Functional roles of rice glycosyltransferase family GT43 in xylan biosynthesis.
24525904	3	21	theme	backbone	626:633	arg1	elongation					635:644	xylan backbone elongation	620:644	xylan backbone elongation	620:644	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	1	22	theme	secondary	141:149	arg1	walls					156:160	both primary and secondary cell walls	124:160	both primary and secondary cell walls of rice vegetative tissues	124:187	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	9	23	theme	backbone	1895:1902	arg1	biosynthesis					1904:1915	xylan backbone biosynthesis	1889:1915	xylan backbone biosynthesis	1889:1915	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	7	24	theme	xylan	1518:1522	arg1	length					1530:1535	xylan chain length	1518:1535	xylan chain length	1518:1535	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	4	25	theme	xylan	770:774	arg1	biosynthesis					776:787	xylan biosynthesis	770:787	xylan biosynthesis	770:787	The rice genome harbors 10 genes encoding family GT43 members and it is currently unknown whether they are all involved in xylan biosynthesis.
24525904	1	26	theme	primary	129:135	arg1	walls					156:160	both primary and secondary cell walls	124:160	both primary and secondary cell walls of rice vegetative tissues	124:187	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	3	27	theme	biosynthesis	432:443	arg1	Studies					415:421	Studies	415:421	Studies of xylan biosynthesis in Arabidopsis	415:458	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	9	28	theme	rice	1728:1731	arg1	family					1738:1743	the rice GT43 family	1724:1743	the rice GT43 family	1724:1743	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	0	29	theme	Functional	0:9	arg1	roles					11:15	Functional roles	0:15	Functional roles of rice glycosyltransferase family GT43 in xylan biosynthesis	0:77	Functional roles of rice glycosyltransferase family GT43 in xylan biosynthesis.
24525904	1	30	from	present	113:119	arg1	walls					156:160	both primary and secondary cell walls	124:160	both primary and secondary cell walls of rice vegetative tissues	124:187	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	5	31	theme	members	958:964	arg1	roles					922:926	the roles	918:926	the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis	918:1099	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	0	32	theme	glycosyltransferase	25:43	arg1	family					45:50	rice glycosyltransferase family GT43	20:55	rice glycosyltransferase family GT43	20:55	Functional roles of rice glycosyltransferase family GT43 in xylan biosynthesis.
24525904	7	33	theme	xylan	1485:1489	arg1	content					1491:1497	xylan content	1485:1497	xylan content	1485:1497	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	7	34	theme	Arabidopsis	1245:1255	arg1	irx9					1257:1260	Arabidopsis irx9	1245:1260	Arabidopsis irx9	1245:1260	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	3	35	theme	family	479:484	arg1	glycosyltransferases					491:510	family GT43 glycosyltransferases	479:510	family GT43 glycosyltransferases	479:510	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	7	36	theme	OsGT43s	1234:1240	arg1	expression					1220:1229	expression	1220:1229	expression of OsGT43s in Arabidopsis irx9 and irx14 mutants	1220:1278	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	8	37	from	phenotype	1665:1673	arg1	mutant					1688:1693	the irx14 mutant	1678:1693	the irx14 mutant	1678:1693	On the other hand, OsGT43J but not OsGT43A, OsGT43E, and OsGT43H restored the defective xylan phenotype in the irx14 mutant.
24525904	2	38	theme	biomass	357:363	arg1	composition					365:375	grass biomass composition	351:375	grass biomass composition better suited for biofuel production	351:412	Since xylan is one of the factors contributing to biomass recalcitrance, understanding how xylan is synthesized in rice will potentially provide tools to modify grass biomass composition better suited for biofuel production.
24525904	5	39	theme	rice	880:883	arg1	microsomes					890:899	rice stem microsomes	880:899	rice stem microsomes	880:899	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	7	40	theme	mechanical	1445:1454	arg1	strength					1456:1463	defective stem mechanical strength	1430:1463	defective stem mechanical strength	1430:1463	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	9	41	theme	groups	1810:1815	arg1	involvement					1767:1777	the involvement	1763:1777	the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis	1763:1915	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	1	42	attach	present	113:119	arg2	hemicellulose					99:111	the major hemicellulose	89:111	the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues	89:187	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	1	42	attach	present	113:119	arg2	Xylan					80:84	Xylan	80:84	Xylan	80:84	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	1	42	attach	present	113:119	arg1	walls					156:160	both primary and secondary cell walls	124:160	both primary and secondary cell walls of rice vegetative tissues	124:187	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	7	43	theme	defective	1430:1438	arg1	strength					1456:1463	defective stem mechanical strength	1430:1463	defective stem mechanical strength	1430:1463	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	3	44	theme	nonredundant	539:550	arg1	groups					552:557	2 functionally nonredundant groups	524:557	2 functionally nonredundant groups	524:557	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	3	44	theme	nonredundant	539:550	arg1	homolog					570:576	IRX9/IRX9 homolog	560:576	IRX9/IRX9 homolog	560:576	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	3	44	theme	nonredundant	539:550	arg1	homolog					594:600	IRX14/IRX14 homolog	582:600	IRX14/IRX14 homolog	582:600	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	5	45	theme	stem	885:888	arg1	microsomes					890:899	rice stem microsomes	880:899	rice stem microsomes	880:899	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	7	46	theme	irx9	1405:1408	arg1	mutation					1410:1417	the irx9 mutation	1401:1417	the irx9 mutation	1401:1417	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	9	47	theme	IRX9	1839:1842	arg1	homologs					1844:1851	IRX9 homologs	1839:1851	IRX9 homologs	1839:1851	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	9	47	theme	IRX9	1839:1842	arg1	OsGT43A					1818:1824	OsGT43A	1818:1824	OsGT43A	1818:1824	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	3	48	theme	IRX14/IRX14	582:592	arg1	groups					552:557	2 functionally nonredundant groups	524:557	2 functionally nonredundant groups	524:557	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	3	48	theme	IRX14/IRX14	582:592	arg1	homolog					594:600	IRX14/IRX14 homolog	582:600	IRX14/IRX14 homolog	582:600	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	1	49	theme	vegetative	170:179	arg1	tissues					181:187	rice vegetative tissues	165:187	rice vegetative tissues	165:187	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	7	50	theme	mutant	1370:1375	arg1	phenotypes					1377:1386	the mutant phenotypes	1366:1386	the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity	1366:1568	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	8	51	theme	xylan	1659:1663	arg1	phenotype					1665:1673	the defective xylan phenotype	1645:1673	the defective xylan phenotype in the irx14 mutant	1645:1693	On the other hand, OsGT43J but not OsGT43A, OsGT43E, and OsGT43H restored the defective xylan phenotype in the irx14 mutant.
24525904	6	52	theme	OsGT43	1102:1107	arg1	proteins					1109:1116	OsGT43 proteins	1102:1116	OsGT43 proteins	1102:1116	OsGT43 proteins were shown to be localized in the Golgi, where xylan biosynthesis occurs.
24525904	2	53	theme	factors	216:222	arg1	one					205:207	one	205:207	one	205:207	Since xylan is one of the factors contributing to biomass recalcitrance, understanding how xylan is synthesized in rice will potentially provide tools to modify grass biomass composition better suited for biofuel production.
24525904	2	53	theme	factors	216:222	arg1	factors					216:222	the factors	212:222	the factors contributing to biomass recalcitrance	212:260	Since xylan is one of the factors contributing to biomass recalcitrance, understanding how xylan is synthesized in rice will potentially provide tools to modify grass biomass composition better suited for biofuel production.
24525904	5	54	theme	xylosyltransferase	849:866	arg1	activity					868:875	xylan xylosyltransferase activity	843:875	xylan xylosyltransferase activity in rice stem microsomes	843:899	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	8	55	theme	irx14	1682:1686	arg1	mutant					1688:1693	the irx14 mutant	1678:1693	the irx14 mutant	1678:1693	On the other hand, OsGT43J but not OsGT43A, OsGT43E, and OsGT43H restored the defective xylan phenotype in the irx14 mutant.
24525904	1	56	theme	present	113:119	arg1	Xylan					80:84	Xylan	80:84	Xylan	80:84	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	1	56	theme	present	113:119	arg1	hemicellulose					99:111	the major hemicellulose	89:111	the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues	89:187	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	7	57	theme	xylosyltransferase	1542:1559	arg1	activity					1561:1568	xylosyltransferase activity	1542:1568	xylosyltransferase activity	1542:1568	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	6	58	theme	xylan	1165:1169	arg1	biosynthesis					1171:1182	xylan biosynthesis	1165:1182	xylan biosynthesis	1165:1182	OsGT43 proteins were shown to be localized in the Golgi, where xylan biosynthesis occurs.
24525904	5	59	from	roles	922:926	arg1	biosynthesis					1088:1099	xylan biosynthesis	1082:1099	xylan biosynthesis	1082:1099	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	60	theme	representative	933:946	arg1	OsGT43A					967:973	OsGT43A	967:973	OsGT43A	967:973	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	60	theme	representative	933:946	arg1	OsGT43H					1021:1027	OsGT43H	1021:1027	OsGT43H	1021:1027	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	60	theme	representative	933:946	arg1	OsGT43J					1052:1058	OsGT43J	1052:1058	OsGT43J	1052:1058	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	60	theme	representative	933:946	arg1	members					958:964	4 representative rice GT43 members	931:964	4 representative rice GT43 members	931:964	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	60	theme	representative	933:946	arg1	OsGT43E					994:1000	OsGT43E	994:1000	OsGT43E	994:1000	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	3	61	theme	xylan	620:624	arg1	elongation					635:644	xylan backbone elongation	620:644	xylan backbone elongation	620:644	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	5	62	from	microsomes	890:899	arg1	analysis					831:838	biochemical analysis	819:838	biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes	819:899	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	7	63	theme	chain	1524:1528	arg1	length					1530:1535	xylan chain length	1518:1535	xylan chain length	1518:1535	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	9	64	theme	xylan	1889:1893	arg1	biosynthesis					1904:1915	xylan backbone biosynthesis	1889:1915	xylan backbone biosynthesis	1889:1915	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	4	65	theme	GT43	696:699	arg1	members					701:707	family GT43 members	689:707	family GT43 members	689:707	The rice genome harbors 10 genes encoding family GT43 members and it is currently unknown whether they are all involved in xylan biosynthesis.
24525904	5	66	theme	xylan	1082:1086	arg1	biosynthesis					1088:1099	xylan biosynthesis	1082:1099	xylan biosynthesis	1082:1099	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	1	67	theme	cell	151:154	arg1	walls					156:160	both primary and secondary cell walls	124:160	both primary and secondary cell walls of rice vegetative tissues	124:187	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	0	68	from	roles	11:15	arg1	biosynthesis					66:77	xylan biosynthesis	60:77	xylan biosynthesis	60:77	Functional roles of rice glycosyltransferase family GT43 in xylan biosynthesis.
24525904	3	69	from	Studies	415:421	arg1	Arabidopsis					448:458	Arabidopsis	448:458	Arabidopsis	448:458	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	1	70	from	walls	156:160	arg1	present					113:119	present	113:119	present	113:119	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	7	71	theme	side	1505:1508	arg1	chains					1510:1515	GlcA side chains	1500:1515	GlcA side chains	1500:1515	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	5	72	from	analysis	831:838	arg1	microsomes					890:899	rice stem microsomes	880:899	rice stem microsomes	880:899	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	73	theme	GT43	953:956	arg1	OsGT43A					967:973	OsGT43A	967:973	OsGT43A	967:973	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	73	theme	GT43	953:956	arg1	OsGT43H					1021:1027	OsGT43H	1021:1027	OsGT43H	1021:1027	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	73	theme	GT43	953:956	arg1	OsGT43J					1052:1058	OsGT43J	1052:1058	OsGT43J	1052:1058	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	73	theme	GT43	953:956	arg1	members					958:964	4 representative rice GT43 members	931:964	4 representative rice GT43 members	931:964	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	5	73	theme	GT43	953:956	arg1	OsGT43E					994:1000	OsGT43E	994:1000	OsGT43E	994:1000	In this report, we performed biochemical analysis of xylan xylosyltransferase activity in rice stem microsomes and investigated the roles of 4 representative rice GT43 members, OsGT43A (LOC_Os05 g03174), OsGT43E (LOC_Os05 g48600), OsGT43H (LOC_Os04 g01280), and OsGT43J (LOC_Os06 g47340), in xylan biosynthesis.
24525904	9	74	theme	GT43	1733:1736	arg1	family					1738:1743	the rice GT43 family	1724:1743	the rice GT43 family	1724:1743	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	3	75	theme	xylan	426:430	arg1	biosynthesis					432:443	xylan biosynthesis	426:443	xylan biosynthesis in Arabidopsis	426:458	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	0	76	theme	rice	20:23	arg1	family					45:50	rice glycosyltransferase family GT43	20:55	rice glycosyltransferase family GT43	20:55	Functional roles of rice glycosyltransferase family GT43 in xylan biosynthesis.
24525904	1	77	theme	major	93:97	arg1	Xylan					80:84	Xylan	80:84	Xylan	80:84	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	1	77	theme	major	93:97	arg1	hemicellulose					99:111	the major hemicellulose	89:111	the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues	89:187	Xylan is the major hemicellulose present in both primary and secondary cell walls of rice vegetative tissues.
24525904	7	78	theme	irx14	1266:1270	arg1	mutants					1272:1278	irx14 mutants	1266:1278	irx14 mutants	1266:1278	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	0	79	theme	family	45:50	arg1	roles					11:15	Functional roles	0:15	Functional roles of rice glycosyltransferase family GT43 in xylan biosynthesis	0:77	Functional roles of rice glycosyltransferase family GT43 in xylan biosynthesis.
24525904	7	80	theme	GlcA	1500:1503	arg1	chains					1510:1515	GlcA side chains	1500:1515	GlcA side chains	1500:1515	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	3	81	from	Arabidopsis	448:458	arg1	Studies					415:421	Studies	415:421	Studies of xylan biosynthesis in Arabidopsis	415:458	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	2	82	theme	biofuel	395:401	arg1	production					403:412	biofuel production	395:412	biofuel production	395:412	Since xylan is one of the factors contributing to biomass recalcitrance, understanding how xylan is synthesized in rice will potentially provide tools to modify grass biomass composition better suited for biofuel production.
24525904	3	83	theme	GT43	486:489	arg1	glycosyltransferases					491:510	family GT43 glycosyltransferases	479:510	family GT43 glycosyltransferases	479:510	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	7	84	theme	vessel	1466:1471	arg1	morphology					1473:1482	vessel morphology	1466:1482	vessel morphology	1466:1482	Complementation analysis by expression of OsGT43s in Arabidopsis irx9 and irx14 mutants demonstrated that OsGT43A and OsGT43E but not OsGT43H and OsGT43J were able to rescue the mutant phenotypes conferred by the irx9 mutation, including defective stem mechanical strength, vessel morphology, xylan content, GlcA side chains, xylan chain length, and xylosyltransferase activity.
24525904	9	85	theme	nonredundant	1797:1808	arg1	OsGT43J					1858:1864	OsGT43J	1858:1864	OsGT43J	1858:1864	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	9	85	theme	nonredundant	1797:1808	arg1	OsGT43E					1830:1836	OsGT43E	1830:1836	OsGT43E	1830:1836	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	9	85	theme	nonredundant	1797:1808	arg1	OsGT43A					1818:1824	OsGT43A	1818:1824	OsGT43A	1818:1824	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	9	85	theme	nonredundant	1797:1808	arg1	groups					1810:1815	2 functionally nonredundant groups	1782:1815	2 functionally nonredundant groups	1782:1815	These results indicate that the rice GT43 family evolved to retain the involvement of 2 functionally nonredundant groups, OsGT43A and OsGT43E (IRX9 homologs) vs. OsGT43J (an IRX14 homolog), in xylan backbone biosynthesis.
24525904	3	86	from	biosynthesis	432:443	arg1	Arabidopsis					448:458	Arabidopsis	448:458	Arabidopsis	448:458	Studies of xylan biosynthesis in Arabidopsis have revealed that family GT43 glycosyltransferases, which form 2 functionally nonredundant groups, IRX9/IRX9 homolog and IRX14/IRX14 homolog, are required for xylan backbone elongation.
24525904	2	87	theme	grass	351:355	arg1	composition					365:375	grass biomass composition	351:375	grass biomass composition better suited for biofuel production	351:412	Since xylan is one of the factors contributing to biomass recalcitrance, understanding how xylan is synthesized in rice will potentially provide tools to modify grass biomass composition better suited for biofuel production.
22784213	6	0	dep	rapidity	942:949	arg1	the					938:940	the	938:940	the	938:940	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	2	1	theme	various	332:338	arg1	instruments					351:361	various analytical instruments	332:361	various analytical instruments	332:361	To fulfil this requirement, various analytical instruments have been subjected to scientific and clinical evaluation.
22784213	1	2	theme	preterm	250:256	arg1	fed					258:260	preterm fed	250:260	preterm fed	250:260	The variability of human milk (HM) composition renders analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk.
22784213	8	3	theme	amount	1234:1239	arg1	underestimation					1211:1225	underestimation	1211:1225	underestimation	1211:1225	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	8	3	theme	amount	1234:1239	arg1	content					1199:1205	lipid content	1193:1205	lipid content	1193:1205	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	6	4	theme	HMA	848:850	arg1	use					829:831	The use	825:831	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM	825:905	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	1	5	theme	HM	164:165	arg1	composition					168:178	human milk (HM) composition	152:178	human milk (HM) composition	152:178	The variability of human milk (HM) composition renders analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk.
22784213	1	6	theme	fed	258:260	arg1	nutrition					237:245	optimal nutrition	229:245	optimal nutrition of preterm fed	229:260	The variability of human milk (HM) composition renders analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk.
22784213	3	7	theme	study	444:448	arg1	objective					426:434	The objective	422:434	The objective of this study	422:448	The objective of this study was to evaluate the suitability of a rapid method for the analysis of macronutrients in HM as compared with the analytical methods applied by cow's milk industry.
22784213	6	8	theme	infrared	839:846	arg1	HMA					848:850	an infrared HMA	836:850	an infrared HMA for the analysis of lipids, proteins and lactose in HM	836:905	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	3	9	theme	method	493:498	arg1	suitability					470:480	the suitability	466:480	the suitability of a rapid method for the analysis of macronutrients in HM	466:539	The objective of this study was to evaluate the suitability of a rapid method for the analysis of macronutrients in HM as compared with the analytical methods applied by cow's milk industry.
22784213	8	10	theme	contents	1265:1272	arg1	proteins					1244:1251	proteins	1244:1251	proteins	1244:1251	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	8	10	theme	contents	1265:1272	arg1	contents					1265:1272	lactose contents	1257:1272	lactose contents	1257:1272	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	8	10	theme	contents	1265:1272	arg1	amount					1234:1239	the amount	1230:1239	the amount of proteins and lactose contents	1230:1272	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	0	11	theme	macronutrients	103:116	arg1	determination					86:98	the determination	82:98	the determination of macronutrients in human milk	82:130	Comparison of mid-infrared transmission spectroscopy with biochemical methods for the determination of macronutrients in human milk.
22784213	8	12	theme	lactose	1257:1263	arg1	contents					1265:1272	lactose contents	1257:1272	lactose contents	1257:1272	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	3	13	theme	macronutrients	520:533	arg1	analysis					508:515	the analysis	504:515	the analysis of macronutrients	504:533	The objective of this study was to evaluate the suitability of a rapid method for the analysis of macronutrients in HM as compared with the analytical methods applied by cow's milk industry.
22784213	4	14	from	donors	633:638	arg1	milk					620:623	Mature milk	613:623	Mature milk from 39 donors	613:638	Mature milk from 39 donors was analysed using an infrared human milk analyser (HMA) and compared with biochemical reference laboratory methods.
22784213	4	15	theme	milk	677:680	arg1	HMA					692:694	HMA	692:694	HMA	692:694	Mature milk from 39 donors was analysed using an infrared human milk analyser (HMA) and compared with biochemical reference laboratory methods.
22784213	4	15	theme	milk	677:680	arg1	analyser					682:689	an infrared human milk analyser	659:689	an infrared human milk analyser (HMA)	659:695	Mature milk from 39 donors was analysed using an infrared human milk analyser (HMA) and compared with biochemical reference laboratory methods.
22784213	6	16	dep	regards	930:936	arg1	volume					987:992	the required sample volume	967:992	the required sample volume	967:992	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	6	16	dep	regards	930:936	arg1	simplicity					952:961	simplicity	952:961	simplicity	952:961	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	6	16	dep	regards	930:936	arg1	rapidity					942:949	rapidity	942:949	rapidity	942:949	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	6	16	dep	regards	930:936	arg1	regards					930:936	regards the rapidity, simplicity and the required sample volume	930:992	regards the rapidity, simplicity and the required sample volume	930:992	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	7	17	theme	good	1019:1022	arg1	linearity					1024:1032	good linearity	1019:1032	good linearity	1019:1032	The instrument afforded good linearity and precision in application to all three nutrients.
22784213	4	18	theme	reference	727:735	arg1	methods					748:754	biochemical reference laboratory methods	715:754	biochemical reference laboratory methods	715:754	Mature milk from 39 donors was analysed using an infrared human milk analyser (HMA) and compared with biochemical reference laboratory methods.
22784213	5	19	theme	tests	818:822	arg1	use					799:801	the use	795:801	the use of paired data tests	795:822	The statistical analysis was based on the use of paired data tests.
22784213	5	20	theme	paired	806:811	arg1	tests					818:822	paired data tests	806:822	paired data tests	806:822	The statistical analysis was based on the use of paired data tests.
22784213	0	21	theme	human	121:125	arg1	milk					127:130	human milk	121:130	human milk	121:130	Comparison of mid-infrared transmission spectroscopy with biochemical methods for the determination of macronutrients in human milk.
22784213	6	22	from	HM	904:905	arg1	analysis					860:867	the analysis	856:867	the analysis of lipids, proteins and lactose in HM	856:905	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	8	23	theme	underestimation	1211:1225	arg1	overestimation					1171:1184	overestimation	1171:1184	overestimation of the lipid content and underestimation of the amount of proteins and lactose contents	1171:1272	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	4	24	theme	biochemical	715:725	arg1	methods					748:754	biochemical reference laboratory methods	715:754	biochemical reference laboratory methods	715:754	Mature milk from 39 donors was analysed using an infrared human milk analyser (HMA) and compared with biochemical reference laboratory methods.
22784213	1	25	theme	composition	168:178	arg1	variability					137:147	The variability	133:147	The variability of human milk (HM) composition	133:178	The variability of human milk (HM) composition renders analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk.
22784213	5	26	theme	statistical	761:771	arg1	analysis					773:780	The statistical analysis	757:780	The statistical analysis	757:780	The statistical analysis was based on the use of paired data tests.
22784213	6	27	theme	proteins	880:887	arg1	analysis					860:867	the analysis	856:867	the analysis of lipids, proteins and lactose in HM	856:905	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	4	28	theme	Mature	613:618	arg1	milk					620:623	Mature milk	613:623	Mature milk from 39 donors	613:638	Mature milk from 39 donors was analysed using an infrared human milk analyser (HMA) and compared with biochemical reference laboratory methods.
22784213	3	29	from	suitability	470:480	arg1	HM					538:539	HM	538:539	HM	538:539	The objective of this study was to evaluate the suitability of a rapid method for the analysis of macronutrients in HM as compared with the analytical methods applied by cow's milk industry.
22784213	6	30	theme	sample	980:985	arg1	volume					987:992	the required sample volume	967:992	the required sample volume	967:992	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	6	30	theme	sample	980:985	arg1	regards					930:936	regards the rapidity, simplicity and the required sample volume	930:992	regards the rapidity, simplicity and the required sample volume	930:992	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	0	31	theme	transmission	27:38	arg1	spectroscopy					40:51	mid-infrared transmission spectroscopy	14:51	mid-infrared transmission spectroscopy	14:51	Comparison of mid-infrared transmission spectroscopy with biochemical methods for the determination of macronutrients in human milk.
22784213	6	32	theme	lactose	893:899	arg1	analysis					860:867	the analysis	856:867	the analysis of lipids, proteins and lactose in HM	856:905	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	9	33	theme	HM	1351:1352	arg1	analysis					1339:1346	rapid analysis	1333:1346	rapid analysis of HM	1333:1352	The use of mid-infrared HMA might become the standard for rapid analysis of HM once standardisation and rigorous and systematic calibration is provided.
22784213	8	34	theme	proteins	1244:1251	arg1	proteins					1244:1251	proteins	1244:1251	proteins	1244:1251	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	8	34	theme	proteins	1244:1251	arg1	contents					1265:1272	lactose contents	1257:1272	lactose contents	1257:1272	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	8	34	theme	proteins	1244:1251	arg1	amount					1234:1239	the amount	1230:1239	the amount of proteins and lactose contents	1230:1272	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	6	35	theme	required	971:978	arg1	volume					987:992	the required sample volume	967:992	the required sample volume	967:992	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	6	35	theme	required	971:978	arg1	regards					930:936	regards the rapidity, simplicity and the required sample volume	930:992	regards the rapidity, simplicity and the required sample volume	930:992	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	0	36	theme	mid-infrared	14:25	arg1	spectroscopy					40:51	mid-infrared transmission spectroscopy	14:51	mid-infrared transmission spectroscopy	14:51	Comparison of mid-infrared transmission spectroscopy with biochemical methods for the determination of macronutrients in human milk.
22784213	6	37	from	proteins	880:887	arg1	HM					904:905	HM	904:905	HM	904:905	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	1	38	dep	nutrition	237:245	arg1	either					262:267	either	262:267	either	262:267	The variability of human milk (HM) composition renders analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk.
22784213	3	39	theme	rapid	487:491	arg1	method					493:498	a rapid method	485:498	a rapid method for the analysis of macronutrients	485:533	The objective of this study was to evaluate the suitability of a rapid method for the analysis of macronutrients in HM as compared with the analytical methods applied by cow's milk industry.
22784213	2	40	theme	clinical	401:408	arg1	evaluation					410:419	scientific and clinical evaluation	386:419	scientific and clinical evaluation	386:419	To fulfil this requirement, various analytical instruments have been subjected to scientific and clinical evaluation.
22784213	1	41	theme	own	285:287	arg1	mother					289:294	own mother's	285:296	own mother's	285:296	The variability of human milk (HM) composition renders analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk.
22784213	0	42	from	determination	86:98	arg1	milk					127:130	human milk	121:130	human milk	121:130	Comparison of mid-infrared transmission spectroscopy with biochemical methods for the determination of macronutrients in human milk.
22784213	3	43	theme	analytical	562:571	arg1	methods					573:579	the analytical methods	558:579	the analytical methods applied by cow's milk industry	558:610	The objective of this study was to evaluate the suitability of a rapid method for the analysis of macronutrients in HM as compared with the analytical methods applied by cow's milk industry.
22784213	1	44	theme	components	204:213	arg1	analysis					188:195	analysis	188:195	analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk	188:301	The variability of human milk (HM) composition renders analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk.
22784213	0	45	theme	spectroscopy	40:51	arg1	Comparison					0:9	Comparison	0:9	Comparison of mid-infrared transmission spectroscopy with biochemical methods for the determination of macronutrients in human milk.	0:131	Comparison of mid-infrared transmission spectroscopy with biochemical methods for the determination of macronutrients in human milk.
22784213	6	46	from	lactose	893:899	arg1	HM					904:905	HM	904:905	HM	904:905	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	3	47	theme	milk	598:601	arg1	industry					603:610	cow's milk industry	592:610	cow's milk industry	592:610	The objective of this study was to evaluate the suitability of a rapid method for the analysis of macronutrients in HM as compared with the analytical methods applied by cow's milk industry.
22784213	1	48	theme	essential	215:223	arg1	components					204:213	its components	200:213	its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk	200:301	The variability of human milk (HM) composition renders analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk.
22784213	8	49	theme	reference	1147:1155	arg1	methods					1157:1163	the reference methods	1143:1163	the reference methods	1143:1163	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	6	50	theme	lipids	872:877	arg1	analysis					860:867	the analysis	856:867	the analysis of lipids, proteins and lactose in HM	856:905	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	9	51	theme	systematic	1392:1401	arg1	calibration					1403:1413	standardisation and rigorous and systematic calibration	1359:1413	standardisation and rigorous and systematic calibration	1359:1413	The use of mid-infrared HMA might become the standard for rapid analysis of HM once standardisation and rigorous and systematic calibration is provided.
22784213	4	52	theme	human	671:675	arg1	HMA					692:694	HMA	692:694	HMA	692:694	Mature milk from 39 donors was analysed using an infrared human milk analyser (HMA) and compared with biochemical reference laboratory methods.
22784213	4	52	theme	human	671:675	arg1	analyser					682:689	an infrared human milk analyser	659:689	an infrared human milk analyser (HMA)	659:695	Mature milk from 39 donors was analysed using an infrared human milk analyser (HMA) and compared with biochemical reference laboratory methods.
22784213	0	53	theme	biochemical	58:68	arg1	methods					70:76	biochemical methods	58:76	biochemical methods for the determination of macronutrients in human milk	58:130	Comparison of mid-infrared transmission spectroscopy with biochemical methods for the determination of macronutrients in human milk.
22784213	9	54	theme	mid-infrared	1286:1297	arg1	HMA					1299:1301	mid-infrared HMA	1286:1301	mid-infrared HMA	1286:1301	The use of mid-infrared HMA might become the standard for rapid analysis of HM once standardisation and rigorous and systematic calibration is provided.
22784213	6	55	from	analysis	860:867	arg1	HM					904:905	HM	904:905	HM	904:905	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	4	56	theme	infrared	662:669	arg1	HMA					692:694	HMA	692:694	HMA	692:694	Mature milk from 39 donors was analysed using an infrared human milk analyser (HMA) and compared with biochemical reference laboratory methods.
22784213	4	56	theme	infrared	662:669	arg1	analyser					682:689	an infrared human milk analyser	659:689	an infrared human milk analyser (HMA)	659:695	Mature milk from 39 donors was analysed using an infrared human milk analyser (HMA) and compared with biochemical reference laboratory methods.
22784213	9	57	theme	HMA	1299:1301	arg1	use					1279:1281	The use	1275:1281	The use of mid-infrared HMA	1275:1301	The use of mid-infrared HMA might become the standard for rapid analysis of HM once standardisation and rigorous and systematic calibration is provided.
22784213	8	58	theme	content	1199:1205	arg1	overestimation					1171:1184	overestimation	1171:1184	overestimation of the lipid content and underestimation of the amount of proteins and lactose contents	1171:1272	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	0	59	with	Comparison	0:9	arg1	methods					70:76	biochemical methods	58:76	biochemical methods for the determination of macronutrients in human milk	58:130	Comparison of mid-infrared transmission spectroscopy with biochemical methods for the determination of macronutrients in human milk.
22784213	8	60	theme	lipid	1193:1197	arg1	content					1199:1205	lipid content	1193:1205	lipid content	1193:1205	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	6	61	from	lipids	872:877	arg1	HM					904:905	HM	904:905	HM	904:905	The use of an infrared HMA for the analysis of lipids, proteins and lactose in HM proved satisfactory as regards the rapidity, simplicity and the required sample volume.
22784213	9	62	theme	rapid	1333:1337	arg1	analysis					1339:1346	rapid analysis	1333:1346	rapid analysis of HM	1333:1352	The use of mid-infrared HMA might become the standard for rapid analysis of HM once standardisation and rigorous and systematic calibration is provided.
22784213	4	63	theme	laboratory	737:746	arg1	methods					748:754	biochemical reference laboratory methods	715:754	biochemical reference laboratory methods	715:754	Mature milk from 39 donors was analysed using an infrared human milk analyser (HMA) and compared with biochemical reference laboratory methods.
22784213	8	64	dep	content	1199:1205	arg1	the					1189:1191	the	1189:1191	the	1189:1191	However, accuracy was not acceptable when compared with the reference methods, with overestimation of the lipid content and underestimation of the amount of proteins and lactose contents.
22784213	2	65	theme	analytical	340:349	arg1	instruments					351:361	various analytical instruments	332:361	various analytical instruments	332:361	To fulfil this requirement, various analytical instruments have been subjected to scientific and clinical evaluation.
22784213	1	66	theme	optimal	229:235	arg1	nutrition					237:245	optimal nutrition	229:245	optimal nutrition of preterm fed	229:260	The variability of human milk (HM) composition renders analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk.
22784213	2	67	theme	scientific	386:395	arg1	evaluation					410:419	scientific and clinical evaluation	386:419	scientific and clinical evaluation	386:419	To fulfil this requirement, various analytical instruments have been subjected to scientific and clinical evaluation.
22784213	9	68	theme	rigorous	1379:1386	arg1	calibration					1403:1413	standardisation and rigorous and systematic calibration	1359:1413	standardisation and rigorous and systematic calibration	1359:1413	The use of mid-infrared HMA might become the standard for rapid analysis of HM once standardisation and rigorous and systematic calibration is provided.
22784213	1	69	theme	human	152:156	arg1	composition					168:178	human milk (HM) composition	152:178	human milk (HM) composition	152:178	The variability of human milk (HM) composition renders analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk.
22784213	5	70	theme	data	813:816	arg1	tests					818:822	paired data tests	806:822	paired data tests	806:822	The statistical analysis was based on the use of paired data tests.
22784213	9	71	theme	standardisation	1359:1373	arg1	calibration					1403:1413	standardisation and rigorous and systematic calibration	1359:1413	standardisation and rigorous and systematic calibration	1359:1413	The use of mid-infrared HMA might become the standard for rapid analysis of HM once standardisation and rigorous and systematic calibration is provided.
22784213	1	72	theme	milk	158:161	arg1	composition					168:178	human milk (HM) composition	152:178	human milk (HM) composition	152:178	The variability of human milk (HM) composition renders analysis of its components essential for optimal nutrition of preterm fed either with donor's or own mother's milk.
29253988	4	0	from	effects	574:580	arg1	behaviours					658:667	morphological, swelling and creep/recovery behaviours	615:667	morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels	615:691	The effects of the concentration of WS/KL on morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels were investigated.
29253988	7	1	theme	non-toxic	1064:1072	arg1	hydrogels					1049:1057	the final hyaluronan/lignin hydrogels	1021:1057	the final hyaluronan/lignin hydrogels	1021:1057	The cytotoxicity tests proved that glycinated KL lignin limits the viability of cells only slightly, and the final hyaluronan/lignin hydrogels were non-toxic materials.
29253988	7	1	theme	non-toxic	1064:1072	arg1	materials					1074:1082	non-toxic materials	1064:1082	non-toxic materials	1064:1082	The cytotoxicity tests proved that glycinated KL lignin limits the viability of cells only slightly, and the final hyaluronan/lignin hydrogels were non-toxic materials.
29253988	7	2	theme	cytotoxicity	920:931	arg1	tests					933:937	The cytotoxicity tests	916:937	The cytotoxicity tests	916:937	The cytotoxicity tests proved that glycinated KL lignin limits the viability of cells only slightly, and the final hyaluronan/lignin hydrogels were non-toxic materials.
29253988	4	3	theme	hyaluronan	672:681	arg1	hydrogels					683:691	hyaluronan hydrogels	672:691	hyaluronan hydrogels	672:691	The effects of the concentration of WS/KL on morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels were investigated.
29253988	1	4	theme	soluble	159:165	arg1	lignin					184:189	glycinated Kraft lignin	167:189	water soluble glycinated Kraft lignin (WS/KL)	153:197	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	1	4	theme	soluble	159:165	arg1	WS/KL					192:196	WS/KL	192:196	WS/KL	192:196	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	1	5	theme	hyaluronan	251:260	arg1	hydrogels					269:277	hyaluronan (NaHy) hydrogels	251:277	hyaluronan (NaHy) hydrogels	251:277	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	0	6	theme	viscoelastic	80:91	arg1	properties					93:102	viscoelastic properties	80:102	viscoelastic properties	80:102	Hyaluronan hydrogels modified by glycinated Kraft lignin: Morphology, swelling, viscoelastic properties and biocompatibility.
29253988	5	7	dep	3	834:834	arg1	to					831:832	to	831:832	to	831:832	It was detected that the creep resistance and creep recovery of NaHy hydrogels was improved by the incorporation of up to 3% (w/w) of WS/KL.
29253988	7	8	theme	final	1025:1029	arg1	hydrogels					1049:1057	the final hyaluronan/lignin hydrogels	1021:1057	the final hyaluronan/lignin hydrogels	1021:1057	The cytotoxicity tests proved that glycinated KL lignin limits the viability of cells only slightly, and the final hyaluronan/lignin hydrogels were non-toxic materials.
29253988	7	8	theme	final	1025:1029	arg1	materials					1074:1082	non-toxic materials	1064:1082	non-toxic materials	1064:1082	The cytotoxicity tests proved that glycinated KL lignin limits the viability of cells only slightly, and the final hyaluronan/lignin hydrogels were non-toxic materials.
29253988	4	9	theme	concentration	589:601	arg1	effects					574:580	The effects	570:580	The effects of the concentration of WS/KL on morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels	570:691	The effects of the concentration of WS/KL on morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels were investigated.
29253988	1	10	theme	lignin	184:189	arg1	addition					141:148	the addition	137:148	the addition of water soluble glycinated Kraft lignin (WS/KL)	137:197	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	1	11	theme	glycinated	167:176	arg1	lignin					184:189	glycinated Kraft lignin	167:189	water soluble glycinated Kraft lignin (WS/KL)	153:197	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	1	11	theme	glycinated	167:176	arg1	WS/KL					192:196	WS/KL	192:196	WS/KL	192:196	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	5	12	theme	WS/KL	846:850	arg1	w/w					838:840	w/w	838:840	w/w	838:840	It was detected that the creep resistance and creep recovery of NaHy hydrogels was improved by the incorporation of up to 3% (w/w) of WS/KL.
29253988	5	12	theme	WS/KL	846:850	arg1	WS/KL					846:850	WS/KL	846:850	WS/KL	846:850	It was detected that the creep resistance and creep recovery of NaHy hydrogels was improved by the incorporation of up to 3% (w/w) of WS/KL.
29253988	5	12	theme	WS/KL	846:850	arg1	%					835:835	up to 3%	828:835	up to 3% (w/w) of WS/KL	828:850	It was detected that the creep resistance and creep recovery of NaHy hydrogels was improved by the incorporation of up to 3% (w/w) of WS/KL.
29253988	5	13	theme	creep	758:762	arg1	recovery					764:771	creep recovery	758:771	creep recovery	758:771	It was detected that the creep resistance and creep recovery of NaHy hydrogels was improved by the incorporation of up to 3% (w/w) of WS/KL.
29253988	1	14	theme	Kraft	178:182	arg1	lignin					184:189	glycinated Kraft lignin	167:189	water soluble glycinated Kraft lignin (WS/KL)	153:197	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	1	14	theme	Kraft	178:182	arg1	WS/KL					192:196	WS/KL	192:196	WS/KL	192:196	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	5	15	theme	hydrogels	781:789	arg1	resistance					743:752	creep resistance	737:752	creep resistance	737:752	It was detected that the creep resistance and creep recovery of NaHy hydrogels was improved by the incorporation of up to 3% (w/w) of WS/KL.
29253988	5	15	theme	hydrogels	781:789	arg1	recovery					764:771	creep recovery	758:771	creep recovery	758:771	It was detected that the creep resistance and creep recovery of NaHy hydrogels was improved by the incorporation of up to 3% (w/w) of WS/KL.
29253988	4	16	theme	morphological	615:627	arg1	behaviours					658:667	morphological, swelling and creep/recovery behaviours	615:667	morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels	615:691	The effects of the concentration of WS/KL on morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels were investigated.
29253988	2	17	theme	Kraft	365:369	arg1	lignin					371:376	Kraft lignin	365:376	Kraft lignin	365:376	Water soluble lignin was obtained by the modification of Kraft lignin via a Mannich reaction.
29253988	3	18	theme	new	536:538	arg1	hydrogels					559:567	new advanced composite hydrogels	536:567	new advanced composite hydrogels	536:567	It was found that WS/KL is highly compatible with hyaluronan due to its improved water solubility, which favours its use in designing new advanced composite hydrogels.
29253988	4	19	theme	swelling	630:637	arg1	behaviours					658:667	morphological, swelling and creep/recovery behaviours	615:667	morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels	615:691	The effects of the concentration of WS/KL on morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels were investigated.
29253988	1	20	theme	hydrogels	269:277	arg1	biocompatibility					231:246	biocompatibility	231:246	biocompatibility	231:246	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	1	20	theme	hydrogels	269:277	arg1	stability					217:225	mechanical stability	206:225	mechanical stability	206:225	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	5	21	dep	resistance	743:752	arg1	the					733:735	the	733:735	the	733:735	It was detected that the creep resistance and creep recovery of NaHy hydrogels was improved by the incorporation of up to 3% (w/w) of WS/KL.
29253988	1	22	from	Effects	126:132	arg1	biocompatibility					231:246	biocompatibility	231:246	biocompatibility	231:246	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	1	22	from	Effects	126:132	arg1	stability					217:225	mechanical stability	206:225	mechanical stability	206:225	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	7	23	theme	hyaluronan/lignin	1031:1047	arg1	hydrogels					1049:1057	the final hyaluronan/lignin hydrogels	1021:1057	the final hyaluronan/lignin hydrogels	1021:1057	The cytotoxicity tests proved that glycinated KL lignin limits the viability of cells only slightly, and the final hyaluronan/lignin hydrogels were non-toxic materials.
29253988	7	23	theme	hyaluronan/lignin	1031:1047	arg1	materials					1074:1082	non-toxic materials	1064:1082	non-toxic materials	1064:1082	The cytotoxicity tests proved that glycinated KL lignin limits the viability of cells only slightly, and the final hyaluronan/lignin hydrogels were non-toxic materials.
29253988	3	24	theme	improved	474:481	arg1	solubility					489:498	its improved water solubility	470:498	its improved water solubility	470:498	It was found that WS/KL is highly compatible with hyaluronan due to its improved water solubility, which favours its use in designing new advanced composite hydrogels.
29253988	1	25	theme	addition	141:148	arg1	Effects					126:132	Effects	126:132	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels	126:277	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	0	26	theme	Kraft	44:48	arg1	lignin					50:55	glycinated Kraft lignin	33:55	glycinated Kraft lignin	33:55	Hyaluronan hydrogels modified by glycinated Kraft lignin: Morphology, swelling, viscoelastic properties and biocompatibility.
29253988	6	27	theme	hydrogels	891:899	arg1	capacity					879:886	the swelling capacity	866:886	the swelling capacity of hydrogels	866:899	In contrast, the swelling capacity of hydrogels was decreased.
29253988	7	28	theme	cells	996:1000	arg1	viability					983:991	the viability	979:991	the viability of cells	979:1000	The cytotoxicity tests proved that glycinated KL lignin limits the viability of cells only slightly, and the final hyaluronan/lignin hydrogels were non-toxic materials.
29253988	2	29	theme	Mannich	384:390	arg1	reaction					392:399	a Mannich reaction	382:399	a Mannich reaction	382:399	Water soluble lignin was obtained by the modification of Kraft lignin via a Mannich reaction.
29253988	0	30	theme	glycinated	33:42	arg1	lignin					50:55	glycinated Kraft lignin	33:55	glycinated Kraft lignin	33:55	Hyaluronan hydrogels modified by glycinated Kraft lignin: Morphology, swelling, viscoelastic properties and biocompatibility.
29253988	3	31	with	compatible	436:445	arg1	hyaluronan					452:461	hyaluronan	452:461	hyaluronan due to its improved water solubility, which favours its use in designing new advanced composite hydrogels	452:567	It was found that WS/KL is highly compatible with hyaluronan due to its improved water solubility, which favours its use in designing new advanced composite hydrogels.
29253988	7	32	theme	KL	962:963	arg1	lignin					965:970	glycinated KL lignin	951:970	glycinated KL lignin	951:970	The cytotoxicity tests proved that glycinated KL lignin limits the viability of cells only slightly, and the final hyaluronan/lignin hydrogels were non-toxic materials.
29253988	4	33	theme	WS/KL	606:610	arg1	concentration					589:601	the concentration	585:601	the concentration of WS/KL	585:610	The effects of the concentration of WS/KL on morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels were investigated.
29253988	5	34	theme	%	835:835	arg1	incorporation					811:823	the incorporation	807:823	the incorporation of up to 3% (w/w) of WS/KL	807:850	It was detected that the creep resistance and creep recovery of NaHy hydrogels was improved by the incorporation of up to 3% (w/w) of WS/KL.
29253988	1	35	dep	stability	217:225	arg1	the					202:204	the	202:204	the	202:204	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	3	36	theme	advanced	540:547	arg1	hydrogels					559:567	new advanced composite hydrogels	536:567	new advanced composite hydrogels	536:567	It was found that WS/KL is highly compatible with hyaluronan due to its improved water solubility, which favours its use in designing new advanced composite hydrogels.
29253988	3	37	theme	due	463:465	arg1	hyaluronan					452:461	hyaluronan	452:461	hyaluronan due to its improved water solubility, which favours its use in designing new advanced composite hydrogels	452:567	It was found that WS/KL is highly compatible with hyaluronan due to its improved water solubility, which favours its use in designing new advanced composite hydrogels.
29253988	5	38	theme	NaHy	776:779	arg1	hydrogels					781:789	NaHy hydrogels	776:789	NaHy hydrogels	776:789	It was detected that the creep resistance and creep recovery of NaHy hydrogels was improved by the incorporation of up to 3% (w/w) of WS/KL.
29253988	6	39	theme	swelling	870:877	arg1	capacity					879:886	the swelling capacity	866:886	the swelling capacity of hydrogels	866:899	In contrast, the swelling capacity of hydrogels was decreased.
29253988	3	40	theme	composite	549:557	arg1	hydrogels					559:567	new advanced composite hydrogels	536:567	new advanced composite hydrogels	536:567	It was found that WS/KL is highly compatible with hyaluronan due to its improved water solubility, which favours its use in designing new advanced composite hydrogels.
29253988	4	41	theme	hydrogels	683:691	arg1	behaviours					658:667	morphological, swelling and creep/recovery behaviours	615:667	morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels	615:691	The effects of the concentration of WS/KL on morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels were investigated.
29253988	7	42	theme	glycinated	951:960	arg1	lignin					965:970	glycinated KL lignin	951:970	glycinated KL lignin	951:970	The cytotoxicity tests proved that glycinated KL lignin limits the viability of cells only slightly, and the final hyaluronan/lignin hydrogels were non-toxic materials.
29253988	2	43	theme	soluble	314:320	arg1	lignin					322:327	Water soluble lignin	308:327	Water soluble lignin	308:327	Water soluble lignin was obtained by the modification of Kraft lignin via a Mannich reaction.
29253988	1	44	theme	mechanical	206:215	arg1	stability					217:225	mechanical stability	206:225	mechanical stability	206:225	Effects of the addition of water soluble glycinated Kraft lignin (WS/KL) on the mechanical stability and biocompatibility of hyaluronan (NaHy) hydrogels were evaluated in this work.
29253988	2	45	theme	Water	308:312	arg1	lignin					322:327	Water soluble lignin	308:327	Water soluble lignin	308:327	Water soluble lignin was obtained by the modification of Kraft lignin via a Mannich reaction.
29253988	0	46	dep	hydrogels	11:19	arg1	modified					21:28	modified	21:28	hydrogels modified by glycinated Kraft lignin: Morphology, swelling, viscoelastic properties and biocompatibility	11:123	Hyaluronan hydrogels modified by glycinated Kraft lignin: Morphology, swelling, viscoelastic properties and biocompatibility.
29253988	3	47	theme	water	483:487	arg1	solubility					489:498	its improved water solubility	470:498	its improved water solubility	470:498	It was found that WS/KL is highly compatible with hyaluronan due to its improved water solubility, which favours its use in designing new advanced composite hydrogels.
29253988	2	48	theme	lignin	371:376	arg1	modification					349:360	the modification	345:360	the modification of Kraft lignin	345:376	Water soluble lignin was obtained by the modification of Kraft lignin via a Mannich reaction.
29253988	5	49	theme	creep	737:741	arg1	resistance					743:752	creep resistance	737:752	creep resistance	737:752	It was detected that the creep resistance and creep recovery of NaHy hydrogels was improved by the incorporation of up to 3% (w/w) of WS/KL.
29253988	4	50	theme	creep/recovery	643:656	arg1	behaviours					658:667	morphological, swelling and creep/recovery behaviours	615:667	morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels	615:691	The effects of the concentration of WS/KL on morphological, swelling and creep/recovery behaviours of hyaluronan hydrogels were investigated.
24231515	4	0	theme	hypotonic	773:781	arg1	lysis					794:798	lysis	794:798	lysis	794:798	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	4	0	theme	hypotonic	773:781	arg1	hemolysis					783:791	hypotonic hemolysis	773:791	hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells	773:936	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	1	1	theme	smaller	263:269	arg1	number					271:276	a smaller number	261:276	a smaller number of ASCs	261:284	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	7	2	theme	cell	1276:1279	arg1	yields					1281:1286	nucleated cell yields	1266:1286	nucleated cell yields	1266:1286	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	9	3	theme	non-woven	1788:1796	arg1	rayon					1798:1802	non-woven rayon	1788:1802	non-woven rayon	1788:1802	CONCLUSIONS Our results suggest that a column with non-woven rayon and polyethylene fabrics is useful for isolating stromal vascular fraction cells from LAF solutions for clinical applications.
24231515	3	4	theme	peripheral	550:559	arg1	PB					568:569	PB	568:569	PB	568:569	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	3	4	theme	peripheral	550:559	arg1	blood					561:565	peripheral blood	550:565	peripheral blood (PB)	550:570	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	8	5	theme	Mesenchymal	1582:1592	arg1	differentiation					1594:1608	Mesenchymal differentiation	1582:1608	Mesenchymal differentiation	1582:1608	Mesenchymal differentiation and network formation assays showed no statistical differences in ASC functions between the lysis and column-positive samples.
24231515	4	6	theme	blood	813:817	arg1	cell					819:822	the red blood cell	805:822	the red blood cell count	805:828	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	7	7	theme	LAF	1252:1254	arg1	solution					1256:1263	LAF solution	1252:1263	LAF solution	1252:1263	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	6	8	theme	negative	1188:1195	arg1	fraction					1197:1204	the negative fraction	1184:1204	the negative fraction	1184:1204	After column processing of PB-ASC, 60.5% of ASCs (53.2% by lysis) were selectively collected in the positive fraction, and the negative fraction contained almost no ASCs.
24231515	2	9	theme	LAF	520:522	arg1	cells					524:528	LAF cells	520:528	LAF cells	520:528	We evaluated the effectiveness of an adherent column, currently used to isolate mesenchymal stromal cells from bone marrow, to isolate LAF cells.
24231515	0	10	theme	adherent	127:134	arg1	column					136:141	an adherent column	124:141	an adherent column	124:141	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	5	11	theme	red	978:980	arg1	cells					988:992	red blood cells	978:992	red blood cells	978:992	RESULTS The column effectively removed red blood cells, though the removal efficiency was slightly inferior to hemolysis.
24231515	2	12	theme	adherent	422:429	arg1	column					431:436	an adherent column	419:436	an adherent column	419:436	We evaluated the effectiveness of an adherent column, currently used to isolate mesenchymal stromal cells from bone marrow, to isolate LAF cells.
24231515	8	13	theme	formation	1622:1630	arg1	assays					1632:1637	Mesenchymal differentiation and network formation assays	1582:1637	Mesenchymal differentiation and network formation assays	1582:1637	Mesenchymal differentiation and network formation assays showed no statistical differences in ASC functions between the lysis and column-positive samples.
24231515	7	14	theme	lysis	1477:1481	arg1	samples					1483:1489	the lysis samples	1473:1489	the lysis samples	1473:1489	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	6	15	theme	ASCs	1105:1108	arg1	ASCs					1105:1108	ASCs	1105:1108	ASCs (53.2% by lysis)	1105:1125	After column processing of PB-ASC, 60.5% of ASCs (53.2% by lysis) were selectively collected in the positive fraction, and the negative fraction contained almost no ASCs.
24231515	6	15	theme	ASCs	1105:1108	arg1	%					1100:1100	60.5%	1096:1100	60.5% of ASCs (53.2% by lysis)	1096:1125	After column processing of PB-ASC, 60.5% of ASCs (53.2% by lysis) were selectively collected in the positive fraction, and the negative fraction contained almost no ASCs.
24231515	9	16	theme	vascular	1861:1868	arg1	cells					1879:1883	stromal vascular fraction cells	1853:1883	stromal vascular fraction cells	1853:1883	CONCLUSIONS Our results suggest that a column with non-woven rayon and polyethylene fabrics is useful for isolating stromal vascular fraction cells from LAF solutions for clinical applications.
24231515	3	17	theme	non-adherent	709:720	arg1	fractions					733:741	the non-adherent (negative) fractions	705:741	the non-adherent (negative) fractions	705:741	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	3	17	theme	non-adherent	709:720	arg1	fractions					666:674	two fractions	662:674	two fractions	662:674	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	8	18	theme	statistical	1649:1659	arg1	differences					1661:1671	no statistical differences	1646:1671	no statistical differences in ASC functions between the lysis and column-positive samples	1646:1734	Mesenchymal differentiation and network formation assays showed no statistical differences in ASC functions between the lysis and column-positive samples.
24231515	4	19	theme	cell	857:860	arg1	compositions					862:873	cell compositions	857:873	cell compositions	857:873	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	6	20	dep	ASCs	1105:1108	arg1	%					1115:1115	53.2%	1111:1115	53.2%	1111:1115	After column processing of PB-ASC, 60.5% of ASCs (53.2% by lysis) were selectively collected in the positive fraction, and the negative fraction contained almost no ASCs.
24231515	0	21	theme	liquid	68:73	arg1	portion					75:81	the liquid portion	64:81	the liquid portion of liposuction aspirates with the use of an adherent column	64:141	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	5	22	theme	removal	1006:1012	arg1	efficiency					1014:1023	the removal efficiency	1002:1023	the removal efficiency	1002:1023	RESULTS The column effectively removed red blood cells, though the removal efficiency was slightly inferior to hemolysis.
24231515	5	22	theme	removal	1006:1012	arg1	inferior					1038:1045	inferior	1038:1045	inferior	1038:1045	RESULTS The column effectively removed red blood cells, though the removal efficiency was slightly inferior to hemolysis.
24231515	1	23	theme	BACKGROUND	144:153	arg1	ASCs					199:202	ASCs	199:202	ASCs	199:202	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	1	23	theme	BACKGROUND	144:153	arg1	cells					192:196	BACKGROUND AIMS Adipose-derived stem/progenitor cells	144:196	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs)	144:203	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	7	24	theme	viable	1569:1574	arg1	ASCs					1576:1579	viable ASCs	1569:1579	viable ASCs	1569:1579	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	2	25	theme	stromal	477:483	arg1	cells					485:489	mesenchymal stromal cells	465:489	mesenchymal stromal cells	465:489	We evaluated the effectiveness of an adherent column, currently used to isolate mesenchymal stromal cells from bone marrow, to isolate LAF cells.
24231515	9	26	theme	LAF	1890:1892	arg1	solutions					1894:1902	LAF solutions	1890:1902	LAF solutions for clinical applications	1890:1928	CONCLUSIONS Our results suggest that a column with non-woven rayon and polyethylene fabrics is useful for isolating stromal vascular fraction cells from LAF solutions for clinical applications.
24231515	1	27	theme	Adipose-derived	160:174	arg1	ASCs					199:202	ASCs	199:202	ASCs	199:202	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	1	27	theme	Adipose-derived	160:174	arg1	cells					192:196	BACKGROUND AIMS Adipose-derived stem/progenitor cells	144:196	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs)	144:203	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	4	28	theme	nucleated	831:839	arg1	cells					841:845	nucleated cells	831:845	nucleated cells count	831:851	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	3	29	theme	negative	723:730	arg1	fractions					733:741	the non-adherent (negative) fractions	705:741	the non-adherent (negative) fractions	705:741	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	3	29	theme	negative	723:730	arg1	fractions					666:674	two fractions	662:674	two fractions	662:674	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	0	30	theme	aspirates	98:106	arg1	portion					75:81	the liquid portion	64:81	the liquid portion of liposuction aspirates with the use of an adherent column	64:141	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	1	31	theme	liposuction	351:361	arg1	LAF					379:381	LAF	379:381	LAF	379:381	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	1	31	theme	liposuction	351:361	arg1	fluid					372:376	the infranatant liposuction aspirate fluid	335:376	the infranatant liposuction aspirate fluid (LAF)	335:382	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	3	32	dep	positive	691:698	arg1	the					677:679	the	677:679	the	677:679	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	4	33	theme	isolated	911:918	arg1	cells					932:936	isolated mesenchymal cells	911:936	isolated mesenchymal cells	911:936	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	0	34	theme	Enrichment	0:9	arg1	isolation					11:19	Enrichment isolation	0:19	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.	0:142	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	4	35	theme	cells	932:936	arg1	count					847:851	nucleated cells count	831:851	nucleated cells count	831:851	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	4	35	theme	cells	932:936	arg1	properties					897:906	functional properties	886:906	functional properties of isolated mesenchymal cells	886:936	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	4	35	theme	cells	932:936	arg1	compositions					862:873	cell compositions	857:873	cell compositions	857:873	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	4	35	theme	cells	932:936	arg1	count					824:828	the red blood cell count	805:828	the red blood cell count	805:828	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	1	36	theme	ASCs	281:284	arg1	number					271:276	a smaller number	261:276	a smaller number of ASCs	261:284	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	6	37	theme	column	1067:1072	arg1	processing					1074:1083	column processing	1067:1083	column processing of PB-ASC	1067:1093	After column processing of PB-ASC, 60.5% of ASCs (53.2% by lysis) were selectively collected in the positive fraction, and the negative fraction contained almost no ASCs.
24231515	7	38	theme	higher	1392:1397	arg1	yield					1411:1415	a higher average ASC yield	1390:1415	a higher average ASC yield	1390:1415	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	0	39	theme	stem/stromal	40:51	arg1	cells					53:57	adipose-derived stem/stromal cells	24:57	adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column	24:141	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	7	40	dep	column	1316:1321	arg1	the					1312:1314	the	1312:1314	the	1312:1314	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	3	41	theme	LAF	615:617	arg1	solution					619:626	LAF solution	615:626	LAF solution	615:626	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	7	42	theme	subsequent	1347:1356	arg1	culture					1367:1373	subsequent adherent culture	1347:1373	subsequent adherent culture	1347:1373	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	9	43	with	column	1776:1781	arg1	rayon					1798:1802	non-woven rayon	1788:1802	non-woven rayon	1788:1802	CONCLUSIONS Our results suggest that a column with non-woven rayon and polyethylene fabrics is useful for isolating stromal vascular fraction cells from LAF solutions for clinical applications.
24231515	9	43	with	column	1776:1781	arg1	fabrics					1821:1827	polyethylene fabrics	1808:1827	polyethylene fabrics	1808:1827	CONCLUSIONS Our results suggest that a column with non-woven rayon and polyethylene fabrics is useful for isolating stromal vascular fraction cells from LAF solutions for clinical applications.
24231515	0	44	link	adipose-derived	24:38	arg1	cells					53:57	adipose-derived stem/stromal cells	24:57	adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column	24:141	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	5	45	dep	RESULTS	939:945	arg1	removed					970:976	removed	970:976	removed	970:976	RESULTS The column effectively removed red blood cells, though the removal efficiency was slightly inferior to hemolysis.
24231515	1	46	theme	stem/progenitor	176:190	arg1	ASCs					199:202	ASCs	199:202	ASCs	199:202	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	1	46	theme	stem/progenitor	176:190	arg1	cells					192:196	BACKGROUND AIMS Adipose-derived stem/progenitor cells	144:196	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs)	144:203	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	6	47	contain	contained	1206:1214	arg2	ASCs					1226:1229	almost no ASCs	1216:1229	almost no ASCs	1216:1229	After column processing of PB-ASC, 60.5% of ASCs (53.2% by lysis) were selectively collected in the positive fraction, and the negative fraction contained almost no ASCs.
24231515	6	47	contain	contained	1206:1214	arg1	fraction					1197:1204	the negative fraction	1184:1204	the negative fraction	1184:1204	After column processing of PB-ASC, 60.5% of ASCs (53.2% by lysis) were selectively collected in the positive fraction, and the negative fraction contained almost no ASCs.
24231515	7	48	theme	nucleated	1266:1274	arg1	yields					1281:1286	nucleated cell yields	1266:1286	nucleated cell yields	1266:1286	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	9	49	theme	polyethylene	1808:1819	arg1	fabrics					1821:1827	polyethylene fabrics	1808:1827	polyethylene fabrics	1808:1827	CONCLUSIONS Our results suggest that a column with non-woven rayon and polyethylene fabrics is useful for isolating stromal vascular fraction cells from LAF solutions for clinical applications.
24231515	0	50	with	aspirates	98:106	arg1	use					117:119	the use	113:119	the use of an adherent column	113:141	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	4	51	theme	red	809:811	arg1	cell					819:822	the red blood cell	805:822	the red blood cell count	805:828	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	7	52	theme	solution	1256:1263	arg1	processing					1238:1247	processing	1238:1247	processing of LAF solution	1238:1263	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	8	53	from	differences	1661:1671	arg1	functions					1680:1688	ASC functions	1676:1688	ASC functions	1676:1688	Mesenchymal differentiation and network formation assays showed no statistical differences in ASC functions between the lysis and column-positive samples.
24231515	0	54	theme	column	136:141	arg1	use					117:119	the use	113:119	the use of an adherent column	113:141	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	8	55	theme	differentiation	1594:1608	arg1	assays					1632:1637	Mesenchymal differentiation and network formation assays	1582:1637	Mesenchymal differentiation and network formation assays	1582:1637	Mesenchymal differentiation and network formation assays showed no statistical differences in ASC functions between the lysis and column-positive samples.
24231515	4	56	theme	cell	819:822	arg1	count					824:828	the red blood cell count	805:828	the red blood cell count	805:828	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	0	57	from	portion	75:81	arg1	cells					53:57	adipose-derived stem/stromal cells	24:57	adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column	24:141	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	0	57	from	portion	75:81	arg1	isolation					11:19	Enrichment isolation	0:19	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.	0:142	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	5	58	theme	blood	982:986	arg1	cells					988:992	red blood cells	978:992	red blood cells	978:992	RESULTS The column effectively removed red blood cells, though the removal efficiency was slightly inferior to hemolysis.
24231515	8	59	theme	network	1614:1620	arg1	formation					1622:1630	network formation	1614:1630	network formation	1614:1630	Mesenchymal differentiation and network formation assays showed no statistical differences in ASC functions between the lysis and column-positive samples.
24231515	9	60	theme	stromal	1853:1859	arg1	cells					1879:1883	stromal vascular fraction cells	1853:1883	stromal vascular fraction cells	1853:1883	CONCLUSIONS Our results suggest that a column with non-woven rayon and polyethylene fabrics is useful for isolating stromal vascular fraction cells from LAF solutions for clinical applications.
24231515	6	61	theme	positive	1161:1168	arg1	fraction					1170:1177	the positive fraction	1157:1177	the positive fraction	1157:1177	After column processing of PB-ASC, 60.5% of ASCs (53.2% by lysis) were selectively collected in the positive fraction, and the negative fraction contained almost no ASCs.
24231515	7	62	theme	column-positive	1439:1453	arg1	samples					1455:1461	the column-positive samples	1435:1461	the column-positive samples than from the lysis samples	1435:1489	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	9	63	theme	fraction	1870:1877	arg1	cells					1879:1883	stromal vascular fraction cells	1853:1883	stromal vascular fraction cells	1853:1883	CONCLUSIONS Our results suggest that a column with non-woven rayon and polyethylene fabrics is useful for isolating stromal vascular fraction cells from LAF solutions for clinical applications.
24231515	3	64	dep	METHODS	531:537	arg1	divided					646:652	divided	646:652	divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions	646:741	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	3	64	dep	METHODS	531:537	arg1	applied					542:548	applied	542:548	applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column	542:640	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	6	65	theme	PB-ASC	1088:1093	arg1	processing					1074:1083	column processing	1067:1083	column processing of PB-ASC	1067:1093	After column processing of PB-ASC, 60.5% of ASCs (53.2% by lysis) were selectively collected in the positive fraction, and the negative fraction contained almost no ASCs.
24231515	6	66	theme	no	1223:1224	arg1	ASCs					1226:1229	almost no ASCs	1216:1229	almost no ASCs	1216:1229	After column processing of PB-ASC, 60.5% of ASCs (53.2% by lysis) were selectively collected in the positive fraction, and the negative fraction contained almost no ASCs.
24231515	0	67	theme	liposuction	86:96	arg1	aspirates					98:106	liposuction aspirates	86:106	liposuction aspirates with the use of an adherent column	86:141	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	1	68	theme	AIMS	155:158	arg1	ASCs					199:202	ASCs	199:202	ASCs	199:202	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	1	68	theme	AIMS	155:158	arg1	cells					192:196	BACKGROUND AIMS Adipose-derived stem/progenitor cells	144:196	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs)	144:203	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	2	69	theme	mesenchymal	465:475	arg1	cells					485:489	mesenchymal stromal cells	465:489	mesenchymal stromal cells	465:489	We evaluated the effectiveness of an adherent column, currently used to isolate mesenchymal stromal cells from bone marrow, to isolate LAF cells.
24231515	0	70	from	isolation	11:19	arg1	portion					75:81	the liquid portion	64:81	the liquid portion of liposuction aspirates with the use of an adherent column	64:141	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	1	71	theme	infranatant	339:349	arg1	LAF					379:381	LAF	379:381	LAF	379:381	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	1	71	theme	infranatant	339:349	arg1	fluid					372:376	the infranatant liposuction aspirate fluid	335:376	the infranatant liposuction aspirate fluid (LAF)	335:382	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	8	72	theme	ASC	1676:1678	arg1	functions					1680:1688	ASC functions	1676:1688	ASC functions	1676:1688	Mesenchymal differentiation and network formation assays showed no statistical differences in ASC functions between the lysis and column-positive samples.
24231515	7	73	theme	ASC	1407:1409	arg1	yield					1411:1415	a higher average ASC yield	1390:1415	a higher average ASC yield	1390:1415	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	9	74	theme	clinical	1908:1915	arg1	applications					1917:1928	clinical applications	1908:1928	clinical applications	1908:1928	CONCLUSIONS Our results suggest that a column with non-woven rayon and polyethylene fabrics is useful for isolating stromal vascular fraction cells from LAF solutions for clinical applications.
24231515	1	75	theme	aspirate	363:370	arg1	LAF					379:381	LAF	379:381	LAF	379:381	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	1	75	theme	aspirate	363:370	arg1	fluid					372:376	the infranatant liposuction aspirate fluid	335:376	the infranatant liposuction aspirate fluid (LAF)	335:382	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	7	76	from	samples	1483:1489	arg1	samples					1455:1461	the column-positive samples	1435:1461	the column-positive samples than from the lysis samples	1435:1489	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	9	77	dep	CONCLUSIONS	1737:1747	arg1	suggest					1761:1767	suggest	1761:1767	suggest that a column with non-woven rayon and polyethylene fabrics is useful for isolating stromal vascular fraction cells from LAF solutions for clinical applications	1761:1928	CONCLUSIONS Our results suggest that a column with non-woven rayon and polyethylene fabrics is useful for isolating stromal vascular fraction cells from LAF solutions for clinical applications.
24231515	2	78	theme	bone	496:499	arg1	marrow					501:506	bone marrow	496:506	bone marrow	496:506	We evaluated the effectiveness of an adherent column, currently used to isolate mesenchymal stromal cells from bone marrow, to isolate LAF cells.
24231515	1	79	from	fluid	372:376	arg1	digestion					320:328	enzymatic digestion	310:328	enzymatic digestion from the infranatant liposuction aspirate fluid (LAF)	310:382	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	8	80	theme	lysis	1702:1706	arg1	samples					1728:1734	the lysis and column-positive samples	1698:1734	samples	1728:1734	Mesenchymal differentiation and network formation assays showed no statistical differences in ASC functions between the lysis and column-positive samples.
24231515	2	81	theme	column	431:436	arg1	effectiveness					402:414	the effectiveness	398:414	the effectiveness of an adherent column, currently used to isolate mesenchymal stromal cells from bone marrow, to isolate LAF cells	398:528	We evaluated the effectiveness of an adherent column, currently used to isolate mesenchymal stromal cells from bone marrow, to isolate LAF cells.
24231515	0	82	theme	adipose-derived	24:38	arg1	cells					53:57	adipose-derived stem/stromal cells	24:57	adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column	24:141	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	8	83	theme	column-positive	1712:1726	arg1	samples					1728:1734	the lysis and column-positive samples	1698:1734	samples	1728:1734	Mesenchymal differentiation and network formation assays showed no statistical differences in ASC functions between the lysis and column-positive samples.
24231515	4	84	theme	mesenchymal	920:930	arg1	cells					932:936	isolated mesenchymal cells	911:936	isolated mesenchymal cells	911:936	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	1	85	link	Adipose-derived	160:174	arg1	ASCs					199:202	ASCs	199:202	ASCs	199:202	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	1	85	link	Adipose-derived	160:174	arg1	cells					192:196	BACKGROUND AIMS Adipose-derived stem/progenitor cells	144:196	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs)	144:203	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	0	86	theme	cells	53:57	arg1	isolation					11:19	Enrichment isolation	0:19	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.	0:142	Enrichment isolation of adipose-derived stem/stromal cells from the liquid portion of liposuction aspirates with the use of an adherent column.
24231515	7	87	theme	average	1399:1405	arg1	yield					1411:1415	a higher average ASC yield	1390:1415	a higher average ASC yield	1390:1415	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	7	88	theme	column	1512:1517	arg1	method					1519:1524	the column method	1508:1524	the column method	1508:1524	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	7	88	theme	column	1512:1517	arg1	superior					1533:1540	superior	1533:1540	superior	1533:1540	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
24231515	3	89	theme	cultured	587:594	arg1	PB-ASC					602:607	PB-ASC	602:607	PB-ASC	602:607	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	3	89	theme	cultured	587:594	arg1	ASCs					596:599	cultured ASCs	587:599	cultured ASCs (PB-ASC)	587:608	METHODS We applied peripheral blood (PB), PB mixed with cultured ASCs (PB-ASC), and LAF solution to the column and divided it into two fractions, the adherent (positive) and the non-adherent (negative) fractions.
24231515	4	90	theme	cells	841:845	arg1	count					847:851	nucleated cells count	831:851	nucleated cells count	831:851	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	4	91	theme	functional	886:895	arg1	properties					897:906	functional properties	886:906	functional properties of isolated mesenchymal cells	886:936	We compared this method with hypotonic hemolysis (lysis) for the red blood cell count, nucleated cells count and cell compositions as well as functional properties of isolated mesenchymal cells.
24231515	1	92	theme	enzymatic	310:318	arg1	digestion					320:328	enzymatic digestion	310:328	enzymatic digestion from the infranatant liposuction aspirate fluid (LAF)	310:382	BACKGROUND AIMS Adipose-derived stem/progenitor cells (ASCs) are typically obtained from the lipoaspirates; however, a smaller number of ASCs can be isolated without enzymatic digestion from the infranatant liposuction aspirate fluid (LAF).
24231515	7	93	theme	adherent	1358:1365	arg1	culture					1367:1373	subsequent adherent culture	1347:1373	subsequent adherent culture	1347:1373	After processing of LAF solution, nucleated cell yields were comparable between the column and hemolysis; however, subsequent adherent culture indicated that a higher average ASC yield was obtained from the column-positive samples than from the lysis samples, suggesting that the column method may be superior to hemolysis for obtaining viable ASCs.
28918133	12	0	theme	components	2021:2030	arg1	yield					1999:2003	yield	1999:2003	yield	1999:2003	Treatments did not affect DMI or yield of milk or milk components in the first experiment.
28918133	12	0	theme	components	2021:2030	arg1	DMI					1992:1994	DMI	1992:1994	DMI	1992:1994	Treatments did not affect DMI or yield of milk or milk components in the first experiment.
28918133	15	1	theme	LM	2463:2464	arg1	treatments					2466:2475	the all, LH, and LM treatments	2446:2475	the all, LH, and LM treatments	2446:2475	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	10	2	theme	0.9	1578:1580	arg1	%					1581:1581	%	1581:1581	%	1581:1581	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	5	3	dep	%	846:846	arg1	high					849:852	high	849:852	high	849:852	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	17	4	theme	limiting	2822:2829	arg1	AA					2831:2832	other limiting AA	2816:2832	other limiting AA	2816:2832	This may be due to our hypothesis being inaccurate or to other factors; other limiting AA could have prevented the effects of Lys, Met, and His infusions or the infusion periods could have been too short to induce a response in DMI.
28918133	18	5	theme	sensitive	3034:3042	arg1	method					3044:3049	a sensitive method	3032:3049	a sensitive method to detect limiting AA in dairy cow diets	3032:3090	Accordingly, short-term infusion of AA is probably not a sensitive method to detect limiting AA in dairy cow diets.
28918133	18	5	theme	sensitive	3034:3042	arg1	infusion					3001:3008	short-term infusion	2990:3008	short-term infusion of AA	2990:3014	Accordingly, short-term infusion of AA is probably not a sensitive method to detect limiting AA in dairy cow diets.
28918133	4	6	used	used	641:644	arg2	experiment					630:639	The first experiment	620:639	The first experiment	620:639	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	14	7	theme	third	2219:2223	arg1	experiment					2225:2234	the third experiment	2215:2234	the third experiment	2215:2234	In the third experiment, the AA treatment tended to increase yields of milk, lactose, and protein as well as MUN concentration but did not affect DMI.
28918133	2	8	theme	cow	500:502	arg1	diets					504:508	dairy cow diets	494:508	dairy cow diets	494:508	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	9	9	theme	d	1431:1431	arg1	PP					1433:1434	4-14 d PP	1426:1434	4-14 d PP	1426:1434	In the fourth experiment, 5 cows (4-14 d PP) were used in a 5 × 5 Latin square design.
28918133	9	9	theme	d	1431:1431	arg1	cows					1420:1423	5 cows	1418:1423	5 cows (4-14 d PP)	1418:1435	In the fourth experiment, 5 cows (4-14 d PP) were used in a 5 × 5 Latin square design.
28918133	17	10	dep	Lys	2870:2872	arg1	infusions					2888:2896	infusions	2888:2896	infusions	2888:2896	This may be due to our hypothesis being inaccurate or to other factors; other limiting AA could have prevented the effects of Lys, Met, and His infusions or the infusion periods could have been too short to induce a response in DMI.
28918133	8	11	theme	requirement	1314:1324	arg1	%					1294:1294	15%	1292:1294	15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT)	1292:1389	Treatments were 15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT).
28918133	8	11	theme	requirement	1314:1324	arg1	requirement					1314:1324	the calculated requirement	1299:1324	the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT)	1299:1389	Treatments were 15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT).
28918133	12	12	from	yield	1999:2003	arg1	experiment					2045:2054	the first experiment	2035:2054	the first experiment	2035:2054	Treatments did not affect DMI or yield of milk or milk components in the first experiment.
28918133	16	13	theme	short-term	2672:2681	arg1	addition					2683:2690	short-term addition	2672:2690	short-term addition of these potentially limiting AA	2672:2723	These results failed to provide support for our hypothesis that short-term addition of these potentially limiting AA will increase DMI.
28918133	4	14	theme	square	696:701	arg1	design					703:708	a 4 × 4 Latin square design	682:708	a 4 × 4 Latin square design	682:708	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	13	15	contain	had	2160:2162	arg1	treatment					2086:2094	AA treatment	2083:2094	AA treatment	2083:2094	In the second experiment, AA treatment increased protein percentage and reduced lactose percentage but had no effect on protein and lactose yields or DMI.
28918133	13	15	contain	had	2160:2162	arg2	effect					2167:2172	no effect	2164:2172	no effect	2164:2172	In the second experiment, AA treatment increased protein percentage and reduced lactose percentage but had no effect on protein and lactose yields or DMI.
28918133	15	16	dep	all	2450:2452	arg1	the					2446:2448	the	2446:2448	the	2446:2448	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	8	17	theme	metabolizable	1329:1341	arg1	Met					1343:1345	metabolizable Met	1329:1345	metabolizable Met	1329:1345	Treatments were 15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT).
28918133	1	18	theme	hepatic	242:248	arg1	oxidation					250:258	hepatic oxidation	242:258	hepatic oxidation	242:258	We hypothesized that the addition of limiting AA increases dry matter intake (DMI) by reducing anaplerosis and hepatic oxidation.
28918133	13	19	dep	protein	2177:2183	arg1	yields					2197:2202	yields	2197:2202	yields	2197:2202	In the second experiment, AA treatment increased protein percentage and reduced lactose percentage but had no effect on protein and lactose yields or DMI.
28918133	6	20	theme	third	970:974	arg1	experiments					976:986	the second and third experiments	955:986	the second and third experiments	955:986	In the second and third experiments, 8 cows (4-12 d PP) were divided into 2 groups of 4 cows, and each group received a different diet formulated to either be low in Lys (experiment 2) or Met (experiment 3).
28918133	18	21	theme	limiting	3061:3068	arg1	AA					3070:3071	limiting AA	3061:3071	limiting AA	3061:3071	Accordingly, short-term infusion of AA is probably not a sensitive method to detect limiting AA in dairy cow diets.
28918133	14	22	theme	MUN	2321:2323	arg1	concentration					2325:2337	MUN concentration	2321:2337	yields of milk, lactose, and protein as well as MUN concentration	2273:2337	In the third experiment, the AA treatment tended to increase yields of milk, lactose, and protein as well as MUN concentration but did not affect DMI.
28918133	1	23	theme	limiting	168:175	arg1	AA					177:178	limiting AA	168:178	limiting AA	168:178	We hypothesized that the addition of limiting AA increases dry matter intake (DMI) by reducing anaplerosis and hepatic oxidation.
28918133	2	24	theme	specific	466:473	arg1	AA					475:476	specific AA	466:476	specific AA	466:476	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	2	25	from	effects	320:326	arg1	DMI					432:434	DMI	432:434	DMI	432:434	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	9	26	theme	×	1454:1454	arg1	design					1471:1476	a 5 × 5 Latin square design	1450:1476	a 5 × 5 Latin square design	1450:1476	In the fourth experiment, 5 cows (4-14 d PP) were used in a 5 × 5 Latin square design.
28918133	18	27	theme	AA	3013:3014	arg1	infusion					3001:3008	short-term infusion	2990:3008	short-term infusion of AA	2990:3014	Accordingly, short-term infusion of AA is probably not a sensitive method to detect limiting AA in dairy cow diets.
28918133	18	27	theme	AA	3013:3014	arg1	method					3044:3049	a sensitive method	3032:3049	a sensitive method to detect limiting AA in dairy cow diets	3032:3090	Accordingly, short-term infusion of AA is probably not a sensitive method to detect limiting AA in dairy cow diets.
28918133	0	28	theme	cattle	117:122	arg1	diets					124:128	dairy cattle diets	111:128	dairy cattle diets	111:128	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.
28918133	1	29	theme	matter	194:199	arg1	DMI					209:211	DMI	209:211	DMI	209:211	We hypothesized that the addition of limiting AA increases dry matter intake (DMI) by reducing anaplerosis and hepatic oxidation.
28918133	1	29	theme	matter	194:199	arg1	intake					201:206	dry matter intake	190:206	dry matter intake (DMI)	190:212	We hypothesized that the addition of limiting AA increases dry matter intake (DMI) by reducing anaplerosis and hepatic oxidation.
28918133	12	30	from	DMI	1992:1994	arg1	experiment					2045:2054	the first experiment	2035:2054	the first experiment	2035:2054	Treatments did not affect DMI or yield of milk or milk components in the first experiment.
28918133	9	31	theme	Latin	1458:1462	arg1	design					1471:1476	a 5 × 5 Latin square design	1450:1476	a 5 × 5 Latin square design	1450:1476	In the fourth experiment, 5 cows (4-14 d PP) were used in a 5 × 5 Latin square design.
28918133	5	32	dep	5	819:819	arg1	low					822:824	low	822:824	low	822:824	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	17	33	theme	other	2801:2805	arg1	factors					2807:2813	other factors	2801:2813	other factors	2801:2813	This may be due to our hypothesis being inaccurate or to other factors; other limiting AA could have prevented the effects of Lys, Met, and His infusions or the infusion periods could have been too short to induce a response in DMI.
28918133	13	34	theme	reduced	2129:2135	arg1	percentage					2145:2154	reduced lactose percentage	2129:2154	reduced lactose percentage	2129:2154	In the second experiment, AA treatment increased protein percentage and reduced lactose percentage but had no effect on protein and lactose yields or DMI.
28918133	4	35	dep	cows	648:651	arg1	PP					675:676	PP	675:676	PP	675:676	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	4	35	dep	cows	648:651	arg1	postpartum					663:672	d postpartum	661:672	4 cows 6 to 10 d postpartum (PP)	646:677	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	17	36	theme	infusion	2905:2912	arg1	short					2942:2946	short	2942:2946	short	2942:2946	This may be due to our hypothesis being inaccurate or to other factors; other limiting AA could have prevented the effects of Lys, Met, and His infusions or the infusion periods could have been too short to induce a response in DMI.
28918133	17	36	theme	infusion	2905:2912	arg1	periods					2914:2920	the infusion periods	2901:2920	the infusion periods	2901:2920	This may be due to our hypothesis being inaccurate or to other factors; other limiting AA could have prevented the effects of Lys, Met, and His infusions or the infusion periods could have been too short to induce a response in DMI.
28918133	5	37	theme	%	846:846	arg1	infusions					806:814	continuous infusions	795:814	continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT)	795:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	13	38	theme	AA	2083:2084	arg1	treatment					2086:2094	AA treatment	2083:2094	AA treatment	2083:2094	In the second experiment, AA treatment increased protein percentage and reduced lactose percentage but had no effect on protein and lactose yields or DMI.
28918133	17	39	theme	Lys	2870:2872	arg1	effects					2859:2865	the effects	2855:2865	the effects of Lys, Met, and His infusions	2855:2896	This may be due to our hypothesis being inaccurate or to other factors; other limiting AA could have prevented the effects of Lys, Met, and His infusions or the infusion periods could have been too short to induce a response in DMI.
28918133	15	40	theme	other	2590:2594	arg1	treatments					2596:2605	other treatments	2590:2605	other treatments	2590:2605	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	7	41	theme	12-h	1225:1228	arg1	infusions					1230:1238	12-h infusions	1225:1238	12-h infusions (continuous)	1225:1251	Each experiment was a crossover design with two 1-d periods with 12-h infusions (continuous) and 12 h for recovery.
28918133	5	42	theme	Met	902:904	arg1	saline					928:933	0.9% saline	923:933	0.9% saline (control, CONT)	923:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	5	42	theme	Met	902:904	arg1	requirement					873:883	the calculated requirement	858:883	the calculated requirement of metabolizable Met, Lys, and His	858:918	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	8	43	theme	0.9	1372:1374	arg1	%					1375:1375	%	1375:1375	%	1375:1375	Treatments were 15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT).
28918133	17	44	theme	Met	2875:2877	arg1	effects					2859:2865	the effects	2855:2865	the effects of Lys, Met, and His infusions	2855:2896	This may be due to our hypothesis being inaccurate or to other factors; other limiting AA could have prevented the effects of Lys, Met, and His infusions or the infusion periods could have been too short to induce a response in DMI.
28918133	15	45	theme	fourth	2370:2375	arg1	experiment					2377:2386	the fourth experiment	2366:2386	the fourth experiment	2366:2386	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	11	46	theme	computerized	1797:1808	arg1	system					1827:1832	a computerized data acquisition system	1795:1832	a computerized data acquisition system	1795:1832	In each experiment, feed intake was recorded by a computerized data acquisition system, milk yield was recorded, and milk composition was analyzed for fat, protein, lactose, and milk urea nitrogen (MUN) concentrations.
28918133	11	47	theme	feed	1767:1770	arg1	intake					1772:1777	feed intake	1767:1777	feed intake	1767:1777	In each experiment, feed intake was recorded by a computerized data acquisition system, milk yield was recorded, and milk composition was analyzed for fat, protein, lactose, and milk urea nitrogen (MUN) concentrations.
28918133	16	48	theme	AA	2722:2723	arg1	addition					2683:2690	short-term addition	2672:2690	short-term addition of these potentially limiting AA	2672:2723	These results failed to provide support for our hypothesis that short-term addition of these potentially limiting AA will increase DMI.
28918133	14	49	theme	lactose	2289:2295	arg1	yields					2273:2278	yields	2273:2278	yields of milk, lactose, and protein as well as MUN concentration	2273:2337	In the third experiment, the AA treatment tended to increase yields of milk, lactose, and protein as well as MUN concentration but did not affect DMI.
28918133	14	49	theme	lactose	2289:2295	arg1	concentration					2325:2337	MUN concentration	2321:2337	yields of milk, lactose, and protein as well as MUN concentration	2273:2337	In the third experiment, the AA treatment tended to increase yields of milk, lactose, and protein as well as MUN concentration but did not affect DMI.
28918133	5	50	theme	Lys	907:909	arg1	saline					928:933	0.9% saline	923:933	0.9% saline (control, CONT)	923:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	5	50	theme	Lys	907:909	arg1	requirement					873:883	the calculated requirement	858:883	the calculated requirement of metabolizable Met, Lys, and His	858:918	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	8	51	theme	saline	1377:1382	arg1	requirement					1314:1324	the calculated requirement	1299:1324	the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT)	1299:1389	Treatments were 15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT).
28918133	5	52	dep	saline	928:933	arg1	CONT					945:948	CONT	945:948	CONT	945:948	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	5	52	dep	saline	928:933	arg1	control					936:942	control	936:942	control	936:942	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	13	53	theme	second	2064:2069	arg1	experiment					2071:2080	the second experiment	2060:2080	the second experiment	2060:2080	In the second experiment, AA treatment increased protein percentage and reduced lactose percentage but had no effect on protein and lactose yields or DMI.
28918133	8	54	dep	Lys	1348:1350	arg1	high					1362:1365	high	1362:1365	high	1362:1365	Treatments were 15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT).
28918133	14	55	theme	milk	2283:2286	arg1	yields					2273:2278	yields	2273:2278	yields of milk, lactose, and protein as well as MUN concentration	2273:2337	In the third experiment, the AA treatment tended to increase yields of milk, lactose, and protein as well as MUN concentration but did not affect DMI.
28918133	14	55	theme	milk	2283:2286	arg1	concentration					2325:2337	MUN concentration	2321:2337	yields of milk, lactose, and protein as well as MUN concentration	2273:2337	In the third experiment, the AA treatment tended to increase yields of milk, lactose, and protein as well as MUN concentration but did not affect DMI.
28918133	7	56	dep	infusions	1230:1238	arg1	continuous					1241:1250	continuous	1241:1250	continuous	1241:1250	Each experiment was a crossover design with two 1-d periods with 12-h infusions (continuous) and 12 h for recovery.
28918133	18	57	theme	cow	3082:3084	arg1	diets					3086:3090	dairy cow diets	3076:3090	dairy cow diets	3076:3090	Accordingly, short-term infusion of AA is probably not a sensitive method to detect limiting AA in dairy cow diets.
28918133	18	58	theme	dairy	3076:3080	arg1	diets					3086:3090	dairy cow diets	3076:3090	dairy cow diets	3076:3090	Accordingly, short-term infusion of AA is probably not a sensitive method to detect limiting AA in dairy cow diets.
28918133	0	59	theme	amino	96:100	arg1	acids					102:106	limiting amino acids	87:106	limiting amino acids	87:106	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.
28918133	14	60	theme	AA	2241:2242	arg1	treatment					2244:2252	the AA treatment	2237:2252	the AA treatment	2237:2252	In the third experiment, the AA treatment tended to increase yields of milk, lactose, and protein as well as MUN concentration but did not affect DMI.
28918133	10	61	dep	LM	1623:1624	arg1	Met					1635:1637	Met	1635:1637	Met	1635:1637	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	10	61	dep	LM	1623:1624	arg1	Lys					1627:1629	Lys	1627:1629	Lys	1627:1629	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	11	62	theme	acquisition	1815:1825	arg1	system					1827:1832	a computerized data acquisition system	1795:1832	a computerized data acquisition system	1795:1832	In each experiment, feed intake was recorded by a computerized data acquisition system, milk yield was recorded, and milk composition was analyzed for fat, protein, lactose, and milk urea nitrogen (MUN) concentrations.
28918133	2	63	theme	intravenous	342:352	arg1	infusions					354:362	short-term intravenous infusions	331:362	short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA)	331:427	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	0	64	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.	0:129	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.
28918133	5	65	theme	%	926:926	arg1	saline					928:933	0.9% saline	923:933	0.9% saline (control, CONT)	923:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	7	66	with	h	1260:1260	arg1	periods					1212:1218	two 1-d periods	1204:1218	two 1-d periods with 12-h infusions (continuous)	1204:1251	Each experiment was a crossover design with two 1-d periods with 12-h infusions (continuous) and 12 h for recovery.
28918133	2	67	theme	His	381:383	arg1	infusions					354:362	short-term intravenous infusions	331:362	short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA)	331:427	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	11	68	theme	urea	1930:1933	arg1	MUN					1945:1947	MUN	1945:1947	MUN	1945:1947	In each experiment, feed intake was recorded by a computerized data acquisition system, milk yield was recorded, and milk composition was analyzed for fat, protein, lactose, and milk urea nitrogen (MUN) concentrations.
28918133	11	68	theme	urea	1930:1933	arg1	nitrogen					1935:1942	milk urea nitrogen	1925:1942	milk urea nitrogen (MUN) concentrations	1925:1963	In each experiment, feed intake was recorded by a computerized data acquisition system, milk yield was recorded, and milk composition was analyzed for fat, protein, lactose, and milk urea nitrogen (MUN) concentrations.
28918133	5	69	theme	continuous	795:804	arg1	infusions					806:814	continuous infusions	795:814	continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT)	795:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	0	70	theme	intravenous	32:42	arg1	infusions					55:63	Short-term intravenous amino acid infusions	21:63	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.	0:129	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.
28918133	10	71	dep	all	1598:1600	arg1	Met					1608:1610	Met	1608:1610	Met	1608:1610	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	10	71	dep	all	1598:1600	arg1	His					1617:1619	His	1617:1619	His	1617:1619	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	10	71	dep	all	1598:1600	arg1	Lys					1603:1605	Lys	1603:1605	Lys	1603:1605	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	3	72	theme	Holstein	544:551	arg1	cows					553:556	Holstein cows	544:556	Holstein cows	544:556	We conducted 4 experiments using Holstein cows in the immediate postpartum period to address this objective.
28918133	0	73	theme	acid	50:53	arg1	infusions					55:63	Short-term intravenous amino acid infusions	21:63	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.	0:129	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.
28918133	15	74	theme	milk	2485:2488	arg1	concentration					2498:2510	milk lactose concentration	2485:2510	milk lactose concentration	2485:2510	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	9	75	theme	fourth	1399:1404	arg1	experiment					1406:1415	the fourth experiment	1395:1415	the fourth experiment	1395:1415	In the fourth experiment, 5 cows (4-14 d PP) were used in a 5 × 5 Latin square design.
28918133	2	76	theme	work	296:299	arg1	objective					278:286	the objective	274:286	the objective of this work	274:299	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	6	77	from	Lys	1118:1120	arg1	low					1111:1113	low	1111:1113	low	1111:1113	In the second and third experiments, 8 cows (4-12 d PP) were divided into 2 groups of 4 cows, and each group received a different diet formulated to either be low in Lys (experiment 2) or Met (experiment 3).
28918133	10	78	theme	first	1550:1554	arg1	Treatments					1562:1571	the first 46 h. Treatments	1546:1571	the first 46 h. Treatments	1546:1571	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	3	79	theme	immediate	565:573	arg1	period					586:591	the immediate postpartum period	561:591	the immediate postpartum period	561:591	We conducted 4 experiments using Holstein cows in the immediate postpartum period to address this objective.
28918133	15	80	theme	LH	2455:2456	arg1	treatments					2466:2475	the all, LH, and LM treatments	2446:2475	the all, LH, and LM treatments	2446:2475	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	5	81	theme	10	828:829	arg1	infusions					806:814	continuous infusions	795:814	continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT)	795:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	10	82	theme	%	1581:1581	arg1	CONT					1591:1594	CONT	1591:1594	CONT	1591:1594	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	10	82	theme	%	1581:1581	arg1	saline					1583:1588	0.9% saline	1578:1588	0.9% saline (CONT)	1578:1595	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	17	83	theme	other	2816:2820	arg1	AA					2831:2832	other limiting AA	2816:2832	other limiting AA	2816:2832	This may be due to our hypothesis being inaccurate or to other factors; other limiting AA could have prevented the effects of Lys, Met, and His infusions or the infusion periods could have been too short to induce a response in DMI.
28918133	6	84	theme	d	1002:1002	arg1	PP					1004:1005	4-12 d PP	997:1005	4-12 d PP	997:1005	In the second and third experiments, 8 cows (4-12 d PP) were divided into 2 groups of 4 cows, and each group received a different diet formulated to either be low in Lys (experiment 2) or Met (experiment 3).
28918133	6	84	theme	d	1002:1002	arg1	cows					991:994	8 cows	989:994	8 cows (4-12 d PP)	989:1006	In the second and third experiments, 8 cows (4-12 d PP) were divided into 2 groups of 4 cows, and each group received a different diet formulated to either be low in Lys (experiment 2) or Met (experiment 3).
28918133	15	85	theme	all	2450:2452	arg1	treatments					2466:2475	the all, LH, and LM treatments	2446:2475	the all, LH, and LM treatments	2446:2475	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	4	86	theme	1-d	715:717	arg1	periods					719:725	1-d periods	715:725	1-d periods including 12 h for infusions and 12 h for recovery	715:776	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	4	86	theme	1-d	715:717	arg1	h					740:740	12 h	737:740	12 h for infusions	737:754	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	4	86	theme	1-d	715:717	arg1	h					763:763	12 h	760:763	12 h for recovery	760:776	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	15	87	theme	MUN	2549:2551	arg1	concentration					2553:2565	MUN concentration	2549:2565	MUN concentration	2549:2565	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	9	88	theme	4-14	1426:1429	arg1	PP					1433:1434	4-14 d PP	1426:1434	4-14 d PP	1426:1434	In the fourth experiment, 5 cows (4-14 d PP) were used in a 5 × 5 Latin square design.
28918133	9	88	theme	4-14	1426:1429	arg1	cows					1420:1423	5 cows	1418:1423	5 cows (4-14 d PP)	1418:1435	In the fourth experiment, 5 cows (4-14 d PP) were used in a 5 × 5 Latin square design.
28918133	2	89	theme	dairy	494:498	arg1	diets					504:508	dairy cow diets	494:508	dairy cow diets	494:508	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	8	90	theme	calculated	1303:1312	arg1	requirement					1314:1324	the calculated requirement	1299:1324	the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT)	1299:1389	Treatments were 15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT).
28918133	15	91	located	detected	2405:2412	arg1	experiment					2377:2386	the fourth experiment	2366:2386	the fourth experiment	2366:2386	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	15	91	located	detected	2405:2412	arg2	effects					2392:2398	no effects	2389:2398	no effects	2389:2398	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	6	92	theme	different	1072:1080	arg1	experiment					1123:1132	experiment 2	1123:1134	experiment 2	1123:1134	In the second and third experiments, 8 cows (4-12 d PP) were divided into 2 groups of 4 cows, and each group received a different diet formulated to either be low in Lys (experiment 2) or Met (experiment 3).
28918133	6	92	theme	different	1072:1080	arg1	diet					1082:1085	a different diet	1070:1085	a different diet formulated to either be low in Lys (experiment 2)	1070:1135	In the second and third experiments, 8 cows (4-12 d PP) were divided into 2 groups of 4 cows, and each group received a different diet formulated to either be low in Lys (experiment 2) or Met (experiment 3).
28918133	4	93	theme	Latin	690:694	arg1	design					703:708	a 4 × 4 Latin square design	682:708	a 4 × 4 Latin square design	682:708	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	12	94	theme	milk	2008:2011	arg1	yield					1999:2003	yield	1999:2003	yield	1999:2003	Treatments did not affect DMI or yield of milk or milk components in the first experiment.
28918133	12	94	theme	milk	2008:2011	arg1	DMI					1992:1994	DMI	1992:1994	DMI	1992:1994	Treatments did not affect DMI or yield of milk or milk components in the first experiment.
28918133	0	95	theme	dairy	111:115	arg1	diets					124:128	dairy cattle diets	111:128	dairy cattle diets	111:128	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.
28918133	8	96	theme	Met	1343:1345	arg1	requirement					1314:1324	the calculated requirement	1299:1324	the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT)	1299:1389	Treatments were 15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT).
28918133	1	97	theme	AA	177:178	arg1	addition					156:163	the addition	152:163	the addition of limiting AA	152:178	We hypothesized that the addition of limiting AA increases dry matter intake (DMI) by reducing anaplerosis and hepatic oxidation.
28918133	10	98	dep	MH	1663:1664	arg1	His					1675:1677	His	1675:1677	His	1675:1677	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	10	98	dep	MH	1663:1664	arg1	Met					1667:1669	Met	1667:1669	Met	1667:1669	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	12	99	theme	milk	2016:2019	arg1	components					2021:2030	milk components	2016:2030	milk components	2016:2030	Treatments did not affect DMI or yield of milk or milk components in the first experiment.
28918133	8	100	theme	Lys	1348:1350	arg1	requirement					1314:1324	the calculated requirement	1299:1324	the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT)	1299:1389	Treatments were 15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT).
28918133	1	101	theme	dry	190:192	arg1	DMI					209:211	DMI	209:211	DMI	209:211	We hypothesized that the addition of limiting AA increases dry matter intake (DMI) by reducing anaplerosis and hepatic oxidation.
28918133	1	101	theme	dry	190:192	arg1	intake					201:206	dry matter intake	190:206	dry matter intake (DMI)	190:212	We hypothesized that the addition of limiting AA increases dry matter intake (DMI) by reducing anaplerosis and hepatic oxidation.
28918133	10	102	theme	h.	1559:1560	arg1	Treatments					1562:1571	the first 46 h. Treatments	1546:1571	the first 46 h. Treatments	1546:1571	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	10	103	from	shortage	1717:1724	arg1	AA					1743:1744	each specific AA	1729:1744	each specific AA	1729:1744	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	6	104	theme	second	959:964	arg1	experiments					976:986	the second and third experiments	955:986	the second and third experiments	955:986	In the second and third experiments, 8 cows (4-12 d PP) were divided into 2 groups of 4 cows, and each group received a different diet formulated to either be low in Lys (experiment 2) or Met (experiment 3).
28918133	6	105	from	low	1111:1113	arg1	Lys					1118:1120	Lys	1118:1120	Lys	1118:1120	In the second and third experiments, 8 cows (4-12 d PP) were divided into 2 groups of 4 cows, and each group received a different diet formulated to either be low in Lys (experiment 2) or Met (experiment 3).
28918133	7	106	with	design	1192:1197	arg1	periods					1212:1218	two 1-d periods	1204:1218	two 1-d periods with 12-h infusions (continuous)	1204:1251	Each experiment was a crossover design with two 1-d periods with 12-h infusions (continuous) and 12 h for recovery.
28918133	11	107	theme	milk	1864:1867	arg1	composition					1869:1879	milk composition	1864:1879	milk composition	1864:1879	In each experiment, feed intake was recorded by a computerized data acquisition system, milk yield was recorded, and milk composition was analyzed for fat, protein, lactose, and milk urea nitrogen (MUN) concentrations.
28918133	9	108	theme	square	1464:1469	arg1	design					1471:1476	a 5 × 5 Latin square design	1450:1476	a 5 × 5 Latin square design	1450:1476	In the fourth experiment, 5 cows (4-14 d PP) were used in a 5 × 5 Latin square design.
28918133	18	109	theme	short-term	2990:2999	arg1	infusion					3001:3008	short-term infusion	2990:3008	short-term infusion of AA	2990:3014	Accordingly, short-term infusion of AA is probably not a sensitive method to detect limiting AA in dairy cow diets.
28918133	18	109	theme	short-term	2990:2999	arg1	method					3044:3049	a sensitive method	3032:3049	a sensitive method to detect limiting AA in dairy cow diets	3032:3090	Accordingly, short-term infusion of AA is probably not a sensitive method to detect limiting AA in dairy cow diets.
28918133	13	110	theme	lactose	2137:2143	arg1	percentage					2145:2154	reduced lactose percentage	2129:2154	reduced lactose percentage	2129:2154	In the second experiment, AA treatment increased protein percentage and reduced lactose percentage but had no effect on protein and lactose yields or DMI.
28918133	4	111	theme	first	624:628	arg1	experiment					630:639	The first experiment	620:639	The first experiment	620:639	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	14	112	theme	protein	2302:2308	arg1	yields					2273:2278	yields	2273:2278	yields of milk, lactose, and protein as well as MUN concentration	2273:2337	In the third experiment, the AA treatment tended to increase yields of milk, lactose, and protein as well as MUN concentration but did not affect DMI.
28918133	14	112	theme	protein	2302:2308	arg1	concentration					2325:2337	MUN concentration	2321:2337	yields of milk, lactose, and protein as well as MUN concentration	2273:2337	In the third experiment, the AA treatment tended to increase yields of milk, lactose, and protein as well as MUN concentration but did not affect DMI.
28918133	7	113	with	periods	1212:1218	arg1	infusions					1230:1238	12-h infusions	1225:1238	12-h infusions (continuous)	1225:1251	Each experiment was a crossover design with two 1-d periods with 12-h infusions (continuous) and 12 h for recovery.
28918133	10	114	dep	LH	1641:1642	arg1	His					1653:1655	His	1653:1655	His	1653:1655	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	10	114	dep	LH	1641:1642	arg1	Lys					1645:1647	Lys	1645:1647	Lys	1645:1647	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	7	115	theme	1-d	1208:1210	arg1	periods					1212:1218	two 1-d periods	1204:1218	two 1-d periods with 12-h infusions (continuous)	1204:1251	Each experiment was a crossover design with two 1-d periods with 12-h infusions (continuous) and 12 h for recovery.
28918133	10	116	theme	estimated	1707:1715	arg1	shortage					1717:1724	the estimated shortage	1703:1724	the estimated shortage in each specific AA	1703:1744	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	13	117	theme	protein	2106:2112	arg1	percentage					2114:2123	protein percentage	2106:2123	protein percentage	2106:2123	In the second experiment, AA treatment increased protein percentage and reduced lactose percentage but had no effect on protein and lactose yields or DMI.
28918133	4	118	dep	10	658:659	arg1	to					655:656	to	655:656	to	655:656	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	5	119	theme	calculated	862:871	arg1	requirement					873:883	the calculated requirement	858:883	the calculated requirement of metabolizable Met, Lys, and His	858:918	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	2	120	theme	limiting	416:423	arg1	AA					425:426	the most limiting AA	407:426	the most limiting AA	407:426	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	16	121	theme	limiting	2713:2720	arg1	AA					2722:2723	these potentially limiting AA	2695:2723	these potentially limiting AA	2695:2723	These results failed to provide support for our hypothesis that short-term addition of these potentially limiting AA will increase DMI.
28918133	0	122	theme	limiting	87:94	arg1	acids					102:106	limiting amino acids	87:106	limiting amino acids	87:106	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.
28918133	8	123	theme	%	1375:1375	arg1	CONT					1385:1388	CONT	1385:1388	CONT	1385:1388	Treatments were 15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT).
28918133	8	123	theme	%	1375:1375	arg1	saline					1377:1382	0.9% saline	1372:1382	0.9% saline (CONT)	1372:1389	Treatments were 15% of the calculated requirement of metabolizable Met, Lys, and His (high), or 0.9% saline (CONT).
28918133	7	124	theme	crossover	1182:1190	arg1	experiment					1165:1174	Each experiment	1160:1174	Each experiment	1160:1174	Each experiment was a crossover design with two 1-d periods with 12-h infusions (continuous) and 12 h for recovery.
28918133	7	124	theme	crossover	1182:1190	arg1	design					1192:1197	a crossover design	1180:1197	a crossover design with two 1-d periods with 12-h infusions (continuous)	1180:1251	Each experiment was a crossover design with two 1-d periods with 12-h infusions (continuous) and 12 h for recovery.
28918133	10	125	theme	specific	1734:1741	arg1	AA					1743:1744	each specific AA	1729:1744	each specific AA	1729:1744	Periods were 2 d in which treatments were continuously infused for the first 46 h. Treatments were 0.9% saline (CONT), all (Lys, Met, and His), LM (Lys and Met), LH (Lys and His), and MH (Met and His); dosages were equal to the estimated shortage in each specific AA.
28918133	6	126	theme	cows	1040:1043	arg1	groups					1028:1033	2 groups	1026:1033	2 groups of 4 cows	1026:1043	In the second and third experiments, 8 cows (4-12 d PP) were divided into 2 groups of 4 cows, and each group received a different diet formulated to either be low in Lys (experiment 2) or Met (experiment 3).
28918133	6	126	theme	cows	1040:1043	arg1	cows					1040:1043	4 cows	1038:1043	4 cows	1038:1043	In the second and third experiments, 8 cows (4-12 d PP) were divided into 2 groups of 4 cows, and each group received a different diet formulated to either be low in Lys (experiment 2) or Met (experiment 3).
28918133	5	127	theme	requirement	873:883	arg1	%					846:846	15%	844:846	15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT)	844:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	5	127	theme	requirement	873:883	arg1	saline					928:933	0.9% saline	923:933	0.9% saline (control, CONT)	923:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	5	127	theme	requirement	873:883	arg1	requirement					873:883	the calculated requirement	858:883	the calculated requirement of metabolizable Met, Lys, and His	858:918	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	5	128	theme	5	819:819	arg1	infusions					806:814	continuous infusions	795:814	continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT)	795:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	11	129	theme	data	1810:1813	arg1	system					1827:1832	a computerized data acquisition system	1795:1832	a computerized data acquisition system	1795:1832	In each experiment, feed intake was recorded by a computerized data acquisition system, milk yield was recorded, and milk composition was analyzed for fat, protein, lactose, and milk urea nitrogen (MUN) concentrations.
28918133	4	130	theme	d	661:661	arg1	PP					675:676	PP	675:676	PP	675:676	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	4	130	theme	d	661:661	arg1	postpartum					663:672	d postpartum	661:672	4 cows 6 to 10 d postpartum (PP)	646:677	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	2	131	theme	infusions	354:362	arg1	effects					320:326	the effects	316:326	the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI	316:434	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	2	131	theme	infusions	354:362	arg1	method					441:446	a method	439:446	a method to detect whether specific AA are limiting in dairy cow diets	439:508	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	5	132	theme	metabolizable	888:900	arg1	Met					902:904	metabolizable Met	888:904	metabolizable Met	888:904	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	11	133	theme	milk	1925:1928	arg1	MUN					1945:1947	MUN	1945:1947	MUN	1945:1947	In each experiment, feed intake was recorded by a computerized data acquisition system, milk yield was recorded, and milk composition was analyzed for fat, protein, lactose, and milk urea nitrogen (MUN) concentrations.
28918133	11	133	theme	milk	1925:1928	arg1	nitrogen					1935:1942	milk urea nitrogen	1925:1942	milk urea nitrogen (MUN) concentrations	1925:1963	In each experiment, feed intake was recorded by a computerized data acquisition system, milk yield was recorded, and milk composition was analyzed for fat, protein, lactose, and milk urea nitrogen (MUN) concentrations.
28918133	2	134	theme	short-term	331:340	arg1	infusions					354:362	short-term intravenous infusions	331:362	short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA)	331:427	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	0	135	theme	Short-term	21:30	arg1	infusions					55:63	Short-term intravenous amino acid infusions	21:63	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.	0:129	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.
28918133	5	136	theme	saline	928:933	arg1	%					846:846	15%	844:846	15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT)	844:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	5	136	theme	saline	928:933	arg1	saline					928:933	0.9% saline	923:933	0.9% saline (control, CONT)	923:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	5	136	theme	saline	928:933	arg1	requirement					873:883	the calculated requirement	858:883	the calculated requirement of metabolizable Met, Lys, and His	858:918	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	9	137	used	used	1442:1445	arg2	PP					1433:1434	4-14 d PP	1426:1434	4-14 d PP	1426:1434	In the fourth experiment, 5 cows (4-14 d PP) were used in a 5 × 5 Latin square design.
28918133	9	137	used	used	1442:1445	arg2	cows					1420:1423	5 cows	1418:1423	5 cows (4-14 d PP)	1418:1435	In the fourth experiment, 5 cows (4-14 d PP) were used in a 5 × 5 Latin square design.
28918133	2	138	theme	Lys	372:374	arg1	infusions					354:362	short-term intravenous infusions	331:362	short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA)	331:427	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	0	139	theme	amino	44:48	arg1	infusions					55:63	Short-term intravenous amino acid infusions	21:63	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.	0:129	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.
28918133	15	140	theme	milk	2426:2429	arg1	yield					2431:2435	milk yield	2426:2435	milk yield	2426:2435	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	17	141	theme	His	2884:2886	arg1	effects					2859:2865	the effects	2855:2865	the effects of Lys, Met, and His infusions	2855:2896	This may be due to our hypothesis being inaccurate or to other factors; other limiting AA could have prevented the effects of Lys, Met, and His infusions or the infusion periods could have been too short to induce a response in DMI.
28918133	12	142	theme	first	2039:2043	arg1	experiment					2045:2054	the first experiment	2035:2054	the first experiment	2035:2054	Treatments did not affect DMI or yield of milk or milk components in the first experiment.
28918133	17	143	dep	hypothesis	2767:2776	arg1	inaccurate					2784:2793	inaccurate	2784:2793	inaccurate	2784:2793	This may be due to our hypothesis being inaccurate or to other factors; other limiting AA could have prevented the effects of Lys, Met, and His infusions or the infusion periods could have been too short to induce a response in DMI.
28918133	2	144	theme	Met	367:369	arg1	infusions					354:362	short-term intravenous infusions	331:362	short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA)	331:427	Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets.
28918133	5	145	theme	His	916:918	arg1	saline					928:933	0.9% saline	923:933	0.9% saline (control, CONT)	923:949	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	5	145	theme	His	916:918	arg1	requirement					873:883	the calculated requirement	858:883	the calculated requirement of metabolizable Met, Lys, and His	858:918	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	11	146	theme	nitrogen	1935:1942	arg1	concentrations					1950:1963	milk urea nitrogen (MUN) concentrations	1925:1963	milk urea nitrogen (MUN) concentrations	1925:1963	In each experiment, feed intake was recorded by a computerized data acquisition system, milk yield was recorded, and milk composition was analyzed for fat, protein, lactose, and milk urea nitrogen (MUN) concentrations.
28918133	3	147	theme	postpartum	575:584	arg1	period					586:591	the immediate postpartum period	561:591	the immediate postpartum period	561:591	We conducted 4 experiments using Holstein cows in the immediate postpartum period to address this objective.
28918133	4	148	theme	×	686:686	arg1	design					703:708	a 4 × 4 Latin square design	682:708	a 4 × 4 Latin square design	682:708	The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery.
28918133	11	149	theme	milk	1835:1838	arg1	yield					1840:1844	milk yield	1835:1844	milk yield	1835:1844	In each experiment, feed intake was recorded by a computerized data acquisition system, milk yield was recorded, and milk composition was analyzed for fat, protein, lactose, and milk urea nitrogen (MUN) concentrations.
28918133	5	150	theme	0.9	923:925	arg1	%					926:926	%	926:926	%	926:926	Treatments were continuous infusions of 5 (low), 10 (medium), or 15% (high) of the calculated requirement of metabolizable Met, Lys, and His or 0.9% saline (control, CONT).
28918133	15	151	theme	lactose	2490:2496	arg1	concentration					2498:2510	milk lactose concentration	2485:2510	milk lactose concentration	2485:2510	In the fourth experiment, no effects were detected for DMI and milk yield, whereas the all, LH, and LM treatments reduced milk lactose concentration compared with CONT, and MH increased MUN concentration compared with CONT and other treatments.
28918133	0	152	dep	communication	6:18	arg1	infusions					55:63	Short-term intravenous amino acid infusions	21:63	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.	0:129	Short communication: Short-term intravenous amino acid infusions as a method to detect limiting amino acids in dairy cattle diets.
24621077	5	0	theme	different	584:592	arg1	types					594:598	Three different types	578:598	Three different types of immobilization technique	578:626	Three different types of immobilization technique were carried out.
24621077	2	1	theme	chitosan	355:362	arg1	compositions					339:350	different compositions	329:350	different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA)	329:411	Hydrogels were synthesized via free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA).
24621077	2	2	from	polymerization	311:324	arg1	compositions					339:350	different compositions	329:350	different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA)	329:411	Hydrogels were synthesized via free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA).
24621077	4	3	theme	Cs-P	513:516	arg1	hydrogel					531:538	Cs-P(AAm-co-CA)-2 hydrogel	513:538	Cs-P(AAm-co-CA)-2 hydrogel	513:538	Depending on the swelling results, Cs-P(AAm-co-CA)-2 hydrogel was chosen for lipase immobilization.
24621077	1	4	theme	lipase	244:249	arg1	immobilization					251:264	lipase immobilization	244:264	lipase immobilization	244:264	In this study, a series of semi-interpenetrating polymer network (IPN) hydrogels were prepared as a support material for lipase immobilization.
24621077	6	5	theme	release	653:659	arg1	behaviors					661:669	Lipase release behaviors	646:669	Lipase release behaviors	646:669	Lipase release behaviors were investigated, and immobilization yields of three immobilization methods were compared, and the maximum immobilization yield value was determined for entrapment method.
24621077	4	6	theme	AAm-co-CA	518:526	arg1	hydrogel					531:538	Cs-P(AAm-co-CA)-2 hydrogel	513:538	Cs-P(AAm-co-CA)-2 hydrogel	513:538	Depending on the swelling results, Cs-P(AAm-co-CA)-2 hydrogel was chosen for lipase immobilization.
24621077	1	7	theme	network	180:186	arg1	hydrogels					194:202	semi-interpenetrating polymer network (IPN) hydrogels	150:202	semi-interpenetrating polymer network (IPN) hydrogels	150:202	In this study, a series of semi-interpenetrating polymer network (IPN) hydrogels were prepared as a support material for lipase immobilization.
24621077	1	8	theme	semi-interpenetrating	150:170	arg1	hydrogels					194:202	semi-interpenetrating polymer network (IPN) hydrogels	150:202	semi-interpenetrating polymer network (IPN) hydrogels	150:202	In this study, a series of semi-interpenetrating polymer network (IPN) hydrogels were prepared as a support material for lipase immobilization.
24621077	0	9	theme	citraconic	106:115	arg1	acid					117:120	citraconic acid	106:120	citraconic acid	106:120	Lipase release through semi-interpenetrating polymer network hydrogels based on chitosan, acrylamide, and citraconic acid.
24621077	1	10	theme	polymer	172:178	arg1	hydrogels					194:202	semi-interpenetrating polymer network (IPN) hydrogels	150:202	semi-interpenetrating polymer network (IPN) hydrogels	150:202	In this study, a series of semi-interpenetrating polymer network (IPN) hydrogels were prepared as a support material for lipase immobilization.
24621077	6	11	theme	methods	740:746	arg1	yields					709:714	immobilization yields	694:714	immobilization yields of three immobilization methods	694:746	Lipase release behaviors were investigated, and immobilization yields of three immobilization methods were compared, and the maximum immobilization yield value was determined for entrapment method.
24621077	2	12	theme	acrylamide	370:379	arg1	compositions					339:350	different compositions	329:350	different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA)	329:411	Hydrogels were synthesized via free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA).
24621077	0	13	theme	Lipase	0:5	arg1	release					7:13	Lipase release	0:13	Lipase release through semi-interpenetrating polymer network	0:59	Lipase release through semi-interpenetrating polymer network hydrogels based on chitosan, acrylamide, and citraconic acid.
24621077	4	14	theme	swelling	495:502	arg1	results					504:510	the swelling results	491:510	the swelling results	491:510	Depending on the swelling results, Cs-P(AAm-co-CA)-2 hydrogel was chosen for lipase immobilization.
24621077	6	15	theme	maximum	771:777	arg1	value					800:804	the maximum immobilization yield value	767:804	the maximum immobilization yield value	767:804	Lipase release behaviors were investigated, and immobilization yields of three immobilization methods were compared, and the maximum immobilization yield value was determined for entrapment method.
24621077	6	16	theme	immobilization	725:738	arg1	methods					740:746	three immobilization methods	719:746	three immobilization methods	719:746	Lipase release behaviors were investigated, and immobilization yields of three immobilization methods were compared, and the maximum immobilization yield value was determined for entrapment method.
24621077	2	17	theme	radical	303:309	arg1	polymerization					311:324	free radical polymerization	298:324	free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA)	298:411	Hydrogels were synthesized via free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA).
24621077	0	18	theme	semi-interpenetrating	23:43	arg1	network					53:59	semi-interpenetrating polymer network	23:59	semi-interpenetrating polymer network	23:59	Lipase release through semi-interpenetrating polymer network hydrogels based on chitosan, acrylamide, and citraconic acid.
24621077	2	19	theme	free	298:301	arg1	polymerization					311:324	free radical polymerization	298:324	free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA)	298:411	Hydrogels were synthesized via free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA).
24621077	2	20	theme	citraconic	392:401	arg1	CA					409:410	CA	409:410	CA	409:410	Hydrogels were synthesized via free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA).
24621077	2	20	theme	citraconic	392:401	arg1	acid					403:406	citraconic acid	392:406	citraconic acid (CA)	392:411	Hydrogels were synthesized via free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA).
24621077	2	21	theme	acid	403:406	arg1	compositions					339:350	different compositions	329:350	different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA)	329:411	Hydrogels were synthesized via free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA).
24621077	6	22	theme	entrapment	825:834	arg1	method					836:841	entrapment method	825:841	entrapment method	825:841	Lipase release behaviors were investigated, and immobilization yields of three immobilization methods were compared, and the maximum immobilization yield value was determined for entrapment method.
24621077	6	23	theme	Lipase	646:651	arg1	behaviors					661:669	Lipase release behaviors	646:669	Lipase release behaviors	646:669	Lipase release behaviors were investigated, and immobilization yields of three immobilization methods were compared, and the maximum immobilization yield value was determined for entrapment method.
24621077	1	24	theme	hydrogels	194:202	arg1	series					140:145	a series	138:145	a series of semi-interpenetrating polymer network (IPN) hydrogels	138:202	In this study, a series of semi-interpenetrating polymer network (IPN) hydrogels were prepared as a support material for lipase immobilization.
24621077	1	24	theme	hydrogels	194:202	arg1	material					231:238	a support material	221:238	a support material for lipase immobilization	221:264	In this study, a series of semi-interpenetrating polymer network (IPN) hydrogels were prepared as a support material for lipase immobilization.
24621077	0	25	theme	polymer	45:51	arg1	network					53:59	semi-interpenetrating polymer network	23:59	semi-interpenetrating polymer network	23:59	Lipase release through semi-interpenetrating polymer network hydrogels based on chitosan, acrylamide, and citraconic acid.
24621077	0	26	dep	hydrogels	61:69	arg1	based					71:75	based	71:75	hydrogels based on chitosan, acrylamide, and citraconic acid	61:120	Lipase release through semi-interpenetrating polymer network hydrogels based on chitosan, acrylamide, and citraconic acid.
24621077	5	27	theme	immobilization	603:616	arg1	technique					618:626	immobilization technique	603:626	immobilization technique	603:626	Three different types of immobilization technique were carried out.
24621077	3	28	theme	hydrogels	441:449	arg1	%					475:475	240-400%	468:475	240-400%	468:475	The swelling values of the hydrogels were found to be 240-400%.
24621077	3	28	theme	hydrogels	441:449	arg1	values					427:432	The swelling values	414:432	The swelling values of the hydrogels	414:449	The swelling values of the hydrogels were found to be 240-400%.
24621077	5	29	theme	technique	618:626	arg1	types					594:598	Three different types	578:598	Three different types of immobilization technique	578:626	Three different types of immobilization technique were carried out.
24621077	6	30	theme	immobilization	694:707	arg1	yields					709:714	immobilization yields	694:714	immobilization yields of three immobilization methods	694:746	Lipase release behaviors were investigated, and immobilization yields of three immobilization methods were compared, and the maximum immobilization yield value was determined for entrapment method.
24621077	6	31	theme	yield	794:798	arg1	value					800:804	the maximum immobilization yield value	767:804	the maximum immobilization yield value	767:804	Lipase release behaviors were investigated, and immobilization yields of three immobilization methods were compared, and the maximum immobilization yield value was determined for entrapment method.
24621077	2	32	theme	different	329:337	arg1	compositions					339:350	different compositions	329:350	different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA)	329:411	Hydrogels were synthesized via free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA).
24621077	6	33	theme	immobilization	779:792	arg1	value					800:804	the maximum immobilization yield value	767:804	the maximum immobilization yield value	767:804	Lipase release behaviors were investigated, and immobilization yields of three immobilization methods were compared, and the maximum immobilization yield value was determined for entrapment method.
24621077	1	34	theme	IPN	189:191	arg1	hydrogels					194:202	semi-interpenetrating polymer network (IPN) hydrogels	150:202	semi-interpenetrating polymer network (IPN) hydrogels	150:202	In this study, a series of semi-interpenetrating polymer network (IPN) hydrogels were prepared as a support material for lipase immobilization.
24621077	3	35	theme	swelling	418:425	arg1	%					475:475	240-400%	468:475	240-400%	468:475	The swelling values of the hydrogels were found to be 240-400%.
24621077	3	35	theme	swelling	418:425	arg1	values					427:432	The swelling values	414:432	The swelling values of the hydrogels	414:449	The swelling values of the hydrogels were found to be 240-400%.
24621077	4	36	theme	lipase	555:560	arg1	immobilization					562:575	lipase immobilization	555:575	lipase immobilization	555:575	Depending on the swelling results, Cs-P(AAm-co-CA)-2 hydrogel was chosen for lipase immobilization.
24621077	1	37	theme	support	223:229	arg1	series					140:145	a series	138:145	a series of semi-interpenetrating polymer network (IPN) hydrogels	138:202	In this study, a series of semi-interpenetrating polymer network (IPN) hydrogels were prepared as a support material for lipase immobilization.
24621077	1	37	theme	support	223:229	arg1	material					231:238	a support material	221:238	a support material for lipase immobilization	221:264	In this study, a series of semi-interpenetrating polymer network (IPN) hydrogels were prepared as a support material for lipase immobilization.
28926673	0	0	theme	CHO	105:107	arg1	cells					109:113	CHO cells	105:113	CHO cells	105:113	Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cells.
28926673	7	1	theme	production	1206:1215	arg1	platform					1217:1224	a new antibody production platform	1191:1224	a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications	1191:1342	Combining genome and protein engineering in CHO cells will provide a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications.
28926673	1	2	theme	antibodies	164:173	arg1	attributes					139:148	the key quality attributes	123:148	the key quality attributes of monoclonal antibodies	123:173	One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution.
28926673	2	3	from	antibodies	310:319	arg1	glycans					297:303	the total glycans	287:303	the total glycans from antibodies	287:319	Two terminal galactose residues typically represent a small fraction of the total glycans from antibodies.
28926673	2	3	from	antibodies	310:319	arg1	fraction					275:282	a small fraction	267:282	a small fraction of the total glycans from antibodies	267:319	Two terminal galactose residues typically represent a small fraction of the total glycans from antibodies.
28926673	4	4	theme	CHO	616:618	arg1	cells					621:625	Chinese Hamster Ovary (CHO) cells	593:625	Chinese Hamster Ovary (CHO) cells	593:625	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	5	5	theme	same	785:788	arg1	IgG					823:825	the same single amino acid mutant (F241A) IgG	781:825	the same single amino acid mutant (F241A) IgG	781:825	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	6	6	theme	amino	1041:1045	arg1	substitutions					1052:1064	four amino acid substitutions	1036:1064	four amino acid substitutions	1036:1064	However, overexpression of IgGs with four amino acid substitutions recovered the G2 glycoform composition approximately 80%.
28926673	5	7	from	Expression	767:776	arg1	cells					834:838	CHO cells	830:838	CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6	830:920	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	6	8	theme	G2	1080:1081	arg1	composition					1093:1103	the G2 glycoform composition	1076:1103	the G2 glycoform composition approximately 80%	1076:1121	However, overexpression of IgGs with four amino acid substitutions recovered the G2 glycoform composition approximately 80%.
28926673	6	9	theme	acid	1047:1050	arg1	substitutions					1052:1064	four amino acid substitutions	1036:1064	four amino acid substitutions	1036:1064	However, overexpression of IgGs with four amino acid substitutions recovered the G2 glycoform composition approximately 80%.
28926673	3	10	theme	enhanced	390:397	arg1	galactosylation					399:413	enhanced galactosylation	390:413	enhanced galactosylation	390:413	However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities.
28926673	4	11	contain	containing	701:710	arg1	IgG					697:699	an IgG	694:699	an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms	694:764	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	11	contain	containing	701:710	arg2	%					714:714	80% bigalactosylated	712:731	80% bigalactosylated	712:731	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	11	contain	containing	701:710	arg2	glycoforms					755:764	fucosylated (G2F) glycoforms	737:764	fucosylated (G2F) glycoforms	737:764	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	5	12	theme	fucosyltransferase	871:888	arg1	knockout					859:866	knockout	859:866	knockout	859:866	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	7	13	theme	antibody	1197:1204	arg1	platform					1217:1224	a new antibody production platform	1191:1224	a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications	1191:1342	Combining genome and protein engineering in CHO cells will provide a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications.
28926673	7	14	theme	protein	1145:1151	arg1	engineering					1153:1163	protein engineering	1145:1163	protein engineering	1145:1163	Combining genome and protein engineering in CHO cells will provide a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications.
28926673	2	15	theme	glycans	297:303	arg1	fraction					275:282	a small fraction	267:282	a small fraction of the total glycans from antibodies	267:319	Two terminal galactose residues typically represent a small fraction of the total glycans from antibodies.
28926673	4	16	theme	fucosylated	737:747	arg1	glycoforms					755:764	fucosylated (G2F) glycoforms	737:764	fucosylated (G2F) glycoforms	737:764	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	17	gly	glycoforms	755:764	arg1	G2F					750:752	fucosylated (G2F) glycoforms	737:764	fucosylated (G2F) glycoforms	737:764	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	7	18	theme	specific	1293:1300	arg1	applications					1331:1342	specific biomedical and biotechnology applications	1293:1342	specific biomedical and biotechnology applications	1293:1342	Combining genome and protein engineering in CHO cells will provide a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications.
28926673	6	19	theme	glycoform	1083:1091	arg1	composition					1093:1103	the G2 glycoform composition	1076:1103	the G2 glycoform composition approximately 80%	1076:1121	However, overexpression of IgGs with four amino acid substitutions recovered the G2 glycoform composition approximately 80%.
28926673	5	20	theme	triple	847:852	arg1	gene					854:857	a triple gene	845:857	a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6	845:920	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	2	21	theme	total	291:295	arg1	glycans					297:303	the total glycans	287:303	the total glycans from antibodies	287:319	Two terminal galactose residues typically represent a small fraction of the total glycans from antibodies.
28926673	3	22	theme	biomedical	481:490	arg1	modalities					492:501	these important biomedical modalities	465:501	these important biomedical modalities	465:501	However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities.
28926673	0	23	theme	monoclonal	52:61	arg1	antibodies					63:72	monoclonal antibodies	52:72	monoclonal antibodies	52:72	Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cells.
28926673	4	24	from	disruption	523:532	arg1	cells					621:625	Chinese Hamster Ovary (CHO) cells	593:625	Chinese Hamster Ovary (CHO) cells	593:625	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	7	25	theme	new	1193:1195	arg1	antibody					1197:1204	new antibody	1193:1204	a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications	1191:1342	Combining genome and protein engineering in CHO cells will provide a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications.
28926673	5	26	theme	F241A	816:820	arg1	IgG					823:825	the same single amino acid mutant (F241A) IgG	781:825	the same single amino acid mutant (F241A) IgG	781:825	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	1	27	theme	glycan	182:187	arg1	pattern					189:195	the glycan pattern	178:195	the glycan pattern	178:195	One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution.
28926673	1	27	theme	glycan	182:187	arg1	attributes					139:148	the key quality attributes	123:148	the key quality attributes of monoclonal antibodies	123:173	One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution.
28926673	1	27	theme	glycan	182:187	arg1	One					116:118	One	116:118	One	116:118	One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution.
28926673	6	28	dep	composition	1093:1103	arg1	%					1121:1121	%	1121:1121	%	1121:1121	However, overexpression of IgGs with four amino acid substitutions recovered the G2 glycoform composition approximately 80%.
28926673	0	29	theme	Combinatorial	0:12	arg1	genome					14:19	Combinatorial genome	0:19	Combinatorial genome	0:19	Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cells.
28926673	4	30	theme	Chinese	593:599	arg1	cells					621:625	Chinese Hamster Ovary (CHO) cells	593:625	Chinese Hamster Ovary (CHO) cells	593:625	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	31	theme	G2F	750:752	arg1	glycoforms					755:764	fucosylated (G2F) glycoforms	737:764	fucosylated (G2F) glycoforms	737:764	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	7	32	theme	biotechnology	1317:1329	arg1	applications					1331:1342	specific biomedical and biotechnology applications	1293:1342	specific biomedical and biotechnology applications	1293:1342	Combining genome and protein engineering in CHO cells will provide a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications.
28926673	3	33	theme	important	471:479	arg1	modalities					492:501	these important biomedical modalities	465:501	these important biomedical modalities	465:501	However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities.
28926673	0	34	theme	protein	25:31	arg1	engineering					33:43	protein engineering	25:43	protein engineering	25:43	Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cells.
28926673	4	35	dep	sialyltransferases	547:564	arg1	ST3GAL6					579:585	ST3GAL6	579:585	ST3GAL6	579:585	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	35	dep	sialyltransferases	547:564	arg1	ST3GAL4					567:573	ST3GAL4	567:573	ST3GAL4	567:573	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	35	dep	sialyltransferases	547:564	arg1	sialyltransferases					547:564	two α-2,3 sialyltransferases	537:564	two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells	537:625	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	6	36	with	IgGs	1026:1029	arg1	substitutions					1052:1064	four amino acid substitutions	1036:1064	four amino acid substitutions	1036:1064	However, overexpression of IgGs with four amino acid substitutions recovered the G2 glycoform composition approximately 80%.
28926673	5	37	dep	glycans	990:996	arg1	%					968:968	%	968:968	%	968:968	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	5	38	theme	IgG	823:825	arg1	Expression					767:776	Expression	767:776	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6	767:920	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	4	39	theme	Ovary	609:613	arg1	cells					621:625	Chinese Hamster Ovary (CHO) cells	593:625	Chinese Hamster Ovary (CHO) cells	593:625	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	6	40	dep	%	1121:1121	arg1	80					1119:1120	80	1119:1120	80	1119:1120	However, overexpression of IgGs with four amino acid substitutions recovered the G2 glycoform composition approximately 80%.
28926673	5	41	theme	CHO	830:832	arg1	cells					834:838	CHO cells	830:838	CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6	830:920	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	5	42	theme	single	790:795	arg1	IgG					823:825	the same single amino acid mutant (F241A) IgG	781:825	the same single amino acid mutant (F241A) IgG	781:825	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	4	43	theme	Hamster	601:607	arg1	cells					621:625	Chinese Hamster Ovary (CHO) cells	593:625	Chinese Hamster Ovary (CHO) cells	593:625	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	44	gly	fucosylated	737:747	arg1	glycoforms					755:764	fucosylated (G2F) glycoforms	737:764	fucosylated (G2F) glycoforms	737:764	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	2	45	from	fraction	275:282	arg1	antibodies					310:319	antibodies	310:319	antibodies	310:319	Two terminal galactose residues typically represent a small fraction of the total glycans from antibodies.
28926673	4	46	theme	Fc	672:673	arg1	region					675:680	the Fc region	668:680	the Fc region	668:680	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	5	47	theme	amino	797:801	arg1	IgG					823:825	the same single amino acid mutant (F241A) IgG	781:825	the same single amino acid mutant (F241A) IgG	781:825	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	6	48	theme	IgGs	1026:1029	arg1	overexpression					1008:1021	overexpression	1008:1021	overexpression of IgGs with four amino acid substitutions	1008:1064	However, overexpression of IgGs with four amino acid substitutions recovered the G2 glycoform composition approximately 80%.
28926673	5	49	theme	acid	803:806	arg1	IgG					823:825	the same single amino acid mutant (F241A) IgG	781:825	the same single amino acid mutant (F241A) IgG	781:825	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	4	50	from	cells	621:625	arg1	disruption					523:532	the disruption	519:532	the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells	519:625	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	50	from	cells	621:625	arg1	ST3GAL6					579:585	ST3GAL6	579:585	ST3GAL6	579:585	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	50	from	cells	621:625	arg1	ST3GAL4					567:573	ST3GAL4	567:573	ST3GAL4	567:573	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	50	from	cells	621:625	arg1	sialyltransferases					547:564	two α-2,3 sialyltransferases	537:564	two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells	537:625	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	5	51	theme	mutant	808:813	arg1	IgG					823:825	the same single amino acid mutant (F241A) IgG	781:825	the same single amino acid mutant (F241A) IgG	781:825	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	5	52	dep	65	966:967	arg1	to					963:964	to	963:964	to	963:964	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	3	53	theme	altered	442:448	arg1	properties					450:459	altered properties	442:459	altered properties for these important biomedical modalities	442:501	However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities.
28926673	5	54	with	cells	834:838	arg1	gene					854:857	a triple gene	845:857	a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6	845:920	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	2	55	theme	terminal	219:226	arg1	residues					238:245	Two terminal galactose residues	215:245	Two terminal galactose residues	215:245	Two terminal galactose residues typically represent a small fraction of the total glycans from antibodies.
28926673	2	56	theme	galactose	228:236	arg1	residues					238:245	Two terminal galactose residues	215:245	Two terminal galactose residues	215:245	Two terminal galactose residues typically represent a small fraction of the total glycans from antibodies.
28926673	1	57	theme	key	127:129	arg1	attributes					139:148	the key quality attributes	123:148	the key quality attributes of monoclonal antibodies	123:173	One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution.
28926673	7	58	theme	glycoforms	1268:1277	arg1	standards					1279:1287	glycoforms standards	1268:1287	glycoforms standards for specific biomedical and biotechnology applications	1268:1342	Combining genome and protein engineering in CHO cells will provide a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications.
28926673	5	59	theme	bigalactosylated	970:985	arg1	glycans					990:996	the galactosylation glycoprofile to 65% bigalactosylated G2 glycans	930:996	the galactosylation glycoprofile to 65% bigalactosylated G2 glycans	930:996	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	3	60	with	antibodies	331:340	arg1	properties					369:378	defined glycosylation properties	347:378	defined glycosylation properties including enhanced galactosylation	347:413	However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities.
28926673	3	60	with	antibodies	331:340	arg1	galactosylation					399:413	enhanced galactosylation	390:413	enhanced galactosylation	390:413	However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities.
28926673	1	61	theme	quality	131:137	arg1	attributes					139:148	the key quality attributes	123:148	the key quality attributes of monoclonal antibodies	123:173	One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution.
28926673	5	62	theme	knockout	859:866	arg1	gene					854:857	a triple gene	845:857	a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6	845:920	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	4	63	theme	sialyltransferases	547:564	arg1	disruption					523:532	the disruption	519:532	the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells	519:625	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	5	64	theme	G2	987:988	arg1	glycans					990:996	the galactosylation glycoprofile to 65% bigalactosylated G2 glycans	930:996	the galactosylation glycoprofile to 65% bigalactosylated G2 glycans	930:996	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	1	65	theme	attributes	139:148	arg1	pattern					189:195	the glycan pattern	178:195	the glycan pattern	178:195	One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution.
28926673	1	65	theme	attributes	139:148	arg1	attributes					139:148	the key quality attributes	123:148	the key quality attributes of monoclonal antibodies	123:173	One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution.
28926673	1	65	theme	attributes	139:148	arg1	One					116:118	One	116:118	One	116:118	One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution.
28926673	5	66	theme	ST3GAL4	902:908	arg1	knockout					859:866	knockout	859:866	knockout	859:866	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	3	67	theme	defined	347:353	arg1	properties					369:378	defined glycosylation properties	347:378	defined glycosylation properties including enhanced galactosylation	347:413	However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities.
28926673	3	67	theme	defined	347:353	arg1	galactosylation					399:413	enhanced galactosylation	390:413	enhanced galactosylation	390:413	However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities.
28926673	0	68	from	cells	109:113	arg1	hypergalactosylation					79:98	hypergalactosylation	79:98	hypergalactosylation from CHO cells	79:113	Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cells.
28926673	2	69	theme	small	269:273	arg1	fraction					275:282	a small fraction	267:282	a small fraction of the total glycans from antibodies	267:319	Two terminal galactose residues typically represent a small fraction of the total glycans from antibodies.
28926673	5	70	dep	%	968:968	arg1	65					966:967	65	966:967	65	966:967	Expression of the same single amino acid mutant (F241A) IgG in CHO cells with a triple gene knockout of fucosyltransferase (FUT8) plus ST3GAL4 and ST3GAL6 lowered the galactosylation glycoprofile to 65% bigalactosylated G2 glycans.
28926673	4	71	theme	region	675:680	arg1	engineering					653:663	protein engineering	645:663	protein engineering of the Fc region	645:680	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	72	theme	protein	645:651	arg1	engineering					653:663	protein engineering	645:663	protein engineering of the Fc region	645:680	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	3	73	theme	glycosylation	355:367	arg1	properties					369:378	defined glycosylation properties	347:378	defined glycosylation properties including enhanced galactosylation	347:413	However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities.
28926673	3	73	theme	glycosylation	355:367	arg1	galactosylation					399:413	enhanced galactosylation	390:413	enhanced galactosylation	390:413	However, antibodies with defined glycosylation properties including enhanced galactosylation have been shown to exhibit altered properties for these important biomedical modalities.
28926673	4	74	theme	α-2,3	541:545	arg1	ST3GAL6					579:585	ST3GAL6	579:585	ST3GAL6	579:585	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	74	theme	α-2,3	541:545	arg1	ST3GAL4					567:573	ST3GAL4	567:573	ST3GAL4	567:573	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	4	74	theme	α-2,3	541:545	arg1	sialyltransferases					547:564	two α-2,3 sialyltransferases	537:564	two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells	537:625	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
28926673	7	75	theme	CHO	1168:1170	arg1	cells					1172:1176	CHO cells	1168:1176	CHO cells	1168:1176	Combining genome and protein engineering in CHO cells will provide a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications.
28926673	1	76	theme	monoclonal	153:162	arg1	antibodies					164:173	monoclonal antibodies	153:173	monoclonal antibodies	153:173	One of the key quality attributes of monoclonal antibodies is the glycan pattern and distribution.
28926673	7	77	theme	biomedical	1302:1311	arg1	applications					1331:1342	specific biomedical and biotechnology applications	1293:1342	specific biomedical and biotechnology applications	1293:1342	Combining genome and protein engineering in CHO cells will provide a new antibody production platform that enables biotechnologists to generate glycoforms standards for specific biomedical and biotechnology applications.
28926673	4	78	theme	bigalactosylated	716:731	arg1	%					714:714	80% bigalactosylated	712:731	80% bigalactosylated	712:731	In this study, the disruption of two α-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% bigalactosylated and fucosylated (G2F) glycoforms.
29310303	1	0	theme	black	352:356	arg1	Au--In2O3--CS/ABPE					375:392	Au--In2O3--CS/ABPE	375:392	Au--In2O3--CS/ABPE	375:392	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	1	0	theme	black	352:356	arg1	electrode					364:372	acetylene black paste electrode	342:372	acetylene black paste electrode (Au--In2O3--CS/ABPE)	342:393	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	1	1	theme	first	169:173	arg1	time					175:178	the first time	165:178	the first time	165:178	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	6	2	from	detection	1249:1257	arg1	formulations					1284:1295	pharmaceutical formulations	1269:1295	pharmaceutical formulations with good accuracy and precision	1269:1328	The analytical performance of this biosensor was evaluated for detection of CPX in pharmaceutical formulations with good accuracy and precision.
29310303	0	3	theme	paste	86:90	arg1	electrode					92:100	acetylene black paste electrode	70:100	acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine	70:158	A novel sensor based on nanobiocomposite Au--In2O3--chitosan modified acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine.
29310303	1	4	theme	paste	358:362	arg1	Au--In2O3--CS/ABPE					375:392	Au--In2O3--CS/ABPE	375:392	Au--In2O3--CS/ABPE	375:392	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	1	4	theme	paste	358:362	arg1	electrode					364:372	acetylene black paste electrode	342:372	acetylene black paste electrode (Au--In2O3--CS/ABPE)	342:393	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	4	5	theme	excellent	826:834	arg1	activity					852:859	an excellent electrochemical activity	823:859	an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE	823:1013	The modified electrode showed an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE.
29310303	0	6	theme	sensitive	106:114	arg1	detection					116:124	sensitive detection	106:124	sensitive detection of antimycotic ciclopirox olamine	106:158	A novel sensor based on nanobiocomposite Au--In2O3--chitosan modified acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine.
29310303	1	7	theme	electrode	364:372	arg1	modification					326:337	the modification	322:337	the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE)	322:393	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	5	8	theme	10-9molL-1	1170:1179	arg1	CPX					1181:1183	6.64 × 10-9molL-1 CPX	1163:1183	6.64 × 10-9molL-1 CPX	1163:1183	The proposed biosensor demonstrated linearity in the range 0.199 - 16.22µmolL-1, with high sensitivity (64.57μAµmolL-1cm-2) and detection limit of 6.64 × 10-9molL-1 CPX.
29310303	7	9	theme	%	1416:1416	arg1	level					1429:1433	the 95% confidence level	1410:1433	the 95% confidence level	1410:1433	This proposed method was validated by UPLC and the results are in agreement at the 95% confidence level.
29310303	1	10	used	used	313:316	arg2	sensor					221:226	a sensitive conductive nanobiocomposite sensor	181:226	a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS)	181:281	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	5	11	theme	proposed	1020:1027	arg1	biosensor					1029:1037	The proposed biosensor	1016:1037	The proposed biosensor	1016:1037	The proposed biosensor demonstrated linearity in the range 0.199 - 16.22µmolL-1, with high sensitivity (64.57μAµmolL-1cm-2) and detection limit of 6.64 × 10-9molL-1 CPX.
29310303	6	12	theme	CPX	1262:1264	arg1	detection					1249:1257	detection	1249:1257	detection of CPX in pharmaceutical formulations with good accuracy and precision	1249:1328	The analytical performance of this biosensor was evaluated for detection of CPX in pharmaceutical formulations with good accuracy and precision.
29310303	4	13	theme	modified	797:804	arg1	electrode					806:814	The modified electrode	793:814	The modified electrode	793:814	The modified electrode showed an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE.
29310303	3	14	theme	square	768:773	arg1	wave					775:778	square wave	768:778	square wave voltammetry	768:790	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	2	15	theme	electron	564:571	arg1	TEM					585:587	TEM	585:587	TEM	585:587	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	2	15	theme	electron	564:571	arg1	microscopy					573:582	transmission electron microscopy	551:582	transmission electron microscopy (TEM)	551:588	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	2	16	theme	phase	400:404	arg1	structures					406:415	The phase structures	396:415	The phase structures	396:415	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	2	16	theme	phase	400:404	arg1	morphology					434:443	morphology	434:443	morphology	434:443	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	2	16	theme	phase	400:404	arg1	composition					418:428	composition	418:428	composition	418:428	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	5	17	theme	detection	1144:1152	arg1	limit					1154:1158	detection limit	1144:1158	detection limit of 6.64 × 10-9molL-1 CPX	1144:1183	The proposed biosensor demonstrated linearity in the range 0.199 - 16.22µmolL-1, with high sensitivity (64.57μAµmolL-1cm-2) and detection limit of 6.64 × 10-9molL-1 CPX.
29310303	3	18	theme	cyclic	745:750	arg1	voltammetry					752:762	cyclic voltammetry	745:762	cyclic voltammetry	745:762	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	4	19	theme	antimycotic	897:907	arg1	olamine					920:926	the antimycotic ciclopirox olamine	893:926	the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE	893:1013	The modified electrode showed an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE.
29310303	4	19	theme	antimycotic	897:907	arg1	CPX					929:931	CPX	929:931	CPX	929:931	The modified electrode showed an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE.
29310303	6	20	theme	biosensor	1221:1229	arg1	performance					1201:1211	The analytical performance	1186:1211	The analytical performance of this biosensor	1186:1229	The analytical performance of this biosensor was evaluated for detection of CPX in pharmaceutical formulations with good accuracy and precision.
29310303	5	21	from	linearity	1052:1060	arg1	range					1069:1073	the range 0.199	1065:1079	the range 0.199	1065:1079	The proposed biosensor demonstrated linearity in the range 0.199 - 16.22µmolL-1, with high sensitivity (64.57μAµmolL-1cm-2) and detection limit of 6.64 × 10-9molL-1 CPX.
29310303	0	22	theme	novel	2:6	arg1	sensor					8:13	A novel sensor	0:13	A novel sensor based on nanobiocomposite Au--In2O3--chitosan	0:59	A novel sensor based on nanobiocomposite Au--In2O3--chitosan modified acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine.
29310303	5	23	theme	×	1168:1168	arg1	CPX					1181:1183	6.64 × 10-9molL-1 CPX	1163:1183	6.64 × 10-9molL-1 CPX	1163:1183	The proposed biosensor demonstrated linearity in the range 0.199 - 16.22µmolL-1, with high sensitivity (64.57μAµmolL-1cm-2) and detection limit of 6.64 × 10-9molL-1 CPX.
29310303	2	24	theme	energy-dispersed	518:533	arg1	EDX					542:544	EDX	542:544	EDX	542:544	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	2	24	theme	energy-dispersed	518:533	arg1	X-ray					535:539	energy-dispersed X-ray	518:539	energy-dispersed X-ray (EDX)	518:545	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	1	25	theme	sensitive	183:191	arg1	sensor					221:226	a sensitive conductive nanobiocomposite sensor	181:226	a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS)	181:281	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	4	26	theme	olamine	920:926	arg1	electro-oxidation					872:888	the electro-oxidation	868:888	the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE	868:1013	The modified electrode showed an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE.
29310303	4	27	theme	ciclopirox	909:918	arg1	olamine					920:926	the antimycotic ciclopirox olamine	893:926	the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE	893:1013	The modified electrode showed an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE.
29310303	4	27	theme	ciclopirox	909:918	arg1	CPX					929:931	CPX	929:931	CPX	929:931	The modified electrode showed an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE.
29310303	1	28	theme	conductive	193:202	arg1	sensor					221:226	a sensitive conductive nanobiocomposite sensor	181:226	a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS)	181:281	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	2	29	theme	nanocomposite	457:469	arg1	structures					406:415	The phase structures	396:415	The phase structures	396:415	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	2	29	theme	nanocomposite	457:469	arg1	morphology					434:443	morphology	434:443	morphology	434:443	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	2	29	theme	nanocomposite	457:469	arg1	composition					418:428	composition	418:428	composition	418:428	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	0	30	theme	ciclopirox	141:150	arg1	olamine					152:158	antimycotic ciclopirox olamine	129:158	antimycotic ciclopirox olamine	129:158	A novel sensor based on nanobiocomposite Au--In2O3--chitosan modified acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine.
29310303	6	31	theme	pharmaceutical	1269:1282	arg1	formulations					1284:1295	pharmaceutical formulations	1269:1295	pharmaceutical formulations with good accuracy and precision	1269:1328	The analytical performance of this biosensor was evaluated for detection of CPX in pharmaceutical formulations with good accuracy and precision.
29310303	1	32	theme	nanobiocomposite	204:219	arg1	sensor					221:226	a sensitive conductive nanobiocomposite sensor	181:226	a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS)	181:281	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	2	33	theme	Au-In2O3	448:455	arg1	nanocomposite					457:469	Au-In2O3 nanocomposite	448:469	Au-In2O3 nanocomposite	448:469	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	5	34	theme	CPX	1181:1183	arg1	sensitivity					1107:1117	high sensitivity	1102:1117	high sensitivity (64.57μAµmolL-1cm-2)	1102:1138	The proposed biosensor demonstrated linearity in the range 0.199 - 16.22µmolL-1, with high sensitivity (64.57μAµmolL-1cm-2) and detection limit of 6.64 × 10-9molL-1 CPX.
29310303	5	34	theme	CPX	1181:1183	arg1	limit					1154:1158	detection limit	1144:1158	detection limit of 6.64 × 10-9molL-1 CPX	1144:1183	The proposed biosensor demonstrated linearity in the range 0.199 - 16.22µmolL-1, with high sensitivity (64.57μAµmolL-1cm-2) and detection limit of 6.64 × 10-9molL-1 CPX.
29310303	5	34	theme	CPX	1181:1183	arg1	64.57μAµmolL-1cm-2					1120:1137	64.57μAµmolL-1cm-2	1120:1137	64.57μAµmolL-1cm-2	1120:1137	The proposed biosensor demonstrated linearity in the range 0.199 - 16.22µmolL-1, with high sensitivity (64.57μAµmolL-1cm-2) and detection limit of 6.64 × 10-9molL-1 CPX.
29310303	0	35	theme	antimycotic	129:139	arg1	olamine					152:158	antimycotic ciclopirox olamine	129:158	antimycotic ciclopirox olamine	129:158	A novel sensor based on nanobiocomposite Au--In2O3--chitosan modified acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine.
29310303	6	36	theme	analytical	1190:1199	arg1	performance					1201:1211	The analytical performance	1186:1211	The analytical performance of this biosensor	1186:1229	The analytical performance of this biosensor was evaluated for detection of CPX in pharmaceutical formulations with good accuracy and precision.
29310303	7	37	from	level	1429:1433	arg1	agreement					1397:1405	agreement	1397:1405	agreement	1397:1405	This proposed method was validated by UPLC and the results are in agreement at the 95% confidence level.
29310303	7	38	theme	95	1414:1415	arg1	%					1416:1416	%	1416:1416	%	1416:1416	This proposed method was validated by UPLC and the results are in agreement at the 95% confidence level.
29310303	0	39	theme	nanobiocomposite	24:39	arg1	Au--In2O3--chitosan					41:59	nanobiocomposite Au--In2O3--chitosan	24:59	nanobiocomposite Au--In2O3--chitosan	24:59	A novel sensor based on nanobiocomposite Au--In2O3--chitosan modified acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine.
29310303	2	40	theme	transmission	551:562	arg1	TEM					585:587	TEM	585:587	TEM	585:587	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	2	40	theme	transmission	551:562	arg1	microscopy					573:582	transmission electron microscopy	551:582	transmission electron microscopy (TEM)	551:588	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	6	41	theme	good	1302:1305	arg1	accuracy					1307:1314	good accuracy	1302:1314	good accuracy	1302:1314	The analytical performance of this biosensor was evaluated for detection of CPX in pharmaceutical formulations with good accuracy and precision.
29310303	0	42	theme	olamine	152:158	arg1	detection					116:124	sensitive detection	106:124	sensitive detection of antimycotic ciclopirox olamine	106:158	A novel sensor based on nanobiocomposite Au--In2O3--chitosan modified acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine.
29310303	3	43	theme	electrode	656:664	arg1	Au--In2O3--CS/ABPE					666:683	the biosensor electrode Au--In2O3--CS/ABPE	642:683	the biosensor electrode Au--In2O3--CS/ABPE	642:683	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	3	44	theme	surface	622:628	arg1	analysis					630:637	surface analysis	622:637	surface analysis of the biosensor electrode Au--In2O3--CS/ABPE	622:683	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	7	45	theme	confidence	1418:1427	arg1	level					1429:1433	the 95% confidence level	1410:1433	the 95% confidence level	1410:1433	This proposed method was validated by UPLC and the results are in agreement at the 95% confidence level.
29310303	2	46	theme	X-ray	493:497	arg1	XRD					512:514	XRD	512:514	XRD	512:514	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	2	46	theme	X-ray	493:497	arg1	diffraction					499:509	X-ray diffraction	493:509	X-ray diffraction (XRD)	493:515	The phase structures, composition and morphology of Au-In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy-dispersed X-ray (EDX) and transmission electron microscopy (TEM).
29310303	4	47	theme	bare	1005:1008	arg1	ABPE					1010:1013	the bare ABPE	1001:1013	the bare ABPE	1001:1013	The modified electrode showed an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE.
29310303	4	48	theme	significant	947:957	arg1	improvement					959:969	a significant improvement	945:969	a significant improvement in sensitivity	945:984	The modified electrode showed an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE.
29310303	3	49	theme	Au--In2O3--CS/ABPE	666:683	arg1	activities					607:616	Electrochemical activities	591:616	Electrochemical activities	591:616	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	3	49	theme	Au--In2O3--CS/ABPE	666:683	arg1	analysis					630:637	surface analysis	622:637	surface analysis of the biosensor electrode Au--In2O3--CS/ABPE	622:683	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	3	50	theme	Electrochemical	591:605	arg1	activities					607:616	Electrochemical activities	591:616	Electrochemical activities	591:616	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	5	51	theme	6.64	1163:1166	arg1	CPX					1181:1183	6.64 × 10-9molL-1 CPX	1163:1183	6.64 × 10-9molL-1 CPX	1163:1183	The proposed biosensor demonstrated linearity in the range 0.199 - 16.22µmolL-1, with high sensitivity (64.57μAµmolL-1cm-2) and detection limit of 6.64 × 10-9molL-1 CPX.
29310303	3	52	dep	wave	775:778	arg1	voltammetry					780:790	voltammetry	780:790	square wave voltammetry	768:790	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	3	53	theme	electron	718:725	arg1	SEM					739:741	SEM	739:741	SEM	739:741	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	3	53	theme	electron	718:725	arg1	microscopy					727:736	scanning electron microscopy	709:736	scanning electron microscopy (SEM)	709:742	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	5	54	theme	high	1102:1105	arg1	sensitivity					1107:1117	high sensitivity	1102:1117	high sensitivity (64.57μAµmolL-1cm-2)	1102:1138	The proposed biosensor demonstrated linearity in the range 0.199 - 16.22µmolL-1, with high sensitivity (64.57μAµmolL-1cm-2) and detection limit of 6.64 × 10-9molL-1 CPX.
29310303	5	54	theme	high	1102:1105	arg1	64.57μAµmolL-1cm-2					1120:1137	64.57μAµmolL-1cm-2	1120:1137	64.57μAµmolL-1cm-2	1120:1137	The proposed biosensor demonstrated linearity in the range 0.199 - 16.22µmolL-1, with high sensitivity (64.57μAµmolL-1cm-2) and detection limit of 6.64 × 10-9molL-1 CPX.
29310303	4	55	from	improvement	959:969	arg1	sensitivity					974:984	sensitivity	974:984	sensitivity	974:984	The modified electrode showed an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE.
29310303	4	56	theme	electrochemical	836:850	arg1	activity					852:859	an excellent electrochemical activity	823:859	an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE	823:1013	The modified electrode showed an excellent electrochemical activity toward the electro-oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE.
29310303	1	57	theme	Au-In2O3	242:249	arg1	nanocomposite					251:263	Au-In2O3 nanocomposite	242:263	Au-In2O3 nanocomposite	242:263	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	3	58	theme	biosensor	646:654	arg1	Au--In2O3--CS/ABPE					666:683	the biosensor electrode Au--In2O3--CS/ABPE	642:683	the biosensor electrode Au--In2O3--CS/ABPE	642:683	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	7	59	theme	proposed	1336:1343	arg1	method					1345:1350	This proposed method	1331:1350	This proposed method	1331:1350	This proposed method was validated by UPLC and the results are in agreement at the 95% confidence level.
29310303	0	60	theme	black	80:84	arg1	electrode					92:100	acetylene black paste electrode	70:100	acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine	70:158	A novel sensor based on nanobiocomposite Au--In2O3--chitosan modified acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine.
29310303	3	61	theme	scanning	709:716	arg1	SEM					739:741	SEM	739:741	SEM	739:741	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	3	61	theme	scanning	709:716	arg1	microscopy					727:736	scanning electron microscopy	709:736	scanning electron microscopy (SEM)	709:742	Electrochemical activities and surface analysis of the biosensor electrode Au--In2O3--CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry.
29310303	0	62	theme	acetylene	70:78	arg1	electrode					92:100	acetylene black paste electrode	70:100	acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine	70:158	A novel sensor based on nanobiocomposite Au--In2O3--chitosan modified acetylene black paste electrode for sensitive detection of antimycotic ciclopirox olamine.
29310303	1	63	theme	acetylene	342:350	arg1	Au--In2O3--CS/ABPE					375:392	Au--In2O3--CS/ABPE	375:392	Au--In2O3--CS/ABPE	375:392	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	1	63	theme	acetylene	342:350	arg1	electrode					364:372	acetylene black paste electrode	342:372	acetylene black paste electrode (Au--In2O3--CS/ABPE)	342:393	For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au-In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au--In2O3--CS/ABPE).
29310303	6	64	with	formulations	1284:1295	arg1	precision					1320:1328	precision	1320:1328	precision	1320:1328	The analytical performance of this biosensor was evaluated for detection of CPX in pharmaceutical formulations with good accuracy and precision.
29310303	6	64	with	formulations	1284:1295	arg1	accuracy					1307:1314	good accuracy	1302:1314	good accuracy	1302:1314	The analytical performance of this biosensor was evaluated for detection of CPX in pharmaceutical formulations with good accuracy and precision.
27474671	5	0	theme	CNC	828:830	arg1	incorporation					811:823	The effective incorporation	797:823	The effective incorporation of CNC	797:830	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	5	1	theme	percolation	951:961	arg1	threshold					963:971	the percolation threshold	947:971	the percolation threshold	947:971	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	1	2	theme	high	302:305	arg1	properties					327:336	high specific mechanical properties	302:336	high specific mechanical properties	302:336	Focusing on eco-friendly materials, cellulose nanocrystals (CNC) have gained attention as nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties.
27474671	1	3	theme	due	207:209	arg1	nanoreinforcement					189:205	nanoreinforcement	189:205	nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties	189:336	Focusing on eco-friendly materials, cellulose nanocrystals (CNC) have gained attention as nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties.
27474671	1	4	theme	specific	307:314	arg1	properties					327:336	high specific mechanical properties	302:336	high specific mechanical properties	302:336	Focusing on eco-friendly materials, cellulose nanocrystals (CNC) have gained attention as nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties.
27474671	6	5	theme	agglomerates	1079:1090	arg1	presence					1067:1074	the presence	1063:1074	the presence of agglomerates	1063:1090	Nevertheless, above the percolation threshold, the presence of agglomerates reduced slightly these values.
27474671	7	6	theme	CNC	1189:1191	arg1	addition					1193:1200	CNC addition	1189:1200	CNC addition	1189:1200	The prepared nanocomposites showed increased hydrophilicity after CNC addition.
27474671	1	7	theme	mechanical	316:325	arg1	properties					327:336	high specific mechanical properties	302:336	high specific mechanical properties	302:336	Focusing on eco-friendly materials, cellulose nanocrystals (CNC) have gained attention as nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties.
27474671	5	8	from	stress	870:875	arg1	stability					927:935	an increased thermomechanical stability	897:935	an increased thermomechanical stability	897:935	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	5	9	dep	CNC	983:985	arg1	determined					990:999	determined	990:999	determined theoretically	990:1013	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	5	10	theme	%	981:981	arg1	CNC					983:985	a 3wt% CNC	976:985	a 3wt% CNC as determined theoretically	976:1013	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	1	11	theme	eco-friendly	111:122	arg1	materials					124:132	eco-friendly materials	111:132	eco-friendly materials	111:132	Focusing on eco-friendly materials, cellulose nanocrystals (CNC) have gained attention as nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties.
27474671	4	12	theme	CNC	738:740	arg1	content					742:748	different CNC content	728:748	different CNC content	728:748	Therefore, in this work a WBPU was synthesized for the preparation of nanocomposite films with different CNC content and the properties of the films were analyzed.
27474671	5	13	theme	increased	900:908	arg1	stability					927:935	an increased thermomechanical stability	897:935	an increased thermomechanical stability	897:935	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	5	14	theme	effective	801:809	arg1	incorporation					811:823	The effective incorporation	797:823	The effective incorporation of CNC	797:830	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	5	15	from	moduli	859:864	arg1	stability					927:935	an increased thermomechanical stability	897:935	an increased thermomechanical stability	897:935	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	3	16	theme	wide	605:608	arg1	range					610:614	a wide range	603:614	a wide range of applications	603:630	The possibility of tailoring the properties by varying the composition and nature of the reagents, opens the opportunity for a wide range of applications.
27474671	4	17	theme	films	717:721	arg1	preparation					688:698	the preparation	684:698	the preparation of nanocomposite films with different CNC content	684:748	Therefore, in this work a WBPU was synthesized for the preparation of nanocomposite films with different CNC content and the properties of the films were analyzed.
27474671	2	18	from	incorporation	428:440	arg1	WBPU					471:474	WBPU	471:474	WBPU	471:474	Furthermore, their water dispersibility makes them suitable nanoreinforcements for their incorporation in waterborne polyurethanes (WBPU).
27474671	2	18	from	incorporation	428:440	arg1	polyurethanes					456:468	waterborne polyurethanes	445:468	waterborne polyurethanes (WBPU)	445:475	Furthermore, their water dispersibility makes them suitable nanoreinforcements for their incorporation in waterborne polyurethanes (WBPU).
27474671	1	19	theme	exceptional	220:230	arg1	properties					232:241	their exceptional properties	214:241	their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties	214:336	Focusing on eco-friendly materials, cellulose nanocrystals (CNC) have gained attention as nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties.
27474671	0	20	theme	Cellulose	0:8	arg1	nanocrystals					10:21	Cellulose nanocrystals	0:21	Cellulose nanocrystals	0:21	Cellulose nanocrystals reinforced environmentally-friendly waterborne polyurethane nanocomposites.
27474671	7	21	theme	increased	1158:1166	arg1	hydrophilicity					1168:1181	increased hydrophilicity	1158:1181	increased hydrophilicity	1158:1181	The prepared nanocomposites showed increased hydrophilicity after CNC addition.
27474671	1	22	theme	cellulose	135:143	arg1	CNC					159:161	CNC	159:161	CNC	159:161	Focusing on eco-friendly materials, cellulose nanocrystals (CNC) have gained attention as nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties.
27474671	1	22	theme	cellulose	135:143	arg1	nanocrystals					145:156	cellulose nanocrystals	135:156	cellulose nanocrystals (CNC)	135:162	Focusing on eco-friendly materials, cellulose nanocrystals (CNC) have gained attention as nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties.
27474671	4	23	theme	different	728:736	arg1	content					742:748	different CNC content	728:748	different CNC content	728:748	Therefore, in this work a WBPU was synthesized for the preparation of nanocomposite films with different CNC content and the properties of the films were analyzed.
27474671	0	24	theme	environmentally-friendly	34:57	arg1	nanocomposites					83:96	environmentally-friendly waterborne polyurethane nanocomposites	34:96	environmentally-friendly waterborne polyurethane nanocomposites	34:96	Cellulose nanocrystals reinforced environmentally-friendly waterborne polyurethane nanocomposites.
27474671	7	25	theme	prepared	1127:1134	arg1	nanocomposites					1136:1149	The prepared nanocomposites	1123:1149	The prepared nanocomposites	1123:1149	The prepared nanocomposites showed increased hydrophilicity after CNC addition.
27474671	3	26	theme	applications	619:630	arg1	range					610:614	a wide range	603:614	a wide range of applications	603:630	The possibility of tailoring the properties by varying the composition and nature of the reagents, opens the opportunity for a wide range of applications.
27474671	2	27	dep	suitable	390:397	arg1	nanoreinforcements					399:416	nanoreinforcements	399:416	nanoreinforcements for their incorporation in waterborne polyurethanes (WBPU)	399:475	Furthermore, their water dispersibility makes them suitable nanoreinforcements for their incorporation in waterborne polyurethanes (WBPU).
27474671	2	28	theme	water	358:362	arg1	dispersibility					364:377	their water dispersibility	352:377	their water dispersibility	352:377	Furthermore, their water dispersibility makes them suitable nanoreinforcements for their incorporation in waterborne polyurethanes (WBPU).
27474671	0	29	theme	polyurethane	70:81	arg1	nanocomposites					83:96	environmentally-friendly waterborne polyurethane nanocomposites	34:96	environmentally-friendly waterborne polyurethane nanocomposites	34:96	Cellulose nanocrystals reinforced environmentally-friendly waterborne polyurethane nanocomposites.
27474671	4	30	theme	nanocomposite	703:715	arg1	films					717:721	nanocomposite films	703:721	nanocomposite films with different CNC content	703:748	Therefore, in this work a WBPU was synthesized for the preparation of nanocomposite films with different CNC content and the properties of the films were analyzed.
27474671	0	31	theme	waterborne	59:68	arg1	nanocomposites					83:96	environmentally-friendly waterborne polyurethane nanocomposites	34:96	environmentally-friendly waterborne polyurethane nanocomposites	34:96	Cellulose nanocrystals reinforced environmentally-friendly waterborne polyurethane nanocomposites.
27474671	1	32	theme	elevated	260:267	arg1	ratio					292:296	the elevated length/diameter aspect ratio	256:296	the elevated length/diameter aspect ratio	256:296	Focusing on eco-friendly materials, cellulose nanocrystals (CNC) have gained attention as nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties.
27474671	4	33	with	films	717:721	arg1	content					742:748	different CNC content	728:748	different CNC content	728:748	Therefore, in this work a WBPU was synthesized for the preparation of nanocomposite films with different CNC content and the properties of the films were analyzed.
27474671	3	34	theme	reagents	567:574	arg1	composition					537:547	composition	537:547	composition	537:547	The possibility of tailoring the properties by varying the composition and nature of the reagents, opens the opportunity for a wide range of applications.
27474671	3	34	theme	reagents	567:574	arg1	nature					553:558	nature	553:558	nature	553:558	The possibility of tailoring the properties by varying the composition and nature of the reagents, opens the opportunity for a wide range of applications.
27474671	5	35	from	yield	880:884	arg1	increase					847:854	an increase	844:854	an increase in moduli and stress at yield besides in an increased thermomechanical stability	844:935	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	5	35	from	yield	880:884	arg1	stress					870:875	stress	870:875	stress at yield besides in an increased thermomechanical stability	870:935	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	5	35	from	yield	880:884	arg1	moduli					859:864	moduli	859:864	moduli	859:864	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	5	36	theme	3wt	978:980	arg1	CNC					983:985	a 3wt% CNC	976:985	a 3wt% CNC as determined theoretically	976:1013	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	1	37	theme	length/diameter	269:283	arg1	ratio					292:296	the elevated length/diameter aspect ratio	256:296	the elevated length/diameter aspect ratio	256:296	Focusing on eco-friendly materials, cellulose nanocrystals (CNC) have gained attention as nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties.
27474671	3	38	dep	composition	537:547	arg1	the					533:535	the	533:535	the	533:535	The possibility of tailoring the properties by varying the composition and nature of the reagents, opens the opportunity for a wide range of applications.
27474671	4	39	theme	films	776:780	arg1	properties					758:767	the properties	754:767	the properties of the films	754:780	Therefore, in this work a WBPU was synthesized for the preparation of nanocomposite films with different CNC content and the properties of the films were analyzed.
27474671	1	40	theme	aspect	285:290	arg1	ratio					292:296	the elevated length/diameter aspect ratio	256:296	the elevated length/diameter aspect ratio	256:296	Focusing on eco-friendly materials, cellulose nanocrystals (CNC) have gained attention as nanoreinforcement due to their exceptional properties conferred by the elevated length/diameter aspect ratio and high specific mechanical properties.
27474671	5	41	theme	thermomechanical	910:925	arg1	stability					927:935	an increased thermomechanical stability	897:935	an increased thermomechanical stability	897:935	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	6	42	theme	percolation	1040:1050	arg1	threshold					1052:1060	the percolation threshold	1036:1060	the percolation threshold	1036:1060	Nevertheless, above the percolation threshold, the presence of agglomerates reduced slightly these values.
27474671	5	43	from	increase	847:854	arg1	yield					880:884	yield	880:884	yield besides	880:892	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	5	43	from	increase	847:854	arg1	stress					870:875	stress	870:875	stress at yield besides in an increased thermomechanical stability	870:935	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	5	43	from	increase	847:854	arg1	moduli					859:864	moduli	859:864	moduli	859:864	The effective incorporation of CNC resulted in an increase in moduli and stress at yield besides in an increased thermomechanical stability, reaching the percolation threshold at a 3wt% CNC as determined theoretically.
27474671	2	44	theme	waterborne	445:454	arg1	WBPU					471:474	WBPU	471:474	WBPU	471:474	Furthermore, their water dispersibility makes them suitable nanoreinforcements for their incorporation in waterborne polyurethanes (WBPU).
27474671	2	44	theme	waterborne	445:454	arg1	polyurethanes					456:468	waterborne polyurethanes	445:468	waterborne polyurethanes (WBPU)	445:475	Furthermore, their water dispersibility makes them suitable nanoreinforcements for their incorporation in waterborne polyurethanes (WBPU).
29048359	0	0	theme	Atrophic	81:88	arg1	Vaginitis					90:98	Atrophic Vaginitis	81:98	Atrophic Vaginitis	81:98	Chitosan Ascorbate Nanoparticles for the Vaginal Delivery of Antibiotic Drugs in Atrophic Vaginitis.
29048359	10	1	theme	mechanical	1505:1514	arg1	resistance					1516:1525	mechanical resistance	1505:1525	mechanical resistance	1505:1525	The optimized matrix was characterized by mechanical resistance and by the fast release in simulated vaginal fluid of nanoparticles characterized by unchanged size.
29048359	2	2	from	gelation	385:392	arg1	presence					401:408	the presence	397:408	the presence of pentasodium tripolyphosphate (TPP)	397:446	CSA NPs loaded with amoxicillin trihydrate (AX) were obtained by ionotropic gelation in the presence of pentasodium tripolyphosphate (TPP).
29048359	2	3	theme	amoxicillin	329:339	arg1	trihydrate					341:350	amoxicillin trihydrate	329:350	amoxicillin trihydrate (AX)	329:355	CSA NPs loaded with amoxicillin trihydrate (AX) were obtained by ionotropic gelation in the presence of pentasodium tripolyphosphate (TPP).
29048359	9	4	theme	antimicrobial	1367:1379	arg1	activity					1381:1388	an improved antimicrobial activity	1355:1388	an improved antimicrobial activity with respect to a chitosan solution, prepared at the same concentration	1355:1460	In particular, nanoparticles were characterized by an improved antimicrobial activity with respect to a chitosan solution, prepared at the same concentration.
29048359	7	5	theme	suitable	1039:1046	arg1	resistance					1028:1037	a mechanical resistance	1015:1037	a mechanical resistance suitable for the administration into the vaginal cavity	1015:1093	They should possess a mechanical resistance suitable for the administration into the vaginal cavity and should readily dissolve in the vaginal fluid.
29048359	5	6	theme	simplex	835:841	arg1	approach					825:832	a DoE (Design of Experiments) approach	795:832	a DoE (Design of Experiments) approach (simplex centroid design)	795:858	Subsequently, CSA NPs were loaded in polymeric matrices, whose composition was optimized using a DoE (Design of Experiments) approach (simplex centroid design).
29048359	5	6	theme	simplex	835:841	arg1	design					852:857	simplex centroid design	835:857	simplex centroid design	835:857	Subsequently, CSA NPs were loaded in polymeric matrices, whose composition was optimized using a DoE (Design of Experiments) approach (simplex centroid design).
29048359	5	7	theme	centroid	843:850	arg1	approach					825:832	a DoE (Design of Experiments) approach	795:832	a DoE (Design of Experiments) approach (simplex centroid design)	795:858	Subsequently, CSA NPs were loaded in polymeric matrices, whose composition was optimized using a DoE (Design of Experiments) approach (simplex centroid design).
29048359	5	7	theme	centroid	843:850	arg1	design					852:857	simplex centroid design	835:857	simplex centroid design	835:857	Subsequently, CSA NPs were loaded in polymeric matrices, whose composition was optimized using a DoE (Design of Experiments) approach (simplex centroid design).
29048359	9	8	theme	same	1443:1446	arg1	concentration					1448:1460	the same concentration	1439:1460	the same concentration	1439:1460	In particular, nanoparticles were characterized by an improved antimicrobial activity with respect to a chitosan solution, prepared at the same concentration.
29048359	10	9	theme	optimized	1467:1475	arg1	matrix					1477:1482	The optimized matrix	1463:1482	The optimized matrix	1463:1482	The optimized matrix was characterized by mechanical resistance and by the fast release in simulated vaginal fluid of nanoparticles characterized by unchanged size.
29048359	6	10	theme	excipients	940:949	arg1	solutions					909:917	aqueous solutions	901:917	aqueous solutions of three hydrophilic excipients, polyvinylpirrolidone, mannitol and glycin	901:992	Matrices were obtained by freeze-drying aqueous solutions of three hydrophilic excipients, polyvinylpirrolidone, mannitol and glycin.
29048359	10	11	theme	fast	1538:1541	arg1	release					1543:1549	the fast release	1534:1549	the fast release in simulated vaginal fluid of nanoparticles characterized by unchanged size	1534:1625	The optimized matrix was characterized by mechanical resistance and by the fast release in simulated vaginal fluid of nanoparticles characterized by unchanged size.
29048359	7	12	theme	vaginal	1080:1086	arg1	cavity					1088:1093	the vaginal cavity	1076:1093	the vaginal cavity	1076:1093	They should possess a mechanical resistance suitable for the administration into the vaginal cavity and should readily dissolve in the vaginal fluid.
29048359	10	13	theme	nanoparticles	1581:1593	arg1	fluid					1572:1576	simulated vaginal fluid	1554:1576	simulated vaginal fluid of nanoparticles characterized by unchanged size	1554:1625	The optimized matrix was characterized by mechanical resistance and by the fast release in simulated vaginal fluid of nanoparticles characterized by unchanged size.
29048359	4	14	theme	antimicrobial	674:686	arg1	properties					688:697	antimicrobial properties	674:697	antimicrobial properties	674:697	CSA NPs were characterized for mucoadhesive, wound healing and antimicrobial properties.
29048359	3	15	theme	Different	449:457	arg1	CSA					459:461	Different CSA:TPP and CSA	449:473	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.	449:609	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	0	16	from	Delivery	49:56	arg1	Vaginitis					90:98	Atrophic Vaginitis	81:98	Atrophic Vaginitis	81:98	Chitosan Ascorbate Nanoparticles for the Vaginal Delivery of Antibiotic Drugs in Atrophic Vaginitis.
29048359	3	17	theme	AX	475:476	arg1	ratios					485:490	AX weight ratios	475:490	AX weight ratios	475:490	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	5	18	theme	Experiments	812:822	arg1	Design					802:807	Design	802:807	Design of Experiments	802:822	Subsequently, CSA NPs were loaded in polymeric matrices, whose composition was optimized using a DoE (Design of Experiments) approach (simplex centroid design).
29048359	1	19	theme	chitosan	152:159	arg1	nanoparticles					171:183	chitosan ascorbate nanoparticles	152:183	chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis	152:306	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	1	19	theme	chitosan	152:159	arg1	NPs					190:192	CSA NPs	186:192	CSA NPs	186:192	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	6	20	theme	hydrophilic	928:938	arg1	mannitol					974:981	mannitol	974:981	mannitol	974:981	Matrices were obtained by freeze-drying aqueous solutions of three hydrophilic excipients, polyvinylpirrolidone, mannitol and glycin.
29048359	6	20	theme	hydrophilic	928:938	arg1	polyvinylpirrolidone					952:971	polyvinylpirrolidone	952:971	polyvinylpirrolidone	952:971	Matrices were obtained by freeze-drying aqueous solutions of three hydrophilic excipients, polyvinylpirrolidone, mannitol and glycin.
29048359	6	20	theme	hydrophilic	928:938	arg1	excipients					940:949	three hydrophilic excipients	922:949	three hydrophilic excipients	922:949	Matrices were obtained by freeze-drying aqueous solutions of three hydrophilic excipients, polyvinylpirrolidone, mannitol and glycin.
29048359	6	20	theme	hydrophilic	928:938	arg1	glycin					987:992	glycin	987:992	glycin	987:992	Matrices were obtained by freeze-drying aqueous solutions of three hydrophilic excipients, polyvinylpirrolidone, mannitol and glycin.
29048359	2	21	dep	trihydrate	341:350	arg1	AX					353:354	AX	353:354	AX	353:354	CSA NPs loaded with amoxicillin trihydrate (AX) were obtained by ionotropic gelation in the presence of pentasodium tripolyphosphate (TPP).
29048359	7	22	contain	possess	1007:1013	arg2	resistance					1028:1037	a mechanical resistance	1015:1037	a mechanical resistance suitable for the administration into the vaginal cavity	1015:1093	They should possess a mechanical resistance suitable for the administration into the vaginal cavity and should readily dissolve in the vaginal fluid.
29048359	7	22	contain	possess	1007:1013	arg1	They					995:998	They	995:998	They	995:998	They should possess a mechanical resistance suitable for the administration into the vaginal cavity and should readily dissolve in the vaginal fluid.
29048359	1	23	theme	ascorbate	161:169	arg1	nanoparticles					171:183	chitosan ascorbate nanoparticles	152:183	chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis	152:306	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	1	23	theme	ascorbate	161:169	arg1	NPs					190:192	CSA NPs	186:192	CSA NPs	186:192	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	2	24	theme	pentasodium	413:423	arg1	TPP					443:445	TPP	443:445	TPP	443:445	CSA NPs loaded with amoxicillin trihydrate (AX) were obtained by ionotropic gelation in the presence of pentasodium tripolyphosphate (TPP).
29048359	2	24	theme	pentasodium	413:423	arg1	tripolyphosphate					425:440	pentasodium tripolyphosphate	413:440	pentasodium tripolyphosphate (TPP)	413:446	CSA NPs loaded with amoxicillin trihydrate (AX) were obtained by ionotropic gelation in the presence of pentasodium tripolyphosphate (TPP).
29048359	10	25	theme	vaginal	1564:1570	arg1	fluid					1572:1576	simulated vaginal fluid	1554:1576	simulated vaginal fluid of nanoparticles characterized by unchanged size	1554:1625	The optimized matrix was characterized by mechanical resistance and by the fast release in simulated vaginal fluid of nanoparticles characterized by unchanged size.
29048359	5	26	theme	CSA	714:716	arg1	NPs					718:720	CSA NPs	714:720	CSA NPs	714:720	Subsequently, CSA NPs were loaded in polymeric matrices, whose composition was optimized using a DoE (Design of Experiments) approach (simplex centroid design).
29048359	1	27	theme	nanoparticles	171:183	arg1	development					137:147	the development	133:147	the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis	133:306	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	1	27	theme	nanoparticles	171:183	arg1	aim					105:107	The aim	101:107	The aim of the present work	101:127	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	0	28	theme	Chitosan	0:7	arg1	Ascorbate					9:17	Chitosan Ascorbate	0:17	Chitosan Ascorbate	0:17	Chitosan Ascorbate Nanoparticles for the Vaginal Delivery of Antibiotic Drugs in Atrophic Vaginitis.
29048359	1	29	theme	antibiotic	252:261	arg1	drugs					263:267	antibiotic drugs	252:267	antibiotic drugs	252:267	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	10	30	theme	unchanged	1612:1620	arg1	size					1622:1625	unchanged size	1612:1625	unchanged size	1612:1625	The optimized matrix was characterized by mechanical resistance and by the fast release in simulated vaginal fluid of nanoparticles characterized by unchanged size.
29048359	8	31	theme	antioxidant	1160:1170	arg1	properties					1172:1181	antioxidant properties	1160:1181	antioxidant properties	1160:1181	In addition to antioxidant properties, due to the presence of ascorbic acid, CSA NPs showed in vitro mucoadhesive, wound healing and antimicrobial properties.
29048359	1	32	theme	drugs	263:267	arg1	delivery					240:247	the delivery	236:247	the delivery of antibiotic drugs in the treatment of atrophic vaginitis	236:306	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	1	33	from	delivery	240:247	arg1	treatment					276:284	the treatment	272:284	the treatment of atrophic vaginitis	272:306	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	1	34	theme	CSA	186:188	arg1	nanoparticles					171:183	chitosan ascorbate nanoparticles	152:183	chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis	152:306	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	1	34	theme	CSA	186:188	arg1	NPs					190:192	CSA NPs	186:192	CSA NPs	186:192	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	7	35	theme	vaginal	1130:1136	arg1	fluid					1138:1142	the vaginal fluid	1126:1142	the vaginal fluid	1126:1142	They should possess a mechanical resistance suitable for the administration into the vaginal cavity and should readily dissolve in the vaginal fluid.
29048359	3	36	dep	CSA	459:461	arg1	investigated					597:608	investigated	597:608	were investigated	592:608	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	3	36	dep	CSA	459:461	arg1	CSA					471:473	CSA	471:473	CSA	471:473	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	3	36	dep	CSA	459:461	arg1	considered					497:506	considered	497:506	were considered	492:506	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	3	36	dep	CSA	459:461	arg1	TPP					463:465	TPP	463:465	TPP	463:465	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	8	37	theme	acid	1216:1219	arg1	presence					1195:1202	the presence	1191:1202	the presence of ascorbic acid	1191:1219	In addition to antioxidant properties, due to the presence of ascorbic acid, CSA NPs showed in vitro mucoadhesive, wound healing and antimicrobial properties.
29048359	0	38	theme	Vaginal	41:47	arg1	Delivery					49:56	the Vaginal Delivery	37:56	the Vaginal Delivery of Antibiotic Drugs in Atrophic Vaginitis	37:98	Chitosan Ascorbate Nanoparticles for the Vaginal Delivery of Antibiotic Drugs in Atrophic Vaginitis.
29048359	6	39	theme	aqueous	901:907	arg1	solutions					909:917	aqueous solutions	901:917	aqueous solutions of three hydrophilic excipients, polyvinylpirrolidone, mannitol and glycin	901:992	Matrices were obtained by freeze-drying aqueous solutions of three hydrophilic excipients, polyvinylpirrolidone, mannitol and glycin.
29048359	5	40	theme	DoE	797:799	arg1	approach					825:832	a DoE (Design of Experiments) approach	795:832	a DoE (Design of Experiments) approach (simplex centroid design)	795:858	Subsequently, CSA NPs were loaded in polymeric matrices, whose composition was optimized using a DoE (Design of Experiments) approach (simplex centroid design).
29048359	5	40	theme	DoE	797:799	arg1	design					852:857	simplex centroid design	835:857	simplex centroid design	835:857	Subsequently, CSA NPs were loaded in polymeric matrices, whose composition was optimized using a DoE (Design of Experiments) approach (simplex centroid design).
29048359	3	41	theme	particle	535:542	arg1	size					544:547	the particle size	531:547	the particle size	531:547	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	2	42	theme	ionotropic	374:383	arg1	gelation					385:392	ionotropic gelation	374:392	ionotropic gelation in the presence of pentasodium tripolyphosphate (TPP)	374:446	CSA NPs loaded with amoxicillin trihydrate (AX) were obtained by ionotropic gelation in the presence of pentasodium tripolyphosphate (TPP).
29048359	4	43	theme	CSA	611:613	arg1	NPs					615:617	CSA NPs	611:617	CSA NPs	611:617	CSA NPs were characterized for mucoadhesive, wound healing and antimicrobial properties.
29048359	5	44	theme	polymeric	737:745	arg1	matrices					747:754	polymeric matrices	737:754	polymeric matrices	737:754	Subsequently, CSA NPs were loaded in polymeric matrices, whose composition was optimized using a DoE (Design of Experiments) approach (simplex centroid design).
29048359	3	45	theme	production	575:584	arg1	yield					586:590	production yield	575:590	production yield	575:590	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	10	46	theme	simulated	1554:1562	arg1	fluid					1572:1576	simulated vaginal fluid	1554:1576	simulated vaginal fluid of nanoparticles characterized by unchanged size	1554:1625	The optimized matrix was characterized by mechanical resistance and by the fast release in simulated vaginal fluid of nanoparticles characterized by unchanged size.
29048359	8	47	theme	CSA	1222:1224	arg1	NPs					1226:1228	CSA NPs	1222:1228	CSA NPs	1222:1228	In addition to antioxidant properties, due to the presence of ascorbic acid, CSA NPs showed in vitro mucoadhesive, wound healing and antimicrobial properties.
29048359	8	48	theme	in	1237:1238	arg1	healing					1266:1272	wound healing	1260:1272	wound healing	1260:1272	In addition to antioxidant properties, due to the presence of ascorbic acid, CSA NPs showed in vitro mucoadhesive, wound healing and antimicrobial properties.
29048359	7	49	theme	mechanical	1017:1026	arg1	resistance					1028:1037	a mechanical resistance	1015:1037	a mechanical resistance suitable for the administration into the vaginal cavity	1015:1093	They should possess a mechanical resistance suitable for the administration into the vaginal cavity and should readily dissolve in the vaginal fluid.
29048359	2	50	theme	tripolyphosphate	425:440	arg1	presence					401:408	the presence	397:408	the presence of pentasodium tripolyphosphate (TPP)	397:446	CSA NPs loaded with amoxicillin trihydrate (AX) were obtained by ionotropic gelation in the presence of pentasodium tripolyphosphate (TPP).
29048359	1	51	theme	atrophic	289:296	arg1	vaginitis					298:306	atrophic vaginitis	289:306	atrophic vaginitis	289:306	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	4	52	theme	wound	656:660	arg1	healing					662:668	wound healing	656:668	wound healing	656:668	CSA NPs were characterized for mucoadhesive, wound healing and antimicrobial properties.
29048359	1	53	theme	vaginitis	298:306	arg1	treatment					276:284	the treatment	272:284	the treatment of atrophic vaginitis	272:306	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	5	54	dep	DoE	797:799	arg1	Design					802:807	Design	802:807	Design of Experiments	802:822	Subsequently, CSA NPs were loaded in polymeric matrices, whose composition was optimized using a DoE (Design of Experiments) approach (simplex centroid design).
29048359	8	55	theme	ascorbic	1207:1214	arg1	acid					1216:1219	ascorbic acid	1207:1219	ascorbic acid	1207:1219	In addition to antioxidant properties, due to the presence of ascorbic acid, CSA NPs showed in vitro mucoadhesive, wound healing and antimicrobial properties.
29048359	1	56	theme	present	116:122	arg1	work					124:127	the present work	112:127	the present work	112:127	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	3	57	theme	weight	478:483	arg1	ratios					485:490	AX weight ratios	475:490	AX weight ratios	475:490	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	10	58	from	release	1543:1549	arg1	fluid					1572:1576	simulated vaginal fluid	1554:1576	simulated vaginal fluid of nanoparticles characterized by unchanged size	1554:1625	The optimized matrix was characterized by mechanical resistance and by the fast release in simulated vaginal fluid of nanoparticles characterized by unchanged size.
29048359	1	59	theme	work	124:127	arg1	development					137:147	the development	133:147	the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis	133:306	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	1	59	theme	work	124:127	arg1	aim					105:107	The aim	101:107	The aim of the present work	101:127	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	8	60	theme	antimicrobial	1278:1290	arg1	properties					1292:1301	antimicrobial properties	1278:1301	antimicrobial properties	1278:1301	In addition to antioxidant properties, due to the presence of ascorbic acid, CSA NPs showed in vitro mucoadhesive, wound healing and antimicrobial properties.
29048359	2	61	theme	CSA	309:311	arg1	NPs					313:315	CSA NPs	309:315	CSA NPs loaded with amoxicillin trihydrate (AX)	309:355	CSA NPs loaded with amoxicillin trihydrate (AX) were obtained by ionotropic gelation in the presence of pentasodium tripolyphosphate (TPP).
29048359	0	62	theme	Drugs	72:76	arg1	Delivery					49:56	the Vaginal Delivery	37:56	the Vaginal Delivery of Antibiotic Drugs in Atrophic Vaginitis	37:98	Chitosan Ascorbate Nanoparticles for the Vaginal Delivery of Antibiotic Drugs in Atrophic Vaginitis.
29048359	8	63	dep	in	1237:1238	arg1	vitro					1240:1244	vitro	1240:1244	vitro	1240:1244	In addition to antioxidant properties, due to the presence of ascorbic acid, CSA NPs showed in vitro mucoadhesive, wound healing and antimicrobial properties.
29048359	8	63	dep	in	1237:1238	arg1	mucoadhesive					1246:1257	mucoadhesive	1246:1257	mucoadhesive	1246:1257	In addition to antioxidant properties, due to the presence of ascorbic acid, CSA NPs showed in vitro mucoadhesive, wound healing and antimicrobial properties.
29048359	8	64	theme	wound	1260:1264	arg1	healing					1266:1272	wound healing	1260:1272	wound healing	1260:1272	In addition to antioxidant properties, due to the presence of ascorbic acid, CSA NPs showed in vitro mucoadhesive, wound healing and antimicrobial properties.
29048359	3	65	from	influence	518:526	arg1	size					544:547	the particle size	531:547	the particle size	531:547	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	3	65	from	influence	518:526	arg1	index					565:569	polydispersion index	550:569	polydispersion index	550:569	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	3	65	from	influence	518:526	arg1	yield					586:590	production yield	575:590	production yield	575:590	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	1	66	theme	fast-dissolving	209:223	arg1	matrix					225:230	a fast-dissolving matrix	207:230	a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis	207:306	The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis.
29048359	0	67	theme	Antibiotic	61:70	arg1	Drugs					72:76	Antibiotic Drugs	61:76	Antibiotic Drugs	61:76	Chitosan Ascorbate Nanoparticles for the Vaginal Delivery of Antibiotic Drugs in Atrophic Vaginitis.
29048359	9	68	theme	chitosan	1408:1415	arg1	solution					1417:1424	a chitosan solution	1406:1424	a chitosan solution	1406:1424	In particular, nanoparticles were characterized by an improved antimicrobial activity with respect to a chitosan solution, prepared at the same concentration.
29048359	3	69	theme	polydispersion	550:563	arg1	index					565:569	polydispersion index	550:569	polydispersion index	550:569	Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated.
29048359	9	70	theme	improved	1358:1365	arg1	activity					1381:1388	an improved antimicrobial activity	1355:1388	an improved antimicrobial activity with respect to a chitosan solution, prepared at the same concentration	1355:1460	In particular, nanoparticles were characterized by an improved antimicrobial activity with respect to a chitosan solution, prepared at the same concentration.
27542483	4	0	theme	monosaccharides	473:487	arg1	ratio					446:450	The ratio	442:450	The ratio of neutral to acidic monosaccharides	442:487	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	1	dep	5.7	511:513	arg1	to					508:509	to	508:509	to	508:509	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	2	theme	samples	805:811	arg1	FAC					794:796	FAC	794:796	FAC	794:796	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	2	theme	samples	805:811	arg1	zeta-potential					628:641	zeta-potential	628:641	zeta-potential	628:641	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	2	theme	samples	805:811	arg1	index					685:689	emulsifying activity index	664:689	emulsifying activity index (EAI)	664:695	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	2	theme	samples	805:811	arg1	capacity					750:757	water absorption capacity	733:757	water absorption capacity (WAC)	733:763	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	2	theme	samples	805:811	arg1	index					720:724	emulsion stability index	701:724	emulsion stability index	701:724	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	2	theme	samples	805:811	arg1	EAI					692:694	EAI	692:694	EAI	692:694	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	2	theme	samples	805:811	arg1	capacity					784:791	fat absorption capacity	769:791	fat absorption capacity (FAC) of FG samples	769:811	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	2	theme	samples	805:811	arg1	ESI					727:729	ESI	727:729	ESI	727:729	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	2	theme	samples	805:811	arg1	morphology					652:661	surface morphology	644:661	surface morphology	644:661	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	2	theme	samples	805:811	arg1	WAC					760:762	WAC	760:762	WAC	760:762	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	0	3	theme	gum	97:99	arg1	properties					74:83	functional properties	63:83	functional properties	63:83	Effect of extraction temperature on composition, structure and functional properties of flaxseed gum.
27542483	0	3	theme	gum	97:99	arg1	composition					36:46	composition	36:46	composition	36:46	Effect of extraction temperature on composition, structure and functional properties of flaxseed gum.
27542483	0	3	theme	gum	97:99	arg1	structure					49:57	structure	49:57	structure	49:57	Effect of extraction temperature on composition, structure and functional properties of flaxseed gum.
27542483	0	4	from	Effect	0:5	arg1	properties					74:83	functional properties	63:83	functional properties	63:83	Effect of extraction temperature on composition, structure and functional properties of flaxseed gum.
27542483	0	4	from	Effect	0:5	arg1	composition					36:46	composition	36:46	composition	36:46	Effect of extraction temperature on composition, structure and functional properties of flaxseed gum.
27542483	0	4	from	Effect	0:5	arg1	structure					49:57	structure	49:57	structure	49:57	Effect of extraction temperature on composition, structure and functional properties of flaxseed gum.
27542483	4	5	theme	water	733:737	arg1	WAC					760:762	WAC	760:762	WAC	760:762	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	5	theme	water	733:737	arg1	capacity					750:757	water absorption capacity	733:757	water absorption capacity (WAC)	733:763	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	0	6	theme	flaxseed	88:95	arg1	gum					97:99	flaxseed gum	88:99	flaxseed gum	88:99	Effect of extraction temperature on composition, structure and functional properties of flaxseed gum.
27542483	4	7	theme	neutral	455:461	arg1	monosaccharides					473:487	neutral to acidic monosaccharides	455:487	neutral to acidic monosaccharides	455:487	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	5	8	theme	samples	896:902	arg1	WAC					886:888	WAC	886:888	WAC	886:888	EAI and WAC of FG samples reduced significantly with rise in extraction temperature.
27542483	5	8	theme	samples	896:902	arg1	EAI					878:880	EAI	878:880	EAI	878:880	EAI and WAC of FG samples reduced significantly with rise in extraction temperature.
27542483	2	9	theme	FG	236:237	arg1	composition					197:207	Chemical composition	188:207	Chemical composition	188:207	Chemical composition and structural features of FG extracted at different temperatures were investigated to determine the effect of temperature.
27542483	2	9	theme	FG	236:237	arg1	features					224:231	structural features	213:231	structural features	213:231	Chemical composition and structural features of FG extracted at different temperatures were investigated to determine the effect of temperature.
27542483	6	10	from	formation	1117:1125	arg1	systems					1135:1141	food systems	1130:1141	food systems	1130:1141	Our study suggests that FG extracted at different temperatures may be specifically targeted for different applications, such as for emulsification or gel formation in food systems.
27542483	4	11	theme	emulsion	701:708	arg1	index					720:724	emulsion stability index	701:724	emulsion stability index	701:724	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	6	12	theme	different	1003:1011	arg1	temperatures					1013:1024	different temperatures	1003:1024	different temperatures	1003:1024	Our study suggests that FG extracted at different temperatures may be specifically targeted for different applications, such as for emulsification or gel formation in food systems.
27542483	1	13	theme	different	142:150	arg1	temperatures					152:163	four different temperatures	137:163	four different temperatures (30, 50, 70 and 90°C)	137:185	Flaxseed gum (FG) was extracted at four different temperatures (30, 50, 70 and 90°C).
27542483	4	14	theme	acidic	466:471	arg1	monosaccharides					473:487	neutral to acidic monosaccharides	455:487	neutral to acidic monosaccharides	455:487	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	15	theme	absorption	739:748	arg1	WAC					760:762	WAC	760:762	WAC	760:762	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	15	theme	absorption	739:748	arg1	capacity					750:757	water absorption capacity	733:757	water absorption capacity (WAC)	733:763	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	3	16	theme	extraction	418:427	arg1	temperature					429:439	FG extraction temperature	415:439	FG extraction temperature	415:439	Content of acidic monosaccharides and denatured protein increased with increasing FG extraction temperature.
27542483	3	17	theme	acidic	344:349	arg1	monosaccharides					351:365	acidic monosaccharides	344:365	acidic monosaccharides	344:365	Content of acidic monosaccharides and denatured protein increased with increasing FG extraction temperature.
27542483	4	18	theme	FG	802:803	arg1	samples					805:811	FG samples	802:811	FG samples	802:811	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	5	19	from	rise	931:934	arg1	temperature					950:960	extraction temperature	939:960	extraction temperature	939:960	EAI and WAC of FG samples reduced significantly with rise in extraction temperature.
27542483	4	20	theme	emulsifying	664:674	arg1	ESI					727:729	ESI	727:729	ESI	727:729	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	20	theme	emulsifying	664:674	arg1	EAI					692:694	EAI	692:694	EAI	692:694	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	20	theme	emulsifying	664:674	arg1	index					685:689	emulsifying activity index	664:689	emulsifying activity index (EAI)	664:695	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	3	21	theme	monosaccharides	351:365	arg1	Content					333:339	Content	333:339	Content of acidic monosaccharides and denatured protein	333:387	Content of acidic monosaccharides and denatured protein increased with increasing FG extraction temperature.
27542483	0	22	theme	temperature	21:31	arg1	Effect					0:5	Effect	0:5	Effect of extraction temperature on composition, structure and functional properties of flaxseed gum.	0:100	Effect of extraction temperature on composition, structure and functional properties of flaxseed gum.
27542483	4	23	theme	extraction	522:531	arg1	temperature					533:543	the extraction temperature	518:543	the extraction temperature	518:543	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	24	theme	extraction	854:863	arg1	temperature					865:875	extraction temperature	854:875	extraction temperature	854:875	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	2	25	theme	different	252:260	arg1	temperatures					262:273	different temperatures	252:273	different temperatures	252:273	Chemical composition and structural features of FG extracted at different temperatures were investigated to determine the effect of temperature.
27542483	0	26	theme	extraction	10:19	arg1	temperature					21:31	extraction temperature	10:31	extraction temperature	10:31	Effect of extraction temperature on composition, structure and functional properties of flaxseed gum.
27542483	6	27	theme	food	1130:1133	arg1	systems					1135:1141	food systems	1130:1141	food systems	1130:1141	Our study suggests that FG extracted at different temperatures may be specifically targeted for different applications, such as for emulsification or gel formation in food systems.
27542483	4	28	dep	90°C.	570:574	arg1	investigated					824:835	investigated	824:835	were also investigated as a function of extraction temperature	814:875	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	1	29	theme	Flaxseed	102:109	arg1	FG					116:117	FG	116:117	FG	116:117	Flaxseed gum (FG) was extracted at four different temperatures (30, 50, 70 and 90°C).
27542483	1	29	theme	Flaxseed	102:109	arg1	gum					111:113	Flaxseed gum	102:113	Flaxseed gum (FG)	102:118	Flaxseed gum (FG) was extracted at four different temperatures (30, 50, 70 and 90°C).
27542483	3	30	theme	protein	381:387	arg1	Content					333:339	Content	333:339	Content of acidic monosaccharides and denatured protein	333:387	Content of acidic monosaccharides and denatured protein increased with increasing FG extraction temperature.
27542483	5	31	theme	extraction	939:948	arg1	temperature					950:960	extraction temperature	939:960	extraction temperature	939:960	EAI and WAC of FG samples reduced significantly with rise in extraction temperature.
27542483	4	32	theme	absorption	773:782	arg1	FAC					794:796	FAC	794:796	FAC	794:796	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	32	theme	absorption	773:782	arg1	capacity					784:791	fat absorption capacity	769:791	fat absorption capacity (FAC) of FG samples	769:811	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	6	33	from	emulsification	1095:1108	arg1	systems					1135:1141	food systems	1130:1141	food systems	1130:1141	Our study suggests that FG extracted at different temperatures may be specifically targeted for different applications, such as for emulsification or gel formation in food systems.
27542483	3	34	theme	FG	415:416	arg1	temperature					429:439	FG extraction temperature	415:439	FG extraction temperature	415:439	Content of acidic monosaccharides and denatured protein increased with increasing FG extraction temperature.
27542483	4	35	theme	temperature	865:875	arg1	zeta-potential					628:641	zeta-potential	628:641	zeta-potential	628:641	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	35	theme	temperature	865:875	arg1	index					685:689	emulsifying activity index	664:689	emulsifying activity index (EAI)	664:695	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	35	theme	temperature	865:875	arg1	capacity					750:757	water absorption capacity	733:757	water absorption capacity (WAC)	733:763	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	35	theme	temperature	865:875	arg1	function					842:849	a function	840:849	a function of extraction temperature	840:875	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	35	theme	temperature	865:875	arg1	capacity					784:791	fat absorption capacity	769:791	fat absorption capacity (FAC) of FG samples	769:811	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	35	theme	temperature	865:875	arg1	properties					606:615	Physiochemical and functional properties	576:615	properties	606:615	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	35	theme	temperature	865:875	arg1	morphology					652:661	surface morphology	644:661	surface morphology	644:661	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	36	theme	fat	769:771	arg1	FAC					794:796	FAC	794:796	FAC	794:796	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	36	theme	fat	769:771	arg1	capacity					784:791	fat absorption capacity	769:791	fat absorption capacity (FAC) of FG samples	769:811	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	3	37	theme	denatured	371:379	arg1	protein					381:387	denatured protein	371:387	denatured protein	371:387	Content of acidic monosaccharides and denatured protein increased with increasing FG extraction temperature.
27542483	1	38	dep	temperatures	152:163	arg1	90°C					181:184	90°C	181:184	90°C	181:184	Flaxseed gum (FG) was extracted at four different temperatures (30, 50, 70 and 90°C).
27542483	1	38	dep	temperatures	152:163	arg1	70					174:175	70	174:175	70	174:175	Flaxseed gum (FG) was extracted at four different temperatures (30, 50, 70 and 90°C).
27542483	4	39	dep	monosaccharides	473:487	arg1	to					463:464	to	463:464	to	463:464	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	2	40	theme	structural	213:222	arg1	features					224:231	structural features	213:231	structural features	213:231	Chemical composition and structural features of FG extracted at different temperatures were investigated to determine the effect of temperature.
27542483	4	41	theme	stability	710:718	arg1	index					720:724	emulsion stability index	701:724	emulsion stability index	701:724	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	6	42	theme	different	1059:1067	arg1	applications					1069:1080	different applications	1059:1080	different applications	1059:1080	Our study suggests that FG extracted at different temperatures may be specifically targeted for different applications, such as for emulsification or gel formation in food systems.
27542483	6	43	theme	gel	1113:1115	arg1	formation					1117:1125	gel formation	1113:1125	gel formation	1113:1125	Our study suggests that FG extracted at different temperatures may be specifically targeted for different applications, such as for emulsification or gel formation in food systems.
27542483	5	44	theme	FG	893:894	arg1	samples					896:902	FG samples	893:902	FG samples	893:902	EAI and WAC of FG samples reduced significantly with rise in extraction temperature.
27542483	4	45	theme	activity	676:683	arg1	ESI					727:729	ESI	727:729	ESI	727:729	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	45	theme	activity	676:683	arg1	EAI					692:694	EAI	692:694	EAI	692:694	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	45	theme	activity	676:683	arg1	index					685:689	emulsifying activity index	664:689	emulsifying activity index (EAI)	664:695	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	46	theme	Physiochemical	576:589	arg1	zeta-potential					628:641	zeta-potential	628:641	zeta-potential	628:641	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	46	theme	Physiochemical	576:589	arg1	index					685:689	emulsifying activity index	664:689	emulsifying activity index (EAI)	664:695	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	46	theme	Physiochemical	576:589	arg1	capacity					750:757	water absorption capacity	733:757	water absorption capacity (WAC)	733:763	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	46	theme	Physiochemical	576:589	arg1	function					842:849	a function	840:849	a function of extraction temperature	840:875	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	46	theme	Physiochemical	576:589	arg1	capacity					784:791	fat absorption capacity	769:791	fat absorption capacity (FAC) of FG samples	769:811	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	46	theme	Physiochemical	576:589	arg1	properties					606:615	Physiochemical and functional properties	576:615	properties	606:615	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	46	theme	Physiochemical	576:589	arg1	morphology					652:661	surface morphology	644:661	surface morphology	644:661	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	0	47	theme	functional	63:72	arg1	properties					74:83	functional properties	63:83	functional properties	63:83	Effect of extraction temperature on composition, structure and functional properties of flaxseed gum.
27542483	6	48	dep	such	1083:1086	arg1	as					1088:1089	as	1088:1089	as	1088:1089	Our study suggests that FG extracted at different temperatures may be specifically targeted for different applications, such as for emulsification or gel formation in food systems.
27542483	4	49	theme	surface	644:650	arg1	morphology					652:661	surface morphology	644:661	surface morphology	644:661	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	2	50	theme	Chemical	188:195	arg1	composition					197:207	Chemical composition	188:207	Chemical composition	188:207	Chemical composition and structural features of FG extracted at different temperatures were investigated to determine the effect of temperature.
27542483	2	51	theme	temperature	320:330	arg1	effect					310:315	the effect	306:315	the effect of temperature	306:330	Chemical composition and structural features of FG extracted at different temperatures were investigated to determine the effect of temperature.
27542483	4	52	theme	functional	595:604	arg1	zeta-potential					628:641	zeta-potential	628:641	zeta-potential	628:641	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	52	theme	functional	595:604	arg1	index					685:689	emulsifying activity index	664:689	emulsifying activity index (EAI)	664:695	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	52	theme	functional	595:604	arg1	capacity					750:757	water absorption capacity	733:757	water absorption capacity (WAC)	733:763	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	52	theme	functional	595:604	arg1	function					842:849	a function	840:849	a function of extraction temperature	840:875	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	52	theme	functional	595:604	arg1	capacity					784:791	fat absorption capacity	769:791	fat absorption capacity (FAC) of FG samples	769:811	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	52	theme	functional	595:604	arg1	properties					606:615	Physiochemical and functional properties	576:615	properties	606:615	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
27542483	4	52	theme	functional	595:604	arg1	morphology					652:661	surface morphology	644:661	surface morphology	644:661	The ratio of neutral to acidic monosaccharides decreased from 6.7 to 5.7 as the extraction temperature was increased from 30 to 90°C. Physiochemical and functional properties, including zeta-potential, surface morphology, emulsifying activity index (EAI) and emulsion stability index (ESI), water absorption capacity (WAC) and fat absorption capacity (FAC) of FG samples, were also investigated as a function of extraction temperature.
28243710	8	0	theme	conversion	1131:1140	arg1	FCR					1149:1151	FCR	1149:1151	FCR	1149:1151	However, zinc bacitracin reduced the feed conversion ratio (FCR) in treated birds, changed the composition and increased the diversity of their caecal microbiota by reducing dominant species.
28243710	8	0	theme	conversion	1131:1140	arg1	ratio					1142:1146	the feed conversion ratio	1122:1146	the feed conversion ratio (FCR) in treated birds	1122:1169	However, zinc bacitracin reduced the feed conversion ratio (FCR) in treated birds, changed the composition and increased the diversity of their caecal microbiota by reducing dominant species.
28243710	6	1	theme	10-day	856:861	arg1	period					863:868	a 10-day period	854:868	a 10-day period	854:868	Chicken growth performance was evaluated over a 10-day period, and caecal microbiota was characterised by sequencing of bacterial 16S rRNA gene amplicons.
28243710	2	2	theme	avilamycin	393:402	arg1	effect					363:368	the effect	359:368	the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens	359:458	Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and analysed associations between individual bacteria and growth performance.
28243710	4	3	theme	zinc	667:670	arg1	n = 48					709:714	n = 48	709:714	n = 48	709:714	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	4	3	theme	zinc	667:670	arg1	group					702:706	a zinc bacitracin (50 ppm) treatment group	665:706	a zinc bacitracin (50 ppm) treatment group (n = 48)	665:715	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	1	4	from	microbiota	305:314	arg1	production					326:335	animal production	319:335	animal production	319:335	Unravelling the mechanisms of how antibiotics influence growth performance through changes in gut microbiota can lead to the identification of highly productive microbiota in animal production.
28243710	2	5	from	effect	363:368	arg1	performance					414:424	growth performance	407:424	growth performance	407:424	Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and analysed associations between individual bacteria and growth performance.
28243710	2	5	from	effect	363:368	arg1	microbiota					437:446	caecal microbiota	430:446	caecal microbiota	430:446	Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and analysed associations between individual bacteria and growth performance.
28243710	3	6	theme	Cobb	609:612	arg1	broilers					614:621	96 individually caged 15-day-old Cobb broilers	576:621	96 individually caged 15-day-old Cobb broilers	576:621	Two trials were undertaken; each used 96 individually caged 15-day-old Cobb broilers.
28243710	8	7	theme	treated	1157:1163	arg1	birds					1165:1169	treated birds	1157:1169	treated birds	1157:1169	However, zinc bacitracin reduced the feed conversion ratio (FCR) in treated birds, changed the composition and increased the diversity of their caecal microbiota by reducing dominant species.
28243710	1	8	from	identification	269:282	arg1	production					326:335	animal production	319:335	animal production	319:335	Unravelling the mechanisms of how antibiotics influence growth performance through changes in gut microbiota can lead to the identification of highly productive microbiota in animal production.
28243710	3	9	theme	caged	592:596	arg1	broilers					614:621	96 individually caged 15-day-old Cobb broilers	576:621	96 individually caged 15-day-old Cobb broilers	576:621	Two trials were undertaken; each used 96 individually caged 15-day-old Cobb broilers.
28243710	8	10	theme	caecal	1233:1238	arg1	microbiota					1240:1249	their caecal microbiota	1227:1249	their caecal microbiota	1227:1249	However, zinc bacitracin reduced the feed conversion ratio (FCR) in treated birds, changed the composition and increased the diversity of their caecal microbiota by reducing dominant species.
28243710	9	11	theme	Lactobacillus	1451:1463	arg1	species					1465:1471	Lactobacillus species	1451:1471	Lactobacillus species	1451:1471	Avilamycin only produced minor reductions in the abundance of two microbial taxa, whereas zinc bacitracin produced relatively large shifts in a number of taxa, primarily Lactobacillus species.
28243710	6	12	theme	caecal	875:880	arg1	microbiota					882:891	caecal microbiota	875:891	caecal microbiota	875:891	Chicken growth performance was evaluated over a 10-day period, and caecal microbiota was characterised by sequencing of bacterial 16S rRNA gene amplicons.
28243710	8	13	theme	dominant	1263:1270	arg1	species					1272:1278	dominant species	1263:1278	dominant species	1263:1278	However, zinc bacitracin reduced the feed conversion ratio (FCR) in treated birds, changed the composition and increased the diversity of their caecal microbiota by reducing dominant species.
28243710	2	14	theme	growth	518:523	arg1	performance					525:535	growth performance	518:535	growth performance	518:535	Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and analysed associations between individual bacteria and growth performance.
28243710	0	15	from	mechanisms	18:27	arg1	production					73:82	animal production	66:82	animal production	66:82	Understanding the mechanisms of zinc bacitracin and avilamycin on animal production: linking gut microbiota and growth performance in chickens.
28243710	6	16	theme	gene	947:950	arg1	amplicons					952:960	bacterial 16S rRNA gene amplicons	928:960	bacterial 16S rRNA gene amplicons	928:960	Chicken growth performance was evaluated over a 10-day period, and caecal microbiota was characterised by sequencing of bacterial 16S rRNA gene amplicons.
28243710	6	17	theme	16S	938:940	arg1	amplicons					952:960	bacterial 16S rRNA gene amplicons	928:960	bacterial 16S rRNA gene amplicons	928:960	Chicken growth performance was evaluated over a 10-day period, and caecal microbiota was characterised by sequencing of bacterial 16S rRNA gene amplicons.
28243710	5	18	contain	had	726:728	arg2	n = 48					747:752	n = 48	747:752	n = 48	747:752	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	5	18	contain	had	726:728	arg2	group					792:796	an avilamycin (15 ppm) treatment group	759:796	an avilamycin (15 ppm) treatment group (n = 48)	759:805	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	5	18	contain	had	726:728	arg1	Trial					718:722	Trial 2	718:724	Trial 2	718:724	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	5	18	contain	had	726:728	arg2	group					740:744	a control group	730:744	a control group (n = 48)	730:753	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	5	18	contain	had	726:728	arg2	n = 48					799:804	n = 48	799:804	n = 48	799:804	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	2	19	theme	growth	407:412	arg1	performance					414:424	growth performance	407:424	growth performance	407:424	Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and analysed associations between individual bacteria and growth performance.
28243710	0	20	theme	animal	66:71	arg1	production					73:82	animal production	66:82	animal production	66:82	Understanding the mechanisms of zinc bacitracin and avilamycin on animal production: linking gut microbiota and growth performance in chickens.
28243710	10	21	theme	contrasting	1612:1622	arg1	effects					1624:1630	contrasting effects	1612:1630	contrasting effects of Lactobacillus spp	1612:1651	Also, a number of phylotypes closely related to lactobacilli species were positively or negatively correlated with FCR values, suggesting contrasting effects of Lactobacillus spp.
28243710	5	22	theme	15 ppm	774:779	arg1	n = 48					799:804	n = 48	799:804	n = 48	799:804	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	5	22	theme	15 ppm	774:779	arg1	group					792:796	an avilamycin (15 ppm) treatment group	759:796	an avilamycin (15 ppm) treatment group (n = 48)	759:805	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	5	23	theme	treatment	782:790	arg1	n = 48					799:804	n = 48	799:804	n = 48	799:804	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	5	23	theme	treatment	782:790	arg1	group					792:796	an avilamycin (15 ppm) treatment group	759:796	an avilamycin (15 ppm) treatment group (n = 48)	759:805	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	1	24	theme	productive	294:303	arg1	microbiota					305:314	highly productive microbiota	287:314	highly productive microbiota in animal production	287:335	Unravelling the mechanisms of how antibiotics influence growth performance through changes in gut microbiota can lead to the identification of highly productive microbiota in animal production.
28243710	10	25	theme	spp	1649:1651	arg1	effects					1624:1630	contrasting effects	1612:1630	contrasting effects of Lactobacillus spp	1612:1651	Also, a number of phylotypes closely related to lactobacilli species were positively or negatively correlated with FCR values, suggesting contrasting effects of Lactobacillus spp.
28243710	0	26	theme	zinc	32:35	arg1	bacitracin					37:46	zinc bacitracin	32:46	zinc bacitracin	32:46	Understanding the mechanisms of zinc bacitracin and avilamycin on animal production: linking gut microbiota and growth performance in chickens.
28243710	6	27	theme	Chicken	808:814	arg1	performance					823:833	Chicken growth performance	808:833	Chicken growth performance	808:833	Chicken growth performance was evaluated over a 10-day period, and caecal microbiota was characterised by sequencing of bacterial 16S rRNA gene amplicons.
28243710	11	28	theme	growth	1665:1670	arg1	performance					1672:1682	chicken growth performance	1657:1682	chicken growth performance	1657:1682	on chicken growth performance.
28243710	5	29	theme	avilamycin	762:771	arg1	n = 48					799:804	n = 48	799:804	n = 48	799:804	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	5	29	theme	avilamycin	762:771	arg1	group					792:796	an avilamycin (15 ppm) treatment group	759:796	an avilamycin (15 ppm) treatment group (n = 48)	759:805	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	7	30	theme	microbiota	1067:1076	arg1	structure					1078:1086	the microbiota structure	1063:1086	the microbiota structure	1063:1086	Avilamycin produced no effect on growth performance and exhibited little significant disturbance of the microbiota structure.
28243710	9	31	theme	microbial	1347:1355	arg1	taxa					1357:1360	two microbial taxa	1343:1360	two microbial taxa	1343:1360	Avilamycin only produced minor reductions in the abundance of two microbial taxa, whereas zinc bacitracin produced relatively large shifts in a number of taxa, primarily Lactobacillus species.
28243710	10	32	theme	related	1511:1517	arg1	phylotypes					1492:1501	phylotypes	1492:1501	phylotypes closely related to lactobacilli species	1492:1541	Also, a number of phylotypes closely related to lactobacilli species were positively or negatively correlated with FCR values, suggesting contrasting effects of Lactobacillus spp.
28243710	2	33	theme	bacitracin	378:387	arg1	effect					363:368	the effect	359:368	the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens	359:458	Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and analysed associations between individual bacteria and growth performance.
28243710	8	34	theme	zinc	1098:1101	arg1	bacitracin					1103:1112	zinc bacitracin	1098:1112	zinc bacitracin	1098:1112	However, zinc bacitracin reduced the feed conversion ratio (FCR) in treated birds, changed the composition and increased the diversity of their caecal microbiota by reducing dominant species.
28243710	2	35	from	microbiota	437:446	arg1	chickens					451:458	chickens	451:458	chickens	451:458	Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and analysed associations between individual bacteria and growth performance.
28243710	4	36	theme	control	638:644	arg1	n = 48					653:658	n = 48	653:658	n = 48	653:658	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	4	36	theme	control	638:644	arg1	group					646:650	a control group	636:650	a control group (n = 48)	636:659	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	10	37	theme	phylotypes	1492:1501	arg1	number					1482:1487	a number	1480:1487	a number of phylotypes closely related to lactobacilli species	1480:1541	Also, a number of phylotypes closely related to lactobacilli species were positively or negatively correlated with FCR values, suggesting contrasting effects of Lactobacillus spp.
28243710	1	38	from	production	326:335	arg1	identification					269:282	the identification	265:282	the identification of highly productive microbiota in animal production	265:335	Unravelling the mechanisms of how antibiotics influence growth performance through changes in gut microbiota can lead to the identification of highly productive microbiota in animal production.
28243710	9	39	theme	zinc	1371:1374	arg1	bacitracin					1376:1385	zinc bacitracin	1371:1385	zinc bacitracin	1371:1385	Avilamycin only produced minor reductions in the abundance of two microbial taxa, whereas zinc bacitracin produced relatively large shifts in a number of taxa, primarily Lactobacillus species.
28243710	12	40	from	strategies	1762:1771	arg1	poultry					1776:1782	poultry	1776:1782	poultry	1776:1782	By harnessing such bacteria, it may be possible to develop high-productivity strategies in poultry that rely on the use of probiotics and less on in-feed antibiotics.
28243710	7	41	theme	structure	1078:1086	arg1	disturbance					1048:1058	little significant disturbance	1029:1058	little significant disturbance of the microbiota structure	1029:1086	Avilamycin produced no effect on growth performance and exhibited little significant disturbance of the microbiota structure.
28243710	8	42	theme	feed	1126:1129	arg1	FCR					1149:1151	FCR	1149:1151	FCR	1149:1151	However, zinc bacitracin reduced the feed conversion ratio (FCR) in treated birds, changed the composition and increased the diversity of their caecal microbiota by reducing dominant species.
28243710	8	42	theme	feed	1126:1129	arg1	ratio					1142:1146	the feed conversion ratio	1122:1146	the feed conversion ratio (FCR) in treated birds	1122:1169	However, zinc bacitracin reduced the feed conversion ratio (FCR) in treated birds, changed the composition and increased the diversity of their caecal microbiota by reducing dominant species.
28243710	12	43	theme	high-productivity	1744:1760	arg1	strategies					1762:1771	high-productivity strategies	1744:1771	high-productivity strategies in poultry that rely on the use of probiotics and less on in-feed antibiotics	1744:1849	By harnessing such bacteria, it may be possible to develop high-productivity strategies in poultry that rely on the use of probiotics and less on in-feed antibiotics.
28243710	10	44	theme	lactobacilli	1522:1533	arg1	species					1535:1541	lactobacilli species	1522:1541	lactobacilli species	1522:1541	Also, a number of phylotypes closely related to lactobacilli species were positively or negatively correlated with FCR values, suggesting contrasting effects of Lactobacillus spp.
28243710	9	45	theme	large	1407:1411	arg1	shifts					1413:1418	relatively large shifts	1396:1418	relatively large shifts	1396:1418	Avilamycin only produced minor reductions in the abundance of two microbial taxa, whereas zinc bacitracin produced relatively large shifts in a number of taxa, primarily Lactobacillus species.
28243710	3	46	theme	15-day-old	598:607	arg1	broilers					614:621	96 individually caged 15-day-old Cobb broilers	576:621	96 individually caged 15-day-old Cobb broilers	576:621	Two trials were undertaken; each used 96 individually caged 15-day-old Cobb broilers.
28243710	6	47	theme	bacterial	928:936	arg1	amplicons					952:960	bacterial 16S rRNA gene amplicons	928:960	bacterial 16S rRNA gene amplicons	928:960	Chicken growth performance was evaluated over a 10-day period, and caecal microbiota was characterised by sequencing of bacterial 16S rRNA gene amplicons.
28243710	3	48	used	used	571:574	arg2	each					566:569	each	566:569	each	566:569	Two trials were undertaken; each used 96 individually caged 15-day-old Cobb broilers.
28243710	7	49	theme	little	1029:1034	arg1	disturbance					1048:1058	little significant disturbance	1029:1058	little significant disturbance of the microbiota structure	1029:1086	Avilamycin produced no effect on growth performance and exhibited little significant disturbance of the microbiota structure.
28243710	1	50	theme	growth	200:205	arg1	performance					207:217	growth performance	200:217	growth performance	200:217	Unravelling the mechanisms of how antibiotics influence growth performance through changes in gut microbiota can lead to the identification of highly productive microbiota in animal production.
28243710	4	51	theme	bacitracin	672:681	arg1	n = 48					709:714	n = 48	709:714	n = 48	709:714	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	4	51	theme	bacitracin	672:681	arg1	group					702:706	a zinc bacitracin (50 ppm) treatment group	665:706	a zinc bacitracin (50 ppm) treatment group (n = 48)	665:715	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	9	52	theme	taxa	1435:1438	arg1	number					1425:1430	a number	1423:1430	a number of taxa	1423:1438	Avilamycin only produced minor reductions in the abundance of two microbial taxa, whereas zinc bacitracin produced relatively large shifts in a number of taxa, primarily Lactobacillus species.
28243710	10	53	theme	FCR	1589:1591	arg1	values					1593:1598	FCR values	1589:1598	FCR values	1589:1598	Also, a number of phylotypes closely related to lactobacilli species were positively or negatively correlated with FCR values, suggesting contrasting effects of Lactobacillus spp.
28243710	7	54	theme	growth	996:1001	arg1	performance					1003:1013	growth performance	996:1013	growth performance	996:1013	Avilamycin produced no effect on growth performance and exhibited little significant disturbance of the microbiota structure.
28243710	1	55	from	changes	227:233	arg1	microbiota					242:251	gut microbiota	238:251	gut microbiota	238:251	Unravelling the mechanisms of how antibiotics influence growth performance through changes in gut microbiota can lead to the identification of highly productive microbiota in animal production.
28243710	8	56	theme	microbiota	1240:1249	arg1	diversity					1214:1222	the diversity	1210:1222	the diversity of their caecal microbiota	1210:1249	However, zinc bacitracin reduced the feed conversion ratio (FCR) in treated birds, changed the composition and increased the diversity of their caecal microbiota by reducing dominant species.
28243710	5	57	theme	control	732:738	arg1	group					740:744	a control group	730:744	a control group (n = 48)	730:753	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	5	57	theme	control	732:738	arg1	n = 48					747:752	n = 48	747:752	n = 48	747:752	Trial 2 had a control group (n = 48) and an avilamycin (15 ppm) treatment group (n = 48).
28243710	4	58	contain	had	632:634	arg1	Trial					624:628	Trial 1	624:630	Trial 1	624:630	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	4	58	contain	had	632:634	arg2	group					646:650	a control group	636:650	a control group (n = 48)	636:659	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	4	58	contain	had	632:634	arg2	n = 48					709:714	n = 48	709:714	n = 48	709:714	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	4	58	contain	had	632:634	arg2	group					702:706	a zinc bacitracin (50 ppm) treatment group	665:706	a zinc bacitracin (50 ppm) treatment group (n = 48)	665:715	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	4	58	contain	had	632:634	arg2	n = 48					653:658	n = 48	653:658	n = 48	653:658	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	12	59	theme	such	1699:1702	arg1	bacteria					1704:1711	such bacteria	1699:1711	such bacteria	1699:1711	By harnessing such bacteria, it may be possible to develop high-productivity strategies in poultry that rely on the use of probiotics and less on in-feed antibiotics.
28243710	4	60	theme	50 ppm	684:689	arg1	n = 48					709:714	n = 48	709:714	n = 48	709:714	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	4	60	theme	50 ppm	684:689	arg1	group					702:706	a zinc bacitracin (50 ppm) treatment group	665:706	a zinc bacitracin (50 ppm) treatment group (n = 48)	665:715	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	6	61	theme	amplicons	952:960	arg1	sequencing					914:923	sequencing	914:923	sequencing of bacterial 16S rRNA gene amplicons	914:960	Chicken growth performance was evaluated over a 10-day period, and caecal microbiota was characterised by sequencing of bacterial 16S rRNA gene amplicons.
28243710	7	62	theme	significant	1036:1046	arg1	disturbance					1048:1058	little significant disturbance	1029:1058	little significant disturbance of the microbiota structure	1029:1086	Avilamycin produced no effect on growth performance and exhibited little significant disturbance of the microbiota structure.
28243710	4	63	theme	treatment	692:700	arg1	n = 48					709:714	n = 48	709:714	n = 48	709:714	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	4	63	theme	treatment	692:700	arg1	group					702:706	a zinc bacitracin (50 ppm) treatment group	665:706	a zinc bacitracin (50 ppm) treatment group (n = 48)	665:715	Trial 1 had a control group (n = 48) and a zinc bacitracin (50 ppm) treatment group (n = 48).
28243710	0	64	theme	gut	93:95	arg1	microbiota					97:106	gut microbiota	93:106	gut microbiota	93:106	Understanding the mechanisms of zinc bacitracin and avilamycin on animal production: linking gut microbiota and growth performance in chickens.
28243710	6	65	theme	rRNA	942:945	arg1	amplicons					952:960	bacterial 16S rRNA gene amplicons	928:960	bacterial 16S rRNA gene amplicons	928:960	Chicken growth performance was evaluated over a 10-day period, and caecal microbiota was characterised by sequencing of bacterial 16S rRNA gene amplicons.
28243710	9	66	from	reductions	1312:1321	arg1	abundance					1330:1338	the abundance	1326:1338	the abundance of two microbial taxa	1326:1360	Avilamycin only produced minor reductions in the abundance of two microbial taxa, whereas zinc bacitracin produced relatively large shifts in a number of taxa, primarily Lactobacillus species.
28243710	1	67	theme	gut	238:240	arg1	microbiota					242:251	gut microbiota	238:251	gut microbiota	238:251	Unravelling the mechanisms of how antibiotics influence growth performance through changes in gut microbiota can lead to the identification of highly productive microbiota in animal production.
28243710	9	68	theme	minor	1306:1310	arg1	reductions					1312:1321	minor reductions	1306:1321	minor reductions in the abundance of two microbial taxa	1306:1360	Avilamycin only produced minor reductions in the abundance of two microbial taxa, whereas zinc bacitracin produced relatively large shifts in a number of taxa, primarily Lactobacillus species.
28243710	12	69	theme	in-feed	1831:1837	arg1	antibiotics					1839:1849	in-feed antibiotics	1831:1849	in-feed antibiotics	1831:1849	By harnessing such bacteria, it may be possible to develop high-productivity strategies in poultry that rely on the use of probiotics and less on in-feed antibiotics.
28243710	2	70	theme	caecal	430:435	arg1	microbiota					437:446	caecal microbiota	430:446	caecal microbiota	430:446	Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and analysed associations between individual bacteria and growth performance.
28243710	0	71	theme	bacitracin	37:46	arg1	mechanisms					18:27	the mechanisms	14:27	the mechanisms of zinc bacitracin and avilamycin on animal production	14:82	Understanding the mechanisms of zinc bacitracin and avilamycin on animal production: linking gut microbiota and growth performance in chickens.
28243710	1	72	theme	microbiota	305:314	arg1	identification					269:282	the identification	265:282	the identification of highly productive microbiota in animal production	265:335	Unravelling the mechanisms of how antibiotics influence growth performance through changes in gut microbiota can lead to the identification of highly productive microbiota in animal production.
28243710	6	73	theme	growth	816:821	arg1	performance					823:833	Chicken growth performance	808:833	Chicken growth performance	808:833	Chicken growth performance was evaluated over a 10-day period, and caecal microbiota was characterised by sequencing of bacterial 16S rRNA gene amplicons.
28243710	2	74	theme	individual	494:503	arg1	bacteria					505:512	individual bacteria	494:512	individual bacteria	494:512	Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and analysed associations between individual bacteria and growth performance.
28243710	11	75	theme	chicken	1657:1663	arg1	performance					1672:1682	chicken growth performance	1657:1682	chicken growth performance	1657:1682	on chicken growth performance.
28243710	0	76	theme	avilamycin	52:61	arg1	mechanisms					18:27	the mechanisms	14:27	the mechanisms of zinc bacitracin and avilamycin on animal production	14:82	Understanding the mechanisms of zinc bacitracin and avilamycin on animal production: linking gut microbiota and growth performance in chickens.
28243710	1	77	theme	animal	319:324	arg1	production					326:335	animal production	319:335	animal production	319:335	Unravelling the mechanisms of how antibiotics influence growth performance through changes in gut microbiota can lead to the identification of highly productive microbiota in animal production.
28243710	12	78	theme	probiotics	1808:1817	arg1	use					1801:1803	the use	1797:1803	the use of probiotics and less	1797:1826	By harnessing such bacteria, it may be possible to develop high-productivity strategies in poultry that rely on the use of probiotics and less on in-feed antibiotics.
28243710	12	79	theme	less	1823:1826	arg1	use					1801:1803	the use	1797:1803	the use of probiotics and less	1797:1826	By harnessing such bacteria, it may be possible to develop high-productivity strategies in poultry that rely on the use of probiotics and less on in-feed antibiotics.
28243710	8	80	from	ratio	1142:1146	arg1	birds					1165:1169	treated birds	1157:1169	treated birds	1157:1169	However, zinc bacitracin reduced the feed conversion ratio (FCR) in treated birds, changed the composition and increased the diversity of their caecal microbiota by reducing dominant species.
28243710	7	81	from	effect	986:991	arg1	performance					1003:1013	growth performance	996:1013	growth performance	996:1013	Avilamycin produced no effect on growth performance and exhibited little significant disturbance of the microbiota structure.
28243710	9	82	theme	taxa	1357:1360	arg1	abundance					1330:1338	the abundance	1326:1338	the abundance of two microbial taxa	1326:1360	Avilamycin only produced minor reductions in the abundance of two microbial taxa, whereas zinc bacitracin produced relatively large shifts in a number of taxa, primarily Lactobacillus species.
28243710	2	83	from	performance	414:424	arg1	chickens					451:458	chickens	451:458	chickens	451:458	Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and analysed associations between individual bacteria and growth performance.
28243710	0	84	theme	growth	112:117	arg1	performance					119:129	growth performance	112:129	growth performance	112:129	Understanding the mechanisms of zinc bacitracin and avilamycin on animal production: linking gut microbiota and growth performance in chickens.
28243710	2	85	theme	zinc	373:376	arg1	bacitracin					378:387	zinc bacitracin	373:387	zinc bacitracin	373:387	Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and analysed associations between individual bacteria and growth performance.
26319633	5	0	theme	better	764:769	arg1	protection					771:780	better protection	764:780	better protection of loaded antigen till	764:803	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	3	1	theme	storage	499:505	arg1	studies					517:523	storage stability studies	499:523	storage stability studies	499:523	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	4	2	theme	size	592:595	arg1	minimum					573:579	minimum	573:579	minimum of average size of 5 μm (PDI, 0.184)	573:616	Microparticles with minimum of average size of 5 μm (PDI, 0.184) were chosen after optimizing the composition and process conditions.
26319633	8	3	theme	laser	1393:1397	arg1	microscopy					1408:1417	confocal laser scanning microscopy	1384:1417	confocal laser scanning microscopy	1384:1417	The developed ACMs were able to transport the antigen effectively to the M cell as revealed by confocal laser scanning microscopy.
26319633	3	4	theme	stability	507:515	arg1	studies					517:523	storage stability studies	499:523	storage stability studies	499:523	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	7	5	theme	zeta	1228:1231	arg1	potential					1233:1241	the zeta potential	1224:1241	the zeta potential	1224:1241	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	4	6	theme	average	584:590	arg1	size					592:595	average size	584:595	average size of 5 μm (PDI, 0.184)	584:616	Microparticles with minimum of average size of 5 μm (PDI, 0.184) were chosen after optimizing the composition and process conditions.
26319633	5	7	dep	cells	838:842	arg1	i.e.					831:834	i.e.	831:834	i.e.	831:834	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	7	8	theme	predominance	1260:1271	arg1	virtue					1246:1251	virtue	1246:1251	virtue of its predominance on the surface	1246:1286	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	8	9	theme	confocal	1384:1391	arg1	microscopy					1408:1417	confocal laser scanning microscopy	1384:1417	confocal laser scanning microscopy	1384:1417	The developed ACMs were able to transport the antigen effectively to the M cell as revealed by confocal laser scanning microscopy.
26319633	8	10	theme	M	1362:1362	arg1	cell					1364:1367	the M cell	1358:1367	the M cell	1358:1367	The developed ACMs were able to transport the antigen effectively to the M cell as revealed by confocal laser scanning microscopy.
26319633	7	11	theme	alginate	1164:1171	arg1	coating					1153:1159	the coating	1149:1159	the coating of alginate on chitosan microparticles	1149:1198	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	9	12	theme	DTx-loaded	1429:1438	arg1	ACMs					1440:1443	DTx-loaded ACMs	1429:1443	DTx-loaded ACMs	1429:1443	Further, DTx-loaded ACMs demonstrated significant immune responses at serum IgG as well as mucosal sIgA level.
26319633	7	13	theme	zeta	1075:1078	arg1	potential					1080:1088	zeta potential	1075:1088	zeta potential	1075:1088	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	6	14	theme	±	1036:1036	arg1	mV					1042:1043	-32.6 ± 4.2 mV	1030:1043	-32.6 ± 4.2 mV	1030:1043	The zeta-potential values for uncoated chitosan microparticles and ACMs were found to be +29 ± 3.3 mV and -32.6 ± 4.2 mV, respectively.
26319633	3	15	theme	stability	480:488	arg1	studies					490:496	process stability studies	472:496	process stability studies	472:496	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	16	theme	developed	340:348	arg1	system					350:355	The developed system	336:355	The developed system	336:355	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	8	17	theme	scanning	1399:1406	arg1	microscopy					1408:1417	confocal laser scanning microscopy	1384:1417	confocal laser scanning microscopy	1384:1417	The developed ACMs were able to transport the antigen effectively to the M cell as revealed by confocal laser scanning microscopy.
26319633	3	18	theme	in-vivo	529:535	arg1	studies					544:550	in-vivo uptake studies	529:550	in-vivo uptake studies	529:550	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	7	19	from	virtue	1246:1251	arg1	surface					1280:1286	the surface	1276:1286	the surface	1276:1286	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	3	20	theme	acidic	430:435	arg1	size					388:391	particle size	379:391	particle size	379:391	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	20	theme	acidic	430:435	arg1	studies					460:466	acidic degradation protection studies	430:466	acidic degradation protection studies	430:466	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	5	21	theme	alginate	743:750	arg1	coating					752:758	alginate coating	743:758	alginate coating for better protection of loaded antigen till	743:803	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	2	22	theme	current	156:162	arg1	study					164:168	The current study	152:168	The current study	152:168	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	6	23	theme	chitosan	963:970	arg1	microparticles					972:985	uncoated chitosan microparticles	954:985	uncoated chitosan microparticles	954:985	The zeta-potential values for uncoated chitosan microparticles and ACMs were found to be +29 ± 3.3 mV and -32.6 ± 4.2 mV, respectively.
26319633	2	24	theme	Diphtheria	270:279	arg1	DTx					289:291	DTx	289:291	DTx	289:291	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	2	24	theme	Diphtheria	270:279	arg1	toxoid					281:286	Diphtheria toxoid	270:286	Diphtheria toxoid (DTx) employing formulation by design approach	270:333	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	3	25	theme	degradation	437:447	arg1	size					388:391	particle size	379:391	particle size	379:391	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	25	theme	degradation	437:447	arg1	studies					460:466	acidic degradation protection studies	430:466	acidic degradation protection studies	430:466	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	0	26	theme	coated	9:14	arg1	microparticles					25:38	Alginate coated chitosan microparticles	0:38	Alginate coated chitosan microparticles	0:38	Alginate coated chitosan microparticles mediated oral delivery of diphtheria toxoid.
26319633	5	27	theme	amount	897:902	arg1	antigen					904:910	higher amount antigen	890:910	higher amount antigen	890:910	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	7	28	from	coating	1153:1159	arg1	microparticles					1185:1198	chitosan microparticles	1176:1198	chitosan microparticles	1176:1198	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	4	29	theme	composition	651:661	arg1	conditions					675:684	the composition and process conditions	647:684	the composition and process conditions	647:684	Microparticles with minimum of average size of 5 μm (PDI, 0.184) were chosen after optimizing the composition and process conditions.
26319633	0	30	theme	Alginate	0:7	arg1	microparticles					25:38	Alginate coated chitosan microparticles	0:38	Alginate coated chitosan microparticles	0:38	Alginate coated chitosan microparticles mediated oral delivery of diphtheria toxoid.
26319633	6	31	theme	zeta-potential	928:941	arg1	values					943:948	The zeta-potential values	924:948	The zeta-potential values for uncoated chitosan microparticles and ACMs	924:994	The zeta-potential values for uncoated chitosan microparticles and ACMs were found to be +29 ± 3.3 mV and -32.6 ± 4.2 mV, respectively.
26319633	6	31	theme	zeta-potential	928:941	arg1	mV					1023:1024	+29 ± 3.3 mV	1013:1024	+29 ± 3.3 mV	1013:1024	The zeta-potential values for uncoated chitosan microparticles and ACMs were found to be +29 ± 3.3 mV and -32.6 ± 4.2 mV, respectively.
26319633	9	32	theme	mucosal	1511:1517	arg1	level					1524:1528	mucosal sIgA level	1511:1528	mucosal sIgA level	1511:1528	Further, DTx-loaded ACMs demonstrated significant immune responses at serum IgG as well as mucosal sIgA level.
26319633	5	33	theme	antigen	904:910	arg1	PPs					868:870	PPs	868:870	PPs	868:870	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	5	33	theme	antigen	904:910	arg1	transport					877:885	transport	877:885	transport of higher amount antigen to the PPs	877:921	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	5	33	theme	antigen	904:910	arg1	patches					859:865	the Peyer's patches	847:865	the Peyer's patches (PPs)	847:871	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	7	34	from	predominance	1260:1271	arg1	surface					1280:1286	the surface	1276:1286	the surface	1276:1286	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	6	35	theme	±	1017:1017	arg1	values					943:948	The zeta-potential values	924:948	The zeta-potential values for uncoated chitosan microparticles and ACMs	924:994	The zeta-potential values for uncoated chitosan microparticles and ACMs were found to be +29 ± 3.3 mV and -32.6 ± 4.2 mV, respectively.
26319633	6	35	theme	±	1017:1017	arg1	mV					1023:1024	+29 ± 3.3 mV	1013:1024	+29 ± 3.3 mV	1013:1024	The zeta-potential values for uncoated chitosan microparticles and ACMs were found to be +29 ± 3.3 mV and -32.6 ± 4.2 mV, respectively.
26319633	5	36	theme	higher	890:895	arg1	amount					897:902	higher amount	890:902	higher amount antigen	890:910	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	5	37	theme	antigen	792:798	arg1	till					800:803	loaded antigen till	785:803	loaded antigen till	785:803	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	6	38	theme	+29	1013:1015	arg1	values					943:948	The zeta-potential values	924:948	The zeta-potential values for uncoated chitosan microparticles and ACMs	924:994	The zeta-potential values for uncoated chitosan microparticles and ACMs were found to be +29 ± 3.3 mV and -32.6 ± 4.2 mV, respectively.
26319633	6	38	theme	+29	1013:1015	arg1	mV					1023:1024	+29 ± 3.3 mV	1013:1024	+29 ± 3.3 mV	1013:1024	The zeta-potential values for uncoated chitosan microparticles and ACMs were found to be +29 ± 3.3 mV and -32.6 ± 4.2 mV, respectively.
26319633	3	39	theme	particle	379:386	arg1	size					388:391	particle size	379:391	particle size	379:391	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	39	theme	particle	379:386	arg1	studies					460:466	acidic degradation protection studies	430:466	acidic degradation protection studies	430:466	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	39	theme	particle	379:386	arg1	morphology					418:427	surface morphology	410:427	surface morphology	410:427	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	39	theme	particle	379:386	arg1	potential					399:407	zeta potential	394:407	zeta potential	394:407	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	5	40	theme	optimized	691:699	arg1	microparticles					710:723	The optimized chitosan microparticles	687:723	The optimized chitosan microparticles	687:723	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	7	41	theme	potential	1080:1088	arg1	change					1065:1070	This change	1060:1070	This change of zeta potential	1060:1088	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	4	42	theme	0.184	611:615	arg1	PDI					606:608	PDI	606:608	PDI	606:608	Microparticles with minimum of average size of 5 μm (PDI, 0.184) were chosen after optimizing the composition and process conditions.
26319633	0	43	theme	oral	49:52	arg1	delivery					54:61	oral delivery	49:61	oral delivery of diphtheria toxoid	49:82	Alginate coated chitosan microparticles mediated oral delivery of diphtheria toxoid.
26319633	9	44	theme	significant	1458:1468	arg1	responses					1477:1485	significant immune responses	1458:1485	significant immune responses	1458:1485	Further, DTx-loaded ACMs demonstrated significant immune responses at serum IgG as well as mucosal sIgA level.
26319633	5	45	theme	chitosan	701:708	arg1	microparticles					710:723	The optimized chitosan microparticles	687:723	The optimized chitosan microparticles	687:723	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	5	46	theme	M	836:836	arg1	cells					838:842	M cells	836:842	M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs	836:921	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	9	47	theme	serum	1490:1494	arg1	IgG					1496:1498	serum IgG	1490:1498	serum IgG	1490:1498	Further, DTx-loaded ACMs demonstrated significant immune responses at serum IgG as well as mucosal sIgA level.
26319633	7	48	from	surface	1280:1286	arg1	virtue					1246:1251	virtue	1246:1251	virtue of its predominance on the surface	1246:1286	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	3	49	theme	protection	449:458	arg1	size					388:391	particle size	379:391	particle size	379:391	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	49	theme	protection	449:458	arg1	studies					460:466	acidic degradation protection studies	430:466	acidic degradation protection studies	430:466	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	50	theme	surface	410:416	arg1	size					388:391	particle size	379:391	particle size	379:391	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	50	theme	surface	410:416	arg1	morphology					418:427	surface morphology	410:427	surface morphology	410:427	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	5	51	theme	loaded	785:790	arg1	till					800:803	loaded antigen till	785:803	loaded antigen till	785:803	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	6	52	theme	uncoated	954:961	arg1	microparticles					972:985	uncoated chitosan microparticles	954:985	uncoated chitosan microparticles	954:985	The zeta-potential values for uncoated chitosan microparticles and ACMs were found to be +29 ± 3.3 mV and -32.6 ± 4.2 mV, respectively.
26319633	3	53	theme	zeta	394:397	arg1	size					388:391	particle size	379:391	particle size	379:391	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	53	theme	zeta	394:397	arg1	potential					399:407	zeta potential	394:407	zeta potential	394:407	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	7	54	dep	coated	1107:1112	arg1	to					1104:1105	to	1104:1105	to	1104:1105	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	2	55	theme	chitosan	227:234	arg1	microparticles					236:249	"optimized" alginate coated chitosan microparticles	199:249	"optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach	199:333	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	2	55	theme	chitosan	227:234	arg1	ACMs					252:255	ACMs	252:255	ACMs	252:255	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	3	56	from	size	388:391	arg1	studies					544:550	in-vivo uptake studies	529:550	in-vivo uptake studies	529:550	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	56	from	size	388:391	arg1	studies					490:496	process stability studies	472:496	process stability studies	472:496	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	56	from	size	388:391	arg1	studies					517:523	storage stability studies	499:523	storage stability studies	499:523	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	7	57	theme	chitosan	1176:1183	arg1	microparticles					1185:1198	chitosan microparticles	1176:1198	chitosan microparticles	1176:1198	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	5	58	theme	till	800:803	arg1	protection					771:780	better protection	764:780	better protection of loaded antigen till	764:803	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	4	59	with	Microparticles	553:566	arg1	minimum					573:579	minimum	573:579	minimum of average size of 5 μm (PDI, 0.184)	573:616	Microparticles with minimum of average size of 5 μm (PDI, 0.184) were chosen after optimizing the composition and process conditions.
26319633	2	60	theme	alginate	211:218	arg1	microparticles					236:249	"optimized" alginate coated chitosan microparticles	199:249	"optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach	199:333	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	2	60	theme	alginate	211:218	arg1	ACMs					252:255	ACMs	252:255	ACMs	252:255	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	2	61	theme	coated	220:225	arg1	microparticles					236:249	"optimized" alginate coated chitosan microparticles	199:249	"optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach	199:333	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	2	61	theme	coated	220:225	arg1	ACMs					252:255	ACMs	252:255	ACMs	252:255	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	9	62	theme	immune	1470:1475	arg1	responses					1477:1485	significant immune responses	1458:1485	significant immune responses	1458:1485	Further, DTx-loaded ACMs demonstrated significant immune responses at serum IgG as well as mucosal sIgA level.
26319633	2	63	theme	"	209:209	arg1	microparticles					236:249	"optimized" alginate coated chitosan microparticles	199:249	"optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach	199:333	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	2	63	theme	"	209:209	arg1	ACMs					252:255	ACMs	252:255	ACMs	252:255	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	4	64	dep	composition	651:661	arg1	the					647:649	the	647:649	the	647:649	Microparticles with minimum of average size of 5 μm (PDI, 0.184) were chosen after optimizing the composition and process conditions.
26319633	3	65	theme	process	472:478	arg1	studies					490:496	process stability studies	472:496	process stability studies	472:496	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	3	66	theme	uptake	537:542	arg1	studies					544:550	in-vivo uptake studies	529:550	in-vivo uptake studies	529:550	The developed system was characterized for particle size, zeta potential, surface morphology, acidic degradation protection studies, in process stability studies, storage stability studies and in-vivo uptake studies.
26319633	2	67	theme	optimized	200:208	arg1	microparticles					236:249	"optimized" alginate coated chitosan microparticles	199:249	"optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach	199:333	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	2	67	theme	optimized	200:208	arg1	ACMs					252:255	ACMs	252:255	ACMs	252:255	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	5	68	theme	uptake	819:824	arg1	site					826:829	uptake site	819:829	uptake site	819:829	The optimized chitosan microparticles were subjected to alginate coating for better protection of loaded antigen till it reached to uptake site i.e. M cells in the Peyer's patches (PPs) and transport of higher amount antigen to the PPs.
26319633	2	69	theme	design	319:324	arg1	approach					326:333	design approach	319:333	design approach	319:333	The current study was embarked upon to develop "optimized" alginate coated chitosan microparticles (ACMs) loaded with Diphtheria toxoid (DTx) employing formulation by design approach.
26319633	4	70	theme	μm	602:603	arg1	size					592:595	average size	584:595	average size of 5 μm (PDI, 0.184)	584:616	Microparticles with minimum of average size of 5 μm (PDI, 0.184) were chosen after optimizing the composition and process conditions.
26319633	0	71	theme	toxoid	77:82	arg1	delivery					54:61	oral delivery	49:61	oral delivery of diphtheria toxoid	49:82	Alginate coated chitosan microparticles mediated oral delivery of diphtheria toxoid.
26319633	4	72	dep	μm	602:603	arg1	PDI					606:608	PDI	606:608	PDI	606:608	Microparticles with minimum of average size of 5 μm (PDI, 0.184) were chosen after optimizing the composition and process conditions.
26319633	9	73	theme	sIgA	1519:1522	arg1	level					1524:1528	mucosal sIgA level	1511:1528	mucosal sIgA level	1511:1528	Further, DTx-loaded ACMs demonstrated significant immune responses at serum IgG as well as mucosal sIgA level.
26319633	7	74	theme	negative	1207:1214	arg1	side					1216:1219	negative side	1207:1219	negative side of the zeta potential by virtue of its predominance on the surface	1207:1286	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	4	75	theme	process	667:673	arg1	conditions					675:684	the composition and process conditions	647:684	the composition and process conditions	647:684	Microparticles with minimum of average size of 5 μm (PDI, 0.184) were chosen after optimizing the composition and process conditions.
26319633	7	76	theme	potential	1233:1241	arg1	side					1216:1219	negative side	1207:1219	negative side of the zeta potential by virtue of its predominance on the surface	1207:1286	This change of zeta potential, for uncoated to coated, can be explained by the fact that the coating of alginate on chitosan microparticles led to negative side of the zeta potential by virtue of its predominance on the surface.
26319633	0	77	theme	diphtheria	66:75	arg1	toxoid					77:82	diphtheria toxoid	66:82	diphtheria toxoid	66:82	Alginate coated chitosan microparticles mediated oral delivery of diphtheria toxoid.
26319633	0	78	theme	chitosan	16:23	arg1	microparticles					25:38	Alginate coated chitosan microparticles	0:38	Alginate coated chitosan microparticles	0:38	Alginate coated chitosan microparticles mediated oral delivery of diphtheria toxoid.
26319633	8	79	theme	developed	1293:1301	arg1	able					1313:1316	able	1313:1316	able	1313:1316	The developed ACMs were able to transport the antigen effectively to the M cell as revealed by confocal laser scanning microscopy.
26319633	8	79	theme	developed	1293:1301	arg1	ACMs					1303:1306	The developed ACMs	1289:1306	The developed ACMs	1289:1306	The developed ACMs were able to transport the antigen effectively to the M cell as revealed by confocal laser scanning microscopy.
28024617	2	0	theme	pore	446:449	arg1	size					451:454	the pore size	442:454	the pore size	442:454	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	1	1	theme	eco-friendly	275:286	arg1	biocomposite					288:299	a novel eco-friendly biocomposite	267:299	a novel eco-friendly biocomposite	267:299	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	2	2	from	effect	432:437	arg1	size					451:454	the pore size	442:454	the pore size	442:454	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	2	2	from	effect	432:437	arg1	properties					496:505	mechanical and liquid uptake properties	467:505	mechanical and liquid uptake properties	467:505	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	2	2	from	effect	432:437	arg1	biodegradability					511:526	biodegradability	511:526	biodegradability	511:526	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	2	2	from	effect	432:437	arg1	porosity					457:464	porosity	457:464	porosity	457:464	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	10	3	theme	promising	1255:1263	arg1	biocomposite					1214:1225	the biocomposite	1210:1225	the biocomposite designed in this study	1210:1248	All these results suggest that the biocomposite designed in this study is a promising biomaterial for potential skin tissue engineering.
28024617	10	3	theme	promising	1255:1263	arg1	biomaterial					1265:1275	a promising biomaterial	1253:1275	a promising biomaterial for potential skin tissue engineering	1253:1313	All these results suggest that the biocomposite designed in this study is a promising biomaterial for potential skin tissue engineering.
28024617	4	4	theme	%	537:537	arg1	BBP					539:541	% BBP the biocomposite	537:558	% BBP the biocomposite	537:558	% BBP the biocomposite exhibited enhanced mechanical properties and biodegradability rate as compared to the pristine sample.
28024617	0	5	theme	alcohol	85:91	arg1	biocomposite					120:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
28024617	1	6	theme	bovine	221:226	arg1	BBP					241:243	BBP	241:243	BBP	241:243	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	1	6	theme	bovine	221:226	arg1	powder					233:238	bovine bone powder	221:238	bovine bone powder (BBP)	221:244	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	0	7	theme	bone	101:104	arg1	biocomposite					120:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
28024617	7	8	dep	ex	865:866	arg1	vivo					868:871	vivo	868:871	vivo	868:871	In vivo and ex vivo experiments revealed that ChS/PVA-BBP biocomposites are biocompatibility materials without exert pro-inflammatory responses.
28024617	7	9	theme	pro-inflammatory	970:985	arg1	responses					987:995	pro-inflammatory responses	970:995	pro-inflammatory responses	970:995	In vivo and ex vivo experiments revealed that ChS/PVA-BBP biocomposites are biocompatibility materials without exert pro-inflammatory responses.
28024617	1	10	theme	bone	228:231	arg1	BBP					241:243	BBP	241:243	BBP	241:243	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	1	10	theme	bone	228:231	arg1	powder					233:238	bovine bone powder	221:238	bovine bone powder (BBP)	221:244	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	0	11	theme	/bovine	93:99	arg1	biocomposite					120:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
28024617	1	12	theme	cyclic	309:314	arg1	freeze-thawing					316:329	cyclic freeze-thawing	309:329	cyclic freeze-thawing	309:329	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	9	13	theme	low-cost	1110:1117	arg1	insurance					1168:1176	insurance	1168:1176	insurance	1168:1176	Taken together, BBP is a low-cost source of hydroxyapatite and collagen, which are insurance.
28024617	9	13	theme	low-cost	1110:1117	arg1	BBP					1101:1103	BBP	1101:1103	BBP	1101:1103	Taken together, BBP is a low-cost source of hydroxyapatite and collagen, which are insurance.
28024617	9	13	theme	low-cost	1110:1117	arg1	source					1119:1124	a low-cost source	1108:1124	a low-cost source	1108:1124	Taken together, BBP is a low-cost source of hydroxyapatite and collagen, which are insurance.
28024617	10	14	theme	tissue	1296:1301	arg1	engineering					1303:1313	potential skin tissue engineering	1281:1313	potential skin tissue engineering	1281:1313	All these results suggest that the biocomposite designed in this study is a promising biomaterial for potential skin tissue engineering.
28024617	0	15	theme	porous	113:118	arg1	biocomposite					120:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
28024617	2	16	contain	has	415:417	arg1	content					400:406	the content	396:406	the content of BBP	396:413	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	2	16	contain	has	415:417	arg2	effect					432:437	a remarkable effect	419:437	a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability	419:526	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	10	17	theme	skin	1291:1294	arg1	engineering					1303:1313	potential skin tissue engineering	1281:1313	potential skin tissue engineering	1281:1313	All these results suggest that the biocomposite designed in this study is a promising biomaterial for potential skin tissue engineering.
28024617	2	18	theme	systematic	358:367	arg1	investigation					369:381	The systematic investigation	354:381	The systematic investigation	354:381	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	0	19	theme	powder	106:111	arg1	biocomposite					120:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
28024617	7	20	theme	ChS/PVA-BBP	899:909	arg1	biocomposites					911:923	ChS/PVA-BBP biocomposites	899:923	ChS/PVA-BBP biocomposites	899:923	In vivo and ex vivo experiments revealed that ChS/PVA-BBP biocomposites are biocompatibility materials without exert pro-inflammatory responses.
28024617	7	20	theme	ChS/PVA-BBP	899:909	arg1	materials					946:954	biocompatibility materials	929:954	biocompatibility materials	929:954	In vivo and ex vivo experiments revealed that ChS/PVA-BBP biocomposites are biocompatibility materials without exert pro-inflammatory responses.
28024617	10	21	theme	potential	1281:1289	arg1	engineering					1303:1313	potential skin tissue engineering	1281:1313	potential skin tissue engineering	1281:1313	All these results suggest that the biocomposite designed in this study is a promising biomaterial for potential skin tissue engineering.
28024617	1	22	theme	sulfated	163:170	arg1	glycosaminoglycan					172:188	a sulfated glycosaminoglycan	161:188	a sulfated glycosaminoglycan	161:188	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	1	22	theme	sulfated	163:170	arg1	sulfate					146:152	Chondroitin sulfate	134:152	Chondroitin sulfate (ChS)	134:158	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	1	23	theme	mild	337:340	arg1	conditions					342:351	mild conditions	337:351	mild conditions	337:351	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	2	24	theme	BBP	411:413	arg1	content					400:406	the content	396:406	the content of BBP	396:413	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	8	25	theme	treatment	1074:1082	arg1	15days					1064:1069	15days	1064:1069	15days of treatment	1064:1082	The biocomposite was completely biodegraded and bioresorbed after 15days of treatment.
28024617	7	26	dep	In	853:854	arg1	vivo					856:859	vivo	856:859	vivo	856:859	In vivo and ex vivo experiments revealed that ChS/PVA-BBP biocomposites are biocompatibility materials without exert pro-inflammatory responses.
28024617	6	27	theme	In	762:763	arg1	assays					771:776	In vitro assays	762:776	In vitro assays	762:776	In vitro assays showed that ChS/PVA-BBP does not exert cytotoxicity against healthy cells.
28024617	5	28	theme	BBP	757:759	arg1	content					746:752	the content	742:752	the content of BBP	742:759	Further, different properties of the biocomposite can be tailored according to the content of BBP.
28024617	6	29	dep	In	762:763	arg1	vitro					765:769	vitro	765:769	vitro	765:769	In vitro assays showed that ChS/PVA-BBP does not exert cytotoxicity against healthy cells.
28024617	0	30	theme	biocomposite	120:131	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
28024617	0	30	theme	biocomposite	120:131	arg1	Development					0:10	Development	0:10	Development	0:10	Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
28024617	0	30	theme	biocomposite	120:131	arg1	biocompatibility					34:49	biocompatibility	34:49	biocompatibility	34:49	Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
28024617	6	31	theme	healthy	838:844	arg1	cells					846:850	healthy cells	838:850	healthy cells	838:850	In vitro assays showed that ChS/PVA-BBP does not exert cytotoxicity against healthy cells.
28024617	7	32	theme	biocompatibility	929:944	arg1	biocomposites					911:923	ChS/PVA-BBP biocomposites	899:923	ChS/PVA-BBP biocomposites	899:923	In vivo and ex vivo experiments revealed that ChS/PVA-BBP biocomposites are biocompatibility materials without exert pro-inflammatory responses.
28024617	7	32	theme	biocompatibility	929:944	arg1	materials					946:954	biocompatibility materials	929:954	biocompatibility materials	929:954	In vivo and ex vivo experiments revealed that ChS/PVA-BBP biocomposites are biocompatibility materials without exert pro-inflammatory responses.
28024617	2	33	theme	uptake	489:494	arg1	properties					496:505	mechanical and liquid uptake properties	467:505	mechanical and liquid uptake properties	467:505	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	4	34	dep	BBP	539:541	arg1	the					543:545	the	543:545	the	543:545	% BBP the biocomposite exhibited enhanced mechanical properties and biodegradability rate as compared to the pristine sample.
28024617	4	34	dep	BBP	539:541	arg1	biocomposite					547:558	biocomposite	547:558	biocomposite	547:558	% BBP the biocomposite exhibited enhanced mechanical properties and biodegradability rate as compared to the pristine sample.
28024617	1	35	theme	vinyl	196:200	arg1	poly					191:194	poly	191:194	poly(vinyl alcohol) (PVA)	191:215	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	1	35	theme	vinyl	196:200	arg1	alcohol					202:208	vinyl alcohol	196:208	vinyl alcohol	196:208	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	2	36	theme	liquid	482:487	arg1	properties					496:505	mechanical and liquid uptake properties	467:505	mechanical and liquid uptake properties	467:505	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	5	37	theme	biocomposite	700:711	arg1	properties					682:691	different properties	672:691	different properties of the biocomposite	672:711	Further, different properties of the biocomposite can be tailored according to the content of BBP.
28024617	4	38	theme	biodegradability	605:620	arg1	rate					622:625	biodegradability rate	605:625	biodegradability rate	605:625	% BBP the biocomposite exhibited enhanced mechanical properties and biodegradability rate as compared to the pristine sample.
28024617	0	39	theme	sulfate/poly	66:77	arg1	biocomposite					120:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
28024617	9	40	theme	hydroxyapatite	1129:1142	arg1	insurance					1168:1176	insurance	1168:1176	insurance	1168:1176	Taken together, BBP is a low-cost source of hydroxyapatite and collagen, which are insurance.
28024617	9	40	theme	hydroxyapatite	1129:1142	arg1	BBP					1101:1103	BBP	1101:1103	BBP	1101:1103	Taken together, BBP is a low-cost source of hydroxyapatite and collagen, which are insurance.
28024617	9	40	theme	hydroxyapatite	1129:1142	arg1	source					1119:1124	a low-cost source	1108:1124	a low-cost source	1108:1124	Taken together, BBP is a low-cost source of hydroxyapatite and collagen, which are insurance.
28024617	0	41	theme	chondroitin	54:64	arg1	biocomposite					120:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
28024617	9	42	theme	collagen	1148:1155	arg1	insurance					1168:1176	insurance	1168:1176	insurance	1168:1176	Taken together, BBP is a low-cost source of hydroxyapatite and collagen, which are insurance.
28024617	9	42	theme	collagen	1148:1155	arg1	BBP					1101:1103	BBP	1101:1103	BBP	1101:1103	Taken together, BBP is a low-cost source of hydroxyapatite and collagen, which are insurance.
28024617	9	42	theme	collagen	1148:1155	arg1	source					1119:1124	a low-cost source	1108:1124	a low-cost source	1108:1124	Taken together, BBP is a low-cost source of hydroxyapatite and collagen, which are insurance.
28024617	1	43	theme	Chondroitin	134:144	arg1	glycosaminoglycan					172:188	a sulfated glycosaminoglycan	161:188	a sulfated glycosaminoglycan	161:188	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	1	43	theme	Chondroitin	134:144	arg1	ChS					155:157	ChS	155:157	ChS	155:157	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	1	43	theme	Chondroitin	134:144	arg1	sulfate					146:152	Chondroitin sulfate	134:152	Chondroitin sulfate (ChS)	134:158	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	5	44	theme	different	672:680	arg1	properties					682:691	different properties	672:691	different properties of the biocomposite	672:711	Further, different properties of the biocomposite can be tailored according to the content of BBP.
28024617	0	45	theme	vinyl	79:83	arg1	biocomposite					120:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite	54:131	Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
28024617	7	46	theme	ex	865:866	arg1	experiments					873:883	In vivo and ex vivo experiments	853:883	In vivo and ex vivo experiments	853:883	In vivo and ex vivo experiments revealed that ChS/PVA-BBP biocomposites are biocompatibility materials without exert pro-inflammatory responses.
28024617	4	47	theme	mechanical	579:588	arg1	properties					590:599	enhanced mechanical properties	570:599	enhanced mechanical properties	570:599	% BBP the biocomposite exhibited enhanced mechanical properties and biodegradability rate as compared to the pristine sample.
28024617	2	48	theme	remarkable	421:430	arg1	effect					432:437	a remarkable effect	419:437	a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability	419:526	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
28024617	1	49	theme	novel	269:273	arg1	biocomposite					288:299	a novel eco-friendly biocomposite	267:299	a novel eco-friendly biocomposite	267:299	Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions.
28024617	4	50	theme	enhanced	570:577	arg1	properties					590:599	enhanced mechanical properties	570:599	enhanced mechanical properties	570:599	% BBP the biocomposite exhibited enhanced mechanical properties and biodegradability rate as compared to the pristine sample.
28024617	4	51	theme	pristine	646:653	arg1	sample					655:660	the pristine sample	642:660	the pristine sample	642:660	% BBP the biocomposite exhibited enhanced mechanical properties and biodegradability rate as compared to the pristine sample.
28024617	7	52	theme	In	853:854	arg1	experiments					873:883	In vivo and ex vivo experiments	853:883	In vivo and ex vivo experiments	853:883	In vivo and ex vivo experiments revealed that ChS/PVA-BBP biocomposites are biocompatibility materials without exert pro-inflammatory responses.
28024617	2	53	theme	mechanical	467:476	arg1	properties					496:505	mechanical and liquid uptake properties	467:505	mechanical and liquid uptake properties	467:505	The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability.
27760471	1	0	dep	Lentinula	284:292	arg1	edodes					294:299	Lentinula edodes	284:299	Lentinula edodes	284:299	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	5	1	theme	microwaved	753:762	arg1	mushrooms					764:772	grilled and microwaved mushrooms	741:772	grilled and microwaved mushrooms	741:772	A significant decrease was detected in the antioxidant activity especially after boiling and frying, while grilled and microwaved mushrooms reached higher values of antioxidant activity.
27760471	1	2	theme	cultivated	243:252	arg1	mushrooms					254:262	cultivated mushrooms	243:262	cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii)	243:344	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	0	3	theme	mushrooms	96:104	arg1	activity					73:80	antioxidant activity	61:80	antioxidant activity	61:80	Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.
27760471	0	3	theme	mushrooms	96:104	arg1	value					51:55	nutritional value	39:55	nutritional value	39:55	Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.
27760471	0	4	from	Effect	0:5	arg1	activity					73:80	antioxidant activity	61:80	antioxidant activity	61:80	Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.
27760471	0	4	from	Effect	0:5	arg1	value					51:55	nutritional value	39:55	nutritional value	39:55	Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.
27760471	5	5	theme	significant	636:646	arg1	decrease					648:655	A significant decrease	634:655	A significant decrease	634:655	A significant decrease was detected in the antioxidant activity especially after boiling and frying, while grilled and microwaved mushrooms reached higher values of antioxidant activity.
27760471	1	6	dep	treatments	129:138	arg1	treatments					129:138	culinary treatments	120:138	culinary treatments (boiling, microwaving, grilling, and deep frying)	120:188	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	6	dep	treatments	129:138	arg1	grilling					163:170	grilling	163:170	grilling	163:170	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	6	dep	treatments	129:138	arg1	microwaving					150:160	microwaving	150:160	microwaving	150:160	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	6	dep	treatments	129:138	arg1	frying					182:187	deep frying	177:187	deep frying	177:187	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	6	dep	treatments	129:138	arg1	boiling					141:147	boiling	141:147	boiling	141:147	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	7	theme	mushrooms	254:262	arg1	composition					203:213	proximate composition	193:213	proximate composition	193:213	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	7	theme	mushrooms	254:262	arg1	capacity					231:238	antioxidant capacity	219:238	antioxidant capacity	219:238	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	0	8	theme	cultivated	85:94	arg1	mushrooms					96:104	cultivated mushrooms	85:104	cultivated mushrooms	85:104	Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.
27760471	1	9	dep	Pleurotus	302:310	arg1	ostreatus					312:320	Pleurotus ostreatus	302:320	Pleurotus ostreatus	302:320	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	8	10	theme	nutritional	1191:1201	arg1	profile					1203:1209	the nutritional profile	1187:1209	the nutritional profile of mushrooms	1187:1222	Microwaving and grilling were established as the best processes to maintain the nutritional profile of mushrooms.
27760471	8	11	dep	processes	1165:1173	arg1	maintain					1178:1185	maintain	1178:1185	to maintain the nutritional profile of mushrooms	1175:1222	Microwaving and grilling were established as the best processes to maintain the nutritional profile of mushrooms.
27760471	3	12	theme	carbohydrates	498:510	arg1	content					512:518	carbohydrates content	498:518	carbohydrates content	498:518	Frying induced more severe losses in protein, ash, and carbohydrates content but increased the fat and energy.
27760471	4	13	theme	total	575:579	arg1	content					589:595	the total glucans content	571:595	the total glucans content	571:595	Boiling improved the total glucans content by enhancing the β-glucans fraction.
27760471	8	14	theme	best	1160:1163	arg1	grilling					1127:1134	grilling	1127:1134	grilling	1127:1134	Microwaving and grilling were established as the best processes to maintain the nutritional profile of mushrooms.
27760471	8	14	theme	best	1160:1163	arg1	processes					1165:1173	the best processes	1156:1173	the best processes to maintain the nutritional profile of mushrooms	1156:1222	Microwaving and grilling were established as the best processes to maintain the nutritional profile of mushrooms.
27760471	8	14	theme	best	1160:1163	arg1	Microwaving					1111:1121	Microwaving	1111:1121	Microwaving	1111:1121	Microwaving and grilling were established as the best processes to maintain the nutritional profile of mushrooms.
27760471	7	15	theme	nutritional	1091:1101	arg1	losses					1103:1108	nutritional losses	1091:1108	nutritional losses	1091:1108	Since cooking techniques clearly influence the nutritional attributes of mushrooms, the proper selection of treatments is a key factor to prevent/reduce nutritional losses.
27760471	3	16	from	losses	470:475	arg1	ash					489:491	ash	489:491	ash	489:491	Frying induced more severe losses in protein, ash, and carbohydrates content but increased the fat and energy.
27760471	3	16	from	losses	470:475	arg1	protein					480:486	protein	480:486	protein	480:486	Frying induced more severe losses in protein, ash, and carbohydrates content but increased the fat and energy.
27760471	3	16	from	losses	470:475	arg1	content					512:518	carbohydrates content	498:518	carbohydrates content	498:518	Frying induced more severe losses in protein, ash, and carbohydrates content but increased the fat and energy.
27760471	2	17	theme	mushrooms	424:432	arg1	species					434:440	the mushrooms species	420:440	the mushrooms species	420:440	Proximate composition was affected by the cooking method and the mushrooms species.
27760471	1	18	dep	Pleurotus	327:335	arg1	eryngii					337:343	Pleurotus eryngii	327:343	Pleurotus eryngii	327:343	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	6	19	theme	Maillard	821:828	arg1	products					839:846	Maillard reaction products	821:846	Maillard reaction products	821:846	Maillard reaction products could be partially responsible, as supported by the absorbance values measured at 420 nm.
27760471	5	20	located	detected	661:668	arg2	decrease					648:655	A significant decrease	634:655	A significant decrease	634:655	A significant decrease was detected in the antioxidant activity especially after boiling and frying, while grilled and microwaved mushrooms reached higher values of antioxidant activity.
27760471	5	20	located	detected	661:668	arg1	activity					689:696	the antioxidant activity	673:696	the antioxidant activity	673:696	A significant decrease was detected in the antioxidant activity especially after boiling and frying, while grilled and microwaved mushrooms reached higher values of antioxidant activity.
27760471	1	21	theme	deep	177:180	arg1	treatments					129:138	culinary treatments	120:138	culinary treatments (boiling, microwaving, grilling, and deep frying)	120:188	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	21	theme	deep	177:180	arg1	frying					182:187	deep frying	177:187	deep frying	177:187	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	0	22	theme	cooking	20:26	arg1	methods					28:34	different cooking methods	10:34	different cooking methods	10:34	Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.
27760471	5	23	theme	antioxidant	799:809	arg1	activity					811:818	antioxidant activity	799:818	antioxidant activity	799:818	A significant decrease was detected in the antioxidant activity especially after boiling and frying, while grilled and microwaved mushrooms reached higher values of antioxidant activity.
27760471	5	24	theme	antioxidant	677:687	arg1	activity					689:696	the antioxidant activity	673:696	the antioxidant activity	673:696	A significant decrease was detected in the antioxidant activity especially after boiling and frying, while grilled and microwaved mushrooms reached higher values of antioxidant activity.
27760471	6	25	theme	absorbance	900:909	arg1	values					911:916	the absorbance values	896:916	the absorbance values measured at 420 nm	896:935	Maillard reaction products could be partially responsible, as supported by the absorbance values measured at 420 nm.
27760471	0	26	theme	different	10:18	arg1	methods					28:34	different cooking methods	10:34	different cooking methods	10:34	Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.
27760471	2	27	theme	cooking	401:407	arg1	method					409:414	the cooking method	397:414	the cooking method	397:414	Proximate composition was affected by the cooking method and the mushrooms species.
27760471	7	28	theme	key	1062:1064	arg1	factor					1066:1071	a key factor	1060:1071	a key factor to prevent/reduce nutritional losses	1060:1108	Since cooking techniques clearly influence the nutritional attributes of mushrooms, the proper selection of treatments is a key factor to prevent/reduce nutritional losses.
27760471	7	28	theme	key	1062:1064	arg1	selection					1033:1041	the proper selection	1022:1041	the proper selection of treatments	1022:1055	Since cooking techniques clearly influence the nutritional attributes of mushrooms, the proper selection of treatments is a key factor to prevent/reduce nutritional losses.
27760471	2	29	theme	Proximate	359:367	arg1	composition					369:379	Proximate composition	359:379	Proximate composition	359:379	Proximate composition was affected by the cooking method and the mushrooms species.
27760471	0	30	theme	methods	28:34	arg1	Effect					0:5	Effect	0:5	Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.	0:105	Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.
27760471	5	31	theme	activity	811:818	arg1	values					789:794	higher values	782:794	higher values of antioxidant activity	782:818	A significant decrease was detected in the antioxidant activity especially after boiling and frying, while grilled and microwaved mushrooms reached higher values of antioxidant activity.
27760471	7	32	theme	proper	1026:1031	arg1	factor					1066:1071	a key factor	1060:1071	a key factor to prevent/reduce nutritional losses	1060:1108	Since cooking techniques clearly influence the nutritional attributes of mushrooms, the proper selection of treatments is a key factor to prevent/reduce nutritional losses.
27760471	7	32	theme	proper	1026:1031	arg1	selection					1033:1041	the proper selection	1022:1041	the proper selection of treatments	1022:1055	Since cooking techniques clearly influence the nutritional attributes of mushrooms, the proper selection of treatments is a key factor to prevent/reduce nutritional losses.
27760471	1	33	theme	proximate	193:201	arg1	composition					203:213	proximate composition	193:213	proximate composition	193:213	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	5	34	theme	higher	782:787	arg1	values					789:794	higher values	782:794	higher values of antioxidant activity	782:818	A significant decrease was detected in the antioxidant activity especially after boiling and frying, while grilled and microwaved mushrooms reached higher values of antioxidant activity.
27760471	0	35	theme	nutritional	39:49	arg1	value					51:55	nutritional value	39:55	nutritional value	39:55	Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.
27760471	1	36	dep	mushrooms	254:262	arg1	Pleurotus					327:335	Pleurotus	327:335	Pleurotus	327:335	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	36	dep	mushrooms	254:262	arg1	Lentinula					284:292	Lentinula	284:292	Lentinula	284:292	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	36	dep	mushrooms	254:262	arg1	Pleurotus					302:310	Pleurotus	302:310	Pleurotus	302:310	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	36	dep	mushrooms	254:262	arg1	bisporus					274:281	bisporus	274:281	bisporus	274:281	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	37	theme	culinary	120:127	arg1	treatments					129:138	culinary treatments	120:138	culinary treatments (boiling, microwaving, grilling, and deep frying)	120:188	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	37	theme	culinary	120:127	arg1	grilling					163:170	grilling	163:170	grilling	163:170	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	37	theme	culinary	120:127	arg1	microwaving					150:160	microwaving	150:160	microwaving	150:160	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	37	theme	culinary	120:127	arg1	frying					182:187	deep frying	177:187	deep frying	177:187	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	37	theme	culinary	120:127	arg1	boiling					141:147	boiling	141:147	boiling	141:147	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	38	theme	treatments	129:138	arg1	Influence					107:115	Influence	107:115	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii)	107:344	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	39	from	Influence	107:115	arg1	composition					203:213	proximate composition	193:213	proximate composition	193:213	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	1	39	from	Influence	107:115	arg1	capacity					231:238	antioxidant capacity	219:238	antioxidant capacity	219:238	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	4	40	theme	glucans	581:587	arg1	content					589:595	the total glucans content	571:595	the total glucans content	571:595	Boiling improved the total glucans content by enhancing the β-glucans fraction.
27760471	7	41	theme	treatments	1046:1055	arg1	factor					1066:1071	a key factor	1060:1071	a key factor to prevent/reduce nutritional losses	1060:1108	Since cooking techniques clearly influence the nutritional attributes of mushrooms, the proper selection of treatments is a key factor to prevent/reduce nutritional losses.
27760471	7	41	theme	treatments	1046:1055	arg1	selection					1033:1041	the proper selection	1022:1041	the proper selection of treatments	1022:1055	Since cooking techniques clearly influence the nutritional attributes of mushrooms, the proper selection of treatments is a key factor to prevent/reduce nutritional losses.
27760471	6	42	theme	reaction	830:837	arg1	products					839:846	Maillard reaction products	821:846	Maillard reaction products	821:846	Maillard reaction products could be partially responsible, as supported by the absorbance values measured at 420 nm.
27760471	7	43	theme	cooking	944:950	arg1	techniques					952:961	cooking techniques	944:961	cooking techniques	944:961	Since cooking techniques clearly influence the nutritional attributes of mushrooms, the proper selection of treatments is a key factor to prevent/reduce nutritional losses.
27760471	3	44	theme	severe	463:468	arg1	losses					470:475	more severe losses	458:475	more severe losses in protein, ash, and carbohydrates content	458:518	Frying induced more severe losses in protein, ash, and carbohydrates content but increased the fat and energy.
27760471	0	45	theme	antioxidant	61:71	arg1	activity					73:80	antioxidant activity	61:80	antioxidant activity	61:80	Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.
27760471	4	46	theme	β-glucans	614:622	arg1	fraction					624:631	the β-glucans fraction	610:631	the β-glucans fraction	610:631	Boiling improved the total glucans content by enhancing the β-glucans fraction.
27760471	7	47	theme	mushrooms	1011:1019	arg1	attributes					997:1006	the nutritional attributes	981:1006	the nutritional attributes of mushrooms	981:1019	Since cooking techniques clearly influence the nutritional attributes of mushrooms, the proper selection of treatments is a key factor to prevent/reduce nutritional losses.
27760471	1	48	theme	antioxidant	219:229	arg1	capacity					231:238	antioxidant capacity	219:238	antioxidant capacity	219:238	Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied.
27760471	5	49	theme	grilled	741:747	arg1	mushrooms					764:772	grilled and microwaved mushrooms	741:772	grilled and microwaved mushrooms	741:772	A significant decrease was detected in the antioxidant activity especially after boiling and frying, while grilled and microwaved mushrooms reached higher values of antioxidant activity.
27760471	8	50	theme	mushrooms	1214:1222	arg1	profile					1203:1209	the nutritional profile	1187:1209	the nutritional profile of mushrooms	1187:1222	Microwaving and grilling were established as the best processes to maintain the nutritional profile of mushrooms.
27760471	7	51	theme	nutritional	985:995	arg1	attributes					997:1006	the nutritional attributes	981:1006	the nutritional attributes of mushrooms	981:1019	Since cooking techniques clearly influence the nutritional attributes of mushrooms, the proper selection of treatments is a key factor to prevent/reduce nutritional losses.
26253931	2	0	from	components	427:436	arg1	cartilage					452:460	knee joint cartilage	441:460	knee joint cartilage	441:460	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	9	1	from	feature	1749:1755	arg1	evaluation					1764:1773	the evaluation	1760:1773	the evaluation of the biochemical composition of knee joint cartilage	1760:1828	In the future gagCEST imaging may be an additional feature in the evaluation of the biochemical composition of knee joint cartilage.
26253931	9	1	from	feature	1749:1755	arg1	future					1705:1710	future	1705:1710	future	1705:1710	In the future gagCEST imaging may be an additional feature in the evaluation of the biochemical composition of knee joint cartilage.
26253931	2	2	theme	biochemical	359:369	arg1	transfer					337:344	glycosaminoglycan chemical exchange saturation transfer	290:344	glycosaminoglycan chemical exchange saturation transfer (gagCEST)	290:354	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	2	theme	biochemical	359:369	arg1	technique					379:387	a biochemical imaging technique	357:387	a biochemical imaging technique	357:387	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	1	3	theme	magnetic	254:261	arg1	techniques					273:282	standard magnetic resonance techniques	245:282	standard magnetic resonance techniques	245:282	BACKGROUND Biochemical alterations such as glycosaminoglycan (GAG) depletion occur early in the course of osteoarthritis, but cannot be detected with standard magnetic resonance techniques.
26253931	2	4	with	feasible	396:403	arg1	technique					379:387	a biochemical imaging technique	357:387	a biochemical imaging technique	357:387	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	4	with	feasible	396:403	arg1	gagCEST					347:353	gagCEST	347:353	gagCEST	347:353	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	4	with	feasible	396:403	arg1	transfer					337:344	glycosaminoglycan chemical exchange saturation transfer	290:344	glycosaminoglycan chemical exchange saturation transfer (gagCEST)	290:354	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	5	5	dep	patella	1148:1154	arg1	v					1145:1145	v	1145:1145	v	1145:1145	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	3	6	theme	joint	553:557	arg1	cartilage					559:567	knee joint cartilage	548:567	knee joint cartilage	548:567	PURPOSE To establish baseline values for gagCEST magnetic resonance imaging (MRI) in knee joint cartilage at 3 Tesla (T).
26253931	5	7	theme	imaging	794:800	arg1	protocol					802:809	The imaging protocol	790:809	The imaging protocol	790:809	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	8	8	dep	CONCLUSION	1568:1577	arg1	differences					1588:1598	Regional differences	1579:1598	CONCLUSION Regional differences in the gagCEST values	1568:1620	CONCLUSION Regional differences in the gagCEST values must be considered when conducting gagCEST imaging of knee joint cartilage.
26253931	7	9	theme	lateral	1517:1523	arg1	%					1553:1553	0.52% ± 0.53%	1541:1553	0.52% ± 0.53%	1541:1553	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	9	theme	lateral	1517:1523	arg1	plateau					1532:1538	the lateral tibial plateau	1513:1538	the lateral tibial plateau (0.52% ± 0.53%)	1513:1554	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	5	10	theme	cartilage	895:903	arg1	assessment					905:914	morphological cartilage assessment	881:914	morphological cartilage assessment	881:914	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	8	11	theme	gagCEST	1657:1663	arg1	imaging					1665:1671	gagCEST imaging	1657:1671	gagCEST imaging of knee joint cartilage	1657:1695	CONCLUSION Regional differences in the gagCEST values must be considered when conducting gagCEST imaging of knee joint cartilage.
26253931	2	12	theme	exchange	317:324	arg1	technique					379:387	a biochemical imaging technique	357:387	a biochemical imaging technique	357:387	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	12	theme	exchange	317:324	arg1	gagCEST					347:353	gagCEST	347:353	gagCEST	347:353	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	12	theme	exchange	317:324	arg1	transfer					337:344	glycosaminoglycan chemical exchange saturation transfer	290:344	glycosaminoglycan chemical exchange saturation transfer (gagCEST)	290:354	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	1	13	theme	osteoarthritis	201:214	arg1	course					191:196	the course	187:196	the course of osteoarthritis	187:214	BACKGROUND Biochemical alterations such as glycosaminoglycan (GAG) depletion occur early in the course of osteoarthritis, but cannot be detected with standard magnetic resonance techniques.
26253931	5	14	theme	lateral	1092:1098	arg1	plateau					1107:1113	(iii) lateral tibial plateau	1086:1113	(iii) lateral tibial plateau	1086:1113	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	5	15	theme	joint	1021:1025	arg1	regions					1001:1007	six cartilage regions	987:1007	six cartilage regions of the knee joint	987:1025	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	8	16	theme	joint	1681:1685	arg1	cartilage					1687:1695	knee joint cartilage	1676:1695	knee joint cartilage	1676:1695	CONCLUSION Regional differences in the gagCEST values must be considered when conducting gagCEST imaging of knee joint cartilage.
26253931	5	17	theme	steady-state	855:866	arg1	sequence					868:875	three-dimensional (3D) double-echo steady-state sequence	820:875	three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment	820:914	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	5	18	dep	plateau	1107:1113	arg1	iii					1087:1089	iii	1087:1089	iii	1087:1089	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	5	19	theme	tibial	1100:1105	arg1	plateau					1107:1113	(iii) lateral tibial plateau	1086:1113	(iii) lateral tibial plateau	1086:1113	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	3	20	theme	knee	548:551	arg1	cartilage					559:567	knee joint cartilage	548:567	knee joint cartilage	548:567	PURPOSE To establish baseline values for gagCEST magnetic resonance imaging (MRI) in knee joint cartilage at 3 Tesla (T).
26253931	6	21	theme	transfer	1219:1226	arg1	parameter					1244:1252	the magnetization transfer ratio (MTRasym) parameter	1201:1252	the magnetization transfer ratio (MTRasym) parameter	1201:1252	We used the asymmetry of the magnetization transfer ratio (MTRasym) parameter to quantify the gagCEST effects in these regions.
26253931	5	22	theme	femoral	1040:1046	arg1	condyle					1048:1054	(i) lateral femoral condyle	1028:1054	(i) lateral femoral condyle	1028:1054	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	4	23	dep	history	703:709	arg1	pathologies					757:767	indicative of knee joint pathologies	732:767	indicative of knee joint pathologies	732:767	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	9	24	theme	additional	1738:1747	arg1	feature					1749:1755	an additional feature	1735:1755	an additional feature in the evaluation of the biochemical composition of knee joint cartilage	1735:1828	In the future gagCEST imaging may be an additional feature in the evaluation of the biochemical composition of knee joint cartilage.
26253931	9	24	theme	additional	1738:1747	arg1	imaging					1720:1726	gagCEST imaging	1712:1726	gagCEST imaging	1712:1726	In the future gagCEST imaging may be an additional feature in the evaluation of the biochemical composition of knee joint cartilage.
26253931	5	25	theme	tibial	1128:1133	arg1	plateau					1135:1141	(iv) medial tibial plateau	1116:1141	(iv) medial tibial plateau	1116:1141	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	0	26	theme	knee	82:85	arg1	joints					87:92	asymptomatic knee joints	69:92	asymptomatic knee joints	69:92	Glycosaminoglycan chemical exchange saturation transfer at 3T MRI in asymptomatic knee joints.
26253931	4	27	theme	no	700:701	arg1	history					703:709	no history	700:709	no history	700:709	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	2	28	theme	glycosaminoglycan	290:306	arg1	technique					379:387	a biochemical imaging technique	357:387	a biochemical imaging technique	357:387	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	28	theme	glycosaminoglycan	290:306	arg1	gagCEST					347:353	gagCEST	347:353	gagCEST	347:353	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	28	theme	glycosaminoglycan	290:306	arg1	transfer					337:344	glycosaminoglycan chemical exchange saturation transfer	290:344	glycosaminoglycan chemical exchange saturation transfer (gagCEST)	290:354	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	5	29	theme	prototype	922:930	arg1	sequence					946:953	a prototype 3D CEST pulse sequence	920:953	a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint	920:1025	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	7	30	theme	medial	1470:1475	arg1	%					1506:1506	0.41% ± 0.58%	1494:1506	0.41% ± 0.58%	1494:1506	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	30	theme	medial	1470:1475	arg1	condyle					1485:1491	the medial femoral condyle	1466:1491	the medial femoral condyle (0.41% ± 0.58%)	1466:1507	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	31	theme	%	1383:1383	arg1	%					1391:1391	1.62% ± 1.19%	1379:1391	1.62% ± 1.19%	1379:1391	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	31	theme	%	1383:1383	arg1	patellar					1369:1376	patellar	1369:1376	patellar	1369:1376	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	8	32	theme	Regional	1579:1586	arg1	differences					1588:1598	Regional differences	1579:1598	CONCLUSION Regional differences in the gagCEST values	1568:1620	CONCLUSION Regional differences in the gagCEST values must be considered when conducting gagCEST imaging of knee joint cartilage.
26253931	5	33	theme	CEST	935:938	arg1	sequence					946:953	a prototype 3D CEST pulse sequence	920:953	a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint	920:1025	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	0	34	theme	asymptomatic	69:80	arg1	joints					87:92	asymptomatic knee joints	69:92	asymptomatic knee joints	69:92	Glycosaminoglycan chemical exchange saturation transfer at 3T MRI in asymptomatic knee joints.
26253931	1	35	theme	BACKGROUND	95:104	arg1	depletion					162:170	glycosaminoglycan (GAG) depletion	138:170	glycosaminoglycan (GAG) depletion	138:170	BACKGROUND Biochemical alterations such as glycosaminoglycan (GAG) depletion occur early in the course of osteoarthritis, but cannot be detected with standard magnetic resonance techniques.
26253931	1	35	theme	BACKGROUND	95:104	arg1	alterations					118:128	BACKGROUND Biochemical alterations	95:128	BACKGROUND Biochemical alterations such as glycosaminoglycan (GAG) depletion	95:170	BACKGROUND Biochemical alterations such as glycosaminoglycan (GAG) depletion occur early in the course of osteoarthritis, but cannot be detected with standard magnetic resonance techniques.
26253931	7	36	theme	MTRasym	1448:1454	arg1	values					1456:1461	low MTRasym values	1444:1461	low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages	1444:1565	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	2	37	theme	joint	446:450	arg1	cartilage					452:460	knee joint cartilage	441:460	knee joint cartilage	441:460	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	9	38	theme	composition	1794:1804	arg1	evaluation					1764:1773	the evaluation	1760:1773	the evaluation of the biochemical composition of knee joint cartilage	1760:1828	In the future gagCEST imaging may be an additional feature in the evaluation of the biochemical composition of knee joint cartilage.
26253931	5	39	dep	three-dimensional	820:836	arg1	3D					839:840	3D	839:840	3D	839:840	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	0	40	theme	exchange	27:34	arg1	transfer					47:54	exchange saturation transfer	27:54	exchange saturation transfer	27:54	Glycosaminoglycan chemical exchange saturation transfer at 3T MRI in asymptomatic knee joints.
26253931	9	41	theme	knee	1809:1812	arg1	cartilage					1820:1828	knee joint cartilage	1809:1828	knee joint cartilage	1809:1828	In the future gagCEST imaging may be an additional feature in the evaluation of the biochemical composition of knee joint cartilage.
26253931	4	42	theme	joint	751:755	arg1	indicative					732:741	indicative	732:741	indicative	732:741	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	2	43	theme	biochemical	415:425	arg1	components					427:436	biochemical components	415:436	biochemical components in knee joint cartilage	415:460	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	5	44	theme	CEST	971:974	arg1	effects					976:982	the CEST effects	967:982	the CEST effects	967:982	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	9	45	theme	cartilage	1820:1828	arg1	composition					1794:1804	the biochemical composition	1778:1804	the biochemical composition of knee joint cartilage	1778:1828	In the future gagCEST imaging may be an additional feature in the evaluation of the biochemical composition of knee joint cartilage.
26253931	3	46	theme	gagCEST	504:510	arg1	imaging					531:537	gagCEST magnetic resonance imaging	504:537	gagCEST magnetic resonance imaging (MRI) in knee joint cartilage	504:567	PURPOSE To establish baseline values for gagCEST magnetic resonance imaging (MRI) in knee joint cartilage at 3 Tesla (T).
26253931	3	46	theme	gagCEST	504:510	arg1	MRI					540:542	MRI	540:542	MRI	540:542	PURPOSE To establish baseline values for gagCEST magnetic resonance imaging (MRI) in knee joint cartilage at 3 Tesla (T).
26253931	5	47	theme	medial	1062:1067	arg1	condyle					1077:1083	(ii) medial femoral condyle	1057:1083	(ii) medial femoral condyle	1057:1083	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	7	48	theme	MTRasym	1347:1353	arg1	values					1355:1360	high MTRasym values	1342:1360	high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages	1342:1437	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	49	theme	tibial	1525:1530	arg1	%					1553:1553	0.52% ± 0.53%	1541:1553	0.52% ± 0.53%	1541:1553	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	49	theme	tibial	1525:1530	arg1	plateau					1532:1538	the lateral tibial plateau	1513:1538	the lateral tibial plateau (0.52% ± 0.53%)	1513:1554	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	6	50	from	effects	1278:1284	arg1	regions					1295:1301	these regions	1289:1301	these regions	1289:1301	We used the asymmetry of the magnetization transfer ratio (MTRasym) parameter to quantify the gagCEST effects in these regions.
26253931	8	51	from	differences	1588:1598	arg1	values					1615:1620	the gagCEST values	1603:1620	the gagCEST values	1603:1620	CONCLUSION Regional differences in the gagCEST values must be considered when conducting gagCEST imaging of knee joint cartilage.
26253931	4	52	with	volunteers	613:622	arg1	history					703:709	no history	700:709	no history	700:709	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	4	52	with	volunteers	613:622	arg1	findings					723:730	clinical findings	714:730	clinical findings	714:730	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	7	53	dep	patellar	1369:1376	arg1	cartilages					1428:1437	cartilages	1428:1437	cartilages	1428:1437	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	6	54	used	used	1179:1182	arg2	We					1176:1177	We	1176:1177	We	1176:1177	We used the asymmetry of the magnetization transfer ratio (MTRasym) parameter to quantify the gagCEST effects in these regions.
26253931	8	55	theme	gagCEST	1607:1613	arg1	values					1615:1620	the gagCEST values	1603:1620	the gagCEST values	1603:1620	CONCLUSION Regional differences in the gagCEST values must be considered when conducting gagCEST imaging of knee joint cartilage.
26253931	2	56	theme	imaging	371:377	arg1	transfer					337:344	glycosaminoglycan chemical exchange saturation transfer	290:344	glycosaminoglycan chemical exchange saturation transfer (gagCEST)	290:354	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	56	theme	imaging	371:377	arg1	technique					379:387	a biochemical imaging technique	357:387	a biochemical imaging technique	357:387	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	1	57	theme	standard	245:252	arg1	techniques					273:282	standard magnetic resonance techniques	245:282	standard magnetic resonance techniques	245:282	BACKGROUND Biochemical alterations such as glycosaminoglycan (GAG) depletion occur early in the course of osteoarthritis, but cannot be detected with standard magnetic resonance techniques.
26253931	7	58	from	values	1355:1360	arg1	%					1506:1506	0.41% ± 0.58%	1494:1506	0.41% ± 0.58%	1494:1506	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	58	from	values	1355:1360	arg1	%					1391:1391	1.62% ± 1.19%	1379:1391	1.62% ± 1.19%	1379:1391	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	58	from	values	1355:1360	arg1	patellar					1369:1376	patellar	1369:1376	patellar	1369:1376	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	58	from	values	1355:1360	arg1	condyle					1485:1491	the medial femoral condyle	1466:1491	the medial femoral condyle (0.41% ± 0.58%)	1466:1507	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	58	from	values	1355:1360	arg1	%					1425:1425	1.17% ± 1.29%	1413:1425	1.17% ± 1.29%	1413:1425	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	58	from	values	1355:1360	arg1	trochlear					1402:1410	trochlear	1402:1410	trochlear	1402:1410	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	58	from	values	1355:1360	arg1	%					1553:1553	0.52% ± 0.53%	1541:1553	0.52% ± 0.53%	1541:1553	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	58	from	values	1355:1360	arg1	plateau					1532:1538	the lateral tibial plateau	1513:1538	the lateral tibial plateau (0.52% ± 0.53%)	1513:1554	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	59	theme	%	1545:1545	arg1	%					1553:1553	0.52% ± 0.53%	1541:1553	0.52% ± 0.53%	1541:1553	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	59	theme	%	1545:1545	arg1	plateau					1532:1538	the lateral tibial plateau	1513:1538	the lateral tibial plateau (0.52% ± 0.53%)	1513:1554	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	3	60	from	imaging	531:537	arg1	cartilage					559:567	knee joint cartilage	548:567	knee joint cartilage	548:567	PURPOSE To establish baseline values for gagCEST magnetic resonance imaging (MRI) in knee joint cartilage at 3 Tesla (T).
26253931	2	61	theme	chemical	308:315	arg1	technique					379:387	a biochemical imaging technique	357:387	a biochemical imaging technique	357:387	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	61	theme	chemical	308:315	arg1	gagCEST					347:353	gagCEST	347:353	gagCEST	347:353	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	61	theme	chemical	308:315	arg1	transfer					337:344	glycosaminoglycan chemical exchange saturation transfer	290:344	glycosaminoglycan chemical exchange saturation transfer (gagCEST)	290:354	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	1	62	theme	resonance	263:271	arg1	techniques					273:282	standard magnetic resonance techniques	245:282	standard magnetic resonance techniques	245:282	BACKGROUND Biochemical alterations such as glycosaminoglycan (GAG) depletion occur early in the course of osteoarthritis, but cannot be detected with standard magnetic resonance techniques.
26253931	5	63	dep	plateau	1135:1141	arg1	iv					1117:1118	iv	1117:1118	iv	1117:1118	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	2	64	theme	saturation	326:335	arg1	technique					379:387	a biochemical imaging technique	357:387	a biochemical imaging technique	357:387	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	64	theme	saturation	326:335	arg1	gagCEST					347:353	gagCEST	347:353	gagCEST	347:353	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	2	64	theme	saturation	326:335	arg1	transfer					337:344	glycosaminoglycan chemical exchange saturation transfer	290:344	glycosaminoglycan chemical exchange saturation transfer (gagCEST)	290:354	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	3	65	theme	resonance	521:529	arg1	imaging					531:537	gagCEST magnetic resonance imaging	504:537	gagCEST magnetic resonance imaging (MRI) in knee joint cartilage	504:567	PURPOSE To establish baseline values for gagCEST magnetic resonance imaging (MRI) in knee joint cartilage at 3 Tesla (T).
26253931	3	65	theme	resonance	521:529	arg1	MRI					540:542	MRI	540:542	MRI	540:542	PURPOSE To establish baseline values for gagCEST magnetic resonance imaging (MRI) in knee joint cartilage at 3 Tesla (T).
26253931	8	66	theme	knee	1676:1679	arg1	cartilage					1687:1695	knee joint cartilage	1676:1695	knee joint cartilage	1676:1695	CONCLUSION Regional differences in the gagCEST values must be considered when conducting gagCEST imaging of knee joint cartilage.
26253931	5	67	theme	knee	1016:1019	arg1	joint					1021:1025	the knee joint	1012:1025	the knee joint	1012:1025	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	7	68	theme	%	1417:1417	arg1	%					1425:1425	1.17% ± 1.29%	1413:1425	1.17% ± 1.29%	1413:1425	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	68	theme	%	1417:1417	arg1	trochlear					1402:1410	trochlear	1402:1410	trochlear	1402:1410	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	5	69	dep	sequence	946:953	arg1	evaluate					958:965	evaluate	958:965	to evaluate the CEST effects in six cartilage regions of the knee joint	955:1025	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	8	70	theme	cartilage	1687:1695	arg1	imaging					1665:1671	gagCEST imaging	1657:1671	gagCEST imaging of knee joint cartilage	1657:1695	CONCLUSION Regional differences in the gagCEST values must be considered when conducting gagCEST imaging of knee joint cartilage.
26253931	5	71	theme	double-echo	843:853	arg1	sequence					868:875	three-dimensional (3D) double-echo steady-state sequence	820:875	three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment	820:914	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	7	72	from	values	1456:1461	arg1	%					1506:1506	0.41% ± 0.58%	1494:1506	0.41% ± 0.58%	1494:1506	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	72	from	values	1456:1461	arg1	%					1391:1391	1.62% ± 1.19%	1379:1391	1.62% ± 1.19%	1379:1391	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	72	from	values	1456:1461	arg1	patellar					1369:1376	patellar	1369:1376	patellar	1369:1376	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	72	from	values	1456:1461	arg1	condyle					1485:1491	the medial femoral condyle	1466:1491	the medial femoral condyle (0.41% ± 0.58%)	1466:1507	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	72	from	values	1456:1461	arg1	%					1425:1425	1.17% ± 1.29%	1413:1425	1.17% ± 1.29%	1413:1425	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	72	from	values	1456:1461	arg1	trochlear					1402:1410	trochlear	1402:1410	trochlear	1402:1410	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	72	from	values	1456:1461	arg1	%					1553:1553	0.52% ± 0.53%	1541:1553	0.52% ± 0.53%	1541:1553	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	72	from	values	1456:1461	arg1	plateau					1532:1538	the lateral tibial plateau	1513:1538	the lateral tibial plateau (0.52% ± 0.53%)	1513:1554	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	9	73	theme	gagCEST	1712:1718	arg1	feature					1749:1755	an additional feature	1735:1755	an additional feature in the evaluation of the biochemical composition of knee joint cartilage	1735:1828	In the future gagCEST imaging may be an additional feature in the evaluation of the biochemical composition of knee joint cartilage.
26253931	9	73	theme	gagCEST	1712:1718	arg1	imaging					1720:1726	gagCEST imaging	1712:1726	gagCEST imaging	1712:1726	In the future gagCEST imaging may be an additional feature in the evaluation of the biochemical composition of knee joint cartilage.
26253931	5	74	theme	cartilage	991:999	arg1	regions					1001:1007	six cartilage regions	987:1007	six cartilage regions of the knee joint	987:1025	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	1	75	theme	glycosaminoglycan	138:154	arg1	depletion					162:170	glycosaminoglycan (GAG) depletion	138:170	glycosaminoglycan (GAG) depletion	138:170	BACKGROUND Biochemical alterations such as glycosaminoglycan (GAG) depletion occur early in the course of osteoarthritis, but cannot be detected with standard magnetic resonance techniques.
26253931	4	76	theme	age	671:673	arg1	range					675:679	age range	671:679	age range	671:679	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	6	77	theme	ratio	1228:1232	arg1	parameter					1244:1252	the magnetization transfer ratio (MTRasym) parameter	1201:1252	the magnetization transfer ratio (MTRasym) parameter	1201:1252	We used the asymmetry of the magnetization transfer ratio (MTRasym) parameter to quantify the gagCEST effects in these regions.
26253931	4	78	dep	MATERIAL	585:592	arg1	volunteers					613:622	Twenty volunteers	606:622	Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies	606:767	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	4	78	dep	MATERIAL	585:592	arg1	women					627:631	8 women	625:631	8 women	625:631	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	5	79	theme	three-dimensional	820:836	arg1	sequence					868:875	three-dimensional (3D) double-echo steady-state sequence	820:875	three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment	820:914	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	4	80	theme	mean	642:645	arg1	age					647:649	mean age	642:649	mean age	642:649	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	5	81	theme	medial	1121:1126	arg1	plateau					1135:1141	(iv) medial tibial plateau	1116:1141	(iv) medial tibial plateau	1116:1141	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	6	82	theme	magnetization	1205:1217	arg1	parameter					1244:1252	the magnetization transfer ratio (MTRasym) parameter	1201:1252	the magnetization transfer ratio (MTRasym) parameter	1201:1252	We used the asymmetry of the magnetization transfer ratio (MTRasym) parameter to quantify the gagCEST effects in these regions.
26253931	7	83	theme	%	1498:1498	arg1	%					1506:1506	0.41% ± 0.58%	1494:1506	0.41% ± 0.58%	1494:1506	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	83	theme	%	1498:1498	arg1	condyle					1485:1491	the medial femoral condyle	1466:1491	the medial femoral condyle (0.41% ± 0.58%)	1466:1507	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	84	theme	1.62	1379:1382	arg1	%					1391:1391	1.62% ± 1.19%	1379:1391	1.62% ± 1.19%	1379:1391	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	84	theme	1.62	1379:1382	arg1	patellar					1369:1376	patellar	1369:1376	patellar	1369:1376	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	0	85	from	MRI	62:64	arg1	joints					87:92	asymptomatic knee joints	69:92	asymptomatic knee joints	69:92	Glycosaminoglycan chemical exchange saturation transfer at 3T MRI in asymptomatic knee joints.
26253931	5	86	theme	3D	932:933	arg1	sequence					946:953	a prototype 3D CEST pulse sequence	920:953	a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint	920:1025	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	7	87	theme	femoral	1477:1483	arg1	%					1506:1506	0.41% ± 0.58%	1494:1506	0.41% ± 0.58%	1494:1506	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	87	theme	femoral	1477:1483	arg1	condyle					1485:1491	the medial femoral condyle	1466:1491	the medial femoral condyle (0.41% ± 0.58%)	1466:1507	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	7	88	dep	condyle	1485:1491	arg1	cartilages					1556:1565	cartilages	1556:1565	cartilages	1556:1565	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	6	89	theme	gagCEST	1270:1276	arg1	effects					1278:1284	the gagCEST effects	1266:1284	the gagCEST effects in these regions	1266:1301	We used the asymmetry of the magnetization transfer ratio (MTRasym) parameter to quantify the gagCEST effects in these regions.
26253931	5	90	theme	lateral	1032:1038	arg1	condyle					1048:1054	(i) lateral femoral condyle	1028:1054	(i) lateral femoral condyle	1028:1054	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	5	91	theme	morphological	881:893	arg1	assessment					905:914	morphological cartilage assessment	881:914	morphological cartilage assessment	881:914	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	9	92	theme	biochemical	1782:1792	arg1	composition					1794:1804	the biochemical composition	1778:1804	the biochemical composition of knee joint cartilage	1778:1828	In the future gagCEST imaging may be an additional feature in the evaluation of the biochemical composition of knee joint cartilage.
26253931	0	93	theme	saturation	36:45	arg1	transfer					47:54	exchange saturation transfer	27:54	exchange saturation transfer	27:54	Glycosaminoglycan chemical exchange saturation transfer at 3T MRI in asymptomatic knee joints.
26253931	7	94	dep	RESULTS	1304:1310	arg1	revealed					1333:1340	revealed	1333:1340	revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages	1333:1565	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	1	95	theme	Biochemical	106:116	arg1	depletion					162:170	glycosaminoglycan (GAG) depletion	138:170	glycosaminoglycan (GAG) depletion	138:170	BACKGROUND Biochemical alterations such as glycosaminoglycan (GAG) depletion occur early in the course of osteoarthritis, but cannot be detected with standard magnetic resonance techniques.
26253931	1	95	theme	Biochemical	106:116	arg1	alterations					118:128	BACKGROUND Biochemical alterations	95:128	BACKGROUND Biochemical alterations such as glycosaminoglycan (GAG) depletion	95:170	BACKGROUND Biochemical alterations such as glycosaminoglycan (GAG) depletion occur early in the course of osteoarthritis, but cannot be detected with standard magnetic resonance techniques.
26253931	2	96	theme	knee	441:444	arg1	cartilage					452:460	knee joint cartilage	441:460	knee joint cartilage	441:460	With glycosaminoglycan chemical exchange saturation transfer (gagCEST), a biochemical imaging technique, it is feasible to detect biochemical components in knee joint cartilage.
26253931	3	97	theme	baseline	484:491	arg1	values					493:498	baseline values	484:498	baseline values for gagCEST magnetic resonance imaging (MRI) in knee joint cartilage	484:567	PURPOSE To establish baseline values for gagCEST magnetic resonance imaging (MRI) in knee joint cartilage at 3 Tesla (T).
26253931	6	98	theme	parameter	1244:1252	arg1	asymmetry					1188:1196	the asymmetry	1184:1196	the asymmetry of the magnetization transfer ratio (MTRasym) parameter	1184:1252	We used the asymmetry of the magnetization transfer ratio (MTRasym) parameter to quantify the gagCEST effects in these regions.
26253931	4	99	dep	women	627:631	arg1	years					688:692	21-29 years	682:692	21-29 years	682:692	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	4	99	dep	women	627:631	arg1	men					637:639	12 men	634:639	12 men	634:639	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	4	99	dep	women	627:631	arg1	age					647:649	mean age	642:649	mean age	642:649	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	4	99	dep	women	627:631	arg1	years					665:669	24.55 ± 2.35 years	652:669	24.55 ± 2.35 years	652:669	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	4	99	dep	women	627:631	arg1	range					675:679	age range	671:679	age range	671:679	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	7	100	theme	low	1444:1446	arg1	values					1456:1461	low MTRasym values	1444:1461	low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages	1444:1565	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	9	101	theme	joint	1814:1818	arg1	cartilage					1820:1828	knee joint cartilage	1809:1828	knee joint cartilage	1809:1828	In the future gagCEST imaging may be an additional feature in the evaluation of the biochemical composition of knee joint cartilage.
26253931	4	102	theme	knee	746:749	arg1	joint					751:755	knee joint	746:755	knee joint	746:755	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	6	103	theme	MTRasym	1235:1241	arg1	parameter					1244:1252	the magnetization transfer ratio (MTRasym) parameter	1201:1252	the magnetization transfer ratio (MTRasym) parameter	1201:1252	We used the asymmetry of the magnetization transfer ratio (MTRasym) parameter to quantify the gagCEST effects in these regions.
26253931	7	104	theme	Regional	1312:1319	arg1	differences					1321:1331	Regional differences	1312:1331	Regional differences	1312:1331	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	3	105	theme	magnetic	512:519	arg1	imaging					531:537	gagCEST magnetic resonance imaging	504:537	gagCEST magnetic resonance imaging (MRI) in knee joint cartilage	504:567	PURPOSE To establish baseline values for gagCEST magnetic resonance imaging (MRI) in knee joint cartilage at 3 Tesla (T).
26253931	3	105	theme	magnetic	512:519	arg1	MRI					540:542	MRI	540:542	MRI	540:542	PURPOSE To establish baseline values for gagCEST magnetic resonance imaging (MRI) in knee joint cartilage at 3 Tesla (T).
26253931	5	106	theme	femoral	1069:1075	arg1	condyle					1077:1083	(ii) medial femoral condyle	1057:1083	(ii) medial femoral condyle	1057:1083	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	5	107	theme	pulse	940:944	arg1	sequence					946:953	a prototype 3D CEST pulse sequence	920:953	a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint	920:1025	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	5	108	dep	trochlea	1166:1173	arg1	vi					1162:1163	vi	1162:1163	vi	1162:1163	The imaging protocol included three-dimensional (3D) double-echo steady-state sequence for morphological cartilage assessment and a prototype 3D CEST pulse sequence to evaluate the CEST effects in six cartilage regions of the knee joint: (i) lateral femoral condyle; (ii) medial femoral condyle; (iii) lateral tibial plateau; (iv) medial tibial plateau; (v) patella; and (vi) trochlea.
26253931	4	109	theme	clinical	714:721	arg1	findings					723:730	clinical findings	714:730	clinical findings	714:730	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
26253931	7	110	theme	high	1342:1345	arg1	values					1355:1360	high MTRasym values	1342:1360	high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages	1342:1437	RESULTS Regional differences revealed high MTRasym values in the patellar (1.62% ± 1.19%) and the trochlear (1.17% ± 1.29%) cartilages, and low MTRasym values in the medial femoral condyle (0.41% ± 0.58%) and the lateral tibial plateau (0.52% ± 0.53%) cartilages.
26253931	4	111	theme	indicative	732:741	arg1	pathologies					757:767	indicative of knee joint pathologies	732:767	indicative of knee joint pathologies	732:767	MATERIAL AND METHODS Twenty volunteers (8 women, 12 men; mean age, 24.55 ± 2.35 years;age range, 21-29 years) with no history or clinical findings indicative of knee joint pathologies underwent MRI at 3T.
24748509	11	0	theme	former	1643:1648	arg1	factors					1654:1660	the former two factors	1639:1660	the former two factors	1639:1660	The effect of carbohydrate type was small compared with the former two factors.
24748509	12	1	theme	in	1690:1691	arg1	metabolism					1699:1708	in vitro metabolism	1690:1708	in vitro metabolism of 22:6n-3	1690:1719	These results suggest that in vitro metabolism of 22:6n-3 is mostly dictated by the conditions at the start of the incubation, i.e., inoculum, probably reflecting the density of bacteria able to metabolize 22:6n-3.
24748509	5	2	theme	carbohydrate	751:762	arg1	type					764:767	carbohydrate type	751:767	carbohydrate type (cellulose, glucose, cellobiose and starch)	751:811	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	9	3	theme	22:6n-3	1463:1469	arg1	22:6n-3					1463:1469	22:6n-3	1463:1469	22:6n-3	1463:1469	Low levels of 22:6n-3 (0.05 mg/mL) allowed extensive metabolism whereas increasing amounts of 22:6n-3 hampered its disappearance.
24748509	9	3	theme	22:6n-3	1463:1469	arg1	amounts					1452:1458	increasing amounts	1441:1458	increasing amounts of 22:6n-3	1441:1469	Low levels of 22:6n-3 (0.05 mg/mL) allowed extensive metabolism whereas increasing amounts of 22:6n-3 hampered its disappearance.
24748509	8	4	theme	rumen	1271:1275	arg1	fluid					1277:1281	0.1 to 0.4 mg/mL rumen fluid	1254:1281	0.1 to 0.4 mg/mL rumen fluid	1254:1281	In this experiment, 22:6n-3 was added as a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid, contrary to former experiments where concentrations were relative to culture medium.
24748509	9	5	theme	increasing	1441:1450	arg1	22:6n-3					1463:1469	22:6n-3	1463:1469	22:6n-3	1463:1469	Low levels of 22:6n-3 (0.05 mg/mL) allowed extensive metabolism whereas increasing amounts of 22:6n-3 hampered its disappearance.
24748509	9	5	theme	increasing	1441:1450	arg1	amounts					1452:1458	increasing amounts	1441:1458	increasing amounts of 22:6n-3	1441:1469	Low levels of 22:6n-3 (0.05 mg/mL) allowed extensive metabolism whereas increasing amounts of 22:6n-3 hampered its disappearance.
24748509	1	6	theme	Ruminal	148:154	arg1	disappearance					156:168	Ruminal disappearance	148:168	Ruminal disappearance of linoleic and linolenic acid	148:199	Ruminal disappearance of linoleic and linolenic acid has been studied extensively.
24748509	8	7	dep	mg/mL	1265:1269	arg1	0.4					1261:1263	0.4	1261:1263	0.4	1261:1263	In this experiment, 22:6n-3 was added as a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid, contrary to former experiments where concentrations were relative to culture medium.
24748509	9	8	theme	22:6n-3	1383:1389	arg1	levels					1373:1378	Low levels	1369:1378	Low levels of 22:6n-3 (0.05 mg/mL)	1369:1402	Low levels of 22:6n-3 (0.05 mg/mL) allowed extensive metabolism whereas increasing amounts of 22:6n-3 hampered its disappearance.
24748509	10	9	theme	22:6n-3	1574:1580	arg1	disappearance					1557:1569	increased disappearance	1547:1569	increased disappearance of 22:6n-3	1547:1580	A greater proportion of rumen fluid resulted in increased disappearance of 22:6n-3.
24748509	2	10	theme	acid	282:285	arg1	metabolism					252:261	the metabolism	248:261	the metabolism of docosahexaenoic acid (22:6n-3)	248:295	Less is known of the metabolism of docosahexaenoic acid (22:6n-3).
24748509	4	11	theme	substrate	524:532	arg1	effect					471:476	the effect	467:476	the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h)	467:666	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	0	12	theme	inoculum	75:82	arg1	size					84:87	inoculum size	75:87	inoculum size	75:87	Rumen metabolism of 22:6n-3 in vitro is dependent on its concentration and inoculum size, but less dependent on substrate carbohydrate composition.
24748509	6	13	theme	cellulose	1010:1018	arg1	combinations					971:982	combinations	971:982	combinations of cellobiose, starch and cellulose	971:1018	In experiment 3, several concentrations of 22:6n-3 (0.05-0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose).
24748509	5	14	from	effect	741:746	arg1	mg/mL					844:848	0.08 mg/mL	839:848	0.08 mg/mL	839:848	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	5	14	from	effect	741:746	arg1	disappearance					824:836	22:6n-3 disappearance	816:836	22:6n-3 disappearance (0.08 mg/mL)	816:849	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	2	15	theme	docosahexaenoic	266:280	arg1	22:6n-3					288:294	22:6n-3	288:294	22:6n-3	288:294	Less is known of the metabolism of docosahexaenoic acid (22:6n-3).
24748509	2	15	theme	docosahexaenoic	266:280	arg1	acid					282:285	docosahexaenoic acid	266:285	docosahexaenoic acid (22:6n-3)	266:295	Less is known of the metabolism of docosahexaenoic acid (22:6n-3).
24748509	3	16	theme	study	314:318	arg1	aim					302:304	The aim	298:304	The aim of this study	298:318	The aim of this study was to identify factors which affect the disappearance of 22:6n-3 during in vitro batch incubations using rumen fluid from sheep.
24748509	12	17	from	start	1765:1769	arg1	conditions					1747:1756	the conditions	1743:1756	the conditions	1743:1756	These results suggest that in vitro metabolism of 22:6n-3 is mostly dictated by the conditions at the start of the incubation, i.e., inoculum, probably reflecting the density of bacteria able to metabolize 22:6n-3.
24748509	12	18	theme	bacteria	1841:1848	arg1	density					1830:1836	the density	1826:1836	the density of bacteria able to metabolize 22:6n-3	1826:1875	These results suggest that in vitro metabolism of 22:6n-3 is mostly dictated by the conditions at the start of the incubation, i.e., inoculum, probably reflecting the density of bacteria able to metabolize 22:6n-3.
24748509	5	19	used	used	704:707	arg2	design					693:698	A mixture design	683:698	A mixture design	683:698	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	1	20	theme	linoleic	173:180	arg1	disappearance					156:168	Ruminal disappearance	148:168	Ruminal disappearance of linoleic and linolenic acid	148:199	Ruminal disappearance of linoleic and linolenic acid has been studied extensively.
24748509	3	21	theme	batch	402:406	arg1	incubations					408:418	in vitro batch incubations	393:418	in vitro batch incubations using rumen fluid from sheep	393:447	The aim of this study was to identify factors which affect the disappearance of 22:6n-3 during in vitro batch incubations using rumen fluid from sheep.
24748509	5	22	theme	type	764:767	arg1	effect					741:746	the effect	737:746	the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL)	737:849	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	12	23	theme	22:6n-3	1713:1719	arg1	metabolism					1699:1708	in vitro metabolism	1690:1708	in vitro metabolism of 22:6n-3	1690:1719	These results suggest that in vitro metabolism of 22:6n-3 is mostly dictated by the conditions at the start of the incubation, i.e., inoculum, probably reflecting the density of bacteria able to metabolize 22:6n-3.
24748509	3	24	from	sheep	443:447	arg1	fluid					432:436	rumen fluid	426:436	rumen fluid from sheep	426:447	The aim of this study was to identify factors which affect the disappearance of 22:6n-3 during in vitro batch incubations using rumen fluid from sheep.
24748509	6	25	theme	starch	999:1004	arg1	combinations					971:982	combinations	971:982	combinations of cellobiose, starch and cellulose	971:1018	In experiment 3, several concentrations of 22:6n-3 (0.05-0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose).
24748509	4	26	theme	22:6n-3	576:582	arg1	addition					584:591	22:6n-3 addition	576:591	22:6n-3 addition	576:591	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	10	27	theme	fluid	1529:1533	arg1	proportion					1509:1518	A greater proportion	1499:1518	A greater proportion of rumen fluid	1499:1533	A greater proportion of rumen fluid resulted in increased disappearance of 22:6n-3.
24748509	4	28	dep	ratio	504:508	arg1	0.4					518:520	0.4	518:520	0.4	518:520	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	4	28	dep	ratio	504:508	arg1	0.2					511:513	0.2	511:513	0.2	511:513	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	8	29	theme	culture	1353:1359	arg1	medium					1361:1366	culture medium	1353:1366	culture medium	1353:1366	In this experiment, 22:6n-3 was added as a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid, contrary to former experiments where concentrations were relative to culture medium.
24748509	10	30	theme	increased	1547:1555	arg1	disappearance					1557:1569	increased disappearance	1547:1569	increased disappearance of 22:6n-3	1547:1580	A greater proportion of rumen fluid resulted in increased disappearance of 22:6n-3.
24748509	3	31	theme	in	393:394	arg1	incubations					408:418	in vitro batch incubations	393:418	in vitro batch incubations using rumen fluid from sheep	393:447	The aim of this study was to identify factors which affect the disappearance of 22:6n-3 during in vitro batch incubations using rumen fluid from sheep.
24748509	0	32	theme	Rumen	0:4	arg1	metabolism					6:15	Rumen metabolism	0:15	Rumen metabolism of 22:6n-3 in vitro	0:35	Rumen metabolism of 22:6n-3 in vitro is dependent on its concentration and inoculum size, but less dependent on substrate carbohydrate composition.
24748509	12	33	dep	inoculum	1796:1803	arg1	i.e.					1790:1793	i.e.	1790:1793	i.e.	1790:1793	These results suggest that in vitro metabolism of 22:6n-3 is mostly dictated by the conditions at the start of the incubation, i.e., inoculum, probably reflecting the density of bacteria able to metabolize 22:6n-3.
24748509	5	34	dep	type	764:767	arg1	cellulose					770:778	cellulose	770:778	cellulose	770:778	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	5	34	dep	type	764:767	arg1	starch					805:810	starch	805:810	starch	805:810	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	5	34	dep	type	764:767	arg1	cellobiose					790:799	cellobiose	790:799	cellobiose	790:799	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	5	34	dep	type	764:767	arg1	glucose					781:787	glucose	781:787	glucose	781:787	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	6	35	theme	22:6n-3	895:901	arg1	concentrations					877:890	several concentrations	869:890	several concentrations of 22:6n-3 (0.05-0.30 mg/mL)	869:919	In experiment 3, several concentrations of 22:6n-3 (0.05-0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose).
24748509	6	35	theme	22:6n-3	895:901	arg1	mg/mL					914:918	0.05-0.30 mg/mL	904:918	0.05-0.30 mg/mL	904:918	In experiment 3, several concentrations of 22:6n-3 (0.05-0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose).
24748509	0	36	theme	22:6n-3	20:26	arg1	metabolism					6:15	Rumen metabolism	0:15	Rumen metabolism of 22:6n-3 in vitro	0:35	Rumen metabolism of 22:6n-3 in vitro is dependent on its concentration and inoculum size, but less dependent on substrate carbohydrate composition.
24748509	4	37	theme	ratio	504:508	arg1	effect					471:476	the effect	467:476	the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h)	467:666	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	4	38	theme	time	650:653	arg1	effect					471:476	the effect	467:476	the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h)	467:666	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	1	39	theme	linolenic	186:194	arg1	acid					196:199	linolenic acid	186:199	linolenic acid	186:199	Ruminal disappearance of linoleic and linolenic acid has been studied extensively.
24748509	6	40	theme	cellobiose	987:996	arg1	combinations					971:982	combinations	971:982	combinations of cellobiose, starch and cellulose	971:1018	In experiment 3, several concentrations of 22:6n-3 (0.05-0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose).
24748509	5	41	theme	22:6n-3	816:822	arg1	mg/mL					844:848	0.08 mg/mL	839:848	0.08 mg/mL	839:848	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	5	41	theme	22:6n-3	816:822	arg1	disappearance					824:836	22:6n-3 disappearance	816:836	22:6n-3 disappearance (0.08 mg/mL)	816:849	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	4	42	dep	substrate	524:532	arg1	cellulose					535:543	cellulose	535:543	cellulose	535:543	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	4	42	dep	substrate	524:532	arg1	cellulose/glucose					548:564	cellulose/glucose	548:564	cellulose/glucose	548:564	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	4	43	theme	incubation	639:648	arg1	h					665:665	24 or 48 h	656:665	24 or 48 h	656:665	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	4	43	theme	incubation	639:648	arg1	time					650:653	incubation time	639:653	incubation time (24 or 48 h)	639:666	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	10	44	theme	rumen	1523:1527	arg1	fluid					1529:1533	rumen fluid	1523:1533	rumen fluid	1523:1533	A greater proportion of rumen fluid resulted in increased disappearance of 22:6n-3.
24748509	1	45	theme	acid	196:199	arg1	disappearance					156:168	Ruminal disappearance	148:168	Ruminal disappearance of linoleic and linolenic acid	148:199	Ruminal disappearance of linoleic and linolenic acid has been studied extensively.
24748509	3	46	theme	22:6n-3	378:384	arg1	disappearance					361:373	the disappearance	357:373	the disappearance of 22:6n-3	357:384	The aim of this study was to identify factors which affect the disappearance of 22:6n-3 during in vitro batch incubations using rumen fluid from sheep.
24748509	8	47	dep	fluid	1277:1281	arg1	mg/mL					1265:1269	mg/mL	1265:1269	mg/mL	1265:1269	In this experiment, 22:6n-3 was added as a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid, contrary to former experiments where concentrations were relative to culture medium.
24748509	0	48	theme	substrate	112:120	arg1	composition					135:145	substrate carbohydrate composition	112:145	substrate carbohydrate composition	112:145	Rumen metabolism of 22:6n-3 in vitro is dependent on its concentration and inoculum size, but less dependent on substrate carbohydrate composition.
24748509	3	49	dep	in	393:394	arg1	vitro					396:400	vitro	396:400	vitro	396:400	The aim of this study was to identify factors which affect the disappearance of 22:6n-3 during in vitro batch incubations using rumen fluid from sheep.
24748509	7	50	theme	substrate	1114:1122	arg1	effect					1049:1054	the effect	1045:1054	the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch)	1045:1155	In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated.
24748509	6	51	theme	several	869:875	arg1	concentrations					877:890	several concentrations	869:890	several concentrations of 22:6n-3 (0.05-0.30 mg/mL)	869:919	In experiment 3, several concentrations of 22:6n-3 (0.05-0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose).
24748509	6	51	theme	several	869:875	arg1	mg/mL					914:918	0.05-0.30 mg/mL	904:918	0.05-0.30 mg/mL	904:918	In experiment 3, several concentrations of 22:6n-3 (0.05-0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose).
24748509	4	52	theme	addition	584:591	arg1	time					568:571	time	568:571	time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation)	568:633	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	4	52	theme	addition	584:591	arg1	substrate					524:532	substrate	524:532	substrate (cellulose or cellulose/glucose)	524:565	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	4	52	theme	addition	584:591	arg1	h					665:665	24 or 48 h	656:665	24 or 48 h	656:665	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	4	52	theme	addition	584:591	arg1	time					650:653	incubation time	639:653	incubation time (24 or 48 h)	639:666	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	4	52	theme	addition	584:591	arg1	ratio					504:508	the rumen fluid/buffer ratio	481:508	the rumen fluid/buffer ratio (0.2 or 0.4)	481:521	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	11	53	theme	carbohydrate	1597:1608	arg1	type					1610:1613	carbohydrate type	1597:1613	carbohydrate type	1597:1613	The effect of carbohydrate type was small compared with the former two factors.
24748509	8	54	theme	rumen	1229:1233	arg1	fluid					1235:1239	rumen fluid	1229:1239	rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid	1229:1281	In this experiment, 22:6n-3 was added as a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid, contrary to former experiments where concentrations were relative to culture medium.
24748509	4	55	dep	time	568:571	arg1	mg/mL					599:603	0.08 mg/mL	594:603	0.08 mg/mL after 0 or 6 h of incubation	594:632	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	3	56	theme	rumen	426:430	arg1	fluid					432:436	rumen fluid	426:436	rumen fluid from sheep	426:447	The aim of this study was to identify factors which affect the disappearance of 22:6n-3 during in vitro batch incubations using rumen fluid from sheep.
24748509	12	57	dep	in	1690:1691	arg1	vitro					1693:1697	vitro	1693:1697	vitro	1693:1697	These results suggest that in vitro metabolism of 22:6n-3 is mostly dictated by the conditions at the start of the incubation, i.e., inoculum, probably reflecting the density of bacteria able to metabolize 22:6n-3.
24748509	11	58	theme	type	1610:1613	arg1	small					1619:1623	small	1619:1623	small	1619:1623	The effect of carbohydrate type was small compared with the former two factors.
24748509	11	58	theme	type	1610:1613	arg1	effect					1587:1592	The effect	1583:1592	The effect of carbohydrate type	1583:1613	The effect of carbohydrate type was small compared with the former two factors.
24748509	8	59	theme	former	1296:1301	arg1	experiments					1303:1313	former experiments	1296:1313	former experiments where concentrations were relative to culture medium	1296:1366	In this experiment, 22:6n-3 was added as a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid, contrary to former experiments where concentrations were relative to culture medium.
24748509	9	60	theme	Low	1369:1371	arg1	levels					1373:1378	Low levels	1369:1378	Low levels of 22:6n-3 (0.05 mg/mL)	1369:1402	Low levels of 22:6n-3 (0.05 mg/mL) allowed extensive metabolism whereas increasing amounts of 22:6n-3 hampered its disappearance.
24748509	4	61	theme	fluid/buffer	491:502	arg1	ratio					504:508	the rumen fluid/buffer ratio	481:508	the rumen fluid/buffer ratio (0.2 or 0.4)	481:521	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	0	62	theme	carbohydrate	122:133	arg1	composition					135:145	substrate carbohydrate composition	112:145	substrate carbohydrate composition	112:145	Rumen metabolism of 22:6n-3 in vitro is dependent on its concentration and inoculum size, but less dependent on substrate carbohydrate composition.
24748509	5	63	theme	mixture	685:691	arg1	design					693:698	A mixture design	683:698	A mixture design	683:698	A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL).
24748509	9	64	theme	extensive	1412:1420	arg1	metabolism					1422:1431	extensive metabolism	1412:1431	extensive metabolism	1412:1431	Low levels of 22:6n-3 (0.05 mg/mL) allowed extensive metabolism whereas increasing amounts of 22:6n-3 hampered its disappearance.
24748509	4	65	theme	rumen	485:489	arg1	ratio					504:508	the rumen fluid/buffer ratio	481:508	the rumen fluid/buffer ratio (0.2 or 0.4)	481:521	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	8	66	theme	fluid	1235:1239	arg1	proportion					1215:1224	a proportion	1213:1224	a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid	1213:1281	In this experiment, 22:6n-3 was added as a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid, contrary to former experiments where concentrations were relative to culture medium.
24748509	8	66	theme	fluid	1235:1239	arg1	22:6n-3					1192:1198	22:6n-3	1192:1198	22:6n-3	1192:1198	In this experiment, 22:6n-3 was added as a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid, contrary to former experiments where concentrations were relative to culture medium.
24748509	7	67	dep	substrate	1114:1122	arg1	starch					1149:1154	starch	1149:1154	starch	1149:1154	In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated.
24748509	7	67	dep	substrate	1114:1122	arg1	cellobiose					1134:1143	cellobiose	1134:1143	cellobiose	1134:1143	In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated.
24748509	7	67	dep	substrate	1114:1122	arg1	glucose					1125:1131	glucose	1125:1131	glucose	1125:1131	In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated.
24748509	4	68	theme	incubation	623:632	arg1	h					618:618	0 or 6 h	611:618	0 or 6 h of incubation	611:632	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	10	69	theme	greater	1501:1507	arg1	proportion					1509:1518	A greater proportion	1499:1518	A greater proportion of rumen fluid	1499:1533	A greater proportion of rumen fluid resulted in increased disappearance of 22:6n-3.
24748509	7	70	dep	ratio	1082:1086	arg1	0.50					1104:1107	0.50	1104:1107	0.50	1104:1107	In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated.
24748509	7	70	dep	ratio	1082:1086	arg1	0.35					1095:1098	0.35	1095:1098	0.35	1095:1098	In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated.
24748509	6	71	theme	different	941:949	arg1	mixtures					961:968	different substrate mixtures	941:968	different substrate mixtures (combinations of cellobiose, starch and cellulose)	941:1019	In experiment 3, several concentrations of 22:6n-3 (0.05-0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose).
24748509	6	72	theme	substrate	951:959	arg1	mixtures					961:968	different substrate mixtures	941:968	different substrate mixtures (combinations of cellobiose, starch and cellulose)	941:1019	In experiment 3, several concentrations of 22:6n-3 (0.05-0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose).
24748509	12	73	theme	incubation	1778:1787	arg1	start					1765:1769	the start	1761:1769	the start of the incubation	1761:1787	These results suggest that in vitro metabolism of 22:6n-3 is mostly dictated by the conditions at the start of the incubation, i.e., inoculum, probably reflecting the density of bacteria able to metabolize 22:6n-3.
24748509	4	74	theme	time	568:571	arg1	effect					471:476	the effect	467:476	the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h)	467:666	In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated.
24748509	12	75	theme	able	1850:1853	arg1	bacteria					1841:1848	bacteria	1841:1848	bacteria able to metabolize 22:6n-3	1841:1875	These results suggest that in vitro metabolism of 22:6n-3 is mostly dictated by the conditions at the start of the incubation, i.e., inoculum, probably reflecting the density of bacteria able to metabolize 22:6n-3.
24748509	7	76	theme	rumen	1063:1067	arg1	ratio					1082:1086	the rumen fluid/buffer ratio	1059:1086	the rumen fluid/buffer ratio (0.20, 0.35 and 0.50)	1059:1108	In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated.
24748509	8	77	dep	0.4	1261:1263	arg1	to					1258:1259	to	1258:1259	to	1258:1259	In this experiment, 22:6n-3 was added as a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid, contrary to former experiments where concentrations were relative to culture medium.
24748509	6	78	dep	mixtures	961:968	arg1	combinations					971:982	combinations	971:982	combinations of cellobiose, starch and cellulose	971:1018	In experiment 3, several concentrations of 22:6n-3 (0.05-0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose).
24748509	7	79	theme	ratio	1082:1086	arg1	effect					1049:1054	the effect	1045:1054	the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch)	1045:1155	In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated.
24748509	7	80	theme	fluid/buffer	1069:1080	arg1	ratio					1082:1086	the rumen fluid/buffer ratio	1059:1086	the rumen fluid/buffer ratio (0.20, 0.35 and 0.50)	1059:1108	In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated.
24748509	7	81	theme	final	1027:1031	arg1	experiment					1033:1042	a final experiment	1025:1042	a final experiment	1025:1042	In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated.
26718869	8	0	theme	oral	1133:1136	arg1	material					1156:1163	a new generation oral denture composite material	1116:1163	a new generation oral denture composite material	1116:1163	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	7	1	contain	had	913:915	arg2	degrees					927:933	different degrees	917:933	different degrees of corrosion resistance and antimicrobial properties	917:986	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	7	1	contain	had	913:915	arg1	structures					839:848	The two structures	831:848	The two structures of chitosan quaternary ammonium salt-modified denture material	831:911	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	1	2	from	solubility	205:214	arg1	water					219:223	water	219:223	water	219:223	Chitosan quaternary ammonium salt displays good antioxidant and antibacterial characteristics and it shows appreciable solubility in water.
26718869	4	3	theme	artificial	515:524	arg1	saliva					526:531	artificial saliva	515:531	artificial saliva	515:531	After three months of immersion in artificial saliva, the specimens were tested for tensile strength and were scanned by electron microscope.
26718869	5	4	theme	antibacterial	661:673	arg1	properties					675:684	antibacterial properties	661:684	antibacterial properties	661:684	The murine fibroblast cytotoxicity and antibacterial properties were also tested.
26718869	8	5	theme	ammonium	1037:1044	arg1	material					1079:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	7	6	theme	material	904:911	arg1	structures					839:848	The two structures	831:848	The two structures of chitosan quaternary ammonium salt-modified denture material	831:911	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	8	7	theme	denture	1066:1072	arg1	material					1079:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	8	8	theme	resin	1060:1064	arg1	material					1079:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	3	9	theme	ammonium	399:406	arg1	salt					408:411	chitosan quaternary ammonium salt	379:411	chitosan quaternary ammonium salt	379:411	In this study, chitosan quaternary ammonium salt was added to the denture material following two different methods.
26718869	6	10	theme	cells	824:828	arg1	proliferation					784:796	the proliferation	780:796	the proliferation of murine L929 fibroblast cells	780:828	The result showed no significant differences in the tensile strength and in the proliferation of murine L929 fibroblast cells.
26718869	6	11	theme	significant	725:735	arg1	differences					737:747	no significant differences	722:747	no significant differences	722:747	The result showed no significant differences in the tensile strength and in the proliferation of murine L929 fibroblast cells.
26718869	2	12	theme	oral	346:349	arg1	environment					351:361	the oral environment	342:361	the oral environment	342:361	When added to the traditional denture material to form a resin base, it could promote good oral health by improving the oral environment.
26718869	4	13	from	months	492:497	arg1	saliva					526:531	artificial saliva	515:531	artificial saliva	515:531	After three months of immersion in artificial saliva, the specimens were tested for tensile strength and were scanned by electron microscope.
26718869	6	14	theme	fibroblast	813:822	arg1	cells					824:828	murine L929 fibroblast cells	801:828	murine L929 fibroblast cells	801:828	The result showed no significant differences in the tensile strength and in the proliferation of murine L929 fibroblast cells.
26718869	7	15	theme	denture	896:902	arg1	material					904:911	chitosan quaternary ammonium salt-modified denture material	853:911	chitosan quaternary ammonium salt-modified denture material	853:911	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	1	16	theme	appreciable	193:203	arg1	solubility					205:214	appreciable solubility	193:214	appreciable solubility in water	193:223	Chitosan quaternary ammonium salt displays good antioxidant and antibacterial characteristics and it shows appreciable solubility in water.
26718869	5	17	theme	murine	626:631	arg1	cytotoxicity					644:655	The murine fibroblast cytotoxicity	622:655	The murine fibroblast cytotoxicity	622:655	The murine fibroblast cytotoxicity and antibacterial properties were also tested.
26718869	6	18	theme	L929	808:811	arg1	cells					824:828	murine L929 fibroblast cells	801:828	murine L929 fibroblast cells	801:828	The result showed no significant differences in the tensile strength and in the proliferation of murine L929 fibroblast cells.
26718869	1	19	theme	Chitosan	86:93	arg1	ammonium					106:113	Chitosan quaternary ammonium	86:113	Chitosan quaternary ammonium salt	86:118	Chitosan quaternary ammonium salt displays good antioxidant and antibacterial characteristics and it shows appreciable solubility in water.
26718869	7	20	theme	quaternary	862:871	arg1	material					904:911	chitosan quaternary ammonium salt-modified denture material	853:911	chitosan quaternary ammonium salt-modified denture material	853:911	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	3	21	theme	chitosan	379:386	arg1	ammonium					399:406	chitosan quaternary ammonium	379:406	chitosan quaternary ammonium salt	379:411	In this study, chitosan quaternary ammonium salt was added to the denture material following two different methods.
26718869	5	22	theme	fibroblast	633:642	arg1	cytotoxicity					644:655	The murine fibroblast cytotoxicity	622:655	The murine fibroblast cytotoxicity	622:655	The murine fibroblast cytotoxicity and antibacterial properties were also tested.
26718869	6	23	theme	murine	801:806	arg1	cells					824:828	murine L929 fibroblast cells	801:828	murine L929 fibroblast cells	801:828	The result showed no significant differences in the tensile strength and in the proliferation of murine L929 fibroblast cells.
26718869	1	24	theme	quaternary	95:104	arg1	ammonium					106:113	Chitosan quaternary ammonium	86:113	Chitosan quaternary ammonium salt	86:118	Chitosan quaternary ammonium salt displays good antioxidant and antibacterial characteristics and it shows appreciable solubility in water.
26718869	7	25	theme	ammonium	873:880	arg1	material					904:911	chitosan quaternary ammonium salt-modified denture material	853:911	chitosan quaternary ammonium salt-modified denture material	853:911	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	3	26	theme	quaternary	388:397	arg1	ammonium					399:406	chitosan quaternary ammonium	379:406	chitosan quaternary ammonium salt	379:411	In this study, chitosan quaternary ammonium salt was added to the denture material following two different methods.
26718869	8	27	theme	generation	1122:1131	arg1	material					1156:1163	a new generation oral denture composite material	1116:1163	a new generation oral denture composite material	1116:1163	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	7	28	theme	antimicrobial	963:975	arg1	properties					977:986	antimicrobial properties	963:986	antimicrobial properties	963:986	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	2	29	theme	oral	317:320	arg1	health					322:327	good oral health	312:327	good oral health	312:327	When added to the traditional denture material to form a resin base, it could promote good oral health by improving the oral environment.
26718869	0	30	theme	quaternary	23:32	arg1	ammonium					34:41	chitosan quaternary ammonium	14:41	chitosan quaternary ammonium	14:41	Evaluation of chitosan quaternary ammonium salt-modified resin denture base material.
26718869	8	31	theme	composite	1146:1154	arg1	material					1156:1163	a new generation oral denture composite material	1116:1163	a new generation oral denture composite material	1116:1163	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	7	32	theme	properties	977:986	arg1	degrees					927:933	different degrees	917:933	different degrees of corrosion resistance and antimicrobial properties	917:986	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	2	33	theme	good	312:315	arg1	health					322:327	good oral health	312:327	good oral health	312:327	When added to the traditional denture material to form a resin base, it could promote good oral health by improving the oral environment.
26718869	7	34	theme	chitosan	853:860	arg1	material					904:911	chitosan quaternary ammonium salt-modified denture material	853:911	chitosan quaternary ammonium salt-modified denture material	853:911	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	3	35	theme	different	461:469	arg1	methods					471:477	two different methods	457:477	two different methods	457:477	In this study, chitosan quaternary ammonium salt was added to the denture material following two different methods.
26718869	1	36	theme	ammonium	106:113	arg1	salt					115:118	Chitosan quaternary ammonium salt	86:118	Chitosan quaternary ammonium salt	86:118	Chitosan quaternary ammonium salt displays good antioxidant and antibacterial characteristics and it shows appreciable solubility in water.
26718869	4	37	theme	tensile	564:570	arg1	strength					572:579	tensile strength	564:579	tensile strength	564:579	After three months of immersion in artificial saliva, the specimens were tested for tensile strength and were scanned by electron microscope.
26718869	8	38	theme	denture	1138:1144	arg1	material					1156:1163	a new generation oral denture composite material	1116:1163	a new generation oral denture composite material	1116:1163	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	8	39	theme	new	1118:1120	arg1	material					1156:1163	a new generation oral denture composite material	1116:1163	a new generation oral denture composite material	1116:1163	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	2	40	dep	material	264:271	arg1	form					276:279	form	276:279	to form a resin base	273:292	When added to the traditional denture material to form a resin base, it could promote good oral health by improving the oral environment.
26718869	0	41	theme	ammonium	34:41	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of chitosan quaternary ammonium	0:41	Evaluation of chitosan quaternary ammonium salt-modified resin denture base material.
26718869	2	42	theme	denture	256:262	arg1	material					264:271	the traditional denture material	240:271	the traditional denture material to form a resin base	240:292	When added to the traditional denture material to form a resin base, it could promote good oral health by improving the oral environment.
26718869	7	43	theme	salt-modified	882:894	arg1	material					904:911	chitosan quaternary ammonium salt-modified denture material	853:911	chitosan quaternary ammonium salt-modified denture material	853:911	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	0	44	theme	denture	63:69	arg1	material					76:83	resin denture base material	57:83	resin denture base material	57:83	Evaluation of chitosan quaternary ammonium salt-modified resin denture base material.
26718869	3	45	theme	denture	430:436	arg1	material					438:445	the denture material	426:445	the denture material following two different methods	426:477	In this study, chitosan quaternary ammonium salt was added to the denture material following two different methods.
26718869	2	46	theme	traditional	244:254	arg1	material					264:271	the traditional denture material	240:271	the traditional denture material to form a resin base	240:292	When added to the traditional denture material to form a resin base, it could promote good oral health by improving the oral environment.
26718869	1	47	theme	good	129:132	arg1	characteristics					164:178	good antioxidant and antibacterial characteristics	129:178	good antioxidant and antibacterial characteristics	129:178	Chitosan quaternary ammonium salt displays good antioxidant and antibacterial characteristics and it shows appreciable solubility in water.
26718869	0	48	theme	resin	57:61	arg1	material					76:83	resin denture base material	57:83	resin denture base material	57:83	Evaluation of chitosan quaternary ammonium salt-modified resin denture base material.
26718869	1	49	theme	antioxidant	134:144	arg1	characteristics					164:178	good antioxidant and antibacterial characteristics	129:178	good antioxidant and antibacterial characteristics	129:178	Chitosan quaternary ammonium salt displays good antioxidant and antibacterial characteristics and it shows appreciable solubility in water.
26718869	7	50	theme	resistance	948:957	arg1	degrees					927:933	different degrees	917:933	different degrees of corrosion resistance and antimicrobial properties	917:986	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	8	51	theme	base	1074:1077	arg1	material					1079:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	2	52	theme	resin	283:287	arg1	base					289:292	a resin base	281:292	a resin base	281:292	When added to the traditional denture material to form a resin base, it could promote good oral health by improving the oral environment.
26718869	8	53	theme	quaternary	1026:1035	arg1	material					1079:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	8	54	theme	salt-modified	1046:1058	arg1	material					1079:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	4	55	theme	electron	601:608	arg1	microscope					610:619	electron microscope	601:619	electron microscope	601:619	After three months of immersion in artificial saliva, the specimens were tested for tensile strength and were scanned by electron microscope.
26718869	6	56	theme	tensile	756:762	arg1	strength					764:771	the tensile strength	752:771	the tensile strength	752:771	The result showed no significant differences in the tensile strength and in the proliferation of murine L929 fibroblast cells.
26718869	1	57	theme	antibacterial	150:162	arg1	characteristics					164:178	good antioxidant and antibacterial characteristics	129:178	good antioxidant and antibacterial characteristics	129:178	Chitosan quaternary ammonium salt displays good antioxidant and antibacterial characteristics and it shows appreciable solubility in water.
26718869	8	58	contain	has	1088:1090	arg1	material					1079:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	8	58	contain	has	1088:1090	arg2	potential					1096:1104	the potential to become a new generation oral denture composite material	1092:1163	the potential to become a new generation oral denture composite material	1092:1163	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	7	59	theme	corrosion	938:946	arg1	resistance					948:957	corrosion resistance	938:957	corrosion resistance	938:957	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
26718869	0	60	theme	base	71:74	arg1	material					76:83	resin denture base material	57:83	resin denture base material	57:83	Evaluation of chitosan quaternary ammonium salt-modified resin denture base material.
26718869	4	61	theme	immersion	502:510	arg1	months					492:497	three months	486:497	three months of immersion in artificial saliva	486:531	After three months of immersion in artificial saliva, the specimens were tested for tensile strength and were scanned by electron microscope.
26718869	0	62	theme	chitosan	14:21	arg1	ammonium					34:41	chitosan quaternary ammonium	14:41	chitosan quaternary ammonium	14:41	Evaluation of chitosan quaternary ammonium salt-modified resin denture base material.
26718869	8	63	theme	chitosan	1017:1024	arg1	material					1079:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	chitosan quaternary ammonium salt-modified resin denture base material	1017:1086	These results indicate that chitosan quaternary ammonium salt-modified resin denture base material has the potential to become a new generation oral denture composite material.
26718869	7	64	theme	different	917:925	arg1	degrees					927:933	different degrees	917:933	different degrees of corrosion resistance and antimicrobial properties	917:986	The two structures of chitosan quaternary ammonium salt-modified denture material had different degrees of corrosion resistance and antimicrobial properties.
24846282	2	0	theme	bacterial	473:481	arg1	community					483:491	the bacterial community	469:491	the bacterial community	469:491	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	7	1	theme	caecal	1566:1571	arg1	populations					1583:1593	caecal bacterial populations	1566:1593	caecal bacterial populations	1566:1593	Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.
24846282	2	2	from	changes	439:445	arg1	caecum					523:528	the caecum	519:528	the caecum	519:528	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	2	2	from	changes	439:445	arg1	composition					454:464	the composition	450:464	the composition of the bacterial community	450:491	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	2	2	from	changes	439:445	arg1	morphology					505:514	mucosal morphology	497:514	mucosal morphology	497:514	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	3	3	theme	feeding	548:554	arg1	weeks					539:543	7 weeks	537:543	7 weeks of feeding	537:554	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	4	4	dep	P=	816:817	arg1	P=					828:829	P= 0·009	828:835	P= 0·001 to P= 0·009	816:835	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	4	4	dep	P=	816:817	arg1	to					825:826	to	825:826	to	825:826	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	5	5	theme	caecal	1286:1291	arg1	mucosa					1293:1298	the caecal mucosa	1282:1298	the caecal mucosa	1282:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	4	6	theme	hay	1078:1080	arg1	diet					1082:1085	the hay diet	1074:1085	the hay diet	1074:1085	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	4	7	dep	increased	805:813	arg1	P=					816:817	P= 0·001 to P= 0·009	816:835	P= 0·001 to P= 0·009	816:835	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	2	8	theme	present	273:279	arg1	study					281:285	the present study	269:285	the present study	269:285	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	1	9	theme	feeding	160:166	arg1	effect					134:139	The effect	130:139	The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health	130:244	The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown.
24846282	1	9	theme	feeding	160:166	arg1	unknown					257:263	unknown	257:263	unknown	257:263	The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown.
24846282	7	10	theme	significant	1755:1765	arg1	role					1767:1770	a significant role	1753:1770	a significant role	1753:1770	Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.
24846282	2	11	theme	male	292:295	arg1	goats					297:301	ten male goats	288:301	ten male goats	288:301	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	1	12	theme	caecal	171:176	arg1	composition					199:209	caecal bacterial microbiota composition	171:209	caecal bacterial microbiota composition	171:209	The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown.
24846282	5	13	from	expression	1221:1230	arg1	mucosa					1293:1298	the caecal mucosa	1282:1298	the caecal mucosa	1282:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	3	14	dep	P<	619:620	arg1	to					628:629	to	628:629	to	628:629	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	3	14	dep	P<	619:620	arg1	P<					631:632	P<	631:632	P<	631:632	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	1	15	theme	microbiota	188:197	arg1	composition					199:209	caecal bacterial microbiota composition	171:209	caecal bacterial microbiota composition	171:209	The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown.
24846282	4	16	theme	caecal	910:915	arg1	lumen					917:921	the caecal lumen	906:921	the caecal lumen	906:921	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	0	17	from	injury	113:118	arg1	goats					123:127	goats	123:127	goats	123:127	High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats.
24846282	6	18	theme	correlation	1305:1315	arg1	analysis					1317:1324	The correlation analysis	1301:1324	The correlation analysis	1301:1324	The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation.
24846282	6	19	from	alterations	1340:1350	arg1	abundance					1417:1425	mucosa-associated microbiota abundance	1388:1425	mucosa-associated microbiota abundance	1388:1425	The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation.
24846282	6	19	from	alterations	1340:1350	arg1	pH					1362:1363	caecal pH	1355:1363	caecal pH	1355:1363	The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation.
24846282	6	19	from	alterations	1340:1350	arg1	concentration					1370:1382	LPS concentration	1366:1382	LPS concentration	1366:1382	The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation.
24846282	6	20	theme	microbiota	1406:1415	arg1	abundance					1417:1425	mucosa-associated microbiota abundance	1388:1425	mucosa-associated microbiota abundance	1388:1425	The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation.
24846282	3	21	theme	digesta	652:658	arg1	concentrations					660:673	the caecal digesta concentrations	641:673	the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS)	641:732	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	1	22	theme	high-grain	144:153	arg1	feeding					160:166	high-grain (HG) feeding	144:166	high-grain (HG) feeding	144:166	The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown.
24846282	3	23	theme	acids	699:703	arg1	concentrations					660:673	the caecal digesta concentrations	641:673	the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS)	641:732	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	1	24	theme	HG	156:157	arg1	feeding					160:166	high-grain (HG) feeding	144:166	high-grain (HG) feeding	144:166	The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown.
24846282	3	25	theme	volatile	684:691	arg1	acids					699:703	total volatile fatty acids	678:703	total volatile fatty acids	678:703	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	4	26	theme	ribosomal	761:769	arg1	gene					775:778	the 16S ribosomal RNA gene	753:778	the 16S ribosomal RNA gene	753:778	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	0	27	theme	caecal	98:103	arg1	injury					113:118	caecal mucosal injury	98:118	caecal mucosal injury in goats	98:127	High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats.
24846282	2	28	theme	grain	407:411	arg1	diet					396:399	a HG diet	391:399	a HG diet (65 % grain; n 5)	391:417	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	2	28	theme	grain	407:411	arg1	n					414:414	65 % grain; n 5	402:416	65 % grain; n 5	402:416	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	3	29	dep	increased	608:616	arg1	P<					619:620	P<	619:620	P<	619:620	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	6	30	theme	caecal	1355:1360	arg1	pH					1362:1363	caecal pH	1355:1363	caecal pH	1355:1363	The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation.
24846282	3	31	theme	lipopolysaccharide	709:726	arg1	concentrations					660:673	the caecal digesta concentrations	641:673	the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS)	641:732	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	5	32	theme	mRNA	1216:1219	arg1	expression					1221:1230	the relative mRNA expression	1203:1230	the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa	1203:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	5	33	theme	HG	1105:1106	arg1	goats					1117:1121	the HG diet-fed goats	1101:1121	the HG diet-fed goats	1101:1121	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	2	34	theme	hay	350:352	arg1	diet					354:357	a hay diet	348:357	a hay diet (0 % grain; n 5)	348:374	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	2	34	theme	hay	350:352	arg1	n					371:371	0 % grain; n 5	360:373	0 % grain; n 5	360:373	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	0	35	theme	High-grain	0:9	arg1	feeding					11:17	High-grain feeding	0:17	High-grain feeding	0:17	High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats.
24846282	5	36	from	mucosa	1293:1298	arg1	damage					1152:1157	intense epithelial damage	1133:1157	intense epithelial damage	1133:1157	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	5	36	from	mucosa	1293:1298	arg1	P<					1178:1179	P<	1178:1179	P<	1178:1179	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	5	36	from	mucosa	1293:1298	arg1	up-regulation					1163:1175	up-regulation	1163:1175	up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa	1163:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	5	37	from	up-regulation	1163:1175	arg1	mucosa					1293:1298	the caecal mucosa	1282:1298	the caecal mucosa	1282:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	2	38	theme	%	362:362	arg1	diet					354:357	a hay diet	348:357	a hay diet (0 % grain; n 5)	348:374	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	2	38	theme	%	362:362	arg1	n					371:371	0 % grain; n 5	360:373	0 % grain; n 5	360:373	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	0	39	theme	bacterial	33:41	arg1	composition					54:64	caecal bacterial microbiota composition	26:64	caecal bacterial microbiota composition	26:64	High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats.
24846282	7	40	theme	fermentable	1638:1648	arg1	substrates					1650:1659	the fermentable substrates	1634:1659	the fermentable substrates that flow into the caecum	1634:1685	Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.
24846282	5	41	from	damage	1152:1157	arg1	mucosa					1293:1298	the caecal mucosa	1282:1298	the caecal mucosa	1282:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	4	42	theme	Turicibacter	874:885	arg1	abundance					842:850	the abundance	838:850	the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa	838:946	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	7	43	theme	bacterial	1573:1581	arg1	populations					1583:1593	caecal bacterial populations	1566:1593	caecal bacterial populations	1566:1593	Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.
24846282	4	44	theme	Clostridium	891:901	arg1	abundance					842:850	the abundance	838:850	the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa	838:946	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	4	45	theme	Mucispirillum	1032:1044	arg1	proportion					989:998	the proportion	985:998	the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa	985:1058	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	4	46	from	mucosa	941:946	arg1	abundance					842:850	the abundance	838:850	the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa	838:946	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	3	47	theme	HG	561:562	arg1	diet					564:567	the HG diet	557:567	the HG diet	557:567	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	5	48	theme	intense	1133:1139	arg1	damage					1152:1157	intense epithelial damage	1133:1157	intense epithelial damage	1133:1157	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	7	49	theme	adaptive	1545:1552	arg1	response					1554:1561	the adaptive response	1541:1561	the adaptive response of caecal bacterial populations to HG feeding in goats	1541:1616	Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.
24846282	4	50	theme	predominant	855:865	arg1	Turicibacter					874:885	predominant genera Turicibacter	855:885	predominant genera Turicibacter	855:885	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	3	51	theme	caecal	583:588	arg1	pH					590:591	the caecal pH	579:591	the caecal pH	579:591	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	4	52	dep	decreased	952:960	arg1	P<					963:964	P<	963:964	P<	963:964	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	7	53	theme	caecal	1775:1780	arg1	dysfunction					1782:1792	caecal dysfunction	1775:1792	caecal dysfunction	1775:1792	Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.
24846282	2	54	theme	mucosal	497:503	arg1	morphology					505:514	mucosal morphology	497:514	mucosal morphology	497:514	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	4	55	theme	caecal	934:939	arg1	mucosa					941:946	the caecal mucosa	930:946	the caecal mucosa	930:946	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	4	56	theme	HG	794:795	arg1	feeding					797:803	HG feeding	794:803	HG feeding	794:803	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	2	57	theme	community	483:491	arg1	composition					454:464	the composition	450:464	the composition of the bacterial community	450:491	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	2	57	theme	community	483:491	arg1	morphology					505:514	mucosal morphology	497:514	mucosal morphology	497:514	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	1	58	theme	bacterial	178:186	arg1	composition					199:209	caecal bacterial microbiota composition	171:209	caecal bacterial microbiota composition	171:209	The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown.
24846282	4	59	dep	P<	963:964	arg1	to					972:973	to	972:973	to	972:973	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	4	59	dep	P<	963:964	arg1	P<					975:976	P<	975:976	P<	975:976	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	1	60	dep	fermentation	215:226	arg1	health					239:244	health	239:244	health	239:244	The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown.
24846282	4	61	from	Clostridium	891:901	arg1	mucosa					941:946	the caecal mucosa	930:946	the caecal mucosa	930:946	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	4	61	from	Clostridium	891:901	arg1	lumen					917:921	the caecal lumen	906:921	the caecal lumen	906:921	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	7	62	theme	microbial	1721:1729	arg1	compositions					1731:1742	microbial compositions	1721:1742	microbial compositions	1721:1742	Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.
24846282	4	63	from	Turicibacter	874:885	arg1	mucosa					941:946	the caecal mucosa	930:946	the caecal mucosa	930:946	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	4	63	from	Turicibacter	874:885	arg1	lumen					917:921	the caecal lumen	906:921	the caecal lumen	906:921	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	3	64	theme	total	678:682	arg1	acids					699:703	total volatile fatty acids	678:703	total volatile fatty acids	678:703	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	6	65	theme	mucosa-associated	1388:1404	arg1	abundance					1417:1425	mucosa-associated microbiota abundance	1388:1425	mucosa-associated microbiota abundance	1388:1425	The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation.
24846282	5	66	dep	P<	1178:1179	arg1	to					1187:1188	to	1187:1188	to	1187:1188	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	5	66	dep	P<	1178:1179	arg1	P<					1190:1191	P<	1190:1191	P<	1190:1191	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	4	67	from	lumen	917:921	arg1	abundance					842:850	the abundance	838:850	the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa	838:946	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	7	68	theme	dramatic	1697:1704	arg1	alterations					1706:1716	dramatic alterations	1697:1716	dramatic alterations	1697:1716	Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.
24846282	4	69	from	lumen	1022:1026	arg1	proportion					989:998	the proportion	985:998	the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa	985:1058	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	4	70	from	abundance	842:850	arg1	mucosa					941:946	the caecal mucosa	930:946	the caecal mucosa	930:946	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	4	70	from	abundance	842:850	arg1	lumen					917:921	the caecal lumen	906:921	the caecal lumen	906:921	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	2	71	theme	HG	393:394	arg1	diet					396:399	a HG diet	391:399	a HG diet (65 % grain; n 5)	391:417	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	2	71	theme	HG	393:394	arg1	n					414:414	65 % grain; n 5	402:416	65 % grain; n 5	402:416	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	5	72	theme	IL-1β	1235:1239	arg1	expression					1221:1230	the relative mRNA expression	1203:1230	the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa	1203:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	4	73	theme	Bacteroides	1003:1013	arg1	proportion					989:998	the proportion	985:998	the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa	985:1058	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	3	74	theme	caecal	645:650	arg1	concentrations					660:673	the caecal digesta concentrations	641:673	the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS)	641:732	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	0	75	theme	mucosal	105:111	arg1	injury					113:118	caecal mucosal injury	98:118	caecal mucosal injury in goats	98:127	High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats.
24846282	6	76	theme	LPS	1366:1368	arg1	concentration					1370:1382	LPS concentration	1366:1382	LPS concentration	1366:1382	The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation.
24846282	3	77	theme	fatty	693:697	arg1	acids					699:703	total volatile fatty acids	678:703	total volatile fatty acids	678:703	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	4	78	theme	16S	757:759	arg1	gene					775:778	the 16S ribosomal RNA gene	753:778	the 16S ribosomal RNA gene	753:778	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	6	79	theme	local	1472:1476	arg1	inflammation					1478:1489	local inflammation	1472:1489	local inflammation	1472:1489	The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation.
24846282	5	80	theme	IL-6	1242:1245	arg1	expression					1221:1230	the relative mRNA expression	1203:1230	the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa	1203:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	2	81	theme	%	405:405	arg1	diet					396:399	a HG diet	391:399	a HG diet (65 % grain; n 5)	391:417	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	2	81	theme	%	405:405	arg1	n					414:414	65 % grain; n 5	402:416	65 % grain; n 5	402:416	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	5	82	theme	relative	1207:1214	arg1	expression					1221:1230	the relative mRNA expression	1203:1230	the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa	1203:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	4	83	theme	gene	775:778	arg1	Pyrosequencing					735:748	Pyrosequencing	735:748	Pyrosequencing of the 16S ribosomal RNA gene	735:778	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	1	84	from	effect	134:139	arg1	fermentation					215:226	fermentation	215:226	fermentation	215:226	The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown.
24846282	1	84	from	effect	134:139	arg1	composition					199:209	caecal bacterial microbiota composition	171:209	caecal bacterial microbiota composition	171:209	The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown.
24846282	4	85	from	proportion	989:998	arg1	mucosa					1053:1058	the mucosa	1049:1058	the mucosa	1049:1058	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	4	85	from	proportion	989:998	arg1	lumen					1022:1026	the lumen	1018:1026	the lumen	1018:1026	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	5	86	theme	expression	1221:1230	arg1	damage					1152:1157	intense epithelial damage	1133:1157	intense epithelial damage	1133:1157	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	5	86	theme	expression	1221:1230	arg1	P<					1178:1179	P<	1178:1179	P<	1178:1179	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	5	86	theme	expression	1221:1230	arg1	up-regulation					1163:1175	up-regulation	1163:1175	up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa	1163:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	0	87	theme	caecal	26:31	arg1	composition					54:64	caecal bacterial microbiota composition	26:64	caecal bacterial microbiota composition	26:64	High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats.
24846282	2	88	theme	grain	364:368	arg1	diet					354:357	a hay diet	348:357	a hay diet (0 % grain; n 5)	348:374	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	2	88	theme	grain	364:368	arg1	n					371:371	0 % grain; n 5	360:373	0 % grain; n 5	360:373	In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum.
24846282	0	89	theme	microbiota	43:52	arg1	composition					54:64	caecal bacterial microbiota composition	26:64	caecal bacterial microbiota composition	26:64	High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats.
24846282	7	90	from	feeding	1601:1607	arg1	goats					1612:1616	goats	1612:1616	goats	1612:1616	Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.
24846282	5	91	theme	epithelial	1141:1150	arg1	damage					1152:1157	intense epithelial damage	1133:1157	intense epithelial damage	1133:1157	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	4	92	theme	RNA	771:773	arg1	gene					775:778	the 16S ribosomal RNA gene	753:778	the 16S ribosomal RNA gene	753:778	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	3	93	dep	decreased	569:577	arg1	P<					594:595	P< 0·001	594:601	P< 0·001	594:601	After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS).
24846282	5	94	theme	IL-12	1248:1252	arg1	expression					1221:1230	the relative mRNA expression	1203:1230	the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa	1203:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	6	95	theme	HG	1434:1435	arg1	feeding					1437:1443	HG feeding	1434:1443	HG feeding	1434:1443	The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation.
24846282	7	96	theme	HG	1598:1599	arg1	feeding					1601:1607	HG feeding	1598:1607	HG feeding in goats	1598:1616	Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.
24846282	4	97	theme	genera	867:872	arg1	Turicibacter					874:885	predominant genera Turicibacter	855:885	predominant genera Turicibacter	855:885	Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet.
24846282	5	98	theme	interferon-γ	1258:1269	arg1	expression					1221:1230	the relative mRNA expression	1203:1230	the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa	1203:1298	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	5	99	theme	diet-fed	1108:1115	arg1	goats					1117:1121	the HG diet-fed goats	1101:1121	the HG diet-fed goats	1101:1121	Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa.
24846282	7	100	theme	populations	1583:1593	arg1	response					1554:1561	the adaptive response	1541:1561	the adaptive response of caecal bacterial populations to HG feeding in goats	1541:1616	Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.
25213818	0	0	theme	alginate	98:105	arg1	matrix					117:122	carbon nanosphere/sodium alginate composite matrix	73:122	carbon nanosphere/sodium alginate composite matrix for enzyme immobilization	73:148	Development of highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization.
25213818	3	1	theme	electrochemical	504:518	arg1	spectroscopy					530:541	electrochemical impedance spectroscopy	504:541	electrochemical impedance spectroscopy	504:541	This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase.
25213818	0	2	theme	nanosphere/sodium	80:96	arg1	matrix					117:122	carbon nanosphere/sodium alginate composite matrix	73:122	carbon nanosphere/sodium alginate composite matrix for enzyme immobilization	73:148	Development of highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization.
25213818	1	3	theme	composite	336:344	arg1	matrix					346:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	5	4	from	×	928:928	arg1	range					913:917	a wide linear concentration range	885:917	a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3	885:1024	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	3	5	theme	impedance	520:528	arg1	spectroscopy					530:541	electrochemical impedance spectroscopy	504:541	electrochemical impedance spectroscopy	504:541	This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase.
25213818	0	6	theme	composite	107:115	arg1	matrix					117:122	carbon nanosphere/sodium alginate composite matrix	73:122	carbon nanosphere/sodium alginate composite matrix for enzyme immobilization	73:148	Development of highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization.
25213818	4	7	theme	Various	648:654	arg1	conditions					669:678	Various experimental conditions	648:678	Various experimental conditions	648:678	Various experimental conditions were investigated that influenced the performance of the biosensor, such as pH, applied potential and temperature.
25213818	4	8	theme	biosensor	737:745	arg1	performance					718:728	the performance	714:728	the performance of the biosensor	714:745	Various experimental conditions were investigated that influenced the performance of the biosensor, such as pH, applied potential and temperature.
25213818	1	9	theme	novel	285:289	arg1	matrix					346:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	0	10	theme	enzyme	128:133	arg1	immobilization					135:148	enzyme immobilization	128:148	enzyme immobilization	128:148	Development of highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization.
25213818	4	11	theme	applied	760:766	arg1	potential					768:776	applied potential	760:776	applied potential	760:776	Various experimental conditions were investigated that influenced the performance of the biosensor, such as pH, applied potential and temperature.
25213818	5	12	theme	concentration	899:911	arg1	range					913:917	a wide linear concentration range	885:917	a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3	885:1024	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	5	13	theme	wide	887:890	arg1	range					913:917	a wide linear concentration range	885:917	a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3	885:1024	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	5	14	theme	optimum	805:811	arg1	conditions					813:822	the optimum conditions	801:822	the optimum conditions	801:822	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	3	15	theme	composite	438:446	arg1	film					448:451	This composite film	433:451	This composite film	433:451	This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase.
25213818	3	16	theme	hybrid	580:585	arg1	suitable					600:607	suitable	600:607	suitable	600:607	This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase.
25213818	3	16	theme	hybrid	580:585	arg1	material					587:594	the hybrid material	576:594	the hybrid material	576:594	This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase.
25213818	1	17	theme	sensitive	188:196	arg1	biosensor					211:219	a highly sensitive amperometric biosensor	179:219	a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	179:351	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	1	18	theme	carbon	291:296	arg1	matrix					346:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	5	19	theme	×	944:944	arg1	M					953:953	4.6 × 10(-3) M	940:953	4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3	940:1024	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	1	20	theme	amperometric	198:209	arg1	biosensor					211:219	a highly sensitive amperometric biosensor	179:219	a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	179:351	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	0	21	theme	sensitive	22:30	arg1	biosensor					45:53	highly sensitive amperometric biosensor	15:53	highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization	15:148	Development of highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization.
25213818	1	22	theme	nanosphere	298:307	arg1	matrix					346:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	2	23	theme	biopolymer	418:427	arg1	SA					429:430	natural biopolymer SA	410:430	natural biopolymer SA	410:430	This hybrid material combined the advantages of CNS and natural biopolymer SA.
25213818	2	24	theme	SA	429:430	arg1	advantages					388:397	the advantages	384:397	the advantages of CNS and natural biopolymer SA	384:430	This hybrid material combined the advantages of CNS and natural biopolymer SA.
25213818	5	25	theme	3	1024:1024	arg1	ratio					1015:1019	a signal-to-noise ratio	997:1019	a signal-to-noise ratio of 3	997:1024	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	5	26	theme	excellent	846:854	arg1	performance					856:866	excellent performance	846:866	excellent performance for glucose	846:878	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	2	27	theme	natural	410:416	arg1	SA					429:430	natural biopolymer SA	410:430	natural biopolymer SA	410:430	This hybrid material combined the advantages of CNS and natural biopolymer SA.
25213818	0	28	theme	biosensor	45:53	arg1	Development					0:10	Development	0:10	Development of highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization.	0:149	Development of highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization.
25213818	1	29	theme	CNS	310:312	arg1	matrix					346:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	1	30	theme	glucose	225:231	arg1	detection					233:241	glucose detection	225:241	glucose detection	225:241	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	0	31	theme	amperometric	32:43	arg1	biosensor					45:53	highly sensitive amperometric biosensor	15:53	highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization	15:148	Development of highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization.
25213818	5	32	with	M	953:953	arg1	limit					972:976	a detection limit	960:976	a detection limit of 0.5 μM based on a signal-to-noise ratio of 3	960:1024	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	5	33	theme	μM	985:986	arg1	limit					972:976	a detection limit	960:976	a detection limit of 0.5 μM based on a signal-to-noise ratio of 3	960:1024	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	2	34	theme	CNS	402:404	arg1	advantages					388:397	the advantages	384:397	the advantages of CNS and natural biopolymer SA	384:430	This hybrid material combined the advantages of CNS and natural biopolymer SA.
25213818	1	35	theme	/sodium	314:320	arg1	matrix					346:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	6	36	theme	satisfactory	1098:1109	arg1	reproducibility					1111:1125	satisfactory reproducibility	1098:1125	satisfactory reproducibility	1098:1125	Furthermore, the biosensor exhibited excellent long-term stability and satisfactory reproducibility.
25213818	1	37	theme	alginate	322:329	arg1	matrix					346:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	5	38	theme	signal-to-noise	999:1013	arg1	ratio					1015:1019	a signal-to-noise ratio	997:1019	a signal-to-noise ratio of 3	997:1024	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	3	39	theme	oxidase	639:645	arg1	immobilization					613:626	immobilization	613:626	immobilization of glucose oxidase	613:645	This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase.
25213818	3	40	theme	scanning	474:481	arg1	microscope					492:501	scanning electron microscope	474:501	scanning electron microscope	474:501	This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase.
25213818	6	41	theme	long-term	1074:1082	arg1	stability					1084:1092	excellent long-term stability	1064:1092	excellent long-term stability	1064:1092	Furthermore, the biosensor exhibited excellent long-term stability and satisfactory reproducibility.
25213818	5	42	theme	detection	962:970	arg1	limit					972:976	a detection limit	960:976	a detection limit of 0.5 μM based on a signal-to-noise ratio of 3	960:1024	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	3	43	theme	electron	483:490	arg1	microscope					492:501	scanning electron microscope	474:501	scanning electron microscope	474:501	This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase.
25213818	0	44	theme	carbon	73:78	arg1	matrix					117:122	carbon nanosphere/sodium alginate composite matrix	73:122	carbon nanosphere/sodium alginate composite matrix for enzyme immobilization	73:148	Development of highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization.
25213818	4	45	theme	experimental	656:667	arg1	conditions					669:678	Various experimental conditions	648:678	Various experimental conditions	648:678	Various experimental conditions were investigated that influenced the performance of the biosensor, such as pH, applied potential and temperature.
25213818	6	46	theme	excellent	1064:1072	arg1	stability					1084:1092	excellent long-term stability	1064:1092	excellent long-term stability	1064:1092	Furthermore, the biosensor exhibited excellent long-term stability and satisfactory reproducibility.
25213818	5	47	theme	linear	892:897	arg1	range					913:917	a wide linear concentration range	885:917	a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3	885:1024	Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3.
25213818	1	48	theme	glucose	252:258	arg1	oxidase					260:266	glucose oxidase	252:266	glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	252:351	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	1	49	theme	SA	332:333	arg1	matrix					346:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix	283:351	In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix.
25213818	3	50	theme	glucose	631:637	arg1	oxidase					639:645	glucose oxidase	631:645	glucose oxidase	631:645	This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase.
25213818	2	51	theme	hybrid	359:364	arg1	material					366:373	This hybrid material	354:373	This hybrid material	354:373	This hybrid material combined the advantages of CNS and natural biopolymer SA.
24524856	1	0	theme	present	156:162	arg1	study					164:168	The present study	152:168	The present study	152:168	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	0	1	from	role	4:7	arg1	fiber					95:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber	23:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution	23:149	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	2	2	theme	composite	341:349	arg1	fiber					351:355	chitosan/biomass composite fiber	324:355	chitosan/biomass composite fiber	324:355	To make PBBF, chitosan/biomass composite fiber was coated with PEI and then cross-linked by glutaraldehyde.
24524856	5	3	theme	chitosan/biomass	684:699	arg1	fiber					711:715	the chitosan/biomass composite fiber	680:715	the chitosan/biomass composite fiber	680:715	Meanwhile, the chitosan/biomass composite fiber could successfully coated with PEI and primary amine groups were significantly increased on the surface of the fiber.
24524856	5	3	theme	chitosan/biomass	684:699	arg1	Meanwhile					669:677	Meanwhile	669:677	Meanwhile	669:677	Meanwhile, the chitosan/biomass composite fiber could successfully coated with PEI and primary amine groups were significantly increased on the surface of the fiber.
24524856	1	4	theme	-coated	267:273	arg1	PEI					263:265	PEI	263:265	PEI	263:265	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	1	4	theme	-coated	267:273	arg1	polyethylenimine					245:260	functionalizing polyethylenimine	229:260	functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF)	229:307	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	5	5	theme	composite	701:709	arg1	fiber					711:715	the chitosan/biomass composite fiber	680:715	the chitosan/biomass composite fiber	680:715	Meanwhile, the chitosan/biomass composite fiber could successfully coated with PEI and primary amine groups were significantly increased on the surface of the fiber.
24524856	5	5	theme	composite	701:709	arg1	Meanwhile					669:677	Meanwhile	669:677	Meanwhile	669:677	Meanwhile, the chitosan/biomass composite fiber could successfully coated with PEI and primary amine groups were significantly increased on the surface of the fiber.
24524856	3	6	theme	sorption	476:483	arg1	analyses					519:526	FTIR and XPS analyses	506:526	analyses	519:526	The role of biomass in the fiber was investigated through sorption experiments and SEM, FTIR and XPS analyses with differently prepared fiber sorbents.
24524856	3	6	theme	sorption	476:483	arg1	experiments					485:495	sorption experiments	476:495	sorption experiments	476:495	The role of biomass in the fiber was investigated through sorption experiments and SEM, FTIR and XPS analyses with differently prepared fiber sorbents.
24524856	0	7	theme	Ru	116:117	arg1	removal					105:111	removal	105:111	removal of Ru from acetic acid waste solution	105:149	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	3	8	theme	XPS	515:517	arg1	analyses					519:526	FTIR and XPS analyses	506:526	analyses	519:526	The role of biomass in the fiber was investigated through sorption experiments and SEM, FTIR and XPS analyses with differently prepared fiber sorbents.
24524856	3	8	theme	XPS	515:517	arg1	experiments					485:495	sorption experiments	476:495	sorption experiments	476:495	The role of biomass in the fiber was investigated through sorption experiments and SEM, FTIR and XPS analyses with differently prepared fiber sorbents.
24524856	3	9	theme	biomass	430:436	arg1	role					422:425	The role	418:425	The role of biomass in the fiber	418:449	The role of biomass in the fiber was investigated through sorption experiments and SEM, FTIR and XPS analyses with differently prepared fiber sorbents.
24524856	3	10	theme	fiber	554:558	arg1	sorbents					560:567	differently prepared fiber sorbents	533:567	differently prepared fiber sorbents	533:567	The role of biomass in the fiber was investigated through sorption experiments and SEM, FTIR and XPS analyses with differently prepared fiber sorbents.
24524856	1	11	theme	bacterial	275:283	arg1	fiber					296:300	bacterial biosorbent fiber	275:300	bacterial biosorbent fiber (PBBF)	275:307	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	1	11	theme	bacterial	275:283	arg1	PBBF					303:306	PBBF	303:306	PBBF	303:306	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	0	12	theme	biomass	12:18	arg1	role					4:7	The role	0:7	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.	0:150	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	1	13	theme	biosorbent	285:294	arg1	fiber					296:300	bacterial biosorbent fiber	275:300	bacterial biosorbent fiber (PBBF)	275:307	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	1	13	theme	biosorbent	285:294	arg1	PBBF					303:306	PBBF	303:306	PBBF	303:306	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	0	14	theme	acid	131:134	arg1	solution					142:149	acetic acid waste solution	124:149	acetic acid waste solution	124:149	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	5	15	theme	primary	756:762	arg1	groups					770:775	primary amine groups	756:775	primary amine groups	756:775	Meanwhile, the chitosan/biomass composite fiber could successfully coated with PEI and primary amine groups were significantly increased on the surface of the fiber.
24524856	0	16	theme	acetic	124:129	arg1	solution					142:149	acetic acid waste solution	124:149	acetic acid waste solution	124:149	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	3	17	theme	prepared	545:552	arg1	sorbents					560:567	differently prepared fiber sorbents	533:567	differently prepared fiber sorbents	533:567	The role of biomass in the fiber was investigated through sorption experiments and SEM, FTIR and XPS analyses with differently prepared fiber sorbents.
24524856	0	18	theme	polyethylenimine-coated	23:45	arg1	fiber					95:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber	23:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution	23:149	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	3	19	with	analyses	519:526	arg1	sorbents					560:567	differently prepared fiber sorbents	533:567	differently prepared fiber sorbents	533:567	The role of biomass in the fiber was investigated through sorption experiments and SEM, FTIR and XPS analyses with differently prepared fiber sorbents.
24524856	0	20	theme	waste	136:140	arg1	solution					142:149	acetic acid waste solution	124:149	acetic acid waste solution	124:149	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	1	21	theme	bacterial	208:216	arg1	biomass					218:224	bacterial biomass	208:224	bacterial biomass	208:224	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	0	22	theme	biomass	66:72	arg1	fiber					95:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber	23:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution	23:149	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	5	23	theme	amine	764:768	arg1	groups					770:775	primary amine groups	756:775	primary amine groups	756:775	Meanwhile, the chitosan/biomass composite fiber could successfully coated with PEI and primary amine groups were significantly increased on the surface of the fiber.
24524856	2	24	theme	chitosan/biomass	324:339	arg1	fiber					351:355	chitosan/biomass composite fiber	324:355	chitosan/biomass composite fiber	324:355	To make PBBF, chitosan/biomass composite fiber was coated with PEI and then cross-linked by glutaraldehyde.
24524856	0	25	theme	chitosan/bacterial	47:64	arg1	fiber					95:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber	23:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution	23:149	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	6	26	theme	chitosan	901:908	arg1	fiber					910:914	PEI-reinforced chitosan fiber	886:914	PEI-reinforced chitosan fiber	886:914	Therefore, the biomass should be essential to make PEI-reinforced chitosan fiber.
24524856	6	27	theme	PEI-reinforced	886:899	arg1	fiber					910:914	PEI-reinforced chitosan fiber	886:914	PEI-reinforced chitosan fiber	886:914	Therefore, the biomass should be essential to make PEI-reinforced chitosan fiber.
24524856	1	28	dep	-coated	267:273	arg1	fiber					296:300	bacterial biosorbent fiber	275:300	bacterial biosorbent fiber (PBBF)	275:307	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	1	28	dep	-coated	267:273	arg1	PBBF					303:306	PBBF	303:306	PBBF	303:306	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	1	29	from	role	200:203	arg1	PEI					263:265	PEI	263:265	PEI	263:265	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	1	29	from	role	200:203	arg1	polyethylenimine					245:260	functionalizing polyethylenimine	229:260	functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF)	229:307	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	0	30	from	solution	142:149	arg1	removal					105:111	removal	105:111	removal of Ru from acetic acid waste solution	105:149	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	3	31	theme	FTIR	506:509	arg1	analyses					519:526	FTIR and XPS analyses	506:526	analyses	519:526	The role of biomass in the fiber was investigated through sorption experiments and SEM, FTIR and XPS analyses with differently prepared fiber sorbents.
24524856	3	31	theme	FTIR	506:509	arg1	experiments					485:495	sorption experiments	476:495	sorption experiments	476:495	The role of biomass in the fiber was investigated through sorption experiments and SEM, FTIR and XPS analyses with differently prepared fiber sorbents.
24524856	0	32	theme	biosorbent	84:93	arg1	fiber					95:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber	23:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution	23:149	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	3	33	from	role	422:425	arg1	fiber					445:449	the fiber	441:449	the fiber	441:449	The role of biomass in the fiber was investigated through sorption experiments and SEM, FTIR and XPS analyses with differently prepared fiber sorbents.
24524856	5	34	theme	fiber	828:832	arg1	surface					813:819	the surface	809:819	the surface of the fiber	809:832	Meanwhile, the chitosan/biomass composite fiber could successfully coated with PEI and primary amine groups were significantly increased on the surface of the fiber.
24524856	1	35	theme	functionalizing	229:243	arg1	PEI					263:265	PEI	263:265	PEI	263:265	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	1	35	theme	functionalizing	229:243	arg1	polyethylenimine					245:260	functionalizing polyethylenimine	229:260	functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF)	229:307	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	0	36	theme	composite	74:82	arg1	fiber					95:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber	23:99	polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution	23:149	The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution.
24524856	1	37	theme	biomass	218:224	arg1	role					200:203	the role	196:203	the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF)	196:307	The present study is aimed at understanding the role of bacterial biomass in functionalizing polyethylenimine (PEI)-coated bacterial biosorbent fiber (PBBF).
24524856	4	38	theme	chitosan	591:598	arg1	fiber					600:604	the chitosan fiber	587:604	the chitosan fiber	587:604	In the case that the chitosan fiber was made without the biomass, it could not be coated with PEI.
26513153	3	0	theme	glucose	789:795	arg1	contents					670:677	higher contents	663:677	higher contents of dry matter, sucrose, total sugars, and total polyacetylenes	663:740	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	0	theme	glucose	789:795	arg1	roots					656:660	larger roots	649:660	larger roots	649:660	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	0	theme	glucose	789:795	arg1	contents					753:760	lower contents	747:760	lower contents of terpenes, fructose, and glucose	747:795	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	2	1	theme	terpenes	431:438	arg1	related					490:496	related	490:496	related	490:496	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	1	theme	terpenes	431:438	arg1	content					380:386	The content	376:386	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size	376:483	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	1	theme	terpenes	431:438	arg1	size					480:483	root size	475:483	root size	475:483	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	1	theme	terpenes	431:438	arg1	quality					463:469	the sensory quality	451:469	the sensory quality	451:469	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	1	2	dep	systems	225:231	arg1	conventional					238:249	conventional	238:249	conventional	238:249	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	1	2	dep	systems	225:231	arg1	systems					269:275	three organic systems	255:275	three organic systems	255:275	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	2	3	theme	root	475:478	arg1	size					480:483	root size	475:483	root size	475:483	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	1	4	theme	chemical	333:340	arg1	composition					342:352	chemical composition	333:352	chemical composition	333:352	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	1	5	theme	eating	128:133	arg1	quality					135:141	The eating quality	124:141	The eating quality of carrots (Daucus carota L.)	124:171	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	2	6	theme	polyacetylenes	411:424	arg1	related					490:496	related	490:496	related	490:496	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	6	theme	polyacetylenes	411:424	arg1	content					380:386	The content	376:386	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size	376:483	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	6	theme	polyacetylenes	411:424	arg1	size					480:483	root size	475:483	root size	475:483	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	6	theme	polyacetylenes	411:424	arg1	quality					463:469	the sensory quality	451:469	the sensory quality	451:469	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	1	7	theme	years	290:294	arg1	impact					206:211	the impact	202:211	the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality	202:373	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	5	8	theme	grown	1005:1009	arg1	quality					979:985	the same eating quality	963:985	the same eating quality	963:985	This result shows that organically grown carrots have the same eating quality as conventionally grown carrots, while being produced in a more sustainable way.
26513153	5	8	theme	grown	1005:1009	arg1	carrots					1011:1017	conventionally grown carrots	990:1017	conventionally grown carrots	990:1017	This result shows that organically grown carrots have the same eating quality as conventionally grown carrots, while being produced in a more sustainable way.
26513153	3	9	theme	total	703:707	arg1	sugars					709:714	total sugars	703:714	total sugars	703:714	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	10	theme	temperature	584:594	arg1	sum					596:598	a higher temperature sum	575:598	a higher temperature sum	575:598	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	11	theme	polyacetylenes	727:740	arg1	contents					670:677	higher contents	663:677	higher contents of dry matter, sucrose, total sugars, and total polyacetylenes	663:740	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	11	theme	polyacetylenes	727:740	arg1	roots					656:660	larger roots	649:660	larger roots	649:660	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	11	theme	polyacetylenes	727:740	arg1	contents					753:760	lower contents	747:760	lower contents of terpenes, fructose, and glucose	747:795	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	1	12	theme	cropping	216:223	arg1	systems					225:231	cropping systems	216:231	cropping systems (one conventional and three organic systems)	216:276	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	0	13	theme	Organic	81:87	arg1	Systems					98:104	Three Organic Cropping Systems	75:104	Three Organic Cropping Systems	75:104	Eating Quality of Carrots (Daucus carota L.) Grown in One Conventional and Three Organic Cropping Systems over Three Years.
26513153	3	14	theme	higher	663:668	arg1	contents					670:677	higher contents	663:677	higher contents of dry matter, sucrose, total sugars, and total polyacetylenes	663:740	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	1	15	theme	sensory	359:365	arg1	quality					367:373	sensory quality	359:373	sensory quality	359:373	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	4	16	theme	conventional	832:843	arg1	carrots					857:863	conventional and organic carrots	832:863	conventional and organic carrots with regard to the investigated parameters	832:906	No differences were found between conventional and organic carrots with regard to the investigated parameters.
26513153	2	17	theme	sugars	403:408	arg1	related					490:496	related	490:496	related	490:496	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	17	theme	sugars	403:408	arg1	content					380:386	The content	376:386	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size	376:483	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	17	theme	sugars	403:408	arg1	size					480:483	root size	475:483	root size	475:483	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	17	theme	sugars	403:408	arg1	quality					463:469	the sensory quality	451:469	the sensory quality	451:469	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	3	18	theme	higher	577:582	arg1	temperature					584:594	a higher temperature	575:594	a higher temperature sum	575:598	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	19	theme	total	721:725	arg1	polyacetylenes					727:740	total polyacetylenes	721:740	total polyacetylenes	721:740	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	1	20	theme	carrots	146:152	arg1	quality					135:141	The eating quality	124:141	The eating quality of carrots (Daucus carota L.)	124:171	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	1	21	theme	growing	282:288	arg1	years					290:294	growing years	282:294	growing years (2007, 2008, and 2009)	282:317	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	1	22	theme	systems	225:231	arg1	impact					206:211	the impact	202:211	the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality	202:373	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	4	23	theme	investigated	884:895	arg1	parameters					897:906	the investigated parameters	880:906	the investigated parameters	880:906	No differences were found between conventional and organic carrots with regard to the investigated parameters.
26513153	0	24	theme	Carrots	18:24	arg1	Quality					7:13	Quality	7:13	Quality of Carrots (Daucus carota L.) Grown in One Conventional and Three Organic Cropping Systems over Three Years	7:121	Eating Quality of Carrots (Daucus carota L.) Grown in One Conventional and Three Organic Cropping Systems over Three Years.
26513153	1	25	dep	years	290:294	arg1	2009					313:316	2009	313:316	2009	313:316	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	1	25	dep	years	290:294	arg1	2008					303:306	2008	303:306	2008	303:306	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	2	26	theme	growing	530:536	arg1	years					538:542	the three growing years	520:542	the three growing years	520:542	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	3	27	from	sum	596:598	arg1	2009					603:606	2009	603:606	2009	603:606	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	0	28	theme	Cropping	89:96	arg1	Systems					98:104	Three Organic Cropping Systems	75:104	Three Organic Cropping Systems	75:104	Eating Quality of Carrots (Daucus carota L.) Grown in One Conventional and Three Organic Cropping Systems over Three Years.
26513153	3	29	theme	lower	747:751	arg1	contents					753:760	lower contents	747:760	lower contents of terpenes, fructose, and glucose	747:795	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	5	30	theme	same	967:970	arg1	quality					979:985	the same eating quality	963:985	the same eating quality	963:985	This result shows that organically grown carrots have the same eating quality as conventionally grown carrots, while being produced in a more sustainable way.
26513153	5	30	theme	same	967:970	arg1	carrots					1011:1017	conventionally grown carrots	990:1017	conventionally grown carrots	990:1017	This result shows that organically grown carrots have the same eating quality as conventionally grown carrots, while being produced in a more sustainable way.
26513153	1	31	from	impact	206:211	arg1	quality					367:373	sensory quality	359:373	sensory quality	359:373	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	1	31	from	impact	206:211	arg1	size					327:330	root size	322:330	root size	322:330	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	1	31	from	impact	206:211	arg1	composition					342:352	chemical composition	333:352	chemical composition	333:352	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	3	32	theme	fructose	775:782	arg1	contents					670:677	higher contents	663:677	higher contents of dry matter, sucrose, total sugars, and total polyacetylenes	663:740	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	32	theme	fructose	775:782	arg1	roots					656:660	larger roots	649:660	larger roots	649:660	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	32	theme	fructose	775:782	arg1	contents					753:760	lower contents	747:760	lower contents of terpenes, fructose, and glucose	747:795	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	33	from	radiation	561:569	arg1	2009					603:606	2009	603:606	2009	603:606	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	5	34	theme	eating	972:977	arg1	quality					979:985	the same eating quality	963:985	the same eating quality	963:985	This result shows that organically grown carrots have the same eating quality as conventionally grown carrots, while being produced in a more sustainable way.
26513153	5	34	theme	eating	972:977	arg1	carrots					1011:1017	conventionally grown carrots	990:1017	conventionally grown carrots	990:1017	This result shows that organically grown carrots have the same eating quality as conventionally grown carrots, while being produced in a more sustainable way.
26513153	2	35	theme	sensory	455:461	arg1	related					490:496	related	490:496	related	490:496	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	35	theme	sensory	455:461	arg1	quality					463:469	the sensory quality	451:469	the sensory quality	451:469	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	5	36	contain	have	958:961	arg1	carrots					950:956	organically grown carrots	932:956	organically grown carrots	932:956	This result shows that organically grown carrots have the same eating quality as conventionally grown carrots, while being produced in a more sustainable way.
26513153	5	36	contain	have	958:961	arg2	carrots					1011:1017	conventionally grown carrots	990:1017	conventionally grown carrots	990:1017	This result shows that organically grown carrots have the same eating quality as conventionally grown carrots, while being produced in a more sustainable way.
26513153	5	36	contain	have	958:961	arg2	quality					979:985	the same eating quality	963:985	the same eating quality	963:985	This result shows that organically grown carrots have the same eating quality as conventionally grown carrots, while being produced in a more sustainable way.
26513153	0	37	dep	Daucus	27:32	arg1	L.					41:42	Daucus carota L.	27:42	Daucus carota L.	27:42	Eating Quality of Carrots (Daucus carota L.) Grown in One Conventional and Three Organic Cropping Systems over Three Years.
26513153	3	38	theme	global	554:559	arg1	radiation					561:569	A higher global radiation	545:569	A higher global radiation	545:569	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	1	39	theme	organic	261:267	arg1	systems					269:275	three organic systems	255:275	three organic systems	255:275	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	3	40	theme	terpenes	765:772	arg1	contents					670:677	higher contents	663:677	higher contents of dry matter, sucrose, total sugars, and total polyacetylenes	663:740	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	40	theme	terpenes	765:772	arg1	roots					656:660	larger roots	649:660	larger roots	649:660	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	40	theme	terpenes	765:772	arg1	contents					753:760	lower contents	747:760	lower contents of terpenes, fructose, and glucose	747:795	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	41	theme	higher	547:552	arg1	radiation					561:569	A higher global radiation	545:569	A higher global radiation	545:569	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	1	42	theme	root	322:325	arg1	size					327:330	root size	322:330	root size	322:330	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	3	43	theme	sucrose	694:700	arg1	contents					670:677	higher contents	663:677	higher contents of dry matter, sucrose, total sugars, and total polyacetylenes	663:740	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	43	theme	sucrose	694:700	arg1	roots					656:660	larger roots	649:660	larger roots	649:660	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	43	theme	sucrose	694:700	arg1	contents					753:760	lower contents	747:760	lower contents of terpenes, fructose, and glucose	747:795	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	44	theme	larger	649:654	arg1	roots					656:660	larger roots	649:660	larger roots	649:660	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	45	theme	dry	682:684	arg1	matter					686:691	dry matter	682:691	dry matter	682:691	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	2	46	theme	matter	395:400	arg1	related					490:496	related	490:496	related	490:496	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	46	theme	matter	395:400	arg1	content					380:386	The content	376:386	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size	376:483	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	46	theme	matter	395:400	arg1	size					480:483	root size	475:483	root size	475:483	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	2	46	theme	matter	395:400	arg1	quality					463:469	the sensory quality	451:469	the sensory quality	451:469	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	1	47	dep	Daucus	155:160	arg1	L.					169:170	Daucus carota L.	155:170	Daucus carota L.	155:170	The eating quality of carrots (Daucus carota L.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality.
26513153	3	48	theme	matter	686:691	arg1	contents					670:677	higher contents	663:677	higher contents of dry matter, sucrose, total sugars, and total polyacetylenes	663:740	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	48	theme	matter	686:691	arg1	roots					656:660	larger roots	649:660	larger roots	649:660	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	48	theme	matter	686:691	arg1	contents					753:760	lower contents	747:760	lower contents of terpenes, fructose, and glucose	747:795	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	5	49	theme	sustainable	1051:1061	arg1	way					1063:1065	a more sustainable way	1044:1065	a more sustainable way	1044:1065	This result shows that organically grown carrots have the same eating quality as conventionally grown carrots, while being produced in a more sustainable way.
26513153	2	50	theme	dry	391:393	arg1	matter					395:400	dry matter	391:400	dry matter	391:400	The content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years.
26513153	5	51	theme	grown	944:948	arg1	carrots					950:956	organically grown carrots	932:956	organically grown carrots	932:956	This result shows that organically grown carrots have the same eating quality as conventionally grown carrots, while being produced in a more sustainable way.
26513153	4	52	theme	organic	849:855	arg1	carrots					857:863	conventional and organic carrots	832:863	conventional and organic carrots with regard to the investigated parameters	832:906	No differences were found between conventional and organic carrots with regard to the investigated parameters.
26513153	3	53	theme	sugars	709:714	arg1	contents					670:677	higher contents	663:677	higher contents of dry matter, sucrose, total sugars, and total polyacetylenes	663:740	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	53	theme	sugars	709:714	arg1	roots					656:660	larger roots	649:660	larger roots	649:660	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
26513153	3	53	theme	sugars	709:714	arg1	contents					753:760	lower contents	747:760	lower contents of terpenes, fructose, and glucose	747:795	A higher global radiation and a higher temperature sum in 2009 as compared to 2007 and 2008 resulted in larger roots, higher contents of dry matter, sucrose, total sugars, and total polyacetylenes, and lower contents of terpenes, fructose, and glucose.
25080396	4	0	theme	release	751:757	arg1	properties					759:768	release properties	751:768	release properties	751:768	The microspheres were characterized for their surface morphology, composition, size distribution, drug loading efficiency and release properties.
25080396	1	1	theme	bone	183:186	arg1	applications					207:218	bone tissue engineering applications	183:218	bone tissue engineering applications	183:218	Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects.
25080396	6	2	theme	cells	1084:1088	arg1	activity					1044:1051	the osteogenic activity	1029:1051	the osteogenic activity of rabbit adipose-derived stem cells	1029:1088	In vitro osteogenic studies showed that the microspheres could enhance the osteogenic activity of rabbit adipose-derived stem cells.
25080396	1	3	theme	tissue	188:193	arg1	applications					207:218	bone tissue engineering applications	183:218	bone tissue engineering applications	183:218	Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects.
25080396	6	4	theme	stem	1079:1082	arg1	cells					1084:1088	rabbit adipose-derived stem cells	1056:1088	rabbit adipose-derived stem cells	1056:1088	In vitro osteogenic studies showed that the microspheres could enhance the osteogenic activity of rabbit adipose-derived stem cells.
25080396	6	5	theme	osteogenic	967:976	arg1	studies					978:984	In vitro osteogenic studies	958:984	In vitro osteogenic studies	958:984	In vitro osteogenic studies showed that the microspheres could enhance the osteogenic activity of rabbit adipose-derived stem cells.
25080396	6	6	theme	adipose-derived	1063:1077	arg1	cells					1084:1088	rabbit adipose-derived stem cells	1056:1088	rabbit adipose-derived stem cells	1056:1088	In vitro osteogenic studies showed that the microspheres could enhance the osteogenic activity of rabbit adipose-derived stem cells.
25080396	6	7	link	adipose-derived	1063:1077	arg1	cells					1084:1088	rabbit adipose-derived stem cells	1056:1088	rabbit adipose-derived stem cells	1056:1088	In vitro osteogenic studies showed that the microspheres could enhance the osteogenic activity of rabbit adipose-derived stem cells.
25080396	2	8	theme	particular	300:309	arg1	attention					311:319	particular attention	300:319	particular attention for drug delivery and bone tissue engineering	300:365	Chitosan (CH) has attracted particular attention for drug delivery and bone tissue engineering because of its favorable biocompatibility and biodegradability.
25080396	2	9	theme	bone	343:346	arg1	engineering					355:365	bone tissue engineering	343:365	bone tissue engineering	343:365	Chitosan (CH) has attracted particular attention for drug delivery and bone tissue engineering because of its favorable biocompatibility and biodegradability.
25080396	5	10	theme	loading	795:801	arg1	efficiency					803:812	loading efficiency	795:812	loading efficiency	795:812	The results showed that loading efficiency and sustained release of hydrophilic AL were significantly improved, which is ideal for locally sustained release in the bone microenvironment.
25080396	6	11	theme	In	958:959	arg1	studies					978:984	In vitro osteogenic studies	958:984	In vitro osteogenic studies	958:984	In vitro osteogenic studies showed that the microspheres could enhance the osteogenic activity of rabbit adipose-derived stem cells.
25080396	1	12	theme	Sustained	102:110	arg1	delivery					112:119	Sustained delivery	102:119	Sustained delivery of growth factors	102:137	Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects.
25080396	4	13	theme	drug	723:726	arg1	efficiency					736:745	drug loading efficiency	723:745	drug loading efficiency	723:745	The microspheres were characterized for their surface morphology, composition, size distribution, drug loading efficiency and release properties.
25080396	1	14	theme	engineering	195:205	arg1	applications					207:218	bone tissue engineering applications	183:218	bone tissue engineering applications	183:218	Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects.
25080396	6	15	theme	rabbit	1056:1061	arg1	cells					1084:1088	rabbit adipose-derived stem cells	1056:1088	rabbit adipose-derived stem cells	1056:1088	In vitro osteogenic studies showed that the microspheres could enhance the osteogenic activity of rabbit adipose-derived stem cells.
25080396	7	16	theme	local	1191:1195	arg1	treatment					1197:1205	local treatment	1191:1205	local treatment for bone defects	1191:1222	In conclusion, the CH/nHA-AL composite microspheres exhibit promising properties as a candidate for local treatment for bone defects.
25080396	4	17	theme	size	704:707	arg1	distribution					709:720	size distribution	704:720	size distribution	704:720	The microspheres were characterized for their surface morphology, composition, size distribution, drug loading efficiency and release properties.
25080396	3	18	theme	cross-linking	599:611	arg1	strategies					613:622	both emulsification and cross-linking strategies	575:622	both emulsification and cross-linking strategies	575:622	In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies.
25080396	3	19	theme	composite	448:456	arg1	system					470:475	a composite microsphere system	446:475	a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles	446:545	In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies.
25080396	1	20	theme	growth	124:129	arg1	factors					131:137	growth factors	124:137	growth factors	124:137	Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects.
25080396	3	21	theme	microsphere	458:468	arg1	system					470:475	a composite microsphere system	446:475	a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles	446:545	In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies.
25080396	7	22	theme	bone	1211:1214	arg1	defects					1216:1222	bone defects	1211:1222	bone defects	1211:1222	In conclusion, the CH/nHA-AL composite microspheres exhibit promising properties as a candidate for local treatment for bone defects.
25080396	2	23	theme	drug	325:328	arg1	delivery					330:337	drug delivery	325:337	drug delivery	325:337	Chitosan (CH) has attracted particular attention for drug delivery and bone tissue engineering because of its favorable biocompatibility and biodegradability.
25080396	3	24	theme	nanohydroxyapatite	495:512	arg1	particles					537:545	CH and nanohydroxyapatite (nHA)-alendronate (AL) particles	488:545	CH and nanohydroxyapatite (nHA)-alendronate (AL) particles	488:545	In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies.
25080396	0	25	theme	chitosan/nanohydroxyapatite	22:48	arg1	microspheres					50:61	chitosan/nanohydroxyapatite microspheres	22:61	chitosan/nanohydroxyapatite microspheres	22:61	Emulsion cross-linked chitosan/nanohydroxyapatite microspheres for controlled release of alendronate.
25080396	6	26	theme	osteogenic	1033:1042	arg1	activity					1044:1051	the osteogenic activity	1029:1051	the osteogenic activity of rabbit adipose-derived stem cells	1029:1088	In vitro osteogenic studies showed that the microspheres could enhance the osteogenic activity of rabbit adipose-derived stem cells.
25080396	0	27	theme	controlled	67:76	arg1	release					78:84	controlled release	67:84	controlled release of alendronate	67:99	Emulsion cross-linked chitosan/nanohydroxyapatite microspheres for controlled release of alendronate.
25080396	3	28	theme	CH	488:489	arg1	particles					537:545	CH and nanohydroxyapatite (nHA)-alendronate (AL) particles	488:545	CH and nanohydroxyapatite (nHA)-alendronate (AL) particles	488:545	In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies.
25080396	1	29	theme	factors	131:137	arg1	delivery					112:119	Sustained delivery	102:119	Sustained delivery of growth factors	102:137	Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects.
25080396	1	30	theme	various	241:247	arg1	kinds					249:253	various kinds	241:253	various kinds of bone defects	241:269	Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects.
25080396	5	31	theme	AL	851:852	arg1	efficiency					803:812	loading efficiency	795:812	loading efficiency	795:812	The results showed that loading efficiency and sustained release of hydrophilic AL were significantly improved, which is ideal for locally sustained release in the bone microenvironment.
25080396	5	31	theme	AL	851:852	arg1	release					828:834	sustained release	818:834	sustained release of hydrophilic AL	818:852	The results showed that loading efficiency and sustained release of hydrophilic AL were significantly improved, which is ideal for locally sustained release in the bone microenvironment.
25080396	7	32	theme	promising	1151:1159	arg1	properties					1161:1170	promising properties	1151:1170	promising properties	1151:1170	In conclusion, the CH/nHA-AL composite microspheres exhibit promising properties as a candidate for local treatment for bone defects.
25080396	7	32	theme	promising	1151:1159	arg1	candidate					1177:1185	a candidate	1175:1185	a candidate for local treatment for bone defects	1175:1222	In conclusion, the CH/nHA-AL composite microspheres exhibit promising properties as a candidate for local treatment for bone defects.
25080396	4	33	theme	surface	671:677	arg1	morphology					679:688	their surface morphology	665:688	their surface morphology	665:688	The microspheres were characterized for their surface morphology, composition, size distribution, drug loading efficiency and release properties.
25080396	1	34	theme	kinds	249:253	arg1	treatment					228:236	the treatment	224:236	the treatment of various kinds of bone defects	224:269	Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects.
25080396	7	35	theme	composite	1120:1128	arg1	microspheres					1130:1141	the CH/nHA-AL composite microspheres	1106:1141	the CH/nHA-AL composite microspheres	1106:1141	In conclusion, the CH/nHA-AL composite microspheres exhibit promising properties as a candidate for local treatment for bone defects.
25080396	5	36	theme	sustained	818:826	arg1	release					828:834	sustained release	818:834	sustained release of hydrophilic AL	818:852	The results showed that loading efficiency and sustained release of hydrophilic AL were significantly improved, which is ideal for locally sustained release in the bone microenvironment.
25080396	3	37	theme	-alendronate	519:530	arg1	nHA					515:517	nHA	515:517	nHA	515:517	In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies.
25080396	3	37	theme	-alendronate	519:530	arg1	nanohydroxyapatite					495:512	nanohydroxyapatite (nHA)-alendronate (AL)	495:535	nanohydroxyapatite (nHA)-alendronate (AL)	495:535	In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies.
25080396	5	38	theme	bone	935:938	arg1	microenvironment					940:955	the bone microenvironment	931:955	the bone microenvironment	931:955	The results showed that loading efficiency and sustained release of hydrophilic AL were significantly improved, which is ideal for locally sustained release in the bone microenvironment.
25080396	4	39	theme	loading	728:734	arg1	efficiency					736:745	drug loading efficiency	723:745	drug loading efficiency	723:745	The microspheres were characterized for their surface morphology, composition, size distribution, drug loading efficiency and release properties.
25080396	2	40	theme	tissue	348:353	arg1	engineering					355:365	bone tissue engineering	343:365	bone tissue engineering	343:365	Chitosan (CH) has attracted particular attention for drug delivery and bone tissue engineering because of its favorable biocompatibility and biodegradability.
25080396	3	41	theme	emulsification	580:593	arg1	strategies					613:622	both emulsification and cross-linking strategies	575:622	both emulsification and cross-linking strategies	575:622	In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies.
25080396	7	42	theme	CH/nHA-AL	1110:1118	arg1	microspheres					1130:1141	the CH/nHA-AL composite microspheres	1106:1141	the CH/nHA-AL composite microspheres	1106:1141	In conclusion, the CH/nHA-AL composite microspheres exhibit promising properties as a candidate for local treatment for bone defects.
25080396	3	43	contain	containing	477:486	arg1	system					470:475	a composite microsphere system	446:475	a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles	446:545	In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies.
25080396	3	43	contain	containing	477:486	arg2	particles					537:545	CH and nanohydroxyapatite (nHA)-alendronate (AL) particles	488:545	CH and nanohydroxyapatite (nHA)-alendronate (AL) particles	488:545	In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies.
25080396	1	44	theme	essential	157:165	arg1	requirement					167:177	an essential requirement	154:177	an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects	154:269	Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects.
25080396	1	45	theme	bone	258:261	arg1	defects					263:269	bone defects	258:269	bone defects	258:269	Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects.
25080396	5	46	theme	sustained	910:918	arg1	release					920:926	locally sustained release	902:926	locally sustained release in the bone microenvironment	902:955	The results showed that loading efficiency and sustained release of hydrophilic AL were significantly improved, which is ideal for locally sustained release in the bone microenvironment.
25080396	3	47	dep	nanohydroxyapatite	495:512	arg1	AL					533:534	AL	533:534	AL	533:534	In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies.
25080396	1	48	theme	defects	263:269	arg1	kinds					249:253	various kinds	241:253	various kinds of bone defects	241:269	Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects.
25080396	0	49	theme	alendronate	89:99	arg1	release					78:84	controlled release	67:84	controlled release of alendronate	67:99	Emulsion cross-linked chitosan/nanohydroxyapatite microspheres for controlled release of alendronate.
25080396	5	50	from	release	920:926	arg1	microenvironment					940:955	the bone microenvironment	931:955	the bone microenvironment	931:955	The results showed that loading efficiency and sustained release of hydrophilic AL were significantly improved, which is ideal for locally sustained release in the bone microenvironment.
25080396	2	51	theme	favorable	382:390	arg1	biocompatibility					392:407	its favorable biocompatibility	378:407	its favorable biocompatibility	378:407	Chitosan (CH) has attracted particular attention for drug delivery and bone tissue engineering because of its favorable biocompatibility and biodegradability.
25080396	6	52	dep	In	958:959	arg1	vitro					961:965	vitro	961:965	vitro	961:965	In vitro osteogenic studies showed that the microspheres could enhance the osteogenic activity of rabbit adipose-derived stem cells.
26233725	0	0	theme	controlled	95:104	arg1	release					106:112	the controlled release	91:112	the controlled release of silk sericin	91:128	The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin.
26233725	7	1	theme	release	1113:1119	arg1	sericin					1121:1127	release sericin	1113:1127	release sericin in the most sustained behavior	1113:1158	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	12	2	theme	CH/SS	1795:1799	arg1	microspheres					1801:1812	the non-toxic ionic-crosslinked CH/SS microspheres	1763:1812	the non-toxic ionic-crosslinked CH/SS microspheres	1763:1812	Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing.
26233725	7	3	theme	charged	1224:1230	arg1	chitosan					1232:1239	the positively charged chitosan	1209:1239	the positively charged chitosan	1209:1239	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	12	4	theme	dressing	1845:1852	arg1	material					1854:1861	wound dressing material	1839:1861	wound dressing material	1839:1861	Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing.
26233725	5	5	theme	narrow	775:780	arg1	distribution					787:798	narrow size distribution	775:798	narrow size distribution	775:798	The microspheres with an average size of 1-4 μm and narrow size distribution were obtained.
26233725	12	6	theme	wound	1923:1927	arg1	healing					1929:1935	accelerated wound healing	1911:1935	accelerated wound healing	1911:1935	Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing.
26233725	4	7	theme	polyanionic	698:708	arg1	crosslinker					710:720	a polyanionic crosslinker	696:720	a polyanionic crosslinker	696:720	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustri-polyphosphate (TPP) as a polyanionic crosslinker.
26233725	4	7	theme	polyanionic	698:708	arg1	anhydroustri-polyphosphate					660:685	anhydroustri-polyphosphate	660:685	anhydroustri-polyphosphate (TPP)	660:691	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustri-polyphosphate (TPP) as a polyanionic crosslinker.
26233725	9	8	theme	enzymatic	1456:1464	arg1	degradation					1466:1476	enzymatic degradation	1456:1476	enzymatic degradation	1456:1476	All microspheres continuously degraded and remained around 20% after 14 days of enzymatic degradation.
26233725	7	9	theme	strong	1181:1186	arg1	interaction					1194:1204	the strong ionic interaction	1177:1204	the strong ionic interaction of the positively charged chitosan and the negatively charged sericin	1177:1274	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	4	10	theme	chitosan/sericin	529:544	arg1	microspheres					554:565	The chitosan/sericin (CH/SS) microspheres	525:565	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50)	525:623	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustri-polyphosphate (TPP) as a polyanionic crosslinker.
26233725	6	11	theme	FT-IR	820:824	arg1	spectra					826:832	FT-IR spectra	820:832	FT-IR spectra	820:832	From FT-IR spectra, the presence of both chitosan and sericin in the microspheres confirmed the occurrence of ionic interaction that crosslink them within the microspheres.
26233725	2	12	theme	few	309:311	arg1	researches					313:322	very few researches	304:322	very few researches	304:322	While various sericin carriers were developed for drug delivery system, very few researches considered sericin as a bioactive molecule itself.
26233725	8	13	theme	degradation	1342:1352	arg1	rate					1354:1357	the degradation rate	1338:1357	the degradation rate of microspheres	1338:1373	On the other hand, the composition of CH/SS had no effect on the degradation rate of microspheres.
26233725	5	14	theme	average	748:754	arg1	size					756:759	an average size	745:759	an average size of 1-4 μm	745:769	The microspheres with an average size of 1-4 μm and narrow size distribution were obtained.
26233725	1	15	contain	possess	165:171	arg1	sericin					136:142	Silk sericin	131:142	Silk sericin	131:142	Silk sericin is recently shown to possess various biological activities for biomedical applications.
26233725	1	15	contain	possess	165:171	arg2	activities					192:201	various biological activities	173:201	various biological activities for biomedical applications	173:229	Silk sericin is recently shown to possess various biological activities for biomedical applications.
26233725	8	16	theme	CH/SS	1315:1319	arg1	composition					1300:1310	the composition	1296:1310	the composition of CH/SS	1296:1319	On the other hand, the composition of CH/SS had no effect on the degradation rate of microspheres.
26233725	12	17	theme	sustained	1878:1886	arg1	release					1888:1894	the sustained release	1874:1894	the sustained release of sericin for accelerated wound healing	1874:1935	Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing.
26233725	8	18	theme	other	1284:1288	arg1	hand					1290:1293	the other hand	1280:1293	the other hand	1280:1293	On the other hand, the composition of CH/SS had no effect on the degradation rate of microspheres.
26233725	0	19	theme	sericin	122:128	arg1	release					106:112	the controlled release	91:112	the controlled release of silk sericin	91:128	The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin.
26233725	6	20	attach	presence	839:846	arg1	microspheres					884:895	the microspheres	880:895	the microspheres	880:895	From FT-IR spectra, the presence of both chitosan and sericin in the microspheres confirmed the occurrence of ionic interaction that crosslink them within the microspheres.
26233725	6	20	attach	presence	839:846	arg2	sericin					869:875	sericin	869:875	sericin	869:875	From FT-IR spectra, the presence of both chitosan and sericin in the microspheres confirmed the occurrence of ionic interaction that crosslink them within the microspheres.
26233725	6	20	attach	presence	839:846	arg2	chitosan					856:863	chitosan	856:863	chitosan	856:863	From FT-IR spectra, the presence of both chitosan and sericin in the microspheres confirmed the occurrence of ionic interaction that crosslink them within the microspheres.
26233725	12	21	theme	sericin	1899:1905	arg1	release					1888:1894	the sustained release	1874:1894	the sustained release of sericin for accelerated wound healing	1874:1935	Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing.
26233725	1	22	theme	various	173:179	arg1	activities					192:201	various biological activities	173:201	various biological activities for biomedical applications	173:229	Silk sericin is recently shown to possess various biological activities for biomedical applications.
26233725	0	23	theme	silk	117:120	arg1	sericin					122:128	silk sericin	117:128	silk sericin	117:128	The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin.
26233725	4	24	dep	composition	580:590	arg1	60/40					607:611	60/40	607:611	60/40	607:611	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustri-polyphosphate (TPP) as a polyanionic crosslinker.
26233725	4	24	dep	composition	580:590	arg1	50/50					618:622	50/50	618:622	50/50	618:622	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustri-polyphosphate (TPP) as a polyanionic crosslinker.
26233725	4	24	dep	composition	580:590	arg1	70/30					600:604	70/30	600:604	70/30	600:604	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustri-polyphosphate (TPP) as a polyanionic crosslinker.
26233725	4	24	dep	composition	580:590	arg1	80/20					593:597	80/20	593:597	80/20	593:597	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustri-polyphosphate (TPP) as a polyanionic crosslinker.
26233725	5	25	theme	size	782:785	arg1	distribution					787:798	narrow size distribution	775:798	narrow size distribution	775:798	The microspheres with an average size of 1-4 μm and narrow size distribution were obtained.
26233725	9	26	theme	degradation	1466:1476	arg1	days					1448:1451	14 days	1445:1451	14 days of enzymatic degradation	1445:1476	All microspheres continuously degraded and remained around 20% after 14 days of enzymatic degradation.
26233725	1	27	theme	biological	181:190	arg1	activities					192:201	various biological activities	173:201	various biological activities for biomedical applications	173:229	Silk sericin is recently shown to possess various biological activities for biomedical applications.
26233725	11	28	theme	CH/SS	1641:1645	arg1	microspheres					1647:1658	all CH/SS microspheres	1637:1658	all CH/SS microspheres at any concentrations	1637:1680	Furthermore, we have verified that all CH/SS microspheres at any concentrations showed non-toxicity to L929 mouse fibroblast cells.
26233725	12	29	theme	accelerated	1911:1921	arg1	healing					1929:1935	accelerated wound healing	1911:1935	accelerated wound healing	1911:1935	Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing.
26233725	3	30	theme	chitosan-based	418:431	arg1	microspheres					433:444	the chitosan-based microspheres	414:444	the chitosan-based microspheres	414:444	In this study, sericin incorporated in the chitosan-based microspheres was introduced as a bioactive molecule and bioactive carrier at the same time.
26233725	12	31	theme	wound	1839:1843	arg1	material					1854:1861	wound dressing material	1839:1861	wound dressing material	1839:1861	Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing.
26233725	7	32	from	percentage	1089:1098	arg1	behavior					1151:1158	the most sustained behavior	1132:1158	the most sustained behavior	1132:1158	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	10	33	dep	enzyme	1579:1584	arg1	the					1575:1577	the	1575:1577	the	1575:1577	This explained that the ionic crosslinkings between chitosan and sericin could be demolished by the enzyme and hydrolysis.
26233725	0	34	theme	non-toxic	19:27	arg1	microspheres					62:73	non-toxic ionic-crosslinked chitosan-based microspheres	19:73	non-toxic ionic-crosslinked chitosan-based microspheres	19:73	The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin.
26233725	4	35	theme	different	570:578	arg1	composition					580:590	different composition	570:590	different composition (80/20, 70/30, 60/40, and 50/50)	570:623	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustri-polyphosphate (TPP) as a polyanionic crosslinker.
26233725	0	36	link	ionic-crosslinked	29:45	arg1	microspheres					62:73	non-toxic ionic-crosslinked chitosan-based microspheres	19:73	non-toxic ionic-crosslinked chitosan-based microspheres	19:73	The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin.
26233725	3	37	theme	bioactive	489:497	arg1	sericin					390:396	sericin	390:396	sericin incorporated in the chitosan-based microspheres	390:444	In this study, sericin incorporated in the chitosan-based microspheres was introduced as a bioactive molecule and bioactive carrier at the same time.
26233725	3	37	theme	bioactive	489:497	arg1	carrier					499:505	bioactive carrier	489:505	bioactive carrier	489:505	In this study, sericin incorporated in the chitosan-based microspheres was introduced as a bioactive molecule and bioactive carrier at the same time.
26233725	12	38	theme	non-toxic	1767:1775	arg1	microspheres					1801:1812	the non-toxic ionic-crosslinked CH/SS microspheres	1763:1812	the non-toxic ionic-crosslinked CH/SS microspheres	1763:1812	Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing.
26233725	7	39	theme	highest	1081:1087	arg1	percentage					1089:1098	the highest percentage	1077:1098	the highest percentage (37.28%)	1077:1107	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	7	39	theme	highest	1081:1087	arg1	%					1106:1106	37.28%	1101:1106	37.28%	1101:1106	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	6	40	theme	sericin	869:875	arg1	presence					839:846	the presence	835:846	the presence of both chitosan and sericin in the microspheres	835:895	From FT-IR spectra, the presence of both chitosan and sericin in the microspheres confirmed the occurrence of ionic interaction that crosslink them within the microspheres.
26233725	7	41	theme	chitosan	1232:1239	arg1	interaction					1194:1204	the strong ionic interaction	1177:1204	the strong ionic interaction of the positively charged chitosan and the negatively charged sericin	1177:1274	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	0	42	theme	chitosan-based	47:60	arg1	microspheres					62:73	non-toxic ionic-crosslinked chitosan-based microspheres	19:73	non-toxic ionic-crosslinked chitosan-based microspheres	19:73	The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin.
26233725	5	43	with	microspheres	727:738	arg1	size					756:759	an average size	745:759	an average size of 1-4 μm	745:769	The microspheres with an average size of 1-4 μm and narrow size distribution were obtained.
26233725	5	43	with	microspheres	727:738	arg1	distribution					787:798	narrow size distribution	775:798	narrow size distribution	775:798	The microspheres with an average size of 1-4 μm and narrow size distribution were obtained.
26233725	8	44	theme	microspheres	1362:1373	arg1	rate					1354:1357	the degradation rate	1338:1357	the degradation rate of microspheres	1338:1373	On the other hand, the composition of CH/SS had no effect on the degradation rate of microspheres.
26233725	0	45	theme	ionic-crosslinked	29:45	arg1	microspheres					62:73	non-toxic ionic-crosslinked chitosan-based microspheres	19:73	non-toxic ionic-crosslinked chitosan-based microspheres	19:73	The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin.
26233725	7	46	theme	sustained	1141:1149	arg1	behavior					1151:1158	the most sustained behavior	1132:1158	the most sustained behavior	1132:1158	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	6	47	theme	chitosan	856:863	arg1	presence					839:846	the presence	835:846	the presence of both chitosan and sericin in the microspheres	835:895	From FT-IR spectra, the presence of both chitosan and sericin in the microspheres confirmed the occurrence of ionic interaction that crosslink them within the microspheres.
26233725	4	48	from	composition	580:590	arg1	microspheres					554:565	The chitosan/sericin (CH/SS) microspheres	525:565	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50)	525:623	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustri-polyphosphate (TPP) as a polyanionic crosslinker.
26233725	6	49	from	presence	839:846	arg1	microspheres					884:895	the microspheres	880:895	the microspheres	880:895	From FT-IR spectra, the presence of both chitosan and sericin in the microspheres confirmed the occurrence of ionic interaction that crosslink them within the microspheres.
26233725	7	50	from	sericin	1121:1127	arg1	behavior					1151:1158	the most sustained behavior	1132:1158	the most sustained behavior	1132:1158	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	12	51	link	ionic-crosslinked	1777:1793	arg1	microspheres					1801:1812	the non-toxic ionic-crosslinked CH/SS microspheres	1763:1812	the non-toxic ionic-crosslinked CH/SS microspheres	1763:1812	Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing.
26233725	4	52	theme	CH/SS	547:551	arg1	microspheres					554:565	The chitosan/sericin (CH/SS) microspheres	525:565	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50)	525:623	The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustri-polyphosphate (TPP) as a polyanionic crosslinker.
26233725	10	53	theme	ionic	1503:1507	arg1	crosslinkings					1509:1521	the ionic crosslinkings	1499:1521	the ionic crosslinkings between chitosan and sericin	1499:1550	This explained that the ionic crosslinkings between chitosan and sericin could be demolished by the enzyme and hydrolysis.
26233725	2	54	theme	sericin	246:252	arg1	carriers					254:261	various sericin carriers	238:261	various sericin carriers	238:261	While various sericin carriers were developed for drug delivery system, very few researches considered sericin as a bioactive molecule itself.
26233725	3	55	theme	same	514:517	arg1	time					519:522	the same time	510:522	the same time	510:522	In this study, sericin incorporated in the chitosan-based microspheres was introduced as a bioactive molecule and bioactive carrier at the same time.
26233725	0	56	theme	microspheres	62:73	arg1	development					4:14	The development	0:14	The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin.	0:129	The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin.
26233725	8	57	contain	had	1321:1323	arg2	effect					1328:1333	no effect	1325:1333	no effect	1325:1333	On the other hand, the composition of CH/SS had no effect on the degradation rate of microspheres.
26233725	8	57	contain	had	1321:1323	arg1	composition					1300:1310	the composition	1296:1310	the composition of CH/SS	1296:1319	On the other hand, the composition of CH/SS had no effect on the degradation rate of microspheres.
26233725	11	58	theme	mouse	1710:1714	arg1	cells					1727:1731	L929 mouse fibroblast cells	1705:1731	L929 mouse fibroblast cells	1705:1731	Furthermore, we have verified that all CH/SS microspheres at any concentrations showed non-toxicity to L929 mouse fibroblast cells.
26233725	2	59	theme	various	238:244	arg1	carriers					254:261	various sericin carriers	238:261	various sericin carriers	238:261	While various sericin carriers were developed for drug delivery system, very few researches considered sericin as a bioactive molecule itself.
26233725	7	60	theme	sericin	1268:1274	arg1	interaction					1194:1204	the strong ionic interaction	1177:1204	the strong ionic interaction of the positively charged chitosan and the negatively charged sericin	1177:1274	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	1	61	theme	Silk	131:134	arg1	sericin					136:142	Silk sericin	131:142	Silk sericin	131:142	Silk sericin is recently shown to possess various biological activities for biomedical applications.
26233725	6	62	theme	interaction	931:941	arg1	occurrence					911:920	the occurrence	907:920	the occurrence of ionic interaction that crosslink them within the microspheres	907:985	From FT-IR spectra, the presence of both chitosan and sericin in the microspheres confirmed the occurrence of ionic interaction that crosslink them within the microspheres.
26233725	11	63	theme	fibroblast	1716:1725	arg1	cells					1727:1731	L929 mouse fibroblast cells	1705:1731	L929 mouse fibroblast cells	1705:1731	Furthermore, we have verified that all CH/SS microspheres at any concentrations showed non-toxicity to L929 mouse fibroblast cells.
26233725	2	64	theme	delivery	287:294	arg1	system					296:301	drug delivery system	282:301	drug delivery system	282:301	While various sericin carriers were developed for drug delivery system, very few researches considered sericin as a bioactive molecule itself.
26233725	7	65	theme	CH/SS	1011:1015	arg1	microspheres					1017:1028	the CH/SS microspheres	1007:1028	the CH/SS microspheres prepared at 50/50	1007:1046	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	2	66	theme	bioactive	348:356	arg1	molecule					358:365	a bioactive molecule	346:365	a bioactive molecule itself	346:372	While various sericin carriers were developed for drug delivery system, very few researches considered sericin as a bioactive molecule itself.
26233725	2	66	theme	bioactive	348:356	arg1	sericin					335:341	sericin	335:341	sericin	335:341	While various sericin carriers were developed for drug delivery system, very few researches considered sericin as a bioactive molecule itself.
26233725	2	67	theme	drug	282:285	arg1	system					296:301	drug delivery system	282:301	drug delivery system	282:301	While various sericin carriers were developed for drug delivery system, very few researches considered sericin as a bioactive molecule itself.
26233725	12	68	theme	ionic-crosslinked	1777:1793	arg1	microspheres					1801:1812	the non-toxic ionic-crosslinked CH/SS microspheres	1763:1812	the non-toxic ionic-crosslinked CH/SS microspheres	1763:1812	Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing.
26233725	7	69	theme	charged	1260:1266	arg1	sericin					1268:1274	the negatively charged sericin	1245:1274	the negatively charged sericin	1245:1274	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
26233725	11	70	theme	L929	1705:1708	arg1	cells					1727:1731	L929 mouse fibroblast cells	1705:1731	L929 mouse fibroblast cells	1705:1731	Furthermore, we have verified that all CH/SS microspheres at any concentrations showed non-toxicity to L929 mouse fibroblast cells.
26233725	1	71	theme	biomedical	207:216	arg1	applications					218:229	biomedical applications	207:229	biomedical applications	207:229	Silk sericin is recently shown to possess various biological activities for biomedical applications.
26233725	3	72	theme	bioactive	466:474	arg1	sericin					390:396	sericin	390:396	sericin incorporated in the chitosan-based microspheres	390:444	In this study, sericin incorporated in the chitosan-based microspheres was introduced as a bioactive molecule and bioactive carrier at the same time.
26233725	3	72	theme	bioactive	466:474	arg1	molecule					476:483	a bioactive molecule	464:483	a bioactive molecule	464:483	In this study, sericin incorporated in the chitosan-based microspheres was introduced as a bioactive molecule and bioactive carrier at the same time.
26233725	11	73	from	concentrations	1667:1680	arg1	microspheres					1647:1658	all CH/SS microspheres	1637:1658	all CH/SS microspheres at any concentrations	1637:1680	Furthermore, we have verified that all CH/SS microspheres at any concentrations showed non-toxicity to L929 mouse fibroblast cells.
26233725	5	74	theme	μm	768:769	arg1	size					756:759	an average size	745:759	an average size of 1-4 μm	745:769	The microspheres with an average size of 1-4 μm and narrow size distribution were obtained.
26233725	5	74	theme	μm	768:769	arg1	distribution					787:798	narrow size distribution	775:798	narrow size distribution	775:798	The microspheres with an average size of 1-4 μm and narrow size distribution were obtained.
26233725	6	75	theme	ionic	925:929	arg1	interaction					931:941	ionic interaction	925:941	ionic interaction that crosslink them within the microspheres	925:985	From FT-IR spectra, the presence of both chitosan and sericin in the microspheres confirmed the occurrence of ionic interaction that crosslink them within the microspheres.
26233725	7	76	theme	ionic	1188:1192	arg1	interaction					1194:1204	the strong ionic interaction	1177:1204	the strong ionic interaction of the positively charged chitosan and the negatively charged sericin	1177:1274	We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin.
27164875	2	0	theme	treated	492:498	arg1	solutions					500:508	treated solutions	492:508	treated solutions	492:508	Nonetheless, since they consist of microparticles, their practical use as an adsorbent is limited by difficulties with separation from treated solutions.
27164875	5	1	theme	adsorption	850:859	arg1	process					861:867	the adsorption process	846:867	the adsorption process	846:867	In order to determine the key parameters of the adsorption process, both equilibrium and kinetic studies were conducted.
27164875	3	2	theme	sorbent	672:678	arg1	properties					635:644	sorptive properties	626:644	sorptive properties of the obtained composite sorbent toward As(III) and As(V)	626:703	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	9	3	theme	groups	1332:1337	arg1	presence					1309:1316	The presence	1305:1316	The presence of carboxylic groups in polymer chains	1305:1355	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	1	4	theme	large	162:166	arg1	quantities					168:177	large quantities	162:177	large quantities	162:177	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	5	5	theme	kinetic	891:897	arg1	studies					899:905	kinetic studies	891:905	kinetic studies	891:905	In order to determine the key parameters of the adsorption process, both equilibrium and kinetic studies were conducted.
27164875	9	6	theme	arsenates	1511:1519	arg1	case					1503:1506	the case	1499:1506	the case of arsenates	1499:1519	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	4	7	theme	matrix	794:799	arg1	density					774:780	density	774:780	density of alginate matrix	774:799	Different products were formed varying in WTR content as well as in density of alginate matrix.
27164875	9	8	theme	sorbent	1416:1422	arg1	beads					1424:1428	sorbent beads	1416:1428	sorbent beads	1416:1428	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	3	9	theme	pretreated	562:571	arg1	WTR					573:575	chemically pretreated WTR	551:575	chemically pretreated WTR	551:575	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	1	10	theme	iron	262:265	arg1	content					251:257	high content	246:257	high content of iron and manganese oxides	246:286	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	0	11	theme	adsorption	97:106	arg1	studies					108:114	adsorption studies	97:114	adsorption studies	97:114	Alginate beads containing water treatment residuals for arsenic removal from water-formation and adsorption studies.
27164875	9	12	theme	rate-limiting	1451:1463	arg1	step					1465:1468	the main rate-limiting step	1442:1468	the main rate-limiting step	1442:1468	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	9	12	theme	rate-limiting	1451:1463	arg1	diffusion					1540:1548	intraparticle diffusion	1526:1548	intraparticle diffusion	1526:1548	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	8	13	theme	adsorption	1203:1212	arg1	effectiveness					1214:1226	the adsorption effectiveness	1199:1226	the adsorption effectiveness	1199:1226	At neutral pH, the adsorption effectiveness decreased to 3.3 mg As g-1 for arsenites and to 0.7 mg As g-1 for arsenates.
27164875	6	14	contain	containing	971:980	arg2	residuals					986:994	5 % residuals	982:994	5 % residuals	982:994	The best properties were exhibited by a sorbent containing 5 % residuals, formed in alginate solution with a concentration of 1 %.
27164875	6	14	contain	containing	971:980	arg1	sorbent					963:969	a sorbent	961:969	a sorbent containing 5 % residuals, formed in alginate solution with a concentration of 1 %	961:1051	The best properties were exhibited by a sorbent containing 5 % residuals, formed in alginate solution with a concentration of 1 %.
27164875	6	15	with	solution	1016:1023	arg1	concentration					1032:1044	a concentration	1030:1044	a concentration of 1 %	1030:1051	The best properties were exhibited by a sorbent containing 5 % residuals, formed in alginate solution with a concentration of 1 %.
27164875	8	16	theme	0.7 mg	1276:1281	arg1	As g-1					1283:1288	0.7 mg As g-1	1276:1288	0.7 mg As g-1 for arsenates	1276:1302	At neutral pH, the adsorption effectiveness decreased to 3.3 mg As g-1 for arsenites and to 0.7 mg As g-1 for arsenates.
27164875	3	17	theme	study	527:531	arg1	entrapment					537:546	entrapment	537:546	entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V)	537:703	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	3	17	theme	study	527:531	arg1	aim					515:517	The aim	511:517	The aim of this study	511:531	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	9	18	theme	carboxylic	1321:1330	arg1	groups					1332:1337	carboxylic groups	1321:1337	carboxylic groups	1321:1337	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	5	19	theme	process	861:867	arg1	parameters					832:841	the key parameters	824:841	the key parameters of the adsorption process	824:867	In order to determine the key parameters of the adsorption process, both equilibrium and kinetic studies were conducted.
27164875	9	20	theme	intraparticle	1526:1538	arg1	step					1465:1468	the main rate-limiting step	1442:1468	the main rate-limiting step	1442:1468	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	9	20	theme	intraparticle	1526:1538	arg1	diffusion					1540:1548	intraparticle diffusion	1526:1548	intraparticle diffusion	1526:1548	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	9	21	from	conditions	1376:1385	arg1	impeded					1357:1363	impeded	1357:1363	impeded	1357:1363	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	4	22	theme	WTR	748:750	arg1	content					752:758	WTR content	748:758	WTR content	748:758	Different products were formed varying in WTR content as well as in density of alginate matrix.
27164875	7	23	theme	sorption	1106:1113	arg1	capacity					1115:1122	its maximum sorption capacity	1094:1122	its maximum sorption capacity	1094:1122	In slightly acidic conditions (pH 4.5), its maximum sorption capacity was 3.4 and 2.9 mg g-1 for As(III) and As(V), respectively.
27164875	3	24	theme	composite	662:670	arg1	sorbent					672:678	the obtained composite sorbent	649:678	the obtained composite sorbent toward As(III) and As(V)	649:703	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	10	25	theme	arsenites	1615:1623	arg1	removal					1590:1596	simultaneous removal	1577:1596	simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent	1577:1677	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	9	26	theme	anions	1404:1409	arg1	diffusion					1391:1399	the diffusion	1387:1399	the diffusion of anions into sorbent beads	1387:1428	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	3	27	theme	WTR	573:575	arg1	entrapment					537:546	entrapment	537:546	entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V)	537:703	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	3	27	theme	WTR	573:575	arg1	aim					515:517	The aim	511:517	The aim of this study	511:531	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	9	28	theme	adsorption	1477:1486	arg1	step					1465:1468	the main rate-limiting step	1442:1468	the main rate-limiting step	1442:1468	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	9	28	theme	adsorption	1477:1486	arg1	diffusion					1540:1548	intraparticle diffusion	1526:1548	intraparticle diffusion	1526:1548	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	5	29	theme	key	828:830	arg1	parameters					832:841	the key parameters	824:841	the key parameters of the adsorption process	824:867	In order to determine the key parameters of the adsorption process, both equilibrium and kinetic studies were conducted.
27164875	0	30	theme	Alginate	0:7	arg1	beads					9:13	Alginate beads	0:13	Alginate beads	0:13	Alginate beads containing water treatment residuals for arsenic removal from water-formation and adsorption studies.
27164875	1	31	theme	manganese	271:279	arg1	content					251:257	high content	246:257	high content of iron and manganese oxides	246:286	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	4	32	theme	Different	706:714	arg1	products					716:723	Different products	706:723	Different products	706:723	Different products were formed varying in WTR content as well as in density of alginate matrix.
27164875	0	33	from	water-formation	77:91	arg1	removal					64:70	arsenic removal	56:70	arsenic removal from water-formation and adsorption studies	56:114	Alginate beads containing water treatment residuals for arsenic removal from water-formation and adsorption studies.
27164875	0	34	theme	water	26:30	arg1	residuals					42:50	water treatment residuals	26:50	water treatment residuals	26:50	Alginate beads containing water treatment residuals for arsenic removal from water-formation and adsorption studies.
27164875	6	35	theme	best	927:930	arg1	properties					932:941	The best properties	923:941	The best properties	923:941	The best properties were exhibited by a sorbent containing 5 % residuals, formed in alginate solution with a concentration of 1 %.
27164875	4	36	theme	alginate	785:792	arg1	matrix					794:799	alginate matrix	785:799	alginate matrix	785:799	Different products were formed varying in WTR content as well as in density of alginate matrix.
27164875	9	37	theme	main	1446:1449	arg1	step					1465:1468	the main rate-limiting step	1442:1468	the main rate-limiting step	1442:1468	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	9	37	theme	main	1446:1449	arg1	diffusion					1540:1548	intraparticle diffusion	1526:1548	intraparticle diffusion	1526:1548	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	8	38	theme	3.3 mg	1241:1246	arg1	As g-1					1248:1253	3.3 mg As g-1	1241:1253	3.3 mg As g-1 for arsenites	1241:1267	At neutral pH, the adsorption effectiveness decreased to 3.3 mg As g-1 for arsenites and to 0.7 mg As g-1 for arsenates.
27164875	10	39	theme	composite	1661:1669	arg1	sorbent					1671:1677	the obtained composite sorbent	1648:1677	the obtained composite sorbent	1648:1677	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	3	40	theme	obtained	653:660	arg1	sorbent					672:678	the obtained composite sorbent	649:678	the obtained composite sorbent toward As(III) and As(V)	649:703	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	1	41	theme	Water	117:121	arg1	WTRs					144:147	WTRs	144:147	WTRs	144:147	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	1	41	theme	Water	117:121	arg1	residuals					133:141	Water treatment residuals	117:141	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides,	117:287	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	9	42	from	step	1465:1468	arg1	case					1503:1506	the case	1499:1506	the case of arsenates	1499:1519	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	9	43	theme	polymer	1342:1348	arg1	chains					1350:1355	polymer chains	1342:1355	polymer chains	1342:1355	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	9	44	dep	diffusion	1540:1548	arg1	presence					1309:1316	The presence	1305:1316	The presence of carboxylic groups in polymer chains	1305:1355	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	9	44	dep	diffusion	1540:1548	arg1	diffusion					1391:1399	the diffusion	1387:1399	the diffusion of anions into sorbent beads	1387:1428	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	9	44	dep	diffusion	1540:1548	arg1	impeded					1357:1363	impeded	1357:1363	impeded	1357:1363	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	9	45	theme	neutral	1368:1374	arg1	conditions					1376:1385	neutral conditions	1368:1385	neutral conditions	1368:1385	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	10	46	theme	obtained	1652:1659	arg1	sorbent					1671:1677	the obtained composite sorbent	1648:1677	the obtained composite sorbent	1648:1677	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	1	47	theme	infiltration	219:230	arg1	water					232:236	infiltration water	219:236	infiltration water	219:236	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	7	48	from	3.4	1128:1130	arg1	pH					1085:1086	pH 4.5	1085:1090	pH 4.5	1085:1090	In slightly acidic conditions (pH 4.5), its maximum sorption capacity was 3.4 and 2.9 mg g-1 for As(III) and As(V), respectively.
27164875	7	48	from	3.4	1128:1130	arg1	conditions					1073:1082	slightly acidic conditions	1057:1082	slightly acidic conditions (pH 4.5)	1057:1091	In slightly acidic conditions (pH 4.5), its maximum sorption capacity was 3.4 and 2.9 mg g-1 for As(III) and As(V), respectively.
27164875	6	49	theme	alginate	1007:1014	arg1	solution					1016:1023	alginate solution	1007:1023	alginate solution with a concentration of 1 %	1007:1051	The best properties were exhibited by a sorbent containing 5 % residuals, formed in alginate solution with a concentration of 1 %.
27164875	2	50	theme	practical	414:422	arg1	use					424:426	their practical use	408:426	their practical use as an adsorbent	408:442	Nonetheless, since they consist of microparticles, their practical use as an adsorbent is limited by difficulties with separation from treated solutions.
27164875	0	51	theme	treatment	32:40	arg1	residuals					42:50	water treatment residuals	26:50	water treatment residuals	26:50	Alginate beads containing water treatment residuals for arsenic removal from water-formation and adsorption studies.
27164875	1	52	theme	excellent	297:305	arg1	properties					316:325	excellent sorptive properties	297:325	excellent sorptive properties	297:325	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	10	53	theme	similar	1711:1717	arg1	effectiveness					1730:1742	similar adsorption effectiveness	1711:1742	similar adsorption effectiveness for both arsenic species	1711:1767	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	1	54	theme	water	232:236	arg1	deironing					186:194	deironing	186:194	deironing	186:194	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	1	54	theme	water	232:236	arg1	demanganization					200:214	demanganization	200:214	demanganization	200:214	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	0	55	theme	arsenic	56:62	arg1	removal					64:70	arsenic removal	56:70	arsenic removal from water-formation and adsorption studies	56:114	Alginate beads containing water treatment residuals for arsenic removal from water-formation and adsorption studies.
27164875	1	56	theme	sorptive	307:314	arg1	properties					316:325	excellent sorptive properties	297:325	excellent sorptive properties	297:325	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	2	57	from	solutions	500:508	arg1	separation					476:485	separation	476:485	separation from treated solutions	476:508	Nonetheless, since they consist of microparticles, their practical use as an adsorbent is limited by difficulties with separation from treated solutions.
27164875	3	58	theme	calcium	582:588	arg1	polymer					599:605	calcium alginate polymer	582:605	calcium alginate polymer	582:605	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	9	59	from	impeded	1357:1363	arg1	conditions					1376:1385	neutral conditions	1368:1385	neutral conditions	1368:1385	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	1	60	theme	treatment	123:131	arg1	WTRs					144:147	WTRs	144:147	WTRs	144:147	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	1	60	theme	treatment	123:131	arg1	residuals					133:141	Water treatment residuals	117:141	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides,	117:287	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	10	61	theme	adsorption	1719:1728	arg1	effectiveness					1730:1742	similar adsorption effectiveness	1711:1742	similar adsorption effectiveness for both arsenic species	1711:1767	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	1	62	dep	iron	262:265	arg1	oxides					281:286	oxides	281:286	oxides	281:286	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	3	63	theme	alginate	590:597	arg1	polymer					599:605	calcium alginate polymer	582:605	calcium alginate polymer	582:605	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	7	64	theme	acidic	1066:1071	arg1	pH					1085:1086	pH 4.5	1085:1090	pH 4.5	1085:1090	In slightly acidic conditions (pH 4.5), its maximum sorption capacity was 3.4 and 2.9 mg g-1 for As(III) and As(V), respectively.
27164875	7	64	theme	acidic	1066:1071	arg1	conditions					1073:1082	slightly acidic conditions	1057:1082	slightly acidic conditions (pH 4.5)	1057:1091	In slightly acidic conditions (pH 4.5), its maximum sorption capacity was 3.4 and 2.9 mg g-1 for As(III) and As(V), respectively.
27164875	9	65	attach	presence	1309:1316	arg1	chains					1350:1355	polymer chains	1342:1355	polymer chains	1342:1355	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	9	65	attach	presence	1309:1316	arg2	groups					1332:1337	carboxylic groups	1321:1337	carboxylic groups	1321:1337	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	10	66	theme	arsenic	1753:1759	arg1	species					1761:1767	both arsenic species	1748:1767	both arsenic species	1748:1767	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	3	67	theme	sorptive	626:633	arg1	properties					635:644	sorptive properties	626:644	sorptive properties of the obtained composite sorbent toward As(III) and As(V)	626:703	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	6	68	theme	5 	982:983	arg1	residuals					986:994	5 % residuals	982:994	5 % residuals	982:994	The best properties were exhibited by a sorbent containing 5 % residuals, formed in alginate solution with a concentration of 1 %.
27164875	10	69	theme	optimal	1555:1561	arg1	condition					1563:1571	The optimal condition	1551:1571	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent	1551:1677	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	10	69	theme	optimal	1555:1561	arg1	pH					1698:1699	acidic pH	1691:1699	acidic pH	1691:1699	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	10	70	theme	arsenates	1601:1609	arg1	removal					1590:1596	simultaneous removal	1577:1596	simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent	1577:1677	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	6	71	theme	%	984:984	arg1	residuals					986:994	5 % residuals	982:994	5 % residuals	982:994	The best properties were exhibited by a sorbent containing 5 % residuals, formed in alginate solution with a concentration of 1 %.
27164875	0	72	from	studies	108:114	arg1	removal					64:70	arsenic removal	56:70	arsenic removal from water-formation and adsorption studies	56:114	Alginate beads containing water treatment residuals for arsenic removal from water-formation and adsorption studies.
27164875	3	73	theme	properties	635:644	arg1	polymer					599:605	calcium alginate polymer	582:605	calcium alginate polymer	582:605	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	3	73	theme	properties	635:644	arg1	examination					611:621	examination	611:621	examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V)	611:703	The aim of this study was entrapment of chemically pretreated WTR into calcium alginate polymer and examination of sorptive properties of the obtained composite sorbent toward As(III) and As(V).
27164875	2	74	with	difficulties	458:469	arg1	separation					476:485	separation	476:485	separation from treated solutions	476:508	Nonetheless, since they consist of microparticles, their practical use as an adsorbent is limited by difficulties with separation from treated solutions.
27164875	10	75	from	water	1630:1634	arg1	removal					1590:1596	simultaneous removal	1577:1596	simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent	1577:1677	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	1	76	theme	due	239:241	arg1	water					232:236	infiltration water	219:236	infiltration water	219:236	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27164875	10	77	theme	acidic	1691:1696	arg1	condition					1563:1571	The optimal condition	1551:1571	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent	1551:1677	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	10	77	theme	acidic	1691:1696	arg1	pH					1698:1699	acidic pH	1691:1699	acidic pH	1691:1699	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	6	78	theme	%	1051:1051	arg1	concentration					1032:1044	a concentration	1030:1044	a concentration of 1 %	1030:1051	The best properties were exhibited by a sorbent containing 5 % residuals, formed in alginate solution with a concentration of 1 %.
27164875	10	79	theme	simultaneous	1577:1588	arg1	removal					1590:1596	simultaneous removal	1577:1596	simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent	1577:1677	The optimal condition for simultaneous removal of arsenates and arsenites from water by means of the obtained composite sorbent is slightly acidic pH, ensuring similar adsorption effectiveness for both arsenic species.
27164875	9	80	from	presence	1309:1316	arg1	chains					1350:1355	polymer chains	1342:1355	polymer chains	1342:1355	The presence of carboxylic groups in polymer chains impeded in neutral conditions the diffusion of anions into sorbent beads; therefore, the main rate-limiting step of the adsorption, mainly in the case of arsenates, was intraparticle diffusion.
27164875	8	81	theme	neutral	1187:1193	arg1	pH					1195:1196	neutral pH	1187:1196	neutral pH	1187:1196	At neutral pH, the adsorption effectiveness decreased to 3.3 mg As g-1 for arsenites and to 0.7 mg As g-1 for arsenates.
27164875	7	82	theme	maximum	1098:1104	arg1	capacity					1115:1122	its maximum sorption capacity	1094:1122	its maximum sorption capacity	1094:1122	In slightly acidic conditions (pH 4.5), its maximum sorption capacity was 3.4 and 2.9 mg g-1 for As(III) and As(V), respectively.
27164875	1	83	theme	high	246:249	arg1	content					251:257	high content	246:257	high content of iron and manganese oxides	246:286	Water treatment residuals (WTRs) produced in large quantities during deironing and demanganization of infiltration water, due to high content of iron and manganese oxides, exhibit excellent sorptive properties toward arsenate and arsenite.
27732924	1	0	theme	water	223:227	arg1	resources					229:237	water resources	223:237	water resources	223:237	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	4	1	theme	metal-removal	680:692	arg1	conditions					694:703	the best metal-removal conditions	671:703	the best metal-removal conditions	671:703	To determine the best metal-removal conditions, a statistical study using statistical planning was carried out.
27732924	3	2	theme	treatment	505:513	arg1	agent					515:519	a MIW treatment agent	499:519	a MIW treatment agent	499:519	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	3	2	theme	treatment	505:513	arg1	shell					477:481	Shrimp shell	470:481	Shrimp shell	470:481	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	5	3	from	MIW	933:935	arg1	removal					894:900	the removal	890:900	the removal of Fe, Al, Mn, Co, and Ni from MIW	890:935	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
27732924	10	4	theme	experimental	1869:1880	arg1	values					1882:1887	the best experimental values	1860:1887	the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells	1860:1958	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	3	5	theme	low-cost	537:544	arg1	biopolymer					560:569	a low-cost metal-sorbent biopolymer	535:569	a low-cost metal-sorbent biopolymer with a high chitin content	535:596	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	3	5	theme	low-cost	537:544	arg1	it					529:530	it	529:530	it	529:530	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	6	6	theme	rotational	973:982	arg1	design					997:1002	a central composite rotational experimental design	953:1002	a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22)	953:1051	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	6	6	theme	rotational	973:982	arg1	CCRD					1005:1008	CCRD	1005:1008	CCRD	1005:1008	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	1	7	theme	serious	165:171	arg1	problems					180:187	the most serious mining problems	156:187	the most serious mining problems	156:187	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	7	8	theme	variable	1353:1360	arg1	influence					1210:1218	the significant influence	1194:1218	the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal	1194:1428	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	7	8	theme	variable	1353:1360	arg1	p < 0.05					1221:1228	p < 0.05	1221:1228	p < 0.05	1221:1228	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	8	9	theme	95	1525:1526	arg1	%					1527:1527	%	1527:1527	%	1527:1527	Analysis of variance (ANOVA) for Al, Co, and Ni removal showed that the model is valid at the 95% confidence interval and that no adjustment needed within the ranges evaluated of agitation (0-251.5 rpm) and shrimp-shell content (1.2-12.8 g L-1).
27732924	2	10	theme	main	261:264	arg1	characteristics					266:280	The main characteristics	257:280	The main characteristics of MIW	257:287	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	10	theme	main	261:264	arg1	pH					299:300	a low pH	293:300	a low pH (between 2 and 4)	293:318	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	1	11	theme	problems	180:187	arg1	problems					180:187	the most serious mining problems	156:187	the most serious mining problems	156:187	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	1	11	theme	problems	180:187	arg1	one					149:151	one	149:151	one	149:151	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	7	12	theme	shrimp-shell	1332:1343	arg1	variable					1353:1360	the shrimp-shell content variable	1328:1360	the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal	1328:1428	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	10	13	theme	variables	1896:1904	arg1	values					1882:1887	the best experimental values	1860:1887	the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells	1860:1958	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	6	14	theme	composite	963:971	arg1	design					997:1002	a central composite rotational experimental design	953:1002	a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22)	953:1051	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	6	14	theme	composite	963:971	arg1	CCRD					1005:1008	CCRD	1005:1008	CCRD	1005:1008	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	10	15	from	terms	1787:1791	arg1	possible					1838:1845	possible	1838:1845	possible	1838:1845	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	10	15	from	terms	1787:1791	arg1	pollutants					1819:1828	removing pollutants	1810:1828	removing pollutants	1810:1828	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	8	16	theme	confidence	1529:1538	arg1	interval					1540:1547	the 95% confidence interval	1521:1547	the 95% confidence interval	1521:1547	Analysis of variance (ANOVA) for Al, Co, and Ni removal showed that the model is valid at the 95% confidence interval and that no adjustment needed within the ranges evaluated of agitation (0-251.5 rpm) and shrimp-shell content (1.2-12.8 g L-1).
27732924	5	17	theme	shrimp-shell	865:876	arg1	content					878:884	the shrimp-shell content	861:884	the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW	861:935	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
27732924	0	18	theme	statistical	18:28	arg1	design					43:48	the statistical experimental design	14:48	the statistical experimental design	14:48	Aplication of the statistical experimental design to optimize mine-impacted water (MIW) remediation using shrimp-shell.
27732924	9	19	dep	Fe	1751:1752	arg1	removal					1761:1767	removal	1761:1767	removal	1761:1767	The model required adjustments to the 90% and 75% confidence interval for Fe and Mn removal, respectively.
27732924	10	20	from	efficiency	1796:1805	arg1	pollutants					1819:1828	removing pollutants	1810:1828	removing pollutants	1810:1828	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	1	21	from	impact	213:218	arg1	resources					229:237	water resources	223:237	water resources	223:237	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	1	21	from	impact	213:218	arg1	life					251:254	aquatic life	243:254	aquatic life	243:254	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	1	22	theme	Mine-impacted	120:132	arg1	water					134:138	Mine-impacted water	120:138	Mine-impacted water (MIW)	120:144	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	1	22	theme	Mine-impacted	120:132	arg1	MIW					141:143	MIW	141:143	MIW	141:143	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	4	23	theme	statistical	708:718	arg1	study					720:724	a statistical study	706:724	a statistical study using statistical planning	706:751	To determine the best metal-removal conditions, a statistical study using statistical planning was carried out.
27732924	3	24	theme	acid-neutralizing	633:649	arg1	carbonate					619:627	calcium carbonate	611:627	calcium carbonate	611:627	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	3	24	theme	acid-neutralizing	633:649	arg1	agent					651:655	an acid-neutralizing agent	630:655	an acid-neutralizing agent	630:655	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	7	25	dep	Mn	1366:1367	arg1	removal					1422:1428	removal	1422:1428	removal	1422:1428	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	3	26	theme	chitin	583:588	arg1	content					590:596	a high chitin content	576:596	a high chitin content	576:596	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	0	27	theme	mine-impacted	62:74	arg1	remediation					88:98	mine-impacted water (MIW) remediation	62:98	mine-impacted water (MIW) remediation using shrimp-shell	62:117	Aplication of the statistical experimental design to optimize mine-impacted water (MIW) remediation using shrimp-shell.
27732924	5	28	theme	Fe	905:906	arg1	removal					894:900	the removal	890:900	the removal of Fe, Al, Mn, Co, and Ni from MIW	890:935	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
27732924	7	29	theme	quadratic	1291:1299	arg1	form					1301:1304	linear and quadratic form	1280:1304	linear and quadratic form	1280:1304	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	6	30	theme	joint	1078:1082	arg1	influence					1084:1092	the joint influence	1074:1092	the joint influence of two formulation variables-agitation	1074:1131	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	5	31	theme	Al	909:910	arg1	removal					894:900	the removal	890:900	the removal of Fe, Al, Mn, Co, and Ni from MIW	890:935	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
27732924	3	32	with	biopolymer	560:569	arg1	content					590:596	a high chitin content	576:596	a high chitin content	576:596	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	7	33	theme	linear	1280:1285	arg1	form					1301:1304	linear and quadratic form	1280:1304	linear and quadratic form	1280:1304	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	2	34	theme	human	458:462	arg1	life					464:467	human life	458:467	human life	458:467	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	3	35	theme	calcium	611:617	arg1	carbonate					619:627	calcium carbonate	611:627	calcium carbonate	611:627	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	3	35	theme	calcium	611:617	arg1	agent					651:655	an acid-neutralizing agent	630:655	an acid-neutralizing agent	630:655	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	2	36	theme	high	324:327	arg1	concentrations					329:342	high concentrations	324:342	high concentrations	324:342	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	1	37	theme	aquatic	243:249	arg1	life					251:254	aquatic life	243:254	aquatic life	243:254	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	2	38	theme	ions	363:366	arg1	characteristics					266:280	The main characteristics	257:280	The main characteristics of MIW	257:287	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	38	theme	ions	363:366	arg1	pH					299:300	a low pH	293:300	a low pH (between 2 and 4)	293:318	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	38	theme	ions	363:366	arg1	concentrations					329:342	high concentrations	324:342	high concentrations	324:342	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	10	39	from	possible	1838:1845	arg1	terms					1787:1791	terms	1787:1791	terms of efficiency in removing pollutants	1787:1828	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	6	40	theme	shrimp-shell	1141:1152	arg1	content					1154:1160	the shrimp-shell content	1137:1160	the shrimp-shell content	1137:1160	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	0	41	theme	experimental	30:41	arg1	design					43:48	the statistical experimental design	14:48	the statistical experimental design	14:48	Aplication of the statistical experimental design to optimize mine-impacted water (MIW) remediation using shrimp-shell.
27732924	5	42	theme	Mn	913:914	arg1	removal					894:900	the removal	890:900	the removal of Fe, Al, Mn, Co, and Ni from MIW	890:935	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
27732924	7	43	theme	variable	1248:1255	arg1	influence					1210:1218	the significant influence	1194:1218	the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal	1194:1428	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	7	43	theme	variable	1248:1255	arg1	p < 0.05					1221:1228	p < 0.05	1221:1228	p < 0.05	1221:1228	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	6	44	theme	formulation	1101:1111	arg1	variables-agitation					1113:1131	two formulation variables-agitation	1097:1131	two formulation variables-agitation	1097:1131	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	6	45	from	midpoint	1039:1046	arg1	quadruplicate					1018:1030	a quadruplicate	1016:1030	a quadruplicate at the midpoint (22)	1016:1051	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	3	46	theme	Shrimp	470:475	arg1	agent					515:519	a MIW treatment agent	499:519	a MIW treatment agent	499:519	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	3	46	theme	Shrimp	470:475	arg1	shell					477:481	Shrimp shell	470:481	Shrimp shell	470:481	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	3	47	contain	contains	602:609	arg2	carbonate					619:627	calcium carbonate	611:627	calcium carbonate	611:627	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	3	47	contain	contains	602:609	arg1	biopolymer					560:569	a low-cost metal-sorbent biopolymer	535:569	a low-cost metal-sorbent biopolymer with a high chitin content	535:596	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	3	47	contain	contains	602:609	arg2	agent					651:655	an acid-neutralizing agent	630:655	an acid-neutralizing agent	630:655	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	3	47	contain	contains	602:609	arg1	it					529:530	it	529:530	it	529:530	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	1	48	theme	negative	204:211	arg1	impact					213:218	a high negative impact	197:218	a high negative impact on water resources and aquatic life	197:254	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	6	49	used	used	1057:1060	arg2	CCRD					1005:1008	CCRD	1005:1008	CCRD	1005:1008	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	6	49	used	used	1057:1060	arg2	design					997:1002	a central composite rotational experimental design	953:1002	a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22)	953:1051	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	3	50	theme	MIW	501:503	arg1	agent					515:519	a MIW treatment agent	499:519	a MIW treatment agent	499:519	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	3	50	theme	MIW	501:503	arg1	shell					477:481	Shrimp shell	470:481	Shrimp shell	470:481	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	7	51	theme	significant	1198:1208	arg1	influence					1210:1218	the significant influence	1194:1218	the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal	1194:1428	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	7	51	theme	significant	1198:1208	arg1	p < 0.05					1221:1228	p < 0.05	1221:1228	p < 0.05	1221:1228	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	6	52	with	design	997:1002	arg1	quadruplicate					1018:1030	a quadruplicate	1016:1030	a quadruplicate at the midpoint (22)	1016:1051	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	4	53	theme	best	675:678	arg1	conditions					694:703	the best metal-removal conditions	671:703	the best metal-removal conditions	671:703	To determine the best metal-removal conditions, a statistical study using statistical planning was carried out.
27732924	9	54	theme	interval	1738:1745	arg1	%					1725:1725	75%	1723:1725	75% confidence interval for Fe and Mn removal	1723:1767	The model required adjustments to the 90% and 75% confidence interval for Fe and Mn removal, respectively.
27732924	7	55	theme	statistical	1167:1177	arg1	results					1179:1185	The statistical results	1163:1185	The statistical results	1163:1185	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	5	56	theme	Co	917:918	arg1	removal					894:900	the removal	890:900	the removal of Fe, Al, Mn, Co, and Ni from MIW	890:935	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
27732924	6	57	theme	experimental	984:995	arg1	design					997:1002	a central composite rotational experimental design	953:1002	a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22)	953:1051	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	6	57	theme	experimental	984:995	arg1	CCRD					1005:1008	CCRD	1005:1008	CCRD	1005:1008	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	2	58	theme	MIW	285:287	arg1	characteristics					266:280	The main characteristics	257:280	The main characteristics of MIW	257:287	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	58	theme	MIW	285:287	arg1	pH					299:300	a low pH	293:300	a low pH (between 2 and 4)	293:318	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	7	59	theme	content	1345:1351	arg1	variable					1353:1360	the shrimp-shell content variable	1328:1360	the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal	1328:1428	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	10	60	theme	best	1864:1867	arg1	values					1882:1887	the best experimental values	1860:1887	the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells	1860:1958	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	8	61	dep	Co	1468:1469	arg1	Al					1464:1465	Al, Co, and Ni removal	1464:1485	Al	1464:1465	Analysis of variance (ANOVA) for Al, Co, and Ni removal showed that the model is valid at the 95% confidence interval and that no adjustment needed within the ranges evaluated of agitation (0-251.5 rpm) and shrimp-shell content (1.2-12.8 g L-1).
27732924	8	61	dep	Co	1468:1469	arg1	removal					1479:1485	removal	1479:1485	removal	1479:1485	Analysis of variance (ANOVA) for Al, Co, and Ni removal showed that the model is valid at the 95% confidence interval and that no adjustment needed within the ranges evaluated of agitation (0-251.5 rpm) and shrimp-shell content (1.2-12.8 g L-1).
27732924	3	62	theme	metal-sorbent	546:558	arg1	biopolymer					560:569	a low-cost metal-sorbent biopolymer	535:569	a low-cost metal-sorbent biopolymer with a high chitin content	535:596	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	3	62	theme	metal-sorbent	546:558	arg1	it					529:530	it	529:530	it	529:530	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	8	63	theme	%	1527:1527	arg1	interval					1540:1547	the 95% confidence interval	1521:1547	the 95% confidence interval	1521:1547	Analysis of variance (ANOVA) for Al, Co, and Ni removal showed that the model is valid at the 95% confidence interval and that no adjustment needed within the ranges evaluated of agitation (0-251.5 rpm) and shrimp-shell content (1.2-12.8 g L-1).
27732924	1	64	theme	mining	173:178	arg1	problems					180:187	the most serious mining problems	156:187	the most serious mining problems	156:187	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	4	65	theme	statistical	732:742	arg1	planning					744:751	statistical planning	732:751	statistical planning	732:751	To determine the best metal-removal conditions, a statistical study using statistical planning was carried out.
27732924	6	66	theme	central	955:961	arg1	design					997:1002	a central composite rotational experimental design	953:1002	a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22)	953:1051	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	6	66	theme	central	955:961	arg1	CCRD					1005:1008	CCRD	1005:1008	CCRD	1005:1008	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	3	67	theme	high	578:581	arg1	content					590:596	a high chitin content	576:596	a high chitin content	576:596	Shrimp shell was selected as a MIW treatment agent because it is a low-cost metal-sorbent biopolymer with a high chitin content and contains calcium carbonate, an acid-neutralizing agent.
27732924	5	68	theme	content	878:884	arg1	dependence					847:856	dependence	847:856	dependence	847:856	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
27732924	5	68	theme	content	878:884	arg1	influence					833:841	influence	833:841	influence	833:841	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
27732924	5	69	theme	influence	833:841	arg1	degree					823:828	the degree	819:828	the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW	819:935	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
27732924	0	70	theme	water	76:80	arg1	remediation					88:98	mine-impacted water (MIW) remediation	62:98	mine-impacted water (MIW) remediation using shrimp-shell	62:117	Aplication of the statistical experimental design to optimize mine-impacted water (MIW) remediation using shrimp-shell.
27732924	5	71	theme	dependence	847:856	arg1	degree					823:828	the degree	819:828	the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW	819:935	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
27732924	8	72	theme	variance	1443:1450	arg1	Analysis					1431:1438	Analysis	1431:1438	Analysis of variance (ANOVA) for Al, Co, and Ni removal	1431:1485	Analysis of variance (ANOVA) for Al, Co, and Ni removal showed that the model is valid at the 95% confidence interval and that no adjustment needed within the ranges evaluated of agitation (0-251.5 rpm) and shrimp-shell content (1.2-12.8 g L-1).
27732924	7	73	theme	quadratic	1406:1414	arg1	form					1416:1419	linear and quadratic form	1395:1419	linear and quadratic form	1395:1419	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	7	73	theme	quadratic	1406:1414	arg1	Co					1391:1392	Co	1391:1392	Co (linear and quadratic form)	1391:1420	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	10	74	from	pollutants	1819:1828	arg1	terms					1787:1791	terms	1787:1791	terms of efficiency in removing pollutants	1787:1828	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	0	75	theme	MIW	83:85	arg1	remediation					88:98	mine-impacted water (MIW) remediation	62:98	mine-impacted water (MIW) remediation using shrimp-shell	62:117	Aplication of the statistical experimental design to optimize mine-impacted water (MIW) remediation using shrimp-shell.
27732924	7	76	theme	linear	1395:1400	arg1	form					1416:1419	linear and quadratic form	1395:1419	linear and quadratic form	1395:1419	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	7	76	theme	linear	1395:1400	arg1	Co					1391:1392	Co	1391:1392	Co (linear and quadratic form)	1391:1420	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	10	77	theme	shrimp-shells	1946:1958	arg1	9.36 g L-1					1932:1941	9.36 g L-1	1932:1941	9.36 g L-1	1932:1941	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	10	77	theme	shrimp-shells	1946:1958	arg1	188 rpm					1920:1926	188 rpm	1920:1926	188 rpm	1920:1926	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	10	78	theme	removing	1810:1817	arg1	pollutants					1819:1828	removing pollutants	1810:1828	removing pollutants	1810:1828	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	7	79	theme	Ni	1268:1269	arg1	removal					1271:1277	Ni removal	1268:1277	Ni removal	1268:1277	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	10	80	theme	efficiency	1796:1805	arg1	terms					1787:1791	terms	1787:1791	terms of efficiency in removing pollutants	1787:1828	In terms of efficiency in removing pollutants, it was possible to determine the best experimental values of the variables considered as 188 rpm and 9.36 g L-1 of shrimp-shells.
27732924	2	81	dep	ions	363:366	arg1	Fe					389:390	Fe	389:390	Fe	389:390	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	81	dep	ions	363:366	arg1	Al					393:394	Al	393:394	Al	393:394	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	81	dep	ions	363:366	arg1	Mg					405:406	Mg	405:406	Mg	405:406	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	81	dep	ions	363:366	arg1	Cd					369:370	Cd	369:370	Cd	369:370	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	81	dep	ions	363:366	arg1	Pb					381:382	Pb	381:382	Pb	381:382	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	81	dep	ions	363:366	arg1	etc.					409:412	etc.	409:412	etc.	409:412	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	81	dep	ions	363:366	arg1	Zn					385:386	Zn	385:386	Zn	385:386	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	81	dep	ions	363:366	arg1	Cr					397:398	Cr	397:398	Cr	397:398	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	81	dep	ions	363:366	arg1	Cu					373:374	Cu	373:374	Cu	373:374	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	81	dep	ions	363:366	arg1	Mn					401:402	Mn	401:402	Mn	401:402	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	81	dep	ions	363:366	arg1	Ni					377:378	Ni	377:378	Ni	377:378	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	8	82	theme	shrimp-shell	1638:1649	arg1	1.2-12.8 g L-1					1660:1673	1.2-12.8 g L-1	1660:1673	1.2-12.8 g L-1	1660:1673	Analysis of variance (ANOVA) for Al, Co, and Ni removal showed that the model is valid at the 95% confidence interval and that no adjustment needed within the ranges evaluated of agitation (0-251.5 rpm) and shrimp-shell content (1.2-12.8 g L-1).
27732924	8	82	theme	shrimp-shell	1638:1649	arg1	content					1651:1657	shrimp-shell content	1638:1657	shrimp-shell content (1.2-12.8 g L-1)	1638:1674	Analysis of variance (ANOVA) for Al, Co, and Ni removal showed that the model is valid at the 95% confidence interval and that no adjustment needed within the ranges evaluated of agitation (0-251.5 rpm) and shrimp-shell content (1.2-12.8 g L-1).
27732924	5	83	theme	Ni	925:926	arg1	removal					894:900	the removal	890:900	the removal of Fe, Al, Mn, Co, and Ni from MIW	890:935	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
27732924	7	84	theme	linear	1370:1375	arg1	Mn					1366:1367	Mn	1366:1367	Mn (linear form)	1366:1381	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	7	84	theme	linear	1370:1375	arg1	form					1377:1380	linear form	1370:1380	linear form	1370:1380	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	2	85	theme	metal	357:361	arg1	ions					363:366	metal ions	357:366	metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.)	357:413	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	86	theme	SO42-	347:351	arg1	characteristics					266:280	The main characteristics	257:280	The main characteristics of MIW	257:287	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	86	theme	SO42-	347:351	arg1	pH					299:300	a low pH	293:300	a low pH (between 2 and 4)	293:318	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	86	theme	SO42-	347:351	arg1	concentrations					329:342	high concentrations	324:342	high concentrations	324:342	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	0	87	theme	design	43:48	arg1	Aplication					0:9	Aplication	0:9	Aplication of the statistical experimental design	0:48	Aplication of the statistical experimental design to optimize mine-impacted water (MIW) remediation using shrimp-shell.
27732924	7	88	dep	Fe	1261:1262	arg1	form					1301:1304	linear and quadratic form	1280:1304	linear and quadratic form	1280:1304	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	2	89	theme	concentrations	329:342	arg1	many					416:419	many	416:419	many	416:419	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	89	theme	concentrations	329:342	arg1	concentrations					329:342	high concentrations	324:342	high concentrations	324:342	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	6	90	theme	variables-agitation	1113:1131	arg1	influence					1084:1092	the joint influence	1074:1092	the joint influence of two formulation variables-agitation	1074:1131	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	6	90	theme	variables-agitation	1113:1131	arg1	content					1154:1160	the shrimp-shell content	1137:1160	the shrimp-shell content	1137:1160	In this study, a central composite rotational experimental design (CCRD) with a quadruplicate at the midpoint (22) was used to evaluate the joint influence of two formulation variables-agitation and the shrimp-shell content.
27732924	1	91	contain	has	193:195	arg1	water					134:138	Mine-impacted water	120:138	Mine-impacted water (MIW)	120:144	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	1	91	contain	has	193:195	arg2	impact					213:218	a high negative impact	197:218	a high negative impact on water resources and aquatic life	197:254	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	1	91	contain	has	193:195	arg1	MIW					141:143	MIW	141:143	MIW	141:143	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	2	92	theme	low	295:297	arg1	characteristics					266:280	The main characteristics	257:280	The main characteristics of MIW	257:287	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	2	92	theme	low	295:297	arg1	pH					299:300	a low pH	293:300	a low pH (between 2 and 4)	293:318	The main characteristics of MIW are a low pH (between 2 and 4) and high concentrations of SO42- and metal ions (Cd, Cu, Ni, Pb, Zn, Fe, Al, Cr, Mn, Mg, etc.), many of which are toxic to ecosystems and human life.
27732924	7	93	theme	agitation	1238:1246	arg1	variable					1248:1255	the agitation variable	1234:1255	the agitation variable	1234:1255	The statistical results showed the significant influence (p < 0.05) of the agitation variable for Fe and Ni removal (linear and quadratic form, respectively) and of the shrimp-shell content variable for Mn (linear form), Al and Co (linear and quadratic form) removal.
27732924	8	94	from	interval	1540:1547	arg1	valid					1512:1516	valid	1512:1516	valid	1512:1516	Analysis of variance (ANOVA) for Al, Co, and Ni removal showed that the model is valid at the 95% confidence interval and that no adjustment needed within the ranges evaluated of agitation (0-251.5 rpm) and shrimp-shell content (1.2-12.8 g L-1).
27732924	8	94	from	interval	1540:1547	arg1	model					1503:1507	the model	1499:1507	the model	1499:1507	Analysis of variance (ANOVA) for Al, Co, and Ni removal showed that the model is valid at the 95% confidence interval and that no adjustment needed within the ranges evaluated of agitation (0-251.5 rpm) and shrimp-shell content (1.2-12.8 g L-1).
27732924	1	95	theme	high	199:202	arg1	impact					213:218	a high negative impact	197:218	a high negative impact on water resources and aquatic life	197:254	Mine-impacted water (MIW) is one of the most serious mining problems and has a high negative impact on water resources and aquatic life.
27732924	5	96	theme	work	798:801	arg1	objective					780:788	the objective	776:788	the objective of this work	776:801	Thus, the objective of this work was to identify the degree of influence and dependence of the shrimp-shell content for the removal of Fe, Al, Mn, Co, and Ni from MIW.
25157999	0	0	with	effect	93:98	arg1	substrates					154:163	two different substrates	140:163	two different substrates	140:163	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	2	1	theme	several	395:401	arg1	enzymes					403:409	several enzymes	395:409	several enzymes	395:409	Macromolecular crowding and its effect on enzymatic reactions has been studied for several enzymes and is often understood in terms of changes to enzyme conformation.
25157999	1	2	theme	macromolecular	286:299	arg1	cosolutes					301:309	macromolecular cosolutes	286:309	macromolecular cosolutes	286:309	The importance of solution composition on enzymatic reactions is increasingly appreciated, particularly with respect to macromolecular cosolutes.
25157999	3	3	theme	crowded	604:610	arg1	solution					612:619	crowded solution	604:619	crowded solution	604:619	Comparatively little attention has been paid to the chemical properties of small-molecule substrates for enzyme reactions in crowded solution.
25157999	10	4	from	3.9×	1671:1674	arg1	8k					1687:1688	10% PEG 8k	1679:1688	10% PEG 8k	1679:1688	The diffusivity of the TMB decreased 3.9× in 10% PEG 8k compared to that in buffer and decreased only 1.7× for OPD.
25157999	6	5	with	rates	1005:1009	arg1	TMB					1016:1018	TMB	1016:1018	TMB	1016:1018	Reaction rates with TMB were much more sensitive to the presence of crowding agents and cosolutes than OPD, suggesting that the small-molecule substrates may themselves be interacting with crowders and cosolutes.
25157999	2	6	theme	Macromolecular	312:325	arg1	crowding					327:334	Macromolecular crowding	312:334	Macromolecular crowding	312:334	Macromolecular crowding and its effect on enzymatic reactions has been studied for several enzymes and is often understood in terms of changes to enzyme conformation.
25157999	1	7	theme	composition	193:203	arg1	importance					170:179	The importance	166:179	The importance of solution composition on enzymatic reactions	166:226	The importance of solution composition on enzymatic reactions is increasingly appreciated, particularly with respect to macromolecular cosolutes.
25157999	9	8	from	Diffusion	1491:1499	arg1	media					1548:1552	the PEG and dextran reaction media	1519:1552	the PEG and dextran reaction media	1519:1552	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	2	9	theme	enzymatic	354:362	arg1	reactions					364:372	enzymatic reactions	354:372	enzymatic reactions	354:372	Macromolecular crowding and its effect on enzymatic reactions has been studied for several enzymes and is often understood in terms of changes to enzyme conformation.
25157999	1	10	theme	enzymatic	208:216	arg1	reactions					218:226	enzymatic reactions	208:226	enzymatic reactions	208:226	The importance of solution composition on enzymatic reactions is increasingly appreciated, particularly with respect to macromolecular cosolutes.
25157999	6	11	theme	Reaction	996:1003	arg1	rates					1005:1009	Reaction rates	996:1009	Reaction rates with TMB	996:1018	Reaction rates with TMB were much more sensitive to the presence of crowding agents and cosolutes than OPD, suggesting that the small-molecule substrates may themselves be interacting with crowders and cosolutes.
25157999	12	12	theme	multiple	2059:2066	arg1	substrates					2068:2077	multiple substrates	2059:2077	multiple substrates	2059:2077	Because many enzymes can act on multiple substrates, it is important to consider substrate chemistry in understanding enzymatic reactions in complex media such as biological fluids.
25157999	10	13	theme	10	1679:1680	arg1	%					1681:1681	%	1681:1681	%	1681:1681	The diffusivity of the TMB decreased 3.9× in 10% PEG 8k compared to that in buffer and decreased only 1.7× for OPD.
25157999	11	14	theme	molecules	2007:2015	arg1	identity					1991:1998	the identity	1987:1998	the identity of the molecules involved	1987:2024	Together, these data suggest that weak attractive interactions between small-molecule substrates and crowders or cosolutes can reduce substrate chemical activity and consequently decrease enzyme activity and that these effects vary with the identity of the molecules involved.
25157999	0	15	theme	different	144:152	arg1	substrates					154:163	two different substrates	140:163	two different substrates	140:163	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	8	16	theme	crowding	1437:1444	arg1	agents					1446:1451	crowding agents	1437:1451	crowding agents	1437:1451	Even at lower concentrations, Michaelis constants (KM) for HRP with the more hydrophobic substrate increased in the presence of crowding agents and cosolutes, particularly with PEG.
25157999	2	17	from	crowding	327:334	arg1	reactions					364:372	enzymatic reactions	354:372	enzymatic reactions	354:372	Macromolecular crowding and its effect on enzymatic reactions has been studied for several enzymes and is often understood in terms of changes to enzyme conformation.
25157999	11	18	theme	substrate	1884:1892	arg1	activity					1903:1910	substrate chemical activity	1884:1910	substrate chemical activity	1884:1910	Together, these data suggest that weak attractive interactions between small-molecule substrates and crowders or cosolutes can reduce substrate chemical activity and consequently decrease enzyme activity and that these effects vary with the identity of the molecules involved.
25157999	0	19	theme	small-molecule	66:79	arg1	substrates					81:90	small-molecule substrates	66:90	small-molecule substrates	66:90	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	9	20	theme	TMB	1504:1506	arg1	Diffusion					1491:1499	Diffusion	1491:1499	Diffusion of TMB and OPD in the PEG and dextran reaction media	1491:1552	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	3	21	from	reactions	591:599	arg1	solution					612:619	crowded solution	604:619	crowded solution	604:619	Comparatively little attention has been paid to the chemical properties of small-molecule substrates for enzyme reactions in crowded solution.
25157999	5	22	theme	HRP	828:830	arg1	activity					832:839	HRP activity	828:839	HRP activity	828:839	Crowding agents and cosolutes had quite different effects on HRP activity when the substrate used was 3,3',5,5'-tetramethylbenzidine (TMB, which is hydrophobic) as compared to o-phenylenediamine (OPD, which is more hydrophilic).
25157999	1	23	from	importance	170:179	arg1	reactions					218:226	enzymatic reactions	208:226	enzymatic reactions	208:226	The importance of solution composition on enzymatic reactions is increasingly appreciated, particularly with respect to macromolecular cosolutes.
25157999	9	24	theme	OPD	1512:1514	arg1	Diffusion					1491:1499	Diffusion	1491:1499	Diffusion of TMB and OPD in the PEG and dextran reaction media	1491:1552	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	8	25	theme	Michaelis	1339:1347	arg1	constants					1349:1357	Michaelis constants	1339:1357	Michaelis constants (KM) for HRP with the more hydrophobic substrate	1339:1406	Even at lower concentrations, Michaelis constants (KM) for HRP with the more hydrophobic substrate increased in the presence of crowding agents and cosolutes, particularly with PEG.
25157999	8	25	theme	Michaelis	1339:1347	arg1	KM					1360:1361	KM	1360:1361	KM	1360:1361	Even at lower concentrations, Michaelis constants (KM) for HRP with the more hydrophobic substrate increased in the presence of crowding agents and cosolutes, particularly with PEG.
25157999	5	26	contain	had	797:799	arg2	effects					817:823	quite different effects	801:823	quite different effects	801:823	Crowding agents and cosolutes had quite different effects on HRP activity when the substrate used was 3,3',5,5'-tetramethylbenzidine (TMB, which is hydrophobic) as compared to o-phenylenediamine (OPD, which is more hydrophilic).
25157999	5	26	contain	had	797:799	arg1	cosolutes					787:795	cosolutes	787:795	cosolutes	787:795	Crowding agents and cosolutes had quite different effects on HRP activity when the substrate used was 3,3',5,5'-tetramethylbenzidine (TMB, which is hydrophobic) as compared to o-phenylenediamine (OPD, which is more hydrophilic).
25157999	5	26	contain	had	797:799	arg1	agents					776:781	Crowding agents	767:781	Crowding agents	767:781	Crowding agents and cosolutes had quite different effects on HRP activity when the substrate used was 3,3',5,5'-tetramethylbenzidine (TMB, which is hydrophobic) as compared to o-phenylenediamine (OPD, which is more hydrophilic).
25157999	11	27	theme	chemical	1894:1901	arg1	activity					1903:1910	substrate chemical activity	1884:1910	substrate chemical activity	1884:1910	Together, these data suggest that weak attractive interactions between small-molecule substrates and crowders or cosolutes can reduce substrate chemical activity and consequently decrease enzyme activity and that these effects vary with the identity of the molecules involved.
25157999	5	28	dep	o-phenylenediamine	943:960	arg1	OPD					963:965	OPD	963:965	OPD	963:965	Crowding agents and cosolutes had quite different effects on HRP activity when the substrate used was 3,3',5,5'-tetramethylbenzidine (TMB, which is hydrophobic) as compared to o-phenylenediamine (OPD, which is more hydrophilic).
25157999	0	29	theme	peroxidase	115:124	arg1	activity					126:133	horseradish peroxidase activity	103:133	horseradish peroxidase activity	103:133	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	9	30	theme	reaction	1539:1546	arg1	media					1548:1552	the PEG and dextran reaction media	1519:1552	the PEG and dextran reaction media	1519:1552	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	2	31	theme	enzyme	458:463	arg1	conformation					465:476	enzyme conformation	458:476	enzyme conformation	458:476	Macromolecular crowding and its effect on enzymatic reactions has been studied for several enzymes and is often understood in terms of changes to enzyme conformation.
25157999	0	32	theme	macromolecular	16:29	arg1	agents					40:45	macromolecular crowding agents	16:45	macromolecular crowding agents	16:45	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	6	33	theme	crowding	1064:1071	arg1	agents					1073:1078	crowding agents	1064:1078	crowding agents	1064:1078	Reaction rates with TMB were much more sensitive to the presence of crowding agents and cosolutes than OPD, suggesting that the small-molecule substrates may themselves be interacting with crowders and cosolutes.
25157999	12	34	theme	enzymatic	2145:2153	arg1	reactions					2155:2163	enzymatic reactions	2145:2163	enzymatic reactions	2145:2163	Because many enzymes can act on multiple substrates, it is important to consider substrate chemistry in understanding enzymatic reactions in complex media such as biological fluids.
25157999	2	35	theme	changes	447:453	arg1	terms					438:442	terms	438:442	terms of changes to enzyme conformation	438:476	Macromolecular crowding and its effect on enzymatic reactions has been studied for several enzymes and is often understood in terms of changes to enzyme conformation.
25157999	0	36	theme	agents	40:45	arg1	Interactions					0:11	Interactions	0:11	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.	0:164	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	8	37	theme	hydrophobic	1386:1396	arg1	substrate					1398:1406	the more hydrophobic substrate	1377:1406	the more hydrophobic substrate	1377:1406	Even at lower concentrations, Michaelis constants (KM) for HRP with the more hydrophobic substrate increased in the presence of crowding agents and cosolutes, particularly with PEG.
25157999	11	38	theme	weak	1784:1787	arg1	interactions					1800:1811	weak attractive interactions	1784:1811	weak attractive interactions between small-molecule substrates and crowders or cosolutes	1784:1871	Together, these data suggest that weak attractive interactions between small-molecule substrates and crowders or cosolutes can reduce substrate chemical activity and consequently decrease enzyme activity and that these effects vary with the identity of the molecules involved.
25157999	0	39	theme	cosolutes	51:59	arg1	Interactions					0:11	Interactions	0:11	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.	0:164	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	9	40	theme	field	1581:1585	arg1	PFG-NMR					1624:1630	PFG-NMR	1624:1630	PFG-NMR	1624:1630	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	9	40	theme	field	1581:1585	arg1	resonance					1613:1621	pulsed field gradient nuclear magnetic resonance	1574:1621	pulsed field gradient nuclear magnetic resonance (PFG-NMR)	1574:1631	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	12	41	theme	substrate	2108:2116	arg1	chemistry					2118:2126	substrate chemistry	2108:2126	substrate chemistry	2108:2126	Because many enzymes can act on multiple substrates, it is important to consider substrate chemistry in understanding enzymatic reactions in complex media such as biological fluids.
25157999	0	42	with	Interactions	0:11	arg1	substrates					81:90	small-molecule substrates	66:90	small-molecule substrates	66:90	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	9	43	theme	nuclear	1596:1602	arg1	PFG-NMR					1624:1630	PFG-NMR	1624:1630	PFG-NMR	1624:1630	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	9	43	theme	nuclear	1596:1602	arg1	resonance					1613:1621	pulsed field gradient nuclear magnetic resonance	1574:1621	pulsed field gradient nuclear magnetic resonance (PFG-NMR)	1574:1631	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	6	44	theme	small-molecule	1124:1137	arg1	substrates					1139:1148	the small-molecule substrates	1120:1148	the small-molecule substrates	1120:1148	Reaction rates with TMB were much more sensitive to the presence of crowding agents and cosolutes than OPD, suggesting that the small-molecule substrates may themselves be interacting with crowders and cosolutes.
25157999	11	45	theme	small-molecule	1821:1834	arg1	substrates					1836:1845	small-molecule substrates	1821:1845	small-molecule substrates	1821:1845	Together, these data suggest that weak attractive interactions between small-molecule substrates and crowders or cosolutes can reduce substrate chemical activity and consequently decrease enzyme activity and that these effects vary with the identity of the molecules involved.
25157999	0	46	dep	Interactions	0:11	arg1	effect					93:98	effect	93:98	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.	0:164	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	10	47	theme	%	1681:1681	arg1	8k					1687:1688	10% PEG 8k	1679:1688	10% PEG 8k	1679:1688	The diffusivity of the TMB decreased 3.9× in 10% PEG 8k compared to that in buffer and decreased only 1.7× for OPD.
25157999	7	48	theme	wt/wt	1265:1269	arg1	%					1271:1271	25-30 wt/wt %	1259:1271	25-30 wt/wt %	1259:1271	At high polyethylene glycol (PEG) concentrations (25-30 wt/wt %), no reaction was observed for TMB.
25157999	7	48	theme	wt/wt	1265:1269	arg1	concentrations					1243:1256	high polyethylene glycol (PEG) concentrations	1212:1256	high polyethylene glycol (PEG) concentrations (25-30 wt/wt %)	1212:1272	At high polyethylene glycol (PEG) concentrations (25-30 wt/wt %), no reaction was observed for TMB.
25157999	3	49	theme	chemical	531:538	arg1	properties					540:549	the chemical properties	527:549	the chemical properties of small-molecule substrates for enzyme reactions in crowded solution	527:619	Comparatively little attention has been paid to the chemical properties of small-molecule substrates for enzyme reactions in crowded solution.
25157999	3	50	theme	enzyme	584:589	arg1	reactions					591:599	enzyme reactions	584:599	enzyme reactions in crowded solution	584:619	Comparatively little attention has been paid to the chemical properties of small-molecule substrates for enzyme reactions in crowded solution.
25157999	1	51	theme	solution	184:191	arg1	composition					193:203	solution composition	184:203	solution composition	184:203	The importance of solution composition on enzymatic reactions is increasingly appreciated, particularly with respect to macromolecular cosolutes.
25157999	4	52	theme	small-molecule	704:717	arg1	substrates					719:728	two small-molecule substrates	700:728	two small-molecule substrates that differ in their hydrophobicity	700:764	In this article, we studied the reaction of horseradish peroxidase (HRP) with two small-molecule substrates that differ in their hydrophobicity.
25157999	0	53	from	effect	93:98	arg1	activity					126:133	horseradish peroxidase activity	103:133	horseradish peroxidase activity	103:133	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	4	54	theme	peroxidase	678:687	arg1	reaction					654:661	the reaction	650:661	the reaction of horseradish peroxidase (HRP) with two small-molecule substrates that differ in their hydrophobicity	650:764	In this article, we studied the reaction of horseradish peroxidase (HRP) with two small-molecule substrates that differ in their hydrophobicity.
25157999	5	55	theme	Crowding	767:774	arg1	agents					776:781	Crowding agents	767:781	Crowding agents	767:781	Crowding agents and cosolutes had quite different effects on HRP activity when the substrate used was 3,3',5,5'-tetramethylbenzidine (TMB, which is hydrophobic) as compared to o-phenylenediamine (OPD, which is more hydrophilic).
25157999	8	56	theme	agents	1446:1451	arg1	presence					1425:1432	the presence	1421:1432	the presence of crowding agents and cosolutes	1421:1465	Even at lower concentrations, Michaelis constants (KM) for HRP with the more hydrophobic substrate increased in the presence of crowding agents and cosolutes, particularly with PEG.
25157999	7	57	theme	glycol	1230:1235	arg1	%					1271:1271	25-30 wt/wt %	1259:1271	25-30 wt/wt %	1259:1271	At high polyethylene glycol (PEG) concentrations (25-30 wt/wt %), no reaction was observed for TMB.
25157999	7	57	theme	glycol	1230:1235	arg1	concentrations					1243:1256	high polyethylene glycol (PEG) concentrations	1212:1256	high polyethylene glycol (PEG) concentrations (25-30 wt/wt %)	1212:1272	At high polyethylene glycol (PEG) concentrations (25-30 wt/wt %), no reaction was observed for TMB.
25157999	7	58	located	observed	1291:1298	arg1	%					1271:1271	25-30 wt/wt %	1259:1271	25-30 wt/wt %	1259:1271	At high polyethylene glycol (PEG) concentrations (25-30 wt/wt %), no reaction was observed for TMB.
25157999	7	58	located	observed	1291:1298	arg2	reaction					1278:1285	no reaction	1275:1285	no reaction	1275:1285	At high polyethylene glycol (PEG) concentrations (25-30 wt/wt %), no reaction was observed for TMB.
25157999	7	58	located	observed	1291:1298	arg1	concentrations					1243:1256	high polyethylene glycol (PEG) concentrations	1212:1256	high polyethylene glycol (PEG) concentrations (25-30 wt/wt %)	1212:1272	At high polyethylene glycol (PEG) concentrations (25-30 wt/wt %), no reaction was observed for TMB.
25157999	4	59	with	reaction	654:661	arg1	substrates					719:728	two small-molecule substrates	700:728	two small-molecule substrates that differ in their hydrophobicity	700:764	In this article, we studied the reaction of horseradish peroxidase (HRP) with two small-molecule substrates that differ in their hydrophobicity.
25157999	8	60	theme	cosolutes	1457:1465	arg1	presence					1425:1432	the presence	1421:1432	the presence of crowding agents and cosolutes	1421:1465	Even at lower concentrations, Michaelis constants (KM) for HRP with the more hydrophobic substrate increased in the presence of crowding agents and cosolutes, particularly with PEG.
25157999	12	61	theme	many	2035:2038	arg1	enzymes					2040:2046	many enzymes	2035:2046	many enzymes	2035:2046	Because many enzymes can act on multiple substrates, it is important to consider substrate chemistry in understanding enzymatic reactions in complex media such as biological fluids.
25157999	8	62	with	constants	1349:1357	arg1	substrate					1398:1406	the more hydrophobic substrate	1377:1406	the more hydrophobic substrate	1377:1406	Even at lower concentrations, Michaelis constants (KM) for HRP with the more hydrophobic substrate increased in the presence of crowding agents and cosolutes, particularly with PEG.
25157999	5	63	theme	different	807:815	arg1	effects					817:823	quite different effects	801:823	quite different effects	801:823	Crowding agents and cosolutes had quite different effects on HRP activity when the substrate used was 3,3',5,5'-tetramethylbenzidine (TMB, which is hydrophobic) as compared to o-phenylenediamine (OPD, which is more hydrophilic).
25157999	11	64	theme	enzyme	1938:1943	arg1	activity					1945:1952	enzyme activity	1938:1952	enzyme activity	1938:1952	Together, these data suggest that weak attractive interactions between small-molecule substrates and crowders or cosolutes can reduce substrate chemical activity and consequently decrease enzyme activity and that these effects vary with the identity of the molecules involved.
25157999	2	65	from	effect	344:349	arg1	reactions					364:372	enzymatic reactions	354:372	enzymatic reactions	354:372	Macromolecular crowding and its effect on enzymatic reactions has been studied for several enzymes and is often understood in terms of changes to enzyme conformation.
25157999	8	66	theme	lower	1317:1321	arg1	concentrations					1323:1336	lower concentrations	1317:1336	lower concentrations	1317:1336	Even at lower concentrations, Michaelis constants (KM) for HRP with the more hydrophobic substrate increased in the presence of crowding agents and cosolutes, particularly with PEG.
25157999	0	67	theme	horseradish	103:113	arg1	peroxidase					115:124	horseradish peroxidase	103:124	horseradish peroxidase activity	103:133	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	12	68	theme	biological	2190:2199	arg1	fluids					2201:2206	biological fluids	2190:2206	biological fluids	2190:2206	Because many enzymes can act on multiple substrates, it is important to consider substrate chemistry in understanding enzymatic reactions in complex media such as biological fluids.
25157999	7	69	theme	high	1212:1215	arg1	%					1271:1271	25-30 wt/wt %	1259:1271	25-30 wt/wt %	1259:1271	At high polyethylene glycol (PEG) concentrations (25-30 wt/wt %), no reaction was observed for TMB.
25157999	7	69	theme	high	1212:1215	arg1	concentrations					1243:1256	high polyethylene glycol (PEG) concentrations	1212:1256	high polyethylene glycol (PEG) concentrations (25-30 wt/wt %)	1212:1272	At high polyethylene glycol (PEG) concentrations (25-30 wt/wt %), no reaction was observed for TMB.
25157999	9	70	theme	PEG	1523:1525	arg1	media					1548:1552	the PEG and dextran reaction media	1519:1552	the PEG and dextran reaction media	1519:1552	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	3	71	theme	little	493:498	arg1	attention					500:508	Comparatively little attention	479:508	Comparatively little attention	479:508	Comparatively little attention has been paid to the chemical properties of small-molecule substrates for enzyme reactions in crowded solution.
25157999	9	72	theme	dextran	1531:1537	arg1	reaction					1539:1546	dextran reaction	1531:1546	dextran reaction	1531:1546	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	6	73	theme	cosolutes	1084:1092	arg1	presence					1052:1059	the presence	1048:1059	the presence of crowding agents and cosolutes	1048:1092	Reaction rates with TMB were much more sensitive to the presence of crowding agents and cosolutes than OPD, suggesting that the small-molecule substrates may themselves be interacting with crowders and cosolutes.
25157999	12	74	theme	complex	2168:2174	arg1	fluids					2201:2206	biological fluids	2190:2206	biological fluids	2190:2206	Because many enzymes can act on multiple substrates, it is important to consider substrate chemistry in understanding enzymatic reactions in complex media such as biological fluids.
25157999	12	74	theme	complex	2168:2174	arg1	media					2176:2180	complex media	2168:2180	complex media such as biological fluids	2168:2206	Because many enzymes can act on multiple substrates, it is important to consider substrate chemistry in understanding enzymatic reactions in complex media such as biological fluids.
25157999	0	75	theme	crowding	31:38	arg1	agents					40:45	macromolecular crowding agents	16:45	macromolecular crowding agents	16:45	Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates.
25157999	6	76	theme	agents	1073:1078	arg1	presence					1052:1059	the presence	1048:1059	the presence of crowding agents and cosolutes	1048:1092	Reaction rates with TMB were much more sensitive to the presence of crowding agents and cosolutes than OPD, suggesting that the small-molecule substrates may themselves be interacting with crowders and cosolutes.
25157999	3	77	theme	substrates	569:578	arg1	properties					540:549	the chemical properties	527:549	the chemical properties of small-molecule substrates for enzyme reactions in crowded solution	527:619	Comparatively little attention has been paid to the chemical properties of small-molecule substrates for enzyme reactions in crowded solution.
25157999	7	78	theme	polyethylene	1217:1228	arg1	glycol					1230:1235	polyethylene glycol	1217:1235	high polyethylene glycol (PEG) concentrations (25-30 wt/wt %)	1212:1272	At high polyethylene glycol (PEG) concentrations (25-30 wt/wt %), no reaction was observed for TMB.
25157999	7	78	theme	polyethylene	1217:1228	arg1	PEG					1238:1240	PEG	1238:1240	PEG	1238:1240	At high polyethylene glycol (PEG) concentrations (25-30 wt/wt %), no reaction was observed for TMB.
25157999	9	79	theme	pulsed	1574:1579	arg1	PFG-NMR					1624:1630	PFG-NMR	1624:1630	PFG-NMR	1624:1630	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	9	79	theme	pulsed	1574:1579	arg1	resonance					1613:1621	pulsed field gradient nuclear magnetic resonance	1574:1621	pulsed field gradient nuclear magnetic resonance (PFG-NMR)	1574:1631	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	3	80	theme	small-molecule	554:567	arg1	substrates					569:578	small-molecule substrates	554:578	small-molecule substrates for enzyme reactions in crowded solution	554:619	Comparatively little attention has been paid to the chemical properties of small-molecule substrates for enzyme reactions in crowded solution.
25157999	11	81	theme	attractive	1789:1798	arg1	interactions					1800:1811	weak attractive interactions	1784:1811	weak attractive interactions between small-molecule substrates and crowders or cosolutes	1784:1871	Together, these data suggest that weak attractive interactions between small-molecule substrates and crowders or cosolutes can reduce substrate chemical activity and consequently decrease enzyme activity and that these effects vary with the identity of the molecules involved.
25157999	9	82	theme	gradient	1587:1594	arg1	PFG-NMR					1624:1630	PFG-NMR	1624:1630	PFG-NMR	1624:1630	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	9	82	theme	gradient	1587:1594	arg1	resonance					1613:1621	pulsed field gradient nuclear magnetic resonance	1574:1621	pulsed field gradient nuclear magnetic resonance (PFG-NMR)	1574:1631	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	10	83	theme	TMB	1657:1659	arg1	diffusivity					1638:1648	The diffusivity	1634:1648	The diffusivity of the TMB	1634:1659	The diffusivity of the TMB decreased 3.9× in 10% PEG 8k compared to that in buffer and decreased only 1.7× for OPD.
25157999	9	84	theme	magnetic	1604:1611	arg1	PFG-NMR					1624:1630	PFG-NMR	1624:1630	PFG-NMR	1624:1630	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	9	84	theme	magnetic	1604:1611	arg1	resonance					1613:1621	pulsed field gradient nuclear magnetic resonance	1574:1621	pulsed field gradient nuclear magnetic resonance (PFG-NMR)	1574:1631	Diffusion of TMB and OPD in the PEG and dextran reaction media was evaluated using pulsed field gradient nuclear magnetic resonance (PFG-NMR).
25157999	4	85	theme	horseradish	666:676	arg1	HRP					690:692	HRP	690:692	HRP	690:692	In this article, we studied the reaction of horseradish peroxidase (HRP) with two small-molecule substrates that differ in their hydrophobicity.
25157999	4	85	theme	horseradish	666:676	arg1	peroxidase					678:687	horseradish peroxidase	666:687	horseradish peroxidase (HRP)	666:693	In this article, we studied the reaction of horseradish peroxidase (HRP) with two small-molecule substrates that differ in their hydrophobicity.
25157999	5	86	dep	5,5'-tetramethylbenzidine	874:898	arg1	TMB					901:903	TMB	901:903	TMB	901:903	Crowding agents and cosolutes had quite different effects on HRP activity when the substrate used was 3,3',5,5'-tetramethylbenzidine (TMB, which is hydrophobic) as compared to o-phenylenediamine (OPD, which is more hydrophilic).
25157999	10	87	theme	PEG	1683:1685	arg1	8k					1687:1688	10% PEG 8k	1679:1688	10% PEG 8k	1679:1688	The diffusivity of the TMB decreased 3.9× in 10% PEG 8k compared to that in buffer and decreased only 1.7× for OPD.
25347830	6	0	theme	crude	616:620	arg1	contents					638:645	the corrected crude and net protein contents	602:645	the corrected crude and net protein contents	602:645	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	7	1	theme	amino	781:785	arg1	acid					787:790	the protein digestibility-corrected amino acid	745:790	the protein digestibility-corrected amino acid score	745:796	In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content.
25347830	0	2	theme	parallela	77:85	arg1	composition					12:22	Nutritional composition	0:22	Nutritional composition	0:22	Nutritional composition and protein quality of the edible beetle Holotrichia parallela.
25347830	0	2	theme	parallela	77:85	arg1	quality					36:42	protein quality	28:42	protein quality	28:42	Nutritional composition and protein quality of the edible beetle Holotrichia parallela.
25347830	4	3	theme	abundant	456:463	arg1	acid					419:422	Oleic acid	413:422	Oleic acid	413:422	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	4	3	theme	abundant	456:463	arg1	acid					437:440	linoleic acid	428:440	linoleic acid	428:440	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	4	3	theme	abundant	456:463	arg1	acids					471:475	the most abundant fatty acids	447:475	the most abundant fatty acids	447:475	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	8	4	theme	proteins	887:894	arg1	source					877:882	a potential source	865:882	a potential source of proteins and minerals for humans and animals	865:930	Adult H. parallela may be a potential source of proteins and minerals for humans and animals.
25347830	8	4	theme	proteins	887:894	arg1	parallela					848:856	Adult H. parallela	839:856	Adult H. parallela	839:856	Adult H. parallela may be a potential source of proteins and minerals for humans and animals.
25347830	2	5	from	minerals	300:307	arg1	high					274:277	high	274:277	high	274:277	Based on nutritional analyses, adult H. parallela is high in protein (70%) and minerals and low in fat.
25347830	6	6	theme	limiting	680:687	arg1	acid					695:698	the limiting amino acid	676:698	the limiting amino acid	676:698	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	6	6	theme	limiting	680:687	arg1	threonine					662:670	threonine	662:670	threonine	662:670	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	8	7	theme	potential	867:875	arg1	source					877:882	a potential source	865:882	a potential source of proteins and minerals for humans and animals	865:930	Adult H. parallela may be a potential source of proteins and minerals for humans and animals.
25347830	8	7	theme	potential	867:875	arg1	parallela					848:856	Adult H. parallela	839:856	Adult H. parallela	839:856	Adult H. parallela may be a potential source of proteins and minerals for humans and animals.
25347830	7	8	from	%	738:738	arg1	content					830:836	the net protein content	814:836	the net protein content	814:836	In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content.
25347830	7	9	theme	protein	822:828	arg1	content					830:836	the net protein content	814:836	the net protein content	814:836	In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content.
25347830	1	10	dep	Motschulsky	134:144	arg1	Coleoptera					147:156	Coleoptera	147:156	Coleoptera	147:156	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea) represents a traditional food source in China.
25347830	7	11	theme	protein	749:755	arg1	acid					787:790	the protein digestibility-corrected amino acid	745:790	the protein digestibility-corrected amino acid score	745:796	In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content.
25347830	8	12	theme	minerals	900:907	arg1	source					877:882	a potential source	865:882	a potential source of proteins and minerals for humans and animals	865:930	Adult H. parallela may be a potential source of proteins and minerals for humans and animals.
25347830	8	12	theme	minerals	900:907	arg1	parallela					848:856	Adult H. parallela	839:856	Adult H. parallela	839:856	Adult H. parallela may be a potential source of proteins and minerals for humans and animals.
25347830	2	13	from	low	313:315	arg1	fat					320:322	fat	320:322	fat	320:322	Based on nutritional analyses, adult H. parallela is high in protein (70%) and minerals and low in fat.
25347830	6	14	theme	corrected	606:614	arg1	contents					638:645	the corrected crude and net protein contents	602:645	the corrected crude and net protein contents	602:645	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	7	15	from	based	805:809	arg1	content					830:836	the net protein content	814:836	the net protein content	814:836	In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content.
25347830	3	16	dep	%	364:364	arg1	10					362:363	10	362:363	10	362:363	H. parallela contained approximately 10% chitin; the corrected protein content was 66%.
25347830	5	17	theme	essential	531:539	arg1	acids					547:551	essential amino acids	531:551	essential amino acids	531:551	Of the total amino acids in H. parallela, 47.4% were essential amino acids.
25347830	3	18	theme	corrected	378:386	arg1	content					396:402	the corrected protein content	374:402	the corrected protein content	374:402	H. parallela contained approximately 10% chitin; the corrected protein content was 66%.
25347830	3	18	theme	corrected	378:386	arg1	%					410:410	66%	408:410	66%	408:410	H. parallela contained approximately 10% chitin; the corrected protein content was 66%.
25347830	3	19	dep	chitin	366:371	arg1	%					364:364	%	364:364	%	364:364	H. parallela contained approximately 10% chitin; the corrected protein content was 66%.
25347830	2	20	from	fat	320:322	arg1	low					313:315	low	313:315	low	313:315	Based on nutritional analyses, adult H. parallela is high in protein (70%) and minerals and low in fat.
25347830	4	21	theme	linoleic	428:435	arg1	acid					419:422	Oleic acid	413:422	Oleic acid	413:422	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	4	21	theme	linoleic	428:435	arg1	acid					437:440	linoleic acid	428:440	linoleic acid	428:440	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	4	21	theme	linoleic	428:435	arg1	acids					471:475	the most abundant fatty acids	447:475	the most abundant fatty acids	447:475	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	7	22	theme	net	818:820	arg1	content					830:836	the net protein content	814:836	the net protein content	814:836	In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content.
25347830	1	23	theme	traditional	187:197	arg1	source					204:209	a traditional food source	185:209	a traditional food source in China	185:218	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea) represents a traditional food source in China.
25347830	0	24	theme	Nutritional	0:10	arg1	composition					12:22	Nutritional composition	0:22	Nutritional composition	0:22	Nutritional composition and protein quality of the edible beetle Holotrichia parallela.
25347830	8	25	theme	H.	845:846	arg1	source					877:882	a potential source	865:882	a potential source of proteins and minerals for humans and animals	865:930	Adult H. parallela may be a potential source of proteins and minerals for humans and animals.
25347830	8	25	theme	H.	845:846	arg1	parallela					848:856	Adult H. parallela	839:856	Adult H. parallela	839:856	Adult H. parallela may be a potential source of proteins and minerals for humans and animals.
25347830	1	26	theme	adult	92:96	arg1	beetle					105:110	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea)	88:172	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea)	88:172	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea) represents a traditional food source in China.
25347830	1	27	theme	food	199:202	arg1	source					204:209	a traditional food source	185:209	a traditional food source in China	185:218	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea) represents a traditional food source in China.
25347830	0	28	theme	protein	28:34	arg1	quality					36:42	protein quality	28:42	protein quality	28:42	Nutritional composition and protein quality of the edible beetle Holotrichia parallela.
25347830	7	29	theme	In	701:702	arg1	digestibility					718:730	In vitro protein digestibility	701:730	In vitro protein digestibility	701:730	In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content.
25347830	6	30	theme	amino	689:693	arg1	acid					695:698	the limiting amino acid	676:698	the limiting amino acid	676:698	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	6	30	theme	amino	689:693	arg1	threonine					662:670	threonine	662:670	threonine	662:670	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	1	31	dep	Coleoptera	147:156	arg1	Scarabaeoidea					159:171	Scarabaeoidea	159:171	Scarabaeoidea	159:171	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea) represents a traditional food source in China.
25347830	5	32	theme	amino	491:495	arg1	acids					497:501	the total amino acids	481:501	the total amino acids in H. parallela	481:517	Of the total amino acids in H. parallela, 47.4% were essential amino acids.
25347830	7	33	theme	protein	710:716	arg1	digestibility					718:730	In vitro protein digestibility	701:730	In vitro protein digestibility	701:730	In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content.
25347830	5	34	from	acids	497:501	arg1	parallela					509:517	parallela	509:517	parallela	509:517	Of the total amino acids in H. parallela, 47.4% were essential amino acids.
25347830	5	35	theme	amino	541:545	arg1	acids					547:551	essential amino acids	531:551	essential amino acids	531:551	Of the total amino acids in H. parallela, 47.4% were essential amino acids.
25347830	3	36	theme	protein	388:394	arg1	content					396:402	the corrected protein content	374:402	the corrected protein content	374:402	H. parallela contained approximately 10% chitin; the corrected protein content was 66%.
25347830	3	36	theme	protein	388:394	arg1	%					410:410	66%	408:410	66%	408:410	H. parallela contained approximately 10% chitin; the corrected protein content was 66%.
25347830	4	37	theme	Oleic	413:417	arg1	acid					419:422	Oleic acid	413:422	Oleic acid	413:422	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	4	37	theme	Oleic	413:417	arg1	acid					437:440	linoleic acid	428:440	linoleic acid	428:440	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	4	37	theme	Oleic	413:417	arg1	acids					471:475	the most abundant fatty acids	447:475	the most abundant fatty acids	447:475	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	2	38	theme	nutritional	230:240	arg1	analyses					242:249	nutritional analyses	230:249	nutritional analyses	230:249	Based on nutritional analyses, adult H. parallela is high in protein (70%) and minerals and low in fat.
25347830	0	39	theme	edible	51:56	arg1	parallela					77:85	the edible beetle Holotrichia parallela	47:85	the edible beetle Holotrichia parallela	47:85	Nutritional composition and protein quality of the edible beetle Holotrichia parallela.
25347830	4	40	theme	fatty	465:469	arg1	acid					419:422	Oleic acid	413:422	Oleic acid	413:422	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	4	40	theme	fatty	465:469	arg1	acid					437:440	linoleic acid	428:440	linoleic acid	428:440	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	4	40	theme	fatty	465:469	arg1	acids					471:475	the most abundant fatty acids	447:475	the most abundant fatty acids	447:475	Oleic acid and linoleic acid were the most abundant fatty acids.
25347830	5	41	theme	total	485:489	arg1	acids					497:501	the total amino acids	481:501	the total amino acids in H. parallela	481:517	Of the total amino acids in H. parallela, 47.4% were essential amino acids.
25347830	5	42	from	parallela	509:517	arg1	acids					547:551	essential amino acids	531:551	essential amino acids	531:551	Of the total amino acids in H. parallela, 47.4% were essential amino acids.
25347830	7	43	dep	In	701:702	arg1	vitro					704:708	vitro	704:708	vitro	704:708	In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content.
25347830	1	44	from	source	204:209	arg1	China					214:218	China	214:218	China	214:218	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea) represents a traditional food source in China.
25347830	6	45	theme	acid	564:567	arg1	87					581:582	87	581:582	87	581:582	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	6	45	theme	acid	564:567	arg1	scores					569:574	The amino acid scores	554:574	The amino acid scores	554:574	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	2	46	from	high	274:277	arg1	protein					282:288	protein	282:288	protein (70%)	282:294	Based on nutritional analyses, adult H. parallela is high in protein (70%) and minerals and low in fat.
25347830	2	46	from	high	274:277	arg1	%					293:293	70%	291:293	70%	291:293	Based on nutritional analyses, adult H. parallela is high in protein (70%) and minerals and low in fat.
25347830	2	46	from	high	274:277	arg1	minerals					300:307	minerals	300:307	minerals	300:307	Based on nutritional analyses, adult H. parallela is high in protein (70%) and minerals and low in fat.
25347830	3	47	contain	contained	338:346	arg2	chitin					366:371	approximately 10% chitin	348:371	approximately 10% chitin	348:371	H. parallela contained approximately 10% chitin; the corrected protein content was 66%.
25347830	3	47	contain	contained	338:346	arg1	H.					325:326	H.	325:326	H.	325:326	H. parallela contained approximately 10% chitin; the corrected protein content was 66%.
25347830	5	48	from	acids	547:551	arg1	parallela					509:517	parallela	509:517	parallela	509:517	Of the total amino acids in H. parallela, 47.4% were essential amino acids.
25347830	5	49	theme	acids	497:501	arg1	acids					547:551	essential amino acids	531:551	essential amino acids	531:551	Of the total amino acids in H. parallela, 47.4% were essential amino acids.
25347830	1	50	theme	edible	98:103	arg1	beetle					105:110	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea)	88:172	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea)	88:172	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea) represents a traditional food source in China.
25347830	3	51	dep	H.	325:326	arg1	parallela					328:336	H. parallela	325:336	H. parallela	325:336	H. parallela contained approximately 10% chitin; the corrected protein content was 66%.
25347830	2	52	theme	H.	258:259	arg1	parallela					261:269	adult H. parallela	252:269	adult H. parallela	252:269	Based on nutritional analyses, adult H. parallela is high in protein (70%) and minerals and low in fat.
25347830	6	53	theme	protein	630:636	arg1	contents					638:645	the corrected crude and net protein contents	602:645	the corrected crude and net protein contents	602:645	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	2	54	from	protein	282:288	arg1	high					274:277	high	274:277	high	274:277	Based on nutritional analyses, adult H. parallela is high in protein (70%) and minerals and low in fat.
25347830	0	55	theme	Holotrichia	65:75	arg1	parallela					77:85	the edible beetle Holotrichia parallela	47:85	the edible beetle Holotrichia parallela	47:85	Nutritional composition and protein quality of the edible beetle Holotrichia parallela.
25347830	7	56	theme	acid	787:790	arg1	score					792:796	the protein digestibility-corrected amino acid score	745:796	the protein digestibility-corrected amino acid score	745:796	In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content.
25347830	2	57	theme	adult	252:256	arg1	parallela					261:269	adult H. parallela	252:269	adult H. parallela	252:269	Based on nutritional analyses, adult H. parallela is high in protein (70%) and minerals and low in fat.
25347830	6	58	theme	net	626:628	arg1	contents					638:645	the corrected crude and net protein contents	602:645	the corrected crude and net protein contents	602:645	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	6	59	theme	amino	558:562	arg1	87					581:582	87	581:582	87	581:582	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	6	59	theme	amino	558:562	arg1	scores					569:574	The amino acid scores	554:574	The amino acid scores	554:574	The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid.
25347830	0	60	theme	beetle	58:63	arg1	parallela					77:85	the edible beetle Holotrichia parallela	47:85	the edible beetle Holotrichia parallela	47:85	Nutritional composition and protein quality of the edible beetle Holotrichia parallela.
25347830	8	61	theme	Adult	839:843	arg1	source					877:882	a potential source	865:882	a potential source of proteins and minerals for humans and animals	865:930	Adult H. parallela may be a potential source of proteins and minerals for humans and animals.
25347830	8	61	theme	Adult	839:843	arg1	parallela					848:856	Adult H. parallela	839:856	Adult H. parallela	839:856	Adult H. parallela may be a potential source of proteins and minerals for humans and animals.
25347830	1	62	dep	beetle	105:110	arg1	Motschulsky					134:144	Motschulsky	134:144	Motschulsky	134:144	The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea) represents a traditional food source in China.
25347830	7	63	theme	digestibility-corrected	757:779	arg1	acid					787:790	the protein digestibility-corrected amino acid	745:790	the protein digestibility-corrected amino acid score	745:796	In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content.
24438908	6	0	theme	factor-loaded	1114:1126	arg1	particles					1128:1136	the growth factor-loaded particles	1103:1136	the growth factor-loaded particles alone	1103:1142	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	10	1	theme	encapsulated	1945:1956	arg1	factors					1965:1971	hydrogel encapsulated growth factors	1936:1971	hydrogel encapsulated growth factors alone	1936:1977	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	10	2	theme	pronounced	1805:1814	arg1	replacement					1816:1826	more pronounced replacement	1800:1826	more pronounced replacement by new bone and induced reunion of the bone marrow cavity	1800:1884	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	1	3	theme	side	277:280	arg1	effects					282:288	potential side effects	267:288	potential side effects	267:288	Although rhBMP-2 has excellent ability to accelerate the repair of normal bone defects, limitations of its application exist in the high cost and potential side effects.
24438908	5	4	dep	in	812:813	arg1	vitro					815:819	vitro	815:819	vitro	815:819	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	11	5	dep	promising	2056:2064	arg1	substitute					2066:2075	substitute	2066:2075	substitute for bone tissue engineering	2066:2103	These data provided strong evidence that the composite PH/rhBMP-2/NPs are a promising substitute for bone tissue engineering.
24438908	11	5	dep	promising	2056:2064	arg1	PH/rhBMP-2/NPs					2035:2048	the composite PH/rhBMP-2/NPs	2021:2048	the composite PH/rhBMP-2/NPs	2021:2048	These data provided strong evidence that the composite PH/rhBMP-2/NPs are a promising substitute for bone tissue engineering.
24438908	11	5	dep	promising	2056:2064	arg1	promising					2056:2064	promising	2056:2064	promising	2056:2064	These data provided strong evidence that the composite PH/rhBMP-2/NPs are a promising substitute for bone tissue engineering.
24438908	1	6	theme	bone	195:198	arg1	defects					200:206	normal bone defects	188:206	normal bone defects	188:206	Although rhBMP-2 has excellent ability to accelerate the repair of normal bone defects, limitations of its application exist in the high cost and potential side effects.
24438908	6	7	theme	release	984:990	arg1	kinetics					992:999	In vitro release kinetics	975:999	In vitro release kinetics of rhBMP-2	975:1010	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	9	8	from	effects	1491:1497	arg1	defect					1555:1560	a rabbit radius critical defect	1530:1560	a rabbit radius critical defect	1530:1560	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	5	9	theme	cell	960:963	arg1	ingrowth					965:972	time dependent cell ingrowth	945:972	time dependent cell ingrowth	945:972	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	3	10	theme	chitosan	579:586	arg1	rhBMP-2/NPs					603:613	rhBMP-2/NPs	603:613	rhBMP-2/NPs	603:613	Firstly rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (rhBMP-2/NPs) were prepared and characterized by DLS and TEM.
24438908	3	10	theme	chitosan	579:586	arg1	nanoparticles					588:600	6-O-sulfated chitosan nanoparticles	566:600	6-O-sulfated chitosan nanoparticles (rhBMP-2/NPs)	566:614	Firstly rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (rhBMP-2/NPs) were prepared and characterized by DLS and TEM.
24438908	6	11	theme	In	975:976	arg1	kinetics					992:999	In vitro release kinetics	975:999	In vitro release kinetics of rhBMP-2	975:1010	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	10	12	contain	containing	1726:1735	arg1	hydrogel					1717:1724	the composite hydrogel	1703:1724	the composite hydrogel containing rhBMP-2-loaded nanoparticles	1703:1764	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	10	12	contain	containing	1726:1735	arg2	nanoparticles					1752:1764	rhBMP-2-loaded nanoparticles	1737:1764	rhBMP-2-loaded nanoparticles	1737:1764	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	4	13	theme	Composite	664:672	arg1	PH/rhBMP-2/NPs					685:698	PH/rhBMP-2/NPs	685:698	PH/rhBMP-2/NPs	685:698	Composite materials, PH/rhBMP-2/NPs were developed and investigated by SEM-EDS as well as a series of physical characterizations.
24438908	4	13	theme	Composite	664:672	arg1	materials					674:682	Composite materials	664:682	Composite materials	664:682	Composite materials, PH/rhBMP-2/NPs were developed and investigated by SEM-EDS as well as a series of physical characterizations.
24438908	1	14	theme	application	228:238	arg1	limitations					209:219	limitations	209:219	limitations of its application	209:238	Although rhBMP-2 has excellent ability to accelerate the repair of normal bone defects, limitations of its application exist in the high cost and potential side effects.
24438908	10	15	theme	hydrogel	1936:1943	arg1	factors					1965:1971	hydrogel encapsulated growth factors	1936:1971	hydrogel encapsulated growth factors alone	1936:1977	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	10	16	theme	induced	1844:1850	arg1	reunion					1852:1858	induced reunion	1844:1858	induced reunion	1844:1858	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	1	17	theme	excellent	142:150	arg1	ability					152:158	excellent ability	142:158	excellent ability to accelerate the repair of normal bone defects	142:206	Although rhBMP-2 has excellent ability to accelerate the repair of normal bone defects, limitations of its application exist in the high cost and potential side effects.
24438908	6	18	dep	In	975:976	arg1	vitro					978:982	vitro	978:982	vitro	978:982	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	2	19	dep	incorporating	359:371	arg1	substitute					458:467	substitute	458:467	substitute to realize segmental bone defect repair at a low growth factor dose	458:535	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	10	20	theme	cavity	1879:1884	arg1	replacement					1816:1826	more pronounced replacement	1800:1826	more pronounced replacement by new bone and induced reunion of the bone marrow cavity	1800:1884	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	9	21	theme	rhBMP-2-loaded	1502:1515	arg1	scaffolds					1517:1525	rhBMP-2-loaded scaffolds	1502:1525	rhBMP-2-loaded scaffolds	1502:1525	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	6	22	theme	initial	1041:1047	arg1	burst					1049:1053	a significantly lower initial burst	1019:1053	a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel	1019:1187	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	2	23	theme	growth	518:523	arg1	factor					525:530	a low growth factor	512:530	a low growth factor dose	512:535	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	7	24	theme	rhBMP-2	1256:1262	arg1	bioactivity					1232:1242	The bioactivity	1228:1242	The bioactivity of released rhBMP-2	1228:1262	The bioactivity of released rhBMP-2 was validated by alkaline phosphatase (ALP) activity as well as a mineralization assay.
24438908	10	25	theme	bone	1867:1870	arg1	marrow					1872:1877	the bone marrow	1863:1877	the bone marrow cavity	1863:1884	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	5	26	theme	cell	911:914	arg1	viability					916:924	good cell viability	906:924	good cell viability	906:924	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	9	27	theme	rabbit	1532:1537	arg1	defect					1555:1560	a rabbit radius critical defect	1530:1560	a rabbit radius critical defect	1530:1560	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	8	28	theme	in	1355:1356	arg1	studies					1363:1369	in vivo studies	1355:1369	in vivo studies	1355:1369	In in vivo studies, the PH/rhBMP-2/NPs induced ectopic bone formation in the mouse thigh.
24438908	0	29	dep	2-N	79:81	arg1	6-O-sulfated					84:95	6-O-sulfated	84:95	6-O-sulfated	84:95	Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles.
24438908	9	30	theme	critical	1546:1553	arg1	defect					1555:1560	a rabbit radius critical defect	1530:1560	a rabbit radius critical defect	1530:1560	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	2	31	theme	chitosan	406:413	arg1	nanoparticles					415:427	loaded 2-N, 6-O-sulfated chitosan nanoparticles	381:427	loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone	381:456	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	2	31	theme	chitosan	406:413	arg1	PH/rhBMP-2/NPs					430:443	PH/rhBMP-2/NPs	430:443	PH/rhBMP-2/NPs	430:443	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	0	32	theme	Bone	0:3	arg1	regeneration					5:16	Bone regeneration	0:16	Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2	0:70	Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles.
24438908	9	33	theme	dimensional	1571:1581	arg1	imaging					1616:1622	three dimensional micro-computed tomographic (μCT) imaging	1565:1622	three dimensional micro-computed tomographic (μCT) imaging	1565:1622	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	7	34	theme	mineralization	1330:1343	arg1	assay					1345:1349	a mineralization assay	1328:1349	alkaline phosphatase (ALP) activity as well as a mineralization assay	1281:1349	The bioactivity of released rhBMP-2 was validated by alkaline phosphatase (ALP) activity as well as a mineralization assay.
24438908	11	35	theme	strong	2000:2005	arg1	evidence					2007:2014	strong evidence that the composite PH/rhBMP-2/NPs are a promising substitute for bone tissue engineering	2000:2103	strong evidence that the composite PH/rhBMP-2/NPs are a promising substitute for bone tissue engineering	2000:2103	These data provided strong evidence that the composite PH/rhBMP-2/NPs are a promising substitute for bone tissue engineering.
24438908	4	36	theme	physical	766:773	arg1	characterizations					775:791	physical characterizations	766:791	physical characterizations	766:791	Composite materials, PH/rhBMP-2/NPs were developed and investigated by SEM-EDS as well as a series of physical characterizations.
24438908	5	37	theme	composite	833:841	arg1	hydrogels					862:870	composite photopolymerisable hydrogels	833:870	composite photopolymerisable hydrogels incorporating NPs (PH/NPs)	833:897	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	9	38	theme	tomographic	1598:1608	arg1	imaging					1616:1622	three dimensional micro-computed tomographic (μCT) imaging	1565:1622	three dimensional micro-computed tomographic (μCT) imaging	1565:1622	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	5	39	theme	good	906:909	arg1	viability					916:924	good cell viability	906:924	good cell viability	906:924	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	8	40	theme	bone	1407:1410	arg1	formation					1412:1420	ectopic bone formation	1399:1420	ectopic bone formation	1399:1420	In in vivo studies, the PH/rhBMP-2/NPs induced ectopic bone formation in the mouse thigh.
24438908	0	41	theme	hydrogel	41:48	arg1	rhBMP-2					64:70	photocrosslinked hydrogel incorporating rhBMP-2	24:70	photocrosslinked hydrogel incorporating rhBMP-2	24:70	Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles.
24438908	9	42	theme	μCT	1611:1613	arg1	imaging					1616:1622	three dimensional micro-computed tomographic (μCT) imaging	1565:1622	three dimensional micro-computed tomographic (μCT) imaging	1565:1622	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	2	43	theme	bone	490:493	arg1	repair					502:507	segmental bone defect repair	480:507	segmental bone defect repair	480:507	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	0	44	theme	2-N	79:81	arg1	nanoparticles					106:118	2-N, 6-O-sulfated chitosan nanoparticles	79:118	2-N, 6-O-sulfated chitosan nanoparticles	79:118	Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles.
24438908	10	45	theme	rhBMP-2-loaded	1737:1750	arg1	nanoparticles					1752:1764	rhBMP-2-loaded nanoparticles	1737:1764	rhBMP-2-loaded nanoparticles	1737:1764	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	11	46	theme	composite	2025:2033	arg1	substitute					2066:2075	substitute	2066:2075	substitute for bone tissue engineering	2066:2103	These data provided strong evidence that the composite PH/rhBMP-2/NPs are a promising substitute for bone tissue engineering.
24438908	11	46	theme	composite	2025:2033	arg1	PH/rhBMP-2/NPs					2035:2048	the composite PH/rhBMP-2/NPs	2021:2048	the composite PH/rhBMP-2/NPs	2021:2048	These data provided strong evidence that the composite PH/rhBMP-2/NPs are a promising substitute for bone tissue engineering.
24438908	11	46	theme	composite	2025:2033	arg1	promising					2056:2064	promising	2056:2064	promising	2056:2064	These data provided strong evidence that the composite PH/rhBMP-2/NPs are a promising substitute for bone tissue engineering.
24438908	8	47	theme	mouse	1429:1433	arg1	thigh					1435:1439	the mouse thigh	1425:1439	the mouse thigh	1425:1439	In in vivo studies, the PH/rhBMP-2/NPs induced ectopic bone formation in the mouse thigh.
24438908	2	48	theme	photopolymerisable	331:348	arg1	hydrogel					350:357	a composite photopolymerisable hydrogel	319:357	a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose	319:535	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	10	49	with	resorption	1784:1793	arg1	replacement					1816:1826	more pronounced replacement	1800:1826	more pronounced replacement by new bone and induced reunion of the bone marrow cavity	1800:1884	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	9	50	theme	histological	1625:1636	arg1	analysis					1638:1645	histological analysis	1625:1645	histological analysis	1625:1645	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	7	51	theme	alkaline	1281:1288	arg1	ALP					1303:1305	ALP	1303:1305	ALP	1303:1305	The bioactivity of released rhBMP-2 was validated by alkaline phosphatase (ALP) activity as well as a mineralization assay.
24438908	7	51	theme	alkaline	1281:1288	arg1	phosphatase					1290:1300	alkaline phosphatase	1281:1300	alkaline phosphatase (ALP) activity as well as a mineralization assay	1281:1349	The bioactivity of released rhBMP-2 was validated by alkaline phosphatase (ALP) activity as well as a mineralization assay.
24438908	10	52	theme	growth	1958:1963	arg1	factors					1965:1971	hydrogel encapsulated growth factors	1936:1971	hydrogel encapsulated growth factors alone	1936:1977	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	2	53	theme	loaded	381:386	arg1	nanoparticles					415:427	loaded 2-N, 6-O-sulfated chitosan nanoparticles	381:427	loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone	381:456	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	2	53	theme	loaded	381:386	arg1	PH/rhBMP-2/NPs					430:443	PH/rhBMP-2/NPs	430:443	PH/rhBMP-2/NPs	430:443	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	9	54	theme	biomechanical	1652:1664	arg1	measurements					1666:1677	biomechanical measurements	1652:1677	biomechanical measurements	1652:1677	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	0	55	link	photocrosslinked	24:39	arg1	rhBMP-2					64:70	photocrosslinked hydrogel incorporating rhBMP-2	24:70	photocrosslinked hydrogel incorporating rhBMP-2	24:70	Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles.
24438908	6	56	theme	growth	1107:1112	arg1	particles					1128:1136	the growth factor-loaded particles	1103:1136	the growth factor-loaded particles alone	1103:1142	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	1	57	contain	has	138:140	arg2	ability					152:158	excellent ability	142:158	excellent ability to accelerate the repair of normal bone defects	142:206	Although rhBMP-2 has excellent ability to accelerate the repair of normal bone defects, limitations of its application exist in the high cost and potential side effects.
24438908	1	57	contain	has	138:140	arg1	rhBMP-2					130:136	rhBMP-2	130:136	rhBMP-2	130:136	Although rhBMP-2 has excellent ability to accelerate the repair of normal bone defects, limitations of its application exist in the high cost and potential side effects.
24438908	6	58	from	system	1082:1087	arg1	release					1055:1061	a significantly lower initial burst release	1019:1061	a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel	1019:1187	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	1	59	theme	potential	267:275	arg1	effects					282:288	potential side effects	267:288	potential side effects	267:288	Although rhBMP-2 has excellent ability to accelerate the repair of normal bone defects, limitations of its application exist in the high cost and potential side effects.
24438908	6	60	theme	slow	1204:1207	arg1	release					1209:1215	a slow release	1202:1215	a slow release over time	1202:1225	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	1	61	theme	normal	188:193	arg1	defects					200:206	normal bone defects	188:206	normal bone defects	188:206	Although rhBMP-2 has excellent ability to accelerate the repair of normal bone defects, limitations of its application exist in the high cost and potential side effects.
24438908	1	62	theme	defects	200:206	arg1	repair					178:183	the repair	174:183	the repair of normal bone defects	174:206	Although rhBMP-2 has excellent ability to accelerate the repair of normal bone defects, limitations of its application exist in the high cost and potential side effects.
24438908	3	63	theme	6-O-sulfated	566:577	arg1	rhBMP-2/NPs					603:613	rhBMP-2/NPs	603:613	rhBMP-2/NPs	603:613	Firstly rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (rhBMP-2/NPs) were prepared and characterized by DLS and TEM.
24438908	3	63	theme	6-O-sulfated	566:577	arg1	nanoparticles					588:600	6-O-sulfated chitosan nanoparticles	566:600	6-O-sulfated chitosan nanoparticles (rhBMP-2/NPs)	566:614	Firstly rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (rhBMP-2/NPs) were prepared and characterized by DLS and TEM.
24438908	5	64	theme	photopolymerisable	843:860	arg1	hydrogels					862:870	composite photopolymerisable hydrogels	833:870	composite photopolymerisable hydrogels incorporating NPs (PH/NPs)	833:897	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	8	65	dep	in	1355:1356	arg1	vivo					1358:1361	vivo	1358:1361	vivo	1358:1361	In in vivo studies, the PH/rhBMP-2/NPs induced ectopic bone formation in the mouse thigh.
24438908	5	66	theme	cell	927:930	arg1	adhesion					932:939	cell adhesion	927:939	cell adhesion	927:939	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	10	67	theme	gradual	1776:1782	arg1	resorption					1784:1793	gradual resorption	1776:1793	gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity	1776:1884	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	10	68	theme	new	1831:1833	arg1	bone					1835:1838	new bone	1831:1838	new bone	1831:1838	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	6	69	theme	burst	1049:1053	arg1	release					1055:1061	a significantly lower initial burst release	1019:1061	a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel	1019:1187	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	7	70	theme	released	1247:1254	arg1	rhBMP-2					1256:1262	released rhBMP-2	1247:1262	released rhBMP-2	1247:1262	The bioactivity of released rhBMP-2 was validated by alkaline phosphatase (ALP) activity as well as a mineralization assay.
24438908	10	71	theme	marrow	1872:1877	arg1	cavity					1879:1884	the bone marrow cavity	1863:1884	the bone marrow cavity	1863:1884	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	9	72	theme	scaffolds	1517:1525	arg1	effects					1491:1497	the in vivo effects	1479:1497	the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect	1479:1560	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	6	73	theme	lower	1035:1039	arg1	burst					1049:1053	a significantly lower initial burst	1019:1053	a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel	1019:1187	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	2	74	theme	low	514:516	arg1	factor					525:530	a low growth factor	512:530	a low growth factor dose	512:535	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	0	75	theme	chitosan	97:104	arg1	nanoparticles					106:118	2-N, 6-O-sulfated chitosan nanoparticles	79:118	2-N, 6-O-sulfated chitosan nanoparticles	79:118	Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles.
24438908	9	76	theme	radius	1539:1544	arg1	defect					1555:1560	a rabbit radius critical defect	1530:1560	a rabbit radius critical defect	1530:1560	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	11	77	theme	tissue	2086:2091	arg1	engineering					2093:2103	bone tissue engineering	2081:2103	bone tissue engineering	2081:2103	These data provided strong evidence that the composite PH/rhBMP-2/NPs are a promising substitute for bone tissue engineering.
24438908	6	78	theme	rhBMP-2	1004:1010	arg1	kinetics					992:999	In vitro release kinetics	975:999	In vitro release kinetics of rhBMP-2	975:1010	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	4	79	theme	characterizations	775:791	arg1	series					756:761	a series	754:761	SEM-EDS as well as a series of physical characterizations	735:791	Composite materials, PH/rhBMP-2/NPs were developed and investigated by SEM-EDS as well as a series of physical characterizations.
24438908	4	79	theme	characterizations	775:791	arg1	SEM-EDS					735:741	SEM-EDS	735:741	SEM-EDS as well as a series of physical characterizations	735:791	Composite materials, PH/rhBMP-2/NPs were developed and investigated by SEM-EDS as well as a series of physical characterizations.
24438908	10	80	theme	composite	1707:1715	arg1	hydrogel					1717:1724	the composite hydrogel	1703:1724	the composite hydrogel containing rhBMP-2-loaded nanoparticles	1703:1764	Animals implanted with the composite hydrogel containing rhBMP-2-loaded nanoparticles underwent gradual resorption with more pronounced replacement by new bone and induced reunion of the bone marrow cavity at 12 weeks, compared with animals implanted with hydrogel encapsulated growth factors alone.
24438908	2	81	theme	factor	525:530	arg1	dose					532:535	a low growth factor dose	512:535	a low growth factor dose	512:535	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	2	82	dep	2-N	388:390	arg1	6-O-sulfated					393:404	6-O-sulfated	393:404	6-O-sulfated	393:404	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	5	83	theme	cell	821:824	arg1	model					826:830	an in vitro cell model	809:830	an in vitro cell model	809:830	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	5	83	theme	cell	821:824	arg1	hMSCs					800:804	hMSCs	800:804	hMSCs	800:804	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	5	84	theme	dependent	950:958	arg1	ingrowth					965:972	time dependent cell ingrowth	945:972	time dependent cell ingrowth	945:972	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	6	85	dep	system	1082:1087	arg1	compared					1089:1096	compared	1089:1096	compared with the growth factor-loaded particles alone	1089:1142	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	6	85	dep	system	1082:1087	arg1	encapsulated					1147:1158	encapsulated	1147:1158	encapsulated directly within the hydrogel	1147:1187	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	11	86	theme	bone	2081:2084	arg1	engineering					2093:2103	bone tissue engineering	2081:2103	bone tissue engineering	2081:2103	These data provided strong evidence that the composite PH/rhBMP-2/NPs are a promising substitute for bone tissue engineering.
24438908	0	87	theme	photocrosslinked	24:39	arg1	rhBMP-2					64:70	photocrosslinked hydrogel incorporating rhBMP-2	24:70	photocrosslinked hydrogel incorporating rhBMP-2	24:70	Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles.
24438908	9	88	theme	micro-computed	1583:1596	arg1	imaging					1616:1622	three dimensional micro-computed tomographic (μCT) imaging	1565:1622	three dimensional micro-computed tomographic (μCT) imaging	1565:1622	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	6	89	theme	composite	1072:1080	arg1	system					1082:1087	the composite system	1068:1087	the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel	1068:1187	In vitro release kinetics of rhBMP-2 showed a significantly lower initial burst release from the composite system compared with the growth factor-loaded particles alone or encapsulated directly within the hydrogel, followed by a slow release over time.
24438908	0	90	theme	incorporating	50:62	arg1	rhBMP-2					64:70	photocrosslinked hydrogel incorporating rhBMP-2	24:70	photocrosslinked hydrogel incorporating rhBMP-2	24:70	Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles.
24438908	2	91	theme	defect	495:500	arg1	repair					502:507	segmental bone defect repair	480:507	segmental bone defect repair	480:507	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	8	92	theme	ectopic	1399:1405	arg1	formation					1412:1420	ectopic bone formation	1399:1420	ectopic bone formation	1399:1420	In in vivo studies, the PH/rhBMP-2/NPs induced ectopic bone formation in the mouse thigh.
24438908	5	93	theme	in	812:813	arg1	model					826:830	an in vitro cell model	809:830	an in vitro cell model	809:830	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	5	93	theme	in	812:813	arg1	hMSCs					800:804	hMSCs	800:804	hMSCs	800:804	Using hMSCs as an in vitro cell model, composite photopolymerisable hydrogels incorporating NPs (PH/NPs) showed good cell viability, cell adhesion and time dependent cell ingrowth.
24438908	2	94	theme	segmental	480:488	arg1	repair					502:507	segmental bone defect repair	480:507	segmental bone defect repair	480:507	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	7	95	theme	phosphatase	1290:1300	arg1	activity					1308:1315	alkaline phosphatase (ALP) activity	1281:1315	alkaline phosphatase (ALP) activity as well as a mineralization assay	1281:1349	The bioactivity of released rhBMP-2 was validated by alkaline phosphatase (ALP) activity as well as a mineralization assay.
24438908	9	96	dep	in	1483:1484	arg1	vivo					1486:1489	vivo	1486:1489	vivo	1486:1489	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	2	97	theme	composite	321:329	arg1	hydrogel					350:357	a composite photopolymerisable hydrogel	319:357	a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose	319:535	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	9	98	theme	in	1483:1484	arg1	effects					1491:1497	the in vivo effects	1479:1497	the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect	1479:1560	In addition, we further investigated the in vivo effects of rhBMP-2-loaded scaffolds in a rabbit radius critical defect by three dimensional micro-computed tomographic (μCT) imaging, histological analysis, and biomechanical measurements.
24438908	2	99	theme	2-N	388:390	arg1	nanoparticles					415:427	loaded 2-N, 6-O-sulfated chitosan nanoparticles	381:427	loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone	381:456	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	2	99	theme	2-N	388:390	arg1	PH/rhBMP-2/NPs					430:443	PH/rhBMP-2/NPs	430:443	PH/rhBMP-2/NPs	430:443	This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low growth factor dose.
24438908	1	100	theme	high	253:256	arg1	cost					258:261	the high cost	249:261	the high cost	249:261	Although rhBMP-2 has excellent ability to accelerate the repair of normal bone defects, limitations of its application exist in the high cost and potential side effects.
25467440	6	0	theme	profound	1143:1150	arg1	changes					1152:1158	profound changes	1143:1158	profound changes in macrophage actin dynamics	1143:1187	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	0	theme	profound	1143:1150	arg1	markers					1011:1017	phagosome maturation markers	990:1017	phagosome maturation markers	990:1017	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	2	1	theme	fungal	370:375	arg1	cells					377:381	fungal cells	370:381	fungal cells	370:381	Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells.
25467440	6	2	theme	O-mannan	938:945	arg1	Loss					920:923	Loss	920:923	Loss of cell wall O-mannan	920:945	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	9	3	theme	phagosomes	1719:1728	arg1	maturation					1705:1714	the maturation	1701:1714	the maturation of phagosomes	1701:1728	Yet comparatively little is known about what controls the maturation of phagosomes following ingestion of fungal cells.
25467440	10	4	theme	filamentous	1882:1892	arg1	growth					1894:1899	filamentous growth	1882:1899	filamentous growth	1882:1899	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	6	5	theme	cells	1233:1237	arg1	changes					1152:1158	profound changes	1143:1158	profound changes in macrophage actin dynamics	1143:1187	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	5	theme	cells	1233:1237	arg1	markers					1011:1017	phagosome maturation markers	990:1017	phagosome maturation markers	990:1017	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	5	theme	cells	1233:1237	arg1	changes					1029:1035	distinct changes	1020:1035	distinct changes in Rab GTPase acquisition by the maturing phagosome	1020:1087	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	5	theme	cells	1233:1237	arg1	growth					1106:1111	impaired hyphal growth	1090:1111	impaired hyphal growth within macrophage phagosomes	1090:1140	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	5	theme	cells	1233:1237	arg1	ability					1215:1221	a reduced ability	1205:1221	a reduced ability of fungal cells to escape from macrophage phagosomes	1205:1274	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	2	6	theme	phagocytes	309:318	arg1	capacity					284:291	the capacity	280:291	the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells	280:381	Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells.
25467440	6	7	theme	cell	928:931	arg1	O-mannan					938:945	cell wall O-mannan	928:945	cell wall O-mannan	928:945	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	5	8	theme	fungal	901:906	arg1	morphology					908:917	fungal morphology	901:917	fungal morphology	901:917	The ability of C. albicans to delay phagosome maturation is dependent on cell wall composition and fungal morphology.
25467440	9	9	theme	fungal	1753:1758	arg1	cells					1760:1764	fungal cells	1753:1764	fungal cells	1753:1764	Yet comparatively little is known about what controls the maturation of phagosomes following ingestion of fungal cells.
25467440	6	10	theme	reduced	1207:1213	arg1	markers					1011:1017	phagosome maturation markers	990:1017	phagosome maturation markers	990:1017	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	10	theme	reduced	1207:1213	arg1	ability					1215:1221	a reduced ability	1205:1221	a reduced ability of fungal cells to escape from macrophage phagosomes	1205:1274	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	2	11	theme	immune	334:339	arg1	system					341:346	the innate immune system	323:346	the innate immune system	323:346	Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells.
25467440	11	12	theme	fungal	2218:2223	arg1	pathogen					2225:2232	this fungal pathogen	2213:2232	this fungal pathogen	2213:2232	We have demonstrated that cell wall glycosylation and yeast-hypha morphogenesis are required for disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes.
25467440	11	13	theme	cell	2040:2043	arg1	glycosylation					2050:2062	cell wall glycosylation	2040:2062	cell wall glycosylation	2040:2062	We have demonstrated that cell wall glycosylation and yeast-hypha morphogenesis are required for disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes.
25467440	7	14	theme	enhanced	1425:1432	arg1	maturation					1444:1453	enhanced phagosome maturation	1425:1453	enhanced phagosome maturation	1425:1453	The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.
25467440	11	15	theme	processes	2130:2138	arg1	disruption					2111:2120	disruption	2111:2120	disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes	2111:2260	We have demonstrated that cell wall glycosylation and yeast-hypha morphogenesis are required for disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes.
25467440	5	16	theme	cell	875:878	arg1	composition					885:895	cell wall composition	875:895	cell wall composition	875:895	The ability of C. albicans to delay phagosome maturation is dependent on cell wall composition and fungal morphology.
25467440	6	17	theme	maturation	1000:1009	arg1	changes					1152:1158	profound changes	1143:1158	profound changes in macrophage actin dynamics	1143:1187	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	17	theme	maturation	1000:1009	arg1	markers					1011:1017	phagosome maturation markers	990:1017	phagosome maturation markers	990:1017	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	17	theme	maturation	1000:1009	arg1	changes					1029:1035	distinct changes	1020:1035	distinct changes in Rab GTPase acquisition by the maturing phagosome	1020:1087	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	17	theme	maturation	1000:1009	arg1	ability					1215:1221	a reduced ability	1205:1221	a reduced ability of fungal cells to escape from macrophage phagosomes	1205:1274	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	17	theme	maturation	1000:1009	arg1	growth					1106:1111	impaired hyphal growth	1090:1111	impaired hyphal growth within macrophage phagosomes	1090:1140	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	12	18	theme	maturation	2447:2456	arg1	stages					2427:2432	different stages	2417:2432	different stages of phagosome maturation and the survival of the pathogen or host	2417:2497	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	4	19	theme	heat-	677:681	arg1	cells					708:712	viable, but not heat- or UV-killed C. albicans cells	661:712	viable, but not heat- or UV-killed C. albicans cells	661:712	Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages.
25467440	8	20	theme	invading	1499:1506	arg1	organisms					1508:1516	invading organisms	1499:1516	invading organisms	1499:1516	IMPORTANCE Innate cells engulf and destroy invading organisms by phagocytosis, which is essential for the elimination of fungal cells to protect against systemic life-threatening infections.
25467440	10	21	dep	C.	1859:1860	arg1	albicans					1862:1869	albicans	1862:1869	albicans	1862:1869	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	2	22	theme	systemic	235:242	arg1	infection					252:260	systemic Candida infection	235:260	systemic Candida infection	235:260	Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells.
25467440	6	23	theme	enhanced	966:973	arg1	acquisition					975:985	enhanced acquisition	966:985	enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes	966:1274	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	12	24	theme	different	2417:2425	arg1	stages					2427:2432	different stages	2417:2432	different stages of phagosome maturation and the survival of the pathogen or host	2417:2497	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	6	25	theme	macrophage	1254:1263	arg1	phagosomes					1265:1274	macrophage phagosomes	1254:1274	macrophage phagosomes	1254:1274	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	4	26	theme	cell	766:769	arg1	lines					771:775	macrophage cell lines	755:775	macrophage cell lines	755:775	Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages.
25467440	10	27	theme	wall	1911:1914	arg1	composition					1916:1926	cell wall composition	1906:1926	cell wall composition	1906:1926	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	1	28	theme	major	138:142	arg1	albicans					124:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	1	28	theme	major	138:142	arg1	pathogen					174:181	a major life-threatening human fungal pathogen	136:181	a major life-threatening human fungal pathogen in the immunocompromised host	136:211	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	11	29	theme	yeast-hypha	2068:2078	arg1	morphogenesis					2080:2092	yeast-hypha morphogenesis	2068:2092	yeast-hypha morphogenesis	2068:2092	We have demonstrated that cell wall glycosylation and yeast-hypha morphogenesis are required for disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes.
25467440	1	30	theme	human	161:165	arg1	albicans					124:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	1	30	theme	human	161:165	arg1	pathogen					174:181	a major life-threatening human fungal pathogen	136:181	a major life-threatening human fungal pathogen in the immunocompromised host	136:211	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	8	31	theme	systemic	1609:1616	arg1	infections					1635:1644	systemic life-threatening infections	1609:1644	systemic life-threatening infections	1609:1644	IMPORTANCE Innate cells engulf and destroy invading organisms by phagocytosis, which is essential for the elimination of fungal cells to protect against systemic life-threatening infections.
25467440	4	32	theme	phagosome	731:739	arg1	maturation					741:750	phagosome maturation	731:750	phagosome maturation	731:750	Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages.
25467440	12	33	with	interactions	2313:2324	arg1	cells					2361:2365	phagocytic cells	2350:2365	phagocytic cells	2350:2365	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	0	34	theme	macrophage	72:81	arg1	maturation					93:102	macrophage phagosome maturation	72:102	macrophage phagosome maturation	72:102	Candida albicans hypha formation and mannan masking of β-glucan inhibit macrophage phagosome maturation.
25467440	6	35	theme	Rab	1040:1042	arg1	acquisition					1051:1061	Rab GTPase acquisition	1040:1061	Rab GTPase acquisition by the maturing phagosome	1040:1087	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	12	36	theme	pathogens	2335:2343	arg1	interactions					2313:2324	interactions	2313:2324	interactions of other pathogens with phagocytic cells	2313:2365	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	7	37	theme	inner	1345:1349	arg1	wall					1356:1359	the inner cell wall	1341:1359	the inner cell wall	1341:1359	The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.
25467440	12	38	theme	phagocytic	2350:2359	arg1	cells					2361:2365	phagocytic cells	2350:2365	phagocytic cells	2350:2365	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	6	39	from	ability	1215:1221	arg1	dynamics					1180:1187	macrophage actin dynamics	1163:1187	macrophage actin dynamics	1163:1187	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	39	from	ability	1215:1221	arg1	acquisition					1051:1061	Rab GTPase acquisition	1040:1061	Rab GTPase acquisition by the maturing phagosome	1040:1087	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	12	40	theme	pathogen	2482:2489	arg1	maturation					2447:2456	phagosome maturation	2437:2456	phagosome maturation	2437:2456	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	12	40	theme	pathogen	2482:2489	arg1	survival					2466:2473	the survival	2462:2473	the survival of the pathogen or host	2462:2497	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	6	41	theme	macrophage	1120:1129	arg1	phagosomes					1131:1140	macrophage phagosomes	1120:1140	macrophage phagosomes	1120:1140	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	3	42	theme	pathogens	396:404	arg1	number					386:391	A number	384:391	A number of pathogens, including C. albicans,	384:428	A number of pathogens, including C. albicans, have evolved mechanisms that attenuate the efficiency of phagosome-mediated inactivation, promoting their survival and replication within the host.
25467440	3	42	theme	pathogens	396:404	arg1	albicans					420:427	C. albicans	417:427	C. albicans	417:427	A number of pathogens, including C. albicans, have evolved mechanisms that attenuate the efficiency of phagosome-mediated inactivation, promoting their survival and replication within the host.
25467440	8	43	theme	fungal	1577:1582	arg1	cells					1584:1588	fungal cells	1577:1588	fungal cells	1577:1588	IMPORTANCE Innate cells engulf and destroy invading organisms by phagocytosis, which is essential for the elimination of fungal cells to protect against systemic life-threatening infections.
25467440	0	44	theme	Candida	0:6	arg1	mannan					37:42	mannan	37:42	mannan	37:42	Candida albicans hypha formation and mannan masking of β-glucan inhibit macrophage phagosome maturation.
25467440	0	44	theme	Candida	0:6	arg1	formation					23:31	hypha formation	17:31	hypha formation	17:31	Candida albicans hypha formation and mannan masking of β-glucan inhibit macrophage phagosome maturation.
25467440	0	44	theme	Candida	0:6	arg1	albicans					8:15	Candida albicans	0:15	Candida albicans hypha formation and mannan masking of β-glucan	0:62	Candida albicans hypha formation and mannan masking of β-glucan inhibit macrophage phagosome maturation.
25467440	0	45	theme	hypha	17:21	arg1	formation					23:31	hypha formation	17:31	hypha formation	17:31	Candida albicans hypha formation and mannan masking of β-glucan inhibit macrophage phagosome maturation.
25467440	0	45	theme	hypha	17:21	arg1	albicans					8:15	Candida albicans	0:15	Candida albicans hypha formation and mannan masking of β-glucan	0:62	Candida albicans hypha formation and mannan masking of β-glucan inhibit macrophage phagosome maturation.
25467440	1	46	theme	Candida	116:122	arg1	albicans					124:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	1	46	theme	Candida	116:122	arg1	pathogen					174:181	a major life-threatening human fungal pathogen	136:181	a major life-threatening human fungal pathogen in the immunocompromised host	136:211	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	6	47	theme	impaired	1090:1097	arg1	markers					1011:1017	phagosome maturation markers	990:1017	phagosome maturation markers	990:1017	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	47	theme	impaired	1090:1097	arg1	growth					1106:1111	impaired hyphal growth	1090:1111	impaired hyphal growth within macrophage phagosomes	1090:1140	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	5	48	theme	albicans	820:827	arg1	ability					806:812	The ability	802:812	The ability of C. albicans to delay phagosome maturation	802:857	The ability of C. albicans to delay phagosome maturation is dependent on cell wall composition and fungal morphology.
25467440	5	48	theme	albicans	820:827	arg1	dependent					862:870	dependent	862:870	dependent	862:870	The ability of C. albicans to delay phagosome maturation is dependent on cell wall composition and fungal morphology.
25467440	8	49	theme	life-threatening	1618:1633	arg1	infections					1635:1644	systemic life-threatening infections	1609:1644	systemic life-threatening infections	1609:1644	IMPORTANCE Innate cells engulf and destroy invading organisms by phagocytosis, which is essential for the elimination of fungal cells to protect against systemic life-threatening infections.
25467440	10	50	theme	live-cell	1775:1783	arg1	microscopy					1785:1794	live-cell microscopy	1775:1794	live-cell microscopy	1775:1794	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	8	51	theme	Innate	1467:1472	arg1	cells					1474:1478	IMPORTANCE Innate cells	1456:1478	IMPORTANCE Innate cells	1456:1478	IMPORTANCE Innate cells engulf and destroy invading organisms by phagocytosis, which is essential for the elimination of fungal cells to protect against systemic life-threatening infections.
25467440	3	52	theme	phagosome-mediated	487:504	arg1	inactivation					506:517	phagosome-mediated inactivation	487:517	phagosome-mediated inactivation	487:517	A number of pathogens, including C. albicans, have evolved mechanisms that attenuate the efficiency of phagosome-mediated inactivation, promoting their survival and replication within the host.
25467440	4	53	dep	C.	696:697	arg1	albicans					699:706	albicans	699:706	albicans	699:706	Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages.
25467440	6	54	theme	macrophage	1163:1172	arg1	dynamics					1180:1187	macrophage actin dynamics	1163:1187	macrophage actin dynamics	1163:1187	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	12	55	theme	host	2494:2497	arg1	maturation					2447:2456	phagosome maturation	2437:2456	phagosome maturation	2437:2456	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	12	55	theme	host	2494:2497	arg1	survival					2466:2473	the survival	2462:2473	the survival of the pathogen or host	2462:2497	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	10	56	theme	protein	1812:1818	arg1	reporter					1820:1827	fluorescent protein reporter	1800:1827	fluorescent protein reporter	1800:1827	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	7	57	theme	O-mannan	1299:1306	arg1	loss					1281:1284	The loss	1277:1284	The loss of cell wall O-mannan	1277:1306	The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.
25467440	10	58	theme	host	1996:1999	arg1	macrophages					2001:2011	host macrophages	1996:2011	host macrophages	1996:2011	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	6	59	from	changes	1152:1158	arg1	dynamics					1180:1187	macrophage actin dynamics	1163:1187	macrophage actin dynamics	1163:1187	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	59	from	changes	1152:1158	arg1	acquisition					1051:1061	Rab GTPase acquisition	1040:1061	Rab GTPase acquisition by the maturing phagosome	1040:1087	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	12	60	theme	specific	2382:2389	arg1	features					2401:2408	specific microbial features	2382:2408	specific microbial features	2382:2408	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	11	61	theme	pathogen	2225:2232	arg1	escape					2203:2208	escape	2203:2208	escape	2203:2208	We have demonstrated that cell wall glycosylation and yeast-hypha morphogenesis are required for disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes.
25467440	11	61	theme	pathogen	2225:2232	arg1	survival					2190:2197	survival	2190:2197	survival	2190:2197	We have demonstrated that cell wall glycosylation and yeast-hypha morphogenesis are required for disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes.
25467440	7	62	theme	cell	1289:1292	arg1	O-mannan					1299:1306	cell wall O-mannan	1289:1306	cell wall O-mannan	1289:1306	The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.
25467440	10	63	theme	pathogen	1980:1987	arg1	maturation					1945:1954	phagosome maturation	1935:1954	phagosome maturation	1935:1954	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	10	63	theme	pathogen	1980:1987	arg1	survival					1964:1971	the survival	1960:1971	the survival of the pathogen within host macrophages	1960:2011	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	6	64	from	growth	1106:1111	arg1	dynamics					1180:1187	macrophage actin dynamics	1163:1187	macrophage actin dynamics	1163:1187	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	64	from	growth	1106:1111	arg1	acquisition					1051:1061	Rab GTPase acquisition	1040:1061	Rab GTPase acquisition by the maturing phagosome	1040:1087	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	65	theme	wall	933:936	arg1	O-mannan					938:945	cell wall O-mannan	928:945	cell wall O-mannan	928:945	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	66	theme	fungal	1226:1231	arg1	cells					1233:1237	fungal cells	1226:1237	fungal cells	1226:1237	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	4	67	theme	live-cell	629:637	arg1	imaging					639:645	live-cell imaging	629:645	live-cell imaging	629:645	Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages.
25467440	2	68	theme	professional	296:307	arg1	phagocytes					309:318	professional phagocytes	296:318	professional phagocytes of the innate immune system	296:346	Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells.
25467440	11	69	theme	host	2246:2249	arg1	phagocytes					2251:2260	host phagocytes	2246:2260	host phagocytes	2246:2260	We have demonstrated that cell wall glycosylation and yeast-hypha morphogenesis are required for disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes.
25467440	2	70	theme	system	341:346	arg1	phagocytes					309:318	professional phagocytes	296:318	professional phagocytes of the innate immune system	296:346	Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells.
25467440	1	71	theme	immunocompromised	190:206	arg1	host					208:211	the immunocompromised host	186:211	the immunocompromised host	186:211	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	6	72	theme	distinct	1020:1027	arg1	markers					1011:1017	phagosome maturation markers	990:1017	phagosome maturation markers	990:1017	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	72	theme	distinct	1020:1027	arg1	changes					1029:1035	distinct changes	1020:1035	distinct changes in Rab GTPase acquisition by the maturing phagosome	1020:1087	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	9	73	theme	cells	1760:1764	arg1	ingestion					1740:1748	ingestion	1740:1748	ingestion of fungal cells	1740:1764	Yet comparatively little is known about what controls the maturation of phagosomes following ingestion of fungal cells.
25467440	7	74	from	exposure	1317:1324	arg1	wall					1356:1359	the inner cell wall	1341:1359	the inner cell wall	1341:1359	The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.
25467440	4	75	theme	C.	696:697	arg1	cells					708:712	viable, but not heat- or UV-killed C. albicans cells	661:712	viable, but not heat- or UV-killed C. albicans cells	661:712	Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages.
25467440	2	76	theme	innate	327:332	arg1	system					341:346	the innate immune system	323:346	the innate immune system	323:346	Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells.
25467440	6	77	theme	markers	1011:1017	arg1	acquisition					975:985	enhanced acquisition	966:985	enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes	966:1274	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	7	78	theme	phagosome	1434:1442	arg1	maturation					1444:1453	enhanced phagosome maturation	1425:1453	enhanced phagosome maturation	1425:1453	The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.
25467440	12	79	dep	pathogen	2482:2489	arg1	the					2478:2480	the	2478:2480	the	2478:2480	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	11	80	theme	wall	2045:2048	arg1	glycosylation					2050:2062	cell wall glycosylation	2040:2062	cell wall glycosylation	2040:2062	We have demonstrated that cell wall glycosylation and yeast-hypha morphogenesis are required for disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes.
25467440	5	81	theme	wall	880:883	arg1	composition					885:895	cell wall composition	875:895	cell wall composition	875:895	The ability of C. albicans to delay phagosome maturation is dependent on cell wall composition and fungal morphology.
25467440	6	82	theme	phagosome	990:998	arg1	changes					1152:1158	profound changes	1143:1158	profound changes in macrophage actin dynamics	1143:1187	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	82	theme	phagosome	990:998	arg1	markers					1011:1017	phagosome maturation markers	990:1017	phagosome maturation markers	990:1017	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	82	theme	phagosome	990:998	arg1	changes					1029:1035	distinct changes	1020:1035	distinct changes in Rab GTPase acquisition by the maturing phagosome	1020:1087	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	82	theme	phagosome	990:998	arg1	ability					1215:1221	a reduced ability	1205:1221	a reduced ability of fungal cells to escape from macrophage phagosomes	1205:1274	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	82	theme	phagosome	990:998	arg1	growth					1106:1111	impaired hyphal growth	1090:1111	impaired hyphal growth within macrophage phagosomes	1090:1140	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	12	83	theme	survival	2466:2473	arg1	stages					2427:2432	different stages	2417:2432	different stages of phagosome maturation and the survival of the pathogen or host	2417:2497	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	2	84	theme	Candida	244:250	arg1	infection					252:260	systemic Candida infection	235:260	systemic Candida infection	235:260	Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells.
25467440	10	85	theme	C.	1859:1860	arg1	viability					1871:1879	C. albicans viability	1859:1879	C. albicans viability	1859:1879	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	6	86	theme	maturing	1070:1077	arg1	phagosome					1079:1087	the maturing phagosome	1066:1087	the maturing phagosome	1066:1087	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	12	87	theme	phagosome	2437:2445	arg1	maturation					2447:2456	phagosome maturation	2437:2456	phagosome maturation	2437:2456	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	4	88	theme	macrophage	755:764	arg1	lines					771:775	macrophage cell lines	755:775	macrophage cell lines	755:775	Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages.
25467440	10	89	theme	cell	1906:1909	arg1	composition					1916:1926	cell wall composition	1906:1926	cell wall composition	1906:1926	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	1	90	theme	life-threatening	144:159	arg1	albicans					124:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	1	90	theme	life-threatening	144:159	arg1	pathogen					174:181	a major life-threatening human fungal pathogen	136:181	a major life-threatening human fungal pathogen in the immunocompromised host	136:211	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	12	91	theme	microbial	2391:2399	arg1	features					2401:2408	specific microbial features	2382:2408	specific microbial features	2382:2408	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	0	92	theme	phagosome	83:91	arg1	maturation					93:102	macrophage phagosome maturation	72:102	macrophage phagosome maturation	72:102	Candida albicans hypha formation and mannan masking of β-glucan inhibit macrophage phagosome maturation.
25467440	1	93	theme	fungal	167:172	arg1	albicans					124:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	1	93	theme	fungal	167:172	arg1	pathogen					174:181	a major life-threatening human fungal pathogen	136:181	a major life-threatening human fungal pathogen in the immunocompromised host	136:211	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	6	94	theme	GTPase	1044:1049	arg1	acquisition					1051:1061	Rab GTPase acquisition	1040:1061	Rab GTPase acquisition by the maturing phagosome	1040:1087	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	1	95	from	pathogen	174:181	arg1	host					208:211	the immunocompromised host	186:211	the immunocompromised host	186:211	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	12	96	theme	other	2329:2333	arg1	pathogens					2335:2343	other pathogens	2329:2343	other pathogens	2329:2343	The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.
25467440	10	97	dep	microscopy	1785:1794	arg1	macrophages					1829:1839	macrophages	1829:1839	macrophages	1829:1839	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	8	98	theme	cells	1584:1588	arg1	elimination					1562:1572	the elimination	1558:1572	the elimination of fungal cells	1558:1588	IMPORTANCE Innate cells engulf and destroy invading organisms by phagocytosis, which is essential for the elimination of fungal cells to protect against systemic life-threatening infections.
25467440	6	99	from	changes	1029:1035	arg1	dynamics					1180:1187	macrophage actin dynamics	1163:1187	macrophage actin dynamics	1163:1187	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	99	from	changes	1029:1035	arg1	acquisition					1051:1061	Rab GTPase acquisition	1040:1061	Rab GTPase acquisition by the maturing phagosome	1040:1087	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	5	100	theme	phagosome	838:846	arg1	maturation					848:857	phagosome maturation	838:857	phagosome maturation	838:857	The ability of C. albicans to delay phagosome maturation is dependent on cell wall composition and fungal morphology.
25467440	7	101	theme	cell	1351:1354	arg1	wall					1356:1359	the inner cell wall	1341:1359	the inner cell wall	1341:1359	The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.
25467440	11	102	theme	host	2125:2128	arg1	processes					2130:2138	host processes	2125:2138	host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes	2125:2260	We have demonstrated that cell wall glycosylation and yeast-hypha morphogenesis are required for disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes.
25467440	10	103	used	used	1770:1773	arg2	We					1767:1768	We	1767:1768	We	1767:1768	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	1	104	theme	UNLABELLED	105:114	arg1	albicans					124:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans	105:131	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	1	104	theme	UNLABELLED	105:114	arg1	pathogen					174:181	a major life-threatening human fungal pathogen	136:181	a major life-threatening human fungal pathogen in the immunocompromised host	136:211	UNLABELLED Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host.
25467440	6	105	theme	hyphal	1099:1104	arg1	markers					1011:1017	phagosome maturation markers	990:1017	phagosome maturation markers	990:1017	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	6	105	theme	hyphal	1099:1104	arg1	growth					1106:1111	impaired hyphal growth	1090:1111	impaired hyphal growth within macrophage phagosomes	1090:1140	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	4	106	theme	host-pathogen	596:608	arg1	interactions					610:621	host-pathogen interactions	596:621	host-pathogen interactions using live-cell imaging	596:645	Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages.
25467440	4	107	theme	primary	781:787	arg1	macrophages					789:799	primary macrophages	781:799	primary macrophages	781:799	Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages.
25467440	5	108	theme	C.	817:818	arg1	albicans					820:827	C. albicans	817:827	C. albicans	817:827	The ability of C. albicans to delay phagosome maturation is dependent on cell wall composition and fungal morphology.
25467440	7	109	theme	β-glucan	1329:1336	arg1	exposure					1317:1324	exposure	1317:1324	exposure of β-glucan in the inner cell wall	1317:1359	The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.
25467440	11	110	gly	glycosylation	2050:2062	arg1	processes					2130:2138	host processes	2125:2138	host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes	2125:2260	We have demonstrated that cell wall glycosylation and yeast-hypha morphogenesis are required for disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes.
25467440	10	111	theme	phagosome	1935:1943	arg1	maturation					1945:1954	phagosome maturation	1935:1954	phagosome maturation	1935:1954	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
25467440	3	112	theme	C.	417:418	arg1	albicans					420:427	C. albicans	417:427	C. albicans	417:427	A number of pathogens, including C. albicans, have evolved mechanisms that attenuate the efficiency of phagosome-mediated inactivation, promoting their survival and replication within the host.
25467440	6	113	theme	actin	1174:1178	arg1	dynamics					1180:1187	macrophage actin dynamics	1163:1187	macrophage actin dynamics	1163:1187	Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes.
25467440	2	114	theme	Host	214:217	arg1	defense					219:225	Host defense	214:225	Host defense against systemic Candida infection	214:260	Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells.
25467440	8	115	theme	IMPORTANCE	1456:1465	arg1	cells					1474:1478	IMPORTANCE Innate cells	1456:1478	IMPORTANCE Innate cells	1456:1478	IMPORTANCE Innate cells engulf and destroy invading organisms by phagocytosis, which is essential for the elimination of fungal cells to protect against systemic life-threatening infections.
25467440	3	116	theme	inactivation	506:517	arg1	efficiency					473:482	the efficiency	469:482	the efficiency of phagosome-mediated inactivation	469:517	A number of pathogens, including C. albicans, have evolved mechanisms that attenuate the efficiency of phagosome-mediated inactivation, promoting their survival and replication within the host.
25467440	0	117	dep	albicans	8:15	arg1	mannan					37:42	mannan	37:42	mannan	37:42	Candida albicans hypha formation and mannan masking of β-glucan inhibit macrophage phagosome maturation.
25467440	0	117	dep	albicans	8:15	arg1	formation					23:31	hypha formation	17:31	hypha formation	17:31	Candida albicans hypha formation and mannan masking of β-glucan inhibit macrophage phagosome maturation.
25467440	0	117	dep	albicans	8:15	arg1	albicans					8:15	Candida albicans	0:15	Candida albicans hypha formation and mannan masking of β-glucan	0:62	Candida albicans hypha formation and mannan masking of β-glucan inhibit macrophage phagosome maturation.
25467440	7	118	theme	wall	1294:1297	arg1	O-mannan					1299:1306	cell wall O-mannan	1289:1306	cell wall O-mannan	1289:1306	The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.
25467440	4	119	theme	viable	661:666	arg1	cells					708:712	viable, but not heat- or UV-killed C. albicans cells	661:712	viable, but not heat- or UV-killed C. albicans cells	661:712	Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages.
25467440	10	120	theme	fluorescent	1800:1810	arg1	reporter					1820:1827	fluorescent protein reporter	1800:1827	fluorescent protein reporter	1800:1827	We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages.
28369914	0	0	theme	growth-and-differentiation	40:65	arg1	zone					67:70	the leaf growth-and-differentiation zone	31:70	the leaf growth-and-differentiation zone of grasses	31:81	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	0	1	from	O	8:8	arg1	zone					67:70	the leaf growth-and-differentiation zone	31:70	the leaf growth-and-differentiation zone of grasses	31:81	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	5	2	theme	water	1159:1163	arg1	composition					1144:1154	The isotopic composition	1131:1154	The isotopic composition of water in the LGDZ	1131:1175	The isotopic composition of water in the LGDZ was close to that of source water, independent of VPD and much less enriched than previously supposed, but similar to reported xylem water in trees.
28369914	5	2	theme	water	1159:1163	arg1	that					1190:1193	that	1190:1193	that	1190:1193	The isotopic composition of water in the LGDZ was close to that of source water, independent of VPD and much less enriched than previously supposed, but similar to reported xylem water in trees.
28369914	4	3	theme	px	1099:1100	arg1	-values					1102:1108	(apparent) px -values > 1.0	1088:1114	(apparent) px -values > 1.0 in some cases	1088:1128	Deuterium discrimination caused depletion of 2 H in LGDZ water, increasing (apparent) px -values > 1.0 in some cases.
28369914	0	4	theme	leaf	35:38	arg1	zone					67:70	the leaf growth-and-differentiation zone	31:70	the leaf growth-and-differentiation zone of grasses	31:81	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	0	5	theme	dry	126:128	arg1	atmospheres					130:140	both humid and dry atmospheres	111:140	both humid and dry atmospheres	111:140	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	3	6	theme	water	802:806	arg1	proportion					777:786	The proportion	773:786	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD	773:884	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	4	7	theme	LGDZ	1065:1068	arg1	water					1070:1074	LGDZ water	1065:1074	LGDZ water	1065:1074	Deuterium discrimination caused depletion of 2 H in LGDZ water, increasing (apparent) px -values > 1.0 in some cases.
28369914	1	8	dep	important	366:374	arg1	environmental					401:413	environmental	401:413	environmental	401:413	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	9	theme	leaf	203:206	arg1	LGDZ					241:244	LGDZ	241:244	LGDZ	241:244	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	9	theme	leaf	203:206	arg1	zone					235:238	the leaf growth-and-differentiation zone	199:238	the leaf growth-and-differentiation zone	199:238	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	3	10	dep	1.2	869:871	arg1	to					866:867	to	866:867	to	866:867	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	1	11	theme	grasses	274:280	arg1	LGDZ					241:244	LGDZ	241:244	LGDZ	241:244	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	11	theme	grasses	274:280	arg1	zone					235:238	the leaf growth-and-differentiation zone	199:238	the leaf growth-and-differentiation zone	199:238	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	12	theme	growth-and-differentiation	208:233	arg1	LGDZ					241:244	LGDZ	241:244	LGDZ	241:244	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	12	theme	growth-and-differentiation	208:233	arg1	zone					235:238	the leaf growth-and-differentiation zone	199:238	the leaf growth-and-differentiation zone	199:238	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	3	13	contain	had	886:888	arg2	effect					893:898	no effect	890:898	no effect	890:898	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	3	13	contain	had	886:888	arg1	proportion					777:786	The proportion	773:786	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD	773:884	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	6	14	theme	future	1368:1373	arg1	investigations					1375:1388	future investigations	1368:1388	future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively	1368:1483	The well-constrained px will be useful in future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively.
28369914	2	15	theme	∆18	592:594	arg1	OLGDZ					596:600	∆18 OLGDZ	592:600	∆18 OLGDZ	592:600	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	5	16	theme	VPD	1227:1229	arg1	independent					1212:1222	independent	1212:1222	independent	1212:1222	The isotopic composition of water in the LGDZ was close to that of source water, independent of VPD and much less enriched than previously supposed, but similar to reported xylem water in trees.
28369914	3	17	theme	unenriched	791:800	arg1	water					802:806	unenriched water	791:806	unenriched water (px )	791:812	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	3	17	theme	unenriched	791:800	arg1	px					809:810	px	809:810	px	809:810	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	1	18	theme	biological	464:473	arg1	archives					475:482	these biological archives	458:482	these biological archives	458:482	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	0	19	theme	grasses	75:81	arg1	zone					67:70	the leaf growth-and-differentiation zone	31:70	the leaf growth-and-differentiation zone of grasses	31:81	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	0	20	from	H	17:17	arg1	zone					67:70	the leaf growth-and-differentiation zone	31:70	the leaf growth-and-differentiation zone of grasses	31:81	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	5	21	theme	isotopic	1135:1142	arg1	composition					1144:1154	The isotopic composition	1131:1154	The isotopic composition of water in the LGDZ	1131:1175	The isotopic composition of water in the LGDZ was close to that of source water, independent of VPD and much less enriched than previously supposed, but similar to reported xylem water in trees.
28369914	5	21	theme	isotopic	1135:1142	arg1	that					1190:1193	that	1190:1193	that	1190:1193	The isotopic composition of water in the LGDZ was close to that of source water, independent of VPD and much less enriched than previously supposed, but similar to reported xylem water in trees.
28369914	1	22	theme	important	366:374	arg1	information					433:443	important for understanding (paleo)environmental and physiological information	366:443	wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives -	349:484	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	6	23	theme	oxygen	1393:1398	arg1	investigations					1375:1388	future investigations	1368:1388	future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively	1368:1483	The well-constrained px will be useful in future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively.
28369914	3	24	theme	species	967:973	arg1	effects					975:981	species effects	967:981	species effects	967:981	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	0	25	theme	δ18	4:6	arg1	O					8:8	The δ18 O	0:8	The δ18 O	0:8	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	0	25	theme	δ18	4:6	arg1	water					102:106	source water	95:106	source water in both humid and dry atmospheres	95:140	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	4	26	theme	Deuterium	1013:1021	arg1	discrimination					1023:1036	Deuterium discrimination	1013:1036	Deuterium discrimination	1013:1036	Deuterium discrimination caused depletion of 2 H in LGDZ water, increasing (apparent) px -values > 1.0 in some cases.
28369914	6	27	theme	hydrogen	1404:1411	arg1	fractionation					1422:1434	hydrogen isotopic fractionation	1404:1434	hydrogen isotopic fractionation	1404:1434	The well-constrained px will be useful in future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively.
28369914	5	28	from	composition	1144:1154	arg1	LGDZ					1172:1175	the LGDZ	1168:1175	the LGDZ	1168:1175	The isotopic composition of water in the LGDZ was close to that of source water, independent of VPD and much less enriched than previously supposed, but similar to reported xylem water in trees.
28369914	1	29	dep	wax	349:351	arg1	information					433:443	important for understanding (paleo)environmental and physiological information	366:443	wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives -	349:484	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	2	30	theme	high	730:733	arg1	VPD					767:769	VPD	767:769	VPD	767:769	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	30	theme	high	730:733	arg1	deficit					758:764	high and low vapor pressure deficit	730:764	high and low vapor pressure deficit (VPD)	730:770	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	5	31	theme	reported	1295:1302	arg1	water					1310:1314	reported xylem water	1295:1314	reported xylem water in trees	1295:1323	The isotopic composition of water in the LGDZ was close to that of source water, independent of VPD and much less enriched than previously supposed, but similar to reported xylem water in trees.
28369914	2	32	dep	water	665:669	arg1	OLW					676:678	∆18 OLW	672:678	∆18 OLW	672:678	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	32	dep	water	665:669	arg1	HLW					684:686	∆2 HLW	681:686	∆2 HLW	681:686	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	33	dep	LGDZ	586:589	arg1	OLGDZ					596:600	∆18 OLGDZ	592:600	∆18 OLGDZ	592:600	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	33	dep	LGDZ	586:589	arg1	HLGDZ					609:613	∆2 HLGDZ	606:613	∆2 HLGDZ	606:613	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	6	34	theme	fractionation	1422:1434	arg1	investigations					1375:1388	future investigations	1368:1388	future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively	1368:1483	The well-constrained px will be useful in future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively.
28369914	3	35	theme	O-	924:925	arg1	proportion					907:916	the proportion	903:916	the proportion of 18 O- and 2 H-enriched water in the LGDZ	903:960	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	4	36	theme	apparent	1089:1096	arg1	-values					1102:1108	(apparent) px -values > 1.0	1088:1114	(apparent) px -values > 1.0 in some cases	1088:1128	Deuterium discrimination caused depletion of 2 H in LGDZ water, increasing (apparent) px -values > 1.0 in some cases.
28369914	5	37	theme	source	1198:1203	arg1	water					1205:1209	source water	1198:1209	source water	1198:1209	The isotopic composition of water in the LGDZ was close to that of source water, independent of VPD and much less enriched than previously supposed, but similar to reported xylem water in trees.
28369914	2	38	theme	C4	710:711	arg1	grasses					713:719	two C3 and three C4 grasses	693:719	grasses	713:719	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	1	39	theme	leaf	321:324	arg1	oxygen					337:342	leaf cellulose (oxygen)	321:343	leaf cellulose (oxygen)	321:343	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	3	40	theme	water	944:948	arg1	proportion					907:916	the proportion	903:916	the proportion of 18 O- and 2 H-enriched water in the LGDZ	903:960	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	3	41	from	proportion	777:786	arg1	LGDZ					821:824	the LGDZ	817:824	the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD	817:884	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	1	42	theme	hydrogen	158:165	arg1	composition					175:185	The oxygen and hydrogen isotope composition	143:185	composition	175:185	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	3	43	dep	1.0	845:847	arg1	to					842:843	to	842:843	to	842:843	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	2	44	theme	vapor	743:747	arg1	VPD					767:769	VPD	767:769	VPD	767:769	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	44	theme	vapor	743:747	arg1	deficit					758:764	high and low vapor pressure deficit	730:764	high and low vapor pressure deficit (VPD)	730:770	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	0	45	theme	source	95:100	arg1	O					8:8	The δ18 O	0:8	The δ18 O	0:8	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	0	45	theme	source	95:100	arg1	water					102:106	source water	95:106	source water in both humid and dry atmospheres	95:140	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	1	46	theme	cellulose	326:334	arg1	oxygen					337:342	leaf cellulose (oxygen)	321:343	leaf cellulose (oxygen)	321:343	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	2	47	dep	O-	629:630	arg1	the					622:624	the	622:624	the	622:624	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	1	48	theme	isotope	167:173	arg1	composition					175:185	The oxygen and hydrogen isotope composition	143:185	composition	175:185	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	0	49	theme	water	22:26	arg1	O					8:8	The δ18 O	0:8	The δ18 O	0:8	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	0	49	theme	water	22:26	arg1	H					17:17	δ2 H	14:17	δ2 H of water	14:26	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	0	49	theme	water	22:26	arg1	water					102:106	source water	95:106	source water in both humid and dry atmospheres	95:140	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	2	50	theme	low	739:741	arg1	VPD					767:769	VPD	767:769	VPD	767:769	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	50	theme	low	739:741	arg1	deficit					758:764	high and low vapor pressure deficit	730:764	high and low vapor pressure deficit (VPD)	730:770	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	6	51	theme	wax	1457:1459	arg1	synthesis					1461:1469	wax synthesis	1457:1469	wax synthesis	1457:1469	The well-constrained px will be useful in future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively.
28369914	1	52	theme	isotopic	297:304	arg1	composition					306:316	the isotopic composition	293:316	the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives -	293:484	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	2	53	theme	pressure	749:756	arg1	VPD					767:769	VPD	767:769	VPD	767:769	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	53	theme	pressure	749:756	arg1	deficit					758:764	high and low vapor pressure deficit	730:764	high and low vapor pressure deficit (VPD)	730:770	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	6	54	theme	isotopic	1413:1420	arg1	fractionation					1422:1434	hydrogen isotopic fractionation	1404:1434	hydrogen isotopic fractionation	1404:1434	The well-constrained px will be useful in future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively.
28369914	3	55	theme	H.	879:880	arg1	VPD					882:884	2 H. VPD	877:884	2 H. VPD	877:884	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	1	56	theme	oxygen	337:342	arg1	composition					306:316	the isotopic composition	293:316	the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives -	293:484	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	57	from	oxygen	147:152	arg1	LGDZ					241:244	LGDZ	241:244	LGDZ	241:244	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	57	from	oxygen	147:152	arg1	zone					235:238	the leaf growth-and-differentiation zone	199:238	the leaf growth-and-differentiation zone	199:238	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	3	58	from	proportion	907:916	arg1	LGDZ					957:960	the LGDZ	953:960	the LGDZ	953:960	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	2	59	theme	water	665:669	arg1	HLGDZ					550:554	δ18 OLGDZ and δ2 HLGDZ	533:554	HLGDZ	550:554	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	59	theme	water	665:669	arg1	O-					629:630	18 O-	626:630	18 O-	626:630	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	59	theme	water	665:669	arg1	O-					561:562	18 O- and 2 H-enrichment	558:581	O-	561:562	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	59	theme	water	665:669	arg1	H-enrichment					638:649	2 H-enrichment	636:649	2 H-enrichment	636:649	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	59	theme	water	665:669	arg1	OLGDZ					537:541	δ18 OLGDZ and δ2 HLGDZ	533:554	OLGDZ	537:541	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	59	theme	water	665:669	arg1	H-enrichment					570:581	18 O- and 2 H-enrichment	558:581	H-enrichment	570:581	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	1	60	theme	δ2	260:261	arg1	OLGDZ					252:256	δ18 OLGDZ	248:256	δ18 OLGDZ	248:256	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	60	theme	δ2	260:261	arg1	HLGDZ					263:267	δ2 HLGDZ	260:267	δ2 HLGDZ	260:267	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	61	from	composition	175:185	arg1	LGDZ					241:244	LGDZ	241:244	LGDZ	241:244	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	61	from	composition	175:185	arg1	zone					235:238	the leaf growth-and-differentiation zone	199:238	the leaf growth-and-differentiation zone	199:238	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	62	dep	zone	235:238	arg1	OLGDZ					252:256	δ18 OLGDZ	248:256	δ18 OLGDZ	248:256	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	62	dep	zone	235:238	arg1	HLGDZ					263:267	δ2 HLGDZ	260:267	δ2 HLGDZ	260:267	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	2	63	theme	LGDZ	586:589	arg1	HLGDZ					550:554	δ18 OLGDZ and δ2 HLGDZ	533:554	HLGDZ	550:554	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	63	theme	LGDZ	586:589	arg1	O-					629:630	18 O-	626:630	18 O-	626:630	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	63	theme	LGDZ	586:589	arg1	O-					561:562	18 O- and 2 H-enrichment	558:581	O-	561:562	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	63	theme	LGDZ	586:589	arg1	H-enrichment					638:649	2 H-enrichment	636:649	2 H-enrichment	636:649	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	63	theme	LGDZ	586:589	arg1	OLGDZ					537:541	δ18 OLGDZ and δ2 HLGDZ	533:554	OLGDZ	537:541	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	2	63	theme	LGDZ	586:589	arg1	H-enrichment					570:581	18 O- and 2 H-enrichment	558:581	H-enrichment	570:581	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	6	64	from	useful	1358:1363	arg1	investigations					1375:1388	future investigations	1368:1388	future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively	1368:1483	The well-constrained px will be useful in future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively.
28369914	2	65	theme	blade	659:663	arg1	water					665:669	leaf blade water	654:669	leaf blade water (∆18 OLW, ∆2 HLW )	654:688	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	0	66	from	water	102:106	arg1	atmospheres					130:140	both humid and dry atmospheres	111:140	both humid and dry atmospheres	111:140	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	0	67	theme	humid	116:120	arg1	atmospheres					130:140	both humid and dry atmospheres	111:140	both humid and dry atmospheres	111:140	The δ18 O and δ2 H of water in the leaf growth-and-differentiation zone of grasses is close to source water in both humid and dry atmospheres.
28369914	4	68	from	-values	1102:1108	arg1	cases					1124:1128	some cases	1119:1128	some cases	1119:1128	Deuterium discrimination caused depletion of 2 H in LGDZ water, increasing (apparent) px -values > 1.0 in some cases.
28369914	1	69	theme	physiological	419:431	arg1	information					433:443	important for understanding (paleo)environmental and physiological information	366:443	wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives -	349:484	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	2	70	theme	leaf	654:657	arg1	water					665:669	leaf blade water	654:669	leaf blade water (∆18 OLW, ∆2 HLW )	654:688	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	1	71	theme	wax	349:351	arg1	composition					306:316	the isotopic composition	293:316	the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives -	293:484	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	6	72	theme	well-constrained	1330:1345	arg1	px					1347:1348	The well-constrained px	1326:1348	The well-constrained px	1326:1348	The well-constrained px will be useful in future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively.
28369914	6	72	theme	well-constrained	1330:1345	arg1	useful					1358:1363	useful	1358:1363	useful	1358:1363	The well-constrained px will be useful in future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively.
28369914	6	73	from	investigations	1375:1388	arg1	px					1347:1348	The well-constrained px	1326:1348	The well-constrained px	1326:1348	The well-constrained px will be useful in future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively.
28369914	6	73	from	investigations	1375:1388	arg1	useful					1358:1363	useful	1358:1363	useful	1358:1363	The well-constrained px will be useful in future investigations of oxygen and hydrogen isotopic fractionation during cellulose and wax synthesis, respectively.
28369914	1	74	theme	water	190:194	arg1	composition					175:185	The oxygen and hydrogen isotope composition	143:185	composition	175:185	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	74	theme	water	190:194	arg1	oxygen					147:152	The oxygen and hydrogen isotope composition	143:185	oxygen	147:152	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	1	74	theme	water	190:194	arg1	unknown					503:509	unknown	503:509	unknown	503:509	The oxygen and hydrogen isotope composition of water in the leaf growth-and-differentiation zone, LGDZ, (δ18 OLGDZ , δ2 HLGDZ ) of grasses influences the isotopic composition of leaf cellulose (oxygen) and wax (hydrogen) - important for understanding (paleo)environmental and physiological information contained in these biological archives - but is presently unknown.
28369914	3	75	theme	H-enriched	933:942	arg1	water					944:948	2 H-enriched water	931:948	2 H-enriched water	931:948	The proportion of unenriched water (px ) in the LGDZ ranged from 0.9 to 1.0 for 18 O and 1.0 to 1.2 for 2 H. VPD had no effect on the proportion of 18 O- and 2 H-enriched water in the LGDZ, and species effects were small or nonsignificant.
28369914	5	76	from	water	1310:1314	arg1	trees					1319:1323	trees	1319:1323	trees	1319:1323	The isotopic composition of water in the LGDZ was close to that of source water, independent of VPD and much less enriched than previously supposed, but similar to reported xylem water in trees.
28369914	5	77	theme	xylem	1304:1308	arg1	water					1310:1314	reported xylem water	1295:1314	reported xylem water in trees	1295:1323	The isotopic composition of water in the LGDZ was close to that of source water, independent of VPD and much less enriched than previously supposed, but similar to reported xylem water in trees.
28369914	2	78	theme	∆2	606:607	arg1	HLGDZ					609:613	∆2 HLGDZ	606:613	∆2 HLGDZ	606:613	This work determined δ18 OLGDZ and δ2 HLGDZ , 18 O- and 2 H-enrichment of LGDZ (∆18 OLGDZ and ∆2 HLGDZ ), and the 18 O- and 2 H-enrichment of leaf blade water (∆18 OLW, ∆2 HLW ) in two C3 and three C4 grasses grown at high and low vapor pressure deficit (VPD).
28369914	4	79	theme	H	1060:1060	arg1	depletion					1045:1053	depletion	1045:1053	depletion of 2 H	1045:1060	Deuterium discrimination caused depletion of 2 H in LGDZ water, increasing (apparent) px -values > 1.0 in some cases.
24299917	5	0	theme	lung	709:712	arg1	histology					714:722	lung histology	709:722	lung histology	709:722	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	6	1	theme	decreased	865:873	arg1	aggregates					892:901	decreased large surfactant aggregates	865:901	decreased large surfactant aggregates	865:901	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	7	2	theme	protein	1087:1093	arg1	content					1095:1101	reduced total protein content	1073:1101	reduced total protein content	1073:1101	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation effectively reduced neutrophil accumulation, preserved alveolar epithelial cells and reduced total protein content in BALF.
24299917	5	3	theme	initial	494:500	arg1	analysis					502:509	initial analysis	494:509	initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein	494:572	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	6	4	from	aggregates	892:901	arg1	lungs					795:799	the lungs	791:799	the lungs	791:799	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	7	5	theme	total	1081:1085	arg1	content					1095:1101	reduced total protein content	1073:1101	reduced total protein content	1073:1101	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation effectively reduced neutrophil accumulation, preserved alveolar epithelial cells and reduced total protein content in BALF.
24299917	5	6	theme	MAC	592:594	arg1	min					599:601	0.5 MAC 15 min	588:601	0.5 MAC 15 min before LPS	588:612	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	5	7	theme	cells	550:554	arg1	analysis					502:509	initial analysis	494:509	initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein	494:572	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	5	8	theme	min	599:601	arg1	group					579:583	the group	575:583	the group of 0.5 MAC 15 min before LPS	575:612	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	5	8	theme	min	599:601	arg1	min					599:601	0.5 MAC 15 min	588:601	0.5 MAC 15 min before LPS	588:612	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	10	9	theme	alteration	1516:1525	arg1	inhibition					1474:1483	inhibition	1474:1483	inhibition of neutrophil accumulation and alteration of the surfactant composition	1474:1555	CONCLUSION Pretreatment with sevoflurane effectively attenuates direct severe lung injury, possibly by inhibition of neutrophil accumulation and alteration of the surfactant composition.
24299917	6	10	theme	macrophages	819:829	arg1	protein					853:859	increased BALF total protein	832:859	increased BALF total protein	832:859	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	10	theme	macrophages	819:829	arg1	neutrophils					762:772	neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates	762:901	neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates	762:901	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	10	theme	macrophages	819:829	arg1	aggregates					892:901	decreased large surfactant aggregates	865:901	decreased large surfactant aggregates	865:901	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	10	theme	macrophages	819:829	arg1	loss					802:805	loss	802:805	loss of alveolar macrophages	802:829	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	10	theme	macrophages	819:829	arg1	sequestration					774:786	sequestration	774:786	sequestration in the lungs	774:799	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	7	11	theme	Only	904:907	arg1	inhalation					909:918	Only inhalation	904:918	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation	904:986	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation effectively reduced neutrophil accumulation, preserved alveolar epithelial cells and reduced total protein content in BALF.
24299917	7	12	theme	reduced	1073:1079	arg1	content					1095:1101	reduced total protein content	1073:1101	reduced total protein content	1073:1101	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation effectively reduced neutrophil accumulation, preserved alveolar epithelial cells and reduced total protein content in BALF.
24299917	1	13	theme	BACKGROUND	61:70	arg1	Sevoflurane					72:82	BACKGROUND Sevoflurane	61:82	BACKGROUND Sevoflurane	61:82	BACKGROUND Sevoflurane exerts effects on pulmonary cells that could protect against lung injury.
24299917	6	14	theme	total	847:851	arg1	neutrophils					762:772	neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates	762:901	neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates	762:901	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	14	theme	total	847:851	arg1	protein					853:859	increased BALF total protein	832:859	increased BALF total protein	832:859	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	4	15	from	MAC	463:465	arg1	hours					470:474	24 hours	467:474	24 hours before LPS	467:485	Sevoflurane was administered for 30 min at 0.25, 0.5 or 1.0 MAC 15 min before LPS or for 30 min at 0.5 MAC 24 hours before LPS.
24299917	4	15	from	MAC	463:465	arg1	min					452:454	30 min	449:454	30 min at 0.5 MAC	449:465	Sevoflurane was administered for 30 min at 0.25, 0.5 or 1.0 MAC 15 min before LPS or for 30 min at 0.5 MAC 24 hours before LPS.
24299917	5	16	theme	total	560:564	arg1	protein					566:572	total protein	560:572	total protein	560:572	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	6	17	dep	RESULTS	725:731	arg1	resulted					750:757	resulted	750:757	resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates	750:901	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	5	18	theme	plasma	675:680	arg1	levels					698:703	plasma malondialdehyde levels	675:703	plasma malondialdehyde levels	675:703	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	6	19	theme	BALF	842:845	arg1	neutrophils					762:772	neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates	762:901	neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates	762:901	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	19	theme	BALF	842:845	arg1	protein					853:859	increased BALF total protein	832:859	increased BALF total protein	832:859	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	10	20	theme	accumulation	1499:1510	arg1	inhibition					1474:1483	inhibition	1474:1483	inhibition of neutrophil accumulation and alteration of the surfactant composition	1474:1555	CONCLUSION Pretreatment with sevoflurane effectively attenuates direct severe lung injury, possibly by inhibition of neutrophil accumulation and alteration of the surfactant composition.
24299917	5	21	theme	protein	566:572	arg1	analysis					502:509	initial analysis	494:509	initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein	494:572	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	5	22	theme	malondialdehyde	682:696	arg1	levels					698:703	plasma malondialdehyde levels	675:703	plasma malondialdehyde levels	675:703	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	6	23	theme	increased	832:840	arg1	neutrophils					762:772	neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates	762:901	neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates	762:901	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	23	theme	increased	832:840	arg1	protein					853:859	increased BALF total protein	832:859	increased BALF total protein	832:859	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	8	24	theme	surfactant	1188:1197	arg1	aggregates					1199:1208	large surfactant aggregates	1182:1208	large surfactant aggregates	1182:1208	This regimen also reduced plasma malondialdehyde levels and increased large surfactant aggregates, despite the application of mechanical ventilation.
24299917	5	25	theme	fluid	537:541	arg1	cells					550:554	bronchoalveolar lavage fluid (BALF) cells	514:554	bronchoalveolar lavage fluid (BALF) cells	514:554	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	6	26	from	loss	802:805	arg1	lungs					795:799	the lungs	791:799	the lungs	791:799	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	8	27	theme	plasma	1138:1143	arg1	levels					1161:1166	plasma malondialdehyde levels	1138:1166	plasma malondialdehyde levels	1138:1166	This regimen also reduced plasma malondialdehyde levels and increased large surfactant aggregates, despite the application of mechanical ventilation.
24299917	7	28	dep	min	942:944	arg1	min					960:962	15 min	957:962	30 min at 0.5 MAC 15 min before LPS installation	939:986	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation effectively reduced neutrophil accumulation, preserved alveolar epithelial cells and reduced total protein content in BALF.
24299917	7	29	theme	alveolar	1043:1050	arg1	cells					1063:1067	alveolar epithelial cells	1043:1067	alveolar epithelial cells	1043:1067	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation effectively reduced neutrophil accumulation, preserved alveolar epithelial cells and reduced total protein content in BALF.
24299917	6	30	theme	LPS	733:735	arg1	instillation					737:748	LPS instillation	733:748	LPS instillation	733:748	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	9	31	theme	LPS	1294:1296	arg1	instillation					1298:1309	LPS instillation	1294:1309	LPS instillation	1294:1309	This effect was preserved after LPS instillation and the favorable composition of surfactant was maintained.
24299917	6	32	from	sequestration	774:786	arg1	lungs					795:799	the lungs	791:799	the lungs	791:799	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	3	33	theme	METHODS	277:283	arg1	LPS					285:287	METHODS LPS	277:287	METHODS LPS	277:287	METHODS LPS was administered intratracheally in Wistar rats to induce lung injury.
24299917	9	34	theme	favorable	1319:1327	arg1	composition					1329:1339	the favorable composition	1315:1339	the favorable composition of surfactant	1315:1353	This effect was preserved after LPS instillation and the favorable composition of surfactant was maintained.
24299917	7	35	theme	epithelial	1052:1061	arg1	cells					1063:1067	alveolar epithelial cells	1043:1067	alveolar epithelial cells	1043:1067	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation effectively reduced neutrophil accumulation, preserved alveolar epithelial cells and reduced total protein content in BALF.
24299917	10	36	theme	direct	1435:1440	arg1	injury					1454:1459	direct severe lung injury	1435:1459	direct severe lung injury	1435:1459	CONCLUSION Pretreatment with sevoflurane effectively attenuates direct severe lung injury, possibly by inhibition of neutrophil accumulation and alteration of the surfactant composition.
24299917	1	37	theme	pulmonary	102:110	arg1	cells					112:116	pulmonary cells	102:116	pulmonary cells that could protect against lung injury	102:155	BACKGROUND Sevoflurane exerts effects on pulmonary cells that could protect against lung injury.
24299917	7	38	theme	sevoflurane	923:933	arg1	inhalation					909:918	Only inhalation	904:918	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation	904:986	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation effectively reduced neutrophil accumulation, preserved alveolar epithelial cells and reduced total protein content in BALF.
24299917	7	39	from	MAC	953:955	arg1	min					942:944	30 min	939:944	30 min at 0.5 MAC 15 min before LPS installation	939:986	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation effectively reduced neutrophil accumulation, preserved alveolar epithelial cells and reduced total protein content in BALF.
24299917	7	40	theme	neutrophil	1008:1017	arg1	accumulation					1019:1030	neutrophil accumulation	1008:1030	neutrophil accumulation	1008:1030	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation effectively reduced neutrophil accumulation, preserved alveolar epithelial cells and reduced total protein content in BALF.
24299917	2	41	theme	pretreatment	188:199	arg1	potential					175:183	the potential	171:183	the potential of pretreatment with sevoflurane to attenuate lipopolysaccharide (LPS)-induced lung injury	171:274	We evaluated the potential of pretreatment with sevoflurane to attenuate lipopolysaccharide (LPS)-induced lung injury.
24299917	8	42	theme	mechanical	1238:1247	arg1	ventilation					1249:1259	mechanical ventilation	1238:1259	mechanical ventilation	1238:1259	This regimen also reduced plasma malondialdehyde levels and increased large surfactant aggregates, despite the application of mechanical ventilation.
24299917	0	43	theme	lung	48:51	arg1	injury					53:58	direct lung injury	41:58	direct lung injury	41:58	Pretreatment with sevoflurane attenuates direct lung injury.
24299917	9	44	theme	surfactant	1344:1353	arg1	composition					1329:1339	the favorable composition	1315:1339	the favorable composition of surfactant	1315:1353	This effect was preserved after LPS instillation and the favorable composition of surfactant was maintained.
24299917	5	45	theme	surfactant	639:648	arg1	subfractions					661:672	surfactant aggregates subfractions	639:672	surfactant aggregates subfractions	639:672	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	6	46	theme	alveolar	810:817	arg1	macrophages					819:829	alveolar macrophages	810:829	alveolar macrophages	810:829	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	47	dep	neutrophils	762:772	arg1	neutrophils					762:772	neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates	762:901	neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates	762:901	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	47	dep	neutrophils	762:772	arg1	loss					802:805	loss	802:805	loss of alveolar macrophages	802:829	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	47	dep	neutrophils	762:772	arg1	aggregates					892:901	decreased large surfactant aggregates	865:901	decreased large surfactant aggregates	865:901	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	47	dep	neutrophils	762:772	arg1	protein					853:859	increased BALF total protein	832:859	increased BALF total protein	832:859	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	47	dep	neutrophils	762:772	arg1	sequestration					774:786	sequestration	774:786	sequestration in the lungs	774:799	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	0	48	theme	direct	41:46	arg1	injury					53:58	direct lung injury	41:58	direct lung injury	41:58	Pretreatment with sevoflurane attenuates direct lung injury.
24299917	5	49	theme	aggregates	650:659	arg1	subfractions					661:672	surfactant aggregates subfractions	639:672	surfactant aggregates subfractions	639:672	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	10	50	theme	severe	1442:1447	arg1	injury					1454:1459	direct severe lung injury	1435:1459	direct severe lung injury	1435:1459	CONCLUSION Pretreatment with sevoflurane effectively attenuates direct severe lung injury, possibly by inhibition of neutrophil accumulation and alteration of the surfactant composition.
24299917	3	51	theme	Wistar	325:330	arg1	rats					332:335	Wistar rats	325:335	Wistar rats	325:335	METHODS LPS was administered intratracheally in Wistar rats to induce lung injury.
24299917	10	52	with	Pretreatment	1382:1393	arg1	sevoflurane					1400:1410	sevoflurane	1400:1410	sevoflurane	1400:1410	CONCLUSION Pretreatment with sevoflurane effectively attenuates direct severe lung injury, possibly by inhibition of neutrophil accumulation and alteration of the surfactant composition.
24299917	10	53	theme	composition	1545:1555	arg1	alteration					1516:1525	alteration	1516:1525	alteration of the surfactant composition	1516:1555	CONCLUSION Pretreatment with sevoflurane effectively attenuates direct severe lung injury, possibly by inhibition of neutrophil accumulation and alteration of the surfactant composition.
24299917	10	53	theme	composition	1545:1555	arg1	accumulation					1499:1510	neutrophil accumulation	1488:1510	neutrophil accumulation	1488:1510	CONCLUSION Pretreatment with sevoflurane effectively attenuates direct severe lung injury, possibly by inhibition of neutrophil accumulation and alteration of the surfactant composition.
24299917	10	54	theme	neutrophil	1488:1497	arg1	accumulation					1499:1510	neutrophil accumulation	1488:1510	neutrophil accumulation	1488:1510	CONCLUSION Pretreatment with sevoflurane effectively attenuates direct severe lung injury, possibly by inhibition of neutrophil accumulation and alteration of the surfactant composition.
24299917	5	55	theme	bronchoalveolar	514:528	arg1	BALF					544:547	BALF	544:547	BALF	544:547	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	5	55	theme	bronchoalveolar	514:528	arg1	fluid					537:541	bronchoalveolar lavage fluid	514:541	bronchoalveolar lavage fluid (BALF) cells	514:554	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	6	56	theme	surfactant	881:890	arg1	aggregates					892:901	decreased large surfactant aggregates	865:901	decreased large surfactant aggregates	865:901	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	7	57	theme	LPS	971:973	arg1	installation					975:986	LPS installation	971:986	LPS installation	971:986	Only inhalation of sevoflurane for 30 min at 0.5 MAC 15 min before LPS installation effectively reduced neutrophil accumulation, preserved alveolar epithelial cells and reduced total protein content in BALF.
24299917	10	58	theme	lung	1449:1452	arg1	injury					1454:1459	direct severe lung injury	1435:1459	direct severe lung injury	1435:1459	CONCLUSION Pretreatment with sevoflurane effectively attenuates direct severe lung injury, possibly by inhibition of neutrophil accumulation and alteration of the surfactant composition.
24299917	0	59	with	Pretreatment	0:11	arg1	sevoflurane					18:28	sevoflurane	18:28	sevoflurane	18:28	Pretreatment with sevoflurane attenuates direct lung injury.
24299917	2	60	with	pretreatment	188:199	arg1	sevoflurane					206:216	sevoflurane	206:216	sevoflurane	206:216	We evaluated the potential of pretreatment with sevoflurane to attenuate lipopolysaccharide (LPS)-induced lung injury.
24299917	4	61	dep	min	427:429	arg1	MAC					420:422	MAC	420:422	MAC	420:422	Sevoflurane was administered for 30 min at 0.25, 0.5 or 1.0 MAC 15 min before LPS or for 30 min at 0.5 MAC 24 hours before LPS.
24299917	5	62	theme	lavage	530:535	arg1	BALF					544:547	BALF	544:547	BALF	544:547	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	5	62	theme	lavage	530:535	arg1	fluid					537:541	bronchoalveolar lavage fluid	514:541	bronchoalveolar lavage fluid (BALF) cells	514:554	After initial analysis of bronchoalveolar lavage fluid (BALF) cells and total protein, the group of 0.5 MAC 15 min before LPS was further analyzed for surfactant aggregates subfractions, plasma malondialdehyde levels and lung histology.
24299917	8	63	theme	malondialdehyde	1145:1159	arg1	levels					1161:1166	plasma malondialdehyde levels	1138:1166	plasma malondialdehyde levels	1138:1166	This regimen also reduced plasma malondialdehyde levels and increased large surfactant aggregates, despite the application of mechanical ventilation.
24299917	10	64	theme	CONCLUSION	1371:1380	arg1	Pretreatment					1382:1393	CONCLUSION Pretreatment	1371:1393	CONCLUSION Pretreatment with sevoflurane	1371:1410	CONCLUSION Pretreatment with sevoflurane effectively attenuates direct severe lung injury, possibly by inhibition of neutrophil accumulation and alteration of the surfactant composition.
24299917	1	65	theme	lung	145:148	arg1	injury					150:155	lung injury	145:155	lung injury	145:155	BACKGROUND Sevoflurane exerts effects on pulmonary cells that could protect against lung injury.
24299917	8	66	theme	large	1182:1186	arg1	aggregates					1199:1208	large surfactant aggregates	1182:1208	large surfactant aggregates	1182:1208	This regimen also reduced plasma malondialdehyde levels and increased large surfactant aggregates, despite the application of mechanical ventilation.
24299917	3	67	theme	lung	347:350	arg1	injury					352:357	lung injury	347:357	lung injury	347:357	METHODS LPS was administered intratracheally in Wistar rats to induce lung injury.
24299917	2	68	theme	lung	264:267	arg1	injury					269:274	lipopolysaccharide (LPS)-induced lung injury	231:274	lipopolysaccharide (LPS)-induced lung injury	231:274	We evaluated the potential of pretreatment with sevoflurane to attenuate lipopolysaccharide (LPS)-induced lung injury.
24299917	8	69	theme	ventilation	1249:1259	arg1	application					1223:1233	the application	1219:1233	the application of mechanical ventilation	1219:1259	This regimen also reduced plasma malondialdehyde levels and increased large surfactant aggregates, despite the application of mechanical ventilation.
24299917	4	70	dep	MAC	420:422	arg1	1.0					416:418	1.0	416:418	1.0	416:418	Sevoflurane was administered for 30 min at 0.25, 0.5 or 1.0 MAC 15 min before LPS or for 30 min at 0.5 MAC 24 hours before LPS.
24299917	4	70	dep	MAC	420:422	arg1	0.5					409:411	0.5	409:411	0.5	409:411	Sevoflurane was administered for 30 min at 0.25, 0.5 or 1.0 MAC 15 min before LPS or for 30 min at 0.5 MAC 24 hours before LPS.
24299917	6	71	from	protein	853:859	arg1	lungs					795:799	the lungs	791:799	the lungs	791:799	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	6	72	theme	large	875:879	arg1	aggregates					892:901	decreased large surfactant aggregates	865:901	decreased large surfactant aggregates	865:901	RESULTS LPS instillation resulted in neutrophils sequestration in the lungs, loss of alveolar macrophages, increased BALF total protein and decreased large surfactant aggregates.
24299917	2	73	theme	-induced	255:262	arg1	injury					269:274	lipopolysaccharide (LPS)-induced lung injury	231:274	lipopolysaccharide (LPS)-induced lung injury	231:274	We evaluated the potential of pretreatment with sevoflurane to attenuate lipopolysaccharide (LPS)-induced lung injury.
24299917	10	74	theme	surfactant	1534:1543	arg1	composition					1545:1555	the surfactant composition	1530:1555	the surfactant composition	1530:1555	CONCLUSION Pretreatment with sevoflurane effectively attenuates direct severe lung injury, possibly by inhibition of neutrophil accumulation and alteration of the surfactant composition.
27349789	1	0	theme	high	162:165	arg1	specificity					167:177	high specificity	162:177	high specificity	162:177	NEL-like molecule-1 (NELL-1) is a novel osteogenic protein that showing high specificity to osteochondral cells.
27349789	2	1	used	used	217:220	arg2	It					203:204	It	203:204	It	203:204	It was widely used in bone regeneration research by loading onto carriers such as tricalcium phosphate (TCP) particles.
27349789	3	2	from	material	403:410	arg1	application					430:440	its potential application	416:440	its potential application	416:440	However, there has been little research on protein controlled release from this material and its potential application.
27349789	3	2	from	material	403:410	arg1	release					385:391	protein controlled release	366:391	protein controlled release from this material	366:410	However, there has been little research on protein controlled release from this material and its potential application.
27349789	4	3	mod	modified	472:479	arg3	coating					503:509	a hydroxyapatite coating	486:509	a hydroxyapatite coating followed by a chitosan coating to prepare chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP)	486:609	In this study, TCP was first modified with a hydroxyapatite coating followed by a chitosan coating to prepare chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP).
27349789	4	3	mod	modified	472:479	arg1	TCP					458:460	TCP	458:460	TCP	458:460	In this study, TCP was first modified with a hydroxyapatite coating followed by a chitosan coating to prepare chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP).
27349789	3	4	from	research	354:361	arg1	application					430:440	its potential application	416:440	its potential application	416:440	However, there has been little research on protein controlled release from this material and its potential application.
27349789	3	4	from	research	354:361	arg1	release					385:391	protein controlled release	366:391	protein controlled release from this material	366:410	However, there has been little research on protein controlled release from this material and its potential application.
27349789	4	5	theme	TCP	584:586	arg1	Chi/HA-TCP					599:608	Chi/HA-TCP	599:608	Chi/HA-TCP	599:608	In this study, TCP was first modified with a hydroxyapatite coating followed by a chitosan coating to prepare chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP).
27349789	4	5	theme	TCP	584:586	arg1	particles					588:596	chitosan/hydroxyapatite-coated TCP particles	553:596	chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP)	553:609	In this study, TCP was first modified with a hydroxyapatite coating followed by a chitosan coating to prepare chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP).
27349789	3	6	theme	little	347:352	arg1	research					354:361	little research	347:361	little research	347:361	However, there has been little research on protein controlled release from this material and its potential application.
27349789	4	7	theme	hydroxyapatite	488:501	arg1	coating					503:509	a hydroxyapatite coating	486:509	a hydroxyapatite coating followed by a chitosan coating to prepare chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP)	486:609	In this study, TCP was first modified with a hydroxyapatite coating followed by a chitosan coating to prepare chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP).
27349789	2	8	theme	phosphate	296:304	arg1	particles					312:320	tricalcium phosphate (TCP) particles	285:320	tricalcium phosphate (TCP) particles	285:320	It was widely used in bone regeneration research by loading onto carriers such as tricalcium phosphate (TCP) particles.
27349789	7	9	theme	0.3	862:864	arg1	%					865:865	%	865:865	%	865:865	It was observed that the Chi/HA-TCP particles prepared with the 0.3% (wt/wt) chitosan solution were able to successfully control the release of NELL-1 and maintain a slow, steady release for up to 28 days.
27349789	9	10	theme	release	1347:1353	arg1	pattern					1355:1361	a sustained release pattern	1335:1361	a sustained release pattern	1335:1361	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	9	11	theme	bone	1581:1584	arg1	research					1599:1606	bone regeneration research	1581:1606	bone regeneration research	1581:1606	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	2	12	theme	tricalcium	285:294	arg1	TCP					307:309	TCP	307:309	TCP	307:309	It was widely used in bone regeneration research by loading onto carriers such as tricalcium phosphate (TCP) particles.
27349789	2	12	theme	tricalcium	285:294	arg1	phosphate					296:304	tricalcium phosphate	285:304	tricalcium phosphate (TCP) particles	285:320	It was widely used in bone regeneration research by loading onto carriers such as tricalcium phosphate (TCP) particles.
27349789	7	13	dep	slow	964:967	arg1	steady					970:975	steady	970:975	steady	970:975	It was observed that the Chi/HA-TCP particles prepared with the 0.3% (wt/wt) chitosan solution were able to successfully control the release of NELL-1 and maintain a slow, steady release for up to 28 days.
27349789	9	14	theme	NELL-1	1436:1441	arg1	composite					1454:1462	the NELL-1 integrated composite	1432:1462	the NELL-1 integrated composite of Chi/HA-TCP	1432:1476	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	9	14	theme	NELL-1	1436:1441	arg1	Chi/HA-TCP					1467:1476	Chi/HA-TCP	1467:1476	Chi/HA-TCP	1467:1476	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	9	15	theme	regeneration	1586:1597	arg1	research					1599:1606	bone regeneration research	1581:1606	bone regeneration research	1581:1606	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	1	16	theme	NEL-like	90:97	arg1	NELL-1					111:116	NELL-1	111:116	NELL-1	111:116	NEL-like molecule-1 (NELL-1) is a novel osteogenic protein that showing high specificity to osteochondral cells.
27349789	1	16	theme	NEL-like	90:97	arg1	molecule-1					99:108	NEL-like molecule-1	90:108	NEL-like molecule-1 (NELL-1)	90:117	NEL-like molecule-1 (NELL-1) is a novel osteogenic protein that showing high specificity to osteochondral cells.
27349789	1	16	theme	NEL-like	90:97	arg1	protein					141:147	a novel osteogenic protein	122:147	a novel osteogenic protein that showing high specificity to osteochondral cells	122:200	NEL-like molecule-1 (NELL-1) is a novel osteogenic protein that showing high specificity to osteochondral cells.
27349789	7	17	dep	28	995:996	arg1	to					992:993	to	992:993	to	992:993	It was observed that the Chi/HA-TCP particles prepared with the 0.3% (wt/wt) chitosan solution were able to successfully control the release of NELL-1 and maintain a slow, steady release for up to 28 days.
27349789	9	18	theme	integrated	1443:1452	arg1	composite					1454:1462	the NELL-1 integrated composite	1432:1462	the NELL-1 integrated composite of Chi/HA-TCP	1432:1476	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	9	18	theme	integrated	1443:1452	arg1	Chi/HA-TCP					1467:1476	Chi/HA-TCP	1467:1476	Chi/HA-TCP	1467:1476	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	9	19	theme	protein	1313:1319	arg1	NELL-1					1321:1326	the osteogenic protein NELL-1	1298:1326	the osteogenic protein NELL-1	1298:1326	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	7	20	theme	Chi/HA-TCP	823:832	arg1	particles					834:842	the Chi/HA-TCP particles	819:842	the Chi/HA-TCP particles prepared with the 0.3% (wt/wt) chitosan solution	819:891	It was observed that the Chi/HA-TCP particles prepared with the 0.3% (wt/wt) chitosan solution were able to successfully control the release of NELL-1 and maintain a slow, steady release for up to 28 days.
27349789	7	20	theme	Chi/HA-TCP	823:832	arg1	able					898:901	able	898:901	able	898:901	It was observed that the Chi/HA-TCP particles prepared with the 0.3% (wt/wt) chitosan solution were able to successfully control the release of NELL-1 and maintain a slow, steady release for up to 28 days.
27349789	1	21	theme	osteochondral	182:194	arg1	cells					196:200	osteochondral cells	182:200	osteochondral cells	182:200	NEL-like molecule-1 (NELL-1) is a novel osteogenic protein that showing high specificity to osteochondral cells.
27349789	3	22	theme	controlled	374:383	arg1	release					385:391	protein controlled release	366:391	protein controlled release from this material	366:410	However, there has been little research on protein controlled release from this material and its potential application.
27349789	0	23	theme	Controlled	0:9	arg1	release					11:17	Controlled release	0:17	Controlled release of NELL-1 protein from chitosan/hydroxyapatite-modified TCP particles	0:87	Controlled release of NELL-1 protein from chitosan/hydroxyapatite-modified TCP particles.
27349789	8	24	attach	released	1199:1206	arg2	protein					1191:1197	the protein	1187:1197	the protein released from hydroxyapatite coated TCP (HA-TCP) particles	1187:1256	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	8	24	attach	released	1199:1206	arg1	particles					1248:1256	hydroxyapatite coated TCP (HA-TCP) particles	1213:1256	hydroxyapatite coated TCP (HA-TCP) particles	1213:1256	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	7	25	dep	%	865:865	arg1	wt/wt					868:872	wt/wt	868:872	wt/wt	868:872	It was observed that the Chi/HA-TCP particles prepared with the 0.3% (wt/wt) chitosan solution were able to successfully control the release of NELL-1 and maintain a slow, steady release for up to 28 days.
27349789	3	26	theme	potential	420:428	arg1	application					430:440	its potential application	416:440	its potential application	416:440	However, there has been little research on protein controlled release from this material and its potential application.
27349789	0	27	theme	NELL-1	22:27	arg1	protein					29:35	NELL-1 protein	22:35	NELL-1 protein	22:35	Controlled release of NELL-1 protein from chitosan/hydroxyapatite-modified TCP particles.
27349789	6	28	theme	release	753:759	arg1	kinetics					761:768	the release kinetics	749:768	the release kinetics	749:768	The NELL-1 loaded Chi/HA-TCP particles and the release kinetics were investigated in vitro.
27349789	3	29	theme	protein	366:372	arg1	release					385:391	protein controlled release	366:391	protein controlled release from this material	366:410	However, there has been little research on protein controlled release from this material and its potential application.
27349789	4	30	theme	chitosan/hydroxyapatite-coated	553:582	arg1	Chi/HA-TCP					599:608	Chi/HA-TCP	599:608	Chi/HA-TCP	599:608	In this study, TCP was first modified with a hydroxyapatite coating followed by a chitosan coating to prepare chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP).
27349789	4	30	theme	chitosan/hydroxyapatite-coated	553:582	arg1	particles					588:596	chitosan/hydroxyapatite-coated TCP particles	553:596	chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP)	553:609	In this study, TCP was first modified with a hydroxyapatite coating followed by a chitosan coating to prepare chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP).
27349789	9	31	theme	protein	1514:1520	arg1	carrier					1531:1537	a protein delivery carrier	1512:1537	a protein delivery carrier	1512:1537	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	9	32	theme	Chi/HA-TCP	1467:1476	arg1	composite					1454:1462	the NELL-1 integrated composite	1432:1462	the NELL-1 integrated composite of Chi/HA-TCP	1432:1476	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	9	32	theme	Chi/HA-TCP	1467:1476	arg1	Chi/HA-TCP					1467:1476	Chi/HA-TCP	1467:1476	Chi/HA-TCP	1467:1476	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	9	33	theme	modified	1397:1404	arg1	particles					1417:1425	the modified Chi/HA-TCP particles	1393:1425	the modified Chi/HA-TCP particles	1393:1425	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	5	34	theme	potential	682:690	arg1	measurements					692:703	potential measurements	682:703	potential measurements	682:703	The preparation was characterized by SEM, EDX, FTIR, XRD, FM and Zeta potential measurements.
27349789	9	35	theme	delivery	1522:1529	arg1	carrier					1531:1537	a protein delivery carrier	1512:1537	a protein delivery carrier	1512:1537	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	9	36	theme	Chi/HA-TCP	1406:1415	arg1	particles					1417:1425	the modified Chi/HA-TCP particles	1393:1425	the modified Chi/HA-TCP particles	1393:1425	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	0	37	theme	protein	29:35	arg1	release					11:17	Controlled release	0:17	Controlled release of NELL-1 protein from chitosan/hydroxyapatite-modified TCP particles	0:87	Controlled release of NELL-1 protein from chitosan/hydroxyapatite-modified TCP particles.
27349789	5	38	dep	SEM	649:651	arg1	measurements					692:703	potential measurements	682:703	potential measurements	682:703	The preparation was characterized by SEM, EDX, FTIR, XRD, FM and Zeta potential measurements.
27349789	2	39	theme	regeneration	230:241	arg1	research					243:250	bone regeneration research	225:250	bone regeneration research	225:250	It was widely used in bone regeneration research by loading onto carriers such as tricalcium phosphate (TCP) particles.
27349789	0	40	theme	TCP	75:77	arg1	particles					79:87	chitosan/hydroxyapatite-modified TCP particles	42:87	chitosan/hydroxyapatite-modified TCP particles	42:87	Controlled release of NELL-1 protein from chitosan/hydroxyapatite-modified TCP particles.
27349789	8	41	theme	Chi/HA-TCP	1084:1093	arg1	particles					1095:1103	Chi/HA-TCP particles	1084:1103	Chi/HA-TCP particles	1084:1103	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	8	42	theme	investigation	1128:1140	arg1	period					1114:1119	the period	1110:1119	the period	1110:1119	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	8	42	theme	investigation	1128:1140	arg1	higher					1167:1172	higher	1167:1172	higher	1167:1172	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	0	43	from	particles	79:87	arg1	release					11:17	Controlled release	0:17	Controlled release of NELL-1 protein from chitosan/hydroxyapatite-modified TCP particles	0:87	Controlled release of NELL-1 protein from chitosan/hydroxyapatite-modified TCP particles.
27349789	2	44	theme	bone	225:228	arg1	research					243:250	bone regeneration research	225:250	bone regeneration research	225:250	It was widely used in bone regeneration research by loading onto carriers such as tricalcium phosphate (TCP) particles.
27349789	8	45	theme	hydroxyapatite	1213:1226	arg1	particles					1248:1256	hydroxyapatite coated TCP (HA-TCP) particles	1213:1256	hydroxyapatite coated TCP (HA-TCP) particles	1213:1256	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	0	46	theme	chitosan/hydroxyapatite-modified	42:73	arg1	particles					79:87	chitosan/hydroxyapatite-modified TCP particles	42:87	chitosan/hydroxyapatite-modified TCP particles	42:87	Controlled release of NELL-1 protein from chitosan/hydroxyapatite-modified TCP particles.
27349789	4	47	theme	chitosan	525:532	arg1	coating					534:540	a chitosan coating	523:540	a chitosan coating to prepare chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP)	523:609	In this study, TCP was first modified with a hydroxyapatite coating followed by a chitosan coating to prepare chitosan/hydroxyapatite-coated TCP particles (Chi/HA-TCP).
27349789	7	48	theme	slow	964:967	arg1	release					977:983	a slow, steady release	962:983	a slow, steady release for up to 28 days	962:1001	It was observed that the Chi/HA-TCP particles prepared with the 0.3% (wt/wt) chitosan solution were able to successfully control the release of NELL-1 and maintain a slow, steady release for up to 28 days.
27349789	1	49	theme	novel	124:128	arg1	protein					141:147	a novel osteogenic protein	122:147	a novel osteogenic protein that showing high specificity to osteochondral cells	122:200	NEL-like molecule-1 (NELL-1) is a novel osteogenic protein that showing high specificity to osteochondral cells.
27349789	1	49	theme	novel	124:128	arg1	molecule-1					99:108	NEL-like molecule-1	90:108	NEL-like molecule-1 (NELL-1)	90:117	NEL-like molecule-1 (NELL-1) is a novel osteogenic protein that showing high specificity to osteochondral cells.
27349789	7	50	theme	chitosan	875:882	arg1	solution					884:891	the 0.3% (wt/wt) chitosan solution	858:891	the 0.3% (wt/wt) chitosan solution	858:891	It was observed that the Chi/HA-TCP particles prepared with the 0.3% (wt/wt) chitosan solution were able to successfully control the release of NELL-1 and maintain a slow, steady release for up to 28 days.
27349789	9	51	from	use	1574:1576	arg1	research					1599:1606	bone regeneration research	1581:1606	bone regeneration research	1581:1606	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	6	52	theme	Chi/HA-TCP	724:733	arg1	particles					735:743	The NELL-1 loaded Chi/HA-TCP particles	706:743	The NELL-1 loaded Chi/HA-TCP particles	706:743	The NELL-1 loaded Chi/HA-TCP particles and the release kinetics were investigated in vitro.
27349789	1	53	theme	osteogenic	130:139	arg1	protein					141:147	a novel osteogenic protein	122:147	a novel osteogenic protein that showing high specificity to osteochondral cells	122:200	NEL-like molecule-1 (NELL-1) is a novel osteogenic protein that showing high specificity to osteochondral cells.
27349789	1	53	theme	osteogenic	130:139	arg1	molecule-1					99:108	NEL-like molecule-1	90:108	NEL-like molecule-1 (NELL-1)	90:117	NEL-like molecule-1 (NELL-1) is a novel osteogenic protein that showing high specificity to osteochondral cells.
27349789	9	54	theme	osteogenic	1302:1311	arg1	NELL-1					1321:1326	the osteogenic protein NELL-1	1298:1326	the osteogenic protein NELL-1	1298:1326	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	8	55	theme	loaded	1038:1043	arg1	bioactivity					1055:1065	the loaded protein's bioactivity	1034:1065	the loaded protein's bioactivity	1034:1065	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	9	56	theme	sustained	1337:1345	arg1	pattern					1355:1361	a sustained release pattern	1335:1361	a sustained release pattern	1335:1361	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	9	57	theme	improved	1549:1556	arg1	matrix					1563:1568	an improved bone matrix	1546:1568	an improved bone matrix for use in bone regeneration research	1546:1606	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	6	58	theme	loaded	717:722	arg1	particles					735:743	The NELL-1 loaded Chi/HA-TCP particles	706:743	The NELL-1 loaded Chi/HA-TCP particles	706:743	The NELL-1 loaded Chi/HA-TCP particles and the release kinetics were investigated in vitro.
27349789	8	59	theme	coated	1228:1233	arg1	particles					1248:1256	hydroxyapatite coated TCP (HA-TCP) particles	1213:1256	hydroxyapatite coated TCP (HA-TCP) particles	1213:1256	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	8	60	theme	TCP	1235:1237	arg1	particles					1248:1256	hydroxyapatite coated TCP (HA-TCP) particles	1213:1256	hydroxyapatite coated TCP (HA-TCP) particles	1213:1256	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	8	61	theme	bioactivity	1055:1065	arg1	%					1029:1029	more than 78%	1017:1029	more than 78% of the loaded protein's bioactivity	1017:1065	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	8	61	theme	bioactivity	1055:1065	arg1	bioactivity					1055:1065	the loaded protein's bioactivity	1034:1065	the loaded protein's bioactivity	1034:1065	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	9	62	theme	bone	1558:1561	arg1	matrix					1563:1568	an improved bone matrix	1546:1568	an improved bone matrix for use in bone regeneration research	1546:1606	Collectively, this study suggests that the osteogenic protein NELL-1 showed a sustained release pattern after being encapsulated into the modified Chi/HA-TCP particles, and the NELL-1 integrated composite of Chi/HA-TCP showed a potential to function as a protein delivery carrier and as an improved bone matrix for use in bone regeneration research.
27349789	6	63	theme	NELL-1	710:715	arg1	particles					735:743	The NELL-1 loaded Chi/HA-TCP particles	706:743	The NELL-1 loaded Chi/HA-TCP particles	706:743	The NELL-1 loaded Chi/HA-TCP particles and the release kinetics were investigated in vitro.
27349789	8	64	theme	HA-TCP	1240:1245	arg1	particles					1248:1256	hydroxyapatite coated TCP (HA-TCP) particles	1213:1256	hydroxyapatite coated TCP (HA-TCP) particles	1213:1256	Furthermore, more than 78% of the loaded protein's bioactivity was preserved in Chi/HA-TCP particles over the period of the investigation, which was significantly higher than that of the protein released from hydroxyapatite coated TCP (HA-TCP) particles.
27349789	7	65	theme	NELL-1	942:947	arg1	release					931:937	the release	927:937	the release of NELL-1	927:947	It was observed that the Chi/HA-TCP particles prepared with the 0.3% (wt/wt) chitosan solution were able to successfully control the release of NELL-1 and maintain a slow, steady release for up to 28 days.
27349789	7	66	theme	%	865:865	arg1	solution					884:891	the 0.3% (wt/wt) chitosan solution	858:891	the 0.3% (wt/wt) chitosan solution	858:891	It was observed that the Chi/HA-TCP particles prepared with the 0.3% (wt/wt) chitosan solution were able to successfully control the release of NELL-1 and maintain a slow, steady release for up to 28 days.
24492609	6	0	theme	lpxL1	1201:1205	arg1	mutant					1210:1215	an lpxL1(-) mutant	1198:1215	an lpxL1(-) mutant	1198:1215	The immunomodulatory properties of PagL-deacylated LPS were compared with another pentaacyl form obtained from an lpxL1(-) mutant, which lacks the 2' secondary acyl chain.
24492609	8	1	from	present	1664:1670	arg1	/pagL					1682:1686	/pagL	1682:1686	/pagL	1682:1686	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	8	1	from	present	1664:1670	arg1	lgtB					1675:1678	lgtB	1675:1678	lgtB	1675:1678	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	8	1	from	present	1664:1670	arg1	LPS					1691:1693	LPS	1691:1693	LPS	1691:1693	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	2	2	theme	pagL	391:394	arg1	gene					396:399	the pagL gene	387:399	the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica	387:461	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	4	3	from	efficiency	773:782	arg1	hexaacyl					807:814	the hexaacyl	803:814	the hexaacyl	803:814	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	7	4	from	production	1323:1332	arg1	Mac					1404:1406	the monocytic cell line Mono Mac 6	1375:1408	the monocytic cell line Mono Mac 6	1375:1408	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	2	5	theme	Neisseria	320:328	arg1	H44/76					350:355	Neisseria meningitidis strain H44/76	320:355	Neisseria meningitidis strain H44/76	320:355	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	1	6	theme	adjuvant	299:306	arg1	properties					308:317	optimized adjuvant properties	289:317	optimized adjuvant properties	289:317	Engineering the lipopolysaccharide (LPS) biosynthetic pathway offers the potential to obtain modified derivatives with optimized adjuvant properties.
24492609	6	7	theme	acyl	1247:1250	arg1	chain					1252:1256	the 2' secondary acyl chain	1230:1256	the 2' secondary acyl chain	1230:1256	The immunomodulatory properties of PagL-deacylated LPS were compared with another pentaacyl form obtained from an lpxL1(-) mutant, which lacks the 2' secondary acyl chain.
24492609	9	8	theme	human	1869:1873	arg1	cells					1882:1886	human innate cells	1869:1886	human innate cells	1869:1886	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	5	9	theme	MS	900:901	arg1	analyses					903:910	MS analyses	900:910	MS analyses	900:910	MS analyses also provided evidence for expression of only one major oligosaccharide glycoform, which lacked the terminal galactose residue as expected from inactivation of the lgtB gene.
24492609	8	10	theme	LPS	1786:1788	arg1	activity					1769:1776	the activity	1765:1776	the activity of this LPS	1765:1788	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	7	11	theme	protein	1545:1551	arg1	induction					1411:1419	induction	1411:1419	induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1411:1554	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	8	12	from	lgtB	1675:1678	arg1	present					1664:1670	present	1664:1670	present	1664:1670	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	5	13	theme	major	962:966	arg1	glycoform					984:992	only one major oligosaccharide glycoform	953:992	only one major oligosaccharide glycoform	953:992	MS analyses also provided evidence for expression of only one major oligosaccharide glycoform, which lacked the terminal galactose residue as expected from inactivation of the lgtB gene.
24492609	7	14	theme	chemokine	1514:1522	arg1	protein					1545:1551	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	9	15	theme	detailed	1953:1960	arg1	analysis					1981:1988	detailed structure-function analysis	1953:1988	detailed structure-function analysis when working with modified lipid A structures	1953:2034	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	4	16	theme	modified	715:722	arg1	LPS					724:726	the modified LPS	711:726	the modified LPS	711:726	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	3	17	theme	compositional	633:645	arg1	analysis					647:654	detailed compositional analysis	624:654	detailed compositional analysis of all present molecular species	624:687	Mass spectrometry analysis of purified mutant LPS was used for detailed compositional analysis of all present molecular species.
24492609	7	18	theme	adaptor-inducing	1474:1489	arg1	protein					1545:1551	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	9	19	theme	analysis	1981:1988	arg1	importance					1939:1948	the importance	1935:1948	the importance of detailed structure-function analysis when working with modified lipid A structures	1935:2034	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	0	20	theme	modified	141:148	arg1	properties					158:167	their modified agonist properties	135:167	their modified agonist properties	135:167	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	3	21	theme	species	681:687	arg1	analysis					647:654	detailed compositional analysis	624:654	detailed compositional analysis of all present molecular species	624:687	Mass spectrometry analysis of purified mutant LPS was used for detailed compositional analysis of all present molecular species.
24492609	5	22	gly	glycoform	984:992	arg1	oligosaccharide					968:982	only one major oligosaccharide glycoform	953:992	only one major oligosaccharide glycoform	953:992	MS analyses also provided evidence for expression of only one major oligosaccharide glycoform, which lacked the terminal galactose residue as expected from inactivation of the lgtB gene.
24492609	7	23	theme	receptor	1447:1454	arg1	protein					1545:1551	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	0	24	theme	properties	158:167	arg1	comparison					121:130	comparison	121:130	comparison of their modified agonist properties	121:167	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	0	24	theme	properties	158:167	arg1	analysis					69:76	structural analysis	58:76	structural analysis of different pentaacyl lipid A mutants	58:115	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	3	25	theme	present	663:669	arg1	species					681:687	all present molecular species	659:687	all present molecular species	659:687	Mass spectrometry analysis of purified mutant LPS was used for detailed compositional analysis of all present molecular species.
24492609	8	26	attach	present	1664:1670	arg2	species					1644:1650	remaining hexaacyl species	1625:1650	remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS	1625:1693	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	8	26	attach	present	1664:1670	arg1	/pagL					1682:1686	/pagL	1682:1686	/pagL	1682:1686	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	8	26	attach	present	1664:1670	arg1	lgtB					1675:1678	lgtB	1675:1678	lgtB	1675:1678	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	8	26	attach	present	1664:1670	arg1	LPS					1691:1693	LPS	1691:1693	LPS	1691:1693	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	9	27	theme	modified	2008:2015	arg1	structures					2025:2034	modified lipid A structures	2008:2034	modified lipid A structures	2008:2034	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	8	28	from	LPS	1691:1693	arg1	present					1664:1670	present	1664:1670	present	1664:1670	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	5	29	theme	galactose	1021:1029	arg1	residue					1031:1037	the terminal galactose residue	1008:1037	the terminal galactose residue	1008:1037	MS analyses also provided evidence for expression of only one major oligosaccharide glycoform, which lacked the terminal galactose residue as expected from inactivation of the lgtB gene.
24492609	6	30	theme	PagL-deacylated	1122:1136	arg1	LPS					1138:1140	PagL-deacylated LPS	1122:1140	PagL-deacylated LPS	1122:1140	The immunomodulatory properties of PagL-deacylated LPS were compared with another pentaacyl form obtained from an lpxL1(-) mutant, which lacks the 2' secondary acyl chain.
24492609	9	31	theme	A	2023:2023	arg1	structures					2025:2034	modified lipid A structures	2008:2034	modified lipid A structures	2008:2034	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	9	32	theme	cells	1882:1886	arg1	response					1857:1864	a qualitatively different response	1831:1864	a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS	1831:1924	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	5	33	theme	glycoform	984:992	arg1	expression					939:948	expression	939:948	expression of only one major oligosaccharide glycoform, which lacked the terminal galactose residue as expected from inactivation of the lgtB gene	939:1084	MS analyses also provided evidence for expression of only one major oligosaccharide glycoform, which lacked the terminal galactose residue as expected from inactivation of the lgtB gene.
24492609	4	34	theme	conversion	787:796	arg1	efficiency					773:782	high efficiency	768:782	high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression	768:897	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	10	35	theme	significant	2057:2067	arg1	potential					2069:2077	significant potential	2057:2077	significant potential	2057:2077	The pagL(+) LPS has significant potential as immune modulator in humans.
24492609	0	36	theme	pentaacyl	91:99	arg1	mutants					109:115	different pentaacyl lipid A mutants	81:115	different pentaacyl lipid A mutants	81:115	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	4	37	theme	lipid	859:863	arg1	PagL					882:885	PagL	882:885	PagL	882:885	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	4	37	theme	lipid	859:863	arg1	3-O-deacylase					867:879	heterologous lipid A 3-O-deacylase	846:879	heterologous lipid A 3-O-deacylase (PagL) expression	846:897	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	0	38	theme	A	107:107	arg1	mutants					109:115	different pentaacyl lipid A mutants	81:115	different pentaacyl lipid A mutants	81:115	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	9	39	theme	different	1847:1855	arg1	response					1857:1864	a qualitatively different response	1831:1864	a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS	1831:1924	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	4	40	theme	3-O-deacylase	867:879	arg1	expression					888:897	heterologous lipid A 3-O-deacylase (PagL) expression	846:897	heterologous lipid A 3-O-deacylase (PagL) expression	846:897	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	5	41	theme	lgtB	1076:1079	arg1	gene					1081:1084	the lgtB gene	1072:1084	the lgtB gene	1072:1084	MS analyses also provided evidence for expression of only one major oligosaccharide glycoform, which lacked the terminal galactose residue as expected from inactivation of the lgtB gene.
24492609	2	42	theme	gene	495:498	arg1	inactivation					470:481	inactivation	470:481	inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase	470:558	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	8	43	theme	remaining	1625:1633	arg1	species					1644:1650	remaining hexaacyl species	1625:1650	remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS	1625:1693	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	4	44	theme	3-O-deacylated	823:836	arg1	form					838:841	the 3-O-deacylated form	819:841	the 3-O-deacylated form	819:841	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	0	45	theme	Lipopolysaccharide	0:17	arg1	engineering					19:29	Lipopolysaccharide engineering	0:29	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.	0:168	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	6	46	theme	pentaacyl	1169:1177	arg1	form					1179:1182	another pentaacyl form	1161:1182	another pentaacyl form obtained from an lpxL1(-) mutant, which lacks the 2' secondary acyl chain	1161:1256	The immunomodulatory properties of PagL-deacylated LPS were compared with another pentaacyl form obtained from an lpxL1(-) mutant, which lacks the 2' secondary acyl chain.
24492609	9	47	theme	innate	1875:1880	arg1	cells					1882:1886	human innate cells	1869:1886	human innate cells	1869:1886	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	8	48	theme	species	1644:1650	arg1	Removal					1614:1620	Removal	1614:1620	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS	1614:1693	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	7	49	theme	line	1394:1397	arg1	Mac					1404:1406	the monocytic cell line Mono Mac 6	1375:1408	the monocytic cell line Mono Mac 6	1375:1408	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	2	50	theme	oligosaccharide	522:536	arg1	galactosyltransferase					538:558	the terminal oligosaccharide galactosyltransferase	509:558	the terminal oligosaccharide galactosyltransferase	509:558	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	8	51	theme	present	1664:1670	arg1	species					1644:1650	remaining hexaacyl species	1625:1650	remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS	1625:1693	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	9	52	theme	pentaacyl	1891:1899	arg1	lpxL1					1901:1905	pentaacyl lpxL1	1891:1905	pentaacyl lpxL1	1891:1905	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	7	53	theme	monocytic	1379:1387	arg1	Mac					1404:1406	the monocytic cell line Mono Mac 6	1375:1408	the monocytic cell line Mono Mac 6	1375:1408	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	9	54	theme	lpxL1	1901:1905	arg1	LPS					1922:1924	pentaacyl lpxL1(-) and pagL(+) LPS	1891:1924	pentaacyl lpxL1(-) and pagL(+) LPS	1891:1924	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	3	55	theme	Mass	561:564	arg1	analysis					579:586	Mass spectrometry analysis	561:586	Mass spectrometry analysis of purified mutant LPS	561:609	Mass spectrometry analysis of purified mutant LPS was used for detailed compositional analysis of all present molecular species.
24492609	0	56	theme	structural	58:67	arg1	analysis					69:76	structural analysis	58:76	structural analysis of different pentaacyl lipid A mutants	58:115	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	1	57	with	derivatives	272:282	arg1	properties					308:317	optimized adjuvant properties	289:317	optimized adjuvant properties	289:317	Engineering the lipopolysaccharide (LPS) biosynthetic pathway offers the potential to obtain modified derivatives with optimized adjuvant properties.
24492609	4	58	from	hexaacyl	807:814	arg1	conversion					787:796	conversion	787:796	conversion from the hexaacyl to the 3-O-deacylated form	787:841	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	4	58	from	hexaacyl	807:814	arg1	efficiency					773:782	high efficiency	768:782	high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression	768:897	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	7	59	theme	cytokine	1358:1365	arg1	IL-6					1367:1370	the pro-inflammatory cytokine IL-6	1337:1370	the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6	1337:1408	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	3	60	theme	purified	591:598	arg1	LPS					607:609	purified mutant LPS	591:609	purified mutant LPS	591:609	Mass spectrometry analysis of purified mutant LPS was used for detailed compositional analysis of all present molecular species.
24492609	7	61	theme	/pagL	1597:1601	arg1	mutant					1606:1611	the lgtB(-)/pagL(+) mutant	1586:1611	the lgtB(-)/pagL(+) mutant	1586:1611	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	2	62	theme	gene	396:399	arg1	expression					373:382	expression	373:382	expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica	373:461	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	2	63	theme	meningitidis	330:341	arg1	H44/76					350:355	Neisseria meningitidis strain H44/76	320:355	Neisseria meningitidis strain H44/76	320:355	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	1	64	theme	optimized	289:297	arg1	properties					308:317	optimized adjuvant properties	289:317	optimized adjuvant properties	289:317	Engineering the lipopolysaccharide (LPS) biosynthetic pathway offers the potential to obtain modified derivatives with optimized adjuvant properties.
24492609	7	65	theme	lgtB	1590:1593	arg1	mutant					1606:1611	the lgtB(-)/pagL(+) mutant	1586:1611	the lgtB(-)/pagL(+) mutant	1586:1611	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	3	66	theme	LPS	607:609	arg1	analysis					579:586	Mass spectrometry analysis	561:586	Mass spectrometry analysis of purified mutant LPS	561:609	Mass spectrometry analysis of purified mutant LPS was used for detailed compositional analysis of all present molecular species.
24492609	1	67	theme	lipopolysaccharide	186:203	arg1	pathway					224:230	Engineering the lipopolysaccharide (LPS) biosynthetic pathway	170:230	Engineering the lipopolysaccharide (LPS) biosynthetic pathway	170:230	Engineering the lipopolysaccharide (LPS) biosynthetic pathway offers the potential to obtain modified derivatives with optimized adjuvant properties.
24492609	6	68	theme	secondary	1237:1245	arg1	chain					1252:1256	the 2' secondary acyl chain	1230:1256	the 2' secondary acyl chain	1230:1256	The immunomodulatory properties of PagL-deacylated LPS were compared with another pentaacyl form obtained from an lpxL1(-) mutant, which lacks the 2' secondary acyl chain.
24492609	7	69	theme	impaired	1295:1302	arg1	capacity					1304:1311	impaired capacity	1295:1311	impaired capacity	1295:1311	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	7	70	from	IL-6	1367:1370	arg1	Mac					1404:1406	the monocytic cell line Mono Mac 6	1375:1408	the monocytic cell line Mono Mac 6	1375:1408	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	3	71	used	used	615:618	arg2	analysis					579:586	Mass spectrometry analysis	561:586	Mass spectrometry analysis of purified mutant LPS	561:609	Mass spectrometry analysis of purified mutant LPS was used for detailed compositional analysis of all present molecular species.
24492609	3	72	theme	detailed	624:631	arg1	analysis					647:654	detailed compositional analysis	624:654	detailed compositional analysis of all present molecular species	624:687	Mass spectrometry analysis of purified mutant LPS was used for detailed compositional analysis of all present molecular species.
24492609	1	73	theme	biosynthetic	211:222	arg1	pathway					224:230	Engineering the lipopolysaccharide (LPS) biosynthetic pathway	170:230	Engineering the lipopolysaccharide (LPS) biosynthetic pathway	170:230	Engineering the lipopolysaccharide (LPS) biosynthetic pathway offers the potential to obtain modified derivatives with optimized adjuvant properties.
24492609	7	74	theme	interferon-γ-induced	1524:1543	arg1	protein					1545:1551	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	9	75	theme	structure-function	1962:1979	arg1	analysis					1981:1988	detailed structure-function analysis	1953:1988	detailed structure-function analysis when working with modified lipid A structures	1953:2034	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	0	76	theme	agonist	150:156	arg1	properties					158:167	their modified agonist properties	135:167	their modified agonist properties	135:167	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	3	77	theme	molecular	671:679	arg1	species					681:687	all present molecular species	659:687	all present molecular species	659:687	Mass spectrometry analysis of purified mutant LPS was used for detailed compositional analysis of all present molecular species.
24492609	7	78	theme	interferon-β-dependent	1491:1512	arg1	protein					1545:1551	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	7	79	theme	LPS	1273:1275	arg1	mutants					1277:1283	both LPS mutants	1268:1283	both LPS mutants	1268:1283	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	7	80	theme	domain-containing	1456:1472	arg1	protein					1545:1551	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	5	81	theme	terminal	1012:1019	arg1	residue					1031:1037	the terminal galactose residue	1008:1037	the terminal galactose residue	1008:1037	MS analyses also provided evidence for expression of only one major oligosaccharide glycoform, which lacked the terminal galactose residue as expected from inactivation of the lgtB gene.
24492609	6	82	theme	LPS	1138:1140	arg1	properties					1108:1117	The immunomodulatory properties	1087:1117	The immunomodulatory properties of PagL-deacylated LPS	1087:1140	The immunomodulatory properties of PagL-deacylated LPS were compared with another pentaacyl form obtained from an lpxL1(-) mutant, which lacks the 2' secondary acyl chain.
24492609	7	83	theme	Toll-interleukin-1	1428:1445	arg1	protein					1545:1551	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10	1424:1554	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	9	84	theme	lipid	2017:2021	arg1	structures					2025:2034	modified lipid A structures	2008:2034	modified lipid A structures	2008:2034	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	0	85	theme	different	81:89	arg1	mutants					109:115	different pentaacyl lipid A mutants	81:115	different pentaacyl lipid A mutants	81:115	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	5	86	theme	oligosaccharide	968:982	arg1	glycoform					984:992	only one major oligosaccharide glycoform	953:992	only one major oligosaccharide glycoform	953:992	MS analyses also provided evidence for expression of only one major oligosaccharide glycoform, which lacked the terminal galactose residue as expected from inactivation of the lgtB gene.
24492609	8	87	theme	minor	1719:1723	arg1	species					1725:1731	these minor species	1713:1731	these minor species	1713:1731	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	4	88	theme	high	768:771	arg1	efficiency					773:782	high efficiency	768:782	high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression	768:897	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	0	89	theme	lipid	101:105	arg1	mutants					109:115	different pentaacyl lipid A mutants	81:115	different pentaacyl lipid A mutants	81:115	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	1	90	theme	Engineering	170:180	arg1	pathway					224:230	Engineering the lipopolysaccharide (LPS) biosynthetic pathway	170:230	Engineering the lipopolysaccharide (LPS) biosynthetic pathway	170:230	Engineering the lipopolysaccharide (LPS) biosynthetic pathway offers the potential to obtain modified derivatives with optimized adjuvant properties.
24492609	6	91	theme	immunomodulatory	1091:1106	arg1	properties					1108:1117	The immunomodulatory properties	1087:1117	The immunomodulatory properties of PagL-deacylated LPS	1087:1140	The immunomodulatory properties of PagL-deacylated LPS were compared with another pentaacyl form obtained from an lpxL1(-) mutant, which lacks the 2' secondary acyl chain.
24492609	0	92	from	engineering	19:29	arg1	meningitidis					44:55	Neisseria meningitidis	34:55	Neisseria meningitidis	34:55	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	0	93	theme	mutants	109:115	arg1	comparison					121:130	comparison	121:130	comparison of their modified agonist properties	121:167	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	0	93	theme	mutants	109:115	arg1	analysis					69:76	structural analysis	58:76	structural analysis of different pentaacyl lipid A mutants	58:115	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	2	94	theme	strain	343:348	arg1	H44/76					350:355	Neisseria meningitidis strain H44/76	320:355	Neisseria meningitidis strain H44/76	320:355	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	4	95	theme	heterologous	846:857	arg1	PagL					882:885	PagL	882:885	PagL	882:885	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	4	95	theme	heterologous	846:857	arg1	3-O-deacylase					867:879	heterologous lipid A 3-O-deacylase	846:879	heterologous lipid A 3-O-deacylase (PagL) expression	846:897	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	8	96	theme	hexaacyl	1635:1642	arg1	species					1644:1650	remaining hexaacyl species	1625:1650	remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS	1625:1693	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	4	97	theme	A	865:865	arg1	PagL					882:885	PagL	882:885	PagL	882:885	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	4	97	theme	A	865:865	arg1	3-O-deacylase					867:879	heterologous lipid A 3-O-deacylase	846:879	heterologous lipid A 3-O-deacylase (PagL) expression	846:897	This determined that the modified LPS was mainly pentaacylated, demonstrating high efficiency of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (PagL) expression.
24492609	7	98	theme	Mono	1399:1402	arg1	Mac					1404:1406	the monocytic cell line Mono Mac 6	1375:1408	the monocytic cell line Mono Mac 6	1375:1408	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	2	99	theme	lgtB	490:493	arg1	gene					495:498	the lgtB gene	486:498	the lgtB gene encoding the terminal oligosaccharide galactosyltransferase	486:558	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	10	100	contain	has	2053:2055	arg2	potential					2069:2077	significant potential	2057:2077	significant potential	2057:2077	The pagL(+) LPS has significant potential as immune modulator in humans.
24492609	10	100	contain	has	2053:2055	arg1	pagL					2041:2044	The pagL	2037:2044	The pagL	2037:2044	The pagL(+) LPS has significant potential as immune modulator in humans.
24492609	10	100	contain	has	2053:2055	arg1	LPS					2049:2051	LPS	2049:2051	LPS	2049:2051	The pagL(+) LPS has significant potential as immune modulator in humans.
24492609	2	101	theme	A	416:416	arg1	3-O-deacylase					418:430	lipid A 3-O-deacylase	410:430	lipid A 3-O-deacylase from Bordetella bronchiseptica	410:461	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	0	102	theme	Neisseria	34:42	arg1	meningitidis					44:55	Neisseria meningitidis	34:55	Neisseria meningitidis	34:55	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	2	103	mod	modified	361:368	arg1	H44/76					350:355	Neisseria meningitidis strain H44/76	320:355	Neisseria meningitidis strain H44/76	320:355	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	2	103	mod	modified	361:368	arg3	expression					373:382	expression	373:382	expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica	373:461	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	7	104	theme	cell	1389:1392	arg1	Mac					1404:1406	the monocytic cell line Mono Mac 6	1375:1408	the monocytic cell line Mono Mac 6	1375:1408	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	7	105	from	Mac	1404:1406	arg1	production					1323:1332	production	1323:1332	production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6	1323:1408	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	10	106	from	modulator	2089:2097	arg1	humans					2102:2107	humans	2102:2107	humans	2102:2107	The pagL(+) LPS has significant potential as immune modulator in humans.
24492609	2	107	theme	lipid	410:414	arg1	3-O-deacylase					418:430	lipid A 3-O-deacylase	410:430	lipid A 3-O-deacylase from Bordetella bronchiseptica	410:461	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	2	108	theme	terminal	513:520	arg1	galactosyltransferase					538:558	the terminal oligosaccharide galactosyltransferase	509:558	the terminal oligosaccharide galactosyltransferase	509:558	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	7	109	theme	IL-6	1367:1370	arg1	production					1323:1332	production	1323:1332	production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6	1323:1408	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	8	110	from	/pagL	1682:1686	arg1	present					1664:1670	present	1664:1670	present	1664:1670	Removal of remaining hexaacyl species exclusively present in lgtB(-)/pagL(+) LPS demonstrated that these minor species potentiate but do not determine the activity of this LPS.
24492609	3	111	theme	spectrometry	566:577	arg1	analysis					579:586	Mass spectrometry analysis	561:586	Mass spectrometry analysis of purified mutant LPS	561:609	Mass spectrometry analysis of purified mutant LPS was used for detailed compositional analysis of all present molecular species.
24492609	5	112	theme	gene	1081:1084	arg1	inactivation					1056:1067	inactivation	1056:1067	inactivation of the lgtB gene	1056:1084	MS analyses also provided evidence for expression of only one major oligosaccharide glycoform, which lacked the terminal galactose residue as expected from inactivation of the lgtB gene.
24492609	7	113	theme	pro-inflammatory	1341:1356	arg1	IL-6					1367:1370	the pro-inflammatory cytokine IL-6	1337:1370	the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6	1337:1408	Although both LPS mutants displayed impaired capacity to induce production of the pro-inflammatory cytokine IL-6 in the monocytic cell line Mono Mac 6, induction of the Toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-β-dependent chemokine interferon-γ-induced protein 10 was largely retained only for the lgtB(-)/pagL(+) mutant.
24492609	10	114	theme	immune	2082:2087	arg1	modulator					2089:2097	immune modulator	2082:2097	immune modulator in humans	2082:2107	The pagL(+) LPS has significant potential as immune modulator in humans.
24492609	0	115	dep	engineering	19:29	arg1	analysis					69:76	structural analysis	58:76	structural analysis of different pentaacyl lipid A mutants	58:115	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	0	115	dep	engineering	19:29	arg1	comparison					121:130	comparison	121:130	comparison of their modified agonist properties	121:167	Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
24492609	2	116	from	bronchiseptica	448:461	arg1	3-O-deacylase					418:430	lipid A 3-O-deacylase	410:430	lipid A 3-O-deacylase from Bordetella bronchiseptica	410:461	Neisseria meningitidis strain H44/76 was modified by expression of the pagL gene encoding lipid A 3-O-deacylase from Bordetella bronchiseptica and by inactivation of the lgtB gene encoding the terminal oligosaccharide galactosyltransferase.
24492609	9	117	theme	pagL	1914:1917	arg1	LPS					1922:1924	pentaacyl lpxL1(-) and pagL(+) LPS	1891:1924	pentaacyl lpxL1(-) and pagL(+) LPS	1891:1924	These results are the first to indicate a qualitatively different response of human innate cells to pentaacyl lpxL1(-) and pagL(+) LPS and show the importance of detailed structure-function analysis when working with modified lipid A structures.
24492609	3	118	theme	mutant	600:605	arg1	LPS					607:609	purified mutant LPS	591:609	purified mutant LPS	591:609	Mass spectrometry analysis of purified mutant LPS was used for detailed compositional analysis of all present molecular species.
24492609	1	119	theme	modified	263:270	arg1	derivatives					272:282	modified derivatives	263:282	modified derivatives with optimized adjuvant properties	263:317	Engineering the lipopolysaccharide (LPS) biosynthetic pathway offers the potential to obtain modified derivatives with optimized adjuvant properties.